                         SEQUENCE LISTING

<110>  CureVac AG
 
<120>  Nucleic acids encoding CRISPR-associated proteins and uses 
       thereof

<130>  CU01P245WO1

<160>  14546 

<170>  PatentIn version 3.5

<210>  1
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_HSD17B4

<400>  1
gtcccgcagt cggcgtccag cggctctgct tgttcgtgtg tgtgtcgttg caggccttat       60

tc                                                                      62


<210>  2
<211>  62
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_HSD17B4

<400>  2
gucccgcagu cggcguccag cggcucugcu uguucgugug ugugucguug caggccuuau       60

uc                                                                      62


<210>  3
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_ASAH1

<400>  3
gcctctgctg gagtccgggg agtggcgttg gctgctagag cg                          42


<210>  4
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_ASAH1

<400>  4
gccucugcug gaguccgggg aguggcguug gcugcuagag cg                          42


<210>  5
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_ATP5A1

<400>  5
gcggctcggc cattttgtcc cagtcagtcc ggaggctgcg gctgcagaag taccgcctgc       60

ggagtaactg caaag                                                        75


<210>  6
<211>  75
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_ATP5A1

<400>  6
gcggcucggc cauuuugucc cagucagucc ggaggcugcg gcugcagaag uaccgccugc       60

ggaguaacug caaag                                                        75


<210>  7
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Mp68

<400>  7
ctttcccatt ctgtagcaga atttggtgtt gcctgtggtc ttggtcccgc ggag             54


<210>  8
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Mp68

<400>  8
cuuucccauu cuguagcaga auuugguguu gccugugguc uuggucccgc ggag             54


<210>  9
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Ndufa4

<400>  9
gtccgctcag ccaggttgca gaagcggctt agcgtgtgtc ctaatcttct ctctgcgtgt       60

aggtaggcct gtgccgcaaa c                                                 81


<210>  10
<211>  81
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Ndufa4

<400>  10
guccgcucag ccagguugca gaagcggcuu agcguguguc cuaaucuucu cucugcgugu       60

agguaggccu gugccgcaaa c                                                 81


<210>  11
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Nosip1

<400>  11
ctcctgtcgg gcggaagtag gaggagtaga gtttaaaaac agtactcttt ttccggttcg       60

ggacgtagtt gaagcaacga caagccggat aaccgctctt gagacagg                   108


<210>  12
<211>  108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Nosip1

<400>  12
cuccugucgg gcggaaguag gaggaguaga guuuaaaaac aguacucuuu uuccgguucg       60

ggacguaguu gaagcaacga caagccggau aaccgcucuu gagacagg                   108


<210>  13
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_RPL31.1

<400>  13
cccgtgaccc ggaagttgta cggctacgcg actttccctc ccacaaaccc tcgcgccctt       60

cctttcctac ttgggcccgg caga                                              84


<210>  14
<211>  84
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_RPL31.1

<400>  14
cccgugaccc ggaaguugua cggcuacgcg acuuucccuc ccacaaaccc ucgcgcccuu       60

ccuuuccuac uugggcccgg caga                                              84


<210>  15
<211>  159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Slc7a3.1

<400>  15
gggcgcttgg cttgcaagga ccctgagctg cggcattgaa gcacacccaa cccaactcga       60

ctgaagtcag cctcactgaa ccggatctga gaatcttctc tctctgggct tgccagggct      120

ctccgaacct agctagcatc ctcttcaatt ccaactaga                             159


<210>  16
<211>  159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Slc7a3.1

<400>  16
gggcgcuugg cuugcaagga cccugagcug cggcauugaa gcacacccaa cccaacucga       60

cugaagucag ccucacugaa ccggaucuga gaaucuucuc ucucugggcu ugccagggcu      120

cuccgaaccu agcuagcauc cucuucaauu ccaacuaga                             159


<210>  17
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_TUBB4B

<400>  17
atataagcgt tggcggagcg tcggttgtag cactctgcgc gcccgctctt ctgctgctgt       60

ttgtctactt cctcctgctt ccccgccgcc gccgccgcca tc                         102


<210>  18
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_TUBB4B

<400>  18
auauaagcgu uggcggagcg ucgguuguag cacucugcgc gcccgcucuu cugcugcugu       60

uugucuacuu ccuccugcuu ccccgccgcc gccgccgcca uc                         102


<210>  19
<211>  222
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Ubqln2

<400>  19
cggagacggc ctgcaggacc tgctctctca gccctcagcc gaggcctacg ccgagccgag       60

tgcgcagccg acgaccggga ggagccgcag ccttcaactc tgaggtactg tgatccgcgc      120

tgcccgccgg gccgccccag tccgctgctg cggcacctcc ttccctcgcg ccctcttcgc      180

tcgccagcgc cttccctgtg agcctgcgtc accgcggccg cc                         222


<210>  20
<211>  222
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Ubqln2

<400>  20
cggagacggc cugcaggacc ugcucucuca gcccucagcc gaggccuacg ccgagccgag       60

ugcgcagccg acgaccggga ggagccgcag ccuucaacuc ugagguacug ugauccgcgc      120

ugcccgccgg gccgccccag uccgcugcug cggcaccucc uucccucgcg cccucuucgc      180

ucgccagcgc cuucccugug agccugcguc accgcggccg cc                         222


<210>  21
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_RPL32

<400>  21
ggcgctgcct acggaggtgg cagccatctc cttctcggca tc                          42


<210>  22
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_RPL32

<400>  22
ggcgcugccu acggaggugg cagccaucuc cuucucggca uc                          42


<210>  23
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_PSMB3.1

<400>  23
ccctgttccc agagcccact tttttttctt tttttgaaat aaaatagcct gtctttc          57


<210>  24
<211>  57
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_PSMB3.1

<400>  24
cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuuc          57


<210>  25
<211>  121
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_CASP1

<400>  25
aataaggaaa ctgtatgaat gtctgtgggc aggaagtgaa gagatccttc tgtaaaggtt       60

tttggaatta tgtctgctga ataataaact tttttgaaat aataaatctg gtagaaaaat      120

g                                                                      121


<210>  26
<211>  121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_CASP1

<400>  26
aauaaggaaa cuguaugaau gucugugggc aggaagugaa gagauccuuc uguaaagguu       60

uuuggaauua ugucugcuga auaauaaacu uuuuugaaau aauaaaucug guagaaaaau      120

g                                                                      121


<210>  27
<211>  137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_COXB1

<400>  27
actggctgca tctccctttc ctctgtcctc catccttctc ccaggatggt gaagggggac       60

ctggtaccca gtgatcccca ccccaggatc ctaaatcatg acttacctgc taataaaaac      120

tcattggaaa agtgaga                                                     137


<210>  28
<211>  137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_COXB1

<400>  28
acuggcugca ucucccuuuc cucuguccuc cauccuucuc ccaggauggu gaagggggac       60

cugguaccca gugaucccca ccccaggauc cuaaaucaug acuuaccugc uaauaaaaac      120

ucauuggaaa agugaga                                                     137


<210>  29
<211>  353
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_Gnas

<400>  29
gaagggaaca cccaaattta attcagcctt aagcacaatt aattaagagt gaaacgtaat       60

tgtacaagca gttggtcacc caccataggg catgatcaac accgcaacct ttcctttttc      120

ccccagtgat tctgaaaaac ccctcttccc ttcagcttgc ttagatgttc caaatttagt      180

aagcttaagg cggcctacag aagaaaaaga aaaaaaaggc cacaaaagtt ccctctcact      240

ttcagtaaat aaaataaaag cagcaacaga aataaagaaa taaatgaaat tcaaaatgaa      300

ataaatattg tgttgtgcag cattaaaaaa tcaataaaaa ttaaaaatga gca             353


<210>  30
<211>  353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_Gnas

<400>  30
gaagggaaca cccaaauuua auucagccuu aagcacaauu aauuaagagu gaaacguaau       60

uguacaagca guuggucacc caccauaggg caugaucaac accgcaaccu uuccuuuuuc      120

ccccagugau ucugaaaaac cccucuuccc uucagcuugc uuagauguuc caaauuuagu      180

aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu cccucucacu      240

uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau ucaaaaugaa      300

auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga gca             353


<210>  31
<211>  133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_Ndufa1

<400>  31
ggaagcattt tcctggctga ttaaaagaaa ttactcagct atggtcatct gttcctgtta       60

gaaggctatg cagcatatta tatactatgc gcatgttatg aaatgcataa taaaaaattt      120

taaaaaatct aaa                                                         133


<210>  32
<211>  133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_Ndufa1

<400>  32
ggaagcauuu uccuggcuga uuaaaagaaa uuacucagcu auggucaucu guuccuguua       60

gaaggcuaug cagcauauua uauacuaugc gcauguuaug aaaugcauaa uaaaaaauuu      120

uaaaaaaucu aaa                                                         133


<210>  33
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_RPS9.1

<400>  33
gtccacctgt ccctcctggg ctgctggatt gtctcgtttt cctgccaaat aaacaggatc       60

agcgctttac                                                              70


<210>  34
<211>  70
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_RPS9.1

<400>  34
guccaccugu cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc       60

agcgcuuuac                                                              70


<210>  35
<211>  187
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_ALB7.1

<400>  35
gcatcacatt taaaagcatc tcagcctacc atgagaataa gagaaagaaa atgaagatca       60

atagcttatt catctctttt tctttttcgt tggtgtaaag ccaacaccct gtctaaaaaa      120

cataaatttc tttaatcatt ttgcctcttt tctctgtgct tcaattaata aaaaatggaa      180

agaacct                                                                187


<210>  36
<211>  187
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_ALB7.1

<400>  36
gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa augaagauca       60

auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa      120

cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa      180

agaaccu                                                                187


<210>  37
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  histone stem-loop

<400>  37
caaaggctct tttcagagcc acca                                              24


<210>  38
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  histone stem-loop

<400>  38
caaaggcucu uuucagagcc acca                                              24


<210>  39
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kozak

<400>  39
gccgccacca tgg                                                          13


<210>  40
<211>  13
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Kozak

<400>  40
gccgccacca ugg                                                          13


<210>  41
<211>  6
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-End

<400>  41
gggaga                                                                   6


<210>  42
<211>  524
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RAV-G

<400>  42

Met Val Pro Gln Ala Leu Leu Phe Val Pro Leu Leu Val Phe Pro Leu 
1               5                   10                  15      


Cys Phe Gly Lys Phe Pro Ile Tyr Thr Ile Pro Asp Lys Leu Gly Pro 
            20                  25                  30          


Trp Ser Pro Ile Asp Ile His His Leu Ser Cys Pro Asn Asn Leu Val 
        35                  40                  45              


Val Glu Asp Glu Gly Cys Thr Asn Leu Ser Gly Phe Ser Tyr Met Glu 
    50                  55                  60                  


Leu Lys Val Gly Tyr Ile Ser Ala Ile Lys Met Asn Gly Phe Thr Cys 
65                  70                  75                  80  


Thr Gly Val Val Thr Glu Ala Glu Thr Tyr Thr Asn Phe Val Gly Tyr 
                85                  90                  95      


Val Thr Thr Thr Phe Lys Arg Lys His Phe Arg Pro Thr Pro Asp Ala 
            100                 105                 110         


Cys Arg Ala Ala Tyr Asn Trp Lys Met Ala Gly Asp Pro Arg Tyr Glu 
        115                 120                 125             


Glu Ser Leu His Asn Pro Tyr Pro Asp Tyr His Trp Leu Arg Thr Val 
    130                 135                 140                 


Lys Thr Thr Lys Glu Ser Leu Val Ile Ile Ser Pro Ser Val Ala Asp 
145                 150                 155                 160 


Leu Asp Pro Tyr Asp Arg Ser Leu His Ser Arg Val Phe Pro Gly Gly 
                165                 170                 175     


Asn Cys Ser Gly Val Ala Val Ser Ser Thr Tyr Cys Ser Thr Asn His 
            180                 185                 190         


Asp Tyr Thr Ile Trp Met Pro Glu Asn Pro Arg Leu Gly Met Ser Cys 
        195                 200                 205             


Asp Ile Phe Thr Asn Ser Arg Gly Lys Arg Ala Ser Lys Gly Ser Glu 
    210                 215                 220                 


Thr Cys Gly Phe Val Asp Glu Arg Gly Leu Tyr Lys Ser Leu Lys Gly 
225                 230                 235                 240 


Ala Cys Lys Leu Lys Leu Cys Gly Val Leu Gly Leu Arg Leu Met Asp 
                245                 250                 255     


Gly Thr Trp Val Ala Met Gln Thr Ser Asn Glu Thr Lys Trp Cys Pro 
            260                 265                 270         


Pro Gly Gln Leu Val Asn Leu His Asp Phe Arg Ser Asp Glu Ile Glu 
        275                 280                 285             


His Leu Val Val Glu Glu Leu Val Lys Lys Arg Glu Glu Cys Leu Asp 
    290                 295                 300                 


Ala Leu Glu Ser Ile Met Thr Thr Lys Ser Val Ser Phe Arg Arg Leu 
305                 310                 315                 320 


Ser His Leu Arg Lys Leu Val Pro Gly Phe Gly Lys Ala Tyr Thr Ile 
                325                 330                 335     


Phe Asn Lys Thr Leu Met Glu Ala Asp Ala His Tyr Lys Ser Val Arg 
            340                 345                 350         


Thr Trp Asn Glu Ile Ile Pro Ser Lys Gly Cys Leu Arg Val Gly Gly 
        355                 360                 365             


Arg Cys His Pro His Val Asn Gly Val Phe Phe Asn Gly Ile Ile Leu 
    370                 375                 380                 


Gly Pro Asp Gly Asn Val Leu Ile Pro Glu Met Gln Ser Ser Leu Leu 
385                 390                 395                 400 


Gln Gln His Met Glu Leu Leu Val Ser Ser Val Ile Pro Leu Met His 
                405                 410                 415     


Pro Leu Ala Asp Pro Ser Thr Val Phe Lys Asn Gly Asp Glu Ala Glu 
            420                 425                 430         


Asp Phe Val Glu Val His Leu Pro Asp Val His Glu Arg Ile Ser Gly 
        435                 440                 445             


Val Asp Leu Gly Leu Pro Asn Trp Gly Lys Tyr Val Leu Leu Ser Ala 
    450                 455                 460                 


Gly Ala Leu Thr Ala Leu Met Leu Ile Ile Phe Leu Met Thr Cys Trp 
465                 470                 475                 480 


Arg Arg Val Asn Arg Ser Glu Pro Thr Gln His Asn Leu Arg Gly Thr 
                485                 490                 495     


Gly Arg Glu Val Ser Val Thr Pro Gln Ser Gly Lys Ile Ile Ser Ser 
            500                 505                 510         


Trp Glu Ser Tyr Lys Ser Gly Gly Glu Thr Gly Leu 
        515                 520                 


<210>  43
<211>  193
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HsEPO

<400>  43

Met Gly Val His Glu Cys Pro Ala Trp Leu Trp Leu Leu Leu Ser Leu 
1               5                   10                  15      


Leu Ser Leu Pro Leu Gly Leu Pro Val Leu Gly Ala Pro Pro Arg Leu 
            20                  25                  30          


Ile Cys Asp Ser Arg Val Leu Glu Arg Tyr Leu Leu Glu Ala Lys Glu 
        35                  40                  45              


Ala Glu Asn Ile Thr Thr Gly Cys Ala Glu His Cys Ser Leu Asn Glu 
    50                  55                  60                  


Asn Ile Thr Val Pro Asp Thr Lys Val Asn Phe Tyr Ala Trp Lys Arg 
65                  70                  75                  80  


Met Glu Val Gly Gln Gln Ala Val Glu Val Trp Gln Gly Leu Ala Leu 
                85                  90                  95      


Leu Ser Glu Ala Val Leu Arg Gly Gln Ala Leu Leu Val Asn Ser Ser 
            100                 105                 110         


Gln Pro Trp Glu Pro Leu Gln Leu His Val Asp Lys Ala Val Ser Gly 
        115                 120                 125             


Leu Arg Ser Leu Thr Thr Leu Leu Arg Ala Leu Gly Ala Gln Lys Glu 
    130                 135                 140                 


Ala Ile Ser Pro Pro Asp Ala Ala Ser Ala Ala Pro Leu Arg Thr Ile 
145                 150                 155                 160 


Thr Ala Asp Thr Phe Arg Lys Leu Phe Arg Val Tyr Ser Asn Phe Leu 
                165                 170                 175     


Arg Gly Lys Leu Lys Leu Tyr Thr Gly Glu Ala Cys Arg Thr Gly Asp 
            180                 185                 190         


Arg 
    


<210>  44
<211>  550
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PpLuc

<400>  44

Met Glu Asp Ala Lys Asn Ile Lys Lys Gly Pro Ala Pro Phe Tyr Pro 
1               5                   10                  15      


Leu Glu Asp Gly Thr Ala Gly Glu Gln Leu His Lys Ala Met Lys Arg 
            20                  25                  30          


Tyr Ala Leu Val Pro Gly Thr Ile Ala Phe Thr Asp Ala His Ile Glu 
        35                  40                  45              


Val Asp Ile Thr Tyr Ala Glu Tyr Phe Glu Met Ser Val Arg Leu Ala 
    50                  55                  60                  


Glu Ala Met Lys Arg Tyr Gly Leu Asn Thr Asn His Arg Ile Val Val 
65                  70                  75                  80  


Cys Ser Glu Asn Ser Leu Gln Phe Phe Met Pro Val Leu Gly Ala Leu 
                85                  90                  95      


Phe Ile Gly Val Ala Val Ala Pro Ala Asn Asp Ile Tyr Asn Glu Arg 
            100                 105                 110         


Glu Leu Leu Asn Ser Met Gly Ile Ser Gln Pro Thr Val Val Phe Val 
        115                 120                 125             


Ser Lys Lys Gly Leu Gln Lys Ile Leu Asn Val Gln Lys Lys Leu Pro 
    130                 135                 140                 


Ile Ile Gln Lys Ile Ile Ile Met Asp Ser Lys Thr Asp Tyr Gln Gly 
145                 150                 155                 160 


Phe Gln Ser Met Tyr Thr Phe Val Thr Ser His Leu Pro Pro Gly Phe 
                165                 170                 175     


Asn Glu Tyr Asp Phe Val Pro Glu Ser Phe Asp Arg Asp Lys Thr Ile 
            180                 185                 190         


Ala Leu Ile Met Asn Ser Ser Gly Ser Thr Gly Leu Pro Lys Gly Val 
        195                 200                 205             


Ala Leu Pro His Arg Thr Ala Cys Val Arg Phe Ser His Ala Arg Asp 
    210                 215                 220                 


Pro Ile Phe Gly Asn Gln Ile Ile Pro Asp Thr Ala Ile Leu Ser Val 
225                 230                 235                 240 


Val Pro Phe His His Gly Phe Gly Met Phe Thr Thr Leu Gly Tyr Leu 
                245                 250                 255     


Ile Cys Gly Phe Arg Val Val Leu Met Tyr Arg Phe Glu Glu Glu Leu 
            260                 265                 270         


Phe Leu Arg Ser Leu Gln Asp Tyr Lys Ile Gln Ser Ala Leu Leu Val 
        275                 280                 285             


Pro Thr Leu Phe Ser Phe Phe Ala Lys Ser Thr Leu Ile Asp Lys Tyr 
    290                 295                 300                 


Asp Leu Ser Asn Leu His Glu Ile Ala Ser Gly Gly Ala Pro Leu Ser 
305                 310                 315                 320 


Lys Glu Val Gly Glu Ala Val Ala Lys Arg Phe His Leu Pro Gly Ile 
                325                 330                 335     


Arg Gln Gly Tyr Gly Leu Thr Glu Thr Thr Ser Ala Ile Leu Ile Thr 
            340                 345                 350         


Pro Glu Gly Asp Asp Lys Pro Gly Ala Val Gly Lys Val Val Pro Phe 
        355                 360                 365             


Phe Glu Ala Lys Val Val Asp Leu Asp Thr Gly Lys Thr Leu Gly Val 
    370                 375                 380                 


Asn Gln Arg Gly Glu Leu Cys Val Arg Gly Pro Met Ile Met Ser Gly 
385                 390                 395                 400 


Tyr Val Asn Asn Pro Glu Ala Thr Asn Ala Leu Ile Asp Lys Asp Gly 
                405                 410                 415     


Trp Leu His Ser Gly Asp Ile Ala Tyr Trp Asp Glu Asp Glu His Phe 
            420                 425                 430         


Phe Ile Val Asp Arg Leu Lys Ser Leu Ile Lys Tyr Lys Gly Tyr Gln 
        435                 440                 445             


Val Ala Pro Ala Glu Leu Glu Ser Ile Leu Leu Gln His Pro Asn Ile 
    450                 455                 460                 


Phe Asp Ala Gly Val Ala Gly Leu Pro Asp Asp Asp Ala Gly Glu Leu 
465                 470                 475                 480 


Pro Ala Ala Val Val Val Leu Glu His Gly Lys Thr Met Thr Glu Lys 
                485                 490                 495     


Glu Ile Val Asp Tyr Val Ala Ser Gln Val Thr Thr Ala Lys Lys Leu 
            500                 505                 510         


Arg Gly Gly Val Val Phe Val Asp Glu Val Pro Lys Gly Leu Thr Gly 
        515                 520                 525             


Lys Leu Asp Ala Arg Lys Ile Arg Glu Ile Leu Ile Lys Ala Lys Lys 
    530                 535                 540                 


Gly Gly Lys Ile Ala Val 
545                 550 


<210>  45
<211>  553
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRSV

<400>  45

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 
            100                 105                 110         


Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 
        115                 120                 125             


Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 
    130                 135                 140                 


Gly Ser Ala Ile Ala Ser Gly Val Ala Val Cys Lys Val Leu His Leu 
145                 150                 155                 160 


Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 
                165                 170                 175     


Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val 
            180                 185                 190         


Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn 
        195                 200                 205             


Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln 
    210                 215                 220                 


Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 
225                 230                 235                 240 


Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 
                245                 250                 255     


Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 
            260                 265                 270         


Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 
        275                 280                 285             


Met Cys Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 
    290                 295                 300                 


Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 
305                 310                 315                 320 


Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 
                325                 330                 335     


Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 
            340                 345                 350         


Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 
        355                 360                 365             


Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 
    370                 375                 380                 


Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 
385                 390                 395                 400 


Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 
                405                 410                 415     


Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 
            420                 425                 430         


Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 
        435                 440                 445             


Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 
    450                 455                 460                 


Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro 
465                 470                 475                 480 


Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 
                485                 490                 495     


Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 
            500                 505                 510         


Leu His Asn Val Asn Ala Gly Lys Ser Thr Thr Asn Ile Met Ile Thr 
        515                 520                 525             


Thr Ile Ile Ile Val Ile Ile Val Ile Leu Leu Ser Leu Ile Ala Val 
    530                 535                 540                 


Gly Leu Leu Leu Tyr Cys Lys Ala Arg 
545                 550             


<210>  46
<211>  1575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G

<400>  46
augguuccuc aggcucuccu guuuguaccc cuucugguuu uuccauugug uuuugggaaa       60

uucccuauuu acacgauacc agacaagcuu ggucccugga gcccgauuga cauacaucac      120

cucagcugcc caaacaauuu gguaguggag gacgaaggau gcaccaaccu gucaggguuc      180

uccuacaugg aacuuaaagu uggauacauc ucagccauaa aaaugaacgg guucacuugc      240

acaggcguug ugacggaggc ugaaaccuac acuaacuucg uugguuaugu cacaaccacg      300

uucaaaagaa agcauuuccg cccaacacca gaugcaugua gagccgcgua caacuggaag      360

auggccggug accccagaua ugaagagucu cuacacaauc cguacccuga cuaccacugg      420

cuucgaacug uaaaaaccac caaggagucu cucguuauca uaucuccaag uguggcagau      480

uuggacccau augacagauc ccuucacucg agggucuucc cuggcgggaa uugcucagga      540

guagcggugu cuucuaccua cugcuccacu aaccacgauu acaccauuug gaugcccgag      600

aauccgagac uagggauguc uugugacauu uuuaccaaua guagagggaa gagagcaucc      660

aaagggagug agacuugcgg cuuuguagau gaaagaggcc uauauaaguc uuuaaaagga      720

gcaugcaaac ucaaguuaug uggaguucua ggacuuagac uuauggaugg aacauggguc      780

gcgaugcaaa caucaaauga aaccaaaugg ugcccucccg gucaguuggu gaauuugcac      840

gacuuucgcu cagacgaaau ugagcaccuu guuguagagg aguuggucaa gaagagagag      900

gagugucugg augcacuaga guccaucaug accaccaagu cagugaguuu cagacgucuc      960

agucauuuaa gaaaacuugu cccuggguuu ggaaaagcau auaccauauu caacaagacc     1020

uugauggaag ccgaugcuca cuacaaguca gucagaacuu ggaaugagau caucccuuca     1080

aaaggguguu uaagaguugg ggggaggugu cauccucaug uaaacggggu auuuuucaau     1140

gguauaauau uaggaccuga cggcaauguc uuaaucccag agaugcaauc aucccuccuc     1200

cagcaacaua uggaguuguu gguauccucg guuauccccc uuaugcaccc ccuggcagac     1260

ccgucuaccg uuuucaagaa cggugacgag gcugaggauu uuguugaagu ucaccuuccc     1320

gaugugcacg aacggaucuc aggaguugac uugggucucc cgaacugggg gaaguaugua     1380

uuacugagug caggggcccu gacugccuug auguugauaa uuuuccugau gacaugcugg     1440

agaagaguca aucgaucgga accuacacaa cacaaucuca gagggacagg gagggaggug     1500

ucagucacuc cccaaagcgg gaagaucaua ucuucauggg aaucauacaa gagcgggggu     1560

gagaccggac uguga                                                      1575


<210>  47
<211>  582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO

<400>  47
augggggugc acgaaugucc ugccuggcug uggcuucucc ugucccugcu gucgcucccu       60

cugggccucc caguccuggg cgccccacca cgccucaucu gugacagccg aguccuggag      120

agguaccucu uggaggccaa ggaggccgag aauaucacga cgggcugugc ugaacacugc      180

agcuugaaug agaauaucac ugucccagac accaaaguua auuucuaugc cuggaagagg      240

auggaggucg ggcagcaggc cguagaaguc uggcagggcc uggcccugcu gucggaagcu      300

guccugcggg gccaggcccu guuggucaac ucuucccagc cgugggagcc ccugcagcug      360

cauguggaua aagccgucag uggccuucgc agccucacca cucugcuucg ggcucuggga      420

gcccagaagg aagccaucuc cccuccagau gcggccucag cugcuccacu ccgaacaauc      480

acugcugaca cuuuccgcaa acucuuccga gucuacucca auuuccuccg gggaaagcug      540

aagcuguaca caggggaggc cugcaggaca ggggacagau ga                         582


<210>  48
<211>  1653
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc

<400>  48
auggaagacg ccaaaaacau aaagaaaggc ccggcgccau ucuauccgcu ggaagaugga       60

accgcuggag agcaacugca uaaggcuaug aagagauacg cccugguucc uggaacaauu      120

gcuuuuacag augcacauau cgagguggac aucacuuacg cugaguacuu cgaaaugucc      180

guucgguugg cagaagcuau gaaacgauau gggcugaaua caaaucacag aaucgucgua      240

ugcagugaaa acucucuuca auucuuuaug ccgguguugg gcgcguuauu uaucggaguu      300

gcaguugcgc ccgcgaacga cauuuauaau gaacgugaau ugcucaacag uaugggcauu      360

ucgcagccua ccgugguguu cguuuccaaa aagggguugc aaaaaauuuu gaacgugcaa      420

aaaaagcucc caaucaucca aaaaauuauu aucauggauu cuaaaacgga uuaccaggga      480

uuucagucga uguacacguu cgucacaucu caucuaccuc ccgguuuuaa ugaauacgau      540

uuugugccag aguccuucga uagggacaag acaauugcac ugaucaugaa cuccucugga      600

ucuacugguc ugccuaaagg ugucgcucug ccucauagaa cugccugcgu gagauucucg      660

caugccagag auccuauuuu uggcaaucaa aucauuccgg auacugcgau uuuaaguguu      720

guuccauucc aucacgguuu uggaauguuu acuacacucg gauauuugau auguggauuu      780

cgagucgucu uaauguauag auuugaagaa gagcuguuuc ugaggagccu ucaggauuac      840

aagauucaaa gugcgcugcu ggugccaacc cuauucuccu ucuucgccaa aagcacucug      900

auugacaaau acgauuuauc uaauuuacac gaaauugcuu cugguggcgc uccccucucu      960

aaggaagucg gggaagcggu ugccaagagg uuccaucugc cagguaucag gcaaggauau     1020

gggcucacug agacuacauc agcuauucug auuacacccg agggggauga uaaaccgggc     1080

gcggucggua aaguuguucc auuuuuugaa gcgaagguug uggaucugga uaccgggaaa     1140

acgcugggcg uuaaucaaag aggcgaacug ugugugagag guccuaugau uauguccggu     1200

uauguaaaca auccggaagc gaccaacgcc uugauugaca aggauggaug gcuacauucu     1260

ggagacauag cuuacuggga cgaagacgaa cacuucuuca ucguugaccg ccugaagucu     1320

cugauuaagu acaaaggcua ucagguggcu cccgcugaau uggaauccau cuugcuccaa     1380

caccccaaca ucuucgacgc aggugucgca ggucuucccg acgaugacgc cggugaacuu     1440

cccgccgccg uuguuguuuu ggagcacgga aagacgauga cggaaaaaga gaucguggau     1500

uacgucgcca gucaaguaac aaccgcgaaa aaguugcgcg gaggaguugu guuuguggac     1560

gaaguaccga aaggucuuac cggaaaacuc gacgcaagaa aaaucagaga gauccucaua     1620

aaggccaaga agggcggaaa gaucgccgug uaa                                  1653


<210>  49
<211>  1662
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV

<400>  49
auggaguugc uaauccucaa agcaaaugca auuaccacaa uccucacugc agucacauuu       60

uguuuugcuu cuggucaaaa caucacugaa gaauuuuauc aaucaacaug cagugcaguu      120

agcaaaggcu aucuuagugc ucugagaacu gguugguaua ccaguguuau aacuauagaa      180

uuaaguaaua ucaaggaaaa uaaguguaau ggaacagaug cuaagguaaa auugauaaaa      240

caagaauuag auaaauauaa aaaugcugua acagaauugc aguugcucau gcaaagcaca      300

ccagcaacaa acaaucgagc cagaagagaa cuaccaaggu uuaugaauua uacacucaac      360

aaugccaaaa aaaccaaugu aacauuaagc aagaaaagga aaagaagauu ucuugguuuu      420

uuguuaggug uuggaucugc aaucgccagu ggcguugcug uaugcaaggu ccugcaccua      480

gaaggggaag ugaacaagau caaaagugcu cuacuaucca caaacaaggc uguagucagc      540

uuaucaaaug gaguuagugu cuuaaccuuc aaaguguuag accucaaaaa cuauauagau      600

aaacaauugu uaccuauucu gaacaagcaa agcugcagca uaucaaauau agaaacugug      660

auagaguucc aacaaaagaa caacagacua cuagagauua ccagggaauu uaguguuaau      720

gcagguguaa cuacaccugu aagcacuuac auguuaacua auagugaauu auugucauua      780

aucaaugaua ugccuauaac aaaugaucag aaaaaguuaa uguccaacaa uguucaaaua      840

guuagacagc aaaguuacuc uaucaugugc auaauaaaag aggaagucuu agcauaugua      900

guacaauuac cacuauaugg uguuauagau acacccuguu ggaaacuaca cacauccccu      960

cuauguacaa ccaacacaaa agaagggucc aacaucuguu uaacaagaac ugacagagga     1020

ugguacugug acaaugcagg aucaguaucu uucuucccac aagcugaaac auguaaaguu     1080

caaucaaauc gaguauuuug ugacacaaug aacaguuuaa cauuaccaag ugaaguaaau     1140

cucugcaaug uugacauauu caaccccaaa uaugauugua aaauuaugac uucaaaaaca     1200

gauguaagca gcuccguuau cacaucucua ggagccauug ugucaugcua uggcaaaacu     1260

aaauguacag cauccaauaa aaaucgugga aucauaaaga cauuuucuaa cgggugcgau     1320

uauguaucaa auaaaggggu ggacacugug ucuguaggua acacauuaua uuauguaaau     1380

aagcaagaag guaaaagucu cuauguaaaa ggugaaccaa uaauaaauuu cuaugaccca     1440

uuaguauucc ccucugauga auuugaugca ucaauaucuc aagucaacga gaagauuaac     1500

cagagccuag cauuuauucg uaaauccgau gaauuauuac auaauguaaa ugcugguaaa     1560

uccaccacaa auaucaugau aacuacuaua auuauaguga uuauaguaau auuguuauca     1620

uuaauugcug uuggacugcu cuuauacugu aaggccagau aa                        1662


<210>  50
<211>  1575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G

<400>  50
auggugcccc aggcccuccu guucgucccg cugcuggugu ucccccucug cuucggcaag       60

uuccccaucu acaccauccc cgacaagcug gggccgugga gccccaucga cauccaccac      120

cuguccugcc ccaacaaccu cguggucgag gacgagggcu gcaccaaccu gagcggguuc      180

uccuacaugg agcugaaggu gggcuacauc agcgccauca agaugaacgg guucacgugc      240

accggcgugg ucaccgaggc ggagaccuac acgaacuucg ugggcuacgu gaccaccacc      300

uucaagcgga agcacuuccg ccccacgccg gacgccugcc gggccgccua caacuggaag      360

auggccgggg acccccgcua cgaggagucc cuccacaacc ccuaccccga cuaccacugg      420

cugcggaccg ucaagaccac caaggagagc cuggugauca ucuccccgag cguggcggac      480

cucgaccccu acgaccgcuc ccugcacagc cgggucuucc ccggcgggaa cugcuccggc      540

guggccguga gcuccacgua cugcagcacc aaccacgacu acaccaucug gaugcccgag      600

aacccgcgcc uggggauguc cugcgacauc uucaccaaca gccggggcaa gcgcgccucc      660

aagggcagcg agacgugcgg guucgucgac gagcggggcc ucuacaaguc ccugaagggg      720

gccugcaagc ugaagcucug cggcgugcug ggccugcgcc ucauggacgg gaccugggug      780

gcgaugcaga ccagcaacga gaccaagugg ugcccccccg gccagcuggu caaccugcac      840

gacuuccgga gcgacgagau cgagcaccuc gugguggagg agcuggucaa gaagcgcgag      900

gagugccugg acgcccucga guccaucaug acgaccaaga gcguguccuu ccggcgccug      960

agccaccucc ggaagcuggu gcccggguuc ggcaaggccu acaccaucuu caacaagacc     1020

cugauggagg ccgacgccca cuacaagucc guccgcacgu ggaacgagau caucccgagc     1080

aaggggugcc ugcggguggg cggccgcugc cacccccacg ucaacggggu guucuucaac     1140

ggcaucaucc ucgggcccga cggcaacgug cugauccccg agaugcaguc cagccugcuc     1200

cagcagcaca uggagcugcu ggucuccagc gugaucccgc ucaugcaccc ccuggcggac     1260

cccuccaccg uguucaagaa cggggacgag gccgaggacu ucgucgaggu gcaccucccc     1320

gacgugcacg agcggaucag cggcgucgac cugggccugc cgaacugggg gaaguacgug     1380

cugcucuccg ccggcgcccu gaccgcccug augcugauca ucuuccucau gaccugcugg     1440

cgccggguga accggagcga gcccacgcag cacaaccugc gcgggaccgg ccgggagguc     1500

uccgugaccc cgcagagcgg gaagaucauc uccagcuggg aguccuacaa gagcggcggc     1560

gagaccgggc uguga                                                      1575


<210>  51
<211>  582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO

<400>  51
augggcgugc acgagugccc cgccuggcug uggcuccugc ugagccuccu gucccugccg       60

cucgggcugc ccguccuggg cgcccccccg cggcucaucu gcgacagccg cgugcuggag      120

cgguaccugc ucgaggcgaa ggaggccgag aacaucacca ccgggugcgc cgagcacugc      180

ucccugaacg agaacaucac ggugccggac accaagguca acuucuacgc cuggaagcgc      240

auggaggugg gccagcaggc cguggagguc uggcaggggc uggcgcuccu gagcgaggcc      300

gugcugcggg gccaggcccu ccuggugaac uccagccagc ccugggagcc ccugcagcuc      360

cacgucgaca aggccguguc cggccugcgc agccugacca cccuccugcg ggcgcugggg      420

gcccagaagg aggccaucuc ccccccggac gccgccagcg cggccccgcu ccgcacgauc      480

accgccgaca ccuuccggaa gcuguuccgc guguacucca acuuccugcg gggcaagcuc      540

aagcuguaca ccggggaggc cugccgcacg ggcgaccggu ga                         582


<210>  52
<211>  1653
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc

<400>  52
auggaggacg ccaagaacau caagaagggc ccggcgcccu ucuacccgcu ggaggacggg       60

accgccggcg agcagcucca caaggccaug aagcgguacg cccuggugcc gggcacgauc      120

gccuucaccg acgcccacau cgaggucgac aucaccuacg cggaguacuu cgagaugagc      180

gugcgccugg ccgaggccau gaagcgguac ggccugaaca ccaaccaccg gaucguggug      240

ugcucggaga acagccugca guucuucaug ccggugcugg gcgcccucuu caucggcgug      300

gccgucgccc cggcgaacga caucuacaac gagcgggagc ugcugaacag cauggggauc      360

agccagccga ccgugguguu cgugagcaag aagggccugc agaagauccu gaacgugcag      420

aagaagcugc ccaucaucca gaagaucauc aucauggaca gcaagaccga cuaccagggc      480

uuccagucga uguacacguu cgugaccagc caccucccgc cgggcuucaa cgaguacgac      540

uucgucccgg agagcuucga ccgggacaag accaucgccc ugaucaugaa cagcagcggc      600

agcaccggcc ugccgaaggg gguggcccug ccgcaccgga ccgccugcgu gcgcuucucg      660

cacgcccggg accccaucuu cggcaaccag aucaucccgg acaccgccau ccugagcgug      720

gugccguucc accacggcuu cggcauguuc acgacccugg gcuaccucau cugcggcuuc      780

cggguggucc ugauguaccg guucgaggag gagcuguucc ugcggagccu gcaggacuac      840

aagauccaga gcgcgcugcu cgugccgacc cuguucagcu ucuucgccaa gagcacccug      900

aucgacaagu acgaccuguc gaaccugcac gagaucgcca gcgggggcgc cccgcugagc      960

aaggaggugg gcgaggccgu ggccaagcgg uuccaccucc cgggcauccg ccagggcuac     1020

ggccugaccg agaccacgag cgcgauccug aucacccccg agggggacga caagccgggc     1080

gccgugggca aggugguccc guucuucgag gccaaggugg uggaccugga caccggcaag     1140

acccugggcg ugaaccagcg gggcgagcug ugcgugcggg ggccgaugau caugagcggc     1200

uacgugaaca acccggaggc caccaacgcc cucaucgaca aggacggcug gcugcacagc     1260

ggcgacaucg ccuacuggga cgaggacgag cacuucuuca ucgucgaccg gcugaagucg     1320

cugaucaagu acaagggcua ccagguggcg ccggccgagc uggagagcau ccugcuccag     1380

caccccaaca ucuucgacgc cggcguggcc gggcugccgg acgacgacgc cggcgagcug     1440

ccggccgcgg ugguggugcu ggagcacggc aagaccauga cggagaagga gaucgucgac     1500

uacguggcca gccaggugac caccgccaag aagcugcggg gcggcguggu guucguggac     1560

gaggucccga agggccugac cgggaagcuc gacgcccgga agauccgcga gauccugauc     1620

aaggccaaga agggcggcaa gaucgccgug uga                                  1653


<210>  53
<211>  1662
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV

<400>  53
auggagcugc ucauccugaa ggccaacgcc aucaccacca uccugacggc ggugaccuuc       60

ugcuucgcca gcggccagaa caucaccgag gaguucuacc aguccacgug cagcgccguc      120

uccaaggggu accucagcgc ccugcggacc ggcugguaca ccuccgugau caccaucgag      180

cugagcaaca ucaaggagaa caagugcaac gggaccgacg ccaaggugaa gcucaucaag      240

caggagcugg acaaguacaa gaacgcgguc acggagcugc agcuccugau gcaguccacc      300

ccggcaacca acaaccgcgc ccggcgcgag cugccccggu ucaugaacua cacccucaac      360

aacgccaaga agacgaacgu gacccugagc aagaagcgca agcggcgcuu ccugggcuuc      420

cuccuggggg ugggcuccgc caucgcgagc ggcgucgccg ugugcaaggu gcugcaccuc      480

gagggggagg ucaacaagau caagagcgcc cugcugucca ccaacaaggc cguggugagc      540

cucuccaacg gcgucagcgu gcugaccuuc aaggugcugg accucaagaa cuacaucgac      600

aagcagcugc ugcccauccu gaacaagcag agcugcucca ucagcaacau cgagacggug      660

aucgaguucc agcagaagaa caaccggcuc cuggagauca cccgcgaguu cagcgugaac      720

gccgggguca ccacccccgu guccacguac augcugacca acagcgagcu ccugucccug      780

aucaacgaca ugccgaucac caacgaccag aagaagcuca ugagcaacaa cgugcagauc      840

guccggcagc aguccuacag caucaugugc aucaucaagg aggaggugcu ggcguacgug      900

guccagcugc cccucuacgg cgugaucgac acccccugcu ggaagcugca cacgagcccc      960

cugugcacca ccaacaccaa ggaggggucc aacaucugcc ucacgcgcac cgaccggggc     1020

ugguacugcg acaacgccgg cagcgucucc uucuucccgc aggccgagac cugcaaggug     1080

cagagcaacc gcguguucug cgacaccaug aacucccuga cgcugcccag cgagguaaac     1140

cucugcaacg ucgacaucuu caaccccaag uacgacugca agaucaugac cuccaagacc     1200

gacgugagcu ccagcgugau caccucccug ggggccaucg ucagcugcua cggcaagacg     1260

aagugcaccg ccagcaacaa gaaccggggg aucaucaaga ccuucuccaa cggcugcgac     1320

uacgugagca acaagggcgu ggacaccgug uccgucggga acacccugua cuacgugaac     1380

aagcaggagg gcaagagccu cuacgugaag ggggagccca ucaucaacuu cuacgacccg     1440

cuggucuucc ccuccgacga guucgacgcg agcaucuccc aggugaacga gaagaucaac     1500

cagagccugg ccuucauccg caaguccgac gagcugcucc acaacgugaa cgccggcaag     1560

agcacgacca acaucaugau caccaccauc aucaucguca ucaucgugau ccugcugucc     1620

cucaucgccg uggggcugcu gcucuacugc aaggcccggu ga                        1662


<210>  54
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  54
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu cccuguuccc agagcccacu     1680

uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc     1800

cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau u              1851


<210>  55
<211>  1792
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  55
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu cccuguuccc agagcccacu     1680

uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             1792


<210>  56
<211>  858
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  56
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc      720

uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu      840

uucagagcca ccagaauu                                                    858


<210>  57
<211>  799
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  57
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc      720

uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaagaauu                                                   799


<210>  58
<211>  1929
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  58
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuagucc     1740

cuguucccag agcccacuuu uuuuucuuuu uuugaaauaa aauagccugu cuuucagauc     1800

uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu uuucagagcc     1920

accagaauu                                                             1929


<210>  59
<211>  1870
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  59
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuagucc     1740

cuguucccag agcccacuuu uuuuucuuuu uuugaaauaa aauagccugu cuuucagauc     1800

uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaagaauu                                                            1870


<210>  60
<211>  1938
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  60
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gagcugcuca uccugaaggc caacgccauc accaccaucc      120

ugacggcggu gaccuucugc uucgccagcg gccagaacau caccgaggag uucuaccagu      180

ccacgugcag cgccgucucc aagggguacc ucagcgcccu gcggaccggc ugguacaccu      240

ccgugaucac caucgagcug agcaacauca aggagaacaa gugcaacggg accgacgcca      300

aggugaagcu caucaagcag gagcuggaca aguacaagaa cgcggucacg gagcugcagc      360

uccugaugca guccaccccg gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca      420

ugaacuacac ccucaacaac gccaagaaga cgaacgugac ccugagcaag aagcgcaagc      480

ggcgcuuccu gggcuuccuc cugggggugg gcuccgccau cgcgagcggc gucgccgugu      540

gcaaggugcu gcaccucgag ggggagguca acaagaucaa gagcgcccug cuguccacca      600

acaaggccgu ggugagccuc uccaacggcg ucagcgugcu gaccuucaag gugcuggacc      660

ucaagaacua caucgacaag cagcugcugc ccauccugaa caagcagagc ugcuccauca      720

gcaacaucga gacggugauc gaguuccagc agaagaacaa ccggcuccug gagaucaccc      780

gcgaguucag cgugaacgcc ggggucacca cccccguguc cacguacaug cugaccaaca      840

gcgagcuccu gucccugauc aacgacaugc cgaucaccaa cgaccagaag aagcucauga      900

gcaacaacgu gcagaucguc cggcagcagu ccuacagcau caugugcauc aucaaggagg      960

aggugcuggc guacgugguc cagcugcccc ucuacggcgu gaucgacacc cccugcugga     1020

agcugcacac gagcccccug ugcaccacca acaccaagga gggguccaac aucugccuca     1080

cgcgcaccga ccggggcugg uacugcgaca acgccggcag cgucuccuuc uucccgcagg     1140

ccgagaccug caaggugcag agcaaccgcg uguucugcga caccaugaac ucccugacgc     1200

ugcccagcga gguaaaccuc ugcaacgucg acaucuucaa ccccaaguac gacugcaaga     1260

ucaugaccuc caagaccgac gugagcucca gcgugaucac cucccugggg gccaucguca     1320

gcugcuacgg caagacgaag ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu     1380

ucuccaacgg cugcgacuac gugagcaaca agggcgugga caccgugucc gucgggaaca     1440

cccuguacua cgugaacaag caggagggca agagccucua cgugaagggg gagcccauca     1500

ucaacuucua cgacccgcug gucuuccccu ccgacgaguu cgacgcgagc aucucccagg     1560

ugaacgagaa gaucaaccag agccuggccu ucauccgcaa guccgacgag cugcuccaca     1620

acgugaacgc cggcaagagc acgaccaaca ucaugaucac caccaucauc aucgucauca     1680

ucgugauccu gcugucccuc aucgccgugg ggcugcugcu cuacugcaag gcccggugag     1740

gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc     1800

uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu     1920

uucagagcca ccagaauu                                                   1938


<210>  61
<211>  1915
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  61
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu aauaaggaaa cuguaugaau     1680

gucugugggc aggaagugaa gagauccuuc uguaaagguu uuuggaauua ugucugcuga     1740

auaauaaacu uuuuugaaau aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     1860

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          1915


<210>  62
<211>  1856
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  62
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu aauaaggaaa cuguaugaau     1680

gucugugggc aggaagugaa gagauccuuc uguaaagguu uuuggaauua ugucugcuga     1740

auaauaaacu uuuuugaaau aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         1856


<210>  63
<211>  922
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  63
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag auccuucugu      720

aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau aaaucuggua      780

gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc      900

ucuuuucaga gccaccagaa uu                                               922


<210>  64
<211>  863
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  64
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag auccuucugu      720

aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau aaaucuggua      780

gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaga auu                                              863


<210>  65
<211>  1993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  65
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuaguaa     1740

uaaggaaacu guaugaaugu cugugggcag gaagugaaga gauccuucug uaaagguuuu     1800

uggaauuaug ucugcugaau aauaaacuuu uuugaaauaa uaaaucuggu agaaaaauga     1860

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1980

agccaccaga auu                                                        1993


<210>  66
<211>  1934
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  66
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuaguaa     1740

uaaggaaacu guaugaaugu cugugggcag gaagugaaga gauccuucug uaaagguuuu     1800

uggaauuaug ucugcugaau aauaaacuuu uuugaaauaa uaaaucuggu agaaaaauga     1860

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaag aauu                                                       1934


<210>  67
<211>  2002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  67
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gagcugcuca uccugaaggc caacgccauc accaccaucc      120

ugacggcggu gaccuucugc uucgccagcg gccagaacau caccgaggag uucuaccagu      180

ccacgugcag cgccgucucc aagggguacc ucagcgcccu gcggaccggc ugguacaccu      240

ccgugaucac caucgagcug agcaacauca aggagaacaa gugcaacggg accgacgcca      300

aggugaagcu caucaagcag gagcuggaca aguacaagaa cgcggucacg gagcugcagc      360

uccugaugca guccaccccg gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca      420

ugaacuacac ccucaacaac gccaagaaga cgaacgugac ccugagcaag aagcgcaagc      480

ggcgcuuccu gggcuuccuc cugggggugg gcuccgccau cgcgagcggc gucgccgugu      540

gcaaggugcu gcaccucgag ggggagguca acaagaucaa gagcgcccug cuguccacca      600

acaaggccgu ggugagccuc uccaacggcg ucagcgugcu gaccuucaag gugcuggacc      660

ucaagaacua caucgacaag cagcugcugc ccauccugaa caagcagagc ugcuccauca      720

gcaacaucga gacggugauc gaguuccagc agaagaacaa ccggcuccug gagaucaccc      780

gcgaguucag cgugaacgcc ggggucacca cccccguguc cacguacaug cugaccaaca      840

gcgagcuccu gucccugauc aacgacaugc cgaucaccaa cgaccagaag aagcucauga      900

gcaacaacgu gcagaucguc cggcagcagu ccuacagcau caugugcauc aucaaggagg      960

aggugcuggc guacgugguc cagcugcccc ucuacggcgu gaucgacacc cccugcugga     1020

agcugcacac gagcccccug ugcaccacca acaccaagga gggguccaac aucugccuca     1080

cgcgcaccga ccggggcugg uacugcgaca acgccggcag cgucuccuuc uucccgcagg     1140

ccgagaccug caaggugcag agcaaccgcg uguucugcga caccaugaac ucccugacgc     1200

ugcccagcga gguaaaccuc ugcaacgucg acaucuucaa ccccaaguac gacugcaaga     1260

ucaugaccuc caagaccgac gugagcucca gcgugaucac cucccugggg gccaucguca     1320

gcugcuacgg caagacgaag ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu     1380

ucuccaacgg cugcgacuac gugagcaaca agggcgugga caccgugucc gucgggaaca     1440

cccuguacua cgugaacaag caggagggca agagccucua cgugaagggg gagcccauca     1500

ucaacuucua cgacccgcug gucuuccccu ccgacgaguu cgacgcgagc aucucccagg     1560

ugaacgagaa gaucaaccag agccuggccu ucauccgcaa guccgacgag cugcuccaca     1620

acgugaacgc cggcaagagc acgaccaaca ucaugaucac caccaucauc aucgucauca     1680

ucgugauccu gcugucccuc aucgccgugg ggcugcugcu cuacugcaag gcccggugag     1740

gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag auccuucugu     1800

aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau aaaucuggua     1860

gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1980

ucuuuucaga gccaccagaa uu                                              2002


<210>  68
<211>  1931
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  68
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu acuggcugca ucucccuuuc     1680

cucuguccuc cauccuucuc ccaggauggu gaagggggac cugguaccca gugaucccca     1740

ccccaggauc cuaaaucaug acuuaccugc uaauaaaaac ucauuggaaa agugagaaga     1800

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag     1920

ccaccagaau u                                                          1931


<210>  69
<211>  1872
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  69
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu acuggcugca ucucccuuuc     1680

cucuguccuc cauccuucuc ccaggauggu gaagggggac cugguaccca gugaucccca     1740

ccccaggauc cuaaaucaug acuuaccugc uaauaaaaac ucauuggaaa agugagaaga     1800

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaagaa uu                                                         1872


<210>  70
<211>  938
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  70
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuaguacu ggcugcaucu cccuuuccuc uguccuccau ccuucuccca ggauggugaa      720

gggggaccug guacccagug auccccaccc caggauccua aaucaugacu uaccugcuaa      780

uaaaaacuca uuggaaaagu gagaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc      900

cccccccccc aaaggcucuu uucagagcca ccagaauu                              938


<210>  71
<211>  879
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  71
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuaguacu ggcugcaucu cccuuuccuc uguccuccau ccuucuccca ggauggugaa      720

gggggaccug guacccagug auccccaccc caggauccua aaucaugacu uaccugcuaa      780

uaaaaacuca uuggaaaagu gagaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                             879


<210>  72
<211>  2009
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  72
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuaguac     1740

uggcugcauc ucccuuuccu cuguccucca uccuucuccc aggaugguga agggggaccu     1800

gguacccagu gauccccacc ccaggauccu aaaucaugac uuaccugcua auaaaaacuc     1860

auuggaaaag ugagaagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc     1980

caaaggcucu uuucagagcc accagaauu                                       2009


<210>  73
<211>  1950
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  73
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuaguac     1740

uggcugcauc ucccuuuccu cuguccucca uccuucuccc aggaugguga agggggaccu     1800

gguacccagu gauccccacc ccaggauccu aaaucaugac uuaccugcua auaaaaacuc     1860

auuggaaaag ugagaagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      1950


<210>  74
<211>  2018
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  74
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gagcugcuca uccugaaggc caacgccauc accaccaucc      120

ugacggcggu gaccuucugc uucgccagcg gccagaacau caccgaggag uucuaccagu      180

ccacgugcag cgccgucucc aagggguacc ucagcgcccu gcggaccggc ugguacaccu      240

ccgugaucac caucgagcug agcaacauca aggagaacaa gugcaacggg accgacgcca      300

aggugaagcu caucaagcag gagcuggaca aguacaagaa cgcggucacg gagcugcagc      360

uccugaugca guccaccccg gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca      420

ugaacuacac ccucaacaac gccaagaaga cgaacgugac ccugagcaag aagcgcaagc      480

ggcgcuuccu gggcuuccuc cugggggugg gcuccgccau cgcgagcggc gucgccgugu      540

gcaaggugcu gcaccucgag ggggagguca acaagaucaa gagcgcccug cuguccacca      600

acaaggccgu ggugagccuc uccaacggcg ucagcgugcu gaccuucaag gugcuggacc      660

ucaagaacua caucgacaag cagcugcugc ccauccugaa caagcagagc ugcuccauca      720

gcaacaucga gacggugauc gaguuccagc agaagaacaa ccggcuccug gagaucaccc      780

gcgaguucag cgugaacgcc ggggucacca cccccguguc cacguacaug cugaccaaca      840

gcgagcuccu gucccugauc aacgacaugc cgaucaccaa cgaccagaag aagcucauga      900

gcaacaacgu gcagaucguc cggcagcagu ccuacagcau caugugcauc aucaaggagg      960

aggugcuggc guacgugguc cagcugcccc ucuacggcgu gaucgacacc cccugcugga     1020

agcugcacac gagcccccug ugcaccacca acaccaagga gggguccaac aucugccuca     1080

cgcgcaccga ccggggcugg uacugcgaca acgccggcag cgucuccuuc uucccgcagg     1140

ccgagaccug caaggugcag agcaaccgcg uguucugcga caccaugaac ucccugacgc     1200

ugcccagcga gguaaaccuc ugcaacgucg acaucuucaa ccccaaguac gacugcaaga     1260

ucaugaccuc caagaccgac gugagcucca gcgugaucac cucccugggg gccaucguca     1320

gcugcuacgg caagacgaag ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu     1380

ucuccaacgg cugcgacuac gugagcaaca agggcgugga caccgugucc gucgggaaca     1440

cccuguacua cgugaacaag caggagggca agagccucua cgugaagggg gagcccauca     1500

ucaacuucua cgacccgcug gucuuccccu ccgacgaguu cgacgcgagc aucucccagg     1560

ugaacgagaa gaucaaccag agccuggccu ucauccgcaa guccgacgag cugcuccaca     1620

acgugaacgc cggcaagagc acgaccaaca ucaugaucac caccaucauc aucgucauca     1680

ucgugauccu gcugucccuc aucgccgugg ggcugcugcu cuacugcaag gcccggugag     1740

gacuaguacu ggcugcaucu cccuuuccuc uguccuccau ccuucuccca ggauggugaa     1800

gggggaccug guacccagug auccccaccc caggauccua aaucaugacu uaccugcuaa     1860

uaaaaacuca uuggaaaagu gagaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc     1980

cccccccccc aaaggcucuu uucagagcca ccagaauu                             2018


<210>  75
<211>  2147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  75
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu gaagggaaca cccaaauuua     1680

auucagccuu aagcacaauu aauuaagagu gaaacguaau uguacaagca guuggucacc     1740

caccauaggg caugaucaac accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac     1800

cccucuuccc uucagcuugc uuagauguuc caaauuuagu aagcuuaagg cggccuacag     1860

aagaaaaaga aaaaaaaggc cacaaaaguu cccucucacu uucaguaaau aaaauaaaag     1920

cagcaacaga aauaaagaaa uaaaugaaau ucaaaaugaa auaaauauug uguugugcag     1980

cauuaaaaaa ucaauaaaaa uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc     2100

cccccccccc ccccccccca aaggcucuuu ucagagccac cagaauu                   2147


<210>  76
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  76
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu gaagggaaca cccaaauuua     1680

auucagccuu aagcacaauu aauuaagagu gaaacguaau uguacaagca guuggucacc     1740

caccauaggg caugaucaac accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac     1800

cccucuuccc uucagcuugc uuagauguuc caaauuuagu aagcuuaagg cggccuacag     1860

aagaaaaaga aaaaaaaggc cacaaaaguu cccucucacu uucaguaaau aaaauaaaag     1920

cagcaacaga aauaaagaaa uaaaugaaau ucaaaaugaa auaaauauug uguugugcag     1980

cauuaaaaaa ucaauaaaaa uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  2088


<210>  77
<211>  1154
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  77
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau uaagagugaa      720

acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc gcaaccuuuc      780

cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa      840

auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc      900

ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa augaaauuca      960

aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua aaaaugagca     1020

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca     1140

gagccaccag aauu                                                       1154


<210>  78
<211>  1095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  78
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau uaagagugaa      720

acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc gcaaccuuuc      780

cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa      840

auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc      900

ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa augaaauuca      960

aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua aaaaugagca     1020

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa gaauu                                                      1095


<210>  79
<211>  2225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  79
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuaguga     1740

agggaacacc caaauuuaau ucagccuuaa gcacaauuaa uuaagaguga aacguaauug     1800

uacaagcagu uggucaccca ccauagggca ugaucaacac cgcaaccuuu ccuuuuuccc     1860

ccagugauuc ugaaaaaccc cucuucccuu cagcuugcuu agauguucca aauuuaguaa     1920

gcuuaaggcg gccuacagaa gaaaaagaaa aaaaaggcca caaaaguucc cucucacuuu     1980

caguaaauaa aauaaaagca gcaacagaaa uaaagaaaua aaugaaauuc aaaaugaaau     2040

aaauauugug uugugcagca uuaaaaaauc aauaaaaauu aaaaaugagc aagaucuaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

augcaucccc cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca     2220

gaauu                                                                 2225


<210>  80
<211>  2166
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  80
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuaguga     1740

agggaacacc caaauuuaau ucagccuuaa gcacaauuaa uuaagaguga aacguaauug     1800

uacaagcagu uggucaccca ccauagggca ugaucaacac cgcaaccuuu ccuuuuuccc     1860

ccagugauuc ugaaaaaccc cucuucccuu cagcuugcuu agauguucca aauuuaguaa     1920

gcuuaaggcg gccuacagaa gaaaaagaaa aaaaaggcca caaaaguucc cucucacuuu     1980

caguaaauaa aauaaaagca gcaacagaaa uaaagaaaua aaugaaauuc aaaaugaaau     2040

aaauauugug uugugcagca uuaaaaaauc aauaaaaauu aaaaaugagc aagaucuaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

agaauu                                                                2166


<210>  81
<211>  2234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  81
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gagcugcuca uccugaaggc caacgccauc accaccaucc      120

ugacggcggu gaccuucugc uucgccagcg gccagaacau caccgaggag uucuaccagu      180

ccacgugcag cgccgucucc aagggguacc ucagcgcccu gcggaccggc ugguacaccu      240

ccgugaucac caucgagcug agcaacauca aggagaacaa gugcaacggg accgacgcca      300

aggugaagcu caucaagcag gagcuggaca aguacaagaa cgcggucacg gagcugcagc      360

uccugaugca guccaccccg gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca      420

ugaacuacac ccucaacaac gccaagaaga cgaacgugac ccugagcaag aagcgcaagc      480

ggcgcuuccu gggcuuccuc cugggggugg gcuccgccau cgcgagcggc gucgccgugu      540

gcaaggugcu gcaccucgag ggggagguca acaagaucaa gagcgcccug cuguccacca      600

acaaggccgu ggugagccuc uccaacggcg ucagcgugcu gaccuucaag gugcuggacc      660

ucaagaacua caucgacaag cagcugcugc ccauccugaa caagcagagc ugcuccauca      720

gcaacaucga gacggugauc gaguuccagc agaagaacaa ccggcuccug gagaucaccc      780

gcgaguucag cgugaacgcc ggggucacca cccccguguc cacguacaug cugaccaaca      840

gcgagcuccu gucccugauc aacgacaugc cgaucaccaa cgaccagaag aagcucauga      900

gcaacaacgu gcagaucguc cggcagcagu ccuacagcau caugugcauc aucaaggagg      960

aggugcuggc guacgugguc cagcugcccc ucuacggcgu gaucgacacc cccugcugga     1020

agcugcacac gagcccccug ugcaccacca acaccaagga gggguccaac aucugccuca     1080

cgcgcaccga ccggggcugg uacugcgaca acgccggcag cgucuccuuc uucccgcagg     1140

ccgagaccug caaggugcag agcaaccgcg uguucugcga caccaugaac ucccugacgc     1200

ugcccagcga gguaaaccuc ugcaacgucg acaucuucaa ccccaaguac gacugcaaga     1260

ucaugaccuc caagaccgac gugagcucca gcgugaucac cucccugggg gccaucguca     1320

gcugcuacgg caagacgaag ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu     1380

ucuccaacgg cugcgacuac gugagcaaca agggcgugga caccgugucc gucgggaaca     1440

cccuguacua cgugaacaag caggagggca agagccucua cgugaagggg gagcccauca     1500

ucaacuucua cgacccgcug gucuuccccu ccgacgaguu cgacgcgagc aucucccagg     1560

ugaacgagaa gaucaaccag agccuggccu ucauccgcaa guccgacgag cugcuccaca     1620

acgugaacgc cggcaagagc acgaccaaca ucaugaucac caccaucauc aucgucauca     1680

ucgugauccu gcugucccuc aucgccgugg ggcugcugcu cuacugcaag gcccggugag     1740

gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau uaagagugaa     1800

acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc gcaaccuuuc     1860

cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa     1920

auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc     1980

ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa augaaauuca     2040

aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua aaaaugagca     2100

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca     2220

gagccaccag aauu                                                       2234


<210>  82
<211>  1927
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  82
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu ggaagcauuu uccuggcuga     1680

uuaaaagaaa uuacucagcu auggucaucu guuccuguua gaaggcuaug cagcauauua     1740

uauacuaugc gcauguuaug aaaugcauaa uaaaaaauuu uaaaaaaucu aaaagaucua     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     1920

cagaauu                                                               1927


<210>  83
<211>  1868
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  83
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu ggaagcauuu uccuggcuga     1680

uuaaaagaaa uuacucagcu auggucaucu guuccuguua gaaggcuaug cagcauauua     1740

uauacuaugc gcauguuaug aaaugcauaa uaaaaaauuu uaaaaaaucu aaaagaucua     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaagaauu                                                              1868


<210>  84
<211>  934
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  84
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuagugga agcauuuucc uggcugauua aaagaaauua cucagcuaug gucaucuguu      720

ccuguuagaa ggcuaugcag cauauuauau acuaugcgca uguuaugaaa ugcauaauaa      780

aaaauuuuaa aaaaucuaaa agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc      900

ccccccaaag gcucuuuuca gagccaccag aauu                                  934


<210>  85
<211>  875
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  85
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuagugga agcauuuucc uggcugauua aaagaaauua cucagcuaug gucaucuguu      720

ccuguuagaa ggcuaugcag cauauuauau acuaugcgca uguuaugaaa ugcauaauaa      780

aaaauuuuaa aaaaucuaaa agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                 875


<210>  86
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  86
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuagugg     1740

aagcauuuuc cuggcugauu aaaagaaauu acucagcuau ggucaucugu uccuguuaga     1800

aggcuaugca gcauauuaua uacuaugcgc auguuaugaa augcauaaua aaaaauuuua     1860

aaaaaucuaa aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  87
<211>  1946
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  87
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuagugg     1740

aagcauuuuc cuggcugauu aaaagaaauu acucagcuau ggucaucugu uccuguuaga     1800

aggcuaugca gcauauuaua uacuaugcgc auguuaugaa augcauaaua aaaaauuuua     1860

aaaaaucuaa aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa agaauu                                          1946


<210>  88
<211>  2014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  88
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gagcugcuca uccugaaggc caacgccauc accaccaucc      120

ugacggcggu gaccuucugc uucgccagcg gccagaacau caccgaggag uucuaccagu      180

ccacgugcag cgccgucucc aagggguacc ucagcgcccu gcggaccggc ugguacaccu      240

ccgugaucac caucgagcug agcaacauca aggagaacaa gugcaacggg accgacgcca      300

aggugaagcu caucaagcag gagcuggaca aguacaagaa cgcggucacg gagcugcagc      360

uccugaugca guccaccccg gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca      420

ugaacuacac ccucaacaac gccaagaaga cgaacgugac ccugagcaag aagcgcaagc      480

ggcgcuuccu gggcuuccuc cugggggugg gcuccgccau cgcgagcggc gucgccgugu      540

gcaaggugcu gcaccucgag ggggagguca acaagaucaa gagcgcccug cuguccacca      600

acaaggccgu ggugagccuc uccaacggcg ucagcgugcu gaccuucaag gugcuggacc      660

ucaagaacua caucgacaag cagcugcugc ccauccugaa caagcagagc ugcuccauca      720

gcaacaucga gacggugauc gaguuccagc agaagaacaa ccggcuccug gagaucaccc      780

gcgaguucag cgugaacgcc ggggucacca cccccguguc cacguacaug cugaccaaca      840

gcgagcuccu gucccugauc aacgacaugc cgaucaccaa cgaccagaag aagcucauga      900

gcaacaacgu gcagaucguc cggcagcagu ccuacagcau caugugcauc aucaaggagg      960

aggugcuggc guacgugguc cagcugcccc ucuacggcgu gaucgacacc cccugcugga     1020

agcugcacac gagcccccug ugcaccacca acaccaagga gggguccaac aucugccuca     1080

cgcgcaccga ccggggcugg uacugcgaca acgccggcag cgucuccuuc uucccgcagg     1140

ccgagaccug caaggugcag agcaaccgcg uguucugcga caccaugaac ucccugacgc     1200

ugcccagcga gguaaaccuc ugcaacgucg acaucuucaa ccccaaguac gacugcaaga     1260

ucaugaccuc caagaccgac gugagcucca gcgugaucac cucccugggg gccaucguca     1320

gcugcuacgg caagacgaag ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu     1380

ucuccaacgg cugcgacuac gugagcaaca agggcgugga caccgugucc gucgggaaca     1440

cccuguacua cgugaacaag caggagggca agagccucua cgugaagggg gagcccauca     1500

ucaacuucua cgacccgcug gucuuccccu ccgacgaguu cgacgcgagc aucucccagg     1560

ugaacgagaa gaucaaccag agccuggccu ucauccgcaa guccgacgag cugcuccaca     1620

acgugaacgc cggcaagagc acgaccaaca ucaugaucac caccaucauc aucgucauca     1680

ucgugauccu gcugucccuc aucgccgugg ggcugcugcu cuacugcaag gcccggugag     1740

gacuagugga agcauuuucc uggcugauua aaagaaauua cucagcuaug gucaucuguu     1800

ccuguuagaa ggcuaugcag cauauuauau acuaugcgca uguuaugaaa ugcauaauaa     1860

aaaauuuuaa aaaaucuaaa agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     1980

ccccccaaag gcucuuuuca gagccaccag aauu                                 2014


<210>  89
<211>  1864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  89
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu guccaccugu cccuccuggg     1680

cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1860

aauu                                                                  1864


<210>  90
<211>  1805
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  90
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gugccccagg cccuccuguu cgucccgcug cugguguucc      120

cccucugcuu cggcaaguuc cccaucuaca ccauccccga caagcugggg ccguggagcc      180

ccaucgacau ccaccaccug uccugcccca acaaccucgu ggucgaggac gagggcugca      240

ccaaccugag cggguucucc uacauggagc ugaagguggg cuacaucagc gccaucaaga      300

ugaacggguu cacgugcacc ggcgugguca ccgaggcgga gaccuacacg aacuucgugg      360

gcuacgugac caccaccuuc aagcggaagc acuuccgccc cacgccggac gccugccggg      420

ccgccuacaa cuggaagaug gccggggacc cccgcuacga ggagucccuc cacaaccccu      480

accccgacua ccacuggcug cggaccguca agaccaccaa ggagagccug gugaucaucu      540

ccccgagcgu ggcggaccuc gaccccuacg accgcucccu gcacagccgg gucuuccccg      600

gcgggaacug cuccggcgug gccgugagcu ccacguacug cagcaccaac cacgacuaca      660

ccaucuggau gcccgagaac ccgcgccugg ggauguccug cgacaucuuc accaacagcc      720

ggggcaagcg cgccuccaag ggcagcgaga cgugcggguu cgucgacgag cggggccucu      780

acaagucccu gaagggggcc ugcaagcuga agcucugcgg cgugcugggc cugcgccuca      840

uggacgggac cuggguggcg augcagacca gcaacgagac caaguggugc ccccccggcc      900

agcuggucaa ccugcacgac uuccggagcg acgagaucga gcaccucgug guggaggagc      960

uggucaagaa gcgcgaggag ugccuggacg cccucgaguc caucaugacg accaagagcg     1020

uguccuuccg gcgccugagc caccuccgga agcuggugcc cggguucggc aaggccuaca     1080

ccaucuucaa caagacccug auggaggccg acgcccacua caaguccguc cgcacgugga     1140

acgagaucau cccgagcaag gggugccugc gggugggcgg ccgcugccac ccccacguca     1200

acgggguguu cuucaacggc aucauccucg ggcccgacgg caacgugcug auccccgaga     1260

ugcaguccag ccugcuccag cagcacaugg agcugcuggu cuccagcgug aucccgcuca     1320

ugcacccccu ggcggacccc uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg     1380

ucgaggugca ccuccccgac gugcacgagc ggaucagcgg cgucgaccug ggccugccga     1440

acugggggaa guacgugcug cucuccgccg gcgcccugac cgcccugaug cugaucaucu     1500

uccucaugac cugcuggcgc cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg     1560

ggaccggccg ggaggucucc gugaccccgc agagcgggaa gaucaucucc agcugggagu     1620

ccuacaagag cggcggcgag accgggcugu gaggacuagu guccaccugu cccuccuggg     1680

cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

gaauu                                                                 1805


<210>  91
<211>  871
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  91
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu gccaaauaaa      720

caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc      840

cccaaaggcu cuuuucagag ccaccagaau u                                     871


<210>  92
<211>  812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  92
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug ggcgugcacg agugccccgc cuggcugugg cuccugcuga      120

gccuccuguc ccugccgcuc gggcugcccg uccugggcgc ccccccgcgg cucaucugcg      180

acagccgcgu gcuggagcgg uaccugcucg aggcgaagga ggccgagaac aucaccaccg      240

ggugcgccga gcacugcucc cugaacgaga acaucacggu gccggacacc aaggucaacu      300

ucuacgccug gaagcgcaug gaggugggcc agcaggccgu ggaggucugg caggggcugg      360

cgcuccugag cgaggccgug cugcggggcc aggcccuccu ggugaacucc agccagcccu      420

gggagccccu gcagcuccac gucgacaagg ccguguccgg ccugcgcagc cugaccaccc      480

uccugcgggc gcugggggcc cagaaggagg ccaucucccc cccggacgcc gccagcgcgg      540

ccccgcuccg cacgaucacc gccgacaccu uccggaagcu guuccgcgug uacuccaacu      600

uccugcgggg caagcucaag cuguacaccg gggaggccug ccgcacgggc gaccggugag      660

gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu gccaaauaaa      720

caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                    812


<210>  93
<211>  1942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  93
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuagugu     1740

ccaccugucc cuccugggcu gcuggauugu cucguuuucc ugccaaauaa acaggaucag     1800

cgcuuuacag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1920

ucuuuucaga gccaccagaa uu                                              1942


<210>  94
<211>  1883
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  94
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gaggacgcca agaacaucaa gaagggcccg gcgcccuucu      120

acccgcugga ggacgggacc gccggcgagc agcuccacaa ggccaugaag cgguacgccc      180

uggugccggg cacgaucgcc uucaccgacg cccacaucga ggucgacauc accuacgcgg      240

aguacuucga gaugagcgug cgccuggccg aggccaugaa gcgguacggc cugaacacca      300

accaccggau cguggugugc ucggagaaca gccugcaguu cuucaugccg gugcugggcg      360

cccucuucau cggcguggcc gucgccccgg cgaacgacau cuacaacgag cgggagcugc      420

ugaacagcau ggggaucagc cagccgaccg ugguguucgu gagcaagaag ggccugcaga      480

agauccugaa cgugcagaag aagcugccca ucauccagaa gaucaucauc auggacagca      540

agaccgacua ccagggcuuc cagucgaugu acacguucgu gaccagccac cucccgccgg      600

gcuucaacga guacgacuuc gucccggaga gcuucgaccg ggacaagacc aucgcccuga      660

ucaugaacag cagcggcagc accggccugc cgaagggggu ggcccugccg caccggaccg      720

ccugcgugcg cuucucgcac gcccgggacc ccaucuucgg caaccagauc aucccggaca      780

ccgccauccu gagcguggug ccguuccacc acggcuucgg cauguucacg acccugggcu      840

accucaucug cggcuuccgg gugguccuga uguaccgguu cgaggaggag cuguuccugc      900

ggagccugca ggacuacaag auccagagcg cgcugcucgu gccgacccug uucagcuucu      960

ucgccaagag cacccugauc gacaaguacg accugucgaa ccugcacgag aucgccagcg     1020

ggggcgcccc gcugagcaag gaggugggcg aggccguggc caagcgguuc caccucccgg     1080

gcauccgcca gggcuacggc cugaccgaga ccacgagcgc gauccugauc acccccgagg     1140

gggacgacaa gccgggcgcc gugggcaagg uggucccguu cuucgaggcc aagguggugg     1200

accuggacac cggcaagacc cugggcguga accagcgggg cgagcugugc gugcgggggc     1260

cgaugaucau gagcggcuac gugaacaacc cggaggccac caacgcccuc aucgacaagg     1320

acggcuggcu gcacagcggc gacaucgccu acugggacga ggacgagcac uucuucaucg     1380

ucgaccggcu gaagucgcug aucaaguaca agggcuacca gguggcgccg gccgagcugg     1440

agagcauccu gcuccagcac cccaacaucu ucgacgccgg cguggccggg cugccggacg     1500

acgacgccgg cgagcugccg gccgcggugg uggugcugga gcacggcaag accaugacgg     1560

agaaggagau cgucgacuac guggccagcc aggugaccac cgccaagaag cugcggggcg     1620

gcgugguguu cguggacgag gucccgaagg gccugaccgg gaagcucgac gcccggaaga     1680

uccgcgagau ccugaucaag gccaagaagg gcggcaagau cgccguguga ggacuagugu     1740

ccaccugucc cuccugggcu gcuggauugu cucguuuucc ugccaaauaa acaggaucag     1800

cgcuuuacag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaga auu                                             1883


<210>  95
<211>  1951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  95
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gagcugcuca uccugaaggc caacgccauc accaccaucc      120

ugacggcggu gaccuucugc uucgccagcg gccagaacau caccgaggag uucuaccagu      180

ccacgugcag cgccgucucc aagggguacc ucagcgcccu gcggaccggc ugguacaccu      240

ccgugaucac caucgagcug agcaacauca aggagaacaa gugcaacggg accgacgcca      300

aggugaagcu caucaagcag gagcuggaca aguacaagaa cgcggucacg gagcugcagc      360

uccugaugca guccaccccg gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca      420

ugaacuacac ccucaacaac gccaagaaga cgaacgugac ccugagcaag aagcgcaagc      480

ggcgcuuccu gggcuuccuc cugggggugg gcuccgccau cgcgagcggc gucgccgugu      540

gcaaggugcu gcaccucgag ggggagguca acaagaucaa gagcgcccug cuguccacca      600

acaaggccgu ggugagccuc uccaacggcg ucagcgugcu gaccuucaag gugcuggacc      660

ucaagaacua caucgacaag cagcugcugc ccauccugaa caagcagagc ugcuccauca      720

gcaacaucga gacggugauc gaguuccagc agaagaacaa ccggcuccug gagaucaccc      780

gcgaguucag cgugaacgcc ggggucacca cccccguguc cacguacaug cugaccaaca      840

gcgagcuccu gucccugauc aacgacaugc cgaucaccaa cgaccagaag aagcucauga      900

gcaacaacgu gcagaucguc cggcagcagu ccuacagcau caugugcauc aucaaggagg      960

aggugcuggc guacgugguc cagcugcccc ucuacggcgu gaucgacacc cccugcugga     1020

agcugcacac gagcccccug ugcaccacca acaccaagga gggguccaac aucugccuca     1080

cgcgcaccga ccggggcugg uacugcgaca acgccggcag cgucuccuuc uucccgcagg     1140

ccgagaccug caaggugcag agcaaccgcg uguucugcga caccaugaac ucccugacgc     1200

ugcccagcga gguaaaccuc ugcaacgucg acaucuucaa ccccaaguac gacugcaaga     1260

ucaugaccuc caagaccgac gugagcucca gcgugaucac cucccugggg gccaucguca     1320

gcugcuacgg caagacgaag ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu     1380

ucuccaacgg cugcgacuac gugagcaaca agggcgugga caccgugucc gucgggaaca     1440

cccuguacua cgugaacaag caggagggca agagccucua cgugaagggg gagcccauca     1500

ucaacuucua cgacccgcug gucuuccccu ccgacgaguu cgacgcgagc aucucccagg     1560

ugaacgagaa gaucaaccag agccuggccu ucauccgcaa guccgacgag cugcuccaca     1620

acgugaacgc cggcaagagc acgaccaaca ucaugaucac caccaucauc aucgucauca     1680

ucgugauccu gcugucccuc aucgccgugg ggcugcugcu cuacugcaag gcccggugag     1740

gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu gccaaauaaa     1800

caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1920

cccaaaggcu cuuuucagag ccaccagaau u                                    1951


<210>  96
<211>  1844
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  96
gggagagccu cugcuggagu ccggggagug gcguuggcug cuagagcgaa gcuuaccaug       60

gugccccagg cccuccuguu cgucccgcug cugguguucc cccucugcuu cggcaaguuc      120

cccaucuaca ccauccccga caagcugggg ccguggagcc ccaucgacau ccaccaccug      180

uccugcccca acaaccucgu ggucgaggac gagggcugca ccaaccugag cggguucucc      240

uacauggagc ugaagguggg cuacaucagc gccaucaaga ugaacggguu cacgugcacc      300

ggcgugguca ccgaggcgga gaccuacacg aacuucgugg gcuacgugac caccaccuuc      360

aagcggaagc acuuccgccc cacgccggac gccugccggg ccgccuacaa cuggaagaug      420

gccggggacc cccgcuacga ggagucccuc cacaaccccu accccgacua ccacuggcug      480

cggaccguca agaccaccaa ggagagccug gugaucaucu ccccgagcgu ggcggaccuc      540

gaccccuacg accgcucccu gcacagccgg gucuuccccg gcgggaacug cuccggcgug      600

gccgugagcu ccacguacug cagcaccaac cacgacuaca ccaucuggau gcccgagaac      660

ccgcgccugg ggauguccug cgacaucuuc accaacagcc ggggcaagcg cgccuccaag      720

ggcagcgaga cgugcggguu cgucgacgag cggggccucu acaagucccu gaagggggcc      780

ugcaagcuga agcucugcgg cgugcugggc cugcgccuca uggacgggac cuggguggcg      840

augcagacca gcaacgagac caaguggugc ccccccggcc agcuggucaa ccugcacgac      900

uuccggagcg acgagaucga gcaccucgug guggaggagc uggucaagaa gcgcgaggag      960

ugccuggacg cccucgaguc caucaugacg accaagagcg uguccuuccg gcgccugagc     1020

caccuccgga agcuggugcc cggguucggc aaggccuaca ccaucuucaa caagacccug     1080

auggaggccg acgcccacua caaguccguc cgcacgugga acgagaucau cccgagcaag     1140

gggugccugc gggugggcgg ccgcugccac ccccacguca acgggguguu cuucaacggc     1200

aucauccucg ggcccgacgg caacgugcug auccccgaga ugcaguccag ccugcuccag     1260

cagcacaugg agcugcuggu cuccagcgug aucccgcuca ugcacccccu ggcggacccc     1320

uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg ucgaggugca ccuccccgac     1380

gugcacgagc ggaucagcgg cgucgaccug ggccugccga acugggggaa guacgugcug     1440

cucuccgccg gcgcccugac cgcccugaug cugaucaucu uccucaugac cugcuggcgc     1500

cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg ggaccggccg ggaggucucc     1560

gugaccccgc agagcgggaa gaucaucucc agcugggagu ccuacaagag cggcggcgag     1620

accgggcugu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu     1680

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc     1800

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                      1844


<210>  97
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  97
gggagagccu cugcuggagu ccggggagug gcguuggcug cuagagcgaa gcuuaccaug       60

gugccccagg cccuccuguu cgucccgcug cugguguucc cccucugcuu cggcaaguuc      120

cccaucuaca ccauccccga caagcugggg ccguggagcc ccaucgacau ccaccaccug      180

uccugcccca acaaccucgu ggucgaggac gagggcugca ccaaccugag cggguucucc      240

uacauggagc ugaagguggg cuacaucagc gccaucaaga ugaacggguu cacgugcacc      300

ggcgugguca ccgaggcgga gaccuacacg aacuucgugg gcuacgugac caccaccuuc      360

aagcggaagc acuuccgccc cacgccggac gccugccggg ccgccuacaa cuggaagaug      420

gccggggacc cccgcuacga ggagucccuc cacaaccccu accccgacua ccacuggcug      480

cggaccguca agaccaccaa ggagagccug gugaucaucu ccccgagcgu ggcggaccuc      540

gaccccuacg accgcucccu gcacagccgg gucuuccccg gcgggaacug cuccggcgug      600

gccgugagcu ccacguacug cagcaccaac cacgacuaca ccaucuggau gcccgagaac      660

ccgcgccugg ggauguccug cgacaucuuc accaacagcc ggggcaagcg cgccuccaag      720

ggcagcgaga cgugcggguu cgucgacgag cggggccucu acaagucccu gaagggggcc      780

ugcaagcuga agcucugcgg cgugcugggc cugcgccuca uggacgggac cuggguggcg      840

augcagacca gcaacgagac caaguggugc ccccccggcc agcuggucaa ccugcacgac      900

uuccggagcg acgagaucga gcaccucgug guggaggagc uggucaagaa gcgcgaggag      960

ugccuggacg cccucgaguc caucaugacg accaagagcg uguccuuccg gcgccugagc     1020

caccuccgga agcuggugcc cggguucggc aaggccuaca ccaucuucaa caagacccug     1080

auggaggccg acgcccacua caaguccguc cgcacgugga acgagaucau cccgagcaag     1140

gggugccugc gggugggcgg ccgcugccac ccccacguca acgggguguu cuucaacggc     1200

aucauccucg ggcccgacgg caacgugcug auccccgaga ugcaguccag ccugcuccag     1260

cagcacaugg agcugcuggu cuccagcgug aucccgcuca ugcacccccu ggcggacccc     1320

uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg ucgaggugca ccuccccgac     1380

gugcacgagc ggaucagcgg cgucgaccug ggccugccga acugggggaa guacgugcug     1440

cucuccgccg gcgcccugac cgcccugaug cugaucaucu uccucaugac cugcuggcgc     1500

cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg ggaccggccg ggaggucucc     1560

gugaccccgc agagcgggaa gaucaucucc agcugggagu ccuacaagag cggcggcgag     1620

accgggcugu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu     1680

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     1785


<210>  98
<211>  851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  98
gggagagccu cugcuggagu ccggggagug gcguuggcug cuagagcgaa gcuuaccaug       60

ggcgugcacg agugccccgc cuggcugugg cuccugcuga gccuccuguc ccugccgcuc      120

gggcugcccg uccugggcgc ccccccgcgg cucaucugcg acagccgcgu gcuggagcgg      180

uaccugcucg aggcgaagga ggccgagaac aucaccaccg ggugcgccga gcacugcucc      240

cugaacgaga acaucacggu gccggacacc aaggucaacu ucuacgccug gaagcgcaug      300

gaggugggcc agcaggccgu ggaggucugg caggggcugg cgcuccugag cgaggccgug      360

cugcggggcc aggcccuccu ggugaacucc agccagcccu gggagccccu gcagcuccac      420

gucgacaagg ccguguccgg ccugcgcagc cugaccaccc uccugcgggc gcugggggcc      480

cagaaggagg ccaucucccc cccggacgcc gccagcgcgg ccccgcuccg cacgaucacc      540

gccgacaccu uccggaagcu guuccgcgug uacuccaacu uccugcgggg caagcucaag      600

cuguacaccg gggaggccug ccgcacgggc gaccggugag gacuaguguc caccuguccc      660

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga      720

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag      840

ccaccagaau u                                                           851


<210>  99
<211>  792
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  99
gggagagccu cugcuggagu ccggggagug gcguuggcug cuagagcgaa gcuuaccaug       60

ggcgugcacg agugccccgc cuggcugugg cuccugcuga gccuccuguc ccugccgcuc      120

gggcugcccg uccugggcgc ccccccgcgg cucaucugcg acagccgcgu gcuggagcgg      180

uaccugcucg aggcgaagga ggccgagaac aucaccaccg ggugcgccga gcacugcucc      240

cugaacgaga acaucacggu gccggacacc aaggucaacu ucuacgccug gaagcgcaug      300

gaggugggcc agcaggccgu ggaggucugg caggggcugg cgcuccugag cgaggccgug      360

cugcggggcc aggcccuccu ggugaacucc agccagcccu gggagccccu gcagcuccac      420

gucgacaagg ccguguccgg ccugcgcagc cugaccaccc uccugcgggc gcugggggcc      480

cagaaggagg ccaucucccc cccggacgcc gccagcgcgg ccccgcuccg cacgaucacc      540

gccgacaccu uccggaagcu guuccgcgug uacuccaacu uccugcgggg caagcucaag      600

cuguacaccg gggaggccug ccgcacgggc gaccggugag gacuaguguc caccuguccc      660

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga      720

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaagaa uu                                                          792


<210>  100
<211>  1922
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  100
gggagagccu cugcuggagu ccggggagug gcguuggcug cuagagcgaa gcuuaccaug       60

gaggacgcca agaacaucaa gaagggcccg gcgcccuucu acccgcugga ggacgggacc      120

gccggcgagc agcuccacaa ggccaugaag cgguacgccc uggugccggg cacgaucgcc      180

uucaccgacg cccacaucga ggucgacauc accuacgcgg aguacuucga gaugagcgug      240

cgccuggccg aggccaugaa gcgguacggc cugaacacca accaccggau cguggugugc      300

ucggagaaca gccugcaguu cuucaugccg gugcugggcg cccucuucau cggcguggcc      360

gucgccccgg cgaacgacau cuacaacgag cgggagcugc ugaacagcau ggggaucagc      420

cagccgaccg ugguguucgu gagcaagaag ggccugcaga agauccugaa cgugcagaag      480

aagcugccca ucauccagaa gaucaucauc auggacagca agaccgacua ccagggcuuc      540

cagucgaugu acacguucgu gaccagccac cucccgccgg gcuucaacga guacgacuuc      600

gucccggaga gcuucgaccg ggacaagacc aucgcccuga ucaugaacag cagcggcagc      660

accggccugc cgaagggggu ggcccugccg caccggaccg ccugcgugcg cuucucgcac      720

gcccgggacc ccaucuucgg caaccagauc aucccggaca ccgccauccu gagcguggug      780

ccguuccacc acggcuucgg cauguucacg acccugggcu accucaucug cggcuuccgg      840

gugguccuga uguaccgguu cgaggaggag cuguuccugc ggagccugca ggacuacaag      900

auccagagcg cgcugcucgu gccgacccug uucagcuucu ucgccaagag cacccugauc      960

gacaaguacg accugucgaa ccugcacgag aucgccagcg ggggcgcccc gcugagcaag     1020

gaggugggcg aggccguggc caagcgguuc caccucccgg gcauccgcca gggcuacggc     1080

cugaccgaga ccacgagcgc gauccugauc acccccgagg gggacgacaa gccgggcgcc     1140

gugggcaagg uggucccguu cuucgaggcc aagguggugg accuggacac cggcaagacc     1200

cugggcguga accagcgggg cgagcugugc gugcgggggc cgaugaucau gagcggcuac     1260

gugaacaacc cggaggccac caacgcccuc aucgacaagg acggcuggcu gcacagcggc     1320

gacaucgccu acugggacga ggacgagcac uucuucaucg ucgaccggcu gaagucgcug     1380

aucaaguaca agggcuacca gguggcgccg gccgagcugg agagcauccu gcuccagcac     1440

cccaacaucu ucgacgccgg cguggccggg cugccggacg acgacgccgg cgagcugccg     1500

gccgcggugg uggugcugga gcacggcaag accaugacgg agaaggagau cgucgacuac     1560

guggccagcc aggugaccac cgccaagaag cugcggggcg gcgugguguu cguggacgag     1620

gucccgaagg gccugaccgg gaagcucgac gcccggaaga uccgcgagau ccugaucaag     1680

gccaagaagg gcggcaagau cgccguguga ggacuagugu ccaccugucc cuccugggcu     1740

gcuggauugu cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug     1860

cauccccccc cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa     1920

uu                                                                    1922


<210>  101
<211>  1863
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  101
gggagagccu cugcuggagu ccggggagug gcguuggcug cuagagcgaa gcuuaccaug       60

gaggacgcca agaacaucaa gaagggcccg gcgcccuucu acccgcugga ggacgggacc      120

gccggcgagc agcuccacaa ggccaugaag cgguacgccc uggugccggg cacgaucgcc      180

uucaccgacg cccacaucga ggucgacauc accuacgcgg aguacuucga gaugagcgug      240

cgccuggccg aggccaugaa gcgguacggc cugaacacca accaccggau cguggugugc      300

ucggagaaca gccugcaguu cuucaugccg gugcugggcg cccucuucau cggcguggcc      360

gucgccccgg cgaacgacau cuacaacgag cgggagcugc ugaacagcau ggggaucagc      420

cagccgaccg ugguguucgu gagcaagaag ggccugcaga agauccugaa cgugcagaag      480

aagcugccca ucauccagaa gaucaucauc auggacagca agaccgacua ccagggcuuc      540

cagucgaugu acacguucgu gaccagccac cucccgccgg gcuucaacga guacgacuuc      600

gucccggaga gcuucgaccg ggacaagacc aucgcccuga ucaugaacag cagcggcagc      660

accggccugc cgaagggggu ggcccugccg caccggaccg ccugcgugcg cuucucgcac      720

gcccgggacc ccaucuucgg caaccagauc aucccggaca ccgccauccu gagcguggug      780

ccguuccacc acggcuucgg cauguucacg acccugggcu accucaucug cggcuuccgg      840

gugguccuga uguaccgguu cgaggaggag cuguuccugc ggagccugca ggacuacaag      900

auccagagcg cgcugcucgu gccgacccug uucagcuucu ucgccaagag cacccugauc      960

gacaaguacg accugucgaa ccugcacgag aucgccagcg ggggcgcccc gcugagcaag     1020

gaggugggcg aggccguggc caagcgguuc caccucccgg gcauccgcca gggcuacggc     1080

cugaccgaga ccacgagcgc gauccugauc acccccgagg gggacgacaa gccgggcgcc     1140

gugggcaagg uggucccguu cuucgaggcc aagguggugg accuggacac cggcaagacc     1200

cugggcguga accagcgggg cgagcugugc gugcgggggc cgaugaucau gagcggcuac     1260

gugaacaacc cggaggccac caacgcccuc aucgacaagg acggcuggcu gcacagcggc     1320

gacaucgccu acugggacga ggacgagcac uucuucaucg ucgaccggcu gaagucgcug     1380

aucaaguaca agggcuacca gguggcgccg gccgagcugg agagcauccu gcuccagcac     1440

cccaacaucu ucgacgccgg cguggccggg cugccggacg acgacgccgg cgagcugccg     1500

gccgcggugg uggugcugga gcacggcaag accaugacgg agaaggagau cgucgacuac     1560

guggccagcc aggugaccac cgccaagaag cugcggggcg gcgugguguu cguggacgag     1620

gucccgaagg gccugaccgg gaagcucgac gcccggaaga uccgcgagau ccugaucaag     1680

gccaagaagg gcggcaagau cgccguguga ggacuagugu ccaccugucc cuccugggcu     1740

gcuggauugu cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     1860

auu                                                                   1863


<210>  102
<211>  1931
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  102
gggagagccu cugcuggagu ccggggagug gcguuggcug cuagagcgaa gcuuaccaug       60

gagcugcuca uccugaaggc caacgccauc accaccaucc ugacggcggu gaccuucugc      120

uucgccagcg gccagaacau caccgaggag uucuaccagu ccacgugcag cgccgucucc      180

aagggguacc ucagcgcccu gcggaccggc ugguacaccu ccgugaucac caucgagcug      240

agcaacauca aggagaacaa gugcaacggg accgacgcca aggugaagcu caucaagcag      300

gagcuggaca aguacaagaa cgcggucacg gagcugcagc uccugaugca guccaccccg      360

gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca ugaacuacac ccucaacaac      420

gccaagaaga cgaacgugac ccugagcaag aagcgcaagc ggcgcuuccu gggcuuccuc      480

cugggggugg gcuccgccau cgcgagcggc gucgccgugu gcaaggugcu gcaccucgag      540

ggggagguca acaagaucaa gagcgcccug cuguccacca acaaggccgu ggugagccuc      600

uccaacggcg ucagcgugcu gaccuucaag gugcuggacc ucaagaacua caucgacaag      660

cagcugcugc ccauccugaa caagcagagc ugcuccauca gcaacaucga gacggugauc      720

gaguuccagc agaagaacaa ccggcuccug gagaucaccc gcgaguucag cgugaacgcc      780

ggggucacca cccccguguc cacguacaug cugaccaaca gcgagcuccu gucccugauc      840

aacgacaugc cgaucaccaa cgaccagaag aagcucauga gcaacaacgu gcagaucguc      900

cggcagcagu ccuacagcau caugugcauc aucaaggagg aggugcuggc guacgugguc      960

cagcugcccc ucuacggcgu gaucgacacc cccugcugga agcugcacac gagcccccug     1020

ugcaccacca acaccaagga gggguccaac aucugccuca cgcgcaccga ccggggcugg     1080

uacugcgaca acgccggcag cgucuccuuc uucccgcagg ccgagaccug caaggugcag     1140

agcaaccgcg uguucugcga caccaugaac ucccugacgc ugcccagcga gguaaaccuc     1200

ugcaacgucg acaucuucaa ccccaaguac gacugcaaga ucaugaccuc caagaccgac     1260

gugagcucca gcgugaucac cucccugggg gccaucguca gcugcuacgg caagacgaag     1320

ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu ucuccaacgg cugcgacuac     1380

gugagcaaca agggcgugga caccgugucc gucgggaaca cccuguacua cgugaacaag     1440

caggagggca agagccucua cgugaagggg gagcccauca ucaacuucua cgacccgcug     1500

gucuuccccu ccgacgaguu cgacgcgagc aucucccagg ugaacgagaa gaucaaccag     1560

agccuggccu ucauccgcaa guccgacgag cugcuccaca acgugaacgc cggcaagagc     1620

acgaccaaca ucaugaucac caccaucauc aucgucauca ucgugauccu gcugucccuc     1680

aucgccgugg ggcugcugcu cuacugcaag gcccggugag gacuaguguc caccuguccc     1740

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     1800

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag     1920

ccaccagaau u                                                          1931


<210>  103
<211>  1864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  103
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu agucccuguu     1680

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1860

aauu                                                                  1864


<210>  104
<211>  1805
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  104
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu agucccuguu     1680

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

gaauu                                                                 1805


<210>  105
<211>  871
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  105
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau      720

aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc      840

cccaaaggcu cuuuucagag ccaccagaau u                                     871


<210>  106
<211>  812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  106
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau      720

aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                    812


<210>  107
<211>  1942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  107
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag ucccuguucc cagagcccac uuuuuuuucu uuuuuugaaa uaaaauagcc     1800

ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1920

ucuuuucaga gccaccagaa uu                                              1942


<210>  108
<211>  1883
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  108
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag ucccuguucc cagagcccac uuuuuuuucu uuuuuugaaa uaaaauagcc     1800

ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaga auu                                             1883


<210>  109
<211>  1951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  109
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggagcugc ucauccugaa ggccaacgcc      120

aucaccacca uccugacggc ggugaccuuc ugcuucgcca gcggccagaa caucaccgag      180

gaguucuacc aguccacgug cagcgccguc uccaaggggu accucagcgc ccugcggacc      240

ggcugguaca ccuccgugau caccaucgag cugagcaaca ucaaggagaa caagugcaac      300

gggaccgacg ccaaggugaa gcucaucaag caggagcugg acaaguacaa gaacgcgguc      360

acggagcugc agcuccugau gcaguccacc ccggcaacca acaaccgcgc ccggcgcgag      420

cugccccggu ucaugaacua cacccucaac aacgccaaga agacgaacgu gacccugagc      480

aagaagcgca agcggcgcuu ccugggcuuc cuccuggggg ugggcuccgc caucgcgagc      540

ggcgucgccg ugugcaaggu gcugcaccuc gagggggagg ucaacaagau caagagcgcc      600

cugcugucca ccaacaaggc cguggugagc cucuccaacg gcgucagcgu gcugaccuuc      660

aaggugcugg accucaagaa cuacaucgac aagcagcugc ugcccauccu gaacaagcag      720

agcugcucca ucagcaacau cgagacggug aucgaguucc agcagaagaa caaccggcuc      780

cuggagauca cccgcgaguu cagcgugaac gccgggguca ccacccccgu guccacguac      840

augcugacca acagcgagcu ccugucccug aucaacgaca ugccgaucac caacgaccag      900

aagaagcuca ugagcaacaa cgugcagauc guccggcagc aguccuacag caucaugugc      960

aucaucaagg aggaggugcu ggcguacgug guccagcugc cccucuacgg cgugaucgac     1020

acccccugcu ggaagcugca cacgagcccc cugugcacca ccaacaccaa ggaggggucc     1080

aacaucugcc ucacgcgcac cgaccggggc ugguacugcg acaacgccgg cagcgucucc     1140

uucuucccgc aggccgagac cugcaaggug cagagcaacc gcguguucug cgacaccaug     1200

aacucccuga cgcugcccag cgagguaaac cucugcaacg ucgacaucuu caaccccaag     1260

uacgacugca agaucaugac cuccaagacc gacgugagcu ccagcgugau caccucccug     1320

ggggccaucg ucagcugcua cggcaagacg aagugcaccg ccagcaacaa gaaccggggg     1380

aucaucaaga ccuucuccaa cggcugcgac uacgugagca acaagggcgu ggacaccgug     1440

uccgucggga acacccugua cuacgugaac aagcaggagg gcaagagccu cuacgugaag     1500

ggggagccca ucaucaacuu cuacgacccg cuggucuucc ccuccgacga guucgacgcg     1560

agcaucuccc aggugaacga gaagaucaac cagagccugg ccuucauccg caaguccgac     1620

gagcugcucc acaacgugaa cgccggcaag agcacgacca acaucaugau caccaccauc     1680

aucaucguca ucaucgugau ccugcugucc cucaucgccg uggggcugcu gcucuacugc     1740

aaggcccggu gaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau     1800

aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1920

cccaaaggcu cuuuucagag ccaccagaau u                                    1951


<210>  110
<211>  1928
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  110
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu aguaauaagg     1680

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa     1740

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca     1920

ccagaauu                                                              1928


<210>  111
<211>  1869
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  111
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu aguaauaagg     1680

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa     1740

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaagaauu                                                             1869


<210>  112
<211>  935
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  112
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa      720

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau      780

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc      900

cccccccaaa ggcucuuuuc agagccacca gaauu                                 935


<210>  113
<211>  876
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  113
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa      720

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau      780

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                                876


<210>  114
<211>  2006
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  114
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag uaauaaggaa acuguaugaa ugucuguggg caggaaguga agagauccuu     1800

cuguaaaggu uuuuggaauu augucugcug aauaauaaac uuuuuugaaa uaauaaaucu     1860

gguagaaaaa ugagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa     1980

aggcucuuuu cagagccacc agaauu                                          2006


<210>  115
<211>  1947
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  115
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag uaauaaggaa acuguaugaa ugucuguggg caggaaguga agagauccuu     1800

cuguaaaggu uuuuggaauu augucugcug aauaauaaac uuuuuugaaa uaauaaaucu     1860

gguagaaaaa ugagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aagaauu                                         1947


<210>  116
<211>  2015
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  116
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggagcugc ucauccugaa ggccaacgcc      120

aucaccacca uccugacggc ggugaccuuc ugcuucgcca gcggccagaa caucaccgag      180

gaguucuacc aguccacgug cagcgccguc uccaaggggu accucagcgc ccugcggacc      240

ggcugguaca ccuccgugau caccaucgag cugagcaaca ucaaggagaa caagugcaac      300

gggaccgacg ccaaggugaa gcucaucaag caggagcugg acaaguacaa gaacgcgguc      360

acggagcugc agcuccugau gcaguccacc ccggcaacca acaaccgcgc ccggcgcgag      420

cugccccggu ucaugaacua cacccucaac aacgccaaga agacgaacgu gacccugagc      480

aagaagcgca agcggcgcuu ccugggcuuc cuccuggggg ugggcuccgc caucgcgagc      540

ggcgucgccg ugugcaaggu gcugcaccuc gagggggagg ucaacaagau caagagcgcc      600

cugcugucca ccaacaaggc cguggugagc cucuccaacg gcgucagcgu gcugaccuuc      660

aaggugcugg accucaagaa cuacaucgac aagcagcugc ugcccauccu gaacaagcag      720

agcugcucca ucagcaacau cgagacggug aucgaguucc agcagaagaa caaccggcuc      780

cuggagauca cccgcgaguu cagcgugaac gccgggguca ccacccccgu guccacguac      840

augcugacca acagcgagcu ccugucccug aucaacgaca ugccgaucac caacgaccag      900

aagaagcuca ugagcaacaa cgugcagauc guccggcagc aguccuacag caucaugugc      960

aucaucaagg aggaggugcu ggcguacgug guccagcugc cccucuacgg cgugaucgac     1020

acccccugcu ggaagcugca cacgagcccc cugugcacca ccaacaccaa ggaggggucc     1080

aacaucugcc ucacgcgcac cgaccggggc ugguacugcg acaacgccgg cagcgucucc     1140

uucuucccgc aggccgagac cugcaaggug cagagcaacc gcguguucug cgacaccaug     1200

aacucccuga cgcugcccag cgagguaaac cucugcaacg ucgacaucuu caaccccaag     1260

uacgacugca agaucaugac cuccaagacc gacgugagcu ccagcgugau caccucccug     1320

ggggccaucg ucagcugcua cggcaagacg aagugcaccg ccagcaacaa gaaccggggg     1380

aucaucaaga ccuucuccaa cggcugcgac uacgugagca acaagggcgu ggacaccgug     1440

uccgucggga acacccugua cuacgugaac aagcaggagg gcaagagccu cuacgugaag     1500

ggggagccca ucaucaacuu cuacgacccg cuggucuucc ccuccgacga guucgacgcg     1560

agcaucuccc aggugaacga gaagaucaac cagagccugg ccuucauccg caaguccgac     1620

gagcugcucc acaacgugaa cgccggcaag agcacgacca acaucaugau caccaccauc     1680

aucaucguca ucaucgugau ccugcugucc cucaucgccg uggggcugcu gcucuacugc     1740

aaggcccggu gaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     1800

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     1860

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc     1980

cccccccaaa ggcucuuuuc agagccacca gaauu                                2015


<210>  117
<211>  1944
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  117
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu aguacuggcu     1680

gcaucucccu uuccucuguc cuccauccuu cucccaggau ggugaagggg gaccugguac     1740

ccagugaucc ccaccccagg auccuaaauc augacuuacc ugcuaauaaa aacucauugg     1800

aaaagugaga agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag     1920

gcucuuuuca gagccaccag aauu                                            1944


<210>  118
<211>  1885
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  118
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu aguacuggcu     1680

gcaucucccu uuccucuguc cuccauccuu cucccaggau ggugaagggg gaccugguac     1740

ccagugaucc ccaccccagg auccuaaauc augacuuacc ugcuaauaaa aacucauugg     1800

aaaagugaga agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa gaauu                                           1885


<210>  119
<211>  951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  119
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu acuggcugca ucucccuuuc cucuguccuc cauccuucuc      720

ccaggauggu gaagggggac cugguaccca gugaucccca ccccaggauc cuaaaucaug      780

acuuaccugc uaauaaaaac ucauuggaaa agugagaaga ucuaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc      900

cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau u               951


<210>  120
<211>  892
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  120
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu acuggcugca ucucccuuuc cucuguccuc cauccuucuc      720

ccaggauggu gaagggggac cugguaccca gugaucccca ccccaggauc cuaaaucaug      780

acuuaccugc uaauaaaaac ucauuggaaa agugagaaga ucuaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu              892


<210>  121
<211>  2022
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  121
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag uacuggcugc aucucccuuu ccucuguccu ccauccuucu cccaggaugg     1800

ugaaggggga ccugguaccc agugaucccc accccaggau ccuaaaucau gacuuaccug     1860

cuaauaaaaa cucauuggaa aagugagaag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc     1980

cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu                        2022


<210>  122
<211>  1963
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  122
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag uacuggcugc aucucccuuu ccucuguccu ccauccuucu cccaggaugg     1800

ugaaggggga ccugguaccc agugaucccc accccaggau ccuaaaucau gacuuaccug     1860

cuaauaaaaa cucauuggaa aagugagaag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                       1963


<210>  123
<211>  2031
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  123
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggagcugc ucauccugaa ggccaacgcc      120

aucaccacca uccugacggc ggugaccuuc ugcuucgcca gcggccagaa caucaccgag      180

gaguucuacc aguccacgug cagcgccguc uccaaggggu accucagcgc ccugcggacc      240

ggcugguaca ccuccgugau caccaucgag cugagcaaca ucaaggagaa caagugcaac      300

gggaccgacg ccaaggugaa gcucaucaag caggagcugg acaaguacaa gaacgcgguc      360

acggagcugc agcuccugau gcaguccacc ccggcaacca acaaccgcgc ccggcgcgag      420

cugccccggu ucaugaacua cacccucaac aacgccaaga agacgaacgu gacccugagc      480

aagaagcgca agcggcgcuu ccugggcuuc cuccuggggg ugggcuccgc caucgcgagc      540

ggcgucgccg ugugcaaggu gcugcaccuc gagggggagg ucaacaagau caagagcgcc      600

cugcugucca ccaacaaggc cguggugagc cucuccaacg gcgucagcgu gcugaccuuc      660

aaggugcugg accucaagaa cuacaucgac aagcagcugc ugcccauccu gaacaagcag      720

agcugcucca ucagcaacau cgagacggug aucgaguucc agcagaagaa caaccggcuc      780

cuggagauca cccgcgaguu cagcgugaac gccgggguca ccacccccgu guccacguac      840

augcugacca acagcgagcu ccugucccug aucaacgaca ugccgaucac caacgaccag      900

aagaagcuca ugagcaacaa cgugcagauc guccggcagc aguccuacag caucaugugc      960

aucaucaagg aggaggugcu ggcguacgug guccagcugc cccucuacgg cgugaucgac     1020

acccccugcu ggaagcugca cacgagcccc cugugcacca ccaacaccaa ggaggggucc     1080

aacaucugcc ucacgcgcac cgaccggggc ugguacugcg acaacgccgg cagcgucucc     1140

uucuucccgc aggccgagac cugcaaggug cagagcaacc gcguguucug cgacaccaug     1200

aacucccuga cgcugcccag cgagguaaac cucugcaacg ucgacaucuu caaccccaag     1260

uacgacugca agaucaugac cuccaagacc gacgugagcu ccagcgugau caccucccug     1320

ggggccaucg ucagcugcua cggcaagacg aagugcaccg ccagcaacaa gaaccggggg     1380

aucaucaaga ccuucuccaa cggcugcgac uacgugagca acaagggcgu ggacaccgug     1440

uccgucggga acacccugua cuacgugaac aagcaggagg gcaagagccu cuacgugaag     1500

ggggagccca ucaucaacuu cuacgacccg cuggucuucc ccuccgacga guucgacgcg     1560

agcaucuccc aggugaacga gaagaucaac cagagccugg ccuucauccg caaguccgac     1620

gagcugcucc acaacgugaa cgccggcaag agcacgacca acaucaugau caccaccauc     1680

aucaucguca ucaucgugau ccugcugucc cucaucgccg uggggcugcu gcucuacugc     1740

aaggcccggu gaggacuagu acuggcugca ucucccuuuc cucuguccuc cauccuucuc     1800

ccaggauggu gaagggggac cugguaccca gugaucccca ccccaggauc cuaaaucaug     1860

acuuaccugc uaauaaaaac ucauuggaaa agugagaaga ucuaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc     1980

cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau u              2031


<210>  124
<211>  2160
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  124
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu agugaaggga     1680

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa     1740

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu     1800

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua     1860

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua     1920

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     1980

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca     2100

uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc caccagaauu     2160


<210>  125
<211>  2101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  125
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu agugaaggga     1680

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa     1740

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu     1800

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua     1860

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua     1920

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     1980

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau     2100

u                                                                     2101


<210>  126
<211>  1167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  126
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu      720

aauuaagagu gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac      780

accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc      840

uuagauguuc caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc      900

cacaaaaguu cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa      960

uaaaugaaau ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa     1020

uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc ccccccccca     1140

aaggcucuuu ucagagccac cagaauu                                         1167


<210>  127
<211>  1108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  127
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu      720

aauuaagagu gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac      780

accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc      840

uuagauguuc caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc      900

cacaaaaguu cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa      960

uaaaugaaau ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa     1020

uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        1108


<210>  128
<211>  2238
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  128
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag ugaagggaac acccaaauuu aauucagccu uaagcacaau uaauuaagag     1800

ugaaacguaa uuguacaagc aguuggucac ccaccauagg gcaugaucaa caccgcaacc     1860

uuuccuuuuu cccccaguga uucugaaaaa ccccucuucc cuucagcuug cuuagauguu     1920

ccaaauuuag uaagcuuaag gcggccuaca gaagaaaaag aaaaaaaagg ccacaaaagu     1980

ucccucucac uuucaguaaa uaaaauaaaa gcagcaacag aaauaaagaa auaaaugaaa     2040

uucaaaauga aauaaauauu guguugugca gcauuaaaaa aucaauaaaa auuaaaaaug     2100

agcaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu     2220

uucagagcca ccagaauu                                                   2238


<210>  129
<211>  2179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  129
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag ugaagggaac acccaaauuu aauucagccu uaagcacaau uaauuaagag     1800

ugaaacguaa uuguacaagc aguuggucac ccaccauagg gcaugaucaa caccgcaacc     1860

uuuccuuuuu cccccaguga uucugaaaaa ccccucuucc cuucagcuug cuuagauguu     1920

ccaaauuuag uaagcuuaag gcggccuaca gaagaaaaag aaaaaaaagg ccacaaaagu     1980

ucccucucac uuucaguaaa uaaaauaaaa gcagcaacag aaauaaagaa auaaaugaaa     2040

uucaaaauga aauaaauauu guguugugca gcauuaaaaa aucaauaaaa auuaaaaaug     2100

agcaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaagaauu                                                  2179


<210>  130
<211>  2247
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  130
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggagcugc ucauccugaa ggccaacgcc      120

aucaccacca uccugacggc ggugaccuuc ugcuucgcca gcggccagaa caucaccgag      180

gaguucuacc aguccacgug cagcgccguc uccaaggggu accucagcgc ccugcggacc      240

ggcugguaca ccuccgugau caccaucgag cugagcaaca ucaaggagaa caagugcaac      300

gggaccgacg ccaaggugaa gcucaucaag caggagcugg acaaguacaa gaacgcgguc      360

acggagcugc agcuccugau gcaguccacc ccggcaacca acaaccgcgc ccggcgcgag      420

cugccccggu ucaugaacua cacccucaac aacgccaaga agacgaacgu gacccugagc      480

aagaagcgca agcggcgcuu ccugggcuuc cuccuggggg ugggcuccgc caucgcgagc      540

ggcgucgccg ugugcaaggu gcugcaccuc gagggggagg ucaacaagau caagagcgcc      600

cugcugucca ccaacaaggc cguggugagc cucuccaacg gcgucagcgu gcugaccuuc      660

aaggugcugg accucaagaa cuacaucgac aagcagcugc ugcccauccu gaacaagcag      720

agcugcucca ucagcaacau cgagacggug aucgaguucc agcagaagaa caaccggcuc      780

cuggagauca cccgcgaguu cagcgugaac gccgggguca ccacccccgu guccacguac      840

augcugacca acagcgagcu ccugucccug aucaacgaca ugccgaucac caacgaccag      900

aagaagcuca ugagcaacaa cgugcagauc guccggcagc aguccuacag caucaugugc      960

aucaucaagg aggaggugcu ggcguacgug guccagcugc cccucuacgg cgugaucgac     1020

acccccugcu ggaagcugca cacgagcccc cugugcacca ccaacaccaa ggaggggucc     1080

aacaucugcc ucacgcgcac cgaccggggc ugguacugcg acaacgccgg cagcgucucc     1140

uucuucccgc aggccgagac cugcaaggug cagagcaacc gcguguucug cgacaccaug     1200

aacucccuga cgcugcccag cgagguaaac cucugcaacg ucgacaucuu caaccccaag     1260

uacgacugca agaucaugac cuccaagacc gacgugagcu ccagcgugau caccucccug     1320

ggggccaucg ucagcugcua cggcaagacg aagugcaccg ccagcaacaa gaaccggggg     1380

aucaucaaga ccuucuccaa cggcugcgac uacgugagca acaagggcgu ggacaccgug     1440

uccgucggga acacccugua cuacgugaac aagcaggagg gcaagagccu cuacgugaag     1500

ggggagccca ucaucaacuu cuacgacccg cuggucuucc ccuccgacga guucgacgcg     1560

agcaucuccc aggugaacga gaagaucaac cagagccugg ccuucauccg caaguccgac     1620

gagcugcucc acaacgugaa cgccggcaag agcacgacca acaucaugau caccaccauc     1680

aucaucguca ucaucgugau ccugcugucc cucaucgccg uggggcugcu gcucuacugc     1740

aaggcccggu gaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu     1800

aauuaagagu gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac     1860

accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc     1920

uuagauguuc caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc     1980

cacaaaaguu cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa     2040

uaaaugaaau ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa     2100

uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc ccccccccca     2220

aaggcucuuu ucagagccac cagaauu                                         2247


<210>  131
<211>  1940
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  131
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu aguggaagca     1680

uuuuccuggc ugauuaaaag aaauuacuca gcuaugguca ucuguuccug uuagaaggcu     1740

augcagcaua uuauauacua ugcgcauguu augaaaugca uaauaaaaaa uuuuaaaaaa     1800

ucuaaaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc     1920

uuuucagagc caccagaauu                                                 1940


<210>  132
<211>  1881
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  132
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu aguggaagca     1680

uuuuccuggc ugauuaaaag aaauuacuca gcuaugguca ucuguuccug uuagaaggcu     1740

augcagcaua uuauauacua ugcgcauguu augaaaugca uaauaaaaaa uuuuaaaaaa     1800

ucuaaaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaagaau u                                               1881


<210>  133
<211>  947
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  133
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu ggaagcauuu uccuggcuga uuaaaagaaa uuacucagcu      720

auggucaucu guuccuguua gaaggcuaug cagcauauua uauacuaugc gcauguuaug      780

aaaugcauaa uaaaaaauuu uaaaaaaucu aaaagaucua aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc      900

cccccccccc ccccccccca aaggcucuuu ucagagccac cagaauu                    947


<210>  134
<211>  888
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  134
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu ggaagcauuu uccuggcuga uuaaaagaaa uuacucagcu      720

auggucaucu guuccuguua gaaggcuaug cagcauauua uauacuaugc gcauguuaug      780

aaaugcauaa uaaaaaauuu uaaaaaaucu aaaagaucua aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                   888


<210>  135
<211>  2018
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  135
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag uggaagcauu uuccuggcug auuaaaagaa auuacucagc uauggucauc     1800

uguuccuguu agaaggcuau gcagcauauu auauacuaug cgcauguuau gaaaugcaua     1860

auaaaaaauu uuaaaaaauc uaaaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc     1980

cccccccccc aaaggcucuu uucagagcca ccagaauu                             2018


<210>  136
<211>  1959
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  136
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag uggaagcauu uuccuggcug auuaaaagaa auuacucagc uauggucauc     1800

uguuccuguu agaaggcuau gcagcauauu auauacuaug cgcauguuau gaaaugcaua     1860

auaaaaaauu uuaaaaaauc uaaaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            1959


<210>  137
<211>  2027
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  137
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggagcugc ucauccugaa ggccaacgcc      120

aucaccacca uccugacggc ggugaccuuc ugcuucgcca gcggccagaa caucaccgag      180

gaguucuacc aguccacgug cagcgccguc uccaaggggu accucagcgc ccugcggacc      240

ggcugguaca ccuccgugau caccaucgag cugagcaaca ucaaggagaa caagugcaac      300

gggaccgacg ccaaggugaa gcucaucaag caggagcugg acaaguacaa gaacgcgguc      360

acggagcugc agcuccugau gcaguccacc ccggcaacca acaaccgcgc ccggcgcgag      420

cugccccggu ucaugaacua cacccucaac aacgccaaga agacgaacgu gacccugagc      480

aagaagcgca agcggcgcuu ccugggcuuc cuccuggggg ugggcuccgc caucgcgagc      540

ggcgucgccg ugugcaaggu gcugcaccuc gagggggagg ucaacaagau caagagcgcc      600

cugcugucca ccaacaaggc cguggugagc cucuccaacg gcgucagcgu gcugaccuuc      660

aaggugcugg accucaagaa cuacaucgac aagcagcugc ugcccauccu gaacaagcag      720

agcugcucca ucagcaacau cgagacggug aucgaguucc agcagaagaa caaccggcuc      780

cuggagauca cccgcgaguu cagcgugaac gccgggguca ccacccccgu guccacguac      840

augcugacca acagcgagcu ccugucccug aucaacgaca ugccgaucac caacgaccag      900

aagaagcuca ugagcaacaa cgugcagauc guccggcagc aguccuacag caucaugugc      960

aucaucaagg aggaggugcu ggcguacgug guccagcugc cccucuacgg cgugaucgac     1020

acccccugcu ggaagcugca cacgagcccc cugugcacca ccaacaccaa ggaggggucc     1080

aacaucugcc ucacgcgcac cgaccggggc ugguacugcg acaacgccgg cagcgucucc     1140

uucuucccgc aggccgagac cugcaaggug cagagcaacc gcguguucug cgacaccaug     1200

aacucccuga cgcugcccag cgagguaaac cucugcaacg ucgacaucuu caaccccaag     1260

uacgacugca agaucaugac cuccaagacc gacgugagcu ccagcgugau caccucccug     1320

ggggccaucg ucagcugcua cggcaagacg aagugcaccg ccagcaacaa gaaccggggg     1380

aucaucaaga ccuucuccaa cggcugcgac uacgugagca acaagggcgu ggacaccgug     1440

uccgucggga acacccugua cuacgugaac aagcaggagg gcaagagccu cuacgugaag     1500

ggggagccca ucaucaacuu cuacgacccg cuggucuucc ccuccgacga guucgacgcg     1560

agcaucuccc aggugaacga gaagaucaac cagagccugg ccuucauccg caaguccgac     1620

gagcugcucc acaacgugaa cgccggcaag agcacgacca acaucaugau caccaccauc     1680

aucaucguca ucaucgugau ccugcugucc cucaucgccg uggggcugcu gcucuacugc     1740

aaggcccggu gaggacuagu ggaagcauuu uccuggcuga uuaaaagaaa uuacucagcu     1800

auggucaucu guuccuguua gaaggcuaug cagcauauua uauacuaugc gcauguuaug     1860

aaaugcauaa uaaaaaauuu uaaaaaaucu aaaagaucua aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc     1980

cccccccccc ccccccccca aaggcucuuu ucagagccac cagaauu                   2027


<210>  138
<211>  1877
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  138
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu aguguccacc     1680

ugucccuccu gggcugcugg auugucucgu uuuccugcca aauaaacagg aucagcgcuu     1740

uacagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu     1860

ucagagccac cagaauu                                                    1877


<210>  139
<211>  1818
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  139
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggugcccc aggcccuccu guucgucccg      120

cugcuggugu ucccccucug cuucggcaag uuccccaucu acaccauccc cgacaagcug      180

gggccgugga gccccaucga cauccaccac cuguccugcc ccaacaaccu cguggucgag      240

gacgagggcu gcaccaaccu gagcggguuc uccuacaugg agcugaaggu gggcuacauc      300

agcgccauca agaugaacgg guucacgugc accggcgugg ucaccgaggc ggagaccuac      360

acgaacuucg ugggcuacgu gaccaccacc uucaagcgga agcacuuccg ccccacgccg      420

gacgccugcc gggccgccua caacuggaag auggccgggg acccccgcua cgaggagucc      480

cuccacaacc ccuaccccga cuaccacugg cugcggaccg ucaagaccac caaggagagc      540

cuggugauca ucuccccgag cguggcggac cucgaccccu acgaccgcuc ccugcacagc      600

cgggucuucc ccggcgggaa cugcuccggc guggccguga gcuccacgua cugcagcacc      660

aaccacgacu acaccaucug gaugcccgag aacccgcgcc uggggauguc cugcgacauc      720

uucaccaaca gccggggcaa gcgcgccucc aagggcagcg agacgugcgg guucgucgac      780

gagcggggcc ucuacaaguc ccugaagggg gccugcaagc ugaagcucug cggcgugcug      840

ggccugcgcc ucauggacgg gaccugggug gcgaugcaga ccagcaacga gaccaagugg      900

ugcccccccg gccagcuggu caaccugcac gacuuccgga gcgacgagau cgagcaccuc      960

gugguggagg agcuggucaa gaagcgcgag gagugccugg acgcccucga guccaucaug     1020

acgaccaaga gcguguccuu ccggcgccug agccaccucc ggaagcuggu gcccggguuc     1080

ggcaaggccu acaccaucuu caacaagacc cugauggagg ccgacgccca cuacaagucc     1140

guccgcacgu ggaacgagau caucccgagc aaggggugcc ugcggguggg cggccgcugc     1200

cacccccacg ucaacggggu guucuucaac ggcaucaucc ucgggcccga cggcaacgug     1260

cugauccccg agaugcaguc cagccugcuc cagcagcaca uggagcugcu ggucuccagc     1320

gugaucccgc ucaugcaccc ccuggcggac cccuccaccg uguucaagaa cggggacgag     1380

gccgaggacu ucgucgaggu gcaccucccc gacgugcacg agcggaucag cggcgucgac     1440

cugggccugc cgaacugggg gaaguacgug cugcucuccg ccggcgcccu gaccgcccug     1500

augcugauca ucuuccucau gaccugcugg cgccggguga accggagcga gcccacgcag     1560

cacaaccugc gcgggaccgg ccgggagguc uccgugaccc cgcagagcgg gaagaucauc     1620

uccagcuggg aguccuacaa gagcggcggc gagaccgggc ugugaggacu aguguccacc     1680

ugucccuccu gggcugcugg auugucucgu uuuccugcca aauaaacagg aucagcgcuu     1740

uacagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaagaauu                                                   1818


<210>  140
<211>  884
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  140
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu      720

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc      840

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                       884


<210>  141
<211>  825
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  141
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc augggcgugc acgagugccc cgccuggcug      120

uggcuccugc ugagccuccu gucccugccg cucgggcugc ccguccuggg cgcccccccg      180

cggcucaucu gcgacagccg cgugcuggag cgguaccugc ucgaggcgaa ggaggccgag      240

aacaucacca ccgggugcgc cgagcacugc ucccugaacg agaacaucac ggugccggac      300

accaagguca acuucuacgc cuggaagcgc auggaggugg gccagcaggc cguggagguc      360

uggcaggggc uggcgcuccu gagcgaggcc gugcugcggg gccaggcccu ccuggugaac      420

uccagccagc ccugggagcc ccugcagcuc cacgucgaca aggccguguc cggccugcgc      480

agccugacca cccuccugcg ggcgcugggg gcccagaagg aggccaucuc ccccccggac      540

gccgccagcg cggccccgcu ccgcacgauc accgccgaca ccuuccggaa gcuguuccgc      600

guguacucca acuuccugcg gggcaagcuc aagcuguaca ccggggaggc cugccgcacg      660

ggcgaccggu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu      720

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                      825


<210>  142
<211>  1955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  142
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag uguccaccug ucccuccugg gcugcuggau ugucucguuu uccugccaaa     1800

uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc     1920

cccccccaaa ggcucuuuuc agagccacca gaauu                                1955


<210>  143
<211>  1896
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  143
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggaggacg ccaagaacau caagaagggc      120

ccggcgcccu ucuacccgcu ggaggacggg accgccggcg agcagcucca caaggccaug      180

aagcgguacg cccuggugcc gggcacgauc gccuucaccg acgcccacau cgaggucgac      240

aucaccuacg cggaguacuu cgagaugagc gugcgccugg ccgaggccau gaagcgguac      300

ggccugaaca ccaaccaccg gaucguggug ugcucggaga acagccugca guucuucaug      360

ccggugcugg gcgcccucuu caucggcgug gccgucgccc cggcgaacga caucuacaac      420

gagcgggagc ugcugaacag cauggggauc agccagccga ccgugguguu cgugagcaag      480

aagggccugc agaagauccu gaacgugcag aagaagcugc ccaucaucca gaagaucauc      540

aucauggaca gcaagaccga cuaccagggc uuccagucga uguacacguu cgugaccagc      600

caccucccgc cgggcuucaa cgaguacgac uucgucccgg agagcuucga ccgggacaag      660

accaucgccc ugaucaugaa cagcagcggc agcaccggcc ugccgaaggg gguggcccug      720

ccgcaccgga ccgccugcgu gcgcuucucg cacgcccggg accccaucuu cggcaaccag      780

aucaucccgg acaccgccau ccugagcgug gugccguucc accacggcuu cggcauguuc      840

acgacccugg gcuaccucau cugcggcuuc cggguggucc ugauguaccg guucgaggag      900

gagcuguucc ugcggagccu gcaggacuac aagauccaga gcgcgcugcu cgugccgacc      960

cuguucagcu ucuucgccaa gagcacccug aucgacaagu acgaccuguc gaaccugcac     1020

gagaucgcca gcgggggcgc cccgcugagc aaggaggugg gcgaggccgu ggccaagcgg     1080

uuccaccucc cgggcauccg ccagggcuac ggccugaccg agaccacgag cgcgauccug     1140

aucacccccg agggggacga caagccgggc gccgugggca aggugguccc guucuucgag     1200

gccaaggugg uggaccugga caccggcaag acccugggcg ugaaccagcg gggcgagcug     1260

ugcgugcggg ggccgaugau caugagcggc uacgugaaca acccggaggc caccaacgcc     1320

cucaucgaca aggacggcug gcugcacagc ggcgacaucg ccuacuggga cgaggacgag     1380

cacuucuuca ucgucgaccg gcugaagucg cugaucaagu acaagggcua ccagguggcg     1440

ccggccgagc uggagagcau ccugcuccag caccccaaca ucuucgacgc cggcguggcc     1500

gggcugccgg acgacgacgc cggcgagcug ccggccgcgg ugguggugcu ggagcacggc     1560

aagaccauga cggagaagga gaucgucgac uacguggcca gccaggugac caccgccaag     1620

aagcugcggg gcggcguggu guucguggac gaggucccga agggccugac cgggaagcuc     1680

gacgcccgga agauccgcga gauccugauc aaggccaaga agggcggcaa gaucgccgug     1740

ugaggacuag uguccaccug ucccuccugg gcugcuggau ugucucguuu uccugccaaa     1800

uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               1896


<210>  144
<211>  1964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  144
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggagcugc ucauccugaa ggccaacgcc      120

aucaccacca uccugacggc ggugaccuuc ugcuucgcca gcggccagaa caucaccgag      180

gaguucuacc aguccacgug cagcgccguc uccaaggggu accucagcgc ccugcggacc      240

ggcugguaca ccuccgugau caccaucgag cugagcaaca ucaaggagaa caagugcaac      300

gggaccgacg ccaaggugaa gcucaucaag caggagcugg acaaguacaa gaacgcgguc      360

acggagcugc agcuccugau gcaguccacc ccggcaacca acaaccgcgc ccggcgcgag      420

cugccccggu ucaugaacua cacccucaac aacgccaaga agacgaacgu gacccugagc      480

aagaagcgca agcggcgcuu ccugggcuuc cuccuggggg ugggcuccgc caucgcgagc      540

ggcgucgccg ugugcaaggu gcugcaccuc gagggggagg ucaacaagau caagagcgcc      600

cugcugucca ccaacaaggc cguggugagc cucuccaacg gcgucagcgu gcugaccuuc      660

aaggugcugg accucaagaa cuacaucgac aagcagcugc ugcccauccu gaacaagcag      720

agcugcucca ucagcaacau cgagacggug aucgaguucc agcagaagaa caaccggcuc      780

cuggagauca cccgcgaguu cagcgugaac gccgggguca ccacccccgu guccacguac      840

augcugacca acagcgagcu ccugucccug aucaacgaca ugccgaucac caacgaccag      900

aagaagcuca ugagcaacaa cgugcagauc guccggcagc aguccuacag caucaugugc      960

aucaucaagg aggaggugcu ggcguacgug guccagcugc cccucuacgg cgugaucgac     1020

acccccugcu ggaagcugca cacgagcccc cugugcacca ccaacaccaa ggaggggucc     1080

aacaucugcc ucacgcgcac cgaccggggc ugguacugcg acaacgccgg cagcgucucc     1140

uucuucccgc aggccgagac cugcaaggug cagagcaacc gcguguucug cgacaccaug     1200

aacucccuga cgcugcccag cgagguaaac cucugcaacg ucgacaucuu caaccccaag     1260

uacgacugca agaucaugac cuccaagacc gacgugagcu ccagcgugau caccucccug     1320

ggggccaucg ucagcugcua cggcaagacg aagugcaccg ccagcaacaa gaaccggggg     1380

aucaucaaga ccuucuccaa cggcugcgac uacgugagca acaagggcgu ggacaccgug     1440

uccgucggga acacccugua cuacgugaac aagcaggagg gcaagagccu cuacgugaag     1500

ggggagccca ucaucaacuu cuacgacccg cuggucuucc ccuccgacga guucgacgcg     1560

agcaucuccc aggugaacga gaagaucaac cagagccugg ccuucauccg caaguccgac     1620

gagcugcucc acaacgugaa cgccggcaag agcacgacca acaucaugau caccaccauc     1680

aucaucguca ucaucgugau ccugcugucc cucaucgccg uggggcugcu gcucuacugc     1740

aaggcccggu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu     1800

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc     1920

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                      1964


<210>  145
<211>  1843
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  145
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua gucccuguuc ccagagccca cuuuuuuuuc     1680

uuuuuuugaa auaaaauagc cugucuuuca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1800

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1843


<210>  146
<211>  1784
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  146
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua gucccuguuc ccagagccca cuuuuuuuuc     1680

uuuuuuugaa auaaaauagc cugucuuuca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      1784


<210>  147
<211>  850
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  147
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuaguc      660

ccuguuccca gagcccacuu uuuuuucuuu uuuugaaaua aaauagccug ucuuucagau      720

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc      840

caccagaauu                                                             850


<210>  148
<211>  791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  148
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuaguc      660

ccuguuccca gagcccacuu uuuuuucuuu uuuugaaaua aaauagccug ucuuucagau      720

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaagaau u                                                           791


<210>  149
<211>  1921
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  149
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu cccuguuccc     1740

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1860

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1920

u                                                                     1921


<210>  150
<211>  1862
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  150
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu cccuguuccc     1740

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     1860

uu                                                                    1862


<210>  151
<211>  1930
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  151
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggagcugcu cauccugaag gccaacgcca ucaccaccau ccugacggcg      120

gugaccuucu gcuucgccag cggccagaac aucaccgagg aguucuacca guccacgugc      180

agcgccgucu ccaaggggua ccucagcgcc cugcggaccg gcugguacac cuccgugauc      240

accaucgagc ugagcaacau caaggagaac aagugcaacg ggaccgacgc caaggugaag      300

cucaucaagc aggagcugga caaguacaag aacgcgguca cggagcugca gcuccugaug      360

caguccaccc cggcaaccaa caaccgcgcc cggcgcgagc ugccccgguu caugaacuac      420

acccucaaca acgccaagaa gacgaacgug acccugagca agaagcgcaa gcggcgcuuc      480

cugggcuucc uccugggggu gggcuccgcc aucgcgagcg gcgucgccgu gugcaaggug      540

cugcaccucg agggggaggu caacaagauc aagagcgccc ugcuguccac caacaaggcc      600

guggugagcc ucuccaacgg cgucagcgug cugaccuuca aggugcugga ccucaagaac      660

uacaucgaca agcagcugcu gcccauccug aacaagcaga gcugcuccau cagcaacauc      720

gagacgguga ucgaguucca gcagaagaac aaccggcucc uggagaucac ccgcgaguuc      780

agcgugaacg ccggggucac cacccccgug uccacguaca ugcugaccaa cagcgagcuc      840

cugucccuga ucaacgacau gccgaucacc aacgaccaga agaagcucau gagcaacaac      900

gugcagaucg uccggcagca guccuacagc aucaugugca ucaucaagga ggaggugcug      960

gcguacgugg uccagcugcc ccucuacggc gugaucgaca cccccugcug gaagcugcac     1020

acgagccccc ugugcaccac caacaccaag gaggggucca acaucugccu cacgcgcacc     1080

gaccggggcu gguacugcga caacgccggc agcgucuccu ucuucccgca ggccgagacc     1140

ugcaaggugc agagcaaccg cguguucugc gacaccauga acucccugac gcugcccagc     1200

gagguaaacc ucugcaacgu cgacaucuuc aaccccaagu acgacugcaa gaucaugacc     1260

uccaagaccg acgugagcuc cagcgugauc accucccugg gggccaucgu cagcugcuac     1320

ggcaagacga agugcaccgc cagcaacaag aaccggggga ucaucaagac cuucuccaac     1380

ggcugcgacu acgugagcaa caagggcgug gacaccgugu ccgucgggaa cacccuguac     1440

uacgugaaca agcaggaggg caagagccuc uacgugaagg gggagcccau caucaacuuc     1500

uacgacccgc uggucuuccc cuccgacgag uucgacgcga gcaucuccca ggugaacgag     1560

aagaucaacc agagccuggc cuucauccgc aaguccgacg agcugcucca caacgugaac     1620

gccggcaaga gcacgaccaa caucaugauc accaccauca ucaucgucau caucgugauc     1680

cugcuguccc ucaucgccgu ggggcugcug cucuacugca aggcccggug aggacuaguc     1740

ccuguuccca gagcccacuu uuuuuucuuu uuuugaaaua aaauagccug ucuuucagau     1800

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1920

caccagaauu                                                            1930


<210>  152
<211>  1907
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  152
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua guaauaagga aacuguauga augucugugg     1680

gcaggaagug aagagauccu ucuguaaagg uuuuuggaau uaugucugcu gaauaauaaa     1740

cuuuuuugaa auaauaaauc ugguagaaaa augagaucua aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc     1860

cccccccccc ccccccccca aaggcucuuu ucagagccac cagaauu                   1907


<210>  153
<211>  1848
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  153
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua guaauaagga aacuguauga augucugugg     1680

gcaggaagug aagagauccu ucuguaaagg uuuuuggaau uaugucugcu gaauaauaaa     1740

cuuuuuugaa auaauaaauc ugguagaaaa augagaucua aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  1848


<210>  154
<211>  914
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  154
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagua      660

auaaggaaac uguaugaaug ucugugggca ggaagugaag agauccuucu guaaagguuu      720

uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua auaaaucugg uagaaaaaug      780

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca      900

gagccaccag aauu                                                        914


<210>  155
<211>  855
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  155
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagua      660

auaaggaaac uguaugaaug ucugugggca ggaagugaag agauccuucu guaaagguuu      720

uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua auaaaucugg uagaaaaaug      780

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa gaauu                                                       855


<210>  156
<211>  1985
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  156
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu aauaaggaaa     1740

cuguaugaau gucugugggc aggaagugaa gagauccuuc uguaaagguu uuuggaauua     1800

ugucugcuga auaauaaacu uuuuugaaau aauaaaucug guagaaaaau gagaucuaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

augcaucccc cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca     1980

gaauu                                                                 1985


<210>  157
<211>  1926
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  157
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu aauaaggaaa     1740

cuguaugaau gucugugggc aggaagugaa gagauccuuc uguaaagguu uuuggaauua     1800

ugucugcuga auaauaaacu uuuuugaaau aauaaaucug guagaaaaau gagaucuaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

agaauu                                                                1926


<210>  158
<211>  1994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  158
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggagcugcu cauccugaag gccaacgcca ucaccaccau ccugacggcg      120

gugaccuucu gcuucgccag cggccagaac aucaccgagg aguucuacca guccacgugc      180

agcgccgucu ccaaggggua ccucagcgcc cugcggaccg gcugguacac cuccgugauc      240

accaucgagc ugagcaacau caaggagaac aagugcaacg ggaccgacgc caaggugaag      300

cucaucaagc aggagcugga caaguacaag aacgcgguca cggagcugca gcuccugaug      360

caguccaccc cggcaaccaa caaccgcgcc cggcgcgagc ugccccgguu caugaacuac      420

acccucaaca acgccaagaa gacgaacgug acccugagca agaagcgcaa gcggcgcuuc      480

cugggcuucc uccugggggu gggcuccgcc aucgcgagcg gcgucgccgu gugcaaggug      540

cugcaccucg agggggaggu caacaagauc aagagcgccc ugcuguccac caacaaggcc      600

guggugagcc ucuccaacgg cgucagcgug cugaccuuca aggugcugga ccucaagaac      660

uacaucgaca agcagcugcu gcccauccug aacaagcaga gcugcuccau cagcaacauc      720

gagacgguga ucgaguucca gcagaagaac aaccggcucc uggagaucac ccgcgaguuc      780

agcgugaacg ccggggucac cacccccgug uccacguaca ugcugaccaa cagcgagcuc      840

cugucccuga ucaacgacau gccgaucacc aacgaccaga agaagcucau gagcaacaac      900

gugcagaucg uccggcagca guccuacagc aucaugugca ucaucaagga ggaggugcug      960

gcguacgugg uccagcugcc ccucuacggc gugaucgaca cccccugcug gaagcugcac     1020

acgagccccc ugugcaccac caacaccaag gaggggucca acaucugccu cacgcgcacc     1080

gaccggggcu gguacugcga caacgccggc agcgucuccu ucuucccgca ggccgagacc     1140

ugcaaggugc agagcaaccg cguguucugc gacaccauga acucccugac gcugcccagc     1200

gagguaaacc ucugcaacgu cgacaucuuc aaccccaagu acgacugcaa gaucaugacc     1260

uccaagaccg acgugagcuc cagcgugauc accucccugg gggccaucgu cagcugcuac     1320

ggcaagacga agugcaccgc cagcaacaag aaccggggga ucaucaagac cuucuccaac     1380

ggcugcgacu acgugagcaa caagggcgug gacaccgugu ccgucgggaa cacccuguac     1440

uacgugaaca agcaggaggg caagagccuc uacgugaagg gggagcccau caucaacuuc     1500

uacgacccgc uggucuuccc cuccgacgag uucgacgcga gcaucuccca ggugaacgag     1560

aagaucaacc agagccuggc cuucauccgc aaguccgacg agcugcucca caacgugaac     1620

gccggcaaga gcacgaccaa caucaugauc accaccauca ucaucgucau caucgugauc     1680

cugcuguccc ucaucgccgu ggggcugcug cucuacugca aggcccggug aggacuagua     1740

auaaggaaac uguaugaaug ucugugggca ggaagugaag agauccuucu guaaagguuu     1800

uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua auaaaucugg uagaaaaaug     1860

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca     1980

gagccaccag aauu                                                       1994


<210>  159
<211>  1923
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  159
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua guacuggcug caucucccuu uccucugucc     1680

uccauccuuc ucccaggaug gugaaggggg accugguacc cagugauccc caccccagga     1740

uccuaaauca ugacuuaccu gcuaauaaaa acucauugga aaagugagaa gaucuaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau     1860

gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag agccaccaga     1920

auu                                                                   1923


<210>  160
<211>  1864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  160
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua guacuggcug caucucccuu uccucugucc     1680

uccauccuuc ucccaggaug gugaaggggg accugguacc cagugauccc caccccagga     1740

uccuaaauca ugacuuaccu gcuaauaaaa acucauugga aaagugagaa gaucuaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag     1860

aauu                                                                  1864


<210>  161
<211>  930
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  161
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagua      660

cuggcugcau cucccuuucc ucuguccucc auccuucucc caggauggug aagggggacc      720

ugguacccag ugauccccac cccaggaucc uaaaucauga cuuaccugcu aauaaaaacu      780

cauuggaaaa gugagaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc cccccccccc      900

ccaaaggcuc uuuucagagc caccagaauu                                       930


<210>  162
<211>  871
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  162
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagua      660

cuggcugcau cucccuuucc ucuguccucc auccuucucc caggauggug aagggggacc      720

ugguacccag ugauccccac cccaggaucc uaaaucauga cuuaccugcu aauaaaaacu      780

cauuggaaaa gugagaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                                     871


<210>  163
<211>  2001
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  163
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu acuggcugca     1740

ucucccuuuc cucuguccuc cauccuucuc ccaggauggu gaagggggac cugguaccca     1800

gugaucccca ccccaggauc cuaaaucaug acuuaccugc uaauaaaaac ucauuggaaa     1860

agugagaaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu     1980

cuuuucagag ccaccagaau u                                               2001


<210>  164
<211>  1942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  164
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu acuggcugca     1740

ucucccuuuc cucuguccuc cauccuucuc ccaggauggu gaagggggac cugguaccca     1800

gugaucccca ccccaggauc cuaaaucaug acuuaccugc uaauaaaaac ucauuggaaa     1860

agugagaaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaagaa uu                                              1942


<210>  165
<211>  2010
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  165
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggagcugcu cauccugaag gccaacgcca ucaccaccau ccugacggcg      120

gugaccuucu gcuucgccag cggccagaac aucaccgagg aguucuacca guccacgugc      180

agcgccgucu ccaaggggua ccucagcgcc cugcggaccg gcugguacac cuccgugauc      240

accaucgagc ugagcaacau caaggagaac aagugcaacg ggaccgacgc caaggugaag      300

cucaucaagc aggagcugga caaguacaag aacgcgguca cggagcugca gcuccugaug      360

caguccaccc cggcaaccaa caaccgcgcc cggcgcgagc ugccccgguu caugaacuac      420

acccucaaca acgccaagaa gacgaacgug acccugagca agaagcgcaa gcggcgcuuc      480

cugggcuucc uccugggggu gggcuccgcc aucgcgagcg gcgucgccgu gugcaaggug      540

cugcaccucg agggggaggu caacaagauc aagagcgccc ugcuguccac caacaaggcc      600

guggugagcc ucuccaacgg cgucagcgug cugaccuuca aggugcugga ccucaagaac      660

uacaucgaca agcagcugcu gcccauccug aacaagcaga gcugcuccau cagcaacauc      720

gagacgguga ucgaguucca gcagaagaac aaccggcucc uggagaucac ccgcgaguuc      780

agcgugaacg ccggggucac cacccccgug uccacguaca ugcugaccaa cagcgagcuc      840

cugucccuga ucaacgacau gccgaucacc aacgaccaga agaagcucau gagcaacaac      900

gugcagaucg uccggcagca guccuacagc aucaugugca ucaucaagga ggaggugcug      960

gcguacgugg uccagcugcc ccucuacggc gugaucgaca cccccugcug gaagcugcac     1020

acgagccccc ugugcaccac caacaccaag gaggggucca acaucugccu cacgcgcacc     1080

gaccggggcu gguacugcga caacgccggc agcgucuccu ucuucccgca ggccgagacc     1140

ugcaaggugc agagcaaccg cguguucugc gacaccauga acucccugac gcugcccagc     1200

gagguaaacc ucugcaacgu cgacaucuuc aaccccaagu acgacugcaa gaucaugacc     1260

uccaagaccg acgugagcuc cagcgugauc accucccugg gggccaucgu cagcugcuac     1320

ggcaagacga agugcaccgc cagcaacaag aaccggggga ucaucaagac cuucuccaac     1380

ggcugcgacu acgugagcaa caagggcgug gacaccgugu ccgucgggaa cacccuguac     1440

uacgugaaca agcaggaggg caagagccuc uacgugaagg gggagcccau caucaacuuc     1500

uacgacccgc uggucuuccc cuccgacgag uucgacgcga gcaucuccca ggugaacgag     1560

aagaucaacc agagccuggc cuucauccgc aaguccgacg agcugcucca caacgugaac     1620

gccggcaaga gcacgaccaa caucaugauc accaccauca ucaucgucau caucgugauc     1680

cugcuguccc ucaucgccgu ggggcugcug cucuacugca aggcccggug aggacuagua     1740

cuggcugcau cucccuuucc ucuguccucc auccuucucc caggauggug aagggggacc     1800

ugguacccag ugauccccac cccaggaucc uaaaucauga cuuaccugcu aauaaaaacu     1860

cauuggaaaa gugagaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc cccccccccc     1980

ccaaaggcuc uuuucagagc caccagaauu                                      2010


<210>  166
<211>  2139
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  166
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua gugaagggaa cacccaaauu uaauucagcc     1680

uuaagcacaa uuaauuaaga gugaaacgua auuguacaag caguugguca cccaccauag     1740

ggcaugauca acaccgcaac cuuuccuuuu ucccccagug auucugaaaa accccucuuc     1800

ccuucagcuu gcuuagaugu uccaaauuua guaagcuuaa ggcggccuac agaagaaaaa     1860

gaaaaaaaag gccacaaaag uucccucuca cuuucaguaa auaaaauaaa agcagcaaca     1920

gaaauaaaga aauaaaugaa auucaaaaug aaauaaauau uguguugugc agcauuaaaa     1980

aaucaauaaa aauuaaaaau gagcaagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc     2100

cccccccccc caaaggcucu uuucagagcc accagaauu                            2139


<210>  167
<211>  2080
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  167
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua gugaagggaa cacccaaauu uaauucagcc     1680

uuaagcacaa uuaauuaaga gugaaacgua auuguacaag caguugguca cccaccauag     1740

ggcaugauca acaccgcaac cuuuccuuuu ucccccagug auucugaaaa accccucuuc     1800

ccuucagcuu gcuuagaugu uccaaauuua guaagcuuaa ggcggccuac agaagaaaaa     1860

gaaaaaaaag gccacaaaag uucccucuca cuuucaguaa auaaaauaaa agcagcaaca     1920

gaaauaaaga aauaaaugaa auucaaaaug aaauaaauau uguguugugc agcauuaaaa     1980

aaucaauaaa aauuaaaaau gagcaagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                           2080


<210>  168
<211>  1146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  168
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagug      660

aagggaacac ccaaauuuaa uucagccuua agcacaauua auuaagagug aaacguaauu      720

guacaagcag uuggucaccc accauagggc augaucaaca ccgcaaccuu uccuuuuucc      780

cccagugauu cugaaaaacc ccucuucccu ucagcuugcu uagauguucc aaauuuagua      840

agcuuaaggc ggccuacaga agaaaaagaa aaaaaaggcc acaaaaguuc ccucucacuu      900

ucaguaaaua aaauaaaagc agcaacagaa auaaagaaau aaaugaaauu caaaaugaaa      960

uaaauauugu guugugcagc auuaaaaaau caauaaaaau uaaaaaugag caagaucuaa     1020

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu cagagccacc     1140

agaauu                                                                1146


<210>  169
<211>  1087
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  169
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagug      660

aagggaacac ccaaauuuaa uucagccuua agcacaauua auuaagagug aaacguaauu      720

guacaagcag uuggucaccc accauagggc augaucaaca ccgcaaccuu uccuuuuucc      780

cccagugauu cugaaaaacc ccucuucccu ucagcuugcu uagauguucc aaauuuagua      840

agcuuaaggc ggccuacaga agaaaaagaa aaaaaaggcc acaaaaguuc ccucucacuu      900

ucaguaaaua aaauaaaagc agcaacagaa auaaagaaau aaaugaaauu caaaaugaaa      960

uaaauauugu guugugcagc auuaaaaaau caauaaaaau uaaaaaugag caagaucuaa     1020

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aagaauu                                                               1087


<210>  170
<211>  2217
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  170
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu gaagggaaca     1740

cccaaauuua auucagccuu aagcacaauu aauuaagagu gaaacguaau uguacaagca     1800

guuggucacc caccauaggg caugaucaac accgcaaccu uuccuuuuuc ccccagugau     1860

ucugaaaaac cccucuuccc uucagcuugc uuagauguuc caaauuuagu aagcuuaagg     1920

cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu cccucucacu uucaguaaau     1980

aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau ucaaaaugaa auaaauauug     2040

uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga gcaagaucua aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc     2160

cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac cagaauu        2217


<210>  171
<211>  2158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  171
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu gaagggaaca     1740

cccaaauuua auucagccuu aagcacaauu aauuaagagu gaaacguaau uguacaagca     1800

guuggucacc caccauaggg caugaucaac accgcaaccu uuccuuuuuc ccccagugau     1860

ucugaaaaac cccucuuccc uucagcuugc uuagauguuc caaauuuagu aagcuuaagg     1920

cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu cccucucacu uucaguaaau     1980

aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau ucaaaaugaa auaaauauug     2040

uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga gcaagaucua aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu       2158


<210>  172
<211>  2226
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  172
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggagcugcu cauccugaag gccaacgcca ucaccaccau ccugacggcg      120

gugaccuucu gcuucgccag cggccagaac aucaccgagg aguucuacca guccacgugc      180

agcgccgucu ccaaggggua ccucagcgcc cugcggaccg gcugguacac cuccgugauc      240

accaucgagc ugagcaacau caaggagaac aagugcaacg ggaccgacgc caaggugaag      300

cucaucaagc aggagcugga caaguacaag aacgcgguca cggagcugca gcuccugaug      360

caguccaccc cggcaaccaa caaccgcgcc cggcgcgagc ugccccgguu caugaacuac      420

acccucaaca acgccaagaa gacgaacgug acccugagca agaagcgcaa gcggcgcuuc      480

cugggcuucc uccugggggu gggcuccgcc aucgcgagcg gcgucgccgu gugcaaggug      540

cugcaccucg agggggaggu caacaagauc aagagcgccc ugcuguccac caacaaggcc      600

guggugagcc ucuccaacgg cgucagcgug cugaccuuca aggugcugga ccucaagaac      660

uacaucgaca agcagcugcu gcccauccug aacaagcaga gcugcuccau cagcaacauc      720

gagacgguga ucgaguucca gcagaagaac aaccggcucc uggagaucac ccgcgaguuc      780

agcgugaacg ccggggucac cacccccgug uccacguaca ugcugaccaa cagcgagcuc      840

cugucccuga ucaacgacau gccgaucacc aacgaccaga agaagcucau gagcaacaac      900

gugcagaucg uccggcagca guccuacagc aucaugugca ucaucaagga ggaggugcug      960

gcguacgugg uccagcugcc ccucuacggc gugaucgaca cccccugcug gaagcugcac     1020

acgagccccc ugugcaccac caacaccaag gaggggucca acaucugccu cacgcgcacc     1080

gaccggggcu gguacugcga caacgccggc agcgucuccu ucuucccgca ggccgagacc     1140

ugcaaggugc agagcaaccg cguguucugc gacaccauga acucccugac gcugcccagc     1200

gagguaaacc ucugcaacgu cgacaucuuc aaccccaagu acgacugcaa gaucaugacc     1260

uccaagaccg acgugagcuc cagcgugauc accucccugg gggccaucgu cagcugcuac     1320

ggcaagacga agugcaccgc cagcaacaag aaccggggga ucaucaagac cuucuccaac     1380

ggcugcgacu acgugagcaa caagggcgug gacaccgugu ccgucgggaa cacccuguac     1440

uacgugaaca agcaggaggg caagagccuc uacgugaagg gggagcccau caucaacuuc     1500

uacgacccgc uggucuuccc cuccgacgag uucgacgcga gcaucuccca ggugaacgag     1560

aagaucaacc agagccuggc cuucauccgc aaguccgacg agcugcucca caacgugaac     1620

gccggcaaga gcacgaccaa caucaugauc accaccauca ucaucgucau caucgugauc     1680

cugcuguccc ucaucgccgu ggggcugcug cucuacugca aggcccggug aggacuagug     1740

aagggaacac ccaaauuuaa uucagccuua agcacaauua auuaagagug aaacguaauu     1800

guacaagcag uuggucaccc accauagggc augaucaaca ccgcaaccuu uccuuuuucc     1860

cccagugauu cugaaaaacc ccucuucccu ucagcuugcu uagauguucc aaauuuagua     1920

agcuuaaggc ggccuacaga agaaaaagaa aaaaaaggcc acaaaaguuc ccucucacuu     1980

ucaguaaaua aaauaaaagc agcaacagaa auaaagaaau aaaugaaauu caaaaugaaa     2040

uaaauauugu guugugcagc auuaaaaaau caauaaaaau uaaaaaugag caagaucuaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu cagagccacc     2220

agaauu                                                                2226


<210>  173
<211>  1919
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  173
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua guggaagcau uuuccuggcu gauuaaaaga     1680

aauuacucag cuauggucau cuguuccugu uagaaggcua ugcagcauau uauauacuau     1740

gcgcauguua ugaaaugcau aauaaaaaau uuuaaaaaau cuaaaagauc uaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau     1860

cccccccccc cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu      1919


<210>  174
<211>  1860
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  174
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua guggaagcau uuuccuggcu gauuaaaaga     1680

aauuacucag cuauggucau cuguuccugu uagaaggcua ugcagcauau uauauacuau     1740

gcgcauguua ugaaaugcau aauaaaaaau uuuaaaaaau cuaaaagauc uaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     1860


<210>  175
<211>  926
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  175
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagug      660

gaagcauuuu ccuggcugau uaaaagaaau uacucagcua uggucaucug uuccuguuag      720

aaggcuaugc agcauauuau auacuaugcg cauguuauga aaugcauaau aaaaaauuuu      780

aaaaaaucua aaagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa      900

aggcucuuuu cagagccacc agaauu                                           926


<210>  176
<211>  867
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  176
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagug      660

gaagcauuuu ccuggcugau uaaaagaaau uacucagcua uggucaucug uuccuguuag      720

aaggcuaugc agcauauuau auacuaugcg cauguuauga aaugcauaau aaaaaauuuu      780

aaaaaaucua aaagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aagaauu                                          867


<210>  177
<211>  1997
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  177
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu ggaagcauuu     1740

uccuggcuga uuaaaagaaa uuacucagcu auggucaucu guuccuguua gaaggcuaug     1800

cagcauauua uauacuaugc gcauguuaug aaaugcauaa uaaaaaauuu uaaaaaaucu     1860

aaaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu     1980

ucagagccac cagaauu                                                    1997


<210>  178
<211>  1938
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  178
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu ggaagcauuu     1740

uccuggcuga uuaaaagaaa uuacucagcu auggucaucu guuccuguua gaaggcuaug     1800

cagcauauua uauacuaugc gcauguuaug aaaugcauaa uaaaaaauuu uaaaaaaucu     1860

aaaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaagaauu                                                   1938


<210>  179
<211>  2006
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  179
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggagcugcu cauccugaag gccaacgcca ucaccaccau ccugacggcg      120

gugaccuucu gcuucgccag cggccagaac aucaccgagg aguucuacca guccacgugc      180

agcgccgucu ccaaggggua ccucagcgcc cugcggaccg gcugguacac cuccgugauc      240

accaucgagc ugagcaacau caaggagaac aagugcaacg ggaccgacgc caaggugaag      300

cucaucaagc aggagcugga caaguacaag aacgcgguca cggagcugca gcuccugaug      360

caguccaccc cggcaaccaa caaccgcgcc cggcgcgagc ugccccgguu caugaacuac      420

acccucaaca acgccaagaa gacgaacgug acccugagca agaagcgcaa gcggcgcuuc      480

cugggcuucc uccugggggu gggcuccgcc aucgcgagcg gcgucgccgu gugcaaggug      540

cugcaccucg agggggaggu caacaagauc aagagcgccc ugcuguccac caacaaggcc      600

guggugagcc ucuccaacgg cgucagcgug cugaccuuca aggugcugga ccucaagaac      660

uacaucgaca agcagcugcu gcccauccug aacaagcaga gcugcuccau cagcaacauc      720

gagacgguga ucgaguucca gcagaagaac aaccggcucc uggagaucac ccgcgaguuc      780

agcgugaacg ccggggucac cacccccgug uccacguaca ugcugaccaa cagcgagcuc      840

cugucccuga ucaacgacau gccgaucacc aacgaccaga agaagcucau gagcaacaac      900

gugcagaucg uccggcagca guccuacagc aucaugugca ucaucaagga ggaggugcug      960

gcguacgugg uccagcugcc ccucuacggc gugaucgaca cccccugcug gaagcugcac     1020

acgagccccc ugugcaccac caacaccaag gaggggucca acaucugccu cacgcgcacc     1080

gaccggggcu gguacugcga caacgccggc agcgucuccu ucuucccgca ggccgagacc     1140

ugcaaggugc agagcaaccg cguguucugc gacaccauga acucccugac gcugcccagc     1200

gagguaaacc ucugcaacgu cgacaucuuc aaccccaagu acgacugcaa gaucaugacc     1260

uccaagaccg acgugagcuc cagcgugauc accucccugg gggccaucgu cagcugcuac     1320

ggcaagacga agugcaccgc cagcaacaag aaccggggga ucaucaagac cuucuccaac     1380

ggcugcgacu acgugagcaa caagggcgug gacaccgugu ccgucgggaa cacccuguac     1440

uacgugaaca agcaggaggg caagagccuc uacgugaagg gggagcccau caucaacuuc     1500

uacgacccgc uggucuuccc cuccgacgag uucgacgcga gcaucuccca ggugaacgag     1560

aagaucaacc agagccuggc cuucauccgc aaguccgacg agcugcucca caacgugaac     1620

gccggcaaga gcacgaccaa caucaugauc accaccauca ucaucgucau caucgugauc     1680

cugcuguccc ucaucgccgu ggggcugcug cucuacugca aggcccggug aggacuagug     1740

gaagcauuuu ccuggcugau uaaaagaaau uacucagcua uggucaucug uuccuguuag     1800

aaggcuaugc agcauauuau auacuaugcg cauguuauga aaugcauaau aaaaaauuuu     1860

aaaaaaucua aaagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa     1980

aggcucuuuu cagagccacc agaauu                                          2006


<210>  180
<211>  1856
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  180
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua guguccaccu gucccuccug ggcugcugga     1680

uugucucguu uuccugccaa auaaacagga ucagcgcuuu acagaucuaa aaaaaaaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc     1800

cccccccccc cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu         1856


<210>  181
<211>  1797
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  181
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggugcccca ggcccuccug uucgucccgc ugcugguguu cccccucugc      120

uucggcaagu uccccaucua caccaucccc gacaagcugg ggccguggag ccccaucgac      180

auccaccacc uguccugccc caacaaccuc guggucgagg acgagggcug caccaaccug      240

agcggguucu ccuacaugga gcugaaggug ggcuacauca gcgccaucaa gaugaacggg      300

uucacgugca ccggcguggu caccgaggcg gagaccuaca cgaacuucgu gggcuacgug      360

accaccaccu ucaagcggaa gcacuuccgc cccacgccgg acgccugccg ggccgccuac      420

aacuggaaga uggccgggga cccccgcuac gaggaguccc uccacaaccc cuaccccgac      480

uaccacuggc ugcggaccgu caagaccacc aaggagagcc uggugaucau cuccccgagc      540

guggcggacc ucgaccccua cgaccgcucc cugcacagcc gggucuuccc cggcgggaac      600

ugcuccggcg uggccgugag cuccacguac ugcagcacca accacgacua caccaucugg      660

augcccgaga acccgcgccu ggggaugucc ugcgacaucu ucaccaacag ccggggcaag      720

cgcgccucca agggcagcga gacgugcggg uucgucgacg agcggggccu cuacaagucc      780

cugaaggggg ccugcaagcu gaagcucugc ggcgugcugg gccugcgccu cauggacggg      840

accugggugg cgaugcagac cagcaacgag accaaguggu gcccccccgg ccagcugguc      900

aaccugcacg acuuccggag cgacgagauc gagcaccucg ugguggagga gcuggucaag      960

aagcgcgagg agugccugga cgcccucgag uccaucauga cgaccaagag cguguccuuc     1020

cggcgccuga gccaccuccg gaagcuggug cccggguucg gcaaggccua caccaucuuc     1080

aacaagaccc ugauggaggc cgacgcccac uacaaguccg uccgcacgug gaacgagauc     1140

aucccgagca aggggugccu gcgggugggc ggccgcugcc acccccacgu caacggggug     1200

uucuucaacg gcaucauccu cgggcccgac ggcaacgugc ugauccccga gaugcagucc     1260

agccugcucc agcagcacau ggagcugcug gucuccagcg ugaucccgcu caugcacccc     1320

cuggcggacc ccuccaccgu guucaagaac ggggacgagg ccgaggacuu cgucgaggug     1380

caccuccccg acgugcacga gcggaucagc ggcgucgacc ugggccugcc gaacuggggg     1440

aaguacgugc ugcucuccgc cggcgcccug accgcccuga ugcugaucau cuuccucaug     1500

accugcuggc gccgggugaa ccggagcgag cccacgcagc acaaccugcg cgggaccggc     1560

cgggaggucu ccgugacccc gcagagcggg aagaucaucu ccagcuggga guccuacaag     1620

agcggcggcg agaccgggcu gugaggacua guguccaccu gucccuccug ggcugcugga     1680

uugucucguu uuccugccaa auaaacagga ucagcgcuuu acagaucuaa aaaaaaaaaa     1740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        1797


<210>  182
<211>  863
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  182
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagug      660

uccaccuguc ccuccugggc ugcuggauug ucucguuuuc cugccaaaua aacaggauca      720

gcgcuuuaca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg      840

cucuuuucag agccaccaga auu                                              863


<210>  183
<211>  804
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  183
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca ugggcgugca cgagugcccc gccuggcugu ggcuccugcu gagccuccug      120

ucccugccgc ucgggcugcc cguccugggc gcccccccgc ggcucaucug cgacagccgc      180

gugcuggagc gguaccugcu cgaggcgaag gaggccgaga acaucaccac cgggugcgcc      240

gagcacugcu cccugaacga gaacaucacg gugccggaca ccaaggucaa cuucuacgcc      300

uggaagcgca uggagguggg ccagcaggcc guggaggucu ggcaggggcu ggcgcuccug      360

agcgaggccg ugcugcgggg ccaggcccuc cuggugaacu ccagccagcc cugggagccc      420

cugcagcucc acgucgacaa ggccgugucc ggccugcgca gccugaccac ccuccugcgg      480

gcgcuggggg cccagaagga ggccaucucc cccccggacg ccgccagcgc ggccccgcuc      540

cgcacgauca ccgccgacac cuuccggaag cuguuccgcg uguacuccaa cuuccugcgg      600

ggcaagcuca agcuguacac cggggaggcc ugccgcacgg gcgaccggug aggacuagug      660

uccaccuguc ccuccugggc ugcuggauug ucucguuuuc cugccaaaua aacaggauca      720

gcgcuuuaca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaag aauu                                             804


<210>  184
<211>  1934
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  184
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu guccaccugu     1740

cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac     1800

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca     1920

gagccaccag aauu                                                       1934


<210>  185
<211>  1875
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  185
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggaggacgc caagaacauc aagaagggcc cggcgcccuu cuacccgcug      120

gaggacggga ccgccggcga gcagcuccac aaggccauga agcgguacgc ccuggugccg      180

ggcacgaucg ccuucaccga cgcccacauc gaggucgaca ucaccuacgc ggaguacuuc      240

gagaugagcg ugcgccuggc cgaggccaug aagcgguacg gccugaacac caaccaccgg      300

aucguggugu gcucggagaa cagccugcag uucuucaugc cggugcuggg cgcccucuuc      360

aucggcgugg ccgucgcccc ggcgaacgac aucuacaacg agcgggagcu gcugaacagc      420

auggggauca gccagccgac cgugguguuc gugagcaaga agggccugca gaagauccug      480

aacgugcaga agaagcugcc caucauccag aagaucauca ucauggacag caagaccgac      540

uaccagggcu uccagucgau guacacguuc gugaccagcc accucccgcc gggcuucaac      600

gaguacgacu ucgucccgga gagcuucgac cgggacaaga ccaucgcccu gaucaugaac      660

agcagcggca gcaccggccu gccgaagggg guggcccugc cgcaccggac cgccugcgug      720

cgcuucucgc acgcccggga ccccaucuuc ggcaaccaga ucaucccgga caccgccauc      780

cugagcgugg ugccguucca ccacggcuuc ggcauguuca cgacccuggg cuaccucauc      840

ugcggcuucc gggugguccu gauguaccgg uucgaggagg agcuguuccu gcggagccug      900

caggacuaca agauccagag cgcgcugcuc gugccgaccc uguucagcuu cuucgccaag      960

agcacccuga ucgacaagua cgaccugucg aaccugcacg agaucgccag cgggggcgcc     1020

ccgcugagca aggagguggg cgaggccgug gccaagcggu uccaccuccc gggcauccgc     1080

cagggcuacg gccugaccga gaccacgagc gcgauccuga ucacccccga gggggacgac     1140

aagccgggcg ccgugggcaa gguggucccg uucuucgagg ccaagguggu ggaccuggac     1200

accggcaaga cccugggcgu gaaccagcgg ggcgagcugu gcgugcgggg gccgaugauc     1260

augagcggcu acgugaacaa cccggaggcc accaacgccc ucaucgacaa ggacggcugg     1320

cugcacagcg gcgacaucgc cuacugggac gaggacgagc acuucuucau cgucgaccgg     1380

cugaagucgc ugaucaagua caagggcuac cagguggcgc cggccgagcu ggagagcauc     1440

cugcuccagc accccaacau cuucgacgcc ggcguggccg ggcugccgga cgacgacgcc     1500

ggcgagcugc cggccgcggu gguggugcug gagcacggca agaccaugac ggagaaggag     1560

aucgucgacu acguggccag ccaggugacc accgccaaga agcugcgggg cggcguggug     1620

uucguggacg aggucccgaa gggccugacc gggaagcucg acgcccggaa gauccgcgag     1680

auccugauca aggccaagaa gggcggcaag aucgccgugu gaggacuagu guccaccugu     1740

cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac     1800

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa gaauu                                                      1875


<210>  186
<211>  1943
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  186
gggagacuuu cccauucugu agcagaauuu gguguugccu guggucuugg ucccgcggag       60

aagcuuacca uggagcugcu cauccugaag gccaacgcca ucaccaccau ccugacggcg      120

gugaccuucu gcuucgccag cggccagaac aucaccgagg aguucuacca guccacgugc      180

agcgccgucu ccaaggggua ccucagcgcc cugcggaccg gcugguacac cuccgugauc      240

accaucgagc ugagcaacau caaggagaac aagugcaacg ggaccgacgc caaggugaag      300

cucaucaagc aggagcugga caaguacaag aacgcgguca cggagcugca gcuccugaug      360

caguccaccc cggcaaccaa caaccgcgcc cggcgcgagc ugccccgguu caugaacuac      420

acccucaaca acgccaagaa gacgaacgug acccugagca agaagcgcaa gcggcgcuuc      480

cugggcuucc uccugggggu gggcuccgcc aucgcgagcg gcgucgccgu gugcaaggug      540

cugcaccucg agggggaggu caacaagauc aagagcgccc ugcuguccac caacaaggcc      600

guggugagcc ucuccaacgg cgucagcgug cugaccuuca aggugcugga ccucaagaac      660

uacaucgaca agcagcugcu gcccauccug aacaagcaga gcugcuccau cagcaacauc      720

gagacgguga ucgaguucca gcagaagaac aaccggcucc uggagaucac ccgcgaguuc      780

agcgugaacg ccggggucac cacccccgug uccacguaca ugcugaccaa cagcgagcuc      840

cugucccuga ucaacgacau gccgaucacc aacgaccaga agaagcucau gagcaacaac      900

gugcagaucg uccggcagca guccuacagc aucaugugca ucaucaagga ggaggugcug      960

gcguacgugg uccagcugcc ccucuacggc gugaucgaca cccccugcug gaagcugcac     1020

acgagccccc ugugcaccac caacaccaag gaggggucca acaucugccu cacgcgcacc     1080

gaccggggcu gguacugcga caacgccggc agcgucuccu ucuucccgca ggccgagacc     1140

ugcaaggugc agagcaaccg cguguucugc gacaccauga acucccugac gcugcccagc     1200

gagguaaacc ucugcaacgu cgacaucuuc aaccccaagu acgacugcaa gaucaugacc     1260

uccaagaccg acgugagcuc cagcgugauc accucccugg gggccaucgu cagcugcuac     1320

ggcaagacga agugcaccgc cagcaacaag aaccggggga ucaucaagac cuucuccaac     1380

ggcugcgacu acgugagcaa caagggcgug gacaccgugu ccgucgggaa cacccuguac     1440

uacgugaaca agcaggaggg caagagccuc uacgugaagg gggagcccau caucaacuuc     1500

uacgacccgc uggucuuccc cuccgacgag uucgacgcga gcaucuccca ggugaacgag     1560

aagaucaacc agagccuggc cuucauccgc aaguccgacg agcugcucca caacgugaac     1620

gccggcaaga gcacgaccaa caucaugauc accaccauca ucaucgucau caucgugauc     1680

cugcuguccc ucaucgccgu ggggcugcug cucuacugca aggcccggug aggacuagug     1740

uccaccuguc ccuccugggc ugcuggauug ucucguuuuc cugccaaaua aacaggauca     1800

gcgcuuuaca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg     1920

cucuuuucag agccaccaga auu                                             1943


<210>  187
<211>  1870
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  187
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuaguc     1680

ccuguuccca gagcccacuu uuuuuucuuu uuuugaaaua aaauagccug ucuuucagau     1740

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1860

caccagaauu                                                            1870


<210>  188
<211>  1811
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  188
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuaguc     1680

ccuguuccca gagcccacuu uuuuuucuuu uuuugaaaua aaauagccug ucuuucagau     1740

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaagaau u                                                          1811


<210>  189
<211>  877
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  189
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu      720

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc      840

ccccccccca aaggcucuuu ucagagccac cagaauu                               877


<210>  190
<211>  818
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  190
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu      720

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                              818


<210>  191
<211>  1948
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  191
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     1800

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     1920

aaaggcucuu uucagagcca ccagaauu                                        1948


<210>  192
<211>  1889
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  192
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     1800

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       1889


<210>  193
<211>  1957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  193
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg agcugcucau ccugaaggcc      120

aacgccauca ccaccauccu gacggcggug accuucugcu ucgccagcgg ccagaacauc      180

accgaggagu ucuaccaguc cacgugcagc gccgucucca agggguaccu cagcgcccug      240

cggaccggcu gguacaccuc cgugaucacc aucgagcuga gcaacaucaa ggagaacaag      300

ugcaacggga ccgacgccaa ggugaagcuc aucaagcagg agcuggacaa guacaagaac      360

gcggucacgg agcugcagcu ccugaugcag uccaccccgg caaccaacaa ccgcgcccgg      420

cgcgagcugc cccgguucau gaacuacacc cucaacaacg ccaagaagac gaacgugacc      480

cugagcaaga agcgcaagcg gcgcuuccug ggcuuccucc uggggguggg cuccgccauc      540

gcgagcggcg ucgccgugug caaggugcug caccucgagg gggaggucaa caagaucaag      600

agcgcccugc uguccaccaa caaggccgug gugagccucu ccaacggcgu cagcgugcug      660

accuucaagg ugcuggaccu caagaacuac aucgacaagc agcugcugcc cauccugaac      720

aagcagagcu gcuccaucag caacaucgag acggugaucg aguuccagca gaagaacaac      780

cggcuccugg agaucacccg cgaguucagc gugaacgccg gggucaccac ccccgugucc      840

acguacaugc ugaccaacag cgagcuccug ucccugauca acgacaugcc gaucaccaac      900

gaccagaaga agcucaugag caacaacgug cagaucgucc ggcagcaguc cuacagcauc      960

augugcauca ucaaggagga ggugcuggcg uacguggucc agcugccccu cuacggcgug     1020

aucgacaccc ccugcuggaa gcugcacacg agcccccugu gcaccaccaa caccaaggag     1080

ggguccaaca ucugccucac gcgcaccgac cggggcuggu acugcgacaa cgccggcagc     1140

gucuccuucu ucccgcaggc cgagaccugc aaggugcaga gcaaccgcgu guucugcgac     1200

accaugaacu cccugacgcu gcccagcgag guaaaccucu gcaacgucga caucuucaac     1260

cccaaguacg acugcaagau caugaccucc aagaccgacg ugagcuccag cgugaucacc     1320

ucccuggggg ccaucgucag cugcuacggc aagacgaagu gcaccgccag caacaagaac     1380

cgggggauca ucaagaccuu cuccaacggc ugcgacuacg ugagcaacaa gggcguggac     1440

accguguccg ucgggaacac ccuguacuac gugaacaagc aggagggcaa gagccucuac     1500

gugaaggggg agcccaucau caacuucuac gacccgcugg ucuuccccuc cgacgaguuc     1560

gacgcgagca ucucccaggu gaacgagaag aucaaccaga gccuggccuu cauccgcaag     1620

uccgacgagc ugcuccacaa cgugaacgcc ggcaagagca cgaccaacau caugaucacc     1680

accaucauca ucgucaucau cgugauccug cugucccuca ucgccguggg gcugcugcuc     1740

uacugcaagg cccggugagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu     1800

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1920

ccccccccca aaggcucuuu ucagagccac cagaauu                              1957


<210>  194
<211>  1934
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  194
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagua     1680

auaaggaaac uguaugaaug ucugugggca ggaagugaag agauccuucu guaaagguuu     1740

uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua auaaaucugg uagaaaaaug     1800

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca     1920

gagccaccag aauu                                                       1934


<210>  195
<211>  1875
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  195
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagua     1680

auaaggaaac uguaugaaug ucugugggca ggaagugaag agauccuucu guaaagguuu     1740

uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua auaaaucugg uagaaaaaug     1800

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa gaauu                                                      1875


<210>  196
<211>  941
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  196
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuaguaaua aggaaacugu augaaugucu gugggcagga      720

agugaagaga uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu      780

ugaaauaaua aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc      900

cccccccccc cccaaaggcu cuuuucagag ccaccagaau u                          941


<210>  197
<211>  882
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  197
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuaguaaua aggaaacugu augaaugucu gugggcagga      720

agugaagaga uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu      780

ugaaauaaua aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                         882


<210>  198
<211>  2012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  198
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag     1800

auccuucugu aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau     1860

aaaucuggua gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc     1980

ccccaaaggc ucuuuucaga gccaccagaa uu                                   2012


<210>  199
<211>  1953
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  199
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag     1800

auccuucugu aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau     1860

aaaucuggua gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  1953


<210>  200
<211>  2021
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  200
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg agcugcucau ccugaaggcc      120

aacgccauca ccaccauccu gacggcggug accuucugcu ucgccagcgg ccagaacauc      180

accgaggagu ucuaccaguc cacgugcagc gccgucucca agggguaccu cagcgcccug      240

cggaccggcu gguacaccuc cgugaucacc aucgagcuga gcaacaucaa ggagaacaag      300

ugcaacggga ccgacgccaa ggugaagcuc aucaagcagg agcuggacaa guacaagaac      360

gcggucacgg agcugcagcu ccugaugcag uccaccccgg caaccaacaa ccgcgcccgg      420

cgcgagcugc cccgguucau gaacuacacc cucaacaacg ccaagaagac gaacgugacc      480

cugagcaaga agcgcaagcg gcgcuuccug ggcuuccucc uggggguggg cuccgccauc      540

gcgagcggcg ucgccgugug caaggugcug caccucgagg gggaggucaa caagaucaag      600

agcgcccugc uguccaccaa caaggccgug gugagccucu ccaacggcgu cagcgugcug      660

accuucaagg ugcuggaccu caagaacuac aucgacaagc agcugcugcc cauccugaac      720

aagcagagcu gcuccaucag caacaucgag acggugaucg aguuccagca gaagaacaac      780

cggcuccugg agaucacccg cgaguucagc gugaacgccg gggucaccac ccccgugucc      840

acguacaugc ugaccaacag cgagcuccug ucccugauca acgacaugcc gaucaccaac      900

gaccagaaga agcucaugag caacaacgug cagaucgucc ggcagcaguc cuacagcauc      960

augugcauca ucaaggagga ggugcuggcg uacguggucc agcugccccu cuacggcgug     1020

aucgacaccc ccugcuggaa gcugcacacg agcccccugu gcaccaccaa caccaaggag     1080

ggguccaaca ucugccucac gcgcaccgac cggggcuggu acugcgacaa cgccggcagc     1140

gucuccuucu ucccgcaggc cgagaccugc aaggugcaga gcaaccgcgu guucugcgac     1200

accaugaacu cccugacgcu gcccagcgag guaaaccucu gcaacgucga caucuucaac     1260

cccaaguacg acugcaagau caugaccucc aagaccgacg ugagcuccag cgugaucacc     1320

ucccuggggg ccaucgucag cugcuacggc aagacgaagu gcaccgccag caacaagaac     1380

cgggggauca ucaagaccuu cuccaacggc ugcgacuacg ugagcaacaa gggcguggac     1440

accguguccg ucgggaacac ccuguacuac gugaacaagc aggagggcaa gagccucuac     1500

gugaaggggg agcccaucau caacuucuac gacccgcugg ucuuccccuc cgacgaguuc     1560

gacgcgagca ucucccaggu gaacgagaag aucaaccaga gccuggccuu cauccgcaag     1620

uccgacgagc ugcuccacaa cgugaacgcc ggcaagagca cgaccaacau caugaucacc     1680

accaucauca ucgucaucau cgugauccug cugucccuca ucgccguggg gcugcugcuc     1740

uacugcaagg cccggugagg acuaguaaua aggaaacugu augaaugucu gugggcagga     1800

agugaagaga uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu     1860

ugaaauaaua aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc     1980

cccccccccc cccaaaggcu cuuuucagag ccaccagaau u                         2021


<210>  201
<211>  1950
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  201
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagua     1680

cuggcugcau cucccuuucc ucuguccucc auccuucucc caggauggug aagggggacc     1740

ugguacccag ugauccccac cccaggaucc uaaaucauga cuuaccugcu aauaaaaacu     1800

cauuggaaaa gugagaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc cccccccccc     1920

ccaaaggcuc uuuucagagc caccagaauu                                      1950


<210>  202
<211>  1891
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  202
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagua     1680

cuggcugcau cucccuuucc ucuguccucc auccuucucc caggauggug aagggggacc     1740

ugguacccag ugauccccac cccaggaucc uaaaucauga cuuaccugcu aauaaaaacu     1800

cauuggaaaa gugagaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                                    1891


<210>  203
<211>  957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  203
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuaguacug gcugcaucuc ccuuuccucu guccuccauc      720

cuucucccag gauggugaag ggggaccugg uacccaguga uccccacccc aggauccuaa      780

aucaugacuu accugcuaau aaaaacucau uggaaaagug agaagaucua aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc      900

cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac cagaauu         957


<210>  204
<211>  898
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  204
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuaguacug gcugcaucuc ccuuuccucu guccuccauc      720

cuucucccag gauggugaag ggggaccugg uacccaguga uccccacccc aggauccuaa      780

aucaugacuu accugcuaau aaaaacucau uggaaaagug agaagaucua aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu        898


<210>  205
<211>  2028
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  205
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuaguacu ggcugcaucu cccuuuccuc uguccuccau ccuucuccca     1800

ggauggugaa gggggaccug guacccagug auccccaccc caggauccua aaucaugacu     1860

uaccugcuaa uaaaaacuca uuggaaaagu gagaagaucu aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc     1980

cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu                  2028


<210>  206
<211>  1969
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  206
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuaguacu ggcugcaucu cccuuuccuc uguccuccau ccuucuccca     1800

ggauggugaa gggggaccug guacccagug auccccaccc caggauccua aaucaugacu     1860

uaccugcuaa uaaaaacuca uuggaaaagu gagaagaucu aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                 1969


<210>  207
<211>  2037
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  207
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg agcugcucau ccugaaggcc      120

aacgccauca ccaccauccu gacggcggug accuucugcu ucgccagcgg ccagaacauc      180

accgaggagu ucuaccaguc cacgugcagc gccgucucca agggguaccu cagcgcccug      240

cggaccggcu gguacaccuc cgugaucacc aucgagcuga gcaacaucaa ggagaacaag      300

ugcaacggga ccgacgccaa ggugaagcuc aucaagcagg agcuggacaa guacaagaac      360

gcggucacgg agcugcagcu ccugaugcag uccaccccgg caaccaacaa ccgcgcccgg      420

cgcgagcugc cccgguucau gaacuacacc cucaacaacg ccaagaagac gaacgugacc      480

cugagcaaga agcgcaagcg gcgcuuccug ggcuuccucc uggggguggg cuccgccauc      540

gcgagcggcg ucgccgugug caaggugcug caccucgagg gggaggucaa caagaucaag      600

agcgcccugc uguccaccaa caaggccgug gugagccucu ccaacggcgu cagcgugcug      660

accuucaagg ugcuggaccu caagaacuac aucgacaagc agcugcugcc cauccugaac      720

aagcagagcu gcuccaucag caacaucgag acggugaucg aguuccagca gaagaacaac      780

cggcuccugg agaucacccg cgaguucagc gugaacgccg gggucaccac ccccgugucc      840

acguacaugc ugaccaacag cgagcuccug ucccugauca acgacaugcc gaucaccaac      900

gaccagaaga agcucaugag caacaacgug cagaucgucc ggcagcaguc cuacagcauc      960

augugcauca ucaaggagga ggugcuggcg uacguggucc agcugccccu cuacggcgug     1020

aucgacaccc ccugcuggaa gcugcacacg agcccccugu gcaccaccaa caccaaggag     1080

ggguccaaca ucugccucac gcgcaccgac cggggcuggu acugcgacaa cgccggcagc     1140

gucuccuucu ucccgcaggc cgagaccugc aaggugcaga gcaaccgcgu guucugcgac     1200

accaugaacu cccugacgcu gcccagcgag guaaaccucu gcaacgucga caucuucaac     1260

cccaaguacg acugcaagau caugaccucc aagaccgacg ugagcuccag cgugaucacc     1320

ucccuggggg ccaucgucag cugcuacggc aagacgaagu gcaccgccag caacaagaac     1380

cgggggauca ucaagaccuu cuccaacggc ugcgacuacg ugagcaacaa gggcguggac     1440

accguguccg ucgggaacac ccuguacuac gugaacaagc aggagggcaa gagccucuac     1500

gugaaggggg agcccaucau caacuucuac gacccgcugg ucuuccccuc cgacgaguuc     1560

gacgcgagca ucucccaggu gaacgagaag aucaaccaga gccuggccuu cauccgcaag     1620

uccgacgagc ugcuccacaa cgugaacgcc ggcaagagca cgaccaacau caugaucacc     1680

accaucauca ucgucaucau cgugauccug cugucccuca ucgccguggg gcugcugcuc     1740

uacugcaagg cccggugagg acuaguacug gcugcaucuc ccuuuccucu guccuccauc     1800

cuucucccag gauggugaag ggggaccugg uacccaguga uccccacccc aggauccuaa     1860

aucaugacuu accugcuaau aaaaacucau uggaaaagug agaagaucua aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc     1980

cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac cagaauu        2037


<210>  208
<211>  2166
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  208
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagug     1680

aagggaacac ccaaauuuaa uucagccuua agcacaauua auuaagagug aaacguaauu     1740

guacaagcag uuggucaccc accauagggc augaucaaca ccgcaaccuu uccuuuuucc     1800

cccagugauu cugaaaaacc ccucuucccu ucagcuugcu uagauguucc aaauuuagua     1860

agcuuaaggc ggccuacaga agaaaaagaa aaaaaaggcc acaaaaguuc ccucucacuu     1920

ucaguaaaua aaauaaaagc agcaacagaa auaaagaaau aaaugaaauu caaaaugaaa     1980

uaaauauugu guugugcagc auuaaaaaau caauaaaaau uaaaaaugag caagaucuaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu cagagccacc     2160

agaauu                                                                2166


<210>  209
<211>  2107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  209
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagug     1680

aagggaacac ccaaauuuaa uucagccuua agcacaauua auuaagagug aaacguaauu     1740

guacaagcag uuggucaccc accauagggc augaucaaca ccgcaaccuu uccuuuuucc     1800

cccagugauu cugaaaaacc ccucuucccu ucagcuugcu uagauguucc aaauuuagua     1860

agcuuaaggc ggccuacaga agaaaaagaa aaaaaaggcc acaaaaguuc ccucucacuu     1920

ucaguaaaua aaauaaaagc agcaacagaa auaaagaaau aaaugaaauu caaaaugaaa     1980

uaaauauugu guugugcagc auuaaaaaau caauaaaaau uaaaaaugag caagaucuaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aagaauu                                                               2107


<210>  210
<211>  1173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  210
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc      720

acaauuaauu aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug      780

aucaacaccg caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca      840

gcuugcuuag auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa      900

aaaggccaca aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua      960

aagaaauaaa ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa     1020

uaaaaauuaa aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc cccccccccc     1140

cccccaaagg cucuuuucag agccaccaga auu                                  1173


<210>  211
<211>  1114
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  211
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc      720

acaauuaauu aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug      780

aucaacaccg caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca      840

gcuugcuuag auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa      900

aaaggccaca aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua      960

aagaaauaaa ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa     1020

uaaaaauuaa aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                                 1114


<210>  212
<211>  2244
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  212
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau     1800

uaagagugaa acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc     1860

gcaaccuuuc cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua     1920

gauguuccaa auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac     1980

aaaaguuccc ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa     2040

augaaauuca aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua     2100

aaaaugagca agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag     2220

gcucuuuuca gagccaccag aauu                                            2244


<210>  213
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  213
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau     1800

uaagagugaa acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc     1860

gcaaccuuuc cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua     1920

gauguuccaa auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac     1980

aaaaguuccc ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa     2040

augaaauuca aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua     2100

aaaaugagca agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa gaauu                                           2185


<210>  214
<211>  2253
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  214
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg agcugcucau ccugaaggcc      120

aacgccauca ccaccauccu gacggcggug accuucugcu ucgccagcgg ccagaacauc      180

accgaggagu ucuaccaguc cacgugcagc gccgucucca agggguaccu cagcgcccug      240

cggaccggcu gguacaccuc cgugaucacc aucgagcuga gcaacaucaa ggagaacaag      300

ugcaacggga ccgacgccaa ggugaagcuc aucaagcagg agcuggacaa guacaagaac      360

gcggucacgg agcugcagcu ccugaugcag uccaccccgg caaccaacaa ccgcgcccgg      420

cgcgagcugc cccgguucau gaacuacacc cucaacaacg ccaagaagac gaacgugacc      480

cugagcaaga agcgcaagcg gcgcuuccug ggcuuccucc uggggguggg cuccgccauc      540

gcgagcggcg ucgccgugug caaggugcug caccucgagg gggaggucaa caagaucaag      600

agcgcccugc uguccaccaa caaggccgug gugagccucu ccaacggcgu cagcgugcug      660

accuucaagg ugcuggaccu caagaacuac aucgacaagc agcugcugcc cauccugaac      720

aagcagagcu gcuccaucag caacaucgag acggugaucg aguuccagca gaagaacaac      780

cggcuccugg agaucacccg cgaguucagc gugaacgccg gggucaccac ccccgugucc      840

acguacaugc ugaccaacag cgagcuccug ucccugauca acgacaugcc gaucaccaac      900

gaccagaaga agcucaugag caacaacgug cagaucgucc ggcagcaguc cuacagcauc      960

augugcauca ucaaggagga ggugcuggcg uacguggucc agcugccccu cuacggcgug     1020

aucgacaccc ccugcuggaa gcugcacacg agcccccugu gcaccaccaa caccaaggag     1080

ggguccaaca ucugccucac gcgcaccgac cggggcuggu acugcgacaa cgccggcagc     1140

gucuccuucu ucccgcaggc cgagaccugc aaggugcaga gcaaccgcgu guucugcgac     1200

accaugaacu cccugacgcu gcccagcgag guaaaccucu gcaacgucga caucuucaac     1260

cccaaguacg acugcaagau caugaccucc aagaccgacg ugagcuccag cgugaucacc     1320

ucccuggggg ccaucgucag cugcuacggc aagacgaagu gcaccgccag caacaagaac     1380

cgggggauca ucaagaccuu cuccaacggc ugcgacuacg ugagcaacaa gggcguggac     1440

accguguccg ucgggaacac ccuguacuac gugaacaagc aggagggcaa gagccucuac     1500

gugaaggggg agcccaucau caacuucuac gacccgcugg ucuuccccuc cgacgaguuc     1560

gacgcgagca ucucccaggu gaacgagaag aucaaccaga gccuggccuu cauccgcaag     1620

uccgacgagc ugcuccacaa cgugaacgcc ggcaagagca cgaccaacau caugaucacc     1680

accaucauca ucgucaucau cgugauccug cugucccuca ucgccguggg gcugcugcuc     1740

uacugcaagg cccggugagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc     1800

acaauuaauu aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug     1860

aucaacaccg caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca     1920

gcuugcuuag auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa     1980

aaaggccaca aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua     2040

aagaaauaaa ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa     2100

uaaaaauuaa aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc cccccccccc     2220

cccccaaagg cucuuuucag agccaccaga auu                                  2253


<210>  215
<211>  1946
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  215
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagug     1680

gaagcauuuu ccuggcugau uaaaagaaau uacucagcua uggucaucug uuccuguuag     1740

aaggcuaugc agcauauuau auacuaugcg cauguuauga aaugcauaau aaaaaauuuu     1800

aaaaaaucua aaagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa     1920

aggcucuuuu cagagccacc agaauu                                          1946


<210>  216
<211>  1887
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  216
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagug     1680

gaagcauuuu ccuggcugau uaaaagaaau uacucagcua uggucaucug uuccuguuag     1740

aaggcuaugc agcauauuau auacuaugcg cauguuauga aaugcauaau aaaaaauuuu     1800

aaaaaaucua aaagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aagaauu                                         1887


<210>  217
<211>  953
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  217
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuaguggaa gcauuuuccu ggcugauuaa aagaaauuac      720

ucagcuaugg ucaucuguuc cuguuagaag gcuaugcagc auauuauaua cuaugcgcau      780

guuaugaaau gcauaauaaa aaauuuuaaa aaaucuaaaa gaucuaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc      900

cccccccccc cccccccccc cccccaaagg cucuuuucag agccaccaga auu             953


<210>  218
<211>  894
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  218
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuaguggaa gcauuuuccu ggcugauuaa aagaaauuac      720

ucagcuaugg ucaucuguuc cuguuagaag gcuaugcagc auauuauaua cuaugcgcau      780

guuaugaaau gcauaauaaa aaauuuuaaa aaaucuaaaa gaucuaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu            894


<210>  219
<211>  2024
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  219
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuagugga agcauuuucc uggcugauua aaagaaauua cucagcuaug     1800

gucaucuguu ccuguuagaa ggcuaugcag cauauuauau acuaugcgca uguuaugaaa     1860

ugcauaauaa aaaauuuuaa aaaaucuaaa agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc     1980

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                      2024


<210>  220
<211>  1965
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  220
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuagugga agcauuuucc uggcugauua aaagaaauua cucagcuaug     1800

gucaucuguu ccuguuagaa ggcuaugcag cauauuauau acuaugcgca uguuaugaaa     1860

ugcauaauaa aaaauuuuaa aaaaucuaaa agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     1965


<210>  221
<211>  2033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  221
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg agcugcucau ccugaaggcc      120

aacgccauca ccaccauccu gacggcggug accuucugcu ucgccagcgg ccagaacauc      180

accgaggagu ucuaccaguc cacgugcagc gccgucucca agggguaccu cagcgcccug      240

cggaccggcu gguacaccuc cgugaucacc aucgagcuga gcaacaucaa ggagaacaag      300

ugcaacggga ccgacgccaa ggugaagcuc aucaagcagg agcuggacaa guacaagaac      360

gcggucacgg agcugcagcu ccugaugcag uccaccccgg caaccaacaa ccgcgcccgg      420

cgcgagcugc cccgguucau gaacuacacc cucaacaacg ccaagaagac gaacgugacc      480

cugagcaaga agcgcaagcg gcgcuuccug ggcuuccucc uggggguggg cuccgccauc      540

gcgagcggcg ucgccgugug caaggugcug caccucgagg gggaggucaa caagaucaag      600

agcgcccugc uguccaccaa caaggccgug gugagccucu ccaacggcgu cagcgugcug      660

accuucaagg ugcuggaccu caagaacuac aucgacaagc agcugcugcc cauccugaac      720

aagcagagcu gcuccaucag caacaucgag acggugaucg aguuccagca gaagaacaac      780

cggcuccugg agaucacccg cgaguucagc gugaacgccg gggucaccac ccccgugucc      840

acguacaugc ugaccaacag cgagcuccug ucccugauca acgacaugcc gaucaccaac      900

gaccagaaga agcucaugag caacaacgug cagaucgucc ggcagcaguc cuacagcauc      960

augugcauca ucaaggagga ggugcuggcg uacguggucc agcugccccu cuacggcgug     1020

aucgacaccc ccugcuggaa gcugcacacg agcccccugu gcaccaccaa caccaaggag     1080

ggguccaaca ucugccucac gcgcaccgac cggggcuggu acugcgacaa cgccggcagc     1140

gucuccuucu ucccgcaggc cgagaccugc aaggugcaga gcaaccgcgu guucugcgac     1200

accaugaacu cccugacgcu gcccagcgag guaaaccucu gcaacgucga caucuucaac     1260

cccaaguacg acugcaagau caugaccucc aagaccgacg ugagcuccag cgugaucacc     1320

ucccuggggg ccaucgucag cugcuacggc aagacgaagu gcaccgccag caacaagaac     1380

cgggggauca ucaagaccuu cuccaacggc ugcgacuacg ugagcaacaa gggcguggac     1440

accguguccg ucgggaacac ccuguacuac gugaacaagc aggagggcaa gagccucuac     1500

gugaaggggg agcccaucau caacuucuac gacccgcugg ucuuccccuc cgacgaguuc     1560

gacgcgagca ucucccaggu gaacgagaag aucaaccaga gccuggccuu cauccgcaag     1620

uccgacgagc ugcuccacaa cgugaacgcc ggcaagagca cgaccaacau caugaucacc     1680

accaucauca ucgucaucau cgugauccug cugucccuca ucgccguggg gcugcugcuc     1740

uacugcaagg cccggugagg acuaguggaa gcauuuuccu ggcugauuaa aagaaauuac     1800

ucagcuaugg ucaucuguuc cuguuagaag gcuaugcagc auauuauaua cuaugcgcau     1860

guuaugaaau gcauaauaaa aaauuuuaaa aaaucuaaaa gaucuaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc     1980

cccccccccc cccccccccc cccccaaagg cucuuuucag agccaccaga auu            2033


<210>  222
<211>  1883
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  222
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagug     1680

uccaccuguc ccuccugggc ugcuggauug ucucguuuuc cugccaaaua aacaggauca     1740

gcgcuuuaca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg     1860

cucuuuucag agccaccaga auu                                             1883


<210>  223
<211>  1824
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  223
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ugccccaggc ccuccuguuc      120

gucccgcugc ugguguuccc ccucugcuuc ggcaaguucc ccaucuacac cauccccgac      180

aagcuggggc cguggagccc caucgacauc caccaccugu ccugccccaa caaccucgug      240

gucgaggacg agggcugcac caaccugagc ggguucuccu acauggagcu gaaggugggc      300

uacaucagcg ccaucaagau gaacggguuc acgugcaccg gcguggucac cgaggcggag      360

accuacacga acuucguggg cuacgugacc accaccuuca agcggaagca cuuccgcccc      420

acgccggacg ccugccgggc cgccuacaac uggaagaugg ccggggaccc ccgcuacgag      480

gagucccucc acaaccccua ccccgacuac cacuggcugc ggaccgucaa gaccaccaag      540

gagagccugg ugaucaucuc cccgagcgug gcggaccucg accccuacga ccgcucccug      600

cacagccggg ucuuccccgg cgggaacugc uccggcgugg ccgugagcuc cacguacugc      660

agcaccaacc acgacuacac caucuggaug cccgagaacc cgcgccuggg gauguccugc      720

gacaucuuca ccaacagccg gggcaagcgc gccuccaagg gcagcgagac gugcggguuc      780

gucgacgagc ggggccucua caagucccug aagggggccu gcaagcugaa gcucugcggc      840

gugcugggcc ugcgccucau ggacgggacc uggguggcga ugcagaccag caacgagacc      900

aaguggugcc cccccggcca gcuggucaac cugcacgacu uccggagcga cgagaucgag      960

caccucgugg uggaggagcu ggucaagaag cgcgaggagu gccuggacgc ccucgagucc     1020

aucaugacga ccaagagcgu guccuuccgg cgccugagcc accuccggaa gcuggugccc     1080

ggguucggca aggccuacac caucuucaac aagacccuga uggaggccga cgcccacuac     1140

aaguccgucc gcacguggaa cgagaucauc ccgagcaagg ggugccugcg ggugggcggc     1200

cgcugccacc cccacgucaa cgggguguuc uucaacggca ucauccucgg gcccgacggc     1260

aacgugcuga uccccgagau gcaguccagc cugcuccagc agcacaugga gcugcugguc     1320

uccagcguga ucccgcucau gcacccccug gcggaccccu ccaccguguu caagaacggg     1380

gacgaggccg aggacuucgu cgaggugcac cuccccgacg ugcacgagcg gaucagcggc     1440

gucgaccugg gccugccgaa cugggggaag uacgugcugc ucuccgccgg cgcccugacc     1500

gcccugaugc ugaucaucuu ccucaugacc ugcuggcgcc gggugaaccg gagcgagccc     1560

acgcagcaca accugcgcgg gaccggccgg gaggucuccg ugaccccgca gagcgggaag     1620

aucaucucca gcugggaguc cuacaagagc ggcggcgaga ccgggcugug aggacuagug     1680

uccaccuguc ccuccugggc ugcuggauug ucucguuuuc cugccaaaua aacaggauca     1740

gcgcuuuaca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaag aauu                                            1824


<210>  224
<211>  890
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  224
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuagugucc accugucccu ccugggcugc uggauugucu      720

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc      840

cccccccccc cccccccccc ccaaaggcuc uuuucagagc caccagaauu                 890


<210>  225
<211>  831
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  225
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg gcgugcacga gugccccgcc      120

uggcuguggc uccugcugag ccuccugucc cugccgcucg ggcugcccgu ccugggcgcc      180

cccccgcggc ucaucugcga cagccgcgug cuggagcggu accugcucga ggcgaaggag      240

gccgagaaca ucaccaccgg gugcgccgag cacugcuccc ugaacgagaa caucacggug      300

ccggacacca aggucaacuu cuacgccugg aagcgcaugg aggugggcca gcaggccgug      360

gaggucuggc aggggcuggc gcuccugagc gaggccgugc ugcggggcca ggcccuccug      420

gugaacucca gccagcccug ggagccccug cagcuccacg ucgacaaggc cguguccggc      480

cugcgcagcc ugaccacccu ccugcgggcg cugggggccc agaaggaggc caucuccccc      540

ccggacgccg ccagcgcggc cccgcuccgc acgaucaccg ccgacaccuu ccggaagcug      600

uuccgcgugu acuccaacuu ccugcggggc aagcucaagc uguacaccgg ggaggccugc      660

cgcacgggcg accggugagg acuagugucc accugucccu ccugggcugc uggauugucu      720

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u               831


<210>  226
<211>  1961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  226
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu     1800

gccaaauaaa caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc     1920

cccccccccc cccaaaggcu cuuuucagag ccaccagaau u                         1961


<210>  227
<211>  1902
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  227
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg aggacgccaa gaacaucaag      120

aagggcccgg cgcccuucua cccgcuggag gacgggaccg ccggcgagca gcuccacaag      180

gccaugaagc gguacgcccu ggugccgggc acgaucgccu ucaccgacgc ccacaucgag      240

gucgacauca ccuacgcgga guacuucgag augagcgugc gccuggccga ggccaugaag      300

cgguacggcc ugaacaccaa ccaccggauc guggugugcu cggagaacag ccugcaguuc      360

uucaugccgg ugcugggcgc ccucuucauc ggcguggccg ucgccccggc gaacgacauc      420

uacaacgagc gggagcugcu gaacagcaug gggaucagcc agccgaccgu gguguucgug      480

agcaagaagg gccugcagaa gauccugaac gugcagaaga agcugcccau cauccagaag      540

aucaucauca uggacagcaa gaccgacuac cagggcuucc agucgaugua cacguucgug      600

accagccacc ucccgccggg cuucaacgag uacgacuucg ucccggagag cuucgaccgg      660

gacaagacca ucgcccugau caugaacagc agcggcagca ccggccugcc gaagggggug      720

gcccugccgc accggaccgc cugcgugcgc uucucgcacg cccgggaccc caucuucggc      780

aaccagauca ucccggacac cgccauccug agcguggugc cguuccacca cggcuucggc      840

auguucacga cccugggcua ccucaucugc ggcuuccggg ugguccugau guaccgguuc      900

gaggaggagc uguuccugcg gagccugcag gacuacaaga uccagagcgc gcugcucgug      960

ccgacccugu ucagcuucuu cgccaagagc acccugaucg acaaguacga ccugucgaac     1020

cugcacgaga ucgccagcgg gggcgccccg cugagcaagg aggugggcga ggccguggcc     1080

aagcgguucc accucccggg cauccgccag ggcuacggcc ugaccgagac cacgagcgcg     1140

auccugauca cccccgaggg ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc     1200

uucgaggcca agguggugga ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc     1260

gagcugugcg ugcgggggcc gaugaucaug agcggcuacg ugaacaaccc ggaggccacc     1320

aacgcccuca ucgacaagga cggcuggcug cacagcggcg acaucgccua cugggacgag     1380

gacgagcacu ucuucaucgu cgaccggcug aagucgcuga ucaaguacaa gggcuaccag     1440

guggcgccgg ccgagcugga gagcauccug cuccagcacc ccaacaucuu cgacgccggc     1500

guggccgggc ugccggacga cgacgccggc gagcugccgg ccgcgguggu ggugcuggag     1560

cacggcaaga ccaugacgga gaaggagauc gucgacuacg uggccagcca ggugaccacc     1620

gccaagaagc ugcggggcgg cgugguguuc guggacgagg ucccgaaggg ccugaccggg     1680

aagcucgacg cccggaagau ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc     1740

gccgugugag gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu     1800

gccaaauaaa caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                        1902


<210>  228
<211>  1970
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  228
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg agcugcucau ccugaaggcc      120

aacgccauca ccaccauccu gacggcggug accuucugcu ucgccagcgg ccagaacauc      180

accgaggagu ucuaccaguc cacgugcagc gccgucucca agggguaccu cagcgcccug      240

cggaccggcu gguacaccuc cgugaucacc aucgagcuga gcaacaucaa ggagaacaag      300

ugcaacggga ccgacgccaa ggugaagcuc aucaagcagg agcuggacaa guacaagaac      360

gcggucacgg agcugcagcu ccugaugcag uccaccccgg caaccaacaa ccgcgcccgg      420

cgcgagcugc cccgguucau gaacuacacc cucaacaacg ccaagaagac gaacgugacc      480

cugagcaaga agcgcaagcg gcgcuuccug ggcuuccucc uggggguggg cuccgccauc      540

gcgagcggcg ucgccgugug caaggugcug caccucgagg gggaggucaa caagaucaag      600

agcgcccugc uguccaccaa caaggccgug gugagccucu ccaacggcgu cagcgugcug      660

accuucaagg ugcuggaccu caagaacuac aucgacaagc agcugcugcc cauccugaac      720

aagcagagcu gcuccaucag caacaucgag acggugaucg aguuccagca gaagaacaac      780

cggcuccugg agaucacccg cgaguucagc gugaacgccg gggucaccac ccccgugucc      840

acguacaugc ugaccaacag cgagcuccug ucccugauca acgacaugcc gaucaccaac      900

gaccagaaga agcucaugag caacaacgug cagaucgucc ggcagcaguc cuacagcauc      960

augugcauca ucaaggagga ggugcuggcg uacguggucc agcugccccu cuacggcgug     1020

aucgacaccc ccugcuggaa gcugcacacg agcccccugu gcaccaccaa caccaaggag     1080

ggguccaaca ucugccucac gcgcaccgac cggggcuggu acugcgacaa cgccggcagc     1140

gucuccuucu ucccgcaggc cgagaccugc aaggugcaga gcaaccgcgu guucugcgac     1200

accaugaacu cccugacgcu gcccagcgag guaaaccucu gcaacgucga caucuucaac     1260

cccaaguacg acugcaagau caugaccucc aagaccgacg ugagcuccag cgugaucacc     1320

ucccuggggg ccaucgucag cugcuacggc aagacgaagu gcaccgccag caacaagaac     1380

cgggggauca ucaagaccuu cuccaacggc ugcgacuacg ugagcaacaa gggcguggac     1440

accguguccg ucgggaacac ccuguacuac gugaacaagc aggagggcaa gagccucuac     1500

gugaaggggg agcccaucau caacuucuac gacccgcugg ucuuccccuc cgacgaguuc     1560

gacgcgagca ucucccaggu gaacgagaag aucaaccaga gccuggccuu cauccgcaag     1620

uccgacgagc ugcuccacaa cgugaacgcc ggcaagagca cgaccaacau caugaucacc     1680

accaucauca ucgucaucau cgugauccug cugucccuca ucgccguggg gcugcugcuc     1740

uacugcaagg cccggugagg acuagugucc accugucccu ccugggcugc uggauugucu     1800

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc     1920

cccccccccc cccccccccc ccaaaggcuc uuuucagagc caccagaauu                1970


<210>  229
<211>  1897
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  229
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu     1740

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1860

ccccccccca aaggcucuuu ucagagccac cagaauu                              1897


<210>  230
<211>  1838
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  230
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu     1740

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                             1838


<210>  231
<211>  904
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  231
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu agucccuguu      720

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag      900

aauu                                                                   904


<210>  232
<211>  845
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  232
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu agucccuguu      720

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

gaauu                                                                  845


<210>  233
<211>  1975
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  233
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uagucccugu ucccagagcc     1800

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     1920

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          1975


<210>  234
<211>  1916
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  234
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uagucccugu ucccagagcc     1800

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         1916


<210>  235
<211>  1984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  235
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagc ugcucauccu gaaggccaac gccaucacca ccauccugac ggcggugacc      180

uucugcuucg ccagcggcca gaacaucacc gaggaguucu accaguccac gugcagcgcc      240

gucuccaagg gguaccucag cgcccugcgg accggcuggu acaccuccgu gaucaccauc      300

gagcugagca acaucaagga gaacaagugc aacgggaccg acgccaaggu gaagcucauc      360

aagcaggagc uggacaagua caagaacgcg gucacggagc ugcagcuccu gaugcagucc      420

accccggcaa ccaacaaccg cgcccggcgc gagcugcccc gguucaugaa cuacacccuc      480

aacaacgcca agaagacgaa cgugacccug agcaagaagc gcaagcggcg cuuccugggc      540

uuccuccugg gggugggcuc cgccaucgcg agcggcgucg ccgugugcaa ggugcugcac      600

cucgaggggg aggucaacaa gaucaagagc gcccugcugu ccaccaacaa ggccguggug      660

agccucucca acggcgucag cgugcugacc uucaaggugc uggaccucaa gaacuacauc      720

gacaagcagc ugcugcccau ccugaacaag cagagcugcu ccaucagcaa caucgagacg      780

gugaucgagu uccagcagaa gaacaaccgg cuccuggaga ucacccgcga guucagcgug      840

aacgccgggg ucaccacccc cguguccacg uacaugcuga ccaacagcga gcuccugucc      900

cugaucaacg acaugccgau caccaacgac cagaagaagc ucaugagcaa caacgugcag      960

aucguccggc agcaguccua cagcaucaug ugcaucauca aggaggaggu gcuggcguac     1020

gugguccagc ugccccucua cggcgugauc gacacccccu gcuggaagcu gcacacgagc     1080

ccccugugca ccaccaacac caaggagggg uccaacaucu gccucacgcg caccgaccgg     1140

ggcugguacu gcgacaacgc cggcagcguc uccuucuucc cgcaggccga gaccugcaag     1200

gugcagagca accgcguguu cugcgacacc augaacuccc ugacgcugcc cagcgaggua     1260

aaccucugca acgucgacau cuucaacccc aaguacgacu gcaagaucau gaccuccaag     1320

accgacguga gcuccagcgu gaucaccucc cugggggcca ucgucagcug cuacggcaag     1380

acgaagugca ccgccagcaa caagaaccgg gggaucauca agaccuucuc caacggcugc     1440

gacuacguga gcaacaaggg cguggacacc guguccgucg ggaacacccu guacuacgug     1500

aacaagcagg agggcaagag ccucuacgug aagggggagc ccaucaucaa cuucuacgac     1560

ccgcuggucu uccccuccga cgaguucgac gcgagcaucu cccaggugaa cgagaagauc     1620

aaccagagcc uggccuucau ccgcaagucc gacgagcugc uccacaacgu gaacgccggc     1680

aagagcacga ccaacaucau gaucaccacc aucaucaucg ucaucaucgu gauccugcug     1740

ucccucaucg ccguggggcu gcugcucuac ugcaaggccc ggugaggacu agucccuguu     1800

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1980

aauu                                                                  1984


<210>  236
<211>  1961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  236
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuaguaaua aggaaacugu augaaugucu gugggcagga     1740

agugaagaga uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu     1800

ugaaauaaua aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc     1920

cccccccccc cccaaaggcu cuuuucagag ccaccagaau u                         1961


<210>  237
<211>  1902
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  237
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuaguaaua aggaaacugu augaaugucu gugggcagga     1740

agugaagaga uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu     1800

ugaaauaaua aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                        1902


<210>  238
<211>  968
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  238
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu aguaauaagg      720

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa      780

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca      960

ccagaauu                                                               968


<210>  239
<211>  909
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  239
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu aguaauaagg      720

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa      780

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaagaauu                                                              909


<210>  240
<211>  2039
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  240
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uaguaauaag gaaacuguau     1800

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     1860

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau     1980

cccccccccc cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu      2039


<210>  241
<211>  1980
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  241
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uaguaauaag gaaacuguau     1800

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     1860

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     1980


<210>  242
<211>  2048
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  242
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagc ugcucauccu gaaggccaac gccaucacca ccauccugac ggcggugacc      180

uucugcuucg ccagcggcca gaacaucacc gaggaguucu accaguccac gugcagcgcc      240

gucuccaagg gguaccucag cgcccugcgg accggcuggu acaccuccgu gaucaccauc      300

gagcugagca acaucaagga gaacaagugc aacgggaccg acgccaaggu gaagcucauc      360

aagcaggagc uggacaagua caagaacgcg gucacggagc ugcagcuccu gaugcagucc      420

accccggcaa ccaacaaccg cgcccggcgc gagcugcccc gguucaugaa cuacacccuc      480

aacaacgcca agaagacgaa cgugacccug agcaagaagc gcaagcggcg cuuccugggc      540

uuccuccugg gggugggcuc cgccaucgcg agcggcgucg ccgugugcaa ggugcugcac      600

cucgaggggg aggucaacaa gaucaagagc gcccugcugu ccaccaacaa ggccguggug      660

agccucucca acggcgucag cgugcugacc uucaaggugc uggaccucaa gaacuacauc      720

gacaagcagc ugcugcccau ccugaacaag cagagcugcu ccaucagcaa caucgagacg      780

gugaucgagu uccagcagaa gaacaaccgg cuccuggaga ucacccgcga guucagcgug      840

aacgccgggg ucaccacccc cguguccacg uacaugcuga ccaacagcga gcuccugucc      900

cugaucaacg acaugccgau caccaacgac cagaagaagc ucaugagcaa caacgugcag      960

aucguccggc agcaguccua cagcaucaug ugcaucauca aggaggaggu gcuggcguac     1020

gugguccagc ugccccucua cggcgugauc gacacccccu gcuggaagcu gcacacgagc     1080

ccccugugca ccaccaacac caaggagggg uccaacaucu gccucacgcg caccgaccgg     1140

ggcugguacu gcgacaacgc cggcagcguc uccuucuucc cgcaggccga gaccugcaag     1200

gugcagagca accgcguguu cugcgacacc augaacuccc ugacgcugcc cagcgaggua     1260

aaccucugca acgucgacau cuucaacccc aaguacgacu gcaagaucau gaccuccaag     1320

accgacguga gcuccagcgu gaucaccucc cugggggcca ucgucagcug cuacggcaag     1380

acgaagugca ccgccagcaa caagaaccgg gggaucauca agaccuucuc caacggcugc     1440

gacuacguga gcaacaaggg cguggacacc guguccgucg ggaacacccu guacuacgug     1500

aacaagcagg agggcaagag ccucuacgug aagggggagc ccaucaucaa cuucuacgac     1560

ccgcuggucu uccccuccga cgaguucgac gcgagcaucu cccaggugaa cgagaagauc     1620

aaccagagcc uggccuucau ccgcaagucc gacgagcugc uccacaacgu gaacgccggc     1680

aagagcacga ccaacaucau gaucaccacc aucaucaucg ucaucaucgu gauccugcug     1740

ucccucaucg ccguggggcu gcugcucuac ugcaaggccc ggugaggacu aguaauaagg     1800

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa     1860

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca     2040

ccagaauu                                                              2048


<210>  243
<211>  1977
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  243
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuaguacug gcugcaucuc ccuuuccucu guccuccauc     1740

cuucucccag gauggugaag ggggaccugg uacccaguga uccccacccc aggauccuaa     1800

aucaugacuu accugcuaau aaaaacucau uggaaaagug agaagaucua aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc     1920

cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac cagaauu        1977


<210>  244
<211>  1918
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  244
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuaguacug gcugcaucuc ccuuuccucu guccuccauc     1740

cuucucccag gauggugaag ggggaccugg uacccaguga uccccacccc aggauccuaa     1800

aucaugacuu accugcuaau aaaaacucau uggaaaagug agaagaucua aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu       1918


<210>  245
<211>  984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  245
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu aguacuggcu      720

gcaucucccu uuccucuguc cuccauccuu cucccaggau ggugaagggg gaccugguac      780

ccagugaucc ccaccccagg auccuaaauc augacuuacc ugcuaauaaa aacucauugg      840

aaaagugaga agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag      960

gcucuuuuca gagccaccag aauu                                             984


<210>  246
<211>  925
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  246
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu aguacuggcu      720

gcaucucccu uuccucuguc cuccauccuu cucccaggau ggugaagggg gaccugguac      780

ccagugaucc ccaccccagg auccuaaauc augacuuacc ugcuaauaaa aacucauugg      840

aaaagugaga agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaaaaaaa aaaaaaaaaa gaauu                                            925


<210>  247
<211>  2055
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  247
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uaguacuggc ugcaucuccc     1800

uuuccucugu ccuccauccu ucucccagga uggugaaggg ggaccuggua cccagugauc     1860

cccaccccag gauccuaaau caugacuuac cugcuaauaa aaacucauug gaaaagugag     1920

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa ggcucuuuuc     2040

agagccacca gaauu                                                      2055


<210>  248
<211>  1996
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  248
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uaguacuggc ugcaucuccc     1800

uuuccucugu ccuccauccu ucucccagga uggugaaggg ggaccuggua cccagugauc     1860

cccaccccag gauccuaaau caugacuuac cugcuaauaa aaacucauug gaaaagugag     1920

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaaaaaaa agaauu                                                     1996


<210>  249
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  249
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagc ugcucauccu gaaggccaac gccaucacca ccauccugac ggcggugacc      180

uucugcuucg ccagcggcca gaacaucacc gaggaguucu accaguccac gugcagcgcc      240

gucuccaagg gguaccucag cgcccugcgg accggcuggu acaccuccgu gaucaccauc      300

gagcugagca acaucaagga gaacaagugc aacgggaccg acgccaaggu gaagcucauc      360

aagcaggagc uggacaagua caagaacgcg gucacggagc ugcagcuccu gaugcagucc      420

accccggcaa ccaacaaccg cgcccggcgc gagcugcccc gguucaugaa cuacacccuc      480

aacaacgcca agaagacgaa cgugacccug agcaagaagc gcaagcggcg cuuccugggc      540

uuccuccugg gggugggcuc cgccaucgcg agcggcgucg ccgugugcaa ggugcugcac      600

cucgaggggg aggucaacaa gaucaagagc gcccugcugu ccaccaacaa ggccguggug      660

agccucucca acggcgucag cgugcugacc uucaaggugc uggaccucaa gaacuacauc      720

gacaagcagc ugcugcccau ccugaacaag cagagcugcu ccaucagcaa caucgagacg      780

gugaucgagu uccagcagaa gaacaaccgg cuccuggaga ucacccgcga guucagcgug      840

aacgccgggg ucaccacccc cguguccacg uacaugcuga ccaacagcga gcuccugucc      900

cugaucaacg acaugccgau caccaacgac cagaagaagc ucaugagcaa caacgugcag      960

aucguccggc agcaguccua cagcaucaug ugcaucauca aggaggaggu gcuggcguac     1020

gugguccagc ugccccucua cggcgugauc gacacccccu gcuggaagcu gcacacgagc     1080

ccccugugca ccaccaacac caaggagggg uccaacaucu gccucacgcg caccgaccgg     1140

ggcugguacu gcgacaacgc cggcagcguc uccuucuucc cgcaggccga gaccugcaag     1200

gugcagagca accgcguguu cugcgacacc augaacuccc ugacgcugcc cagcgaggua     1260

aaccucugca acgucgacau cuucaacccc aaguacgacu gcaagaucau gaccuccaag     1320

accgacguga gcuccagcgu gaucaccucc cugggggcca ucgucagcug cuacggcaag     1380

acgaagugca ccgccagcaa caagaaccgg gggaucauca agaccuucuc caacggcugc     1440

gacuacguga gcaacaaggg cguggacacc guguccgucg ggaacacccu guacuacgug     1500

aacaagcagg agggcaagag ccucuacgug aagggggagc ccaucaucaa cuucuacgac     1560

ccgcuggucu uccccuccga cgaguucgac gcgagcaucu cccaggugaa cgagaagauc     1620

aaccagagcc uggccuucau ccgcaagucc gacgagcugc uccacaacgu gaacgccggc     1680

aagagcacga ccaacaucau gaucaccacc aucaucaucg ucaucaucgu gauccugcug     1740

ucccucaucg ccguggggcu gcugcucuac ugcaaggccc ggugaggacu aguacuggcu     1800

gcaucucccu uuccucuguc cuccauccuu cucccaggau ggugaagggg gaccugguac     1860

ccagugaucc ccaccccagg auccuaaauc augacuuacc ugcuaauaaa aacucauugg     1920

aaaagugaga agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag     2040

gcucuuuuca gagccaccag aauu                                            2064


<210>  250
<211>  2193
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  250
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc     1740

acaauuaauu aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug     1800

aucaacaccg caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca     1860

gcuugcuuag auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa     1920

aaaggccaca aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua     1980

aagaaauaaa ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa     2040

uaaaaauuaa aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc cccccccccc     2160

cccccaaagg cucuuuucag agccaccaga auu                                  2193


<210>  251
<211>  2134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  251
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc     1740

acaauuaauu aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug     1800

aucaacaccg caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca     1860

gcuugcuuag auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa     1920

aaaggccaca aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua     1980

aagaaauaaa ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa     2040

uaaaaauuaa aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                                 2134


<210>  252
<211>  1200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  252
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu agugaaggga      720

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa      780

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu      840

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua      900

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua      960

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     1020

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     1080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca     1140

uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc caccagaauu     1200


<210>  253
<211>  1141
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  253
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu agugaaggga      720

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa      780

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu      840

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua      900

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua      960

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     1020

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     1080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau     1140

u                                                                     1141


<210>  254
<211>  2271
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  254
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uagugaaggg aacacccaaa     1800

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu     1860

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa     1920

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu     1980

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     2040

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     2100

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc     2220

cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau u              2271


<210>  255
<211>  2212
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  255
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uagugaaggg aacacccaaa     1800

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu     1860

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa     1920

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu     1980

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     2040

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     2100

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             2212


<210>  256
<211>  2280
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  256
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagc ugcucauccu gaaggccaac gccaucacca ccauccugac ggcggugacc      180

uucugcuucg ccagcggcca gaacaucacc gaggaguucu accaguccac gugcagcgcc      240

gucuccaagg gguaccucag cgcccugcgg accggcuggu acaccuccgu gaucaccauc      300

gagcugagca acaucaagga gaacaagugc aacgggaccg acgccaaggu gaagcucauc      360

aagcaggagc uggacaagua caagaacgcg gucacggagc ugcagcuccu gaugcagucc      420

accccggcaa ccaacaaccg cgcccggcgc gagcugcccc gguucaugaa cuacacccuc      480

aacaacgcca agaagacgaa cgugacccug agcaagaagc gcaagcggcg cuuccugggc      540

uuccuccugg gggugggcuc cgccaucgcg agcggcgucg ccgugugcaa ggugcugcac      600

cucgaggggg aggucaacaa gaucaagagc gcccugcugu ccaccaacaa ggccguggug      660

agccucucca acggcgucag cgugcugacc uucaaggugc uggaccucaa gaacuacauc      720

gacaagcagc ugcugcccau ccugaacaag cagagcugcu ccaucagcaa caucgagacg      780

gugaucgagu uccagcagaa gaacaaccgg cuccuggaga ucacccgcga guucagcgug      840

aacgccgggg ucaccacccc cguguccacg uacaugcuga ccaacagcga gcuccugucc      900

cugaucaacg acaugccgau caccaacgac cagaagaagc ucaugagcaa caacgugcag      960

aucguccggc agcaguccua cagcaucaug ugcaucauca aggaggaggu gcuggcguac     1020

gugguccagc ugccccucua cggcgugauc gacacccccu gcuggaagcu gcacacgagc     1080

ccccugugca ccaccaacac caaggagggg uccaacaucu gccucacgcg caccgaccgg     1140

ggcugguacu gcgacaacgc cggcagcguc uccuucuucc cgcaggccga gaccugcaag     1200

gugcagagca accgcguguu cugcgacacc augaacuccc ugacgcugcc cagcgaggua     1260

aaccucugca acgucgacau cuucaacccc aaguacgacu gcaagaucau gaccuccaag     1320

accgacguga gcuccagcgu gaucaccucc cugggggcca ucgucagcug cuacggcaag     1380

acgaagugca ccgccagcaa caagaaccgg gggaucauca agaccuucuc caacggcugc     1440

gacuacguga gcaacaaggg cguggacacc guguccgucg ggaacacccu guacuacgug     1500

aacaagcagg agggcaagag ccucuacgug aagggggagc ccaucaucaa cuucuacgac     1560

ccgcuggucu uccccuccga cgaguucgac gcgagcaucu cccaggugaa cgagaagauc     1620

aaccagagcc uggccuucau ccgcaagucc gacgagcugc uccacaacgu gaacgccggc     1680

aagagcacga ccaacaucau gaucaccacc aucaucaucg ucaucaucgu gauccugcug     1740

ucccucaucg ccguggggcu gcugcucuac ugcaaggccc ggugaggacu agugaaggga     1800

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa     1860

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu     1920

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua     1980

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua     2040

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     2100

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca     2220

uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc caccagaauu     2280


<210>  257
<211>  1973
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  257
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuaguggaa gcauuuuccu ggcugauuaa aagaaauuac     1740

ucagcuaugg ucaucuguuc cuguuagaag gcuaugcagc auauuauaua cuaugcgcau     1800

guuaugaaau gcauaauaaa aaauuuuaaa aaaucuaaaa gaucuaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc     1920

cccccccccc cccccccccc cccccaaagg cucuuuucag agccaccaga auu            1973


<210>  258
<211>  1914
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  258
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuaguggaa gcauuuuccu ggcugauuaa aagaaauuac     1740

ucagcuaugg ucaucuguuc cuguuagaag gcuaugcagc auauuauaua cuaugcgcau     1800

guuaugaaau gcauaauaaa aaauuuuaaa aaaucuaaaa gaucuaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           1914


<210>  259
<211>  980
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  259
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu aguggaagca      720

uuuuccuggc ugauuaaaag aaauuacuca gcuaugguca ucuguuccug uuagaaggcu      780

augcagcaua uuauauacua ugcgcauguu augaaaugca uaauaaaaaa uuuuaaaaaa      840

ucuaaaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc      960

uuuucagagc caccagaauu                                                  980


<210>  260
<211>  921
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  260
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu aguggaagca      720

uuuuccuggc ugauuaaaag aaauuacuca gcuaugguca ucuguuccug uuagaaggcu      780

augcagcaua uuauauacua ugcgcauguu augaaaugca uaauaaaaaa uuuuaaaaaa      840

ucuaaaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaaaaaaa aaaaaagaau u                                                921


<210>  261
<211>  2051
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  261
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uaguggaagc auuuuccugg     1800

cugauuaaaa gaaauuacuc agcuaugguc aucuguuccu guuagaaggc uaugcagcau     1860

auuauauacu augcgcaugu uaugaaaugc auaauaaaaa auuuuaaaaa aucuaaaaga     1920

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag     2040

ccaccagaau u                                                          2051


<210>  262
<211>  1992
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  262
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uaguggaagc auuuuccugg     1800

cugauuaaaa gaaauuacuc agcuaugguc aucuguuccu guuagaaggc uaugcagcau     1860

auuauauacu augcgcaugu uaugaaaugc auaauaaaaa auuuuaaaaa aucuaaaaga     1920

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaaaagaa uu                                                         1992


<210>  263
<211>  2060
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  263
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagc ugcucauccu gaaggccaac gccaucacca ccauccugac ggcggugacc      180

uucugcuucg ccagcggcca gaacaucacc gaggaguucu accaguccac gugcagcgcc      240

gucuccaagg gguaccucag cgcccugcgg accggcuggu acaccuccgu gaucaccauc      300

gagcugagca acaucaagga gaacaagugc aacgggaccg acgccaaggu gaagcucauc      360

aagcaggagc uggacaagua caagaacgcg gucacggagc ugcagcuccu gaugcagucc      420

accccggcaa ccaacaaccg cgcccggcgc gagcugcccc gguucaugaa cuacacccuc      480

aacaacgcca agaagacgaa cgugacccug agcaagaagc gcaagcggcg cuuccugggc      540

uuccuccugg gggugggcuc cgccaucgcg agcggcgucg ccgugugcaa ggugcugcac      600

cucgaggggg aggucaacaa gaucaagagc gcccugcugu ccaccaacaa ggccguggug      660

agccucucca acggcgucag cgugcugacc uucaaggugc uggaccucaa gaacuacauc      720

gacaagcagc ugcugcccau ccugaacaag cagagcugcu ccaucagcaa caucgagacg      780

gugaucgagu uccagcagaa gaacaaccgg cuccuggaga ucacccgcga guucagcgug      840

aacgccgggg ucaccacccc cguguccacg uacaugcuga ccaacagcga gcuccugucc      900

cugaucaacg acaugccgau caccaacgac cagaagaagc ucaugagcaa caacgugcag      960

aucguccggc agcaguccua cagcaucaug ugcaucauca aggaggaggu gcuggcguac     1020

gugguccagc ugccccucua cggcgugauc gacacccccu gcuggaagcu gcacacgagc     1080

ccccugugca ccaccaacac caaggagggg uccaacaucu gccucacgcg caccgaccgg     1140

ggcugguacu gcgacaacgc cggcagcguc uccuucuucc cgcaggccga gaccugcaag     1200

gugcagagca accgcguguu cugcgacacc augaacuccc ugacgcugcc cagcgaggua     1260

aaccucugca acgucgacau cuucaacccc aaguacgacu gcaagaucau gaccuccaag     1320

accgacguga gcuccagcgu gaucaccucc cugggggcca ucgucagcug cuacggcaag     1380

acgaagugca ccgccagcaa caagaaccgg gggaucauca agaccuucuc caacggcugc     1440

gacuacguga gcaacaaggg cguggacacc guguccgucg ggaacacccu guacuacgug     1500

aacaagcagg agggcaagag ccucuacgug aagggggagc ccaucaucaa cuucuacgac     1560

ccgcuggucu uccccuccga cgaguucgac gcgagcaucu cccaggugaa cgagaagauc     1620

aaccagagcc uggccuucau ccgcaagucc gacgagcugc uccacaacgu gaacgccggc     1680

aagagcacga ccaacaucau gaucaccacc aucaucaucg ucaucaucgu gauccugcug     1740

ucccucaucg ccguggggcu gcugcucuac ugcaaggccc ggugaggacu aguggaagca     1800

uuuuccuggc ugauuaaaag aaauuacuca gcuaugguca ucuguuccug uuagaaggcu     1860

augcagcaua uuauauacua ugcgcauguu augaaaugca uaauaaaaaa uuuuaaaaaa     1920

ucuaaaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc     2040

uuuucagagc caccagaauu                                                 2060


<210>  264
<211>  1910
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  264
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuagugucc accugucccu ccugggcugc uggauugucu     1740

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc     1860

cccccccccc cccccccccc ccaaaggcuc uuuucagagc caccagaauu                1910


<210>  265
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  265
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggugc cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc      180

aaguucccca ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac      240

caccuguccu gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg      300

uucuccuaca uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg      360

ugcaccggcg uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc      420

accuucaagc ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg      480

aagauggccg gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac      540

uggcugcgga ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg      600

gaccucgacc ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc      660

ggcguggccg ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc      720

gagaacccgc gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc      780

uccaagggca gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag      840

ggggccugca agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg      900

guggcgaugc agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug      960

cacgacuucc ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc     1020

gaggagugcc uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc     1080

cugagccacc uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag     1140

acccugaugg aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg     1200

agcaaggggu gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc     1260

aacggcauca uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug     1320

cuccagcagc acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg     1380

gaccccucca ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc     1440

cccgacgugc acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac     1500

gugcugcucu ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc     1560

uggcgccggg ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag     1620

gucuccguga ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc     1680

ggcgagaccg ggcugugagg acuagugucc accugucccu ccugggcugc uggauugucu     1740

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              1851


<210>  266
<211>  917
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  266
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu aguguccacc      720

ugucccuccu gggcugcugg auugucucgu uuuccugcca aauaaacagg aucagcgcuu      780

uacagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu      900

ucagagccac cagaauu                                                     917


<210>  267
<211>  858
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  267
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accaugggcg ugcacgagug ccccgccugg cuguggcucc ugcugagccu ccugucccug      180

ccgcucgggc ugcccguccu gggcgccccc ccgcggcuca ucugcgacag ccgcgugcug      240

gagcgguacc ugcucgaggc gaaggaggcc gagaacauca ccaccgggug cgccgagcac      300

ugcucccuga acgagaacau cacggugccg gacaccaagg ucaacuucua cgccuggaag      360

cgcauggagg ugggccagca ggccguggag gucuggcagg ggcuggcgcu ccugagcgag      420

gccgugcugc ggggccaggc ccuccuggug aacuccagcc agcccuggga gccccugcag      480

cuccacgucg acaaggccgu guccggccug cgcagccuga ccacccuccu gcgggcgcug      540

ggggcccaga aggaggccau cucccccccg gacgccgcca gcgcggcccc gcuccgcacg      600

aucaccgccg acaccuuccg gaagcuguuc cgcguguacu ccaacuuccu gcggggcaag      660

cucaagcugu acaccgggga ggccugccgc acgggcgacc ggugaggacu aguguccacc      720

ugucccuccu gggcugcugg auugucucgu uuuccugcca aauaaacagg aucagcgcuu      780

uacagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaagaauu                                                    858


<210>  268
<211>  1988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  268
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uaguguccac cugucccucc     1800

ugggcugcug gauugucucg uuuuccugcc aaauaaacag gaucagcgcu uuacagaucu     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca     1980

ccagaauu                                                              1988


<210>  269
<211>  1929
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  269
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagg acgccaagaa caucaagaag ggcccggcgc ccuucuaccc gcuggaggac      180

gggaccgccg gcgagcagcu ccacaaggcc augaagcggu acgcccuggu gccgggcacg      240

aucgccuuca ccgacgccca caucgagguc gacaucaccu acgcggagua cuucgagaug      300

agcgugcgcc uggccgaggc caugaagcgg uacggccuga acaccaacca ccggaucgug      360

gugugcucgg agaacagccu gcaguucuuc augccggugc ugggcgcccu cuucaucggc      420

guggccgucg ccccggcgaa cgacaucuac aacgagcggg agcugcugaa cagcaugggg      480

aucagccagc cgaccguggu guucgugagc aagaagggcc ugcagaagau ccugaacgug      540

cagaagaagc ugcccaucau ccagaagauc aucaucaugg acagcaagac cgacuaccag      600

ggcuuccagu cgauguacac guucgugacc agccaccucc cgccgggcuu caacgaguac      660

gacuucgucc cggagagcuu cgaccgggac aagaccaucg cccugaucau gaacagcagc      720

ggcagcaccg gccugccgaa ggggguggcc cugccgcacc ggaccgccug cgugcgcuuc      780

ucgcacgccc gggaccccau cuucggcaac cagaucaucc cggacaccgc cauccugagc      840

guggugccgu uccaccacgg cuucggcaug uucacgaccc ugggcuaccu caucugcggc      900

uuccgggugg uccugaugua ccgguucgag gaggagcugu uccugcggag ccugcaggac      960

uacaagaucc agagcgcgcu gcucgugccg acccuguuca gcuucuucgc caagagcacc     1020

cugaucgaca aguacgaccu gucgaaccug cacgagaucg ccagcggggg cgccccgcug     1080

agcaaggagg ugggcgaggc cguggccaag cgguuccacc ucccgggcau ccgccagggc     1140

uacggccuga ccgagaccac gagcgcgauc cugaucaccc ccgaggggga cgacaagccg     1200

ggcgccgugg gcaagguggu cccguucuuc gaggccaagg ugguggaccu ggacaccggc     1260

aagacccugg gcgugaacca gcggggcgag cugugcgugc gggggccgau gaucaugagc     1320

ggcuacguga acaacccgga ggccaccaac gcccucaucg acaaggacgg cuggcugcac     1380

agcggcgaca ucgccuacug ggacgaggac gagcacuucu ucaucgucga ccggcugaag     1440

ucgcugauca aguacaaggg cuaccaggug gcgccggccg agcuggagag cauccugcuc     1500

cagcacccca acaucuucga cgccggcgug gccgggcugc cggacgacga cgccggcgag     1560

cugccggccg cggugguggu gcuggagcac ggcaagacca ugacggagaa ggagaucguc     1620

gacuacgugg ccagccaggu gaccaccgcc aagaagcugc ggggcggcgu gguguucgug     1680

gacgaggucc cgaagggccu gaccgggaag cucgacgccc ggaagauccg cgagauccug     1740

aucaaggcca agaagggcgg caagaucgcc gugugaggac uaguguccac cugucccucc     1800

ugggcugcug gauugucucg uuuuccugcc aaauaaacag gaucagcgcu uuacagaucu     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaagaauu                                                             1929


<210>  270
<211>  1997
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  270
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggagc ugcucauccu gaaggccaac gccaucacca ccauccugac ggcggugacc      180

uucugcuucg ccagcggcca gaacaucacc gaggaguucu accaguccac gugcagcgcc      240

gucuccaagg gguaccucag cgcccugcgg accggcuggu acaccuccgu gaucaccauc      300

gagcugagca acaucaagga gaacaagugc aacgggaccg acgccaaggu gaagcucauc      360

aagcaggagc uggacaagua caagaacgcg gucacggagc ugcagcuccu gaugcagucc      420

accccggcaa ccaacaaccg cgcccggcgc gagcugcccc gguucaugaa cuacacccuc      480

aacaacgcca agaagacgaa cgugacccug agcaagaagc gcaagcggcg cuuccugggc      540

uuccuccugg gggugggcuc cgccaucgcg agcggcgucg ccgugugcaa ggugcugcac      600

cucgaggggg aggucaacaa gaucaagagc gcccugcugu ccaccaacaa ggccguggug      660

agccucucca acggcgucag cgugcugacc uucaaggugc uggaccucaa gaacuacauc      720

gacaagcagc ugcugcccau ccugaacaag cagagcugcu ccaucagcaa caucgagacg      780

gugaucgagu uccagcagaa gaacaaccgg cuccuggaga ucacccgcga guucagcgug      840

aacgccgggg ucaccacccc cguguccacg uacaugcuga ccaacagcga gcuccugucc      900

cugaucaacg acaugccgau caccaacgac cagaagaagc ucaugagcaa caacgugcag      960

aucguccggc agcaguccua cagcaucaug ugcaucauca aggaggaggu gcuggcguac     1020

gugguccagc ugccccucua cggcgugauc gacacccccu gcuggaagcu gcacacgagc     1080

ccccugugca ccaccaacac caaggagggg uccaacaucu gccucacgcg caccgaccgg     1140

ggcugguacu gcgacaacgc cggcagcguc uccuucuucc cgcaggccga gaccugcaag     1200

gugcagagca accgcguguu cugcgacacc augaacuccc ugacgcugcc cagcgaggua     1260

aaccucugca acgucgacau cuucaacccc aaguacgacu gcaagaucau gaccuccaag     1320

accgacguga gcuccagcgu gaucaccucc cugggggcca ucgucagcug cuacggcaag     1380

acgaagugca ccgccagcaa caagaaccgg gggaucauca agaccuucuc caacggcugc     1440

gacuacguga gcaacaaggg cguggacacc guguccgucg ggaacacccu guacuacgug     1500

aacaagcagg agggcaagag ccucuacgug aagggggagc ccaucaucaa cuucuacgac     1560

ccgcuggucu uccccuccga cgaguucgac gcgagcaucu cccaggugaa cgagaagauc     1620

aaccagagcc uggccuucau ccgcaagucc gacgagcugc uccacaacgu gaacgccggc     1680

aagagcacga ccaacaucau gaucaccacc aucaucaucg ucaucaucgu gauccugcug     1740

ucccucaucg ccguggggcu gcugcucuac ugcaaggccc ggugaggacu aguguccacc     1800

ugucccuccu gggcugcugg auugucucgu uuuccugcca aauaaacagg aucagcgcuu     1860

uacagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu     1980

ucagagccac cagaauu                                                    1997


<210>  271
<211>  1873
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  271
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     1740

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1860

agccaccaga auu                                                        1873


<210>  272
<211>  1814
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  272
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     1740

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaag aauu                                                       1814


<210>  273
<211>  880
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  273
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu      720

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc      840

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                            880


<210>  274
<211>  821
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  274
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu      720

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                          821


<210>  275
<211>  1951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  275
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau     1800

aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1920

cccaaaggcu cuuuucagag ccaccagaau u                                    1951


<210>  276
<211>  1892
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  276
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau     1800

aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   1892


<210>  277
<211>  1960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  277
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggagcugcu cauccugaag      120

gccaacgcca ucaccaccau ccugacggcg gugaccuucu gcuucgccag cggccagaac      180

aucaccgagg aguucuacca guccacgugc agcgccgucu ccaaggggua ccucagcgcc      240

cugcggaccg gcugguacac cuccgugauc accaucgagc ugagcaacau caaggagaac      300

aagugcaacg ggaccgacgc caaggugaag cucaucaagc aggagcugga caaguacaag      360

aacgcgguca cggagcugca gcuccugaug caguccaccc cggcaaccaa caaccgcgcc      420

cggcgcgagc ugccccgguu caugaacuac acccucaaca acgccaagaa gacgaacgug      480

acccugagca agaagcgcaa gcggcgcuuc cugggcuucc uccugggggu gggcuccgcc      540

aucgcgagcg gcgucgccgu gugcaaggug cugcaccucg agggggaggu caacaagauc      600

aagagcgccc ugcuguccac caacaaggcc guggugagcc ucuccaacgg cgucagcgug      660

cugaccuuca aggugcugga ccucaagaac uacaucgaca agcagcugcu gcccauccug      720

aacaagcaga gcugcuccau cagcaacauc gagacgguga ucgaguucca gcagaagaac      780

aaccggcucc uggagaucac ccgcgaguuc agcgugaacg ccggggucac cacccccgug      840

uccacguaca ugcugaccaa cagcgagcuc cugucccuga ucaacgacau gccgaucacc      900

aacgaccaga agaagcucau gagcaacaac gugcagaucg uccggcagca guccuacagc      960

aucaugugca ucaucaagga ggaggugcug gcguacgugg uccagcugcc ccucuacggc     1020

gugaucgaca cccccugcug gaagcugcac acgagccccc ugugcaccac caacaccaag     1080

gaggggucca acaucugccu cacgcgcacc gaccggggcu gguacugcga caacgccggc     1140

agcgucuccu ucuucccgca ggccgagacc ugcaaggugc agagcaaccg cguguucugc     1200

gacaccauga acucccugac gcugcccagc gagguaaacc ucugcaacgu cgacaucuuc     1260

aaccccaagu acgacugcaa gaucaugacc uccaagaccg acgugagcuc cagcgugauc     1320

accucccugg gggccaucgu cagcugcuac ggcaagacga agugcaccgc cagcaacaag     1380

aaccggggga ucaucaagac cuucuccaac ggcugcgacu acgugagcaa caagggcgug     1440

gacaccgugu ccgucgggaa cacccuguac uacgugaaca agcaggaggg caagagccuc     1500

uacgugaagg gggagcccau caucaacuuc uacgacccgc uggucuuccc cuccgacgag     1560

uucgacgcga gcaucuccca ggugaacgag aagaucaacc agagccuggc cuucauccgc     1620

aaguccgacg agcugcucca caacgugaac gccggcaaga gcacgaccaa caucaugauc     1680

accaccauca ucaucgucau caucgugauc cugcuguccc ucaucgccgu ggggcugcug     1740

cucuacugca aggcccggug aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     1800

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     1920

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           1960


<210>  278
<211>  1937
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  278
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

guaauaagga aacuguauga augucugugg gcaggaagug aagagauccu ucuguaaagg     1740

uuuuuggaau uaugucugcu gaauaauaaa cuuuuuugaa auaauaaauc ugguagaaaa     1800

augagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu     1920

ucagagccac cagaauu                                                    1937


<210>  279
<211>  1878
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  279
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

guaauaagga aacuguauga augucugugg gcaggaagug aagagauccu ucuguaaagg     1740

uuuuuggaau uaugucugcu gaauaauaaa cuuuuuugaa auaauaaauc ugguagaaaa     1800

augagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaagaauu                                                   1878


<210>  280
<211>  944
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  280
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagua auaaggaaac uguaugaaug ucugugggca      720

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu      780

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc      900

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                       944


<210>  281
<211>  885
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  281
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagua auaaggaaac uguaugaaug ucugugggca      720

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu      780

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                      885


<210>  282
<211>  2015
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  282
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     1800

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     1860

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc     1980

cccccccaaa ggcucuuuuc agagccacca gaauu                                2015


<210>  283
<211>  1956
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  283
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     1800

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     1860

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               1956


<210>  284
<211>  2024
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  284
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggagcugcu cauccugaag      120

gccaacgcca ucaccaccau ccugacggcg gugaccuucu gcuucgccag cggccagaac      180

aucaccgagg aguucuacca guccacgugc agcgccgucu ccaaggggua ccucagcgcc      240

cugcggaccg gcugguacac cuccgugauc accaucgagc ugagcaacau caaggagaac      300

aagugcaacg ggaccgacgc caaggugaag cucaucaagc aggagcugga caaguacaag      360

aacgcgguca cggagcugca gcuccugaug caguccaccc cggcaaccaa caaccgcgcc      420

cggcgcgagc ugccccgguu caugaacuac acccucaaca acgccaagaa gacgaacgug      480

acccugagca agaagcgcaa gcggcgcuuc cugggcuucc uccugggggu gggcuccgcc      540

aucgcgagcg gcgucgccgu gugcaaggug cugcaccucg agggggaggu caacaagauc      600

aagagcgccc ugcuguccac caacaaggcc guggugagcc ucuccaacgg cgucagcgug      660

cugaccuuca aggugcugga ccucaagaac uacaucgaca agcagcugcu gcccauccug      720

aacaagcaga gcugcuccau cagcaacauc gagacgguga ucgaguucca gcagaagaac      780

aaccggcucc uggagaucac ccgcgaguuc agcgugaacg ccggggucac cacccccgug      840

uccacguaca ugcugaccaa cagcgagcuc cugucccuga ucaacgacau gccgaucacc      900

aacgaccaga agaagcucau gagcaacaac gugcagaucg uccggcagca guccuacagc      960

aucaugugca ucaucaagga ggaggugcug gcguacgugg uccagcugcc ccucuacggc     1020

gugaucgaca cccccugcug gaagcugcac acgagccccc ugugcaccac caacaccaag     1080

gaggggucca acaucugccu cacgcgcacc gaccggggcu gguacugcga caacgccggc     1140

agcgucuccu ucuucccgca ggccgagacc ugcaaggugc agagcaaccg cguguucugc     1200

gacaccauga acucccugac gcugcccagc gagguaaacc ucugcaacgu cgacaucuuc     1260

aaccccaagu acgacugcaa gaucaugacc uccaagaccg acgugagcuc cagcgugauc     1320

accucccugg gggccaucgu cagcugcuac ggcaagacga agugcaccgc cagcaacaag     1380

aaccggggga ucaucaagac cuucuccaac ggcugcgacu acgugagcaa caagggcgug     1440

gacaccgugu ccgucgggaa cacccuguac uacgugaaca agcaggaggg caagagccuc     1500

uacgugaagg gggagcccau caucaacuuc uacgacccgc uggucuuccc cuccgacgag     1560

uucgacgcga gcaucuccca ggugaacgag aagaucaacc agagccuggc cuucauccgc     1620

aaguccgacg agcugcucca caacgugaac gccggcaaga gcacgaccaa caucaugauc     1680

accaccauca ucaucgucau caucgugauc cugcuguccc ucaucgccgu ggggcugcug     1740

cucuacugca aggcccggug aggacuagua auaaggaaac uguaugaaug ucugugggca     1800

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     1860

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc     1980

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                      2024


<210>  285
<211>  1953
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  285
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

guacuggcug caucucccuu uccucugucc uccauccuuc ucccaggaug gugaaggggg     1740

accugguacc cagugauccc caccccagga uccuaaauca ugacuuaccu gcuaauaaaa     1800

acucauugga aaagugagaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc cccccccccc     1920

cccccaaagg cucuuuucag agccaccaga auu                                  1953


<210>  286
<211>  1894
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  286
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

guacuggcug caucucccuu uccucugucc uccauccuuc ucccaggaug gugaaggggg     1740

accugguacc cagugauccc caccccagga uccuaaauca ugacuuaccu gcuaauaaaa     1800

acucauugga aaagugagaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                                 1894


<210>  287
<211>  960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  287
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagua cuggcugcau cucccuuucc ucuguccucc      720

auccuucucc caggauggug aagggggacc ugguacccag ugauccccac cccaggaucc      780

uaaaucauga cuuaccugcu aauaaaaacu cauuggaaaa gugagaagau cuaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca      900

uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc caccagaauu      960


<210>  288
<211>  901
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  288
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagua cuggcugcau cucccuuucc ucuguccucc      720

auccuucucc caggauggug aagggggacc ugguacccag ugauccccac cccaggaucc      780

uaaaucauga cuuaccugcu aauaaaaacu cauuggaaaa gugagaagau cuaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau      900

u                                                                      901


<210>  289
<211>  2031
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  289
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu acuggcugca ucucccuuuc cucuguccuc cauccuucuc     1800

ccaggauggu gaagggggac cugguaccca gugaucccca ccccaggauc cuaaaucaug     1860

acuuaccugc uaauaaaaac ucauuggaaa agugagaaga ucuaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc     1980

cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau u              2031


<210>  290
<211>  1972
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  290
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu acuggcugca ucucccuuuc cucuguccuc cauccuucuc     1800

ccaggauggu gaagggggac cugguaccca gugaucccca ccccaggauc cuaaaucaug     1860

acuuaccugc uaauaaaaac ucauuggaaa agugagaaga ucuaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             1972


<210>  291
<211>  2040
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  291
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggagcugcu cauccugaag      120

gccaacgcca ucaccaccau ccugacggcg gugaccuucu gcuucgccag cggccagaac      180

aucaccgagg aguucuacca guccacgugc agcgccgucu ccaaggggua ccucagcgcc      240

cugcggaccg gcugguacac cuccgugauc accaucgagc ugagcaacau caaggagaac      300

aagugcaacg ggaccgacgc caaggugaag cucaucaagc aggagcugga caaguacaag      360

aacgcgguca cggagcugca gcuccugaug caguccaccc cggcaaccaa caaccgcgcc      420

cggcgcgagc ugccccgguu caugaacuac acccucaaca acgccaagaa gacgaacgug      480

acccugagca agaagcgcaa gcggcgcuuc cugggcuucc uccugggggu gggcuccgcc      540

aucgcgagcg gcgucgccgu gugcaaggug cugcaccucg agggggaggu caacaagauc      600

aagagcgccc ugcuguccac caacaaggcc guggugagcc ucuccaacgg cgucagcgug      660

cugaccuuca aggugcugga ccucaagaac uacaucgaca agcagcugcu gcccauccug      720

aacaagcaga gcugcuccau cagcaacauc gagacgguga ucgaguucca gcagaagaac      780

aaccggcucc uggagaucac ccgcgaguuc agcgugaacg ccggggucac cacccccgug      840

uccacguaca ugcugaccaa cagcgagcuc cugucccuga ucaacgacau gccgaucacc      900

aacgaccaga agaagcucau gagcaacaac gugcagaucg uccggcagca guccuacagc      960

aucaugugca ucaucaagga ggaggugcug gcguacgugg uccagcugcc ccucuacggc     1020

gugaucgaca cccccugcug gaagcugcac acgagccccc ugugcaccac caacaccaag     1080

gaggggucca acaucugccu cacgcgcacc gaccggggcu gguacugcga caacgccggc     1140

agcgucuccu ucuucccgca ggccgagacc ugcaaggugc agagcaaccg cguguucugc     1200

gacaccauga acucccugac gcugcccagc gagguaaacc ucugcaacgu cgacaucuuc     1260

aaccccaagu acgacugcaa gaucaugacc uccaagaccg acgugagcuc cagcgugauc     1320

accucccugg gggccaucgu cagcugcuac ggcaagacga agugcaccgc cagcaacaag     1380

aaccggggga ucaucaagac cuucuccaac ggcugcgacu acgugagcaa caagggcgug     1440

gacaccgugu ccgucgggaa cacccuguac uacgugaaca agcaggaggg caagagccuc     1500

uacgugaagg gggagcccau caucaacuuc uacgacccgc uggucuuccc cuccgacgag     1560

uucgacgcga gcaucuccca ggugaacgag aagaucaacc agagccuggc cuucauccgc     1620

aaguccgacg agcugcucca caacgugaac gccggcaaga gcacgaccaa caucaugauc     1680

accaccauca ucaucgucau caucgugauc cugcuguccc ucaucgccgu ggggcugcug     1740

cucuacugca aggcccggug aggacuagua cuggcugcau cucccuuucc ucuguccucc     1800

auccuucucc caggauggug aagggggacc ugguacccag ugauccccac cccaggaucc     1860

uaaaucauga cuuaccugcu aauaaaaacu cauuggaaaa gugagaagau cuaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca     1980

uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc caccagaauu     2040


<210>  292
<211>  2169
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  292
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

gugaagggaa cacccaaauu uaauucagcc uuaagcacaa uuaauuaaga gugaaacgua     1740

auuguacaag caguugguca cccaccauag ggcaugauca acaccgcaac cuuuccuuuu     1800

ucccccagug auucugaaaa accccucuuc ccuucagcuu gcuuagaugu uccaaauuua     1860

guaagcuuaa ggcggccuac agaagaaaaa gaaaaaaaag gccacaaaag uucccucuca     1920

cuuucaguaa auaaaauaaa agcagcaaca gaaauaaaga aauaaaugaa auucaaaaug     1980

aaauaaauau uguguugugc agcauuaaaa aaucaauaaa aauuaaaaau gagcaagauc     2040

uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu uuucagagcc     2160

accagaauu                                                             2169


<210>  293
<211>  2110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  293
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

gugaagggaa cacccaaauu uaauucagcc uuaagcacaa uuaauuaaga gugaaacgua     1740

auuguacaag caguugguca cccaccauag ggcaugauca acaccgcaac cuuuccuuuu     1800

ucccccagug auucugaaaa accccucuuc ccuucagcuu gcuuagaugu uccaaauuua     1860

guaagcuuaa ggcggccuac agaagaaaaa gaaaaaaaag gccacaaaag uucccucuca     1920

cuuucaguaa auaaaauaaa agcagcaaca gaaauaaaga aauaaaugaa auucaaaaug     1980

aaauaaauau uguguugugc agcauuaaaa aaucaauaaa aauuaaaaau gagcaagauc     2040

uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaagaauu                                                            2110


<210>  294
<211>  1176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  294
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagug aagggaacac ccaaauuuaa uucagccuua      720

agcacaauua auuaagagug aaacguaauu guacaagcag uuggucaccc accauagggc      780

augaucaaca ccgcaaccuu uccuuuuucc cccagugauu cugaaaaacc ccucuucccu      840

ucagcuugcu uagauguucc aaauuuagua agcuuaaggc ggccuacaga agaaaaagaa      900

aaaaaaggcc acaaaaguuc ccucucacuu ucaguaaaua aaauaaaagc agcaacagaa      960

auaaagaaau aaaugaaauu caaaaugaaa uaaauauugu guugugcagc auuaaaaaau     1020

caauaaaaau uaaaaaugag caagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc cccccccccc     1140

ccccccccaa aggcucuuuu cagagccacc agaauu                               1176


<210>  295
<211>  1117
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  295
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagug aagggaacac ccaaauuuaa uucagccuua      720

agcacaauua auuaagagug aaacguaauu guacaagcag uuggucaccc accauagggc      780

augaucaaca ccgcaaccuu uccuuuuucc cccagugauu cugaaaaacc ccucuucccu      840

ucagcuugcu uagauguucc aaauuuagua agcuuaaggc ggccuacaga agaaaaagaa      900

aaaaaaggcc acaaaaguuc ccucucacuu ucaguaaaua aaauaaaagc agcaacagaa      960

auaaagaaau aaaugaaauu caaaaugaaa uaaauauugu guugugcagc auuaaaaaau     1020

caauaaaaau uaaaaaugag caagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                              1117


<210>  296
<211>  2247
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  296
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu     1800

aauuaagagu gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac     1860

accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc     1920

uuagauguuc caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc     1980

cacaaaaguu cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa     2040

uaaaugaaau ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa     2100

uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc ccccccccca     2220

aaggcucuuu ucagagccac cagaauu                                         2247


<210>  297
<211>  2188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  297
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu     1800

aauuaagagu gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac     1860

accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc     1920

uuagauguuc caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc     1980

cacaaaaguu cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa     2040

uaaaugaaau ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa     2100

uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        2188


<210>  298
<211>  2256
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  298
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggagcugcu cauccugaag      120

gccaacgcca ucaccaccau ccugacggcg gugaccuucu gcuucgccag cggccagaac      180

aucaccgagg aguucuacca guccacgugc agcgccgucu ccaaggggua ccucagcgcc      240

cugcggaccg gcugguacac cuccgugauc accaucgagc ugagcaacau caaggagaac      300

aagugcaacg ggaccgacgc caaggugaag cucaucaagc aggagcugga caaguacaag      360

aacgcgguca cggagcugca gcuccugaug caguccaccc cggcaaccaa caaccgcgcc      420

cggcgcgagc ugccccgguu caugaacuac acccucaaca acgccaagaa gacgaacgug      480

acccugagca agaagcgcaa gcggcgcuuc cugggcuucc uccugggggu gggcuccgcc      540

aucgcgagcg gcgucgccgu gugcaaggug cugcaccucg agggggaggu caacaagauc      600

aagagcgccc ugcuguccac caacaaggcc guggugagcc ucuccaacgg cgucagcgug      660

cugaccuuca aggugcugga ccucaagaac uacaucgaca agcagcugcu gcccauccug      720

aacaagcaga gcugcuccau cagcaacauc gagacgguga ucgaguucca gcagaagaac      780

aaccggcucc uggagaucac ccgcgaguuc agcgugaacg ccggggucac cacccccgug      840

uccacguaca ugcugaccaa cagcgagcuc cugucccuga ucaacgacau gccgaucacc      900

aacgaccaga agaagcucau gagcaacaac gugcagaucg uccggcagca guccuacagc      960

aucaugugca ucaucaagga ggaggugcug gcguacgugg uccagcugcc ccucuacggc     1020

gugaucgaca cccccugcug gaagcugcac acgagccccc ugugcaccac caacaccaag     1080

gaggggucca acaucugccu cacgcgcacc gaccggggcu gguacugcga caacgccggc     1140

agcgucuccu ucuucccgca ggccgagacc ugcaaggugc agagcaaccg cguguucugc     1200

gacaccauga acucccugac gcugcccagc gagguaaacc ucugcaacgu cgacaucuuc     1260

aaccccaagu acgacugcaa gaucaugacc uccaagaccg acgugagcuc cagcgugauc     1320

accucccugg gggccaucgu cagcugcuac ggcaagacga agugcaccgc cagcaacaag     1380

aaccggggga ucaucaagac cuucuccaac ggcugcgacu acgugagcaa caagggcgug     1440

gacaccgugu ccgucgggaa cacccuguac uacgugaaca agcaggaggg caagagccuc     1500

uacgugaagg gggagcccau caucaacuuc uacgacccgc uggucuuccc cuccgacgag     1560

uucgacgcga gcaucuccca ggugaacgag aagaucaacc agagccuggc cuucauccgc     1620

aaguccgacg agcugcucca caacgugaac gccggcaaga gcacgaccaa caucaugauc     1680

accaccauca ucaucgucau caucgugauc cugcuguccc ucaucgccgu ggggcugcug     1740

cucuacugca aggcccggug aggacuagug aagggaacac ccaaauuuaa uucagccuua     1800

agcacaauua auuaagagug aaacguaauu guacaagcag uuggucaccc accauagggc     1860

augaucaaca ccgcaaccuu uccuuuuucc cccagugauu cugaaaaacc ccucuucccu     1920

ucagcuugcu uagauguucc aaauuuagua agcuuaaggc ggccuacaga agaaaaagaa     1980

aaaaaaggcc acaaaaguuc ccucucacuu ucaguaaaua aaauaaaagc agcaacagaa     2040

auaaagaaau aaaugaaauu caaaaugaaa uaaauauugu guugugcagc auuaaaaaau     2100

caauaaaaau uaaaaaugag caagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc cccccccccc     2220

ccccccccaa aggcucuuuu cagagccacc agaauu                               2256


<210>  299
<211>  1949
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  299
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

guggaagcau uuuccuggcu gauuaaaaga aauuacucag cuauggucau cuguuccugu     1740

uagaaggcua ugcagcauau uauauacuau gcgcauguua ugaaaugcau aauaaaaaau     1800

uuuaaaaaau cuaaaagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc     1920

caaaggcucu uuucagagcc accagaauu                                       1949


<210>  300
<211>  1890
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  300
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

guggaagcau uuuccuggcu gauuaaaaga aauuacucag cuauggucau cuguuccugu     1740

uagaaggcua ugcagcauau uauauacuau gcgcauguua ugaaaugcau aauaaaaaau     1800

uuuaaaaaau cuaaaagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      1890


<210>  301
<211>  956
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  301
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagug gaagcauuuu ccuggcugau uaaaagaaau      720

uacucagcua uggucaucug uuccuguuag aaggcuaugc agcauauuau auacuaugcg      780

cauguuauga aaugcauaau aaaaaauuuu aaaaaaucua aaagaucuaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc      900

cccccccccc cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu          956


<210>  302
<211>  897
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  302
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagug gaagcauuuu ccuggcugau uaaaagaaau      720

uacucagcua uggucaucug uuccuguuag aaggcuaugc agcauauuau auacuaugcg      780

cauguuauga aaugcauaau aaaaaauuuu aaaaaaucua aaagaucuaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu         897


<210>  303
<211>  2027
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  303
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu ggaagcauuu uccuggcuga uuaaaagaaa uuacucagcu     1800

auggucaucu guuccuguua gaaggcuaug cagcauauua uauacuaugc gcauguuaug     1860

aaaugcauaa uaaaaaauuu uaaaaaaucu aaaagaucua aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc     1980

cccccccccc ccccccccca aaggcucuuu ucagagccac cagaauu                   2027


<210>  304
<211>  1968
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  304
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu ggaagcauuu uccuggcuga uuaaaagaaa uuacucagcu     1800

auggucaucu guuccuguua gaaggcuaug cagcauauua uauacuaugc gcauguuaug     1860

aaaugcauaa uaaaaaauuu uaaaaaaucu aaaagaucua aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  1968


<210>  305
<211>  2036
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  305
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggagcugcu cauccugaag      120

gccaacgcca ucaccaccau ccugacggcg gugaccuucu gcuucgccag cggccagaac      180

aucaccgagg aguucuacca guccacgugc agcgccgucu ccaaggggua ccucagcgcc      240

cugcggaccg gcugguacac cuccgugauc accaucgagc ugagcaacau caaggagaac      300

aagugcaacg ggaccgacgc caaggugaag cucaucaagc aggagcugga caaguacaag      360

aacgcgguca cggagcugca gcuccugaug caguccaccc cggcaaccaa caaccgcgcc      420

cggcgcgagc ugccccgguu caugaacuac acccucaaca acgccaagaa gacgaacgug      480

acccugagca agaagcgcaa gcggcgcuuc cugggcuucc uccugggggu gggcuccgcc      540

aucgcgagcg gcgucgccgu gugcaaggug cugcaccucg agggggaggu caacaagauc      600

aagagcgccc ugcuguccac caacaaggcc guggugagcc ucuccaacgg cgucagcgug      660

cugaccuuca aggugcugga ccucaagaac uacaucgaca agcagcugcu gcccauccug      720

aacaagcaga gcugcuccau cagcaacauc gagacgguga ucgaguucca gcagaagaac      780

aaccggcucc uggagaucac ccgcgaguuc agcgugaacg ccggggucac cacccccgug      840

uccacguaca ugcugaccaa cagcgagcuc cugucccuga ucaacgacau gccgaucacc      900

aacgaccaga agaagcucau gagcaacaac gugcagaucg uccggcagca guccuacagc      960

aucaugugca ucaucaagga ggaggugcug gcguacgugg uccagcugcc ccucuacggc     1020

gugaucgaca cccccugcug gaagcugcac acgagccccc ugugcaccac caacaccaag     1080

gaggggucca acaucugccu cacgcgcacc gaccggggcu gguacugcga caacgccggc     1140

agcgucuccu ucuucccgca ggccgagacc ugcaaggugc agagcaaccg cguguucugc     1200

gacaccauga acucccugac gcugcccagc gagguaaacc ucugcaacgu cgacaucuuc     1260

aaccccaagu acgacugcaa gaucaugacc uccaagaccg acgugagcuc cagcgugauc     1320

accucccugg gggccaucgu cagcugcuac ggcaagacga agugcaccgc cagcaacaag     1380

aaccggggga ucaucaagac cuucuccaac ggcugcgacu acgugagcaa caagggcgug     1440

gacaccgugu ccgucgggaa cacccuguac uacgugaaca agcaggaggg caagagccuc     1500

uacgugaagg gggagcccau caucaacuuc uacgacccgc uggucuuccc cuccgacgag     1560

uucgacgcga gcaucuccca ggugaacgag aagaucaacc agagccuggc cuucauccgc     1620

aaguccgacg agcugcucca caacgugaac gccggcaaga gcacgaccaa caucaugauc     1680

accaccauca ucaucgucau caucgugauc cugcuguccc ucaucgccgu ggggcugcug     1740

cucuacugca aggcccggug aggacuagug gaagcauuuu ccuggcugau uaaaagaaau     1800

uacucagcua uggucaucug uuccuguuag aaggcuaugc agcauauuau auacuaugcg     1860

cauguuauga aaugcauaau aaaaaauuuu aaaaaaucua aaagaucuaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc     1980

cccccccccc cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu         2036


<210>  306
<211>  1886
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  306
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

guguccaccu gucccuccug ggcugcugga uugucucguu uuccugccaa auaaacagga     1740

ucagcgcuuu acagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa     1860

aggcucuuuu cagagccacc agaauu                                          1886


<210>  307
<211>  1827
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  307
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggugcccca ggcccuccug      120

uucgucccgc ugcugguguu cccccucugc uucggcaagu uccccaucua caccaucccc      180

gacaagcugg ggccguggag ccccaucgac auccaccacc uguccugccc caacaaccuc      240

guggucgagg acgagggcug caccaaccug agcggguucu ccuacaugga gcugaaggug      300

ggcuacauca gcgccaucaa gaugaacggg uucacgugca ccggcguggu caccgaggcg      360

gagaccuaca cgaacuucgu gggcuacgug accaccaccu ucaagcggaa gcacuuccgc      420

cccacgccgg acgccugccg ggccgccuac aacuggaaga uggccgggga cccccgcuac      480

gaggaguccc uccacaaccc cuaccccgac uaccacuggc ugcggaccgu caagaccacc      540

aaggagagcc uggugaucau cuccccgagc guggcggacc ucgaccccua cgaccgcucc      600

cugcacagcc gggucuuccc cggcgggaac ugcuccggcg uggccgugag cuccacguac      660

ugcagcacca accacgacua caccaucugg augcccgaga acccgcgccu ggggaugucc      720

ugcgacaucu ucaccaacag ccggggcaag cgcgccucca agggcagcga gacgugcggg      780

uucgucgacg agcggggccu cuacaagucc cugaaggggg ccugcaagcu gaagcucugc      840

ggcgugcugg gccugcgccu cauggacggg accugggugg cgaugcagac cagcaacgag      900

accaaguggu gcccccccgg ccagcugguc aaccugcacg acuuccggag cgacgagauc      960

gagcaccucg ugguggagga gcuggucaag aagcgcgagg agugccugga cgcccucgag     1020

uccaucauga cgaccaagag cguguccuuc cggcgccuga gccaccuccg gaagcuggug     1080

cccggguucg gcaaggccua caccaucuuc aacaagaccc ugauggaggc cgacgcccac     1140

uacaaguccg uccgcacgug gaacgagauc aucccgagca aggggugccu gcgggugggc     1200

ggccgcugcc acccccacgu caacggggug uucuucaacg gcaucauccu cgggcccgac     1260

ggcaacgugc ugauccccga gaugcagucc agccugcucc agcagcacau ggagcugcug     1320

gucuccagcg ugaucccgcu caugcacccc cuggcggacc ccuccaccgu guucaagaac     1380

ggggacgagg ccgaggacuu cgucgaggug caccuccccg acgugcacga gcggaucagc     1440

ggcgucgacc ugggccugcc gaacuggggg aaguacgugc ugcucuccgc cggcgcccug     1500

accgcccuga ugcugaucau cuuccucaug accugcuggc gccgggugaa ccggagcgag     1560

cccacgcagc acaaccugcg cgggaccggc cgggaggucu ccgugacccc gcagagcggg     1620

aagaucaucu ccagcuggga guccuacaag agcggcggcg agaccgggcu gugaggacua     1680

guguccaccu gucccuccug ggcugcugga uugucucguu uuccugccaa auaaacagga     1740

ucagcgcuuu acagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aagaauu                                         1827


<210>  308
<211>  893
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  308
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagug uccaccuguc ccuccugggc ugcuggauug      720

ucucguuuuc cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc      840

cccccccccc cccccccccc cccccaaagg cucuuuucag agccaccaga auu             893


<210>  309
<211>  834
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  309
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca ugggcgugca cgagugcccc      120

gccuggcugu ggcuccugcu gagccuccug ucccugccgc ucgggcugcc cguccugggc      180

gcccccccgc ggcucaucug cgacagccgc gugcuggagc gguaccugcu cgaggcgaag      240

gaggccgaga acaucaccac cgggugcgcc gagcacugcu cccugaacga gaacaucacg      300

gugccggaca ccaaggucaa cuucuacgcc uggaagcgca uggagguggg ccagcaggcc      360

guggaggucu ggcaggggcu ggcgcuccug agcgaggccg ugcugcgggg ccaggcccuc      420

cuggugaacu ccagccagcc cugggagccc cugcagcucc acgucgacaa ggccgugucc      480

ggccugcgca gccugaccac ccuccugcgg gcgcuggggg cccagaagga ggccaucucc      540

cccccggacg ccgccagcgc ggccccgcuc cgcacgauca ccgccgacac cuuccggaag      600

cuguuccgcg uguacuccaa cuuccugcgg ggcaagcuca agcuguacac cggggaggcc      660

ugccgcacgg gcgaccggug aggacuagug uccaccuguc ccuccugggc ugcuggauug      720

ucucguuuuc cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu            834


<210>  310
<211>  1964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  310
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu     1800

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc     1920

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                      1964


<210>  311
<211>  1905
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  311
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggaggacgc caagaacauc      120

aagaagggcc cggcgcccuu cuacccgcug gaggacggga ccgccggcga gcagcuccac      180

aaggccauga agcgguacgc ccuggugccg ggcacgaucg ccuucaccga cgcccacauc      240

gaggucgaca ucaccuacgc ggaguacuuc gagaugagcg ugcgccuggc cgaggccaug      300

aagcgguacg gccugaacac caaccaccgg aucguggugu gcucggagaa cagccugcag      360

uucuucaugc cggugcuggg cgcccucuuc aucggcgugg ccgucgcccc ggcgaacgac      420

aucuacaacg agcgggagcu gcugaacagc auggggauca gccagccgac cgugguguuc      480

gugagcaaga agggccugca gaagauccug aacgugcaga agaagcugcc caucauccag      540

aagaucauca ucauggacag caagaccgac uaccagggcu uccagucgau guacacguuc      600

gugaccagcc accucccgcc gggcuucaac gaguacgacu ucgucccgga gagcuucgac      660

cgggacaaga ccaucgcccu gaucaugaac agcagcggca gcaccggccu gccgaagggg      720

guggcccugc cgcaccggac cgccugcgug cgcuucucgc acgcccggga ccccaucuuc      780

ggcaaccaga ucaucccgga caccgccauc cugagcgugg ugccguucca ccacggcuuc      840

ggcauguuca cgacccuggg cuaccucauc ugcggcuucc gggugguccu gauguaccgg      900

uucgaggagg agcuguuccu gcggagccug caggacuaca agauccagag cgcgcugcuc      960

gugccgaccc uguucagcuu cuucgccaag agcacccuga ucgacaagua cgaccugucg     1020

aaccugcacg agaucgccag cgggggcgcc ccgcugagca aggagguggg cgaggccgug     1080

gccaagcggu uccaccuccc gggcauccgc cagggcuacg gccugaccga gaccacgagc     1140

gcgauccuga ucacccccga gggggacgac aagccgggcg ccgugggcaa gguggucccg     1200

uucuucgagg ccaagguggu ggaccuggac accggcaaga cccugggcgu gaaccagcgg     1260

ggcgagcugu gcgugcgggg gccgaugauc augagcggcu acgugaacaa cccggaggcc     1320

accaacgccc ucaucgacaa ggacggcugg cugcacagcg gcgacaucgc cuacugggac     1380

gaggacgagc acuucuucau cgucgaccgg cugaagucgc ugaucaagua caagggcuac     1440

cagguggcgc cggccgagcu ggagagcauc cugcuccagc accccaacau cuucgacgcc     1500

ggcguggccg ggcugccgga cgacgacgcc ggcgagcugc cggccgcggu gguggugcug     1560

gagcacggca agaccaugac ggagaaggag aucgucgacu acguggccag ccaggugacc     1620

accgccaaga agcugcgggg cggcguggug uucguggacg aggucccgaa gggccugacc     1680

gggaagcucg acgcccggaa gauccgcgag auccugauca aggccaagaa gggcggcaag     1740

aucgccgugu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu     1800

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     1905


<210>  312
<211>  1973
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  312
gggagacccg ugacccggaa guuguacggc uacgcgacuu ucccucccac aaacccucgc       60

gcccuuccuu uccuacuugg gcccggcaga aagcuuacca uggagcugcu cauccugaag      120

gccaacgcca ucaccaccau ccugacggcg gugaccuucu gcuucgccag cggccagaac      180

aucaccgagg aguucuacca guccacgugc agcgccgucu ccaaggggua ccucagcgcc      240

cugcggaccg gcugguacac cuccgugauc accaucgagc ugagcaacau caaggagaac      300

aagugcaacg ggaccgacgc caaggugaag cucaucaagc aggagcugga caaguacaag      360

aacgcgguca cggagcugca gcuccugaug caguccaccc cggcaaccaa caaccgcgcc      420

cggcgcgagc ugccccgguu caugaacuac acccucaaca acgccaagaa gacgaacgug      480

acccugagca agaagcgcaa gcggcgcuuc cugggcuucc uccugggggu gggcuccgcc      540

aucgcgagcg gcgucgccgu gugcaaggug cugcaccucg agggggaggu caacaagauc      600

aagagcgccc ugcuguccac caacaaggcc guggugagcc ucuccaacgg cgucagcgug      660

cugaccuuca aggugcugga ccucaagaac uacaucgaca agcagcugcu gcccauccug      720

aacaagcaga gcugcuccau cagcaacauc gagacgguga ucgaguucca gcagaagaac      780

aaccggcucc uggagaucac ccgcgaguuc agcgugaacg ccggggucac cacccccgug      840

uccacguaca ugcugaccaa cagcgagcuc cugucccuga ucaacgacau gccgaucacc      900

aacgaccaga agaagcucau gagcaacaac gugcagaucg uccggcagca guccuacagc      960

aucaugugca ucaucaagga ggaggugcug gcguacgugg uccagcugcc ccucuacggc     1020

gugaucgaca cccccugcug gaagcugcac acgagccccc ugugcaccac caacaccaag     1080

gaggggucca acaucugccu cacgcgcacc gaccggggcu gguacugcga caacgccggc     1140

agcgucuccu ucuucccgca ggccgagacc ugcaaggugc agagcaaccg cguguucugc     1200

gacaccauga acucccugac gcugcccagc gagguaaacc ucugcaacgu cgacaucuuc     1260

aaccccaagu acgacugcaa gaucaugacc uccaagaccg acgugagcuc cagcgugauc     1320

accucccugg gggccaucgu cagcugcuac ggcaagacga agugcaccgc cagcaacaag     1380

aaccggggga ucaucaagac cuucuccaac ggcugcgacu acgugagcaa caagggcgug     1440

gacaccgugu ccgucgggaa cacccuguac uacgugaaca agcaggaggg caagagccuc     1500

uacgugaagg gggagcccau caucaacuuc uacgacccgc uggucuuccc cuccgacgag     1560

uucgacgcga gcaucuccca ggugaacgag aagaucaacc agagccuggc cuucauccgc     1620

aaguccgacg agcugcucca caacgugaac gccggcaaga gcacgaccaa caucaugauc     1680

accaccauca ucaucgucau caucgugauc cugcuguccc ucaucgccgu ggggcugcug     1740

cucuacugca aggcccggug aggacuagug uccaccuguc ccuccugggc ugcuggauug     1800

ucucguuuuc cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc     1920

cccccccccc cccccccccc cccccaaagg cucuuuucag agccaccaga auu            1973


<210>  313
<211>  1948
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  313
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     1800

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     1920

aaaggcucuu uucagagcca ccagaauu                                        1948


<210>  314
<211>  1889
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  314
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     1800

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       1889


<210>  315
<211>  955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  315
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uagucccugu ucccagagcc      780

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc      900

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu           955


<210>  316
<211>  896
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  316
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uagucccugu ucccagagcc      780

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu          896


<210>  317
<211>  2026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  317
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuagucccug uucccagagc ccacuuuuuu     1860

uucuuuuuuu gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     1980

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2026


<210>  318
<211>  1967
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  318
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuagucccug uucccagagc ccacuuuuuu     1860

uucuuuuuuu gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   1967


<210>  319
<211>  2035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  319
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

cugcucaucc ugaaggccaa cgccaucacc accauccuga cggcggugac cuucugcuuc      240

gccagcggcc agaacaucac cgaggaguuc uaccagucca cgugcagcgc cgucuccaag      300

ggguaccuca gcgcccugcg gaccggcugg uacaccuccg ugaucaccau cgagcugagc      360

aacaucaagg agaacaagug caacgggacc gacgccaagg ugaagcucau caagcaggag      420

cuggacaagu acaagaacgc ggucacggag cugcagcucc ugaugcaguc caccccggca      480

accaacaacc gcgcccggcg cgagcugccc cgguucauga acuacacccu caacaacgcc      540

aagaagacga acgugacccu gagcaagaag cgcaagcggc gcuuccuggg cuuccuccug      600

ggggugggcu ccgccaucgc gagcggcguc gccgugugca aggugcugca ccucgagggg      660

gaggucaaca agaucaagag cgcccugcug uccaccaaca aggccguggu gagccucucc      720

aacggcguca gcgugcugac cuucaaggug cuggaccuca agaacuacau cgacaagcag      780

cugcugccca uccugaacaa gcagagcugc uccaucagca acaucgagac ggugaucgag      840

uuccagcaga agaacaaccg gcuccuggag aucacccgcg aguucagcgu gaacgccggg      900

gucaccaccc ccguguccac guacaugcug accaacagcg agcuccuguc ccugaucaac      960

gacaugccga ucaccaacga ccagaagaag cucaugagca acaacgugca gaucguccgg     1020

cagcaguccu acagcaucau gugcaucauc aaggaggagg ugcuggcgua cgugguccag     1080

cugccccucu acggcgugau cgacaccccc ugcuggaagc ugcacacgag cccccugugc     1140

accaccaaca ccaaggaggg guccaacauc ugccucacgc gcaccgaccg gggcugguac     1200

ugcgacaacg ccggcagcgu cuccuucuuc ccgcaggccg agaccugcaa ggugcagagc     1260

aaccgcgugu ucugcgacac caugaacucc cugacgcugc ccagcgaggu aaaccucugc     1320

aacgucgaca ucuucaaccc caaguacgac ugcaagauca ugaccuccaa gaccgacgug     1380

agcuccagcg ugaucaccuc ccugggggcc aucgucagcu gcuacggcaa gacgaagugc     1440

accgccagca acaagaaccg ggggaucauc aagaccuucu ccaacggcug cgacuacgug     1500

agcaacaagg gcguggacac cguguccguc gggaacaccc uguacuacgu gaacaagcag     1560

gagggcaaga gccucuacgu gaagggggag cccaucauca acuucuacga cccgcugguc     1620

uuccccuccg acgaguucga cgcgagcauc ucccagguga acgagaagau caaccagagc     1680

cuggccuuca uccgcaaguc cgacgagcug cuccacaacg ugaacgccgg caagagcacg     1740

accaacauca ugaucaccac caucaucauc gucaucaucg ugauccugcu gucccucauc     1800

gccguggggc ugcugcucua cugcaaggcc cggugaggac uagucccugu ucccagagcc     1860

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     1980

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2035


<210>  320
<211>  2012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  320
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag     1800

auccuucugu aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau     1860

aaaucuggua gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc     1980

ccccaaaggc ucuuuucaga gccaccagaa uu                                   2012


<210>  321
<211>  1953
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  321
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag     1800

auccuucugu aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau     1860

aaaucuggua gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  1953


<210>  322
<211>  1019
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  322
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uaguaauaag gaaacuguau      780

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug      840

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa      900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau      960

cccccccccc cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu      1019


<210>  323
<211>  960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  323
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uaguaauaag gaaacuguau      780

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug      840

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa      900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu      960


<210>  324
<211>  2090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  324
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuaguaauaa ggaaacugua ugaaugucug     1860

ugggcaggaa gugaagagau ccuucuguaa agguuuuugg aauuaugucu gcugaauaau     1920

aaacuuuuuu gaaauaauaa aucugguaga aaaaugagau cuaaaaaaaa aaaaaaaaaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc     2040

cccccccccc cccccccccc ccaaaggcuc uuuucagagc caccagaauu                2090


<210>  325
<211>  2031
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  325
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuaguaauaa ggaaacugua ugaaugucug     1860

ugggcaggaa gugaagagau ccuucuguaa agguuuuugg aauuaugucu gcugaauaau     1920

aaacuuuuuu gaaauaauaa aucugguaga aaaaugagau cuaaaaaaaa aaaaaaaaaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              2031


<210>  326
<211>  2099
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  326
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

cugcucaucc ugaaggccaa cgccaucacc accauccuga cggcggugac cuucugcuuc      240

gccagcggcc agaacaucac cgaggaguuc uaccagucca cgugcagcgc cgucuccaag      300

ggguaccuca gcgcccugcg gaccggcugg uacaccuccg ugaucaccau cgagcugagc      360

aacaucaagg agaacaagug caacgggacc gacgccaagg ugaagcucau caagcaggag      420

cuggacaagu acaagaacgc ggucacggag cugcagcucc ugaugcaguc caccccggca      480

accaacaacc gcgcccggcg cgagcugccc cgguucauga acuacacccu caacaacgcc      540

aagaagacga acgugacccu gagcaagaag cgcaagcggc gcuuccuggg cuuccuccug      600

ggggugggcu ccgccaucgc gagcggcguc gccgugugca aggugcugca ccucgagggg      660

gaggucaaca agaucaagag cgcccugcug uccaccaaca aggccguggu gagccucucc      720

aacggcguca gcgugcugac cuucaaggug cuggaccuca agaacuacau cgacaagcag      780

cugcugccca uccugaacaa gcagagcugc uccaucagca acaucgagac ggugaucgag      840

uuccagcaga agaacaaccg gcuccuggag aucacccgcg aguucagcgu gaacgccggg      900

gucaccaccc ccguguccac guacaugcug accaacagcg agcuccuguc ccugaucaac      960

gacaugccga ucaccaacga ccagaagaag cucaugagca acaacgugca gaucguccgg     1020

cagcaguccu acagcaucau gugcaucauc aaggaggagg ugcuggcgua cgugguccag     1080

cugccccucu acggcgugau cgacaccccc ugcuggaagc ugcacacgag cccccugugc     1140

accaccaaca ccaaggaggg guccaacauc ugccucacgc gcaccgaccg gggcugguac     1200

ugcgacaacg ccggcagcgu cuccuucuuc ccgcaggccg agaccugcaa ggugcagagc     1260

aaccgcgugu ucugcgacac caugaacucc cugacgcugc ccagcgaggu aaaccucugc     1320

aacgucgaca ucuucaaccc caaguacgac ugcaagauca ugaccuccaa gaccgacgug     1380

agcuccagcg ugaucaccuc ccugggggcc aucgucagcu gcuacggcaa gacgaagugc     1440

accgccagca acaagaaccg ggggaucauc aagaccuucu ccaacggcug cgacuacgug     1500

agcaacaagg gcguggacac cguguccguc gggaacaccc uguacuacgu gaacaagcag     1560

gagggcaaga gccucuacgu gaagggggag cccaucauca acuucuacga cccgcugguc     1620

uuccccuccg acgaguucga cgcgagcauc ucccagguga acgagaagau caaccagagc     1680

cuggccuuca uccgcaaguc cgacgagcug cuccacaacg ugaacgccgg caagagcacg     1740

accaacauca ugaucaccac caucaucauc gucaucaucg ugauccugcu gucccucauc     1800

gccguggggc ugcugcucua cugcaaggcc cggugaggac uaguaauaag gaaacuguau     1860

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     1920

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau     2040

cccccccccc cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu      2099


<210>  327
<211>  2028
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  327
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuaguacu ggcugcaucu cccuuuccuc uguccuccau ccuucuccca     1800

ggauggugaa gggggaccug guacccagug auccccaccc caggauccua aaucaugacu     1860

uaccugcuaa uaaaaacuca uuggaaaagu gagaagaucu aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc     1980

cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu                  2028


<210>  328
<211>  1969
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  328
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuaguacu ggcugcaucu cccuuuccuc uguccuccau ccuucuccca     1800

ggauggugaa gggggaccug guacccagug auccccaccc caggauccua aaucaugacu     1860

uaccugcuaa uaaaaacuca uuggaaaagu gagaagaucu aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                 1969


<210>  329
<211>  1035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  329
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uaguacuggc ugcaucuccc      780

uuuccucugu ccuccauccu ucucccagga uggugaaggg ggaccuggua cccagugauc      840

cccaccccag gauccuaaau caugacuuac cugcuaauaa aaacucauug gaaaagugag      900

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      960

aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa ggcucuuuuc     1020

agagccacca gaauu                                                      1035


<210>  330
<211>  976
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  330
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uaguacuggc ugcaucuccc      780

uuuccucugu ccuccauccu ucucccagga uggugaaggg ggaccuggua cccagugauc      840

cccaccccag gauccuaaau caugacuuac cugcuaauaa aaacucauug gaaaagugag      900

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      960

aaaaaaaaaa agaauu                                                      976


<210>  331
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  331
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuaguacugg cugcaucucc cuuuccucug     1860

uccuccaucc uucucccagg auggugaagg gggaccuggu acccagugau ccccacccca     1920

ggauccuaaa ucaugacuua ccugcuaaua aaaacucauu ggaaaaguga gaagaucuaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2040

aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu cagagccacc     2100

agaauu                                                                2106


<210>  332
<211>  2047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  332
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuaguacugg cugcaucucc cuuuccucug     1860

uccuccaucc uucucccagg auggugaagg gggaccuggu acccagugau ccccacccca     1920

ggauccuaaa ucaugacuua ccugcuaaua aaaacucauu ggaaaaguga gaagaucuaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2040

aagaauu                                                               2047


<210>  333
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  333
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

cugcucaucc ugaaggccaa cgccaucacc accauccuga cggcggugac cuucugcuuc      240

gccagcggcc agaacaucac cgaggaguuc uaccagucca cgugcagcgc cgucuccaag      300

ggguaccuca gcgcccugcg gaccggcugg uacaccuccg ugaucaccau cgagcugagc      360

aacaucaagg agaacaagug caacgggacc gacgccaagg ugaagcucau caagcaggag      420

cuggacaagu acaagaacgc ggucacggag cugcagcucc ugaugcaguc caccccggca      480

accaacaacc gcgcccggcg cgagcugccc cgguucauga acuacacccu caacaacgcc      540

aagaagacga acgugacccu gagcaagaag cgcaagcggc gcuuccuggg cuuccuccug      600

ggggugggcu ccgccaucgc gagcggcguc gccgugugca aggugcugca ccucgagggg      660

gaggucaaca agaucaagag cgcccugcug uccaccaaca aggccguggu gagccucucc      720

aacggcguca gcgugcugac cuucaaggug cuggaccuca agaacuacau cgacaagcag      780

cugcugccca uccugaacaa gcagagcugc uccaucagca acaucgagac ggugaucgag      840

uuccagcaga agaacaaccg gcuccuggag aucacccgcg aguucagcgu gaacgccggg      900

gucaccaccc ccguguccac guacaugcug accaacagcg agcuccuguc ccugaucaac      960

gacaugccga ucaccaacga ccagaagaag cucaugagca acaacgugca gaucguccgg     1020

cagcaguccu acagcaucau gugcaucauc aaggaggagg ugcuggcgua cgugguccag     1080

cugccccucu acggcgugau cgacaccccc ugcuggaagc ugcacacgag cccccugugc     1140

accaccaaca ccaaggaggg guccaacauc ugccucacgc gcaccgaccg gggcugguac     1200

ugcgacaacg ccggcagcgu cuccuucuuc ccgcaggccg agaccugcaa ggugcagagc     1260

aaccgcgugu ucugcgacac caugaacucc cugacgcugc ccagcgaggu aaaccucugc     1320

aacgucgaca ucuucaaccc caaguacgac ugcaagauca ugaccuccaa gaccgacgug     1380

agcuccagcg ugaucaccuc ccugggggcc aucgucagcu gcuacggcaa gacgaagugc     1440

accgccagca acaagaaccg ggggaucauc aagaccuucu ccaacggcug cgacuacgug     1500

agcaacaagg gcguggacac cguguccguc gggaacaccc uguacuacgu gaacaagcag     1560

gagggcaaga gccucuacgu gaagggggag cccaucauca acuucuacga cccgcugguc     1620

uuccccuccg acgaguucga cgcgagcauc ucccagguga acgagaagau caaccagagc     1680

cuggccuuca uccgcaaguc cgacgagcug cuccacaacg ugaacgccgg caagagcacg     1740

accaacauca ugaucaccac caucaucauc gucaucaucg ugauccugcu gucccucauc     1800

gccguggggc ugcugcucua cugcaaggcc cggugaggac uaguacuggc ugcaucuccc     1860

uuuccucugu ccuccauccu ucucccagga uggugaaggg ggaccuggua cccagugauc     1920

cccaccccag gauccuaaau caugacuuac cugcuaauaa aaacucauug gaaaagugag     1980

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2040

aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa ggcucuuuuc     2100

agagccacca gaauu                                                      2115


<210>  334
<211>  2244
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  334
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau     1800

uaagagugaa acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc     1860

gcaaccuuuc cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua     1920

gauguuccaa auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac     1980

aaaaguuccc ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa     2040

augaaauuca aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua     2100

aaaaugagca agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc ccccccaaag     2220

gcucuuuuca gagccaccag aauu                                            2244


<210>  335
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  335
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau     1800

uaagagugaa acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc     1860

gcaaccuuuc cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua     1920

gauguuccaa auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac     1980

aaaaguuccc ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa     2040

augaaauuca aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua     2100

aaaaugagca agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa gaauu                                           2185


<210>  336
<211>  1251
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  336
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uagugaaggg aacacccaaa      780

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu      840

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa      900

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu      960

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     1020

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     1080

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     1140

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc     1200

cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau u              1251


<210>  337
<211>  1192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  337
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uagugaaggg aacacccaaa      780

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu      840

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa      900

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu      960

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     1020

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     1080

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     1140

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             1192


<210>  338
<211>  2322
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  338
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuagugaagg gaacacccaa auuuaauuca     1860

gccuuaagca caauuaauua agagugaaac guaauuguac aagcaguugg ucacccacca     1920

uagggcauga ucaacaccgc aaccuuuccu uuuuccccca gugauucuga aaaaccccuc     1980

uucccuucag cuugcuuaga uguuccaaau uuaguaagcu uaaggcggcc uacagaagaa     2040

aaagaaaaaa aaggccacaa aaguucccuc ucacuuucag uaaauaaaau aaaagcagca     2100

acagaaauaa agaaauaaau gaaauucaaa augaaauaaa uauuguguug ugcagcauua     2160

aaaaaucaau aaaaauuaaa aaugagcaag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc     2280

cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu                        2322


<210>  339
<211>  2263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  339
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuagugaagg gaacacccaa auuuaauuca     1860

gccuuaagca caauuaauua agagugaaac guaauuguac aagcaguugg ucacccacca     1920

uagggcauga ucaacaccgc aaccuuuccu uuuuccccca gugauucuga aaaaccccuc     1980

uucccuucag cuugcuuaga uguuccaaau uuaguaagcu uaaggcggcc uacagaagaa     2040

aaagaaaaaa aaggccacaa aaguucccuc ucacuuucag uaaauaaaau aaaagcagca     2100

acagaaauaa agaaauaaau gaaauucaaa augaaauaaa uauuguguug ugcagcauua     2160

aaaaaucaau aaaaauuaaa aaugagcaag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                       2263


<210>  340
<211>  2331
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  340
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

cugcucaucc ugaaggccaa cgccaucacc accauccuga cggcggugac cuucugcuuc      240

gccagcggcc agaacaucac cgaggaguuc uaccagucca cgugcagcgc cgucuccaag      300

ggguaccuca gcgcccugcg gaccggcugg uacaccuccg ugaucaccau cgagcugagc      360

aacaucaagg agaacaagug caacgggacc gacgccaagg ugaagcucau caagcaggag      420

cuggacaagu acaagaacgc ggucacggag cugcagcucc ugaugcaguc caccccggca      480

accaacaacc gcgcccggcg cgagcugccc cgguucauga acuacacccu caacaacgcc      540

aagaagacga acgugacccu gagcaagaag cgcaagcggc gcuuccuggg cuuccuccug      600

ggggugggcu ccgccaucgc gagcggcguc gccgugugca aggugcugca ccucgagggg      660

gaggucaaca agaucaagag cgcccugcug uccaccaaca aggccguggu gagccucucc      720

aacggcguca gcgugcugac cuucaaggug cuggaccuca agaacuacau cgacaagcag      780

cugcugccca uccugaacaa gcagagcugc uccaucagca acaucgagac ggugaucgag      840

uuccagcaga agaacaaccg gcuccuggag aucacccgcg aguucagcgu gaacgccggg      900

gucaccaccc ccguguccac guacaugcug accaacagcg agcuccuguc ccugaucaac      960

gacaugccga ucaccaacga ccagaagaag cucaugagca acaacgugca gaucguccgg     1020

cagcaguccu acagcaucau gugcaucauc aaggaggagg ugcuggcgua cgugguccag     1080

cugccccucu acggcgugau cgacaccccc ugcuggaagc ugcacacgag cccccugugc     1140

accaccaaca ccaaggaggg guccaacauc ugccucacgc gcaccgaccg gggcugguac     1200

ugcgacaacg ccggcagcgu cuccuucuuc ccgcaggccg agaccugcaa ggugcagagc     1260

aaccgcgugu ucugcgacac caugaacucc cugacgcugc ccagcgaggu aaaccucugc     1320

aacgucgaca ucuucaaccc caaguacgac ugcaagauca ugaccuccaa gaccgacgug     1380

agcuccagcg ugaucaccuc ccugggggcc aucgucagcu gcuacggcaa gacgaagugc     1440

accgccagca acaagaaccg ggggaucauc aagaccuucu ccaacggcug cgacuacgug     1500

agcaacaagg gcguggacac cguguccguc gggaacaccc uguacuacgu gaacaagcag     1560

gagggcaaga gccucuacgu gaagggggag cccaucauca acuucuacga cccgcugguc     1620

uuccccuccg acgaguucga cgcgagcauc ucccagguga acgagaagau caaccagagc     1680

cuggccuuca uccgcaaguc cgacgagcug cuccacaacg ugaacgccgg caagagcacg     1740

accaacauca ugaucaccac caucaucauc gucaucaucg ugauccugcu gucccucauc     1800

gccguggggc ugcugcucua cugcaaggcc cggugaggac uagugaaggg aacacccaaa     1860

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu     1920

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa     1980

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu     2040

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     2100

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     2160

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc     2280

cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau u              2331


<210>  341
<211>  2024
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  341
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuagugga agcauuuucc uggcugauua aaagaaauua cucagcuaug     1800

gucaucuguu ccuguuagaa ggcuaugcag cauauuauau acuaugcgca uguuaugaaa     1860

ugcauaauaa aaaauuuuaa aaaaucuaaa agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc     1980

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                      2024


<210>  342
<211>  1965
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  342
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuagugga agcauuuucc uggcugauua aaagaaauua cucagcuaug     1800

gucaucuguu ccuguuagaa ggcuaugcag cauauuauau acuaugcgca uguuaugaaa     1860

ugcauaauaa aaaauuuuaa aaaaucuaaa agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     1965


<210>  343
<211>  1031
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  343
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uaguggaagc auuuuccugg      780

cugauuaaaa gaaauuacuc agcuaugguc aucuguuccu guuagaaggc uaugcagcau      840

auuauauacu augcgcaugu uaugaaaugc auaauaaaaa auuuuaaaaa aucuaaaaga      900

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      960

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag     1020

ccaccagaau u                                                          1031


<210>  344
<211>  972
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  344
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uaguggaagc auuuuccugg      780

cugauuaaaa gaaauuacuc agcuaugguc aucuguuccu guuagaaggc uaugcagcau      840

auuauauacu augcgcaugu uaugaaaugc auaauaaaaa auuuuaaaaa aucuaaaaga      900

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      960

aaaaaaagaa uu                                                          972


<210>  345
<211>  2102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  345
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuaguggaag cauuuuccug gcugauuaaa     1860

agaaauuacu cagcuauggu caucuguucc uguuagaagg cuaugcagca uauuauauac     1920

uaugcgcaug uuaugaaaug cauaauaaaa aauuuuaaaa aaucuaaaag aucuaaaaaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug     2040

cauccccccc cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa     2100

uu                                                                    2102


<210>  346
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  346
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuaguggaag cauuuuccug gcugauuaaa     1860

agaaauuacu cagcuauggu caucuguucc uguuagaagg cuaugcagca uauuauauac     1920

uaugcgcaug uuaugaaaug cauaauaaaa aauuuuaaaa aaucuaaaag aucuaaaaaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     2040

auu                                                                   2043


<210>  347
<211>  2111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  347
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

cugcucaucc ugaaggccaa cgccaucacc accauccuga cggcggugac cuucugcuuc      240

gccagcggcc agaacaucac cgaggaguuc uaccagucca cgugcagcgc cgucuccaag      300

ggguaccuca gcgcccugcg gaccggcugg uacaccuccg ugaucaccau cgagcugagc      360

aacaucaagg agaacaagug caacgggacc gacgccaagg ugaagcucau caagcaggag      420

cuggacaagu acaagaacgc ggucacggag cugcagcucc ugaugcaguc caccccggca      480

accaacaacc gcgcccggcg cgagcugccc cgguucauga acuacacccu caacaacgcc      540

aagaagacga acgugacccu gagcaagaag cgcaagcggc gcuuccuggg cuuccuccug      600

ggggugggcu ccgccaucgc gagcggcguc gccgugugca aggugcugca ccucgagggg      660

gaggucaaca agaucaagag cgcccugcug uccaccaaca aggccguggu gagccucucc      720

aacggcguca gcgugcugac cuucaaggug cuggaccuca agaacuacau cgacaagcag      780

cugcugccca uccugaacaa gcagagcugc uccaucagca acaucgagac ggugaucgag      840

uuccagcaga agaacaaccg gcuccuggag aucacccgcg aguucagcgu gaacgccggg      900

gucaccaccc ccguguccac guacaugcug accaacagcg agcuccuguc ccugaucaac      960

gacaugccga ucaccaacga ccagaagaag cucaugagca acaacgugca gaucguccgg     1020

cagcaguccu acagcaucau gugcaucauc aaggaggagg ugcuggcgua cgugguccag     1080

cugccccucu acggcgugau cgacaccccc ugcuggaagc ugcacacgag cccccugugc     1140

accaccaaca ccaaggaggg guccaacauc ugccucacgc gcaccgaccg gggcugguac     1200

ugcgacaacg ccggcagcgu cuccuucuuc ccgcaggccg agaccugcaa ggugcagagc     1260

aaccgcgugu ucugcgacac caugaacucc cugacgcugc ccagcgaggu aaaccucugc     1320

aacgucgaca ucuucaaccc caaguacgac ugcaagauca ugaccuccaa gaccgacgug     1380

agcuccagcg ugaucaccuc ccugggggcc aucgucagcu gcuacggcaa gacgaagugc     1440

accgccagca acaagaaccg ggggaucauc aagaccuucu ccaacggcug cgacuacgug     1500

agcaacaagg gcguggacac cguguccguc gggaacaccc uguacuacgu gaacaagcag     1560

gagggcaaga gccucuacgu gaagggggag cccaucauca acuucuacga cccgcugguc     1620

uuccccuccg acgaguucga cgcgagcauc ucccagguga acgagaagau caaccagagc     1680

cuggccuuca uccgcaaguc cgacgagcug cuccacaacg ugaacgccgg caagagcacg     1740

accaacauca ugaucaccac caucaucauc gucaucaucg ugauccugcu gucccucauc     1800

gccguggggc ugcugcucua cugcaaggcc cggugaggac uaguggaagc auuuuccugg     1860

cugauuaaaa gaaauuacuc agcuaugguc aucuguuccu guuagaaggc uaugcagcau     1920

auuauauacu augcgcaugu uaugaaaugc auaauaaaaa auuuuaaaaa aucuaaaaga     1980

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2040

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag     2100

ccaccagaau u                                                          2111


<210>  348
<211>  1961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  348
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu     1800

gccaaauaaa caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc     1920

cccccccccc cccaaaggcu cuuuucagag ccaccagaau u                         1961


<210>  349
<211>  1902
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  349
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggug      180

ccccaggccc uccuguucgu cccgcugcug guguuccccc ucugcuucgg caaguucccc      240

aucuacacca uccccgacaa gcuggggccg uggagcccca ucgacaucca ccaccugucc      300

ugccccaaca accucguggu cgaggacgag ggcugcacca accugagcgg guucuccuac      360

auggagcuga aggugggcua caucagcgcc aucaagauga acggguucac gugcaccggc      420

guggucaccg aggcggagac cuacacgaac uucgugggcu acgugaccac caccuucaag      480

cggaagcacu uccgccccac gccggacgcc ugccgggccg ccuacaacug gaagauggcc      540

ggggaccccc gcuacgagga gucccuccac aaccccuacc ccgacuacca cuggcugcgg      600

accgucaaga ccaccaagga gagccuggug aucaucuccc cgagcguggc ggaccucgac      660

cccuacgacc gcucccugca cagccggguc uuccccggcg ggaacugcuc cggcguggcc      720

gugagcucca cguacugcag caccaaccac gacuacacca ucuggaugcc cgagaacccg      780

cgccugggga uguccugcga caucuucacc aacagccggg gcaagcgcgc cuccaagggc      840

agcgagacgu gcggguucgu cgacgagcgg ggccucuaca agucccugaa gggggccugc      900

aagcugaagc ucugcggcgu gcugggccug cgccucaugg acgggaccug gguggcgaug      960

cagaccagca acgagaccaa guggugcccc cccggccagc uggucaaccu gcacgacuuc     1020

cggagcgacg agaucgagca ccucguggug gaggagcugg ucaagaagcg cgaggagugc     1080

cuggacgccc ucgaguccau caugacgacc aagagcgugu ccuuccggcg ccugagccac     1140

cuccggaagc uggugcccgg guucggcaag gccuacacca ucuucaacaa gacccugaug     1200

gaggccgacg cccacuacaa guccguccgc acguggaacg agaucauccc gagcaagggg     1260

ugccugcggg ugggcggccg cugccacccc cacgucaacg ggguguucuu caacggcauc     1320

auccucgggc ccgacggcaa cgugcugauc cccgagaugc aguccagccu gcuccagcag     1380

cacauggagc ugcuggucuc cagcgugauc ccgcucaugc acccccuggc ggaccccucc     1440

accguguuca agaacgggga cgaggccgag gacuucgucg aggugcaccu ccccgacgug     1500

cacgagcgga ucagcggcgu cgaccugggc cugccgaacu gggggaagua cgugcugcuc     1560

uccgccggcg cccugaccgc ccugaugcug aucaucuucc ucaugaccug cuggcgccgg     1620

gugaaccgga gcgagcccac gcagcacaac cugcgcggga ccggccggga ggucuccgug     1680

accccgcaga gcgggaagau caucuccagc ugggaguccu acaagagcgg cggcgagacc     1740

gggcugugag gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu     1800

gccaaauaaa caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                        1902


<210>  350
<211>  968
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  350
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uaguguccac cugucccucc      780

ugggcugcug gauugucucg uuuuccugcc aaauaaacag gaucagcgcu uuacagaucu      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca      960

ccagaauu                                                               968


<210>  351
<211>  909
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  351
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccaugggc      180

gugcacgagu gccccgccug gcuguggcuc cugcugagcc uccugucccu gccgcucggg      240

cugcccgucc ugggcgcccc cccgcggcuc aucugcgaca gccgcgugcu ggagcgguac      300

cugcucgagg cgaaggaggc cgagaacauc accaccgggu gcgccgagca cugcucccug      360

aacgagaaca ucacggugcc ggacaccaag gucaacuucu acgccuggaa gcgcauggag      420

gugggccagc aggccgugga ggucuggcag gggcuggcgc uccugagcga ggccgugcug      480

cggggccagg cccuccuggu gaacuccagc cagcccuggg agccccugca gcuccacguc      540

gacaaggccg uguccggccu gcgcagccug accacccucc ugcgggcgcu gggggcccag      600

aaggaggcca ucuccccccc ggacgccgcc agcgcggccc cgcuccgcac gaucaccgcc      660

gacaccuucc ggaagcuguu ccgcguguac uccaacuucc ugcggggcaa gcucaagcug      720

uacaccgggg aggccugccg cacgggcgac cggugaggac uaguguccac cugucccucc      780

ugggcugcug gauugucucg uuuuccugcc aaauaaacag gaucagcgcu uuacagaucu      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaagaauu                                                              909


<210>  352
<211>  2039
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  352
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuagugucca ccugucccuc cugggcugcu     1860

ggauugucuc guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau     1980

cccccccccc cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu      2039


<210>  353
<211>  1980
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  353
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

gacgccaaga acaucaagaa gggcccggcg cccuucuacc cgcuggagga cgggaccgcc      240

ggcgagcagc uccacaaggc caugaagcgg uacgcccugg ugccgggcac gaucgccuuc      300

accgacgccc acaucgaggu cgacaucacc uacgcggagu acuucgagau gagcgugcgc      360

cuggccgagg ccaugaagcg guacggccug aacaccaacc accggaucgu ggugugcucg      420

gagaacagcc ugcaguucuu caugccggug cugggcgccc ucuucaucgg cguggccguc      480

gccccggcga acgacaucua caacgagcgg gagcugcuga acagcauggg gaucagccag      540

ccgaccgugg uguucgugag caagaagggc cugcagaaga uccugaacgu gcagaagaag      600

cugcccauca uccagaagau caucaucaug gacagcaaga ccgacuacca gggcuuccag      660

ucgauguaca cguucgugac cagccaccuc ccgccgggcu ucaacgagua cgacuucguc      720

ccggagagcu ucgaccggga caagaccauc gcccugauca ugaacagcag cggcagcacc      780

ggccugccga aggggguggc ccugccgcac cggaccgccu gcgugcgcuu cucgcacgcc      840

cgggacccca ucuucggcaa ccagaucauc ccggacaccg ccauccugag cguggugccg      900

uuccaccacg gcuucggcau guucacgacc cugggcuacc ucaucugcgg cuuccgggug      960

guccugaugu accgguucga ggaggagcug uuccugcgga gccugcagga cuacaagauc     1020

cagagcgcgc ugcucgugcc gacccuguuc agcuucuucg ccaagagcac ccugaucgac     1080

aaguacgacc ugucgaaccu gcacgagauc gccagcgggg gcgccccgcu gagcaaggag     1140

gugggcgagg ccguggccaa gcgguuccac cucccgggca uccgccaggg cuacggccug     1200

accgagacca cgagcgcgau ccugaucacc cccgaggggg acgacaagcc gggcgccgug     1260

ggcaaggugg ucccguucuu cgaggccaag gugguggacc uggacaccgg caagacccug     1320

ggcgugaacc agcggggcga gcugugcgug cgggggccga ugaucaugag cggcuacgug     1380

aacaacccgg aggccaccaa cgcccucauc gacaaggacg gcuggcugca cagcggcgac     1440

aucgccuacu gggacgagga cgagcacuuc uucaucgucg accggcugaa gucgcugauc     1500

aaguacaagg gcuaccaggu ggcgccggcc gagcuggaga gcauccugcu ccagcacccc     1560

aacaucuucg acgccggcgu ggccgggcug ccggacgacg acgccggcga gcugccggcc     1620

gcgguggugg ugcuggagca cggcaagacc augacggaga aggagaucgu cgacuacgug     1680

gccagccagg ugaccaccgc caagaagcug cggggcggcg ugguguucgu ggacgagguc     1740

ccgaagggcc ugaccgggaa gcucgacgcc cggaagaucc gcgagauccu gaucaaggcc     1800

aagaagggcg gcaagaucgc cgugugagga cuagugucca ccugucccuc cugggcugcu     1860

ggauugucuc guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     1980


<210>  354
<211>  2048
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  354
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggag      180

cugcucaucc ugaaggccaa cgccaucacc accauccuga cggcggugac cuucugcuuc      240

gccagcggcc agaacaucac cgaggaguuc uaccagucca cgugcagcgc cgucuccaag      300

ggguaccuca gcgcccugcg gaccggcugg uacaccuccg ugaucaccau cgagcugagc      360

aacaucaagg agaacaagug caacgggacc gacgccaagg ugaagcucau caagcaggag      420

cuggacaagu acaagaacgc ggucacggag cugcagcucc ugaugcaguc caccccggca      480

accaacaacc gcgcccggcg cgagcugccc cgguucauga acuacacccu caacaacgcc      540

aagaagacga acgugacccu gagcaagaag cgcaagcggc gcuuccuggg cuuccuccug      600

ggggugggcu ccgccaucgc gagcggcguc gccgugugca aggugcugca ccucgagggg      660

gaggucaaca agaucaagag cgcccugcug uccaccaaca aggccguggu gagccucucc      720

aacggcguca gcgugcugac cuucaaggug cuggaccuca agaacuacau cgacaagcag      780

cugcugccca uccugaacaa gcagagcugc uccaucagca acaucgagac ggugaucgag      840

uuccagcaga agaacaaccg gcuccuggag aucacccgcg aguucagcgu gaacgccggg      900

gucaccaccc ccguguccac guacaugcug accaacagcg agcuccuguc ccugaucaac      960

gacaugccga ucaccaacga ccagaagaag cucaugagca acaacgugca gaucguccgg     1020

cagcaguccu acagcaucau gugcaucauc aaggaggagg ugcuggcgua cgugguccag     1080

cugccccucu acggcgugau cgacaccccc ugcuggaagc ugcacacgag cccccugugc     1140

accaccaaca ccaaggaggg guccaacauc ugccucacgc gcaccgaccg gggcugguac     1200

ugcgacaacg ccggcagcgu cuccuucuuc ccgcaggccg agaccugcaa ggugcagagc     1260

aaccgcgugu ucugcgacac caugaacucc cugacgcugc ccagcgaggu aaaccucugc     1320

aacgucgaca ucuucaaccc caaguacgac ugcaagauca ugaccuccaa gaccgacgug     1380

agcuccagcg ugaucaccuc ccugggggcc aucgucagcu gcuacggcaa gacgaagugc     1440

accgccagca acaagaaccg ggggaucauc aagaccuucu ccaacggcug cgacuacgug     1500

agcaacaagg gcguggacac cguguccguc gggaacaccc uguacuacgu gaacaagcag     1560

gagggcaaga gccucuacgu gaagggggag cccaucauca acuucuacga cccgcugguc     1620

uuccccuccg acgaguucga cgcgagcauc ucccagguga acgagaagau caaccagagc     1680

cuggccuuca uccgcaaguc cgacgagcug cuccacaacg ugaacgccgg caagagcacg     1740

accaacauca ugaucaccac caucaucauc gucaucaucg ugauccugcu gucccucauc     1800

gccguggggc ugcugcucua cugcaaggcc cggugaggac uaguguccac cugucccucc     1860

ugggcugcug gauugucucg uuuuccugcc aaauaaacag gaucagcgcu uuacagaucu     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca     2040

ccagaauu                                                              2048


<210>  355
<211>  1904
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  355
gggagaauau aagcguuggc ggagcgucgg uuguagcacu cugcgcgccc gcucuucugc       60

ugcuguuugu cuacuuccuc cugcuucccc gccgccgccg ccgccaucaa gcuuaccaug      120

gugccccagg cccuccuguu cgucccgcug cugguguucc cccucugcuu cggcaaguuc      180

cccaucuaca ccauccccga caagcugggg ccguggagcc ccaucgacau ccaccaccug      240

uccugcccca acaaccucgu ggucgaggac gagggcugca ccaaccugag cggguucucc      300

uacauggagc ugaagguggg cuacaucagc gccaucaaga ugaacggguu cacgugcacc      360

ggcgugguca ccgaggcgga gaccuacacg aacuucgugg gcuacgugac caccaccuuc      420

aagcggaagc acuuccgccc cacgccggac gccugccggg ccgccuacaa cuggaagaug      480

gccggggacc cccgcuacga ggagucccuc cacaaccccu accccgacua ccacuggcug      540

cggaccguca agaccaccaa ggagagccug gugaucaucu ccccgagcgu ggcggaccuc      600

gaccccuacg accgcucccu gcacagccgg gucuuccccg gcgggaacug cuccggcgug      660

gccgugagcu ccacguacug cagcaccaac cacgacuaca ccaucuggau gcccgagaac      720

ccgcgccugg ggauguccug cgacaucuuc accaacagcc ggggcaagcg cgccuccaag      780

ggcagcgaga cgugcggguu cgucgacgag cggggccucu acaagucccu gaagggggcc      840

ugcaagcuga agcucugcgg cgugcugggc cugcgccuca uggacgggac cuggguggcg      900

augcagacca gcaacgagac caaguggugc ccccccggcc agcuggucaa ccugcacgac      960

uuccggagcg acgagaucga gcaccucgug guggaggagc uggucaagaa gcgcgaggag     1020

ugccuggacg cccucgaguc caucaugacg accaagagcg uguccuuccg gcgccugagc     1080

caccuccgga agcuggugcc cggguucggc aaggccuaca ccaucuucaa caagacccug     1140

auggaggccg acgcccacua caaguccguc cgcacgugga acgagaucau cccgagcaag     1200

gggugccugc gggugggcgg ccgcugccac ccccacguca acgggguguu cuucaacggc     1260

aucauccucg ggcccgacgg caacgugcug auccccgaga ugcaguccag ccugcuccag     1320

cagcacaugg agcugcuggu cuccagcgug aucccgcuca ugcacccccu ggcggacccc     1380

uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg ucgaggugca ccuccccgac     1440

gugcacgagc ggaucagcgg cgucgaccug ggccugccga acugggggaa guacgugcug     1500

cucuccgccg gcgcccugac cgcccugaug cugaucaucu uccucaugac cugcuggcgc     1560

cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg ggaccggccg ggaggucucc     1620

gugaccccgc agagcgggaa gaucaucucc agcugggagu ccuacaagag cggcggcgag     1680

accgggcugu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu     1740

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc     1860

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                      1904


<210>  356
<211>  1845
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  356
gggagaauau aagcguuggc ggagcgucgg uuguagcacu cugcgcgccc gcucuucugc       60

ugcuguuugu cuacuuccuc cugcuucccc gccgccgccg ccgccaucaa gcuuaccaug      120

gugccccagg cccuccuguu cgucccgcug cugguguucc cccucugcuu cggcaaguuc      180

cccaucuaca ccauccccga caagcugggg ccguggagcc ccaucgacau ccaccaccug      240

uccugcccca acaaccucgu ggucgaggac gagggcugca ccaaccugag cggguucucc      300

uacauggagc ugaagguggg cuacaucagc gccaucaaga ugaacggguu cacgugcacc      360

ggcgugguca ccgaggcgga gaccuacacg aacuucgugg gcuacgugac caccaccuuc      420

aagcggaagc acuuccgccc cacgccggac gccugccggg ccgccuacaa cuggaagaug      480

gccggggacc cccgcuacga ggagucccuc cacaaccccu accccgacua ccacuggcug      540

cggaccguca agaccaccaa ggagagccug gugaucaucu ccccgagcgu ggcggaccuc      600

gaccccuacg accgcucccu gcacagccgg gucuuccccg gcgggaacug cuccggcgug      660

gccgugagcu ccacguacug cagcaccaac cacgacuaca ccaucuggau gcccgagaac      720

ccgcgccugg ggauguccug cgacaucuuc accaacagcc ggggcaagcg cgccuccaag      780

ggcagcgaga cgugcggguu cgucgacgag cggggccucu acaagucccu gaagggggcc      840

ugcaagcuga agcucugcgg cgugcugggc cugcgccuca uggacgggac cuggguggcg      900

augcagacca gcaacgagac caaguggugc ccccccggcc agcuggucaa ccugcacgac      960

uuccggagcg acgagaucga gcaccucgug guggaggagc uggucaagaa gcgcgaggag     1020

ugccuggacg cccucgaguc caucaugacg accaagagcg uguccuuccg gcgccugagc     1080

caccuccgga agcuggugcc cggguucggc aaggccuaca ccaucuucaa caagacccug     1140

auggaggccg acgcccacua caaguccguc cgcacgugga acgagaucau cccgagcaag     1200

gggugccugc gggugggcgg ccgcugccac ccccacguca acgggguguu cuucaacggc     1260

aucauccucg ggcccgacgg caacgugcug auccccgaga ugcaguccag ccugcuccag     1320

cagcacaugg agcugcuggu cuccagcgug aucccgcuca ugcacccccu ggcggacccc     1380

uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg ucgaggugca ccuccccgac     1440

gugcacgagc ggaucagcgg cgucgaccug ggccugccga acugggggaa guacgugcug     1500

cucuccgccg gcgcccugac cgcccugaug cugaucaucu uccucaugac cugcuggcgc     1560

cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg ggaccggccg ggaggucucc     1620

gugaccccgc agagcgggaa gaucaucucc agcugggagu ccuacaagag cggcggcgag     1680

accgggcugu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu     1740

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     1845


<210>  357
<211>  911
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  357
gggagaauau aagcguuggc ggagcgucgg uuguagcacu cugcgcgccc gcucuucugc       60

ugcuguuugu cuacuuccuc cugcuucccc gccgccgccg ccgccaucaa gcuuaccaug      120

ggcgugcacg agugccccgc cuggcugugg cuccugcuga gccuccuguc ccugccgcuc      180

gggcugcccg uccugggcgc ccccccgcgg cucaucugcg acagccgcgu gcuggagcgg      240

uaccugcucg aggcgaagga ggccgagaac aucaccaccg ggugcgccga gcacugcucc      300

cugaacgaga acaucacggu gccggacacc aaggucaacu ucuacgccug gaagcgcaug      360

gaggugggcc agcaggccgu ggaggucugg caggggcugg cgcuccugag cgaggccgug      420

cugcggggcc aggcccuccu ggugaacucc agccagcccu gggagccccu gcagcuccac      480

gucgacaagg ccguguccgg ccugcgcagc cugaccaccc uccugcgggc gcugggggcc      540

cagaaggagg ccaucucccc cccggacgcc gccagcgcgg ccccgcuccg cacgaucacc      600

gccgacaccu uccggaagcu guuccgcgug uacuccaacu uccugcgggg caagcucaag      660

cuguacaccg gggaggccug ccgcacgggc gaccggugag gacuaguguc caccuguccc      720

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga      780

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag      900

ccaccagaau u                                                           911


<210>  358
<211>  852
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  358
gggagaauau aagcguuggc ggagcgucgg uuguagcacu cugcgcgccc gcucuucugc       60

ugcuguuugu cuacuuccuc cugcuucccc gccgccgccg ccgccaucaa gcuuaccaug      120

ggcgugcacg agugccccgc cuggcugugg cuccugcuga gccuccuguc ccugccgcuc      180

gggcugcccg uccugggcgc ccccccgcgg cucaucugcg acagccgcgu gcuggagcgg      240

uaccugcucg aggcgaagga ggccgagaac aucaccaccg ggugcgccga gcacugcucc      300

cugaacgaga acaucacggu gccggacacc aaggucaacu ucuacgccug gaagcgcaug      360

gaggugggcc agcaggccgu ggaggucugg caggggcugg cgcuccugag cgaggccgug      420

cugcggggcc aggcccuccu ggugaacucc agccagcccu gggagccccu gcagcuccac      480

gucgacaagg ccguguccgg ccugcgcagc cugaccaccc uccugcgggc gcugggggcc      540

cagaaggagg ccaucucccc cccggacgcc gccagcgcgg ccccgcuccg cacgaucacc      600

gccgacaccu uccggaagcu guuccgcgug uacuccaacu uccugcgggg caagcucaag      660

cuguacaccg gggaggccug ccgcacgggc gaccggugag gacuaguguc caccuguccc      720

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga      780

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaagaa uu                                                          852


<210>  359
<211>  1982
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  359
gggagaauau aagcguuggc ggagcgucgg uuguagcacu cugcgcgccc gcucuucugc       60

ugcuguuugu cuacuuccuc cugcuucccc gccgccgccg ccgccaucaa gcuuaccaug      120

gaggacgcca agaacaucaa gaagggcccg gcgcccuucu acccgcugga ggacgggacc      180

gccggcgagc agcuccacaa ggccaugaag cgguacgccc uggugccggg cacgaucgcc      240

uucaccgacg cccacaucga ggucgacauc accuacgcgg aguacuucga gaugagcgug      300

cgccuggccg aggccaugaa gcgguacggc cugaacacca accaccggau cguggugugc      360

ucggagaaca gccugcaguu cuucaugccg gugcugggcg cccucuucau cggcguggcc      420

gucgccccgg cgaacgacau cuacaacgag cgggagcugc ugaacagcau ggggaucagc      480

cagccgaccg ugguguucgu gagcaagaag ggccugcaga agauccugaa cgugcagaag      540

aagcugccca ucauccagaa gaucaucauc auggacagca agaccgacua ccagggcuuc      600

cagucgaugu acacguucgu gaccagccac cucccgccgg gcuucaacga guacgacuuc      660

gucccggaga gcuucgaccg ggacaagacc aucgcccuga ucaugaacag cagcggcagc      720

accggccugc cgaagggggu ggcccugccg caccggaccg ccugcgugcg cuucucgcac      780

gcccgggacc ccaucuucgg caaccagauc aucccggaca ccgccauccu gagcguggug      840

ccguuccacc acggcuucgg cauguucacg acccugggcu accucaucug cggcuuccgg      900

gugguccuga uguaccgguu cgaggaggag cuguuccugc ggagccugca ggacuacaag      960

auccagagcg cgcugcucgu gccgacccug uucagcuucu ucgccaagag cacccugauc     1020

gacaaguacg accugucgaa ccugcacgag aucgccagcg ggggcgcccc gcugagcaag     1080

gaggugggcg aggccguggc caagcgguuc caccucccgg gcauccgcca gggcuacggc     1140

cugaccgaga ccacgagcgc gauccugauc acccccgagg gggacgacaa gccgggcgcc     1200

gugggcaagg uggucccguu cuucgaggcc aagguggugg accuggacac cggcaagacc     1260

cugggcguga accagcgggg cgagcugugc gugcgggggc cgaugaucau gagcggcuac     1320

gugaacaacc cggaggccac caacgcccuc aucgacaagg acggcuggcu gcacagcggc     1380

gacaucgccu acugggacga ggacgagcac uucuucaucg ucgaccggcu gaagucgcug     1440

aucaaguaca agggcuacca gguggcgccg gccgagcugg agagcauccu gcuccagcac     1500

cccaacaucu ucgacgccgg cguggccggg cugccggacg acgacgccgg cgagcugccg     1560

gccgcggugg uggugcugga gcacggcaag accaugacgg agaaggagau cgucgacuac     1620

guggccagcc aggugaccac cgccaagaag cugcggggcg gcgugguguu cguggacgag     1680

gucccgaagg gccugaccgg gaagcucgac gcccggaaga uccgcgagau ccugaucaag     1740

gccaagaagg gcggcaagau cgccguguga ggacuagugu ccaccugucc cuccugggcu     1800

gcuggauugu cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug     1920

cauccccccc cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa     1980

uu                                                                    1982


<210>  360
<211>  1923
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  360
gggagaauau aagcguuggc ggagcgucgg uuguagcacu cugcgcgccc gcucuucugc       60

ugcuguuugu cuacuuccuc cugcuucccc gccgccgccg ccgccaucaa gcuuaccaug      120

gaggacgcca agaacaucaa gaagggcccg gcgcccuucu acccgcugga ggacgggacc      180

gccggcgagc agcuccacaa ggccaugaag cgguacgccc uggugccggg cacgaucgcc      240

uucaccgacg cccacaucga ggucgacauc accuacgcgg aguacuucga gaugagcgug      300

cgccuggccg aggccaugaa gcgguacggc cugaacacca accaccggau cguggugugc      360

ucggagaaca gccugcaguu cuucaugccg gugcugggcg cccucuucau cggcguggcc      420

gucgccccgg cgaacgacau cuacaacgag cgggagcugc ugaacagcau ggggaucagc      480

cagccgaccg ugguguucgu gagcaagaag ggccugcaga agauccugaa cgugcagaag      540

aagcugccca ucauccagaa gaucaucauc auggacagca agaccgacua ccagggcuuc      600

cagucgaugu acacguucgu gaccagccac cucccgccgg gcuucaacga guacgacuuc      660

gucccggaga gcuucgaccg ggacaagacc aucgcccuga ucaugaacag cagcggcagc      720

accggccugc cgaagggggu ggcccugccg caccggaccg ccugcgugcg cuucucgcac      780

gcccgggacc ccaucuucgg caaccagauc aucccggaca ccgccauccu gagcguggug      840

ccguuccacc acggcuucgg cauguucacg acccugggcu accucaucug cggcuuccgg      900

gugguccuga uguaccgguu cgaggaggag cuguuccugc ggagccugca ggacuacaag      960

auccagagcg cgcugcucgu gccgacccug uucagcuucu ucgccaagag cacccugauc     1020

gacaaguacg accugucgaa ccugcacgag aucgccagcg ggggcgcccc gcugagcaag     1080

gaggugggcg aggccguggc caagcgguuc caccucccgg gcauccgcca gggcuacggc     1140

cugaccgaga ccacgagcgc gauccugauc acccccgagg gggacgacaa gccgggcgcc     1200

gugggcaagg uggucccguu cuucgaggcc aagguggugg accuggacac cggcaagacc     1260

cugggcguga accagcgggg cgagcugugc gugcgggggc cgaugaucau gagcggcuac     1320

gugaacaacc cggaggccac caacgcccuc aucgacaagg acggcuggcu gcacagcggc     1380

gacaucgccu acugggacga ggacgagcac uucuucaucg ucgaccggcu gaagucgcug     1440

aucaaguaca agggcuacca gguggcgccg gccgagcugg agagcauccu gcuccagcac     1500

cccaacaucu ucgacgccgg cguggccggg cugccggacg acgacgccgg cgagcugccg     1560

gccgcggugg uggugcugga gcacggcaag accaugacgg agaaggagau cgucgacuac     1620

guggccagcc aggugaccac cgccaagaag cugcggggcg gcgugguguu cguggacgag     1680

gucccgaagg gccugaccgg gaagcucgac gcccggaaga uccgcgagau ccugaucaag     1740

gccaagaagg gcggcaagau cgccguguga ggacuagugu ccaccugucc cuccugggcu     1800

gcuggauugu cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     1920

auu                                                                   1923


<210>  361
<211>  1991
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  361
gggagaauau aagcguuggc ggagcgucgg uuguagcacu cugcgcgccc gcucuucugc       60

ugcuguuugu cuacuuccuc cugcuucccc gccgccgccg ccgccaucaa gcuuaccaug      120

gagcugcuca uccugaaggc caacgccauc accaccaucc ugacggcggu gaccuucugc      180

uucgccagcg gccagaacau caccgaggag uucuaccagu ccacgugcag cgccgucucc      240

aagggguacc ucagcgcccu gcggaccggc ugguacaccu ccgugaucac caucgagcug      300

agcaacauca aggagaacaa gugcaacggg accgacgcca aggugaagcu caucaagcag      360

gagcuggaca aguacaagaa cgcggucacg gagcugcagc uccugaugca guccaccccg      420

gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca ugaacuacac ccucaacaac      480

gccaagaaga cgaacgugac ccugagcaag aagcgcaagc ggcgcuuccu gggcuuccuc      540

cugggggugg gcuccgccau cgcgagcggc gucgccgugu gcaaggugcu gcaccucgag      600

ggggagguca acaagaucaa gagcgcccug cuguccacca acaaggccgu ggugagccuc      660

uccaacggcg ucagcgugcu gaccuucaag gugcuggacc ucaagaacua caucgacaag      720

cagcugcugc ccauccugaa caagcagagc ugcuccauca gcaacaucga gacggugauc      780

gaguuccagc agaagaacaa ccggcuccug gagaucaccc gcgaguucag cgugaacgcc      840

ggggucacca cccccguguc cacguacaug cugaccaaca gcgagcuccu gucccugauc      900

aacgacaugc cgaucaccaa cgaccagaag aagcucauga gcaacaacgu gcagaucguc      960

cggcagcagu ccuacagcau caugugcauc aucaaggagg aggugcuggc guacgugguc     1020

cagcugcccc ucuacggcgu gaucgacacc cccugcugga agcugcacac gagcccccug     1080

ugcaccacca acaccaagga gggguccaac aucugccuca cgcgcaccga ccggggcugg     1140

uacugcgaca acgccggcag cgucuccuuc uucccgcagg ccgagaccug caaggugcag     1200

agcaaccgcg uguucugcga caccaugaac ucccugacgc ugcccagcga gguaaaccuc     1260

ugcaacgucg acaucuucaa ccccaaguac gacugcaaga ucaugaccuc caagaccgac     1320

gugagcucca gcgugaucac cucccugggg gccaucguca gcugcuacgg caagacgaag     1380

ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu ucuccaacgg cugcgacuac     1440

gugagcaaca agggcgugga caccgugucc gucgggaaca cccuguacua cgugaacaag     1500

caggagggca agagccucua cgugaagggg gagcccauca ucaacuucua cgacccgcug     1560

gucuuccccu ccgacgaguu cgacgcgagc aucucccagg ugaacgagaa gaucaaccag     1620

agccuggccu ucauccgcaa guccgacgag cugcuccaca acgugaacgc cggcaagagc     1680

acgaccaaca ucaugaucac caccaucauc aucgucauca ucgugauccu gcugucccuc     1740

aucgccgugg ggcugcugcu cuacugcaag gcccggugag gacuaguguc caccuguccc     1800

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     1860

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag     1980

ccaccagaau u                                                          1991


<210>  362
<211>  2024
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  362
gggagacgga gacggccugc aggaccugcu cucucagccc ucagccgagg ccuacgccga       60

gccgagugcg cagccgacga ccgggaggag ccgcagccuu caacucugag guacugugau      120

ccgcgcugcc cgccgggccg ccccaguccg cugcugcggc accuccuucc cucgcgcccu      180

cuucgcucgc cagcgccuuc ccugugagcc ugcgucaccg cggccgccaa gcuuaccaug      240

gugccccagg cccuccuguu cgucccgcug cugguguucc cccucugcuu cggcaaguuc      300

cccaucuaca ccauccccga caagcugggg ccguggagcc ccaucgacau ccaccaccug      360

uccugcccca acaaccucgu ggucgaggac gagggcugca ccaaccugag cggguucucc      420

uacauggagc ugaagguggg cuacaucagc gccaucaaga ugaacggguu cacgugcacc      480

ggcgugguca ccgaggcgga gaccuacacg aacuucgugg gcuacgugac caccaccuuc      540

aagcggaagc acuuccgccc cacgccggac gccugccggg ccgccuacaa cuggaagaug      600

gccggggacc cccgcuacga ggagucccuc cacaaccccu accccgacua ccacuggcug      660

cggaccguca agaccaccaa ggagagccug gugaucaucu ccccgagcgu ggcggaccuc      720

gaccccuacg accgcucccu gcacagccgg gucuuccccg gcgggaacug cuccggcgug      780

gccgugagcu ccacguacug cagcaccaac cacgacuaca ccaucuggau gcccgagaac      840

ccgcgccugg ggauguccug cgacaucuuc accaacagcc ggggcaagcg cgccuccaag      900

ggcagcgaga cgugcggguu cgucgacgag cggggccucu acaagucccu gaagggggcc      960

ugcaagcuga agcucugcgg cgugcugggc cugcgccuca uggacgggac cuggguggcg     1020

augcagacca gcaacgagac caaguggugc ccccccggcc agcuggucaa ccugcacgac     1080

uuccggagcg acgagaucga gcaccucgug guggaggagc uggucaagaa gcgcgaggag     1140

ugccuggacg cccucgaguc caucaugacg accaagagcg uguccuuccg gcgccugagc     1200

caccuccgga agcuggugcc cggguucggc aaggccuaca ccaucuucaa caagacccug     1260

auggaggccg acgcccacua caaguccguc cgcacgugga acgagaucau cccgagcaag     1320

gggugccugc gggugggcgg ccgcugccac ccccacguca acgggguguu cuucaacggc     1380

aucauccucg ggcccgacgg caacgugcug auccccgaga ugcaguccag ccugcuccag     1440

cagcacaugg agcugcuggu cuccagcgug aucccgcuca ugcacccccu ggcggacccc     1500

uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg ucgaggugca ccuccccgac     1560

gugcacgagc ggaucagcgg cgucgaccug ggccugccga acugggggaa guacgugcug     1620

cucuccgccg gcgcccugac cgcccugaug cugaucaucu uccucaugac cugcuggcgc     1680

cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg ggaccggccg ggaggucucc     1740

gugaccccgc agagcgggaa gaucaucucc agcugggagu ccuacaagag cggcggcgag     1800

accgggcugu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu     1860

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc     1980

cccccccccc ccccccaaag gcucuuuuca gagccaccag aauu                      2024


<210>  363
<211>  1965
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  363
gggagacgga gacggccugc aggaccugcu cucucagccc ucagccgagg ccuacgccga       60

gccgagugcg cagccgacga ccgggaggag ccgcagccuu caacucugag guacugugau      120

ccgcgcugcc cgccgggccg ccccaguccg cugcugcggc accuccuucc cucgcgcccu      180

cuucgcucgc cagcgccuuc ccugugagcc ugcgucaccg cggccgccaa gcuuaccaug      240

gugccccagg cccuccuguu cgucccgcug cugguguucc cccucugcuu cggcaaguuc      300

cccaucuaca ccauccccga caagcugggg ccguggagcc ccaucgacau ccaccaccug      360

uccugcccca acaaccucgu ggucgaggac gagggcugca ccaaccugag cggguucucc      420

uacauggagc ugaagguggg cuacaucagc gccaucaaga ugaacggguu cacgugcacc      480

ggcgugguca ccgaggcgga gaccuacacg aacuucgugg gcuacgugac caccaccuuc      540

aagcggaagc acuuccgccc cacgccggac gccugccggg ccgccuacaa cuggaagaug      600

gccggggacc cccgcuacga ggagucccuc cacaaccccu accccgacua ccacuggcug      660

cggaccguca agaccaccaa ggagagccug gugaucaucu ccccgagcgu ggcggaccuc      720

gaccccuacg accgcucccu gcacagccgg gucuuccccg gcgggaacug cuccggcgug      780

gccgugagcu ccacguacug cagcaccaac cacgacuaca ccaucuggau gcccgagaac      840

ccgcgccugg ggauguccug cgacaucuuc accaacagcc ggggcaagcg cgccuccaag      900

ggcagcgaga cgugcggguu cgucgacgag cggggccucu acaagucccu gaagggggcc      960

ugcaagcuga agcucugcgg cgugcugggc cugcgccuca uggacgggac cuggguggcg     1020

augcagacca gcaacgagac caaguggugc ccccccggcc agcuggucaa ccugcacgac     1080

uuccggagcg acgagaucga gcaccucgug guggaggagc uggucaagaa gcgcgaggag     1140

ugccuggacg cccucgaguc caucaugacg accaagagcg uguccuuccg gcgccugagc     1200

caccuccgga agcuggugcc cggguucggc aaggccuaca ccaucuucaa caagacccug     1260

auggaggccg acgcccacua caaguccguc cgcacgugga acgagaucau cccgagcaag     1320

gggugccugc gggugggcgg ccgcugccac ccccacguca acgggguguu cuucaacggc     1380

aucauccucg ggcccgacgg caacgugcug auccccgaga ugcaguccag ccugcuccag     1440

cagcacaugg agcugcuggu cuccagcgug aucccgcuca ugcacccccu ggcggacccc     1500

uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg ucgaggugca ccuccccgac     1560

gugcacgagc ggaucagcgg cgucgaccug ggccugccga acugggggaa guacgugcug     1620

cucuccgccg gcgcccugac cgcccugaug cugaucaucu uccucaugac cugcuggcgc     1680

cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg ggaccggccg ggaggucucc     1740

gugaccccgc agagcgggaa gaucaucucc agcugggagu ccuacaagag cggcggcgag     1800

accgggcugu gaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu     1860

ccugccaaau aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     1965


<210>  364
<211>  1031
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  364
gggagacgga gacggccugc aggaccugcu cucucagccc ucagccgagg ccuacgccga       60

gccgagugcg cagccgacga ccgggaggag ccgcagccuu caacucugag guacugugau      120

ccgcgcugcc cgccgggccg ccccaguccg cugcugcggc accuccuucc cucgcgcccu      180

cuucgcucgc cagcgccuuc ccugugagcc ugcgucaccg cggccgccaa gcuuaccaug      240

ggcgugcacg agugccccgc cuggcugugg cuccugcuga gccuccuguc ccugccgcuc      300

gggcugcccg uccugggcgc ccccccgcgg cucaucugcg acagccgcgu gcuggagcgg      360

uaccugcucg aggcgaagga ggccgagaac aucaccaccg ggugcgccga gcacugcucc      420

cugaacgaga acaucacggu gccggacacc aaggucaacu ucuacgccug gaagcgcaug      480

gaggugggcc agcaggccgu ggaggucugg caggggcugg cgcuccugag cgaggccgug      540

cugcggggcc aggcccuccu ggugaacucc agccagcccu gggagccccu gcagcuccac      600

gucgacaagg ccguguccgg ccugcgcagc cugaccaccc uccugcgggc gcugggggcc      660

cagaaggagg ccaucucccc cccggacgcc gccagcgcgg ccccgcuccg cacgaucacc      720

gccgacaccu uccggaagcu guuccgcgug uacuccaacu uccugcgggg caagcucaag      780

cuguacaccg gggaggccug ccgcacgggc gaccggugag gacuaguguc caccuguccc      840

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga      900

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      960

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag     1020

ccaccagaau u                                                          1031


<210>  365
<211>  972
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  365
gggagacgga gacggccugc aggaccugcu cucucagccc ucagccgagg ccuacgccga       60

gccgagugcg cagccgacga ccgggaggag ccgcagccuu caacucugag guacugugau      120

ccgcgcugcc cgccgggccg ccccaguccg cugcugcggc accuccuucc cucgcgcccu      180

cuucgcucgc cagcgccuuc ccugugagcc ugcgucaccg cggccgccaa gcuuaccaug      240

ggcgugcacg agugccccgc cuggcugugg cuccugcuga gccuccuguc ccugccgcuc      300

gggcugcccg uccugggcgc ccccccgcgg cucaucugcg acagccgcgu gcuggagcgg      360

uaccugcucg aggcgaagga ggccgagaac aucaccaccg ggugcgccga gcacugcucc      420

cugaacgaga acaucacggu gccggacacc aaggucaacu ucuacgccug gaagcgcaug      480

gaggugggcc agcaggccgu ggaggucugg caggggcugg cgcuccugag cgaggccgug      540

cugcggggcc aggcccuccu ggugaacucc agccagcccu gggagccccu gcagcuccac      600

gucgacaagg ccguguccgg ccugcgcagc cugaccaccc uccugcgggc gcugggggcc      660

cagaaggagg ccaucucccc cccggacgcc gccagcgcgg ccccgcuccg cacgaucacc      720

gccgacaccu uccggaagcu guuccgcgug uacuccaacu uccugcgggg caagcucaag      780

cuguacaccg gggaggccug ccgcacgggc gaccggugag gacuaguguc caccuguccc      840

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga      900

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      960

aaaaaaagaa uu                                                          972


<210>  366
<211>  2102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  366
gggagacgga gacggccugc aggaccugcu cucucagccc ucagccgagg ccuacgccga       60

gccgagugcg cagccgacga ccgggaggag ccgcagccuu caacucugag guacugugau      120

ccgcgcugcc cgccgggccg ccccaguccg cugcugcggc accuccuucc cucgcgcccu      180

cuucgcucgc cagcgccuuc ccugugagcc ugcgucaccg cggccgccaa gcuuaccaug      240

gaggacgcca agaacaucaa gaagggcccg gcgcccuucu acccgcugga ggacgggacc      300

gccggcgagc agcuccacaa ggccaugaag cgguacgccc uggugccggg cacgaucgcc      360

uucaccgacg cccacaucga ggucgacauc accuacgcgg aguacuucga gaugagcgug      420

cgccuggccg aggccaugaa gcgguacggc cugaacacca accaccggau cguggugugc      480

ucggagaaca gccugcaguu cuucaugccg gugcugggcg cccucuucau cggcguggcc      540

gucgccccgg cgaacgacau cuacaacgag cgggagcugc ugaacagcau ggggaucagc      600

cagccgaccg ugguguucgu gagcaagaag ggccugcaga agauccugaa cgugcagaag      660

aagcugccca ucauccagaa gaucaucauc auggacagca agaccgacua ccagggcuuc      720

cagucgaugu acacguucgu gaccagccac cucccgccgg gcuucaacga guacgacuuc      780

gucccggaga gcuucgaccg ggacaagacc aucgcccuga ucaugaacag cagcggcagc      840

accggccugc cgaagggggu ggcccugccg caccggaccg ccugcgugcg cuucucgcac      900

gcccgggacc ccaucuucgg caaccagauc aucccggaca ccgccauccu gagcguggug      960

ccguuccacc acggcuucgg cauguucacg acccugggcu accucaucug cggcuuccgg     1020

gugguccuga uguaccgguu cgaggaggag cuguuccugc ggagccugca ggacuacaag     1080

auccagagcg cgcugcucgu gccgacccug uucagcuucu ucgccaagag cacccugauc     1140

gacaaguacg accugucgaa ccugcacgag aucgccagcg ggggcgcccc gcugagcaag     1200

gaggugggcg aggccguggc caagcgguuc caccucccgg gcauccgcca gggcuacggc     1260

cugaccgaga ccacgagcgc gauccugauc acccccgagg gggacgacaa gccgggcgcc     1320

gugggcaagg uggucccguu cuucgaggcc aagguggugg accuggacac cggcaagacc     1380

cugggcguga accagcgggg cgagcugugc gugcgggggc cgaugaucau gagcggcuac     1440

gugaacaacc cggaggccac caacgcccuc aucgacaagg acggcuggcu gcacagcggc     1500

gacaucgccu acugggacga ggacgagcac uucuucaucg ucgaccggcu gaagucgcug     1560

aucaaguaca agggcuacca gguggcgccg gccgagcugg agagcauccu gcuccagcac     1620

cccaacaucu ucgacgccgg cguggccggg cugccggacg acgacgccgg cgagcugccg     1680

gccgcggugg uggugcugga gcacggcaag accaugacgg agaaggagau cgucgacuac     1740

guggccagcc aggugaccac cgccaagaag cugcggggcg gcgugguguu cguggacgag     1800

gucccgaagg gccugaccgg gaagcucgac gcccggaaga uccgcgagau ccugaucaag     1860

gccaagaagg gcggcaagau cgccguguga ggacuagugu ccaccugucc cuccugggcu     1920

gcuggauugu cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug     2040

cauccccccc cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa     2100

uu                                                                    2102


<210>  367
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  367
gggagacgga gacggccugc aggaccugcu cucucagccc ucagccgagg ccuacgccga       60

gccgagugcg cagccgacga ccgggaggag ccgcagccuu caacucugag guacugugau      120

ccgcgcugcc cgccgggccg ccccaguccg cugcugcggc accuccuucc cucgcgcccu      180

cuucgcucgc cagcgccuuc ccugugagcc ugcgucaccg cggccgccaa gcuuaccaug      240

gaggacgcca agaacaucaa gaagggcccg gcgcccuucu acccgcugga ggacgggacc      300

gccggcgagc agcuccacaa ggccaugaag cgguacgccc uggugccggg cacgaucgcc      360

uucaccgacg cccacaucga ggucgacauc accuacgcgg aguacuucga gaugagcgug      420

cgccuggccg aggccaugaa gcgguacggc cugaacacca accaccggau cguggugugc      480

ucggagaaca gccugcaguu cuucaugccg gugcugggcg cccucuucau cggcguggcc      540

gucgccccgg cgaacgacau cuacaacgag cgggagcugc ugaacagcau ggggaucagc      600

cagccgaccg ugguguucgu gagcaagaag ggccugcaga agauccugaa cgugcagaag      660

aagcugccca ucauccagaa gaucaucauc auggacagca agaccgacua ccagggcuuc      720

cagucgaugu acacguucgu gaccagccac cucccgccgg gcuucaacga guacgacuuc      780

gucccggaga gcuucgaccg ggacaagacc aucgcccuga ucaugaacag cagcggcagc      840

accggccugc cgaagggggu ggcccugccg caccggaccg ccugcgugcg cuucucgcac      900

gcccgggacc ccaucuucgg caaccagauc aucccggaca ccgccauccu gagcguggug      960

ccguuccacc acggcuucgg cauguucacg acccugggcu accucaucug cggcuuccgg     1020

gugguccuga uguaccgguu cgaggaggag cuguuccugc ggagccugca ggacuacaag     1080

auccagagcg cgcugcucgu gccgacccug uucagcuucu ucgccaagag cacccugauc     1140

gacaaguacg accugucgaa ccugcacgag aucgccagcg ggggcgcccc gcugagcaag     1200

gaggugggcg aggccguggc caagcgguuc caccucccgg gcauccgcca gggcuacggc     1260

cugaccgaga ccacgagcgc gauccugauc acccccgagg gggacgacaa gccgggcgcc     1320

gugggcaagg uggucccguu cuucgaggcc aagguggugg accuggacac cggcaagacc     1380

cugggcguga accagcgggg cgagcugugc gugcgggggc cgaugaucau gagcggcuac     1440

gugaacaacc cggaggccac caacgcccuc aucgacaagg acggcuggcu gcacagcggc     1500

gacaucgccu acugggacga ggacgagcac uucuucaucg ucgaccggcu gaagucgcug     1560

aucaaguaca agggcuacca gguggcgccg gccgagcugg agagcauccu gcuccagcac     1620

cccaacaucu ucgacgccgg cguggccggg cugccggacg acgacgccgg cgagcugccg     1680

gccgcggugg uggugcugga gcacggcaag accaugacgg agaaggagau cgucgacuac     1740

guggccagcc aggugaccac cgccaagaag cugcggggcg gcgugguguu cguggacgag     1800

gucccgaagg gccugaccgg gaagcucgac gcccggaaga uccgcgagau ccugaucaag     1860

gccaagaagg gcggcaagau cgccguguga ggacuagugu ccaccugucc cuccugggcu     1920

gcuggauugu cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     2040

auu                                                                   2043


<210>  368
<211>  2111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  368
gggagacgga gacggccugc aggaccugcu cucucagccc ucagccgagg ccuacgccga       60

gccgagugcg cagccgacga ccgggaggag ccgcagccuu caacucugag guacugugau      120

ccgcgcugcc cgccgggccg ccccaguccg cugcugcggc accuccuucc cucgcgcccu      180

cuucgcucgc cagcgccuuc ccugugagcc ugcgucaccg cggccgccaa gcuuaccaug      240

gagcugcuca uccugaaggc caacgccauc accaccaucc ugacggcggu gaccuucugc      300

uucgccagcg gccagaacau caccgaggag uucuaccagu ccacgugcag cgccgucucc      360

aagggguacc ucagcgcccu gcggaccggc ugguacaccu ccgugaucac caucgagcug      420

agcaacauca aggagaacaa gugcaacggg accgacgcca aggugaagcu caucaagcag      480

gagcuggaca aguacaagaa cgcggucacg gagcugcagc uccugaugca guccaccccg      540

gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca ugaacuacac ccucaacaac      600

gccaagaaga cgaacgugac ccugagcaag aagcgcaagc ggcgcuuccu gggcuuccuc      660

cugggggugg gcuccgccau cgcgagcggc gucgccgugu gcaaggugcu gcaccucgag      720

ggggagguca acaagaucaa gagcgcccug cuguccacca acaaggccgu ggugagccuc      780

uccaacggcg ucagcgugcu gaccuucaag gugcuggacc ucaagaacua caucgacaag      840

cagcugcugc ccauccugaa caagcagagc ugcuccauca gcaacaucga gacggugauc      900

gaguuccagc agaagaacaa ccggcuccug gagaucaccc gcgaguucag cgugaacgcc      960

ggggucacca cccccguguc cacguacaug cugaccaaca gcgagcuccu gucccugauc     1020

aacgacaugc cgaucaccaa cgaccagaag aagcucauga gcaacaacgu gcagaucguc     1080

cggcagcagu ccuacagcau caugugcauc aucaaggagg aggugcuggc guacgugguc     1140

cagcugcccc ucuacggcgu gaucgacacc cccugcugga agcugcacac gagcccccug     1200

ugcaccacca acaccaagga gggguccaac aucugccuca cgcgcaccga ccggggcugg     1260

uacugcgaca acgccggcag cgucuccuuc uucccgcagg ccgagaccug caaggugcag     1320

agcaaccgcg uguucugcga caccaugaac ucccugacgc ugcccagcga gguaaaccuc     1380

ugcaacgucg acaucuucaa ccccaaguac gacugcaaga ucaugaccuc caagaccgac     1440

gugagcucca gcgugaucac cucccugggg gccaucguca gcugcuacgg caagacgaag     1500

ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu ucuccaacgg cugcgacuac     1560

gugagcaaca agggcgugga caccgugucc gucgggaaca cccuguacua cgugaacaag     1620

caggagggca agagccucua cgugaagggg gagcccauca ucaacuucua cgacccgcug     1680

gucuuccccu ccgacgaguu cgacgcgagc aucucccagg ugaacgagaa gaucaaccag     1740

agccuggccu ucauccgcaa guccgacgag cugcuccaca acgugaacgc cggcaagagc     1800

acgaccaaca ucaugaucac caccaucauc aucgucauca ucgugauccu gcugucccuc     1860

aucgccgugg ggcugcugcu cuacugcaag gcccggugag gacuaguguc caccuguccc     1920

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     1980

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2040

aaaaaaaugc aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag     2100

ccaccagaau u                                                          2111


<210>  369
<211>  1957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  369
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggugc       60

cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc aaguucccca      120

ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac caccuguccu      180

gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg uucuccuaca      240

uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg ugcaccggcg      300

uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc accuucaagc      360

ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg aagauggccg      420

gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac uggcugcgga      480

ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg gaccucgacc      540

ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc ggcguggccg      600

ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc gagaacccgc      660

gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc uccaagggca      720

gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag ggggccugca      780

agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg guggcgaugc      840

agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug cacgacuucc      900

ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc gaggagugcc      960

uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc cugagccacc     1020

uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag acccugaugg     1080

aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg agcaaggggu     1140

gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc aacggcauca     1200

uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug cuccagcagc     1260

acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg gaccccucca     1320

ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc cccgacgugc     1380

acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac gugcugcucu     1440

ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc uggcgccggg     1500

ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag gucuccguga     1560

ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc ggcgagaccg     1620

ggcugugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1680

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1740

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     1800

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1920

ccccccccca aaggcucuuu ucagagccac cagaauu                              1957


<210>  370
<211>  1961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  370
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gugccccagg cccuccuguu cgucccgcug cugguguucc cccucugcuu cggcaaguuc      120

cccaucuaca ccauccccga caagcugggg ccguggagcc ccaucgacau ccaccaccug      180

uccugcccca acaaccucgu ggucgaggac gagggcugca ccaaccugag cggguucucc      240

uacauggagc ugaagguggg cuacaucagc gccaucaaga ugaacggguu cacgugcacc      300

ggcgugguca ccgaggcgga gaccuacacg aacuucgugg gcuacgugac caccaccuuc      360

aagcggaagc acuuccgccc cacgccggac gccugccggg ccgccuacaa cuggaagaug      420

gccggggacc cccgcuacga ggagucccuc cacaaccccu accccgacua ccacuggcug      480

cggaccguca agaccaccaa ggagagccug gugaucaucu ccccgagcgu ggcggaccuc      540

gaccccuacg accgcucccu gcacagccgg gucuuccccg gcgggaacug cuccggcgug      600

gccgugagcu ccacguacug cagcaccaac cacgacuaca ccaucuggau gcccgagaac      660

ccgcgccugg ggauguccug cgacaucuuc accaacagcc ggggcaagcg cgccuccaag      720

ggcagcgaga cgugcggguu cgucgacgag cggggccucu acaagucccu gaagggggcc      780

ugcaagcuga agcucugcgg cgugcugggc cugcgccuca uggacgggac cuggguggcg      840

augcagacca gcaacgagac caaguggugc ccccccggcc agcuggucaa ccugcacgac      900

uuccggagcg acgagaucga gcaccucgug guggaggagc uggucaagaa gcgcgaggag      960

ugccuggacg cccucgaguc caucaugacg accaagagcg uguccuuccg gcgccugagc     1020

caccuccgga agcuggugcc cggguucggc aaggccuaca ccaucuucaa caagacccug     1080

auggaggccg acgcccacua caaguccguc cgcacgugga acgagaucau cccgagcaag     1140

gggugccugc gggugggcgg ccgcugccac ccccacguca acgggguguu cuucaacggc     1200

aucauccucg ggcccgacgg caacgugcug auccccgaga ugcaguccag ccugcuccag     1260

cagcacaugg agcugcuggu cuccagcgug aucccgcuca ugcacccccu ggcggacccc     1320

uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg ucgaggugca ccuccccgac     1380

gugcacgagc ggaucagcgg cgucgaccug ggccugccga acugggggaa guacgugcug     1440

cucuccgccg gcgcccugac cgcccugaug cugaucaucu uccucaugac cugcuggcgc     1500

cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg ggaccggccg ggaggucucc     1560

gugaccccgc agagcgggaa gaucaucucc agcugggagu ccuacaagag cggcggcgag     1620

accgggcugu gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa     1680

gagaaagaaa augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag     1740

ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu     1800

ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc     1920

cccccccccc cccaaaggcu cuuuucagag ccaccagaau u                         1961


<210>  371
<211>  1898
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  371
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggugc       60

cccaggcccu ccuguucguc ccgcugcugg uguucccccu cugcuucggc aaguucccca      120

ucuacaccau ccccgacaag cuggggccgu ggagccccau cgacauccac caccuguccu      180

gccccaacaa ccucgugguc gaggacgagg gcugcaccaa ccugagcggg uucuccuaca      240

uggagcugaa ggugggcuac aucagcgcca ucaagaugaa cggguucacg ugcaccggcg      300

uggucaccga ggcggagacc uacacgaacu ucgugggcua cgugaccacc accuucaagc      360

ggaagcacuu ccgccccacg ccggacgccu gccgggccgc cuacaacugg aagauggccg      420

gggacccccg cuacgaggag ucccuccaca accccuaccc cgacuaccac uggcugcgga      480

ccgucaagac caccaaggag agccugguga ucaucucccc gagcguggcg gaccucgacc      540

ccuacgaccg cucccugcac agccgggucu uccccggcgg gaacugcucc ggcguggccg      600

ugagcuccac guacugcagc accaaccacg acuacaccau cuggaugccc gagaacccgc      660

gccuggggau guccugcgac aucuucacca acagccgggg caagcgcgcc uccaagggca      720

gcgagacgug cggguucguc gacgagcggg gccucuacaa gucccugaag ggggccugca      780

agcugaagcu cugcggcgug cugggccugc gccucaugga cgggaccugg guggcgaugc      840

agaccagcaa cgagaccaag uggugccccc ccggccagcu ggucaaccug cacgacuucc      900

ggagcgacga gaucgagcac cucguggugg aggagcuggu caagaagcgc gaggagugcc      960

uggacgcccu cgaguccauc augacgacca agagcguguc cuuccggcgc cugagccacc     1020

uccggaagcu ggugcccggg uucggcaagg ccuacaccau cuucaacaag acccugaugg     1080

aggccgacgc ccacuacaag uccguccgca cguggaacga gaucaucccg agcaaggggu     1140

gccugcgggu gggcggccgc ugccaccccc acgucaacgg gguguucuuc aacggcauca     1200

uccucgggcc cgacggcaac gugcugaucc ccgagaugca guccagccug cuccagcagc     1260

acauggagcu gcuggucucc agcgugaucc cgcucaugca cccccuggcg gaccccucca     1320

ccguguucaa gaacggggac gaggccgagg acuucgucga ggugcaccuc cccgacgugc     1380

acgagcggau cagcggcguc gaccugggcc ugccgaacug ggggaaguac gugcugcucu     1440

ccgccggcgc ccugaccgcc cugaugcuga ucaucuuccu caugaccugc uggcgccggg     1500

ugaaccggag cgagcccacg cagcacaacc ugcgcgggac cggccgggag gucuccguga     1560

ccccgcagag cgggaagauc aucuccagcu gggaguccua caagagcggc ggcgagaccg     1620

ggcugugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1680

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1740

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     1800

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                             1898


<210>  372
<211>  1902
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RAV-G variant

<400>  372
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gugccccagg cccuccuguu cgucccgcug cugguguucc cccucugcuu cggcaaguuc      120

cccaucuaca ccauccccga caagcugggg ccguggagcc ccaucgacau ccaccaccug      180

uccugcccca acaaccucgu ggucgaggac gagggcugca ccaaccugag cggguucucc      240

uacauggagc ugaagguggg cuacaucagc gccaucaaga ugaacggguu cacgugcacc      300

ggcgugguca ccgaggcgga gaccuacacg aacuucgugg gcuacgugac caccaccuuc      360

aagcggaagc acuuccgccc cacgccggac gccugccggg ccgccuacaa cuggaagaug      420

gccggggacc cccgcuacga ggagucccuc cacaaccccu accccgacua ccacuggcug      480

cggaccguca agaccaccaa ggagagccug gugaucaucu ccccgagcgu ggcggaccuc      540

gaccccuacg accgcucccu gcacagccgg gucuuccccg gcgggaacug cuccggcgug      600

gccgugagcu ccacguacug cagcaccaac cacgacuaca ccaucuggau gcccgagaac      660

ccgcgccugg ggauguccug cgacaucuuc accaacagcc ggggcaagcg cgccuccaag      720

ggcagcgaga cgugcggguu cgucgacgag cggggccucu acaagucccu gaagggggcc      780

ugcaagcuga agcucugcgg cgugcugggc cugcgccuca uggacgggac cuggguggcg      840

augcagacca gcaacgagac caaguggugc ccccccggcc agcuggucaa ccugcacgac      900

uuccggagcg acgagaucga gcaccucgug guggaggagc uggucaagaa gcgcgaggag      960

ugccuggacg cccucgaguc caucaugacg accaagagcg uguccuuccg gcgccugagc     1020

caccuccgga agcuggugcc cggguucggc aaggccuaca ccaucuucaa caagacccug     1080

auggaggccg acgcccacua caaguccguc cgcacgugga acgagaucau cccgagcaag     1140

gggugccugc gggugggcgg ccgcugccac ccccacguca acgggguguu cuucaacggc     1200

aucauccucg ggcccgacgg caacgugcug auccccgaga ugcaguccag ccugcuccag     1260

cagcacaugg agcugcuggu cuccagcgug aucccgcuca ugcacccccu ggcggacccc     1320

uccaccgugu ucaagaacgg ggacgaggcc gaggacuucg ucgaggugca ccuccccgac     1380

gugcacgagc ggaucagcgg cgucgaccug ggccugccga acugggggaa guacgugcug     1440

cucuccgccg gcgcccugac cgcccugaug cugaucaucu uccucaugac cugcuggcgc     1500

cgggugaacc ggagcgagcc cacgcagcac aaccugcgcg ggaccggccg ggaggucucc     1560

gugaccccgc agagcgggaa gaucaucucc agcugggagu ccuacaagag cggcggcgag     1620

accgggcugu gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa     1680

gagaaagaaa augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag     1740

ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu     1800

ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa     1860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                        1902


<210>  373
<211>  968
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  373
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

ggcgugcacg agugccccgc cuggcugugg cuccugcuga gccuccuguc ccugccgcuc      120

gggcugcccg uccugggcgc ccccccgcgg cucaucugcg acagccgcgu gcuggagcgg      180

uaccugcucg aggcgaagga ggccgagaac aucaccaccg ggugcgccga gcacugcucc      240

cugaacgaga acaucacggu gccggacacc aaggucaacu ucuacgccug gaagcgcaug      300

gaggugggcc agcaggccgu ggaggucugg caggggcugg cgcuccugag cgaggccgug      360

cugcggggcc aggcccuccu ggugaacucc agccagcccu gggagccccu gcagcuccac      420

gucgacaagg ccguguccgg ccugcgcagc cugaccaccc uccugcgggc gcugggggcc      480

cagaaggagg ccaucucccc cccggacgcc gccagcgcgg ccccgcuccg cacgaucacc      540

gccgacaccu uccggaagcu guuccgcgug uacuccaacu uccugcgggg caagcucaag      600

cuguacaccg gggaggccug ccgcacgggc gaccggugag gacuagugca ucacauuuaa      660

aagcaucuca gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau      720

cucuuuuucu uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu      780

aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca      960

ccagaauu                                                               968


<210>  374
<211>  909
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HsEPO variant

<400>  374
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

ggcgugcacg agugccccgc cuggcugugg cuccugcuga gccuccuguc ccugccgcuc      120

gggcugcccg uccugggcgc ccccccgcgg cucaucugcg acagccgcgu gcuggagcgg      180

uaccugcucg aggcgaagga ggccgagaac aucaccaccg ggugcgccga gcacugcucc      240

cugaacgaga acaucacggu gccggacacc aaggucaacu ucuacgccug gaagcgcaug      300

gaggugggcc agcaggccgu ggaggucugg caggggcugg cgcuccugag cgaggccgug      360

cugcggggcc aggcccuccu ggugaacucc agccagcccu gggagccccu gcagcuccac      420

gucgacaagg ccguguccgg ccugcgcagc cugaccaccc uccugcgggc gcugggggcc      480

cagaaggagg ccaucucccc cccggacgcc gccagcgcgg ccccgcuccg cacgaucacc      540

gccgacaccu uccggaagcu guuccgcgug uacuccaacu uccugcgggg caagcucaag      600

cuguacaccg gggaggccug ccgcacgggc gaccggugag gacuagugca ucacauuuaa      660

aagcaucuca gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau      720

cucuuuuucu uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu      780

aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaagaauu                                                              909


<210>  375
<211>  2039
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  375
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gaggacgcca agaacaucaa gaagggcccg gcgcccuucu acccgcugga ggacgggacc      120

gccggcgagc agcuccacaa ggccaugaag cgguacgccc uggugccggg cacgaucgcc      180

uucaccgacg cccacaucga ggucgacauc accuacgcgg aguacuucga gaugagcgug      240

cgccuggccg aggccaugaa gcgguacggc cugaacacca accaccggau cguggugugc      300

ucggagaaca gccugcaguu cuucaugccg gugcugggcg cccucuucau cggcguggcc      360

gucgccccgg cgaacgacau cuacaacgag cgggagcugc ugaacagcau ggggaucagc      420

cagccgaccg ugguguucgu gagcaagaag ggccugcaga agauccugaa cgugcagaag      480

aagcugccca ucauccagaa gaucaucauc auggacagca agaccgacua ccagggcuuc      540

cagucgaugu acacguucgu gaccagccac cucccgccgg gcuucaacga guacgacuuc      600

gucccggaga gcuucgaccg ggacaagacc aucgcccuga ucaugaacag cagcggcagc      660

accggccugc cgaagggggu ggcccugccg caccggaccg ccugcgugcg cuucucgcac      720

gcccgggacc ccaucuucgg caaccagauc aucccggaca ccgccauccu gagcguggug      780

ccguuccacc acggcuucgg cauguucacg acccugggcu accucaucug cggcuuccgg      840

gugguccuga uguaccgguu cgaggaggag cuguuccugc ggagccugca ggacuacaag      900

auccagagcg cgcugcucgu gccgacccug uucagcuucu ucgccaagag cacccugauc      960

gacaaguacg accugucgaa ccugcacgag aucgccagcg ggggcgcccc gcugagcaag     1020

gaggugggcg aggccguggc caagcgguuc caccucccgg gcauccgcca gggcuacggc     1080

cugaccgaga ccacgagcgc gauccugauc acccccgagg gggacgacaa gccgggcgcc     1140

gugggcaagg uggucccguu cuucgaggcc aagguggugg accuggacac cggcaagacc     1200

cugggcguga accagcgggg cgagcugugc gugcgggggc cgaugaucau gagcggcuac     1260

gugaacaacc cggaggccac caacgcccuc aucgacaagg acggcuggcu gcacagcggc     1320

gacaucgccu acugggacga ggacgagcac uucuucaucg ucgaccggcu gaagucgcug     1380

aucaaguaca agggcuacca gguggcgccg gccgagcugg agagcauccu gcuccagcac     1440

cccaacaucu ucgacgccgg cguggccggg cugccggacg acgacgccgg cgagcugccg     1500

gccgcggugg uggugcugga gcacggcaag accaugacgg agaaggagau cgucgacuac     1560

guggccagcc aggugaccac cgccaagaag cugcggggcg gcgugguguu cguggacgag     1620

gucccgaagg gccugaccgg gaagcucgac gcccggaaga uccgcgagau ccugaucaag     1680

gccaagaagg gcggcaagau cgccguguga ggacuagugc aucacauuua aaagcaucuc     1740

agccuaccau gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc     1800

uuuuucguug guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu     1860

gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau     1980

cccccccccc cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu      2039


<210>  376
<211>  1980
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PpLuc variant

<400>  376
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gaggacgcca agaacaucaa gaagggcccg gcgcccuucu acccgcugga ggacgggacc      120

gccggcgagc agcuccacaa ggccaugaag cgguacgccc uggugccggg cacgaucgcc      180

uucaccgacg cccacaucga ggucgacauc accuacgcgg aguacuucga gaugagcgug      240

cgccuggccg aggccaugaa gcgguacggc cugaacacca accaccggau cguggugugc      300

ucggagaaca gccugcaguu cuucaugccg gugcugggcg cccucuucau cggcguggcc      360

gucgccccgg cgaacgacau cuacaacgag cgggagcugc ugaacagcau ggggaucagc      420

cagccgaccg ugguguucgu gagcaagaag ggccugcaga agauccugaa cgugcagaag      480

aagcugccca ucauccagaa gaucaucauc auggacagca agaccgacua ccagggcuuc      540

cagucgaugu acacguucgu gaccagccac cucccgccgg gcuucaacga guacgacuuc      600

gucccggaga gcuucgaccg ggacaagacc aucgcccuga ucaugaacag cagcggcagc      660

accggccugc cgaagggggu ggcccugccg caccggaccg ccugcgugcg cuucucgcac      720

gcccgggacc ccaucuucgg caaccagauc aucccggaca ccgccauccu gagcguggug      780

ccguuccacc acggcuucgg cauguucacg acccugggcu accucaucug cggcuuccgg      840

gugguccuga uguaccgguu cgaggaggag cuguuccugc ggagccugca ggacuacaag      900

auccagagcg cgcugcucgu gccgacccug uucagcuucu ucgccaagag cacccugauc      960

gacaaguacg accugucgaa ccugcacgag aucgccagcg ggggcgcccc gcugagcaag     1020

gaggugggcg aggccguggc caagcgguuc caccucccgg gcauccgcca gggcuacggc     1080

cugaccgaga ccacgagcgc gauccugauc acccccgagg gggacgacaa gccgggcgcc     1140

gugggcaagg uggucccguu cuucgaggcc aagguggugg accuggacac cggcaagacc     1200

cugggcguga accagcgggg cgagcugugc gugcgggggc cgaugaucau gagcggcuac     1260

gugaacaacc cggaggccac caacgcccuc aucgacaagg acggcuggcu gcacagcggc     1320

gacaucgccu acugggacga ggacgagcac uucuucaucg ucgaccggcu gaagucgcug     1380

aucaaguaca agggcuacca gguggcgccg gccgagcugg agagcauccu gcuccagcac     1440

cccaacaucu ucgacgccgg cguggccggg cugccggacg acgacgccgg cgagcugccg     1500

gccgcggugg uggugcugga gcacggcaag accaugacgg agaaggagau cgucgacuac     1560

guggccagcc aggugaccac cgccaagaag cugcggggcg gcgugguguu cguggacgag     1620

gucccgaagg gccugaccgg gaagcucgac gcccggaaga uccgcgagau ccugaucaag     1680

gccaagaagg gcggcaagau cgccguguga ggacuagugc aucacauuua aaagcaucuc     1740

agccuaccau gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc     1800

uuuuucguug guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu     1860

gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     1980


<210>  377
<211>  2048
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HRSV variant

<400>  377
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gagcugcuca uccugaaggc caacgccauc accaccaucc ugacggcggu gaccuucugc      120

uucgccagcg gccagaacau caccgaggag uucuaccagu ccacgugcag cgccgucucc      180

aagggguacc ucagcgcccu gcggaccggc ugguacaccu ccgugaucac caucgagcug      240

agcaacauca aggagaacaa gugcaacggg accgacgcca aggugaagcu caucaagcag      300

gagcuggaca aguacaagaa cgcggucacg gagcugcagc uccugaugca guccaccccg      360

gcaaccaaca accgcgcccg gcgcgagcug ccccgguuca ugaacuacac ccucaacaac      420

gccaagaaga cgaacgugac ccugagcaag aagcgcaagc ggcgcuuccu gggcuuccuc      480

cugggggugg gcuccgccau cgcgagcggc gucgccgugu gcaaggugcu gcaccucgag      540

ggggagguca acaagaucaa gagcgcccug cuguccacca acaaggccgu ggugagccuc      600

uccaacggcg ucagcgugcu gaccuucaag gugcuggacc ucaagaacua caucgacaag      660

cagcugcugc ccauccugaa caagcagagc ugcuccauca gcaacaucga gacggugauc      720

gaguuccagc agaagaacaa ccggcuccug gagaucaccc gcgaguucag cgugaacgcc      780

ggggucacca cccccguguc cacguacaug cugaccaaca gcgagcuccu gucccugauc      840

aacgacaugc cgaucaccaa cgaccagaag aagcucauga gcaacaacgu gcagaucguc      900

cggcagcagu ccuacagcau caugugcauc aucaaggagg aggugcuggc guacgugguc      960

cagcugcccc ucuacggcgu gaucgacacc cccugcugga agcugcacac gagcccccug     1020

ugcaccacca acaccaagga gggguccaac aucugccuca cgcgcaccga ccggggcugg     1080

uacugcgaca acgccggcag cgucuccuuc uucccgcagg ccgagaccug caaggugcag     1140

agcaaccgcg uguucugcga caccaugaac ucccugacgc ugcccagcga gguaaaccuc     1200

ugcaacgucg acaucuucaa ccccaaguac gacugcaaga ucaugaccuc caagaccgac     1260

gugagcucca gcgugaucac cucccugggg gccaucguca gcugcuacgg caagacgaag     1320

ugcaccgcca gcaacaagaa ccgggggauc aucaagaccu ucuccaacgg cugcgacuac     1380

gugagcaaca agggcgugga caccgugucc gucgggaaca cccuguacua cgugaacaag     1440

caggagggca agagccucua cgugaagggg gagcccauca ucaacuucua cgacccgcug     1500

gucuuccccu ccgacgaguu cgacgcgagc aucucccagg ugaacgagaa gaucaaccag     1560

agccuggccu ucauccgcaa guccgacgag cugcuccaca acgugaacgc cggcaagagc     1620

acgaccaaca ucaugaucac caccaucauc aucgucauca ucgugauccu gcugucccuc     1680

aucgccgugg ggcugcugcu cuacugcaag gcccggugag gacuagugca ucacauuuaa     1740

aagcaucuca gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau     1800

cucuuuuucu uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu     1860

aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaugcauc cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca     2040

ccagaauu                                                              2048


<210>  378
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide tag

<400>  378

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  379
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide tag

<400>  379

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  380
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide tag

<400>  380

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  381
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS

<400>  381

Pro Lys Lys Lys Arg Arg Val 
1               5           


<210>  382
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS

<400>  382

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  383
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  383

Gly Gly Gly Gly Ser 
1               5   


<210>  384
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa can be amino acid Arg or Lys

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa can be amino acid Arg or Lys

<400>  384

Lys Xaa Xaa Xaa 
1               


<210>  385
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_HSD17B4

<400>  385
gtcccgcagt cggcgtccag cggctctgct tgttcgtgtg tgtgtcgttg caggccttat       60

tc                                                                      62


<210>  386
<211>  62
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_HSD17B4

<400>  386
gucccgcagu cggcguccag cggcucugcu uguucgugug ugugucguug caggccuuau       60

uc                                                                      62


<210>  387
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_RPL32

<400>  387
ggcgctgcct acggaggtgg cagccatctc cttctcggca tc                          42


<210>  388
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_RPL32

<400>  388
ggcgcugccu acggaggugg cagccaucuc cuucucggca uc                          42


<210>  389
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_RPL31.1

<400>  389
cccgtgaccc ggaagttgta cggctacgcg actttccctc ccacaaaccc tcgcgccctt       60

cctttcctac ttgggcccgg caga                                              84


<210>  390
<211>  84
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_RPL31.1

<400>  390
cccgugaccc ggaaguugua cggcuacgcg acuuucccuc ccacaaaccc ucgcgcccuu       60

ccuuuccuac uugggcccgg caga                                              84


<210>  391
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Ndufa4.1

<400>  391
gtccgctcag ccaggttgca gaagcggctt agcgtgtgtc ctaatcttct ctctgcgtgt       60

aggtaggcct gtgccgcaaa c                                                 81


<210>  392
<211>  81
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Ndufa4.1

<400>  392
guccgcucag ccagguugca gaagcggcuu agcguguguc cuaaucuucu cucugcgugu       60

agguaggccu gugccgcaaa c                                                 81


<210>  393
<211>  159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Slc7a3.1

<400>  393
gggcgcttgg cttgcaagga ccctgagctg cggcattgaa gcacacccaa cccaactcga       60

ctgaagtcag cctcactgaa ccggatctga gaatcttctc tctctgggct tgccagggct      120

ctccgaacct agctagcatc ctcttcaatt ccaactaga                             159


<210>  394
<211>  159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Slc7a3.1

<400>  394
gggcgcuugg cuugcaagga cccugagcug cggcauugaa gcacacccaa cccaacucga       60

cugaagucag ccucacugaa ccggaucuga gaaucuucuc ucucugggcu ugccagggcu      120

cuccgaaccu agcuagcauc cucuucaauu ccaacuaga                             159


<210>  395
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Nosip.1

<400>  395
ctcctgtcgg gcggaagtag gaggagtaga gtttaaaaac agtactcttt ttccggttcg       60

ggacgtagtt gaagcaacga caagccggat aaccgctctt gagacagg                   108


<210>  396
<211>  108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_Nosip.1

<400>  396
cuccugucgg gcggaaguag gaggaguaga guuuaaaaac aguacucuuu uuccgguucg       60

ggacguaguu gaagcaacga caagccggau aaccgcucuu gagacagg                   108


<210>  397
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_ATP5A1

<400>  397
gcggctcggc cattttgtcc cagtcagtcc ggaggctgcg gctgcagaag taccgcctgc       60

ggagtaactg caaag                                                        75


<210>  398
<211>  75
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR_ATP5A1

<400>  398
gcggcucggc cauuuugucc cagucagucc ggaggcugcg gcugcagaag uaccgccugc       60

ggaguaacug caaag                                                        75


<210>  399
<211>  353
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_Gnas.1

<400>  399
gaagggaaca cccaaattta attcagcctt aagcacaatt aattaagagt gaaacgtaat       60

tgtacaagca gttggtcacc caccataggg catgatcaac accgcaacct ttcctttttc      120

ccccagtgat tctgaaaaac ccctcttccc ttcagcttgc ttagatgttc caaatttagt      180

aagcttaagg cggcctacag aagaaaaaga aaaaaaaggc cacaaaagtt ccctctcact      240

ttcagtaaat aaaataaaag cagcaacaga aataaagaaa taaatgaaat tcaaaatgaa      300

ataaatattg tgttgtgcag cattaaaaaa tcaataaaaa ttaaaaatga gca             353


<210>  400
<211>  353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_Gnas.1

<400>  400
gaagggaaca cccaaauuua auucagccuu aagcacaauu aauuaagagu gaaacguaau       60

uguacaagca guuggucacc caccauaggg caugaucaac accgcaaccu uuccuuuuuc      120

ccccagugau ucugaaaaac cccucuuccc uucagcuugc uuagauguuc caaauuuagu      180

aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu cccucucacu      240

uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau ucaaaaugaa      300

auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga gca             353


<210>  401
<211>  121
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_CASP1.1

<400>  401
aataaggaaa ctgtatgaat gtctgtgggc aggaagtgaa gagatccttc tgtaaaggtt       60

tttggaatta tgtctgctga ataataaact tttttgaaat aataaatctg gtagaaaaat      120

g                                                                      121


<210>  402
<211>  121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_CASP1.1

<400>  402
aauaaggaaa cuguaugaau gucugugggc aggaagugaa gagauccuuc uguaaagguu       60

uuuggaauua ugucugcuga auaauaaacu uuuuugaaau aauaaaucug guagaaaaau      120

g                                                                      121


<210>  403
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_PSMB3.1

<400>  403
ccctgttccc agagcccact tttttttctt tttttgaaat aaaatagcct gtctttc          57


<210>  404
<211>  57
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_PSMB3.1

<400>  404
cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuuc          57


<210>  405
<211>  187
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_ALB7.1

<400>  405
gcatcacatt taaaagcatc tcagcctacc atgagaataa gagaaagaaa atgaagatca       60

atagcttatt catctctttt tctttttcgt tggtgtaaag ccaacaccct gtctaaaaaa      120

cataaatttc tttaatcatt ttgcctcttt tctctgtgct tcaattaata aaaaatggaa      180

agaacct                                                                187


<210>  406
<211>  187
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_ALB7.1

<400>  406
gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa augaagauca       60

auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa      120

cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa      180

agaaccu                                                                187


<210>  407
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_RPS9.1

<400>  407
gtccacctgt ccctcctggg ctgctggatt gtctcgtttt cctgccaaat aaacaggatc       60

agcgctttac                                                              70


<210>  408
<211>  70
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR_RPS9.1

<400>  408
guccaccugu cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc       60

agcgcuuuac                                                              70


<210>  409
<211>  51
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS2 variant

<400>  409
auggccccaa agaagaagcg gaaggucggu auccacggag ucccagcagc c                51


<210>  410
<211>  48
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS4 variant

<400>  410
aagcguccug cugcuacuaa gaaagcuggu caagcuaaga aaaagaaa                    48


<210>  411
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  411
auggacaaga aguacagcau cggccuggac aucggcacca acucuguggg cugggccgug       60

aucaccgacg aguacaaggu gcccagcaag aaauucaagg ugcugggcaa caccgaccgg      120

cacagcauca agaagaaccu gaucggagcc cugcuguucg acagcggcga aacagccgag      180

gccacccggc ugaagagaac cgccagaaga agauacacca gacggaagaa ccggaucugc      240

uaucugcaag agaucuucag caacgagaug gccaaggugg acgacagcuu cuuccacaga      300

cuggaagagu ccuuccuggu ggaagaggau aagaagcacg agcggcaccc caucuucggc      360

aacaucgugg acgagguggc cuaccacgag aaguacccca ccaucuacca ccugagaaag      420

aaacuggugg acagcaccga caaggccgac cugcggcuga ucuaucuggc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggcgacc ugaaccccga caacagcgac      540

guggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggaaaacccc      600

aucaacgcca gcggcgugga cgccaaggcc auccugucug ccagacugag caagagcaga      660

cggcuggaaa aucugaucgc ccagcugccc ggcgagaaga agaauggccu guucggcaac      720

cugauugccc ugagccuggg ccugaccccc aacuucaaga gcaacuucga ccuggccgag      780

gaugccaaac ugcagcugag caaggacacc uacgacgacg accuggacaa ccugcuggcc      840

cagaucggcg accaguacgc cgaccuguuu cuggccgcca agaaccuguc cgacgccauc      900

cugcugagcg acauccugag agugaacacc gagaucacca aggccccccu gagcgccucu      960

augaucaaga gauacgacga gcaccaccag gaccugaccc ugcugaaagc ucucgugcgg     1020

cagcagcugc cugagaagua caaagagauu uucuucgacc agagcaagaa cggcuacgcc     1080

ggcuacauug acggcggagc cagccaggaa gaguucuaca aguucaucaa gcccauccug     1140

gaaaagaugg acggcaccga ggaacugcuc gugaagcuga acagagagga ccugcugcgg     1200

aagcagcgga ccuucgacaa cggcagcauc ccccaccaga uccaccuggg agagcugcac     1260

gccauucugc ggcggcagga agauuuuuac ccauuccuga aggacaaccg ggaaaagauc     1320

gagaagaucc ugaccuuccg cauccccuac uacgugggcc cucuggccag gggaaacagc     1380

agauucgccu ggaugaccag aaagagcgag gaaaccauca cccccuggaa cuucgaggaa     1440

gugguggaca agggcgcuuc cgcccagagc uucaucgagc ggaugaccaa cuucgauaag     1500

aaccugccca acgagaaggu gcugcccaag cacagccugc uguacgagua cuucaccgug     1560

uauaacgagc ugaccaaagu gaaauacgug accgagggaa ugagaaagcc cgccuuccug     1620

agcggcgagc agaaaaaggc caucguggac cugcuguuca agaccaaccg gaaagugacc     1680

gugaagcagc ugaaagagga cuacuucaag aaaaucgagu gcuucgacuc cguggaaauc     1740

uccggcgugg aagaucgguu caacgccucc cugggcacau accacgaucu gcugaaaauu     1800

aucaaggaca aggacuuccu ggacaaugag gaaaacgagg acauucugga agauaucgug     1860

cugacccuga cacuguuuga ggacagagag augaucgagg aacggcugaa aaccuaugcc     1920

caccuguucg acgacaaagu gaugaagcag cugaagcggc ggagauacac cggcuggggc     1980

aggcugagcc ggaagcugau caacggcauc cgggacaagc aguccggcaa gacaauccug     2040

gauuuccuga aguccgacgg cuucgccaac agaaacuuca ugcagcugau ccacgacgac     2100

agccugaccu uuaaagagga cauccagaaa gcccaggugu ccggccaggg cgauagccug     2160

cacgagcaca uugccaaucu ggccggcagc cccgccauua agaagggcau ccugcagaca     2220

gugaaggugg uggacgagcu cgugaaagug augggccggc acaagcccga gaacaucgug     2280

aucgaaaugg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga     2340

augaagcgga ucgaagaggg caucaaagag cugggcagcc agauccugaa agaacacccc     2400

guggaaaaca cccagcugca gaacgagaag cuguaccugu acuaccugca gaaugggcgg     2460

gauauguacg uggaccagga acuggacauc aaccggcugu ccgacuacga uguggaccau     2520

aucgugccuc agagcuuucu gaaggacgac uccaucgaca acaaggugcu gaccagaagc     2580

gacaagaacc ggggcaagag cgacaacgug cccuccgaag aggucgugaa gaagaugaag     2640

aacuacuggc ggcagcugcu gaacgccaag cugauuaccc agagaaaguu cgacaaucug     2700

accaaggccg agagaggcgg ccugagcgaa cuggauaagg ccggcuucau caagagacag     2760

cugguggaaa cccggcagau cacaaagcac guggcacaga uccuggacuc ccggaugaac     2820

acuaaguacg acgagaauga caagcugauc cgggaaguga aagugaucac ccugaagucc     2880

aagcuggugu ccgauuuccg gaaggauuuc caguuuuaca aagugcgcga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gccgucgugg gaaccgcccu gaucaaaaag     3000

uacccuaagc uggaaagcga guucguguac ggcgacuaca agguguacga cgugcggaag     3060

augaucgcca agagcgagca ggaaaucggc aaggcuaccg ccaaguacuu cuucuacagc     3120

aacaucauga acuuuuucaa gaccgagauu acccuggcca acggcgagau ccggaagcgg     3180

ccucugaucg agacaaacgg cgaaaccggg gagaucgugu gggauaaggg ccgggauuuu     3240

gccaccgugc ggaaagugcu gagcaugccc caagugaaua ucgugaaaaa gaccgaggug     3300

cagacaggcg gcuucagcaa agagucuauc cugcccaaga ggaacagcga uaagcugauc     3360

gccagaaaga aggacuggga cccuaagaag uacggcggcu ucgacagccc caccguggcc     3420

uauucugugc uggugguggc caaaguggaa aagggcaagu ccaagaaacu gaagagugug     3480

aaagagcugc uggggaucac caucauggaa agaagcagcu ucgagaagaa ucccaucgac     3540

uuucuggaag ccaagggcua caaagaagug aaaaaggacc ugaucaucaa gcugccuaag     3600

uacucccugu ucgagcugga aaacggccgg aagagaaugc uggccucugc cggcgaacug     3660

cagaagggaa acgaacuggc ccugcccucc aaauauguga acuuccugua ccuggccagc     3720

cacuaugaga agcugaaggg cucccccgag gauaaugagc agaaacagcu guuuguggaa     3780

cagcacaagc acuaccugga cgagaucauc gagcagauca gcgaguucuc caagagagug     3840

auccuggccg acgcuaaucu ggacaaagug cuguccgccu acaacaagca ccgggauaag     3900

cccaucagag agcaggccga gaauaucauc caccuguuua cccugaccaa ucugggagcc     3960

ccugccgccu ucaaguacuu ugacaccacc aucgaccgga agagguacac cagcaccaaa     4020

gaggugcugg acgccacccu gauccaccag agcaucaccg gccuguacga gacacggauc     4080

gaccugucuc agcugggagg cgacuaa                                         4107


<210>  412
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  412
auggccccaa agaagaagcg gaaggucggu auccacggag ucccagcagc cgacaagaag       60

uacagcaucg gccuggacau cggcaccaac ucugugggcu gggccgugau caccgacgag      120

uacaaggugc ccagcaagaa auucaaggug cugggcaaca ccgaccggca cagcaucaag      180

aagaaccuga ucggagcccu gcuguucgac agcggcgaaa cagccgaggc cacccggcug      240

aagagaaccg ccagaagaag auacaccaga cggaagaacc ggaucugcua ucugcaagag      300

aucuucagca acgagauggc caagguggac gacagcuucu uccacagacu ggaagagucc      360

uuccuggugg aagaggauaa gaagcacgag cggcacccca ucuucggcaa caucguggac      420

gagguggccu accacgagaa guaccccacc aucuaccacc ugagaaagaa acugguggac      480

agcaccgaca aggccgaccu gcggcugauc uaucuggccc uggcccacau gaucaaguuc      540

cggggccacu uccugaucga gggcgaccug aaccccgaca acagcgacgu ggacaagcug      600

uucauccagc uggugcagac cuacaaccag cuguucgagg aaaaccccau caacgccagc      660

ggcguggacg ccaaggccau ccugucugcc agacugagca agagcagacg gcuggaaaau      720

cugaucgccc agcugcccgg cgagaagaag aauggccugu ucggcaaccu gauugcccug      780

agccugggcc ugacccccaa cuucaagagc aacuucgacc uggccgagga ugccaaacug      840

cagcugagca aggacaccua cgacgacgac cuggacaacc ugcuggccca gaucggcgac      900

caguacgccg accuguuucu ggccgccaag aaccuguccg acgccauccu gcugagcgac      960

auccugagag ugaacaccga gaucaccaag gccccccuga gcgccucuau gaucaagaga     1020

uacgacgagc accaccagga ccugacccug cugaaagcuc ucgugcggca gcagcugccu     1080

gagaaguaca aagagauuuu cuucgaccag agcaagaacg gcuacgccgg cuacauugac     1140

ggcggagcca gccaggaaga guucuacaag uucaucaagc ccauccugga aaagauggac     1200

ggcaccgagg aacugcucgu gaagcugaac agagaggacc ugcugcggaa gcagcggacc     1260

uucgacaacg gcagcauccc ccaccagauc caccugggag agcugcacgc cauucugcgg     1320

cggcaggaag auuuuuaccc auuccugaag gacaaccggg aaaagaucga gaagauccug     1380

accuuccgca uccccuacua cgugggcccu cuggccaggg gaaacagcag auucgccugg     1440

augaccagaa agagcgagga aaccaucacc cccuggaacu ucgaggaagu gguggacaag     1500

ggcgcuuccg cccagagcuu caucgagcgg augaccaacu ucgauaagaa ccugcccaac     1560

gagaaggugc ugcccaagca cagccugcug uacgaguacu ucaccgugua uaacgagcug     1620

accaaaguga aauacgugac cgagggaaug agaaagcccg ccuuccugag cggcgagcag     1680

aaaaaggcca ucguggaccu gcuguucaag accaaccgga aagugaccgu gaagcagcug     1740

aaagaggacu acuucaagaa aaucgagugc uucgacuccg uggaaaucuc cggcguggaa     1800

gaucgguuca acgccucccu gggcacauac cacgaucugc ugaaaauuau caaggacaag     1860

gacuuccugg acaaugagga aaacgaggac auucuggaag auaucgugcu gacccugaca     1920

cuguuugagg acagagagau gaucgaggaa cggcugaaaa ccuaugccca ccuguucgac     1980

gacaaaguga ugaagcagcu gaagcggcgg agauacaccg gcuggggcag gcugagccgg     2040

aagcugauca acggcauccg ggacaagcag uccggcaaga caauccugga uuuccugaag     2100

uccgacggcu ucgccaacag aaacuucaug cagcugaucc acgacgacag ccugaccuuu     2160

aaagaggaca uccagaaagc ccaggugucc ggccagggcg auagccugca cgagcacauu     2220

gccaaucugg ccggcagccc cgccauuaag aagggcaucc ugcagacagu gaagguggug     2280

gacgagcucg ugaaagugau gggccggcac aagcccgaga acaucgugau cgaaauggcc     2340

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaau gaagcggauc     2400

gaagagggca ucaaagagcu gggcagccag auccugaaag aacaccccgu ggaaaacacc     2460

cagcugcaga acgagaagcu guaccuguac uaccugcaga augggcggga uauguacgug     2520

gaccaggaac uggacaucaa ccggcugucc gacuacgaug uggaccauau cgugccucag     2580

agcuuucuga aggacgacuc caucgacaac aaggugcuga ccagaagcga caagaaccgg     2640

ggcaagagcg acaacgugcc cuccgaagag gucgugaaga agaugaagaa cuacuggcgg     2700

cagcugcuga acgccaagcu gauuacccag agaaaguucg acaaucugac caaggccgag     2760

agaggcggcc ugagcgaacu ggauaaggcc ggcuucauca agagacagcu gguggaaacc     2820

cggcagauca caaagcacgu ggcacagauc cuggacuccc ggaugaacac uaaguacgac     2880

gagaaugaca agcugauccg ggaagugaaa gugaucaccc ugaaguccaa gcuggugucc     2940

gauuuccgga aggauuucca guuuuacaaa gugcgcgaga ucaacaacua ccaccacgcc     3000

cacgacgccu accugaacgc cgucguggga accgcccuga ucaaaaagua cccuaagcug     3060

gaaagcgagu ucguguacgg cgacuacaag guguacgacg ugcggaagau gaucgccaag     3120

agcgagcagg aaaucggcaa ggcuaccgcc aaguacuucu ucuacagcaa caucaugaac     3180

uuuuucaaga ccgagauuac ccuggccaac ggcgagaucc ggaagcggcc ucugaucgag     3240

acaaacggcg aaaccgggga gaucgugugg gauaagggcc gggauuuugc caccgugcgg     3300

aaagugcuga gcaugcccca agugaauauc gugaaaaaga ccgaggugca gacaggcggc     3360

uucagcaaag agucuauccu gcccaagagg aacagcgaua agcugaucgc cagaaagaag     3420

gacugggacc cuaagaagua cggcggcuuc gacagcccca ccguggccua uucugugcug     3480

gugguggcca aaguggaaaa gggcaagucc aagaaacuga agagugugaa agagcugcug     3540

gggaucacca ucauggaaag aagcagcuuc gagaagaauc ccaucgacuu ucuggaagcc     3600

aagggcuaca aagaagugaa aaaggaccug aucaucaagc ugccuaagua cucccuguuc     3660

gagcuggaaa acggccggaa gagaaugcug gccucugccg gcgaacugca gaagggaaac     3720

gaacuggccc ugcccuccaa auaugugaac uuccuguacc uggccagcca cuaugagaag     3780

cugaagggcu cccccgagga uaaugagcag aaacagcugu uuguggaaca gcacaagcac     3840

uaccuggacg agaucaucga gcagaucagc gaguucucca agagagugau ccuggccgac     3900

gcuaaucugg acaaagugcu guccgccuac aacaagcacc gggauaagcc caucagagag     3960

caggccgaga auaucaucca ccuguuuacc cugaccaauc ugggagcccc ugccgccuuc     4020

aaguacuuug acaccaccau cgaccggaag agguacacca gcaccaaaga ggugcuggac     4080

gccacccuga uccaccagag caucaccggc cuguacgaga cacggaucga ccugucucag     4140

cugggaggcg acaagcgucc ugcugcuacu aagaaagcug gucaagcuaa gaaaaagaaa     4200

uaa                                                                   4203


<210>  413
<211>  4716
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  413
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gccccaaaga agaagcggaa ggucgguauc cacggagucc      120

cagcagccga caagaaguac agcaucggcc uggacaucgg caccaacucu gugggcuggg      180

ccgugaucac cgacgaguac aaggugccca gcaagaaauu caaggugcug ggcaacaccg      240

accggcacag caucaagaag aaccugaucg gagcccugcu guucgacagc ggcgaaacag      300

ccgaggccac ccggcugaag agaaccgcca gaagaagaua caccagacgg aagaaccgga      360

ucugcuaucu gcaagagauc uucagcaacg agauggccaa gguggacgac agcuucuucc      420

acagacugga agaguccuuc cugguggaag aggauaagaa gcacgagcgg caccccaucu      480

ucggcaacau cguggacgag guggccuacc acgagaagua ccccaccauc uaccaccuga      540

gaaagaaacu gguggacagc accgacaagg ccgaccugcg gcugaucuau cuggcccugg      600

cccacaugau caaguuccgg ggccacuucc ugaucgaggg cgaccugaac cccgacaaca      660

gcgacgugga caagcuguuc auccagcugg ugcagaccua caaccagcug uucgaggaaa      720

accccaucaa cgccagcggc guggacgcca aggccauccu gucugccaga cugagcaaga      780

gcagacggcu ggaaaaucug aucgcccagc ugcccggcga gaagaagaau ggccuguucg      840

gcaaccugau ugcccugagc cugggccuga cccccaacuu caagagcaac uucgaccugg      900

ccgaggaugc caaacugcag cugagcaagg acaccuacga cgacgaccug gacaaccugc      960

uggcccagau cggcgaccag uacgccgacc uguuucuggc cgccaagaac cuguccgacg     1020

ccauccugcu gagcgacauc cugagaguga acaccgagau caccaaggcc ccccugagcg     1080

ccucuaugau caagagauac gacgagcacc accaggaccu gacccugcug aaagcucucg     1140

ugcggcagca gcugccugag aaguacaaag agauuuucuu cgaccagagc aagaacggcu     1200

acgccggcua cauugacggc ggagccagcc aggaagaguu cuacaaguuc aucaagccca     1260

uccuggaaaa gauggacggc accgaggaac ugcucgugaa gcugaacaga gaggaccugc     1320

ugcggaagca gcggaccuuc gacaacggca gcauccccca ccagauccac cugggagagc     1380

ugcacgccau ucugcggcgg caggaagauu uuuacccauu ccugaaggac aaccgggaaa     1440

agaucgagaa gauccugacc uuccgcaucc ccuacuacgu gggcccucug gccaggggaa     1500

acagcagauu cgccuggaug accagaaaga gcgaggaaac caucaccccc uggaacuucg     1560

aggaaguggu ggacaagggc gcuuccgccc agagcuucau cgagcggaug accaacuucg     1620

auaagaaccu gcccaacgag aaggugcugc ccaagcacag ccugcuguac gaguacuuca     1680

ccguguauaa cgagcugacc aaagugaaau acgugaccga gggaaugaga aagcccgccu     1740

uccugagcgg cgagcagaaa aaggccaucg uggaccugcu guucaagacc aaccggaaag     1800

ugaccgugaa gcagcugaaa gaggacuacu ucaagaaaau cgagugcuuc gacuccgugg     1860

aaaucuccgg cguggaagau cgguucaacg ccucccuggg cacauaccac gaucugcuga     1920

aaauuaucaa ggacaaggac uuccuggaca augaggaaaa cgaggacauu cuggaagaua     1980

ucgugcugac ccugacacug uuugaggaca gagagaugau cgaggaacgg cugaaaaccu     2040

augcccaccu guucgacgac aaagugauga agcagcugaa gcggcggaga uacaccggcu     2100

ggggcaggcu gagccggaag cugaucaacg gcauccggga caagcagucc ggcaagacaa     2160

uccuggauuu ccugaagucc gacggcuucg ccaacagaaa cuucaugcag cugauccacg     2220

acgacagccu gaccuuuaaa gaggacaucc agaaagccca gguguccggc cagggcgaua     2280

gccugcacga gcacauugcc aaucuggccg gcagccccgc cauuaagaag ggcauccugc     2340

agacagugaa ggugguggac gagcucguga aagugauggg ccggcacaag cccgagaaca     2400

ucgugaucga aauggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg     2460

agagaaugaa gcggaucgaa gagggcauca aagagcuggg cagccagauc cugaaagaac     2520

accccgugga aaacacccag cugcagaacg agaagcugua ccuguacuac cugcagaaug     2580

ggcgggauau guacguggac caggaacugg acaucaaccg gcuguccgac uacgaugugg     2640

accauaucgu gccucagagc uuucugaagg acgacuccau cgacaacaag gugcugacca     2700

gaagcgacaa gaaccggggc aagagcgaca acgugcccuc cgaagagguc gugaagaaga     2760

ugaagaacua cuggcggcag cugcugaacg ccaagcugau uacccagaga aaguucgaca     2820

aucugaccaa ggccgagaga ggcggccuga gcgaacugga uaaggccggc uucaucaaga     2880

gacagcuggu ggaaacccgg cagaucacaa agcacguggc acagauccug gacucccgga     2940

ugaacacuaa guacgacgag aaugacaagc ugauccggga agugaaagug aucacccuga     3000

aguccaagcu gguguccgau uuccggaagg auuuccaguu uuacaaagug cgcgagauca     3060

acaacuacca ccacgcccac gacgccuacc ugaacgccgu cgugggaacc gcccugauca     3120

aaaaguaccc uaagcuggaa agcgaguucg uguacggcga cuacaaggug uacgacgugc     3180

ggaagaugau cgccaagagc gagcaggaaa ucggcaaggc uaccgccaag uacuucuucu     3240

acagcaacau caugaacuuu uucaagaccg agauuacccu ggccaacggc gagauccgga     3300

agcggccucu gaucgagaca aacggcgaaa ccggggagau cgugugggau aagggccggg     3360

auuuugccac cgugcggaaa gugcugagca ugccccaagu gaauaucgug aaaaagaccg     3420

aggugcagac aggcggcuuc agcaaagagu cuauccugcc caagaggaac agcgauaagc     3480

ugaucgccag aaagaaggac ugggacccua agaaguacgg cggcuucgac agccccaccg     3540

uggccuauuc ugugcuggug guggccaaag uggaaaaggg caaguccaag aaacugaaga     3600

gugugaaaga gcugcugggg aucaccauca uggaaagaag cagcuucgag aagaauccca     3660

ucgacuuucu ggaagccaag ggcuacaaag aagugaaaaa ggaccugauc aucaagcugc     3720

cuaaguacuc ccuguucgag cuggaaaacg gccggaagag aaugcuggcc ucugccggcg     3780

aacugcagaa gggaaacgaa cuggcccugc ccuccaaaua ugugaacuuc cuguaccugg     3840

ccagccacua ugagaagcug aagggcuccc ccgaggauaa ugagcagaaa cagcuguuug     3900

uggaacagca caagcacuac cuggacgaga ucaucgagca gaucagcgag uucuccaaga     3960

gagugauccu ggccgacgcu aaucuggaca aagugcuguc cgccuacaac aagcaccggg     4020

auaagcccau cagagagcag gccgagaaua ucauccaccu guuuacccug accaaucugg     4080

gagccccugc cgccuucaag uacuuugaca ccaccaucga ccggaagagg uacaccagca     4140

ccaaagaggu gcuggacgcc acccugaucc accagagcau caccggccug uacgagacac     4200

ggaucgaccu gucucagcug ggaggcgaca agcguccugc ugcuacuaag aaagcugguc     4260

aagcuaagaa aaagaaauaa ggacuaguga agggaacacc caaauuuaau ucagccuuaa     4320

gcacaauuaa uuaagaguga aacguaauug uacaagcagu uggucaccca ccauagggca     4380

ugaucaacac cgcaaccuuu ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu     4440

cagcuugcuu agauguucca aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa     4500

aaaaaggcca caaaaguucc cucucacuuu caguaaauaa aauaaaagca gcaacagaaa     4560

uaaagaaaua aaugaaauuc aaaaugaaau aaauauugug uugugcagca uuaaaaaauc     4620

aauaaaaauu aaaaaugagc aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               4716


<210>  414
<211>  4813
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  414
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

ccaaagaaga agcggaaggu cgguauccac ggagucccag cagccgacaa gaaguacagc      240

aucggccugg acaucggcac caacucugug ggcugggccg ugaucaccga cgaguacaag      300

gugcccagca agaaauucaa ggugcugggc aacaccgacc ggcacagcau caagaagaac      360

cugaucggag cccugcuguu cgacagcggc gaaacagccg aggccacccg gcugaagaga      420

accgccagaa gaagauacac cagacggaag aaccggaucu gcuaucugca agagaucuuc      480

agcaacgaga uggccaaggu ggacgacagc uucuuccaca gacuggaaga guccuuccug      540

guggaagagg auaagaagca cgagcggcac cccaucuucg gcaacaucgu ggacgaggug      600

gccuaccacg agaaguaccc caccaucuac caccugagaa agaaacuggu ggacagcacc      660

gacaaggccg accugcggcu gaucuaucug gcccuggccc acaugaucaa guuccggggc      720

cacuuccuga ucgagggcga ccugaacccc gacaacagcg acguggacaa gcuguucauc      780

cagcuggugc agaccuacaa ccagcuguuc gaggaaaacc ccaucaacgc cagcggcgug      840

gacgccaagg ccauccuguc ugccagacug agcaagagca gacggcugga aaaucugauc      900

gcccagcugc ccggcgagaa gaagaauggc cuguucggca accugauugc ccugagccug      960

ggccugaccc ccaacuucaa gagcaacuuc gaccuggccg aggaugccaa acugcagcug     1020

agcaaggaca ccuacgacga cgaccuggac aaccugcugg cccagaucgg cgaccaguac     1080

gccgaccugu uucuggccgc caagaaccug uccgacgcca uccugcugag cgacauccug     1140

agagugaaca ccgagaucac caaggccccc cugagcgccu cuaugaucaa gagauacgac     1200

gagcaccacc aggaccugac ccugcugaaa gcucucgugc ggcagcagcu gccugagaag     1260

uacaaagaga uuuucuucga ccagagcaag aacggcuacg ccggcuacau ugacggcgga     1320

gccagccagg aagaguucua caaguucauc aagcccaucc uggaaaagau ggacggcacc     1380

gaggaacugc ucgugaagcu gaacagagag gaccugcugc ggaagcagcg gaccuucgac     1440

aacggcagca ucccccacca gauccaccug ggagagcugc acgccauucu gcggcggcag     1500

gaagauuuuu acccauuccu gaaggacaac cgggaaaaga ucgagaagau ccugaccuuc     1560

cgcauccccu acuacguggg cccucuggcc aggggaaaca gcagauucgc cuggaugacc     1620

agaaagagcg aggaaaccau cacccccugg aacuucgagg aaguggugga caagggcgcu     1680

uccgcccaga gcuucaucga gcggaugacc aacuucgaua agaaccugcc caacgagaag     1740

gugcugccca agcacagccu gcuguacgag uacuucaccg uguauaacga gcugaccaaa     1800

gugaaauacg ugaccgaggg aaugagaaag cccgccuucc ugagcggcga gcagaaaaag     1860

gccaucgugg accugcuguu caagaccaac cggaaaguga ccgugaagca gcugaaagag     1920

gacuacuuca agaaaaucga gugcuucgac uccguggaaa ucuccggcgu ggaagaucgg     1980

uucaacgccu cccugggcac auaccacgau cugcugaaaa uuaucaagga caaggacuuc     2040

cuggacaaug aggaaaacga ggacauucug gaagauaucg ugcugacccu gacacuguuu     2100

gaggacagag agaugaucga ggaacggcug aaaaccuaug cccaccuguu cgacgacaaa     2160

gugaugaagc agcugaagcg gcggagauac accggcuggg gcaggcugag ccggaagcug     2220

aucaacggca uccgggacaa gcaguccggc aagacaaucc uggauuuccu gaaguccgac     2280

ggcuucgcca acagaaacuu caugcagcug auccacgacg acagccugac cuuuaaagag     2340

gacauccaga aagcccaggu guccggccag ggcgauagcc ugcacgagca cauugccaau     2400

cuggccggca gccccgccau uaagaagggc auccugcaga cagugaaggu gguggacgag     2460

cucgugaaag ugaugggccg gcacaagccc gagaacaucg ugaucgaaau ggccagagag     2520

aaccagacca cccagaaggg acagaagaac agccgcgaga gaaugaagcg gaucgaagag     2580

ggcaucaaag agcugggcag ccagauccug aaagaacacc ccguggaaaa cacccagcug     2640

cagaacgaga agcuguaccu guacuaccug cagaaugggc gggauaugua cguggaccag     2700

gaacuggaca ucaaccggcu guccgacuac gauguggacc auaucgugcc ucagagcuuu     2760

cugaaggacg acuccaucga caacaaggug cugaccagaa gcgacaagaa ccggggcaag     2820

agcgacaacg ugcccuccga agaggucgug aagaagauga agaacuacug gcggcagcug     2880

cugaacgcca agcugauuac ccagagaaag uucgacaauc ugaccaaggc cgagagaggc     2940

ggccugagcg aacuggauaa ggccggcuuc aucaagagac agcuggugga aacccggcag     3000

aucacaaagc acguggcaca gauccuggac ucccggauga acacuaagua cgacgagaau     3060

gacaagcuga uccgggaagu gaaagugauc acccugaagu ccaagcuggu guccgauuuc     3120

cggaaggauu uccaguuuua caaagugcgc gagaucaaca acuaccacca cgcccacgac     3180

gccuaccuga acgccgucgu gggaaccgcc cugaucaaaa aguacccuaa gcuggaaagc     3240

gaguucgugu acggcgacua caagguguac gacgugcgga agaugaucgc caagagcgag     3300

caggaaaucg gcaaggcuac cgccaaguac uucuucuaca gcaacaucau gaacuuuuuc     3360

aagaccgaga uuacccuggc caacggcgag auccggaagc ggccucugau cgagacaaac     3420

ggcgaaaccg gggagaucgu gugggauaag ggccgggauu uugccaccgu gcggaaagug     3480

cugagcaugc cccaagugaa uaucgugaaa aagaccgagg ugcagacagg cggcuucagc     3540

aaagagucua uccugcccaa gaggaacagc gauaagcuga ucgccagaaa gaaggacugg     3600

gacccuaaga aguacggcgg cuucgacagc cccaccgugg ccuauucugu gcugguggug     3660

gccaaagugg aaaagggcaa guccaagaaa cugaagagug ugaaagagcu gcuggggauc     3720

accaucaugg aaagaagcag cuucgagaag aaucccaucg acuuucugga agccaagggc     3780

uacaaagaag ugaaaaagga ccugaucauc aagcugccua aguacucccu guucgagcug     3840

gaaaacggcc ggaagagaau gcuggccucu gccggcgaac ugcagaaggg aaacgaacug     3900

gcccugcccu ccaaauaugu gaacuuccug uaccuggcca gccacuauga gaagcugaag     3960

ggcucccccg aggauaauga gcagaaacag cuguuugugg aacagcacaa gcacuaccug     4020

gacgagauca ucgagcagau cagcgaguuc uccaagagag ugauccuggc cgacgcuaau     4080

cuggacaaag ugcuguccgc cuacaacaag caccgggaua agcccaucag agagcaggcc     4140

gagaauauca uccaccuguu uacccugacc aaucugggag ccccugccgc cuucaaguac     4200

uuugacacca ccaucgaccg gaagagguac accagcacca aagaggugcu ggacgccacc     4260

cugauccacc agagcaucac cggccuguac gagacacgga ucgaccuguc ucagcuggga     4320

ggcgacaagc guccugcugc uacuaagaaa gcuggucaag cuaagaaaaa gaaauaagga     4380

cuagugaagg gaacacccaa auuuaauuca gccuuaagca caauuaauua agagugaaac     4440

guaauuguac aagcaguugg ucacccacca uagggcauga ucaacaccgc aaccuuuccu     4500

uuuuccccca gugauucuga aaaaccccuc uucccuucag cuugcuuaga uguuccaaau     4560

uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa aaggccacaa aaguucccuc     4620

ucacuuucag uaaauaaaau aaaagcagca acagaaauaa agaaauaaau gaaauucaaa     4680

augaaauaaa uauuguguug ugcagcauua aaaaaucaau aaaaauuaaa aaugagcaag     4740

aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4800

aaaaaaaaga auu                                                        4813


<210>  415
<211>  4497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  415
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccaa agaagaagcg gaaggucggu      120

auccacggag ucccagcagc cgacaagaag uacagcaucg gccuggacau cggcaccaac      180

ucugugggcu gggccgugau caccgacgag uacaaggugc ccagcaagaa auucaaggug      240

cugggcaaca ccgaccggca cagcaucaag aagaaccuga ucggagcccu gcuguucgac      300

agcggcgaaa cagccgaggc cacccggcug aagagaaccg ccagaagaag auacaccaga      360

cggaagaacc ggaucugcua ucugcaagag aucuucagca acgagauggc caagguggac      420

gacagcuucu uccacagacu ggaagagucc uuccuggugg aagaggauaa gaagcacgag      480

cggcacccca ucuucggcaa caucguggac gagguggccu accacgagaa guaccccacc      540

aucuaccacc ugagaaagaa acugguggac agcaccgaca aggccgaccu gcggcugauc      600

uaucuggccc uggcccacau gaucaaguuc cggggccacu uccugaucga gggcgaccug      660

aaccccgaca acagcgacgu ggacaagcug uucauccagc uggugcagac cuacaaccag      720

cuguucgagg aaaaccccau caacgccagc ggcguggacg ccaaggccau ccugucugcc      780

agacugagca agagcagacg gcuggaaaau cugaucgccc agcugcccgg cgagaagaag      840

aauggccugu ucggcaaccu gauugcccug agccugggcc ugacccccaa cuucaagagc      900

aacuucgacc uggccgagga ugccaaacug cagcugagca aggacaccua cgacgacgac      960

cuggacaacc ugcuggccca gaucggcgac caguacgccg accuguuucu ggccgccaag     1020

aaccuguccg acgccauccu gcugagcgac auccugagag ugaacaccga gaucaccaag     1080

gccccccuga gcgccucuau gaucaagaga uacgacgagc accaccagga ccugacccug     1140

cugaaagcuc ucgugcggca gcagcugccu gagaaguaca aagagauuuu cuucgaccag     1200

agcaagaacg gcuacgccgg cuacauugac ggcggagcca gccaggaaga guucuacaag     1260

uucaucaagc ccauccugga aaagauggac ggcaccgagg aacugcucgu gaagcugaac     1320

agagaggacc ugcugcggaa gcagcggacc uucgacaacg gcagcauccc ccaccagauc     1380

caccugggag agcugcacgc cauucugcgg cggcaggaag auuuuuaccc auuccugaag     1440

gacaaccggg aaaagaucga gaagauccug accuuccgca uccccuacua cgugggcccu     1500

cuggccaggg gaaacagcag auucgccugg augaccagaa agagcgagga aaccaucacc     1560

cccuggaacu ucgaggaagu gguggacaag ggcgcuuccg cccagagcuu caucgagcgg     1620

augaccaacu ucgauaagaa ccugcccaac gagaaggugc ugcccaagca cagccugcug     1680

uacgaguacu ucaccgugua uaacgagcug accaaaguga aauacgugac cgagggaaug     1740

agaaagcccg ccuuccugag cggcgagcag aaaaaggcca ucguggaccu gcuguucaag     1800

accaaccgga aagugaccgu gaagcagcug aaagaggacu acuucaagaa aaucgagugc     1860

uucgacuccg uggaaaucuc cggcguggaa gaucgguuca acgccucccu gggcacauac     1920

cacgaucugc ugaaaauuau caaggacaag gacuuccugg acaaugagga aaacgaggac     1980

auucuggaag auaucgugcu gacccugaca cuguuugagg acagagagau gaucgaggaa     2040

cggcugaaaa ccuaugccca ccuguucgac gacaaaguga ugaagcagcu gaagcggcgg     2100

agauacaccg gcuggggcag gcugagccgg aagcugauca acggcauccg ggacaagcag     2160

uccggcaaga caauccugga uuuccugaag uccgacggcu ucgccaacag aaacuucaug     2220

cagcugaucc acgacgacag ccugaccuuu aaagaggaca uccagaaagc ccaggugucc     2280

ggccagggcg auagccugca cgagcacauu gccaaucugg ccggcagccc cgccauuaag     2340

aagggcaucc ugcagacagu gaagguggug gacgagcucg ugaaagugau gggccggcac     2400

aagcccgaga acaucgugau cgaaauggcc agagagaacc agaccaccca gaagggacag     2460

aagaacagcc gcgagagaau gaagcggauc gaagagggca ucaaagagcu gggcagccag     2520

auccugaaag aacaccccgu ggaaaacacc cagcugcaga acgagaagcu guaccuguac     2580

uaccugcaga augggcggga uauguacgug gaccaggaac uggacaucaa ccggcugucc     2640

gacuacgaug uggaccauau cgugccucag agcuuucuga aggacgacuc caucgacaac     2700

aaggugcuga ccagaagcga caagaaccgg ggcaagagcg acaacgugcc cuccgaagag     2760

gucgugaaga agaugaagaa cuacuggcgg cagcugcuga acgccaagcu gauuacccag     2820

agaaaguucg acaaucugac caaggccgag agaggcggcc ugagcgaacu ggauaaggcc     2880

ggcuucauca agagacagcu gguggaaacc cggcagauca caaagcacgu ggcacagauc     2940

cuggacuccc ggaugaacac uaaguacgac gagaaugaca agcugauccg ggaagugaaa     3000

gugaucaccc ugaaguccaa gcuggugucc gauuuccgga aggauuucca guuuuacaaa     3060

gugcgcgaga ucaacaacua ccaccacgcc cacgacgccu accugaacgc cgucguggga     3120

accgcccuga ucaaaaagua cccuaagcug gaaagcgagu ucguguacgg cgacuacaag     3180

guguacgacg ugcggaagau gaucgccaag agcgagcagg aaaucggcaa ggcuaccgcc     3240

aaguacuucu ucuacagcaa caucaugaac uuuuucaaga ccgagauuac ccuggccaac     3300

ggcgagaucc ggaagcggcc ucugaucgag acaaacggcg aaaccgggga gaucgugugg     3360

gauaagggcc gggauuuugc caccgugcgg aaagugcuga gcaugcccca agugaauauc     3420

gugaaaaaga ccgaggugca gacaggcggc uucagcaaag agucuauccu gcccaagagg     3480

aacagcgaua agcugaucgc cagaaagaag gacugggacc cuaagaagua cggcggcuuc     3540

gacagcccca ccguggccua uucugugcug gugguggcca aaguggaaaa gggcaagucc     3600

aagaaacuga agagugugaa agagcugcug gggaucacca ucauggaaag aagcagcuuc     3660

gagaagaauc ccaucgacuu ucuggaagcc aagggcuaca aagaagugaa aaaggaccug     3720

aucaucaagc ugccuaagua cucccuguuc gagcuggaaa acggccggaa gagaaugcug     3780

gccucugccg gcgaacugca gaagggaaac gaacuggccc ugcccuccaa auaugugaac     3840

uuccuguacc uggccagcca cuaugagaag cugaagggcu cccccgagga uaaugagcag     3900

aaacagcugu uuguggaaca gcacaagcac uaccuggacg agaucaucga gcagaucagc     3960

gaguucucca agagagugau ccuggccgac gcuaaucugg acaaagugcu guccgccuac     4020

aacaagcacc gggauaagcc caucagagag caggccgaga auaucaucca ccuguuuacc     4080

cugaccaauc ugggagcccc ugccgccuuc aaguacuuug acaccaccau cgaccggaag     4140

agguacacca gcaccaaaga ggugcuggac gccacccuga uccaccagag caucaccggc     4200

cuguacgaga cacggaucga ccugucucag cugggaggcg acaagcgucc ugcugcuacu     4260

aagaaagcug gucaagcuaa gaaaaagaaa uaaggacuag uaauaaggaa acuguaugaa     4320

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4380

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4497


<210>  416
<211>  4439
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  416
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ccccaaagaa gaagcggaag      120

gucgguaucc acggaguccc agcagccgac aagaaguaca gcaucggccu ggacaucggc      180

accaacucug ugggcugggc cgugaucacc gacgaguaca aggugcccag caagaaauuc      240

aaggugcugg gcaacaccga ccggcacagc aucaagaaga accugaucgg agcccugcug      300

uucgacagcg gcgaaacagc cgaggccacc cggcugaaga gaaccgccag aagaagauac      360

accagacgga agaaccggau cugcuaucug caagagaucu ucagcaacga gauggccaag      420

guggacgaca gcuucuucca cagacuggaa gaguccuucc ugguggaaga ggauaagaag      480

cacgagcggc accccaucuu cggcaacauc guggacgagg uggccuacca cgagaaguac      540

cccaccaucu accaccugag aaagaaacug guggacagca ccgacaaggc cgaccugcgg      600

cugaucuauc uggcccuggc ccacaugauc aaguuccggg gccacuuccu gaucgagggc      660

gaccugaacc ccgacaacag cgacguggac aagcuguuca uccagcuggu gcagaccuac      720

aaccagcugu ucgaggaaaa ccccaucaac gccagcggcg uggacgccaa ggccauccug      780

ucugccagac ugagcaagag cagacggcug gaaaaucuga ucgcccagcu gcccggcgag      840

aagaagaaug gccuguucgg caaccugauu gcccugagcc ugggccugac ccccaacuuc      900

aagagcaacu ucgaccuggc cgaggaugcc aaacugcagc ugagcaagga caccuacgac      960

gacgaccugg acaaccugcu ggcccagauc ggcgaccagu acgccgaccu guuucuggcc     1020

gccaagaacc uguccgacgc cauccugcug agcgacaucc ugagagugaa caccgagauc     1080

accaaggccc cccugagcgc cucuaugauc aagagauacg acgagcacca ccaggaccug     1140

acccugcuga aagcucucgu gcggcagcag cugccugaga aguacaaaga gauuuucuuc     1200

gaccagagca agaacggcua cgccggcuac auugacggcg gagccagcca ggaagaguuc     1260

uacaaguuca ucaagcccau ccuggaaaag auggacggca ccgaggaacu gcucgugaag     1320

cugaacagag aggaccugcu gcggaagcag cggaccuucg acaacggcag caucccccac     1380

cagauccacc ugggagagcu gcacgccauu cugcggcggc aggaagauuu uuacccauuc     1440

cugaaggaca accgggaaaa gaucgagaag auccugaccu uccgcauccc cuacuacgug     1500

ggcccucugg ccaggggaaa cagcagauuc gccuggauga ccagaaagag cgaggaaacc     1560

aucacccccu ggaacuucga ggaaguggug gacaagggcg cuuccgccca gagcuucauc     1620

gagcggauga ccaacuucga uaagaaccug cccaacgaga aggugcugcc caagcacagc     1680

cugcuguacg aguacuucac cguguauaac gagcugacca aagugaaaua cgugaccgag     1740

ggaaugagaa agcccgccuu ccugagcggc gagcagaaaa aggccaucgu ggaccugcug     1800

uucaagacca accggaaagu gaccgugaag cagcugaaag aggacuacuu caagaaaauc     1860

gagugcuucg acuccgugga aaucuccggc guggaagauc gguucaacgc cucccugggc     1920

acauaccacg aucugcugaa aauuaucaag gacaaggacu uccuggacaa ugaggaaaac     1980

gaggacauuc uggaagauau cgugcugacc cugacacugu uugaggacag agagaugauc     2040

gaggaacggc ugaaaaccua ugcccaccug uucgacgaca aagugaugaa gcagcugaag     2100

cggcggagau acaccggcug gggcaggcug agccggaagc ugaucaacgg cauccgggac     2160

aagcaguccg gcaagacaau ccuggauuuc cugaaguccg acggcuucgc caacagaaac     2220

uucaugcagc ugauccacga cgacagccug accuuuaaag aggacaucca gaaagcccag     2280

guguccggcc agggcgauag ccugcacgag cacauugcca aucuggccgg cagccccgcc     2340

auuaagaagg gcauccugca gacagugaag gugguggacg agcucgugaa agugaugggc     2400

cggcacaagc ccgagaacau cgugaucgaa auggccagag agaaccagac cacccagaag     2460

ggacagaaga acagccgcga gagaaugaag cggaucgaag agggcaucaa agagcugggc     2520

agccagaucc ugaaagaaca ccccguggaa aacacccagc ugcagaacga gaagcuguac     2580

cuguacuacc ugcagaaugg gcgggauaug uacguggacc aggaacugga caucaaccgg     2640

cuguccgacu acgaugugga ccauaucgug ccucagagcu uucugaagga cgacuccauc     2700

gacaacaagg ugcugaccag aagcgacaag aaccggggca agagcgacaa cgugcccucc     2760

gaagaggucg ugaagaagau gaagaacuac uggcggcagc ugcugaacgc caagcugauu     2820

acccagagaa aguucgacaa ucugaccaag gccgagagag gcggccugag cgaacuggau     2880

aaggccggcu ucaucaagag acagcuggug gaaacccggc agaucacaaa gcacguggca     2940

cagauccugg acucccggau gaacacuaag uacgacgaga augacaagcu gauccgggaa     3000

gugaaaguga ucacccugaa guccaagcug guguccgauu uccggaagga uuuccaguuu     3060

uacaaagugc gcgagaucaa caacuaccac cacgcccacg acgccuaccu gaacgccguc     3120

gugggaaccg cccugaucaa aaaguacccu aagcuggaaa gcgaguucgu guacggcgac     3180

uacaaggugu acgacgugcg gaagaugauc gccaagagcg agcaggaaau cggcaaggcu     3240

accgccaagu acuucuucua cagcaacauc augaacuuuu ucaagaccga gauuacccug     3300

gccaacggcg agauccggaa gcggccucug aucgagacaa acggcgaaac cggggagauc     3360

gugugggaua agggccggga uuuugccacc gugcggaaag ugcugagcau gccccaagug     3420

aauaucguga aaaagaccga ggugcagaca ggcggcuuca gcaaagaguc uauccugccc     3480

aagaggaaca gcgauaagcu gaucgccaga aagaaggacu gggacccuaa gaaguacggc     3540

ggcuucgaca gccccaccgu ggccuauucu gugcuggugg uggccaaagu ggaaaagggc     3600

aaguccaaga aacugaagag ugugaaagag cugcugggga ucaccaucau ggaaagaagc     3660

agcuucgaga agaaucccau cgacuuucug gaagccaagg gcuacaaaga agugaaaaag     3720

gaccugauca ucaagcugcc uaaguacucc cuguucgagc uggaaaacgg ccggaagaga     3780

augcuggccu cugccggcga acugcagaag ggaaacgaac uggcccugcc cuccaaauau     3840

gugaacuucc uguaccuggc cagccacuau gagaagcuga agggcucccc cgaggauaau     3900

gagcagaaac agcuguuugu ggaacagcac aagcacuacc uggacgagau caucgagcag     3960

aucagcgagu ucuccaagag agugauccug gccgacgcua aucuggacaa agugcugucc     4020

gccuacaaca agcaccggga uaagcccauc agagagcagg ccgagaauau cauccaccug     4080

uuuacccuga ccaaucuggg agccccugcc gccuucaagu acuuugacac caccaucgac     4140

cggaagaggu acaccagcac caaagaggug cuggacgcca cccugaucca ccagagcauc     4200

accggccugu acgagacacg gaucgaccug ucucagcugg gaggcgacaa gcguccugcu     4260

gcuacuaaga aagcugguca agcuaagaaa aagaaauaag gacuaguccc uguucccaga     4320

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      4439


<210>  417
<211>  4420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  417
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gccccaaaga agaagcggaa ggucgguauc cacggagucc      120

cagcagccga caagaaguac agcaucggcc uggacaucgg caccaacucu gugggcuggg      180

ccgugaucac cgacgaguac aaggugccca gcaagaaauu caaggugcug ggcaacaccg      240

accggcacag caucaagaag aaccugaucg gagcccugcu guucgacagc ggcgaaacag      300

ccgaggccac ccggcugaag agaaccgcca gaagaagaua caccagacgg aagaaccgga      360

ucugcuaucu gcaagagauc uucagcaacg agauggccaa gguggacgac agcuucuucc      420

acagacugga agaguccuuc cugguggaag aggauaagaa gcacgagcgg caccccaucu      480

ucggcaacau cguggacgag guggccuacc acgagaagua ccccaccauc uaccaccuga      540

gaaagaaacu gguggacagc accgacaagg ccgaccugcg gcugaucuau cuggcccugg      600

cccacaugau caaguuccgg ggccacuucc ugaucgaggg cgaccugaac cccgacaaca      660

gcgacgugga caagcuguuc auccagcugg ugcagaccua caaccagcug uucgaggaaa      720

accccaucaa cgccagcggc guggacgcca aggccauccu gucugccaga cugagcaaga      780

gcagacggcu ggaaaaucug aucgcccagc ugcccggcga gaagaagaau ggccuguucg      840

gcaaccugau ugcccugagc cugggccuga cccccaacuu caagagcaac uucgaccugg      900

ccgaggaugc caaacugcag cugagcaagg acaccuacga cgacgaccug gacaaccugc      960

uggcccagau cggcgaccag uacgccgacc uguuucuggc cgccaagaac cuguccgacg     1020

ccauccugcu gagcgacauc cugagaguga acaccgagau caccaaggcc ccccugagcg     1080

ccucuaugau caagagauac gacgagcacc accaggaccu gacccugcug aaagcucucg     1140

ugcggcagca gcugccugag aaguacaaag agauuuucuu cgaccagagc aagaacggcu     1200

acgccggcua cauugacggc ggagccagcc aggaagaguu cuacaaguuc aucaagccca     1260

uccuggaaaa gauggacggc accgaggaac ugcucgugaa gcugaacaga gaggaccugc     1320

ugcggaagca gcggaccuuc gacaacggca gcauccccca ccagauccac cugggagagc     1380

ugcacgccau ucugcggcgg caggaagauu uuuacccauu ccugaaggac aaccgggaaa     1440

agaucgagaa gauccugacc uuccgcaucc ccuacuacgu gggcccucug gccaggggaa     1500

acagcagauu cgccuggaug accagaaaga gcgaggaaac caucaccccc uggaacuucg     1560

aggaaguggu ggacaagggc gcuuccgccc agagcuucau cgagcggaug accaacuucg     1620

auaagaaccu gcccaacgag aaggugcugc ccaagcacag ccugcuguac gaguacuuca     1680

ccguguauaa cgagcugacc aaagugaaau acgugaccga gggaaugaga aagcccgccu     1740

uccugagcgg cgagcagaaa aaggccaucg uggaccugcu guucaagacc aaccggaaag     1800

ugaccgugaa gcagcugaaa gaggacuacu ucaagaaaau cgagugcuuc gacuccgugg     1860

aaaucuccgg cguggaagau cgguucaacg ccucccuggg cacauaccac gaucugcuga     1920

aaauuaucaa ggacaaggac uuccuggaca augaggaaaa cgaggacauu cuggaagaua     1980

ucgugcugac ccugacacug uuugaggaca gagagaugau cgaggaacgg cugaaaaccu     2040

augcccaccu guucgacgac aaagugauga agcagcugaa gcggcggaga uacaccggcu     2100

ggggcaggcu gagccggaag cugaucaacg gcauccggga caagcagucc ggcaagacaa     2160

uccuggauuu ccugaagucc gacggcuucg ccaacagaaa cuucaugcag cugauccacg     2220

acgacagccu gaccuuuaaa gaggacaucc agaaagccca gguguccggc cagggcgaua     2280

gccugcacga gcacauugcc aaucuggccg gcagccccgc cauuaagaag ggcauccugc     2340

agacagugaa ggugguggac gagcucguga aagugauggg ccggcacaag cccgagaaca     2400

ucgugaucga aauggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg     2460

agagaaugaa gcggaucgaa gagggcauca aagagcuggg cagccagauc cugaaagaac     2520

accccgugga aaacacccag cugcagaacg agaagcugua ccuguacuac cugcagaaug     2580

ggcgggauau guacguggac caggaacugg acaucaaccg gcuguccgac uacgaugugg     2640

accauaucgu gccucagagc uuucugaagg acgacuccau cgacaacaag gugcugacca     2700

gaagcgacaa gaaccggggc aagagcgaca acgugcccuc cgaagagguc gugaagaaga     2760

ugaagaacua cuggcggcag cugcugaacg ccaagcugau uacccagaga aaguucgaca     2820

aucugaccaa ggccgagaga ggcggccuga gcgaacugga uaaggccggc uucaucaaga     2880

gacagcuggu ggaaacccgg cagaucacaa agcacguggc acagauccug gacucccgga     2940

ugaacacuaa guacgacgag aaugacaagc ugauccggga agugaaagug aucacccuga     3000

aguccaagcu gguguccgau uuccggaagg auuuccaguu uuacaaagug cgcgagauca     3060

acaacuacca ccacgcccac gacgccuacc ugaacgccgu cgugggaacc gcccugauca     3120

aaaaguaccc uaagcuggaa agcgaguucg uguacggcga cuacaaggug uacgacgugc     3180

ggaagaugau cgccaagagc gagcaggaaa ucggcaaggc uaccgccaag uacuucuucu     3240

acagcaacau caugaacuuu uucaagaccg agauuacccu ggccaacggc gagauccgga     3300

agcggccucu gaucgagaca aacggcgaaa ccggggagau cgugugggau aagggccggg     3360

auuuugccac cgugcggaaa gugcugagca ugccccaagu gaauaucgug aaaaagaccg     3420

aggugcagac aggcggcuuc agcaaagagu cuauccugcc caagaggaac agcgauaagc     3480

ugaucgccag aaagaaggac ugggacccua agaaguacgg cggcuucgac agccccaccg     3540

uggccuauuc ugugcuggug guggccaaag uggaaaaggg caaguccaag aaacugaaga     3600

gugugaaaga gcugcugggg aucaccauca uggaaagaag cagcuucgag aagaauccca     3660

ucgacuuucu ggaagccaag ggcuacaaag aagugaaaaa ggaccugauc aucaagcugc     3720

cuaaguacuc ccuguucgag cuggaaaacg gccggaagag aaugcuggcc ucugccggcg     3780

aacugcagaa gggaaacgaa cuggcccugc ccuccaaaua ugugaacuuc cuguaccugg     3840

ccagccacua ugagaagcug aagggcuccc ccgaggauaa ugagcagaaa cagcuguuug     3900

uggaacagca caagcacuac cuggacgaga ucaucgagca gaucagcgag uucuccaaga     3960

gagugauccu ggccgacgcu aaucuggaca aagugcuguc cgccuacaac aagcaccggg     4020

auaagcccau cagagagcag gccgagaaua ucauccaccu guuuacccug accaaucugg     4080

gagccccugc cgccuucaag uacuuugaca ccaccaucga ccggaagagg uacaccagca     4140

ccaaagaggu gcuggacgcc acccugaucc accagagcau caccggccug uacgagacac     4200

ggaucgaccu gucucagcug ggaggcgaca agcguccugc ugcuacuaag aaagcugguc     4260

aagcuaagaa aaagaaauaa ggacuagucc cuguucccag agcccacuuu uuuuucuuuu     4320

uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                           4420


<210>  418
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  418
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gccccaaaga agaagcggaa ggucgguauc cacggagucc cagcagccga caagaaguac      120

agcaucggcc uggacaucgg caccaacucu gugggcuggg ccgugaucac cgacgaguac      180

aaggugccca gcaagaaauu caaggugcug ggcaacaccg accggcacag caucaagaag      240

aaccugaucg gagcccugcu guucgacagc ggcgaaacag ccgaggccac ccggcugaag      300

agaaccgcca gaagaagaua caccagacgg aagaaccgga ucugcuaucu gcaagagauc      360

uucagcaacg agauggccaa gguggacgac agcuucuucc acagacugga agaguccuuc      420

cugguggaag aggauaagaa gcacgagcgg caccccaucu ucggcaacau cguggacgag      480

guggccuacc acgagaagua ccccaccauc uaccaccuga gaaagaaacu gguggacagc      540

accgacaagg ccgaccugcg gcugaucuau cuggcccugg cccacaugau caaguuccgg      600

ggccacuucc ugaucgaggg cgaccugaac cccgacaaca gcgacgugga caagcuguuc      660

auccagcugg ugcagaccua caaccagcug uucgaggaaa accccaucaa cgccagcggc      720

guggacgcca aggccauccu gucugccaga cugagcaaga gcagacggcu ggaaaaucug      780

aucgcccagc ugcccggcga gaagaagaau ggccuguucg gcaaccugau ugcccugagc      840

cugggccuga cccccaacuu caagagcaac uucgaccugg ccgaggaugc caaacugcag      900

cugagcaagg acaccuacga cgacgaccug gacaaccugc uggcccagau cggcgaccag      960

uacgccgacc uguuucuggc cgccaagaac cuguccgacg ccauccugcu gagcgacauc     1020

cugagaguga acaccgagau caccaaggcc ccccugagcg ccucuaugau caagagauac     1080

gacgagcacc accaggaccu gacccugcug aaagcucucg ugcggcagca gcugccugag     1140

aaguacaaag agauuuucuu cgaccagagc aagaacggcu acgccggcua cauugacggc     1200

ggagccagcc aggaagaguu cuacaaguuc aucaagccca uccuggaaaa gauggacggc     1260

accgaggaac ugcucgugaa gcugaacaga gaggaccugc ugcggaagca gcggaccuuc     1320

gacaacggca gcauccccca ccagauccac cugggagagc ugcacgccau ucugcggcgg     1380

caggaagauu uuuacccauu ccugaaggac aaccgggaaa agaucgagaa gauccugacc     1440

uuccgcaucc ccuacuacgu gggcccucug gccaggggaa acagcagauu cgccuggaug     1500

accagaaaga gcgaggaaac caucaccccc uggaacuucg aggaaguggu ggacaagggc     1560

gcuuccgccc agagcuucau cgagcggaug accaacuucg auaagaaccu gcccaacgag     1620

aaggugcugc ccaagcacag ccugcuguac gaguacuuca ccguguauaa cgagcugacc     1680

aaagugaaau acgugaccga gggaaugaga aagcccgccu uccugagcgg cgagcagaaa     1740

aaggccaucg uggaccugcu guucaagacc aaccggaaag ugaccgugaa gcagcugaaa     1800

gaggacuacu ucaagaaaau cgagugcuuc gacuccgugg aaaucuccgg cguggaagau     1860

cgguucaacg ccucccuggg cacauaccac gaucugcuga aaauuaucaa ggacaaggac     1920

uuccuggaca augaggaaaa cgaggacauu cuggaagaua ucgugcugac ccugacacug     1980

uuugaggaca gagagaugau cgaggaacgg cugaaaaccu augcccaccu guucgacgac     2040

aaagugauga agcagcugaa gcggcggaga uacaccggcu ggggcaggcu gagccggaag     2100

cugaucaacg gcauccggga caagcagucc ggcaagacaa uccuggauuu ccugaagucc     2160

gacggcuucg ccaacagaaa cuucaugcag cugauccacg acgacagccu gaccuuuaaa     2220

gaggacaucc agaaagccca gguguccggc cagggcgaua gccugcacga gcacauugcc     2280

aaucuggccg gcagccccgc cauuaagaag ggcauccugc agacagugaa ggugguggac     2340

gagcucguga aagugauggg ccggcacaag cccgagaaca ucgugaucga aauggccaga     2400

gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaaugaa gcggaucgaa     2460

gagggcauca aagagcuggg cagccagauc cugaaagaac accccgugga aaacacccag     2520

cugcagaacg agaagcugua ccuguacuac cugcagaaug ggcgggauau guacguggac     2580

caggaacugg acaucaaccg gcuguccgac uacgaugugg accauaucgu gccucagagc     2640

uuucugaagg acgacuccau cgacaacaag gugcugacca gaagcgacaa gaaccggggc     2700

aagagcgaca acgugcccuc cgaagagguc gugaagaaga ugaagaacua cuggcggcag     2760

cugcugaacg ccaagcugau uacccagaga aaguucgaca aucugaccaa ggccgagaga     2820

ggcggccuga gcgaacugga uaaggccggc uucaucaaga gacagcuggu ggaaacccgg     2880

cagaucacaa agcacguggc acagauccug gacucccgga ugaacacuaa guacgacgag     2940

aaugacaagc ugauccggga agugaaagug aucacccuga aguccaagcu gguguccgau     3000

uuccggaagg auuuccaguu uuacaaagug cgcgagauca acaacuacca ccacgcccac     3060

gacgccuacc ugaacgccgu cgugggaacc gcccugauca aaaaguaccc uaagcuggaa     3120

agcgaguucg uguacggcga cuacaaggug uacgacgugc ggaagaugau cgccaagagc     3180

gagcaggaaa ucggcaaggc uaccgccaag uacuucuucu acagcaacau caugaacuuu     3240

uucaagaccg agauuacccu ggccaacggc gagauccgga agcggccucu gaucgagaca     3300

aacggcgaaa ccggggagau cgugugggau aagggccggg auuuugccac cgugcggaaa     3360

gugcugagca ugccccaagu gaauaucgug aaaaagaccg aggugcagac aggcggcuuc     3420

agcaaagagu cuauccugcc caagaggaac agcgauaagc ugaucgccag aaagaaggac     3480

ugggacccua agaaguacgg cggcuucgac agccccaccg uggccuauuc ugugcuggug     3540

guggccaaag uggaaaaggg caaguccaag aaacugaaga gugugaaaga gcugcugggg     3600

aucaccauca uggaaagaag cagcuucgag aagaauccca ucgacuuucu ggaagccaag     3660

ggcuacaaag aagugaaaaa ggaccugauc aucaagcugc cuaaguacuc ccuguucgag     3720

cuggaaaacg gccggaagag aaugcuggcc ucugccggcg aacugcagaa gggaaacgaa     3780

cuggcccugc ccuccaaaua ugugaacuuc cuguaccugg ccagccacua ugagaagcug     3840

aagggcuccc ccgaggauaa ugagcagaaa cagcuguuug uggaacagca caagcacuac     3900

cuggacgaga ucaucgagca gaucagcgag uucuccaaga gagugauccu ggccgacgcu     3960

aaucuggaca aagugcuguc cgccuacaac aagcaccggg auaagcccau cagagagcag     4020

gccgagaaua ucauccaccu guuuacccug accaaucugg gagccccugc cgccuucaag     4080

uacuuugaca ccaccaucga ccggaagagg uacaccagca ccaaagaggu gcuggacgcc     4140

acccugaucc accagagcau caccggccug uacgagacac ggaucgaccu gucucagcug     4200

ggaggcgaca agcguccugc ugcuacuaag aaagcugguc aagcuaagaa aaagaaauaa     4260

ggacuagugc aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau     4320

gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu     4380

cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa     4440

aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4530


<210>  419
<211>  4585
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  419
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

caaagaagaa gcggaagguc gguauccacg gagucccagc agccgacaag aaguacagca      120

ucggccugga caucggcacc aacucugugg gcugggccgu gaucaccgac gaguacaagg      180

ugcccagcaa gaaauucaag gugcugggca acaccgaccg gcacagcauc aagaagaacc      240

ugaucggagc ccugcuguuc gacagcggcg aaacagccga ggccacccgg cugaagagaa      300

ccgccagaag aagauacacc agacggaaga accggaucug cuaucugcaa gagaucuuca      360

gcaacgagau ggccaaggug gacgacagcu ucuuccacag acuggaagag uccuuccugg      420

uggaagagga uaagaagcac gagcggcacc ccaucuucgg caacaucgug gacgaggugg      480

ccuaccacga gaaguacccc accaucuacc accugagaaa gaaacuggug gacagcaccg      540

acaaggccga ccugcggcug aucuaucugg cccuggccca caugaucaag uuccggggcc      600

acuuccugau cgagggcgac cugaaccccg acaacagcga cguggacaag cuguucaucc      660

agcuggugca gaccuacaac cagcuguucg aggaaaaccc caucaacgcc agcggcgugg      720

acgccaaggc cauccugucu gccagacuga gcaagagcag acggcuggaa aaucugaucg      780

cccagcugcc cggcgagaag aagaauggcc uguucggcaa ccugauugcc cugagccugg      840

gccugacccc caacuucaag agcaacuucg accuggccga ggaugccaaa cugcagcuga      900

gcaaggacac cuacgacgac gaccuggaca accugcuggc ccagaucggc gaccaguacg      960

ccgaccuguu ucuggccgcc aagaaccugu ccgacgccau ccugcugagc gacauccuga     1020

gagugaacac cgagaucacc aaggcccccc ugagcgccuc uaugaucaag agauacgacg     1080

agcaccacca ggaccugacc cugcugaaag cucucgugcg gcagcagcug ccugagaagu     1140

acaaagagau uuucuucgac cagagcaaga acggcuacgc cggcuacauu gacggcggag     1200

ccagccagga agaguucuac aaguucauca agcccauccu ggaaaagaug gacggcaccg     1260

aggaacugcu cgugaagcug aacagagagg accugcugcg gaagcagcgg accuucgaca     1320

acggcagcau cccccaccag auccaccugg gagagcugca cgccauucug cggcggcagg     1380

aagauuuuua cccauuccug aaggacaacc gggaaaagau cgagaagauc cugaccuucc     1440

gcauccccua cuacgugggc ccucuggcca ggggaaacag cagauucgcc uggaugacca     1500

gaaagagcga ggaaaccauc acccccugga acuucgagga agugguggac aagggcgcuu     1560

ccgcccagag cuucaucgag cggaugacca acuucgauaa gaaccugccc aacgagaagg     1620

ugcugcccaa gcacagccug cuguacgagu acuucaccgu guauaacgag cugaccaaag     1680

ugaaauacgu gaccgaggga augagaaagc ccgccuuccu gagcggcgag cagaaaaagg     1740

ccaucgugga ccugcuguuc aagaccaacc ggaaagugac cgugaagcag cugaaagagg     1800

acuacuucaa gaaaaucgag ugcuucgacu ccguggaaau cuccggcgug gaagaucggu     1860

ucaacgccuc ccugggcaca uaccacgauc ugcugaaaau uaucaaggac aaggacuucc     1920

uggacaauga ggaaaacgag gacauucugg aagauaucgu gcugacccug acacuguuug     1980

aggacagaga gaugaucgag gaacggcuga aaaccuaugc ccaccuguuc gacgacaaag     2040

ugaugaagca gcugaagcgg cggagauaca ccggcugggg caggcugagc cggaagcuga     2100

ucaacggcau ccgggacaag caguccggca agacaauccu ggauuuccug aaguccgacg     2160

gcuucgccaa cagaaacuuc augcagcuga uccacgacga cagccugacc uuuaaagagg     2220

acauccagaa agcccaggug uccggccagg gcgauagccu gcacgagcac auugccaauc     2280

uggccggcag ccccgccauu aagaagggca uccugcagac agugaaggug guggacgagc     2340

ucgugaaagu gaugggccgg cacaagcccg agaacaucgu gaucgaaaug gccagagaga     2400

accagaccac ccagaaggga cagaagaaca gccgcgagag aaugaagcgg aucgaagagg     2460

gcaucaaaga gcugggcagc cagauccuga aagaacaccc cguggaaaac acccagcugc     2520

agaacgagaa gcuguaccug uacuaccugc agaaugggcg ggauauguac guggaccagg     2580

aacuggacau caaccggcug uccgacuacg auguggacca uaucgugccu cagagcuuuc     2640

ugaaggacga cuccaucgac aacaaggugc ugaccagaag cgacaagaac cggggcaaga     2700

gcgacaacgu gcccuccgaa gaggucguga agaagaugaa gaacuacugg cggcagcugc     2760

ugaacgccaa gcugauuacc cagagaaagu ucgacaaucu gaccaaggcc gagagaggcg     2820

gccugagcga acuggauaag gccggcuuca ucaagagaca gcugguggaa acccggcaga     2880

ucacaaagca cguggcacag auccuggacu cccggaugaa cacuaaguac gacgagaaug     2940

acaagcugau ccgggaagug aaagugauca cccugaaguc caagcuggug uccgauuucc     3000

ggaaggauuu ccaguuuuac aaagugcgcg agaucaacaa cuaccaccac gcccacgacg     3060

ccuaccugaa cgccgucgug ggaaccgccc ugaucaaaaa guacccuaag cuggaaagcg     3120

aguucgugua cggcgacuac aagguguacg acgugcggaa gaugaucgcc aagagcgagc     3180

aggaaaucgg caaggcuacc gccaaguacu ucuucuacag caacaucaug aacuuuuuca     3240

agaccgagau uacccuggcc aacggcgaga uccggaagcg gccucugauc gagacaaacg     3300

gcgaaaccgg ggagaucgug ugggauaagg gccgggauuu ugccaccgug cggaaagugc     3360

ugagcaugcc ccaagugaau aucgugaaaa agaccgaggu gcagacaggc ggcuucagca     3420

aagagucuau ccugcccaag aggaacagcg auaagcugau cgccagaaag aaggacuggg     3480

acccuaagaa guacggcggc uucgacagcc ccaccguggc cuauucugug cugguggugg     3540

ccaaagugga aaagggcaag uccaagaaac ugaagagugu gaaagagcug cuggggauca     3600

ccaucaugga aagaagcagc uucgagaaga aucccaucga cuuucuggaa gccaagggcu     3660

acaaagaagu gaaaaaggac cugaucauca agcugccuaa guacucccug uucgagcugg     3720

aaaacggccg gaagagaaug cuggccucug ccggcgaacu gcagaaggga aacgaacugg     3780

cccugcccuc caaauaugug aacuuccugu accuggccag ccacuaugag aagcugaagg     3840

gcucccccga ggauaaugag cagaaacagc uguuugugga acagcacaag cacuaccugg     3900

acgagaucau cgagcagauc agcgaguucu ccaagagagu gauccuggcc gacgcuaauc     3960

uggacaaagu gcuguccgcc uacaacaagc accgggauaa gcccaucaga gagcaggccg     4020

agaauaucau ccaccuguuu acccugacca aucugggagc cccugccgcc uucaaguacu     4080

uugacaccac caucgaccgg aagagguaca ccagcaccaa agaggugcug gacgccaccc     4140

ugauccacca gagcaucacc ggccuguacg agacacggau cgaccugucu cagcugggag     4200

gcgacaagcg uccugcugcu acuaagaaag cuggucaagc uaagaaaaag aaauaaggac     4260

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     4320

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     4380

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     4440

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     4560

ggcucuuuuc agagccacca gaauu                                           4585


<210>  420
<211>  4484
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  420
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gccccaaaga agaagcggaa ggucgguauc cacggagucc      120

cagcagccga caagaaguac agcaucggcc uggacaucgg caccaacucu gugggcuggg      180

ccgugaucac cgacgaguac aaggugccca gcaagaaauu caaggugcug ggcaacaccg      240

accggcacag caucaagaag aaccugaucg gagcccugcu guucgacagc ggcgaaacag      300

ccgaggccac ccggcugaag agaaccgcca gaagaagaua caccagacgg aagaaccgga      360

ucugcuaucu gcaagagauc uucagcaacg agauggccaa gguggacgac agcuucuucc      420

acagacugga agaguccuuc cugguggaag aggauaagaa gcacgagcgg caccccaucu      480

ucggcaacau cguggacgag guggccuacc acgagaagua ccccaccauc uaccaccuga      540

gaaagaaacu gguggacagc accgacaagg ccgaccugcg gcugaucuau cuggcccugg      600

cccacaugau caaguuccgg ggccacuucc ugaucgaggg cgaccugaac cccgacaaca      660

gcgacgugga caagcuguuc auccagcugg ugcagaccua caaccagcug uucgaggaaa      720

accccaucaa cgccagcggc guggacgcca aggccauccu gucugccaga cugagcaaga      780

gcagacggcu ggaaaaucug aucgcccagc ugcccggcga gaagaagaau ggccuguucg      840

gcaaccugau ugcccugagc cugggccuga cccccaacuu caagagcaac uucgaccugg      900

ccgaggaugc caaacugcag cugagcaagg acaccuacga cgacgaccug gacaaccugc      960

uggcccagau cggcgaccag uacgccgacc uguuucuggc cgccaagaac cuguccgacg     1020

ccauccugcu gagcgacauc cugagaguga acaccgagau caccaaggcc ccccugagcg     1080

ccucuaugau caagagauac gacgagcacc accaggaccu gacccugcug aaagcucucg     1140

ugcggcagca gcugccugag aaguacaaag agauuuucuu cgaccagagc aagaacggcu     1200

acgccggcua cauugacggc ggagccagcc aggaagaguu cuacaaguuc aucaagccca     1260

uccuggaaaa gauggacggc accgaggaac ugcucgugaa gcugaacaga gaggaccugc     1320

ugcggaagca gcggaccuuc gacaacggca gcauccccca ccagauccac cugggagagc     1380

ugcacgccau ucugcggcgg caggaagauu uuuacccauu ccugaaggac aaccgggaaa     1440

agaucgagaa gauccugacc uuccgcaucc ccuacuacgu gggcccucug gccaggggaa     1500

acagcagauu cgccuggaug accagaaaga gcgaggaaac caucaccccc uggaacuucg     1560

aggaaguggu ggacaagggc gcuuccgccc agagcuucau cgagcggaug accaacuucg     1620

auaagaaccu gcccaacgag aaggugcugc ccaagcacag ccugcuguac gaguacuuca     1680

ccguguauaa cgagcugacc aaagugaaau acgugaccga gggaaugaga aagcccgccu     1740

uccugagcgg cgagcagaaa aaggccaucg uggaccugcu guucaagacc aaccggaaag     1800

ugaccgugaa gcagcugaaa gaggacuacu ucaagaaaau cgagugcuuc gacuccgugg     1860

aaaucuccgg cguggaagau cgguucaacg ccucccuggg cacauaccac gaucugcuga     1920

aaauuaucaa ggacaaggac uuccuggaca augaggaaaa cgaggacauu cuggaagaua     1980

ucgugcugac ccugacacug uuugaggaca gagagaugau cgaggaacgg cugaaaaccu     2040

augcccaccu guucgacgac aaagugauga agcagcugaa gcggcggaga uacaccggcu     2100

ggggcaggcu gagccggaag cugaucaacg gcauccggga caagcagucc ggcaagacaa     2160

uccuggauuu ccugaagucc gacggcuucg ccaacagaaa cuucaugcag cugauccacg     2220

acgacagccu gaccuuuaaa gaggacaucc agaaagccca gguguccggc cagggcgaua     2280

gccugcacga gcacauugcc aaucuggccg gcagccccgc cauuaagaag ggcauccugc     2340

agacagugaa ggugguggac gagcucguga aagugauggg ccggcacaag cccgagaaca     2400

ucgugaucga aauggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg     2460

agagaaugaa gcggaucgaa gagggcauca aagagcuggg cagccagauc cugaaagaac     2520

accccgugga aaacacccag cugcagaacg agaagcugua ccuguacuac cugcagaaug     2580

ggcgggauau guacguggac caggaacugg acaucaaccg gcuguccgac uacgaugugg     2640

accauaucgu gccucagagc uuucugaagg acgacuccau cgacaacaag gugcugacca     2700

gaagcgacaa gaaccggggc aagagcgaca acgugcccuc cgaagagguc gugaagaaga     2760

ugaagaacua cuggcggcag cugcugaacg ccaagcugau uacccagaga aaguucgaca     2820

aucugaccaa ggccgagaga ggcggccuga gcgaacugga uaaggccggc uucaucaaga     2880

gacagcuggu ggaaacccgg cagaucacaa agcacguggc acagauccug gacucccgga     2940

ugaacacuaa guacgacgag aaugacaagc ugauccggga agugaaagug aucacccuga     3000

aguccaagcu gguguccgau uuccggaagg auuuccaguu uuacaaagug cgcgagauca     3060

acaacuacca ccacgcccac gacgccuacc ugaacgccgu cgugggaacc gcccugauca     3120

aaaaguaccc uaagcuggaa agcgaguucg uguacggcga cuacaaggug uacgacgugc     3180

ggaagaugau cgccaagagc gagcaggaaa ucggcaaggc uaccgccaag uacuucuucu     3240

acagcaacau caugaacuuu uucaagaccg agauuacccu ggccaacggc gagauccgga     3300

agcggccucu gaucgagaca aacggcgaaa ccggggagau cgugugggau aagggccggg     3360

auuuugccac cgugcggaaa gugcugagca ugccccaagu gaauaucgug aaaaagaccg     3420

aggugcagac aggcggcuuc agcaaagagu cuauccugcc caagaggaac agcgauaagc     3480

ugaucgccag aaagaaggac ugggacccua agaaguacgg cggcuucgac agccccaccg     3540

uggccuauuc ugugcuggug guggccaaag uggaaaaggg caaguccaag aaacugaaga     3600

gugugaaaga gcugcugggg aucaccauca uggaaagaag cagcuucgag aagaauccca     3660

ucgacuuucu ggaagccaag ggcuacaaag aagugaaaaa ggaccugauc aucaagcugc     3720

cuaaguacuc ccuguucgag cuggaaaacg gccggaagag aaugcuggcc ucugccggcg     3780

aacugcagaa gggaaacgaa cuggcccugc ccuccaaaua ugugaacuuc cuguaccugg     3840

ccagccacua ugagaagcug aagggcuccc ccgaggauaa ugagcagaaa cagcuguuug     3900

uggaacagca caagcacuac cuggacgaga ucaucgagca gaucagcgag uucuccaaga     3960

gagugauccu ggccgacgcu aaucuggaca aagugcuguc cgccuacaac aagcaccggg     4020

auaagcccau cagagagcag gccgagaaua ucauccaccu guuuacccug accaaucugg     4080

gagccccugc cgccuucaag uacuuugaca ccaccaucga ccggaagagg uacaccagca     4140

ccaaagaggu gcuggacgcc acccugaucc accagagcau caccggccug uacgagacac     4200

ggaucgaccu gucucagcug ggaggcgaca agcguccugc ugcuacuaag aaagcugguc     4260

aagcuaagaa aaagaaauaa ggacuaguaa uaaggaaacu guaugaaugu cugugggcag     4320

gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu     4380

uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4484


<210>  421
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  421
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

ccaaagaaga agcggaaggu cgguauccac ggagucccag cagccgacaa gaaguacagc      240

aucggccugg acaucggcac caacucugug ggcugggccg ugaucaccga cgaguacaag      300

gugcccagca agaaauucaa ggugcugggc aacaccgacc ggcacagcau caagaagaac      360

cugaucggag cccugcuguu cgacagcggc gaaacagccg aggccacccg gcugaagaga      420

accgccagaa gaagauacac cagacggaag aaccggaucu gcuaucugca agagaucuuc      480

agcaacgaga uggccaaggu ggacgacagc uucuuccaca gacuggaaga guccuuccug      540

guggaagagg auaagaagca cgagcggcac cccaucuucg gcaacaucgu ggacgaggug      600

gccuaccacg agaaguaccc caccaucuac caccugagaa agaaacuggu ggacagcacc      660

gacaaggccg accugcggcu gaucuaucug gcccuggccc acaugaucaa guuccggggc      720

cacuuccuga ucgagggcga ccugaacccc gacaacagcg acguggacaa gcuguucauc      780

cagcuggugc agaccuacaa ccagcuguuc gaggaaaacc ccaucaacgc cagcggcgug      840

gacgccaagg ccauccuguc ugccagacug agcaagagca gacggcugga aaaucugauc      900

gcccagcugc ccggcgagaa gaagaauggc cuguucggca accugauugc ccugagccug      960

ggccugaccc ccaacuucaa gagcaacuuc gaccuggccg aggaugccaa acugcagcug     1020

agcaaggaca ccuacgacga cgaccuggac aaccugcugg cccagaucgg cgaccaguac     1080

gccgaccugu uucuggccgc caagaaccug uccgacgcca uccugcugag cgacauccug     1140

agagugaaca ccgagaucac caaggccccc cugagcgccu cuaugaucaa gagauacgac     1200

gagcaccacc aggaccugac ccugcugaaa gcucucgugc ggcagcagcu gccugagaag     1260

uacaaagaga uuuucuucga ccagagcaag aacggcuacg ccggcuacau ugacggcgga     1320

gccagccagg aagaguucua caaguucauc aagcccaucc uggaaaagau ggacggcacc     1380

gaggaacugc ucgugaagcu gaacagagag gaccugcugc ggaagcagcg gaccuucgac     1440

aacggcagca ucccccacca gauccaccug ggagagcugc acgccauucu gcggcggcag     1500

gaagauuuuu acccauuccu gaaggacaac cgggaaaaga ucgagaagau ccugaccuuc     1560

cgcauccccu acuacguggg cccucuggcc aggggaaaca gcagauucgc cuggaugacc     1620

agaaagagcg aggaaaccau cacccccugg aacuucgagg aaguggugga caagggcgcu     1680

uccgcccaga gcuucaucga gcggaugacc aacuucgaua agaaccugcc caacgagaag     1740

gugcugccca agcacagccu gcuguacgag uacuucaccg uguauaacga gcugaccaaa     1800

gugaaauacg ugaccgaggg aaugagaaag cccgccuucc ugagcggcga gcagaaaaag     1860

gccaucgugg accugcuguu caagaccaac cggaaaguga ccgugaagca gcugaaagag     1920

gacuacuuca agaaaaucga gugcuucgac uccguggaaa ucuccggcgu ggaagaucgg     1980

uucaacgccu cccugggcac auaccacgau cugcugaaaa uuaucaagga caaggacuuc     2040

cuggacaaug aggaaaacga ggacauucug gaagauaucg ugcugacccu gacacuguuu     2100

gaggacagag agaugaucga ggaacggcug aaaaccuaug cccaccuguu cgacgacaaa     2160

gugaugaagc agcugaagcg gcggagauac accggcuggg gcaggcugag ccggaagcug     2220

aucaacggca uccgggacaa gcaguccggc aagacaaucc uggauuuccu gaaguccgac     2280

ggcuucgcca acagaaacuu caugcagcug auccacgacg acagccugac cuuuaaagag     2340

gacauccaga aagcccaggu guccggccag ggcgauagcc ugcacgagca cauugccaau     2400

cuggccggca gccccgccau uaagaagggc auccugcaga cagugaaggu gguggacgag     2460

cucgugaaag ugaugggccg gcacaagccc gagaacaucg ugaucgaaau ggccagagag     2520

aaccagacca cccagaaggg acagaagaac agccgcgaga gaaugaagcg gaucgaagag     2580

ggcaucaaag agcugggcag ccagauccug aaagaacacc ccguggaaaa cacccagcug     2640

cagaacgaga agcuguaccu guacuaccug cagaaugggc gggauaugua cguggaccag     2700

gaacuggaca ucaaccggcu guccgacuac gauguggacc auaucgugcc ucagagcuuu     2760

cugaaggacg acuccaucga caacaaggug cugaccagaa gcgacaagaa ccggggcaag     2820

agcgacaacg ugcccuccga agaggucgug aagaagauga agaacuacug gcggcagcug     2880

cugaacgcca agcugauuac ccagagaaag uucgacaauc ugaccaaggc cgagagaggc     2940

ggccugagcg aacuggauaa ggccggcuuc aucaagagac agcuggugga aacccggcag     3000

aucacaaagc acguggcaca gauccuggac ucccggauga acacuaagua cgacgagaau     3060

gacaagcuga uccgggaagu gaaagugauc acccugaagu ccaagcuggu guccgauuuc     3120

cggaaggauu uccaguuuua caaagugcgc gagaucaaca acuaccacca cgcccacgac     3180

gccuaccuga acgccgucgu gggaaccgcc cugaucaaaa aguacccuaa gcuggaaagc     3240

gaguucgugu acggcgacua caagguguac gacgugcgga agaugaucgc caagagcgag     3300

caggaaaucg gcaaggcuac cgccaaguac uucuucuaca gcaacaucau gaacuuuuuc     3360

aagaccgaga uuacccuggc caacggcgag auccggaagc ggccucugau cgagacaaac     3420

ggcgaaaccg gggagaucgu gugggauaag ggccgggauu uugccaccgu gcggaaagug     3480

cugagcaugc cccaagugaa uaucgugaaa aagaccgagg ugcagacagg cggcuucagc     3540

aaagagucua uccugcccaa gaggaacagc gauaagcuga ucgccagaaa gaaggacugg     3600

gacccuaaga aguacggcgg cuucgacagc cccaccgugg ccuauucugu gcugguggug     3660

gccaaagugg aaaagggcaa guccaagaaa cugaagagug ugaaagagcu gcuggggauc     3720

accaucaugg aaagaagcag cuucgagaag aaucccaucg acuuucugga agccaagggc     3780

uacaaagaag ugaaaaagga ccugaucauc aagcugccua aguacucccu guucgagcug     3840

gaaaacggcc ggaagagaau gcuggccucu gccggcgaac ugcagaaggg aaacgaacug     3900

gcccugcccu ccaaauaugu gaacuuccug uaccuggcca gccacuauga gaagcugaag     3960

ggcucccccg aggauaauga gcagaaacag cuguuugugg aacagcacaa gcacuaccug     4020

gacgagauca ucgagcagau cagcgaguuc uccaagagag ugauccuggc cgacgcuaau     4080

cuggacaaag ugcuguccgc cuacaacaag caccgggaua agcccaucag agagcaggcc     4140

gagaauauca uccaccuguu uacccugacc aaucugggag ccccugccgc cuucaaguac     4200

uuugacacca ccaucgaccg gaagagguac accagcacca aagaggugcu ggacgccacc     4260

cugauccacc agagcaucac cggccuguac gagacacgga ucgaccuguc ucagcuggga     4320

ggcgacaagc guccugcugc uacuaagaaa gcuggucaag cuaagaaaaa gaaauaagga     4380

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     4440

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     4500

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaagaau u                                               4581


<210>  422
<211>  4517
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  422
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

ccaaagaaga agcggaaggu cgguauccac ggagucccag cagccgacaa gaaguacagc      240

aucggccugg acaucggcac caacucugug ggcugggccg ugaucaccga cgaguacaag      300

gugcccagca agaaauucaa ggugcugggc aacaccgacc ggcacagcau caagaagaac      360

cugaucggag cccugcuguu cgacagcggc gaaacagccg aggccacccg gcugaagaga      420

accgccagaa gaagauacac cagacggaag aaccggaucu gcuaucugca agagaucuuc      480

agcaacgaga uggccaaggu ggacgacagc uucuuccaca gacuggaaga guccuuccug      540

guggaagagg auaagaagca cgagcggcac cccaucuucg gcaacaucgu ggacgaggug      600

gccuaccacg agaaguaccc caccaucuac caccugagaa agaaacuggu ggacagcacc      660

gacaaggccg accugcggcu gaucuaucug gcccuggccc acaugaucaa guuccggggc      720

cacuuccuga ucgagggcga ccugaacccc gacaacagcg acguggacaa gcuguucauc      780

cagcuggugc agaccuacaa ccagcuguuc gaggaaaacc ccaucaacgc cagcggcgug      840

gacgccaagg ccauccuguc ugccagacug agcaagagca gacggcugga aaaucugauc      900

gcccagcugc ccggcgagaa gaagaauggc cuguucggca accugauugc ccugagccug      960

ggccugaccc ccaacuucaa gagcaacuuc gaccuggccg aggaugccaa acugcagcug     1020

agcaaggaca ccuacgacga cgaccuggac aaccugcugg cccagaucgg cgaccaguac     1080

gccgaccugu uucuggccgc caagaaccug uccgacgcca uccugcugag cgacauccug     1140

agagugaaca ccgagaucac caaggccccc cugagcgccu cuaugaucaa gagauacgac     1200

gagcaccacc aggaccugac ccugcugaaa gcucucgugc ggcagcagcu gccugagaag     1260

uacaaagaga uuuucuucga ccagagcaag aacggcuacg ccggcuacau ugacggcgga     1320

gccagccagg aagaguucua caaguucauc aagcccaucc uggaaaagau ggacggcacc     1380

gaggaacugc ucgugaagcu gaacagagag gaccugcugc ggaagcagcg gaccuucgac     1440

aacggcagca ucccccacca gauccaccug ggagagcugc acgccauucu gcggcggcag     1500

gaagauuuuu acccauuccu gaaggacaac cgggaaaaga ucgagaagau ccugaccuuc     1560

cgcauccccu acuacguggg cccucuggcc aggggaaaca gcagauucgc cuggaugacc     1620

agaaagagcg aggaaaccau cacccccugg aacuucgagg aaguggugga caagggcgcu     1680

uccgcccaga gcuucaucga gcggaugacc aacuucgaua agaaccugcc caacgagaag     1740

gugcugccca agcacagccu gcuguacgag uacuucaccg uguauaacga gcugaccaaa     1800

gugaaauacg ugaccgaggg aaugagaaag cccgccuucc ugagcggcga gcagaaaaag     1860

gccaucgugg accugcuguu caagaccaac cggaaaguga ccgugaagca gcugaaagag     1920

gacuacuuca agaaaaucga gugcuucgac uccguggaaa ucuccggcgu ggaagaucgg     1980

uucaacgccu cccugggcac auaccacgau cugcugaaaa uuaucaagga caaggacuuc     2040

cuggacaaug aggaaaacga ggacauucug gaagauaucg ugcugacccu gacacuguuu     2100

gaggacagag agaugaucga ggaacggcug aaaaccuaug cccaccuguu cgacgacaaa     2160

gugaugaagc agcugaagcg gcggagauac accggcuggg gcaggcugag ccggaagcug     2220

aucaacggca uccgggacaa gcaguccggc aagacaaucc uggauuuccu gaaguccgac     2280

ggcuucgcca acagaaacuu caugcagcug auccacgacg acagccugac cuuuaaagag     2340

gacauccaga aagcccaggu guccggccag ggcgauagcc ugcacgagca cauugccaau     2400

cuggccggca gccccgccau uaagaagggc auccugcaga cagugaaggu gguggacgag     2460

cucgugaaag ugaugggccg gcacaagccc gagaacaucg ugaucgaaau ggccagagag     2520

aaccagacca cccagaaggg acagaagaac agccgcgaga gaaugaagcg gaucgaagag     2580

ggcaucaaag agcugggcag ccagauccug aaagaacacc ccguggaaaa cacccagcug     2640

cagaacgaga agcuguaccu guacuaccug cagaaugggc gggauaugua cguggaccag     2700

gaacuggaca ucaaccggcu guccgacuac gauguggacc auaucgugcc ucagagcuuu     2760

cugaaggacg acuccaucga caacaaggug cugaccagaa gcgacaagaa ccggggcaag     2820

agcgacaacg ugcccuccga agaggucgug aagaagauga agaacuacug gcggcagcug     2880

cugaacgcca agcugauuac ccagagaaag uucgacaauc ugaccaaggc cgagagaggc     2940

ggccugagcg aacuggauaa ggccggcuuc aucaagagac agcuggugga aacccggcag     3000

aucacaaagc acguggcaca gauccuggac ucccggauga acacuaagua cgacgagaau     3060

gacaagcuga uccgggaagu gaaagugauc acccugaagu ccaagcuggu guccgauuuc     3120

cggaaggauu uccaguuuua caaagugcgc gagaucaaca acuaccacca cgcccacgac     3180

gccuaccuga acgccgucgu gggaaccgcc cugaucaaaa aguacccuaa gcuggaaagc     3240

gaguucgugu acggcgacua caagguguac gacgugcgga agaugaucgc caagagcgag     3300

caggaaaucg gcaaggcuac cgccaaguac uucuucuaca gcaacaucau gaacuuuuuc     3360

aagaccgaga uuacccuggc caacggcgag auccggaagc ggccucugau cgagacaaac     3420

ggcgaaaccg gggagaucgu gugggauaag ggccgggauu uugccaccgu gcggaaagug     3480

cugagcaugc cccaagugaa uaucgugaaa aagaccgagg ugcagacagg cggcuucagc     3540

aaagagucua uccugcccaa gaggaacagc gauaagcuga ucgccagaaa gaaggacugg     3600

gacccuaaga aguacggcgg cuucgacagc cccaccgugg ccuauucugu gcugguggug     3660

gccaaagugg aaaagggcaa guccaagaaa cugaagagug ugaaagagcu gcuggggauc     3720

accaucaugg aaagaagcag cuucgagaag aaucccaucg acuuucugga agccaagggc     3780

uacaaagaag ugaaaaagga ccugaucauc aagcugccua aguacucccu guucgagcug     3840

gaaaacggcc ggaagagaau gcuggccucu gccggcgaac ugcagaaggg aaacgaacug     3900

gcccugcccu ccaaauaugu gaacuuccug uaccuggcca gccacuauga gaagcugaag     3960

ggcucccccg aggauaauga gcagaaacag cuguuugugg aacagcacaa gcacuaccug     4020

gacgagauca ucgagcagau cagcgaguuc uccaagagag ugauccuggc cgacgcuaau     4080

cuggacaaag ugcuguccgc cuacaacaag caccgggaua agcccaucag agagcaggcc     4140

gagaauauca uccaccuguu uacccugacc aaucugggag ccccugccgc cuucaaguac     4200

uuugacacca ccaucgaccg gaagagguac accagcacca aagaggugcu ggacgccacc     4260

cugauccacc agagcaucac cggccuguac gagacacgga ucgaccuguc ucagcuggga     4320

ggcgacaagc guccugcugc uacuaagaaa gcuggucaag cuaagaaaaa gaaauaagga     4380

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4440

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aagaauu                                                    4517


<210>  423
<211>  4466
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  423
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc caaagaagaa gcggaagguc gguauccacg gagucccagc agccgacaag      180

aaguacagca ucggccugga caucggcacc aacucugugg gcugggccgu gaucaccgac      240

gaguacaagg ugcccagcaa gaaauucaag gugcugggca acaccgaccg gcacagcauc      300

aagaagaacc ugaucggagc ccugcuguuc gacagcggcg aaacagccga ggccacccgg      360

cugaagagaa ccgccagaag aagauacacc agacggaaga accggaucug cuaucugcaa      420

gagaucuuca gcaacgagau ggccaaggug gacgacagcu ucuuccacag acuggaagag      480

uccuuccugg uggaagagga uaagaagcac gagcggcacc ccaucuucgg caacaucgug      540

gacgaggugg ccuaccacga gaaguacccc accaucuacc accugagaaa gaaacuggug      600

gacagcaccg acaaggccga ccugcggcug aucuaucugg cccuggccca caugaucaag      660

uuccggggcc acuuccugau cgagggcgac cugaaccccg acaacagcga cguggacaag      720

cuguucaucc agcuggugca gaccuacaac cagcuguucg aggaaaaccc caucaacgcc      780

agcggcgugg acgccaaggc cauccugucu gccagacuga gcaagagcag acggcuggaa      840

aaucugaucg cccagcugcc cggcgagaag aagaauggcc uguucggcaa ccugauugcc      900

cugagccugg gccugacccc caacuucaag agcaacuucg accuggccga ggaugccaaa      960

cugcagcuga gcaaggacac cuacgacgac gaccuggaca accugcuggc ccagaucggc     1020

gaccaguacg ccgaccuguu ucuggccgcc aagaaccugu ccgacgccau ccugcugagc     1080

gacauccuga gagugaacac cgagaucacc aaggcccccc ugagcgccuc uaugaucaag     1140

agauacgacg agcaccacca ggaccugacc cugcugaaag cucucgugcg gcagcagcug     1200

ccugagaagu acaaagagau uuucuucgac cagagcaaga acggcuacgc cggcuacauu     1260

gacggcggag ccagccagga agaguucuac aaguucauca agcccauccu ggaaaagaug     1320

gacggcaccg aggaacugcu cgugaagcug aacagagagg accugcugcg gaagcagcgg     1380

accuucgaca acggcagcau cccccaccag auccaccugg gagagcugca cgccauucug     1440

cggcggcagg aagauuuuua cccauuccug aaggacaacc gggaaaagau cgagaagauc     1500

cugaccuucc gcauccccua cuacgugggc ccucuggcca ggggaaacag cagauucgcc     1560

uggaugacca gaaagagcga ggaaaccauc acccccugga acuucgagga agugguggac     1620

aagggcgcuu ccgcccagag cuucaucgag cggaugacca acuucgauaa gaaccugccc     1680

aacgagaagg ugcugcccaa gcacagccug cuguacgagu acuucaccgu guauaacgag     1740

cugaccaaag ugaaauacgu gaccgaggga augagaaagc ccgccuuccu gagcggcgag     1800

cagaaaaagg ccaucgugga ccugcuguuc aagaccaacc ggaaagugac cgugaagcag     1860

cugaaagagg acuacuucaa gaaaaucgag ugcuucgacu ccguggaaau cuccggcgug     1920

gaagaucggu ucaacgccuc ccugggcaca uaccacgauc ugcugaaaau uaucaaggac     1980

aaggacuucc uggacaauga ggaaaacgag gacauucugg aagauaucgu gcugacccug     2040

acacuguuug aggacagaga gaugaucgag gaacggcuga aaaccuaugc ccaccuguuc     2100

gacgacaaag ugaugaagca gcugaagcgg cggagauaca ccggcugggg caggcugagc     2160

cggaagcuga ucaacggcau ccgggacaag caguccggca agacaauccu ggauuuccug     2220

aaguccgacg gcuucgccaa cagaaacuuc augcagcuga uccacgacga cagccugacc     2280

uuuaaagagg acauccagaa agcccaggug uccggccagg gcgauagccu gcacgagcac     2340

auugccaauc uggccggcag ccccgccauu aagaagggca uccugcagac agugaaggug     2400

guggacgagc ucgugaaagu gaugggccgg cacaagcccg agaacaucgu gaucgaaaug     2460

gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aaugaagcgg     2520

aucgaagagg gcaucaaaga gcugggcagc cagauccuga aagaacaccc cguggaaaac     2580

acccagcugc agaacgagaa gcuguaccug uacuaccugc agaaugggcg ggauauguac     2640

guggaccagg aacuggacau caaccggcug uccgacuacg auguggacca uaucgugccu     2700

cagagcuuuc ugaaggacga cuccaucgac aacaaggugc ugaccagaag cgacaagaac     2760

cggggcaaga gcgacaacgu gcccuccgaa gaggucguga agaagaugaa gaacuacugg     2820

cggcagcugc ugaacgccaa gcugauuacc cagagaaagu ucgacaaucu gaccaaggcc     2880

gagagaggcg gccugagcga acuggauaag gccggcuuca ucaagagaca gcugguggaa     2940

acccggcaga ucacaaagca cguggcacag auccuggacu cccggaugaa cacuaaguac     3000

gacgagaaug acaagcugau ccgggaagug aaagugauca cccugaaguc caagcuggug     3060

uccgauuucc ggaaggauuu ccaguuuuac aaagugcgcg agaucaacaa cuaccaccac     3120

gcccacgacg ccuaccugaa cgccgucgug ggaaccgccc ugaucaaaaa guacccuaag     3180

cuggaaagcg aguucgugua cggcgacuac aagguguacg acgugcggaa gaugaucgcc     3240

aagagcgagc aggaaaucgg caaggcuacc gccaaguacu ucuucuacag caacaucaug     3300

aacuuuuuca agaccgagau uacccuggcc aacggcgaga uccggaagcg gccucugauc     3360

gagacaaacg gcgaaaccgg ggagaucgug ugggauaagg gccgggauuu ugccaccgug     3420

cggaaagugc ugagcaugcc ccaagugaau aucgugaaaa agaccgaggu gcagacaggc     3480

ggcuucagca aagagucuau ccugcccaag aggaacagcg auaagcugau cgccagaaag     3540

aaggacuggg acccuaagaa guacggcggc uucgacagcc ccaccguggc cuauucugug     3600

cugguggugg ccaaagugga aaagggcaag uccaagaaac ugaagagugu gaaagagcug     3660

cuggggauca ccaucaugga aagaagcagc uucgagaaga aucccaucga cuuucuggaa     3720

gccaagggcu acaaagaagu gaaaaaggac cugaucauca agcugccuaa guacucccug     3780

uucgagcugg aaaacggccg gaagagaaug cuggccucug ccggcgaacu gcagaaggga     3840

aacgaacugg cccugcccuc caaauaugug aacuuccugu accuggccag ccacuaugag     3900

aagcugaagg gcucccccga ggauaaugag cagaaacagc uguuugugga acagcacaag     3960

cacuaccugg acgagaucau cgagcagauc agcgaguucu ccaagagagu gauccuggcc     4020

gacgcuaauc uggacaaagu gcuguccgcc uacaacaagc accgggauaa gcccaucaga     4080

gagcaggccg agaauaucau ccaccuguuu acccugacca aucugggagc cccugccgcc     4140

uucaaguacu uugacaccac caucgaccgg aagagguaca ccagcaccaa agaggugcug     4200

gacgccaccc ugauccacca gagcaucacc ggccuguacg agacacggau cgaccugucu     4260

cagcugggag gcgacaagcg uccugcugcu acuaagaaag cuggucaagc uaagaaaaag     4320

aaauaaggac uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua     4380

gccugucuuu cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa agaauu                                          4466


<210>  424
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  424
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg ccccaaagaa gaagcggaag      120

gucgguaucc acggaguccc agcagccgac aagaaguaca gcaucggccu ggacaucggc      180

accaacucug ugggcugggc cgugaucacc gacgaguaca aggugcccag caagaaauuc      240

aaggugcugg gcaacaccga ccggcacagc aucaagaaga accugaucgg agcccugcug      300

uucgacagcg gcgaaacagc cgaggccacc cggcugaaga gaaccgccag aagaagauac      360

accagacgga agaaccggau cugcuaucug caagagaucu ucagcaacga gauggccaag      420

guggacgaca gcuucuucca cagacuggaa gaguccuucc ugguggaaga ggauaagaag      480

cacgagcggc accccaucuu cggcaacauc guggacgagg uggccuacca cgagaaguac      540

cccaccaucu accaccugag aaagaaacug guggacagca ccgacaaggc cgaccugcgg      600

cugaucuauc uggcccuggc ccacaugauc aaguuccggg gccacuuccu gaucgagggc      660

gaccugaacc ccgacaacag cgacguggac aagcuguuca uccagcuggu gcagaccuac      720

aaccagcugu ucgaggaaaa ccccaucaac gccagcggcg uggacgccaa ggccauccug      780

ucugccagac ugagcaagag cagacggcug gaaaaucuga ucgcccagcu gcccggcgag      840

aagaagaaug gccuguucgg caaccugauu gcccugagcc ugggccugac ccccaacuuc      900

aagagcaacu ucgaccuggc cgaggaugcc aaacugcagc ugagcaagga caccuacgac      960

gacgaccugg acaaccugcu ggcccagauc ggcgaccagu acgccgaccu guuucuggcc     1020

gccaagaacc uguccgacgc cauccugcug agcgacaucc ugagagugaa caccgagauc     1080

accaaggccc cccugagcgc cucuaugauc aagagauacg acgagcacca ccaggaccug     1140

acccugcuga aagcucucgu gcggcagcag cugccugaga aguacaaaga gauuuucuuc     1200

gaccagagca agaacggcua cgccggcuac auugacggcg gagccagcca ggaagaguuc     1260

uacaaguuca ucaagcccau ccuggaaaag auggacggca ccgaggaacu gcucgugaag     1320

cugaacagag aggaccugcu gcggaagcag cggaccuucg acaacggcag caucccccac     1380

cagauccacc ugggagagcu gcacgccauu cugcggcggc aggaagauuu uuacccauuc     1440

cugaaggaca accgggaaaa gaucgagaag auccugaccu uccgcauccc cuacuacgug     1500

ggcccucugg ccaggggaaa cagcagauuc gccuggauga ccagaaagag cgaggaaacc     1560

aucacccccu ggaacuucga ggaaguggug gacaagggcg cuuccgccca gagcuucauc     1620

gagcggauga ccaacuucga uaagaaccug cccaacgaga aggugcugcc caagcacagc     1680

cugcuguacg aguacuucac cguguauaac gagcugacca aagugaaaua cgugaccgag     1740

ggaaugagaa agcccgccuu ccugagcggc gagcagaaaa aggccaucgu ggaccugcug     1800

uucaagacca accggaaagu gaccgugaag cagcugaaag aggacuacuu caagaaaauc     1860

gagugcuucg acuccgugga aaucuccggc guggaagauc gguucaacgc cucccugggc     1920

acauaccacg aucugcugaa aauuaucaag gacaaggacu uccuggacaa ugaggaaaac     1980

gaggacauuc uggaagauau cgugcugacc cugacacugu uugaggacag agagaugauc     2040

gaggaacggc ugaaaaccua ugcccaccug uucgacgaca aagugaugaa gcagcugaag     2100

cggcggagau acaccggcug gggcaggcug agccggaagc ugaucaacgg cauccgggac     2160

aagcaguccg gcaagacaau ccuggauuuc cugaaguccg acggcuucgc caacagaaac     2220

uucaugcagc ugauccacga cgacagccug accuuuaaag aggacaucca gaaagcccag     2280

guguccggcc agggcgauag ccugcacgag cacauugcca aucuggccgg cagccccgcc     2340

auuaagaagg gcauccugca gacagugaag gugguggacg agcucgugaa agugaugggc     2400

cggcacaagc ccgagaacau cgugaucgaa auggccagag agaaccagac cacccagaag     2460

ggacagaaga acagccgcga gagaaugaag cggaucgaag agggcaucaa agagcugggc     2520

agccagaucc ugaaagaaca ccccguggaa aacacccagc ugcagaacga gaagcuguac     2580

cuguacuacc ugcagaaugg gcgggauaug uacguggacc aggaacugga caucaaccgg     2640

cuguccgacu acgaugugga ccauaucgug ccucagagcu uucugaagga cgacuccauc     2700

gacaacaagg ugcugaccag aagcgacaag aaccggggca agagcgacaa cgugcccucc     2760

gaagaggucg ugaagaagau gaagaacuac uggcggcagc ugcugaacgc caagcugauu     2820

acccagagaa aguucgacaa ucugaccaag gccgagagag gcggccugag cgaacuggau     2880

aaggccggcu ucaucaagag acagcuggug gaaacccggc agaucacaaa gcacguggca     2940

cagauccugg acucccggau gaacacuaag uacgacgaga augacaagcu gauccgggaa     3000

gugaaaguga ucacccugaa guccaagcug guguccgauu uccggaagga uuuccaguuu     3060

uacaaagugc gcgagaucaa caacuaccac cacgcccacg acgccuaccu gaacgccguc     3120

gugggaaccg cccugaucaa aaaguacccu aagcuggaaa gcgaguucgu guacggcgac     3180

uacaaggugu acgacgugcg gaagaugauc gccaagagcg agcaggaaau cggcaaggcu     3240

accgccaagu acuucuucua cagcaacauc augaacuuuu ucaagaccga gauuacccug     3300

gccaacggcg agauccggaa gcggccucug aucgagacaa acggcgaaac cggggagauc     3360

gugugggaua agggccggga uuuugccacc gugcggaaag ugcugagcau gccccaagug     3420

aauaucguga aaaagaccga ggugcagaca ggcggcuuca gcaaagaguc uauccugccc     3480

aagaggaaca gcgauaagcu gaucgccaga aagaaggacu gggacccuaa gaaguacggc     3540

ggcuucgaca gccccaccgu ggccuauucu gugcuggugg uggccaaagu ggaaaagggc     3600

aaguccaaga aacugaagag ugugaaagag cugcugggga ucaccaucau ggaaagaagc     3660

agcuucgaga agaaucccau cgacuuucug gaagccaagg gcuacaaaga agugaaaaag     3720

gaccugauca ucaagcugcc uaaguacucc cuguucgagc uggaaaacgg ccggaagaga     3780

augcuggccu cugccggcga acugcagaag ggaaacgaac uggcccugcc cuccaaauau     3840

gugaacuucc uguaccuggc cagccacuau gagaagcuga agggcucccc cgaggauaau     3900

gagcagaaac agcuguuugu ggaacagcac aagcacuacc uggacgagau caucgagcag     3960

aucagcgagu ucuccaagag agugauccug gccgacgcua aucuggacaa agugcugucc     4020

gccuacaaca agcaccggga uaagcccauc agagagcagg ccgagaauau cauccaccug     4080

uuuacccuga ccaaucuggg agccccugcc gccuucaagu acuuugacac caccaucgac     4140

cggaagaggu acaccagcac caaagaggug cuggacgcca cccugaucca ccagagcauc     4200

accggccugu acgagacacg gaucgaccug ucucagcugg gaggcgacaa gcguccugcu     4260

gcuacuaaga aagcugguca agcuaagaaa aagaaauaag gacuaguguc caccuguccc     4320

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     4380

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaagaa uu                                                         4452


<210>  425
<211>  4433
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  425
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gccccaaaga agaagcggaa ggucgguauc cacggagucc      120

cagcagccga caagaaguac agcaucggcc uggacaucgg caccaacucu gugggcuggg      180

ccgugaucac cgacgaguac aaggugccca gcaagaaauu caaggugcug ggcaacaccg      240

accggcacag caucaagaag aaccugaucg gagcccugcu guucgacagc ggcgaaacag      300

ccgaggccac ccggcugaag agaaccgcca gaagaagaua caccagacgg aagaaccgga      360

ucugcuaucu gcaagagauc uucagcaacg agauggccaa gguggacgac agcuucuucc      420

acagacugga agaguccuuc cugguggaag aggauaagaa gcacgagcgg caccccaucu      480

ucggcaacau cguggacgag guggccuacc acgagaagua ccccaccauc uaccaccuga      540

gaaagaaacu gguggacagc accgacaagg ccgaccugcg gcugaucuau cuggcccugg      600

cccacaugau caaguuccgg ggccacuucc ugaucgaggg cgaccugaac cccgacaaca      660

gcgacgugga caagcuguuc auccagcugg ugcagaccua caaccagcug uucgaggaaa      720

accccaucaa cgccagcggc guggacgcca aggccauccu gucugccaga cugagcaaga      780

gcagacggcu ggaaaaucug aucgcccagc ugcccggcga gaagaagaau ggccuguucg      840

gcaaccugau ugcccugagc cugggccuga cccccaacuu caagagcaac uucgaccugg      900

ccgaggaugc caaacugcag cugagcaagg acaccuacga cgacgaccug gacaaccugc      960

uggcccagau cggcgaccag uacgccgacc uguuucuggc cgccaagaac cuguccgacg     1020

ccauccugcu gagcgacauc cugagaguga acaccgagau caccaaggcc ccccugagcg     1080

ccucuaugau caagagauac gacgagcacc accaggaccu gacccugcug aaagcucucg     1140

ugcggcagca gcugccugag aaguacaaag agauuuucuu cgaccagagc aagaacggcu     1200

acgccggcua cauugacggc ggagccagcc aggaagaguu cuacaaguuc aucaagccca     1260

uccuggaaaa gauggacggc accgaggaac ugcucgugaa gcugaacaga gaggaccugc     1320

ugcggaagca gcggaccuuc gacaacggca gcauccccca ccagauccac cugggagagc     1380

ugcacgccau ucugcggcgg caggaagauu uuuacccauu ccugaaggac aaccgggaaa     1440

agaucgagaa gauccugacc uuccgcaucc ccuacuacgu gggcccucug gccaggggaa     1500

acagcagauu cgccuggaug accagaaaga gcgaggaaac caucaccccc uggaacuucg     1560

aggaaguggu ggacaagggc gcuuccgccc agagcuucau cgagcggaug accaacuucg     1620

auaagaaccu gcccaacgag aaggugcugc ccaagcacag ccugcuguac gaguacuuca     1680

ccguguauaa cgagcugacc aaagugaaau acgugaccga gggaaugaga aagcccgccu     1740

uccugagcgg cgagcagaaa aaggccaucg uggaccugcu guucaagacc aaccggaaag     1800

ugaccgugaa gcagcugaaa gaggacuacu ucaagaaaau cgagugcuuc gacuccgugg     1860

aaaucuccgg cguggaagau cgguucaacg ccucccuggg cacauaccac gaucugcuga     1920

aaauuaucaa ggacaaggac uuccuggaca augaggaaaa cgaggacauu cuggaagaua     1980

ucgugcugac ccugacacug uuugaggaca gagagaugau cgaggaacgg cugaaaaccu     2040

augcccaccu guucgacgac aaagugauga agcagcugaa gcggcggaga uacaccggcu     2100

ggggcaggcu gagccggaag cugaucaacg gcauccggga caagcagucc ggcaagacaa     2160

uccuggauuu ccugaagucc gacggcuucg ccaacagaaa cuucaugcag cugauccacg     2220

acgacagccu gaccuuuaaa gaggacaucc agaaagccca gguguccggc cagggcgaua     2280

gccugcacga gcacauugcc aaucuggccg gcagccccgc cauuaagaag ggcauccugc     2340

agacagugaa ggugguggac gagcucguga aagugauggg ccggcacaag cccgagaaca     2400

ucgugaucga aauggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg     2460

agagaaugaa gcggaucgaa gagggcauca aagagcuggg cagccagauc cugaaagaac     2520

accccgugga aaacacccag cugcagaacg agaagcugua ccuguacuac cugcagaaug     2580

ggcgggauau guacguggac caggaacugg acaucaaccg gcuguccgac uacgaugugg     2640

accauaucgu gccucagagc uuucugaagg acgacuccau cgacaacaag gugcugacca     2700

gaagcgacaa gaaccggggc aagagcgaca acgugcccuc cgaagagguc gugaagaaga     2760

ugaagaacua cuggcggcag cugcugaacg ccaagcugau uacccagaga aaguucgaca     2820

aucugaccaa ggccgagaga ggcggccuga gcgaacugga uaaggccggc uucaucaaga     2880

gacagcuggu ggaaacccgg cagaucacaa agcacguggc acagauccug gacucccgga     2940

ugaacacuaa guacgacgag aaugacaagc ugauccggga agugaaagug aucacccuga     3000

aguccaagcu gguguccgau uuccggaagg auuuccaguu uuacaaagug cgcgagauca     3060

acaacuacca ccacgcccac gacgccuacc ugaacgccgu cgugggaacc gcccugauca     3120

aaaaguaccc uaagcuggaa agcgaguucg uguacggcga cuacaaggug uacgacgugc     3180

ggaagaugau cgccaagagc gagcaggaaa ucggcaaggc uaccgccaag uacuucuucu     3240

acagcaacau caugaacuuu uucaagaccg agauuacccu ggccaacggc gagauccgga     3300

agcggccucu gaucgagaca aacggcgaaa ccggggagau cgugugggau aagggccggg     3360

auuuugccac cgugcggaaa gugcugagca ugccccaagu gaauaucgug aaaaagaccg     3420

aggugcagac aggcggcuuc agcaaagagu cuauccugcc caagaggaac agcgauaagc     3480

ugaucgccag aaagaaggac ugggacccua agaaguacgg cggcuucgac agccccaccg     3540

uggccuauuc ugugcuggug guggccaaag uggaaaaggg caaguccaag aaacugaaga     3600

gugugaaaga gcugcugggg aucaccauca uggaaagaag cagcuucgag aagaauccca     3660

ucgacuuucu ggaagccaag ggcuacaaag aagugaaaaa ggaccugauc aucaagcugc     3720

cuaaguacuc ccuguucgag cuggaaaacg gccggaagag aaugcuggcc ucugccggcg     3780

aacugcagaa gggaaacgaa cuggcccugc ccuccaaaua ugugaacuuc cuguaccugg     3840

ccagccacua ugagaagcug aagggcuccc ccgaggauaa ugagcagaaa cagcuguuug     3900

uggaacagca caagcacuac cuggacgaga ucaucgagca gaucagcgag uucuccaaga     3960

gagugauccu ggccgacgcu aaucuggaca aagugcuguc cgccuacaac aagcaccggg     4020

auaagcccau cagagagcag gccgagaaua ucauccaccu guuuacccug accaaucugg     4080

gagccccugc cgccuucaag uacuuugaca ccaccaucga ccggaagagg uacaccagca     4140

ccaaagaggu gcuggacgcc acccugaucc accagagcau caccggccug uacgagacac     4200

ggaucgaccu gucucagcug ggaggcgaca agcguccugc ugcuacuaag aaagcugguc     4260

aagcuaagaa aaagaaauaa ggacuagugu ccaccugucc cuccugggcu gcuggauugu     4320

cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4433


<210>  426
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS2

<400>  426

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala 
    


<210>  427
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS4

<400>  427

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  428
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  428

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  429
<211>  1629
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  429

Met Asn Phe Lys Ile Leu Pro Ile Ala Ile Asp Leu Gly Val Lys Asn 
1               5                   10                  15      


Thr Gly Val Phe Ser Ala Phe Tyr Gln Lys Gly Thr Ser Leu Glu Arg 
            20                  25                  30          


Leu Asp Asn Lys Asn Gly Lys Val Tyr Glu Leu Ser Lys Asp Ser Tyr 
        35                  40                  45              


Thr Leu Leu Met Asn Asn Arg Thr Ala Arg Arg His Gln Arg Arg Gly 
    50                  55                  60                  


Ile Asp Arg Lys Gln Leu Val Lys Arg Leu Phe Lys Leu Ile Trp Thr 
65                  70                  75                  80  


Glu Gln Leu Asn Leu Glu Trp Asp Lys Asp Thr Gln Gln Ala Ile Ser 
                85                  90                  95      


Phe Leu Phe Asn Arg Arg Gly Phe Ser Phe Ile Thr Asp Gly Tyr Ser 
            100                 105                 110         


Pro Glu Tyr Leu Asn Ile Val Pro Glu Gln Val Lys Ala Ile Leu Met 
        115                 120                 125             


Asp Ile Phe Asp Asp Tyr Asn Gly Glu Asp Asp Leu Asp Ser Tyr Leu 
    130                 135                 140                 


Lys Leu Ala Thr Glu Gln Glu Ser Lys Ile Ser Glu Ile Tyr Asn Lys 
145                 150                 155                 160 


Leu Met Gln Lys Ile Leu Glu Phe Lys Leu Met Lys Leu Cys Thr Asp 
                165                 170                 175     


Ile Lys Asp Asp Lys Val Ser Thr Lys Thr Leu Lys Glu Ile Thr Ser 
            180                 185                 190         


Tyr Glu Phe Glu Leu Leu Ala Asp Tyr Leu Ala Asn Tyr Ser Glu Ser 
        195                 200                 205             


Leu Lys Thr Gln Lys Phe Ser Tyr Thr Asp Lys Gln Gly Asn Leu Lys 
    210                 215                 220                 


Glu Leu Ser Tyr Tyr His His Asp Lys Tyr Asn Ile Gln Glu Phe Leu 
225                 230                 235                 240 


Lys Arg His Ala Thr Ile Asn Asp Arg Ile Leu Asp Thr Leu Leu Thr 
                245                 250                 255     


Asp Asp Leu Asp Ile Trp Asn Phe Asn Phe Glu Lys Phe Asp Phe Asp 
            260                 265                 270         


Lys Asn Glu Glu Lys Leu Gln Asn Gln Glu Asp Lys Asp His Ile Gln 
        275                 280                 285             


Ala His Leu His His Phe Val Phe Ala Val Asn Lys Ile Lys Ser Glu 
    290                 295                 300                 


Met Ala Ser Gly Gly Arg His Arg Ser Gln Tyr Phe Gln Glu Ile Thr 
305                 310                 315                 320 


Asn Val Leu Asp Glu Asn Asn His Gln Glu Gly Tyr Leu Lys Asn Phe 
                325                 330                 335     


Cys Glu Asn Leu His Asn Lys Lys Tyr Ser Asn Leu Ser Val Lys Asn 
            340                 345                 350         


Leu Val Asn Leu Ile Gly Asn Leu Ser Asn Leu Glu Leu Lys Pro Leu 
        355                 360                 365             


Arg Lys Tyr Phe Asn Asp Lys Ile His Ala Lys Ala Asp His Trp Asp 
    370                 375                 380                 


Glu Gln Lys Phe Thr Glu Thr Tyr Cys His Trp Ile Leu Gly Glu Trp 
385                 390                 395                 400 


Arg Val Gly Val Lys Asp Gln Asp Lys Lys Asp Gly Ala Lys Tyr Ser 
                405                 410                 415     


Tyr Lys Asp Leu Cys Asn Glu Leu Lys Gln Lys Val Thr Lys Ala Gly 
            420                 425                 430         


Leu Val Asp Phe Leu Leu Glu Leu Asp Pro Cys Arg Thr Ile Pro Pro 
        435                 440                 445             


Tyr Leu Asp Asn Asn Asn Arg Lys Pro Pro Lys Cys Gln Ser Leu Ile 
    450                 455                 460                 


Leu Asn Pro Lys Phe Leu Asp Asn Gln Tyr Pro Asn Trp Gln Gln Tyr 
465                 470                 475                 480 


Leu Gln Glu Leu Lys Lys Leu Gln Ser Ile Gln Asn Tyr Leu Asp Ser 
                485                 490                 495     


Phe Glu Thr Asp Leu Lys Val Leu Lys Ser Ser Lys Asp Gln Pro Tyr 
            500                 505                 510         


Phe Val Glu Tyr Lys Ser Ser Asn Gln Gln Ile Ala Ser Gly Gln Arg 
        515                 520                 525             


Asp Tyr Lys Asp Leu Asp Ala Arg Ile Leu Gln Phe Ile Phe Asp Arg 
    530                 535                 540                 


Val Lys Ala Ser Asp Glu Leu Leu Leu Asn Glu Ile Tyr Phe Gln Ala 
545                 550                 555                 560 


Lys Lys Leu Lys Gln Lys Ala Ser Ser Glu Leu Glu Lys Leu Glu Ser 
                565                 570                 575     


Ser Lys Lys Leu Asp Glu Val Ile Ala Asn Ser Gln Leu Ser Gln Ile 
            580                 585                 590         


Leu Lys Ser Gln His Thr Asn Gly Ile Phe Glu Gln Gly Thr Phe Leu 
        595                 600                 605             


His Leu Val Cys Lys Tyr Tyr Lys Gln Arg Gln Arg Ala Arg Asp Ser 
    610                 615                 620                 


Arg Leu Tyr Ile Met Pro Glu Tyr Arg Tyr Asp Lys Lys Leu His Lys 
625                 630                 635                 640 


Tyr Asn Asn Thr Gly Arg Phe Asp Asp Asp Asn Gln Leu Leu Thr Tyr 
                645                 650                 655     


Cys Asn His Lys Pro Arg Gln Lys Arg Tyr Gln Leu Leu Asn Asp Leu 
            660                 665                 670         


Ala Gly Val Leu Gln Val Ser Pro Asn Phe Leu Lys Asp Lys Ile Gly 
        675                 680                 685             


Ser Asp Asp Asp Leu Phe Ile Ser Lys Trp Leu Val Glu His Ile Arg 
    690                 695                 700                 


Gly Phe Lys Lys Ala Cys Glu Asp Ser Leu Lys Ile Gln Lys Asp Asn 
705                 710                 715                 720 


Arg Gly Leu Leu Asn His Lys Ile Asn Ile Ala Arg Asn Thr Lys Gly 
                725                 730                 735     


Lys Cys Glu Lys Glu Ile Phe Asn Leu Ile Cys Lys Ile Glu Gly Ser 
            740                 745                 750         


Glu Asp Lys Lys Gly Asn Tyr Lys His Gly Leu Ala Tyr Glu Leu Gly 
        755                 760                 765             


Val Leu Leu Phe Gly Glu Pro Asn Glu Ala Ser Lys Pro Glu Phe Asp 
    770                 775                 780                 


Arg Lys Ile Lys Lys Phe Asn Ser Ile Tyr Ser Phe Ala Gln Ile Gln 
785                 790                 795                 800 


Gln Ile Ala Phe Ala Glu Arg Lys Gly Asn Ala Asn Thr Cys Ala Val 
                805                 810                 815     


Cys Ser Ala Asp Asn Ala His Arg Met Gln Gln Ile Lys Ile Thr Glu 
            820                 825                 830         


Pro Val Glu Asp Asn Lys Asp Lys Ile Ile Leu Ser Ala Lys Ala Gln 
        835                 840                 845             


Arg Leu Pro Ala Ile Pro Thr Arg Ile Val Asp Gly Ala Val Lys Lys 
    850                 855                 860                 


Met Ala Thr Ile Leu Ala Lys Asn Ile Val Asp Asp Asn Trp Gln Asn 
865                 870                 875                 880 


Ile Lys Gln Val Leu Ser Ala Lys His Gln Leu His Ile Pro Ile Ile 
                885                 890                 895     


Thr Glu Ser Asn Ala Phe Glu Phe Glu Pro Ala Leu Ala Asp Val Lys 
            900                 905                 910         


Gly Lys Ser Leu Lys Asp Arg Arg Lys Lys Ala Leu Glu Arg Ile Ser 
        915                 920                 925             


Pro Glu Asn Ile Phe Lys Asp Lys Asn Asn Arg Ile Lys Glu Phe Ala 
    930                 935                 940                 


Lys Gly Ile Ser Ala Tyr Ser Gly Ala Asn Leu Thr Asp Gly Asp Phe 
945                 950                 955                 960 


Asp Gly Ala Lys Glu Glu Leu Asp His Ile Ile Pro Arg Ser His Lys 
                965                 970                 975     


Lys Tyr Gly Thr Leu Asn Asp Glu Ala Asn Leu Ile Cys Val Thr Arg 
            980                 985                 990         


Gly Asp Asn Lys Asn Lys Gly Asn  Arg Ile Phe Cys Leu  Arg Asp Leu 
        995                 1000                 1005             


Ala Asp  Asn Tyr Lys Leu Lys  Gln Phe Glu Thr Thr  Asp Asp Leu 
    1010                 1015                 1020             


Glu Ile  Glu Lys Lys Ile Ala  Asp Thr Ile Trp Asp  Ala Asn Lys 
    1025                 1030                 1035             


Lys Asp  Phe Lys Phe Gly Asn  Tyr Arg Ser Phe Ile  Asn Leu Thr 
    1040                 1045                 1050             


Pro Gln  Glu Gln Lys Ala Phe  Arg His Ala Leu Phe  Leu Ala Asp 
    1055                 1060                 1065             


Glu Asn  Pro Ile Lys Gln Ala  Val Ile Arg Ala Ile  Asn Asn Arg 
    1070                 1075                 1080             


Asn Arg  Thr Phe Val Asn Gly  Thr Gln Arg Tyr Phe  Ala Glu Val 
    1085                 1090                 1095             


Leu Ala  Asn Asn Ile Tyr Leu  Arg Ala Lys Lys Glu  Asn Leu Asn 
    1100                 1105                 1110             


Thr Asp  Lys Ile Ser Phe Asp  Tyr Phe Gly Ile Pro  Thr Ile Gly 
    1115                 1120                 1125             


Asn Gly  Arg Gly Ile Ala Glu  Ile Arg Gln Leu Tyr  Glu Lys Val 
    1130                 1135                 1140             


Asp Ser  Asp Ile Gln Ala Tyr  Ala Lys Gly Asp Lys  Pro Gln Ala 
    1145                 1150                 1155             


Ser Tyr  Ser His Leu Ile Asp  Ala Met Leu Ala Phe  Cys Ile Ala 
    1160                 1165                 1170             


Ala Asp  Glu His Arg Asn Asp  Gly Ser Ile Gly Leu  Glu Ile Asp 
    1175                 1180                 1185             


Lys Asn  Tyr Ser Leu Tyr Pro  Leu Asp Lys Asn Thr  Gly Glu Val 
    1190                 1195                 1200             


Phe Thr  Lys Asp Ile Phe Ser  Gln Ile Lys Ile Thr  Asp Asn Glu 
    1205                 1210                 1215             


Phe Ser  Asp Lys Lys Leu Val  Arg Lys Lys Ala Ile  Glu Gly Phe 
    1220                 1225                 1230             


Asn Thr  His Arg Gln Met Thr  Arg Asp Gly Ile Tyr  Ala Glu Asn 
    1235                 1240                 1245             


Tyr Leu  Pro Ile Leu Ile His  Lys Glu Leu Asn Glu  Val Arg Lys 
    1250                 1255                 1260             


Gly Tyr  Thr Trp Lys Asn Ser  Glu Glu Ile Lys Ile  Phe Lys Gly 
    1265                 1270                 1275             


Lys Lys  Tyr Asp Ile Gln Gln  Leu Asn Asn Leu Val  Tyr Cys Leu 
    1280                 1285                 1290             


Lys Phe  Val Asp Lys Pro Ile  Ser Ile Asp Ile Gln  Ile Ser Thr 
    1295                 1300                 1305             


Leu Glu  Glu Leu Arg Asn Ile  Leu Thr Thr Asn Asn  Ile Ala Ala 
    1310                 1315                 1320             


Thr Ala  Glu Tyr Tyr Tyr Ile  Asn Leu Lys Thr Gln  Lys Leu His 
    1325                 1330                 1335             


Glu Tyr  Tyr Ile Glu Asn Tyr  Asn Thr Ala Leu Gly  Tyr Lys Lys 
    1340                 1345                 1350             


Tyr Ser  Lys Glu Met Glu Phe  Leu Arg Ser Leu Ala  Tyr Arg Ser 
    1355                 1360                 1365             


Glu Arg  Val Lys Ile Lys Ser  Ile Asp Asp Val Lys  Gln Val Leu 
    1370                 1375                 1380             


Asp Lys  Asp Ser Asn Phe Ile  Ile Gly Lys Ile Thr  Leu Pro Phe 
    1385                 1390                 1395             


Lys Lys  Glu Trp Gln Arg Leu  Tyr Arg Glu Trp Gln  Asn Thr Thr 
    1400                 1405                 1410             


Ile Lys  Asp Asp Tyr Glu Phe  Leu Lys Ser Phe Phe  Asn Val Lys 
    1415                 1420                 1425             


Ser Ile  Thr Lys Leu His Lys  Lys Val Arg Lys Asp  Phe Ser Leu 
    1430                 1435                 1440             


Pro Ile  Ser Thr Asn Glu Gly  Lys Phe Leu Val Lys  Arg Lys Thr 
    1445                 1450                 1455             


Trp Asp  Asn Asn Phe Ile Tyr  Gln Ile Leu Asn Asp  Ser Asp Ser 
    1460                 1465                 1470             


Arg Ala  Asp Gly Thr Lys Pro  Phe Ile Pro Ala Phe  Asp Ile Ser 
    1475                 1480                 1485             


Lys Asn  Glu Ile Val Glu Ala  Ile Ile Asp Ser Phe  Thr Ser Lys 
    1490                 1495                 1500             


Asn Ile  Phe Trp Leu Pro Lys  Asn Ile Glu Leu Gln  Lys Val Asp 
    1505                 1510                 1515             


Asn Lys  Asn Ile Phe Ala Ile  Asp Thr Ser Lys Trp  Phe Glu Val 
    1520                 1525                 1530             


Glu Thr  Pro Ser Asp Leu Arg  Asp Ile Gly Ile Ala  Thr Ile Gln 
    1535                 1540                 1545             


Tyr Lys  Ile Asp Asn Asn Ser  Arg Pro Lys Val Arg  Val Lys Leu 
    1550                 1555                 1560             


Asp Tyr  Val Ile Asp Asp Asp  Ser Lys Ile Asn Tyr  Phe Met Asn 
    1565                 1570                 1575             


His Ser  Leu Leu Lys Ser Arg  Tyr Pro Asp Lys Val  Leu Glu Ile 
    1580                 1585                 1590             


Leu Lys  Gln Ser Thr Ile Ile  Glu Phe Glu Ser Ser  Gly Phe Asn 
    1595                 1600                 1605             


Lys Thr  Ile Lys Glu Met Leu  Gly Met Lys Leu Ala  Gly Ile Tyr 
    1610                 1615                 1620             


Asn Glu  Thr Ser Asn Asn 
    1625                 


<210>  430
<211>  1053
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  430

Met Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
                405                 410                 415     


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Ile Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Pro Pro Arg  Ile Ile Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Val Lys Ser Lys Lys  His Pro Gln Ile Ile  Lys Lys Gly 
    1040                 1045                 1050             


<210>  431
<211>  1409
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  431

Met Leu Phe Asn Lys Cys Ile Ile Ile Ser Ile Asn Leu Asp Phe Ser 
1               5                   10                  15      


Asn Lys Glu Lys Cys Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile 
            20                  25                  30          


Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Asn Tyr Lys Val 
        35                  40                  45              


Pro Ser Lys Lys Met Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile 
    50                  55                  60                  


Lys Lys Asn Leu Leu Gly Val Leu Leu Phe Asp Ser Gly Ile Thr Ala 
65                  70                  75                  80  


Glu Gly Arg Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg 
                85                  90                  95      


Arg Asn Arg Ile Leu Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala 
            100                 105                 110         


Thr Leu Asp Asp Ala Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val 
        115                 120                 125             


Pro Asp Asp Lys Arg Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val 
    130                 135                 140                 


Glu Glu Lys Val Tyr His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg 
145                 150                 155                 160 


Lys Tyr Leu Ala Asp Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr 
                165                 170                 175     


Leu Ala Leu Ala His Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu 
            180                 185                 190         


Gly Glu Phe Asn Ser Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp 
        195                 200                 205             


Phe Leu Asp Thr Tyr Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu 
    210                 215                 220                 


Asn Ser Lys Gln Leu Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu 
225                 230                 235                 240 


Glu Lys Lys Asp Arg Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser 
                245                 250                 255     


Gly Ile Phe Ser Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp 
            260                 265                 270         


Phe Arg Lys Cys Phe Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser 
        275                 280                 285             


Lys Glu Ser Tyr Asp Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly 
    290                 295                 300                 


Asp Asp Tyr Ser Asp Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala 
305                 310                 315                 320 


Ile Leu Leu Ser Gly Phe Leu Thr Val Thr Asp Asn Glu Thr Glu Ala 
                325                 330                 335     


Pro Leu Ser Ser Ala Met Ile Lys Arg Tyr Asn Glu His Lys Glu Asp 
            340                 345                 350         


Leu Ala Leu Leu Lys Glu Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr 
        355                 360                 365             


Asn Glu Val Phe Lys Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile 
    370                 375                 380                 


Asp Gly Lys Thr Asn Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu 
385                 390                 395                 400 


Leu Ala Glu Phe Glu Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg 
                405                 410                 415     


Glu Asp Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro 
            420                 425                 430         


Tyr Gln Ile His Leu Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala 
        435                 440                 445             


Lys Phe Tyr Pro Phe Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile 
    450                 455                 460                 


Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn 
465                 470                 475                 480 


Ser Asp Phe Ala Trp Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro 
                485                 490                 495     


Trp Asn Phe Glu Asp Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe 
            500                 505                 510         


Ile Asn Arg Met Thr Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val 
        515                 520                 525             


Leu Pro Lys His Ser Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu 
    530                 535                 540                 


Leu Thr Lys Val Arg Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe 
545                 550                 555                 560 


Leu Asp Ser Lys Gln Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp 
                565                 570                 575     


Lys Arg Lys Val Thr Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile 
            580                 585                 590         


Tyr Gly Tyr Asp Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn 
        595                 600                 605             


Ser Ser Leu Ser Thr Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys 
    610                 615                 620                 


Glu Phe Leu Asp Asp Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile 
625                 630                 635                 640 


His Thr Leu Thr Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu 
                645                 650                 655     


Ser Lys Phe Glu Asn Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser 
            660                 665                 670         


Arg Arg His Tyr Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn 
        675                 680                 685             


Gly Ile Arg Asp Glu Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile 
    690                 695                 700                 


Asp Asp Gly Ile Ser Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp 
705                 710                 715                 720 


Ala Leu Ser Phe Lys Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp 
                725                 730                 735     


Glu Asp Lys Gly Asn Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser 
            740                 745                 750         


Pro Ala Ile Lys Lys Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu 
        755                 760                 765             


Leu Val Lys Val Met Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu 
    770                 775                 780                 


Met Ala Arg Glu Asn Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln 
785                 790                 795                 800 


Gln Arg Leu Lys Arg Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys 
                805                 810                 815     


Ile Leu Lys Glu Asn Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn 
            820                 825                 830         


Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys 
        835                 840                 845             


Asp Met Tyr Thr Gly Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr 
    850                 855                 860                 


Asp Ile Asp His Ile Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile 
865                 870                 875                 880 


Asp Asn Lys Val Leu Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp 
                885                 890                 895     


Asp Phe Pro Ser Leu Glu Val Val Lys Lys Arg Lys Thr Phe Trp Tyr 
            900                 905                 910         


Gln Leu Leu Lys Ser Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu 
        915                 920                 925             


Thr Lys Ala Glu Arg Gly Gly Leu Leu Pro Glu Asp Lys Ala Gly Phe 
    930                 935                 940                 


Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala 
945                 950                 955                 960 


Arg Leu Leu Asp Glu Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg 
                965                 970                 975     


Ala Val Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Thr Leu Val Ser 
            980                 985                 990         


Gln Phe Arg Lys Asp Phe Glu Leu  Tyr Lys Val Arg Glu  Ile Asn Asp 
        995                 1000                 1005             


Phe His  His Ala His Asp Ala  Tyr Leu Asn Ala Val  Ile Ala Ser 
    1010                 1015                 1020             


Ala Leu  Leu Lys Lys Tyr Pro  Lys Leu Glu Pro Glu  Phe Val Tyr 
    1025                 1030                 1035             


Gly Asp  Tyr Pro Lys Tyr Asn  Ser Phe Arg Glu Arg  Lys Ser Ala 
    1040                 1045                 1050             


Thr Glu  Lys Val Tyr Phe Tyr  Ser Asn Ile Met Asn  Ile Phe Lys 
    1055                 1060                 1065             


Lys Ser  Ile Ser Leu Ala Asp  Gly Arg Val Ile Glu  Arg Pro Leu 
    1070                 1075                 1080             


Ile Glu  Val Asn Glu Glu Thr  Gly Glu Ser Val Trp  Asn Lys Glu 
    1085                 1090                 1095             


Ser Asp  Leu Ala Thr Val Arg  Arg Val Leu Ser Tyr  Pro Gln Val 
    1100                 1105                 1110             


Asn Val  Val Lys Lys Val Glu  Glu Gln Asn His Gly  Leu Asp Arg 
    1115                 1120                 1125             


Gly Lys  Pro Lys Gly Leu Phe  Asn Ala Asn Leu Ser  Ser Lys Pro 
    1130                 1135                 1140             


Lys Pro  Asn Ser Asn Glu Asn  Leu Val Gly Ala Lys  Glu Tyr Leu 
    1145                 1150                 1155             


Asp Pro  Lys Lys Tyr Gly Gly  Tyr Ala Gly Ile Ser  Asn Ser Phe 
    1160                 1165                 1170             


Ala Val  Leu Val Lys Gly Thr  Ile Glu Lys Gly Ala  Lys Lys Lys 
    1175                 1180                 1185             


Ile Thr  Asn Val Leu Glu Phe  Gln Gly Ile Ser Ile  Leu Asp Arg 
    1190                 1195                 1200             


Ile Asn  Tyr Arg Lys Asp Lys  Leu Asn Phe Leu Leu  Glu Lys Gly 
    1205                 1210                 1215             


Tyr Lys  Asp Ile Glu Leu Ile  Ile Glu Leu Pro Lys  Tyr Ser Leu 
    1220                 1225                 1230             


Phe Glu  Leu Ser Asp Gly Ser  Arg Arg Met Leu Ala  Ser Ile Leu 
    1235                 1240                 1245             


Ser Thr  Asn Asn Lys Arg Gly  Glu Ile His Lys Gly  Asn Gln Ile 
    1250                 1255                 1260             


Phe Leu  Ser Gln Lys Phe Val  Lys Leu Leu Tyr His  Ala Lys Arg 
    1265                 1270                 1275             


Ile Ser  Asn Thr Ile Asn Glu  Asn His Arg Lys Tyr  Val Glu Asn 
    1280                 1285                 1290             


His Lys  Lys Glu Phe Glu Glu  Leu Phe Tyr Tyr Ile  Leu Glu Phe 
    1295                 1300                 1305             


Asn Glu  Asn Tyr Val Gly Ala  Lys Lys Asn Gly Lys  Leu Leu Asn 
    1310                 1315                 1320             


Ser Ala  Phe Gln Ser Trp Gln  Asn His Ser Ile Asp  Glu Leu Cys 
    1325                 1330                 1335             


Ser Ser  Phe Ile Gly Pro Thr  Gly Ser Glu Arg Lys  Gly Leu Phe 
    1340                 1345                 1350             


Glu Leu  Thr Ser Arg Gly Ser  Ala Ala Asp Phe Glu  Phe Leu Gly 
    1355                 1360                 1365             


Val Lys  Ile Pro Arg Tyr Arg  Asp Tyr Thr Pro Ser  Ser Leu Leu 
    1370                 1375                 1380             


Lys Asp  Ala Thr Leu Ile His  Gln Ser Val Thr Gly  Leu Tyr Glu 
    1385                 1390                 1395             


Thr Arg  Ile Asp Leu Ala Lys  Leu Gly Glu Gly 
    1400                 1405                 


<210>  432
<211>  1101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  432

Met Trp Tyr Ala Ser Leu Met Ser Ala His His Leu Arg Val Gly Ile 
1               5                   10                  15      


Asp Val Gly Thr His Ser Val Gly Leu Ala Thr Leu Arg Val Asp Asp 
            20                  25                  30          


His Gly Thr Pro Ile Glu Leu Leu Ser Ala Leu Ser His Ile His Asp 
        35                  40                  45              


Ser Gly Val Gly Lys Glu Gly Lys Lys Asp His Asp Thr Arg Lys Lys 
    50                  55                  60                  


Leu Ser Gly Ile Ala Arg Arg Ala Arg Arg Leu Leu His His Arg Arg 
65                  70                  75                  80  


Thr Gln Leu Gln Gln Leu Asp Glu Val Leu Arg Asp Leu Gly Phe Pro 
                85                  90                  95      


Ile Pro Thr Pro Gly Glu Phe Leu Asp Leu Asn Glu Gln Thr Asp Pro 
            100                 105                 110         


Tyr Arg Val Trp Arg Val Arg Ala Arg Leu Val Glu Glu Lys Leu Pro 
        115                 120                 125             


Glu Glu Leu Arg Gly Pro Ala Ile Ser Met Ala Val Arg His Ile Ala 
    130                 135                 140                 


Arg His Arg Gly Trp Arg Asn Pro Tyr Ser Lys Val Glu Ser Leu Leu 
145                 150                 155                 160 


Ser Pro Ala Glu Glu Ser Pro Phe Met Lys Ala Leu Arg Glu Arg Ile 
                165                 170                 175     


Leu Ala Thr Thr Gly Glu Val Leu Asp Asp Gly Ile Thr Pro Gly Gln 
            180                 185                 190         


Ala Met Ala Gln Val Ala Leu Thr His Asn Ile Ser Met Arg Gly Pro 
        195                 200                 205             


Glu Gly Ile Leu Gly Lys Leu His Gln Ser Asp Asn Ala Asn Glu Ile 
    210                 215                 220                 


Arg Lys Ile Cys Ala Arg Gln Gly Val Ser Pro Asp Val Cys Lys Gln 
225                 230                 235                 240 


Leu Leu Arg Ala Val Phe Lys Ala Asp Ser Pro Arg Gly Ser Ala Val 
                245                 250                 255     


Ser Arg Val Ala Pro Asp Pro Leu Pro Gly Gln Gly Ser Phe Arg Arg 
            260                 265                 270         


Ala Pro Lys Cys Asp Pro Glu Phe Gln Arg Phe Arg Ile Ile Ser Ile 
        275                 280                 285             


Val Ala Asn Leu Arg Ile Ser Glu Thr Lys Gly Glu Asn Arg Pro Leu 
    290                 295                 300                 


Thr Ala Asp Glu Arg Arg His Val Val Thr Phe Leu Thr Glu Asp Ser 
305                 310                 315                 320 


Gln Ala Asp Leu Thr Trp Val Asp Val Ala Glu Lys Leu Gly Val His 
                325                 330                 335     


Arg Arg Asp Leu Arg Gly Thr Ala Val His Thr Asp Asp Gly Glu Arg 
            340                 345                 350         


Ser Ala Ala Arg Pro Pro Ile Asp Ala Thr Asp Arg Ile Met Arg Gln 
        355                 360                 365             


Thr Lys Ile Ser Ser Leu Lys Thr Trp Trp Glu Glu Ala Asp Ser Glu 
    370                 375                 380                 


Gln Arg Gly Ala Met Ile Arg Tyr Leu Tyr Glu Asp Pro Thr Asp Ser 
385                 390                 395                 400 


Glu Cys Ala Glu Ile Ile Ala Glu Leu Pro Glu Glu Asp Gln Ala Lys 
                405                 410                 415     


Leu Asp Ser Leu His Leu Pro Ala Gly Arg Ala Ala Tyr Ser Arg Glu 
            420                 425                 430         


Ser Leu Thr Ala Leu Ser Asp His Met Leu Ala Thr Thr Asp Asp Leu 
        435                 440                 445             


His Glu Ala Arg Lys Arg Leu Phe Gly Val Asp Asp Ser Trp Ala Pro 
    450                 455                 460                 


Pro Ala Glu Ala Ile Asn Ala Pro Val Gly Asn Pro Ser Val Asp Arg 
465                 470                 475                 480 


Thr Leu Lys Ile Val Gly Arg Tyr Leu Ser Ala Val Glu Ser Met Trp 
                485                 490                 495     


Gly Thr Pro Glu Val Ile His Val Glu His Val Arg Asp Gly Phe Thr 
            500                 505                 510         


Ser Glu Arg Met Ala Asp Glu Arg Asp Lys Ala Asn Arg Arg Arg Tyr 
        515                 520                 525             


Asn Asp Asn Gln Glu Ala Met Lys Lys Ile Gln Arg Asp Tyr Gly Lys 
    530                 535                 540                 


Glu Gly Tyr Ile Ser Arg Gly Asp Ile Val Arg Leu Asp Ala Leu Glu 
545                 550                 555                 560 


Leu Gln Gly Cys Ala Cys Leu Tyr Cys Gly Thr Thr Ile Gly Tyr His 
                565                 570                 575     


Thr Cys Gln Leu Asp His Ile Val Pro Gln Ala Gly Pro Gly Ser Asn 
            580                 585                 590         


Asn Arg Arg Gly Asn Leu Val Ala Val Cys Glu Arg Cys Asn Arg Ser 
        595                 600                 605             


Lys Ser Asn Thr Pro Phe Ala Val Trp Ala Gln Lys Cys Gly Ile Pro 
    610                 615                 620                 


His Val Gly Val Lys Glu Ala Ile Gly Arg Val Arg Gly Trp Arg Lys 
625                 630                 635                 640 


Gln Thr Pro Asn Thr Ser Ser Glu Asp Leu Thr Arg Leu Lys Lys Glu 
                645                 650                 655     


Val Ile Ala Arg Leu Arg Arg Thr Gln Glu Asp Pro Glu Ile Asp Glu 
            660                 665                 670         


Arg Ser Met Glu Ser Val Ala Trp Met Ala Asn Glu Leu His His Arg 
        675                 680                 685             


Ile Ala Ala Ala Tyr Pro Glu Thr Thr Val Met Val Tyr Arg Gly Ser 
    690                 695                 700                 


Ile Thr Ala Ala Ala Arg Lys Ala Ala Gly Ile Asp Ser Arg Ile Asn 
705                 710                 715                 720 


Leu Ile Gly Glu Lys Gly Arg Lys Asp Arg Ile Asp Arg Arg His His 
                725                 730                 735     


Ala Val Asp Ala Ser Val Val Ala Leu Met Glu Ala Ser Val Ala Lys 
            740                 745                 750         


Thr Leu Ala Glu Arg Ser Ser Leu Arg Gly Glu Gln Arg Leu Thr Gly 
        755                 760                 765             


Lys Glu Gln Thr Trp Lys Gln Tyr Thr Gly Ser Thr Val Gly Ala Arg 
    770                 775                 780                 


Glu His Phe Glu Met Trp Arg Gly His Met Leu His Leu Thr Glu Leu 
785                 790                 795                 800 


Phe Asn Glu Arg Leu Ala Glu Asp Lys Val Tyr Val Thr Gln Asn Ile 
                805                 810                 815     


Arg Leu Arg Leu Ser Asp Gly Asn Ala His Thr Val Asn Pro Ser Lys 
            820                 825                 830         


Leu Val Ser His Arg Leu Gly Asp Gly Leu Thr Val Gln Gln Ile Asp 
        835                 840                 845             


Arg Ala Cys Thr Pro Ala Leu Trp Cys Ala Leu Thr Arg Glu Lys Asp 
    850                 855                 860                 


Phe Asp Glu Lys Asn Gly Leu Pro Ala Arg Glu Asp Arg Ala Ile Arg 
865                 870                 875                 880 


Val His Gly His Glu Ile Lys Ser Ser Asp Tyr Ile Gln Val Phe Ser 
                885                 890                 895     


Lys Arg Lys Lys Thr Asp Ser Asp Arg Asp Glu Thr Pro Phe Gly Ala 
            900                 905                 910         


Ile Ala Val Arg Gly Gly Phe Val Glu Ile Gly Pro Ser Ile His His 
        915                 920                 925             


Ala Arg Ile Tyr Arg Val Glu Gly Lys Lys Pro Val Tyr Ala Met Leu 
    930                 935                 940                 


Arg Val Phe Thr His Asp Leu Leu Ser Gln Arg His Gly Asp Leu Phe 
945                 950                 955                 960 


Ser Ala Val Ile Pro Pro Gln Ser Ile Ser Met Arg Cys Ala Glu Pro 
                965                 970                 975     


Lys Leu Arg Lys Ala Ile Thr Thr Gly Asn Ala Thr Tyr Leu Gly Trp 
            980                 985                 990         


Val Val Val Gly Asp Glu Leu Glu  Ile Asn Val Asp Ser  Phe Thr Lys 
        995                 1000                 1005             


Tyr Ala  Ile Gly Arg Phe Leu  Glu Asp Phe Pro Asn  Thr Thr Arg 
    1010                 1015                 1020             


Trp Arg  Ile Cys Gly Tyr Asp  Thr Asn Ser Lys Leu  Thr Leu Lys 
    1025                 1030                 1035             


Pro Ile  Val Leu Ala Ala Glu  Gly Leu Glu Asn Pro  Ser Ser Ala 
    1040                 1045                 1050             


Val Asn  Glu Ile Val Glu Leu  Lys Gly Trp Arg Val  Ala Ile Asn 
    1055                 1060                 1065             


Val Leu  Thr Lys Val His Pro  Thr Val Val Arg Arg  Asp Ala Leu 
    1070                 1075                 1080             


Gly Arg  Pro Arg Tyr Ser Ser  Arg Ser Asn Leu Pro  Thr Ser Trp 
    1085                 1090                 1095             


Thr Ile  Glu 
    1100     


<210>  433
<211>  1388
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  433

Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Thr Asp Asn Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Val Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         


Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             


His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 


Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         


Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             


Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu Glu Lys Lys Asp Arg 
    210                 215                 220                 


Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Arg Lys Cys Phe 
                245                 250                 255     


Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             


Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Phe Leu Thr Val Thr Asp Asn Glu Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asn Glu His Lys Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     


Glu Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         


Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             


Gln Glu Asp Phe Tyr Val Tyr Leu Lys Lys Leu Leu Ala Glu Phe Glu 
    370                 375                 380                 


Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 


Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 


Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             


Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 


Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 


Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Tyr Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             


Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
625                 630                 635                 640 


Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         


Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 


Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Glu Asp Lys Gly Asn 
705                 710                 715                 720 


Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg 
    770                 775                 780                 


Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn 
785                 790                 795                 800 


Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp 
                805                 810                 815     


Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly 
            820                 825                 830         


Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile 
        835                 840                 845             


Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile Asp Asn Lys Val Leu 
    850                 855                 860                 


Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu 
865                 870                 875                 880 


Glu Val Val Lys Lys Arg Lys Thr Phe Trp Tyr Gln Leu Leu Lys Ser 
                885                 890                 895     


Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
            900                 905                 910         


Gly Gly Leu Ser Pro Glu Asp Lys Ala Gly Phe Ile Gln Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu 
    930                 935                 940                 


Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg Ala Val Arg Thr Val 
945                 950                 955                 960 


Lys Ile Ile Thr Leu Lys Ser Thr Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Phe Glu Leu Tyr Lys Val Arg Glu Ile Asn Asp Phe His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Ser Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Lys  Leu Glu Pro Glu Phe  Val Tyr Gly Asp Tyr  Pro Lys Tyr 
    1010                 1015                 1020             


Asn Ser  Phe Arg Glu Arg Lys  Ser Ala Thr Glu Lys  Val Tyr Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Ile  Phe Lys Lys Ser Ile  Ser Leu Ala 
    1040                 1045                 1050             


Asp Gly  Arg Val Ile Glu Arg  Pro Leu Ile Glu Val  Asn Glu Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ser Val Trp Asn  Lys Glu Ser Asp Leu  Ala Thr Val 
    1070                 1075                 1080             


Arg Arg  Val Leu Ser Tyr Pro  Gln Val Asn Val Val  Lys Lys Val 
    1085                 1090                 1095             


Glu Glu  Gln Asn His Gly Leu  Asp Arg Gly Lys Pro  Lys Gly Leu 
    1100                 1105                 1110             


Phe Asn  Ala Asn Leu Ser Ser  Lys Pro Lys Pro Asn  Ser Asn Glu 
    1115                 1120                 1125             


Asn Leu  Val Gly Ala Lys Glu  Tyr Leu Asp Pro Lys  Lys Tyr Gly 
    1130                 1135                 1140             


Gly Tyr  Ala Gly Ile Ser Asn  Ser Phe Thr Val Leu  Val Lys Gly 
    1145                 1150                 1155             


Thr Ile  Glu Lys Gly Ala Lys  Lys Lys Ile Thr Asn  Val Leu Glu 
    1160                 1165                 1170             


Phe Gln  Gly Ile Ser Ile Leu  Asp Arg Ile Asn Tyr  Arg Lys Asp 
    1175                 1180                 1185             


Lys Leu  Asn Phe Leu Leu Glu  Lys Gly Tyr Lys Asp  Ile Glu Leu 
    1190                 1195                 1200             


Ile Ile  Glu Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Ser Asp Gly 
    1205                 1210                 1215             


Ser Arg  Arg Met Leu Ala Ser  Ile Leu Ser Thr Asn  Asn Lys Arg 
    1220                 1225                 1230             


Gly Glu  Ile His Lys Gly Asn  Gln Ile Phe Leu Ser  Gln Lys Phe 
    1235                 1240                 1245             


Val Lys  Leu Leu Tyr His Ala  Lys Arg Ile Ser Asn  Thr Ile Asn 
    1250                 1255                 1260             


Glu Asn  His Arg Lys Tyr Val  Glu Asn His Lys Lys  Glu Phe Glu 
    1265                 1270                 1275             


Glu Leu  Phe Tyr Tyr Ile Leu  Glu Phe Asn Glu Asn  Tyr Val Gly 
    1280                 1285                 1290             


Ala Lys  Lys Asn Gly Lys Leu  Leu Asn Ser Ala Phe  Gln Ser Trp 
    1295                 1300                 1305             


Gln Asn  His Ser Ile Asp Glu  Leu Cys Ser Ser Phe  Ile Gly Pro 
    1310                 1315                 1320             


Thr Gly  Ser Glu Arg Lys Gly  Leu Phe Glu Leu Thr  Ser Arg Gly 
    1325                 1330                 1335             


Ser Ala  Ala Asp Phe Glu Phe  Leu Gly Val Lys Ile  Pro Arg Tyr 
    1340                 1345                 1350             


Arg Asp  Tyr Thr Pro Ser Ser  Leu Leu Lys Asp Ala  Thr Leu Ile 
    1355                 1360                 1365             


His Gln  Ser Val Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ala 
    1370                 1375                 1380             


Lys Leu  Gly Glu Gly 
    1385             


<210>  434
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  434

Met Ala Ala Phe Lys Pro Asn Ser Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Thr Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asn 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Gly Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Ser Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Leu Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  435
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  435

Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Ile Ala Gly Asp Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  


Ala Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Gly Ile Phe Ala Glu Glu Met Ser Lys Thr Asp Ala Asn 
                85                  90                  95      


Phe Phe Cys Arg Leu Ser Asp Ser Phe Tyr Val Asp Asn Glu Lys Arg 
            100                 105                 110         


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Glu Tyr 
        115                 120                 125             


His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     


Gln Asn Thr Ser Val Asp Gly Ile Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Ala Ser Gly Ile Glu Asp Gly Ser Leu Lys Lys Leu 
        195                 200                 205             


Glu Asp Asn Lys Asp Val Ala Lys Ile Leu Val Glu Lys Val Thr Arg 
    210                 215                 220                 


Lys Glu Lys Leu Glu Arg Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 


Ala Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Ser Lys Gly 
                245                 250                 255     


Asn Phe Gln Lys Pro Phe Asp Leu Ile Glu Lys Ser Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ser Leu Leu Ala Leu Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Ala Tyr Ser 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Ala Glu Thr Glu Thr Asn 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Thr His Glu Glu 
                325                 330                 335     


Asp Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys His 
            340                 345                 350         


Tyr Glu Glu Ile Phe Ser Asn Thr Glu Lys His Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Lys Gln Ala Asp Phe Tyr Lys Tyr Met Lys Met 
    370                 375                 380                 


Thr Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu 
385                 390                 395                 400 


Lys Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Lys Glu Asn Tyr Asp Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly 
    450                 455                 460                 


Gln Ser Glu Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Leu Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Val Arg Tyr Ile Asn Asp Gln Gly Lys Thr Ser Tyr 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Glu Gln Ile Phe Asn Asp Leu Phe Lys Gln 
545                 550                 555                 560 


Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Arg Asn Met 
                565                 570                 575     


Ser His Val Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         


Ser Ser Tyr Ser Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln 
        595                 600                 605             


Glu Ile Leu Asp Asn Pro Val Asn Thr Glu Met Leu Glu Asn Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Gln Gln Phe Ser Asp Val Leu Asp Gly Val Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Met 
            660                 665                 670         


Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Thr Thr Ala 
705                 710                 715                 720 


Asp Lys Asp Ile Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Ser Val Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr 
    770                 775                 780                 


Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 


Gly Asn Arg Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Glu Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Tyr 
    930                 935                 940                 


Glu Lys Asp Asp His Gly Asn Thr Met Lys Gln Val Arg Ile Val Thr 
945                 950                 955                 960 


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Asp Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Ala Gln Lys Asp  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Asp Thr Val Lys  Lys Val Met 
    1055                 1060                 1065             


Ser Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Lys Ala Thr  Ile Lys Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Thr  Asn Trp Asp Pro Met  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Leu  Asp Ser Pro Asn Met  Ala Tyr Ala Val Val  Ile Glu Tyr 
    1115                 1120                 1125             


Ala Lys  Gly Lys Asn Lys Leu  Val Phe Glu Lys Lys  Ile Ile Arg 
    1130                 1135                 1140             


Val Thr  Ile Met Glu Arg Lys  Ala Phe Glu Lys Asp  Glu Lys Ala 
    1145                 1150                 1155             


Phe Leu  Glu Glu Gln Gly Tyr  Arg Gln Pro Lys Val  Leu Ala Lys 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Glu Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Ala Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Gln Val 
    1190                 1195                 1200             


Leu Pro  Asn His Leu Val Thr  Leu Leu His His Ala  Ala Asn Cys 
    1205                 1210                 1215             


Glu Val  Ser Asp Gly Lys Ser  Leu Asp Tyr Ile Glu  Ser Asn Arg 
    1220                 1225                 1230             


Glu Met  Phe Ala Glu Leu Leu  Ala His Val Ser Glu  Phe Ala Lys 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Glu Ala  Asn Leu Asn Lys Ile  Asn Gln Leu 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Glu Gly  Asp Ile Lys Ala Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asp Leu Met Ala Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Phe Phe Glu Thr Thr  Ile Glu Arg Lys Arg  Tyr Asn Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Asn Ser  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ser Arg Lys  Arg Leu Asp Asp 
    1325                 1330                 


<210>  436
<211>  1345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  436

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Ser His Ile Glu Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Gly Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Thr Glu Asp Lys Arg 
            100                 105                 110         


Gly Glu Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Lys Tyr 
        115                 120                 125             


His Glu Asn Phe Pro Thr Ile Tyr His Leu Arg Gln Tyr Leu Ala Asp 
    130                 135                 140                 


Asn Pro Glu Lys Val Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Lys Phe Asp Thr 
                165                 170                 175     


Arg Asn Asn Asp Val Gln Arg Leu Phe Gln Glu Phe Leu Ala Val Tyr 
            180                 185                 190         


Asp Asn Thr Phe Glu Asn Ser Ser Leu Gln Glu Gln Asn Val Gln Val 
        195                 200                 205             


Glu Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 


Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Arg Phe Ala Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     


Glu Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu 
        275                 280                 285             


Leu Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Val Gly Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys 
                325                 330                 335     


Gln Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         


Asp Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             


Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu 
    370                 375                 380                 


Gly Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Asp Asn Gln Asp Arg Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp 
    450                 455                 460                 


Leu Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 


Ile Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Asn Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Lys Thr Glu Gln Gly Lys Thr Ala Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr Lys 
545                 550                 555                 560 


Asp Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     


Val Asp Leu Thr Gly Leu Asp Lys Glu Asn Lys Val Phe Asn Ala Ser 
            580                 585                 590         


Tyr Gly Thr Tyr His Asp Leu Cys Lys Ile Leu Asp Lys Asp Phe Leu 
        595                 600                 605             


Asp Asn Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Asn Tyr 
625                 630                 635                 640 


Ser Asp Leu Leu Thr Lys Glu Gln Val Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
        675                 680                 685             


Asn Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser 
    690                 695                 700                 


Phe Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn 
705                 710                 715                 720 


Leu Asn Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met 
            740                 745                 750         


Gly His Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu 
    770                 775                 780                 


Thr Asp Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr 
            820                 825                 830         


Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             


Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asp Val Pro Ser Lys Asp Val Val Arg Lys Met Lys 
865                 870                 875                 880 


Ser Tyr Trp Ser Lys Leu Leu Ser Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Thr Asp 
    930                 935                 940                 


Glu Asn Asn Lys Lys Ile Arg Gln Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 


Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Ile Gly Lys Ala Leu Leu Gly Val  Tyr Pro Gln Leu Glu  Pro Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Pro His  Phe His Gly His Lys  Glu Asn Lys 
    1010                 1015                 1020             


Ala Thr  Ala Lys Lys Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe 
    1025                 1030                 1035             


Lys Lys  Asp Asp Val Arg Thr  Asp Lys Asn Gly Glu  Ile Ile Trp 
    1040                 1045                 1050             


Lys Lys  Asp Glu His Ile Ser  Asn Ile Lys Lys Val  Leu Ser Tyr 
    1055                 1060                 1065             


Pro Gln  Val Asn Ile Val Lys  Lys Val Glu Glu Gln  Thr Gly Gly 
    1070                 1075                 1080             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly Asn Ser  Asp Lys Leu 
    1085                 1090                 1095             


Ile Pro  Arg Lys Thr Lys Lys  Phe Tyr Trp Asp Thr  Lys Lys Tyr 
    1100                 1105                 1110             


Gly Gly  Phe Asp Ser Pro Ile  Val Ala Tyr Ser Ile  Leu Val Ile 
    1115                 1120                 1125             


Ala Asp  Ile Glu Lys Gly Lys  Ser Lys Lys Leu Lys  Thr Val Lys 
    1130                 1135                 1140             


Ala Leu  Val Gly Val Thr Ile  Met Glu Lys Met Thr  Phe Glu Arg 
    1145                 1150                 1155             


Asp Pro  Val Ala Phe Leu Glu  Arg Lys Gly Tyr Arg  Asn Val Gln 
    1160                 1165                 1170             


Glu Glu  Asn Ile Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Lys Leu 
    1175                 1180                 1185             


Glu Asn  Gly Arg Lys Arg Leu  Leu Ala Ser Ala Arg  Glu Leu Gln 
    1190                 1195                 1200             


Lys Gly  Asn Glu Ile Val Leu  Pro Asn His Leu Gly  Thr Leu Leu 
    1205                 1210                 1215             


Tyr His  Ala Lys Asn Ile His  Lys Val Asp Glu Pro  Lys His Leu 
    1220                 1225                 1230             


Asp Tyr  Val Asp Lys His Lys  Asp Glu Phe Lys Glu  Leu Leu Asp 
    1235                 1240                 1245             


Val Val  Ser Asn Phe Ser Lys  Lys Tyr Thr Leu Ala  Glu Gly Asn 
    1250                 1255                 1260             


Leu Glu  Lys Ile Lys Glu Leu  Tyr Ala Gln Asn Asn  Gly Glu Asp 
    1265                 1270                 1275             


Leu Lys  Glu Leu Ala Ser Ser  Phe Ile Asn Leu Leu  Thr Phe Thr 
    1280                 1285                 1290             


Ala Ile  Gly Ala Pro Ala Thr  Phe Lys Phe Phe Asp  Lys Asn Ile 
    1295                 1300                 1305             


Asp Arg  Lys Arg Tyr Thr Ser  Thr Thr Glu Ile Leu  Asn Ala Thr 
    1310                 1315                 1320             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1325                 1330                 1335             


Leu Asn  Lys Leu Gly Gly Asp  
    1340                 1345 


<210>  437
<211>  1056
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  437

Met Gln Thr Thr Asn Leu Ser Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ala Val Val Glu Ile Asn Glu Asn Glu Asp Pro 
            20                  25                  30          


Ile Gly Leu Ile Asp Val Gly Val Arg Ile Phe Glu Arg Ala Glu Val 
        35                  40                  45              


Pro Lys Thr Gly Glu Ser Leu Ala Leu Ser Arg Arg Leu Ala Arg Ser 
    50                  55                  60                  


Thr Arg Arg Leu Ile Arg Arg Arg Ala His Arg Leu Leu Leu Ala Lys 
65                  70                  75                  80  


Arg Phe Leu Lys Arg Glu Gly Ile Leu Ser Thr Ile Asp Leu Glu Lys 
                85                  90                  95      


Gly Leu Pro Asn Gln Ala Trp Glu Leu Arg Val Ala Gly Leu Glu Arg 
            100                 105                 110         


Arg Leu Ser Ala Ile Glu Trp Gly Ala Val Leu Leu His Leu Ile Lys 
        115                 120                 125             


His Arg Gly Tyr Leu Ser Lys Arg Lys Asn Glu Ser Gln Thr Asn Asn 
    130                 135                 140                 


Lys Glu Leu Gly Ala Leu Leu Ser Gly Val Ala Gln Asn His Gln Leu 
145                 150                 155                 160 


Leu Gln Ser Asp Asp Tyr Arg Thr Pro Ala Glu Leu Ala Leu Lys Lys 
                165                 170                 175     


Phe Ala Lys Glu Glu Gly His Ile Arg Asn Gln Arg Gly Ala Tyr Thr 
            180                 185                 190         


His Thr Phe Asn Arg Leu Asp Leu Leu Ala Glu Leu Asn Leu Leu Phe 
        195                 200                 205             


Ala Gln Gln His Gln Phe Gly Asn Pro His Cys Lys Glu His Ile Gln 
    210                 215                 220                 


Gln Tyr Met Thr Glu Leu Leu Met Trp Gln Lys Pro Ala Leu Ser Gly 
225                 230                 235                 240 


Glu Ala Ile Leu Lys Met Leu Gly Lys Cys Thr His Glu Lys Asn Glu 
                245                 250                 255     


Phe Lys Ala Ala Lys His Thr Tyr Ser Ala Glu Arg Phe Val Trp Leu 
            260                 265                 270         


Thr Lys Leu Asn Asn Leu Arg Ile Leu Glu Asp Gly Ala Glu Arg Ala 
        275                 280                 285             


Leu Asn Glu Glu Glu Arg Gln Leu Leu Ile Asn His Pro Tyr Glu Lys 
    290                 295                 300                 


Ser Lys Leu Thr Tyr Ala Gln Val Arg Lys Leu Leu Gly Leu Ser Glu 
305                 310                 315                 320 


Gln Ala Ile Phe Lys His Leu Arg Tyr Ser Lys Glu Asn Ala Glu Ser 
                325                 330                 335     


Ala Thr Phe Met Glu Leu Lys Ala Trp His Ala Ile Arg Lys Ala Leu 
            340                 345                 350         


Glu Asn Gln Gly Leu Lys Asp Thr Trp Gln Asp Leu Ala Lys Lys Pro 
        355                 360                 365             


Asp Leu Leu Asp Glu Ile Gly Thr Ala Phe Ser Leu Tyr Lys Thr Asp 
    370                 375                 380                 


Glu Asp Ile Gln Gln Tyr Leu Thr Asn Lys Val Pro Asn Ser Val Ile 
385                 390                 395                 400 


Asn Ala Leu Leu Val Ser Leu Asn Phe Asp Lys Phe Ile Glu Leu Ser 
                405                 410                 415     


Leu Lys Ser Leu Arg Lys Ile Leu Pro Leu Met Glu Gln Gly Lys Arg 
            420                 425                 430         


Tyr Asp Gln Ala Cys Arg Glu Ile Tyr Gly His His Tyr Gly Glu Ala 
        435                 440                 445             


Asn Gln Lys Thr Ser Gln Leu Leu Pro Ala Ile Pro Ala Gln Glu Ile 
    450                 455                 460                 


Arg Asn Pro Val Val Leu Arg Thr Leu Ser Gln Ala Arg Lys Val Ile 
465                 470                 475                 480 


Asn Ala Ile Ile Arg Gln Tyr Gly Ser Pro Ala Arg Val His Ile Glu 
                485                 490                 495     


Thr Gly Arg Glu Leu Gly Lys Ser Phe Lys Glu Arg Arg Glu Ile Gln 
            500                 505                 510         


Lys Gln Gln Glu Asp Asn Arg Thr Lys Arg Glu Ser Ala Val Gln Lys 
        515                 520                 525             


Phe Lys Glu Leu Phe Ser Asp Phe Ser Ser Glu Pro Lys Ser Lys Asp 
    530                 535                 540                 


Ile Leu Lys Phe Arg Leu Tyr Glu Gln Gln His Gly Lys Cys Leu Tyr 
545                 550                 555                 560 


Ser Gly Lys Glu Ile Asn Ile His Arg Leu Asn Glu Lys Gly Tyr Val 
                565                 570                 575     


Glu Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe 
            580                 585                 590         


Asn Asn Lys Val Leu Val Leu Ala Ser Glu Asn Gln Asn Lys Gly Asn 
        595                 600                 605             


Gln Thr Pro Tyr Glu Trp Leu Gln Gly Lys Ile Asn Ser Glu Arg Trp 
    610                 615                 620                 


Lys Asn Phe Val Ala Leu Val Leu Gly Ser Gln Cys Ser Ala Ala Lys 
625                 630                 635                 640 


Lys Gln Arg Leu Leu Thr Gln Val Ile Asp Asp Asn Lys Phe Ile Asp 
                645                 650                 655     


Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ala Arg Phe Leu Ser Asn Tyr 
            660                 665                 670         


Ile Gln Glu Asn Leu Leu Leu Val Gly Lys Asn Lys Lys Asn Val Phe 
        675                 680                 685             


Thr Pro Asn Gly Gln Ile Thr Ala Leu Leu Arg Ser Arg Trp Gly Leu 
    690                 695                 700                 


Ile Lys Ala Arg Glu Asn Asn Asn Arg His His Ala Leu Asp Ala Ile 
705                 710                 715                 720 


Val Val Ala Cys Ala Thr Pro Ser Met Gln Gln Lys Ile Thr Arg Phe 
                725                 730                 735     


Ile Arg Phe Lys Glu Val His Pro Tyr Lys Ile Glu Asn Arg Tyr Glu 
            740                 745                 750         


Met Val Asp Gln Glu Ser Gly Glu Ile Ile Ser Pro His Phe Pro Glu 
        755                 760                 765             


Pro Trp Ala Tyr Phe Arg Gln Glu Val Asn Ile Arg Val Phe Asp Asn 
    770                 775                 780                 


His Pro Asp Thr Val Leu Lys Glu Met Leu Pro Asp Arg Pro Gln Ala 
785                 790                 795                 800 


Asn His Gln Phe Val Gln Pro Leu Phe Val Ser Arg Ala Pro Thr Arg 
                805                 810                 815     


Lys Met Ser Gly Gln Gly His Met Glu Thr Ile Lys Ser Ala Lys Arg 
            820                 825                 830         


Leu Ala Glu Gly Ile Ser Val Leu Arg Ile Pro Leu Thr Gln Leu Lys 
        835                 840                 845             


Pro Asn Leu Leu Glu Asn Met Val Asn Lys Glu Arg Glu Pro Ala Leu 
    850                 855                 860                 


Tyr Ala Gly Leu Lys Ala Arg Leu Ala Glu Phe Asn Gln Asp Pro Ala 
865                 870                 875                 880 


Lys Ala Phe Ala Thr Pro Phe Tyr Lys Gln Gly Gly Gln Gln Val Lys 
                885                 890                 895     


Ala Ile Arg Val Glu Gln Val Gln Lys Ser Gly Val Leu Val Arg Glu 
            900                 905                 910         


Asn Asn Gly Val Ala Asp Asn Ala Ser Ile Val Arg Thr Asp Val Phe 
        915                 920                 925             


Ile Lys Asn Asn Lys Phe Phe Leu Val Pro Ile Tyr Thr Trp Gln Val 
    930                 935                 940                 


Ala Lys Gly Ile Leu Pro Asn Lys Ala Ile Val Ala His Lys Asn Glu 
945                 950                 955                 960 


Asp Glu Trp Glu Glu Met Asp Glu Gly Ala Lys Phe Lys Phe Ser Leu 
                965                 970                 975     


Phe Pro Asn Asp Leu Val Glu Leu Lys Thr Lys Lys Glu Tyr Phe Phe 
            980                 985                 990         


Gly Tyr Tyr Ile Gly Leu Asp Arg  Ala Thr Gly Asn Ile  Ser Leu Lys 
        995                 1000                 1005             


Glu His  Asp Gly Glu Ile Ser  Lys Gly Lys Asp Gly  Val Tyr Arg 
    1010                 1015                 1020             


Val Gly  Val Lys Leu Ala Leu  Ser Phe Glu Lys Tyr  Gln Val Asp 
    1025                 1030                 1035             


Glu Leu  Gly Lys Asn Arg Gln  Ile Cys Arg Pro Gln  Gln Arg Gln 
    1040                 1045                 1050             


Pro Val  Arg 
    1055     


<210>  438
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  438

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  439
<211>  1084
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  439

Met Lys Tyr His Val Gly Ile Asp Val Gly Thr Phe Ser Val Gly Leu 
1               5                   10                  15      


Ala Ala Ile Glu Val Asp Asp Ala Gly Met Pro Ile Lys Thr Leu Ser 
            20                  25                  30          


Leu Val Ser His Ile His Asp Ser Gly Leu Asp Pro Asp Glu Ile Lys 
        35                  40                  45              


Ser Ala Val Thr Arg Leu Ala Ser Ser Gly Ile Ala Arg Arg Thr Arg 
    50                  55                  60                  


Arg Leu Tyr Arg Arg Lys Arg Arg Arg Leu Gln Gln Leu Asp Lys Phe 
65                  70                  75                  80  


Ile Gln Arg Gln Gly Trp Pro Val Ile Glu Leu Glu Asp Tyr Ser Asp 
                85                  90                  95      


Pro Leu Tyr Pro Trp Lys Val Arg Ala Glu Leu Ala Ala Ser Tyr Ile 
            100                 105                 110         


Ala Asp Glu Lys Glu Arg Gly Glu Lys Leu Ser Val Ala Leu Arg His 
        115                 120                 125             


Ile Ala Arg His Arg Gly Trp Arg Asn Pro Tyr Ala Lys Val Ser Ser 
    130                 135                 140                 


Leu Tyr Leu Pro Asp Gly Pro Ser Asp Ala Phe Lys Ala Ile Arg Glu 
145                 150                 155                 160 


Glu Ile Lys Arg Ala Ser Gly Gln Pro Val Pro Glu Thr Ala Thr Val 
                165                 170                 175     


Gly Gln Met Val Thr Leu Cys Glu Leu Gly Thr Leu Lys Leu Arg Gly 
            180                 185                 190         


Glu Gly Gly Val Leu Ser Ala Arg Leu Gln Gln Ser Asp Tyr Ala Arg 
        195                 200                 205             


Glu Ile Gln Glu Ile Cys Arg Met Gln Glu Ile Gly Gln Glu Leu Tyr 
    210                 215                 220                 


Arg Lys Ile Ile Asp Val Val Phe Ala Ala Glu Ser Pro Lys Gly Ser 
225                 230                 235                 240 


Ala Ser Ser Arg Val Gly Lys Asp Pro Leu Gln Pro Gly Lys Asn Arg 
                245                 250                 255     


Ala Leu Lys Ala Ser Asp Ala Phe Gln Arg Tyr Arg Ile Ala Ala Leu 
            260                 265                 270         


Ile Gly Asn Leu Arg Val Arg Val Asp Gly Glu Lys Arg Ile Leu Ser 
        275                 280                 285             


Val Glu Glu Lys Asn Leu Val Phe Asp His Leu Val Asn Leu Thr Pro 
    290                 295                 300                 


Lys Lys Glu Pro Glu Trp Val Thr Ile Ala Glu Ile Leu Gly Ile Asp 
305                 310                 315                 320 


Arg Gly Gln Leu Ile Gly Thr Ala Thr Met Thr Asp Asp Gly Glu Arg 
                325                 330                 335     


Ala Gly Ala Arg Pro Pro Thr His Asp Thr Asn Arg Ser Ile Val Asn 
            340                 345                 350         


Ser Arg Ile Ala Pro Leu Val Asp Trp Trp Lys Thr Ala Ser Ala Leu 
        355                 360                 365             


Glu Gln His Ala Met Val Lys Ala Leu Ser Asn Ala Glu Val Asp Asp 
    370                 375                 380                 


Phe Asp Ser Pro Glu Gly Ala Lys Val Gln Ala Phe Phe Ala Asp Leu 
385                 390                 395                 400 


Asp Asp Asp Val His Ala Lys Leu Asp Ser Leu His Leu Pro Val Gly 
                405                 410                 415     


Arg Ala Ala Tyr Ser Glu Asp Thr Leu Val Arg Leu Thr Arg Arg Met 
            420                 425                 430         


Leu Ser Asp Gly Val Asp Leu Tyr Thr Ala Arg Leu Gln Glu Phe Gly 
        435                 440                 445             


Ile Glu Pro Ser Trp Thr Pro Pro Thr Pro Arg Ile Gly Glu Pro Val 
    450                 455                 460                 


Gly Asn Pro Ala Val Asp Arg Val Leu Lys Thr Val Ser Arg Trp Leu 
465                 470                 475                 480 


Glu Ser Ala Thr Lys Thr Trp Gly Ala Pro Glu Arg Val Ile Ile Glu 
                485                 490                 495     


His Val Arg Glu Gly Phe Val Thr Glu Lys Arg Ala Arg Glu Met Asp 
            500                 505                 510         


Gly Asp Met Arg Arg Arg Ala Ala Arg Asn Ala Lys Leu Phe Gln Glu 
        515                 520                 525             


Met Gln Glu Lys Leu Asn Val Gln Gly Lys Pro Ser Arg Ala Asp Leu 
    530                 535                 540                 


Trp Arg Tyr Gln Ser Val Gln Arg Gln Asn Cys Gln Cys Ala Tyr Cys 
545                 550                 555                 560 


Gly Ser Pro Ile Thr Phe Ser Asn Ser Glu Met Asp His Ile Val Pro 
                565                 570                 575     


Arg Ala Gly Gln Gly Ser Thr Asn Thr Arg Glu Asn Leu Val Ala Val 
            580                 585                 590         


Cys His Arg Cys Asn Gln Ser Lys Gly Asn Thr Pro Phe Ala Ile Trp 
        595                 600                 605             


Ala Lys Asn Thr Ser Ile Glu Gly Val Ser Val Lys Glu Ala Val Glu 
    610                 615                 620                 


Arg Thr Arg His Trp Val Thr Asp Thr Gly Met Arg Ser Thr Asp Phe 
625                 630                 635                 640 


Lys Lys Phe Thr Lys Ala Val Val Glu Arg Phe Gln Arg Ala Thr Met 
                645                 650                 655     


Asp Glu Glu Ile Asp Ala Arg Ser Met Glu Ser Val Ala Trp Met Ala 
            660                 665                 670         


Asn Glu Leu Arg Ser Arg Val Ala Gln His Phe Ala Ser His Gly Thr 
        675                 680                 685             


Thr Val Arg Val Tyr Arg Gly Ser Leu Thr Ala Glu Ala Arg Arg Ala 
    690                 695                 700                 


Ser Gly Ile Ser Gly Lys Leu Lys Phe Phe Asp Gly Val Gly Lys Ser 
705                 710                 715                 720 


Arg Leu Asp Arg Arg His His Ala Ile Asp Ala Ala Val Ile Ala Phe 
                725                 730                 735     


Thr Ser Asp Tyr Val Ala Glu Thr Leu Ala Val Arg Ser Asn Leu Lys 
            740                 745                 750         


Gln Ser Gln Ala His Arg Gln Glu Ala Pro Gln Trp Arg Glu Phe Thr 
        755                 760                 765             


Gly Lys Asp Ala Glu His Arg Ala Ala Trp Arg Val Trp Cys Gln Lys 
    770                 775                 780                 


Met Glu Lys Leu Ser Ala Leu Leu Thr Glu Asp Leu Arg Asp Asp Arg 
785                 790                 795                 800 


Val Val Val Met Ser Asn Val Arg Leu Arg Leu Gly Asn Gly Ser Ala 
                805                 810                 815     


His Lys Glu Thr Ile Gly Lys Leu Ser Lys Val Lys Leu Ser Ser Gln 
            820                 825                 830         


Leu Ser Val Ser Asp Ile Asp Lys Ala Ser Ser Glu Ala Leu Trp Cys 
        835                 840                 845             


Ala Leu Thr Arg Glu Pro Gly Phe Asp Pro Lys Glu Gly Leu Pro Ala 
    850                 855                 860                 


Asn Pro Glu Arg His Ile Arg Val Asn Gly Thr His Val Tyr Ala Gly 
865                 870                 875                 880 


Asp Asn Ile Gly Leu Phe Pro Val Ser Ala Gly Ser Ile Ala Leu Arg 
                885                 890                 895     


Gly Gly Tyr Ala Glu Leu Gly Ser Ser Phe His His Ala Arg Val Tyr 
            900                 905                 910         


Lys Ile Thr Ser Gly Lys Lys Pro Ala Phe Ala Met Leu Arg Val Tyr 
        915                 920                 925             


Thr Ile Asp Leu Leu Pro Tyr Arg Asn Gln Asp Leu Phe Ser Val Glu 
    930                 935                 940                 


Leu Lys Pro Gln Thr Met Ser Met Arg Gln Ala Glu Lys Lys Leu Arg 
945                 950                 955                 960 


Asp Ala Leu Ala Thr Gly Asn Ala Glu Tyr Leu Gly Trp Leu Val Val 
                965                 970                 975     


Asp Asp Glu Leu Val Val Asp Thr Ser Lys Ile Ala Thr Asp Gln Val 
            980                 985                 990         


Lys Ala Val Glu Ala Glu Leu Gly  Thr Ile Arg Arg Trp  Arg Val Asp 
        995                 1000                 1005             


Gly Phe  Phe Ser Pro Ser Lys  Leu Arg Leu Arg Pro  Leu Gln Met 
    1010                 1015                 1020             


Ser Lys  Glu Gly Ile Lys Lys  Glu Ser Ala Pro Glu  Leu Ser Lys 
    1025                 1030                 1035             


Ile Ile  Asp Arg Pro Gly Trp  Leu Pro Ala Val Asn  Lys Leu Phe 
    1040                 1045                 1050             


Ser Asp  Gly Asn Val Thr Val  Val Arg Arg Asp Ser  Leu Gly Arg 
    1055                 1060                 1065             


Val Arg  Leu Glu Ser Thr Ala  His Leu Pro Val Thr  Trp Lys Val 
    1070                 1075                 1080             


Gln 
    


<210>  440
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  440

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Val Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Val Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asn Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  441
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  441

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Ala Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn Arg Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Arg Thr Ser Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Phe Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
    290                 295                 300                 


Ile Asn Tyr Val Lys Asn Glu Lys Ala Met Gly Pro Ala Lys Leu Phe 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
385                 390                 395                 400 


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
                405                 410                 415     


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
            420                 425                 430         


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
                485                 490                 495     


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
        515                 520                 525             


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Ser 
            580                 585                 590         


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Phe Asp Val Arg Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
    690                 695                 700                 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Asn 
            740                 745                 750         


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Asp Gln 
        755                 760                 765             


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
    770                 775                 780                 


Glu Ser Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
785                 790                 795                 800 


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
                805                 810                 815     


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
            820                 825                 830         


Ala Lys Val Gly Lys Asp Lys Ala Asp Glu Thr Tyr Val Leu Gly Lys 
        835                 840                 845             


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
865                 870                 875                 880 


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
                885                 890                 895     


Gln Ile Asn Glu Lys Gly Lys Glu Val Pro Cys Asn Pro Phe Leu Lys 
            900                 905                 910         


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
        915                 920                 925             


Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Lys Asp Ser Asn Asn Lys Val Val Leu Gln 
945                 950                 955                 960 


Ser Val Ser Pro Trp Arg Ala Asp Val Tyr Phe Asn Lys Thr Thr Gly 
                965                 970                 975     


Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Thr Tyr Lys Ile Ser  Gln Glu Lys Tyr Asn  Asp Ile Lys 
        995                 1000                 1005             


Lys Lys  Glu Gly Val Asp Ser  Asp Ser Glu Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Asn Asp Leu Leu Leu  Val Lys Asp Thr Glu  Thr Lys Glu 
    1025                 1030                 1035             


Gln Gln  Leu Phe Arg Phe Leu  Ser Arg Thr Met Pro  Lys Gln Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Asp Lys Gln Lys  Phe Glu Gly 
    1055                 1060                 1065             


Gly Glu  Ala Leu Ile Lys Val  Leu Gly Asn Val Ala  Asn Ser Gly 
    1070                 1075                 1080             


Gln Cys  Lys Lys Gly Leu Gly  Lys Ser Asn Ile Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Val Arg  Thr Asp Val Leu Gly  Asn Gln His Ile Ile  Lys Asn Glu 
    1100                 1105                 1110             


Gly Asp  Lys Pro Lys Leu Asp  Phe 
    1115                 1120     


<210>  442
<211>  851
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  442

Met Thr Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Val Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Glu Ala Lys Thr Gly Lys Val Ile His Ala Asn 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ala Arg Arg Leu Thr Arg Arg Lys Lys His Arg Val 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Asp Ile Leu Thr Asp Phe 
65                  70                  75                  80  


Arg Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Ser Asp Tyr Ala Lys Ser Ile Asp Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Ser Met Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Lys Asn Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ser Asn Tyr Asn Lys Gln Ile Thr Asp 
        195                 200                 205             


Glu Phe Ile Glu Asp Tyr Ile Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Ser Phe Tyr Pro Glu Glu Tyr Arg Ala Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Phe Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Pro Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys Glu Tyr Leu Val 
    290                 295                 300                 


Asn Phe Ala Lys Asn Ser Lys Thr Leu Gly Ala Ser Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Leu Val Asp Gly Asp Val Lys Asp Ile Ser Gly Tyr 
                325                 330                 335     


Arg Glu Asp Ser Lys Gly Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Thr Thr Val Asp Ile Asp Asn Leu Ser 
        355                 360                 365             


Arg Asp Ile Leu Asp Lys Leu Ala Asp Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Ala Ile Asn Gln Asn Leu Pro Glu Gln Phe 
385                 390                 395                 400 


Thr Lys Glu Gln Ile Ser Glu Ile Val Gln Ile Arg Lys Ser Gln Ser 
                405                 410                 415     


Ser Ala Phe Asn Lys Gly Trp His Ser Phe Ser Ala Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Ala Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Ala Thr Lys Lys Ser Ser Lys Asn 
    450                 455                 460                 


Thr Lys Thr Ile Asp Glu Lys Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Thr Ile Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Asp Asp Glu Lys Lys Phe Ile Asp Lys Lys Asn Lys 
        515                 520                 525             


Glu Asn Lys Lys Glu Lys Asp Asp Ser Leu Lys Arg Ala Ala Tyr Leu 
    530                 535                 540                 


Tyr Asn Gly Thr Asp Lys Leu Pro Asp Asp Val Phe His Gly Asn Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Pro Ile Leu Ile Gln Asp Leu Val His Asn Ser 
            580                 585                 590         


Asn Asn Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Trp Ala Asn Gln Glu 
    610                 615                 620                 


Lys Glu Gln Lys Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Ala Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Asp Tyr Val Leu Lys Gln Lys Gly Ile 
                645                 650                 655     


Gly Lys Lys Lys Arg Glu Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Leu Gln Thr Ala Leu Lys Glu 
    690                 695                 700                 


Leu Gly Lys Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Lys 
            740                 745                 750         


Leu Trp Gln Lys His Glu Asn Leu Met Phe Glu Asn Tyr Gly Glu Asn 
        755                 760                 765             


Gln Val Val Asn Lys Glu Thr Gly Glu Ile Leu Ser Ile Ser Asp Asp 
    770                 775                 780                 


Glu Tyr Lys Glu Ser Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Lys Ala Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Phe Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Leu Gly Lys Asp Lys Lys Glu Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys 
    850     


<210>  443
<211>  865
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  443

Met Arg His Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Leu Asp Ile Pro Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Lys 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Leu Ile Arg Glu Gly Ile Leu 
65                  70                  75                  80  


Thr Lys Glu Glu Leu Asp Lys Leu Phe Glu Glu Lys His Glu Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ser Glu Arg Asn Asn Lys Glu Asn Ser Thr Met Leu 
    130                 135                 140                 


Lys His Ile Glu Glu Asn Arg Ala Leu Leu Ser Gly Tyr Arg Thr Val 
145                 150                 155                 160 


Ala Glu Met Ile Val Lys Asp Pro Lys Phe Ala Phe His Lys Arg Asn 
                165                 170                 175     


Lys Gly Glu Asn Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu Glu Arg 
            180                 185                 190         


Glu Ile Lys Leu Ile Phe Thr Lys Gln Arg Glu Tyr Gly Asn Ile Val 
        195                 200                 205             


Cys Thr Glu Thr Phe Glu Asn Glu Tyr Ile Met Ile Trp Ala Ser Gln 
    210                 215                 220                 


Arg Pro Phe Ala Ser Lys Asn Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Val Ala Trp Glu His Ile Asn Lys Leu Arg Leu Val Tyr 
            260                 265                 270         


Pro Thr Gly Thr Arg Gly Leu Thr Asp Glu Glu Arg Arg Leu Leu Tyr 
        275                 280                 285             


Lys Gln Ala Phe His Lys Asn Lys Ile Thr Tyr His Asp Ile Arg Thr 
    290                 295                 300                 


Leu Leu His Leu Pro Asp Asp Thr Tyr Phe Lys Gly Ile Val Tyr Asp 
305                 310                 315                 320 


Arg Gly Ala Pro Leu Lys Gln Ser Glu Thr Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ala Tyr His Gln Ile Arg Lys Ala Val Asp Lys Val Tyr Gly Lys 
            340                 345                 350         


Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Leu Phe Lys Asp Asp Ala Asp Ile Arg Ser Tyr Leu Arg 
    370                 375                 380                 


Asn Glu Tyr Glu Gln Asn Gly Lys Arg Met Pro Asn Leu Ala Asn Lys 
385                 390                 395                 400 


Val Tyr Asp Asn Glu Pro Ile Glu Glu Leu Leu Asn Leu Ser Phe Thr 
                405                 410                 415     


Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg Asn Ile Leu Pro Tyr 
            420                 425                 430         


Met Glu Gln Gly Glu Val Tyr Ser Val Ala Cys Glu Arg Ala Gly Tyr 
        435                 440                 445             


Thr Phe Thr Gly Pro Lys Lys Lys Gln Lys Thr Leu Leu Leu Pro Asn 
    450                 455                 460                 


Ile Pro Pro Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val Ser 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp Glu Arg 
            500                 505                 510         


Arg Lys Thr Lys Arg Glu Gln Asp Glu Asn Arg Lys Lys Asn Glu Thr 
        515                 520                 525             


Ala Ile Arg Gln Leu Met Glu Tyr Gly Leu Thr Ile Asn Pro Thr Gly 
    530                 535                 540                 


His Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly Arg Cys 
545                 550                 555                 560 


Ala Tyr Ser Leu Gln Pro Ile Glu Ile Glu Arg Leu Leu Glu Pro Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Lys Glu Asn Arg Glu Lys 
        595                 600                 605             


Gly Asn Arg Ile Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu Arg Trp 
    610                 615                 620                 


Gln Gln Phe Lys Thr Phe Val Leu Thr Asn Lys Gln Phe Ser Lys Lys 
625                 630                 635                 640 


Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu Glu Ala 
                645                 650                 655     


Glu Trp Lys Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Arg Phe 
            660                 665                 670         


Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Glu Ser Asp Asp 
        675                 680                 685             


Lys Gln Lys Val Tyr Thr Val Asn Gly Arg Val Thr Ala His Leu Arg 
    690                 695                 700                 


Ser Arg Trp Asp Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His His 
705                 710                 715                 720 


Ala Val Asp Ala Ala Ile Val Ala Cys Thr Ser Pro Ser Asp Ile Ala 
                725                 730                 735     


Arg Val Thr Ala Phe Tyr Gln Arg Arg Glu Gln Cys Lys Glu Ser Ala 
            740                 745                 750         


Lys Lys Ala Glu Pro His Phe Pro Gln Pro Trp Pro His Phe Ala Asp 
        755                 760                 765             


Glu Leu Arg Ala Arg Leu Ser Lys Asn Pro Lys Glu Ser Ile Lys Ala 
    770                 775                 780                 


Leu Asn Leu Gly Asn Tyr Asp Asp Gln Lys Leu Glu Ser Leu Gln Pro 
785                 790                 795                 800 


Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala His 
                805                 810                 815     


Gln Glu Thr Leu Arg Arg Tyr Ile Gly Ile Asp Glu Arg Ser Gly Lys 
            820                 825                 830         


Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu Asp Glu 
        835                 840                 845             


Thr Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Pro Ala Ser 
    850                 855                 860                 


Ala 
865 


<210>  444
<211>  961
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  444

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Asn Gly Asp Glu Glu Lys Gly Lys 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Val Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Ile Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Thr Gln Ser Phe Leu Lys Tyr Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Gln Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Ser Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Gly Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Leu Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Lys Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Asn Val Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Glu Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Lys Gly Ser Lys 
            660                 665                 670         


Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser Ala Leu Arg His 
        675                 680                 685             


Thr Trp Gly Phe Ser Thr Lys Asp Arg Asn Asn His Leu His His Ala 
    690                 695                 700                 


Ile Asp Ala Ala Ile Ile Ala Tyr Ala Asn Asn Ser Ile Val Lys Asp 
705                 710                 715                 720 


Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser Ala Glu Leu Tyr 
                725                 730                 735     


Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys Arg Lys Phe Phe 
            740                 745                 750         


Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp Lys Ile Asp Glu 
        755                 760                 765             


Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser Gly Ala Leu His 
    770                 775                 780                 


Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln Ser Tyr Gly Gly 
785                 790                 795                 800 


Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys Ile Arg Lys Val 
                805                 810                 815     


Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg Val Asp Ile Phe 
            820                 825                 830         


Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro Ile Tyr Thr Met 
        835                 840                 845             


Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val Ala Arg Ser Lys 
    850                 855                 860                 


Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu Asn Tyr Glu Phe 
865                 870                 875                 880 


Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile Gln Thr Lys Asp 
                885                 890                 895     


Met Gln Glu Pro Glu Leu Val Tyr Phe Asn Ala Phe Thr Ser Ser Thr 
            900                 905                 910         


Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe Glu Thr Leu Ser 
        915                 920                 925             


Lys Asn Leu Lys Val Phe Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu 
    930                 935                 940                 


Val Thr Lys Ala Glu Phe Arg Gln Arg Glu Asp Phe Lys Asn Glu Leu 
945                 950                 955                 960 


Arg 
    


<210>  445
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  445

Met Ala Lys Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Ile Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ile Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Asn Gly Asp Glu Glu Lys Gly Lys 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Ile Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Leu Tyr Thr Lys Asp Asp Leu 
        275                 280                 285             


Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu Thr Tyr Lys 
    290                 295                 300                 


Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu Phe Lys Gly 
305                 310                 315                 320 


Glu Lys Gly Asn Tyr Phe Ile Glu Phe Lys Lys Tyr Lys Glu Phe Ile 
                325                 330                 335     


Lys Ala Leu Gly Glu His Ser Leu Ser Gln Asp Asp Leu Asn Glu Ile 
            340                 345                 350         


Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu Lys Lys Ala 
        355                 360                 365             


Leu Ala Lys Tyr Asp Leu Asn Glu Asn Gln Ile Asp Asn Leu Ser Lys 
    370                 375                 380                 


Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala Leu Lys Leu 
385                 390                 395                 400 


Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu Ala Cys Asn 
                405                 410                 415     


Glu Leu Thr Leu Lys Val Ala Ile Asn Glu Asp Lys Lys Asp Phe Leu 
            420                 425                 430         


Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr Asn Pro Val 
        435                 440                 445             


Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn Ala Leu Leu 
    450                 455                 460                 


Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu Ala Arg Glu 
465                 470                 475                 480 


Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys Glu Gln Asn 
                485                 490                 495     


Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys Glu Lys Leu 
            500                 505                 510         


Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg Leu Phe Lys 
        515                 520                 525             


Glu Gln Lys Glu Phe Cys Val Tyr Ser Gly Glu Lys Ile Lys Leu Ser 
    530                 535                 540                 


Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile Tyr Pro Tyr 
545                 550                 555                 560 


Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu Val Phe Thr 
                565                 570                 575     


Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu Ala Phe Gly 
            580                 585                 590         


Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala Lys Asn Leu 
        595                 600                 605             


Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr Lys Asp Lys 
    610                 615                 620                 


Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr Arg Tyr Ile 
625                 630                 635                 640 


Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp Phe Leu Pro 
                645                 650                 655     


Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln Lys Gly Ser 
            660                 665                 670         


Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser Ala Leu Arg 
        675                 680                 685             


His Thr Trp Gly Phe Ser Thr Lys Asp Arg Asn Asn His Leu His His 
    690                 695                 700                 


Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser Ile Val Lys 
705                 710                 715                 720 


Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Thr Ala Glu Leu 
                725                 730                 735     


Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys Arg Lys Phe 
            740                 745                 750         


Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp Lys Ile Asp 
        755                 760                 765             


Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser Gly Ala Leu 
    770                 775                 780                 


His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln Ser Tyr Gly 
785                 790                 795                 800 


Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys Ile Arg Lys 
                805                 810                 815     


Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg Val Asp Ile 
            820                 825                 830         


Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro Ile Tyr Thr 
        835                 840                 845             


Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val Ala Arg Ser 
    850                 855                 860                 


Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu Asn Tyr Glu 
865                 870                 875                 880 


Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile Gln Thr Lys 
                885                 890                 895     


Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe Thr Ser Ser 
            900                 905                 910         


Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe Glu Thr Leu 
        915                 920                 925             


Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu Lys Glu Val 
    930                 935                 940                 


Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe Glu Lys Tyr 
945                 950                 955                 960 


Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe Arg Gln Arg 
                965                 970                 975     


Glu Glu Phe Lys Asn Glu Leu Arg 
            980                 


<210>  446
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  446

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn Arg Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Phe Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn Tyr Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr His 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Thr Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu Arg His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  447
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  447

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Val Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Thr Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Lys Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Thr Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  448
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  448

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Asp His Ser Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Ile Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Ile Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Leu Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Lys Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Thr Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Glu Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Ala Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Val Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Leu Val Tyr Phe Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  449
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  449

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Val Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Lys Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Thr Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  450
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  450

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Asp His Ser Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn Tyr Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Phe Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  451
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  451

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Phe Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Ile Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asn Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  452
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  452

Met Val Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Val Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Lys Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Thr Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  453
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  453

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Asp His Ser Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn Tyr Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  454
<211>  985
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  454

Met Ala Lys Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Val Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Thr Leu Ile Ser Pro Tyr Glu Leu Arg Phe Lys Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Leu Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asn Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Val Lys Glu Asn Glu Glu Lys Leu Ala Asn Tyr Lys 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Ile Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Gln Lys Gln Arg Glu Phe Gly Phe Asn Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Val Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Glu Phe Val Ala Leu Thr Arg Ile Val 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Asn Lys Asn Asn Ser Tyr Ile Lys Asp Asp 
        275                 280                 285             


Leu Asn Ala Leu Leu Ser Glu Val Leu Lys Asn Gly Thr Leu Thr Tyr 
    290                 295                 300                 


Lys Gln Thr Lys Lys Ile Leu Gly Leu Ser Asp Asp Tyr Glu Phe Lys 
305                 310                 315                 320 


Gly Gln Lys Gly Asn Tyr Phe Ile Glu Phe Lys Lys Tyr Lys Glu Phe 
                325                 330                 335     


Ile Lys Ala Leu Cys Glu His Asn Leu Asn Gln Asp Asp Leu Asn Glu 
            340                 345                 350         


Ile Ala Lys Asp Ile Thr Leu Val Lys Asp Glu Thr Lys Leu Lys Lys 
        355                 360                 365             


Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Glu Asn Leu Ser 
    370                 375                 380                 


Lys Ile Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala Leu Lys 
385                 390                 395                 400 


Leu Ile Val Pro Leu Met Arg Glu Gly Lys Lys Tyr Asp Glu Val Cys 
                405                 410                 415     


Asn Glu Leu Asn Leu Lys Thr Ala Ile Asn Glu Asp Lys Lys Glu Phe 
            420                 425                 430         


Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Asn Asn Pro 
        435                 440                 445             


Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn Ser Leu 
    450                 455                 460                 


Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Val Gly Lys Asn His Arg Glu Arg Ser Lys Ile Glu Lys Glu Gln 
                485                 490                 495     


Asn Glu Asn Phe Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys Glu Lys 
            500                 505                 510         


Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg Leu Phe 
        515                 520                 525             


Lys Glu Gln Lys Glu Phe Cys Val Tyr Ser Gly Glu Lys Ile Lys Ile 
    530                 535                 540                 


Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile Tyr Pro 
545                 550                 555                 560 


Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu Val Phe 
                565                 570                 575     


Thr Lys Gln Asn Gln Glu Lys Leu Asn Lys Thr Pro Phe Glu Ala Phe 
            580                 585                 590         


Gly Lys Asp Asp Val Lys Trp Gln Lys Ile Glu Thr Leu Ala Lys Asn 
        595                 600                 605             


Leu Pro Ala Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr Lys Asp 
    610                 615                 620                 


Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr Arg Tyr 
625                 630                 635                 640 


Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Glu Phe Leu 
                645                 650                 655     


Pro Leu Ser Asp Asp Glu Asn Ile Lys Leu Asn Asp Thr Gln Lys Gly 
            660                 665                 670         


Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser Val Leu 
        675                 680                 685             


Arg His Thr Trp Gly Phe Ser Thr Lys Asp Arg Asn Asn His Leu His 
    690                 695                 700                 


His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Ala Ser Val Val 
705                 710                 715                 720 


Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Asn Tyr Thr Ala Glu 
                725                 730                 735     


Leu Tyr Ala Lys Lys Ile Asn Glu Leu Asp Phe Lys Asn Lys Arg Lys 
            740                 745                 750         


Phe Phe Glu Pro Phe Ser Asn Phe Arg Gln Lys Val Leu Glu Lys Ile 
        755                 760                 765             


Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser Gly Ala 
    770                 775                 780                 


Leu His Glu Glu Thr Phe Tyr Lys Glu Glu Glu Phe Tyr Lys Glu Tyr 
785                 790                 795                 800 


Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys Ile Arg 
                805                 810                 815     


Lys Val Asn Gly Lys Ile Val Ser Asn Ala Asn Met Phe Arg Val Asp 
            820                 825                 830         


Ile Phe Lys Tyr Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro Ile Tyr 
        835                 840                 845             


Thr Met Asp Phe Ala Leu Gly Ile Leu Pro Asn Lys Ala Val Val Gly 
    850                 855                 860                 


Gly Lys Asp Lys Gln Gly Ile Ile Lys Asp Trp Leu Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Leu 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Leu Val Tyr Phe Lys Ser Phe 
            900                 905                 910         


Lys Ser Ser Thr Ala Gly Leu Val Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Asn Leu Asn Glu Asn Gln Lys Lys Leu Phe Lys Ser Ala Asn Glu 
    930                 935                 940                 


Lys Lys Val Val Ala Glu Gly Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Ile Thr Lys Ala Glu Ile 
                965                 970                 975     


Lys Glu Arg Glu Asn Phe Lys Lys Lys 
            980                 985 


<210>  455
<211>  986
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  455

Met Pro Ser Asp Pro Ala Glu Ala Lys Pro Leu Glu Leu Leu Asp Pro 
1               5                   10                  15      


Tyr Ala Leu Arg Ala Gln Gly Leu Asp Gln Ala Leu Pro Leu Thr His 
            20                  25                  30          


Phe Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe Lys Ser 
        35                  40                  45              


Asn Arg Lys Thr Asp Arg Gly Asp Asn Glu Asn Gly Lys Ile Lys Asp 
    50                  55                  60                  


Ala Ser Gln Arg Leu Asp Trp Ala Met His Glu Ala Lys Ala Arg Thr 
65                  70                  75                  80  


Tyr Gly Glu Phe Leu His Leu Arg Arg Gln Lys Ala Pro Asp Pro Arg 
                85                  90                  95      


Gln Val Pro Thr Val Arg Thr Arg Leu Ser Val Gly Lys Arg Gly Gly 
            100                 105                 110         


Pro Asp Ala Lys Glu Glu Ala Gly Tyr Asp Phe Tyr Pro Asp Arg Arg 
        115                 120                 125             


His Leu Glu Glu Glu Phe His Glu Leu Trp Ser Ala Gln Ala Gln Phe 
    130                 135                 140                 


His Pro Glu Leu Thr Gln Asp Leu Arg Asp Leu Val Phe Glu Lys Ile 
145                 150                 155                 160 


Phe Tyr Gln Arg Pro Leu Lys Glu Pro Lys Val Gly Leu Cys Leu Phe 
                165                 170                 175     


Thr Ser Glu Glu Arg Leu Ala Lys Ala His Pro Leu Thr Gln Arg Arg 
            180                 185                 190         


Val Leu Tyr Glu Thr Val Asn Gln Leu Arg Ile Thr Ala Asp Gly Arg 
        195                 200                 205             


Ser Val Arg Pro Leu Ser Arg Glu Gln Arg Asp Gln Ile Ile Leu Ala 
    210                 215                 220                 


Leu Asp Thr Lys Lys Pro Thr Lys Ser Leu Asn Ser Met Val Val Thr 
225                 230                 235                 240 


Leu Gln Ala Leu Gly Lys Val Leu Lys Leu Arg Glu Gly Glu Arg Phe 
                245                 250                 255     


Thr Leu Glu Ser Gly Val Arg Glu Ala Ile Ala Cys Asp Pro Val Arg 
            260                 265                 270         


Ala Ala Met Leu Ala Thr Phe Gly Pro Arg Trp Ser Ala Leu Asp Trp 
        275                 280                 285             


Pro Glu Gln Trp Gln Val Ile Ser Arg Val Arg Lys Leu Gln Asp Asp 
    290                 295                 300                 


Lys Asp Phe Ala Glu Leu Val Glu Trp Leu Ile Glu Leu Tyr Gly Val 
305                 310                 315                 320 


Asp Lys Glu Ala Ala Val Lys Ile Ala Asn Thr His Leu Pro Glu Gly 
                325                 330                 335     


Tyr Gly Arg Leu Gly Leu Thr Ala Thr Thr Arg Ile Leu Asp Arg Leu 
            340                 345                 350         


Lys Ser Asn Val Phe Thr Tyr Ala Gln Ala Val Ala Ala Cys Gly Trp 
        355                 360                 365             


His His Ser Asp Gln Arg Thr Gly Glu Cys Leu Asp Arg Leu Pro Tyr 
    370                 375                 380                 


Tyr Gly Ala Val Leu Asp Arg His Val Ile Pro Gly Thr Tyr Asp Glu 
385                 390                 395                 400 


Asn Asp Asp Asp Ile Thr Arg Phe Gly Arg Ile Thr Asn Pro Thr Val 
                405                 410                 415     


His Ile Gly Leu Asn Gln Leu Arg Arg Leu Val Asn Lys Ile Ile Glu 
            420                 425                 430         


Val Tyr Gly Lys Pro Asn Gln Ile Val Val Glu Leu Ala Arg Glu Leu 
        435                 440                 445             


Lys Gln Ser Glu Gln Gln Lys Asn Asp Ala Ile Lys Leu Ile Arg Asp 
    450                 455                 460                 


Thr Thr Thr Ala Ala Lys Gln Arg Ser Glu Lys Leu Glu Glu Leu Gly 
465                 470                 475                 480 


Ile Thr Asp Asn Gly Arg Asn Arg Met Leu Leu Arg Leu Trp Glu Asp 
                485                 490                 495     


Leu Asn Pro Asp Asp Ala Met Arg Arg Phe Cys Pro Tyr Thr Gly Gln 
            500                 505                 510         


Arg Ile Ser Ala Ala Met Ile Phe Asp Gly Ser Cys Asp Val Asp His 
        515                 520                 525             


Ile Leu Pro Tyr Ser Arg Thr Leu Asp Asp Gly Phe Ala Asn Arg Thr 
    530                 535                 540                 


Leu Cys Leu Arg Glu Ala Asn Arg Glu Lys Arg Asn Gln Thr Pro Trp 
545                 550                 555                 560 


Glu Ala Trp Gly Gly Thr Pro Asn Trp Glu Ala Ile Glu Thr Asn Leu 
                565                 570                 575     


Lys Asn Leu Pro Glu Asn Lys Arg Trp Arg Phe Ala Pro Asp Ala Met 
            580                 585                 590         


Ala Arg Phe Glu Gly Glu Asn Asp Phe Leu Asp Arg Ala Leu Val Asp 
        595                 600                 605             


Thr Gln Tyr Leu Ala Arg Ile Ser Arg Thr Tyr Leu Asp Thr Leu Phe 
    610                 615                 620                 


Thr Glu Gly Gly His Val Trp Val Val Pro Gly Arg Leu Thr Glu Met 
625                 630                 635                 640 


Leu Arg Arg His Trp Gly Leu Asn Ser Leu Leu Thr Asp Lys Asp Arg 
                645                 650                 655     


Gly Ala Val Lys Ser Lys Asn Arg Thr Asp His Arg His His Ser Ile 
            660                 665                 670         


Asp Ala Ala Val Val Ala Ala Thr Asp Arg Ser Leu Leu Asn Arg Ile 
        675                 680                 685             


Ser Arg Ala Ala Gly Gln Gly Glu Val Ala Gly Gln Ser Ala Glu Leu 
    690                 695                 700                 


Ile Ala Arg Glu Thr Pro Pro Pro Trp Glu Gly Phe Arg Asn Asp Leu 
705                 710                 715                 720 


Lys Ser Gln Leu Gly Arg Ile Ile Val Ser His Arg Ala Asp His Gly 
                725                 730                 735     


Arg Ile Asp Arg Gln Ala Arg Lys Gln Gly Arg Asp Ser Thr Ala Gly 
            740                 745                 750         


Gln Leu His Gln Glu Thr Ala Tyr Ser Ile Val Asp Asp Thr Arg Val 
        755                 760                 765             


Ala Ser Arg Ile Asp Phe Leu Ser Val Lys Pro Ala Gln Leu Leu Asp 
    770                 775                 780                 


Gln Pro Gly Arg Ser Gly Gln Val Arg Asp Leu Gln Leu Arg Lys Ala 
785                 790                 795                 800 


Leu Arg Val Ala Thr Gly Asp Leu Thr Gly Lys Asp Tyr Glu Lys Ala 
                805                 810                 815     


Leu Arg Asp Phe Ala Ala Lys Pro Gly Pro Tyr Lys Gly Ile Arg Arg 
            820                 825                 830         


Val Arg Ile Ile Lys Pro Leu Gln Glu Gln Ala Arg Val Pro Val Pro 
        835                 840                 845             


Val Leu Asp Pro Ile Lys Ala Tyr Gln Gly Gly Ser Asn His Val Phe 
    850                 855                 860                 


Glu Ile Trp His Leu Pro Asp Gly Lys Val Glu Pro Gln Val Ile Thr 
865                 870                 875                 880 


Ser Phe Glu Ala His Ala Leu Glu Val Glu Lys Arg Pro His Pro Ala 
                885                 890                 895     


Ala Lys Arg Leu Leu Arg Val His Lys Gly Asp Met Val Ala Leu Lys 
            900                 905                 910         


Arg Glu Gly Arg Thr Val Val Gly His Val Gln Gln Met His Ile Lys 
        915                 920                 925             


Asn Gly Leu Phe Ile Val Pro His Asn Glu Ala Asn Ala Asp Ala Arg 
    930                 935                 940                 


Asn Ser Asp Arg Thr Asp Pro Phe Lys Trp Ile Gln Ile Ala Ala Gln 
945                 950                 955                 960 


Pro Ala Ile Ala Ala Gly Ile Arg Arg Val Ala Val Asp Glu Ile Gly 
                965                 970                 975     


Arg Leu Arg Asp Gly Gly Ala Lys Thr Ile 
            980                 985     


<210>  456
<211>  987
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  456

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ser Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Phe 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Asn Gly Asp Glu Glu Lys Gly Glu 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Val Ser Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Ile Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Gln Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Thr Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asn Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Gln Lys Tyr Lys 
                325                 330                 335     


Glu Phe Val Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Val Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Lys Thr Pro Phe Glu 
            580                 585                 590         


Thr Phe Gly Asn Asp Arg Val Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Thr Leu Arg His Thr Trp Gly Phe Ser Thr Lys Asp Arg Asp Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Ala Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Thr 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Leu Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Met Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Val Gln Gly Lys Asp Lys Lys Ser Gly Leu Ile Lys Asp Trp Ile Leu 
865                 870                 875                 880 


Met Asp Glu Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu 
                885                 890                 895     


Ile Leu Ile Gln Thr Lys Asp Met Gln Glu Pro Glu Leu Val Tyr Tyr 
            900                 905                 910         


Glu Arg Leu Asp Ser Ser Asn Ala Ser Leu Thr Val Ser Lys His Asp 
        915                 920                 925             


Asn Lys Phe Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn 
    930                 935                 940                 


Ala Asn Glu Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu 
945                 950                 955                 960 


Lys Val Phe Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys 
                965                 970                 975     


Ala Glu Phe Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 985         


<210>  457
<211>  987
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  457

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Asp His Ser Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn Tyr Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Leu Val Tyr Phe Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Asn Glu Leu Arg 
            980                 985         


<210>  458
<211>  987
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  458

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Asn Gly Asp Glu Glu Lys Ser Glu 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Val Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Ile Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Gln Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Val Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Asp Glu His Ser Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Lys Glu Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Ile Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Val Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Leu Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Ala Lys Gln Asn Gln Glu Lys Leu Asn Lys Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Thr Lys Trp Gln Lys Ile Glu Val Leu Thr 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Thr Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Leu Val Tyr Phe Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Asn Glu Leu Arg 
            980                 985         


<210>  459
<211>  987
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  459

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Thr Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Val Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asn Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Leu Val Tyr Phe Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Asn Glu Leu Arg 
            980                 985         


<210>  460
<211>  987
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  460

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Ser Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Asp His Ser Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn Tyr Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Lys Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Leu Val Tyr Phe Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Asn Glu Leu Arg 
            980                 985         


<210>  461
<211>  987
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  461

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Asp His Ser Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Ile Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn Tyr Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asp Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Asn Glu Leu Arg 
            980                 985         


<210>  462
<211>  992
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  462

Met Arg Ile Phe Ser Asp Gly Arg Asn Pro Lys Asp Gly Ser Ser Leu 
1               5                   10                  15      


Ala Val Thr Arg Arg Ala Ala Arg Ala Met Arg Arg Arg Arg Asp Arg 
            20                  25                  30          


Leu Leu Lys Arg Lys Thr Arg Met Gln Ala Lys Leu Val Glu His Gly 
        35                  40                  45              


Phe Phe Pro Ala Asp Ala Gly Lys Arg Lys Ala Leu Glu Gln Leu Asn 
    50                  55                  60                  


Pro Tyr Ala Leu Arg Ala Lys Gly Leu Gln Glu Ala Leu Leu Pro Gly 
65                  70                  75                  80  


Glu Phe Ala Arg Ala Leu Phe His Ile Asn Gln Arg Arg Gly Phe Lys 
                85                  90                  95      


Ser Asn Arg Lys Thr Asp Lys Lys Asp Asn Asp Ser Gly Val Leu Lys 
            100                 105                 110         


Lys Ala Ile Gly Gln Leu Arg Gln Gln Met Ala Glu Gln Gly Ser Arg 
        115                 120                 125             


Thr Val Gly Glu Tyr Leu Trp Thr Arg Leu Gln Gln Gly Gln Gly Val 
    130                 135                 140                 


Arg Ala Arg Tyr Arg Glu Lys Pro Tyr Thr Thr Glu Glu Gly Lys Lys 
145                 150                 155                 160 


Arg Ile Asp Lys Ser Tyr Asp Leu Tyr Ile Asp Arg Ala Met Ile Glu 
                165                 170                 175     


Gln Glu Phe Asp Ala Leu Trp Ala Ala Gln Ala Ala Phe Asn Pro Thr 
            180                 185                 190         


Leu Phe His Glu Ala Ala Arg Ala Asp Leu Lys Asp Thr Leu Leu His 
        195                 200                 205             


Gln Arg Pro Leu Arg Pro Val Lys Pro Gly Arg Cys Thr Leu Leu Pro 
    210                 215                 220                 


Glu Glu Glu Arg Ala Pro Leu Ala Leu Pro Ser Thr Gln Arg Phe Arg 
225                 230                 235                 240 


Ile His Gln Glu Val Asn His Leu Arg Leu Leu Asp Glu Asn Leu Arg 
                245                 250                 255     


Glu Val Ala Leu Thr Leu Ala Gln Arg Asp Ala Val Val Thr Ala Leu 
            260                 265                 270         


Glu Thr Lys Ala Lys Leu Ser Phe Glu Gln Ile Arg Lys Leu Leu Lys 
        275                 280                 285             


Leu Ser Gly Ser Val Gln Phe Asn Leu Glu Asp Ala Lys Arg Thr Glu 
    290                 295                 300                 


Leu Lys Gly Asn Ala Thr Ser Ala Ala Leu Ala Arg Lys Glu Leu Phe 
305                 310                 315                 320 


Gly Ala Ala Trp Ser Gly Phe Asp Glu Ala Leu Gln Asp Glu Ile Val 
                325                 330                 335     


Trp Gln Leu Val Thr Glu Glu Gly Glu Gly Ala Leu Ile Ala Trp Leu 
            340                 345                 350         


Gln Thr His Thr Gly Val Asp Glu Ala Arg Ala Gln Ala Ile Val Asp 
        355                 360                 365             


Val Ser Leu Pro Glu Gly Tyr Gly Asn Leu Ser Arg Lys Ala Leu Ala 
    370                 375                 380                 


Arg Ile Val Pro Ala Leu Arg Ala Ala Val Ile Thr Tyr Asp Lys Ala 
385                 390                 395                 400 


Val Gln Ala Ala Gly Phe Asp His His Ser Gln Leu Gly Phe Glu Tyr 
                405                 410                 415     


Asp Ala Ser Glu Val Glu Asp Leu Val His Pro Glu Thr Gly Glu Ile 
            420                 425                 430         


Arg Ser Val Phe Lys Gln Leu Pro Tyr Tyr Gly Lys Ala Leu Gln Arg 
        435                 440                 445             


His Val Ala Phe Gly Ser Gly Lys Pro Glu Asp Pro Asp Glu Lys Arg 
    450                 455                 460                 


Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ile Gly Leu Asn Gln Val 
465                 470                 475                 480 


Arg Met Val Val Asn Ala Leu Ile Arg Arg Tyr Gly Arg Pro Thr Glu 
                485                 490                 495     


Val Val Ile Glu Leu Ala Arg Asp Leu Lys Gln Ser Arg Glu Gln Lys 
            500                 505                 510         


Val Glu Ala Gln Arg Arg Gln Ala Asp Asn Gln Arg Arg Asn Ala Arg 
        515                 520                 525             


Ile Arg Arg Ser Ile Ala Glu Val Leu Gly Ile Gly Glu Glu Arg Val 
    530                 535                 540                 


Arg Gly Ser Asp Ile Gln Lys Trp Ile Cys Trp Glu Glu Leu Ser Phe 
545                 550                 555                 560 


Asp Ala Ala Asp Arg Arg Cys Pro Tyr Ser Gly Val Gln Ile Ser Ala 
                565                 570                 575     


Ala Met Leu Leu Ser Asp Glu Val Glu Val Glu His Ile Leu Pro Phe 
            580                 585                 590         


Ser Lys Thr Leu Asp Asp Ser Leu Asn Asn Arg Thr Val Ala Met Arg 
        595                 600                 605             


Gln Ala Asn Arg Ile Lys Arg Asn Arg Thr Pro Trp Asp Ala Arg Ala 
    610                 615                 620                 


Glu Phe Glu Ala Gln Gly Trp Ser Tyr Glu Asp Ile Leu Gln Arg Ala 
625                 630                 635                 640 


Glu Arg Met Pro Leu Arg Lys Arg Tyr Arg Phe Ala Pro Asp Gly Tyr 
                645                 650                 655     


Glu Arg Trp Leu Gly Asp Asp Lys Asp Phe Leu Ala Arg Ala Leu Asn 
            660                 665                 670         


Asp Thr Arg Tyr Leu Ser Arg Val Ala Ala Glu Tyr Leu Arg Leu Val 
        675                 680                 685             


Cys Pro Gly Thr Arg Val Ile Pro Gly Gln Leu Thr Ala Leu Leu Arg 
    690                 695                 700                 


Gly Lys Phe Gly Leu Asn Asp Val Leu Gly Leu Asp Gly Glu Lys Asn 
705                 710                 715                 720 


Arg Asn Asp His Arg His His Ala Val Asp Ala Cys Val Ile Gly Val 
                725                 730                 735     


Thr Asp Gln Gly Leu Met Gln Arg Phe Ala Thr Ala Ser Ala Gln Ala 
            740                 745                 750         


Arg Gly Asp Gly Leu Thr Arg Leu Val Asp Gly Met Pro Met Pro Trp 
        755                 760                 765             


Pro Thr Tyr Arg Asp His Val Glu Arg Ala Val Arg His Ile Trp Val 
    770                 775                 780                 


Ser His Arg Pro Asp His Gly Phe Glu Gly Ala Met Met Glu Glu Thr 
785                 790                 795                 800 


Ser Tyr Gly Ile Arg Lys Asp Gly Ser Ile Lys Gln Arg Arg Lys Ala 
                805                 810                 815     


Asp Gly Ser Ala Gly Arg Glu Ile Ser Asn Leu Ile Arg Ile His Glu 
            820                 825                 830         


Ala Thr Gln Pro Leu Arg His Gly Val Ser Ala Asp Gly Gln Pro Leu 
        835                 840                 845             


Ala Tyr Lys Gly Tyr Val Gly Gly Ser Asn Tyr Cys Ile Glu Ile Thr 
    850                 855                 860                 


Val Asn Asp Lys Gly Lys Trp Glu Gly Glu Val Ile Ser Thr Phe Arg 
865                 870                 875                 880 


Ala Tyr Gly Val Val Arg Ala Gly Gly Met Gly Arg Leu Arg Asn Pro 
                885                 890                 895     


His Glu Gly Gln Asn Gly Arg Lys Leu Ile Met Arg Leu Val Ile Gly 
            900                 905                 910         


Asp Ser Val Arg Leu Glu Val Asp Gly Ala Glu Arg Thr Met Arg Ile 
        915                 920                 925             


Val Lys Ile Ser Gly Ser Asn Gly Gln Ile Phe Met Ala Pro Ile His 
    930                 935                 940                 


Glu Ala Asn Val Asp Ala Arg Asn Thr Asp Lys Gln Asp Ala Phe Thr 
945                 950                 955                 960 


Tyr Thr Ser Lys Tyr Ala Gly Ser Leu Gln Lys Ala Lys Thr Arg Arg 
                965                 970                 975     


Val Thr Ile Ser Pro Ile Gly Glu Val Arg Asp Pro Gly Phe Lys Gly 
            980                 985                 990         


<210>  463
<211>  994
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  463

Met Thr Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp Ala Leu 
1               5                   10                  15      


Ile Asp Phe Asp Asp Asn Gln Gln Pro Arg Asn Leu Ile Ala Cys Gly 
            20                  25                  30          


Ser Arg Ile Phe Gln Glu Ala Val Glu Ala Lys Thr Gly Thr Pro Lys 
        35                  40                  45              


Asn His Ala Arg Arg Thr Ala Arg Ala Ala Arg Lys Leu Val Ala Arg 
    50                  55                  60                  


Arg Lys Gln Arg Arg Thr Lys Leu Leu Asn Leu Leu Ile Arg His Gly 
65                  70                  75                  80  


Leu Leu Pro Gln Asp Val Ala Glu Arg Asn Ala Leu Leu Leu Asp Asn 
                85                  90                  95      


Gln Ala Asn Asn Pro Tyr Gln Leu Arg Lys Arg Gly Leu Asp Glu Lys 
            100                 105                 110         


Leu Glu Pro Phe Ala Phe Gly Arg Ala Leu Tyr His Leu Ala His Arg 
        115                 120                 125             


Arg Gly Phe Gln Ser Asn Arg Lys Ala Ala Ser Asp Glu Asp Gly Lys 
    130                 135                 140                 


Val Lys Thr Ala Ile Ser Thr Leu Arg Ala Glu Met Gln Gly Arg Thr 
145                 150                 155                 160 


Leu Gly Ala Phe Leu Ala Asp Gln Pro Thr Gln Arg Arg Arg Tyr Thr 
                165                 170                 175     


Asp Arg Ala Met Tyr Val Glu Glu Phe Glu Gln Leu Trp Ser Lys Gln 
            180                 185                 190         


Ala Ser Phe Thr Gln Glu Gln Leu Thr Gln Ala Leu Lys Val Ala Ile 
        195                 200                 205             


His Asn Ser Ile Phe Phe Gln Arg Pro Leu Lys Ser Gln Lys His Leu 
    210                 215                 220                 


Val Gly Lys Cys Thr Phe Glu Pro Thr Arg Lys Arg Ala Ala Lys Ala 
225                 230                 235                 240 


Ile Leu Leu Thr Gln Arg Phe Arg Ile Leu Gln Asp Val Asn His Leu 
                245                 250                 255     


Ala Val Lys Asn Pro Ile Thr Arg Glu Phe Arg Pro Leu Thr Gln Asp 
            260                 265                 270         


Glu Arg Asn Lys Leu Ala Glu Ala Leu Asp Lys Gln Lys Ser Met Thr 
        275                 280                 285             


Trp Gly Ala Val Arg Lys Val Ile Gly Trp Asn Lys Lys Asp Ser Leu 
    290                 295                 300                 


His Glu Gly Glu Thr Phe Asn Leu Glu Glu Gly Lys Lys Asp Lys Leu 
305                 310                 315                 320 


Ile Gly Asn Arg Thr Ala Cys Asp Leu Arg Gly Ala Leu Gly Asp Arg 
                325                 330                 335     


Trp Asp Ala Leu Thr Ala Gln Gln Gln Asp Asp Leu Val Thr Asp Leu 
            340                 345                 350         


Leu Thr Ile Asp Asn Thr Asp Gly Phe Leu Lys Arg Met Lys Thr His 
        355                 360                 365             


Trp Glu Phe Asp Asp Ala Thr Ala Glu Val Ile Ala Lys Ile Glu Leu 
    370                 375                 380                 


Glu Pro Gly Tyr Ala Arg Leu Ser Ala Lys Ala Met Arg Ala Ile Leu 
385                 390                 395                 400 


Pro Tyr Leu Glu Gln Gly Met Ile Tyr Ser Asp Ala Cys Lys Ala Ala 
                405                 410                 415     


Gly Tyr Asp His Ser Asn Pro Asn Gln Arg Thr Ile Thr Asp Lys Leu 
            420                 425                 430         


Gly Leu Pro Ser Asn Val Arg Asn Pro Val Val Gln Lys Ala Leu Phe 
        435                 440                 445             


Glu Thr Arg Lys Val Val Asn Ala Ile Val Arg Lys Tyr Gly Lys Pro 
    450                 455                 460                 


Ala Val Ile Arg Val Glu Met Ala Arg Asp Met Lys Leu Ser Lys Arg 
465                 470                 475                 480 


Gln Lys Glu Glu Leu Gln Lys Glu Gln Asn Lys Gln Lys Lys Ala Asn 
                485                 490                 495     


Glu Leu Ala Glu Gly Ile Leu Arg Lys Glu Phe Gly Ile Gln Gln Pro 
            500                 505                 510         


Ala Arg Ala Asp Ile Gln Lys Tyr Asn Leu Trp Leu Glu Cys Lys Glu 
        515                 520                 525             


Val Cys Pro Tyr Thr Gly Thr Thr Ile Ser Arg Glu Met Leu Phe Ser 
    530                 535                 540                 


Pro Glu Val Asp Val Glu His Ile Leu Pro Tyr Ser Arg Ser Leu Asp 
545                 550                 555                 560 


Asp Ser Tyr Met Asn Lys Thr Leu Cys Met Ala Thr Phe Asn Arg Gln 
                565                 570                 575     


Val Lys Leu Asn Asn Thr Pro Tyr Glu Ile Gly Gln Gly Asp Pro Leu 
            580                 585                 590         


Ala Tyr Ala Glu Ile Phe Gln Arg Ile Lys Thr Leu Pro Phe Pro Lys 
        595                 600                 605             


Arg Arg Arg Phe Glu Gln Lys Glu Ile Glu Thr Asp Lys Phe Ile Glu 
    610                 615                 620                 


Arg Gln Leu Asn Asp Thr Arg Tyr Ile Cys Val Glu Val Arg Asp Phe 
625                 630                 635                 640 


Leu Gln Gln Leu Gly Ala Pro Val Glu Val Ser Lys Gly Glu Ala Thr 
                645                 650                 655     


Ala Lys Leu Arg Gly Arg Trp Lys Leu Asn Thr Leu Leu Ser Pro Asp 
            660                 665                 670         


Gly Ser Asn Glu Lys Tyr Arg Gly Asp His Arg His His Ala Ile Asp 
        675                 680                 685             


Ala Val Val Ile Ala Leu Thr Asn Arg Ser Leu Phe His Lys Leu Ser 
    690                 695                 700                 


Arg Leu Ser Ala Gln Ser Ile Ser Leu Asp Lys Ser Arg Phe Asp Val 
705                 710                 715                 720 


Pro Pro Pro Trp Asp Gly Phe Tyr Asp Asp Val Tyr Glu Lys Ile Asp 
                725                 730                 735     


Lys Val Ile Val Ser His Ala Pro Ala Arg Lys Ile Gly Gly Ala Leu 
            740                 745                 750         


His Glu Asp Thr Ala Tyr Gly His Phe Lys Glu Glu Val Ala Asp Pro 
        755                 760                 765             


Lys Thr Gly Lys Val Ser Ile Glu Asp Arg Phe Val Tyr Arg Lys Pro 
    770                 775                 780                 


Leu Ala Thr Leu Thr Ala Asn Glu Val Gly Lys Ile Arg Asp Ala Lys 
785                 790                 795                 800 


Val Arg Glu Phe Val Glu Ala Arg Leu Ala Gln Phe Gly Asn Asn Ser 
                805                 810                 815     


Lys Thr Ala Phe Gly Asp Pro Asn Asn Pro Leu Leu His Ala Asp Gly 
            820                 825                 830         


Lys Thr Pro Ile Arg Thr Val Arg Leu Val Thr Asn Phe Asn Lys Ala 
        835                 840                 845             


Thr Thr His Ala Ile Gln Lys Gly Gln Gly Arg Asp Tyr Lys Phe Phe 
    850                 855                 860                 


Lys Tyr Gly Asn Asn His His Val Glu Ile Ile Glu His Val Glu Thr 
865                 870                 875                 880 


Gly Lys Arg Lys Gly Ile Phe Val Thr Ala Met Glu Ala Ala Arg Arg 
                885                 890                 895     


Val Arg Gly Val Ala Asn Thr Glu Lys Ser Asp Ile Val Gln Arg Asn 
            900                 905                 910         


His Gly Glu Glu Trp Arg Phe Val Met Ser Leu Ser Ile Asn Asp Leu 
        915                 920                 925             


Val Ser Ile Gln Glu Ala Asp Gln Val Arg Ile Met Arg Val Gln Leu 
    930                 935                 940                 


Phe Asp Ala Ser Asn Gly Asn Ile Ile Phe Arg Asp Gln Lys Ser Ala 
945                 950                 955                 960 


Met Leu Asp Asp Lys Leu Ser Arg Tyr Gln Thr Lys Ala His Leu Leu 
                965                 970                 975     


Val Cys Gln Lys Ile Thr Thr Asp Pro Leu Gly Asn Leu Ala Pro Cys 
            980                 985                 990         


Asn Asp 
        


<210>  464
<211>  995
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  464

Met Ser Lys Val Leu Gly Leu Asp Ile Gly Ala Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Asp Asp Glu Glu Asn Asp Ile Ile Gly Ala Gly Val Arg 
            20                  25                  30          


Val Phe Lys Glu Gly Val Asn Leu Lys Gly Val Lys Glu Glu Ser Lys 
        35                  40                  45              


Asn Val Glu Arg Arg Ala Ala Arg Leu Ala Arg Arg Gln His Lys Arg 
    50                  55                  60                  


Arg Ser Gln Arg Arg Asn Arg Leu Val Ala Leu Leu Lys Thr Leu Glu 
65                  70                  75                  80  


Met Phe Pro Glu Asn Asn Glu Asp Ile Ser Asp Tyr Tyr Ala Gln Asp 
                85                  90                  95      


Pro Tyr Glu Leu Arg Lys Lys Gly Leu Tyr Glu Lys Leu Thr Met Tyr 
            100                 105                 110         


Glu Phe Gly Arg Val Leu Leu His Ile Cys Arg Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Ser Arg Lys Thr Asp Ile Ser Glu Glu Gly Lys Ile Phe Glu Gly 
    130                 135                 140                 


Lys Asp Gly Ile Val Gly Ile Asn Gln Thr Arg Asp Ser Ile Arg Glu 
145                 150                 155                 160 


Gly Gly Phe Lys Thr Ile Gly Glu Tyr Leu Ala Ser Leu Asp Pro His 
                165                 170                 175     


Val Ile Arg Arg Arg Asn Arg Phe Thr Leu Arg Asp Met Tyr Glu Glu 
            180                 185                 190         


Glu Phe Asn Leu Met Trp Glu Lys Gln Gln Ser Phe Tyr Pro Leu Gln 
        195                 200                 205             


Leu Ser Asn Glu Phe Lys Glu Ala Ile Ala Glu Val Ile Leu Ser Gln 
    210                 215                 220                 


Arg Lys Leu Lys Ser Gln Lys Ala Leu Val Gly Asn Cys Gly Leu Glu 
225                 230                 235                 240 


Pro Asn Lys Lys Thr Ala Pro Lys Ser Ser Pro Ala Phe Gln Tyr Phe 
                245                 250                 255     


Arg Ile Leu Glu Gln Leu Ser Arg Ile Leu Ile Ser Ile Pro His Thr 
            260                 265                 270         


Gly Arg Tyr Asn Asp Pro Leu Asn Glu Glu Glu Arg Arg Met Leu Ile 
        275                 280                 285             


Asp Lys Leu Ser Val Thr Glu Lys Leu Lys Phe Ser Asp Met Arg Lys 
    290                 295                 300                 


Met Leu Asn Leu Pro Gly Glu Ser Asp Ile Asn Ile Glu Glu Glu Gly 
305                 310                 315                 320 


Gly Arg Leu Leu Gly Asn Thr Thr Tyr Ala Arg Leu Ile Lys Val Phe 
                325                 330                 335     


Gly Lys Lys Arg Trp Ala Gly Phe Ser Glu Glu Glu Lys Glu Gln Ile 
            340                 345                 350         


Trp His Thr Phe His Phe Ala Asp Asn His Glu Trp Leu Lys Glu Tyr 
        355                 360                 365             


Ala Leu Lys Lys Trp Ser Ile Thr Pro Asp Glu Ile Lys Glu Leu Gly 
    370                 375                 380                 


Lys Val Ala Leu Glu Lys Glu Tyr Ala Arg Phe Ser Lys Lys Ser Leu 
385                 390                 395                 400 


Lys Lys Ile Ile Pro Gln Leu Glu Glu Gly Leu Thr Leu Asp Lys Ala 
                405                 410                 415     


Lys Ile Glu Ala Gly Tyr Thr Pro Glu Tyr Glu Ile Glu Lys Ser Leu 
            420                 425                 430         


Ser Ala Tyr Leu Pro Leu Pro Glu Asn Ile Arg Asn Pro Ile Val Gln 
        435                 440                 445             


Gln Ala Leu Tyr Glu Leu Arg His Val Val Asn Ala Val Ile Arg Thr 
    450                 455                 460                 


Tyr Gly Lys Pro Asp Ile Ala Arg Val Glu Leu Ala Arg Glu Met Lys 
465                 470                 475                 480 


Lys Pro Lys Gln Lys Arg Glu Lys Met Arg Ser Glu Leu Arg Lys Arg 
                485                 490                 495     


Gln Met Lys Phe Glu Glu Ile Lys Ser Ile Leu Lys Glu Arg Leu Gly 
            500                 505                 510         


Phe Ile Asp Pro Lys Arg Asp Glu Ile His Lys Tyr Met Leu Trp Glu 
        515                 520                 525             


Glu Cys Arg Glu Thr Cys Pro Tyr Thr Gly Arg Lys Ile Ser Leu Ser 
    530                 535                 540                 


Ala Leu Tyr Gly Gly Asp Phe Glu Val Glu His Ile Ile Pro Tyr Lys 
545                 550                 555                 560 


Arg Ser Leu Asp Asp Ser Leu Phe Asn Lys Thr Val Cys Leu Arg Glu 
                565                 570                 575     


Glu Asn Gln Ile Lys Gly Asp Arg Thr Pro Tyr Glu Ala Tyr Ser Gly 
            580                 585                 590         


Asp Arg Gln Lys Tyr Glu Glu Ile Ile Leu Arg Val Gln Lys Thr Ser 
        595                 600                 605             


Met Pro Arg Gly Lys Lys Asn Leu Phe Ala Ile Lys Glu Leu Lys Asp 
    610                 615                 620                 


Asp Phe Ala His Arg Gln Leu Ser Asp Thr Ala Tyr Ile Ser Arg Glu 
625                 630                 635                 640 


Thr Arg Lys Tyr Leu Gly Ser Thr Ile Lys Lys Val Gln Ala Val Lys 
                645                 650                 655     


Gly Gly Ser Thr Ala Lys Leu Arg Tyr Tyr Trp Gly Leu Asn Ser Ile 
            660                 665                 670         


Leu Gly Val Arg Asp Ile Lys Met Arg Asp Asp His Arg His His Ala 
        675                 680                 685             


Val Asp Ala Leu Val Ile Ala Asn Thr Thr Tyr Ala Phe Val Asn Thr 
    690                 695                 700                 


Leu Ser Arg Tyr His Ser Met Asp Val Glu Pro His Glu Ser Arg Phe 
705                 710                 715                 720 


Pro Leu Pro Trp Glu Gln Phe Arg Asn Asp Ala Glu Ser Val Val Arg 
                725                 730                 735     


Asn Ile Phe Val Ser His Arg Leu Gly Lys Lys Ala Thr Gly Gln Leu 
            740                 745                 750         


His Glu Glu Thr Tyr Tyr Gly Lys Ile Arg Asn Thr Asp Gly Glu Pro 
        755                 760                 765             


Tyr Phe Val Val Arg Lys Lys Leu Gly Thr Leu Thr Ala Lys Gln Ile 
    770                 775                 780                 


Ala Asn Ile Val Asp Pro Val Val Lys Thr Gln Val Phe Glu Arg Leu 
785                 790                 795                 800 


Glu Glu Lys Gly Ile Asp Ile Glu Ser Ser Asn Lys Ile Pro Ala Asp 
                805                 810                 815     


Ala Phe Val Glu Thr Leu Tyr Met Pro Asp Arg Lys Thr Pro Ile Lys 
            820                 825                 830         


Ser Val Arg Val Lys Val Pro Ser Thr Thr Met Ile Gln Leu Tyr Lys 
        835                 840                 845             


Asp Lys Glu Leu Tyr Val Asp Pro Gly Ser Asn His His Ile Val Ile 
    850                 855                 860                 


Phe Glu Asn Glu Glu Gly Lys Arg Asp Gly Ile Val Val Ser Leu Phe 
865                 870                 875                 880 


Glu Ala Ala Gln Arg Leu Arg Arg Asn Glu Pro Val Ile Gln Lys Thr 
                885                 890                 895     


Trp Lys Pro Glu Trp His Phe Val Met Ser Leu Ser Ile Asp Glu Leu 
            900                 905                 910         


Ile Leu Leu Asp Val Asp Glu Lys Asn Ile Asp Trp Gly Asp Thr Gly 
        915                 920                 925             


Leu Ala Leu Arg Tyr Ser Lys Ala Leu Tyr Arg Val Gln Lys Met Asp 
    930                 935                 940                 


Val Asn Lys Ile Ile Thr Leu Arg His His Leu Val Ala Arg Leu Lys 
945                 950                 955                 960 


Asp Asp Glu Gly Lys Glu Phe Gly Arg Glu His Arg Ile Pro Ser Thr 
                965                 970                 975     


Leu Arg Gly Leu Lys Val Lys Ile Asp His Ile Gly Arg Leu Arg Arg 
            980                 985                 990         


Ala Tyr Asp 
        995 


<210>  465
<211>  995
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  465

Met Lys Val Ile Gly Phe Asp Ile Gly Ile Asn Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Asn Lys Glu Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Asn Ala Arg Ser Ser Arg Lys Arg Leu Lys Arg Arg Lys Leu Arg Leu 
    50                  55                  60                  


Ile Ala Met Lys His Ile Leu Ala Lys Glu Leu Asn Leu Asn Tyr Lys 
65                  70                  75                  80  


Asp Tyr Ile Ala Asn Asp Gly Glu Leu Pro Lys Ala Tyr Glu Gly Lys 
                85                  90                  95      


Leu Ile Ser Pro Tyr Glu Leu Arg Tyr Lys Ala Leu Asn Gln Lys Leu 
            100                 105                 110         


Glu Ala Lys Asp Leu Val Arg Val Ile Leu His Ile Ala Lys His Arg 
        115                 120                 125             


Gly Tyr Met Asn Lys Asn Asp Lys Lys Ser Asn Asp Ala Glu Lys Gly 
    130                 135                 140                 


Lys Ile Leu Ser Ala Leu Lys Asn Asn Ala Leu Lys Leu Glu Lys Tyr 
145                 150                 155                 160 


Gln Ser Val Gly Glu Tyr Phe Tyr Lys Glu Phe Phe Gln Lys Tyr Lys 
                165                 170                 175     


Glu Asn Thr Lys Asp Phe Ile Lys Ile Arg Asn Ile Lys Asp Asn Tyr 
            180                 185                 190         


Asn Asn Cys Val Leu Ala Ser Asp Leu Glu Lys Glu Leu Lys Leu Ile 
        195                 200                 205             


Leu Glu Lys Gln Lys Glu Phe Gly Tyr Ser Tyr Ser Asn Asp Phe Ile 
    210                 215                 220                 


Glu Glu Val Ile Gln Val Ala Phe Phe Gln Arg Pro Leu Lys Asp Phe 
225                 230                 235                 240 


Ser His Leu Val Gly Thr Cys Thr Phe Phe Glu Glu Glu Lys Arg Ala 
                245                 250                 255     


Cys Lys Asn Ser Tyr Ser Ala Trp Glu Phe Val Ala Leu Thr Lys Ile 
            260                 265                 270         


Val Asn Glu Leu Lys Ser Leu Glu Lys Ile Ser Gly Glu Leu Ile Ser 
        275                 280                 285             


Thr Gln Thr Ile Asn Glu Ile Leu Asn Tyr Ile Leu Asp Lys Gly Ser 
    290                 295                 300                 


Ile Thr Tyr Lys Lys Phe Arg Ser Tyr Ile Asn Leu Tyr Glu Ser Ile 
305                 310                 315                 320 


Lys Phe Lys Ser Leu Lys Tyr Asp Lys Asp Asn Ala Glu Ser Ala Lys 
                325                 330                 335     


Leu Ile Asp Phe Arg Lys Leu Val Glu Phe Lys Lys Ala Leu Gly Glu 
            340                 345                 350         


His Tyr Phe Asn Arg Gln Glu Leu Asp Gln Ile Ser Thr Tyr Ile Thr 
        355                 360                 365             


Leu Ile Lys Asp Asn Val Lys Leu Lys Thr Ala Leu Glu Lys Tyr Asn 
    370                 375                 380                 


Leu Ser Asn Glu Gln Ile Asn Asn Leu Leu Glu Ile Glu Phe Asn Asp 
385                 390                 395                 400 


His Ile Asn Leu Ser Phe Lys Ala Leu Ser Met Ile Leu Pro Leu Met 
                405                 410                 415     


Lys Glu Gly Lys Lys Tyr Asp Glu Ala Cys Glu Ile Ala Asn Leu Lys 
            420                 425                 430         


Pro Lys Thr Thr Asn Gln Lys Gln Asp Phe Leu Pro Ala Phe Cys Asn 
        435                 440                 445             


Ser Ile Phe Val His Glu Leu Ser Asn Pro Ile Val Asn Arg Ala Ile 
    450                 455                 460                 


Ser Glu Tyr Arg Lys Val Leu Asn Ala Leu Leu Lys Lys Tyr Gly Lys 
465                 470                 475                 480 


Val His Lys Ile His Leu Glu Leu Ala Arg Asp Ile Gly Leu Ser Lys 
                485                 490                 495     


Lys Ala Arg Glu Lys Ile Glu Lys Glu Gln Lys Glu Asn Gln Ala Ile 
            500                 505                 510         


Asn Thr Trp Ala Leu Asn Glu Cys Glu Asn Met Gly Ile Lys Ala Asn 
        515                 520                 525             


Ala Lys Asn Ile Leu Lys Leu Lys Leu Trp Lys Glu Gln Lys Glu Ile 
    530                 535                 540                 


Cys Ile Tyr Ser Gly Lys Lys Ile Ser Ile Glu His Leu Lys Asp Glu 
545                 550                 555                 560 


Lys Ala Leu Glu Val Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp 
                565                 570                 575     


Asp Ser Tyr Met Asn Lys Val Leu Val Phe Thr Lys Glu Asn Gln Glu 
            580                 585                 590         


Lys Leu Asn Lys Thr Pro Phe Glu Ala Phe Gly Lys Asn Ile Glu Lys 
        595                 600                 605             


Trp Asn Lys Ile Gln Thr Leu Ala Gln Ile Leu Pro Tyr Lys Lys Lys 
    610                 615                 620                 


Asn Lys Ile Leu Asp Glu Asn Phe Lys Asp Asn Gln Gln Gln Asp Phe 
625                 630                 635                 640 


Ile Ser Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ala Thr Leu Ile Ala 
                645                 650                 655     


Lys Tyr Thr Lys Glu Tyr Leu Asn Phe Leu Pro Leu Asn Glu Asn Glu 
            660                 665                 670         


Asn Ile Asn Leu Lys Ser Gly Glu Lys Gly Ser Lys Ile His Ile Gln 
        675                 680                 685             


Thr Val Asn Gly Met Leu Thr Ser Val Leu Arg His Thr Trp Gly Phe 
    690                 695                 700                 


Asp Lys Lys Asp Arg Asn Asn His Leu His His Ala Leu Asp Ala Ile 
705                 710                 715                 720 


Ile Val Ala Tyr Ser Thr Asn Ser Leu Ile Lys Ala Phe Ser Asp Phe 
                725                 730                 735     


Lys Lys Asn Gln Glu Leu Leu Lys Ala Lys Leu Tyr Ala Lys Glu Leu 
            740                 745                 750         


Thr Asn Asp Asn Tyr Ile His Gln Ala Lys Phe Phe Glu Pro Phe Glu 
        755                 760                 765             


Gly Phe Arg Glu Lys Ile Leu Asn Lys Ile Asp Glu Ile Phe Val Ser 
    770                 775                 780                 


Lys Pro Pro Arg Lys Arg Ala Arg Gly Ala Leu His Lys Glu Thr Phe 
785                 790                 795                 800 


Tyr Ser Glu Lys Lys Met Ile Lys Lys Tyr Asn Ser Lys Glu Gly Leu 
                805                 810                 815     


Asn Ile Ala Leu Asp Cys Gly Lys Ile Arg Lys Ile Gly Thr Lys Tyr 
            820                 825                 830         


Val Glu Asn Asp Thr Met Val Arg Val Asp Ile Phe Lys Lys Gln Asn 
        835                 840                 845             


Lys Phe Tyr Ala Ile Pro Ile Tyr Thr Met Asp Phe Ala Leu Gly Ile 
    850                 855                 860                 


Leu Pro Asn Lys Ile Val Val Ile Gly Lys Asp Lys Asn Asn Asn Pro 
865                 870                 875                 880 


Lys Gln Trp Gln Glu Ile Asp Glu Ser Tyr Glu Phe Cys Phe Ser Leu 
                885                 890                 895     


Tyr Lys Asn Asp Leu Ile Leu Leu Gln Lys Lys Asp Met Gln Glu Ala 
            900                 905                 910         


Glu Phe Ala Tyr Tyr Asn Gly Phe Asp Ile Asp Ser Ser Arg Ile Asn 
        915                 920                 925             


Ile Lys Lys His Asp Asn Lys Phe Glu Asn Leu Thr Ser Asn Gln Lys 
    930                 935                 940                 


Leu Leu Phe Thr Asn Lys Glu Lys Asn Arg Thr Gly Ile Gln Asn Leu 
945                 950                 955                 960 


Lys Ile Phe Glu Lys Tyr Ile Val Thr Pro Leu Gly Asn Lys Ile Lys 
                965                 970                 975     


Ala Asp Phe Gln Pro Arg Gln Asn Ile Ser Leu Lys Ser Ser Lys Lys 
            980                 985                 990         


Asn Gly Leu 
        995 


<210>  466
<211>  995
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  466

Met Lys Ile Leu Gly Phe Asp Ile Gly Ile Asn Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Asn Lys Glu Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Asn Ala Arg Ser Asn Arg Arg Arg Leu Lys Arg Arg Lys Ser Arg Leu 
    50                  55                  60                  


Ile Ala Ile Lys His Ile Leu Ala Lys Glu Leu Asn Leu Asn Tyr Lys 
65                  70                  75                  80  


Asp Tyr Ile Ala Asn Asp Gly Glu Leu Pro Lys Ala Tyr Glu Gly Lys 
                85                  90                  95      


Leu Thr Asn Val Tyr Glu Leu Arg Tyr Lys Ala Leu Asn Gln Lys Leu 
            100                 105                 110         


Glu Ala Lys Asp Leu Ala Arg Val Ile Leu His Ile Ala Lys His Arg 
        115                 120                 125             


Gly Tyr Met Asn Lys Asn Glu Lys Lys Ser Asn Asp Ser Glu Lys Gly 
    130                 135                 140                 


Lys Ile Leu Ser Ala Leu Lys Asn Asn Ala Leu Lys Leu Glu Lys Tyr 
145                 150                 155                 160 


Gln Ser Val Gly Glu Tyr Phe Tyr Lys Glu Phe Phe Gln Lys Tyr Lys 
                165                 170                 175     


Glu Asn Thr Lys Asn Phe Ile Lys Ile Arg Asn Thr Lys Asp Asn Tyr 
            180                 185                 190         


Asn Asn Cys Val Leu Ala Ser Asp Leu Glu Lys Glu Leu Lys Leu Ile 
        195                 200                 205             


Leu Glu Lys Gln Lys Glu Phe Gly Tyr Ser Tyr Asn Asp Asp Phe Ile 
    210                 215                 220                 


Lys Glu Ile Ile Asn Ile Ala Phe Phe Gln Arg Pro Leu Lys Asp Phe 
225                 230                 235                 240 


Ser His Leu Val Gly Ser Cys Thr Phe Phe Glu Glu Glu Lys Arg Ala 
                245                 250                 255     


Cys Lys Asn Ser Tyr Ser Ala Trp Glu Phe Val Ala Leu Ser Lys Ile 
            260                 265                 270         


Ile Asn Glu Leu Lys Arg Leu Glu Lys Ile Ser Gly Glu Leu Ile Ser 
        275                 280                 285             


Ala Gln Thr Ile Asn Glu Ile Leu Asn His Ile Leu Asp Lys Gly Asn 
    290                 295                 300                 


Ile Thr Tyr Lys Lys Phe Arg Ser Cys Ile Asn Leu His Glu Ser Ile 
305                 310                 315                 320 


Glu Phe Lys Ser Leu Asn Tyr Asp Lys Asp Asn Ala Glu Ser Ala Lys 
                325                 330                 335     


Leu Val Asp Phe Lys Lys Leu Ile Glu Phe Lys Lys Ala Leu Gly Glu 
            340                 345                 350         


His Ser Phe Asn Arg Gln Glu Leu Asp Glu Ile Ala Thr His Ile Thr 
        355                 360                 365             


Leu Ile Lys Asp Asn Asp Lys Leu Lys Thr Ala Leu Glu Lys Tyr Asn 
    370                 375                 380                 


Leu Asn Asn Glu Gln Ile Asn Ala Leu Leu Glu Ile Glu Phe Asn Asp 
385                 390                 395                 400 


His Ile Asn Leu Ser Phe Lys Ala Leu Asn Leu Ile Leu Pro Leu Met 
                405                 410                 415     


Arg Glu Gly Lys Arg Tyr Asp Glu Ala Cys Glu Ile Val Asn Leu Lys 
            420                 425                 430         


Ser Lys Thr Ser Asn Gln Lys Gln Asp Leu Leu Pro Ala Phe Cys Asp 
        435                 440                 445             


Ser Ile Phe Ser Gln Glu Leu Ser Asn Pro Val Val Asn Arg Ala Ile 
    450                 455                 460                 


Ser Glu Tyr Arg Lys Val Leu Asn Ala Leu Leu Lys Lys Tyr Gly Lys 
465                 470                 475                 480 


Val His Lys Ile His Leu Glu Leu Ala Arg Asp Val Gly Leu Ser Lys 
                485                 490                 495     


Lys Ala Arg Glu Lys Ile Glu Lys Glu Gln Lys Glu Asn Lys Glu Ile 
            500                 505                 510         


Asn Asp Trp Ala Leu Asp Glu Cys Glu Lys Phe Gly Leu Lys Ala Asn 
        515                 520                 525             


Asn Lys Asn Ile Leu Lys Leu Lys Leu Trp Lys Glu Gln Lys Glu Ile 
    530                 535                 540                 


Cys Ala Tyr Ser Gly Lys Lys Ile Ser Ile Glu His Leu Lys Asp Glu 
545                 550                 555                 560 


Lys Ala Leu Glu Val Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp 
                565                 570                 575     


Asp Ser Tyr Met Asn Lys Val Leu Val Phe Thr Lys Glu Asn Gln Glu 
            580                 585                 590         


Lys Leu Asn Lys Thr Pro Phe Glu Ala Phe Gly Lys Asn Ile Glu Arg 
        595                 600                 605             


Trp Asn Lys Ile Gln Ala Leu Ala Gln Asn Leu Pro Tyr Lys Lys Lys 
    610                 615                 620                 


Asn Lys Ile Leu Asp Glu Asn Phe Lys Asp Lys Gln Gln Gln Asp Phe 
625                 630                 635                 640 


Ile Ser Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ala Thr Leu Ile Ala 
                645                 650                 655     


Lys Tyr Thr Lys Glu Tyr Leu Asn Phe Leu Pro Leu Asn Glu Asn Glu 
            660                 665                 670         


Asn Thr Asn Leu Lys Ser Gly Glu Lys Gly Ser Lys Ile His Val Gln 
        675                 680                 685             


Thr Ile Asn Gly Met Leu Thr Ser Val Leu Arg His Thr Trp Gly Phe 
    690                 695                 700                 


Asp Lys Lys Asp Arg Asn Asn His Leu His His Ala Leu Asp Ala Ile 
705                 710                 715                 720 


Ile Val Ala Tyr Ser Thr Asn Ser Ile Ile Lys Ala Phe Ser Asp Phe 
                725                 730                 735     


Lys Lys Asn Gln Glu Leu Leu Lys Ala Lys Leu Tyr Ala Lys Glu Leu 
            740                 745                 750         


Thr Ser Asp Asn Tyr Lys His Gln Ala Lys Phe Phe Glu Pro Phe Glu 
        755                 760                 765             


Gly Phe Arg Glu Lys Ile Leu Asn Lys Ile Asp Glu Ile Phe Val Ser 
    770                 775                 780                 


Lys Pro Pro Arg Lys Arg Ala Arg Gly Thr Leu His Lys Glu Thr Phe 
785                 790                 795                 800 


Tyr Pro Glu Glu Thr Met Ile Lys Lys Tyr Asn Ser Lys Glu Gly Leu 
                805                 810                 815     


Asn Ile Ala Leu Asn Cys Gly Lys Ile Arg Lys Ile Gly Thr Lys Tyr 
            820                 825                 830         


Val Glu Asn Asp Thr Met Val Arg Leu Asp Ile Phe Lys Lys Gln Asn 
        835                 840                 845             


Lys Phe Tyr Ala Ile Pro Ile Tyr Thr Met Asp Phe Ala Leu Gly Ile 
    850                 855                 860                 


Leu Pro Asn Lys Ile Val Ile Ala Gly Lys Asp Lys Asn Asn Asn Pro 
865                 870                 875                 880 


Lys Gln Trp Gln Glu Ile Asp Glu Ser Tyr Glu Phe Cys Phe Ser Leu 
                885                 890                 895     


Tyr Lys Asn Asp Leu Ile Leu Leu Gln Lys Lys Asp Met Gln Glu Ala 
            900                 905                 910         


Glu Phe Ala Tyr Tyr Asn Ser Phe Asp Ile Asp Ser Ser Arg Ile Asn 
        915                 920                 925             


Ile Lys Lys His Asp Asn Lys Phe Glu Asn Leu Thr Asp Asn Gln Lys 
    930                 935                 940                 


Leu Leu Phe Thr Asn Gln Glu Lys Asn Arg Thr Gly Ile Gln Asn Leu 
945                 950                 955                 960 


Lys Ile Phe Glu Lys Tyr Ile Val Thr Pro Leu Gly Asp Lys Ile Lys 
                965                 970                 975     


Ala Asp Phe Gln Pro Arg Gln Asn Ile Ser Leu Lys Thr Ser Lys Lys 
            980                 985                 990         


Tyr Gly Leu 
        995 


<210>  467
<211>  996
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  467

Met Met Gly Asn Phe Thr Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Trp Ala Leu Ile Asp Phe Asp Asp Asn Gln Gln Pro Gln Asn Leu 
            20                  25                  30          


Ile Ala Cys Gly Ser Arg Ile Phe Gln Glu Ala Val Glu Ala Lys Thr 
        35                  40                  45              


Gly Thr Pro Lys Asn His Ala Arg Arg Ala Ala Arg Ala Ala Arg Lys 
    50                  55                  60                  


Leu Val Ala Arg Arg Lys Gln Arg Arg Thr Lys Leu Leu Asn Leu Leu 
65                  70                  75                  80  


Val Cys His Asp Leu Leu Pro Gln Asp Glu Ala Glu Arg Asn Ala Leu 
                85                  90                  95      


Leu Leu Asp Asn Gln Ala Asn Asn Pro Tyr Gln Leu Arg Lys Arg Gly 
            100                 105                 110         


Leu Asp Glu Lys Leu Glu Pro Phe Ala Phe Gly Arg Ala Leu Tyr His 
        115                 120                 125             


Leu Ala His Arg Arg Gly Phe Gln Ser Asn Arg Lys Ala Ala Ser Asp 
    130                 135                 140                 


Glu Asp Gly Lys Val Lys Thr Ala Ile Ser Ala Leu Arg Ala Glu Met 
145                 150                 155                 160 


Gln Gly His Thr Leu Gly Ala Phe Leu Ala Asp Gln Pro Thr Gln Arg 
                165                 170                 175     


Arg Arg Tyr Thr Asp Arg Ala Met Tyr Ala Glu Glu Phe Glu Gln Leu 
            180                 185                 190         


Trp Ser Lys Gln Thr Ile Phe Ala Pro Glu Gln Leu Thr Gln Ala Leu 
        195                 200                 205             


Lys Val Glu Ile Lys Asn Ser Ile Phe Phe Gln Arg Pro Leu Lys Ser 
    210                 215                 220                 


Gln Lys His Leu Val Gly Lys Cys Thr Phe Glu Pro Thr Arg Lys Arg 
225                 230                 235                 240 


Ala Ala Lys Ala Ile Leu Leu Ala Gln His Phe Arg Ile Leu Gln Asp 
                245                 250                 255     


Val Asn His Leu Ala Val Lys Asn Pro Ile Thr Arg Glu Phe Arg Pro 
            260                 265                 270         


Leu Thr Gln Asp Glu Arg Asn Lys Leu Ala Glu Ala Leu Asp Lys Gln 
        275                 280                 285             


Lys Gly Met Asn Trp Ser Ala Val Arg Lys Val Ile Gly Trp Asn Lys 
    290                 295                 300                 


Lys Asp Ser Leu His Glu Gly Glu Thr Phe Asn Leu Glu Glu Gly Lys 
305                 310                 315                 320 


Lys Asp Lys Leu Ile Gly Asn Arg Thr Ala Cys Asp Leu Arg Gly Ala 
                325                 330                 335     


Leu Gly Asp Arg Trp Asp Ala Phe Thr Ala Pro Gln Gln Asp Asp Leu 
            340                 345                 350         


Val Thr Asp Leu Leu Thr Ile Asp Asn Pro Asp Gly Phe Leu Lys Arg 
        355                 360                 365             


Met Lys Thr His Trp Gly Phe Asp Asp Ala Thr Ala Glu Ala Leu Ala 
    370                 375                 380                 


Thr Thr Glu Leu Glu Pro Gly Tyr Ala Arg Leu Ser Ser Lys Ala Met 
385                 390                 395                 400 


Arg Ala Ile Leu Pro His Leu Glu Gln Gly Met Ile Tyr Ser Asp Ala 
                405                 410                 415     


Cys Lys Ala Ala Gly Tyr Asp His Ser Asn Pro Asn Gln Arg Thr Ile 
            420                 425                 430         


Thr Asp Lys Leu Gly Leu Pro Ser Asn Val Arg Asn Pro Val Val Gln 
        435                 440                 445             


Lys Ala Leu Phe Glu Thr Arg Lys Val Val Asn Ala Ile Val Arg Lys 
    450                 455                 460                 


Tyr Gly Lys Pro Ala Val Ile Arg Val Glu Met Ala Arg Asp Met Lys 
465                 470                 475                 480 


Leu Ser Lys Arg Gln Lys Glu Glu Leu Gln Lys Glu Gln Asn Lys Gln 
                485                 490                 495     


Lys Lys Ala Asn Glu Leu Ala Glu Gly Ile Leu Arg Lys Glu Phe Gly 
            500                 505                 510         


Ile Gln Gln Pro Ala Arg Thr Asp Ile Gln Lys Tyr Asn Leu Trp Leu 
        515                 520                 525             


Glu Cys Lys Glu Val Cys Pro Tyr Thr Gly Thr Val Ile Ser Arg Glu 
    530                 535                 540                 


Met Leu Tyr Ser Pro Glu Val Asp Val Glu His Ile Leu Pro Tyr Ser 
545                 550                 555                 560 


Arg Ser Leu Asp Asp Ser Tyr Met Asn Lys Thr Leu Cys Met Ala Thr 
                565                 570                 575     


Tyr Asn Arg Gln Thr Lys Leu Asn Asn Thr Pro Tyr Glu Ile Gly Gln 
            580                 585                 590         


Gly Asp Pro Val Ala Tyr Ala Glu Ile Phe Gln Arg Ile Lys Thr Leu 
        595                 600                 605             


Pro Phe Pro Lys Arg Arg Arg Phe Glu Gln Lys Glu Ile Glu Thr Asp 
    610                 615                 620                 


Lys Phe Ile Glu Arg Gln Leu Asn Asp Thr Arg Tyr Ile Cys Val Glu 
625                 630                 635                 640 


Val Arg Asp Phe Leu Gln Gln Leu Gly Ala Pro Val Glu Val Ser Lys 
                645                 650                 655     


Gly Glu Ala Thr Ala Lys Leu Arg Gly Arg Trp Lys Leu Asn Thr Leu 
            660                 665                 670         


Leu Ser Pro Gly Gly Gly Asn Glu Lys Asn Arg Ala Asp His Arg His 
        675                 680                 685             


His Ala Ile Asp Ala Val Val Ile Ala Leu Thr Asn Arg Ser Leu Phe 
    690                 695                 700                 


His Lys Leu Ser Arg Leu Ser Ala Gln Ser Ile Ser Phe Asp Lys Ser 
705                 710                 715                 720 


Arg Phe Asp Val Pro Pro Pro Trp Asp Asp Phe Tyr Asp Asp Val Tyr 
                725                 730                 735     


Glu Lys Ile Asp Lys Val Ile Val Ser His Ala Pro Thr Arg Lys Ile 
            740                 745                 750         


Ser Gly Ala Leu His Glu Ala Thr Ala Tyr Gly His Phe Lys Glu Glu 
        755                 760                 765             


Ile Ala Asp Pro Lys Thr Gly Lys Phe Ser Ile Glu Asp Arg Phe Val 
    770                 775                 780                 


Tyr Arg Lys Pro Leu Thr Thr Leu Thr Ala Asn Glu Val Gly Lys Ile 
785                 790                 795                 800 


Arg Asp Ala Lys Val Arg Glu Leu Val Glu Ala Arg Leu Ala Gln Phe 
                805                 810                 815     


Gly Asn Asn Ser Lys Ala Ala Phe Gly Asp Pro Asn Glu Pro Leu Leu 
            820                 825                 830         


His Ala Asp Gly Lys Thr Pro Ile Arg Thr Ile Arg Leu Thr Thr Asn 
        835                 840                 845             


Phe Asn Lys Thr Thr Thr His Ala Ile Gln Lys Gly Gln Asp Arg Asp 
    850                 855                 860                 


Tyr Lys Phe Phe Lys Tyr Gly Asn Asn His His Val Glu Ile Ile Glu 
865                 870                 875                 880 


His Val Glu Thr Gly Lys Arg Lys Gly Ile Phe Val Thr Ala Met Glu 
                885                 890                 895     


Ala Ala Arg Arg Ala Arg Gly Val Ala Asn Thr Glu Lys Ser Asn Ile 
            900                 905                 910         


Val Gln Arg Asn His Gly Asp Gly Trp Lys Phe Val Met Ser Leu Ala 
        915                 920                 925             


Val Asn Asp Met Val Arg Thr Glu Asp Thr Gln Ala Ile Tyr Arg Val 
    930                 935                 940                 


Gln Lys Leu Asp Gly Ser Asn Asn Arg Phe Thr Leu Arg Ser His Asn 
945                 950                 955                 960 


Ala Ala Thr Leu Asp Asp Lys Gln Thr Gly Val Asp Lys Thr Pro Asn 
                965                 970                 975     


Leu Phe Lys Gly Thr Lys Ile Ile Val Asp Pro Leu Gly Asn Leu Thr 
            980                 985                 990         


Gln Cys Asn Asp 
        995     


<210>  468
<211>  997
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  468

Met Lys Val Leu Val Asp Ala Gly Leu Met Pro Ser Asp Pro Ala Ala 
1               5                   10                  15      


Ala Lys Ala Leu Glu Thr Leu Asp Pro Tyr Glu Leu Arg Ala Thr Gly 
            20                  25                  30          


Leu Asp Val Met Leu Pro Leu Thr His Leu Gly Arg Ala Val Phe His 
        35                  40                  45              


Leu Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Arg Gly 
    50                  55                  60                  


Asp Asn Glu Ser Gly Lys Ile Lys Asp Ala Thr Lys Arg Leu Thr Trp 
65                  70                  75                  80  


Ala Met Gln Glu Ala Gly Ala Arg Thr Tyr Gly Glu Phe Leu His Met 
                85                  90                  95      


Arg Arg Ala Arg Ala Ser Asp Pro Arg Gln Val Pro Ser Val Arg Thr 
            100                 105                 110         


Arg Leu Ser Val Ala Ser Arg Gly Gly Pro Asp Ser Lys Glu Glu Ala 
        115                 120                 125             


Gly Tyr Asp Phe Tyr Pro Glu Arg Ala His Leu Glu Asp Glu Phe His 
    130                 135                 140                 


Ala Leu Trp Ser Ala Gln Ala Ala Phe His Pro Asp Leu Thr Cys Glu 
145                 150                 155                 160 


Leu Arg Asp Val Val Phe Glu Lys Ile Phe Tyr Gln Arg Pro Leu Lys 
                165                 170                 175     


Glu Pro Lys Val Gly Leu Cys Leu Phe Thr Ala Glu Glu Arg Leu Pro 
            180                 185                 190         


Lys Ala His Pro Leu Thr Gly Arg Arg Val Leu Tyr Glu Thr Val Asn 
        195                 200                 205             


Gln Leu Arg Val Thr Ala Asp Gly Arg Thr Thr Arg Pro Leu Thr Leu 
    210                 215                 220                 


Glu Glu Arg Asp Met Ile Val Tyr Ala Leu Asp Asn Lys Lys Pro Val 
225                 230                 235                 240 


Lys Ser Leu Ser Ser Met His Leu Lys Leu Val Ala Leu Gly Lys Leu 
                245                 250                 255     


Val Lys Leu Lys Asp Gly Glu Arg Phe Thr Leu Glu Ser Gly Val Arg 
            260                 265                 270         


Asp Ala Ile Ala Cys Asp Pro Ile Arg Ala Thr Met Gly His Pro Asp 
        275                 280                 285             


Gly Phe Gly Pro Ala Trp Ser Arg Leu Asp Trp Gln Ala Gln Trp Ala 
    290                 295                 300                 


Leu Ile Glu Lys Leu Arg Gln Val Gln Ser Asp Asp Asp Phe Ala Ser 
305                 310                 315                 320 


Leu Val Ala Trp Leu Gly Ala Thr His Asn Leu Ser Glu Asp His Ala 
                325                 330                 335     


Lys Arg Val Ala Asn Leu His Leu Pro Glu Gly Tyr Gly Arg Leu Gly 
            340                 345                 350         


Leu Thr Ala Thr Glu Ala Ile Leu Cys Asn Leu Lys Ala Glu Val Cys 
        355                 360                 365             


Thr Tyr Ser Gln Ala Val Glu Arg Trp Gly Lys His His Ser Asn Gln 
    370                 375                 380                 


Arg Thr Gly Glu Cys Leu Glu Ala Leu Pro Tyr Tyr Gly Glu Val Leu 
385                 390                 395                 400 


Asp Arg His Val Ile Pro Gly Thr Tyr Asn Glu Asp Asp Asp Asp Val 
                405                 410                 415     


Thr Arg Tyr Gly Arg Ile Thr Asn Pro Thr Val His Ile Gly Leu Asn 
            420                 425                 430         


Gln Leu Arg Arg Leu Val Asn Arg Ile Ile Ala Thr Tyr Gly Lys Pro 
        435                 440                 445             


Asp Gln Ile Val Val Glu Leu Ala Arg Glu Leu Lys Gln Ser Glu Ala 
    450                 455                 460                 


Gln Lys Ser Glu Ala Leu Lys Lys Ile Arg Asp Thr Thr Ala Asp Ala 
465                 470                 475                 480 


Ile Arg Arg Gly Arg Gln Leu Glu Glu Ile Gly Gln Lys Asn Thr Gly 
                485                 490                 495     


Ala Asn Arg Leu Leu Leu Arg Leu Trp Glu Asp Leu Gly Pro Ala Val 
            500                 505                 510         


Gly Pro Arg Cys Cys Pro Tyr Thr Gly Lys Pro Ile Ser Val Thr Met 
        515                 520                 525             


Leu Phe Asp Gly Ala Cys Asp Val Asp His Ile Leu Pro Tyr Ser Arg 
    530                 535                 540                 


Thr Leu Asp Asp Ser Phe Ala Asn Arg Thr Ile Cys Leu Arg Glu Ala 
545                 550                 555                 560 


Asn Arg Gln Lys Gly Asp Arg Thr Pro Trp Glu Ala Trp Gly Gly Ser 
                565                 570                 575     


Asp Gln Trp Asp Ala Ile Glu Ala Asn Leu Lys Asp Leu Pro Glu Asn 
            580                 585                 590         


Lys Arg Lys Arg Phe Ala Pro Asp Ala Met Glu Arg Phe Glu Gly Glu 
        595                 600                 605             


Arg Asp Phe Leu Asp Arg Ala Leu Val Asp Thr Gln Tyr Leu Ala Arg 
    610                 615                 620                 


Ile Ser Arg Ala Tyr Leu Asp Thr Leu Phe Thr Glu Gly Gly His Val 
625                 630                 635                 640 


Trp Val Val Pro Gly Arg Met Thr Glu Met Leu Arg Arg His Trp Gly 
                645                 650                 655     


Leu Asn Ala Leu Leu Ser Asp Lys Asp Arg Gly Ala Gly Lys Val Lys 
            660                 665                 670         


Asn Arg Thr Asp His Arg His His Ala Ile Asp Ala Ala Val Ile Ala 
        675                 680                 685             


Ala Thr Asp His Ser Leu Val Asn Arg Ile Ser Lys Ala Ala Gly Gln 
    690                 695                 700                 


Gly Glu Ser Ala Gly Gln Ser Ala Glu Leu Ile Ala Arg Asp Thr Pro 
705                 710                 715                 720 


Glu Pro Trp Glu Gly Phe Arg Asn Asp Ile Ser Ala Gln Ile Ala Arg 
                725                 730                 735     


Ile Val Val Ser His Arg Ala Asp His Gly Arg Ile Asp Val Glu Gly 
            740                 745                 750         


Arg Lys His Gly Lys Asp Ser Thr Ala Gly Arg Leu His Asn Asp Thr 
        755                 760                 765             


Ala Tyr Gly Ile Val Asp Asp His Thr Val Val Ser Arg Thr Pro Leu 
    770                 775                 780                 


Leu Ser Leu Lys Pro Gly Asp Ile Glu Val Thr Thr Lys Gly Lys Asn 
785                 790                 795                 800 


Ile Arg Asp Thr Gln Leu Gln Lys Ala Leu Ser Val Ala Thr Thr Gly 
                805                 810                 815     


Lys Asp Gly Lys Gly Phe Glu Glu Ala Leu Arg Asn Phe Ala Glu Lys 
            820                 825                 830         


Glu Gly Pro Tyr Gln Gly Ile Arg Arg Val Arg Leu Ile Glu Thr Leu 
        835                 840                 845             


Gln Ser Ser Ala Arg Val Glu Val Gly Ser Asp Glu Asp Gly Gln Pro 
    850                 855                 860                 


Leu Lys Ala Tyr Lys Gly Asp Ser Asn His Cys Tyr Asp Leu Trp Lys 
865                 870                 875                 880 


Leu Pro Asp Gly Lys Val Val Pro His Val Val Ser Thr Tyr Glu Ala 
                885                 890                 895     


His Ala Gly Thr Asp Lys Arg Pro His Pro Ala Ala Lys Arg Ile Leu 
            900                 905                 910         


Arg Val Phe Lys Lys Asp Met Val Ala Ile Glu Gln Asp Asp Gln Thr 
        915                 920                 925             


Ala Ile Phe Tyr Val Gln Lys Leu Asp Arg Ala Asn Gly Leu Phe Leu 
    930                 935                 940                 


Ala Pro His Leu Glu Ala Asn Ala Asp Ala Arg His Arg Asn Pro Asn 
945                 950                 955                 960 


Asp Ala Phe Lys Phe Leu Gln Met Gly Ala Gly Ser Val Val Arg Asn 
                965                 970                 975     


Lys Leu Arg Arg Val Tyr Val Asp Glu Ile Gly Arg Ile Lys Asp Pro 
            980                 985                 990         


Gly Pro Pro Lys His 
        995         


<210>  469
<211>  997
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  469

Met Lys Ile Leu Gly Phe Asp Ile Gly Ile Asn Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Glu Ala Glu Asn Pro Ser Asn Lys Glu Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Asn Ala Arg Ser Ser Arg Arg Arg Leu Lys Arg Arg Lys Ala Arg Leu 
    50                  55                  60                  


Val Ala Ile Lys Arg Ile Leu Ala Lys Glu Leu Lys Leu Asn Phe Lys 
65                  70                  75                  80  


Asp Tyr Val Ala Ala Asp Gly Glu Leu Pro Lys Ala Tyr Glu Gly Lys 
                85                  90                  95      


Leu Thr Ser Ile Tyr Glu Leu Arg Tyr Lys Ala Leu Ile Gln Gln Leu 
            100                 105                 110         


Glu Thr Lys Asp Leu Ala Arg Val Ile Leu His Ile Ala Lys His Arg 
        115                 120                 125             


Gly Tyr Met Asn Lys Asn Glu Lys Lys Ser Asn Asp Thr Lys Lys Gly 
    130                 135                 140                 


Lys Ile Leu Ser Ala Leu Lys Asn Asn Ala Leu Lys Leu Glu Asn Tyr 
145                 150                 155                 160 


Gln Ser Val Gly Glu Tyr Phe Tyr Lys Glu Phe Phe Gln Lys Tyr Lys 
                165                 170                 175     


Glu Asn Thr Lys Asn Phe Ile Lys Ile Arg Asn Thr Lys Asp Asn Tyr 
            180                 185                 190         


Asn Asn Cys Val Leu Ser Ser Asp Leu Glu Lys Glu Leu Lys Leu Ile 
        195                 200                 205             


Leu Glu Lys Gln Lys Glu Phe Gly Tyr Asn Tyr Ser Glu Asp Phe Ile 
    210                 215                 220                 


Asn Glu Ile Leu Lys Val Ala Phe Phe Gln Arg Pro Leu Lys Asp Phe 
225                 230                 235                 240 


Ser His Leu Val Gly Ala Cys Thr Phe Phe Glu Glu Glu Lys Arg Ala 
                245                 250                 255     


Cys Lys Asn Ser Tyr Ser Ala Trp Glu Phe Val Ala Leu Thr Lys Ile 
            260                 265                 270         


Ile Asn Glu Ile Lys Ser Leu Glu Lys Ile Ser Gly Glu Ile Val Pro 
        275                 280                 285             


Thr Gln Thr Ile Asn Glu Ile Leu Asn Leu Ile Leu Asp Lys Gly Ser 
    290                 295                 300                 


Ile Thr Tyr Lys Lys Phe Arg Ser Cys Ile Asn Leu His Glu Ser Ile 
305                 310                 315                 320 


Ser Phe Lys Ser Leu Lys Tyr Asp Lys Glu Asn Ala Glu Asn Ala Lys 
                325                 330                 335     


Leu Ile Asp Phe Arg Lys Leu Val Glu Phe Lys Lys Ala Leu Gly Val 
            340                 345                 350         


His Ser Leu Ser Arg Gln Glu Leu Asp Gln Ile Ser Thr His Ile Thr 
        355                 360                 365             


Leu Ile Lys Asp Asn Val Lys Leu Lys Thr Val Leu Glu Lys Tyr Asn 
    370                 375                 380                 


Leu Ser Asn Glu Gln Ile Asn Asn Leu Leu Glu Ile Glu Phe Asn Asp 
385                 390                 395                 400 


Tyr Ile Asn Leu Ser Phe Lys Ala Leu Gly Met Ile Leu Pro Leu Met 
                405                 410                 415     


Arg Glu Gly Lys Arg Tyr Tyr Glu Ala Cys Glu Ile Thr Asn Leu Lys 
            420                 425                 430         


Pro Lys Thr Val Asp Glu Lys Glu Asp Phe Leu Pro Ala Phe Cys Asp 
        435                 440                 445             


Ser Ile Phe Ala His Glu Leu Ser Asn Pro Val Val Asn Arg Ala Ile 
    450                 455                 460                 


Ser Glu Tyr Arg Lys Val Leu Asn Ala Leu Leu Lys Lys Tyr Gly Lys 
465                 470                 475                 480 


Val His Lys Ile His Leu Glu Leu Ala Arg Asp Val Gly Leu Ser Lys 
                485                 490                 495     


Lys Ala Arg Glu Lys Ile Glu Asp Gln Gln Lys Asn Asn Lys Ala Ile 
            500                 505                 510         


Asn Asp Trp Ala Leu Lys Glu Cys Glu Asn Ile Gly Ile Lys Ala Ser 
        515                 520                 525             


Ala Lys Asn Ile Leu Lys Leu Lys Leu Trp Lys Glu Gln Lys Glu Ile 
    530                 535                 540                 


Cys Ile Tyr Ser Gly Asn Lys Ile Ser Ile Glu His Leu Lys Asp Glu 
545                 550                 555                 560 


Lys Ala Leu Glu Val Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp 
                565                 570                 575     


Asp Ser Phe Ile Asn Lys Val Leu Val Phe Thr Lys Glu Asn Gln Glu 
            580                 585                 590         


Lys Leu Asn Lys Thr Pro Phe Glu Ala Phe Gly Lys Asn Ile Glu Lys 
        595                 600                 605             


Trp Ser Lys Ile Gln Thr Leu Ala Gln Asn Leu Pro Tyr Lys Lys Lys 
    610                 615                 620                 


Asn Lys Ile Leu Asp Glu Asn Phe Lys Asp Lys Gln Gln Gln Asp Phe 
625                 630                 635                 640 


Ile Ser Arg Asn Leu Asn Asp Thr Arg Tyr Ile Thr Thr Leu Ile Ala 
                645                 650                 655     


Lys Tyr Thr Lys Glu Tyr Leu Asn Phe Leu Pro Leu Ser Glu Asn Glu 
            660                 665                 670         


Asn Thr Asn Leu Lys Ser Gly Glu Lys Gly Ser Lys Ile His Ile Gln 
        675                 680                 685             


Thr Ile Ser Gly Met Leu Thr Ser Val Leu Arg His Thr Trp Gly Phe 
    690                 695                 700                 


Asp Lys Lys Asp Arg Asn Asn His Leu His His Ala Leu Asp Ala Ile 
705                 710                 715                 720 


Ile Val Ala Tyr Ser Thr Ser Ser Ile Ile Lys Ala Phe Ser Asp Phe 
                725                 730                 735     


Lys Lys Asn Gln Glu Leu Leu Lys Ala Arg Phe Tyr Ala Lys Glu Leu 
            740                 745                 750         


Thr Ser Asp Asn Tyr Lys His Gln Val Lys Phe Phe Glu Pro Phe Lys 
        755                 760                 765             


Gly Phe Arg Glu Lys Ile Leu Ser Lys Ile Asp Glu Ile Phe Val Ser 
    770                 775                 780                 


Thr Pro Pro Arg Lys Arg Ala Arg Gly Ala Leu His Glu Glu Thr Phe 
785                 790                 795                 800 


Tyr Ser Glu Asp Lys Met Ile Lys Lys Tyr Asn Ser Lys Glu Gly Leu 
                805                 810                 815     


Gln Ile Ala Leu Ser Cys Gly Arg Val Arg Lys Ile Gly Thr Lys Tyr 
            820                 825                 830         


Val Glu Asn Asp Thr Met Val Arg Val Asp Ile Phe Lys Lys Gln Asn 
        835                 840                 845             


Lys Phe Tyr Ala Ile Pro Ile Tyr Val Met Asp Phe Ala Leu Gly Ile 
    850                 855                 860                 


Leu Pro Asn Lys Ile Val Ile Thr Gly Lys Asp Lys Asn Asn Asn Pro 
865                 870                 875                 880 


Lys Gln Trp Gln Thr Ile Asp Glu Ser Tyr Glu Phe Cys Phe Ser Leu 
                885                 890                 895     


Tyr Lys Asn Asp Leu Ile Leu Leu Gln Lys Lys Asn Met Gln Glu Pro 
            900                 905                 910         


Glu Phe Ala Tyr Tyr Asn Ser Phe Asp Ile Asp Arg Ser Arg Ile Lys 
        915                 920                 925             


Ile Lys Lys His Asp Asn Lys Phe Glu Asn Leu Thr Ser Asn Gln Lys 
    930                 935                 940                 


Leu Leu Phe Thr Asn Lys Asp Lys Gly Lys Asn Arg Thr Gly Ile Gln 
945                 950                 955                 960 


Asn Leu Lys Ile Phe Glu Lys Tyr Ile Val Thr Pro Leu Gly Asp Lys 
                965                 970                 975     


Ile Lys Ala Asp Phe Gln Pro Arg Glu Asn Ile Ser Leu Lys Thr Ser 
            980                 985                 990         


Lys Lys His Gly Leu 
        995         


<210>  470
<211>  997
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  470

Met Gly Asn Tyr Thr Leu Gly Leu Asp Ile Gly Ser Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Val Glu Asp Ala Val Glu Ala Pro Arg Ile Val Asp Met 
            20                  25                  30          


Gly Val Arg Val Phe Pro Glu Gly Val Asp Arg Asp Thr Lys Gly Leu 
        35                  40                  45              


Glu Lys Ser Lys Asn Ala Thr Arg Arg Glu Ala Arg Gly Ala Arg Arg 
    50                  55                  60                  


Thr His Gln Arg Arg Arg Gln Arg Lys Gln Arg Leu Val Arg Ala Leu 
65                  70                  75                  80  


Arg Gln Val Gly Leu Leu Pro Cys Asp Lys Ala Glu Val Arg Ser Leu 
                85                  90                  95      


Phe Gln Thr Asp Pro Tyr Glu Ile Arg Lys Arg Gly Leu Asp His Arg 
            100                 105                 110         


Leu Glu Leu Tyr Glu Ile Gly Arg Ala Phe Tyr His Leu Ser Gln Arg 
        115                 120                 125             


Arg Gly Phe Lys Ser Asn Arg Lys Ser Gly Ala Leu Lys Glu Asp Gly 
    130                 135                 140                 


Thr Val Lys Lys Glu Ala Gly Glu Leu Gln Arg Lys Ile Asp Glu Ala 
145                 150                 155                 160 


Gly Cys Arg Thr Ile Gly Glu Tyr Phe Ala Gly Leu Asp Pro Glu Gln 
                165                 170                 175     


Gln Arg Ile Arg Gly Arg Tyr Thr Phe Arg Ser Met Tyr Gln Lys Glu 
            180                 185                 190         


Phe Asp Leu Leu Trp Ala Lys Gln Val Glu Phe Tyr Pro Lys Val Leu 
        195                 200                 205             


Thr Glu Asp Leu Arg Ser Lys Val Arg Asp Glu Ile Ile Phe Phe Gln 
    210                 215                 220                 


Arg Pro Leu Lys Ser Ala Lys Asp Leu Ile Gly Asp Cys Glu Leu Glu 
225                 230                 235                 240 


Pro Thr Glu Lys Arg Cys Pro Arg Ala Asp Trp Tyr Ala Arg Arg Phe 
                245                 250                 255     


Arg Leu Leu Gln Asp Ile Asn Asn Leu Ile Ile Gln Asn Pro Asp Gly 
            260                 265                 270         


Thr Glu Gln Lys Leu Ser Pro Asp Gln Arg Ala Ala Leu Leu Gly Glu 
        275                 280                 285             


Leu Gly Gln Arg Glu Lys Leu Thr Phe Asp Asn Ile Arg Ala Lys Leu 
    290                 295                 300                 


Gly Leu Leu Glu Thr Gln Lys Phe Asn Ala Glu Tyr Arg Ile Gly Arg 
305                 310                 315                 320 


Lys Gly Lys Lys Val Glu Ala Ile Lys Gly Asp Val Phe Val Trp Thr 
                325                 330                 335     


Met Arg Gly Lys Ser Val Phe Gly Pro Lys Ala Trp Asp Ala Met Ala 
            340                 345                 350         


Asp Glu Arg Lys Val Ser Leu Asn Glu Leu Phe Val Glu Leu Glu Asp 
        355                 360                 365             


Asp Asp Leu Ala Arg Lys Leu Glu Thr Glu Tyr Gly Leu Thr Asp Lys 
    370                 375                 380                 


Gln Val Gln Ala Ala Met Lys Ala Ser Leu Pro Gln Gly Tyr Met Ser 
385                 390                 395                 400 


Phe Ser Arg Lys Ala Val Leu Lys Leu Leu Pro Phe Met Glu Ala Gly 
                405                 410                 415     


Ala Leu Thr His Glu Ala Ile Asp Gln Ala Gly Tyr Arg Lys Glu Glu 
            420                 425                 430         


Pro Thr Gln Thr Asp Ala Ile Ala Arg Val Ser Phe Pro Pro Asp Leu 
        435                 440                 445             


Arg Asn Pro Ile Val Gln Lys Ala Leu Phe Glu Val Arg Lys Val Val 
    450                 455                 460                 


Asn Ala Leu Val Arg Glu Tyr Gly Lys Pro Thr Arg Ile Lys Ile Glu 
465                 470                 475                 480 


Met Ala Arg Asp Val Gln Gly Ser Lys Arg Gln Arg Glu Glu Leu His 
                485                 490                 495     


Trp Arg Asn Glu Asp Asn Arg Lys Lys Asn Glu Glu Val Arg Asp Arg 
            500                 505                 510         


Leu Arg Thr Asp Ile His Ile Asp Asp Pro Ser Arg Asp Asp Val Ile 
        515                 520                 525             


Lys Tyr Lys Leu Trp Gln Glu Ser Gly Lys Met Cys Pro Tyr Thr Gly 
    530                 535                 540                 


Arg Gln Ile Cys Gln Glu Ala Leu Phe Gly Pro Asn Pro Glu Phe Gln 
545                 550                 555                 560 


Val Glu His Ile Leu Pro Tyr Asp Arg Ser Leu Asp Asp Ser Tyr Met 
                565                 570                 575     


Asn Leu Thr Leu Cys Glu Val His Glu Asn Ile His Gly Lys Arg Asn 
            580                 585                 590         


Gln Thr Pro Phe Glu Ala Tyr Gly Ser Gly Ala Glu Arg Tyr Glu Gln 
        595                 600                 605             


Ile Lys Gln Arg Ile Lys Val Leu Pro Trp Pro Lys Arg Gln Lys Phe 
    610                 615                 620                 


Leu Gln Gln Glu Ile Asn Leu Asp Ser His Ile Ser Arg Glu Leu Asn 
625                 630                 635                 640 


Asp Thr Arg Tyr Ile Cys Arg Glu Thr Val Ala Tyr Leu Lys Gln Leu 
                645                 650                 655     


Gly Val His Val Arg Gly Thr Arg Gly Lys Ile Thr Ala Glu Leu Arg 
            660                 665                 670         


His Gln Trp Gly Leu Asp Gly Ile Phe Ser Glu Leu Gly Val Arg Arg 
        675                 680                 685             


Asp Asp Asp His Arg Arg His Ala Val Asp Ala Leu Val Val Ala Val 
    690                 695                 700                 


Thr Asp Asn Glu His Leu Arg Arg Leu Ala Val Ser Lys Tyr Val Leu 
705                 710                 715                 720 


Ala Gly Pro Arg Phe Glu Thr Pro Trp Ser Ala Phe Arg Asp Glu Ala 
                725                 730                 735     


Arg Gly Asn Val Lys Arg Met Asn Val Ser His Arg Val Cys Arg Lys 
            740                 745                 750         


Val Ser Gly Ala Leu His Glu Glu Thr Asn Tyr Gly Pro Thr Gly Val 
        755                 760                 765             


Arg Asp Gln Ala Gly Gln Asp Ile Tyr Ala Tyr Arg Lys Lys Leu Glu 
    770                 775                 780                 


Asp Leu Thr Thr Pro Met Val Glu Lys Ile Val Asp Pro Val Val Arg 
785                 790                 795                 800 


Asp Ile Val Arg Gln Arg Leu Leu Met Lys Gly Ile Asp Pro Ala Ala 
                805                 810                 815     


Ala Asp Arg Lys Ile Pro Lys Asp Val Trp Lys Glu Pro Leu Tyr Met 
            820                 825                 830         


Arg Arg Thr Lys Ser Asp Lys Met Ile Gln Ile Arg Arg Val Arg Ile 
        835                 840                 845             


His Asp Val Lys Asn Ser Ile Ile Val Leu Thr Asp Glu Ser Gly Arg 
    850                 855                 860                 


Pro Tyr Arg Ala Val Glu Pro Gly Gly Asn His His Val Glu Val Phe 
865                 870                 875                 880 


Glu Tyr Val His Gly Thr Lys Thr Gly Thr Arg Glu Ala Glu Val Val 
                885                 890                 895     


Thr Met Phe Glu Ala Val Arg Arg Ser Arg Ala Arg Ala Pro Val Val 
            900                 905                 910         


Arg Arg Asp His Gly Pro Gly Thr Arg Phe Val Cys Ser Leu Ala Ile 
        915                 920                 925             


Asn Asp Met Val Met Met Pro Asp Lys Ser Gly Lys Met Asp Leu Phe 
    930                 935                 940                 


Arg Ile Gln Lys Met Ser Ala Ser Lys Gln Val Tyr Phe Arg His His 
945                 950                 955                 960 


Thr Ala Ala Arg Ile Asp Ser Glu Arg Thr Leu Ile Arg Lys Gln Ala 
                965                 970                 975     


His Leu Phe Asn Gly Tyr Lys Val Ser Val Asp Pro Thr Gly Ala Ile 
            980                 985                 990         


Cys Arg Ala Asn Asp 
        995         


<210>  471
<211>  999
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  471

Met Lys Ile Leu Gly Phe Asp Ile Gly Ile Asn Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Asp Asn Gln Leu Gln Asp Cys Gly Val Arg Leu Phe Thr 
            20                  25                  30          


Lys Ala Glu Asp Pro Lys Thr Lys Glu Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Asn Ala Arg Ser Asn Arg Arg Arg Leu Gly Arg Arg Arg Ser Arg Leu 
    50                  55                  60                  


Ile Ala Leu Lys His Ile Ile Ser Lys Gly Leu Lys Leu Asn Tyr Gln 
65                  70                  75                  80  


Asp Tyr Ile Ala Asn Asp Gly Glu Leu Pro Lys Ala Tyr Glu Gly Arg 
                85                  90                  95      


Leu Ile Ser Pro Tyr Glu Leu Arg Tyr Lys Ala Leu Asn Glu Lys Ile 
            100                 105                 110         


Glu Pro Lys Asp Leu Ala Arg Val Ile Leu His Ile Ala Lys His Arg 
        115                 120                 125             


Gly Tyr Met Asn Lys Asn Glu Lys Lys Ser Ser Asp Asn Glu Lys Gly 
    130                 135                 140                 


Lys Ile Leu Ser Ala Leu Lys Thr Asn Ala Leu Lys Leu Glu Lys Tyr 
145                 150                 155                 160 


Gln Ser Val Gly Glu Tyr Phe Tyr Lys Glu Phe Phe Gln Lys Tyr Arg 
                165                 170                 175     


Glu Asn Thr Lys Asp Phe Ile Asn Ile Arg Asn Lys Glu Gly Ser Tyr 
            180                 185                 190         


Glu Asn Cys Val Leu Ala Ser Asp Leu Glu Lys Glu Leu Lys Leu Ile 
        195                 200                 205             


Leu Glu Lys Gln Lys Glu Trp Gly Tyr Ser Tyr Asn Asp Asn Phe Ile 
    210                 215                 220                 


Lys Glu Ile Leu Lys Val Ala Phe Phe Gln Arg Pro Leu Lys Asp Phe 
225                 230                 235                 240 


Ser Tyr Leu Val Gly Ala Cys Thr Phe Phe Glu Asp Glu Lys Arg Ala 
                245                 250                 255     


Cys Lys Asn Ser Tyr Ser Ala Trp Glu Phe Val Ala Leu Thr Lys Ile 
            260                 265                 270         


Ile Asn Glu Leu Lys Ser Leu Glu Lys Glu Ser Gly Glu Leu Val Ser 
        275                 280                 285             


Ser Gln Ile Ile Asn Glu Ile Leu Asn His Ile Leu Asp Lys Gly Ser 
    290                 295                 300                 


Ile Thr Tyr Lys Lys Phe Arg Glu Tyr Ile Lys Leu His Glu Ser Met 
305                 310                 315                 320 


Lys Phe Lys Ser Leu Lys Tyr Asp Lys Asp Asn Val Glu Ser Thr Lys 
                325                 330                 335     


Leu Ile Glu Phe Arg Lys Leu Val Glu Phe Lys Lys Ala Leu Gly Glu 
            340                 345                 350         


His Ser Leu Ser Arg Glu Glu Leu Asp Gln Ile Ala Thr Tyr Ile Thr 
        355                 360                 365             


Leu Ile Lys Asp Asn Glu Lys Leu Lys Ile Thr Leu Glu Lys Tyr Ser 
    370                 375                 380                 


Leu Asn Asn Glu Gln Ile Lys Asn Leu Leu Glu Ile Asp Phe Asn Asp 
385                 390                 395                 400 


His Ile Asn Leu Ser Phe Lys Ala Leu Asn Leu Ile Leu Pro Leu Met 
                405                 410                 415     


Lys Glu Gly Lys Arg Tyr Asp Glu Ala Cys Lys Leu Leu Asn Leu Lys 
            420                 425                 430         


Thr Lys Ser Asn Asn Gln Lys Phe Asp Phe Leu Pro Ala Phe Cys Asp 
        435                 440                 445             


Ser Ile Phe Ala Gln Glu Leu Thr Asn Pro Ile Val Asn Arg Ala Ile 
    450                 455                 460                 


Ser Glu Tyr Arg Lys Val Leu Asn Ala Leu Leu Lys Lys Tyr Gly Lys 
465                 470                 475                 480 


Met His Lys Ile His Ile Glu Leu Ala Arg Asp Ile Gly Leu Ser Lys 
                485                 490                 495     


Lys Leu Arg Ala Lys Ile Glu Lys Glu Gln Lys Glu Asn Tyr Glu Asn 
            500                 505                 510         


Asn Ile Trp Ala Leu Asn Glu Cys Glu Lys Phe Gly Leu Lys Ala Asn 
        515                 520                 525             


Ala Lys Asn Ile Leu Lys Leu Lys Leu Trp Lys Glu Gln Lys Glu Phe 
    530                 535                 540                 


Cys Ile Tyr Ser Gly Lys Lys Ile Ser Ile Glu His Leu Arg Asp Glu 
545                 550                 555                 560 


Lys Ala Leu Glu Val Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp 
                565                 570                 575     


Asp Ser Phe Leu Asn Lys Val Leu Val Phe Thr Lys Glu Asn Gln Glu 
            580                 585                 590         


Lys Leu Asn Lys Thr Pro Phe Glu Ala Phe Gly Ala Asn Glu Glu Arg 
        595                 600                 605             


Trp Ser Lys Ile Gln Ala Leu Ala Gln Asn Leu Pro Tyr Lys Lys Lys 
    610                 615                 620                 


Asn Lys Ile Leu Asp Glu Ala Phe Lys Gly Lys Gln Gln Gln Asp Phe 
625                 630                 635                 640 


Ile Ser Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Thr Leu Ile Val 
                645                 650                 655     


Lys Tyr Thr Lys Glu Tyr Leu Asp Phe Leu Pro Leu Asp Glu Lys Glu 
            660                 665                 670         


Asp Val Asn Leu Lys Ser Gly Glu Lys Gly Ser Lys Ile His Val Gln 
        675                 680                 685             


Thr Ile Asn Gly Met Leu Thr Ser Val Leu Arg His Thr Trp Gly Phe 
    690                 695                 700                 


Ala Gln Lys Asp Arg Asn Asn His Leu His His Ala Leu Asp Ala Thr 
705                 710                 715                 720 


Ile Val Ala Tyr Ser Thr Asn Ala Ile Ile Lys Ala Phe Ser Asp Phe 
                725                 730                 735     


Lys Lys Glu Gln Glu Leu Leu Lys Ala Lys Leu Tyr Ala Lys Glu Leu 
            740                 745                 750         


Thr Ser Asp Ser Tyr Lys His Gln Ala Lys Phe Phe Glu Pro Phe Glu 
        755                 760                 765             


Gly Phe Arg Glu Gln Ile Leu Asn Gln Ile Asn Lys Leu Phe Val Ser 
    770                 775                 780                 


Lys Pro Pro Arg Lys Arg Ala Arg Gly Ala Leu His Lys Glu Thr Phe 
785                 790                 795                 800 


Tyr Ser Lys Asp Glu Met Ile Lys Lys Tyr Asn Ser Gln Glu Gly Val 
                805                 810                 815     


Glu Ile Ala Leu Asn Cys Gly Lys Ile Arg Lys Ile Gly Thr Lys Tyr 
            820                 825                 830         


Val Glu Asn Asp Thr Met Val Arg Ile Asp Ile Phe Lys Lys Gln Asn 
        835                 840                 845             


Lys Phe Tyr Ala Ile Pro Ile Tyr Thr Met Asp Phe Ala Leu Gly Val 
    850                 855                 860                 


Leu Pro Asn Lys Ile Val Ile Ile Gly Lys Asp Lys Lys Gly Asn Pro 
865                 870                 875                 880 


Lys Gln Trp Gln Glu Ile Asp Glu Ser Tyr Glu Phe Cys Phe Ser Leu 
                885                 890                 895     


His Lys Asp Asp Leu Val Leu Ile Gln Lys Lys Asp Met Gln Glu Pro 
            900                 905                 910         


Glu Phe Ala Tyr Tyr Asn Gly Phe Asp Ile Ser Asn Ser Ser Ile Cys 
        915                 920                 925             


Val Glu Lys His Asp Asn Lys Phe Glu Asn Leu Thr Asp Asn Gln Arg 
    930                 935                 940                 


Leu Leu Phe Thr Asn Ala Glu Gly Asn Val Lys Ala Lys Lys Ile Gly 
945                 950                 955                 960 


Ile Gln Gly Leu Arg Ile Phe Glu Lys Tyr Ile Ile Thr Pro Leu Gly 
                965                 970                 975     


Glu Lys Ile Lys Ala Asp Phe Lys Pro Arg Glu Asp Ile Ala Leu Lys 
            980                 985                 990         


Thr Ser Lys Lys His Gly Leu 
        995                 


<210>  472
<211>  1000
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  472

Met Lys Ile Leu Gly Phe Asp Ile Gly Ile Asn Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Asn Lys Glu Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Asn Ala Arg Ser Ser Arg Arg Arg Leu Lys Arg Arg Lys Ala Arg Leu 
    50                  55                  60                  


Ile Ala Ile Lys Arg Ile Leu Ala Lys Glu Leu Lys Leu Asn Phe Lys 
65                  70                  75                  80  


Asp Tyr Val Ala Ala Asp Gly Glu Leu Pro Lys Ala Tyr Glu Gly Ser 
                85                  90                  95      


Leu Ala Ser Val Tyr Glu Leu Arg Tyr Lys Ala Leu Thr Gln Lys Leu 
            100                 105                 110         


Glu Thr Lys Asp Leu Ala Arg Val Ile Leu His Ile Ala Lys His Arg 
        115                 120                 125             


Gly Tyr Met Asn Lys Asn Glu Lys Lys Ser Asn Asp Ala Lys Lys Gly 
    130                 135                 140                 


Lys Ile Leu Ser Ala Leu Lys Asn Asn Ala Leu Lys Leu Glu Asn Tyr 
145                 150                 155                 160 


Gln Ser Val Gly Glu Tyr Phe Tyr Lys Glu Phe Phe Gln Lys Tyr Lys 
                165                 170                 175     


Glu Asn Thr Lys Asn Phe Ile Lys Ile Arg Asn Thr Lys Asp Asn Tyr 
            180                 185                 190         


Asn Asn Cys Val Leu Ser Ser Asp Leu Glu Lys Glu Leu Lys Phe Ile 
        195                 200                 205             


Leu Glu Lys Gln Lys Glu Phe Gly Tyr Lys Tyr Ser Glu Gly Phe Ile 
    210                 215                 220                 


Asn Glu Ile Leu Lys Val Ala Phe Phe Gln Arg Pro Leu Lys Asp Phe 
225                 230                 235                 240 


Ser His Leu Val Gly Ala Cys Thr Phe Phe Glu Glu Glu Asn Arg Ala 
                245                 250                 255     


Cys Lys Asn Ser Tyr Ser Ala Trp Glu Phe Val Ala Leu Thr Lys Ile 
            260                 265                 270         


Ile Asn Glu Ile Lys Ser Leu Glu Lys Ile Ser Gly Glu Ile Val Ser 
        275                 280                 285             


Thr Gln Thr Ile Asn Glu Val Leu Asn Leu Ile Leu Asp Lys Gly Ser 
    290                 295                 300                 


Ile Thr Tyr Lys Lys Phe Arg Ser Cys Ile Asn Leu His Glu Ser Ile 
305                 310                 315                 320 


Ser Phe Lys Ser Leu Lys Tyr Asp Lys Glu Asn Ala Glu Asn Ala Lys 
                325                 330                 335     


Leu Ile Asp Phe Arg Lys Leu Val Glu Phe Lys Lys Ala Leu Gly Val 
            340                 345                 350         


His Ser Leu Ser Arg Gln Glu Leu Asp Gln Ile Ser Thr His Val Thr 
        355                 360                 365             


Leu Ile Lys Asp Asn Val Lys Leu Lys Thr Val Leu Glu Lys Tyr Asn 
    370                 375                 380                 


Leu Ser Asn Glu Gln Ile Asn Asn Leu Leu Glu Ile Glu Phe Asn Asp 
385                 390                 395                 400 


Tyr Ile Asn Leu Ser Phe Lys Ala Leu Gly Met Ile Leu Pro Leu Met 
                405                 410                 415     


Arg Glu Gly Lys Arg Tyr Asp Glu Ala Cys Glu Ile Thr Asn Leu Lys 
            420                 425                 430         


Pro Lys Thr Val Asp Glu Lys Lys Asp Phe Leu Pro Ala Phe Cys Asp 
        435                 440                 445             


Ser Ile Phe Ala His Glu Leu Ser Asn Pro Val Val Asn Arg Ala Ile 
    450                 455                 460                 


Ser Glu Tyr Arg Lys Val Leu Asn Ala Leu Leu Lys Lys Tyr Asp Lys 
465                 470                 475                 480 


Val His Lys Ile His Leu Glu Leu Ala Arg Asp Val Gly Leu Ser Lys 
                485                 490                 495     


Lys Ala Arg Glu Lys Ile Glu Lys Glu Gln Lys Glu Asn Gln Ala Val 
            500                 505                 510         


Asn Ala Trp Ala Leu Lys Glu Cys Glu Asn Ile Gly Leu Lys Ala Ser 
        515                 520                 525             


Ala Lys Asn Ile Leu Lys Leu Lys Leu Trp Lys Glu Gln Lys Glu Ile 
    530                 535                 540                 


Cys Ile Tyr Ser Gly Asn Lys Ile Ser Ile Glu His Leu Lys Asp Glu 
545                 550                 555                 560 


Lys Ala Leu Glu Val Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp 
                565                 570                 575     


Asp Ser Phe Met Asn Lys Val Leu Val Phe Thr Lys Glu Asn Gln Glu 
            580                 585                 590         


Lys Leu Asn Lys Thr Pro Phe Glu Ala Phe Gly Lys Asn Ile Glu Lys 
        595                 600                 605             


Trp Ser Lys Ile Gln Thr Leu Ala Gln Asn Leu Pro Tyr Lys Lys Lys 
    610                 615                 620                 


Asn Lys Ile Leu Asp Glu Asn Phe Lys Asp Lys Gln Gln Glu Asp Phe 
625                 630                 635                 640 


Ile Ser Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ala Thr Leu Ile Thr 
                645                 650                 655     


Lys Tyr Thr Lys Glu Tyr Leu Asn Phe Leu Pro Leu Asn Glu Asn Glu 
            660                 665                 670         


Asn Thr Asn Leu Lys Ser Gly Glu Lys Gly Ser Lys Ile His Val Gln 
        675                 680                 685             


Thr Val Asn Gly Met Leu Thr Ser Val Leu Arg His Thr Trp Gly Phe 
    690                 695                 700                 


Asp Lys Lys Asp Arg Asn Asn His Leu His His Ala Leu Asp Ala Ile 
705                 710                 715                 720 


Ile Val Ala Tyr Ser Thr Asn Ser Ile Ile Lys Ala Phe Ser Asp Phe 
                725                 730                 735     


Lys Lys Asn Gln Glu Leu Leu Lys Ala Arg Phe Tyr Ala Lys Glu Leu 
            740                 745                 750         


Thr Ser Asp Asn Tyr Lys His Gln Val Lys Phe Phe Glu Pro Phe Lys 
        755                 760                 765             


Gly Phe Arg Glu Lys Ile Leu Ser Lys Ile Asp Glu Ile Phe Val Ser 
    770                 775                 780                 


Lys Pro Pro Arg Lys Arg Ala Arg Gly Ala Leu His Lys Glu Thr Phe 
785                 790                 795                 800 


Tyr Ser Glu Asp Glu Met Ile Lys Lys Tyr Asn Ser Lys Glu Gly Leu 
                805                 810                 815     


Gln Ile Ala Leu Ser Cys Gly Arg Val Arg Lys Ile Gly Thr Lys Tyr 
            820                 825                 830         


Val Glu Asn Asp Thr Met Val Arg Val Asp Ile Phe Lys Lys Gln Asn 
        835                 840                 845             


Lys Phe Tyr Ala Ile Pro Ile Tyr Ala Met Asp Phe Ala Leu Gly Ile 
    850                 855                 860                 


Leu Pro Asn Lys Ile Val Val Val Gly Lys Asp Lys Asn Asn Asn Pro 
865                 870                 875                 880 


Lys Gln Trp Gln Thr Ile Asp Glu Ser Tyr Glu Phe Cys Phe Ser Leu 
                885                 890                 895     


Tyr Lys Asn Asp Leu Ile Met Leu Gln Lys Lys Asp Met Gln Glu Ala 
            900                 905                 910         


Glu Phe Ala Tyr Tyr Asn Gly Phe Asp Ile Ser Thr Ser Ser Ile Cys 
        915                 920                 925             


Val Glu Lys His Asp Ser Lys Phe Glu Asn Leu Thr Ser Asn Gln Lys 
    930                 935                 940                 


Leu Leu Phe Ser Asn Ala Lys Glu Gly Ser Val Lys Val Glu Asn Leu 
945                 950                 955                 960 


Gly Ile Gln Asn Leu Lys Ile Phe Glu Lys Tyr Ile Ile Thr Pro Leu 
                965                 970                 975     


Gly Asp Lys Ile Lys Ala Asp Phe Gln Pro Arg Glu Asn Ile Ser Leu 
            980                 985                 990         


Lys Thr Ser Lys Lys Asn Gly Leu  
        995                 1000 


<210>  473
<211>  1000
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  473

Met Ala Arg Ala Gln Arg Arg Leu Ile Ser Arg Arg Ala Asn Arg Met 
1               5                   10                  15      


Asn Lys Leu Arg Arg Leu Leu Lys Gln Glu Asn Val Leu Gln Ala Asn 
            20                  25                  30          


Asp Tyr Asp Glu Lys Gly Leu Ile Ile Gly Leu Pro Asn Gln Ala Trp 
        35                  40                  45              


Glu Leu Arg Ala Gln Gly Leu Asp Arg Lys Leu Glu Pro Lys Glu Trp 
    50                  55                  60                  


Ala Ala Val Leu Ile Asn Leu Val Lys His Arg Gly Tyr Leu Ser Gln 
65                  70                  75                  80  


Arg Lys Asn Glu Ser Gln Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu 
                85                  90                  95      


Ser Gly Met Asn His Asn His Gln Leu Leu Gln Ser Gln Ser Asn Gln 
            100                 105                 110         


Tyr Arg Thr Pro Ala Glu Leu Ala Ile Lys Gln Phe Ala Thr Gln Asp 
        115                 120                 125             


Gly His Ile Arg Asn Gln Arg Gly Ala Tyr Thr His Thr Phe Asn Arg 
    130                 135                 140                 


Leu Asp Leu Gln Asn Glu Leu Asn Gln Leu Phe Asp Ala Gln Thr Arg 
145                 150                 155                 160 


Leu Gly Asn Pro His Val Ser Gly Ser Leu Lys Thr Ala Ile Asn Asp 
                165                 170                 175     


Leu Leu Met Asn Gln Lys Ala Ala Leu Ser Gly Asp Ala Ile Leu Glu 
            180                 185                 190         


Met Leu Gly Arg Cys Thr Phe Glu Pro Ser Glu Tyr Lys Ala Ala Lys 
        195                 200                 205             


Asn Thr Tyr Ser Ala Glu Arg Phe Val Trp Leu Gly Lys Leu Thr Asn 
    210                 215                 220                 


Leu Arg Ile Gln Asp Asn Gly Leu Glu Ser Ala Leu Ser Arg Glu Gln 
225                 230                 235                 240 


Arg Glu Leu Leu Leu Asn Glu Pro Tyr Asn Lys Thr Lys Leu Thr Tyr 
                245                 250                 255     


Gln Gln Val Arg Lys Leu Leu Arg Leu Pro Glu Thr Ala Phe Phe Lys 
            260                 265                 270         


Gly Leu Arg Tyr Gly Lys Asp Asn Ala Glu Ser Ala Thr Phe Met Glu 
        275                 280                 285             


Met Lys Ser Tyr His Ala Ile Arg Lys Ser Leu Glu Asn Lys Ser Leu 
    290                 295                 300                 


Lys Ser Glu Trp Glu Lys Leu Lys Thr Gln Pro Lys Leu Leu Asp Leu 
305                 310                 315                 320 


Ile Gly Thr Ala Phe Ser Ile Tyr Lys Thr Asp Asp Glu Ile Ala Gly 
                325                 330                 335     


Cys Leu Lys Asn Gln Leu Pro Glu Asn Val Ile Ile Ala Leu Gln Glu 
            340                 345                 350         


Gln Leu Asn Phe Ser Asn Phe Met Glu Leu Ser Leu Lys Ala Leu Glu 
        355                 360                 365             


Lys Leu Leu Pro Leu Met Glu Gln Gly Leu Arg Tyr Asp Glu Ala Cys 
    370                 375                 380                 


Thr Gln Ile Tyr Gly Asn Arg His Glu Lys Ile Ala Asp Lys Thr Glu 
385                 390                 395                 400 


Lys Phe Leu Pro Asn Ile Pro Ala Asp Lys Leu Arg Asn Pro Val Val 
                405                 410                 415     


Leu Arg Thr Leu Thr Gln Ala Arg Lys Val Ile Asn Ala Ile Ile Arg 
            420                 425                 430         


Arg Tyr Gly Ser Pro Ala Arg Val His Ile Glu Thr Gly Arg Glu Val 
        435                 440                 445             


Gly Lys Ser Phe Ser Asp Arg Asp Lys Leu Lys Lys Gln Gln Glu Glu 
    450                 455                 460                 


Asn Gln Glu Gln Arg Arg Arg Ala Ile Asp Glu Trp Lys Gln Phe Phe 
465                 470                 475                 480 


Pro Asn Ala Ala His Glu Pro Lys Ala Val Asp Ile Leu Lys Leu Arg 
                485                 490                 495     


Leu Tyr Gln Leu Gln Gln Gly Lys Cys Leu Tyr Ser Gly Leu Pro Ile 
            500                 505                 510         


Asp Val Arg Arg Leu Pro Glu Lys Gly Tyr Val Glu Ile Asp His Ala 
        515                 520                 525             


Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Ile Leu 
    530                 535                 540                 


Val Leu Ala Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu 
545                 550                 555                 560 


Tyr Leu Arg Gly Ala Asp Asn Ser Glu Ser Trp Arg Leu Phe Val Glu 
                565                 570                 575     


Asn Val Gln Asn Cys Gln Phe Ser Pro Asn Lys Lys Gln Lys Ile Met 
            580                 585                 590         


Ala Lys Gln Leu Asp Glu Lys Gly Phe Leu Glu Arg Asn Leu Asn Asp 
        595                 600                 605             


Thr Arg Tyr Val Ser Arg Phe Leu Cys Gln Phe Ile Ser Glu His Leu 
    610                 615                 620                 


His Leu Thr Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln 
625                 630                 635                 640 


Ile Thr Ala Leu Leu Arg Gly Arg Trp Gly Leu Ser Lys Asn Arg Glu 
                645                 650                 655     


Glu Asn Asp Arg His His Ala Val Asp Ala Val Val Val Ala Cys Ser 
            660                 665                 670         


Thr Val Ala Met Gln Gln Lys Ile Thr Gln Phe Val Arg Tyr Gln Lys 
        675                 680                 685             


Gly Asn Val Phe Thr Gly Glu Arg Ile Asp Lys Glu Thr Gly Glu Val 
    690                 695                 700                 


Ile Ser Ile His Phe Pro Thr Pro Trp Glu His Phe Arg Glu Glu Val 
705                 710                 715                 720 


Met Ile Arg Val Phe Asp Asp Asn Pro Leu Arg Thr Leu Pro Glu Lys 
                725                 730                 735     


Leu Pro Asn Arg Pro Glu Ala Leu His Glu Tyr Val Met Pro Leu Phe 
            740                 745                 750         


Val Ser Arg Met Pro Thr Arg Lys Met Thr Gly Gln Gly His Lys Glu 
        755                 760                 765             


Thr Ile Lys Ser Ala Lys Arg Leu Asp Glu Lys Ile Ser Val Leu Arg 
    770                 775                 780                 


Val Pro Leu Thr Gln Leu Lys Leu Asn Asp Leu Glu Asn Met Val Asn 
785                 790                 795                 800 


Arg Glu Arg Glu Pro Ala Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu 
                805                 810                 815     


Gln Phe Lys Asn Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Phe Lys 
            820                 825                 830         


Gln Gly Gly Gln Gln Val Lys Ala Val Arg Val Glu Ile Val Gln Lys 
        835                 840                 845             


Ser Gly Met Leu Leu Arg Lys Ile Asn Gly Val Ala Asp Asn Gly Ser 
    850                 855                 860                 


Met Val Arg Val Asp Val Phe Glu Lys Ser Gly Lys Phe Tyr Leu Val 
865                 870                 875                 880 


Pro Ile Tyr Ala Trp Gln Val Ala Glu Gly Ile Leu Pro Asn Tyr Ala 
                885                 890                 895     


Val Val Ala His Lys Asp Glu Ser Asp Trp Ile Leu Met Asp Glu Ser 
            900                 905                 910         


Tyr His Phe Lys Phe Ala Leu Tyr Lys Asn Asp Leu Leu Glu Val Gln 
        915                 920                 925             


Thr Lys Lys Gly Gly Ile Met Gly Tyr Phe Ala Ser Leu Asp Arg Ala 
    930                 935                 940                 


Thr Gly Ala Ile Thr Ile Arg Glu His Asp Arg Asp Lys Lys Lys Cys 
945                 950                 955                 960 


Lys Asp Gly Glu His Arg Ser Ile Gly Val Lys Met Ala Ser Ser Phe 
                965                 970                 975     


Lys Lys Leu Gln Val Asp Glu Leu Gly Lys Asn Ile His Glu Thr Arg 
            980                 985                 990         


Ser Arg Lys Arg Pro Asn Ile Gly  
        995                 1000 


<210>  474
<211>  1003
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  474

Met Arg Ile Leu Gly Phe Asp Ile Gly Ile Asn Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Asn Lys Glu Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Asn Ala Arg Ser Ser Arg Arg Arg Leu Lys Arg Arg Lys Ala Arg Leu 
    50                  55                  60                  


Ile Ala Ile Lys Arg Ile Leu Ala Lys Glu Leu Lys Leu Asn Tyr Lys 
65                  70                  75                  80  


Asp Tyr Val Ala Ala Asp Gly Glu Leu Pro Lys Ala Tyr Glu Gly Ser 
                85                  90                  95      


Leu Ala Ser Val Tyr Glu Leu Arg Tyr Lys Ala Leu Thr Gln Asn Leu 
            100                 105                 110         


Glu Thr Lys Asp Leu Ala Arg Val Ile Leu His Ile Ala Lys His Arg 
        115                 120                 125             


Gly Tyr Met Asn Lys Asn Glu Lys Lys Ser Asn Asp Ala Lys Lys Gly 
    130                 135                 140                 


Lys Ile Leu Ser Ala Leu Lys Asn Asn Ala Leu Lys Leu Glu Asn Tyr 
145                 150                 155                 160 


Gln Ser Val Gly Glu Tyr Phe Tyr Lys Glu Phe Phe Gln Lys Tyr Lys 
                165                 170                 175     


Lys Asn Thr Lys Asn Phe Ile Lys Ile Arg Asn Thr Lys Asp Asn Tyr 
            180                 185                 190         


Asn Asn Cys Val Leu Ser Ser Asp Leu Glu Lys Glu Leu Lys Leu Ile 
        195                 200                 205             


Leu Glu Lys Gln Lys Glu Phe Gly Tyr Asn Tyr Ser Glu Asp Phe Ile 
    210                 215                 220                 


Asn Glu Ile Leu Lys Val Ala Phe Phe Gln Arg Pro Leu Lys Asp Phe 
225                 230                 235                 240 


Ser His Leu Val Gly Ala Cys Thr Phe Phe Glu Glu Glu Lys Arg Ala 
                245                 250                 255     


Cys Lys Asn Ser Tyr Ser Ala Trp Glu Phe Val Ala Leu Thr Lys Ile 
            260                 265                 270         


Ile Asn Glu Ile Lys Ser Leu Glu Lys Ile Ser Gly Glu Ile Val Pro 
        275                 280                 285             


Thr Gln Thr Ile Asn Glu Val Leu Asn Leu Ile Leu Asp Lys Gly Ser 
    290                 295                 300                 


Ile Thr Tyr Lys Lys Phe Arg Ser Cys Ile Asn Leu His Glu Ser Ile 
305                 310                 315                 320 


Ser Phe Lys Ser Leu Lys Tyr Asp Lys Glu Asn Ala Glu Asn Ala Lys 
                325                 330                 335     


Leu Ile Asp Phe Arg Lys Leu Val Glu Phe Lys Lys Ala Leu Gly Val 
            340                 345                 350         


His Ser Leu Ser Arg Gln Glu Leu Asp Gln Ile Ser Thr His Ile Thr 
        355                 360                 365             


Leu Ile Lys Asp Asn Val Lys Leu Lys Thr Val Leu Glu Lys Tyr Asn 
    370                 375                 380                 


Leu Ser Asn Glu Gln Ile Asn Asn Leu Leu Glu Ile Glu Phe Asn Asp 
385                 390                 395                 400 


Tyr Ile Asn Leu Ser Phe Lys Ala Leu Gly Met Ile Leu Pro Leu Met 
                405                 410                 415     


Arg Glu Gly Lys Arg Tyr Asp Glu Ala Cys Glu Ile Ala Asn Leu Lys 
            420                 425                 430         


Pro Lys Thr Val Asp Glu Lys Lys Asp Phe Leu Pro Ala Phe Cys Asp 
        435                 440                 445             


Ser Ile Phe Ala His Glu Leu Ser Asn Pro Val Val Asn Arg Ala Ile 
    450                 455                 460                 


Ser Glu Tyr Arg Lys Val Leu Asn Ala Leu Leu Lys Lys Tyr Gly Lys 
465                 470                 475                 480 


Val His Lys Ile His Leu Glu Leu Ala Arg Asp Val Gly Leu Ser Lys 
                485                 490                 495     


Lys Ala Arg Glu Lys Ile Glu Lys Glu Gln Lys Glu Asn Gln Ala Val 
            500                 505                 510         


Asn Ala Trp Ala Leu Lys Glu Cys Glu Asn Ile Gly Leu Lys Ala Ser 
        515                 520                 525             


Ala Lys Asn Ile Leu Lys Leu Lys Leu Trp Lys Glu Gln Lys Glu Ile 
    530                 535                 540                 


Cys Ile Tyr Ser Gly Asn Lys Ile Ser Ile Glu His Leu Lys Asp Glu 
545                 550                 555                 560 


Lys Ala Leu Glu Val Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp 
                565                 570                 575     


Asp Ser Phe Ile Asn Lys Val Leu Val Phe Thr Lys Glu Asn Gln Glu 
            580                 585                 590         


Lys Leu Asn Lys Thr Pro Phe Glu Ala Phe Gly Lys Asn Ile Glu Lys 
        595                 600                 605             


Trp Ser Lys Ile Gln Thr Leu Ala Gln Asn Leu Pro Tyr Lys Lys Lys 
    610                 615                 620                 


Asn Lys Ile Leu Asp Glu Asn Phe Lys Asp Lys Gln Gln Glu Asp Phe 
625                 630                 635                 640 


Ile Ser Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ala Thr Leu Ile Ala 
                645                 650                 655     


Lys Tyr Thr Lys Glu Tyr Leu Asn Phe Leu Leu Leu Ser Glu Asn Glu 
            660                 665                 670         


Asn Ala Asn Leu Lys Ser Gly Glu Lys Gly Ser Lys Ile His Val Gln 
        675                 680                 685             


Thr Ile Ser Gly Met Leu Thr Ser Val Leu Arg His Thr Trp Gly Phe 
    690                 695                 700                 


Asp Lys Lys Asp Arg Asn Asn His Leu His His Ala Leu Asp Ala Ile 
705                 710                 715                 720 


Ile Val Ala Tyr Ser Thr Asn Ser Ile Ile Lys Ala Phe Ser Asp Phe 
                725                 730                 735     


Arg Lys Asn Gln Glu Leu Leu Lys Ala Arg Phe Tyr Ala Lys Glu Leu 
            740                 745                 750         


Thr Ser Asp Asn Tyr Lys His Gln Val Lys Phe Phe Glu Pro Phe Lys 
        755                 760                 765             


Ser Phe Arg Glu Lys Ile Leu Ser Lys Ile Asp Glu Ile Phe Val Ser 
    770                 775                 780                 


Lys Pro Pro Arg Lys Arg Ala Arg Arg Ala Leu His Lys Asp Thr Phe 
785                 790                 795                 800 


His Ser Glu Asn Lys Ile Ile Asp Lys Cys Ser Tyr Asn Ser Lys Glu 
                805                 810                 815     


Gly Leu Gln Ile Ala Leu Ser Cys Gly Arg Val Arg Lys Ile Gly Thr 
            820                 825                 830         


Lys Tyr Val Glu Asn Asp Thr Ile Val Arg Val Asp Ile Phe Lys Lys 
        835                 840                 845             


Gln Asn Lys Phe Tyr Ala Ile Pro Ile Tyr Ala Met Asp Phe Ala Leu 
    850                 855                 860                 


Gly Ile Leu Pro Asn Lys Ile Val Ile Thr Gly Lys Asp Lys Asn Asn 
865                 870                 875                 880 


Asn Pro Lys Gln Trp Gln Thr Ile Asp Glu Ser Tyr Glu Phe Cys Phe 
                885                 890                 895     


Ser Leu Tyr Lys Asn Asp Leu Ile Leu Leu Gln Lys Lys Asn Met Gln 
            900                 905                 910         


Glu Pro Glu Phe Ala Tyr Tyr Asn Asp Phe Ser Ile Ser Thr Ser Ser 
        915                 920                 925             


Ile Cys Val Glu Lys His Asp Asn Lys Phe Glu Asn Leu Thr Ser Asn 
    930                 935                 940                 


Gln Lys Leu Leu Phe Ser Asn Ala Lys Glu Gly Ser Val Lys Val Glu 
945                 950                 955                 960 


Ser Leu Gly Ile Gln Asn Leu Lys Val Phe Glu Lys Tyr Ile Ile Thr 
                965                 970                 975     


Pro Leu Gly Asp Lys Ile Lys Ala Asp Phe Gln Pro Arg Glu Asn Ile 
            980                 985                 990         


Ser Leu Lys Thr Ser Lys Lys Tyr  Gly Leu Arg 
        995                 1000             


<210>  475
<211>  1003
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  475

Met Ile Asp Glu Ser Leu Thr Phe Gly Ile Asp Leu Gly Ile Gly Ser 
1               5                   10                  15      


Cys Gly Trp Ala Val Leu Arg Arg Pro Ser Ala Phe Gly Arg Lys Gly 
            20                  25                  30          


Val Ile Glu Gly Met Gly Ser Trp Cys Phe Asp Val Pro Glu Thr Ser 
        35                  40                  45              


Lys Glu Arg Thr Pro Thr Asn Gln Ile Arg Arg Ser Asn Arg Leu Leu 
    50                  55                  60                  


Arg Arg Val Ile Arg Arg Arg Arg Asn Arg Met Ala Ala Ile Arg Arg 
65                  70                  75                  80  


Leu Leu His Ala Ala Gly Leu Leu Pro Ser Thr Asp Ser Asp Ala Leu 
                85                  90                  95      


Lys Arg Pro Gly His Asp Pro Trp Glu Leu Arg Ala Arg Gly Leu Asp 
            100                 105                 110         


Lys Pro Leu Lys Pro Val Glu Phe Ala Val Val Leu Gly His Ile Ala 
        115                 120                 125             


Lys Arg Arg Gly Phe Lys Ser Ala Ala Lys Arg Lys Ala Thr Asn Ile 
    130                 135                 140                 


Ser Ser Asp Asp Lys Lys Met Leu Thr Ala Leu Glu Ala Thr Arg Glu 
145                 150                 155                 160 


Arg Leu Gly Arg Tyr Arg Thr Val Gly Glu Met Phe Ala Arg Asp Pro 
                165                 170                 175     


Asp Phe Ala Ser Arg Arg Arg Asn Arg Glu Gly Lys Tyr Asp Arg Thr 
            180                 185                 190         


Thr Ala Arg Asp Asp Leu Glu His Glu Val His Ala Leu Phe Ala Ala 
        195                 200                 205             


Gln Arg Arg Leu Gly Gln Gly Phe Ala Ser Pro Glu Leu Glu Glu Ala 
    210                 215                 220                 


Phe Thr Ala Ser Ala Phe His Gln Arg Pro Met Gln Asp Ser Glu Arg 
225                 230                 235                 240 


Leu Val Gly Phe Cys Pro Phe Glu Arg Thr Glu Lys Arg Ala Ala Lys 
                245                 250                 255     


Leu Thr Pro Ser Phe Glu Arg Phe Arg Leu Leu Ala Arg Leu Leu Asn 
            260                 265                 270         


Leu Arg Ile Thr Thr Pro Asp Gly Glu Arg Pro Leu Thr Val Asp Glu 
        275                 280                 285             


Ile Ala Leu Val Thr Arg Asp Leu Gly Lys Thr Ala Lys Leu Ser Ile 
    290                 295                 300                 


Lys Arg Val Arg Thr Leu Ile Gly Leu Glu Asp Asn Gln Arg Phe Thr 
305                 310                 315                 320 


Thr Ile Arg Pro Glu Asp Glu Asp Arg Asp Ile Val Ala Arg Thr Gly 
                325                 330                 335     


Gly Ala Met Thr Gly Thr Ala Thr Leu Arg Lys Ala Leu Gly Glu Ala 
            340                 345                 350         


Leu Trp Thr Asp Met Gln Glu Arg Pro Glu Gln Leu Asp Ala Ile Val 
        355                 360                 365             


Gln Val Leu Ser Phe Phe Glu Ala Asn Glu Thr Ile Thr Glu Lys Leu 
    370                 375                 380                 


Arg Glu Ile Gly Leu Thr Leu Ala Val Leu Asp Val Leu Leu Thr Ala 
385                 390                 395                 400 


Leu Asp Ala Gly Val Phe Ala Lys Phe Lys Gly Ala Ala His Ile Ser 
                405                 410                 415     


Thr Lys Ala Ala Arg Asn Leu Leu Pro His Leu Glu Gln Gly Arg Arg 
            420                 425                 430         


Tyr Asp Glu Ala Cys Thr Met Ala Gly Tyr Asp His Ala Ala Ser Arg 
        435                 440                 445             


Leu Ser His His Gly Gln Ile Val Ala Lys Thr Gln Phe Asn Ala Leu 
    450                 455                 460                 


Val Thr Glu Ile Gly Glu Ser Ile Ala Asn Pro Ile Ala Arg Lys Ala 
465                 470                 475                 480 


Leu Ile Glu Gly Leu Lys Gln Ile Trp Ala Met Arg Asn His Trp Gly 
                485                 490                 495     


Leu Pro Gly Ser Ile His Val Glu Leu Ala Arg Asp Val Gly Asn Ser 
            500                 505                 510         


Ile Glu Lys Arg Arg Glu Ile Glu Lys His Ile Glu Lys Asn Thr Ala 
        515                 520                 525             


Leu Arg Ala Arg Glu Arg Arg Glu Val His Asp Leu Leu Asp Leu Glu 
    530                 535                 540                 


Asp Val Asn Gly Asp Thr Leu Leu Arg Tyr Arg Leu Trp Lys Glu Gln 
545                 550                 555                 560 


Gly Gly Lys Cys Leu Tyr Thr Gly Lys Ala Ile His Ile Arg Gln Ile 
                565                 570                 575     


Ala Ala Thr Asp Asn Ser Val Gln Val Asp His Ile Leu Pro Trp Ser 
            580                 585                 590         


Arg Phe Gly Asp Asp Ser Phe Asn Asn Lys Thr Leu Cys Leu Ala Ser 
        595                 600                 605             


Ala Asn Gln Gln Lys Lys Arg Ser Thr Pro Tyr Glu Trp Leu Ser Gly 
    610                 615                 620                 


Gln Thr Gly Asp Ala Trp Asn Ala Phe Val Gln Arg Ile Glu Thr Asn 
625                 630                 635                 640 


Lys Glu Leu Arg Gly Phe Lys Lys Arg Asn Tyr Leu Leu Lys Asn Ala 
                645                 650                 655     


Lys Glu Ala Glu Glu Lys Phe Arg Ser Arg Asn Leu Asn Asp Thr Arg 
            660                 665                 670         


Tyr Ala Ala Arg Leu Phe Ala Glu Ala Val Lys Leu Leu Tyr Ala Phe 
        675                 680                 685             


Gly Glu Arg Gln Glu Lys Gly Gly Asn Arg Arg Val Phe Thr Arg Pro 
    690                 695                 700                 


Gly Ala Leu Thr Ala Ala Leu Arg Gln Ala Trp Gly Val Glu Ser Leu 
705                 710                 715                 720 


Lys Lys Gln Asp Gly Lys Arg Ile Asn Asp Asp Arg His His Ala Leu 
                725                 730                 735     


Asp Ala Leu Thr Val Ala Ala Val Asp Glu Ala Glu Ile Gln Arg Leu 
            740                 745                 750         


Thr Lys Ser Phe His Glu Trp Glu Gln Gln Gly Leu Gly Arg Pro Leu 
        755                 760                 765             


Arg Arg Val Glu Pro Pro Trp Glu Ser Phe Arg Ala Asp Val Glu Ala 
    770                 775                 780                 


Thr Tyr Pro Glu Val Phe Val Ala Arg Pro Glu Arg Arg Arg Ala Arg 
785                 790                 795                 800 


Gly Glu Gly His Ala Ala Thr Ile Arg Gln Val Lys Glu Arg Glu Cys 
                805                 810                 815     


Thr Pro Ile Val Phe Glu Arg Lys Ala Val Ser Ser Leu Lys Glu Ala 
            820                 825                 830         


Asp Leu Glu Arg Ile Lys Asp Gly Glu Arg Asn Glu Ala Ile Val Glu 
        835                 840                 845             


Ala Ile Arg Ser Trp Ile Ala Thr Gly Arg Pro Ala Asp Ala Pro Pro 
    850                 855                 860                 


Arg Ser Pro Arg Gly Asp Ile Ile Thr Lys Ile Arg Leu Ala Thr Thr 
865                 870                 875                 880 


Ile Lys Ala Ala Val Pro Val Arg Gly Gly Thr Ala Gly Arg Gly Glu 
                885                 890                 895     


Met Val Arg Ala Asp Val Phe Ser Lys Pro Asn Arg Arg Gly Lys Asp 
            900                 905                 910         


Glu Trp Tyr Leu Val Pro Val Tyr Pro His Gln Ile Met Asn Arg Lys 
        915                 920                 925             


Ala Trp Pro Lys Pro Pro Met Arg Ser Ile Val Ala Asn Lys Asp Glu 
    930                 935                 940                 


Asp Glu Trp Thr Glu Val Gly Pro Glu His Gln Phe Arg Phe Ser Leu 
945                 950                 955                 960 


Tyr Pro Arg Ser Asn Ile Glu Ile Ile Arg Pro Ser Gly Glu Val Ile 
                965                 970                 975     


Glu Gly Tyr Phe Val Gly Leu His Arg Asn Thr Gly Ala Leu Ile Pro 
            980                 985                 990         


Thr Pro Val Gly Pro Asp Ser Tyr  Val Ile Ala 
        995                 1000             


<210>  476
<211>  1007
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  476

Met Lys Val Leu Gly Phe Asp Ile Gly Ile Asn Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Asn Asn Glu Leu Lys Asp Cys Gly Val Arg Leu Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Thr Lys Glu Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Asn Ala Arg Ser Ser Arg Val Arg Leu Arg Arg Arg Arg Ser Arg Leu 
    50                  55                  60                  


Leu Ser Leu Lys His Ile Ile Ser Lys Glu Phe Gly Leu His Tyr Glu 
65                  70                  75                  80  


Asp Tyr Ile Ala Leu Asp Gly Asn Leu Pro Lys Ala Tyr Thr Gly Lys 
                85                  90                  95      


Ile Glu Ser Ile Tyr Lys Leu Arg Tyr Glu Ala Leu Ser Gln Ala Ile 
            100                 105                 110         


Ser Lys Glu Asp Leu Ala Arg Val Ile Leu His Ile Ala Lys His Arg 
        115                 120                 125             


Gly Tyr Met Asn Lys Asn Gln Lys Thr Ser Gln Asp Ser Glu Lys Gly 
    130                 135                 140                 


Ala Ile Leu Ser Ala Ile Lys Glu Asn Ser Asn Lys Ile Lys His Tyr 
145                 150                 155                 160 


Gln Thr Ala Gly Glu Tyr Phe Tyr Lys Glu Leu Phe Glu Asn Leu Arg 
                165                 170                 175     


Asp Gly Thr Lys Glu Phe Lys Asn Ile Arg Asn Lys Ala Met Lys Asn 
            180                 185                 190         


Glu Lys Thr Gly Asn Met Glu Ser Thr Tyr Tyr Asn Thr Ile Leu Ala 
        195                 200                 205             


Ser Asp Leu Glu Lys Glu Leu Lys Leu Ile Leu Glu Lys Gln Gln Ala 
    210                 215                 220                 


Tyr His Asn Tyr Ser Asp Thr Phe Ile Lys Glu Val Leu Glu Ile Ala 
225                 230                 235                 240 


Phe Tyr Gln Arg Pro Leu Lys Asp Phe Ser His Leu Val Gly Ser Cys 
                245                 250                 255     


Thr Phe Tyr Glu Asn Glu Lys Arg Ala Cys Lys Asn Ser Tyr Ser Ala 
            260                 265                 270         


Trp Glu Phe Ile Ala Leu Gly Lys Ile Ile Asn Thr Leu Glu Ser Ile 
        275                 280                 285             


Lys Lys Asp Thr Gly Glu Ile Phe Ser Asn Glu Ile Ile Thr Gln Ile 
    290                 295                 300                 


Leu Lys Glu Val Leu Asp Lys Gly Ser Leu Ser Tyr Thr Lys Leu Arg 
305                 310                 315                 320 


Lys Met Ile Asn Leu Asp Glu Lys Val Lys Phe Lys Gly Leu Lys Tyr 
                325                 330                 335     


Asp Lys Glu Gly Val Glu Lys Thr Asn Leu Ile Glu Phe Lys Lys Leu 
            340                 345                 350         


Lys Glu Phe Lys Lys Ala Leu Gly Glu His Asn Leu Glu Arg Lys Thr 
        355                 360                 365             


Leu Asp Glu Ile Ala Thr His Ile Thr Leu Thr Lys Asp Glu Asn Gln 
    370                 375                 380                 


Leu Lys Lys Gln Leu Glu Gln Tyr Ala Leu Ser Gln Asn Gln Ile Asp 
385                 390                 395                 400 


Ala Leu Ala Glu Leu Asp Phe Asn Asp His Ile Asn Leu Ser Phe Lys 
                405                 410                 415     


Ala Leu Tyr Glu Ile Leu Pro Leu Met Glu Lys Gly Lys Arg Tyr Asp 
            420                 425                 430         


Glu Ala Cys Asn Glu Leu Gly Leu Lys Ala Lys Thr Ser Asn Gln Lys 
        435                 440                 445             


Ser Glu Phe Leu Pro Ala Phe Cys Asp Ser Ile Phe Ala Gln Glu Leu 
    450                 455                 460                 


Thr Asn Pro Ile Val Asn Arg Ala Ile Ser Glu Tyr Arg Lys Val Leu 
465                 470                 475                 480 


Asn Arg Leu Leu Lys Lys Tyr Gly Lys Met His Lys Ile His Ile Glu 
                485                 490                 495     


Leu Ala Arg Asp Ala Gly Leu Ser Lys Glu Ala Arg Asn Lys Ile Glu 
            500                 505                 510         


Lys Glu Gln Lys Glu Asn Lys Asp Ile Asn Asp Lys Ala Glu Lys Thr 
        515                 520                 525             


Cys Lys Glu Phe Gly Leu Lys Pro Asn Ala Lys Asn Ile Leu Lys Ile 
    530                 535                 540                 


Lys Leu Trp Arg Glu Gln Gly Glu Ile Cys Ala Tyr Ser Gly Lys Lys 
545                 550                 555                 560 


Ile Thr Ile Asp Asp Leu Lys Glu Asp Lys Ala Leu Glu Val Asp His 
                565                 570                 575     


Ile Tyr Pro Tyr Ser Arg Ser Tyr Asp Asp Ser Tyr Asn Asn Lys Val 
            580                 585                 590         


Leu Val Phe Ile Lys Glu Asn Gln Leu Lys Leu Asn Gln Thr Pro Tyr 
        595                 600                 605             


Glu Ala Phe Gly Ser Asn Gln Glu Lys Trp Ser Lys Ile Gln Val Leu 
    610                 615                 620                 


Ala Gln Lys Leu Pro Tyr Lys Lys Lys Asn Arg Ile Leu Asp Glu Asn 
625                 630                 635                 640 


Phe Lys Asn Lys Asp Gln Ile Glu Phe Ile Ser Arg Asn Leu Asn Asp 
                645                 650                 655     


Thr Arg Tyr Ala Thr Ser Leu Ile Ala Lys Tyr Thr Lys Glu Tyr Leu 
            660                 665                 670         


Glu Phe Leu Pro Leu Ser Asp Asp Glu Asp Ile Ser Leu Lys Ser Gly 
        675                 680                 685             


Glu Lys Gly Ser Lys Thr His Val Gln Thr Met Asn Gly Met Leu Thr 
    690                 695                 700                 


Ser Val Leu Arg His Ala Trp Gly Phe Ser Gln Lys Asp Arg Asn Asn 
705                 710                 715                 720 


His Leu His His Ala Leu Asp Ala Ile Ile Ile Ala Tyr Ser Thr Asn 
                725                 730                 735     


Ser Ile Ile Lys Ala Phe Ser Asp Phe Lys Lys Asn Gln Glu Ile Leu 
            740                 745                 750         


Lys Ala Lys Leu Tyr Ala Lys Lys Leu Thr Ser Glu Glu Tyr Lys Asn 
        755                 760                 765             


Gln Ser Lys Phe Thr Gly Phe Glu Lys Tyr Arg Ser Lys Ile Leu Glu 
    770                 775                 780                 


Lys Leu Asp Ser Ile Phe Val Ser Lys Pro Pro Asn Lys Asn Thr Lys 
785                 790                 795                 800 


Gly Ala Leu His Glu Gln Thr Phe Tyr Ser Tyr Gln Asp Ile Leu Glu 
                805                 810                 815     


Glu Tyr Lys Thr Gln Glu Gly Leu Lys Arg Ala Leu Glu Cys Gly Lys 
            820                 825                 830         


Val Arg Lys Ile Gly Thr Lys Tyr Val Val Asn Asp Arg Met Val Arg 
        835                 840                 845             


Leu Asp Ile Phe Lys Lys Asp Asn Lys Phe Tyr Gly Ile Pro Ile Tyr 
    850                 855                 860                 


Thr Met Asp Phe Ala Leu Gly Val Leu Pro Asn Lys Val Val Thr Ile 
865                 870                 875                 880 


Gly Thr Asp Lys Asp Lys Ile Lys Lys Glu Trp Lys Thr Ile Asp Glu 
                885                 890                 895     


Thr Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Gly Asp Leu Val Leu Ile 
            900                 905                 910         


Gln Lys Lys Glu Met Gln Glu Ser Glu Phe Ala Tyr Tyr Asn Gly Phe 
        915                 920                 925             


Asp Ile Ser Ser Ala Ser Ile Ser Leu Glu Lys His Asp Asn Lys Phe 
    930                 935                 940                 


Glu Asn Leu Thr Glu Asn Gln Lys Leu Leu Phe Pro Lys Ala Lys Glu 
945                 950                 955                 960 


Gly Asp Val Glu Val Arg Gly Ile Gly Ile Gln Gly Leu Lys Val Phe 
                965                 970                 975     


Lys Lys Tyr Met Val Thr Pro Leu Gly Glu Ile Gln Glu Thr Arg Thr 
            980                 985                 990         


Glu Ala Arg Gln Asn Ile Gln Pro  Arg Lys Lys His Gly  Leu Arg 
        995                 1000                 1005         


<210>  477
<211>  1010
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  477

Met Ser Asp Ser Ser Tyr Val Leu Gly Leu Asp Leu Gly Thr Asn Ser 
1               5                   10                  15      


Ile Gly Trp Ala Cys Val Arg Leu Asn Lys Gly Lys Pro Asp Gly Leu 
            20                  25                  30          


Leu Ala Gln Gly Val Arg Ile Phe Glu Ala Gly Leu Glu Ile Asp Ala 
        35                  40                  45              


Tyr Gly Arg Gly Thr Pro Leu Asn Thr Glu Arg Arg Asn Ala Arg Gln 
    50                  55                  60                  


Arg Arg Arg Leu His Asp Arg Arg Lys Arg Arg Ile Leu Lys Val Arg 
65                  70                  75                  80  


Arg Ile Leu Gln Glu Ala Gly Met Leu Pro Ala Asn Lys Pro Asp His 
                85                  90                  95      


Lys Asp Ala Glu Trp Lys Met Leu Leu Ala Arg Asn Pro Tyr Arg Leu 
            100                 105                 110         


Arg Ala Lys Ala Leu Asp Glu Gln Ile Asp Leu Lys Glu Ile Gly Leu 
        115                 120                 125             


Cys Ile Tyr Gln Phe Ala His Arg Arg Gly Phe Leu Ser Asn Ser Lys 
    130                 135                 140                 


Ile Leu Lys Lys Asp Asp Asp Arg Gly Lys Leu Lys Asp Ala Thr Lys 
145                 150                 155                 160 


Ala Phe Ala Glu Asp Met Glu Gln Lys Gly Ala Arg Thr Trp Gly Glu 
                165                 170                 175     


Tyr Leu Ser His Leu Asp Pro His Glu Gln Arg Ile Arg Thr Asn Tyr 
            180                 185                 190         


Thr Leu Arg Glu Trp Tyr Thr Ser Glu Phe Asp Leu Ile Trp Glu Lys 
        195                 200                 205             


Gln Gln Ser Phe Tyr Pro His Val Leu Thr Asp Asp Leu Tyr Lys Arg 
    210                 215                 220                 


Val Lys Glu Ala Leu Phe Tyr Gln Arg Pro Leu Lys Ser Gln Ala His 
225                 230                 235                 240 


Leu Leu Gly Gln Cys Glu Leu Glu Pro Gly Arg Lys Arg Ala Pro Phe 
                245                 250                 255     


Ala Leu Leu Met Ala Gln Glu Phe Arg Leu Leu Gln Ser Leu Asn His 
            260                 265                 270         


Leu Arg Val Val Ser Ser Thr Gly Asp Glu Arg Leu Leu Thr Asp Glu 
        275                 280                 285             


Glu Arg Lys Gln Ala Leu Ala Leu Leu Arg Ser Arg Gly Asn Gln Lys 
    290                 295                 300                 


Phe Thr Thr Leu Arg Lys Val Leu Asn Leu Arg Gly Thr Lys Phe Asn 
305                 310                 315                 320 


Leu Glu Arg Gly Gly Glu Lys Thr Ile Gln Gly Asn Asn Thr Ala Ala 
                325                 330                 335     


Ala Leu Gly Asn Leu Phe Gly Glu Ala Trp Asp His Phe Ser Ser Glu 
            340                 345                 350         


Gln Lys Asn Gln Ile Val Glu Asp Arg Leu Thr Ile Gly Asp Thr Asp 
        355                 360                 365             


Ala Leu Ile Arg Arg Met Lys Lys Thr Trp Gly Leu Asp Ser Val Thr 
    370                 375                 380                 


Ala Glu Met Phe Ser Glu Thr Glu Leu Glu Asp Gly Tyr Cys Gly Phe 
385                 390                 395                 400 


Ser Arg Lys Ala Leu Glu Lys Leu Leu Pro Leu Leu Glu Arg Gly His 
                405                 410                 415     


Ala Leu Met Glu Ala Val Gln Ala Ala Tyr Pro Asp Tyr Gln Lys Asn 
            420                 425                 430         


Leu Pro Arg Thr Asp Thr Leu Pro Pro Leu Glu Asn Leu Arg Asn Pro 
        435                 440                 445             


Ile Val Gln Arg Thr Leu Ser Glu Leu Arg Arg Val Val Asn Ala Ile 
    450                 455                 460                 


Ile Lys Glu Tyr Gly Lys Ser Asp Lys Ile Arg Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Asp Met Lys Gln Thr Ala Lys Asn Arg Glu Lys Ser Trp Asp Arg Met 
                485                 490                 495     


Arg Val Arg Glu Arg Gln Arg Lys Glu Ala Ala Lys Lys Ile Leu Glu 
            500                 505                 510         


Glu Thr Gly Asn Pro Ala Pro Arg Arg His Glu Ile Glu Lys Val Leu 
        515                 520                 525             


Leu Ala Glu Glu Cys Asn Trp Glu Cys Pro Tyr Thr Gly Lys Arg Ile 
    530                 535                 540                 


Cys Met Asn Thr Leu Leu Gly Asp Ala Pro Gln Phe Asp Ile Glu His 
545                 550                 555                 560 


Ile Ile Pro Phe Ser Arg Ser Phe Asp Asn Ser Phe Ser Asn Lys Thr 
                565                 570                 575     


Leu Cys Asp Val Asn Tyr Asn Arg Asn Val Lys Gly Asn Lys Thr Pro 
            580                 585                 590         


Tyr Glu Ser Ala Ser Glu Thr Glu His Glu Glu Met Val Cys Arg Val 
        595                 600                 605             


Lys Arg Phe Thr Gly Asp Tyr Lys Glu Ile Lys Leu Asp Arg Phe Asn 
    610                 615                 620                 


Thr Arg Glu Thr Asp Ser Arg Ala Asp Phe Ile Glu Glu Phe Ser Thr 
625                 630                 635                 640 


Ser Gln Leu Asn Asp Thr Arg Tyr Ala Ser Arg Leu Ala Gly Glu Tyr 
                645                 650                 655     


Leu Gly Lys Leu Tyr Gly Ser Glu Trp Arg Lys His Ile Gln Ile Ala 
            660                 665                 670         


Lys Gly Gly Thr Thr Lys Tyr Leu Arg Asp Ala Trp Asp Leu Asn Arg 
        675                 680                 685             


Ile Leu Ser Asp Gly Gly Thr Lys Ser Arg Asp Asp His Arg His His 
    690                 695                 700                 


Ala Val Asp Ala Val Val Val Ala Leu Thr Thr Pro Gln Thr Val Lys 
705                 710                 715                 720 


Ala Leu Ser Asp Ala Asn Gln Arg Ala Pro Glu His Arg Arg Tyr Ala 
                725                 730                 735     


Phe Ser Asp Met Pro Leu Pro Trp Glu Thr Phe Arg Gln Asp Val Glu 
            740                 745                 750         


Gln Ala Ile Asn Ser Ile Thr Val Ser His Lys Val Cys Arg Lys Ala 
        755                 760                 765             


Asn Gly Gln Leu His Lys Glu Thr Phe Tyr Gly Leu Ile Lys Asp Arg 
    770                 775                 780                 


Lys Thr Gly Asp Thr Arg Thr Val Leu Arg Lys Pro Leu His Glu Leu 
785                 790                 795                 800 


Thr Asp Lys Glu Ile Lys Arg Ala Glu Ile Val Asp Ser Ser Ile Arg 
                805                 810                 815     


Lys Leu Ile Glu Gln Lys Leu Lys Asp Leu Asp Gln Pro Pro Lys Thr 
            820                 825                 830         


Ala Phe Lys Asp Arg Asn Asn His Pro Phe Ile Ile Asp Lys Asn Gly 
        835                 840                 845             


Asn Lys Arg Phe Ile His Lys Val Arg Val Tyr Gln Asp Ala Lys Pro 
    850                 855                 860                 


Val Lys Ile Ala Glu Asn Arg His Val Lys Leu Gly Gly Asn His His 
865                 870                 875                 880 


Leu Glu Ile Phe Glu Val Ile Asp Lys Lys Gly Asn Pro Thr Trp Lys 
                885                 890                 895     


Cys Glu Val Ile Ser Leu Phe Asp Thr Arg Gln Arg Val Lys Glu Lys 
            900                 905                 910         


Arg Pro Val Ile Val Arg Gln Asp Gln Asn Gly Asn Pro Leu Lys Phe 
        915                 920                 925             


Ser Leu Ser Ile Gly Glu Ala Val Gln Leu Asp Trp Asn Gly Gly Arg 
    930                 935                 940                 


Glu Thr Ala Val Val Gln Lys Met Ser Lys Thr Pro Asn Thr Val Glu 
945                 950                 955                 960 


Tyr Val Phe Arg Lys His Asn Asp Ala Arg Lys Ala Ala Asp Ile Pro 
                965                 970                 975     


Tyr Lys Glu Thr Ile Thr Val Lys Ser Asp Ser Ala Leu Phe Ala Ser 
            980                 985                 990         


Asn Leu Gln Lys Ile Val Ile Thr  Pro Thr Gly Leu Ile  Arg Arg Ala 
        995                 1000                 1005             


Asn Asp  
    1010 


<210>  478
<211>  1010
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  478

Met Lys Asn Arg Asn Val Ile Gly Ile Asp Val Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Trp Ala Leu Val Glu Glu Asn Glu Asn Gly Leu Pro Gln Ser Leu 
            20                  25                  30          


Lys Ala Ala Gly Val Arg Ile Phe Gln Glu Ala Val Glu Ala Lys Thr 
        35                  40                  45              


Arg Thr Pro Lys Asn His Lys Arg Arg Gln Ala Arg Leu Ala Arg Arg 
    50                  55                  60                  


Val Leu Ala Arg Arg Ala Arg Arg Lys Gln Arg Leu Gln Asn Leu Leu 
65                  70                  75                  80  


Leu Lys His Gly Leu Leu Pro Gly Gly Val Thr Asp Pro Val Gln Arg 
                85                  90                  95      


Glu Ala Ala Phe Thr Thr Leu Gly Asp Pro Tyr Gln Leu Arg Ala Lys 
            100                 105                 110         


Gly Leu Asn Leu Pro Leu Gly Leu His Glu Phe Gly Arg Val Leu Phe 
        115                 120                 125             


Gln Leu Gly Ala Arg Arg Gly Phe Leu Ser Asn Arg Lys Thr Arg Tyr 
    130                 135                 140                 


Gly His Leu Asn Lys Leu Asp Glu Leu Ala Asp Leu Ile Glu Ala Glu 
145                 150                 155                 160 


Asp Ala Ala Pro Lys Thr Gly Asp Ala Glu Glu Gly Ile Ile Arg Lys 
                165                 170                 175     


Glu Ile Gly Glu Leu Arg Gln Ala Met Ser Ala Ala Gly Ser Arg Thr 
            180                 185                 190         


Leu Gly Glu Tyr Leu Ala Gly Leu Ala Lys Glu Glu Arg Lys Arg Cys 
        195                 200                 205             


Arg His Thr Glu Arg Ala Met Leu Glu Glu Glu Phe Glu Arg Leu Trp 
    210                 215                 220                 


Met Val Gln Ala Arg Phe His Pro Glu Leu Ser Asp Glu Leu Lys Val 
225                 230                 235                 240 


Gln Val His Thr Val Ile Phe Phe Gln Arg Pro Leu Lys Ser Asn Arg 
                245                 250                 255     


Ala Ser Leu Gly Lys Cys Ser Leu Glu Pro Ser Arg Arg Lys Ala Ala 
            260                 265                 270         


Arg Ala Trp Pro Glu Ala Gln Arg Phe Arg Leu Leu Gln Asp Leu Asn 
        275                 280                 285             


Asn Leu Glu Ile Leu Asp Pro Arg Thr Gly Glu Leu Arg Arg Leu Arg 
    290                 295                 300                 


Asp Thr Glu Arg Ala Ile Leu Leu Asn Ala Leu Glu Gln Gln Lys Ser 
305                 310                 315                 320 


Met Thr Trp Asn Ala Val Arg Lys Lys Leu Gly Leu His Gln Gly Glu 
                325                 330                 335     


Lys Phe Asn Leu Glu Thr Gly Ala Lys Ala Glu Thr Gly Leu Lys Gly 
            340                 345                 350         


Leu Gln Thr Leu Ala Ser Leu His Ala Ile Leu Gln Gly Trp Phe Asp 
        355                 360                 365             


Glu Leu Gly Asp Lys Arg Asp Leu Leu Ile Glu Asp Leu Ile Thr Ile 
    370                 375                 380                 


Glu Asp Ser Ala Gly Leu Tyr Arg Arg Leu Arg Glu His Trp Gly Phe 
385                 390                 395                 400 


Asp Arg Glu Thr Ala Phe Arg Leu Ala Thr Leu Glu Leu Glu Pro Gly 
                405                 410                 415     


Tyr Ala Ser Leu Ser Leu Lys Ala Ile Arg Lys Leu Leu Pro His Met 
            420                 425                 430         


Gly Ala Gly His Arg Tyr Ser Glu Ala Arg Gln Gln Val Gly Tyr Gly 
        435                 440                 445             


Tyr Glu Thr Gly Gln Arg Asp Leu Gln Glu Arg Leu Gly Glu Pro Pro 
    450                 455                 460                 


Asp Pro Arg Asn Pro Val Val Asp Lys Ala Leu His Glu Leu Arg Arg 
465                 470                 475                 480 


Val Ile Asn Ala Leu Ile His Arg Phe Gly Arg Pro Asp Val Val Arg 
                485                 490                 495     


Val Glu Leu Ala Arg Asp Leu Lys Leu Ala Lys Arg Asp Lys Glu Arg 
            500                 505                 510         


Leu Glu Lys Gln Asn Arg Glu Asn Gln Arg Leu Asn Arg Lys Ala Glu 
        515                 520                 525             


Ala Ala Leu Ala Glu Leu Gly Leu Gly Gln Pro Ser Arg Asp Asp Leu 
    530                 535                 540                 


Ile Lys Tyr Arg Leu Trp Glu Glu Ser Lys Gly Leu Cys Pro Tyr Thr 
545                 550                 555                 560 


Gly Arg Glu Ile Gly Leu Ser Thr Leu Phe Ser Pro Glu Val Asp Val 
                565                 570                 575     


Glu His Ile Ile Pro Tyr Ser Arg Cys Leu Asp Asp Ser Tyr Met Asn 
            580                 585                 590         


Lys Thr Leu Cys Met Thr Glu Glu Asn Arg Met Gln Lys Gly Asn Arg 
        595                 600                 605             


Thr Pro Trp Glu Ala Tyr Gly Gly Asp Pro Ala Arg Tyr Glu Gln Ile 
    610                 615                 620                 


Leu Gln Cys Ala Arg Tyr Phe Pro Met Ala Lys Arg Lys Arg Phe Leu 
625                 630                 635                 640 


Glu Gln Gly Ser Asp Phe Val Asp Gln Phe Ile Gln Arg Gln Leu Asn 
                645                 650                 655     


Asp Thr Arg Tyr Ile Ala Lys Leu Ala Lys Glu Tyr Leu Ala Ala Val 
            660                 665                 670         


Cys Thr Asn Val Gln Val Thr Lys Gly Gly Thr Thr Ala Met Leu Arg 
        675                 680                 685             


His His Trp Gly Leu Asn Arg Leu Leu Gly Glu Asp Asp Gly Gln Lys 
    690                 695                 700                 


Glu Arg Gly Asp His Arg His His Ala Leu Asp Ala Ala Val Ile Ala 
705                 710                 715                 720 


Val Thr Ser Arg Ser Leu Tyr Gln Arg Leu Ala Arg Ile Ala His Gln 
                725                 730                 735     


Arg Gly His Ala Pro Ser Gln Arg Gly Phe Tyr Ile Asp Pro Pro Trp 
            740                 745                 750         


Gln Gly Tyr Gln Ala Asp Leu Ala Gln Thr Leu Ala Gly Ile Val Val 
        755                 760                 765             


Ser His Ala Thr Asn Arg Lys Leu Thr Gly Ala Leu His Glu Glu Thr 
    770                 775                 780                 


Ala Tyr Gly His Ile Pro His Val Gly Phe Val Tyr Arg Lys Thr Leu 
785                 790                 795                 800 


Asn Lys Asp Phe Lys Leu Gly Asp Ile Glu Ala Ile Tyr Asp Pro Ser 
                805                 810                 815     


Leu Gln Glu Leu Val Lys Ala His Leu Glu His His Gly Asn Asp Pro 
            820                 825                 830         


Lys Arg Ala Phe Ala Glu Gly Ile Gln Cys Tyr His Lys Asp Gly His 
        835                 840                 845             


Thr Pro Ile Lys Arg Val Arg Ile Arg Ala Lys Gln Val Ser Glu Lys 
    850                 855                 860                 


Gly Thr Phe Ser Ile Ser Asp Arg Ala Gly Arg Ala Tyr Lys His His 
865                 870                 875                 880 


Lys Thr Gly Ser Asn His His Val Glu Ile Leu Arg His Arg Ala Thr 
                885                 890                 895     


Gly Lys Ile Glu Pro Val Tyr Leu Thr Thr Lys Glu Ala Thr Glu Arg 
            900                 905                 910         


Ala Arg Arg Gly Gly Glu Pro Val Ile Gln Arg Asp His Gly Pro Glu 
        915                 920                 925             


Trp Glu Phe Ile Ile Ser Leu Cys Ile Asn Asp Met Val Arg Leu Gly 
    930                 935                 940                 


Glu Gly Gly Gln Val Ala Tyr Phe Arg Val Gln Lys Ile Glu Ala Thr 
945                 950                 955                 960 


Asn Asn Arg Val Thr Leu Arg Ser His Leu Ala Ala Thr Leu Asp Asp 
                965                 970                 975     


Glu Asp Met Lys Val Thr Thr Ser Val Pro Lys Leu Ala Val Met Pro 
            980                 985                 990         


Gly Phe Gln Lys Val Leu Val Thr  Ala Val Gly Glu Val  Leu Glu Leu 
        995                 1000                 1005             


His Asp  
    1010 


<210>  479
<211>  1010
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  479

Met Lys Arg Met Ala Ala Val Gly Leu Met Pro Ala Asp Pro Thr Glu 
1               5                   10                  15      


Ala Lys Ala Leu Glu Leu Leu Asp Pro Tyr Ala Leu Arg Ala Ala Gly 
            20                  25                  30          


Leu Asp Gln Ala Leu Pro Leu Thr His Phe Gly Arg Ala Leu Phe His 
        35                  40                  45              


Leu Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Gly 
    50                  55                  60                  


Asp Asn Glu Ser Gly Lys Ile Lys Asp Ala Thr Lys Arg Leu Asp Glu 
65                  70                  75                  80  


Glu Met Ala Ile Lys Asn Ala Arg Thr Tyr Gly Glu Phe Leu His Met 
                85                  90                  95      


Arg Arg Thr Ser Ala Thr Asp Pro Lys Asn Val Pro Thr Val Arg Thr 
            100                 105                 110         


Arg Leu Ser Ile Ala Arg Arg Asp Asn Ala Glu Lys Glu Glu Ala Gly 
        115                 120                 125             


Tyr Asp Phe Tyr Pro Asp Arg Arg His Leu Ala Glu Glu Phe Asp Lys 
    130                 135                 140                 


Leu Trp Lys Ala Gln Ala Gly Tyr Ser Pro Asp Val Leu Thr Asp Glu 
145                 150                 155                 160 


Leu Arg Asp Glu Ile Ser Val Ile Ile Phe His Gln Arg Pro Leu Lys 
                165                 170                 175     


Thr Pro Glu Val Gly Leu Cys Leu Phe Ser Gly Ser His Gly Val Pro 
            180                 185                 190         


Lys Lys Asp Lys Arg Ile Pro Ser Ala His Pro Leu Asn Gln Arg Arg 
        195                 200                 205             


Ile Leu Phe Glu Thr Ile Asn Asn Leu Arg Val Ala Ala Arg Gly Glu 
    210                 215                 220                 


Pro Ala Arg Gly Leu Thr Arg Asp Glu Arg Asp Ala Ile Val His Ala 
225                 230                 235                 240 


Leu Asp Asn Lys Ala His Thr Lys Ser Leu Ser Gly Met Ser Met Lys 
                245                 250                 255     


Leu Lys Ala Leu Gly Lys Val Ile Lys Leu Arg Pro Glu Gln Ser Phe 
            260                 265                 270         


Thr Leu Gln Thr Ala Asn Arg Glu Thr Ile Ala Cys Asp Pro Val Arg 
        275                 280                 285             


Ala Ser Leu Ser His Pro Asp Arg Phe Gly Pro Arg Trp Ser Met Leu 
    290                 295                 300                 


Asp Ala Asp Ala Gln Trp Asp Val Val Gln Arg Ile Arg Ala Val Gln 
305                 310                 315                 320 


Ser Glu Ala Glu His Asp Ala Leu Val Lys Trp Leu Met Ala Thr His 
                325                 330                 335     


Gly Leu Asp Arg Asp His Ala Glu Ser Thr Ala Lys Ala Pro Leu Pro 
            340                 345                 350         


Glu Gly Tyr Gly Arg Leu Gly Leu Thr Ala Thr Lys Arg Ile Leu Ala 
        355                 360                 365             


Ala Leu Glu Ala Asp Val Ile Pro Tyr Ser Asp Ala Val Lys Ala Cys 
    370                 375                 380                 


Gly Trp His His Ser Asp Gly Arg Thr Gly Glu Val Leu Thr Glu Leu 
385                 390                 395                 400 


Pro Tyr Tyr Gly Gln Ile Leu Asp Arg His Val Ile Pro Gly Thr Tyr 
                405                 410                 415     


Asn Glu Ser Asp Asp Asp Val Thr Arg Tyr Gly Arg Ile Thr Asn Pro 
            420                 425                 430         


Thr Val His Ile Gly Leu Asn Gln Leu Arg Arg Leu Val Asn Lys Ile 
        435                 440                 445             


Ile Thr Val Tyr Gly Lys Pro Asp Glu Ile Val Val Glu Leu Ala Arg 
    450                 455                 460                 


Asp Leu Lys Leu Ser Glu Asp Gln Lys Arg Asp Val Gln Arg Asp Ile 
465                 470                 475                 480 


Lys Lys Asn Thr Asp Ala Ala Ile Ala Arg Gly Ala Lys Leu Ser Glu 
                485                 490                 495     


Met Gly Val Pro Asp Thr Gly Ala Asn Arg Met Val Leu Arg Leu Trp 
            500                 505                 510         


Glu Ser Leu Gly Asp Asp Val Met Thr Arg Asn Cys Pro Tyr Ser Gly 
        515                 520                 525             


Lys Arg Ile Ser Ala Thr Met Leu Phe Asp Gly Ser Cys Asp Val Asp 
    530                 535                 540                 


His Ile Leu Pro Tyr Ser Arg Thr Leu Asp Asp Gly Phe Ala Asn Arg 
545                 550                 555                 560 


Thr Leu Cys Leu Lys Glu Phe Asn Arg Gln Lys Thr Asp Lys Thr Pro 
                565                 570                 575     


Trp Glu Ala Trp Gly Asp Thr Pro Ala Trp Ala Thr Ile Glu Ala Asn 
            580                 585                 590         


Leu Lys Asn Leu Pro Ala Asn Lys Ala Trp Arg Phe Ala Pro Asp Ala 
        595                 600                 605             


Met Glu Arg Phe Glu Gly Glu Asn Asp Phe Thr Ser Arg Ala Leu Lys 
    610                 615                 620                 


Asp Ala Gln Tyr Leu Ser Arg Ile Ala Arg Thr Tyr Leu Asp Ala Leu 
625                 630                 635                 640 


Tyr Asp Gly Ala Asp Gly Lys Ser His Val Trp Val Val Pro Gly Arg 
                645                 650                 655     


Leu Thr Glu Met Leu Arg Arg His Trp Gly Leu Asn Ser Leu Leu Pro 
            660                 665                 670         


Asp Lys Asp Gly Ala Val Lys Ala Lys Asn Arg Thr Asp His Arg His 
        675                 680                 685             


His Ala Ile Asp Ala Ala Val Val Ala Ala Thr Asp Arg Ser Leu Ile 
    690                 695                 700                 


Gln Arg Ile Ser Lys Met Ala Arg Lys Asp Glu Val Asn Gly Ala Glu 
705                 710                 715                 720 


Glu Val Ala Arg Ser Val Pro Pro Pro Trp Glu Gly Phe Arg Thr Asp 
                725                 730                 735     


Ile Glu Thr Gln Leu Asp Arg Ile Ile Ile Ser His Arg Ala Asp His 
            740                 745                 750         


Gly Arg Ile Asp Pro Ala Ala Arg Lys Asp Gly His Asp Ser Thr Ser 
        755                 760                 765             


Gly Gln Leu His Asn Asp Thr Ala Tyr Gly Leu Gly Glu Gly Asn Ser 
    770                 775                 780                 


Val Val Ser Arg Lys Pro Leu Leu Ser Leu Lys Ser Asn Asp Ile Glu 
785                 790                 795                 800 


Val Thr Thr Lys Gly Ala Asn Ile Arg Asp Val Asp Leu Gln Arg His 
                805                 810                 815     


Leu Glu Arg Ile Thr Arg Gly Leu Asp Gly Lys Ala Leu Glu Ala Ala 
            820                 825                 830         


Leu Ala Glu Phe Ala Ala Thr Ala Lys Leu Pro Asp Gly Thr Asp Asn 
        835                 840                 845             


Pro Tyr Tyr Gly Leu Arg Arg Val Arg Leu Glu Glu Thr Leu Gln Gln 
    850                 855                 860                 


Gly Ala Arg Val Glu Val Lys Asp Arg Asn Gly Lys Pro Phe Lys Ala 
865                 870                 875                 880 


Tyr Lys Gly Asp Ser Asn His Cys Tyr Glu Ile Trp Arg Met Pro Asp 
                885                 890                 895     


Gly Lys Val Val Pro Gln Val Ile Thr Thr Tyr Glu Ala His Gln Ile 
            900                 905                 910         


Asp Val Glu Lys Lys Pro His Pro Ala Ala Lys Arg Leu Leu Arg Ile 
        915                 920                 925             


Phe Lys Arg Asp Met Val Ala Ile Glu Gln Asp Asp Lys Ala Ile Ile 
    930                 935                 940                 


Gly Tyr Val Gln Ser Met His Val Ala Asn Gly Leu Phe Ile Ala Pro 
945                 950                 955                 960 


His Thr Glu Ala Asn Ala Asp Ala Arg Ser Arg Asp Lys Ser Asp Ser 
                965                 970                 975     


Phe Arg Leu Ile Gln Met Ser Ala Gly Pro Leu Val Lys Ala Asn Ala 
            980                 985                 990         


Arg Arg Val Tyr Val Asp Glu Met  Gly Arg Leu Arg Asp  Pro Gly Ala 
        995                 1000                 1005             


Pro Ser  
    1010 


<210>  480
<211>  1012
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  480

Met Ala Asn Met Arg Tyr Arg Leu Ala Leu Asp Leu Gly Ser Thr Ser 
1               5                   10                  15      


Leu Gly Trp Ala Met Val Arg Leu Asp Ala Gln Asn Glu Thr Cys Ala 
            20                  25                  30          


Val Ile Lys Ala Gly Val Arg Ile Phe Ser Asp Gly Arg Asn Pro Lys 
        35                  40                  45              


Asp Gly Ser Ser Leu Ala Val Ser Arg Arg Glu Ala Arg Ala Met Arg 
    50                  55                  60                  


Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Thr Arg Met Met Ala Thr 
65                  70                  75                  80  


Leu Met Lys His Gly Phe Phe Pro Gln Asp Glu Ala Ala Arg Gln Ala 
                85                  90                  95      


Leu Val Asn Lys Glu Pro Phe Ala Leu Arg Ala Lys Gly Leu Asp His 
            100                 105                 110         


Ala Leu Leu Pro Glu Glu Phe Ala Arg Ala Leu Phe His Ile Asn Gln 
        115                 120                 125             


Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Lys Glu Thr Asp 
    130                 135                 140                 


Ser Ser Ala Leu Lys Lys Ala Ile Gly Gln Leu Arg Glu Ala Met Gln 
145                 150                 155                 160 


Ala Ser Gly Cys Arg Thr Val Gly Glu Trp Leu Cys Ala Arg His Gln 
                165                 170                 175     


Gln Gly Leu Ser Val Arg Ala Arg Tyr Arg Glu Asn Arg Ile Thr Lys 
            180                 185                 190         


Asp Asp Gly Lys Thr Lys Ile Glu Lys Ser Tyr Asp Leu Tyr Ile Asp 
        195                 200                 205             


Arg Ala Met Ile Glu Ala Glu Phe Asp Ala Leu Trp Ala Lys Gln Ser 
    210                 215                 220                 


Glu Leu Asn Pro Glu Leu Phe His Glu Thr Ala Arg Ala Glu Leu Lys 
225                 230                 235                 240 


Asp Cys Leu Leu His Gln Arg Pro Leu Lys Pro Val Lys Pro Gly Arg 
                245                 250                 255     


Cys Thr Leu Leu Pro Asp Glu Glu Arg Ala Pro Leu Ala Leu Pro Ser 
            260                 265                 270         


Gln Gln Arg Phe Arg Ile Tyr Gln Glu Val Asn Asn Leu Arg Leu Leu 
        275                 280                 285             


Arg Glu Gly Leu Asn Glu Glu Pro Leu Thr Leu Thr Gln Arg Asp Gln 
    290                 295                 300                 


Leu Ile Gln Ala Leu Glu Thr Asn Pro Lys Arg Ser Phe Thr Gln Ile 
305                 310                 315                 320 


Gln Lys Leu Leu Gly Phe Ser Gly Gln Phe Asn Leu Gln Asp Asp Lys 
                325                 330                 335     


Arg Thr Glu Leu Lys Gly Asn Ser Thr Ser Ala Met Leu Ser Lys Lys 
            340                 345                 350         


Glu Phe Phe Gly Ser Gln Trp Leu Ser Phe Asp Glu Thr His Gln Asp 
        355                 360                 365             


Glu Ile Val Met Gln Leu Val Ser Glu Glu Asn Glu Ala Arg Leu Ile 
    370                 375                 380                 


Ala Trp Leu Gln Ala Glu Thr Gly Val Asp Glu Ile Thr Ala Glu Arg 
385                 390                 395                 400 


Ile Ala Asn Thr Pro Leu Ala Glu Gly Tyr Gly Ser Leu Ser Ala Lys 
                405                 410                 415     


Ala Leu Glu Lys Ile Leu Pro Ala Leu Arg Ala Asp Val Ile Thr Tyr 
            420                 425                 430         


Asp Lys Ala Val Leu Ala Ala Gly Phe Asp His His Ser His Ile Ser 
        435                 440                 445             


His Ala Ala Thr Gly Glu Ile Leu Pro Asp Leu Pro Tyr Tyr Gly Gln 
    450                 455                 460                 


Tyr Leu Gln Arg His Val Gly Phe Gly Thr Gly Lys Pro Glu Asp Pro 
465                 470                 475                 480 


Ala Glu Lys Arg Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ile Gly 
                485                 490                 495     


Leu Asn Gln Val Arg Thr Val Val Asn Ala Leu Ile Lys Arg Tyr Gly 
            500                 505                 510         


His Pro Ser Glu Val Val Val Glu Leu Ala Arg Asp Leu Lys Gln Ser 
        515                 520                 525             


Gln Glu Gln Arg Lys Glu Glu Gln Ala Gln Gln Ala Lys Asn Gln Arg 
    530                 535                 540                 


Arg Asn Glu Arg Met Arg Gln Asp Ile Ala Gly Leu Leu Asn Thr Ser 
545                 550                 555                 560 


Thr Glu Arg Val Arg Ala Leu Asp Leu Gln Lys Met Ile Leu Trp Glu 
                565                 570                 575     


Glu Leu Ser Phe Asn Val Ala Asp Arg Arg Cys Pro Tyr Ser Gly Val 
            580                 585                 590         


Gln Ile Ser Ala Gln Met Leu Phe Ser Asp Gln Val Glu Ile Glu His 
        595                 600                 605             


Ile Leu Pro Phe Ser Gln Thr Leu Asp Asp Ser Leu Asn Asn Lys Thr 
    610                 615                 620                 


Val Ala Met Arg Gln Ala Asn Arg Ile Lys Gly Asn Arg Thr Pro Ala 
625                 630                 635                 640 


Glu Ala Ala Ala Asp Phe Ala Ala Gln Gly Trp Pro Val Glu Asp Met 
                645                 650                 655     


Leu His Arg Ala Ser Leu Met Pro Lys Gly Lys Arg Tyr Arg Phe Ala 
            660                 665                 670         


Gln Asp Gly Met Ala Gln Trp Leu Arg Glu Asp Lys Gly Phe Leu Ala 
        675                 680                 685             


Arg Ala Leu Asn Asp Thr Arg His Leu Ser Arg Val Ala Arg Glu Tyr 
    690                 695                 700                 


Leu Ser Leu Val Cys Pro Gln Gly Thr Arg Val Ile Pro Gly Arg Met 
705                 710                 715                 720 


Thr Ala Met Leu Arg Ala Lys Phe Gly Leu Asn Asp Ile Leu Gly Leu 
                725                 730                 735     


Asn Gly Glu Lys Asn Arg Asn Asp His Arg His His Ala Val Asp Ala 
            740                 745                 750         


Cys Val Ile Ala Val Thr Asp Gln Ser Met Leu Gln Lys Phe Ala Lys 
        755                 760                 765             


Ala Ser Ala Ser Ala Arg Glu Lys Gln Leu Asn Lys Leu Val Glu Ser 
    770                 775                 780                 


Met Pro Leu Pro Trp Asp Ser Tyr Arg Asp His Val Gln Arg Ala Val 
785                 790                 795                 800 


Glu Arg Ile Trp Val Ser His Lys Pro Asp His Gly His Glu Gly Ala 
                805                 810                 815     


Met His Asn Asp Thr Ala Tyr Gly Leu Leu Gly Gly Gly His Val Gln 
            820                 825                 830         


Val Arg Lys Arg Met Asp Gly Val Arg Thr Arg Glu Thr Ala Ala Leu 
        835                 840                 845             


Lys Val Ile Glu Phe Ala Asp Arg Gln Ala His Pro Arg His Gly Leu 
    850                 855                 860                 


Leu Pro Asn Gly Glu Pro Met Pro Tyr Lys Gly Tyr Lys Gly Asp Ser 
865                 870                 875                 880 


Asn Tyr Cys Met Glu Ile Val Arg Asn Asp Lys Gly Lys Trp Glu Gly 
                885                 890                 895     


Glu Val Val Ser Thr Phe Glu Ala Tyr Gln Leu Val Arg Gln His Gly 
            900                 905                 910         


Leu Ala Arg Leu Arg His Pro Ala Leu Ser Val Ser Gly Arg Pro Leu 
        915                 920                 925             


Val Met Arg Leu Leu Arg Asn Asp Thr Leu Arg Val Ile His Asn Gly 
    930                 935                 940                 


Glu Gln Arg Thr Val Arg Ile Cys Lys Met Thr Ala Glu Gly Val Ile 
945                 950                 955                 960 


Ala Leu Ala Asp Thr Thr Glu Ser Asn Val Asp Ala Arg Thr Arg Thr 
                965                 970                 975     


Lys Asp Leu Ser Tyr Ile Phe Lys Thr Ala Gly Ser Leu Gln Lys Ser 
            980                 985                 990         


Lys Gly Arg Arg Val Ala Ile Ser  Pro Ser Gly Glu Met  Lys Asp Pro 
        995                 1000                 1005             


Gly Phe  Phe Asp 
    1010         


<210>  481
<211>  1016
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  481

Met Glu Val Phe Val Asp Val Ser Ala Arg Gln Leu Gly Ile Asp Leu 
1               5                   10                  15      


Gly Ile Gly Thr Cys Gly Trp Ala Leu Leu Ala Glu Asp Ser Leu Ile 
            20                  25                  30          


Ala Leu Gly Thr Arg Thr Phe Asp Val Pro Glu Thr Asp Lys Glu Arg 
        35                  40                  45              


Ile Pro Thr Asn Gln Ile Arg Arg Gln Ala Arg Gly Met Arg Arg Val 
    50                  55                  60                  


Ile Asp Arg Arg Arg Gln Arg Met Asn Ala Val Arg Arg Leu Leu Ala 
65                  70                  75                  80  


Asp His Gly Leu Leu Ala Asp Gly Gly Lys Leu Gly Leu Ala Thr Val 
                85                  90                  95      


Arg Leu Asp Pro Trp Gln Ala Arg Ala Glu Gly Leu Asp Arg Leu Leu 
            100                 105                 110         


Ser Ala Gly Glu Leu Ala Val Ala Leu Gly His Ile Ala Lys His Arg 
        115                 120                 125             


Gly Phe Arg Ser Asn Ser Lys Arg Asp Arg Gly Ala Asn Ala Pro Lys 
    130                 135                 140                 


Glu Ser Ser Ala Met Leu Gly Ala Ile Ala Gln Thr Gly Glu Arg Leu 
145                 150                 155                 160 


Gln His Trp Arg Thr Val Gly Glu Met Phe Trp Lys Asp Glu Phe Tyr 
                165                 170                 175     


Gln Thr Arg Arg Arg Asn Arg Asp Gly Asp Tyr Ser Arg Thr Val Gln 
            180                 185                 190         


Arg Leu Asp Leu Glu Thr Glu Val His Arg Leu Leu Ala Ala Gln Arg 
        195                 200                 205             


Arg Leu Gly Asn Pro Leu Ala Thr Phe Asp Leu Glu Gln Ser Phe Ile 
    210                 215                 220                 


Asp Val Ala Phe Phe Gln Arg Pro Leu Ala Asp Ser Asp Asp Lys Val 
225                 230                 235                 240 


Gly Pro Cys Pro Phe Glu Pro Ser Glu Lys Arg Ala Ala Arg His Ser 
                245                 250                 255     


Pro Ser Phe Glu Arg Phe Arg Leu Leu Ser Arg Leu Ala Ala Leu Arg 
            260                 265                 270         


Leu Ser Ser Gly Ala Gly Glu Arg Pro Leu Ser Asp Ala Glu Phe Ala 
        275                 280                 285             


Ala Ala Cys Ala Asp Phe Gly Arg Leu Gln Gly Phe Ser Phe Lys Arg 
    290                 295                 300                 


Leu Arg Gln His Leu Lys Leu Asp Pro Ser Ile Arg Phe Ala Gly Ile 
305                 310                 315                 320 


Ala Pro Asp Glu Glu Gly Lys Arg Asp Val Val Ala Arg Ser Gly Glu 
                325                 330                 335     


Ala Thr Pro Gly Ser Ala Ala Leu Arg His Cys Leu Gly Asp Thr Val 
            340                 345                 350         


Trp Asp Asn Met Leu Gly Thr Arg Pro Ala Leu Leu Asp Ala Ile Ala 
        355                 360                 365             


Ala Ile Leu Thr Phe Arg Asp Asp Ala Asp Arg Ile Ala Glu Gly Leu 
    370                 375                 380                 


Ala Gly Leu Gly Ile Glu Glu Pro Ile Leu Asp Ala Leu Met Asp Gly 
385                 390                 395                 400 


Val Arg Asn Gly Ser Phe Gly Lys Phe Lys Gly Pro Gly His Ile Ser 
                405                 410                 415     


Ala Lys Ala Cys Arg Ala Leu Leu Pro His Leu Ala Arg Gly Arg Val 
            420                 425                 430         


Tyr Ser Asp Ala Cys Ala Asp Ala Gly Tyr Asp His Ser Arg Gln Thr 
        435                 440                 445             


Glu Thr Asn Leu Asp Asp Ile Arg Asn Pro Val Ala Arg Lys Ala Leu 
    450                 455                 460                 


Gly Glu Ala Leu Lys Gln Ile Lys Ile Ile Ile His Thr His Gly Arg 
465                 470                 475                 480 


Pro Thr Ala Ile His Val Glu Met Ala Arg Asp Ile Gly Lys Ser Ala 
                485                 490                 495     


Asp Glu Arg Asp Glu Ile Lys Lys Gly Ile Asp Lys Arg Asn Ser Glu 
            500                 505                 510         


Lys Asp Arg Ala Arg Gln Gln Phe His Glu Met Val Gly Thr Pro Ala 
        515                 520                 525             


Ala Gly Ala Glu Asp Leu Leu Arg Phe Glu Leu Trp Lys Glu Gln Asn 
    530                 535                 540                 


Gly Trp Cys Leu Tyr Thr Gly Arg Glu Ile Pro Ile Asp Ala Leu Val 
545                 550                 555                 560 


Ser Ser Asp Asn Arg Ile Gln Val Asp His Ile Leu Pro Trp Ser Arg 
                565                 570                 575     


Ser Gly Asp Asp Ser Tyr Ile Asn Lys Thr Leu Cys Phe Thr Glu Ala 
            580                 585                 590         


Asn Gln His Lys Lys Gly Met Thr Pro Trp Glu Trp Leu His Asp Asp 
        595                 600                 605             


Pro Glu Arg Trp Glu Lys Phe Ala Arg Thr Val Glu Gly Cys Lys Thr 
    610                 615                 620                 


Met Lys Gly Arg Lys Lys Arg Asn Leu Leu Leu Arg Asp Ser Ser Arg 
625                 630                 635                 640 


Leu Glu Lys Glu Phe Arg Ser Arg Asn Leu Asn Asp Thr Arg Tyr Ala 
                645                 650                 655     


Cys Arg Leu Leu Leu Gly Ile Leu Lys His Thr Tyr Pro Asp Ile Val 
            660                 665                 670         


Val Ala Ala Arg Pro Gly Pro Leu Thr Asp Arg Leu Arg Arg Ala Trp 
        675                 680                 685             


Gly Leu Gln Ser Leu Lys Lys His Glu Asp Gly Thr Arg Lys Asp Asp 
    690                 695                 700                 


Asp Arg His His Ala Leu Asp Ala Ala Val Val Ala Ala Thr Ser Gln 
705                 710                 715                 720 


Ser Met Leu Gln Arg Leu Thr Thr Leu Phe Gln Lys Ala Glu Arg Glu 
                725                 730                 735     


Gly Gly Arg Arg Asp Phe Val Gly Leu Glu Pro Pro Trp Pro Gly Phe 
            740                 745                 750         


Arg Ala Asp Leu Glu Ala Lys Leu Glu Gly Val Val Val Ser Arg Ala 
        755                 760                 765             


Glu Arg Arg Arg Ala Arg Gly Ala Gly His Lys Asp Thr Ile Arg Ala 
    770                 775                 780                 


Ile Gly Glu Gly Asp Ala Gly Pro Val Val Tyr Glu Arg Lys Pro Val 
785                 790                 795                 800 


Ala Ala Leu Thr Glu Lys Asp Leu Asp Arg Val Lys Asp Pro Glu Arg 
                805                 810                 815     


Asn Lys Val Leu Ile Glu Asn Leu Arg Gln Trp Ile Ala Ala Gly Lys 
            820                 825                 830         


Pro Lys Asp Pro Asp Lys Ala Pro Arg Ser Pro Lys Gly Asp Pro Met 
        835                 840                 845             


Thr Lys Val Lys Leu Val Thr Thr Asp Asp Val Arg Ile Leu Val Arg 
    850                 855                 860                 


Gly Gly Thr Ala Asp Arg Gly Asp Met Ala Arg Val Asp Val Phe Arg 
865                 870                 875                 880 


Gln Ala Asp Pro Lys Gly Arg Trp Arg Phe Phe Leu Val Pro Val Tyr 
                885                 890                 895     


Pro His Gln Val Phe Asp Pro Thr Asp Trp Pro Gln Pro Pro Asn Arg 
            900                 905                 910         


Ala Val Val Ala Tyr Lys Pro Glu Lys Glu Trp Thr Val Met Gly Pro 
        915                 920                 925             


Asp His Glu Phe Leu Trp Ser Leu Tyr Pro Phe Ser Trp Ile Ala Val 
    930                 935                 940                 


Glu Lys Ala Asp Gly Thr Arg Ile Glu Gly Tyr Phe Arg Gly Met His 
945                 950                 955                 960 


Arg Ser Thr Gly Ala Ile Ser Ile Ser Pro His His Ser Lys Ser Leu 
                965                 970                 975     


Ala Ser Thr Gly Ile Gly Ala Lys Thr Leu Ala Ser Phe Arg Lys Phe 
            980                 985                 990         


Ala Met Asp Arg Met Gly Asn Leu  Ser Glu Ile Glu Arg  Glu Val Arg 
        995                 1000                 1005             


Thr Trp  His Gly Val Ala Cys  Thr 
    1010                 1015     


<210>  482
<211>  1017
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  482

Met Arg Val Asp Ser Leu Gly Arg Cys Leu Ser Asp Glu Glu Trp Ala 
1               5                   10                  15      


Arg Val Ile Tyr His Leu Cys Lys His Arg Gly Phe His Trp Ile Ser 
            20                  25                  30          


Arg Ala Glu Ala Lys Gln Thr Glu Gly Asp Ser Lys Ser Glu Gly Gly 
        35                  40                  45              


Lys Val Lys Gln Gly Leu Ala Gly Thr Ala Lys Leu Met Asp Glu Lys 
    50                  55                  60                  


Gly Tyr Arg Ser Ala Ala Glu Met Val Leu Ala Glu Phe Pro Glu Ala 
65                  70                  75                  80  


Gln Arg Asn Lys Gln Gly Glu Tyr Arg Lys Ala Leu Ser Arg Glu Leu 
                85                  90                  95      


Leu Ala Lys Glu Leu Ala Leu Leu Phe Ala Arg Gln Arg Val Phe Gly 
            100                 105                 110         


Asn Arg His Ala Thr Asp Thr Leu Glu Thr Ala Ile Leu Gly Asn Ser 
        115                 120                 125             


Asp Lys Lys Ser Gly Leu Phe Trp Gln Gln Lys Pro Ala Leu Ala Gly 
    130                 135                 140                 


Ala Asp Leu Leu Lys Met Leu Gly Thr Cys Thr Phe Glu Lys Asn Glu 
145                 150                 155                 160 


Tyr Arg Ala Pro Lys Ala Ser Phe Thr Ala Glu Arg His Val Trp Leu 
                165                 170                 175     


Thr Arg Leu Asn Asn Leu Arg Ile Val Thr Asp Gly Ile Thr Arg Gln 
            180                 185                 190         


Leu Thr Glu Gln Glu Arg Gln Leu Val Leu Pro Leu Pro Tyr Arg Gln 
        195                 200                 205             


Ala Gly Asp Leu Thr Tyr Lys Gln Leu Gly Met Ala Leu Thr Lys Ala 
    210                 215                 220                 


Gly Leu Leu Glu Ala Gly Cys Phe Lys Phe Ile Gly Leu Ala Tyr Pro 
225                 230                 235                 240 


Thr Glu Ala Gln Lys Ala Glu Gly Lys Thr Lys Asp Pro Glu Ser Ala 
                245                 250                 255     


Thr Leu Val Lys Ile Pro Ala Trp Gln Glu Leu Arg Gln Thr Leu Asn 
            260                 265                 270         


Lys Ala Ser Leu Asn Asp Glu Trp Glu Lys Leu Ser Gly Asp Ala Leu 
        275                 280                 285             


Cys Gly Gln Pro Glu Ile Phe Asp Lys Ile Ala Trp Ile Leu Ser Val 
    290                 295                 300                 


Tyr Lys Asp Asp Asp Glu Val Arg Thr Glu Leu Ala Lys Leu Val Leu 
305                 310                 315                 320 


Pro Asn Pro Glu Gly Leu Thr Glu Ala Leu Leu Asn Ile Arg Phe Asp 
                325                 330                 335     


Lys Phe His Ala Leu Ser Leu Lys Ala Leu Arg Ala Ile Val Pro His 
            340                 345                 350         


Met Glu Lys Gly Leu Arg Tyr Asp Glu Ala Cys Val Gln Ala Gly Tyr 
        355                 360                 365             


His His Ser Gln Leu His Lys Ile Gly Glu Gly Glu His Lys Tyr Leu 
    370                 375                 380                 


Pro Pro Leu Tyr Asp Gly Arg Asp Gly Asp Gly Arg Met Ile Phe Ala 
385                 390                 395                 400 


Glu Asp Ala Asp Ile Pro Arg Asn Pro Val Val Leu Arg Ser Leu Asn 
                405                 410                 415     


Gln Ala Arg Lys Val Val Asn Ala Ile Ile Arg Arg Tyr Gly Ser Pro 
            420                 425                 430         


Ser Ala Val His Ile Glu Met Ala Arg Asp Leu Ser Arg Pro Phe Asp 
        435                 440                 445             


Glu Arg Asn Lys Ile Lys Lys Asp Gln Asp Glu Tyr Gln Ala Arg Asn 
    450                 455                 460                 


Gln Arg Asp Arg Glu Gln Phe Ile Glu Leu Phe Gly His Pro Pro Lys 
465                 470                 475                 480 


Gly Leu Glu Phe Glu Lys Trp Arg Leu Tyr Arg Glu Gln Asn Gly Gln 
                485                 490                 495     


Cys Ala Tyr Ser Gln Lys Pro Leu Ala Ser Ser Gly Asp Val Ala Glu 
            500                 505                 510         


Ile Phe Leu Asp Gly Ser Thr Glu Ile Asp His Ala Leu Pro Tyr Ser 
        515                 520                 525             


Arg Ser Phe Asp Asp Ser Lys Asn Asn Lys Val Leu Val Leu Thr Arg 
    530                 535                 540                 


Glu Asn Arg Asp Lys Gly Asn Arg Thr Pro Tyr Glu Tyr Leu Gly Gly 
545                 550                 555                 560 


Ala Glu Asn Ser Glu Arg Trp Gln Arg Phe Phe Ser Phe Val Glu Ser 
                565                 570                 575     


Asn Lys Ala Tyr Arg Leu Ala Lys Arg Thr Arg Leu Leu Arg Lys Asp 
            580                 585                 590         


Phe Gly Ala Lys Glu Ser Glu Glu Phe Arg Glu Arg Asn Leu Asn Asp 
        595                 600                 605             


Thr Arg Tyr Val Cys Arg Phe Phe Lys Asn Tyr Val Glu Gln His Leu 
    610                 615                 620                 


Lys Leu Ala Glu Gly Ser Glu Ala Lys Arg Cys Val Val Val Ser Gly 
625                 630                 635                 640 


Gln Leu Thr Asn Phe Leu Arg Thr Arg Trp Gly Leu Leu Lys Ile Arg 
                645                 650                 655     


Ser Asp Ser Asp Arg His His Ala Leu Asp Ala Ala Val Val Ala Ala 
            660                 665                 670         


Cys Ser His Ser Met Val Gln Arg Leu Ser Asn Tyr Ser Arg Thr Arg 
        675                 680                 685             


Glu Leu Glu Gln Val Arg Asp Gly Phe Val Asn Ile Glu Thr Gly Glu 
    690                 695                 700                 


Ile Val Asn Pro Ala Met His Gln Gln Leu Arg Glu His Phe Pro Asp 
705                 710                 715                 720 


Pro Trp Pro Tyr Phe Arg His Glu Leu Glu Ala Arg Leu Lys Ile Asp 
                725                 730                 735     


Ser Pro Ala Leu Leu Arg Glu Glu Met Ala Arg Leu Ser Ser Tyr Thr 
            740                 745                 750         


Glu Glu Glu Leu Ala Ala Leu Lys Pro Leu Phe Val Ser Arg Ala Pro 
        755                 760                 765             


Gln Arg Arg Asn Gly Gly Ala Ala His Lys Asp Thr Ile Tyr Gly Gln 
    770                 775                 780                 


Pro Glu Lys Leu Lys Lys Asn Gly Ser Val Ser Gln Lys Val Ala Leu 
785                 790                 795                 800 


Ala Ser Leu Ala Leu Lys Asp Ile Asp Leu Leu Ala Asp Pro His Arg 
                805                 810                 815     


Asn Ala Lys Leu Tyr Gly Ala Ile Arg Glu Arg Leu Glu Ala His Gly 
            820                 825                 830         


Gly Lys Gly Asp Lys Ala Phe Pro Ala Thr Asn Pro Leu Arg Lys Pro 
        835                 840                 845             


Asp Lys Glu Gly Asn Pro Thr Gly Pro Ile Val Arg Thr Val Thr Lys 
    850                 855                 860                 


Val Ile Asp Lys Leu Ser Gly Ile Pro Val Arg Gly Gly Ile Ala Lys 
865                 870                 875                 880 


Asn Asp Thr Met Leu Arg Val Asp Val Phe Thr Lys Ala Gly Arg Phe 
                885                 890                 895     


His Leu Val Pro Val Tyr Val His His Arg Val Thr Gly Leu Pro Asn 
            900                 905                 910         


Gln Ala Ile Thr Arg Gly Lys Asp Glu Asn Gln Trp Ala Ile Ile Asp 
        915                 920                 925             


Asp Thr Phe Glu Trp Arg Phe Ser Leu Tyr Pro Asn Asp Leu Val Gln 
    930                 935                 940                 


Ile Arg Leu Lys Asp Glu Thr His Leu Gly Tyr Tyr Ala Ser Thr Asp 
945                 950                 955                 960 


Arg Ser Thr Ser Ser Ile Ser Ile Trp Ala His Asp Arg Asn Gln Ser 
                965                 970                 975     


Val Gly Lys Asp Gly Leu Ile Arg Ser Ile Gly Val Thr Thr Ala Lys 
            980                 985                 990         


Asp Ile Glu Lys Phe His Val Asp  Thr Leu Gly Asn Val  Tyr Pro Ala 
        995                 1000                 1005             


Pro Pro  Glu Lys Arg Arg Gly  Leu Ala 
    1010                 1015         


<210>  483
<211>  1021
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  483

Met Arg Arg Arg Arg Asp Arg Phe Lys Gln Arg Gln Ala Ala Leu Leu 
1               5                   10                  15      


Lys His Leu Ala Gly Val Gly Leu Phe Pro Ala Asp Glu Ala Ala Arg 
            20                  25                  30          


Arg Glu Leu Ala Gly Leu Asp Pro Tyr Glu Leu Arg Ala Arg Ala Leu 
        35                  40                  45              


His Glu Ala Leu Pro Leu Asn His Ile Gly Arg Ala Leu Phe His Leu 
    50                  55                  60                  


Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Arg Gly Lys 
65                  70                  75                  80  


Ala Asp Asp Glu Gly Lys Ile Arg Leu Gly Val Ala Arg Leu Gln Glu 
                85                  90                  95      


Ala Met His Glu Lys Gly Ser Ala Thr Leu Gly Glu Phe Leu His Gly 
            100                 105                 110         


Arg Arg Ala Ala Ala Thr Asp Pro Asn Gln Ile Pro Ser Val Arg Thr 
        115                 120                 125             


Arg Leu Arg Ala Glu Ser Gly Glu Gly Ala Arg Gly Asp Gly Tyr Asp 
    130                 135                 140                 


Phe Tyr Pro Asp Arg Arg Met Leu Glu Asp Glu Phe Glu Ala Ile Trp 
145                 150                 155                 160 


Glu Ser Gln Gln Val His His Pro Ala Val Leu Thr Asp Glu Ile Lys 
                165                 170                 175     


Gln Arg Phe Phe Glu Val Ile Phe Tyr Gln Arg Pro Leu Lys Gln Pro 
            180                 185                 190         


Glu Ile Gly Thr Cys Thr Leu Val Ala Gly Glu Arg Arg Leu Pro Lys 
        195                 200                 205             


Ala His Pro Leu Phe Gln Lys Arg Arg Leu Leu Glu Glu Leu Asn Ala 
    210                 215                 220                 


Leu Arg Ile Val Trp Pro Gly Val Gln Ala Glu Leu Leu Ser Arg Glu 
225                 230                 235                 240 


Gln Arg Asp Leu Ala Tyr Leu Lys Leu Lys Asp Lys Arg Ala Val Arg 
                245                 250                 255     


Phe Glu Ser Leu Arg Lys Ala Leu Lys Leu Asp Pro Asp Ala Arg Phe 
            260                 265                 270         


Asn Lys Glu Ser Glu Asn Arg Ser Glu Leu Lys Gly Asp Glu Val Ala 
        275                 280                 285             


Ala Glu Leu Gly Ala Lys Ser Arg Phe Gly Gly Arg Trp Gly His Thr 
    290                 295                 300                 


Ala Pro Ala Asp Gln Trp Glu Ile Val Ser Arg Ile Gln Ala Leu Glu 
305                 310                 315                 320 


Ser Asp Asp Asp Glu Ala Gln Phe Cys Val Trp Leu Ser Glu Arg Tyr 
                325                 330                 335     


Gly Leu Asp Ser Glu Gln Ala Arg Ala Val Ala Ala Ala Arg Leu Pro 
            340                 345                 350         


Gln Gly Phe Gly Arg Phe Gly Leu Thr Ala Thr Thr Arg Leu Ile Lys 
        355                 360                 365             


Ala Leu Gln Asp Glu Val Ile Val Tyr Ser Glu Ala Val Gln Lys Thr 
    370                 375                 380                 


Gly Leu Gly His His Ser Asp Phe Arg Asn Gly Glu Val Leu Ala Glu 
385                 390                 395                 400 


Leu Pro Tyr Tyr Gly Ile Ala Leu Glu Arg His Ile Met Pro Gly Thr 
                405                 410                 415     


Gly Asp Pro Gly Asp Ser Asp Glu Met Arg Val Gly Arg Val Thr Asn 
            420                 425                 430         


Pro Thr Val His Ile Gly Leu Asn Gln Leu Arg Arg Val Val Asn Arg 
        435                 440                 445             


Leu Ile Lys Arg Tyr Gly Pro Pro Arg Gln Ile Ala Val Glu Leu Ala 
    450                 455                 460                 


Arg Glu Leu Lys Leu Asp Asp Gln His Lys Arg Glu Ile Asn Gly Arg 
465                 470                 475                 480 


Asn Arg Gln Asn Arg Glu Ala Ala Glu Arg Arg Ser Gln Lys Leu Val 
                485                 490                 495     


Asp Ile Gly Ile Ala Asp Lys Gly Ala Asn Arg Ala Leu Leu Lys Leu 
            500                 505                 510         


Trp Glu Glu Leu Asn Pro Asp Asn Ala Leu Asp Arg Arg Cys Val Tyr 
        515                 520                 525             


Thr Gly Arg Gln Ile Ser Ile Glu Met Leu Phe Ser Gly Ala Val Glu 
    530                 535                 540                 


Val Asp His Ile Leu Pro Phe Ala Ala Thr Leu Asp Asp Ser Asn Ala 
545                 550                 555                 560 


Asn Lys Leu Leu Cys Leu Arg Glu Ala Asn Arg Asn Lys Arg Arg Arg 
                565                 570                 575     


Ser Pro Phe Glu Ala Phe Gly Gln Gly Ala Asp Trp Glu Glu Ile Ala 
            580                 585                 590         


Ala Arg Ala Ala Arg Leu Pro Arg Glu Lys Arg Trp Arg Phe Glu Pro 
        595                 600                 605             


Asp Ala Met Ala Arg Phe Asp Thr Glu Glu Lys Phe Leu Ala Arg Gln 
    610                 615                 620                 


Leu Val Asp Thr Gln Tyr Leu Ser Arg Leu Ala Arg Glu Tyr Leu Gly 
625                 630                 635                 640 


Val Leu Phe Pro Asp Lys Gly Glu Gly Ser Gly Arg Val Trp Val Ser 
                645                 650                 655     


Pro Gly Arg Leu Thr Glu Met Leu Arg Arg Ser Trp Gly Leu Asn His 
            660                 665                 670         


Leu Leu Pro Asp His Asn Leu Gly Gly Gly Ala Asp Gln Pro Lys Asn 
        675                 680                 685             


Arg Arg Asp His Arg His His Ala Ile Asp Ala Ile Val Val Ala Val 
    690                 695                 700                 


Thr Asp Arg His Leu Leu Gln Ala Ile Ala Arg Glu Ala Gly Gln Arg 
705                 710                 715                 720 


Gly His Glu Ala Ala Asn Arg Met Thr Ala Asp Leu Pro Val Pro Trp 
                725                 730                 735     


Pro Asp Phe Asp Arg Asp Val Gly Gln Val Ile Arg Gln Val Val Val 
            740                 745                 750         


Ser His Arg Ala Asp His Gly Thr Val Ser Lys Ala Gly Ala Ala Lys 
        755                 760                 765             


Gly Gln Asp Ser Thr Ala Gly Arg Leu His Asn Asp Thr Ala Tyr Gly 
    770                 775                 780                 


Leu Thr Gly Gln Thr Asp Glu Lys Gly Asn Thr Ile Val Val Arg Arg 
785                 790                 795                 800 


Val Pro Leu Thr Ala Leu Lys Lys Pro Thr Ser Ile Glu Ala Val Arg 
                805                 810                 815     


Asp Asp Glu Leu Arg Lys Ala Leu Ser Val Trp Thr Arg Gly Tyr Asp 
            820                 825                 830         


Gly Lys Ala Phe Glu Ala Arg Leu Ser Arg Phe Pro Glu Leu Gly Pro 
        835                 840                 845             


Leu Gln Phe Arg Gly Ile Arg Arg Val Arg Val Val Glu Pro Leu Thr 
    850                 855                 860                 


Val Ile Pro Ile Arg Asp Ser Ser Gly Arg Val Tyr Lys Gly Tyr Lys 
865                 870                 875                 880 


Gly Asp Ser Asn Tyr Arg Tyr Asp Val Trp Glu Leu Lys Asp Gly Lys 
                885                 890                 895     


Trp Val Ala Glu Val Val Ser Met Phe Asp Ala His Gln Ser Ser Trp 
            900                 905                 910         


Thr Ser Pro Val Arg Arg Glu Asn Pro Thr Ala Arg Lys Val Leu Ser 
        915                 920                 925             


Leu Gln Gln Asn Asp Ile Val Ala Ile Glu Pro Glu Gly Lys Glu Arg 
    930                 935                 940                 


Glu Leu Met Arg Val Ala Gln Leu Arg Gln Gly Gly Gln Ile Thr Leu 
945                 950                 955                 960 


Ala Ala Cys His Glu Ala Gly Asp Leu Lys Arg Arg Asp Ala Glu Ser 
                965                 970                 975     


Asn Glu Val Asp Pro Phe Lys Tyr Phe Ser Pro Thr Ala Gly Gly Leu 
            980                 985                 990         


Lys Arg Val Arg Ala Arg Gln Val  Arg Val Asp Glu Leu  Gly Arg Val 
        995                 1000                 1005             


Leu Asp  Pro Gly Ser Arg Asp  Arg Glu Arg Gly Ala  Ala 
    1010                 1015                 1020     


<210>  484
<211>  1021
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  484

Met Lys Tyr Thr Leu Gly Leu Asp Val Gly Ile Ala Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asp Lys Asp Asn Asn Lys Ile Ile Asp Leu Gly Val Arg 
            20                  25                  30          


Cys Phe Asp Lys Ala Glu Glu Ser Lys Thr Gly Glu Ser Leu Ala Thr 
        35                  40                  45              


Ala Arg Arg Ile Ala Arg Gly Met Arg Arg Arg Ile Ser Arg Arg Ser 
    50                  55                  60                  


Gln Arg Leu Arg Leu Val Lys Lys Leu Phe Val Gln Tyr Glu Ile Ile 
65                  70                  75                  80  


Lys Asp Ser Ser Glu Phe Asn Arg Ile Phe Asp Thr Ser Arg Asp Gly 
                85                  90                  95      


Trp Lys Asp Pro Trp Glu Leu Arg Tyr Asn Ala Leu Ser Arg Ile Leu 
            100                 105                 110         


Lys Pro Tyr Glu Leu Val Gln Val Leu Thr His Ile Thr Lys Arg Arg 
        115                 120                 125             


Gly Phe Lys Ser Asn Arg Lys Glu Asp Leu Ser Thr Thr Lys Glu Gly 
    130                 135                 140                 


Val Val Ile Thr Ser Ile Lys Asn Asn Ser Glu Met Leu Arg Thr Lys 
145                 150                 155                 160 


Asn Tyr Arg Thr Ile Gly Glu Met Ile Phe Met Glu Thr Pro Glu Asn 
                165                 170                 175     


Ser Asn Lys Arg Asn Lys Val Asp Glu Tyr Ile His Thr Ile Ala Arg 
            180                 185                 190         


Glu Asp Leu Leu Asn Glu Ile Lys Tyr Ile Phe Ser Ile Gln Arg Lys 
        195                 200                 205             


Leu Gly Ser Pro Phe Val Thr Glu Lys Leu Glu His Asp Phe Leu Asn 
    210                 215                 220                 


Ile Trp Glu Phe Gln Arg Pro Phe Ala Ser Gly Asp Ser Ile Leu Ser 
225                 230                 235                 240 


Lys Val Gly Lys Cys Thr Leu Leu Lys Glu Glu Leu Arg Ala Pro Thr 
                245                 250                 255     


Ser Cys Tyr Thr Ser Glu Tyr Phe Gly Leu Leu Gln Ser Ile Asn Asn 
            260                 265                 270         


Leu Val Leu Val Glu Asp Asn Asn Thr Leu Thr Leu Asn Asn Asp Gln 
        275                 280                 285             


Arg Ala Lys Ile Ile Glu Tyr Ala His Phe Lys Asn Glu Ile Lys Tyr 
    290                 295                 300                 


Ser Glu Ile Arg Lys Leu Leu Asp Ile Glu Pro Glu Ile Leu Phe Lys 
305                 310                 315                 320 


Ala His Asn Leu Thr His Lys Asn Pro Ser Gly Asn Asn Glu Ser Lys 
                325                 330                 335     


Lys Phe Tyr Glu Met Lys Ser Tyr His Lys Leu Lys Ser Thr Leu Pro 
            340                 345                 350         


Thr Asp Ile Trp Gly Lys Leu His Ser Asn Lys Glu Ser Leu Asp Asn 
        355                 360                 365             


Leu Phe Tyr Cys Leu Thr Val Tyr Lys Asn Asp Asn Glu Ile Lys Asp 
    370                 375                 380                 


Tyr Leu Gln Ala Asn Asn Leu Asp Tyr Leu Ile Glu Tyr Ile Ala Lys 
385                 390                 395                 400 


Leu Pro Thr Phe Asn Lys Phe Lys His Leu Ser Leu Val Ala Met Lys 
                405                 410                 415     


Arg Ile Ile Pro Phe Met Glu Lys Gly Tyr Lys Tyr Ser Asp Ala Cys 
            420                 425                 430         


Asn Met Ala Glu Leu Asp Phe Thr Gly Ser Ser Lys Leu Glu Lys Cys 
        435                 440                 445             


Asn Lys Leu Thr Val Glu Pro Ile Ile Glu Asn Val Thr Asn Pro Val 
    450                 455                 460                 


Val Ile Arg Ala Leu Thr Gln Ala Arg Lys Val Ile Asn Ala Ile Ile 
465                 470                 475                 480 


Gln Lys Tyr Gly Leu Pro Tyr Met Val Asn Ile Glu Leu Ala Arg Glu 
                485                 490                 495     


Ala Gly Met Thr Arg Gln Asp Arg Asp Asn Leu Lys Lys Glu His Glu 
            500                 505                 510         


Asn Asn Arg Lys Ala Arg Glu Lys Ile Ser Asp Leu Ile Arg Gln Asn 
        515                 520                 525             


Gly Arg Val Ala Ser Gly Leu Asp Ile Leu Lys Trp Arg Leu Trp Glu 
    530                 535                 540                 


Asp Gln Gly Gly Arg Cys Ala Tyr Ser Gly Lys Pro Ile Pro Val Cys 
545                 550                 555                 560 


Asp Leu Leu Asn Asp Ser Leu Thr Gln Ile Asp His Ile Tyr Pro Tyr 
                565                 570                 575     


Ser Arg Ser Met Asp Asp Ser Tyr Met Asn Lys Val Leu Val Leu Thr 
            580                 585                 590         


Asp Glu Asn Gln Asn Lys Arg Ser Tyr Thr Pro Tyr Glu Val Trp Gly 
        595                 600                 605             


Ser Thr Glu Lys Trp Glu Asp Phe Glu Ala Arg Ile Tyr Ser Met His 
    610                 615                 620                 


Leu Pro Gln Ser Lys Glu Lys Arg Leu Leu Asn Arg Asn Phe Ile Thr 
625                 630                 635                 640 


Lys Asp Leu Asp Ser Phe Ile Ser Arg Asn Leu Asn Asp Thr Arg Tyr 
                645                 650                 655     


Ile Ser Arg Phe Leu Lys Asn Tyr Ile Glu Ser Tyr Leu Gln Phe Ser 
            660                 665                 670         


Asn Asp Ser Pro Lys Ser Cys Val Val Cys Val Asn Gly Gln Cys Thr 
        675                 680                 685             


Ala Gln Leu Arg Ser Arg Trp Gly Leu Asn Lys Asn Arg Glu Glu Ser 
    690                 695                 700                 


Asp Leu His His Ala Leu Asp Ala Ala Val Ile Ala Cys Ala Asp Arg 
705                 710                 715                 720 


Lys Ile Ile Lys Glu Ile Thr Asn Tyr Tyr Asn Glu Arg Glu Asn His 
                725                 730                 735     


Asn Tyr Lys Val Lys Tyr Pro Leu Pro Trp His Ser Phe Arg Gln Asp 
            740                 745                 750         


Leu Met Glu Thr Leu Ala Gly Val Phe Ile Ser Arg Ala Pro Arg Arg 
        755                 760                 765             


Lys Ile Thr Gly Pro Ala His Asp Glu Thr Ile Arg Ser Pro Lys His 
    770                 775                 780                 


Phe Asn Lys Gly Leu Thr Ser Val Lys Ile Pro Leu Thr Thr Val Thr 
785                 790                 795                 800 


Leu Glu Lys Leu Glu Thr Met Val Lys Asn Thr Lys Gly Gly Ile Ser 
                805                 810                 815     


Asp Lys Ala Val Tyr Asn Val Leu Lys Asn Arg Leu Ile Glu His Asn 
            820                 825                 830         


Asn Lys Pro Leu Lys Ala Phe Ala Glu Lys Ile Tyr Lys Pro Leu Lys 
        835                 840                 845             


Asn Gly Thr Asn Gly Ala Ile Ile Arg Ser Ile Arg Val Glu Thr Pro 
    850                 855                 860                 


Ser Tyr Thr Gly Val Phe Arg Asn Glu Gly Lys Gly Ile Ser Asp Asn 
865                 870                 875                 880 


Ser Leu Met Val Arg Val Asp Val Phe Lys Lys Lys Asp Lys Tyr Tyr 
                885                 890                 895     


Leu Val Pro Ile Tyr Val Ala His Met Ile Lys Lys Glu Leu Pro Ser 
            900                 905                 910         


Lys Ala Ile Val Pro Leu Lys Pro Glu Ser Gln Trp Glu Leu Ile Asp 
        915                 920                 925             


Ser Thr His Glu Phe Leu Phe Ser Leu Tyr Gln Asn Asp Tyr Leu Val 
    930                 935                 940                 


Ile Lys Thr Lys Lys Gly Ile Thr Glu Gly Tyr Tyr Arg Ser Cys His 
945                 950                 955                 960 


Arg Gly Thr Gly Ser Leu Ser Leu Met Pro His Phe Ala Asn Asn Lys 
                965                 970                 975     


Asn Val Lys Ile Asp Ile Gly Val Arg Thr Ala Ile Ser Ile Glu Lys 
            980                 985                 990         


Tyr Asn Val Asp Ile Leu Gly Asn  Lys Ser Ile Val Lys  Gly Glu Pro 
        995                 1000                 1005             


Arg Arg  Gly Met Glu Lys Tyr  Asn Ser Phe Lys Ser  Asn 
    1010                 1015                 1020     


<210>  485
<211>  1023
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  485

Met Lys Ile Leu Gly Phe Asp Ile Gly Val Ala Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Gly Asp Glu Ile Arg Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Glu Ala Arg Gly Val Arg Arg Arg Leu Ala Arg Arg Arg Val Arg Leu 
    50                  55                  60                  


Asn Thr Ile Lys Lys Ile Ile Cys Lys Glu Phe Asp Leu Arg Leu Ser 
65                  70                  75                  80  


Asp Tyr Ile Ala Asp Asp Gly Glu Leu Pro Lys Ala Tyr Gln Thr Asn 
                85                  90                  95      


Lys Asp Thr Lys Ser Pro Tyr Glu Leu Arg Tyr Lys Ala Leu Asn Glu 
            100                 105                 110         


Arg Leu Ser Lys Glu Glu Leu Ala Arg Val Ile Leu His Ile Ala Lys 
        115                 120                 125             


His Arg Gly Tyr Gly Asn Lys His Ala Lys Asp Gly Asp Asp Lys Asp 
    130                 135                 140                 


Ser Gly Lys Val Lys Lys Ala Ile Gly Glu Asn Lys Thr Leu Leu Glu 
145                 150                 155                 160 


Gln Lys Gly Tyr Glu Ser Val Gly Glu Tyr Cys Phe Arg Glu Phe Phe 
                165                 170                 175     


Lys Ser Ile Lys Asp Lys Asp Gly Glu Thr His Asn Ile Pro Ser Gln 
            180                 185                 190         


Asn Ile Arg Asn Lys Lys Gly Asn Tyr Asp Arg Cys Val Ser Gln Lys 
        195                 200                 205             


Gln Leu Glu Asp Glu Leu Lys Leu Ile Phe Ala Lys Gln Arg Lys Leu 
    210                 215                 220                 


Gly Phe Asn Lys Glu Phe Glu Asp Ala Ile Leu Glu Val Ala Phe Tyr 
225                 230                 235                 240 


Gln Arg Pro Leu Lys Asp Phe Asp Asp Lys Ile Gly Asp Cys Ile Phe 
                245                 250                 255     


Phe Asp Asn Glu Lys Arg Ala Pro Lys Glu Ser Ile Ser Ala Ile Glu 
            260                 265                 270         


Phe Val Ala Leu Thr Arg Ile Ile Asn Thr Leu Lys Tyr Leu Ser Lys 
        275                 280                 285             


Thr Thr Gly Glu Val Tyr Glu Lys Glu Ile Ile Asn Lys Ile Trp Gln 
    290                 295                 300                 


Lys Ile Leu Glu Asn Gly Lys Met Thr Tyr Ala Glu Leu Arg Lys Ile 
305                 310                 315                 320 


Leu Ala Leu Asp Ser Ser Val Phe Phe Lys Asp Lys Asp Leu Asp Tyr 
                325                 330                 335     


Thr Leu Gly Ile Lys Glu Ala Glu Lys Ile Lys Phe Ile Glu Phe Lys 
            340                 345                 350         


Lys Leu Ile Lys Phe Lys Lys Ala Leu Gly Ser Phe Asp Gly Leu Ser 
        355                 360                 365             


Arg Glu Ile Leu Asp Asn Ile Ala Met Gln Ile Ala Ile Thr Lys Asp 
    370                 375                 380                 


Arg Gln Arg Leu Leu Glu Lys Leu Glu Val Phe Pro Leu Asn Lys Glu 
385                 390                 395                 400 


Gln Lys Glu Asn Leu Ser Gln Leu Asn Phe Ser Lys Tyr Ile Asp Leu 
                405                 410                 415     


Ser Leu Lys Ala Leu Asn Gln Ile Leu Pro Phe Met Arg Glu Gly Arg 
            420                 425                 430         


Arg Tyr Asp Glu Ala Lys Asp Asp Ala Lys Leu Gln Glu Lys Ala Lys 
        435                 440                 445             


Asn Thr Lys Lys Glu Glu Phe Leu Pro Pro Leu Lys Glu Phe Asp Leu 
    450                 455                 460                 


Asn Leu Thr Asn Pro Val Val Ala Arg Ala Leu Ala Glu Phe Arg Lys 
465                 470                 475                 480 


Val Leu Asn Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile His 
                485                 490                 495     


Leu Glu Phe Ala Arg Asp Met Arg Leu Asp Phe Asp Glu Arg Glu Arg 
            500                 505                 510         


Leu Ile Ser Ile Gln Asn Asp Asn Phe Asn Lys Arg Gln Leu Ala Glu 
        515                 520                 525             


Asp Phe Cys Lys Glu His Gly Leu Glu Pro Ser Asn Ala Asn Ile Leu 
    530                 535                 540                 


Lys Ile Lys Leu Trp Gln Glu Gln Gln Trp His Cys Met Tyr Ser Gly 
545                 550                 555                 560 


Glu Leu Ile Gly Ile Pro Asp Leu Lys Asp Pro Thr Lys Leu Gln Val 
                565                 570                 575     


Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Thr Asn 
            580                 585                 590         


Lys Val Leu Val Arg Ala Glu Trp Asn Gln Glu Lys Gly Asn His Thr 
        595                 600                 605             


Pro Phe Glu Ala Phe Gly Gly Asp Lys Asn Lys Trp Asp Lys Ile Val 
    610                 615                 620                 


Ala Leu Ala Glu Lys Leu Pro Pro Ser Lys Lys Leu Asn Arg Ser Glu 
625                 630                 635                 640 


Lys Ser Lys Lys Asp Lys Leu Leu Asp Glu Lys Phe Asn Asp Lys Glu 
                645                 650                 655     


Leu Gly Phe Leu Ala Arg Asn Ile Val Asp Thr Gly Tyr Ile Ala Arg 
            660                 665                 670         


Leu Val Ala Lys Tyr Ile Asp Met Tyr Leu Glu Phe Leu Pro Leu Glu 
        675                 680                 685             


Glu Asn Glu Asn Thr Ser Leu Ala Arg Gly Glu Lys Gly Ser Lys Lys 
    690                 695                 700                 


His Ile Gly Ile Val Asn Gly Arg Leu Thr Ser Glu Val Arg His Asn 
705                 710                 715                 720 


Trp Gly Leu Asp Pro Lys Asp Arg Asp Asn His Leu His His Ala Ile 
                725                 730                 735     


Asp Ala Ile Val Ile Ala Tyr Met Asn Asp Lys Arg Ile Lys Ala Phe 
            740                 745                 750         


Ala Asp Phe Arg Arg Leu Lys Glu Leu Lys Ile Lys Arg Tyr Gly Arg 
        755                 760                 765             


Glu Ile Thr Glu Asp Glu Tyr Gln Glu Lys Lys Lys Glu Leu Ser Cys 
    770                 775                 780                 


Ala Pro Phe Asp Asp Phe Arg Asn Arg Thr Leu Glu Asn Ile Asp Lys 
785                 790                 795                 800 


Ile Phe Val Ser Arg Pro Pro Arg Lys Arg Ala Arg Gly Thr Leu His 
                805                 810                 815     


Glu Glu Thr Phe Tyr Ser Ile Asp Asp Lys Lys Leu Leu Lys Ala Tyr 
            820                 825                 830         


Gly Gly Lys Glu Gly Met Gln Arg Ala Leu Ser Leu Gly Lys Ile Arg 
        835                 840                 845             


Gln Ile Gly Thr Lys Ile Val Lys Asn Gly Thr Met Val Arg Val Asp 
    850                 855                 860                 


Ile Phe Lys Asp Lys Arg Gly Arg Phe Tyr Gly Val Pro Ile Tyr Thr 
865                 870                 875                 880 


Met Asp Phe Ala Leu Gly Val Leu Pro Asn Lys Ala Ala Val Ser Gly 
                885                 890                 895     


Lys Asp Lys Asn Gly Leu Ile Lys Glu Trp Ala Glu Met Asp Ser Gly 
            900                 905                 910         


Tyr Glu Phe Cys Phe Ser Leu Phe Lys Asp Asp Leu Ile Leu Val Gln 
        915                 920                 925             


Arg Lys Glu Met Gln Glu Pro Glu Leu Cys Tyr Phe Val Ser Phe Asp 
    930                 935                 940                 


Ser Ser Asn Ala Gln Ile Asp Val Arg Ser His Asn Asn His Phe Ser 
945                 950                 955                 960 


Val Leu Asn Glu Asn Gln Lys Leu Leu Phe Lys Ser Ala Thr Gln Ala 
                965                 970                 975     


Glu Val Ile Gly Glu Ser Ile Gly Ile Gln Asn Leu Lys Val Phe Glu 
            980                 985                 990         


Lys Trp His Val Ser Pro Leu Gly  Glu Val Arg Lys Ala  Glu Phe Ser 
        995                 1000                 1005             


Pro Arg  Gly Glu Ala Lys Leu  Lys Thr Ser Lys Lys  Arg Lys Lys 
    1010                 1015                 1020             


<210>  486
<211>  1023
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  486

Met Lys Ile Tyr Ser Phe Asp Ile Gly Val Ala Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Ile Glu Asn Glu Val Leu Gln Asp Cys Gly Ile Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Asn Gly Ala Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Glu Ala Arg Gly Val Arg Lys Arg Leu Ala Arg Arg Arg Gly Arg Leu 
    50                  55                  60                  


Asn Thr Ile Lys Gln Leu Leu Cys Lys Glu Phe Gly Leu Gln Leu Glu 
65                  70                  75                  80  


Asp Tyr Leu Ser Asn Asp Gly Glu Leu Pro Lys Ala Tyr Thr Ala Ser 
                85                  90                  95      


Lys Ala Asn Pro Leu Lys Ser Pro Tyr Glu Leu Arg Thr Leu Ala Leu 
            100                 105                 110         


Ser Gln Lys Leu Asp Ser Lys Asp Leu Val Arg Val Ile Leu His Ile 
        115                 120                 125             


Ala Lys His Arg Gly Tyr Gly Asn Lys His Ala Lys Asp Ser Lys Asp 
    130                 135                 140                 


Thr Glu Ser Gly Lys Val Lys Lys Ala Ile Glu Phe Asn Arg Lys Thr 
145                 150                 155                 160 


Leu Ala Glu Lys Gly Tyr Arg Ser Val Gly Glu Tyr Leu Tyr Arg Glu 
                165                 170                 175     


Phe Phe Gln Gln Ser Arg Ile Ser Gln Asn Asn Gly Ala Thr Glu Phe 
            180                 185                 190         


Ile Asn Val Arg Asn Lys Ala Gly Asn Tyr Glu His Cys Val Ala Gln 
        195                 200                 205             


Asp Met Leu Lys Glu Glu Leu Glu Ser Ile Leu Asn Ala Gln Arg Gln 
    210                 215                 220                 


Phe Gly Phe Ser Leu Lys Gln Asp Phe Glu Ser Lys Leu Leu Thr Lys 
225                 230                 235                 240 


Ile Phe Glu Gln Arg Pro Leu Lys Ser Phe Ala Asp Lys Val Gly Asn 
                245                 250                 255     


Cys Cys Phe Ile Ala Gly Glu Lys Arg Ala Pro Lys Asp Ser Pro Ser 
            260                 265                 270         


Ala Ile Glu Phe Ile Ala Leu Ser Arg Thr Ile Asn Thr Leu Ile Asn 
        275                 280                 285             


Leu Ser Lys Glu Ser Gly Glu Ile Tyr Asn Arg Ala Ile Ile Leu Gln 
    290                 295                 300                 


Ile Val Glu Ser Thr Leu Lys Lys Gly Glu Ile Ser Tyr Lys Glu Leu 
305                 310                 315                 320 


Arg Glu Ile Ile Arg Leu Asp Glu Arg Leu Arg Phe Lys Asp Pro Arg 
                325                 330                 335     


Leu Asp Tyr Ser Arg Glu Ile Lys Glu Ala Glu Lys Val Lys Phe Leu 
            340                 345                 350         


Glu Phe Lys Gln Leu Lys Gly Phe Lys Lys Ala Leu Gly Glu Ser Phe 
        355                 360                 365             


Thr Thr Leu Ala Ile Ser Gln Leu Asp Glu Ile Ala Glu His Ile Ala 
    370                 375                 380                 


Leu Ile Lys Asp Arg Val Lys Leu Asn Glu Lys Leu Glu Ser Tyr Ala 
385                 390                 395                 400 


Leu Thr Lys Glu Gln Arg Glu Ala Leu Ser Asn Leu Ser Phe Ser Ala 
                405                 410                 415     


His Ile Asn Leu Ser Leu Gln Ala Leu Lys Gln Ile Leu Pro Phe Met 
            420                 425                 430         


Arg Gly Asp Asn Gly Glu Gln Cys Leu Asp Tyr Asp Glu Ser Val Glu 
        435                 440                 445             


Lys Ala Gly Leu Lys Ala Leu Ser Lys Asp Thr Leu Lys Gly Gln Met 
    450                 455                 460                 


Leu Pro Pro Leu Asn Glu Phe Glu Pro Tyr Leu Ser Asn Pro Val Val 
465                 470                 475                 480 


Ala Arg Ala Leu Ala Glu Tyr Arg Lys Val Leu Asn Val Leu Leu Lys 
                485                 490                 495     


Gln Tyr Gly Ser Pro His Lys Ile His Ile Glu Tyr Ala Arg Glu Ala 
            500                 505                 510         


Lys Leu Ser Ser Thr Glu Arg Gln Lys Tyr Glu Lys Glu Gln Lys Asp 
        515                 520                 525             


Asn Tyr Ala Ala Asn Gln Ala Ala His Lys Lys Cys Gln Glu Leu Gly 
    530                 535                 540                 


Leu Glu Pro Ser Ser Thr Asn Leu Leu Lys Leu Lys Leu Trp Gln Glu 
545                 550                 555                 560 


Gln Gly Gly Leu Cys Leu Tyr Ser Gly Ala Lys Ile Thr Ile Ser Tyr 
                565                 570                 575     


Leu Gln Asp Pro Thr Ala Leu Gln Val Asp His Ile Tyr Pro Tyr Ser 
            580                 585                 590         


Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Cys Leu Val Leu Thr Lys 
        595                 600                 605             


Ala Asn Gln Glu Lys Gly Asn Arg Thr Pro Phe Glu Ala Phe Gly Thr 
    610                 615                 620                 


Asp Ser Ala Lys Trp Gly Val Ile Thr Thr Leu Ala Gln Lys Leu Pro 
625                 630                 635                 640 


His Lys Lys Arg Arg Arg Ile Leu Asn Thr Ala Phe Asn Asp Lys Glu 
                645                 650                 655     


Ala Gly Phe Lys Leu Arg Asn Leu Asn Asp Thr Ser Tyr Ile Ala Arg 
            660                 665                 670         


Leu Val Ala Asp Tyr Thr Glu Thr Tyr Leu Glu Phe Leu Pro Leu Val 
        675                 680                 685             


Glu Ser Glu Asn Thr Thr Leu Ser Lys Gly Gln Lys Gly Ser Lys Lys 
    690                 695                 700                 


His Ile Ala Ile Val Asn Gly Val Leu Thr Ser Thr Met Arg Tyr Tyr 
705                 710                 715                 720 


Leu Gly Phe Ala Ser Lys Asp Arg Asp Asn His Leu His His Ala Leu 
                725                 730                 735     


Asp Ala Val Ile Ile Gly Phe Met Asn Asp Ser Val Ile Lys Ala Phe 
            740                 745                 750         


Ser Asp Phe Lys Lys Thr Gln Glu Thr Ser Lys Ala Ala Tyr Tyr Ala 
        755                 760                 765             


His Thr Leu Ser Lys Gln Glu Tyr Lys Lys Gln Arg Ala Phe Ile Gln 
    770                 775                 780                 


Leu Pro Ser Gly Glu Asn Phe Arg Val Ser Val Leu Glu Lys Val Glu 
785                 790                 795                 800 


Ser Val Phe Val Ser Lys Pro Pro Arg Lys Arg Val Arg Gly Ala Leu 
                805                 810                 815     


His Glu Ala Thr Phe Tyr Ser Pro Asn Asp Pro Lys Leu Leu Lys Asn 
            820                 825                 830         


Tyr Gly Gly Thr Lys Gly Val Glu Arg Ala Leu Ala Leu Gly Lys Met 
        835                 840                 845             


Arg Lys Val Gly Ala Lys Ile Val Ser Asn Gly Ala Met Val Arg Val 
    850                 855                 860                 


Asp Ile Phe Arg His Lys Ser Ser Gly Lys Phe Tyr Gly Val Pro Val 
865                 870                 875                 880 


Tyr Thr Met Asp Phe Ala Leu Gly Ile Leu Pro Asn Lys Ala Val Val 
                885                 890                 895     


Val Gly Lys Asp Gln Asn Gly Val Ile Lys Asp Trp Leu Glu Met Asp 
            900                 905                 910         


Ser Ser Tyr Glu Phe Ile Phe Ser Leu Tyr Lys Asp Asp Val Ile Leu 
        915                 920                 925             


Val Gln Lys Lys Glu Met Gln Arg Pro Glu Leu Cys Tyr Phe Val Ser 
    930                 935                 940                 


Phe Asp Thr Ser Gly Ala Ser Ile Lys Val Ala Lys His Asp Asn Asn 
945                 950                 955                 960 


Phe Ser Thr Leu Thr Glu Asn Gln Lys Leu Leu Phe Ser Asn Ala Thr 
                965                 970                 975     


Lys Glu Glu Val Val Gly Lys Ser Ile Gly Ile Gln Asn Leu Lys Val 
            980                 985                 990         


Phe Glu Lys Trp Gln Val Ser Val  Leu Gly Glu Val Lys  Arg Thr Gln 
        995                 1000                 1005             


Ser Tyr  Pro Arg Glu Asn Ile  Ser Leu Ala Ser Lys  Ala Lys Lys 
    1010                 1015                 1020             


<210>  487
<211>  1024
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  487

Met Ile Arg Thr Leu Gly Ile Asp Ile Gly Ile Ala Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Ile Glu Gly Glu Tyr Thr Asp Lys Gly Leu Glu Asn Lys Glu 
            20                  25                  30          


Ile Val Ala Ser Gly Val Arg Val Phe Thr Lys Ala Glu Asn Pro Lys 
        35                  40                  45              


Asn Lys Glu Ser Leu Ala Leu Pro Arg Thr Leu Ala Arg Ser Ala Arg 
    50                  55                  60                  


Arg Arg Asn Ala Arg Lys Lys Gly Arg Ile Gln Gln Val Lys His Tyr 
65                  70                  75                  80  


Leu Ser Lys Ala Leu Gly Leu Asp Leu Glu Cys Phe Val Gln Gly Glu 
                85                  90                  95      


Lys Leu Ala Thr Leu Phe Gln Thr Ser Lys Asp Phe Leu Ser Pro Trp 
            100                 105                 110         


Glu Leu Arg Glu Arg Ala Leu Tyr Arg Val Leu Asp Lys Glu Glu Leu 
        115                 120                 125             


Ala Arg Val Ile Leu His Ile Ala Lys Arg Arg Gly Tyr Asp Asp Ile 
    130                 135                 140                 


Thr Tyr Gly Val Glu Asp Asn Asp Ser Gly Lys Ile Lys Lys Ala Ile 
145                 150                 155                 160 


Ala Glu Asn Ser Lys Arg Ile Lys Glu Glu Gln Cys Lys Thr Ile Gly 
                165                 170                 175     


Glu Met Met Tyr Lys Leu Tyr Phe Gln Lys Ser Leu Asn Val Arg Asn 
            180                 185                 190         


Lys Lys Glu Ser Tyr Asn Arg Cys Val Gly Arg Ser Glu Leu Arg Glu 
        195                 200                 205             


Glu Leu Lys Thr Ile Phe Gln Ile Gln Gln Glu Leu Lys Ser Pro Trp 
    210                 215                 220                 


Val Asn Glu Glu Leu Ile Tyr Lys Leu Leu Gly Asn Pro Asp Ala Gln 
225                 230                 235                 240 


Ser Lys Gln Glu Arg Glu Gly Leu Ile Phe Tyr Gln Arg Pro Leu Lys 
                245                 250                 255     


Gly Phe Gly Asp Lys Ile Gly Lys Cys Ser His Ile Lys Lys Gly Glu 
            260                 265                 270         


Asn Ser Pro Tyr Arg Ala Cys Lys His Ala Pro Ser Ala Glu Glu Phe 
        275                 280                 285             


Val Ala Leu Thr Lys Ser Ile Asn Phe Leu Lys Asn Leu Thr Asn Arg 
    290                 295                 300                 


His Gly Leu Cys Phe Ser Gln Glu Asp Met Cys Val Tyr Leu Gly Lys 
305                 310                 315                 320 


Ile Leu Gln Glu Ala Gln Lys Asn Glu Lys Gly Leu Thr Tyr Ser Lys 
                325                 330                 335     


Leu Lys Leu Leu Leu Asp Leu Pro Ser Asp Phe Glu Phe Leu Gly Leu 
            340                 345                 350         


Asp Tyr Ser Gly Lys Asn Pro Glu Lys Ala Val Phe Leu Ser Leu Pro 
        355                 360                 365             


Ser Thr Phe Lys Leu Asn Lys Ile Thr Gln Asp Arg Lys Thr Gln Asp 
    370                 375                 380                 


Lys Ile Ala Asn Ile Leu Gly Ala Asn Lys Asp Trp Glu Ala Ile Leu 
385                 390                 395                 400 


Lys Glu Leu Glu Ser Leu Gln Leu Ser Lys Glu Gln Ile Gln Thr Ile 
                405                 410                 415     


Lys Asp Ala Lys Leu Asn Phe Ser Lys His Ile Asn Leu Ser Leu Glu 
            420                 425                 430         


Ala Leu Tyr His Leu Leu Pro Leu Met Arg Glu Gly Lys Arg Tyr Asp 
        435                 440                 445             


Glu Gly Val Glu Ile Leu Gln Glu Arg Gly Ile Phe Ser Lys Pro Gln 
    450                 455                 460                 


Pro Lys Asn Arg Gln Leu Leu Pro Pro Leu Ser Glu Leu Ala Lys Glu 
465                 470                 475                 480 


Glu Ser Tyr Phe Asp Ile Pro Asn Pro Val Leu Arg Arg Ala Leu Ser 
                485                 490                 495     


Glu Phe Arg Lys Val Val Asn Ala Leu Leu Glu Lys Tyr Gly Gly Phe 
            500                 505                 510         


His Tyr Phe His Ile Glu Leu Thr Arg Asp Val Cys Lys Ala Lys Ser 
        515                 520                 525             


Ala Arg Met Gln Leu Glu Lys Ile Asn Lys Lys Asn Lys Ser Glu Asn 
    530                 535                 540                 


Asp Ala Ala Ser Gln Leu Leu Glu Val Leu Gly Leu Pro Asn Thr Tyr 
545                 550                 555                 560 


Asn Asn Arg Leu Lys Cys Lys Leu Trp Lys Gln Gln Glu Glu Tyr Cys 
                565                 570                 575     


Leu Tyr Ser Gly Glu Lys Ile Thr Ile Asp His Leu Lys Asp Gln Arg 
            580                 585                 590         


Ala Leu Gln Ile Asp His Ala Phe Pro Leu Ser Arg Ser Leu Asp Asp 
        595                 600                 605             


Ser Gln Ser Asn Lys Val Leu Cys Leu Thr Ser Ser Asn Gln Glu Lys 
    610                 615                 620                 


Ser Asn Lys Thr Pro Tyr Glu Trp Leu Gly Ser Asp Glu Lys Lys Trp 
625                 630                 635                 640 


Asp Met Tyr Val Gly Arg Val Tyr Ser Ser Asn Phe Ser Pro Ser Lys 
                645                 650                 655     


Lys Arg Lys Leu Thr Gln Lys Asn Phe Lys Glu Arg Asn Glu Glu Asp 
            660                 665                 670         


Phe Leu Ala Arg Asn Leu Val Asp Thr Gly Tyr Ile Gly Arg Val Thr 
        675                 680                 685             


Lys Glu Tyr Ile Lys His Ser Leu Ser Phe Leu Pro Leu Pro Asp Gly 
    690                 695                 700                 


Lys Lys Glu His Ile Arg Ile Ile Ser Gly Ser Met Thr Ser Thr Met 
705                 710                 715                 720 


Arg Ser Phe Trp Gly Val Gln Glu Lys Asn Arg Asp His His Leu His 
                725                 730                 735     


His Ala Gln Asp Ala Ile Ile Ile Ala Cys Ile Glu Pro Ser Met Ile 
            740                 745                 750         


Gln Lys Tyr Thr Thr Tyr Leu Lys Asp Lys Glu Thr His Arg Leu Lys 
        755                 760                 765             


Ser His Gln Lys Ala Gln Ile Leu Arg Glu Gly Asp His Lys Leu Ser 
    770                 775                 780                 


Leu Arg Trp Pro Met Ser Asn Phe Lys Asp Lys Ile Gln Glu Ser Ile 
785                 790                 795                 800 


Gln Asn Ile Ile Pro Ser His His Val Ser His Lys Val Thr Gly Glu 
                805                 810                 815     


Leu His Gln Glu Thr Val Arg Thr Lys Glu Phe Tyr Tyr Gln Ala Phe 
            820                 825                 830         


Gly Gly Glu Glu Gly Val Lys Lys Ala Leu Lys Phe Gly Lys Ile Arg 
        835                 840                 845             


Glu Ile Asn Gln Gly Ile Val Asp Asn Gly Ala Met Val Arg Val Asp 
    850                 855                 860                 


Ile Phe Lys Ser Lys Asp Lys Gly Lys Phe Tyr Ala Val Pro Ile Tyr 
865                 870                 875                 880 


Thr Tyr Asp Phe Ala Ile Gly Lys Leu Pro Asn Lys Ala Ile Val Gln 
                885                 890                 895     


Gly Lys Lys Asn Gly Ile Ile Lys Asp Trp Leu Glu Met Asp Glu Asn 
            900                 905                 910         


Tyr Glu Phe Cys Phe Ser Leu Phe Lys Asn Asp Cys Ile Lys Ile Gln 
        915                 920                 925             


Thr Lys Glu Met Gln Glu Ala Val Leu Ala Ile Tyr Lys Ser Thr Asn 
    930                 935                 940                 


Ser Ala Lys Ala Thr Ile Glu Leu Glu His Leu Ser Lys Tyr Ala Leu 
945                 950                 955                 960 


Lys Asn Glu Asp Glu Glu Lys Met Phe Thr Asp Thr Asp Lys Glu Lys 
                965                 970                 975     


Asn Lys Thr Met Thr Arg Glu Ser Cys Gly Ile Gln Gly Leu Lys Val 
            980                 985                 990         


Phe Gln Lys Val Lys Leu Ser Val  Leu Gly Glu Val Leu  Glu His Lys 
        995                 1000                 1005             


Pro Arg  Asn Arg Gln Asn Ile  Ala Leu Lys Thr Thr  Pro Lys His 
    1010                 1015                 1020             


Val 
    


<210>  488
<211>  1025
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  488

Met Gly Gly Tyr Met Pro Gln Leu Met Arg Tyr Arg Leu Ala Leu Asp 
1               5                   10                  15      


Ile Gly Ser Thr Ser Ile Gly Trp Ala Met Ile Arg Leu Ser Met Gln 
            20                  25                  30          


Asn Glu Pro Ile Ala Ile Ile Arg Met Gly Val Arg Ile Phe Ser Asp 
        35                  40                  45              


Gly Arg Asn Pro Lys Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Val 
    50                  55                  60                  


Ala Arg Gln Met Arg Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Thr 
65                  70                  75                  80  


Arg Leu Leu Asn Ala Leu Val Gln Lys Lys Phe Phe Pro Glu Asp Ala 
                85                  90                  95      


Asp Ala Arg Lys Ala Leu Val Val Leu Asn Pro Tyr Glu Leu Arg Lys 
            100                 105                 110         


Glu Gly Leu Tyr Arg Gln Leu Ser Gly Pro Glu Phe Gly Arg Ala Leu 
        115                 120                 125             


Phe His Ile Ser Gln Arg Arg Gly Phe Leu Ser Asn Arg Lys Thr Asp 
    130                 135                 140                 


Lys Lys Asp Asn Asp Ser Ser Val Leu Lys Lys Ala Ile Val Asp Leu 
145                 150                 155                 160 


Arg Gln Gln Leu Glu Val Glu Asn Cys Gln Thr Ile Gly Glu Trp Leu 
                165                 170                 175     


Phe Lys Gln Gln Gln Ser Gly Lys Ser Val Arg Ala Arg Leu Arg Gly 
            180                 185                 190         


Lys Thr Gln Lys Asp Lys Ala Tyr Asp Phe Tyr Ile Asp Arg Ala Met 
        195                 200                 205             


Ile Ala His Glu Phe Asp Thr Leu Trp Val Ser Gln Ala Lys Tyr Asp 
    210                 215                 220                 


Pro Leu Leu Phe Asn Glu Asp Ala Arg Ala Ile Leu Arg Asp Ile Leu 
225                 230                 235                 240 


Leu Tyr Gln Arg Pro Leu Lys Pro Val Lys Pro Gly Arg Cys Thr Leu 
                245                 250                 255     


Ile Pro Asp Glu Ser Arg Ala Pro Leu Ala Leu Pro Leu Thr Gln Gln 
            260                 265                 270         


Ala Arg Ile Tyr Gln Glu Leu Asn His Leu Arg Ile Thr Thr Pro Ala 
        275                 280                 285             


Leu Gly Ala Gln Ala Leu Thr Val Ala Gln Arg Asn Thr Ile Ala Ala 
    290                 295                 300                 


Leu Leu Glu Lys Ser Asp Val Thr Phe Val Lys Ile Ala Lys Ala Leu 
305                 310                 315                 320 


Gly Leu Thr Ser Thr Thr Glu Phe Asn Leu Gln Asp Ile Lys Arg Asp 
                325                 330                 335     


Arg Leu Lys Gly Asn Ala Thr Ala Ile Ile Leu Ala Arg Asn Gln Tyr 
            340                 345                 350         


Phe Gly Arg Arg Trp His Asp Phe Asn Ala Asp Leu Gln Asn Leu Ile 
        355                 360                 365             


Val Asp Lys Leu Val Asn Glu Gly Ser Glu Thr Ala Leu Ile Glu Trp 
    370                 375                 380                 


Leu Lys Ala Asn Thr Asp Val Asp Glu Ser Ala Ala Glu Arg Val Ser 
385                 390                 395                 400 


Gly Val Thr Leu Pro Glu Gly Tyr Gly Ser Leu Ser Val Ala Ala Leu 
                405                 410                 415     


Arg Arg Leu Leu Pro Ala Leu Lys Glu Asp Val Val Val Tyr Ser Glu 
            420                 425                 430         


Ala Val Lys Arg Ala Gly Phe Asp Ser His Ser Ala Leu Ser Leu Ile 
        435                 440                 445             


Glu Gln Thr Gly Glu Leu Tyr Gln Glu Leu Pro Tyr Tyr Gly Glu Val 
    450                 455                 460                 


Leu Gln Arg His Val Gly Phe Gly Ser Gly Asp Pro Asn Asp Ser Pro 
465                 470                 475                 480 


Glu Arg Arg Phe Gly Lys Ile Ala Asn Pro Thr Val His Ile Gly Leu 
                485                 490                 495     


Asn Glu Leu Arg Lys Ile Ile Asn Ala Leu Ile Lys Arg Tyr Gly Asn 
            500                 505                 510         


Pro Ala Glu Val Val Val Glu Leu Ala Arg Asp Leu Lys Leu Ser Arg 
        515                 520                 525             


Glu Lys Arg Lys Glu Ile Gln Glu Glu Gln Lys Gln Arg Gln Asp Arg 
    530                 535                 540                 


Asn Glu Lys Arg Ile Lys Asp Ala Cys Glu Ile Leu Gly Ile Asp Pro 
545                 550                 555                 560 


Leu Val Ile Asp Arg Gly Lys Arg Arg Glu Ile Ser Gln Lys Met Gln 
                565                 570                 575     


Leu Trp Asp Glu Leu Asn Pro Lys Asp Ala Ala Asn Arg Arg Cys Pro 
            580                 585                 590         


Tyr Thr Gly Asp Gln Ile Ser Ile Glu Lys Leu Leu Ser Ala Glu Val 
        595                 600                 605             


Glu Ile Glu His Ile Leu Pro Phe Ser Lys Thr Leu Asp Asp Ser Leu 
    610                 615                 620                 


Asn Asn Lys Thr Val Ala Met Arg Arg Ala Asn Arg Asp Lys Gly Asn 
625                 630                 635                 640 


Arg Thr Pro Tyr Glu Ala Phe Gly Glu Ser Leu Asn Pro Asp Tyr Asp 
                645                 650                 655     


Tyr Asp Ala Ile Leu Gln Arg Ala Ser Leu Met Pro Arg Glu Lys Arg 
            660                 665                 670         


Lys Arg Phe Ala Ser Asp Gly Tyr Gln Gln Trp Leu Arg Asp Asp Lys 
        675                 680                 685             


Asp Phe Leu Ser Arg Ala Leu Asn Asp Thr Ala Tyr Leu Ser Arg Ile 
    690                 695                 700                 


Ala Lys Glu Tyr Leu Thr Leu Ile Cys Pro Pro Asn Ser Val Arg Ala 
705                 710                 715                 720 


Ile Pro Gly Arg Met Thr Ala Gln Leu Arg Ala Met Phe Gly Leu Asn 
                725                 730                 735     


Arg Leu Leu Ser Glu Gly Gly Ile Lys Asn Arg Glu Asp His Arg His 
            740                 745                 750         


His Ala Ile Asp Ala Ala Val Ile Gly Val Thr Asp Gln Gly Leu Leu 
        755                 760                 765             


Gln Lys Phe Ser Arg Ala Ser Ala Arg Thr Arg Glu Leu Gln Leu Ser 
    770                 775                 780                 


Arg Leu Val Asp Asp Met Pro Leu Pro Trp Asp Thr Tyr Arg Glu His 
785                 790                 795                 800 


Val Thr Arg Ala Leu Glu His Leu Val Val Ser His Lys Pro Asp His 
                805                 810                 815     


Gly Tyr Gln Gly Lys Met His Glu Asp Thr Ala Trp Gly Leu Arg Asp 
            820                 825                 830         


Asn Gly Gln Val Thr Arg Arg Val Arg Ser Glu Asp Gly Gly Pro Arg 
        835                 840                 845             


Gln Arg Leu Leu Glu Asn Lys Asn Val Val Lys Ile Asn Ser Thr Arg 
    850                 855                 860                 


Asp Phe Ser Arg His Gly Ile Asp Glu Gln Gly Gln Val Asn Ala Tyr 
865                 870                 875                 880 


Lys Gly Tyr Val Gly Gly Ser Asn Tyr Cys Ile Glu Ile Trp Ile Asp 
                885                 890                 895     


Glu Lys Gly Lys Trp Asn Ser Asp Val Val Ser Thr Phe Gln Ala Tyr 
            900                 905                 910         


Gln Ile Val Arg Glu Leu Gly Glu Lys Ala Ala Trp Gln Lys Leu Arg 
        915                 920                 925             


Asn Pro Leu Leu Ser Leu Ser Ala Lys Pro Leu Val Met Arg Leu Ile 
    930                 935                 940                 


Ile Asp Asp Val Val Lys Met Gln Ile Ala Gly Gln Asn Lys Ile Met 
945                 950                 955                 960 


Arg Val Val Lys Ile Ala Gln Asn Gly Gln Ile Phe Phe Ala Glu His 
                965                 970                 975     


Asn Glu Ala Asn Val Asp Ala Arg Asp Lys Asp Lys Asn Asn Pro Phe 
            980                 985                 990         


Lys Tyr Ile Ser Lys Thr Ala Gly  Ser Leu Gln Lys Ala  Gly Gly Ile 
        995                 1000                 1005             


His Met  Ala Ile Ser Pro Ile  Gly Asp Ala Arg Pro  Val Arg Tyr 
    1010                 1015                 1020             


Gly Arg  
    1025 


<210>  489
<211>  1027
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  489

Met Lys Tyr Thr Leu Gly Leu Asp Val Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Leu Glu Leu Asn Thr Asn Gly Glu Pro Gln Arg Ile Glu Lys 
            20                  25                  30          


Leu Gly Ser Arg Ile Phe Thr Asp Gly Arg His Pro Lys Asp Gly Thr 
        35                  40                  45              


Thr Leu Ala Ser Lys Arg Arg Gln Lys Arg His Glu Arg Arg Arg Arg 
    50                  55                  60                  


Asp Arg Leu His Gln Arg Lys Lys Leu Val Leu Asn Gln Leu Val Ser 
65                  70                  75                  80  


Met Gly Leu Phe Pro Gln Ser Glu Gln Glu Gln Gln Asp Leu Lys Ser 
                85                  90                  95      


Leu Asp Val Leu Thr Leu Arg Ala Asn Gly Val Ser Lys Gln Leu Ser 
            100                 105                 110         


Ala Tyr Glu Leu Gly Arg Val Phe Tyr Tyr Leu Asn Leu Lys Arg Gly 
        115                 120                 125             


Phe Lys Ser Asn Arg Lys Gly Gly Ser Glu Glu Asp Lys Ala Asn Lys 
    130                 135                 140                 


Ile Ser Glu Arg Ile Lys Lys Leu Gln Glu Gln Leu Lys Glu Asn Asp 
145                 150                 155                 160 


Phe Lys Thr Val Gly Gln Tyr Leu His Asp Arg Tyr Ile Lys Gly Leu 
                165                 170                 175     


Ser Thr Lys Ala Thr Ile Glu Asn Asp Phe His Leu Leu Arg Gln Leu 
            180                 185                 190         


Ile Glu Asp Glu Phe Asp Phe Ile Val Glu Thr Gln Ser Lys Phe Phe 
        195                 200                 205             


Pro Glu Ile Ser Asp Glu Gln Trp Asn Thr Leu Arg Asn Arg Met Phe 
    210                 215                 220                 


His Gln Arg Pro Leu Lys Pro Val Glu Thr Gly Thr Cys Ser Leu Phe 
225                 230                 235                 240 


Glu Asp Lys Lys Arg Thr Tyr Lys Tyr Met Pro Ser Phe Glu Glu Tyr 
                245                 250                 255     


Arg Phe Leu Thr Glu Leu Phe Asn Leu Ser Tyr Gln Asp Glu Asn Phe 
            260                 265                 270         


His Ser His Ser Leu Ser Ser Glu Gln Ile Phe Lys Val Phe His Glu 
        275                 280                 285             


Leu Lys Asn Thr Arg Glu Ala Thr Tyr Thr Lys Ile Lys Lys Leu Leu 
    290                 295                 300                 


Lys Leu Pro Ser Asp Leu Ser Phe Ser Ile Glu Arg Ser Lys Ser Lys 
305                 310                 315                 320 


Asp Lys Ile Lys Ile Ser Thr Thr Asn His Phe Phe Ser Lys Ser Leu 
                325                 330                 335     


Leu Phe Ala Glu Glu Trp Ala Ser Leu Asp Leu Lys Leu Lys Asp Glu 
            340                 345                 350         


Val Ala Glu Ile Tyr Phe Ser Asp Met Ser Val Glu Glu Ile Lys Lys 
        355                 360                 365             


Gly Leu Leu Gln Leu Lys Ile Asp Ser Glu Ile Ile Glu Asn Leu Leu 
    370                 375                 380                 


Glu Glu Lys Val Pro Asn Val Gln Glu Ala Thr Cys Ser Tyr Ser Lys 
385                 390                 395                 400 


Glu Ala Leu Gln Lys Ile Val Ser Leu Ile Leu Glu Glu Glu Arg His 
                405                 410                 415     


Pro Thr Leu Ile Val Asp Asp Leu Phe Lys Asn Glu Lys Glu Glu Thr 
            420                 425                 430         


Ser Leu Ile Asp Arg Leu Asp Tyr Tyr Gly Ala Ser Ile Pro Asp Ser 
        435                 440                 445             


Met Gln Pro Ile Pro Gln His Ile Lys Asp Asn Asn Leu Thr Leu Asn 
    450                 455                 460                 


Glu Asp Glu Lys Arg Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ala 
465                 470                 475                 480 


Ala Leu Asn Gln Ile Arg Gln Val Val Asn Asp Leu Ile Glu Gln Tyr 
                485                 490                 495     


Gly Lys Pro Thr Asn Ile His Ile Glu Phe Ala Arg Asp Leu Lys Lys 
            500                 505                 510         


Ser Lys Glu Glu Arg Asp Phe Asp Asn Arg Lys Lys Lys Lys Asn Glu 
        515                 520                 525             


Glu Leu Asn Glu Gln Val Arg Asp Phe Ile Glu Lys His Asn Gln Lys 
    530                 535                 540                 


Pro Ser Ala Phe Asn Phe Glu Arg Val Lys Leu Trp Phe Glu Leu Asp 
545                 550                 555                 560 


Ala Met Asn Asn Gln Val Cys Ala Tyr Ser Gly Lys Thr Ile Ser Ala 
                565                 570                 575     


Arg Met Val Leu Ser Asp Glu Val Gln Val Asp His Ile Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Leu Asp Asp Ser Leu Ala Asn Lys Val Leu Val Leu Ala 
        595                 600                 605             


Ser Glu Asn Val Lys Lys Arg Asn Arg Thr Pro Tyr Glu Ala Phe Gly 
    610                 615                 620                 


Gln Asp Thr Glu Lys Trp Ala Ser Ile Gln Glu Leu Ala Lys Arg Leu 
625                 630                 635                 640 


Pro Tyr His Lys Gln Trp Arg Phe Ala Glu Asp Ala Ile Lys Lys Phe 
                645                 650                 655     


Glu Glu Lys Asp Gln Phe Leu Gln Arg His Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Ile Ser Lys Ile Ala Lys Lys Tyr Leu Gly Ser Ile Thr Asp Pro Tyr 
        675                 680                 685             


Lys Ile Val Ala Ser Arg Gly Gln Met Thr Ser Ile Ile Arg Gly Lys 
    690                 695                 700                 


Leu Gly Leu Asn Gln Phe Ile Gln Lys Pro Asp Gly Thr Lys Asn Arg 
705                 710                 715                 720 


Glu Asp His Arg His His Ala Ile Asp Ala Leu Thr Val Ala Leu Thr 
                725                 730                 735     


Ser Arg Ser Tyr Leu Lys Arg Ile Ser Asp Ala Ser Ala Arg Asn Gln 
            740                 745                 750         


Asp Pro Asn Arg Ile Pro Ile Pro Glu Pro Trp Lys Gly Phe Ile Lys 
        755                 760                 765             


Asp Ala Asp Val Lys Phe Asn Glu Ile Ile Val Ser His Lys Val Asp 
    770                 775                 780                 


His Gly Lys Asn Gly Pro Phe Met Glu Glu Thr Cys Phe Gly Leu Ile 
785                 790                 795                 800 


Lys Asn Pro Asn Lys Tyr Glu Glu Glu Asn Asp Phe Lys Leu Ile Thr 
                805                 810                 815     


Thr Lys Ser Leu Asp Ser Val Lys Asp Pro Ser Thr Ile Arg Ser Gln 
            820                 825                 830         


Ser Leu Gln Asn Leu Ala Glu Lys Ser Gly Val Ile Ser Leu Ala Lys 
        835                 840                 845             


Ser Gly Val Lys Lys Leu Arg Val Tyr Asp Val Thr Lys Glu Asn Pro 
    850                 855                 860                 


Ala Asp Ile Gly Leu Ile Glu Ser Gly Leu Ala Lys Ile Thr His Gly 
865                 870                 875                 880 


Lys Asp Lys Gln His Val Lys His Tyr Gln Lys Gly Asp Ile Asn Tyr 
                885                 890                 895     


Leu Ala Ile Trp Cys Leu Pro Gln Asn Ile Thr Leu Lys Ser Asp Asn 
            900                 905                 910         


His Lys Lys Arg Lys Thr Asp Tyr Ile Phe Thr Ala Ile Lys Thr Phe 
        915                 920                 925             


Asp Leu Asn Ser Lys Asn Pro Asn Asp Leu Arg Pro His Pro Ala Ala 
    930                 935                 940                 


Lys Leu Val Thr Lys Val Tyr Lys Gly Asp Thr Val Ala Leu Glu Val 
945                 950                 955                 960 


Asn Asn Gln Leu Asn Tyr Tyr Ile Val Lys Ser Ile Arg Ala Ala Asn 
                965                 970                 975     


Lys Lys Leu Tyr Phe Leu Lys Ile Asn Gln Ser Ser Glu Leu Ala Asn 
            980                 985                 990         


Glu Thr Gln Phe Ile Pro Ala Tyr  Thr Lys Leu Val Asp  Tyr Lys Phe 
        995                 1000                 1005             


Arg Lys  Val Ser Val Ser Pro  Thr Gly Val Ile Thr  Asp Asn Gly 
    1010                 1015                 1020             


Pro Leu  Leu Lys 
    1025         


<210>  490
<211>  1029
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  490

Met Arg Ser Leu Arg Tyr Arg Leu Ala Leu Asp Leu Gly Ser Thr Ser 
1               5                   10                  15      


Leu Gly Trp Ala Leu Phe Arg Leu Asp Ala Cys Asn Arg Pro Thr Ala 
            20                  25                  30          


Val Ile Lys Ala Gly Val Arg Ile Phe Ser Asp Gly Arg Asn Pro Lys 
        35                  40                  45              


Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Ala Ala Arg Ala Met Arg 
    50                  55                  60                  


Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Thr Arg Met Gln Ala Lys 
65                  70                  75                  80  


Leu Val Glu His Gly Phe Phe Pro Ala Asp Ala Gly Lys Arg Lys Ala 
                85                  90                  95      


Leu Glu Gln Leu Asn Pro Tyr Ala Leu Arg Ala Lys Gly Leu Gln Glu 
            100                 105                 110         


Ala Leu Leu Pro Gly Glu Phe Ala Arg Ala Leu Phe His Ile Asn Gln 
        115                 120                 125             


Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Lys Asp Asn Asp 
    130                 135                 140                 


Ser Gly Val Leu Lys Lys Ala Ile Gly Gln Leu Arg Gln Gln Met Ala 
145                 150                 155                 160 


Glu Gln Gly Ser Arg Thr Val Gly Glu Tyr Leu Trp Thr Arg Leu Gln 
                165                 170                 175     


Gln Gly Gln Gly Val Arg Ala Arg Tyr Arg Glu Lys Pro Tyr Thr Thr 
            180                 185                 190         


Glu Glu Gly Lys Lys Arg Ile Asp Lys Ser Tyr Asp Leu Tyr Ile Asp 
        195                 200                 205             


Arg Ala Met Ile Glu Gln Glu Phe Asp Ala Leu Trp Ala Ala Gln Ala 
    210                 215                 220                 


Ala Phe Asn Pro Thr Leu Phe His Glu Ala Ala Arg Ala Asp Leu Lys 
225                 230                 235                 240 


Asp Thr Leu Leu His Gln Arg Pro Leu Arg Pro Val Lys Pro Gly Arg 
                245                 250                 255     


Cys Thr Leu Leu Pro Glu Glu Glu Arg Ala Pro Leu Ala Leu Pro Ser 
            260                 265                 270         


Thr Gln Arg Phe Arg Ile His Gln Glu Val Asn His Leu Arg Leu Leu 
        275                 280                 285             


Asp Glu Asn Leu Arg Glu Val Ala Leu Thr Leu Ala Gln Arg Asp Ala 
    290                 295                 300                 


Val Val Thr Ala Leu Glu Thr Lys Ala Lys Leu Ser Phe Glu Gln Ile 
305                 310                 315                 320 


Arg Lys Leu Leu Lys Leu Ser Gly Ser Val Gln Phe Asn Leu Glu Asp 
                325                 330                 335     


Ala Lys Arg Thr Glu Leu Lys Gly Asn Ala Thr Ser Ala Ala Leu Ala 
            340                 345                 350         


Arg Lys Glu Leu Phe Gly Ala Ala Trp Ser Gly Phe Asp Glu Ala Leu 
        355                 360                 365             


Gln Asp Glu Ile Val Trp Gln Leu Val Thr Glu Glu Gly Glu Gly Ala 
    370                 375                 380                 


Leu Ile Ala Trp Leu Gln Thr His Thr Gly Val Asp Glu Ala Arg Ala 
385                 390                 395                 400 


Gln Ala Ile Val Asp Val Ser Leu Pro Glu Gly Tyr Gly Asn Leu Ser 
                405                 410                 415     


Arg Lys Ala Leu Ala Arg Ile Val Pro Ala Leu Arg Ala Ala Val Ile 
            420                 425                 430         


Thr Tyr Asp Lys Ala Val Gln Ala Ala Gly Phe Asp His His Ser Gln 
        435                 440                 445             


Leu Gly Phe Glu Tyr Asp Ala Ser Glu Val Glu Asp Leu Val His Pro 
    450                 455                 460                 


Glu Thr Gly Glu Ile Arg Ser Val Phe Lys Gln Leu Pro Tyr Tyr Gly 
465                 470                 475                 480 


Lys Ala Leu Gln Arg His Val Ala Phe Gly Ser Gly Lys Pro Glu Asp 
                485                 490                 495     


Pro Asp Glu Lys Arg Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ile 
            500                 505                 510         


Gly Leu Asn Gln Val Arg Met Val Val Asn Ala Leu Ile Arg Arg Tyr 
        515                 520                 525             


Gly Arg Pro Thr Glu Val Val Ile Glu Leu Ala Arg Asp Leu Lys Gln 
    530                 535                 540                 


Ser Arg Glu Gln Lys Val Glu Ala Gln Arg Arg Gln Ala Asp Asn Gln 
545                 550                 555                 560 


Arg Arg Asn Ala Arg Ile Arg Arg Ser Ile Ala Glu Val Leu Gly Ile 
                565                 570                 575     


Gly Glu Glu Arg Val Arg Gly Ser Asp Ile Gln Lys Trp Ile Cys Trp 
            580                 585                 590         


Glu Glu Leu Ser Phe Asp Ala Ala Asp Arg Arg Cys Pro Tyr Ser Gly 
        595                 600                 605             


Val Gln Ile Ser Ala Ala Met Leu Leu Ser Asp Glu Val Glu Val Glu 
    610                 615                 620                 


His Ile Leu Pro Phe Ser Lys Thr Leu Asp Asp Ser Leu Asn Asn Arg 
625                 630                 635                 640 


Thr Val Ala Met Arg Gln Ala Asn Arg Ile Lys Arg Asn Arg Thr Pro 
                645                 650                 655     


Trp Asp Ala Arg Ala Glu Phe Glu Ala Gln Gly Trp Ser Tyr Glu Asp 
            660                 665                 670         


Ile Leu Gln Arg Ala Glu Arg Met Pro Leu Arg Lys Arg Tyr Arg Phe 
        675                 680                 685             


Ala Pro Asp Gly Tyr Glu Arg Trp Leu Gly Asp Asp Lys Asp Phe Leu 
    690                 695                 700                 


Ala Arg Ala Leu Asn Asp Thr Arg Tyr Leu Ser Arg Val Ala Ala Glu 
705                 710                 715                 720 


Tyr Leu Arg Leu Val Cys Pro Gly Thr Arg Val Ile Pro Gly Gln Leu 
                725                 730                 735     


Thr Ala Leu Leu Arg Gly Lys Phe Gly Leu Asn Asp Val Leu Gly Leu 
            740                 745                 750         


Asp Gly Glu Lys Asn Arg Asn Asp His Arg His His Ala Val Asp Ala 
        755                 760                 765             


Cys Val Ile Gly Val Thr Asp Gln Gly Leu Met Gln Arg Phe Ala Thr 
    770                 775                 780                 


Ala Ser Ala Gln Ala Arg Gly Asp Gly Leu Thr Arg Leu Val Asp Gly 
785                 790                 795                 800 


Met Pro Met Pro Trp Pro Thr Tyr Arg Asp His Val Glu Arg Ala Val 
                805                 810                 815     


Arg His Ile Trp Val Ser His Arg Pro Asp His Gly Phe Glu Gly Ala 
            820                 825                 830         


Met Met Glu Glu Thr Ser Tyr Gly Ile Arg Lys Asp Gly Ser Ile Lys 
        835                 840                 845             


Gln Arg Arg Lys Ala Asp Gly Ser Ala Gly Arg Glu Ile Ser Asn Leu 
    850                 855                 860                 


Ile Arg Ile His Glu Ala Thr Gln Pro Leu Arg His Gly Val Ser Ala 
865                 870                 875                 880 


Asp Gly Gln Pro Leu Ala Tyr Lys Gly Tyr Val Gly Gly Ser Asn Tyr 
                885                 890                 895     


Cys Ile Glu Ile Thr Val Asn Asp Lys Gly Lys Trp Glu Gly Glu Val 
            900                 905                 910         


Ile Ser Thr Phe Arg Ala Tyr Gly Val Val Arg Ala Gly Gly Met Gly 
        915                 920                 925             


Arg Leu Arg Asn Pro His Glu Gly Gln Asn Gly Arg Lys Leu Ile Met 
    930                 935                 940                 


Arg Leu Val Ile Gly Asp Ser Val Arg Leu Glu Val Asp Gly Ala Glu 
945                 950                 955                 960 


Arg Thr Met Arg Ile Val Lys Ile Ser Gly Ser Asn Gly Gln Ile Phe 
                965                 970                 975     


Met Ala Pro Ile His Glu Ala Asn Val Asp Ala Arg Asn Thr Asp Lys 
            980                 985                 990         


Gln Asp Ala Phe Thr Tyr Thr Ser  Lys Tyr Ala Gly Ser  Leu Gln Lys 
        995                 1000                 1005             


Ala Lys  Thr Arg Arg Val Thr  Ile Ser Pro Ile Gly  Glu Val Arg 
    1010                 1015                 1020             


Asp Pro  Gly Phe Lys Gly 
    1025                 


<210>  491
<211>  1029
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  491

Met Lys Tyr Arg Leu Ala Leu Asp Leu Gly Ser Thr Ser Leu Gly Trp 
1               5                   10                  15      


Ala Ile Val Gln Leu Asn Ala Lys Asp Glu Pro Cys Ala Ile Val Arg 
            20                  25                  30          


Ala Gly Val Arg Ile Phe Pro Asp Gly Arg Asn Pro Lys Asp Gly Ser 
        35                  40                  45              


Ser Leu Ala Val Thr Arg Arg Asn Ala Arg Ala Met Arg Arg Arg Arg 
    50                  55                  60                  


Asp Arg Leu Leu Lys Arg Lys Ala Arg Met Gln Arg Leu Leu Ile Glu 
65                  70                  75                  80  


His Asp Leu Phe Pro Ala Asp Glu Ala Ala Arg Lys Ala Leu Glu Thr 
                85                  90                  95      


Leu Asn Pro Tyr Glu Leu Arg Ala Lys Gly Leu Asp Ala Ala Leu Thr 
            100                 105                 110         


Pro Ala Glu Phe Ala Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly 
        115                 120                 125             


Phe Lys Ser Asn Arg Arg Thr Asp Arg Lys Asp Asn Asp Ser Gly Ala 
    130                 135                 140                 


Leu Lys Ala Ala Ile Gly Gly Leu Arg Ala Gln Leu Asp Thr Ala Gly 
145                 150                 155                 160 


Ala Asp Gly Arg Ala Arg Thr Val Gly Glu Leu Leu Trp Arg Arg Met 
                165                 170                 175     


Ser Asp Pro Asn Val Leu Thr Val Arg Ala Arg Tyr Arg Glu Gln Arg 
            180                 185                 190         


Ile Thr Lys Asp Asp Gly Lys Thr Arg Val Asp Lys Ser Tyr Asp Leu 
        195                 200                 205             


Tyr Val Asp Arg Ala Met Val Glu Ala Glu Phe Asp Ala Leu Trp Ala 
    210                 215                 220                 


Lys Gln Ala Glu Leu Ser Pro Thr Gln Phe Gly Glu Ala Ala Arg Ala 
225                 230                 235                 240 


Thr Leu Lys Asp Cys Leu Leu His Gln Arg Asn Leu Arg Pro Val Arg 
                245                 250                 255     


Pro Gly Arg Cys Thr Leu Glu Pro Asp Glu Pro Arg Ala Pro Leu Ala 
            260                 265                 270         


Leu Pro Ser Gln Gln Arg Phe Arg Ile Leu Gln Glu Val Asn His Leu 
        275                 280                 285             


Arg Leu Leu Gly Gly Arg Leu Leu Glu Glu Ser Leu Ser Val Glu Gln 
    290                 295                 300                 


Arg Asp Lys Leu Val Ala Ala Leu Glu Asp Gly Ser Lys Ser Phe Lys 
305                 310                 315                 320 


Thr Ala Ile Pro Lys Leu Leu Gly Leu Pro Gly Thr Val Gln Phe Asn 
                325                 330                 335     


Leu Ala Asp Ala Lys Arg Thr Glu Leu Lys Gly Asn Ala Thr Ser Met 
            340                 345                 350         


Leu Leu Gly Lys Lys Asp Phe Phe Gly Pro Ala Trp His Gly Phe Ser 
        355                 360                 365             


Glu Ala Arg Gln Asp Glu Ile Val Met Arg Ile Gln Glu Glu Glu Ser 
    370                 375                 380                 


Glu Ala Arg Leu Val Asp Trp Leu Ala Thr Ser Thr Gly Val Asp Glu 
385                 390                 395                 400 


Ala His Ala Leu Lys Ile Ala Asn Ala Ala Leu Pro Glu Gly Tyr Gly 
                405                 410                 415     


Ser Leu Ser Ala Lys Ala Leu Ala Lys Ile Leu Pro Ala Met Arg Ala 
            420                 425                 430         


Glu Val Ile Thr Tyr Asp Lys Ala Val Leu Ala Ala Gly Tyr Asp His 
        435                 440                 445             


His Ser His Ile Ser His Ala Ala Ser Thr Gly Glu Ile Leu Pro Glu 
    450                 455                 460                 


Leu Pro Tyr Tyr Gly Glu Tyr Leu Gln Arg His Val Gly Phe Gly Thr 
465                 470                 475                 480 


Gly Glu Leu Asn Asp Thr Pro Glu Lys Arg Phe Gly Arg Ile Ala Asn 
                485                 490                 495     


Pro Thr Val His Ile Gly Leu Asn Gln Val Arg Val Val Val Asn Ala 
            500                 505                 510         


Leu Ile Lys Arg Tyr Gly His Pro Thr Glu Val Ile Val Glu Val Ala 
        515                 520                 525             


Arg Asp Leu Lys Gln Ser Gln Asp Gln Arg Arg Glu Thr Gln Glu Gln 
    530                 535                 540                 


Gln Ala Lys Asn Gln Lys Arg Asn Glu Arg Leu Arg Lys Asp Ala Ala 
545                 550                 555                 560 


Ala Ile Leu Gly Ile Ala Asp Glu Arg Val Arg Leu Ala Asp Ile Gln 
                565                 570                 575     


Lys Leu Ile Leu Trp Glu Glu Leu Ser Phe Asp Ile Ala Asp Arg Arg 
            580                 585                 590         


Cys Pro Tyr Ser Gly Val Gln Leu Ser Ala Ala Met Val Leu Ser Glu 
        595                 600                 605             


Gln Val Glu Ile Glu His Ile Leu Pro Phe Ser Met Thr Leu Asp Asp 
    610                 615                 620                 


Ser Leu Asn Asn Lys Thr Val Ala Met Arg Leu Val Asn Arg Leu Lys 
625                 630                 635                 640 


Gly Asn Arg Thr Pro Trp Asp Ala Arg Glu Ala Phe Ala Gly Gln Gly 
                645                 650                 655     


Trp Asp Tyr Asp Gly Ile Leu Met Arg Ala Glu Arg Met Pro Arg Ala 
            660                 665                 670         


Lys Arg Gln Arg Phe Ala Pro Asp Gly Leu Glu Lys Trp Leu Lys Glu 
        675                 680                 685             


Asp Lys Asp Phe Leu Ala Arg Ala Leu Asn Asp Thr Arg His Leu Ser 
    690                 695                 700                 


Arg Val Ala Arg Glu Tyr Leu Ser Leu Val Cys Pro Ala Ser Thr Arg 
705                 710                 715                 720 


Val Ile Pro Gly Gln Met Thr Ala Met Leu Arg Ala Lys Phe Gly Leu 
                725                 730                 735     


Asn Asp Ile Leu Gly Leu Asn Gly Glu Lys Asn Arg Asn Asp His Arg 
            740                 745                 750         


His His Ala Val Asp Ala Cys Val Val Ala Val Thr Asp Gln Gly Leu 
        755                 760                 765             


Leu Gln Arg Phe Ala Gln Ala Ser Ala Asp Ala Arg Gly Lys Gly Leu 
    770                 775                 780                 


Asp Arg Leu Val Glu Thr Met Pro Asp Pro Trp Asp Ser Tyr Arg Glu 
785                 790                 795                 800 


His Val Gln Arg Ala Val Asn Ser Ile Trp Val Ser His Lys Pro Asp 
                805                 810                 815     


His Gly Phe Glu Gly Ala Met His Glu Asp Thr Thr Tyr Arg Pro Pro 
            820                 825                 830         


His Leu Asp Pro Lys Asn Met Trp Arg Thr Arg Gly Ile Gly Gly Gly 
        835                 840                 845             


Lys Pro Asn Glu Lys Glu Ala Asp Ser Lys Ala Met Val Gly Phe Thr 
    850                 855                 860                 


Gly Pro Glu His Leu Gln Arg His His Arg Asp Lys Ala Gly Gln Gln 
865                 870                 875                 880 


Leu Pro Tyr Lys Gly Tyr Val Ala Ser Pro Lys Phe Cys Met Asp Ile 
                885                 890                 895     


Val Arg Asn Asp Lys Gly Arg Trp Glu Gly Glu Val Val Ser Thr Phe 
            900                 905                 910         


Glu Ala Tyr Arg Leu Val Ser Glu Phe Gly Arg Asp Ala Ala Leu Ala 
        915                 920                 925             


His Ile Gln Ser Lys Ser Arg Ser Ile Ser Gly Lys Pro Leu Val Met 
    930                 935                 940                 


Arg Leu Gln Gly Gly Asp Met Val Arg Phe Asp Asp Asp Glu Gly Arg 
945                 950                 955                 960 


Pro Val Val Leu Arg Val Val Asn Met Thr Gly Asn Gly Gln Ile Phe 
                965                 970                 975     


Phe Ala Pro His Asn Glu Ala Asn Gly Asp Ala Arg Asn Arg Asp Lys 
            980                 985                 990         


Gln Asp Gln Phe Val Tyr Leu Ser  Lys Tyr Ala Gly Ser  Leu Gln Lys 
        995                 1000                 1005             


Ala Arg  Gly Arg Arg Val Thr  Val Ser Pro Ala Gly  Glu Leu Arg 
    1010                 1015                 1020             


Asp Pro  Gly Phe Lys Glu 
    1025                 


<210>  492
<211>  1029
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  492

Met Arg Ser Leu Arg Tyr Arg Leu Ala Leu Asp Leu Gly Ser Thr Ser 
1               5                   10                  15      


Leu Gly Trp Ala Leu Phe Arg Leu Asp Ala Cys Asn Arg Pro Thr Ala 
            20                  25                  30          


Val Ile Lys Ala Gly Val Arg Ile Phe Ser Asp Gly Arg Asn Pro Lys 
        35                  40                  45              


Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Ala Ala Arg Ala Met Arg 
    50                  55                  60                  


Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Thr Arg Met Gln Ala Lys 
65                  70                  75                  80  


Leu Val Glu His Gly Phe Phe Pro Ala Asp Ala Gly Lys Arg Lys Ala 
                85                  90                  95      


Leu Glu Gln Leu Asn Pro Tyr Ala Leu Arg Ala Lys Gly Leu Gln Glu 
            100                 105                 110         


Ala Leu Leu Pro Gly Glu Phe Ala Arg Ala Leu Phe His Ile Asn Gln 
        115                 120                 125             


Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Lys Asp Asn Asp 
    130                 135                 140                 


Ser Gly Ala Leu Lys Lys Ala Ile Gly Gln Leu Arg Gln Gln Met Ala 
145                 150                 155                 160 


Glu Gln Gly Ser Arg Thr Val Gly Glu Tyr Leu Trp Thr Arg Leu Gln 
                165                 170                 175     


Gln Gly Gln Gly Val Arg Ala Arg Tyr Arg Glu Lys Pro Tyr Thr Thr 
            180                 185                 190         


Glu Glu Gly Lys Lys Arg Ile Asp Lys Ser Tyr Asp Leu Tyr Ile Asp 
        195                 200                 205             


Arg Ala Met Ile Glu Gln Glu Phe Asp Ala Leu Trp Ala Ala Gln Ala 
    210                 215                 220                 


Ala Phe Asn Pro Thr Leu Phe His Glu Ala Ala Arg Ala Asp Leu Lys 
225                 230                 235                 240 


Asp Thr Leu Leu His Gln Arg Pro Leu Arg Pro Val Lys Pro Gly Arg 
                245                 250                 255     


Cys Thr Leu Leu Pro Glu Glu Glu Arg Ala Pro Leu Ala Leu Pro Ser 
            260                 265                 270         


Thr Gln Arg Phe Arg Ile His Gln Glu Val Asn His Leu Arg Leu Leu 
        275                 280                 285             


Asp Glu Asn Leu Arg Glu Val Ala Leu Thr Leu Ala Gln Arg Asp Ala 
    290                 295                 300                 


Val Val Thr Ala Leu Glu Thr Lys Ala Lys Leu Ser Phe Glu Gln Ile 
305                 310                 315                 320 


Arg Lys Leu Leu Lys Leu Ser Gly Ser Val Gln Phe Asn Leu Glu Asp 
                325                 330                 335     


Ala Lys Arg Thr Glu Leu Lys Gly Asn Ala Thr Ser Ala Ala Leu Ala 
            340                 345                 350         


Arg Lys Glu Leu Phe Gly Ala Ala Trp Ser Gly Phe Asp Glu Ala Leu 
        355                 360                 365             


Gln Asp Glu Ile Val Trp Gln Leu Val Thr Glu Glu Gly Glu Gly Ala 
    370                 375                 380                 


Leu Ile Ala Trp Leu Gln Thr His Thr Gly Val Asp Glu Ala Arg Ala 
385                 390                 395                 400 


Gln Ala Ile Val Asp Val Ser Leu Pro Glu Gly Tyr Gly Asn Leu Ser 
                405                 410                 415     


Arg Lys Ala Leu Ala Arg Ile Val Pro Ala Leu Arg Ala Ala Val Ile 
            420                 425                 430         


Thr Tyr Asp Lys Ala Val Gln Ala Ala Gly Phe Asp His His Ser Gln 
        435                 440                 445             


Leu Gly Phe Glu Tyr Asp Ala Ser Glu Val Glu Asp Leu Val His Pro 
    450                 455                 460                 


Glu Thr Gly Glu Ile Arg Ser Val Phe Lys Gln Leu Pro Tyr Tyr Gly 
465                 470                 475                 480 


Lys Ala Leu Gln Arg His Val Ala Phe Gly Ser Gly Lys Pro Glu Asp 
                485                 490                 495     


Pro Asp Glu Lys Arg Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ile 
            500                 505                 510         


Gly Leu Asn Gln Val Arg Met Val Val Asn Ala Leu Ile Arg Arg Tyr 
        515                 520                 525             


Gly Arg Pro Thr Glu Val Val Ile Glu Leu Ala Arg Asp Leu Lys Gln 
    530                 535                 540                 


Ser Arg Glu Gln Lys Val Glu Ala Gln Arg Arg Gln Ala Asp Asn Gln 
545                 550                 555                 560 


Arg Arg Asn Ala Arg Ile Arg Arg Ser Ile Ala Glu Val Leu Gly Ile 
                565                 570                 575     


Gly Glu Glu Arg Val Arg Gly Ser Asp Ile Gln Lys Trp Ile Cys Trp 
            580                 585                 590         


Glu Glu Leu Ser Phe Asp Ala Ala Asp Arg Arg Cys Pro Tyr Ser Gly 
        595                 600                 605             


Val Gln Ile Ser Ala Ala Met Leu Leu Ser Asp Glu Val Glu Val Glu 
    610                 615                 620                 


His Ile Leu Pro Phe Ser Lys Thr Leu Asp Asp Ser Leu Asn Asn Arg 
625                 630                 635                 640 


Thr Val Ala Met Arg Gln Ala Asn Arg Ile Lys Arg Asn Arg Thr Pro 
                645                 650                 655     


Trp Asp Ala Arg Ala Glu Phe Glu Ala Gln Gly Trp Ser Tyr Glu Asp 
            660                 665                 670         


Ile Leu Gln Arg Ala Glu Arg Met Pro Leu Arg Lys Arg Tyr Arg Phe 
        675                 680                 685             


Ala Pro Asp Gly Tyr Glu Arg Trp Leu Gly Asp Asp Lys Asp Phe Leu 
    690                 695                 700                 


Ala Arg Ala Leu Asn Asp Thr Arg Tyr Leu Ser Arg Val Ala Ala Glu 
705                 710                 715                 720 


Tyr Leu Arg Leu Val Cys Pro Gly Thr Arg Val Ile Pro Gly Gln Leu 
                725                 730                 735     


Thr Ala Leu Leu Arg Gly Lys Phe Gly Leu Asn Asp Val Leu Gly Leu 
            740                 745                 750         


Asp Gly Glu Lys Asn Arg Asn Asp His Arg His His Ala Val Asp Ala 
        755                 760                 765             


Cys Val Ile Gly Val Thr Asp Gln Gly Leu Met Gln Arg Phe Ala Thr 
    770                 775                 780                 


Ala Ser Ala Gln Ala Arg Gly Asp Gly Leu Thr Arg Leu Val Asp Gly 
785                 790                 795                 800 


Met Pro Met Pro Trp Pro Thr Tyr Arg Asp His Val Glu Arg Ala Val 
                805                 810                 815     


Arg His Ile Trp Val Ser His Arg Pro Asp His Gly Phe Glu Gly Ala 
            820                 825                 830         


Met Met Glu Glu Thr Ser Tyr Gly Ile Arg Lys Asp Gly Ser Ile Lys 
        835                 840                 845             


Gln Arg Arg Lys Ala Asp Gly Ser Ala Gly Arg Glu Ile Ser Asn Leu 
    850                 855                 860                 


Ile Arg Ile His Glu Ala Thr Gln Pro Leu Arg His Gly Val Ser Ala 
865                 870                 875                 880 


Asp Gly Gln Pro Leu Ala Tyr Lys Gly Tyr Val Gly Gly Ser Asn Tyr 
                885                 890                 895     


Cys Ile Glu Ile Thr Val Asn Asp Lys Gly Lys Trp Glu Gly Glu Val 
            900                 905                 910         


Ile Ser Thr Phe Arg Ala Tyr Gly Ile Val Arg Ala Gly Gly Met Gly 
        915                 920                 925             


Arg Leu Arg Asn Pro His Glu Gly Gln Asn Gly Arg Lys Leu Ile Met 
    930                 935                 940                 


Arg Leu Val Ile Gly Asp Ser Val Arg Leu Glu Val Asp Gly Ala Glu 
945                 950                 955                 960 


Arg Thr Met Arg Ile Val Lys Ile Ser Gly Ser Asn Gly Gln Ile Phe 
                965                 970                 975     


Met Ala Pro Ile His Glu Ala Asn Val Asp Ala Arg Asn Thr Asp Lys 
            980                 985                 990         


Gln Asp Ala Phe Thr Tyr Thr Ser  Lys Tyr Ala Gly Ser  Leu Gln Lys 
        995                 1000                 1005             


Ala Lys  Thr Arg Arg Val Thr  Ile Ser Pro Ile Gly  Glu Val Arg 
    1010                 1015                 1020             


Asp Pro  Gly Phe Lys Gly 
    1025                 


<210>  493
<211>  1031
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  493

Met Pro Ala Ile Tyr Arg Leu Ala Leu Asp Leu Gly Ser Thr Ser Leu 
1               5                   10                  15      


Gly Trp Ala Val Leu Arg Leu Glu Pro Ala Ala Leu Glu Pro Ile Ala 
            20                  25                  30          


Ile Ile Lys Ala Gly Val Arg Ile Phe Ser Asp Gly Arg Asn Pro Lys 
        35                  40                  45              


Asp Gly Ala Ser Leu Ala Val Thr Arg Arg Ala Ala Arg Ala Met Arg 
    50                  55                  60                  


Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Ala Arg Met Gln Asp Lys 
65                  70                  75                  80  


Leu Val Lys His Gly Phe Phe Pro Ser Asp Met Asp Glu Arg Lys Ala 
                85                  90                  95      


Leu Glu Arg Leu Asp Pro Tyr Ala Leu Arg Ala Lys Gly Leu Arg Glu 
            100                 105                 110         


Ala Leu Thr Pro Gly Glu Phe Ala Arg Ala Leu Phe His Ile Asn Gln 
        115                 120                 125             


Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Lys Asp Asn Asp 
    130                 135                 140                 


Ser Gly Ala Leu Lys Lys Ala Ile Ser His Leu Arg Gln Gln Met Glu 
145                 150                 155                 160 


Ala Gly Gly His Arg Thr Val Gly Asp His Leu Trp Ser Arg Leu Gln 
                165                 170                 175     


Gln Gly Leu Gly Val Arg Ala Arg Tyr Arg Glu Thr Pro Phe Thr Asn 
            180                 185                 190         


Asp Glu Gly Lys Lys Arg Ile Asp Lys Ser Tyr Asp Leu Tyr Ile Asp 
        195                 200                 205             


Arg Ala Met Ile Glu Gln Glu Phe Asp Ala Leu Trp Ala Ala Gln Ala 
    210                 215                 220                 


Ala Phe Asn Pro Ala Leu Phe His Glu Val Ala Arg Ala Asp Leu Lys 
225                 230                 235                 240 


Asp Thr Leu Leu His Gln Arg Arg Leu Arg Pro Val Lys Pro Gly Arg 
                245                 250                 255     


Cys Thr Leu Leu Pro Glu Glu Glu Arg Ala Pro Leu Ala Leu Pro Ser 
            260                 265                 270         


Thr Gln Arg Phe Arg Ile Tyr Gln Glu Val Asn His Leu Arg Leu Leu 
        275                 280                 285             


Gly Glu Asp Leu Arg Glu Val Ala Leu Thr Leu Ala Gln Arg Asp Ala 
    290                 295                 300                 


Val Ala Asn Ala Leu Glu Ser Lys Ala Arg Leu Thr Phe Glu Gln Ile 
305                 310                 315                 320 


Arg Lys Leu Leu Lys Leu Ser Gly Ser Val Gln Phe Asn Leu Glu Asp 
                325                 330                 335     


Ala Lys Arg Thr Glu Leu Lys Gly Asn Ala Thr Ser Ala Ala Leu Ala 
            340                 345                 350         


Arg Lys Glu Leu Phe Gly Ala Ala Trp Gly Gly Phe Gly Glu Ala Leu 
        355                 360                 365             


Gln Asp Glu Ile Val Trp Gln Leu Val Thr Glu Glu Gly Glu Gly Ala 
    370                 375                 380                 


Leu Ile Ala Trp Leu Gln Ala His Thr Gly Val Asp Glu Ala Arg Ala 
385                 390                 395                 400 


Gln Ala Ile Ala Asp Ala Ser Leu Pro Glu Gly Tyr Gly Ser Leu Ser 
                405                 410                 415     


Arg Lys Ala Leu Ala Arg Ile Val Pro Ala Leu Arg Ala Gly Val Val 
            420                 425                 430         


Thr Tyr Asp Lys Ala Val Gln Ala Ala Gly Phe Asp His His Ser Gln 
        435                 440                 445             


Leu Gly Phe Glu Tyr Asp Ala Gly Glu Val Glu Asp Trp Val His Pro 
    450                 455                 460                 


Asp Thr Gly Glu Ile Arg Pro Val Phe Lys Gln Leu Pro Tyr Tyr Gly 
465                 470                 475                 480 


Lys Pro Leu Gln Arg His Val Ala Phe Gly Ser Gly Lys Pro Glu Asp 
                485                 490                 495     


Pro Asp Glu Arg Arg Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ile 
            500                 505                 510         


Gly Leu Asn Gln Val Arg Thr Val Val Asn Ala Leu Ile Arg Arg Tyr 
        515                 520                 525             


Gly Arg Pro Thr Glu Val Val Ile Glu Leu Ala Arg Asp Leu Lys Gln 
    530                 535                 540                 


Ser Arg Glu Gln Lys Val Glu Ala Gln Arg Lys Gln Ala Glu Asn Gln 
545                 550                 555                 560 


Arg Arg Asn Ala Arg Ile Arg Lys Ser Ile Ala Glu Val Leu Gly Ile 
                565                 570                 575     


Gly Glu Glu Gln Val Arg Gly Ala Asp Ile Gln Lys Trp Ile Cys Trp 
            580                 585                 590         


Glu Glu Leu Ser Phe Asp Val Ala Glu Arg Arg Cys Pro Tyr Ser Gly 
        595                 600                 605             


Val Gln Ile Ser Ala Arg Met Leu Leu Ser Asp Glu Val Glu Val Glu 
    610                 615                 620                 


His Ile Leu Pro Phe Ser Lys Thr Leu Asp Asp Ser Leu Asn Asn Arg 
625                 630                 635                 640 


Thr Val Ala Met Arg Gln Ala Asn Arg Ile Lys Arg Asn Arg Thr Pro 
                645                 650                 655     


Trp Thr Ala Arg Asp Asp Phe Glu Ala Gln Gly Trp Ser Tyr Glu Gly 
            660                 665                 670         


Ile Leu Gln Arg Ala Glu Arg Met Pro Leu Arg Lys Arg Tyr Arg Phe 
        675                 680                 685             


Ala Pro Asp Gly Tyr Glu Arg Trp Leu Gly Asp Asp Lys Asp Phe Leu 
    690                 695                 700                 


Ala Arg Ala Leu Asn Asp Thr Arg Tyr Leu Ser Arg Val Ala Ala Glu 
705                 710                 715                 720 


Tyr Leu Arg Leu Val Cys Pro Gly Ser Gly Thr Arg Val Ile Pro Gly 
                725                 730                 735     


Gln Met Thr Ala Arg Leu Arg Gly Lys Phe Gly Leu Asn Gly Val Leu 
            740                 745                 750         


Gly Pro Asp Gly Glu Lys Asn Arg Asn Asp His Arg His His Ala Val 
        755                 760                 765             


Asp Ala Cys Val Ile Gly Val Thr Asp Gln Gly Leu Met Gln Arg Phe 
    770                 775                 780                 


Ala Asn Ala Ser Ala Gln Ala Arg Glu Asp Gly Leu Thr Arg Leu Val 
785                 790                 795                 800 


Asp Asp Met Pro Arg Pro Trp Pro Thr Tyr Arg Ala His Val Glu Arg 
                805                 810                 815     


Ala Val Arg His Ile Trp Val Ser His Arg Pro Asp His Gly Phe Glu 
            820                 825                 830         


Gly Ala Met Met Glu Glu Thr Ser Tyr Gly Ile Gly Lys Asp Gly Ser 
        835                 840                 845             


Ile Lys Gln Arg Arg Lys Ala Asp Gly Ser Pro Gly Arg Glu Ile Thr 
    850                 855                 860                 


Asn Leu Ile Arg Ile His Glu Ala Thr Gln Pro Gln Arg His Gly Ala 
865                 870                 875                 880 


Gly Pro Asp Gly Gln Pro Leu Pro Tyr Lys Gly Tyr Val Gly Gly Ser 
                885                 890                 895     


Asn Tyr Cys Ile Glu Ile Thr Arg Asn Asp Lys Gly Lys Trp Glu Gly 
            900                 905                 910         


Glu Val Ile Ser Thr Phe Arg Ala Tyr Gly Ile Val Arg Thr Ser Gly 
        915                 920                 925             


Leu Gly Gln Leu Arg His Pro Arg Val Gly Gln Asn Gly Arg Glu Leu 
    930                 935                 940                 


Val Met Arg Leu Val Ile Gly Asp Ser Val Arg Leu Glu Ile Asp Gly 
945                 950                 955                 960 


Ile Glu Arg Thr Met Arg Ile Val Lys Ile Ser Gly Ser Asn Gly Gln 
                965                 970                 975     


Ile Phe Met Ala Pro Ile His Glu Ala Asn Val Asp Ala Arg Asn Ser 
            980                 985                 990         


Asp Lys Gln Asp Ala Phe Ala Tyr  Thr Ser Lys Tyr Ala  Gly Ser Leu 
        995                 1000                 1005             


Gln Lys  Thr Lys Ala Arg Phe  Val Thr Ile Ser Pro  Ile Gly Glu 
    1010                 1015                 1020             


Val Arg  Asp Leu Gly Phe Lys  Gly 
    1025                 1030     


<210>  494
<211>  1032
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  494

Met Lys Lys Arg Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Trp Ala Leu Val Glu Phe Asp Asp Glu Phe Gln Asp Asn Lys Lys Lys 
            20                  25                  30          


Gly Thr Ile Ile Lys Ser Gly Val Arg Ile Phe Thr Arg Ala Glu Thr 
        35                  40                  45              


Pro Lys Glu Arg Glu Ser Leu Ala Lys Pro Arg Arg Glu Ala Arg Ser 
    50                  55                  60                  


Gln Arg Arg Arg Ile Arg Arg Arg Val Glu Arg Leu Asn Ser Ile Arg 
65                  70                  75                  80  


Glu Leu Phe Val Lys Asn Gly Val Val Glu Glu Trp Ser Val Ser Lys 
                85                  90                  95      


Lys Asn Pro Asn Asn Ile Tyr Ile Thr Leu Asn Gln Ile Lys Thr Pro 
            100                 105                 110         


Trp Gln Leu Arg Met Glu Ser Leu Asp Arg Lys Leu Thr Asn Glu Gln 
        115                 120                 125             


Phe Ala Val Val Leu Thr His Leu Ala Lys His Arg Gly Tyr Lys Ser 
    130                 135                 140                 


Leu Arg Lys Asp Asp Lys Ala Gly Lys Val Leu Glu Ala Ile Ser Gln 
145                 150                 155                 160 


Asn Lys Lys Arg Leu Asn Asp Ser Asp Tyr Arg Thr Leu Gly Glu Met 
                165                 170                 175     


Phe Cys Lys Asn Glu Ser Tyr Lys Glu His Lys Arg Asn Lys Gly Gly 
            180                 185                 190         


Glu Tyr Ile Asn Ser Val Ala Arg Glu Leu Ile Glu Glu Glu Val Glu 
        195                 200                 205             


Lys Ile Phe Val Lys Gln Arg Glu Lys Gly Asn Asn Phe Ala Asp Val 
    210                 215                 220                 


Asn Thr Glu Arg Glu Tyr Leu Lys Ile Ala Phe Ser Gln Lys Pro Ala 
225                 230                 235                 240 


Gly Ser Ile Ser Lys Met Val Gly Phe Cys Thr Phe Glu Asn Gly Glu 
                245                 250                 255     


Lys Arg Ala Pro Arg Asp Ser Tyr Ser Ala Glu Leu Phe Lys Val Leu 
            260                 265                 270         


Cys Thr Ile Asn Asn Thr Thr Phe Thr Asp Met Lys Thr Gly Glu Ile 
        275                 280                 285             


Arg Ser Phe Ser Lys Glu Glu Ile Gly Lys Thr Leu Glu Thr Val Lys 
    290                 295                 300                 


Asn Val Lys Asp Val Lys Tyr Ala Lys Ile Arg Lys Asp Leu Gly Leu 
305                 310                 315                 320 


Glu Asp Ser Val Lys Phe Ser Gly Val Asp Tyr Arg Glu Asn Ala Lys 
                325                 330                 335     


Ser Lys Gly Glu Gly Glu Ile Phe Val Ser Met Lys Thr Tyr His Glu 
            340                 345                 350         


Ile Lys Ser Lys Ile Glu Lys Leu Ser Lys Glu Lys Trp Asp Asn Leu 
        355                 360                 365             


Lys Gly Asn Thr Glu Leu Leu Asp Ser Ile Val Asn Ile Ile Ala Asn 
    370                 375                 380                 


Leu Lys Asp Asp Glu Ser Ile Lys Lys Glu Phe Gln Lys Leu Lys Ile 
385                 390                 395                 400 


Asp Glu Asp Ile Ile Asp Val Leu Leu Asn Phe Glu Phe Lys Lys Phe 
                405                 410                 415     


Ile Asn Leu Ser Phe Lys Ala Leu Arg Lys Ile Ile Pro Phe Gln Phe 
            420                 425                 430         


Gln Gly Gln Lys Tyr Ile Thr Ala Ser Thr Ser Ala Gly Tyr Ser Leu 
        435                 440                 445             


Lys Gln Glu Gly Ile Lys Asn Lys Leu Val Pro Pro Ala Lys Glu Asn 
    450                 455                 460                 


Ile Asn Val Ala Val Val Ala Arg Ala Phe Ala Gln Thr Arg Lys Val 
465                 470                 475                 480 


Ile Asn Ala Val Ile Arg Lys Tyr Gly Gln Phe Asp Gln Ile Asn Ile 
                485                 490                 495     


Glu Leu Ala Thr Glu Leu Lys Asn Ser Lys Phe Asp Arg Ser Lys Ile 
            500                 505                 510         


Glu Glu Gly Gln Lys Lys Phe Gln Ser Gln Lys Asp Lys Ile Phe Lys 
        515                 520                 525             


Glu Val Gln Glu Leu Arg Gly Gly Met Thr Pro Ser Ser Ser Leu Leu 
    530                 535                 540                 


Leu Lys Tyr Arg Leu Trp Arg Glu Gln Asp Glu Arg Cys Val Tyr Ser 
545                 550                 555                 560 


Gly Arg Lys Ile Ser Phe Glu Asp Leu Leu Asp Thr Gly Phe Leu Asp 
                565                 570                 575     


Ile Asp His Ile Ile Pro Tyr Ser Lys Ser Met Asp Asp Ser Phe Asn 
            580                 585                 590         


Asn Lys Val Leu Cys Leu Ala Glu Glu Asn Arg Asn Lys Lys Asn Asp 
        595                 600                 605             


Ile Pro Phe Asn Tyr Tyr Gln Arg Ile Asp Arg Asp Trp Asn Leu Leu 
    610                 615                 620                 


Ile Ser Ile Ile Thr Ser Met Lys Asn Met Lys Ile Ala Lys Lys Ser 
625                 630                 635                 640 


Arg Leu Leu Lys Gln Glu Leu Ser Asp Ile Glu Gly Phe Ile Glu Arg 
                645                 650                 655     


Asn Ile Asn Asp Thr Arg Tyr Ala Thr Arg Tyr Ile Lys Asp Phe Leu 
            660                 665                 670         


Glu Asn Asn Leu Glu Phe Lys Glu Asn Glu Phe Ile Lys Val Lys Val 
        675                 680                 685             


Gln Ala Arg Ser Gly Gly Leu Thr Ser Val Leu Ser Tyr Asn Trp Gly 
    690                 695                 700                 


Met Ser Lys Asp Arg Glu Glu Ser Tyr Phe His His Ala Lys Asp Ala 
705                 710                 715                 720 


Ile Ile Val Ala Cys Ser Thr Gln Gly Met Val Gln Tyr Ile Ser Lys 
                725                 730                 735     


Ile Ser Gln Gln Tyr Glu Asn Asp Lys Asp Lys Met Lys Lys Ile Lys 
            740                 745                 750         


Asp Lys Thr Glu Asn Cys Ile Pro Lys Pro Trp Asp Ser Phe Arg Ala 
        755                 760                 765             


Asp Val Glu Glu Ser Ile Glu Gly Ile Phe Val Ser Phe Ala Pro Arg 
    770                 775                 780                 


His Lys Val Thr Gly Ser Ala His Lys Glu Thr Ile Tyr Ser Lys Lys 
785                 790                 795                 800 


His Leu Glu Lys Glu Arg Tyr Val Thr Val Lys Lys Ser Leu Asp Lys 
                805                 810                 815     


Ile Lys Leu Ser Asp Leu Glu Asn Ile Pro Cys Asn Glu Asn Cys Gly 
            820                 825                 830         


Ile Ile Arg Val Leu Lys Glu Arg Leu Glu Lys Phe Lys Asn Asp Ser 
        835                 840                 845             


Leu Lys Ala Phe Ala Asp Pro Val His Met Pro Thr Lys Cys Pro Asn 
    850                 855                 860                 


Lys Lys Gly Pro Val Ile Arg Ser Val Lys Ile Lys Glu Ser Asn Lys 
865                 870                 875                 880 


Thr Gly Ile Glu Val Arg Lys Gly Leu Ala Glu Arg Gly Glu Met Val 
                885                 890                 895     


Arg Val Asp Val Phe Ile Lys Asp Lys Lys Tyr Tyr Leu Val Pro Ile 
            900                 905                 910         


Tyr Val Ser Asp Phe Lys Asn Lys Asp Leu Pro Asn Lys Ala Ile Ala 
        915                 920                 925             


Ala Leu Lys Leu Glu Asn Glu Trp Ile Met Ile Asp Lys Thr Tyr Ser 
    930                 935                 940                 


Phe Lys Phe Ser Leu Phe Lys Asp Asp Leu Ile Lys Ile Lys Lys Lys 
945                 950                 955                 960 


Lys Glu Glu Ile Phe Gly Tyr Phe Gly Gly Val His Arg Ser Thr Gly 
                965                 970                 975     


Thr Ile Lys Ile Lys Ser Arg His Lys Pro Lys Glu Ile Asp Glu Gly 
            980                 985                 990         


Ile Gly Ser Arg Cys Leu Leu Asp  Phe Arg Lys Phe Gln  Val Asp Val 
        995                 1000                 1005             


Leu Gly  Asn Tyr Thr Glu Val  Lys His Glu Lys Arg  Met Pro Ala 
    1010                 1015                 1020             


Tyr Ile  Thr Arg Lys Asp Lys  Lys His 
    1025                 1030         


<210>  495
<211>  1034
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  495

Met Lys Ile Leu Gly Phe Asp Ile Gly Ile Ala Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Asn Gly Glu Leu Arg Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Thr Gly Asp Ser Leu Ala Met Pro Arg Arg 
        35                  40                  45              


Glu Ala Arg Ser Val Arg Arg Arg Leu Ala Arg Arg Lys Gly Arg Leu 
    50                  55                  60                  


Glu Thr Leu Lys Arg Leu Leu Ala Lys Glu Trp Asp Leu Cys Tyr Glu 
65                  70                  75                  80  


Asp Tyr Ile Ala Ala Asp Gly Glu Leu Pro Lys Ala Phe Met Gly Lys 
                85                  90                  95      


Asn Leu Thr Asn Pro Tyr Val Leu Arg Tyr Glu Ala Leu Gln Arg Leu 
            100                 105                 110         


Leu Gly Lys Glu Glu Leu Ala Arg Val Ile Leu His Ile Ala Lys His 
        115                 120                 125             


Arg Gly Tyr Gly Asn Lys Asn Ala Lys Ile Thr Lys Ser Glu Glu Ser 
    130                 135                 140                 


Lys Arg Glu Gln Gly Lys Ile Leu Ser Ala Leu Ala Thr Asn Ala Ser 
145                 150                 155                 160 


Val Ile Ala Arg Tyr Arg Thr Val Gly Glu Tyr Phe Tyr Lys Glu Phe 
                165                 170                 175     


Cys Glu Val Ile Lys Asn Pro Gln Gly Leu Asn Thr Asn Glu Asn Cys 
            180                 185                 190         


Thr Gln Pro Lys Val Arg Val Leu Lys Pro Ile Arg Asn Lys Gly Gly 
        195                 200                 205             


Glu Tyr Thr Asn Cys Ile Leu Gln Glu Asp Leu Gln Arg Glu Leu Arg 
    210                 215                 220                 


Cys Ile Phe Glu His Gln Lys Gly Phe Gly Phe Pro Ile Thr Gln Glu 
225                 230                 235                 240 


Phe Gln Asp Lys Ile Leu Lys Ile Ala Phe Tyr Gln Arg Ser Leu Lys 
                245                 250                 255     


Asp Phe Ser His Leu Val Gly Lys Cys Thr Phe Tyr Pro Asp Glu Pro 
            260                 265                 270         


Arg Ala Pro Lys Phe Ser Leu Ser Ala Ile Glu Phe Ile Thr Lys Ala 
        275                 280                 285             


Lys Thr Ile Asn Leu Leu Val Gly Ile Val Lys Glu Ser Gly Glu Val 
    290                 295                 300                 


Trp Asp Lys Glu Gln Trp Arg Glu Arg Leu Asp Ser Val Phe Ser Ala 
305                 310                 315                 320 


Val Cys Glu Arg Gly Asn Leu Thr Phe Ala Gln Met Arg Lys Ile Leu 
                325                 330                 335     


Lys Ile Asp Glu Arg Ile Gln Phe Lys Glu Val Asp Tyr Thr Ala Lys 
            340                 345                 350         


Asn Pro Glu Asn Lys Lys Phe Val Glu Phe Lys Asn Leu Lys Lys Phe 
        355                 360                 365             


Cys Glu Ile Leu Gly Ala Ile Val Gly Asp Arg Ala Leu Ala Asp Ser 
    370                 375                 380                 


Ile Ala Arg Asp Met Thr Leu Ile Lys Asp Glu Glu Glu Leu Ala Gln 
385                 390                 395                 400 


Lys Leu Arg Gly Tyr Gly Lys Phe Ser Gln Glu Gln Ile Thr Gln Leu 
                405                 410                 415     


Gly Ala Leu Asn Phe Ser His His Ile Asn Leu Ser Leu Lys Ala Leu 
            420                 425                 430         


Ser Glu Ile Leu Pro Phe Met Arg Glu Gly Met Arg Tyr Asp Glu Ala 
        435                 440                 445             


Cys Gln Glu Ala Lys Leu Gln Ala Lys His Asn Asp Lys Lys Ser Lys 
    450                 455                 460                 


Phe Leu Pro Pro Phe Cys Gly Ser Ile Tyr Ala Asp Glu Leu Thr Asn 
465                 470                 475                 480 


Pro Val Val His Arg Ala Ile Ala Glu Tyr Arg Lys Ile Leu Asn Ala 
                485                 490                 495     


Leu Ile Ala Lys Tyr Gly Asn Val His Lys Ile His Ile Glu Leu Thr 
            500                 505                 510         


Arg Asp Val Gly Lys Asn Phe Gln Glu Arg Glu Lys Tyr Lys Lys Glu 
        515                 520                 525             


Ile Glu Ser Asn Tyr Lys Ala Arg Val Gln Ala Met Gln Glu Cys Glu 
    530                 535                 540                 


Lys Leu Gly Leu Thr Leu Ser Glu Gly Asn Ile Leu Lys Leu Arg Leu 
545                 550                 555                 560 


Phe Met Glu Gln Asn Glu Arg Cys Val Tyr Ser Gly Arg Lys Ile Thr 
                565                 570                 575     


Leu Ala Asn Leu Lys Glu Gln Gly Ala Leu Glu Ile Asp His Ile Leu 
            580                 585                 590         


Pro Tyr Ser Arg Ser Ser Asp Asp Ser Tyr Met Asn Lys Val Leu Val 
        595                 600                 605             


Phe Thr Asn Glu Asn Gln Asn Lys Gly Asn Lys Thr Pro Tyr Glu Ala 
    610                 615                 620                 


Phe Gly Gly Asp Ser Gln Lys Trp Gly Glu Ile Glu Ser Leu Ala Leu 
625                 630                 635                 640 


Arg Ser Gly Tyr Pro Lys Lys Lys Ala Lys Arg Ile Leu Asp Lys Gly 
                645                 650                 655     


Phe Gly Asp Arg Glu Ala Gly Phe Lys Ser Arg Asn Ile Val Asp Ser 
            660                 665                 670         


Gly Tyr Ile Ala Arg Leu Ile Ala Asn Tyr Thr Lys Glu Tyr Leu Lys 
        675                 680                 685             


Phe Leu Pro Leu Asp Ser His Glu Asn Thr Ala Leu Ile Ala Gly Glu 
    690                 695                 700                 


Lys Asp Ser Lys Ile His Val Glu Ala Val Lys Gly Met Leu Thr Ala 
705                 710                 715                 720 


Thr Met Arg His Phe Trp Gly Leu Gly Ser Lys Asn Arg Tyr Glu His 
                725                 730                 735     


Thr His His Ala Ile Asp Ala Ile Ile Ile Ala Tyr Ile Asn Ala Ala 
            740                 745                 750         


Met Ile Gln Arg Phe Ser Gln Phe Arg Gln Asn Gln Glu Ser Leu Lys 
        755                 760                 765             


Ala Arg Phe Tyr Ala Lys Glu Leu Ala Lys Glu Glu Phe Lys Thr Gln 
    770                 775                 780                 


Arg Ala Phe Phe Glu Pro Phe Ser Gly Phe Arg Glu Gln Val Leu Ala 
785                 790                 795                 800 


Lys Val Gly Gln Ile Phe Val Ser Arg Pro Pro Arg Lys Arg Ala Arg 
                805                 810                 815     


Gly Ala Leu His Glu Glu Thr Phe Tyr Ser Ile Asp Asp Lys Lys Leu 
            820                 825                 830         


Ser Glu Thr Tyr Gly Gly Lys Glu Gly Val Gln Arg Ala Leu Ser Leu 
        835                 840                 845             


Gly Lys Ile Arg Gln Ile Gly Thr Lys Ile Val Ala Asn Gly Pro Met 
    850                 855                 860                 


Val Arg Val Asp Ile Phe Lys His Lys Lys Ser Gly Arg Phe Tyr Ala 
865                 870                 875                 880 


Val Pro Ile Tyr Thr Met Asp Phe Ala Leu Gly Ile Leu Pro Asn Lys 
                885                 890                 895     


Ala Val Val Ser Gly Lys Asp Asn Asp Arg Ile Ile Lys Asp Trp Leu 
            900                 905                 910         


Glu Met Asp Ser Asn Tyr Glu Phe Cys Phe Ser Leu Phe Lys Asp Asp 
        915                 920                 925             


Val Ile Glu Val Gln Lys Lys Asp Met Glu Lys Pro Glu Leu Ala Tyr 
    930                 935                 940                 


Phe Val Ser Leu Asn Thr Ser Ser Ala Ser Ile Ala Leu Val Lys His 
945                 950                 955                 960 


Asp Asn Lys Phe Glu Thr Leu Thr Ser Asn Gln Ala Lys Leu Phe Ser 
                965                 970                 975     


Asn Ala Lys Glu Asn Asn Val Ile Ala Glu Ser Leu Gly Ile Gln Asn 
            980                 985                 990         


Leu Lys Val Phe Lys Lys Tyr Lys  Val Ser Pro Leu Gly  Glu Ile Lys 
        995                 1000                 1005             


Glu Ala  Lys Tyr Glu Pro Arg  Gln Pro Ile Ala Leu  Lys Thr Ser 
    1010                 1015                 1020             


Pro Lys  Lys His Arg Lys Gln  Asn Asn Ala Gln 
    1025                 1030                 


<210>  496
<211>  1035
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  496

Met Arg Arg Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp Cys 
1               5                   10                  15      


Leu Leu Asp Leu Gly Asp Asp Gly Glu Pro Val Ser Ile Phe Arg Thr 
            20                  25                  30          


Gly Ala Arg Ile Phe Ser Asp Gly Arg Asp Pro Lys Ser Leu Gly Ser 
        35                  40                  45              


Leu Lys Ala Thr Arg Arg Glu Ala Arg Leu Thr Arg Arg Arg Arg Asp 
    50                  55                  60                  


Arg Phe Ile Gln Arg Gln Lys Asn Leu Ile Asn Ala Leu Val Lys Tyr 
65                  70                  75                  80  


Gly Leu Met Pro Ala Asp Glu Ile Gln Arg Gln Ala Leu Ala Tyr Lys 
                85                  90                  95      


Asp Pro Tyr Pro Ile Arg Lys Lys Ala Leu Asp Glu Ala Ile Asp Pro 
            100                 105                 110         


Tyr Glu Met Gly Arg Ala Ile Phe His Ile Asn Gln Arg Arg Gly Phe 
        115                 120                 125             


Lys Ser Asn Arg Lys Ser Ala Asp Asn Glu Ala Gly Val Val Lys Gln 
    130                 135                 140                 


Ser Ile Ala Asp Leu Glu Met Lys Leu Gly Glu Ala Gly Ala Arg Thr 
145                 150                 155                 160 


Ile Gly Glu Phe Leu Ala Asp Arg Gln Ala Thr Asn Asp Thr Val Arg 
                165                 170                 175     


Ala Arg Arg Leu Ser Gly Thr Asn Ala Leu Tyr Glu Phe Tyr Pro Asp 
            180                 185                 190         


Arg Tyr Met Leu Glu Gln Glu Phe Asp Thr Leu Trp Ala Lys Gln Ala 
        195                 200                 205             


Ala Phe Asn Pro Ser Leu Tyr Ile Glu Ala Ala Arg Glu Arg Leu Lys 
    210                 215                 220                 


Glu Ile Val Phe Phe Gln Arg Lys Leu Lys Pro Gln Glu Val Gly Arg 
225                 230                 235                 240 


Cys Ile Phe Leu Ser Asp Glu Asp Arg Ile Ser Lys Ala Leu Pro Ser 
                245                 250                 255     


Phe Gln Arg Phe Arg Ile Tyr Gln Glu Leu Ser Asn Leu Ala Trp Ile 
            260                 265                 270         


Asp His Asp Gly Val Ala His Arg Ile Thr Ala Ser Leu Ala Leu Arg 
        275                 280                 285             


Asp His Leu Phe Asp Glu Leu Glu His Lys Lys Lys Leu Thr Phe Lys 
    290                 295                 300                 


Ala Met Arg Ala Ile Leu Arg Lys Gln Gly Val Val Asp Tyr Pro Val 
305                 310                 315                 320 


Gly Phe Asn Leu Glu Ser Asp Asn Arg Asp His Leu Ile Gly Asn Leu 
                325                 330                 335     


Thr Ser Cys Ile Met Arg Asp Ala Lys Lys Met Ile Gly Ser Ala Trp 
            340                 345                 350         


Asp Arg Leu Asp Glu Glu Glu Gln Asp Ser Phe Ile Leu Met Leu Gln 
        355                 360                 365             


Asp Asp Gln Lys Gly Asp Asp Glu Val Arg Ser Ile Leu Thr Gln Gln 
    370                 375                 380                 


Tyr Gly Leu Ser Asp Asp Val Ala Glu Asp Cys Leu Asp Val Arg Leu 
385                 390                 395                 400 


Pro Asp Gly His Gly Ser Leu Ser Lys Lys Ala Ile Asp Arg Ile Leu 
                405                 410                 415     


Pro Val Leu Arg Asp Gln Gly Leu Ile Tyr Tyr Asp Ala Val Lys Glu 
            420                 425                 430         


Ala Gly Leu Gly Glu Ala Asn Leu Tyr Asp Pro Tyr Ala Ala Leu Ser 
        435                 440                 445             


Asp Lys Leu Asp Tyr Tyr Gly Lys Ala Leu Ala Gly His Val Met Gly 
    450                 455                 460                 


Ala Ser Gly Lys Phe Glu Asp Ser Asp Glu Lys Arg Tyr Gly Thr Ile 
465                 470                 475                 480 


Ser Asn Pro Thr Val His Ile Ala Leu Asn Gln Val Arg Ala Val Val 
                485                 490                 495     


Asn Glu Leu Ile Arg Leu His Gly Lys Pro Asp Glu Val Val Ile Glu 
            500                 505                 510         


Ile Gly Arg Asp Leu Pro Met Gly Ala Asp Gly Lys Arg Glu Leu Glu 
        515                 520                 525             


Arg Phe Gln Lys Glu Gly Arg Ala Lys Asn Glu Arg Ala Arg Asp Glu 
    530                 535                 540                 


Leu Lys Lys Leu Gly His Ile Asp Ser Arg Glu Ser Arg Gln Lys Phe 
545                 550                 555                 560 


Gln Leu Trp Glu Gln Leu Ala Lys Glu Pro Val Asp Arg Cys Cys Pro 
                565                 570                 575     


Phe Thr Gly Lys Met Met Ser Ile Ser Asp Leu Phe Ser Asp Lys Val 
            580                 585                 590         


Glu Ile Glu His Leu Leu Pro Phe Ser Leu Thr Leu Asp Asp Ser Met 
        595                 600                 605             


Ala Asn Lys Thr Val Cys Phe Arg Gln Ala Asn Arg Asp Lys Gly Asn 
    610                 615                 620                 


Arg Ala Pro Phe Asp Ala Phe Gly Asn Ser Pro Ala Gly Tyr Asp Trp 
625                 630                 635                 640 


Gln Glu Ile Leu Gly Arg Ser Gln Asn Leu Pro Tyr Ala Lys Arg Trp 
                645                 650                 655     


Arg Phe Leu Pro Asp Ala Met Lys Arg Phe Glu Ala Asp Gly Gly Phe 
            660                 665                 670         


Leu Glu Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser Arg Tyr Thr Thr 
        675                 680                 685             


Glu Tyr Ile Ser Thr Ile Ile Pro Lys Asn Lys Ile Trp Val Val Thr 
    690                 695                 700                 


Gly Arg Leu Thr Ser Leu Leu Arg Gly Phe Trp Gly Leu Asn Ser Ile 
705                 710                 715                 720 


Leu Arg Gly His Asn Thr Asp Asp Gly Thr Pro Ala Lys Lys Ser Arg 
                725                 730                 735     


Asp Asp His Arg His His Ala Ile Asp Ala Ile Val Val Gly Met Thr 
            740                 745                 750         


Ser Arg Gly Leu Leu Gln Lys Val Ser Lys Ala Ala Arg Arg Ser Glu 
        755                 760                 765             


Asp Leu Asp Leu Thr Arg Leu Phe Glu Gly Arg Ile Asp Pro Trp Asp 
    770                 775                 780                 


Gly Phe Arg Asp Glu Val Lys Lys His Ile Asp Ala Ile Ile Val Ser 
785                 790                 795                 800 


His Arg Pro Arg Lys Lys Ser Gln Gly Ala Leu His Asn Asp Thr Ala 
                805                 810                 815     


Tyr Gly Ile Val Glu His Ala Glu Asn Gly Ala Ser Thr Val Val His 
            820                 825                 830         


Arg Val Pro Ile Thr Ser Leu Gly Lys Gln Ser Asp Ile Glu Lys Val 
        835                 840                 845             


Arg Asp Pro Leu Ile Lys Ser Ala Leu Leu Asn Glu Thr Ala Gly Leu 
    850                 855                 860                 


Ser Gly Lys Ser Phe Glu Asn Ala Val Gln Lys Trp Cys Ala Asp Asn 
865                 870                 875                 880 


Ser Ile Lys Ser Leu Arg Ile Val Glu Thr Val Ser Ile Ile Pro Ile 
                885                 890                 895     


Thr Asp Lys Glu Gly Val Ala Tyr Lys Gly Tyr Lys Gly Asp Gly Asn 
            900                 905                 910         


Ala Tyr Met Asp Ile Tyr Gln Asp Pro Thr Ser Ser Lys Trp Lys Gly 
        915                 920                 925             


Glu Ile Val Ser Arg Phe Asp Ala Asn Gln Lys Gly Phe Ile Pro Ser 
    930                 935                 940                 


Trp Gln Ser Gln Phe Pro Thr Ala Arg Leu Ile Met Arg Leu Arg Ile 
945                 950                 955                 960 


Asn Asp Leu Leu Lys Leu Gln Asp Gly Glu Ile Glu Glu Ile Tyr Arg 
                965                 970                 975     


Val Gln Arg Leu Ser Gly Ser Lys Ile Leu Met Ala Pro His Thr Glu 
            980                 985                 990         


Ala Asn Val Asp Ala Arg Asp Arg  Asp Lys Asn Asp Thr  Phe Lys Leu 
        995                 1000                 1005             


Thr Ser  Lys Ser Pro Gly Lys  Leu Gln Ser Ala Ser  Ala Arg Lys 
    1010                 1015                 1020             


Val His  Ile Ser Pro Thr Gly  Leu Ile Arg Glu Gly  
    1025                 1030                 1035 


<210>  497
<211>  1035
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  497

Met Glu Gln Leu Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Val Glu Ile Asp His Asn Glu Asp Pro Ile Arg 
            20                  25                  30          


Leu Ile Asp Val Gly Val Arg Thr Phe Asn Lys Ala Glu Val Ala Lys 
        35                  40                  45              


Thr Gly Glu Ser Leu Ser Leu Ile Arg Arg Leu Ala Arg Gly Ser Arg 
    50                  55                  60                  


Arg Leu Val Arg Arg Lys Lys Met Arg Leu Leu Thr Ala Lys Arg Leu 
65                  70                  75                  80  


Leu Lys Ser Glu Gly Ile Leu Ser Asp Leu Asp Phe Asn Lys Gly Ala 
                85                  90                  95      


Ile Leu Asn Leu Pro Asn Asn Cys Trp Glu Leu Arg Val Lys Gly Leu 
            100                 105                 110         


Asp Lys Lys Leu Ser Asp Lys Glu Trp Ala Ala Val Leu Leu His Ile 
        115                 120                 125             


Ile Lys His Arg Gly Tyr Leu Ser Gln Arg Lys His Asp Arg Lys Thr 
    130                 135                 140                 


Asp Asp Lys Glu Leu Gly Ala Leu Leu Ser Gly Val Ser Lys Asn His 
145                 150                 155                 160 


Ser Leu Leu Glu Glu Gly Glu Phe Arg Thr Ala Ala Glu Leu Ala Ile 
                165                 170                 175     


Asn Val Phe Cys Asn Ser Lys Gly His Ile Arg Asn Gln Trp Gly Asp 
            180                 185                 190         


Tyr Ser His Thr Phe Ser Arg Leu Asp Leu Gln Lys Glu Ile Lys Gln 
        195                 200                 205             


Leu Phe Val Ser Gln Arg Glu Leu Gly Asn Pro Tyr Thr Ser Ser Ser 
    210                 215                 220                 


Phe Glu Glu Asn Ile Ile Ser Leu Leu Met Lys Gln Lys Glu Ala Ile 
225                 230                 235                 240 


Ser Gly Asn Asp Ile Leu Lys Met Leu Gly Lys Cys Thr Tyr Glu Lys 
                245                 250                 255     


Asn Glu Tyr Lys Ala Ala Lys Asn Thr Tyr Ser Ala Glu Arg Phe Val 
            260                 265                 270         


Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile Val Asp Asp Gly Val Glu 
        275                 280                 285             


Arg Ala Leu Asn Ser Ser Glu Arg Lys Leu Leu Ile Asp Glu Pro Tyr 
    290                 295                 300                 


Lys Lys Ala Lys Leu Thr Tyr Ala Gln Val Arg Lys Ile Leu Glu Leu 
305                 310                 315                 320 


Pro Asp Ser Ala Val Phe Lys Gly Val Arg Tyr Phe Ala Asp Asn Ala 
                325                 330                 335     


Glu Ser Ser Thr Leu Met Glu Met Lys Phe Trp His Ser Ile Arg Lys 
            340                 345                 350         


Ala Leu Glu Ser Asn Asn Leu Lys Asp Glu Trp Leu Val Leu Lys Thr 
        355                 360                 365             


Lys Pro Glu Leu Leu Asp Glu Ile Gly Thr Gly Phe Ser Ile Tyr Lys 
    370                 375                 380                 


Thr Asp Asp Asp Ile Thr Ser Tyr Leu Ser Asn Lys Leu Asp Lys Lys 
385                 390                 395                 400 


Val Leu Asp Ile Leu Leu Val Glu Val Ser Phe Asp Lys Phe Ile Glu 
                405                 410                 415     


Leu Ser Leu Lys Cys Leu Arg Lys Ile Ile Pro Leu Met Glu Lys Gly 
            420                 425                 430         


Tyr Arg Tyr Asp Glu Ala Tyr Lys Tyr Ile Tyr Gly Glu Val Asn Ile 
        435                 440                 445             


Glu Tyr Asn Leu Asn Asp Ile Lys Asn Pro Val Val Leu Arg Ser Phe 
    450                 455                 460                 


Ser Gln Ser Arg Lys Val Ile Asn Ala Val Ile Arg Leu Tyr Gly Ser 
465                 470                 475                 480 


Pro Ala Arg Ile His Ile Glu Thr Ala Arg Asp Ile Gly Lys Ser Phe 
                485                 490                 495     


Lys Glu Arg Lys Ala Ile Glu Lys Gln Gln Glu Lys Asn Arg Leu Glu 
            500                 505                 510         


Lys Val Arg Ala Ala Glu His Phe Lys Gln Leu Phe Pro Asn Tyr Val 
        515                 520                 525             


Gly Glu Pro Lys Ala Lys Asp Ile Leu Lys Val Arg Leu Tyr Glu Gln 
    530                 535                 540                 


Gln Gln Gly Lys Cys Leu Tyr Ser Gly Lys Ile Leu Asp Ile Lys Arg 
545                 550                 555                 560 


Leu Met Glu His Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe Ser 
                565                 570                 575     


Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Ala Asn 
            580                 585                 590         


Glu Asn Gln Asn Lys Gly Asn Lys Thr Pro Tyr Glu Trp Phe Asn Gly 
        595                 600                 605             


Lys Asp Asn Ser Asp Ser Trp Lys Arg Phe Leu Ala Leu Val Asn Asn 
    610                 615                 620                 


Ser Gly Phe Ser Ala Val Lys Lys Gln His Ile Leu Met Lys Glu Ile 
625                 630                 635                 640 


Asp Glu Glu Gly Phe Lys Gln Arg Asn Leu Asn Asp Thr Arg Tyr Ile 
                645                 650                 655     


Thr Arg Phe Leu Cys Asn Trp Val Glu Asn Asn Ile Pro Leu Leu Gly 
            660                 665                 670         


Lys Gly Val Lys Arg Val Phe Ala Pro Gln Gly Lys Ile Thr Ala Leu 
        675                 680                 685             


Leu Arg Ser Asn Trp Gly Ile Arg Lys Val Arg Glu Asp Asn Asp Arg 
    690                 695                 700                 


His His Ala Ile Asp Ala Ile Val Val Ala Cys Thr Thr Val Ser Ile 
705                 710                 715                 720 


Gln Gln Arg Ile Thr Asn Tyr Val Lys Asn Ser Glu Ile Asn Arg Lys 
                725                 730                 735     


Ile Lys Arg Gln Asn Tyr Leu Lys Phe Pro Glu Pro Trp Lys Phe Phe 
            740                 745                 750         


Arg Glu Asp Ile Ser Ile Arg Val Phe Ser Asn Asn Pro Ile Glu Glu 
        755                 760                 765             


Leu Lys Gln Gln Leu Ser Asp Arg Pro Glu Ala Asn His Gln Phe Val 
    770                 775                 780                 


Thr Pro Leu Phe Val Ser Arg Ala Pro Val Arg Lys Met Thr Gly Gln 
785                 790                 795                 800 


Gly His Glu Asp Thr Ile Arg Ser Ala Lys Arg Ile Ser Glu Gly Ile 
                805                 810                 815     


Ser Val Leu Arg Val Pro Leu Thr Lys Leu Asn Asp Lys Ser Leu Glu 
            820                 825                 830         


Leu Met Val Asn Arg Glu Arg Glu Lys Glu Leu Tyr Asp Ala Leu Lys 
        835                 840                 845             


Gln Arg Leu Leu Glu Phe Lys Asn Asp Pro Glu Lys Ala Phe Ala Glu 
    850                 855                 860                 


Pro Phe Tyr Lys Lys Gly Gly Gln Gln Val Lys Thr Val Arg Ile Glu 
865                 870                 875                 880 


Arg Val Gln Lys Asn Gly Val Leu Val Asn Asn Asn Thr Gly Ile Ala 
                885                 890                 895     


Asp Asn Gly Asn Ile Val Arg Val Asp Val Phe Leu Lys Lys Lys Lys 
            900                 905                 910         


Tyr Phe Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu 
        915                 920                 925             


Pro Asn Lys Ala Ile Val Ala His Lys Asn Glu Cys Asp Trp Ala Asp 
    930                 935                 940                 


Met Asp Gly Ser Ala Gln Phe Leu Phe Ser Leu Tyr Thr Asn Asp Leu 
945                 950                 955                 960 


Ile Glu Val Val Leu Lys Asp Ser Thr Ile Leu Gly Tyr Phe Ser Ser 
                965                 970                 975     


Ile Asp Arg Ala Thr Ala Ala Ile Ile Ile Lys Glu Pro Asp Met Glu 
            980                 985                 990         


Lys Ser Lys Gly Lys Glu Gly Ile  His Arg Val Gly Val  Lys Thr Ala 
        995                 1000                 1005             


Arg Ser  Phe Ile Lys Tyr Asn  Val Asp Val Leu Gly  Lys Asn Ile 
    1010                 1015                 1020             


Ser Arg  Cys Arg Lys Ala Lys  Arg Leu Pro Val Arg  
    1025                 1030                 1035 


<210>  498
<211>  1035
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  498

Met Leu Arg Lys Met His Tyr Arg Leu Ala Leu Asp Leu Gly Ser Thr 
1               5                   10                  15      


Ser Leu Gly Trp Ala Met Ile Arg Leu Asn Ala Gly Asn Glu Pro Cys 
            20                  25                  30          


Ala Val Ile Lys Ala Gly Val Arg Ile Phe Ser Asn Gly Arg Asn Pro 
        35                  40                  45              


Lys Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Glu Ala Arg Ala Met 
    50                  55                  60                  


Arg Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Ala Arg Met Ile Gln 
65                  70                  75                  80  


Thr Leu Ile Glu Tyr Gly Phe Phe Pro Ala Lys Glu Asp Gln Arg Lys 
                85                  90                  95      


Ala Leu Glu Ile Met Asn Pro Phe Ala Leu Arg Ala Lys Gly Leu Asn 
            100                 105                 110         


Glu Ala Leu Thr Pro Ala Glu Phe Ala Arg Ala Leu Phe His Ile Asn 
        115                 120                 125             


Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Lys Asp Asn 
    130                 135                 140                 


Asp Ser Gly Ala Leu Lys Thr Ala Ile Asn Lys Leu Arg Glu Thr Leu 
145                 150                 155                 160 


Lys Thr Glu Asn Cys Arg Thr Val Gly Glu Trp Leu Asn Lys Arg Asp 
                165                 170                 175     


Val Ala Gly Leu Thr Val Arg Ala Arg Tyr His Gln Asp Lys Thr Ile 
            180                 185                 190         


Lys Glu Asp Gly Lys Ser Lys Ile Asn Lys Tyr Tyr Asp Leu Tyr Ile 
        195                 200                 205             


Asp Arg Ala Met Ile Glu His Glu Phe Asp Glu Leu Trp Lys Lys Gln 
    210                 215                 220                 


Ala Glu Leu Asn Pro Thr Leu Phe Thr Glu Ala Ala Arg Ala Asp Leu 
225                 230                 235                 240 


Lys Asp Val Leu Leu His Gln Arg Ala Leu Lys Pro Val Lys Pro Gly 
                245                 250                 255     


Arg Cys Thr Phe Met Pro Asp Glu Glu Arg Ala Pro Leu Ala Leu Pro 
            260                 265                 270         


Ser Thr Gln Arg Phe Arg Met Tyr Gln Glu Val Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Arg Asp Gly Leu Lys Glu Glu Ser Leu Thr Leu Asn Gln Arg Asp 
    290                 295                 300                 


Asp Leu Ile Ala Ala Leu Glu Glu Asn Asn Lys Arg Thr Phe Thr Gln 
305                 310                 315                 320 


Ile Lys Lys Leu Leu Gly Leu Gly Gly Val Val Lys Phe Asn Phe Glu 
                325                 330                 335     


Asp Pro Lys Arg Glu Glu Leu Lys Gly Asn Thr Thr Ser Ala Ile Leu 
            340                 345                 350         


Gly Lys Asp Glu His Phe Gly Ser Val Trp Phe Lys Phe Asp Glu Ala 
        355                 360                 365             


Lys Gln Asp Ala Ile Val Met Gln Leu Phe Lys Glu Glu Asn Glu Ala 
    370                 375                 380                 


Lys Leu Leu Arg Trp Leu Gln Asn Glu Thr Gly Ile Asp Glu Lys Arg 
385                 390                 395                 400 


Ala Glu Thr Ile Ala Asn Val Gly Leu Pro Glu Gly Tyr Gly Ser Leu 
                405                 410                 415     


Cys Ala Leu Ala Leu Ala Arg Ile Leu Pro Glu Leu Arg Arg Asp Val 
            420                 425                 430         


Val Pro Tyr Ser Asp Ala Ala Lys Ala Ala Gly Phe His His Ser Asp 
        435                 440                 445             


Thr Ser Asn Asn Gln Glu Val Pro Gly Leu Thr Phe Pro Ile Glu Leu 
    450                 455                 460                 


Val Val Thr Glu Thr Gly Glu Ile Lys Thr Lys His Ile Phe His Glu 
465                 470                 475                 480 


Leu Pro Tyr Tyr Gly Glu Tyr Leu Gln Arg His Val Gly Phe Gly Ser 
                485                 490                 495     


Gly Lys Pro Glu Asp Ser Ala Glu Lys Arg Tyr Gly Lys Ile Ala Asn 
            500                 505                 510         


Pro Thr Val His Ile Gly Leu Asn Gln Val Arg Leu Val Val Asn Thr 
        515                 520                 525             


Leu Ile Lys Arg Tyr Gly His Pro Ser Glu Val Ile Val Glu Val Ala 
    530                 535                 540                 


Arg Asp Leu Lys Gln Ser Lys Asp Leu Arg Asp Glu Glu Gly Lys Arg 
545                 550                 555                 560 


Gln Ala Glu Asn Gln Lys Arg Asn Lys Arg Ile Arg Thr Ala Ile Ala 
                565                 570                 575     


Gly Val Leu Gln Thr Ser Glu Glu Arg Val Arg Arg Asp Asp Ile Glu 
            580                 585                 590         


Lys Met Ile Leu Trp Glu Glu Leu Ser Phe Asp Pro Ala Asp Arg Arg 
        595                 600                 605             


Cys Pro Tyr Ser Gly Val Gln Ile Ser Ala Ala Met Leu Leu Ser Asp 
    610                 615                 620                 


Glu Val Glu Ile Glu His Ile Leu Pro Phe Ser Gln Thr Leu Asp Asp 
625                 630                 635                 640 


Ser Leu Asn Asn Lys Thr Val Ala Leu Arg Gln Ala Asn Arg Val Lys 
                645                 650                 655     


Gly Asn Arg Thr Pro Trp Glu Ala Phe Gly Ala Gln Ser Thr Ala Gly 
            660                 665                 670         


Phe Asp Tyr Ala Thr Ile Leu Ala Arg Ala Glu His Met Pro Lys Gly 
        675                 680                 685             


Lys Arg Tyr Arg Phe Ala Glu Asp Gly Tyr Gln Arg Trp Leu Lys Asp 
    690                 695                 700                 


Asp Ala Gly Phe Leu Pro Arg Ala Leu Asn Asp Thr Arg His Met Ser 
705                 710                 715                 720 


Lys Val Ala Arg Glu Tyr Met Ser Leu Ile Cys Pro Asn Thr Arg Val 
                725                 730                 735     


Ile Pro Gly Arg Met Thr Ala Met Leu Arg Gly Lys Phe Gly Leu Asn 
            740                 745                 750         


Asp Val Leu Gly Leu Asn Gly Glu Lys Asn Arg Asn Asp His Arg His 
        755                 760                 765             


His Ala Val Asp Ala Cys Val Ile Ala Val Thr Asp Gln Gly Leu Leu 
    770                 775                 780                 


Gln Arg Phe Ala Lys Ala Ser Ala Ser Ala Arg Glu Gln Gln Leu Asn 
785                 790                 795                 800 


Arg Leu Val Glu Asn Met Pro Leu Pro Trp Glu Ser Tyr Arg Glu His 
                805                 810                 815     


Val Glu Arg Ala Ile Asp Ala Ile Trp Val Ser His Lys Pro Asp His 
            820                 825                 830         


Ser His Glu Gly Ala Met His Asn Asp Thr Ala Tyr Gly Leu Leu Gly 
        835                 840                 845             


Asp Gly Lys Val Ser Ile His Lys Met Val Glu Gly Lys Arg Glu Arg 
    850                 855                 860                 


Ile Glu Glu Ser Leu Lys Val Ile Ala Phe Thr Asn Ala Lys Ala Asn 
865                 870                 875                 880 


Asp Arg His Gly Leu Leu Asp Asn Gly Glu Pro Arg Pro Tyr Lys Gly 
                885                 890                 895     


Tyr Lys Gly Asp Ser Asn Tyr Cys Ile Glu Ile Ile Arg Asn Glu Lys 
            900                 905                 910         


Gly Lys Trp Glu Gly Glu Val Ile Ser Thr Phe Asp Ala Tyr Gln Leu 
        915                 920                 925             


Val Arg Glu His Gly Val Ala Arg Leu Arg His Pro Thr Leu Ser Val 
    930                 935                 940                 


Ser Gly Lys Pro Leu Val Met Arg Leu Met Lys Asp Glu Thr Ile Arg 
945                 950                 955                 960 


Met Val Thr Asn Glu Lys Pro Ile Thr Ala Arg Val Cys Trp Val Arg 
                965                 970                 975     


Ser Asp Thr Arg Ile Ala Phe Ala Ser Thr Ser Glu Ala Asn Val Asp 
            980                 985                 990         


Ala Arg Asp Arg Asp Lys Asn Asp  Ser Phe Ser Tyr Ile  Val Lys Thr 
        995                 1000                 1005             


Ala Ser  Val Leu Gln Lys Leu  Gln Ala Arg Arg Ile  Ser Ile Ser 
    1010                 1015                 1020             


Pro Thr  Gly Glu Leu Arg Asp  Pro Gly Phe Lys Glu  
    1025                 1030                 1035 


<210>  499
<211>  1035
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  499

Met Glu Gln Leu Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Val Glu Ile Asp His Asn Glu Asp Pro Ile Arg 
            20                  25                  30          


Leu Ile Asp Val Gly Val Arg Thr Phe Asn Lys Ala Glu Val Ala Lys 
        35                  40                  45              


Thr Gly Glu Ser Leu Ser Leu Ile Arg Arg Leu Ala Arg Gly Ser Arg 
    50                  55                  60                  


Arg Leu Val Arg Arg Lys Lys Met Arg Leu Leu Thr Ala Lys Arg Leu 
65                  70                  75                  80  


Leu Lys Ser Glu Gly Ile Leu Ser Asp Leu Asp Phe Asn Lys Gly Ala 
                85                  90                  95      


Ile Leu Asn Leu Pro Asn Asn Cys Trp Glu Leu Arg Val Lys Gly Leu 
            100                 105                 110         


Asp Lys Lys Leu Ser Asp Lys Glu Trp Ala Ala Val Leu Leu His Ile 
        115                 120                 125             


Ile Lys His Arg Gly Tyr Leu Ser Gln Arg Lys His Asp Arg Lys Thr 
    130                 135                 140                 


Asp Asp Lys Glu Leu Gly Ala Leu Leu Ser Gly Val Ser Lys Asn His 
145                 150                 155                 160 


Ser Leu Leu Glu Glu Gly Glu Phe Arg Thr Ala Ala Glu Leu Ala Ile 
                165                 170                 175     


Asn Val Phe Cys Asn Ser Lys Gly His Ile Arg Asn Gln Trp Gly Asp 
            180                 185                 190         


Tyr Ser His Thr Phe Ser Arg Leu Asp Leu Gln Lys Glu Ile Lys Gln 
        195                 200                 205             


Leu Phe Val Ser Gln Arg Glu Leu Gly Asn Pro Tyr Thr Ser Ser Ser 
    210                 215                 220                 


Phe Glu Glu Asn Ile Ile Ser Leu Leu Met Lys Gln Lys Glu Ala Ile 
225                 230                 235                 240 


Ser Gly Asn Asp Ile Leu Lys Met Leu Gly Lys Cys Thr Tyr Glu Lys 
                245                 250                 255     


Asn Glu Tyr Lys Ala Ala Lys Asn Thr Tyr Ser Ala Glu Arg Phe Val 
            260                 265                 270         


Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile Val Asp Asp Gly Val Glu 
        275                 280                 285             


Arg Ala Leu Asn Ser Ser Glu Arg Lys Leu Leu Ile Asp Glu Pro Tyr 
    290                 295                 300                 


Lys Lys Ala Lys Leu Thr Tyr Ala Gln Val Arg Lys Ile Leu Glu Leu 
305                 310                 315                 320 


Pro Asp Ser Ala Val Phe Lys Gly Val Arg Tyr Phe Ala Asp Asn Ala 
                325                 330                 335     


Glu Ser Ser Thr Leu Met Glu Met Lys Phe Trp His Ser Ile Arg Lys 
            340                 345                 350         


Ala Leu Glu Ser Asn Asn Leu Lys Asp Glu Trp Leu Val Leu Lys Thr 
        355                 360                 365             


Lys Pro Glu Leu Leu Asp Glu Ile Gly Thr Gly Phe Ser Ile Tyr Lys 
    370                 375                 380                 


Thr Asp Asp Asp Ile Thr Ser Tyr Leu Ser Asn Lys Leu Asp Lys Lys 
385                 390                 395                 400 


Val Leu Asp Ile Leu Leu Val Glu Val Ser Phe Asp Lys Phe Ile Glu 
                405                 410                 415     


Leu Ser Leu Lys Cys Leu Arg Lys Ile Ile Pro Leu Met Glu Lys Gly 
            420                 425                 430         


Tyr Arg Tyr Asp Glu Ala Tyr Lys Tyr Ile Tyr Gly Glu Val Asn Ile 
        435                 440                 445             


Glu Tyr Asn Leu Asn Asp Ile Lys Asn Pro Val Val Leu Arg Ser Phe 
    450                 455                 460                 


Ser Gln Ser Arg Lys Val Ile Asn Ala Val Ile Arg Leu Tyr Gly Ser 
465                 470                 475                 480 


Pro Ala Arg Ile His Ile Glu Thr Ala Arg Asp Ile Gly Lys Ser Phe 
                485                 490                 495     


Lys Glu Arg Lys Ala Ile Glu Lys Gln Gln Glu Lys Asn Arg Leu Glu 
            500                 505                 510         


Lys Val Arg Ala Ala Glu His Phe Lys Gln Leu Phe Pro Asn Tyr Val 
        515                 520                 525             


Gly Glu Pro Lys Ala Lys Asp Ile Leu Lys Val Arg Leu Tyr Glu Gln 
    530                 535                 540                 


Gln Gln Gly Lys Cys Leu Tyr Ser Gly Lys Ile Leu Asp Ile Lys Arg 
545                 550                 555                 560 


Leu Met Glu His Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe Ser 
                565                 570                 575     


Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Ala Asn 
            580                 585                 590         


Glu Asn Gln Asn Lys Gly Asn Lys Thr Pro Tyr Glu Trp Phe Asn Gly 
        595                 600                 605             


Lys Asp Asn Ser Asp Ser Trp Lys Arg Phe Leu Ala Leu Val Asn Asn 
    610                 615                 620                 


Ser Gly Phe Ser Ala Val Lys Lys Gln His Ile Leu Met Lys Glu Ile 
625                 630                 635                 640 


Asp Glu Glu Gly Phe Lys Gln Arg Asn Leu Asn Asp Thr Arg Tyr Ile 
                645                 650                 655     


Thr Arg Phe Leu Cys Asn Trp Val Glu Asn Asn Ile Pro Leu Leu Gly 
            660                 665                 670         


Lys Gly Val Lys Arg Val Phe Ala Pro Gln Gly Lys Ile Thr Ala Leu 
        675                 680                 685             


Leu Arg Ser Asn Trp Gly Ile Arg Lys Val Arg Glu Asp Asn Asp Arg 
    690                 695                 700                 


His His Ala Ile Asp Ala Ile Val Val Ala Cys Thr Thr Val Ser Ile 
705                 710                 715                 720 


Gln Gln Arg Ile Thr Asn Tyr Val Lys Asn Ser Glu Ile Asn Arg Lys 
                725                 730                 735     


Ile Lys Arg Gln Asn Tyr Leu Lys Phe Pro Glu Pro Trp Lys Phe Phe 
            740                 745                 750         


Arg Glu Asp Ile Ser Ile Arg Val Phe Ser Asn Asn Pro Ile Glu Glu 
        755                 760                 765             


Leu Lys Gln Gln Leu Ser Asp Arg Pro Glu Ala Asn His Gln Phe Val 
    770                 775                 780                 


Thr Pro Leu Phe Val Ser Arg Ala Pro Val Arg Lys Met Thr Gly Gln 
785                 790                 795                 800 


Gly His Glu Asp Thr Ile Arg Ser Ala Lys Arg Ile Ser Glu Gly Ile 
                805                 810                 815     


Ser Val Leu Arg Val Pro Leu Thr Lys Leu Asn Asp Lys Ser Leu Glu 
            820                 825                 830         


Leu Met Val Asn Arg Glu Arg Glu Lys Glu Leu Tyr Asp Ala Leu Lys 
        835                 840                 845             


Gln Arg Leu Leu Glu Phe Lys Asn Asp Pro Glu Lys Ala Phe Ala Glu 
    850                 855                 860                 


Pro Phe Tyr Lys Lys Gly Gly Gln Gln Val Lys Thr Val Arg Ile Glu 
865                 870                 875                 880 


Arg Val Gln Lys Asn Gly Val Leu Val Asn Asn Asn Thr Gly Ile Ala 
                885                 890                 895     


Asp Asn Gly Asn Ile Val Arg Val Asp Val Phe Leu Lys Lys Lys Lys 
            900                 905                 910         


Tyr Phe Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu 
        915                 920                 925             


Pro Asn Lys Ala Ile Val Ala His Lys Asn Glu Cys Asp Trp Ala Asp 
    930                 935                 940                 


Met Asp Gly Ser Ala Gln Phe Leu Phe Ser Leu Tyr Thr Asn Asp Leu 
945                 950                 955                 960 


Ile Glu Val Val Leu Lys Asp Ser Thr Ile Leu Gly Tyr Phe Ser Ser 
                965                 970                 975     


Ile Asp Arg Ala Thr Ala Ala Leu Ile Ile Lys Glu Pro Asp Met Glu 
            980                 985                 990         


Lys Ser Lys Gly Lys Glu Gly Ile  His Arg Val Gly Val  Lys Thr Ala 
        995                 1000                 1005             


Arg Ser  Phe Ile Lys Tyr Asn  Val Asp Val Leu Gly  Lys Asn Ile 
    1010                 1015                 1020             


Ser Arg  Cys Arg Lys Ala Lys  Arg Leu Pro Val Arg  
    1025                 1030                 1035 


<210>  500
<211>  1036
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  500

Met Arg Ile Phe Gly Phe Asp Ile Gly Ile Thr Ser Ile Gly Trp Ala 
1               5                   10                  15      


Tyr Val Glu Ser Asn Glu Leu Lys Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Asn Gly Lys Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Glu Ala Arg Gly Ala Arg Arg Arg Leu Ala Arg Arg Lys Ala Arg Leu 
    50                  55                  60                  


Asn Ala Ile Lys Arg Leu Leu Cys Lys Glu Phe Gly Leu Asn Val Ser 
65                  70                  75                  80  


Asp Tyr Phe Ala Asn Asp Gly Glu Leu Pro Lys Ala Tyr Glu Thr Thr 
                85                  90                  95      


Lys Asp Thr Lys Ser Pro Tyr Glu Leu Arg Ser Leu Ala Leu Glu Gln 
            100                 105                 110         


Lys Leu Glu Pro Lys Glu Leu Ala Arg Val Ile Leu His Ile Ala Lys 
        115                 120                 125             


His Arg Gly Tyr Gly Asn Lys His Ala Lys Thr Gly Ser Asp Lys Asp 
    130                 135                 140                 


Ser Gly Lys Val Leu Ser Ala Ile Lys Val Asn Glu Thr Ala Met Lys 
145                 150                 155                 160 


Asp Lys Tyr Gln Ser Val Gly Glu Tyr Leu Tyr Lys Glu Phe Tyr Gln 
                165                 170                 175     


Lys Leu Arg Leu Glu Ser Glu Lys Lys Gly Asn Asn Ile Thr Lys Glu 
            180                 185                 190         


Phe Lys Asn Val Arg Asn Lys Gly Glu Asn Tyr Glu His Cys Val Ala 
        195                 200                 205             


Gln Glu Gln Val Arg Ala Glu Leu Glu Leu Ile Phe Ala Lys Gln Lys 
    210                 215                 220                 


Asp Phe Gly Ala Thr Phe Ser Glu Asn Phe Glu Ser Gln Ile Ile Glu 
225                 230                 235                 240 


Thr Ala Phe Tyr Gln Arg Asp Leu Lys Ser Phe Glu Asp Lys Val Gly 
                245                 250                 255     


Lys Cys Val Phe Tyr Glu Asn Glu Asn Arg Ala Pro Lys Asp Ser Leu 
            260                 265                 270         


Ser Ala Met Glu Phe Val Ala Leu Thr Arg Ile Ile Asn Thr Leu Lys 
        275                 280                 285             


Asn Leu Glu Glu Lys Ser Lys Asn Leu Gly Ile Gly Glu Val Tyr Gly 
    290                 295                 300                 


Lys Asp Lys Ile Gln Glu Ile Leu Lys Ile Val Leu Asp Lys Gly Glu 
305                 310                 315                 320 


Met Ser Tyr Lys Lys Leu Arg Glu Ile Leu His Leu Asp Glu His Ile 
                325                 330                 335     


Leu Phe Ala Lys Asp Ser Lys Leu Asp Tyr Thr Gln Gly Val Gln Glu 
            340                 345                 350         


Ala Glu Lys Ala Lys Phe Ile Glu Leu Lys Asn Leu Lys Ala Phe Lys 
        355                 360                 365             


Lys Ala Met Gly Gly Asp Phe Ser Asn Phe Ala Arg Glu Glu Leu Asp 
    370                 375                 380                 


Lys Ile Ala Thr Asp Ile Thr Leu Val Lys Ser Lys Glu Asn Leu Ala 
385                 390                 395                 400 


Lys Lys Leu Gln Asn Tyr Ser Ala Leu Ser Lys Glu Gln Val Glu Ala 
                405                 410                 415     


Leu Ser Asn Leu Ser Phe Ala Lys His Ile Asp Leu Ser Leu Lys Ala 
            420                 425                 430         


Leu Glu Gln Ile Leu Pro Phe Met Arg Gly Asp Lys Thr Gly Lys Cys 
        435                 440                 445             


Leu Arg Tyr Asp Glu Ala Val Lys Lys Ala Glu Leu Gln Glu His Arg 
    450                 455                 460                 


Lys His Lys Gln Lys Gly Glu Phe Leu Ile Pro Leu Lys Asp Tyr Glu 
465                 470                 475                 480 


Pro Tyr Leu Ala Asn Pro Val Val Ala Arg Ala Leu Ser Glu Tyr Arg 
                485                 490                 495     


Lys Val Leu Asn Ala Leu Leu Arg Lys Tyr Gly Lys Val His Lys Ile 
            500                 505                 510         


His Leu Glu Phe Thr Arg Glu Ala Lys Leu Ser Ala Lys Glu Arg Gln 
        515                 520                 525             


Lys Tyr Glu Lys Glu Gln Lys Glu His Phe Glu Ala Asn Gln Arg Ala 
    530                 535                 540                 


Ile Gln Gln Cys Glu Ile Leu Gly Leu Pro Ile Asn Gly Thr Asn Ile 
545                 550                 555                 560 


Leu Lys Met Lys Leu Trp Leu Glu Gln Gly Glu Cys Cys Ala Tyr Ser 
                565                 570                 575     


Gly Glu Lys Ile Thr Arg Glu His Leu Leu Asp Ser Asn Ala Leu Gln 
            580                 585                 590         


Val Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met 
        595                 600                 605             


Asn Lys Val Leu Val Phe Ala Lys Glu Asn Gln Asp Lys Gly Asn Lys 
    610                 615                 620                 


Thr Pro Phe Glu Ala Phe Gly Gly Asp Lys Glu Lys Trp Asp Arg Ile 
625                 630                 635                 640 


Leu Ser Leu Ser Ala Lys Leu Pro Lys Gln Lys Gln Arg Arg Ile Ser 
                645                 650                 655     


Asn Thr Asn Phe Lys Asp Lys Glu Gly Gly Phe Leu Ala Arg Asn Leu 
            660                 665                 670         


Asn Asp Thr Gly Tyr Ile Ala Lys Leu Ala Ser Gln Trp Thr Lys Asp 
        675                 680                 685             


Cys Leu Glu Phe Glu Asp Leu Arg Glu Asn Glu Val Thr Ile Ala Gly 
    690                 695                 700                 


Glu Lys Gly Ser Ser Val His Ile Glu Val Val Ser Gly Gln Leu Thr 
705                 710                 715                 720 


Ser Met Leu Arg His Tyr Trp Gly Leu Gly Asn Lys Asp Arg Asn Asn 
                725                 730                 735     


His Leu His His Ala Val Asp Ala Val Ile Leu Ala Tyr Thr Asn Asn 
            740                 745                 750         


Ala Met Ile Lys Ala Phe Ala Asn Phe Arg Lys Asn Gln Glu Glu Asn 
        755                 760                 765             


Lys Lys Lys Phe Tyr Ala Lys Gln Ile Ala Glu Ser Glu Tyr Lys Ala 
    770                 775                 780                 


Lys Lys Ala Phe Phe Glu Pro Cys Lys Asn Phe Arg Gln Arg Val Glu 
785                 790                 795                 800 


Glu Lys Ile Lys Glu Ile Phe Val Ser Lys Pro Pro Arg Lys Arg Val 
                805                 810                 815     


Arg Gly Ala Leu His Gln Glu Thr Phe Tyr Pro Phe Glu Lys Leu Cys 
            820                 825                 830         


Asn Asp Lys Glu Tyr Ser Glu Lys Leu Lys Met Asp Tyr Gly Gly Glu 
        835                 840                 845             


Lys Gly Met Gln Lys Ala Ile Ala Leu Gly Lys Ile Arg Gln Ile Gly 
    850                 855                 860                 


Thr Lys Ile Val Ser Asn Gly Ala Met Val Arg Val Asp Ile Phe Lys 
865                 870                 875                 880 


Asp Lys Lys Gly Lys Phe Tyr Ala Val Pro Ile Tyr Thr Met Asp Phe 
                885                 890                 895     


Ala Leu Gly Ile Leu Pro Asn Lys Ala Val Ala Ile Gly Lys Asp Lys 
            900                 905                 910         


Asp Gly Val Ile Lys Asp Trp Ile Glu Met Asp Ser Asn Tyr Asp Phe 
        915                 920                 925             


Cys Phe Ser Leu Phe Lys Asp Asp Leu Ile Leu Val Gln Lys Lys Glu 
    930                 935                 940                 


Met Glu Arg Ala Glu Leu Cys Tyr Phe Val Ser Phe Gly Ser Thr Asn 
945                 950                 955                 960 


Ala Gln Ile Asn Val Glu Lys His Asn Asn Lys Phe Glu Gly Leu Ser 
                965                 970                 975     


Glu Asn Glu Lys Ile Leu Tyr Ser Asn Ala Thr Gln Lys Glu Val Ala 
            980                 985                 990         


Gly Lys Ser Ile Gly Ile Gln Asn  Leu Lys Thr Phe Glu  Lys Tyr Gln 
        995                 1000                 1005             


Val Ser  Pro Leu Gly Glu Val  Gln Lys Ala Glu Phe  Glu Pro His 
    1010                 1015                 1020             


Gln Glu  Ile Lys Leu Lys Ser  Ser Pro Lys Arg Lys  Asp 
    1025                 1030                 1035     


<210>  501
<211>  1036
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  501

Met Leu His Lys Met His Tyr Arg Leu Ala Leu Asp Leu Gly Ser Thr 
1               5                   10                  15      


Ser Leu Gly Trp Ala Met Ile Arg Leu Asp Ala Asp Asn Gln Pro Cys 
            20                  25                  30          


Ala Val Ile Lys Ala Gly Val Arg Ile Phe Ser Asn Gly Arg Asn Pro 
        35                  40                  45              


Lys Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Glu Ala Arg Ala Met 
    50                  55                  60                  


Arg Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Ala Arg Met Met Arg 
65                  70                  75                  80  


Thr Leu Ile Glu Tyr Gly Phe Phe Pro Ala Glu Ala Ala Gln Arg Lys 
                85                  90                  95      


Ala Leu Glu Thr Ile Asn Pro Phe Ala Leu Arg Ala Lys Gly Leu Asp 
            100                 105                 110         


Glu Ala Leu Thr Pro Ala Glu Phe Gly Arg Ala Leu Phe His Ile Asn 
        115                 120                 125             


Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Lys Asp Asn 
    130                 135                 140                 


Asp Ser Gly Ala Leu Lys Thr Ala Ile Gly Lys Val Arg Ala Thr Ile 
145                 150                 155                 160 


Glu Ala Glu Gly Cys Arg Thr Val Gly Glu Trp Leu His Lys Arg Asp 
                165                 170                 175     


Val Ala Gly Leu Thr Val Arg Ala Arg Tyr His Gln Asp Lys Val Ile 
            180                 185                 190         


Lys Glu Asp Gly Lys Thr Lys Ile Asp Lys Tyr Tyr Asp Leu Tyr Ile 
        195                 200                 205             


Asp Arg Ala Met Ile Glu Gln Glu Phe Asp Glu Leu Trp Lys Lys Gln 
    210                 215                 220                 


Ala Glu Leu Asn Pro Ala Leu Phe Thr Glu Ala Ala Arg Ala Asp Leu 
225                 230                 235                 240 


Lys Asp Val Leu Leu His Gln Arg Pro Leu Lys Pro Val Lys Ser Gly 
                245                 250                 255     


Arg Cys Thr Phe Met Pro Asp Glu Glu Arg Ala Pro Leu Ala Leu Pro 
            260                 265                 270         


Ser Thr Gln Arg Phe Arg Met Tyr Gln Glu Val Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Arg Asp Gly Leu Lys Glu Glu Pro Leu Thr Leu Lys Gln Arg Asp 
    290                 295                 300                 


Asp Leu Ile Ala Ala Leu Glu Lys Asn Ser Lys His Ser Phe Thr Gln 
305                 310                 315                 320 


Ile Lys Thr Leu Leu Gly Val Gly Gly Ala Val Gln Phe Asn Phe Glu 
                325                 330                 335     


Asp Leu Lys Arg Gln Glu Leu Lys Gly Asn Thr Thr Ser Ala Ile Leu 
            340                 345                 350         


Ser Asn Asp Lys His Phe Gly Lys Ala Trp Phe Thr Phe Asp Glu Ala 
        355                 360                 365             


Lys Gln Asp Ala Ile Val Leu Gln Leu Val Lys Glu Glu Asn Glu Ala 
    370                 375                 380                 


Lys Leu Val Arg Trp Leu Gln Asp Glu Thr Gly Ile Asp Glu Lys Arg 
385                 390                 395                 400 


Ala Glu Ala Ile Ala Asn Val Gly Leu Pro Glu Gly Tyr Gly Ser Leu 
                405                 410                 415     


Cys Ala Lys Ala Leu Ala Arg Ile Leu Pro Glu Leu Arg Arg Asp Val 
            420                 425                 430         


Met Thr Tyr Asp Lys Ala Val Gln Ala Ala Gly Phe Glu His His Ser 
        435                 440                 445             


Arg Leu Gly Ser Asn Thr Glu Ile Pro Gly Leu Thr Phe Lys Ile Glu 
    450                 455                 460                 


Ser Ile Asp Gln Asp Ser Gly Glu Val Lys Gln Phe His Ile His Lys 
465                 470                 475                 480 


Val Leu Pro Tyr Tyr Gly Glu Tyr Leu Gln Arg His Val Gly Phe Gly 
                485                 490                 495     


Ser Gly Glu Pro Glu Asp Pro Ala Glu Lys Arg Tyr Gly Lys Ile Ala 
            500                 505                 510         


Asn Pro Thr Val His Ile Gly Leu Asn Gln Ile Arg Leu Val Val Asn 
        515                 520                 525             


Ala Leu Leu Lys Arg Tyr Gly His Pro Ser Glu Val Ile Val Glu Leu 
    530                 535                 540                 


Ala Arg Asp Leu Lys Gln Ser Lys Asp Gln Arg Asp Glu Glu Ser Lys 
545                 550                 555                 560 


Arg Gln Ala Glu Asn Gln Lys Arg Asn Lys Arg Leu Arg Asp Ala Ile 
                565                 570                 575     


Ala Gly Thr Leu Lys Ile Ser Glu Glu Arg Val Arg Arg Asp Asp Ile 
            580                 585                 590         


Gln Lys Met Ile Leu Trp Glu Glu Leu Ser Phe Asp Pro Ala Asp Arg 
        595                 600                 605             


Arg Cys Pro Tyr Ser Gly Ala Gln Ile Ser Ala Thr Met Leu Leu Ser 
    610                 615                 620                 


Asp Glu Val Glu Val Glu His Ile Leu Pro Phe Ser Gln Thr Leu Asp 
625                 630                 635                 640 


Asp Ser Leu Asn Asn Lys Thr Val Ala Leu Arg Gln Ala Asn Arg Ile 
                645                 650                 655     


Lys Gly Asn Arg Thr Pro Trp Asp Ala Phe Gly Ala Gln Ala Val Ala 
            660                 665                 670         


Gly Phe Asp Tyr Glu Ser Ile Leu Val Arg Ala Glu Gly Met Pro Lys 
        675                 680                 685             


Ala Lys Arg Tyr Arg Phe Ala Glu Asn Gly Tyr Gln Arg Trp Leu Lys 
    690                 695                 700                 


Asp Asp Ala Ser Phe Leu Ala Arg Ala Leu Asn Asp Thr Arg His Met 
705                 710                 715                 720 


Ser Lys Val Ala Phe Glu Tyr Leu Lys Leu Ile Cys Pro Asn Thr Arg 
                725                 730                 735     


Val Ile Pro Gly Arg Met Thr Ala Met Leu Arg Ala Lys Phe Gly Leu 
            740                 745                 750         


Asn Asp Val Leu Gly Leu Asn Gly Glu Lys Asn Arg Asn Asp His Arg 
        755                 760                 765             


His His Ala Val Asp Ala Cys Val Ile Ala Val Thr Asp Gln Gly Leu 
    770                 775                 780                 


Leu Gln Arg Phe Ala Lys Ala Ser Ala Ser Ala Arg Glu Gln Gln Leu 
785                 790                 795                 800 


Asn Arg Leu Val Glu Asn Met Pro Leu Pro Trp Gly Ser Tyr Arg Glu 
                805                 810                 815     


His Val Gln Arg Ala Ile Asn Ala Ile Trp Ile Ser His Lys Pro Asp 
            820                 825                 830         


His Ser His Glu Gly Ala Met His Asn Asp Thr Ala Tyr Gly Leu Arg 
        835                 840                 845             


Gly Asp Gly Lys Val Ser Phe His Lys Met Val Glu Gly Val Arg Thr 
    850                 855                 860                 


His Ser Glu Asp Asn Leu Lys Val Ile Glu Ile Ser Asn Ala Lys Ala 
865                 870                 875                 880 


Thr Asp Arg His Gly Leu Leu Pro Asn Gly Glu Pro Arg Pro Tyr Lys 
                885                 890                 895     


Gly Tyr Lys Gly Asp Ser Asn Tyr Cys Ile Glu Ile Val Arg Asn Glu 
            900                 905                 910         


Lys Gly Lys Trp Glu Gly Glu Val Ile Ser Thr Phe Glu Ala Tyr Gln 
        915                 920                 925             


Leu Val Arg Glu His Gly Ile Ala Arg Leu Arg His Ser Thr Leu Ser 
    930                 935                 940                 


Val Ser Gly Lys Leu Leu Val Met Arg Leu Met Ile Asp Asp Val Val 
945                 950                 955                 960 


Arg Leu Ile Ile Asp Glu Gln Thr Arg Thr Met Arg Ile Ala Thr Leu 
                965                 970                 975     


Ser Gly Asn Gly Gln Ile Phe Met Ala Asp Thr Ser Glu Ala Asn Val 
            980                 985                 990         


Asp Ala Arg Asn Arg Ser Lys Glu  Glu Thr Phe Ala Tyr  Val Ser Lys 
        995                 1000                 1005             


Met Ala  Gly Ser Leu Gln Lys  Ala Asn Ala Arg Arg  Ile Asn Ile 
    1010                 1015                 1020             


Ser Pro  Ile Gly Glu Leu Arg  Asp Pro Gly Phe Lys  Glu 
    1025                 1030                 1035     


<210>  502
<211>  1037
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  502

Met Glu Arg Ile Phe Gly Phe Asp Ile Gly Thr Thr Ser Ile Gly Phe 
1               5                   10                  15      


Ser Val Ile Asp Tyr Ser Ser Thr Gln Ser Ala Gly Asn Ile Gln Arg 
            20                  25                  30          


Leu Gly Val Arg Ile Phe Pro Glu Ala Arg Asp Pro Asp Gly Thr Pro 
        35                  40                  45              


Leu Asn Gln Gln Arg Arg Gln Lys Arg Met Met Arg Arg Gln Leu Arg 
    50                  55                  60                  


Arg Arg Arg Ile Arg Arg Lys Ala Leu Asn Glu Thr Leu His Glu Ala 
65                  70                  75                  80  


Gly Phe Leu Pro Ala Tyr Gly Ser Ala Asp Trp Pro Val Val Met Ala 
                85                  90                  95      


Asp Glu Pro Tyr Glu Leu Arg Arg Arg Gly Leu Glu Glu Gly Leu Ser 
            100                 105                 110         


Ala Tyr Glu Phe Gly Arg Ala Ile Tyr His Leu Ala Gln His Arg His 
        115                 120                 125             


Phe Lys Gly Arg Glu Leu Glu Glu Ser Asp Thr Pro Asp Pro Asp Val 
    130                 135                 140                 


Asp Asp Glu Lys Glu Ala Ala Asn Glu Arg Ala Ala Thr Leu Lys Ala 
145                 150                 155                 160 


Leu Lys Asn Glu Gln Thr Thr Leu Gly Ala Trp Leu Ala Arg Arg Pro 
                165                 170                 175     


Pro Ser Asp Arg Lys Arg Gly Ile His Ala His Arg Asn Val Val Ala 
            180                 185                 190         


Glu Glu Phe Glu Arg Leu Trp Glu Val Gln Ser Lys Phe His Pro Ala 
        195                 200                 205             


Leu Lys Ser Glu Glu Met Arg Ala Arg Ile Ser Asp Thr Ile Phe Ala 
    210                 215                 220                 


Gln Arg Pro Val Phe Trp Arg Lys Asn Thr Leu Gly Glu Cys Arg Phe 
225                 230                 235                 240 


Met Pro Gly Glu Pro Leu Cys Pro Lys Gly Ser Trp Leu Ser Gln Gln 
                245                 250                 255     


Arg Arg Met Leu Glu Lys Leu Asn Asn Leu Ala Ile Ala Gly Gly Asn 
            260                 265                 270         


Ala Arg Pro Leu Asp Ala Glu Glu Arg Asp Ala Ile Leu Ser Lys Leu 
        275                 280                 285             


Gln Gln Gln Ala Ser Met Ser Trp Pro Gly Val Arg Ser Ala Leu Lys 
    290                 295                 300                 


Ala Leu Tyr Lys Gln Arg Gly Glu Pro Gly Ala Glu Lys Ser Leu Lys 
305                 310                 315                 320 


Phe Asn Leu Glu Leu Gly Gly Glu Ser Lys Leu Leu Gly Asn Ala Leu 
                325                 330                 335     


Glu Ala Lys Leu Ala Asp Met Phe Gly Pro Asp Trp Pro Ala His Pro 
            340                 345                 350         


Arg Lys Gln Glu Ile Arg His Ala Val His Glu Arg Leu Trp Ala Ala 
        355                 360                 365             


Asp Tyr Gly Glu Thr Pro Asp Lys Lys Arg Val Ile Ile Leu Ser Glu 
    370                 375                 380                 


Lys Asp Arg Lys Ala His Arg Glu Ala Ala Ala Asn Ser Phe Val Ala 
385                 390                 395                 400 


Asp Phe Gly Ile Thr Gly Glu Gln Ala Ala Gln Leu Gln Ala Leu Lys 
                405                 410                 415     


Leu Pro Thr Gly Trp Glu Pro Tyr Ser Ile Pro Ala Leu Asn Leu Phe 
            420                 425                 430         


Leu Ala Glu Leu Glu Lys Gly Glu Arg Phe Gly Ala Leu Val Asn Gly 
        435                 440                 445             


Pro Asp Trp Glu Gly Trp Arg Arg Thr Asn Phe Pro His Arg Asn Gln 
    450                 455                 460                 


Pro Thr Gly Glu Ile Leu Asp Lys Leu Pro Ser Pro Ala Ser Lys Glu 
465                 470                 475                 480 


Glu Arg Glu Arg Ile Ser Gln Leu Arg Asn Pro Thr Val Val Arg Thr 
                485                 490                 495     


Gln Asn Glu Leu Arg Lys Val Val Asn Asn Leu Ile Gly Leu Tyr Gly 
            500                 505                 510         


Lys Pro Asp Arg Ile Arg Ile Glu Val Gly Arg Asp Val Gly Lys Ser 
        515                 520                 525             


Lys Arg Glu Arg Glu Glu Ile Gln Ser Gly Ile Arg Arg Asn Glu Lys 
    530                 535                 540                 


Gln Arg Lys Lys Ala Thr Glu Asp Leu Ile Lys Asn Gly Ile Ala Asn 
545                 550                 555                 560 


Pro Ser Arg Asp Asp Val Glu Lys Trp Ile Leu Trp Lys Glu Gly Gln 
                565                 570                 575     


Glu Arg Cys Pro Tyr Thr Gly Asp Gln Ile Gly Phe Asn Ala Leu Phe 
            580                 585                 590         


Arg Glu Gly Arg Tyr Glu Val Glu His Ile Trp Pro Arg Ser Arg Ser 
        595                 600                 605             


Phe Asp Asn Ser Pro Arg Asn Lys Thr Leu Cys Arg Lys Asp Val Asn 
    610                 615                 620                 


Ile Glu Lys Gly Asn Arg Met Pro Phe Glu Ala Phe Gly His Asp Glu 
625                 630                 635                 640 


Asp Arg Trp Ser Ala Ile Gln Ile Arg Leu Gln Gly Met Val Ser Ala 
                645                 650                 655     


Lys Gly Gly Thr Gly Met Ser Pro Gly Lys Val Lys Arg Phe Leu Ala 
            660                 665                 670         


Lys Thr Met Pro Glu Asp Phe Ala Ala Arg Gln Leu Asn Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ala Lys Gln Ile Leu Ala Gln Leu Lys Arg Leu Trp Pro Asp 
    690                 695                 700                 


Met Gly Pro Glu Ala Pro Val Lys Val Glu Ala Val Thr Gly Gln Val 
705                 710                 715                 720 


Thr Ala Gln Leu Arg Lys Leu Trp Thr Leu Asn Asn Ile Leu Ala Asp 
                725                 730                 735     


Asp Gly Glu Lys Thr Arg Ala Asp His Arg His His Ala Ile Asp Ala 
            740                 745                 750         


Leu Thr Val Ala Cys Thr His Pro Gly Met Thr Asn Lys Leu Ser Arg 
        755                 760                 765             


Tyr Trp Gln Leu Arg Asp Asp Pro Arg Ala Glu Lys Pro Ala Leu Thr 
    770                 775                 780                 


Pro Pro Trp Asp Thr Ile Arg Ala Asp Ala Glu Lys Ala Val Ser Glu 
785                 790                 795                 800 


Ile Val Val Ser His Arg Val Arg Lys Lys Val Ser Gly Pro Leu His 
                805                 810                 815     


Lys Glu Thr Thr Tyr Gly Asp Thr Gly Thr Asp Ile Lys Thr Lys Ser 
            820                 825                 830         


Gly Thr Tyr Arg Gln Phe Val Thr Arg Lys Lys Ile Glu Ser Leu Ser 
        835                 840                 845             


Lys Gly Glu Leu Asp Glu Ile Arg Asp Pro Arg Ile Lys Glu Ile Val 
    850                 855                 860                 


Ala Ala His Val Ala Gly Arg Gly Gly Asp Pro Lys Lys Ala Phe Pro 
865                 870                 875                 880 


Pro Tyr Pro Cys Val Ser Pro Gly Gly Pro Glu Ile Arg Lys Val Arg 
                885                 890                 895     


Leu Thr Ser Lys Gln Gln Leu Asn Leu Met Ala Gln Thr Gly Asn Gly 
            900                 905                 910         


Tyr Ala Asp Leu Gly Ser Asn His His Ile Ala Ile Tyr Arg Leu Pro 
        915                 920                 925             


Asp Gly Lys Ala Asp Phe Glu Ile Val Ser Leu Phe Asp Ala Ser Arg 
    930                 935                 940                 


Arg Leu Ala Gln Arg Asn Pro Ile Val Gln Arg Thr Arg Ala Asp Gly 
945                 950                 955                 960 


Ala Ser Phe Val Met Ser Leu Ala Ala Gly Glu Ala Ile Met Ile Pro 
                965                 970                 975     


Glu Gly Ser Lys Lys Gly Ile Trp Ile Val Gln Gly Val Trp Ala Ser 
            980                 985                 990         


Gly Gln Val Val Leu Glu Arg Asp  Thr Asp Ala Asp His  Ser Thr Thr 
        995                 1000                 1005             


Thr Arg  Pro Met Pro Asn Pro  Ile Leu Lys Asp Asp  Ala Lys Lys 
    1010                 1015                 1020             


Val Ser  Ile Asp Pro Ile Gly  Arg Val Arg Pro Ser  Asn Asp 
    1025                 1030                 1035         


<210>  503
<211>  1038
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  503

Met Ala Lys Gln Val Arg Tyr Arg Leu Ser Leu Asp Leu Gly Ser Thr 
1               5                   10                  15      


Ser Leu Gly Trp Ala Ile Phe Arg Leu Asp Ala Asp Ala His Pro Thr 
            20                  25                  30          


Ala Ile Ile Lys Ala Gly Val Arg Ile Phe Ser Asp Gly Arg Asn Ala 
        35                  40                  45              


Lys Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Ala Ala Arg Ser Met 
    50                  55                  60                  


Arg Arg Arg Arg Asp Arg Leu Leu Arg Arg Lys Thr Arg Met Leu Asp 
65                  70                  75                  80  


Ala Leu Leu Glu Tyr Gly Phe Phe Pro Gln Asp Ala Ala Gln Arg Lys 
                85                  90                  95      


Ala Leu Glu Arg Leu Asn Pro Tyr Ala Leu Arg Ala Glu Gly Leu Ala 
            100                 105                 110         


Arg Pro Leu Thr Pro Gly Glu Phe Ala Arg Ala Ile Phe His Leu Asn 
        115                 120                 125             


Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Asn Asp Asn 
    130                 135                 140                 


Asp Ser Gly Ala Leu Lys Thr Ala Thr Ser Gln Leu Arg Gln Asp Ile 
145                 150                 155                 160 


Gln Ala Ser Gly Cys Arg Thr Val Gly Glu Trp Leu Trp Thr Gln Arg 
                165                 170                 175     


Leu Gln Lys Lys Pro Glu Gly Val Pro His Gln Gly Val Arg Ala Arg 
            180                 185                 190         


Tyr Arg Glu Thr Pro His Ile Asn Ser Glu Gly Lys Lys Arg Ile Glu 
        195                 200                 205             


Lys Lys Tyr Asp Leu Tyr Ile Asp Arg Ser Met Ile Glu Ala Glu Phe 
    210                 215                 220                 


Asp Ala Leu Trp Ser Ala Gln Ala Thr Phe Asn Pro Val Val Phe His 
225                 230                 235                 240 


Ala Thr Ala His Glu Lys Leu Lys Asp Ile Leu Leu Tyr Gln Arg Pro 
                245                 250                 255     


Leu Arg Pro Val Lys Pro Gly Arg Cys Thr Leu Leu Pro Glu Glu Glu 
            260                 265                 270         


Arg Ala Pro Leu Ala Leu Pro Ser Thr Gln Arg Phe Arg Ile Tyr Gln 
        275                 280                 285             


Glu Leu Asn Asn Leu Arg Leu Leu Val Asp Gln Leu Asn Glu Gln Pro 
    290                 295                 300                 


Leu Thr Leu Ala Gln Arg Asp Thr Ile Ala Thr Leu Met Glu Gly Arg 
305                 310                 315                 320 


Ala Lys Ile Ser Phe Asp Gln Ile Arg Lys Ala Leu Lys Leu Gly Ser 
                325                 330                 335     


Met Thr Lys Phe Asn Leu Glu Asp Asp Lys Arg Asn Glu Leu Lys Gly 
            340                 345                 350         


Asn Ala Thr Gly Ser Gln Leu Ala Lys Lys Glu Phe Phe Gly Pro Ala 
        355                 360                 365             


Trp His Gln Phe Asp Glu Ala Leu Gln Asp Asp Ile Val Trp His Leu 
    370                 375                 380                 


Leu Ser Glu Glu Asn Glu Ser Ala Leu Ile Ser Trp Leu Gln Asp Arg 
385                 390                 395                 400 


Thr Gly Ile Asp Ala Ala Thr Ala Gln Ala Val Ser Asn Ala Ser Leu 
                405                 410                 415     


Pro Glu Gly Tyr Gly Ser Leu Ser Arg Lys Ala Leu Glu Val Ile Leu 
            420                 425                 430         


Pro Arg Leu Arg Ser Glu Val Ile Thr Tyr Asp Lys Ala Val Gln Ala 
        435                 440                 445             


Ala Gly Phe Ser His His Ser Gln Leu Gly Phe Glu Tyr Asp Pro Asp 
    450                 455                 460                 


Asp Val Glu Asp Trp Ile Asp His Ala Thr Gly Glu Val Lys Pro Ile 
465                 470                 475                 480 


Phe Lys Arg Leu Pro Tyr Tyr Gly Lys Ala Leu Gln Arg His Val Ala 
                485                 490                 495     


Phe Gly Ser Gly Lys Pro Glu Asp Ser Gln Glu Lys Arg Tyr Gly Lys 
            500                 505                 510         


Ile Ala Asn Pro Thr Val His Ile Gly Leu Asn Gln Val Arg Val Val 
        515                 520                 525             


Ala Asn Ala Leu Ile Ala Arg Tyr Gly Arg Pro Thr Glu Ile Ile Ile 
    530                 535                 540                 


Glu Leu Ala Arg Asp Leu Lys Gln Ser Arg Glu Gln Arg Gln Gln Asp 
545                 550                 555                 560 


Gln Arg Arg Gln Ala Asp Asn Gln Lys Arg Asn Ala Arg Ile Arg Asn 
                565                 570                 575     


Ser Ile Ala Gln Ala Leu Gly Ile Ala Glu Ala Arg Val Arg Arg Glu 
            580                 585                 590         


Asp Ile Gln Lys Trp Ile Leu Trp Glu Glu Leu Asp Pro Gly Asp Ala 
        595                 600                 605             


Met His Arg Cys Cys Pro Tyr Ser Gly Val Pro Ile Ser Ala Ser Met 
    610                 615                 620                 


Leu Leu Ser Glu Gln Val Glu Ile Glu His Ile Leu Pro Phe Ser Met 
625                 630                 635                 640 


Thr Leu Asp Asp Ser Leu Asn Asn Arg Thr Val Ser Leu Arg Gln Ala 
                645                 650                 655     


Asn Arg Ile Lys Arg Asn Arg Ser Pro Trp Asp Ala Arg Glu Asp Phe 
            660                 665                 670         


Glu Ala Gln Gly Trp His Tyr Asp Gly Ile Leu Gln Arg Ala Gly Leu 
        675                 680                 685             


Met Pro Val Gln Lys Arg Tyr Arg Phe Ala Ala Asp Gly Tyr Gln Arg 
    690                 695                 700                 


Trp Leu Arg Asp Asp Gln Asp Phe Leu Ala Arg Ala Leu Asn Asp Thr 
705                 710                 715                 720 


Arg Tyr Leu Ser Arg Ile Ala Ala Glu Tyr Leu Arg Leu Val Cys Pro 
                725                 730                 735     


Gln Gly Thr Arg Val Ile Pro Gly Gln Leu Thr Ala Leu Leu Arg Arg 
            740                 745                 750         


Arg Phe Gly Leu Asn Asp Ile Leu Gly Leu Glu Gly Glu Lys Asn Arg 
        755                 760                 765             


Asn Asp His Arg His His Ala Val Asp Ala Cys Val Ile Gly Ile Thr 
    770                 775                 780                 


Asp His Gly Leu Met Gln Arg Phe Ala Arg Ala Ser Ala Gln Ala Arg 
785                 790                 795                 800 


Glu Gln Gly Leu Thr Arg Leu Ala Glu Glu Met Pro Leu Pro Trp Pro 
                805                 810                 815     


Thr Tyr Arg Asp His Val Asp Arg Ala Val Ser Leu Ile Trp Val Ser 
            820                 825                 830         


His Lys Pro Asp His Gly His Glu Gly Ala Met Met Glu Glu Thr Ala 
        835                 840                 845             


Tyr Gly Leu Met Lys Asp Gly Thr Ile Arg Gln Arg His Lys Ala Asp 
    850                 855                 860                 


Gly Thr Lys Gly Arg Glu Val Ala Asn Val Ile Arg Ile Ala Glu Pro 
865                 870                 875                 880 


Ala Gln Pro Thr Arg His Gly Val Asp Asp Gln Gly Arg Pro Leu Pro 
                885                 890                 895     


Tyr Lys Gly Tyr Ile Gly Gly Ser Asn Tyr Cys Ile Glu Ile Thr Arg 
            900                 905                 910         


Gly Ala Gln Gly Lys Trp Glu Gly Thr Val Ile Ser Thr Ser Asp Ala 
        915                 920                 925             


Tyr Gly Ile Val Arg Ala Arg Gly Ile Ala Gly Leu Arg Asn Thr Ser 
    930                 935                 940                 


Gln Gly Gln Asn Gly His Pro Leu Val Met Arg Leu Val Ile Gly Asp 
945                 950                 955                 960 


Ser Leu Arg Leu Arg Ile Asn Asp Ala Leu Gln Thr Met Arg Val Thr 
                965                 970                 975     


Lys Ile Ser Gly Asn Gly Gln Val Phe Met Ala Gly Val His Glu Ala 
            980                 985                 990         


Asn Val Asp Ala Arg Asn Arg Asp  Lys Ser Gln Ala Phe  Ala Leu Val 
        995                 1000                 1005             


Ser Lys  Met Ala Gly Ser Leu  Gln Ala Ala Gln Ala  Arg Gln Ala 
    1010                 1015                 1020             


Thr Ile  Ser Pro Ile Gly Glu  Ile Arg Asp Pro Gly  Phe Pro Gly 
    1025                 1030                 1035             


<210>  504
<211>  1039
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  504

Met Lys Lys Arg Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Trp Ala Leu Ile Glu Phe Asp Asp Glu Phe Gln Asp Asn Lys Lys Lys 
            20                  25                  30          


Gly Thr Ile Ile Lys Ser Gly Val Arg Ile Phe Thr Arg Ala Glu Thr 
        35                  40                  45              


Pro Lys Glu Arg Glu Pro Leu Ala Lys Pro Arg Arg Glu Ala Arg Ser 
    50                  55                  60                  


Gln Arg Arg Arg Ile Ser Cys Arg Val Glu Arg Leu Asn Phe Val Arg 
65                  70                  75                  80  


Glu Leu Phe Val Lys Asn Gly Leu Val Ala Lys Glu Ser Val Asn Lys 
                85                  90                  95      


Asn Asn Pro Asn Asn Ile Tyr Ile Thr Leu Asn Gln Ile Lys Thr Pro 
            100                 105                 110         


Trp Gln Leu Arg Met Glu Ala Leu Asp Arg Lys Leu Thr Asn Glu Glu 
        115                 120                 125             


Phe Ala Val Val Leu Thr His Leu Ala Lys His Arg Gly Tyr Lys Ser 
    130                 135                 140                 


Leu Arg Lys Thr Asp Ser Lys Asp Asn Lys Thr Gly Glu Met Leu Lys 
145                 150                 155                 160 


Ala Ile Asn Gln Asn Lys Glu Arg Leu Asp Asn Ser Asp Tyr Arg Thr 
                165                 170                 175     


Leu Gly Glu Met Phe Cys Lys His Glu Ser Tyr Lys Glu His Lys Arg 
            180                 185                 190         


Asn Lys Asp Gly Glu Tyr Ile Asn Ser Val Thr Arg Glu Leu Ile Glu 
        195                 200                 205             


Glu Glu Ala Glu Lys Ile Phe Ala Lys Gln Arg Lys Glu Gly Asn Asn 
    210                 215                 220                 


Phe Ala Asp Val Asp Ile Glu Arg Glu Tyr Leu Lys Ile Ala Phe Ser 
225                 230                 235                 240 


Gln Lys Pro Thr Gly Ser Ile Asn Lys Met Val Gly Phe Cys Thr Phe 
                245                 250                 255     


Glu Asn Gly Glu Lys Arg Ala Pro Arg Asp Ser Tyr Ser Ala Glu Leu 
            260                 265                 270         


Phe Lys Val Leu Asn Thr Ile Asn Asn Thr Thr Ile Thr Asp Met Lys 
        275                 280                 285             


Thr Gly Glu Thr Arg Asn Phe Thr Gly Ala Glu Ile Lys Lys Ile Ile 
    290                 295                 300                 


Glu Ile Ile Lys Asp Thr Lys Gly Ile Thr Tyr Val Lys Ile Lys Lys 
305                 310                 315                 320 


Glu Leu Lys Leu Gly Ser Ser Gln Phe Ser Gly Val Asp Tyr Arg Glu 
                325                 330                 335     


Asn Ala Lys Ser Lys Gly Glu Gly Glu Ile Phe Val Ser Met Lys Thr 
            340                 345                 350         


Tyr His Glu Ile Lys Ser Lys Ile Glu Lys Leu Ser Lys Glu Lys Trp 
        355                 360                 365             


Asn Lys Leu Lys Glu Asn Thr Glu Leu Leu Asp Ser Ile Val Asn Ile 
    370                 375                 380                 


Ile Ala Asn Phe Lys Asp Asp Glu Ser Ile Lys Lys Glu Phe Gln Lys 
385                 390                 395                 400 


Leu Lys Ile Asp Glu Asp Ile Ile Asp Val Leu Leu Asn Phe Glu Phe 
                405                 410                 415     


Lys Lys Phe Ile Asn Leu Ser Phe Lys Ala Leu Arg Lys Ile Ile Pro 
            420                 425                 430         


Phe Gln Phe Gln Gly Gln Lys Tyr Ile Thr Ala Ser Met Ser Val Gly 
        435                 440                 445             


Tyr Ser Leu Lys Gln Glu Gly Ile Lys Asn Lys Phe Leu Arg Val Pro 
    450                 455                 460                 


Ser Gln Glu Glu Asn Val Asn Val Pro Val Val Ala Arg Ala Phe Ala 
465                 470                 475                 480 


Gln Thr Arg Lys Val Ile Asn Ala Val Ile Arg Glu Tyr Gly Gln Phe 
                485                 490                 495     


Asp Gln Ile Asn Ile Glu Leu Ala Thr Asp Leu Lys Asn Ser Lys Phe 
            500                 505                 510         


Asp Arg Ser Lys Ile Glu Lys Gly Gln Lys Glu Phe Gln Ser Gln Lys 
        515                 520                 525             


Asp Lys Val Phe Gln Glu Val Gln Asp Leu Thr Gly Glu Ile Ile Pro 
    530                 535                 540                 


Ser Ser Ser Leu Leu Leu Lys Tyr Arg Leu Trp Arg Glu Gln Asp Glu 
545                 550                 555                 560 


Arg Cys Ile Tyr Ser Gly Arg Lys Ile Ser Arg Lys Asp Leu Leu Asp 
                565                 570                 575     


Thr Gly Phe Leu Asp Ile Asp His Ile Ile Pro Tyr Ser Lys Ser Met 
            580                 585                 590         


Asp Asp Gly Phe Asn Asn Lys Val Leu Cys Leu Ala Glu Glu Asn Arg 
        595                 600                 605             


Asn Lys Lys Asn Asp Ile Pro Phe Asn Tyr Tyr Gln Arg Ile Gly Arg 
    610                 615                 620                 


Asp Trp Asn Phe Leu Ile Ser Ile Ile Ala Ser Met Lys Asn Met Lys 
625                 630                 635                 640 


Tyr Val Lys Lys Lys Arg Leu Leu Lys Gln Glu Leu Ser Asp Ile Glu 
                645                 650                 655     


Ile Glu Ser Phe Ile Glu Arg Asn Ile Asn Asp Thr Arg Tyr Ala Thr 
            660                 665                 670         


Arg Tyr Ile Lys Asn Phe Leu Glu Asn Asn Leu Glu Phe Lys Glu Asn 
        675                 680                 685             


Glu Phe Ile Lys Ala Lys Val Gln Ala Arg His Gly Gly Leu Thr Ser 
    690                 695                 700                 


Val Leu Arg Tyr Asn Trp Gly Ile Ser Lys Asn Arg Glu Glu Ser Tyr 
705                 710                 715                 720 


Leu His His Ala Lys Asp Ala Ile Thr Val Ala Cys Ser Thr Gln Gly 
                725                 730                 735     


Met Val Gln Tyr Ile Ser Asn Ile Ser Gln Lys Tyr Glu Asn Gly Lys 
            740                 745                 750         


Tyr Lys Met Lys Gln Ile Lys Glu Arg Thr Glu Asn Arg Ile Pro Lys 
        755                 760                 765             


Pro Trp Asp Ser Phe Lys Asp Asp Val Glu Arg Ser Ile Asn Asp Ile 
    770                 775                 780                 


Phe Val Ser Phe Ala Pro Arg His Lys Val Thr Gly Ser Ala His Lys 
785                 790                 795                 800 


Glu Thr Ile Tyr Ser Lys Lys His Leu Glu Lys Gly Tyr Val Thr Ile 
                805                 810                 815     


Lys Lys Ser Leu Asp Lys Ile Lys Leu Ser Asp Leu Lys Asn Ile Pro 
            820                 825                 830         


Cys Asp Glu Asn Cys Asp Ile Ile Arg Val Leu Lys Glu Arg Leu Glu 
        835                 840                 845             


Lys Phe Asn Asp Asp Ser Leu Lys Ala Phe Ala Asp Pro Val His Met 
    850                 855                 860                 


Pro Thr Lys Cys Ser Asn Lys Lys Gly Pro Ile Ile Arg Ser Val Lys 
865                 870                 875                 880 


Ile Lys Glu Asn Asn Lys Thr Gly Ile Glu Val Arg Lys Gly Leu Val 
                885                 890                 895     


Glu Arg Gly Asp Met Val Arg Val Asp Val Phe Ile Lys Asp Lys Lys 
            900                 905                 910         


Tyr Tyr Leu Val Pro Ile Tyr Val Ser Asp Phe Lys Asn Lys Asp Leu 
        915                 920                 925             


Pro Asn Lys Ala Ile Val Ser Ser Lys Ser Glu Asn Glu Trp Ile Met 
    930                 935                 940                 


Ile Asp Lys Thr Tyr Ser Phe Lys Phe Ser Leu Phe Lys Asp Asp Leu 
945                 950                 955                 960 


Ile Lys Ile Lys Asn Lys Lys Gly Glu Ile Phe Gly Tyr Leu Gly Gly 
                965                 970                 975     


Val His Arg Leu Thr Gly Gly Ile Asn Ile Lys Ser Cys Tyr Lys Pro 
            980                 985                 990         


Lys Glu Lys Asp Gly Asn Ile Gly  Ser Lys Cys Leu Leu  Asp Phe Arg 
        995                 1000                 1005             


Lys Phe  Gln Val Gly Ile Leu  Gly Asp Tyr Asn Glu  Val Lys His 
    1010                 1015                 1020             


Glu Lys  Arg Met Pro Ala Tyr  Ile Asn Thr Lys Lys  Asp Lys Glu 
    1025                 1030                 1035             


His 
    


<210>  505
<211>  1040
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  505

Met Asp Arg Arg Asp Ala Arg Ala Met Arg Arg Arg Arg Asp Arg Phe 
1               5                   10                  15      


Gln Gln Arg Gln Arg Ala Leu Leu Lys Tyr Leu Val Ala Asp Gly Leu 
            20                  25                  30          


Phe Pro Glu Asp Glu Gly Glu Arg Lys Ala Leu Ala Ala Leu Asp Pro 
        35                  40                  45              


Tyr Ala Leu Arg Ala Arg Ala Leu Asp Glu Ala Leu Ser Leu His Glu 
    50                  55                  60                  


Leu Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe Lys Ser 
65                  70                  75                  80  


Asn Arg Lys Ala Asp Arg Lys Ala Asp Asp Asp Ala Gly Lys Ile Ala 
                85                  90                  95      


Ile Gly Val Asp Arg Ala Arg Glu Ala Met Ala Glu Asn Gly Ala Arg 
            100                 105                 110         


Thr Tyr Gly Glu Trp Leu His Arg Arg Arg Ala Ser Ala Thr Asp Ala 
        115                 120                 125             


Asn Ala Ile Pro Pro Val Arg Thr Arg Leu Arg Pro Glu Ser Gly Glu 
    130                 135                 140                 


Asp Ala Lys Gly Asp Gly Tyr Asp Phe Tyr Pro Gly Arg Asp Leu Leu 
145                 150                 155                 160 


Glu Glu Glu Phe Ala Ala Ile Trp Glu Ala Gln Ala Ala His His Pro 
                165                 170                 175     


Asp Val Leu Thr Asp Asp Val Tyr Glu Arg Leu Phe Glu Ile Val Phe 
            180                 185                 190         


Tyr Gln Arg Pro Leu Lys Ala Pro Gln Ile Gly Met Cys Thr Leu Ile 
        195                 200                 205             


Ala Gly Asp Arg Arg Leu Pro Lys Ala His Pro Leu Phe Gln Arg Arg 
    210                 215                 220                 


Arg Leu Leu Glu Glu Leu Asn Ala Leu Met Ile Val Arg Pro Gly Glu 
225                 230                 235                 240 


Val Ala Gln Arg Leu Thr Arg Glu Glu Arg Asp Thr Leu Phe Leu Lys 
                245                 250                 255     


Leu Lys Asp Arg Asn Lys Val Ser Phe Glu Ser Leu Arg Lys Thr Leu 
            260                 265                 270         


Lys Leu Asp Pro Asp Ala Arg Phe Asn Lys Glu Ser Glu Asn Arg Thr 
        275                 280                 285             


Glu Leu Lys Gly Asp Glu Val Ala Ala Ala Met Gly Ser Lys Thr Arg 
    290                 295                 300                 


Phe Gly Asn Arg Trp Val His Leu Ser Ile Glu Glu Gln Trp Ala Val 
305                 310                 315                 320 


Ile Glu Arg Leu Gly Asp Val Glu Ser Asp Ala Glu Asp Ala Ala Phe 
                325                 330                 335     


Arg Ala Trp Leu Ala Glu Arg Tyr Glu Leu Thr Pro Glu Gln Thr Arg 
            340                 345                 350         


Ala Val Thr Asn Ala Pro Leu Pro Gln Gly Tyr Gly Arg Phe Gly Glu 
        355                 360                 365             


Thr Ala Thr Arg Lys Leu Ile Asp Ala Leu Arg Asp Gly Glu Lys Asp 
    370                 375                 380                 


Gly Arg Val Leu Val Tyr Ser Glu Ala Val Thr Ala Ala Gly Leu Gly 
385                 390                 395                 400 


His His Ser Asp Arg Arg Thr Gly Glu Val Phe Glu Asp Glu Glu Gly 
                405                 410                 415     


Arg Pro Gln Leu Pro Tyr Tyr Gly Val Ala Leu Glu Arg His Ile Met 
            420                 425                 430         


Pro Gly Thr Gly Asp Pro Ser Asp Ser Asp Glu Met Arg Val Gly Arg 
        435                 440                 445             


Leu Thr Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu Arg Arg Ile 
    450                 455                 460                 


Val Asn Arg Leu Ile Arg Ala Tyr Gly Pro Pro Ala Glu Ile Ala Ile 
465                 470                 475                 480 


Glu Leu Ala Arg Glu Leu Lys Leu Thr Asp Glu Asp Lys Lys Arg Ile 
                485                 490                 495     


Asn Lys Glu Asn Asn Ser Asn Arg Leu Asp Ala Glu Arg Arg Ser Glu 
            500                 505                 510         


Lys Leu Leu Ala Ile Gly Lys Pro Asp Thr Gly Ala Asn Arg Ala Leu 
        515                 520                 525             


Leu Lys Leu Trp Glu Glu Leu Asn Pro Ala Asn Val Leu Asp Arg Arg 
    530                 535                 540                 


Cys Ile Tyr Ser Gly Val Gln Ile Ser Ile Asp Met Leu Phe Ser Ala 
545                 550                 555                 560 


Ala Val Glu Val Asp His Ile Leu Pro Phe Thr Ala Thr Leu Asp Asp 
                565                 570                 575     


Ser Asn Gly Asn Lys Ile Val Cys Thr Arg Glu Ala Asn Arg Glu Lys 
            580                 585                 590         


Arg Lys Arg Ser Pro Tyr Glu Ala Trp Gly His Ser Ala Arg Trp Glu 
        595                 600                 605             


Glu Ile Ala Ala Arg Ala Ala Gln Leu Pro Arg Asn Lys Arg Trp Arg 
    610                 615                 620                 


Phe Glu Pro Asp Ala Met Glu Lys Phe Lys Asp Asp Gly Gly Phe Gln 
625                 630                 635                 640 


Ala Arg His Leu Ile Asp Thr Gln Tyr Leu Ala Arg Leu Ser Arg Glu 
                645                 650                 655     


Tyr Leu Ala Thr Leu Tyr Pro Asp Lys Gly Glu Gly Ser Gly His Ile 
            660                 665                 670         


Trp Val Ser Pro Gly Arg Leu Thr Glu Met Val Arg Arg Lys Leu Gly 
        675                 680                 685             


Leu Asn Asp Leu Leu Pro Asp His Asn Phe Gly Gly Gly Ala Asp Gln 
    690                 695                 700                 


Ala Lys Asn Arg Leu Asp His Arg His His Ala Ile Asp Ala Val Val 
705                 710                 715                 720 


Val Ala Ile Val Asp Arg Gly Met Leu Asn Arg Ile Ala Arg Ala Ser 
                725                 730                 735     


Gly Gln Glu Gly Ala Glu Gly Arg Glu Arg Ile Ile Ile Pro Glu Pro 
            740                 745                 750         


Trp Glu Gly Phe Arg Asp Asp Leu Arg Thr Ala Val Asn Ala Ile Thr 
        755                 760                 765             


Val Ser His Arg Thr Asp His Gly Thr Ala Ser Lys Ala Gly Leu Pro 
    770                 775                 780                 


Lys Gly Lys Asp Ala Thr Ala Gly Arg Leu His Asn Asp Thr Ala Tyr 
785                 790                 795                 800 


Gly Phe Thr Gly Glu Ser Asp Ala Lys Gly Asn Ser Ile Val Val Arg 
                805                 810                 815     


Arg Val Pro Leu Ser Ser Phe Lys Lys Leu Ala Asp Ile Asp Arg Val 
            820                 825                 830         


Arg Asp Pro Glu Leu Lys Ala Ala Leu Tyr Gln His Leu Asp Gly Leu 
        835                 840                 845             


Glu Gly Lys Ala Phe Glu Lys Ala Ile Gly Glu Phe Gly Ser Arg Glu 
    850                 855                 860                 


Trp His Pro Tyr Gly Gly Val Arg Arg Val Arg Val Thr Glu Pro Leu 
865                 870                 875                 880 


Ser Val Ile Pro Ile Arg Asp Arg Asn Gly His Ile Tyr Lys Gly Tyr 
                885                 890                 895     


Lys Gly Asp Ser Asn Tyr Arg Tyr Asp Val Trp Glu Leu Pro Gly Gly 
            900                 905                 910         


Lys Trp Val Asn Glu Val Val Ser Met Phe Asp Ala His Gln Pro Gly 
        915                 920                 925             


Trp Gln Ser Ala Ile Arg Ala Ala Asn Pro Thr Ala Arg Lys Val Leu 
    930                 935                 940                 


Ser Leu His Arg Asp Asp Val Leu Ala Ile Glu Arg Asn Gly Ala Glu 
945                 950                 955                 960 


Arg Glu Leu Val Arg Val Val Lys Phe Ser Glu Lys Gln Phe Ala Val 
                965                 970                 975     


Ala Pro Pro Asn Glu Gly Gly Ala Leu Lys Ala Arg Asp Ala Asp Lys 
            980                 985                 990         


Gly Asp Pro Phe Lys Tyr Ser Tyr  Pro Ser Pro Asn Thr  Leu Lys Ala 
        995                 1000                 1005             


Leu Arg  Ala Arg Gln Val Arg  Ile Asp Glu Leu Gly  Arg Val Leu 
    1010                 1015                 1020             


Asp Pro  Gly Phe Pro Ala Arg  Thr Lys Gln Arg Lys  Thr Arg Lys 
    1025                 1030                 1035             


Pro Asn  
    1040 


<210>  506
<211>  1043
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  506

Met Gln Arg Asp Asn Pro Leu Val Tyr Arg Leu Ala Leu Asp Leu Gly 
1               5                   10                  15      


Ser Thr Ser Leu Gly Trp Ala Val Phe Arg Leu Asp Ala Glu Gly Ser 
            20                  25                  30          


Pro Leu Ala Ile Ile Lys Ala Gly Ala Arg Ile Phe Ser Asp Gly Arg 
        35                  40                  45              


Asn Pro Lys Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Ala Ala Arg 
    50                  55                  60                  


Ala Met Arg Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Ala Arg Met 
65                  70                  75                  80  


Leu Gly Ala Leu Glu Arg His Gly Phe Phe Pro Gln Asp Glu Ser Ala 
                85                  90                  95      


Arg Lys Val Leu Glu Thr Leu Asn Pro Phe Leu Leu Arg Ala Gln Gly 
            100                 105                 110         


Leu Glu Val Ala Leu Lys Pro Glu Glu Phe Gly Arg Ala Leu Phe His 
        115                 120                 125             


Leu Asn Gln Arg Arg Gly Phe Gln Ser Asn Arg Lys Thr Asp Arg Lys 
    130                 135                 140                 


Asp Ser Asp Ser Gly Ala Leu Lys Gln Ala Ile Gly Thr Leu Arg Ala 
145                 150                 155                 160 


Gln Met His Glu Ser Gly Cys Arg Thr Val Gly Glu Trp Phe Trp Lys 
                165                 170                 175     


Val Arg Met Glu His Lys Pro Glu Gly Val Lys Gly Gln Gly Val Arg 
            180                 185                 190         


Ala Arg Tyr Arg Glu Thr Pro Phe Thr Thr Asp Glu Gly Lys Lys Arg 
        195                 200                 205             


Ile Asp Lys Arg Tyr Asp Leu Tyr Val Asp Arg Ala Met Val Ala Gln 
    210                 215                 220                 


Glu Phe Asp Ala Leu Trp Ala Ala Gln Ala Ala Phe Asn Pro Ala Leu 
225                 230                 235                 240 


Phe Asn Glu Ala Ala Arg Ala Glu Leu Arg Asp Ile Leu Leu His Gln 
                245                 250                 255     


Arg Lys Leu Arg Pro Val Gln Pro Gly Arg Cys Thr Leu Val Pro Glu 
            260                 265                 270         


Glu Pro Arg Ala Leu Leu Ala Leu Ser Ser Thr Gln Arg Phe Arg Ile 
        275                 280                 285             


Tyr Gln Glu Val Asn His Leu Arg Val Leu His Asp Glu Leu Arg Glu 
    290                 295                 300                 


Glu Thr Leu Thr Leu Ala Gln Arg Asp Gln Ile Val Ala Leu Leu Glu 
305                 310                 315                 320 


Lys Gln Ala Lys Val Thr Phe Thr Ala Met Arg Lys Ala Leu Gly Phe 
                325                 330                 335     


Ser Ala Leu Phe Asn Leu Glu Asp Ala Lys Arg Asp Gly Leu Lys Gly 
            340                 345                 350         


Asn Ala Thr Ala Ala Ala Leu Ser Lys Lys Asp Leu Phe Gly Pro Leu 
        355                 360                 365             


Trp Ala Gly Phe Gly Thr Ala Leu Gln Asp Glu Ile Val Thr Gln Leu 
    370                 375                 380                 


Met Thr Glu Glu Ser Glu Pro Arg Leu Ile Ala Trp Leu Gln Glu His 
385                 390                 395                 400 


Thr Gly Val Asp Glu Ala Arg Ala Gln Ala Ile Ala Asn Thr Gly Leu 
                405                 410                 415     


Pro Glu Gly Tyr Gly Ser Leu Gly Arg Thr Ala Leu Ala Arg Ile Val 
            420                 425                 430         


Pro Glu Leu Gln Arg Asp Val Ile Thr Tyr Asp Lys Ala Val Gln Ala 
        435                 440                 445             


Ala Gly Phe Ala His His Ser Asp Leu Gly Phe Ser Phe Glu His Gly 
    450                 455                 460                 


Ala Asp Glu Val Glu Arg Ile Gly Glu Arg Thr Ile Ala Ser Thr Gly 
465                 470                 475                 480 


Glu Ile Lys Pro Val Phe Ala Phe Lys Gln Leu Pro Tyr Tyr Gly Arg 
                485                 490                 495     


Ala Leu Gln Arg His Val Ala Phe Ala Lys Asp Asn Pro Arg Asn Asp 
            500                 505                 510         


Glu Glu Arg Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ile Gly Leu 
        515                 520                 525             


Asn Gln Val Arg Val Val Val Asn Ala Leu Met Arg Arg Tyr Gly Arg 
    530                 535                 540                 


Pro Ala Glu Val Val Ile Glu Leu Ala Arg Asp Leu Lys Gln Ser Arg 
545                 550                 555                 560 


Glu Gln Lys Leu Glu Ala Gln Gln Arg Gln Ala Asp Asn Gln Lys Arg 
                565                 570                 575     


Asn Ala Arg Ile Arg Arg Ser Ile Ala Glu Val Leu Gly Ile Ser Glu 
            580                 585                 590         


Glu Arg Val Arg Thr Leu Asp Ile Gln Lys Trp Ile Leu Trp Glu Glu 
        595                 600                 605             


Leu Ser His Asp Ala Ala Asp Arg Arg Cys Pro Tyr Ser Gly Val Gln 
    610                 615                 620                 


Ile Ser Ala Ser Met Leu Leu Gly Glu Arg Val Glu Ile Glu His Ile 
625                 630                 635                 640 


Leu Pro Phe Ser Met Thr Leu Asp Asp Ser Leu Asn Asn Arg Thr Val 
                645                 650                 655     


Ala Leu Arg Glu Ala Asn Arg Ile Lys Arg Asn Arg Ala Pro Trp Glu 
            660                 665                 670         


Ala Arg Ala Asp Phe Glu Ala Gln Gly Trp Ser Tyr Asp Gly Ile Leu 
        675                 680                 685             


Gln Arg Ala Glu Arg Met Pro Leu Arg Lys Arg Tyr Arg Phe Ala Pro 
    690                 695                 700                 


Asp Gly Tyr Glu Arg Trp Leu Gly Glu Asp Lys Asp Phe Leu Ala Arg 
705                 710                 715                 720 


Ala Leu Asn Asp Thr Arg Tyr Leu Ser Arg Val Ala Ala Glu Tyr Leu 
                725                 730                 735     


Arg Leu Val Cys Pro Gly Ser Ala Thr Arg Val Ile Pro Gly Gln Met 
            740                 745                 750         


Thr Ala Leu Leu Arg Gly Lys Phe Gly Leu Asn Asp Val Leu Gly Leu 
        755                 760                 765             


Asp Gly Glu Lys Asn Arg Asn Asp His Arg His His Ala Val Asp Ala 
    770                 775                 780                 


Cys Val Ile Gly Val Thr Asp Gln Arg Met Met Gln Arg Phe Ala Gln 
785                 790                 795                 800 


Ala Asn Ala Gln Ala Arg Glu Asp Gly Leu Thr Arg Leu Val Glu Asp 
                805                 810                 815     


Met Pro Leu Pro Trp Pro Thr Tyr Arg Asp His Val Glu Arg Ala Val 
            820                 825                 830         


His His Ile Trp Val Ser His Lys Pro Asp His Gly Phe Glu Gly Ala 
        835                 840                 845             


Met Met Glu Glu Thr Ser Tyr Gly Ile Leu Arg Asp Gly Ser Ile Lys 
    850                 855                 860                 


Gln Arg Arg Lys Ala Asp Gly Ser Ala Gly Arg Glu Ile Ser Asn Leu 
865                 870                 875                 880 


Ile Arg Ile Ala Glu Pro Gly Gln Pro Gln Arg His Gly Val Asp Ala 
                885                 890                 895     


Glu Gly Arg Pro Leu Pro Tyr Lys Gly Tyr Val Gly Gly Ser Asn Tyr 
            900                 905                 910         


Cys Ile Glu Ile Thr Val Asn Asp Lys Gly Lys Trp Glu Gly Gln Val 
        915                 920                 925             


Ile Ser Thr Phe Asp Ala Tyr Arg Ile Val Arg Glu Gly Gly Leu Arg 
    930                 935                 940                 


Gln Leu Arg His Pro Thr Gln Gly Gln Asn Gly Arg Ala Leu Ala Met 
945                 950                 955                 960 


Arg Leu Val Ile Gly Asp Ser Val Arg Leu Glu Ala Gly Glu Gly Ser 
                965                 970                 975     


Leu Gln Thr Leu Arg Val Ala Lys Met Ser Ala Ala Gly Gln Val Phe 
            980                 985                 990         


Met Ser Glu Val Asn Gln Ala Asn  Val Asp Ala Arg Asn  Arg Asn Gly 
        995                 1000                 1005             


Asp Leu  Pro Tyr Val Ser Lys  Met Ala Gly Ser Phe  Gln Lys Ala 
    1010                 1015                 1020             


Arg Ala  Arg Gln Val Thr Ile  Ser Pro Ile Gly Glu  Leu Arg Asp 
    1025                 1030                 1035             


Pro Gly  Phe Lys Gly 
    1040             


<210>  507
<211>  1044
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  507

Met Lys Tyr Thr Leu Gly Leu Asp Leu Gly Tyr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Ile Leu Glu Asn Asp Glu Asn Gly Ser Pro Ile Lys Leu Phe Asn 
            20                  25                  30          


Ala Gly Val His Ile Phe Glu Pro Ser Ile Glu Asp Ile Glu Ile Asp 
        35                  40                  45              


Gly Lys Gly Lys Thr Lys Asn Val Lys Arg Arg Glu Ala Arg Leu Arg 
    50                  55                  60                  


Arg Arg Gln Leu Phe Arg Lys Thr Lys Arg Leu Glu Lys Leu Leu Asn 
65                  70                  75                  80  


Phe Phe Ile Gln Glu Gly Phe Phe Gln Asn Gln Lys Val Leu Ile Asn 
                85                  90                  95      


Asp Pro Phe Glu Arg Ala Glu Phe Leu Tyr Gln Leu Asp Lys Lys Ser 
            100                 105                 110         


Arg Asn Pro Tyr Ile Leu Arg Arg Glu Ala Leu Asp Asn Arg Leu Ser 
        115                 120                 125             


Leu Glu Glu Ile Ala Arg Ile Phe Tyr His Ile Ala Gln His Arg Gly 
    130                 135                 140                 


Phe Lys Ser Thr Lys Arg Glu Leu Ala Glu Met Asn Lys Asp Glu Lys 
145                 150                 155                 160 


Lys Lys Lys Glu Asp Gly Ile Ile Gln Ser Glu Ile Asn Thr Leu Lys 
                165                 170                 175     


Glu Glu Ile Lys Lys Asn Ser Cys Arg Thr Leu Gly Glu Tyr Phe Ala 
            180                 185                 190         


Lys Leu Phe Glu Asn Gly Glu Lys Ile Arg Gly Arg His Thr Asp Arg 
        195                 200                 205             


Lys Met Tyr Glu Glu Glu Phe Gln Lys Ile Phe Asn Lys Gln Lys Glu 
    210                 215                 220                 


Phe Tyr Pro Asn Ile Phe Thr Asp Glu Phe Lys Lys Lys Leu Tyr Lys 
225                 230                 235                 240 


Leu Ile Phe His Gln Arg Glu Leu Lys Ser Gln Lys Asp Lys Val Gly 
                245                 250                 255     


Phe Cys Ser Leu Glu Arg Gly Lys Lys Arg Ala Pro Leu Ala Leu Leu 
            260                 265                 270         


Val Ser Gln Arg Tyr Arg Tyr Met Cys Lys Val Asn Asn Leu Asn Val 
        275                 280                 285             


Ile Asp Thr Glu Thr Gly Glu Asn Arg Glu Leu Thr Val Asp Glu Lys 
    290                 295                 300                 


Lys Lys Leu Tyr Glu Lys Leu Glu Asn Ser Glu Lys Leu Ser Phe Lys 
305                 310                 315                 320 


Glu Ile Lys Lys Ile Leu Asn Leu Ser Glu Lys Arg Tyr Ile Phe Asn 
                325                 330                 335     


Leu Glu Lys Ser Glu Lys Tyr Ile Ile Gly Asn Lys Thr Asn Val Lys 
            340                 345                 350         


Met Lys Asn Ile Ile Lys Asp Trp Asp Asn Phe Asp Glu Asp Lys Lys 
        355                 360                 365             


Asn Phe Phe Ile Asn Glu Ala Arg Thr Val Val Lys Ile Glu Thr Leu 
    370                 375                 380                 


Glu Arg Arg Gly Lys Lys Leu Gly Leu Ser Asp Glu Glu Ser Lys Glu 
385                 390                 395                 400 


Phe Ser Lys Val Val Phe Glu Asp Gly Tyr Leu Asn Phe Ser Lys Lys 
                405                 410                 415     


Ala Val Glu Lys Leu Leu Pro Tyr Leu Lys Glu Gly Lys Lys Glu Tyr 
            420                 425                 430         


Glu Ala Ile Lys Leu Ala Tyr Pro Glu Asn Ser Val Gln Asn Asp Asn 
        435                 440                 445             


Leu Lys Glu Leu Asp Phe Leu Pro Pro Ile Lys Ser Lys Glu Leu Lys 
    450                 455                 460                 


Lys Tyr Phe Glu Val Arg Asn Pro Ile Val Glu Lys Ala Leu Thr Glu 
465                 470                 475                 480 


Thr Arg Tyr Leu Val Asn Phe Leu Ile Lys Lys Tyr Gly Lys Pro Glu 
                485                 490                 495     


Thr Ile Lys Ile Glu Leu Ala Arg Glu Leu Lys Leu Ser Asn Lys Gln 
            500                 505                 510         


Lys Glu Arg Lys His Lys Glu Asn Gln Glu Asn Arg Lys Lys Arg Glu 
        515                 520                 525             


Glu Val Ile Glu Lys Leu Lys Asn Asp Leu Lys Ile Ser Glu Pro Thr 
    530                 535                 540                 


Glu Thr Gln Ile Gln Lys Tyr Leu Leu Ala Glu Glu Cys Asp Cys Lys 
545                 550                 555                 560 


Cys Pro Tyr Thr Gly Arg Ser Ile Ser Met Asp Ser Leu Phe Gly Glu 
                565                 570                 575     


His Pro Leu Phe Asp Ile Glu His Ile Ile Pro Phe Asp Arg Ser Leu 
            580                 585                 590         


Asp Asn Ser Phe Leu Asn Lys Thr Leu Cys Tyr Ser Glu Glu Asn Arg 
        595                 600                 605             


Lys Val Lys His Asn Lys Thr Pro Tyr Glu Ala Tyr Tyr Gly Thr Glu 
    610                 615                 620                 


Lys Trp Glu Ala Ile Ile Glu Arg Val Lys Lys Phe Lys Ser Asn Val 
625                 630                 635                 640 


Arg Asn Glu Lys Leu Arg Arg Phe Gln Met Thr Lys Glu Asp Val Glu 
                645                 650                 655     


Asn Tyr Ile Gln Asn Phe Ser Thr Gln Asn Leu Asn Asp Thr Lys Tyr 
            660                 665                 670         


Ala Ser Lys Leu Ala Lys Lys Tyr Leu Gly Leu Leu Tyr Gly Ser Ile 
        675                 680                 685             


Asn Glu Asp Gly Ile Asp Lys Asn Arg Lys Arg Arg Ile Phe Ala Ser 
    690                 695                 700                 


Ser Gly Lys Val Thr Ser Thr Leu Arg Tyr Ala Leu Gly Leu Asn Thr 
705                 710                 715                 720 


Ile Leu Asn Asp Glu Asn Ile Lys Thr Arg Glu Asp His Arg His His 
                725                 730                 735     


Ala Ile Asp Ala Ile Thr Ile Ala Leu Thr Asp Ser Ser Met Ile Lys 
            740                 745                 750         


Lys Ile Ser Asp Ala Ser Lys Arg Glu Lys Asn Ile Asn Ser Leu Phe 
        755                 760                 765             


Lys Asp Phe Gln Ala Pro Trp Glu Asn Phe Arg Lys Glu Val Glu Glu 
    770                 775                 780                 


Ile Ile Ala Lys Met Asn Ile Ser Ile Arg Ile Asn Lys Lys Val Ser 
785                 790                 795                 800 


Gly Gln Ile His Asp Glu Thr Phe Tyr Lys Lys Pro Phe Lys Glu Glu 
                805                 810                 815     


Glu Lys Ser Tyr Thr His Gln Arg Ile Asn Leu Glu Glu Leu Thr Ile 
            820                 825                 830         


Lys Asp Glu Asp Lys Lys Lys Gln Ser Ser Lys Leu Ile Asp Asn Ile 
        835                 840                 845             


Val Asp Ser Lys Ile Arg Asn Ile Ile Ile Asn Ser Leu Asn Gly Glu 
    850                 855                 860                 


Lys Pro Lys Ile Arg Phe Lys Asp Pro Lys Asp His Pro Tyr Ile Ile 
865                 870                 875                 880 


Gln Lys Asn Gly Lys Lys Ile Gln Ile His Lys Val Thr Ile Lys Thr 
                885                 890                 895     


Lys Leu Glu Thr Tyr Asn Glu Ile Gly Pro Glu Lys Ala Lys Arg Tyr 
            900                 905                 910         


Val Lys Ser Ser Asp Ser His His Ile Glu Ile Phe Glu Asp Leu Lys 
        915                 920                 925             


Thr Lys Lys Trp Asp Gly Tyr Val Val Ser Leu Leu Glu Ala Tyr Glu 
    930                 935                 940                 


Arg Lys Lys Lys Lys Leu Pro Ile Val Asn Lys Asn Leu Gly Glu Gly 
945                 950                 955                 960 


Lys Lys Phe Leu Phe Ser Leu Ala Lys Gly Glu Ile Ile Glu Leu Asp 
                965                 970                 975     


Thr Glu Asn Gly Lys Arg Glu Leu Phe Val Val Arg Ile Ile Ser Ala 
            980                 985                 990         


Ser Asn Asn Gln Ile Lys Phe Thr  Arg Ile Asn Asp Ser  Arg Lys Ile 
        995                 1000                 1005             


Thr Asp  Ile Pro Ala Thr Gly  Leu Thr Ala Cys Pro  Ser Ser Leu 
    1010                 1015                 1020             


Lys Lys  Arg Asn Cys Lys Lys  Val Val Ile Asn Ile  Leu Gly Glu 
    1025                 1030                 1035             


Lys His  Tyr Ser Asn Asp 
    1040                 


<210>  508
<211>  1044
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  508

Met Ala Glu Lys Lys Phe Lys Tyr Arg Leu Gly Leu Asp Leu Gly Thr 
1               5                   10                  15      


Asn Ser Ile Gly Trp Cys Met Leu Arg Leu Asn Lys Asp Asn Glu Pro 
            20                  25                  30          


Ala Ala Val Ile Lys Ser Gly Val Arg Ile Phe His Asp Gly Arg Asn 
        35                  40                  45              


Glu Lys Thr Lys Glu Pro Leu Ala Val Val Arg Arg Asn Ala Arg Ser 
    50                  55                  60                  


Ile Arg Arg Asn Leu Asp Arg Lys Ile Ser Arg Arg Asn His Leu Leu 
65                  70                  75                  80  


Asn Thr Leu Val Lys Tyr Phe Leu Leu Pro Ser Asn Glu Ile Glu Arg 
                85                  90                  95      


Lys Ala Leu Ala Val Leu Asn Pro Tyr Glu Leu Arg Ser Arg Ala Val 
            100                 105                 110         


Asn Glu Arg Leu Glu Leu Phe Glu Leu Gly Arg Leu Leu Met His Leu 
        115                 120                 125             


Ser Lys Arg Arg Gly Phe Lys Ser Asn Arg Lys Val Asp Lys Glu Asp 
    130                 135                 140                 


Ser Ser Gly Lys Ile Lys Pro Ala Ile Glu Arg Leu Lys Asn Lys Met 
145                 150                 155                 160 


Gln Glu Thr Asn Lys Lys Thr Ala Gly Glu Tyr Leu Tyr Ser Leu Met 
                165                 170                 175     


Gln Asn Gly Lys Pro Val Arg Ala Arg Leu Gly Arg Ile Asp Gly Ser 
            180                 185                 190         


Asn Gly Tyr Glu Ile Tyr Leu Asp Arg Ala Leu Ile Glu Glu Glu Tyr 
        195                 200                 205             


Asn Leu Ile Met Asn Glu Gln Lys Lys His His Lys Glu Leu Thr Asp 
    210                 215                 220                 


Asp Ile Ile Asn Glu Ile Phe Asn Ile Ile Phe Tyr Gln Arg Pro Leu 
225                 230                 235                 240 


Lys Lys Gln Ile Ile Gly Lys Cys Ser Leu Thr Gly Glu Asp Thr Lys 
                245                 250                 255     


Leu Lys Lys Ala His Val Leu Ala Gln Asp Phe Ile Leu Leu Gln Lys 
            260                 265                 270         


Val Gln Asp Leu Arg Val Phe Asp Asp Asn Gln Leu Glu Phe Arg Pro 
        275                 280                 285             


Leu Ser Asn Asp Glu Lys Asp Ile Ile Lys Arg Glu Leu Arg Lys Lys 
    290                 295                 300                 


Lys Glu Val Ser Tyr Glu Asn Ile Arg Lys Leu Leu Lys Lys Tyr Tyr 
305                 310                 315                 320 


Glu Asn Val Lys Tyr Gly Met Phe Ser His Glu Thr Leu Tyr Lys Lys 
                325                 330                 335     


Leu Gln Gly Asn Lys Thr Asn Ala Leu Met Ser Asp Lys Glu Ile Ile 
            340                 345                 350         


Gly Asp Ser Trp Tyr Ser Leu Ser Pro Glu Val Gln Tyr Asp Ile Ile 
        355                 360                 365             


Glu Glu Leu Tyr Ser Asp Lys Asp Asn Asp Glu Leu Ser Lys Leu Tyr 
    370                 375                 380                 


Ser Glu Lys Phe Asn Leu Asn Glu Ser Gln Ile Asn Gly Leu Leu Asn 
385                 390                 395                 400 


Lys Ala Ile Tyr Arg Leu Asp Ser Gly Tyr Ile Arg Tyr Gly Lys Thr 
                405                 410                 415     


Ala Val Thr Asn Leu Asn Ser Ile Met Asp Lys Asp Asn Leu Asn Leu 
            420                 425                 430         


His Asp Ala Arg Glu Glu Ala Gly Tyr Glu Lys Glu Asn Lys Gln Asp 
        435                 440                 445             


Ala Ser Glu Tyr Leu Glu Tyr Tyr Gly Lys Val Leu Pro Asp Ser Val 
    450                 455                 460                 


Val Asp Pro Gln Ile Glu Asn Pro Lys Asn Asp Glu Glu Arg Tyr Gly 
465                 470                 475                 480 


Lys Ile Ala Asn Pro Thr Val His Ala Ala Leu Asn Gln Leu Arg Lys 
                485                 490                 495     


Leu Val Asn Glu Leu Ile Lys Leu Tyr Gly Lys Pro Glu Gln Ile Val 
            500                 505                 510         


Ile Glu Leu Ala Arg Asp Leu Lys Asn Ser Arg Lys Ala Lys Asp Val 
        515                 520                 525             


Ile Asn Lys Lys Ile Ala Glu Asn Arg Lys Leu Asn Glu Glu Val Asp 
    530                 535                 540                 


Lys Thr Leu Gln Glu Tyr Ser Glu Lys Tyr Asn Thr His Ile Val Lys 
545                 550                 555                 560 


Asn Lys Leu Asn Arg Asp Lys Val Lys Leu Trp Arg Glu Leu Gly Lys 
                565                 570                 575     


Asp Pro Leu Glu Arg Lys Cys Ile Tyr Thr Gly Glu Gln Ile Asn Ile 
            580                 585                 590         


Ala Lys Leu Phe Ser Asp Glu Val Gln Ile Glu His Ile Val Pro Tyr 
        595                 600                 605             


Ser Lys Thr Leu Asp Asp Ser Phe Asn Asn Lys Thr Leu Ser Met Lys 
    610                 615                 620                 


Gln Ala Asn Tyr Tyr Lys Gly Asn Lys Thr Pro Tyr Glu Ala Phe Lys 
625                 630                 635                 640 


Asp Ser Lys Asp Gly Tyr Asn Tyr Asp Asp Ile Ile Ala Arg Ala Lys 
                645                 650                 655     


Leu Leu Asn Asn Asp Asn Lys Phe Lys Arg Phe Thr Lys Asp Ala Met 
            660                 665                 670         


Asp Ile Tyr Asn Arg Asp Gly Lys Asp Phe Ile Ala Arg Gln Leu Ser 
        675                 680                 685             


Asp Thr Gln Tyr Leu Ser Thr Arg Ala Leu Ile Tyr Leu Gln Ser Leu 
    690                 695                 700                 


Tyr Glu Lys Ala His His Ser Ser Ile Trp Thr Ile Pro Gly Gln Leu 
705                 710                 715                 720 


Thr Ala Leu Ile Arg Asn Tyr Leu Gly Leu Asn Thr Leu Ile Ser Asn 
                725                 730                 735     


Asp Lys Asn Lys Asn Arg Asn Asp His Arg His His Ala Val Asp Ala 
            740                 745                 750         


Phe Val Val Ala Val Thr Ser Arg Ser Leu Leu Gln Lys Ile Ser Tyr 
        755                 760                 765             


Ala Ala Ser Leu Gln Glu Asp Leu Asp Asn Arg Asn Val Ser Ser Tyr 
    770                 775                 780                 


Arg Asn Lys Leu Leu Glu Lys Met Pro Glu Pro Phe Pro Asn Tyr Arg 
785                 790                 795                 800 


Lys Ser Leu Glu Leu Ala Ile Asp Asn Ile Lys Thr Ser His Lys Ala 
                805                 810                 815     


Asp Arg Ser Ile Ser Gly Lys Leu His Glu Asp Thr Ala Tyr Gly Ile 
            820                 825                 830         


Val Glu Asn Asp Asp Lys Tyr Asn Val Val Thr Arg Trp Thr Val Asp 
        835                 840                 845             


Lys Phe Lys Glu Gln Lys His Phe Glu Thr Ile Arg Asp Asn Glu Leu 
    850                 855                 860                 


Lys Glu Ile Ala Cys Ser Asp Lys Glu Leu Phe Ala Lys Ile Val Glu 
865                 870                 875                 880 


Thr Arg Lys Ile Lys Ser Ile Arg Lys Leu Ala Lys Glu Asn Pro Val 
                885                 890                 895     


Ile Lys Ile Lys Asp Lys Ser Gly Arg Asp Tyr Lys Ala Phe Ala Gly 
            900                 905                 910         


Gly Asn Asn Ile Cys Val Glu Ile Tyr Glu Val Asp Gly Val Arg Lys 
        915                 920                 925             


Ser Glu Val Ile Gln Leu Phe Asp Ala Ala Gln Lys Gly Phe Met Pro 
    930                 935                 940                 


Glu Trp Met Lys Lys Tyr Pro Asn Gly Lys Leu Leu Met Arg Leu Phe 
945                 950                 955                 960 


Lys Gly Asp Val Ile Gly Phe Ile Glu Asn Arg Lys Tyr Asn Tyr Tyr 
                965                 970                 975     


Thr Ile Lys Gly Ile Ser Ile Ala Asp Gln Asn Leu Lys Leu Leu Ala 
            980                 985                 990         


Ile Asn Lys Ala Glu Gly Ile Phe  Lys Lys Gly Leu Asn  Ile Leu Phe 
        995                 1000                 1005             


Asp Ser  Asn Asn Lys Asn Ser  Leu Asn Ala Lys Gln  Phe Asn Ile 
    1010                 1015                 1020             


Ser Val  Thr Gly Ile Val Thr  Lys Lys Lys Thr Ala  Asp Val Asn 
    1025                 1030                 1035             


Phe Trp  Lys Asn Arg Lys 
    1040                 


<210>  509
<211>  1044
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  509

Met Leu His Lys Met Arg Tyr Arg Leu Ala Leu Asp Leu Gly Ser Thr 
1               5                   10                  15      


Ser Leu Gly Trp Ala Met Val Arg Leu Asn Ala Asn Gln Gln Pro Cys 
            20                  25                  30          


Ala Val Ile Lys Ala Gly Val Arg Ile Phe Ser Asn Gly Arg Asn Pro 
        35                  40                  45              


Lys Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Glu Ala Arg Ser Met 
    50                  55                  60                  


Arg Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Ala Arg Met Met Arg 
65                  70                  75                  80  


Thr Leu Ile Glu Tyr Gly Phe Phe Pro Ala Asn Glu Thr Gln Arg Lys 
                85                  90                  95      


Ala Leu Glu Asn Leu Asn Pro Tyr Ala Leu Arg Ala Lys Gly Leu Asp 
            100                 105                 110         


Glu Ala Leu Thr Pro Ser Glu Phe Ala Arg Ala Leu Phe His Ile Asn 
        115                 120                 125             


Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Lys Asp Ser 
    130                 135                 140                 


Asp Ser Gly Ala Leu Lys Thr Ala Ile Lys Gln Leu His Ser Val Leu 
145                 150                 155                 160 


Asp Pro Gln Gly Asn Asp Gly Lys Pro Arg Thr Val Gly Glu Leu Leu 
                165                 170                 175     


Tyr Lys Arg Phe Thr Asp Leu Ser Lys Leu Pro Lys Asp Arg Thr Val 
            180                 185                 190         


Arg Ala Arg Tyr Arg Gln Asp Lys Ile Val Lys Asp Asp Gly Lys Thr 
        195                 200                 205             


Lys Ile Asp Lys Tyr Tyr Asp Leu Tyr Ile Asp Arg Ala Met Ile Glu 
    210                 215                 220                 


Gln Glu Phe Asp Ala Leu Trp Lys Lys Gln Ser Glu Leu Asn Pro Ile 
225                 230                 235                 240 


Leu Phe Thr Glu Asn Ala Arg Ala Asp Leu Lys Asp Val Leu Leu Tyr 
                245                 250                 255     


Gln Arg Ser Leu Lys Pro Val Lys Pro Gly Arg Cys Thr Phe Met Ser 
            260                 265                 270         


Glu Glu Glu Arg Ala Pro Leu Ala Leu Pro Ser Thr Gln Arg Phe Arg 
        275                 280                 285             


Met Tyr Gln Glu Val Asn Asn Leu Arg Ile Leu Arg Glu Gly Leu Lys 
    290                 295                 300                 


Glu Glu Ser Leu Thr Leu Gln Gln Arg Asp Asp Leu Ile Asn Ala Leu 
305                 310                 315                 320 


Glu Lys Asn Asn Arg Arg Thr Phe Thr Gln Ile Lys Lys Leu Leu Gly 
                325                 330                 335     


Val Gly Gly Ser Val Gln Phe Asn Phe Glu Asp Pro Lys Arg Glu Glu 
            340                 345                 350         


Leu Lys Gly Asn Thr Thr Ser Ala Ile Leu Gly Lys Ser Glu His Phe 
        355                 360                 365             


Gly Glu Ala Trp Phe Ala Phe Asn Glu Ala Lys Gln Asp Ala Ile Val 
    370                 375                 380                 


Leu Gln Leu Ile Lys Glu Glu Asn Glu Ala Lys Leu Val Arg Trp Leu 
385                 390                 395                 400 


Gln Asp Glu Thr Gly Ile Asp Glu Lys Arg Ala Glu Val Ile Ala Asn 
                405                 410                 415     


Ala Gly Leu Pro Glu Gly Tyr Gly Ser Leu Cys Ile Glu Ala Leu Ala 
            420                 425                 430         


Arg Ile Leu Pro Glu Leu Arg Arg Asp Val Val Thr Tyr Asp Lys Ala 
        435                 440                 445             


Val Gln Ala Ala Gly Phe Glu His His Ser Lys Leu Asn Arg Asn Glu 
    450                 455                 460                 


Glu Ile Pro Asp Ile Thr Phe Lys Ile Glu Ser Ile Asp Arg Asn Ser 
465                 470                 475                 480 


Gly Glu Ile Lys Gln Phe His Leu His Lys Glu Leu Pro Tyr Tyr Gly 
                485                 490                 495     


Glu Tyr Leu Gln Arg His Val Gly Phe Gly Ser Gly Lys Pro Glu Asp 
            500                 505                 510         


Ser Ile Glu Lys Arg Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ile 
        515                 520                 525             


Gly Leu Asn Gln Val Arg Val Val Val Asn Ala Leu Ile Lys Arg Tyr 
    530                 535                 540                 


Gly His Pro Ser Glu Ile Ile Val Glu Val Ala Arg Asp Leu Lys Gln 
545                 550                 555                 560 


Ser Lys Asp Arg Arg Asp Glu Glu Asn Lys Arg Gln Ala Glu Asn Gln 
                565                 570                 575     


Lys Arg Asn Glu Arg Leu Arg Lys Asp Ile Ala Asp Ile Leu Gly Ile 
            580                 585                 590         


Ser Glu Glu Arg Val Arg Arg Asp Asp Ile Glu Lys Met Ile Leu Trp 
        595                 600                 605             


Glu Glu Leu Ser Phe Asp Pro Ala Asp Arg Arg Cys Pro Tyr Ser Gly 
    610                 615                 620                 


Val Gln Val Ser Thr Val Met Leu Leu Ser Asp Glu Val Glu Val Glu 
625                 630                 635                 640 


His Ile Leu Pro Phe Ser Gln Thr Leu Asp Asp Ser Leu Asn Asn Lys 
                645                 650                 655     


Thr Val Ala Leu Arg Gln Ala Asn Arg Ile Lys Gly Asn Arg Thr Pro 
            660                 665                 670         


Trp Glu Ala Phe Gly Thr Ser Asn Ile Ser Gly Phe Asp Tyr Ser Gly 
        675                 680                 685             


Ile Leu Thr Arg Ala Glu Leu Met Pro Lys Ala Lys Arg Tyr Arg Phe 
    690                 695                 700                 


Ala Glu Asp Gly Tyr Gln Arg Trp Leu Lys Asp Asp Ala Gly Phe Leu 
705                 710                 715                 720 


Ala Arg Ala Leu Asn Asp Thr Arg His Leu Ser Lys Ile Ala Arg Glu 
                725                 730                 735     


Tyr Met Ser Leu Ile Cys Pro Asn Thr Arg Val Ile Pro Gly Gln Met 
            740                 745                 750         


Thr Ala Met Leu Arg Arg Asn Phe Gly Leu Asn Asp Val Leu Gly Leu 
        755                 760                 765             


Asn Gly Glu Lys Asn Arg Asn Asp His Arg His His Ala Val Asp Ala 
    770                 775                 780                 


Cys Val Ile Ala Val Thr Asp Gln Gly Leu Leu Gln Arg Phe Ala Ala 
785                 790                 795                 800 


Ala Ser Ala Ser Ala Arg Glu Arg Gln Leu Asn Arg Leu Val Glu Asn 
                805                 810                 815     


Met Pro Leu Pro Trp Glu Ser Tyr Arg Glu His Val Arg Arg Ala Ile 
            820                 825                 830         


Asp Gly Ile Trp Val Ser His Arg Pro Asp His Ser His Glu Gly Ala 
        835                 840                 845             


Met His Asn Asp Thr Ala Tyr Gly Leu Arg Gly Asn Gly Arg Val Ser 
    850                 855                 860                 


Phe His Lys Met Val Glu Gly Lys Arg Glu Tyr Ile Glu Asp Asn Leu 
865                 870                 875                 880 


Lys Val Ile Glu Ile Thr Asp Thr Lys Ala Ile Asp Arg His Gly Leu 
                885                 890                 895     


Leu Pro Thr Gly Lys Pro Arg Pro Tyr Lys Gly Tyr Lys Gly Asp Ser 
            900                 905                 910         


Asn Tyr Cys Ile Glu Ile Val Arg Asn Glu Lys Gly Arg Trp Glu Gly 
        915                 920                 925             


Glu Ile Ile Ser Thr Phe Asp Ala Tyr Gln Leu Val Arg Lys Tyr Gly 
    930                 935                 940                 


Ile Val Gln Leu Arg Tyr Pro Val Leu Ser Val Ser Gly Lys Pro Leu 
945                 950                 955                 960 


Val Met Arg Leu Ile Arg Asn Asp Phe Val Arg Leu Glu His Glu Gly 
                965                 970                 975     


Ile Thr Arg Thr Leu Arg Val Cys Lys Met Arg Glu Asp Gly Gln Val 
            980                 985                 990         


Ala Leu Ala Asp Val Thr Glu Ala  Asn Val Asp Ala Arg  Thr Arg Thr 
        995                 1000                 1005             


Lys Glu  Ile Cys Tyr Val Phe  Lys Thr Gly Gly Ser  Leu Gln Lys 
    1010                 1015                 1020             


Ala Lys  Ala Arg Arg Ile Thr  Ile Ser Pro Ile Gly  Glu Leu Arg 
    1025                 1030                 1035             


Asp Pro  Gly Phe Lys Glu 
    1040                 


<210>  510
<211>  1045
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  510

Met Gln Lys Glu Lys Pro Leu Ala Tyr Arg Leu Ala Leu Asp Leu Gly 
1               5                   10                  15      


Ser Thr Ser Leu Gly Trp Ala Val Leu Arg Leu Ser Ala Pro Gly Glu 
            20                  25                  30          


Pro Ser Ala Val Ile Arg Ala Gly Ser Arg Ile Phe Ser Asp Gly Arg 
        35                  40                  45              


Asn Pro Lys Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Ala Ala Arg 
    50                  55                  60                  


Ala Met Arg Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Ala Arg Met 
65                  70                  75                  80  


Leu Ala Ala Leu Glu Arg His Gly Phe Phe Pro Gln Asp Glu Ala Ala 
                85                  90                  95      


Arg Lys Ala Leu Glu Lys Leu Asn Pro Tyr Gly Leu Arg Ala Arg Gly 
            100                 105                 110         


Leu Glu Ser Ala Leu Lys Pro Glu Glu Phe Gly Arg Ala Leu Phe His 
        115                 120                 125             


Leu Asn Gln Arg Arg Gly Phe Gln Ser Asn Arg Lys Thr Asp Arg Lys 
    130                 135                 140                 


Asp Ser Asp Ser Gly Ala Leu Lys Gln Ala Ile Gly Thr Leu Arg Ala 
145                 150                 155                 160 


Gln Met His Glu Ser Gly Cys Arg Thr Val Gly Glu Trp Phe Trp Lys 
                165                 170                 175     


Val Arg Met Glu Arg Lys Pro Glu Gly Val Lys Gly Gln Gly Val Arg 
            180                 185                 190         


Ala Arg Tyr Arg Glu Thr Pro Phe Thr Thr Asp Glu Gly Lys Lys Arg 
        195                 200                 205             


Ile Asp Lys Arg Tyr Asp Leu Tyr Val Asp Arg Ala Met Val Ala Gln 
    210                 215                 220                 


Glu Phe Asp Ala Leu Trp Ala Ala Gln Ala Thr Phe Ala Pro Ala Leu 
225                 230                 235                 240 


Phe Asn Glu Ala Ala Arg Ala Glu Leu Arg Asp Ile Leu Leu His Gln 
                245                 250                 255     


Arg Lys Leu Arg Pro Val Gln Pro Gly Arg Cys Thr Leu Val Pro Glu 
            260                 265                 270         


Glu Pro Arg Ala Leu Leu Ala Leu Pro Ser Thr Gln Arg Phe Arg Ile 
        275                 280                 285             


Tyr Gln Glu Val Asn His Leu Arg Val Leu His Asp Glu Leu Arg Glu 
    290                 295                 300                 


Glu Ala Leu Thr Leu Ala Gln Arg Asp Gln Ile Val Ala Leu Leu Glu 
305                 310                 315                 320 


Arg Gln Ala Lys Val Thr Phe Thr Ala Met Arg Lys Ala Leu Gly Phe 
                325                 330                 335     


Ser Ala Leu Phe Asn Leu Glu Asp Ala Lys Arg Asp Gly Leu Lys Gly 
            340                 345                 350         


Asn Ala Thr Thr Ala Ala Leu Gly Lys Lys Asp Leu Phe Gly Pro Leu 
        355                 360                 365             


Trp Ala Gly Phe Asp Thr Ala Leu Gln Asp Glu Ile Val Thr Gln Leu 
    370                 375                 380                 


Met Thr Glu Glu Ser Glu Pro Arg Leu Ile Ala Trp Leu Gln Glu His 
385                 390                 395                 400 


Thr Gly Val Asp Glu Ala Arg Ala Gln Ala Ile Ala Asn Ala Gly Leu 
                405                 410                 415     


Pro Glu Gly Tyr Gly Ser Leu Gly Arg Thr Ala Leu Ala Arg Ile Val 
            420                 425                 430         


Pro Glu Leu Gln Arg Asp Val Ile Thr Tyr Asp Lys Ala Val Gln Ala 
        435                 440                 445             


Ala Gly Phe Ala His His Ser Asp Leu Gly Phe Ser Phe Glu His Asp 
    450                 455                 460                 


Ala Asp Glu Val Glu Arg Ile Gly Glu Arg Thr Ile Ala Ser Thr Gly 
465                 470                 475                 480 


Glu Ile Lys Pro Val Phe Ala Phe Lys Gln Leu Pro Tyr Tyr Gly Arg 
                485                 490                 495     


Ala Leu Gln Arg His Val Ala Phe Ala Lys Glu Asn Pro Arg Asn Asp 
            500                 505                 510         


Glu Glu Arg Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ile Gly Leu 
        515                 520                 525             


Asn Gln Val Arg Val Val Val Asn Ala Leu Met Cys Arg Tyr Gly Arg 
    530                 535                 540                 


Pro Thr Glu Val Val Ile Glu Leu Ala Arg Asp Leu Lys Gln Ser Arg 
545                 550                 555                 560 


Glu Gln Lys Leu Glu Ala Gln Gln Arg Gln Ala Asp Asn Gln Lys Arg 
                565                 570                 575     


Asn Ala Arg Ile Arg Arg Ser Ile Ala Glu Val Leu Gly Ile Ser Glu 
            580                 585                 590         


Glu Arg Val Arg Thr Leu Asp Ile Gln Lys Trp Ile Leu Trp Glu Glu 
        595                 600                 605             


Leu Ser His Asp Ala Ala Asp Arg Arg Cys Pro Tyr Ser Gly Val Gln 
    610                 615                 620                 


Ile Ser Ala Ser Met Leu Leu Gly Glu Arg Val Glu Ile Glu His Ile 
625                 630                 635                 640 


Leu Pro Phe Ser Met Thr Leu Asp Asp Ser Leu Asn Asn Arg Thr Val 
                645                 650                 655     


Ala Leu Arg Glu Ala Asn Arg Ile Lys Arg Asn Arg Thr Pro Trp Asp 
            660                 665                 670         


Ala Arg Thr Asp Phe Glu Ala His Gly Trp Ser Tyr Asp Gly Ile Leu 
        675                 680                 685             


Gln Arg Ala Glu Arg Met Pro Gln Arg Lys Arg His Arg Phe Ala Pro 
    690                 695                 700                 


Asp Gly Tyr Glu Arg Trp Leu Gly Glu Asp Lys Asp Phe Leu Ala Arg 
705                 710                 715                 720 


Ala Leu Asn Asp Thr Arg Tyr Leu Ser Arg Val Ala Ala Glu Tyr Leu 
                725                 730                 735     


Arg Leu Val Cys Pro Gly Ser Ala Thr Arg Val Ile Pro Gly Gln Met 
            740                 745                 750         


Thr Ala Leu Leu Arg Gly Lys Phe Gly Leu Asn Asp Val Leu Gly Leu 
        755                 760                 765             


Asp Gly Glu Lys Asn Arg Asn Asp His Arg His His Ala Val Asp Ala 
    770                 775                 780                 


Cys Val Ile Gly Val Thr Asp Gln Arg Met Met Gln Arg Phe Ala Gln 
785                 790                 795                 800 


Ala Asn Ala Gln Ala Arg Glu Asp Gly Leu Thr Arg Leu Val Glu Asp 
                805                 810                 815     


Met Pro Leu Pro Trp Pro Thr Tyr Arg Ala His Val Glu Arg Ala Val 
            820                 825                 830         


Arg His Ile Trp Val Ser His Lys Pro Asp His Gly Phe Glu Gly Ala 
        835                 840                 845             


Met Met Glu Glu Thr Ser Tyr Gly Ile Leu Arg Asp Gly Thr Ile Lys 
    850                 855                 860                 


Gln Arg Arg Lys Ala Asp Gly Ser Ala Gly Arg Glu Ile Ser Arg Leu 
865                 870                 875                 880 


Ile Arg Ile Thr Glu Pro Ser Gln Pro Gln Arg His Gly Val Asp Ala 
                885                 890                 895     


Glu Gly Arg Pro Leu Pro Tyr Lys Gly Tyr Val Gly Gly Ser Asn Tyr 
            900                 905                 910         


Cys Leu Glu Ile Thr Val Asn Asp Lys Gly Lys Trp Glu Gly Gln Val 
        915                 920                 925             


Ile Ser Thr Phe Asp Ala Tyr Arg Ile Val Arg Glu Gly Gly Leu Arg 
    930                 935                 940                 


Gln Leu Arg His Ser Thr Gln Gly Gln Asn Gly Gln Ala Leu Ala Met 
945                 950                 955                 960 


Arg Leu Val Ile Gly Asp Ser Val Arg Leu Glu Val Asp Glu Gln Glu 
                965                 970                 975     


Arg Val Met Arg Ile Val Lys Ile Asn Gly Asn Asn Gly Gln Val Phe 
            980                 985                 990         


Met Ala Pro Val His Glu Ala Asn  Val Asp Ala Arg Asn  Ser Asp Lys 
        995                 1000                 1005             


Asn Glu  Ala Phe Ala Tyr Thr  Ser Lys Ile Ala Gly  Ser Phe Gln 
    1010                 1015                 1020             


Lys Thr  Lys Ala Arg Gln Val  Thr Ile Ser Pro Ile  Gly Glu Leu 
    1025                 1030                 1035             


Arg Asp  Pro Gly Phe Lys Gly  
    1040                 1045 


<210>  511
<211>  1045
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  511

Met Lys Pro Arg Asn Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ser Val Val Glu Ile Asp Glu Asn Glu Tyr Pro 
            20                  25                  30          


Ile Arg Leu Ile Asp Val Gly Val Arg Thr Phe Glu Arg Ala Glu Val 
        35                  40                  45              


Pro Lys Thr Gly Glu Ser Leu Ala Leu Ala Arg Arg Leu Ala Arg Ser 
    50                  55                  60                  


Thr Arg Arg Leu Ile Arg Arg Arg Ala Phe Arg Leu Leu Lys Ala Lys 
65                  70                  75                  80  


Arg Leu Leu Lys His His Gln Ile Val Asn Ala Glu Glu Leu Thr Gln 
                85                  90                  95      


Leu Pro Asn Gln Cys Trp Glu Leu Arg Val Lys Gly Leu Asp Ser Leu 
            100                 105                 110         


Leu Ser Asn Thr Glu Trp Ala Ala Val Leu Leu His Leu Leu Lys His 
        115                 120                 125             


Arg Gly Tyr Leu Ser Gln Arg Lys Asn Glu Ala Gln Asn Ala Asp Lys 
    130                 135                 140                 


Glu Leu Gly Lys Leu Arg Glu Gly Met Asp Asn Asn Ser Lys Leu Leu 
145                 150                 155                 160 


Leu Glu Asn Asn Tyr Arg Thr Pro Ala Asp Ile Ala Val Lys Lys Phe 
                165                 170                 175     


Ala Val Glu Glu Gly His Met Arg Asn Gln Arg Gly Ala Tyr Thr His 
            180                 185                 190         


Thr Phe Asn Arg Leu Asp Ile Leu Ala Glu Met Gln Leu Leu Phe Lys 
        195                 200                 205             


Thr Gln Arg Glu Leu Gly Ser Thr Tyr Ala Asn Gly Glu Leu Glu Gln 
    210                 215                 220                 


Ala Phe Cys Glu Leu Leu Leu Trp Gln Lys Gln Ala Leu Asn Arg Ser 
225                 230                 235                 240 


Gln Met Leu Ser Leu Val Gly Lys Cys Thr Phe Glu Lys Glu Glu Lys 
                245                 250                 255     


Arg Ala Ala Lys Ala Ser Tyr Ser Ala Glu Arg Phe Val Ala Ile Gln 
            260                 265                 270         


Lys Leu Gln Asn Leu Arg Ile Leu Glu Asn Gly Glu Glu Arg Gly Leu 
        275                 280                 285             


Phe Asp Ser Glu Phe Ser Leu Leu Leu Glu Asn Ala Tyr Asn Leu Lys 
    290                 295                 300                 


Ser Gly Leu Thr Tyr Lys Gln Val Arg Lys Ile Leu Ser Leu Ser Glu 
305                 310                 315                 320 


Asn Ala Ile Phe Lys Gly Leu Pro Tyr Leu Ser Asp Asp Leu Glu Lys 
                325                 330                 335     


Pro Glu Lys Thr Gln Phe Leu Ala Phe Lys Phe Tyr His Gln Leu Ala 
            340                 345                 350         


Asp Ile Leu Lys Asn Asn Gly Phe Ser Asp Glu Trp Gln Lys Leu Ser 
        355                 360                 365             


Gln Glu Pro Thr Leu Leu Asp Lys Leu Gly Thr Glu Leu Ser Leu Cys 
    370                 375                 380                 


Lys Glu Glu Asn Glu Phe Ile Ala Gln Phe Asn Gly Glu Leu Pro Glu 
385                 390                 395                 400 


Ala Met Leu Ser Thr Leu Phe Asn His Thr Asn Phe Asp Lys Phe Ile 
                405                 410                 415     


His Ile Ser Leu Lys Ala Leu Asn Asn Ile Leu Pro Leu Met Glu Gln 
            420                 425                 430         


Gly Asn Asp Tyr Thr Lys Ala Trp Arg Lys Val Tyr Pro Glu Pro Thr 
        435                 440                 445             


Lys Lys Asp Glu Lys Thr Leu Pro Pro Ile Pro Ala Asp Glu Ile Arg 
    450                 455                 460                 


Asn Pro Val Val Leu Arg Ser Leu Ser Gln Ala Arg Lys Val Ile Asn 
465                 470                 475                 480 


Ala Val Ile Arg Leu Tyr Gly Ser Pro Ala Arg Ile His Ile Glu Thr 
                485                 490                 495     


Ala Arg Glu Leu Gly Lys Ser Tyr Asp Asp Arg Gln Lys Ile Lys Lys 
            500                 505                 510         


Gln Gln Asp Lys Asn Ser Asp Glu Arg Asp Gln Ala Val Lys Lys Phe 
        515                 520                 525             


Leu Glu Glu Cys Pro Asn Phe Ala Asn Lys Val Lys Gly Lys Asp Ile 
    530                 535                 540                 


Leu Lys Ile Arg Leu Tyr Ile Asn Gln Asp Gly Lys Cys Leu Tyr Ser 
545                 550                 555                 560 


Gly Lys Pro Leu Asp Pro His Arg Leu Leu Glu Ile Gly Tyr Val Glu 
                565                 570                 575     


Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Gln Asn 
            580                 585                 590         


Asn Lys Val Leu Val Leu Ala Asn Glu Asn Gln Asn Lys Gly Asn Gln 
        595                 600                 605             


Thr Pro Phe Glu Trp Leu Gly Lys Asp Glu His Gln Trp Ala Leu Phe 
    610                 615                 620                 


Val Ala Arg Val Asn Gly Cys Arg Phe Pro Tyr Ala Lys Lys Gln Arg 
625                 630                 635                 640 


Ile Leu Thr Lys Lys Leu Asp Glu Gln Gly Phe Leu Lys Arg Asn Leu 
                645                 650                 655     


Asn Asp Thr Arg Tyr Val Ser Ala Tyr Leu Met Lys His Ile Lys Glu 
            660                 665                 670         


Asn Leu His Leu Val Gly Lys Gly Ser Asp Lys Val Phe Ala Ser Asn 
        675                 680                 685             


Gly Gln Val Thr Asn Phe Leu Arg Arg Cys Trp Gly Leu Glu Lys Lys 
    690                 695                 700                 


Arg Glu Glu Gly Asp Arg His His Ala Leu Asp Ala Ile Val Val Ala 
705                 710                 715                 720 


Cys Ser Thr Ala Ser Met Arg Gln Lys Ile Thr Leu Phe Lys Lys Tyr 
                725                 730                 735     


Gln Arg Trp Asn Leu Lys Thr Gly Lys His Ile Asp Gln Glu Thr Gly 
            740                 745                 750         


Glu Ile Ile Pro Leu His Phe Pro Ala Pro Trp Asp Phe Phe Arg Gln 
        755                 760                 765             


Glu Val Met Ile Arg Ile Phe Ser Glu Met Pro Gln Glu Asp Leu Ile 
    770                 775                 780                 


Met Gln Leu Pro Asp Arg Pro Gln Ala Asn His Glu Phe Val Gln Pro 
785                 790                 795                 800 


Leu Phe Val Ser Arg Ala Pro Ser Arg Lys Met Ser Gly Gln Gly His 
                805                 810                 815     


Glu Ala Lys Leu Arg Ser Ala Arg Met Leu Glu Ala Thr Gly Lys Ser 
            820                 825                 830         


Ile Lys Lys Glu Phe Leu Thr Asp Leu Thr Ile Lys Asn Leu Glu His 
        835                 840                 845             


Met Val Asn Lys Glu Arg Glu Pro Glu Leu Tyr Gln Ala Leu Val Glu 
    850                 855                 860                 


His Phe Lys Lys Tyr Gly Asp Lys Pro Lys Glu Lys Phe Phe Lys Lys 
865                 870                 875                 880 


Gly Gly Val Glu Val Lys Ser Ile Arg Ile Ser Lys Thr Gln Asn Lys 
                885                 890                 895     


Ser Val Asn Leu Gly Asn Lys Thr Ile Ala Asp Asn Gly Asp Ile Val 
            900                 905                 910         


Arg Thr Asp Leu Phe Leu Lys Asn Lys Lys Tyr Tyr Phe Val Asn Ile 
        915                 920                 925             


Tyr Ala Trp Gln Val Ser Lys Gly Ile Leu Pro Lys Glu Thr Thr Thr 
    930                 935                 940                 


Gly Asn Ile Leu Asp Asn Ser Tyr Glu Phe Gln Phe Ser Leu Phe Lys 
945                 950                 955                 960 


Asn Asp Leu Leu Glu Ile Pro His Pro Lys Asn Glu Asn Asp Ser Ile 
                965                 970                 975     


Leu Ala Tyr Phe Ile Arg Pro Asp Asp Glu Arg Arg Trp Ile Leu Lys 
            980                 985                 990         


Phe His Asp Asn Ala Lys Ile Pro  Asp Ile Tyr Gly Lys  Lys Asp Glu 
        995                 1000                 1005             


Thr Ser  Ile Arg Leu Ser Ile  Gln Gly Gln Lys Phe  Ile Lys Lys 
    1010                 1015                 1020             


Tyr Gln  Val Asp Glu Leu Gly  Lys Asn Ile Arg Pro  Cys Arg Pro 
    1025                 1030                 1035             


Thr Lys  Arg Gln Gly Val Arg  
    1040                 1045 


<210>  512
<211>  1046
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  512

Met Lys Ile Tyr Ser Phe Asp Ile Gly Val Ala Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Ile Glu Asn Glu Val Leu Gln Asp Cys Gly Ile Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Asn Gly Ala Ser Leu Ala Leu Pro Arg Arg 
        35                  40                  45              


Glu Ala Arg Gly Val Arg Lys Arg Leu Ala Arg Arg Arg Gly Arg Leu 
    50                  55                  60                  


Asn Thr Ile Lys Gln Leu Leu Cys Lys Glu Phe Gly Leu Gln Leu Glu 
65                  70                  75                  80  


Asp Tyr Leu Ser Asn Asp Gly Glu Leu Pro Lys Ala Tyr Thr Ala Ser 
                85                  90                  95      


Lys Ala Asn Pro Leu Lys Ser Pro Tyr Glu Leu Arg Thr Leu Ala Leu 
            100                 105                 110         


Ser Gln Lys Leu Asp Ser Lys Asp Leu Val Arg Val Ile Leu His Ile 
        115                 120                 125             


Ala Lys His Arg Gly Tyr Gly Asn Lys His Ala Lys Asp Ser Lys Asp 
    130                 135                 140                 


Thr Glu Ser Gly Lys Val Lys Lys Ala Ile Glu Phe Asn Arg Lys Thr 
145                 150                 155                 160 


Leu Ala Glu Lys Gly Tyr Arg Ser Val Gly Glu Tyr Leu Tyr Arg Glu 
                165                 170                 175     


Phe Phe Gln Gln Ser Arg Ile Ser Gln Asn Asn Gly Ala Thr Glu Phe 
            180                 185                 190         


Val Asn Val Arg Asn Lys Thr Gly Asn Tyr Glu His Cys Val Ala Gln 
        195                 200                 205             


Asp Met Leu Lys Asp Glu Leu Glu Leu Ile Phe Arg Lys Gln Lys Glu 
    210                 215                 220                 


Leu Gly Leu His Leu Ser Asp Gly Phe Glu Lys Gln Leu Leu Asp Lys 
225                 230                 235                 240 


Ile Phe Glu Gln Arg Pro Leu Lys Ser Phe Ala Asp Lys Val Gly Lys 
                245                 250                 255     


Cys Gln Phe Ile Ala Gly Ala Lys Arg Ala Pro Lys Asp Ser Ile Ser 
            260                 265                 270         


Ala Ile Glu Phe Val Ala Leu Ser Arg Ile Ile Asn Thr Leu Ala Asn 
        275                 280                 285             


Leu Ser Lys Lys Ser Gly Glu Met Tyr Asp Lys Ala Met Val Leu Thr 
    290                 295                 300                 


Ile Leu Asp Ser Val Leu Glu Lys Gly Glu Met Ser Tyr Tyr Ala Leu 
305                 310                 315                 320 


Arg Glu Ala Ile Asn Leu Asp Glu Lys Ile Arg Phe Ala Asp Ser Arg 
                325                 330                 335     


Leu Asp Tyr Ser Lys Glu Ile Lys Glu Thr Glu Lys Val Lys Phe Val 
            340                 345                 350         


Glu Cys Lys Asn Leu Lys Ser Phe Lys Lys Val Leu Gly Asn Cys Tyr 
        355                 360                 365             


Lys Thr Leu Glu Arg Lys His Ile Asp Ser Ile Ala Glu Arg Ile Ala 
    370                 375                 380                 


Val Ile Lys Asp Val Glu Lys Leu His Lys Glu Leu Glu Ser Tyr Cys 
385                 390                 395                 400 


Ala Thr Glu Gln Leu Ser Leu Thr Ser Glu Gln Ile Gln Ala Leu Ser 
                405                 410                 415     


His Leu Asn Phe Ser Ser His Ile Ser Leu Ser Leu Lys Ala Leu Glu 
            420                 425                 430         


Gln Ile Leu Pro Phe Met Arg Gly Glu Arg Glu Ala Arg Ser Ser Asp 
        435                 440                 445             


Ala Asp Tyr Cys Ile Gly Ile Asp Glu Ser Gly Glu Ser Gln Cys Leu 
    450                 455                 460                 


Arg Tyr Asp Glu Ser Val Glu Lys Ala Gly Leu Lys Ala Leu Ser Lys 
465                 470                 475                 480 


Asp Thr Leu Lys Gly Gln Ile Leu Pro Pro Leu Asn Glu Phe Glu Pro 
                485                 490                 495     


Tyr Leu Ala Asn Pro Val Val Ala Arg Ala Leu Ala Glu Tyr Arg Lys 
            500                 505                 510         


Val Leu Asn Ala Leu Leu Lys Gln Tyr Gly Ser Pro His Lys Ile His 
        515                 520                 525             


Ile Glu Tyr Ala Arg Glu Ala Lys Leu Asn Thr Thr Glu Arg Gln Lys 
    530                 535                 540                 


Tyr Glu Lys Glu Gln Lys Asp Asn Tyr Ala Ala Asn Gln Ala Ala His 
545                 550                 555                 560 


Lys Lys Cys Gln Glu Leu Gly Leu Glu Pro Ser Ser Thr Asn Leu Leu 
                565                 570                 575     


Lys Leu Lys Leu Trp Gln Glu Gln Gly Gly Leu Cys Leu Tyr Ser Gly 
            580                 585                 590         


Ala Lys Ile Thr Ile Ser Tyr Leu Gln Asp Pro Thr Ala Leu Gln Val 
        595                 600                 605             


Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn 
    610                 615                 620                 


Lys Cys Leu Val Leu Thr Lys Ala Asn Gln Glu Lys Gly Asn Arg Thr 
625                 630                 635                 640 


Pro Phe Glu Ala Phe Gly Thr Asp Ser Ala Lys Trp Gly Val Ile Thr 
                645                 650                 655     


Thr Leu Ala Gln Lys Leu Pro His Lys Lys Arg Arg Arg Ile Leu Asn 
            660                 665                 670         


Thr Ala Phe Asn Asp Lys Glu Ala Gly Phe Lys Leu Arg Asn Leu Asn 
        675                 680                 685             


Asp Thr Ser Tyr Ile Ala Arg Leu Val Ala Asp Tyr Thr Glu Thr Tyr 
    690                 695                 700                 


Leu Glu Phe Leu Ser Leu Val Glu Ser Glu Asn Thr Thr Leu Gly Lys 
705                 710                 715                 720 


Gly Gln Lys Gly Ser Lys Lys His Ile Ala Ile Val Asn Gly Ala Leu 
                725                 730                 735     


Thr Ser Thr Met Arg Tyr Tyr Leu Gly Phe Ala Ser Lys Asp Arg Asp 
            740                 745                 750         


Asn His Leu His His Ala Leu Asp Ala Val Ile Ile Gly Phe Met Asn 
        755                 760                 765             


Asp Ser Val Ile Lys Ala Phe Ser Asp Phe Lys Lys Thr Gln Glu Thr 
    770                 775                 780                 


Ser Lys Ala Ala Tyr Tyr Ala His Thr Leu Ser Lys Gln Glu Tyr Lys 
785                 790                 795                 800 


Lys Gln Arg Ala Phe Ile Gln Leu Pro Ser Gly Glu Asn Phe Arg Ala 
                805                 810                 815     


Asn Val Leu Glu Lys Ala Glu Ser Val Phe Val Ser Lys Pro Pro Arg 
            820                 825                 830         


Lys Arg Val Arg Gly Ala Leu His Glu Glu Ser Phe Tyr Ser Gln Asn 
        835                 840                 845             


Asp Thr Lys Leu Leu Lys Ser Tyr Gly Gly Ala Glu Gly Ile Glu Lys 
    850                 855                 860                 


Ala Leu Gln Leu Gly Lys Ile Arg Gln Ile Gly Thr Lys Ile Val Ser 
865                 870                 875                 880 


Asn Gly Ala Met Val Arg Val Asp Ile Phe Lys His Arg Lys Ser Gly 
                885                 890                 895     


Lys Phe Tyr Gly Val Pro Val Tyr Thr Met Asp Phe Ala Leu Gly Ile 
            900                 905                 910         


Leu Pro Asn Lys Ala Val Val Gln Gly Lys Asp Lys Asn Asp Val Ile 
        915                 920                 925             


Lys Asp Trp Leu Glu Met Asp Ser Ser Tyr Glu Phe Ile Phe Ser Leu 
    930                 935                 940                 


Tyr Lys Asp Asp Leu Leu Leu Val Gln Lys Lys Glu Met Ala Glu Pro 
945                 950                 955                 960 


Glu Leu Cys Tyr Phe Val Ser Phe Asp Thr Ser Gly Ala Ser Ile Lys 
                965                 970                 975     


Val Ala Lys His Asp Asn Asn Phe Ser Thr Leu Thr Glu Asn Gln Lys 
            980                 985                 990         


Leu Leu Phe Ser Asn Ala Thr Lys  Glu Glu Val Val Gly  Lys Ser Ile 
        995                 1000                 1005             


Gly Ile  Gln Asn Leu Lys Val  Phe Glu Lys Trp Gln  Val Ser Val 
    1010                 1015                 1020             


Leu Gly  Glu Val Lys Arg Thr  Gln Ser Tyr Pro Arg  Glu Asn Ile 
    1025                 1030                 1035             


Ser Leu  Ala Ser Lys Ala Lys  Lys 
    1040                 1045     


<210>  513
<211>  1047
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  513

Met Lys Leu Leu Arg Tyr Ile Leu Gly Leu Asp Leu Gly Ile Ala Ser 
1               5                   10                  15      


Val Gly Trp Ala Ile Val Glu Ile Asp Glu Asn Asp Phe Pro Ile Arg 
            20                  25                  30          


Leu Ile Asp Ile Gly Val Arg Thr Phe Asp Lys Ala Glu Val Ser Lys 
        35                  40                  45              


Thr Gly Glu Ser Leu Ala Leu Ala Arg Arg Leu Ala Arg Ser Thr Arg 
    50                  55                  60                  


Arg Leu Ile Arg Arg Arg Ala Phe Arg Leu Leu Lys Gly Lys Arg Leu 
65                  70                  75                  80  


Leu Lys Gln His Asn Val Ile Asn Lys Glu Ala Leu Thr Thr Leu Pro 
                85                  90                  95      


Thr Asp Cys Trp Ala Leu Arg Val Lys Gly Leu Asp Glu Arg Leu Ser 
            100                 105                 110         


Pro Thr Glu Trp Ser Ala Val Leu Leu His Leu Leu Lys His Arg Gly 
        115                 120                 125             


Tyr Leu Ser Gln Arg Lys Asn Glu Ala Lys Ser Ala Asp Lys Glu Met 
    130                 135                 140                 


Gly Ala Leu Leu Ser Gly Val Ala Ser Asn His Gln Leu Leu Val Glu 
145                 150                 155                 160 


Asn Gln Phe Arg Thr Pro Ala Glu Ile Ala Val Lys Lys Phe Ala Leu 
                165                 170                 175     


Glu Glu Gly His Ile Arg Asn Gln Leu Gly Ala Tyr Thr His Thr Phe 
            180                 185                 190         


Asn Arg Leu Asp Leu Leu Ala Glu Met Lys Leu Leu Phe Gln Thr Gln 
        195                 200                 205             


Arg Glu Leu Gly Asn Pro His Thr Ser Gly Gln Leu Glu Gln Glu Phe 
    210                 215                 220                 


Cys Glu Leu Leu Met Trp Gln Lys Pro Ala Leu Ser Gly Asp Ala Ile 
225                 230                 235                 240 


Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Thr Asn Glu Tyr Lys Ala 
                245                 250                 255     


Ala Lys His Ser Tyr Thr Ala Glu Arg Phe Val Trp Leu Thr Lys Leu 
            260                 265                 270         


Asn Asn Leu Arg Ile Leu Glu His Gly Ser Glu Arg Val Leu Asn Thr 
        275                 280                 285             


Asp Glu Arg Ser Leu Leu Leu Asn Gln Pro Tyr Glu Lys Ala Lys Leu 
    290                 295                 300                 


Thr Tyr Thr Gln Val Arg Lys Leu Leu Gly Leu Ser Glu Glu Ala Arg 
305                 310                 315                 320 


Phe Asn Gly Val Arg Tyr Gly Lys Glu Lys Ala Glu Asp Ala Thr Leu 
                325                 330                 335     


Ile Glu Leu Lys Ala Tyr His Ala Ile Arg Lys Ala Val Glu Lys Ala 
            340                 345                 350         


Asp Lys Ser Ala Trp Glu Thr Leu Lys Asn Gln Thr Ala Leu Leu Asp 
        355                 360                 365             


Glu Ile Gly Thr Thr Phe Ser Ile Tyr Lys Thr Asp Asp Asp Ile Thr 
    370                 375                 380                 


Glu Gln Leu Asn Gly Lys Leu Ser Asp Thr Val Leu Asn Ala Leu Leu 
385                 390                 395                 400 


Glu Asn Leu Asn Phe Asp Lys Phe Ile Gln Leu Ser Leu Lys Ser Leu 
                405                 410                 415     


Tyr Asn Ile Val Pro Leu Met Glu Gln Gly Leu Arg Tyr Asp Glu Ala 
            420                 425                 430         


Cys Gln Gln Ile Tyr Gly Asp His Phe Gly Lys Lys Ser Gly Glu Ile 
        435                 440                 445             


Asn Arg Leu Leu Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val 
    450                 455                 460                 


Val Leu Arg Ser Leu Thr Gln Ala Arg Lys Val Ile Asn Ala Val Ile 
465                 470                 475                 480 


Arg Leu Tyr Gly Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu 
                485                 490                 495     


Leu Gly Lys Ser Tyr Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu 
            500                 505                 510         


Gln Asn Arg Ala Glu Arg Glu Lys Ala Thr Ala Gln Phe Lys Thr Leu 
        515                 520                 525             


Phe Pro Thr Phe Ile Gly Glu Pro Lys Gly Lys Asp Ile Leu Met Met 
    530                 535                 540                 


Arg Leu Tyr Glu Gln Gln Asn Gly Gln Cys Leu Tyr Ser Gly Lys Pro 
545                 550                 555                 560 


Leu Asn Leu Asn Arg Leu Leu Glu Lys Gly Tyr Val Glu Ile Asp His 
                565                 570                 575     


Ser Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Gln Asn Asn Lys Val 
            580                 585                 590         


Leu Val Leu Ala Gly Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Phe 
        595                 600                 605             


Glu Trp Leu Gly Lys Asp Glu His Gln Trp Ala Leu Phe Val Ala Arg 
    610                 615                 620                 


Val Asn Gly Cys Arg Phe Pro Tyr Ala Lys Lys Gln Arg Ile Leu Thr 
625                 630                 635                 640 


Gln Lys Leu Asp Glu Gln Gly Phe Ile Glu Arg Asn Leu Asn Asp Thr 
                645                 650                 655     


Arg Tyr Ile Ala Arg Phe Leu Val Asn Phe Val Arg Glu Asn Leu His 
            660                 665                 670         


Leu Thr Gly Lys Gly Lys Asn Arg Val Phe Ala Ser Asn Gly Gln Ile 
        675                 680                 685             


Thr Ala Phe Leu Arg Gly Arg Trp Gly Leu Ser Lys Val Arg Glu Glu 
    690                 695                 700                 


Ser Asp Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr 
705                 710                 715                 720 


Val Ala Met Gln Gln Lys Ile Thr Arg Phe Val Lys Tyr Gln Glu Gly 
                725                 730                 735     


Asn Leu Gln Thr Ala Glu Arg Ile Asp Arg Glu Thr Gly Glu Ile Ile 
            740                 745                 750         


Pro Leu His Phe Pro Asp Pro Trp His Phe Phe Arg Gln Glu Val Met 
        755                 760                 765             


Ile Arg Ile Phe Ser Glu Asn Pro Gln Glu Asp Leu Ile Ile Gln Leu 
    770                 775                 780                 


Pro Asp Arg Pro Glu Ala Asn His Glu Phe Val Gln Pro Leu Phe Val 
785                 790                 795                 800 


Ser Arg Met Pro Thr Arg Lys Met Ser Gly Gln Gly His Met Glu Thr 
                805                 810                 815     


Ile Lys Ser Ala Lys Arg Leu Asp Glu Gly Ile Ser Val Ser Lys Val 
            820                 825                 830         


Pro Leu Thr Gln Leu Lys Leu Lys Asp Leu Asp Asn Met Val Asn Arg 
        835                 840                 845             


Glu Arg Glu Lys Ala Leu Tyr Glu Ala Leu Lys Ala Arg Leu Asn Ala 
    850                 855                 860                 


Phe Asn Asp Asp Pro Thr Lys Ala Phe Ala Glu Pro Phe Tyr Lys Lys 
865                 870                 875                 880 


Gly Gly Gln Gln Val Lys Ala Val Arg Val Glu Gln Val Gln Lys Ser 
                885                 890                 895     


Gly Val Val Val Arg Asn Gly Asn Gly Val Ala Asp Asn Ala Ser Met 
            900                 905                 910         


Val Arg Val Asp Val Phe Ile Lys Gly Gly Lys Tyr Phe Leu Val Pro 
        915                 920                 925             


Ile Tyr Thr Trp Gln Val Ala Lys Gly Ile Leu Pro Asp Arg Ala Val 
    930                 935                 940                 


Val Ala Ala Lys Asp Glu Ser Glu Trp Glu Leu Met Asp Lys Thr Ala 
945                 950                 955                 960 


Gln Phe Leu Phe Ser Met Phe Pro Asn Asp Leu Ile Gln Val Lys Thr 
                965                 970                 975     


Lys Lys Glu Thr Phe Phe Gly Tyr Tyr Asn Gly Leu Asp Arg Ala Thr 
            980                 985                 990         


Gly Ala Ile Asn Leu Lys Glu Pro  Asp Ser Asn Ile Asn  Lys Gly Lys 
        995                 1000                 1005             


Ser Gly  Leu His Arg Ser Ile  Gly Val Lys Thr Ala  Leu Ser Val 
    1010                 1015                 1020             


Glu Lys  Tyr Gln Val Asp Glu  Leu Gly Lys Asn Ile  Arg Pro Cys 
    1025                 1030                 1035             


Arg Pro  Ser Lys Arg Gln Ala  Val Arg 
    1040                 1045         


<210>  514
<211>  1048
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  514

Met Lys Arg Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly Trp Cys 
1               5                   10                  15      


Leu Ile Glu Asp Asp Gly Asp Gly Lys Gly Arg Ile Ile Asp Ile Gly 
            20                  25                  30          


Ala Arg Ile Phe Ser Asp Gly Arg Asp Pro Lys Ser Gly Ala Ser Leu 
        35                  40                  45              


Ala Val Asp Arg Arg Ala Ala Arg Ala Met Arg Arg Arg Arg Asp Arg 
    50                  55                  60                  


Tyr Leu Gly Arg Arg Thr Ala Phe Leu Lys Ala Leu Lys Arg Phe Gly 
65                  70                  75                  80  


Leu Met Pro Ala Asp Glu Ser Ala Ala Lys Leu Leu Ala Asp Arg Asp 
                85                  90                  95      


Pro Tyr Glu Leu Arg Ala Arg Ala Leu Asp Glu Arg Leu Glu Pro His 
            100                 105                 110         


Glu Ile Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ala Glu Arg Lys Thr Lys Asp Ala Glu Asp Gly Lys 
    130                 135                 140                 


Ile Ala Asn Gly Ala Lys Ala Leu Asp Gln Ala Met Ala Glu Ser Gly 
145                 150                 155                 160 


Ala Glu Thr Leu Gly Gln Phe Leu Ser Ser Arg Asp Thr Lys Arg Val 
                165                 170                 175     


Arg Met Gly Ser Asp Ala Gln Gly Tyr Asp Phe Tyr Pro Gln Arg Arg 
            180                 185                 190         


His Val Glu Tyr Glu Phe Asp Thr Ile Trp Ser Arg Gln Gln Gln His 
        195                 200                 205             


His Pro Ala Leu Leu Thr Glu Glu Ala Arg Lys Lys Leu His Asp Ile 
    210                 215                 220                 


Leu Phe Phe Gln Arg Pro Leu Lys Glu Gln Glu Val Gly Val Cys Thr 
225                 230                 235                 240 


Phe Val His Asn Glu Cys Arg Leu Pro Lys Ala His Pro Leu Phe Gln 
                245                 250                 255     


Glu Arg Arg Leu Tyr Glu Glu Val Asn Gln Leu Glu Ile Thr Thr Pro 
            260                 265                 270         


Gly Ala Pro Asn Arg Lys Leu Thr Met Asp Glu Arg Asp Ala Leu Ile 
        275                 280                 285             


Leu Glu Leu Arg Ser Arg Lys Glu Arg Ser Phe Asp Ser Leu Ala Lys 
    290                 295                 300                 


Arg Leu Lys Leu Ala Pro Gly Gln Ser Phe Asn Lys Ala Ser Glu Ser 
305                 310                 315                 320 


Arg Thr Arg Leu Arg Gly Asp Glu Val Tyr Ala Val Met Ala His Lys 
                325                 330                 335     


Lys Arg Phe Gly Asp Arg Trp Pro His Leu Asp Arg Asp Arg Gln Trp 
            340                 345                 350         


Ala Ile Ile Lys Arg Ile Ile Glu Glu Glu Asp Pro Asp Lys Leu His 
        355                 360                 365             


Ala Phe Leu Thr Gly Glu Trp Gly Leu Thr Asp Glu Gln Ala Leu Ala 
    370                 375                 380                 


Thr Ala Arg Ala Pro Leu Pro Glu Leu Tyr Gly Arg Leu Gly Pro Thr 
385                 390                 395                 400 


Ala Thr Ala Arg Ile Leu Glu Glu Leu Lys Lys Asp Val Ile Thr Tyr 
                405                 410                 415     


Asp Glu Ala Val Arg Arg Ala Gly Trp His His Ser Asp Asp Arg Thr 
            420                 425                 430         


Gly Glu Ile Leu Pro Gln Leu Pro Tyr Tyr Gly Glu Leu Leu Thr Arg 
        435                 440                 445             


Asp Ile Pro Pro Gly Thr Gln Asp Pro Asn Asp Pro Pro Glu Gln Arg 
    450                 455                 460                 


Trp Gly Lys Ile Thr Asn Pro Thr Val His Ile Gly Leu Arg Gln Leu 
465                 470                 475                 480 


Glu Lys Leu Val Asn Ala Ile Ile Lys Val His Gly Arg Pro Asp Gln 
                485                 490                 495     


Ile Val Val Glu Leu Ala Arg Glu Leu Lys Leu Asn Glu Lys Asp Lys 
            500                 505                 510         


Glu Glu His Asn Arg Arg Ile Arg Arg Asp Thr Ala Ala Ala Gln Leu 
        515                 520                 525             


Arg Ser Ala Lys Leu Gln Ser Glu Gly Ile Pro Asp Thr Gly Ala Asn 
    530                 535                 540                 


Arg Met Leu Leu Arg Ile Trp Glu Glu Leu Asn Pro Gly Glu Val Leu 
545                 550                 555                 560 


Asp Arg Arg Cys Pro Tyr Cys Gly Glu Pro Val Gly Met Arg Met Leu 
                565                 570                 575     


Phe Ala Gly Glu Ala Asp Ile Asp His Ile Ile Pro Tyr Ser Arg Ser 
            580                 585                 590         


Leu Asp Asp Ser Ala Gly Asn Lys Val Val Ala His Arg Ser Cys Asn 
        595                 600                 605             


Arg Ala Lys Gly Asn Arg Thr Pro Tyr Glu Lys Trp Val His Asp Pro 
    610                 615                 620                 


Asp Arg Trp Asp Val Ile Ser Thr Gln Val Ala Arg Leu His Lys Ser 
625                 630                 635                 640 


Lys Gln Trp Arg Phe Gly Pro Asp Ala Met Ala Arg Val Glu Lys Asp 
                645                 650                 655     


Gly Gly Phe Ile Ala Arg Gln Leu Thr Asp Thr Gln Tyr Leu Ser Arg 
            660                 665                 670         


Leu Ala Gly Arg Tyr Leu Ser Ser Leu Tyr Pro Thr Lys Asp Asp Gly 
        675                 680                 685             


Ser Val Tyr Val Ile Pro Gly Arg Met Thr Ala Met Leu Arg Arg Arg 
    690                 695                 700                 


Trp Gly Leu Asn Ser Leu Leu Pro Asp His Asn Phe Val Asp Asn Glu 
705                 710                 715                 720 


His Ser Asn Ala Pro Lys Asn Arg Leu Asp His Arg His His Ala Ile 
                725                 730                 735     


Asp Ala Ala Val Val Ala Val Thr Thr Arg Ala Leu Met Gln Glu Ile 
            740                 745                 750         


Ala Arg Thr Ala Gly Arg Ala Glu Asp Lys Asp Leu Asp Arg Leu Phe 
        755                 760                 765             


Glu Asp Leu Pro Gln Pro Trp Glu Gly Phe Arg Glu Glu Leu Gly Glu 
    770                 775                 780                 


Lys Leu Val Arg Val Thr Val Ser His Lys Pro Asp His Gly Arg Lys 
785                 790                 795                 800 


Gly Leu Pro Pro Lys Asn Arg Asp Val Thr Ala Gly Arg Leu His Asn 
                805                 810                 815     


Asp Thr Ala Tyr Gly Leu Thr Gly Asp Val Ala Ala Asp Gly Lys Thr 
            820                 825                 830         


Pro Ile Val Val His Arg Val Pro Leu Met Ser Leu Lys Pro Ala Asp 
        835                 840                 845             


Ile Ser Asp Pro Asp Arg Ile Pro Asp Pro Ala Leu Arg Ser Ala Leu 
    850                 855                 860                 


His Arg Ala Thr Asp Gly Leu Ser Gly Lys Ala Tyr Asp Ala Ala Leu 
865                 870                 875                 880 


Ala Gly Phe Ala Lys Thr His Pro Gln Phe Ala Gly Ile Arg Arg Val 
                885                 890                 895     


Arg Val Arg Glu Pro Leu Thr Val Ile Pro Ile Ala Asp Arg Asp Gly 
            900                 905                 910         


Arg Pro Phe Lys Ala Tyr Lys Gly Asp Ala Asn Ser Arg Val Asp Ile 
        915                 920                 925             


Trp Arg Met Pro Asp Gly Lys Trp Leu Ser Asp Val Val Thr Met Tyr 
    930                 935                 940                 


Glu Ala His Arg Arg Asp His Ser Asp Arg Arg Pro His Pro Ala Ala 
945                 950                 955                 960 


Lys Lys Leu Met Ser Leu Arg Gln Asn Asp Met Ile Ala Val Glu Arg 
                965                 970                 975     


Asp Gly Ala Pro Arg Gln Ile Met Val Val Lys Gln Ile Trp Pro Thr 
            980                 985                 990         


Gln Ile Asn Leu Val Glu His Lys  Glu Thr Gly Asn Leu  Lys Asp Arg 
        995                 1000                 1005             


His Ser  Asn Glu Thr Asp Pro  Phe Arg Phe Phe Ala  Pro Thr Ala 
    1010                 1015                 1020             


Gly Gly  Leu Lys Lys Met Lys  Ala Arg Gln Val Arg  Ile Asp Pro 
    1025                 1030                 1035             


Leu Gly  Arg Ile Phe Asp Pro  Gly Pro Arg 
    1040                 1045             


<210>  515
<211>  1048
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  515

Met Ala Asn Ile Ala Glu Asn Leu Val Phe Gly Ile Asp Leu Gly Ile 
1               5                   10                  15      


Gly Ser Cys Gly Trp Ala Val Leu Asn Gln Lys Ala Lys Asp Glu Asn 
            20                  25                  30          


Ile Val Ala Leu Gly Ser Trp Cys Phe Asp Val Pro Glu Thr Asp Lys 
        35                  40                  45              


Glu Arg Ile Pro Thr Asn Gln Ile Arg Arg Ala Asn Arg Leu Leu Arg 
    50                  55                  60                  


Arg Val Ile Arg Arg Arg Arg Asn Arg Met Ala Glu Ile Arg Arg Leu 
65                  70                  75                  80  


Phe Ala Ser Lys Gly Met Leu Asp Thr Ala Asp Pro Asp Ala Leu Ala 
                85                  90                  95      


Lys Ile Ser Ala Asp Pro Trp Glu Met Arg Ala Lys Gly Leu Asp Tyr 
            100                 105                 110         


Gln Leu Thr Pro Gln Glu Phe Ala Val Ala Leu Gly His Ile Ala Lys 
        115                 120                 125             


Arg Arg Gly Phe Lys Ser Ala Ala Lys Arg Val Ser Thr Asn Ala Pro 
    130                 135                 140                 


Ser Glu Asp Ser Lys Met Leu Ala Ala Leu Glu Lys Thr Lys Glu Gln 
145                 150                 155                 160 


Ser Ala Gln Tyr Arg Thr Ile Gly Glu Met Phe Ala Arg Asp Pro Ala 
                165                 170                 175     


Tyr Ala Asp Arg Arg Arg Asn Arg Asp Gly Cys Phe Asp Arg Thr Met 
            180                 185                 190         


Ser Arg Glu Asp Leu Phe Lys Glu Thr Gln Ile Leu Phe Glu Ser Gln 
        195                 200                 205             


Arg Arg Leu Gly Asn Phe Ser Ala Thr Ser Glu Thr Glu Gln Ala Phe 
    210                 215                 220                 


Ile Asp Ile Ala Phe Arg Gln Lys Pro Met Gln Asp Ser Glu Arg Leu 
225                 230                 235                 240 


Val Glu Thr Cys Leu Phe Glu Pro Asp Glu Lys Arg Ala Ser Arg Phe 
                245                 250                 255     


Ala Pro Ser Phe Glu Arg Phe Arg Leu Leu Cys Arg Leu Val Asn Leu 
            260                 265                 270         


Arg Val Thr Asp Gly Leu Glu Asp Arg Pro Leu Thr Pro Glu Glu Leu 
        275                 280                 285             


Arg Leu Ala Cys Glu Asp Ala Gly Lys Thr Arg Lys Leu Ser Val Lys 
    290                 295                 300                 


Lys Val Arg Ser Leu Ile Gly Leu Arg Asp Asp Gln Arg Phe Thr Thr 
305                 310                 315                 320 


Ile Pro Pro Asp Gln Glu Gln His Asp Ile Ala Ala Arg Thr Gly Asp 
                325                 330                 335     


Ala Leu Thr Gly Thr Ala Thr Phe Arg Ala Ile Leu Gly Glu Ser Phe 
            340                 345                 350         


Trp Arg Gln Leu Leu Pro His Pro Glu Gln Leu Asp Arg Ala Ala Trp 
        355                 360                 365             


Ile Ile Ser Phe His Glu Leu Thr Asp Thr Ile Ile Ser Lys Leu Ala 
    370                 375                 380                 


Thr Ile Gly Leu Pro Ser Glu Val Met Glu Leu Ile Arg Lys Ala Leu 
385                 390                 395                 400 


Glu Asp Gly Lys Phe Ser Lys Phe Lys Gly Ala Ala Ser Leu Ser Asp 
                405                 410                 415     


Lys Ala Ala Arg Lys Leu Ile Pro Phe Leu Gln Glu Gly Leu Thr Tyr 
            420                 425                 430         


Asp Lys Ala Cys Gln Lys Ala Gly Tyr Asn His Ala Ala Ser Lys Phe 
        435                 440                 445             


Thr His Tyr Asp Ser Ile Asp Thr Lys Ala Lys Phe Asn Ser Leu Met 
    450                 455                 460                 


Asp Asp Val Arg Asp Ser Ile Thr Asn Pro Val Ala Arg Lys Ala Ile 
465                 470                 475                 480 


Ser Glu Gly Met Lys Gln Val Trp Ala Met Arg Asn Gln Trp Gly Leu 
                485                 490                 495     


Pro Gly Ala Ile Cys Ile Glu Leu Ala Arg Asp Val Gly Asn Ser Ala 
            500                 505                 510         


Glu Lys Arg Arg Glu Ile Glu Ser Gly Ile Lys Lys Asn Thr Ala Gln 
        515                 520                 525             


Arg Glu Arg Glu Arg Gln Glu Ala Lys Glu His Leu Asn Gln Ser Asp 
    530                 535                 540                 


Ile Ser Ser Glu Thr Leu Leu Arg Tyr Arg Leu Trp Lys Glu Gln Gly 
545                 550                 555                 560 


Gly Arg Cys Cys Tyr Thr Asp Gln Pro Ile Thr Pro Ser Gln Leu Ile 
                565                 570                 575     


Ala Ser Asp Asn Ser Val Gln Val Asp His Ile Leu Pro Trp Ser Arg 
            580                 585                 590         


Phe Gly Asp Asp Ser Tyr Leu Asn Lys Ala Leu Cys Thr Ala Lys Ala 
        595                 600                 605             


Asn Gln Glu Lys Lys Asn Arg Thr Pro Trp Glu Trp Ile His Asn Gln 
    610                 615                 620                 


Lys Gly Val Glu Ala Trp Asn His Phe Thr Ala Ala Val Glu Ser Arg 
625                 630                 635                 640 


Lys Glu Thr Arg Gly Leu Lys Lys Arg Asn Phe Leu Leu Lys Ser Ser 
                645                 650                 655     


Lys Glu Thr Glu Glu Arg Phe Arg Ser Arg Asn Leu Asn Asp Thr Arg 
            660                 665                 670         


Tyr Ala Ala Arg Val Met Ala Glu Ala Val Arg Leu Leu Tyr Pro Ser 
        675                 680                 685             


Gly Asn Arg Ala Glu Lys Gly Gly Val Lys Arg Val Phe Thr Arg Pro 
    690                 695                 700                 


Gly Ala Leu Thr Ala Ala Leu Arg His Ala Trp Gly Val Glu Ser Leu 
705                 710                 715                 720 


Lys Lys Ile Asn Gly Lys Arg Leu Asn Asp Asp Arg His His Ala Leu 
                725                 730                 735     


Asp Ala Ala Val Val Ala Ala Val Ser Glu Lys Glu Ile Gln Lys Leu 
            740                 745                 750         


Thr Lys Ser Phe Gln Ile Cys Glu Gln Lys Gly Leu Ala Arg Pro Met 
        755                 760                 765             


Arg Asp Val Pro Met Pro Trp Glu Gly Phe Arg His Gln Leu Glu Thr 
    770                 775                 780                 


Ala Tyr Ala Asp Ile Arg Val Ala Arg Pro Glu Arg Arg Arg Ala Arg 
785                 790                 795                 800 


Gly Lys Gly His Asp Ala Thr Ile Arg Gln Leu Arg Glu Glu Lys Asp 
                805                 810                 815     


Gly Pro Val Val Tyr Glu Arg Arg Ala Ile Ser Asp Leu Lys Leu Thr 
            820                 825                 830         


Asp Leu Glu Arg Ile Lys Asp Pro Glu Arg Asn Ser Ala Ile Ile Ser 
        835                 840                 845             


Ser Leu Arg Thr Trp Ile Glu Lys Gly Lys Pro Ala Asp Tyr Pro Pro 
    850                 855                 860                 


Gln Ser Pro Lys Gly Asp Glu Ile Arg Lys Ile Arg Val Met Ser Arg 
865                 870                 875                 880 


Asn Lys Pro Ala Val Ser Val Arg Gly Gly Thr Ala Asp Arg Gly Glu 
                885                 890                 895     


Met Thr Arg Val Asp Val Phe Thr Lys Met Gly Lys Lys Gly Lys Pro 
            900                 905                 910         


Glu Trp Phe Leu Val Pro Ile Tyr Arg His Gln Val Ile Asp Arg Ile 
        915                 920                 925             


Gln Trp Pro Gln Pro Pro Asn Gln Ala Ala Ala Asn Gly Lys Ser Glu 
    930                 935                 940                 


Lys Asn Trp Pro Thr Met Gly Leu Glu His Glu Phe Arg Phe Ser Leu 
945                 950                 955                 960 


Tyr Pro Arg Ser Tyr Val Gln Ile Val Lys Arg Asp Gly Glu Ile Ile 
                965                 970                 975     


Glu Gly Tyr Phe Ala Gly Leu Asp Arg Thr Val Ala Ala Phe Ser Leu 
            980                 985                 990         


Leu Ser Ser Ser Asp Pro Thr Ser  Ile Gln Arg Gly Ile  Gly Ala Lys 
        995                 1000                 1005             


Thr Leu  Glu Ser Ile Arg Lys  Tyr Asn Val Asn Arg  Phe Gly Asp 
    1010                 1015                 1020             


Ile Ser  Met Val Glu Gln Glu  Thr Arg Thr Trp His  Gly Asp Val 
    1025                 1030                 1035             


Cys Ile  Ser Pro Asp Gln Pro  Asp Ser Ala 
    1040                 1045             


<210>  516
<211>  1048
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  516

Met Val Glu Lys Ile Leu Gly Ile Asp Leu Gly Ile Ser Ser Leu Gly 
1               5                   10                  15      


Trp Ala Val Val Glu Tyr Asp Lys Asp Asn Asp Glu Asn Asn Lys Ile 
            20                  25                  30          


Ile Asp Cys Gly Val Arg Leu Phe Thr Ala Ala Glu Thr Pro Lys Glu 
        35                  40                  45              


Lys Glu Ser Pro Asn Lys Ala Arg Arg Asp Ala Arg Gly Leu Arg Arg 
    50                  55                  60                  


Val Ile Lys Arg Arg Arg Val Arg Met Asn Thr Ile Lys Asn Leu Leu 
65                  70                  75                  80  


Ile Thr Tyr Lys Leu Ile Asp Lys Thr Leu Leu Asp Glu Glu Met Gly 
                85                  90                  95      


Met Phe His Ser Gln Ser Asn Arg Val Asp Val Trp Lys Leu Arg His 
            100                 105                 110         


Asp Ala Leu Tyr His Leu Leu Ser Gly Asp Glu Leu Ala Arg Val Leu 
        115                 120                 125             


Ile His Ile Ala Lys His Arg Gly Tyr Lys Phe Leu Gly Asp Asp Glu 
    130                 135                 140                 


Ser Asp Glu Glu Ser Gly Lys Val Lys Lys Ala Gly Ala Glu Leu Lys 
145                 150                 155                 160 


Lys Lys Phe Leu Glu Ala Gly Cys Gln Ser Val Gly Glu Trp Leu Trp 
                165                 170                 175     


Lys Glu Arg Gly Leu Gln Gly Lys Lys Arg Asn Lys Ser Gly Asp Tyr 
            180                 185                 190         


Glu Ile Ser Ile Pro Arg Asp Phe Leu Val Glu Glu Ile Gln Arg Ile 
        195                 200                 205             


Phe Glu Thr Gln Gln Lys Phe Gly Ser Thr Phe Ala Thr Ser Glu Leu 
    210                 215                 220                 


Gln Lys Ala Tyr Thr Asp Ile Ala Phe Tyr Val Lys Pro Met Gln Ser 
225                 230                 235                 240 


Ile Glu Asp Met Val Gly Tyr Cys Thr Phe Tyr Pro Lys Ile Lys Ser 
                245                 250                 255     


Lys Asn Gln Asp Gly Glu Lys Arg Ala Pro Lys Ala Ser Pro Ser Ala 
            260                 265                 270         


Glu Gln Phe Val Ile Leu Ser Lys Ile Phe Ser Thr Ile Val Ile Asp 
        275                 280                 285             


Glu Asn Lys Gln Glu Lys Lys Leu Ile Glu Leu Lys Ser Ile Glu Gln 
    290                 295                 300                 


Leu Ile Gln Ile Ala Arg Ser Lys Glu Thr Leu Lys Tyr Lys Gln Leu 
305                 310                 315                 320 


Arg Lys Glu Leu Asn Leu Ala Lys Asp Ile Ser Phe Lys Ser Ile Ser 
                325                 330                 335     


Asp Glu Glu Lys Thr Trp Ile Asn Leu Val Gly Asn Ala Lys Phe Lys 
            340                 345                 350         


Lys Ile Leu Gly Leu Asn Tyr Glu Thr Phe Leu Lys Asn Thr Glu Ile 
        355                 360                 365             


Ser Asp Glu Ile Ala Lys Ile Leu Thr Tyr Asp Lys Thr Phe Glu Gln 
    370                 375                 380                 


Lys Glu Thr Lys Leu Lys Asn Leu Leu Val Asn Ile Asp Trp Ile Asp 
385                 390                 395                 400 


Asn Asn His Ile Ala Glu Leu Ala Lys Leu Ser Phe Ser Gln Phe Asn 
                405                 410                 415     


Gln Leu Ser Leu Lys Ala Ile Lys Ile Ile Ser Lys Ile Met Ile Glu 
            420                 425                 430         


Gly Tyr Ala Arg Tyr Asp Glu Ala Val Gln Tyr Ala Phe Glu Asn Asn 
        435                 440                 445             


Leu Leu Pro Lys Pro Ser His Glu Lys Ser Ile Leu Leu Pro Pro Leu 
    450                 455                 460                 


Lys Glu Thr Asn Ile Ala Ile Leu Asn Pro Thr Val Ile Arg Ala Phe 
465                 470                 475                 480 


Ala Gln Phe Arg Gln Val Ala Asn Ala Leu Val Ser Lys Tyr Gly Ser 
                485                 490                 495     


Phe Asp Lys Val His Phe Glu Leu Ala Arg Glu Val Asn Thr Lys Glu 
            500                 505                 510         


Asp Arg Lys Arg Trp Glu Lys Asp Arg Asp Lys Asn Glu Lys Met His 
        515                 520                 525             


Arg Gln Ile Thr Glu Lys Leu Val Glu Glu Gly Val Lys Pro Ser Tyr 
    530                 535                 540                 


Lys Asn Ile Leu Lys Ser Lys Leu Arg Ser Glu Gln Lys Asp Thr Cys 
545                 550                 555                 560 


Pro Tyr Cys Gln Lys Asn Leu His Tyr Pro Met Ile Phe Glu Asp Gly 
                565                 570                 575     


Tyr Ala Glu Ile Asp His Ile Leu Pro Leu Ser Gln Ser Gln Asp Asp 
            580                 585                 590         


Ser Tyr Val Asn Lys Val Leu Val His Ser Ala Cys Asn Gln Asn Lys 
        595                 600                 605             


Lys Asn Arg Thr Pro Phe Glu Trp Phe Gln Asp Glu Lys Lys Asp Trp 
    610                 615                 620                 


Asp Thr Phe Lys Ser Tyr Ile Leu Met Glu Ser Thr Leu Gly Glu Lys 
625                 630                 635                 640 


Lys Arg Asn Tyr Leu Ile Lys Glu Asn Phe Ser Asp Pro Gln Ser Arg 
                645                 650                 655     


Lys Glu Phe Ile Ser Arg Asn Leu Asn Asp Thr Arg Tyr Met Ser Lys 
            660                 665                 670         


Ala Ile Lys Thr Tyr Cys Glu Asn His Trp Lys Leu Ser His Asp Asp 
        675                 680                 685             


Asp Lys Leu Arg Ile Gln Val Arg Ser Gly Lys Leu Thr Ser Thr Leu 
    690                 695                 700                 


Arg His Gln Trp Gly Leu Asp Asn Lys Asn Arg Glu Thr His Thr His 
705                 710                 715                 720 


His Ala Met Asp Ala Ile Met Ile Ala Phe Ser Thr Gln Gly Met Val 
                725                 730                 735     


Lys Lys Leu Ser Asp Tyr Phe Ala Lys Lys Glu Ala Lys Val Glu Lys 
            740                 745                 750         


Asp Lys Pro Val Leu Ile Thr Pro Ile Lys Gln Phe Lys Glu Ala Val 
        755                 760                 765             


Glu Gln Ala Thr Thr Leu Glu Arg Gln Glu Ser Ile Gln Thr Lys Ala 
    770                 775                 780                 


Gly Asp Thr Ile Thr Leu Asn Arg Leu Leu Ile Ser Arg Pro Pro Arg 
785                 790                 795                 800 


Ala Ser Val Thr Gly Ala Ala His Glu Gln Thr Ala Lys Pro Tyr Pro 
                805                 810                 815     


Arg Ile Lys Pro Ile Lys Asn Lys Tyr Lys Arg Arg Arg Ile Pro Ile 
            820                 825                 830         


Asp Glu Asp Lys Phe Glu Leu Phe Arg Asn Asp Lys Val Ala Ser Gly 
        835                 840                 845             


Asn Asp Lys Asn Phe Tyr Asn Ser Ser Thr Ile Pro Arg Val Asp Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Asp Lys Tyr His Val Val Pro Ile Tyr Leu Ser Asp 
865                 870                 875                 880 


Met Thr Lys Ala Glu Val Pro Asn Lys Ser Leu Gly Thr Asn Pro Glu 
                885                 890                 895     


Gly Met Asp Glu Lys Tyr Phe Cys Phe Ser Val Phe Lys Asn Asp Leu 
            900                 905                 910         


Ile Glu Leu Glu Thr Lys Ala Thr Pro Lys Lys Pro Ser Lys Lys Leu 
        915                 920                 925             


Leu Gly Tyr Phe Lys Gln Leu Asn Gly Ala Asn Phe Ile Leu Asn Ser 
    930                 935                 940                 


Ile His Asn Gly Ile Ile Asp Gly Phe Val Cys Ser Pro Ile Thr Leu 
945                 950                 955                 960 


Phe Lys Gln Gln Lys Asp Met Cys Lys Lys Cys Leu Pro Glu Asp Arg 
                965                 970                 975     


Ala Ile Gly Asn Cys Ser Gln Glu Thr Leu Glu Phe Trp Glu Ala Glu 
            980                 985                 990         


Asn Ile Lys Val Pro Lys Lys Asp  Phe Glu Cys Asp Gln  Gly Ile Lys 
        995                 1000                 1005             


Phe Ala  Ile Ala Val Arg Lys  Tyr Thr Ile Asp Pro  Leu Gly Tyr 
    1010                 1015                 1020             


Tyr His  Glu Val Lys Gly Glu  Lys Leu Leu Gly Thr  Ile Pro Gln 
    1025                 1030                 1035             


Gly Ala  Lys Lys His Pro Lys  Arg Gln Lys 
    1040                 1045             


<210>  517
<211>  1049
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  517

Met Ile Asn Glu Arg Leu Thr Phe Gly Ile Asp Leu Gly Ile Gly Ser 
1               5                   10                  15      


Cys Gly Trp Ala Val Leu Arg His Ala Asp Ala Ala Gly Glu Pro Gly 
            20                  25                  30          


Val Ile Glu Gly Leu Gly Ser Trp Cys Phe Asp Val Pro Glu Thr Ser 
        35                  40                  45              


Lys Glu Arg Ile Pro Thr Asn Gln Val Arg Arg Ser Asn Arg Leu Leu 
    50                  55                  60                  


Arg Arg Val Ile Arg Arg Arg Arg Asn Arg Met Ala Glu Ile Arg Arg 
65                  70                  75                  80  


Leu Phe His Ala Gln Gly Leu Leu Pro Ser Ala Asp Glu Asp Ala Leu 
                85                  90                  95      


Lys Arg Pro Arg Leu Asp Pro Trp Glu Leu Arg Ala Arg Gly Leu Asp 
            100                 105                 110         


Gln Cys Leu Ser Ala Glu Glu Phe Ala Val Ala Leu Gly His Ile Ala 
        115                 120                 125             


Arg His Arg Gly Phe Arg Ser Ser Ala Lys Arg Lys Ala Ala Asn Thr 
    130                 135                 140                 


Ser Gly Glu Asp Ser Lys Met Leu Ser Ala Leu Leu Val Thr Arg Glu 
145                 150                 155                 160 


Arg Thr Ala Arg Tyr Arg Thr Val Gly Glu Met Phe Ala Arg Asp Pro 
                165                 170                 175     


Glu Tyr Ser Ala Arg Arg Arg Asn Arg Asp Gly Ile Phe Asp Arg Thr 
            180                 185                 190         


Val Ala Arg Asp Asp Leu Ile His Glu Val Gly Lys Leu Phe Glu Ala 
        195                 200                 205             


Gln Arg Ala Arg Gly Ser Ala Phe Ala Ser Pro Asp Leu Glu Ala Asp 
    210                 215                 220                 


Phe Asn Ala Ile Ala Phe Arg Gln Ala Glu Met Gln Asp Ser Glu Arg 
225                 230                 235                 240 


Leu Val Gly Asn Cys Pro Phe Glu Arg Asp Glu Lys Arg Ala Ala Arg 
                245                 250                 255     


Trp Ala Pro Ser Phe Glu Arg Phe Arg Leu Leu Thr Arg Leu Ile Asn 
            260                 265                 270         


Leu Arg Ile Ala Thr Ala Glu Gly Glu Arg Arg Phe Thr Pro Glu Asp 
        275                 280                 285             


Leu Ala Arg Val Ser Ala Glu Leu Gly Ala Thr Ala Lys Leu Pro Val 
    290                 295                 300                 


Ser Arg Val Arg Ala Leu Ile Gly Leu Leu Pro Asp Glu Arg Phe Thr 
305                 310                 315                 320 


Thr Ile Arg Val Glu Asp Glu Asp Arg Asp Ile Val Ala Arg Thr Gly 
                325                 330                 335     


Glu Ala Ala Pro Gly Thr Trp Arg Leu Arg Lys Thr Leu Gly Glu Ala 
            340                 345                 350         


Leu Trp Ala Glu Met Gln Ala Arg Pro Asp Gln Leu Asp Ala Ile Ala 
        355                 360                 365             


His Val Leu Ser Phe Phe Glu Ala Thr Ala Lys Ile Arg Ser Glu Leu 
    370                 375                 380                 


Asp Arg Leu Gly Leu Pro Ala Ala Val Ile Glu Ala Leu Asp Arg Gly 
385                 390                 395                 400 


Leu Glu Asp Gly Ala Phe Ala Lys Phe Lys Gly Ala Gly His Ile Ser 
                405                 410                 415     


Ala Lys Ala Cys Arg Asn Leu Leu Pro His Leu Gln Gln Gly His Arg 
            420                 425                 430         


Tyr Asp Gln Ala Cys Arg Leu Ala Gly Tyr Asp His Ala Ala Ser Thr 
        435                 440                 445             


Leu Ser His Thr Ala Gln Val Val Ser Lys Ala Gly Phe Asn Ala Leu 
    450                 455                 460                 


Val Thr Glu Leu Gly Glu Ser Ile Ala Asn Pro Ile Ala Arg Lys Ala 
465                 470                 475                 480 


Leu Thr Glu Gly Leu Lys Gln Ile Arg Ala Met Cys Ala Arg Trp Gly 
                485                 490                 495     


Leu Pro Gly Ala Ile His Ile Glu Leu Ala Arg Glu Val Gly Asn Ser 
            500                 505                 510         


Ile Glu Lys Arg Arg Glu Leu Glu Ser Gln Arg Glu Thr Thr Thr Lys 
        515                 520                 525             


Arg Arg Gln Thr Glu Arg Asp Glu Ile Arg Gly Leu Leu Ala Leu Glu 
    530                 535                 540                 


Asp Val Asp Asp Asp Thr Leu Leu Arg Tyr Arg Leu Trp Lys Glu Gln 
545                 550                 555                 560 


Gly Gly Lys Cys Leu Tyr Thr Gly Lys Asp Ile His Val Arg Gln Leu 
                565                 570                 575     


Ile Ala Thr Asp Asn Ser Val Gln Val Asp His Ile Leu Pro Trp Ser 
            580                 585                 590         


Arg Phe Gly Asp Asp Ser Phe Asn Asn Lys Thr Leu Cys Leu Ala Ser 
        595                 600                 605             


Ala Asn Gln Arg Lys Arg Gly Gln Thr Pro Trp Glu Trp Phe Ser Leu 
    610                 615                 620                 


Thr Gly Thr Pro Glu Ala Trp Glu Val Phe Ala Ser Arg Val Glu Thr 
625                 630                 635                 640 


Ser Lys Asp Leu Arg Gly Phe Lys Lys Arg Asn Leu Leu Leu Lys Asn 
                645                 650                 655     


Ala Asp Glu Val Ala Gln Arg Phe Arg Ser Arg Asn Leu Asn Asp Thr 
            660                 665                 670         


Arg Tyr Ala Ala Arg Leu Leu Ala Glu Ala Ala Lys Leu Phe Tyr Pro 
        675                 680                 685             


Ala Gly Asp Arg Gln Glu Lys Asp Gly Arg Arg Arg Val Leu Thr Arg 
    690                 695                 700                 


Pro Gly Pro Leu Thr Ala Ala Leu Arg His Ala Trp Gly Leu Glu Ser 
705                 710                 715                 720 


Leu Lys Lys Ala Asp Gly Lys Arg Val Ser Asp Asp Arg His His Ala 
                725                 730                 735     


Leu Asp Ala Leu Ala Val Ala Ala Ile Ser Glu Arg Glu Val Gln Arg 
            740                 745                 750         


Leu Thr Arg Ser Phe Gln Glu Trp Glu Gln Gln Gly Leu Ala Arg Pro 
        755                 760                 765             


Leu Arg His Val Ala Pro Pro Trp Pro Gly Phe Arg Gly Asp Val Leu 
    770                 775                 780                 


Ala Ala Tyr Ala Gln Ala Phe Val Ala Arg Pro Glu Arg Arg Arg Ala 
785                 790                 795                 800 


Arg Gly Lys Gly His Asp Ala Ser Ile Arg Gln Val Val Gly Glu Gly 
                805                 810                 815     


Pro Glu Ala Gln Val Phe Glu Arg Arg Gly Leu Ala Asp Leu Lys Glu 
            820                 825                 830         


Ala Asp Leu Asp Arg Val Lys Asp Pro Glu Arg Asn Gln Ala Ile Ile 
        835                 840                 845             


Glu Ala Val Arg Ala Trp Ile Leu Ala Gly Arg Pro Ala Asp Ala Pro 
    850                 855                 860                 


Pro Arg Ser Pro Arg Gly Asp Val Ile Val Lys Leu Arg Thr Ala Pro 
865                 870                 875                 880 


Ala Lys Lys Ile Lys Pro Ala Val Pro Val Arg Gly Gly Thr Ala Asp 
                885                 890                 895     


Arg Gly Asp Met Val Arg Ile Asp Val Phe Thr Arg Pro Asn Arg Lys 
            900                 905                 910         


Gly Arg Asp Glu Trp Tyr Leu Val Pro Ile Tyr Pro His Gln Val Met 
        915                 920                 925             


Asn Arg Lys Ala Trp Pro His Pro Pro Met Arg Ser Val Val Ala Tyr 
    930                 935                 940                 


Lys Asp Glu Ala Gly Trp Thr Glu Ile Gly Ala Glu His Ser Phe Arg 
945                 950                 955                 960 


Phe Ser Leu Tyr Pro Arg Ser Tyr Val Gln Leu Val Lys Ser Asn Gly 
                965                 970                 975     


Asp Val Ile Glu Gly Tyr Leu Val Ser Met Asp Arg Ser Thr Gly Ala 
            980                 985                 990         


Phe Arg Val Phe Val His Asn Asn  Pro Ala Glu Met Ala  Lys Gly Ile 
        995                 1000                 1005             


Gly Ala  Lys Thr Leu Leu Asn  Leu Arg Lys Phe Asn  Val Asp Arg 
    1010                 1015                 1020             


Phe Gly  Val Arg Thr Glu Val  Lys Ser Glu Val Arg  Thr Trp His 
    1025                 1030                 1035             


Gly Glu  Val Cys Thr Ser Pro  Val Pro Pro Asp 
    1040                 1045                 


<210>  518
<211>  1049
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  518

Met Gln Leu Ser Lys Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ser Val Ile Glu Ile Asp Glu Thr Glu Met Pro 
            20                  25                  30          


Ile Arg Leu Ile Asp Val Gly Val Arg Thr Phe Glu Arg Ala Glu Val 
        35                  40                  45              


Pro Lys Thr Gly Glu Ser Leu Ala Leu Ala Arg Arg Leu Ala Arg Ser 
    50                  55                  60                  


Thr Arg Arg Leu Ile Arg Arg Arg Ala Phe Arg Leu Leu Lys Ala Lys 
65                  70                  75                  80  


Arg Leu Leu Lys Asn His Gln Ile Val Ser Ala Asp Glu Leu Ser Thr 
                85                  90                  95      


Leu Pro Asn His Cys Trp Glu Leu Arg Val Lys Gly Leu Asp Glu Arg 
            100                 105                 110         


Leu Thr Cys Gln Glu Trp Ala Ala Val Leu Leu His Leu Leu Lys His 
        115                 120                 125             


Arg Gly Tyr Leu Ser Gln Arg Lys Ser Glu Ala Gln Asn Ala Asp Lys 
    130                 135                 140                 


Glu Met Gly Ala Leu Leu Ser Gly Val Ala Asn Asn His Lys Leu Leu 
145                 150                 155                 160 


Ser Glu Asn His Tyr Arg Thr Pro Ala Glu Ile Ala Val Lys Lys Phe 
                165                 170                 175     


Ala Leu Glu Glu Gly His Met Arg Asn Gln Arg Gly Ala Tyr Thr His 
            180                 185                 190         


Thr Phe Asn Arg Leu Asp Leu Leu Ala Glu Met Arg Leu Leu Phe Gln 
        195                 200                 205             


Thr Gln Arg Glu Leu Gly Ser Pro Tyr Ala Ser Gly Glu Leu Glu Gln 
    210                 215                 220                 


Glu Phe Cys Lys Leu Leu Met Trp Gln Lys Pro Ala Leu Ser Gly Asp 
225                 230                 235                 240 


Ala Ile Leu Lys Met Leu Gly Lys Cys Thr Leu Glu Pro Thr Glu Tyr 
                245                 250                 255     


Lys Ala Ala Lys Asn Thr Tyr Thr Ala Glu Arg Phe Val Trp Leu Thr 
            260                 265                 270         


Lys Leu Asn Asn Leu Arg Ile Leu Glu Asn Gly Ser Glu Arg Ala Leu 
        275                 280                 285             


Asn Ala Asp Glu Arg Ala Leu Leu Leu Asn Gln Pro Tyr Glu Lys Ala 
    290                 295                 300                 


Lys Phe Thr Tyr Glu Gln Ala Arg Lys Leu Leu Gln Leu Asn Glu Ala 
305                 310                 315                 320 


Ala Arg Phe Asn Ser Val Arg Tyr Gly Lys Glu Asn Ala Glu Lys Ser 
                325                 330                 335     


Thr Leu Met Glu Met Lys Ala Tyr His Ala Ile Arg Lys Ala Val Glu 
            340                 345                 350         


Thr Ala Asp Ser Ser Ala Trp Ala Thr Leu Lys Thr Gln Pro Leu Leu 
        355                 360                 365             


Leu Asp Glu Ile Gly Thr Ala Phe Ser Ile Tyr Lys Thr Asp Glu Asp 
    370                 375                 380                 


Ile Lys Thr Gln Leu Gln Gly Lys Leu Ala Asp Asn Val Ile Asp Val 
385                 390                 395                 400 


Leu Leu Glu Asn Leu Asn Phe Asp Lys Phe Ile Gln Leu Ser Leu Lys 
                405                 410                 415     


Ala Leu Tyr Lys Ile Leu Pro Leu Met Glu Gln Gly Leu Arg Tyr Asp 
            420                 425                 430         


Glu Ala Cys Ser Gln Ile Tyr Gly Asp His Phe Gly Lys Lys Glu Gln 
        435                 440                 445             


Lys Asn Asp Arg Leu Leu Pro Gln Ile Pro Ala Asp Glu Ile Arg Asn 
    450                 455                 460                 


Pro Val Val Leu Arg Thr Leu Ser Gln Ala Arg Lys Val Ile Asn Ala 
465                 470                 475                 480 


Val Ile Arg Leu Tyr Gly Ser Pro Ala Arg Ile His Ile Glu Thr Ala 
                485                 490                 495     


Arg Glu Leu Gly Lys Ser Tyr Lys Asp Arg Lys Glu Ile Glu Lys Arg 
            500                 505                 510         


Gln Glu Gln Asn Arg Ala Glu Arg Glu Lys Ala Val Asp Lys Phe Lys 
        515                 520                 525             


Glu Leu Phe Pro Asn Phe Ile Gly Glu Pro Lys Ala Lys Asp Ile Leu 
    530                 535                 540                 


Thr Met Arg Leu Tyr Glu Gln Gln Asn Gly Gln Cys Leu Tyr Ser Gly 
545                 550                 555                 560 


Lys Ser Leu Asp Leu His Arg Leu Leu Glu Lys Gly Tyr Val Glu Ile 
                565                 570                 575     


Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn 
            580                 585                 590         


Lys Val Leu Val Leu Ala Gly Glu Asn Gln Asn Lys Ser Asn Gln Thr 
        595                 600                 605             


Pro Phe Glu Trp Leu Gly Lys Asp Glu Gln Lys Trp Ala Leu Phe Thr 
    610                 615                 620                 


Ala Arg Val Asn Ser Cys Arg Phe Pro Tyr Ser Lys Lys Gln Arg Ile 
625                 630                 635                 640 


Leu Thr Lys Lys Leu Asp Asp Glu Gly Phe Ile Gln Arg Asn Leu Asn 
                645                 650                 655     


Asp Thr Arg Tyr Ile Ala Arg Phe Leu Val Asn Phe Val Asn Asp Asn 
            660                 665                 670         


Leu His Leu Thr Gly Lys Gly Lys Arg Arg Val Phe Ala Ser Asn Gly 
        675                 680                 685             


Gln Ile Thr Ala Phe Leu Arg Gly Arg Trp Gly Leu Thr Lys Val Arg 
    690                 695                 700                 


Glu Glu Ser Asp Arg His His Ala Leu Asp Ala Val Val Val Ala Cys 
705                 710                 715                 720 


Ser Thr Val Ser Ile Gln Gln Lys Ile Thr Arg Phe Val Lys Tyr Gln 
                725                 730                 735     


Glu Gly Asn Leu Gln Thr Ala Glu Arg Ile Asp Arg Glu Thr Gly Glu 
            740                 745                 750         


Ile Ile Pro Leu His Phe Pro Asp Pro Trp Ala Phe Phe Arg Glu Glu 
        755                 760                 765             


Val Met Ile Arg Ile Phe Ser Glu Met Pro Gln Glu Asp Leu Val Ile 
    770                 775                 780                 


Lys Leu Pro Asp Arg Pro Gln Ala Asn His Glu Phe Val Gln Pro Leu 
785                 790                 795                 800 


Phe Val Ser Arg Met Pro Thr Arg Lys Met Ser Gly Gln Gly His Met 
                805                 810                 815     


Glu Thr Ile Lys Ser Ala Lys Arg Leu Asp Glu Gly Ile Ser Val Ser 
            820                 825                 830         


Lys Val Pro Leu Thr Gln Leu Lys Leu Lys Asp Leu Glu Asn Met Val 
        835                 840                 845             


Asn Arg Asp Arg Glu Ile Ala Leu Tyr Glu Ala Leu Lys Ala Arg Leu 
    850                 855                 860                 


Thr Glu Phe Lys Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr 
865                 870                 875                 880 


Lys Lys Gly Gly Val Gln Val Lys Ser Val Arg Val Glu Gln Val Gln 
                885                 890                 895     


Lys Ser Gly Val Val Val Arg Asn Gly Asn Gly Val Ala Asp Asn Ala 
            900                 905                 910         


Ser Met Val Arg Val Asp Val Phe Ile Lys Gly Gly Lys Tyr Phe Leu 
        915                 920                 925             


Val Pro Ile Tyr Thr Trp Gln Val Ala Lys Gly Ile Leu Pro Asp Arg 
    930                 935                 940                 


Ala Val Ile Ala Tyr Thr Pro Glu Asn Glu Trp Glu Leu Met Asp Asp 
945                 950                 955                 960 


Ser Ala Gln Phe Gln Phe Ser Ile Tyr Pro Asn Asp Leu Ile Lys Leu 
                965                 970                 975     


Thr Thr Lys Lys Ala Ile Phe Phe Gly Tyr Tyr Ser Gly Phe Asn Arg 
            980                 985                 990         


Leu Thr Gly Ala Ile Asn Ile Lys  Glu His Asp Leu Gln  Ile Ser Lys 
        995                 1000                 1005             


Gly Asp  Lys Gly Val His Leu  Gly Leu Gly Val Lys  Leu Ala Leu 
    1010                 1015                 1020             


Ser Leu  Glu Lys Tyr Gln Val  Asp Glu Leu Gly Lys  Asn Ile Arg 
    1025                 1030                 1035             


Pro Cys  Arg Pro Thr Gln Arg  Gln Asn Val Arg 
    1040                 1045                 


<210>  519
<211>  1050
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  519

Met Ser Ser Tyr Arg Phe Ser Phe Asp Leu Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Val Phe Glu Leu Asp Pro Lys Thr Arg Pro Val Ala Leu Ala 
            20                  25                  30          


Asp Cys Gly Val Arg Ile Phe Pro Asp Gly Arg Asn Pro Lys Asp Lys 
        35                  40                  45              


Gln Ser Leu Ala Val Ala Arg Arg Glu Ala Arg Ala Val Arg Arg Arg 
    50                  55                  60                  


Arg Asp Arg Tyr Leu Lys Arg Arg Ser Glu Leu Met Glu Arg Leu Val 
65                  70                  75                  80  


Ala Lys Gly Leu Met Pro Glu Ser Arg Asp Asp Gln Lys Ala Leu Glu 
                85                  90                  95      


Gly Leu Asp Pro Tyr Ala Leu Arg Ala Ala Gly Leu Asp Asp Ala Leu 
            100                 105                 110         


Pro Pro His His Phe Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg 
        115                 120                 125             


Gly Phe Lys Ser Asn Arg Lys Thr Asp Arg Gly Glu Asn Ser Glu Thr 
    130                 135                 140                 


Gly Ile Thr Lys Ile Gly Met Lys Lys Leu His Glu Ala Leu Leu Ser 
145                 150                 155                 160 


Glu Gly Cys Arg Thr Leu Gly Glu Leu Gln His Arg His His Glu Ala 
                165                 170                 175     


Asn Gln Thr Pro Ala Met Asp Thr Asn Pro Lys Ser Val Arg Ser Lys 
            180                 185                 190         


Leu Arg Gly Glu Gly Ala Lys Ala Val Tyr Asp Phe Tyr Pro Ser Arg 
        195                 200                 205             


Asp Ala Val Glu Asp Glu Phe Asn Lys Leu Trp Asp Ser Gln Lys Arg 
    210                 215                 220                 


Phe His Pro Asp Leu Leu Thr Asp Ser Ala Arg Asp Ala Ile Arg Asp 
225                 230                 235                 240 


Ala Ile Phe Tyr Gln Arg Lys Leu Lys Thr Pro Asp Ala Gly Arg Cys 
                245                 250                 255     


Ser Phe Asn Pro Ala Glu Glu Arg Val Ala Arg Ala Met Pro Ile Ala 
            260                 265                 270         


Gln His Phe Arg Ile Leu Gln Phe Val Gly Asp Leu Arg Leu Ile Asp 
        275                 280                 285             


Asn Asp Leu Val Arg His Pro Leu Asn Asn Asp Gln Lys Lys Ala Leu 
    290                 295                 300                 


Tyr Arg Asp Leu Leu Ala Lys Gly Lys Leu Thr Phe Lys Ala Met Arg 
305                 310                 315                 320 


Lys Thr Ile Gly Ile Gly Ser Glu Tyr Ala Phe Ser His Glu Ser Glu 
                325                 330                 335     


Lys Gln Ser Glu Phe Arg Gly Asp Glu Thr Ala Ala Arg Leu Ala Gly 
            340                 345                 350         


Pro Lys Tyr Phe Gly Pro Lys Trp Arg Glu Leu Ser Phe Asn Asp Gln 
        355                 360                 365             


Ser Glu Ile Val Asp Arg Leu Leu His Glu Glu Asn Glu Glu Thr Leu 
    370                 375                 380                 


Ile Gln Tyr Leu Gln Asp Arg Phe Gly Phe Asp His Asp Arg Cys Glu 
385                 390                 395                 400 


Val Ile Ala Asp Ala Pro Leu Pro Asp Gly His Thr Arg Leu Gly Lys 
                405                 410                 415     


Thr Ala Leu Glu Thr Leu Val Glu Ile Met Arg Asp Asp His Leu Pro 
            420                 425                 430         


Tyr Asp Gln Ala Cys Thr Lys Ala Gly Tyr Glu His Ala Leu Pro Pro 
        435                 440                 445             


Asp Gly Val Ile Leu Asp Asp Leu Pro Tyr Tyr Gly Ile Ala Leu Glu 
    450                 455                 460                 


Arg His Val Ala Phe Gly Asp Gly Lys Ser Glu Asn Pro Glu Lys Arg 
465                 470                 475                 480 


Tyr Gly Lys Ile Ala Asn Pro Thr Val His Ile Ala Leu Asn Gln Leu 
                485                 490                 495     


Arg Lys Val Ile Asn Ala Leu Ile Ala Arg Tyr Gly His Pro Asp Gln 
            500                 505                 510         


Val Val Met Glu Ile Ala Arg Asp Leu Lys Gln Ser Lys Ala Lys Arg 
        515                 520                 525             


Asp Asp Ile Ser Lys Glu Gln Lys Lys Asn Gln Asp Arg Asn Asp Gln 
    530                 535                 540                 


Arg Arg Lys Lys Leu Gln Glu Ala His Ile Ala Val Asn Ala Glu Asn 
545                 550                 555                 560 


Met Leu Arg Met Arg Leu Trp Glu Glu Leu Asp Glu Asp Asn Ala Ala 
                565                 570                 575     


Ala Arg Ala Cys Pro Phe Thr Gly Glu Thr Ile Ser Met Ala Arg Leu 
            580                 585                 590         


Phe Ser Pro Glu Val Glu Ile Glu His Ile Leu Pro Phe Ser Arg Ser 
        595                 600                 605             


Leu Asp Asn Ser Pro Ala Asn Lys Thr Leu Cys Met Val Trp Ala Asn 
    610                 615                 620                 


Arg Ala Lys Gly Asn Arg Thr Pro Trp Glu Ala Phe Gly Arg Asp Arg 
625                 630                 635                 640 


Glu Pro Gly Phe Asp Trp Pro Glu Ile Ala Ala Arg Ala Ala Arg Leu 
                645                 650                 655     


Pro Lys Asn Lys Ser Trp Arg Phe Gly Pro Asp Ala Met Glu Arg Leu 
            660                 665                 670         


Glu Arg Glu Gly Asp Phe Leu Asp Arg Gln Leu Thr Asp Thr Gln Tyr 
        675                 680                 685             


Leu Ser Arg Ile Ala Arg Glu Tyr Val Thr Lys Ile Cys Asp Pro Asn 
    690                 695                 700                 


Gln Val Trp Val Thr Pro Gly Arg Leu Thr Ala Trp Leu Arg Arg Asn 
705                 710                 715                 720 


Trp Gly Leu Asn Gly Ile Leu Pro Thr Gly Asn Ala Thr Lys Asn Arg 
                725                 730                 735     


Asp Asp His Arg His His Ala Ile Asp Ala Ile Val Val Gly Leu Thr 
            740                 745                 750         


Asp Arg Gly Ile Leu Asn Arg Val Ser Arg Ser Ala Ala Leu Ala Glu 
        755                 760                 765             


Ala Glu Asp Leu Asp Arg Ser Ile Ala Glu Met Pro Asp Pro Phe Asp 
    770                 775                 780                 


Gly Phe Arg Asp Asn Ile Arg His Ile Leu Glu Gln Ile Ile Val Ser 
785                 790                 795                 800 


His Lys Pro Asp Ala Gly Phe Val Pro Gly His Pro Lys Gly Asn Thr 
                805                 810                 815     


Thr Pro Gly Ala Leu His Asn Asp Thr Ala Tyr Gly Ile Ile Lys Thr 
            820                 825                 830         


Asp Ala Lys Gly Met Ser Glu Val Val Thr Arg Lys Asp Val Leu Ser 
        835                 840                 845             


Phe Lys Gly Thr Lys Glu Ile Glu Ser Ile Arg Asp Ala Ala Thr Arg 
    850                 855                 860                 


Gln Ser Leu Leu Asp Ala Val Ala Gly Val Pro Ser Gly Leu Ala Phe 
865                 870                 875                 880 


Lys Glu Ala Leu Arg Asp Trp Ser Val Arg Thr Gly Thr Lys Arg Val 
                885                 890                 895     


Arg Val Thr Phe Asn Met Ser Val Ile Pro Ile Gln Asp Arg Asp Gly 
            900                 905                 910         


Lys Ala Tyr Lys Gly Tyr Lys Gly Asp Gly Asn Leu Cys Ala Glu Ile 
        915                 920                 925             


Tyr Glu Arg Pro Asp Gly Lys Trp Gly Asp Glu Ile Ile Ser Arg Phe 
    930                 935                 940                 


Asp Ala Val Gln Pro Gly Phe Thr Pro Gln Trp Gln Arg Asp His Pro 
945                 950                 955                 960 


Ala Ala His Arg Val Met Arg Leu Phe Arg Asp Asp Met Ile Met Ala 
                965                 970                 975     


Glu Ile Asp Gly Val Met Thr Val Cys Arg Ile Val Lys Met Ser Pro 
            980                 985                 990         


Gly Lys Val Val Phe Thr Pro His  His Glu Ala Asn Thr  Asp Ser Arg 
        995                 1000                 1005             


Asn Arg  Asp Lys Asp Asp Pro  Phe Ser Phe His Thr  Cys Ser Gly 
    1010                 1015                 1020             


Glu Ser  Leu Arg Lys Arg Lys  Ala Gln Lys Leu Phe  Val Asp Pro 
    1025                 1030                 1035             


Leu Gly  Lys Ile Arg Gly Tyr  Arg Ala Lys Glu Gly  
    1040                 1045                 1050 


<210>  520
<211>  1050
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  520

Met Gly Glu Asn Met Ile Asp Glu Ser Leu Thr Phe Gly Ile Asp Leu 
1               5                   10                  15      


Gly Ile Gly Ser Cys Gly Trp Ala Val Leu Arg Arg Pro Ser Ala Phe 
            20                  25                  30          


Gly Arg Lys Gly Val Ile Glu Gly Met Gly Ser Trp Cys Phe Asp Val 
        35                  40                  45              


Pro Glu Thr Ser Lys Glu Arg Thr Pro Thr Asn Gln Ile Arg Arg Ser 
    50                  55                  60                  


Asn Arg Leu Leu Arg Arg Val Ile Arg Arg Arg Arg Asn Arg Met Ala 
65                  70                  75                  80  


Ala Ile Arg Arg Leu Leu His Ala Ala Gly Leu Leu Pro Ser Thr Asp 
                85                  90                  95      


Ser Asp Ala Leu Lys Arg Pro Gly His Asp Pro Trp Glu Leu Arg Ala 
            100                 105                 110         


Arg Gly Leu Asp Lys Pro Leu Lys Pro Val Glu Phe Ala Val Val Leu 
        115                 120                 125             


Gly His Ile Ala Lys Arg Arg Gly Phe Lys Ser Ala Ala Lys Arg Lys 
    130                 135                 140                 


Ala Thr Asn Ile Ser Ser Asp Asp Lys Lys Met Leu Thr Ala Leu Glu 
145                 150                 155                 160 


Ala Thr Arg Glu Arg Leu Gly Arg Tyr Arg Thr Val Gly Glu Met Phe 
                165                 170                 175     


Ala Arg Asp Pro Asp Phe Ala Ser Arg Arg Arg Asn Arg Glu Gly Lys 
            180                 185                 190         


Tyr Asp Arg Thr Thr Ala Arg Asp Asp Leu Glu His Glu Val His Ala 
        195                 200                 205             


Leu Phe Ala Ala Gln Arg Arg Leu Gly Gln Gly Phe Ala Ser Pro Glu 
    210                 215                 220                 


Leu Glu Glu Ala Phe Thr Ala Ser Ala Phe His Gln Arg Pro Met Gln 
225                 230                 235                 240 


Asp Ser Glu Arg Leu Val Gly Phe Cys Pro Phe Glu Arg Thr Glu Lys 
                245                 250                 255     


Arg Ala Ala Lys Leu Thr Pro Ser Phe Glu Arg Phe Arg Leu Leu Ala 
            260                 265                 270         


Arg Leu Leu Asn Leu Arg Ile Thr Thr Pro Asp Gly Glu Arg Pro Leu 
        275                 280                 285             


Thr Val Asp Glu Ile Ala Leu Val Thr Arg Asp Leu Gly Lys Thr Ala 
    290                 295                 300                 


Lys Leu Ser Ile Lys Arg Val Arg Thr Leu Ile Gly Leu Glu Asp Asn 
305                 310                 315                 320 


Gln Arg Phe Thr Thr Ile Arg Pro Glu Asp Glu Asp Arg Asp Ile Val 
                325                 330                 335     


Ala Arg Thr Gly Gly Ala Met Thr Gly Thr Ala Thr Leu Arg Lys Ala 
            340                 345                 350         


Leu Gly Glu Ala Leu Trp Thr Asp Met Gln Glu Arg Pro Glu Gln Leu 
        355                 360                 365             


Asp Ala Ile Val Gln Val Leu Ser Phe Phe Glu Ala Asn Glu Thr Ile 
    370                 375                 380                 


Thr Glu Lys Leu Arg Glu Ile Gly Leu Thr Leu Ala Val Leu Asp Val 
385                 390                 395                 400 


Leu Leu Thr Ala Leu Asp Ala Gly Val Phe Ala Lys Phe Lys Gly Ala 
                405                 410                 415     


Ala His Ile Ser Thr Lys Ala Ala Arg Asn Leu Leu Pro His Leu Glu 
            420                 425                 430         


Gln Gly Arg Arg Tyr Asp Glu Ala Cys Thr Met Ala Gly Tyr Asp His 
        435                 440                 445             


Ala Ala Ser Arg Leu Ser His His Gly Gln Ile Val Ala Lys Thr Gln 
    450                 455                 460                 


Phe Asn Ala Leu Val Thr Glu Ile Gly Glu Ser Ile Ala Asn Pro Ile 
465                 470                 475                 480 


Ala Arg Lys Ala Leu Ile Glu Gly Leu Lys Gln Ile Trp Ala Met Arg 
                485                 490                 495     


Asn His Trp Gly Leu Pro Gly Ser Ile His Val Glu Leu Ala Arg Asp 
            500                 505                 510         


Val Gly Asn Ser Ile Glu Lys Arg Arg Glu Ile Glu Lys His Ile Glu 
        515                 520                 525             


Lys Asn Thr Ala Leu Arg Ala Arg Glu Arg Arg Glu Val His Asp Leu 
    530                 535                 540                 


Leu Asp Leu Glu Asp Val Asn Gly Asp Thr Leu Leu Arg Tyr Arg Leu 
545                 550                 555                 560 


Trp Lys Glu Gln Gly Gly Lys Cys Leu Tyr Thr Gly Lys Ala Ile His 
                565                 570                 575     


Ile Arg Gln Ile Ala Ala Thr Asp Asn Ser Val Gln Val Asp His Ile 
            580                 585                 590         


Leu Pro Trp Ser Arg Phe Gly Asp Asp Ser Phe Asn Asn Lys Thr Leu 
        595                 600                 605             


Cys Leu Ala Ser Ala Asn Gln Gln Lys Lys Arg Ser Thr Pro Tyr Glu 
    610                 615                 620                 


Trp Leu Ser Gly Gln Thr Gly Asp Ala Trp Asn Ala Phe Val Gln Arg 
625                 630                 635                 640 


Ile Glu Thr Asn Lys Glu Leu Arg Gly Phe Lys Lys Arg Asn Tyr Leu 
                645                 650                 655     


Leu Lys Asn Ala Lys Glu Ala Glu Glu Lys Phe Arg Ser Arg Asn Leu 
            660                 665                 670         


Asn Asp Thr Arg Tyr Ala Ala Arg Leu Phe Ala Glu Ala Val Lys Leu 
        675                 680                 685             


Leu Tyr Ala Phe Gly Glu Arg Gln Glu Lys Gly Gly Asn Arg Arg Val 
    690                 695                 700                 


Phe Thr Arg Pro Gly Ala Leu Thr Ala Ala Leu Arg Gln Ala Trp Gly 
705                 710                 715                 720 


Val Glu Ser Leu Lys Lys Gln Asp Gly Lys Arg Ile Asn Asp Asp Arg 
                725                 730                 735     


His His Ala Leu Asp Ala Leu Thr Val Ala Ala Val Asp Glu Ala Glu 
            740                 745                 750         


Ile Gln Arg Leu Thr Lys Ser Phe His Glu Trp Glu Gln Gln Gly Leu 
        755                 760                 765             


Gly Arg Pro Leu Arg Arg Val Glu Pro Pro Trp Glu Ser Phe Arg Ala 
    770                 775                 780                 


Asp Val Glu Ala Thr Tyr Pro Glu Val Phe Val Ala Arg Pro Glu Arg 
785                 790                 795                 800 


Arg Arg Ala Arg Gly Glu Gly His Ala Ala Thr Ile Arg Gln Val Lys 
                805                 810                 815     


Glu Arg Glu Cys Thr Pro Ile Val Phe Glu Arg Lys Ala Val Ser Ser 
            820                 825                 830         


Leu Lys Glu Ala Asp Leu Glu Arg Ile Lys Asp Gly Glu Arg Asn Glu 
        835                 840                 845             


Ala Ile Val Glu Ala Ile Arg Ser Trp Ile Ala Thr Gly Arg Pro Ala 
    850                 855                 860                 


Asp Ala Pro Pro Arg Ser Pro Arg Gly Asp Ile Ile Thr Lys Ile Arg 
865                 870                 875                 880 


Leu Ala Thr Thr Ile Lys Ala Ala Val Pro Val Arg Gly Gly Thr Ala 
                885                 890                 895     


Gly Arg Gly Glu Met Val Arg Ala Asp Val Phe Ser Lys Pro Asn Arg 
            900                 905                 910         


Arg Gly Lys Asp Glu Trp Tyr Leu Val Pro Val Tyr Pro His Gln Ile 
        915                 920                 925             


Met Asn Arg Lys Ala Trp Pro Lys Pro Pro Met Arg Ser Ile Val Ala 
    930                 935                 940                 


Asn Lys Asp Glu Asp Glu Trp Thr Glu Val Gly Pro Glu His Gln Phe 
945                 950                 955                 960 


Arg Phe Ser Leu Tyr Pro Arg Ser Asn Ile Glu Ile Ile Arg Pro Ser 
                965                 970                 975     


Gly Glu Val Ile Glu Gly Tyr Phe Val Gly Leu His Arg Asn Thr Gly 
            980                 985                 990         


Ala Leu Thr Ile Ser Ala His Asn  Asp Pro Lys Ser Ile  His Ser Gly 
        995                 1000                 1005             


Ile Gly  Thr Lys Thr Leu Leu  Ala Ile Ser Lys Tyr  Gln Val Asp 
    1010                 1015                 1020             


Arg Phe  Gly Arg Lys Ser Pro  Val Arg Lys Glu Val  Arg Thr Trp 
    1025                 1030                 1035             


His Gly  Glu Ala Cys Ile Ser  Pro Thr Pro Pro Gly  
    1040                 1045                 1050 


<210>  521
<211>  1050
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  521

Met Lys Ile Leu Gly Phe Asp Ile Gly Ile Ala Ser Ile Gly Trp Ala 
1               5                   10                  15      


Phe Val Glu Asn Gly Glu Leu Lys Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Lys Thr Gly Asp Ser Leu Ala Met Pro Arg Arg 
        35                  40                  45              


Glu Ala Arg Ser Val Arg Arg Arg Leu Ala Arg Arg Lys Gly Arg Leu 
    50                  55                  60                  


Glu Thr Leu Lys Arg Leu Leu Ala Lys Glu Trp Asp Leu Cys Tyr Glu 
65                  70                  75                  80  


Asp Tyr Ile Ala Ala Asp Gly Glu Leu Pro Lys Ala Phe Met Gly Lys 
                85                  90                  95      


Asn Leu Asn Asn Pro Tyr Val Leu Arg Tyr Glu Ala Leu Gln Arg Leu 
            100                 105                 110         


Leu Ser Lys Glu Glu Leu Val Arg Val Val Leu His Ile Ala Lys His 
        115                 120                 125             


Arg Gly Tyr Gly Asn Lys Asn Ala Lys Ile Thr Lys Ser Glu Glu Ser 
    130                 135                 140                 


Lys Arg Glu Gln Gly Lys Ile Leu Ser Ala Leu Ala Thr Asn Ala Ser 
145                 150                 155                 160 


Ala Ile Ala Arg Tyr Arg Thr Val Gly Glu Tyr Phe Tyr Lys Glu Phe 
                165                 170                 175     


Cys Glu Val Ile Lys Asn Pro Gln Gly Leu Ser Thr Asn Glu His Tyr 
            180                 185                 190         


Thr Gln Ser Lys Val Arg Val Leu Lys Pro Ile Arg Asn Lys Gly Gly 
        195                 200                 205             


Glu Tyr Thr Asn Cys Ile Leu Gln Glu Asp Leu Gln Arg Glu Leu Arg 
    210                 215                 220                 


Cys Ile Phe Glu Tyr Gln Lys Gly Phe Gly Phe Pro Ile Thr Gln Glu 
225                 230                 235                 240 


Phe Gln Asp Lys Ile Leu Lys Ile Ala Phe Tyr Gln Arg Pro Leu Lys 
                245                 250                 255     


Asp Phe Ser His Leu Val Gly Lys Cys Thr Phe Tyr Pro Asp Glu Phe 
            260                 265                 270         


Arg Ala Leu Lys Cys Ser Leu Ser Ala Val Glu Phe Ile Thr Leu Ser 
        275                 280                 285             


Lys Thr Ile Asn Val Leu Thr Tyr Ile Ser Lys Glu Ser Gly Glu Val 
    290                 295                 300                 


Tyr Ser Lys Gly Gln Tyr Glu Gln Met Leu Asp Ser Val Phe Lys Ala 
305                 310                 315                 320 


Val Cys Glu Lys Gly Ser Leu Ser Phe Ala Gln Leu Arg Lys Ile Leu 
                325                 330                 335     


His Ile Asp Glu Arg Ile Arg Phe Lys Glu Val Asp Tyr Met Ala Arg 
            340                 345                 350         


Lys Ser Lys Asn Thr Ser Gly Asp Ser Thr His Lys Gln Lys Thr Ser 
        355                 360                 365             


Asn Pro Glu Thr Lys Lys Phe Ile Glu His Lys Asn Leu Lys Lys Phe 
    370                 375                 380                 


Cys Glu Ile Leu Ser Glu Ile Ala Glu Asp Arg Ala Leu Ala Asp Ser 
385                 390                 395                 400 


Ile Ala Arg Asp Met Thr Leu Ile Lys Asp Glu Glu Lys Leu Ala Gln 
                405                 410                 415     


Lys Leu His Gly Tyr Gly Lys Phe Ser Gln Glu Gln Ile Thr Gln Leu 
            420                 425                 430         


Ser Ala Leu Asn Phe Ser His His Ile Asn Leu Ser Leu Lys Ala Leu 
        435                 440                 445             


Ser Glu Ile Leu Pro Phe Met Arg Glu Gly Met Arg Tyr Asp Glu Ala 
    450                 455                 460                 


Cys Lys Leu Ala Gly Leu Lys Ala Arg His Asn Asp Lys Lys Ser Lys 
465                 470                 475                 480 


Phe Leu Pro Pro Phe Cys Glu Ser Ile Tyr Ala Asp Glu Leu Thr Asn 
                485                 490                 495     


Pro Val Val His Arg Ala Ile Ala Glu Tyr Arg Lys Ile Leu Asn Ala 
            500                 505                 510         


Leu Ile Ala Lys Tyr Gly Ser Val His Lys Ile His Ile Glu Leu Thr 
        515                 520                 525             


Arg Asp Val Gly Lys Asn Phe Gln Glu Arg Glu Lys Tyr Lys Lys Glu 
    530                 535                 540                 


Ile Glu Ser Asn Tyr Lys Ala Arg Val Gln Ala Met Gln Glu Cys Glu 
545                 550                 555                 560 


Lys Leu Gly Leu Thr Leu Ser Glu Gly Asn Ile Leu Lys Leu Arg Leu 
                565                 570                 575     


Phe Arg Glu Gln Asn Glu Ile Cys Val Tyr Ser Gly Arg Lys Ile Thr 
            580                 585                 590         


Leu Ala Asn Leu Lys Glu Gln Gly Ala Leu Glu Ile Asp His Ile Leu 
        595                 600                 605             


Pro Tyr Ser Arg Ser Ser Asp Asp Ser Tyr Met Asn Lys Val Leu Val 
    610                 615                 620                 


Phe Thr Asn Glu Asn Gln Asn Lys Gly Asn Lys Thr Pro Tyr Glu Ala 
625                 630                 635                 640 


Phe Gly Gly Asp Ser Gln Lys Trp Gly Glu Ile Glu Ser Leu Ala Leu 
                645                 650                 655     


Arg Ser Gly Tyr Pro Lys Lys Lys Ala Lys Arg Ile Leu Asp Lys Gly 
            660                 665                 670         


Phe Gly Asp Arg Glu Ala Gly Phe Lys Ser Arg Asn Ile Val Asp Ser 
        675                 680                 685             


Gly Tyr Ile Ala Arg Leu Ile Ala Asn Tyr Thr Lys Glu Tyr Leu Lys 
    690                 695                 700                 


Phe Leu Pro Leu Asp Ser His Glu Asn Thr Ala Leu Ile Ala Gly Glu 
705                 710                 715                 720 


Lys Asp Ser Lys Ile His Val Glu Ala Val Lys Gly Met Leu Thr Ala 
                725                 730                 735     


Thr Met Arg His Phe Trp Gly Leu Gly Ser Lys Asn Arg Tyr Glu His 
            740                 745                 750         


Thr His His Ala Val Asp Ala Ile Ile Ile Ala Tyr Ile Asn Ala Ala 
        755                 760                 765             


Met Ile Gln Arg Phe Ser Gln Phe Arg Gln Asn Gln Glu Ser Leu Lys 
    770                 775                 780                 


Ala Arg Phe Tyr Ala Lys Glu Leu Ala Lys Glu Glu Phe Lys Thr Gln 
785                 790                 795                 800 


Arg Ala Phe Phe Glu Pro Phe Arg Gly Phe Arg Glu Gln Val Leu Ala 
                805                 810                 815     


Lys Val Gly Gln Ile Phe Val Ser Arg Pro Pro Arg Lys Arg Ala Arg 
            820                 825                 830         


Gly Ala Leu His Glu Glu Thr Phe Tyr Ser Ile Asp Asp Lys Lys Leu 
        835                 840                 845             


Ser Glu Thr Tyr Gly Gly Lys Glu Gly Val Gln Arg Ala Leu Ser Leu 
    850                 855                 860                 


Gly Lys Ile Arg Gln Ile Gly Thr Lys Ile Val Ala Asn Gly Pro Met 
865                 870                 875                 880 


Val Arg Val Asp Ile Phe Lys His Lys Gln Ser Arg Arg Phe Tyr Ala 
                885                 890                 895     


Val Pro Ile Tyr Thr Met Asp Phe Ala Leu Gly Ile Leu Pro Asn Lys 
            900                 905                 910         


Ala Val Val Ser Gly Lys Asp Thr Lys Ser Lys Val Ile Lys Asp Trp 
        915                 920                 925             


Leu Glu Met Asp Ser Cys Tyr Glu Phe Cys Phe Ser Leu Phe Lys Asp 
    930                 935                 940                 


Asp Leu Ile Glu Val Gln Lys Ser Asp Met Glu Lys Pro Glu Leu Ala 
945                 950                 955                 960 


Tyr Phe Val Ser Leu Asp Ala Ser Asp Gly Arg Ile Lys Val Arg His 
                965                 970                 975     


His Cys Asn Asp Ile Thr Lys Ile Asn Glu Asn Gln Lys Lys Leu Phe 
            980                 985                 990         


Ser Glu Ala Ile Glu Lys Glu Val  Val Gly Arg Gly Ser  Ile Gln Asn 
        995                 1000                 1005             


Leu Lys  Val Phe Lys Lys Tyr  Lys Val Ser Pro Leu  Gly Glu Ile 
    1010                 1015                 1020             


Lys Glu  Ala Lys Tyr Glu Pro  Arg Gln Pro Ile Ala  Leu Lys Thr 
    1025                 1030                 1035             


Ser Pro  Lys Lys His Arg Lys  Gln Asn Asn Ala Gln  
    1040                 1045                 1050 


<210>  522
<211>  1051
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  522

Met Asn Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly 
1               5                   10                  15      


Tyr Gly Ile Val Asp Ser Asp Thr Arg Glu Ile Lys Asp Ala Gly Val 
            20                  25                  30          


Arg Leu Phe Pro Glu Ala Asn Val Asp Asn Asn Glu Gly Arg Arg Ser 
        35                  40                  45              


Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu Asp 
    50                  55                  60                  


Arg Val Lys His Leu Leu Ala Glu Tyr Asp Leu Leu Asp Leu Thr Asn 
65                  70                  75                  80  


Ile Pro Lys Ser Thr Asn Pro Tyr Gln Thr Arg Val Lys Gly Leu Asn 
                85                  90                  95      


Glu Lys Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile His Asn Val Asn Val Met Met Asp Asp Asn Asp 
        115                 120                 125             


Ser Gly Asn Glu Leu Ser Thr Lys Asp Gln Leu Lys Lys Asn Ala Lys 
    130                 135                 140                 


Ala Leu Ser Asp Lys Tyr Val Cys Glu Leu Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Gln Asp Tyr Lys Val Arg Gly Glu Lys Asn Arg Phe Lys Thr Glu Asp 
                165                 170                 175     


Phe Val Arg Glu Ala Arg Lys Leu Leu Glu Thr Gln Ser Lys Phe Phe 
            180                 185                 190         


Glu Ile Asp Gln Thr Phe Ile Met Arg Tyr Ile Glu Leu Ile Glu Thr 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro Phe Gly Trp 
    210                 215                 220                 


Glu Gly Asn Ile Lys Lys Trp Phe Glu Gln Met Met Gly His Cys Thr 
225                 230                 235                 240 


Tyr Phe Pro Glu Glu Leu Arg Ser Val Lys Tyr Ser Tyr Ser Ala Glu 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp 
            260                 265                 270         


Glu Asp Ala Lys Leu Asn Tyr Gly Glu Lys Phe Gln Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Thr Pro Asn Leu Lys Gln Ile Ala Ile Glu 
    290                 295                 300                 


Ile Gly Val His Glu Thr Glu Ile Lys Gly Tyr Arg Val Asn Lys Ser 
305                 310                 315                 320 


Gly Lys Pro Glu Phe Thr Gln Phe Lys Leu Tyr His Asp Leu Lys Asn 
                325                 330                 335     


Ile Phe Lys Asp Pro Lys Tyr Leu Asn Asp Ile Gln Leu Met Asp Asn 
            340                 345                 350         


Ile Ala Glu Ile Ile Thr Ile Tyr Gln Asp Ala Glu Ser Ile Ile Lys 
        355                 360                 365             


Glu Leu Asn Gln Leu Pro Glu Leu Leu Ser Glu Arg Glu Lys Glu Lys 
    370                 375                 380                 


Ile Ser Ala Leu Ser Gly Tyr Ser Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Cys Ile Asn Leu Leu Leu Asp Asp Leu Trp Glu Ser Ser Leu Asn Gln 
                405                 410                 415     


Met Glu Leu Phe Thr Lys Leu Asn Leu Lys Pro Lys Lys Ile Asp Leu 
            420                 425                 430         


Ser Gln Gln His Lys Ile Pro Ser Lys Leu Val Asp Asp Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Ala Phe Ile Gln Ser Ile Gln Val Val Asn 
    450                 455                 460                 


Ala Ile Ile Asp Lys Tyr Gly Leu Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Asp Asp Arg Arg Lys Phe Leu Asn Gln Leu 
                485                 490                 495     


Gln Lys Gln Asn Glu Glu Thr Arg Lys Gln Val Glu Lys Val Leu Arg 
            500                 505                 510         


Glu Tyr Gly Asn Asp Asn Ala Lys Arg Ile Val Gln Lys Ile Lys Leu 
        515                 520                 525             


His Asn Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Lys Asp Ile Pro 
    530                 535                 540                 


Leu Glu Asp Leu Leu Arg Asn Pro His His Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ala Phe Asp Asn Ser Met His Asn Lys Val Leu 
                565                 570                 575     


Val Arg Ala Asp Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Leu Asn Ser Ser Glu Ser Ser Leu Ser Tyr Asn Glu Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Thr Lys Asp Arg Ile Thr Lys Lys Lys 
    610                 615                 620                 


Arg Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Asp Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Ser Leu Leu Lys Ala Tyr Phe Ser Ala Asn Asn Leu Asp 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asn Tyr Leu Arg Lys 
        675                 680                 685             


Val Trp Lys Phe Asp Lys Asp Arg Asn Lys Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys His Asn 
705                 710                 715                 720 


Lys Lys Leu Arg Asn Ile Asn Lys Val Leu Asp Ala Pro Ser Lys Glu 
                725                 730                 735     


Val Asp Lys Lys Arg Val Thr Val Gln Ser Glu Asp Glu Tyr Asn Gln 
            740                 745                 750         


Ile Phe Glu Asp Thr Gln Lys Ala Gln Ala Ile Lys Lys Phe Glu Ile 
        755                 760                 765             


Arg Lys Phe Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Asn Ile Asp Gly Ile Glu Tyr Val 
785                 790                 795                 800 


Val Glu Ser Ile Lys Asp Ile Tyr Ser Val Asn Asn Asp Lys Val Lys 
                805                 810                 815     


Thr Lys Phe Lys Lys Asp Pro His Arg Leu Leu Met Tyr Arg Asn Asp 
            820                 825                 830         


Pro Gln Thr Phe Glu Lys Phe Glu Lys Val Phe Lys Gln Tyr Glu Ser 
        835                 840                 845             


Glu Lys Asn Pro Phe Ala Lys Tyr Tyr Glu Glu Thr Gly Glu Lys Ile 
    850                 855                 860                 


Arg Lys Phe Ser Lys Thr Gly Gln Gly Pro Tyr Ile Asn Lys Ile Lys 
865                 870                 875                 880 


Tyr Leu Arg Glu Arg Leu Gly Arg His Cys Asp Val Thr Asn Lys Tyr 
                885                 890                 895     


Ile Asn Ser Arg Asn Lys Ile Val Gln Leu Lys Ile Tyr Ser Tyr Arg 
            900                 905                 910         


Phe Asp Ile Tyr Gln Tyr Gly Asn Asn Tyr Lys Met Ile Thr Ile Ser 
        915                 920                 925             


Tyr Ile Asp Leu Glu Gln Lys Ser Asn Tyr Tyr Tyr Ile Ser Arg Glu 
    930                 935                 940                 


Lys Tyr Glu Gln Lys Lys Lys Asp Lys Gln Ile Asp Asp Ser Tyr Lys 
945                 950                 955                 960 


Phe Ile Gly Ser Phe Tyr Lys Asn Asp Ile Ile Asn Tyr Asn Gly Glu 
                965                 970                 975     


Met Tyr Arg Val Ile Gly Val Asn Asp Ser Glu Lys Asn Lys Ile Gln 
            980                 985                 990         


Leu Asp Met Ile Asp Ile Ser Ile  Lys Asp Tyr Met Glu  Leu Asn Asn 
        995                 1000                 1005             


Ile Lys  Lys Thr Gly Val Ile  Tyr Lys Thr Ile Gly  Lys Ser Thr 
    1010                 1015                 1020             


Thr His  Ile Glu Lys Tyr Thr  Thr Asp Ile Leu Gly  Asn Leu Tyr 
    1025                 1030                 1035             


Lys Ala  Ala Pro Pro Lys Lys  Pro Gln Leu Ile Phe  Lys 
    1040                 1045                 1050     


<210>  523
<211>  1052
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  523

Met Glu Asn Lys Asn Leu His Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ala Val Val Glu Ile Asp Glu Lys Glu Asn Pro 
            20                  25                  30          


Leu Arg Leu Ile Asp Val Gly Val Arg Thr Phe Glu Arg Ala Glu Thr 
        35                  40                  45              


Gln Lys Gly Glu Ser Leu Ala Leu Ser Arg Arg Ser Ala Arg Ser Ala 
    50                  55                  60                  


Arg Arg Leu Thr Gln Arg Arg Val Ala Arg Leu Lys Lys Ala Lys Arg 
65                  70                  75                  80  


Leu Leu Lys Ser Glu Asn Ile Leu Leu Ser Thr Asp Glu Arg Leu Pro 
                85                  90                  95      


His Gln Val Trp Gln Leu Arg Val Glu Gly Leu Asp Arg Lys Leu Glu 
            100                 105                 110         


Arg Gln Glu Trp Ala Ala Val Leu Leu His Leu Ile Lys His Arg Gly 
        115                 120                 125             


Tyr Leu Ser Gln Arg Lys Asn Glu Ser Lys Ser Glu Asn Lys Glu Leu 
    130                 135                 140                 


Gly Ala Leu Leu Ser Gly Val Ala Ser Asn His Glu Leu Leu Gln Gln 
145                 150                 155                 160 


Ala Thr Tyr Arg Thr Pro Ala Glu Leu Ala Val Lys Lys Phe Glu Val 
                165                 170                 175     


Glu Glu Gly His Ile Arg Asn Gln Gln Gly Ala Tyr Thr His Thr Phe 
            180                 185                 190         


Ser Arg Leu Asp Leu Leu Ala Glu Met Glu Leu Leu Phe Ser Arg Gln 
        195                 200                 205             


Gln His Phe Gly Asn Pro Phe Ala Ser Glu Lys Leu Leu Glu Asn Leu 
    210                 215                 220                 


Thr Ala Leu Leu Met Trp Gln Lys Pro Ala Leu Ser Gly Glu Asp Ile 
225                 230                 235                 240 


Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Asp Glu Tyr Lys Ala Ala 
                245                 250                 255     


Lys Asn Thr Tyr Thr Ala Glu Arg Phe Val Trp Ile Thr Lys Leu Asn 
            260                 265                 270         


Asn Leu Arg Ile Gln Glu Asn Gly Leu Glu Arg Ala Leu Ser Asp Asn 
        275                 280                 285             


Glu Arg Leu Met Leu Ile Glu Gln Pro Tyr Glu Lys Ala Lys Leu Thr 
    290                 295                 300                 


Tyr Ala Gln Val Arg Ser Ile Leu Asn Leu Ser Asp Asp Ala Ile Phe 
305                 310                 315                 320 


Lys Gly Val Arg Tyr Ser Gly Glu Asp Lys Lys Ala Ile Glu Thr Lys 
                325                 330                 335     


Thr Thr Leu Met Glu Met Lys Ala Tyr His Gln Ile Arg Lys Val Leu 
            340                 345                 350         


Glu Gly Asn Asn Leu Lys Ala Glu Trp Val Ala Leu Lys Ala Asn Pro 
        355                 360                 365             


Thr Leu Leu Asp Glu Ile Gly Thr Ala Phe Ser Leu Tyr Lys Thr Asp 
    370                 375                 380                 


Glu Asp Ile Ser Ala Tyr Leu Ala Gly Lys Leu Ser Gln Pro Val Leu 
385                 390                 395                 400 


Asn Ala Leu Leu Glu Asn Leu Ser Phe Asp Lys Phe Ile Gln Leu Ser 
                405                 410                 415     


Leu Lys Ala Leu Tyr Lys Leu Leu Pro Leu Met Gln Gln Gly Leu Arg 
            420                 425                 430         


Tyr Asp Glu Ala Cys Arg Glu Ile Tyr Gly Asp His Tyr Gly Lys Lys 
        435                 440                 445             


Thr Glu Glu Thr His His Phe Leu Pro Gln Ile Pro Ala Asp Glu Ile 
    450                 455                 460                 


Arg Asn Pro Val Val Leu Arg Thr Leu Thr Gln Ala Arg Lys Val Ile 
465                 470                 475                 480 


Asn Gly Val Val Arg Leu Tyr Gly Ser Pro Ala Arg Ile His Ile Glu 
                485                 490                 495     


Thr Gly Arg Glu Val Gly Lys Ser Tyr Lys Asp Arg His Glu Leu Lys 
            500                 505                 510         


Lys Arg Gln Glu Glu Asn Arg Lys Gln Arg Glu Lys Thr Ile Ser Glu 
        515                 520                 525             


Ile Lys Thr Leu Phe Pro Asn Phe Ser Gly Glu Pro Lys Gly Lys Asp 
    530                 535                 540                 


Ile Leu Lys Met Arg Leu Tyr Tyr Gln Gln Asn Ala Lys Cys Leu Tyr 
545                 550                 555                 560 


Ser Gly Lys Pro Leu Glu Leu His Arg Leu Phe Glu Gln Lys Tyr Val 
                565                 570                 575     


Glu Val Asp His Ala Leu Pro Leu Ser Arg Thr Trp Asp Asp Ser Phe 
            580                 585                 590         


Asn Asn Lys Val Leu Val Leu Ala Asn Glu Asn Gln Asn Lys Gly Asn 
        595                 600                 605             


Leu Thr Pro Phe Glu Trp Leu Asp Gly Lys Asn Asn Ser Glu Arg Trp 
    610                 615                 620                 


Arg Thr Phe Lys Ala Leu Val Glu Thr Ser Ala Phe Pro Tyr Ala Lys 
625                 630                 635                 640 


Lys Gln Arg Ile Leu Ser Gln Lys Leu Asp Glu Lys Gly Phe Ile Glu 
                645                 650                 655     


Arg Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe Leu Cys Asn Phe 
            660                 665                 670         


Ile Ala Asp Asn Met His Leu Thr Gly Glu Gly Lys Arg Lys Val Phe 
        675                 680                 685             


Ala Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg Arg Arg Trp Gly Leu 
    690                 695                 700                 


Ala Lys Ser Arg Glu Asp Asn Asp Arg His His Ala Leu Asp Ala Val 
705                 710                 715                 720 


Leu Val Ala Cys Ser Thr Val Ala Met Gln Gln Lys Ile Thr Arg Phe 
                725                 730                 735     


Val Arg Phe Lys Ala Gly Asp Val Phe Thr Gly Glu Arg Ile Asp Arg 
            740                 745                 750         


Glu Thr Gly Glu Ile Ile Pro Leu His Phe Pro Thr Pro Trp Gln Phe 
        755                 760                 765             


Phe Lys Gln Glu Val Glu Ile Arg Ile Phe Ser Asp Asn Pro Lys Leu 
    770                 775                 780                 


Glu Leu Glu Asn Arg Leu Pro Asp Arg Pro Gln Ala Asn His Glu Phe 
785                 790                 795                 800 


Val Gln Pro Leu Phe Val Ser Arg Met Pro Thr Arg Lys Met Thr Gly 
                805                 810                 815     


Gln Gly His Met Glu Thr Val Lys Ser Ala Lys Arg Leu Asn Glu Gly 
            820                 825                 830         


Ile Ser Met Ile Lys Met Pro Leu Thr Lys Leu Lys Leu Lys Asp Leu 
        835                 840                 845             


Glu Leu Met Val Asn Arg Glu Arg Glu Lys Asp Leu Tyr Asp Ala Leu 
    850                 855                 860                 


Lys Thr Arg Leu Glu Ala Phe Asn Asp Asp Pro Ala Lys Ala Phe Ala 
865                 870                 875                 880 


Glu Pro Phe Met Lys Lys Gly Gly Ala Ile Val Lys Ser Val Arg Val 
                885                 890                 895     


Glu Gln Val Gln Lys Ser Gly Val Leu Val Arg Gln Gly Asn Gly Val 
            900                 905                 910         


Ala Asp Asn Ala Ser Met Val Arg Val Asp Val Phe Thr Lys Asp Gly 
        915                 920                 925             


Lys Tyr Phe Leu Val Pro Ile Tyr Thr Trp Gln Val Ala Lys Gly Ile 
    930                 935                 940                 


Leu Pro Asn Lys Ala Val Ile Gln Gly Lys Asp Glu Glu Asp Trp Glu 
945                 950                 955                 960 


Asp Ile Asp Asp Ala Thr Phe Gln Phe Ser Leu His Pro Asn Asp Leu 
                965                 970                 975     


Ile Ser Val Lys Thr Lys Lys Asp Glu Phe Leu Gly Tyr Phe Asn Gly 
            980                 985                 990         


Leu Asn Arg His Thr Gly Gly Ile  Asn Ile Arg Thr His  Asp Leu Glu 
        995                 1000                 1005             


Lys Ser  Lys Gly Lys Gln Gly  Ile Phe Glu Gly Ile  Gly Val Lys 
    1010                 1015                 1020             


Ile Ala  Leu Ser Phe Glu Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1025                 1030                 1035             


Asn Ile  Arg Leu Cys Lys Pro  Ser Lys Arg Gln Pro  Val Arg 
    1040                 1045                 1050         


<210>  524
<211>  1052
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  524

Met Lys Asn Thr Ser Leu Asn Tyr Val Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ser Ser Val Gly Trp Ala Val Val Glu Ile Asp Glu Lys Glu Asn Pro 
            20                  25                  30          


Leu Ser Leu Ile Asp Val Gly Val Arg Thr Phe Glu Arg Ala Glu Thr 
        35                  40                  45              


Gln Lys Gly Glu Ser Leu Ala Leu Ala Arg Arg Leu Ala Arg Ser Ala 
    50                  55                  60                  


Arg Arg Leu Thr Gln Arg Arg Val Ala Arg Leu Lys Lys Ala Lys Arg 
65                  70                  75                  80  


Leu Leu Lys Ser Glu Asn Ile Leu Leu Ser Thr Asp Glu Cys Leu Pro 
                85                  90                  95      


His Gln Val Trp Gln Leu Arg Val Glu Gly Leu Glu Arg Lys Leu Glu 
            100                 105                 110         


Arg Gln Glu Trp Ala Ala Val Leu Leu His Leu Ile Lys His Arg Gly 
        115                 120                 125             


Tyr Leu Ser Gln Arg Lys Asn Glu Ser Lys Ser Glu Asn Lys Glu Leu 
    130                 135                 140                 


Gly Ala Leu Leu Ser Gly Val Ala Asn Asn His Lys Leu Leu Gln Gln 
145                 150                 155                 160 


Thr Thr Tyr Arg Thr Pro Ala Glu Leu Ala Val Lys Lys Phe Glu Val 
                165                 170                 175     


Glu Glu Gly His Ile Arg Asn Gln Gln Gly Ser Tyr Thr His Thr Phe 
            180                 185                 190         


Ser Arg Leu Asp Leu Leu Ser Glu Met Glu Leu Leu Phe Ser Arg Gln 
        195                 200                 205             


Gln His Phe Gly Asn Pro Phe Thr Ser Glu Lys Leu Leu Glu Asn Leu 
    210                 215                 220                 


Thr Ala Leu Leu Met Trp Gln Lys Pro Ala Leu Ser Gly Asp Ala Ile 
225                 230                 235                 240 


Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Asn Glu Tyr Lys Ala Ala 
                245                 250                 255     


Lys His Thr Tyr Ser Ala Glu Arg Phe Ile Trp Ile Thr Lys Leu Asn 
            260                 265                 270         


Asn Leu Arg Ile Gln Glu Asn Gly Leu Glu Arg Ala Leu Asn Asp Asn 
        275                 280                 285             


Glu Arg Leu Ala Leu Met Lys Gln Pro Tyr Glu Lys Ala Lys Leu Thr 
    290                 295                 300                 


Tyr Ala Gln Val Arg Ser Ile Leu Asn Leu Ser Asp Glu Ala Ile Phe 
305                 310                 315                 320 


Lys Gly Leu Arg Tyr Ser Gly Glu Asp Lys Lys Thr Ser Glu Thr Lys 
                325                 330                 335     


Thr Val Leu Met Glu Met Lys Ala Tyr His Gln Ile Arg Leu Ala Leu 
            340                 345                 350         


Glu Lys Asn Asp Leu Lys Thr Glu Trp Ala Glu Leu Lys Val Asn Ser 
        355                 360                 365             


Ser Leu Leu Asp Glu Ile Gly Thr Ala Phe Ser Leu Tyr Lys Thr Asp 
    370                 375                 380                 


Glu Asp Ile Ser Thr Cys Leu Ala Gly Lys Leu Ser Gln Pro Val Leu 
385                 390                 395                 400 


Asn Ala Leu Leu Glu Asn Leu Asn Phe Asp Lys Phe Ile Gln Leu Ser 
                405                 410                 415     


Leu Lys Ala Leu His Lys Ile Leu Pro Leu Met Gln Gln Gly Leu Arg 
            420                 425                 430         


Tyr Asp Glu Ala Cys Ala Glu Ile Tyr Gly Asp His Tyr Gly Lys Lys 
        435                 440                 445             


Thr Glu Glu Asn His Leu Leu Leu Pro Gln Ile Pro Ala Asp Glu Ile 
    450                 455                 460                 


Arg Asn Pro Val Val Leu Arg Thr Leu Thr Gln Ala Arg Lys Val Ile 
465                 470                 475                 480 


Asn Gly Val Val Arg Leu Tyr Ser Ser Pro Ala Arg Ile His Ile Glu 
                485                 490                 495     


Thr Gly Arg Glu Val Gly Lys Ser Tyr Gln Asp Arg Arg Glu Leu Glu 
            500                 505                 510         


Lys Arg Gln Glu Glu Asn Arg Lys Gln Arg Glu Lys Ala Ile Ser Glu 
        515                 520                 525             


Ile Lys Thr Leu Phe Pro Asn Phe Ser Gly Glu Pro Lys Ala Lys Asp 
    530                 535                 540                 


Ile Leu Lys Met Arg Leu Tyr Tyr Gln Gln Asn Ala Lys Cys Leu Tyr 
545                 550                 555                 560 


Ser Gly Lys Pro Leu Glu Leu His Arg Leu Leu Glu Gln Gly Tyr Val 
                565                 570                 575     


Glu Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe 
            580                 585                 590         


Asn Asn Lys Val Leu Val Leu Ala Asn Glu Asn Gln Asn Lys Gly Asn 
        595                 600                 605             


Leu Thr Pro Phe Glu Trp Leu Asp Gly Lys Asn Asn Ser Glu Arg Trp 
    610                 615                 620                 


Lys Ala Phe Lys Ala Leu Val Glu Thr Ser Ala Phe Pro Tyr Thr Lys 
625                 630                 635                 640 


Lys Gln Arg Ile Leu Ser Gln Lys Leu Asp Glu Lys Gly Phe Ile Glu 
                645                 650                 655     


Arg Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe Leu Cys Asn Phe 
            660                 665                 670         


Ile Ala Asp Asn Met His Leu Thr Gly Glu Gly Lys Arg Lys Val Phe 
        675                 680                 685             


Ala Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg Gly Arg Trp Gly Leu 
    690                 695                 700                 


Ala Lys Ser Arg Glu Asp Asn Asp Arg His His Ala Leu Asp Ala Val 
705                 710                 715                 720 


Val Val Ala Cys Ser Thr Val Ser Met Gln Gln Lys Ile Thr Arg Phe 
                725                 730                 735     


Val Arg Phe Glu Glu Gly Asn Val Phe Thr Gly Glu Arg Ile Asp Arg 
            740                 745                 750         


Glu Thr Gly Glu Ile Ile Pro Leu His Phe Pro Ala Pro Trp Lys Phe 
        755                 760                 765             


Phe Arg Glu Glu Val Glu Ile Arg Ile Phe Ser Asp Asn Pro Lys Leu 
    770                 775                 780                 


Glu Leu Glu Asn Arg Leu Ile Ala Arg Pro Gln Ala Asn His Glu Phe 
785                 790                 795                 800 


Val Gln Pro Leu Phe Val Ser Arg Met Pro Thr Arg Lys Met Thr Gly 
                805                 810                 815     


Gln Gly His Met Glu Thr Ile Lys Ser Ala Lys Arg Leu Asn Glu Gly 
            820                 825                 830         


Leu Ser Val Ile Lys Met Leu Leu Thr Lys Leu Arg Leu Lys Asp Leu 
        835                 840                 845             


Glu Ser Met Val Asn Arg Glu Arg Glu Lys Glu Leu Tyr Glu Ala Leu 
    850                 855                 860                 


Lys Ala Arg Leu Glu Ala Phe Asn Asp Glu Pro Ala Lys Ala Phe Ala 
865                 870                 875                 880 


Glu Pro Phe Met Lys Lys Gly Gly Ala Ile Val Lys Ser Val Arg Val 
                885                 890                 895     


Glu Gln Val Gln Lys Ser Gly Val Leu Val Arg Asp Gly Asn Gly Val 
            900                 905                 910         


Ala Asp Asn Ala Ser Met Val Arg Val Asp Val Phe Thr Lys Gly Gly 
        915                 920                 925             


Lys Tyr Phe Leu Val Pro Ile Tyr Thr Trp Gln Val Ala Lys Gly Ile 
    930                 935                 940                 


Leu Pro Asn Lys Ala Val Ile Gln Gly Lys Asn Glu Glu Asp Trp Glu 
945                 950                 955                 960 


Val Met Asp Asp Ala Thr Phe Arg Phe Ser Leu His Gln Asn Asp Leu 
                965                 970                 975     


Val Lys Leu Val Thr Lys Lys Lys Thr Ile Phe Gly Tyr Phe Asn Gly 
            980                 985                 990         


Leu Asn Arg Ala Thr Gly Asn Ile  Asp Ile Lys Glu His  Asp Leu Glu 
        995                 1000                 1005             


Lys Ser  Lys Val Lys Asp Gly  Ile His Gln Ser Ile  Gly Ile Lys 
    1010                 1015                 1020             


Leu Ala  Leu Ser Phe Glu Lys  Tyr Gln Val Asp Glu  Leu Gly Lys 
    1025                 1030                 1035             


Asn Ile  Arg Leu Cys Lys Ser  Ser Lys Arg Gln Ala  Val Arg 
    1040                 1045                 1050         


<210>  525
<211>  1052
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  525

Met Ile Gly Glu His Val Arg Gly Gly Cys Leu Phe Asp Asp His Trp 
1               5                   10                  15      


Thr Pro Asn Trp Gly Ala Phe Arg Leu Pro Asn Thr Val Arg Thr Phe 
            20                  25                  30          


Thr Lys Ala Glu Asn Pro Lys Asp Gly Ser Ser Leu Ala Glu Pro Arg 
        35                  40                  45              


Arg Gln Ala Arg Gly Leu Arg Arg Arg Leu Arg Arg Lys Thr Gln Arg 
    50                  55                  60                  


Leu Glu Asp Leu Arg Arg Leu Leu Ala Lys Glu Gly Val Leu Ser Leu 
65                  70                  75                  80  


Ser Asp Leu Glu Thr Leu Phe Arg Glu Thr Pro Ala Lys Asp Pro Tyr 
                85                  90                  95      


Gln Leu Arg Ala Glu Gly Leu Asp Arg Pro Leu Ser Phe Pro Glu Trp 
            100                 105                 110         


Val Arg Val Leu Tyr His Ile Thr Lys His Arg Gly Phe Gln Ser Asn 
        115                 120                 125             


Arg Arg Asn Pro Val Glu Asp Gly Gln Glu Arg Ser Arg Gln Glu Glu 
    130                 135                 140                 


Glu Gly Lys Leu Leu Ser Gly Val Gly Glu Asn Glu Arg Leu Leu Arg 
145                 150                 155                 160 


Glu Gly Gly Tyr Arg Thr Ala Gly Glu Met Leu Ala Arg Asp Pro Lys 
                165                 170                 175     


Phe Gln Asp His Arg Arg Asn Arg Ala Gly Asp Tyr Ser His Thr Leu 
            180                 185                 190         


Ser Arg Ser Leu Leu Leu Glu Glu Ala Arg Arg Leu Phe Gln Ser Gln 
        195                 200                 205             


Arg Thr Leu Gly Asn Pro His Ala Ser Ser Asn Leu Glu Glu Ala Phe 
    210                 215                 220                 


Leu His Leu Val Ala Phe Gln Asn Pro Phe Ala Ser Gly Glu Asp Ile 
225                 230                 235                 240 


Arg Asn Lys Ala Gly His Cys Ser Leu Glu Pro Asp Gln Ile Arg Ala 
                245                 250                 255     


Pro Arg Arg Ser Ala Ser Ala Glu Thr Phe Met Leu Leu Gln Lys Thr 
            260                 265                 270         


Gly Asn Leu Arg Leu Ile His Arg Arg Thr Gly Glu Glu Arg Pro Leu 
        275                 280                 285             


Thr Asp Lys Glu Arg Glu Gln Ile His Leu Leu Ala Trp Lys Gln Glu 
    290                 295                 300                 


Lys Val Thr His Lys Thr Leu Arg Arg His Leu Glu Ile Pro Glu Glu 
305                 310                 315                 320 


Trp Leu Phe Thr Gly Leu Pro Tyr His Arg Ser Gly Asp Lys Ala Glu 
                325                 330                 335     


Glu Lys Leu Phe Val His Leu Ala Gly Ile His Glu Ile Arg Lys Ala 
            340                 345                 350         


Leu Asp Lys Gly Pro Asp Pro Ala Val Trp Asp Thr Leu Arg Ser Arg 
        355                 360                 365             


Arg Asp Leu Leu Asp Ser Ile Ala Asp Thr Leu Thr Phe Tyr Lys Asn 
    370                 375                 380                 


Glu Asp Glu Ile Leu Pro Arg Leu Glu Ser Leu Gly Leu Ser Pro Glu 
385                 390                 395                 400 


Asn Ala Arg Ala Leu Ala Pro Leu Ser Phe Ser Gly Thr Ala His Leu 
                405                 410                 415     


Ser Leu Ser Ala Leu Gly Lys Leu Leu Pro His Leu Glu Glu Gly Lys 
            420                 425                 430         


Ser Tyr Thr Gln Ala Arg Ala Asp Ala Gly Tyr Ala Ala Pro Pro Pro 
        435                 440                 445             


Asp Arg His Pro Lys Leu Pro Pro Leu Glu Glu Ala Asp Trp Arg Asn 
    450                 455                 460                 


Pro Val Val Phe Arg Ala Leu Thr Gln Thr Arg Lys Val Val Asn Ala 
465                 470                 475                 480 


Leu Val Arg Arg Tyr Gly Pro Pro Trp Cys Ile His Leu Glu Thr Ala 
                485                 490                 495     


Arg Glu Leu Ser Gln Pro Ala Lys Val Arg Arg Arg Ile Glu Thr Glu 
            500                 505                 510         


Gln Gln Ala Asn Glu Lys Lys Lys Gln Gln Ala Glu Arg Glu Phe Leu 
        515                 520                 525             


Asp Ile Val Gly Thr Ala Pro Gly Pro Gly Asp Leu Leu Lys Met Arg 
    530                 535                 540                 


Leu Trp Arg Glu Gln Gly Gly Phe Cys Pro Tyr Cys Glu Glu Tyr Leu 
545                 550                 555                 560 


Asn Pro Thr Arg Leu Ala Glu Pro Gly Tyr Ala Glu Met Asp His Ile 
                565                 570                 575     


Leu Pro Tyr Ser Arg Ser Leu Asp Asn Gly Trp His Asn Arg Val Leu 
            580                 585                 590         


Val His Gly Lys Asp Asn Arg Asp Lys Gly Asn Arg Thr Pro Phe Glu 
        595                 600                 605             


Ala Phe Gly Gly Asp Thr Ala Arg Trp Asp Arg Leu Val Ala Trp Val 
    610                 615                 620                 


Gln Ala Ser His Leu Ser Ala Pro Lys Lys Arg Asn Leu Leu Arg Glu 
625                 630                 635                 640 


Asp Phe Gly Glu Glu Ala Glu Arg Glu Leu Lys Asp Arg Asn Leu Thr 
                645                 650                 655     


Asp Thr Arg Phe Ile Thr Lys Thr Ala Ala Thr Leu Leu Arg Asp Arg 
            660                 665                 670         


Leu Thr Phe His Pro Glu Ala Pro Lys Asp Pro Val Met Thr Leu Asn 
        675                 680                 685             


Gly Arg Leu Thr Ala Phe Leu Arg Lys Gln Trp Gly Leu His Lys Asn 
    690                 695                 700                 


Arg Lys Asn Gly Asp Leu His His Ala Leu Asp Ala Ala Val Leu Ala 
705                 710                 715                 720 


Val Ala Ser Arg Ser Phe Val Tyr Arg Leu Ser Ser His Asn Ala Ala 
                725                 730                 735     


Trp Gly Glu Leu Pro Arg Gly Arg Glu Ala Glu Asn Gly Phe Ser Leu 
            740                 745                 750         


Pro Tyr Pro Ala Phe Arg Ser Glu Val Leu Ala Arg Leu Cys Pro Thr 
        755                 760                 765             


Arg Glu Glu Ile Leu Leu Arg Leu Asp Gln Gly Gly Val Gly Tyr Asp 
    770                 775                 780                 


Glu Ala Phe Arg Asn Gly Leu Arg Pro Val Phe Val Ser Arg Ala Pro 
785                 790                 795                 800 


Ser Arg Arg Leu Arg Gly Lys Ala His Met Glu Thr Leu Arg Ser Pro 
                805                 810                 815     


Lys Trp Lys Asp His Pro Glu Gly Pro Arg Thr Ala Ser Arg Ile Pro 
            820                 825                 830         


Leu Lys Asp Leu Asn Leu Glu Lys Leu Glu Arg Met Val Gly Lys Asp 
        835                 840                 845             


Arg Asp Arg Lys Leu Tyr Glu Ala Leu Arg Glu Arg Leu Ala Ala Phe 
    850                 855                 860                 


Gly Gly Asn Gly Lys Lys Ala Phe Val Ala Pro Phe Arg Lys Pro Cys 
865                 870                 875                 880 


Arg Ser Gly Glu Gly Pro Leu Val Arg Ser Leu Arg Ile Phe Asp Ser 
                885                 890                 895     


Gly Tyr Ser Gly Val Glu Leu Arg Asp Gly Gly Glu Val Tyr Ala Val 
            900                 905                 910         


Ala Asp His Glu Ser Met Val Arg Val Asp Val Tyr Ala Lys Lys Asn 
        915                 920                 925             


Arg Phe Tyr Leu Val Pro Val Tyr Val Ala Asp Val Ala Arg Gly Ile 
    930                 935                 940                 


Val Lys Asn Arg Ala Ile Val Ala His Lys Ser Glu Glu Glu Trp Asp 
945                 950                 955                 960 


Leu Val Asp Gly Ser Phe Asp Phe Arg Phe Ser Leu Phe Pro Gly Asp 
                965                 970                 975     


Leu Val Glu Ile Glu Lys Lys Asp Gly Ala Tyr Leu Gly Tyr Tyr Lys 
            980                 985                 990         


Ser Cys His Arg Gly Asp Gly Arg  Leu Leu Leu Asp Arg  His Asp Arg 
        995                 1000                 1005             


Met Pro  Arg Glu Ser Asp Cys  Gly Thr Phe Tyr Val  Ser Thr Arg 
    1010                 1015                 1020             


Lys Asp  Val Leu Ser Met Ser  Lys Tyr Gln Val Asp  Pro Leu Gly 
    1025                 1030                 1035             


Glu Ile  Arg Leu Val Gly Ser  Glu Lys Pro Pro Phe  Val Leu 
    1040                 1045                 1050         


<210>  526
<211>  1053
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  526

Met Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
                405                 410                 415     


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Ile Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Pro Pro Arg  Ile Ile Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Val Lys Ser Lys Lys  His Pro Gln Ile Ile  Lys Lys Gly 
    1040                 1045                 1050             


<210>  527
<211>  1053
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  527

Met Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asn Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Thr Arg Arg Leu Lys Arg Arg Arg Arg His Arg Leu 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp His Asn Phe Leu Thr Asp Asn 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Ser Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Asn Gln Lys Ile Ser Glu Glu Glu Phe Ser Val Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Val 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ala Val Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Gln Gln Ile Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Phe Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly Gln Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Ala Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Phe Lys Val Tyr His Asp Ile Lys Ser Ile Thr 
                325                 330                 335     


Ser Arg Lys Glu Ile Leu Glu Asn Ala Glu Leu Leu Asn Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Gly Glu Leu 
        355                 360                 365             


Ala Arg Leu Tyr Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Lys Leu Ala Gly Tyr Thr Gly Thr His Arg Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Ile Asp Glu Leu Trp His Thr Ser Asp Asn Gln Met Glu 
                405                 410                 415     


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Phe Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Phe Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Phe 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Ile Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Lys Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Tyr 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Glu Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asp Leu Asp Ile Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Lys Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Thr Asn Lys Val Met Glu Asp Gln Ile Val Glu Glu Gln 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Gln Asp Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Val 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Lys Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Val Ile Met Glu Gln Tyr Ser 
        835                 840                 845             


Asn Glu Lys Asn Pro Leu Tyr Lys Tyr His Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Val Tyr Leu Asp Ile Thr Arg Asp 
                885                 890                 895     


Tyr Leu Lys Ser Arg Asn Lys Ile Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Ile Ile Lys Lys Glu Asp Tyr Tyr Glu Val Asp Ser 
    930                 935                 940                 


Asp Ser Tyr Glu Glu Ala Lys Ile Val Lys Lys Val Ser Glu Gln Ala 
945                 950                 955                 960 


Gln Phe Val Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Val Asp Gly 
                965                 970                 975     


Glu Leu Phe Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Val Asp Ile Thr  Tyr Lys Glu Tyr Leu  Glu Asn Ile 
        995                 1000                 1005             


Asn Asp  Lys Lys Ser Ser Arg  Ile Ile Lys Thr Ile  Ser Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Ala Lys Ser Lys Gln  Arg Pro Gln Met Thr  Met Lys Gly 
    1040                 1045                 1050             


<210>  528
<211>  1053
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  528

Met Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Gly Arg Leu Lys Arg Arg Arg Arg His Arg Leu 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Thr 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Glu Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Ala Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Glu Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Ser Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Glu Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Val Lys Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Met Ala 
                405                 410                 415     


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Glu Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Thr Val Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Ser Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Gln His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr His Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Met Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asn Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Lys Ala Lys Lys Leu Lys Lys Ile Ser Asp Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asp Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Thr Asp Leu Ile Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Val Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Ser Pro Arg  Ile Phe Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Thr Leu 
    1025                 1030                 1035             


Tyr Glu  Val Asn Ser Lys Lys  His Pro Gln Met Ile  Met Lys Gly 
    1040                 1045                 1050             


<210>  529
<211>  1053
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  529

Met Ser Gly Met Val Phe Gly Ile Asp Leu Gly Ile Ala Ser Cys Gly 
1               5                   10                  15      


Trp Ala Val Leu Arg Gln Pro Gln Arg Asp Gly Asp Pro Gly Glu Ile 
            20                  25                  30          


Val Asp Leu Gly Ser Trp Met Phe Asp Val Pro Glu Thr Asp Lys Glu 
        35                  40                  45              


Arg Thr Pro Thr Asn Gln Val Arg Arg Gly Asn Arg Leu Leu Arg Arg 
    50                  55                  60                  


Val Ile Arg Arg Arg Ala Gln Arg Met Val Glu Ile Arg Arg Leu Phe 
65                  70                  75                  80  


His Asp His Gly Leu Leu Ala Gly His Ala Pro Glu Ala Leu Lys Arg 
                85                  90                  95      


Ala Gly Leu Asp Pro Trp Asp Leu Arg Ala Arg Ser Leu Asp Lys Val 
            100                 105                 110         


Leu Glu Pro Ala Ala Leu Ala Val Ala Leu Gly His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Phe Lys Ser Ala Ala Arg Arg Lys Glu Ala Asn Thr Ala Gly 
    130                 135                 140                 


Asp Asp Gln Lys Met Leu Lys Ala Leu Glu Ala Thr His Glu Arg Leu 
145                 150                 155                 160 


Gly Arg Tyr Arg Thr Ile Gly Glu Met Phe Ala Arg Asp Pro Asp Phe 
                165                 170                 175     


Glu Ser Arg Lys Arg Asn Arg Asp Gly Met Phe Asp Arg Thr Gln Gly 
            180                 185                 190         


Arg Asp Asp Leu Leu His Glu Val Gly Glu Ile Phe Lys Ala Gln Arg 
        195                 200                 205             


Arg Leu Gly Ser Ala Leu Ala Thr Ala Glu Leu Glu Gln Ala Phe Thr 
    210                 215                 220                 


Ala Ile Ala Phe Arg Gln Leu Pro Ile Gln Asp Ser Glu Arg Leu Val 
225                 230                 235                 240 


Gly Leu Cys Gln Phe Glu Pro Lys Glu Lys Arg Ala Ala Arg Phe Ala 
                245                 250                 255     


Pro Ser Phe Glu Arg Phe Arg Leu Leu Gln Arg Leu Thr Asn Leu Arg 
            260                 265                 270         


Val Val Thr Val Glu Gly Glu Arg Pro Leu Thr Ala Asp Glu Ile Ala 
        275                 280                 285             


Ala Ala Ala Ala Asp Leu Gly Arg Thr Ala Lys Leu Ser Val Lys Glu 
    290                 295                 300                 


Val Arg Lys Arg Ile Gly Leu Ala Ala Asp His Arg Phe Ala Ala Phe 
305                 310                 315                 320 


Lys Ala Asp Glu Glu Asp Arg Asp Ile Ile Ala Arg Thr Gly Glu Ala 
                325                 330                 335     


Leu His Gly Thr Tyr Arg Leu Arg Lys Ala Leu Gly Glu Gly Leu Trp 
            340                 345                 350         


Ala Glu Met Leu Pro Gly Gln Leu Asp Ala Ile Ala His Ala Leu Ser 
        355                 360                 365             


Phe Phe Glu Thr Gln Asp Val Ile Leu Lys Glu Leu Asp Lys Leu Asp 
    370                 375                 380                 


Leu Pro Ala Gly Val Arg Asp Ala Ile Ala Thr Gly Leu Asp Ala Gly 
385                 390                 395                 400 


Ala Phe Ala Arg Phe Lys Gly Thr Gly His Ile Ser Ala Arg Ala Ala 
                405                 410                 415     


Arg Ala Leu Leu Pro His Leu Glu Ala Gly Leu Arg Tyr Asp Gln Ala 
            420                 425                 430         


Cys Thr Lys Ala Gly Tyr Asp His Ala Ala Ser Arg Trp Ala Lys Arg 
        435                 440                 445             


Glu Gln Val Ala Asp Lys Ala Ala Phe Asn Arg Leu Val Thr Asp Met 
    450                 455                 460                 


Gly Ala Glu Ile Ala Asn Pro Ile Ala Arg Lys Ser Leu Thr Glu Ala 
465                 470                 475                 480 


Leu Lys Gln Leu Trp Ala Met Arg Asn Arg Trp Gly Leu Pro Asp Ala 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Val Gly Asn Ser Leu Glu Lys Arg 
            500                 505                 510         


Arg Glu Ile Glu Arg Ala Ile Glu Lys Asn Thr Ala Ala Arg Glu Arg 
        515                 520                 525             


Glu Arg Gly Glu Ala Arg Asp Leu Leu Gly Ile Asp Asp Val Ser Gly 
    530                 535                 540                 


Asp Thr Leu Leu Arg Tyr Arg Leu Trp Lys Glu Gln Ala Gly Arg Cys 
545                 550                 555                 560 


Pro Tyr Thr Asp Ala Pro Ile Pro Pro Gly Ala Ile Ile Ala Thr Asp 
                565                 570                 575     


Asn Ser Phe Gln Val Asp His Ile Leu Pro Trp Ser Arg Phe Gly Asp 
            580                 585                 590         


Asp Ser Phe Ala Asn Lys Val Leu Cys Ala Thr Ala Ala Asn Gln Arg 
        595                 600                 605             


Lys Lys Gly Gln Thr Pro Cys Glu Trp Ile Thr Ala Ala Gln Gly Glu 
    610                 615                 620                 


Glu Gly Trp Ala Thr Phe Val Ala Arg Ile Glu Gly Asn Ala Ala Phe 
625                 630                 635                 640 


Arg Gly Pro Lys Lys Arg Asn Tyr Val Leu Lys Asn Ala Lys Glu Ala 
                645                 650                 655     


Glu Glu Arg Phe Arg Ala Arg Asn Leu Asn Asp Thr Arg Tyr Ala Ala 
            660                 665                 670         


Arg Leu Leu Ala Glu Ala Val Lys Leu Phe Tyr Pro Glu Gly Glu Arg 
        675                 680                 685             


Gln Asp Lys Gly Gly Val Arg Arg Val Phe Thr Arg Pro Gly Gly Leu 
    690                 695                 700                 


Thr Ala Ala Leu Arg His Ala Trp Gly Val Glu Ala Leu Lys Lys Arg 
705                 710                 715                 720 


Asp Gly Lys Arg Val Asp Asp Ala Arg His His Ala Leu Asp Ala Leu 
                725                 730                 735     


Val Val Ala Ala Ile Gly Glu Gly Glu Val Gln Arg Leu Thr Arg Ser 
            740                 745                 750         


Tyr Gln Glu Trp Glu Gln Gln Gly Leu Ala Arg Pro Leu Arg Arg Val 
        755                 760                 765             


Asp Pro Pro Trp Gly Asp Phe His Ser Phe Arg Arg Glu Val Lys Asp 
    770                 775                 780                 


Ala Tyr Asp Gly Ile Phe Val Ala Arg Pro Glu Arg Arg Arg Ala Arg 
785                 790                 795                 800 


Gly Glu Gly His Ala Ala Thr Ile Arg Gln Val Arg Glu Arg Asp Gly 
                805                 810                 815     


Ala Ala Val Val Phe Glu Arg Lys Ala Ile Ala Asp Leu Ser Glu Lys 
            820                 825                 830         


Arg Leu Ala Asp Ile Lys Asp Pro Glu Arg Asn Gln Ala Ile Val Glu 
        835                 840                 845             


Ala Ile Arg Gln Trp Ile Val Asp Gly Arg Pro Ala Asp Arg Leu Pro 
    850                 855                 860                 


Arg Ser Pro Ala Gly Asp Glu Ile Arg Lys Val Arg Leu Arg Thr Lys 
865                 870                 875                 880 


Gly Lys Pro Ala Val Gln Val Arg Gly Gly Ala Ala Asp Arg Gly Glu 
                885                 890                 895     


Ile Val Arg Val Asp Val Phe Thr Lys Pro Asn Lys Lys Gly Lys Asn 
            900                 905                 910         


Glu Phe Tyr Leu Val Pro Ile Tyr Pro His Gln Val Met Asn Lys Ala 
        915                 920                 925             


Glu Trp Pro Thr Pro Pro Met Arg Ala Val Val Ala Tyr Lys Asp Glu 
    930                 935                 940                 


Ser Glu Trp Thr Leu Leu Asp Glu Asn Phe Gly Phe Leu Phe Ser Leu 
945                 950                 955                 960 


Phe Pro Arg Ser Tyr Val Glu Val Thr Lys Pro Gly Gly Glu Val Leu 
                965                 970                 975     


Ser Gly Tyr Phe Gln Gly Met Asp Arg Ser Thr Gly Ala Ile Ser Leu 
            980                 985                 990         


Phe Asn His Arg Asp Ser Arg Ser  Leu Thr Asp Asp Ser  Gly Asn Ser 
        995                 1000                 1005             


Thr Arg  Gly Ile Gly Ala Lys  Thr Leu Leu Thr Met  Lys Lys Tyr 
    1010                 1015                 1020             


Ser Val  Asp Arg Phe Gly Lys  Arg Ala Glu Val Lys  Ser Glu Val 
    1025                 1030                 1035             


Arg Thr  Trp His Gly Val Ala  Cys Thr Tyr Pro Thr  Pro Pro Gly 
    1040                 1045                 1050             


<210>  530
<211>  1054
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  530

Met Lys Leu Thr Pro Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ala Val Val Glu Ile Asp Glu Gln Glu Asn Pro 
            20                  25                  30          


Leu Gly Leu Ile Asp Val Gly Val Arg Thr Phe Asp Arg Ala Glu Val 
        35                  40                  45              


Ala Lys Thr Gly Glu Ser Leu Ala Leu Ala Arg Arg Leu Ala Arg Ser 
    50                  55                  60                  


Ser Arg Arg Leu Val Lys Arg Arg Ala Asp Arg Ile Lys Lys Val Lys 
65                  70                  75                  80  


Arg Leu Leu Lys Ser Glu Asn Ile Leu Leu Ser Ala Asp Glu Gln Leu 
                85                  90                  95      


Pro Asn Asp Val Trp Gln Leu Arg Val Lys Gly Leu Asp Gln Lys Leu 
            100                 105                 110         


Glu Arg Gln Glu Trp Ala Ala Val Leu Leu His Leu Leu Lys His Arg 
        115                 120                 125             


Gly Tyr Leu Ser Gln Arg Lys Asn Glu Ser Lys Ser Glu Asn Lys Glu 
    130                 135                 140                 


Leu Gly Ala Leu Arg Ser Gly Val Ala Ala Asn His Gln Ile Leu Gln 
145                 150                 155                 160 


Ser Ala Glu Tyr Arg Thr Pro Ala Glu Ile Ala Val Lys Lys Phe His 
                165                 170                 175     


Val Glu Glu Gly His Ile Arg Asn Gln Arg Gly Ala Tyr Thr His Thr 
            180                 185                 190         


Phe Ser Arg Leu Asp Leu Leu Ala Glu Ile Glu Leu Leu Phe Thr Arg 
        195                 200                 205             


Gln Thr Glu Leu Gly Asn Pro His Thr Ser Ala Lys Leu Leu Glu Asn 
    210                 215                 220                 


Leu Thr Ala Leu Leu Met Trp Gln Lys Pro Ala Leu Ala Gly Glu Ala 
225                 230                 235                 240 


Ile Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Pro Thr Glu Tyr Lys 
                245                 250                 255     


Ala Ala Lys Asn Ser Tyr Ser Ala Glu Arg Phe Val Trp Leu Thr Lys 
            260                 265                 270         


Leu Asn Asn Leu Arg Ile Leu Glu Gln Gly Ala Glu Arg Ala Leu Thr 
        275                 280                 285             


Asp Asn Glu Arg Phe Ala Leu Leu Asp Gln Pro Tyr Glu Lys Ala Lys 
    290                 295                 300                 


Phe Thr Tyr Ala Gln Ala Arg Thr Met Leu Ala Leu Pro Asp Glu Ala 
305                 310                 315                 320 


Ile Phe Lys Gly Val Arg Tyr Gln Gly Glu Asp Lys Lys Ala Val Glu 
                325                 330                 335     


Thr Lys Thr Ile Leu Met Glu Met Lys Ala Tyr His Gln Ile Arg Lys 
            340                 345                 350         


Ala Leu Glu Asn Ala Asp Leu Lys Ala Glu Trp Asn Glu Leu Lys Asn 
        355                 360                 365             


Asn Ser Glu Leu Leu Asp Asp Ile Gly Thr Ala Phe Ser Leu His Lys 
    370                 375                 380                 


Thr Asp Glu Asp Ile Cys Arg Tyr Leu Asp Gly Lys Leu Ser Glu Ser 
385                 390                 395                 400 


Ile Leu Asn Ala Leu Leu Glu Asn Leu Asn Phe Asp Lys Phe Ile Gln 
                405                 410                 415     


Leu Ser Leu Lys Ala Leu Gln Gln Ile Leu Pro Leu Met Leu Gln Gly 
            420                 425                 430         


Gln Arg Tyr Asp Glu Ala Val Ser Ala Ile Tyr Gly Asp His Tyr Gly 
        435                 440                 445             


Lys Lys Ser Ala Glu Ile Asn Arg Leu Leu Pro Thr Ile Pro Ala Asp 
    450                 455                 460                 


Glu Ile Arg Asn Pro Val Val Leu Arg Thr Leu Thr Gln Ala Arg Lys 
465                 470                 475                 480 


Val Ile Asn Ala Val Val Arg Leu Tyr Gly Ser Pro Ala Arg Ile His 
                485                 490                 495     


Ile Glu Thr Gly Arg Glu Val Gly Lys Ser Tyr Gln Asp Arg Lys Lys 
            500                 505                 510         


Leu Glu Lys Gln Gln Glu Asp Asn Arg Lys His Arg Glu Ser Ala Val 
        515                 520                 525             


Gln Lys Phe Lys Glu Tyr Phe Pro Asn Phe Val Gly Glu Pro Lys Gly 
    530                 535                 540                 


Lys Asp Ile Leu Lys Met Arg Leu Tyr Glu Leu Gln Gln Ala Lys Cys 
545                 550                 555                 560 


Leu Tyr Ser Gly Lys Ser Leu Glu Leu His Arg Leu Leu Glu Lys Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp 
            580                 585                 590         


Ser Phe Asn Asn Lys Val Leu Val Leu Ala Asn Glu Asn Gln Asn Lys 
        595                 600                 605             


Gly Asn Leu Thr Pro Tyr Glu Trp Leu Asp Gly Lys Asn Asn Ser Glu 
    610                 615                 620                 


Pro Trp Gln Asn Phe Val Ala Arg Val Gln Thr Ser Gly Phe Ser His 
625                 630                 635                 640 


Thr Lys Lys Gln Arg Ile Leu Ser His Lys Leu Asp Glu Lys Gly Phe 
                645                 650                 655     


Ile Glu Arg Asn Leu Asn Asp Thr Arg Tyr Val Val Arg Phe Leu Cys 
            660                 665                 670         


Asn Phe Ile Ala Asp Asn Met Leu Leu Thr Gly Lys Gly Lys Arg Lys 
        675                 680                 685             


Val Phe Ala Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg Gly Arg Trp 
    690                 695                 700                 


Gly Leu Gln Lys Val Arg Asp Asp Asn Asp Arg His His Ala Leu Asp 
705                 710                 715                 720 


Ala Val Val Val Ala Cys Ser Thr Val Ala Met Gln Gln Lys Ile Thr 
                725                 730                 735     


Arg Phe Val Arg Tyr Glu Glu Gly Asn Val Phe Ser Gly Glu Arg Ile 
            740                 745                 750         


Asp Arg Glu Thr Gly Glu Ile Ile Pro Leu His Phe Pro Ser Pro Trp 
        755                 760                 765             


Ala Phe Phe Arg Glu Asn Val Glu Ile Arg Ile Phe Ser Glu Asn Pro 
    770                 775                 780                 


Lys Leu Glu Leu Glu Asn Arg Leu Pro Asp Tyr Pro Gln Tyr Asn His 
785                 790                 795                 800 


Glu Phe Val Gln Pro Leu Phe Val Ser Arg Met Pro Thr Arg Lys Met 
                805                 810                 815     


Thr Gly Gln Gly His Met Glu Thr Val Lys Ser Ala Lys Arg Leu Asp 
            820                 825                 830         


Glu Gly Leu Ser Val Leu Lys Val Pro Leu Thr Gln Leu Lys Leu Asn 
        835                 840                 845             


Asp Leu Glu Arg Met Val Asn Arg Glu Arg Glu Met Ala Leu Tyr Glu 
    850                 855                 860                 


Ser Leu Lys Val Arg Leu Glu Gln Phe Asp Asn Asp Pro Ala Lys Ala 
865                 870                 875                 880 


Phe Ala Glu Pro Phe Tyr Lys Lys Gly Gly Ala Leu Val Lys Ala Val 
                885                 890                 895     


Arg Val Glu Gln Thr Gln Lys Ser Gly Val Leu Val Arg Asp Gly Asn 
            900                 905                 910         


Gly Val Ala Asp Asn Ala Ser Met Val Arg Val Asp Val Phe Thr Lys 
        915                 920                 925             


Ala Gly Lys Tyr Phe Leu Val Pro Ile Tyr Thr Trp Gln Val Ala Lys 
    930                 935                 940                 


Gly Ile Leu Pro Asn Lys Ala Val Thr Ala Asn Val Asp Glu Ile Asp 
945                 950                 955                 960 


Trp Leu Glu Met Asp Glu Ser Tyr Gln Phe Ile Phe Thr Met Tyr Pro 
                965                 970                 975     


Asn Asp Leu Val Lys Val Lys Leu Lys Lys Glu Glu Phe Phe Gly Tyr 
            980                 985                 990         


Tyr Gly Gly Leu Asp Arg Ala Thr  Gly Ala Ile Val Ile  Lys Glu His 
        995                 1000                 1005             


Asp Leu  Glu Lys Ser Lys Gly  Arg Gln Gly Ile Tyr  Arg Ile Gly 
    1010                 1015                 1020             


Val Lys  Leu Ala Leu Ser Phe  Glu Lys Tyr Gln Val  Asp Glu Leu 
    1025                 1030                 1035             


Gly Lys  Asn Ile Arg Pro Cys  Arg Pro Thr Lys Arg  Gln His Val 
    1040                 1045                 1050             


Arg 
    


<210>  531
<211>  1054
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  531

Met Arg Ile Leu Gly Val Asp Leu Gly Val Ala Ser Val Gly Trp Ala 
1               5                   10                  15      


Leu Val Asn Asp Lys Asn Asn Lys Ile Ile Asp Ser Gly Val Arg Ile 
            20                  25                  30          


Phe Thr Gln Ser Thr Asn Arg Glu Gly Lys Thr Leu Ala Ala Val Arg 
        35                  40                  45              


Arg Ser Phe Arg Gly Gly Arg Arg Val Leu Ser Arg Arg Lys Gln Arg 
    50                  55                  60                  


Leu Asn Gln Leu Lys Lys Leu Phe Ile Ala His Lys Leu Leu Ser Lys 
65                  70                  75                  80  


Ser Glu Phe Lys His Leu Phe Asn Ser Lys Asn Arg Leu Asp Ile Trp 
                85                  90                  95      


Gln Leu Arg Ala Lys Gly Leu Glu Gln Gln Leu Asn Asn Lys Glu Trp 
            100                 105                 110         


Ala Arg Val Leu Tyr His Ile Ala Lys Arg Arg Ala Tyr Gln Ser Asn 
        115                 120                 125             


Arg Lys Ser Glu Glu Ser Gly Asn Thr Asp Lys Lys Lys Val Leu Ser 
    130                 135                 140                 


Gly Ile Ser Lys Asn Glu Lys Lys Met Asn Glu Leu Gly Tyr Lys Thr 
145                 150                 155                 160 


Ile Gly Glu Met Leu Tyr Thr Glu Asn Arg Tyr Pro Asp Asn Arg Ile 
                165                 170                 175     


Arg Arg Asn Lys Lys Asp Gln Tyr Lys His Ser Ile Ser Arg Asp Leu 
            180                 185                 190         


Leu Arg Lys Glu Ile Asp Val Leu Phe Glu Ser Gln Arg Asp Phe Gly 
        195                 200                 205             


Asn Gln Phe Ala Thr Asp Gly Leu Lys Glu His Tyr Lys Asp Ile Ala 
    210                 215                 220                 


Phe Thr Gln Arg Pro Ile Lys Phe Lys Ala Asp Leu Val Gly Lys Cys 
225                 230                 235                 240 


Thr Phe Glu Thr Gln Glu Tyr Arg Gly Ala Lys Asn Cys Phe Ser Val 
                245                 250                 255     


Glu Lys Ser Thr Leu Leu Ser Lys Ile Asn Asn Thr Asp Leu Ile Asp 
            260                 265                 270         


Lys Ser Thr Gly Glu Ile Lys Arg Leu Phe Glu Ile Ser Glu Phe Asp 
        275                 280                 285             


Lys Leu Leu Asp Ile Phe Tyr Ser Ile Lys Glu Val Lys Tyr Thr Thr 
    290                 295                 300                 


Leu Arg Lys Gln Leu Lys Ile Thr Asp Ala Phe Lys Phe Lys Gln Leu 
305                 310                 315                 320 


Asp Tyr His Gln Asn Phe Glu Tyr Lys Thr Asn Ile Lys Ala Lys Ile 
                325                 330                 335     


Glu Asp Phe Tyr Lys Leu Glu Asp Trp Leu Thr Pro Glu Gln Lys Val 
            340                 345                 350         


Leu Leu Glu Lys Gln Val Leu Asp Asp Lys Lys Glu Lys Phe Lys Cys 
        355                 360                 365             


Tyr Lys Tyr Ser Ser Ile Arg Ser Ile Leu Ser Leu Ser Glu Ser Gln 
    370                 375                 380                 


Lys Phe Asn Gly Val Lys Tyr Asp Thr Lys Val Glu Asn Thr Val Phe 
385                 390                 395                 400 


Gly Lys Leu Asn Gly Tyr His Ser Ile Lys Lys Glu Leu Asn Lys Glu 
                405                 410                 415     


Val Phe Asp Leu Leu Phe Ile Asp Lys Asp Lys Phe Asn Lys Ile Ala 
            420                 425                 430         


Glu Ile Leu Ser Tyr Gln Lys Asp Asp Lys Ser Ala Thr Gln Asp Leu 
        435                 440                 445             


Lys Lys Gln Val Phe Asn Asp Phe Asn Met Asp Lys Thr Ile Val Asn 
    450                 455                 460                 


Glu Ala Ile Asn Ser Leu Leu Asn Ile Ser Phe Ser Gly Phe Asn Ser 
465                 470                 475                 480 


Leu Ser Leu Lys Ala Ile Asn Lys Leu Leu Pro Tyr Leu Cys Gln Gly 
                485                 490                 495     


Leu Lys Tyr His Asp Ala Val Ala His Ser Glu Tyr Lys His His Ser 
            500                 505                 510         


Gln Phe Lys Ala Ala Asn Pro Gln Gln Tyr Leu Arg Pro Leu Asn Lys 
        515                 520                 525             


Glu Glu Asn Tyr Gln Ile Thr Ser Pro Thr Val Lys Arg Val Phe Ser 
    530                 535                 540                 


Gln Phe Arg Lys Val Leu Asn Ala Val Ile Arg Lys His Gly Ser Phe 
545                 550                 555                 560 


Asp Ala Met His Ile Glu Met Ala Arg Glu Leu Lys Asn Ser Lys Lys 
                565                 570                 575     


Ile Lys Phe Glu Ile Gln Thr Gly Gln Lys Glu Tyr Ala Gln Glu Lys 
            580                 585                 590         


Glu Ala Ile Arg Val Lys Phe Leu Glu Ile Phe Lys Tyr Glu Gly Ser 
        595                 600                 605             


Gly Arg Asp Leu Leu Lys Leu Arg Leu Tyr Glu Gln Gln His Gly Lys 
    610                 615                 620                 


Cys Ile Tyr Ser Lys Lys Glu Ile Glu Ile Asn Lys Leu Leu Glu Asp 
625                 630                 635                 640 


Gly Tyr Val Glu Ile Asp His Ile Leu Pro Trp Ser Arg Thr Phe Asp 
                645                 650                 655     


Asn Ser Leu Asn Asn Lys Ala Leu Cys Leu Ser Ala Glu Asn Gln Asn 
            660                 665                 670         


Lys Ala Asn Gln Thr Pro Phe Glu Tyr Leu Thr Asn Ser Asp Lys Asn 
        675                 680                 685             


Ser Lys Asp Ser Lys Glu Trp Gln Glu Tyr Lys Asn Tyr Val Ser Ser 
    690                 695                 700                 


Phe Ala Gly Ile Lys Gln Ala Lys Lys His Lys Leu Leu Asn Thr Thr 
705                 710                 715                 720 


Leu Pro Pro Arg Arg Gly Asn Asp Phe Ser Asp Asp Ile Lys Asn Pro 
                725                 730                 735     


Glu Ser Gly Phe Leu Ala Arg Asn Leu Asn Asp Thr Ala Tyr Ala Ser 
            740                 745                 750         


Arg Phe Ile Lys Asn Phe Val Gln Asn His Leu Gln Phe Ser Gln Asn 
        755                 760                 765             


Asp Thr Ile Lys Gln Lys Val Lys Val Arg Ser Gly Ala Leu Thr Asn 
    770                 775                 780                 


Gln Leu Arg Tyr Asn Trp Gly Ile Glu Glu Lys Asn Arg Asp Asn Asn 
785                 790                 795                 800 


Leu His His Ala Glu Asp Ala Ile Ile Leu Ala Phe Ala Thr Gln Gly 
                805                 810                 815     


Glu Val Gln Arg Met Ser Thr Ile Ser Ala Lys Arg Glu Asp Phe Lys 
            820                 825                 830         


Tyr Gln Thr Ala Lys Glu Arg Lys Ile Lys Phe Thr Pro Pro Phe Ser 
        835                 840                 845             


Asp Phe Lys Ser Val Leu Asp Glu Ser Val Gly Asp Ile Phe Val Ser 
    850                 855                 860                 


Phe Ala Pro Arg Arg Lys Ile Ser Gly Ala Ala His Lys Ala Thr Ile 
865                 870                 875                 880 


Lys Pro Lys Asp Thr Gly Gly Lys Tyr Lys Phe Pro Val Asn Asn Gly 
                885                 890                 895     


Met Ala Glu Asn Gly Val Ile Gln Arg Val Asp Val Phe Lys Asn Ala 
            900                 905                 910         


Lys Asn Lys Tyr Gln Phe Ile Val Phe Tyr Pro Ala Asp Phe Tyr Lys 
        915                 920                 925             


Asp Asp Phe Pro Glu Leu Asp Leu Lys Gly Asn Glu Ile Asp Lys Asn 
    930                 935                 940                 


Val Gln Phe Leu Phe Ser Leu Phe Lys Asp Asp Leu Ile Glu Phe Lys 
945                 950                 955                 960 


Thr Lys Lys Thr Lys Thr Lys Asp Ser Lys His Leu Leu Ala Tyr Phe 
                965                 970                 975     


Lys Tyr Ile Arg Ser Asp Gly Lys Ile Ala Phe Gln Gln Asn Tyr Lys 
            980                 985                 990         


Ala Glu Ile Glu Arg Lys Glu Ser  Asp Asn Lys Lys Gly  Tyr Lys Asp 
        995                 1000                 1005             


Ile Leu  Leu Ala Thr Gly Ser  Gly Leu Ala Ser Leu  Lys Lys Tyr 
    1010                 1015                 1020             


Gln Val  Ser Val Leu Gly Asp  Ile Ala Glu Val Lys  Asn Glu Lys 
    1025                 1030                 1035             


Arg Leu  Pro Leu Ile Lys Gln  Met Tyr Lys Ser Lys  Pro Lys Lys 
    1040                 1045                 1050             


Lys 
    


<210>  532
<211>  1054
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  532

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Lys Leu Leu Glu Asp Tyr Asn Leu Leu Asp Gln Ser 
65                  70                  75                  80  


Gln Ile Pro Gln Ser Thr Asn Pro Tyr Ala Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ala Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Lys Ile Asp Val Ile Asp Ser Asn Asp 
        115                 120                 125             


Asp Val Gly Asn Glu Leu Ser Thr Lys Glu Gln Leu Asn Lys Asn Ser 
    130                 135                 140                 


Lys Leu Leu Lys Asp Lys Phe Val Cys Gln Ile Gln Leu Glu Arg Met 
145                 150                 155                 160 


Asn Glu Gly Gln Val Arg Gly Glu Lys Asn Arg Phe Lys Thr Ala Asp 
                165                 170                 175     


Ile Ile Lys Glu Ile Ile Gln Leu Leu Asn Val Gln Lys Asn Phe His 
            180                 185                 190         


Gln Leu Asp Glu Asn Phe Ile Asn Lys Tyr Ile Glu Leu Val Glu Met 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro Tyr Gly Trp 
    210                 215                 220                 


Glu Gly Asp Pro Lys Ala Trp Tyr Glu Thr Leu Met Gly His Cys Thr 
225                 230                 235                 240 


Tyr Phe Pro Asp Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Gln Arg Asp 
            260                 265                 270         


Gly Leu Ser Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Asn Glu 
    290                 295                 300                 


Ile Asn Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser 
305                 310                 315                 320 


Gly Lys Pro Gln Phe Thr Glu Phe Lys Leu Tyr His Asp Leu Lys Ser 
                325                 330                 335     


Val Leu Phe Asp Gln Ser Ile Leu Glu Asn Glu Asp Val Leu Asp Gln 
            340                 345                 350         


Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile Lys Ser 
        355                 360                 365             


Lys Leu Thr Glu Leu Asp Ile Leu Leu Asn Glu Glu Asp Lys Glu Asn 
    370                 375                 380                 


Ile Ala Gln Leu Thr Gly Tyr Thr Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Cys Ile Arg Leu Val Leu Glu Glu Gln Trp Tyr Ser Ser Arg Asn Gln 
                405                 410                 415     


Met Glu Ile Phe Thr His Leu Asn Ile Lys Pro Lys Lys Ile Asn Leu 
            420                 425                 430         


Thr Ala Ala Asn Lys Ile Pro Lys Ala Met Ile Asp Glu Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Thr Phe Gly Gln Ala Ile Asn Leu Ile Asn 
    450                 455                 460                 


Lys Ile Ile Glu Lys Tyr Gly Val Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Lys Asp Lys Gln Lys Phe Ile Asn Glu Met 
                485                 490                 495     


Gln Lys Lys Asn Glu Asn Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly 
            500                 505                 510         


Lys Tyr Gly Asn Gln Asn Ala Lys Arg Leu Val Glu Lys Ile Arg Leu 
        515                 520                 525             


His Asp Glu Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Pro 
    530                 535                 540                 


Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn Lys Val Leu 
                565                 570                 575     


Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser Lys Lys Lys 
    610                 615                 620                 


Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Asn Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asn 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Tyr Leu Arg Lys 
        675                 680                 685             


Val Trp Lys Phe Lys Lys Glu Arg Asn His Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn 
705                 710                 715                 720 


Lys Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro Glu Ile Glu 
                725                 730                 735     


Ser Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn Tyr Ser Glu 
            740                 745                 750         


Met Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp Phe Arg Asn 
        755                 760                 765             


Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser Thr Tyr Ile 
785                 790                 795                 800 


Val Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr Thr Leu Lys 
                805                 810                 815     


Lys Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr Gln His Asp 
            820                 825                 830         


Pro Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln Tyr Ala Asn 
        835                 840                 845             


Glu Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly Glu Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys Ser Leu Lys 
865                 870                 875                 880 


Tyr Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr His Gln Phe 
                885                 890                 895     


Lys Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys Pro Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Thr Asp Lys Gly Tyr Lys Phe Ile Thr Ile Ser 
        915                 920                 925             


Tyr Leu Asp Val Leu Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln 
    930                 935                 940                 


Lys Tyr Asp Lys Leu Lys Leu Gly Lys Ala Ile Asp Lys Asn Ala Lys 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asp Gly Glu 
                965                 970                 975     


Ile Tyr Lys Ile Ile Gly Val Asn Ser Asp Thr Arg Asn Met Ile Glu 
            980                 985                 990         


Leu Asp Leu Pro Asp Ile Arg Tyr  Lys Glu Tyr Cys Glu  Leu Asn Asn 
        995                 1000                 1005             


Ile Lys  Gly Glu Pro Arg Ile  Lys Lys Thr Ile Gly  Lys Lys Val 
    1010                 1015                 1020             


Asn Ser  Ile Glu Lys Leu Thr  Thr Asp Val Leu Gly  Asn Val Phe 
    1025                 1030                 1035             


Thr Asn  Thr Gln Tyr Thr Lys  Pro Gln Leu Leu Phe  Lys Arg Gly 
    1040                 1045                 1050             


Asn 
    


<210>  533
<211>  1054
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  533

Met Lys Leu Thr Pro Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ala Val Val Glu Ile Asp Glu Gln Glu Asn Pro 
            20                  25                  30          


Leu Gly Leu Ile Asp Val Gly Val Arg Thr Phe Asp Arg Ala Glu Val 
        35                  40                  45              


Pro Lys Thr Gly Glu Ser Leu Ala Leu Ala Arg Arg Leu Ala Arg Ser 
    50                  55                  60                  


Ala Arg Arg Leu Val Lys Arg Arg Ala Asp Arg Ile Lys Lys Ala Lys 
65                  70                  75                  80  


Arg Leu Leu Lys Ala Glu Asn Ile Leu Leu Ser Ala Asp Glu His Leu 
                85                  90                  95      


Pro Asn Asp Val Trp Gln Leu Arg Val Lys Gly Leu Asp Gln Lys Leu 
            100                 105                 110         


Glu Arg Gln Glu Trp Ala Ala Val Leu Leu His Leu Leu Lys His Arg 
        115                 120                 125             


Gly Tyr Leu Ser Gln Arg Lys Asn Glu Ser Lys Ser Glu Asn Lys Glu 
    130                 135                 140                 


Leu Gly Ala Leu Leu Ser Gly Val Glu Thr Asn His Gln Ile Leu Gln 
145                 150                 155                 160 


Ser Ala Glu Tyr Arg Thr Pro Ala Glu Ile Ala Val Lys Lys Phe His 
                165                 170                 175     


Val Glu Asp Gly His Ile Arg Asn Gln Arg Gly Ala Tyr Thr His Thr 
            180                 185                 190         


Phe Ser Arg Leu Asp Leu Leu Ala Glu Met Glu Leu Leu Phe Gln Arg 
        195                 200                 205             


Gln Thr Asp Leu Gly Asn Pro His Thr Ser Ala Lys Leu Leu Glu Asn 
    210                 215                 220                 


Leu Thr Ala Leu Leu Met Trp Gln Lys Pro Ala Leu Ala Gly Glu Ala 
225                 230                 235                 240 


Ile Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Pro Thr Glu Tyr Lys 
                245                 250                 255     


Ala Ala Lys Asn Ser Tyr Ser Ala Glu Arg Phe Val Trp Leu Thr Lys 
            260                 265                 270         


Leu Asn Asn Leu Arg Ile Leu Glu Gln Gly Ala Glu Arg Ala Leu Thr 
        275                 280                 285             


Asp Asn Glu Arg Phe Ala Leu Leu Asp Gln Pro Tyr Glu Lys Ala Lys 
    290                 295                 300                 


Phe Thr Tyr Ala Gln Ala Arg Thr Met Leu Ala Leu Pro Asp Glu Ala 
305                 310                 315                 320 


Ile Phe Lys Gly Val Arg Tyr Gln Gly Glu Asp Lys Lys Ala Val Glu 
                325                 330                 335     


Thr Lys Thr Ile Leu Met Glu Met Lys Ala Tyr His Gln Ile Arg Lys 
            340                 345                 350         


Ala Leu Glu Asn Ala Asp Leu Lys Ala Glu Trp Asn Glu Leu Lys Asn 
        355                 360                 365             


Asn Ser Glu Leu Leu Asp Asp Ile Gly Thr Ala Phe Ser Leu His Lys 
    370                 375                 380                 


Thr Asp Glu Asp Ile Cys Arg Tyr Leu Asp Gly Lys Leu Ser Glu Ser 
385                 390                 395                 400 


Ile Leu Asn Ala Leu Leu Glu Asn Leu Asn Phe Asp Lys Phe Ile Gln 
                405                 410                 415     


Leu Ser Leu Lys Ala Leu Gln Gln Ile Leu Pro Leu Met Leu Gln Gly 
            420                 425                 430         


Gln Arg Tyr Asp Glu Ala Val Ser Ala Ile Tyr Gly Asp His Tyr Gly 
        435                 440                 445             


Lys Lys Ser Ala Glu Ile Asn Arg Leu Leu Pro Thr Ile Pro Ala Asp 
    450                 455                 460                 


Glu Ile Arg Asn Pro Val Val Leu Arg Thr Leu Thr Gln Ala Arg Lys 
465                 470                 475                 480 


Val Ile Asn Ala Val Val Arg Leu Tyr Gly Ser Pro Ala Arg Ile His 
                485                 490                 495     


Ile Glu Thr Gly Arg Glu Val Gly Lys Ser Tyr Gln Asp Arg Lys Lys 
            500                 505                 510         


Leu Glu Lys Gln Gln Glu Asp Asn Arg Lys Gln Arg Glu Ser Ala Val 
        515                 520                 525             


Lys Lys Phe Lys Glu Tyr Phe Pro Asn Phe Val Gly Glu Pro Lys Gly 
    530                 535                 540                 


Lys Asp Ile Leu Lys Met Arg Leu Tyr Glu Leu Gln Gln Ala Lys Cys 
545                 550                 555                 560 


Leu Tyr Ser Gly Lys Ser Leu Glu Leu His Arg Leu Leu Glu Lys Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp 
            580                 585                 590         


Ser Phe Asn Asn Lys Val Leu Val Leu Ala Asn Glu Asn Gln Asn Lys 
        595                 600                 605             


Gly Asn Leu Thr Pro Tyr Glu Trp Leu Asp Gly Lys Asn Asn Ser Glu 
    610                 615                 620                 


His Trp Gln Asn Phe Val Ala Arg Val Gln Thr Ser Gly Phe Ser His 
625                 630                 635                 640 


Thr Lys Lys Gln Arg Ile Leu Ser His Lys Leu Asp Glu Lys Gly Phe 
                645                 650                 655     


Ile Glu Arg Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe Leu Cys 
            660                 665                 670         


Asn Phe Ile Ala Asp Asn Met Leu Leu Thr Gly Lys Gly Lys Arg Lys 
        675                 680                 685             


Val Phe Ala Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg Gly Arg Trp 
    690                 695                 700                 


Gly Leu Gln Lys Val Arg Asp Asp Asn Asp Arg His His Ala Leu Asp 
705                 710                 715                 720 


Ala Val Val Val Ala Cys Ser Thr Val Val Met Gln Gln Lys Ile Thr 
                725                 730                 735     


Arg Phe Val Arg Tyr Glu Glu Gly Asn Val Phe Ser Gly Glu Arg Ile 
            740                 745                 750         


Asp Arg Glu Thr Gly Glu Ile Ile Pro Leu His Phe Pro Ser Pro Trp 
        755                 760                 765             


Ala Phe Phe Arg Glu Asn Val Glu Ile Arg Ile Phe Ser Glu Asn Pro 
    770                 775                 780                 


Lys Leu Glu Leu Glu Asn Arg Leu Pro Asp Tyr Pro Gln Tyr Asn His 
785                 790                 795                 800 


Glu Phe Val Gln Pro Leu Phe Val Ser Arg Met Pro Thr Arg Lys Met 
                805                 810                 815     


Thr Gly Gln Gly His Met Glu Thr Val Lys Ser Ala Lys Arg Leu Asp 
            820                 825                 830         


Glu Gly Leu Ser Val Leu Lys Val Pro Leu Thr Gln Leu Lys Leu Ser 
        835                 840                 845             


Asp Leu Glu Arg Met Val Asn Arg Glu Arg Glu Val Thr Leu Tyr Glu 
    850                 855                 860                 


Ser Leu Lys Ala Arg Leu Glu Gln Phe Gly Asn Asp Pro Ala Lys Ala 
865                 870                 875                 880 


Phe Ala Glu Pro Phe His Lys Lys Gly Gly Ala Val Val Lys Ala Val 
                885                 890                 895     


Arg Val Glu Gln Thr Gln Lys Ser Gly Val Leu Val Arg Asp Gly Asn 
            900                 905                 910         


Gly Val Ala Asp Asn Ala Ser Met Val Arg Val Asp Val Phe Thr Lys 
        915                 920                 925             


Gly Gly Lys Tyr Phe Leu Val Pro Ile Tyr Thr Trp Gln Val Ala Lys 
    930                 935                 940                 


Gly Ile Leu Pro Asn Lys Ala Val Thr Ala Asn Val Asp Glu Ile Asp 
945                 950                 955                 960 


Trp Leu Glu Met Asp Glu Ser Tyr Gln Phe Ile Phe Thr Met Tyr Pro 
                965                 970                 975     


Asn Asp Leu Val Lys Val Lys Leu Lys Lys Glu Glu Phe Phe Gly Tyr 
            980                 985                 990         


Tyr Gly Gly Leu Asp Arg Ala Thr  Gly Ala Ile Val Ile  Lys Glu His 
        995                 1000                 1005             


Asp Leu  Glu Lys Ser Lys Gly  Lys Gln Gly Ile Tyr  Arg Ile Gly 
    1010                 1015                 1020             


Val Lys  Leu Ala Leu Ser Phe  Glu Lys Tyr Gln Val  Asp Glu Leu 
    1025                 1030                 1035             


Gly Lys  Asn Ile Arg Pro Cys  Arg Pro Thr Lys Arg  Gln His Val 
    1040                 1045                 1050             


Arg 
    


<210>  534
<211>  1054
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  534

Met Lys Glu Lys Tyr Ile Leu Gly Leu Asp Leu Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asn Phe Glu Thr Lys Lys Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Asp Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Leu Leu Leu Thr Glu Tyr Asp Leu Ile Asn Lys Glu 
65                  70                  75                  80  


Gln Ile Pro Thr Ser Asn Asn Pro Tyr Gln Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ile Leu Ser Lys Asp Glu Leu Ala Ile Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Asn Ile Asn Val Ser Ser Glu Asp Glu 
        115                 120                 125             


Asp Ala Ser Asn Glu Leu Ser Thr Lys Glu Gln Ile Asn Arg Asn Asn 
    130                 135                 140                 


Lys Leu Leu Lys Asp Lys Tyr Val Cys Glu Val Gln Leu Gln Arg Leu 
145                 150                 155                 160 


Lys Glu Gly Gln Ile Arg Gly Glu Lys Asn Arg Phe Lys Thr Thr Asp 
                165                 170                 175     


Ile Leu Lys Glu Ile Asp Gln Leu Leu Lys Val Gln Lys Asp Tyr His 
            180                 185                 190         


Asn Leu Asp Ile Asp Phe Ile Asn Gln Tyr Lys Glu Ile Val Glu Thr 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Gln Gly Ser Pro Phe Gly Trp 
    210                 215                 220                 


Asn Gly Asp Leu Lys Lys Trp Tyr Glu Met Leu Met Gly His Cys Thr 
225                 230                 235                 240 


Tyr Phe Pro Gln Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Ile Ile Gln Arg Asp 
            260                 265                 270         


Asn Ser Glu Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu 
    290                 295                 300                 


Ile Gly Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser 
305                 310                 315                 320 


Gly Thr Pro Gln Phe Thr Glu Phe Lys Leu Tyr His Asp Leu Lys Ser 
                325                 330                 335     


Ile Val Phe Asp Lys Ser Ile Leu Glu Asn Glu Ala Ile Leu Asp Gln 
            340                 345                 350         


Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Glu Gln Ser Ile Lys Glu 
        355                 360                 365             


Glu Leu Asn Lys Leu Pro Glu Ile Leu Asn Glu Gln Asp Lys Ala Glu 
    370                 375                 380                 


Ile Ala Lys Leu Ile Gly Tyr Asn Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Cys Ile His Leu Ile Asn Glu Glu Leu Trp Gln Thr Ser Arg Asn Gln 
                405                 410                 415     


Met Glu Ile Phe Asn Tyr Leu Asn Ile Lys Pro Asn Lys Val Asp Leu 
            420                 425                 430         


Ser Glu Gln Asn Lys Ile Pro Lys Asp Met Val Asn Asp Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Thr Phe Ile Gln Ser Ile Asn Val Ile Asn 
    450                 455                 460                 


Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys Phe Ile Asn Asn Leu 
                485                 490                 495     


Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly 
            500                 505                 510         


Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile Val Glu Lys Ile Arg Leu 
        515                 520                 525             


His Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Ala 
    530                 535                 540                 


Leu Met Asp Leu Leu Asn Asn Pro Gln Asn Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ala Phe Asp Asn Ser Ile His Asn Lys Val Leu 
                565                 570                 575     


Val Lys Gln Ile Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Leu Asn Ser Ser Asp Ala Lys Leu Ser Tyr Asn Gln Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Ser Lys Asp Arg Ile Ser Lys Lys Lys 
    610                 615                 620                 


Lys Asp Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Ser Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asp 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asn His Leu Arg Lys 
        675                 680                 685             


Val Trp Arg Phe Asp Lys Tyr Arg Asn His Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn 
705                 710                 715                 720 


Lys Lys Leu Gln Asn Thr Asn Lys Ile Leu Glu Lys Pro Thr Ile Glu 
                725                 730                 735     


Asn Asn Thr Lys Lys Val Thr Val Glu Lys Glu Glu Asp Tyr Asn Asn 
            740                 745                 750         


Val Phe Glu Thr Pro Lys Leu Val Glu Asp Ile Lys Gln Tyr Arg Asp 
        755                 760                 765             


Tyr Lys Phe Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Met Lys Asp Glu His Asp Tyr Ile 
785                 790                 795                 800 


Val Gln Thr Ile Thr Asp Ile Tyr Gly Lys Asp Asn Thr Asn Leu Lys 
                805                 810                 815     


Lys Gln Phe Asn Lys Asn Pro Glu Lys Phe Leu Met Tyr Gln Asn Asp 
            820                 825                 830         


Pro Lys Thr Phe Glu Lys Leu Ser Ile Ile Met Lys Gln Tyr Ser Asp 
        835                 840                 845             


Glu Lys Asn Pro Leu Ala Lys Tyr Tyr Glu Glu Thr Gly Glu Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys Lys Ile Lys 
865                 870                 875                 880 


Leu Leu Gly Asn Lys Val Gly Asn His Leu Asp Val Thr Asn Lys Tyr 
                885                 890                 895     


Glu Asn Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys Asn Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Thr Glu Lys Gly Tyr Lys Phe Val Thr Ile Ala 
        915                 920                 925             


Tyr Leu Asn Val Phe Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Lys Asp 
    930                 935                 940                 


Lys Tyr Gln Glu Leu Lys Glu Lys Lys Lys Ile Lys Asp Thr Asp Gln 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asn Gly Asp 
                965                 970                 975     


Leu Tyr Lys Ile Ile Gly Val Asn Ser Asp Asp Arg Asn Ile Ile Glu 
            980                 985                 990         


Leu Asp Tyr Tyr Asp Ile Lys Tyr  Lys Asp Tyr Cys Glu  Ile Asn Asn 
        995                 1000                 1005             


Ile Lys  Gly Glu Pro Arg Ile  Lys Lys Thr Ile Gly  Lys Lys Thr 
    1010                 1015                 1020             


Glu Ser  Ile Glu Lys Phe Thr  Thr Asp Val Leu Gly  Asn Leu Tyr 
    1025                 1030                 1035             


Leu His  Ser Thr Glu Lys Ala  Pro Gln Leu Ile Phe  Lys Arg Gly 
    1040                 1045                 1050             


Leu 
    


<210>  535
<211>  1054
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  535

Met Glu Asn Lys Asn Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ala Val Val Glu Ile Asp Glu Lys Glu Asn Pro 
            20                  25                  30          


Leu Arg Leu Ile Asp Val Gly Val Arg Thr Phe Glu Arg Ala Glu Val 
        35                  40                  45              


Pro Lys Thr Gly Glu Ser Leu Ala Leu Ser Arg Arg Leu Ala Arg Ser 
    50                  55                  60                  


Ala Arg Arg Leu Thr Gln Arg Arg Val Ala Arg Leu Lys Lys Ala Lys 
65                  70                  75                  80  


Arg Leu Leu Lys Ser Glu Asn Ile Leu Leu Ser Thr Asp Glu Arg Leu 
                85                  90                  95      


Pro His Gln Val Trp Gln Leu Arg Val Glu Gly Leu Asp His Lys Leu 
            100                 105                 110         


Glu Arg Gln Glu Trp Ala Ala Val Leu Leu His Leu Ile Lys His Arg 
        115                 120                 125             


Gly Tyr Leu Ser Gln Arg Lys Asn Glu Ser Lys Ser Glu Asn Lys Glu 
    130                 135                 140                 


Leu Gly Ala Leu Leu Ser Gly Val Asp Asn Asn His Lys Leu Leu Gln 
145                 150                 155                 160 


Gln Ala Thr Tyr Arg Ser Pro Ala Glu Leu Ala Val Lys Lys Phe Glu 
                165                 170                 175     


Val Glu Glu Gly His Ile Arg Asn Gln Gln Gly Ala Tyr Thr His Thr 
            180                 185                 190         


Phe Ser Arg Leu Asp Leu Leu Ala Glu Met Glu Leu Leu Phe Ser Arg 
        195                 200                 205             


Gln Gln His Phe Gly Asn Pro Phe Ala Ser Glu Lys Leu Leu Glu Asn 
    210                 215                 220                 


Leu Thr Ala Leu Leu Met Trp Gln Lys Pro Ala Leu Ser Gly Glu Ala 
225                 230                 235                 240 


Ile Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Asp Glu Tyr Lys Ala 
                245                 250                 255     


Ala Lys Asn Thr Tyr Ser Ala Glu Arg Phe Val Trp Ile Thr Lys Leu 
            260                 265                 270         


Asn Asn Leu Arg Ile Gln Glu Asn Gly Leu Glu Arg Ala Leu Asn Asp 
        275                 280                 285             


Asn Glu Arg Leu Ala Leu Met Glu Gln Pro Tyr Asp Lys Asn Arg Leu 
    290                 295                 300                 


Phe Tyr Ser Gln Val Arg Ser Ile Leu Lys Leu Ser Asp Glu Ala Ile 
305                 310                 315                 320 


Phe Lys Gly Leu Arg Tyr Ser Gly Glu Asp Lys Lys Ala Ile Glu Thr 
                325                 330                 335     


Lys Ala Val Leu Met Glu Met Lys Ala Tyr His Gln Ile Arg Lys Val 
            340                 345                 350         


Leu Glu Gly Asn Asn Leu Lys Ala Glu Trp Ala Glu Leu Lys Ala Asn 
        355                 360                 365             


Pro Thr Leu Leu Asp Glu Ile Gly Thr Ala Phe Ser Leu Tyr Lys Thr 
    370                 375                 380                 


Asp Glu Asp Ile Ser Ala Tyr Leu Ala Gly Lys Leu Ser Gln Pro Val 
385                 390                 395                 400 


Leu Asn Ala Leu Leu Glu Asn Leu Ser Phe Asp Lys Phe Ile Gln Leu 
                405                 410                 415     


Ser Leu Lys Ala Leu Tyr Lys Leu Leu Pro Leu Met Gln Gln Gly Leu 
            420                 425                 430         


Arg Tyr Asp Glu Ala Cys Arg Glu Ile Tyr Gly Asp His Tyr Gly Lys 
        435                 440                 445             


Lys Thr Glu Glu Asn His His Phe Leu Pro Gln Ile Pro Ala Asp Glu 
    450                 455                 460                 


Ile Arg Asn Pro Val Val Leu Arg Thr Leu Thr Gln Ala Arg Lys Val 
465                 470                 475                 480 


Ile Asn Gly Val Val Arg Leu Tyr Gly Ser Pro Ala Arg Ile His Ile 
                485                 490                 495     


Glu Thr Gly Arg Glu Val Gly Lys Ser Tyr Lys Asp Arg Arg Glu Leu 
            500                 505                 510         


Glu Lys Arg Gln Glu Glu Asn Arg Lys Gln Arg Glu Asn Ala Ile Lys 
        515                 520                 525             


Glu Phe Lys Glu Tyr Phe Pro His Phe Ala Gly Glu Pro Lys Ala Lys 
    530                 535                 540                 


Asp Ile Leu Lys Met Arg Leu Tyr Lys Gln Gln Asn Ala Lys Cys Leu 
545                 550                 555                 560 


Tyr Ser Gly Lys Pro Ile Glu Leu His Arg Leu Leu Glu Lys Gly Tyr 
                565                 570                 575     


Val Glu Val Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser 
            580                 585                 590         


Phe Asn Asn Lys Val Leu Val Leu Ala Asn Glu Asn Gln Asn Lys Gly 
        595                 600                 605             


Asn Leu Thr Pro Phe Glu Trp Leu Asp Gly Lys His Asn Ser Glu Arg 
    610                 615                 620                 


Trp Arg Ala Phe Lys Ala Leu Val Glu Thr Ser Ala Phe Pro Tyr Ala 
625                 630                 635                 640 


Lys Lys Gln Arg Ile Leu Ser Gln Lys Leu Asp Glu Lys Gly Phe Ile 
                645                 650                 655     


Glu Arg Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe Leu Cys Asn 
            660                 665                 670         


Phe Ile Ala Asp Asn Met His Leu Thr Gly Glu Gly Lys Arg Lys Val 
        675                 680                 685             


Phe Ala Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg Ser Arg Trp Gly 
    690                 695                 700                 


Leu Ala Lys Ser Arg Glu Asp Asn Asp Arg His His Ala Leu Asp Ala 
705                 710                 715                 720 


Val Val Val Ala Cys Ser Thr Val Ala Met Gln Gln Lys Ile Thr Arg 
                725                 730                 735     


Phe Val Arg Phe Glu Ala Gly Asp Val Phe Thr Gly Glu Arg Ile Asp 
            740                 745                 750         


Arg Glu Thr Gly Glu Ile Ile Pro Leu His Phe Pro Thr Pro Trp Gln 
        755                 760                 765             


Phe Phe Lys Gln Glu Val Glu Ile Arg Ile Phe Ser Asp Asn Pro Lys 
    770                 775                 780                 


Leu Glu Leu Glu Asn Arg Leu Pro Asp Arg Pro Gln Ala Asn His Glu 
785                 790                 795                 800 


Phe Val Gln Pro Leu Phe Val Ser Arg Met Pro Thr Arg Lys Met Thr 
                805                 810                 815     


Gly Gln Gly His Met Glu Thr Val Lys Ser Ala Lys Arg Leu Asn Glu 
            820                 825                 830         


Gly Ile Ser Val Ile Lys Met Pro Leu Thr Lys Leu Lys Leu Lys Asp 
        835                 840                 845             


Leu Glu Leu Met Val Asn Arg Glu Arg Glu Lys Asp Leu Tyr Asp Thr 
    850                 855                 860                 


Leu Lys Ala Arg Leu Glu Ala Phe Asn Asp Asp Pro Ala Lys Ala Phe 
865                 870                 875                 880 


Ala Glu Pro Phe Ile Lys Lys Gly Gly Ala Ile Val Lys Ser Val Arg 
                885                 890                 895     


Val Glu Gln Ile Gln Lys Ser Gly Val Leu Val Arg Glu Gly Asn Gly 
            900                 905                 910         


Val Ala Asp Asn Ala Ser Met Val Arg Val Asp Val Phe Thr Lys Gly 
        915                 920                 925             


Gly Lys Tyr Phe Leu Val Pro Ile Tyr Thr Trp Gln Val Ala Lys Gly 
    930                 935                 940                 


Ile Leu Pro Asn Lys Ala Ala Thr Gln Tyr Lys Asp Glu Glu Asp Trp 
945                 950                 955                 960 


Glu Val Met Asp Asn Ser Ala Thr Phe Lys Phe Ser Leu His Pro Asn 
                965                 970                 975     


Asp Leu Val Lys Leu Val Thr Lys Lys Lys Thr Ile Leu Gly Tyr Phe 
            980                 985                 990         


Asn Gly Leu Asn Arg Ala Thr Gly  Asn Ile Asp Ile Lys  Glu His Asp 
        995                 1000                 1005             


Leu Asp  Lys Ser Lys Gly Lys  Gln Gly Ile Phe Glu  Gly Val Gly 
    1010                 1015                 1020             


Ile Lys  Leu Ala Leu Ser Phe  Glu Lys Tyr Gln Val  Asp Glu Leu 
    1025                 1030                 1035             


Gly Lys  Asn Ile Arg Leu Cys  Lys Pro Ser Lys Arg  Gln Pro Val 
    1040                 1045                 1050             


Arg 
    


<210>  536
<211>  1055
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  536

Met Asn Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly 
1               5                   10                  15      


Tyr Gly Ile Val Asp Ser Asp Thr Arg Glu Ile Lys Asp Ala Gly Val 
            20                  25                  30          


Arg Leu Phe Pro Glu Ala Asn Val Asp Asn Asn Glu Gly Arg Arg Ser 
        35                  40                  45              


Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu Asp 
    50                  55                  60                  


Arg Val Lys His Leu Leu Ala Glu Tyr Asn Leu Leu Asp Leu Thr Asn 
65                  70                  75                  80  


Ile Pro Lys Ser Thr Asn Pro Tyr Gln Ile Arg Val Lys Gly Leu Asn 
                85                  90                  95      


Glu Lys Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile His Asn Val Asn Val Met Met Asp Asp Asn Asp 
        115                 120                 125             


Ser Gly Asn Glu Leu Ser Thr Lys Asp Gln Leu Lys Lys Asn Ala Lys 
    130                 135                 140                 


Ala Leu Ser Asp Lys Tyr Val Cys Glu Leu Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Gln Asp Tyr Lys Val Arg Gly Glu Lys Asn Arg Phe Lys Thr Glu Asp 
                165                 170                 175     


Phe Val Arg Glu Ala Arg Lys Leu Leu Glu Thr Gln Ser Lys Phe Phe 
            180                 185                 190         


Glu Ile Asp Gln Thr Phe Ile Met Arg Tyr Ile Asp Leu Val Glu Thr 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro Phe Gly Trp 
    210                 215                 220                 


Glu Gly Asn Ile Lys Lys Trp Phe Glu Gln Met Met Gly His Cys Thr 
225                 230                 235                 240 


Tyr Phe Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Glu 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp 
            260                 265                 270         


Glu Glu Ala Lys Leu Asn Tyr Gly Glu Lys Phe Gln Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Thr Pro Asn Leu Lys Gln Ile Ala Lys Glu 
    290                 295                 300                 


Ile Gly Val Ser Glu Thr Asp Ile Lys Gly Tyr Arg Val Asn Lys Ser 
305                 310                 315                 320 


Gly Lys Pro Glu Phe Thr Gln Phe Lys Leu Tyr His Asp Leu Lys Asn 
                325                 330                 335     


Ile Phe Glu Asp Ser Lys Tyr Leu Asn Asp Val Gln Leu Met Asp Asn 
            340                 345                 350         


Ile Ala Glu Ile Ile Thr Ile Tyr Gln Asp Pro Glu Ser Ile Ile Lys 
        355                 360                 365             


Glu Leu Asn Leu Leu Pro Glu Leu Leu Ser Glu Lys Glu Lys Glu Lys 
    370                 375                 380                 


Ile Ser Ala Leu Ser Gly Tyr Ala Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Cys Ile Asn Leu Leu Leu Asp Asp Leu Trp Glu Ser Ser Leu Asn Gln 
                405                 410                 415     


Met Glu Leu Phe Thr Lys Leu Asn Leu Lys Pro Lys Lys Ile Asp Leu 
            420                 425                 430         


Ser Gln Gln His Lys Ile Pro Ile Lys Leu Val Asp Asp Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Ala Phe Ile Gln Ser Ile Gln Val Val Asn 
    450                 455                 460                 


Ala Ile Ile Asp Lys Tyr Gly Leu Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Asp Asp Arg Arg Lys Phe Leu Asn Gln Leu 
                485                 490                 495     


Gln Lys Gln Asn Ala Glu Thr Arg Lys Gln Val Glu Lys Val Leu Arg 
            500                 505                 510         


Glu Tyr Gly Asn Asp Asn Ala Lys Arg Ile Val Gln Lys Ile Lys Leu 
        515                 520                 525             


His Asn Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Lys Asp Ile Pro 
    530                 535                 540                 


Leu Glu Asp Leu Leu Lys Asn Pro Asn His Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ala Phe Asp Asn Ser Met His Asn Lys Val Leu 
                565                 570                 575     


Val Arg Ala Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Leu Asn Ser Ser Glu Ser Ser Leu Ser Tyr Asn Glu Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Thr Lys Asp Arg Ile Thr Lys Lys Lys 
    610                 615                 620                 


Arg Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Tyr Asp Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Ser Leu Leu Lys Ala Tyr Phe Ser Ala Asn Asn Leu Asp 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asn Tyr Leu Arg Lys 
        675                 680                 685             


Val Trp Lys Phe Asp Lys Asp Arg Asn Lys Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys His Asn 
705                 710                 715                 720 


Lys Lys Leu Arg Asn Ile Asn Lys Val Leu Asp Ala Pro Ser Lys Glu 
                725                 730                 735     


Ile Asp Lys Lys Arg Val Thr Val Gln Ser Glu Asp Glu Tyr Asn Gln 
            740                 745                 750         


Met Phe Glu Asp Thr Gln Lys Ala Gln Ala Ile Lys Lys Phe Glu Ile 
        755                 760                 765             


Arg Lys Phe Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Lys Asp Thr Leu Tyr Ser Thr Arg Asn Ile Asp Gly Ile Glu Tyr Val 
785                 790                 795                 800 


Val Glu Ser Ile Lys Asp Ile Tyr Ser Val Asn Asn Asp Lys Val Lys 
                805                 810                 815     


Thr Lys Phe Lys Lys Asp Pro His Arg Leu Leu Met Tyr Arg Asn Asp 
            820                 825                 830         


Pro Gln Thr Phe Glu Lys Phe Glu Lys Val Phe Lys Gln Tyr Glu Ser 
        835                 840                 845             


Glu Lys Asn Pro Phe Ala Lys Tyr Tyr Glu Glu Thr Gly Glu Lys Ile 
    850                 855                 860                 


Arg Lys Phe Ser Lys Thr Gly Gln Gly Pro Tyr Ile Asn Lys Ile Lys 
865                 870                 875                 880 


Tyr Leu Arg Glu Arg Leu Gly Arg His Cys Asp Val Thr Asn Lys Tyr 
                885                 890                 895     


Ile Asn Ser Arg Asn Lys Ile Val Gln Leu Lys Ile Tyr Ser Tyr Arg 
            900                 905                 910         


Phe Asp Ile Tyr Gln Tyr Gly Asn Asn Tyr Lys Met Ile Thr Ile Ser 
        915                 920                 925             


Tyr Ile Asp Leu Glu Gln Lys Ser Asn Tyr Tyr Tyr Ile Ser Arg Glu 
    930                 935                 940                 


Lys Tyr Glu Gln Lys Lys Lys Asp Lys Gln Ile Asp Asp Ser Tyr Lys 
945                 950                 955                 960 


Phe Ile Gly Ser Phe Tyr Lys Asn Asp Ile Ile Asn Tyr Asn Gly Glu 
                965                 970                 975     


Met Tyr Arg Val Ile Gly Val Asn Asp Ser Glu Lys Asn Lys Ile Gln 
            980                 985                 990         


Leu Asp Met Ile Asp Ile Ser Ile  Lys Asp Tyr Met Glu  Leu Asn Asn 
        995                 1000                 1005             


Ile Lys  Lys Thr Gly Val Ile  Tyr Lys Thr Ile Gly  Lys Ser Thr 
    1010                 1015                 1020             


Thr His  Ile Glu Lys Tyr Thr  Thr Asp Ile Leu Gly  Asn Leu Tyr 
    1025                 1030                 1035             


Lys Ala  Ala Pro Pro Lys Lys  Pro Gln Leu Ile Phe  Lys Arg Gly 
    1040                 1045                 1050             


Met Glu  
    1055 


<210>  537
<211>  1055
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  537

Met Thr Pro Ile Ser Glu Ser Leu Leu Phe Gly Leu Asp Val Gly Ile 
1               5                   10                  15      


Ser Ser Cys Gly Trp Ala Val Leu Lys His Ser Asp Thr Thr Gly His 
            20                  25                  30          


Ile Glu Ala Leu Gly Ser Trp Cys Phe Asn Ala Pro Glu Thr Lys Thr 
        35                  40                  45              


Glu Arg Thr Pro Thr Asn Gln Ile Arg Arg Ser Ser Arg Leu Leu Arg 
    50                  55                  60                  


Arg Val Val Arg Arg Arg Arg Asn Arg Met Thr Glu Ile Arg Arg Leu 
65                  70                  75                  80  


Phe Ala Arg His Gly Leu Ile Asp Asn Asp Arg Pro Asp Ala Leu Arg 
                85                  90                  95      


Gln Pro Ala Ile Asp Pro Trp Glu Met Arg Gly Gln Gly Leu Asp Arg 
            100                 105                 110         


Gln Leu Thr Pro Glu Glu Phe Ala Val Ala Leu Ala His Ile Ala Lys 
        115                 120                 125             


His Arg Gly Phe Lys Ser Ser Ala Lys Arg Val Lys Ser Asn Ala Ser 
    130                 135                 140                 


Pro Asp Asp Ser Lys Met Leu Ala Ala Leu Glu Lys Thr Lys Glu Leu 
145                 150                 155                 160 


Ser Ala Gln Tyr Arg Thr Ile Gly Glu Met Phe Ala Arg Asp Ser Ala 
                165                 170                 175     


Tyr Val Gln Arg Arg Arg Asn Arg Asp Gly Leu Phe Asp Arg Thr Ile 
            180                 185                 190         


Ser Arg Asp Asp Leu Ile Asp Glu Ala Lys Ile Leu Phe Asp Arg Gln 
        195                 200                 205             


Arg Lys Leu Gly Asn Pro Val Ala Ser Thr Glu Ile Glu Glu Ala Phe 
    210                 215                 220                 


Thr Arg Ile Ala Phe Trp Gln Asn Pro Met Gln Asp Ser Gly Val Leu 
225                 230                 235                 240 


Val Ala Ser Cys Pro Phe Glu Pro Lys Glu Lys Arg Ser Ser Arg Phe 
                245                 250                 255     


Ser Pro Ser Phe Glu Arg Phe Arg Leu Leu Cys Arg Leu Ile Asn Leu 
            260                 265                 270         


Arg Val Thr Asp Gly Pro Glu Glu Arg Pro Leu Thr Glu Glu Glu Leu 
        275                 280                 285             


Arg Leu Ala Tyr Glu His Ala Gly Lys Thr Arg Lys Leu Thr Ala Ala 
    290                 295                 300                 


Ala Ile Arg Lys Leu Ile Gly Leu Arg Asp Asp Gln His Phe Thr Thr 
305                 310                 315                 320 


Ile Pro Pro Glu Gln Glu Lys Gln Asp Ile Thr Ser Arg Thr Gly Glu 
                325                 330                 335     


Thr Phe Ala Gly Thr Ala Thr Phe Arg Ser Val Leu Gly Glu Ser Leu 
            340                 345                 350         


Trp Arg Gln Leu Leu Pro His Pro Glu Trp Leu Asp Asn Ala Ala Trp 
        355                 360                 365             


Ile Ile Ser Phe His Glu Leu Thr Glu Thr Ile Ile Lys Lys Leu Gly 
    370                 375                 380                 


Glu Ile Gly Leu Pro Val Glu Ala Tyr Glu Leu Ile Cys Asp Ala Leu 
385                 390                 395                 400 


Lys Ser Asp Lys Ser Pro Phe Ala Lys Phe Lys Gly Ala Ser Ser Leu 
                405                 410                 415     


Ser Asp Lys Ala Val Arg Ala Leu Ile Pro Tyr Leu Gln Gln Gly Leu 
            420                 425                 430         


Thr Tyr Asp Lys Ala Cys Gln Lys Ala Gly Tyr Asp His Ala Ala Ser 
        435                 440                 445             


Arg Phe Ser Gly Arg Ala Thr Ile Asp Thr Lys Ala Lys Phe Asn Ala 
    450                 455                 460                 


Leu Met Ala Glu Val Arg Asp Ser Ile Ala Asn Pro Ile Ala Arg Lys 
465                 470                 475                 480 


Ala Leu Ser Glu Gly Met Lys Gln Leu Trp Ala Met Arg Asn Arg Trp 
                485                 490                 495     


Gly Leu Pro Gly Ala Ile Cys Ile Glu Leu Ala Arg Asp Val Gly Asn 
            500                 505                 510         


Ser Leu Glu Lys Arg Lys Glu Ile Glu Arg Asn Leu Lys Thr Thr Ala 
        515                 520                 525             


Lys Gln Arg Ala Arg Glu Arg Glu Glu Ala Cys Glu Leu Leu Asn Leu 
    530                 535                 540                 


Lys Glu Val Ser Ser Asp Ile Leu Leu Arg Tyr Arg Leu Trp Lys Glu 
545                 550                 555                 560 


Gln Gly Gly Arg Cys Cys Tyr Ser Gly Lys Ser Ile Thr Pro Asp Met 
                565                 570                 575     


Leu Met Ala Gln Asp Asn Arg Ile Gln Ile Asp His Ile Leu Pro Trp 
            580                 585                 590         


Ser Arg Phe Gly Asp Asp Ser Tyr Ser Asn Lys Gly Leu Cys Leu Ala 
        595                 600                 605             


Lys Glu Asn Gln Ala Lys Lys Asn Gln Thr Pro Tyr Glu Trp Ile His 
    610                 615                 620                 


Gly Gln Gly Thr Thr Ala Gln Lys Glu Gln Trp Asp Lys Phe Val Ala 
625                 630                 635                 640 


Asp Val Glu Ser Arg Lys Asn Thr Lys Gly Leu Lys Lys Arg Asn Phe 
                645                 650                 655     


Leu Leu Lys Gly Ser Lys Glu Ile Glu Glu Arg Phe Arg Ser Arg Asn 
            660                 665                 670         


Leu Asn Asp Thr Arg Tyr Ala Ala Arg Leu Met Val Glu Ala Ala Arg 
        675                 680                 685             


Leu Leu Tyr Pro Ala Gly Gln Arg Gly Glu Lys Gly Gly Thr Arg Arg 
    690                 695                 700                 


Val Phe Thr Arg Pro Gly Ala Leu Thr Ala Ala Leu Arg His Ala Trp 
705                 710                 715                 720 


Gly Val Glu Ser Leu Lys Lys Ile Asp Gly Glu Arg Leu His Asp Asn 
                725                 730                 735     


Arg His His Ala Leu Asp Ala Ala Ile Val Ala Ala Val Ser Glu Ala 
            740                 745                 750         


Glu Ile Gln Lys Leu Thr Lys Ser Phe Gln Glu Cys Glu Gln Lys Gly 
        755                 760                 765             


Leu Ser Arg Pro Met Arg Asp Val Ala Pro Pro Trp Asp Gly Phe Arg 
    770                 775                 780                 


Asp Gln Leu Lys Glu Lys Tyr Glu Thr Ile Phe Val Ala Arg Pro Glu 
785                 790                 795                 800 


Arg Gln Arg Ala Arg Gly Gln Gly His Glu Ala Thr Ile Arg Gln Val 
                805                 810                 815     


Arg Glu Glu Glu Thr Gly Pro Val Ile Tyr Glu Arg Lys Ala Val Ser 
            820                 825                 830         


Ala Leu Thr Leu Ala Asp Leu Ala Lys Ile Lys Asp Pro Asp Arg Asn 
        835                 840                 845             


Ala Gly Leu Ile Ala Ser Leu Arg Gln Trp Ile Glu Ala Gly Lys Pro 
    850                 855                 860                 


Ala Asp Lys Pro Pro Arg Thr Pro Cys His Gln Ser Ala Ala Gly His 
865                 870                 875                 880 


Glu Ile Arg Lys Val Arg Leu Ala Thr Ile Ile Thr Pro Ala Val Pro 
                885                 890                 895     


Val Arg Gly Gly Met Ala Lys Arg Gly Glu Met Thr Arg Val Asp Ile 
            900                 905                 910         


Phe Lys Val Ile Gly Lys Lys Gly Lys Pro Glu Trp Tyr Met Ile Pro 
        915                 920                 925             


Ile Tyr Pro His Gln Ile Met Asn Arg Ser Gln Trp Pro Ala Pro Pro 
    930                 935                 940                 


Asn Lys Ala Val Val Ala Gly Lys Ser Glu Ala Asp Trp Pro Ile Met 
945                 950                 955                 960 


Gly Ala Glu Ala Gln Phe Gln Phe Ser Leu Tyr Pro Cys Ser Tyr Val 
                965                 970                 975     


Arg Leu Phe Lys Glu Gly Thr Val Ile Glu Gly Tyr Phe Cys Gly Cys 
            980                 985                 990         


His Arg Ser Arg Ala Tyr Ile Thr  Leu Ala Leu Ser Thr  Gly Ala Ala 
        995                 1000                 1005             


Pro Leu  Gln Lys Gly Ile Gly  Ile Lys Thr Gln Glu  His Ile Gln 
    1010                 1015                 1020             


Lys Phe  Ser Val Asn Arg Phe  Gly Glu Leu Ser Pro  Val Lys Gln 
    1025                 1030                 1035             


Glu Thr  Arg Thr Trp His Gly  Val Ala Cys Thr Ser  Pro Asp Gln 
    1040                 1045                 1050             


His Asp  
    1055 


<210>  538
<211>  1056
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  538

Met Lys Ala Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile Ala Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Val Glu Ile Asp Gln Asn Glu Leu Pro Ile Arg 
            20                  25                  30          


Leu Ile Asp Leu Gly Val Arg Thr Phe Glu Arg Ala Glu Val Pro Lys 
        35                  40                  45              


Thr Gly Asp Ser Leu Ala Leu Ala Arg Arg Leu Ala Arg Ser Ser Arg 
    50                  55                  60                  


Arg Leu Val Arg Arg Arg Ala Val Arg Leu Leu Lys Ala Lys Arg Leu 
65                  70                  75                  80  


Leu Lys Gln Glu Gly Ile Leu Gln Ala Asp Asp Phe His Ser Ser His 
                85                  90                  95      


Leu Lys Pro Leu Pro Asn Asn Pro Trp Glu Leu Arg Val Lys Gly Leu 
            100                 105                 110         


Asn His Leu Leu Asp Arg Lys Glu Trp Ala Ala Val Leu Leu His Leu 
        115                 120                 125             


Leu Lys His Arg Gly Tyr Leu Ser Gln Arg Lys Asn Glu Ser Lys Thr 
    130                 135                 140                 


Asp Asn Lys Glu Leu Gly Ala Leu Leu Lys Gly Val Ser Glu Asn Arg 
145                 150                 155                 160 


Gln Leu Leu Gln Ala Gln Ala Phe Ser Thr Pro Ala Glu Ile Ala Ile 
                165                 170                 175     


Lys Lys Phe Gln Gln Glu Glu Gly His Ile Arg Asn Gln Arg Gly Ala 
            180                 185                 190         


Tyr Ser His Thr Phe Asp Arg Asn Asp Leu Leu Ala Glu Leu Arg Leu 
        195                 200                 205             


Leu Phe Gln Lys Gln Arg Glu Phe Asn Asn Pro Tyr Thr Ser Gln Ser 
    210                 215                 220                 


Leu Glu Gln Ala Ile Ala Glu Leu Leu Met Trp Gln Lys Pro Ala Leu 
225                 230                 235                 240 


Ser Gly Glu Ala Ile Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Pro 
                245                 250                 255     


Ser Glu Tyr Lys Ala Pro Lys Asn Ser Tyr Ser Ala Glu Tyr Phe Val 
            260                 265                 270         


Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile Leu Glu Asn Gly Ala Asp 
        275                 280                 285             


Arg Gly Leu Thr Ala Glu Glu Arg Gln Leu Leu Ile Asn Gln Pro Phe 
    290                 295                 300                 


Glu Lys Ala Lys Phe Thr Tyr Ala Gln Ala Arg Lys Leu Leu Glu Leu 
305                 310                 315                 320 


Ser Asp Leu Ala Ile Phe Lys Gly Val Arg Tyr Gly Lys Asp Asn Ala 
                325                 330                 335     


Glu Ser Ala Thr Leu Met Glu Met Lys Ala Trp His Ala Ile Arg Lys 
            340                 345                 350         


Ala Leu Glu Ser Asn Gly Leu Lys Thr Glu Trp Asn Gly Leu Lys Thr 
        355                 360                 365             


Lys Pro Glu Leu Leu Asp Lys Ile Gly Thr Ala Phe Ser Ile Tyr Lys 
    370                 375                 380                 


Thr Asp Glu Asp Ile Ser Gln Tyr Leu Asn Gly Glu Leu Ser Pro Ala 
385                 390                 395                 400 


Val Leu Asn Ala Leu Leu Glu Asn Leu Ser Phe Asp Lys Phe Ile Arg 
                405                 410                 415     


Leu Ser Leu Gln Ser Leu Ala Lys Ile Leu Pro Leu Met Glu Gln Gly 
            420                 425                 430         


Leu Arg Tyr Asp Glu Ala Cys Lys Gln Val Tyr Gly Asp His Tyr Gly 
        435                 440                 445             


Lys Lys Glu Gln Leu Glu Leu Arg Phe Leu Pro Lys Ile Pro Val Glu 
    450                 455                 460                 


Asp Ile Arg Asn Pro Val Val Leu Arg Thr Leu Ser Gln Ala Arg Lys 
465                 470                 475                 480 


Val Ile Asn Gly Val Val Arg Leu Tyr Gly Ser Pro Ala Arg Ile His 
                485                 490                 495     


Ile Glu Thr Gly Arg Glu Val Gly Lys Ser Phe Ser Glu Arg Arg Asp 
            500                 505                 510         


Ile Ser Lys Arg Gln Glu Glu Asn Arg Asn Glu Arg Gln Arg Ala Val 
        515                 520                 525             


Glu Lys Phe Lys Glu Phe Phe Pro His Phe Val Gly Glu Pro Lys Ala 
    530                 535                 540                 


Lys Asp Ile Leu Lys Met Arg Leu Tyr Glu Gln Gln His Gly Lys Cys 
545                 550                 555                 560 


Leu Tyr Ser Gly Lys Glu Leu Asp Leu Asn Arg Leu Leu Glu Lys Gly 
                565                 570                 575     


Tyr Val Glu Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp 
            580                 585                 590         


Ser Phe Asn Asn Lys Val Leu Val Leu Ala Ser Glu Asn Gln Asn Lys 
        595                 600                 605             


Gly Asn Gln Thr Pro Tyr Glu Trp Leu Asn Gly Lys Glu Asn Ala Glu 
    610                 615                 620                 


Arg Trp Lys Leu Phe Gln Ala Leu Val Asn Gly Ser Arg Phe Pro Tyr 
625                 630                 635                 640 


Ser Lys Lys Gln Arg Ile Leu Thr Lys Tyr Leu Asp Glu Asp Gly Phe 
                645                 650                 655     


Arg Glu Arg Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe Leu Cys 
            660                 665                 670         


Asn Phe Ile Glu Asp Asn Met Gln Leu Val Gly Gln Gly Lys Lys Arg 
        675                 680                 685             


Val Phe Ala Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg Arg Ser Trp 
    690                 695                 700                 


Gly Ile Gln Lys Val Arg Glu Asp Asn Asp Arg His His Ala Val Asp 
705                 710                 715                 720 


Ala Val Val Val Ala Cys Ser Thr Arg Ala Leu Gln Lys Lys Ile Thr 
                725                 730                 735     


Asp Tyr Val Arg Tyr Lys Glu Met Asn Val Phe Thr Gly Glu Lys Ile 
            740                 745                 750         


Asp Gln Glu Thr Gly Glu Ile Ile Lys Phe His Phe Pro Glu Pro Trp 
        755                 760                 765             


Lys Phe Phe Arg Gln Glu Val Glu Ile Arg Val Phe Ser Glu Thr Pro 
    770                 775                 780                 


Ala Glu Asp Val Val Ile Ala Leu Pro Asp Arg Pro Gln Ala Asn His 
785                 790                 795                 800 


Glu Phe Ile Gln Pro Leu Phe Val Ser Arg Ala Pro Thr Arg Lys Met 
                805                 810                 815     


Thr Gly Gln Gly His Met Glu Thr Ile Lys Ser Ala Lys Arg Leu Ala 
            820                 825                 830         


Glu Gly Leu Ser Val Ser Arg Val Pro Leu Thr Gln Leu Lys Leu Lys 
        835                 840                 845             


Asp Leu Glu Asn Met Val Asn Arg Glu Arg Glu Lys Ala Leu Tyr Glu 
    850                 855                 860                 


Ala Leu Lys Val Arg Leu Glu Lys Phe Asn Asn Asp Pro Ala Lys Ala 
865                 870                 875                 880 


Phe Ala Asp Pro Phe Tyr Lys Lys Gly Gly Gln Gln Val Lys Ala Val 
                885                 890                 895     


Arg Val Glu Gln Val Gln Lys Ser Gly Val Leu Val Arg Gln Glu Asn 
            900                 905                 910         


Gly Val Ala Asp Asn Ala Ser Met Val Arg Val Asp Val Phe Ile Lys 
        915                 920                 925             


Gly Gly Lys Tyr Phe Leu Val Pro Ile Tyr Thr Trp Gln Val Ala Lys 
    930                 935                 940                 


Gly Ile Leu Pro Asn Arg Ala Ala Thr Gln Gly Lys Glu Glu Glu Tyr 
945                 950                 955                 960 


Trp Asp Ile Met Asp Glu Thr Ala Ile Phe Gln Phe Ser Leu Tyr Gln 
                965                 970                 975     


Asn Asp Leu Ile Lys Leu Val Thr Lys Lys Lys Thr Ile Phe Gly Tyr 
            980                 985                 990         


Phe Asn Gly Leu Asn Arg Ala Thr  Ser Asn Ile Asn Ile  Lys Glu His 
        995                 1000                 1005             


Asp Leu  Asp Lys Ser Lys Gly  Lys Asp Gly Ile Tyr  Leu Glu Val 
    1010                 1015                 1020             


Gly Val  Lys Leu Ala Leu Ser  Leu Glu Lys Tyr Gln  Val Asp Glu 
    1025                 1030                 1035             


Leu Gly  Lys Asn Ile Gln Pro  Cys Arg Ser Met Lys  Lys Arg Pro 
    1040                 1045                 1050             


Pro Val  Arg 
    1055     


<210>  539
<211>  1056
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  539

Met Pro Lys Gln Lys Ser Thr Arg Tyr Arg Leu Ala Leu Asp Leu Gly 
1               5                   10                  15      


Ser Thr Ser Leu Gly Trp Ala Val Leu Arg Leu Asn Glu Gln His Glu 
            20                  25                  30          


Pro Asn Ala Ile Ile Arg Ala Gly Val Arg Ile Phe Ser Asn Gly Arg 
        35                  40                  45              


Asn Pro Lys Asp Gly Ser Ser Leu Ala Val Thr Arg Arg Ala Ala Arg 
    50                  55                  60                  


Ala Met Arg Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Ala Arg Met 
65                  70                  75                  80  


Met Asn Lys Leu Val Glu Tyr Gly Phe Phe Pro Ala Asp Val Ala Glu 
                85                  90                  95      


Arg Lys Lys Leu Glu Arg Ile Asn Pro Tyr Glu Leu Arg Ala Lys Gly 
            100                 105                 110         


Leu Asp Ala Pro Leu Thr Pro Gly Glu Phe Ala Arg Ala Leu Phe His 
        115                 120                 125             


Ile Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Lys 
    130                 135                 140                 


Glu Asn Asp Ser Gly Ala Leu Lys Ala Ala Ile Ser His Leu Arg Gln 
145                 150                 155                 160 


Gln Leu His Glu Arg Lys Ala Arg Thr Val Gly Glu Trp Leu Trp Gln 
                165                 170                 175     


Arg Leu Gln Ala Gly Leu Gly Thr Arg Ala Arg Tyr Arg Glu Ser Arg 
            180                 185                 190         


Val Ala Gln Ala Asn Gly Lys Thr Arg Ile Glu Lys Ser Tyr Asp Leu 
        195                 200                 205             


Tyr Val Asp Arg Gln Met Ile Ala Glu Glu Phe Asp Ala Leu Trp Ala 
    210                 215                 220                 


Ala Gln Ala Ala Phe Asn Pro Gln Gln Phe Ser Glu Gln Ala Arg Gly 
225                 230                 235                 240 


Glu Leu Arg Asp Val Leu Leu Phe Gln Arg Asp Leu Arg Pro Val Arg 
                245                 250                 255     


Pro Gly Arg Cys Thr Leu Leu Pro Asp Glu Glu Arg Ala Pro Leu Ala 
            260                 265                 270         


Leu Pro Ser Val Gln Arg Phe Arg Ile Leu Gln Glu Ala Asn His Leu 
        275                 280                 285             


Arg Leu Leu His Pro Asp Leu Arg Glu Glu Pro Leu Ser Leu Ala Gln 
    290                 295                 300                 


Arg Asp Ala Val Val Asp Ala Leu Glu His Asn Ser Lys Arg Thr Phe 
305                 310                 315                 320 


Glu Gln Leu Arg Lys Leu Leu Lys Leu Asn Gly Thr Val Lys Phe Asn 
                325                 330                 335     


Leu Glu Asp Glu Lys Arg Lys Glu Leu Lys Gly Asn Ala Thr Ser Ala 
            340                 345                 350         


Thr Leu Ser Lys Asp Gly Leu Phe Gly Glu Asn Trp Trp Asn Leu Asp 
        355                 360                 365             


Glu Ala Leu Gln Asp Ala Ile Val Trp Gln Leu Val Ser Glu Gln Ser 
    370                 375                 380                 


Glu Glu Lys Leu Gln Asn Trp Leu Gln Ala Asn Thr Gly Val Asn Ala 
385                 390                 395                 400 


Thr Gln Ala Glu Thr Ile Ala Asn Ala Gly Leu Pro Glu Gly Tyr Gly 
                405                 410                 415     


Ser Leu Ser Tyr Lys Ala Leu Ala Arg Ile Ile Pro Ala Leu Arg Gly 
            420                 425                 430         


Thr Glu Val Ala Asn Gly Ile Asp Val Pro Arg Thr Tyr Asp Gln Ala 
        435                 440                 445             


Val Gln Ala Ala Gly Phe Ala His His Ser Asp Leu Asp Ser Gly Phe 
    450                 455                 460                 


Asp Phe Glu His Ser His Asp Glu Val Glu Arg Ile Gly Glu Arg Val 
465                 470                 475                 480 


Ile Ala Ser Thr Gly Glu Val Lys Pro Val Phe Ala Phe Lys Gln Leu 
                485                 490                 495     


Pro Tyr Tyr Gly Lys Ala Leu Arg Arg His Val Ala Phe Ala Lys Glu 
            500                 505                 510         


His Pro Arg Asn Glu Glu Glu Arg Tyr Gly Lys Ile Ala Asn Pro Thr 
        515                 520                 525             


Val His Ile Gly Leu Asn Gln Leu Arg Val Val Val Asn Ala Leu Ile 
    530                 535                 540                 


Arg Arg Tyr Gly Arg Pro Thr Glu Val Val Val Glu Leu Ala Arg Asp 
545                 550                 555                 560 


Leu Lys Gln Ser Arg Glu Gln Arg Gln Glu Glu Gln Lys Arg Gln Ala 
                565                 570                 575     


Asp Tyr Gln Lys Arg Asn Glu His Ile Arg Arg Glu Ile Ala Ser Met 
            580                 585                 590         


His Gly Cys Ala Glu Glu Arg Val Ser His Ala Asp Val Gln Lys Trp 
        595                 600                 605             


Ile Leu Trp Glu Glu Leu Ser Phe Asp Val Ala Asp Arg Arg Cys Pro 
    610                 615                 620                 


Tyr Ser Gly Val Gln Ile Ser Ala Gln Met Leu Leu Ser Asn Gln Val 
625                 630                 635                 640 


Glu Ile Glu His Ile Leu Pro Phe Ser Gln Thr Leu Asp Asn Ser Leu 
                645                 650                 655     


Asn Asn Arg Thr Val Ala Met Arg Gln Ala Asn Arg Ile Lys Gly Asn 
            660                 665                 670         


Arg Thr Pro Trp Gln Ala Arg Glu Asp Phe Glu Arg Gln Gly Trp Ser 
        675                 680                 685             


Tyr Asp Ser Ile Leu Gln Arg Ala Glu Arg Met Pro Lys Ala Lys Arg 
    690                 695                 700                 


Tyr Arg Phe Ser Arg Asp Gly Tyr Glu Arg Trp Leu Lys Glu Asp Lys 
705                 710                 715                 720 


Asp Phe Leu Ala Arg Ala Leu Asn Asp Thr Arg Tyr Leu Ser Arg Val 
                725                 730                 735     


Ala Lys Thr Tyr Met Glu Leu Val Cys Pro Leu Gly Thr Arg Val Ile 
            740                 745                 750         


Pro Gly Gln Met Thr Ala Leu Leu Arg Ala Lys Phe Gly Leu Asn Asp 
        755                 760                 765             


Val Leu Gly Leu Asn Gly Glu Lys Asn Arg Asp Asp His Arg His His 
    770                 775                 780                 


Ala Val Asp Ala Cys Val Ile Gly Val Thr Asp Gln Gly Leu Leu Gln 
785                 790                 795                 800 


Arg Phe Ala Gln Ala Ser Ala Asp Ala Arg Gly Asn Gly Leu Glu Lys 
                805                 810                 815     


Leu Val Glu Asn Met Pro Val Pro Trp Ala Thr Tyr Arg Asp His Val 
            820                 825                 830         


Lys Arg Ala Ile Gln Cys Ile Trp Val Ser His Lys Pro Asp His Gly 
        835                 840                 845             


Tyr Glu Gly Pro Met Met Glu Glu Thr Ala Tyr Gly Ile Arg Lys Asp 
    850                 855                 860                 


Gly Ser Ile Arg Gln Lys Thr Lys Ala Asp Gly Ser Thr Gly Arg Glu 
865                 870                 875                 880 


Ile Thr His Leu Ile Arg Ile Thr Glu Pro Thr Gln Asn Leu Arg His 
                885                 890                 895     


Gly Val Asp Ala Gln Gly Gln Pro Leu Ala Tyr Lys Gly Tyr Val Gly 
            900                 905                 910         


Gly Ser Asn Asp Cys Ile Glu Ile Thr Gln Asn Glu Gln Gly Lys Trp 
        915                 920                 925             


Glu Gly Asn Val Ile Thr Thr Phe Gln Ala Tyr Asp Ile Val Arg Arg 
    930                 935                 940                 


Leu Gly Ser Lys Gln Gly Pro Arg Arg Leu Arg His Pro Thr Leu Ala 
945                 950                 955                 960 


Gln Thr Leu Asp Val Tyr Gly Lys Pro Lys Lys Leu Val Met Arg Leu 
                965                 970                 975     


Met Ile Asp Asp Cys Val Arg Leu Glu Leu Asp Gly Asn Lys Lys Thr 
            980                 985                 990         


Leu Arg Val Val Lys Ile Thr Gly  Asn Gly Gln Val Phe  Met Ala Pro 
        995                 1000                 1005             


Val His  Glu Ala Asn Val Asp  Lys Arg Asn Ser Asp  Lys Thr Asp 
    1010                 1015                 1020             


Pro Phe  Ala Tyr Val Ser Lys  Tyr Ala Gly Ser Phe  Gln Lys Ala 
    1025                 1030                 1035             


Lys Ala  Arg Arg Ile Thr Ile  Ser Pro Ile Gly Glu  Leu His Asp 
    1040                 1045                 1050             


Pro Gly  Phe 
    1055     


<210>  540
<211>  1057
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  540

Met Ser Asp Lys Ser Lys Ile Leu Gly Phe Asp Ile Gly Ile Thr Ser 
1               5                   10                  15      


Ile Gly Trp Ala Tyr Val Glu Gly Glu Glu Leu Ile Asp Cys Gly Val 
            20                  25                  30          


Arg Ile Phe Thr Lys Ala Glu Asn Pro Lys Thr Lys Glu Ser Leu Ala 
        35                  40                  45              


Leu Pro Arg Arg Glu Ala Arg Gly Val Arg Arg Arg Leu Ala Arg Arg 
    50                  55                  60                  


Lys Gly Arg Leu Asn Ala Ile Lys Gln Leu Ile Cys Lys Glu Phe Gly 
65                  70                  75                  80  


Leu Asn Tyr Gln Asp Tyr Ile Ala Gln Asp Gly Glu Leu Pro Lys Ala 
                85                  90                  95      


Tyr Ala Gly Lys Leu Ile Asn Pro Tyr Glu Leu Arg Ser Lys Ala Leu 
            100                 105                 110         


Arg Glu Lys Leu Glu Lys Glu Glu Leu Ala Arg Val Ile Leu His Ile 
        115                 120                 125             


Ala Lys His Arg Gly Tyr Gly Asn Lys His Ala Lys Glu Thr Asp Asp 
    130                 135                 140                 


Ala Glu Ser Gly Lys Ile Lys Lys Ala Ile Ser Asn Asn Glu Lys Ile 
145                 150                 155                 160 


Leu Asn Glu Lys Gly Tyr Gln Ser Ala Gly Glu Tyr Leu Tyr Gln Glu 
                165                 170                 175     


Phe Phe Gln Lys Glu Arg Leu Gln Glu Glu Lys Lys Gly Asn Asn Thr 
            180                 185                 190         


Thr Lys Glu Phe Lys Asn Val Arg Asn Lys Ala Gly Asp Tyr Glu His 
        195                 200                 205             


Cys Ile Ala Gln Thr Gln Met Gln Asn Glu Leu Arg Leu Ile Phe Glu 
    210                 215                 220                 


Lys Gln Arg Glu Phe Gly Leu Asn Phe Ser Glu Glu Phe Lys Lys Lys 
225                 230                 235                 240 


Val Leu Glu Thr Ala Phe Phe Gln Arg Pro Leu Lys Asp Phe Ser Asp 
                245                 250                 255     


Lys Ile Gly Asp Cys Thr Phe Ile Glu Gly Ala Lys Arg Ala Pro Lys 
            260                 265                 270         


Asp Ser Leu Ser Ala Ile Glu Phe Val Ala Leu Gly Arg Ile Ile Asn 
        275                 280                 285             


Thr Leu Lys Asn Leu Gln Asn Thr Ser Gly Glu Val Tyr Ser Arg Asp 
    290                 295                 300                 


Lys Ile Asn Glu Ile Leu Gln Ile Val Leu Asp Lys Gly Glu Ile Ser 
305                 310                 315                 320 


Tyr Lys Lys Leu Arg Glu Ile Leu Asp Ile Glu Ser Ser Val Arg Phe 
                325                 330                 335     


Lys Asp Ser Lys Leu Asp Tyr Thr Lys Glu Leu Lys Glu Ala Glu Lys 
            340                 345                 350         


Thr Lys Leu Phe Glu Ser Lys Asn Leu Gln Lys Phe Lys Lys Ala Leu 
        355                 360                 365             


Gly Ser Phe Glu Gly Ile Ser Arg Glu Ile Leu Asp Ser Ile Ala Lys 
    370                 375                 380                 


Glu Ile Met Ile Thr Lys Asp Lys Glu Lys Leu Ala Lys Glu Leu Lys 
385                 390                 395                 400 


Lys Phe Pro Leu Asp Glu Thr Gln Ile Gln Asn Leu Ser Asn Leu Ser 
                405                 410                 415     


Phe Ser Gln Thr Ile Asn Leu Ser Leu Glu Ala Leu Ser Gln Ile Leu 
            420                 425                 430         


Pro Phe Met Arg Gly Glu Arg Glu Asp Lys Cys Leu Arg Tyr Asp Glu 
        435                 440                 445             


Ala Val Glu Lys Ala Gly Leu Lys Glu Val Ala Lys Asn Lys Ser Lys 
    450                 455                 460                 


Asp Asp Phe Leu Pro Pro Leu Glu Asn Tyr Glu Pro Tyr Leu Ala Asn 
465                 470                 475                 480 


Pro Val Val Ala Arg Ala Leu Ser Glu Tyr Arg Lys Val Leu Asn Ala 
                485                 490                 495     


Leu Leu Arg Lys Tyr Gly Lys Met His Lys Ile His Ile Glu Phe Thr 
            500                 505                 510         


Arg Glu Val Gly Lys Ser Lys Glu Glu Arg Asn Lys Ile Glu Lys Glu 
        515                 520                 525             


Gln Lys Glu Asn Phe Ala Lys Asn Gln Glu Ala Gln Lys Gln Cys Glu 
    530                 535                 540                 


Ile Leu Gly Leu Pro Ile Asn Gly Ala Asn Ile Leu Lys Met Lys Leu 
545                 550                 555                 560 


Trp Arg Glu Gln Gln Glu Ser Cys Val Tyr Ser Gly Lys Glu Ile Ala 
                565                 570                 575     


Ile Asp Asp Leu Lys Asn Pro Ala Ile Leu Gln Val Asp His Ile Tyr 
            580                 585                 590         


Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Asn Asn Lys Val Leu Val 
        595                 600                 605             


Phe Ser Lys Glu Asn Gln Asn Lys Leu Asn Lys Thr Pro Phe Glu Ala 
    610                 615                 620                 


Phe Gly Glu Asn Lys Asp Lys Trp Glu Lys Ile Leu Ser Leu Ala Ser 
625                 630                 635                 640 


Lys Leu Pro Lys Pro Lys Arg Gln Arg Ile Tyr Asn Met Gln Phe Lys 
                645                 650                 655     


Asp Lys Glu Gln Gly Phe Leu Thr Arg Asn Ile Asn Asp Thr Gly Tyr 
            660                 665                 670         


Ile Ala Arg Leu Val Ser Gln Trp Thr Lys Asn Cys Leu Ser Phe Glu 
        675                 680                 685             


Lys Leu Ser Glu Asp Glu Val Ser Ile Ala Gly Glu Lys Gly Ser Lys 
    690                 695                 700                 


Val His Val Glu Val Ile Ser Gly Ser Leu Thr Ala Met Leu Arg His 
705                 710                 715                 720 


Tyr Trp Gly Leu Gly Asp Lys Asn Arg Asp Asn His Leu His His Ala 
                725                 730                 735     


Val Asp Ala Ile Ile Ile Ala Tyr Ser Asn Glu Lys Val Ile Lys Ala 
            740                 745                 750         


Phe Ser Asp Tyr Lys Lys His Arg Glu Gln Asn Lys Ala Lys Phe Tyr 
        755                 760                 765             


Ala Lys Glu Ile Ala Glu Ser Glu Tyr Lys Lys Gln Lys Val Phe Phe 
    770                 775                 780                 


Glu Pro Thr Asn His Phe Arg Gln Lys Val Leu Glu Lys Ile Asp Ser 
785                 790                 795                 800 


Ile Phe Val Ser Lys Pro Pro Arg Lys Arg Ala Arg Gly Ala Leu His 
                805                 810                 815     


Glu Glu Thr Phe Tyr Ser Phe Glu Arg Leu Cys Asn Asp Lys Glu Tyr 
            820                 825                 830         


Ala Lys Lys Glu Lys Met Asp Tyr Gly Gly Lys Ala Gly Ile Gln Lys 
        835                 840                 845             


Ala Ile Ala Leu Gly Lys Ile Arg Gln Val Gly Thr Lys Ile Val Ser 
    850                 855                 860                 


Asn Gly Thr Met Val Arg Val Asp Ile Phe Gln His Lys Ser Asn Gly 
865                 870                 875                 880 


Lys Phe Tyr Gly Val Pro Ile Tyr Thr Met Asp Phe Ala Leu Gly Val 
                885                 890                 895     


Leu Pro Asn Lys Ala Val Val Ser Gly Lys Asp Lys Gln Gly Val Ile 
            900                 905                 910         


Lys Asp Trp Leu Glu Met Asp Ser Asn Tyr Glu Phe Cys Phe Ser Leu 
        915                 920                 925             


Phe Lys Asp Asp Leu Ile Leu Val Gln Lys Lys Glu Met Glu Lys Ala 
    930                 935                 940                 


Glu Leu Cys Tyr Tyr Thr Asp Phe Asn Ser Asn Thr Ala Gly Ile Lys 
945                 950                 955                 960 


Phe Glu Lys His Asn Asn Leu Tyr Val Asn Leu Asn Glu Asn Glu Arg 
                965                 970                 975     


Lys Leu Phe Thr Ser Lys Glu Val Val Lys Gly Gly Lys Lys Asn Val 
            980                 985                 990         


Trp Lys Asp Lys Thr Pro Lys Glu  Ile Glu Gln Leu Glu  Lys Asn Arg 
        995                 1000                 1005             


Lys Ile  Ile Gly Gly Phe Val  Thr Ile Glu Asp Leu  Lys Thr Phe 
    1010                 1015                 1020             


Glu Lys  Tyr Gln Val Ser Pro  Leu Gly Glu Val Gln  Lys Ala Asp 
    1025                 1030                 1035             


Phe Ala  Pro Arg Gln Ala Ile  Thr Leu Lys Ser Ser  Pro Lys His 
    1040                 1045                 1050             


Arg Ile  Asn Lys 
    1055         


<210>  541
<211>  1057
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  541

Met Ser Asn Arg Val Pro Tyr Arg Leu Ala Leu Asp Leu Gly Thr Asn 
1               5                   10                  15      


Ser Ile Gly Trp Cys Leu Leu Asp Leu Asn Gly Asn Gly Asp Pro Cys 
            20                  25                  30          


Gly Val Arg Arg Met Gly Val Arg Ile Phe Pro Asp Gly Arg Asp Pro 
        35                  40                  45              


Lys Ser Lys Glu Ser Leu Ala Ala Asp Arg Arg Leu Ala Arg Ser Met 
    50                  55                  60                  


Arg Arg Arg Arg Asp Arg Tyr Leu Arg Arg Arg Ser Ala Leu Met Asn 
65                  70                  75                  80  


Ala Leu Val Ala Ala Gly Leu Met Pro Ala Asp Arg Ala Thr Arg Lys 
                85                  90                  95      


Ala Leu Glu Ser Glu Asp Pro Cys Glu Leu Arg Ala Arg Ala Leu Phe 
            100                 105                 110         


Glu Pro Leu Thr Ala Gly Gln Val Gly Arg Ala Leu Phe His Leu Asn 
        115                 120                 125             


Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Ala Ala Gly Glu Glu Asn 
    130                 135                 140                 


Glu Ala Gly Lys Ile Lys Ala Gly Ile Ser Ala Leu Lys Ala Glu Ile 
145                 150                 155                 160 


Glu Ala Ser Gly Glu Thr Thr Leu Gly Ala Trp Leu Ala Ala Arg His 
                165                 170                 175     


Arg Lys Arg Glu Thr Val Arg Ala Arg Leu Asp Gly Ala Gly Ala Lys 
            180                 185                 190         


Ala Ser Tyr Glu Phe Tyr Pro Glu Arg Gly Leu Val His Asp Glu Phe 
        195                 200                 205             


Gln Ala Ile Arg Thr Ala Gln Ala Leu His His Pro Ala Val Thr Ala 
    210                 215                 220                 


Glu Gln Trp Asp Arg Val Glu Ser Val Ile Phe Arg Gln Arg Pro Leu 
225                 230                 235                 240 


Arg Pro Val Arg Pro Gly Lys Cys Thr Leu Phe Pro Asp Gln Glu Arg 
                245                 250                 255     


Ala Pro Trp Ala Leu Pro Val Ala Gln Glu Phe Arg Ile Leu Thr Glu 
            260                 265                 270         


Ile Gly Asn Leu Lys Leu Tyr Arg Pro Gly Glu Glu Pro Arg Glu Leu 
        275                 280                 285             


Ser Ala Asp Gln His Ala Gln Leu Phe Ala Lys Leu Cys Arg Ser Ala 
    290                 295                 300                 


Asp Leu Thr Phe Glu Lys Ile Arg Lys Glu Leu Lys Leu Asp Pro Gly 
305                 310                 315                 320 


Leu Lys Val Asn Leu Glu Gly Glu Lys Arg Asp Arg Leu Lys Gly Asp 
                325                 330                 335     


Ala Val Thr Ser Ala Leu Ser Ala Lys Lys Arg Phe Gly Pro Lys Trp 
            340                 345                 350         


Asn Ala Leu Thr Pro Asp Asp Arg Arg Asp Ile Val Gly Arg Leu Ile 
        355                 360                 365             


Glu Ala Glu Asp Glu Ala Glu Leu Ala Gly Trp Leu Glu Ser Val His 
    370                 375                 380                 


Gly Leu Pro Ser Glu Asn Ala Ala His Thr Ala Ala Ser Pro Gly Leu 
385                 390                 395                 400 


Pro Asp Gly Tyr Cys Arg Leu Ser Glu Lys Ala Leu Val Lys Leu Val 
                405                 410                 415     


Pro Leu Met Arg Glu Gln Arg Leu Arg Tyr Asp Glu Ala Val Leu Ala 
            420                 425                 430         


Leu Asn Leu His His Ser Asp Phe Arg Pro Glu Gly Leu Leu Asp Glu 
        435                 440                 445             


Leu Pro Tyr Tyr Gly Glu Pro Leu Glu Arg Phe Ile Ala Phe Gly Ser 
    450                 455                 460                 


Gly Glu Pro Ser Asp Pro Pro Glu Gln His Leu Gly Arg Phe Pro Asn 
465                 470                 475                 480 


Pro Thr Val His Ile Gly Leu Asn Glu Leu Arg Lys Leu Val Asn Arg 
                485                 490                 495     


Ile Val Glu Arg Trp Gly Lys Pro Ala Asp Ile Val Val Glu Leu Ala 
            500                 505                 510         


Arg Asp Leu Lys Arg Ser Arg Asp Glu Arg Asp Thr Ile Gln Arg Glu 
        515                 520                 525             


Gln Ala Gln Asn Gln Arg Lys Asn Glu Asp Arg Phe Ala Lys Ile Lys 
    530                 535                 540                 


Glu Leu Gly Ile Asp Pro Arg Pro Asp Ser Met Leu Arg Leu Arg Leu 
545                 550                 555                 560 


Trp Glu Glu Leu Pro Lys Val Gly Val Gly His Val Cys Val Tyr Thr 
                565                 570                 575     


Gly Glu Pro Ile Gly Ile Arg Arg Leu Leu Glu Gly Asp Val Asp Ile 
            580                 585                 590         


Asp His Ile Leu Pro Phe Ser Arg Thr Leu Ser Asp Gly Ala Asp Asn 
        595                 600                 605             


Lys Ile Val Cys Val Arg Arg Ala Asn Arg Phe Lys Arg Asp Arg Ser 
    610                 615                 620                 


Pro Trp Glu Ala Phe Gly Val Thr Ala Val Asp Gly Tyr Asp Trp Ala 
625                 630                 635                 640 


Ala Ile Ala Gln Arg Ala Glu Glu Leu Pro Lys Asn Lys Arg Trp Arg 
                645                 650                 655     


Phe Leu Pro Asp Ala Met Asp Arg Phe Glu Gly Glu Arg Gly Phe Leu 
            660                 665                 670         


Asp Arg Gln Leu Val Glu Thr Arg His Leu Ser Arg Val Ala Arg Gln 
        675                 680                 685             


Tyr Leu Ala Ser Ile Val Asp Leu Asn Lys Val Arg Val Ala Pro Gly 
    690                 695                 700                 


Lys Leu Thr Ala Met Leu Arg Ala Arg Trp Gly Leu Asn Lys Ile Leu 
705                 710                 715                 720 


Ser Asp Gly Asn Leu Lys Asn Arg Ser Asp His Arg His His Ala Ile 
                725                 730                 735     


Asp Ala Ala Val Leu Gly Met Ile Asp Gln Arg Leu Val Gln Leu Val 
            740                 745                 750         


Ala Gln Ala Ala Ala Arg Ala Glu Ala Asn Glu Leu Glu Arg Leu Phe 
        755                 760                 765             


Asp Gly Leu Pro Asp Pro Phe Asp Gly Leu Ala Ala Thr Ser Phe Gly 
    770                 775                 780                 


Asp Gln Val Arg Glu Lys Ile Arg Gly Ile Thr Val Ser His Arg Ala 
785                 790                 795                 800 


Glu His Gly Trp Gln Gly Arg Met His Asn Asp Thr Ala Tyr Gly Pro 
                805                 810                 815     


Val Glu Ala Val Asn Gly Val His Thr Val Val Thr Arg Lys Pro Leu 
            820                 825                 830         


Ser Gly Leu Thr Ala Lys Asp Phe Pro Asn Ile Arg Asp Arg Val Leu 
        835                 840                 845             


Ala Lys Ala Leu Glu Asp His Val Ala Ala Glu Ala Ala Ala Gly Arg 
    850                 855                 860                 


Ser Phe Gly Asp Ala Leu Gly Ser Phe Lys Trp Gly Gly Gln Pro Val 
865                 870                 875                 880 


Arg Arg Val Arg Met Leu Asp Val Leu Gly Asn Pro Ile Pro Ile Gln 
                885                 890                 895     


Asp Pro Ser Gly Thr Val Phe Lys Val Tyr Lys Gly Asp Ser Asn Ala 
            900                 905                 910         


Trp Tyr Glu Ile Leu Arg Asp Pro Arg Gly Arg Trp Thr Gly Lys Ile 
        915                 920                 925             


Val Ser Thr Phe Glu Ala Tyr Arg Leu His Arg Glu Trp Leu Ala Glu 
    930                 935                 940                 


Thr Gly Gly Asp Ser Gly Arg Gly Ser Gly Val Phe Leu Asp Trp Ala 
945                 950                 955                 960 


Ala Lys Arg Val Val Gly Glu Ala Gly Thr Pro Leu Phe Arg Leu Phe 
                965                 970                 975     


Lys Gly Asp Leu Leu Ala Leu Gly Glu Ala Pro Asp Arg Arg Ile Val 
            980                 985                 990         


Arg Val Val Lys Phe Ser Glu Gly  Gln Val Ala Leu Ala  Leu His Leu 
        995                 1000                 1005             


Glu Ala  Gly Asn Leu Lys Ala  Arg Asp Ala Asp Lys  Gly Asp Pro 
    1010                 1015                 1020             


Phe Lys  Tyr Gln Tyr Cys Ser  Pro Asp Ser Leu Arg  Lys Gln Ala 
    1025                 1030                 1035             


Ala Arg  Pro Ala Gly Val Asp  Leu Leu Gly Tyr Val  His Asp Lys 
    1040                 1045                 1050             


Gly Pro  Leu Glu 
    1055         


<210>  542
<211>  1057
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  542

Met Arg Arg Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly Trp Cys 
1               5                   10                  15      


Leu Ile Glu Asp Asp Lys Lys Ile Val Asp Ile Gly Val Arg Ile Phe 
            20                  25                  30          


Ser Asp Gly Arg Asp Pro Lys Ser Gly Ala Ser Leu Ala Val Asp Arg 
        35                  40                  45              


Arg Asp Ala Arg Ala Met Arg Arg Arg Arg Asp Arg Tyr Leu Gly Arg 
    50                  55                  60                  


Arg Ser Ala Phe Leu Gln Ala Leu Val Val His Gly Leu Met Pro Ala 
65                  70                  75                  80  


Asp Ala Asp Glu Ala Lys Leu Val Ala Asp Arg Asp Pro Tyr Gln Leu 
                85                  90                  95      


Arg Ala Lys Ala Leu His Val Ala Leu Glu Pro Phe Glu Ile Gly Arg 
            100                 105                 110         


Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys 
        115                 120                 125             


Ala Asp Arg Lys Ala Asn Ala Gly Glu Asp Gly Lys Ile Ala Glu Gly 
    130                 135                 140                 


Ala Lys Ala Leu Asp Gln Ala Met Ser Glu Ala Gly Val Asp Thr Leu 
145                 150                 155                 160 


Gly Gln Phe Leu Ala Leu Lys Glu Thr Lys Arg Val Arg Leu Gly Ser 
                165                 170                 175     


Asp Gly Gln Ala Tyr Asp Phe Tyr Pro Gln Arg Arg His Ile Glu Tyr 
            180                 185                 190         


Glu Phe Asp Arg Ile Trp Lys Val Gln Ser Glu Tyr His Pro Ala Leu 
        195                 200                 205             


Leu Thr Glu Ala Ala Arg Ala Ala Leu His Arg Ile Leu Phe Phe Gln 
    210                 215                 220                 


Arg Pro Leu Lys Ala Gln Glu Val Gly Phe Cys Thr Phe Ala Gly Val 
225                 230                 235                 240 


Asn Gly Ile Ala Ala Asn Glu Arg Arg Leu Pro Lys Ala His Pro Leu 
                245                 250                 255     


Phe Gln Gln Arg Arg Leu Tyr Glu Glu Val Asn Gln Leu Arg Ile Val 
            260                 265                 270         


Ser Ala Gly Ala Ala Asp Arg Pro Leu Thr Leu Asp Glu Arg Asp Arg 
        275                 280                 285             


Leu Ile Leu Arg Leu Gln Asp Arg Lys Lys Val Ser Phe Asp Thr Leu 
    290                 295                 300                 


Ala Lys Lys Asp Leu Arg Leu Ala Asp Gly Glu Arg Phe Asn Lys Glu 
305                 310                 315                 320 


Ser Glu Asn Arg Lys Glu Leu Ile Gly Asp Glu Val Arg Ala Glu Met 
                325                 330                 335     


Ser Asp Lys Lys Arg Phe Gly Thr Arg Trp Val His Phe Gly Leu Asp 
            340                 345                 350         


Glu Gln Leu Arg Ile Leu Ala Lys Leu Gln Asp Glu Glu Asn Pro Glu 
        355                 360                 365             


Ala Leu Leu Asp Trp Leu Arg Ser Glu Tyr Gly Leu Asp Glu Ala Ala 
    370                 375                 380                 


Ala Glu Ser Val Ala Asn Ala Arg Leu Pro Glu Gly Tyr Gly Arg Phe 
385                 390                 395                 400 


Gly Glu Thr Ala Thr Arg Leu Leu Leu Asp Ala Leu Lys Ala Glu Val 
                405                 410                 415     


Ile Thr Tyr Asp Lys Ala Ala Leu Arg Ala Phe Gly His His Ser Asp 
            420                 425                 430         


Phe Arg Thr Gly Glu Val Leu Asp Arg Leu Pro Tyr Tyr Gly Glu Leu 
        435                 440                 445             


Leu Thr Arg Glu Ile Ala Pro Gly Lys Pro Glu Tyr Gly Asp Pro Leu 
    450                 455                 460                 


Glu Arg Gln Tyr Gly Lys Ile Thr Asn Pro Thr Val His Ile Gly Leu 
465                 470                 475                 480 


Arg Gln Leu Glu Lys Leu Val Asn Ala Val Ile Ala Val His Gly Arg 
                485                 490                 495     


Pro Asp Gln Ile Val Val Glu Leu Ala Arg Glu Leu Lys Leu Asn Glu 
            500                 505                 510         


Lys Glu Lys Asp Glu His Lys Arg Arg Ile Lys Arg Asp Thr Asp Ala 
        515                 520                 525             


Ala Ile Ala Arg Gly Arg Lys Leu Ala Ser Glu Gly Phe Ile Asp Asn 
    530                 535                 540                 


Gly Ala Asn Arg Met Leu Met Arg Ile Trp Glu Glu Leu Asn Pro Ala 
545                 550                 555                 560 


Asn Pro Leu Asp Arg Arg Cys Pro Tyr Cys Ala Glu Pro Ile Gly Ile 
                565                 570                 575     


Arg Ala Leu Phe Ser Gly Glu Ala Asp Ile Asp His Ile Ile Pro Tyr 
            580                 585                 590         


Ser Arg Ser Leu Asp Asp Asn Val Gly Asn Lys Val Val Ala His Arg 
        595                 600                 605             


Ser Cys Asn Arg Ala Lys Gly Asn Lys Thr Pro Tyr Glu Lys Trp Gly 
    610                 615                 620                 


Pro His Ile Asp Pro Glu Arg Trp Glu Arg Ile Ser Asp Gln Val Ala 
625                 630                 635                 640 


Arg Leu His Lys Ser Lys Gln Trp Arg Phe Gly Pro Asp Ala Met Glu 
                645                 650                 655     


Arg Ile Asp Arg Asp Gly Gly Phe Leu Ala Arg Gln Leu Thr Asp Thr 
            660                 665                 670         


Gln Tyr Leu Ser Arg Val Ala Gly Lys Tyr Leu Arg Ser Leu Tyr Pro 
        675                 680                 685             


Thr Ala Gln Ser Gly Arg Val Asp Val Ile Pro Gly Arg Met Thr Ala 
    690                 695                 700                 


Met Leu Arg Arg Lys Trp Gly Leu Asn Ser Leu Leu Pro Asp His Asn 
705                 710                 715                 720 


Tyr Val Glu Asn Glu His Ser Asn Ala Pro Lys Asn Arg Leu Asp His 
                725                 730                 735     


Arg His His Ala Ile Asp Ala Ala Val Ala Ala Val Thr Thr Leu Ser 
            740                 745                 750         


Leu Met Gln Gln Ile Ala Asp Ala Ala Gly Arg Ala Glu Asp Lys Asn 
        755                 760                 765             


Leu Asp Arg Leu Phe Glu Asp Ile Pro Gln Pro Trp Pro Gly Phe Arg 
    770                 775                 780                 


Glu Glu Leu Gly Glu Ala Leu Arg Ser Thr Thr Val Ser His Lys Ala 
785                 790                 795                 800 


Asp His Gly Arg Lys Arg Arg Pro Ser Arg Asp Arg Asp Val Thr Ala 
                805                 810                 815     


Ala Arg Leu His Asn Asp Thr Ala Tyr Gly Leu Thr Gly Asp Val Ala 
            820                 825                 830         


Ala Asp Gly Lys Thr Pro Ile Val Val His Arg Val Pro Leu Ala Ser 
        835                 840                 845             


Leu Lys Pro Ala Asp Leu Ile Asp Pro Leu Arg Ile Pro Asp Pro Val 
    850                 855                 860                 


Leu Arg Asp Ala Leu Tyr Ala Ala Thr Arg Asp Arg Thr Gly Lys Glu 
865                 870                 875                 880 


Phe Glu Gln Ala Leu Thr Gln Phe Ala Arg His His Pro Leu Phe Lys 
                885                 890                 895     


Gly Ile Arg Arg Val Arg Val Arg Glu Pro Leu Asn Val Ile Pro Ile 
            900                 905                 910         


Arg Asp Ala Ala Gly Arg Ala Tyr Lys Ala Tyr Lys Gly Asp Ala Asn 
        915                 920                 925             


Ala Arg Phe Asp Val Trp Arg Leu Pro Asn Gly Lys Trp Val Ser Asp 
    930                 935                 940                 


Ile Val Ser Met Phe Asp Ala His Cys Met Ala Asp Lys Ser Asp Arg 
945                 950                 955                 960 


Arg Pro His Pro Ala Ala Lys Lys Val Leu Ser Leu Arg Gln Asn Asp 
                965                 970                 975     


Met Val Ala Ile Glu Arg Ser Gly Val Thr Gln Ile Ala Arg Val Val 
            980                 985                 990         


Lys Phe Ser Thr Asn Gly Ser Ile  Ala Leu Ala Ala Asp  Asn Glu Ala 
        995                 1000                 1005             


Gly Ala  Leu Lys Ala Arg Asp  Ala Ala Pro Asn Asp  Val Asp Pro 
    1010                 1015                 1020             


Phe Lys  Tyr Ile Phe Ser Ser  Gly Gly Gly Leu Lys  Asn Leu Lys 
    1025                 1030                 1035             


Ala Arg  Gln Val Arg Val Asp  Pro Leu Gly Arg Val  Phe Asp Pro 
    1040                 1045                 1050             


Gly Pro  Arg Glu 
    1055         


<210>  543
<211>  1058
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  543

Met Lys Asn Ser Tyr Ile Leu Gly Leu Asp Val Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile His Ser Asp Ser Lys Ser Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ala Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Asp Arg Val Lys Tyr Leu Leu Ala Gln Tyr His Leu Ile Ile Asp Asp 
65                  70                  75                  80  


Lys Ile Pro Lys Ser Thr Asn Pro Tyr Ala Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Ser Ala Leu Ser Lys Ser Glu Leu Val Ile Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Asn Val Asn Val Val Met Asp Glu Asn 
        115                 120                 125             


Glu Ser Thr Asn Glu Leu Ser Thr Lys Glu Gln Leu Lys Glu Asn Ala 
    130                 135                 140                 


Lys Gln Leu Glu Asn Arg Tyr Val Cys Glu Leu Gln Leu Asp Arg Leu 
145                 150                 155                 160 


Asn Glu Gln Tyr Lys Val Arg Gly Glu Arg Asn Arg Phe Lys Thr Glu 
                165                 170                 175     


Asp Phe Val Arg Glu Ala Arg Gln Ile Leu Thr Thr Gln Gln Lys Tyr 
            180                 185                 190         


His Asp Ile Asp Glu His Phe Ile Glu Gln Tyr Thr His Leu Leu Glu 
        195                 200                 205             


Thr Arg Arg Glu Tyr Tyr Glu Gly Pro Gly Lys Gly Ser Gln Tyr Gly 
    210                 215                 220                 


Trp Glu Gly Asp Leu Lys Gln Trp Tyr Glu Met Leu Met Gly Arg Cys 
225                 230                 235                 240 


Thr Tyr Phe Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Thr Ala 
                245                 250                 255     


Asp Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Ala Arg 
            260                 265                 270         


Asp Glu Asn Glu Lys Leu Glu Tyr Phe Glu Lys Tyr His Ile Ile Glu 
        275                 280                 285             


Asn Val Phe Lys Gln Lys Lys Ser Pro Thr Leu Lys Gln Ile Ala Lys 
    290                 295                 300                 


Glu Ile Asp Val Lys Glu Ser Asp Ile Lys Gly Tyr Arg Ile Thr Lys 
305                 310                 315                 320 


Asn Gly Lys Pro Gln Phe Thr Ser Phe Lys Leu Tyr His Asp Leu Asn 
                325                 330                 335     


Lys Ile Phe Leu Asp Lys Lys Asn Leu Glu Asn Ile Glu Leu Leu Asp 
            340                 345                 350         


Glu Ile Ala Phe Ile Leu Thr Leu Tyr Gln Asp Pro Leu Ser Ile Lys 
        355                 360                 365             


Glu Ala Leu Asp Gln Leu Gln Asp Leu Leu Thr Glu Ser Glu Lys Glu 
    370                 375                 380                 


Ala Ile Ala His Leu Asn Gly Tyr Thr Gly Thr His Arg Leu Ser Leu 
385                 390                 395                 400 


Lys Cys Leu His Leu Leu Ile Asp Glu Met Trp Gln Thr Ser Arg Asn 
                405                 410                 415     


Gln Met Glu Val Phe Thr Ala Leu Asn Leu Lys Pro Gln Lys Tyr Gln 
            420                 425                 430         


Leu Ser Lys Gln Lys Gln Ile Pro Lys Lys Met Val Glu Glu Phe Ile 
        435                 440                 445             


Leu Ser Pro Val Val Lys Arg Ser Phe Ile Gln Ser Ile Glu Ile Val 
    450                 455                 460                 


Asn Thr Ile Ile Lys Lys Tyr Gly Leu Pro Glu Glu Ile Ile Ile Glu 
465                 470                 475                 480 


Leu Ala Arg Glu Lys Asn Ser Glu Asp Arg Arg Lys Phe Leu Asn Arg 
                485                 490                 495     


Ile Gln Lys Glu Asn Glu Asn Thr Arg Lys Gln Val Glu Glu Val Ile 
            500                 505                 510         


Arg Glu Tyr Gly Asn Arg Asn Ala Lys Gly Leu Val Glu Lys Ile Lys 
        515                 520                 525             


Leu His Gln Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Gln Asn Ile 
    530                 535                 540                 


Pro Leu Asp Asp Leu Leu Arg Asn Pro Ser His Tyr Glu Ile Asp His 
545                 550                 555                 560 


Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val 
                565                 570                 575     


Leu Val Lys Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Tyr 
            580                 585                 590         


Gln Tyr Leu Asn Ser Asn Glu Ser Val Leu Ser Tyr Val Gln Phe Lys 
        595                 600                 605             


Gln His Ile Leu Asn Leu Ser Lys Ser Lys Glu Arg Ile Ser Lys Lys 
    610                 615                 620                 


Lys Arg Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val 
625                 630                 635                 640 


Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr 
                645                 650                 655     


Arg Glu Leu Val Gln Leu Leu Lys Ser Tyr Phe Ser Ala Asn Asp Leu 
            660                 665                 670         


Asp Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asn Tyr Leu Arg 
        675                 680                 685             


Lys Ala Trp Lys Phe Asp Lys Ser Arg Asn Lys Gly Phe Lys His His 
    690                 695                 700                 


Ala Glu Asp Ala Leu Ile Ile Ala Asn Val Ser Tyr Leu Phe Lys Gln 
705                 710                 715                 720 


Ser Lys Glu Leu Lys Glu Ala Asn Ala Ile Leu Glu Asn Asn Asn Leu 
                725                 730                 735     


Ser Leu Asn Lys Asn Lys Asp Ser Lys Asn Leu Leu Thr Glu Gln Asp 
            740                 745                 750         


Tyr Arg Lys Met Phe Glu Ile Pro Lys Gln Val Gln Asp Ile Lys Asp 
        755                 760                 765             


Tyr Lys Asp Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg 
    770                 775                 780                 


Gln Leu Ile Asn Asp Ser Leu Tyr Ser Thr Arg Glu Val Asn Glu Asn 
785                 790                 795                 800 


His Tyr Ile Val Gln Thr Val Lys Asp Ile Tyr Ala Lys Asp Asn Thr 
                805                 810                 815     


Lys Ile Lys Asp Leu Phe Leu Lys Thr Pro Glu Lys Leu Leu Met Tyr 
            820                 825                 830         


Gln His Asp Pro Gln Thr Phe Glu Lys Leu Met Leu Val Ile Lys Gln 
        835                 840                 845             


Tyr Gly Glu Glu Lys Asn Pro Phe Ala Lys Tyr Tyr Glu Glu Asn Gly 
    850                 855                 860                 


Glu Tyr Leu Thr Lys Tyr Ala Lys Asn Glu Lys Gly Pro Thr Ile Lys 
865                 870                 875                 880 


Arg Leu Lys Tyr Ile Gly Lys Lys Val Gly Thr His Leu Asp Ile Ser 
                885                 890                 895     


Thr Asn Phe Ser Gly Ala Lys Arg Lys Val Val Gln Leu Ser Ile Lys 
            900                 905                 910         


Pro Phe Arg Phe Asp Val Phe His Ser Lys Ala Gly Tyr His Met Leu 
        915                 920                 925             


Thr Leu Thr Tyr Met Asp Ile Lys Lys Lys Glu Lys His Tyr Gln Ile 
    930                 935                 940                 


Leu Lys Asn Glu Tyr Gln Ala Leu Lys Glu Arg Tyr Lys Val Pro Ser 
945                 950                 955                 960 


Asp Ala Gln Phe Ile Gly Ser Phe Tyr Tyr Asn Asp Leu Ile Ser Ile 
                965                 970                 975     


Asn Asp Glu Leu Phe Arg Val Ile Gly Val Asn Asn Ser Thr Arg Asn 
            980                 985                 990         


Val Val Glu Leu Asn Met Val Asp  Ile Asn Tyr Lys Asp  Tyr Cys Asp 
        995                 1000                 1005             


Leu Met  Gly Ile Lys Lys Thr  Pro Arg Ile Phe Lys  Thr Ile Thr 
    1010                 1015                 1020             


Lys Lys  Thr Gly Lys Ile Glu  Lys Tyr Ser Thr Asp  Ile Leu Gly 
    1025                 1030                 1035             


Asn Gln  Phe Lys Val Lys Pro  Ser Lys Ser Pro Gln  Leu Ile Phe 
    1040                 1045                 1050             


Lys Arg  Gly Val Leu 
    1055             


<210>  544
<211>  1059
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  544

Met Arg Arg Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly Trp Cys 
1               5                   10                  15      


Leu Thr Gln Gly Pro Gly Asp Glu Ala Asp Gly Ile Val Asp Ile Gly 
            20                  25                  30          


Val Arg Ile Phe Ser Asp Gly Arg Asp Pro Gln Ser Gly Thr Ser Leu 
        35                  40                  45              


Ala Val Asp Arg Arg Asp Ala Arg Ala Ala Arg Arg Arg Arg Asp Arg 
    50                  55                  60                  


Tyr Ile Gly Arg Arg Ser Ala Phe Leu Asp Thr Leu Val Ala His Gly 
65                  70                  75                  80  


Leu Met Pro Ala Asp Ala Asp Glu Ala Lys Leu Val Ala Ala Leu Asp 
                85                  90                  95      


Pro Tyr Thr Leu Arg Ala Arg Ala Leu Glu Ala Pro Leu Thr Pro His 
            100                 105                 110         


Glu Ile Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ala Glu Arg Lys Thr Asn Asp Ala Asp Asp Gly Lys 
    130                 135                 140                 


Ile Ala Thr Ala Ala Tyr Ala Leu Asp Arg Ala Met Leu Asp Gly Gly 
145                 150                 155                 160 


Phe Asp Thr Leu Gly Gln Phe Leu Asn Ala Gln Glu Thr Lys Arg Val 
                165                 170                 175     


Arg Met Asp Gly Glu Ala Gln Gly Tyr Asp Phe Tyr Pro Gln Arg Arg 
            180                 185                 190         


His Val Glu Tyr Glu Phe Asp Arg Ile Trp Thr Glu Gln Ala Lys His 
        195                 200                 205             


His Pro Ala Leu Leu Thr Glu Thr Ala Arg Tyr Ala Leu His Arg Ile 
    210                 215                 220                 


Leu Phe Tyr Gln Arg Pro Leu Lys Ala Gln Val Val Gly Lys Cys Thr 
225                 230                 235                 240 


Phe Val His Ser Glu Asp Arg Leu Ala Lys Ala His Pro Leu Phe Gln 
                245                 250                 255     


Gln Arg Arg Leu Tyr Glu Glu Val Asn Gln Leu Glu Val Thr Thr Pro 
            260                 265                 270         


Gly Glu Ala Asn Arg Lys Leu Thr Leu Asp Glu Arg Asp Arg Leu Val 
        275                 280                 285             


Arg Glu Leu Ser His Lys Lys Ala Ile Ala Phe Thr Ser Leu Ala Ala 
    290                 295                 300                 


Lys Ile Lys Leu Glu Pro Gly Gln Ser Phe Asn Lys Ala Ser Glu Thr 
305                 310                 315                 320 


Arg Thr Gln Leu Ala Gly Asp Glu Val Arg Ala Val Leu Gly Asp Lys 
                325                 330                 335     


Lys Arg Phe Gly Pro Ala Trp Glu His Phe Asp Arg Asp Arg Gln Trp 
            340                 345                 350         


Thr Ile Val Glu Arg Leu Leu Glu Glu Glu Asp Pro Asp Ala Leu His 
        355                 360                 365             


Ala Phe Leu Thr Gln Asp Cys Gly Leu Asp Asp Glu His Ala Ala Ala 
    370                 375                 380                 


Thr Ala Lys Thr Ser Leu Pro Glu Gly Tyr Gly Arg Leu Gly Ala Thr 
385                 390                 395                 400 


Ala Thr Ala Arg Ile Leu Ala Glu Leu Gln Ala Glu Val Ile Thr Tyr 
                405                 410                 415     


Asp Glu Ala Val Arg Arg Cys Gly Trp His His Ser Asp His Arg Thr 
            420                 425                 430         


Gly Glu Ile Leu Pro Gln Leu Pro Tyr Tyr Gly Glu Leu Leu Thr Arg 
        435                 440                 445             


Asp Ile Pro Pro Gly Thr Gln Asp Pro Asn Asp Pro Pro Glu Lys Arg 
    450                 455                 460                 


Trp Gly Arg Ile Thr Asn Pro Thr Val His Ile Gly Leu Asn Gln Val 
465                 470                 475                 480 


Arg Gln Leu Val Asn Ala Ile Val Ala Val His Gly Arg Pro Asp Gln 
                485                 490                 495     


Ile Val Val Glu Leu Ala Arg Glu Leu Lys Leu Asn Glu Lys Asp Lys 
            500                 505                 510         


Glu Glu His Asn Arg Arg Ile Arg Arg Asp Thr Ala Ala Ala Ile Lys 
        515                 520                 525             


Arg Ser Glu Lys Leu Thr Gln Glu Gly Phe Pro Asp Thr Gly Ala Asn 
    530                 535                 540                 


Arg Met Leu Leu Arg Leu Trp Glu Asp Leu Asn Pro Ala Asn Pro Leu 
545                 550                 555                 560 


Asp Arg Arg Cys Pro Tyr Cys Gly Ala Pro Ile Gly Met Arg Ala Leu 
                565                 570                 575     


Phe Ala Gly Glu Ala Asp Val Asp His Ile Ile Pro Tyr Ser Arg Ser 
            580                 585                 590         


Leu Asp Asp Ser Ala Gly Asn Lys Val Val Ala His Arg Ser Cys Asn 
        595                 600                 605             


Arg Ala Lys Gly Asn Arg Thr Pro Tyr Glu Lys Trp Ser Asp Asp Pro 
    610                 615                 620                 


Asp Arg Trp Asp Ala Ile Thr Ala Gln Val Ala Arg Leu His Arg Ser 
625                 630                 635                 640 


Lys Gln Trp Arg Phe Gly Pro Asp Ala Met Ala Arg Val Glu Lys Asp 
                645                 650                 655     


Gly Gly Phe Leu Ser Arg Gln Leu Thr Asp Thr Gln Tyr Leu Ser Arg 
            660                 665                 670         


Leu Ala Gly Arg Tyr Leu Ser Ser Leu Tyr Pro Thr Lys Glu Glu Gly 
        675                 680                 685             


Ser Val Tyr Val Ile Pro Gly Arg Met Thr Ala Met Leu Arg Arg Leu 
    690                 695                 700                 


Trp Gly Leu Asn Asp Ile Leu Ser Asp His Asn Ile Val Glu Asn Lys 
705                 710                 715                 720 


His Ser Asn Ala Pro Lys Asn Arg Leu Asp His Arg His His Ala Ile 
                725                 730                 735     


Asp Ala Ala Val Val Ala Val Ser Thr Arg Ser Leu Met Gln Arg Ile 
            740                 745                 750         


Ala Arg Thr Ala Gly Gln Ala Glu Asp Arg Asn Leu Asp Arg Leu Phe 
        755                 760                 765             


Glu Gly Leu Asp Pro Pro Trp Pro Gly Phe Arg Asp Gln Leu Arg Asp 
    770                 775                 780                 


Arg Leu Leu Ala Val Thr Val Ser His Lys Ala Asp His Gly Arg Thr 
785                 790                 795                 800 


Gly Thr Pro Val Pro Gly Lys Asp Ala Thr Ala Gly Arg Leu His Asn 
                805                 810                 815     


Asp Thr Ala Tyr Gly Leu Thr Gly Glu Thr Ser Ala Lys Gly Leu Pro 
            820                 825                 830         


Ile Val Val His Arg Val Pro Leu Thr Ser Leu Lys Pro Ala Asp Ile 
        835                 840                 845             


Thr Asp Pro Asp Arg Ile Pro Asp Pro Val Leu Arg Asp Ala Ile Glu 
    850                 855                 860                 


Arg Ala Thr Ala Gly Lys Met Gly Lys Asp Phe Thr Asp Ala Leu Ala 
865                 870                 875                 880 


Lys Phe Gly Arg Ser Gly Ala Gln Glu Thr Asp Arg Lys Gly Glu Arg 
                885                 890                 895     


Leu Phe Arg Gly Thr Arg Arg Val Arg Val Arg Glu Pro Leu Gly Val 
            900                 905                 910         


Ile Pro Ile Ala Asp Ala Ser Gly Arg Val Tyr Lys Ala Tyr Lys Gly 
        915                 920                 925             


Asp Ser Asn Ala Arg Phe Asp Val Trp Arg Leu Pro Asp Gly Lys Trp 
    930                 935                 940                 


Val Ser Asp Ile Val Ser Met Phe Asp Ala His Arg Pro Asp Gln Gly 
945                 950                 955                 960 


Asp Arg Arg Pro His Pro Ala Ala Lys Lys Val Leu Ser Leu Arg Gln 
                965                 970                 975     


Asn Asp Met Ile Ala Ile Glu Arg Asp Gly Ala Pro Arg Glu Ile Val 
            980                 985                 990         


Arg Val Val Lys Phe Ser Thr Ile  Gly Ser Val Gln Leu  Ala Ala Thr 
        995                 1000                 1005             


Thr Glu  Ala Gly Pro Leu Lys  Ala Arg Asp Ala Ser  Pro Pro Asp 
    1010                 1015                 1020             


Val Asp  Pro Phe Arg Tyr Ile  Asn Ser Ser Ala Gly  Gly Leu Lys 
    1025                 1030                 1035             


Lys Ile  Lys Ala Arg Gln Val  Arg Ile Asp Pro Leu  Gly Arg Ile 
    1040                 1045                 1050             


Phe Asp  Pro Gly Pro Arg 
    1055                 


<210>  545
<211>  1059
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  545

Met Ile Glu Arg Ile Leu Gly Val Asp Leu Gly Ile Ser Ser Leu Gly 
1               5                   10                  15      


Trp Ala Ile Val Glu Tyr Asp Lys Asp Asp Glu Ala Ala Asn Arg Ile 
            20                  25                  30          


Ile Asp Cys Gly Val Arg Leu Phe Thr Ala Ala Glu Thr Pro Lys Lys 
        35                  40                  45              


Lys Glu Ser Pro Asn Lys Ala Arg Arg Glu Ala Arg Gly Ile Arg Arg 
    50                  55                  60                  


Val Leu Asn Arg Arg Arg Val Arg Met Asn Met Ile Lys Lys Leu Phe 
65                  70                  75                  80  


Leu Arg Ala Gly Leu Ile Gln Asp Val Asp Leu Asp Gly Glu Gly Gly 
                85                  90                  95      


Met Phe Tyr Ser Lys Ala Asn Arg Ala Asp Val Trp Glu Leu Arg His 
            100                 105                 110         


Asp Gly Leu Tyr Arg Leu Leu Lys Gly Asp Glu Leu Ala Arg Val Leu 
        115                 120                 125             


Ile His Ile Ala Lys His Arg Gly Tyr Lys Phe Ile Gly Asp Asp Glu 
    130                 135                 140                 


Ala Asp Glu Glu Ser Gly Lys Val Lys Lys Ala Gly Val Val Leu Arg 
145                 150                 155                 160 


Gln Asn Phe Glu Ala Ala Gly Cys Arg Thr Val Gly Glu Trp Leu Trp 
                165                 170                 175     


Arg Glu Arg Gly Ala Asn Gly Lys Lys Arg Asn Lys His Gly Asp Tyr 
            180                 185                 190         


Glu Ile Ser Ile His Arg Asp Leu Leu Val Glu Glu Val Glu Ala Ile 
        195                 200                 205             


Phe Val Ala Gln Gln Glu Met Arg Ser Thr Ile Ala Thr Asp Ala Leu 
    210                 215                 220                 


Lys Ala Ala Tyr Arg Glu Ile Ala Phe Phe Val Arg Pro Met Gln Arg 
225                 230                 235                 240 


Ile Glu Lys Met Val Gly His Cys Thr Tyr Phe Pro Glu Glu Arg Arg 
                245                 250                 255     


Ala Pro Lys Ser Ala Pro Thr Ala Glu Lys Phe Ile Ala Ile Ser Lys 
            260                 265                 270         


Phe Phe Ser Thr Val Ile Ile Asp Asn Glu Gly Trp Glu Gln Lys Ile 
        275                 280                 285             


Ile Glu Arg Lys Thr Leu Glu Glu Leu Leu Asp Phe Ala Val Ser Arg 
    290                 295                 300                 


Glu Lys Val Glu Phe Arg His Leu Arg Lys Phe Leu Asp Leu Ser Asp 
305                 310                 315                 320 


Asn Glu Ile Phe Lys Gly Leu His Tyr Lys Gly Lys Pro Lys Thr Ala 
                325                 330                 335     


Lys Lys Arg Glu Ala Thr Leu Phe Asp Pro Asn Glu Pro Thr Glu Leu 
            340                 345                 350         


Glu Phe Asp Lys Val Glu Ala Glu Lys Lys Ala Trp Ile Ser Leu Arg 
        355                 360                 365             


Gly Ala Ala Lys Leu Arg Glu Ala Leu Gly Asn Glu Phe Tyr Gly Arg 
    370                 375                 380                 


Phe Val Ala Leu Gly Lys His Ala Asp Glu Ala Thr Lys Ile Leu Thr 
385                 390                 395                 400 


Tyr Tyr Lys Asp Glu Gly Gln Lys Arg Arg Glu Leu Thr Lys Leu Pro 
                405                 410                 415     


Leu Glu Ala Glu Met Val Glu Arg Leu Val Lys Ile Gly Phe Ser Asp 
            420                 425                 430         


Phe Leu Lys Leu Ser Leu Lys Ala Ile Arg Asp Ile Leu Pro Ala Met 
        435                 440                 445             


Glu Ser Gly Ala Arg Tyr Asp Glu Ala Val Leu Met Leu Gly Val Pro 
    450                 455                 460                 


His Lys Glu Lys Ser Ala Ile Leu Pro Pro Leu Asn Lys Thr Asp Ile 
465                 470                 475                 480 


Asp Ile Leu Asn Pro Thr Val Ile Arg Ala Phe Ala Gln Phe Arg Lys 
                485                 490                 495     


Val Ala Asn Ala Leu Val Arg Lys Tyr Gly Ala Phe Asp Arg Val His 
            500                 505                 510         


Phe Glu Leu Ala Arg Glu Ile Asn Thr Lys Gly Glu Ile Glu Asp Ile 
        515                 520                 525             


Lys Glu Ser Gln Arg Lys Asn Glu Lys Glu Arg Lys Glu Ala Ala Asp 
    530                 535                 540                 


Trp Ile Ala Glu Thr Ser Phe Gln Val Pro Leu Thr Arg Lys Asn Ile 
545                 550                 555                 560 


Leu Lys Lys Arg Leu Tyr Ile Gln Gln Asp Gly Arg Cys Ala Tyr Thr 
                565                 570                 575     


Gly Asp Val Ile Glu Leu Glu Arg Leu Phe Asp Glu Gly Tyr Cys Glu 
            580                 585                 590         


Ile Asp His Ile Leu Pro Arg Ser Arg Ser Ala Asp Asp Ser Phe Ala 
        595                 600                 605             


Asn Lys Val Leu Cys Leu Ala Arg Ala Asn Gln Gln Lys Thr Asp Arg 
    610                 615                 620                 


Thr Pro Tyr Glu Trp Phe Gly His Asp Ala Ala Arg Trp Asn Ala Phe 
625                 630                 635                 640 


Glu Thr Arg Thr Ser Ala Pro Ser Asn Arg Val Arg Thr Gly Lys Gly 
                645                 650                 655     


Lys Ile Asp Arg Leu Leu Lys Lys Asn Phe Asp Glu Asn Ser Glu Met 
            660                 665                 670         


Ala Phe Lys Asp Arg Asn Leu Asn Asp Thr Arg Tyr Met Ala Arg Ala 
        675                 680                 685             


Ile Lys Thr Tyr Cys Glu Gln Tyr Trp Val Phe Lys Asn Ser His Thr 
    690                 695                 700                 


Lys Ala Pro Val Gln Val Arg Ser Gly Lys Leu Thr Ser Val Leu Arg 
705                 710                 715                 720 


Tyr Gln Trp Gly Leu Glu Ser Lys Asp Arg Glu Ser His Thr His His 
                725                 730                 735     


Ala Val Asp Ala Ile Ile Ile Ala Phe Ser Thr Gln Gly Met Val Gln 
            740                 745                 750         


Lys Leu Ser Glu Tyr Tyr Arg Phe Lys Glu Thr His Arg Glu Lys Glu 
        755                 760                 765             


Arg Pro Lys Leu Ala Val Pro Leu Ala Asn Phe Arg Asp Ala Val Glu 
    770                 775                 780                 


Glu Ala Thr Arg Ile Glu Asn Thr Glu Thr Val Lys Glu Gly Val Glu 
785                 790                 795                 800 


Val Lys Arg Leu Leu Ile Ser Arg Pro Pro Arg Ala Arg Val Thr Gly 
                805                 810                 815     


Gln Ala His Glu Gln Thr Ala Lys Pro Tyr Pro Arg Ile Lys Gln Val 
            820                 825                 830         


Lys Asn Lys Lys Lys Trp Arg Leu Ala Pro Ile Asp Glu Glu Lys Phe 
        835                 840                 845             


Glu Ser Phe Lys Ala Asp Arg Val Ala Ser Ala Asn Gln Lys Asn Phe 
    850                 855                 860                 


Tyr Glu Thr Ser Thr Ile Pro Arg Val Asp Val Tyr His Lys Lys Gly 
865                 870                 875                 880 


Lys Phe His Leu Val Pro Ile Tyr Leu His Glu Met Val Leu Asn Glu 
                885                 890                 895     


Leu Pro Asn Leu Ser Leu Gly Thr Asn Pro Glu Ala Met Asp Glu Asn 
            900                 905                 910         


Phe Phe Lys Phe Ser Ile Phe Lys Asp Asp Leu Ile Ser Ile Gln Thr 
        915                 920                 925             


Gln Gly Thr Pro Lys Lys Pro Ala Lys Ile Ile Met Gly Tyr Phe Lys 
    930                 935                 940                 


Asn Met His Gly Ala Asn Met Val Leu Ser Ser Ile Asn Asn Ser Pro 
945                 950                 955                 960 


Cys Glu Gly Phe Thr Cys Thr Pro Val Ser Met Asp Lys Lys His Lys 
                965                 970                 975     


Asp Lys Cys Lys Leu Cys Pro Glu Glu Asn Arg Ile Ala Gly Arg Cys 
            980                 985                 990         


Leu Gln Gly Phe Leu Asp Tyr Trp  Ser Gln Glu Gly Leu  Arg Pro Pro 
        995                 1000                 1005             


Arg Lys  Glu Phe Glu Cys Asp  Gln Gly Val Lys Phe  Ala Leu Asp 
    1010                 1015                 1020             


Val Lys  Lys Tyr Gln Ile Asp  Pro Leu Gly Tyr Tyr  Tyr Glu Val 
    1025                 1030                 1035             


Lys Gln  Glu Lys Arg Leu Gly  Thr Ile Pro Gln Met  Arg Ser Ala 
    1040                 1045                 1050             


Lys Lys  Leu Val Lys Lys 
    1055                 


<210>  546
<211>  1059
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  546

Met Ser Lys Arg Leu Arg Tyr Arg Leu Ala Leu Asp Leu Gly Thr Asn 
1               5                   10                  15      


Ser Ile Gly Phe Cys Leu Leu Asp Leu Asn Gly Asp Gly Arg Pro Cys 
            20                  25                  30          


Gly Val Arg Arg Met Gly Val Arg Ile Phe Pro Asp Phe Ser Lys Leu 
        35                  40                  45              


Gln Gln Ser Leu Ala Ala Asp Arg Arg Leu Ala Arg Ser Met Arg Arg 
    50                  55                  60                  


Arg Arg Asp Arg Tyr Leu Arg Arg Arg Lys Ala Leu Met Asn Ala Leu 
65                  70                  75                  80  


Val Ala Ala Ala Leu Met Pro Ala Asp Pro Lys Ala Arg Lys Ala Leu 
                85                  90                  95      


Glu Ala Leu Asp Pro Tyr Glu Leu Arg Ala Ala Ala Val Ala Gly Pro 
            100                 105                 110         


Leu Pro Ala His Gln Leu Gly Arg Ala Leu Phe His Leu Asn Gln Arg 
        115                 120                 125             


Arg Gly Phe Lys Ser Asn Arg Lys Ala Ala Gly Asp Glu Asn Glu Ala 
    130                 135                 140                 


Gly Lys Val Lys Ser Gly Ile Ser Ala Leu Lys Ala Glu Leu Ala Ala 
145                 150                 155                 160 


Ser Gly Ala Gly Thr Leu Gly Gln Trp Leu Ala Ala Arg His Ala Asn 
                165                 170                 175     


Arg Gln Pro Val Arg Ala Arg Leu Thr Gly Thr Gly Ala Lys Ala Thr 
            180                 185                 190         


Tyr Ser Phe Tyr Pro Glu Arg Ala Leu Val His Glu Glu Phe Gln Ala 
        195                 200                 205             


Ile Arg Ala Ala Gln Ala Pro His His Pro Gly Val Thr Ala Glu Gln 
    210                 215                 220                 


Trp Asp Arg Ile Glu Ser Val Ile Phe His Gln Arg Pro Leu Lys Pro 
225                 230                 235                 240 


Val Lys Pro Gly Lys Cys Thr Leu Tyr Pro Glu Glu Glu Arg Ala Pro 
                245                 250                 255     


Trp Ala Leu Pro Ile Ala Gln Arg Phe Arg Ile Leu His Glu Leu His 
            260                 265                 270         


Asn Leu Arg Ile Val Arg Pro Gly Gln Gly Glu Glu Gly Leu Ser Pro 
        275                 280                 285             


Ala Asp Phe Asp Arg Phe Phe Asp Arg Leu Cys Arg Lys Gly Asp Leu 
    290                 295                 300                 


Thr Phe Lys Thr Leu Ala Lys Glu Leu Lys Leu Pro Pro Asp Ala Arg 
305                 310                 315                 320 


Ile Asn Leu Glu Asp Asp Lys Arg Asp Arg Leu Lys Gly Asp Ala Thr 
                325                 330                 335     


Ala Asp Ala Leu Ser Ala Glu Lys Gly Lys Arg Arg Arg Ile Gly Ala 
            340                 345                 350         


Ala Trp His Ser Phe Pro Ala Asp Arg Gln Arg Glu Ile Val Gly Arg 
        355                 360                 365             


Leu Ile Asp Ala Glu Asp Glu Gly Glu Leu Ala Ala Trp Leu Val Glu 
    370                 375                 380                 


Glu Leu Gly Leu Pro Trp Glu Val Ala Ile Leu Thr Ala Glu Arg Pro 
385                 390                 395                 400 


Gly Leu Pro Asp Gly Tyr Cys Arg Leu Ser Glu Lys Ala Leu Glu Gln 
                405                 410                 415     


Val Val Pro Leu Met His Glu Glu His Leu Pro Tyr Asp Glu Ala Val 
            420                 425                 430         


Arg Ala Leu Gly Leu His His Ser Asp His Arg Thr Gly Glu Phe Leu 
        435                 440                 445             


Asp Glu Leu Pro Tyr Tyr Gly Leu Pro Leu Glu Arg Phe Ile Ala Phe 
    450                 455                 460                 


Gly Thr Gly Glu Ala Asp Asp Pro Pro Glu Leu Arg Ile Gly Arg Phe 
465                 470                 475                 480 


Pro Asn Pro Thr Val His Val Ala Leu Asn Glu Leu Arg Arg Val Val 
                485                 490                 495     


Asn Arg Ile Ile Arg Arg Trp Gly Lys Pro Glu Glu Ile Val Val Glu 
            500                 505                 510         


Leu Ala Arg Asp Leu Lys Gln Ser Arg Ala Asp Arg Asp Lys Thr Gln 
        515                 520                 525             


Arg Glu Gln Ala Asp Asn Gln Lys Lys Asn Trp Thr Arg Thr Gln Gln 
    530                 535                 540                 


Leu Arg Glu Leu Gly Met Glu Pro Thr Gly Glu Ala Leu Leu Arg Leu 
545                 550                 555                 560 


Arg Leu Trp Glu Glu Leu Pro Arg Ile Gly Ser Ala His Val Cys Val 
                565                 570                 575     


Tyr Thr Gly Gln Pro Ile Ser Met Thr Gln Leu Leu Gln Gly Asp Val 
            580                 585                 590         


Asp Val Asp His Ile Leu Pro Phe Ser Arg Thr Leu Ala Asn Gly Ala 
        595                 600                 605             


Asp Asn Lys Ile Val Cys His Arg Arg Ala Asn Arg Ile Lys Arg Asn 
    610                 615                 620                 


Arg Thr Pro Trp Glu Ala Phe Gly Val Val Ser Ala Asp Gly Tyr Asp 
625                 630                 635                 640 


Trp Ala Ala Ile Ala Glu Arg Ala Glu Ser Leu Pro Lys Asn Lys Arg 
                645                 650                 655     


Trp Arg Phe Leu Pro Asn Ala Met Glu Arg Phe Glu Thr Glu Arg Gly 
            660                 665                 670         


Phe Ile Asp Arg Gln Leu Val Glu Thr Arg His Leu Ser Arg Val Ala 
        675                 680                 685             


Arg Glu Tyr Leu Gly Cys Ile Val Pro Leu Glu Lys Val Arg Ala Ala 
    690                 695                 700                 


Pro Gly Arg Leu Thr Ala Met Leu Arg Gly Arg Trp Gly Leu Asn Ser 
705                 710                 715                 720 


Ile Leu Ser Asp Ala Asn Leu Lys Asn Arg Ser Asp His Arg His His 
                725                 730                 735     


Ala Val Asp Ala Ala Val Leu Gly Val Ile Asp Gln Arg Leu Val Gln 
            740                 745                 750         


Arg Val Ala Asp Ala Ala Lys Arg Ala Glu Glu Lys Glu Leu Glu Lys 
        755                 760                 765             


Leu Phe Asp Gly Leu Pro Asp Pro Phe Glu Gly Leu Pro Gly Pro Ser 
    770                 775                 780                 


Phe Arg Asp Gln Val Gln Glu Arg Ala Gln Gly Ile Ile Val Ser His 
785                 790                 795                 800 


Lys Pro Glu His Gly Trp Gln Gly Arg Met His Asn Asp Thr Ala Tyr 
                805                 810                 815     


Gly Pro Val Glu Arg Leu Pro Asp Gly Thr His Arg Val Val Thr Arg 
            820                 825                 830         


Lys Pro Leu Gly Ser Leu Ala Glu Lys Asp Phe Gly Asn Ile Arg Asp 
        835                 840                 845             


Arg Ala Leu Ala Ala Ala Leu Glu Arg His Val Ala Glu Gln Ala Ala 
    850                 855                 860                 


Gln Gly Arg Ser Leu Gly Glu Ala Leu Ser Cys Phe Thr Trp Thr Gly 
865                 870                 875                 880 


Gly Glu Val Arg Arg Val Arg Met Leu Asp Lys Leu Gly Ser Pro Val 
                885                 890                 895     


Ala Val Gln Asp Asp Asp Gly His Thr Phe Lys Leu Tyr Lys Gly Asp 
            900                 905                 910         


Ser Asn Ala Trp Tyr Glu Ile Leu Arg Asp Gly Arg Gly Arg Trp Thr 
        915                 920                 925             


Gly Arg Ile Val Ser Thr Tyr Glu Ala Tyr Arg Leu His Ala Glu Trp 
    930                 935                 940                 


Leu Ala Glu Arg Gly Asp Gly Gly Arg Ser Asp Ala Ala Phe Pro Asp 
945                 950                 955                 960 


Trp Ala Ala Arg Arg Leu Cys Ala Gly Ile Gly Lys Pro Leu Phe Arg 
                965                 970                 975     


Leu Phe Lys Gly Asp Met Leu Ala Phe Gly Thr Ala Pro Asn Arg Glu 
            980                 985                 990         


Ile Met Lys Val Val Lys Phe Ser  Glu Gly Arg Ile Val  Phe Ala Gly 
        995                 1000                 1005             


His Arg  Glu Ala Gly Asn Leu  Lys Glu Arg Asp Ala  Asp Lys Asp 
    1010                 1015                 1020             


Asp Pro  Phe Lys Tyr Arg Thr  Cys Ser Pro Glu Ala  Leu Arg Lys 
    1025                 1030                 1035             


Ala Gln  Ala Arg Pro Val Gly  Val Asp Pro Ala Gly  Phe Val His 
    1040                 1045                 1050             


Asp Arg  Gly Pro Leu Glu 
    1055                 


<210>  547
<211>  1059
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  547

Met Gly Ile Arg Phe Ala Phe Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Trp Arg Thr Gly Pro Gly Val Phe Gly Glu Asp Thr Ala Ala 
            20                  25                  30          


Ser Leu Asp Gly Ser Gly Val Leu Ile Phe Lys Asp Gly Arg Asn Pro 
        35                  40                  45              


Lys Asp Gly Gln Ser Leu Ala Thr Met Arg Arg Val Pro Arg Gln Ser 
    50                  55                  60                  


Arg Lys Arg Arg Asp Arg Phe Val Leu Arg Arg Arg Asp Leu Leu Ala 
65                  70                  75                  80  


Ala Leu Arg Lys Ala Gly Leu Phe Pro Val Asp Val Glu Glu Gly Arg 
                85                  90                  95      


Arg Leu Ala Ala Thr Asp Pro Tyr His Leu Arg Ala Lys Ala Leu Asp 
            100                 105                 110         


Glu Ser Leu Thr Pro His Glu Met Gly Arg Val Ile Phe His Leu Asn 
        115                 120                 125             


Gln Arg Arg Gly Phe Arg Ser Asn Arg Lys Ala Asp Arg Gln Asp Arg 
    130                 135                 140                 


Glu Lys Gly Lys Ile Ala Glu Gly Ser Lys Arg Leu Ala Glu Thr Leu 
145                 150                 155                 160 


Ala Ala Thr Asn Cys Arg Thr Leu Gly Glu Phe Leu Trp Ser Arg His 
                165                 170                 175     


Arg Gly Thr Pro Arg Thr Arg Ser Pro Thr Arg Ile Arg Met Glu Gly 
            180                 185                 190         


Glu Gly Ala Lys Ala Leu Tyr Ala Phe Tyr Pro Thr Arg Glu Met Val 
        195                 200                 205             


Arg Ala Glu Phe Glu Arg Leu Trp Thr Ala Gln Ser Arg Phe Ala Pro 
    210                 215                 220                 


Asp Leu Leu Thr Pro Glu Arg His Glu Glu Ile Ala Gly Ile Leu Phe 
225                 230                 235                 240 


Arg Gln Arg Asp Leu Ala Pro Pro Lys Ile Gly Cys Cys Thr Phe Glu 
                245                 250                 255     


Pro Ser Glu Arg Arg Leu Pro Arg Ala Leu Pro Ser Val Glu Ala Arg 
            260                 265                 270         


Gly Ile Tyr Glu Arg Leu Ala His Leu Arg Ile Thr Thr Gly Pro Val 
        275                 280                 285             


Ser Asp Arg Gly Leu Thr Arg Pro Glu Arg Asp Val Leu Ala Ser Ala 
    290                 295                 300                 


Leu Leu Ala Gly Lys Ser Leu Thr Phe Lys Ala Val Arg Lys Thr Leu 
305                 310                 315                 320 


Lys Ile Leu Pro His Ala Leu Val Asn Phe Glu Glu Ala Gly Glu Lys 
                325                 330                 335     


Gly Leu Asp Gly Ala Leu Thr Ala Lys Leu Leu Ser Lys Pro Asp His 
            340                 345                 350         


Tyr Gly Ala Ala Trp His Gly Leu Ser Phe Ala Glu Lys Asp Thr Phe 
        355                 360                 365             


Val Gly Lys Leu Leu Asp Glu Ala Asp Glu Glu Arg Leu Ile Arg Arg 
    370                 375                 380                 


Leu Val Thr Glu Asn Arg Leu Ser Glu Asp Ala Ala Arg Arg Cys Ala 
385                 390                 395                 400 


Ser Ile Pro Leu Ala Asp Gly Tyr Gly Arg Leu Gly Arg Thr Ala Asn 
                405                 410                 415     


Thr Glu Ile Leu Ala Ala Leu Val Glu Glu Thr Asp Glu Thr Gly Thr 
            420                 425                 430         


Val Val Thr Tyr Ala Glu Ala Val Arg Arg Ala Gly Glu Arg Thr Gly 
        435                 440                 445             


Arg Asn Trp His His Ser Asp Glu Arg Asp Gly Val Ile Leu Asp Arg 
    450                 455                 460                 


Leu Pro Tyr Tyr Gly Glu Ile Leu Gln Arg His Val Val Pro Gly Ser 
465                 470                 475                 480 


Gly Glu Pro Glu Glu Lys Asn Glu Ala Ala Arg Trp Gly Arg Leu Ala 
                485                 490                 495     


Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu Arg Lys Val Val Asn 
            500                 505                 510         


Arg Leu Ile Ala Ala His Gly Arg Pro Asp Gln Ile Val Val Glu Leu 
        515                 520                 525             


Ala Arg Glu Leu Lys Leu Asn Arg Glu Gln Lys Glu Arg Leu Asp Arg 
    530                 535                 540                 


Glu Asn Arg Lys Asn Arg Glu Glu Asn Glu Arg Arg Thr Ala Ile Leu 
545                 550                 555                 560 


Ala Glu His Gly Gln Arg Asp Thr Ala Glu Asn Lys Ile Arg Leu Arg 
                565                 570                 575     


Leu Phe Glu Glu Gln Ala Arg Ala Asn Ala Gly Ile Ala Leu Cys Pro 
            580                 585                 590         


Tyr Thr Gly Arg Ala Ile Gly Ile Ala Glu Leu Phe Thr Ser Glu Val 
        595                 600                 605             


Glu Ile Asp His Ile Leu Pro Val Ser Leu Thr Leu Asp Asp Ser Leu 
    610                 615                 620                 


Ala Asn Arg Val Leu Cys Arg Arg Glu Ala Asn Arg Glu Lys Arg Arg 
625                 630                 635                 640 


Gln Thr Pro Phe Gln Ala Phe Gly Ala Thr Pro Ala Trp Asn Asp Ile 
                645                 650                 655     


Val Ala Arg Ala Ala Lys Leu Pro Pro Asn Lys Arg Trp Arg Phe Asp 
            660                 665                 670         


Pro Ala Ala Leu Glu Arg Phe Glu Arg Glu Gly Gly Phe Leu Gly Arg 
        675                 680                 685             


Gln Leu Asn Glu Thr Lys Tyr Leu Ser Arg Leu Ala Lys Ile Tyr Leu 
    690                 695                 700                 


Gly Lys Ile Cys Asp Pro Asp Arg Val Tyr Val Thr Pro Gly Thr Leu 
705                 710                 715                 720 


Thr Gly Leu Leu Arg Ala Arg Trp Gly Leu Asn Ser Ile Leu Ser Asp 
                725                 730                 735     


Ser Asn Phe Lys Asn Arg Ser Asp His Arg His His Ala Val Asp Ala 
            740                 745                 750         


Val Val Ile Gly Val Leu Thr Arg Gly Met Ile Gln Arg Ile Ala His 
        755                 760                 765             


Asp Ala Ala Arg Ala Glu Asp Gln Asp Leu Asp Arg Val Phe Arg Asp 
    770                 775                 780                 


Val Pro Val Pro Phe Glu Asp Phe Arg Asp His Val Arg Glu Arg Val 
785                 790                 795                 800 


Ser Thr Ile Thr Val Ala Val Lys Pro Glu His Gly Lys Gly Gly Ala 
                805                 810                 815     


Leu His Glu Asp Thr Ser Tyr Gly Leu Val Pro Asp Thr Asp Pro Asn 
            820                 825                 830         


Ala Ala Leu Gly Asn Leu Val Val Arg Lys Pro Ile Arg Ser Leu Thr 
        835                 840                 845             


Ala Gly Glu Val Asp Arg Val Arg Asp Arg Ala Leu Arg Ala Arg Leu 
    850                 855                 860                 


Gly Ala Leu Ala Ala Pro Phe Arg Asp Glu Ser Gly Arg Val Arg Asp 
865                 870                 875                 880 


Ala Lys Gly Leu Ala Gln Ala Leu Glu Ala Phe Gly Ala Glu Asn Gly 
                885                 890                 895     


Ile Arg Arg Val Arg Ile Leu Lys Pro Asp Ala Ser Val Val Thr Ile 
            900                 905                 910         


Ala Asp Arg Arg Thr Gly Val Pro Tyr Arg Ala Val Ala Pro Gly Glu 
        915                 920                 925             


Asn His His Val Asp Ile Val Gln Met Arg Asp Gly Ser Trp Arg Gly 
    930                 935                 940                 


Phe Ala Ala Ser Val Phe Glu Val Asn Arg Pro Gly Trp Arg Pro Glu 
945                 950                 955                 960 


Trp Glu Val Lys Lys Leu Gly Gly Lys Leu Val Met Arg Leu His Lys 
                965                 970                 975     


Gly Asp Met Val Glu Leu Ser Asp Lys Asp Gly Gln Arg Arg Val Lys 
            980                 985                 990         


Val Val Gln Gln Ile Glu Ile Ser  Ala Asn Arg Val Arg  Leu Ser Pro 
        995                 1000                 1005             


His Asn  Asp Gly Gly Lys Leu  Gln Asp Arg His Ala  Asp Ala Asp 
    1010                 1015                 1020             


Asp Pro  Phe Arg Trp Asp Leu  Ala Thr Ile Pro Leu  Leu Lys Asp 
    1025                 1030                 1035             


Arg Gly  Cys Val Ala Val Arg  Val Asp Pro Ile Gly  Val Val Thr 
    1040                 1045                 1050             


Leu Arg  Arg Ser Asn Val 
    1055                 


<210>  548
<211>  1060
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  548

Met Ala Val Lys Ser Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Cys Gly Trp Ala Val Val Glu Met Asp Glu Gln Glu Asn Pro 
            20                  25                  30          


Leu Arg Leu Ile Asp Val Gly Val Arg Thr Phe Glu Glu Ala Glu Asn 
        35                  40                  45              


Pro Lys Asp Gly Ser Ser Leu Ala Glu Thr Arg Arg Leu Ala Arg Ala 
    50                  55                  60                  


Gln Arg Arg Leu Ile Ser Arg Arg Ala Asn Arg Met Asn Lys Leu Arg 
65                  70                  75                  80  


Arg Leu Leu Lys Gln Glu Asn Val Leu Gln Ala Asn Asp Tyr Asp Glu 
                85                  90                  95      


Lys Gly Leu Ile Ile Gly Leu Pro Asn Gln Ala Trp Glu Leu Arg Ala 
            100                 105                 110         


Gln Gly Leu Asp Arg Lys Leu Glu Pro Lys Glu Trp Ala Ala Val Leu 
        115                 120                 125             


Ile His Leu Val Lys His Arg Gly Tyr Leu Ser Gln Arg Lys Asn Glu 
    130                 135                 140                 


Ser Gln Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Ser Gly Met Asn 
145                 150                 155                 160 


His Asn His Gln Leu Leu Gln Ser Gln Ser Asn Gln Tyr Arg Thr Pro 
                165                 170                 175     


Ala Glu Leu Ala Ile Lys Gln Phe Ala Thr Gln Asp Gly His Ile Arg 
            180                 185                 190         


Asn Gln Arg Gly Ala Tyr Thr His Thr Phe Asn Arg Leu Asp Leu Gln 
        195                 200                 205             


Asn Glu Leu Asn Gln Leu Phe Asp Ala Gln Thr Arg Leu Gly Asn Pro 
    210                 215                 220                 


His Val Ser Gly Ser Leu Lys Thr Ala Ile Asn Asp Leu Leu Met Asn 
225                 230                 235                 240 


Gln Lys Ala Ala Leu Ser Gly Asp Ala Ile Leu Glu Met Leu Gly Arg 
                245                 250                 255     


Cys Thr Phe Glu Pro Ser Glu Tyr Lys Ala Ala Lys Asn Thr Tyr Ser 
            260                 265                 270         


Ala Glu Arg Phe Val Trp Leu Gly Lys Leu Thr Asn Leu Arg Ile Gln 
        275                 280                 285             


Asp Asn Gly Leu Glu Ser Ala Leu Ser Arg Glu Gln Arg Glu Leu Leu 
    290                 295                 300                 


Leu Asn Glu Pro Tyr Asn Lys Thr Lys Leu Thr Tyr Gln Gln Val Arg 
305                 310                 315                 320 


Lys Leu Leu Arg Leu Pro Glu Thr Ala Phe Phe Lys Gly Leu Arg Tyr 
                325                 330                 335     


Gly Lys Asp Asn Ala Glu Ser Ala Thr Leu Met Glu Met Lys Ser Tyr 
            340                 345                 350         


His Ala Ile Arg Lys Ser Leu Glu Asn Lys Ser Leu Lys Ser Glu Trp 
        355                 360                 365             


Glu Lys Leu Lys Thr Gln Pro Lys Leu Leu Asp Leu Ile Gly Thr Ala 
    370                 375                 380                 


Phe Ser Ile Tyr Lys Thr Asp Asp Glu Ile Ala Gly Cys Leu Lys Asn 
385                 390                 395                 400 


Gln Leu Pro Glu Asn Val Ile Ile Ala Leu Gln Glu Gln Leu Asn Phe 
                405                 410                 415     


Ser Asn Phe Met Glu Leu Ser Leu Lys Ala Leu Glu Lys Leu Leu Pro 
            420                 425                 430         


Leu Met Glu Gln Gly Leu Arg Tyr Asp Glu Ala Cys Thr Gln Ile Tyr 
        435                 440                 445             


Gly Asn Arg His Glu Lys Ile Ala Asp Lys Thr Glu Lys Phe Leu Pro 
    450                 455                 460                 


Asn Ile Pro Ala Asp Lys Leu Arg Asn Pro Val Val Leu Arg Thr Leu 
465                 470                 475                 480 


Thr Gln Ala Arg Lys Val Ile Asn Ala Ile Ile Arg Arg Tyr Gly Ser 
                485                 490                 495     


Pro Ala Arg Val His Ile Glu Thr Gly Arg Glu Val Gly Lys Ser Phe 
            500                 505                 510         


Ser Asp Arg Asp Lys Leu Lys Lys Gln Gln Glu Glu Asn Gln Glu Gln 
        515                 520                 525             


Arg Arg Arg Ala Ile Asp Glu Trp Lys Gln Phe Phe Pro Asn Ala Ala 
    530                 535                 540                 


His Glu Pro Lys Ala Val Asp Ile Leu Lys Leu Arg Leu Tyr Gln Leu 
545                 550                 555                 560 


Gln Gln Gly Lys Cys Leu Tyr Ser Gly Leu Pro Ile Asp Val Arg Arg 
                565                 570                 575     


Leu Pro Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe Ser 
            580                 585                 590         


Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Ile Leu Val Leu Ala Ser 
        595                 600                 605             


Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Leu Arg Gly 
    610                 615                 620                 


Ala Asp Asn Ser Glu Ser Trp Arg Leu Phe Val Glu Asn Val Gln Asn 
625                 630                 635                 640 


Cys Gln Phe Ser Pro Asn Lys Lys Gln Lys Ile Met Ala Lys Gln Leu 
                645                 650                 655     


Asp Glu Lys Gly Phe Leu Glu Arg Asn Leu Asn Asp Thr Arg Tyr Val 
            660                 665                 670         


Ser Arg Phe Leu Cys Gln Phe Ile Ser Glu Tyr Leu His Leu Thr Gly 
        675                 680                 685             


Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Ala Leu 
    690                 695                 700                 


Leu Arg Gly Arg Trp Gly Leu Ser Lys Asn Arg Glu Glu Asn Asp Arg 
705                 710                 715                 720 


His His Ala Val Asp Ala Val Val Val Ala Cys Ser Thr Val Ala Met 
                725                 730                 735     


Gln Gln Lys Ile Thr Gln Phe Val Arg Tyr Gln Lys Gly Asn Val Phe 
            740                 745                 750         


Thr Gly Glu Arg Ile Asp Lys Glu Thr Gly Glu Val Ile Ser Ile His 
        755                 760                 765             


Phe Pro Thr Pro Trp Glu His Phe Arg Glu Glu Val Met Ile Arg Val 
    770                 775                 780                 


Phe Asp Asp Asn Pro Leu Arg Thr Leu Pro Glu Lys Leu Pro Asn Arg 
785                 790                 795                 800 


Pro Glu Ala Leu His Glu Tyr Val Met Pro Leu Phe Val Ser Arg Met 
                805                 810                 815     


Pro Thr Arg Lys Met Thr Gly Gln Gly His Lys Glu Thr Ile Lys Ser 
            820                 825                 830         


Ala Lys Arg Leu Asp Glu Lys Ile Ser Val Leu Arg Val Pro Leu Thr 
        835                 840                 845             


Gln Leu Lys Leu Asn Asp Leu Glu Asn Met Val Asn Arg Glu Arg Glu 
    850                 855                 860                 


Pro Ala Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Gln Phe Lys Asn 
865                 870                 875                 880 


Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Phe Lys Gln Gly Gly Gln 
                885                 890                 895     


Gln Val Lys Ala Val Arg Val Glu Ile Val Gln Lys Ser Gly Met Leu 
            900                 905                 910         


Leu Arg Lys Ile Asn Gly Val Ala Asp Asn Gly Ser Met Val Arg Val 
        915                 920                 925             


Asp Val Phe Glu Lys Ser Gly Lys Phe Tyr Leu Val Pro Ile Tyr Ala 
    930                 935                 940                 


Trp Gln Val Ala Glu Gly Ile Leu Pro Asn His Ala Val Val His Ser 
945                 950                 955                 960 


Lys Asp Glu Ser Asp Trp Ile Leu Met Asp Glu Ser Tyr His Phe Lys 
                965                 970                 975     


Phe Ala Leu Tyr Lys Asn Asp Leu Leu Glu Val Gln Thr Lys Lys Gly 
            980                 985                 990         


Cys Ile Met Gly Tyr Phe Ala Ser  Leu Asp Arg Ala Thr  Gly Ala Ile 
        995                 1000                 1005             


Thr Ile  Arg Glu His Asp Arg  Asp Lys Lys Lys Cys  Lys Asp Gly 
    1010                 1015                 1020             


Glu His  Arg Ser Ile Gly Val  Lys Met Ala Ser Ser  Phe Lys Lys 
    1025                 1030                 1035             


Leu Gln  Val Asp Glu Leu Gly  Lys Asn Ile His Glu  Thr Arg Ser 
    1040                 1045                 1050             


Arg Lys  Arg Pro Asn Ile Gly  
    1055                 1060 


<210>  549
<211>  1061
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  549

Met His Thr Leu Pro Tyr Thr Leu Gly Leu Asp Ile Gly Ile Ala Ser 
1               5                   10                  15      


Val Gly Ala Ala Leu Leu Ser Asp Glu Ser Ile Leu Gly Met His Val 
            20                  25                  30          


Arg Thr Phe Ser Ala Ala Glu Thr Asp Lys Glu Gly Asp Pro Leu Asn 
        35                  40                  45              


Lys Ile Arg Arg Glu Ala Arg Ser Ala Arg Arg Arg Ile Arg Arg Arg 
    50                  55                  60                  


Ala His Arg Leu Leu Arg Leu Arg Arg Leu Leu Lys Arg Thr Gly Leu 
65                  70                  75                  80  


Ile Ser Asp Thr Ser Leu Glu Val Leu Lys Thr Ser Lys Thr Pro Trp 
                85                  90                  95      


Gln Leu Arg Ser Glu Gly Leu Asp Arg Leu Leu Ser Pro Ser Glu Leu 
            100                 105                 110         


Ala Ala Ala Leu Tyr His Ile Val Lys His Arg Gly Phe Gln Ser Asn 
        115                 120                 125             


Arg Lys Ala Glu Leu Lys Thr Asp Ala Lys Ala Gly Gln Met Leu Ser 
    130                 135                 140                 


Gly Val Ser Ala Asn Gln Gln Leu Met Arg Asp Lys Ser Tyr Arg Thr 
145                 150                 155                 160 


Ile Ala Glu Met Val Cys Lys Asp Asp Ala Phe Lys Asp His Lys Arg 
                165                 170                 175     


Asn Lys Gly Gly Ser Tyr Arg Asn Thr Phe Ala Arg Ser Asp Leu Ala 
            180                 185                 190         


Asp Glu Leu Lys Thr Leu Leu Lys Lys Gln Arg Glu Tyr Asp Asn Pro 
        195                 200                 205             


Leu Ala Thr Pro Glu Leu Glu Glu Ala Val His Thr Leu Leu Met Ala 
    210                 215                 220                 


Arg Arg Pro Val Phe Lys Asp Asp Asp Ile Glu Lys Leu Val Gly Phe 
225                 230                 235                 240 


Cys Thr Phe Glu His Gly Glu Lys Arg Ala Pro Lys Ala Ala Phe Ser 
                245                 250                 255     


Ser Glu Arg Phe Ile Trp Leu Gly Lys Leu Asn Asn Leu Arg Leu Ile 
            260                 265                 270         


Tyr Pro Gly Glu Gln Arg Ser Leu Thr Pro Glu Glu Arg Ser Gln Ile 
        275                 280                 285             


Ile Asn Leu Pro Phe Thr Gln Ala Ser Val Lys Phe Ser Gln Ile Arg 
    290                 295                 300                 


Lys Lys Leu Asp Leu Pro Glu Tyr Val Arg Phe Asn Leu Val Arg Tyr 
305                 310                 315                 320 


Lys Pro Gly Glu Glu Thr Ser Lys Ser Glu Thr Ala Thr Phe Phe Glu 
                325                 330                 335     


Ala Lys Ala Phe His Glu Leu Arg Lys Thr Tyr Arg Glu Ser Gly Ile 
            340                 345                 350         


Ser Glu Leu Trp Glu Arg Asp Gln His Asp Pro Gln Arg Leu Asp Gln 
        355                 360                 365             


Ile Ala Thr Ala Ile Ala Val Tyr Arg Asp Asp Glu Arg Ile Arg Asn 
    370                 375                 380                 


Glu Leu Ser Pro His Thr Ile Glu Asn Glu Val Val Glu Ala Val Leu 
385                 390                 395                 400 


Asn Cys Asp Phe Thr Gln Phe Ile Asn Leu Ser Leu Lys Ala Leu Asn 
                405                 410                 415     


Lys Ile Leu Pro Tyr Met Glu Gln Gly Gln Arg Tyr Asp Glu Ala Cys 
            420                 425                 430         


Thr Ser Ala Gly Tyr Gln His His Ser Pro Gln Gln Asn Glu Glu Lys 
        435                 440                 445             


Ser Gly Arg Ile Pro Pro Pro Asp Lys Asn Val Ile Arg Asn Pro Val 
    450                 455                 460                 


Val Tyr Arg Ser Leu Asn Gln Ala Arg Lys Leu Ile Asn Ala Ile Thr 
465                 470                 475                 480 


Lys Arg Tyr Gly Ala Pro Ala Ala Ile His Leu Glu Met Ala Arg Asp 
                485                 490                 495     


Leu Ser Lys Pro Phe Ser Glu Arg Lys Ser Ile Glu Arg Glu Gln Glu 
            500                 505                 510         


Lys Tyr Arg Lys Gln Lys Val Gln Leu Arg Asn Glu Tyr Ile Glu His 
        515                 520                 525             


Phe Gly Thr Glu Pro Asn Gly Leu Asn Leu Leu Lys Phe Arg Leu Tyr 
    530                 535                 540                 


Lys Glu Gln Asp Ala Gln Cys Ala Tyr Thr Gln Lys Ala Leu Asp Leu 
545                 550                 555                 560 


Ser Arg Leu Asn Glu Ala Gly Tyr Val Glu Val Asp His Ile Leu Pro 
                565                 570                 575     


Tyr Ser Arg Ser Tyr Asp Asp Ser Leu Leu Asn Lys Thr Leu Val Leu 
            580                 585                 590         


Thr Glu Ala Asn Arg Asp Lys Gly Asn Arg Thr Pro Phe Glu Phe Met 
        595                 600                 605             


Gly Gly Asp Asp Gln Ser Gln His Trp Gln Lys Phe Val Arg Trp Val 
    610                 615                 620                 


Gln Met Asn Pro Lys Ile Arg Gln Ala Lys Arg Asn Arg Tyr Leu Arg 
625                 630                 635                 640 


Lys Asp Phe Ser Glu Lys Ala Ala Arg Glu Phe Arg Asp Arg His Leu 
                645                 650                 655     


Asn Asp Thr Arg Tyr Ile Gly Arg Glu Leu Lys Ala Met Ile Glu Ser 
            660                 665                 670         


His Val Gln Trp His Pro Asp Ser Lys Ser Lys Asp Cys Cys Val Val 
        675                 680                 685             


Leu Ser Gly Gln Leu Thr Ala Leu Leu Arg Gly Arg Trp Gly Phe Asn 
    690                 695                 700                 


Lys Asp Arg Ala Ala Gly Asp Leu His His Ala Val Asp Ala Ala Val 
705                 710                 715                 720 


Val Ala Ala Ala Thr Arg Gly Met Ile Gln Arg Met Ala Arg His Ser 
                725                 730                 735     


Lys Phe His Glu Leu Lys Asn Val Arg Thr Ser Tyr Ala Asp Pro Asp 
            740                 745                 750         


Thr Gly Glu Val Val Asn Leu Ala Ala Ala Arg Asp Leu Glu Lys His 
        755                 760                 765             


Phe Pro Leu Pro Trp Asp Asn Phe Arg Asn Asp Thr Leu Ala Tyr Leu 
    770                 775                 780                 


Asn Pro Ser Ala Ile Ser Ala Asp Glu Ser Ala Gln Glu Gly His Arg 
785                 790                 795                 800 


Gly Val Arg Val Ser Arg Ala Pro Thr Arg Arg Lys Leu Gly Ala Ala 
                805                 810                 815     


His Gln Glu Thr Ile Arg Ser Lys Gly Lys Asp Asn Ile Leu Leu Glu 
            820                 825                 830         


Gln Gly Leu Ser Ala Val Lys Thr Pro Leu Ser Lys Leu Thr Leu Lys 
        835                 840                 845             


Asn Ile Glu Asn Met Val Gly Tyr Glu Asp Pro Arg Asn Ala Ala Leu 
    850                 855                 860                 


Val Lys Val Leu Arg Glu Arg Leu Glu Ala Phe Gly Gly Asp Gly Ala 
865                 870                 875                 880 


Lys Ala Phe Ala Pro Asn Gln Pro Pro Val Tyr Lys Pro Ser Lys Pro 
                885                 890                 895     


Gly Lys Gln Ala Pro Glu Ile Arg Ser Val Lys Met Ala Ser Lys Ser 
            900                 905                 910         


Lys Gly Ala Leu His Val Arg Glu Gly Leu Ala Asp Ser Gly Arg Met 
        915                 920                 925             


Ile Arg Ile Asp Ile Tyr Glu Thr Lys Asn Gly Tyr Val Gly Val Pro 
    930                 935                 940                 


Ile Tyr Val Ser Asp Lys Ala Arg Ser Lys Leu Pro Lys Ala Tyr Pro 
945                 950                 955                 960 


Lys Lys Gly Glu Asp Arg Ala Asn Trp Pro Glu Val Asp Thr Asn Asp 
                965                 970                 975     


Asn Phe Leu Phe Ser Leu Tyr Ser Thr Asp Trp Ile Glu Ala Lys Thr 
            980                 985                 990         


Lys Lys Glu Thr Ile Ala Gly Tyr  Val Lys Ser Tyr Asp  Ile Phe Asp 
        995                 1000                 1005             


Gly Arg  Leu Leu Val Tyr Ala  His Asp Asn Lys Lys  Val Val Arg 
    1010                 1015                 1020             


Lys Leu  Thr Leu Ser Ala Leu  Ala Asp Leu Gln Lys  Leu His Val 
    1025                 1030                 1035             


Ser Ile  Leu Gly Asp Leu Tyr  Lys Gly Gly Thr Pro  Lys Cys Ser 
    1040                 1045                 1050             


Pro Leu  Arg Lys Gly Gln Glu  Leu 
    1055                 1060     


<210>  550
<211>  1061
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  550

Met Leu Arg Leu Gly Leu Asp Ile Gly Ser Thr Ser Ile Gly Trp Phe 
1               5                   10                  15      


Leu Tyr Arg Ile Thr Asn Gly Met Ile Ala Glu Ile Ile Asp Cys Gly 
            20                  25                  30          


Val Arg Leu Phe Ser Asp Gly Arg Asp Ala Lys Thr Gly Val Ser Ser 
        35                  40                  45              


Ala Val Glu Arg Arg Asn Ala Arg Ala Leu Arg Arg Arg Arg Asp Arg 
    50                  55                  60                  


Tyr Leu Arg Arg Arg Ala Ala Leu Met Arg Arg Leu Ala Ala Ala Gly 
65                  70                  75                  80  


Leu Met Pro Glu Asp Pro Ser Gln Ala Lys Pro Leu Glu Leu Leu Asp 
                85                  90                  95      


Pro Tyr Arg Leu Arg Ala Asp Ala Leu Asp Arg Arg Leu Glu Leu Thr 
            100                 105                 110         


Glu Leu Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Gln Thr Asp Arg Gly Asn Asn Glu Gly Gly Lys Ile Ile 
    130                 135                 140                 


Asp Gly Ser Ala Arg Leu Asp Gln Ala Met Met Ala Val Gly Ala Arg 
145                 150                 155                 160 


Thr Tyr Gly Glu Phe Leu His Leu Arg Arg Ser Gln Ala Glu Asp Leu 
                165                 170                 175     


Arg Arg Val Pro Ser Val Arg Thr Arg Leu Thr Leu Arg Ser Val Asp 
            180                 185                 190         


Gly Gly Lys Ala Glu Thr Gly Tyr Asp Phe Tyr Pro Asp Arg Arg His 
        195                 200                 205             


Leu Glu Glu Glu Phe Asn Lys Val Trp Ala Ala Gln Ala Ala His His 
    210                 215                 220                 


Pro Val Leu Thr Asp Ser Leu Arg His Glu Val Phe Glu Thr Ile Phe 
225                 230                 235                 240 


Phe Gln Arg Pro Leu Lys Ala Pro Ala Val Gly Arg Cys Leu Phe Phe 
                245                 250                 255     


Pro Glu Pro Arg Leu Pro Arg Ala His Pro Leu Thr Gln Arg Arg Val 
            260                 265                 270         


Leu Tyr Glu Thr Val Asn Ala Leu Arg Ile Arg Thr Glu Ser Gly Ala 
        275                 280                 285             


Ala Val Pro Leu Gly Lys Glu Gln Arg Asp Lys Val Ile Met Leu Leu 
    290                 295                 300                 


Asp Gly Lys Ala Pro Val Lys Ala Ile Ala Ser Ala Lys Leu Ser Leu 
305                 310                 315                 320 


Lys Ala Ile Ala Lys His Leu Lys Leu Arg Asp Gly Gln Thr Phe Thr 
                325                 330                 335     


Leu Glu Lys Gly Arg Asp Tyr Ile Ala Cys Asp Pro Val Arg Ala Ser 
            340                 345                 350         


Leu Ser His Pro Asp Arg Phe Gly Pro Arg Trp Ser Val Leu Glu Ala 
        355                 360                 365             


Glu Val Gln Ala Glu Ile Val Gly Arg Ile Arg Asp Asp Asp Asp Thr 
    370                 375                 380                 


Met Ala Leu Val Glu Trp Leu Lys Gln Val His Gly Leu Asp Asp Ala 
385                 390                 395                 400 


His Ala Arg Ala Thr Ala Asp Ala Pro Leu Pro Glu Gly Tyr Gly Arg 
                405                 410                 415     


Leu Gly Glu Thr Ala Thr Arg Lys Leu Leu Asp Cys Leu Lys Ala Glu 
            420                 425                 430         


Val Leu Thr Tyr Ser Glu Ala Val Gly Lys Ala Leu Asp Gln His His 
        435                 440                 445             


Ser Asp Arg Arg Thr Gly Glu Ile Leu Glu Ser Leu Pro Tyr Tyr Gly 
    450                 455                 460                 


Glu Val Leu Gln Ala His Val Ile Pro Gly Thr Gly Glu Pro His His 
465                 470                 475                 480 


Asp Asp Ile Thr Arg Phe Gly Arg Ile Thr Asn Pro Thr Val His Ile 
                485                 490                 495     


Gly Leu Asn Gln Leu Arg Arg Leu Val Asn Arg Ile Ile Ala Arg Tyr 
            500                 505                 510         


Gly Lys Pro Ala Glu Ile Val Val Glu Leu Ala Arg Asp Leu Lys Gln 
        515                 520                 525             


Ser Thr Gly Gln Lys Lys Ala Ala Ile Ala Leu Asn Leu Lys Asn Arg 
    530                 535                 540                 


Lys Ala Ala Glu Ala Arg Ser Ala Leu Leu Thr Glu Trp Gly Val Pro 
545                 550                 555                 560 


Asp Thr Gly Ala Asn Arg Met Tyr Val Arg Leu Trp Gln Glu Leu His 
                565                 570                 575     


Glu Asp Pro Met Met Arg Arg Cys Pro Tyr Thr Gly Thr Ile Ile Ser 
            580                 585                 590         


Pro Ser Leu Leu Phe Ser Gly Ala Ile Asp Ile Asp His Ile Leu Pro 
        595                 600                 605             


Tyr Ser Arg Thr Leu Asp Asp Ser Thr Ala Asn Arg Thr Leu Cys Met 
    610                 615                 620                 


Lys Glu Ala Asn Arg Gln Lys Arg Asn Lys Ser Pro Ser Glu Ala Arg 
625                 630                 635                 640 


Arg Asp Gly Met Arg Ile Trp Ala Leu Glu Asp Asp Asn Leu Arg His 
                645                 650                 655     


Ile Pro Glu Phe Lys Arg Trp Arg Phe Ala Glu Asp Ala Met Ala Arg 
            660                 665                 670         


Phe Glu Gly Glu Arg Asp Phe Glu Ala Arg Ala Leu Val Asp Thr Gln 
        675                 680                 685             


Tyr Leu Ser Arg Ile Ser Lys Ala Tyr Leu Glu Thr Leu Tyr Asp Gly 
    690                 695                 700                 


Ala Asp Gly Lys Ser His Val Trp Val Val Pro Gly Arg Leu Thr Glu 
705                 710                 715                 720 


Met Leu Arg Arg His Trp Gly Leu Asn Gly Ile Leu Thr Asp Pro Leu 
                725                 730                 735     


Arg Asp Val Thr Lys Glu Lys Asn Arg Met Asp His Arg His His Ala 
            740                 745                 750         


Ile Asp Ala Ala Val Val Ala Ala Thr Asp Arg Gly Leu Ile Lys Ala 
        755                 760                 765             


Met Ala Glu Gln Ala Arg Thr Asp Val Asp Gln Gly Arg Glu Ala Leu 
    770                 775                 780                 


Ala Ser Ser Ile Met Pro Pro Trp Pro Gly Phe Arg Glu Ala Val Ala 
785                 790                 795                 800 


Ala Gln Val Asp Arg Ile Ile Val Ser His Arg Pro Asp His Gly Arg 
                805                 810                 815     


Ile Asp Leu Thr Thr Arg Met Lys Gly Arg Asp Gln Thr Thr Gly Pro 
            820                 825                 830         


Leu His Arg Asp Thr Ala Tyr Gly Leu Thr Gly Gln Ser Glu Lys Gly 
        835                 840                 845             


Val Pro Leu Val Val Thr Arg Glu Pro Phe Asp Asn Leu Thr Pro Thr 
    850                 855                 860                 


Met Ile Gly Lys Ile Arg Asp Glu Pro Leu Lys Ala Ala Leu Ala Ala 
865                 870                 875                 880 


Val Thr Ala Gly Lys Lys Asp Asp Ala Phe Ala Glu Ala Leu Ala His 
                885                 890                 895     


Phe Arg Asp Arg Pro Gly Pro Tyr His Gly Ile Arg Arg Val Arg Leu 
            900                 905                 910         


Ile Glu Pro Ile Ser Val Val Glu Ile Arg Asp Ala Ser Gly Arg Ala 
        915                 920                 925             


Tyr Lys Gly Tyr Lys Gly Asn Ser Asn His Cys Phe Glu Val Trp Arg 
    930                 935                 940                 


Leu Pro Gly Gly Ala Ile Thr Glu His Ile Val Ser Thr Phe Asp Ala 
945                 950                 955                 960 


His Gln Pro Gly Pro Glu Pro Arg Pro His Pro Ala Ala Lys Arg Leu 
                965                 970                 975     


Leu Arg Leu Phe Lys Ser Asp Met Val Lys Leu Asp Asp Ser Lys Phe 
            980                 985                 990         


Gly Pro Val Ile Ala Thr Val Val  Met Phe Asp Arg Asn  Gly Lys Ile 
        995                 1000                 1005             


Ser Leu  Val Pro His Thr Ala  Ser Asn Ala Arg Lys  Arg Cys Glu 
    1010                 1015                 1020             


Lys Tyr  Lys Glu Asp Leu Tyr  Leu Arg Phe Thr Ala  Arg Thr Leu 
    1025                 1030                 1035             


Ile Glu  Ala Gly Ala Arg Arg  Ile Ile Val Asp Glu  Thr Gly His 
    1040                 1045                 1050             


Phe Arg  Asp Pro Gly Arg Lys  Gly 
    1055                 1060     


<210>  551
<211>  1062
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  551

Met Ala Glu Lys Gln His Arg Trp Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Ile Gly Trp Ala Val Ile Ala Leu Ile Glu Gly Arg Pro Ala Gly Leu 
            20                  25                  30          


Val Ala Thr Gly Ser Arg Ile Phe Ser Asp Gly Arg Asn Pro Lys Asp 
        35                  40                  45              


Gly Ser Ser Leu Ala Val Glu Arg Arg Gly Pro Arg Gln Met Arg Arg 
    50                  55                  60                  


Arg Arg Asp Arg Tyr Leu Arg Arg Arg Asp Arg Phe Met Gln Ala Leu 
65                  70                  75                  80  


Ile Asn Val Gly Leu Met Pro Gly Asp Ala Ala Ala Arg Lys Ala Leu 
                85                  90                  95      


Val Thr Glu Asn Pro Tyr Val Leu Arg Gln Arg Gly Leu Asp Gln Ala 
            100                 105                 110         


Leu Thr Leu Pro Glu Phe Gly Arg Ala Leu Phe His Leu Asn Gln Arg 
        115                 120                 125             


Arg Gly Phe Gln Ser Asn Arg Lys Thr Asp Arg Ala Thr Ala Lys Glu 
    130                 135                 140                 


Ser Gly Lys Val Lys Asn Ala Ile Ala Ala Phe Arg Ala Gly Met Gly 
145                 150                 155                 160 


Asn Ala Arg Thr Val Gly Glu Ala Leu Ala Arg Arg Leu Glu Asp Gly 
                165                 170                 175     


Arg Pro Val Arg Ala Arg Met Val Gly Gln Gly Lys Asp Glu His Tyr 
            180                 185                 190         


Glu Leu Tyr Ile Ala Arg Glu Trp Ile Ala Gln Glu Phe Asp Ala Leu 
        195                 200                 205             


Trp Ala Ser Gln Gln Arg Phe His Ala Glu Val Leu Ala Asp Ala Ala 
    210                 215                 220                 


Arg Asp Arg Leu Arg Ala Ile Leu Leu Phe Gln Arg Lys Leu Leu Pro 
225                 230                 235                 240 


Val Pro Val Gly Lys Cys Phe Leu Glu Pro Asn Gln Pro Arg Val Ala 
                245                 250                 255     


Ala Ala Leu Pro Ser Ala Gln Arg Phe Arg Leu Met Gln Glu Leu Asn 
            260                 265                 270         


His Leu Arg Val Met Thr Leu Ala Asp Lys Arg Glu Arg Pro Leu Ser 
        275                 280                 285             


Phe Gln Glu Arg Asn Asp Leu Leu Ala Gln Leu Val Ala Arg Pro Lys 
    290                 295                 300                 


Cys Gly Phe Asp Met Leu Arg Lys Ile Val Phe Gly Ala Asn Lys Glu 
305                 310                 315                 320 


Ala Tyr Arg Phe Thr Ile Glu Ser Glu Arg Arg Lys Glu Leu Lys Gly 
                325                 330                 335     


Cys Asp Thr Ala Ala Lys Leu Ala Lys Val Asn Ala Leu Gly Thr Arg 
            340                 345                 350         


Trp Gln Ala Leu Ser Leu Asp Glu Gln Asp Arg Leu Val Cys Leu Leu 
        355                 360                 365             


Leu Asp Gly Glu Asn Asp Ala Val Leu Ala Asp Ala Leu Arg Glu His 
    370                 375                 380                 


Tyr Gly Leu Thr Asp Ala Gln Ile Asp Thr Leu Leu Gly Leu Ser Phe 
385                 390                 395                 400 


Glu Asp Gly His Met Arg Leu Gly Arg Ser Ala Leu Leu Arg Val Leu 
                405                 410                 415     


Asp Ala Leu Glu Ser Gly Arg Asp Glu Gln Gly Leu Pro Leu Ser Tyr 
            420                 425                 430         


Asp Lys Ala Val Val Ala Ala Gly Tyr Pro Ala His Thr Ala Asp Leu 
        435                 440                 445             


Glu Asn Gly Glu Arg Asp Ala Leu Pro Tyr Tyr Gly Glu Leu Leu Trp 
    450                 455                 460                 


Arg Tyr Thr Gln Asp Ala Pro Thr Ala Lys Asn Asp Ala Glu Arg Lys 
465                 470                 475                 480 


Phe Gly Lys Ile Ala Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu 
                485                 490                 495     


Arg Lys Leu Val Asn Ala Leu Ile Gln Arg Tyr Gly Lys Pro Ala Gln 
            500                 505                 510         


Ile Val Val Glu Leu Ala Arg Asn Leu Lys Ala Gly Leu Glu Glu Lys 
        515                 520                 525             


Glu Arg Ile Lys Lys Gln Gln Thr Ala Asn Leu Glu Arg Asn Glu Arg 
    530                 535                 540                 


Ile Arg Gln Lys Leu Gln Asp Ala Gly Val Pro Asp Asn Arg Glu Asn 
545                 550                 555                 560 


Arg Leu Arg Met Arg Leu Phe Glu Glu Leu Gly Gln Gly Asn Gly Leu 
                565                 570                 575     


Gly Thr Pro Cys Ile Tyr Ser Gly Arg Gln Ile Ser Leu Gln Arg Leu 
            580                 585                 590         


Phe Ser Asn Asp Val Gln Val Asp His Ile Leu Pro Phe Ser Lys Thr 
        595                 600                 605             


Leu Asp Asp Ser Phe Ala Asn Lys Val Leu Ala Gln His Asp Ala Asn 
    610                 615                 620                 


Arg Tyr Lys Gly Asn Arg Gly Pro Phe Glu Ala Phe Gly Ala Asn Arg 
625                 630                 635                 640 


Asp Gly Tyr Ala Trp Asp Asp Ile Arg Ala Arg Ala Ala Val Leu Pro 
                645                 650                 655     


Arg Asn Lys Arg Asn Arg Phe Ala Glu Thr Ala Met Gln Asp Trp Leu 
            660                 665                 670         


His Asn Glu Thr Asp Phe Leu Ala Arg Gln Leu Thr Asp Thr Ala Tyr 
        675                 680                 685             


Leu Ser Arg Val Ala Arg Gln Tyr Leu Thr Ala Ile Cys Ser Lys Asp 
    690                 695                 700                 


Asp Val Tyr Val Ser Pro Gly Arg Leu Thr Ala Met Leu Arg Ala Lys 
705                 710                 715                 720 


Trp Gly Leu Asn Arg Val Leu Asp Gly Val Met Glu Glu Gln Gly Arg 
                725                 730                 735     


Pro Ala Val Lys Asn Arg Asp Asp His Arg His His Ala Ile Asp Ala 
            740                 745                 750         


Val Val Ile Gly Ala Thr Asp Arg Ala Met Leu Gln Gln Val Ala Thr 
        755                 760                 765             


Leu Ala Ala Arg Ala Arg Glu Gln Asp Ala Glu Arg Leu Ile Gly Asp 
    770                 775                 780                 


Met Pro Thr Pro Trp Pro Asn Phe Leu Glu Asp Val Arg Ala Ala Val 
785                 790                 795                 800 


Ala Arg Cys Val Val Ser His Lys Pro Asp His Gly Pro Glu Gly Gly 
                805                 810                 815     


Leu His Asn Asp Thr Ala Tyr Gly Ile Val Ala Gly Pro Phe Glu Asp 
            820                 825                 830         


Gly Arg Tyr Arg Val Arg His Arg Val Ser Leu Phe Asp Leu Lys Pro 
        835                 840                 845             


Gly Asp Leu Ser Asn Val Arg Cys Asp Ala Pro Leu Gln Ala Glu Leu 
    850                 855                 860                 


Glu Pro Ile Phe Glu Gln Asp Asp Ala Arg Ala Arg Glu Val Ala Leu 
865                 870                 875                 880 


Thr Ala Leu Ala Glu Arg Tyr Arg Gln Arg Lys Val Trp Leu Glu Glu 
                885                 890                 895     


Leu Met Ser Val Leu Pro Ile Arg Pro Arg Gly Glu Asp Gly Lys Thr 
            900                 905                 910         


Leu Pro Asp Ser Ala Pro Tyr Lys Ala Tyr Lys Gly Asp Ser Asn Tyr 
        915                 920                 925             


Cys Tyr Glu Leu Phe Ile Asn Glu Arg Gly Arg Trp Asp Gly Glu Leu 
    930                 935                 940                 


Ile Ser Thr Phe Arg Ala Asn Gln Ala Ala Tyr Arg Arg Phe Arg Asn 
945                 950                 955                 960 


Asp Pro Ala Arg Phe Arg Arg Tyr Thr Ala Gly Gly Arg Pro Leu Leu 
                965                 970                 975     


Met Arg Leu Cys Ile Asn Asp Tyr Ile Ala Val Gly Thr Ala Ala Glu 
            980                 985                 990         


Arg Thr Ile Phe Arg Val Val Lys  Met Ser Glu Asn Lys  Ile Thr Leu 
        995                 1000                 1005             


Ala Glu  His Phe Glu Gly Gly  Thr Leu Lys Gln Arg  Asp Ala Asp 
    1010                 1015                 1020             


Lys Asp  Asp Pro Phe Lys Tyr  Leu Thr Lys Ser Pro  Gly Ala Leu 
    1025                 1030                 1035             


Arg Asp  Leu Gly Ala Arg Arg  Ile Phe Val Asp Leu  Ile Gly Arg 
    1040                 1045                 1050             


Val Leu  Asp Pro Gly Ile Lys  Gly Asp 
    1055                 1060         


<210>  552
<211>  1062
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  552

Met Asn Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Glu Ile Asp Glu Gln Glu Asn Pro Gln Arg Leu 
            20                  25                  30          


Ile Asp Val Gly Val Arg Thr Phe Asp Arg Ala Glu Val Leu Lys Thr 
        35                  40                  45              


Gly Glu Ser Leu Ala Leu Ala Arg Arg Leu Ala Arg Ser Ser Arg Arg 
    50                  55                  60                  


Leu Val Lys Arg Arg Ala Glu Arg Ile Lys Lys Ala Lys Arg Leu Leu 
65                  70                  75                  80  


Lys Ala Glu Asn Leu Leu Leu Ser Ala Asp Glu Ile Leu Pro Asn Glu 
                85                  90                  95      


Ala Trp Gln Leu Arg Val Lys Gly Leu Asp Gln Lys Leu Glu Arg Gln 
            100                 105                 110         


Glu Trp Ala Ala Val Leu Leu His Leu Leu Lys His Arg Gly Tyr Leu 
        115                 120                 125             


Ser Gln Arg Lys Asn Glu Ser Lys Ser Glu Asn Lys Glu Leu Gly Ala 
    130                 135                 140                 


Leu Leu Ser Gly Val Ala Ile Asn His Gln Ile Leu Gln Ser Ala Glu 
145                 150                 155                 160 


Tyr Arg Thr Pro Ala Glu Ile Ala Val Asn Lys Phe Gln Ala Glu Glu 
                165                 170                 175     


Gly His Ile Arg Asn Gln Arg Gly Ala Tyr Thr His Thr Phe Ser Arg 
            180                 185                 190         


Leu Asp Leu Leu Ala Glu Met Glu Leu Leu Phe Gln Arg Gln Ala Thr 
        195                 200                 205             


Leu Gly Asn Pro His Thr Ser Thr Lys Leu Leu Glu Asn Leu Thr Ala 
    210                 215                 220                 


Leu Leu Met Trp Gln Lys Pro Ala Leu Ala Gly Glu Ala Ile Leu Lys 
225                 230                 235                 240 


Met Leu Gly Lys Cys Thr Phe Glu Pro Thr Glu Tyr Lys Ala Ala Lys 
                245                 250                 255     


Asn Ser Tyr Ser Ala Glu Arg Phe Val Trp Leu Thr Lys Leu Asn Asn 
            260                 265                 270         


Leu Arg Ile Leu Glu Gln Gly Ala Glu Arg Ala Leu Thr Asp Asn Glu 
        275                 280                 285             


Arg Phe Ala Leu Leu Asp Gln Pro Tyr Glu Lys Ala Lys Leu Thr Tyr 
    290                 295                 300                 


Ala Gln Val Arg Thr Leu Leu Gly Leu Ser Asp Glu Ala Ile Phe Lys 
305                 310                 315                 320 


Gly Leu Arg Tyr Gln Gly Glu Asp Lys Lys Ala Val Glu Thr Lys Thr 
                325                 330                 335     


Thr Leu Met Glu Val Lys Ala Tyr His Gln Ile Arg Lys Ala Leu Glu 
            340                 345                 350         


Gly Leu Asp Leu Lys Ala Glu Trp Asn Glu Leu Lys Asn Asn Pro Ala 
        355                 360                 365             


Leu Leu Asp Glu Ile Gly Thr Ala Phe Ser Leu Tyr Lys Thr Asp Asp 
    370                 375                 380                 


Asp Ile His Arg Tyr Leu Asp Gly Lys Leu Ser Glu Ser Val Leu Asn 
385                 390                 395                 400 


Ala Leu Leu Glu Asn Leu Asn Phe Asp Lys Phe Ile Gln Leu Ser Leu 
                405                 410                 415     


Lys Ala Leu Gln Gln Ile Leu Pro Leu Met Leu Gln Gly Gln Arg Tyr 
            420                 425                 430         


Asp Glu Ala Val Ser Ala Ile Tyr Gly Asp His Tyr Gly Lys Lys Ser 
        435                 440                 445             


Asp Glu Ile Asp Arg Leu Leu Pro Asn Ile Pro Thr Asp Glu Ile Arg 
    450                 455                 460                 


Asn Pro Val Val Leu Arg Thr Leu Thr Gln Ala Arg Lys Val Ile Asn 
465                 470                 475                 480 


Ala Val Val Arg Leu Tyr Gly Ser Pro Ala Arg Ile His Ile Glu Thr 
                485                 490                 495     


Gly Arg Glu Val Gly Lys Ser Tyr Gln Asp Arg Lys Lys Leu Glu Lys 
            500                 505                 510         


Gln Gln Glu Glu Asn Arg Lys Gln Arg Glu Asn Ala Val Lys Lys Phe 
        515                 520                 525             


Lys Glu Tyr Phe Pro Asn Phe Val Gly Glu Pro Lys Gly Lys Asp Ile 
    530                 535                 540                 


Leu Lys Met Arg Leu Tyr Glu Leu Gln Gln Ala Lys Cys Leu Tyr Ser 
545                 550                 555                 560 


Gly Lys Ser Leu Glu Leu Tyr Arg Leu Leu Glu Lys Gly Tyr Val Glu 
                565                 570                 575     


Val Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe Asn 
            580                 585                 590         


Asn Lys Val Leu Val Leu Ala Asn Glu Asn Gln Asn Lys Gly Asn Ala 
        595                 600                 605             


Thr Pro Tyr Glu Trp Leu Asp Gly Lys Asn Asn Ser Glu Gly Trp Gln 
    610                 615                 620                 


His Phe Val Ala Arg Val Gln Thr Ser Gly Phe Ser Tyr Ala Lys Lys 
625                 630                 635                 640 


Gln Arg Val Leu Ile Gln Glu Leu Asp Glu Lys Ser Phe Ile Glu Arg 
                645                 650                 655     


Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe Leu Cys Asn His Ile 
            660                 665                 670         


Ala Asp Asn Met Leu Leu Thr Gly Lys Gly Lys Arg Lys Val Phe Ala 
        675                 680                 685             


Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg Gly Cys Trp Gly Leu Gln 
    690                 695                 700                 


Lys Val Arg Glu Asn Asn Asp Arg His His Ala Leu Asp Ala Val Val 
705                 710                 715                 720 


Val Ala Cys Ser Thr Lys Ser Met Gln Asp Lys Ile Thr Lys Phe Val 
                725                 730                 735     


Lys Tyr Lys Glu Gly Arg Leu Phe Lys Asp Lys Lys Asp Gly Val Tyr 
            740                 745                 750         


Lys Gly Glu Arg Ile Asn Arg Glu Thr Gly Glu Ile Ile Ser Leu His 
        755                 760                 765             


Phe Pro Thr Pro Trp Lys Phe Phe Lys Glu Asn Val Glu Ile Arg Ile 
    770                 775                 780                 


Phe Ser Glu Asn Pro Lys Leu Glu Leu Glu Asn Arg Leu Pro Asp Tyr 
785                 790                 795                 800 


Pro Gln Tyr Asn His Glu Phe Val Gln Pro Leu Phe Val Ser Arg Met 
                805                 810                 815     


Pro Thr Arg Lys Met Thr Gly Gln Gly His Met Glu Thr Val Lys Ser 
            820                 825                 830         


Ala Lys Arg Leu Asp Lys Gly Leu Ser Val Leu Lys Val Pro Leu Thr 
        835                 840                 845             


Gln Leu Lys Leu Ser Asp Leu Glu Arg Met Val Asn Arg Glu Arg Glu 
    850                 855                 860                 


Met Ala Leu Tyr Glu Ala Leu Lys Glu Cys Leu Glu Gln Phe Gly Asn 
865                 870                 875                 880 


Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Lys Gly Gly Ala 
                885                 890                 895     


Leu Val Lys Ala Val Arg Val Glu Gln Thr Gln Lys Ser Gly Val Leu 
            900                 905                 910         


Val Arg Asn Gly Asn Gly Val Ala Asp Asn Ala Thr Gly Ser Ile Val 
        915                 920                 925             


Arg Val Asp Val Phe Thr Lys Gly Gly Lys Tyr Phe Leu Val Pro Ile 
    930                 935                 940                 


Tyr Thr Trp Gln Val Ala Lys Gly Ile Leu Pro Asn Lys Ala Val Ile 
945                 950                 955                 960 


Gln Gly Lys Asp Glu Ser Glu Trp Glu Glu Met Asp Glu Asn Ala Val 
                965                 970                 975     


Phe Gln Phe Ser Met His Lys Asn Asp Leu Ile Lys Val Lys Thr Lys 
            980                 985                 990         


Lys Gly Glu Ile Phe Gly Tyr Phe  Asn Gly Leu Asp Arg  Ala Thr Gly 
        995                 1000                 1005             


Ser Ile  Glu Ile Glu Glu His  Asp Leu Glu Lys Ser  Lys Gly Lys 
    1010                 1015                 1020             


Asn Gly  Ser His Arg Gly Leu  Gly Val Lys Thr Ala  Phe Phe Leu 
    1025                 1030                 1035             


Glu Lys  Tyr Gln Val Asp Glu  Leu Gly Lys Asn Ile  Arg Pro Cys 
    1040                 1045                 1050             


Arg Pro  Thr Lys Arg Gln His  Val Arg 
    1055                 1060         


<210>  553
<211>  1063
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  553

Met Glu Asn His Ile Asn Tyr Arg Leu Gly Leu Asp Ile Gly Ile Ala 
1               5                   10                  15      


Ser Val Gly Trp Ser Val Ile Asn Leu Asp Lys Lys Arg Ile Glu Asp 
            20                  25                  30          


Leu Gly Val Arg Ile Phe Asn Ala Ala Glu His Pro Lys Asp Gly Ser 
        35                  40                  45              


Ser Leu Ala Leu Pro Arg Arg Ile Ala Arg Gly Arg Arg Arg Leu Leu 
    50                  55                  60                  


Arg Arg Lys Ala Tyr Arg Val Lys Arg Val Arg Lys Leu Ile Val Asp 
65                  70                  75                  80  


Tyr Asn Ile Leu Thr Lys Asp Gln Leu Asn Asp Leu Phe Lys Gln Lys 
                85                  90                  95      


Cys Gln Asn Ser Val Trp Glu Ile Arg Val Lys Ala Leu Asp Asn Lys 
            100                 105                 110         


Val Thr Asn Glu Glu Trp Ala Arg Leu Leu Ile Asn Leu Cys Lys Arg 
        115                 120                 125             


Arg Gly Phe Lys Ser Asn Arg Lys Asn Glu Ala Asp Asp Lys Glu Thr 
    130                 135                 140                 


Lys Lys Val Ile Asn Ser Ile Lys Ser Asn Ile Lys Gly Met Lys Glu 
145                 150                 155                 160 


Ser Gly Ser Arg Thr Ile Gly Glu Tyr Ile Tyr Thr Gln Thr Leu Ser 
                165                 170                 175     


Ser Gly Asp Ile Ser Lys Ser Phe Arg Asn Lys Asp Gly Gln Tyr Asn 
            180                 185                 190         


Asn Cys Val Ser Arg Lys Met Ile Glu Glu Glu Ile Lys Ile Leu Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Leu Gly Cys Glu Phe Ala Ser Glu Glu Ile Glu 
    210                 215                 220                 


Glu Lys Tyr Leu Ala Ile Phe Asn Ser Gln Arg Pro Phe Ser Lys Phe 
225                 230                 235                 240 


Glu Asp Leu Glu Lys Met Val Gly Phe Cys Thr Phe Glu Lys Ala Ser 
                245                 250                 255     


Lys Glu Arg Arg Ala Pro Lys Cys Ser Ile Ser Ala Glu Glu Phe Val 
            260                 265                 270         


Leu Tyr Asp Asn Met Asn Lys Leu Ser Ile Leu Asn Lys Gly Asp Thr 
        275                 280                 285             


Arg Lys Leu Thr Asn Glu Glu Arg Lys Ile Ile Val Glu Asn Ala Phe 
    290                 295                 300                 


Lys Asn Lys Glu Ile Lys Tyr Ser Lys Leu Arg Lys Ile Leu Asn Leu 
305                 310                 315                 320 


Ser Asn Asp Glu Arg Phe Ser Thr Leu Ser Tyr Ser Asn Lys Thr Glu 
                325                 330                 335     


Leu Ser Lys Thr Glu Asn Ser Lys Phe Val Ala Leu Lys Gly Tyr His 
            340                 345                 350         


Glu Ile Lys Ser Ala Ile Glu Lys Ser Phe Gly Lys Asp Thr Trp Asn 
        355                 360                 365             


Arg Val Lys Asn Asn Arg Thr Met Leu Asn Asp Ile Ala Tyr Val Leu 
    370                 375                 380                 


Ser Ile Ala Lys Thr Asp Glu Asp Ile Ile Lys Gln Leu Ser Leu Arg 
385                 390                 395                 400 


Asn Ile Pro Arg Glu Ile Ile Asp Ala Val Leu Gly Leu Ser Phe Ser 
                405                 410                 415     


Lys Phe Ser Asn Leu Ser Ile Lys Ala Ile Glu Lys Ile Leu Pro Tyr 
            420                 425                 430         


Leu Lys Asp Gly Gln Gln Tyr Asn Glu Ala Cys Lys Ser Ala Gly Tyr 
        435                 440                 445             


Asp Phe Lys Ala Thr Tyr Asn Gly Glu Lys Ser Tyr Lys Leu Pro Leu 
    450                 455                 460                 


Ile Asp Ile Asp Glu Ile Val Asn Pro Val Val Asn Arg Ala Leu Ser 
465                 470                 475                 480 


Gln Thr Arg Lys Val Ile Asn Ala Val Ile Asp Arg Tyr Gly Ser Pro 
                485                 490                 495     


Ile Gly Ile Asn Ile Glu Leu Ala Arg Asp Leu Ala Lys Asn Phe Lys 
            500                 505                 510         


Asp Arg Ser Lys Ile Lys Lys Glu Gln Glu Glu Asn Arg Ala Asn Lys 
        515                 520                 525             


Glu Lys Ile Lys Asn Glu Leu Lys Met Leu Met Asn Lys Glu Pro Thr 
    530                 535                 540                 


Ser Ser Glu Ile Leu Lys Tyr Arg Leu Trp Lys Glu Gln Arg Glu Glu 
545                 550                 555                 560 


Cys Ala Tyr Ser Gln Glu Arg Ile Tyr Ile Glu Asp Leu Phe Lys Val 
                565                 570                 575     


Gly Gln Tyr Glu Ile Asp His Ile Ile Pro Phe Ser Arg Ser Phe Asp 
            580                 585                 590         


Asp Ser Leu Asn Asn Lys Val Leu Val Lys Ala Ala Glu Asn Gln Arg 
        595                 600                 605             


Lys Ala Asn Arg Ile Pro Tyr Glu Tyr Phe Gly Ser Asp Glu Asp Arg 
    610                 615                 620                 


Trp Tyr Arg Phe Glu Val Trp Val Glu Gln Ser Ser Leu Ser Phe Lys 
625                 630                 635                 640 


Lys Lys Asn Asn Leu Leu Lys Lys Arg Phe Ser Arg Glu Asp Ser Ile 
                645                 650                 655     


Glu Phe Lys Asp Arg Asn Leu Val Asp Thr Gln Tyr Ile Ser Arg Tyr 
            660                 665                 670         


Ile Ser Asn Phe Ile Lys Ser Arg Leu Ile Phe Lys Glu Ser Asp Ser 
        675                 680                 685             


Lys Gln Lys Val Ile Thr Ile Asn Gly Arg Ala Thr Ser Tyr Leu Arg 
    690                 695                 700                 


Ala Lys Trp Gly Leu Leu Lys Val Arg Glu Asn Gly Asp Lys His His 
705                 710                 715                 720 


Ala Leu Asp Ala Thr Val Ile Ala Val Ala Thr Gln Gly Met Val Gln 
                725                 730                 735     


Glu Ile Ser Lys Tyr Ser Lys Ala His Glu Leu Lys Tyr Ile Arg Gln 
            740                 745                 750         


Gly Asp Glu Phe Ile Asp Ile Glu Thr Gly Glu Ile Val Lys Val Lys 
        755                 760                 765             


Asp His Lys His Leu Ser Lys Asp Arg Leu Pro Glu Pro Trp Leu Gly 
    770                 775                 780                 


Phe Ser Ser Glu Leu Arg Leu Arg Leu Ser Glu Asn Pro Ile Glu Glu 
785                 790                 795                 800 


Leu Lys Lys Ser Pro Ile Ser Thr Tyr Asp Glu Glu Phe Ile Arg Lys 
                805                 810                 815     


Thr Val Arg Pro Ile Phe Val Ser Arg Val Pro Tyr Arg Lys Val Thr 
            820                 825                 830         


Gly Arg Leu Phe Glu Glu Thr Val Tyr Ser Pro Lys Ala Phe Ile Asp 
        835                 840                 845             


Glu Gly Phe Ile Ala Lys Lys Lys Leu Gln Asp Leu Thr Leu Lys Asp 
    850                 855                 860                 


Phe Glu Asn Ile Tyr Asn Tyr Asn Ser Asp Lys Lys Leu Tyr Asp Ala 
865                 870                 875                 880 


Leu Arg Glu Arg Leu Glu Glu Tyr Gly Gly Asn Ala Lys Lys Ala Phe 
                885                 890                 895     


Ala Glu Glu Phe Arg Lys Pro Thr Lys Thr Gly Lys Leu Gly Pro Ile 
            900                 905                 910         


Val Lys Ser Ile Lys Val Lys Thr Lys Ile Pro Phe Lys Asp Gly Ile 
        915                 920                 925             


Asp Leu Asn Gly Gly Lys Val Lys Lys Asp Gly Leu Val Arg Ile Asp 
    930                 935                 940                 


Ile Tyr Glu Lys Glu Gly Lys Tyr Tyr Val Val Pro Val Tyr Arg Leu 
945                 950                 955                 960 


Asp Ile Gln Gln Gly Asn Ile Pro Lys Lys Ala Ala Val Ala Asn Lys 
                965                 970                 975     


Glu Glu Lys Asp Trp Ile Leu Met Asp Glu Ser Tyr Asn Phe Lys Phe 
            980                 985                 990         


Ser Leu Tyr Lys Asn Asp Leu Ile  Tyr Ile Lys Tyr Asp  Ser Lys Gly 
        995                 1000                 1005             


Glu Tyr  Phe Gly Tyr Tyr Asp  Gly Phe Asp Arg His  Thr Gly Ala 
    1010                 1015                 1020             


Ile Thr  Leu Glu Arg His Asp  Asn Ser Glu Arg Tyr  Arg Gly Ile 
    1025                 1030                 1035             


Gly Ile  Lys Thr Gly Val Lys  Glu Ile Phe Lys Tyr  Asn Val Asp 
    1040                 1045                 1050             


Val Leu  Gly Arg Tyr Tyr Lys  Val Ala Lys 
    1055                 1060             


<210>  554
<211>  1063
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  554

Met Glu Lys Phe His Tyr Val Leu Gly Leu Asp Leu Gly Ile Ala Ser 
1               5                   10                  15      


Val Gly Trp Ala Ala Ile Glu Ile Asp Lys Glu Thr Glu Thr Ser Ile 
            20                  25                  30          


Gly Leu Leu Asp Cys Gly Val Arg Thr Phe Glu Arg Ala Glu Val Pro 
        35                  40                  45              


Lys Thr Gly Asp Ser Leu Ala Lys Ala Arg Arg Glu Ala Arg Ser Thr 
    50                  55                  60                  


Arg Arg Leu Ile Arg Arg Arg Ser His Arg Leu Leu Arg Leu Lys Arg 
65                  70                  75                  80  


Leu Leu Lys Arg Glu Ile Phe Arg Gln Pro Glu Thr Phe Lys Asp Leu 
                85                  90                  95      


Pro Ile Asn Ala Trp Gln Leu Arg Val Lys Gly Leu Asp Ser Arg Leu 
            100                 105                 110         


Asn Glu Tyr Glu Trp Ala Ala Val Leu Leu His Leu Val Lys His Arg 
        115                 120                 125             


Gly Tyr Leu Ser Gln Arg Lys Ser Glu Met Ser Glu Thr Asp Ser Lys 
    130                 135                 140                 


Ser Glu Met Gly Arg Leu Leu Ala Gly Val Ala Glu Asn His Gln Leu 
145                 150                 155                 160 


Leu Gln Gln Glu Gln Tyr Arg Thr Pro Ala Glu Leu Ala Leu Lys Lys 
                165                 170                 175     


Phe Val Lys His Phe Arg Asn Lys Gly Gly Asp Tyr Ala His Thr Phe 
            180                 185                 190         


Asn Arg Leu Asp Leu Gln Ala Glu Leu His Leu Leu Phe Gln Lys Gln 
        195                 200                 205             


Arg Glu Leu Gly Asn Pro Phe Thr Ser Pro Glu Leu Glu Arg Gln Val 
    210                 215                 220                 


Asp Asp Leu Leu Met Thr Gln Arg Ser Ala Leu Gln Gly Asp Ala Ile 
225                 230                 235                 240 


Leu Lys Met Leu Gly His Cys Gly Phe Glu Pro Glu Gln Phe Lys Ala 
                245                 250                 255     


Ala Lys Asn Thr Phe Ser Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu 
            260                 265                 270         


Asn Asn Leu Arg Ile Gln Asp Gln Gly Lys Glu Arg Ala Leu Thr Ala 
        275                 280                 285             


Asp Glu Arg Thr Lys Leu Leu Asp Glu Pro Tyr Lys Lys Ser Lys Leu 
    290                 295                 300                 


Thr Tyr Ala Gln Val Arg Lys Leu Leu Ser Leu Pro Gln Thr Ala Ile 
305                 310                 315                 320 


Phe Lys Gly Leu Arg Tyr Asp Leu Glu His Asp Lys Lys Ala Glu Asn 
                325                 330                 335     


Ser Thr Leu Met Glu Met Lys Ser Tyr His Asn Ile Arg Gln Thr Leu 
            340                 345                 350         


Glu Lys Ser Gly Leu Lys Thr Glu Trp Gln Ser Ile Ala Thr Gln Pro 
        355                 360                 365             


Glu Ile Leu Asp Ala Ile Gly Thr Ala Phe Ser Ile Tyr Lys Thr Asp 
    370                 375                 380                 


Glu Asp Ile Ser His Glu Leu Lys Thr Cys Arg Leu Pro Glu Asn Val 
385                 390                 395                 400 


Leu Asn Glu Leu Leu Lys Asn Ile Asn Phe Asp Gly Phe Ile Gln Leu 
                405                 410                 415     


Ser Leu Thr Ala Leu Arg Lys Ile Leu Pro Leu Met Glu Gln Gly Tyr 
            420                 425                 430         


Arg Tyr Asp Glu Ala Cys Thr Gln Ile Tyr Gly Asn His His Ser Gly 
        435                 440                 445             


Ser Leu Gln Gln Glu Ser Lys Gln Phe Leu Pro His Ile Pro Ile Asp 
    450                 455                 460                 


Asp Val Arg Asn Pro Val Val Phe Arg Thr Leu Thr Gln Ala Arg Lys 
465                 470                 475                 480 


Val Val Asn Ala Ile Ile Arg Arg Tyr Gly Ser Pro Ala Arg Val His 
                485                 490                 495     


Ile Glu Met Ala Arg Glu Leu Gly Lys Ser Lys Ser Asp Arg Asp Arg 
            500                 505                 510         


Ile Glu Lys Gln Gln Gln Lys Asn Lys Lys Glu Arg Glu Asn Ala Val 
        515                 520                 525             


Ala Lys Phe Lys Glu Asp Phe Pro Asp Phe Val Gly Glu Pro Arg Gly 
    530                 535                 540                 


Lys Asp Ile Leu Lys Met Arg Leu Tyr Glu Gln Gln His Gly Lys Cys 
545                 550                 555                 560 


Leu Tyr Ser Gly His Asp Ile Asp Ile Asn Arg Leu Asn Glu Lys Gly 
                565                 570                 575     


Tyr Val Glu Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp 
            580                 585                 590         


Ser Gln Asn Asn Lys Val Leu Val Leu Gly Ser Glu Asn Gln Asn Lys 
        595                 600                 605             


Arg Asn Gln Thr Pro Asp Glu Tyr Leu Asp Gly Ala Asn Asn Ser Gln 
    610                 615                 620                 


Arg Trp Leu Glu Phe Gln Ala Arg Val Gln Thr Cys His Phe Ser Tyr 
625                 630                 635                 640 


Gly Lys Lys Gln Arg Ile Gln Leu Ala Lys Leu Asp Asp Glu Thr Glu 
                645                 650                 655     


Lys Gly Phe Leu Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ala Arg 
            660                 665                 670         


Phe Met Cys Gln Phe Val Gln Glu Asn Leu Tyr Leu Thr Gly Lys Gly 
        675                 680                 685             


Lys Arg Leu Val Phe Ala Ser Asn Gly Gly Met Thr Ala Thr Leu Arg 
    690                 695                 700                 


Asn Leu Trp Gly Leu Arg Lys Val Arg Glu Asp Asn Asp Arg His His 
705                 710                 715                 720 


Ala Leu Asp Ala Ile Val Val Ala Cys Ser Thr Ala Ser Met Gln Gln 
                725                 730                 735     


Lys Ile Thr Lys Ala Phe Gln Arg His Glu Ser Ile Glu Tyr Val Asp 
            740                 745                 750         


Thr Glu Thr Gly Glu Val Lys Phe Arg Ile Pro Gln Pro Trp Asp Phe 
        755                 760                 765             


Phe Arg Gln Glu Val Met Ile Arg Val Phe Ser Asp Gln Pro Cys Glu 
    770                 775                 780                 


Asp Leu Val Glu Lys Leu Ser Ala Arg Pro Glu Ala Leu His Asp Asn 
785                 790                 795                 800 


Val Thr Pro Leu Phe Val Ser Arg Ala Pro Asn Arg Lys Met Ser Gly 
                805                 810                 815     


Gln Gly His Leu Glu Thr Ile Lys Ser Ala Lys Arg Leu Ser Glu Glu 
            820                 825                 830         


Asn Ser Met Val Lys Lys Pro Leu Thr Thr Leu Lys Leu Lys Asp Ile 
        835                 840                 845             


Pro Glu Ile Val Gly Tyr Pro Ser Arg Glu Pro Gln Leu Tyr Ala Ala 
    850                 855                 860                 


Leu Lys Thr Arg Leu Glu Thr His Asp Asp Asp Pro Ile Lys Ala Phe 
865                 870                 875                 880 


Ala Lys Pro Phe Tyr Lys Pro Asn Lys Asn Gly Glu Leu Gly Ala Leu 
                885                 890                 895     


Val Arg Ser Val Arg Val Lys Gly Val Gln Asn Thr Gly Val Met Val 
            900                 905                 910         


His Asp Gly Lys Gly Ile Ala Asp Asn Ala Thr Met Val Arg Val Asp 
        915                 920                 925             


Val Tyr Thr Lys Ala Gly Lys Asn Tyr Leu Val Pro Val Tyr Val Trp 
    930                 935                 940                 


Gln Val Ala Gln Gly Ile Leu Pro Asn Arg Ala Val Thr Ser Gly Lys 
945                 950                 955                 960 


Ser Glu Ala Asp Trp Asp Leu Ile Asp Glu Ser Phe Glu Phe Lys Phe 
                965                 970                 975     


Ser Leu Ser Arg Gly Asp Leu Val Glu Met Ile Ser Asn Lys Gly Arg 
            980                 985                 990         


Ile Phe Gly Tyr Tyr Asn Gly Leu  Asp Arg Ala Asn Gly  Ser Ile Gly 
        995                 1000                 1005             


Ile Arg  Glu His Asp Leu Glu  Lys Ser Lys Gly Lys  Asp Gly Val 
    1010                 1015                 1020             


His Arg  Val Gly Val Lys Thr  Ala Thr Ala Phe Asn  Lys Tyr His 
    1025                 1030                 1035             


Val Asp  Pro Leu Gly Lys Glu  Ile His Arg Cys Ser  Ser Glu Pro 
    1040                 1045                 1050             


Arg Pro  Thr Leu Lys Ile Lys  Ser Lys Lys 
    1055                 1060             


<210>  555
<211>  1063
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  555

Met Glu Lys Asp Tyr Ile Leu Gly Leu Asp Ile Gly Ile Gly Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Asp Thr Lys Ser Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Ala Asp Asn Asn Leu Gly Arg Arg 
        35                  40                  45              


Ala Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Ser Leu Leu Ser Glu Tyr Lys Ile Ile Ser Gly Leu 
65                  70                  75                  80  


Ala Pro Thr Asn Asn Gln Pro Tyr Asn Ile Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Lys Asp Glu Leu Ala Val Ala Leu Leu His Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile His Asn Val Asp Val Ala Ala Asp Lys Glu Glu 
        115                 120                 125             


Thr Ala Ser Asp Ser Leu Ser Thr Lys Asp Gln Ile Asn Lys Asn Ala 
    130                 135                 140                 


Lys Phe Leu Glu Ser Arg Tyr Val Cys Glu Leu Gln Lys Glu Arg Leu 
145                 150                 155                 160 


Glu Asn Glu Gly His Val Arg Gly Val Glu Asn Arg Phe Leu Thr Lys 
                165                 170                 175     


Asp Ile Val Arg Glu Ala Lys Lys Ile Ile Asp Thr Gln Met Gln Tyr 
            180                 185                 190         


Tyr Pro Glu Ile Asp Glu Thr Phe Lys Glu Lys Tyr Ile Ser Leu Val 
        195                 200                 205             


Glu Thr Arg Arg Glu Tyr Tyr Glu Gly Pro Gly Lys Gly Ser Pro Tyr 
    210                 215                 220                 


Gly Trp Asp Ala Asp Val Lys Lys Trp Tyr Gln Leu Met Met Gly His 
225                 230                 235                 240 


Cys Thr Tyr Phe Pro Val Glu Phe Arg Ser Val Lys Tyr Ala Tyr Thr 
                245                 250                 255     


Ala Asp Leu Tyr Asn Ala Leu Asn Asp Leu Asn Asn Leu Thr Ile Ala 
            260                 265                 270         


Arg Asp Asp Asn Pro Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile 
        275                 280                 285             


Glu Asn Val Phe Lys Gln Lys Arg Asn Pro Thr Leu Lys Gln Ile Ala 
    290                 295                 300                 


Lys Glu Ile Gly Val Asn Asp Ile Asn Ile Ser Gly Tyr Arg Val Thr 
305                 310                 315                 320 


Lys Ser Gly Lys Pro Gln Phe Thr Ser Phe Lys Leu Phe His Asp Leu 
                325                 330                 335     


Lys Lys Val Val Lys Asp His Ala Ile Leu Asp Asp Ile Asp Leu Leu 
            340                 345                 350         


Asn Gln Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile 
        355                 360                 365             


Val Ala Glu Leu Gly Gln Leu Glu Tyr Leu Met Ser Glu Ala Asp Lys 
    370                 375                 380                 


Gln Ser Ile Ser Glu Leu Thr Gly Tyr Thr Gly Thr His Ser Leu Ser 
385                 390                 395                 400 


Leu Lys Cys Met Asn Met Ile Ile Asp Glu Leu Trp His Ser Ser Met 
                405                 410                 415     


Asn Gln Met Glu Val Phe Thr Tyr Leu Asn Met Arg Pro Lys Lys Tyr 
            420                 425                 430         


Glu Leu Lys Gly Tyr Gln Arg Ile Pro Thr Asp Met Ile Asp Asp Ala 
        435                 440                 445             


Ile Leu Ser Pro Val Val Lys Arg Ser Phe Lys Gln Ala Ile Gly Val 
    450                 455                 460                 


Val Asn Ala Ile Ile Lys Lys Tyr Gly Leu Pro Lys Asp Ile Ile Ile 
465                 470                 475                 480 


Glu Leu Ala Arg Glu Ser Asn Ser Ala Glu Lys Ser Arg Tyr Leu Arg 
                485                 490                 495     


Ala Ile Gln Lys Lys Asn Glu Lys Thr Arg Glu Arg Ile Glu Ala Ile 
            500                 505                 510         


Ile Lys Glu Tyr Gly Asn Glu Asn Ala Lys Gly Leu Val Gln Lys Ile 
        515                 520                 525             


Lys Leu His Asp Ala Gln Glu Gly Lys Cys Leu Tyr Ser Leu Lys Asp 
    530                 535                 540                 


Ile Pro Leu Glu Asp Leu Leu Arg Asn Pro Asn Asn Tyr Asp Ile Asp 
545                 550                 555                 560 


His Ile Ile Pro Arg Ser Val Ser Phe Asp Asp Ser Met His Asn Lys 
                565                 570                 575     


Val Leu Val Arg Arg Glu Gln Asn Ala Lys Lys Asn Asn Gln Thr Pro 
            580                 585                 590         


Tyr Gln Tyr Leu Thr Ser Gly Tyr Ala Asp Ile Lys Tyr Ser Val Phe 
        595                 600                 605             


Lys Gln His Val Leu Asn Leu Ala Glu Asn Lys Asp Arg Met Thr Lys 
    610                 615                 620                 


Lys Lys Arg Glu Tyr Leu Leu Glu Glu Arg Asn Ile Asn Lys Tyr Asp 
625                 630                 635                 640 


Val Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Thr 
                645                 650                 655     


Thr Arg Glu Leu Thr Thr Leu Leu Lys Thr Tyr Phe Thr Ile Asn Asn 
            660                 665                 670         


Leu Asp Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Phe Leu 
        675                 680                 685             


Arg Lys Arg Trp Gly Phe Lys Lys Asn Arg Asp Glu Gly Tyr Lys His 
    690                 695                 700                 


His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Tyr Leu Phe Lys 
705                 710                 715                 720 


Glu His Lys Leu Leu Lys Glu Ile Lys Asp Val Ser Asp Leu Ala Gly 
                725                 730                 735     


Asp Glu Arg Asn Ser Asn Val Lys Asp Glu Asp Gln Tyr Glu Glu Val 
            740                 745                 750         


Phe Gly Gly Tyr Phe Lys Ile Glu Asp Ile Lys Lys Tyr Lys Ile Lys 
        755                 760                 765             


Lys Phe Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile Asn 
    770                 775                 780                 


Asp Thr Ile Tyr Ser Thr Arg Val Lys Asp Asp Lys Arg Tyr Leu Ile 
785                 790                 795                 800 


Asn Thr Leu Lys Asn Leu Tyr Asp Lys Ser Asn Gly Asp Leu Lys Glu 
                805                 810                 815     


Arg Met Gln Lys Asp Pro Glu Ser Leu Leu Met Tyr His His Asp Pro 
            820                 825                 830         


Gln Thr Phe Glu Lys Leu Lys Ile Val Met Ser Gln Tyr Glu Asn Glu 
        835                 840                 845             


Lys Asn Pro Leu Ala Lys Tyr Phe Glu Glu Thr Gly Gln Tyr Leu Thr 
    850                 855                 860                 


Lys Tyr Ala Lys His Asp Asn Gly Pro Ala Ile His Lys Ile Lys Tyr 
865                 870                 875                 880 


Tyr Gly Asn Lys Leu Val Glu His Leu Asp Ile Thr Lys Asn Tyr His 
                885                 890                 895     


Asn Pro Gln Asn Lys Val Val Gln Leu Ser Gln Lys Ser Phe Arg Phe 
            900                 905                 910         


Asp Val Tyr Gln Thr Asp Lys Gly Tyr Lys Phe Ile Ser Ile Ala Tyr 
        915                 920                 925             


Leu Thr Leu Lys Asn Glu Lys Asn Tyr Tyr Ala Ile Ser Gln Glu Lys 
    930                 935                 940                 


Tyr Asp Gln Leu Lys Ser Glu Lys Lys Ile Ser Asn Asn Ala Val Phe 
945                 950                 955                 960 


Ile Gly Ser Phe Tyr Thr Ser Asp Ile Ile Glu Ile Asn Asn Glu Lys 
                965                 970                 975     


Phe Arg Val Ile Gly Val Asn Ser Asp Lys Asn Asn Leu Ile Glu Val 
            980                 985                 990         


Asp Arg Ile Asp Ile Arg Gln Lys  Glu Phe Ile Glu Leu  Glu Glu Glu 
        995                 1000                 1005             


Lys Gln  Val Asn Tyr Met Asn  Ser Asp Ser Tyr Leu  Lys Asn Asn 
    1010                 1015                 1020             


Arg Ile  Lys Val Thr Ile Gly  Arg Lys Thr Thr Asn  Ile Glu Lys 
    1025                 1030                 1035             


Phe His  Thr Asp Ile Leu Gly  Asn Met Tyr Lys Ser  Lys Arg Pro 
    1040                 1045                 1050             


Lys Ala  Pro Gln Leu Val Phe  Lys Lys Gly 
    1055                 1060             


<210>  556
<211>  1064
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  556

Met Ser Glu Cys Val Thr Arg Arg Ile Leu Gly Ile Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Cys Gly Trp Gly Val Ile Glu Val Gly Glu Ala Ser Gly Ser 
            20                  25                  30          


Ile Ile Ala Ser Gly Val Arg Cys Phe Asp Ala Pro Leu Ile Asp Lys 
        35                  40                  45              


Thr Gly Glu Pro Lys Ser Ala Thr Arg Arg Thr Ala Arg Gly Gln Arg 
    50                  55                  60                  


Arg Ile Ile Arg Arg Arg Arg Gln Arg Met Asn Ala Val Arg Arg Leu 
65                  70                  75                  80  


Leu Ala Glu Phe Gly Val Leu Thr Gly Arg Ser Pro Asp Ala Leu His 
                85                  90                  95      


Gln Ala Leu Leu Arg Leu Ser Gln Ser Val Ala Gly Ser Gln Val Thr 
            100                 105                 110         


Pro Trp Thr Leu Arg Ala Ala Ala His Glu Arg Lys Leu Thr Asn Asp 
        115                 120                 125             


Glu Leu Ala Val Val Leu Gly His Ile Ala Arg His Arg Gly Phe Arg 
    130                 135                 140                 


Ser Asn Ser Lys Asn Asp Gly Gly Ala Asn Ala Ala Asp Glu Thr Ser 
145                 150                 155                 160 


Lys Met Lys Lys Ala Met Glu Thr Thr Arg Glu Gly Leu Ala Arg Tyr 
                165                 170                 175     


His Ser Phe Gly Ala Met Ile Ala Ser Asp Pro Lys Phe Ala Asp Arg 
            180                 185                 190         


Lys Arg Asn Arg Asp Lys Asp Tyr Ser His Thr Ala Lys Arg Ser Asp 
        195                 200                 205             


Leu Glu Asp Glu Val Arg Thr Ile Phe Arg Ser Gln Thr Arg Phe Gly 
    210                 215                 220                 


Ser Leu Val Ala Ser Glu Lys Leu Ser Gln Ala Phe Ala Asp Ala Ala 
225                 230                 235                 240 


Phe Phe Gln Arg Pro Leu Gln Asp Ser Glu Asp Met Val Gly Ser Cys 
                245                 250                 255     


Pro Phe Glu Pro Gly Gln Lys Arg Thr Ala Arg Arg Ala Pro Ser Phe 
            260                 265                 270         


Glu Leu Phe Arg Phe Leu Ser Arg Leu Ala Asn Leu Lys Leu Thr Val 
        275                 280                 285             


Gly Arg Ala Pro Glu Arg Arg Leu Thr Pro Asp Glu Ile Ala Leu Ala 
    290                 295                 300                 


Ala Lys Gly Phe Gly Glu Thr Lys Lys Ser Ile Thr Phe Lys Ser Leu 
305                 310                 315                 320 


Arg Glu Ala Leu Asp Leu Asp Pro Asn Ala Arg Phe Ser Gly Val Ala 
                325                 330                 335     


Lys Glu Lys Glu Ser Thr Leu Asp Val Ala Ala Arg Thr Gly Gly Ala 
            340                 345                 350         


Ala Tyr Gly Thr Lys Thr Leu Lys Asp Ala Leu Gly Asp Ala Pro Trp 
        355                 360                 365             


Arg Ser Leu Ser Arg Met Pro Glu Lys Leu Asp Arg Ile Ala Glu Ile 
    370                 375                 380                 


Leu Ser Phe Arg Glu Asp Met Lys Ala Ile Arg Asn Gly Leu Glu Glu 
385                 390                 395                 400 


Val Gly Leu Asp Gly Leu Val Val Asp Ala Leu Met Gln Ala Thr Ala 
                405                 410                 415     


Asn Gly Asp Phe Lys Asp Phe Thr Arg Ala Ala His Ile Ser Ala Leu 
            420                 425                 430         


Ala Ala Arg Asn Ile Ile Pro Gly Leu Arg Glu Gly Leu Val Tyr Ser 
        435                 440                 445             


Asp Ala Cys Thr Arg Val Gly Tyr Asp His Ala Ala Arg Pro Ala Val 
    450                 455                 460                 


Pro Leu Ser Gln Ile Gly Ser Pro Val Thr Arg Lys Ala Leu Ser Glu 
465                 470                 475                 480 


Ala Leu Lys Gln Val Arg Ala Val Ala Arg Glu Tyr Gly Pro Ile Asp 
                485                 490                 495     


Tyr Phe His Ile Glu Leu Ala Arg Ser Ile Gly Lys Ser Ala Glu Glu 
            500                 505                 510         


Arg Lys Lys Leu Thr Asp Gly Ile Glu Ala Arg Asn Val Glu Lys Glu 
        515                 520                 525             


Lys Arg Arg Lys Glu Ala Ala Glu His Leu Gly Arg Ala Pro Ser Asp 
    530                 535                 540                 


Asp Glu Leu Leu Arg Tyr Glu Leu Ala Lys Glu Gln Asn Phe Lys Cys 
545                 550                 555                 560 


Ile Tyr Ser Gly Asp Pro Ile Asp Pro Ala Gly Ile Ser Ala Asn Asp 
                565                 570                 575     


Thr Arg Tyr Gln Val Asp His Ile Leu Pro Trp Ser Arg Phe Gly Asp 
            580                 585                 590         


Asp Ser Tyr Val Asn Lys Thr Leu Cys Thr Ala Arg Ser Asn Gln Asn 
        595                 600                 605             


Lys Arg Gly Arg Thr Pro Phe Glu Trp Phe Asp Ala Asp Lys Thr Glu 
    610                 615                 620                 


Ala Glu Trp Met Glu Tyr Ser Ala Arg Val Glu Asp Leu Lys Glu Val 
625                 630                 635                 640 


Lys Gly Arg Lys Lys Arg Asn Tyr Ser Ile Lys Asp Ala Ala Ser Val 
                645                 650                 655     


Glu Asp Lys Phe Lys Ala Arg Asn Leu Thr Asp Thr Gln Trp Ala Thr 
            660                 665                 670         


Arg Leu Leu Ala Asp Glu Leu Lys Arg Met Phe Pro Pro Arg Glu Cys 
        675                 680                 685             


Glu Arg Val Val Thr Val Arg Ala Asp Gly Gly Asn Asp Gly Leu Ser 
    690                 695                 700                 


Ile Val Glu Glu Arg Arg Val Phe Thr Arg Pro Gly Ala Ile Thr Ser 
705                 710                 715                 720 


Lys Leu Arg Arg Ala Trp Gly Leu Glu Gly Leu Lys Lys Gln Asp Gly 
                725                 730                 735     


Lys Arg Val Glu Asp Asp Arg His His Ala Val Asp Ala Leu Val Leu 
            740                 745                 750         


Ala Ala Thr Thr Glu Ser Leu Leu Asn Arg Leu Thr Val Glu Val Gln 
        755                 760                 765             


Gln Arg Glu Arg Glu Gly Arg Gln Asp Asp Ile Phe His Cys Ser Gln 
    770                 775                 780                 


Pro Trp Pro Gly Phe Arg Val Asp Val Gln Arg Thr Val Tyr Gly Ser 
785                 790                 795                 800 


Glu Thr Met Pro Gly Ile Phe Val Ser Arg Ala Glu Arg Arg Arg Ala 
                805                 810                 815     


Arg Gly Lys Ala His Asp Ala Thr Val Lys Gln Ile Arg Asp Ile Asp 
            820                 825                 830         


Gly Glu Arg Ile Val Phe Glu Arg Lys Pro Ile Glu Lys Leu Thr Asp 
        835                 840                 845             


Lys Asp Leu Glu Arg Ile Pro Val Pro Glu Pro Tyr Gly Lys Ala Ala 
    850                 855                 860                 


Asp Pro Lys Lys Leu Arg Asp Glu Leu Val Glu Asn Leu Arg Ala Trp 
865                 870                 875                 880 


Ile Ala Ala Gly Lys Pro Lys Asp Lys Pro Pro Arg Ser Pro Lys Gly 
                885                 890                 895     


Asp Ile Ile Arg Lys Val Arg Ile Glu Thr Lys Asp Lys Val Ala Val 
            900                 905                 910         


Glu Ile Asn Gly Gly Thr Val Asp Arg Gly Asp Met Ala Arg Val Asp 
        915                 920                 925             


Val Phe Arg Lys Lys Asn Lys Lys Gly Val Trp Glu Phe Tyr Val Ile 
    930                 935                 940                 


Pro Ile Tyr Pro His Gln Ile Val Ala Ser Ala Leu Pro Pro Asn Arg 
945                 950                 955                 960 


Ala Val Ile Ala Tyr Lys Ala Glu Ser Glu Trp Thr Ala Ile Asp Gly 
                965                 970                 975     


Cys Phe Glu Phe Ala Trp Ser Leu Asn Pro Met Ser Tyr Leu Glu Leu 
            980                 985                 990         


Val Lys Ser Asn Gly Glu Leu Ile  Glu Gly Tyr Phe Arg  Ser Met Asp 
        995                 1000                 1005             


Arg Thr  Thr Gly Ala Ile Asn  Leu Ser Pro Met Ser  Thr Asn Ser 
    1010                 1015                 1020             


Glu Thr  Ile Arg Ser Ile Gly  Val Lys Thr Leu Ser  Ser Phe Arg 
    1025                 1030                 1035             


Lys Phe  Thr Val Asp Arg Leu  Gly Arg Lys Phe Glu  Ile Pro Arg 
    1040                 1045                 1050             


Glu Val  Arg Thr Trp Arg Gly  Glu Ala Cys Thr 
    1055                 1060                 


<210>  557
<211>  1064
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  557

Met Lys Arg Thr Ser Leu Arg Ala Tyr Arg Leu Gly Val Asp Leu Gly 
1               5                   10                  15      


Ala Asn Ser Leu Gly Trp Phe Val Val Trp Leu Asp Asp His Gly Gln 
            20                  25                  30          


Pro Glu Gly Leu Gly Pro Gly Gly Val Arg Ile Phe Pro Asp Gly Arg 
        35                  40                  45              


Asn Pro Gln Ser Lys Gln Ser Asn Ala Ala Gly Arg Arg Leu Ala Arg 
    50                  55                  60                  


Ser Ala Arg Arg Arg Arg Asp Arg Tyr Leu Gln Arg Arg Gly Lys Leu 
65                  70                  75                  80  


Met Gly Leu Leu Val Lys His Gly Leu Met Pro Ala Asp Glu Pro Ala 
                85                  90                  95      


Arg Lys Arg Leu Glu Cys Leu Asp Pro Tyr Gly Leu Arg Ala Lys Ala 
            100                 105                 110         


Leu Asp Glu Val Leu Pro Leu His His Val Gly Arg Ala Leu Phe His 
        115                 120                 125             


Leu Asn Gln Arg Arg Gly Leu Phe Ala Asn Arg Ala Ile Glu Gln Gly 
    130                 135                 140                 


Asp Lys Asp Ala Ser Ala Ile Lys Ala Ala Ala Gly Arg Leu Gln Thr 
145                 150                 155                 160 


Ser Met Gln Ala Cys Gly Ala Arg Thr Leu Gly Glu Phe Leu Asn Arg 
                165                 170                 175     


Arg His Gln Leu Arg Ala Thr Val Arg Ala Arg Ser Pro Val Gly Gly 
            180                 185                 190         


Asp Val Gln Ala Arg Tyr Glu Phe Tyr Pro Thr Arg Ala Met Val Asp 
        195                 200                 205             


Ala Glu Phe Glu Ala Ile Trp Ala Ala Gln Ala Pro His His Pro Thr 
    210                 215                 220                 


Met Thr Ala Glu Ala His Asp Thr Ile Arg Glu Ala Ile Phe Ser Gln 
225                 230                 235                 240 


Arg Ala Met Lys Arg Pro Ser Ile Gly Lys Cys Ser Leu Asp Pro Ala 
                245                 250                 255     


Thr Ser Gln Asp Asp Val Asp Gly Phe Arg Cys Ala Trp Ser His Pro 
            260                 265                 270         


Leu Ala Gln Arg Phe Arg Ile Trp Gln Asp Val Arg Asn Leu Ala Val 
        275                 280                 285             


Val Glu Thr Gly Pro Thr Ser Ser Arg Leu Gly Lys Glu Asp Gln Asp 
    290                 295                 300                 


Lys Val Ala Arg Ala Leu Leu Gln Thr Asp Gln Leu Ser Phe Asp Glu 
305                 310                 315                 320 


Ile Arg Gly Leu Leu Gly Leu Pro Ser Asp Ala Arg Phe Asn Leu Glu 
                325                 330                 335     


Ser Asp Arg Arg Asp His Leu Lys Gly Asp Ala Thr Gly Ala Ile Leu 
            340                 345                 350         


Ser Ala Arg Arg His Phe Gly Pro Ala Trp His Asp Arg Ser Leu Asp 
        355                 360                 365             


Arg Gln Ile Asp Ile Val Ala Leu Leu Glu Ser Ala Leu Asp Glu Ala 
    370                 375                 380                 


Ala Ile Ile Ala Ser Leu Gly Thr Thr His Ser Leu Asp Glu Ala Ala 
385                 390                 395                 400 


Ala Gln Arg Ala Leu Ser Ala Leu Leu Pro Asp Gly Tyr Cys Arg Leu 
                405                 410                 415     


Gly Leu Arg Ala Ile Lys Arg Val Leu Pro Leu Met Glu Ala Gly Arg 
            420                 425                 430         


Thr Tyr Ala Glu Ala Ala Ser Ala Ala Gly Tyr Asp His Ala Leu Leu 
        435                 440                 445             


Pro Gly Gly Lys Leu Ser Pro Thr Gly Tyr Leu Pro Tyr Tyr Gly Gln 
    450                 455                 460                 


Trp Leu Gln Asn Asp Val Val Gly Ser Asp Asp Glu Arg Asp Thr Asn 
465                 470                 475                 480 


Glu Arg Arg Trp Gly Arg Leu Pro Asn Pro Thr Val His Ile Gly Ile 
                485                 490                 495     


Gly Gln Leu Arg Arg Val Val Asn Glu Leu Ile Arg Trp His Gly Pro 
            500                 505                 510         


Pro Ala Glu Ile Thr Val Glu Leu Thr Arg Asp Leu Lys Leu Ser Pro 
        515                 520                 525             


Arg Arg Leu Ala Glu Leu Glu Arg Glu Gln Ala Glu Asn Gln Arg Lys 
    530                 535                 540                 


Asn Asp Lys Arg Thr Ser Leu Leu Arg Lys Leu Gly Leu Pro Ala Ser 
545                 550                 555                 560 


Thr His Asn Leu Leu Lys Leu Arg Leu Trp Asp Glu Gln Gly Asp Val 
                565                 570                 575     


Ala Ser Glu Cys Pro Tyr Thr Gly Glu Ala Ile Gly Leu Glu Arg Leu 
            580                 585                 590         


Val Ser Asp Asp Val Asp Ile Asp His Leu Ile Pro Phe Ser Ile Ser 
        595                 600                 605             


Trp Asp Asp Ser Ala Ala Asn Lys Val Val Cys Met Arg Tyr Ala Asn 
    610                 615                 620                 


Arg Glu Lys Gly Asn Arg Thr Pro Phe Glu Ala Phe Gly His Arg Gln 
625                 630                 635                 640 


Gly Arg Pro Tyr Asp Trp Ala Asp Ile Ala Glu Arg Ala Ala Arg Leu 
                645                 650                 655     


Pro Arg Gly Lys Arg Trp Arg Phe Gly Pro Gly Ala Arg Ala Gln Phe 
            660                 665                 670         


Glu Glu Leu Gly Asp Phe Gln Ala Arg Leu Leu Asn Glu Thr Ser Trp 
        675                 680                 685             


Leu Ala Arg Val Ala Lys Gln Tyr Leu Ala Ala Val Thr His Pro His 
    690                 695                 700                 


Arg Ile His Val Leu Pro Gly Arg Leu Thr Ala Leu Leu Arg Ala Thr 
705                 710                 715                 720 


Trp Glu Leu Asn Asp Leu Leu Pro Gly Ser Asp Asp Arg Ala Ala Lys 
                725                 730                 735     


Ser Arg Lys Asp His Arg His His Ala Ile Asp Ala Leu Val Ala Ala 
            740                 745                 750         


Leu Thr Asp Gln Ala Leu Leu Arg Arg Met Ala Asn Ala His Asp Asp 
        755                 760                 765             


Thr Arg Arg Lys Ile Glu Val Leu Leu Pro Trp Pro Thr Phe Arg Ile 
    770                 775                 780                 


Asp Leu Glu Thr Arg Leu Lys Ala Met Leu Val Ser His Lys Pro Asp 
785                 790                 795                 800 


His Gly Leu Gln Ala Arg Leu His Glu Asp Thr Ala Tyr Gly Thr Val 
                805                 810                 815     


Glu His Pro Glu Thr Glu Asp Gly Ala Asn Leu Val Tyr Arg Lys Thr 
            820                 825                 830         


Phe Val Asp Ile Ser Glu Lys Glu Ile Asp Arg Ile Arg Asp Arg Arg 
        835                 840                 845             


Leu Arg Asp Leu Val Arg Ala His Val Ala Gly Glu Arg Gln Gln Gly 
    850                 855                 860                 


Lys Thr Leu Lys Ala Ala Val Leu Ser Phe Ala Gln Arg Arg Asp Ile 
865                 870                 875                 880 


Ala Gly His Pro Asn Gly Ile Arg His Val Arg Leu Thr Lys Ser Ile 
                885                 890                 895     


Lys Pro Asp Tyr Leu Val Pro Ile Arg Asp Lys Ala Gly Arg Ile Tyr 
            900                 905                 910         


Lys Ser Tyr Asn Ala Gly Glu Asn Ala Phe Val Asp Ile Leu Gln Ala 
        915                 920                 925             


Glu Ser Gly Arg Trp Ile Ala Arg Ala Thr Thr Val Phe Gln Ala Asn 
    930                 935                 940                 


Gln Ala Asn Glu Ser His Asp Ala Pro Ala Ala Gln Pro Ile Met Arg 
945                 950                 955                 960 


Val Phe Lys Gly Asp Met Leu Arg Ile Asp His Ala Gly Ala Glu Lys 
                965                 970                 975     


Phe Val Lys Ile Val Arg Leu Ser Pro Ser Asn Asn Leu Leu Tyr Leu 
            980                 985                 990         


Val Glu His His Gln Ala Gly Val  Phe Gln Thr Arg His  Asp Asp Pro 
        995                 1000                 1005             


Glu Asp  Ser Phe Arg Trp Leu  Phe Ala Ser Phe Asp  Lys Leu Arg 
    1010                 1015                 1020             


Glu Trp  Asn Ala Glu Leu Val  Arg Ile Asp Thr Leu  Gly Gln Pro 
    1025                 1030                 1035             


Trp Arg  Arg Lys Arg Gly Leu  Glu Thr Gly Ser Glu  Asp Ala Thr 
    1040                 1045                 1050             


Arg Ile  Gly Trp Thr Arg Pro  Lys Lys Trp Pro 
    1055                 1060                 


<210>  558
<211>  1065
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  558

Met Asn Lys Asp Ile Asn Tyr Ala Leu Gly Leu Asp Ile Gly Ile Thr 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Ile Asn Leu Asp Leu Asn Arg Ile Glu Asp 
            20                  25                  30          


Leu Gly Val Arg Ile Phe Asn Ala Ala Glu Asn Leu Lys Asp Gly Ser 
        35                  40                  45              


Ser Leu Ala Leu Pro Arg Arg Leu Ala Arg Gly Arg Arg Arg Leu Leu 
    50                  55                  60                  


Arg Arg Lys Ala Tyr Arg Val Glu Arg Val Arg Lys Leu Ile Leu Lys 
65                  70                  75                  80  


Asn Asn Ile Leu Thr Lys Glu Glu Leu Glu Gly Leu Phe Lys Asn Lys 
                85                  90                  95      


Glu Val Ile Asp Val Trp Glu Ala Arg Val Lys Gly Leu Asp Tyr Lys 
            100                 105                 110         


Leu Ser Arg Glu Glu Trp Ala Lys Ile Leu Ile Asn Phe Cys Lys Arg 
        115                 120                 125             


Arg Gly Phe Lys Ser Asn Arg Lys Asn Glu Ala Lys Asp Lys Glu Ala 
    130                 135                 140                 


Gly Gln Ile Leu Ser Ser Ile Ser Tyr Asn Ile Glu Lys Met Lys Glu 
145                 150                 155                 160 


Thr Asn Ser Arg Thr Ile Gly Glu Tyr Ile Tyr Asn Glu Val Lys Asn 
                165                 170                 175     


Ser Glu Asp Ser Tyr Ala Pro Leu Arg Asn Lys Phe Gly Glu Tyr Lys 
            180                 185                 190         


Met Cys Val Ser Arg Asp Val Ile Arg Glu Glu Ile His Thr Leu Phe 
        195                 200                 205             


Glu Lys Gln Arg Glu Leu Gly Asn Asn Phe Ala Ser Asp Glu Ile Glu 
    210                 215                 220                 


Asp Lys Tyr Phe Glu Ile Phe Asn Ser Gln Arg Pro Phe Ser Asn Phe 
225                 230                 235                 240 


Glu Asp Leu Glu Lys Leu Val Gly Phe Cys Thr Phe Glu Arg Lys Lys 
                245                 250                 255     


His Lys Arg Ala Pro Lys His Cys Ile Ser Ser Glu Glu Phe Thr Leu 
            260                 265                 270         


Tyr Glu Gly Ile Asn Lys Leu Ser Ile Ile Lys Asp Gly Glu Lys Arg 
        275                 280                 285             


Lys Leu Ser Glu Glu Glu Arg Thr Leu Val Val Asn Glu Ala Phe Asn 
    290                 295                 300                 


Lys Lys Glu Ile Lys Tyr Thr Thr Leu Arg Lys Leu Leu Gly Leu Ser 
305                 310                 315                 320 


Glu Glu Glu Leu Phe Ser Thr Leu Thr Tyr Ser Ile Asp Lys Asp Ile 
                325                 330                 335     


Ser Lys Thr Glu Asn Thr Lys Phe Val Ser Leu Lys Gly Tyr His Glu 
            340                 345                 350         


Ile Lys Arg Ala Ile Glu Lys Gly Val Ser Lys Lys Ala Trp Gln Asp 
        355                 360                 365             


Ile Glu Asn Asn Arg Asn Leu Leu Asn Asp Ile Ala Tyr Val Leu Thr 
    370                 375                 380                 


Leu Gly Lys Thr Asp Asp Glu Ile Glu Lys Gln Leu Arg Leu Arg Asn 
385                 390                 395                 400 


Val Pro Glu Glu Leu Phe Glu Ser Leu Leu Asp Met Ser Phe Asn Lys 
                405                 410                 415     


Phe Asn Asn Leu Ser Ile Tyr Ala Leu Glu Lys Ile Leu Pro Phe Met 
            420                 425                 430         


Lys Glu Gly Tyr Gln Tyr Asn Glu Ala Cys Glu Lys Ala Gly Tyr Asn 
        435                 440                 445             


Phe Lys Ala Ile Tyr Glu Gly Val Arg Thr Lys Lys Leu Pro Val Ile 
    450                 455                 460                 


Glu Ile Asp Glu Ile Val Asn Pro Val Val Asn Arg Ala Leu Ala Gln 
465                 470                 475                 480 


Thr Arg Lys Val Ile Asn Ser Val Ile Asp Lys Tyr Gly Ser Pro Val 
                485                 490                 495     


Arg Ile Asn Ile Glu Leu Ala Arg Asp Leu Ala Lys Asn Phe Lys Asp 
            500                 505                 510         


Arg Lys Leu Ile Glu Lys Glu Gln Lys Glu Asn Arg Ala Asn Leu Asp 
        515                 520                 525             


Lys Ile Arg Glu Asn Leu Arg Glu Leu Met Asp Lys Glu Pro Thr Val 
    530                 535                 540                 


Val Glu Val Leu Lys Tyr Arg Leu Trp Glu Gln Gln Arg Gly Glu Cys 
545                 550                 555                 560 


Ala Tyr Thr Gln Ser Gln Ile Pro Leu Glu Arg Leu Phe Ser Pro Gly 
                565                 570                 575     


Tyr Cys Glu Ile Asp His Ile Ile Pro Phe Ser Arg Ser Phe Asp Asp 
            580                 585                 590         


Ser Leu Ser Asn Lys Ile Leu Val Leu Gly Ser Glu Asn Gln Arg Lys 
        595                 600                 605             


Gly Asn Arg Thr Pro Tyr Glu Tyr Phe Gly Glu Asp Ser Glu Arg Trp 
    610                 615                 620                 


Asn Ser Phe Glu Ile Trp Val Lys Gly Ser Tyr Leu Asn Tyr Lys Lys 
625                 630                 635                 640 


Lys Ala Asn Leu Leu Lys Lys Lys Phe Ser Glu Glu Glu Gln Arg Asp 
                645                 650                 655     


Trp Lys Ala Arg Asn Leu Gln Asp Thr Lys Tyr Ile Cys Arg Tyr Ile 
            660                 665                 670         


Ser Asn Phe Val Asn Asn Arg Leu Glu Phe Lys Glu Ser Glu Asn Lys 
        675                 680                 685             


Gln Lys Val Ile Thr Val Asn Gly Arg Ala Thr Ala Tyr Leu Arg Ser 
    690                 695                 700                 


Arg Trp Gly Leu Asn Lys Val Arg Glu Asp Gly Asp Lys His His Ala 
705                 710                 715                 720 


Leu Asp Ala Ala Val Val Gly Val Thr Thr Gln Gly Met Val Gln Lys 
                725                 730                 735     


Ile Ser Lys Tyr Ser Lys Ala His Glu Leu Tyr Thr Val Arg Lys Asn 
            740                 745                 750         


Asp Glu Phe Val Asp Val Glu Thr Gly Glu Cys Val Asp Val Asp Glu 
        755                 760                 765             


Tyr Lys Glu Ile Arg Lys Asp Ile Leu Pro Arg Pro Trp Arg Gly Phe 
    770                 775                 780                 


Ser Glu Glu Leu Lys Leu Arg Leu Ser Asp Asn Pro Leu Glu Glu Leu 
785                 790                 795                 800 


Lys Lys Ser Pro Ile Glu Thr Tyr Asn Glu Glu Phe Ile Lys Glu Asn 
                805                 810                 815     


Val Lys Pro Ile Phe Val Ser Arg Val Pro Phe Arg Lys Ile Ser Gly 
            820                 825                 830         


Lys Leu Phe Lys Glu Thr Ile Tyr Ser Glu Arg Ala Phe Lys Asp Gly 
        835                 840                 845             


Tyr Phe Val Ser Lys Lys Ser Leu Val Asp Leu Lys Arg Gly Asp Leu 
    850                 855                 860                 


Asp Asn Phe Tyr Asn Tyr Glu Cys Asp Lys Lys Leu Tyr Asp Ala Ile 
865                 870                 875                 880 


Ser Glu Arg Met Lys Glu Phe Asn Tyr Asn Gly Lys Lys Ala Phe Glu 
                885                 890                 895     


Ser Pro Phe Arg Lys Pro Thr Lys Ser Gly Lys Glu Gly Pro Val Val 
            900                 905                 910         


Arg Ser Val Lys Ile Lys Ser Ala Val Pro Phe Lys Asp Gly Ile Pro 
        915                 920                 925             


Met Asn Glu Gly Leu Val Ala Lys Glu Gly Met Val Arg Ile Asp Ile 
    930                 935                 940                 


Tyr Glu Lys Glu Gly Lys Tyr Phe Val Val Pro Val Tyr Arg Tyr Gln 
945                 950                 955                 960 


Leu Ala Lys Gly Ile Val Pro Lys Arg Ala Ala Ile Ala Lys Lys Asp 
                965                 970                 975     


Glu Leu Lys Trp Pro Ile Met Asp Gln Thr Tyr Asn Phe Lys Phe Ser 
            980                 985                 990         


Ile Tyr Lys Asn Asp Leu Ile Glu  Val Lys Tyr Asp Lys  Lys Gly Val 
        995                 1000                 1005             


Tyr Phe  Gly Tyr Tyr Asp Gly  Phe Asp Arg Asn Ser  Ala Ser Leu 
    1010                 1015                 1020             


Thr Ile  Glu Lys His Asp Asn  Ser Glu Arg Tyr Arg  Ser Ile Gly 
    1025                 1030                 1035             


Leu Lys  Val Gly Val Leu Glu  Ile Asn Lys Tyr Glu  Val Asp Val 
    1040                 1045                 1050             


Leu Gly  Asn Tyr His Lys Val  Lys Leu Gly Gly Asn  
    1055                 1060                 1065 


<210>  559
<211>  1065
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  559

Met Asn Lys Asp Ile Asn Tyr Ala Leu Gly Leu Asp Ile Gly Ile Thr 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Ile Asn Leu Asp Leu Asn Arg Ile Glu Asp 
            20                  25                  30          


Leu Gly Val Arg Ile Phe Asn Ala Ala Glu Asn Pro Lys Asp Gly Ser 
        35                  40                  45              


Ser Leu Ala Leu Pro Arg Arg Leu Ala Arg Gly Arg Arg Arg Leu Leu 
    50                  55                  60                  


Arg Arg Lys Ala Tyr Arg Val Glu Arg Val Arg Lys Leu Ile Val Lys 
65                  70                  75                  80  


Asn Asn Ile Leu Thr Lys Glu Glu Leu Glu Gly Leu Phe Lys Asn Lys 
                85                  90                  95      


Glu Val Ile Asp Val Trp Glu Ala Arg Val Lys Gly Leu Asp Tyr Lys 
            100                 105                 110         


Leu Ser Arg Glu Glu Trp Ala Lys Ile Leu Ile Asn Phe Cys Lys Arg 
        115                 120                 125             


Arg Gly Phe Lys Ser Asn Arg Lys Asn Glu Thr Lys Asp Lys Glu Ala 
    130                 135                 140                 


Gly Gln Ile Leu Ser Ser Ile Ser Tyr Asn Ile Glu Lys Met Lys Glu 
145                 150                 155                 160 


Thr Asn Ser Arg Thr Ile Gly Glu Tyr Ile Tyr Asn Glu Val Lys Asn 
                165                 170                 175     


Ser Asp Asp Ser Tyr Ala Pro Leu Arg Asn Lys Phe Gly Glu Tyr Lys 
            180                 185                 190         


Met Cys Val Ser Arg Asp Val Leu Arg Glu Glu Ile His Thr Leu Phe 
        195                 200                 205             


Glu Lys Gln Arg Glu Leu Gly Asn Asn Phe Ala Ser Asp Glu Ile Glu 
    210                 215                 220                 


Asp Lys Tyr Phe Glu Ile Phe Asn Ser Gln Arg Pro Phe Ser Asn Phe 
225                 230                 235                 240 


Glu Asp Leu Glu Lys Leu Val Gly Phe Cys Thr Phe Glu Arg Lys Lys 
                245                 250                 255     


His Lys Arg Ala Pro Lys His Cys Ile Ser Ser Glu Glu Phe Thr Leu 
            260                 265                 270         


Tyr Glu Gly Ile Asn Lys Leu Ser Ile Ile Lys Asp Gly Glu Lys Arg 
        275                 280                 285             


Lys Leu Ser Glu Glu Glu Arg Thr Leu Val Val Asn Glu Ala Phe Asn 
    290                 295                 300                 


Lys Lys Glu Ile Lys Tyr Thr Thr Leu Arg Lys Leu Leu Gly Leu Ser 
305                 310                 315                 320 


Glu Asp Glu Leu Phe Ser Thr Leu Thr Tyr Ser Ile Asp Lys Asp Ile 
                325                 330                 335     


Ser Lys Thr Glu Asn Thr Lys Phe Val Ser Leu Lys Gly Tyr His Glu 
            340                 345                 350         


Ile Lys Arg Ala Ile Glu Lys Gly Val Ser Lys Lys Ala Trp Gln Asp 
        355                 360                 365             


Ile Glu Asn Asn Arg Asn Leu Leu Asn Asp Ile Ala Tyr Val Leu Thr 
    370                 375                 380                 


Leu Gly Lys Thr Asp Asp Glu Ile Glu Lys Gln Leu Arg Leu Arg Asn 
385                 390                 395                 400 


Val Pro Glu Glu Leu Phe Glu Ser Leu Leu Asp Met Ser Phe Ser Lys 
                405                 410                 415     


Phe Asn Asn Leu Ser Ile Tyr Ala Leu Glu Lys Ile Leu Pro Phe Met 
            420                 425                 430         


Lys Glu Gly Tyr Gln Tyr Asn Glu Ala Cys Glu Lys Ala Gly Tyr Asn 
        435                 440                 445             


Phe Lys Ala Ile Tyr Glu Gly Val Arg Thr Lys Lys Leu Pro Val Ile 
    450                 455                 460                 


Glu Ile Asp Glu Ile Val Asn Pro Val Val Asn Arg Ala Leu Ala Gln 
465                 470                 475                 480 


Thr Arg Lys Val Ile Asn Ser Val Ile Asp Lys Tyr Gly Ser Pro Val 
                485                 490                 495     


Arg Ile Asn Ile Glu Leu Ala Arg Asp Leu Ala Lys Asn Phe Lys Asp 
            500                 505                 510         


Arg Lys Leu Ile Glu Lys Glu Gln Lys Glu Asn Arg Ala Asn Leu Asp 
        515                 520                 525             


Lys Ile Arg Glu Asn Leu Arg Glu Leu Met Asp Lys Glu Pro Thr Ala 
    530                 535                 540                 


Val Glu Val Leu Lys Tyr Arg Leu Trp Glu Gln Gln Arg Gly Glu Cys 
545                 550                 555                 560 


Ala Tyr Thr Gln Ser Gln Ile Ala Leu Glu Arg Leu Phe Ser Pro Gly 
                565                 570                 575     


Tyr Cys Glu Ile Asp His Ile Ile Pro Phe Ser Arg Ser Phe Asp Asp 
            580                 585                 590         


Ser Leu Ser Asn Lys Ile Leu Val Leu Gly Ser Glu Asn Gln Arg Lys 
        595                 600                 605             


Gly Asn Lys Ile Pro Tyr Glu Tyr Phe Gly Glu Asp Ser Glu Arg Trp 
    610                 615                 620                 


Asn Ser Phe Glu Ile Trp Val Lys Gly Ser Tyr Leu Asn Tyr Lys Lys 
625                 630                 635                 640 


Lys Ala Asn Leu Leu Lys Lys Lys Phe Thr Glu Glu Glu Gln Arg Asp 
                645                 650                 655     


Trp Lys Ala Arg Asn Leu Gln Asp Thr Lys Tyr Ile Cys Arg Tyr Ile 
            660                 665                 670         


Ser Asn Phe Val Asn Asn Arg Leu Glu Phe Lys Glu Ser Glu Asn Lys 
        675                 680                 685             


Gln Lys Val Ile Thr Val Asn Gly Arg Ala Thr Ala Tyr Leu Arg Ser 
    690                 695                 700                 


Arg Trp Gly Leu Asn Lys Val Arg Glu Asp Gly Asp Lys His His Ala 
705                 710                 715                 720 


Leu Asp Ala Ala Val Val Gly Val Thr Thr Gln Gly Met Val Gln Lys 
                725                 730                 735     


Ile Ser Lys Tyr Ser Lys Ala His Glu Leu Tyr Thr Val Arg Lys Asn 
            740                 745                 750         


Asp Glu Phe Val Asp Val Glu Thr Gly Glu Cys Val Asp Val Asp Glu 
        755                 760                 765             


Tyr Lys Glu Ile Arg Lys Asp Ile Leu Pro Arg Pro Trp Arg Gly Phe 
    770                 775                 780                 


Ser Glu Glu Leu Lys Leu Arg Leu Ser Asp Asn Pro Leu Glu Glu Leu 
785                 790                 795                 800 


Lys Lys Leu Pro Ile Glu Thr Tyr Asn Glu Glu Phe Ile Lys Glu Asn 
                805                 810                 815     


Val Lys Pro Ile Phe Val Ser Arg Val Pro Phe Arg Lys Ile Ser Gly 
            820                 825                 830         


Lys Leu Phe Lys Glu Thr Ile Tyr Ser Glu Arg Ala Phe Lys Asp Gly 
        835                 840                 845             


Tyr Phe Val Ser Lys Lys Ser Leu Val Asp Leu Lys Arg Gly Asp Leu 
    850                 855                 860                 


Asp Asn Phe Tyr Asn Tyr Glu Cys Asp Lys Lys Leu Tyr Asp Ala Ile 
865                 870                 875                 880 


Ser Glu Arg Met Lys Glu Phe Asn Tyr Asn Gly Lys Lys Ala Phe Glu 
                885                 890                 895     


Ser Pro Phe Arg Lys Pro Thr Lys Ser Gly Lys Glu Gly Pro Val Val 
            900                 905                 910         


Arg Ser Val Lys Ile Lys Ser Ala Ala Pro Phe Lys Asp Gly Ile Pro 
        915                 920                 925             


Met Asn Glu Gly Leu Val Ala Lys Glu Gly Met Val Arg Ile Asp Ile 
    930                 935                 940                 


Tyr Glu Lys Glu Gly Lys Tyr Phe Val Val Pro Val Tyr Arg Tyr Gln 
945                 950                 955                 960 


Leu Ala Lys Gly Ile Val Pro Lys Arg Ala Ala Ile Ala Gly Lys Ser 
                965                 970                 975     


Glu Leu Lys Trp Pro Ile Met Asp Gln Thr Tyr Asn Phe Lys Phe Ser 
            980                 985                 990         


Ile Phe Lys Asn Asp Leu Ile Glu  Ile Lys Tyr Ala Lys  Lys Asp Gly 
        995                 1000                 1005             


Tyr Phe  Gly Tyr Tyr Asp Gly  Phe Asp Arg His Thr  Ala Ser Leu 
    1010                 1015                 1020             


Val Ile  Glu Asn His Asp Asn  Ser Asp Arg Tyr Arg  Gly Ile Gly 
    1025                 1030                 1035             


Val Lys  Thr Gly Val Val Glu  Phe Asn Lys Tyr Glu  Val Asp Val 
    1040                 1045                 1050             


Leu Gly  Asn Tyr His Lys Val  Asn Leu Gly Gly Asn  
    1055                 1060                 1065 


<210>  560
<211>  1066
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  560

Met Ser Glu Arg Val Val Arg Arg Ile Leu Gly Ile Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Cys Gly Trp Gly Val Val Asp Ile Ser Gly Ala Gly Gly Gly 
            20                  25                  30          


Ile Ile Ala Thr Gly Val Arg Cys Phe Asp Ala Pro Leu Ile Asp Lys 
        35                  40                  45              


Thr Gly Glu Pro Lys Ser Ala Thr Arg Arg Thr Ala Arg Gly Gln Arg 
    50                  55                  60                  


Arg Ile Val Arg Arg Arg Arg Gln Arg Met Asn Gly Val Arg Arg Leu 
65                  70                  75                  80  


Leu Cys Glu Phe Gly Leu Leu Pro Asp Pro Arg Pro Asp Ala Leu Asn 
                85                  90                  95      


Gln Ala Met Arg Arg Ile Ser Thr Ala Ser Ala Ala Ala Gln Val Thr 
            100                 105                 110         


Pro Trp Thr Leu Arg Ala Ala Ala His Gln Arg Leu Leu Ser Asn Glu 
        115                 120                 125             


Glu Leu Ala Val Val Leu Gly His Ile Ala Arg His Arg Gly Phe Arg 
    130                 135                 140                 


Ser Asn Ala Lys Asn Glu Ala Gly Ala Asn Ala Ala Asp Glu Thr Ser 
145                 150                 155                 160 


Lys Met Lys Lys Ala Met Glu Ala Thr Arg Glu Gly Leu Ala Lys Tyr 
                165                 170                 175     


His Ala Phe Gly Asp Met Ile Ala Asn Asp Pro Lys Phe Ala Asn Arg 
            180                 185                 190         


Lys Arg Asn Arg Asp Lys Asp Tyr Ser His Thr Ala Lys Arg Ser Asp 
        195                 200                 205             


Leu Glu Asp Glu Val Arg Ala Ile Leu Arg Ala Gln Leu Arg Phe Gly 
    210                 215                 220                 


Ser Ala Ala Ala Thr Glu Thr Leu Ala Gln Thr Phe Ala Asp Val Ala 
225                 230                 235                 240 


Phe Phe Gln Arg Pro Leu Gln Asp Ser Glu Asp Arg Val Gly Asp Cys 
                245                 250                 255     


Pro Phe Glu Pro Gly Gln Lys Arg Ala Ala Arg Arg Ala Pro Ser Phe 
            260                 265                 270         


Glu Leu Phe Arg Phe Leu Ser Arg Leu Ala Asn Leu Lys Leu Ala Val 
        275                 280                 285             


Gly Arg Ser Pro Glu Arg Arg Leu Thr Ala Glu Glu Ile Ala Leu Ala 
    290                 295                 300                 


Ala Lys Gly Phe Gly Glu Thr Lys Lys Thr Ile Thr Phe Lys Ser Leu 
305                 310                 315                 320 


Arg Glu Ala Leu Asp Leu Asp Pro Asn Ala Arg Phe Ser Gly Ile Gly 
                325                 330                 335     


Lys Asp Lys Glu Ala Ser Leu Asp Val Val Ala Arg Thr Gly Gly Ala 
            340                 345                 350         


Ala Tyr Gly Thr Lys Thr Leu Lys Asp Ala Leu Gly Asp Ala Pro Trp 
        355                 360                 365             


Arg Ser Leu Ser Arg Thr Pro Glu Thr Leu Asp Arg Ile Ala Glu Ile 
    370                 375                 380                 


Leu Ser Phe Arg Glu Asp Ile Thr Ser Ile Arg Asn Gly Leu Glu Asp 
385                 390                 395                 400 


Leu Gly Leu Asp Ser Leu Val Val Glu Ala Leu Met Gln Ala Ala Ala 
                405                 410                 415     


Asn Gly Asp Phe Lys Glu Phe Thr Arg Ala Gly His Ile Ser Ala Arg 
            420                 425                 430         


Ala Ala Arg Asn Ile Ile Pro Gly Leu Arg Glu Gly Leu Val Tyr Ser 
        435                 440                 445             


Glu Ala Cys Ala Arg Val Gly Tyr Asp His Ala Ala Arg Leu Ser Val 
    450                 455                 460                 


Pro Leu Asp Gln Val Gly Ser Pro Val Thr Arg Lys Ala Leu Ser Glu 
465                 470                 475                 480 


Ala Leu Lys Gln Val Arg Ala Val Ala Arg Glu Tyr Gly Pro Ile Asp 
                485                 490                 495     


Tyr Phe His Ile Glu Leu Ala Arg Ser Val Gly Lys Ser Ala Glu Glu 
            500                 505                 510         


Arg Lys Gln Leu Thr Asp Gly Ile Glu Ala Arg Asn Val Glu Lys Ala 
        515                 520                 525             


Lys Arg Arg Lys Gln Ala Glu Glu His Leu Gly Arg Ala Pro Ser Asp 
    530                 535                 540                 


Asp Glu Leu Leu Arg Tyr Glu Leu Ala Lys Glu Gln Asn Phe Lys Cys 
545                 550                 555                 560 


Ile Tyr Ser Gly Asp Ala Ile Asp Pro Ala Gly Val Ala Ala Asn Asp 
                565                 570                 575     


Thr Arg Tyr Gln Val Asp His Ile Leu Pro Trp Ser Arg Phe Gly Asp 
            580                 585                 590         


Asp Ser Tyr Leu Asn Lys Thr Leu Cys Thr Ala Arg Ser Asn Gln Asn 
        595                 600                 605             


Lys Arg Gly Arg Thr Pro Phe Glu Trp Phe Glu Ala Asp Lys Thr Asp 
    610                 615                 620                 


Val Glu Trp Met Glu Tyr Val Ala Arg Val Glu Asn Leu Gln Glu Val 
625                 630                 635                 640 


Lys Gly Arg Lys Lys Arg Asn Tyr Ser Ile Lys Asp Ala Ala Ala Ile 
                645                 650                 655     


Glu Asp Lys Phe Lys Ala Arg Asn Leu Thr Asp Thr Gln Trp Ala Thr 
            660                 665                 670         


Arg Leu Leu Ala Asp Glu Leu Ser Arg Met Phe Pro Pro Arg Glu Cys 
        675                 680                 685             


Glu Arg Ala Ile Cys Gly Arg Ala Asp Gly Gly Asn Asp Gly Leu Thr 
    690                 695                 700                 


Ile Val Glu Glu Arg Arg Val Phe Thr Arg Pro Gly Ala Ile Thr Ser 
705                 710                 715                 720 


Lys Leu Arg Arg Ala Trp Gly Leu Glu Gly Leu Lys Lys Gln Asp Gly 
                725                 730                 735     


Lys Arg Val Glu Asp Asp Arg His His Ala Val Asp Ala Leu Val Leu 
            740                 745                 750         


Ala Ala Thr Thr Glu Ser Leu Leu Gln Arg Leu Thr Val Glu Val Gln 
        755                 760                 765             


Arg Arg Glu Arg Glu Gly Arg Pro Asp Asp Ile Phe His Cys Ala Glu 
    770                 775                 780                 


Pro Trp Arg Gly Phe Arg Ala Asp Val Arg Arg Thr Val Tyr Gly Ser 
785                 790                 795                 800 


Glu Thr Met Pro Gly Ile Phe Val Ser Arg Ala Glu Arg Arg Arg Ala 
                805                 810                 815     


Arg Gly Lys Ala His Asp Ala Thr Ile Lys Gln Ile Arg Glu Ile Glu 
            820                 825                 830         


Gly Glu Arg Leu Val Phe Glu Arg Lys Pro Val Glu Lys Leu Thr Asp 
        835                 840                 845             


Lys Asp Leu Glu Lys Ile Pro Ile Pro Lys Pro Tyr Gly Gln Val Ser 
    850                 855                 860                 


Asp Pro Lys Arg Leu Arg Asp Glu Leu Val Glu Ser Leu Arg Ala Trp 
865                 870                 875                 880 


Ile Ala Ala Gly Lys Pro Lys Asp Arg Pro Pro Leu Ser Pro Lys Gly 
                885                 890                 895     


Asp Val Ile Arg Lys Val Arg Ile Gln Thr Asn Asp Lys Val Ser Val 
            900                 905                 910         


Glu Ile Asn Gly Gly Thr Val Asp Arg Gly Asp Met Ala Arg Val Asp 
        915                 920                 925             


Val Phe Arg Lys Lys Asn Lys Lys Ala Val Trp Glu Tyr Tyr Val Val 
    930                 935                 940                 


Pro Ile Tyr Pro His Gln Ile Val Ala Leu Asn Asp Pro Pro Asp Arg 
945                 950                 955                 960 


Ala Val Ile Ala Tyr Ala Glu Asp Lys Asp Trp Lys Glu Ile Asp Ser 
                965                 970                 975     


Ser Tyr Glu Phe Leu Trp Ser Leu Phe Gly Leu Ser Tyr Val Glu Ile 
            980                 985                 990         


Ser Lys Ala Asn Gly Glu Cys Ile  Asp Gly Tyr Phe Arg  Gly Leu His 
        995                 1000                 1005             


Arg Gly  Thr Gly Ala Ala Ser  Val Cys Lys His Ile  Ser Leu Gly 
    1010                 1015                 1020             


Lys Asp  Ala Thr Val Ser Gly  Ile Gly Leu Lys Thr  Leu Ala Ser 
    1025                 1030                 1035             


Phe Lys  Lys Phe Thr Ile Asp  Arg Leu Gly Arg Lys  Phe Glu Ile 
    1040                 1045                 1050             


Pro Arg  Glu Val Arg Thr Trp  Arg Gly Glu Ala Cys  Thr 
    1055                 1060                 1065     


<210>  561
<211>  1066
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  561

Met Ser His Leu Val Leu Gly Leu Asp Leu Gly Pro Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Val Asn Asp Asp Pro Glu His Pro Val Glu Ser Lys Leu 
            20                  25                  30          


Val Asp Leu Gly Val Arg Val Phe Pro Glu Gly Val Asp Asn Phe Asp 
        35                  40                  45              


Thr Ser Lys Glu Leu Ser Arg Asn Glu Asp Arg Arg Val Ala Arg Gly 
    50                  55                  60                  


Met Arg Arg Gln Ile Arg Arg Arg Ala Arg Arg Arg Gln Gln Leu Lys 
65                  70                  75                  80  


Gln Ala Leu Ile Asp Ser Lys Leu Trp Pro Ser Asp Pro Asn Asp Glu 
                85                  90                  95      


Val Val Leu Tyr Asn Thr Asn Pro Tyr Glu Leu Arg Ala Arg Ala Leu 
            100                 105                 110         


Lys Glu Arg Leu Glu Pro His Glu Val Gly Arg Val Leu Leu His Leu 
        115                 120                 125             


Asn Gln Arg Arg Gly Phe Leu Ser Asn Arg Lys Lys Asp Arg Gly Asp 
    130                 135                 140                 


Lys Glu Val Gln Gly Met Leu Ala Glu Ile Asn Glu Asn Glu Gln Gln 
145                 150                 155                 160 


Cys Lys Asp Gly Gly Phe Asp Thr Ile Gly Ala Trp Leu Ala Asp Lys 
                165                 170                 175     


Gln Ala Asn Ile Asp His Ala Asn Arg Glu Glu Asn Asp His Val Arg 
            180                 185                 190         


Asn Arg His Leu Ala Arg Ser Gln Tyr Val Asp Glu Phe Glu Ala Ile 
        195                 200                 205             


Trp Ser Val Gln Ala Lys His His Pro Lys Leu Leu Thr Glu Glu Leu 
    210                 215                 220                 


Lys Val Gly Lys Leu Gly Lys Gln Thr Tyr Pro Val Lys Pro Ile Pro 
225                 230                 235                 240 


Arg Trp His Glu His Arg Gln Gly Leu Ser Pro Leu Glu Ser Phe Gly 
                245                 250                 255     


Ile Tyr Gly Leu Ile Phe Phe Gln Arg Pro Met Tyr Trp Pro Lys Ser 
            260                 265                 270         


Val Val Gly Leu Cys Glu Leu Glu Pro Lys Glu Arg Arg Cys Gln Arg 
        275                 280                 285             


Ser Asp Arg Arg Gln Gln Arg Phe Arg Leu Leu Gln Glu Val Asn Asn 
    290                 295                 300                 


Leu Lys Tyr Val Asp Pro Asp Thr His Leu Glu Gln Lys Leu Asp Asp 
305                 310                 315                 320 


Lys Gln Arg Arg Leu Leu Leu Asp Lys Leu Ser His Thr Lys Glu Met 
                325                 330                 335     


Thr Phe Asp Gln Ile Arg Lys Ala Leu Asp Phe Leu Glu Ser Val Lys 
            340                 345                 350         


Phe Asn Leu Glu Arg Gly Glu Arg Ser Arg Leu Lys Gly Val Pro Ile 
        355                 360                 365             


Asp Ala Leu Phe Ala Ala Lys Asp Val Leu Gly Gln Glu Trp Tyr Asp 
    370                 375                 380                 


Arg Pro Glu Gln Gln Lys Thr Glu Ile Val Ala Val Leu Leu Asp Asn 
385                 390                 395                 400 


Glu Arg Asp Asp Asp Arg Ile Ile Ala Arg Ala Val Ala Glu Trp Gly 
                405                 410                 415     


Met Thr Ala Lys Glu Ala Glu Ala Met Leu Gly Val Asp Leu Pro Ala 
            420                 425                 430         


Gly Tyr Ala Asn Leu Ser Cys Lys Ala Ile Glu Lys Leu Leu Pro His 
        435                 440                 445             


Met Glu Arg Gly Leu Leu Tyr Met Asp Asp Asp Glu Ser Asn Ser Ala 
    450                 455                 460                 


Met His Ala Ala Gly Tyr Leu Arg Arg Asp Gln Leu Arg Arg Arg Ile 
465                 470                 475                 480 


Phe Asp Val Leu Pro Asp Pro Ala Arg Thr Pro Asn Cys Pro Ile Gly 
                485                 490                 495     


Asp Ile Pro Asn Pro Val Val Lys Arg Thr Leu Thr Glu Ile Arg Lys 
            500                 505                 510         


Val Val Asn Ala Ile Val Arg Glu Tyr Gly Lys Pro Asp Ala Ile His 
        515                 520                 525             


Leu Glu Leu Ala Arg Ser Val Gln Met Gly Lys Glu Lys Arg Ser Glu 
    530                 535                 540                 


Tyr Ser Lys Met Ile Arg Asp Arg Glu Ala Glu Arg Asp Gly Ile Val 
545                 550                 555                 560 


Glu Lys Leu Arg Glu Ser Gly Val Arg Val Thr Arg Asp Asn Ile Leu 
                565                 570                 575     


Lys Tyr Gln Leu Trp Glu Gln Gln Asn Gln Glu Cys Ile Tyr Ser Gly 
            580                 585                 590         


Lys Pro Ile Ser Phe Glu Lys Leu Phe Gly Glu Gly Gly Gly Val Glu 
        595                 600                 605             


Val Asp His Ile Leu Pro Arg Ser Arg Ser Leu Asp Asp Ser Gln Ala 
    610                 615                 620                 


Asn Lys Ile Val Cys Leu Arg Thr Ala Asn Ala Gly Lys Gly Asp Gln 
625                 630                 635                 640 


Thr Pro Tyr Glu Trp Leu Ala Asp Thr Gln Pro Lys Gln Tyr Glu Gln 
                645                 650                 655     


Val Cys Gln Arg Ala Gly Met Leu Met Arg Ser Gly Arg Met Pro Tyr 
            660                 665                 670         


Ala Lys Tyr Arg Arg Phe Ile Gln Arg Glu Leu Glu Leu Asp Lys Phe 
        675                 680                 685             


Ile Ala Arg Gln Leu Ala Asp Thr Gly Tyr Ile Thr Lys Ala Thr Met 
    690                 695                 700                 


Glu Tyr Leu Arg Tyr Leu Phe Asp Lys Asp His Asp Val Leu Gly Leu 
705                 710                 715                 720 


Lys Gly Gln Leu Thr Ala Glu Leu Arg Tyr His Trp Gly Leu Glu Thr 
                725                 730                 735     


Ile Leu Glu Glu Leu Pro Asp Ser Pro Ala Trp His Glu Ala Asp Ser 
            740                 745                 750         


Gly Lys Leu Arg Pro Gly Glu Lys Asn Arg Ala Asp His Arg His His 
        755                 760                 765             


Ala Ile Asp Ala Val Val Ile Ala Leu Thr Asn Arg Arg Arg Leu Val 
    770                 775                 780                 


Gln Leu Ser Asp Ile Val Lys Arg Gly Gly Ala Arg Ala His Gly Glu 
785                 790                 795                 800 


Ile Leu Glu Asp Pro Trp Pro Asn Phe Arg Glu Asn Val Val Gln Ala 
                805                 810                 815     


Ile Lys Lys Thr Asn Val Ser His Arg Val Glu Arg Lys Val Ala Gly 
            820                 825                 830         


Lys Leu His Glu Glu Thr Leu Tyr Gly Pro Thr Arg Thr Pro Gly Glu 
        835                 840                 845             


Trp Val Leu Arg Lys Pro Val Ile Ala Leu Ser Ala Asn Glu Val Glu 
    850                 855                 860                 


Arg Ile Arg Asp Ala Thr Ile Arg Gln Ile Val Val Ser Ala Leu Ala 
865                 870                 875                 880 


Asp Ala Gly Ile Asp Val Gly Arg Gly Lys Lys Pro Asp Ala Lys Lys 
                885                 890                 895     


Met Lys Glu Ala Leu Ser Lys Leu Lys Met Pro Ser Gly Val Pro Ile 
            900                 905                 910         


Arg Lys Val Arg Leu Thr Lys Pro Glu Gln Thr Ile Gln Pro Val Arg 
        915                 920                 925             


Ala Gly Arg Pro Asp Gln Ala Phe Val Lys Pro Gly Ser Thr His His 
    930                 935                 940                 


Leu Cys Ile Phe Glu Phe Thr Glu Asn Gly Lys Thr Lys Arg Asp Ala 
945                 950                 955                 960 


Val Phe Val Thr Met Leu Glu Ala Met Asn Arg Leu Lys Arg Arg Glu 
                965                 970                 975     


Pro Val Ile Gln Arg Thr His Pro Glu Arg Pro Asp Ser Arg Phe Val 
            980                 985                 990         


Met Ser Leu Ser Ala Arg Glu Met  Leu Leu Ala Asn Trp  Lys Asp Gly 
        995                 1000                 1005             


Glu Arg  Leu Leu Thr Phe Lys  Thr Ala Ser Ser Thr  Gln Gly Gln 
    1010                 1015                 1020             


Ile Trp  Phe Ala Glu His Thr  Asp Ala Arg Lys Ser  Ala Asp Tyr 
    1025                 1030                 1035             


Lys Gln  Phe Val Ala Asn Ala  Asn Thr Leu Asp Ala  Lys Lys Val 
    1040                 1045                 1050             


Thr Val  Asp Pro Leu Gly Arg  Ile Arg Trp Ala Asn  Asp 
    1055                 1060                 1065     


<210>  562
<211>  1067
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  562

Met Gly Thr Arg Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Cys Leu Phe Asn Leu Asp Gly Ser Gly Arg Pro Ile Gly Ile Arg Asp 
            20                  25                  30          


Ile Gly Val Arg Ile Phe Ser Asp Gly Arg Asp Pro Arg Ser Gly Ala 
        35                  40                  45              


Ser Leu Ala Val Glu Arg Arg Val Ala Arg Ala Met Arg Arg Arg Arg 
    50                  55                  60                  


Asp Arg Tyr Ile Arg Arg Arg Ser Ala Leu Leu Glu Ala Leu Ile Asn 
65                  70                  75                  80  


Thr Gly Leu Met Pro Ala Asp Val Gln Glu Ser Lys Ala Leu Thr Gly 
                85                  90                  95      


Cys Asp Pro Tyr Val Leu Arg Ala Gln Ala Leu Asp Arg Ala Leu Asp 
            100                 105                 110         


Ala His Leu Leu Gly Arg Val Leu Phe His Leu Asn Gln Arg Arg Gly 
        115                 120                 125             


Phe Lys Ser Asn Arg Lys Ala Glu Arg Lys Ala Gly Asp Asp Lys Glu 
    130                 135                 140                 


Gly Gly Lys Ile Ala Asp Gly Ala Lys Ala Leu Asp Leu Ala Met His 
145                 150                 155                 160 


Glu Ala Gly Ala Arg Thr Tyr Gly Glu Phe Leu Ala Gly Arg Asp Ser 
                165                 170                 175     


Arg Arg Val Arg Leu Arg Ala Glu Gly Asp Gly Tyr Asp Phe Tyr Pro 
            180                 185                 190         


Glu Arg Arg His Leu Glu Ala Glu Phe Asp Ala Ile Trp Asp Ala Gln 
        195                 200                 205             


Ala Ala Tyr Asn Pro Ala Leu Leu Thr Asp Val Val Arg Asp Arg Leu 
    210                 215                 220                 


Arg Arg Ile Ile Phe Phe Gln Arg Pro Leu Lys Ala Pro Lys Val Gly 
225                 230                 235                 240 


Gly Cys Thr Phe Phe Asn Glu Glu Pro Arg Leu Pro Lys Ala His Pro 
                245                 250                 255     


Leu Phe Gln Glu Arg Arg Leu Tyr Glu Glu Val Asn Gln Leu Glu Ile 
            260                 265                 270         


Thr Arg Ala Gly Ala Pro Ser Arg Lys Leu Thr Leu Asp Gln Arg Asp 
        275                 280                 285             


Lys Ile Ile Arg Lys Leu Lys Ser Ala Lys Thr Ala Ser Phe Ser Gly 
    290                 295                 300                 


Leu Ala Arg Leu Leu Lys Leu Ala Asp Gly Glu Ser Phe Ser Arg Val 
305                 310                 315                 320 


Thr Glu Asn Arg Thr Gly Leu Ala Gly Asp Asp Ile Phe Thr Ser Phe 
                325                 330                 335     


Ser Asp Lys Arg Cys Phe Gly Asp Arg Trp Ala His Phe Asp Pro Gly 
            340                 345                 350         


Val Gln Trp Ser Ile Ile Glu Thr Val Thr Glu Glu Glu Asp Pro Val 
        355                 360                 365             


Arg Leu Arg Asp Trp Leu Met Ala Thr His Gly Val Thr Val Glu Gln 
    370                 375                 380                 


Ala Glu Ala Ile Gly Arg Val Arg Leu Pro Glu Gly His Gly Arg Leu 
385                 390                 395                 400 


Gly Glu Thr Ala Ser Arg Leu Ile Leu Ala Gln Leu Lys Ser Gly Ser 
                405                 410                 415     


Lys Asp Gly His Pro Leu Thr Tyr Asn Glu Ala Val Glu Ala Ala Leu 
            420                 425                 430         


Gly Lys Ser His Ser Asp Phe Arg Thr Gly Glu Ile Leu Glu Gln Leu 
        435                 440                 445             


Pro Tyr Tyr Gly Glu Ile Leu Ser Arg Glu Ile Pro Pro Gly Ser Leu 
    450                 455                 460                 


Asp Pro Asp Asp Asp Asp Glu Ile Arg Trp Gly Lys Ile Thr Asn Pro 
465                 470                 475                 480 


Thr Val His Ile Gly Leu Asn Gln Leu Arg Arg Leu Leu Asn Ala Leu 
                485                 490                 495     


Ile Lys Ala His Gly Arg Pro Asp Glu Ile Val Val Glu Leu Ala Arg 
            500                 505                 510         


Glu Leu Lys Leu Ser Glu Lys Asp Lys Ala Glu His Asn Arg Arg Ile 
        515                 520                 525             


Gly Ala Asn Thr Arg Ala Ala Glu Ala Arg Ser Lys Lys Leu Leu Glu 
    530                 535                 540                 


Ser Gly Gln Pro Asp Thr Gly Ala Asn Arg Ser Leu Leu Lys Leu Trp 
545                 550                 555                 560 


Glu Asp Leu Asn Pro Asn Asn Pro Leu Asp Arg Cys Cys Pro Phe Cys 
                565                 570                 575     


Gly Arg His Ile Gly Gln Met Ala Leu Phe Ser Gly Glu Thr Glu Ile 
            580                 585                 590         


Glu His Ile Ile Pro Tyr Ser Arg Ser Leu Asp Asp Ser Ala Ala Asn 
        595                 600                 605             


Lys Val Ile Ala His Arg Ser Cys Asn Arg Glu Lys Gly Asn Arg Thr 
    610                 615                 620                 


Pro Trp Glu Ala Trp Gly His Thr Glu Arg Trp Ala Ile Ile Ala Glu 
625                 630                 635                 640 


Gln Val Ser Arg Leu His Lys Ser Lys Gln Trp Arg Phe Ala Pro Asp 
                645                 650                 655     


Ala Ile Glu Arg Phe Asn Gln Glu Gly Gly Phe Ile Ala Arg Gln Leu 
            660                 665                 670         


Ser Asp Thr Gln Tyr Leu Ser Arg Met Ala Arg Thr Tyr Leu Ser Ser 
        675                 680                 685             


Leu Tyr Pro Asp Thr Ala Gly Val Tyr Val Ile Pro Gly Arg Met Thr 
    690                 695                 700                 


Ala Met Leu Arg Arg Leu Trp Gly Leu Asn Glu Leu Leu Pro Asp His 
705                 710                 715                 720 


Asn Tyr Val Glu Asn Pro His Ser Gly Ala Pro Lys Asn Arg Leu Asp 
                725                 730                 735     


His Arg His His Ala Ile Asp Ala Ala Val Val Gly Val Thr Thr Arg 
            740                 745                 750         


Gly Leu Leu Asn Glu Ile Ser Arg Ala Ala Gly Arg Ala Glu Arg Gln 
        755                 760                 765             


Asp Leu Asp Arg Leu Phe Ile Asp Leu Pro Lys Pro Trp Glu Thr Phe 
    770                 775                 780                 


Arg Glu Asp Leu His Glu Ser Leu Ala Arg Val Val Gly Ser His Lys 
785                 790                 795                 800 


Ala Asp His Gly Arg Lys Gly Val Pro Ala Lys Gly Lys Asp Ala Thr 
                805                 810                 815     


Ala Ala Arg Leu His Asn Glu Thr Ala Tyr Gly Leu Thr Gly Leu Thr 
            820                 825                 830         


Ser Glu Ser Gly Leu Pro Ile Val Val His Arg Val Pro Leu Leu Ser 
        835                 840                 845             


Leu Lys Pro Asp Asp Leu Ser Asn Pro Ile Arg Ile Pro Asp Ala Ala 
    850                 855                 860                 


Leu Gln Arg Ala Leu Arg Glu Ala Thr Asp Gly Lys Ser Gly Lys Asp 
865                 870                 875                 880 


Phe Gln Glu Ala Leu Ala Gln Phe Ser Lys Ser Asp Pro Val Phe Lys 
                885                 890                 895     


Gly Ile Arg His Val Arg Val Arg Glu Thr Leu Lys Val Ile Pro Ile 
            900                 905                 910         


Arg Asp Ala Ser Gly His Ala Phe Lys Ala Tyr Lys Gly Asp Ala Asn 
        915                 920                 925             


Ala Arg Tyr Asp Val Trp Arg Met Pro Asp Gly Lys Trp Val Ser Ser 
    930                 935                 940                 


Trp Arg Asp Arg Asp Gly Lys Ala His Ser Gly Ile Ile Thr Met Phe 
945                 950                 955                 960 


Ala Ala His Gln Ala Gly Gln Pro Val Gln Arg Pro His Pro Ala Ala 
                965                 970                 975     


Lys Arg Val Leu Ser Leu Arg Gln Asn Asp Leu Val Ala Val Glu Pro 
            980                 985                 990         


Gln Gly Gln Pro Arg Lys Ile Met  Arg Val Val Lys Phe  Ser Ser Gly 
        995                 1000                 1005             


Gly Gln  Ile Thr Phe Ala Glu  His Gln Glu Ala Gly  Pro Leu Lys 
    1010                 1015                 1020             


Ala Arg  Asp Ala Thr Ser Pro  Asp Ile Asp Pro Phe  Lys Tyr Phe 
    1025                 1030                 1035             


Tyr Ser  Ser Ala Gly Gly Leu  Gln Lys Ala Arg Ala  Arg Gln Ile 
    1040                 1045                 1050             


Arg Ile  Asp Glu Leu Gly Arg  Ile Phe Asp Pro Gly  Pro Arg 
    1055                 1060                 1065         


<210>  563
<211>  1068
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  563

Met Lys Tyr Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Cys Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Gln Glu Lys His Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Lys Ala Glu Asn Pro Lys Asp Gly Lys Ser Leu Ala Thr 
        35                  40                  45              


Pro Arg Arg Asp Ala Arg Ser Thr Arg Arg Thr Leu Arg Arg Lys Lys 
    50                  55                  60                  


His Arg Met Gln Arg Ile Lys Ile Leu Leu Val Lys His Gly Leu Leu 
65                  70                  75                  80  


Ser Lys Thr Glu Ile Asp His Leu Tyr Glu Ser Ala Thr Glu Ile Asp 
                85                  90                  95      


Val Trp Tyr Leu Arg Leu Asn Ala Leu Glu Arg Arg Leu Asn Pro Lys 
            100                 105                 110         


Glu Phe Ala Arg Val Leu Ile His Leu Ser Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Glu Thr Thr Leu Ser Glu Asn Gly Gln Ile Leu Glu 
    130                 135                 140                 


Asn Ile Ser Glu Asn Leu Gln Ile Met Glu Gln Lys Asn Tyr Arg Thr 
145                 150                 155                 160 


Val Gly Glu Met Ile Leu Lys Asp Lys Arg Phe Glu Asn His Lys Arg 
                165                 170                 175     


Asn Lys Asp Gly Thr Tyr Ile Gly Thr Val Thr Arg Gln Gln Leu Gln 
            180                 185                 190         


Glu Glu Ile Gln Met Ile Phe Asn Ala Gln Arg Leu Tyr Lys Asn Asn 
        195                 200                 205             


His Ala Thr Glu Lys Phe Glu Ser Ser Tyr Leu Glu Ile Trp Ala Ser 
    210                 215                 220                 


Gln Arg Pro Tyr Ala Ser Lys Asp Gln Ile Glu Lys Met Ile Gly Tyr 
225                 230                 235                 240 


Cys Thr Leu Glu Pro Lys Glu Lys Arg Val Pro Lys Ala Ser Trp Ser 
                245                 250                 255     


Phe Gln Tyr Phe Val Ala Leu Gln Thr Ile Asn Asn Leu Arg Leu Ile 
            260                 265                 270         


Ser Lys Asp Arg Ile Glu Glu Leu Ser Phe Glu Glu Lys Lys Arg Ile 
        275                 280                 285             


Met Asn Leu Ala Leu Glu Lys Ser Ile Val Lys Tyr Ile Asp Ile Arg 
    290                 295                 300                 


Lys Leu Leu Ser Val Pro Lys Glu Phe Gln Phe Asn Ser Leu Ser Tyr 
305                 310                 315                 320 


Ser Ala Asp Ile Val Asp Thr Thr Val Glu Asn Lys Lys Cys Ile Glu 
                325                 330                 335     


Leu Lys Glu Tyr His Ser Ile Asn Lys Val Tyr Lys Gln Ile Tyr Gly 
            340                 345                 350         


Gln Asn Ala Leu Asn Leu Leu Pro Lys Asp Tyr Asp Thr Ile Ala Tyr 
        355                 360                 365             


Gly Leu Thr Ile Phe Lys Asp Asp Lys Asp Ile Gln Glu Tyr Leu Gln 
    370                 375                 380                 


Asn Lys Tyr Val Asn Ala Lys Gly Lys Pro Ile Ser Asn Leu Ala Asn 
385                 390                 395                 400 


Lys Thr Tyr Asp Asp Thr Phe Ile Gln Val Leu Leu Thr Leu Asn Phe 
                405                 410                 415     


Ser Lys Met Gly His Leu Ser Phe Lys Ala Leu Lys Asn Ile Ile Pro 
            420                 425                 430         


Phe Leu Glu Glu Gly Leu Ser Tyr Asp Lys Ala Cys Glu Lys Ala Gly 
        435                 440                 445             


Tyr Asn Phe Lys Gly Thr Ser Tyr Thr Lys Lys Thr Lys Tyr Leu Pro 
    450                 455                 460                 


Val Ile Pro Gln Asn Thr Asn Pro Val Val His Arg Ala Leu Ser Gln 
465                 470                 475                 480 


Thr Arg Lys Val Ile Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Asn 
                485                 490                 495     


Ala Ile His Ile Glu Thr Ala Arg Glu Leu Ser Lys Thr Phe Gln Glu 
            500                 505                 510         


Arg Lys Glu Ile Asp Ser Met Tyr Gln Asp Asn Ser Lys Lys Asn Glu 
        515                 520                 525             


His Ala Ile Cys Lys Leu Lys Glu Leu Gly Asn Ile Asn Pro Ser Gly 
    530                 535                 540                 


Ile Asn Ile Val Lys Phe Lys Leu Trp Asn Glu Gln Asp Gly Arg Cys 
545                 550                 555                 560 


Met Tyr Thr Gly Lys Tyr Ile Glu Pro His Arg Leu Phe Glu Glu Gly 
                565                 570                 575     


Tyr Thr Glu Val Asp His Ile Leu Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Asn Asn Lys Val Leu Thr Leu Gly Thr Glu Asn Gln Arg Lys 
        595                 600                 605             


Gly Asn Lys Thr Pro Tyr Glu Tyr Met Arg Asn Asn Ser Ile Trp Asp 
    610                 615                 620                 


Glu Phe Glu Thr Arg Val Gln Ser Asn Lys Lys Ile Asn Lys Lys Lys 
625                 630                 635                 640 


Gln Gln Lys Leu Leu Leu Gln Asn Phe Ser His Ala Arg Glu Gln Glu 
                645                 650                 655     


Phe Ile Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ala Thr Ile Tyr Leu 
            660                 665                 670         


Ser Ser Leu Ile Gln Gln His Leu Ile Phe Ser Glu Ser Ser Arg Glu 
        675                 680                 685             


Lys Lys Val His Thr Val Ser Gly Ile Ile Thr Ser His Leu Arg Ser 
    690                 695                 700                 


Arg Trp Gly Phe Asn Lys Asp Arg Lys Glu Gly His Ile His His Ala 
705                 710                 715                 720 


Leu Asp Ala Val Ile Val Ala Val Thr Ser Asp His Met Ile Gln Arg 
                725                 730                 735     


Val Thr Lys Tyr Tyr Lys Leu Lys Glu Leu Asn Arg Asn Arg Gln Ala 
            740                 745                 750         


Lys Arg Met Gln Phe Pro Glu Pro Trp Glu Gly Phe Arg Leu Glu Leu 
        755                 760                 765             


Glu Ala Arg Ile Ser Pro Asn Thr Gln Gln Tyr Leu Lys Gly Ile Arg 
    770                 775                 780                 


Phe Lys Asn Tyr Ala Asp Val Asn Leu Ser Glu Ile Lys Pro Ile Phe 
785                 790                 795                 800 


Val Ser Arg Met Pro Lys Arg Ser Ile Thr Gly Glu Leu His Gln Glu 
                805                 810                 815     


Thr Ile Arg Lys Phe Leu Gly Tyr Asn Glu Lys Gly Lys Val Leu Thr 
            820                 825                 830         


Ala Thr Lys Thr Lys Leu Glu Asp Ile Pro Phe Asp Ala Asn Gly Asp 
        835                 840                 845             


Phe Pro Met Phe Gly Lys Glu Thr Asp Leu Tyr Thr Tyr Asn Ala Ile 
    850                 855                 860                 


Lys Glu Arg Tyr Leu Ser His Lys Lys Asp Lys Ser Lys Ser Phe Gln 
865                 870                 875                 880 


Glu Pro Leu Tyr Lys Pro Thr Lys Ser Gly Gly Ile Gly Pro Leu Ile 
                885                 890                 895     


Lys Ser Ile Lys Ile Met Asp Thr Arg Thr Ile Val Asn Pro Val Asn 
            900                 905                 910         


Gln Gly Lys Gly Val Val Tyr Asn Ser Lys Ile Ala Arg Thr Asp Val 
        915                 920                 925             


Phe Lys Lys Gly Glu Lys Tyr Tyr Leu Ile Pro Ile Tyr Thr Ile Asp 
    930                 935                 940                 


Leu Met Lys Asn Thr Leu Pro Gln Lys Ala Ile Thr Ala Gly Lys Gly 
945                 950                 955                 960 


Tyr Glu Asp Trp Thr Thr Ile Asp Pro Ser Phe Thr Phe Leu Phe Ser 
                965                 970                 975     


Leu Phe Pro Asn Asp Leu Ile Gln Ile Val Pro Ser Lys Asn Lys Thr 
            980                 985                 990         


Ile Lys Ala Arg Thr Thr Val Ser  Lys Lys Glu Val Leu  Leu Pro Ser 
        995                 1000                 1005             


Leu Thr  Gly Tyr Phe Lys Gly  Val His Ser Gly Thr  Ala Gly Ile 
    1010                 1015                 1020             


Thr Val  Glu Thr His Asp Gly  Ser Ile Ile Ala Asn  Val Gly Ser 
    1025                 1030                 1035             


Lys Gln  Leu Leu Ser Phe Glu  Lys Tyr Gln Val Asp  Val Leu Gly 
    1040                 1045                 1050             


His Tyr  Thr Lys Ile Lys Lys  Glu Lys Arg Ile Gly  Met Ala Ile 
    1055                 1060                 1065             


<210>  564
<211>  1068
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  564

Met Asn Lys Ala Val Ala Tyr Arg Leu Ala Leu Asp Leu Gly Ser Thr 
1               5                   10                  15      


Ser Leu Gly Trp Ala Ile Phe Arg Leu Asn Glu Ala Arg Glu Pro Thr 
            20                  25                  30          


Ala Ile Ile Arg Ala Gly Val Arg Ile Phe Ser Asp Gly Arg Asn Ala 
        35                  40                  45              


Asn Ser Glu Pro Leu Ala Val His Arg Arg Val Ala Arg Ala Met Arg 
    50                  55                  60                  


Arg Arg Arg Asp Arg Leu Leu Lys Arg Lys Lys Arg Met His Asp Gln 
65                  70                  75                  80  


Leu Val Gln His Gly Phe Phe Pro Ala Glu Val Ala Glu Arg Lys Glu 
                85                  90                  95      


Leu Glu Arg Leu Asn Pro Tyr Gln Leu Arg Ala Lys Gly Leu His Glu 
            100                 105                 110         


Ala Leu Thr Pro Gly Glu Phe Ala Arg Ala Leu Phe His Ile Asn Gln 
        115                 120                 125             


Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Arg Lys Asp Asn Asp 
    130                 135                 140                 


Ser Gly Ala Leu Lys Gln Ala Ile Ser Glu Leu Arg Lys Arg Ile Gln 
145                 150                 155                 160 


Asp Ser Asp Cys Ala Thr Ala Gly Glu Trp Phe Trp Lys Glu Arg Met 
                165                 170                 175     


Gln Gln Lys Pro Glu Gly Val Arg Gly Gln Trp Val Arg Ala Arg Tyr 
            180                 185                 190         


Arg Lys Thr Pro Ser Thr Thr Asp Glu Gly Lys Lys Arg Ile Gly Tyr 
        195                 200                 205             


Asp Leu Tyr Val Asp Arg Ala Met Val Glu Gln Glu Phe Asp Ala Leu 
    210                 215                 220                 


Trp Ala Ala Gln Ala Ala Leu Gln Pro Gly Leu Phe Thr Glu Ala Ala 
225                 230                 235                 240 


Arg Ala Glu Leu Lys Asp Thr Leu Leu Tyr Gln Arg Asp Leu Arg Pro 
                245                 250                 255     


Val Lys Pro Gly Arg Cys Thr Leu Leu Pro Ala Glu Glu Arg Ala Pro 
            260                 265                 270         


Leu Ala Leu Pro Ser Thr Gln Arg Phe Arg Ile Leu Gln Glu Val Asn 
        275                 280                 285             


Asn Leu Arg Leu Leu Asp Glu Ala Leu Arg Glu Val Pro Leu Asn Leu 
    290                 295                 300                 


Ala Gln Arg Asp Ala Val Val Ser Ala Leu Glu Gly Lys Lys Glu Leu 
305                 310                 315                 320 


Ser Phe Ala Ala Ile Arg Lys Leu Leu Thr Leu Ser Gly Lys Phe Asn 
                325                 330                 335     


Leu Glu Asp Glu Lys Arg Ala Glu Leu Lys Gly Asn Ala Thr Ser Val 
            340                 345                 350         


Ile Leu Ala Arg Lys Asp Leu Phe Gly Asp Ala Trp Ala Gly Phe Asp 
        355                 360                 365             


Ala Ala Val Gln Asp Glu Ile Val Trp Arg Leu Val Ser Gln Glu Ser 
    370                 375                 380                 


Glu Gly Ala Leu Ile Ala Trp Leu Gln Gln His Thr Gly Val Asp Gly 
385                 390                 395                 400 


Val Cys Ala Glu Ala Ile Val Asn Thr Arg Leu Pro Asp Gly Tyr Gly 
                405                 410                 415     


Arg Leu Ser Arg Lys Ala Leu Glu Arg Ile Val Pro Ala Leu Gln Arg 
            420                 425                 430         


Glu Val Cys Thr Tyr Asp Lys Ala Val Gln Ala Ala Gly Phe Ala His 
        435                 440                 445             


His Ser Asp Leu Gly Phe Asp Phe Asp Tyr Ala Glu Asp Glu Val Gln 
    450                 455                 460                 


Gln Val Gly Glu His Thr Ile Ala Ser Thr Gly Glu Val Gln Ala Gln 
465                 470                 475                 480 


Tyr Ala Phe Lys Gln Leu Pro Tyr Tyr Gly Lys Ala Leu Gln Arg His 
                485                 490                 495     


Val Ala Phe Gly Ser Gly Asp Ala Lys Asp His Glu Glu Lys Cys Tyr 
            500                 505                 510         


Gly Lys Ile Ala Asn Pro Thr Val His Ile Gly Leu Asn Gln Val Arg 
        515                 520                 525             


Thr Val Val Asn Ala Leu Ile Arg Arg Tyr Gly His Pro Thr Glu Val 
    530                 535                 540                 


Val Val Glu Leu Ala Arg Asp Leu Lys Gln Ser Arg Glu Gln Lys Gln 
545                 550                 555                 560 


Gln Thr Gln Arg Glu Gln Ala Asp Asn Gln Lys Arg Asn Glu Asp Ile 
                565                 570                 575     


Arg Lys Arg Ile Ala Pro Ile Leu Glu Thr Ser Pro Glu Arg Val Arg 
            580                 585                 590         


Asp Arg Asp Ile Lys Lys Trp Ile Leu Trp Glu Glu Leu Asn Lys Lys 
        595                 600                 605             


Asp Ile Ala Asp Arg His Cys Pro Tyr Ser Gly Glu Arg Ile Ser Ala 
    610                 615                 620                 


Thr Met Leu Leu Ser Glu Ala Val Glu Ile Glu His Ile Leu Pro Phe 
625                 630                 635                 640 


Ser Arg Thr Leu Asp Asp Ser Leu Asn Asn Arg Thr Val Ala Met Arg 
                645                 650                 655     


Arg Ala Asn Arg Ile Lys Gly Asp Arg Thr Pro Trp Glu Ala Arg Ala 
            660                 665                 670         


Asp Phe Glu Ala Gln Gly Trp Arg Tyr Glu Ala Ile Leu Gln Arg Ala 
        675                 680                 685             


Glu Gly Met Pro Pro Arg Lys Arg Tyr Arg Phe Ala Glu Asp Gly Tyr 
    690                 695                 700                 


Gln Arg Trp Leu Gly Lys Asp Arg Asn Phe Leu Ala Arg Ala Leu Asn 
705                 710                 715                 720 


Asp Thr Ser Tyr Leu Ser Arg Leu Ala Ala Asn Tyr Leu Arg Leu Val 
                725                 730                 735     


Cys Pro Gln Gly Val Arg Val Ile Pro Gly Gln Met Thr Asp Lys Leu 
            740                 745                 750         


Arg Gly Lys Phe Gly Leu Asn Ser Val Leu Gly Leu Asp Gly Lys Lys 
        755                 760                 765             


Asn Arg Asn Asp His Arg His His Ala Val Asp Ala Cys Val Ile Gly 
    770                 775                 780                 


Val Thr Asp Gln Gly Leu Met Gln Arg Phe Ala Asn Ala Ser Lys Gln 
785                 790                 795                 800 


Ala Arg Glu Asn Gly Leu Thr Arg Leu Val Gln Asp Met Leu Leu Pro 
                805                 810                 815     


Trp Trp Pro Ser Tyr Tyr Asp His Val Glu Arg Ala Val Arg His Ile 
            820                 825                 830         


Arg Val Ser His Arg Pro Asp His Gly Phe Glu Gly Ala Met Met Lys 
        835                 840                 845             


Gly Thr Ala His Gly Ile Arg Glu Asp Gly Ile Arg Glu Asp Gly Arg 
    850                 855                 860                 


Ile Lys Gln Arg Pro Lys Ala Lys Gly Ser Ala Asp His Lys Thr Ile 
865                 870                 875                 880 


Thr Leu Ile Pro Ile Asp Glu Pro Arg Gln Leu Ala Arg His Gly Val 
                885                 890                 895     


Asp Ala Glu Gly Lys Pro Leu Pro Tyr Lys Gly Tyr Ala Ser Gly Ser 
            900                 905                 910         


Asn Tyr Cys Ile Glu Ile Thr Lys Asn Gly Lys Gly Lys Trp Glu Gly 
        915                 920                 925             


Gln Val Ile Ser Thr Phe Asp Ala Tyr Arg Ile Lys Ala Ala Ala Asp 
    930                 935                 940                 


Ala Ala Ala Arg Ala Gly Gln Val Ile Ser Thr Val Glu Ala Asp Ser 
945                 950                 955                 960 


Ile Val Arg Lys Ser Gly Trp Glu Arg Leu Arg Gly Ala Gln Ser Gln 
                965                 970                 975     


Asn Gly Gln Pro Leu Val Met Arg Leu Val Ile Gly Asp Ser Val Arg 
            980                 985                 990         


Met Glu Val Asp Gly Arg Asp Glu  Val Met Arg Val Val  Lys Met Ser 
        995                 1000                 1005             


Gly Lys  Glu Met Val Phe Ala  Pro Val Arg Glu Ala  Asn Val Asp 
    1010                 1015                 1020             


Lys Arg  Asn Asn Met Pro Asp  Glu Gln Asp Pro Phe  Thr Tyr Thr 
    1025                 1030                 1035             


Tyr Lys  Arg Ala Asp Gln Leu  Arg Lys Ala Lys Ala  Arg Gln Val 
    1040                 1045                 1050             


Thr Ile  Ser Pro Ile Gly Glu  Leu Arg Asp Pro Gly  Phe Lys Gly 
    1055                 1060                 1065             


<210>  565
<211>  1068
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  565

Met Gln Tyr Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Cys Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Gln Glu Lys Glu Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Lys Ala Glu Asn Pro Lys Asp Gly Lys Ser Leu Ala Ala 
        35                  40                  45              


Pro Arg Arg Asp Ala Arg Ser Thr Arg Arg Thr Leu Arg Arg Lys Lys 
    50                  55                  60                  


His Arg Met Leu Arg Ile Lys Ile Leu Leu Val Lys Gln Gly Leu Leu 
65                  70                  75                  80  


Ser Lys Asn Glu Leu Asn His Leu Tyr Glu Ser Thr Thr Glu Val Asp 
                85                  90                  95      


Val Trp Asn Leu Arg Leu Asp Ala Leu Glu Arg Lys Leu Asn Pro Lys 
            100                 105                 110         


Glu Phe Thr Arg Val Leu Ile His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Glu Thr Thr Leu Ser Glu Asn Gly Gln Ile Leu Glu 
    130                 135                 140                 


Ser Ile Ser Glu Asn Gln Gln Ile Met Glu Gln Gln Asn Tyr Arg Thr 
145                 150                 155                 160 


Val Gly Glu Met Ile Leu Lys Asp Lys Lys Phe Glu Asn His Lys Arg 
                165                 170                 175     


Asn Lys Asp Gly Thr Tyr Ile Gly Thr Val Thr Arg Gln Gln Leu Gln 
            180                 185                 190         


Glu Glu Ile Gln Met Ile Phe Thr Ala Gln Arg Leu Tyr Lys Asn Asp 
        195                 200                 205             


Tyr Ala Thr Glu Glu Phe Glu Ile Ser Tyr Leu Glu Ile Trp Ala Ser 
    210                 215                 220                 


Gln Arg Pro Tyr Ala Ser Lys Asp Gln Ile Glu Lys Met Ile Gly Tyr 
225                 230                 235                 240 


Cys Thr Leu Glu Pro Lys Glu Lys Arg Val Pro Lys Ala Ser Trp Ser 
                245                 250                 255     


Phe Gln Tyr Phe Val Ala Leu Gln Thr Ile Asn Asn Leu Arg Leu Ile 
            260                 265                 270         


Ser Lys Asp Arg Ile Glu Glu Leu Ser Phe Lys Glu Lys Lys Gln Ile 
        275                 280                 285             


Met Asn Leu Ala Leu Glu Lys Ser Ile Val Lys Tyr Ile Asp Ile Arg 
    290                 295                 300                 


Lys Leu Leu Ser Ile Pro Asn Glu Phe Gln Phe Asn Ser Leu Ser Tyr 
305                 310                 315                 320 


Ser Ala Asp Thr Val Asp Thr Thr Leu Glu Asn Lys Arg Cys Ile Glu 
                325                 330                 335     


Leu Lys Glu Tyr Tyr Ser Ile Asn Lys Val Tyr Lys Gln Ile Tyr Glu 
            340                 345                 350         


Lys Asn Ala Leu Asn Leu Leu Pro Ile Asp Tyr Asp Thr Ile Ala Tyr 
        355                 360                 365             


Gly Leu Thr Ile Phe Lys Asp Asp Lys Asp Ile Leu Glu Tyr Leu Gln 
    370                 375                 380                 


Asn Lys Tyr Val Asn Ala Lys Gly Lys Pro Ile Asn Asn Leu Ala Lys 
385                 390                 395                 400 


Lys Thr Tyr Asp Tyr Thr Phe Ile Gln Ala Leu Leu Thr Leu Asn Phe 
                405                 410                 415     


Thr Lys Met Gly His Leu Ser Phe Lys Ala Leu Lys Asn Ile Ile Pro 
            420                 425                 430         


Phe Leu Glu Glu Gly Leu Ser Tyr Asp Lys Ala Cys Glu Lys Ala Gly 
        435                 440                 445             


Tyr Asn Phe Lys Gly Thr Ser Tyr Thr Glu Lys Thr Lys Tyr Leu Pro 
    450                 455                 460                 


Val Ile Pro Gln Asn Thr Asn Pro Val Val His Arg Ala Leu Ser Gln 
465                 470                 475                 480 


Thr Arg Lys Val Ile Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Asn 
                485                 490                 495     


Ala Ile His Ile Glu Thr Ala Arg Glu Leu Ser Lys Thr Phe Gln Glu 
            500                 505                 510         


Arg Lys Glu Ile Asp Ser Met Tyr Gln Asp Asn Ser Lys Lys Asn Glu 
        515                 520                 525             


His Ala Ile His Lys Leu Lys Glu Leu Gly Asn Ile Asn Pro Ser Gly 
    530                 535                 540                 


Ile Asn Ile Val Lys Phe Lys Leu Trp Asn Glu Gln Asp Gly Arg Cys 
545                 550                 555                 560 


Met Tyr Ser Gly Lys Tyr Ile Glu Pro His Arg Leu Phe Glu Glu Gly 
                565                 570                 575     


Tyr Thr Glu Val Asp His Ile Leu Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Asn Asn Lys Thr Leu Thr Leu Gly Thr Glu Asn Gln Arg Lys 
        595                 600                 605             


Gly Asn Lys Thr Pro Tyr Glu Tyr Met Gly Asn Thr Ser Ile Trp Asn 
    610                 615                 620                 


Asp Phe Glu Thr Arg Val His Ser Asn Lys Lys Ile Asn Lys Lys Lys 
625                 630                 635                 640 


Gln Gln Lys Leu Leu Leu Gln His Phe Ser Tyr Ala Arg Glu Gln Glu 
                645                 650                 655     


Phe Ile Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ala Thr Ile Tyr Leu 
            660                 665                 670         


Ser Ser Leu Ile Gln Gln His Leu Ile Phe Ser Glu Ser Ser Arg Lys 
        675                 680                 685             


Lys Lys Val His Thr Val Ser Gly Ile Ile Thr Ser His Leu Arg Ser 
    690                 695                 700                 


Arg Trp Gly Phe Asn Lys Asp Arg Lys Glu Gly His Ile His His Ala 
705                 710                 715                 720 


Leu Asp Ala Val Ile Val Ala Val Thr Ser Asp His Met Ile Gln Arg 
                725                 730                 735     


Val Thr Lys Tyr Tyr Lys Leu Lys Glu Leu Asn Arg Asn Leu Gln Ala 
            740                 745                 750         


Lys Arg Met Pro Phe Pro Glu Pro Trp Glu Gly Phe Arg Leu Glu Leu 
        755                 760                 765             


Glu Ala Arg Ile Ser Pro Asn Thr Gln Gln Tyr Leu Lys Gly Phe Arg 
    770                 775                 780                 


Phe Lys Asn Tyr Ala Asp Val Asn Leu Ser Glu Ile Lys Pro Ile Phe 
785                 790                 795                 800 


Val Ser Arg Met Pro Lys Arg Ser Ile Thr Gly Glu Leu His Gln Glu 
                805                 810                 815     


Thr Ile Arg Lys Phe Ile Gly Tyr Asn Glu Lys Gly Lys Val Leu Thr 
            820                 825                 830         


Ala Ile Lys Thr Lys Leu Glu Asp Ile Pro Phe Asp Ala Asn Gly Asp 
        835                 840                 845             


Phe Pro Met Tyr Gly Lys Glu Thr Asp Leu Tyr Thr Tyr Asn Ala Ile 
    850                 855                 860                 


Lys Glu Arg Phe Leu Ser His Lys Lys Asp Lys Arg Lys Ser Phe Gln 
865                 870                 875                 880 


Glu Pro Leu Tyr Lys Pro Thr Lys Ser Gly Gly Ile Gly Pro Leu Ile 
                885                 890                 895     


Lys Ser Ile Lys Ile Ile Asp Thr Arg Thr Ile Val Asn Pro Val Asn 
            900                 905                 910         


Gln Gly Lys Gly Val Val Tyr Asn Ser Lys Ile Ala Arg Thr Asp Val 
        915                 920                 925             


Phe Lys Lys Asp Glu Lys Tyr Tyr Leu Ile Pro Ile Tyr Thr Ile Asp 
    930                 935                 940                 


Leu Met Lys Asn Ile Leu Pro Gln Lys Ala Ile Thr Ala Gly Lys Gly 
945                 950                 955                 960 


Tyr Glu Asp Trp Thr Thr Ile Asp Pro Ser Phe Thr Phe Leu Phe Ser 
                965                 970                 975     


Leu Phe Pro Asn Asp Leu Ile Gln Ile Val Pro Ser Lys Asn Lys Thr 
            980                 985                 990         


Ile Lys Ala Arg Thr Thr Val Ser  Lys Lys Glu Val Leu  Leu Pro Ser 
        995                 1000                 1005             


Leu Thr  Gly Tyr Phe Lys Gly  Val His Ser Gly Thr  Ala Gly Ile 
    1010                 1015                 1020             


Thr Val  Glu Thr His Asp Gly  Ser Val Ile Ala Asn  Val Gly Ser 
    1025                 1030                 1035             


Lys Gln  Leu Leu Leu Ile Glu  Lys Tyr Gln Val Asp  Ile Leu Gly 
    1040                 1045                 1050             


His Tyr  Thr Lys Ile Lys Glu  Glu Lys Arg Ile Gly  Met Ala Ile 
    1055                 1060                 1065             


<210>  566
<211>  1068
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  566

Met Pro Lys Ser Ala Lys Pro Gln Gln Ala Tyr Thr Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Ala Ala Leu Leu Gly Gln Gln His Ile 
            20                  25                  30          


Ile Gly Leu His Val Arg Thr Phe Asp Arg Ala Glu Thr Ala Lys Glu 
        35                  40                  45              


Gly Glu Pro Leu Asn Lys Ile Arg Arg Asp Ala Arg Leu Thr Arg Arg 
    50                  55                  60                  


Arg Leu Gln Arg Arg Ala His Arg Leu Leu Arg Leu Arg Arg Leu Phe 
65                  70                  75                  80  


Lys Arg His Asn Leu Ile Pro Asp Thr Asp Thr Ser Thr Phe Ile Ser 
                85                  90                  95      


Gly Gln Ser Pro Trp Glu Leu Arg Ala Gln Gly Leu Asp Arg Gln Leu 
            100                 105                 110         


Thr Ser Ala Glu Trp Thr Thr Val Leu Tyr His Ile Val Lys His Arg 
        115                 120                 125             


Gly Phe His Ser Asn Arg Lys Ser Glu Val Lys Thr Asp Glu Lys Ala 
    130                 135                 140                 


Gly Gln Met Leu Ser Gly Val Asn Gln Asn Gln Ala Leu Leu Lys Gln 
145                 150                 155                 160 


Ser Gly Leu Arg Thr Met Gly Glu Leu Ala Ala Arg His Glu Ala Phe 
                165                 170                 175     


Arg Glu Ala Lys Arg Asn Lys Lys Gly Ala Tyr Asn His Thr Phe Ser 
            180                 185                 190         


Arg His Asp Leu Glu Asp Glu Leu His Leu Leu Phe Ser Arg Gln Arg 
        195                 200                 205             


Glu Leu Gly Asn Pro Tyr Ala Thr Pro Glu Leu Glu Asp Ala Val His 
    210                 215                 220                 


Thr Leu Leu Met Gln Arg Arg Pro Ala Leu Ser Gly Thr Asn Leu Leu 
225                 230                 235                 240 


Lys Met Val Gly His Cys Thr Leu Glu Pro Asp Glu Phe Arg Ala Pro 
                245                 250                 255     


Lys Ala Ser Tyr Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn 
            260                 265                 270         


Asn Leu Arg Val Ser Gly Ser Gly Glu Thr Arg Pro Leu Asn Glu Ser 
        275                 280                 285             


Glu Arg Gln Ala Leu Leu Ser Lys Pro Phe Ser Gln Ser Lys Leu Thr 
    290                 295                 300                 


Tyr Lys Gln Val Arg Lys Ile Leu Glu Leu Pro Asp Asn Ile Arg Phe 
305                 310                 315                 320 


Val Gly Leu His Tyr His Pro Ala Gln Asp Asn Gly Lys Ser Pro Glu 
                325                 330                 335     


Asn Arg Val Phe Phe Glu Ala Lys Ala Phe His Ala Leu Arg Asn Val 
            340                 345                 350         


Tyr Lys Lys Ala Gly Leu Asn Leu Glu Trp Gln Arg Asp Ser Gln Asn 
        355                 360                 365             


Pro Glu Lys Leu Asp Asn Ile Thr Tyr Ala Leu Thr Val Phe Lys Glu 
    370                 375                 380                 


Asp Lys Glu Ala Lys Gln Trp Leu Ile Glu Lys Gly Val Glu Ala Ser 
385                 390                 395                 400 


Val Ile Glu Ala Val Leu Asn Glu Ser Phe Ser Glu Phe Val Arg Leu 
                405                 410                 415     


Ser Ile Lys Ala Leu Arg Lys Ile Ile Pro His Met Glu Ile Gly His 
            420                 425                 430         


Arg Tyr Asp Glu Ala Val Lys Leu Ala Gly Tyr Ser His His Ser His 
        435                 440                 445             


Leu Pro Gln Pro Ser Lys Thr Pro Tyr Ile Pro Arg Phe Ser Lys Gln 
    450                 455                 460                 


Ser Ile Ala Asn Pro Val Val Ser Arg Ala Leu Asn Gln Ala Arg Lys 
465                 470                 475                 480 


Leu Val Asn Ala Ile Val Arg Glu Tyr Gly Pro Pro Asp Ala Val His 
                485                 490                 495     


Ile Glu Leu Ala Arg Asp Leu Asn Lys Pro Phe Asp Glu Arg Arg Lys 
            500                 505                 510         


Ile Glu Lys Asp Gln Glu Lys Tyr Gln Lys Asp Lys Ala Arg Asp Ile 
        515                 520                 525             


Glu Thr Phe Gln Glu Asn Phe Gly Phe Thr Pro Lys Gly Leu Gln Leu 
    530                 535                 540                 


Thr Lys Trp Arg Leu Tyr Arg Glu Gln Ser Gly Lys Cys Ala Tyr Ser 
545                 550                 555                 560 


Leu Lys Pro Leu Asp Ile Asn Arg Leu Phe Glu Asp Gly Tyr Val Glu 
                565                 570                 575     


Ile Asp His Ala Leu Pro Tyr Ser Arg Ser Phe Asn Asp Gly Met Asn 
            580                 585                 590         


Asn Lys Val Leu Thr Leu Thr Thr Glu Asn Arg Asn Lys Gly Asn Gln 
        595                 600                 605             


Thr Pro Tyr Glu Tyr Leu Asp Gly Ala Ser Asn Ser Ala Gln Trp Arg 
    610                 615                 620                 


Asn Phe Val Ala Trp Val Asn Ser Asn Lys Ser Tyr Arg Ala Ser Lys 
625                 630                 635                 640 


Gln Arg Asn Leu Leu Arg Lys Asp Phe Ala Ala Gln Ala Ala Thr Glu 
                645                 650                 655     


Phe Arg Glu Arg His Leu Thr Asp Thr Arg Tyr Ile Ala Arg Glu Phe 
            660                 665                 670         


Lys Arg Met Val Glu Thr His Leu Lys Leu Asn Lys Pro Asn Gln Arg 
        675                 680                 685             


Cys Val Val Val Ser Gly Gln Leu Thr Ala Tyr Leu Arg Ala Arg Trp 
    690                 695                 700                 


Gly Leu Ile Lys Val Arg Glu Asn Gly Asp Leu His His Ala Leu Asp 
705                 710                 715                 720 


Ala Ala Val Val Ala Ala Cys Ser His Ala Met Val Lys Arg Leu Ala 
                725                 730                 735     


Asp Tyr Ser Arg Arg Gly Glu Leu Gln Tyr Ala Arg Gly His His Phe 
            740                 745                 750         


Asp Pro Glu Thr Gly Glu Ile Ile Asp Ile Asn Ala Leu Arg Lys Leu 
        755                 760                 765             


Asp Asp His Phe Pro Gln Pro Trp Thr Asp Phe Arg Arg Glu Leu Leu 
    770                 775                 780                 


Ala Trp Leu Ser Pro Asp Pro Ala Gln Gln Leu Lys Asn Leu Ala Tyr 
785                 790                 795                 800 


Tyr Thr Asp Glu Arg Leu Lys Gln Val Lys Pro Ile Arg Val Ser Arg 
                805                 810                 815     


Ala Pro Thr Arg Arg Gly Leu Gly Ala Ala His Gln Glu Thr Ile Arg 
            820                 825                 830         


Ser Ala Lys Tyr Leu Gly Glu Asn Arg Ser Thr Val Lys Thr Pro Leu 
        835                 840                 845             


Glu Lys Leu Lys Leu Lys Asp Ile Pro Asn Ile Val Gly Tyr Glu Asp 
    850                 855                 860                 


Pro Arg Asn His Gln Leu Ile Gln Ala Ile Ser Gln Arg Leu Thr Glu 
865                 870                 875                 880 


His Gly Asp Asp Gly Lys Lys Ala Phe Lys Glu Pro Leu Tyr Lys Pro 
                885                 890                 895     


Gly Asn Asn Lys Ser Thr Ala Pro Val Val Arg Ser Val Lys Leu Phe 
            900                 905                 910         


Thr Val Gln Lys Ser Gly Ile Pro Val Arg Gly Gly Ile Ala Asn Asn 
        915                 920                 925             


Gly Asn Met Val Arg Val Asp Ile Phe Thr Lys Lys Glu Arg Tyr Phe 
    930                 935                 940                 


Ala Val Pro Val Tyr Val Ser Asp Ala Ala Arg Asn Glu Leu Pro Asn 
945                 950                 955                 960 


Lys Ala Val Ala Arg Gly Lys Asp Gly Trp Ile Asp Ile Asp Asp Ser 
                965                 970                 975     


Phe Ser Phe Leu Phe Ser Leu Tyr Pro Asn Asp Trp Val Ser Val Val 
            980                 985                 990         


Val Lys Pro His Glu Ala Pro Cys  Glu Gly Tyr Tyr Ala  Gly Leu Asp 
        995                 1000                 1005             


Ile Ser  Thr Gly Ala Ile His  Ile Trp Ala His Asp  Arg Asn Arg 
    1010                 1015                 1020             


Ser Val  Gly Lys Asn Gly Leu  Met Arg Ser Ile Gly  Ile Lys Thr 
    1025                 1030                 1035             


Ala His  Ser Val Glu Lys Tyr  His Val Asp Leu Leu  Gly Asn Leu 
    1040                 1045                 1050             


Tyr Arg  Val Val His Glu Thr  Arg Lys Pro Leu Arg  Ala Gly Lys 
    1055                 1060                 1065             


<210>  567
<211>  1068
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  567

Met Gln Tyr Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Cys Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Gln Glu Lys Glu Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Lys Ala Glu Asn Pro Lys Asp Gly Lys Ser Met Ala Thr 
        35                  40                  45              


Pro Arg Arg Asp Ala Arg Ser Thr Arg Arg Thr Leu Arg Arg Lys Lys 
    50                  55                  60                  


His Arg Met Gln Arg Ile Lys Ile Ile Leu Val Lys Gln Gly Leu Leu 
65                  70                  75                  80  


Ser Lys Thr Glu Leu Asp His Leu Tyr Glu Ser Ala Thr Glu Val Asp 
                85                  90                  95      


Val Trp Asn Leu Arg Leu Asp Ala Leu Glu Arg Lys Leu Asn Pro Lys 
            100                 105                 110         


Glu Phe Thr Arg Val Leu Ile His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Glu Thr Thr Leu Ser Glu Asn Gly Gln Ile Leu Glu 
    130                 135                 140                 


Ser Ile Ser Glu Asn Gln Gln Ile Met Gly Gln Lys Asn Tyr Arg Thr 
145                 150                 155                 160 


Val Gly Glu Met Ile Leu Gln Asp Lys Lys Phe Glu Ser His Lys Arg 
                165                 170                 175     


Asn Lys Asp Gly Thr Tyr Ile Gly Thr Val Thr Arg Gln Gln Leu Gln 
            180                 185                 190         


Asp Glu Ile Gln Met Ile Phe Asn Ala Gln Arg Leu Tyr Lys Asn Asn 
        195                 200                 205             


Tyr Ala Thr Ala Glu Phe Glu Asn Ser Tyr Leu Glu Ile Trp Ala Ser 
    210                 215                 220                 


Gln Arg Pro Tyr Ala Ser Lys Asp Gln Ile Glu Lys Met Ile Gly Tyr 
225                 230                 235                 240 


Cys Thr Leu Glu Gln Lys Glu Lys Arg Ala Pro Lys Ala Ser Trp Ser 
                245                 250                 255     


Phe Gln Tyr Phe Val Ala Leu Gln Thr Ile Asn Asn Leu Arg Leu Ile 
            260                 265                 270         


Ser Lys Asp Arg Ile Glu Glu Leu Ser Phe Glu Glu Lys Lys Gln Ile 
        275                 280                 285             


Met Asn Leu Ala Leu Glu Lys Ser Ile Val Lys Tyr Ile Asp Ile Arg 
    290                 295                 300                 


Lys Leu Leu Ser Val Pro Asn Glu Phe Gln Phe Asn Ser Leu Ser Tyr 
305                 310                 315                 320 


Ser Ala Asp Thr Val Asp Ile Thr Val Glu Ser Arg Lys Cys Ile Glu 
                325                 330                 335     


Leu Lys Glu Tyr His Ser Ile Asn Lys Val Tyr Lys Gln Ile Tyr Glu 
            340                 345                 350         


Lys Asn Ala Leu Asn Leu Leu Pro Lys Asp Tyr Asp Thr Ile Ala Tyr 
        355                 360                 365             


Gly Leu Thr Ile Phe Lys Asp Asp Lys Asp Ile Leu Glu Tyr Leu Gln 
    370                 375                 380                 


Asn Lys Tyr Val Asn Ala Lys Gly Lys Pro Ile Ser Asn Phe Ala Asn 
385                 390                 395                 400 


Lys Thr Tyr Asp Asp Thr Phe Ile Gln Ala Leu Leu Asn Leu Asn Phe 
                405                 410                 415     


Thr Lys Met Gly His Leu Ser Phe Lys Ala Leu Lys Asn Ile Ile Pro 
            420                 425                 430         


Phe Leu Glu Glu Gly Leu Ser Tyr Asp Lys Ala Cys Glu Lys Ala Gly 
        435                 440                 445             


Tyr Asn Phe Lys Gly Thr Ser Tyr Thr Glu Lys Thr Lys Tyr Leu Pro 
    450                 455                 460                 


Val Ile Pro Gln Asn Thr Asn Pro Val Val His Arg Ala Leu Ser Gln 
465                 470                 475                 480 


Thr Arg Lys Val Ile Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Asn 
                485                 490                 495     


Ala Ile His Ile Glu Thr Ala Arg Glu Leu Ser Lys Thr Phe Gln Glu 
            500                 505                 510         


Arg Lys Glu Ile Asp Ser Met Tyr Gln Asp Asn Ser Lys Lys Asn Glu 
        515                 520                 525             


His Ala Ile Cys Lys Leu Lys Glu Leu Gly Asn Ile Asn Pro Ser Gly 
    530                 535                 540                 


Ile Asn Ile Val Lys Phe Lys Leu Trp Asn Glu Gln Asp Gly Arg Cys 
545                 550                 555                 560 


Met Tyr Ser Gly Lys Tyr Ile Glu Pro His Arg Leu Phe Glu Glu Gly 
                565                 570                 575     


Tyr Thr Glu Val Asp His Ile Leu Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Asn Asn Lys Thr Leu Thr Leu Gly Thr Glu Asn Gln Arg Lys 
        595                 600                 605             


Gly Asn Lys Thr Pro Tyr Glu Tyr Met Gly Asn Thr Ser Ile Trp Asn 
    610                 615                 620                 


Asp Phe Glu Thr Arg Val Gln Ser Asn Lys Lys Ile Thr Lys Lys Lys 
625                 630                 635                 640 


Gln Gln Lys Leu Leu Leu Gln His Phe Ser Tyr Ala Arg Glu Gln Glu 
                645                 650                 655     


Phe Ile Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ala Thr Ile Tyr Leu 
            660                 665                 670         


Ser Ser Leu Ile Gln Gln His Leu Ile Phe Ser Glu Ser Ser Arg Lys 
        675                 680                 685             


Lys Lys Val His Thr Val Ser Gly Ile Ile Thr Ser His Leu Arg Ser 
    690                 695                 700                 


Arg Trp Gly Phe Asn Lys Asp Arg Lys Glu Gly His Ile His His Ala 
705                 710                 715                 720 


Leu Asp Ala Val Ile Val Ala Val Thr Ser Asp His Met Ile Gln Arg 
                725                 730                 735     


Val Thr Lys Tyr Tyr Lys Leu Lys Glu Leu Asn Arg Asn Leu Gln Ala 
            740                 745                 750         


Lys Arg Met Pro Phe Pro Glu Pro Trp Glu Gly Phe Arg Leu Glu Leu 
        755                 760                 765             


Glu Ala Arg Ile Ser Pro Asn Thr Gln Gln Tyr Leu Lys Gly Phe Arg 
    770                 775                 780                 


Phe Lys Asn Tyr Ala Asp Val Asn Leu Ser Glu Ile Lys Pro Ile Phe 
785                 790                 795                 800 


Val Ser Arg Met Pro Lys Arg Ser Ile Thr Gly Glu Leu His Gln Glu 
                805                 810                 815     


Thr Ile Arg Lys Phe Ile Gly Tyr Asn Glu Lys Gly Lys Val Leu Thr 
            820                 825                 830         


Ala Ile Lys Thr Lys Leu Glu Asp Ile Pro Phe Asp Ala Asn Gly Asp 
        835                 840                 845             


Phe Pro Met Tyr Gly Lys Glu Thr Asp Leu Tyr Thr Tyr Asn Ala Ile 
    850                 855                 860                 


Lys Glu Arg Phe Leu Ser His Lys Lys Asp Lys Arg Lys Ser Phe Gln 
865                 870                 875                 880 


Glu Pro Leu Tyr Lys Pro Thr Lys Ser Gly Gly Ile Gly Pro Leu Ile 
                885                 890                 895     


Lys Ser Ile Lys Ile Ile Asp Thr Arg Thr Ile Val Asn Pro Val Asn 
            900                 905                 910         


Gln Gly Lys Gly Val Val Tyr Asn Ser Lys Ile Ala Arg Thr Asp Val 
        915                 920                 925             


Phe Lys Lys Asp Glu Lys Tyr Tyr Leu Ile Pro Ile Tyr Thr Ile Asp 
    930                 935                 940                 


Leu Met Lys Asn Ile Leu Pro Gln Lys Ala Ile Thr Ala Gly Lys Gly 
945                 950                 955                 960 


Tyr Glu Asp Trp Thr Thr Ile Asp Pro Ser Phe Thr Phe Leu Phe Ser 
                965                 970                 975     


Leu Phe Pro Asn Asp Leu Ile Gln Ile Val Pro Ser Lys Asn Lys Thr 
            980                 985                 990         


Ile Lys Ala Arg Thr Thr Val Ser  Lys Lys Glu Val Leu  Leu Pro Ser 
        995                 1000                 1005             


Leu Thr  Gly Tyr Phe Lys Gly  Val His Ser Gly Thr  Ala Gly Ile 
    1010                 1015                 1020             


Thr Val  Glu Thr His Asp Gly  Ser Val Ile Ala Asn  Val Gly Ser 
    1025                 1030                 1035             


Lys Gln  Leu Leu Leu Phe Glu  Lys Tyr Gln Val Asp  Val Leu Gly 
    1040                 1045                 1050             


His Tyr  Thr Lys Ile Lys Glu  Glu Lys Arg Ile Gly  Met Ala Ile 
    1055                 1060                 1065             


<210>  568
<211>  1069
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  568

Met Lys Lys Gln Pro Gln Arg Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ala Leu Ile Leu Val Asp Gly Ala Glu Asn Pro 
            20                  25                  30          


Val Gly Leu Leu Asp Cys Gly Val Arg Thr Phe Glu Arg Ala Glu Val 
        35                  40                  45              


Pro Lys Thr Gly Asp Ser Leu Ala Leu Ala Arg Arg Gln Ala Arg Ser 
    50                  55                  60                  


Val Arg Arg Leu Ile Arg Arg Arg Ala His Arg Leu Leu Arg Leu Arg 
65                  70                  75                  80  


Arg Leu Leu Lys Arg Glu Gly Ile Leu Ser Ala Ala Asp Phe Asn Ser 
                85                  90                  95      


Asp Gly Leu Pro Asn Leu Pro Ile Asp Ala Trp Gln Leu Arg Val Ala 
            100                 105                 110         


Gly Leu Glu Arg Lys Leu Thr Asn Gln Glu Trp Ala Ala Val Leu Leu 
        115                 120                 125             


His Leu Val Lys His Arg Gly Tyr Leu Ser Gln Arg Lys Ser Glu Thr 
    130                 135                 140                 


Gln Thr Glu Asn Lys Glu Leu Gly Arg Leu Leu Ala Gly Val Ala Glu 
145                 150                 155                 160 


Asn Ser Lys Leu Leu Ser Glu Ser Ser Glu Gln Tyr Arg Thr Pro Ala 
                165                 170                 175     


Glu Leu Ala Val Lys Leu Phe Ala Glu Gln Ser Gly Arg Leu Arg Asn 
            180                 185                 190         


Lys Gly Gly Asp Tyr Thr His Thr Phe Asn Arg Leu Asp Leu Gln Lys 
        195                 200                 205             


Glu Leu His Leu Leu Phe Glu Arg Gln Cys Ala Leu Gly Asn Pro Phe 
    210                 215                 220                 


Ala Ser Ala Glu Leu Gln Ser Lys Val Asp Glu Leu Leu Met Thr Gln 
225                 230                 235                 240 


Arg Ser Ala Leu Gln Gly Glu Ala Val Leu Lys Met Leu Gly Lys Cys 
                245                 250                 255     


Thr Phe Glu Pro Ser Glu Tyr Lys Ala Ala Lys Asn Thr Tyr Ser Ala 
            260                 265                 270         


Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile Leu Tyr 
        275                 280                 285             


Asn Gly Glu Asp Arg Ala Leu Asp Glu Gly Glu Arg Lys Gln Leu Leu 
    290                 295                 300                 


Asp Glu Pro Tyr Lys Lys Ala Lys Leu Thr Tyr Lys Gln Val Arg Ser 
305                 310                 315                 320 


Ile Leu Asn Leu Pro Glu Ser Ala Val Phe Lys Gly Leu Arg Tyr Gly 
                325                 330                 335     


Lys Asp Ser Asp Gly Leu Lys Ala Glu Asn Gly Ala Leu Met Glu Met 
            340                 345                 350         


Lys Ala Tyr His Lys Ile Arg Thr Val Leu Glu Lys Ala Gly Leu Lys 
        355                 360                 365             


Gly Glu Trp Glu Asn Arg Ile Arg Asn Pro Glu Ile Leu Asp Gln Ile 
    370                 375                 380                 


Gly Thr Ala Phe Ser Val Tyr Lys Thr Asp Glu Asp Ile Lys Ala Glu 
385                 390                 395                 400 


Leu Ala Ala Leu Arg Leu Pro Glu Asn Val Leu Asp Ala Leu Leu Ala 
                405                 410                 415     


Gly Leu Asn Phe Asp Lys Phe Ile Gln Leu Ser Leu Lys Ala Leu Ser 
            420                 425                 430         


Asn Ile Leu Pro Leu Met Glu Arg Gly Ile Arg Tyr Asp Glu Ala Cys 
        435                 440                 445             


Lys Glu Val Tyr Gly Asp His Arg Ala Leu Ala Glu Ser Lys Asn Gln 
    450                 455                 460                 


Met Leu Leu Pro Gln Ile Pro Ala Asn Glu Ile Arg Asn Pro Val Val 
465                 470                 475                 480 


Leu Arg Thr Leu Thr Gln Ala Arg Lys Val Ile Asn Ala Ile Ile Arg 
                485                 490                 495     


Arg Tyr Gly Ser Pro Gln Arg Val His Ile Glu Thr Ala Arg Glu Leu 
            500                 505                 510         


Gly Lys Ser Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu 
        515                 520                 525             


Asn Arg Lys Glu Arg Glu Arg Ala Ala Glu His Phe Lys Glu Leu Phe 
    530                 535                 540                 


Pro Arg Phe Thr Gly Glu Pro Lys Ser Gly Asp Ile Leu Lys Leu Arg 
545                 550                 555                 560 


Leu Tyr Gln Gln Gln Gln Gly Lys Cys Leu Tyr Ser Gly Ile Pro Ile 
                565                 570                 575     


Asp Leu Gly Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala 
            580                 585                 590         


Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu 
        595                 600                 605             


Val Leu Gly Ser Glu Asn Gln Lys Lys Arg Asn Gln Thr Pro Tyr Glu 
    610                 615                 620                 


Tyr Leu Asp Gly Ala Arg Asp Ser Gln Arg Trp Arg Glu Phe Gln Ala 
625                 630                 635                 640 


Arg Val Gln Gly Cys Arg Phe Pro Tyr Ala Lys Lys Gln Arg Ile Gln 
                645                 650                 655     


Thr Ala Lys Leu Asp Ser Lys Thr Glu Gln Gly Phe Leu Glu Arg Asn 
            660                 665                 670         


Leu Asn Asp Thr Arg Tyr Ile Ala Arg Phe Leu Cys Gly Phe Ile Glu 
        675                 680                 685             


Lys Ser Leu His Leu Gln Gly Ala Gly Lys Arg Arg Val Phe Ala Ser 
    690                 695                 700                 


Asn Gly Gln Ile Thr Ala Leu Leu Arg Gly Leu Trp Gly Leu Lys Lys 
705                 710                 715                 720 


Val Arg Glu Asp Asn Asp Arg His His Ala Leu Asp Ala Ile Val Val 
                725                 730                 735     


Ala Cys Ser Thr Val Ser Met Gln Gln Lys Ile Thr Lys Ala Met Gln 
            740                 745                 750         


Arg Arg Glu Thr Leu Glu Thr Ile Asn Thr Glu Thr Gly Glu Val Lys 
        755                 760                 765             


Thr Arg Ile Pro Gln Pro Trp Asp Phe Phe Arg Gln Glu Val Met Ile 
    770                 775                 780                 


Arg Val Phe Ser Asp Ala Pro Arg Glu Glu Leu Ala Glu Lys Leu Asn 
785                 790                 795                 800 


Ser Arg Pro Glu Ala Leu His Asp Phe Val Ser Pro Leu Phe Val Ser 
                805                 810                 815     


Arg Ala Pro Asn Arg Lys Met Thr Gly Gln Gly His Leu Glu Thr Val 
            820                 825                 830         


Lys Ser Ala Lys Arg Leu Ser Glu Asn Ile Ser Val Val Lys Lys Pro 
        835                 840                 845             


Leu Thr Ala Leu Lys Leu Lys Asp Ile Glu Asn Ile Val Gly Tyr Pro 
    850                 855                 860                 


Asp Arg Glu Pro Ala Leu Tyr Arg Ala Leu Gln Glu Arg Leu Gln Ala 
865                 870                 875                 880 


Tyr Lys Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Pro 
                885                 890                 895     


Ala Lys Asp Gly Gly Gln Gly Ser Leu Val Lys Ala Val Arg Ile Ile 
            900                 905                 910         


Glu Asn Thr Lys His Gly Leu Lys Val Arg Asn Gly Ile Thr Asp Thr 
        915                 920                 925             


Asn Gly Asp Met Val Arg Ala Asp Val Tyr Gln Lys Ala Asp Lys Lys 
    930                 935                 940                 


Gly Lys Arg Gln Tyr Phe Ile Val Pro Val Tyr Ala Trp Gln Val Glu 
945                 950                 955                 960 


Lys Asn Glu Leu Pro Thr Val Asp Cys Lys Gly Tyr Glu Ile Asp Asp 
                965                 970                 975     


Thr Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Tyr Asp Ile Ile Glu Ile 
            980                 985                 990         


Gln Asn Pro Asp Asn Met Ser Lys  Glu Leu Arg Val Tyr  Tyr Ile Asn 
        995                 1000                 1005             


Cys Asp  Ser Ser Asp Gly Arg  Phe Trp Leu Ala His  His Asp Lys 
    1010                 1015                 1020             


Ser Ala  Lys Glu Gln Gln Phe  Arg Ile Ser Thr Gln  Asn Gln Pro 
    1025                 1030                 1035             


Ile Ile  Lys Lys Tyr Gln Val  Asp Pro Leu Gly Lys  Glu Ile Arg 
    1040                 1045                 1050             


Leu Cys  Lys Ala Glu Lys Arg  Thr Glu Leu Asn Gly  Lys Lys Lys 
    1055                 1060                 1065             


Lys 
    


<210>  569
<211>  1069
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  569

Met Asn Tyr Asp Ile Gly Leu Arg Ile Gly Ile Thr Ser Cys Gly Trp 
1               5                   10                  15      


Ser Ile Ile Asn Lys Asp Leu Glu Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Val Phe Glu Lys Ala Glu Asn Pro Asp Gly Thr Ala Ser Ala Ala Pro 
        35                  40                  45              


Arg Arg Glu Ala Arg Lys Ser Arg Arg Lys His Arg Arg Lys Lys His 
    50                  55                  60                  


Arg Ile Glu Arg Ile Lys Arg Leu Ile Val Gln His Asp Leu Leu Ser 
65                  70                  75                  80  


Lys Lys Glu Leu Asp Ser Leu Tyr Leu Ser Pro Phe Asn Ile Asp Val 
                85                  90                  95      


Trp Asn Leu Arg Val Glu Ala Leu Glu Arg Lys Leu Asp Asn Arg Glu 
            100                 105                 110         


Phe Ala Arg Val Leu Ile His Leu Val Gln Arg Arg Gly Phe Gln Thr 
        115                 120                 125             


Ile Arg Lys Ser Val Glu Ile Gln Glu Glu Gly Lys Leu Leu Glu Asn 
    130                 135                 140                 


Val Ser Glu Asn Glu Arg Ile Met Lys Val Asn Gly Tyr Lys Thr Val 
145                 150                 155                 160 


Gly Glu Met Phe Ser Lys His Glu Lys Phe Thr His His Lys Arg Asn 
                165                 170                 175     


Lys Asp Gly Ser Tyr Thr Ser Val Val Thr Arg Ser Leu Leu Leu Lys 
            180                 185                 190         


Glu Ile Lys Thr Ile Phe Asn Ser Gln Arg Ile Leu Gly Asn Val Phe 
        195                 200                 205             


Ser Thr Gln Lys Leu Glu Ser Asp Tyr Leu Tyr Ile Trp Gly Ser Gln 
    210                 215                 220                 


Arg Pro Thr Leu Thr Tyr Glu Gln Leu Met Ser Met Val Gly Asn Cys 
225                 230                 235                 240 


Ile Tyr Glu Lys Lys Glu Lys Arg Ala Pro Lys Thr Ser Trp Ala Phe 
                245                 250                 255     


Gln Tyr Phe Ser Leu Leu Gln Lys Ile Asn Lys Leu Lys Val Ser Asp 
            260                 265                 270         


Asp Asn Thr Leu Arg Lys Leu Ser Lys Glu Glu Arg Asp Ile Val Ile 
        275                 280                 285             


Glu Leu Ala Phe Lys Lys Lys Asn Val Ser Phe Val Asp Ile Arg Lys 
    290                 295                 300                 


Ala Leu Lys Leu Asn Asp Asn Thr Lys Phe Asn His Leu Thr Tyr Ser 
305                 310                 315                 320 


His Val Val Glu Thr Lys Lys Val Glu Lys Ala Thr Phe Ile Glu Leu 
                325                 330                 335     


Lys Gly Tyr His Leu Ile Lys Lys Gln Leu Lys Asp Arg Asn Val Glu 
            340                 345                 350         


Leu Thr Gln Glu Leu Gln Pro Lys Asp Tyr Asp Ala Ile Ala Ala Ala 
        355                 360                 365             


Ser Thr Phe Phe Lys Asn Asp Ile Glu Phe Arg Asp Tyr Leu Arg Asn 
    370                 375                 380                 


Gln Tyr Leu Asp Ser Asn Gly Lys Arg Lys Ser Asn Ile Ala Asn Lys 
385                 390                 395                 400 


Val Phe Glu Asp Lys Val Ile Ala Ala Val Phe Glu Leu Asn Phe Ser 
                405                 410                 415     


Lys Val Cys His Leu Ser Phe Lys Ser Leu Tyr Lys Leu Ile Pro His 
            420                 425                 430         


Leu Glu Lys Gly Leu Asp Tyr Lys Glu Ala Ile Leu Cys Ala Gly Tyr 
        435                 440                 445             


Asn Phe Arg Asp Glu Asn Lys Arg Asp Lys Lys Leu Phe Leu Pro Val 
    450                 455                 460                 


Ile Gln Gln Glu Thr Asn Pro Val Val His Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Ile Asn Ala Ile Ile Arg Glu Tyr Gly Ser Pro Asn Thr 
                485                 490                 495     


Ile Arg Phe Val Val Ala Asn Asp Leu Ala Lys Thr Tyr Lys Gln Arg 
            500                 505                 510         


Lys Glu Leu Glu Ser Thr Leu Lys Lys Asn Arg Ala Glu Asn Glu Lys 
        515                 520                 525             


Ile Lys Thr Lys Leu Leu Ser Met Gly Ile Ile Asn Pro Thr Gly Met 
    530                 535                 540                 


Asp Ile Val Lys Tyr Lys Leu Trp Tyr Glu Gln Ser Gly Ile Cys Leu 
545                 550                 555                 560 


Tyr Thr Gly Arg Gln Met Glu Leu Ser Ser Leu Phe Lys Pro Gly Tyr 
                565                 570                 575     


Ser Ile Val Asn His Ile Ile Pro Tyr Asn Arg Ser Phe Asp Asp Thr 
            580                 585                 590         


Tyr His Asn Arg Val Leu Thr Leu Thr Glu Thr Lys Tyr Glu Lys Gly 
        595                 600                 605             


Asn Lys Ile Pro Phe Glu Tyr Phe Gly Ala Glu Glu Thr Cys Trp Asn 
    610                 615                 620                 


Glu Tyr Glu Lys Arg Val Ser Thr Ser Lys Lys Ile His Asp Lys Lys 
625                 630                 635                 640 


Arg Glu Lys Leu Leu Ile Arg Asn Phe Asp Ile Glu Tyr Glu Asn Glu 
                645                 650                 655     


Met Ile Ser Asn Asn Val Phe Asp Thr His Tyr Ile Gly Ser Tyr Phe 
            660                 665                 670         


Glu Lys Tyr Ile Asp Glu His Leu Gln Phe Lys Lys Ser Thr Lys Lys 
        675                 680                 685             


Gln Lys Val Phe Ser Ile His Gly Asn Met Val Ala His Leu His Ser 
    690                 695                 700                 


Arg Trp Gly Phe Asn Lys Asn Ser Arg Glu Ile Tyr Leu Asn His Ala 
705                 710                 715                 720 


Val Asp Ala Ala Ile Val Ala Val Leu Thr Asp Gln Leu Ile Arg Asn 
                725                 730                 735     


Ile Ala Asp Tyr Tyr Arg Arg Asp Asn Ile Leu Glu Gln Lys Gly Ser 
            740                 745                 750         


Leu Lys Phe Pro Glu Pro Trp Lys Ser Phe Ser Ile Glu Leu Glu Ala 
        755                 760                 765             


Arg Ile Ser Pro Gln Val Arg Asp Leu Ile Lys Lys Leu Asn Leu Asp 
    770                 775                 780                 


Ser Tyr Leu Asp Val Asp Ile Leu His Ile Lys Pro Val Phe Ile Ser 
785                 790                 795                 800 


Lys Lys Pro Arg Arg Lys Val Asn Gly Glu Ala His Lys Ser Thr Ile 
                805                 810                 815     


Arg Ser Leu Ile Gly Ile Asn Glu Ile Gly Lys Thr Val Thr Cys Ile 
            820                 825                 830         


Lys Thr Arg Leu Glu Asp Ile Pro Phe Asp Glu Asn Gly Asp Phe Pro 
        835                 840                 845             


Met Tyr Gly Lys Asp Thr Asp Lys Tyr Thr Tyr Thr Ala Ile Lys Glu 
    850                 855                 860                 


Arg Tyr Leu Glu Phe Asn Lys Asp Lys Glu Lys Ala Phe Ser Thr Pro 
865                 870                 875                 880 


Leu Tyr Lys Leu Lys Arg Asn Gly Glu Pro Gly Asn Gln Ile Lys Ser 
                885                 890                 895     


Val Lys Ile Ile Asp Thr Arg Asn Ile Val Asn Lys Val Asn Gln Gly 
            900                 905                 910         


Lys Gly Ile Ala Tyr Asn Ser Asn Ile Ile Arg Val Asp Val Phe Ser 
        915                 920                 925             


Arg Asp Gln Lys Tyr Tyr Val Val Pro Ile Tyr Leu Arg Asp Phe Ile 
    930                 935                 940                 


Lys Gly Ser Leu Pro Asn Lys Ile Ile Thr Ala Ala Lys Gly Phe Met 
945                 950                 955                 960 


Gln Trp Pro Glu Thr Asp Asn Ser Tyr Thr Phe Leu Phe Ser Ile Phe 
                965                 970                 975     


Pro Asn Asp Leu Ile Lys Val Val Pro Lys Glu Gly Lys Phe Ile Lys 
            980                 985                 990         


Ala Lys Asn Glu Asn Lys Glu Asn  Ile Asn Leu Thr Glu  Ile Leu Gly 
        995                 1000                 1005             


Tyr Phe  Lys Gly Leu Asp Ser  Ser Thr Gly Ala Phe  Thr Leu Glu 
    1010                 1015                 1020             


Ser His  Asp Gly Ser Leu Leu  Ala Arg Gly Ile Gly  Thr Lys Asn 
    1025                 1030                 1035             


Leu Leu  Leu Ile Glu Lys Tyr  Gln Val Asp Val Leu  Gly Arg Tyr 
    1040                 1045                 1050             


Asn Lys  Val Gly Asn Glu Glu  Arg Gln Asp Ile Lys  Ile Gln Gln 
    1055                 1060                 1065             


Asn 
    


<210>  570
<211>  1069
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  570

Met Asn Tyr Asp Ile Gly Leu Arg Ile Gly Ile Thr Ser Cys Gly Trp 
1               5                   10                  15      


Ser Ile Ile Asn Lys Asp Leu Glu Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Val Phe Glu Lys Ala Glu Asn Pro Asp Gly Thr Ala Ser Ala Ala Pro 
        35                  40                  45              


Arg Arg Glu Ala Arg Lys Ser Arg Arg Lys His Arg Arg Lys Lys His 
    50                  55                  60                  


Arg Ile Glu Arg Ile Lys Arg Leu Ile Val Gln His Asp Leu Leu Ser 
65                  70                  75                  80  


Lys Lys Glu Leu Asp Ser Leu Tyr Leu Ser Pro Phe Asn Ile Asp Val 
                85                  90                  95      


Trp Asn Leu Gln Val Glu Ala Leu Glu Arg Lys Leu Asp Asn Arg Glu 
            100                 105                 110         


Phe Ala Arg Val Leu Ile His Leu Val Gln Arg Arg Gly Phe Gln Thr 
        115                 120                 125             


Ile Arg Lys Ser Val Glu Ile Gln Glu Glu Gly Lys Leu Leu Glu Asn 
    130                 135                 140                 


Val Ser Glu Asn Glu Arg Ile Met Lys Val Asn Gly Tyr Lys Thr Val 
145                 150                 155                 160 


Gly Glu Met Phe Ser Lys His Glu Lys Phe Thr His His Lys Arg Asn 
                165                 170                 175     


Lys Asp Gly Ser Tyr Thr Ser Val Val Thr Arg Ser Leu Leu Leu Lys 
            180                 185                 190         


Glu Ile Lys Thr Ile Phe Asn Ser Gln Arg Ile Leu Gly Asn Val Phe 
        195                 200                 205             


Ser Thr Gln Lys Leu Glu Ser Asp Tyr Leu Tyr Ile Trp Gly Ser Gln 
    210                 215                 220                 


Arg Pro Thr Leu Thr Tyr Glu Gln Leu Met Ser Met Val Gly Asn Cys 
225                 230                 235                 240 


Ile Tyr Glu Lys Lys Glu Lys Arg Ala Pro Lys Thr Ser Trp Ala Phe 
                245                 250                 255     


Gln Tyr Phe Ser Leu Leu Gln Lys Ile Asn Lys Leu Lys Val Ser Asp 
            260                 265                 270         


Asp Asn Thr Leu Arg Lys Leu Ser Lys Glu Glu Arg Asp Ile Val Ile 
        275                 280                 285             


Glu Leu Ala Phe Lys Lys Lys Asn Val Ser Phe Val Asp Ile Arg Lys 
    290                 295                 300                 


Ala Leu Lys Leu Asn Asp Asn Thr Lys Phe Asn His Leu Thr Tyr Ser 
305                 310                 315                 320 


His Val Val Glu Thr Lys Lys Val Glu Lys Ala Thr Phe Ile Glu Leu 
                325                 330                 335     


Lys Gly Tyr His Leu Ile Lys Lys Gln Leu Lys Asp Arg Asn Val Glu 
            340                 345                 350         


Leu Thr Gln Glu Leu Gln Pro Lys Asp Tyr Asp Ala Ile Ala Ala Ala 
        355                 360                 365             


Ser Thr Phe Phe Lys Asn Asp Ile Glu Phe Arg Asp Tyr Leu Arg Asn 
    370                 375                 380                 


Gln Tyr Leu Asp Ser Asn Gly Lys Arg Lys Ser Asn Ile Ala Asn Lys 
385                 390                 395                 400 


Val Phe Glu Asp Lys Val Ile Ala Ala Val Phe Glu Leu Asn Phe Ser 
                405                 410                 415     


Lys Val Cys His Leu Ser Phe Lys Ser Leu Tyr Lys Leu Ile Pro His 
            420                 425                 430         


Leu Glu Lys Gly Leu Asp Tyr Lys Glu Ala Ile Leu Cys Ala Gly Tyr 
        435                 440                 445             


Asn Phe Arg Asp Glu Asn Lys Arg Asp Lys Lys Leu Phe Leu Pro Val 
    450                 455                 460                 


Ile Gln Gln Glu Thr Asn Pro Val Val His Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Ile Asn Ala Ile Ile Arg Glu Tyr Gly Ser Pro Asn Thr 
                485                 490                 495     


Ile Arg Phe Val Val Ala Asn Asp Leu Ala Lys Thr Tyr Lys Gln Arg 
            500                 505                 510         


Lys Glu Leu Glu Ser Thr Leu Lys Lys Asn Arg Ala Glu Asn Glu Lys 
        515                 520                 525             


Ile Lys Thr Lys Leu Leu Ser Met Gly Ile Ile Asn Pro Thr Gly Met 
    530                 535                 540                 


Asp Ile Val Lys Tyr Lys Leu Trp Tyr Glu Gln Ser Gly Ile Cys Leu 
545                 550                 555                 560 


Tyr Thr Gly Arg Gln Met Glu Leu Ser Ser Leu Phe Lys Pro Gly Tyr 
                565                 570                 575     


Ser Ile Val Asn His Ile Ile Pro Tyr Asn Arg Ser Phe Asp Asp Thr 
            580                 585                 590         


Tyr His Asn Arg Val Leu Thr Leu Thr Glu Thr Lys Tyr Glu Lys Gly 
        595                 600                 605             


Asn Lys Ile Pro Phe Glu Tyr Phe Gly Ala Glu Glu Thr Cys Trp Asn 
    610                 615                 620                 


Glu Tyr Glu Lys Arg Val Ser Thr Ser Lys Lys Ile His Asp Lys Lys 
625                 630                 635                 640 


Arg Glu Lys Leu Leu Ile Arg Asn Phe Asp Ile Glu Tyr Glu Asn Glu 
                645                 650                 655     


Met Ile Ser Asn Asn Val Phe Asp Thr His Tyr Ile Gly Ser Tyr Phe 
            660                 665                 670         


Glu Lys Tyr Ile Asp Glu His Leu Gln Phe Lys Lys Ser Thr Lys Lys 
        675                 680                 685             


Gln Lys Val Phe Ser Ile His Gly Asn Met Val Ala His Leu His Ser 
    690                 695                 700                 


Arg Trp Gly Phe Asn Lys Asn Ser Arg Glu Ile Tyr Leu Asn His Ala 
705                 710                 715                 720 


Val Asp Ala Ala Ile Val Ala Val Leu Thr Asp Gln Leu Ile Arg Asn 
                725                 730                 735     


Ile Ala Asp Tyr Tyr Arg Arg Asp Asn Ile Leu Glu Gln Lys Gly Ser 
            740                 745                 750         


Leu Lys Phe Pro Glu Pro Trp Lys Ser Phe Ser Ile Glu Leu Glu Ala 
        755                 760                 765             


Arg Ile Ser Pro Gln Val Arg Asp Leu Ile Lys Lys Leu Asn Leu Asp 
    770                 775                 780                 


Ser Tyr Leu Asp Val Asp Ile Leu His Ile Lys Pro Val Phe Ile Ser 
785                 790                 795                 800 


Lys Lys Pro Arg Arg Lys Val Asn Gly Glu Ala His Lys Ser Thr Ile 
                805                 810                 815     


Arg Ser Leu Ile Gly Ile Asn Glu Ile Gly Lys Thr Val Thr Cys Ile 
            820                 825                 830         


Lys Thr Arg Leu Glu Asp Ile Pro Phe Asp Glu Asn Gly Asp Phe Pro 
        835                 840                 845             


Met Tyr Gly Lys Asp Thr Asp Lys Tyr Thr Tyr Thr Ala Ile Lys Glu 
    850                 855                 860                 


Arg Tyr Leu Glu Phe Asn Lys Asp Lys Glu Lys Ala Phe Ser Thr Pro 
865                 870                 875                 880 


Leu Tyr Lys Leu Lys Arg Asn Gly Glu Pro Gly Asn Gln Ile Lys Ser 
                885                 890                 895     


Val Lys Ile Ile Asp Thr Arg Asn Ile Val Asn Lys Val Asn Gln Gly 
            900                 905                 910         


Lys Gly Ile Ala Tyr Asn Ser Asn Ile Ile Arg Val Asp Val Phe Ser 
        915                 920                 925             


Arg Asp Gln Lys Tyr Tyr Val Val Pro Ile Tyr Leu Arg Asp Phe Ile 
    930                 935                 940                 


Lys Gly Ser Leu Pro Asn Lys Ile Ile Thr Ala Ala Lys Gly Phe Met 
945                 950                 955                 960 


Gln Trp Pro Glu Thr Asp Asn Ser Tyr Thr Phe Leu Phe Ser Ile Phe 
                965                 970                 975     


Pro Asn Asp Leu Ile Lys Val Val Pro Lys Glu Gly Lys Phe Ile Lys 
            980                 985                 990         


Ala Lys Asn Glu Asn Lys Glu Asn  Ile Asn Leu Thr Glu  Ile Leu Gly 
        995                 1000                 1005             


Tyr Phe  Lys Gly Leu Asp Ser  Ser Thr Gly Ala Phe  Thr Leu Glu 
    1010                 1015                 1020             


Ser His  Asp Gly Ser Leu Leu  Ala Arg Gly Ile Gly  Thr Lys Asn 
    1025                 1030                 1035             


Leu Leu  Leu Ile Glu Lys Tyr  Gln Val Asp Val Leu  Gly Arg Tyr 
    1040                 1045                 1050             


Asn Lys  Val Gly Asn Glu Glu  Arg Gln Asp Ile Lys  Ile Gln Gln 
    1055                 1060                 1065             


Asn 
    


<210>  571
<211>  1070
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  571

Met Ala Thr Arg Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Cys Leu Tyr Asp Gly Asp Ser Ile Arg Asp Ile Gly Val Arg Ile Phe 
            20                  25                  30          


Leu Asp Gly Arg Glu Pro Ser Lys Leu Thr Val Ser Gly Ser Gly Thr 
        35                  40                  45              


Pro Leu Ala Val Lys Arg Arg Asp Ala Arg Gly Ala Arg Arg Arg Arg 
    50                  55                  60                  


Asp Arg Tyr Ile Gly Arg Arg Ser Ala Met Leu Arg Glu Leu Arg Lys 
65                  70                  75                  80  


His Gly Leu Leu Pro Ala Asp Pro Lys Gln Ala Lys Val Leu Glu Thr 
                85                  90                  95      


Gln Asp Pro Tyr Ala Leu Arg Val Arg Ala Leu Asp Glu Lys Leu Glu 
            100                 105                 110         


Pro His Gln Ile Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly 
        115                 120                 125             


Phe Lys Ser Asn Arg Lys Ala Glu Arg Ala Ser Lys Ala Gly Glu Asp 
    130                 135                 140                 


Gly Lys Ile Ala Thr Ala Thr Lys Leu Leu Asp Glu Glu Met Gln Arg 
145                 150                 155                 160 


Leu Gly Ala Arg Thr Leu Gly Glu Phe Leu Val Lys Arg Asp Ser Glu 
                165                 170                 175     


Phe Asp Gly Glu Ala Gly Lys Arg Val Arg Met Arg Ala Asp Ser Lys 
            180                 185                 190         


Ala Tyr Asp Phe Tyr Pro Asp Arg Arg His Val Leu Asp Glu Phe Arg 
        195                 200                 205             


Ala Ile Trp Asn Ala Gln Lys Val Trp His Pro Glu Leu Leu Thr Glu 
    210                 215                 220                 


Gln Ala Trp Asn Asp Ile Phe Arg Val Ile Glu Tyr Gln Arg Glu Leu 
225                 230                 235                 240 


Lys Pro Gln Val Val Gly His Cys Thr Phe Ala Gly Ile Asn Gly Val 
                245                 250                 255     


Pro Ala Asp Glu Lys Arg Leu Pro Lys Ala His Pro Leu Phe Gln Gln 
            260                 265                 270         


Arg Arg Leu Tyr Glu Glu Val Asn Gln Leu Lys Val Val Ala Pro Gly 
        275                 280                 285             


Glu Ala Gly Arg Pro Leu Thr Arg Asp Glu Arg Asp Ala Leu Ile Leu 
    290                 295                 300                 


Lys Leu Gln Asp Lys Lys Ser Val Ser Phe Glu Thr Leu Gly Lys Thr 
305                 310                 315                 320 


Leu Lys Leu Lys Asp Gly Glu Arg Phe Asn Lys Glu Ser Glu Asn Arg 
                325                 330                 335     


Lys Ala Leu Asp Gly Asp Glu Val Arg Ala Ala Leu Ser Gly Lys Lys 
            340                 345                 350         


Leu Phe Gly Thr Arg Trp Phe His Phe Ser Ala Glu Glu Gln Trp Met 
        355                 360                 365             


Ile Ile Glu Arg Leu Leu Glu Glu Glu Asp Ser Glu Thr Leu Leu Thr 
    370                 375                 380                 


Trp Leu Lys Ile Gly Tyr Arg Leu Asp Asp Glu Ala Ala Leu Ala Val 
385                 390                 395                 400 


Ala Asn Ala Lys Leu Pro Glu Gly His Gly Arg Phe Gly Leu Ser Ala 
                405                 410                 415     


Thr Thr Ala Leu Leu Lys Glu Leu Lys Ala Asp Val Cys Thr Tyr Ala 
            420                 425                 430         


Glu Ala Ala Lys Arg Ala Gly Phe His His Ser Asp Phe Arg Thr Gly 
        435                 440                 445             


Glu Val Phe Asp Gln Leu Pro Tyr Tyr Gly Glu Ile Leu Ala Arg Glu 
    450                 455                 460                 


Ile Ala Pro Gly Lys Pro Glu Tyr Gly Asp Glu Asn Glu Arg Arg Phe 
465                 470                 475                 480 


Gly Lys Ile Thr Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu Thr 
                485                 490                 495     


Lys Leu Val Asn Ala Ile Ile Arg Leu His Gly Lys Pro Asp Tyr Ile 
            500                 505                 510         


Phe Val Glu Leu Ala Arg Glu Leu Lys Leu Asn Glu Lys Glu Lys Ile 
        515                 520                 525             


Ala Tyr Asn Arg Gln Leu Lys Glu Asn Thr Asp Ala Ala Arg Asp Arg 
    530                 535                 540                 


Gly Ala Lys Leu Gly Thr Met Gly Val Arg Asp Thr Gly Arg Asn Arg 
545                 550                 555                 560 


Leu Ile Leu Arg Leu Trp Thr Glu Leu Asn Pro Gly Asp Ala Leu Asn 
                565                 570                 575     


Arg Arg Cys Pro Phe Cys Gly Glu Gln Ile Ser Gln Arg Met Ile Gln 
            580                 585                 590         


Asn Gly Glu Val Glu Ile Glu His Ile Ile Pro Tyr Ser Gln Cys Phe 
        595                 600                 605             


Asp Asp Gly Ile Gly Asn Lys Val Leu Ser His Val Arg Cys Asn Arg 
    610                 615                 620                 


Gln Lys Gly Asp Gln Thr Pro Trp Glu Arg Trp Gly His Asp Pro Asp 
625                 630                 635                 640 


Arg Trp Ser Ile Ile Gln Glu Gln Val Ala His Leu His Arg Ser Lys 
                645                 650                 655     


Gln Trp Arg Phe Gly Pro Asp Ala Arg Asp Arg Tyr Ala Ala Glu Glu 
            660                 665                 670         


Asp Ser Phe Leu Pro Arg Gln Leu Ile Asp Thr Gln Tyr Leu Thr Arg 
        675                 680                 685             


Ile Ala Ala Lys Tyr Leu Ala Cys Leu Tyr Pro Glu Arg Gly Glu Arg 
    690                 695                 700                 


His Val Phe Ala Val His Gly Gly Met Thr Ala Met Leu Arg Arg Leu 
705                 710                 715                 720 


Trp Asp Leu Asn Ser Ile Leu Leu Asp His Asn Trp Val Glu Asn Leu 
                725                 730                 735     


Asn Ser Asn Ala Pro Lys Asn Arg Leu Asp His Arg His His Ala Ile 
            740                 745                 750         


Asp Ala Ala Val Val Gly Ile Met Thr Pro Gly Leu Ile Ala Glu Ile 
        755                 760                 765             


Ala Lys Ala Ala Ala Arg Ala Glu Lys Gln Asn Leu Asp Arg Leu Phe 
    770                 775                 780                 


Glu Glu Leu Pro Met Pro Trp Ala Gln His Pro Asn Asp Arg Arg Phe 
785                 790                 795                 800 


Arg Asp Glu Leu Arg Glu Lys Ile Leu Ala Ala Thr Val Ser His Lys 
                805                 810                 815     


Ala Asp His Gly Arg Gln Gly Lys Pro Glu Arg Gly Arg Asp Ile Thr 
            820                 825                 830         


Ala Gly Lys Leu His Asn Asp Thr Ala Tyr Gly Phe Thr Gly Leu Thr 
        835                 840                 845             


Ser Asp Thr Gly Leu Pro Val Val Val His Arg Val Pro Leu Thr Ser 
    850                 855                 860                 


Leu Thr Pro Lys Asp Ile Ala Asp Pro Asp Arg Ile Pro Asp Thr Thr 
865                 870                 875                 880 


Leu Arg Gly Ser Leu Trp Gln Ala Thr Arg Asp Leu Ser Gly Lys Glu 
                885                 890                 895     


Phe Glu Lys Ala Leu Leu Asp Phe Ser Arg Thr His Ala Gln Phe Lys 
            900                 905                 910         


Gly Ile Arg Arg Val Arg Val Arg Glu Gly Leu Thr Leu Ile Pro Ile 
        915                 920                 925             


Arg Asn His Ala Gly Lys Pro Tyr Lys Gly Tyr Leu Gly Asp Ser Asn 
    930                 935                 940                 


Ala Arg Tyr Asn Val Trp Gln Met Pro Asp Gly Arg Trp Val His Gln 
945                 950                 955                 960 


Val Ile Ser Thr Phe Asp Leu His Gln Pro Gly Phe Ile Glu Gln Arg 
                965                 970                 975     


Pro His Pro Ala Ala Lys Lys Val Leu Ser Leu Arg Gln Asn Asp Leu 
            980                 985                 990         


Ile Ala Ile Glu Arg Asp Gly Ala  Leu Arg Gln Ile Met  Arg Val Gln 
        995                 1000                 1005             


Lys Tyr  Gly Gln Asn Gly Gln  Ile Phe Leu Ile Glu  His Asn Glu 
    1010                 1015                 1020             


Ala Gly  Asn Met Ala Glu Arg  Asn Lys Asn Thr Asp  Asp Pro Phe 
    1025                 1030                 1035             


Lys Phe  Tyr Gly Pro Gly Ala  Ser Ala Leu Arg Asn  Met Lys Ala 
    1040                 1045                 1050             


Arg Gln  Val Arg Ile Asp Glu  Leu Gly Arg Ile Phe  Asp Pro Gly 
    1055                 1060                 1065             


Pro Arg  
    1070 


<210>  572
<211>  1070
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  572

Met Ala Lys Asp Phe Glu Tyr Ile Leu Gly Leu Asp Leu Gly Glu Thr 
1               5                   10                  15      


Ser Ile Gly Trp Ser Val Val Arg Leu Asn Glu Gly Val Pro Glu His 
            20                  25                  30          


Leu Glu Arg Leu Gly Val Arg Ile Cys Ser Asp Gly Arg Asp Ala Gln 
        35                  40                  45              


Ser His Glu Pro Leu Ser Val Ala Arg Arg Ile Ala Arg Gly Leu Arg 
    50                  55                  60                  


Arg Arg Arg Asp Arg Tyr Ile His Arg Arg Ile Arg Leu Thr Asp Val 
65                  70                  75                  80  


Leu Val Lys Tyr Gly Leu Met Pro Glu Asp Ile Lys Glu Arg Lys Lys 
                85                  90                  95      


Leu Gln Ala Leu Asn Pro Tyr Arg Leu Arg Ser Glu Ala Leu Asp Arg 
            100                 105                 110         


Lys Leu Glu Pro Tyr Glu Leu Gly Arg Val Leu Phe His Ile Asn Gln 
        115                 120                 125             


Arg Arg Gly Phe Lys Ser Asn Met Lys Thr Asp Arg Lys Asp Ser Glu 
    130                 135                 140                 


Ser Gly Ala Met Lys Gln Ala Ile Leu Lys Thr Arg Ala Lys Met Glu 
145                 150                 155                 160 


Ala Ser Gly Ala Arg Thr Leu Gly Glu Phe Leu Tyr Phe Leu Asn Lys 
                165                 170                 175     


Asp Lys Thr Ser Thr Gln Glu Tyr Val Pro Val Arg Met Arg Ser His 
            180                 185                 190         


Ser Glu Lys Asn Lys Thr Val Tyr Glu Met Tyr Pro Asp Arg Ala Met 
        195                 200                 205             


Tyr Glu Asp Glu Val His Lys Ile Phe Glu Gln Gln Glu Leu Ser Pro 
    210                 215                 220                 


Glu Ala Gln Thr Glu Ile Phe Asn Thr Ile Phe Phe Gln Arg Pro Leu 
225                 230                 235                 240 


Lys Pro Val Glu Leu Gly Phe Cys Glu Phe Glu Asp Gly Glu Arg Arg 
                245                 250                 255     


Ala Tyr Thr Ala Ser Val Val Phe Gln Lys Phe Arg Ala Leu Gln Gln 
            260                 265                 270         


Ile Asn Gln Ile Tyr Val Gln Asp Asp Tyr Glu Arg Arg Pro Leu Thr 
        275                 280                 285             


Lys Glu Glu Lys Asp Ile Leu Lys His Phe Leu Leu Glu Asp Phe Ser 
    290                 295                 300                 


Ala Leu Asp Lys Asn Gly Leu Leu Thr Trp Ala Lys Val Arg Thr Leu 
305                 310                 315                 320 


Leu Lys Leu Gln Lys Lys Lys Phe Asn Leu Glu Ser Glu Arg Arg Lys 
                325                 330                 335     


Gly Leu Asn Ala Asp Val Thr Ser Trp Lys Leu Ser Asn Ala Ala Cys 
            340                 345                 350         


Phe Gly Arg Glu Trp Phe Ser Phe Ser Asp Ala Lys Lys Glu Glu Ile 
        355                 360                 365             


Val Ala Leu Ile Thr Gly Ala Glu Lys Glu Glu Glu Leu Ile Ala Glu 
    370                 375                 380                 


Leu Lys Thr Lys Tyr Gly Leu Ser Asp Glu Arg Ala Lys Asn Thr Ala 
385                 390                 395                 400 


Asn Val Ser Leu Pro Asp Gly Val Ala Ser Leu Ser Ala Lys Ala Met 
                405                 410                 415     


Lys Lys Leu Ile Pro Phe Leu Glu Glu Gly Met Leu Tyr Asp Asp Ala 
            420                 425                 430         


Val Lys Gln Ala Gly Tyr Val Phe Ser Ser Ser Phe Lys Gly Val Leu 
        435                 440                 445             


Pro Glu Lys Tyr Glu Thr Phe Ile Glu Pro Glu Ser Gly Glu Cys Phe 
    450                 455                 460                 


Asp Glu Leu Pro Tyr Tyr Gly Gln Val Leu Arg Arg Tyr Val Leu Gly 
465                 470                 475                 480 


Gly Val Pro Ser Glu Glu Asn Lys Leu Phe Pro Glu Lys Tyr Tyr Gly 
                485                 490                 495     


Lys Ile Asn Asn Pro Thr Val His Ile Val Leu Asn Gln Leu Arg Lys 
            500                 505                 510         


Leu Ile Asn Ser Leu Val Ala Lys Tyr Gly His Pro Arg Lys Ile Val 
        515                 520                 525             


Val Glu Met Ala Arg Asp Leu Lys Leu Ser Lys Lys Gln Lys Asp Ala 
    530                 535                 540                 


Ile Ala Lys Glu Gln Ala Leu Asn Glu Arg Glu Asn Lys Arg Ile Gly 
545                 550                 555                 560 


Ala Glu Leu Glu Arg Leu Asp Ile Arg Asn Asn Tyr Asp Asn Arg Met 
                565                 570                 575     


Lys Tyr Lys Leu Trp Glu Asp Leu Ser Thr Asp Pro Thr Lys Arg Ser 
            580                 585                 590         


Cys Pro Phe Cys Gly Lys Gln Ile Ser Gln Gln Asp Ile Phe Ser Pro 
        595                 600                 605             


Glu Phe Glu Ile Glu His Leu Ile Pro Phe Ser Arg Ser Phe Met Asp 
    610                 615                 620                 


Gly Arg Ser Asn Lys Val Leu Ser Cys Arg Arg Cys Asn Arg Glu Lys 
625                 630                 635                 640 


Gly Asn Lys Thr Pro Phe Glu Ala Phe Gly Asn Asp Ser Thr Arg Trp 
                645                 650                 655     


Asn Ala Ile Met Gly Arg Val Glu Ser Leu Pro Glu Asn Lys Arg Trp 
            660                 665                 670         


Lys Phe Ala Ser Asp Ala Met Ser Arg Phe Glu Arg Asp Gly Asp Leu 
        675                 680                 685             


Leu Ala Arg Met Met Thr Asp Thr Gln Tyr Met Ala Arg Ile Ala Arg 
    690                 695                 700                 


Arg Tyr Leu Cys Cys Ala Ala Asn Pro Asn Gln Val Tyr Gly Ile Pro 
705                 710                 715                 720 


Gly Gln Leu Thr Ala Lys Leu Arg Glu His Trp Gly Leu Asn Ser Ile 
                725                 730                 735     


Leu Ser Asp Gln Lys Glu Lys Asp Arg Ser Asp His Arg His His Ala 
            740                 745                 750         


Ile Asp Ala Phe Val Val Ala Cys Thr Asp Arg Gly Thr Leu Gln Lys 
        755                 760                 765             


Tyr Ser Thr Glu Arg Arg Asp Tyr Ala Lys Pro Glu Met Pro Ser Glu 
    770                 775                 780                 


Lys Ala Pro His Ile Pro Phe Ala Asp Phe Arg Leu Ser Asp Ile Lys 
785                 790                 795                 800 


Arg Val Phe Asp Glu Ile Ile Val Ser His Lys Pro Asp Arg Gly Asn 
                805                 810                 815     


Ala Arg Ala Ala Ile Leu Lys Gly Gly Thr Val Ala Lys Leu His Glu 
            820                 825                 830         


Glu Thr Tyr Tyr Gly Met Gly Gly Glu Gly Ser Lys Lys Gly Thr Trp 
        835                 840                 845             


Lys Leu Ile Gln Arg Ile Pro Val Ser Glu Ile Lys Lys Ala Ser Asp 
    850                 855                 860                 


Leu Asp Cys Leu Ile Asp Gln Lys Gly Thr Ala Asn Pro Leu Arg Val 
865                 870                 875                 880 


Leu Leu Val Gly Ala Ser Glu Ser Glu Gln Glu Gln Ile Ile Ala Ser 
                885                 890                 895     


Tyr Phe Ala Arg Arg Gly Thr His Lys Val Arg Thr Tyr Arg Glu Arg 
            900                 905                 910         


Asn Lys Asp Val Ile Leu Pro Phe Lys Asp Lys Asp Gly Lys Leu Tyr 
        915                 920                 925             


Arg Tyr Ala Val Tyr Gly Ser Asn Ala Tyr Ala Glu Ile Tyr Cys Pro 
    930                 935                 940                 


Asp Arg Gly Lys Asn Ala Gly Lys Trp Gln Val Glu Ile Ile Pro Asn 
945                 950                 955                 960 


Tyr Cys Ala His Gln Lys Gly Phe Ile Pro Lys Trp Arg His Thr Asp 
                965                 970                 975     


Ala His Ala Lys Leu Ile Met Arg Leu Tyr Arg Asn Asp Met Val Ala 
            980                 985                 990         


Tyr Glu Lys Glu Gly Lys Thr Val  Ile Ala Arg Val Lys  Lys Met Gly 
        995                 1000                 1005             


Gly Asn  Gly Val Ile Val Phe  Arg Asn His Leu Ile  Ala Lys Glu 
    1010                 1015                 1020             


Glu Gly  Asp Arg Leu Ser Trp  Gln Ala Val Val Ser  Gly Met Gln 
    1025                 1030                 1035             


Ser Ala  Asn Leu Arg Lys Ile  Tyr Val Asp Val Leu  Gly Arg Val 
    1040                 1045                 1050             


Ile Asp  Pro Lys Arg Pro Met  Thr Gln Asn Glu Thr  Asp Gly Gln 
    1055                 1060                 1065             


Asp Asn  
    1070 


<210>  573
<211>  1070
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  573

Met Ser His Phe Arg Leu Lys Gln Leu Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Leu Gly Ile Ala Ser Val Gly Trp Ala Val Val Glu Ile Asp Thr Glu 
            20                  25                  30          


Glu Asn Pro Thr Arg Leu Ile Asp Val Gly Val Arg Thr Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Ala Ala Arg Arg Gln 
    50                  55                  60                  


Ala Arg Ser Ala Arg Arg Leu Ile Arg Arg Arg Thr His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Ile Leu His Ala Asp Asp 
                85                  90                  95      


Leu Asn Ala Glu Gly Leu Val Lys Glu Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Val Asp Gly Leu Asp Arg Lys Leu Met Pro Lys Glu Trp Ala 
        115                 120                 125             


Ala Val Leu Leu His Leu Val Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Ser Glu Gly Ala Asp Lys Glu Leu Gly Arg Leu Leu Ala 
145                 150                 155                 160 


Gly Val His Glu Asn His Gln Ala Leu Gln Ser Ser Glu Tyr Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Val Lys Lys Phe Gln Ala Gln Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Thr His Thr Phe Asp Arg Lys Asp Leu 
        195                 200                 205             


Gln Gln Glu Leu His Gln Leu Phe Ala Ala Gln Gln Lys Tyr Gly Asn 
    210                 215                 220                 


Pro Phe Val Ser Asp Gly Leu Lys Gln Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Gln Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


Arg Cys Thr Phe Glu Thr Glu Glu Phe Lys Ala Ala Lys His Thr Tyr 
            260                 265                 270         


Ser Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Gln Glu Asn Gly Asn Glu Arg Cys Leu Thr Ala Gln Glu Arg Gly Gln 
    290                 295                 300                 


Ile Ile Asp Leu Pro Tyr Lys Lys Ala Lys Leu Thr Tyr Ala Gln Val 
305                 310                 315                 320 


Arg Lys Glu Leu Ser Leu Pro Glu Asn Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Glu Asn Ala Glu Thr Ala Ala Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Arg Lys Ala Leu Glu Lys Ala Gly Leu Lys Thr Glu 
        355                 360                 365             


Trp Gln Gly Leu Ala Cys Lys Pro Glu Leu Leu Asp Ala Ile Gly Thr 
    370                 375                 380                 


Val Phe Ser Leu Tyr Lys Thr Asp Glu Asp Ile Ser Thr Arg Leu Gln 
385                 390                 395                 400 


Gly Gln Leu Ser Glu Asp Val Ile Asn Ala Leu Leu Ala Gly Leu Asn 
                405                 410                 415     


Phe Asp Lys Phe Ile Gln Leu Ser Leu Lys Ala Leu Ser Lys Ile Leu 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Gln Arg Tyr Asp Glu Ala His Ala Ala Leu 
        435                 440                 445             


Tyr Val Arg Ser Thr Pro Gln Pro Gln Arg Leu Leu Pro Gln Ile Thr 
    450                 455                 460                 


Ala Asp Glu Ile Cys Asn Pro Val Val Leu Arg Thr Leu Ser Gln Ala 
465                 470                 475                 480 


Arg Lys Val Ile Asn Ala Ile Val Arg Arg Tyr Gly Ser Pro Ala Arg 
                485                 490                 495     


Ile His Ile Glu Thr Gly Arg Glu Val Gly Lys Ser Phe Lys Asp Arg 
            500                 505                 510         


Gln Glu Ile Gln Lys Arg Gln Glu Asp Asn His Lys Glu Arg Lys Gln 
        515                 520                 525             


Ala Ala Glu Lys Phe Leu Glu Tyr Phe Pro Asn Phe Val Gly Gln Pro 
    530                 535                 540                 


Lys Ala Lys Asp Ile Leu Lys Leu Arg Leu Tyr Gln His Gln His Gly 
545                 550                 555                 560 


Gln Cys Leu Tyr Ser Gly Lys Thr Leu Asp Ile Cys Arg Leu Asn Glu 
                565                 570                 575     


Lys Gly Tyr Ala Glu Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp 
            580                 585                 590         


Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Ala Gly Glu Asn Gln 
        595                 600                 605             


Asn Lys Gly Asn Gln Thr Pro Tyr Glu Trp Leu Gly Gly Ala Ser Asn 
    610                 615                 620                 


Ser Pro Arg Trp Gln Glu Phe Val Ala Arg Val Gly Ser Ser Arg Phe 
625                 630                 635                 640 


His His Ser Lys Lys Gln Arg Ile Leu Leu Arg Gln Leu Asp Glu Glu 
                645                 650                 655     


Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe 
            660                 665                 670         


Leu Cys Lys Phe Ile Glu Glu Asn Met Leu Leu Leu Gly Arg Gly Lys 
        675                 680                 685             


Arg Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Ser Phe Leu Arg Ser 
    690                 695                 700                 


Arg Trp Gly Leu Arg Lys Val Arg Glu Glu Asn Asp Arg His His Ala 
705                 710                 715                 720 


Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala Ile Gln Gln Arg 
                725                 730                 735     


Ile Thr Tyr Phe Val Arg Gln Arg Glu Met Asn Ile Phe Asp Gly Glu 
            740                 745                 750         


Leu Ile Asp Lys Glu Thr Ser Glu Val Ser Arg Pro Tyr Phe Pro Gln 
        755                 760                 765             


Pro Trp Asp Phe Phe Arg Gln Glu Val Met Met Arg Val Phe Gly Lys 
    770                 775                 780                 


Pro Asp Gly Asp Pro Ser Phe Pro Glu Ala Asp Thr Lys Glu Thr Leu 
785                 790                 795                 800 


Cys Thr Leu Leu Glu Thr Arg Leu Ser Ser Arg Pro Gln Ala Val His 
                805                 810                 815     


Glu Tyr Val Thr Pro Leu Phe Val Ser Arg Ala Pro Thr Arg Lys Met 
            820                 825                 830         


Ser Gly Gln Gly His Lys Glu Thr Val Lys Ser Ala Lys Leu Leu His 
        835                 840                 845             


Glu Gly Lys Ser Val Leu Arg Val Pro Leu Thr Gln Leu Lys Pro Asn 
    850                 855                 860                 


Leu Leu Glu Lys Met Val Asn Arg Asp Arg Glu Pro Gln Leu Tyr Glu 
865                 870                 875                 880 


Ala Leu Lys Glu Arg Leu Ala Ala Tyr Gly Asn Asp Pro Ala Lys Ala 
                885                 890                 895     


Phe Ala Thr Glu Phe Arg Lys Lys Gly Gly Gln Leu Val Lys Ala Val 
            900                 905                 910         


Arg Val Glu Asp Thr Gln Lys Ser Gly Val Leu Leu His Lys Gly Thr 
        915                 920                 925             


Gly Val Ala Asp Asn Ala Thr Met Val Arg Val Asp Val Phe Glu Lys 
    930                 935                 940                 


Ser Gly Lys Tyr Tyr Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Gln 
945                 950                 955                 960 


Gly Ile Leu Pro Asn Lys Ala Val Ile Gln Gly Lys Asp Glu Lys Asn 
                965                 970                 975     


Trp Asp Glu Met Asp Glu Thr Tyr Thr Phe Lys Phe Ser Met Tyr Pro 
            980                 985                 990         


Asn Asp Leu Val Glu Ile Asn Thr  Gly Lys Gln Arg Leu  Phe Gly Tyr 
        995                 1000                 1005             


Tyr Ile  Gly Leu Asp Arg Ala  Thr Gly Ala Ile Ser  Ile Arg Glu 
    1010                 1015                 1020             


His Asp  Leu Asp Asn Phe Lys  Gly Lys Asn Gly Ile  Tyr Arg Val 
    1025                 1030                 1035             


Gly Val  Lys Thr Ala Ile Ser  Phe Lys Lys Tyr Gln  Thr Asp Glu 
    1040                 1045                 1050             


Leu Gly  Lys Val Val Val Pro  Cys Arg Pro Lys Lys  Arg Leu Pro 
    1055                 1060                 1065             


Val Arg  
    1070 


<210>  574
<211>  1071
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  574

Met Arg Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly Trp Trp Leu 
1               5                   10                  15      


Tyr Gln Thr Asp Asp Gly Thr Ile Thr Asp Val Leu Asp Gly Gly Val 
            20                  25                  30          


Arg Ile Phe Ser Asp Gly Arg Asp Pro Gln Ser Lys Glu Ser Leu Ala 
        35                  40                  45              


Val Asp Arg Arg Val Ala Arg Ala Gln Arg Arg Arg Arg Asp Arg Tyr 
    50                  55                  60                  


Leu Arg Arg Lys Ala Ala Leu Met Lys Arg Met Ala Gly Ala Gly Leu 
65                  70                  75                  80  


Met Pro Thr Asp Gln Ala His Ala Lys Lys Leu Glu Ser Leu Asp Pro 
                85                  90                  95      


Tyr Ala Leu Arg Ala Glu Gly Leu Asp Cys Glu Leu Pro Leu Thr His 
            100                 105                 110         


Leu Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe Lys Ser 
        115                 120                 125             


Asn Arg Lys Thr Asp Arg Gly Asp Asn Glu Ser Gly Lys Ile Lys Asp 
    130                 135                 140                 


Ala Thr Ala Arg Leu Asp Trp Ala Met Arg Asp Ala Lys Ala Arg Thr 
145                 150                 155                 160 


Tyr Gly Glu Phe Leu His Thr Arg Gln Gln Lys Ala Asp Asp Pro Lys 
                165                 170                 175     


Arg Met Pro Thr Val Arg Thr Arg Leu Thr Val Ala Arg Arg Asp Asn 
            180                 185                 190         


Ala Glu Lys Glu Glu Ala Gly Tyr Asp Phe Tyr Pro Asp Arg Arg His 
        195                 200                 205             


Leu Ser Glu Glu Phe Asp Ala Leu Trp Ala Ala Gln Ala Asp Tyr His 
    210                 215                 220                 


Glu Ala Leu Ser Asp Asp Leu Arg Asp Gln Ile Lys Thr Ile Ile Phe 
225                 230                 235                 240 


His Gln Arg Pro Leu Lys Thr Pro Glu Val Gly Leu Cys Leu Phe Thr 
                245                 250                 255     


Asp Glu Arg Arg Ile Pro Ser Ala His Pro Leu Asn Gln Arg Arg Ile 
            260                 265                 270         


Leu Leu Glu Thr Val Asn Ala Leu Arg Ile Val Ala Arg Gly Glu Ala 
        275                 280                 285             


Ala Arg Arg Leu Thr Leu Glu Glu Arg Asp Gln Ile Val His Ala Leu 
    290                 295                 300                 


Asp Asn Lys Asn His Thr Lys Thr Leu Ser Gly Met Ala Met Lys Leu 
305                 310                 315                 320 


Lys Ala Leu Gly Lys Val Ile Arg Leu Arg Ser Asp Gln Thr Phe Thr 
                325                 330                 335     


Leu Glu Thr Ala Asn Arg Asp Ala Ile Ala Cys Asp Leu Val Arg Ala 
            340                 345                 350         


Ser Leu Ser His Pro Asp Arg Met Gly Gly Val Trp Thr Thr Leu Asp 
        355                 360                 365             


Glu Gly Ala Gln Trp Glu Val Val Gln Arg Ile Arg Ala Val Gln Ser 
    370                 375                 380                 


Asp Ser Glu Phe Asp Ala Leu Val Val Trp Leu Glu Gln Asn His Gly 
385                 390                 395                 400 


Leu Asp Asn Ala His Ala Gln Ala Thr Ala Asn Ala Pro Leu Pro Glu 
                405                 410                 415     


Gly Tyr Gly Arg Met Gly Leu Met Ala Thr Arg Arg Ile Leu Ala Ala 
            420                 425                 430         


Leu Gln Ser Asp Val Met Pro Tyr Ser Asp Ala Val Ala Ala Cys Gly 
        435                 440                 445             


Trp Ser His Ser Gly Gly Pro Thr Gly Glu Val Leu Glu Lys Leu Pro 
    450                 455                 460                 


Tyr Tyr Gly Lys Ile Leu Asp Arg His Val Ile Pro Gly Thr Tyr Asp 
465                 470                 475                 480 


Glu Asn Asn Glu Asp Val Lys Arg Tyr Gly Arg Ile Thr Asn Pro Thr 
                485                 490                 495     


Val His Ile Gly Leu Asn Gln Ile Arg Arg Leu Val Asn Arg Ile Ile 
            500                 505                 510         


Cys Ile His Gly Lys Pro Asp Glu Ile Val Val Glu Leu Ala Arg Asp 
        515                 520                 525             


Leu Lys Asn Ser Glu Asp Gln Lys Arg Glu Ile Gln Lys Ile Ile Lys 
    530                 535                 540                 


Lys Asn Thr Asp Ala Ala Ile Lys Arg Gly Glu Lys Leu Ile Asn Glu 
545                 550                 555                 560 


Leu Gly Gln Lys Asp Thr Gly Ala Asn Arg Leu Val Leu Arg Leu Trp 
                565                 570                 575     


Glu Asp Leu Gly His Asp Val Met Thr Arg Asn Cys Pro Tyr Ser Gly 
            580                 585                 590         


Lys Arg Ile Ser Ala Gly Met Leu Phe Asp Gly Ser Cys Asp Val Asp 
        595                 600                 605             


His Ile Leu Pro Tyr Ser Arg Thr Leu Asp Asp Gly Ile Ala Asn Lys 
    610                 615                 620                 


Thr Leu Cys Leu Lys Glu Ala Asn Arg Gln Lys Ser Asn Asn Thr Pro 
625                 630                 635                 640 


Trp Glu Val Trp Gly Glu Thr Glu Gln Trp Asp Thr Ile Ala Ala Asn 
                645                 650                 655     


Leu Lys Asn Leu Pro Asp Asn Lys Ala Trp Arg Phe Ala Ser Asp Ala 
            660                 665                 670         


Met Gln Arg Phe Glu Gly Glu Asn Asp Phe Ser Ala Arg Ala Leu Lys 
        675                 680                 685             


Asp Thr Gln Tyr Leu Ser Arg Ile Ala Arg Ala Tyr Leu Asp Ala Leu 
    690                 695                 700                 


Tyr Asp Gly Gly Asp Gly Asn Ser His Val Trp Val Val Pro Gly Arg 
705                 710                 715                 720 


Leu Thr Glu Met Leu Arg Arg His Trp Gly Leu Asn Gly Leu Glu Ala 
                725                 730                 735     


Leu Thr Asp Ser Asp Glu Asn Thr Val Lys Ala Lys Asn Arg Met Asp 
            740                 745                 750         


His Arg His His Ala Ile Asp Ala Ala Val Val Ala Ala Thr Asp Arg 
        755                 760                 765             


Gly Leu Ile Gln Arg Ile Ser Lys Ile Ala Lys Arg Asp Glu Gln Ala 
    770                 775                 780                 


Gly Ala Glu Glu Val Ala Arg Ser Val Ser Pro Pro Trp Asp Gly Phe 
785                 790                 795                 800 


Arg Asp Asp Ile Ala Ala Gln Leu Ser Arg Ile Ile Val Ser His Arg 
                805                 810                 815     


Ala Asp His Gly Arg Ile Asp Ser Gln Ala Lys Gln Gln Gly Glu Asp 
            820                 825                 830         


Ala Thr Ala Gly Gln Leu His Met Asp Thr Ala Tyr Gly Val Val Asp 
        835                 840                 845             


Ala Gly Arg Val Val Ser Arg Lys Pro Leu Leu Ser Leu Ser Glu Ser 
    850                 855                 860                 


Asp Ile Lys Lys Ile Arg Asp Pro Asp Leu Gln Arg His Leu Thr Arg 
865                 870                 875                 880 


Val Thr Lys Gly Leu Asp Lys Lys Glu Phe Glu His Ala Leu Ser Asp 
                885                 890                 895     


Phe Ala Asn Ala Arg Lys Leu Pro Asp Gln Ser Glu Asn Pro Tyr Phe 
            900                 905                 910         


Gly Met Arg Arg Val Arg Leu Ile Asp Ala Leu Gln Asp Ser Ala Arg 
        915                 920                 925             


Val Ser Val Arg Asp Arg Asp Gly His Ile Tyr Lys Ala Tyr Lys Ala 
    930                 935                 940                 


Gly Ser Asn His Cys Tyr Glu Val Trp Arg Leu Pro Asp Gly Lys Ile 
945                 950                 955                 960 


Lys Pro Trp Ala Ile Ser Thr Phe Glu Ala His Gln Thr Gly Thr Glu 
                965                 970                 975     


Ser Lys Pro His Pro Ala Ala Lys Arg Leu Leu Arg Val Phe Lys Arg 
            980                 985                 990         


Asp Met Val Ala Ile Glu Arg Asp  Gly Glu Thr Leu Met  Cys Tyr Val 
        995                 1000                 1005             


Gln Lys  Met Asp Ile Ala Asn  Gly Leu Phe Leu Val  Pro His Thr 
    1010                 1015                 1020             


Glu Gly  Asn Ala Asp Ala Arg  Asn Arg Asp Lys Asp  Asp Ser Phe 
    1025                 1030                 1035             


Lys Phe  Ile Gln Met Ser Ala  Val Ser Leu Ile Arg  Ala Lys Ile 
    1040                 1045                 1050             


Arg Arg  Ile His Val Asp Glu  Met Gly Arg Leu Arg  Asp Gln Gly 
    1055                 1060                 1065             


Pro Pro  Arg 
    1070     


<210>  575
<211>  1071
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  575

Met Arg Tyr Thr Ile Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Glu Asp Lys Ser Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Val Phe Lys Lys Ala Glu Glu Pro Asp Gly Lys Ser Leu Asn Leu Ala 
        35                  40                  45              


Arg Arg Glu Ala Arg Gly Ser Arg Arg Arg Ile Arg Arg Arg Ala Thr 
    50                  55                  60                  


Arg Met Gln Lys Val Lys Glu Leu Leu Val Lys Tyr Asn Leu Val Ser 
65                  70                  75                  80  


Glu Lys Glu Leu Glu Thr Leu Tyr Ile Ile Thr Glu Thr Thr Pro Asp 
                85                  90                  95      


Val Tyr Glu Leu Arg Ala Lys Gly Leu Asp Arg Leu Leu Ser Arg Gln 
            100                 105                 110         


Glu Phe Ala Arg Val Leu Thr Asn Ile Ala Lys Arg Arg Gly Tyr Lys 
        115                 120                 125             


Ser Asn Arg Lys Ile Asp Glu Glu Asp Lys Glu Val Gly Lys Leu Ser 
    130                 135                 140                 


Lys Gly Thr Ile Glu Asn Lys Arg Ile Leu Gln Glu Lys Gly Tyr Arg 
145                 150                 155                 160 


Thr Ile Gly Glu Met Phe Tyr Lys Asp Glu Lys Phe Glu Tyr Asn Lys 
                165                 170                 175     


Arg Asn Lys Ala Gly Glu Tyr Asn Ser Thr Val Leu Arg Ser Met Leu 
            180                 185                 190         


Ile Asp Glu Val His Thr Leu Phe Glu Met Gln Arg Lys Phe Lys Ser 
        195                 200                 205             


Glu Phe Ala Asn Glu Glu Phe Glu Lys Glu Tyr Val Glu Val Met Thr 
    210                 215                 220                 


Tyr Gln Lys Pro Tyr Ile Thr Pro Glu Leu Leu Glu Lys Met Leu Gly 
225                 230                 235                 240 


Lys Cys Thr Phe Glu Lys Gly Glu Pro Arg Ala Pro Lys Asn Ser Tyr 
                245                 250                 255     


Thr Phe Glu Arg Phe Met Leu Leu Gln Lys Val Asn Asn Leu Asn Ile 
            260                 265                 270         


Tyr Ile Asn Gly Glu Lys Val Glu Ile Pro Gln Glu Lys Lys Lys Glu 
        275                 280                 285             


Ile Ile Glu Leu Ala Tyr Lys Gln Ser Glu Ile Lys Tyr Thr Gln Leu 
    290                 295                 300                 


Arg Lys Lys Leu Glu Leu Pro Ile Glu Ala Arg Phe Ala Asp Leu Asn 
305                 310                 315                 320 


Tyr Gln Ile Lys Arg Thr Glu Asp Glu Ser Glu Glu Ala Leu Val Lys 
                325                 330                 335     


Lys Ile Glu Asp Lys Lys Phe Val Lys Leu Glu Gly Trp His Lys Ile 
            340                 345                 350         


Arg Leu Ala Leu Lys Glu Asn Lys Asp Lys Phe Glu Lys Ile Glu Lys 
        355                 360                 365             


Asn Pro Glu Leu Gln Asn Ile Ile Ala Asp Ala Leu Val Arg Asn Lys 
    370                 375                 380                 


Thr Asp Glu Thr Ile Lys Asn Tyr Leu Glu Lys Ser Asp Ile Asp Glu 
385                 390                 395                 400 


Glu Ile Ile Asn Ala Ile Leu Asn Ile Asn Phe Thr Lys Phe Gly His 
                405                 410                 415     


Leu Ser Tyr Lys Ala Met Asn Lys Ile Ile Pro Glu Leu Glu Lys Gly 
            420                 425                 430         


Leu Thr Tyr Asp Lys Ala Cys Glu Asn Val Gly Tyr Glu Phe Lys Gly 
        435                 440                 445             


Arg Lys Asn Glu Leu Gln Tyr Lys Leu Pro Pro Val Thr Glu Leu Glu 
    450                 455                 460                 


Asp Glu Ile Leu Asn Ser Val Val Leu Arg Ala Val Ser Gln Thr Arg 
465                 470                 475                 480 


Lys Val Ile Asn Ala Ile Ile Asp Lys Tyr Gly Ser Pro Thr Ala Ile 
                485                 490                 495     


Tyr Ile Glu Thr Ala Arg Glu Leu Ser Lys Pro Tyr Asp Glu Arg Met 
            500                 505                 510         


Lys Ile Glu Lys Arg Gln Lys Glu Asn Ala Ala Asn Asn Asp Gln Ile 
        515                 520                 525             


Lys Glu Tyr Ile Lys Glu Thr Phe Gly Phe Asp Pro Lys Pro Phe Asp 
    530                 535                 540                 


Ile Val Lys Met Lys Leu Trp Arg Glu Gln Asn Gly Lys Cys Ala Tyr 
545                 550                 555                 560 


Ser Ser Lys Pro Ile Pro Ala Glu Arg Leu Tyr Glu Glu Asn Phe Val 
                565                 570                 575     


Gln Val Asp His Ile Ile Pro Phe Ser Arg Cys Phe Asp Asp Ser Tyr 
            580                 585                 590         


Gln Asn Lys Val Leu Val Leu Thr Asp Glu Asn Gln Lys Lys Lys Glu 
        595                 600                 605             


Arg Thr Pro Phe Glu Tyr Phe Gly Glu Asp Lys Glu Arg Trp His Gln 
    610                 615                 620                 


Phe Glu Glu Phe Val Asn Leu Thr Tyr Lys Phe Asn Thr Arg Lys Lys 
625                 630                 635                 640 


Glu Asn Leu Leu Ile Lys Asn Phe Asn Glu Glu Lys Ser Lys Glu Trp 
                645                 650                 655     


Ile Gln Arg Asn Ile Asn Asp Thr Lys Tyr Ile Ser Lys Phe Met Tyr 
            660                 665                 670         


Asn Tyr Ile Ala Asn Asn Leu Lys Phe Ala Asp Ser Glu Ser Lys Arg 
        675                 680                 685             


Lys Val Tyr Asn Ile Asn Gly Gln Ala Thr Ser Ile Leu Arg His Tyr 
    690                 695                 700                 


Trp Gly Leu Lys Lys Asp Arg Glu Glu Ser Asp Lys His His Ala Gln 
705                 710                 715                 720 


Asp Ala Val Ile Ile Ala Cys Ala Thr Asn Lys Asn Ile Lys Lys Val 
                725                 730                 735     


Ser Asp Tyr Ser Arg Arg Lys Leu Leu Tyr Lys Asn Asn Glu Ile Ile 
            740                 745                 750         


Asp Glu Glu Thr Gly Glu Ile Ile Asp Lys Lys Tyr Asn Ile Asp Ile 
        755                 760                 765             


Ser Ile Lys Glu Pro Trp Pro Lys Phe Arg Glu Glu Val Glu Ala Arg 
    770                 775                 780                 


Met Glu Asp Ala Asp Asn Asn Gly Gln Leu Tyr Ala Leu Ile Asn Gly 
785                 790                 795                 800 


Asn Phe Thr Asn Tyr Asp Asp Val Asp Ile Thr His Ile Lys Pro Ile 
                805                 810                 815     


Phe Thr Ser Arg Met Pro Glu Arg Lys Ile Thr Gly Arg Ala His Lys 
            820                 825                 830         


Asp Thr Met Arg Ser Gln Lys Phe Ile Gln Gln Gly Gln Asn Phe Thr 
        835                 840                 845             


Val Val Lys Lys Pro Leu Lys Ala Ile Ser Lys Gln Glu Ile Glu Gly 
    850                 855                 860                 


Ile Ile Asn Asn Pro Asp Phe Lys Asn Leu Tyr Leu Ser Asp Lys Lys 
865                 870                 875                 880 


Leu Tyr Asp Asp Ile Tyr Gln Lys Met Lys Glu Ala Asp Phe Lys Ala 
                885                 890                 895     


Glu Lys Ala Phe Ala Asn Glu Tyr Arg Lys Tyr Ser Lys Asn Gly Asn 
            900                 905                 910         


Ser Pro Ile Val Arg Ser Ile Lys Val Ala Ser Ala Gly Ala Thr Gly 
        915                 920                 925             


Val Gln Leu Lys Asn Gly Ala Ile Ala Glu Asn Ala Asn Met Val Arg 
    930                 935                 940                 


Val Asp Val Phe Glu Lys Asp Asn Lys Asn Tyr Leu Val Pro Ile Tyr 
945                 950                 955                 960 


Val Ser Asp Phe Ala Lys Asn Glu Leu Pro Asn Lys Ala Ile Cys Ala 
                965                 970                 975     


Asn Lys Asp Glu Lys Glu Trp Ile Glu Met Thr Glu Lys Tyr Lys Phe 
            980                 985                 990         


Lys Phe Ser Leu Tyr Pro Asn Asp  Leu Val Lys Ile Lys  Arg Lys Asn 
        995                 1000                 1005             


Glu Lys  Glu Phe Tyr Ala Tyr  Tyr Cys Ser Thr His  Arg Gly Thr 
    1010                 1015                 1020             


Gly Asn  Ile Asn Leu Leu Leu  Val Asn Gly Glu Ala  Arg Lys Asp 
    1025                 1030                 1035             


Gly Val  Gly Ile Lys Asn Thr  Glu Ile Phe Glu Lys  Tyr Gln Val 
    1040                 1045                 1050             


Asp Val  Leu Gly Asn Ile Ser  Lys Val Asn Lys Glu  Lys Arg Glu 
    1055                 1060                 1065             


Gly Val  Lys 
    1070     


<210>  576
<211>  1071
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  576

Met Asp Tyr Arg Ile Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ser Ile Ile Glu Leu Thr Thr Val Gln Ser Lys Leu Asp Gly Lys Tyr 
            20                  25                  30          


Arg Leu Lys Ala Val Gly Leu Ile Asp Ala Gly Val Arg Met Phe Asp 
        35                  40                  45              


Arg Ala Glu Val Pro Lys Thr Gly Ala Ser Leu Ala Leu Pro Arg Arg 
    50                  55                  60                  


Leu Ala Arg Ser Ser Arg Arg Arg Leu Thr Arg Arg Ser Ala Arg Lys 
65                  70                  75                  80  


Lys Ala Ile Arg Asn Leu Leu Val Lys Glu Asp Ile Leu Asn Gln Ile 
                85                  90                  95      


Glu Leu Asp His Leu Tyr Arg Thr Ser Glu Leu Val Asp Ile Trp Gln 
            100                 105                 110         


Ile Arg Val Glu Gly Leu Glu Arg Arg Leu Gly Arg Gln Glu Trp Ala 
        115                 120                 125             


Arg Leu Leu Ile His Leu Gly Gln Lys Arg Gly Tyr Arg Ser Thr Arg 
    130                 135                 140                 


Lys Ala Glu Glu Gly Ala Asp Thr Glu Gly Arg Ala Val Leu Ser Ser 
145                 150                 155                 160 


Ile Lys Glu Asn Gln Glu Thr Leu Lys Gln Tyr Arg Thr Val Gly Glu 
                165                 170                 175     


Met Trp Ala Thr Asp Asp Lys Phe Lys Gly Arg Val Arg Asn Ser Thr 
            180                 185                 190         


Asn Glu Phe Ile Phe Asn Val Ser Arg Asp Asn Leu Glu Glu Glu Ile 
        195                 200                 205             


Arg Phe Leu Phe Gln Gln Gln Arg Glu Leu Gly Ser Met Tyr Ala Ser 
    210                 215                 220                 


Lys Leu Leu Glu Glu Ser Tyr Met Glu Val Trp Gly His Gln Leu Pro 
225                 230                 235                 240 


Phe Ala Ser Gly Glu Ser Ile Leu Lys Asn Val Gly Thr Cys Ser Leu 
                245                 250                 255     


Tyr Glu Lys Glu Lys Arg Ala Ala Lys Ala Thr Tyr Thr Phe Gln Tyr 
            260                 265                 270         


Phe Met Ala Leu Asp Lys Leu Asn Asn Val Arg Ile Leu Pro Ser Met 
        275                 280                 285             


Arg Thr Leu Ser Glu Glu Glu Arg Gly Leu Ala Leu Lys Ala Met Phe 
    290                 295                 300                 


Glu Arg Asp Lys Leu Pro Lys Asn Pro Pro Lys Phe Thr Tyr Ser His 
305                 310                 315                 320 


Leu Arg Lys Ala Ile Lys Leu Glu Glu Asp Glu Thr Phe Gln Thr Ile 
                325                 330                 335     


Ser Tyr Asp Val Glu Lys Thr Leu Ala Thr Asn Glu Ser Ala Val Phe 
            340                 345                 350         


Val Asn Leu Ala Ala Tyr Tyr Gln Ile Lys Lys Val Cys Asp Glu His 
        355                 360                 365             


Asn Glu Asn Tyr Asp Tyr Val Asp Tyr Asn Thr Ile Gly His Ala Leu 
    370                 375                 380                 


Thr Val Tyr Lys Thr Asp Glu Asp Ile Asn Lys Tyr Leu Ala Ser Lys 
385                 390                 395                 400 


Asn Asn Ile Ala Lys Arg Val Tyr Ala Asn Asp Phe Ile Asp Ser Val 
                405                 410                 415     


Leu Lys His Ser Tyr Thr Thr Phe Gly His Leu Ser Leu Lys Ala Leu 
            420                 425                 430         


Ser Arg Ile Ile Pro Leu Met Glu Glu Gly Glu Val Phe Ala Lys Ala 
        435                 440                 445             


Lys Glu Lys Ala Gly Phe Lys Glu Glu Ser Tyr Leu Glu Ile Lys Ser 
    450                 455                 460                 


Lys Leu Leu Thr Asn Ile Pro Asp Ala Ile Lys Asn Pro Val Val Met 
465                 470                 475                 480 


Arg Ser Leu Thr Gln Ala Arg Lys Val Ile Asn Ala Ile Ile Lys Arg 
                485                 490                 495     


Met Gly Glu Gln Pro Ala Ser Ile Gln Ile Glu Leu Ala Arg Glu Leu 
            500                 505                 510         


Ser Lys Asp Val Ser Glu Arg Lys Lys Ile Thr Asn Gly Phe Ala Glu 
        515                 520                 525             


Asn Arg Ser Arg Asn Gln Phe Ala Ile Lys Thr Leu Glu Glu Asn His 
    530                 535                 540                 


Ile Thr Asn Ile Arg Gly Gln Asp Ile Val Lys Phe Lys Leu Trp Gln 
545                 550                 555                 560 


Glu Gln Gly Gly Arg Cys Ala Tyr Ser Leu Lys Pro Ile Lys Leu Glu 
                565                 570                 575     


Glu Leu Ile Ser Phe Leu Lys Lys Glu Arg Arg Tyr Glu His Asn Leu 
            580                 585                 590         


Asp Val Asp His Ile Ile Pro Tyr Ser Lys Thr Phe Asp Asp Ser Tyr 
        595                 600                 605             


Thr Asn Lys Val Leu Val Phe Ser Asp Glu Asn Arg Lys Lys Gly Asp 
    610                 615                 620                 


Gln Ile Pro Ala Glu Tyr Leu Gly Ile Gly Thr Lys Arg Trp Glu Lys 
625                 630                 635                 640 


Tyr Lys Glu Phe Val Ile Thr Ser Lys Phe Pro Ala Ser Lys Lys Lys 
                645                 650                 655     


Arg Leu Leu Lys Glu Gln Leu Val Glu Arg Glu Glu Leu Phe Met Lys 
            660                 665                 670         


Glu Arg His Leu Asn Asp Thr Arg Tyr Ala Ser Arg Tyr Phe Met Asn 
        675                 680                 685             


Phe Leu Arg Glu Asn Leu Leu Arg Lys Glu Thr Val Leu His Glu Thr 
    690                 695                 700                 


Gln Trp Val Thr Ala Val Ala Gly Arg Val Thr Ser His Ile Arg Lys 
705                 710                 715                 720 


Arg Leu Gly Phe Gly Lys Asp Arg Glu Glu Thr His Leu His His Ala 
                725                 730                 735     


Leu Asp Ala Ile Ile Val Ala Cys Thr Asp Lys Trp Met Ile Asp Cys 
            740                 745                 750         


Val Thr Asn Tyr Tyr Lys Ser Arg Glu Gly Gly Ser Ile Lys Arg Lys 
        755                 760                 765             


Glu Ile Arg Phe Pro Ser Pro Trp Asn Gly Phe Trp His Asp Val Gln 
    770                 775                 780                 


Thr Lys Leu Ala Gln Leu Pro Gln Pro Glu Val Ile Asn Glu Ser Ile 
785                 790                 795                 800 


Ser Lys Gly Asn Pro Asp Ala Val Asp Tyr Met Leu Val Ser Arg Met 
                805                 810                 815     


Pro Arg Lys Ser Ile Thr Gly Ala Ala His Lys Glu Thr Ile Ser Lys 
            820                 825                 830         


Trp Gly Gly Tyr Asp Asp Lys Gly Lys Ala Leu Leu Val Lys Lys Val 
        835                 840                 845             


Ala Leu Ser Glu Leu Lys Phe Lys Asn Asp Asp Phe Pro Met Tyr Asn 
    850                 855                 860                 


Lys Glu Thr Asp Pro Leu Leu Tyr Glu Ala Ile Lys Asn Arg Tyr Val 
865                 870                 875                 880 


Glu Ser Gly Arg Asp Val Lys Arg Ala Phe Ala Thr Pro Leu Tyr Lys 
                885                 890                 895     


Pro Ser Lys Ser Gly Lys Arg Asn Glu Val Lys Arg Val Lys Val Ile 
            900                 905                 910         


Ala Glu Lys Lys Ala Met Val Arg Glu Val Asn Gly Gly Ile Ala Glu 
        915                 920                 925             


Asn Gly Gly Leu Val Arg Ser Asp Ile Phe Leu Lys Glu Lys Lys Tyr 
    930                 935                 940                 


Val Met Val Pro Val Tyr Thr Phe Asp Thr Ile Lys Gln Gln Leu Pro 
945                 950                 955                 960 


Asn Lys Tyr Val Val Ser Gly Arg Gly Tyr Glu Gln Trp Pro Glu Leu 
                965                 970                 975     


Thr Ala Glu His Val Phe Gln Phe Ser Val Tyr Pro Tyr Glu Leu Val 
            980                 985                 990         


His Val Gly Thr Asn Lys Gly Asp  Asn Ser Phe Tyr Tyr  Phe Ala Ser 
        995                 1000                 1005             


Leu Asn  Ile Ser Ser Asn Val  Leu Lys Phe Ser Glu  Cys Asn Arg 
    1010                 1015                 1020             


Pro Thr  Lys Asp Tyr Ile Glu  Ile Gly Leu Asn Ser  Ile Ile Leu 
    1025                 1030                 1035             


Leu Glu  Lys Tyr Glu Ile Asp  Val Leu Gly Arg Ile  Thr Gly Gln 
    1040                 1045                 1050             


Ser Lys  Lys Gly Ile Arg Gln  Ser Phe Lys Asn Asn  Lys Ser Gln 
    1055                 1060                 1065             


Met Lys  Glu 
    1070     


<210>  577
<211>  1072
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  577

Met Arg Leu Gly Leu Asp Ile Gly Thr Asn Ser Val Gly Trp Trp Leu 
1               5                   10                  15      


Tyr Lys Thr Asp Gly Glu Ala Ala Ser Ala Arg Ile Val Glu Val Ile 
            20                  25                  30          


Asp Gly Gly Val Arg Ile Phe Ser Asp Gly Arg Asp Pro Lys Ser Lys 
        35                  40                  45              


Thr Ser Leu Ala Val Asp Arg Arg Val Ala Arg Ala Ala Arg Arg Arg 
    50                  55                  60                  


Arg Asp Arg Tyr Leu Arg Arg Arg Ala Ser Leu Met Lys Leu Leu Ala 
65                  70                  75                  80  


Arg Thr Gly Leu Met Pro Ala Asp Pro Val Glu Ala Lys Lys Leu Glu 
                85                  90                  95      


Arg Leu Asp Pro Tyr Glu Leu Arg Ser Thr Gly Leu Asp Asp Ala Leu 
            100                 105                 110         


Pro Leu Thr His Phe Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg 
        115                 120                 125             


Gly Phe Lys Ser Asn Arg Lys Thr Asp Arg Gly Asp Asn Glu Asn Gly 
    130                 135                 140                 


Lys Ile Arg Glu Ala Thr Ala Arg Leu Asp Leu Glu Met Met Thr Ser 
145                 150                 155                 160 


Gly Ala Arg Ser Tyr Gly Glu Phe Leu His Met Arg Arg Gln Arg Ala 
                165                 170                 175     


Ser Asp Pro Arg His Val Pro Ser Val Arg Thr Arg Leu Ser Val Ala 
            180                 185                 190         


Ala Arg Gly Gly Pro Asp Ala Lys Ala Glu Ala Gly Tyr Asp Phe Tyr 
        195                 200                 205             


Pro Asp Arg His His Leu Glu Glu Glu Phe His Ala Leu Trp Ala Ala 
    210                 215                 220                 


Gln Ala Thr His His Gln Ala Leu Thr Asp Asp Leu Arg Asp Leu Val 
225                 230                 235                 240 


Phe Glu Lys Ile Phe Tyr Gln Arg Pro Leu Lys Glu Pro Lys Ile Gly 
                245                 250                 255     


Leu Cys Leu Phe Thr Ala Glu Glu Arg Ile Ala Lys Ala His Pro Leu 
            260                 265                 270         


Thr Gln Arg Arg Val Leu Phe Glu Thr Ile Asn Gln Leu Arg Val Thr 
        275                 280                 285             


Ala Asp Gly Arg Ser Thr Arg Pro Leu Thr Leu Glu Glu Arg Asp Thr 
    290                 295                 300                 


Leu Val His Ala Leu Asp Asn Lys Lys Pro Thr Lys Ser Met Thr Ser 
305                 310                 315                 320 


Met Asn Met Thr Leu Lys Ala Leu Ala Lys Leu Leu Lys Leu Arg Asp 
                325                 330                 335     


Gly Glu Arg Phe Thr Leu Glu Thr Gly Val Arg Glu Asn Ile Ser Cys 
            340                 345                 350         


Asp Pro Val Arg Ala Cys Met Ala His Pro Ala Arg Phe Gly Pro Gln 
        355                 360                 365             


Trp Ser Ser Leu Asp Trp Glu Ala Gln Trp Asp Val Ile Ser Arg Val 
    370                 375                 380                 


Arg Gln Val Gln Ser Glu Gly Glu Phe Ile Glu Leu Thr Asp Trp Leu 
385                 390                 395                 400 


Ile Gln Gly His Gly Leu Asp Ala Asp His Ala Lys Ala Val Gly Asn 
                405                 410                 415     


Ala Pro Leu Pro Glu Gly Tyr Ala Arg Leu Gly Phe Ser Ala Thr Glu 
            420                 425                 430         


Arg Ile Leu Asp Gln Leu Lys Ala Glu Val Cys Thr Tyr Ser Asp Ala 
        435                 440                 445             


Val Ala Lys Cys Gly Trp His His Ser Asp Gln Arg Thr Gly Ala Cys 
    450                 455                 460                 


Leu Glu Val Leu Pro Tyr Tyr Gly Glu Val Leu Asp Arg His Val Ile 
465                 470                 475                 480 


Pro Gly Thr Tyr Asp Ala Thr Asp Asp Glu Ile Thr Arg Tyr Gly Arg 
                485                 490                 495     


Ile Thr Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu Arg Arg Leu 
            500                 505                 510         


Val Asn Lys Ile Ile Glu Val His Gly Lys Pro Asp Gln Ile Val Val 
        515                 520                 525             


Glu Leu Ala Arg Glu Leu Lys Gln Ser Glu Pro Gln Lys Lys Asp Ala 
    530                 535                 540                 


Met Lys Arg Leu Arg Asn Thr Thr Ala Asp Ala Ile Arg Arg Ser Glu 
545                 550                 555                 560 


Gln Leu Glu Gln Leu Gly Gln Pro Asn Thr Gly His Asn Arg Met Leu 
                565                 570                 575     


Leu Arg Leu Trp Glu Asp Leu Gly Pro Ala Ile Gly Pro Arg Cys Cys 
            580                 585                 590         


Pro Tyr Thr Gly Lys Thr Ile Ser Val Thr Met Val Phe Asp Gly Ser 
        595                 600                 605             


Cys Asp Val Asp His Ile Leu Pro Phe Ser Arg Thr Leu Asp Asp Ser 
    610                 615                 620                 


Phe Ala Asn Arg Thr Leu Cys Leu Lys Glu Ala Asn Arg Glu Lys Ser 
625                 630                 635                 640 


Asp Arg Thr Pro Trp Glu Ala Trp Gly Gly Thr Asp Gln Trp Asp Asp 
                645                 650                 655     


Ile Ala Ala Asn Leu Lys Asn Leu Pro Glu Lys Lys Arg Lys Arg Phe 
            660                 665                 670         


Gly Pro Asp Ala Met Glu Trp Phe Glu Gly Glu Asn Asp Phe Leu Asp 
        675                 680                 685             


Arg Ala Leu Val Asp Thr Gln Tyr Leu Ala Lys Ile Ser Arg Met Tyr 
    690                 695                 700                 


Leu Asp Thr Leu Phe Thr Lys Gly Gly His Val Trp Val Val Pro Gly 
705                 710                 715                 720 


Arg Met Thr Glu Met Leu Arg Arg His Trp Gly Leu Asn Ala Leu Leu 
                725                 730                 735     


Asn Asp Lys Asp Arg Gly Ala Val Lys Ala Lys Asn Arg Thr Asp His 
            740                 745                 750         


Arg His His Ala Ile Asp Ala Ala Val Val Ala Ala Thr Asp Tyr Arg 
        755                 760                 765             


Leu Met Gln Lys Ile Ser Arg Ala Ala Gly Gln Gly Glu Asn Ala Gly 
    770                 775                 780                 


Gln Ser Ala Glu Leu Ile Ala Arg Asp Thr Asp Ala Pro Trp Glu His 
785                 790                 795                 800 


Phe Arg Ala Asp Ile Ala Ser Gln Leu Glu Lys Ile Ile Val Ser His 
                805                 810                 815     


Arg Ala Asp His Gly Arg Ile Asp Val Glu Gly Arg Lys Thr Gly Arg 
            820                 825                 830         


Asp Ser Thr Ala Gly Arg Leu His Asn Asp Thr Ala Tyr Gly Val Ile 
        835                 840                 845             


Asp Asp Leu Thr Val Ala Ser Arg Thr Pro Leu Met Ser Leu Lys Pro 
    850                 855                 860                 


Ser Asp Ile Ala Ile Thr Glu Arg Gly Lys Asn Ile Arg Asp Pro Gln 
865                 870                 875                 880 


Leu Gln Asn Ala Leu Tyr Val Ala Thr Lys Gly Lys Glu Gly Lys Met 
                885                 890                 895     


Phe Glu Gln Ala Leu Val Asp Phe Ala Ser Lys Glu Gly Pro Tyr Gln 
            900                 905                 910         


Gly Ile Arg Arg Val Arg Leu Val Glu Thr Leu Gln Lys Ala Ala Arg 
        915                 920                 925             


Val Glu Ile Gly Lys Thr Glu Ser Gly Gln Pro Leu Lys Ala Tyr Lys 
    930                 935                 940                 


Gly Asp Ser Asn His Cys Tyr Glu Leu Trp Lys Leu Pro Asp Gly Lys 
945                 950                 955                 960 


Ile Val Pro Gln Val Val Thr Thr Tyr Glu Ala His Ala Gly Ser Ala 
                965                 970                 975     


Pro Arg Pro His Pro Ala Ala Lys Arg Ile Leu Arg Val Phe Lys Lys 
            980                 985                 990         


Asp Met Val Lys Leu Thr Lys Asp  Gly Glu Glu Ile Val  Gly Tyr Val 
        995                 1000                 1005             


Gln Ser  Met His Ile Lys Asn  Gly Leu Phe Val Ala  Gln His Leu 
    1010                 1015                 1020             


Glu Ala  Asn Ala Asp Ala Arg  Asn Arg Asp Lys Thr  Asp Pro Phe 
    1025                 1030                 1035             


Lys Phe  Leu Gln Met Gly Ala  Gly Thr Leu Val Lys  Thr Gly Leu 
    1040                 1045                 1050             


Arg Arg  Val Leu Val Asp Glu  Ile Gly Arg Leu Arg  Asp Pro Gly 
    1055                 1060                 1065             


Pro Pro  Lys Leu 
    1070         


<210>  578
<211>  1074
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  578

Met Gln Thr Lys Asn Leu Pro Tyr Thr Leu Gly Leu Asp Ile Gly Met 
1               5                   10                  15      


Ala Ser Val Gly Ala Ala Leu Leu Leu Pro Asp Gln Glu Arg Ile Leu 
            20                  25                  30          


Ala Leu His Val Arg Thr Phe Asp Lys Ala Glu Thr Ala Lys Glu Gly 
        35                  40                  45              


Glu Ser Leu Asn Lys Ile Arg Arg Asp Ser Arg Leu Thr Arg Arg Arg 
    50                  55                  60                  


Ile Arg Arg Arg Ala His Arg Leu Val Arg Leu Ala Arg Leu Met Lys 
65                  70                  75                  80  


Arg Val Gly Leu Ile Ala Asp Ala Asn Pro Glu Ala Phe Ala Leu Pro 
                85                  90                  95      


Gly Val Ser Pro Trp His Leu Arg Ala Asp Gly Leu Asp Arg Leu Leu 
            100                 105                 110         


Glu Pro Lys Glu Trp Ala Gly Val Leu Tyr His Leu Val Lys His Arg 
        115                 120                 125             


Gly Phe Gln Ser Asn Arg Lys Ser Glu Ala Lys Ala Asp Glu Lys Ala 
    130                 135                 140                 


Gly Gln Met Leu Ser Gly Val Gly Arg Asn Gln Ala Leu Leu Lys Glu 
145                 150                 155                 160 


Ala Gly Tyr Arg Thr Val Gly Glu Leu Ala Ala Arg His Glu Asp Phe 
                165                 170                 175     


Lys Glu Ala Lys Arg Asn Lys Gly Gly Ser Tyr Ser His Thr Phe Ala 
            180                 185                 190         


Arg Ala Asp Leu Asn Ala Glu Leu Asn Leu Leu Phe Glu Arg Gln Arg 
        195                 200                 205             


Ala Leu Gly Ser Arg Leu Ala Ser Ala Asp Phe Gln Glu Asn Val Arg 
    210                 215                 220                 


Asp Leu Leu Met Ala Arg Arg Pro Thr Leu Ser Gly Ala Asn Leu Leu 
225                 230                 235                 240 


Lys Met Val Gly Lys Cys Thr Phe Glu Lys Ser Glu Asp Arg Ala Pro 
                245                 250                 255     


Lys Ala Gly Tyr Arg Ala Glu Arg Phe Val Trp Leu Gly Lys Leu Asn 
            260                 265                 270         


Asn Leu Lys Ile Val Gly Ser Gly Asp Ala Arg Pro Leu Thr Asp Ala 
        275                 280                 285             


Glu His Arg Ile Val Ile Asp Leu Pro Phe Thr Gln Ala Lys Leu Thr 
    290                 295                 300                 


Phe Lys Gln Val Arg Lys Ala Leu Glu Leu Glu Thr His Gln Arg Phe 
305                 310                 315                 320 


Asn Leu Leu Ser Tyr Arg Pro Asp Pro Lys Gly Lys Asp Lys Asp Pro 
                325                 330                 335     


Glu Glu Ser Thr Phe Phe Glu Ala Lys Ser Phe His Thr Leu Arg Lys 
            340                 345                 350         


Ala Tyr Glu Gly Ala Gly Leu Lys Lys Glu Trp Gln Arg Asp Ala Leu 
        355                 360                 365             


Asp Pro Asp Arg Leu Asp Ala Leu Ala Tyr Ala Leu Thr Cys Tyr Lys 
    370                 375                 380                 


Asp Asp Thr Glu Ser Arg Gln Trp Leu Val Ala Gln Gly Ile Gly Thr 
385                 390                 395                 400 


Pro Ile Ile Glu Ala Val Leu Ala Glu Ser Phe Asp Lys Phe Ile His 
                405                 410                 415     


Leu Ser Gln Lys Ala Leu Lys Ala Ile Leu Pro His Met Glu Ala Gly 
            420                 425                 430         


Gln Arg Tyr Asp Asp Ala Ala Lys Ser Ala Gly Tyr Asp His Ser Gln 
        435                 440                 445             


Pro Asp Ala Gly Thr Gly Lys Arg Ala Thr Leu Pro Pro Pro Asp Lys 
    450                 455                 460                 


Asp Thr Ile Arg Asn Pro Val Val Tyr Arg Ala Leu Asn Gln Ala Arg 
465                 470                 475                 480 


Lys Leu Val Asn Ala Ile Val Ala Glu Tyr Gly Pro Pro Ala Ala Val 
                485                 490                 495     


His Ile Glu Leu Ala Arg Asp Leu Ser Lys Pro Ile Asp Glu Arg Arg 
            500                 505                 510         


Asp Ile Glu Lys Gly Gln Lys Glu Phe Gln Ala Leu Lys Glu Lys Leu 
        515                 520                 525             


Ala Ala Ala Phe Glu Glu Thr Phe His Arg Lys Pro Ser Gly Leu Asp 
    530                 535                 540                 


Leu Leu Lys Tyr Arg Leu Phe Arg Glu Gln Leu Asp Gln Cys Pro Tyr 
545                 550                 555                 560 


Cys Gln Lys Ala Leu Val Val Asp Arg Leu Phe Glu Pro Gly Tyr Ala 
                565                 570                 575     


Gln Ile Asp His Ala Leu Pro Tyr Ser Arg Ser Phe Asp Asp Gly Met 
            580                 585                 590         


Asn Asn Lys Val Val Val His Val Ala Cys Asn Gln Asp Lys Gly Asn 
        595                 600                 605             


Arg Thr Pro Tyr Glu Phe Leu Asp Gly Ala Gly Asp Ser Glu Arg Trp 
    610                 615                 620                 


Gln Arg Phe Val Ala Trp Val Gln Gly Asn Lys Ala Ile Arg Gln Ala 
625                 630                 635                 640 


Lys Arg Asn Arg Leu Leu Arg Val Asn Phe Gly Thr Asp Glu Ala Lys 
                645                 650                 655     


Glu Phe Ser Lys Arg Asn Leu Ser Asp Thr Arg Tyr Ile Cys Arg Glu 
            660                 665                 670         


Phe Lys Arg Thr Ile Glu Thr Gln Phe Ala Trp His Ser Asp Ala Glu 
        675                 680                 685             


Gly Asn Glu Arg Cys Val Val Leu Ser Gly Gln Leu Thr Ser Leu Leu 
    690                 695                 700                 


Arg Ala Arg Trp Gly Leu Ile Lys Val Arg Glu Asp Gly Asp Leu His 
705                 710                 715                 720 


His Ala Leu Asp Val Ala Val Ile Ala Ala Ala Ser Arg Ser Leu Val 
                725                 730                 735     


Lys Arg Met Ala Asp Tyr Ser Arg Arg Asn Glu Leu Glu Met Val Arg 
            740                 745                 750         


Asn Arg Tyr Val Asp Pro Ala Thr Gly Glu Val Val Asp Val Ala Ala 
        755                 760                 765             


Leu Arg Gln Ile Glu Asp His Phe Pro Lys Pro Trp Pro Leu Phe Arg 
    770                 775                 780                 


Glu Glu Leu Leu Ala Arg Leu Ala Pro Glu Pro Lys Leu Gln Leu Thr 
785                 790                 795                 800 


Cys Val Ser Ser Tyr Asp Glu Gly Leu Arg Glu Ser Ile Arg Pro Ile 
                805                 810                 815     


Arg Val Ser Arg Ala Pro Thr Arg Arg Gly Leu Gly Ala Ala His Gln 
            820                 825                 830         


Glu Thr Ile Arg Ser Leu Arg Asp Gly Ser Gln Ser Ala Ile Lys Thr 
        835                 840                 845             


Pro Leu Arg Asp Leu Asn Leu Lys Asp Leu Glu Asn Ile Val Gly Phe 
    850                 855                 860                 


Glu Arg Asp Thr Ala Leu Ile Asp Ala Ile Arg Thr Arg Leu Glu Ala 
865                 870                 875                 880 


Phe Gly Gly Glu Gly Lys Lys Ala Phe Asp Asp Lys Gln Pro Pro Leu 
                885                 890                 895     


Arg Lys Pro Ser Lys Asp Pro Asp Lys Ala Pro Ile Ile Arg Ser Val 
            900                 905                 910         


Lys Leu Leu Val Thr Gln Lys Ser Gly Ile Pro Ile Arg Gly Gly Ile 
        915                 920                 925             


Ala Asn Asn Gly Ala Met Leu Arg Ser Asp Ile Phe Thr Lys Ala Gly 
    930                 935                 940                 


Lys Phe Tyr Ala Val Pro Ile Tyr Val Ala Asp Ala Val Arg Gly Glu 
945                 950                 955                 960 


Leu Pro His Arg Ala Val Ala Gln Ala Lys Pro Glu Leu Glu Trp Pro 
                965                 970                 975     


Met Met Asp Glu Ser Tyr Gln Phe Leu Phe Ser Leu His Pro Asn Asp 
            980                 985                 990         


Trp Met Thr Ile Arg Leu Lys Asn  Glu Val Arg Glu Gly  Tyr Tyr Ala 
        995                 1000                 1005             


Gly Leu  Asp Arg Ala Thr Gly  Asn Ile Ser Leu Trp  Leu His Asp 
    1010                 1015                 1020             


Arg Asn  Gln Asn Val Gly Gln  Lys Gly Leu Leu Thr  Gly Asn Gly 
    1025                 1030                 1035             


Ile Lys  Thr Ala Leu Ala Val  Glu Lys Tyr His Val  Asp Leu Leu 
    1040                 1045                 1050             


Gly Lys  Leu His Arg Val His  Lys Glu Glu Arg Gln  Pro Ile His 
    1055                 1060                 1065             


Ile Lys  Pro Gly Lys Arg 
    1070                 


<210>  579
<211>  1077
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  579

Met Lys Tyr Ser Ile Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Glu Asp Ile Glu Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Val Ala Glu Gln Pro Lys Thr Gly Ala Ser Leu Ser Thr 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Thr Arg Lys Arg Leu Arg Arg Arg Arg 
    50                  55                  60                  


His Arg Val Glu Arg Val Arg Lys Phe Met Ile Gln Lys Gly Leu Leu 
65                  70                  75                  80  


Thr Gln Val Gln Met Glu Gln Leu Tyr Asp Trp Lys Asp Gly Asp Leu 
                85                  90                  95      


Asp Ile Trp Leu Val Arg Val Asn Ala Leu Glu Arg Lys Leu Ser Asp 
            100                 105                 110         


Arg Glu Phe Ala Arg Ile Leu Val His Phe Ala Lys Asn Arg Gly Phe 
        115                 120                 125             


Gln Ser Asn Arg Lys Ser Glu Leu Gln Glu Glu Asp Asn Gly Ala Val 
    130                 135                 140                 


Leu Ser Ala Val Gln Glu Asn Lys Arg Leu Met Glu Glu Lys Gly Tyr 
145                 150                 155                 160 


Gln Thr Ile Ser Glu Met Leu Leu Tyr Asp Glu Lys Phe Glu Gly Thr 
                165                 170                 175     


Lys Arg Asn Lys Gly Gly Glu Tyr Thr His Val Val Ala Arg Ser Asp 
            180                 185                 190         


Leu Lys Arg Glu Ile Gln Leu Ile Phe Glu Lys Gln Arg Ala Tyr Gly 
        195                 200                 205             


His Ala Phe Ala Thr Met Glu Asn Glu Asp Lys Phe Leu Gln Ile Trp 
    210                 215                 220                 


Ser Ser Gln Arg Pro Phe Ala Thr Lys Asp Asp Ile Ala Lys Lys Ile 
225                 230                 235                 240 


Gly Asn Cys Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ser Thr 
                245                 250                 255     


Tyr Thr Phe Glu Arg Phe Arg Ala Leu Asp Lys Leu Asn Arg Leu Arg 
            260                 265                 270         


Ile Leu Ser Gly Thr Ser Pro Ala Arg Ser Leu Thr Gln Glu Glu Arg 
        275                 280                 285             


Asp Leu Ile Leu Asn Ser Leu Phe Ser Lys Lys Glu Val Lys Tyr Lys 
    290                 295                 300                 


Glu Leu Arg Lys Leu Leu Lys Leu Asn Asp Glu Gln Arg Phe Asn Glu 
305                 310                 315                 320 


Ile Phe Tyr Asp Pro Asn Glu Lys Ile Glu Lys Asn Glu Asn Arg Thr 
                325                 330                 335     


Phe Leu Ser Leu Glu Ser Gln Tyr Lys Ile Arg Lys Ile Leu Gln Lys 
            340                 345                 350         


Ile Glu Gly Lys Ser Leu Asn Asp Thr Tyr Arg Pro Val Asp Leu Asp 
        355                 360                 365             


Thr Ile Ala Tyr Ala Leu Thr Val Phe Lys Asp Asp Gln Asp Ile Arg 
    370                 375                 380                 


Asp Tyr Leu Gln Asn Gln Tyr Val Ala Ser Asn Gly Lys Lys Glu Leu 
385                 390                 395                 400 


Asn Leu Ala Asn Arg Lys Tyr Asn Asp Glu Leu Ile Glu Glu Leu Leu 
                405                 410                 415     


His Leu Ser Phe Thr Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg 
            420                 425                 430         


Asn Ile Leu Pro Phe Met Glu Gln Gly Ile Pro Tyr His Gln Ala Cys 
        435                 440                 445             


Glu Lys Ala Gly Tyr Gln Phe Asn Lys Lys Val Asn Arg Glu Lys Gln 
    450                 455                 460                 


Lys Leu Leu Pro Pro Val Pro Ala Lys Asp Ile Val Asn Pro Val Val 
465                 470                 475                 480 


Ile Arg Ser Leu Ser Gln Val Arg Lys Val Ile Asn Gly Ile Ile Lys 
                485                 490                 495     


Arg Tyr Gly Ser Pro Ser Ser Ile Tyr Ile Glu Leu Ala Arg Glu Met 
            500                 505                 510         


Gly Arg Gly Tyr Lys Glu Arg Lys Asp Leu Glu Lys Gln Tyr Asn Lys 
        515                 520                 525             


Asn Arg Gln Met Asn Glu Gly Ala Lys Lys His Ile Leu Glu Leu Arg 
    530                 535                 540                 


Pro Glu Phe Gly Asp Pro Arg Gly His Asp Ile Leu Lys Tyr Lys Leu 
545                 550                 555                 560 


Trp Gln Glu Gln Asp Cys Arg Cys Ala Tyr Ser Phe Lys Ser Ile Pro 
                565                 570                 575     


Ile Lys Gln Leu Phe Glu Ile Gly Tyr Ala Glu Val Asp His Ile Ile 
            580                 585                 590         


Pro Tyr Ser Arg Ser Phe Asp Asp Ser Asn Asn Asn Lys Val Leu Val 
        595                 600                 605             


Leu Ala Ala Glu Asn Gln Asn Lys Gln Asn Arg Thr Pro Tyr Glu Trp 
    610                 615                 620                 


Phe Gly His Asp Glu Thr Lys Trp Gln Glu Phe Thr Ser Leu Val Thr 
625                 630                 635                 640 


Thr Met Lys Val Ser Lys Lys Lys Lys Ser Leu Leu Leu Lys Lys Asn 
                645                 650                 655     


Phe Asp Glu Glu Gln Ala Glu Glu Phe Arg Ser Arg His Leu Asn Asp 
            660                 665                 670         


Thr Arg Tyr Ile Thr Arg Tyr Ile Lys Thr Phe Ile Glu Asp Asn Leu 
        675                 680                 685             


Gln Phe Arg Ile Glu Glu Gly Lys Lys Thr Tyr Val His Thr Val Asn 
    690                 695                 700                 


Gly Ala Tyr Thr Ser Leu Met Arg Lys Arg Trp Gly Phe Asn Lys Asn 
705                 710                 715                 720 


Arg Gln Glu Asn Ala Leu His His Ala Leu Asp Ala Val Ile Val Ala 
                725                 730                 735     


Val Ser Leu Pro Phe Lys His Lys Val Arg Ala Tyr Phe Lys Arg Arg 
            740                 745                 750         


Glu Glu Tyr Ala His Asn Gln Leu Lys Arg Glu Gly Glu Phe Phe Pro 
        755                 760                 765             


Glu Pro Trp Pro Gly Phe Ile His Glu Leu Glu Ile Arg Met Met Gln 
    770                 775                 780                 


Asp Ser Glu Asn Ile Lys Leu Ala Met Glu Ser Leu Asn Leu Lys Thr 
785                 790                 795                 800 


Tyr Asp Glu Ser Phe Ile Lys Glu Leu Lys Pro Ile Phe Val Ser Arg 
                805                 810                 815     


Met Pro Lys Arg Ala Met Lys Gly Gln Ile His Glu Glu Thr Leu Lys 
            820                 825                 830         


Arg Val Arg Gly Arg Thr Asp Asp Gly Tyr Ile Arg Thr Val Lys Lys 
        835                 840                 845             


Val Lys Leu Asp Gln Ile Pro Phe Asp Lys Asn Gly Asp Phe Pro Met 
    850                 855                 860                 


Phe Gly Lys Glu Thr Asp Met Lys Thr Tyr Glu Ala Ile Lys Ser Arg 
865                 870                 875                 880 


Tyr Leu Glu Ser Gly Lys Asp Lys Lys Lys Ala Phe Asn Thr Pro Leu 
                885                 890                 895     


Tyr Lys Pro Ala Lys Asn Pro Glu Asn Ala Pro Ile Ile Arg Ser Ile 
            900                 905                 910         


Lys Ile Glu Glu Lys Ala Asn Arg Val Val Met Leu Asp Glu Lys Thr 
        915                 920                 925             


Ala Ala Asp Asn Ala Ser Ile Val Arg Thr Glu Val Phe Arg His Lys 
    930                 935                 940                 


Glu Thr Gly Asn Tyr Phe Leu Thr Pro Val Tyr Val Ala Asp Val Leu 
945                 950                 955                 960 


Ala Lys Lys Val Pro Asp Arg Leu Ile Thr Thr Lys Lys Pro Tyr Ser 
                965                 970                 975     


Glu Trp Arg Lys Ile Thr Asp Glu Tyr Glu Phe Leu Phe Ser Leu Tyr 
            980                 985                 990         


Pro Asn Asp Leu Ile Arg Val Lys  Met Pro Lys Glu Lys  Glu Thr Lys 
        995                 1000                 1005             


Thr His  Thr Gly Glu Lys His  Val Trp Gln Glu Asn  Phe Met Tyr 
    1010                 1015                 1020             


Phe Lys  Gly Val His Ser Gly  Ser Ala Gly Val Arg  Met Ile Asp 
    1025                 1030                 1035             


His Met  Asn Ser Phe Glu Asp  Glu Ile Gly Val Arg  Lys Leu Val 
    1040                 1045                 1050             


Val Phe  Glu Lys Tyr Gln Val  Asp Pro Leu Gly Arg  Met Asn Lys 
    1055                 1060                 1065             


Val His  Gly Glu Lys Arg His  Gly Ile 
    1070                 1075         


<210>  580
<211>  1077
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  580

Met Lys Tyr Ser Ile Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Glu Asp Ile Glu Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Val Ala Glu Gln Pro Lys Thr Gly Ala Ser Leu Ser Thr 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Thr Arg Lys Arg Leu Arg Arg Arg Arg 
    50                  55                  60                  


His Arg Val Glu Arg Val Arg Lys Phe Met Ile Gln Lys Gly Leu Leu 
65                  70                  75                  80  


Thr Gln Val Gln Met Glu Gln Leu Tyr Asp Trp Lys Asp Gly Asp Leu 
                85                  90                  95      


Asp Ile Trp Leu Val Arg Val Asn Ala Leu Glu Arg Lys Leu Ser Asp 
            100                 105                 110         


Arg Glu Phe Ala Arg Ile Leu Val His Phe Ala Lys Asn Arg Gly Phe 
        115                 120                 125             


Gln Ser Asn Arg Lys Ser Glu Leu Gln Glu Glu Asp Asn Gly Ala Val 
    130                 135                 140                 


Leu Ser Ala Val Gln Glu Asn Lys Arg Leu Met Glu Glu Lys Gly Tyr 
145                 150                 155                 160 


Gln Thr Ile Ser Glu Met Leu Leu Tyr Asp Glu Lys Phe Glu Gly Thr 
                165                 170                 175     


Lys Arg Asn Lys Gly Gly Glu Tyr Thr His Val Val Ala Arg Ser Asp 
            180                 185                 190         


Leu Lys Arg Glu Ile Gln Leu Ile Phe Glu Lys Gln Arg Ala Tyr Gly 
        195                 200                 205             


His Ala Phe Ala Thr Met Glu Asn Glu Asp Lys Phe Leu Gln Ile Trp 
    210                 215                 220                 


Ser Ser Gln Arg Pro Phe Ala Thr Lys Asp Asp Ile Ala Lys Lys Ile 
225                 230                 235                 240 


Gly Asn Cys Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ser Thr 
                245                 250                 255     


Tyr Thr Phe Glu Arg Phe Arg Ala Leu Asp Lys Leu Asn Arg Leu Arg 
            260                 265                 270         


Ile Leu Ser Gly Thr Ser Pro Ala Arg Ser Leu Thr Gln Glu Glu Arg 
        275                 280                 285             


Asp Leu Ile Leu Asn Ser Leu Phe Ser Lys Lys Glu Val Lys Tyr Lys 
    290                 295                 300                 


Glu Leu Arg Lys Leu Leu Lys Leu Asn Asp Glu Gln Arg Phe Asn Glu 
305                 310                 315                 320 


Ile Phe Tyr Asp Pro Asn Glu Lys Ile Glu Lys Asn Glu Asn Arg Thr 
                325                 330                 335     


Phe Leu Ser Leu Glu Ser Gln Tyr Lys Ile Arg Lys Ile Leu Gln Lys 
            340                 345                 350         


Ile Glu Gly Lys Ser Leu Asn Asp Thr Tyr Arg Pro Val Asp Leu Asp 
        355                 360                 365             


Thr Ile Ala Tyr Ala Leu Thr Val Phe Lys Asp Asp Gln Asp Ile Arg 
    370                 375                 380                 


Asp Tyr Leu Gln Asn Gln Tyr Val Ala Ser Asn Gly Lys Lys Glu Leu 
385                 390                 395                 400 


Asn Leu Ala Asn Arg Lys Tyr Asn Asp Glu Leu Ile Glu Glu Leu Leu 
                405                 410                 415     


His Leu Ser Phe Thr Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg 
            420                 425                 430         


Asn Ile Leu Pro Phe Met Glu Gln Gly Ile Pro Tyr His Gln Ala Cys 
        435                 440                 445             


Glu Lys Ala Gly Tyr Gln Phe Asn Lys Lys Val Asn Arg Glu Lys Gln 
    450                 455                 460                 


Lys Leu Leu Pro Pro Val Pro Ala Lys Asp Ile Val Asn Pro Val Val 
465                 470                 475                 480 


Ile Arg Ser Leu Ser Gln Val Arg Lys Val Ile Asn Gly Ile Ile Lys 
                485                 490                 495     


Arg Tyr Gly Ser Pro Ser Ser Ile Tyr Ile Glu Leu Ala Arg Glu Met 
            500                 505                 510         


Gly Arg Gly Tyr Lys Glu Arg Lys Asp Leu Glu Lys Gln Tyr Asn Lys 
        515                 520                 525             


Asn Arg Gln Met Asn Glu Gly Ala Lys Lys His Ile Leu Glu Leu Arg 
    530                 535                 540                 


Pro Glu Phe Gly Asp Pro Arg Gly His Asp Ile Leu Lys Tyr Lys Leu 
545                 550                 555                 560 


Trp Gln Glu Gln Asp Cys Arg Cys Ala Tyr Ser Phe Lys Ser Ile Pro 
                565                 570                 575     


Ile Lys Gln Leu Phe Glu Ile Gly Tyr Ala Glu Val Asp His Ile Ile 
            580                 585                 590         


Pro Tyr Ser Arg Ser Phe Asp Asp Ser Asn Asn Asn Lys Val Leu Val 
        595                 600                 605             


Leu Ala Ala Glu Asn Gln Asn Lys Gln Asn Arg Thr Pro Tyr Glu Trp 
    610                 615                 620                 


Phe Gly His Asp Glu Thr Lys Trp Gln Glu Phe Thr Ser Leu Val Thr 
625                 630                 635                 640 


Thr Met Lys Val Ser Lys Lys Lys Lys Ser Leu Leu Leu Lys Lys Asn 
                645                 650                 655     


Phe Asp Glu Glu Gln Ala Glu Glu Phe Arg Ser Arg His Leu Asn Asp 
            660                 665                 670         


Thr Arg Tyr Ile Thr Arg Tyr Ile Lys Thr Phe Ile Glu Asp Asn Leu 
        675                 680                 685             


Gln Phe Arg Met Glu Glu Gly Lys Lys Gln Tyr Val His Thr Val Asn 
    690                 695                 700                 


Gly Ala Tyr Thr Ser Leu Met Arg Lys Arg Trp Gly Phe Asn Lys Asn 
705                 710                 715                 720 


Arg Gln Glu Asn Asp Leu His His Ala Leu Asp Ala Val Ile Val Ala 
                725                 730                 735     


Val Ser Leu Pro Phe Lys His Lys Val Ser Ala Tyr Phe Lys Arg Arg 
            740                 745                 750         


Glu Glu Tyr Ala His Asn Gln Leu Lys Arg Glu Gly Glu Phe Phe Pro 
        755                 760                 765             


Glu Pro Trp Pro Gly Phe Ile His Glu Leu Glu Ile Arg Met Met Gln 
    770                 775                 780                 


Asp Ser Glu Asn Ile Lys Leu Ala Met Glu Ser Leu Asn Leu Lys Thr 
785                 790                 795                 800 


Tyr Asp Glu Ser Phe Ile Lys Glu Leu Lys Pro Ile Phe Val Ser Arg 
                805                 810                 815     


Met Pro Lys Arg Ala Met Arg Gly Gln Ile His Glu Glu Thr Leu Lys 
            820                 825                 830         


Arg Val Arg Gly Arg Thr Asp Asp Gly Tyr Ile Arg Thr Val Lys Lys 
        835                 840                 845             


Val Lys Leu Asp Gln Ile Pro Phe Asp Lys Asn Gly Asp Phe Pro Met 
    850                 855                 860                 


Phe Gly Lys Glu Thr Asp Met Lys Thr Tyr Glu Ala Ile Lys Ser Arg 
865                 870                 875                 880 


Tyr Leu Glu Ser Gly Lys Asp Lys Lys Lys Ala Phe Asn Thr Pro Leu 
                885                 890                 895     


Tyr Lys Pro Ala Lys Asn Pro Glu Asn Ala Pro Ile Ile Arg Ser Ile 
            900                 905                 910         


Lys Ile Glu Glu Lys Ala Asn Arg Val Val Met Leu Asp Glu Lys Thr 
        915                 920                 925             


Ala Ala Asp Asn Ala Ser Ile Val Arg Thr Glu Val Phe Arg His Lys 
    930                 935                 940                 


Glu Thr Gly Asn Tyr Phe Leu Thr Pro Val Tyr Val Ala Asp Val Leu 
945                 950                 955                 960 


Ala Lys Lys Val Pro Asp Arg Leu Ile Thr Thr Lys Lys Pro Tyr Ser 
                965                 970                 975     


Glu Trp Arg Lys Ile Thr Asp Glu Tyr Glu Phe Leu Phe Ser Leu Tyr 
            980                 985                 990         


Pro Asn Asp Leu Ile Arg Val Lys  Met Pro Lys Glu Lys  Gly Thr Lys 
        995                 1000                 1005             


Thr His  Thr Gly Glu Lys His  Val Trp Gln Glu Asn  Phe Met Tyr 
    1010                 1015                 1020             


Phe Lys  Gly Val His Ser Gly  Ser Ala Gly Val Arg  Met Ile Asp 
    1025                 1030                 1035             


His Met  Asn Ser Phe Glu Asp  Glu Ile Gly Val Arg  Lys Leu Val 
    1040                 1045                 1050             


Val Phe  Glu Lys Tyr Gln Val  Asp Pro Leu Gly Arg  Met Asn Lys 
    1055                 1060                 1065             


Val His  Gly Glu Lys Arg His  Gly Ile 
    1070                 1075         


<210>  581
<211>  1077
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  581

Met Gln Gln Lys Pro Gln Arg Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ala Cys Val Trp Ala Asp Glu Asn Glu His Pro 
            20                  25                  30          


Thr Gly Leu Leu Asp Cys Gly Val Arg Thr Phe Glu Arg Ala Glu Val 
        35                  40                  45              


Pro Lys Thr Gly Asp Ser Leu Ala Leu Ala Arg Arg Gln Ala Arg Ser 
    50                  55                  60                  


Val Arg Arg Leu Ile Arg Arg Arg Ala His Arg Leu Leu Arg Leu Arg 
65                  70                  75                  80  


Arg Leu Leu Lys Arg Glu Gly Val Leu Leu Ser Ala Asp Phe Asn Glu 
                85                  90                  95      


Asn Gly Leu Val Arg Gly Leu Pro Ile Asp Ala Trp Ala Leu Arg Val 
            100                 105                 110         


Ala Gly Leu Asp Arg Lys Leu Ser Asn Lys Glu Trp Ala Ala Val Leu 
        115                 120                 125             


Leu His Leu Val Lys His Arg Gly Tyr Leu Ser Gln Arg Lys Asn Glu 
    130                 135                 140                 


Thr Gln Ser Lys Asp Lys Glu Leu Gly Arg Leu Leu Ala Gly Val Asn 
145                 150                 155                 160 


Gly Asn Arg Glu Ala Leu Gln Asn Arg Ser Asp Glu Tyr Arg Thr Ala 
                165                 170                 175     


Ala Glu Leu Ala Val Lys Lys Phe Ala Ala Glu Asn Asn Gly Ser Leu 
            180                 185                 190         


Arg Asn Lys Gly Gly Asp Tyr Ser His Thr Phe Asp Arg Lys Asp Leu 
        195                 200                 205             


Gln Arg Glu Leu His Leu Leu Phe Glu Arg Gln Arg Ala Leu Asp Asn 
    210                 215                 220                 


Pro Phe Thr Ser Ser Glu Met Glu Ala Ala Val Asp Glu Leu Leu Met 
225                 230                 235                 240 


Lys Gln Arg Ser Ala Leu Gln Gly Glu Ala Val Leu Lys Met Leu Gly 
                245                 250                 255     


Arg Cys Thr Phe Glu Pro Ser Glu Tyr Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Ser Ala Glu Arg Phe Val Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Gln Tyr Asn Gly Glu Glu Arg Ala Leu Ser Glu Ala Glu Arg Arg Leu 
    290                 295                 300                 


Leu Leu Asp Glu Pro Tyr Gln Lys Ala Lys Leu Thr Tyr Lys Gln Val 
305                 310                 315                 320 


Arg Thr Ile Leu Glu Leu Pro Glu Ser Ala Val Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly His Gly Glu Asp Gly Leu Lys Ala Glu Ser Gly Thr Leu Met 
            340                 345                 350         


Glu Met Lys Ala Tyr His Ala Val Arg Lys Ala Leu Lys Asp Ala Gly 
        355                 360                 365             


Leu Glu Gly Glu Trp Glu Asn Arg Ile Arg Asn Pro Glu Leu Leu Asp 
    370                 375                 380                 


Ala Ile Gly Thr Ala Phe Ser Leu Tyr Lys Thr Asp Glu Asp Ile Arg 
385                 390                 395                 400 


Ala Asn Leu Ala Ala Phe Arg Leu Ser Glu Asn Val Leu Glu Ala Leu 
                405                 410                 415     


Leu Ala Gly Leu Asn Phe Asp Lys Phe Val His Leu Ser Leu Lys Ala 
            420                 425                 430         


Leu Ala Lys Ile Leu Pro Leu Met Glu Gln Gly Met Arg Tyr Asp Glu 
        435                 440                 445             


Ala Cys Lys Glu Ala Tyr Gly Asp His Tyr Gly Ala Lys Glu Gln Glu 
    450                 455                 460                 


Asp His Arg Leu Leu Pro Pro Phe Val Ala Asp Asp Ile Arg Asn Pro 
465                 470                 475                 480 


Val Val Phe Arg Thr Leu Thr Gln Ala Arg Lys Ile Val Asn Glu Ile 
                485                 490                 495     


Val Arg Arg Tyr Gly Ser Pro Leu Arg Val His Ile Glu Thr Gly Arg 
            500                 505                 510         


Glu Val Gly Lys Ser Phe Ser Asp Arg Lys Glu Ile Glu Lys Arg Gln 
        515                 520                 525             


Glu Glu Asn Arg Lys Glu Arg Glu Arg Ala Ala Ala His Phe Lys Glu 
    530                 535                 540                 


Leu Phe Pro Asn Phe Ala Gly Glu Pro Lys Ala Gln Asp Ile Leu Lys 
545                 550                 555                 560 


Leu Arg Leu Tyr Glu Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys 
                565                 570                 575     


Glu Ile Asp Leu Arg Arg Leu Pro Glu Lys Gly Tyr Val Glu Ile Asp 
            580                 585                 590         


His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys 
        595                 600                 605             


Val Leu Val Leu Gly Lys Glu Asn Gln Asp Lys Arg Asn Arg Thr Pro 
    610                 615                 620                 


Tyr Glu Tyr Leu Asp Gly Lys Gly Asn Ser Gly Ala Trp Arg Ala Phe 
625                 630                 635                 640 


Ala Glu Arg Val Ser Gly Cys Arg Phe Pro Tyr Ala Lys Lys Gln Arg 
                645                 650                 655     


Leu Leu Thr Gln Lys Leu Asp Glu Lys Thr Glu Gln Gly Phe Leu Glu 
            660                 665                 670         


Arg Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe Leu Cys Gln Phe 
        675                 680                 685             


Ile Glu Glu Lys Met His Leu Glu Gly Ala Gly Lys Lys Arg Val Phe 
    690                 695                 700                 


Ala Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg Gly Phe Trp Gly Leu 
705                 710                 715                 720 


Lys Lys Val Arg Glu Glu Asn Asp Arg His His Ala Leu Asp Ala Val 
                725                 730                 735     


Val Val Ala Cys Ser Ser Val Ser Met Gln Gln Lys Ile Thr Arg His 
            740                 745                 750         


Phe Arg Tyr Arg Glu Glu Leu Glu His Ile Asp Arg Glu Thr Gly Glu 
        755                 760                 765             


Val Ile His Gly Phe Pro Gln Pro Trp Glu Phe Phe Arg Gln Glu Val 
    770                 775                 780                 


Met Thr Arg Val Phe Ser Asp Asp Pro Val Ser Glu Leu Ala Glu Lys 
785                 790                 795                 800 


Leu Asp Ser Arg Pro Glu Ala Val His Glu Phe Val Thr Pro Leu Phe 
                805                 810                 815     


Val Ser Arg Ala Pro His Arg Lys Val Thr Gly Gln Gly His Leu Glu 
            820                 825                 830         


Thr Ile Arg Ser Ala Lys Arg Leu Asp Glu Lys Ile Ser Val Val Lys 
        835                 840                 845             


Leu Pro Leu Thr Gln Leu Lys Thr Lys Asp Ile Glu Arg Ile Val Gly 
    850                 855                 860                 


Tyr Pro Asn Arg Glu Pro Ala Leu Tyr Asp Ala Leu Ala Ala Arg Leu 
865                 870                 875                 880 


Ala Ala Phe Lys Asp Asp Pro Ala Lys Ala Phe Ser Glu Pro Phe Tyr 
                885                 890                 895     


Lys Thr Gly Lys Asp Gly Lys Cys Gly Val Leu Val Lys Ala Val Arg 
            900                 905                 910         


Val Glu Asn Val Gln Lys Ser Gly Leu Leu Val Arg Lys Asp Glu Ser 
        915                 920                 925             


Gly Lys Met Gln Gly Val Ala Asp Asn Ala Thr Met Val Arg Val Asp 
    930                 935                 940                 


Val Phe Gly Lys Asn Gly Lys Asn Tyr Leu Val Pro Val Tyr Ala Trp 
945                 950                 955                 960 


Gln Val Glu Lys Gly Glu Leu Pro Asn Arg Ala Val Val Ala Tyr Ala 
                965                 970                 975     


Asp Glu Glu Asp Trp Asp Glu Met Asp Asp Ser Phe Glu Phe Lys Phe 
            980                 985                 990         


Ser Leu Tyr Pro Asn Asp Leu Val  Glu Val Val Thr Lys  Lys Asp Lys 
        995                 1000                 1005             


Phe Phe  Gly Tyr Tyr Ser Gly  Phe Asn Arg Ala Thr  Gly Ala Ile 
    1010                 1015                 1020             


Asn Ile  Lys Glu His Asp Leu  Ser Lys Ser Lys Val  Lys Asn Gly 
    1025                 1030                 1035             


Val Tyr  Glu Gly Ile Gly Val  Lys Thr Ala Gln Ser  Phe Gln Lys 
    1040                 1045                 1050             


Tyr Gln  Val Asp Thr Leu Gly  Lys Thr Ile Arg Leu  Cys Lys Pro 
    1055                 1060                 1065             


Glu Lys  Arg Met Gly Phe Lys  Lys Lys 
    1070                 1075         


<210>  582
<211>  1078
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  582

Met Arg Tyr Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Cys Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Gln Glu Lys Glu Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Lys Ala Glu Asn Pro Lys Asp Gly Lys Ser Leu Ala Ala 
        35                  40                  45              


Pro Arg Arg Asp Ala Arg Ser Thr Arg Arg Thr Leu Arg Arg Lys Lys 
    50                  55                  60                  


His Arg Met Gln Arg Ile Lys Val Leu Leu Val Lys His Gly Leu Leu 
65                  70                  75                  80  


Ser Lys Thr Glu Leu Asp His Leu Tyr Glu Ser Ala Thr Glu Val Asp 
                85                  90                  95      


Val Trp Asn Leu Arg Leu Asp Ala Leu Glu Arg Lys Leu Tyr Pro Lys 
            100                 105                 110         


Glu Phe Ala Arg Ile Leu Ile His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Glu Thr Thr Leu Ser Glu Asn Gly Gln Ile Leu Glu 
    130                 135                 140                 


Ser Ile Ser Glu Asn Gln Gln Ile Met Glu Lys Gln Asn Tyr Arg Thr 
145                 150                 155                 160 


Val Gly Glu Met Ile Leu Lys Asp Lys Lys Phe Glu Asn His Lys Arg 
                165                 170                 175     


Asn Lys Asn Gly Thr Tyr Val Gly Thr Val Thr Arg Gln Gln Leu Gln 
            180                 185                 190         


Asp Glu Ile Gln Met Ile Phe His Ala Gln Arg Leu Tyr Lys Asn Asp 
        195                 200                 205             


Tyr Ala Thr Glu Glu Phe Glu Ser Ser Tyr Leu Glu Ile Trp Ala Ser 
    210                 215                 220                 


Gln Arg Pro Tyr Ala Ser Lys Asp Gln Ile Glu Lys Met Ile Gly Tyr 
225                 230                 235                 240 


Cys Thr Leu Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Ser Trp Ser 
                245                 250                 255     


Phe Gln Tyr Phe Val Ala Leu Gln Thr Ile Asn Asn Leu Arg Leu Ile 
            260                 265                 270         


Ser Lys Asp Arg Ile Glu Glu Leu Ser Phe Glu Glu Lys Lys Gln Ile 
        275                 280                 285             


Met Asn Leu Ala Leu Glu Lys Ser Ile Val Lys Tyr Ile Asp Ile Arg 
    290                 295                 300                 


Lys Leu Leu Ser Val Pro Asn Glu Phe Gln Phe Asn Ser Leu Ser Tyr 
305                 310                 315                 320 


Ser Ala Asp Thr Val Asp Thr Ala Val Glu Ser Arg Lys Cys Ile Glu 
                325                 330                 335     


Leu Lys Glu Tyr His Ser Ile Asn Lys Val Tyr Lys Gln Ile Tyr Gly 
            340                 345                 350         


Glu Asn Ala Leu Asn Leu Leu Pro Lys Tyr Tyr Asp Thr Ile Ala Tyr 
        355                 360                 365             


Gly Leu Thr Ile Phe Lys Asp Asp Lys Asp Ile Leu Glu Tyr Leu Gln 
    370                 375                 380                 


Asn Lys Tyr Val Asn Ala Lys Gly Lys Pro Ile Ser Asn Phe Ala Asn 
385                 390                 395                 400 


Lys Thr Tyr Asp Asp Thr Phe Ile Gln Ala Leu Leu Asn Leu Asn Phe 
                405                 410                 415     


Thr Lys Met Gly His Leu Ser Phe Lys Ala Leu Lys Asn Ile Ile Pro 
            420                 425                 430         


Phe Leu Glu Glu Gly Leu Ser Tyr Asp Lys Ala Cys Glu Lys Ala Gly 
        435                 440                 445             


Tyr Asn Phe Lys Gly Thr Ser Tyr Thr Glu Gln Thr Lys Tyr Leu Pro 
    450                 455                 460                 


Val Ile Pro Gln Asn Thr Asn Pro Val Val His Arg Ala Leu Ser Gln 
465                 470                 475                 480 


Thr Arg Lys Val Ile Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Asn 
                485                 490                 495     


Ala Ile His Ile Glu Thr Ala Arg Glu Leu Ser Lys Thr Phe Gln Glu 
            500                 505                 510         


Arg Lys Glu Ile Asp Ser Met Tyr Gln Asp Asn Ser Lys Lys Asn Glu 
        515                 520                 525             


His Ala Ile His Lys Leu Lys Glu Leu Gly Asn Ile Asn Pro Ser Gly 
    530                 535                 540                 


Ile Asn Ile Val Lys Phe Lys Leu Trp Asn Glu Gln Asp Gly Arg Cys 
545                 550                 555                 560 


Met Tyr Ser Gly Lys Tyr Ile Glu Pro His Arg Leu Phe Glu Glu Gly 
                565                 570                 575     


Tyr Thr Glu Val Asp His Ile Leu Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Asn Asn Lys Thr Leu Thr Leu Gly Thr Glu Asn Gln Arg Lys 
        595                 600                 605             


Gly Asn Lys Ile Pro Tyr Glu Tyr Met Gly Asn Thr Ser Ile Trp Asn 
    610                 615                 620                 


Asp Phe Glu Thr Arg Val Gln Ser Asn Lys Lys Ile Asn Lys Lys Lys 
625                 630                 635                 640 


Gln Gln Lys Leu Leu Leu Gln His Phe Ser Tyr Ala Arg Glu Gln Glu 
                645                 650                 655     


Phe Ile Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ala Thr Ile Tyr Leu 
            660                 665                 670         


Ser Ser Leu Ile Gln Gln His Leu Ile Phe Ser Glu Ser Ser Arg Lys 
        675                 680                 685             


Lys Lys Val His Thr Val Ser Gly Ile Ile Thr Ser His Leu Arg Ser 
    690                 695                 700                 


Arg Trp Gly Phe Asn Lys Asp Arg Lys Glu Gly His Ile His His Ala 
705                 710                 715                 720 


Leu Asp Ala Val Ile Val Ala Val Thr Ser Asp His Met Ile Gln Arg 
                725                 730                 735     


Val Thr Lys Tyr Tyr Lys Leu Lys Glu Leu Asn Arg Asn Leu Gln Ala 
            740                 745                 750         


Lys Arg Met Pro Phe Pro Glu Pro Trp Glu Gly Phe Arg Leu Glu Leu 
        755                 760                 765             


Glu Ala Arg Ile Ser Pro Asn Thr Gln Gln Tyr Leu Lys Gly Leu Arg 
    770                 775                 780                 


Phe Lys Asn Tyr Ala Asp Val Asn Leu Ser Glu Ile Lys Ser Ile Phe 
785                 790                 795                 800 


Val Ser Arg Met Pro Lys His Ser Ile Thr Gly Glu Leu His Gln Glu 
                805                 810                 815     


Thr Ile Arg Lys Phe Ile Gly Tyr Asn Glu Lys Gly Lys Val Leu Thr 
            820                 825                 830         


Ala Ile Lys Thr Lys Leu Glu Asp Ile Pro Phe Asp Ala Asn Gly Asp 
        835                 840                 845             


Phe Pro Met Tyr Gly Lys Glu Ser Asp Leu Tyr Thr Tyr Asn Ala Ile 
    850                 855                 860                 


Lys Glu Arg Tyr Leu Ser His Lys Lys Asp Lys Arg Lys Ser Phe Gln 
865                 870                 875                 880 


Glu Pro Leu Tyr Lys Pro Thr Lys Ser Gly Gly Ile Gly Pro Leu Ile 
                885                 890                 895     


Lys Ser Ile Lys Ile Met Asp Thr Arg Thr Ile Val Asn Pro Val Asn 
            900                 905                 910         


Gln Gly Lys Gly Val Val Tyr Asn Ser Lys Ile Ala Arg Thr Asp Val 
        915                 920                 925             


Phe Lys Lys Glu Glu Lys Tyr Tyr Leu Ile Pro Ile Tyr Thr Ile Asp 
    930                 935                 940                 


Leu Met Lys Asn Ile Leu Pro Gln Lys Ala Ile Thr Ala Gly Lys Gly 
945                 950                 955                 960 


Tyr Glu Asp Trp Thr Thr Ile Asp Pro Ser Phe Thr Phe Leu Phe Ser 
                965                 970                 975     


Leu Phe Pro Asn Asp Leu Ile Gln Ile Val Pro Ser Lys Asn Lys Thr 
            980                 985                 990         


Ile Lys Ala Arg Thr Thr Val Ser  Lys Lys Glu Val Leu  Leu Pro Ser 
        995                 1000                 1005             


Leu Thr  Gly Tyr Phe Lys Gly  Val His Ser Gly Thr  Ala Gly Ile 
    1010                 1015                 1020             


Thr Val  Glu Thr His Asp Gly  Ser Val Ile Ala Asn  Val Gly Ser 
    1025                 1030                 1035             


Lys Gln  Leu Leu Leu Ile Glu  Lys Tyr Gln Val Asp  Val Leu Gly 
    1040                 1045                 1050             


Asn Tyr  Thr Lys Ile Lys Glu  Glu Lys Arg Ile Gly  Leu Thr Thr 
    1055                 1060                 1065             


Tyr Asn  Asp Asn Lys Lys Ile  Ala Ala Cys 
    1070                 1075             


<210>  583
<211>  1078
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  583

Met Lys Gln Gln Pro Gln Arg Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ala Leu Ile Leu Val Asp Glu Glu Gly Asn Pro 
            20                  25                  30          


Ile Gly Leu Leu Asp Cys Gly Val Arg Thr Phe Glu Arg Ala Glu Val 
        35                  40                  45              


Pro Lys Thr Gly Asp Ser Leu Ala Leu Ala Arg Arg Gln Ala Arg Ser 
    50                  55                  60                  


Val Arg Arg Leu Ile Arg Arg Arg Ala His Arg Leu Leu Arg Leu Arg 
65                  70                  75                  80  


Arg Leu Leu Lys Arg Glu Gly Val Leu Ser Ala Ala Asp Phe Asn Ser 
                85                  90                  95      


Asp Gly Leu Pro Asn Leu Pro Ile Asp Thr Trp Gln Leu Arg Val Ala 
            100                 105                 110         


Gly Leu Glu Arg Lys Leu Thr Asn Gln Glu Trp Ala Ala Val Leu Leu 
        115                 120                 125             


His Leu Val Lys His Arg Gly Tyr Leu Ser Gln Arg Lys Ser Glu Thr 
    130                 135                 140                 


Gln Thr Glu Asn Lys Glu Leu Gly Cys Leu Leu Ala Ser Val Ala Lys 
145                 150                 155                 160 


Asn Ser Lys Leu Leu Ser Glu Asn Ala Asn Gln Tyr Arg Thr Pro Ala 
                165                 170                 175     


Glu Leu Ala Val Arg Leu Phe Ala Glu Gln Ser Gly His Leu Arg Asn 
            180                 185                 190         


Lys Gly Gly Asp Tyr Thr His Thr Phe Asn Arg Leu Asp Leu Gln Lys 
        195                 200                 205             


Glu Leu His Leu Leu Phe Glu Gln Gln Arg Thr Phe Gly Asn Pro Phe 
    210                 215                 220                 


Ala Ser Ala Glu Leu Gln Ser Lys Val Asp Gly Leu Leu Met Met Gln 
225                 230                 235                 240 


Arg Ser Ala Leu Gln Gly Glu Ala Val Leu Lys Met Leu Gly Lys Cys 
                245                 250                 255     


Thr Phe Glu Pro Ser Glu Tyr Lys Ala Ala Lys His Thr Tyr Ser Ala 
            260                 265                 270         


Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile Leu Glu 
        275                 280                 285             


Asn Gly Thr Glu Arg Ala Leu Asn Glu Ala Glu Arg Lys Leu Leu Leu 
    290                 295                 300                 


Asp Glu Pro Tyr Lys Lys Ala Lys Leu Thr Tyr Ser Gln Ala Arg Lys 
305                 310                 315                 320 


Leu Leu Asp Met Ser His Asp Ala Thr Phe Lys Gly Leu Arg Tyr Gly 
                325                 330                 335     


Lys Asp Ser Glu Gly Leu Asn Ala Glu Asn Gly Ala Leu Met Glu Met 
            340                 345                 350         


Lys Ala Tyr His Lys Ile Arg Thr Val Leu Asp Lys Ala Gly Leu Lys 
        355                 360                 365             


Gly Glu Trp Glu Asn Arg Ile Arg Asn Pro Glu Ile Leu Asp Lys Ile 
    370                 375                 380                 


Gly Thr Ala Phe Ser Leu Tyr Lys Thr Asp Glu Asp Ile Gln Ala Ala 
385                 390                 395                 400 


Leu Ala Asp Leu Arg Leu Pro Glu Asn Val Leu Asn Ala Leu Leu Ala 
                405                 410                 415     


Gly Leu Asn Phe Asp Lys Phe Ile Gln Leu Ser Leu Lys Ala Leu Ser 
            420                 425                 430         


Asn Ile Met Pro Leu Met Glu Arg Gly Ile Arg Tyr Asp Glu Ala Cys 
        435                 440                 445             


Lys Glu Val Tyr Gly Asp His Arg Ala Leu Ala Glu Ser Lys Asn Gln 
    450                 455                 460                 


Met Leu Leu Pro Pro Ile Pro Ala Asn Glu Ile Arg Asn Pro Val Val 
465                 470                 475                 480 


Leu Arg Thr Leu Thr Gln Thr Arg Lys Val Ile Asn Ala Ile Ile Arg 
                485                 490                 495     


Arg Tyr Gly Ser Pro Gln Arg Val His Ile Glu Thr Ala Arg Glu Leu 
            500                 505                 510         


Gly Lys Ser Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu 
        515                 520                 525             


Asn Arg Lys Glu Arg Glu Arg Ala Ala Glu His Phe Lys Glu Leu Phe 
    530                 535                 540                 


Pro His Phe Ala Gly Glu Pro Lys Gly Gly Asp Ile Leu Lys Leu Arg 
545                 550                 555                 560 


Leu Tyr Glu Gln Gln Gln Gly Lys Cys Leu Tyr Ser Ser Ile Pro Ile 
                565                 570                 575     


Asp Leu Gly Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala 
            580                 585                 590         


Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu 
        595                 600                 605             


Val Leu Gly Ser Glu Asn Gln Lys Lys Arg Asn Gln Thr Pro Tyr Glu 
    610                 615                 620                 


Tyr Leu Asp Gly Ala Ser Asn Ser Gln Arg Trp Arg Glu Phe Gln Ala 
625                 630                 635                 640 


Arg Leu Gln Gly Cys Arg Phe Ser Tyr Ala Lys Lys Gln Arg Ile Gln 
                645                 650                 655     


Thr Ala Lys Leu Asp Ser Lys Thr Glu Gln Gly Phe Leu Glu Arg Asn 
            660                 665                 670         


Leu Asn Asp Thr Arg Tyr Ile Ala Arg Phe Leu Cys Gly Phe Ile Glu 
        675                 680                 685             


Glu Asn Leu His Leu Gln Gly Glu Gly Lys Arg Arg Val Phe Ala Ser 
    690                 695                 700                 


Asn Gly Gln Ile Thr Ala Leu Leu Arg Gly Leu Trp Gly Leu Lys Lys 
705                 710                 715                 720 


Val Arg Glu Glu Asn Asp Arg His His Ala Leu Asp Ala Ile Val Val 
                725                 730                 735     


Ala Cys Ser Thr Val Ser Met Gln Gln Lys Ile Thr Lys Ala Met Gln 
            740                 745                 750         


Arg Arg Glu Thr Leu Glu Thr Val Asp Thr Glu Thr Gly Glu Val Lys 
        755                 760                 765             


Thr Arg Ile Pro Gln Pro Trp Asp Phe Phe Arg Gln Glu Val Met Met 
    770                 775                 780                 


Arg Val Phe Ser Asp Thr Pro His Glu Glu Leu Ala Glu Lys Leu Ser 
785                 790                 795                 800 


Gly Arg Pro Glu Ala Leu His Asp Phe Val Ser Pro Leu Phe Val Ser 
                805                 810                 815     


Arg Ala Pro Asn Arg Lys Met Thr Gly Gln Gly His Leu Glu Thr Val 
            820                 825                 830         


Lys Ser Ala Lys Arg Leu Ser Glu Asn Ile Ser Val Val Arg Lys Pro 
        835                 840                 845             


Leu Thr Ala Leu Lys Leu Lys Asp Ile Glu Asn Ile Val Gly Tyr Pro 
    850                 855                 860                 


Asp Arg Glu Pro Ala Leu Tyr Gln Ala Leu Arg Glu Arg Leu Gln Ala 
865                 870                 875                 880 


Tyr Lys Asp Asp Pro Val Lys Ala Phe Ala Glu Pro Phe Tyr Lys Pro 
                885                 890                 895     


Ala Lys Asn Gly Gly Gln Gly Ser Leu Val Lys Ala Val Arg Val Lys 
            900                 905                 910         


Asp Val Gln Lys Thr Gly Val Met Val Arg Lys Asp Arg Gly Gly Ile 
        915                 920                 925             


Ala Arg Gly Ile Ala Asp Asn Ala Thr Met Val Arg Val Asp Val Phe 
    930                 935                 940                 


Gly Lys Lys Gly Lys Asn Tyr Leu Val Pro Val Tyr Ala Trp Gln Val 
945                 950                 955                 960 


Ala Lys Gly Ile Leu Pro Asn Arg Ala Val Val Ala Tyr Ala Asp Glu 
                965                 970                 975     


Ala Asp Trp Asp Glu Met Asp Asp Ser Tyr Glu Phe Lys Phe Thr Leu 
            980                 985                 990         


Tyr Pro Asn Asp Leu Val Glu Ile  Ile Ser Lys Lys Gly  Arg Ile Phe 
        995                 1000                 1005             


Gly Tyr  Tyr Ser Gly Leu Asn  Arg Ala Thr Gly Ala  Ile Asp Ile 
    1010                 1015                 1020             


Lys Glu  His Asp Leu Ser Lys  Glu Lys Ala Lys Asn  Gly Ile His 
    1025                 1030                 1035             


Gln Ser  Val Gly Val Lys Thr  Val Leu Ser Phe Gln  Lys Tyr Gln 
    1040                 1045                 1050             


Val Asp  Pro Leu Gly Lys Glu  Ile Cys Leu Cys Lys  Val Glu Lys 
    1055                 1060                 1065             


His Pro  Glu Leu Asn Ser Lys  Lys Lys Lys 
    1070                 1075             


<210>  584
<211>  1078
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  584

Met Gly Tyr Leu Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Tyr 
1               5                   10                  15      


Ala Val Leu Gln Thr Asp Phe Lys Gly Asn Pro Tyr Lys Ile Leu Leu 
            20                  25                  30          


Met Asn Thr Val Ile Phe Pro Val Ala Glu Ala Pro Lys Thr Gly Glu 
        35                  40                  45              


Ser Leu Ala Ala Pro Val Arg Gln Lys Arg Ser Ser Arg Arg Thr Asn 
    50                  55                  60                  


Arg Arg Thr Lys Phe Arg Lys Leu Arg Val Gln Lys Leu Phe Val Arg 
65                  70                  75                  80  


Phe Gly Leu Leu Glu Ser Ala Asp Val Ala Thr Phe Leu Asn Gln Lys 
                85                  90                  95      


Val Pro His Gln Asp Ile Trp Gln Leu Arg Lys Asp Ala Leu Asn Arg 
            100                 105                 110         


Lys Leu Ser Asn Arg Glu Leu Phe Glu Ile Leu Tyr Phe Phe Val Gly 
        115                 120                 125             


His Arg Gly Phe Lys Ser Asn Arg Arg Ser Glu Val Thr Gly Lys Gln 
    130                 135                 140                 


Pro Lys Asp Asn Gln Val Val Leu Thr Ser Ile His Asp Arg Gly Asn 
145                 150                 155                 160 


Thr Leu Asn Asp Phe Asp Ser Leu Gly Glu Met Met Gln Glu Leu Pro 
                165                 170                 175     


Glu Phe Lys Thr Ile Lys His Asn Lys Ala Tyr Arg Pro Asp Asn Thr 
            180                 185                 190         


Ile Tyr Pro Leu Arg Ala Trp Ile Glu His Glu Leu Ser Ala Ile Phe 
        195                 200                 205             


Ser Val Gln Gln Lys Asn Asn Arg Gln Ile Thr Asp Asn Phe Ile Thr 
    210                 215                 220                 


Gln Tyr Leu Asp Ile Phe Arg Ser Gln Arg Asp Phe Asp Gln Gly Pro 
225                 230                 235                 240 


Ala Ala Pro Ser Arg Phe Gly Gly Asn Met Ile Glu Arg Met Val Gly 
                245                 250                 255     


Pro Asp Ser Leu Asp Ala His Glu Lys Arg Ala Ala Lys Leu Thr Asn 
            260                 265                 270         


Thr Phe Phe Arg Phe Asn Phe Leu Ser Thr Leu Asn Asn Leu Lys Ile 
        275                 280                 285             


Gln Leu Thr Leu Gly Ala Glu Phe His Pro Leu Thr Thr Pro Gln Arg 
    290                 295                 300                 


Glu Ile Val Leu Gln Ala Ala Thr Leu Lys Ser Leu Asn Tyr Lys Gln 
305                 310                 315                 320 


Ile Arg His Lys Leu Gln Leu Pro Asp Ser Ala Arg Phe Asn Leu Val 
                325                 330                 335     


Pro Tyr Gln Ser Asn Glu Asp Pro Ile Lys Thr Glu Ser Lys His Tyr 
            340                 345                 350         


Leu Phe Lys Arg Lys Ala Leu Thr Gln Ile Glu Ala Val Thr Asp Asn 
        355                 360                 365             


Pro Gln Leu Ile Asp Lys Ile Gly Thr Ile Leu Ala Tyr Phe Lys Gly 
    370                 375                 380                 


Asp Lys Thr Arg Gln Glu Lys Leu Ser Ala Leu Asn Leu Ser Arg Ala 
385                 390                 395                 400 


Glu Ile Lys Gln Leu Leu Pro Ile Asn Gln Pro Gly Phe Gly Asn Leu 
                405                 410                 415     


Ser Leu Lys Thr Met His Lys Ile Leu Pro Tyr Leu Glu Gln Gly His 
            420                 425                 430         


Leu Tyr His Glu Ala Ala Gln Leu Ala Gly Tyr Asp Phe Gln Asn Asn 
        435                 440                 445             


Leu Val Asp Arg Gln Tyr Leu Arg Asp Asn Leu Lys Asn Pro Val Val 
    450                 455                 460                 


Asn Arg Ala Ile Gly Lys Thr Ile Lys Val Val Asn Ala Ile Ile Lys 
465                 470                 475                 480 


Lys Tyr Gly Asp Pro Asp Ala Ile His Ile Glu Leu Thr Arg Glu Ile 
                485                 490                 495     


Lys His Ser His Glu Glu Arg Gln Lys Ile Ala Arg Gln Met Asn Thr 
            500                 505                 510         


Asn Met Asp Gln Asn Asp Lys Val Ala Glu His Leu Arg Glu Asn Gly 
        515                 520                 525             


Ile Pro Val Asn Gly Gln Asn Ile Ile Lys Ser Lys Leu Phe Tyr Glu 
    530                 535                 540                 


Gln Lys Gly Ile Asp Leu Tyr Ser Gly Gln Gln Ile Asn Glu Gln Asp 
545                 550                 555                 560 


Leu Tyr Thr Glu Asp His Tyr Gln Val Asp His Ile Ile Pro Tyr Ser 
                565                 570                 575     


Ile Ser Phe Asn Asp Ser Phe Thr Asn Lys Thr Val Thr Arg Thr Ser 
            580                 585                 590         


Asn Asn Gln Asn Lys Ala Asn Arg Leu Pro Leu Glu Tyr Leu Ser Glu 
        595                 600                 605             


Glu Lys Gln Asp Ala Phe Arg Ala Arg Val Met Ser Asn Ile Ser Asn 
    610                 615                 620                 


Tyr Arg Lys Arg Gln Asn Leu Leu Lys Glu Gly Ile Thr Glu Asp Asn 
625                 630                 635                 640 


Arg Gln Gln Trp Lys Thr Arg Asn Ile Asn Asp Thr Gly Tyr Ile Asn 
                645                 650                 655     


Arg Leu Leu Ser Gln Tyr Leu Arg Asn Asn Val Gln Phe Thr Lys Lys 
            660                 665                 670         


Ser His Asn Pro Val Phe Thr Val Asn Gly Ala Thr Thr Ala Tyr Ile 
        675                 680                 685             


Arg Lys Arg Tyr Gly Ile Ile Lys Asn Arg Gln Ala Thr Asp Leu His 
    690                 695                 700                 


His Ala Val Asp Ala Val Ile Ile Gly Cys Ile Ser Asn Thr Phe Val 
705                 710                 715                 720 


Ser Arg Leu Ala Lys Tyr Ser Glu Ala Gln Glu Ala Arg Tyr His Lys 
                725                 730                 735     


Gln Leu Trp Gln Ile Thr Gln Tyr Arg Gln Glu His Lys Asp Leu Ser 
            740                 745                 750         


Thr Ile Glu Tyr Gln Glu Leu Ile Lys Gln Phe Pro Leu Pro Trp Pro 
        755                 760                 765             


Asp Phe Arg His Glu Leu Met Ala Arg Leu Ser Thr Asp Pro Ala Lys 
    770                 775                 780                 


Met Met Ala His His Thr Trp Glu His Tyr Thr Asp Glu Glu Ile Ser 
785                 790                 795                 800 


Gln Leu Lys Pro Ala Phe Val Ile Arg Ser Ala Asn His Lys Val Thr 
                805                 810                 815     


Gly Ser Ala His Asp Asp Thr Ile Tyr Ser Lys Lys Leu Tyr Asp Gln 
            820                 825                 830         


Arg Gly Val Gly Leu Leu Arg Thr Ser Leu Gln Asn Leu Lys Tyr Asp 
        835                 840                 845             


Ser Lys Lys Asp Gly Ile Lys Asn Tyr Glu Arg Leu Lys Asp Gly Ser 
    850                 855                 860                 


Asn Ser Leu Val Tyr Asp Ser Leu Leu Arg Ala Leu Lys Glu His Asp 
865                 870                 875                 880 


Gly Asn Ala Lys Lys Ala Phe Pro Asp Asn Gln Leu Leu Ile Ser Thr 
                885                 890                 895     


Gly Asn His Gln Gln Ile Ile Thr His Val Lys Thr Val Lys Lys Ile 
            900                 905                 910         


Thr His Pro Ile Glu Val Asn Arg Asp Thr Ala Ile Ala Ile Gly Gly 
        915                 920                 925             


Arg Met Ile Arg Val Asp Ile Phe Lys Gln Ala Lys Ala Gln Lys Leu 
    930                 935                 940                 


Ile Gly Ile Pro Val Tyr Val Asn Asp Thr Val Lys Pro Thr Leu Pro 
945                 950                 955                 960 


Asn Arg Ala Cys Thr Ser Gly Lys Arg Tyr Ser Asp Trp Tyr Gln Leu 
                965                 970                 975     


Thr Arg Gln Asp Gln Phe Leu Cys Ser Leu Val Ser Asn Asp Leu Val 
            980                 985                 990         


Leu Val Glu Thr Ala Ser Pro Leu  Lys Val Thr Asn Asn  Ile Thr Asn 
        995                 1000                 1005             


Glu Glu  Val Gln Thr Thr Gln  Leu Leu Cys Tyr Phe  Ser Ser Phe 
    1010                 1015                 1020             


Asn Ile  Ala Asn Ala Gln Ile  Asn Phe His Ser Ile  Asn Asn Asp 
    1025                 1030                 1035             


Ser Ser  Ile Asn Pro Ile Val  Leu Asn Lys Val Thr  Tyr Leu Thr 
    1040                 1045                 1050             


Lys Tyr  Gln Leu Asp Tyr Leu  Gly Asn Tyr Phe Pro  Val Lys His 
    1055                 1060                 1065             


Glu Ser  Arg Lys Thr Phe Asn  Arg Lys Arg 
    1070                 1075             


<210>  585
<211>  1078
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  585

Met His Thr Arg Leu Ala Leu Asp Ile Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ile Leu Tyr Glu Leu Val Asn Gly Gln Pro Ala Ser Ile Leu Asp Gly 
            20                  25                  30          


Gly Thr Arg Ile Phe Ser Asp Gly Arg Glu Ala Lys Thr Gly His Ser 
        35                  40                  45              


Leu Ala Val Gly Arg Arg Thr Ala Arg Gly Met Arg Arg Arg Arg Asp 
    50                  55                  60                  


Arg Tyr Leu Arg Arg Arg Ala Ala Leu Met Lys Arg Leu Ala Ala Ile 
65                  70                  75                  80  


Gly Leu Met Pro Glu Ser Pro Glu Ala Ala Ala Gln Leu Ala Thr Leu 
                85                  90                  95      


Asp Pro Tyr Glu Leu Arg Ala Arg Gly Leu Asp His Pro Leu Pro Pro 
            100                 105                 110         


Glu His Leu Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe 
        115                 120                 125             


Lys Ser Asn Arg Arg Thr Asp Lys Gly Asn Asn Glu Gly Gly Met Ile 
    130                 135                 140                 


Ala Asp Ala Ser Ala Arg Leu Glu Gln Ala Met Met Ala Ala Gly Ala 
145                 150                 155                 160 


Arg Ser Tyr Gly Glu Phe Leu His Lys Arg Arg Ala Glu Ala Thr Ala 
                165                 170                 175     


Pro Arg Ala Thr Pro Ala Val Arg Thr Arg Ile Asn Pro His Leu Thr 
            180                 185                 190         


Gly Glu Asp Gly Lys Glu Gln Pro Gly Tyr Asp Phe Tyr Pro Asp Arg 
        195                 200                 205             


Lys His Leu Glu Asp Glu Phe His Arg Leu Trp Glu Ala Gln Ala Arg 
    210                 215                 220                 


His Asn Pro Asp Leu Thr Pro Ala Met Gly Asp Leu Leu Phe Gln Thr 
225                 230                 235                 240 


Ile Phe His Gln Arg Pro Leu Lys Ala Pro Ala Val Gly Arg Cys Leu 
                245                 250                 255     


Phe Leu Asp Glu Pro Arg Leu Pro Lys Ala His Pro Leu Thr Gln Gln 
            260                 265                 270         


Arg Thr Leu Tyr Glu Thr Val Asn Met Leu Arg Ile Ala Pro Pro Gly 
        275                 280                 285             


Gln Pro Lys Arg Arg Leu Ser Arg Val Gln Arg Asp Gln Ile Val His 
    290                 295                 300                 


Ala Leu Asn Asn Arg Lys Ile Leu Ala Ser Pro Gly Lys Asn Val Leu 
305                 310                 315                 320 


Thr Leu Ala Ala Ile Gly Lys Leu Leu Lys Leu Arg Pro Gly Glu Ala 
                325                 330                 335     


Phe Ser Leu Asp Thr Ala Asn Arg Asp Ala Ile Ala Cys Asp Pro Val 
            340                 345                 350         


Arg Met Ser Leu Ala Tyr Glu Gly Arg Phe Gly Pro Gln Trp Ser Ala 
        355                 360                 365             


Leu Ser Val Glu Ser Gln Trp Glu Leu Ile Gln Arg Leu Arg Asp Asp 
    370                 375                 380                 


Gln Asp Gly Ala Ala Leu Val Asp Trp Ile Met Asp Arg Tyr Gly Phe 
385                 390                 395                 400 


Asp Arg Asp Arg Ala Arg Ala Ile Ala Asp Ala Pro Leu Pro Glu Gly 
                405                 410                 415     


Phe Gly Arg Leu Gly Glu Thr Ala Thr Arg Ala Val Leu Ala Val Leu 
            420                 425                 430         


Ile Gly Gly Thr Thr Glu Gln Glu Val Leu Thr Tyr Ser Glu Ala Val 
        435                 440                 445             


Ala Ala Cys Gly Trp Asp His Ser Arg Leu Gly Thr Gly Glu Val Leu 
    450                 455                 460                 


Asp Arg Leu Pro Tyr Tyr Gly Gln Val Leu Asp Arg His Val Leu Pro 
465                 470                 475                 480 


Gly Thr Gly Arg Pro Glu Asp Asp Glu Ile Thr Arg Phe Gly Arg Ile 
                485                 490                 495     


Thr Asn Pro Thr Val His Ile Gly Leu Gly Gln Val Lys Arg Leu Val 
            500                 505                 510         


Asn Lys Val Ile Asp Ala Trp Gly Arg Pro His Glu Met Val Val Glu 
        515                 520                 525             


Leu Ala Arg Asp Leu Lys Met Ser Glu Asp Gln Lys Arg Asn Leu Ala 
    530                 535                 540                 


Lys Val Asn Ala Gln Asn Gln Arg Ala Ala Glu Met Arg Ser Ala Lys 
545                 550                 555                 560 


Leu Ile Gly Asp Leu Lys Ile Glu Asp Asn Gly Arg Asn Arg Gly Ile 
                565                 570                 575     


Leu Arg Val Trp Glu Glu Ser Ala Ala Asp Val Leu His Arg Cys Cys 
            580                 585                 590         


Pro Tyr Thr Gly Ala Gln Ile Ser Ala Ser Met Leu Phe Asp Gly Ser 
        595                 600                 605             


Cys Asp Ile Asp His Ile Leu Pro Tyr Ser Arg Thr Leu Asp Asp Gly 
    610                 615                 620                 


Phe Asn Asn Arg Val Ile Cys Leu Lys Ser Ala Asn Arg Glu Lys Arg 
625                 630                 635                 640 


Asn Leu Thr Pro Trp Glu Ala Trp Gly Asn Thr Pro Lys Trp Ala Ala 
                645                 650                 655     


Ile Glu Ala Asn Leu Lys Asn Leu Pro Pro Ala Lys Ala Trp Arg Phe 
            660                 665                 670         


Ala Pro Asp Ala Met Leu Arg Tyr Glu Gly Glu Arg Asp Phe Thr Ala 
        675                 680                 685             


Arg Ala Leu Val Asp Thr Gln Tyr Leu Ser Arg Leu Ala Arg Glu Tyr 
    690                 695                 700                 


Leu Glu Cys Leu Tyr Ala Glu Asp Ile Asp Pro Gly Pro His Ser Gly 
705                 710                 715                 720 


Lys Lys Pro Arg Arg Pro Ile Trp Val Val Thr Gly Lys Leu Thr Glu 
                725                 730                 735     


Leu Leu Arg Arg His Trp Gly Leu Asn Ser Leu Leu Pro Asp Ala His 
            740                 745                 750         


Arg Gly Thr Ala Lys Ala Lys Asn Arg Leu Asp His Arg His His Met 
        755                 760                 765             


Ile Asp Ala Ala Val Val Gly Ala Thr Asp His Ala Thr Val Gln Arg 
    770                 775                 780                 


Ile Ala Asn Met Ala Lys Gly Asn Val Glu Asn Gly Leu Glu Asp Leu 
785                 790                 795                 800 


Ala Gly Thr Ile Leu Pro Pro Trp Pro Gly Leu Arg Asp Asp Leu Lys 
                805                 810                 815     


Ala Arg Leu Glu Arg Thr Ile Val Ser His Arg Gly Asn His Gly Ala 
            820                 825                 830         


Ile Asp Pro Ala Ala Arg Lys Gln Gly Arg Asp Thr Thr Ser Gly Gln 
        835                 840                 845             


Leu His Asn Asp Thr Ala Tyr Gly Leu Thr Gly Glu Thr Leu Lys Gly 
    850                 855                 860                 


Val Pro Leu Val Val Asn Arg Lys Pro Ile Asp Met Leu Thr Pro Ala 
865                 870                 875                 880 


Met Ile Asp Lys Ile Arg Asp Pro His Leu Ser Arg Trp Leu Thr Ala 
                885                 890                 895     


Val Thr Lys Gly Lys Glu Gly Lys Asp Phe Lys Ala Ala Val Glu Ala 
            900                 905                 910         


Phe Arg Ala His Pro Gly Pro Tyr Arg Gly Ile Arg Arg Val Arg Leu 
        915                 920                 925             


Ile Glu Pro Val Glu Val Ile Arg Ile Lys Asp Arg Asn Gly Arg Pro 
    930                 935                 940                 


Tyr Lys Gly Tyr Lys Gly Asp Ser Asn Gln Cys Tyr Glu Val Trp Cys 
945                 950                 955                 960 


Leu Pro Asp Arg Thr Leu Thr His His Val Val Ser Thr Phe Ala Ala 
                965                 970                 975     


His Gln Gly Gln Thr Gly Lys Pro His Pro Ala Ala Lys Arg Leu Ala 
            980                 985                 990         


Arg Leu Tyr Lys Gly Asp Met Val  Arg Leu Asp Arg Ser  Lys Phe Gly 
        995                 1000                 1005             


Ser Val  Ile Ala Thr Val Glu  Lys Phe Asp Arg Asn  Gly Gln Leu 
    1010                 1015                 1020             


Glu Leu  Val Pro His Asn Glu  Gly Asn Ala Asp Gln  Arg Tyr Arg 
    1025                 1030                 1035             


Lys Asp  Lys Ser Asp Ser Val  Phe Ile Arg Leu Arg  Ala Ala Thr 
    1040                 1045                 1050             


Phe Ile  Ala Ser Gly Ala Arg  Arg Val Ile Val Asp  Glu Met Gly 
    1055                 1060                 1065             


Arg Ile  Arg Asp Pro Gly Pro  Gly Lys His 
    1070                 1075             


<210>  586
<211>  1079
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  586

Met Lys Tyr Gly Ile Gly Leu Asp Cys Gly Ile Asn Ser Val Gly Tyr 
1               5                   10                  15      


Cys Ile Met Glu Leu Asn Ser Asp Asp Glu Pro Lys Arg Ile Val Arg 
            20                  25                  30          


Leu Asn Ser Arg Ile Phe Thr Glu Ala Glu Asn Pro Lys Asp Gly Ser 
        35                  40                  45              


Ser Leu Ala Ala Pro Arg Arg Glu Ala Arg Gly Leu Arg Arg Arg Ile 
    50                  55                  60                  


Arg Arg His Lys His Arg Leu Glu Arg Ile Arg Ala Leu Leu Val Arg 
65                  70                  75                  80  


Glu Asn Ile Val Thr Gln Ala Glu Leu Asp Asn Met Phe Ser Gly Thr 
                85                  90                  95      


Leu Ser Asp Ile Tyr Glu Leu Arg Thr Ala Ala Leu Asp Ala Pro Leu 
            100                 105                 110         


Asn Asn Ile Glu Phe Ala Arg Val Leu Ile Asn Leu Ala Gln Arg Arg 
        115                 120                 125             


Gly Phe Lys Ser Asn Arg Lys Ser Glu Lys Ser Ser Ser Asp Lys Glu 
    130                 135                 140                 


Thr Gly Lys Leu Leu Ser Ala Ile Lys Lys Asn Lys Glu Ser Ile Glu 
145                 150                 155                 160 


Ala Gly Gly Tyr Arg Thr Val Gly Glu Met Leu Phe Lys Asp Glu Arg 
                165                 170                 175     


Tyr Ala Lys His Lys Arg Asn Lys Gly Glu Ala Tyr Leu Asn Thr Val 
            180                 185                 190         


Ser Arg Asp Met Ile Glu Ala Glu Ile His Ala Val Phe Ala Ser Gln 
        195                 200                 205             


Arg Asn Phe Gly Met Pro Phe Ala Asn Glu Lys Ile Glu Asn Glu Tyr 
    210                 215                 220                 


Thr Asp Ile Ala Leu Ser Gln Arg Pro Phe Asp Leu Gly Pro Gly Glu 
225                 230                 235                 240 


Gly Pro Glu Asn Cys Lys Ser Pro Tyr Ala Gly Lys Gln Ile Lys Asn 
                245                 250                 255     


Met Ile Gly Gln Cys Thr Phe Phe Pro Glu Glu Lys Arg Ala Val Lys 
            260                 265                 270         


Ala Ser Tyr Ser Phe Gln Leu Phe Ser Leu Leu Gln Gly Ile Asn Asn 
        275                 280                 285             


Ile Thr Leu Val Ser Ser Asp Gly Asn Thr Phe Pro Leu Ser Gln Glu 
    290                 295                 300                 


Gln Arg Lys Thr Leu Lys Asp Tyr Cys Phe Lys Thr Ala Asn Val Thr 
305                 310                 315                 320 


Tyr Ala Ser Ile Arg Lys Val Leu Gln Ile Pro Gln Glu Tyr Thr Phe 
                325                 330                 335     


Lys Asn Ile Thr Tyr Thr Asp Asp Gly Ile Glu Lys Ser Glu Lys Lys 
            340                 345                 350         


Thr Lys Phe Gln His Leu Lys Pro Tyr His Glu Met Lys Lys Val Leu 
        355                 360                 365             


Gly Ser Ala Ile Gln Thr Leu Thr Pro Glu Glu Leu Asp Glu Ile Gly 
    370                 375                 380                 


Glu Ile Phe Thr Phe Tyr Lys Asp Glu Thr Ile Ile Ile Lys Gln Leu 
385                 390                 395                 400 


Glu Glu Thr Glu Ile Asp Lys Ser Leu Tyr Asp Leu Leu Leu Gln Leu 
                405                 410                 415     


Pro Thr Phe Glu Lys Ala Gly His Ile Ser Thr Lys Ala Cys Lys Met 
            420                 425                 430         


Ile Ile Pro Phe Leu Glu Glu Gly Lys Thr Tyr Asp Lys Ala Cys Glu 
        435                 440                 445             


Cys Ala Gly Leu Asn Phe Lys Ala His Thr Asn Met Gln Lys Gln Met 
    450                 455                 460                 


Leu Leu Pro Ala Lys Ser Asp Glu Leu Asn Asp Val Val Asn Pro Val 
465                 470                 475                 480 


Val Arg Arg Ala Ile Ser Gln Thr Ile Lys Val Val Asn Ala Ile Ile 
                485                 490                 495     


Arg Glu Met Gly Thr Ser Pro Thr Tyr Ile Asn Ile Glu Leu Ala Arg 
            500                 505                 510         


Glu Leu Ser Lys Ser Lys Lys Glu Arg Asp Glu Ile Asn Lys Lys Tyr 
        515                 520                 525             


Lys Leu Asn Gln Ala Glu Asn Glu Arg Leu Lys Asn Glu Ile Ile Asp 
    530                 535                 540                 


Asn Phe Lys Ile Lys Pro Asn Gly Gln Asp Ile Ile Lys Leu Lys Leu 
545                 550                 555                 560 


Trp His Glu Gln Asp Asp Gly Ile Cys Pro Tyr Thr Gly Val Arg Ile 
                565                 570                 575     


Lys Tyr Glu Asn Leu Phe Asp Asp Gly Tyr Ala Glu Ile Asp His Ile 
            580                 585                 590         


Ile Pro Tyr Ser Leu Ser Phe Asp Asp Ser Phe Asn Asn Lys Ile Leu 
        595                 600                 605             


Thr Thr Ala Thr Gln Asn Arg Gln Lys Gly Asn Arg Ile Pro Leu Glu 
    610                 615                 620                 


Tyr Leu Pro Glu Ser Glu Lys Asp Gly Phe Arg Val Trp Val Asn Ser 
625                 630                 635                 640 


Asn Ile Arg Asn His Arg Lys Arg Gln Asn Leu Leu Lys Glu His Phe 
                645                 650                 655     


Thr Glu Ala Asp Lys Lys Glu Phe Lys Ser Arg Asn Leu Asn Asp Thr 
            660                 665                 670         


Arg Tyr Leu Asn Lys Leu Leu Leu Asn Tyr Ile Asn Asp Asn Leu Leu 
        675                 680                 685             


Phe Thr Asp Phe Tyr Asn Gly Lys Lys Lys His Val Thr Ser Val Asn 
    690                 695                 700                 


Gly Ala Val Thr Ala Tyr Ile Arg Lys Arg Trp Gly Ile Asn Lys Asn 
705                 710                 715                 720 


Arg Glu Asp Gly Asp Leu His His Ala Val Asp Ala Ala Val Ile Ala 
                725                 730                 735     


Cys Val Thr Gln Gly Ile Ile Asn Arg Ile Thr Ala Tyr Ser Ala Arg 
            740                 745                 750         


Asn Glu Ala Pro Tyr Asp Leu Asn Thr Glu Thr Gly Glu Ile Lys Glu 
        755                 760                 765             


Arg Phe Pro Leu Pro Tyr Pro Asn Phe Lys Lys Glu Leu Asp Ala Arg 
    770                 775                 780                 


Thr Thr Ile Glu Asp Glu Lys Arg Leu Lys Arg Val Leu Met Ser Phe 
785                 790                 795                 800 


Ser Asn Tyr Ser Tyr Asp Asp Val Glu Lys Ala Lys Pro Cys Phe Val 
                805                 810                 815     


Ser Arg Thr Pro His Arg Lys Val Thr Gly Ala Ala His Glu Glu Thr 
            820                 825                 830         


Ile Arg Ser Gly Lys Val Glu Gly Phe Thr Val Ser Lys Thr Glu Leu 
        835                 840                 845             


Thr Lys Leu Lys Leu Thr Asn Gly Glu Ile Glu Gly Tyr Tyr Glu Pro 
    850                 855                 860                 


Glu Ser Asp Thr Leu Leu Tyr Asn Ala Leu Leu Gln Arg Leu Asn Glu 
865                 870                 875                 880 


Phe Gly Gly Asp Ala Lys Ala Ala Phe Ala Asp Glu Phe His Lys Pro 
                885                 890                 895     


Lys Ala Asp Gly Thr Pro Gly Pro Val Val Lys Lys Val Lys Thr Ile 
            900                 905                 910         


Lys Arg Ser Ser Ser Asn Val Lys Ala Arg Asn Lys Asn Gly Leu Gly 
        915                 920                 925             


Val Ala Asp Asn Gly Ser Met Ile Arg Ile Asp Val Phe Tyr Val Glu 
    930                 935                 940                 


Asn Glu Gly Tyr Tyr Phe Val Pro Ile Tyr Val Ala Asp Thr Val Lys 
945                 950                 955                 960 


Glu Met Pro Asn Glu Ala Cys Pro Phe Gly Lys Asp Lys Lys Lys Ile 
                965                 970                 975     


Met Thr Asp Asp Asp Phe Leu Phe Ser Leu Tyr Pro Asn Asp Leu Ile 
            980                 985                 990         


Lys Ile Thr Ala Lys Lys Asp Met  Lys Phe Ser Leu Ala  Asn Lys Glu 
        995                 1000                 1005             


Ser Gly  Leu Pro Lys Asn Lys  Tyr Ala Asn Glu Val  Leu Val Tyr 
    1010                 1015                 1020             


Tyr Ser  Ala Ala Asp Ile Ser  Asn Ala Ser Ile Ser  Val Ile Met 
    1025                 1030                 1035             


His Asp  Asn Ala Tyr Lys Phe  Arg Gly Cys Gly Ile  Lys Ser Leu 
    1040                 1045                 1050             


Val Lys  Leu Glu Lys Tyr Thr  Val Asp Pro Leu Gly  Asn Val Ser 
    1055                 1060                 1065             


Lys Val  Asn Arg Glu Lys Arg  Met Tyr Phe Lys 
    1070                 1075                 


<210>  587
<211>  1079
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  587

Met Arg Leu Gly Leu Asp Ile Gly Thr Ser Ser Ile Gly Trp Trp Leu 
1               5                   10                  15      


Tyr Glu Thr Asp Gly Ala Gly Ser Asp Ala Arg Ile Thr Gly Val Val 
            20                  25                  30          


Asp Gly Gly Val Arg Ile Phe Ser Asp Gly Arg Asp Pro Lys Ser Gly 
        35                  40                  45              


Ala Ser Leu Ala Val Asp Arg Arg Ala Ala Arg Ala Met Arg Arg Arg 
    50                  55                  60                  


Arg Asp Arg Tyr Leu Arg Arg Arg Ala Thr Leu Met Lys Val Leu Ala 
65                  70                  75                  80  


Glu Thr Gly Leu Met Pro Ala Asp Pro Ala Glu Ala Lys Ala Leu Glu 
                85                  90                  95      


Ala Leu Asp Pro Phe Ala Leu Arg Ala Ala Gly Leu Asp Glu Pro Leu 
            100                 105                 110         


Pro Leu Pro His Leu Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg 
        115                 120                 125             


Gly Phe Lys Ser Asn Arg Lys Thr Asp Arg Gly Asp Asn Glu Ser Gly 
    130                 135                 140                 


Lys Ile Lys Asp Ala Thr Ala Arg Leu Asp Met Glu Met Met Ala Asn 
145                 150                 155                 160 


Gly Ala Arg Thr Tyr Gly Glu Phe Leu His Lys Arg Arg Gln Lys Ala 
                165                 170                 175     


Thr Asp Pro Arg His Val Pro Ser Val Arg Thr Arg Leu Ser Ile Ala 
            180                 185                 190         


Asn Arg Gly Gly Pro Asp Gly Lys Glu Glu Ala Gly Tyr Asp Phe Tyr 
        195                 200                 205             


Pro Asp Arg Arg His Leu Glu Glu Glu Phe His Lys Leu Trp Ala Ala 
    210                 215                 220                 


Gln Gly Ala His His Pro Glu Leu Thr Glu Thr Leu Arg Asp Leu Leu 
225                 230                 235                 240 


Phe Glu Lys Ile Phe Phe Gln Arg Pro Leu Lys Glu Pro Glu Val Gly 
                245                 250                 255     


Leu Cys Leu Phe Ser Gly His His Gly Val Pro Pro Lys Asp Pro Arg 
            260                 265                 270         


Leu Pro Lys Ala His Pro Leu Thr Gln Arg Arg Val Leu Tyr Glu Thr 
        275                 280                 285             


Val Asn Gln Leu Arg Val Thr Ala Asp Gly Arg Glu Ala Arg Pro Leu 
    290                 295                 300                 


Thr Arg Glu Glu Arg Asp Gln Val Ile His Ala Leu Asp Asn Lys Lys 
305                 310                 315                 320 


Pro Thr Lys Ser Leu Ser Ser Met Val Leu Lys Leu Pro Ala Leu Ala 
                325                 330                 335     


Lys Val Leu Lys Leu Arg Asp Gly Glu Arg Phe Thr Leu Glu Thr Gly 
            340                 345                 350         


Val Arg Asp Ala Ile Ala Cys Asp Pro Leu Arg Ala Ser Pro Ala His 
        355                 360                 365             


Pro Asp Arg Phe Gly Pro Arg Trp Ser Ile Leu Asp Ala Asp Ala Gln 
    370                 375                 380                 


Trp Glu Val Ile Ser Arg Ile Arg Arg Val Gln Ser Asp Ala Glu His 
385                 390                 395                 400 


Ala Ala Leu Val Asp Trp Leu Thr Glu Ala His Gly Leu Asp Arg Ala 
                405                 410                 415     


His Ala Glu Ala Thr Ala His Ala Pro Leu Pro Asp Gly Tyr Gly Arg 
            420                 425                 430         


Leu Gly Leu Thr Ala Thr Thr Arg Ile Leu Tyr Gln Leu Thr Ala Asp 
        435                 440                 445             


Val Val Thr Tyr Ala Asp Ala Val Lys Ala Cys Gly Trp His His Ser 
    450                 455                 460                 


Asp Gly Arg Thr Gly Glu Cys Phe Asp Arg Leu Pro Tyr Tyr Gly Glu 
465                 470                 475                 480 


Val Leu Glu Arg His Val Ile Pro Gly Ser Tyr His Pro Asp Asp Asp 
                485                 490                 495     


Asp Ile Thr Arg Phe Gly Arg Ile Thr Asn Pro Thr Val His Ile Gly 
            500                 505                 510         


Leu Asn Gln Leu Arg Arg Leu Val Asn Arg Ile Ile Glu Thr His Gly 
        515                 520                 525             


Lys Pro His Gln Ile Val Val Glu Leu Ala Arg Asp Leu Lys Lys Ser 
    530                 535                 540                 


Glu Glu Gln Lys Arg Ala Asp Ile Lys Arg Ile Arg Asp Thr Thr Glu 
545                 550                 555                 560 


Ala Ala Lys Lys Arg Ser Glu Lys Leu Glu Glu Leu Glu Ile Glu Asp 
                565                 570                 575     


Asn Gly Arg Asn Arg Met Leu Leu Arg Leu Trp Glu Asp Leu Asn Pro 
            580                 585                 590         


Asp Asp Ala Met Arg Arg Phe Cys Pro Tyr Thr Gly Thr Arg Ile Ser 
        595                 600                 605             


Ala Ala Met Ile Phe Asp Gly Ser Cys Asp Val Asp His Ile Leu Pro 
    610                 615                 620                 


Tyr Ser Arg Thr Leu Asp Asp Ser Phe Pro Asn Arg Thr Leu Cys Leu 
625                 630                 635                 640 


Arg Glu Ala Asn Arg Gln Lys Arg Asn Gln Thr Pro Trp Gln Ala Trp 
                645                 650                 655     


Gly Asp Thr Pro His Trp His Ala Ile Ala Ala Asn Leu Lys Asn Leu 
            660                 665                 670         


Pro Glu Asn Lys Arg Trp Arg Phe Ala Pro Asp Ala Met Thr Arg Phe 
        675                 680                 685             


Glu Gly Glu Asn Gly Phe Leu Asp Arg Ala Leu Lys Asp Thr Gln Tyr 
    690                 695                 700                 


Leu Ala Arg Ile Ser Arg Ser Tyr Leu Asp Thr Leu Phe Thr Lys Gly 
705                 710                 715                 720 


Gly His Val Trp Val Val Pro Gly Arg Phe Thr Glu Met Leu Arg Arg 
                725                 730                 735     


His Trp Gly Leu Asn Ser Leu Leu Ser Asp Ala Gly Arg Gly Ala Val 
            740                 745                 750         


Lys Ala Lys Asn Arg Thr Asp His Arg His His Ala Ile Asp Ala Ala 
        755                 760                 765             


Val Ile Ala Ala Thr Asp Pro Gly Leu Leu Asn Arg Ile Ser Arg Ala 
    770                 775                 780                 


Ala Gly Gln Gly Glu Ala Ala Gly Gln Ser Ala Glu Leu Ile Ala Arg 
785                 790                 795                 800 


Asp Thr Pro Pro Pro Trp Glu Gly Phe Arg Asp Asp Leu Arg Val Arg 
                805                 810                 815     


Leu Asp Arg Ile Ile Val Ser His Arg Ala Asp His Gly Arg Ile Asp 
            820                 825                 830         


His Ala Ala Arg Lys Gln Gly Arg Asp Ser Thr Ala Gly Gln Leu His 
        835                 840                 845             


Gln Glu Thr Ala Tyr Ser Ile Val Asp Asp Ile His Val Ala Ser Arg 
    850                 855                 860                 


Thr Asp Leu Leu Ser Leu Lys Pro Ala Gln Leu Leu Asp Glu Pro Gly 
865                 870                 875                 880 


Arg Ser Gly Gln Val Arg Asp Pro Gln Leu Arg Lys Ala Leu Arg Val 
                885                 890                 895     


Ala Thr Gly Gly Lys Thr Gly Lys Asp Phe Glu Asn Ala Leu Arg Tyr 
            900                 905                 910         


Phe Ala Ser Lys Pro Gly Pro Tyr Gln Ala Ile Arg Arg Val Arg Ile 
        915                 920                 925             


Ile Lys Pro Leu Gln Ala Gln Ala Arg Val Pro Val Pro Ala Gln Asp 
    930                 935                 940                 


Pro Ile Lys Ala Tyr Gln Gly Gly Ser Asn His Leu Phe Glu Ile Trp 
945                 950                 955                 960 


Arg Leu Pro Asp Gly Glu Ile Glu Ala Gln Val Ile Thr Ser Phe Glu 
                965                 970                 975     


Ala His Thr Leu Glu Gly Glu Lys Arg Pro His Pro Ala Ala Lys Arg 
            980                 985                 990         


Leu Leu Arg Val His Lys Gly Asp  Met Val Ala Leu Glu  Arg Asp Gly 
        995                 1000                 1005             


Arg Arg  Val Val Gly His Val  Gln Lys Met Asp Ile  Ala Asn Gly 
    1010                 1015                 1020             


Leu Phe  Ile Val Pro His Asn  Glu Ala Asn Ala Asp  Thr Arg Asn 
    1025                 1030                 1035             


Asn Asp  Lys Ser Asp Pro Phe  Lys Trp Ile Gln Ile  Gly Ala Arg 
    1040                 1045                 1050             


Pro Ala  Ile Ala Ser Gly Ile  Arg Arg Val Ser Val  Asp Glu Ile 
    1055                 1060                 1065             


Gly Arg  Leu Arg Asp Gly Gly  Thr Arg Pro Ile 
    1070                 1075                 


<210>  588
<211>  1079
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  588

Met Asp Glu Glu Ala Lys Ile Gln Leu Ser Lys Glu Ser Tyr Glu Glu 
1               5                   10                  15      


Glu Leu Glu Ser Leu Leu Glu Lys Ser Gly Glu Glu Phe Arg Asp Val 
            20                  25                  30          


Phe Leu Gln Ala Lys Lys Val Tyr Asp Ala Ile Leu Leu Ser Asp Ile 
        35                  40                  45              


Leu Ser Thr Lys Lys Gln Asn Ser Lys Ala Lys Leu Ser Leu Gly Met 
    50                  55                  60                  


Ile Glu Arg Tyr Asp Ser His Lys Lys Asp Leu Glu Glu Leu Lys Gln 
65                  70                  75                  80  


Phe Val Lys Ala Asn Leu Pro Glu Lys Thr Ala Ile Phe Phe Lys Asp 
                85                  90                  95      


Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr Gln 
            100                 105                 110         


Glu Asp Phe Tyr Lys Phe Leu Lys Lys Glu Leu Asn Gly Ile Ala Gly 
        115                 120                 125             


Ser Glu Arg Phe Met Glu Lys Val Asp Gln Glu Asn Phe Leu Leu Lys 
    130                 135                 140                 


Gln Arg Thr Thr Ala Asn Gly Val Ile Pro His Gln Val His Leu Thr 
145                 150                 155                 160 


Glu Leu Lys Ala Ile Ile Glu Arg Gln Lys Pro Tyr Tyr Pro Phe Leu 
                165                 170                 175     


Glu Glu Ala Arg Asp Lys Met Ile Arg Leu Leu Thr Phe Arg Ile Pro 
            180                 185                 190         


Tyr Tyr Val Gly Pro Leu Ala Gln Gly Glu Glu Thr Ser Ser Phe Ala 
        195                 200                 205             


Trp Leu Glu Arg Lys Thr Pro Glu Lys Val Thr Pro Trp Asn Ala Thr 
    210                 215                 220                 


Glu Val Ile Asp Tyr Ser Ala Ser Ala Met Lys Phe Ile Gln Arg Met 
225                 230                 235                 240 


Ile Asn Tyr Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys His 
                245                 250                 255     


Ser Ile Leu Tyr Gln Lys Tyr Thr Ile Phe Asn Glu Leu Thr Lys Val 
            260                 265                 270         


Ala Tyr Lys Asp Glu Arg Gly Ile Lys His Gln Phe Ser Ser Lys Glu 
        275                 280                 285             


Lys Arg Glu Ile Phe Lys Glu Leu Phe Gln Lys Gln Arg Lys Val Thr 
    290                 295                 300                 


Val Lys Lys Leu Gln Gln Phe Leu Ser Ala Asn Tyr Asn Ile Glu Asp 
305                 310                 315                 320 


Ala Glu Ile Leu Gly Val Asp Lys Ala Phe Asn Ser Ser Tyr Ala Thr 
                325                 330                 335     


Tyr His Asp Phe Leu Asp Leu Ala Lys Pro Asn Thr Glu Arg Val Ala 
            340                 345                 350         


Glu Leu Leu Glu Gln Pro Glu Met Asn Ala Met Phe Glu Asp Ile Val 
        355                 360                 365             


Lys Ile Leu Thr Ile Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu 
    370                 375                 380                 


Lys Lys Tyr Gln Ser Val Leu Gly Asp Gly Phe Phe Lys Lys Leu Val 
385                 390                 395                 400 


Lys Lys His Tyr Thr Gly Trp Gly Arg Leu Ser Glu Arg Leu Ile Asn 
                405                 410                 415     


Gly Ile Arg Asp Lys Lys Thr Asn Lys Thr Ile Leu Asp Tyr Leu Ile 
            420                 425                 430         


Asp Asp Asp Asp Phe Pro Tyr Asn Arg Asn Arg Asn Phe Met Gln Leu 
        435                 440                 445             


Ile Asn Asp Asp Ser Leu Ser Phe Lys Glu Glu Leu Ala Asn Glu Leu 
    450                 455                 460                 


Ala Leu Ala Gly Asn Gln Ser Leu Leu Glu Val Val Glu Ala Leu Pro 
465                 470                 475                 480 


Gly Ser Pro Ala Ile Lys Lys Gly Ile Trp Gln Thr Leu Lys Ile Val 
                485                 490                 495     


Glu Glu Leu Ile Glu Ile Ile Gly Tyr Asn Pro Lys Asn Ile Val Val 
            500                 505                 510         


Glu Met Ala Arg Glu Asn Gln Arg Thr Asn Arg Ser Lys Pro Arg Leu 
        515                 520                 525             


Lys Ala Leu Glu Glu Ala Leu Lys Ser Phe Asp Ser Pro Leu Leu Lys 
    530                 535                 540                 


Glu Gln Pro Val Asp Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu 
545                 550                 555                 560 


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp 
                565                 570                 575     


Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser 
            580                 585                 590         


Phe Ile Val Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys 
        595                 600                 605             


Glu Asn Arg Leu Lys Met Asp Asp Val Pro Asp Gln Lys Val Val Ile 
    610                 615                 620                 


Arg Met Arg Arg Tyr Trp Glu Lys Leu Leu Arg Ala Asn Leu Ile Ser 
625                 630                 635                 640 


Glu Arg Lys Phe Ala Tyr Leu Thr Lys Leu Glu Leu Thr Pro Glu Asp 
                645                 650                 655     


Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
            660                 665                 670         


Lys His Val Ala Ala Ile Leu Asp Gln Tyr Phe Asn Gln Pro Glu Glu 
        675                 680                 685             


Ser Lys Asn Lys Gly Ile Arg Ile Ile Thr Leu Lys Ser Ser Leu Val 
    690                 695                 700                 


Ser Gln Phe Arg Lys Thr Phe Gly Ile His Lys Val Arg Glu Ile Asn 
705                 710                 715                 720 


Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala Ile 
                725                 730                 735     


Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr Gly 
            740                 745                 750         


Asn Tyr Thr Lys Phe Asn Leu Ala Thr Glu Asn Lys Ala Thr Ala Lys 
        755                 760                 765             


Lys Glu Phe Tyr Ser Asn Ile Leu Arg Phe Phe Glu Lys Glu Glu Tyr 
    770                 775                 780                 


Ser Tyr Asp Glu Asn Gly Glu Ile Phe Trp Asp Lys Ala Arg His Ile 
785                 790                 795                 800 


Pro Gln Ile Lys Lys Val Ile Ser Ser His Gln Val Asn Ile Val Lys 
                805                 810                 815     


Lys Val Glu Val Gln Thr Gly Gly Phe Tyr Lys Glu Thr Val Asn Pro 
            820                 825                 830         


Lys Gly Lys Pro Asp Lys Leu Ile Gln Arg Lys Ala Gly Trp Asp Val 
        835                 840                 845             


Ser Lys Tyr Gly Gly Phe Gly Ser Pro Val Val Ala Tyr Ala Val Ala 
    850                 855                 860                 


Phe Ile Tyr Glu Lys Gly Lys Ala Arg Lys Lys Ala Lys Ala Ile Glu 
865                 870                 875                 880 


Gly Ile Thr Ile Met Lys Gln Ser Leu Phe Glu Gln Asp Pro Ile Gly 
                885                 890                 895     


Phe Leu Ser Asn Lys Gly Tyr Ser Asn Val Thr Lys Phe Ile Lys Leu 
            900                 905                 910         


Ser Lys Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Met Val 
        915                 920                 925             


Ala Ser His Lys Glu Ala Gln Lys Ala Asn Ser Phe Ile Leu Pro Glu 
    930                 935                 940                 


Lys Leu Val Thr Leu Leu Phe Tyr Ala Gln His Tyr Asp Glu Ile Ala 
945                 950                 955                 960 


His Lys Glu Ser Phe Asp Tyr Val Asn Asp His Leu Ser Glu Phe Arg 
                965                 970                 975     


Glu Ile Leu Asp Gln Val Ile Asp Phe Ser Asn Arg Tyr Thr Ile Ala 
            980                 985                 990         


Ala Lys Asn Thr Glu Lys Ile Ala  Glu Leu Phe Glu Gln  Asn Gln Glu 
        995                 1000                 1005             


Ser Thr  Val Gln Ser Leu Ser  Gln Ser Phe Ile Asn  Leu Met Gln 
    1010                 1015                 1020             


Leu Asn  Ala Met Gly Ala Pro  Ala Asp Phe Lys Phe  Phe Asp Val 
    1025                 1030                 1035             


Ile Ile  Pro Arg Lys Arg Tyr  Pro Ser Leu Thr Glu  Ile Trp Glu 
    1040                 1045                 1050             


Ser Thr  Ile Ile Tyr Gln Ser  Ile Thr Gly Leu Arg  Glu Thr Arg 
    1055                 1060                 1065             


Thr Arg  Met Ala Thr Leu Trp  Asp Gly Glu Gln 
    1070                 1075                 


<210>  589
<211>  1079
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  589

Met Asp Glu Glu Ala Lys Ile Gln Leu Ser Lys Glu Ser Tyr Glu Glu 
1               5                   10                  15      


Glu Leu Glu Ser Leu Leu Glu Lys Ser Gly Glu Glu Phe Arg Asp Val 
            20                  25                  30          


Phe Leu Gln Ala Lys Lys Val Tyr Asp Ala Ile Leu Leu Ser Asp Ile 
        35                  40                  45              


Leu Ser Thr Lys Lys Gln Asn Ser Lys Ala Lys Leu Ser Leu Gly Met 
    50                  55                  60                  


Ile Glu Arg Tyr Asp Ser His Lys Lys Asp Leu Glu Glu Leu Lys Gln 
65                  70                  75                  80  


Phe Val Lys Ala Asn Leu Pro Glu Lys Thr Ala Ile Phe Phe Lys Asp 
                85                  90                  95      


Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr Gln 
            100                 105                 110         


Glu Asp Phe Tyr Lys Phe Leu Lys Lys Glu Leu Asn Gly Ile Ala Gly 
        115                 120                 125             


Ser Glu Arg Phe Met Glu Lys Val Asp Gln Glu Asn Phe Leu Leu Lys 
    130                 135                 140                 


Gln Arg Thr Thr Ala Asn Gly Val Ile Pro His Gln Val His Leu Thr 
145                 150                 155                 160 


Glu Leu Lys Ala Ile Ile Glu Arg Gln Lys Pro Tyr Tyr Pro Ser Leu 
                165                 170                 175     


Glu Glu Ala Arg Asp Lys Met Ile Arg Leu Leu Thr Phe Arg Ile Pro 
            180                 185                 190         


Tyr Tyr Val Gly Pro Leu Ala Gln Gly Glu Glu Thr Ser Ser Phe Ala 
        195                 200                 205             


Trp Leu Glu Arg Lys Thr Pro Glu Lys Val Thr Pro Trp Asn Ala Thr 
    210                 215                 220                 


Glu Val Ile Asp Tyr Ser Ala Ser Ala Met Lys Phe Ile Gln Arg Met 
225                 230                 235                 240 


Ile Asn Tyr Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys His 
                245                 250                 255     


Ser Ile Leu Tyr Gln Lys Tyr Thr Ile Phe Asn Glu Leu Thr Lys Val 
            260                 265                 270         


Ala Tyr Lys Asp Glu Arg Gly Ile Lys His Gln Phe Ser Ser Lys Glu 
        275                 280                 285             


Lys Arg Glu Ile Phe Lys Glu Leu Phe Gln Lys Gln Arg Lys Val Thr 
    290                 295                 300                 


Val Lys Lys Leu Gln Gln Phe Leu Ser Ala Asn Tyr Asn Ile Glu Asp 
305                 310                 315                 320 


Ala Glu Ile Leu Gly Val Asp Lys Ala Phe Asn Ser Ser Tyr Ala Thr 
                325                 330                 335     


Tyr His Asp Phe Leu Asp Leu Ala Lys Pro Asn Thr Glu Arg Val Ala 
            340                 345                 350         


Glu Leu Leu Glu Gln Pro Glu Met Asn Ala Met Phe Glu Asp Ile Val 
        355                 360                 365             


Lys Ile Leu Thr Ile Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu 
    370                 375                 380                 


Lys Lys Tyr Gln Ser Val Leu Gly Asp Gly Phe Phe Lys Lys Leu Val 
385                 390                 395                 400 


Lys Lys His Tyr Thr Gly Trp Gly Arg Leu Ser Glu Arg Leu Ile Asn 
                405                 410                 415     


Gly Ile Arg Asp Lys Lys Thr Asn Lys Thr Ile Leu Asp Tyr Leu Ile 
            420                 425                 430         


Asp Asp Asp Asp Phe Pro Tyr Asn Arg Asn Arg Asn Phe Met Gln Leu 
        435                 440                 445             


Ile Asn Asp Asp Ser Leu Ser Phe Lys Glu Glu Leu Ala Asn Glu Leu 
    450                 455                 460                 


Ala Leu Ala Gly Asn Gln Ser Leu Leu Glu Val Val Glu Ala Leu Leu 
465                 470                 475                 480 


Gly Ser Pro Ala Ile Lys Lys Gly Ile Trp Gln Thr Leu Lys Ile Val 
                485                 490                 495     


Glu Glu Leu Ile Glu Ile Ile Gly Tyr Asn Pro Lys Asn Ile Val Val 
            500                 505                 510         


Glu Met Ala Arg Glu Asn Gln Arg Thr Asn Arg Ser Lys Pro Arg Leu 
        515                 520                 525             


Lys Ala Leu Glu Glu Ala Leu Lys Ser Phe Asp Ser Pro Leu Leu Lys 
    530                 535                 540                 


Glu Gln Pro Val Asp Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu 
545                 550                 555                 560 


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp 
                565                 570                 575     


Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser 
            580                 585                 590         


Phe Ile Val Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys 
        595                 600                 605             


Glu Asn Arg Leu Lys Met Asp Asp Val Pro Asp Gln Lys Val Val Ile 
    610                 615                 620                 


Arg Met Arg Arg Tyr Trp Glu Lys Leu Leu Arg Ala Asn Leu Ile Ser 
625                 630                 635                 640 


Glu Arg Lys Phe Ala Tyr Leu Thr Lys Leu Glu Leu Thr Pro Glu Asp 
                645                 650                 655     


Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
            660                 665                 670         


Lys His Val Ala Ala Ile Leu Asp Gln Tyr Phe Asn Gln Pro Glu Glu 
        675                 680                 685             


Ser Lys Asn Lys Gly Ile Arg Ile Ile Thr Leu Lys Ser Ser Leu Val 
    690                 695                 700                 


Ser Gln Phe Arg Lys Thr Phe Gly Ile Asn Lys Val Arg Glu Ile Asn 
705                 710                 715                 720 


Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala Ile 
                725                 730                 735     


Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr Gly 
            740                 745                 750         


Asn Tyr Thr Lys Phe Asn Leu Ala Thr Glu Asn Lys Ala Thr Ala Lys 
        755                 760                 765             


Lys Glu Phe Tyr Ser Asn Ile Leu Arg Phe Phe Glu Lys Glu Glu Tyr 
    770                 775                 780                 


Ser Tyr Asp Glu Asn Gly Glu Ile Phe Trp Asp Lys Ala Arg His Ile 
785                 790                 795                 800 


Pro Gln Ile Lys Lys Val Ile Ser Ser His Gln Val Asn Ile Val Lys 
                805                 810                 815     


Lys Val Glu Val Gln Thr Gly Gly Phe Tyr Lys Glu Thr Val Asn Pro 
            820                 825                 830         


Lys Gly Lys Pro Asp Lys Leu Ile Gln Arg Lys Ala Gly Trp Asp Val 
        835                 840                 845             


Ser Lys Tyr Gly Gly Phe Gly Ser Pro Val Val Ala Tyr Ala Val Ala 
    850                 855                 860                 


Phe Ile Tyr Glu Lys Gly Lys Ala Arg Lys Lys Ala Lys Ala Ile Glu 
865                 870                 875                 880 


Gly Ile Thr Ile Met Lys Gln Ser Leu Phe Glu Gln Asp Pro Ile Gly 
                885                 890                 895     


Phe Leu Ser Asn Lys Gly Tyr Ser Asn Val Thr Lys Phe Ile Lys Leu 
            900                 905                 910         


Ser Lys Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Met Val 
        915                 920                 925             


Ala Ser His Lys Glu Ala Gln Lys Ala Asn Ser Phe Ile Leu Pro Glu 
    930                 935                 940                 


Lys Leu Val Thr Leu Leu Tyr His Ala Gln His Tyr Asp Glu Ile Ala 
945                 950                 955                 960 


His Lys Glu Ser Phe Asp Tyr Val Asn Asp His Leu Ser Glu Phe Arg 
                965                 970                 975     


Glu Ile Leu Asp Gln Val Ile Asp Phe Ser Asn Arg Tyr Thr Ile Ala 
            980                 985                 990         


Ala Lys Asn Thr Glu Lys Ile Ala  Glu Leu Phe Glu Gln  Asn Gln Glu 
        995                 1000                 1005             


Ser Thr  Val Gln Ser Leu Ser  Gln Ser Phe Ile Asn  Leu Met Gln 
    1010                 1015                 1020             


Leu Asn  Ala Met Gly Ala Pro  Ala Asp Phe Lys Phe  Phe Asp Val 
    1025                 1030                 1035             


Ile Ile  Pro Arg Lys Arg Tyr  Pro Ser Leu Thr Glu  Ile Trp Glu 
    1040                 1045                 1050             


Ser Thr  Ile Ile Tyr Gln Ser  Ile Thr Gly Leu Arg  Glu Thr Arg 
    1055                 1060                 1065             


Thr Arg  Met Ala Thr Leu Trp  Asp Gly Glu Gln 
    1070                 1075                 


<210>  590
<211>  1080
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  590

Met Lys Thr Ser Phe Ala Phe Asp Ile Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Cys Val Leu Ala Leu Asp Asn Ala Asn Leu Pro Tyr Arg Ile Leu Asp 
            20                  25                  30          


Ala Gly Val Arg Ile Phe Ser Asp Gly Arg Glu Pro Lys Ser Gly Thr 
        35                  40                  45              


Ser Leu Ala Glu Gly Arg Arg Leu Ala Arg Gly Met Ser Arg Arg Arg 
    50                  55                  60                  


Asp Arg Tyr Lys Arg Arg Arg Lys Ala Val Ile Arg Thr Leu Ile Glu 
65                  70                  75                  80  


Tyr Gly Leu Met Pro Asp Asp Pro Ala Ala Arg Arg Ala Leu Val Ala 
                85                  90                  95      


Glu Thr Asn Asp Lys Ala Pro Gly Lys Ile Ala Ser Asp Val Tyr Ala 
            100                 105                 110         


Leu Arg Ala Arg Ala Leu Asp Asp Lys Leu Pro Leu His His Ile Gly 
        115                 120                 125             


Arg Ala Leu Phe His Ile Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg 
    130                 135                 140                 


Lys Thr Asp Lys Arg Asp Asn Asp Gln Gly Lys Ile Ala Ser Gly Ile 
145                 150                 155                 160 


Ser Arg Leu Arg Asp Lys Leu Ala Ser Glu Gly Met Arg Thr Leu Gly 
                165                 170                 175     


Glu Tyr Leu Asn His Arg His Gln Ala Gly Asn Trp Val Arg Val Arg 
            180                 185                 190         


Pro Ser Arg Val Pro Gly Lys Asp Asp Lys Glu Glu Asp Gly Tyr Gly 
        195                 200                 205             


Phe Tyr Pro Glu Arg Ala Leu Leu Gln Asp Glu Phe Asn Ala Ile Trp 
    210                 215                 220                 


Thr Ala Gln Ala Ala His Tyr Pro Asp Val Leu Thr Glu Glu Arg Arg 
225                 230                 235                 240 


Thr His Leu Glu Thr Ile Ile Phe Phe Gln Arg Pro Leu Lys Pro Val 
                245                 250                 255     


Gln Val Gly Lys Cys Ser Phe Asn Pro Gly Glu Glu Arg Leu Pro Arg 
            260                 265                 270         


Ala His Pro Leu Phe Gln Glu Phe Arg Leu Tyr Lys Glu Val Asn Glu 
        275                 280                 285             


Leu Glu Leu Val Asp Arg Asn Gln Asp His His Lys Leu Ser Leu Asp 
    290                 295                 300                 


Gln Arg Asp Ala Leu Ile Ala Gln Leu Lys Thr Gln Arg Glu Ala Ala 
305                 310                 315                 320 


Tyr Ser Ala Leu Arg Lys Thr Ala Arg Ala Pro Leu Gly Thr Arg Phe 
                325                 330                 335     


Asn Lys Glu Thr Glu Ser Arg Thr Lys Met Lys Gly Asp Glu Val Ala 
            340                 345                 350         


Ala Glu Leu Ser Ala Lys Thr Arg Phe Gly Gln Ala Trp Ile Ser Met 
        355                 360                 365             


Pro Leu Glu Glu Arg Trp Ile Ile Val Asp Thr Leu Arg Arg Val Glu 
    370                 375                 380                 


Asp Pro Leu Glu Leu Thr Ala Trp Leu Glu Gln His Tyr Gly Leu Asp 
385                 390                 395                 400 


Gly Glu His Ile Asp Ala Ile Leu Ala Ala Arg Leu Pro Glu Gly Tyr 
                405                 410                 415     


Gly Arg Leu Gly Glu Thr Ala Leu Thr Glu Ile Leu Val Glu Leu Lys 
            420                 425                 430         


Ala Ala Val Ile Ser Glu Ala Glu Ala Ala Arg Arg Ala Gly Tyr Asp 
        435                 440                 445             


His Ala Leu Leu Gln Arg Gly Ala Gly Leu Glu Ala Leu Pro Lys Tyr 
    450                 455                 460                 


Gln Glu Val Ile Ala Arg Arg Ile Pro Pro Gly Thr Gly Asn Pro Asp 
465                 470                 475                 480 


Asp Pro Tyr Asp Val Glu Lys Gly Arg Ile Thr Asn Pro Thr Val His 
                485                 490                 495     


Ile Gly Leu Asn Gln Leu Gln Lys Val Leu Asn Ala Leu Ile Arg Arg 
            500                 505                 510         


Tyr Gly Lys Pro Glu Gln Ile Ala Ile Glu Leu Ala Arg Glu Leu Lys 
        515                 520                 525             


Leu Asn Asp Glu Gln Lys Arg Glu Lys Asn Ile Glu Ile Ala Arg Asn 
    530                 535                 540                 


Thr Arg Ala Ala Glu Gln Arg Ser Ala Lys Leu Val Glu Leu Gly Val 
545                 550                 555                 560 


Pro Asp Thr Gly Tyr Asn Arg Val Leu Leu Lys Leu Trp Glu Glu Leu 
                565                 570                 575     


Asn Leu Arg Ala Pro Glu Asn Arg Val Cys Ile Tyr Cys Gly Glu Pro 
            580                 585                 590         


Ile Asn Ile Glu Thr Leu Phe Ser Ser Ala Val Asp Ile Asp His Ile 
        595                 600                 605             


Leu Pro Trp Ser Lys Thr Leu Asp Asp Ser Gln Ala Asn Arg Leu Val 
    610                 615                 620                 


Cys His Val Ala Cys Asn Arg Gln Lys Ala Asn Tyr Ala Pro Ala Glu 
625                 630                 635                 640 


Val Pro Gln Trp Gln Gly Arg Tyr Asp Asp Ile Leu Ala Arg Ala Asn 
                645                 650                 655     


Thr Leu Pro Leu Asn Lys Arg Trp Arg Phe Gly Arg Asp Ala Met Lys 
            660                 665                 670         


Lys Phe Ala Asp Glu Glu Thr Phe Leu Ala Arg Gln Leu Thr Asp Thr 
        675                 680                 685             


Gln Tyr Leu Ser Arg Leu Ala His Ala Tyr Met Ala Ala Leu Tyr Pro 
    690                 695                 700                 


Asp Glu Glu Pro Asp Ala Gln Gly Val Leu Ala Arg Lys Asn His Val 
705                 710                 715                 720 


Arg Val Ile Thr Gly Arg Met Thr Glu Met Leu Arg Arg Gln Trp Gly 
                725                 730                 735     


Leu Asn Ser Leu Leu Ala Asp His Asn Gln Lys Asn Arg Asn Asp His 
            740                 745                 750         


Arg His His Ala Ile Asp Ala Ala Val Val Gly Val Thr Thr Arg Ala 
        755                 760                 765             


Leu Leu Gln Arg Ile Ala Thr Val Ala Gly Gly Arg Glu Ala Gln Ala 
    770                 775                 780                 


Leu Glu Glu Thr Ile Gly Lys Ile Glu Pro Pro Trp Pro Gly Phe Arg 
785                 790                 795                 800 


Asp Glu Leu Gln Ala Val Ile Lys Arg Ile Val Val Ser His Lys Pro 
                805                 810                 815     


Asp His Gly Thr Leu Pro Lys Pro Gly Gln Gly Gly Gln Thr Ala Gly 
            820                 825                 830         


Gln Leu His Asn Asp Thr Ala Tyr Gly Leu Thr Gly Glu Thr Asp Ser 
        835                 840                 845             


Arg Gly Thr Pro Ile Val Val Arg Arg Lys Pro Phe Thr Ser Leu Thr 
    850                 855                 860                 


Glu Lys Asp Leu Pro Ser Ile Arg Asp Arg Gln Leu Ala Gln Ala Leu 
865                 870                 875                 880 


Gly Ser Ala Ile Ala Gly Arg Ser Gly Lys Glu Leu Gln Ala Ala Leu 
                885                 890                 895     


Glu Gln Phe Arg Asp Arg Asp Asp Tyr Lys Ser Ile Arg His Val Arg 
            900                 905                 910         


Val Val Glu Pro Leu Asn Val Ile Pro Ile Arg Asp Gly Thr Gly Arg 
        915                 920                 925             


Ala Tyr Lys Ala Tyr Lys Gly Asp Ala Asn Tyr Arg Tyr Asp Val Trp 
    930                 935                 940                 


Glu Leu Pro Asn Gly Lys Trp Met His Glu Val Val Ser Met Phe Asp 
945                 950                 955                 960 


Ala His Asp Arg Ser Trp Thr Ser Ala Val His Ile His His Pro Ala 
                965                 970                 975     


Ala Arg Arg Val Leu Ser Leu Lys Gln Asn Asp Met Val Ala Tyr Asp 
            980                 985                 990         


His Pro Gln Asp Gly Pro Thr Ile  Gly Arg Val Val Lys  Phe Ser Gln 
        995                 1000                 1005             


Ser Gly  Gln Met Thr Phe Ala  Pro His Lys Glu Ala  Gly Ala Leu 
    1010                 1015                 1020             


Lys Ala  Arg Asp Ala Asp Pro  Glu Asp Arg Phe Arg  Tyr Phe Tyr 
    1025                 1030                 1035             


Lys Ser  Gly Ser Ala Met Lys  Asp Ile Gly Leu Arg  Gln Val Arg 
    1040                 1045                 1050             


Val Asp  Glu Ile Gly Arg Val  Phe Asp Pro Gly Pro  Arg Asp Lys 
    1055                 1060                 1065             


Glu Ala  Arg Asn Ala Arg Lys  Lys Lys Ser Thr Asp  
    1070                 1075                 1080 


<210>  591
<211>  1080
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  591

Met Ala Lys Ser Phe Ala Phe Asp Met Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Leu Gly Leu Asp Ser Ala Gly Asp Pro Asp Ser Ile Glu Gly 
            20                  25                  30          


Ala Gly Val Arg Ile Phe Ser Asp Gly Arg Glu Pro Lys Ser Gly Ala 
        35                  40                  45              


Ser Leu Ala Glu Gly Arg Arg Ile Ala Arg Gly Met Ser Arg Arg Arg 
    50                  55                  60                  


Glu Arg Tyr Lys Ala Arg Arg Lys Ala Leu Ile Arg Thr Leu Gly Asp 
65                  70                  75                  80  


Tyr Gly Leu Met Pro Leu Glu Arg Ala Ala Arg Arg Ala Leu Val Ala 
                85                  90                  95      


Glu Thr Asn Asp Arg Ser Phe Gly Leu Val Ala Gln Asp Val Tyr Ala 
            100                 105                 110         


Leu Arg Ala Arg Ala Leu Val Glu Lys Leu Pro Pro Tyr His Leu Gly 
        115                 120                 125             


Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg 
    130                 135                 140                 


Lys Thr Asp Gly Arg Asp Asn Glu Leu Gly Met Ile Ala Thr Gly Ile 
145                 150                 155                 160 


Ala Ala Leu Asp Ala Gln Leu Asn Gly Arg Thr Leu Gly Gln Tyr Leu 
                165                 170                 175     


His Glu Met Arg Gly Ala Asp Pro Arg Gln Arg Gly Gln Val Arg Val 
            180                 185                 190         


Arg Pro His Leu Glu Ile Asn Asp Lys Gly Arg Glu Ile Ala Ala Tyr 
        195                 200                 205             


Gly Phe Tyr Pro Gln Arg Ala Met Leu Glu Arg Glu Phe Asn Ala Ile 
    210                 215                 220                 


Trp Ala Ala Gln Ala Pro His His Pro Glu Leu Leu Ser Glu Glu Arg 
225                 230                 235                 240 


Arg Ala His Leu Phe Gln Val Ile Phe Tyr Gln Arg Pro Leu Lys Gln 
                245                 250                 255     


Pro Asp Pro Gly Arg Cys Ser Phe Asn Pro Ala Glu Pro Arg Leu Ala 
            260                 265                 270         


Lys Ala His Pro Leu Phe Gln Arg Phe Arg Leu Val Lys Glu Ile Asn 
        275                 280                 285             


Glu Leu Arg Ile Val Thr Gly Ser Asn Ser Gln Gly Val Pro Leu Thr 
    290                 295                 300                 


Leu Glu Gln Arg Asp Ile Leu Leu Thr Leu Leu Arg Asp Lys Lys Ala 
305                 310                 315                 320 


Ser Ser Phe Lys Thr Leu Leu Ala Lys Ile Ile Lys Gly Gln Pro Glu 
                325                 330                 335     


Ala Arg Phe Asn Lys Ala Ser Asp Ser Arg Asp Lys Met Lys Gly Asp 
            340                 345                 350         


Glu Leu Ala Ala Thr Met Ser Asp Lys Thr Arg Phe Gly Gln Ala Trp 
        355                 360                 365             


Phe Ala Met Pro Glu Ala Glu Gln Trp Arg Ile Ile Ala Lys Leu Arg 
    370                 375                 380                 


Asp Ile Asp Asp Pro Leu Glu Leu Asn Ala Trp Leu Glu Ser Glu Tyr 
385                 390                 395                 400 


Gly Leu Ala Pro Glu Arg Arg Asp Ala Val Ala Asn Ala Leu Leu Pro 
                405                 410                 415     


Gln Gly Tyr Gly Arg Leu Gly Glu Thr Ala Leu Arg Thr Met Leu Asp 
            420                 425                 430         


Tyr Leu Glu Ala Ala Val Ile Ser Glu Ala Glu Ala Ala Ala Gln Ala 
        435                 440                 445             


Gly Tyr Asp His Ala Leu Ala Arg Arg Gly Ala Gly Leu Asp Gln Leu 
    450                 455                 460                 


Pro Lys Tyr Gln Glu Val Leu Gly Arg Arg Ile Pro Pro Gly Ser Gly 
465                 470                 475                 480 


Glu Pro Asn Asp Pro Tyr Asp Ile Arg Met Gly Arg Ile Ser Asn Pro 
                485                 490                 495     


Thr Val His Ile Ala Leu Asn Gln Leu Arg Arg Val Val Asn Ala Leu 
            500                 505                 510         


Ile Arg Lys Tyr Gly Lys Pro Ala Glu Ile Ala Ile Glu Leu Ala Arg 
        515                 520                 525             


Asp Leu Lys Leu Ser Glu Glu Gln Lys Arg Glu Val Asn Ala Thr Ile 
    530                 535                 540                 


Ala Lys Asn Thr Arg Asp Ala Glu Arg Arg Ala Val Arg Leu Ala Asp 
545                 550                 555                 560 


Glu Gly Ile Asp Asn Thr Gly His Asn Arg Ala Leu Leu Arg Phe Trp 
                565                 570                 575     


Glu Glu Leu Ala Ala Asp Pro Leu Asp Arg Val Cys Val Tyr Cys Gly 
            580                 585                 590         


Gly Gly Ile Gly Ala Ala Ser Leu Phe Asn Gly Ser Val Asp Ile Asp 
        595                 600                 605             


His Ile Leu Pro Trp Ser Arg Thr Leu Asp Asp Ser Arg Ala Asn Arg 
    610                 615                 620                 


Ile Leu Val His Asn Ser Cys Asn Arg Leu Lys Arg Asn Phe Ala Pro 
625                 630                 635                 640 


Phe Glu Val Pro Glu Trp Arg Glu Ile Tyr Gln Asp Ile Leu Val Arg 
                645                 650                 655     


Ala Ala Lys Leu Pro Ala Asn Lys Ser Trp Arg Phe Ala Pro Asp Ala 
            660                 665                 670         


Met Asp Arg Phe Glu Gly Glu Lys Asp Phe Leu Ala Arg Gln Leu Thr 
        675                 680                 685             


Asp Thr Gln Tyr Leu Ala Arg Met Ala His Glu Tyr Leu Ala Ala Leu 
    690                 695                 700                 


Tyr Pro Asp Glu Glu Pro Asp Glu Trp Gly Val Phe Ala Arg Gly Asn 
705                 710                 715                 720 


His Val Arg Val Val Pro Gly Arg Leu Thr Glu Met Leu Arg Arg Ala 
                725                 730                 735     


Trp Gly Leu Asn Ala Ile Leu Ser Asp Asp Asn Arg Lys Asn Arg Asn 
            740                 745                 750         


Asp His Arg His His Ala Ile Asp Ala Val Val Val Gly Val Thr Thr 
        755                 760                 765             


Arg Asp Met Leu Asn Lys Ile Ser Gln Ala Ala Gly Arg Gly Ala Asp 
    770                 775                 780                 


Met Gly Ala Ala Asp Ala Leu Arg Arg Phe Asp Pro Pro Trp Pro Ser 
785                 790                 795                 800 


Phe His Gln Ala Val Arg Ala Ala Val Glu Arg Ile Leu Val Ser His 
                805                 810                 815     


Arg Ala Asp His Gly Thr Leu Pro Gly Thr Asp Gly Gln Gly Ser Thr 
            820                 825                 830         


Ala Gly Gln Leu His Asn Asp Thr Ala Tyr Gly Leu Thr Gly Glu Thr 
        835                 840                 845             


Arg Lys Gly Leu Ala Val Val Val Arg Arg Lys Pro Tyr Leu Ser Leu 
    850                 855                 860                 


Glu Pro Arg Asp Ile Glu Thr Ile Arg Asp Pro Gln Leu Arg Asp His 
865                 870                 875                 880 


Leu Ala Thr Glu Thr Tyr Gly Leu Ala Gly Ala Glu Phe Thr Ala Ala 
                885                 890                 895     


Leu Lys Arg Leu Gln Gln Thr Asp Lys Phe Tyr Ala Gly Ile Arg Arg 
            900                 905                 910         


Val Arg Val Thr Glu Ala Leu Ser Val Ile Pro Ile Arg Asp Ala Thr 
        915                 920                 925             


Gly His Ala Tyr Lys Ala Tyr Lys Gly Asp Ala Asn Phe Arg Tyr Asp 
    930                 935                 940                 


Val Trp Glu Leu Pro Asn Gly Lys Trp Val Pro Glu Val Ile Ser Met 
945                 950                 955                 960 


Phe Asp Ala His Asp Arg Gly Trp Val Ser Gln Val His Ala Ala Asn 
                965                 970                 975     


Pro Ala Ala Arg Arg Val Leu Ser Leu Arg Gln Asn Asp Met Val Ala 
            980                 985                 990         


Tyr Gln His Pro Glu Gln Gly Leu  Val Tyr Gly Arg Val  Val Lys Phe 
        995                 1000                 1005             


Gly Gln  Ser Gly Gln Met Thr  Phe Ala Ala His His  Glu Gly Gly 
    1010                 1015                 1020             


Ser Leu  Lys Ser Arg Asp Ala  Asp Arg Asp Asp Pro  Phe Lys Tyr 
    1025                 1030                 1035             


Phe Tyr  Lys Ser Ala Gly Thr  Met Lys Asp Ile Gly  Leu Arg Gln 
    1040                 1045                 1050             


Ile Arg  Val Asp Glu Ile Gly  Arg Val Gln Asp Pro  Gly Pro Gln 
    1055                 1060                 1065             


Asp Arg  Asp Ser Lys Arg Val  Arg Lys Ser Asp Arg  
    1070                 1075                 1080 


<210>  592
<211>  1081
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  592

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Lys Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Asn Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Ile Ser Asp Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Thr Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Val Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Gln Pro Leu Thr Asp Thr Gln Arg Ala Lys 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Val Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asn Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Gln Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Ala Tyr Ala Asp Glu Glu Asp Trp Thr Val Ile Asp 
            980                 985                 990         


Glu Ser Phe Arg Phe Lys Phe Val  Leu Tyr Ser Asn Asp  Leu Ile Lys 
        995                 1000                 1005             


Val Gln  Leu Lys Lys Asp Ser  Phe Leu Gly Tyr Phe  Ser Gly Leu 
    1010                 1015                 1020             


Asp Arg  Ala Thr Gly Ala Ile  Ser Leu Arg Glu His  Asp Leu Glu 
    1025                 1030                 1035             


Lys Ser  Lys Gly Lys Asp Gly  Met His Arg Ile Gly  Val Lys Thr 
    1040                 1045                 1050             


Ala Leu  Ser Phe Gln Lys Tyr  Gln Ile Asp Glu Met  Gly Lys Glu 
    1055                 1060                 1065             


Ile Arg  Pro Cys Arg Leu Lys  Lys Arg Pro Pro Val  Arg 
    1070                 1075                 1080     


<210>  593
<211>  1081
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  593

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Ala Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ser Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Arg Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Asp Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Ile Ser Asp Asp Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Thr Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Val Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Glu Leu Asp Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Val Gln Pro Glu Ile Leu Glu Val Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Asp Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Ala Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Glu Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp His Met Leu Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Arg Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asn 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Ala Gly Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Ala Tyr Ala Asp Glu Glu Gly Trp Thr Val Ile Asp 
            980                 985                 990         


Glu Ser Phe Arg Phe Lys Phe Val  Leu Tyr Ser Asn Asp  Leu Ile Lys 
        995                 1000                 1005             


Val Gln  Leu Lys Lys Asp Ser  Phe Leu Gly Tyr Phe  Ser Gly Leu 
    1010                 1015                 1020             


Asp Arg  Ala Thr Gly Ala Ile  Ser Leu Arg Glu His  Asp Leu Glu 
    1025                 1030                 1035             


Lys Ser  Lys Gly Lys Asp Gly  Met His Arg Ile Gly  Val Lys Thr 
    1040                 1045                 1050             


Ala Leu  Ser Phe Gln Lys Tyr  Gln Ile Asp Glu Met  Gly Lys Glu 
    1055                 1060                 1065             


Ile Arg  Leu Cys Arg Leu Lys  Lys Arg Pro Pro Val  Arg 
    1070                 1075                 1080     


<210>  594
<211>  1081
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  594

Met Thr Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Ala Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Val Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Val Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Ile Ser Asp Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Ala Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


Tyr Cys Thr Phe Glu Pro Thr Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Ile Asp Met Pro Tyr Lys Asn Lys Leu Thr Tyr Ala Lys Ala Arg 
305                 310                 315                 320 


Glu Leu Leu Gly Leu Asp Asp Thr Ala Phe Phe Lys Gly Leu Arg Tyr 
                325                 330                 335     


Gly Lys Asp Asn Ala Glu Ser Ala Thr Leu Met Glu Met Lys Ala Tyr 
            340                 345                 350         


His Ala Ile Arg Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys Lys 
        355                 360                 365             


Ser Pro Leu Ser Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr Ala 
    370                 375                 380                 


Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys Gly 
385                 390                 395                 400 


Cys Leu Gln Pro Glu Ile Leu Asp Ala Leu Leu Lys His Ile Asn Phe 
                405                 410                 415     


Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val Pro 
            420                 425                 430         


Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Thr Tyr 
        435                 440                 445             


Gly Asp His Tyr Gly Glu Lys Asn Ser Glu Glu Met Leu Tyr Leu Pro 
    450                 455                 460                 


Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala Leu 
465                 470                 475                 480 


Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly Ser 
                485                 490                 495     


Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser Phe 
            500                 505                 510         


Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys Asp 
        515                 520                 525             


Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe Val 
    530                 535                 540                 


Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu Gln 
545                 550                 555                 560 


Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Ala Ile Asp Ile Arg Arg 
                565                 570                 575     


Leu Asn Gly Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe Ser 
            580                 585                 590         


Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly Ser 
        595                 600                 605             


Glu Asn Gln Asn Lys Gly Asn Arg Thr Pro Tyr Glu Tyr Leu Asn Gly 
    610                 615                 620                 


Lys Asp Asn Ser Arg Glu Trp Leu Glu Phe Lys Ala Arg Val Glu Thr 
625                 630                 635                 640 


Ser Arg Phe Pro His Ser Lys Lys Gln Arg Val Leu Leu Gln Lys Phe 
                645                 650                 655     


Asp Glu Lys Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr Val 
            660                 665                 670         


Asn Arg Phe Leu Cys Gln Phe Val Ala Asp His Met Gln Leu Thr Gly 
        675                 680                 685             


Lys Gly Lys Arg Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn Leu 
    690                 695                 700                 


Leu Arg Gly Phe Trp Gly Leu His Lys Val Arg Ala Glu Asn Asp Arg 
705                 710                 715                 720 


His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala Met 
                725                 730                 735     


Gln Gln Lys Ile Thr Cys Phe Val Arg Tyr Lys Glu Met Asn Ala Phe 
            740                 745                 750         


Asp Gly Lys Thr Val Asp Lys Glu Thr Gly Glu Val Leu His Gln Lys 
        755                 760                 765             


Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met Ile 
    770                 775                 780                 


Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala Asp 
785                 790                 795                 800 


Thr Pro Glu Lys Leu Arg Ala Leu Leu Ala Glu Lys Leu Ser Ser Arg 
                805                 810                 815     


Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg Ala 
            820                 825                 830         


Pro Asn Arg Lys Met Ser Gly Ala His Lys Asp Thr Leu Arg Ser Ala 
        835                 840                 845             


Lys Arg Phe Val Lys His Asn Glu Lys Ile Ser Val Lys Arg Val Trp 
    850                 855                 860                 


Leu Thr Glu Ile Lys Leu Ala Asp Leu Glu Asn Met Val Asn Tyr Lys 
865                 870                 875                 880 


Asn Gly Arg Glu Ile Glu Leu Tyr Glu Ala Leu Lys Ala Arg Leu Gly 
                885                 890                 895     


Ala Tyr Gly Gly Asn Ala Lys Gln Ala Phe Asp Pro Lys Asp Asn Pro 
            900                 905                 910         


Phe Tyr Lys Lys Gly Gly Gln Leu Val Lys Ala Val Arg Val Glu Lys 
        915                 920                 925             


Thr Gln Glu Ser Gly Val Leu Leu Asn Lys Lys Asn Ala Tyr Thr Ile 
    930                 935                 940                 


Ala Asp Asn Gly Asp Met Val Arg Val Asp Val Phe Cys Lys Val Asp 
945                 950                 955                 960 


Lys Lys Gly Lys Asn Gln Tyr Phe Ile Val Pro Ile Tyr Ala Trp Gln 
                965                 970                 975     


Val Ala Glu Asn Ile Leu Pro Asp Ile Asp Cys Lys Gly Tyr Arg Ile 
            980                 985                 990         


Asp Asp Ser Tyr Thr Phe Cys Phe  Ser Leu His Lys Tyr  Asp Leu Ile 
        995                 1000                 1005             


Ala Phe  Gln Lys Asp Glu Lys  Ser Lys Val Glu Phe  Ala Tyr Tyr 
    1010                 1015                 1020             


Ile Asn  Cys Asp Ser Ser Asn  Gly Arg Phe Tyr Leu  Ala Trp His 
    1025                 1030                 1035             


Asp Lys  Gly Ser Lys Glu Gln  Gln Phe Arg Ile Ser  Thr Gln Asn 
    1040                 1045                 1050             


Leu Val  Leu Met Gln Lys Tyr  Gln Ile Asp Glu Leu  Gly Lys Glu 
    1055                 1060                 1065             


Ile Arg  Pro Cys Arg Leu Lys  Lys Arg Pro Pro Val  Arg 
    1070                 1075                 1080     


<210>  595
<211>  1081
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  595

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Ala Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ser Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asn Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Ala Gly Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Ala Tyr Ala Asp Glu Glu Gly Trp Thr Val Ile Asp 
            980                 985                 990         


Glu Ser Phe Arg Phe Lys Phe Val  Leu Tyr Ser Asn Asp  Leu Ile Lys 
        995                 1000                 1005             


Val Gln  Leu Lys Lys Asp Ser  Phe Leu Gly Tyr Phe  Ser Gly Leu 
    1010                 1015                 1020             


Asp Arg  Ala Thr Gly Ala Ile  Ser Leu Arg Glu His  Asp Leu Glu 
    1025                 1030                 1035             


Lys Ser  Lys Gly Lys Asp Gly  Met His Arg Ile Gly  Val Lys Thr 
    1040                 1045                 1050             


Ala Leu  Ser Phe Gln Lys Tyr  Gln Ile Asp Glu Met  Gly Lys Glu 
    1055                 1060                 1065             


Ile Arg  Leu Cys Arg Leu Lys  Lys Arg Pro Pro Val  Arg 
    1070                 1075                 1080     


<210>  596
<211>  1081
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  596

Met Leu Lys Arg Val Leu Gly Ile Asp Val Gly Ile Ser Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Leu Glu Phe Gln Gly Asp Ile Asp Asn Lys Asp Leu Pro 
            20                  25                  30          


Ser Gly Lys Ile Leu Ala Ser Gly Val Arg Val Phe Thr Gln Ala Glu 
        35                  40                  45              


Asn Pro Lys Asn Gly Glu Ser Leu Ala Lys Pro Arg Arg Asp Ala Arg 
    50                  55                  60                  


Leu Ala Arg Arg Arg Thr Arg Arg Arg Ala Gln Arg Leu Thr Asp Ile 
65                  70                  75                  80  


Lys Asp Leu Phe Phe Lys Tyr Lys Leu Val Thr Gly Ile Ser Arg Asn 
                85                  90                  95      


Lys Ile Asn Ser Ile Tyr Lys Thr Glu Glu Asn Lys Lys Asp Pro Trp 
            100                 105                 110         


Thr Leu Arg Lys Glu Ala Leu Tyr Arg Lys Leu Asp Gly Glu Glu Phe 
        115                 120                 125             


Ala Arg Val Leu Thr His Ile Ala Lys His Arg Gly Phe Lys Ser Met 
    130                 135                 140                 


Arg Lys Ser Glu Glu Glu Gly Ser Asp Thr Lys Ala Lys Glu Thr Gln 
145                 150                 155                 160 


Arg Val Leu Thr Glu Ile Gly Lys Asn Lys Asp Thr Leu Lys Thr Gly 
                165                 170                 175     


Ile Trp Gln Thr Ala Gly Glu Met Ile Ala Ser Arg Pro Lys Gly Asp 
            180                 185                 190         


Ala Arg Arg Asn Lys Thr Asp Ser Tyr Lys Asn Ser Ile Ser Arg Glu 
        195                 200                 205             


Leu Leu Gln Asp Glu Thr Lys Ile Ile Phe Glu Lys Gln Arg Gly Met 
    210                 215                 220                 


Asn Ser Gln Phe Thr Ser Ala Asp Leu Glu Lys Glu Tyr Ser Asp Ile 
225                 230                 235                 240 


Ala Phe His Gln Ala Pro Ile Gln Ser Met Leu Lys Met Val Gly Gly 
                245                 250                 255     


Cys Thr Phe Glu Lys Asp Glu Glu Gly Lys Arg Leu Lys Arg Ala Pro 
            260                 265                 270         


Lys Asn Ser Tyr Ser Ala Glu Val Phe Thr Ala Leu Thr Lys Ile Asn 
        275                 280                 285             


Asn Leu Val Leu Lys Thr Lys Thr Ala Asn Ile Asn Asp Arg Phe Leu 
    290                 295                 300                 


Thr Thr Glu Glu Arg Lys Lys Ala Leu Glu Leu Cys His Ser Asn Ala 
305                 310                 315                 320 


Lys Val Thr Phe Lys Gln Leu Arg Gly Glu Leu Gly Leu Pro Glu Asp 
                325                 330                 335     


Trp Ile Phe Asn Thr Val Asp Tyr Ala Lys Lys Pro Lys Lys Thr Ala 
            340                 345                 350         


Val Lys Glu Thr Glu Pro Glu Ser Ala Cys Phe Phe Gln Met Arg Gly 
        355                 360                 365             


Tyr Asn Glu Ile Arg Lys Ser Val Gly Lys Asp Leu Trp Thr Glu Ile 
    370                 375                 380                 


Ser Met Lys Lys Glu Ile Leu Asn Ser Ile Val Thr Thr Leu Ala Thr 
385                 390                 395                 400 


Glu Lys Ser Asp Glu Ala Ile Lys Ala Gln Leu Lys Lys Ser Glu Val 
                405                 410                 415     


Pro Asp Glu Ile Ala Glu Lys Val Leu Gly Leu Asn Phe Arg Gly Phe 
            420                 425                 430         


Leu His Leu Ser Ile Lys Ala Leu Asp Lys Ile Ile Pro Phe Met Met 
        435                 440                 445             


Ala Gly Asp Lys Tyr Thr Asp Ala Cys Asp Lys Ala Gly Tyr Lys Glu 
    450                 455                 460                 


Gln Ile Ser Gly Ile Lys Lys Ile Phe Leu Arg Lys Leu Asn Asp Glu 
465                 470                 475                 480 


Glu Asn Glu Gln Ile Thr Asn Pro Val Ala Lys Arg Ala Ile Thr Gln 
                485                 490                 495     


Phe Arg Lys Val Ile Asn Ala Ile Ile Arg Glu Tyr Gly Lys Phe Asp 
            500                 505                 510         


Gln Met Asn Ile Glu Leu Ala Arg Asp Leu Ala Lys Ser Tyr Asp Asp 
        515                 520                 525             


Arg Lys Lys Ile Lys Glu Gly Gln Glu Asp Phe Arg Ala Glu Lys Asp 
    530                 535                 540                 


Arg Ala Ala Gln Arg Cys Lys Asp Asn Gly Leu Asp Pro Asn Ser Lys 
545                 550                 555                 560 


Asp Asn Leu Ser Lys Tyr Arg Leu Trp Glu Gln Gln Asp Asn Gly Phe 
                565                 570                 575     


Cys Ile Tyr Cys Gly Lys Pro Ile Glu Leu Gln Arg Leu Ala Glu Gln 
            580                 585                 590         


Gly Tyr Cys Glu Val Asp His Ile Leu Pro Tyr Ser Arg Ser Leu Asp 
        595                 600                 605             


Asp Ser Gln His Asn Lys Val Leu Ala His Ile His Asp Asn Gln Gln 
    610                 615                 620                 


Lys Lys Asn Gln Ile Pro Phe Glu His Phe Gly Gly Asp Gly Pro Lys 
625                 630                 635                 640 


Trp Glu Glu Phe Val Gly Arg Leu Asn Ser Gln Phe Lys Asn Leu Pro 
                645                 650                 655     


Lys Ser Lys Leu Asn Arg Leu Val Lys Arg Lys Phe Asp Glu Lys Asp 
            660                 665                 670         


Met Glu Gly Phe Lys Glu Arg Asn Ile Asn Asp Thr Arg Tyr Ile Ser 
        675                 680                 685             


Arg Phe Ile Lys Asn Phe Val Glu Asn His Leu Glu Phe Ala Glu Ser 
    690                 695                 700                 


Ala Gln Lys Gln Lys Val Phe Val Arg Ser Gly Ser Leu Thr Ala Phe 
705                 710                 715                 720 


Leu Arg His Gln Trp Gly Leu Ile Lys Lys Arg Asp Glu Ser Asp Arg 
                725                 730                 735     


His His Ala Leu Asp Ala Leu Val Thr Ala Cys Ala Thr Gln Ala Met 
            740                 745                 750         


Val Lys Ser Thr Ala Ile Thr Ala Gln Lys Met Glu Ser Tyr Asn Trp 
        755                 760                 765             


Ile Asn Lys Glu Lys Pro Arg Met Pro Leu Pro Trp Leu Asn Phe Arg 
    770                 775                 780                 


Asp Asp Val Gln Lys Ser Leu Asp Ala Val Phe Val Ser Arg Ala Pro 
785                 790                 795                 800 


Arg His Lys Val Gly Gly Ala Ala His Lys Glu Thr Ile Arg Ser Ile 
                805                 810                 815     


Arg Lys Cys Gly Glu Glu Asn Leu Ile Ile Glu Lys Thr Pro Leu Val 
            820                 825                 830         


Asp Ile Ala Thr Lys Ser Gly Ile Lys Leu Lys Asp Ile Glu Lys Leu 
        835                 840                 845             


Tyr Lys Lys Glu Gln Asn Arg Lys Leu Tyr Glu Thr Leu Lys Glu Arg 
    850                 855                 860                 


Leu Glu Gln Asn Gly Asn Asp Pro Val Lys Ala Phe Gly Asn Pro Asp 
865                 870                 875                 880 


Asp Pro Val Arg Met Pro Thr Asn Asp Leu Thr Lys Thr Gly Pro Ile 
                885                 890                 895     


Ile Lys Ser Ile Lys Leu Ile Thr Asn Lys Lys Ser Gly Leu Asn Val 
            900                 905                 910         


Arg Ala Gly Ile Ala Asp Asn Gly Glu Ile Ala Arg Leu Asp Val Tyr 
        915                 920                 925             


Glu Arg Lys Gly Lys Phe Tyr Leu Ile Pro Asn Tyr Val Ala Asp Phe 
    930                 935                 940                 


Ala Asn Lys Glu Leu Pro Lys Lys Ala Ile Lys Ala Asn Thr Pro Glu 
945                 950                 955                 960 


Thr Glu Trp Pro Ile Ile Asp Arg Asn Tyr Asn Phe Lys Phe Ser Leu 
                965                 970                 975     


Phe Lys Asp Asp Leu Ile Ser Ile Lys Gln Lys Leu Asp Ser Pro Glu 
            980                 985                 990         


Glu Phe Tyr Tyr Tyr Lys Gly Pro  Asp Val Ser Thr Ala  Ser Ile Thr 
        995                 1000                 1005             


Val Glu  Ser Val Asp Arg Lys  Arg Leu Trp Glu Glu  Lys Lys Lys 
    1010                 1015                 1020             


Gly Lys  Ile Glu Asn Val Phe  His Thr Arg Ile Gly  Thr Lys Thr 
    1025                 1030                 1035             


Leu Lys  Ser Phe Arg Lys Phe  Asn Leu Asp Ile Leu  Gly Thr Lys 
    1040                 1045                 1050             


Ala Pro  Val Glu Ile Lys Lys  Glu Thr Trp Val Gly  Val Ile Arg 
    1055                 1060                 1065             


Lys Asn  Gly Val Ala Asn Asn  Thr Gly Asn Lys Ala  Met 
    1070                 1075                 1080     


<210>  597
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  597

Met Ala Ala Phe Lys Pro Asn Pro Met Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Lys Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Asp Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Val Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Cys Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Asn Leu Leu Phe Glu Lys Gln Lys Glu Phe Ser Asn 
    210                 215                 220                 


Pro His Val Ser Asp Ser Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Ala Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Pro Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Thr Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Lys Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Val Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Cys Lys Lys Asn Ala Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Cys Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Val 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Glu Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp His Ile Leu Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Arg Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Ala His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Ile Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Gly Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Asp Ala Leu Lys Ala Gln Leu Glu Thr His Lys 
                885                 890                 895     


Asp Asn Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Ile Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Gly Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Ala Phe Lys Asp Glu Glu Asp Trp Thr Val Met Asp 
            980                 985                 990         


Asp Ser Phe Glu Phe Arg Phe Val  Leu Tyr Ala Asn Asp  Leu Ile Lys 
        995                 1000                 1005             


Leu Thr  Ala Lys Lys Asn Glu  Phe Leu Gly Tyr Phe  Val Ser Leu 
    1010                 1015                 1020             


Asn Arg  Ala Thr Gly Ala Ile  Asp Ile Arg Thr His  Asp Thr Asp 
    1025                 1030                 1035             


Ser Thr  Lys Gly Lys Asn Gly  Ile Phe Gln Ser Val  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Asn Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  598
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  598

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asn Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Ser Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Val Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Val 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Cys Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp His Ile Leu Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Arg Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Lys Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  599
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  599

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Glu 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  600
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  600

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  601
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  601

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Ala Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Val Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  602
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  602

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Thr Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asn 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Leu Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Gly Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Asn Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Ile Ser Asp Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Thr Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Thr Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Glu Leu Asp Asp Ile Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Asn 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Asp Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile His Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Thr Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Ile Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Met Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  603
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  603

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Asn Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Ile Ser Asp Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Thr Thr 
    290                 295                 300                 


Leu Ile Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Glu Leu Asp Asn Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Val Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Asp Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Thr Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Glu Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp His Met Leu Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Arg Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  604
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  604

Met Ala Ala Phe Lys Pro Asn Pro Met Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Ile Val Glu Ile Asp Glu Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Ala Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Thr His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Asn Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Asn Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Asp Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Thr Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Val Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Asp Leu Asp Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Val Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Asn Arg Tyr Asp Glu Ala Cys Thr Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ser Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Ala Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Ile Asn Arg Phe Leu Cys Gln Phe Val Ala Asp His Met Leu Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Arg Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Ile Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Ala His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Lys Tyr Val Thr Pro Leu Phe Ile Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Ile Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val His Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Ile Val Arg Val Asp Val Phe Glu Lys Gly Gly Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Thr Val Met Asp 
            980                 985                 990         


Asp Ser Phe Glu Phe Lys Phe Val  Leu Tyr Ala Asn Asp  Leu Ile Lys 
        995                 1000                 1005             


Leu Thr  Ala Lys Lys Asn Glu  Phe Leu Gly Tyr Phe  Val Ser Leu 
    1010                 1015                 1020             


Asn Arg  Ala Thr Gly Ala Ile  Asp Ile Arg Thr His  Asp Thr Asp 
    1025                 1030                 1035             


Ser Thr  Lys Gly Lys Asn Gly  Ile Phe Gln Ser Val  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  605
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  605

Met Ala Ala Phe Lys Pro Asn Ser Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Lys Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Ala Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Thr Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asn 
                85                  90                  95      


Phe Asp Lys Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Leu Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Gly Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Asn Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Ile Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Thr Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Thr Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Ser Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Thr Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Val 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp His Ile Leu Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Arg Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Leu Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  606
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  606

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Val Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Ile Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  607
<211>  1082
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  607

Met Arg Val Leu Gly Leu Asp Ala Gly Ile Ala Ser Leu Gly Trp Ala 
1               5                   10                  15      


Leu Ile Glu Ile Glu Glu Ser Asn Arg Gly Glu Leu Ser Gln Gly Thr 
            20                  25                  30          


Ile Ile Gly Ala Gly Thr Trp Met Phe Asp Ala Pro Glu Glu Lys Thr 
        35                  40                  45              


Gln Ala Gly Ala Lys Leu Lys Ser Glu Gln Arg Arg Thr Phe Arg Gly 
    50                  55                  60                  


Gln Arg Arg Val Val Arg Arg Arg Arg Gln Arg Met Asn Glu Val Arg 
65                  70                  75                  80  


Arg Ile Leu His Ser His Gly Leu Leu Pro Ser Ser Asp Arg Asp Ala 
                85                  90                  95      


Leu Lys Gln Pro Gly Leu Asp Pro Trp Arg Ile Arg Ala Glu Ala Leu 
            100                 105                 110         


Asp Arg Leu Leu Gly Pro Val Glu Leu Ala Val Ala Leu Gly His Ile 
        115                 120                 125             


Ala Arg His Arg Gly Phe Lys Ser Asn Ser Lys Gly Ala Lys Thr Asn 
    130                 135                 140                 


Asp Pro Ala Asp Asp Thr Ser Lys Met Lys Arg Ala Val Asn Glu Thr 
145                 150                 155                 160 


Arg Glu Lys Leu Ala Arg Phe Gly Ser Ala Ala Lys Met Leu Val Glu 
                165                 170                 175     


Asp Glu Ser Phe Val Leu Arg Gln Thr Pro Thr Lys Asn Gly Ala Ser 
            180                 185                 190         


Glu Ile Val Arg Arg Phe Arg Asn Arg Glu Gly Asp Tyr Ser Arg Ser 
        195                 200                 205             


Leu Leu Arg Asp Asp Leu Ala Ala Glu Met Arg Ala Leu Phe Thr Ala 
    210                 215                 220                 


Gln Ala Arg Phe Gln Ser Ala Ile Ala Thr Ala Asp Leu Gln Thr Ala 
225                 230                 235                 240 


Phe Thr Lys Ala Ala Phe Phe Gln Arg Pro Leu Gln Asp Ser Glu Lys 
                245                 250                 255     


Leu Val Gly Pro Cys Pro Phe Glu Val Asp Glu Lys Arg Ala Pro Lys 
            260                 265                 270         


Arg Gly Tyr Ser Phe Glu Leu Phe Arg Phe Leu Ser Arg Leu Asn His 
        275                 280                 285             


Val Thr Leu Arg Asp Gly Lys Gln Glu Arg Thr Leu Thr Arg Asp Glu 
    290                 295                 300                 


Leu Ala Leu Ala Ala Ala Asp Phe Gly Ala Ala Ala Lys Val Ser Phe 
305                 310                 315                 320 


Thr Ala Leu Arg Lys Lys Leu Lys Leu Pro Glu Thr Thr Val Phe Val 
                325                 330                 335     


Gly Val Lys Ala Asp Glu Glu Ser Lys Leu Asp Val Val Ala Arg Ser 
            340                 345                 350         


Gly Lys Ala Ala Glu Gly Thr Ala Arg Leu Arg Ser Val Ile Val Asp 
        355                 360                 365             


Ala Leu Gly Glu Leu Ala Trp Gly Ala Leu Leu Cys Ser Pro Glu Lys 
    370                 375                 380                 


Leu Asp Lys Ile Ala Glu Val Ile Ser Phe Arg Ser Asp Ile Gly Arg 
385                 390                 395                 400 


Ile Ser Glu Gly Leu Ala Gln Ala Gly Cys Asn Ala Pro Leu Val Asp 
                405                 410                 415     


Ala Leu Thr Ala Ala Ala Ser Asp Gly Arg Phe Asp Pro Phe Thr Gly 
            420                 425                 430         


Ala Gly His Ile Ser Ser Lys Ala Ala Arg Asn Ile Leu Ser Gly Leu 
        435                 440                 445             


Arg Gln Gly Met Thr Tyr Asp Lys Ala Cys Cys Ala Ala Asp Tyr Asp 
    450                 455                 460                 


His Thr Ala Ser Arg Glu Arg Gly Ala Phe Asp Val Gly Gly His Gly 
465                 470                 475                 480 


Arg Glu Ala Leu Lys Arg Ile Leu Gln Glu Glu Arg Ile Ser Arg Glu 
                485                 490                 495     


Leu Val Gly Ser Pro Thr Ala Arg Lys Ala Leu Ile Glu Ser Ile Lys 
            500                 505                 510         


Gln Val Lys Ala Ile Val Glu Arg Tyr Gly Val Pro Asp Arg Ile His 
        515                 520                 525             


Val Glu Leu Ala Arg Asp Val Gly Lys Ser Ile Glu Glu Arg Glu Glu 
    530                 535                 540                 


Ile Thr Arg Gly Ile Glu Lys Arg Asn Arg Gln Lys Asp Lys Leu Arg 
545                 550                 555                 560 


Gly Leu Phe Glu Lys Glu Val Gly Arg Pro Pro Gln Asp Gly Ala Arg 
                565                 570                 575     


Gly Lys Glu Glu Leu Leu Arg Phe Glu Leu Trp Ser Glu Gln Met Gly 
            580                 585                 590         


Arg Cys Leu Tyr Thr Asp Asp Tyr Ile Ser Pro Ser Gln Leu Val Ala 
        595                 600                 605             


Thr Asp Asp Ala Val Gln Val Asp His Ile Leu Pro Trp Ser Arg Phe 
    610                 615                 620                 


Ala Asp Asp Ser Tyr Ala Asn Lys Thr Leu Cys Met Ala Lys Ala Asn 
625                 630                 635                 640 


Gln Asp Lys Lys Gly Arg Thr Pro Tyr Glu Trp Phe Lys Ala Glu Lys 
                645                 650                 655     


Thr Asp Thr Glu Trp Asp Ala Phe Ile Val Arg Val Glu Ala Leu Ala 
            660                 665                 670         


Asp Met Lys Gly Phe Lys Lys Arg Asn Tyr Lys Leu Arg Asn Ala Glu 
        675                 680                 685             


Glu Ala Ala Ala Lys Phe Arg Asn Arg Asn Leu Asn Asp Thr Arg Trp 
    690                 695                 700                 


Ala Cys Arg Leu Leu Ala Glu Ala Leu Lys Gln Leu Tyr Pro Lys Gly 
705                 710                 715                 720 


Glu Lys Asp Lys Asp Gly Lys Glu Arg Arg Arg Val Phe Ser Arg Pro 
                725                 730                 735     


Gly Ala Leu Thr Asp Arg Leu Arg Arg Ala Trp Gly Leu Gln Trp Met 
            740                 745                 750         


Lys Lys Ser Thr Lys Gly Asp Arg Ile Pro Asp Asp Arg His His Ala 
        755                 760                 765             


Leu Asp Ala Ile Val Ile Ala Ala Thr Thr Glu Ser Leu Leu Gln Arg 
    770                 775                 780                 


Ala Thr Arg Glu Val Gln Glu Ile Glu Asp Lys Gly Leu His Tyr Asp 
785                 790                 795                 800 


Leu Val Lys Asn Val Thr Pro Pro Trp Pro Gly Phe Arg Glu Gln Ala 
                805                 810                 815     


Val Glu Ala Val Glu Lys Val Phe Val Ala Arg Ala Glu Arg Arg Arg 
            820                 825                 830         


Ala Arg Gly Lys Ala His Asp Ala Thr Ile Arg His Ile Ala Val Arg 
        835                 840                 845             


Glu Gly Glu Gln Arg Val Tyr Glu Arg Arg Lys Val Ala Glu Leu Lys 
    850                 855                 860                 


Leu Ala Asp Leu Asp Arg Val Lys Asp Ala Glu Arg Asn Ala Arg Leu 
865                 870                 875                 880 


Ile Glu Lys Leu Arg Asn Trp Ile Glu Ala Gly Ser Pro Lys Asp Asp 
                885                 890                 895     


Pro Pro Leu Ser Pro Lys Gly Asp Pro Ile Phe Lys Val Arg Leu Val 
            900                 905                 910         


Thr Lys Ser Lys Val Asn Ile Ala Leu Asp Thr Gly Asn Pro Lys Arg 
        915                 920                 925             


Pro Gly Thr Val Asp Arg Gly Glu Met Ala Arg Val Asp Val Phe Arg 
    930                 935                 940                 


Lys Ala Ser Lys Lys Gly Lys Tyr Glu Tyr Tyr Leu Val Pro Ile Tyr 
945                 950                 955                 960 


Pro His Asp Ile Ala Thr Met Lys Thr Pro Pro Ile Arg Ala Val Gln 
                965                 970                 975     


Ala Tyr Lys Pro Glu Asp Glu Trp Pro Glu Met Asp Ser Ser Tyr Glu 
            980                 985                 990         


Phe Cys Trp Ser Leu Val Pro Met  Thr Tyr Leu Gln Val  Ile Ser Ser 
        995                 1000                 1005             


Lys Gly  Glu Ile Phe Glu Gly  Tyr Tyr Arg Gly Met  Asn Arg Ser 
    1010                 1015                 1020             


Val Gly  Ala Ile Gln Leu Ser  Ala His Ser Asn Ser  Ser Asp Val 
    1025                 1030                 1035             


Val Gln  Gly Ile Gly Ala Arg  Thr Leu Thr Glu Phe  Lys Lys Phe 
    1040                 1045                 1050             


Asn Val  Asp Arg Phe Gly Arg  Lys His Glu Val Glu  Arg Glu Leu 
    1055                 1060                 1065             


Arg Thr  Trp Arg Gly Glu Thr  Trp Arg Gly Lys Ala  Tyr Ile 
    1070                 1075                 1080         


<210>  608
<211>  1084
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  608

Met Lys Asn Thr Leu Tyr Gly Ile Gly Leu Asp Ile Gly Val Ala Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Val Gly Leu Asn Gly Thr Gly Glu Pro Val Gly 
            20                  25                  30          


Leu His Arg Leu Gly Val Arg Ile Phe Asp Lys Ala Glu Gln Pro Lys 
        35                  40                  45              


Thr Gly Glu Ser Leu Ala Ala Pro Arg Arg Met Ala Arg Gly Met Arg 
    50                  55                  60                  


Arg Arg Leu Arg Arg Lys Ala Leu Arg Arg Ala Asp Val Tyr Ala Leu 
65                  70                  75                  80  


Leu Glu Arg Ser Gly Leu Ser Thr Arg Glu Ala Leu Ala His Met Phe 
                85                  90                  95      


Glu Ala Gly Gly Leu Glu Asp Ile Tyr Ala Leu Arg Thr Arg Ala Leu 
            100                 105                 110         


Asp Glu Pro Val Gly Lys Ala Glu Phe Ser Arg Ile Leu Leu His Leu 
        115                 120                 125             


Ala Gln Arg Arg Gly Phe Lys Ser Asn Arg Arg Thr Ala Ser Asp Gly 
    130                 135                 140                 


Glu Asp Gly Arg Leu Leu Ala Ala Val Asn Glu Asn Arg Arg Arg Met 
145                 150                 155                 160 


Ala Gln Gly Gly Trp Arg Thr Val Gly Glu Met Leu Tyr Arg His Glu 
                165                 170                 175     


Ala Phe Ala Leu His Lys Arg Asn Lys Ala Asp Glu Tyr Leu Ser Thr 
            180                 185                 190         


Val Gly Arg Asp Met Val Ala Glu Glu Ala Ser Leu Leu Phe Gln Arg 
        195                 200                 205             


Gln Arg Glu Leu Gly Cys Ala Trp Ala Thr Pro Glu Leu Gln Ala Glu 
    210                 215                 220                 


Tyr Leu Ser Ile Leu Leu Arg Gln Arg Ser Phe Asp Glu Gly Pro Gly 
225                 230                 235                 240 


Gly Asn Ser Pro Tyr Gly Gly Asn Gln Val Glu Lys Met Val Gly Arg 
                245                 250                 255     


Cys Thr Phe Glu Pro Asp Glu Pro Arg Ala Ala Lys Ala Ala Tyr Ser 
            260                 265                 270         


Phe Glu Tyr Phe Ser Leu Leu Gln Lys Ile Asn His Ile Arg Leu Val 
        275                 280                 285             


Glu Asn Gly Glu Thr Arg Pro Leu Thr Gln Gln Gln Arg Gln Gln Leu 
    290                 295                 300                 


Leu Ser Leu Ala His Lys Thr Pro Asp Val Ser Leu Ala Arg Ile Arg 
305                 310                 315                 320 


Lys Glu Leu Ala Leu Pro Glu Thr Val Gln Phe Asn Gly Val Arg Cys 
                325                 330                 335     


Arg Ala Asn Glu Thr Leu Glu Glu Ser Glu Lys Lys Glu Lys Phe Ala 
            340                 345                 350         


Cys Leu Pro Ala Tyr His Lys Met Arg Lys Ala Leu Asp Gly Val Val 
        355                 360                 365             


Lys Gly Arg Ile Ser Ser Leu Ser Ile Ser Gln Arg Asp Ala Ala Ala 
    370                 375                 380                 


Thr Ala Leu Ser Leu Tyr Lys Asn Glu Asp Thr Leu Arg Ala Lys Leu 
385                 390                 395                 400 


Thr Glu Ala Gly Phe Gln Ala Pro Glu Ile Asp Ala Leu Ala Gly Leu 
                405                 410                 415     


Thr Gly Phe Ser Lys Phe Gly His Leu Ser Leu Lys Ala Cys Arg Lys 
            420                 425                 430         


Leu Ile Pro His Leu Glu Gln Gly Leu Thr Tyr Asp Gln Ala Cys Ser 
        435                 440                 445             


Ala Ala Gly Tyr Asp Phe Lys Gly His Gly Ala Gly Glu Arg Ala Phe 
    450                 455                 460                 


Thr Leu Pro Ala Ala Ala Pro Glu Met Glu Gln Ile Thr Ser Pro Val 
465                 470                 475                 480 


Val Arg Arg Ala Val Ala Gln Thr Ile Lys Val Val Asn Gly Ile Ile 
                485                 490                 495     


Arg Glu Met Asp Ala Ser Pro Ala Trp Val Arg Ile Glu Leu Ala Arg 
            500                 505                 510         


Glu Leu Ser Lys Thr Phe Gly Glu Arg Gln Glu Met Asp Arg Ser Met 
        515                 520                 525             


Arg Glu Asn Ala Ala Gln Asn Glu Arg Leu Met Gln Glu Leu Arg Asp 
    530                 535                 540                 


Thr Phe His Leu Leu Ser Pro Thr Gly Gln Asp Leu Val Lys Tyr Arg 
545                 550                 555                 560 


Leu Trp Lys Glu Gln Asp Gly Val Cys Ala Tyr Ser Leu Arg Arg Leu 
                565                 570                 575     


Asp Val Glu Arg Leu Phe Glu Pro Gly Tyr Val Asp Val Asp His Ile 
            580                 585                 590         


Val Pro Tyr Ser Leu Ser Phe Asp Asp Arg Arg Ser Asn Lys Val Leu 
        595                 600                 605             


Val Leu Ser Ser Glu Asn Arg Gln Lys Gly Asn Arg Leu Pro Leu Gln 
    610                 615                 620                 


Tyr Leu Gln Gly Lys Arg Arg Glu Asp Phe Ile Val Trp Thr Asn Ser 
625                 630                 635                 640 


Ser Val Arg Asp Tyr Arg Lys Arg Gln Asn Leu Leu Arg Glu Lys Leu 
                645                 650                 655     


Ser Gly Asp Glu Ala Glu Gly Phe Arg Gln Arg Asn Leu Gln Asp Thr 
            660                 665                 670         


Gln His Met Ala Arg Phe Leu Tyr Asn Tyr Ile Ser Asp His Leu Ala 
        675                 680                 685             


Phe Ala Gln Ser Glu Ala Leu Gly Lys Lys Arg Val Phe Ala Val Ser 
    690                 695                 700                 


Gly Ala Val Thr Ser His Leu Arg Lys Arg Trp Gly Leu Ser Lys Val 
705                 710                 715                 720 


Arg Ala Asp Gly Asp Leu His His Ala Leu Asp Ala Ala Val Ile Ala 
                725                 730                 735     


Cys Thr Thr Asp Gly Met Ile Arg Arg Ile Ser Gly Tyr Tyr Gly His 
            740                 745                 750         


Ile Glu Gly Glu Tyr Leu Gln Asp Ala Asp Gly Ala Gly Ser Gln His 
        755                 760                 765             


Ala Arg Thr Lys Glu Arg Phe Pro Ala Pro Trp Pro Arg Phe Arg Asp 
    770                 775                 780                 


Glu Leu Ile Val Arg Leu Ser Glu Gln Pro Gly Glu His Leu Leu Asp 
785                 790                 795                 800 


Ile Asn Pro Glu Phe Tyr Cys Glu Tyr Gly Thr Glu His Ile Arg Pro 
                805                 810                 815     


Val Phe Val Ser Arg Met Pro Arg Arg Lys Val Thr Gly Pro Gly His 
            820                 825                 830         


Lys Glu Thr Ile Lys Gly Ala Ala Ala Ala Asp Glu Gly Leu Leu Thr 
        835                 840                 845             


Val Arg Lys Ala Leu Thr Glu Leu Lys Leu Asp Lys Asp Gly Glu Ile 
    850                 855                 860                 


Lys Asp Tyr Tyr Met Pro Ser Ser Asp Thr Leu Leu Tyr Glu Ala Leu 
865                 870                 875                 880 


Lys Ala Gln Leu Arg Arg Phe Gly Gly Asp Ser Lys Lys Ala Phe Ala 
                885                 890                 895     


Glu Pro Phe Tyr Lys Pro Lys Ala Asp Gly Thr Pro Gly Pro Leu Val 
            900                 905                 910         


Arg Lys Val Lys Thr Thr Glu Lys Ala Thr Leu Thr Val Pro Val His 
        915                 920                 925             


Gly Gly Ala Ala Ser Asn Asp Thr Met Val Arg Val Asp Val Phe Leu 
    930                 935                 940                 


Val Pro Gly Asp Gly Tyr Tyr Trp Val Pro Val Tyr Thr Ala Asp Thr 
945                 950                 955                 960 


Leu Lys Pro Glu Leu Pro Asn Arg Ala Val Val Gln Gly Lys Pro Tyr 
                965                 970                 975     


Ser Glu Trp Lys Glu Met Arg Glu Glu Asp Phe Ile Phe Ser Leu Tyr 
            980                 985                 990         


Pro Asn Asp Leu Val Tyr Val Glu  His Lys Ser Gly Leu  Lys Phe Thr 
        995                 1000                 1005             


Leu Gln  Asn Ala Asp Ser Thr  Leu Glu Lys Thr Arg  Thr Gln Thr 
    1010                 1015                 1020             


Ser Ala  Phe Val Tyr Phe Val  Gly Gly Ser Ile Ser  Thr Ala Ser 
    1025                 1030                 1035             


Ile Thr  Ile Arg Thr His Asp  Asn Ala Tyr Gly Ile  Pro Ser Leu 
    1040                 1045                 1050             


Gly Val  Lys Thr Leu Lys Thr  Phe His Lys Tyr Gln  Val Asp Val 
    1055                 1060                 1065             


Leu Gly  Asn Val Phe Pro Val  His Lys Glu Thr Arg  Gln Lys Phe 
    1070                 1075                 1080             


Arg 
    


<210>  609
<211>  1084
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  609

Met Lys Asn Thr Leu Tyr Gly Ile Gly Leu Asp Ile Gly Val Ala Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Val Gly Leu Asn Gly Thr Gly Glu Pro Val Gly 
            20                  25                  30          


Leu His Arg Leu Gly Val Arg Ile Phe Asp Lys Ala Glu Gln Pro Lys 
        35                  40                  45              


Thr Gly Glu Ser Leu Ala Ala Pro Arg Arg Met Ala Arg Gly Met Arg 
    50                  55                  60                  


Arg Arg Leu Arg Arg Lys Ala Leu Arg Arg Ala Asp Val Tyr Ala Leu 
65                  70                  75                  80  


Leu Glu Arg Ser Gly Leu Ser Thr Arg Glu Ala Leu Ala Gln Met Phe 
                85                  90                  95      


Glu Ala Gly Gly Leu Glu Asp Ile Tyr Ala Leu Arg Thr Arg Ala Leu 
            100                 105                 110         


Asp Glu Pro Val Gly Lys Ala Glu Phe Ser Arg Ile Leu Leu His Leu 
        115                 120                 125             


Ala Gln Arg Arg Gly Phe Lys Ser Asn Arg Arg Thr Ala Ser Asp Gly 
    130                 135                 140                 


Glu Asp Gly Arg Leu Leu Ala Ala Val Asn Glu Asn Arg Arg Arg Met 
145                 150                 155                 160 


Ala Gln Gly Gly Trp Arg Thr Val Gly Glu Met Leu Tyr Arg His Glu 
                165                 170                 175     


Ala Phe Ala Leu His Lys Arg Asn Lys Ala Asp Asp Tyr Leu Ser Thr 
            180                 185                 190         


Val Gly Arg Asp Met Val Ala Glu Glu Ala Ser Leu Leu Phe Gln Arg 
        195                 200                 205             


Gln Arg Glu Leu Gly Cys Ala Trp Ala Thr Pro Glu Leu Gln Ala Glu 
    210                 215                 220                 


Tyr Leu Ser Ile Leu Leu Arg Gln Arg Ser Phe Asp Glu Gly Pro Gly 
225                 230                 235                 240 


Gly Asp Ser Pro Tyr Gly Gly Asn Gln Val Glu Lys Met Val Gly Arg 
                245                 250                 255     


Cys Thr Phe Glu Pro Asp Glu Pro Arg Ala Ala Lys Ala Ala Tyr Ser 
            260                 265                 270         


Phe Glu Tyr Phe Ser Leu Leu Gln Lys Ile Asn His Ile Arg Leu Ala 
        275                 280                 285             


Glu Asn Gly Glu Thr Arg Pro Leu Thr Gln Pro Gln Arg Gln Gln Leu 
    290                 295                 300                 


Leu Ser Leu Ala His Lys Thr Pro Asp Met Ser Leu Ala Arg Ile Arg 
305                 310                 315                 320 


Lys Glu Leu Ala Leu Pro Glu Thr Val Gln Phe Asn Gly Val Arg Cys 
                325                 330                 335     


Arg Ala Asn Glu Thr Leu Glu Glu Ser Glu Lys Lys Glu Lys Phe Ala 
            340                 345                 350         


Cys Leu Pro Ala Tyr His Lys Met Arg Lys Ala Leu Gly Gly Val Val 
        355                 360                 365             


Lys Gly Arg Ile Ser Ser Leu Ser Ile Ser Gln Arg Asp Ala Ala Ala 
    370                 375                 380                 


Thr Ala Leu Ser Leu Tyr Lys Asn Glu Asp Thr Leu Arg Ala Lys Leu 
385                 390                 395                 400 


Thr Glu Ala Gly Phe Gln Ala Pro Glu Ile Asp Ala Leu Ala Gly Leu 
                405                 410                 415     


Thr Gly Phe Ser Lys Phe Gly His Leu Ser Leu Lys Ala Cys Arg Lys 
            420                 425                 430         


Leu Ile Pro His Leu Glu Gln Gly Leu Thr Tyr Asp Gln Ala Cys Ser 
        435                 440                 445             


Ala Ala Gly Tyr Asp Phe Lys Gly His Gly Ala Gly Glu Arg Ala Phe 
    450                 455                 460                 


Thr Leu Pro Ala Ala Ala Pro Glu Met Glu Gln Ile Thr Ser Pro Val 
465                 470                 475                 480 


Val Arg Arg Ala Val Ala Gln Thr Ile Lys Val Val Asn Gly Ile Ile 
                485                 490                 495     


Arg Glu Met Asp Ala Ser Pro Ala Trp Val Arg Ile Glu Leu Ala Arg 
            500                 505                 510         


Glu Leu Ser Lys Thr Phe Gly Glu Arg Gln Glu Met Asp Arg Ser Met 
        515                 520                 525             


Arg Glu Asn Ala Ala Lys Asn Glu Arg Leu Met Gln Glu Leu Arg Asp 
    530                 535                 540                 


Thr Phe His Leu Leu Ser Pro Thr Gly Gln Asp Leu Val Lys Tyr Arg 
545                 550                 555                 560 


Leu Trp Lys Glu Gln Asp Gly Val Cys Ala Tyr Ser Leu Gln Arg Leu 
                565                 570                 575     


Asp Val Glu Gln Leu Phe Glu Pro Gly Tyr Val Asp Val Asp His Ile 
            580                 585                 590         


Val Pro Tyr Ser Leu Ser Phe Asp Asp Arg Arg Ser Asn Lys Val Leu 
        595                 600                 605             


Val Leu Ser Ser Glu Asn Arg Gln Lys Gly Asn Arg Leu Pro Leu Gln 
    610                 615                 620                 


Tyr Leu Gln Gly Lys Arg Arg Glu Asp Phe Ile Val Trp Thr Asn Ser 
625                 630                 635                 640 


Ser Val His Asp Tyr Arg Lys Arg Gln Asn Leu Leu Arg Glu Lys Leu 
                645                 650                 655     


Ser Asp Asp Glu Ala Glu Gly Phe Arg Gln Arg Asn Leu Gln Asp Thr 
            660                 665                 670         


Gln His Met Ala Arg Phe Leu Tyr Asn Tyr Ile Ser Asp His Leu Ala 
        675                 680                 685             


Phe Ala Gln Ser Glu Ala Leu Gly Lys Lys Arg Val Phe Ala Val Ser 
    690                 695                 700                 


Gly Ala Val Thr Ser His Leu Arg Lys Arg Trp Gly Leu Ser Lys Val 
705                 710                 715                 720 


Arg Ala Asp Gly Asp Leu His His Ala Leu Asp Ala Ala Val Val Ala 
                725                 730                 735     


Cys Thr Thr Asp Gly Met Ile Arg Arg Ile Ser Gly Tyr Tyr Gly His 
            740                 745                 750         


Ile Glu Gly Glu Tyr Phe Gln Asp Ala Asp Gly Ala Gly Ser Gln His 
        755                 760                 765             


Ala Arg Thr Lys Glu Arg Phe Pro Ala Pro Trp Pro Arg Phe Arg Asp 
    770                 775                 780                 


Glu Leu Ile Val Arg Leu Ser Glu Gln Pro Gly Glu Pro Leu Leu Asp 
785                 790                 795                 800 


Ile Asn Pro Ala Phe Tyr Cys Glu Tyr Gly Thr Glu His Ile Arg Pro 
                805                 810                 815     


Val Phe Val Ser Arg Met Pro Arg Arg Lys Val Thr Gly Pro Ser His 
            820                 825                 830         


Lys Glu Thr Val Lys Gly Ala Ala Ala Ala Asp Glu Gly Leu Leu Thr 
        835                 840                 845             


Val Arg Lys Ala Leu Thr Glu Leu Lys Leu Asp Lys Asp Gly Glu Ile 
    850                 855                 860                 


Lys Asp Tyr Tyr Met Pro Ser Ser Asp Thr Leu Leu Tyr Glu Ala Leu 
865                 870                 875                 880 


Lys Ala Gln Leu Arg Arg Phe Gly Gly Asp Gly Lys Lys Ala Phe Ala 
                885                 890                 895     


Glu Pro Phe Tyr Lys Pro Lys Ala Asp Gly Thr Pro Gly Pro Leu Val 
            900                 905                 910         


Arg Lys Val Lys Thr Thr Glu Lys Ala Thr Leu Thr Val Pro Val His 
        915                 920                 925             


Gly Gly Ala Ala Ser Asn Asp Thr Met Val Arg Ile Asp Val Phe Phe 
    930                 935                 940                 


Met Pro Gly Asp Gly Tyr Tyr Trp Val Pro Leu Tyr Val Ala Asp Thr 
945                 950                 955                 960 


Leu Lys Pro Glu Leu Pro Asn Arg Ala Val Val Ala His Lys Pro Tyr 
                965                 970                 975     


Ser Glu Trp Gln Glu Met Arg Glu Glu Asp Phe Val Phe Ser Leu Tyr 
            980                 985                 990         


Pro Asn Asp Leu Val Tyr Val Glu  His Lys Ser Glu Leu  Lys Phe Thr 
        995                 1000                 1005             


Val Gln  Asn Ala Asp Ser Thr  Leu Glu Lys Thr Arg  Thr Gln Ala 
    1010                 1015                 1020             


Ser Ala  Phe Val Tyr Phe Val  Ser Gly Gly Ile Ser  Gly Gly Thr 
    1025                 1030                 1035             


Ile Thr  Val Arg Thr His Asp  Asn Ser Tyr Gly Ile  Pro Ser Leu 
    1040                 1045                 1050             


Gly Leu  Lys Thr Leu Lys Ser  Leu Cys Lys Tyr Gln  Val Asp Val 
    1055                 1060                 1065             


Leu Gly  Asn Val Phe Pro Val  His Lys Glu Thr Arg  Gln Lys Phe 
    1070                 1075                 1080             


Arg 
    


<210>  610
<211>  1084
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  610

Met Lys Asn Thr Leu Tyr Gly Ile Gly Leu Asp Ile Gly Val Ala Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Val Gly Leu Asn Gly Thr Gly Glu Pro Val Gly 
            20                  25                  30          


Leu His Arg Leu Gly Val Arg Ile Phe Asp Lys Ala Glu Gln Pro Lys 
        35                  40                  45              


Thr Gly Glu Ser Leu Ala Ala Pro Arg Arg Met Ala Arg Gly Met Arg 
    50                  55                  60                  


Arg Arg Leu Arg Arg Lys Ala Leu Arg Arg Ala Asp Val Tyr Ala Leu 
65                  70                  75                  80  


Leu Glu Arg Ser Gly Leu Ser Thr Arg Glu Glu Leu Ala Gln Met Phe 
                85                  90                  95      


Glu Ala Gly Gly Leu Glu Asp Ile Tyr Ala Leu Arg Thr Arg Ala Leu 
            100                 105                 110         


Asp Glu Pro Val Gly Lys Ala Glu Phe Ser Arg Ile Leu Leu His Leu 
        115                 120                 125             


Ala Gln Arg Arg Gly Phe Lys Ser Asn Arg Arg Thr Ala Ser Asp Gly 
    130                 135                 140                 


Glu Asp Gly Arg Leu Leu Ala Ala Val Asn Glu Asn Arg Arg Arg Met 
145                 150                 155                 160 


Ala Gln Gly Gly Trp Arg Thr Val Gly Glu Met Leu Tyr Arg His Glu 
                165                 170                 175     


Ala Phe Ala Leu His Lys Arg Asn Lys Ala Asp Asp Tyr Leu Ser Thr 
            180                 185                 190         


Val Gly Arg Asp Met Val Ala Glu Glu Ala Ser Ile Leu Phe Glu Arg 
        195                 200                 205             


Gln Arg Ala Met Gly Cys Ser Trp Ala Ala Pro Glu Leu Gln Ala Glu 
    210                 215                 220                 


Tyr Leu Ser Ile Leu Leu Arg Gln Arg Ser Phe Asp Glu Gly Pro Gly 
225                 230                 235                 240 


Gly Asn Ser Pro Tyr Gly Gly Asn Gln Val Glu Lys Met Val Gly Arg 
                245                 250                 255     


Cys Thr Phe Glu Pro Asp Glu Pro Arg Ala Ala Lys Ala Ala Tyr Ser 
            260                 265                 270         


Phe Glu Tyr Phe Ser Leu Leu Gln Lys Leu Asn His Ile Arg Leu Ala 
        275                 280                 285             


Glu Asn Gly Glu Thr Arg Pro Leu Thr Gln Pro Gln Arg Gln Gln Leu 
    290                 295                 300                 


Leu Ser Leu Ala His Lys Thr Pro Asp Ile Ser Leu Ala Arg Ile Arg 
305                 310                 315                 320 


Lys Glu Leu Ala Leu Pro Glu Thr Val Gln Phe Asn Gly Val Arg Cys 
                325                 330                 335     


Arg Ala Asn Glu Thr Leu Glu Glu Ser Glu Lys Lys Glu Lys Phe Ala 
            340                 345                 350         


Cys Leu Pro Ala Tyr His Lys Met Arg Lys Ala Leu Asp Gly Val Val 
        355                 360                 365             


Lys Gly Arg Ile Ser Ser Leu Ser Ile Ser Gln Arg Asp Ala Ala Ala 
    370                 375                 380                 


Thr Ala Leu Ser Leu Tyr Lys Asn Glu Asp Thr Leu Arg Ala Lys Leu 
385                 390                 395                 400 


Thr Glu Ala Gly Phe Gln Ala Pro Glu Ile Asp Ala Leu Ala Gly Leu 
                405                 410                 415     


Thr Gly Phe Ser Lys Phe Gly His Leu Ser Leu Lys Ala Cys Arg Lys 
            420                 425                 430         


Leu Ile Pro His Leu Glu Gln Gly Leu Thr Tyr Asp Gln Ala Cys Thr 
        435                 440                 445             


Ala Ala Gly Tyr Asp Phe Lys Gly His Gly Ala Gly Glu Arg Ala Phe 
    450                 455                 460                 


Thr Leu Pro Ala Ala Ala Pro Glu Met Glu Gln Ile Thr Ser Pro Val 
465                 470                 475                 480 


Val Arg Arg Ala Val Ala Gln Thr Ile Lys Val Val Asn Gly Ile Ile 
                485                 490                 495     


Arg Glu Met Asp Ala Ser Pro Ala Trp Val Arg Ile Glu Leu Ala Arg 
            500                 505                 510         


Glu Leu Ser Lys Thr Phe Gly Glu Arg Gln Glu Met Asp Arg Ser Met 
        515                 520                 525             


Arg Glu Asn Ala Ala Gln Asn Glu Arg Leu Met Gln Glu Leu Arg Asp 
    530                 535                 540                 


Thr Phe His Leu Leu Ser Pro Thr Gly Gln Asp Leu Val Lys Tyr Arg 
545                 550                 555                 560 


Leu Trp Lys Glu Gln Asp Gly Val Cys Ala Tyr Ser Leu Arg Arg Leu 
                565                 570                 575     


Asp Val Glu Arg Leu Phe Glu Pro Gly Tyr Val Asp Val Asp His Ile 
            580                 585                 590         


Val Pro Tyr Ser Leu Ser Phe Asp Asp Arg Arg Ser Asn Lys Val Leu 
        595                 600                 605             


Val Leu Ser Ser Glu Asn Arg Gln Lys Gly Asn Arg Leu Pro Leu Gln 
    610                 615                 620                 


Tyr Leu Gln Gly Lys Arg Arg Glu Asp Phe Ile Val Trp Thr Asn Ser 
625                 630                 635                 640 


Ser Val His Asp Tyr Arg Lys Arg Gln Asn Leu Leu Arg Glu Lys Leu 
                645                 650                 655     


Ser Gly Asp Glu Ala Glu Gly Phe Arg Gln Arg Asn Leu Gln Asp Thr 
            660                 665                 670         


Gln His Met Ala Arg Phe Leu Tyr Asn Tyr Ile Ser Asp His Leu Ala 
        675                 680                 685             


Phe Ala Gln Ser Glu Ala Leu Gly Lys Lys Arg Val Phe Ala Val Ser 
    690                 695                 700                 


Gly Ala Val Thr Ser His Leu Arg Lys Arg Trp Gly Leu Ser Lys Val 
705                 710                 715                 720 


Arg Ala Asp Gly Asp Leu His His Ala Leu Asp Ala Ala Val Val Ala 
                725                 730                 735     


Cys Thr Thr Asp Gly Met Ile Arg Arg Ile Ser Gly Tyr Tyr Gly His 
            740                 745                 750         


Ile Glu Gly Glu Tyr Leu Gln Asp Ala Asp Gly Ala Gly Ser Gln His 
        755                 760                 765             


Ala Arg Thr Lys Glu Arg Phe Pro Ala Pro Trp Pro Arg Phe Arg Asp 
    770                 775                 780                 


Glu Leu Ile Val Arg Leu Ser Glu Gln Pro Ala Glu Pro Leu Leu Asp 
785                 790                 795                 800 


Ile Asn Pro Glu Phe Tyr Cys Glu Tyr Gly Thr Glu His Ile Arg Pro 
                805                 810                 815     


Val Phe Val Ser Arg Met Pro Arg Arg Lys Val Thr Gly Pro Gly His 
            820                 825                 830         


Lys Glu Thr Ile Lys Gly Ala Ala Ala Ala Asp Glu Gly Leu Leu Thr 
        835                 840                 845             


Val Arg Lys Ala Leu Thr Glu Leu Lys Leu Asp Lys Asp Gly Glu Ile 
    850                 855                 860                 


Lys Asp Tyr Tyr Met Pro Ser Ser Asp Thr Leu Leu Tyr Glu Ala Leu 
865                 870                 875                 880 


Lys Ala Gln Leu Arg Arg Phe Gly Gly Asp Gly Lys Lys Ala Phe Ala 
                885                 890                 895     


Glu Pro Phe Tyr Lys Pro Lys Ala Asp Gly Thr Pro Gly Pro Leu Val 
            900                 905                 910         


Arg Lys Val Lys Thr Thr Glu Lys Ala Thr Leu Thr Val Pro Val His 
        915                 920                 925             


Gly Gly Ala Ala Ser Asn Asp Thr Met Val Arg Val Asp Val Phe Leu 
    930                 935                 940                 


Val Pro Gly Asp Gly Tyr Tyr Trp Val Pro Val Tyr Thr Ala Asp Thr 
945                 950                 955                 960 


Leu Lys Pro Glu Leu Pro Asn Arg Ala Val Val Gln Gly Lys Pro Tyr 
                965                 970                 975     


Ser Glu Trp Lys Glu Met Arg Glu Glu Asp Phe Ile Phe Ser Leu Tyr 
            980                 985                 990         


Pro Asn Asp Leu Val Tyr Val Glu  His Lys Ser Gly Leu  Lys Phe Thr 
        995                 1000                 1005             


Leu Gln  Asn Ala Asp Ser Thr  Leu Glu Lys Thr Arg  Thr Gln Thr 
    1010                 1015                 1020             


Ser Ala  Phe Ala Tyr Phe Val  Ser Gly Ser Ile Ser  Thr Ala Ser 
    1025                 1030                 1035             


Ile Thr  Ile Arg Thr His Asp  Asn Ala Tyr Gly Ile  Pro Ser Leu 
    1040                 1045                 1050             


Gly Val  Lys Thr Leu Lys Thr  Phe His Lys Tyr Gln  Val Asp Val 
    1055                 1060                 1065             


Leu Gly  Asn Val Phe Pro Val  His Lys Glu Thr Arg  Gln Lys Phe 
    1070                 1075                 1080             


Arg 
    


<210>  611
<211>  1084
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  611

Met Arg Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly Trp Trp Leu 
1               5                   10                  15      


Tyr Gly Thr Glu Gly Gly Arg Ile Thr Glu Val Val Asp Gly Gly Val 
            20                  25                  30          


Arg Ile Phe Ser Asp Gly Arg Asp Pro Gln Ser Lys Ala Ser Leu Ala 
        35                  40                  45              


Val Asp Arg Arg Ala Ala Arg Ser Gln Arg Arg Arg Arg Asp Arg Tyr 
    50                  55                  60                  


Leu Arg Arg Lys Ala Ala Leu Met Lys Arg Met Ala Gln Val Gly Leu 
65                  70                  75                  80  


Met Pro Ala Asp Pro Ala Glu Ala Lys Val Leu Glu Val Leu Asn Pro 
                85                  90                  95      


Tyr Glu Leu Arg Ala Ser Gly Leu Asp Gly Pro Leu Pro Leu Thr His 
            100                 105                 110         


Phe Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly Phe Lys Ser 
        115                 120                 125             


Asn Arg Lys Thr Asp Arg Gly Asp Asn Glu Ser Gly Lys Ile Lys Asp 
    130                 135                 140                 


Ala Thr Ala Arg Leu Asp Gln Ala Met Met Ala Lys Gly Ala Arg Thr 
145                 150                 155                 160 


Tyr Gly Glu Phe Leu His Met Arg Arg Ala Ala Ala Leu Asp Pro Lys 
                165                 170                 175     


Ser Val Pro Ser Val Arg Thr Arg Leu Thr Val Ala Arg Arg Asp Asp 
            180                 185                 190         


Ala Glu Lys Glu Glu Ala Gly Tyr Asp Phe Tyr Pro Asp Arg Lys His 
        195                 200                 205             


Leu Ser Glu Glu Phe Asp Lys Leu Trp Ala Ala Gln Ala Arg His Ala 
    210                 215                 220                 


Pro Asp Val Leu Thr Glu Asp Leu Arg Asp Glu Ile Ala Thr Ile Ile 
225                 230                 235                 240 


Phe His Gln Arg Pro Leu Lys Thr Pro Glu Val Gly Leu Cys Leu Phe 
                245                 250                 255     


Thr Gly Gln His Gly Val Pro Glu Arg Asp Lys Arg Ile Pro Ser Ala 
            260                 265                 270         


His Pro Leu Asn Gln Arg Arg Ile Leu Met Glu Thr Val Asn Asn Leu 
        275                 280                 285             


Lys Val Ala Ala Gln Gly Glu Pro Ser Arg Pro Leu Thr Arg Glu Glu 
    290                 295                 300                 


Arg Asp Gln Ile Val His Ala Leu Asp Asn Lys Lys His Thr Lys Ser 
305                 310                 315                 320 


Leu Ser Gly Met Thr Met Lys Leu Lys Ala Leu Gly Lys Val Ile Lys 
                325                 330                 335     


Leu Arg Pro Glu Gln Gly Phe Thr Leu Glu Thr Ala Asn Arg Asp Ala 
            340                 345                 350         


Ile Ala Cys Asp Pro Val Arg Ala Ser Leu Ser His Pro Asp Arg Phe 
        355                 360                 365             


Gly Ala Arg Trp Ser Thr Leu Glu Ser Ala Gly Gln Trp Asp Val Val 
    370                 375                 380                 


Gln Arg Ile Arg Ala Val Gln Ser Asp Ala Glu His Ala Asp Leu Val 
385                 390                 395                 400 


Gly Trp Leu Met Asp Val His Gly Leu Asp Arg Asp His Ala Glu Glu 
                405                 410                 415     


Thr Ala Asn Ala Pro Leu Pro Glu Gly Tyr Gly Arg Leu Gly Leu Thr 
            420                 425                 430         


Ala Thr Thr Arg Ile Leu Ala Ala Leu Glu Ala Asp Val Leu Asn Tyr 
        435                 440                 445             


Ser Ala Ala Val Ala Ala Cys Gly Trp His His Ser Asp Gly Arg Thr 
    450                 455                 460                 


Gly Glu Val Leu Glu Ser Leu Pro Tyr Tyr Gly Glu Ile Leu Glu Arg 
465                 470                 475                 480 


His Val Ile Pro Gly Thr Gly Asp Pro Asn Asp Asp Asp Val Lys Arg 
                485                 490                 495     


Tyr Gly Arg Ile Thr Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu 
            500                 505                 510         


Arg Arg Leu Val Asn Arg Ile Ile Thr Val Tyr Gly Arg Pro Asp Glu 
        515                 520                 525             


Ile Val Val Glu Leu Ala Arg Asp Leu Lys Leu Ser Glu Asp Gln Lys 
    530                 535                 540                 


Lys Asp Val Gln Arg Asp Ile Lys Lys Asn Thr Glu Ala Ala Ile Arg 
545                 550                 555                 560 


Arg Gly Lys Lys Leu Glu Glu Met Gly Val Pro Asn Thr Gly Ala Asn 
                565                 570                 575     


Arg Met Val Leu Arg Leu Trp Glu Asp Leu Gly Asp Asp Val Met Thr 
            580                 585                 590         


Arg Asn Cys Pro Tyr Thr Gly Lys Arg Ile Ser Ala Gly Met Leu Phe 
        595                 600                 605             


Asp Gly Ser Cys Asp Val Asp His Ile Leu Pro Tyr Ser Arg Thr Leu 
    610                 615                 620                 


Asp Asp Ser Phe Ala Asn Arg Thr Leu Cys Leu Lys Glu Ala Asn Arg 
625                 630                 635                 640 


Gln Lys Thr Asp Lys Thr Pro Trp Glu Ala Trp Gly Glu Thr Glu Gln 
                645                 650                 655     


Trp Glu Ala Ile Ala Ala Asn Leu Lys Asn Leu Pro Ala Asn Lys Ala 
            660                 665                 670         


Trp Arg Phe Ala Pro Asp Ala Met Glu Arg Phe Glu Gly Glu Lys Asp 
        675                 680                 685             


Phe Ser Ala Arg Ala Leu Ala Asp Thr Gln Tyr Leu Ser Arg Ile Ala 
    690                 695                 700                 


Arg Ala Tyr Leu Asp Ala Leu Tyr Asp Gly Asp Asp Gly Lys Ser His 
705                 710                 715                 720 


Val Trp Val Val Pro Gly Arg Leu Thr Glu Met Leu Arg Arg His Trp 
                725                 730                 735     


Gly Leu Asn Gly Val Ala Ala Leu Thr Asp Ser Asp Lys Gln Thr Val 
            740                 745                 750         


Lys Ala Lys Asn Arg Thr Asp His Arg His His Ala Ile Asp Ala Ala 
        755                 760                 765             


Val Val Ala Ala Thr Asp Arg Gly Leu Ile Lys Arg Ile Ala Asp Met 
    770                 775                 780                 


Ala Lys Arg Asp Glu Phe Asn Gly Ala Glu Glu Val Ala Arg Ser Ile 
785                 790                 795                 800 


Pro Pro Pro Trp Glu Gly Phe Arg Asp Asp Val Ser Ala Gln Val Thr 
                805                 810                 815     


Arg Ile Ile Val Ser His Arg Ala Asp His Gly Arg Ile Asp Pro Ala 
            820                 825                 830         


Ala Arg Lys Ser Gly Arg Asp Ser Thr Ser Gly Gln Leu His Asn Asp 
        835                 840                 845             


Thr Ala Tyr Gly Ile Gly Arg Asp Gly Thr Val Val Ser Arg Lys Pro 
    850                 855                 860                 


Leu Ala Ser Leu Ser Pro Asn Asp Ile Val Val Thr Lys Arg Gly Ala 
865                 870                 875                 880 


Asn Ile Arg Asp Val Asp Leu Gln Lys His Leu Ala Arg Val Thr His 
                885                 890                 895     


Gly Leu Asp Gly Lys Ala Phe Glu Ala Ala Leu Leu Ala Phe Ala Ala 
            900                 905                 910         


Ser Pro Ile Leu Pro Asp Asn Thr Asp Asn Pro Tyr Phe Gly Leu Arg 
        915                 920                 925             


Arg Val Arg Leu Glu Glu Thr Leu Gln Glu Ser Ala Arg Val Glu Val 
    930                 935                 940                 


Ala Asp Gln Glu Gly Arg Pro Tyr Lys Ala Tyr Lys Gly Asp Ser Asn 
945                 950                 955                 960 


His Cys Tyr Glu Ile Trp Cys Leu Pro Asp Gly Lys Ile Lys Pro Gln 
                965                 970                 975     


Val Val Thr Thr Tyr Glu Ala His Gln Ser Gly Ala Glu Lys Lys Pro 
            980                 985                 990         


His Pro Ala Ala Lys Arg Leu Met  Arg Ile Phe Lys Arg  Asp Met Val 
        995                 1000                 1005             


Met Leu  Glu Arg Asp Gly Glu  Thr Ile Ile Gly Tyr  Val Gln Lys 
    1010                 1015                 1020             


Met Lys  Gln Asn Gly Ser Ile  Phe Val Ala Pro His  Thr Glu Ala 
    1025                 1030                 1035             


Asn Ala  Asp Ala Arg Asp Arg  Asp Pro Lys Asp Asp  Phe Lys Leu 
    1040                 1045                 1050             


Ile Gln  Leu Gly Ala Gly Ser  Leu Val Lys Ala Lys  Ala Arg Arg 
    1055                 1060                 1065             


Val Ile  Val Asp Glu Met Gly  Arg Leu Arg Asp Pro  Gly Pro Pro 
    1070                 1075                 1080             


Leu 
    


<210>  612
<211>  1085
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  612

Met Asp Lys Gly Thr Asn Ser Ile Gly Trp Ala Ala Leu Arg Leu Asp 
1               5                   10                  15      


Glu Gln Gly Glu Pro Val Gly Ile Ile Glu Ala Gly Val Arg Ile Tyr 
            20                  25                  30          


Ala Asp Gly Arg Glu Pro Lys Ser Gly Thr Ser Leu Ala Glu Gly Arg 
        35                  40                  45              


Arg Ile Ala Arg Gly Met Ser Arg Arg Arg Asp Arg Tyr Lys Arg Arg 
    50                  55                  60                  


Arg Arg Thr Val Leu Asn Thr Leu Thr Glu Phe Gly Leu Met Pro Ala 
65                  70                  75                  80  


Thr Lys Ala Ala Arg Lys Ala Leu Val Ala Glu Thr Asn Asp Arg Arg 
                85                  90                  95      


Phe Gly Glu Val Ala Ser Asn Val Tyr Ala Leu Arg Ala Arg Ala Leu 
            100                 105                 110         


Asp Glu Lys Leu Pro Leu Tyr Gln Leu Gly Arg Ala Leu Phe His Leu 
        115                 120                 125             


Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Arg Asp 
    130                 135                 140                 


Asn Asp Gln Gly Lys Ile Ala Ser Ala Ile Ser Arg Leu Gly Arg Lys 
145                 150                 155                 160 


Leu Asp Glu Ala Gly Ala Arg Thr Leu Gly Glu Phe Leu His Lys Gln 
                165                 170                 175     


His Ala Ala Gly Lys Trp Val Arg Val Arg Pro Ser Arg Val Pro Gly 
            180                 185                 190         


Glu Asp Asp Lys Glu Glu Asp Gly Tyr Gly Phe Tyr Pro Glu Arg Ser 
        195                 200                 205             


Phe Leu Glu Arg Glu Phe Ala Ala Ile Trp Glu Ala Gln Ala Ala Phe 
    210                 215                 220                 


Tyr Pro Glu Leu Leu Thr Glu Glu Arg Arg Asp His Leu His Trp Val 
225                 230                 235                 240 


Met Phe Phe Gln Arg Pro Leu Lys Arg Pro Thr Val Gly Lys Cys Ser 
                245                 250                 255     


Phe His Pro Asp Glu Asp Arg Leu Pro Lys Ala His Pro Leu Phe Gln 
            260                 265                 270         


Glu Phe Arg Leu Ile Lys Glu Leu Asn Glu Leu Glu Val Val His Arg 
        275                 280                 285             


Gly Asp Arg Arg Glu Lys Leu Thr Pro Asp Gln Arg Asp Arg Leu Phe 
    290                 295                 300                 


Asn Gln Leu Arg Thr Gln Arg Ser Ser Ala Phe Thr Ala Leu Arg Lys 
305                 310                 315                 320 


Thr Ile Lys Ala Pro Thr Gly Thr Arg Phe Asn Lys Glu Ser Asp Ala 
                325                 330                 335     


Arg Asp Lys Leu Lys Gly Asp Glu Val Ala Ala Glu Met Ser His Lys 
            340                 345                 350         


Asp Arg Phe Gly Gln Ala Trp Ile Gly Met Pro Leu Ala Glu Arg Trp 
        355                 360                 365             


Ala Val Val Ala Met Leu Leu Asp Thr Glu Asp Pro Leu Glu Val Lys 
    370                 375                 380                 


Ala Trp Leu Glu Glu Arg Tyr Gly Phe Ala Ala Asp Arg Ile Asp Ala 
385                 390                 395                 400 


Ile Ala Ala Ala Lys Leu Pro Thr Gly Tyr Gly Arg Leu Gly Glu Thr 
                405                 410                 415     


Ala Leu Arg Asp Met Leu Asp Glu Leu Arg Asn Gly Thr Val Lys Asp 
            420                 425                 430         


Asp Ala Gly Arg Asp Val Val Leu Thr Glu Arg Gln Ala Met Asp Asn 
        435                 440                 445             


Ile Trp Gly Asp Asn Pro Ala Leu Gln Arg Ala Val Thr Asp Ala Arg 
    450                 455                 460                 


Glu Leu Pro Pro Tyr Gln Glu Val Leu Gly Arg Arg Ile Pro Pro Gly 
465                 470                 475                 480 


Thr Gly Lys Pro Ala Asp Pro Tyr Asp Val Gln Lys Gly Arg Leu Thr 
                485                 490                 495     


Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu Lys Arg Ile Val Asn 
            500                 505                 510         


Val Leu Met Arg Arg His Gly Arg Pro Glu Gln Ile Ala Leu Glu Leu 
        515                 520                 525             


Ala Arg Asp Leu Lys Leu Asn Glu Glu Gln Lys Ala Glu Lys Asn Arg 
    530                 535                 540                 


Glu Ile Ala Arg Asn Thr Lys Ala Ala Glu Ala Arg Gly Lys Lys Leu 
545                 550                 555                 560 


Glu Glu Leu Gly His Ala Asn Asn Gly Tyr Asn Arg Val Leu Leu Lys 
                565                 570                 575     


Leu Trp Glu Asp Leu Asn Leu Lys Gln Pro Glu Asn Arg Glu Cys Ile 
            580                 585                 590         


Tyr Cys Gly Lys Ala Ile Gly Ile Gly Thr Leu Leu Ser Asn Gln Val 
        595                 600                 605             


Asp Ile Asp His Ile Leu Pro Trp Ser Lys Thr Leu Asp Asp Ser Gln 
    610                 615                 620                 


Ala Asn Arg Ile Val Cys His Val Glu Cys Asn Arg Gln Lys Gly Asn 
625                 630                 635                 640 


His Ala Pro Ala Glu Val Pro Gln Trp His Asp Arg Tyr Asp Asp Ile 
                645                 650                 655     


Leu Ala Arg Ala Glu Arg Leu Pro Arg Asn Lys Ala Trp Arg Phe Gly 
            660                 665                 670         


Arg Glu Ala Met Lys Lys Phe Lys Asp Asp Glu Ser Phe Leu Ala Arg 
        675                 680                 685             


Gln Leu Thr Asp Thr Gln Tyr Leu Ser Arg Leu Ala His Glu Tyr Leu 
    690                 695                 700                 


Gly Ala Leu Tyr Pro Asp Glu Glu Pro Asp Glu Trp Gly Gly Pro Ala 
705                 710                 715                 720 


Arg Arg Asn His Val Arg Val Ile Pro Gly Arg Met Thr Glu Met Leu 
                725                 730                 735     


Arg Arg Gln Trp Gly Leu Asn Ala Ile Ile Ser Asp His Asn Gln Lys 
            740                 745                 750         


Asn Arg Asn Asp His Arg His His Ala Ile Asp Ala Ile Val Val Gly 
        755                 760                 765             


Val Thr Thr Arg Arg Leu Leu Gln Arg Ile Ala Thr Val Ala Gly Gly 
    770                 775                 780                 


Arg Glu Ala Leu Ala Leu Glu Glu Thr Ile Gly Lys Ile Glu Pro Pro 
785                 790                 795                 800 


Trp Pro Gly Phe Tyr Asp Asp Val Lys Ala Ala Ile Asp Arg Ile Val 
                805                 810                 815     


Val Ser His Lys Pro Asp His Gly Ser Leu Pro Lys Pro Gly Lys Pro 
            820                 825                 830         


Gly Arg Thr Ala Gly Gln Leu His Asn Asp Thr Ala Tyr Gly Phe Thr 
        835                 840                 845             


Gly Glu Ala Arg Asn Gly Val Pro Ile Met Val His Arg Val Pro Phe 
    850                 855                 860                 


Ala Ser Leu Thr Glu Lys Asp Ile Pro Ser Ile Arg Asp Arg Gln Leu 
865                 870                 875                 880 


Ala Asp Ala Leu Tyr Ala Ala Ile Ser Gly Ile Ser Gly Lys Ala Glu 
                885                 890                 895     


Leu Ala Ala Ala Leu Ala Arg Phe Ser Glu Ser Gly Pro Tyr Lys Gly 
            900                 905                 910         


Ile Arg His Val Arg Val Val Glu Pro Val Ser Val Ile Ala Ile Lys 
        915                 920                 925             


Asp Gln Ala Gly Lys Ala Tyr Lys Gly Tyr Lys Gly Asp Ala Asn Tyr 
    930                 935                 940                 


Arg Tyr Asp Val Trp Glu Leu Pro Asn Gly Lys Trp Thr His Glu Val 
945                 950                 955                 960 


Val Ser Met Phe Asn Ala His Arg Pro Asp Trp Gln Ser Glu Ile Arg 
                965                 970                 975     


Ser Lys Asn Pro Ala Ala Arg Lys Val Leu Ser Leu His Gln Asn Asp 
            980                 985                 990         


Met Val Ala Tyr Glu His Pro Gln  Asp Gly Tyr Thr Ile  Gly Arg Val 
        995                 1000                 1005             


Val Lys  Phe Ser Ala Gly Gly  Gln Met Ala Leu Ala  Gly Asn Arg 
    1010                 1015                 1020             


Glu Gly  Gly Ala Leu Lys Ser  Arg Asp Ala Asp Pro  Glu Asp Arg 
    1025                 1030                 1035             


Phe Arg  Tyr Phe Tyr Lys Ser  Gly Gly Ala Met Arg  Asp Val Gln 
    1040                 1045                 1050             


Leu Arg  Gln Val Arg Val Asp  Glu Thr Gly Arg Val  Phe Asp Pro 
    1055                 1060                 1065             


Gly Pro  Gln Asp Arg Glu Ser  Arg Asn Ala Arg Lys  Gln Asn Asp 
    1070                 1075                 1080             


Ala Gly  
    1085 


<210>  613
<211>  1087
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  613

Met Arg Tyr Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Met Asn Leu Asp Ile Pro Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Lys 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Val Ile Arg Glu Gly Ile Leu 
65                  70                  75                  80  


Thr Lys Glu Glu Leu Asp Lys Leu Phe Glu Glu Lys His Glu Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ser Glu Arg Ser Asn Lys Glu Asn Ser Thr Met Leu 
    130                 135                 140                 


Lys His Ile Glu Glu Asn Arg Ala Ile Leu Ser Ser Tyr Arg Thr Val 
145                 150                 155                 160 


Gly Glu Met Ile Val Lys Asp Pro Lys Phe Ala Leu His Lys Arg Asn 
                165                 170                 175     


Lys Gly Glu Asn Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu Glu Arg 
            180                 185                 190         


Glu Ile Arg Leu Ile Phe Ser Lys Gln Arg Glu Phe Gly Asn Met Ser 
        195                 200                 205             


Cys Thr Glu Glu Phe Glu Asn Glu Tyr Ile Thr Ile Trp Ala Ser Gln 
    210                 215                 220                 


Arg Pro Val Ala Ser Lys Asp Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Ile Ala Trp Glu His Ile Asn Lys Leu Arg Leu Ile Ser 
            260                 265                 270         


Pro Ser Gly Ala Arg Gly Leu Thr Asp Glu Glu Arg Arg Leu Leu Tyr 
        275                 280                 285             


Glu Gln Ala Phe Gln Lys Asn Lys Ile Thr Tyr His Asp Ile Arg Thr 
    290                 295                 300                 


Leu Leu His Leu Pro Asp Asp Thr Tyr Phe Lys Gly Ile Val Tyr Asp 
305                 310                 315                 320 


Arg Gly Glu Ser Arg Lys Gln Asn Glu Asn Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ala Tyr His Gln Ile Arg Lys Ala Val Asp Lys Val Tyr Gly Lys 
            340                 345                 350         


Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Leu Phe Lys Asp Asp Ala Asp Ile His Ser Tyr Leu Arg 
    370                 375                 380                 


Asn Glu Tyr Glu Gln Asn Gly Lys Arg Met Pro Asn Leu Ala Asn Lys 
385                 390                 395                 400 


Val Tyr Asp Asn Glu Leu Ile Glu Glu Leu Leu Asn Leu Ser Phe Thr 
                405                 410                 415     


Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg Ser Ile Leu Pro Tyr 
            420                 425                 430         


Met Glu Gln Gly Glu Val Tyr Ser Ser Ala Cys Glu Arg Ala Gly Tyr 
        435                 440                 445             


Thr Phe Thr Gly Pro Lys Lys Lys Gln Lys Thr Met Leu Leu Pro Asn 
    450                 455                 460                 


Ile Pro Pro Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val Ser 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp Glu Arg 
            500                 505                 510         


Arg Lys Thr Lys Lys Glu Gln Asp Glu Asn Arg Lys Lys Asn Glu Thr 
        515                 520                 525             


Ala Ile Arg Gln Leu Met Glu Tyr Gly Leu Thr Leu Asn Pro Thr Gly 
    530                 535                 540                 


His Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly Arg Cys 
545                 550                 555                 560 


Ala Tyr Ser Leu Gln Pro Ile Glu Ile Glu Arg Leu Leu Glu Pro Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Arg Glu Asn Arg Glu Lys 
        595                 600                 605             


Gly Asn Arg Ile Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu Arg Trp 
    610                 615                 620                 


Gln Gln Phe Glu Thr Phe Val Leu Thr Asn Lys Gln Phe Ser Lys Lys 
625                 630                 635                 640 


Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu Glu Thr 
                645                 650                 655     


Glu Phe Lys Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Arg Phe 
            660                 665                 670         


Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Glu Ser Asp Asp 
        675                 680                 685             


Lys Gln Lys Val Tyr Thr Val Asn Gly Arg Val Thr Ala His Leu Arg 
    690                 695                 700                 


Ser Arg Trp Glu Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His His 
705                 710                 715                 720 


Ala Val Asp Ala Val Ile Val Ala Cys Thr Thr Pro Ser Asp Ile Ala 
                725                 730                 735     


Lys Val Thr Ala Phe Tyr Gln Arg Arg Glu Gln Asn Lys Glu Leu Ala 
            740                 745                 750         


Lys Lys Lys Glu Pro His Phe Pro Gln Pro Trp Pro His Phe Ala Asp 
        755                 760                 765             


Glu Leu Arg Ala Arg Leu Ser Lys His Pro Lys Glu Ser Ile Lys Ala 
    770                 775                 780                 


Leu Asn Leu Gly Asn Tyr Asp Asp Gln Lys Leu Glu Ser Leu Gln Pro 
785                 790                 795                 800 


Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala His 
                805                 810                 815     


Gln Glu Thr Leu Arg Arg Tyr Val Gly Ile Asp Glu Arg Ser Gly Lys 
            820                 825                 830         


Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu Asp Ala 
        835                 840                 845             


Ser Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Arg Thr Tyr 
    850                 855                 860                 


Glu Ala Ile Arg Gln Arg Leu Leu Glu His Asn Asn Asp Pro Lys Lys 
865                 870                 875                 880 


Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu Pro Gly 
                885                 890                 895     


Pro Val Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Gln Val Ile 
            900                 905                 910         


Pro Leu Asn Asp Gly Lys Thr Val Ala Tyr Asn Ser Asn Ile Val Arg 
        915                 920                 925             


Val Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro Val Tyr 
    930                 935                 940                 


Thr Met Asp Ile Met Lys Gly Ile Leu Pro Asn Lys Ala Ile Glu Pro 
945                 950                 955                 960 


Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Asp Tyr Thr Phe 
                965                 970                 975     


Arg Phe Ser Leu Tyr Pro Asn Asp Leu Ile Arg Ile Glu Leu Pro Arg 
            980                 985                 990         


Glu Lys Thr Val Lys Thr Ala Ala  Gly Glu Glu Ile Asn  Val Lys Asp 
        995                 1000                 1005             


Val Phe  Val Tyr Tyr Lys Thr  Ile Asp Ser Ala Asn  Gly Gly Leu 
    1010                 1015                 1020             


Glu Leu  Ile Ser His Asp His  Arg Phe Ser Leu Arg  Gly Val Gly 
    1025                 1030                 1035             


Ser Arg  Thr Leu Lys Arg Phe  Glu Lys Tyr Gln Val  Asp Val Leu 
    1040                 1045                 1050             


Gly Asn  Ile Tyr Lys Val Arg  Gly Glu Lys Arg Val  Gly Leu Ala 
    1055                 1060                 1065             


Ser Ser  Ala His Ser Lys Pro  Gly Lys Thr Ile Arg  Pro Leu Gln 
    1070                 1075                 1080             


Ser Thr  Arg Asp 
    1085         


<210>  614
<211>  1087
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  614

Met Leu Arg Met Lys Tyr Ala Ile Gly Leu Asp Ile Gly Ile Ala Ser 
1               5                   10                  15      


Val Gly Tyr Ala Val Leu Ala Leu Asp His Glu Glu Asn Pro Trp Gly 
            20                  25                  30          


Ile Ile Arg Leu Gly Ser Arg Ile Phe Asp Val Ala Glu Asn Pro Lys 
        35                  40                  45              


Asp Gly Ala Ser Leu Ala Leu Pro Arg Arg Glu Ala Arg Ser Val Arg 
    50                  55                  60                  


Arg Arg Leu Arg Arg His His His Arg Leu Glu Arg Ile Lys Asn Leu 
65                  70                  75                  80  


Leu Ile Asn Thr Glu Phe Ile Thr Lys Asp Glu Leu Leu His Leu Tyr 
                85                  90                  95      


Asp Gly Asn Leu Ser Asp Val Tyr Glu Leu Arg Val Lys Ala Leu Asp 
            100                 105                 110         


Tyr Ser Val Thr Asn Ala Glu Leu Thr Arg Ile Leu Leu His Leu Ala 
        115                 120                 125             


Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Ser Asp Ala Asn Asp Lys 
    130                 135                 140                 


Glu Ala Gly Gln Leu Leu Glu Ala Val Ser Ala Asn Ala Lys Arg Met 
145                 150                 155                 160 


Gln Glu Asn His Tyr Arg Thr Val Gly Glu Met Phe Tyr Lys Asp Asp 
                165                 170                 175     


Leu Phe Ser Lys Tyr Lys Arg Asn Lys Gly Gly Thr Tyr Leu Thr Thr 
            180                 185                 190         


Val His Arg Asp Met Ile Ala Ala Glu Ala Arg Thr Ile Leu Glu Lys 
        195                 200                 205             


Gln Phe Ala Leu Gly Asn Asn Ile Cys Thr Gln Asp Phe Ile Asp Lys 
    210                 215                 220                 


Tyr Leu Ser Ile Leu Leu Ser Gln Arg Gln Phe Asp Glu Gly Pro Gly 
225                 230                 235                 240 


Glu Pro Ser Pro Tyr Ala Gly Asn Gln Ile Ala Asn Met Ile Gly Lys 
                245                 250                 255     


Cys Thr Phe Glu Pro Asn Glu Tyr Arg Ala Ala Lys Ala Ser Tyr Ser 
            260                 265                 270         


Phe Glu Arg Phe Asn Leu Leu Gln Lys Val Asn His Leu Arg Leu Leu 
        275                 280                 285             


Leu Glu Gly Lys Ser Ile Ala Leu Asp Asn Glu Gln Arg Lys Lys Ile 
    290                 295                 300                 


Ile Ala Leu Ala His Asp Lys Ala Asp Leu Arg Tyr Ser His Ile Arg 
305                 310                 315                 320 


Lys Ala Leu Gly Leu Asp Glu Lys Val Leu Phe Asn Thr Ile Thr Tyr 
                325                 330                 335     


Asn Asp Asp Val Ser Val Ile Glu Lys Lys Thr Lys Phe Asn Phe Leu 
            340                 345                 350         


Gln Ala Tyr His Gln Ile Lys Lys Val Leu Ala Glu Asp Met Gln Lys 
        355                 360                 365             


Leu Thr Thr Glu Gln Leu Asp Asn Ile Gly Gln Ile Leu Ser Thr Tyr 
    370                 375                 380                 


Lys Ser Asp Asn Lys Arg Thr Glu Glu Leu Ser Ala Leu Gly Leu Glu 
385                 390                 395                 400 


Lys Lys Ile Ile Asp Ala Leu Leu Gly Ile Asn Gly Leu Ser Lys Phe 
                405                 410                 415     


Ala His Leu Ser Leu Lys Ala Leu Arg Lys Ile Asn Pro Tyr Leu Glu 
            420                 425                 430         


Glu Gly Lys Ile Tyr Asn Glu Ala Cys Thr Ala Ala Gly Tyr Asp Phe 
        435                 440                 445             


Lys Ala His Ala Asn Thr Gln Lys Thr Glu Leu Leu Pro Ala Tyr Lys 
    450                 455                 460                 


Glu Glu Met Asp Asp Ile Thr Ser Pro Val Ala Arg Arg Ala Ile Ala 
465                 470                 475                 480 


Gln Ser Ile Lys Val Ile Asn Ala Ile Ile Arg Glu Gln Lys Gly Ser 
                485                 490                 495     


Pro Leu Tyr Ile Asn Ile Glu Leu Ala Arg Glu Met Ala Lys Gly Phe 
            500                 505                 510         


Asp Glu Arg Thr Gln Ile Asp Lys Ala Asn Lys Glu Asn Gln Ala Lys 
        515                 520                 525             


Asn Glu Arg Ile Met Glu Arg Ile Arg Thr Glu Phe His Lys Ala Asn 
    530                 535                 540                 


Pro Thr Gly Met Asp Leu Ile Lys Leu Lys Leu Trp Glu Glu Gln Asp 
545                 550                 555                 560 


Gly Arg Ser Pro Tyr Ser Gln Lys Ala Ile Ser Ile Asn Arg Leu Phe 
                565                 570                 575     


Glu Pro Gly Tyr Val Asp Ile Asp His Ile Val Pro Tyr Ser Ile Ser 
            580                 585                 590         


Phe Asp Asp Ser Phe Lys Asn Lys Val Leu Val Phe Ser Asp Glu Asn 
        595                 600                 605             


Arg Asp Lys Gly Asn Arg Leu Pro Ile Ala Tyr Leu Gln Ser Lys Phe 
    610                 615                 620                 


Gly Ala Lys Ala Ala Glu Asn Phe Met Ile Trp Val Gln Ser Asn Ile 
625                 630                 635                 640 


Lys Asp Tyr Lys Lys Arg Gln Lys Leu Leu Lys Arg Glu Ile Thr Glu 
                645                 650                 655     


Glu Asp Ile Asn Lys Phe Lys Glu Arg Asn Leu Gln Asp Thr Lys Thr 
            660                 665                 670         


Ile Ser Arg Phe Leu Tyr Asn Tyr Ile Asn Asp Tyr Leu Leu Phe Ala 
        675                 680                 685             


Pro Ser Asp Thr Gly Lys Lys Lys Arg Val Thr Ala Val Asn Gly Thr 
    690                 695                 700                 


Ile Thr Ala Tyr Leu Arg Lys Arg Trp Gly Ile Asn Lys Ile Arg Ala 
705                 710                 715                 720 


Asn Gly Asp Lys His His Ala Val Asp Ala Val Val Ile Ala Cys Thr 
                725                 730                 735     


Thr Asp Lys Met Ile Lys Asp Leu Ser Ser Phe Ser Arg Tyr His Glu 
            740                 745                 750         


Leu Glu Tyr Thr His Thr Asp Thr Glu Ser Phe Leu Val Asn Ser Leu 
        755                 760                 765             


Thr Gly Glu Ile Leu Lys Arg Phe Pro Tyr Pro Trp Glu Asp Phe Arg 
    770                 775                 780                 


Pro Glu Leu Met Ala Arg Leu Ser Asp Asn Pro Ala Asp Ala Leu Arg 
785                 790                 795                 800 


Lys Leu Asn Leu Ile Phe Tyr His Gly Ile Asp Leu Ser Thr Ile Lys 
                805                 810                 815     


Pro Ile Phe Val Ser Arg Met Pro Arg His Lys Val Thr Gly Ala Ala 
            820                 825                 830         


His Lys Ala Thr Ile Lys Ser Ala Arg Cys Leu Asp Asn Gly Ile Val 
        835                 840                 845             


Ile Cys Lys Thr Ser Leu Gln Asn Leu Lys Leu Asp Lys Glu Gly Glu 
    850                 855                 860                 


Ile Ala Asn Tyr Tyr Ala Pro Glu Ser Asp Thr Ile Leu Tyr Asn Ala 
865                 870                 875                 880 


Leu Lys Glu Arg Leu Arg Ser Tyr Asp Asn Lys Pro Ala Lys Ala Phe 
                885                 890                 895     


Ala Glu Pro Phe Tyr Lys Pro Lys Ala Asp Gly Thr Pro Gly Pro Leu 
            900                 905                 910         


Val Lys Lys Val Lys Val Tyr Glu Lys Ser Thr Leu Asn Val Ala Val 
        915                 920                 925             


Gln Gln Asn Thr Ala Val Ala Asp Asn Asp Ser Met Val Arg Val Asp 
    930                 935                 940                 


Val Phe His Val Lys Gly Asn Gly Tyr Tyr Leu Val Pro Ile Tyr Ile 
945                 950                 955                 960 


Ala Asp Thr Leu Lys Lys Glu Leu Pro Asn Lys Ala Ile Val Ala Tyr 
                965                 970                 975     


Lys Pro Tyr Ala Asp Trp Pro Val Met Asp Asp Ser Asn Phe Ile Phe 
            980                 985                 990         


Ser Leu Tyr Pro Asn Asp Leu Ile  Lys Phe Glu His Lys  Asn Ser Val 
        995                 1000                 1005             


Lys Phe  Ala Lys Val Asn Lys  Glu Ser Thr Leu Asn  Asp Phe Tyr 
    1010                 1015                 1020             


Ser Thr  Lys Ser Glu Ile Val  Tyr Phe Lys Gly Cys  Asn Ile Asn 
    1025                 1030                 1035             


Thr Gly  Ala Ile Thr Ile Ile  Asn His Glu Asn Thr  Tyr Val Val 
    1040                 1045                 1050             


Ser Ser  Leu Gly Val Lys Thr  Leu Pro Ser Leu Glu  Lys Tyr Gln 
    1055                 1060                 1065             


Ala Asp  Val Leu Gly Asn Tyr  Thr Lys Val Lys Lys  Glu Val Arg 
    1070                 1075                 1080             


Lys Pro  Phe Arg 
    1085         


<210>  615
<211>  1087
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  615

Met Arg Tyr Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Leu Asp Ile Pro Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Lys 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Ile Ile Arg Glu Gly Ile Leu 
65                  70                  75                  80  


Thr Lys Glu Glu Leu Asp Lys Leu Phe Glu Glu Lys His Glu Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ser Glu Arg Ser Asn Lys Glu Asn Ser Thr Met Leu 
    130                 135                 140                 


Lys His Ile Glu Glu Asn Arg Ala Ile Leu Ser Ser Tyr Arg Thr Val 
145                 150                 155                 160 


Gly Glu Met Ile Val Lys Asp Pro Lys Phe Ala Leu His Lys Arg Asn 
                165                 170                 175     


Lys Gly Glu Asn Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu Glu Arg 
            180                 185                 190         


Glu Ile Arg Leu Ile Phe Ser Lys Gln Arg Glu Phe Gly Asn Met Ser 
        195                 200                 205             


Cys Thr Glu Glu Phe Glu Asn Glu Tyr Ile Ala Ile Trp Ala Ser Gln 
    210                 215                 220                 


Arg Pro Val Ala Ser Lys Asp Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Ile Ala Trp Glu His Ile Asn Lys Leu Arg Leu Ile Ser 
            260                 265                 270         


Pro Ser Gly Thr Arg Gly Leu Thr Asp Glu Glu Arg Arg Leu Leu Tyr 
        275                 280                 285             


Glu Gln Ala Phe Gln Lys Asn Lys Ile Thr Tyr His Asp Ile Arg Thr 
    290                 295                 300                 


Leu Leu His Leu Pro Asp Asp Thr Tyr Phe Lys Gly Ile Val Tyr Asp 
305                 310                 315                 320 


Arg Gly Glu Ser Arg Lys Gln Asn Glu Asn Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ala Tyr His Gln Ile Arg Lys Ala Val Asp Lys Val Tyr Gly Lys 
            340                 345                 350         


Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Leu Phe Lys Asp Asp Ala Asp Ile Arg Ser Tyr Leu Arg 
    370                 375                 380                 


Asn Glu Tyr Glu Gln Asn Gly Lys Arg Met Pro Asn Leu Ala Asn Lys 
385                 390                 395                 400 


Val Tyr Asp Asn Glu Leu Ile Glu Glu Leu Leu Asn Leu Ser Phe Thr 
                405                 410                 415     


Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg Ser Ile Leu Pro Tyr 
            420                 425                 430         


Met Glu Gln Gly Glu Val Tyr Ser Ser Ala Cys Glu Arg Ala Gly Tyr 
        435                 440                 445             


Thr Phe Thr Gly Pro Lys Lys Lys Gln Lys Thr Met Leu Leu Pro Asn 
    450                 455                 460                 


Ile Pro Pro Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val Ser 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp Glu Arg 
            500                 505                 510         


Arg Lys Thr Lys Lys Glu Gln Asp Glu Asn Arg Lys Lys Asn Glu Thr 
        515                 520                 525             


Ala Ile Arg Gln Leu Met Glu Tyr Gly Leu Thr Leu Asn Pro Thr Gly 
    530                 535                 540                 


His Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly Arg Cys 
545                 550                 555                 560 


Ala Tyr Ser Leu Gln Pro Ile Glu Ile Glu Arg Leu Leu Glu Pro Gly 
                565                 570                 575     


Tyr Thr Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Lys Glu Asn Arg Glu Lys 
        595                 600                 605             


Gly Asn Arg Ile Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu Arg Trp 
    610                 615                 620                 


Gln Gln Phe Glu Thr Phe Val Leu Thr Asn Lys Gln Phe Ser Lys Lys 
625                 630                 635                 640 


Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu Glu Thr 
                645                 650                 655     


Glu Phe Lys Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Arg Phe 
            660                 665                 670         


Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Glu Ser Asp Asp 
        675                 680                 685             


Lys Gln Lys Val Tyr Thr Val Asn Gly Arg Val Thr Ala His Leu Arg 
    690                 695                 700                 


Ser Arg Trp Glu Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His His 
705                 710                 715                 720 


Ala Val Asp Ala Val Ile Val Ala Cys Thr Thr Pro Ser Asp Ile Ala 
                725                 730                 735     


Lys Val Thr Ala Phe Tyr Gln Arg Arg Glu Gln Asn Lys Glu Leu Ala 
            740                 745                 750         


Lys Lys Thr Glu Pro His Phe Pro Gln Pro Trp Pro His Phe Ala Asp 
        755                 760                 765             


Glu Leu Arg Ala Arg Leu Ser Lys His Pro Lys Glu Ser Ile Lys Ala 
    770                 775                 780                 


Leu Asn Leu Gly Asn Tyr Asp Asp Gln Lys Leu Glu Ser Leu Gln Pro 
785                 790                 795                 800 


Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala His 
                805                 810                 815     


Gln Glu Thr Leu Arg Arg Tyr Val Gly Ile Asp Glu Arg Ser Gly Lys 
            820                 825                 830         


Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu Asp Ala 
        835                 840                 845             


Ser Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Arg Thr Tyr 
    850                 855                 860                 


Glu Ala Ile Arg Gln Arg Leu Leu Glu His Asn Asn Asp Pro Lys Lys 
865                 870                 875                 880 


Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu Pro Gly 
                885                 890                 895     


Pro Val Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Gln Val Ile 
            900                 905                 910         


Pro Leu Asn Asp Gly Lys Thr Val Ala Tyr Asn Ser Asn Ile Val Arg 
        915                 920                 925             


Val Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro Val Tyr 
    930                 935                 940                 


Thr Met Asp Ile Met Lys Gly Ile Leu Pro Asn Lys Ala Ile Glu Pro 
945                 950                 955                 960 


Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Asp Tyr Thr Phe 
                965                 970                 975     


Arg Phe Ser Leu Tyr Pro Asn Asp Leu Ile Arg Ile Glu Leu Pro Arg 
            980                 985                 990         


Glu Lys Ile Ile Lys Thr Ala Gly  Gly Glu Glu Ile Lys  Ile Lys Asp 
        995                 1000                 1005             


Leu Phe  Ala Tyr Tyr Lys Thr  Ile His Ser Gly Thr  Ala Gly Leu 
    1010                 1015                 1020             


Glu Leu  Val Ser His Asp Cys  Ser Phe Ser Leu Ser  Gly Val Gly 
    1025                 1030                 1035             


Ser Arg  Thr Leu Lys Arg Phe  Glu Lys Tyr Gln Val  Asp Val Leu 
    1040                 1045                 1050             


Gly Asn  Ile Tyr Lys Val Arg  Gly Glu Lys Arg Val  Gly Leu Ala 
    1055                 1060                 1065             


Ser Ser  Ala His Ser Lys Thr  Gly Glu Thr Ile Arg  Pro Leu Gln 
    1070                 1075                 1080             


Ser Thr  Arg Asp 
    1085         


<210>  616
<211>  1087
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  616

Met Arg Tyr Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Leu Asp Leu Pro Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Arg 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Leu Thr Arg Glu Asn Val Leu 
65                  70                  75                  80  


Thr Gln Glu Glu Leu Asp Lys Leu Phe Glu Lys Lys His Glu Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ser Glu Arg Ser Asn Lys Glu Asn Ser Thr Met Leu 
    130                 135                 140                 


Lys His Ile Glu Glu Asn Arg Ser Ile Leu Thr Gly Tyr Arg Thr Val 
145                 150                 155                 160 


Gly Glu Met Ile Val Arg Asp Pro Lys Phe Val Leu His Lys Arg Asn 
                165                 170                 175     


Lys Gly Glu Asn Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu Glu Arg 
            180                 185                 190         


Glu Ile Gln Leu Ile Phe Ala Arg Gln Arg Glu Phe Gly Asn Met Ile 
        195                 200                 205             


Cys Thr Glu Ala Phe Glu Asn Glu Tyr Ile Ala Ile Trp Ser Ser Gln 
    210                 215                 220                 


Arg Pro Val Ala Ser Lys Asp Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Ile Ala Trp Glu His Ile Asn Lys Leu Arg Leu Val Ser 
            260                 265                 270         


Pro Ser Gly Thr Arg Glu Leu Thr Asp Glu Glu Arg Arg Leu Leu Tyr 
        275                 280                 285             


Glu Gln Ala Phe Gln Lys Asn Lys Leu Thr Tyr His Asp Ile Arg Thr 
    290                 295                 300                 


Leu Leu His Leu Pro Asp Asp Thr Tyr Phe Lys Gly Ile Val Tyr Asp 
305                 310                 315                 320 


Trp Gly Glu Pro Leu Lys Gln Asn Glu Asn Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ala Tyr His Gln Ile Arg Lys Thr Val Asp Lys Val Tyr Gly Arg 
            340                 345                 350         


Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Leu Phe Lys Asp Asp Ala His Ile Arg Ser Tyr Leu Arg 
    370                 375                 380                 


Asn Glu Tyr Glu Gln Asn Gly Lys Arg Ile Ala Asn Leu Ala Asn Lys 
385                 390                 395                 400 


Val Tyr Asp Asn Glu Leu Ile Glu Glu Leu Leu Asn Leu Ser Phe Thr 
                405                 410                 415     


Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg Asn Ile Leu Pro Tyr 
            420                 425                 430         


Met Glu Gln Gly Glu Val Tyr Ser Ser Ala Cys Glu Arg Ala Gly Tyr 
        435                 440                 445             


Thr Phe Thr Gly Pro Thr Lys Lys Gln Lys Thr Val Leu Leu Pro Thr 
    450                 455                 460                 


Ile Pro Pro Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val Ser 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp Glu Arg 
            500                 505                 510         


Lys Lys Thr Lys Lys Glu Gln Asp Glu Asn Arg Lys Arg Asn Glu Thr 
        515                 520                 525             


Ala Ile Arg Gln Leu Ile Glu Tyr Gly Leu Thr Arg Asn Pro Thr Gly 
    530                 535                 540                 


His Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly Arg Cys 
545                 550                 555                 560 


Ala Tyr Ser Leu Gln Pro Ile Glu Ile Lys Arg Leu Leu Glu Pro Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Lys Glu Asn Arg Glu Lys 
        595                 600                 605             


Gly Asn Arg Ile Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu Arg Trp 
    610                 615                 620                 


Gln Gln Phe Glu Thr Phe Val Leu Thr Asn Lys Gln Phe Ser Lys Lys 
625                 630                 635                 640 


Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu Glu Ala 
                645                 650                 655     


Glu Trp Lys Asn Arg Ser Leu Asn Asp Thr Arg Tyr Ile Ser Arg Phe 
            660                 665                 670         


Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Asp Ser Asp Asp 
        675                 680                 685             


Lys Gln Lys Val Tyr Thr Val Asn Gly Gly Val Thr Ala His Leu Arg 
    690                 695                 700                 


Ser Arg Trp Asp Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His His 
705                 710                 715                 720 


Ala Val Asp Ala Ala Ile Val Ala Cys Thr Thr Leu Ser Asp Ile Ala 
                725                 730                 735     


Arg Val Thr Ala Phe Tyr Gln Arg Arg Glu Gln Ala Lys Glu Leu Ala 
            740                 745                 750         


Lys Lys Thr Glu Pro His Phe Pro Gln Pro Trp Pro His Phe Ala Asp 
        755                 760                 765             


Glu Leu Arg Ala Arg Leu Ser Lys Asn Pro Lys Glu Ser Ile Lys Ala 
    770                 775                 780                 


Leu Asn Leu Gly Asn Tyr Asp Asn Glu Lys Leu Glu Ser Leu Gln Pro 
785                 790                 795                 800 


Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala His 
                805                 810                 815     


Gln Glu Thr Leu Arg Arg Tyr Ile Gly Ile Asp Glu Arg Ser Gly Lys 
            820                 825                 830         


Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu Asp Ala 
        835                 840                 845             


Ser Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Arg Thr Tyr 
    850                 855                 860                 


Glu Ala Ile Arg Leu Arg Leu Leu Glu His Asn Asn Asp Pro Arg Lys 
865                 870                 875                 880 


Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu Leu Gly 
                885                 890                 895     


Pro Ile Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Gln Val Ile 
            900                 905                 910         


Pro Leu Asn Asp Gly Lys Thr Val Ala Tyr Asn Ser Asn Ile Val Arg 
        915                 920                 925             


Val Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro Val Tyr 
    930                 935                 940                 


Thr Met Asp Ile Met Lys Lys Val Leu Pro Asn Lys Ala Ile Glu Pro 
945                 950                 955                 960 


Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Asp Tyr Thr Phe 
                965                 970                 975     


Arg Phe Ser Leu Tyr Pro Asn Asp Leu Ile Arg Ile Glu Leu Pro Arg 
            980                 985                 990         


Glu Lys Thr Val Lys Thr Thr Ala  Gly His Glu Ile Asn  Ile Lys Asp 
        995                 1000                 1005             


Val Phe  Val Tyr Tyr Lys Thr  Ile Asp Ser Ala Thr  Gly Gly Leu 
    1010                 1015                 1020             


Glu Leu  Ile Ser His Asp Asn  Ser Phe Ser Leu Arg  Gly Ile Gly 
    1025                 1030                 1035             


Ser Arg  Thr Leu Lys Arg Phe  Glu Lys Tyr Gln Val  Asp Val Leu 
    1040                 1045                 1050             


Gly Asn  Ile Tyr Lys Val Arg  Gly Glu Lys Arg Val  Gly Leu Ala 
    1055                 1060                 1065             


Ser Ser  Ala His Pro Glu Thr  Arg Glu Thr Ile Arg  Pro Leu Gln 
    1070                 1075                 1080             


Ser Thr  Arg Asp 
    1085         


<210>  617
<211>  1087
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  617

Met Lys Lys Leu Val Leu Gly Leu Asp Ile Gly Val Ala Ser Val Gly 
1               5                   10                  15      


Trp Gly Ile Ile Asp Ala Glu Asn Gly Asp Val Val Asp Thr Gly Val 
            20                  25                  30          


Arg Leu Phe Ser Ser Arg Asp Ala Ser Glu Asn Glu Ser Arg Arg Thr 
        35                  40                  45              


Ser Arg Gln Ser Arg Arg Leu Ser Arg Arg Lys Lys His Arg Arg Glu 
    50                  55                  60                  


Arg Ile Lys Gln Leu Leu Leu Ala His Asp Met Asp Ile Cys Asp Ile 
65                  70                  75                  80  


Val Asn Glu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Asn Glu Lys 
                85                  90                  95      


Leu Glu Val Ser Glu Ile Tyr Val Ala Leu Thr His Leu Ala Lys Arg 
            100                 105                 110         


Arg Gly Val Ser Tyr Leu Asp Asp Val Val Glu Glu Gly Gly Thr Ile 
        115                 120                 125             


Lys Ser Glu His Leu Asn Lys Asn Met Glu Glu Ile Lys Ser Gly Lys 
    130                 135                 140                 


Met Pro Cys Glu Ile Gln Leu Glu Arg Phe Glu Lys Tyr Gly Gln Val 
145                 150                 155                 160 


Arg Gly Met Ile Glu Ile Asn Gln Asp Thr Glu Glu Ser Ser Phe Leu 
                165                 170                 175     


Val Asn Ile Phe Arg Thr Ser Asp Tyr Ile Lys Glu Ala Lys Thr Ile 
            180                 185                 190         


Leu Ala Thr Gln Gln Lys Phe His Lys Gln Ile Thr Asn Glu Phe Ile 
        195                 200                 205             


Asp Glu Tyr Ile His Ile Met Ala Ser Lys Arg Asp Tyr Phe Ile Gly 
    210                 215                 220                 


Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Val Tyr Arg Thr Asn 
225                 230                 235                 240 


Gly Asp Thr Trp Asp Asn Leu Phe Glu Ile Leu Ile Gly Lys Cys Ser 
                245                 250                 255     


Val Phe Pro Glu Glu Thr Arg Ala Ala Arg Ser Ser Tyr Val Ala Gln 
            260                 265                 270         


Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Lys Ile Ser Asn Leu 
        275                 280                 285             


Glu Ser Gly Lys Leu Ser Ala Ser Gln Lys His Asp Ile Val Glu Lys 
    290                 295                 300                 


Val Lys Asn Ser Lys Lys Phe Gly Thr Lys Asp Leu Leu Lys Tyr Ile 
305                 310                 315                 320 


Ala Ser Val Cys Glu Cys Lys Ile Glu Asp Ile Gly Gly Cys Arg Val 
                325                 330                 335     


Asp Lys Asn Arg Lys Pro Ile Met His Thr Phe Glu Asn Tyr Arg Lys 
            340                 345                 350         


Val His Asn Ala Leu Asn Glu Ala Gly Val Asp Leu Arg Leu Ser Glu 
        355                 360                 365             


Thr Asp Tyr Asp Glu Leu Met Lys Ile Met Thr Leu Asn Ser Glu Thr 
    370                 375                 380                 


Leu Glu Ile Lys Lys Gln Cys Lys Glu Lys Leu Pro Gln Leu Thr Glu 
385                 390                 395                 400 


Asp Tyr Leu Asn Val Cys Leu Val Leu Asn Lys Lys Gly Ala Phe Ser 
                405                 410                 415     


Gly Trp His Ala Leu Ser Leu Lys Ala Met Lys Leu Met Met Pro Glu 
            420                 425                 430         


Leu Tyr Gly Thr Ser Lys Asn Gln Met Gln Ile Ile His Thr Met Gln 
        435                 440                 445             


Leu Gly Lys Ala Tyr Leu Lys Pro Leu Asn Gln Gln Lys Tyr Leu Asn 
    450                 455                 460                 


Ser Glu Ala Ile Leu Glu Glu Ile Tyr Asn Pro Ile Ala Ala Arg Ser 
465                 470                 475                 480 


Ile Arg Gln Thr Ile Lys Val Thr Asn Ala Ile Leu Lys Lys Tyr Gly 
                485                 490                 495     


His Leu Asp Ser Val Ile Ile Glu Met Pro Arg Asp Tyr Gln Thr Ser 
            500                 505                 510         


Glu Asp Glu Arg Lys Met Ile Glu Lys Met Gln Lys Glu Asn Glu Lys 
        515                 520                 525             


Glu Lys Lys Ala Ala Leu Lys Arg Ala Arg Glu Glu Tyr Pro Phe Pro 
    530                 535                 540                 


Asp Glu Ala Phe Arg Asn His Lys Glu Leu Ala Thr Lys Leu Arg Leu 
545                 550                 555                 560 


Trp Tyr Gln Gln Asp Gln Phe Cys Val Tyr Ser Gly Arg Lys Ile Ser 
                565                 570                 575     


Val Val Asp Leu Val Arg Asn Pro Asn Gln Phe Glu Ile Asp His Ile 
            580                 585                 590         


Ile Pro Gln Ser Val Ser Leu Asp Asp Ser Leu Asn Asn Lys Val Leu 
        595                 600                 605             


Cys Phe Ser Glu Glu Asn Gln Lys Lys Gly Gln Gln Thr Pro Phe Tyr 
    610                 615                 620                 


Tyr His Lys Gly Lys Ser Gly Asn Trp Thr Tyr Glu Gln Tyr Lys Leu 
625                 630                 635                 640 


Phe Val Glu Lys Leu Phe Asp Lys Lys Lys Ile Ser Asn Lys Lys Arg 
                645                 650                 655     


Glu Leu Leu Leu Phe Glu Glu Asp Ile Asn Lys Trp Asp Val Arg Arg 
            660                 665                 670         


Gly Phe Ile Ala Arg Asn Leu Val Asp Thr Arg Tyr Ala Ser Arg Val 
        675                 680                 685             


Ile Leu Asn Ser Tyr Gln Gln Tyr Phe Gly Asn His Glu Met Gly Thr 
    690                 695                 700                 


Lys Val Lys Val Val Arg Gly Lys Phe Thr Ser Gln Leu Arg Lys Lys 
705                 710                 715                 720 


Trp Arg Ile Phe Lys Asp Arg Asn Glu Ser His Ser His His Ala Ile 
                725                 730                 735     


Asp Ala Leu Ile Val Ala Ala Thr Ala Asn Leu Arg Leu Trp Lys Lys 
            740                 745                 750         


Asn Thr Leu Gly Val Ser Asp Glu Gly Lys Val Tyr Asp Lys Glu Thr 
        755                 760                 765             


Gly Glu Ile Leu Pro Phe Glu Lys Leu Thr Glu Ser Glu Tyr Asn Glu 
    770                 775                 780                 


Met Met Asn Lys Glu Pro Tyr Leu Asn Phe Lys Thr Lys Leu Gln Asn 
785                 790                 795                 800 


Ile Glu Thr Lys Val Lys Tyr Ser Tyr Arg Val Ser Lys Lys Val Asn 
                805                 810                 815     


Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ser Thr Arg Glu Arg Asp Gly 
            820                 825                 830         


Ile Asp Tyr Ile Val Gly Lys Ile Lys Asn Ile Tyr Asp Val Lys Thr 
        835                 840                 845             


Tyr Lys Asp Gln Phe Leu Lys Arg Tyr Lys Lys Asn Lys Glu Gln Phe 
    850                 855                 860                 


Leu Met Tyr Gln His Asp Pro Gln Thr Phe Ala Ile Leu Glu Asn Ile 
865                 870                 875                 880 


Met Ala Thr Tyr Gln Asp Ala Glu Asn Pro Phe Leu Ala Tyr Lys Lys 
                885                 890                 895     


Glu Tyr Gly Val Ile Arg Lys Tyr Ser Lys Lys Gly Gly Gly Ser Pro 
            900                 905                 910         


Val Thr Ser Leu Lys Phe Ile Asp Lys Val Leu Gly Gln Lys Ile Asp 
        915                 920                 925             


Leu Thr Ala Lys Asn Gln Ala Ala Leu Pro Lys Asn Lys Lys Val Val 
    930                 935                 940                 


Leu Gln Ser Ile Thr Pro Tyr Arg Ala Asp Val Tyr Tyr Asp Glu Ile 
945                 950                 955                 960 


Asp Lys Arg Tyr His Leu Ile Gly Val Lys Tyr Ser Leu Phe Ser Phe 
                965                 970                 975     


Ser Lys Gln Gly Tyr Val Ile Asn Pro Ile Ala Tyr Gln Lys Leu Leu 
            980                 985                 990         


Glu Lys Glu Gln Ile His Ser Glu  Met Lys Phe Cys Phe  Ser Leu Tyr 
        995                 1000                 1005             


Arg Glu  Asp Ile Ile Glu Phe  Gly Glu Asp Pro Asp  Ser Leu Thr 
    1010                 1015                 1020             


Arg Tyr  Arg Phe Trp Ser Lys  Asn Glu Ser Gly Lys  Asn Lys Ile 
    1025                 1030                 1035             


Glu Val  Lys Pro Ile Glu Lys  Pro Asn Phe Glu Lys  Gln Thr Val 
    1040                 1045                 1050             


Leu Thr  Ile Thr Lys Lys Ile  Lys Ile Leu Asn Lys  Tyr Gln Thr 
    1055                 1060                 1065             


Asp Val  Leu Gly Asn Ser Phe  Lys Ala Gln Glu Glu  Lys Asp Pro 
    1070                 1075                 1080             


Arg Asn  Ile Glu 
    1085         


<210>  618
<211>  1087
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  618

Met Arg Tyr Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Met Asn Leu Asp Ile Pro Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Lys 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Val Ile Arg Glu Gly Ile Leu 
65                  70                  75                  80  


Thr Lys Glu Glu Leu Asp Lys Leu Phe Glu Glu Lys His Glu Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ser Glu Arg Ser Asn Lys Glu Asn Ser Thr Met Leu 
    130                 135                 140                 


Lys His Ile Glu Glu Asn Arg Ala Ile Leu Ser Ser Tyr Arg Thr Val 
145                 150                 155                 160 


Gly Glu Met Ile Val Lys Asp Pro Lys Phe Ala Leu His Lys Arg Asn 
                165                 170                 175     


Lys Gly Glu Asn Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu Glu Arg 
            180                 185                 190         


Glu Ile Arg Leu Ile Phe Ser Lys Gln Arg Glu Phe Gly Asn Met Ser 
        195                 200                 205             


Cys Thr Glu Glu Phe Glu Asn Glu Tyr Ile Thr Ile Trp Ala Ser Gln 
    210                 215                 220                 


Arg Pro Val Ala Ser Lys Asp Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Ile Ala Trp Glu His Ile Asn Lys Leu Arg Leu Ile Ser 
            260                 265                 270         


Pro Ser Gly Ala Arg Gly Leu Thr Asp Glu Glu Arg Arg Leu Leu Tyr 
        275                 280                 285             


Glu Gln Ala Phe Gln Lys Asn Lys Ile Thr Tyr His Asp Ile Arg Thr 
    290                 295                 300                 


Leu Leu His Leu Pro Asp Asp Thr Tyr Phe Lys Gly Ile Val Tyr Asp 
305                 310                 315                 320 


Arg Gly Glu Ser Arg Lys Gln Asn Glu Asn Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ala Tyr His Gln Ile Arg Lys Ala Val Asp Lys Val Tyr Gly Lys 
            340                 345                 350         


Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Leu Phe Lys Asp Asp Ala Asp Ile His Ser Tyr Leu Arg 
    370                 375                 380                 


Asn Glu Tyr Glu Gln Asn Gly Lys Arg Met Pro Asn Leu Ala Asn Lys 
385                 390                 395                 400 


Val Tyr Asp Asn Glu Leu Ile Glu Glu Leu Leu Asn Leu Ser Phe Thr 
                405                 410                 415     


Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg Ser Ile Leu Pro Tyr 
            420                 425                 430         


Met Glu Gln Gly Glu Val Tyr Ser Ser Ala Cys Glu Arg Ala Gly Tyr 
        435                 440                 445             


Thr Phe Thr Gly Pro Lys Lys Lys Gln Lys Thr Met Leu Leu Pro Asn 
    450                 455                 460                 


Ile Pro Pro Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val Ser 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp Glu Arg 
            500                 505                 510         


Arg Lys Thr Lys Lys Glu Gln Asp Glu Asn Arg Lys Lys Asn Glu Thr 
        515                 520                 525             


Ala Ile Arg Gln Leu Met Glu Tyr Gly Leu Thr Leu Asn Pro Thr Gly 
    530                 535                 540                 


His Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly Arg Cys 
545                 550                 555                 560 


Ala Tyr Ser Leu Gln Pro Ile Glu Ile Glu Arg Leu Leu Glu Pro Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Arg Glu Asn Arg Glu Lys 
        595                 600                 605             


Gly Asn Arg Ile Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu Arg Trp 
    610                 615                 620                 


Gln Gln Phe Glu Thr Phe Val Leu Thr Asn Lys Gln Phe Ser Lys Lys 
625                 630                 635                 640 


Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu Glu Thr 
                645                 650                 655     


Glu Phe Lys Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Arg Phe 
            660                 665                 670         


Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Glu Ser Asp Asp 
        675                 680                 685             


Lys Gln Lys Val Tyr Thr Val Asn Gly Arg Val Thr Ala His Leu Arg 
    690                 695                 700                 


Ser Arg Trp Glu Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His His 
705                 710                 715                 720 


Ala Val Asp Ala Ala Ile Val Ala Cys Thr Thr Pro Ser Asp Ile Ala 
                725                 730                 735     


Lys Val Thr Ala Phe Tyr Gln Arg Arg Glu Gln Asn Lys Glu Leu Ala 
            740                 745                 750         


Lys Lys Thr Glu Pro His Phe Pro Gln Pro Trp Pro His Phe Ala Asp 
        755                 760                 765             


Glu Leu Arg Ala Arg Leu Ser Lys His Pro Lys Glu Ser Ile Lys Ala 
    770                 775                 780                 


Leu Asn Leu Gly Asn Tyr Asp Asp Gln Lys Leu Glu Ser Leu Gln Pro 
785                 790                 795                 800 


Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala His 
                805                 810                 815     


Gln Glu Thr Leu Arg Arg Tyr Val Gly Ile Asp Glu Arg Ser Gly Lys 
            820                 825                 830         


Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu Asp Ala 
        835                 840                 845             


Ser Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Arg Thr Tyr 
    850                 855                 860                 


Glu Ala Ile Arg Gln Arg Leu Leu Glu His Asn Asn Asp Pro Lys Lys 
865                 870                 875                 880 


Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu Pro Gly 
                885                 890                 895     


Pro Val Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Gln Val Ile 
            900                 905                 910         


Pro Leu Asn Asp Gly Lys Thr Val Ala Tyr Asn Ser Asn Ile Val Arg 
        915                 920                 925             


Val Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro Val Tyr 
    930                 935                 940                 


Thr Met Asp Ile Met Lys Gly Ile Leu Pro Asn Lys Ala Ile Glu Pro 
945                 950                 955                 960 


Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Asp Tyr Thr Phe 
                965                 970                 975     


Arg Phe Ser Leu Tyr Pro Asn Asp Leu Ile Arg Ile Glu Leu Pro Arg 
            980                 985                 990         


Glu Lys Thr Val Lys Thr Ala Ala  Gly Glu Glu Ile Asn  Val Lys Asp 
        995                 1000                 1005             


Val Phe  Val Tyr Tyr Lys Thr  Ile Asp Ser Ala Asn  Gly Gly Leu 
    1010                 1015                 1020             


Glu Leu  Ile Ser His Asp His  Arg Phe Ser Leu Arg  Gly Val Gly 
    1025                 1030                 1035             


Ser Arg  Thr Leu Lys Arg Phe  Glu Lys Tyr Gln Val  Asp Val Leu 
    1040                 1045                 1050             


Gly Asn  Ile Tyr Lys Val Arg  Gly Glu Lys Arg Val  Gly Leu Ala 
    1055                 1060                 1065             


Ser Ser  Ala His Ser Lys Thr  Gly Glu Thr Val Arg  Pro Leu Gln 
    1070                 1075                 1080             


Ser Thr  Arg Asp 
    1085         


<210>  619
<211>  1087
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  619

Met Arg Tyr Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Met Asn Leu Asp Ile Pro Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Lys 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Val Ile Arg Glu Gly Ile Leu 
65                  70                  75                  80  


Thr Lys Glu Glu Leu Asp Lys Leu Phe Glu Glu Lys His Glu Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ser Glu Arg Ser Asn Lys Glu Asn Ser Thr Met Leu 
    130                 135                 140                 


Lys His Ile Glu Glu Asn Arg Ala Ile Leu Ser Ser Tyr Arg Thr Val 
145                 150                 155                 160 


Gly Glu Met Ile Val Lys Asp Pro Lys Phe Ala Leu His Lys Arg Asn 
                165                 170                 175     


Lys Gly Glu Asn Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu Glu Arg 
            180                 185                 190         


Glu Ile Arg Leu Ile Phe Ser Lys Gln Arg Glu Phe Gly Asn Met Ser 
        195                 200                 205             


Cys Thr Glu Glu Phe Glu Asn Glu Tyr Ile Thr Ile Trp Ala Ser Gln 
    210                 215                 220                 


Arg Pro Val Ala Ser Lys Asp Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Ile Ala Trp Glu His Ile Asn Lys Leu Arg Leu Ile Ser 
            260                 265                 270         


Pro Ser Gly Ala Arg Gly Leu Thr Asp Glu Glu Arg Arg Leu Leu Tyr 
        275                 280                 285             


Glu Gln Ala Phe Gln Lys Asn Lys Ile Thr Tyr His Asp Ile Arg Thr 
    290                 295                 300                 


Leu Leu His Leu Pro Asp Asp Thr Tyr Phe Lys Gly Ile Val Tyr Asp 
305                 310                 315                 320 


Arg Gly Glu Ser Arg Lys Gln Asn Glu Asn Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ala Tyr His Gln Ile Arg Lys Ala Val Asp Lys Val Tyr Gly Lys 
            340                 345                 350         


Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Leu Phe Lys Asp Asp Ala Asp Ile His Ser Tyr Leu Arg 
    370                 375                 380                 


Asn Glu Tyr Glu Gln Asn Gly Lys Arg Met Pro Asn Leu Ala Asn Lys 
385                 390                 395                 400 


Val Tyr Asp Asn Glu Leu Ile Glu Glu Leu Leu Asn Leu Ser Phe Thr 
                405                 410                 415     


Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg Ser Ile Leu Pro Tyr 
            420                 425                 430         


Met Glu Gln Gly Glu Val Tyr Ser Ser Ala Cys Glu Arg Ala Gly Tyr 
        435                 440                 445             


Thr Phe Thr Gly Pro Lys Lys Lys Gln Lys Thr Met Leu Leu Pro Asn 
    450                 455                 460                 


Ile Pro Pro Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val Ser 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp Glu Arg 
            500                 505                 510         


Arg Lys Thr Lys Lys Glu Gln Asp Glu Asn Arg Lys Lys Asn Glu Thr 
        515                 520                 525             


Ala Ile Arg Gln Leu Met Glu Tyr Gly Leu Thr Leu Asn Pro Thr Gly 
    530                 535                 540                 


His Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly Arg Cys 
545                 550                 555                 560 


Ala Tyr Ser Leu Gln Pro Ile Glu Ile Glu Arg Leu Leu Glu Pro Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Arg Glu Asn Arg Glu Lys 
        595                 600                 605             


Gly Asn Arg Ile Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu Arg Trp 
    610                 615                 620                 


Gln Gln Phe Glu Thr Phe Val Leu Thr Asn Lys Gln Phe Ser Lys Lys 
625                 630                 635                 640 


Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu Glu Thr 
                645                 650                 655     


Glu Phe Lys Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Arg Phe 
            660                 665                 670         


Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Glu Ser Asp Asp 
        675                 680                 685             


Lys Gln Lys Val Tyr Thr Val Asn Gly Arg Val Thr Ala His Leu Arg 
    690                 695                 700                 


Ser Arg Trp Glu Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His His 
705                 710                 715                 720 


Ala Val Asp Ala Val Ile Val Ala Cys Thr Thr Pro Ser Asp Ile Ala 
                725                 730                 735     


Lys Val Thr Ala Phe Tyr Gln Arg Arg Glu Gln Asn Lys Glu Leu Ala 
            740                 745                 750         


Lys Lys Thr Glu Pro His Phe Pro Gln Pro Trp Pro His Phe Ala Asp 
        755                 760                 765             


Glu Leu Arg Ala Arg Leu Ser Lys His Pro Lys Glu Ser Ile Lys Ala 
    770                 775                 780                 


Leu Asn Leu Gly Asn Tyr Asp Asp Gln Lys Leu Glu Ser Leu Gln Pro 
785                 790                 795                 800 


Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala His 
                805                 810                 815     


Gln Glu Thr Leu Arg Arg Tyr Val Gly Ile Asp Glu Arg Ser Gly Lys 
            820                 825                 830         


Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu Asp Ala 
        835                 840                 845             


Ser Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Arg Thr Tyr 
    850                 855                 860                 


Glu Ala Ile Arg Gln Arg Leu Leu Glu His Asn Asn Asp Pro Lys Lys 
865                 870                 875                 880 


Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu Pro Gly 
                885                 890                 895     


Pro Val Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Gln Val Ile 
            900                 905                 910         


Pro Leu Asn Asp Gly Lys Thr Val Ala Tyr Asn Ser Asn Ile Val Arg 
        915                 920                 925             


Val Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro Val Tyr 
    930                 935                 940                 


Thr Met Asp Ile Met Lys Gly Ile Leu Pro Asn Lys Ala Ile Glu Pro 
945                 950                 955                 960 


Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Asp Tyr Thr Phe 
                965                 970                 975     


Arg Phe Ser Leu Tyr Pro Asn Asp Leu Ile Arg Ile Glu Leu Pro Arg 
            980                 985                 990         


Glu Lys Thr Val Lys Thr Ala Ala  Gly Glu Glu Ile Asn  Val Lys Asp 
        995                 1000                 1005             


Val Phe  Val Tyr Tyr Lys Thr  Ile Asp Ser Ala Asn  Gly Gly Leu 
    1010                 1015                 1020             


Glu Leu  Ile Ser His Asp His  Arg Phe Ser Leu Arg  Gly Val Gly 
    1025                 1030                 1035             


Ser Arg  Thr Leu Lys Arg Phe  Glu Lys Tyr Gln Val  Asp Val Leu 
    1040                 1045                 1050             


Gly Asn  Ile Tyr Lys Val Arg  Gly Glu Lys Arg Val  Gly Leu Ala 
    1055                 1060                 1065             


Ser Ser  Ala His Ser Lys Pro  Gly Lys Thr Ile Arg  Pro Leu Gln 
    1070                 1075                 1080             


Ser Thr  Arg Asp 
    1085         


<210>  620
<211>  1087
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  620

Met Arg Tyr Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Leu Asp Ile Pro Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Lys 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Ile Ile Arg Glu Gly Ile Leu 
65                  70                  75                  80  


Thr Lys Glu Glu Leu Asp Lys Leu Phe Glu Glu Lys His Glu Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ser Glu Arg Ser Asn Lys Glu Asn Ser Thr Met Leu 
    130                 135                 140                 


Lys His Ile Glu Glu Asn Arg Ala Ile Leu Ser Gly Tyr Arg Thr Val 
145                 150                 155                 160 


Gly Glu Met Ile Val Lys Asp Pro Lys Phe Ala Leu His Lys Arg Asn 
                165                 170                 175     


Lys Gly Glu Asn Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu Glu His 
            180                 185                 190         


Glu Ile Arg Leu Ile Phe Ser Lys Gln Arg Glu Phe Gly Asn Met Ser 
        195                 200                 205             


Cys Thr Glu Lys Phe Glu Asn Glu Tyr Ile Thr Ile Trp Ala Ser Gln 
    210                 215                 220                 


Arg Pro Val Ala Ser Lys Asp Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Ile Ala Trp Glu His Ile Asn Lys Leu Arg Leu Ile Phe 
            260                 265                 270         


Pro Ser Gly Ala Arg Gly Leu Thr Asp Glu Glu Arg Arg Leu Leu Tyr 
        275                 280                 285             


Glu Gln Ala Phe Gln Lys Asn Lys Ile Thr Tyr His Asp Ile Arg Thr 
    290                 295                 300                 


Leu Leu His Leu Pro Asp Asp Thr Tyr Phe Lys Gly Ile Val Tyr Asp 
305                 310                 315                 320 


Arg Gly Glu Ser Arg Lys Gln Asn Glu Asn Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ala Tyr His Gln Ile Arg Lys Ala Val Asp Lys Val Tyr Gly Lys 
            340                 345                 350         


Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Leu Phe Lys Asp Asp Ala Asp Ile His Ser Tyr Leu Arg 
    370                 375                 380                 


Asn Glu Tyr Glu Gln Asn Gly Lys Arg Met Pro Asn Leu Ala Asn Lys 
385                 390                 395                 400 


Val Tyr Asp Asn Glu Leu Ile Glu Glu Leu Leu Asn Leu Ser Phe Thr 
                405                 410                 415     


Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg Ser Ile Leu Pro Tyr 
            420                 425                 430         


Met Glu Gln Gly Glu Val Tyr Ser Ser Ala Cys Glu Arg Ala Gly Tyr 
        435                 440                 445             


Thr Phe Thr Gly Pro Lys Lys Lys Gln Lys Thr Met Leu Leu Pro Asn 
    450                 455                 460                 


Ile Pro Pro Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val Ser 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp Glu Arg 
            500                 505                 510         


Arg Lys Thr Lys Lys Glu Gln Asp Glu Asn Arg Lys Lys Asn Glu Thr 
        515                 520                 525             


Ala Ile Arg Gln Leu Met Glu Tyr Gly Leu Thr Leu Asn Pro Thr Gly 
    530                 535                 540                 


His Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly Arg Cys 
545                 550                 555                 560 


Ala Tyr Ser Leu Gln Pro Ile Glu Ile Glu Arg Leu Leu Glu Pro Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Arg Glu Asn Arg Glu Lys 
        595                 600                 605             


Gly Asn Arg Ile Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu Arg Trp 
    610                 615                 620                 


Gln Gln Phe Glu Thr Phe Val Leu Thr Asn Lys Gln Phe Ser Lys Lys 
625                 630                 635                 640 


Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu Glu Thr 
                645                 650                 655     


Glu Phe Lys Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Arg Phe 
            660                 665                 670         


Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Glu Ser Asp Asp 
        675                 680                 685             


Lys Gln Lys Val Tyr Thr Val Asn Gly Arg Val Thr Ser His Leu Arg 
    690                 695                 700                 


Ser Arg Trp Asp Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His His 
705                 710                 715                 720 


Ala Val Asp Ala Ala Ile Val Ala Cys Thr Thr Pro Ser Asp Ile Ala 
                725                 730                 735     


Lys Val Thr Ala Phe Tyr Gln Arg Arg Glu Gln Asn Lys Glu Leu Ala 
            740                 745                 750         


Lys Lys Thr Glu Pro His Phe Pro Gln Pro Trp Pro His Phe Ala Asp 
        755                 760                 765             


Glu Leu Arg Ala Arg Leu Ser Lys His Pro Lys Glu Ser Ile Lys Ala 
    770                 775                 780                 


Leu Asn Leu Gly Asn Tyr Asp Asp Gln Lys Leu Glu Ser Leu Gln Pro 
785                 790                 795                 800 


Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala His 
                805                 810                 815     


Gln Glu Thr Leu Arg Arg Tyr Ile Gly Ile Asp Glu Arg Ser Gly Lys 
            820                 825                 830         


Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu Asp Ala 
        835                 840                 845             


Ser Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Arg Thr Tyr 
    850                 855                 860                 


Glu Ala Ile Arg Gln Arg Leu Leu Glu His Asn Asn Asp Pro Lys Lys 
865                 870                 875                 880 


Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu Pro Gly 
                885                 890                 895     


Pro Ile Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Gln Val Ile 
            900                 905                 910         


Pro Leu Asn Asp Gly Lys Thr Val Ala Tyr Asn Ser Asn Ile Val Arg 
        915                 920                 925             


Val Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro Val Tyr 
    930                 935                 940                 


Thr Met Asp Ile Met Lys Gly Ile Leu Pro Asn Lys Ala Ile Glu Pro 
945                 950                 955                 960 


Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Asp Tyr Thr Phe 
                965                 970                 975     


Arg Phe Ser Leu Tyr Pro Asn Asp Leu Ile Arg Ile Glu Leu Pro Arg 
            980                 985                 990         


Glu Lys Thr Val Lys Thr Thr Thr  Gly Glu Glu Ile Asn  Val Lys Asp 
        995                 1000                 1005             


Val Phe  Val Tyr Tyr Lys Thr  Ile Asp Ser Ala Thr  Gly Gly Leu 
    1010                 1015                 1020             


Glu Leu  Ile Ser His Asp Asn  Arg Phe Ser Leu Arg  Gly Val Gly 
    1025                 1030                 1035             


Ser Arg  Thr Leu Lys Arg Phe  Glu Lys Tyr Gln Val  Asp Val Leu 
    1040                 1045                 1050             


Gly Asn  Ile Tyr Lys Val Arg  Gly Glu Lys Arg Val  Gly Leu Ala 
    1055                 1060                 1065             


Ser Ser  Ala His Ser Lys Thr  Gly Glu Thr Ile Arg  Pro Leu Gln 
    1070                 1075                 1080             


Ser Thr  Arg Asp 
    1085         


<210>  621
<211>  1087
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  621

Met Arg Tyr Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Leu Asp Ile Pro Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Lys 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Leu Ile Arg Glu Gly Val Leu 
65                  70                  75                  80  


Thr Lys Glu Glu Leu Asp Lys Leu Phe Glu Glu Lys His Glu Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Val Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Gln Ser Glu Arg Ser Asn Lys Glu Asn Ser Thr Met Leu 
    130                 135                 140                 


Lys His Ile Glu Glu Asn Arg Ala Ile Leu Ser Gly Tyr Arg Thr Val 
145                 150                 155                 160 


Gly Glu Met Ile Val Lys Asp Pro Lys Phe Ala Leu His Lys Arg Asn 
                165                 170                 175     


Lys Gly Glu Asn Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu Glu Arg 
            180                 185                 190         


Glu Ile Arg Leu Ile Phe Ser Lys Gln Arg Glu Phe Gly Asn Met Ser 
        195                 200                 205             


Cys Thr Glu Glu Phe Glu Asn Glu Tyr Ile Ala Ile Trp Ala Ser Gln 
    210                 215                 220                 


Arg Pro Val Ala Ser Lys Asp Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Ile Ala Trp Glu His Ile Asn Lys Leu Arg Leu Ile Ser 
            260                 265                 270         


Pro Ser Gly Thr Arg Gly Leu Thr Asp Glu Glu Arg Arg Leu Leu Tyr 
        275                 280                 285             


Glu Gln Ala Phe Gln Lys Asn Lys Ile Thr Tyr His Asp Ile Arg Thr 
    290                 295                 300                 


Leu Leu His Leu Pro Asp Asp Thr Tyr Phe Lys Gly Ile Val Tyr Asp 
305                 310                 315                 320 


Trp Gly Glu Ser Leu Lys Gln Asn Glu Asn Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ala Tyr His Gln Ile Arg Lys Ala Ile Asp Lys Val Tyr Gly Lys 
            340                 345                 350         


Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Leu Phe Lys Asp Asp Ala Asp Ile Arg Ser Tyr Leu Arg 
    370                 375                 380                 


Asn Glu Tyr Glu Gln Asn Gly Lys Arg Ile Ala Asn Leu Ala Asn Lys 
385                 390                 395                 400 


Val Tyr Asp Asn Glu Leu Ile Glu Glu Leu Leu Asn Leu Ser Phe Thr 
                405                 410                 415     


Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg Asn Ile Leu Pro Tyr 
            420                 425                 430         


Met Glu Gln Gly Glu Val Tyr Ser Ser Ala Cys Glu Lys Ala Gly Tyr 
        435                 440                 445             


Thr Phe Thr Gly Pro Lys Lys Lys Gln Lys Thr Val Leu Leu Pro Thr 
    450                 455                 460                 


Ile Pro Pro Ile Val Asn Pro Val Val Met Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val Ser 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp Glu Arg 
            500                 505                 510         


Arg Lys Thr Lys Arg Glu Gln Asp Glu Asn Arg Lys Arg Asn Glu Thr 
        515                 520                 525             


Ala Ile Arg Gln Leu Met Glu Tyr Gly Leu Thr Leu Asn Pro Thr Gly 
    530                 535                 540                 


Leu Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly Arg Cys 
545                 550                 555                 560 


Ala Tyr Ser Leu Gln Pro Ile Glu Ile Glu Arg Leu Leu Glu Pro Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Lys Glu Asn Arg Glu Lys 
        595                 600                 605             


Gly Asn Arg Ile Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu Arg Trp 
    610                 615                 620                 


Gln Gln Phe Glu Thr Phe Val Leu Thr Asn Lys Gln Phe Ser Lys Lys 
625                 630                 635                 640 


Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu Glu Met 
                645                 650                 655     


Glu Phe Lys Ser Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Arg Phe 
            660                 665                 670         


Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Glu Ser Asp Asp 
        675                 680                 685             


Lys Gln Lys Val Tyr Thr Val Asn Gly Arg Val Thr Ser His Leu Arg 
    690                 695                 700                 


Ser Arg Trp Asp Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His His 
705                 710                 715                 720 


Ala Val Asp Ala Ala Ile Val Ala Cys Thr Thr Pro Ser Asp Ile Ala 
                725                 730                 735     


Lys Val Thr Ala Phe Tyr Gln Arg Arg Glu Gln Asn Lys Glu Leu Ala 
            740                 745                 750         


Lys Lys Thr Glu Pro Gln Phe Pro Gln Pro Trp Pro His Phe Ala Asp 
        755                 760                 765             


Glu Leu Arg Ala Arg Leu Ser Lys His Pro Lys Glu Ser Ile Lys Ala 
    770                 775                 780                 


Leu Asn Leu Gly Asn Tyr Asp Asp Gln Lys Leu Glu Ser Leu Gln Pro 
785                 790                 795                 800 


Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala His 
                805                 810                 815     


Gln Glu Thr Leu Arg Arg Tyr Ile Gly Ile Asp Glu Arg Ser Gly Lys 
            820                 825                 830         


Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu Asp Ala 
        835                 840                 845             


Ser Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Arg Thr Tyr 
    850                 855                 860                 


Glu Ala Ile Arg Gln Arg Leu Leu Glu His Asn Asn Asp Pro Lys Lys 
865                 870                 875                 880 


Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu Pro Gly 
                885                 890                 895     


Pro Ile Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Gln Val Ile 
            900                 905                 910         


Pro Leu Asn Asp Gly Lys Thr Val Ala Tyr Asn Ser Asn Ile Val Arg 
        915                 920                 925             


Ile Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro Val Tyr 
    930                 935                 940                 


Thr Met Asp Ile Met Lys Gly Val Leu Pro Asn Lys Ala Ile Glu Pro 
945                 950                 955                 960 


Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Asp Tyr Thr Phe 
                965                 970                 975     


Arg Phe Ser Leu Tyr Pro Asn Asp Leu Ile Arg Ile Glu Leu Pro Arg 
            980                 985                 990         


Glu Lys Thr Val Lys Thr Ala Ala  Gly Glu Glu Ile Asn  Ile Lys Asp 
        995                 1000                 1005             


Met Phe  Val Tyr Tyr Lys Thr  Ile Asp Ser Ala Asn  Gly Gly Leu 
    1010                 1015                 1020             


Glu Leu  Ile Ser His Asp Asn  Ser Phe Ser Leu Arg  Gly Val Gly 
    1025                 1030                 1035             


Ser Arg  Thr Leu Lys Arg Phe  Glu Lys Tyr Gln Val  Asp Val Leu 
    1040                 1045                 1050             


Gly Asn  Ile Tyr Lys Val Arg  Gly Glu Lys Arg Val  Gly Leu Ala 
    1055                 1060                 1065             


Ser Ser  Ala His Ser Lys Thr  Gly Glu Thr Ile Arg  Pro Leu Gln 
    1070                 1075                 1080             


Ser Thr  Arg Asp 
    1085         


<210>  622
<211>  1088
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  622

Met Pro Lys Glu Phe Asp Pro Leu Lys Pro Ala Lys Tyr Arg Leu Ala 
1               5                   10                  15      


Leu Asp Ile Gly Ser Lys Ser Ile Gly Phe Ala Ile Ile Phe Leu Lys 
            20                  25                  30          


Glu Asp Val Asn Lys Leu Leu Lys Pro Tyr Ala Ile Val Arg Ser Gly 
        35                  40                  45              


Val Arg Ile Phe Gly Asp Gly Arg Thr Pro Ala Ala Pro Gly Gln Leu 
    50                  55                  60                  


Gly Ser Ser Leu Ala Ala Glu Arg Arg Val Lys Arg Ser Ile Arg Arg 
65                  70                  75                  80  


Gln Tyr Ala Arg Lys Arg Lys Arg Leu Gln Arg Leu Leu Arg Asn Leu 
                85                  90                  95      


Lys Glu Leu Gly Phe Phe Pro Asp Asp Ser Lys Leu Cys Lys Ser Phe 
            100                 105                 110         


Glu Asp Leu Asn Pro Tyr Glu Leu Arg His Lys Gly Leu Thr Lys Glu 
        115                 120                 125             


Leu Lys Pro His Glu Phe Gly Arg Leu Leu Met His Met Ala Lys Asn 
    130                 135                 140                 


Arg Gly Phe Gln Ser Asn Arg Lys Val Asp Leu Leu Ala Asp Asn Ile 
145                 150                 155                 160 


Asp Ser Gln Ala Lys Ala Glu Thr Ser Ala Met Lys Ser Ala Ile Ala 
                165                 170                 175     


Thr Leu Gln Ser Arg Leu Asn Gly Glu Phe Glu Thr Leu Gly Ala Tyr 
            180                 185                 190         


Leu Tyr Glu Arg Phe Lys Lys Gly Glu Thr Ile Lys Ser Ser Lys Thr 
        195                 200                 205             


Gln Lys Gly Gln Lys Ala Glu Tyr Glu Phe Ser Ile Ser Arg Arg Met 
    210                 215                 220                 


Val Lys Gln Glu Phe Asp Arg Leu Trp Ala Ala Gln Glu Gln Phe Asn 
225                 230                 235                 240 


Pro Glu Leu Phe Asn Glu Gln Ala Lys Asn Thr Leu Lys Asp Thr Ile 
                245                 250                 255     


Phe Phe Gln Arg Asn Leu Leu Pro Gln Lys Val Gly Lys Cys Thr Phe 
            260                 265                 270         


Leu Pro Glu His Glu Arg Ala Ser Lys Ala Leu Pro Ser Ser Gln Tyr 
        275                 280                 285             


Phe Arg Ile Ile Ser Glu Leu Asn Lys Ile Ser Phe Thr Asp Ala Glu 
    290                 295                 300                 


Gly Leu Ile Leu Pro Ile Ser Leu Glu Gln Arg Asn Lys Leu Leu Lys 
305                 310                 315                 320 


Ser Leu Arg Ile Lys Gln Lys Leu Ser Phe Asp Arg Ile Arg Thr Glu 
                325                 330                 335     


Leu Asn Leu Asp Ser Thr Val Leu Ile Asn Leu Glu His Ala Ser Lys 
            340                 345                 350         


Asp Ser Ile Glu Gly Asp Lys Thr Gly Tyr Leu Leu Ser Lys Lys Glu 
        355                 360                 365             


Tyr Phe Gly Pro Ala Trp Arg Asp Leu Ser Leu Glu Thr Gln Asp Ala 
    370                 375                 380                 


Ile Val Thr Ala Leu Leu Thr His Glu Asn Glu Ala Glu Leu Val Gln 
385                 390                 395                 400 


Lys Leu Val Asp Asp Phe Asn Ile Ala His Asp Val Ala Lys Ala Leu 
                405                 410                 415     


Ala Asn Ala Arg Leu Glu Ser Gln Tyr Met Arg Leu Ser Lys Glu Ala 
            420                 425                 430         


Ile Glu Arg Leu Ile Pro Glu Leu Glu Lys Gly Val Asp Ser Gln Asp 
        435                 440                 445             


Gly Tyr Lys Val Pro Val Lys Tyr His Glu Ala Thr Glu Ala Ala Gly 
    450                 455                 460                 


Phe Gly His His Ser Ala Leu Gln Glu Asn Trp Ala Asp Phe Pro Thr 
465                 470                 475                 480 


Leu Asp Leu Lys Gln Leu His Ser Asp Asp Asn Asp Lys Tyr Thr Val 
                485                 490                 495     


Ala Glu Leu Pro Leu Pro Tyr Tyr Gly Lys Val Ile Thr Arg Gln Val 
            500                 505                 510         


Ala Phe Gly Thr Gly Asp Val Asn Asp Pro Val Glu Lys Arg Phe Gly 
        515                 520                 525             


Lys Ile Ala Asn Pro Thr Val His Ile Cys Leu Asn Gln Val Arg Val 
    530                 535                 540                 


Leu Leu Asp Glu Ile Ile Ala Lys Tyr Gly Tyr Pro Tyr Glu Val Asn 
545                 550                 555                 560 


Ile Glu Leu Ala Arg Glu Leu Lys Gln Asn His Glu Gln Arg Gln Arg 
                565                 570                 575     


Ser Ile Lys Gln Gln Ala Ala Asn Lys Lys Glu Asn Glu Glu Ile Arg 
            580                 585                 590         


Arg Glu Ile Lys Gln Ala Tyr Lys Val Glu Lys Ala Arg Ile Ile Thr 
        595                 600                 605             


Asp Ser Glu Ile Ser Arg Ala Asp Ile Glu Lys Trp Arg Leu Trp Arg 
    610                 615                 620                 


Arg Ser Lys Ser Lys Leu Cys Val Tyr Ser Gly Lys Lys Ile Cys Glu 
625                 630                 635                 640 


Thr Met Ile Leu Asn Asn Glu Val Glu Val Asp His Ile Leu Pro Arg 
                645                 650                 655     


Ser Arg Thr Gly Asp Asn Ser Tyr Gln Asn Lys Val Leu Val Leu Asn 
            660                 665                 670         


Lys Ala Asn Arg Ile Lys Ser Asn Arg Thr Pro Trp Glu Ala Lys Glu 
        675                 680                 685             


Leu Phe Glu Ala Gln Gly Trp Ser Tyr Glu Asp Ile Leu Asn Arg Met 
    690                 695                 700                 


Arg Ala Leu Asp Lys His Gln Leu Phe Arg Phe Ala Glu Asn Ser Met 
705                 710                 715                 720 


Asp Ile Trp Asp Lys Asn Tyr Arg Ser Phe Glu Ala Arg Ala Leu Asn 
                725                 730                 735     


Asp Thr Arg Tyr Ile Ser Arg Val Val Lys Asp Tyr Leu Lys Val Val 
            740                 745                 750         


Cys His Lys Asp Arg Ile Val Val Ser Asn Gly Ala His Thr Ala Leu 
        755                 760                 765             


Ile Arg Arg Glu Phe Gly Leu Asn Asp Leu Leu Gly Lys Lys Gly Glu 
    770                 775                 780                 


Lys Asn Arg Asp Asp His Arg His His Ala Ile Asp Ala Cys Val Ile 
785                 790                 795                 800 


Gly Ala Leu Asp Arg Ser Met Leu Arg Lys Leu Gln Ile Ala Met Glu 
                805                 810                 815     


Val Ala Glu Lys Gln Asn Ser Ala Arg Asn Phe Val Gly Leu Gln Glu 
            820                 825                 830         


Pro Trp Phe Asn Phe Ser Thr Gln Val Arg Arg Ser Ile Gly Gln Ile 
        835                 840                 845             


Tyr Val Ser His Lys Pro Asn His Asn Tyr Glu Gly Glu Ile Phe Glu 
    850                 855                 860                 


Ala Ser Ile Tyr Gly Leu Arg Leu Ile Ser Asp Lys Arg Thr Glu Lys 
865                 870                 875                 880 


Tyr Val Gly Ile Lys Arg Asn Pro Glu Thr Gly Lys Thr Asp Lys Pro 
                885                 890                 895     


Lys Lys Ser Val Val Val Ile Ser Asp Pro Lys Thr Gln Asn Arg Tyr 
            900                 905                 910         


Gly Leu Arg His Lys Phe His Ser Glu Gly Leu Pro Lys Lys Tyr Tyr 
        915                 920                 925             


Gly Leu Ser Ser Ser Ser Val Phe Cys Leu Glu Ile Tyr Arg Asp Glu 
    930                 935                 940                 


Lys Gly Asn Trp Gln Ala Tyr Thr Ile Pro Arg Tyr Glu Ala Tyr Arg 
945                 950                 955                 960 


Ile Val Lys Arg Arg Gly Val Asn Ala Leu Tyr Asp Asn Lys Arg Ser 
                965                 970                 975     


Asn Phe Glu Gly Arg Pro Leu Val Met Arg Leu Met Arg Asp Asp Cys 
            980                 985                 990         


Ile Ala Phe Glu Lys Asn Gly Val  Lys Glu Val Tyr Arg  Ile Gln Lys 
        995                 1000                 1005             


Ile Asn  Gly Leu Lys Lys Asn  Ile Thr Leu Thr Arg  His Phe Asp 
    1010                 1015                 1020             


Ala Asn  Val Asp Asp Arg Tyr  Arg Arg Met Leu Lys  Ala Gln Lys 
    1025                 1030                 1035             


Ala Glu  Lys Ala Gly Glu Asp  Phe Asp Ser Glu Asp  Leu Cys Asp 
    1040                 1045                 1050             


Asp Leu  Val Phe Ser Ser Phe  Gly Ala Ser Ser Leu  Tyr Ser Asn 
    1055                 1060                 1065             


Lys Ala  Arg Lys Val Thr Ile  Ser Pro Met Gly Val  Leu Arg Asp 
    1070                 1075                 1080             


Pro Gly  Phe Lys Gly 
    1085             


<210>  623
<211>  1088
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  623

Met Lys Lys Leu Val Leu Gly Leu Asp Ile Gly Val Ala Ser Val Gly 
1               5                   10                  15      


Trp Gly Ile Ile Asp Ala Glu Asn Gly Asp Val Leu Asp Ala Gly Val 
            20                  25                  30          


Arg Leu Phe Ser Ser Arg Asp Ala Ser Glu Asn Glu Ser Arg Arg Thr 
        35                  40                  45              


Ser Arg Arg Gln Ser Arg Arg Leu Ser Arg Arg Lys Lys His Arg Arg 
    50                  55                  60                  


Glu Arg Ile Lys Gln Leu Leu Leu Ala His Asp Met Asp Val Cys Asp 
65                  70                  75                  80  


Ile Val Asn Glu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu Asn Glu 
                85                  90                  95      


Lys Leu Glu Ile Ser Glu Ile Tyr Val Ala Leu Thr His Leu Ala Lys 
            100                 105                 110         


Arg Arg Gly Val Ser Tyr Leu Asp Asp Val Val Glu Glu Gly Gly Ala 
        115                 120                 125             


Ile Lys Ser Glu His Leu Asn Lys Asn Met Glu Glu Ile Lys Ser Gly 
    130                 135                 140                 


Lys Met Pro Cys Glu Ile Gln Leu Glu Arg Phe Glu Lys Tyr Gly Gln 
145                 150                 155                 160 


Val Arg Gly Met Ile Glu Ile Asn Gln Asp Thr Glu Glu Ser Ser Phe 
                165                 170                 175     


Leu Val Asn Ile Phe Arg Thr Ser Asp Tyr Ile Lys Glu Ala Lys Thr 
            180                 185                 190         


Ile Leu Ala Thr Gln Gln Lys Phe His Lys Gln Ile Thr Asn Glu Phe 
        195                 200                 205             


Ile Asp Glu Tyr Ile Asn Ile Met Ala Ser Lys Arg Asp Tyr Phe Ile 
    210                 215                 220                 


Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Val Tyr Arg Thr 
225                 230                 235                 240 


Asn Gly Asp Thr Trp Asp Asn Leu Phe Glu Ile Leu Ile Gly Lys Cys 
                245                 250                 255     


Ser Val Phe Pro Glu Glu Thr Arg Ala Ala Arg Ser Ser Tyr Val Ala 
            260                 265                 270         


Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Lys Ile Ser Ser 
        275                 280                 285             


Leu Glu Ser Gly Lys Leu Ser Thr Ser Gln Lys His Asp Ile Val Glu 
    290                 295                 300                 


Lys Val Lys Asn Ser Lys Lys Phe Gly Thr Lys Asp Leu Leu Lys Tyr 
305                 310                 315                 320 


Ile Ala Ser Val Cys Glu Cys Lys Ile Glu Asn Ile Asp Gly Cys Arg 
                325                 330                 335     


Lys Asp Lys Asn Arg Lys Pro Ile Met His Thr Phe Glu Asn Tyr Arg 
            340                 345                 350         


Lys Val His Asn Ala Leu Asn Glu Ala Gly Val Asp Leu Arg Leu Ser 
        355                 360                 365             


Glu Ala Asp Tyr Asp Glu Leu Met Lys Ile Met Thr Leu Asn Ser Glu 
    370                 375                 380                 


Thr Leu Glu Ile Lys Lys Gln Cys Lys Glu Lys Leu Pro His Leu Thr 
385                 390                 395                 400 


Glu Asp Tyr Leu Asn Val Cys Leu Val Leu Asn Lys Lys Gly Ala Phe 
                405                 410                 415     


Ser Gly Trp His Ala Leu Ser Leu Lys Ala Met Lys Leu Met Met Pro 
            420                 425                 430         


Glu Leu Tyr Gly Thr Ser Lys Asn Gln Met Gln Ile Ile His Thr Met 
        435                 440                 445             


Gln Leu Gly Lys Ala Tyr Ile Lys Pro Leu Asn Gln Gln Lys Tyr Leu 
    450                 455                 460                 


Asn Ser Glu Ala Ile Leu Glu Glu Ile Tyr Asn Pro Ile Ala Ala Arg 
465                 470                 475                 480 


Ser Ile Arg Gln Thr Ile Lys Val Thr Asn Ala Ile Leu Lys Lys Tyr 
                485                 490                 495     


Gly Pro Leu Asp Ser Val Ile Ile Glu Met Pro Arg Asp Tyr Gln Thr 
            500                 505                 510         


Ser Glu Asp Glu Arg Lys Met Ile Glu Lys Met Gln Lys Glu Asn Glu 
        515                 520                 525             


Lys Glu Lys Lys Ala Ala Leu Lys Arg Ala Arg Glu Glu Tyr Pro Phe 
    530                 535                 540                 


Pro Asp Glu Ala Phe Arg Asn His Lys Glu Leu Ala Thr Lys Leu Arg 
545                 550                 555                 560 


Leu Trp Tyr Gln Gln Asp Gln Phe Cys Val Tyr Ser Gly Arg Lys Ile 
                565                 570                 575     


Ser Val Val Asp Leu Val Arg Asn Pro Asn Gln Phe Glu Ile Asp His 
            580                 585                 590         


Ile Ile Pro Gln Ser Val Ser Leu Asp Asp Ser Leu Asn Asn Lys Val 
        595                 600                 605             


Leu Cys Phe Ser Glu Glu Asn Gln Lys Lys Gly Gln Gln Thr Pro Phe 
    610                 615                 620                 


Tyr Tyr His Lys Gly Lys Ser Gly Asn Trp Thr Tyr Glu Gln Tyr Lys 
625                 630                 635                 640 


Leu Phe Val Glu Lys Leu Phe Asp Lys Lys Lys Ile Ser Asn Lys Lys 
                645                 650                 655     


Arg Glu Leu Leu Leu Phe Glu Glu Asp Ile Asn Lys Trp Asp Val Arg 
            660                 665                 670         


Arg Gly Phe Ile Ala Arg Asn Leu Val Asp Thr Arg Tyr Ala Ser Arg 
        675                 680                 685             


Val Ile Leu Asn Ser Tyr Gln Gln Tyr Phe Gly Asn His Glu Met Gly 
    690                 695                 700                 


Thr Lys Val Lys Val Val Arg Gly Lys Phe Thr Ser Gln Leu Arg Lys 
705                 710                 715                 720 


Lys Trp Lys Ile Phe Lys Asp Arg Asn Glu Ser His Ser His His Ala 
                725                 730                 735     


Ile Asp Ala Leu Ile Val Ala Ala Thr Ala Asn Leu Arg Leu Trp Lys 
            740                 745                 750         


Lys Asn Lys Leu Gly Ile Ser Asp Glu Gly Lys Val Tyr Asp Lys Glu 
        755                 760                 765             


Thr Gly Glu Ile Leu Pro Phe Glu Lys Leu Thr Ala Ser Glu Tyr Asn 
    770                 775                 780                 


Glu Met Met Asn Lys Glu Pro Tyr Leu Asn Phe Lys Thr Lys Leu Gln 
785                 790                 795                 800 


Asn Ile Glu Thr Lys Val Lys Tyr Ser Tyr Lys Val Ser Lys Lys Val 
                805                 810                 815     


Asn Arg Lys Ile Ser Asn Asp Thr Ile Tyr Ser Thr Arg Glu Arg Asp 
            820                 825                 830         


Gly Ile Asp Tyr Ile Val Gly Lys Ile Lys Asn Ile Tyr Asp Val Lys 
        835                 840                 845             


Thr Tyr Lys Asp Gln Phe Leu Lys Arg Tyr Lys Lys Asn Lys Glu Gln 
    850                 855                 860                 


Phe Leu Met Tyr Gln His Asp Pro Gln Thr Phe Ala Ile Leu Glu Ser 
865                 870                 875                 880 


Ile Met Ala Thr Tyr Pro Asp Ala Glu Asn Pro Phe Leu Ala Tyr Lys 
                885                 890                 895     


Lys Glu His Gly Val Ile Arg Lys Tyr Ala Lys Asn Lys Ala Gly Ala 
            900                 905                 910         


Pro Val Thr Ser Leu Lys Phe Ile Asp Gly Ala Leu Gly Gln Lys Ile 
        915                 920                 925             


Asp Val Thr Tyr Lys Asn Lys Ala Ala Leu Pro Lys Asn Lys Lys Val 
    930                 935                 940                 


Val Leu Gln Ser Ile Lys Pro Tyr Arg Ala Asp Val Tyr Tyr Asp Glu 
945                 950                 955                 960 


Gly Asn Lys Arg Tyr His Leu Ile Gly Val Lys Tyr Ser Leu Phe Ser 
                965                 970                 975     


Phe Ser Lys Gln Gly Tyr Val Ile Asn Pro Ile Ala Tyr Gln Lys Leu 
            980                 985                 990         


Leu Glu Lys Glu Gln Ile His Ser  Glu Met Lys Phe Cys  Phe Ser Leu 
        995                 1000                 1005             


Tyr Arg  Glu Asp Ile Ile Glu  Ser Gly Glu Asp Pro  Asp Ser Leu 
    1010                 1015                 1020             


Thr Arg  Tyr Arg Phe Trp Ser  Lys Asn Glu Ser Gly  Lys Asn Lys 
    1025                 1030                 1035             


Ile Glu  Val Lys Pro Ile Glu  Lys Pro Asn Phe Glu  Asp Arg Lys 
    1040                 1045                 1050             


Val Ile  Thr Ile Thr Lys Lys  Ile Lys Ile Leu Asn  Lys Tyr Gln 
    1055                 1060                 1065             


Thr Asp  Val Leu Gly Asn Ser  Phe Lys Ala Gln Glu  Glu Lys Asp 
    1070                 1075                 1080             


Pro Arg  Ser Ile Glu 
    1085             


<210>  624
<211>  1088
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  624

Met Asn Tyr Lys Met Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Leu Asp Leu Lys Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Lys Ala Glu His Pro Gln Asn Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Ile Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Lys 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Leu Val Ser Glu Asn Val Leu 
65                  70                  75                  80  


Thr Lys Glu Glu Met Asn Leu Leu Phe Lys Gln Lys Lys Gln Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Asp Ala Leu Glu Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ser Glu Arg Asn Ser Lys Glu Ser Ser Glu Phe Leu 
    130                 135                 140                 


Lys Asn Ile Glu Glu Asn Gln Ser Ile Leu Ala Gln Tyr Arg Ser Val 
145                 150                 155                 160 


Gly Glu Met Ile Val Lys Asp Ser Lys Phe Ala Tyr His Lys Arg Asn 
                165                 170                 175     


Lys Leu Asp Ser Tyr Ser Asn Met Ile Ala Arg Asp Asp Leu Glu Arg 
            180                 185                 190         


Glu Ile Lys Leu Ile Phe Glu Lys Gln Arg Glu Phe Asn Asn Pro Val 
        195                 200                 205             


Cys Thr Glu Arg Leu Glu Glu Lys Tyr Leu Asn Ile Trp Ser Ser Gln 
    210                 215                 220                 


Arg Pro Phe Ala Ser Lys Glu Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Ile Val Trp Glu His Ile Asn Lys Leu Arg Leu Val Ser 
            260                 265                 270         


Pro Asp Glu Thr Arg Ala Leu Thr Glu Ile Glu Arg Asn Leu Leu Tyr 
        275                 280                 285             


Lys Gln Ala Phe Ser Lys Asn Lys Met Thr Tyr Tyr Asp Ile Arg Lys 
    290                 295                 300                 


Leu Leu Asn Leu Ser Asp Asp Ile His Phe Lys Gly Leu Leu Tyr Asp 
305                 310                 315                 320 


Pro Lys Ser Ser Leu Lys Gln Ile Glu Asn Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ser Tyr His Lys Ile Arg Lys Cys Ile Glu Asn Val Tyr Gly Lys 
            340                 345                 350         


Asp Gly Ile Arg Met Phe Asn Glu Thr Asp Ile Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Ile Phe Lys Asp Asp Glu Asp Ile Val Ala Tyr Leu Gln 
    370                 375                 380                 


Asn Glu Tyr Ile Thr Lys Asn Gly Lys Arg Val Ser Asn Leu Ala Asn 
385                 390                 395                 400 


Lys Val Tyr Asp Lys Ser Leu Ile Asp Glu Leu Leu Asn Leu Ser Phe 
                405                 410                 415     


Ser Lys Phe Ala His Leu Ser Met Lys Ala Ile Arg Asn Ile Leu Pro 
            420                 425                 430         


Tyr Met Glu Gln Gly Glu Ile Tyr Ser Lys Ala Cys Glu Leu Ala Gly 
        435                 440                 445             


Tyr Asn Phe Thr Gly Pro Lys Lys Lys Glu Lys Ala Leu Leu Leu Pro 
    450                 455                 460                 


Val Ile Pro Asn Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr Gln 
465                 470                 475                 480 


Ser Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val 
                485                 490                 495     


Ser Ile His Ile Glu Leu Ala Arg Asp Leu Ser His Ser Phe Asp Glu 
            500                 505                 510         


Arg Lys Lys Ile Gln Lys Asp Gln Thr Glu Asn Arg Lys Lys Asn Glu 
        515                 520                 525             


Thr Ala Ile Lys Gln Leu Ile Glu Tyr Glu Leu Thr Lys Asn Pro Thr 
    530                 535                 540                 


Gly Leu Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Gln Gly Arg 
545                 550                 555                 560 


Cys Met Tyr Ser Leu Lys Pro Ile Glu Leu Glu Arg Leu Leu Glu Pro 
                565                 570                 575     


Gly Tyr Val Glu Val Asp His Ile Leu Pro Tyr Ser Arg Ser Leu Asp 
            580                 585                 590         


Asp Ser Tyr Ala Asn Lys Val Leu Val Leu Thr Lys Glu Asn Arg Glu 
        595                 600                 605             


Lys Gly Asn His Thr Pro Val Glu Tyr Leu Gly Leu Gly Ser Glu Arg 
    610                 615                 620                 


Trp Lys Lys Phe Glu Lys Phe Val Leu Ala Asn Lys Gln Phe Ser Lys 
625                 630                 635                 640 


Lys Lys Lys Gln Asn Leu Leu Arg Leu Arg Tyr Glu Glu Thr Glu Glu 
                645                 650                 655     


Lys Glu Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Lys 
            660                 665                 670         


Phe Phe Ala Asn Phe Ile Lys Glu His Leu Lys Phe Ala Asp Gly Asp 
        675                 680                 685             


Gly Gly Gln Lys Val Tyr Thr Ile Asn Gly Lys Ile Thr Ala His Leu 
    690                 695                 700                 


Arg Ser Arg Trp Asp Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His 
705                 710                 715                 720 


His Ala Val Asp Ala Val Ile Val Ala Cys Ala Thr Gln Gly Met Ile 
                725                 730                 735     


Lys Lys Ile Thr Glu Phe Tyr Lys Ala Arg Glu Gln Asn Lys Glu Ser 
            740                 745                 750         


Ala Lys Lys Lys Glu Pro Ile Phe Pro Gln Pro Trp Pro His Phe Ala 
        755                 760                 765             


Asp Glu Leu Lys Ala Arg Leu Ser Lys Phe Pro Gln Glu Ser Ile Glu 
    770                 775                 780                 


Ala Phe Ala Leu Gly Asn Tyr Asp Arg Lys Lys Leu Glu Ser Leu Arg 
785                 790                 795                 800 


Pro Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala 
                805                 810                 815     


His Gln Glu Thr Leu Arg Arg Cys Val Gly Ile Asp Glu Gln Ser Gly 
            820                 825                 830         


Lys Ile Gln Thr Ala Val Lys Thr Lys Leu Ser Asp Ile Lys Leu Asp 
        835                 840                 845             


Lys Asp Gly His Phe Pro Met Tyr Gln Lys Glu Ser Asp Pro Arg Thr 
    850                 855                 860                 


Tyr Glu Ala Ile Arg Gln Arg Leu Leu Glu His Asn Asn Asp Pro Lys 
865                 870                 875                 880 


Lys Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu Pro 
                885                 890                 895     


Gly Pro Val Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Lys Val 
            900                 905                 910         


Val His Leu Asp Gly Ser Lys Thr Val Ala Tyr Asn Ser Asn Ile Val 
        915                 920                 925             


Arg Thr Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro Val 
    930                 935                 940                 


Tyr Thr Met Asp Ile Met Lys Gly Thr Leu Pro Asn Lys Ala Ile Glu 
945                 950                 955                 960 


Ala Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Glu Tyr Thr 
                965                 970                 975     


Phe Gln Phe Ser Leu Phe Pro Asn Asp Leu Val Arg Ile Val Leu Pro 
            980                 985                 990         


Arg Glu Lys Thr Ile Lys Thr Ser  Thr Asn Glu Glu Ile  Ile Ile Lys 
        995                 1000                 1005             


Asp Ile  Phe Ala Tyr Tyr Lys  Thr Ile Asp Ser Ala  Thr Gly Gly 
    1010                 1015                 1020             


Leu Glu  Leu Ile Ser His Asp  Arg Asn Phe Ser Leu  Arg Gly Val 
    1025                 1030                 1035             


Gly Ser  Lys Thr Leu Lys Arg  Phe Glu Lys Tyr Gln  Val Asp Val 
    1040                 1045                 1050             


Leu Gly  Asn Ile His Lys Val  Lys Gly Glu Lys Arg  Val Gly Leu 
    1055                 1060                 1065             


Ala Ala  Pro Thr Asn Gln Lys  Lys Gly Lys Thr Val  Asp Ser Leu 
    1070                 1075                 1080             


Gln Ser  Val Ser Asp 
    1085             


<210>  625
<211>  1089
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  625

Met Thr Ala Phe Lys Pro Asn Pro Leu Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Asp Lys 
            20                  25                  30          


Glu Asn Pro Val Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Met Pro Lys Thr Gly Asp Ser Leu Ala Ala Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys His Glu Gly Val Leu Lys Pro Glu Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Val Lys Pro Leu Pro Tyr Ile Pro Lys Gln 
            100                 105                 110         


Pro Arg Asn Thr Pro Trp Gln Leu Arg Ala Ala Ala Leu Asp Arg Lys 
        115                 120                 125             


Leu Thr Pro Leu Glu Trp Ala Ala Val Leu Leu His Leu Val Lys His 
    130                 135                 140                 


Arg Gly Tyr Leu Ser Gln Arg Lys Asn Glu Gly Glu Thr Asp Asp Lys 
145                 150                 155                 160 


Glu Leu Gly Ala Leu Leu Lys Gly Val Ala Asp Asn Thr His Ala Leu 
                165                 170                 175     


Gln Ala Gly Asn Phe Arg Thr Pro Ala Glu Leu Ala Leu Asn Lys Phe 
            180                 185                 190         


Glu Lys Glu Ser Gly His Ile Arg Asn Gln Arg Gly Asp Tyr Ser His 
        195                 200                 205             


Thr Phe Ser Arg Lys Asp Leu Gln Ala Glu Leu Val Leu Leu Phe Glu 
    210                 215                 220                 


Lys Gln Lys Glu Phe Gly Asn Pro Tyr Val Ser Asp Asp Leu Lys Asn 
225                 230                 235                 240 


Gly Ile Glu Asn Leu Leu Met Thr Gln Arg Pro Ala Leu Ser Gly Asp 
                245                 250                 255     


Ala Ile Gln Lys Met Leu Gly His Cys Thr Phe Glu Pro Ala Glu Pro 
            260                 265                 270         


Lys Ala Ala Lys Asn Thr Tyr Thr Ala Glu Arg Phe Ile Trp Leu Thr 
        275                 280                 285             


Lys Leu Asn Asn Leu Arg Ile Leu Asn Gln Gly Asn Glu Arg Pro Leu 
    290                 295                 300                 


Thr Asp Ala Glu Arg Thr Thr Leu Met Asp Glu Pro Tyr Lys Lys Ser 
305                 310                 315                 320 


Lys Leu Thr Tyr Ala Gln Ala Arg Lys Leu Leu Asp Leu Asp Asp Thr 
                325                 330                 335     


Ala Phe Phe Lys Gly Leu Arg Tyr Gly Lys Asp Asn Ala Glu Ser Ala 
            340                 345                 350         


Thr Leu Met Glu Met Lys Ala Tyr His Ala Ile Ser Arg Ala Leu Glu 
        355                 360                 365             


Lys Glu Gly Leu Lys Asp Lys Lys Ser Pro Leu Ser Leu Ser Pro Glu 
    370                 375                 380                 


Leu Gln Asp Glu Ile Gly Thr Ala Phe Ser Leu Phe Lys Thr Asp Glu 
385                 390                 395                 400 


Glu Ile Thr Gly Arg Leu Lys Gly Cys Leu Gln Pro Glu Ile Leu Asp 
                405                 410                 415     


Ala Leu Leu Lys His Ile Ser Phe Asp Lys Phe Val Gln Ile Ser Leu 
            420                 425                 430         


Lys Ala Leu Arg Arg Ile Val Pro Leu Met Glu Gln Gly Lys Arg Tyr 
        435                 440                 445             


Asp Glu Ala Cys Ala Glu Ile Tyr Gly Asp His His Gly Arg Arg Asn 
    450                 455                 460                 


Ser Glu Glu Thr Leu Tyr Leu Pro Pro Ile Pro Ala Asp Glu Ile Arg 
465                 470                 475                 480 


Asn Pro Val Val Leu Arg Ala Leu Ser Gln Ala Arg Lys Val Ile Asn 
                485                 490                 495     


Gly Val Gly Arg Arg Tyr Gly Ser Pro Ala Arg Ile His Ile Glu Thr 
            500                 505                 510         


Ala Arg Glu Val Gly Lys Ser Phe Lys Asp Arg Lys Glu Ile Glu Lys 
        515                 520                 525             


Arg Gln Glu Glu Asn Arg Lys Asp Arg Glu Lys Ala Ala Ala Glu Phe 
    530                 535                 540                 


Gln Glu Tyr Phe Pro Asn Phe Val Gly Glu Pro Lys Ser Lys Asp Ile 
545                 550                 555                 560 


Leu Lys Leu Arg Leu Tyr Arg Gln Gln Asn Cys Lys Cys Leu Tyr Ser 
                565                 570                 575     


Gly Lys Glu Ile Asn Leu Gly Arg Leu Asn Glu Lys Gly Tyr Val Glu 
            580                 585                 590         


Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe Asn 
        595                 600                 605             


Asn Lys Val Leu Val Leu Gly Ser Glu Asn Gln Asn Lys Gly Asn Arg 
    610                 615                 620                 


Thr Pro Tyr Glu Tyr Leu Asn Gly Lys Asp Asn Ser Arg Glu Trp Gln 
625                 630                 635                 640 


Glu Phe Lys Ala Arg Val Glu Thr Ser Arg Phe Pro His Ser Lys Lys 
                645                 650                 655     


Gln Arg Ile Leu Leu Gln Lys Phe Asp Glu Glu Gly Phe Lys Glu Arg 
            660                 665                 670         


Asn Leu Asn Asp Thr Arg Tyr Val Asn Arg Phe Leu Cys Lys Phe Val 
        675                 680                 685             


Ala Asp His Met Gln Leu Thr Gly Lys Gly Lys Arg Arg Val Phe Ala 
    690                 695                 700                 


Ser Asn Gly Gln Ile Thr Asn Leu Leu Arg Gly Phe Trp Gly Leu Arg 
705                 710                 715                 720 


Lys Val Arg Ala Glu Asn Asp Arg His His Ala Leu Asp Ala Val Val 
                725                 730                 735     


Val Ala Cys Ser Thr Val Ala Met Gln Gln Arg Ile Thr Arg Phe Val 
            740                 745                 750         


Arg Tyr Lys Glu Met Asn Ala Phe Asp Gly Lys Thr Val Asp Lys Glu 
        755                 760                 765             


Thr Gly Glu Val Leu His Gln Lys Thr His Phe Pro Gln Pro Trp Glu 
    770                 775                 780                 


Phe Phe Ala Gln Glu Val Met Ile Arg Val Phe Gly Lys Pro Asp Gly 
785                 790                 795                 800 


Lys Pro Glu Leu Glu Glu Ala Asp Thr Pro Glu Lys Leu Arg Ala Leu 
                805                 810                 815     


Leu Ala Glu Lys Leu Ser Ser Arg Pro Glu Ala Val His Glu Tyr Val 
            820                 825                 830         


Ala Pro Leu Phe Val Ser Arg Ala Pro Asn Arg Lys Met Ser Gly Gln 
        835                 840                 845             


Gly His Met Glu Thr Ile Lys Ser Pro Lys Arg Leu Asp Glu Gly Ile 
    850                 855                 860                 


Ser Val Val Arg Val Pro Leu Thr Gln Leu Lys Leu Lys Asp Leu Glu 
865                 870                 875                 880 


Lys Met Val Asn Arg Glu Arg Glu Pro Lys Leu Tyr Glu Ala Leu Lys 
                885                 890                 895     


Ala Arg Leu Glu Ala His Lys Asp Asp Pro Ala Lys Ala Phe Ala Glu 
            900                 905                 910         


Pro Phe Tyr Lys Gln Asp Lys Ala Gly Asn Arg Thr Gln Gln Val Lys 
        915                 920                 925             


Ala Val Arg Ile Glu Gln Val Gln Lys Thr Gly Val Trp Val Arg Asn 
    930                 935                 940                 


Gln Asn Gly Ile Ala Asp Asn Ala Thr Met Val Arg Val Asp Val Phe 
945                 950                 955                 960 


Glu Lys Gly Gly Lys Tyr Tyr Leu Val Pro Ile Tyr Ser Trp Gln Val 
                965                 970                 975     


Ala Lys Gly Ile Leu Pro Asp Lys Ala Ile Ile Ala Phe Lys Asp Glu 
            980                 985                 990         


Glu Glu Trp Gln Leu Ile Asp Asp  Ser Phe Asn Phe Lys  Phe Pro Leu 
        995                 1000                 1005             


His Pro  Asn Asp Leu Val Glu  Val Ile Thr Lys Lys  Ala Arg Ile 
    1010                 1015                 1020             


Phe Gly  Tyr Phe Ala Ser Cys  His Arg Gly Thr Gly  Asn Ile Asn 
    1025                 1030                 1035             


Ile Arg  Ile His Asp Leu Asp  Asn Lys Val Gly Lys  Asn Gly Ile 
    1040                 1045                 1050             


Leu Glu  Gly Ile Gly Val Lys  Thr Ala Leu Ser Phe  Gln Lys Tyr 
    1055                 1060                 1065             


Gln Ile  Asp Glu Leu Gly Lys  Glu Ile Arg Pro Cys  Arg Leu Lys 
    1070                 1075                 1080             


Lys Arg  Pro Pro Val Arg 
    1085                 


<210>  626
<211>  1089
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  626

Met Asn Val Arg Tyr Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Asn Leu Asp Ile Pro Arg Ile Glu Asp Leu Gly 
            20                  25                  30          


Val Arg Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu 
        35                  40                  45              


Ala Leu Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg 
    50                  55                  60                  


Arg Lys His Arg Leu Glu Arg Ile Arg Arg Leu Leu Ile Arg Glu Gly 
65                  70                  75                  80  


Ile Leu Met Lys Glu Glu Leu Asp Lys Leu Phe Glu Glu Arg His Glu 
                85                  90                  95      


Ile Asp Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn 
            100                 105                 110         


Ser Asp Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly 
        115                 120                 125             


Phe Lys Ser Asn Arg Lys Ser Glu His Asn Ser Arg Glu Asn Ser Thr 
    130                 135                 140                 


Met Leu Lys His Ile Glu Glu Asn Arg Ala Ile Leu Ser Ser Tyr Arg 
145                 150                 155                 160 


Thr Val Ala Glu Met Ile Val Lys Asp Pro Lys Phe Ala Leu His Lys 
                165                 170                 175     


Arg Asn Lys Gly Glu His Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu 
            180                 185                 190         


Glu Arg Glu Ile Gln Leu Ile Phe Thr Lys Gln Arg Glu Phe Gly Asn 
        195                 200                 205             


Met Asn Cys Thr Lys Glu Phe Glu Asn Glu Tyr Ile Ala Ile Trp Ala 
    210                 215                 220                 


Ser Gln Arg Pro Phe Ala Ser Lys Asp Asp Ile Glu Lys Lys Val Gly 
225                 230                 235                 240 


Phe Cys Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr 
                245                 250                 255     


Thr Phe Gln Ser Phe Ile Ala Trp Glu His Ile Asn Lys Leu Arg Leu 
            260                 265                 270         


Val Ser Pro Ala Gly Thr Arg Gly Leu Thr Asp Glu Glu Arg Arg Leu 
        275                 280                 285             


Leu Tyr Glu Gln Ala Phe Gln Lys Asn Lys Ile Thr Tyr His Asp Ile 
    290                 295                 300                 


Arg Thr Leu Leu Arg Leu Pro Asp Asp Thr Tyr Phe Lys Gly Val Val 
305                 310                 315                 320 


Tyr Asp Arg Asn Met Pro Leu Lys Gln Asn Glu Thr Ile Arg Phe Leu 
                325                 330                 335     


Glu Leu Asp Ala Tyr His Gln Ile Arg Lys Val Ile Asp Lys Ala Tyr 
            340                 345                 350         


Gly Lys Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe 
        355                 360                 365             


Gly Tyr Ala Leu Thr Leu Phe Lys Asp Asp Ala Asp Val Arg Ser Tyr 
    370                 375                 380                 


Leu Gln Asn Glu Tyr Glu Gln Asn Gly Lys Pro Met Pro Asn Leu Ala 
385                 390                 395                 400 


Asn Lys Thr Tyr Asp Asn Glu Leu Ile Glu Glu Leu Leu Asn Leu Ser 
                405                 410                 415     


Phe Thr Lys Phe Gly His Leu Ser Leu Lys Ala Leu His Asn Ile Leu 
            420                 425                 430         


Pro Tyr Met Glu Gln Gly Glu Val Tyr Ser Ser Ala Cys Glu Arg Ala 
        435                 440                 445             


Gly Tyr Lys Phe Thr Gly Pro Arg Lys Lys Gln Lys Thr Met Leu Leu 
    450                 455                 460                 


Pro Asn Ile Pro Ser Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr 
465                 470                 475                 480 


Gln Ala Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro 
                485                 490                 495     


Val Ser Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp 
            500                 505                 510         


Glu Arg Arg Lys Thr Lys Arg Glu Gln Asp Glu Asn Arg Lys Lys Asn 
        515                 520                 525             


Glu Thr Ala Ile Arg Gln Leu Met Glu Tyr Gly Leu Thr Leu Asn Pro 
    530                 535                 540                 


Thr Gly His Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly 
545                 550                 555                 560 


Arg Cys Ala Tyr Ser Leu Gln Pro Ile Glu Ile Glu Arg Leu Leu Glu 
                565                 570                 575     


Pro Gly Tyr Thr Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu 
            580                 585                 590         


Asp Asp Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Lys Glu Asn Arg 
        595                 600                 605             


Glu Lys Gly Asn Arg Thr Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu 
    610                 615                 620                 


Arg Trp His Gln Phe Glu Thr Phe Val Leu Thr Asn Lys Gln Phe Ser 
625                 630                 635                 640 


Lys Lys Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu 
                645                 650                 655     


Glu Thr Glu Phe Lys Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser 
            660                 665                 670         


Arg Phe Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Glu Ser 
        675                 680                 685             


Asp Asp Lys Gln Lys Val Tyr Thr Val Asn Gly Arg Ile Thr Ala His 
    690                 695                 700                 


Leu Arg Ser Arg Trp Glu Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu 
705                 710                 715                 720 


His His Ala Val Asp Ala Ala Ile Val Ala Cys Thr Thr Pro Ser Asp 
                725                 730                 735     


Ile Ala Lys Ile Thr Ala Phe Tyr Gln Arg Arg Glu Gln Asn Lys Glu 
            740                 745                 750         


Leu Ala Lys Lys Thr Glu Pro His Phe Pro Gln Pro Trp Pro His Phe 
        755                 760                 765             


Ala Asp Glu Leu Arg Ala Arg Leu Ser Lys His Pro Lys Glu Ser Ile 
    770                 775                 780                 


Lys Ala Leu Asn Leu Gly Asn Tyr Asp Asn Glu Lys Leu Glu Ser Leu 
785                 790                 795                 800 


His Pro Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala 
                805                 810                 815     


Ala His Gln Glu Thr Leu Arg Arg Tyr Val Gly Ile Asp Glu Arg Ser 
            820                 825                 830         


Gly Lys Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu 
        835                 840                 845             


Asp Ala Ser Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Arg 
    850                 855                 860                 


Thr Tyr Glu Ala Ile Arg Gln Arg Leu Leu Glu His Asn Asn Asp Pro 
865                 870                 875                 880 


Lys Lys Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu 
                885                 890                 895     


Pro Gly Pro Ile Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Gln 
            900                 905                 910         


Val Val Pro Leu Asn Asp Gly Lys Thr Val Ala Tyr Asn Ser Asn Ile 
        915                 920                 925             


Val Arg Val Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro 
    930                 935                 940                 


Val Tyr Thr Met Asp Ile Met Lys Gly Val Leu Pro Asn Lys Ala Ile 
945                 950                 955                 960 


Glu Pro Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Asp Tyr 
                965                 970                 975     


Thr Phe Gln Phe Ser Leu Tyr Pro Asn Asp Leu Ile Arg Ile Glu Leu 
            980                 985                 990         


Pro Arg Glu Lys Ala Ile Arg Thr  Ser Thr Gly Glu Glu  Ile Lys Ile 
        995                 1000                 1005             


Lys Asp  Phe Phe Ala Tyr Tyr  Lys Thr Ile His Ser  Gly Thr Gly 
    1010                 1015                 1020             


Gly Met  Glu Leu Ile Ser His  Asp Arg Ser Phe Ser  Leu Ser Gly 
    1025                 1030                 1035             


Val Gly  Ser Arg Thr Leu Lys  Arg Phe Glu Lys Tyr  Gln Val Asp 
    1040                 1045                 1050             


Val Leu  Gly Asn Ile Tyr Lys  Val Arg Gly Glu Lys  Arg Val Gly 
    1055                 1060                 1065             


Leu Ala  Ser Ser Ala His Ser  Lys Thr Gly Lys Thr  Val Arg Pro 
    1070                 1075                 1080             


Leu Gln  Ser Thr Arg Asp 
    1085                 


<210>  627
<211>  1090
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  627

Met Arg Trp Arg Leu Gly Leu Asp Met Gly Ala Gly Ser Leu Gly Trp 
1               5                   10                  15      


Cys Ala Val Gly Leu Asp Asp Gln Gly Tyr Pro Asp Ser Ile Leu Ala 
            20                  25                  30          


Ala Gly Ser His Ile Phe Gly Asp Gly Arg Ala Pro Ser Arg Gly Asp 
        35                  40                  45              


Gln Gln Gly Thr Pro Leu Gly Val Glu Arg Arg Ala Ala Lys Ala Met 
    50                  55                  60                  


Arg Arg Arg Arg Asp Arg Phe Leu Gly Arg Gln Ala Lys Leu Leu Ala 
65                  70                  75                  80  


Arg Leu Thr Leu Ala Gly Leu Phe Pro Ala Asp Pro Val Arg Ala Lys 
                85                  90                  95      


Ala Leu Glu Leu Leu Asp Pro Tyr Val Leu Arg Ala Arg Ala Leu Asp 
            100                 105                 110         


Glu Ala Leu Pro Pro His Gln Leu Gly Arg Ala Phe Phe His Leu Asn 
        115                 120                 125             


Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Arg Gly Val Asp 
    130                 135                 140                 


Asp Glu Ala Gly Lys Ile Ala Ile Gly Ile Asp Arg Leu Arg Ala Ala 
145                 150                 155                 160 


Ile Ala Ala Ala Glu Ala Arg Thr Met Gly Glu Phe Leu His Lys Arg 
                165                 170                 175     


Arg Gln Ser Ala Pro Asp Val Asn Gln Ile Pro Ser Val Arg Thr Arg 
            180                 185                 190         


Leu Arg Pro Glu Thr Gly Glu Glu Ala Lys Gly Asp Gly Tyr Asp Phe 
        195                 200                 205             


Tyr Pro Gly Arg Glu Leu Val Glu Glu Glu Phe Asp Ala Ile Trp Asp 
    210                 215                 220                 


Ala Gln Ala Pro His His Pro Ser Leu Leu Thr Pro Asp Asn Arg Ala 
225                 230                 235                 240 


Leu Leu His Glu Ala Ile Phe Tyr Gln Arg Pro Leu Lys Ala Pro Lys 
                245                 250                 255     


Ile Gly Lys Cys Thr Leu Leu Asn Asn Glu Pro Arg Leu Pro Lys Ala 
            260                 265                 270         


His Pro Leu Phe Gln Arg Arg Arg Leu Leu Glu Glu Val Asn Ala Leu 
        275                 280                 285             


Arg Ile Val Arg Pro Gly Glu Val Ala Thr Lys Leu Thr Cys Glu Gln 
    290                 295                 300                 


Arg Asp Leu Leu Met Leu Lys Leu Lys Asp Lys Ala Lys Val Ser Phe 
305                 310                 315                 320 


Glu Thr Leu Arg Ser Lys Val Leu Lys Leu Glu Pro Glu Ala Arg Phe 
                325                 330                 335     


Asn Lys Glu Gly Glu Asn Arg Thr Glu Leu Val Gly Asp Glu Val Cys 
            340                 345                 350         


Ala Gln Met Ser Ala Lys Thr Arg Phe Gly Lys Ala Trp Ala His Phe 
        355                 360                 365             


Thr Asp Glu Lys Arg Trp Ala Ile Leu Ser Arg Leu Ile Glu Glu Glu 
    370                 375                 380                 


Asp Glu Glu Arg Leu Asp Thr Trp Leu Arg Thr Glu His Gly Leu Asp 
385                 390                 395                 400 


Ala Glu Gln Ala Arg Ala Val Ala Gly Ala Arg Leu Pro Ala Gly Phe 
                405                 410                 415     


Gly Arg Phe Gly Glu Thr Ala Thr Arg Arg Leu Ile Ala Ala Leu Gln 
            420                 425                 430         


Ala Glu Val Ile Val Tyr Ser Glu Ala Val Val Ala Ala Gly Leu Gly 
        435                 440                 445             


His His Ser Asp Met Arg Thr Gly Glu Val Leu Ala Glu Leu Pro Tyr 
    450                 455                 460                 


Tyr Gly Met Thr Leu Glu Arg His Leu Leu Pro Gly Thr Gly Asp Lys 
465                 470                 475                 480 


Ala Asp Pro Glu Glu Leu Arg Ile Gly Arg Leu Thr Asn Pro Thr Val 
                485                 490                 495     


His Ile Ala Leu Asn Arg Leu Arg Arg Ile Val Asn Arg Leu Ile Lys 
            500                 505                 510         


Thr Tyr Gly Arg Pro Glu Gln Ile Ala Ile Glu Leu Gly Arg Glu Leu 
        515                 520                 525             


Lys Leu Thr Asp Glu Asp Lys Gln Arg Leu Ser Arg Gln Asn Asn Arg 
    530                 535                 540                 


Asn Arg Ala Asp Ala Glu Ala Arg Ser Lys Lys Leu Ala Glu Leu Gly 
545                 550                 555                 560 


Gln Ala Asp Asn Gly Ala Asn Arg Ala Arg Leu Lys Leu Trp Glu Glu 
                565                 570                 575     


Leu Asn Arg Glu Asn Ala Leu Asp Arg Arg Cys Ile Tyr Ser Gly Gln 
            580                 585                 590         


Gln Ile Ser Ile Ala Met Leu Phe Ser Asp Ala Val Glu Val Asp His 
        595                 600                 605             


Ile Leu Pro Phe Asp Ala Thr Leu Asp Asp Ser Asn Asn Asn Arg Ile 
    610                 615                 620                 


Leu Cys Leu Arg Glu Trp Asn Arg Arg Lys Arg Lys Gln Ser Pro Tyr 
625                 630                 635                 640 


Glu Ala Trp Gly His Thr Asp Tyr Trp Pro Asp Ile Ala Ala Arg Ala 
                645                 650                 655     


Ala Arg Leu Pro Lys Glu Lys Arg Trp Arg Phe Glu Pro Asp Ala Met 
            660                 665                 670         


Gln Arg Phe Ser Ala Glu Asp Arg Phe Ile Ala Arg His Leu Val Asp 
        675                 680                 685             


Thr Gln Tyr Met Ser Arg Leu Ala Arg Glu Tyr Leu Glu Ala Leu Tyr 
    690                 695                 700                 


Pro Glu Lys Gly Glu Gly Ser Gly His Val Trp Val Ser Pro Gly Arg 
705                 710                 715                 720 


Leu Thr Glu Leu Ile Arg Arg Lys Leu Gly Leu Asn Glu Leu Leu Pro 
                725                 730                 735     


Asp His Asn Phe Ala Gly Val Lys Asn Arg Leu Asp His Arg His His 
            740                 745                 750         


Thr Ile Asp Ala Phe Val Val Ala Val Thr Asp Arg Ser Leu Leu Gln 
        755                 760                 765             


Gly Ile Ser Arg Ala Ser Ala Leu Glu Gly Glu Glu Gly Arg Glu Arg 
    770                 775                 780                 


Val Ile Val Pro Glu Pro Trp Asp Gly Phe Arg Glu Ala Leu Arg Thr 
785                 790                 795                 800 


Thr Leu Asn Thr Ile Thr Val Trp His Arg Gln Asp His Gly Thr Ala 
                805                 810                 815     


Ser Lys Ala Gly Leu Ala Gln Gly Arg Asp Gln Thr Ala Gly Lys Leu 
            820                 825                 830         


His Asn Asp Thr Ala Tyr Gly Leu Thr Gly Arg Gln Asp Thr Lys Gly 
        835                 840                 845             


Asn Asp Leu Val Val Ser Arg Lys Pro Leu Met Ser Leu Lys Pro Ala 
    850                 855                 860                 


Asp Ile Glu Pro Val Pro Asp Val Ala Pro Ile Thr Lys Gly Val Arg 
865                 870                 875                 880 


Asp Arg Asp Leu Arg Lys Ala Leu Ala His Phe Thr Gln Ala Thr Glu 
                885                 890                 895     


Gly Lys Gly Phe Leu Gln Ala Leu Glu Arg Phe Thr Arg Leu Gly Pro 
            900                 905                 910         


Leu His Phe Arg Gly Ile Arg Arg Val Arg Leu Ile Gln Pro Leu Ala 
        915                 920                 925             


Val Ile Pro Ile Lys Gly Pro Gly Gly Val Pro Tyr Lys Gly Tyr Leu 
    930                 935                 940                 


Gly Gly Glu Asn Phe Arg Cys Asp Val Trp Glu Met Pro Asp Gly Ala 
945                 950                 955                 960 


Trp Lys Pro Glu Val Thr Ser Met Phe Gln Ala His Gln Pro Gly Trp 
                965                 970                 975     


Thr Ser Ala Ile Arg Ala Ala Cys Pro Thr Ala Arg Lys Val Leu Ser 
            980                 985                 990         


Leu His Lys Arg Asp Met Ile Ala  Ile Glu Arg Glu Glu  Gly Val Arg 
        995                 1000                 1005             


Thr Leu  Met Val Val Ala Gln  Ile Trp Ser Asn Gly  Gln Val Thr 
    1010                 1015                 1020             


Leu Val  Glu Pro His Glu Ala  Gly Asp Leu Asn Lys  Arg Asn Lys 
    1025                 1030                 1035             


Ser Glu  Asp Asp Pro Phe Gly  Tyr Phe Met Pro Thr  Ala Gly Gly 
    1040                 1045                 1050             


Leu Lys  Arg Leu Lys Ala Arg  Gln Val Arg Val Asp  Glu Leu Gly 
    1055                 1060                 1065             


Arg Val  Gln Asp Pro Gly Phe  Pro Ala Arg Lys Ala  Arg Arg Arg 
    1070                 1075                 1080             


Thr Arg  Pro Lys Gln Val Asp  
    1085                 1090 


<210>  628
<211>  1090
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  628

Met Asp Trp Arg Leu Gly Leu Asp Leu Gly Ala Asn Ser Leu Gly Trp 
1               5                   10                  15      


Ala Ala Leu Arg Leu Asp Arg Ala Gly Asp Pro Ile Gly Ile Leu Ala 
            20                  25                  30          


Met Gly Ser Arg Ile Phe Ser Asp Gly Arg Asp Pro Lys Ser Gly Ala 
        35                  40                  45              


Ser Leu Ala Val Asp Arg Arg Ala Ala Arg Ala Met Arg Arg Arg Arg 
    50                  55                  60                  


Asp Arg Phe Lys Gln Arg Gln Ala Ala Leu Leu Lys Tyr Leu Ile Arg 
65                  70                  75                  80  


Asp Gly Leu Phe Pro Ala Asp Gln Ala Asp Arg Gln Arg Leu Ala Asp 
                85                  90                  95      


Glu Asp Pro Tyr Ala Leu Arg Ala Arg Ala Leu Asp Glu Glu Leu Ser 
            100                 105                 110         


Leu Phe Glu Ile Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly 
        115                 120                 125             


Phe Gln Ser Asn Arg Lys Ala Asp Arg Gly Gln Asp Asp Glu Ala Gly 
    130                 135                 140                 


Lys Ile Ala Val Gly Val Asp Arg Leu Asn Gln Gln Met Glu Glu Gln 
145                 150                 155                 160 


Gly Ala Arg Thr Leu Gly Glu Phe Leu His Gln Arg Arg Arg Ala Ala 
                165                 170                 175     


Thr Asp Ala Asn His Ile Pro Ser Val Arg Thr Arg Leu Arg Pro Glu 
            180                 185                 190         


Thr Gly Glu Gly Ala Lys Gly Asp Gly Tyr Asp Phe Tyr Pro Ser Arg 
        195                 200                 205             


Ala Leu Ile Ala Glu Glu Phe Glu Glu Ile Trp Ala Ala Gln Ala Pro 
    210                 215                 220                 


His Tyr Pro Asp Thr Leu Thr Gln Gln Val His Asp Arg Leu Tyr Glu 
225                 230                 235                 240 


Ile Ile Phe His Gln Arg Pro Leu Arg Ala Pro Gln Val Gly Gln Cys 
                245                 250                 255     


Thr Leu Val Ala Gly Glu Thr Arg Leu Pro Lys Ala His Pro Leu Phe 
            260                 265                 270         


Gln Lys Arg Arg Leu Leu Glu Glu Ile Asn Ala Leu Met Ile Val Arg 
        275                 280                 285             


Pro Gly Glu Ile Ala Gln Arg Leu Thr Pro Glu Glu Arg Asp Thr Leu 
    290                 295                 300                 


Tyr Leu Lys Leu Lys Asp Lys Arg Thr Val Ser Phe Ala Thr Leu Arg 
305                 310                 315                 320 


Lys Thr Leu Lys Leu Asp Ser Ala Ala Arg Phe Asn Lys Glu Ser Glu 
                325                 330                 335     


Asn Arg Thr Glu Leu Lys Gly Asp Glu Val Ala Ala Glu Leu Gly Ala 
            340                 345                 350         


Lys Thr Arg Leu Gly Thr Arg Trp Gln His Leu Ser Gly Asp Gln Gln 
        355                 360                 365             


Trp Glu Val Val His Arg Leu Met Ala Leu Glu Ser Gln Ala Asp Glu 
    370                 375                 380                 


Ala Ala Phe Arg Asp Trp Ile Ser Gln Thr Tyr Asp Leu Leu Pro Glu 
385                 390                 395                 400 


Gln Ala Ala Ala Val Leu Gly Ala Arg Leu Pro Gln Gly His Gly Arg 
                405                 410                 415     


Phe Gly Pro Thr Ala Thr Thr Thr Leu Ile Asp Ser Leu Leu His Asp 
            420                 425                 430         


Arg Thr Glu Asp Gly Arg Val Val Val Tyr Ser Glu Ala Val Gln Arg 
        435                 440                 445             


Ser Gly Leu Gly His His Ser Asp Leu Arg His Asp Arg Asp Asp Glu 
    450                 455                 460                 


Asp Val Thr Glu Leu Pro Tyr Tyr Gly Val Ala Leu Glu Arg His Ile 
465                 470                 475                 480 


Met Pro Gly Thr Ala Glu Pro Thr Asp Pro Glu Glu Met Arg Ile Gly 
                485                 490                 495     


Arg Leu Thr Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu Arg His 
            500                 505                 510         


Val Val Asn Arg Leu Ile Arg Ala Tyr Gly Pro Pro Lys Glu Ile Ala 
        515                 520                 525             


Ile Glu Leu Ala Arg Glu Leu Lys Leu Ser Glu Asp Glu Lys Lys Lys 
    530                 535                 540                 


Arg Asn Ser Glu Asn Arg Asp Asn Arg Leu Ala Ala Glu Gly Arg Ser 
545                 550                 555                 560 


Lys Lys Leu Glu Glu Ile Arg Gln Arg Asp Thr Gly Ala Asn Arg Ala 
                565                 570                 575     


Leu Leu Lys Leu Trp Glu Glu Leu Asn Pro Gly Asn Val Leu Asp Arg 
            580                 585                 590         


Arg Cys Ile Tyr Thr Gly Glu Gln Ile Ser Ile Asp Met Leu Phe Ser 
        595                 600                 605             


Asp Arg Val Glu Val Asp His Ile Leu Pro Phe Ala Gln Thr Leu Asp 
    610                 615                 620                 


Asp Ser Asn Gly Asn Lys Leu Leu Cys Leu Arg Glu Ala Asn Arg Glu 
625                 630                 635                 640 


Lys Arg Arg Arg Ser Pro Tyr Glu Ala Trp Gly His Thr Asp Arg Trp 
                645                 650                 655     


Pro Asp Ile Ala Glu Arg Ala Ser Arg Leu Pro Lys Pro Lys Arg Trp 
            660                 665                 670         


Arg Phe Glu Pro Asp Ala Met Gln Arg Phe Ala Thr Glu Asp Gly Phe 
        675                 680                 685             


Leu Ala Arg His Leu Val Asp Thr Gln Tyr Leu Gly Arg Leu Ala Arg 
    690                 695                 700                 


Glu Tyr Leu Ser Trp Leu Tyr Pro Gly Arg Gly Glu Gly Ser Gly His 
705                 710                 715                 720 


Val Trp Val Ser Pro Gly Arg Leu Thr Glu Met Val Arg Arg Lys Leu 
                725                 730                 735     


Gly Leu Asn Asp Leu Leu Pro Asp His Asn Phe Gly Gly Gly Ala Asp 
            740                 745                 750         


Gln Pro Lys Asn Arg Leu Asp His Arg His His Ala Ile Asp Ala Leu 
        755                 760                 765             


Val Val Ala Ile Val Asp Arg Gly Met Leu Gln Ala Ile Ala Arg Ala 
    770                 775                 780                 


Ser Gly Gln Asp Gly Ser Glu Gly Arg Glu Arg Ile His Ile Pro Ala 
785                 790                 795                 800 


Pro Trp Asp Gly Phe Arg Asp Asp Leu Arg Val Ala Val Asn Gly Ile 
                805                 810                 815     


Ile Val Ser His Arg Pro Asp His Gly Thr Val Ala Lys Ala Gly Leu 
            820                 825                 830         


Ala Arg Gly Arg Asp Gln Thr Ala Gly Arg Leu His Asn Asp Thr Ala 
        835                 840                 845             


Tyr Gly Leu Thr Gly Glu Thr Asp Ala Lys Gly Asn Ser Ile Val Val 
    850                 855                 860                 


Arg Arg Ile Pro Leu Ser Ala Ile Lys Lys Pro Ala Asp Ile Ala Arg 
865                 870                 875                 880 


Val Arg Asp Pro Leu Leu Arg Asn Ala Leu Ala Glu Phe Thr Lys Gly 
                885                 890                 895     


Leu Asp Gly Lys Ala Phe Glu Ala Arg Ile Gly Arg Phe His Glu Leu 
            900                 905                 910         


Gly Pro Leu Leu Tyr Arg Gly Ile Arg Arg Val Arg Val Val Glu Thr 
        915                 920                 925             


Leu Thr Val Ile Pro Ile Arg Asp Ala Glu Gly Arg Ala Tyr Lys Gly 
    930                 935                 940                 


Tyr Lys Gly Asp Ser Asn Tyr Arg Tyr Asp Val Trp Glu Met Pro Asp 
945                 950                 955                 960 


Gly Lys Trp Arg Cys Gln Val Val Ser Met Phe Asp Ala His Gln Ser 
                965                 970                 975     


Thr Glu Gln Ala Arg Pro His Pro Ala Ala Arg Lys Ile Leu Ser Leu 
            980                 985                 990         


Gln Arg Asn Asp Ile Leu Ala Ile  Glu Arg Thr Glu Gly  Asp Arg Glu 
        995                 1000                 1005             


Leu Val  Arg Val Val Lys Phe  Ser Glu Lys Gln Phe  Ala Val Ala 
    1010                 1015                 1020             


Pro Pro  His Glu Gly Gly Ser  Leu Lys Thr Arg Asp  Gly Asn Ala 
    1025                 1030                 1035             


Asp Asp  Pro Phe Arg Tyr Leu  Tyr Pro Ser Pro Asn  Thr Leu Lys 
    1040                 1045                 1050             


Gly Trp  Arg Ala Arg Gln Val  Arg Ile Asp Glu Leu  Gly Arg Val 
    1055                 1060                 1065             


Leu Asp  Pro Gly Phe Pro Ala  Arg Lys Ser Val Arg  Arg Thr Arg 
    1070                 1075                 1080             


Pro Arg  Ala Ala Pro Val Asp  
    1085                 1090 


<210>  629
<211>  1091
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  629

Met Tyr Ser Asn Leu Leu Phe Ser Phe Asp Ile Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Trp Cys Val Phe Ala Leu Asp Glu Ala Gly Glu Pro Tyr Arg Ile 
            20                  25                  30          


Val Asp Leu Gly Ala Arg Ile Tyr Ala Asp Gly Arg Asp Pro Gln Ser 
        35                  40                  45              


Lys Thr Ser Leu Ala Val Ala Arg Arg Glu Ala Arg Ala Met Ser Arg 
    50                  55                  60                  


Arg Arg Asp Arg Tyr Leu Arg Arg Arg Lys Ala Thr Leu Arg Thr Met 
65                  70                  75                  80  


Ile Glu Tyr Gly Leu Met Pro Gly Asp Lys Ala Gly Gln Glu Ala Val 
                85                  90                  95      


Leu Arg Glu Thr Gly Asp Arg Glu Gly Gly Asp Glu Ser Phe Asn Pro 
            100                 105                 110         


Tyr Ala Leu Arg Ala Arg Ala Leu Ser Glu Lys Leu Pro Pro Ala Tyr 
        115                 120                 125             


Ile Gly Arg Val Leu Phe His Leu Gly Gln Arg Arg Gly Phe Lys Ser 
    130                 135                 140                 


Asn Arg Lys Thr Asp Arg Lys Asp Asn Asp Lys Gly Lys Ile Ala Leu 
145                 150                 155                 160 


Gly Ile Asp Glu Leu Thr Ala Ala Met His Arg Ser Gly Ser Pro Thr 
                165                 170                 175     


Leu Gly Ala Trp Leu Ala Met Arg Arg Ala Glu Gly His Ser Val Arg 
            180                 185                 190         


Leu Arg Ala Gly Ser Glu Val Phe Asp Ala Glu Gly Tyr Ala Phe Tyr 
        195                 200                 205             


Pro Glu Arg Ser Leu Leu Glu Asp Glu Phe Arg Gln Ile Trp Asp Ala 
    210                 215                 220                 


Gln Ala Val His His Pro Asp Ile Leu Thr Pro Glu Arg Arg Ala His 
225                 230                 235                 240 


Leu Phe Arg Val Met Phe Tyr Gln Arg Pro Leu Lys Lys Pro Leu Val 
                245                 250                 255     


Gly Arg Cys Ser Phe Asn Pro Ala Glu Phe Arg Leu Ser Arg Ala His 
            260                 265                 270         


Pro Leu Phe Gln Glu Phe Arg Leu Tyr Lys Glu Ile Asn Asp Leu Glu 
        275                 280                 285             


Val Val Leu Pro Asp Gln Ser His Arg Lys Leu Thr Leu Asp Glu Arg 
    290                 295                 300                 


Asn Ala Leu Val Ala Lys Leu Lys Thr Ser Arg Lys Ala Ser Phe Ser 
305                 310                 315                 320 


Ala Leu Arg Lys Thr Leu Lys Leu Thr Pro Asp Leu Val Phe Asn Lys 
                325                 330                 335     


Glu Ser Glu Ala Arg Lys Glu Leu Leu Gly Asp Glu Ile Tyr Ser Val 
            340                 345                 350         


Leu Ala Asp Ala Lys Met Phe Gly Ala Arg Trp Gly Gly Phe Ser Arg 
        355                 360                 365             


Ala Arg Gln Trp Glu Ile Ile Thr Arg Leu Lys Glu Glu Glu Asp Pro 
    370                 375                 380                 


Ala Ser Leu Ser Ser Trp Leu Lys Ser Glu Phe Gly Leu Asp Asp Glu 
385                 390                 395                 400 


Arg Val Thr Ala Ile Ala Asn Ala Ser Leu Pro Asp Gly Tyr Gly Arg 
                405                 410                 415     


Leu Gly Glu Thr Ala Leu Ala Ser Met Leu Glu Glu Leu Lys Thr Ala 
            420                 425                 430         


Val Ile Pro Glu Ser Glu Ala Ala Arg Leu Cys Gly Tyr Asp His Ser 
        435                 440                 445             


Asn Leu Ala Lys Glu Gln Asp Glu Gly Leu Asp Thr Leu Pro Glu Tyr 
    450                 455                 460                 


Gln Glu Ile Leu Glu Arg His Ile Pro Pro Gly Thr Gly Asp Pro Asp 
465                 470                 475                 480 


Asp Ile Tyr Asp Leu Arg Lys Gly Arg Ile Thr Asn Pro Thr Val His 
                485                 490                 495     


Ile Gly Leu Asn Gln Leu Arg Arg Val Val Asn Ala Leu Ile His Arg 
            500                 505                 510         


Phe Gly Arg Pro Gln Arg Ile Val Val Glu Leu Ala Arg Asp Leu Gln 
        515                 520                 525             


Leu Ser Glu Lys Gln Lys Asp Asp Val Asn Arg Arg Ile Ala Lys Asn 
    530                 535                 540                 


Thr Arg Glu Ala Glu Gly Arg Ser Gln Lys Leu Ile Glu Met Gly Gln 
545                 550                 555                 560 


Leu Asp Thr Gly Tyr Asn Arg Leu Leu Leu Lys Leu Trp Glu Glu Leu 
                565                 570                 575     


Asn Leu Asp Lys Pro Glu Asp Arg Val Cys Ile Tyr Ser Gly Lys Pro 
            580                 585                 590         


Ile Gly Ile Ala Met Leu Phe Ser Gly Glu Val Asp Val Asp His Ile 
        595                 600                 605             


Leu Pro Trp Ser Lys Thr Leu Asp Asp Ser Gln Ala Asn Lys Leu Leu 
    610                 615                 620                 


Cys Leu Lys Ser Ala Asn Arg Gln Lys Arg Asn Arg Thr Pro Ser Asp 
625                 630                 635                 640 


Val Pro Glu Trp Arg Asp Arg Tyr Asp Glu Val Leu Ala Arg Ala Ala 
                645                 650                 655     


Arg Leu Pro Arg Asn Lys Arg Trp Arg Phe Ala Ala Asp Ala Met Gln 
            660                 665                 670         


Gln Phe Glu Ala Glu Gly Gly Phe Leu Ala Arg Gln Leu Thr Asp Thr 
        675                 680                 685             


Gln Tyr Leu Ser Arg Met Ala Leu Glu Tyr Leu Ser Ala Leu Tyr Pro 
    690                 695                 700                 


Ser Glu Glu Ala Asp Lys Ser Gly Glu Leu Arg Gln Arg Lys Arg Val 
705                 710                 715                 720 


His Val Val Pro Gly Arg Leu Thr Glu Leu Leu Arg Arg Asn Trp Gly 
                725                 730                 735     


Leu Asn Thr Leu Leu Pro Asp His Asn Leu Gly Glu Met Ala Gln Glu 
            740                 745                 750         


Lys Asn Arg Arg Asp His Arg His His Ala Ile Asp Ala Ala Val Ile 
        755                 760                 765             


Gly Thr Thr Ser Arg Ser Leu Leu Gln Arg Met Ser Gly Ala Ala Ala 
    770                 775                 780                 


Arg Leu Asp Asp Val Ala Phe Asp Asp Leu Val Arg Thr Val Val Lys 
785                 790                 795                 800 


Asp Asn Pro Pro Trp Pro Gly Phe Arg Glu Glu Leu Leu Ala Ala Ile 
                805                 810                 815     


Gly Arg Val Thr Val Ser His Lys Pro Asp His Gly Thr Val Ser Arg 
            820                 825                 830         


Ala Ala Tyr Ala Gln Gly Lys Gly Gln Thr Ala Gly Lys Leu His Asn 
        835                 840                 845             


Asp Thr Ala Tyr Gly Leu Thr Gly Leu Val Asp Ala Lys Gly Ser Pro 
    850                 855                 860                 


Leu Val Val Arg Arg Lys Pro Phe Met Ala Leu Glu Val Lys Asp Ile 
865                 870                 875                 880 


Ala Ser Ile Arg Asp Thr Glu Leu Gln Ser Ala Leu Tyr Gly Ala Ile 
                885                 890                 895     


Gly Ser Leu Thr Glu Lys Lys Ala Leu Gln Glu Ala Leu Ile Arg Phe 
            900                 905                 910         


Arg Asp Thr His Pro Gln Phe Lys Gly Ile Arg Arg Val Arg Val Leu 
        915                 920                 925             


Glu Thr Leu Ser Val Ile Pro Ile Val Asp Arg Asp Gly Lys Ala Tyr 
    930                 935                 940                 


Lys Gly Tyr Lys Gly Asp Ala Asn Tyr Arg Tyr Glu Val Trp Glu Thr 
945                 950                 955                 960 


Leu Asp Gly Lys Trp His Thr Glu Val Val Ser Met Phe Asp Ala His 
                965                 970                 975     


Gln Pro Gly Trp Gln Ser Pro Phe His Lys Gln Asn Pro Ala Ala Arg 
            980                 985                 990         


Arg Val Leu Lys Leu Gln Gln Asn  Asp Met Val Ala Tyr  Glu His Pro 
        995                 1000                 1005             


Ala Asp  Gly Tyr Thr Ile Ala  Arg Val Val Lys Phe  Ser Asn Asp 
    1010                 1015                 1020             


Lys Arg  Ile Tyr Phe Ala Ser  His Arg Glu Ser Gly  Ser Leu Lys 
    1025                 1030                 1035             


Ala Arg  Asp Ala Asp Lys Gly  Asp Pro Phe Ser Tyr  Phe Ala Lys 
    1040                 1045                 1050             


Ala Asn  Asn Gly Leu Arg Asp  Ile Lys Cys Arg Gln  Val Arg Ile 
    1055                 1060                 1065             


Asp Ala  Ala Gly Arg Ile Phe  Asp Pro Gly Pro Gln  Asp Arg Glu 
    1070                 1075                 1080             


Thr Arg  Gln Ala Asn Lys Gly  Val 
    1085                 1090     


<210>  630
<211>  1092
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  630

Met Ala Tyr Thr Met Gly Ile Asp Val Gly Ile Ala Ser Cys Gly Trp 
1               5                   10                  15      


Ala Ile Val Asp Leu Glu Arg Gln Arg Ile Ile Asp Ile Gly Val Arg 
            20                  25                  30          


Thr Phe Glu Lys Ala Glu Asn Pro Lys Asn Gly Glu Ala Leu Ala Val 
        35                  40                  45              


Pro Arg Arg Glu Ala Arg Ser Ser Arg Arg Arg Leu Arg Arg Lys Lys 
    50                  55                  60                  


His Arg Ile Glu Arg Leu Lys Tyr Met Phe Val Arg Asn Gly Leu Ala 
65                  70                  75                  80  


Val Asp Ile Gln His Leu Glu Gln Thr Leu Arg Ser Gln Asn Glu Ile 
                85                  90                  95      


Asp Val Trp Gln Leu Arg Val Asp Gly Leu Asp Arg Met Leu Thr Gln 
            100                 105                 110         


Lys Glu Trp Leu Arg Val Leu Ile His Leu Ala Gln Arg Arg Gly Phe 
        115                 120                 125             


Gln Ser Asn Arg Lys Thr Asp Gly Ser Ser Glu Asp Gly Gln Val Leu 
    130                 135                 140                 


Val Asn Val Thr Glu Asn Asp Arg Leu Met Glu Glu Lys Asp Tyr Arg 
145                 150                 155                 160 


Thr Val Ala Glu Met Met Val Lys Asp Glu Lys Phe Ser Asp His Lys 
                165                 170                 175     


Arg Asn Lys Asn Gly Asn Tyr His Gly Val Val Ser Arg Ala Ser Leu 
            180                 185                 190         


Leu Val Glu Ile His Thr Leu Phe Glu Ser Gln Arg Gln His His Asn 
        195                 200                 205             


Phe Leu Ala Ser Lys Asp Phe Glu Leu Glu Tyr Ile Asn Ile Trp Ser 
    210                 215                 220                 


Ser Gln Arg Pro Val Ala Thr Lys Asp Gln Ile Glu Lys Met Ile Gly 
225                 230                 235                 240 


Thr Cys Thr Phe Leu Pro Lys Glu Lys Arg Ala Pro Lys Ala Ser Trp 
                245                 250                 255     


His Phe Gln Tyr Phe Met Leu Leu Gln Thr Ile Asn His Ile Arg Ile 
            260                 265                 270         


Thr Asn Ala Gln Gly Thr Arg Pro Leu Asn Lys Glu Glu Ile Glu Gln 
        275                 280                 285             


Val Val Asn Met Ala Leu Thr Lys Ser Lys Val Ser Tyr His Asp Ile 
    290                 295                 300                 


Arg Lys Val Leu Asp Leu Ser Glu Glu Tyr Gln Phe Val Gly Leu Asp 
305                 310                 315                 320 


Tyr Gly Lys Glu Asp Glu Lys Lys Lys Val Glu Ser Lys Glu Thr Ile 
                325                 330                 335     


Ile Lys Leu Asp Asp Tyr His Lys Leu Asn Lys Ile Phe Asn Glu Val 
            340                 345                 350         


Glu Leu Ala Lys Gly Glu Thr Trp Glu Ala Val Asp Tyr Asp Thr Val 
        355                 360                 365             


Ala Tyr Ala Leu Thr Phe Phe Lys Asp Asp Glu Asp Ile Arg Asp Tyr 
    370                 375                 380                 


Leu Gln Asn Lys Tyr Lys Asp Ser Lys Asn Arg Leu Val Lys Asn Val 
385                 390                 395                 400 


Ala Asn Lys Glu Tyr Thr Asn Glu Leu Ile Glu Lys Val Ser Thr Leu 
                405                 410                 415     


Ser Phe Arg Lys Val Gly His Leu Ser Leu Lys Ala Leu Arg Lys Ile 
            420                 425                 430         


Ile Pro Phe Leu Glu Gln Gly Met Thr Tyr Asp Lys Ala Cys Gln Ala 
        435                 440                 445             


Ala Gly Phe Asn Phe Gln Gly Ile Ser Lys Lys Lys Arg Ser Val Val 
    450                 455                 460                 


Leu Pro Val Ile Asp Gln Ile Ser Asn Pro Val Val Asn Arg Ala Leu 
465                 470                 475                 480 


Thr Gln Thr Arg Lys Val Ile Asn Ala Leu Ile Lys Lys Tyr Gly Ser 
                485                 490                 495     


Pro Glu Thr Ile His Ile Glu Thr Ala Arg Glu Leu Ser Lys Arg Phe 
            500                 505                 510         


Asp Glu Arg Lys Asn Ile Thr Lys Asp Tyr Lys Glu Asn Arg Asp Lys 
        515                 520                 525             


Asn Glu Arg Ala Lys Lys His Leu Ser Glu Leu Gly Ile Ile Asn Pro 
    530                 535                 540                 


Thr Gly Leu Asp Ile Val Lys Tyr Lys Leu Trp Cys Glu Gln Gln Gly 
545                 550                 555                 560 


Arg Cys Met Tyr Ser Asn Gln Pro Ile Ser Phe Glu Arg Leu Arg Glu 
                565                 570                 575     


Ser Gly Tyr Thr Glu Val Asp His Ile Ile Pro Tyr Ser Arg Ser Met 
            580                 585                 590         


Asn Asp Ser Tyr Asn Asn Arg Val Leu Val Met Thr Arg Glu Asn Arg 
        595                 600                 605             


Glu Lys Ser Asn Gln Thr Pro Tyr Glu Tyr Met Gly Asn Asp Thr Gln 
    610                 615                 620                 


Arg Trp Asp Glu Phe Glu Gln Arg Val Thr Thr Asn Pro Gln Ile Lys 
625                 630                 635                 640 


Lys Glu Lys Arg Gln Asn Leu Leu Leu Lys Gly Phe Thr Asn Arg Arg 
                645                 650                 655     


Glu Leu Glu Met Leu Glu Arg Asn Leu Asn Asp Thr Arg His Ile Thr 
            660                 665                 670         


Lys Tyr Leu Ser His Phe Ile Ser Thr Asn Leu Glu Phe Ser Pro Ser 
        675                 680                 685             


Asp Lys Lys Lys Lys Val Val Asn Thr Ser Gly Arg Ile Thr Ser His 
    690                 695                 700                 


Leu Arg Ser Arg Trp Gly Leu Glu Lys Asn Arg Glu Gln Asn Asp Leu 
705                 710                 715                 720 


His His Ala Met Asp Ala Val Val Ile Ala Val Thr Ser Asp Ser Phe 
                725                 730                 735     


Ile Gln Gln Val Thr Asn Tyr Tyr Lys Arg Lys Glu Arg Arg Glu Leu 
            740                 745                 750         


Asn Gly Asp Asp Lys Phe Pro Leu Pro Trp Lys Phe Phe Arg Glu Glu 
        755                 760                 765             


Val Ile Ala Arg Leu Ser Pro Asn Pro Lys Glu Gln Ile Glu Ala Leu 
    770                 775                 780                 


Pro Asn His Phe Tyr Ser Glu Asp Glu Leu Ala Asp Leu Gln Pro Ile 
785                 790                 795                 800 


Phe Val Ser Arg Met Pro Lys Arg Ser Ile Thr Gly Glu Ala His Gln 
                805                 810                 815     


Ala Gln Phe Arg Arg Val Val Gly Lys Thr Lys Glu Gly Lys Asn Ile 
            820                 825                 830         


Thr Ala Lys Lys Thr Ala Leu Val Asp Ile Ser Tyr Asp Lys Asn Gly 
        835                 840                 845             


Asp Phe Asn Met Tyr Gly Arg Glu Thr Asp Pro Ala Thr Tyr Glu Ala 
    850                 855                 860                 


Ile Lys Glu Arg Tyr Leu Glu Phe Gly Gly Asn Ala Lys Lys Ala Phe 
865                 870                 875                 880 


Ser Thr Asp Leu His Lys Pro Lys Lys Asp Gly Thr Lys Gly Pro Leu 
                885                 890                 895     


Ile Lys Ser Val Arg Ile Met Glu Asn Lys Thr Leu Val His Pro Val 
            900                 905                 910         


Asn Lys Gly Lys Gly Val Val Tyr Asn Ser Ser Ile Val Arg Thr Asp 
        915                 920                 925             


Val Phe Gln Arg Lys Glu Lys Tyr Tyr Leu Leu Pro Val Tyr Val Thr 
    930                 935                 940                 


Asp Val Thr Lys Gly Lys Leu Pro Asn Lys Val Ile Ala Ala Lys Lys 
945                 950                 955                 960 


Gly Tyr Asp Asp Trp Ile Glu Val Asp Asp Ser Phe Thr Phe Leu Tyr 
                965                 970                 975     


Ser Leu Tyr Pro Asn Asp Leu Ile Phe Ile Arg Gln Asn Pro Lys Lys 
            980                 985                 990         


Lys Ile Ser Leu Lys Lys Arg Ile  Glu Ser His Ser Ile  Ser Asp Ser 
        995                 1000                 1005             


Lys Glu  Val Gln Glu Ile Leu  Thr Tyr Tyr Lys Gly  Val Asp Ser 
    1010                 1015                 1020             


Ser Thr  Ala Ala Ile Glu Phe  Ile Thr His Asp Gly  Ser Tyr Tyr 
    1025                 1030                 1035             


Ala Lys  Gly Val Gly Val Gln  Asn Leu Asp Cys Phe  Glu Lys Tyr 
    1040                 1045                 1050             


Gln Val  Asp Ile Leu Gly Asn  Tyr Ser Lys Val Lys  Gly Glu Lys 
    1055                 1060                 1065             


Arg Leu  Glu Leu Glu Thr Ser  Asp Asn Asn His Lys  Gly Lys Asp 
    1070                 1075                 1080             


Val Asn  Ser Ile Lys Ser Thr  Ser Arg 
    1085                 1090         


<210>  631
<211>  1092
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  631

Met Lys Tyr Ser Ile Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Trp 
1               5                   10                  15      


Ser Val Ile Asn Lys Asp Lys Glu Arg Ile Glu Asp Met Gly Val Arg 
            20                  25                  30          


Ile Phe Gln Lys Ala Glu Asn Pro Lys Asp Gly Ser Ser Leu Ala Ser 
        35                  40                  45              


Ser Arg Arg Glu Lys Arg Gly Ser Arg Arg Arg Asn Arg Arg Lys Lys 
    50                  55                  60                  


His Arg Leu Asp Arg Ile Lys Asn Ile Leu Cys Glu Ser Gly Leu Val 
65                  70                  75                  80  


Lys Lys Asn Glu Ile Glu Lys Ile Tyr Lys Asn Ala Tyr Leu Lys Ser 
                85                  90                  95      


Pro Trp Glu Leu Arg Ala Lys Ser Leu Glu Ala Lys Ile Ser Asn Lys 
            100                 105                 110         


Glu Ile Ala Gln Ile Leu Leu His Ile Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Phe Arg Lys Thr Asp Arg Asn Ala Asp Asp Thr Gly Lys Leu Leu 
    130                 135                 140                 


Ser Gly Ile Gln Glu Asn Lys Lys Ile Met Glu Glu Lys Gly Tyr Leu 
145                 150                 155                 160 


Thr Ile Gly Asp Met Val Ala Lys Asp Pro Lys Phe Asn Thr His Val 
                165                 170                 175     


Arg Asn Lys Ala Gly Ser Tyr Leu Phe Ser Phe Ser Arg Lys Leu Leu 
            180                 185                 190         


Glu Asp Glu Val Arg Lys Ile Gln Ala Lys Gln Lys Glu Leu Gly Asn 
        195                 200                 205             


Thr His Phe Thr Asp Asp Val Leu Glu Lys Tyr Ile Glu Val Phe Asn 
    210                 215                 220                 


Ser Gln Arg Asn Phe Asp Glu Gly Pro Ser Lys Pro Ser Pro Tyr Tyr 
225                 230                 235                 240 


Ser Glu Ile Gly Gln Ile Ala Lys Met Ile Gly Asn Cys Thr Phe Glu 
                245                 250                 255     


Ser Ser Glu Lys Arg Thr Ala Lys Asn Thr Trp Ser Gly Glu Arg Phe 
            260                 265                 270         


Val Phe Leu Gln Lys Leu Asn Asn Phe Arg Ile Val Gly Leu Ser Gly 
        275                 280                 285             


Lys Arg Pro Leu Thr Glu Glu Glu Arg Asp Ile Val Glu Lys Glu Val 
    290                 295                 300                 


Tyr Leu Lys Lys Glu Val Arg Tyr Glu Lys Leu Arg Lys Ile Leu Tyr 
305                 310                 315                 320 


Leu Lys Glu Glu Glu Arg Phe Gly Asp Leu Asn Tyr Ser Lys Asp Glu 
                325                 330                 335     


Lys Gln Asp Lys Lys Thr Glu Lys Thr Lys Phe Ile Ser Leu Ile Gly 
            340                 345                 350         


Asn Tyr Thr Ile Lys Lys Leu Asn Leu Ser Glu Lys Leu Lys Ser Glu 
        355                 360                 365             


Ile Glu Glu Asp Lys Ser Lys Leu Asp Lys Ile Ile Glu Ile Leu Thr 
    370                 375                 380                 


Phe Asn Lys Ser Asp Lys Thr Ile Glu Ser Asn Leu Lys Lys Leu Glu 
385                 390                 395                 400 


Leu Ser Arg Glu Asp Ile Glu Ile Leu Leu Ser Glu Glu Phe Ser Gly 
                405                 410                 415     


Thr Leu Asn Leu Ser Leu Lys Ala Ile Lys Lys Ile Leu Pro Tyr Leu 
            420                 425                 430         


Glu Lys Gly Leu Ser Tyr Asn Glu Ala Cys Glu Lys Ala Asp Tyr Asp 
        435                 440                 445             


Tyr Lys Asn Asn Gly Ile Lys Phe Lys Arg Gly Glu Leu Leu Pro Val 
    450                 455                 460                 


Val Asp Lys Asp Leu Ile Ala Asn Pro Val Val Leu Arg Ala Ile Ser 
465                 470                 475                 480 


Gln Thr Arg Lys Val Val Asn Ala Ile Ile Arg Lys Tyr Gly Thr Pro 
                485                 490                 495     


His Thr Ile His Val Glu Val Ala Arg Asp Leu Ala Lys Ser Tyr Asp 
            500                 505                 510         


Asp Arg Gln Thr Ile Ile Lys Glu Asn Lys Lys Arg Glu Leu Glu Asn 
        515                 520                 525             


Glu Lys Thr Lys Lys Phe Ile Ser Glu Glu Phe Gly Ile Lys Asn Val 
    530                 535                 540                 


Lys Gly Lys Leu Leu Leu Lys Tyr Arg Leu Tyr Gln Glu Gln Glu Gly 
545                 550                 555                 560 


Arg Cys Ala Tyr Ser Arg Lys Glu Leu Ser Leu Ser Glu Val Ile Leu 
                565                 570                 575     


Asp Glu Ser Met Thr Asp Ile Asp His Ile Ile Pro Tyr Ser Arg Ser 
            580                 585                 590         


Met Asp Asp Ser Tyr Ser Asn Lys Val Leu Val Leu Ser Gly Glu Asn 
        595                 600                 605             


Arg Lys Lys Ser Asn Leu Leu Pro Lys Glu Tyr Phe Asp Arg Gln Gly 
    610                 615                 620                 


Arg Asp Trp Asp Thr Phe Val Leu Asn Val Lys Ala Met Lys Ile His 
625                 630                 635                 640 


Pro Arg Lys Lys Ser Asn Leu Leu Lys Glu Lys Phe Thr Arg Glu Asp 
                645                 650                 655     


Asn Lys Asp Trp Lys Ser Arg Ala Leu Asn Asp Thr Arg Tyr Ile Ser 
            660                 665                 670         


Arg Phe Val Ala Asn Tyr Leu Glu Asn Ala Leu Glu Tyr Arg Asp Asp 
        675                 680                 685             


Ser Pro Lys Lys Arg Val Phe Met Ile Pro Gly Gln Leu Thr Ala Gln 
    690                 695                 700                 


Leu Arg Ala Arg Trp Arg Leu Asn Lys Val Arg Glu Asn Gly Asp Leu 
705                 710                 715                 720 


His His Ala Leu Asp Ala Ala Val Val Ala Val Thr Asp Gln Lys Ala 
                725                 730                 735     


Ile Asn Asn Ile Ser Asn Ile Ser Arg Tyr Lys Glu Leu Lys Asn Cys 
            740                 745                 750         


Lys Asp Val Ile Pro Ser Ile Glu Tyr His Ala Asp Glu Glu Thr Gly 
        755                 760                 765             


Glu Val Tyr Phe Glu Glu Val Lys Asp Thr Arg Phe Pro Met Pro Trp 
    770                 775                 780                 


Ser Gly Phe Asp Leu Glu Leu Gln Lys Arg Leu Glu Ser Glu Asn Pro 
785                 790                 795                 800 


Arg Glu Glu Phe Tyr Asn Leu Leu Ser Asp Lys Arg Tyr Leu Gly Trp 
                805                 810                 815     


Phe Asn Tyr Glu Glu Gly Phe Ile Glu Lys Leu Arg Pro Val Phe Val 
            820                 825                 830         


Ser Arg Met Pro Asn Arg Gly Val Lys Gly Gln Ala His Gln Glu Thr 
        835                 840                 845             


Ile Arg Ser Ser Lys Lys Ile Ser Asn Gln Ile Ala Val Ser Lys Lys 
    850                 855                 860                 


Pro Leu Asn Ser Ile Lys Leu Lys Asp Leu Glu Lys Met Gln Gly Arg 
865                 870                 875                 880 


Asp Thr Asp Arg Lys Leu Tyr Glu Ala Leu Lys Asn Arg Leu Glu Glu 
                885                 890                 895     


Tyr Asp Asp Lys Pro Glu Lys Ala Phe Ala Glu Pro Phe Tyr Lys Pro 
            900                 905                 910         


Thr Asn Ser Gly Lys Arg Gly Pro Leu Val Arg Gly Ile Lys Val Glu 
        915                 920                 925             


Glu Lys Gln Asn Val Gly Val Tyr Val Asn Gly Gly Gln Ala Ser Asn 
    930                 935                 940                 


Gly Ser Met Val Arg Ile Asp Val Phe Arg Lys Asn Gly Lys Phe Tyr 
945                 950                 955                 960 


Thr Val Pro Ile Tyr Val His Gln Thr Leu Leu Lys Glu Leu Pro Asn 
                965                 970                 975     


Arg Ala Ile Asn Gly Lys Pro Tyr Lys Asp Trp Asp Leu Ile Asp Gly 
            980                 985                 990         


Ser Phe Glu Phe Leu Tyr Ser Phe  Tyr Pro Asn Asp Leu  Ile Glu Ile 
        995                 1000                 1005             


Glu Phe  Gly Lys Ser Lys Ser  Ile Lys Asn Asp Asn  Lys Leu Thr 
    1010                 1015                 1020             


Lys Thr  Glu Ile Pro Glu Val  Asn Leu Ser Glu Val  Leu Gly Tyr 
    1025                 1030                 1035             


Tyr Arg  Gly Met Asp Thr Ser  Thr Gly Ala Ala Thr  Ile Asp Thr 
    1040                 1045                 1050             


Gln Asp  Gly Lys Ile Gln Met  Arg Ile Gly Ile Lys  Thr Val Lys 
    1055                 1060                 1065             


Asn Ile  Lys Lys Tyr Gln Val  Asp Val Leu Gly Asn  Val Tyr Lys 
    1070                 1075                 1080             


Val Lys  Arg Glu Lys Arg Gln  Thr Phe 
    1085                 1090         


<210>  632
<211>  1094
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  632

Met Lys Ser Thr Leu Ala Ile Ala Ser Ile Ser Ser Leu Gly Trp Val 
1               5                   10                  15      


Ile Glu Leu Gly Glu Asn Arg Ile Met Arg Leu Gly Leu Asp Ile Gly 
            20                  25                  30          


Ser Thr Ser Ile Gly Trp Trp Ile Tyr Glu Thr Thr Asp Gly Gln Ile 
        35                  40                  45              


Thr Ser Val Ile Asp Gly Gly Val Arg Ile Phe Ser Asp Gly Arg Asp 
    50                  55                  60                  


Pro Lys Ser Lys Gly Ser Leu Ala Val Asp Arg Arg Val Ala Arg Ala 
65                  70                  75                  80  


Gln Arg Arg Arg Arg Asp Arg Tyr Leu Arg Arg Lys Ala Ala Leu Met 
                85                  90                  95      


Lys Arg Met Ala Glu Val Gly Leu Met Pro Ser Asp Pro Lys Gln Ala 
            100                 105                 110         


Lys Gln Leu Glu Ala Leu Asp Pro Tyr Glu Leu Arg Ala Arg Gly Leu 
        115                 120                 125             


Asp Glu Thr Leu Glu Leu Thr Glu Leu Gly Arg Ala Leu Phe His Leu 
    130                 135                 140                 


Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Ala Gly Asp 
145                 150                 155                 160 


Asn Glu Ser Gly Lys Ile Ile Asp Ala Thr Ala Arg Leu Asp Trp Ala 
                165                 170                 175     


Met Arg Asp His Arg Ala Arg Thr Tyr Gly Glu Phe Leu His Met Arg 
            180                 185                 190         


Arg Gln Lys Ala Asp Thr Pro Arg Thr Thr Pro Ala Val Arg Thr Arg 
        195                 200                 205             


Leu Thr Ile Ala Ala Arg Asp Gly Ala Glu Lys Glu Glu Ser Gly Tyr 
    210                 215                 220                 


Asp Phe Tyr Pro Asp Arg Gln His Leu Arg Asp Glu Phe Asp Ala Leu 
225                 230                 235                 240 


Trp Glu Ala Gln Ala Glu Tyr His Gly Val Leu Thr Glu Asp Leu Arg 
                245                 250                 255     


Asp Glu Ile Gly Thr Ile Ile Phe His Gln Arg Pro Leu Lys Thr Pro 
            260                 265                 270         


Lys Val Gly Lys Cys Leu Phe Thr Asn Glu Glu Arg Val Ala Ser Ala 
        275                 280                 285             


His Pro Leu Asn Gln Arg Arg Ile Leu Val Glu Thr Val Asn Ala Leu 
    290                 295                 300                 


Arg Val Ala Gln Leu Gly Glu Val Ala Arg Ala Leu Thr Leu Glu Glu 
305                 310                 315                 320 


Arg Asp Thr Ile Leu His Ala Leu Asp Asn Lys Ala His Thr Lys Ser 
                325                 330                 335     


Leu Ser Gly Met Ser Ile Lys Leu Lys Ala Leu Gly Lys Val Ile Lys 
            340                 345                 350         


Leu Arg Ser Gly Gln Tyr Phe Thr Leu Glu Thr Ala Asn Arg Asp Ala 
        355                 360                 365             


Ile Ala Cys Asp Pro Val Arg Ala Ser Leu Ser Lys Pro Asp Arg Ile 
    370                 375                 380                 


Gly Ser Tyr Trp Ser Thr Leu Asp His Asn Ala Gln Trp Glu Val Val 
385                 390                 395                 400 


Asn Arg Ile Arg Ala Val Gln Ser Asp Ala Asp His Arg Glu Leu Val 
                405                 410                 415     


Ala Trp Leu Val Ser Gln His Arg Leu Ala Ala Glu His Ala Glu Gly 
            420                 425                 430         


Ala Ala Tyr Ala Pro Leu Pro Asp Gly Tyr Ser Arg Leu Gly Ile Thr 
        435                 440                 445             


Ala Thr Lys Arg Ile Leu Ala Ala Leu Glu Ala Asn Val Ile Pro Tyr 
    450                 455                 460                 


Ser Lys Ala Val Glu Ser Cys Gly Trp His His Ser Asp Glu Arg Thr 
465                 470                 475                 480 


Gly Glu Val Leu Asp Arg Leu Pro Tyr Tyr Gly Glu Ile Leu Asp Arg 
                485                 490                 495     


His Val Ile Pro Gly Thr Tyr Asp Asp Ser Asp Asp Asp Ile Thr Arg 
            500                 505                 510         


Phe Gly Arg Ile Thr Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu 
        515                 520                 525             


Arg Arg Leu Val Asn Arg Ile Ile Glu Val His Gly Lys Pro Asp Glu 
    530                 535                 540                 


Ile Val Val Glu Leu Ala Arg Asp Leu Lys Leu Ser Gln Asp Gln Lys 
545                 550                 555                 560 


Ala Asp Val Glu Arg Thr Ile Lys Lys Asn Thr Gln Ala Ala Ile Arg 
                565                 570                 575     


Arg Gly Glu Lys Leu Glu Glu Met Gly Val Pro Asn Thr Gly Gly Asn 
            580                 585                 590         


Arg Met Val Leu Arg Leu Trp Glu Asp Leu Asn Asp Asp Asp Val Met 
        595                 600                 605             


Lys Arg Phe Cys Pro Tyr Thr Gly Gln Arg Ile Ser Ala Gly Met Leu 
    610                 615                 620                 


Phe Asp Gly Ser Cys Asp Val Asp His Ile Leu Pro Phe Ser Arg Thr 
625                 630                 635                 640 


Leu Asp Asp Gly Phe Thr Asn Lys Thr Leu Cys Leu Lys Glu Ala Asn 
                645                 650                 655     


Arg Lys Lys Arg Asn Tyr Ala Pro His Glu Ala Ala Glu Lys Gly Leu 
            660                 665                 670         


Leu Asp Arg Asp Ala Met Asp Ser Val Ala Lys Tyr Leu Pro Asp Asn 
        675                 680                 685             


Lys Ala Trp Arg Phe Ala Pro Asp Ala Ile Glu Arg Phe Glu Gly Glu 
    690                 695                 700                 


Arg Asp Phe Thr Ala Arg Ala Leu Lys Asp Thr Gln Tyr Leu Ser Arg 
705                 710                 715                 720 


Leu Ala Arg Thr Tyr Leu Asp Ala Leu Tyr Asp Gly Gly Asp Asp Lys 
                725                 730                 735     


Ser His Val Trp Val Val Pro Gly Arg Leu Thr Glu Met Leu Arg Arg 
            740                 745                 750         


His Trp Gly Leu Asn Gly Leu Glu Ala Leu Ala Asp Ser Asp Glu Lys 
        755                 760                 765             


Thr Val Lys Arg Lys Asn Arg Thr Asp His Arg His His Ala Ile Asp 
    770                 775                 780                 


Ala Ala Val Val Ala Ala Thr Asp Arg Ser Leu Ile Gln Lys Ile Ser 
785                 790                 795                 800 


Lys Ile Ala Ala Ser Asp Glu Gln Ala Gly Ala Glu Asp Thr Leu Lys 
                805                 810                 815     


Ser Thr Pro Pro Pro Trp Asp Ser Phe Arg Asp Asp Ile Ala Ala Gln 
            820                 825                 830         


Leu Gln Asn Ile Thr Val Ser His Arg Ala Asp His Gly Arg Thr Asp 
        835                 840                 845             


Ser Leu Ala Lys Gly Arg Gly Glu Asp Thr Thr Ser Gly Ala Leu His 
    850                 855                 860                 


Glu Ala Thr Ala Leu Gly Ile Ile Asp Glu Thr His Val Thr Val Arg 
865                 870                 875                 880 


Ile Pro Ile Glu Ser Leu Lys Ser Gly His Leu Ile Glu Gly Ser Arg 
                885                 890                 895     


Asn Gly Tyr Ile Arg Asp Pro Tyr Leu Arg Asp Gln Leu Thr Leu Ala 
            900                 905                 910         


Thr Lys Asn Gly Ser Asp Lys Asp Phe Thr Thr Val Leu Gln Lys Phe 
        915                 920                 925             


Phe Asp Arg Ser Lys Arg Tyr Gly Gly Thr Arg Arg Val Arg Ile Ile 
    930                 935                 940                 


Lys Thr Leu Gln Asp Thr Ala Arg Ile Ser Val Pro His Asn Ala Pro 
945                 950                 955                 960 


Ile Lys Ala Tyr Lys Gly Gly Ser Asn His Ala Phe Glu Ile Trp Arg 
                965                 970                 975     


Leu Pro Asn Gly Lys Ile Glu Ser Gln Val Ile Thr Thr Trp Glu His 
            980                 985                 990         


His Asn Leu Thr Glu Pro Lys Arg  Pro His Pro Ala Ala  Lys Arg Ile 
        995                 1000                 1005             


Ile Arg  Leu His Lys Thr Asp  Met Val Ala Ile Glu  Arg Asp Gly 
    1010                 1015                 1020             


Lys Thr  Val Ile Cys Phe Val  Gln Lys Thr Asp Pro  Ser Asn Gly 
    1025                 1030                 1035             


Ala Phe  Leu Val Pro His Phe  Glu Ala Asn Ala Asp  Gly Arg Lys 
    1040                 1045                 1050             


Arg Lys  Asn Glu Pro Val Glu  Trp Tyr Gln Met Ser  Ala Asn Ser 
    1055                 1060                 1065             


Leu Ile  Lys Ala Asp Leu Arg  Arg Val Gly Val Asp  Glu Met Gly 
    1070                 1075                 1080             


Arg Val  Arg Asp Pro Gly Pro  Pro Pro Ile Arg 
    1085                 1090                 


<210>  633
<211>  1094
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  633

Met Lys Tyr Arg Val Gly Phe Asp Leu Gly Thr Asn Ser Leu Gly Trp 
1               5                   10                  15      


Ala Val Val Arg Leu Asn Ala Ser Gly Glu Pro Asp Arg Ile Glu Val 
            20                  25                  30          


Ala Gly Ser Arg Ile Phe Ser Asp Gly Arg Glu Pro Lys Ser Gly Ala 
        35                  40                  45              


Ser Leu Ala Gly Ala Arg Arg Asp Ala Arg Ala Met Arg Arg Arg Arg 
    50                  55                  60                  


Asp Arg Phe Lys Gln Arg Gln Ala Ala Leu Ile Lys Tyr Leu Thr Gln 
65                  70                  75                  80  


Ala Gly Phe Phe Pro Ala Asp Glu Ser Glu Arg Lys Ala Leu Ser Ala 
                85                  90                  95      


Leu Asp Pro Tyr Glu Leu Arg Ala Arg Ala Leu Asp Glu Ala Leu Pro 
            100                 105                 110         


Leu Ala His Leu Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly 
        115                 120                 125             


Phe Lys Ser Asn Arg Lys Thr Asp Arg Gly Lys Asn Asp Asp Thr Gly 
    130                 135                 140                 


Lys Ile Arg Ile Gly Val Ala Arg Leu Tyr Glu Gln Met Arg Asp Ala 
145                 150                 155                 160 


Asp Ala Arg Thr Leu Gly Glu Phe Leu His Lys Arg Arg Ala Ala Ala 
                165                 170                 175     


Ser Asp Pro Asn Ala Ile Pro His Val Arg Thr Arg Ile Val Thr Leu 
            180                 185                 190         


Ala Ala Glu Asp Gly Lys Ala Ser Asp Val Tyr Asp Phe Tyr Pro Asp 
        195                 200                 205             


Arg Gln Thr Leu Glu Glu Glu Phe Glu Ala Ile Trp Glu Ala Gln Thr 
    210                 215                 220                 


Ser His His Pro Glu Ala Leu Thr Glu Glu Arg Arg Ala Gln Phe Tyr 
225                 230                 235                 240 


Glu Ile Ile Phe His Gln Arg Pro Leu Lys Gln Pro Lys Ile Gly Phe 
                245                 250                 255     


Cys Thr Leu Leu Pro Pro Glu Lys Arg Leu Pro Lys Ala His Pro Leu 
            260                 265                 270         


Phe Gln Arg Arg Arg Leu Leu Glu Glu Val Asn Ala Leu Arg Ile Val 
        275                 280                 285             


Leu Pro Gly Lys Glu Thr Arg Ala Leu Glu Lys Ala Glu Arg Asp Ala 
    290                 295                 300                 


Leu Leu Ser Lys Leu Asn Gln Arg Lys Lys Ala Ala Tyr Glu Ser Leu 
305                 310                 315                 320 


Arg Lys Ala Leu Lys Leu Asp Pro Asp Ala Arg Phe Asn Lys Glu Ser 
                325                 330                 335     


Asp Asn Arg Glu Asp Met Ile Gly Asp Glu Ile Ala Ala Glu Met Ala 
            340                 345                 350         


His Lys Ser Arg Phe Gly Lys Val Trp Leu Gly Met Ser Ala Glu Ala 
        355                 360                 365             


Gln Ala Gln Val Ile Glu Arg Leu Arg Gly Leu Glu Ser Asp Ala Asp 
    370                 375                 380                 


Ala Asp Asp Phe Glu Ala Trp Leu Arg Asp Asp His Gly Leu Ala Gln 
385                 390                 395                 400 


Glu Gln Ala Lys Ala Val Thr Ala Ala Asn Leu Pro Glu Gly Tyr Gly 
                405                 410                 415     


Arg Phe Gly Glu Thr Ala Thr Arg Lys Leu Ile Ala Ala Leu Glu Gln 
            420                 425                 430         


Glu Val Ile Val Tyr Ser Glu Ala Val Gln Arg Ala Gly Leu Gly His 
        435                 440                 445             


His Ser Asp Leu Arg Asp Gly Gln Val Trp Thr Asp Lys Lys Gly Asn 
    450                 455                 460                 


Pro Ala Leu Pro Tyr Tyr Gly Ala Val Leu Glu Arg His Ile Val Pro 
465                 470                 475                 480 


Gly Thr Ala Asp Pro Asn Glu Thr Asp Asp Ala Leu Arg Ile Gly Arg 
                485                 490                 495     


Leu Thr Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu Arg Arg Val 
            500                 505                 510         


Val Asn Ala Leu Met Arg Arg Tyr Gly Gln Pro Gln Glu Ile Ala Ile 
        515                 520                 525             


Glu Leu Ala Arg Glu Leu Lys Leu Ser Asp Asp Arg Lys Lys Glu Ile 
    530                 535                 540                 


Asn Thr Arg Asn Arg Glu Asn Arg Lys Ala Ala Glu Lys Arg Ser Gln 
545                 550                 555                 560 


Lys Leu Arg Asn Ile Gly Gln Ala Asp Lys Gly Gly Asn Arg Ala Leu 
                565                 570                 575     


Leu Lys Leu Trp Glu Glu Leu Asn Pro Glu Asn Val Leu Asp Arg Arg 
            580                 585                 590         


Cys Val Tyr Thr Gly Arg Val Ile Ser Ile Asp Met Leu Phe Ser Gly 
        595                 600                 605             


Ala Ala Glu Val Asp His Ile Leu Pro Phe Ser Val Ser Leu Asp Asp 
    610                 615                 620                 


Ser Asn Gly Asn Arg Ile Leu Cys Leu Arg Glu Val Asn Arg Ile Lys 
625                 630                 635                 640 


Arg Gln Arg Thr Pro Trp Glu Ala Arg Asp Asp Leu Arg Ala His Phe 
                645                 650                 655     


Gly Pro Asp Ala Gly Trp Glu Ala Ile Ala Ala Arg Ala Ala Arg Leu 
            660                 665                 670         


Pro Arg Glu Lys Arg Trp Arg Phe Glu Pro Asp Ala Ala Asn Arg Phe 
        675                 680                 685             


Asp Ala Gln Gly Gly Phe Leu Ala Arg His Leu Thr Asp Thr Gln His 
    690                 695                 700                 


Leu Ser Arg Leu Ala Arg Ser Tyr Leu Ser Ala Leu Tyr Pro Glu Thr 
705                 710                 715                 720 


Gly Glu Gly Ser Gly His Val Trp Val Ser Pro Gly Arg Leu Thr Glu 
                725                 730                 735     


Met Leu Arg Arg Ala Trp Ser Leu Asn His His Leu Pro Asp His Asn 
            740                 745                 750         


Ile Ala Gly Ala Ala Asn Gln Pro Lys Asn Arg Lys Asp His Arg His 
        755                 760                 765             


His Ala Ile Asp Ala Ile Val Val Ala Leu Ala Asp Arg Ala Met Leu 
    770                 775                 780                 


Asn Arg Ile Ala Arg Glu Ser Gly Lys Glu Gly His Glu Ala Ala Gly 
785                 790                 795                 800 


Arg Leu Val Ala Ser Ile Pro Glu Pro Trp Glu Gly Phe Ser Asp Glu 
                805                 810                 815     


Val Gly Arg Ala Val Arg Ala Ile Thr Val Ser His Arg Pro Asp His 
            820                 825                 830         


Gly Thr Ala Ser Lys Ala Gly Arg Ala Lys Asp Lys Asp Ala Thr Ala 
        835                 840                 845             


Gly Arg Leu His Asn Glu Thr Ala Tyr Gly Leu Thr Gly Glu Lys Asp 
    850                 855                 860                 


Ala Arg Gly Leu Asp Ile Val Val Thr Arg Lys Pro Leu Ser Ala Phe 
865                 870                 875                 880 


Lys Lys Ser Ala Asp Leu Asp Ala Ile Arg Asp Asp Asp Leu Lys Gln 
                885                 890                 895     


Lys Leu Lys Ala Trp Ala Ala Gly Lys Glu Gly Gly Ala Phe Glu Gln 
            900                 905                 910         


Ala Met Arg Ser Phe Gly Asp Pro Glu Arg Gly Pro Arg Ser His Pro 
        915                 920                 925             


Gly Leu Arg Arg Ile Arg Val Thr Glu Pro Leu Ser Val Ile Pro Ile 
    930                 935                 940                 


Arg Asp Arg Asp Gly Arg Pro Tyr Lys Ala Tyr Lys Gly Asp Ser Asn 
945                 950                 955                 960 


Tyr Arg Phe Asp Ile Trp Glu Leu Lys Thr Gly Lys Trp Lys Asp Glu 
                965                 970                 975     


Val Val Ser Met Phe Glu Ala His Arg Pro Asn Trp Thr Ser Pro Ile 
            980                 985                 990         


Arg Ala Gly Asn Pro Thr Ala Arg  Lys Val Leu Ser Val  Arg Gln Asn 
        995                 1000                 1005             


Asp Val  Ile Ala Ile Glu Arg  Glu Asn Gly Pro Glu  Leu Met Arg 
    1010                 1015                 1020             


Val Val  Lys Phe Ser Ala Gly  Ser Leu Ala Leu Ala  Pro Pro Gln 
    1025                 1030                 1035             


Glu Ala  Gly Ser Leu Lys Ala  Arg Asp Ala Asp Lys  Asn Asp Pro 
    1040                 1045                 1050             


Phe Lys  Tyr Val Tyr Gly Ser  Pro Ser Ser Leu Gln  Arg Trp Lys 
    1055                 1060                 1065             


Ala Arg  Gln Val Arg Ile Asp  Glu Leu Gly Arg Val  Phe Asp Pro 
    1070                 1075                 1080             


Gly Phe  Ala Val Arg Ser Arg  Arg Ser Ser Glu 
    1085                 1090                 


<210>  634
<211>  1096
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  634

Met Lys Asn Ile Phe Ser Phe Asp Ile Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Leu Ala Leu Asp Gly Asn Gly Asp Pro Val Arg Ile Ala Asp 
            20                  25                  30          


Ala Gly Val Arg Ile Phe Ser Asp Gly Arg Asp Pro Lys Ser Lys Ala 
        35                  40                  45              


Ser Leu Ala Glu Ala Arg Arg Met Ala Arg Ala Met Ser Arg Arg Arg 
    50                  55                  60                  


Asp Arg Thr Leu Arg Arg Lys Arg Ala Thr Leu Arg Thr Leu Ala Glu 
65                  70                  75                  80  


Ile Gly Leu Met Pro Asp Thr Ser Ser Ala Arg Gln Ala Gln Leu Arg 
                85                  90                  95      


Glu Thr Ser Asp Pro Lys Ser Gly Ser Gly Gln Leu Thr Arg Asn Pro 
            100                 105                 110         


Tyr Ala Leu Arg Ala Leu Ala Leu Glu Glu Lys Leu Pro Leu Phe His 
        115                 120                 125             


Ile Gly Arg Ala Leu Tyr His Leu Gly Gln Arg Arg Gly Phe Gln Ser 
    130                 135                 140                 


Asn Arg Lys Thr Asp Arg Lys Asp Asn Asp Lys Gly Lys Ile Ala Leu 
145                 150                 155                 160 


Gly Ile Glu Glu Leu His Ala Ala Met Arg Gln Asp Gly Ala Gln Thr 
                165                 170                 175     


Tyr Gly Gln Trp Leu Ala Arg Arg His Ala Leu Gly Gln Ala Val Arg 
            180                 185                 190         


Leu Arg Ser Gly Ala Leu Asp Leu Glu Gly Glu Gly Tyr Ala Phe Tyr 
        195                 200                 205             


Pro Glu Arg Lys Leu Leu Glu Asp Glu Phe His Arg Ile Trp Glu Ala 
    210                 215                 220                 


Gln Ala Arg His Tyr Pro Asp Ile Leu Thr Pro Glu Arg Arg Asp His 
225                 230                 235                 240 


Leu Phe Thr Val Met Phe Phe Gln Arg Pro Leu Lys Lys Pro Lys Val 
                245                 250                 255     


Gly Lys Cys Ser Phe Asn Asn Gln Glu Glu Arg Leu Ser Lys Ala His 
            260                 265                 270         


Pro Leu Phe Gln Glu Phe Arg Leu Tyr Lys Glu Val Asn Glu Leu Glu 
        275                 280                 285             


Ile Val Glu Pro Asp Leu Thr His Lys Lys Leu Thr Leu Asp Gln Arg 
    290                 295                 300                 


Asn Ala Leu Val Lys Lys Leu Gln Val Ser Arg Glu Ala Ser Phe Ala 
305                 310                 315                 320 


Ala Leu Arg Lys Val Leu Lys Leu Arg Pro Asp Gln Arg Leu Asn Lys 
                325                 330                 335     


Glu Ser Glu Ser Arg Thr Lys Leu Lys Gly Asn Glu Val Tyr Ala Val 
            340                 345                 350         


Leu Ser Asp Lys Thr Arg Phe Gly Pro Arg Trp Gly Thr Phe Ser Gln 
        355                 360                 365             


Ala Glu Gln Trp Ala Ile Ile Glu Arg Leu Lys Glu Glu Glu Asp Pro 
    370                 375                 380                 


Gly Arg Leu Phe Ala Trp Leu Arg Ser Ala Phe Gly Ile Glu Gly Glu 
385                 390                 395                 400 


Gln Ala Ala Asp Ile Ala Asn Val His Leu Pro Glu Gly His Gly Arg 
                405                 410                 415     


Leu Gly Lys Thr Ala Leu Glu Ala Met Leu Glu Glu Leu Lys Ala Glu 
            420                 425                 430         


Val Ile Thr Glu Ala Glu Ala Ala Lys Arg Cys Gly Tyr Asp His Ala 
        435                 440                 445             


Arg Leu Gly Gly Gly Ser Asp Glu Gly Glu Pro Glu Leu Pro Pro Tyr 
    450                 455                 460                 


Gln Glu Ile Leu Gln His Arg Ile Pro Pro Gly Thr Asn Asn Pro Asp 
465                 470                 475                 480 


Asp Pro Tyr Asp Ile Glu Lys Gly Arg Phe Thr Asn Pro Thr Val His 
                485                 490                 495     


Ile Ala Leu Asn Gln Leu Arg His Val Met Asn Thr Leu Ile Arg Arg 
            500                 505                 510         


Tyr Gly Lys Pro Ala Gln Ile Ala Val Glu Leu Ala Arg Asp Leu Lys 
        515                 520                 525             


Leu Ser Glu Arg Gln Lys Glu Glu Val Asn Arg Thr Ile Ala Arg Asn 
    530                 535                 540                 


Thr Arg Asp Ala Glu Arg Arg Gly Lys Thr Leu Glu Glu Asn Arg Ile 
545                 550                 555                 560 


Pro Asn Thr Gly Tyr Asn Arg Thr Leu Leu Arg Leu Trp Glu Glu Leu 
                565                 570                 575     


Asn Pro Gly Lys Val Glu Asp Arg Cys Cys Ile Tyr Thr Gly Lys Pro 
            580                 585                 590         


Ile Ser Ile Ser Met Leu Phe Ser Gly Glu Val Asp Ile Asp His Ile 
        595                 600                 605             


Leu Pro Trp Ser Arg Thr Leu Asp Asp Ser His Gly Asn Lys Leu Leu 
    610                 615                 620                 


Cys Leu Arg Glu Ala Asn Arg Val Lys Arg Asn Arg Ala Pro Ala Asp 
625                 630                 635                 640 


Val Thr Glu Trp Arg Asp Asn Tyr Asp Glu Ile Leu Ser Arg Ala Ala 
                645                 650                 655     


Arg Leu Pro Gln Asn Lys Arg Asp Arg Phe Ala Pro Asp Ala Met Glu 
            660                 665                 670         


Arg Phe Glu Lys Ala Gly Gly Phe Leu Ala Arg Gln Leu Thr Asp Thr 
        675                 680                 685             


Gln Tyr Leu Ser Arg Met Ala Leu Glu Tyr Leu Ala Cys Leu Phe Pro 
    690                 695                 700                 


Ala Glu Glu Met Asp Gly Phe Gly Glu Leu Arg Arg His Arg Tyr Ile 
705                 710                 715                 720 


Asn Val Cys Pro Gly Arg Leu Thr Glu Met Leu Arg Arg Asn Trp Gly 
                725                 730                 735     


Leu Asn Ser Ile Leu Ser Asp Ser Gln Leu Gly Glu Thr Ala Gln Glu 
            740                 745                 750         


Lys Asn Arg Lys Asp His Arg His His Ala Ile Asp Ala Ile Val Ile 
        755                 760                 765             


Gly Val Thr Gly Arg Ser Leu Leu Asn Arg Ile Ser Tyr Ala Ala Gly 
    770                 775                 780                 


Arg Leu Glu Ala Gln Gln Leu Glu Thr Leu Val Lys Glu Ala Ala Ala 
785                 790                 795                 800 


Thr Asn Lys Pro Trp Asp Gly Phe Arg Glu Asp Val Ala Lys Val Val 
                805                 810                 815     


Gly Gly Ile Ile Val Ser His Lys Pro Asp His Gly Thr Ala Gly Arg 
            820                 825                 830         


Lys Ala Lys Asp Pro Ala Arg Gln Gln Thr Ala Gly Arg Leu His Asn 
        835                 840                 845             


Asp Thr Ala Tyr Gly Leu Thr Gly Glu Thr Asp Ala Arg Gly Asn Thr 
    850                 855                 860                 


Ile Val Val Arg Arg Lys Pro Phe Leu Ser Leu Thr Ser Lys Asp Ile 
865                 870                 875                 880 


Pro Ser Ile Arg Asp Glu Ala Leu Arg Ala Val Leu Glu Gln His Leu 
                885                 890                 895     


Arg Gly Ile Pro Asp Gly Lys Ala Phe Gln Ala Ala Leu Asp Ala Phe 
            900                 905                 910         


Arg Lys Gln Lys Gly Phe His His Tyr His Gly Ile Arg His Val Arg 
        915                 920                 925             


Leu Thr Glu Pro Leu Ser Val Ile Gly Ile Lys Asp Arg Asp Gly Lys 
    930                 935                 940                 


Val Tyr Lys Gly Tyr Lys Gly Asp Ala Asn Gln Arg Tyr Asp Val Trp 
945                 950                 955                 960 


Glu Thr Leu Asp Gly Lys Trp His Ala Glu Val Val Ser Met Phe Asp 
                965                 970                 975     


Ala His Gln Pro Gly Trp Gln Ser Arg Phe His Ala Glu Asn Pro Thr 
            980                 985                 990         


Ala Arg Arg Val Leu Arg Leu Gln  Gln Asp Asp Met Val  Ala Leu Asn 
        995                 1000                 1005             


Ala Pro  Asp Gly Gly Arg Leu  Leu Val Arg Val Val  Lys Phe Arg 
    1010                 1015                 1020             


Gln Asn  Gly Gln Ile Thr Phe  Ala Gly His Asn Glu  Ser Gly Asn 
    1025                 1030                 1035             


Leu Lys  Ala Arg Asp Ala Ala  Ser Asn Glu Gln Asp  Pro Phe Lys 
    1040                 1045                 1050             


Tyr Ile  Ser Arg Met Ala Ser  Ser Leu Lys Asp Cys  Gly Cys Arg 
    1055                 1060                 1065             


Gln Val  Arg Ile Asp Ala Thr  Gly Gln Val Phe Asp  Pro Gly Pro 
    1070                 1075                 1080             


Gln Asp  Arg Ala Ser Arg Leu  Gln Lys Gln Ser Lys  Arg 
    1085                 1090                 1095     


<210>  635
<211>  1097
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  635

Met Lys Lys Gln Pro Gln Arg Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Ala Leu Ile Leu Val Asp Gly Ala Glu Asn Pro 
            20                  25                  30          


Val Gly Leu Leu Asp Cys Gly Val Arg Thr Phe Glu Arg Ala Glu Val 
        35                  40                  45              


Pro Lys Thr Gly Asp Ser Leu Ala Leu Ala Arg Arg Gln Ala Arg Ser 
    50                  55                  60                  


Val Arg Arg Leu Ile Arg Arg Arg Ala His Arg Leu Leu Arg Leu Arg 
65                  70                  75                  80  


Arg Leu Leu Lys Arg Glu Gly Ile Leu Ser Ala Ala Asp Phe Asn Ser 
                85                  90                  95      


Asp Gly Leu Pro Asn Leu Pro Ile Asp Ala Trp Gln Leu Arg Val Ala 
            100                 105                 110         


Gly Leu Glu Arg Lys Leu Thr Asn Gln Glu Trp Ala Ala Val Leu Leu 
        115                 120                 125             


His Leu Val Lys His Arg Gly Tyr Leu Ser Gln Arg Lys Ser Glu Thr 
    130                 135                 140                 


Gln Thr Glu Asn Lys Glu Leu Gly Arg Leu Leu Ala Gly Val Ala Glu 
145                 150                 155                 160 


Asn Ser Lys Leu Leu Ser Glu Ser Ser Glu Gln Tyr Arg Thr Pro Ala 
                165                 170                 175     


Glu Leu Ala Val Lys Leu Phe Ala Glu Gln Ser Gly His Leu Arg Asn 
            180                 185                 190         


Lys Gly Gly Glu Tyr Thr His Thr Phe Asn Arg Leu Asp Leu Gln Lys 
        195                 200                 205             


Glu Leu His Leu Leu Phe Glu Arg Gln Tyr Ala Leu Gly Asn Pro Phe 
    210                 215                 220                 


Ala Ser Ala Glu Leu Gln Ser Lys Val Asp Glu Leu Leu Met Thr Gln 
225                 230                 235                 240 


Arg Ser Ala Leu Gln Gly Glu Ala Val Leu Lys Met Leu Gly Lys Cys 
                245                 250                 255     


Thr Phe Glu Pro Ser Glu Tyr Lys Ala Ala Lys Asn Thr Tyr Ser Ala 
            260                 265                 270         


Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile Leu Tyr 
        275                 280                 285             


Asn Gly Glu Glu Arg Ala Leu Asp Glu Gly Asp Arg Lys Lys Leu Leu 
    290                 295                 300                 


Asp Glu Pro Tyr Lys Lys Ala Lys Leu Thr Tyr Lys Gln Val Arg Ser 
305                 310                 315                 320 


Ile Leu Asn Leu Pro Glu Ser Ala Val Phe Lys Gly Leu Arg Tyr Gly 
                325                 330                 335     


Lys Asp Ser Asp Gly Leu Lys Ala Glu Asn Gly Thr Leu Met Glu Met 
            340                 345                 350         


Lys Ala Tyr His Lys Ile Arg Thr Ile Leu Asp Lys Ala Gly Leu Lys 
        355                 360                 365             


Gly Glu Trp Glu Asn Arg Ile Arg Asn Pro Glu Ile Leu Asp Lys Ile 
    370                 375                 380                 


Gly Thr Ala Phe Ser Leu Tyr Lys Thr Asp Glu Asp Ile Gln Ala Ala 
385                 390                 395                 400 


Leu Ala Asp Leu Arg Leu Pro Glu Asn Val Leu Asp Ala Leu Leu Ala 
                405                 410                 415     


Gly Leu Asn Phe Asp Lys Phe Ile Gln Leu Ser Leu Lys Ala Leu Arg 
            420                 425                 430         


Asn Ile Leu Pro Leu Met Glu Arg Gly Ile Arg Tyr Asp Glu Ala Cys 
        435                 440                 445             


Lys Glu Val Tyr Gly Asp His Arg Ala Leu Ala Glu Ser Lys Asn Gln 
    450                 455                 460                 


Val Leu Leu Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val 
465                 470                 475                 480 


Leu Arg Thr Leu Thr Gln Ala Arg Lys Val Ile Asn Ala Ile Ile Arg 
                485                 490                 495     


Arg Tyr Gly Ser Pro Gln Arg Val His Ile Glu Thr Ala Arg Glu Leu 
            500                 505                 510         


Gly Lys Ser Lys Gln Glu Arg Asp Lys Ile Asp Arg Lys Asn Lys Glu 
        515                 520                 525             


Asn Gln Asn Asp Arg Asn Arg Leu Ala Ser Asp Ile Arg Lys His Phe 
    530                 535                 540                 


Asn Phe Glu Pro Thr Pro Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Asp Leu Arg Glu Glu Lys Gly 
                565                 570                 575     


Glu Cys Leu Tyr Thr Gly Asn Val Ile Glu Leu His Arg Leu Phe Glu 
            580                 585                 590         


Asp Gly Tyr Val Glu Ile Asp His Ala Leu Pro Leu Ser Arg Thr Trp 
        595                 600                 605             


Asp Asn Ser Leu Asn Asn Lys Val Leu Val Leu Lys Ser Ser Asn Gln 
    610                 615                 620                 


Asn Lys Gly Asn Lys Thr Pro Tyr Glu Trp Leu Asp Gly Ala Asn Asn 
625                 630                 635                 640 


Ser Arg Arg Trp Arg Glu Phe Gln Ala Arg Val Gln Gly Cys His Phe 
                645                 650                 655     


Pro Tyr Val Lys Lys Gln Arg Ile Gln Thr Ala Lys Leu Asp Ser Lys 
            660                 665                 670         


Ala Glu Gln Gly Phe Leu Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ile 
        675                 680                 685             


Ser Lys Phe Met Cys Gln Phe Ile Thr Ala Asn Leu Arg Leu Ala Glu 
    690                 695                 700                 


Thr Ser Asn Ala Leu Met Glu Glu Val Ala Gly Glu Asn Pro Tyr Trp 
705                 710                 715                 720 


Lys Glu Arg Lys Arg Gln Arg Val Phe Ala Pro Ser Gly Ser Ile Ile 
                725                 730                 735     


Ala Leu Leu Arg Gly Leu Trp Gly Leu Lys Lys Val Arg Glu Asp Asn 
            740                 745                 750         


Asp Arg His His Ala Leu Asp Ala Ile Val Val Ala Cys Ser Thr Val 
        755                 760                 765             


Ser Met Gln Gln Lys Ile Thr Lys Ala Met Gln Arg Arg Glu Thr Leu 
    770                 775                 780                 


Glu Thr Val Asp Ser Glu Thr Gly Glu Val Lys Thr Arg Ile Pro Gln 
785                 790                 795                 800 


Pro Trp Asp Phe Phe Arg Gln Glu Val Met Ile Arg Val Phe Ser Asp 
                805                 810                 815     


Ser Pro Arg Glu Glu Leu Ala Glu Lys Leu Asn Ser Arg Pro Glu Ala 
            820                 825                 830         


Leu His Asp Phe Val Ser Pro Leu Phe Val Ser Arg Ala Pro Asn Arg 
        835                 840                 845             


Lys Met Thr Gly Gln Gly His Leu Glu Thr Val Lys Ser Ala Lys Arg 
    850                 855                 860                 


Leu Ser Glu Asn Ile Ser Val Val Arg Lys Pro Leu Thr Ala Leu Lys 
865                 870                 875                 880 


Leu Lys Asp Ile Glu Asn Ile Val Gly Tyr Pro Asp Arg Glu Pro Ala 
                885                 890                 895     


Leu Tyr Gln Ala Leu Arg Glu Arg Leu Leu Ala His Lys Asp Asp Pro 
            900                 905                 910         


Val Lys Ala Phe Ala Asp Lys Pro Phe Tyr Lys Pro Ala Lys Asp Gly 
        915                 920                 925             


Gly Gln Gly Ser Leu Val Lys Ala Val Arg Ile Ile Glu Asn Thr Lys 
    930                 935                 940                 


His Gly Leu Lys Ile Arg Asn Gly Ile Thr Asp Thr Asn Gly Asp Met 
945                 950                 955                 960 


Val Arg Thr Asp Val Tyr Gln Lys Thr Asp Lys Lys Gly Lys Arg Gln 
                965                 970                 975     


Tyr Phe Ile Val Pro Val Tyr Ala Trp Gln Val Glu Lys Asn Glu Leu 
            980                 985                 990         


Pro Thr Val Asp Cys Lys Gly Tyr  Glu Ile Asp Asp Asn  Tyr Glu Phe 
        995                 1000                 1005             


Cys Phe  Ser Leu Tyr Lys Tyr  Asp Leu Ile Glu Ile  Gln Asn Pro 
    1010                 1015                 1020             


Asn Asp  Ile Asn Gln Glu Leu  Arg Ala Tyr Tyr Ile  Asn Cys Asp 
    1025                 1030                 1035             


Ser Ser  Asp Gly Arg Phe Trp  Leu Ala Tyr His Asp  Lys Ser Ala 
    1040                 1045                 1050             


Lys Glu  Gln Gln Phe Arg Ile  Ser Thr Gln Asn Gln  Pro Ile Ile 
    1055                 1060                 1065             


Lys Lys  Tyr Gln Val Asp Thr  Leu Gly Lys Glu Ile  Arg Leu Cys 
    1070                 1075                 1080             


Lys Ala  Glu Lys Arg Thr Ala  Leu Asn Gly Lys Lys  Lys Lys 
    1085                 1090                 1095         


<210>  636
<211>  1097
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  636

Met Gly Lys Gly Asp Ile Leu Phe Ser Phe Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Ile Gly Trp Cys Val Phe Ala Leu Ser Asp Asn Gly Asp Pro Cys Arg 
            20                  25                  30          


Ile Leu Asp Ala Asn Ala Arg Ile Phe Ala Asp Gly Arg Glu Pro Lys 
        35                  40                  45              


Ser Gly Asn Ser Leu Ala Glu Gly Arg Arg Ala Ala Arg Ala Met Ala 
    50                  55                  60                  


Arg Arg Arg Asp Arg Tyr Lys Arg Arg Arg Lys Ala Val Leu Arg Thr 
65                  70                  75                  80  


Leu Thr Glu Tyr Gly Leu Met Pro Ala Asp Pro Lys Ala Arg Gln Ala 
                85                  90                  95      


Leu Val Ala Lys Thr Gly Asp Arg Gly Pro Lys Ser Glu Glu Ala Leu 
            100                 105                 110         


Ala Asp Val Tyr Ser Leu Arg Ala Arg Ala Leu Met Glu Lys Leu Pro 
        115                 120                 125             


Leu His His Ile Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly 
    130                 135                 140                 


Phe Lys Ser Asn Arg Lys Thr Asp Arg Lys Ser Asn Glu Lys Gly Ala 
145                 150                 155                 160 


Ile Glu Thr Gly Val Asn Arg Leu Leu Glu Glu Met Arg Asp Thr Gly 
                165                 170                 175     


Ala Pro Thr Leu Gly Ala Tyr Leu Ala Lys Arg Arg Ser Asp Gly Arg 
            180                 185                 190         


Pro Val Arg Val Arg Ala Gly Ala Ser Glu Gln Glu Ala Glu Asp Gly 
        195                 200                 205             


Lys Pro Ala Lys Thr Ala Tyr Asp Phe Tyr Pro Val Arg Arg Leu Leu 
    210                 215                 220                 


Glu Glu Glu Phe Asp Ala Ile Trp Glu Lys Gln Tyr Ser Tyr His Pro 
225                 230                 235                 240 


Glu Val Leu Thr Glu Glu Arg Arg Gly His Leu Phe Arg Val Met Phe 
                245                 250                 255     


His Gln Arg Pro Leu Lys Pro Ala Ile Val Gly Arg Cys Ser Phe Asn 
            260                 265                 270         


Ser Ala Glu Thr Arg Leu Ala Lys Ala His Pro Leu Phe Gln Glu Phe 
        275                 280                 285             


Arg Leu Tyr Lys Glu Val Asn Glu Leu Glu Val Val Met Pro Asp Gln 
    290                 295                 300                 


Ser His Arg Lys Leu Thr Leu Pro Glu Arg Asp Arg Leu Val Leu Lys 
305                 310                 315                 320 


Leu Arg Gly Gln Arg Lys Ala Thr Tyr Ala Ala Leu Arg Lys Glu Leu 
                325                 330                 335     


Arg Leu Arg Asp Leu Arg Phe Asn Lys Glu Thr Glu Asn Arg Thr Asp 
            340                 345                 350         


Met Met Gly Asp Glu Val Thr Ala Ala Leu Ala Asp Lys Ala Val Phe 
        355                 360                 365             


Gly Asn Arg Trp Ser Gly Phe Ser Thr Asp Glu Lys Trp Arg Ile Val 
    370                 375                 380                 


Glu Arg Leu Arg Glu Glu Glu Asp Pro Phe Ala Leu His Glu Trp Leu 
385                 390                 395                 400 


Val Ser Glu His Gly Leu Ser Asp Ala Glu Ala Glu Gln Ala Ala Lys 
                405                 410                 415     


Val His Leu Pro Asp Gly Tyr Gly Arg Leu Gly Pro Thr Ala Val Ser 
            420                 425                 430         


Thr Met Leu Glu Glu Leu Lys Ala Asp Val Ile Thr Glu Ala Asp Ala 
        435                 440                 445             


Ala Arg Leu Ala Gly Tyr Asp His Ser Leu Ala Arg Ala Asp Glu Asp 
    450                 455                 460                 


Gly Leu Asp Arg Leu Pro Lys Tyr Gln Glu Val Leu Glu Arg Arg Ile 
465                 470                 475                 480 


Pro Pro Gly Thr Asn Asp Pro Asp Asp Pro Tyr Asp Ile Ala Lys Gly 
                485                 490                 495     


Arg Ile Thr Asn Pro Thr Val His Ile Ala Leu Asn Gln Phe Arg His 
            500                 505                 510         


Val Val Asn Ala Leu Ala Arg Arg Phe Gly Lys Pro Asp Gln Ile Ala 
        515                 520                 525             


Ile Glu Leu Ala Arg Glu Leu Lys Leu Asn Asp Arg Gln Lys Asp Glu 
    530                 535                 540                 


Val Asn Arg Val Ile Gly Arg Asn Thr Arg Ala Ala Ala Leu Arg Ser 
545                 550                 555                 560 


Gln Lys Leu Arg Asp Ile Gly Gln Lys Asp Asn Gly Tyr Asn Arg Ala 
                565                 570                 575     


Leu Leu Lys Leu Trp Glu Glu Leu Asn Pro Asp Lys Pro Glu Asp Arg 
            580                 585                 590         


Val Cys Val Tyr Thr Gly Arg Pro Ile Cys Ile Ser Met Leu Phe Ser 
        595                 600                 605             


Gly Glu Val Asp Val Asp His Ile Leu Pro Trp Ser Arg Thr Met Asp 
    610                 615                 620                 


Asp Ser Gln Ala Asn Arg Ile Leu Cys Val Val Glu Ala Asn Arg Val 
625                 630                 635                 640 


Lys Ala Asn Arg Ala Pro Ala Glu Val Ala Glu Trp Gly Gly Arg Tyr 
                645                 650                 655     


Asp Glu Ile Leu Glu Arg Ala Ala Arg Leu Pro Arg Asn Lys Arg Trp 
            660                 665                 670         


Arg Phe Ala Arg Asp Ala Met Glu Lys Tyr Lys Glu Asn Glu Gly Phe 
        675                 680                 685             


Leu Ala Arg Gln Leu Thr Asp Thr Gln Tyr Leu Ser Arg Leu Ala Arg 
    690                 695                 700                 


Glu Tyr Ala Glu Ala Leu Tyr Pro Thr Glu Glu Pro Asp Arg Leu Gly 
705                 710                 715                 720 


Glu Leu Arg Arg Arg Asn His Val Arg Val Ile Pro Gly Arg Leu Thr 
                725                 730                 735     


Glu Met Leu Arg Arg Gly Trp Gly Leu Asn Ala Leu Leu Pro Asp His 
            740                 745                 750         


Asn Phe Gly Ala Gly Gln Ala Lys Asn Arg Lys Asp His Arg His His 
        755                 760                 765             


Ala Ile Asp Ala Ala Val Val Gly Val Thr Thr Arg Gly Leu Leu Gln 
    770                 775                 780                 


Arg Ile Ser Thr Glu Ala Gly Leu Arg Glu Lys Leu Ala Leu Glu Glu 
785                 790                 795                 800 


Thr Ile Ala Asp Ile Pro Met Pro Trp Pro Gly Phe Arg Glu Glu Leu 
                805                 810                 815     


Lys Ala Ala Ile Asp Arg Ala Val Val Ser His Lys Pro Asp His Gly 
            820                 825                 830         


Thr Leu Pro Lys Pro Gly Glu His Gly Arg Thr Ala Gly Gln Leu His 
        835                 840                 845             


Asn Asp Thr Ala Tyr Gly Ile Thr Gly Glu Thr Asp Ala Arg Gly Asn 
    850                 855                 860                 


Pro Ile Val Val Arg Arg Lys Pro Phe Leu Ser Leu Thr Asp Lys Asp 
865                 870                 875                 880 


Ile Pro Ser Ile Arg Asp Thr Glu Leu Arg Gly Arg Val Tyr Ser Ala 
                885                 890                 895     


Thr Ser Gly Leu Ser Gly Arg Glu Leu Ser Ala Ala Leu Asp Tyr Phe 
            900                 905                 910         


Arg Lys Thr Asp Lys Gln Phe Arg Gly Ile Arg His Val Arg Val Thr 
        915                 920                 925             


Glu Ser Leu Ser Val Ile Pro Ile Arg Asp Arg Asp Gly Asn Ile Tyr 
    930                 935                 940                 


Lys Gly Tyr Lys Gly Asp Ala Asn Phe Arg Tyr Asp Val Trp Glu Leu 
945                 950                 955                 960 


Pro Asp Gly Lys Trp Lys Ala Asp Val Val Thr Met Phe Asp Ala His 
                965                 970                 975     


Arg Pro Asp Arg Ala Ala Glu Glu Arg Arg Pro His Pro Ala Ala Arg 
            980                 985                 990         


Lys Val Leu Ser Leu Lys Gln Asn  Asp Met Val Ala Cys  Glu His Pro 
        995                 1000                 1005             


Lys Asp  Gly Tyr Thr Ile Gly  Arg Val Val Lys Phe  Asn Thr Ala 
    1010                 1015                 1020             


Gly Gln  Ile Tyr Phe Ala Ala  His Asn Glu Gly Gly  Ser Leu Lys 
    1025                 1030                 1035             


Ala Arg  Asp Ala Asp Arg Ala  Asp Pro Phe Lys Tyr  Phe Ser Lys 
    1040                 1045                 1050             


Ser Ala  Ser Gly Leu Lys Glu  Ile Ala Ala Arg Gln  Val Arg Ile 
    1055                 1060                 1065             


Asp Ala  Thr Gly Gln Val Leu  Asp Pro Gly Pro Gln  Asp Arg Glu 
    1070                 1075                 1080             


Ser Arg  Leu Ala Arg Asn Lys  Arg Leu Glu Arg Glu  Arg Ala 
    1085                 1090                 1095         


<210>  637
<211>  1100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  637

Met Lys Tyr Thr Ile Gly Leu Asp Ile Gly Thr Ser Ser Val Gly Trp 
1               5                   10                  15      


Gly Val Ile Asp Thr Glu Arg Asn Arg Ile Gln Asp Leu Gly Val Arg 
            20                  25                  30          


Leu Phe Glu Ser Ala Glu Asn Gln Lys Asp Gly Glu Ser Leu Ala Lys 
        35                  40                  45              


Pro Arg Arg Asp Ala Arg Ser Met Arg Arg Arg Leu Ala Arg Arg Gly 
    50                  55                  60                  


Ser Arg Leu Glu Arg Val Lys Ser Ile Phe Glu Lys Ala Lys Phe Leu 
65                  70                  75                  80  


Thr Arg Gly Gln Met Gly Ile Thr His Ala Arg Pro Asn Asp Pro Tyr 
                85                  90                  95      


Arg Leu Arg Ala Glu Gly Leu Asp Arg Leu Leu Ser Asn Glu Asp Leu 
            100                 105                 110         


Phe Ile Ala Val Tyr His Leu Ala Lys Arg Arg Gly Tyr Lys Ser Asn 
        115                 120                 125             


Arg Lys Asn Val Leu Glu Gly Asp Gly Glu Asp Val Arg Glu Gly Lys 
    130                 135                 140                 


Ala Val Leu Asp Gly Val Ala Gln Asn Lys Lys Leu Met Ala Phe Arg 
145                 150                 155                 160 


Gly Tyr Arg Thr Val Gly Glu Met Phe Phe Lys Asp Ser Val Phe Thr 
                165                 170                 175     


Glu Ala Lys Arg Asn Lys Ala Gly Ser Tyr Lys His Cys Val Leu Arg 
            180                 185                 190         


Glu Met Leu Glu Asp Glu Leu Gly Lys Ile Leu His Ala Gln Arg Ser 
        195                 200                 205             


Leu Gly Asn Thr Leu Val Thr Asp Ser Phe Ile Ser Lys Leu Leu Glu 
    210                 215                 220                 


Lys Lys Thr Gly Val Phe Val Tyr Gln Arg Pro Phe Ala Ser Gly Asn 
225                 230                 235                 240 


Gln Ile Leu Lys Leu Val Gly Pro Cys Thr Phe Glu Pro Glu Glu Ile 
                245                 250                 255     


Arg Ala Ala Lys Ala Thr Tyr Ser Phe Gln Tyr Phe Asn Val Leu Gln 
            260                 265                 270         


Arg Leu Asn Asn Leu Thr Ile Lys Asn Thr Asp Thr Gly Thr Glu Arg 
        275                 280                 285             


Lys Leu Thr Ser Glu Glu Arg Ala Lys Met Ile Ala Phe Ile Phe Ser 
    290                 295                 300                 


His Lys Asp Val Gln Tyr Ala Gln Ile Arg Leu Glu Leu Lys Leu Ser 
305                 310                 315                 320 


Asp Val Asp Arg Phe Asn Met Val Asn Tyr Phe Val Gln Arg Lys Glu 
                325                 330                 335     


Leu Glu Thr Lys Thr Ala Glu Glu Leu Arg Arg Glu Ile Glu Lys Lys 
            340                 345                 350         


Thr Lys Phe Pro Ser Leu Ser Asp Tyr Tyr Thr Ile Arg Lys Thr Val 
        355                 360                 365             


Ser Glu Ile Asp Ser Ala Phe Trp Ile Gln Ile Glu Lys Gln His Asp 
    370                 375                 380                 


Val Ile Asp Ala Ile Gly Glu Ile Phe Thr Leu Tyr Lys Thr Asp Glu 
385                 390                 395                 400 


Asp Ile Ile Ala Gln Leu Asn Thr Arg Ile Pro Ser Ile Pro Arg Pro 
                405                 410                 415     


Val Val Ser Ala Leu Leu Gly Leu Ser Phe Thr Lys Phe Gly His Leu 
            420                 425                 430         


Ser Leu Lys Ala Leu Arg Lys Ile Ile Pro His Leu Glu Asn Gly Asp 
        435                 440                 445             


Thr Tyr Asp Val Ala Cys Ala Lys Ala Asp Pro Lys Tyr Thr Asp Arg 
    450                 455                 460                 


Ala Lys Thr Arg Ala Arg Lys Leu Arg Pro Leu Asp Lys Glu Asp His 
465                 470                 475                 480 


Ser Ile Thr Asn Pro Val Ala Arg Arg Ala Ile Ala Gln Thr Ile Lys 
                485                 490                 495     


Val Val Asn Ala Ile Ile Asp Lys Tyr Asp Ser Pro Ser Glu Val His 
            500                 505                 510         


Ile Glu Leu Gly Arg Asp Leu Ala Lys Asn Phe Lys Glu Arg Met Lys 
        515                 520                 525             


Ile Gln Lys Glu Gln Lys Gln Asn Gln Glu Gly Asn Glu Arg Ala Ile 
    530                 535                 540                 


Ala Lys Ile His Glu Leu Tyr Lys Leu Glu Asn Pro Arg Gly Gln Asp 
545                 550                 555                 560 


Ile Ile Lys Phe Arg Leu Trp Asn Glu Gln Asn Gly Gln Cys Met Tyr 
                565                 570                 575     


Ser Gly Glu Arg Ile Asp Glu Ile Arg Leu Phe Asp Asn Gly Tyr Thr 
            580                 585                 590         


Glu Ile Asp His Ile Ile Pro Phe Ser Arg Ser Phe Asn Asp Ser Tyr 
        595                 600                 605             


Ser Asn Lys Val Leu Val Leu Lys Met Arg Asn Gln Glu Lys Met Asn 
    610                 615                 620                 


Lys Thr Pro Tyr Glu Tyr Met Gly Gly Asp Ala Asp Thr Trp Gln Arg 
625                 630                 635                 640 


Phe Glu Asn Leu Val Asn Ser Met His Asn Ile Gly Pro Arg Lys Lys 
                645                 650                 655     


Gln Asn Leu Leu Leu Lys Lys Tyr Val Ala Asp Asp Leu Thr Leu Arg 
            660                 665                 670         


Thr Leu Asn Asp Thr Arg Phe Ile Ala Arg Tyr Met Lys Asn Tyr Ile 
        675                 680                 685             


Glu Asn Thr Leu Leu Phe Thr Glu Gly Pro Gln Lys Gln Arg Val Tyr 
    690                 695                 700                 


Thr Val Asn Gly Gln Ala Thr Ala Tyr Leu Arg Lys Arg Trp Val Leu 
705                 710                 715                 720 


His Lys Asp Arg Thr Glu Asn Asn Arg His His Ala Gln Asp Ala Val 
                725                 730                 735     


Val Val Ala Val Ala Thr Pro Ser Leu Val Gln Gly Ile Met Arg His 
            740                 745                 750         


Ala Lys Met Gly Glu Ile Val Asp Tyr Leu His Ser Gln Lys Phe Ala 
        755                 760                 765             


Pro Asn Val Thr Asp Ile Glu Thr Gly Lys Pro Phe Glu Ser Ala Asp 
    770                 775                 780                 


Ile Gln Ser Ala Lys Glu Arg Ile Ile Ala Lys His Glu His Arg Leu 
785                 790                 795                 800 


Arg Ile Pro Glu Pro Trp Glu Gly Phe Ser Asp Glu Leu Asp Ala Arg 
                805                 810                 815     


Met Gly Asp Asp Val Val Glu Lys Leu Ser Leu Arg Lys Ser Asn Ile 
            820                 825                 830         


His Gly Tyr Lys Thr Thr Ser Asp Phe Ser Gln Ile His Pro Ile Phe 
        835                 840                 845             


Val Ser Arg Met Pro Lys Arg Lys Thr Thr Gly Arg Ala His Gln Glu 
    850                 855                 860                 


Thr Leu Arg Ser Pro Arg Arg Phe Lys Glu Phe Gln Glu Ser Val Val 
865                 870                 875                 880 


Arg Thr Pro Leu Ser Ser Ile Asn Leu Lys Lys Leu Glu Glu Met Ser 
                885                 890                 895     


Gly Lys Asn Ser Asp Lys Leu Leu Tyr Asp Ile Leu Arg Lys Arg Leu 
            900                 905                 910         


Leu Ala His Gly Asp Asp Pro Lys Lys Ala Phe Thr Glu Pro Val His 
        915                 920                 925             


Lys Pro Arg Lys Asp Gly Thr Gln Gly Pro Leu Val Arg Ser Ile Lys 
    930                 935                 940                 


Met Val Ser Glu Gly Gln Arg Ser Gly Ile Leu Val Asn Asn Glu Gln 
945                 950                 955                 960 


Ala Leu Val Asp Gln Asp Ser Met Ile Arg Ile Asp Ile Phe Ser Lys 
                965                 970                 975     


Pro Asn Lys Lys Gly Lys Asp Glu Tyr Tyr Ile Ile Pro Val Tyr Ala 
            980                 985                 990         


Tyr His Phe Ala Gln Lys Glu Leu  Pro Asn Lys Val Ile  Thr Ala Lys 
        995                 1000                 1005             


Ala Glu  Ala Asp Trp Arg Thr  Ile Asp Asp Thr Phe  Ala Phe Glu 
    1010                 1015                 1020             


Phe Ser  Leu Tyr Pro Asn Asp  Leu Val Lys Leu Thr  Lys Gly Asp 
    1025                 1030                 1035             


Asp Ile  Arg Phe Gly Tyr Tyr  Ser Gly Cys Asn Arg  Ala Leu Ala 
    1040                 1045                 1050             


Ala Ile  His Ile Leu Pro His  Asp Gln Asn Met Pro  Leu Val Asn 
    1055                 1060                 1065             


Asn Gly  Ile Lys Thr Leu Asp  Lys Ile Asp Lys Tyr  Val Val Asp 
    1070                 1075                 1080             


Ile Leu  Gly Glu Tyr His Lys  Val Gly Lys Glu Thr  Arg Gln Gln 
    1085                 1090                 1095             


Ile Gln  
    1100 


<210>  638
<211>  1100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  638

Met Gly Ala Thr Ile Val Thr Asp Ile Ser Arg Ser Ala Phe Ala Phe 
1               5                   10                  15      


Asp Ile Gly Thr Lys Ser Ile Gly Ser Ala Val Leu Arg Leu Asp Asp 
            20                  25                  30          


Arg Gly Glu Pro Ile Gly Ile Ile Asn Ala Ala Val Arg Ile Tyr Ala 
        35                  40                  45              


Asp Gly Arg Glu Pro Lys Ser Gly Thr Ser Leu Ala Glu Gly Arg Arg 
    50                  55                  60                  


Leu Ala Arg Gly Met Ser Arg Arg Arg Asp Arg Tyr Lys Arg Arg Arg 
65                  70                  75                  80  


Arg Thr Leu Leu Asn Thr Leu Thr Glu Phe Gly Leu Met Pro Pro Thr 
                85                  90                  95      


Asp Glu Ala Arg Lys Ala Leu Leu Ala Glu Thr Asn Asp Arg Arg Phe 
            100                 105                 110         


Gly Ala Val Ala Gly Asn Val Tyr Ala Leu Arg Ala Arg Ala Leu Asp 
        115                 120                 125             


Glu Lys Leu Pro Leu His His Leu Gly Arg Ala Leu Phe His Leu Asn 
    130                 135                 140                 


Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp Lys Arg Asp Asn 
145                 150                 155                 160 


Asp Gln Gly Lys Ile Ala Ser Gly Ile Ser Arg Leu Gly Gln Lys Leu 
                165                 170                 175     


Asp Glu Ala Gly Ala Arg Thr Leu Gly Glu Phe Leu His Arg Gln His 
            180                 185                 190         


Ser Ala Gly Lys Trp Val Arg Val Arg Pro Ser Arg Val Pro Ser Lys 
        195                 200                 205             


Asp Asp Lys Glu Glu Asp Gly Tyr Gly Phe Tyr Pro Glu Arg Ser Phe 
    210                 215                 220                 


Leu Glu Arg Glu Phe Ala Ala Ile Trp Lys Ala Gln Ala Ala Phe Tyr 
225                 230                 235                 240 


Pro Glu Leu Leu Thr Glu Glu Arg Gly Asp His Leu His Arg Val Met 
                245                 250                 255     


Phe Phe Gln Arg Pro Leu Lys Arg Pro Lys Val Gly Lys Cys Ser Phe 
            260                 265                 270         


Tyr Pro Asp Glu Asp Arg Leu Pro Lys Ala His Pro Leu Phe Gln Glu 
        275                 280                 285             


Phe Arg Leu Val Lys Glu Leu Asn Glu Leu Glu Val Val His Arg Gly 
    290                 295                 300                 


Asp Arg Arg Glu Lys Leu Thr Leu Glu Gln Arg Asp Thr Leu Phe Asn 
305                 310                 315                 320 


Gln Leu Arg Thr Gln Arg Ser Ser Ala Phe Thr Ala Leu Arg Lys Thr 
                325                 330                 335     


Leu Lys Ala Pro Thr Gly Thr Arg Phe Asn Lys Glu Ser Asp Ala Arg 
            340                 345                 350         


Asp Lys Leu Lys Gly Asp Glu Val Ala Ala Glu Met Ser His Lys Asp 
        355                 360                 365             


Arg Phe Gly Gln Ala Trp Ile Gly Met Pro Leu Ala Glu Arg Trp Ala 
    370                 375                 380                 


Val Ile Glu Met Leu Leu Asp Thr Glu Asp Pro Leu Glu Val Lys Ala 
385                 390                 395                 400 


Trp Leu Glu Glu His Tyr Gly Phe Ala Ala Asp Arg Ile Asp Ala Ile 
                405                 410                 415     


Ala Ala Ala Lys Leu Pro Pro Gly Tyr Gly Arg Leu Gly Glu Thr Ala 
            420                 425                 430         


Leu Arg Asp Met Leu Asp Glu Leu Lys Asn Gly Thr Val Lys Asp Asp 
        435                 440                 445             


Ser Gly Arg Asp Val Val Leu Thr Glu Arg Gln Ala Met Asp Asn Ile 
    450                 455                 460                 


Trp Lys Asp Asn Pro Ala Leu Gln Arg Val Val Ala Asp Ala Arg Glu 
465                 470                 475                 480 


Leu Pro Pro Tyr Gln Glu Val Leu Gly Arg Arg Ile Pro Pro Gly Thr 
                485                 490                 495     


Gly Lys Pro Glu Asp Pro Tyr Asp Val Gln Lys Gly Arg Leu Thr Asn 
            500                 505                 510         


Pro Thr Val His Ile Gly Leu Asn Gln Leu Lys Arg Val Val Asn Ala 
        515                 520                 525             


Leu Val Arg Arg His Gly Arg Pro Asp Gln Ile Ala Leu Glu Leu Ala 
    530                 535                 540                 


Arg Glu Leu Lys Leu Asn Glu Glu Gln Lys Ala Glu Lys Asp Arg Glu 
545                 550                 555                 560 


Ile Ala Arg Asn Thr Lys Ala Ala Gln Ala Arg Ser Glu Lys Leu Arg 
                565                 570                 575     


Gln Leu Asp Ile Asp Asp Asn Gly Tyr Asn Arg Val Leu Leu Lys Leu 
            580                 585                 590         


Trp Glu Asp Leu Asn Leu Arg Gln Pro Glu Asn Arg Glu Cys Ile Tyr 
        595                 600                 605             


Cys Gly Lys Ala Ile Gly Ile Gly Thr Leu Phe Ser Asn Gln Val Asp 
    610                 615                 620                 


Ile Asp His Ile Leu Pro Trp Ser Lys Thr Leu Asp Asp Ser Gln Ala 
625                 630                 635                 640 


Asn Arg Ile Val Cys His Val Glu Cys Asn Arg Gln Lys Gly Asn His 
                645                 650                 655     


Ala Pro Ala Glu Val Pro Gln Trp His Asp Arg Tyr Asp Asp Ile Leu 
            660                 665                 670         


Ala Arg Ala Glu Arg Leu Pro Arg Asn Lys Ala Trp Arg Phe Gly Arg 
        675                 680                 685             


Glu Ala Met Lys Lys Phe Lys Asp Asp Glu Ser Phe Leu Ala Arg Gln 
    690                 695                 700                 


Leu Thr Asp Thr Gln Tyr Leu Ser Arg Met Ala His Glu Tyr Val Ala 
705                 710                 715                 720 


Ala Leu Tyr Pro Asp Glu Glu Pro Asp Glu Trp Gly Val Phe Thr Arg 
                725                 730                 735     


Arg Asn His Val Arg Val Ile Pro Gly Arg Met Thr Glu Met Leu Arg 
            740                 745                 750         


Arg Gln Trp Gly Leu Asn Ala Ile Ile Ser Asp His Asp Gln Lys Asn 
        755                 760                 765             


Arg Asn Asp His Arg His His Ala Ile Asp Ala Ile Val Val Gly Val 
    770                 775                 780                 


Thr Thr Arg Arg Leu Leu Gln Arg Ile Ala Thr Ala Ala Gly Gly Arg 
785                 790                 795                 800 


Glu Ala Leu Ala Leu Glu Glu Thr Ile Gly Lys Ile Glu Pro Pro Trp 
                805                 810                 815     


Pro Gly Phe Tyr Asp Asp Val Lys Glu Ala Ile Asp Arg Ile Val Val 
            820                 825                 830         


Ser His Lys Pro Asp His Gly Ser Leu Pro Lys Pro Gly Glu Pro Ala 
        835                 840                 845             


Arg Thr Ala Gly Gln Leu His Asn Asp Thr Ala Tyr Gly Phe Thr Gly 
    850                 855                 860                 


Glu Glu Arg Lys Gly Val Pro Ile Met Val His Arg Val Pro Phe Ala 
865                 870                 875                 880 


Thr Leu Thr Glu Lys Asp Ile Pro Ser Ile Arg Asp Arg Gln Leu Ala 
                885                 890                 895     


Glu Ala Leu Tyr Gly Ala Ile Ala Gly Ile Ser Gly Lys Ala Glu Leu 
            900                 905                 910         


Ala Ala Ala Leu Ala Arg Phe Ser Ala Ser Gly Pro Tyr Lys Gly Ile 
        915                 920                 925             


Arg His Val Arg Val Val Glu Pro Val Ser Val Ile Ala Ile Lys Asp 
    930                 935                 940                 


Arg Asp Gly Thr Pro Tyr Lys Gly Tyr Lys Gly Asp Ala Asn Tyr Arg 
945                 950                 955                 960 


Tyr Asp Val Trp Glu Leu Pro Asn Gly Lys Trp Thr His Glu Val Val 
                965                 970                 975     


Ser Met Phe Asp Ala His Arg Pro Asp Trp Gln Ser Glu Ile Arg Asn 
            980                 985                 990         


Lys Asn Pro Ala Ala Arg Lys Val  Leu Ser Leu His Gln  Asn Asp Met 
        995                 1000                 1005             


Val Ala  Tyr Glu His Pro Gln  Asp Gly Tyr Thr Ile  Gly Arg Val 
    1010                 1015                 1020             


Val Lys  Phe Gly Ser Gly Gly  Gln Met Ala Leu Ala  Gly Asn Arg 
    1025                 1030                 1035             


Glu Gly  Gly Ala Leu Lys Ser  Arg Asp Ala Asp Pro  Glu Asp Arg 
    1040                 1045                 1050             


Phe Arg  Tyr Phe Tyr Lys Ser  Gly Gly Ala Met Arg  Asp Ala Arg 
    1055                 1060                 1065             


Leu Arg  Gln Val Arg Val Asp  Glu Thr Gly Arg Val  Phe Asp Pro 
    1070                 1075                 1080             


Gly Pro  Gln Asp Arg Glu Ser  Arg Asn Ala Arg Lys  Gln Ser Ala 
    1085                 1090                 1095             


Ala Gly  
    1100 


<210>  639
<211>  1101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  639

Met Lys Thr Leu Gly Leu Asp Leu Gly Thr Asn Ser Leu Gly Trp Ala 
1               5                   10                  15      


Ile Val Glu Thr Arg Asp Glu Pro Thr Lys Tyr Ser Glu Gly Ser Ile 
            20                  25                  30          


Val Ala Ser Gly Val Arg Ile Phe Ser Gln Ser Glu Met Ala Gly Arg 
        35                  40                  45              


Asp Pro Gln Ser Lys Ala Ser Leu Ala Val Ala Arg Arg Gln Ala Arg 
    50                  55                  60                  


Gly Ser Arg Arg Arg Arg Asp Arg Tyr Leu Lys Arg Arg Gln Arg Leu 
65                  70                  75                  80  


Val Asp Leu Leu Val Asp Leu Gly Leu Met Pro Ala Asp Lys Ala Ala 
                85                  90                  95      


Arg Ala Glu Leu Val Lys Asn His Gly Asp Gly Lys Thr Gly Asp Leu 
            100                 105                 110         


Ser Ser Ser Val Tyr Ala Leu Arg Ala Arg Ala Leu Asp Glu Ala Leu 
        115                 120                 125             


Ser Pro Tyr Glu Ile Gly Arg Ala Ile Phe His Leu Asn Gln Arg Arg 
    130                 135                 140                 


Gly Phe Lys Ser Asn Arg Lys Ala Asp Ser Asn Asp Pro Glu Gln Gly 
145                 150                 155                 160 


Val Ile Ser Glu Gly Met Arg Ala Leu Asp Glu Lys Met Ala Glu Ala 
                165                 170                 175     


Gly Ala Arg Thr Ile Gly Glu Trp Leu His Met Arg Arg Leu Gln Gly 
            180                 185                 190         


Leu Pro Val Arg Ala Arg Ile Ala Pro Asp Gly Glu Gly Tyr Ala Phe 
        195                 200                 205             


Tyr Pro Ser Arg Gly Ala Leu Glu Arg Glu Phe Asp Arg Ile Met Ala 
    210                 215                 220                 


Arg Gln Thr Leu Phe His Thr Asp Leu Leu Thr Asp Thr Ala Ile Arg 
225                 230                 235                 240 


Glu Leu Arg Glu Val Val Phe His Gln Arg Pro Leu Arg Pro Val Lys 
                245                 250                 255     


Pro Gly Lys Cys Ser Tyr Asn Pro Leu Glu Glu Arg Leu Ala Lys Ala 
            260                 265                 270         


His Pro Leu Phe Gln Lys Phe Arg Leu Leu Lys Glu Leu Asn Glu Leu 
        275                 280                 285             


Glu Ile Val Gly Glu Asp Gln Lys His Ile Lys Leu Thr Pro Glu Gln 
    290                 295                 300                 


Arg Asn Ala Leu Ala Leu Gln Leu Arg Thr Ser Leu Thr Lys Gln Gly 
305                 310                 315                 320 


Lys Val Pro Phe Ser Lys Leu Arg Ser Thr Leu Lys Leu Gly Arg Glu 
                325                 330                 335     


Val Arg Phe Asn Lys Glu Thr Asp Asn Arg Ser Asp Leu Glu Gly Asp 
            340                 345                 350         


Val Ile Tyr Phe Arg Met Ser Arg Pro Glu Cys Phe Gly Asn Arg Trp 
        355                 360                 365             


Ala Asp Leu Pro Val Glu Met Gln Ala Ala Ile Thr Glu Arg Leu Arg 
    370                 375                 380                 


Thr Glu Pro Asp Tyr Lys Thr Leu Met Asn Trp Leu Lys Ala Glu Thr 
385                 390                 395                 400 


Gly Leu Asp Glu Val His Ala Arg Ala Val Ala Asp Thr Pro Val Pro 
                405                 410                 415     


Asp Gly Phe Gly Arg Leu Gly Gln Ser Ala Leu Thr Ala Leu Ile Asp 
            420                 425                 430         


Ala Leu Glu Asn Glu Val Gly Glu Gly Gly Phe Val Ile Thr Glu Ala 
        435                 440                 445             


Glu Ala Ala Lys Arg Val Tyr Gly Arg Thr Asn Ser Glu Ala Asp Pro 
    450                 455                 460                 


Asp Arg Lys Gly Val Asp Glu Leu Pro Lys Tyr Gln Glu Val Leu Ala 
465                 470                 475                 480 


Arg His Ile Pro Pro Gly Thr Gly Glu Pro Asp Asp Pro Tyr Asp Asp 
                485                 490                 495     


Phe Met Gly Arg Ile Thr Asn Pro Thr Val His Ile Ala Leu Asn Gln 
            500                 505                 510         


Leu Arg Arg Val Val Asn Ala Leu Ile Arg Thr Tyr Gly Lys Pro Asp 
        515                 520                 525             


Arg Ile Ala Ile Glu Val Gly Arg Asp Leu Lys Met Asn Asp Arg Glu 
    530                 535                 540                 


Arg Asp Glu Val Asn Arg Arg Ile Gly Arg Asn Thr Lys Ala Ala Ile 
545                 550                 555                 560 


Glu Arg Gly Val Lys Leu Thr Glu Thr Phe Arg Gln Leu Asp Thr Gly 
                565                 570                 575     


Tyr Asn Arg Leu Arg Leu Glu Leu Trp Glu Asp Leu Asn Gln Lys Pro 
            580                 585                 590         


Glu Asn Arg Val Cys Ile Tyr Cys Gly Lys Ala Ile Ala Gly His Met 
        595                 600                 605             


Leu Phe Asn Gly Glu Thr Asp Ile Asp His Ile Leu Pro Tyr Ser Lys 
    610                 615                 620                 


Thr Leu Asp Asp Ser Lys Ala Asn Arg Ile Leu Cys Cys Thr Ser Cys 
625                 630                 635                 640 


Asn Arg Glu Lys Lys Asn Arg Pro Pro Ser Arg Val Pro Gln Trp Arg 
                645                 650                 655     


Ser Arg Tyr Gly Glu Ile Leu Ala Arg Ala Thr Thr Leu Pro Lys Asn 
            660                 665                 670         


Lys Gln Trp Arg Phe Ala Glu Asp Ala Met Thr Arg Phe Gly Glu Glu 
        675                 680                 685             


Glu Gly Phe Leu Ala Arg Gln Leu Thr Asp Met Gln Tyr Ile Ser Arg 
    690                 695                 700                 


Leu Ala Leu Thr Tyr Leu Ala His Leu Tyr Asp Tyr Glu Glu Val Asp 
705                 710                 715                 720 


Leu Asp Gly Ile Tyr Lys Arg His Ala Arg Val Arg Ala Leu Pro Gly 
                725                 730                 735     


Arg Met Thr Glu Met Leu Arg Arg Gln Trp Gly Leu Asn Glu Leu Leu 
            740                 745                 750         


His Asp His Asn Leu Ile Gly Gly Asp Val Ala Lys Pro Lys Asn Arg 
        755                 760                 765             


Leu Asp His Arg His His Ala Ile Asp Ala Ile Val Ile Ala Cys Thr 
    770                 775                 780                 


Ser Arg Ala Leu Ile Gln Arg Leu Ala Thr Ala Ser Ala Ala Ala Glu 
785                 790                 795                 800 


Glu Arg Gly Ala Glu Arg Val Val Glu Arg Ile Asp Pro Pro Trp Ser 
                805                 810                 815     


Gly Phe Arg Glu Asp Val Arg Ala Ala Val Gly Ala Ile Val Val Ser 
            820                 825                 830         


His Lys Pro Asp His Gly Thr Val Gly Arg Glu Gly Tyr Ala Lys Gly 
        835                 840                 845             


Arg Gly Gln Thr Ala Gly Lys Leu His Asn Asp Thr Ala Tyr Gly His 
    850                 855                 860                 


Thr Gly Glu Lys Asp Ala Ser Gly Asn Asp Ile Val Val Arg Arg Ile 
865                 870                 875                 880 


Ala Val Thr Asp Ile Arg Ser Pro Ala Asp Ile Arg Lys Ile Arg Thr 
                885                 890                 895     


Asn Ala His Gly His Ser Glu Leu Arg Asp Arg Leu Tyr Glu Ala Thr 
            900                 905                 910         


Arg Gly Leu Glu Gly Lys Ala Phe Glu Gln Ala Val Leu Asp Phe Val 
        915                 920                 925             


Lys Gly Asp Ala Lys Phe Arg Gly Ile Arg His Val Arg Ile Thr Glu 
    930                 935                 940                 


Val Ala Asn Pro Val Trp Ile Thr His Gly Asp Gly Arg His Lys Lys 
945                 950                 955                 960 


Gly Tyr Leu Pro Gly Gly Asn Asp Arg Phe Asp Val Trp Glu Leu Ala 
                965                 970                 975     


Asp Gly Arg Trp Asp Ala Glu Val Val Thr Thr Phe Asp Ala His Arg 
            980                 985                 990         


Pro Gly Phe Val Pro Arg Met Arg  Leu Glu Asn His Asn  Ala Arg Lys 
        995                 1000                 1005             


Ile Met  Ser Leu Lys Lys Gly  Asp Met Ile Ala Tyr  Asn Asp Pro 
    1010                 1015                 1020             


Val Ser  Gly Gln Arg Ile Ile  Ala Ile Val Arg Lys  Phe Asp Gln 
    1025                 1030                 1035             


Arg Asn  Lys Gln Leu Tyr Leu  Asp Pro His Asn Glu  Ala Gly Asn 
    1040                 1045                 1050             


Leu Asp  Arg Arg Glu Lys Glu  Lys Thr Tyr Lys Pro  Leu Arg Pro 
    1055                 1060                 1065             


Met Pro  Asn Pro Leu Lys Lys  Tyr Arg Pro Arg Gln  Val Arg Ile 
    1070                 1075                 1080             


Asp Glu  Ile Gly Gln Val Phe  Asp Pro Gly Pro Trp  Trp Glu Arg 
    1085                 1090                 1095             


Gln Asp  Gly 
    1100     


<210>  640
<211>  1101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  640

Met Glu Gln Thr Ser Leu Asp Leu Ser Gln Ala Met Lys Lys Val Ala 
1               5                   10                  15      


Gln Pro Gln Lys Pro Tyr Arg Leu Gly Val Asp Val Gly Val Ala Ser 
            20                  25                  30          


Ile Gly Ala Ala Ala Val Thr Asp Thr Glu Ile Leu Gly Ile His Val 
        35                  40                  45              


Arg Thr Phe Glu Lys Gly Glu Ile Asn Gly Lys Ser Gly Arg Lys Pro 
    50                  55                  60                  


Leu Asn Ala Asp Tyr Arg Arg Gln Lys Gly Gln Arg Lys Arg Leu Arg 
65                  70                  75                  80  


Arg Lys Lys Asn Arg Leu Leu Ser Leu Arg Arg Leu Phe Arg Arg Val 
                85                  90                  95      


Gly Met Ile Glu Ser Ala Ala Ile Glu Ala Phe Pro Ile Ser Ser Thr 
            100                 105                 110         


Ser Pro Trp Gln Leu Arg Thr Glu Gly Leu Asp Arg Arg Leu Thr Asn 
        115                 120                 125             


Glu Glu Trp Ala Gln Val Leu Tyr His Ile Ala Lys His Arg Gly Phe 
    130                 135                 140                 


Gln Ser Asn Arg Arg Val Asp Met Asp Glu Ala Thr Lys Leu Ser Asp 
145                 150                 155                 160 


Ala Ala Gly Asp Ser Pro Glu Thr Thr Thr Asn Thr Glu Ser Asp Ser 
                165                 170                 175     


Asp Thr Lys Lys Met Leu Ala Gly Val Ala Asp Asn Lys Arg Leu Leu 
            180                 185                 190         


Ala Glu Lys Gly Tyr Arg Thr Ile Gly Glu Ala Val Ala Arg Asp Pro 
        195                 200                 205             


Lys Phe Ser His Arg Ile Arg Asn Lys Glu Glu Ser Tyr Ser His Thr 
    210                 215                 220                 


Ile Gly Arg Asp Glu Ile Asn Ala Glu Leu Asn Cys Leu Phe Gln Ala 
225                 230                 235                 240 


Gln Asn His Tyr Gly Asn Pro His Ala Thr Glu Glu Leu Ser Glu Gln 
                245                 250                 255     


Ile Ile Asp Leu Ile Asn Trp Arg Arg Lys Thr Val Thr Gly Asp Asp 
            260                 265                 270         


Ile Lys Arg Met Val Gly Arg Cys Thr Leu Glu Pro Lys Glu Tyr Arg 
        275                 280                 285             


Ala Ala Ile His Ser Val Ser Ala Glu Glu Phe Arg Trp Leu Ser Thr 
    290                 295                 300                 


Leu Ser Arg Leu Arg Ile Val Thr Gln Gly Glu Ser Arg Pro Leu Thr 
305                 310                 315                 320 


Lys Cys Glu Trp Asp Lys Val Val Asp Leu Pro Trp Arg Gln Glu Ala 
                325                 330                 335     


Ile Thr Tyr Thr Ser Ile Arg Asn Ala Ile Gly Leu Arg His Asp Ile 
            340                 345                 350         


Arg Ser Arg Phe Asn Ile Arg Tyr Glu Thr Gly Gly Lys Lys Thr Lys 
        355                 360                 365             


Thr Asp Thr Lys Asn Glu Thr Thr Lys Gly Gln Ser Ala Asp Ala Val 
    370                 375                 380                 


Thr Ser Glu Val Lys Thr Ala Glu Lys Glu Thr Ala Phe Lys Ala Lys 
385                 390                 395                 400 


Gly Tyr His Ile Leu Arg Lys Ala Tyr Lys Ala Phe Gly Tyr Asp Glu 
                405                 410                 415     


Gln Trp Asp Arg Asp Arg Phe Asn Thr Glu Leu Leu Asp Thr Leu Ala 
            420                 425                 430         


Thr Ala Val Thr Phe Tyr Lys Asp Asp Ser Glu Ile Gln Ala His Leu 
        435                 440                 445             


Glu Thr Asn Asp Thr Pro Glu Tyr Ala Ile Gln Ile Ala Leu Arg Leu 
    450                 455                 460                 


Pro Thr Leu Thr Lys Phe Ser Lys Leu Ser Asn Lys Ala Ile Leu Lys 
465                 470                 475                 480 


Ile Leu Pro His Met Arg Glu Arg Gln Ser Tyr Ser Asn Ala Cys Leu 
                485                 490                 495     


Leu Ala Gly Tyr Lys Asn His His Lys Gln Tyr Asp Asp Thr Pro Gln 
            500                 505                 510         


Asn Gly Lys Ile Pro Arg Ser Ala Ile Glu His Ile Lys Asn Thr Val 
        515                 520                 525             


Val Tyr Arg Ser Leu Asn Gln Ala Arg Arg Val Ile Asn Ala Met Val 
    530                 535                 540                 


Lys Glu Tyr Gly Ala Pro Gln Glu Val His Ile Glu Leu Ala Arg Asp 
545                 550                 555                 560 


Met Glu Lys Ser Ala Lys Lys Lys Asp Glu Ile Asp Leu Trp Met Lys 
                565                 570                 575     


Lys Arg Lys Lys Lys Asn Asp Glu Leu Arg Glu Arg Phe Gly Lys Asn 
            580                 585                 590         


Leu Ser Arg Asp Glu Leu Leu Lys Tyr Lys Leu Tyr Glu Glu Gln Asn 
        595                 600                 605             


Gly Gln Cys Ala Tyr Ser Leu Gln Pro Ile Gln Leu Gly Arg Leu Leu 
    610                 615                 620                 


Glu Pro Asn Tyr Val Glu Val Asp His Ala Leu Pro Arg Ser Arg Ser 
625                 630                 635                 640 


Met Asp Asn Ser Tyr Asn Asn Lys Val Leu Val Leu Thr Lys Gln Asn 
                645                 650                 655     


Arg Asp Lys Gly Asp Arg Thr Pro Phe Glu Tyr Phe Gly Glu Asp Gln 
            660                 665                 670         


Glu Ser Val Asp Trp Lys Arg Phe Thr Asn Trp Val Glu Ser Asn Pro 
        675                 680                 685             


Gln Met Pro Gln Ala Lys Arg Arg Lys Leu Leu Thr Lys Ser Leu Gln 
    690                 695                 700                 


Lys Gly Ile Leu Asp Asn Phe Ser Ala Arg Asn Leu Ser Asp Thr Arg 
705                 710                 715                 720 


Tyr Ile Ala Arg Glu Leu Lys Thr Leu Ile Glu His Tyr Ile Leu Asn 
                725                 730                 735     


Thr Pro Glu Arg Glu Lys Thr Lys Tyr Glu Arg Cys Val Val Ile Ser 
            740                 745                 750         


Gly Lys Leu Thr Ala Gln Met Arg Gln Leu Trp Gly Ile Ser Lys Ala 
        755                 760                 765             


Arg Asp Val Ser Asp Arg His His Gly Ser Asp Ala Ala Val Ile Ala 
    770                 775                 780                 


Cys Ile Thr Pro Ser Met Val Arg Arg Ile Thr Arg Tyr Leu Gln Leu 
785                 790                 795                 800 


Gln Glu Arg Arg Ala Met Pro Ser Ala Pro Gly Lys Gly Asp Glu His 
                805                 810                 815     


Pro Ala Val Val Ala Arg Ala Ile Pro His Ala Pro Val Pro Trp Pro 
            820                 825                 830         


Thr Phe Glu His Asp Leu Glu Ala Ala Leu Glu Lys Val Leu Val Ser 
        835                 840                 845             


Arg Pro Gln Ser Arg Lys Trp Ser Gly Pro Ile His Ala Asp Thr Ile 
    850                 855                 860                 


Tyr Gly Glu His Lys Asp Gln Glu Thr Asn Thr Val Ala Pro Thr Gln 
865                 870                 875                 880 


Arg Ile Lys Leu Thr Gln Leu Thr Lys Ser Asn Tyr Lys Asn Ile Val 
                885                 890                 895     


Gly Tyr Asp Glu Glu Arg Asn Lys Asp Phe Ile Asp Gln Ile Glu Glu 
            900                 905                 910         


Arg Leu Glu Cys His Asn Phe Asn Gly Lys Gln Ala Phe Gly Thr Ser 
        915                 920                 925             


Gln Ser Pro Ile Tyr Arg Ile Lys Lys Asn Gly Glu Leu Gly Pro Glu 
    930                 935                 940                 


Val Lys Lys Val Thr Ile Lys Leu Pro Ile Lys Asn Ser Val Phe Arg 
945                 950                 955                 960 


Ile Arg Arg Gly Tyr Val Lys Asn Ala Lys Asn Ser Thr Ile Tyr Ile 
                965                 970                 975     


Asn Ile Tyr Gln Asn Thr Lys Thr Gly Lys Phe Glu Met His Pro Val 
            980                 985                 990         


Tyr Ala Lys Asp Leu Thr Ser Ser  Ala Asn Ala Ser Lys  Ser Phe Glu 
        995                 1000                 1005             


Tyr Ser  Pro Asp Leu Gln Leu  Val Asp Thr Ile Arg  Arg Gly Asp 
    1010                 1015                 1020             


Tyr Ile  Glu Ile Thr Arg Arg  Asn Gly Glu Val Ile  Ser Gly Tyr 
    1025                 1030                 1035             


Tyr Ala  Thr His Asn Ile Ser  Thr Ser Val Leu Glu  Leu Gln Pro 
    1040                 1045                 1050             


His Asp  Thr Thr Asp Ser Arg  Asn Asn Arg Thr Pro  Ala Ile Lys 
    1055                 1060                 1065             


Thr Ser  Leu Asn Ile Glu Arg  Phe His Ile Asp Ile  Leu Gly Arg 
    1070                 1075                 1080             


Lys Arg  Arg Val Val Arg Arg  Ala Lys Asn Gly Leu  Ala Asp Asp 
    1085                 1090                 1095             


Arg Asp  Lys 
    1100     


<210>  641
<211>  1102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  641

Met Gly Tyr Arg Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Tyr 
1               5                   10                  15      


Ala Ile Leu Lys Thr Asp Glu Lys Gly Ser Pro Tyr Lys Ile Glu His 
            20                  25                  30          


Leu Thr Ser Val Ile Phe Pro Ile Ala Glu Asn Pro Lys Asp Gly Lys 
        35                  40                  45              


Ser Leu Ala Met Pro Arg Arg Glu Gln Arg Gly Ala Arg Arg Arg Asn 
    50                  55                  60                  


Arg Arg Thr Lys Phe Arg Lys His Arg Thr Lys Met Leu Phe Val Arg 
65                  70                  75                  80  


Ser Glu Leu Leu Thr Ile Lys Gln Ile Glu Ala Ile Tyr Ser Glu Lys 
                85                  90                  95      


Gln Lys Leu Ser Ser Ile Tyr Glu Leu Arg Thr Lys Ala Leu Asn Glu 
            100                 105                 110         


Arg Leu Thr Asn Glu Glu Leu Phe Arg Ile Leu Tyr Phe Phe Ser Gly 
        115                 120                 125             


His Arg Gly Phe Lys Ser Asn Arg Lys Ser Glu Leu Thr Glu Lys Asp 
    130                 135                 140                 


Met Gly Pro Val Leu Thr Ala Ile Lys Asp Ile Glu Glu Lys Leu Ala 
145                 150                 155                 160 


Asp Gly Thr Tyr Arg Thr Leu Gly Glu Tyr Met Glu Leu Asp Glu Lys 
                165                 170                 175     


Tyr Gln Glu His Lys Arg Asn Lys Asp Gly Ile Asp Arg Tyr Leu Gly 
            180                 185                 190         


Thr Ala Lys Arg Ser Leu Ile Glu Asp Glu Ile Lys Gln Ile Ile Ile 
        195                 200                 205             


Ala Gln Gln Gln Phe Gly Asn Thr Lys Leu Thr Ser Gly Phe Gln Val 
    210                 215                 220                 


Ala Phe Ile Gly Asp Glu Glu Gln Thr Gly Ile Phe Asn Asp Gln Arg 
225                 230                 235                 240 


Glu Phe Glu Asp Gly Pro Gly Lys Gln Ser Pro Tyr Ala Gly Asn Gln 
                245                 250                 255     


Ile Glu Lys Met Ile Gly Ala Cys Thr Phe Glu Glu Asn Glu Lys Arg 
            260                 265                 270         


Ala Pro Lys Ala Ser Tyr Thr Phe Gln Tyr Phe Asp Phe Leu Cys Lys 
        275                 280                 285             


Leu Asn Asn Leu Lys Tyr Arg Glu Thr Ile Gly Gly Glu Tyr Arg Phe 
    290                 295                 300                 


Leu Ser Leu Glu Gln Lys Asn Val Leu Lys Glu Lys Val Leu Thr Thr 
305                 310                 315                 320 


Gln Lys Ile Thr Phe Ser Gly Val Lys Lys Leu Leu Asp Leu Pro Glu 
                325                 330                 335     


His Ala Gln Phe Asn Leu Val Asn Tyr Gly Thr Lys Ser Thr Val Lys 
            340                 345                 350         


Glu Thr Glu Thr Lys Ser Val Phe Tyr Ala Met Ile Ser Tyr His Lys 
        355                 360                 365             


Leu Lys Lys Ala Ile Pro Ala Pro Ile Phe Ser Asn Leu Ser Thr Ala 
    370                 375                 380                 


Glu Leu Asp Glu Ile Gly Arg Ile Leu Thr Leu Tyr Ser Ser Asp Lys 
385                 390                 395                 400 


Gly Arg Lys Asn Glu Phe Tyr Ser Arg Leu Ala Ile Pro Glu Glu Val 
                405                 410                 415     


Val Glu Ala Leu Leu Pro Ile Asn Phe Ser Lys Phe Gly Asn Leu Ser 
            420                 425                 430         


Leu Lys Ala Met Arg Lys Ile Ile Pro Tyr Leu Glu Met Gly Leu Val 
        435                 440                 445             


Tyr Ser Glu Ala Ala Thr Gln Ala Gly Tyr Asp Phe Arg His Lys Lys 
    450                 455                 460                 


Ile Asp Gln Ala Tyr Ile His Glu Asn Val Thr Asn Pro Val Val Lys 
465                 470                 475                 480 


Arg Ala Ala Ser Gln Thr Ile Lys Ile Val Asn Asn Ile Ile Arg Lys 
                485                 490                 495     


Tyr Gly Glu Pro Asp Gly Ile Asn Ile Glu Leu Ala Arg Glu Leu Ser 
            500                 505                 510         


Lys Asn Phe Ser Glu Arg Lys Lys Thr Val Lys Tyr Gln Glu Glu Asn 
        515                 520                 525             


Arg Ala Arg Asn Glu Arg Ile Ala Asp Lys Leu Arg Glu Cys Glu Leu 
    530                 535                 540                 


Pro Val Asn Gly Ala Asn Ile Thr Arg Leu Lys Leu Phe Glu Glu Gln 
545                 550                 555                 560 


Gly Glu Phe Asp Pro Tyr Thr Gly Leu Lys Ile Pro Phe Asp Arg Ala 
                565                 570                 575     


Phe Ser Glu Glu Tyr Glu Val Asp His Ile Ile Pro Tyr Ser Lys Thr 
            580                 585                 590         


Leu Asp Asp Ser Tyr Gly Asn Lys Ile Leu Val Ser Ala Lys Ala Asn 
        595                 600                 605             


Gln Glu Lys Gly Asn Arg Ile Pro Met Glu Tyr Leu Gln Gly Lys Pro 
    610                 615                 620                 


Glu Arg Ile Lys Lys Leu Glu Ser Ile Thr Ala Thr Ile Lys Asn Pro 
625                 630                 635                 640 


Lys Lys Arg Glu Lys Leu Leu Lys Gln Gln Leu Thr Lys Glu Asp Val 
                645                 650                 655     


Asp Gly Trp Lys Thr Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Ser 
            660                 665                 670         


Leu Leu His Asp Tyr Phe Asn Gln Asn Ile Ser Phe Ala Lys Leu Glu 
        675                 680                 685             


Ser Glu Lys Lys Lys Arg Val Ile Ala Val Asn Gly Ala Ile Thr Ser 
    690                 695                 700                 


Lys Leu Arg Ala Arg Trp Gly Phe Asn Lys Ile Arg Glu Asn Gly Asp 
705                 710                 715                 720 


Glu His His Ala Leu Asp Ala Leu Ile Val Ala Cys Val Thr Asp Lys 
                725                 730                 735     


Tyr Ile Lys Glu Ile Thr Ser Phe Ser Lys Arg Lys Glu Val Arg Phe 
            740                 745                 750         


Asn Glu Asn Leu Trp Lys Arg Glu Lys Thr Pro Gln Glu Ile Glu Gln 
        755                 760                 765             


Glu Val Val His Phe Lys Lys Asp Tyr Asp Arg Ile Phe Asn Gln Ala 
    770                 775                 780                 


Phe Pro Leu Pro Trp Thr Asn Phe Arg Glu Glu Val Ser Cys Arg Met 
785                 790                 795                 800 


Ser Glu Asn Pro Ala Glu Leu Met Lys Asn His Thr Trp Glu Thr Tyr 
                805                 810                 815     


Thr Asp Asp Glu Ile Ala Ala Leu Arg Pro Ile Phe Ile Val Arg Met 
            820                 825                 830         


Pro Asn Met Lys Thr Ser Gly Pro Ala His Gln Glu Thr Ile Arg Ser 
        835                 840                 845             


Gly Lys Leu Glu Glu Lys Gly Leu Asn Ile Ser Arg Val Ser Ile Glu 
    850                 855                 860                 


Lys Leu Lys Leu Asp Lys Glu Gly Thr Ile Lys Ser Gly Asn Ala Gln 
865                 870                 875                 880 


Phe Tyr Lys Asp Ser Gly Asn Gly Trp Gln Leu Leu His Asp Lys Leu 
                885                 890                 895     


Lys Val Ala Leu Glu Lys His Asp Gly Asp Gly Ser Lys Ala Phe Pro 
            900                 905                 910         


Asp Gly Val Phe Lys Tyr Gln Tyr Leu Asp Lys Glu His Thr Val Arg 
        915                 920                 925             


Lys Val Arg Leu Val Ala Lys Ser Thr Leu Gln Thr Ser Leu Asn Glu 
    930                 935                 940                 


Lys Lys Ser Val Ala Asp Asn Gly Ser Met Ile Arg Ile Asp Val Phe 
945                 950                 955                 960 


Arg Asn Glu Gln Gly Lys Tyr Gln Phe Val Pro Ile Tyr Ala Lys Asp 
                965                 970                 975     


Arg Val Ala Lys Lys Leu Pro Asn Gln Ala Val Ala Val Gly Lys Ile 
            980                 985                 990         


Ala Lys Glu Trp Pro Leu Ile Glu  Glu Ser Asp Phe Lys  Phe Ser Leu 
        995                 1000                 1005             


Tyr Ser  Asn Asp Leu Ile Arg  Val Lys His Lys Lys  Gly Val Lys 
    1010                 1015                 1020             


His Val  Ser Lys Asp Gly Asn  Lys Glu Glu Lys Ser  Lys Thr Asp 
    1025                 1030                 1035             


Phe Phe  Gly Tyr Tyr Thr Gly  Ala Asp Ile Ala Thr  Ala Ser Ile 
    1040                 1045                 1050             


Ser Gly  Ile Ser Pro Asn Asn  Phe Phe Glu Phe Arg  Ser Met Gly 
    1055                 1060                 1065             


Leu Ala  Thr Leu Ile Ser Ile  Glu Lys Tyr Lys Val  Asp Tyr Phe 
    1070                 1075                 1080             


Gly Asn  Tyr His Gln Val Asn  Glu Lys Val Arg Gln  Lys Phe Pro 
    1085                 1090                 1095             


Lys Asn  Arg Met 
    1100         


<210>  642
<211>  1103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  642

Met Lys Tyr Asp Ile Gly Ile Asp Ile Gly Val Ala Ser Val Gly Glu 
1               5                   10                  15      


Ala Val Ile Asp Gln Glu Gly Asn Ile Leu Glu Ala Cys Ser Asn Leu 
            20                  25                  30          


Phe Asp Glu Ala Asp Ala Ala Ser Asn Val Asp Arg Arg Asn Phe Arg 
        35                  40                  45              


Glu Gly Arg Arg Asn Lys Arg Arg Glu Arg Thr Arg Val Asn Asp Phe 
    50                  55                  60                  


Lys Lys Leu Trp Thr Lys Phe Gly Phe Glu Ile Pro Lys Asn Val Met 
65                  70                  75                  80  


Asn Asp Thr Ile Leu Leu Arg Asn Lys Gly Ile Lys Cys Glu Leu Asp 
                85                  90                  95      


Leu Thr Glu Leu Tyr Ser Val Leu Leu Tyr Met Leu Lys His Arg Gly 
            100                 105                 110         


Ile Ser Tyr Leu Glu Asp Ala Ile Asp Glu Ala Lys Gly Ser Asn Tyr 
        115                 120                 125             


Ala Lys Gly Ile Ala Leu Asn Gln Lys Glu Leu Lys Glu Lys Leu Pro 
    130                 135                 140                 


Cys Glu Ile Gln Leu Glu Arg Leu Lys Ile Tyr Gly Ser Tyr Arg Gly 
145                 150                 155                 160 


Asp Cys Ile Val Lys Lys Glu Asp Glu Asp Glu Tyr His Ser Asn Val 
                165                 170                 175     


Phe Thr Ile Ser Ala Tyr Lys Lys Glu Leu Glu Ile Leu Phe Arg Asn 
            180                 185                 190         


Gln Lys Leu Pro Glu Glu Phe Ile Gln Gly Tyr Met Lys Ile Phe Glu 
        195                 200                 205             


Arg Lys Arg Glu Tyr Tyr Ile Gly Pro Gly Asn Glu Lys Ser Arg Thr 
    210                 215                 220                 


Asp Tyr Gly Val Tyr Thr Thr Asn Lys Asp Glu Glu Gly His Tyr Ile 
225                 230                 235                 240 


Thr Asp Lys Asn Ile Phe Glu Lys Leu Ile Gly Lys Cys Ser Val Tyr 
                245                 250                 255     


Lys Asp Glu Phe Arg Ala Ala Gly Ala Ser Tyr Thr Ala Gln Glu Phe 
            260                 265                 270         


Asn Val Leu Asn Asp Leu Asn Asn Leu Thr Val Asn Asn Arg Lys Leu 
        275                 280                 285             


Thr Glu Asn Glu Lys Lys Lys Ile Val Glu Lys Ile Lys Ser Ser Asn 
    290                 295                 300                 


Ala Val Asn Met Arg Ser Ile Ile Lys Lys Val Leu Gly Glu Asp Ile 
305                 310                 315                 320 


Glu Gln Phe Gly Gly Ala Arg Lys Asp Lys Ala Asp Lys Glu Ile Tyr 
                325                 330                 335     


His Thr Phe Glu Val Tyr Arg Lys Met Lys Lys Glu Phe Glu Lys Asn 
            340                 345                 350         


Gly Ile Asp Ile Ala Asp Phe Ser Arg Glu Lys Leu Asp Glu Ile Gly 
        355                 360                 365             


Asn Ile Leu Thr Leu Asn Thr Glu Thr Glu Gly Ile Lys Gly Ala Leu 
    370                 375                 380                 


His Glu Ala Lys Asn Ser Phe Thr Tyr Ser Glu Ile Glu Thr Leu Ile 
385                 390                 395                 400 


Asp Ile Arg Lys Lys Asn Ser Ser Leu Phe Ser Lys Trp Gln Ser Leu 
                405                 410                 415     


Ser Leu Lys Ile Met Asn Glu Leu Ile Pro Gln Met Tyr Ala Gln Pro 
            420                 425                 430         


Lys Glu Gln Met Gln Leu Leu Thr Glu Met Gly Val Phe Lys Phe Lys 
        435                 440                 445             


Ser Val Lys Phe Lys Asn Ser Lys Tyr Ile Pro Val Glu Glu Ile Ile 
    450                 455                 460                 


Lys Asp Ile Tyr Asn Pro Val Val Val Arg Ser Val Arg Thr Thr Ile 
465                 470                 475                 480 


Lys Met Ile Asn Glu Thr Ile Arg Lys Tyr Gly Tyr Pro Asp Asn Ile 
                485                 490                 495     


Ile Ile Glu Met Pro Arg Asp Lys Asn Ser Thr Glu Glu Lys Glu Arg 
            500                 505                 510         


Ile Lys Lys Glu Gln Ser Lys Asn Glu Lys Glu Tyr Glu Gly Ile Leu 
        515                 520                 525             


Lys Arg Ile Lys Asp Glu Tyr Asp Ile Glu Ile Asn Arg Asp Asp Tyr 
    530                 535                 540                 


Arg Lys Gln Glu Lys Leu Ala Leu Lys Leu Lys Leu Trp Asn Glu Gln 
545                 550                 555                 560 


Glu Gly Arg Cys Pro Tyr Ser Gly Lys Met Ile Ser Ile Tyr Gly Leu 
                565                 570                 575     


Leu Glu Asp Pro Gln Leu Phe Glu Ile Asp His Ile Ile Pro Lys Ser 
            580                 585                 590         


Ile Ser Phe Asp Asp Ser Arg Asn Asn Lys Val Leu Val Tyr Arg Ser 
        595                 600                 605             


Glu Asn Gln Lys Lys Gly Asn Asn Thr Pro Tyr Met Tyr Leu Ser Lys 
    610                 615                 620                 


Gly Asp Lys Asn Trp Asn Tyr Asp Gln Tyr Lys Ser Tyr Val Leu Gly 
625                 630                 635                 640 


Leu Lys Asn Lys Gly Asn Ile Ser Asp Lys Lys Val Asn Asn Met Leu 
                645                 650                 655     


Phe Met Glu Asp Ile Asn Lys Ile Asp Val Leu Lys Gly Phe Ile Thr 
            660                 665                 670         


Arg Asn Leu Asn Asp Thr Arg Tyr Ala Ser Arg Val Val Leu Asn Met 
        675                 680                 685             


Leu Gln Ser Phe Phe Asn Glu Lys Asp Gly Cys Pro Thr Lys Val Lys 
    690                 695                 700                 


Val Ile Arg Gly Ser Phe Thr His Gln Met Arg Val Asn Leu Arg Ile 
705                 710                 715                 720 


Asn Lys Asp Arg Asp Glu Ser Tyr Ser His His Ala Val Asp Ala Met 
                725                 730                 735     


Leu Ile Val Tyr Ser Gln Arg Gly Tyr Glu Ala Tyr Arg Lys Ile Gln 
            740                 745                 750         


Glu Ala Cys Val Asp Phe Glu Thr Gly Glu Ile Ile Asp Glu Glu Arg 
        755                 760                 765             


Trp Gln Gln Cys Ile Trp Gly Lys Asp Tyr Asp Gln Val Met Tyr Glu 
    770                 775                 780                 


Ala Lys Trp Ser Lys Ile Arg Glu Lys Ile Ile Glu Ala Glu Lys Asn 
785                 790                 795                 800 


Val Lys Tyr His His Arg Val Asp Lys Lys Val Asn Arg Gly Leu Cys 
                805                 810                 815     


Asn Gln Thr Ile Tyr Gly Thr Arg Ile Lys Asp Gly Lys Ile Tyr Lys 
            820                 825                 830         


Ile Ser Ser Tyr Asn Ile Tyr Asp Asn Lys Glu Cys Ala Ser Leu Leu 
        835                 840                 845             


Lys Met Ile Lys Gly Gly Lys Glu Ser Lys Ile Leu Met Tyr Asn Tyr 
    850                 855                 860                 


Asp Ser Lys Thr Tyr Arg Asp Met Ile Lys Ile Ile Asp Glu Tyr Ser 
865                 870                 875                 880 


Asp Glu Lys Asn Pro Phe Val Ala Tyr Met Lys Glu Thr Gly Asp Tyr 
                885                 890                 895     


Phe Arg Lys Tyr Ala Lys Lys His Asn Gly Pro Lys Ile Glu Lys Val 
            900                 905                 910         


Lys Tyr Tyr Lys Glu Glu Ala Gly Ser Cys Ile Asp Ile Ser His Lys 
        915                 920                 925             


Tyr Gly Tyr Glu Lys Gly Ser Lys Lys Val Ile Leu Gly Ser Leu Asn 
    930                 935                 940                 


Pro Tyr Arg Thr Asp Val Phe Phe Asn Glu Arg Leu Lys Lys Tyr Tyr 
945                 950                 955                 960 


Leu Ile Gly Ile Lys Tyr Asn His Ile Lys Cys Glu Gly Asn Asn Tyr 
                965                 970                 975     


Val Ile Asp Glu Asp Ala Tyr Asn Gly Leu Leu Arg Asp Ala Gly Val 
            980                 985                 990         


Ile Ser His Glu Asn Thr Ile Asn  Asp Leu Glu Lys Val  Gly Val Lys 
        995                 1000                 1005             


Tyr Leu  Phe Ser Leu Tyr Lys  Asn Asp Ile Ile Lys  Tyr Glu Lys 
    1010                 1015                 1020             


Asn Gly  Glu Met Phe Val Glu  Arg Phe Leu Ser Arg  Thr Met Glu 
    1025                 1030                 1035             


Tyr Ala  Asn Asn Tyr Ile Glu  Thr Lys Pro Ile His  Lys Pro Phe 
    1040                 1045                 1050             


Phe Gln  Lys Lys Asp Lys Lys  Gly Asn Ile Lys Asn  Ser Gln Asn 
    1055                 1060                 1065             


Leu Val  Gly Leu Ser Lys Thr  Lys Tyr Ile Gly Lys  Leu Val Thr 
    1070                 1075                 1080             


Asp Ala  Leu Gly Asn Thr Tyr  Met Val Glu Lys Glu  Lys Phe Ser 
    1085                 1090                 1095             


Leu Ile  Val Asp Ile 
    1100             


<210>  643
<211>  1104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  643

Met Lys Leu Ile Leu Ala Leu Asp Ile Gly Ile Ala Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Leu Asp Lys Lys Ser Glu Thr Val Leu Glu Ala Gly Ser Asn 
            20                  25                  30          


Ile Phe Pro Glu Ala Ser Ala Ala Gly Asn Gln Val Arg Arg Glu Met 
        35                  40                  45              


Arg Gln Ala Arg Arg Ile Lys Arg Arg Gln Arg Thr Arg Leu Asp Asp 
    50                  55                  60                  


Phe Asn Lys Leu Trp Arg Lys Asn Asn Leu Ser Ile Pro Gln Tyr Lys 
65                  70                  75                  80  


Ser Thr Glu Ile Val Asn Leu Lys Val Lys Ala Leu Arg Glu Glu Ile 
                85                  90                  95      


Ser Leu Asp Glu Leu Tyr Leu Ile Leu Tyr Ser Tyr Leu Lys His Arg 
            100                 105                 110         


Gly Ile Ser Tyr Leu Glu Asp Ala Gln Asp Asp Ser Leu Ser Ser Ser 
        115                 120                 125             


Asn Ala Tyr Thr Asn Gly Leu Arg Glu Asn Ala Lys Ala Leu Glu Lys 
    130                 135                 140                 


His Tyr Pro Cys Glu Ile Gln Arg Asp Arg Leu Gln Thr Ile Gly Lys 
145                 150                 155                 160 


Tyr Arg Gly Gln Thr Gln Ile Thr Asp Asp Asn Asn Glu Lys Ile Asp 
                165                 170                 175     


Leu Ser Asn Val Phe Thr Ile Gly Ala Tyr Arg Lys Glu Ile Lys Lys 
            180                 185                 190         


Val Leu Glu Thr Gln Lys Leu Tyr His Asn Glu Leu Thr Asp Glu Leu 
        195                 200                 205             


Cys Asp Asp Tyr Ile Leu Ile Phe Asn Arg Lys Arg Lys Tyr Tyr Glu 
    210                 215                 220                 


Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe Thr Thr 
225                 230                 235                 240 


Arg Leu Asp Gln Asn Gly Asn Tyr Ile Asp Glu Glu Asn Ile Phe Glu 
                245                 250                 255     


Lys Leu Ile Gly Lys Cys Ser Val Tyr Pro Asp Glu Leu Arg Gly Ala 
            260                 265                 270         


Ala Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn 
        275                 280                 285             


Asn Leu Thr Ile Asn Gly Arg Lys Leu Glu Glu Ser Glu Lys His Ala 
    290                 295                 300                 


Ile Val Gln Arg Ile Lys Thr Ser Asn Thr Val Asn Met Arg Lys Ile 
305                 310                 315                 320 


Ile Ala Asp Cys Met Gly Glu Thr Ile Glu Asn Phe Ser Gly Ala Arg 
                325                 330                 335     


Ile Asp Lys Ser Gly Lys Glu Gln Phe His Lys Phe Glu Thr Tyr Asn 
            340                 345                 350         


Lys Met Arg Lys Ser Leu Ser Glu Ile Gly Thr Asp Ile Asp Glu Phe 
        355                 360                 365             


Ser Arg Asp Glu Leu Asp Lys Ile Gly Tyr Ile Met Thr Ile Asn Thr 
    370                 375                 380                 


Asp Lys Glu Ala Leu Met Glu Ala Phe Asn Glu Ser Gly Leu Asn Leu 
385                 390                 395                 400 


Asp Asp Ser Val Lys Glu Cys Leu Ile Ser Phe Arg Lys Ala Asn Ser 
                405                 410                 415     


Ser Ser Phe Ser Lys Trp Gln Ser Phe Ser Leu Lys Ile Met Asn Glu 
            420                 425                 430         


Leu Ile Pro Glu Met Tyr Ile Gln Pro Lys Glu Gln Met Thr Leu Leu 
        435                 440                 445             


Thr Glu Met Gly Val Ala Lys Gly Ser Ser Gly Asp Phe Ala Gly Leu 
    450                 455                 460                 


Asn Tyr Ile Pro Val Asp Val Ile Ser Asp Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Arg Arg Ser Val Arg Ile Ser Phe Lys Ile Leu Asn Ala Val Leu 
                485                 490                 495     


Lys Lys Tyr Lys Val Leu Asp Thr Ile Val Ile Glu Met Pro Arg Asp 
            500                 505                 510         


Arg Asn Ser Asp Glu Glu Arg Lys Arg Ile Thr Asp Ser Gln Lys Leu 
        515                 520                 525             


Asn Glu Lys Glu Met Glu Phe Ile Glu Gly Lys Leu Ala Gln Ile Tyr 
    530                 535                 540                 


Asn Ile Lys Leu Ser Ser Ser Asp Phe Ser Ser Gln Lys Gln Leu Ser 
545                 550                 555                 560 


Leu Lys Leu Lys Leu Trp Asn Glu Gln Asp Gly Ile Cys Leu Tyr Ser 
                565                 570                 575     


Gly Arg Thr Ile Asp Pro Glu Asp Ile Ile Lys Asn Pro Asp Leu Phe 
            580                 585                 590         


Glu Ile Asp His Ile Ile Pro Arg Ser Ile Ser Phe Asp Asp Ser Arg 
        595                 600                 605             


Ser Asn Lys Val Leu Val Tyr Arg Ser Glu Asn Gln Lys Lys Gly Asn 
    610                 615                 620                 


Gln Thr Pro Tyr Phe Tyr Ile Thr His Ser Asp Gly Ala Trp Thr Tyr 
625                 630                 635                 640 


Glu Gln Phe Lys Ala Asn Val Met Asn Leu Ser Lys Lys Lys Glu Tyr 
                645                 650                 655     


Ala Ile Ser Arg Lys Lys Val Gln Asn Leu Leu Tyr Ser Glu Asp Ile 
            660                 665                 670         


Thr Lys Arg Asp Val Leu Lys Gly Phe Ile Asn Arg Asn Ile Asn Asp 
        675                 680                 685             


Thr Gly Tyr Ala Ser Arg Val Val Leu Asn Thr Val Gln Asn Phe Phe 
    690                 695                 700                 


Lys Ala Lys Glu Ala Asp Thr Lys Val Lys Val Ile Lys Gly Ser Tyr 
705                 710                 715                 720 


Thr His Gln Met Arg Ser Asn Met Lys Leu Asp Lys Asn Arg Asp Glu 
                725                 730                 735     


Ser Tyr Ser His His Ala Val Asp Ala Met Leu Met Gly Phe Ser Glu 
            740                 745                 750         


Met Gly Tyr Glu Ala Tyr Arg Arg Leu Gln Gly Glu Phe Ile Asp Phe 
        755                 760                 765             


Glu Thr Gly Glu Ile Leu Lys Asn Asp Met Trp Asn Glu Lys Met Ser 
    770                 775                 780                 


Asp Asp Val Tyr Val Asp Tyr Leu Tyr Gly Arg Lys Trp Ser Ala Ile 
785                 790                 795                 800 


Arg Asn Glu Ile Val Lys Ala Glu Lys Asp Val Lys Phe Trp His Phe 
                805                 810                 815     


Ile Val Arg Lys Ser Asn Gln Gly Leu Cys Asn Gln Thr Ile Arg Gly 
            820                 825                 830         


Thr Arg Glu Tyr Asp Gly Lys Lys Tyr Lys Ile Asn Lys Phe Asp Ile 
        835                 840                 845             


Arg Thr Lys Glu Gly Val Lys Leu Phe Lys Lys Leu Ala Phe Ser Glu 
    850                 855                 860                 


Lys Glu Ser Asp Arg Glu Lys Leu Leu Val Tyr Leu Asn Asp Arg Gln 
865                 870                 875                 880 


Thr Phe Asn Asn Leu Val Lys Ile Phe Glu Asp Tyr Lys Asp Ala Pro 
                885                 890                 895     


Asn Pro Phe Val Gln Tyr Glu Lys Glu Thr Gly Asp Val Val Arg Lys 
            900                 905                 910         


Tyr Ser Lys Arg His Asn Gly Pro Arg Ile Asp Lys Leu Lys Tyr Lys 
        915                 920                 925             


Asp Gly Glu Val Gly Ala Cys Ile Asp Ile Ser His Lys Tyr Gly Leu 
    930                 935                 940                 


Glu Lys Asp Ser Arg Lys Val Ile Leu Glu Ser Leu Val Pro Tyr Arg 
945                 950                 955                 960 


Met Asp Val Tyr Tyr Lys Glu Ser Asp Lys Met Tyr Tyr Leu Val Gly 
                965                 970                 975     


Val Lys Gln Ser Asp Ile Lys Ser Gln Asn Gly Ala Asn Val Ile Asp 
            980                 985                 990         


Glu Asp Leu Tyr Thr Arg Ile Leu  Ile Asn Glu Lys Met  Ile Glu Pro 
        995                 1000                 1005             


Gly Gln  Ser Arg Asp Asp Leu  Glu Gln Leu Gly Tyr  Glu Phe Arg 
    1010                 1015                 1020             


Phe Ser  Phe Tyr Lys Asn Asp  Ile Ile Glu Tyr Glu  Lys Glu Gly 
    1025                 1030                 1035             


Lys Val  Phe Val Glu Arg Phe  Val Ser Arg Thr Met  Pro Lys Val 
    1040                 1045                 1050             


Arg Asn  Tyr Ile Glu Thr Lys  Pro Ile Asn Lys Ala  Asn Phe Glu 
    1055                 1060                 1065             


Lys Gln  Asn Pro Val Gly Leu  Gly Lys Thr Lys Ser  Ile Lys Lys 
    1070                 1075                 1080             


Tyr Arg  Thr Asp Ile Leu Gly  Asn Arg Tyr Leu Cys  Glu Lys Glu 
    1085                 1090                 1095             


Lys Phe  Thr Lys Phe Cys 
    1100                 


<210>  644
<211>  1104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  644

Met Asn His Tyr Val Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly 
1               5                   10                  15      


Tyr Gly Val Ile Asp Leu Asp Asn Tyr Gln Phe Val Asp Tyr Gly Val 
            20                  25                  30          


Arg Leu Phe Lys Glu Gly Thr Ala Glu Asp Asn Val Asn Arg Arg Thr 
        35                  40                  45              


Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Gln Thr Arg Leu Asp 
    50                  55                  60                  


Asp Met Lys Glu Leu Leu Gln Lys Glu Lys Ile Met Ser Glu Thr Tyr 
65                  70                  75                  80  


His Ser Ser Tyr Asp Pro Tyr Asp Met Arg Ile Lys Gly Leu Ser Gln 
                85                  90                  95      


Lys Leu Ser Asn Asp Glu Leu Thr Cys Ala Ile Leu His Ile Thr Lys 
            100                 105                 110         


Cys Arg Gly Thr Thr Leu Glu Ala Leu Asp Ser Ala Asp Glu Asp Ser 
        115                 120                 125             


Glu Gly Thr Lys Ser Ile Leu Ser Lys Asn Asp Gln Asp Leu Lys Lys 
    130                 135                 140                 


Tyr His Tyr Val Cys Leu Val Gln Lys Glu Arg Leu Lys Gln Ser Gly 
145                 150                 155                 160 


Lys Ile Arg Gly Thr Glu Asn Asn Phe Lys Thr Asp Asp Tyr Ile Lys 
                165                 170                 175     


Glu Leu Asp Glu Ile Leu Ser His Gln Asp Leu Ser Glu Asp Leu Cys 
            180                 185                 190         


Glu Lys Ile Arg Thr Ile Val Ser Arg Arg Arg Gln Tyr Tyr Glu Gly 
        195                 200                 205             


Pro Gly Ser Glu Lys Ser Pro Thr Pro Tyr Gly Arg Trp Ile Ala Gln 
    210                 215                 220                 


Gly Val Ala Pro Ile Asp Leu Ile Glu Lys Met Arg Gly Lys Cys Ser 
225                 230                 235                 240 


Val Phe Pro Asp Gln Leu Arg Ala Ala Lys Asn Ser Tyr Thr Ala Glu 
                245                 250                 255     


Leu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Glu Cys Glu Gly Glu 
            260                 265                 270         


Lys Leu Thr Thr Ser Gln Lys Gln Asp Val Ile Asp Phe Val Asn Glu 
        275                 280                 285             


Lys Gly Gly Ile Thr Pro Lys Gln Leu Ala Lys Leu Leu Asn Val Asp 
    290                 295                 300                 


Leu Asn Gln Ile Lys Gly Phe Arg Ile Asp Lys Asn Asp Lys Pro Leu 
305                 310                 315                 320 


Leu Thr Glu Phe Lys Gly Tyr Lys Ala Phe Lys Lys Ile Phe Asp Thr 
                325                 330                 335     


Asn Asn Cys Asp Leu Tyr Lys Lys Asp Leu Ser Ile Leu Asp Gly Leu 
            340                 345                 350         


Ala Glu Ile Val Thr Gly Lys Lys Gly Ile Glu Glu Arg Lys Gln Ala 
        355                 360                 365             


Ile Gln Gln Ala Tyr Pro Glu Ile Asp Glu Ala Ile Val Glu Gln Cys 
    370                 375                 380                 


Ala Val Met Lys Gly Met Thr Gln Tyr His Ser Leu Ser Leu Lys Ala 
385                 390                 395                 400 


Met His Leu Ile Asn Gln Glu Met Leu Gln Ser Pro Arg Asn Gln Met 
                405                 410                 415     


Gln Val Leu His Glu Ser His Val Phe Asp Lys Asn Arg Val Ser His 
            420                 425                 430         


Lys Gly Gln Lys Tyr Ile Glu Ala Asp Asp Glu Ala Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ala Gln Arg Glu Thr Phe Lys Val Val Asn Ala Leu 
    450                 455                 460                 


Arg Glu Lys Tyr Gly Glu Phe Glu Ser Ile Val Val Glu Thr Thr Arg 
465                 470                 475                 480 


Asp Arg Asn Ser Gln Glu Gln Lys Lys Arg Leu Asn Asp Ser Gln Lys 
                485                 490                 495     


Tyr Tyr Glu Gln Glu Asn Arg Lys Val Asp Glu Leu Leu Ser His Glu 
            500                 505                 510         


Gly Tyr Asp Pro Ser Lys Ile Asn Gly Lys Thr Lys Leu Lys Val Arg 
        515                 520                 525             


Leu Tyr Leu Gln Gln Glu Gly Lys Thr Ala Tyr Thr Asn Ala Pro Ile 
    530                 535                 540                 


Asp Leu Asn Leu Leu Ile Lys Asp Ala Thr Ala Tyr Glu Val Asp His 
545                 550                 555                 560 


Ile Ile Pro Leu Ser Ile Ser Leu Asp Asp Ser Ile Asn Asn Lys Ala 
                565                 570                 575     


Leu Ile Asn Arg Thr Glu Asn Gln Asn Lys Ser Asn Met Thr Pro Ile 
            580                 585                 590         


Tyr Ala Ile Leu Arg Gly Lys Leu Asn Ile Ser Leu Asp Thr Tyr Lys 
        595                 600                 605             


Thr Val Val Lys Asn Asn Arg Asn Tyr Thr Gly Lys Lys Arg Ser Tyr 
    610                 615                 620                 


Leu Leu Tyr Asp Lys Asp Ile Thr Lys Phe Ser Asn Met Gln Glu Phe 
625                 630                 635                 640 


Ile Ala Arg Asn Leu Val Asp Thr Ser Tyr Ala Cys Arg Val Val Met 
                645                 650                 655     


Ser Thr Leu Ser Asp Tyr Phe Lys Asp Asn Glu Ile Ser Thr His Val 
            660                 665                 670         


His Thr Val Arg Gly Arg Val Thr Asn Met Phe Arg Asn Arg Ile Asn 
        675                 680                 685             


Met Gln Lys Glu Arg Glu Lys Asp Tyr Leu His His Ala Val Asp Ala 
    690                 695                 700                 


Leu Ile Val Ala Ser Ile Lys Lys Leu Asn Leu Leu Asn Thr Tyr Leu 
705                 710                 715                 720 


Leu Lys Tyr Asn Phe Asn Asp Leu Tyr Asn Glu Glu Thr Gly Glu Val 
                725                 730                 735     


Phe Ala Val Gly Asp Asp Gln Gln Val Leu Asp Pro Lys Tyr Ile Arg 
            740                 745                 750         


Phe Ile Thr Gln Leu Lys Thr Ile Tyr Asp Glu Ser Asn Met Tyr Tyr 
        755                 760                 765             


Asn Gly Leu Ile Glu Arg Asn Val Met Gln Phe Pro Pro Ile Lys Ile 
    770                 775                 780                 


Ser His Lys Ile Asp Thr Lys Pro Asn Arg Gln Ile Ser Asp Glu Thr 
785                 790                 795                 800 


Ile Tyr Ser Thr Arg Thr Val Gly Asn Glu Glu Tyr Val Val Lys Lys 
                805                 810                 815     


Tyr Ser Asp Ile Tyr Asp Pro Lys Phe Thr Gln Leu Thr Asp Asp Ile 
            820                 825                 830         


Ile Asn Asp Arg Pro Leu Asp Arg Trp Leu Met Tyr Arg Asn Asp Asn 
        835                 840                 845             


Gln Thr Phe Lys Ile Ile Gln Ser Ile Ile Leu Asp His Phe His Ile 
    850                 855                 860                 


Phe Lys Asp Asp Glu Lys His Tyr Ser Cys Thr Lys Lys Lys Gly Gln 
865                 870                 875                 880 


Ile Gln Tyr Ala Leu Lys Gly Lys Asn Pro Leu Tyr Leu Tyr Lys Gln 
                885                 890                 895     


Glu His Gly Lys Val Arg Lys Tyr Ala Lys Lys Gly Asn Gly Pro Glu 
            900                 905                 910         


Ile Thr Met Met Lys Tyr Phe Asp Gly Lys Leu Gly Asn Asn Ile Asp 
        915                 920                 925             


Ile Ser Asn His Tyr His Val His Asn Lys Lys Val Val Leu Leu Gln 
    930                 935                 940                 


Ile Ser Pro Tyr Arg Thr Asp Phe Tyr Val Ser Pro Glu Gly Lys Tyr 
945                 950                 955                 960 


Lys Met Leu Thr Val Arg Tyr Ala Asn Val Phe Tyr Lys Glu Ser Lys 
                965                 970                 975     


Lys Lys Tyr Val Ile Asp Glu Lys Trp Tyr Ala Ser Glu Lys Lys Arg 
            980                 985                 990         


Lys Asn Ile Thr Asp Asp Asp Ile  Phe Val Cys Ser Leu  His His Asp 
        995                 1000                 1005             


Glu Leu  Ile Gly Ile Gln Lys  Lys Asp Gly Ala Lys  Tyr Val Tyr 
    1010                 1015                 1020             


Asp Leu  Ser Thr Glu Asp Gly  Gly Lys Pro Arg Leu  Tyr His Gly 
    1025                 1030                 1035             


Glu Met  Glu Ile Leu Lys Phe  Thr Ala Thr Asn Asn  Asp Ser Lys 
    1040                 1045                 1050             


Asn Ile  Ile Glu Val Lys Pro  Ile Tyr Thr Tyr Cys  Lys Lys Gln 
    1055                 1060                 1065             


Leu Met  Ile Ser Ile Gly Pro  Ile Thr Lys Leu Ala  Lys Tyr Ala 
    1070                 1075                 1080             


Thr Asp  Val Leu Gly Asn Val  Tyr Glu Val Lys Lys  Asn Ile Ile 
    1085                 1090                 1095             


Asp His  Leu Ser His Leu 
    1100                 


<210>  645
<211>  1105
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  645

Met Gly Ile Arg Ile Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Val Asp Asp Asp Tyr Asn Ile Ile Glu Ser Gly Ala Asn Ile 
            20                  25                  30          


Phe Asn Glu Ala Lys Ala Ala Ser Asn Val Asp Arg Arg Thr Phe Arg 
        35                  40                  45              


Gln Thr Lys Arg Leu Leu Arg Arg Gln Lys Thr Arg Leu Arg Asp Phe 
    50                  55                  60                  


Asp Ile Leu Trp Ile Asn Ala Gly Leu Asp Ile Pro Lys Glu Lys Val 
65                  70                  75                  80  


Asn Lys Gln Leu Gln Phe Arg Val Gln Gly Met Thr Met Glu Leu Ser 
                85                  90                  95      


Glu Asn Glu Leu Tyr Ala Ala Leu Lys Asn Met Leu Lys His Arg Gly 
            100                 105                 110         


Ile Ser Tyr Leu Glu Asp Ala Leu Asp Glu Glu Gln Ser Gly Lys Ser 
        115                 120                 125             


Thr Tyr Glu Lys Gly Leu Gln Val Asn Gln Lys Ala Leu Lys Cys Asp 
    130                 135                 140                 


Lys Met Pro Cys Glu Ile Gln Tyr Glu Arg Leu Met Lys Tyr Gly Ser 
145                 150                 155                 160 


Tyr Arg Gly His Ile Glu Val Lys Asp Glu Ser Gly Glu Glu Met Ile 
                165                 170                 175     


Leu Ser Asn Ile Phe Thr Leu Asp Ala Tyr Cys Lys Glu Ala Lys Lys 
            180                 185                 190         


Ile Leu Glu Cys Gln Lys Gln Tyr His Ser Phe Ile Thr Glu Ala Phe 
        195                 200                 205             


Glu Asp Asp Tyr Leu Ala Leu Leu Arg Arg Lys Arg Lys Tyr Tyr Glu 
    210                 215                 220                 


Gly Pro Gly Asn Glu Gln Ser Arg Thr Asp Tyr Gly Lys Tyr Thr Thr 
225                 230                 235                 240 


Lys Leu Asn Pro Glu Thr Gly Glu Tyr Val Thr Glu Glu Asn Ile Phe 
                245                 250                 255     


Glu Lys Leu Ile Gly Lys Cys Ser Val Tyr Pro Lys Glu Met Arg Ala 
            260                 265                 270         


Ser Ala Ala Ser Tyr Thr Ala Gln Ile Tyr Asn Met Leu Asn Asp Leu 
        275                 280                 285             


Asn Asn Leu Thr Ile Asn Gly Arg Lys Leu Glu Tyr Glu Glu Lys Lys 
    290                 295                 300                 


Ser Ile Val Glu Glu Val Gln Lys Ala Ser Ser Val Asn Met Leu Lys 
305                 310                 315                 320 


Ile Ile Lys Lys Val Ile Asn Glu Asp Ile Thr Thr Ile Glu Gly Ala 
                325                 330                 335     


Arg Lys Asp Lys Asn Asp Lys Glu Ile Phe His Lys Met Glu Thr Tyr 
            340                 345                 350         


Asn Lys Met Arg His Tyr Phe Glu Lys Asn Gly Leu Glu Phe Lys Lys 
        355                 360                 365             


Ile Ser Thr Asp Asp Leu Asp Glu Ile Gly Asn Ile Leu Thr Leu Asn 
    370                 375                 380                 


Thr Glu Lys Glu Ala Ile Leu Glu Ala Phe Asn Lys Ser Gln Leu Glu 
385                 390                 395                 400 


Leu Ser Gln Glu Glu Lys Glu Ala Phe Ile Glu Ile Arg Lys Lys Asn 
                405                 410                 415     


Gly Ser Leu Phe Asn Lys Trp His Ser Phe Ser Leu Lys Ile Met Lys 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Leu Gln Ser Lys Asn Gln Met Gln Leu 
        435                 440                 445             


Leu Thr Asp Met Gly Val Phe Lys Lys Lys Thr Glu Ile Phe Gln Asn 
    450                 455                 460                 


Tyr Asp Lys Ile Pro Thr Asp Val Ile Ile Glu Asp Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Lys Arg Ser Ile Arg Ile Thr Val Lys Ile Val Asn Ala Leu 
                485                 490                 495     


Ile Lys Lys Tyr Gly Tyr Pro Arg Glu Val Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Arg Asn Ser Asp Glu Glu Lys Lys Arg Ile Gln Asp Glu Gln Lys 
        515                 520                 525             


Lys Asn Asp Thr Glu Leu Lys Lys Ile Ile Glu Lys Val Glu Ser Glu 
    530                 535                 540                 


Tyr Gly Ile Lys Ile Thr Asp Thr His Phe Arg Asn His Lys Asn Leu 
545                 550                 555                 560 


Arg Met Lys Leu Arg Leu Trp His Glu Gln Asp Gly Arg Cys Leu Tyr 
                565                 570                 575     


Ser Gly Lys Val Ile Asp Ile Ser Glu Leu Leu Asn Asp Cys Ser Lys 
            580                 585                 590         


Phe Glu Ile Asp His Val Ile Pro Lys Ser Ile Ser Phe Asp Asp Ser 
        595                 600                 605             


Arg Ser Asn Lys Val Leu Val Tyr Ser Thr Glu Asn Gln Lys Lys Thr 
    610                 615                 620                 


Asn Gln Thr Pro Tyr Gln Tyr Leu Glu Gly Asn Gly Lys Glu Trp Ser 
625                 630                 635                 640 


Phe Asp Glu Tyr Met Asn Gln Val Ile Glu Leu Lys Asp Arg Lys Leu 
                645                 650                 655     


Ile Ser Lys Ala Lys Met Asn Lys Leu Leu Phe Arg Glu Asp Ile Thr 
            660                 665                 670         


Lys Ile Glu Val Leu Lys Gly Phe Ile Ala Arg Asn Ile Asn Asp Thr 
        675                 680                 685             


Arg Tyr Ala Ser Arg Val Val Leu Asn Ser Phe Gln Lys Tyr Phe Glu 
    690                 695                 700                 


Ser Lys Gln Ser Asp Thr Lys Val Lys Val Ile Arg Gly Ser Phe Thr 
705                 710                 715                 720 


His Gln Met Arg Lys Lys Leu Gln Leu Ser Lys Asn Arg Asp Glu Ser 
                725                 730                 735     


Tyr Ala His His Ala Val Asp Ala Met Leu Ile Cys Tyr Ser Gln Met 
            740                 745                 750         


Gly Tyr Glu Ala Tyr Arg Gln Leu Gln Glu Asp Leu Ile Asp Pro Asp 
        755                 760                 765             


Ser Glu Lys Ile Arg Asp Leu Gly Leu Trp Lys Glu Thr Ile Thr Glu 
    770                 775                 780                 


Ala Lys Tyr Asp Glu Val Leu Tyr Thr Ala Lys Trp Leu Glu Asn Arg 
785                 790                 795                 800 


Gly Lys Ile Gln Glu Ala Glu Lys Lys Val Lys Phe Trp His Lys Val 
                805                 810                 815     


Asp Ser Lys Ala Asn Arg Ala Leu Cys Asn Gln Thr Ile Arg Gly Thr 
            820                 825                 830         


Arg Val Ile Asp Gly Ser Ile Gln Lys Ile Asn Lys Ile Asn Ile Tyr 
        835                 840                 845             


Ser Val Asp Gly Tyr Lys Thr Phe Lys Asn Met Ile Gln Lys Gly Lys 
    850                 855                 860                 


Glu Lys Asp Phe Leu Met Tyr Arg Asn Asp Arg Arg Ser Trp Asp Asp 
865                 870                 875                 880 


Leu Met Lys Val Tyr Arg Glu Tyr Ser Asp Ala Lys Asn Ala Phe Ala 
                885                 890                 895     


Ala Tyr Gln Glu Glu Thr Gly Asp Tyr Val Arg Lys Tyr Ala Lys Lys 
            900                 905                 910         


His Asn Gly Ser Lys Ile Thr Asn Leu Lys Tyr Cys Ala Lys Glu Leu 
        915                 920                 925             


Gly Ser Cys Ile Asp Ile Ser His Lys Tyr Gly Leu Glu Lys Asp Ser 
    930                 935                 940                 


Arg Lys Val Ile Leu Glu Ser Leu Ser Pro Tyr Arg Ser Asp Val Tyr 
945                 950                 955                 960 


Tyr Asn Ala Gly Leu Lys Gln Tyr Tyr Ile Val Gly Ile Lys Asn Ser 
                965                 970                 975     


Asp Leu Cys Phe Leu Lys Gly Glu Tyr Phe Ile Asp Ser Lys Arg Tyr 
            980                 985                 990         


Leu Gln Ile Leu Arg Glu Glu Gly  Val Leu Thr Glu Gln  His Thr Leu 
        995                 1000                 1005             


Asn Asp  Leu Ala Ala Ile Gly  Thr Glu Phe Gln Phe  Ser Leu Tyr 
    1010                 1015                 1020             


Glu Asn  Asp Tyr Leu Glu Tyr  Glu Lys Asn Gly Glu  Ile Lys Thr 
    1025                 1030                 1035             


Glu Arg  Phe Leu Ser Arg Thr  Lys Pro Lys Gln Lys  Asn Tyr Ile 
    1040                 1045                 1050             


Glu Thr  Lys Ser Ile Asp Ala  Pro Lys Ile Lys Asn  Lys Lys Gln 
    1055                 1060                 1065             


Asn Leu  Val Gly Leu Ala Lys  Ser Lys Ser Ile Lys  Lys Ile Arg 
    1070                 1075                 1080             


Val Asp  Ile Leu Gly Asn Arg  Tyr Tyr Ala Ser Lys  Glu Lys Phe 
    1085                 1090                 1095             


Ser Leu  Met Val Asp Lys Phe  
    1100                 1105 


<210>  646
<211>  1105
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  646

Met Asp Met Ala Trp Arg Leu Gly Leu Asp Leu Gly Thr Asn Ser Leu 
1               5                   10                  15      


Gly Trp Ala Ala Leu Ser Leu Asp Ala Ala Gly Ala Pro Asp Ala Ile 
            20                  25                  30          


Leu Ala Ala Gly Ser Arg Ile Phe Gly Asp Gly Arg Asp Pro Gln Ser 
        35                  40                  45              


Gly Thr Ser Leu Ala Val Asp Arg Arg Ala Ala Arg Ala Ala Arg Arg 
    50                  55                  60                  


Arg Arg Asp Arg Phe Lys Gln Arg Gln Arg Ala Leu Leu Lys His Leu 
65                  70                  75                  80  


Glu Ala Asp Gly Leu Phe Pro Ala Asp Pro Glu Val Gln Gln Ala Leu 
                85                  90                  95      


Ala Ala Leu Asp Pro Tyr Ala Leu Arg Ala Arg Ala Leu Asp Glu Ala 
            100                 105                 110         


Leu Ser Leu His Glu Ile Gly Arg Ala Leu Phe His Leu Asn Gln Arg 
        115                 120                 125             


Arg Gly Phe Gln Ser Asn Arg Lys Ala Asp Arg Gly Lys Asp Glu Asp 
    130                 135                 140                 


Ala Gly Lys Ile Ala Ile Gly Val Asp Arg Leu Lys Asp Ala Ile Ala 
145                 150                 155                 160 


Ala Ala Gly Ala Arg Thr Phe Gly Glu Phe Leu Arg Gln Arg Arg Ala 
                165                 170                 175     


Gly Ala Thr Gly Glu Asn Gln Ile Pro Ser Val Arg Thr Arg Leu Arg 
            180                 185                 190         


Ala Glu Thr Gly Glu Gly Ala Lys Gly Ser Gly Tyr Asp Phe Tyr Pro 
        195                 200                 205             


Ser Arg Ala Leu Leu Lys Asp Glu Phe Asp Ala Ile Trp His Ala Gln 
    210                 215                 220                 


Ala Glu His His Pro Lys Val Leu Thr Asp Glu Ala Tyr His Arg Leu 
225                 230                 235                 240 


His Glu Ile Val Phe Arg Gln Arg Pro Leu Arg Ala Pro Lys Val Gly 
                245                 250                 255     


Ala Cys Thr Leu Val Pro Gly Glu Ala Arg Leu Pro Lys Ala His Pro 
            260                 265                 270         


Leu Phe Gln Arg Arg Arg Leu Leu Glu Glu Leu Asn Ala Leu Met Ile 
        275                 280                 285             


Val Arg Ala Gly Ala Val Ala Glu Arg Leu Thr Pro Glu Gln Arg Asp 
    290                 295                 300                 


Leu Leu Leu Leu Lys Leu Lys Asp Lys Gly Lys Val Thr Phe Lys Ser 
305                 310                 315                 320 


Leu Arg Glu Lys Val Leu Lys Leu Asp Gly Asp Ala Arg Phe Asn Lys 
                325                 330                 335     


Glu Ser Glu His Arg Thr Glu Leu Lys Gly Asp Glu Val Ala Ala Glu 
            340                 345                 350         


Met Gly Gly Lys Thr Arg Phe Gly Ser Leu Trp Pro His Leu Ser Thr 
        355                 360                 365             


Glu Gln Gln Trp Thr Val Ile Glu Arg Gln Gln Ala Leu Glu Ser Asp 
    370                 375                 380                 


Ala Asp Glu Ala Ala Phe Arg Thr Trp Leu Val Glu Thr His Lys Leu 
385                 390                 395                 400 


Thr Ala Glu Gln Ala Arg Ala Val Ala Gly Ala Arg Leu Pro Ala Gly 
                405                 410                 415     


His Gly Arg Phe Gly Leu Thr Ala Thr Ala Ala Leu Leu Ala Ala Leu 
            420                 425                 430         


Arg Asp Gly Arg Thr Pro Glu Gly Arg Gly Pro Asn Ala Pro Ala Thr 
        435                 440                 445             


Glu Ala Gly Arg Val Val Val Tyr Ser Glu Ala Ala Ala Ile Ala Gly 
    450                 455                 460                 


Tyr His His Ser Asp His Arg Ser Gly Glu Val Phe Thr Asp Ala Lys 
465                 470                 475                 480 


Gly Arg Pro Ala Leu Pro Tyr Tyr Gly Val Pro Leu Asp Arg His Ile 
                485                 490                 495     


Val Pro Gly Thr Ala Asp Pro Asp Glu Pro Asp Glu Ala Ala Arg Ile 
            500                 505                 510         


Gly Arg Leu Thr Asn Pro Thr Val His Ile Ala Leu Asn Gln Leu Arg 
        515                 520                 525             


Arg Val Val Asn Arg Leu Ile Arg Val Tyr Gly Pro Pro Ala Glu Val 
    530                 535                 540                 


Ala Leu Glu Leu Ala Arg Glu Leu Lys Leu Ser Glu Asp Glu Lys Lys 
545                 550                 555                 560 


Glu Arg Asn Arg Glu Asn Ser Arg Asn Arg Leu Asp Ala Glu Lys Arg 
                565                 570                 575     


Ser Ser Lys Leu Ala Glu Leu Gly Gln Arg Asp Asn Gly Gly Asn Arg 
            580                 585                 590         


Ala Leu Leu Lys Leu Trp Glu Glu Leu Asn Pro Glu Asn Ile Leu Asp 
        595                 600                 605             


Arg Arg Cys Ile Tyr Ser Gly Arg Gln Ile Ser Ile Gly Met Ile Phe 
    610                 615                 620                 


Ser Gly Ala Ala Glu Val Asp His Ile Leu Pro Phe Asp Ala Thr Leu 
625                 630                 635                 640 


Asp Asp Ser Asn Ala Asn Lys Ile Leu Cys Leu Arg Glu Ala Asn Arg 
                645                 650                 655     


Asp Lys Arg Lys Arg Ser Pro Phe Glu Ala Trp Gly Gly Thr Pro Gln 
            660                 665                 670         


Trp Glu Glu Ile Ala Glu Arg Ala Ser Arg Leu Pro Arg Asn Lys Arg 
        675                 680                 685             


Trp Arg Phe Glu Pro Asp Ala Met Glu Arg Phe Ala Glu Glu Gly Gly 
    690                 695                 700                 


Phe Leu Ala Arg His Leu Val Asp Thr Gln Tyr Leu Gly Arg Ile Ala 
705                 710                 715                 720 


His Asp Tyr Leu Arg Cys Leu Tyr Pro Asp Lys Gly Asp Gly Ser Ser 
                725                 730                 735     


His Val Trp Val Ser Pro Gly Arg Leu Thr Glu Met Val Arg Arg Lys 
            740                 745                 750         


Leu Gly Leu Asn Gly Leu Leu Gly Asp His Asn Leu Gly Ala Glu Gln 
        755                 760                 765             


Pro Lys Asn Arg Lys Asp His Arg His His Ala Ile Asp Ala Val Val 
    770                 775                 780                 


Thr Ala Ile Leu Asp Arg Ser Met Leu Gln Arg Ile Gln Arg Ala Ser 
785                 790                 795                 800 


Gly Glu Gly Ala Ser Asp Ala Glu Leu Leu Arg Ile Ile Val Pro Glu 
                805                 810                 815     


Pro Trp Thr Gly Phe Arg Asp Asp Leu Gln Arg Ala Val Asp Arg Ile 
            820                 825                 830         


Val Val Ala His Arg Ala Asp His Gly Thr Val Ala Lys Ala Ala Thr 
        835                 840                 845             


Arg Gly Arg Asp Gln Thr Ala Ala Arg Leu His Asn Asp Thr Ala Tyr 
    850                 855                 860                 


Gly Phe Thr Gly Glu Ala Asp Ala Lys Gly Thr Pro Ile Val Val His 
865                 870                 875                 880 


Arg Ala Pro Leu Gly Ala Leu Lys Lys Pro Glu His Ile Asp Gln Val 
                885                 890                 895     


Arg Asp Pro Leu Leu Arg Ala Ala Leu His Asp Phe Thr Ala Gly Leu 
            900                 905                 910         


Ser Gly Lys Pro Phe Glu Asp Arg Ile Ala Ala Phe Pro Arg Leu Gly 
        915                 920                 925             


Pro Leu Asp Tyr Arg Gly Ile Arg Arg Val Arg Val Val Glu Pro Leu 
    930                 935                 940                 


Gln Val Ile Pro Ile Arg Asp Ala Ala Gly Arg Ala Tyr Lys Gly Tyr 
945                 950                 955                 960 


Lys Gly Asp Ser Asn Tyr Arg Tyr Asp Val Trp Glu Leu Pro Asp Gly 
                965                 970                 975     


Lys Trp Glu Gln Arg Val Leu Gln Met Tyr Tyr Ala His Gln Ser Gly 
            980                 985                 990         


Glu Pro Pro Arg Pro His Pro Ala  Ala Arg Lys Val Leu  Ser Leu His 
        995                 1000                 1005             


Arg Asp  Asp Val Leu Ala Ile  Glu Arg Gly Glu Gly  Gly Arg Glu 
    1010                 1015                 1020             


Leu Val  Arg Val Val Lys Phe  Ser Thr Asn Glu Phe  Thr Leu Ala 
    1025                 1030                 1035             


Pro Leu  Asn Glu Gly Gly Ala  Leu Lys Ser Arg His  Ala Asp Lys 
    1040                 1045                 1050             


Gly Asp  Pro Phe Arg Tyr Leu  Tyr Pro Ser Pro Ser  Thr Leu Lys 
    1055                 1060                 1065             


Ala Trp  Arg Ala Arg Glu Val  Arg Val Asp Glu Leu  Gly Arg Val 
    1070                 1075                 1080             


Leu Asp  Pro Gly Phe Pro Ala  Arg Lys Arg Arg Arg  Val Thr Arg 
    1085                 1090                 1095             


Pro Gly  Arg Ala Asp Ala Asp  
    1100                 1105 


<210>  647
<211>  1106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  647

Met Gln Tyr Ile Val Ala Ile Asp Ile Gly Val Ala Ser Val Gly Tyr 
1               5                   10                  15      


Ala Val Ile Asp Lys Thr Ser Glu Thr Val Ile Glu Ala Gly Ser Asn 
            20                  25                  30          


Ile Phe Pro Glu Ala Ser Ala Thr Asp Asn Gln Glu Arg Arg Gly Met 
        35                  40                  45              


Arg Gln Ala Arg Arg Leu Lys Arg Arg Glu Arg Thr Arg Leu Asp Asp 
    50                  55                  60                  


Phe Thr Lys Leu Trp Glu Tyr His Gly Leu Ala Ile Pro Gln Val Lys 
65                  70                  75                  80  


Lys Asn Asp Ile Val Gly Leu Lys Val Lys Ala Leu Tyr Glu Ser Ile 
                85                  90                  95      


Ser Leu Asp Glu Val Tyr Leu Ile Leu Tyr Ser Tyr Leu Lys His Arg 
            100                 105                 110         


Gly Ile Ser Tyr Leu Glu Asp Val Glu Asp Ser Ser Val Ser Gly Ser 
        115                 120                 125             


Thr Ala Tyr Ala Asn Gly Leu Lys Leu Asn Ala Lys Glu Leu Glu Lys 
    130                 135                 140                 


Asn Phe Pro Cys Glu Ile Gln Lys Tyr Arg Leu Glu Asn Ile Gly Lys 
145                 150                 155                 160 


Tyr Arg Gly Gln Ile Gln Ile Glu Asp Asp Gln Gly Glu Lys Leu Asp 
                165                 170                 175     


Leu Ser Asn Val Phe Thr Ile Gly Ala Tyr Thr Lys Glu Ile Gln Gln 
            180                 185                 190         


Ile Leu Asp Thr Gln Lys Lys Tyr His Ala Glu Ile Glu Asp Glu Phe 
        195                 200                 205             


Cys Glu Lys Tyr Leu Lys Ile Phe Thr Arg Lys Arg Lys Tyr Tyr Glu 
    210                 215                 220                 


Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Lys Tyr Thr Thr 
225                 230                 235                 240 


Arg Leu Lys Glu Asn Gly Glu Tyr Ile Thr Glu Lys Asn Ile Phe Glu 
                245                 250                 255     


Lys Leu Ile Gly Lys Cys Ser Val Tyr Lys Asp Glu Leu Arg Ala Ala 
            260                 265                 270         


Ser Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn 
        275                 280                 285             


Asn Leu Thr Val Asn Gly Arg Lys Leu Glu Glu Asn Glu Lys His Leu 
    290                 295                 300                 


Ile Val Asp Lys Val Lys Thr Ser Asn Thr Ile Asn Met Arg Lys Ile 
305                 310                 315                 320 


Ile Ala Asp Ala Met Gly Glu Lys Ile Glu Glu Phe Tyr Gly Ala Arg 
                325                 330                 335     


Ile Asp Lys Ser Glu Lys Glu Ile Phe His Lys Phe Glu Val Tyr Asn 
            340                 345                 350         


Lys Met Arg Lys Ala Leu Ala Ala Ile His Val Asn Ile Glu Glu Phe 
        355                 360                 365             


Ser Arg Asp Asp Leu Asp Glu Ile Gly Tyr Ile Leu Thr Ile Asn Thr 
    370                 375                 380                 


Asp Lys Glu Ala Met Met Glu Ala Phe Asp Asn Ser Arg Leu Glu Leu 
385                 390                 395                 400 


Pro Gly Glu Ile Lys Asn Cys Leu Ile Ser Leu Arg Lys Thr Asn Gly 
                405                 410                 415     


Thr Leu Tyr Ser Lys Trp His Ser Phe Ser Leu Lys Ile Met Asn Glu 
            420                 425                 430         


Leu Ile Pro Glu Met Tyr Arg Gln Pro Lys Glu Gln Met Thr Leu Leu 
        435                 440                 445             


Thr Glu Met Gly Val Met Lys Gly Gln Thr Asp Glu Phe Glu Lys Asn 
    450                 455                 460                 


Lys Tyr Ile Pro Val Asp Val Ala Ser Glu Asp Ile Phe Asn Pro Val 
465                 470                 475                 480 


Val Arg Arg Ser Val Arg Ile Ser Phe Lys Ile Leu Asn Ala Leu Met 
                485                 490                 495     


Lys Lys Tyr Gly Thr Leu Asp Glu Val Val Ile Glu Met Pro Arg Asp 
            500                 505                 510         


Arg Asn Ser Asp Glu Gln Lys Lys Trp Glu Lys Glu Thr Gln Lys Lys 
        515                 520                 525             


Asn Glu Lys Glu Gln Glu Tyr Ile Glu Asn Lys Leu Ala Thr Val Tyr 
    530                 535                 540                 


Asp Ile Arg Leu Ser Ser Ala Asp Phe Ser Ser Gln Lys Gln Leu Asn 
545                 550                 555                 560 


Leu Lys Leu Lys Leu Trp Asn Glu Gln Asp Gly Lys Cys Leu Tyr Ser 
                565                 570                 575     


Gly Lys Ile Ile Glu Pro Glu Asp Leu Ile Lys His Ser Glu Leu Phe 
            580                 585                 590         


Glu Ile Asp His Ile Ile Pro Arg Ser Ile Ser Phe Asp Asp Ser Arg 
        595                 600                 605             


Ser Asn Lys Val Leu Val Tyr Arg Thr Glu Asn Gln Asn Lys Gly Asn 
    610                 615                 620                 


Gln Thr Pro Tyr Tyr Tyr Leu Thr His Thr Asn Ser Lys Trp Ser Phe 
625                 630                 635                 640 


Glu Gln Tyr Lys Ala Leu Val Met Asp Leu Ser Lys Lys Lys Glu Tyr 
                645                 650                 655     


Gly Leu Ser Lys Lys Lys Val Arg Asn Leu Leu Tyr Ser Glu Asp Ile 
            660                 665                 670         


Thr Lys Ala Asp Val Leu Arg Gly Phe Ile Asn Arg Asn Ile Asn Asp 
        675                 680                 685             


Thr Ser Tyr Ala Ala Arg Val Val Leu Asn Thr Val Gln Gly Tyr Phe 
    690                 695                 700                 


Arg Ala Lys Glu Met Gly Thr Lys Val Lys Val Ile Arg Gly Ser Tyr 
705                 710                 715                 720 


Thr His Gln Met Arg Val Asn Met Lys Leu Asp Lys Asp Arg Glu Lys 
                725                 730                 735     


Ser Tyr Ala His His Ala Val Asp Ala Met Leu Ile Ala Phe Ser Gln 
            740                 745                 750         


Leu Gly Tyr Glu Ala Tyr Arg Lys Leu Gln Gly Glu Phe Ile Asp Phe 
        755                 760                 765             


Glu Thr Gly Glu Ile Leu Asn Ser Glu Lys Trp Lys Asp Gln Met Ser 
    770                 775                 780                 


Asp Glu Val Tyr Lys Gln Tyr Leu Tyr Arg Lys Lys Trp Ser Asn Ile 
785                 790                 795                 800 


Arg Asn Glu Ile Thr Arg Ala Glu Lys Asn Val Lys Tyr Trp Tyr Tyr 
                805                 810                 815     


Val Asp His Lys Ser Asn Arg Gly Leu Cys Asn Gln Lys Ile Arg Gly 
            820                 825                 830         


Thr Arg Val Tyr Asp Gly Lys Ile Tyr Lys Ile Asn Lys Leu Asp Ile 
        835                 840                 845             


Arg Lys Lys Glu Gly Ala Ala Leu Phe Lys Lys Leu Ala Phe Ser Glu 
    850                 855                 860                 


Lys Glu Ser Asp Arg Glu Lys Leu Leu Val Tyr Lys Asn Asp Lys Lys 
865                 870                 875                 880 


Thr Tyr Glu Asp Leu Val Arg Ile Met Gln Asp Tyr Ser Asp Ser Lys 
                885                 890                 895     


Asn Pro Phe Val Gln Tyr Glu Lys Glu Thr Gly Asp Tyr Val Arg Lys 
            900                 905                 910         


Tyr Ala Lys Lys His Asn Gly Pro Arg Ile Asp Lys Leu Lys Tyr Ile 
        915                 920                 925             


Asp Gly Glu Val Gly Ser Cys Ile Asp Ile Ser His Lys Tyr Gly Tyr 
    930                 935                 940                 


Glu Lys Gly Ser Arg Lys Val Val Leu Glu Ser Leu Val Pro Tyr Arg 
945                 950                 955                 960 


Met Asp Val Tyr Tyr Lys Lys Glu Glu Gln Thr Tyr Tyr Leu Val Gly 
                965                 970                 975     


Ile Lys Gln Ser Asp Val Lys Cys Gln Lys Gly Glu Tyr Val Ile Asp 
            980                 985                 990         


Glu Asp Ala Tyr Ala Ile Asn Leu  Val Lys Glu Lys Met  Ile Gln Gln 
        995                 1000                 1005             


Gly Gln  Lys Arg Ser Asp Leu  Glu Ser Leu Gly Phe  Glu Phe Gln 
    1010                 1015                 1020             


Leu Ser  Phe Tyr Lys Asn Asp  Ile Ile Glu Tyr Glu  Lys Asp Gly 
    1025                 1030                 1035             


Lys Ile  Val Arg Glu Arg Phe  Leu Ser Arg Thr Met  Pro Lys Val 
    1040                 1045                 1050             


Lys Asn  Tyr Ile Glu Thr Lys  Pro Ile Asp Arg Ala  Lys Tyr Glu 
    1055                 1060                 1065             


Trp His  Asp Arg Lys Leu Val  Gly Leu Gly Lys Thr  Lys Arg Ile 
    1070                 1075                 1080             


Arg Lys  Tyr Arg Met Asp Ile  Leu Gly Asn Tyr Tyr  Leu Cys Asn 
    1085                 1090                 1095             


Lys Glu  Glu Phe Ser Lys Tyr  Cys 
    1100                 1105     


<210>  648
<211>  1107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  648

Met Gly Tyr Thr Val Gly Leu Asp Ile Gly Val Ala Ser Val Gly Val 
1               5                   10                  15      


Ala Val Leu Asp Glu Asn Asp Asn Ile Val Glu Ala Val Ser Asn Ile 
            20                  25                  30          


Phe Asp Glu Ala Asp Thr Ser Asn Asn Lys Val Arg Arg Thr Leu Arg 
        35                  40                  45              


Glu Gly Arg Arg Thr Lys Arg Arg Gln Lys Thr Arg Ile Glu Asp Phe 
    50                  55                  60                  


Lys Gln Leu Trp Glu Thr Ser Gly Tyr Ile Ile Pro His Lys Leu His 
65                  70                  75                  80  


Leu Asn Ile Ile Glu Leu Arg Asn Lys Gly Leu Thr Glu Leu Leu Ser 
                85                  90                  95      


Leu Asp Glu Leu Tyr Cys Val Leu Leu Ser Met Leu Lys His Arg Gly 
            100                 105                 110         


Ile Ser Tyr Leu Glu Asp Ala Asp Asp Gly Glu Lys Gly Asn Ala Tyr 
        115                 120                 125             


Lys Lys Gly Leu Ala Phe Asn Glu Lys Gln Leu Lys Glu Lys Met Pro 
    130                 135                 140                 


Cys Glu Ile Gln Leu Glu Arg Met Lys Lys Tyr Gly Lys Tyr His Gly 
145                 150                 155                 160 


Glu Phe Ile Ile Glu Ile Asn Asp Glu Lys Glu Tyr Gln Ser Asn Val 
                165                 170                 175     


Phe Thr Thr Lys Ala Tyr Lys Lys Glu Leu Glu Lys Ile Phe Glu Thr 
            180                 185                 190         


Gln Arg Cys Asn Gly Asn Lys Ile Asn Thr Lys Phe Ile Lys Lys Tyr 
        195                 200                 205             


Met Glu Ile Tyr Glu Arg Lys Arg Glu Tyr Tyr Ile Gly Pro Gly Asn 
    210                 215                 220                 


Glu Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Thr Thr Arg Thr Asp Glu 
225                 230                 235                 240 


Glu Gly Asn Phe Ile Asp Glu Lys Asn Ile Phe Gly Lys Leu Ile Gly 
                245                 250                 255     


Lys Cys Ser Val Tyr Pro Glu Glu Tyr Arg Ala Ser Ser Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Lys Ile 
        275                 280                 285             


Asn Asn Glu Lys Leu Thr Glu Phe Gln Lys Lys Glu Ile Val Glu Ile 
    290                 295                 300                 


Ile Lys Asp Ala Ser Ser Val Asn Met Arg Lys Ile Ile Lys Lys Val 
305                 310                 315                 320 


Ile Asp Glu Asp Ile Glu Gln Tyr Ser Gly Ala Arg Ile Asp Lys Lys 
                325                 330                 335     


Gly Lys Glu Ile Tyr His Thr Phe Glu Ile Tyr Arg Lys Leu Lys Lys 
            340                 345                 350         


Glu Leu Lys Thr Ile Asn Val Asp Ile Asp Ser Phe Thr Arg Glu Glu 
        355                 360                 365             


Leu Asp Lys Thr Met Asp Ile Leu Thr Leu Asn Thr Glu Arg Glu Ser 
    370                 375                 380                 


Ile Val Lys Ala Phe Asp Glu Gln Lys Phe Val Tyr Glu Glu Asn Leu 
385                 390                 395                 400 


Ile Lys Lys Leu Ile Glu Phe Arg Lys Asn Asn Gln Arg Leu Phe Ser 
                405                 410                 415     


Gly Trp His Ser Phe Ser Tyr Lys Ala Met Leu Gln Leu Ile Pro Val 
            420                 425                 430         


Met Tyr Lys Glu Pro Lys Glu Gln Met Gln Leu Leu Thr Glu Met Asn 
        435                 440                 445             


Val Phe Lys Ser Lys Lys Glu Lys Tyr Val Asn Tyr Lys Tyr Ile Pro 
    450                 455                 460                 


Glu Asn Glu Val Val Lys Glu Ile Tyr Asn Pro Val Val Val Lys Ser 
465                 470                 475                 480 


Ile Arg Thr Thr Val Lys Ile Leu Asn Ala Leu Ile Lys Lys Tyr Gly 
                485                 490                 495     


Tyr Pro Glu Ser Val Val Ile Glu Met Pro Arg Asp Lys Asn Ser Asp 
            500                 505                 510         


Asp Glu Lys Glu Lys Ile Asp Met Asn Gln Lys Lys Asn Gln Glu Glu 
        515                 520                 525             


Tyr Glu Lys Ile Leu Asn Lys Ile Tyr Asp Glu Lys Gly Ile Glu Ile 
    530                 535                 540                 


Thr Asn Lys Asp Tyr Lys Lys Gln Lys Lys Leu Val Leu Lys Leu Lys 
545                 550                 555                 560 


Leu Trp Asn Glu Gln Glu Gly Leu Cys Leu Tyr Ser Gly Lys Lys Ile 
                565                 570                 575     


Ala Ile Glu Asp Leu Leu Asn His Pro Glu Phe Phe Glu Ile Asp His 
            580                 585                 590         


Ile Ile Pro Lys Ser Ile Ser Leu Asp Asp Ser Arg Ser Asn Lys Val 
        595                 600                 605             


Leu Val Tyr Lys Thr Glu Asn Ser Ile Lys Glu Asn Asp Thr Pro Tyr 
    610                 615                 620                 


His Tyr Leu Thr Arg Ile Asn Gly Lys Trp Gly Phe Asp Glu Tyr Lys 
625                 630                 635                 640 


Ala Asn Val Leu Glu Leu Arg Arg Arg Gly Lys Ile Asp Asp Lys Lys 
                645                 650                 655     


Val Asn Asn Leu Leu Cys Met Glu Asp Ile Thr Lys Ile Asp Val Val 
            660                 665                 670         


Lys Gly Phe Ile Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ala Ser Arg 
        675                 680                 685             


Val Val Leu Asn Glu Met Gln Ser Phe Phe Glu Ser Arg Lys Tyr Cys 
    690                 695                 700                 


Asn Thr Lys Val Lys Val Ile Arg Gly Ser Leu Thr Tyr Gln Met Arg 
705                 710                 715                 720 


Gln Asp Leu His Leu Lys Lys Asn Arg Glu Glu Ser Tyr Ser His His 
                725                 730                 735     


Ala Val Asp Ala Met Leu Ile Ala Phe Ser Gln Lys Gly Tyr Glu Ala 
            740                 745                 750         


Tyr Arg Lys Ile Gln Lys Asp Cys Tyr Asp Phe Glu Thr Gly Glu Ile 
        755                 760                 765             


Leu Asp Lys Glu Lys Trp Asn Lys Tyr Ile Asp Asp Asp Glu Phe Asp 
    770                 775                 780                 


Asp Ile Leu Tyr Lys Glu Arg Met Asn Glu Ile Arg Lys Lys Ile Ile 
785                 790                 795                 800 


Glu Ala Glu Glu Lys Val Lys Tyr Asn Tyr Lys Ile Asp Lys Lys Cys 
                805                 810                 815     


Asn Arg Gly Leu Cys Asn Gln Thr Ile Tyr Gly Thr Arg Glu Lys Asp 
            820                 825                 830         


Gly Lys Ile His Lys Ile Ser Ser Tyr Asn Ile Tyr Asp Asp Lys Glu 
        835                 840                 845             


Cys Asn Ser Leu Lys Lys Met Ile Asn Ser Gly Lys Gly Ser Asp Leu 
    850                 855                 860                 


Leu Met Tyr Asn Asn Asp Pro Lys Thr Tyr Arg Asp Met Leu Lys Ile 
865                 870                 875                 880 


Leu Glu Thr Tyr Ser Ser Glu Lys Asn Pro Phe Val Ala Tyr Asn Lys 
                885                 890                 895     


Glu Thr Gly Asp Tyr Phe Arg Lys Tyr Ser Lys Asn His Asn Gly Pro 
            900                 905                 910         


Lys Val Glu Lys Val Lys Tyr Tyr Ser Gly Gln Ile Asn Ser Cys Ile 
        915                 920                 925             


Asp Ile Ser His Lys Tyr Gly His Ala Lys Asn Ser Lys Lys Val Val 
    930                 935                 940                 


Leu Val Ser Leu Asn Pro Tyr Arg Thr Asp Val Tyr Tyr Asp Asn Asp 
945                 950                 955                 960 


Thr Gly Lys Tyr Tyr Leu Val Gly Val Lys Tyr Asn His Ile Lys Cys 
                965                 970                 975     


Val Gly Asn Lys Tyr Val Ile Asp Ser Glu Thr Tyr Asn Glu Leu Leu 
            980                 985                 990         


Arg Lys Glu Gly Val Leu Asn Ser  Asp Glu Asn Leu Glu  Asp Leu Asn 
        995                 1000                 1005             


Ser Lys  Asn Ile Thr Tyr Lys  Phe Ser Leu Tyr Lys  Asn Asp Ile 
    1010                 1015                 1020             


Ile Gln  Tyr Glu Lys Gly Gly  Glu Tyr Tyr Thr Glu  Arg Phe Leu 
    1025                 1030                 1035             


Ser Arg  Ile Lys Glu Gln Lys  Asn Leu Ile Glu Thr  Lys Pro Ile 
    1040                 1045                 1050             


Asn Lys  Pro Asn Phe Gln Arg  Lys Asn Lys Lys Gly  Glu Trp Glu 
    1055                 1060                 1065             


Asn Thr  Arg Asn Gln Ile Ala  Leu Ala Lys Thr Lys  Tyr Val Gly 
    1070                 1075                 1080             


Lys Leu  Val Thr Asp Val Leu  Gly Asn Cys Tyr Ile  Val Asn Met 
    1085                 1090                 1095             


Glu Lys  Phe Ser Leu Val Val  Asp Lys 
    1100                 1105         


<210>  649
<211>  1107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  649

Met Gly Tyr Thr Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Phe 
1               5                   10                  15      


Gly Leu Ile Asp Asn Val Asp Gly Lys Ile Leu Glu Ser Gly Val Arg 
            20                  25                  30          


Leu Phe Ser Ser Ala Asp Pro Asp Asn Asn Gln Thr Arg Arg Ser Ser 
        35                  40                  45              


Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Val His Arg Leu Glu Arg 
    50                  55                  60                  


Val Ala His Leu Leu Glu Glu Ala Gly Phe Glu Lys Gly Glu Val Leu 
65                  70                  75                  80  


Ala Pro Thr Pro Tyr His Leu Arg Val Lys Gly Leu Thr Glu Lys Leu 
                85                  90                  95      


Ser Lys Pro Glu Val Tyr Ala Ala Leu Tyr Asn Leu Met Lys His Arg 
            100                 105                 110         


Gly Ile Ser Tyr Leu Glu Asp Val Glu Leu Asp Glu Ala Asp Ser Ser 
        115                 120                 125             


Ser Val Ser Gln Asn Val Glu Leu Leu Lys Glu Tyr Tyr Pro Cys Glu 
    130                 135                 140                 


Ile Gln Leu Glu Arg Tyr Glu Glu Tyr Gly Gln Ile Arg Gly Ile Ile 
145                 150                 155                 160 


Glu Thr Tyr Asn Lys Asp Gly Glu Lys Glu Ser Leu Val Asn Ile Phe 
                165                 170                 175     


Pro Thr Gly Ala Tyr Lys Lys Glu Ala Glu Ala Ile Ile Leu Lys Gln 
            180                 185                 190         


Gly Gln Phe Tyr Pro Glu Leu Asp Lys Val Phe Leu Glu Lys Phe Thr 
        195                 200                 205             


Glu Ile Leu Thr Val Lys Arg Pro Tyr Tyr Val Gly Pro Gly Asn Lys 
    210                 215                 220                 


Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Arg Glu Asn Gly Glu Thr Leu 
225                 230                 235                 240 


Glu Asn Leu Phe Asp Ile Leu Ile Gly Lys Cys Thr Ile Tyr Pro Gln 
                245                 250                 255     


Glu Ala Arg Ala Ala Lys Phe Ser Tyr Thr Ala Gln Leu Phe Asp Phe 
            260                 265                 270         


Leu Asn Asp Met Asn Asn Ile Ser Ile Ala Gly Glu Lys Leu Thr Glu 
        275                 280                 285             


Val Gln Lys Arg Glu Ile Tyr His Gln Ile Met Ser Ser Lys Thr Val 
    290                 295                 300                 


Asn Met Met Lys Ile Ile Thr Lys Val Thr Gly Cys Leu Lys Glu Glu 
305                 310                 315                 320 


Val Thr Gly Tyr Arg Val Asp Ser Lys Tyr Lys Ala Glu Phe His Ser 
                325                 330                 335     


Phe Ala Ser Tyr Arg Gln Phe Arg Asn Lys Met Leu Asp Tyr Gly Phe 
            340                 345                 350         


Asp Val Gln Thr Leu Asn Val Glu Met Phe Asp Lys Leu Ala Ala Lys 
        355                 360                 365             


Leu Thr Leu Asn Thr Glu Tyr His Gln Ile Ile Thr Gln Leu Glu Ser 
    370                 375                 380                 


Leu Asn Leu Gly Leu Ser Lys Glu Leu Ile Glu Lys Ile Ala Asp Phe 
385                 390                 395                 400 


Arg Lys Lys Asn Arg Ser Leu Phe Ser Lys Trp His Ser Leu Ser Leu 
                405                 410                 415     


Lys Ala Met Asn Glu Val Val Asp Glu Leu Trp Ser Thr Ser Lys Asn 
            420                 425                 430         


Gln Met Gln Ile Phe Ser Glu Leu Gly Gly Phe Lys Ser Asn Ile Glu 
        435                 440                 445             


Val Phe Lys Gly Leu Lys Tyr Ile Pro Leu Asp Ala Met Val Asp Glu 
    450                 455                 460                 


Ile Tyr Asn Pro Val Val Arg Cys Ser Ile Arg Glu Ser Ile Lys Ile 
465                 470                 475                 480 


Ile Asn Ala Leu Leu Lys Lys Tyr Gly Ala Leu Asp Lys Val Val Ile 
                485                 490                 495     


Glu Met Pro Arg Asp Ser Asn Glu Lys Glu Glu Lys Asp Arg Ile Thr 
            500                 505                 510         


Lys Leu Asn Lys Glu Asn Asp Asn Lys Lys Lys Gln Ala Ile Glu Lys 
        515                 520                 525             


Ala Arg Asn Glu Tyr Gly Phe Gly Glu Glu Ala Tyr Arg Gln Gln Asn 
    530                 535                 540                 


Asp Leu Asn Thr Lys Leu Arg Leu Trp Tyr Glu Gln Lys Gln Arg Cys 
545                 550                 555                 560 


Leu Tyr Ser Gly Glu Ile Ile Lys Ile Phe Asp Leu Ile Lys Asn Pro 
                565                 570                 575     


His Leu Phe Asp Ile Asp His Ile Ile Pro Lys Ser Ile Ser Tyr Asp 
            580                 585                 590         


Asp Ser Leu Ser Asn Lys Val Leu Cys Tyr Ala Thr Glu Asn Arg Glu 
        595                 600                 605             


Lys Gly Asn Leu Thr Pro Tyr Arg Tyr Phe Lys Arg Lys Ala Ser Leu 
    610                 615                 620                 


Thr Trp Asn Tyr Glu Ala Tyr Lys Ser Tyr Val Ile Glu Leu Tyr Lys 
625                 630                 635                 640 


Asp Gly Lys Gly Asp Leu Ser Arg Thr Lys Leu Glu Leu Leu Leu Phe 
                645                 650                 655     


Glu Glu Asp Ile Asn Lys Tyr Glu Val Arg Gln Arg Phe Ile Asn Arg 
            660                 665                 670         


Asn Leu Val Asp Thr Arg Tyr Ala Ser Lys Val Val Leu Asn Thr Leu 
        675                 680                 685             


Gln Asp Phe Met Lys Glu Asn Tyr Pro Thr Thr Thr Val Glu Val Val 
    690                 695                 700                 


Arg Gly Lys Phe Thr Ala Gln Leu Arg Arg His Trp Gly Ile Arg Lys 
705                 710                 715                 720 


Asn Arg Asp Glu Asn His Ala His His Ala Ile Asp Ala Leu Ile Ile 
                725                 730                 735     


Ala Cys Val Pro His Leu Ser Leu Trp Lys Glu Gln Lys Lys Val Phe 
            740                 745                 750         


Ser Thr Asp Glu Phe Arg Val Asp Glu Thr Thr Gly Glu Ile Ile Glu 
        755                 760                 765             


Gln Ser Tyr Asn Gln Leu Thr Tyr Gln Glu Pro Phe Ser Gln Phe Phe 
    770                 775                 780                 


Tyr Gln Leu Glu Lys Val Ser Ile Asp Cys Arg Tyr Ser Tyr Lys Val 
785                 790                 795                 800 


Asp Arg Lys Phe Asn Arg Gln Val Ala Asp Ala Thr Ile Tyr Ser Thr 
                805                 810                 815     


Arg Gln Gly Ile Val Lys Gln Thr Ser Gly Asp Lys Ser Phe Thr Leu 
            820                 825                 830         


Asp Ser Asn Ser Lys Glu His Tyr Val Ile Ala Lys Ile Lys Asn Ile 
        835                 840                 845             


Tyr Glu Asp Val Asp Ala Lys Arg Phe Met Asp Arg Tyr Lys Lys Asp 
    850                 855                 860                 


Lys Thr Gln Phe Leu Met Tyr His His Asp Pro Gln Thr Phe Ser Ile 
865                 870                 875                 880 


Leu Glu Asp Val Met Lys Thr Tyr Pro Asp Lys Lys Asn Pro Phe Gln 
                885                 890                 895     


Ala Tyr Arg Leu Glu Tyr Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly 
            900                 905                 910         


Asn Gly Pro Gln Ile Gln Ser Ile Lys Tyr Tyr Asp Ser Lys Leu Gly 
        915                 920                 925             


Gln His Ile Glu Leu Lys Gln Ala Asn Gln Ser Thr Lys Lys Val Val 
    930                 935                 940                 


Leu Gln Ser Leu Asn Pro Trp Arg Cys Asp Ile Tyr Tyr Asn Lys Lys 
945                 950                 955                 960 


Asp Lys Arg Tyr Ile Ala Ala Gly Leu Lys Tyr Ala Asp Leu Ser Tyr 
                965                 970                 975     


Gln Lys Gly Thr Gly Leu Tyr Gly Ile Ser Leu Gln Arg Tyr Gln Gln 
            980                 985                 990         


Ile Leu Asn Ser Glu Lys Ile Glu  Leu Ser Ile Glu Gln  Ile Glu Leu 
        995                 1000                 1005             


Leu Leu  Glu Gly Arg Leu Asn  Glu Ser Asn Phe Glu  Phe Cys Phe 
    1010                 1015                 1020             


Ser Leu  Tyr Lys Asn Asp Phe  Ile Gln Trp Asn Asp  Glu Asn Asn 
    1025                 1030                 1035             


Gln Thr  Phe Gln Tyr Arg Phe  Leu Ala Arg Asn Leu  Ser Ser Pro 
    1040                 1045                 1050             


Asn Arg  Ile Glu Val Lys Pro  Ile Asn Lys Ser Lys  Tyr Asp Lys 
    1055                 1060                 1065             


Gln Ala  Glu Gly Leu Lys Thr  Leu Lys Lys Gly Leu  Asn Arg Phe 
    1070                 1075                 1080             


Tyr Lys  Ile Ser Val Asp Val  Leu Gly Tyr Gln His  Phe Ile Lys 
    1085                 1090                 1095             


Lys Glu  Lys Leu Lys Leu Thr  Phe Lys 
    1100                 1105         


<210>  650
<211>  1109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  650

Met Ser His Tyr Val Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly 
1               5                   10                  15      


Tyr Gly Ile Ile Asp Leu Asp Thr Asn Asp Phe Ile Asp Tyr Gly Val 
            20                  25                  30          


Arg Leu Phe Lys Glu Gly Thr Ala Ala Asn Asn Glu Asp Arg Arg Asn 
        35                  40                  45              


Ser Arg Ser Arg Arg Arg Leu Leu Ser Arg Lys His Thr Arg Leu Met 
    50                  55                  60                  


Asp Met Gln Lys Val Leu Lys Glu Ala Gly Ile Met Thr Glu Ser Tyr 
65                  70                  75                  80  


Ser Pro Leu Ser Asn Val Tyr Glu Ile Arg Lys Lys Gly Leu Thr Lys 
                85                  90                  95      


Glu Leu Thr Asn Asp Glu Leu Thr Ala Ala Ile Leu His Ile Thr Lys 
            100                 105                 110         


His Arg Gly Ser Val Ile Glu Thr Val Glu Asp Asp Glu Ala Ala Ala 
        115                 120                 125             


Lys Glu Thr Gly Glu Leu Lys Ser Thr Leu His Glu Asn Ala Leu Leu 
    130                 135                 140                 


Leu Ala Asn Asn Lys Phe Val Cys Glu Ile Gln Leu Glu Arg Leu Asn 
145                 150                 155                 160 


Thr Glu Asn Lys Ile Arg Asn His Lys Asn Asn Phe Lys Thr Lys Asp 
                165                 170                 175     


Tyr Val Ala Glu Leu Lys Glu Ile Leu Asn His Gln Asn Leu Ser Glu 
            180                 185                 190         


Glu Val Lys Asn Lys Ile Ile Gln Ile Val Glu Arg Arg Arg Ala Tyr 
        195                 200                 205             


Tyr Glu Gly Pro Gly Ser Phe Asn Ser Pro Thr Pro Tyr Gly Arg Phe 
    210                 215                 220                 


Ile Glu Val Asn Gly Gln Val Gln Glu Ile Asp Leu Ile Glu Lys Met 
225                 230                 235                 240 


Arg Gly Lys Cys Ser Val Tyr Pro Asp Glu Phe Arg Ala Pro Lys Met 
                245                 250                 255     


Ser Ile Ser Ala Asp Leu Phe Asn Ile Leu Asn Asp Phe Asn Asn Leu 
            260                 265                 270         


Leu Val Gln Gly Glu Lys Leu Gln Glu Glu Asp Lys Lys Asn Leu Leu 
        275                 280                 285             


Lys Ile Ile Asn Asp Lys Gly Ser Ile Thr Leu Arg Gln Ile Ser Lys 
    290                 295                 300                 


Lys Leu Glu Val Asn Glu Asp Glu Ile Lys Gly Tyr Arg Ile Asp Lys 
305                 310                 315                 320 


Lys Gly Lys Ala Leu Phe Thr Glu Phe Lys Gly Tyr Lys Ile Leu Lys 
                325                 330                 335     


Lys Leu Phe Leu Asn Cys Gly Gln Ser Ile Ser Leu Glu Asp Tyr Glu 
            340                 345                 350         


Met Leu Asp Glu Ile Ile Glu Ile Val Thr Lys Lys Lys Gly Ile Asp 
        355                 360                 365             


Glu Arg Lys Asp Ala Leu Lys Glu Leu Lys Trp Asp Leu Ser Asp Asp 
    370                 375                 380                 


Ile Ile Asn Ser Leu Ser Glu Val Thr Gly Ile Val Gly Tyr His Ser 
385                 390                 395                 400 


Leu Ser Leu Lys Ala Ile Arg Glu Leu Asp Ser Glu Leu Tyr Lys Thr 
                405                 410                 415     


Ser Leu Asn Gln Met Gln Leu Leu His Glu Leu Lys Met Phe Asp Lys 
            420                 425                 430         


Asn Arg Pro Ser Tyr Lys Gly Lys Lys Asn Ile Val Ala Asp Pro Glu 
        435                 440                 445             


Ala Ile Leu Ser Pro Val Ala Lys Arg Ala Gln Asn Glu Thr Phe Lys 
    450                 455                 460                 


Val Ile Asn Ala Leu Arg Lys Lys Tyr Gly Glu Phe Asp Ser Ile Val 
465                 470                 475                 480 


Val Glu Met Thr Arg Asp Lys Asn Ser Gln Glu Gln Lys Lys Arg Ile 
                485                 490                 495     


Asn Asp Ser Gln Lys Ala Leu Glu Ser Leu Asn Lys Glu Val Asp Gln 
            500                 505                 510         


Leu Leu Gln Gln Lys Gly Tyr Asp Pro Gln Lys Val Lys Gly Lys Thr 
        515                 520                 525             


Lys Gln Lys Val Arg Leu Tyr Met Met Gln Glu Gly Lys Ser Ala Tyr 
    530                 535                 540                 


Thr Leu Gln Glu Leu Asp Leu His Arg Val Ile Lys Asp Asp Lys Tyr 
545                 550                 555                 560 


Thr Glu Ile Asp His Ile Ile Pro Ile Ser Ile Ser Leu Asp Asp Ser 
                565                 570                 575     


Gln Ser Asn Lys Val Leu Ala Leu His Ser Glu Asn Gln Gln Lys Gly 
            580                 585                 590         


Asn Leu Thr Pro Phe Met Ala Phe His Thr Gly Arg Phe Ala Asp Met 
        595                 600                 605             


Gly Cys Thr Trp Asn Lys Phe Lys Glu Asn Val Leu Ser Asn Lys Asn 
    610                 615                 620                 


Tyr Lys His Lys Lys Lys Asn Asn Leu Leu Asp Glu Arg Asp Ile Asn 
625                 630                 635                 640 


Lys Phe Ser Val Ile Gln Glu Phe Ile Asn Arg Asn Leu Val Asp Thr 
                645                 650                 655     


Ser Tyr Ala Asn Arg Val Val Leu Asn Thr Leu Thr Asp Tyr Phe Lys 
            660                 665                 670         


Asp Asn Glu Ile Asp Thr Lys Val His Thr Val Asn Gly Lys Leu Thr 
        675                 680                 685             


Asp Leu Phe Arg Lys Arg Ile Asn Leu Lys Lys Asp Arg Asn Glu Asp 
    690                 695                 700                 


Tyr Leu His His Ala Ile Asp Ala Leu Ile Val Ala Ser Ile Lys Lys 
705                 710                 715                 720 


Leu Arg Leu Leu Asp Gly Tyr Leu Ala Lys Tyr Gly Phe Glu Thr Leu 
                725                 730                 735     


Tyr Asp Glu Met Thr Gly Glu Ile Lys Glu Ile Pro Glu Asp Lys Ala 
            740                 745                 750         


Phe Phe Asp Asp Lys Tyr Ile Arg Tyr Ile Leu Asn Leu Lys Thr Ile 
        755                 760                 765             


Tyr Glu Ser Ser Asn Met Tyr Tyr Arg Gly Ile Leu Glu Arg Glu Glu 
    770                 775                 780                 


Leu Ile Tyr Gln Pro Ile Lys Ile Ser His Lys Ile Asp Thr Lys Pro 
785                 790                 795                 800 


Asn Arg Lys Val Ser Asp Glu Thr Ile Tyr Ser Thr Arg Ile Ile Asp 
                805                 810                 815     


Asn Glu Asp His Leu Ile Glu Lys Tyr Ser Asp Ile Tyr Asp Pro Lys 
            820                 825                 830         


Phe Lys Asn Leu Thr Glu Asp Ile Leu Asn Gly Glu Lys Asn Lys Tyr 
        835                 840                 845             


Leu Met Glu Lys Asn Asp Pro Gln Thr Phe Asn Ile Leu Lys Gln Ile 
    850                 855                 860                 


Ile Phe Asn His Tyr Asn Thr Tyr Lys Asp Ser Lys Glu Tyr Tyr Lys 
865                 870                 875                 880 


Ile Glu Lys Lys Lys Glu Lys Glu Ile Ile Lys Leu Val Gly Ile Asn 
                885                 890                 895     


Pro Leu Ser Leu Tyr Lys Glu Glu Asn Gly Pro Ile Lys Lys Tyr Ser 
            900                 905                 910         


Lys Lys Gly Asn Gly Pro Val Ile Met Ser Met Lys Tyr Asp Ser Glu 
        915                 920                 925             


Lys Leu Gly Asn His Ile Asp Ile Thr Pro Asp Ser Ala Lys Asn Lys 
    930                 935                 940                 


Lys Val Ile Leu Lys Gln Ile Ser Pro Tyr Arg Thr Asp Phe Tyr Lys 
945                 950                 955                 960 


Cys Ser Asp Glu Lys Phe Arg Phe Val Thr Val Arg Tyr Lys Asp Val 
                965                 970                 975     


His Tyr Gln Lys Ser Ile Asp Lys Tyr Cys Ile Asp Lys Glu Trp Tyr 
            980                 985                 990         


Gln Asn Glu Lys Lys Asn Lys Lys  Ile Asp Glu Lys Ala  Glu Phe Leu 
        995                 1000                 1005             


Phe Ser  Met His His Asp Glu  Leu Ile Gly Ile Thr  Arg Lys Glu 
    1010                 1015                 1020             


Ser Glu  Gln Phe Ile Tyr Asp  Glu Ser Lys Glu Asn  His Gly Asp 
    1025                 1030                 1035             


Pro Arg  Tyr His Asp Gly Val  Lys Pro Glu Ile Leu  Lys Phe Thr 
    1040                 1045                 1050             


Ala Thr  Asn Asn Asp Thr Lys  Gly Ile Ile Glu Val  Lys Pro Leu 
    1055                 1060                 1065             


Tyr Thr  Tyr Ser Ala Tyr Arg  Leu Lys Pro Thr Val  Leu Asn Val 
    1070                 1075                 1080             


Lys Asn  Ile Lys Lys Tyr Ala  Thr Asp Val Leu Gly  Asn Leu Tyr 
    1085                 1090                 1095             


Glu Val  Lys Gln Asn Glu Leu  Lys Leu Glu Phe 
    1100                 1105                 


<210>  651
<211>  1110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  651

Met Ala Glu Gly Lys Lys Arg Ile Trp Arg Leu Gly Ala Asp Leu Gly 
1               5                   10                  15      


Ala Asn Ser Leu Gly Trp Ala Ala Ile Glu Leu Ser Ala Pro Val Asn 
            20                  25                  30          


Gly Lys Pro Ile Ala Ile Leu Ala Ala Gly Ser Arg Ile Phe Ser Asp 
        35                  40                  45              


Gly Arg Asp Pro Lys Ser Gly Ala Ser Leu Ala Val Asp Arg Arg Asp 
    50                  55                  60                  


Ala Arg Ala Met Arg Arg Arg Arg Asp Arg Phe Lys Gln Arg Gln Lys 
65                  70                  75                  80  


Ala Leu Ile Lys Tyr Leu Ile Ala Asp Gly Leu Phe Pro Ala Asp Glu 
                85                  90                  95      


Ala Ala Arg Asn Ala Met Ala Ala Ile Asp Pro Tyr Ala Val Arg Ala 
            100                 105                 110         


Arg Ala Leu Asp Glu Ala Leu Ser Leu Pro Glu Leu Gly Arg Ala Leu 
        115                 120                 125             


Phe His Leu Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Ala Asp 
    130                 135                 140                 


Arg Lys Ala Asp Asp Asp Ala Gly Lys Ile Ala Ile Gly Ile Asp Arg 
145                 150                 155                 160 


Thr Arg Asp Ala Ile Ala Glu Asp Glu Ala Asp Ala Gly Ala Glu Pro 
                165                 170                 175     


Gly Ser Trp Thr Phe Gly Lys Trp Leu His Glu Arg Arg Leu Lys Gly 
            180                 185                 190         


Gln Ser Val Arg Thr Arg Leu Arg Pro Glu Ser Gly Glu Asp Ala Lys 
        195                 200                 205             


Gly Asp Gly Tyr Asp Phe Tyr Pro Asp Arg Ala Leu Met Glu Glu Glu 
    210                 215                 220                 


Phe Asp Ala Ile Trp Ala Ala Gln Ala Pro His His Pro Asp Val Leu 
225                 230                 235                 240 


Thr Asn Asp Val His Asp Arg Leu His Glu Ile Ile Phe Tyr Gln Arg 
                245                 250                 255     


Pro Leu Lys Ala Pro Gln Val Gly Met Cys Ser Leu Ile Pro Gly Asp 
            260                 265                 270         


Arg Arg Leu Pro Lys Ala His Pro Leu Phe Gln Arg Arg Arg Leu Leu 
        275                 280                 285             


Glu Glu Leu Asn Ala Leu Met Ile Val Arg Pro Gly Glu Val Ala Gln 
    290                 295                 300                 


Arg Leu Thr Arg Glu Glu Arg Asp Ile Leu Leu Leu Lys Leu Lys Asp 
305                 310                 315                 320 


Lys Asn Lys Val Ser Phe Ala Thr Leu Arg Lys Ala Leu Lys Leu Asp 
                325                 330                 335     


Pro Asp Ala Arg Phe Asn Lys Glu Ser Glu Asp Arg Thr Asp Leu Lys 
            340                 345                 350         


Gly Asp Glu Val Ala Ala Val Met Gly Ser Lys Thr Arg Phe Gly Asn 
        355                 360                 365             


Leu Trp Val His Leu Ser Ile Glu Ala Gln Trp Asp Val Leu Ala Arg 
    370                 375                 380                 


Leu Asp Ala Val Glu Ser Asp Ala Glu Asp Ala Ala Phe Cys Ala Trp 
385                 390                 395                 400 


Leu Val Glu Thr Tyr Ala Leu Thr Pro Glu Gln Ala Arg Ala Val Ala 
                405                 410                 415     


Asn Ala Pro Leu Pro Pro Gly Tyr Gly Arg Phe Gly Glu Thr Ala Thr 
            420                 425                 430         


Arg Lys Leu Ile Glu Ala Leu Arg Asp Gly Lys Lys Asp Asp Arg Val 
        435                 440                 445             


Leu Val Tyr Ser Glu Ala Val Thr Glu Ala Gly Leu Gly His His Ser 
    450                 455                 460                 


Asp Arg Arg Thr Gly Glu Val Phe Glu Asp Ala Lys Gly Arg Pro Gln 
465                 470                 475                 480 


Leu Pro Tyr Tyr Gly Val Ala Leu Glu Arg His Ile Met Pro Gly Thr 
                485                 490                 495     


Gly Asp Pro Ser Asp Pro Glu Glu Met Arg Val Gly Arg Leu Thr Asn 
            500                 505                 510         


Pro Thr Val His Ile Gly Leu Asn Gln Leu Arg Arg Ile Leu Asn Val 
        515                 520                 525             


Leu Ile Arg Ala Tyr Gly Pro Pro Ala Glu Ile Ala Ile Glu Leu Ala 
    530                 535                 540                 


Arg Glu Leu Lys Leu Thr Asp Glu Asp Lys Lys Arg Ile Asn Arg Glu 
545                 550                 555                 560 


Asn Asn Asp Asn Arg Ile Ala Ala Glu Arg Arg Ser Glu Lys Leu Leu 
                565                 570                 575     


Ala Ile Gly Gln Ala Asp Ser Gly Ala Asn Arg Ala Leu Leu Lys Leu 
            580                 585                 590         


Trp Glu Glu Leu Asn Pro Ala Asn Val Leu Asp Arg Arg Cys Ile Tyr 
        595                 600                 605             


Ser Gly Arg Gln Ile Ser Ile Asp Met Leu Phe Ser Ala Ala Val Glu 
    610                 615                 620                 


Val Asp His Ile Leu Pro Phe Ala Ala Thr Leu Asp Asp Ser Asn Gly 
625                 630                 635                 640 


Asn Lys Ile Val Cys Leu Arg Glu Ala Asn Arg Glu Lys Arg Lys Arg 
                645                 650                 655     


Ser Pro His Asp Ala Trp Gly His Ser Ala Arg Trp Asp Glu Ile Ala 
            660                 665                 670         


Ala Arg Ala Ala Gln Leu Pro Arg Asn Lys Arg Trp Arg Phe Glu Pro 
        675                 680                 685             


Asp Ala Met Asp Lys Phe Arg Asp Glu Gly Gly Phe Gln Ala Arg His 
    690                 695                 700                 


Leu Val Asp Thr Gln Tyr Leu Ala Arg Leu Ser Arg Glu Tyr Leu Ala 
705                 710                 715                 720 


Thr Leu Tyr Pro Glu Lys Gly Glu Gly Ser Gly His Ile Trp Val Ser 
                725                 730                 735     


Pro Gly Arg Leu Thr Glu Met Val Arg Arg Lys Leu Gly Leu Asn Asp 
            740                 745                 750         


Leu Leu Pro Asp His Asn Phe Gly Gly Gly Ala Asp Gln Ala Lys Asn 
        755                 760                 765             


Arg Leu Asp His Arg His His Ala Ile Asp Ala Val Val Val Ala Ile 
    770                 775                 780                 


Val Asp Arg Gly Met Leu Asn Ala Ile Ala Arg Ala Ser Gly Gln Glu 
785                 790                 795                 800 


Gly Ala Glu Gly Arg Glu Arg Ile Ile Ile Pro Glu Pro Trp Asp Gly 
                805                 810                 815     


Phe Arg Asp Ala Leu Arg Glu Thr Val Asn Arg Ile Thr Val Ser His 
            820                 825                 830         


Arg Thr Asp His Gly Thr Ala Ser Lys Ala Gly Leu Pro Lys Gly Lys 
        835                 840                 845             


Asp Ala Thr Ala Gly Arg Leu His Asn Asp Thr Ala Tyr Gly Phe Thr 
    850                 855                 860                 


Gly Asp Thr Asp Ala Lys Gly Asn Ser Ile Val Val Arg Arg Val Pro 
865                 870                 875                 880 


Leu Ser Ser Phe Lys Lys Leu Ala Asp Leu Asp Arg Ile Arg Asp Ala 
                885                 890                 895     


Asp Leu Lys Ala Ala Leu Tyr Gln His Leu Asn Gly Leu Glu Gly Lys 
            900                 905                 910         


Ala Phe Glu Lys Ala Leu Ala Glu Phe His Lys Leu Glu Trp His Asn 
        915                 920                 925             


Phe Gly Gly Val Arg Arg Val Arg Val Thr Glu Pro Leu Thr Val Ile 
    930                 935                 940                 


Pro Ile Lys Asp Arg Asn Gly Arg Ile Tyr Lys Gly Tyr Lys Gly Asp 
945                 950                 955                 960 


Ser Asn Tyr Arg Tyr Asp Val Trp Glu Leu Pro Asp Gly Lys Trp Val 
                965                 970                 975     


Thr Asn Trp Lys Asn Glu Ala Gly Glu Ala Met Ser Ser Val Ile Ser 
            980                 985                 990         


Met Phe Asp Ala His Gln Ser Ser  Glu Pro Pro Arg Pro  His Pro Ala 
        995                 1000                 1005             


Ala Arg  Lys Ile Leu Ser Leu  His Gln Asn Asp Leu  Leu Ala Ile 
    1010                 1015                 1020             


Glu Arg  Glu Gly Glu Asp Arg  Lys Leu Met Arg Val  Val Lys Phe 
    1025                 1030                 1035             


Gly Gln  Ser Gly Gln Ile Thr  Leu Ala Glu His Gln  Gln Ala Gly 
    1040                 1045                 1050             


Asp Leu  Lys Lys Arg Asp Ala  Val Ser Asn Asp Asp  Asp Pro Phe 
    1055                 1060                 1065             


Lys Tyr  Phe Ala Pro Thr Ser  Gly Gly Leu Lys Lys  Val Lys Ala 
    1070                 1075                 1080             


Arg Gln  Val Arg Ile Asp Glu  Ile Gly Arg Val Leu  Asp Pro Gly 
    1085                 1090                 1095             


Phe Pro  Ala Arg Thr Ala Arg  Arg Lys Thr Arg Gly  
    1100                 1105                 1110 


<210>  652
<211>  1110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  652

Met Thr Ala Arg Thr His Lys Ile Trp Arg Leu Gly Ala Asp Leu Gly 
1               5                   10                  15      


Ala Asn Ser Leu Gly Trp Ala Ala Ile Glu Leu Ser Ala Pro Val Asp 
            20                  25                  30          


Gly Lys Pro Ile Ala Ile Leu His Ala Gly Ser Arg Ile Phe Ser Asp 
        35                  40                  45              


Gly Arg Asp Pro Lys Ser Gly Ala Ser Leu Ala Val Asp Arg Arg Asp 
    50                  55                  60                  


Ala Arg Ala Met Arg Arg Arg Arg Asp Arg Leu Gln Gln Arg Gln Arg 
65                  70                  75                  80  


Ala Leu Ile Lys Tyr Leu Ile Ala Asp Gly Leu Phe Pro Ala Asp Glu 
                85                  90                  95      


Asn Glu Arg Lys Ala Met Ala Thr Ile Asp Pro Tyr Ala Val Arg Ala 
            100                 105                 110         


Arg Ala Leu Asp Glu Ala Leu Thr Leu Pro Glu Val Gly Arg Ala Leu 
        115                 120                 125             


Phe His Leu Asn Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Ala Asp 
    130                 135                 140                 


Arg Lys Ala Asp Glu Asp Ala Gly Lys Ile Ala Ile Gly Ile Asp Arg 
145                 150                 155                 160 


Thr Arg Asp Ala Ile Arg Gln Ala Glu Glu Glu Ala Gly Ala Glu Pro 
                165                 170                 175     


Gly Ser Trp Thr Tyr Gly Lys Trp Leu Tyr Glu Arg Arg Ala Ser Ala 
            180                 185                 190         


Thr Asp Ala Asn Ala Ile Pro Ser Val Arg Thr Arg Leu Arg Pro Glu 
        195                 200                 205             


Thr Asp Glu His Ala Lys Gly Asp Gly Tyr Asp Phe Tyr Pro Gly Arg 
    210                 215                 220                 


Ala Leu Met Glu Ala Glu Phe Ala Ala Ile Trp Glu Ala Gln Ala Ala 
225                 230                 235                 240 


His His Pro Asp Ala Leu Thr Asn Glu Val His Asp Arg Leu His Glu 
                245                 250                 255     


Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ala Pro Gln Ile Gly Val Cys 
            260                 265                 270         


Thr Leu Ile Arg Gly Asp Gln Arg Leu Pro Lys Ala His Pro Leu Phe 
        275                 280                 285             


Gln Lys Arg Arg Leu Leu Glu Glu Leu Asn Ala Leu Thr Ile Val Arg 
    290                 295                 300                 


Pro Gly Glu Met Ala Gln Arg Leu Thr Arg Glu Glu Arg Asp Thr Leu 
305                 310                 315                 320 


Phe Leu Lys Leu Lys Asp Lys Ala Lys Val Ser Phe Val Ser Leu Arg 
                325                 330                 335     


Lys Thr Leu Lys Leu Asp Pro Asp Ala Arg Phe Asn Lys Glu Ser Glu 
            340                 345                 350         


Asn Arg Ser Asp Leu Lys Gly Asp Glu Val Ala Ala Ala Met Gly Ala 
        355                 360                 365             


Lys Thr Arg Phe Gly Asn Arg Trp Val His Leu Ser Ile Glu Glu Gln 
    370                 375                 380                 


Trp Asp Val Ile Ala Arg Leu Glu Glu Val Glu Ser Asp Ala Glu Asp 
385                 390                 395                 400 


Ala Ala Cys Arg Ala Trp Leu Ala Ala Thr Phe Asp Leu Thr Pro Glu 
                405                 410                 415     


Gln Ile Arg Ala Val Thr Asn Ala Pro Leu Pro Pro Gly Tyr Ser Arg 
            420                 425                 430         


Phe Gly Lys Val Ala Thr Gln Arg Leu Ile Asp Ala Leu Arg Asp Gly 
        435                 440                 445             


Glu Lys Asp Gly Arg Val Leu Val Tyr Ser Glu Ala Val Ala Ala Ala 
    450                 455                 460                 


Gly Leu Gly His His Ser Asp Leu Arg Thr Gly Glu Ile Phe Glu Asp 
465                 470                 475                 480 


Ala Gln Gly Arg Pro Gln Leu Pro Tyr Tyr Gly Val Ala Leu Glu Arg 
                485                 490                 495     


His Ile Met Pro Gly Thr Gly Asn Pro Asp His Ser Asp Glu Met Arg 
            500                 505                 510         


Ile Gly Arg Leu Thr Asn Pro Thr Val His Ile Gly Leu Asn Gln Leu 
        515                 520                 525             


Arg Arg Ile Val Asn Thr Leu Ile Arg Ala Tyr Gly Pro Pro Ala Glu 
    530                 535                 540                 


Ile Ala Ile Glu Leu Ala Arg Glu Leu Lys Leu Thr Asp Asp Asp Lys 
545                 550                 555                 560 


Lys Arg Ile Asn Lys Glu Asn Asn Glu Asn Arg Leu Ala Ala Glu Lys 
                565                 570                 575     


Arg Ser Glu Lys Leu Leu Thr Ile Gly Arg Pro Asp Thr Gly Ala Asn 
            580                 585                 590         


Arg Ala Leu Leu Lys Leu Trp Glu Glu Leu Asn Pro Ala Asn Val Leu 
        595                 600                 605             


Asp Arg Arg Cys Ile Tyr Thr Gly Thr Gln Ile Ser Val Asp Met Leu 
    610                 615                 620                 


Phe Ser Ala Ala Val Glu Val Asp His Ile Leu Pro Phe Thr Ala Thr 
625                 630                 635                 640 


Leu Asp Asp Ser Asn Gly Asn Lys Ile Val Cys Met Arg Glu Ala Asn 
                645                 650                 655     


Arg Thr Lys Arg Lys Gln Ser Pro Tyr Glu Ala Trp Gly His Thr Asp 
            660                 665                 670         


Arg Trp Glu Glu Ile Ala Ala Arg Ala Ala Gln Leu Pro Arg Asn Lys 
        675                 680                 685             


Arg Trp Arg Phe Glu Pro Asp Ala Met Glu Lys Phe Lys Asp Glu Ser 
    690                 695                 700                 


Gly Phe Gln Ala Arg His Leu Val Asp Thr Gln Tyr Leu Ala Arg Leu 
705                 710                 715                 720 


Ser Arg Glu Tyr Leu Glu Thr Leu Tyr Pro Gly Ser Gly Glu Gly Ser 
                725                 730                 735     


Ser His Ile Trp Val Ser Pro Gly Arg Leu Thr Glu Met Val Arg Arg 
            740                 745                 750         


Lys Leu Gly Leu Asn Asp Leu Leu Pro Asp His Asn Phe Gly Gly Gly 
        755                 760                 765             


Ala Asp Gln Ala Lys Asn Arg Leu Asp His Arg His His Ala Ile Asp 
    770                 775                 780                 


Ala Val Val Val Ala Ile Val Asp Arg Ser Met Leu Thr Glu Ile Ser 
785                 790                 795                 800 


Arg Val Ser Gly Leu Glu Gly Ala Glu Gly Arg Glu Arg Ile Ile Ile 
                805                 810                 815     


Pro Pro Pro Trp Asp Glu Phe Arg Asn Asp Leu Arg Thr Ala Val Asn 
            820                 825                 830         


Ala Ile Thr Val Ser His Arg Thr Asp His Gly Thr Ile Ser Lys Ala 
        835                 840                 845             


Gly Leu Pro Lys Gly Lys Asp Ala Thr Ala Gly Arg Leu His Asn Asp 
    850                 855                 860                 


Thr Ala Tyr Gly Phe Thr Gly Glu Thr Asp Ala Lys Gly Asn Ser Ile 
865                 870                 875                 880 


Val Val Arg Arg Val Pro Leu Ala Ser Phe Lys Lys Leu Ala Asp Leu 
                885                 890                 895     


Asp Arg Ile Arg Asp Thr Asp Leu Lys Ala Ala Leu Tyr Gln His Leu 
            900                 905                 910         


Glu Gly Leu Glu Gly Lys Ala Phe Glu Lys Ala Val Met Asp Phe Pro 
        915                 920                 925             


Asn Leu Glu Trp His Pro Phe Gly Asn Val Arg Arg Val Arg Val Thr 
    930                 935                 940                 


Glu Val Leu Ser Val Ile Pro Ile Ser Asp Gln Asn Gly Arg Ile Tyr 
945                 950                 955                 960 


Lys Gly Tyr Lys Gly Asp Ser Asn Tyr Arg Tyr Asp Val Trp Glu Leu 
                965                 970                 975     


Pro Gly Gly Lys Trp Glu Ala Glu Val Val Ser Thr Phe Asp Ala His 
            980                 985                 990         


Gln Pro Asp Trp Val Ser Lys Ile  Arg Ser Arg Ser Pro  Thr Ala Arg 
        995                 1000                 1005             


Lys Val  Leu Ser Leu Gln Gln  Asn Asp Leu Ile Ala  Val Glu Arg 
    1010                 1015                 1020             


Ala His  Glu Thr Arg Glu Leu  Met Arg Val Val Lys  Phe Arg Gln 
    1025                 1030                 1035             


Asn Gly  Glu Ile Thr Phe Ala  Pro Pro Gln Glu Ala  Gly Ser Leu 
    1040                 1045                 1050             


Lys Arg  Arg Asp Ala Thr Pro  Asn Glu Asp Asp Pro  Phe Lys Tyr 
    1055                 1060                 1065             


Phe Ala  Val Ala Ala Ser Ser  Leu Gln Lys Ala Lys  Ala Arg Gln 
    1070                 1075                 1080             


Val Arg  Ile Asp Glu Leu Gly  Arg Val Leu Asp Pro  Gly Phe Pro 
    1085                 1090                 1095             


Ala Arg  Thr Ala Arg Arg Lys  Thr Arg Lys Ser Thr  
    1100                 1105                 1110 


<210>  653
<211>  1110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  653

Met Arg Ile Leu Gly Phe Asp Ile Gly Ile Thr Ser Ile Gly Trp Ala 
1               5                   10                  15      


Tyr Val Glu Gly Glu Glu Leu Lys Asp Cys Gly Val Arg Ile Phe Thr 
            20                  25                  30          


Lys Ala Glu Asn Pro Gln Asn Gly Asp Ser Leu Ala Ala Pro Arg Arg 
        35                  40                  45              


Glu Ala Arg Gly Val Arg Arg Arg Leu Ala Arg Arg Lys Val Arg Leu 
    50                  55                  60                  


Asn Val Ile Lys Arg Leu Leu Cys Lys Glu Phe Gly Leu Asn Leu Ser 
65                  70                  75                  80  


Asp Tyr Phe Ala Asn Asp Gly Glu Leu Pro Lys Ala Tyr Glu Thr Thr 
                85                  90                  95      


Lys Asp Thr Lys Ser Pro Tyr Glu Leu Arg Ser Leu Ala Leu Glu Gln 
            100                 105                 110         


Lys Leu Glu Pro Lys Glu Leu Ala Arg Val Ile Leu His Ile Ala Lys 
        115                 120                 125             


His Arg Gly Tyr Gly Asn Lys His Ala Lys Arg Asp Leu Glu Ala Glu 
    130                 135                 140                 


Glu Lys Ala Lys Lys Ala Ala Glu Lys Ala Pro Gln Glu Lys Glu Ser 
145                 150                 155                 160 


Thr Thr Thr Asn Gly Asp Ser Lys Lys Glu Gln Glu Lys Val Leu Lys 
                165                 170                 175     


Ala Leu Tyr Gln Asn Glu Thr Leu Leu Lys Lys Tyr Gln Thr Asn Tyr 
            180                 185                 190         


Gln Thr Asn Asn Lys Thr Asn Asp Glu Thr Asn Asn Lys Thr Ile Phe 
        195                 200                 205             


Leu Val Gly Lys Tyr Leu Tyr Lys Glu Phe Gln Asp Lys Gly Gln Arg 
    210                 215                 220                 


Ile Arg Asn Thr Thr Asn Asn Tyr Gln His Thr Met Arg Gln Glu Trp 
225                 230                 235                 240 


Leu Lys Asp Glu Leu Glu Phe Ile Phe Lys Lys Gln Lys Asp Phe Gly 
                245                 250                 255     


Ala Thr Phe Ser Glu Asn Phe Glu Ser Gln Ile Leu Glu Thr Ala Phe 
            260                 265                 270         


Tyr Gln Arg Asp Leu Lys Ser Phe Glu Asp Lys Val Gly Lys Cys Val 
        275                 280                 285             


Phe Tyr Glu Asn Glu Asn Arg Ala Pro Lys Asp Ser Leu Ser Ala Met 
    290                 295                 300                 


Glu Phe Val Ala Leu Thr Arg Ile Ile Asn Thr Leu Lys Asn Leu Glu 
305                 310                 315                 320 


Lys Lys Ser Lys Asn Leu Gly Ile Gly Glu Thr Tyr Gly Lys Asp Lys 
                325                 330                 335     


Ile Gln Glu Ile Leu Lys Ile Val Leu Asp Lys Gly Glu Met Ser Tyr 
            340                 345                 350         


Lys Glu Leu Arg Lys Ile Leu Lys Leu Asn Glu Ser Ile Leu Phe Ala 
        355                 360                 365             


Lys Asp Ser Lys Leu Asp Tyr Thr Lys Gly Met Gln Glu Ala Glu Lys 
    370                 375                 380                 


Ala Lys Phe Ile Glu Leu Lys Asn Leu Lys Ala Phe Lys Lys Ala Met 
385                 390                 395                 400 


Gly Gly Asp Phe Ser Lys Phe Pro Arg Glu Glu Leu Asp Lys Ile Ala 
                405                 410                 415     


Thr Asp Ile Val Leu Ile Lys Ser Lys Glu Asn Leu Ala Lys Lys Leu 
            420                 425                 430         


Gln Asn Tyr Ser Ala Leu Ser Lys Glu Gln Val Glu Ala Leu Ser Asn 
        435                 440                 445             


Leu Asn Phe Ala Lys His Ile Asp Leu Ser Leu Lys Ala Leu Asp Lys 
    450                 455                 460                 


Ile Leu Pro Phe Met Arg Glu Gly Leu Ser Tyr Asp Glu Val Val Lys 
465                 470                 475                 480 


Lys Val Gly Leu Gln Glu His Arg Lys Tyr Lys Gln Lys Gly Glu Phe 
                485                 490                 495     


Leu Ile Pro Leu Lys Glu Tyr Glu Pro Tyr Leu Ala Asn Pro Val Val 
            500                 505                 510         


Ala Arg Ala Leu Ser Glu Tyr Arg Lys Val Leu Asn Ala Leu Leu Arg 
        515                 520                 525             


Lys Tyr Gly Asn Val His Lys Ile His Leu Glu Phe Thr Arg Glu Ala 
    530                 535                 540                 


Gly Lys Asn Tyr Asp Glu Arg Lys Lys Ile Glu Lys Glu Gln Arg Glu 
545                 550                 555                 560 


His Phe Glu Ala Asn Gln Lys Ala Ile Lys Gln Arg Glu Ile Leu Gly 
                565                 570                 575     


Leu Pro Ile Asn Gly Ala Asn Ile Leu Lys Met Lys Leu Trp Ile Glu 
            580                 585                 590         


Gln Gly Glu Cys Cys Val Tyr Ser Gly Glu Lys Ile Thr Ser Lys His 
        595                 600                 605             


Phe Phe Asp Ser Asn Ala Leu Gln Val Asp His Ile Tyr Pro Tyr Ser 
    610                 615                 620                 


Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu Val Phe Ala Lys 
625                 630                 635                 640 


Glu Asn Gln Asn Lys Leu Asn Lys Thr Pro Phe Glu Ala Phe Gly Ser 
                645                 650                 655     


Asp Lys Glu Lys Trp Asp Arg Ile Leu Ser Leu Ser Ala Lys Leu Pro 
            660                 665                 670         


Lys Gln Lys Gln Arg Arg Ile Ser Asn Lys Asp Phe Lys Asp Lys Glu 
        675                 680                 685             


Leu Gly Phe Ile Ala Arg Asn Ile Asn Asp Thr Gly Tyr Ile Ala Arg 
    690                 695                 700                 


Leu Ala Ser Gln Trp Thr Asn Asn Cys Leu Lys Phe Glu Asp Leu Arg 
705                 710                 715                 720 


Glu Asn Glu Val Thr Ile Ala Gly Glu Lys Gly Ser Lys Asn His Leu 
                725                 730                 735     


Glu Val Ile Ser Gly Gln Leu Thr Ser Met Leu Arg His Tyr Trp Gly 
            740                 745                 750         


Leu Gly Asp Lys Asp Arg Asn Asn His Leu His His Ala Val Asp Ala 
        755                 760                 765             


Val Ile Ile Ala Tyr Thr Asn Ala Lys Thr Ile Lys Ala Phe Ala Asp 
    770                 775                 780                 


Phe Lys Lys Asn Gln Glu Gln Asn Arg Ala Lys Phe Tyr Ala Lys Gln 
785                 790                 795                 800 


Ile Ser Glu Gln Glu Tyr Lys Arg Gln Arg Asn Pro Phe Glu Pro Cys 
                805                 810                 815     


Ala Asn Phe Arg Gln Arg Val Glu Glu Lys Ile Asn Lys Ile Phe Val 
            820                 825                 830         


Ser Lys Pro Pro Arg Lys Arg Ala Arg Gly Ala Leu His Lys Glu Thr 
        835                 840                 845             


Phe Gly Ser Ile Cys Leu Asp Gly Asn Asn Asn Trp Val Ala Arg Phe 
    850                 855                 860                 


Lys Ile Lys Ser Lys Lys Glu Gln Glu Asp Ile Thr Lys Glu Lys Ala 
865                 870                 875                 880 


Thr Lys Ala Lys Tyr Lys Asp Glu Phe Lys Gly Glu Glu Asn Ile Arg 
                885                 890                 895     


Lys Ala Leu Lys Leu Gly Lys Ile Arg Arg Ile Asn Asn Thr Gln Lys 
            900                 905                 910         


Asp Ile Ala Ile Gly Asp Lys Ile Val Lys Asn Glu Thr Met Val Arg 
        915                 920                 925             


Val Asp Ile Phe Lys Asp Lys Lys Gly Lys Phe Tyr Ala Val Pro Ile 
    930                 935                 940                 


Tyr Thr Met Asp Phe Ala Leu Gly Ile Leu Pro Asn Lys Ala Val Ala 
945                 950                 955                 960 


Thr Gly Glu Asp Glu Asp Gly Val Ile Lys Asp Trp Ile Glu Met Asp 
                965                 970                 975     


Lys Thr Lys Tyr Glu Phe Cys Phe Ser Leu Phe Lys Asp Asp Leu Ile 
            980                 985                 990         


Lys Val Arg Lys Lys Lys Met Lys  Glu Ala Glu Leu Cys  Tyr Phe Val 
        995                 1000                 1005             


Gly Phe  Asp Thr Asp Gly Ser  Arg Ile Ile Val Ala  Lys His Asn 
    1010                 1015                 1020             


Asn Asp  Tyr Gln Thr Met Thr  Glu Asn Glu Cys Lys  Leu Phe Thr 
    1025                 1030                 1035             


Leu Lys  Glu Lys Lys Lys Lys  Gln Lys Thr Leu Asn  Asp Glu Glu 
    1040                 1045                 1050             


Asp Asn  Gln Glu Arg Ile Glu  Thr Glu Tyr Lys Ile  Ile Gly Gly 
    1055                 1060                 1065             


Arg Thr  Gly Ile Gln Asn Leu  Glu Thr Phe Glu Lys  His Gln Val 
    1070                 1075                 1080             


Ser Pro  Leu Gly Glu Val Gln  Lys Ala Glu Phe Glu  Asp Arg Gln 
    1085                 1090                 1095             


Glu Ile  Lys Leu Lys Ser Ser  Pro Lys Gly Lys Asp  
    1100                 1105                 1110 


<210>  654
<211>  1110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  654

Met Ser Glu Lys Tyr Arg Leu Gly Leu Asp Val Gly Thr Asn Ser Leu 
1               5                   10                  15      


Gly Trp Ala Val Ile Gly Leu Lys Glu Asp Val Pro Ser Ser Val Leu 
            20                  25                  30          


Ala Thr Gly Val Arg Ile Phe Ser Glu Gly Arg Asp Ala Lys Thr Lys 
        35                  40                  45              


Ser Thr Leu Lys Ala Ser Arg Thr Glu Lys Arg Ser Ala Arg Arg Arg 
    50                  55                  60                  


Arg Asp Arg Phe Leu Gln Arg Gln Thr Phe Leu Ile Ser Glu Met Val 
65                  70                  75                  80  


Lys Ser Gly Leu Phe Pro Lys Asp Ile Val Glu Gln Lys Asn Leu Glu 
                85                  90                  95      


Leu Leu Asp Pro Tyr Glu Ile Arg Phe Lys Ala Leu Thr Glu Gln Val 
            100                 105                 110         


Pro Leu His His Leu Gly Arg Ala Leu Phe His Ile Asn Gln Arg Arg 
        115                 120                 125             


Gly Phe Lys Ser Ser Arg Lys Asp Lys Ser Glu Glu Thr Thr Ser Gly 
    130                 135                 140                 


Val Val Ser Lys Ser Val Arg Ala Leu Tyr Glu Glu Met Gln Leu Ile 
145                 150                 155                 160 


Asn Pro Ala Pro Val Ile Asp Asp Pro Lys Ala Leu Ser Lys Glu Glu 
                165                 170                 175     


Arg Arg Glu Ile Arg Val Gln Glu Ala Ser Ser Arg Glu Glu Ala Leu 
            180                 185                 190         


Val Thr Leu Ala Glu Gln Lys Gly Val Thr Phe Gly Ala Phe Leu Tyr 
        195                 200                 205             


Arg Arg Leu Gln Ser Gly Lys Ser Val Arg Ala Arg Lys Asp Ala Asp 
    210                 215                 220                 


Ser Gln Leu Tyr Asp Val Tyr Pro Thr Arg Glu Leu Leu Lys Asp Glu 
225                 230                 235                 240 


Tyr Ser Lys Leu Met Gln His Gln Ala Gln Tyr Tyr Pro Asn Val Ile 
                245                 250                 255     


Asn Glu Ala Leu Ile Ser Arg Leu His Asp Val Ile Phe Phe Gln Arg 
            260                 265                 270         


Lys Leu Lys Glu Gln Glu Arg Gly Phe Cys Thr Tyr Tyr Pro Ser Glu 
        275                 280                 285             


Arg Arg Thr Phe Arg Cys Met Pro Ser Phe Gln Gln Tyr Arg Met Val 
    290                 295                 300                 


Gln Glu Leu Asn Ala Leu Glu Trp Val Asp Ala Ala Gly Lys His Cys 
305                 310                 315                 320 


Leu Arg Asp Tyr Pro Glu Ser Arg Asp Leu Ile Leu Ser Glu Leu Glu 
                325                 330                 335     


Asn Ile Ser Thr Lys Ala Gly Glu Phe Thr Phe Gly Asn Met Lys Lys 
            340                 345                 350         


Ile Leu Lys Lys Met Gly Val Ile Asp Gly Glu Val Ser Phe Asn Phe 
        355                 360                 365             


Glu Gly Pro Lys Arg Lys Gly Phe Gln Gly Asn Val Thr Ser Arg Glu 
    370                 375                 380                 


Met Arg His Glu Asp Cys Phe Gly Thr Arg Trp Asp Glu Leu Ser Leu 
385                 390                 395                 400 


Asp Glu Lys Asp Asp Ile Ile Asp Lys Ile Asn Asp Asp His Leu Asp 
                405                 410                 415     


Asp Glu Asp Met Glu Arg Tyr Leu Ile Asp Thr Tyr Gly Leu Ser Ser 
            420                 425                 430         


Phe Ser Val Gly Asn Ala Ile Asn Ala Arg Leu Leu Glu Gly Thr Ala 
        435                 440                 445             


Asn Val Ser Arg Lys Ala Ala Arg Thr Leu Tyr Glu Thr Met Leu Ser 
    450                 455                 460                 


Ala Asn Cys Leu Gln Ser Asp Ala Val Ile Ala Val Ser Glu Gln Asp 
465                 470                 475                 480 


Asp Thr Phe Val Asn Pro Tyr Ser Arg Thr Gly Arg Gly Glu Leu Leu 
                485                 490                 495     


Ser Glu Leu Pro Tyr Tyr Gly Glu Ala Phe Gln Asp Gly Arg His Ile 
            500                 505                 510         


Ile Pro Gly Lys Arg Glu Pro Lys Val Ser His Asp Gln Leu Lys Phe 
        515                 520                 525             


Tyr Gly Gly Val Ser Asn Pro Thr Val His Ile Ala Leu Asn Gln Ile 
    530                 535                 540                 


Arg Leu Val Val Asn Glu Ile Ile Lys Arg Tyr Gly Leu Pro Ser Ser 
545                 550                 555                 560 


Ile Ser Ile Glu Leu Ala Arg Asn Leu Pro Glu Gly Gln Asp Gly Arg 
                565                 570                 575     


Arg Lys Ile Glu Lys Met Gln Ser Glu Asn Gln Lys Asn Asn Glu Arg 
            580                 585                 590         


Tyr Asp Gln Ile Leu Thr Glu Asn Gly Gln Ser Pro Asn Arg Asp Asn 
        595                 600                 605             


Arg Leu Arg Leu Ala Leu Trp Glu Glu Leu Gly Glu Gly Pro Thr Asp 
    610                 615                 620                 


Arg Leu Cys Val Phe Thr Gly Lys Leu Ile Glu Leu Ala Asp Leu Phe 
625                 630                 635                 640 


Asn Gly Ser Val Glu Ile Glu His Leu Ile Pro Phe Ser Val Ser Leu 
                645                 650                 655     


Asp Asp Ser Lys Ala Asn Lys Thr Val Cys Trp Lys Glu Ala Asn Arg 
            660                 665                 670         


Phe Lys Gly Asn Lys Thr Pro Tyr Glu Ala Phe Ala Asn Asn Ser Gly 
        675                 680                 685             


Asp Tyr Ser Trp Glu Glu Ile Met Glu Arg Val Arg Ala Leu Pro Asn 
    690                 695                 700                 


Ser Lys Gln Trp Arg Phe Gln Glu Asp Ala Leu Lys Ile Trp Asn Ser 
705                 710                 715                 720 


Asp His Ser Asp Phe Ser Gly Arg His Leu Asn Asp Thr Arg Tyr Ile 
                725                 730                 735     


Ser Arg Leu Ala Arg Glu Tyr Leu Glu Asn Ile Cys Pro Phe Asn Lys 
            740                 745                 750         


Ile Asp Val Val Thr Gly Arg Leu Thr Ala Leu Leu Arg Gly His Trp 
        755                 760                 765             


Gly Leu Asn Asn Ile Leu Arg Gly His Asn Glu Pro Glu Thr Ala Thr 
    770                 775                 780                 


Pro Lys Lys Met Arg Asp Asp His Arg His His Ala Ile Asp Ala Val 
785                 790                 795                 800 


Val Ile Ala Met Thr Ser Leu Thr Ile Leu Gln Lys Val Ser Ser Ala 
                805                 810                 815     


Ala Gly Lys Ala Glu Glu Leu Asp Val Gly Lys Leu Phe Ala Lys Asp 
            820                 825                 830         


Glu Ser Gly Arg Ser Val Ile Asp Pro Trp Asp Gly Phe Arg Asp Glu 
        835                 840                 845             


Val Ala Glu Lys Val Arg Asn Ile Ile Val Ser His Lys Thr Lys Arg 
    850                 855                 860                 


Lys Thr Ile Gly Asn Ser Gly Lys Ser Thr Asp Arg Ala Leu His Asn 
865                 870                 875                 880 


Glu Thr Ala Tyr Gly Leu Ile Asp Gly Pro Asp Glu Lys Gly Leu Tyr 
                885                 890                 895     


Ser Val Val Thr Thr Lys Pro Ile Ser Tyr Phe Asp Asn Arg Asp Lys 
            900                 905                 910         


Ile Glu Lys Ile Arg Asp Glu Lys Leu Arg Asp Asp Phe Leu Thr Ala 
        915                 920                 925             


Trp Asp Gly Glu Gly Asp Asn Lys Ala Ser Gln Leu Ala Val Leu Asp 
    930                 935                 940                 


Lys Ala Ser Gly Leu Asn Val Lys Lys Cys Arg Tyr Val Glu Arg Lys 
945                 950                 955                 960 


Lys Val Ile Pro Ile Asn Gly Pro Asp Gly Gln Pro Tyr Lys Ala Tyr 
                965                 970                 975     


Asp Gly Asn Ser Asn Trp Gly Met Glu Ile Tyr Glu Phe Pro Asn Gly 
            980                 985                 990         


His Pro Lys Glu Lys Lys Trp Asn  Gly Val Val Ile Ser  Thr Tyr Asp 
        995                 1000                 1005             


Ala Asn  Lys Ser Glu Phe Lys  Pro Gly Glu Thr Phe  Arg Pro His 
    1010                 1015                 1020             


Pro Ala  Ala Lys Leu Val Met  Arg Leu His Ile Asn  Asp Ile Val 
    1025                 1030                 1035             


Glu Val  Gln Asn Asp Gly Glu  Pro Ile Leu Tyr Arg  Val Gln Leu 
    1040                 1045                 1050             


Ile Ser  Ala Gly Thr Leu Thr  Val Ser Pro Leu His  Glu Ala Asn 
    1055                 1060                 1065             


Val Asp  Ser Arg Asn Arg Asp  Lys His Asp Glu Phe  Lys Tyr Lys 
    1070                 1075                 1080             


Ser Leu  Ser Val Thr Pro Met  Gln Lys Met Gly Ala  Lys Lys Val 
    1085                 1090                 1095             


Asn Ile  Ser Pro Thr Gly Leu  Lys Asn Tyr Ser Arg  
    1100                 1105                 1110 


<210>  655
<211>  1114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  655

Met Gly Ile Ile Ile Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Ile 
1               5                   10                  15      


Ala Val Val Asp Lys Asp Thr Leu Asp Ile Lys Cys Val Val Ser Asp 
            20                  25                  30          


Leu Phe Asp Ser Ala Asp Ala Ser Lys Asn Ile Glu Arg Arg Asn Asn 
        35                  40                  45              


Arg Gln Ala Arg Arg Leu Asn Arg Arg Arg Lys Thr Arg Ile Gly Asp 
    50                  55                  60                  


Phe Asn Thr Leu Trp Val Ser Met Phe Gly Glu Leu Pro Glu Glu Val 
65                  70                  75                  80  


Asp Ser Asn Val Leu Leu Leu Arg Asn Ala Gly Leu Asp Glu Gln Leu 
                85                  90                  95      


Thr Leu Asp Glu Ile Tyr Cys Val Leu Lys Tyr Met Leu Lys His Arg 
            100                 105                 110         


Gly Ile Ser Tyr Leu Glu Asp Ala Leu Asn Glu Glu Asn Val Thr Gly 
        115                 120                 125             


Ser Tyr Gln Lys Gly Ile Ala Ile Asn Gln Arg Glu Ser Glu Tyr Met 
    130                 135                 140                 


Leu Pro Cys Glu Ile Gln Leu Glu Arg Phe Arg Lys Tyr Gly Gln Tyr 
145                 150                 155                 160 


Arg Gly Glu Cys Glu Ala Glu Asn Glu Asn Gly Glu Lys Ile Thr Leu 
                165                 170                 175     


Ser Asn Val Phe Met Thr Asn Ser Tyr Arg Lys Glu Ile Asp Lys Phe 
            180                 185                 190         


Leu Asn Thr Gln Gly Lys Tyr Gly Ile Leu Asn Gln Lys Phe Ile Asp 
        195                 200                 205             


Glu Tyr Leu Lys Ile Phe Asn Arg Lys Arg Lys Tyr Tyr Glu Gly Pro 
    210                 215                 220                 


Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Lys Tyr Thr Thr Gly Lys 
225                 230                 235                 240 


Asp Leu Asp Gly Lys Tyr Ile Thr Glu Lys Asn Ile Phe Glu Lys Leu 
                245                 250                 255     


Ile Gly Lys Cys Ser Val Tyr Lys Glu Glu Leu Arg Ala Ala Gly Ala 
            260                 265                 270         


Ser Tyr Thr Ala Gln Glu Phe Asn Ile Leu Asn Asp Leu Asn Asn Ile 
        275                 280                 285             


Thr Val Asn Asn Lys Lys Leu Thr Thr Gln Gln Lys Lys Asp Val Ile 
    290                 295                 300                 


Glu Ile Val Gln Asn Ser Asp Arg Ile Asn Met Glu Lys Ile Ile Ser 
305                 310                 315                 320 


Ser Cys Ile Gly Glu Lys Ile Glu Lys Ile Glu Gly Ala Arg Ile Asp 
                325                 330                 335     


Lys Asn Glu Lys Asn Ile Tyr His Thr Phe Glu Gln Tyr Asn Lys Met 
            340                 345                 350         


Arg Lys Ile Phe Ala Glu Lys Asn Tyr Asp Ile Lys Glu Leu Thr Ile 
        355                 360                 365             


Glu Gln Leu Asp Glu Ile Gly Arg Ile Leu Thr Ile Asn Thr Glu Arg 
    370                 375                 380                 


Glu Gly Ile Lys Asp Ala Ile Met Glu Ser Glu Leu Leu His Leu Asn 
385                 390                 395                 400 


Asn Glu Gln Ile Asn Leu Leu Ile Glu Leu Arg Tyr Lys Asn Ser Ser 
                405                 410                 415     


Leu Phe Thr Lys Trp Gln Ser Leu Ser Ile Lys Ala Met Arg Glu Ile 
            420                 425                 430         


Ile Pro Asp Leu Tyr Asp Arg Asn Val Asn Gln Met Asn Ile Leu Ala 
        435                 440                 445             


Glu Arg Gly Val Phe Lys Asp Lys Ile Asn Leu Phe Glu Asn Ser Val 
    450                 455                 460                 


Tyr Leu Pro Lys Glu Pro Val Leu Glu Glu Ile Tyr Asn Pro Val Val 
465                 470                 475                 480 


Arg Arg Ser Ile Ser Met Thr Ile Asp Ala Leu Asn Ala Leu Ile Lys 
                485                 490                 495     


Lys Tyr Gly Tyr Pro Glu Asp Ile Val Ile Glu Met Pro Arg Asp Arg 
            500                 505                 510         


Asn Asp Ala Glu Gln Lys Asp Arg Ile Lys Lys Phe Gln Lys Thr Arg 
        515                 520                 525             


Glu Asn Glu Leu Lys Asn Ile Lys Lys Lys Ile Lys Glu Glu Tyr Gly 
    530                 535                 540                 


Ile Glu Ile Thr Asp Glu Tyr Phe Arg Lys His Lys Gly Leu Glu Leu 
545                 550                 555                 560 


Glu Leu Lys Leu Trp Val Glu Gln Asn Glu Lys Cys Pro Tyr Ser Gly 
                565                 570                 575     


Lys Tyr Ile Asn Val Gln Asp Ile Leu Tyr His His Glu Leu Phe Glu 
            580                 585                 590         


Ile Asp His Ile Ile Pro Leu Ser Ile Ser Tyr Asp Asp Ser Arg Asn 
        595                 600                 605             


Asn Lys Val Leu Val Tyr Lys Glu Glu Asn Gln Asp Lys Gly Asn Arg 
    610                 615                 620                 


Thr Pro Leu Met Tyr Leu Ser Thr Val Asn Arg Gln Tyr Asp Gln His 
625                 630                 635                 640 


Glu Phe Met Asn Tyr Val Leu Ser Thr Lys Thr Leu Pro Arg Lys Lys 
                645                 650                 655     


Arg Glu Leu Leu Leu Asn Tyr Glu Asp Ile Thr Lys Ile Asp Val Leu 
            660                 665                 670         


Lys Gly Phe Ile Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ala Ser Arg 
        675                 680                 685             


Ser Val Leu Asn Ala Ile Gln Leu Phe Phe Gly Gly Lys Glu Cys Asp 
    690                 695                 700                 


Thr Lys Val Arg Val Ile Arg Gly Ala Val Thr Asn Gln Leu Arg Ile 
705                 710                 715                 720 


Lys Leu Asn Leu Pro Lys Asp Arg Glu Lys Ser Tyr Ser His His Ala 
                725                 730                 735     


Ile Asp Ala Ala Ile Met Cys Tyr Ser Gln Met Gly Leu Met Asn Phe 
            740                 745                 750         


Arg Asn Ile Gln Asn Lys Phe Ile Asp Phe Glu Asn Glu Thr Tyr Leu 
        755                 760                 765             


Ser Asp Glu Arg Asp Ser Cys Phe Asp Asn Lys Tyr Tyr Asp Glu Ala 
    770                 775                 780                 


Leu Phe Gly Gln Val Leu Lys Met Arg Lys Asn Leu Leu Lys Ala Glu 
785                 790                 795                 800 


Gly Lys Pro Gln Arg Ser Val Gln Ser Lys Gly Val Phe Asp Glu Glu 
                805                 810                 815     


Glu Lys Asn Asn Gly Ile Val Arg Tyr His His Lys Val Asp Lys Lys 
            820                 825                 830         


Pro Asn Arg Gly Leu Ser Asn Ala Ser Ile Tyr Gly Thr Arg Lys Ile 
        835                 840                 845             


Asp Asp Lys Ile Tyr Lys Ile Val Gly Tyr Asp Ile Arg Asn Asn Lys 
    850                 855                 860                 


Asp Ala Glu Ala Ile Ile Asn Ile Ile Lys Lys Gly Lys Ser Asp Asn 
865                 870                 875                 880 


Phe Leu Met Ala Lys His Asp Pro Glu Thr Phe Lys Asn Leu Met Glu 
                885                 890                 895     


Ile Tyr Glu Gln Tyr Lys Glu Glu Lys Asn Pro Phe Leu Ala Tyr Glu 
            900                 905                 910         


Gln Glu Thr Gly Asp Tyr Ile Arg Lys Tyr Ala Lys Asn His Asn Gly 
        915                 920                 925             


Pro Ile Ile Lys Lys Ile Lys Tyr Val Asp Ser Glu Val Gly Ser Cys 
    930                 935                 940                 


Ile Asp Ile Ser Ala Asn Tyr Gly Tyr Lys Asn Gly Asp Lys Arg Val 
945                 950                 955                 960 


Ile Met Asp Thr Leu Thr Pro Tyr Arg Ala Asp Val Tyr Tyr Asn Tyr 
                965                 970                 975     


Lys Asp Gly Thr Tyr Arg Ile Ile Gly Val Lys Tyr Ala Asp Phe Lys 
            980                 985                 990         


Tyr Ser Asn Ala Lys Tyr Val Leu  Asp Glu Glu Ala Tyr  Ala Leu Ile 
        995                 1000                 1005             


Leu Ile  Lys Glu Gly Leu Leu  Lys Ile Gly Asp Ser  Leu Glu Asn 
    1010                 1015                 1020             


Ile Ser  Ser Ser Gly Tyr Glu  Tyr Arg Leu Ser Phe  Tyr Lys Asn 
    1025                 1030                 1035             


Asp Ile  Ile Glu Tyr Thr Lys  Asn Asp Ile Val Tyr  Lys Glu Arg 
    1040                 1045                 1050             


Phe Leu  Ser Arg Thr Met Pro  Lys Val Lys Asn Tyr  Ile Glu Thr 
    1055                 1060                 1065             


Lys Pro  Ile Ser Ala Ala Leu  Tyr Glu Lys Arg Asn  Pro Ile Gly 
    1070                 1075                 1080             


Leu Thr  Asn Ala Thr Asn Ile  Cys Lys Ile Tyr Thr  Asp Val Leu 
    1085                 1090                 1095             


Gly Asn  Glu Thr Lys Ile Tyr  Lys Glu Lys Phe Lys  Leu Ser Val 
    1100                 1105                 1110             


Asp 
    


<210>  656
<211>  1114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  656

Met Asn Tyr Thr Glu Lys Glu Lys Leu Phe Met Lys Tyr Ile Leu Ala 
1               5                   10                  15      


Leu Asp Ile Gly Ile Ala Ser Val Gly Trp Ala Ile Leu Asp Lys Glu 
            20                  25                  30          


Ser Glu Thr Val Ile Glu Ala Gly Ser Asn Ile Phe Pro Glu Ala Ser 
        35                  40                  45              


Ala Ala Asp Asn Gln Leu Arg Arg Asp Met Arg Gly Ala Lys Arg Asn 
    50                  55                  60                  


Asn Arg Arg Leu Lys Thr Arg Ile Asn Asp Phe Ile Lys Leu Trp Glu 
65                  70                  75                  80  


Asn Asn Asn Leu Ser Ile Pro Gln Phe Lys Ser Thr Glu Ile Val Gly 
                85                  90                  95      


Leu Lys Val Arg Ala Ile Thr Glu Glu Ile Thr Leu Asp Glu Leu Tyr 
            100                 105                 110         


Leu Ile Leu Tyr Ser Tyr Leu Lys His Arg Gly Ile Ser Tyr Leu Glu 
        115                 120                 125             


Asp Ala Leu Asp Asp Thr Val Ser Gly Ser Ser Ala Tyr Ala Asn Gly 
    130                 135                 140                 


Leu Lys Leu Asn Ala Lys Glu Leu Glu Thr His Tyr Pro Cys Glu Ile 
145                 150                 155                 160 


Gln Gln Glu Arg Leu Asn Thr Ile Gly Lys Tyr Arg Gly Gln Ser Gln 
                165                 170                 175     


Ile Ile Asn Glu Asn Gly Glu Val Leu Asp Leu Ser Asn Val Phe Thr 
            180                 185                 190         


Ile Gly Ala Tyr Arg Lys Glu Ile Gln Arg Val Phe Glu Ile Gln Lys 
        195                 200                 205             


Lys Tyr His Pro Glu Leu Thr Asp Glu Phe Cys Asp Gly Tyr Met Leu 
    210                 215                 220                 


Ile Phe Asn Arg Lys Arg Lys Tyr Tyr Glu Gly Pro Gly Asn Glu Lys 
225                 230                 235                 240 


Ser Arg Thr Asp Tyr Gly Arg Phe Thr Thr Lys Leu Asp Ala Asn Gly 
                245                 250                 255     


Asn Tyr Ile Thr Glu Asp Asn Ile Phe Glu Lys Leu Ile Gly Lys Cys 
            260                 265                 270         


Ser Val Tyr Pro Asp Glu Leu Arg Ala Ala Ala Ala Ser Tyr Thr Ala 
        275                 280                 285             


Gln Glu Tyr Asn Val Leu Asn Asp Leu Asn Asn Leu Thr Ile Asn Gly 
    290                 295                 300                 


Arg Lys Leu Glu Glu Asn Glu Lys His Glu Ile Val Glu Arg Ile Lys 
305                 310                 315                 320 


Ser Ser Asn Thr Ile Asn Met Arg Lys Ile Ile Ser Asp Cys Met Gly 
                325                 330                 335     


Glu Asn Ile Asp Asp Phe Ala Gly Ala Arg Ile Asp Lys Ser Gly Lys 
            340                 345                 350         


Glu Ile Phe His Lys Phe Glu Val Tyr Asn Lys Met Arg Lys Ala Leu 
        355                 360                 365             


Leu Glu Ile Gly Ile Asp Ile Ser Asn Tyr Ser Arg Glu Glu Leu Asp 
    370                 375                 380                 


Glu Ile Gly Tyr Ile Met Thr Ile Asn Thr Asp Lys Glu Ala Met Met 
385                 390                 395                 400 


Glu Ala Phe Gln Lys Ser Trp Ile Asp Leu Ser Asp Asp Val Lys Gln 
                405                 410                 415     


Cys Leu Ile Asn Met Arg Lys Thr Asn Gly Ala Leu Phe Asn Lys Trp 
            420                 425                 430         


Gln Ser Phe Ser Leu Lys Ile Met Asn Glu Leu Ile Pro Glu Met Tyr 
        435                 440                 445             


Ala Gln Pro Lys Glu Gln Met Thr Leu Leu Thr Glu Met Gly Val Thr 
    450                 455                 460                 


Lys Gly Thr Gln Glu Glu Phe Ala Gly Leu Lys Tyr Ile Pro Val Asp 
465                 470                 475                 480 


Val Val Ser Glu Asp Ile Phe Asn Pro Val Val Arg Arg Ser Val Arg 
                485                 490                 495     


Ile Ser Phe Lys Ile Leu Asn Ala Val Leu Lys Lys Tyr Lys Ala Leu 
            500                 505                 510         


Asp Thr Ile Val Ile Glu Met Pro Arg Asp Arg Asn Ser Glu Glu Gln 
        515                 520                 525             


Lys Lys Arg Ile Asn Asp Ser Gln Lys Leu Asn Glu Lys Glu Met Glu 
    530                 535                 540                 


Tyr Ile Glu Lys Lys Leu Ala Val Thr Tyr Gly Ile Lys Leu Ser Pro 
545                 550                 555                 560 


Ser Asp Phe Ser Ser Gln Lys Gln Leu Ser Leu Lys Leu Lys Leu Trp 
                565                 570                 575     


Asn Glu Gln Asp Gly Ile Cys Leu Tyr Ser Gly Lys Thr Ile Asp Pro 
            580                 585                 590         


Asn Asp Ile Ile Asn Asn Pro Gln Leu Phe Glu Ile Asp His Ile Ile 
        595                 600                 605             


Pro Arg Ser Ile Ser Phe Asp Asp Ala Arg Ser Asn Lys Val Leu Val 
    610                 615                 620                 


Tyr Arg Ser Glu Asn Gln Lys Lys Gly Asn Gln Thr Pro Tyr Tyr Tyr 
625                 630                 635                 640 


Leu Thr His Ser His Ser Glu Trp Ser Phe Glu Gln Tyr Lys Ala Thr 
                645                 650                 655     


Val Met Asn Leu Ser Lys Lys Lys Glu Tyr Ala Ile Ser Arg Lys Lys 
            660                 665                 670         


Ile Gln Asn Leu Leu Tyr Ser Glu Asp Ile Thr Lys Met Asp Val Leu 
        675                 680                 685             


Lys Gly Phe Ile Asn Arg Asn Ile Asn Asp Thr Ser Tyr Ala Ser Arg 
    690                 695                 700                 


Leu Val Leu Asn Thr Ile Gln Asn Phe Phe Met Ala Asn Glu Ala Asp 
705                 710                 715                 720 


Thr Lys Val Lys Val Ile Lys Gly Ser Tyr Thr His Gln Met Arg Cys 
                725                 730                 735     


Asn Leu Lys Leu Asp Lys Asn Arg Asp Glu Ser Tyr Ser His His Ala 
            740                 745                 750         


Val Asp Ala Met Leu Ile Gly Tyr Ser Glu Leu Gly Tyr Glu Ala Tyr 
        755                 760                 765             


His Lys Leu Gln Gly Glu Phe Ile Asp Phe Glu Thr Gly Glu Ile Leu 
    770                 775                 780                 


Arg Lys Asp Met Trp Asp Glu Asn Met Ser Asp Glu Val Tyr Ala Asp 
785                 790                 795                 800 


Tyr Leu Tyr Gly Lys Lys Trp Ala Asn Ile Arg Asn Glu Val Val Lys 
                805                 810                 815     


Ala Glu Lys Asn Val Lys Tyr Trp His Tyr Val Met Arg Lys Ser Asn 
            820                 825                 830         


Arg Gly Leu Cys Asn Gln Thr Ile Arg Gly Thr Arg Glu Tyr Asp Gly 
        835                 840                 845             


Lys Gln Tyr Lys Ile Asn Lys Leu Asp Ile Arg Thr Lys Glu Gly Ile 
    850                 855                 860                 


Lys Val Phe Ala Lys Leu Ala Phe Ser Lys Lys Asp Ser Asp Arg Glu 
865                 870                 875                 880 


Arg Leu Leu Val Tyr Leu Asn Asp Arg Arg Thr Phe Asp Asp Leu Cys 
                885                 890                 895     


Lys Ile Tyr Glu Asp Tyr Ser Asp Ala Ala Asn Pro Phe Val Gln Tyr 
            900                 905                 910         


Glu Lys Glu Thr Gly Asp Ile Ile Arg Lys Tyr Ser Lys Lys His Asn 
        915                 920                 925             


Gly Pro Arg Ile Asp Lys Leu Lys Tyr Lys Asp Gly Glu Val Gly Ala 
    930                 935                 940                 


Cys Ile Asp Ile Ser His Lys Tyr Gly Phe Glu Lys Gly Ser Lys Lys 
945                 950                 955                 960 


Val Ile Leu Glu Ser Leu Val Pro Tyr Arg Met Asp Val Tyr Tyr Lys 
                965                 970                 975     


Glu Glu Asn His Ser Tyr Tyr Leu Val Gly Val Lys Gln Ser Asp Ile 
            980                 985                 990         


Lys Phe Glu Lys Gly Arg Asn Val  Ile Asp Glu Glu Ala  Tyr Ala Arg 
        995                 1000                 1005             


Ile Leu  Val Asn Glu Lys Met  Ile Gln Pro Gly Gln  Ser Arg Ala 
    1010                 1015                 1020             


Asp Leu  Glu Asn Leu Gly Phe  Lys Phe Lys Leu Ser  Phe Tyr Lys 
    1025                 1030                 1035             


Asn Asp  Ile Ile Glu Tyr Glu  Lys Asp Gly Lys Ile  Tyr Thr Glu 
    1040                 1045                 1050             


Arg Leu  Val Ser Arg Thr Met  Pro Lys Gln Arg Asn  Tyr Ile Glu 
    1055                 1060                 1065             


Thr Lys  Pro Ile Asp Lys Ala  Lys Phe Glu Lys Gln  Asn Leu Val 
    1070                 1075                 1080             


Gly Leu  Gly Lys Thr Lys Phe  Ile Lys Lys Tyr Arg  Tyr Asp Ile 
    1085                 1090                 1095             


Leu Gly  Asn Lys Tyr Ser Cys  Ser Glu Glu Lys Phe  Thr Ser Phe 
    1100                 1105                 1110             


Cys 
    


<210>  657
<211>  1115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  657

Met Ser Ala Thr Tyr Thr Ile Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Trp Cys Ala Phe Gly Glu Thr His Ile Leu Asp Leu Gly Val Arg 
            20                  25                  30          


Ala Phe Asp Lys Ala Glu Thr Ala Lys Glu Gly Glu Ser Leu Asn Leu 
        35                  40                  45              


Ala Arg Arg Asn Ala Arg Leu Met Arg Arg Arg Leu Phe Arg Arg Thr 
    50                  55                  60                  


Trp Arg Leu Lys Lys Leu Ala Arg Gln Leu Lys Ser Glu Gly Leu Ile 
65                  70                  75                  80  


Ala Asp Thr Lys Leu Phe Lys Pro Glu Leu Pro Phe Ser Lys Ser Leu 
                85                  90                  95      


Trp Gln Leu Arg Val Asp Gly Leu Asp Arg Arg Leu Asn Asp Glu Glu 
            100                 105                 110         


Trp Ala Arg Val Ile Tyr His Leu Cys Lys His Arg Gly Phe His Trp 
        115                 120                 125             


Ile Ser Arg Ala Glu Ala Lys Gln Ala Glu Gly Asp Ser Lys Ser Glu 
    130                 135                 140                 


Gly Gly Lys Val Lys Gln Gly Leu Ala Gly Thr Ala Lys Leu Met Asp 
145                 150                 155                 160 


Glu Lys Gly Tyr Arg Ser Ala Ala Glu Met Val Leu Ala Glu Phe Pro 
                165                 170                 175     


Gln Ala Gln Arg Asn Lys Gln Gly Glu Tyr Gln Lys Ala Leu Ser Arg 
            180                 185                 190         


Glu Leu Leu Ala Lys Glu Leu Ala Leu Leu Phe Ala Gln Gln Arg Glu 
        195                 200                 205             


Tyr Gly Asn Leu His Thr Pro Gly Ala Leu Glu Thr Ala Ile Leu Gly 
    210                 215                 220                 


Asn Gly Asp Lys Lys Ser Gly Leu Phe Trp Gln Gln Lys Pro Ala Leu 
225                 230                 235                 240 


Ala Gly Thr Asp Leu Leu Lys Met Leu Gly Thr Cys Thr Phe Glu Lys 
                245                 250                 255     


Gly Glu Tyr Arg Ala Pro Lys Ala Ser Phe Thr Ala Glu Arg His Val 
            260                 265                 270         


Trp Leu Thr Arg Leu Asn Asn Leu Arg Ile Val Thr Asp Gly Val Thr 
        275                 280                 285             


Arg Gln Leu Thr Glu Gln Glu Arg Gln Leu Cys Leu Pro Leu Pro Tyr 
    290                 295                 300                 


Arg Gln Ala Gly Asp Leu Thr Tyr Lys Gln Leu Gly Ser Ala Leu Ala 
305                 310                 315                 320 


Lys Ala Gly Leu Leu Glu Ala Gly Gly Phe Lys Phe Thr Gly Leu Ala 
                325                 330                 335     


Tyr Pro Thr Glu Ala Gln Lys Ala Glu Gly Lys Ala Lys Asp Pro Glu 
            340                 345                 350         


Ser Ala Thr Leu Val Lys Ile Pro Ala Trp His Glu Leu Arg Gln Thr 
        355                 360                 365             


Leu Thr Lys Gly Gly Leu Asn Asp Glu Trp Glu Lys Leu Ala Gly Asp 
    370                 375                 380                 


Ala Leu Cys Gly Gln Pro Glu Ile Phe Asp Lys Ile Ala Trp Val Leu 
385                 390                 395                 400 


Ser Val Tyr Lys Asp Asp Asp Glu Val Arg Ala Glu Leu Ala Lys Leu 
                405                 410                 415     


Ala Leu Pro Asn Pro Gln Ala Leu Thr Glu Ala Leu Leu Asn Ile Arg 
            420                 425                 430         


Phe Asp Lys Phe His Ser Leu Ser Leu Lys Ala Leu Arg Ala Ile Val 
        435                 440                 445             


Pro His Met Glu Gln Gly Leu Arg Tyr Asp Glu Ala Cys Val Gln Ala 
    450                 455                 460                 


Gly Tyr His His Ser Gln Leu Leu Lys Val Gly Glu Gly Glu His Lys 
465                 470                 475                 480 


Tyr Leu Pro Pro Leu Tyr Asp Gly Arg Asp Gly Asp Gly Arg Met Ile 
                485                 490                 495     


Phe Ala Glu Asp Ala Asp Ile Pro Arg Asn Pro Val Val Leu Arg Ser 
            500                 505                 510         


Leu Asn Gln Ala Arg Lys Val Val Asn Ala Ile Ile Arg Arg Tyr Gly 
        515                 520                 525             


Ser Pro Ser Ala Val His Ile Glu Met Ala Arg Asp Leu Ser Arg Pro 
    530                 535                 540                 


Phe Asp Glu Arg Asn Lys Ile Lys Lys Asp Gln Asp Glu Tyr Gln Ala 
545                 550                 555                 560 


Arg Asn Gln Arg Asp Arg Glu Gln Phe Ser Glu Leu Phe Gly His Pro 
                565                 570                 575     


Pro Lys Gly Leu Glu Phe Glu Lys Trp Arg Leu Tyr Arg Glu Gln Asn 
            580                 585                 590         


Gly Gln Cys Ala Tyr Ser Gln Arg Pro Leu Thr Ala Ser Gly Asn Val 
        595                 600                 605             


Ala Glu Ile Phe Leu Asp Gly Ser Thr Glu Ile Asp His Ala Leu Pro 
    610                 615                 620                 


Tyr Ser Arg Ser Phe Asp Asp Ser Lys Asn Asn Lys Val Leu Val Leu 
625                 630                 635                 640 


Thr Arg Glu Asn Arg Asp Lys Gly Asn Arg Thr Pro Tyr Glu Tyr Leu 
                645                 650                 655     


Gly Gly Ala Glu Asn Ser Glu Ser Trp Gln Arg Phe Val Ser Phe Val 
            660                 665                 670         


Glu Ser Asn Lys Ala Tyr Arg Leu Ala Lys Arg Thr Arg Leu Leu Arg 
        675                 680                 685             


Lys Asp Phe Gly Ala Lys Glu Ser Glu Glu Phe Arg Glu Arg Asn Leu 
    690                 695                 700                 


Asn Asp Thr Arg Tyr Val Cys Arg Phe Phe Lys Asn Tyr Val Glu Gln 
705                 710                 715                 720 


His Leu Lys Leu Ala Glu Gly Ser Glu Thr Lys Arg Cys Val Val Val 
                725                 730                 735     


Ser Gly Gln Leu Thr Asn Phe Leu Arg Ala Arg Trp Gly Leu Leu Lys 
            740                 745                 750         


Val Arg Ser Asp Ser Asp Arg His His Ala Leu Asp Ala Ala Val Val 
        755                 760                 765             


Ala Ala Cys Ser His Ser Met Val Gln Arg Leu Ser Asn Tyr Ser Arg 
    770                 775                 780                 


Thr Arg Glu Leu Glu Gln Val Arg Asp Gly Phe Val Asp Ile Glu Thr 
785                 790                 795                 800 


Gly Glu Ile Val Asn Pro Ala Met His Gln Gln Leu Arg Glu His Phe 
                805                 810                 815     


Pro Asp Pro Trp Pro Tyr Phe Arg His Glu Leu Glu Ala Arg Leu Lys 
            820                 825                 830         


Ile Asp Ser Pro Glu Leu Leu Arg Glu Glu Met Ala Arg Leu Gly Ser 
        835                 840                 845             


Tyr Ser Glu Glu Glu Leu Lys Val Leu Lys Pro Leu Phe Val Ser Arg 
    850                 855                 860                 


Ala Pro Gln Arg Arg Asn Gly Gly Ala Ala His Lys Asp Thr Ile Tyr 
865                 870                 875                 880 


Gly Gln Pro Glu Lys Leu Lys Lys Asn Gly Ser Val Thr Gln Lys Val 
                885                 890                 895     


Ala Leu Thr Ser Leu Thr Leu Lys Asp Ile Asp Leu Leu Ala Asp Pro 
            900                 905                 910         


His Arg Asn Ala Lys Leu Tyr Gly Ala Ile Arg Glu Arg Leu Glu Ala 
        915                 920                 925             


His Gly Gly Lys Gly Asp Lys Ala Phe Pro Ala Thr Asn Pro Leu Arg 
    930                 935                 940                 


Lys Pro Asp Lys Glu Gly Asn Pro Thr Gly Pro Ile Val Arg Thr Val 
945                 950                 955                 960 


Thr Lys Val Ile Asp Lys Leu Ser Gly Ile Pro Val Arg Gly Gly Ile 
                965                 970                 975     


Ala Lys Asn Asp Thr Met Leu Arg Val Asp Val Phe Thr Lys Gly Gly 
            980                 985                 990         


Lys Phe His Leu Val Pro Val Tyr  Val His His Arg Val  Thr Gly Leu 
        995                 1000                 1005             


Pro Asn  Arg Ala Ile Ile Ala  Phe Lys Asp Glu Asp  Glu Trp Thr 
    1010                 1015                 1020             


Leu Ile  Asp Asp Ser Phe Asp  Trp Cys Phe Ser Leu  Tyr Pro Asn 
    1025                 1030                 1035             


Asp Leu  Leu Arg Ile Lys Leu  Lys Lys Glu Ser His  Ser Gly Tyr 
    1040                 1045                 1050             


Tyr Ser  Ser Cys Asn Arg Ser  Thr Gly Ala Val Asn  Leu Trp Ala 
    1055                 1060                 1065             


His Asp  Arg Asn Val Asn Val  Gly Lys Asp Gly Phe  Ile Glu Ser 
    1070                 1075                 1080             


Ile Gly  Val Lys Met Ala Val  Ser Ile Glu Lys Leu  His Val Asp 
    1085                 1090                 1095             


Thr Leu  Gly Asn Ile Tyr Pro  Ala Pro Pro Glu Lys  Arg Arg Gly 
    1100                 1105                 1110             


Leu Ala  
    1115 


<210>  658
<211>  1116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  658

Met Ser Gln Asn Gln Asn Pro Asn Thr Gln Ser His Leu Asn Pro Thr 
1               5                   10                  15      


Thr Gln Ser Ser Thr Gln Ser Asn Pro Gln Pro Thr Thr Gln Glu Ser 
            20                  25                  30          


Leu Lys Ile Leu Gly Phe Asp Ile Gly Val Ala Ser Ile Gly Trp Ala 
        35                  40                  45              


Phe Val Glu Gly Gly Glu Leu Lys Asp Cys Gly Val Arg Ile Phe Thr 
    50                  55                  60                  


Lys Ala Glu Asn Pro Lys Asp Gly Ser Ser Leu Ala Leu Pro Arg Arg 
65                  70                  75                  80  


Glu Ala Arg Gly Val Arg Arg Arg Leu Ala Arg Arg Lys Gly Arg Leu 
                85                  90                  95      


Asn Ala Leu Lys Val Leu Ile Cys Lys Glu Phe Gly Leu Asn Arg Ser 
            100                 105                 110         


Asp Tyr Leu Ala Thr Ala Gly Glu Leu Pro Lys Ala Tyr Ala Thr Ser 
        115                 120                 125             


Lys Glu Thr Lys Ser Pro Tyr Gln Leu Arg Ala Glu Ser Leu Glu Arg 
    130                 135                 140                 


Lys Leu Ser Ala Asp Glu Phe Ala Arg Val Ile Leu His Ile Ala Lys 
145                 150                 155                 160 


His Arg Gly Tyr Gly Asn Lys His Ala Lys Glu Ser Ser Asp Asp Asp 
                165                 170                 175     


Ser Gly Lys Val Lys Lys Ala Ile Ser Glu Asn Gln Lys Val Met Ser 
            180                 185                 190         


Glu Lys Gly Tyr Thr Thr Ala Gly Gln Tyr Leu Tyr Gly Glu Phe Tyr 
        195                 200                 205             


Gln Lys Ala Arg Asn Phe Ser Glu Ala Glu Pro Lys Ser Pro Lys Asn 
    210                 215                 220                 


Ala Arg Gly Thr Gln Glu Phe Lys Asn Val Arg Asn Lys Ser Glu Asn 
225                 230                 235                 240 


Tyr Ala Arg Cys Leu Ala Gln Ser Glu Leu Gln Lys Glu Leu Glu Ile 
                245                 250                 255     


Ile Phe Ala Lys Gln Arg Glu Phe Gly Phe Gln Phe Ser Asp Lys Lys 
            260                 265                 270         


His Lys Thr Ile Asn Ala Asn Gly Lys Leu Lys Glu Leu Asp Phe Ala 
        275                 280                 285             


Asp Ala Val Leu Glu Ile Ala Phe Leu Gln Arg Pro Leu Lys Ser Phe 
    290                 295                 300                 


Ala Asp Lys Val Gly Lys Cys Thr Phe Tyr Glu Asp Lys Pro Arg Ala 
305                 310                 315                 320 


Pro Lys Asp Ser Leu Ser Ala Ile Glu Phe Val Ala Leu Thr Arg Ile 
                325                 330                 335     


Ile Asn Thr Leu Ala Asn Ile Thr Lys Arg Ser Gly Glu Val Tyr Ser 
            340                 345                 350         


Ala Asp Lys Val Arg Glu Ile Leu Ser Ile Val Leu Asp Lys Gly Glu 
        355                 360                 365             


Ile Thr Tyr Lys Asn Leu Arg Lys Val Ile Ala Leu Asp Glu Arg Leu 
    370                 375                 380                 


Gln Phe Pro Lys Asp Ser Lys Leu Asp Tyr Ala Lys Glu Asp Ala Glu 
385                 390                 395                 400 


Lys Ala Lys Ala Ile Glu Phe Ser Lys Leu Lys Ala Phe Arg Lys Ala 
                405                 410                 415     


Leu Gly Gly Ser Phe Glu Gly Phe Ile Arg Ala Glu Leu Asp Ser Ile 
            420                 425                 430         


Ala Thr Asp Ile Ala Leu Ile Lys Ser Lys Asp Glu Leu Lys Ala Lys 
        435                 440                 445             


Leu Ala Asn Tyr Gln Ser Leu Asn Asp Glu Gln Arg Glu Lys Leu Ser 
    450                 455                 460                 


Glu Leu Ser Phe Asp Lys Phe Ile Asp Leu Ser Phe Lys Ala Leu Glu 
465                 470                 475                 480 


Ala Ile Leu Pro Leu Met Arg Gly Asp Lys Asp Asp Lys Ile Tyr Arg 
                485                 490                 495     


Tyr Asp Glu Ala Trp Arg Glu Ala Gly Leu Val Glu Val Ala Lys Lys 
            500                 505                 510         


Thr Gln Lys Gly Asp Ser Leu Pro Pro Leu Ile Glu Tyr Glu Lys Asp 
        515                 520                 525             


Leu Ala Asn Pro Val Val Ala Arg Ala Leu Ala Glu Tyr Arg Lys Val 
    530                 535                 540                 


Leu Asn Ala Leu Leu Lys Lys His Gly Lys Val His Lys Ile His Leu 
545                 550                 555                 560 


Glu Phe Thr Arg Gln Ala Gly Val Ser Ser Lys Glu Arg Gly Glu Ile 
                565                 570                 575     


Ile Ala Glu Gln Asn Lys Arg Leu Lys Ala Asn Glu Glu Ala Arg Lys 
            580                 585                 590         


Arg Cys Glu Glu Tyr Gly Leu Ile Pro Ser Gly Lys Asn Ile Leu Lys 
        595                 600                 605             


Leu Lys Leu Trp Gln Glu Gln Gly Glu Phe Cys Val Tyr Ser Gly Lys 
    610                 615                 620                 


Lys Ile Thr Gln Ser Asp Leu Arg Asp Glu Ser Thr Leu Gln Ile Asp 
625                 630                 635                 640 


His Ile Tyr Pro Tyr Ser Arg Ser Tyr Asp Asp Ser Gln Asn Asn Lys 
                645                 650                 655     


Val Leu Val Phe Thr Asp Ala Asn Gln Asn Lys Leu Asn Gln Thr Pro 
            660                 665                 670         


Tyr Glu Ala Phe Gly Lys Asp Ile Ser Lys Trp Asn Gln Ile Leu Gly 
        675                 680                 685             


Arg Ile Glu Lys Leu Pro Lys Ala Lys Lys Asn Arg Ile Thr Asn Lys 
    690                 695                 700                 


Asn Phe Ala Asn Lys Glu Ala Gly Phe Ile Ala Arg Asn Ile Val Asp 
705                 710                 715                 720 


Thr Ser Tyr Ile Ala Arg Leu Ser Ala Ser Tyr Thr Asp Ala Phe Ile 
                725                 730                 735     


Glu Phe Cys Pro Leu Ala Glu Asn Glu Asp Thr Thr Leu Gly Arg Gly 
            740                 745                 750         


Glu Lys Gly Ser Lys Lys His Ile Ser Val Val Asn Gly Ser Leu Thr 
        755                 760                 765             


Ala Thr Met Arg His Tyr Trp Gly Leu Asn Thr Leu Leu Asp Gly Glu 
    770                 775                 780                 


Asn Ser Glu Ser Lys Asp Arg Ser Asn His Leu His His Ala Leu Asp 
785                 790                 795                 800 


Ala Ile Ile Ile Ala Tyr Val Asn Asp Ser Val Ile Lys Ala Phe Ser 
                805                 810                 815     


Asp Phe Lys Lys Thr Gln Glu Gln Asn Lys Ala Arg Leu Ile Ala Lys 
            820                 825                 830         


Glu Ile Glu Ser Ala Glu Tyr Lys Ile Ser Arg Arg Phe Phe Ser Pro 
        835                 840                 845             


Ser Gly Phe Glu Asn Asn Glu Ala Phe Arg Gln Ala Ile Lys Arg Lys 
    850                 855                 860                 


Ile Leu Gly Gln Ser Leu Glu Ser Arg Glu Asn Asp Met Thr Ile Asn 
865                 870                 875                 880 


Ser Glu Ala Leu Gly Glu Lys Val Gly Gly Ile Phe Val Ser Lys Pro 
                885                 890                 895     


Pro Arg Lys Arg Ala Arg Gly Ala Leu His Lys Glu Thr Phe Ser Ser 
            900                 905                 910         


Ile Asp Asp Lys Asp Leu Leu Lys Thr Tyr Gly Gly Lys Glu Gly Val 
        915                 920                 925             


Asn Arg Ala Ile Lys Leu Gly Lys Val Arg Gln Ile Gly Ser Lys Ile 
    930                 935                 940                 


Ala Asp Asn Gly Thr Met Val Arg Val Asp Ile Phe Arg His Lys Val 
945                 950                 955                 960 


Ser Gly Lys Phe Tyr Gly Val Pro Ile Tyr Thr Met Asp Phe Ala Leu 
                965                 970                 975     


Gly Ile Leu Pro Asn Lys Ala Val Val Gly Gly Lys Asp Lys Ser Gly 
            980                 985                 990         


Val Ile Lys Asp Trp Leu Pro Met  Asp Ser Asn Tyr Glu  Phe Cys Phe 
        995                 1000                 1005             


Ser Leu  Phe Lys Asp Asp Leu  Ile Leu Val Gln Lys  Lys Glu Met 
    1010                 1015                 1020             


Ser Lys  Ala Glu Leu Cys Tyr  Phe Ala Gly Phe Asp  Thr Ser Gly 
    1025                 1030                 1035             


Gly Gln  Ile Lys Val Glu Lys  His Asp Asn His Phe  Ala Thr Leu 
    1040                 1045                 1050             


Thr Asp  Asn Gln Arg Leu Leu  Phe Asn Ile Ala Thr  Pro Lys Glu 
    1055                 1060                 1065             


Val Ala  Gly Lys Ser Ile Gly  Ile Gln Asn Leu Lys  Val Phe Glu 
    1070                 1075                 1080             


Lys Trp  Gln Val Ser Pro Leu  Gly Glu Val Ser Lys  Ala Glu Phe 
    1085                 1090                 1095             


Lys Glu  Arg Glu Pro Ile Ala  Leu Lys Ser Thr Pro  Lys Arg Phe 
    1100                 1105                 1110             


Leu Gln  Gly 
    1115     


<210>  659
<211>  1117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  659

Met Lys His Ile Leu Gly Leu Asp Leu Gly Ser Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Val Gln Gln Asp Phe Glu Asn Lys Gln Gly Lys Ile Ile Ala 
            20                  25                  30          


Thr Gly Ser Arg Ile Ile Pro Met Asp Gln Gly Ile Leu Gly Asp Phe 
        35                  40                  45              


Glu Arg Gly Asn Thr Val Ser Gln Thr Ala Glu Arg Thr Thr Tyr Arg 
    50                  55                  60                  


Ser Met Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Leu Leu Gly Phe Leu Pro Pro His Tyr Asp Ala 
                85                  90                  95      


Gln Ile Asp Phe Thr Lys Arg Tyr Gly Lys Phe Ile Asp Asn Ala Glu 
            100                 105                 110         


Pro Lys Ile Ala Tyr Asn Asn Gly Asn Phe Ile Phe Met Asn Ser Phe 
        115                 120                 125             


Asn Glu Met Val Glu Asp Phe Lys Lys His Gln Pro Gln Leu Phe Tyr 
    130                 135                 140                 


Lys Lys Ser Asn Gly Glu Glu Ser Arg Ile Pro Tyr Asp Trp Thr Ile 
145                 150                 155                 160 


Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Lys Ile Thr Gln Gln Glu 
                165                 170                 175     


Leu Ala Trp Leu Ile Leu His Phe Asn Gln Lys Arg Gly Tyr Tyr Gln 
            180                 185                 190         


Leu Arg Gly Glu Glu Glu Thr Glu Asn Pro Asn Lys Glu Val Ala Phe 
        195                 200                 205             


His Ser Leu Lys Val Val Asp Val Glu Ala Glu Ala Pro Asn Lys Lys 
    210                 215                 220                 


Gly Glu Ile Trp Tyr Thr Ile Arg Leu Glu Asn Gly Trp Ile Tyr Arg 
225                 230                 235                 240 


Arg Thr Ser Lys Asn Pro Leu Asp Asp Trp Lys Gly Lys Thr Arg Asp 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Asn Asp Asp Gly Thr Ile Lys Leu Asp 
            260                 265                 270         


Lys Glu Gly Lys Glu Lys Arg Ser Phe Arg Ala Pro Asp Glu Asn Asp 
        275                 280                 285             


Trp Asn Leu Leu Lys Lys Lys Thr Glu His Asp Ile Asp Phe Ser Gly 
    290                 295                 300                 


Lys Pro Val Gly Thr Tyr Ile Tyr Asp His Leu Leu Gln Asn Pro Asn 
305                 310                 315                 320 


Gln Lys Ile Lys Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Asp Glu Leu Lys Ala Ile Leu Ala Lys Gln Ile Ala Leu Gln Pro 
            340                 345                 350         


Glu Leu Phe Thr Glu Thr Leu Leu Ala Asp Cys Ile Arg Glu Leu Tyr 
        355                 360                 365             


Arg Lys Asn Glu Thr Gln Gln Gln Asn Leu Leu Ser Lys Asp Phe Leu 
    370                 375                 380                 


His Leu Phe Val Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Cys Ser 
385                 390                 395                 400 


Lys Lys Ser Thr Ile Ala Asn Cys Thr Leu Glu Lys Arg Ser Tyr Ile 
                405                 410                 415     


Asp Lys Glu Thr Asn Glu Arg Lys Asp Ala Pro Ile Lys Val Cys Ala 
            420                 425                 430         


Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Val Leu Gln Trp Leu Gln 
        435                 440                 445             


Asn Leu Arg Ile Tyr Glu Ile Glu Thr Asp Arg Glu Val Thr Ala Glu 
    450                 455                 460                 


Phe Ile Glu Thr Pro Gln Asp Tyr Glu Ala Leu Phe Asn Phe Leu Met 
465                 470                 475                 480 


Ser Gln Lys Glu Ile Asp Asn Glu Gly Leu Leu Lys Tyr Phe Leu Phe 
                485                 490                 495     


Ala Lys Asn Pro Gln Ile Lys Asp Lys Ala Ala Lys Ser Glu Leu Lys 
            500                 505                 510         


Lys Trp Leu Thr Thr Tyr Arg Trp Asn Tyr Val Tyr Asp Ala Ala Asp 
        515                 520                 525             


Asn Ser Ser Lys Lys Tyr Pro Met Asn Glu Thr Gly Tyr Glu Leu Gln 
    530                 535                 540                 


Arg Tyr Leu Gln Lys Val Glu Gly Leu Pro Ala Asn Phe Leu Thr Phe 
545                 550                 555                 560 


Asp Ile Thr Tyr Gln Leu Trp His Leu Leu Tyr Ser Val Lys Asp Lys 
                565                 570                 575     


Val Glu Phe Glu Lys Ala Leu Lys Lys Phe Ala Lys Lys Lys Gln Leu 
            580                 585                 590         


Asp Lys Thr Ser Phe Val Asp Ser Phe Lys Asn Phe Lys Pro Tyr Pro 
        595                 600                 605             


Asn Glu Tyr Gly Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro 
    610                 615                 620                 


Leu Met Arg Phe Gly Asn His Trp Asp Phe Asn Arg Ile Asp Val Ser 
625                 630                 635                 640 


Thr Gln Lys Arg Ile Asp Asp Leu Ile Asn Gly Val Ala Asn Glu Asp 
                645                 650                 655     


Ile Arg Thr Ile Ile Arg Glu Lys Ala Glu Lys Tyr Gln Leu Asn Ser 
            660                 665                 670         


Glu Thr Cys Phe Gln Asp Leu Pro Leu Trp Leu Ala Gln Tyr Val Val 
        675                 680                 685             


Tyr Asn Arg His Ala Glu Ala Thr Asp Ile Glu Lys Trp Thr Ser Val 
    690                 695                 700                 


Ser Asp Leu Glu Ala Tyr Leu Glu Asp Phe Lys Gln His Ser Leu Arg 
705                 710                 715                 720 


Asn Pro Ile Val Glu Gln Val Val Thr Glu Thr Leu Arg Val Val Arg 
                725                 730                 735     


Asp Ile Trp Gln Gln Tyr Ser Gly Gly Gln Ala Asn Phe Phe Asp Glu 
            740                 745                 750         


Ile His Ile Glu Leu Gly Arg Glu Leu Lys Lys Thr Ala Glu Glu Arg 
        755                 760                 765             


Lys Gln Leu Ser Asp Lys Asn Gln Glu Asn Glu Asn Thr Asn Leu Arg 
    770                 775                 780                 


Ile Lys Lys Leu Leu Ala Glu Leu Lys Glu Asp Ala Ser Ile Gln Asn 
785                 790                 795                 800 


Val Arg Pro Tyr Ser Leu Val Gln Gln Glu Leu Leu Lys Ile Tyr Glu 
                805                 810                 815     


Glu Gly Val Phe Ser Ser Thr Asp Glu Ile Glu Glu Asp Ile Gln Lys 
            820                 825                 830         


Ile Arg Lys Lys Val Glu Pro Thr Pro Pro Glu Ile Gln Arg Tyr Lys 
        835                 840                 845             


Leu Trp Leu Glu Gln Lys Tyr Lys Ser Pro Tyr Thr Gly Glu Val Ile 
    850                 855                 860                 


Ser Leu Thr Lys Leu Phe Thr Glu Glu Tyr Glu Ile Glu His Ile Ile 
865                 870                 875                 880 


Pro Gln Ser Arg Tyr Tyr Asp Asp Ser Leu Ser Asn Lys Val Ile Cys 
                885                 890                 895     


Glu Ser Ala Val Asn Lys Leu Lys Thr Asn Gln Leu Gly Leu Glu Phe 
            900                 905                 910         


Ile Gln Asn His His Gly Glu Lys Val Arg Ile Thr Ala Asn Lys Glu 
        915                 920                 925             


Val Thr Ile Phe Thr Glu Glu Gln Tyr Lys Glu Phe Val Lys Lys His 
    930                 935                 940                 


Tyr Ala Asn Asn Ser Ala Lys Gln Gln Lys Leu Leu Leu Asn Glu Ile 
945                 950                 955                 960 


Pro Glu Lys Met Val Ala Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser 
                965                 970                 975     


Lys Cys Ile Ser Glu Ile Leu Ser Lys Ile Val Arg Ser Asp Glu Lys 
            980                 985                 990         


Asp Glu Gly Val Asn Ser Lys Asn  Ile Ile Pro Cys Thr  Gly Lys Ile 
        995                 1000                 1005             


Thr Thr  Ala Leu Lys Gln Asp  Trp Gly Leu Asn Asp  Val Trp Asn 
    1010                 1015                 1020             


Glu Leu  Ile Leu Pro Arg Phe  Glu Arg Met Asn Gln  Leu Thr Gln 
    1025                 1030                 1035             


Thr Gln  Leu Phe Thr Ser Tyr  Asn Asp Arg Leu Gln  Lys Asn Leu 
    1040                 1045                 1050             


Pro Thr  Val Pro Ile Glu Tyr  Ser Gln Gly Phe Gln  Lys Lys Arg 
    1055                 1060                 1065             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1070                 1075                 1080             


Ala Thr  Arg Glu His Ile Asn  Tyr Met Asn Asn Gln  His Ala Leu 
    1085                 1090                 1095             


Asp Lys  Ser Lys Asp Lys Lys  Glu Lys Gln Gln Ser  Arg Ile Asp 
    1100                 1105                 1110             


Leu His  Pro Leu 
    1115         


<210>  660
<211>  1117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  660

Met Gln Thr Lys Asn Leu Leu Tyr Thr Leu Gly Leu Asp Ile Gly Ile 
1               5                   10                  15      


Ala Ser Val Gly Trp Cys Val Leu Gly Glu Asn Arg Ile Ile Asp Leu 
            20                  25                  30          


Gly Val Arg Cys Phe Asp Lys Ala Glu Thr Pro Asp Arg Gly Glu Ser 
        35                  40                  45              


Leu Asn Lys Val Arg Arg Asp Ala Arg Leu Met Arg Arg Arg Leu Arg 
    50                  55                  60                  


Arg Arg Ala Trp Arg Leu Thr Lys Leu Ala Arg Leu Leu Lys His Ala 
65                  70                  75                  80  


Gly Leu Ile Asp Ser Thr Arg Ile Val Arg Asp Arg His Asp Gly Ala 
                85                  90                  95      


Val Ser Pro Trp Gln Leu Arg Val Glu Gly Leu Asp Arg Lys Leu Ala 
            100                 105                 110         


Pro Glu Glu Trp Ala Arg Val Ile Tyr His Ile Val Lys His Arg Gly 
        115                 120                 125             


Phe His Trp Ile Ser Arg Ala Glu Glu Arg Lys Ala Glu Ser Asp Lys 
    130                 135                 140                 


Gly Glu Gly Gly Lys Val Lys Gln Gly Leu Ser Ala Thr Lys Gln Leu 
145                 150                 155                 160 


Met Val Glu Lys Asn Tyr Arg Ser Ala Ala Glu Met Val Leu Thr Glu 
                165                 170                 175     


Phe Pro Gly Ala Gln Arg Asn Lys Arg Gly Glu Tyr Thr Lys Ala Leu 
            180                 185                 190         


Ser Arg Ile Leu Leu Ala Ala Glu Leu Thr Thr Leu Phe Glu Arg Gln 
        195                 200                 205             


Gln Glu Leu Gly Asn Pro His Ala Gly Pro Asp Leu Gln Thr Lys Ile 
    210                 215                 220                 


Leu Gly Asn Gly Asp Arg Lys Ser Gly Leu Leu Trp Met Gln Lys Pro 
225                 230                 235                 240 


Pro Leu Ser Gly Ser Ala Leu Leu Lys Met Leu Gly Lys Cys Thr Phe 
                245                 250                 255     


Glu Arg Glu Glu Tyr Arg Ala Pro Lys Ala Ser Phe Thr Ala Glu Arg 
            260                 265                 270         


His Val Trp Leu Thr Lys Leu Asn Asn Leu Arg Val Leu Val Asp Gly 
        275                 280                 285             


Val Thr Arg Pro Leu Ser Asp Pro Glu Arg Arg Leu Ala Leu Ser Leu 
    290                 295                 300                 


Pro Tyr Gln Gln Ala Ser Asp Phe Thr Tyr Lys Gln Leu Arg Ala Ala 
305                 310                 315                 320 


Leu Cys Lys Asn Gly Leu Leu Pro Asp Ser Phe Gln Phe Ser Gly Leu 
                325                 330                 335     


Ala Tyr Pro Thr Gly Ala Gln Gln Pro Asp Gly Lys Ala Lys Asn Pro 
            340                 345                 350         


Glu Asn Asp Arg Leu Val Lys Leu Pro Ala Trp Gln Glu Phe Arg Lys 
        355                 360                 365             


Thr Leu Arg Asp Ala Gly Leu Glu Thr Glu Trp Glu Ser Ile Ala Gly 
    370                 375                 380                 


Met Ala Ala Gly Gly Gln Pro Asp Leu Leu Asp Gln Ile Gly Trp Val 
385                 390                 395                 400 


Leu Ser Val Phe Lys Asp Gly Asp Glu Val Leu Arg Glu Leu Arg Lys 
                405                 410                 415     


Ile Pro Leu Pro Gly Gly Glu Lys Met Val Gln Ala Leu Ser Gly Val 
            420                 425                 430         


Ser Phe Asp Thr Phe His Asn Leu Ser Leu Lys Ala Leu Arg Lys Ile 
        435                 440                 445             


Val Pro His Met Glu Ala Gly Leu Arg Tyr Asp Glu Ala Cys Leu Ala 
    450                 455                 460                 


Ala Gly Tyr His His Ser Asp Leu Arg Lys Asp Thr Asp Glu Arg Ser 
465                 470                 475                 480 


Leu Tyr Leu Pro Pro Leu Tyr Ser Gly Arg Thr Thr Asp Gly Arg Met 
                485                 490                 495     


Val Phe Arg Asp Asp Ile Asp Val Pro Arg Asn Pro Val Val Leu Arg 
            500                 505                 510         


Ala Ile Asn Gln Ala Arg Thr Val Val Asn Ser Val Val Arg Arg Tyr 
        515                 520                 525             


Gly Ser Pro Ser Ser Met His Ile Glu Met Ala Arg Asp Leu Ser Arg 
    530                 535                 540                 


Pro Trp Asp Glu Arg Asn Lys Val Lys Arg Ala Gln Glu Glu Tyr Arg 
545                 550                 555                 560 


Asp Arg Asn Glu Lys Leu Lys Ile Asp Phe Ala Ala Glu Tyr Gly Ile 
                565                 570                 575     


Glu Pro Lys Ser Arg Glu Phe Glu Lys Phe Leu Leu Tyr Arg Glu Gln 
            580                 585                 590         


Gly Gly Lys Cys Ala Tyr Ser Leu Lys Pro Leu Asp Leu Asp Arg Val 
        595                 600                 605             


Leu Arg Asp Leu Gly Tyr Ala Glu Val Asp His Ala Leu Pro Tyr Ser 
    610                 615                 620                 


Arg Ser Phe Asp Asp Ser Lys Asn Asn Lys Val Leu Val Leu Ala Glu 
625                 630                 635                 640 


Glu Asn Arg Asn Lys Gly Asn Arg Thr Pro Tyr Glu Tyr Leu Asp Gly 
                645                 650                 655     


Thr Ser Glu Ser Pro Arg Trp Arg Glu Phe Lys Ala Phe Val Glu Thr 
            660                 665                 670         


Asn Lys Ala Tyr Arg Leu Ala Lys Arg Ala Arg Leu Leu Arg Lys Asp 
        675                 680                 685             


Phe Ser Gly Lys Gly Ser Glu Glu Phe Arg Asp Arg Asn Leu Asn Asp 
    690                 695                 700                 


Thr Arg Tyr Ile Ser Arg Phe Phe Lys Asn Tyr Val Glu Arg Phe Leu 
705                 710                 715                 720 


Lys Leu Ala Glu Glu Ser Glu Glu Arg Thr Cys Val Val Leu Ser Gly 
                725                 730                 735     


Gln Leu Thr Ala Phe Leu Arg Ala Arg Trp Gly Leu Ala Lys Val Arg 
            740                 745                 750         


Gly Asp Ser Asp Arg His His Ala Leu Asp Ala Ala Val Ile Ala Ala 
        755                 760                 765             


Cys Ser Arg Ser Met Val Lys Arg Leu Ser Asp Tyr Ala Arg Arg His 
    770                 775                 780                 


Glu Leu Glu Lys Val Arg Thr Gly Phe Val Asp Phe Glu Thr Gly Glu 
785                 790                 795                 800 


Ile Val Asp Ala Gly Lys Tyr Arg Gln Ile Glu Arg Asp Phe Pro Ser 
                805                 810                 815     


Pro Trp Pro Tyr Phe Arg Asp Glu Leu Leu Thr Arg Leu Asn Thr Asp 
            820                 825                 830         


Asp Pro Ala Glu Leu Arg Glu Arg Met Gln Ser Leu Gly Ser Tyr Pro 
        835                 840                 845             


Pro Glu Ala Leu Glu Thr Leu Arg Pro Met Phe Val Ser Arg Ala Pro 
    850                 855                 860                 


Gln Arg Arg Asn Gly Gly Ala Ala His Lys Asp Thr Ile Tyr Arg Gln 
865                 870                 875                 880 


Thr Glu Arg Leu Lys Lys Val Gly Ser Val Thr Gln Lys Val Pro Leu 
                885                 890                 895     


Ala Ala Leu Asn Leu Arg Asp Leu Glu Asn Leu Val Asp Pro His Arg 
            900                 905                 910         


Asn Glu Lys Leu Tyr Ala Ala Ile Arg Ala Arg Leu Glu Ala His Gly 
        915                 920                 925             


Gly Lys Gly Glu Lys Ala Phe Pro Pro Asn Asn Pro Leu Arg Lys Pro 
    930                 935                 940                 


Asp Arg Asp Gly Asn Pro Thr Gly Pro Ile Val Arg Thr Val Thr Leu 
945                 950                 955                 960 


Val Ile Asp Lys Met Ser Gly Ile Pro Val Arg Gly Gly Val Ala Lys 
                965                 970                 975     


Asn Asp Thr Met Leu Arg Val Asp Ile Phe Arg Ser Lys Lys Asp Gly 
            980                 985                 990         


Lys Phe His Leu Val Pro Leu Tyr  Val His His Ala Val  Ala Lys Gln 
        995                 1000                 1005             


Leu Pro  Thr Arg Ala Val Ile  Ala Tyr Lys Asp Glu  Gly Glu Trp 
    1010                 1015                 1020             


Thr Pro  Ile Asn Asn His Asp  Phe Asp Phe Leu Phe  Ala Leu Tyr 
    1025                 1030                 1035             


Pro Asn  Asp Leu Val Arg Ile  Thr Leu Lys Lys Glu  Arg His Phe 
    1040                 1045                 1050             


Gly Tyr  Tyr Ala Gly Cys Asn  Arg Ala Thr Gly Ala  Val Ser Ile 
    1055                 1060                 1065             


Trp Ala  His Asp Arg Asn Gln  Arg Ile Gly Lys Asp  Gly Phe Ile 
    1070                 1075                 1080             


Asp Gly  Ile Gly Val Lys Thr  Ala Leu Ser Val Glu  Lys Phe His 
    1085                 1090                 1095             


Val Asp  Thr Leu Gly Asn Val  Tyr Pro Ala Pro Pro  Glu Thr Arg 
    1100                 1105                 1110             


Arg Gly  Leu Ala 
    1115         


<210>  661
<211>  1118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  661

Met Gly Thr Arg Tyr Ser Phe Asp Ile Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Trp Arg Thr Gly Arg Asp Pro Lys Gly Ile Phe Gly Glu Lys 
            20                  25                  30          


Asp Ala Pro Leu Glu Leu Leu Gly Ala Gly Val Arg Leu Phe Lys Asp 
        35                  40                  45              


Gly Arg Asn Pro Lys Asp Gly Gln Ser Leu Ala Thr Met Arg Arg Ile 
    50                  55                  60                  


Pro Arg Gln Ala Arg Lys Arg Arg Asp Arg Phe Val Leu Arg Arg Glu 
65                  70                  75                  80  


Ala Leu Val Ala Ala Leu Ile Glu Ala Gly Leu Met Pro Ala Ser Glu 
                85                  90                  95      


Lys Glu Arg Lys Ala Leu Ala Asp Leu Asp Pro Tyr Gln Leu Arg Ala 
            100                 105                 110         


Ala Gly Leu Asp Lys Ala Leu Ala Pro His Glu Leu Gly Arg Ala Ile 
        115                 120                 125             


Phe His Leu His Gln Arg Arg Gly Phe Lys Ser Asn Arg Lys Thr Asp 
    130                 135                 140                 


Arg Lys Ser Asp Asp Arg Asp Lys Gly Lys Ile Ala Thr Ala Ser Ala 
145                 150                 155                 160 


Ala Leu Ala Lys Lys Phe Glu Asp Glu Asn Ala Arg Thr Phe Gly Glu 
                165                 170                 175     


Phe Leu Trp Arg Arg His Arg Asp Pro Asp Met Lys Arg Gln Ala Val 
            180                 185                 190         


Leu Lys Ala His Asp Pro Ala Lys Gly Lys Pro Asp Leu Arg Ala Ala 
        195                 200                 205             


Lys Leu Arg Gln Pro Thr Arg Ile Arg Leu Glu Gly Gln Gly Ala Thr 
    210                 215                 220                 


Ala Leu Tyr Glu Phe Tyr Pro Thr Arg Glu Met Leu Cys His Glu Phe 
225                 230                 235                 240 


Asp Ala Leu Trp Glu Ala Gln Ala Arg His His Pro Met Leu Leu Thr 
                245                 250                 255     


Arg Glu Ala Gly Asp Lys Ile Arg Ser Ile Leu Phe Arg Gln Arg Glu 
            260                 265                 270         


Leu Lys Pro Pro Leu Ile Gly Leu Cys Thr Phe Glu Tyr Ala Ala Gly 
        275                 280                 285             


Glu Lys Glu Ser Arg Leu Pro Lys Ala Leu Pro Ser Val Gln Ala Arg 
    290                 295                 300                 


Glu Ile Tyr Glu Arg Leu Gly His Ile Arg Leu Ser Thr Gly Ser Gly 
305                 310                 315                 320 


Thr Asp Arg Pro Leu Lys Ser Ser Glu Arg Asp Asp Leu Ala Thr Ala 
                325                 330                 335     


Leu Leu His Cys Gly Leu Thr Lys Ile Lys Val Ser Lys Thr Lys Gly 
            340                 345                 350         


Leu Ala Phe Lys Thr Glu Phe Arg Lys Ala Leu Arg Leu Gly Gly Glu 
        355                 360                 365             


Val Arg Ile Asn Phe Glu Glu Met Gly Glu Glu Gly Leu Val Asp Leu 
    370                 375                 380                 


Arg Thr Asn Arg Leu Leu Val Gln Asp Asp His Tyr Gly Pro Arg Trp 
385                 390                 395                 400 


Arg Ala Leu Ser Trp Ser Gly Lys Asp Ala Phe Val Lys Lys Leu Leu 
                405                 410                 415     


Glu Ala Thr Asp Asp Asp Lys Leu Val Ala Arg Leu Met Thr Glu Asp 
            420                 425                 430         


Gly Leu Ser Ala Val Gln Ala Arg Asn Ala Ala Ser Ile Pro Leu Pro 
        435                 440                 445             


Asp Gly Tyr Ser Arg Leu Gly Pro Thr Ala Asn Ala Ala Ile Leu Gln 
    450                 455                 460                 


Ala Leu Ile Asp Ser Arg Asp Glu Asn Gly Phe Val Val Thr Tyr Asp 
465                 470                 475                 480 


Lys Ala Val Arg Arg Ala Gly Glu Arg Leu Gly Lys Asp Trp His His 
                485                 490                 495     


Ser Asp Glu Arg Asp Gly Glu Ile Leu Asp Ala Leu Pro Tyr Tyr Gly 
            500                 505                 510         


Lys Val Leu Gln Arg His Ile Leu Pro Gly Met Met Asp Pro Ala Asp 
        515                 520                 525             


Arg Lys Asp Glu Ala Lys Phe Tyr Gly Arg Ile Met Asn Pro Ser Val 
    530                 535                 540                 


His Ile Gly Leu Asn Gln Leu Arg Arg Leu Val Asn Ala Leu Ile Glu 
545                 550                 555                 560 


Arg Phe Gly Pro Pro Asp Gln Ile Val Val Glu Leu Ala Arg Asp Leu 
                565                 570                 575     


Lys Met Ser Ala Arg Glu Lys Glu Gln Glu Gln Arg Arg Asn Arg Asp 
            580                 585                 590         


Asn Arg Glu Ala Asn Glu Lys Arg Thr Lys Lys Leu Val Glu Leu Gly 
        595                 600                 605             


Gln Asp Asn Asn Gly Glu Asn Arg Ala Arg Leu Lys Leu Tyr Asp Glu 
    610                 615                 620                 


Gln Gln Arg Ala Gly Asn Gly Val Ala His Cys Pro Phe Thr Leu Asp 
625                 630                 635                 640 


Pro Ile Gly Ile Thr Gln Leu Phe Ser Pro Glu Ile Glu Ile Glu His 
                645                 650                 655     


Val Leu Pro Arg Ser Arg Thr Leu Asp Asp Ser Ala Ala Asn Lys Val 
            660                 665                 670         


Leu Cys Phe Arg Thr Ile Asn Arg Ile Lys Arg Gly Lys Thr Pro Trp 
        675                 680                 685             


Glu Ala Phe His Ala Asp Pro Lys Trp Pro Asp Ile Ala Ala Asn Ala 
    690                 695                 700                 


Glu Lys Leu Pro Pro Asn Lys Arg Trp Arg Phe Arg Glu Asp Ala Met 
705                 710                 715                 720 


Asp Val Phe Glu Ala Arg Thr Ser Gly Lys Met Asp Lys Thr Thr Leu 
                725                 730                 735     


Glu Gln Met Gly Leu Thr Thr Gly Phe Gln Ala Arg His Leu Asn Glu 
            740                 745                 750         


Thr Lys His Met Ser Arg Leu Ala Lys Ala Tyr Leu Gly Lys Ile Cys 
        755                 760                 765             


Asp Pro Asp Ala Val Tyr Val Thr Pro Gly Ala Leu Thr Ser Leu Leu 
    770                 775                 780                 


Arg Gly Lys Trp Gly Leu Asn Ser Ile Leu Ser Asp Asp Asn Leu Lys 
785                 790                 795                 800 


Asp Arg Thr Asp His Arg His His Ala Ile Asp Ala Ile Thr Ile Gly 
                805                 810                 815     


Ala Met Thr Arg Ser Leu Leu Leu Glu Leu Asn Thr Leu Ala Gly Lys 
            820                 825                 830         


Ala Glu Gln Ser Asp Tyr Asp Asp Val Val Gly Lys Val Pro Trp Pro 
        835                 840                 845             


Phe Asp Asn Phe Arg Asp Ala Val Arg Ser Val Val Asp Arg Val Val 
    850                 855                 860                 


Val Ser Asn Lys Pro Glu His Gly Lys Gln Gly Ala Leu His Glu Asp 
865                 870                 875                 880 


Thr Ala Tyr Gly Ile Val Gly Asp Glu Ala Glu Ala Glu Glu Ile Gly 
                885                 890                 895     


Asn Leu Val Arg Arg Lys Leu Leu Val Asp Leu Thr Pro Gly Glu Val 
            900                 905                 910         


Asp Arg Ile Arg Asp Ala Gly Leu Arg Lys Ala Met Gln Ala Ala Val 
        915                 920                 925             


Ala Pro Ser Arg Asp Ala Arg Gly Lys Val Ser Asp Ala Lys Gly Phe 
    930                 935                 940                 


Ala Ala Ala Leu Asp Thr Phe Ala Lys Ala Gln Gly Met Arg Arg Ile 
945                 950                 955                 960 


Arg Val Gly Lys Glu Asp Met Ser Ala Val Thr Ile His Asp Arg Gln 
                965                 970                 975     


Thr Asp Lys Ala Tyr Lys Ala Val Ala Pro Gly Glu Asn His His Val 
            980                 985                 990         


Asp Val Val Gln Met Arg Asp Gly  Ala Trp Lys Gly Phe  Ala Val Thr 
        995                 1000                 1005             


Val Phe  Glu Ala Asn Gln Lys  Asp Phe Arg Pro Gln  Trp Glu Arg 
    1010                 1015                 1020             


Glu Lys  Leu Gly Gly Lys Leu  Val Met Arg Leu His  Lys Gly Asp 
    1025                 1030                 1035             


Ala Val  Glu Leu Glu Ser Ser  Gly Val Arg Leu Val  Lys Phe Val 
    1040                 1045                 1050             


Gln Gln  Ile Trp Met Lys Ser  Asn Leu Val Leu Leu  Thr Glu His 
    1055                 1060                 1065             


Asn Glu  Gly Gly Asp Leu Gln  Lys Arg His Gln Asp  Ala Asp Asp 
    1070                 1075                 1080             


Pro Phe  Arg Trp Asp Phe Ala  Asn Ile Gly Lys Leu  Lys Glu Arg 
    1085                 1090                 1095             


Gly Cys  Val Ala Val Lys Val  Asp Glu Leu Gly Arg  Val Thr Lys 
    1100                 1105                 1110             


Arg Ile  Ser Asn Thr 
    1115             


<210>  662
<211>  1119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  662

Met Gly Tyr Arg Ile Gly Leu Asp Val Gly Ile Thr Ser Thr Gly Tyr 
1               5                   10                  15      


Ala Val Leu Lys Thr Asp Lys Asn Gly Leu Pro Tyr Lys Ile Leu Thr 
            20                  25                  30          


Leu Asp Ser Val Ile Tyr Pro Arg Ala Glu Asn Pro Gln Thr Gly Ala 
        35                  40                  45              


Ser Leu Ala Glu Pro Arg Arg Ile Lys Arg Gly Leu Arg Arg Arg Thr 
    50                  55                  60                  


Arg Arg Thr Lys Phe Arg Lys Gln Arg Thr Gln Gln Leu Phe Ile His 
65                  70                  75                  80  


Ser Gly Leu Leu Ser Lys Pro Glu Ile Glu Gln Ile Leu Ala Thr Pro 
                85                  90                  95      


Gln Ala Lys Tyr Ser Val Tyr Glu Leu Arg Val Ala Gly Leu Asp Arg 
            100                 105                 110         


Arg Leu Thr Asn Ser Glu Leu Phe Arg Val Leu Tyr Phe Phe Ile Gly 
        115                 120                 125             


His Arg Gly Phe Lys Ser Asn Arg Lys Ala Glu Leu Asn Pro Glu Asn 
    130                 135                 140                 


Glu Ala Asp Lys Lys Gln Met Gly Gln Leu Leu Asn Ser Ile Glu Glu 
145                 150                 155                 160 


Ile Arg Lys Ala Ile Ala Glu Lys Gly Tyr Arg Thr Val Gly Glu Leu 
                165                 170                 175     


Tyr Leu Lys Asp Pro Lys Tyr Asn Asp His Lys Arg Asn Lys Gly Tyr 
            180                 185                 190         


Ile Asp Gly Tyr Leu Ser Thr Pro Asn Arg Gln Met Leu Val Asp Glu 
        195                 200                 205             


Ile Lys Gln Ile Leu Asp Lys Gln Arg Glu Leu Gly Asn Glu Lys Leu 
    210                 215                 220                 


Thr Asp Glu Phe Tyr Ala Thr Tyr Leu Leu Gly Asp Glu Asn Arg Ala 
225                 230                 235                 240 


Gly Ile Phe Gln Ala Gln Arg Asp Phe Asp Glu Gly Pro Gly Ala Gly 
                245                 250                 255     


Pro Tyr Ala Gly Asp Gln Ile Lys Lys Met Val Gly Lys Asp Ile Phe 
            260                 265                 270         


Glu Pro Thr Glu Asp Arg Ala Ala Lys Ala Thr Tyr Thr Phe Gln Tyr 
        275                 280                 285             


Phe Asn Leu Leu Gln Lys Met Thr Ser Leu Asn Tyr Gln Asn Thr Thr 
    290                 295                 300                 


Gly Asp Thr Trp His Thr Leu Asn Gly Leu Asp Arg Gln Ala Ile Ile 
305                 310                 315                 320 


Asp Ala Val Phe Ala Lys Ala Glu Lys Pro Thr Lys Thr Tyr Lys Pro 
                325                 330                 335     


Thr Asp Phe Gly Glu Leu Arg Lys Leu Leu Lys Leu Pro Asp Asp Ala 
            340                 345                 350         


Arg Phe Asn Leu Val Asn Tyr Gly Ser Leu Gln Thr Gln Lys Glu Ile 
        355                 360                 365             


Glu Thr Val Glu Lys Lys Thr Arg Phe Val Asp Phe Lys Ala Tyr His 
    370                 375                 380                 


Asp Leu Val Lys Val Leu Pro Glu Glu Met Trp Gln Ser Arg Gln Leu 
385                 390                 395                 400 


Leu Asp His Ile Gly Thr Ala Leu Thr Leu Tyr Ser Ser Asp Lys Arg 
                405                 410                 415     


Arg Arg Arg Tyr Phe Ala Glu Glu Leu Asn Leu Pro Ala Glu Leu Ile 
            420                 425                 430         


Glu Lys Leu Leu Pro Leu Asn Phe Ser Lys Phe Gly His Leu Ser Ile 
        435                 440                 445             


Lys Ser Met Gln Asn Ile Ile Pro Tyr Leu Glu Met Gly Gln Val Tyr 
    450                 455                 460                 


Ser Glu Ala Thr Thr Asn Thr Gly Tyr Asp Phe Arg Lys Lys Gln Ile 
465                 470                 475                 480 


Ser Lys Asp Thr Ile Arg Glu Glu Ile Thr Asn Pro Val Val Arg Arg 
                485                 490                 495     


Ala Val Thr Lys Thr Ile Lys Ile Val Glu Gln Ile Ile Arg Arg Tyr 
            500                 505                 510         


Gly Lys Pro Asp Gly Ile Asn Ile Glu Leu Ala Arg Glu Leu Gly Arg 
        515                 520                 525             


Asn Phe Lys Glu Arg Gly Asp Ile Gln Lys Arg Gln Asp Lys Asn Arg 
    530                 535                 540                 


Gln Thr Asn Asp Lys Ile Ala Ala Glu Leu Thr Glu Leu Gly Ile Pro 
545                 550                 555                 560 


Val Asn Gly Gln Asn Ile Ile Arg Tyr Lys Leu His Lys Glu Gln Asn 
                565                 570                 575     


Gly Val Asp Pro Tyr Thr Gly Asp Gln Ile Pro Phe Glu Arg Ala Phe 
            580                 585                 590         


Ser Glu Gly Tyr Glu Val Asp His Ile Ile Pro Tyr Ser Ile Ser Trp 
        595                 600                 605             


Asp Asp Ser Tyr Thr Asn Lys Val Leu Thr Ser Ala Lys Cys Asn Arg 
    610                 615                 620                 


Glu Lys Gly Asn Arg Ile Pro Met Val Tyr Leu Ala Asn Asn Glu Gln 
625                 630                 635                 640 


Arg Leu Asn Ala Leu Thr Asn Ile Ala Asp Asn Ile Ile Arg Asn Ser 
                645                 650                 655     


Arg Lys Arg Gln Lys Leu Leu Lys Gln Lys Leu Ser Asp Glu Glu Leu 
            660                 665                 670         


Lys Asp Trp Lys Gln Arg Asn Ile Asn Asp Thr Arg Phe Ile Thr Arg 
        675                 680                 685             


Val Leu Tyr Asn Tyr Phe Arg Gln Ala Ile Glu Phe Asn Pro Glu Leu 
    690                 695                 700                 


Glu Lys Lys Gln Arg Val Leu Pro Leu Asn Gly Glu Val Thr Ser Lys 
705                 710                 715                 720 


Ile Arg Ser Arg Trp Gly Phe Leu Lys Val Arg Glu Asp Gly Asp Leu 
                725                 730                 735     


His His Ala Ile Asp Ala Thr Val Ile Ala Ala Ile Thr Pro Lys Phe 
            740                 745                 750         


Ile Gln Gln Val Thr Lys Tyr Ser Gln His Gln Glu Val Lys Asn Asn 
        755                 760                 765             


Gln Ala Leu Trp His Asp Ala Glu Ile Lys Asp Ala Glu Tyr Ala Ala 
    770                 775                 780                 


Glu Ala Gln Arg Met Asp Ala Asp Leu Phe Asn Lys Ile Phe Asn Gly 
785                 790                 795                 800 


Phe Pro Leu Pro Trp Pro Glu Phe Leu Asp Glu Leu Leu Ala Arg Ile 
                805                 810                 815     


Ser Asp Asn Pro Val Glu Met Met Lys Ser Arg Ser Trp Asn Thr Tyr 
            820                 825                 830         


Thr Pro Ile Glu Ile Ala Lys Leu Lys Pro Val Phe Val Val Arg Leu 
        835                 840                 845             


Ala Asn His Lys Ile Ser Gly Pro Ala His Leu Asp Thr Ile Arg Ser 
    850                 855                 860                 


Ala Lys Leu Phe Asp Glu Lys Gly Ile Val Leu Ser Arg Val Ser Ile 
865                 870                 875                 880 


Thr Lys Leu Lys Ile Asn Lys Lys Gly Gln Val Ala Thr Gly Asp Gly 
                885                 890                 895     


Ile Tyr Asp Pro Glu Asn Ser Asn Asn Gly Asp Lys Val Val Tyr Ser 
            900                 905                 910         


Ala Ile Arg Gln Ala Leu Glu Ala His Asn Gly Ser Gly Glu Leu Ala 
        915                 920                 925             


Phe Pro Asp Gly Tyr Leu Glu Tyr Val Asp His Gly Thr Lys Lys Leu 
    930                 935                 940                 


Val Arg Lys Val Arg Val Ala Lys Lys Val Ser Leu Pro Val Arg Leu 
945                 950                 955                 960 


Lys Asn Lys Ala Ala Ala Asp Asn Gly Ser Met Val Arg Ile Asp Val 
                965                 970                 975     


Phe Asn Thr Gly Lys Lys Phe Val Phe Val Pro Ile Tyr Ile Lys Asp 
            980                 985                 990         


Thr Val Glu Gln Val Leu Pro Asn  Lys Ala Ile Ala Arg  Gly Lys Ser 
        995                 1000                 1005             


Leu Trp  Tyr Gln Ile Thr Glu  Ser Asp Gln Phe Cys  Phe Ser Leu 
    1010                 1015                 1020             


Tyr Pro  Gly Asp Met Val His  Ile Glu Ser Lys Thr  Gly Ile Lys 
    1025                 1030                 1035             


Pro Lys  Tyr Ser Asn Lys Glu  Asn Asn Thr Ser Val  Val Pro Ile 
    1040                 1045                 1050             


Lys Asn  Phe Tyr Gly Tyr Phe  Asp Gly Ala Asp Ile  Ala Thr Ala 
    1055                 1060                 1065             


Ser Ile  Leu Val Arg Ala His  Asp Ser Ser Tyr Thr  Ala Arg Ser 
    1070                 1075                 1080             


Ile Gly  Ile Ala Gly Leu Leu  Lys Phe Glu Lys Tyr  Gln Val Asp 
    1085                 1090                 1095             


Tyr Phe  Gly Arg Tyr His Lys  Val His Glu Lys Lys  Arg Gln Leu 
    1100                 1105                 1110             


Phe Val  Lys Arg Asp Glu 
    1115                 


<210>  663
<211>  1119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  663

Met Asn Asp Lys Lys Asn Leu Gly Pro Leu Thr Phe Gly Phe Asp Ile 
1               5                   10                  15      


Gly Ile Ala Ser Val Gly Trp Ala Val Leu Ser Pro Thr Arg Ile Val 
            20                  25                  30          


Asp Leu Gly Val Arg Cys Phe Asp Lys Ala Glu Thr Ala Lys Glu Gly 
        35                  40                  45              


Glu Ser Leu Asn Leu Ala Arg Arg Ser Ala Arg Leu Met Arg Arg Arg 
    50                  55                  60                  


Leu Arg Arg Arg Ala Trp Arg Leu Ile Lys Leu Ala Arg Leu Leu Lys 
65                  70                  75                  80  


His Glu Gly Leu Ile Ala Asp Thr Lys Thr Phe Ile Gln Pro Ser Ala 
                85                  90                  95      


Ser His Ala Ser Pro Trp Gln Leu Arg Lys Asp Gly Leu Asp Arg Ala 
            100                 105                 110         


Leu Ser Pro Glu Glu Trp Ala Arg Val Leu Tyr His Leu Cys Lys His 
        115                 120                 125             


Arg Gly Phe His Trp Val Ser Arg Ala Glu Ala Arg Lys Ala Asp Gly 
    130                 135                 140                 


Asp Thr Lys Gly Glu Gly Gly Lys Val Lys Gln Gly Leu Ala Ser Thr 
145                 150                 155                 160 


Thr Lys Leu Met Asn Asp Lys Ser Tyr Arg Thr Ala Ala Glu Met Val 
                165                 170                 175     


Leu Ala Glu Phe Pro Ser Ala Gln Arg Asn Lys Arg Gly Glu Tyr Thr 
            180                 185                 190         


Lys Ala Leu Ser Arg Glu Leu Leu Asp Asn Glu Leu Lys Gln Leu Phe 
        195                 200                 205             


Glu Arg Gln Arg Glu Tyr Gly Asn Pro His Ala Asp Thr Asn Leu Glu 
    210                 215                 220                 


Ala Lys Ile Arg Gly Ser Gly Asp His Lys Ser Gly Leu Phe Trp Glu 
225                 230                 235                 240 


Gln Lys Pro Ala Leu Ser Gly Asn Asp Leu Leu Lys Met Leu Gly Lys 
                245                 250                 255     


Cys Thr Phe Glu Lys Gln Glu Tyr Arg Ala Pro Lys Ala Ser Phe Thr 
            260                 265                 270         


Ala Glu Arg His Val Trp Leu Thr Arg Leu Asn Asn Leu Arg Ile Ile 
        275                 280                 285             


Val Asp Gly Arg Thr Arg Pro Leu Ala Asp Ala Glu Arg Thr Met Ala 
    290                 295                 300                 


Leu Ser Leu Pro Tyr Glu Gln Val Gly Asp Phe Thr Tyr Lys Gln Leu 
305                 310                 315                 320 


Arg Ala Ala Leu Val Lys Ala Gly Leu Met Pro Asp Ser Phe Lys Phe 
                325                 330                 335     


Ala Ser Leu Ala Tyr Pro Ser Glu Phe Gln Lys Ala Glu Gly Lys Ala 
            340                 345                 350         


Lys Asp Pro Glu Ser Ala Ala Leu Thr Lys Leu Ala Ala Trp Gln Glu 
        355                 360                 365             


Leu Arg Ala Thr Leu Lys Lys Ala Asp Leu Glu Ser Glu Trp Gln Gln 
    370                 375                 380                 


Ile Ser Cys Ala Ala Leu Glu Gly Gln Pro Glu Thr Leu Asp Glu Ile 
385                 390                 395                 400 


Ala Gly Val Leu Ser Val Phe Lys Asp Asp Asp Glu Val Leu Asn Glu 
                405                 410                 415     


Leu Gln Lys Leu Gly Leu Pro Gly Gly Ala Lys Thr Glu Asn Ala Leu 
            420                 425                 430         


Leu Asp Ile Arg Phe Asp Lys Phe His Ala Leu Ser Leu Lys Ala Leu 
        435                 440                 445             


Arg Lys Ile Val Pro Tyr Met Glu Arg Gly Leu Arg Tyr Asp Glu Ala 
    450                 455                 460                 


Cys Glu Arg Ala Glu Tyr His His Ser Gln Leu His Lys Val Gly Glu 
465                 470                 475                 480 


Gly Asn Arg Lys Tyr Leu Pro Pro Leu Tyr Lys Ser Arg Asp Lys Asp 
                485                 490                 495     


Gly Arg Met Val Phe Asn Asp Glu Leu Gly Asp Ile Pro Arg Asn Pro 
            500                 505                 510         


Val Val Leu Arg Ser Leu Asn Gln Ala Arg Lys Val Leu Asn Ala Leu 
        515                 520                 525             


Ile Ala Arg Tyr Gly Ser Pro Ser Ser Val His Ile Glu Met Ala Arg 
    530                 535                 540                 


Glu Leu Ser Lys Pro Phe Asp Glu Arg Met Lys Val Gly Lys Glu Gln 
545                 550                 555                 560 


Glu Ala Tyr Arg Glu Arg Asn Glu Lys Asp Lys Gln Thr Phe Val Ala 
                565                 570                 575     


Glu Phe Gly Ile Val Gly Ala Ala Lys Gly Gly Glu Phe Glu Lys Trp 
            580                 585                 590         


Lys Leu Tyr Arg Glu Gln Gln Gly Lys Cys Ala Tyr Ser Ile Gln Gly 
        595                 600                 605             


Ile Asp Leu Asn Arg Leu Leu Glu Pro Gly Tyr Val Glu Ile Asp His 
    610                 615                 620                 


Ala Leu Pro Tyr Ser Arg Ser Phe Asp Asp Ser Lys Ser Asn Lys Val 
625                 630                 635                 640 


Leu Val Leu Ala Ala Glu Asn Arg Asn Lys Gly Asn Arg Thr Pro Tyr 
                645                 650                 655     


Glu Tyr Leu Asp Gly Glu His Asn Ser Glu Arg Trp Arg Gln Phe Val 
            660                 665                 670         


Gly Phe Val Glu Ser Asn Lys Leu Tyr Arg Gln Ala Lys Arg Ser Arg 
        675                 680                 685             


Leu Leu Arg Lys Asp Phe Gly Lys Glu Gln Ala Asp Glu Phe Arg Glu 
    690                 695                 700                 


Arg Asn Leu Asn Asp Thr Arg Tyr Val Cys Arg Phe Phe Lys Asn Gln 
705                 710                 715                 720 


Val Glu Gln Phe Leu Lys Leu Ala Glu Gly Ser Glu Ser Lys Arg Cys 
                725                 730                 735     


Val Val Leu Ser Gly Gln Leu Thr Ala Phe Leu Arg Ala Arg Trp Gly 
            740                 745                 750         


Leu Ile Lys Val Arg Asp Glu Ser Asp Arg His His Ala Leu Asp Ala 
        755                 760                 765             


Val Val Val Ala Ala Cys Thr His Gly Met Val Lys Arg Met Ser Asp 
    770                 775                 780                 


Tyr Ala Arg Arg Lys Glu Leu Asp Gln Val Ala Glu Gly Phe Val Asp 
785                 790                 795                 800 


Val Glu Thr Gly Glu Ile Ile Asn Ala Ala Met Phe Gln Gln Leu His 
                805                 810                 815     


Glu His Phe Pro Asp Pro Trp Pro His Phe Arg Asp Glu Val Gln Met 
            820                 825                 830         


Arg Leu Lys Ile Asp Asp Pro Ala Gln Leu Arg Ser Asp Leu Leu Arg 
        835                 840                 845             


Leu Gly Asn Tyr Ala Glu Glu Asp Val His Ala Val Arg Pro Leu Phe 
    850                 855                 860                 


Val Ser Arg Ala Pro Gln Arg Arg Asn Ser Gly Ala Ala His Lys Glu 
865                 870                 875                 880 


Thr Ile Tyr Gly Gln Pro Glu Arg Leu Lys Ala Gln Gly Gly Val Thr 
                885                 890                 895     


Glu Lys Val Ala Leu Thr Ala Leu Thr Met Lys Asp Leu Asp Arg Leu 
            900                 905                 910         


Ile Asp Pro His Arg Asn Glu Lys Leu Tyr Ala Ala Ile Arg Lys Arg 
        915                 920                 925             


Leu Glu Glu Phe Gly Gly Lys Ala Asp Lys Ala Phe Ala Ala Ser Asn 
    930                 935                 940                 


Pro Leu His Lys Pro Asp Lys Glu Gly Asn Pro Thr Gly Pro Ile Val 
945                 950                 955                 960 


Arg Thr Val Thr Leu Glu Ile Asp Lys Leu Ser Gly Val Pro Val Arg 
                965                 970                 975     


Gly Gly Ile Ala Lys Asn Asp Thr Met Leu Arg Val Asp Val Phe Thr 
            980                 985                 990         


Lys Ala Gly Lys Phe His Leu Val  Pro Val Tyr Val His  His Lys Val 
        995                 1000                 1005             


Thr Gly  Leu Pro Asn Arg Ala  Ile Val Ala Ser Lys  Asp Glu Asp 
    1010                 1015                 1020             


Glu Trp  Thr Ala Ile Asp Asp  Ser Phe Glu Phe Leu  Phe Ser Leu 
    1025                 1030                 1035             


Tyr Pro  Asn Asp Leu Val Thr  Ile Thr Gln Lys Gly  Lys Ala Pro 
    1040                 1045                 1050             


Val Arg  Gly Tyr Tyr Ala Ser  Cys His Arg Gly Thr  Gly Ala Phe 
    1055                 1060                 1065             


Ser Leu  Trp Ala His Asp Arg  Ala Ser Ser Val Gly  Lys Gly Gly 
    1070                 1075                 1080             


Gln Ile  Glu Gly Ile Gly Ile  Lys Thr Ala Leu Ala  Val Gln Lys 
    1085                 1090                 1095             


Tyr Asn  Val Asp Val Leu Gly  Thr Thr Tyr Ala Ala  Pro Leu Glu 
    1100                 1105                 1110             


Gln Arg  Arg Gly Leu Ala 
    1115                 


<210>  664
<211>  1120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  664

Met Arg Lys Val Thr Ile Gly Phe Asp Ile Gly Val Ser Ser Val Gly 
1               5                   10                  15      


Trp Ser Val Val Asp Met Asn Asn Gly Ala Ile Val Glu Thr Gly Val 
            20                  25                  30          


Arg Leu Phe Ser Ala Ser Ser Ala Glu Asn Asn Val Glu Arg Arg Asn 
        35                  40                  45              


Asn Arg Gln Thr Arg Arg Leu Asn Arg Arg Arg Lys Asn Arg Leu Lys 
    50                  55                  60                  


Asp Val Lys Tyr Leu Leu Glu Thr Asn His Phe Thr Phe Asp Gln Glu 
65                  70                  75                  80  


Gln Gly Thr Asn Pro Tyr Glu Leu Arg Val Ala Gly Leu His Arg Lys 
                85                  90                  95      


Leu Thr Arg Ser Glu Leu Ala Arg Ala Leu Tyr His Leu Val Lys Arg 
            100                 105                 110         


Arg Gly Ile Ser Tyr Asp Leu Thr Asp Ile Glu Glu Glu Glu Ile Val 
        115                 120                 125             


Thr Thr Ser Ala Tyr Lys Thr Ala Leu Gly Lys Asn Gln Ser Gln Leu 
    130                 135                 140                 


Ala Glu Lys Thr Pro Gly Glu Ile Gln Phe Ala Arg Leu Glu Asn Tyr 
145                 150                 155                 160 


Gly Lys Val Arg Gly Ser Ile Glu Ile Asn Glu Gln Asp Ile Leu Leu 
                165                 170                 175     


Asn Ile Phe Pro Thr Ser Ala Tyr Leu Asn Glu Ala Lys Arg Ile Leu 
            180                 185                 190         


Ala Thr Gln Arg Lys Phe Tyr Pro Glu Ile Thr Glu Ala Phe Ile Glu 
        195                 200                 205             


Lys Tyr Cys Thr Ile Leu Thr Arg Lys Arg Glu Tyr Tyr Val Gly Pro 
    210                 215                 220                 


Gly Thr Glu Lys Asn Arg Thr Asp Tyr Gly Ile Tyr Arg Thr Asn Gly 
225                 230                 235                 240 


Glu Thr Leu Asp Asn Leu Phe Glu Ile Leu Ile Gly Lys Asp Lys Phe 
                245                 250                 255     


Tyr Pro Asn Glu Glu Arg Ala Ala Gly Asn Ser Tyr Thr Ala Gln Leu 
            260                 265                 270         


Phe Asn Phe Val Asn Asp Leu Asn Asn Leu Lys Ile Gln Thr Ile Glu 
        275                 280                 285             


Asp Gln Lys Leu Thr Pro Lys Gln Lys Glu Glu Ile Ile Glu Gln Val 
    290                 295                 300                 


Lys Thr Ala Glu Lys Lys Val Ser Leu Ile Gln Leu Ile Ser Lys Ile 
305                 310                 315                 320 


Thr Lys Thr Pro Lys Asp Gln Ile Thr Gly Tyr Arg Thr Asp Lys Asn 
                325                 330                 335     


Gly Lys Pro Asp Leu His Ser Met Ala Val Phe Arg Lys Val Arg Lys 
            340                 345                 350         


Gln Phe Leu Thr Met Glu Ile Asp Ile Leu His Trp Pro Asp Glu Leu 
        355                 360                 365             


Leu Asn Asp Leu Gly Arg Leu Leu Thr Leu Asn Thr Glu Lys Gly Glu 
    370                 375                 380                 


Ile Arg Lys Gln Leu Thr Lys Asp Phe Met Lys Arg Tyr Pro Ile Leu 
385                 390                 395                 400 


Asn Glu Gln Leu Val Asp Gln Ile Ile Glu Asn Arg His Ile Phe Ala 
                405                 410                 415     


Val Ser Gly Asn His Lys Trp His Arg Phe Ser Leu Arg Thr Met Asn 
            420                 425                 430         


Glu Leu Leu Pro Glu Met Leu Ala Thr Ser Lys Glu Gln Met Thr Leu 
        435                 440                 445             


Leu Ser Glu Arg Gly Met Ile Lys Gln His Lys Arg Asp Tyr Leu Lys 
    450                 455                 460                 


Met Asn Glu Leu Asp Ala Arg Leu Ile Ser Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Gly Lys Ser Val Arg Glu Ala Ile Lys Leu Phe Asn Glu Leu 
                485                 490                 495     


Met Lys Lys Tyr Gln Glu Val Arg Tyr Val Val Leu Glu Met Pro Arg 
            500                 505                 510         


Asp Asp Asn Glu Glu Lys Ala Lys Lys Glu Leu Gln Lys Tyr Gln Lys 
        515                 520                 525             


Glu Asn Glu Asn Glu Lys Glu Ile Ala Leu Ser Glu Phe Gln Arg Lys 
    530                 535                 540                 


Gly Phe Phe Ser Asp Ala Gln Leu Glu Arg Ala Leu Ile Lys Asp Arg 
545                 550                 555                 560 


Lys Leu Lys Thr Lys Ile Arg Phe Trp His Gln Gln Glu Gly Lys Cys 
                565                 570                 575     


Leu Tyr Ser Gly Gln Ile Ile Thr Pro Val Glu Leu Leu Thr Asn Ser 
            580                 585                 590         


Phe Ser Phe Asp Ile Asp His Ile Ile Pro Gln Ser Val Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Asn Asn Lys Val Leu Cys Thr Lys Glu Met Asn Asp Gln 
    610                 615                 620                 


Lys Gly Lys Gln Thr Pro Tyr Gly Phe Leu Leu Asn Gly Asn Gly Gln 
625                 630                 635                 640 


Gly Phe Asp Arg Phe Lys Ala Met Val Asn Ala Asn Arg Leu Met Ser 
                645                 650                 655     


Lys Gly Lys Lys Lys Asn Leu Leu Phe Met Glu Asp Leu Asn Asn Ile 
            660                 665                 670         


Glu Thr Arg Lys Arg Phe Ile Ala Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ala Ser Arg Val Val Leu Asn Glu Ile Gln Gln Tyr Leu Arg Ala Lys 
    690                 695                 700                 


Asn Lys Asp Thr Gln Val Ala Val Val Arg Gly Lys Phe Thr Ala Thr 
705                 710                 715                 720 


Leu Arg Lys His Trp Asn Ile Asn Lys Ser Arg Glu Thr His His His 
                725                 730                 735     


His Ala Leu Asp Ala Ser Ile Ile Ala Ile Thr Pro Leu Leu Ser Val 
            740                 745                 750         


Trp Lys Lys Lys Gln Thr Leu Ile Pro Lys Thr Leu Ser Glu Glu Lys 
        755                 760                 765             


Leu Glu Phe Glu Tyr Asp Asp Ile Val Glu Asp Thr Ile Phe Glu Lys 
    770                 775                 780                 


Ala Leu Tyr Gln Leu Pro Lys Glu Asn Phe Lys Glu Gln Leu Asn Asn 
785                 790                 795                 800 


Ser Gln Pro Asn Ile Lys Phe Ser Tyr Gln Val Asp Lys Lys Met Asn 
                805                 810                 815     


Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser Val Lys Gln Ala Lys Leu 
            820                 825                 830         


Ser Ser Asp Lys Gln Ser Glu Glu Tyr Val Leu Ala Lys Ile Lys Asp 
        835                 840                 845             


Ile Tyr Ser Val Glu Gly Tyr Lys Thr Phe Ala Lys Ile Tyr Glu Lys 
    850                 855                 860                 


Asp Lys Ser Lys Phe Leu Met Ala Gln Asn Asp Pro Lys Thr Phe Glu 
865                 870                 875                 880 


Lys Leu Glu Glu Ile Ile Gln Ser Tyr Pro Ser Thr Arg Glu Ile Ile 
                885                 890                 895     


Val Gly Glu Lys Val Lys Val Val Asp Val Ser Pro Phe Glu Leu Tyr 
            900                 905                 910         


Arg Lys Glu Tyr Gly Tyr Ile Thr Lys Tyr Ala Lys Lys Asn Asn Gly 
        915                 920                 925             


Pro Ile Ile Arg Gln Leu Lys Tyr Tyr Asp His Lys Ile Gly Met His 
    930                 935                 940                 


Leu Asp Ile Thr Asn Gln Lys Asn Asn Gly Lys Gly Lys Lys Val Ile 
945                 950                 955                 960 


Leu Gln Gln Leu Lys Ser Trp Arg Thr Asp Val Tyr Tyr Asn His Glu 
                965                 970                 975     


Lys Gln Glu Tyr Glu Ile Met Gly Leu Lys Tyr Ser Asp Leu Arg Phe 
            980                 985                 990         


Lys Lys Asp Gln Tyr Gly Ile Pro  Lys Glu Arg Tyr Gln  Glu Val Lys 
        995                 1000                 1005             


Gln Met  Glu Lys Ile Ala Pro  Gln Ser Glu Phe Cys  Phe Ser Leu 
    1010                 1015                 1020             


Tyr Arg  Asn Asp Arg Ile Lys  Val Leu Asn Lys Glu  Thr Asp Glu 
    1025                 1030                 1035             


Glu Val  Glu Leu Leu Phe Gly  Ala Arg Asn Tyr Thr  Ser Ala Gly 
    1040                 1045                 1050             


Tyr Val  Asp Leu Lys Pro Ile  Asp Arg Val Glu Phe  Gly Lys Lys 
    1055                 1060                 1065             


Glu Trp  Ile Pro Val Tyr Gln  Tyr Ala Thr Ser Asp  Gly Lys Cys 
    1070                 1075                 1080             


Lys Lys  Lys Phe Ala Lys Lys  Gly Tyr Glu Ile Tyr  Lys Val Asn 
    1085                 1090                 1095             


Thr Thr  Ile Leu Gly Glu Ser  Tyr Tyr Ile Lys Lys  Glu Arg Met 
    1100                 1105                 1110             


Leu Pro  Lys Asn Ile Leu Asp  
    1115                 1120 


<210>  665
<211>  1120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  665

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn Arg Leu Phe Glu Glu Ile Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Arg Thr Ser Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Phe Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
    290                 295                 300                 


Ile Asn Tyr Val Lys Asn Glu Lys Ala Met Gly Pro Ala Lys Leu Phe 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
385                 390                 395                 400 


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
                405                 410                 415     


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
            420                 425                 430         


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
                485                 490                 495     


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
        515                 520                 525             


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Pro 
            580                 585                 590         


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Phe Asp Val Arg Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
    690                 695                 700                 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Asn 
            740                 745                 750         


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Asp Gln 
        755                 760                 765             


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
    770                 775                 780                 


Glu Ser Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
785                 790                 795                 800 


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
                805                 810                 815     


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
            820                 825                 830         


Ala Lys Val Gly Lys Asp Lys Ala Asp Glu Thr Tyr Val Leu Gly Lys 
        835                 840                 845             


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
865                 870                 875                 880 


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
                885                 890                 895     


Gln Met Asn Glu Lys Gly Lys Glu Val Pro Cys Asn Pro Phe Leu Lys 
            900                 905                 910         


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
        915                 920                 925             


Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Lys Asp Ser Asn Asn Lys Val Val Leu Gln 
945                 950                 955                 960 


Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn Lys Asn Thr Gly 
                965                 970                 975     


Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ser Asp Met Gln Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Lys Tyr Ser Ile Ser  Lys Glu Gln Tyr Glu  Asn Ile Lys 
        995                 1000                 1005             


Val Arg  Glu Gly Val Asp Glu  Asn Ser Glu Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Asn Asp Leu Leu Leu  Leu Lys Asp Ser Glu  Asn Gly Glu 
    1025                 1030                 1035             


Gln Ile  Leu Leu Arg Phe Thr  Ser Arg Asn Asp Thr  Ser Lys His 
    1040                 1045                 1050             


Tyr Val  Glu Leu Lys Pro Tyr  Asn Arg Gln Lys Phe  Glu Gly Ser 
    1055                 1060                 1065             


Glu Tyr  Leu Ile Lys Ser Leu  Gly Thr Val Val Lys  Gly Gly Arg 
    1070                 1075                 1080             


Cys Ile  Lys Gly Leu Gly Lys  Ser Asn Ile Ser Ile  Tyr Lys Val 
    1085                 1090                 1095             


Arg Thr  Asp Val Leu Gly Asn  Gln His Ile Ile Lys  Asn Glu Gly 
    1100                 1105                 1110             


Asp Lys  Pro Lys Leu Asp Phe  
    1115                 1120 


<210>  666
<211>  1120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  666

Met Gly Leu Asp Ile Gly Val Ala Ser Val Gly Phe Ser Val Leu Asp 
1               5                   10                  15      


Ile Asp Lys Gly Lys Val Ile Glu Leu Gly Ala Arg Leu Phe Ser Ala 
            20                  25                  30          


Thr Val Ala Ala Gly Asn Gln Asp Arg Arg Asp Met Arg Gly Ala Arg 
        35                  40                  45              


Arg Leu Leu Asn Arg Asn Lys Gln Arg Arg Gln Asp Thr Gly Lys Leu 
    50                  55                  60                  


Phe Lys Lys Phe Gly Leu Ile Asp Asp Phe Asp Lys Gly Ser Phe Tyr 
65                  70                  75                  80  


Asp Asn Phe Asn Gln Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly 
                85                  90                  95      


Leu Thr Glu Gln Leu Thr Lys Glu Glu Leu Ala Glu Ser Leu Tyr Gln 
            100                 105                 110         


Ile Val Lys His Arg Gly Ile Ser Tyr Ala Leu Lys Asp Ala Asp Val 
        115                 120                 125             


Asp Glu Gly Gly Thr Asp Tyr Ser Val Ser Leu Lys Ile Asn Ser Gln 
    130                 135                 140                 


Glu Leu Ala Glu Lys Thr Pro Ala Gln Ile Gln Leu Gln Arg Leu Asn 
145                 150                 155                 160 


Asp Tyr Gly Lys Val Arg Gly Gln Val Val Ile Gly Asp Asp Pro Asp 
                165                 170                 175     


Asn Gln Lys Val Leu Leu Asn Val Phe Pro Thr Ser Ala Tyr Glu Lys 
            180                 185                 190         


Glu Ala Lys Gln Ile Ile Ala Thr Gln Gln Gln Phe Tyr Pro Glu Ser 
        195                 200                 205             


Leu Thr Asp Lys Phe Thr Glu Glu Tyr Cys Gln Ile Leu Thr Arg Lys 
    210                 215                 220                 


Arg Asp Tyr Phe Val Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr 
225                 230                 235                 240 


Gly Ile Tyr Lys Thr Asp Gly Arg Thr Leu Asp Asn Leu Phe Glu Glu 
                245                 250                 255     


Leu Ile Gly His Asp Lys Ile Tyr Pro Glu Glu Leu Arg Ala Ser Ala 
            260                 265                 270         


Ala Ser Tyr Thr Ala Gln Leu Phe Asn Val Leu Asn Asp Leu Asn Asn 
        275                 280                 285             


Leu Arg Ile Leu Asn Tyr Glu Asp Gly Lys Leu Thr Lys Glu Asp Lys 
    290                 295                 300                 


Glu Lys Ile Ile Ala Glu Ile Lys Asn Asn Thr Thr Thr Ile Asn Met 
305                 310                 315                 320 


Leu Asn Val Ile Lys Lys Val Ala Gly Cys Ser Lys Asp Asp Ile Lys 
                325                 330                 335     


Gly Phe Arg Val Asn Glu Lys Asp Lys Pro Glu Ile Ser Ser Met Pro 
            340                 345                 350         


Val Tyr Arg Lys Ile His Lys Asp Leu Leu Lys Ala Gly Val Asp Ile 
        355                 360                 365             


Ser Asp Trp Pro Val Glu Phe Ile Asp Glu Leu Ser Phe Ile Leu Thr 
    370                 375                 380                 


Leu Asn Thr Glu Asn Gly Glu Ile Arg Lys Gln Leu Asn Asn Arg Leu 
385                 390                 395                 400 


Ala Pro Lys Phe Asp Phe Leu Asn Ala Asp Leu Ile Gln Leu Ile Ile 
                405                 410                 415     


Asp Asn Lys Asp Ser Phe Glu Ile Lys Thr Asn Asn Lys Trp His Arg 
            420                 425                 430         


Phe Ser Val Lys Thr Met Asn Lys Leu Ile Pro Glu Met Met Glu Arg 
        435                 440                 445             


Pro Val Glu Gln Met Thr Leu Leu Asn Glu Met Gly Leu Val Lys Lys 
    450                 455                 460                 


Asp Lys Lys Arg Phe Glu Asn Asn Lys Tyr Leu Pro Tyr Lys Glu Ile 
465                 470                 475                 480 


Ala Lys Asp Ile Phe Asn Pro Val Ala Ser Lys Ser Val Arg Glu Ala 
                485                 490                 495     


Leu Lys Ile Val Asn Ala Val Leu Lys Lys Tyr Gly His Ile Asp Tyr 
            500                 505                 510         


Leu Val Val Glu Met Pro Arg Asp Lys Asn Leu Lys Glu Glu Gln Asp 
        515                 520                 525             


Asn Ile Lys Glu Phe Gln Asn Lys Asn Lys Lys Ala Lys Asp Ala Ala 
    530                 535                 540                 


Phe Glu Ala Phe Val Lys Ser Val Gly Ser Glu Gln Arg Val Lys Glu 
545                 550                 555                 560 


Ala Leu Ser Lys Asn Arg Lys Leu Gln Met Lys Met Arg Leu Trp Tyr 
                565                 570                 575     


Gln Gln Gln Glu Ile Asp Pro Tyr Asn Gly Lys Thr Ile Asp Ala Thr 
            580                 585                 590         


Asp Leu Ile Asn Asn Pro Asp Lys Phe Glu Ile Asp His Ile Ile Pro 
        595                 600                 605             


Gln Ser Ile Ser Phe Tyr Asp Ser Ile Asn Asn Lys Thr Leu Cys Phe 
    610                 615                 620                 


Ala Ser Met Asn Gln Val Lys Gly Gln Lys Thr Pro Tyr Glu Phe Met 
625                 630                 635                 640 


Leu Glu Gly His Gly Gln Ser Tyr Asp Lys Phe Lys Ala Thr Val Met 
                645                 650                 655     


Ala Asn Lys Asn Phe Gly Lys Ala Lys Arg Ala Asn Tyr Leu Phe Glu 
            660                 665                 670         


Glu Asn Val Ser Asp Ile Glu Thr Arg Lys Arg Phe Leu Ser Arg Asn 
        675                 680                 685             


Leu Val Asp Thr Arg Tyr Ser Ser Arg Val Val Leu Asn Ser Leu Gln 
    690                 695                 700                 


Asp Phe Phe Arg Glu Lys Ser Ala Asp Thr Lys Val Thr Val Ile Arg 
705                 710                 715                 720 


Gly Lys Phe Thr Ser Asn Met Arg Lys His Trp His Ile Asp Lys Thr 
                725                 730                 735     


Arg Glu Thr Phe His His His Ala Ile Asp Ala Ser Ile Ile Ala Ala 
            740                 745                 750         


Thr Pro Phe Leu Arg Met Trp Lys Lys Gly Gly Thr Ile Phe Pro Val 
        755                 760                 765             


Lys Val Gly Glu Glu Ser Ile Asp Ile Glu Thr Gly Glu Ile Leu Asp 
    770                 775                 780                 


Asp Lys Asn Phe Asp Lys Ala Met Tyr Glu Glu Pro Tyr Ser Gly Phe 
785                 790                 795                 800 


Val Ser Glu Ile Met Asn Ala Asp Asp Arg Ile Lys Phe Ser His Gln 
                805                 810                 815     


Val Asp Lys Lys Met Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Thr Gly Lys Leu Ala Lys Asp Lys Lys Asp Ala Glu Tyr Ile 
        835                 840                 845             


Val Ala Lys Val Lys Asp Ile Tyr Ser Val Asp Gly Phe Lys Lys Phe 
    850                 855                 860                 


Lys Lys Val Tyr Asp Lys Asp Lys Thr Lys Phe Leu Leu Tyr Lys Tyr 
865                 870                 875                 880 


Asp Pro Arg Thr Phe Ser Lys Leu Glu Arg Ile Ile Ser Asp Cys Pro 
                885                 890                 895     


Asp Lys Val Glu Lys Val Gln Thr Asn Gly Lys Val Lys Ala Val Asp 
            900                 905                 910         


Ile Ser Pro Phe Glu Met Tyr Arg Arg Asp His Gly Met Ile Lys Lys 
        915                 920                 925             


Tyr Ser Lys Lys Gly Asn Gly Pro Ala Ile Lys Gln Leu Lys Tyr Leu 
    930                 935                 940                 


Asp Lys Lys Leu Gly Ser His Ile Asp Ile Thr Pro Ala Asn Ala Asn 
945                 950                 955                 960 


Gly Lys His Val Ile Leu Gln Ser Leu Lys Pro Trp Arg Thr Asp Val 
                965                 970                 975     


Tyr Leu Asn His Glu Thr Gly Glu Tyr Glu Ile Met Gly Ile Lys Tyr 
            980                 985                 990         


Ser Asp Leu Lys Phe Asn Lys Asn  Glu Gly Tyr Gly Ile  Lys Lys Asp 
        995                 1000                 1005             


Lys Tyr  Leu Glu Ile Lys Lys  Val Glu Glu Val Ser  Glu Lys Ser 
    1010                 1015                 1020             


Glu Phe  Met Phe Ser Leu Tyr  Arg Lys Asp Arg Val  Lys Val Gln 
    1025                 1030                 1035             


Asp Met  Lys Thr Gly Glu Ser  Val Glu Leu Leu Phe  Trp Ser Arg 
    1040                 1045                 1050             


Asn Phe  Ser Asn Lys Lys Tyr  Ala Glu Leu Lys Pro  Ile Ser Gln 
    1055                 1060                 1065             


Ala Glu  Asn Asp Lys Lys Leu  Pro Val Tyr Gly Lys  Gly Arg Leu 
    1070                 1075                 1080             


Ile Lys  Arg Leu Ile Pro Lys  Asn Cys Lys Ile Trp  Lys Val Asn 
    1085                 1090                 1095             


Thr Thr  Ile Leu Gly Asp Pro  Tyr Tyr Leu Glu Lys  Glu Ser Asp 
    1100                 1105                 1110             


Ser Pro  Lys Asp Ile Leu Asp  
    1115                 1120 


<210>  667
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  667

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn Arg Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Ile Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Val Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Arg Thr Asn Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Phe Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
    290                 295                 300                 


Ile Asn Tyr Val Lys Asn Glu Lys Ala Met Gly Pro Ala Lys Leu Phe 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
385                 390                 395                 400 


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
                405                 410                 415     


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
            420                 425                 430         


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
                485                 490                 495     


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
        515                 520                 525             


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Ser 
            580                 585                 590         


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Phe Asp Val Arg Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
    690                 695                 700                 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Asn 
            740                 745                 750         


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Asp Gln 
        755                 760                 765             


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
    770                 775                 780                 


Glu Phe Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
785                 790                 795                 800 


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
                805                 810                 815     


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
            820                 825                 830         


Ala Lys Val Gly Lys Asp Lys Ala Asp Glu Thr Tyr Val Leu Gly Lys 
        835                 840                 845             


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
865                 870                 875                 880 


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
                885                 890                 895     


Gln Met Asn Glu Lys Gly Lys Glu Val Pro Cys Asn Pro Phe Leu Lys 
            900                 905                 910         


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
        915                 920                 925             


Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Lys Asp Ser Asn Asn Lys Val Val Leu Gln 
945                 950                 955                 960 


Ser Val Ser Pro Trp Arg Ala Asp Val Tyr Phe Asn Asn Thr Thr Gly 
                965                 970                 975     


Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Thr Tyr Lys Ile Ser  Gln Glu Lys Tyr Asn  Asp Ile Lys 
        995                 1000                 1005             


Lys Lys  Glu Gly Val Asp Ser  Asp Ser Glu Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Asn Asp Leu Leu Leu  Val Lys Glu Thr Glu  Thr Lys Glu 
    1025                 1030                 1035             


Gln Gln  Leu Phe Arg Phe Leu  Ser Arg Thr Met Pro  Lys Gln Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Asp Lys Gln Lys  Phe Glu Gly 
    1055                 1060                 1065             


Gly Glu  Ala Leu Ile Lys Val  Leu Gly Asn Val Ala  Asn Gly Gly 
    1070                 1075                 1080             


Gln Cys  Ile Lys Gly Leu Gly  Lys Ser Asn Ile Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Val Arg  Thr Asp Val Leu Gly  Asn Gln His Ile Ile  Lys Asn Glu 
    1100                 1105                 1110             


Gly Asp  Lys Pro Lys Leu Asp  Phe 
    1115                 1120     


<210>  668
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  668

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asn Ser Gly Lys Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Lys 
        35                  40                  45              


Asn Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Lys His Arg Gly Val 
    50                  55                  60                  


Arg Phe Gln Asp Leu Phe Glu Asp Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asn Lys Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Thr Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Cys Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Ser Lys Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Lys Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asn Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Ser 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Thr Ile 
    290                 295                 300                 


Ile Glu Tyr Ala Lys Ser Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Thr Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Gly Glu Leu Ser 
        355                 360                 365             


Arg Asn Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr Lys Leu Lys Asp Ser Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys Glu Thr Ser Lys Arg Ser 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asp Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Asn Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Thr Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Val Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Gly Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Glu Ser Lys Leu Leu Gly 
                645                 650                 655     


Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Asn Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Ala His 
    690                 695                 700                 


Lys Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys His Asn Asn Pro Leu Ile Ser Tyr Lys Glu Gly Arg Phe 
        755                 760                 765             


Ile Asp Ser Glu Thr Gly Glu Ile Ile Ser Leu Ser Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Arg Ser Lys Thr Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Asp Lys Ser Glu Glu Thr Tyr Val Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Gln Ala Gly Tyr Asp Ala Phe Ile Lys 
    850                 855                 860                 


Ile Tyr Asn Lys Asp Lys Ser Lys Phe Leu Met Tyr His Lys Asp Pro 
865                 870                 875                 880 


Gln Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Arg Thr Tyr Pro Ser 
                885                 890                 895     


Lys Glu Leu Asn Asp Lys Asn Lys Glu Ile Pro Cys Asn Pro Phe Glu 
            900                 905                 910         


Lys Tyr Arg Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly 
        915                 920                 925             


Asn Gly Pro Glu Ile Lys Ser Met Lys Tyr Tyr Asp Lys Asn Ile Pro 
    930                 935                 940                 


Glu Ser Phe Ile Asn Ile Thr Pro Ser Asn Ser Asn Asn Lys Val Leu 
945                 950                 955                 960 


Leu Arg Gln Leu Tyr Pro Trp Arg Thr Asp Val Tyr Phe Asn Pro Gln 
                965                 970                 975     


Thr Asp Lys Tyr Glu Leu Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe 
            980                 985                 990         


Glu Lys Ala Thr Gly Lys Tyr Ser  Ile Thr Ile Asp Lys  Tyr Asn Glu 
        995                 1000                 1005             


Ile Lys  Glu Lys Glu Gly Val  Ser Lys Glu Ser Glu  Phe Lys Phe 
    1010                 1015                 1020             


Thr Leu  Tyr Lys Asn Asp Leu  Leu Leu Ile Lys Asp  Thr Lys Asn 
    1025                 1030                 1035             


Asn Glu  Glu Gln Ile Phe Arg  Phe Leu Ser Arg Thr  Met Pro Asn 
    1040                 1045                 1050             


Gln Asn  His Tyr Val Glu Leu  Lys Pro Tyr Asp Lys  Gly Asn Phe 
    1055                 1060                 1065             


Glu Gly  Lys Glu Lys Ile Lys  Val Phe Lys Met Thr  Ser Asn Gly 
    1070                 1075                 1080             


Arg Cys  Ile Lys Gly Leu Gly  Lys Lys Tyr Leu Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Val Lys  Thr Asp Ile Leu Gly  Asn Lys His Phe Ile  Lys Lys Glu 
    1100                 1105                 1110             


Gly Asp  Glu Pro Lys Leu Lys  Phe 
    1115                 1120     


<210>  669
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  669

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asn Ser Gly Lys Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Lys 
        35                  40                  45              


Asn Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Lys His Arg Gly Val 
    50                  55                  60                  


Arg Phe Gln Asp Leu Phe Glu Asp Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asn Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Ala Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Arg Lys Glu 
            180                 185                 190         


Ala Glu Lys Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Val Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asn Glu Tyr Arg Ala Ser Lys Ser Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Val Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Glu Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Val Lys Met Ile Asp Ala Ser Val Asp Gln Ile Ser Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Gly Glu Leu Ser 
        355                 360                 365             


Arg Asn Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Ser Lys Leu Met Gly Val Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys His Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Gly Asn Ile Phe Asn Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Thr Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val His Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Asp Leu Lys Ser Leu Ser 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Ser His 
    690                 695                 700                 


Gln Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Pro Leu Ile Thr Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Ile Asp Ser Gln Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


His Ser Lys Thr Phe Glu Asp Gly Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Asp Lys Ser Glu Glu Thr Tyr Val Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Gln Ala Gly Tyr Asp Ala Phe Ile Lys 
    850                 855                 860                 


Ile Tyr Asn Lys Asp Lys Arg Lys Phe Leu Met Tyr His Lys Asp Pro 
865                 870                 875                 880 


Gln Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Arg Thr Tyr Pro Ser 
                885                 890                 895     


Lys Glu Arg Asn Asp Lys Asn Lys Glu Val Pro Cys Asn Pro Phe Glu 
            900                 905                 910         


Lys Tyr Arg Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly 
        915                 920                 925             


Asn Gly Pro Glu Ile Lys Cys Leu Lys Tyr Tyr Asp Asn Lys Leu Gly 
    930                 935                 940                 


Asn Tyr Ile Asp Ile Ser Pro Asp Gly Ser Asp Asn Gln Val Val Leu 
945                 950                 955                 960 


Gln Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn His Gln Thr 
                965                 970                 975     


Lys Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu Ser Phe Glu 
            980                 985                 990         


Lys Gly Ser Gly Lys Tyr Ser Ile  Ser Asn Glu Lys Tyr  Asn Ser Ile 
        995                 1000                 1005             


Lys Arg  Ile Glu Gly Val Asn  Glu Gln Ser Glu Phe  Lys Phe Thr 
    1010                 1015                 1020             


Leu Tyr  Lys Asn Asp Leu Ile  Leu Ile Lys Asp Ser  Glu Asn Asn 
    1025                 1030                 1035             


Glu Gln  Lys Leu Phe Arg Phe  Asn Ser Arg Asn Asp  Thr Ala Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Asp Lys Ala Arg  Phe Asp Gly 
    1055                 1060                 1065             


Gln Gln  Glu Leu Ile Thr Ile  Leu Gly Asn Val Ala  Lys Gly Gly 
    1070                 1075                 1080             


Gln Cys  Leu Lys Gly Leu Asn  Lys Ser Asn Ile Ser  Ile Phe Lys 
    1085                 1090                 1095             


Val Lys  Thr Asp Val Leu Gly  Lys Lys His Ile Ile  Lys Lys Glu 
    1100                 1105                 1110             


Gly Asp  Glu Pro Lys Leu Lys  Phe 
    1115                 1120     


<210>  670
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  670

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Ile Val 
    50                  55                  60                  


Arg Leu Asn Arg Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Lys Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Arg Thr Asn Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Glu Glu Phe Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
    290                 295                 300                 


Ile Asn Tyr Val Lys Asn Glu Lys Ala Met Gly Pro Ala Lys Leu Phe 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
385                 390                 395                 400 


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
                405                 410                 415     


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
            420                 425                 430         


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
                485                 490                 495     


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
        515                 520                 525             


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Pro 
            580                 585                 590         


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Phe Asp Val Gln Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
    690                 695                 700                 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Asn 
            740                 745                 750         


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Asp Gln 
        755                 760                 765             


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
    770                 775                 780                 


Glu Ser Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
785                 790                 795                 800 


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
                805                 810                 815     


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
            820                 825                 830         


Ala Lys Val Gly Lys Asp Lys Lys Asp Glu Thr Tyr Val Leu Gly Lys 
        835                 840                 845             


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
865                 870                 875                 880 


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
                885                 890                 895     


Glu Met Asn Glu Lys Gly Lys Glu Val Pro Cys Asn Pro Phe Leu Lys 
            900                 905                 910         


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
        915                 920                 925             


Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Lys Asn Ser Asn Asn Lys Val Val Leu Gln 
945                 950                 955                 960 


Ser Val Ser Pro Trp Arg Ala Asp Val Tyr Phe Asn Lys Thr Thr Gly 
                965                 970                 975     


Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Thr Tyr Lys Ile Ser  Gln Glu Lys Tyr Asn  Asp Ile Lys 
        995                 1000                 1005             


Lys Lys  Glu Gly Val Asp Ser  Asp Ser Glu Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Asn Asp Leu Leu Leu  Val Lys Asp Thr Glu  Thr Lys Glu 
    1025                 1030                 1035             


Gln Gln  Leu Phe Arg Phe Leu  Ser Arg Thr Met Pro  Lys Lys Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Asp Lys Gln Lys  Phe Glu Gly 
    1055                 1060                 1065             


Gly Glu  Ala Leu Ile Lys Val  Leu Gly Asn Val Ala  Asn Ser Gly 
    1070                 1075                 1080             


Gln Cys  Ile Lys Gly Leu Ala  Lys Ser Asn Ile Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Val Arg  Thr Asp Val Leu Gly  Asn Gln His Ile Ile  Lys Asn Glu 
    1100                 1105                 1110             


Gly Asp  Lys Pro Lys Leu Asp  Phe 
    1115                 1120     


<210>  671
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  671

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn Arg Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Ile Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Arg Thr Ser Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Phe Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
    290                 295                 300                 


Ile Asn Tyr Val Lys Asn Glu Lys Ala Met Gly Pro Ala Lys Leu Phe 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
385                 390                 395                 400 


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
                405                 410                 415     


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
            420                 425                 430         


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
                485                 490                 495     


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
        515                 520                 525             


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Ser 
            580                 585                 590         


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Phe Asp Val Arg Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
    690                 695                 700                 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Asn 
            740                 745                 750         


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Asp Gln 
        755                 760                 765             


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
    770                 775                 780                 


Glu Ser Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
785                 790                 795                 800 


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
                805                 810                 815     


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
            820                 825                 830         


Ala Lys Val Gly Lys Asp Lys Ala Asp Glu Thr Tyr Val Leu Gly Lys 
        835                 840                 845             


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
865                 870                 875                 880 


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
                885                 890                 895     


Gln Ile Asn Glu Lys Gly Lys Glu Val Pro Cys Asn Pro Phe Leu Lys 
            900                 905                 910         


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
        915                 920                 925             


Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Lys Asp Ser Asn Asn Lys Val Val Leu Gln 
945                 950                 955                 960 


Ser Val Ser Pro Trp Arg Ala Asp Val Tyr Phe Asn Lys Thr Thr Gly 
                965                 970                 975     


Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Thr Tyr Lys Ile Ser  Gln Glu Lys Tyr Asn  Asp Ile Lys 
        995                 1000                 1005             


Lys Lys  Glu Gly Val Asp Ser  Asp Ser Glu Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Asn Asp Leu Leu Leu  Val Lys Asp Thr Glu  Thr Lys Glu 
    1025                 1030                 1035             


Gln Gln  Leu Phe Arg Phe Leu  Ser Arg Thr Met Pro  Lys Gln Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Asp Lys Gln Lys  Phe Glu Gly 
    1055                 1060                 1065             


Gly Glu  Ala Leu Ile Lys Val  Leu Gly Asn Val Ala  Asn Ser Gly 
    1070                 1075                 1080             


Gln Cys  Lys Lys Gly Leu Gly  Lys Ser Asn Ile Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Val Arg  Thr Asp Val Leu Gly  Asn Gln His Ile Ile  Lys Asn Glu 
    1100                 1105                 1110             


Gly Asp  Lys Pro Lys Leu Asp  Phe 
    1115                 1120     


<210>  672
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  672

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn Arg Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Val Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Arg Thr Asn Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Glu Glu Phe Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
    290                 295                 300                 


Ile Asn Tyr Val Lys Asn Glu Lys Ala Met Gly Pro Ala Lys Leu Phe 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
385                 390                 395                 400 


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
                405                 410                 415     


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
            420                 425                 430         


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Lys Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Ile Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
                485                 490                 495     


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
        515                 520                 525             


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Pro 
            580                 585                 590         


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Phe Asp Val Arg Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
    690                 695                 700                 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Asn 
            740                 745                 750         


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Asp Gln 
        755                 760                 765             


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
    770                 775                 780                 


Glu Ser Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
785                 790                 795                 800 


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
                805                 810                 815     


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
            820                 825                 830         


Ala Lys Val Gly Lys Asp Lys Lys Asp Glu Thr Tyr Val Leu Gly Lys 
        835                 840                 845             


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
865                 870                 875                 880 


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
                885                 890                 895     


Glu Met Asn Glu Lys Gly Lys Glu Val Pro Cys Asn Pro Phe Leu Lys 
            900                 905                 910         


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
        915                 920                 925             


Asp Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Lys Asp Ser Asn Asn Lys Val Val Leu Gln 
945                 950                 955                 960 


Ser Val Ser Pro Trp Arg Ala Asp Val Tyr Phe Asn Lys Thr Thr Gly 
                965                 970                 975     


Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Thr Tyr Lys Ile Ser  Gln Glu Lys Tyr Asn  Asp Ile Lys 
        995                 1000                 1005             


Lys Lys  Glu Gly Val Asp Ser  Asp Ser Glu Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Asn Asp Leu Leu Leu  Val Lys Asp Thr Glu  Thr Lys Glu 
    1025                 1030                 1035             


Gln Gln  Leu Phe Arg Phe Leu  Ser Arg Thr Met Pro  Lys Gln Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Asp Lys Gln Lys  Phe Glu Gly 
    1055                 1060                 1065             


Gly Glu  Ala Leu Ile Lys Val  Leu Gly Asn Val Ala  Asn Ser Gly 
    1070                 1075                 1080             


Gln Cys  Lys Lys Gly Leu Gly  Lys Ser Asn Ile Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Val Arg  Thr Asp Val Leu Gly  Asn Gln His Ile Ile  Lys Asn Glu 
    1100                 1105                 1110             


Gly Asp  Lys Pro Lys Leu Asp  Phe 
    1115                 1120     


<210>  673
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  673

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asn Ser Gly Lys Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Lys 
        35                  40                  45              


Asn Arg Gln Ala Arg Arg Leu His Arg Arg Lys Lys His Arg Gly Val 
    50                  55                  60                  


Arg Phe Gln Asp Leu Phe Glu Asp Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asn Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Thr Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Arg Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Ser Lys Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Cys Leu Lys Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Val Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Glu Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Gly Glu Leu Ser 
        355                 360                 365             


Arg Asn Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr Lys Leu Lys Asp Ser Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys Glu Ile Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys His Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Ser Ile Phe His Gly Gln Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Glu Ser Lys Leu Leu Gly 
                645                 650                 655     


Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Ile Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Ala His 
    690                 695                 700                 


Lys Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Pro Leu Ile Ala Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Glu Thr Gly Glu Ile Ile Ser Leu Ser Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Ser Ser Lys Thr Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Glu Lys Ser Glu Glu Thr Tyr Val Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Gln Ala Gly Tyr Asp Ala Phe Ile Lys 
    850                 855                 860                 


Ile Tyr Asn Lys Asp Lys Ser Lys Phe Leu Met Tyr His Lys Asp Pro 
865                 870                 875                 880 


Gln Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Arg Thr Tyr Pro Ser 
                885                 890                 895     


Lys Glu Leu Asn Asp Lys Asn Lys Glu Ile Pro Cys Asn Pro Phe Glu 
            900                 905                 910         


Lys Tyr Arg Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Ser 
        915                 920                 925             


Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Asn Lys Leu Gly 
    930                 935                 940                 


Asn His Ile Asp Ile Thr Pro Glu Ser Ser Asp Asn Gln Val Val Leu 
945                 950                 955                 960 


Gln Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn His Gln Thr 
                965                 970                 975     


Lys Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu Ser Phe Glu 
            980                 985                 990         


Lys Gly Ser Gly Lys Tyr Ser Ile  Ser Asn Gln Lys Tyr  Asn Ser Ile 
        995                 1000                 1005             


Lys Arg  Ile Glu Gly Ile Asp  Glu Gln Ser Glu Phe  Lys Phe Thr 
    1010                 1015                 1020             


Leu Tyr  Lys Asn Asp Leu Ile  Leu Ile Lys Asp Ser  Glu Asn Asn 
    1025                 1030                 1035             


Glu Gln  Lys Leu Phe Arg Phe  Asn Ser Arg Asn Asp  Thr Ala Lys 
    1040                 1045                 1050             


His Tyr  Ile Glu Leu Lys Pro  Tyr Asp Lys Ala Lys  Phe Asp Gly 
    1055                 1060                 1065             


Glu Gln  Glu Leu Ile Thr Ile  Leu Gly Asn Val Ala  Lys Gly Gly 
    1070                 1075                 1080             


Arg Cys  Leu Lys Gly Leu Asn  Lys Ser Asn Ile Ser  Ile Phe Lys 
    1085                 1090                 1095             


Val Lys  Thr Asp Val Leu Gly  Lys Lys His Ile Ile  Lys Lys Glu 
    1100                 1105                 1110             


Gly Asp  Glu Pro Lys Leu Lys  Phe 
    1115                 1120     


<210>  674
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  674

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Asp Thr Gly Glu Ile Ile His Thr Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Asn 
        35                  40                  45              


Ser Arg Gln Asp Arg Arg Leu Asn Arg Arg Lys Lys His Arg Gly Val 
    50                  55                  60                  


Arg Leu His Asp Leu Phe Glu Asp Tyr Asp Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Gln Gly Leu 
                85                  90                  95      


Asp Asn Ser Leu Thr Thr Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Val Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Lys 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Arg Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Gln Ile Thr 
        195                 200                 205             


Asp Lys Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Glu Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asn Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Thr Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Ser Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Trp Glu Leu Ser 
        355                 360                 365             


Arg Asn Ile Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr Lys Leu Met Asp Val Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Arg Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Val Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Val Ala Ile Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Ser Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Asp Ser Lys Leu Leu Gly 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Thr Leu Gln Asp Phe Tyr Lys Ala His 
    690                 695                 700                 


Lys Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Ala Asn Pro Leu Ile Ser Tyr Lys Glu Asp Gln Phe 
        755                 760                 765             


Val Asp Ser Glu Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Lys Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Ala His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Ser Ser Lys Lys Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Val Lys Leu Asp Lys Asp Lys Ser Glu Glu Thr Tyr Val Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Gln Ala Gly Tyr Asp Ala Phe Ile Lys 
    850                 855                 860                 


Ile Tyr Asn Lys Asp Lys Ser Lys Phe Leu Met Tyr His Lys Asp Pro 
865                 870                 875                 880 


Gln Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Arg Ile Tyr Pro Ser 
                885                 890                 895     


Lys Glu Phe Asn Glu Lys Asn Lys Glu Val Pro Cys Asn Pro Phe Glu 
            900                 905                 910         


Lys Tyr Arg Gln Glu Asn Gly Pro Ile Cys Lys Tyr Ser Lys Lys Gly 
        915                 920                 925             


Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Lys Asn Ile Pro 
    930                 935                 940                 


Glu Ser Phe Ile Asp Ile Thr Pro Ser Asn Ser Lys Asn Lys Val Ile 
945                 950                 955                 960 


Leu Arg Gln Leu Asn Pro Trp Arg Thr Asp Val Tyr Phe Asn His Gln 
                965                 970                 975     


Thr Glu Lys Tyr Glu Leu Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe 
            980                 985                 990         


Glu Lys Lys Thr Glu Thr Tyr Ser  Ile Thr Ile Asp Lys  Tyr Asn Glu 
        995                 1000                 1005             


Ile Lys  Glu Lys Glu Gly Val  Gly Glu His Ser Glu  Phe Lys Phe 
    1010                 1015                 1020             


Thr Leu  Tyr Lys Asn Asp Leu  Leu Leu Ile Lys Asp  Met Glu Ile 
    1025                 1030                 1035             


Lys Glu  Glu Gln Ile Phe Arg  Phe Leu Ser Arg Thr  Met Pro Asn 
    1040                 1045                 1050             


Lys Lys  His Tyr Val Glu Leu  Lys Pro Tyr Tyr Lys  Gly Asn Phe 
    1055                 1060                 1065             


Glu Gly  Lys Glu Arg Ile Lys  Val Phe Asn Thr Thr  Ser Asn Gly 
    1070                 1075                 1080             


Arg Cys  Leu Lys Gly Leu Gly  Lys Lys Gly Leu Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Ile Lys  Thr Asp Val Leu Gly  Asn Lys His Phe Ile  Lys Lys Glu 
    1100                 1105                 1110             


Gly Glu  Lys Pro Lys Leu Lys  Phe 
    1115                 1120     


<210>  675
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  675

Met Asp Arg Thr Glu Val Asn Ile Gly Phe Asp Ile Gly Ile Ser Ser 
1               5                   10                  15      


Val Gly Trp Ser Val Val Ala Thr Lys Ser Gly Arg Ile Leu Glu Ser 
            20                  25                  30          


Gly Val Ser Leu Phe Ser Gly Ala Ser Ala Ala Lys Asn Glu Glu Arg 
        35                  40                  45              


Arg Asn Phe Arg Gln Ser Arg Arg Leu Leu Arg Arg Arg Lys Asn Arg 
    50                  55                  60                  


Ile Ser Asp Leu Lys Lys Tyr Leu Lys Glu Gln Ser Phe Thr Tyr Asn 
65                  70                  75                  80  


Gly Glu Leu Leu Asn Glu Asn Pro Tyr Glu Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Glu Lys Leu Ser Lys Glu Glu Ile Ala Leu Ala Leu Leu His Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Val Ser Tyr Asp Leu Gly Asp Ile Glu Glu Glu 
        115                 120                 125             


Gly Thr Thr Gly Ser Tyr Lys Glu Gly Val Lys Lys Asn Gln Lys Leu 
    130                 135                 140                 


Leu Lys Glu Lys Thr Pro Ala Glu Ile Gln Phe Gln Arg Leu Lys Lys 
145                 150                 155                 160 


Phe His Lys Val Arg Gly Gln Ile Ala Ile Asp Glu Asn Asp Val Leu 
                165                 170                 175     


Leu Asn Val Phe Pro Asn His Ala Tyr Val Asp Glu Ala Gln Gln Ile 
            180                 185                 190         


Leu Tyr Thr Gln Gln Glu Phe Tyr Pro Glu Ile Asn Glu Thr Phe Ile 
        195                 200                 205             


Asn Gln Val Thr Asp Phe Ile Ser Arg Lys Arg Asp Tyr Phe Ile Gly 
    210                 215                 220                 


Pro Gly Ser Glu Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Arg Thr Asp 
225                 230                 235                 240 


Gly Thr Thr Leu Gln Asn Leu Phe Ser Ile Leu Ile Gly Lys Asp Lys 
                245                 250                 255     


Ile Phe Pro Asp Glu Phe Arg Ala Ala Gly Asn Ser Tyr Thr Ala Gln 
            260                 265                 270         


Val Tyr Asn Leu Leu Asn Asp Leu Asn Asn Leu Ala Ile Asp Asp Thr 
        275                 280                 285             


Glu Thr Gly Lys Leu Thr Lys Gln Gln Lys Glu Lys Ile Ile Met Glu 
    290                 295                 300                 


Leu Lys Thr Thr Glu Lys Ser Val Asn Met Met Lys Leu Ile Ala Lys 
305                 310                 315                 320 


Thr Ala Gly Val Thr Val Glu Gln Ile Ser Arg Tyr Arg Ile Asn Arg 
                325                 330                 335     


Asp Gly Lys Ser Glu Ile His Asn Met Ala Val Tyr Arg Lys Ile Arg 
            340                 345                 350         


Lys Val Phe Leu Glu Leu Gly Tyr Glu Ile Asn Glu Trp Pro Ser Gln 
        355                 360                 365             


Leu Leu Asp Glu Leu Gly Asp Ala Leu Ser Leu Asn Thr Glu Asn Gly 
    370                 375                 380                 


Glu Ile Arg Arg Ile Leu Thr Asp Asn Leu Ala Asn Lys Tyr Lys Ile 
385                 390                 395                 400 


Leu Asp Gln Gln Leu Ile Asn Leu Ile Ile Glu Asn Lys Asn Ala Phe 
                405                 410                 415     


Thr Leu Ser Thr Asn Gln Lys Trp His Arg Phe Ser Tyr Lys Thr Met 
            420                 425                 430         


Lys Ile Leu Ile Pro Glu Leu Leu Glu Thr Asn Lys Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Glu Met Gly Leu Met Lys Lys Asp Gln Arg Asn Tyr Val 
    450                 455                 460                 


Gly Lys Asn Lys Ile Asp Ser Glu Ala Leu Leu Glu Asn Leu Tyr Asn 
465                 470                 475                 480 


Pro Val Val Arg Lys Ser Thr Arg Gln Thr Met Lys Leu Phe Asn Thr 
                485                 490                 495     


Leu Leu Lys Lys Tyr Pro Asn Ile Ala Tyr Val Val Ile Glu Met Pro 
            500                 505                 510         


Arg Glu Asp Asp Glu Asp Lys Ala Arg Arg Asp Tyr Val Lys Phe Gln 
        515                 520                 525             


Lys Asp Asn Glu Lys Glu Lys Asp Ala Ala Leu Lys Asn Phe Ile Met 
    530                 535                 540                 


Glu Ser Gly Lys Ser Glu Ala Glu Val Ile Ala Lys Tyr Arg Gln Ser 
545                 550                 555                 560 


Lys Lys Phe Arg Ser Lys Val Arg Leu Trp Tyr Gln Gln Met Gly Arg 
                565                 570                 575     


Cys Leu Tyr Ser Gly Lys Ala Met Pro Ala Glu Arg Val Phe Arg Asp 
            580                 585                 590         


Asp His Phe Thr Asp Ile Asp His Val Ile Pro Leu Ser Val Ser Phe 
        595                 600                 605             


Asp Asp Gly Met Asn Asn Lys Val Leu Cys Leu Ala Glu Met Asn Arg 
    610                 615                 620                 


Gln Lys Asp Lys Gln Thr Pro Tyr Ala Phe Phe Asn Ala Gly His Gly 
625                 630                 635                 640 


Gln Gly Phe Gln Ala Met Asn Ala Met Leu Lys Asn Asn Asn Arg Ile 
                645                 650                 655     


Ser Ser Ala Lys Lys Arg Asn Leu Leu Phe Thr Glu Asn Leu Asn Asp 
            660                 665                 670         


Leu Glu Val Arg Lys Arg Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Glu Leu Gln Gln Phe Val Lys Ala 
    690                 695                 700                 


Lys Gln Leu Asp Thr Lys Val Thr Val Ile Arg Gly Lys Phe Thr Ser 
705                 710                 715                 720 


Lys Leu Arg Glu Lys Trp Asn Ile Asn Lys Ser Arg Asp Thr His His 
                725                 730                 735     


His His Ala Val Asp Ala Ala Val Ile Ala Val Ser Pro Met Leu Asn 
            740                 745                 750         


Leu Trp Glu Lys Gly Ala Ser Ile Ile Pro Asn Lys Val Gly Glu Asn 
        755                 760                 765             


Val Ile Asp Ile Lys Thr Gly Glu Ile Ile Thr Asp His Asp Tyr Glu 
    770                 775                 780                 


Ala Asp Val Tyr Glu Leu Pro Tyr Ala Lys Phe Leu Glu Gln Ile Pro 
785                 790                 795                 800 


Lys Val Lys Lys Asp Ile Lys Phe Arg His Gln Val Asp Lys Lys Lys 
                805                 810                 815     


Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Thr Thr Arg Lys Ala Lys 
            820                 825                 830         


Val Gly Lys Asp Lys Glu Glu Thr Asp Tyr Val Leu Gly Thr Ile Lys 
        835                 840                 845             


Asp Ile Tyr Ser Leu Glu Gly Tyr Val Ser Phe Lys Lys Ile Tyr Asn 
    850                 855                 860                 


Lys Asp Lys Ala Lys Phe Leu Met Ala Arg Ile Asp Pro Lys Thr Phe 
865                 870                 875                 880 


Glu Lys Leu Glu Lys Ile Val Ala Glu Tyr Pro Glu Ser Ile Glu Val 
                885                 890                 895     


Val Gln Asp Asn Gly Lys Val Asp Val Gln Lys Ile Ser Pro Phe Glu 
            900                 905                 910         


Leu Tyr Arg Lys Asp Asn Gly Phe Val Thr Lys Phe Ala Lys Lys Asn 
        915                 920                 925             


Asn Gly Pro Ala Val Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly 
    930                 935                 940                 


Ser Ser Ile Asp Val Thr Pro Asp Asn Ala Lys Asn Lys Lys Val Ile 
945                 950                 955                 960 


Leu Gln Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn Glu Glu 
                965                 970                 975     


Asn Gln Glu Tyr Glu Ile Met Gly Leu Lys Tyr Ser Asp Leu Lys Phe 
            980                 985                 990         


Gly Lys Asn Gly Lys Tyr Gly Val  Pro Lys Val Arg Tyr  Glu Glu Ile 
        995                 1000                 1005             


Lys Gln  Glu Glu Glu Ile Ser  Glu Gly Ser Lys Phe  Leu Phe Ser 
    1010                 1015                 1020             


Leu Tyr  Arg Gly Asp Arg Ile  Lys Val Thr Asn Glu  Leu Gly Glu 
    1025                 1030                 1035             


Asn Leu  Asp Leu Leu Phe Ser  Ser Arg Thr Leu Pro  Ala Gln Lys 
    1040                 1045                 1050             


Gly Tyr  Val Glu Leu Lys Pro  Ile Asp Lys Thr Lys  Phe Asp Gly 
    1055                 1060                 1065             


Lys Glu  Lys Val Ser Phe Tyr  Gly Thr Val Thr Pro  Asn Gly Gln 
    1070                 1075                 1080             


Phe Val  Lys Lys Phe Ala Lys  Lys Gly Tyr Lys Ile  Leu Lys Leu 
    1085                 1090                 1095             


Asn Thr  Asp Glu Leu Gly Asn  Thr Tyr Ile Glu Asn  Gly Glu Lys 
    1100                 1105                 1110             


Glu Pro  Ser Asp Ile Leu Asp  Met 
    1115                 1120     


<210>  676
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  676

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Asp Thr Gly Glu Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Val Arg Arg Ser 
        35                  40                  45              


Ser Arg Gln Gly Arg Arg Leu Asn His Arg Lys Lys His Arg Ser Val 
    50                  55                  60                  


Arg Leu Lys Asp Leu Phe Glu Tyr Tyr Asp Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asn Gly Leu 
                85                  90                  95      


Asn Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Ala Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Tyr Asn Asn Gln Ile Met 
        195                 200                 205             


Asp Glu Phe Ile Ala Asp Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Glu Lys Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Thr Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Ser Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Lys Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Gly Glu Phe Ser 
        355                 360                 365             


Arg Glu Val Leu Asp Glu Leu Ala His Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr Lys Leu Arg Asp Ser Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Ile Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Ala Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Phe Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Gln Glu Val Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Glu Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn His Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Glu Lys Glu Leu Pro Asp Ser Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Val Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Asp Ser Lys Ser Leu Gly 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Thr Leu Gln Asp Phe Tyr Lys Ala His 
    690                 695                 700                 


Lys Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Pro Leu Ile Ser Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Glu Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Arg Ser Lys Lys Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Thr Lys Leu Asp Lys Asp Lys Ser Glu Glu Thr Tyr Val Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Gln Ser Gly Tyr Asp Ala Phe Ile Lys 
    850                 855                 860                 


Ile Tyr Asn Lys Asp Lys Ser Lys Phe Leu Met Tyr His Lys Asp Pro 
865                 870                 875                 880 


Gln Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Arg Thr Tyr Pro Ser 
                885                 890                 895     


Lys Glu Phe Asn Glu Lys Asn Lys Glu Val Ser Cys Asn Pro Phe Glu 
            900                 905                 910         


Lys Tyr Lys Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly 
        915                 920                 925             


Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Lys Asn Ile Pro 
    930                 935                 940                 


Glu Ser Phe Ile Asn Ile Thr Pro Ser Asn Ser Lys Asn Lys Val Ile 
945                 950                 955                 960 


Leu Arg Gln Leu Asn Pro Trp Arg Thr Asp Val Tyr Phe Asn His Gln 
                965                 970                 975     


Thr Glu Lys Tyr Glu Leu Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe 
            980                 985                 990         


Glu Lys Lys Thr Gly Thr Tyr Ser  Ile Thr Ile Asp Lys  Tyr Asn Glu 
        995                 1000                 1005             


Ile Lys  Glu Lys Glu Gly Val  Ser Glu His Ser Glu  Phe Lys Phe 
    1010                 1015                 1020             


Thr Leu  Tyr Lys Asn Asp Leu  Leu Leu Ile Lys Asp  Thr Glu Ile 
    1025                 1030                 1035             


Lys Glu  Glu Gln Ile Phe Arg  Phe Leu Ser Arg Thr  Met Pro Asn 
    1040                 1045                 1050             


Gln Lys  His Tyr Val Glu Leu  Lys Pro Tyr Tyr Lys  Gly Asn Phe 
    1055                 1060                 1065             


Glu Gly  Lys Glu Arg Ile Lys  Val Phe Asn Thr Thr  Ser Asn Gly 
    1070                 1075                 1080             


Arg Cys  Leu Lys Gly Leu Arg  Lys Lys Gly Leu Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Ile Lys  Thr Asp Val Leu Gly  Asn Lys His Phe Ile  Lys Lys Glu 
    1100                 1105                 1110             


Gly Glu  Lys Pro Lys Leu Lys  Phe 
    1115                 1120     


<210>  677
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  677

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Ile Val 
    50                  55                  60                  


Arg Leu Asn Arg Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Arg Thr Ser Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Phe Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
    290                 295                 300                 


Ile Asn Tyr Val Lys Asn Glu Lys Ala Met Gly Pro Ala Lys Leu Phe 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Glu 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
385                 390                 395                 400 


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
                405                 410                 415     


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
            420                 425                 430         


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
                485                 490                 495     


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
        515                 520                 525             


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Ser 
            580                 585                 590         


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Phe Asp Val Arg Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
    690                 695                 700                 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ile Gln Leu Asn 
            740                 745                 750         


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Asp Gln 
        755                 760                 765             


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
    770                 775                 780                 


Glu Ser Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
785                 790                 795                 800 


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
                805                 810                 815     


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
            820                 825                 830         


Ala Lys Val Gly Lys Asp Lys Ala Asp Glu Thr Tyr Val Leu Gly Lys 
        835                 840                 845             


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
865                 870                 875                 880 


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
                885                 890                 895     


Gln Ile Asn Glu Lys Gly Lys Glu Val Pro Val Asn Pro Phe Leu Lys 
            900                 905                 910         


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
        915                 920                 925             


Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Lys Asp Ser Asn Asn Lys Val Val Leu Gln 
945                 950                 955                 960 


Ser Val Ser Pro Trp Arg Ala Asp Val Tyr Phe Asn Lys Thr Thr Gly 
                965                 970                 975     


Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Thr Tyr Lys Ile Ser  Gln Glu Lys Tyr Asn  Asp Ile Lys 
        995                 1000                 1005             


Lys Lys  Glu Gly Val Asp Ser  Asp Ser Glu Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Asn Asp Leu Leu Leu  Val Lys Asp Thr Glu  Thr Lys Glu 
    1025                 1030                 1035             


Gln Gln  Leu Phe Arg Phe Leu  Ser Arg Thr Met Pro  Lys Gln Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Asp Lys Gln Lys  Phe Glu Gly 
    1055                 1060                 1065             


Gly Glu  Ala Leu Ile Lys Val  Leu Gly Asn Val Ala  Asn Ser Gly 
    1070                 1075                 1080             


Gln Cys  Lys Lys Gly Leu Gly  Lys Ser Asn Ile Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Val Arg  Thr Asp Val Leu Gly  Asn Gln His Ile Ile  Lys Asn Glu 
    1100                 1105                 1110             


Gly Asp  Lys Pro Lys Leu Asp  Phe 
    1115                 1120     


<210>  678
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  678

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asn Ser Gly Lys Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Lys 
        35                  40                  45              


Asn Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Lys His Arg Gly Val 
    50                  55                  60                  


Arg Phe Gln Asp Leu Phe Glu Asp Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asn Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Ala Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Cys Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Arg Lys Glu 
            180                 185                 190         


Ala Glu Lys Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Val Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asn Glu Tyr Arg Ala Ser Lys Ser Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Val Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Glu Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Val Lys Met Ile Asp Ala Ser Val Asp Gln Ile Ser Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Gly Glu Leu Ser 
        355                 360                 365             


Arg Asn Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Ser Lys Leu Met Gly Val Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys His Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Gly Asn Ile Phe Asn Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Thr Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val His Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Asp Leu Lys Ser Leu Ser 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Ser His 
    690                 695                 700                 


Gln Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Pro Leu Ile Thr Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Gln Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


His Ser Lys Thr Phe Glu Asp Gly Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Asp Lys Ser Glu Glu Thr Tyr Val Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Gln Ala Gly Tyr Asp Ala Phe Ile Lys 
    850                 855                 860                 


Ile Tyr Asn Lys Asp Lys Arg Lys Phe Leu Met Tyr His Lys Asp Pro 
865                 870                 875                 880 


Gln Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Arg Thr Tyr Pro Ser 
                885                 890                 895     


Lys Glu Arg Asn Asp Lys Asn Lys Glu Val Pro Cys Asn Pro Phe Glu 
            900                 905                 910         


Lys Tyr Arg Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly 
        915                 920                 925             


Asn Gly Pro Glu Ile Lys Cys Leu Lys Tyr Tyr Asp Asn Lys Leu Gly 
    930                 935                 940                 


Asn Tyr Ile Asp Ile Ser Pro Asp Gly Ser Asp Asn Gln Val Val Leu 
945                 950                 955                 960 


Gln Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn His Gln Thr 
                965                 970                 975     


Lys Glu Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu Ser Phe Glu 
            980                 985                 990         


Lys Gly Ser Gly Lys Tyr Ser Ile  Ser Asn Glu Lys Tyr  Asn Ser Ile 
        995                 1000                 1005             


Lys Arg  Ile Glu Gly Val Asn  Glu Gln Ser Glu Phe  Lys Phe Thr 
    1010                 1015                 1020             


Leu Tyr  Lys Asn Asp Leu Ile  Leu Ile Lys Asp Ser  Glu Asn Asn 
    1025                 1030                 1035             


Glu Gln  Lys Leu Phe Arg Phe  Asn Ser Arg Asn Asp  Thr Ala Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Asp Lys Ala Arg  Phe Asp Gly 
    1055                 1060                 1065             


Gln Gln  Glu Leu Ile Thr Ile  Leu Gly Asn Val Ala  Lys Gly Gly 
    1070                 1075                 1080             


Gln Cys  Leu Lys Gly Leu Asn  Lys Ser Asn Ile Ser  Ile Phe Lys 
    1085                 1090                 1095             


Val Lys  Thr Asp Val Leu Gly  Lys Lys His Ile Ile  Lys Lys Glu 
    1100                 1105                 1110             


Gly Asp  Glu Pro Lys Leu Lys  Phe 
    1115                 1120     


<210>  679
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  679

Met Ala Asp Lys Thr Leu Gly Ala Leu Thr Phe Gly Phe Asp Ile Gly 
1               5                   10                  15      


Ile Ala Ser Val Gly Trp Cys Val Leu Gly Glu Asn Arg Ile Val Asp 
            20                  25                  30          


Leu Gly Val Arg Ala Phe Asp Lys Ala Glu Thr Ala Lys Glu Gly Glu 
        35                  40                  45              


Ser Leu Asn Leu Ala Arg Arg Thr Ala Arg Leu Met Arg Arg Arg Leu 
    50                  55                  60                  


Arg Arg Arg Ala Trp Arg Leu Thr Lys Leu Ala Arg Leu Leu Lys Arg 
65                  70                  75                  80  


Glu Gly Leu Ile Ala Asp Ala Phe Gln Met His Gln Pro Ser Ala Thr 
                85                  90                  95      


Ser Pro Trp Lys Leu Arg Val Glu Ala Leu Asp Arg Arg Leu Thr Asp 
            100                 105                 110         


Asp Glu Trp Ala Arg Val Ile Tyr His Leu Cys Lys His Arg Gly Phe 
        115                 120                 125             


His Trp Ile Ser Arg Ala Glu Glu Lys Lys Ala Glu Gly Asp Ala Lys 
    130                 135                 140                 


Gly Glu Ser Gly Arg Val Lys Lys Gly Leu Ser Asp Thr Gln Arg Arg 
145                 150                 155                 160 


Met Gly Glu Lys Gly Tyr Arg Ser Ala Ala Glu Met Val Leu Ala Glu 
                165                 170                 175     


Phe Pro Glu Ala Gln Arg Asn Lys Gln Gly Asp Tyr Gly Lys Ala Leu 
            180                 185                 190         


Ser Arg Val Leu Leu Ser Glu Glu Leu Ala Leu Leu Phe Arg Arg Gln 
        195                 200                 205             


Arg Glu Leu Gly His Thr His Ala Ser Lys Glu Leu Glu Ala Ala Ile 
    210                 215                 220                 


Leu Gly Asn Gly Asp Arg Lys Gly Gly Leu Phe Trp Ala Gln Lys Pro 
225                 230                 235                 240 


Pro Leu Ala Gly Ala Asp Leu Leu Lys Met Leu Gly Lys Cys Thr Phe 
                245                 250                 255     


Glu Lys Asp Glu Tyr Arg Ala Pro Lys Ala Ser Phe Thr Ala Glu Arg 
            260                 265                 270         


His Val Trp Leu Thr Arg Leu Asn Asn Leu Arg Val Val Val Asp Gly 
        275                 280                 285             


Thr Thr Arg Pro Leu Thr Glu Asp Glu Arg Arg Val Ala Leu Pro Leu 
    290                 295                 300                 


Pro Tyr Gly Tyr Ala Asp Ala Lys Leu Thr Tyr Lys Gln Leu Arg Ala 
305                 310                 315                 320 


Ala Leu Ser Lys Ala Gly Leu Ser Asn Phe Ser Phe Ala Gly Ile Ala 
                325                 330                 335     


Asp Asp Lys Glu Lys Asp Thr Leu Thr Lys Leu Pro Ala Trp Glu Thr 
            340                 345                 350         


Leu Ser Lys Thr Leu Lys Gly Lys Gly Leu Glu Thr Glu Trp Glu Gly 
        355                 360                 365             


Met Ala Gly Ala Ala Thr Gly Gly Asp Pro Thr Leu Leu Asp Glu Ile 
    370                 375                 380                 


Ser Arg Val Leu Ser Val Tyr Lys Asp Gly Asp Glu Val Asp Val Glu 
385                 390                 395                 400 


Leu Arg Lys Leu Pro Leu Pro Gly Gly Glu Lys Met Val Glu Thr Leu 
                405                 410                 415     


Ser Glu Ile Ser Phe Asp Lys Phe His Ala Leu Ser Leu Lys Ala Leu 
            420                 425                 430         


Arg Lys Ile Val Pro His Met Glu Thr Gly Leu Arg Tyr Asp Glu Ala 
        435                 440                 445             


Cys Ala Ala Ala Gly Tyr His His Ser Gln Leu His Glu Ala Gly Ala 
    450                 455                 460                 


Gly Gly Gln Lys Tyr Leu Pro Pro Phe Tyr Thr Arg Arg Asp Lys Asp 
465                 470                 475                 480 


Gly Arg Met Val Phe Asp Glu Glu Leu Asp Val Pro Arg Asn Pro Val 
                485                 490                 495     


Val Leu Arg Ala Leu Asn Gln Ala Arg Lys Val Leu Asn Ala Leu Val 
            500                 505                 510         


Arg Ala Tyr Gly Ser Pro Ser Ala Val His Ile Glu Met Ala Arg Asp 
        515                 520                 525             


Leu Ser Lys Pro Leu Val Gly His Pro Thr Pro Asn Gly Gly Tyr Ile 
    530                 535                 540                 


Glu Gly Arg Arg Asp Ile Glu Lys Leu Gln Lys Glu Tyr Arg Glu Arg 
545                 550                 555                 560 


Asn Glu Lys Asp Lys Ala Ala Phe Ala Ala Glu His Gly Ile Ala Ala 
                565                 570                 575     


Lys Ser Arg Glu Phe Glu Lys Phe Gln Leu Tyr Arg Glu Gln Gln Gly 
            580                 585                 590         


Lys Cys Ala Tyr Ser Leu Lys Pro Leu Asp Leu His Arg Val Leu His 
        595                 600                 605             


Asp Ile Gly Tyr Ala Glu Val Asp His Ala Leu Pro Tyr Ser Arg Ser 
    610                 615                 620                 


Tyr Asp Asp Gly Lys Asn Asn Lys Val Leu Val Leu Thr Glu Glu Asn 
625                 630                 635                 640 


Arg Asn Lys Gly Asn Arg Thr Ala Tyr Glu Tyr Leu Cys Ala Phe Pro 
                645                 650                 655     


Gly Gly Glu Asn Gly Glu Arg Trp Arg Asn Phe Val Ala Tyr Val Glu 
            660                 665                 670         


Ser Asn Lys Ser Tyr Arg Leu Ala Lys Arg Thr Arg Leu Leu Arg Lys 
        675                 680                 685             


Asp Phe Gly Gly Lys Ala Ala Glu Glu Phe Arg Glu Arg Asn Leu Asn 
    690                 695                 700                 


Asp Thr Arg Tyr Ile Cys Lys Phe Phe Lys Asn Phe Val Glu Arg Phe 
705                 710                 715                 720 


Leu Gln Leu Lys Val Gly Ala Asp Gly Thr Ala Ala Lys Arg Cys Val 
                725                 730                 735     


Val Leu Ser Gly Gln Thr Thr Ala Phe Leu Arg Ala Arg Trp Gly Leu 
            740                 745                 750         


Leu Lys Val Arg Gly Ala Ser Asp Arg His His Ala Leu Asp Ala Ala 
        755                 760                 765             


Val Val Ala Ala Cys Ser His Gly Met Val Lys Arg Leu Ala Asp Tyr 
    770                 775                 780                 


Ser Arg Thr Lys Glu Leu Ala Lys Val Arg Asp Gly Phe Pro Asp Pro 
785                 790                 795                 800 


Glu Thr Gly Glu Ile Val Asp Pro Ala Met Phe Gln Gln Leu His Ala 
                805                 810                 815     


His Phe Pro Asp Pro Trp Pro His Phe Arg Gly Glu Leu Glu Ala Arg 
            820                 825                 830         


Leu Gln Leu Asp Asp Ser Ala Glu Leu Arg Ala Gln Leu Glu Met Phe 
        835                 840                 845             


Gly Thr Tyr Pro Ala Glu Ala Leu Glu Thr Leu Arg Pro Leu Phe Val 
    850                 855                 860                 


Ser Arg Ala Pro Gln Arg Arg Asn Gly Gly Ala Ala His Lys Glu Thr 
865                 870                 875                 880 


Ile Tyr Ala Gln Pro Glu Arg Leu Lys Glu Gln Gly Gly Val Thr Gln 
                885                 890                 895     


Lys Val Ser Leu Ala Ser Leu Lys Pro Thr Asp Ile Asp Lys Leu Ile 
            900                 905                 910         


Asp Pro His Arg Asn Glu Lys Leu Tyr Ala Ala Ile Arg Ala Arg Leu 
        915                 920                 925             


Glu Ala His Gly Gly Lys Ser Asp Lys Ala Phe Pro Ala Asp Asn Pro 
    930                 935                 940                 


Leu Arg Lys Pro Asp Arg Asp Gly Asn Pro Thr Gly Pro Ile Val Arg 
945                 950                 955                 960 


Ala Val Thr Met Val Ile Asp Lys Leu Ser Gly Ile Gln Ile Arg Gly 
                965                 970                 975     


Gly Ile Ala Lys Asn Asp Thr Met Leu Arg Val Asp Val Phe Arg His 
            980                 985                 990         


Lys Lys Asp Gly Lys Phe His Leu  Val Pro Leu Tyr Val  His His Ala 
        995                 1000                 1005             


Val Ala  Gln Glu Leu Pro Asn  Arg Ala Ile Val Ala  Phe Lys Asp 
    1010                 1015                 1020             


Glu Asp  Glu Trp Thr Leu Ile  Asp Asp Asn Phe His  Phe Leu Phe 
    1025                 1030                 1035             


Ser Phe  His Pro Asn Asp Phe  Ile Arg Ile Thr Gln  Lys Asn Lys 
    1040                 1045                 1050             


Ala Pro  Ile Leu Gly Tyr Tyr  Ala Ser Cys His Arg  Gly Thr Gly 
    1055                 1060                 1065             


Asn Leu  Asn Leu Trp Ala His  Asp Arg Asn Gln Lys  Val Gly Lys 
    1070                 1075                 1080             


Asp Gly  Gln Ile Glu Gly Ile  Gly Val Lys Thr Ala  Val Ala Leu 
    1085                 1090                 1095             


Glu Lys  Phe His Val Asp Val  Leu Gly Asn Ile Tyr  Pro Ala Pro 
    1100                 1105                 1110             


Pro Glu  Gln Arg His Gly Leu  Ala 
    1115                 1120     


<210>  680
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  680

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn Arg Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Arg Thr Ser Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Phe Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
    290                 295                 300                 


Ile Asn Tyr Val Lys Asn Glu Lys Ala Met Gly Pro Ala Lys Leu Phe 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
385                 390                 395                 400 


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
                405                 410                 415     


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
            420                 425                 430         


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
                485                 490                 495     


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
        515                 520                 525             


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Ser 
            580                 585                 590         


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Phe Asp Val Arg Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
    690                 695                 700                 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Asn 
            740                 745                 750         


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Asp Gln 
        755                 760                 765             


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
    770                 775                 780                 


Glu Ser Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
785                 790                 795                 800 


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
                805                 810                 815     


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
            820                 825                 830         


Ala Lys Val Gly Lys Asp Lys Ala Asp Glu Thr Tyr Val Leu Gly Lys 
        835                 840                 845             


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
865                 870                 875                 880 


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
                885                 890                 895     


Gln Ile Asn Glu Lys Gly Lys Glu Val Pro Cys Asn Pro Phe Leu Lys 
            900                 905                 910         


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
        915                 920                 925             


Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Lys Asp Ser Asn Asn Lys Val Val Leu Gln 
945                 950                 955                 960 


Ser Val Ser Pro Trp Arg Ala Asp Val Tyr Phe Asn Lys Thr Thr Gly 
                965                 970                 975     


Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Thr Tyr Lys Ile Ser  Gln Glu Lys Tyr Asn  Asp Ile Lys 
        995                 1000                 1005             


Lys Lys  Glu Gly Val Asp Ser  Asp Ser Glu Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Asn Asp Leu Leu Leu  Val Lys Asp Thr Glu  Thr Lys Glu 
    1025                 1030                 1035             


Gln Gln  Leu Phe Arg Phe Leu  Ser Arg Thr Met Pro  Lys Gln Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Asp Lys Gln Lys  Phe Glu Gly 
    1055                 1060                 1065             


Gly Glu  Ala Leu Ile Lys Val  Leu Gly Asn Val Ala  Asn Ser Gly 
    1070                 1075                 1080             


Gln Cys  Lys Lys Gly Leu Gly  Lys Ser Asn Ile Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Val Arg  Thr Asp Val Leu Gly  Asn Gln His Ile Ile  Lys Asn Glu 
    1100                 1105                 1110             


Gly Asp  Lys Pro Lys Leu Asp  Phe 
    1115                 1120     


<210>  681
<211>  1121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  681

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Asp Thr Gly Glu Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Val Arg Arg Ser 
        35                  40                  45              


Ser Arg Gln Gly Arg Arg Leu Asn His Arg Lys Lys His Arg Ser Val 
    50                  55                  60                  


Arg Leu Lys Asp Leu Phe Glu Tyr Tyr Asp Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asn Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Ala Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Tyr Asn Asn Gln Ile Met 
        195                 200                 205             


Asp Glu Phe Ile Ala Asp Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Thr Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Ser Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Leu Leu Glu Thr Ile Pro Val Gly Glu Phe Ser 
        355                 360                 365             


Arg Glu Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr Lys Leu Met Asp Val Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Ile Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Ile 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Ala Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Phe Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Gln Glu Val Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Glu Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn His Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Glu Lys Glu Leu Pro Asp Ser Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Val Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Arg Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Asp Ser Lys Ser Leu Gly 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Thr Leu Gln Asp Phe Tyr Lys Ala His 
    690                 695                 700                 


Lys Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Pro Leu Ile Ser Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Glu Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Arg Ser Lys Lys Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Asp Lys Ser Glu Glu Thr Tyr Val Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Gln Ser Gly Tyr Asp Ala Phe Ile Lys 
    850                 855                 860                 


Ile Tyr Asn Lys Asp Lys Ser Lys Phe Leu Met Tyr His Lys Asp Pro 
865                 870                 875                 880 


Gln Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Arg Thr Tyr Pro Ser 
                885                 890                 895     


Lys Glu Phe Asn Glu Lys Asn Lys Glu Val Ser Cys Asn Pro Phe Glu 
            900                 905                 910         


Lys Tyr Lys Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly 
        915                 920                 925             


Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Lys Asn Ile Pro 
    930                 935                 940                 


Glu Ser Phe Ile Asn Ile Thr Pro Ser Asn Ser Lys Asn Lys Val Ile 
945                 950                 955                 960 


Leu Arg Gln Leu Asn Pro Trp Arg Thr Asp Val Tyr Phe Asn His Gln 
                965                 970                 975     


Thr Glu Lys Tyr Glu Leu Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe 
            980                 985                 990         


Glu Lys Lys Thr Gly Thr Tyr Ser  Ile Thr Ile Asp Lys  Tyr Asn Glu 
        995                 1000                 1005             


Ile Lys  Glu Lys Glu Gly Val  Ser Glu His Ser Glu  Phe Lys Phe 
    1010                 1015                 1020             


Thr Leu  Tyr Lys Asn Asp Leu  Leu Leu Ile Lys Asp  Thr Glu Ile 
    1025                 1030                 1035             


Lys Glu  Glu Gln Ile Phe Arg  Phe Leu Ser Arg Thr  Met Pro Asn 
    1040                 1045                 1050             


Gln Lys  His Tyr Val Glu Leu  Lys Pro Tyr Tyr Lys  Gly Asn Phe 
    1055                 1060                 1065             


Glu Gly  Lys Glu Arg Ile Lys  Val Phe Asn Thr Thr  Ser Asn Gly 
    1070                 1075                 1080             


Arg Cys  Leu Lys Gly Leu Arg  Lys Lys Gly Leu Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Ile Lys  Thr Asp Val Leu Gly  Asn Lys His Phe Ile  Lys Lys Glu 
    1100                 1105                 1110             


Gly Glu  Lys Pro Lys Leu Lys  Phe 
    1115                 1120     


<210>  682
<211>  1122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  682

Met Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Val Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asp Lys Lys Thr Gly Glu Ile Ile His Ala Ser Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Ser Asn Val Glu Arg Arg Gly 
        35                  40                  45              


Phe Arg Gln Gly Arg Arg Leu Gly Arg Arg Lys Lys His Arg Lys Val 
    50                  55                  60                  


Arg Leu Ala Asp Leu Phe Ser Asp Thr Gly Leu Ile Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Glu Leu Arg Ile Lys Gly Leu 
                85                  90                  95      


Asn Glu Lys Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Asn Glu Asp Gly 
        115                 120                 125             


Glu Ser Ser Ser Ser Glu Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Asp Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Ile Glu Glu Asn Gly Glu 
                165                 170                 175     


Lys His Arg Leu Leu Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Thr Lys Gln Gln Asp Tyr Asn Gln Asp Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Gln Ala Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Arg Gln Ile 
    290                 295                 300                 


Ile Glu Tyr Ala Lys Gly Ala Lys Thr Leu Gly Ala Ala Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Val Asp Gly Ser Val Glu Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Glu Lys Pro Glu Met His Thr Phe Asp Ile 
            340                 345                 350         


Tyr Arg Lys Met Gln Thr Leu Glu Thr Val Asp Val Glu Lys Leu Ser 
        355                 360                 365             


Arg Glu Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Lys Val Ser Phe Ile Lys Arg Glu 
385                 390                 395                 400 


Phe Glu Gln Asp Gln Ile Ala Glu Leu Val Ser Phe Arg Lys Ser Asn 
                405                 410                 415     


Ser Ser Leu Phe Gly Lys Gly Trp His Asn Phe Ser Ile Lys Leu Met 
            420                 425                 430         


Thr Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Lys Ala Arg Ser Lys Arg 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Glu Leu Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Leu Ala 
                485                 490                 495     


Thr Lys Lys Tyr Gly Val Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Val Lys Arg Gln Lys 
        515                 520                 525             


Ala Asn Glu Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala His Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Lys Glu Leu Pro Asp Asn Val Phe His Gly His Lys 
545                 550                 555                 560 


Glu Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp Leu Ile His Asn Gln 
            580                 585                 590         


Tyr Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Tyr Arg Glu Phe Lys Ala Tyr Val Arg Gly Ala Arg Ala Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Asp Tyr Leu Leu Asn Glu Glu Asp Ile Asn Lys 
            660                 665                 670         


Ile Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Leu 
    690                 695                 700                 


Asn Asp Phe Asp Thr Lys Ile Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Arg Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg 
            740                 745                 750         


Leu Trp Lys Lys Gln Gly Asn Pro Leu Ile Ser Tyr Lys Glu Asn Gln 
        755                 760                 765             


Phe Val Asp Ser Glu Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Glu 
    770                 775                 780                 


Tyr Lys Glu Leu Val Phe Arg Ala Pro Tyr Asp His Phe Val Asp Thr 
785                 790                 795                 800 


Val Ser Ser Lys Lys Phe Glu Asp Arg Ile Leu Phe Ser Tyr Gln Val 
                805                 810                 815     


Asp Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ser Thr 
            820                 825                 830         


Arg Lys Ala Lys Leu Gly Lys Asp Lys Ser Glu Glu Thr Tyr Val Leu 
        835                 840                 845             


Gly Lys Ile Lys Asp Ile Tyr Thr Gln Thr Gly Tyr Asp Ala Phe Ile 
    850                 855                 860                 


Lys Leu Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr His Lys Asp 
865                 870                 875                 880 


Pro Ile Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Lys Thr Tyr Pro 
                885                 890                 895     


Asp Lys Glu Ile Asn Glu Lys Gly Lys Glu Val Ala Cys Asn Pro Phe 
            900                 905                 910         


Glu Lys Tyr Arg Gln Glu Asn Gly Pro Leu Arg Lys Tyr Ser Lys Lys 
        915                 920                 925             


Gly Lys Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Asn Lys Leu 
    930                 935                 940                 


Gly Asn His Ile Asp Ile Thr Pro Asp Asn Ser Glu Asn Gln Val Ile 
945                 950                 955                 960 


Leu Gln Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn His Lys 
                965                 970                 975     


Thr Lys Ile Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu Ser Phe 
            980                 985                 990         


Glu Lys Gly Ser Gly Lys Tyr Arg  Ile Ser Leu Asp Lys  Tyr Asn Val 
        995                 1000                 1005             


Ile Lys  Lys Lys Glu Gly Val  His Lys Glu Ser Glu  Phe Lys Phe 
    1010                 1015                 1020             


Thr Leu  Tyr Lys Asn Asp Leu  Ile Leu Ile Lys Asp  Leu Glu Lys 
    1025                 1030                 1035             


Ser Glu  Gln Gln Leu Phe Arg  Tyr Asn Ser Arg Asn  Asp Thr Ser 
    1040                 1045                 1050             


Lys His  Tyr Val Glu Leu Lys  Pro Tyr Asp Lys Ala  Lys Phe Glu 
    1055                 1060                 1065             


Gly Asn  Gln Pro Leu Met Ala  Leu Phe Gly Asn Val  Ala Lys Gly 
    1070                 1075                 1080             


Gly Gln  Cys Leu Lys Gly Leu  Asn Lys Ala Asn Ile  Ser Ile Tyr 
    1085                 1090                 1095             


Lys Val  Gln Thr Asp Val Leu  Gly Asn Lys Arg Phe  Ile Lys Lys 
    1100                 1105                 1110             


Glu Gly  Asp Ala Pro Lys Leu  Glu Phe 
    1115                 1120         


<210>  683
<211>  1122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  683

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Ile Val 
    50                  55                  60                  


Arg Leu Asn Arg Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Ser Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Arg Thr Asn Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Phe Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
    290                 295                 300                 


Ile Asn Tyr Val Lys Asn Glu Lys Val Met Gly Pro Ala Lys Leu Phe 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Gly 
                325                 330                 335     


His Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
            340                 345                 350         


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
385                 390                 395                 400 


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
                405                 410                 415     


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
            420                 425                 430         


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
                485                 490                 495     


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
        515                 520                 525             


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Pro 
            580                 585                 590         


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Phe Asp Val Arg Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
    690                 695                 700                 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Asn 
            740                 745                 750         


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Glu Gln 
        755                 760                 765             


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
    770                 775                 780                 


Glu Ser Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
785                 790                 795                 800 


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
                805                 810                 815     


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
            820                 825                 830         


Ala Lys Val Gly Lys Asp Lys Lys Asp Glu Thr Tyr Val Leu Gly Lys 
        835                 840                 845             


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
    850                 855                 860                 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
865                 870                 875                 880 


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
                885                 890                 895     


Glu Met Asn Glu Lys Gly Lys Glu Val Pro Cys Asn Pro Phe Leu Lys 
            900                 905                 910         


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
        915                 920                 925             


Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Leu Gly 
    930                 935                 940                 


Asn Pro Ile Asp Ile Thr Pro Glu Asn Ser Lys Asn Lys Val Val Leu 
945                 950                 955                 960 


Gln Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn Lys Asn Thr 
                965                 970                 975     


Gly Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe Glu 
            980                 985                 990         


Lys Lys Thr Gly Thr Tyr Lys Ile  Ser Gln Glu Lys Tyr  Asn Gly Ile 
        995                 1000                 1005             


Met Lys  Glu Glu Gly Val Asp  Ser Asp Ser Glu Phe  Lys Phe Thr 
    1010                 1015                 1020             


Leu Tyr  Lys Asn Asp Leu Leu  Leu Val Lys Asp Thr  Glu Thr Lys 
    1025                 1030                 1035             


Glu Gln  Gln Leu Phe Arg Phe  Leu Ser Arg Thr Met  Pro Asn Val 
    1040                 1045                 1050             


Lys Tyr  Tyr Val Glu Leu Lys  Pro Tyr Ser Lys Asp  Lys Phe Glu 
    1055                 1060                 1065             


Lys Asn  Glu Ser Leu Ile Glu  Ile Leu Gly Ser Ala  Asp Lys Ser 
    1070                 1075                 1080             


Gly Arg  Cys Ile Lys Gly Leu  Gly Lys Ser Asn Ile  Ser Ile Tyr 
    1085                 1090                 1095             


Lys Val  Arg Thr Asp Val Leu  Gly Asn Gln His Ile  Ile Lys Asn 
    1100                 1105                 1110             


Glu Gly  Asp Lys Pro Lys Leu  Asp Phe 
    1115                 1120         


<210>  684
<211>  1122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  684

Met Ser Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Val Ile Asp Ala Gln Thr Gly Glu Ile Ile His Ala Ser 
            20                  25                  30          


Ser Arg Ile Phe Pro Ser Ala Asn Ala Ala Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Thr Phe Arg Gly Ser Arg Arg Leu Ile Arg Arg Lys Lys His Arg Ile 
    50                  55                  60                  


Lys Arg Leu Asp Asp Leu Phe Asn Asp Phe His Ile Asn Leu Asp Gly 
65                  70                  75                  80  


Glu Met Ser Thr Asp Asn Pro Tyr Val Leu Arg Val Lys Gly Leu Ser 
                85                  90                  95      


Gln Lys Leu Thr Val Glu Glu Leu Tyr Ile Ser Ile Lys Asn Ile Met 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Ser Asp Asn Glu 
        115                 120                 125             


Ala Gly Arg Ser Asp Tyr Ala Lys Ala Ile Glu Arg Asn Arg Gln Leu 
    130                 135                 140                 


Leu Thr Ser Lys Thr Pro Gly Glu Ile Gln Leu Glu Arg Leu Glu Lys 
145                 150                 155                 160 


Tyr Gly Gln Leu Arg Gly Asn Phe Thr Ile Ile Asp Glu Glu Gly Gln 
                165                 170                 175     


Ser Gln Gln Ile Ile Asn Val Phe Ser Thr Ser Asp Tyr Val Lys Glu 
            180                 185                 190         


Val Glu Lys Ile Leu Asp Cys Gln Lys Met Tyr His Lys Phe Ile Ser 
        195                 200                 205             


Asp Glu Phe Cys Asp Lys Leu Ile Glu Leu Leu Arg Glu Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr Val Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Ile 
225                 230                 235                 240 


Tyr Arg Thr Asp Gly Thr Thr Leu Glu Asn Leu Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Gln Tyr Arg Ser Ser Arg Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Phe Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Gln Glu Gln Lys Glu Phe Leu 
    290                 295                 300                 


Val Asn Tyr Ala Lys Glu Thr Ser Val Leu Gly Ala Gly Lys Ile Leu 
305                 310                 315                 320 


Gln Gln Ile Ala Lys Leu Ala Asp Cys Lys Val Glu Asp Ile Arg Gly 
                325                 330                 335     


Tyr Arg Leu Asp Asn Lys Asp Lys Pro Glu Leu His Ile Phe Glu Thr 
            340                 345                 350         


Tyr Arg Ala Met Lys Gly Leu Val Pro Leu Val Asp Ile Gly Val Leu 
        355                 360                 365             


Ser Arg Glu Gln Leu Asp Ile Leu Ala Asp Ile Leu Thr Leu Asn Thr 
    370                 375                 380                 


Asp Phe Glu Gly Ile Arg Glu Ala Leu Lys Lys Gln Leu Pro Asn Val 
385                 390                 395                 400 


Phe Asp Glu Lys Gln Val Lys Gly Leu Ala Ser Phe Arg Lys Ser Lys 
                405                 410                 415     


Ser Gln Leu Phe Ala Lys Gly Trp His Asn Leu Ser Gln Lys Ile Met 
            420                 425                 430         


Leu Glu Val Ile Pro Glu Leu Tyr Ala Thr Ser Asp Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Phe Glu Lys Ser Ser Val Ala Glu Tyr 
    450                 455                 460                 


Pro Ser Ser Ile Asn Val Asp Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Ile Arg Gln Thr Ile Lys Ile Ile Asn Ala Ser 
                485                 490                 495     


Ile Lys Lys Trp Gly Glu Phe Asp Gln Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Arg Asn Glu Asp Glu Glu Lys Lys Arg Ile Ala Asp Gly Gln Lys 
        515                 520                 525             


Ala Asn Ala Lys Glu Lys Ala Asp Ser Ile Leu Arg Ala Ala Glu Leu 
    530                 535                 540                 


Tyr Cys Ala Gly Lys Val Leu Pro Asp Tyr Val Tyr Asn Gly His Asn 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Ile Tyr Thr Gly Gln Pro Ile Ser Ile His Asp Leu Ile His Asn Gln 
            580                 585                 590         


Asn Gln Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Ala Gln Arg Thr Pro Tyr Asn Tyr Leu Lys Ser Ala Thr Ser Ala 
625                 630                 635                 640 


Trp Ser Tyr Arg Glu Phe Lys Asp Tyr Val Thr Lys Arg Lys Gly Ile 
                645                 650                 655     


Gly Lys Lys Lys Cys Glu Tyr Leu Thr Phe Glu Glu Asp Ile Asn Gly 
            660                 665                 670         


Phe Glu Val Arg Ser Lys Phe Ile Gln Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Lys Val Ile Leu Asn Ala Leu Gln Asp Tyr Phe Lys Ile 
    690                 695                 700                 


Ser Gly Ile Gln Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg His Lys Trp Gly Ile Glu Lys Thr Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg 
            740                 745                 750         


Leu Trp Lys Lys Gln Glu Ser Pro Leu Val Val Asp Tyr Gln Glu Gly 
        755                 760                 765             


Arg Gln Val Asp Leu Glu Thr Gly Glu Ile Leu Glu Leu Thr Asp Glu 
    770                 775                 780                 


Gln Tyr Lys Glu Leu Val Tyr Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Ser Ala Phe Asp Asn Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Val Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala 
            820                 825                 830         


Thr Arg Asn Ala Gln Leu Gly Lys Asp Lys Thr Glu Gly Ile Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Thr Gln Ala Gly Tyr Glu Ala Phe 
    850                 855                 860                 


Leu Lys Arg Tyr Thr Lys Asp Lys Thr Ser Phe Leu Met Tyr His Lys 
865                 870                 875                 880 


Asp Leu Asp Thr Trp Glu Lys Val Ile Glu Ile Ile Leu Arg Asp Tyr 
                885                 890                 895     


Arg Glu Tyr Asp Glu Lys Gly Lys Glu Ile Gly Asn Pro Phe Glu Arg 
            900                 905                 910         


Tyr Arg Arg Glu Asn Gly Tyr Val Lys Lys Tyr Ser Arg Lys Gly Asn 
        915                 920                 925             


Gly Thr Ala Ile Lys Ser Leu Lys Tyr Tyr Asp Asn Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Glu Asn Ser Arg Asn Ala Val Val Leu Gln 
945                 950                 955                 960 


Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn Lys Glu Thr Gly 
                965                 970                 975     


Lys Tyr Glu Phe Leu Gly Ile Lys Tyr Ser Asp Leu Ser Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Glu Tyr Gly Ile Ser  Gln Glu Lys Tyr Asp  Ser Ile Lys 
        995                 1000                 1005             


Ile Ala  Glu Gly Val Ala Lys  Lys Ser Ile Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Gln Asp Leu Leu Phe  Ile Lys Asp Ile Glu  Asn Asn Phe 
    1025                 1030                 1035             


Gly Lys  Leu Leu Arg Phe Thr  Ser Lys Asn Asp Thr  Ser Lys His 
    1040                 1045                 1050             


Tyr Val  Glu Leu Lys Pro Tyr  Asp Lys Asn Lys Phe  Gly Thr Glu 
    1055                 1060                 1065             


Glu Pro  Leu Leu Pro Val Leu  Gly Asn Val Ala Lys  Ser Gly Gln 
    1070                 1075                 1080             


Cys Ile  Lys Gly Leu Asn Lys  Ser Asn Ile Ser Ile  Tyr Lys Val 
    1085                 1090                 1095             


Arg Thr  Asp Ile Leu Gly Tyr  Arg His Phe Ile Lys  Gln Glu Gly 
    1100                 1105                 1110             


Glu His  Pro Gln Leu Lys Phe  Lys Lys 
    1115                 1120         


<210>  685
<211>  1122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  685

Met Ser Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Val Ile Asp Ala Gln Thr Gly Glu Ile Ile His Ala Ser 
            20                  25                  30          


Ser Arg Ile Phe Pro Ser Ala Asn Ala Ala Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Thr Phe Arg Gly Ser Arg Arg Leu Ile Arg Arg Lys Lys His Arg Ile 
    50                  55                  60                  


Lys Arg Leu Asp Asp Leu Phe Asn Asp Phe His Ile Asn Leu Asp Gly 
65                  70                  75                  80  


Glu Met Ser Thr Asp Asn Pro Tyr Val Leu Arg Val Lys Gly Leu Ser 
                85                  90                  95      


Gln Lys Leu Thr Val Glu Glu Leu Tyr Ile Ser Ile Lys Asn Ile Met 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Ser Asp Asn Glu 
        115                 120                 125             


Ala Gly Arg Ser Asp Tyr Ala Lys Ala Ile Glu Arg Asn Arg Gln Leu 
    130                 135                 140                 


Leu Thr Ser Lys Thr Pro Gly Glu Ile Gln Leu Glu Arg Leu Glu Lys 
145                 150                 155                 160 


Tyr Gly Gln Leu Arg Gly Asn Phe Thr Ile Ile Asp Glu Glu Gly Gln 
                165                 170                 175     


Ser Gln Gln Ile Ile Asn Val Phe Ser Thr Ser Asp Tyr Val Lys Glu 
            180                 185                 190         


Val Glu Lys Ile Leu Asp Cys Gln Lys Met Tyr His Lys Phe Ile Ser 
        195                 200                 205             


Asp Glu Phe Cys Asp Lys Leu Ile Glu Leu Leu Arg Glu Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr Val Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Ile 
225                 230                 235                 240 


Tyr Arg Thr Asp Gly Thr Thr Leu Glu Asn Leu Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Gln Tyr Arg Ser Ser Arg Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Phe Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Gln Glu Gln Lys Glu Phe Leu 
    290                 295                 300                 


Val Asn Tyr Ala Lys Glu Thr Ser Val Leu Gly Ala Gly Lys Ile Leu 
305                 310                 315                 320 


Gln Gln Ile Ala Lys Leu Ala Asp Cys Lys Val Glu Asp Ile Arg Gly 
                325                 330                 335     


Tyr Arg Leu Asp Asn Lys Asp Lys Pro Glu Leu His Thr Phe Glu Thr 
            340                 345                 350         


Tyr Arg Ala Met Lys Gly Leu Val Pro Leu Val Asp Ile Gly Val Leu 
        355                 360                 365             


Ser Arg Glu Gln Leu Asp Ile Leu Ala Asp Ile Leu Thr Leu Asn Thr 
    370                 375                 380                 


Asp Phe Glu Gly Ile Arg Glu Ala Leu Lys Lys Gln Leu Pro Asn Val 
385                 390                 395                 400 


Phe Asp Glu Lys Gln Val Lys Gly Leu Ala Ser Phe Arg Lys Ser Lys 
                405                 410                 415     


Ser Gln Leu Phe Ala Lys Gly Trp His Asn Leu Ser Gln Lys Ile Met 
            420                 425                 430         


Leu Glu Val Ile Pro Glu Leu Tyr Ala Thr Ser Asp Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Phe Glu Lys Ser Ser Val Ala Glu Tyr 
    450                 455                 460                 


Pro Ser Ser Ile Asn Val Asp Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Ile Arg Gln Thr Ile Lys Ile Ile Asn Ala Ser 
                485                 490                 495     


Ile Lys Lys Trp Gly Glu Phe Asp Gln Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Arg Asn Glu Asp Glu Glu Lys Lys Arg Ile Ala Asp Gly Gln Lys 
        515                 520                 525             


Ala Asn Ala Lys Glu Lys Ala Asp Ser Ile Leu Arg Ala Ala Glu Leu 
    530                 535                 540                 


Tyr Cys Ala Gly Lys Val Leu Pro Asp Tyr Val Tyr Asn Gly His Asn 
545                 550                 555                 560 


Gln Leu Ala Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Ile Tyr Thr Gly Gln Pro Ile Ser Ile His Asp Leu Ile His Asn Gln 
            580                 585                 590         


Asn Gln Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Thr Phe Asp 
        595                 600                 605             


Asp Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Ala Gln Arg Thr Pro Tyr Asn Tyr Leu Lys Ser Ala Thr Ser Ala 
625                 630                 635                 640 


Trp Ser Tyr Arg Glu Phe Lys Asp Tyr Val Thr Lys Arg Lys Gly Ile 
                645                 650                 655     


Gly Lys Lys Lys Cys Glu Tyr Leu Thr Phe Glu Glu Asp Ile Asn Gly 
            660                 665                 670         


Phe Glu Val Arg Ser Lys Phe Ile Gln Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Lys Val Ile Leu Asn Ala Leu Gln Asp Tyr Phe Lys Ile 
    690                 695                 700                 


Ser Gly Ile Gln Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg His Lys Trp Gly Ile Glu Lys Thr Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg 
            740                 745                 750         


Leu Trp Lys Lys Gln Glu Ser Pro Leu Val Val Asp Tyr Gln Glu Gly 
        755                 760                 765             


Arg Gln Val Asp Leu Glu Thr Gly Glu Ile Leu Glu Leu Thr Asp Glu 
    770                 775                 780                 


Gln Tyr Lys Glu Leu Val Tyr Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Ser Ala Phe Asp Asn Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Val Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala 
            820                 825                 830         


Thr Arg Asn Ala Gln Leu Gly Lys Asp Lys Thr Glu Gly Ile Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Thr Gln Ala Gly Tyr Glu Ala Phe 
    850                 855                 860                 


Leu Lys Arg Tyr Thr Lys Asp Lys Thr Ser Phe Leu Met Tyr His Lys 
865                 870                 875                 880 


Asp Leu Asp Thr Trp Glu Lys Val Ile Glu Ile Ile Leu Arg Asp Tyr 
                885                 890                 895     


Arg Glu Tyr Asp Glu Lys Gly Lys Glu Ile Gly Asn Pro Phe Glu Arg 
            900                 905                 910         


Tyr Arg Arg Glu Asn Gly Tyr Val Lys Lys Tyr Ser Arg Lys Gly Asn 
        915                 920                 925             


Gly Thr Ala Ile Lys Ser Leu Lys Tyr Tyr Asp Asn Lys Leu Gly Asn 
    930                 935                 940                 


His Ile Asp Ile Thr Pro Glu Asn Ser Arg Asn Ala Val Val Leu Gln 
945                 950                 955                 960 


Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn Lys Glu Thr Gly 
                965                 970                 975     


Lys Tyr Glu Phe Leu Gly Ile Lys Tyr Ser Asp Leu Ser Phe Glu Lys 
            980                 985                 990         


Gly Thr Gly Glu Tyr Gly Ile Ser  Gln Glu Lys Tyr Asp  Ser Ile Lys 
        995                 1000                 1005             


Ile Ala  Glu Gly Val Ala Lys  Lys Ser Ile Phe Lys  Phe Thr Leu 
    1010                 1015                 1020             


Tyr Lys  Gln Asp Leu Leu Phe  Ile Lys Asp Ile Glu  Asn Asn Phe 
    1025                 1030                 1035             


Gly Lys  Leu Leu Arg Phe Thr  Ser Lys Asn Asp Thr  Ser Lys His 
    1040                 1045                 1050             


Tyr Val  Glu Leu Lys Pro Tyr  Asp Lys Asn Lys Phe  Gly Thr Glu 
    1055                 1060                 1065             


Glu Pro  Leu Leu Pro Val Leu  Gly Asn Val Ala Lys  Ser Gly Gln 
    1070                 1075                 1080             


Cys Ile  Lys Gly Leu Asn Lys  Ser Asn Ile Ser Ile  Tyr Lys Val 
    1085                 1090                 1095             


Arg Thr  Asp Ile Leu Gly Tyr  Arg His Phe Ile Lys  Gln Glu Gly 
    1100                 1105                 1110             


Glu His  Pro Gln Leu Lys Phe  Lys Lys 
    1115                 1120         


<210>  686
<211>  1123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  686

Met Ala Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Asp Asp Val Gln Asn Lys Ile Leu Gly Val Gly Ser Arg 
            20                  25                  30          


Ile Phe Pro Met Gly Val Glu Asn Leu Gly Asp Gly Asp Gly Glu Ile 
        35                  40                  45              


Ser Lys Asn Ala Ser Arg Thr Gly Thr Arg Gly Val Arg Arg Gln Phe 
    50                  55                  60                  


Phe Arg Arg Arg Leu Arg Lys Lys Val Leu Leu Lys Ala Leu Ser Glu 
65                  70                  75                  80  


Asn Asn Met Cys Pro Met Ile Thr Ser Asp Phe Asp Asn Trp Lys Lys 
                85                  90                  95      


Thr Lys Gln Phe Pro Ser Glu Lys Leu Ala Ser Trp Phe Ala Leu Asn 
            100                 105                 110         


Pro Tyr Glu Leu Arg Asn Lys Ala Leu Ser Glu Lys Leu Thr Leu Glu 
        115                 120                 125             


Glu Ile Gly Arg Ile Phe Tyr His Leu Ile Gln Arg Arg Gly Phe Leu 
    130                 135                 140                 


Ser Asn Ser Arg Lys Gly Gly Thr Asp Asp Gly Ala Ile Phe Lys Gly 
145                 150                 155                 160 


Asn Pro Lys Glu Gly Lys Ile Gly Ile Thr Asp Thr Gln Glu Ser Ile 
                165                 170                 175     


Gln Asp Lys Thr Leu Gly Ser Tyr Leu Phe Glu Ile Tyr Pro Lys Glu 
            180                 185                 190         


Asn Gln Pro Phe Gln Asn Gly Leu Glu Arg Ile Arg Asn Arg Tyr Thr 
        195                 200                 205             


Val Arg Lys Met Tyr Val Asp Glu Phe Glu Met Ile Trp Glu Lys Gln 
    210                 215                 220                 


Ala Gln Tyr Tyr Ser Asn Leu Asn Glu Asp Leu Lys Thr Leu Phe Gly 
225                 230                 235                 240 


Gly Arg Lys Leu Asp Gly Tyr Lys Glu Asp Gly Ile Leu Phe His Gln 
                245                 250                 255     


Arg Pro Leu Arg Ser Gln Lys His Leu Val Gly Asn Cys Val Phe Glu 
            260                 265                 270         


Pro Asn Lys Thr Lys Cys Pro Ile Ser Ala Ile Pro Phe Glu Gln Phe 
        275                 280                 285             


Arg Val Trp Gln Trp Val Asn Thr Val Glu Tyr Asn Gly Lys Lys Ile 
    290                 295                 300                 


Ser Gln Val Glu Lys Glu Lys Ile Val Glu Phe Leu Tyr Ala Asn Asp 
305                 310                 315                 320 


Lys Thr Asp Phe Lys Lys Ile Arg Lys Ala Ile Gly Lys Glu Ser Thr 
                325                 330                 335     


Glu Phe Lys Phe Asn Tyr Lys Asp Asp Asp Lys Ile Val Gly Thr Tyr 
            340                 345                 350         


Thr Val Ser His Leu Ser Asn Lys Lys Tyr Phe Gly Lys Lys Trp Phe 
        355                 360                 365             


Glu Phe Ser Glu Lys Glu Gln Glu Asp Ile Trp His Ile Leu Tyr Phe 
    370                 375                 380                 


Phe Asp Ser Lys Ser Asn Leu Lys Glu Tyr Ala Ile Lys Asn Trp Asn 
385                 390                 395                 400 


Phe Thr Glu Glu Gln Ala Glu Ser Ile Ala Lys Phe Asn Val Lys Asp 
                405                 410                 415     


Gly Tyr Ser Ser Leu Ser Arg Lys Ala Ile Ser Asn Ile Leu Pro Phe 
            420                 425                 430         


Leu Lys Leu Gly Phe Thr Tyr Asp Val Ala Val Val Leu Gly Gly Ile 
        435                 440                 445             


Lys Asn Val Phe Gly His Glu Trp Glu Lys Leu Ser Asp Glu Lys Arg 
    450                 455                 460                 


Asn Phe Leu Ile Asp Asn Val Glu Gly Ile Val Arg Ser Lys Thr Lys 
465                 470                 475                 480 


Gly Gly Phe Ile Asp Val Ile Lys Asp Ile Leu Arg Asn Asp Tyr Asn 
                485                 490                 495     


Ile Ser Asp Asn Gln Leu Arg Lys Leu Tyr His His Ser Ala Thr Ile 
            500                 505                 510         


Tyr Val Ala Glu Leu Leu Glu Lys Leu Pro Val Gly Lys Glu Ala Asp 
        515                 520                 525             


Lys Glu Ile Gln Ser Ile Arg Asn Pro Ile Val Ile Thr Ala Leu Phe 
    530                 535                 540                 


Glu Leu Arg Lys Leu Val Asn Glu Leu Ile Asp Asp Phe Gly Ser Ile 
545                 550                 555                 560 


Asp Glu Ile Lys Val Glu Met Ala Arg Asp Leu Lys Ile Ser Lys Ser 
                565                 570                 575     


Gln Arg Asn Lys Ile Arg Arg Glu Gln Lys Arg Leu Glu Arg Glu Asn 
            580                 585                 590         


Asp Arg Val Lys Ala Arg Leu Leu Glu Glu Gly Gln Arg Ile Thr His 
        595                 600                 605             


Asp Asn Ile Leu Leu Tyr Lys Leu Trp Glu Glu Cys Lys Lys Thr Cys 
    610                 615                 620                 


Pro Tyr Thr Gly Asn Pro Ile Pro Leu Tyr Lys Leu Phe Thr Gly Glu 
625                 630                 635                 640 


Ile Gln Ile Glu His Ile His Pro Trp Ser Arg Ser Leu Asn Asp Ser 
                645                 650                 655     


Phe Ala Asn Lys Thr Leu Cys Tyr Ala Asp Glu Asn Arg Lys Lys Gly 
            660                 665                 670         


Asp Lys Thr Pro Tyr Glu Phe Tyr Gly Asn Asp Glu Ser Asn Trp Ser 
        675                 680                 685             


Ala Ile Lys Glu Arg Ala Leu Lys Leu Phe Ser Asp Thr Lys Glu Tyr 
    690                 695                 700                 


Pro Asn Ala Tyr Gln Lys Phe Lys Arg Phe Val Gln His Lys Phe Asp 
705                 710                 715                 720 


Asp Asp Phe Ser Ser Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser Lys 
                725                 730                 735     


Glu Ala Lys Asn Tyr Leu Ser Lys Ile Cys Lys Lys Val Ala Val Ser 
            740                 745                 750         


Pro Gly Gln Ala Thr Ser Asn Leu Arg Gln Lys Trp Gly Met Asn Asn 
        755                 760                 765             


Ile Leu Asn Asp Glu Asn Glu Lys Thr Arg Glu Asp His Arg His His 
    770                 775                 780                 


Ala Ile Asp Ala Leu Val Met Ala Cys Thr Lys Ile Ser Tyr Val Gln 
785                 790                 795                 800 


Glu Leu Ser Lys Trp Asn Arg Tyr Asn Arg Asn Ala Glu Leu Lys Lys 
                805                 810                 815     


Phe Pro Leu Pro Trp Glu Thr Phe Trp Ser Asp Ala Asn Arg Ala Val 
            820                 825                 830         


Asp Lys Ile Leu Ile Ser His Lys Lys Val Ser Asn Asp Ile Thr Gly 
        835                 840                 845             


Arg Thr His Ile Ser Glu Lys Asn Gly Lys Lys Tyr Lys Asn Val Gly 
    850                 855                 860                 


Val Ala Ala Arg Gly Gln Leu His Lys Glu Thr Val Tyr Gly Lys Arg 
865                 870                 875                 880 


Thr Phe Asn Gly Glu Glu Ala Phe His Val Arg Lys Thr Ile Asp Ser 
                885                 890                 895     


Leu Thr Thr Glu Lys Gln Leu Glu Lys Val Val Asp Glu Ser Ile Lys 
            900                 905                 910         


Leu Leu Ile Lys Lys Arg Val Gln Glu Leu Gly Gly Phe Val Lys Gly 
        915                 920                 925             


Ala Ile Pro Ala Asn Thr Phe Phe Val Val Asp Glu Asn Gly Ile Lys 
    930                 935                 940                 


Gln Pro Gln Ile Phe Leu Pro Asn Lys Asn Gly Ala Pro Val Pro Val 
945                 950                 955                 960 


Leu Lys Val Arg Met Lys Glu Asn Ile Gly Gly Ala Glu Lys Leu Lys 
                965                 970                 975     


Glu Asn Val Asn Gln Trp Val Asn Pro Arg Asn Asn His His Val Leu 
            980                 985                 990         


Ile Tyr Lys Asp Glu Lys Gly Asn  Leu Lys Glu Glu Val  Val Thr Phe 
        995                 1000                 1005             


Trp Thr  Val Val Glu Arg Lys  Arg Thr Gly Gln Ser  Ala Tyr Gln 
    1010                 1015                 1020             


Leu Pro  Phe Asp Gly Lys Glu  Ile Val Thr Thr Leu  His Ile Asn 
    1025                 1030                 1035             


Asp Met  Phe Leu Leu Gly Leu  Asn Glu Glu Glu Ile  Asn Trp Glu 
    1040                 1045                 1050             


Asn Pro  Asp Tyr Glu Val Leu  Lys Glu His Leu Tyr  Arg Val Gln 
    1055                 1060                 1065             


Lys Phe  Thr Ser Gly Asp Tyr  Tyr Phe Arg Ile Ala  Lys Thr Ser 
    1070                 1075                 1080             


Ser Ile  Gln Asn Ala Ile Glu  Lys Gln Gln Ile Asn  Ser Phe Gly 
    1085                 1090                 1095             


Phe Gly  Lys Asn Gly Trp Ser  Thr His Asn Pro Ile  Lys Val Lys 
    1100                 1105                 1110             


Ile Ser  Val Ser Gly Lys Ile  Ser Lys Val 
    1115                 1120             


<210>  687
<211>  1123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  687

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Asp Thr Gly Glu Ile Ile His Thr Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Asn 
        35                  40                  45              


Ser Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Lys His Arg Gly Ala 
    50                  55                  60                  


Arg Leu His Asp Leu Phe Glu Asp Tyr Asp Leu Leu Ile Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Gln Gly Leu 
                85                  90                  95      


Asp Asn Pro Leu Thr Thr Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asn Gly 
        115                 120                 125             


Gly Ala Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Glu Glu Lys Gly Asp 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Arg Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Gln Ile Thr 
        195                 200                 205             


Asp Lys Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Glu Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asn Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Thr Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Ser Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Pro Val Gly Glu Phe Ser 
        355                 360                 365             


Arg Glu Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr Lys Leu Met Asp Val Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Ile Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Ala Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Phe Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Val Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn His Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Ser Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Asp Ser Lys Leu Leu Gly 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Thr Leu Gln Asp Phe Tyr Lys Ala His 
    690                 695                 700                 


Lys Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Ala Asn Pro Leu Ile Ser Tyr Lys Glu Asp Gln Phe 
        755                 760                 765             


Val Asp Ser Glu Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Lys Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Ala His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Ser Ser Lys Lys Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Val Lys Leu Asp Lys Asp Lys Ser Glu Glu Thr Tyr Val Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Gln Ala Gly Tyr Asp Ala Phe Ile Lys 
    850                 855                 860                 


Ile Tyr Asn Lys Asp Lys Ser Lys Phe Leu Met Tyr His Lys Asp Pro 
865                 870                 875                 880 


Gln Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Arg Thr Tyr Pro Ser 
                885                 890                 895     


Lys Glu Phe Asn Glu Lys Asn Lys Glu Val Pro Cys Asn Pro Phe Glu 
            900                 905                 910         


Lys Tyr Arg Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly 
        915                 920                 925             


Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Lys Asn Ile Pro 
    930                 935                 940                 


Glu Ser Phe Ile Asn Ile Thr Pro Ser Asn Ser Lys Asn Lys Val Ile 
945                 950                 955                 960 


Leu Arg Gln Leu Asn Pro Trp Arg Thr Asp Val Tyr Phe Asn His Gln 
                965                 970                 975     


Thr Glu Lys Tyr Glu Leu Leu Gly Leu Lys Tyr Ala Asp Leu Gln Phe 
            980                 985                 990         


Glu Lys Lys Thr Gly Thr Tyr Ser  Ile Thr Ile Asp Lys  Tyr Asn Glu 
        995                 1000                 1005             


Ile Lys  Glu Lys Glu Gly Val  Gly Glu Gln Ser Glu  Phe Lys Phe 
    1010                 1015                 1020             


Thr Leu  Tyr Lys Asn Asp Leu  Leu Leu Ile Lys Asp  Thr Lys Asn 
    1025                 1030                 1035             


Asn Glu  Lys Gln Ile Phe Arg  Phe Leu Ser Arg Thr  Met Pro Asn 
    1040                 1045                 1050             


Val Lys  His Tyr Val Glu Leu  Lys Pro Tyr Asp Lys  Ala Lys Phe 
    1055                 1060                 1065             


Asp Gly  Asn Gln Pro Leu Ile  Thr Ile Phe Gly Asn  Val Ala Lys 
    1070                 1075                 1080             


Gly Gly  Gln Cys Leu Lys Gly  Leu Asn Lys Ser Asn  Ile Ser Ile 
    1085                 1090                 1095             


Phe Lys  Val Lys Thr Asp Val  Leu Gly Asn Lys His  Phe Ile Thr 
    1100                 1105                 1110             


Lys Glu  Gly Glu Lys Pro Lys  Leu Lys Phe 
    1115                 1120             


<210>  688
<211>  1125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  688

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Glu Thr Gly Glu Ile Ile His Val Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Ser Asn Val Glu Arg Arg Gly 
        35                  40                  45              


Phe Arg Gln Gly Arg Arg Leu Gly Arg Arg Lys Lys His Arg Ser Ala 
    50                  55                  60                  


Arg Leu Asn Asp Leu Phe Glu Glu Phe Gly Phe Ile Thr Asp Phe Ser 
65                  70                  75                  80  


Ala Val Pro Leu Asn Leu Asn Pro Tyr Ala Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Glu Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Ile Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Glu Thr Ala Ser Asn Glu Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Asp Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Asn His Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Arg Gln Gln Glu Phe Asn Val Arg Ile Ser 
        195                 200                 205             


Asp Glu Phe Ile Glu Ala Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Tyr Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Arg Pro Glu Gln Lys Arg Gln Ile 
    290                 295                 300                 


Val Glu Tyr Ala Arg Thr Ala Lys Thr Leu Gly Thr Pro Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Val Asp Gly Ser Ile Asp Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Asp Lys Pro Glu Met His Thr Phe Asp Ala 
            340                 345                 350         


Tyr Arg Lys Met Arg Thr Leu Glu Leu Val Asp Val Asp Ile Leu Ser 
        355                 360                 365             


Arg Glu Thr Leu Asp Asp Leu Ala Tyr Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Ser Glu Gly Ile Leu Glu Ala Leu Asn Ser Lys Met Pro Gly Thr Phe 
385                 390                 395                 400 


Thr Lys Glu Gln Ile Asp Glu Leu Ile Gln Phe Arg Lys Lys Asn Ser 
                405                 410                 415     


Ala Val Phe Gly Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Arg Ser Arg Glu Ile Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Leu Thr Asp Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Leu Ala Thr 
                485                 490                 495     


Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Ser Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Asn Val Gln Lys Ala 
        515                 520                 525             


Asn Glu Asp Glu Lys Lys Ala Ala Met Glu Lys Ala Ala Asp Leu Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Ser Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Asn Ile Ser Ile His Asp Leu Ile His Asn Pro His 
            580                 585                 590         


Gln Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Gly Leu Ala Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Ile Glu Phe Lys Gln Tyr Val Arg Asn Ser Lys Ser Leu Ser 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Thr Leu Gln Glu Phe Tyr Lys Thr Asn 
    690                 695                 700                 


Asp Phe Asp Thr Lys Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Lys Ile Glu Lys Ser Arg Asp Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Pro Leu Ile Ser Tyr Arg Glu Gly Gln Leu 
        755                 760                 765             


Val Asp Pro Glu Thr Gly Glu Ile Leu Ser Leu Thr Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Leu Arg Pro Pro Tyr Asp Tyr Phe Val Asp Thr Leu 
785                 790                 795                 800 


Lys Ser Lys Ser Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Gly Thr Arg 
            820                 825                 830         


Lys Ala Gln Leu Gly Lys Asp Lys Gln Glu Glu Thr Tyr Val Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Gln Lys Gly Tyr Glu Asp Phe Ile Lys 
    850                 855                 860                 


Arg Tyr Lys Lys Asp Thr Thr Gln Phe Leu Met Tyr His Lys Asp Pro 
865                 870                 875                 880 


Gln Thr Phe Ala Lys Val Ile Glu Glu Ile Leu Lys Thr Tyr Pro Asp 
                885                 890                 895     


Lys Glu Leu Asn Glu Lys Gly Lys Glu Ile Pro Cys Asn Pro Phe Glu 
            900                 905                 910         


Lys Tyr Arg Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly 
        915                 920                 925             


Lys Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Asn Lys Leu Gly 
    930                 935                 940                 


Asn His Ile Asp Ile Thr Pro Val Asn Ser Gln Asn Gln Val Val Leu 
945                 950                 955                 960 


Gln Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn Pro Gln Thr 
                965                 970                 975     


Ser Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu Arg Phe Glu 
            980                 985                 990         


Lys Gly Ser Gly Ser Tyr Gly Ile  Ser Pro Glu Lys Tyr  Asn Lys Val 
        995                 1000                 1005             


Lys Ala  Lys Glu Gly Val Asp  Glu Asp Ser Glu Phe  Lys Phe Thr 
    1010                 1015                 1020             


Leu Tyr  Lys Asn Asp Leu Ile  Leu Ile Lys Asp Thr  Glu Thr Gly 
    1025                 1030                 1035             


Glu Gln  Gln Leu Phe Arg Tyr  Gly Ser Arg Asn Asp  Thr Ser Lys 
    1040                 1045                 1050             


His Tyr  Val Glu Leu Lys Pro  Tyr Glu Lys Ala Lys  Phe Glu Gly 
    1055                 1060                 1065             


Asn Gln  Gln Leu Met Asn Leu  Leu Gly Thr Val Ala  Lys Gly Gly 
    1070                 1075                 1080             


Gln Cys  Leu Lys Gly Ile Asn  Lys Pro Asn Leu Ser  Ile Tyr Lys 
    1085                 1090                 1095             


Val Lys  Thr Asp Val Leu Gly  Asn Lys Tyr Phe Ile  Lys Lys Glu 
    1100                 1105                 1110             


Gly Asp  Gln Pro Gln Leu Asn  Phe Lys Lys Lys Ile  
    1115                 1120                 1125 


<210>  689
<211>  1125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  689

Met Thr Ser Lys Gln Glu Asn Leu Gly Ala Leu Thr Phe Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Val Leu Gly Glu Asn Arg Ile 
            20                  25                  30          


Ile Asp Leu Gly Val Arg Cys Phe Asp Lys Ala Glu Thr Ala Lys Glu 
        35                  40                  45              


Gly Glu Ser Leu Asn Leu Ala Arg Arg Met Ala Arg Leu Leu Arg Arg 
    50                  55                  60                  


Arg Leu Arg Arg Arg Ala Trp Arg Leu Thr Lys Leu Ala Arg Leu Leu 
65                  70                  75                  80  


Lys Arg Glu Gly Leu Ile Ala Asp Ala Asn Leu Phe Lys Asn Lys Ile 
                85                  90                  95      


Glu Lys Asn Thr Pro Val Pro Lys Ser Ser Trp Gln Leu Arg Val Asp 
            100                 105                 110         


Ala Leu Asp Arg Lys Leu Asn Ala Glu Glu Trp Ala Arg Val Ile Tyr 
        115                 120                 125             


His Leu Cys Lys His Arg Gly Phe His Trp Ile Ser Arg Ala Glu Glu 
    130                 135                 140                 


Lys Ala Ala Glu Ser Asp Ser Lys Gly Glu Ser Gly Lys Val Lys Gln 
145                 150                 155                 160 


Gly Leu Ala Gly Thr Lys Lys Leu Met Gln Glu Lys Asn Tyr Arg Thr 
                165                 170                 175     


Ala Ala Glu Met Val Leu Ala Glu Phe Pro Glu Ala Gln Arg Asn Lys 
            180                 185                 190         


Gln Gly Asp Tyr Ser Lys Ala Leu Ser Arg Ile Leu Leu Gly Asp Glu 
        195                 200                 205             


Leu Lys Thr Leu Phe Glu His Gln Arg Lys Leu Asp Ser Pro Phe Ala 
    210                 215                 220                 


Thr Glu Lys Leu Glu Ala Ala Ile Leu Gly Asn Gly Asp Arg Lys Ser 
225                 230                 235                 240 


Gly Leu Phe Trp Ala Gln Asn Pro Ala Leu Ala Gly Ser Asp Leu Leu 
                245                 250                 255     


Lys Met Leu Gly Tyr Cys Thr Phe Glu Lys Thr Glu Tyr Arg Ala Pro 
            260                 265                 270         


Lys Ala Ser Phe Thr Ala Glu Arg His Val Trp Leu Thr Arg Leu Asn 
        275                 280                 285             


Asn Leu Arg Ile Val Val Asp Gly Thr Thr Arg Pro Leu Asn Glu Ala 
    290                 295                 300                 


Glu Arg Arg Ile Ala Leu Leu Met Pro Tyr Gln Gln Ala Gly Asp Phe 
305                 310                 315                 320 


Thr Tyr Lys Gln Leu Arg Thr Ala Leu Thr Lys Ala Gly Phe Leu Pro 
                325                 330                 335     


Glu Ser Phe Arg Tyr Thr Gly Leu Ser Tyr Ala Ser Gly Gln Lys Ala 
            340                 345                 350         


Gly Glu Lys Thr Lys Asp Pro Glu Ser Ala Ala Leu Val Lys Phe Pro 
        355                 360                 365             


Ala Trp Gln Glu Leu Arg Lys Thr Leu Glu Lys Lys Glu Leu Ala Ser 
    370                 375                 380                 


Glu Trp Gln Lys Ile Ser Thr Asp Ala Leu Asp Gly Lys Pro Gln Leu 
385                 390                 395                 400 


Leu Asp Gln Ile Ala Trp Val Leu Ser Val Tyr Lys Asp Asp Asp Glu 
                405                 410                 415     


Val Lys Ala Glu Leu Ser Lys Leu Asn Leu Pro Asn Ala Glu Lys Met 
            420                 425                 430         


Ile Asp Ala Leu Leu Asp Ile Arg Phe Asp Lys Phe Ser Asn Leu Ser 
        435                 440                 445             


Leu Lys Ala Leu Cys Lys Ile Val Pro Leu Met Glu Gln Gly Gln Arg 
    450                 455                 460                 


Tyr Asp Glu Ala Ile Ala Asn Ile Pro Glu Tyr Gly His His Ser Gln 
465                 470                 475                 480 


Leu His Lys Val Gly Ala Gly Glu His Lys Tyr Leu Pro Pro Phe Tyr 
                485                 490                 495     


Lys Glu Arg Asp Lys Thr Gly Arg Met Val Phe Asn Asp Asp Met Asp 
            500                 505                 510         


Ile Pro Arg Asn Pro Val Val Leu Arg Ala Leu Asn Gln Ala Arg Lys 
        515                 520                 525             


Val Val Asn Ala Leu Ile Lys Lys Tyr Asp Ser Pro His Glu Val His 
    530                 535                 540                 


Ile Glu Met Ala Arg Asp Leu Ser Arg Pro Leu Asp Glu Arg Arg Glu 
545                 550                 555                 560 


Val Glu Lys Leu Gln Lys Glu Phe Arg Asp Asn Asn Glu Lys Gly Lys 
                565                 570                 575     


Ala Gln Phe Ala Gln Asp Phe Asn Ile Val Gly Ala Val Lys Gly Lys 
            580                 585                 590         


Glu Phe Glu Lys Tyr Gln Leu Tyr Arg Glu Gln Gln Gly Lys Cys Ala 
        595                 600                 605             


Tyr Ser Ile Glu Pro Met Asp Ile His Arg Leu Phe Glu Gln Gly Tyr 
    610                 615                 620                 


Val Glu Ile Asp His Ala Leu Pro Tyr Ser Arg Ser Phe Asp Asp Ser 
625                 630                 635                 640 


Lys Asn Asn Arg Val Leu Val Leu Ala Lys Glu Asn Arg Asp Lys Gly 
                645                 650                 655     


Asn Arg Thr Pro Phe Glu Tyr Leu Gly Gly Lys Asp Asn Ser Glu Pro 
            660                 665                 670         


Trp Arg Gln Phe Val Ala Phe Val Glu Gly Asn Lys Ala Tyr Arg Leu 
        675                 680                 685             


Ala Lys Arg Ser Arg Leu Leu Arg Lys Asp Phe Gly Asp Lys Glu Ala 
    690                 695                 700                 


Lys Glu Phe Arg Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ile Cys Lys 
705                 710                 715                 720 


Phe Phe Lys Asn Tyr Val Glu Gln Tyr Leu Gln Leu His Glu Asp Ser 
                725                 730                 735     


Glu Ala Lys Arg Cys Val Val Leu Ser Gly Gln Met Thr Ser Phe Leu 
            740                 745                 750         


Arg Ala Arg Trp Gly Leu Val Lys Val Arg Gly Asp Ser Asp Arg His 
        755                 760                 765             


His Ala Leu Asp Ala Ala Val Val Ala Ala Cys Ser His Gly Met Val 
    770                 775                 780                 


Lys Arg Leu Ser Asp Tyr Ser Arg Arg Lys Glu Leu Glu Gln Val Arg 
785                 790                 795                 800 


Glu Gly Phe Val Asp Val Asp Thr Gly Glu Ile Val Asn Pro Ala Met 
                805                 810                 815     


Phe Gln Gln Val Gln Glu His Phe Pro Asn Pro Trp Pro His Phe Arg 
            820                 825                 830         


Asp Glu Val Lys Leu Arg Leu Ser Met Asp Asp Pro Ala Leu Leu Arg 
        835                 840                 845             


Ser Glu Met Glu Lys Phe Gly Thr Tyr Thr Pro Glu Ala Leu Gln Ala 
    850                 855                 860                 


Leu His Pro Leu Phe Val Ser Arg Ala Pro Gln Arg Arg Asn Ser Gly 
865                 870                 875                 880 


Ala Ala His Lys Asp Thr Ile Tyr Ala Gln Pro Lys Arg Leu Val Ala 
                885                 890                 895     


Gln Gly Gly Val Thr Gln Lys Val Ala Leu Thr Ser Leu Thr Leu Lys 
            900                 905                 910         


Asp Leu Asp Lys Leu Ile Asp Pro His Arg Asn Asp Lys Leu Tyr Thr 
        915                 920                 925             


Ala Ile Arg Ala Arg Leu Glu Gln His Gly Gly Lys Gly Asp Lys Ala 
    930                 935                 940                 


Phe Pro Ser Ser Asn Pro Leu Arg Lys Pro Asp Ser Glu Gly Asn Pro 
945                 950                 955                 960 


Thr Gly Pro Ile Val Arg Gly Val Thr Met Val Ile Asp Lys Leu Ser 
                965                 970                 975     


Gly Ile Pro Ile Arg Gly Gly Ile Ala Lys Asn Asp Ser Met Val Arg 
            980                 985                 990         


Val Asp Val Phe Thr Lys Ala Asn  Lys Phe His Leu Val  Pro Val Tyr 
        995                 1000                 1005             


Val His  His Thr Val Ala Lys  Ala Leu Pro Asn Arg  Ala Val Val 
    1010                 1015                 1020             


Ala Phe  Lys Asp Glu Asp Glu  Trp Thr Leu Ile Asp  Asp Ser Phe 
    1025                 1030                 1035             


Asp Phe  Cys Phe Ser Leu His  Pro Asn Asp Leu Val  Gln Val Gln 
    1040                 1045                 1050             


Gln Lys  Gly Lys Ala Gly Met  Ile Gly Tyr Tyr Ser  Ser Cys His 
    1055                 1060                 1065             


Arg Gly  Thr Gly Asn Ile Asn  Leu Trp Ala His Asp  Arg Asn Leu 
    1070                 1075                 1080             


Gln Val  Gly Lys Asp Gly Met  Ile Glu Gly Ile Gly  Val Lys Thr 
    1085                 1090                 1095             


Ala Leu  Ser Leu Glu Lys Phe  Asn Val Asp Val Leu  Gly Asn Arg 
    1100                 1105                 1110             


Tyr Pro  Ala Pro Pro Glu Lys  Arg Arg Asp Leu Ala  
    1115                 1120                 1125 


<210>  690
<211>  1125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  690

Met Thr Asp Arg Ile Ser Leu Gly Leu Asp Ile Gly Val Ala Ser Val 
1               5                   10                  15      


Gly Phe Ser Val Leu Asp Ile Asp Lys Gly Lys Val Ile Glu Leu Gly 
            20                  25                  30          


Ala Arg Leu Phe Asn Ala Thr Val Ala Ala Gly Asn Gln Asp Arg Arg 
        35                  40                  45              


Asp Met Arg Gly Ser Arg Arg Leu Leu Asn Arg Asn Lys Gln Arg Arg 
    50                  55                  60                  


Lys Asp Val Ser Lys Leu Phe Gln Glu Tyr Gly Leu Leu Asp Asn Phe 
65                  70                  75                  80  


Asp Arg Asp Asn Phe His Thr Ala Phe Asn Asn Asn Trp Asn Pro Tyr 
                85                  90                  95      


Glu Leu Arg Val Lys Gly Leu Thr Lys Gln Leu Asn Lys Glu Glu Leu 
            100                 105                 110         


Ala Asp Ser Leu Tyr Gln Ile Ile Lys Arg Arg Gly Ile Ser Tyr Ala 
        115                 120                 125             


Leu Lys Asp Ala Asp Ala Asn Glu Asp Gly Thr Asp Tyr Ser Ser Ser 
    130                 135                 140                 


Leu Lys Ile Asn Ser Gln Glu Leu Ile Glu Lys Thr Pro Ala Gln Ile 
145                 150                 155                 160 


Gln Leu Gln Arg Leu Asn Asp Tyr Gly Lys Val Arg Gly Lys Val Ile 
                165                 170                 175     


Val Gly Asp Asp Ile Asp Asp Gln Lys Val Leu Leu Asn Val Phe Pro 
            180                 185                 190         


Thr Asn Ala Tyr Glu Lys Glu Ala Arg Gln Ile Ile Ala Thr Gln Gln 
        195                 200                 205             


Gln Phe Tyr Pro Glu Ile Leu Thr Gly Glu Phe Val Lys Glu Tyr Cys 
    210                 215                 220                 


Gln Ile Leu Thr Arg Lys Arg Asp Tyr Phe Val Gly Pro Gly Asn Glu 
225                 230                 235                 240 


Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Lys Thr Asp Gly Arg Thr Leu 
                245                 250                 255     


Asp Asn Leu Phe Glu Glu Leu Ile Gly His Asp Lys Ile Tyr Pro Asp 
            260                 265                 270         


Glu Leu Arg Ala Ser Ala Ala Ser Tyr Thr Ala Gln Leu Phe Asn Val 
        275                 280                 285             


Leu Asn Asp Leu Asn Asn Leu Arg Ile Leu Ser Tyr Glu Asp Gly Lys 
    290                 295                 300                 


Leu Thr Gln Ile Asp Lys Glu Lys Ile Ile Ala Glu Ile Lys Asn Asn 
305                 310                 315                 320 


Thr Thr Val Val Asn Met Leu Asn Val Ile Lys Lys Val Thr Gly Cys 
                325                 330                 335     


Gln Lys Asp Asp Ile Lys Gly Phe Arg Leu Asn Glu Lys Asp Lys Pro 
            340                 345                 350         


Glu Ile Ser Ser Met Pro Ile Tyr Arg Lys Ala His Lys Asp Phe Leu 
        355                 360                 365             


Lys Val Gly Ile Asp Ile Thr Asp Trp Pro Ile Asp Phe Ile Asp Arg 
    370                 375                 380                 


Leu Ser Phe Ile Leu Thr Leu Asn Thr Glu Asn Gly Glu Ile Arg Lys 
385                 390                 395                 400 


Gln Ile Asn Lys Arg Leu Val Pro Asp Phe Asp Phe Leu Asn Asp Asp 
                405                 410                 415     


Leu Val Gln Leu Ile Ile Asp Asn Lys Asp Ser Phe Asp Ile Lys Thr 
            420                 425                 430         


Asn Asn Lys Trp His Arg Phe Ser Leu Lys Thr Met Asn Lys Leu Ile 
        435                 440                 445             


Pro Thr Met Ile Glu Arg Pro Val Glu Gln Met Thr Leu Leu Thr Glu 
    450                 455                 460                 


Met Gly Leu Ile Lys Lys Asp Thr Lys Arg Phe Glu Gly Asn Lys Tyr 
465                 470                 475                 480 


Leu Pro Tyr Lys Glu Ile Ala Asn Asp Ile Phe Asn Pro Val Ala Ser 
                485                 490                 495     


Lys Ser Val Arg Glu Ala Leu Lys Ile Val Asn Ala Val Leu Lys Lys 
            500                 505                 510         


Tyr Gly His Ile Glu Tyr Ile Val Ile Glu Met Pro Arg Asp Lys Asn 
        515                 520                 525             


Leu Asp Glu Glu Arg Lys Gln Ile Ala Asp Phe Gln Lys Lys Asn Lys 
    530                 535                 540                 


Lys Ile Lys Asp Ala Ala Phe Asn Ala Phe Val Lys Ala Val Gly Ser 
545                 550                 555                 560 


Lys Lys Asn Val Lys Ile Ala Leu Ser Lys Asn Arg Lys Leu Gln Met 
                565                 570                 575     


Gly Ile Arg Leu Trp His Gln Gln Gln Gly Ile Asp Pro Tyr Asn Gly 
            580                 585                 590         


Glu Glu Ile Asn Ala Asn Asp Leu Ile Ser Asn Pro Asp Lys Phe Glu 
        595                 600                 605             


Ile Asp His Ile Ile Pro Gln Ser Ile Ser Phe Asp Asp Gly Ile Asn 
    610                 615                 620                 


Asn Lys Thr Leu Cys Phe Ala Ser Met Asn Gln Val Lys Gly Gln Lys 
625                 630                 635                 640 


Thr Pro Tyr Glu Phe Leu Asn Glu Gly His Gly Gln Gly Tyr Ala Lys 
                645                 650                 655     


Phe Lys Ala Ile Val Ser Lys Asn Lys Asn Phe Ser Lys Ala Lys Arg 
            660                 665                 670         


Asp Asn Tyr Leu Phe Glu Glu Asn Val Ser Asn Ile Glu Thr Arg Lys 
        675                 680                 685             


Arg Phe Leu Ser Arg Asn Leu Val Asp Thr Arg Tyr Ser Ser Arg Val 
    690                 695                 700                 


Val Leu Asn Ser Leu Gln Glu Phe Phe His Glu Lys Ser Asp Asp Thr 
705                 710                 715                 720 


Lys Val Thr Val Ile Arg Gly Lys Phe Thr Ser Asn Met Arg Lys His 
                725                 730                 735     


Trp His Ile Asp Lys Thr Arg Asp Thr Phe His His His Ala Ile Asp 
            740                 745                 750         


Ala Ser Ile Ile Ala Ala Thr Pro Phe Leu Arg Met Trp Lys Lys Gly 
        755                 760                 765             


Ser Thr Ile Phe Pro Thr Lys Ile Asn Glu Thr Ser Ile Asp Ile Glu 
    770                 775                 780                 


Thr Gly Glu Ile Leu Asp Asp Lys Asn Phe Asp Lys Val Met Tyr Glu 
785                 790                 795                 800 


Glu Pro Tyr Ser Gly Phe Ile Ser Glu Ile Met Asn Ala Asp Asp Arg 
                805                 810                 815     


Ile Lys Phe Ser His Gln Val Asp Lys Lys Met Asn Arg Lys Val Ser 
            820                 825                 830         


Asp Ala Thr Ile Tyr Ser Thr Arg Ile Gly Lys Leu Ser Lys Asp Lys 
        835                 840                 845             


Lys Asp Ala Glu Tyr Val Val Ala Lys Val Lys Asp Ile Tyr Ser Val 
    850                 855                 860                 


Asp Gly Tyr Glu Asn Phe Lys Lys Val Tyr Lys Lys Asp Lys Thr Lys 
865                 870                 875                 880 


Phe Leu Leu Tyr Lys Tyr Asp Pro Arg Thr Phe Ser Glu Leu Glu Lys 
                885                 890                 895     


Ile Val Ile Asp Tyr Pro Asp Lys Ile Glu Lys Val Gln Thr Asn Gly 
            900                 905                 910         


Lys Ile Lys Ala Val Asp Ile Ser Pro Phe Glu Leu Tyr Arg Arg Asp 
        915                 920                 925             


Asn Gly Met Val Arg Lys Tyr Ser Lys Lys Thr Gly Pro Ala Ile Lys 
    930                 935                 940                 


Gln Leu Lys Tyr Leu Asp Lys Lys Leu Gly Ser His Ile Asp Ile Thr 
945                 950                 955                 960 


Pro Lys Asn Ala Asn Asn Lys His Val Val Leu Gln Ser Leu Lys Pro 
                965                 970                 975     


Trp Arg Thr Asp Val Tyr Leu Asn His Glu Thr Gly Glu Tyr Glu Ile 
            980                 985                 990         


Met Gly Ile Lys Tyr Ser Asp Leu  Lys Phe Asn Lys Asn  Glu Gly Tyr 
        995                 1000                 1005             


Gly Ile  Lys Lys Asp Lys Tyr  Leu Lys Ile Lys Asn  Ile Glu Gly 
    1010                 1015                 1020             


Val Ser  Glu Asn Ser Glu Phe  Met Phe Ser Leu Tyr  Arg Lys Asp 
    1025                 1030                 1035             


Arg Ile  Lys Val Gln Asp Met  Glu Thr Asn Glu Ser  Val Glu Leu 
    1040                 1045                 1050             


Leu Phe  Trp Ser Arg Asn Phe  Ser Asn Lys Lys Tyr  Ala Glu Leu 
    1055                 1060                 1065             


Lys Pro  Ile Ser Gln Ile Asp  Asn Asp Lys Val Leu  Pro Ile Tyr 
    1070                 1075                 1080             


Gly Arg  Gly Arg Leu Ile Lys  Arg Leu Ile Pro Lys  Asn Cys Lys 
    1085                 1090                 1095             


Ile Trp  Lys Val Asn Thr Ser  Ile Leu Gly Asp Thr  Tyr Tyr Leu 
    1100                 1105                 1110             


Glu Lys  Glu Ser Asn Tyr Pro  Gln Lys Ile Leu Asp  
    1115                 1120                 1125 


<210>  691
<211>  1126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  691

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Glu Thr Gly Glu Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Ser Asn Val Glu Arg Arg Gly 
        35                  40                  45              


Phe Arg Gln Gly Arg Arg Leu Gly Arg Arg Lys Lys His Arg Ser Ala 
    50                  55                  60                  


Arg Leu Asn Asn Leu Phe Glu Glu Phe Gly Phe Ile Thr Asp Phe Ser 
65                  70                  75                  80  


Ala Ile Pro Leu Asn Leu Asn Pro Tyr Ala Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Glu Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Ile Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Glu Thr Ala Ser Asn Glu Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Asp Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Asn His Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Arg Gln Gln Glu Phe Asn Val Arg Ile Ser 
        195                 200                 205             


Asp Glu Phe Ile Glu Ala Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Tyr Arg Ala Ala Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Pro Glu Gln Lys Arg Gln Ile 
    290                 295                 300                 


Val Glu Tyr Ala Arg Thr Ala Lys Thr Leu Gly Thr Pro Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Leu Val Asp Gly Ser Ile Asp Asp Ile Lys Gly 
                325                 330                 335     


Tyr Arg Ile Asp Lys Ser Asp Lys Pro Glu Met Met His Thr Phe Asp 
            340                 345                 350         


Ala Tyr Arg Lys Met Arg Thr Leu Asp Leu Val Asn Ile Asp Ala Leu 
        355                 360                 365             


Ser Arg Glu Thr Leu Asp Asp Leu Ala His Ile Leu Thr Leu Asn Thr 
    370                 375                 380                 


Glu Ser Glu Gly Ile Leu Glu Ala Leu Asn Ser Lys Met Pro Ser Thr 
385                 390                 395                 400 


Phe Thr Lys Glu Gln Ile Asp Glu Leu Ile Gln Phe Arg Lys Lys Asn 
                405                 410                 415     


Ser Ala Val Phe Gly Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met 
            420                 425                 430         


Asn Glu Leu Ile Ser Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Leu Gly Lys Gln Arg Ser Arg Glu Ile Ser Lys Arg 
    450                 455                 460                 


Thr Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala 
                485                 490                 495     


Thr Lys Arg Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
            500                 505                 510         


Glu Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys 
        515                 520                 525             


Ala Asn Gln Asp Glu Lys Asn Ala Ser Met Glu Lys Ala Ala Phe Gln 
    530                 535                 540                 


Tyr Asn Gly Lys Lys Glu Leu Pro Asp Ser Ile Phe His Gly His Lys 
545                 550                 555                 560 


Glu Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Thr Gly Lys Asn Ile Ser Ile Arg Asp Leu Ile His Asn Pro 
            580                 585                 590         


His Gln Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Gly Leu Ala Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Phe Gln Ala Ile Asp Ser Met Asp Asp Ala 
625                 630                 635                 640 


Trp Ser Tyr Arg Glu Phe Lys Gln Tyr Val Arg Asn Ser Lys Ser Leu 
                645                 650                 655     


Ser Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
            660                 665                 670         


Ile Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ser Ser Arg Val Val Leu Asn Thr Leu Gln Glu Phe Tyr Lys Thr 
    690                 695                 700                 


Asn Asp Phe Asp Thr Lys Ile Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Glu Lys Ser Arg Asp Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg 
            740                 745                 750         


Leu Trp Lys Lys Gln Asn Asn Pro Leu Ile Ser Tyr Lys Glu Gly Gln 
        755                 760                 765             


Phe Val Asp Pro Glu Thr Gly Glu Ile Leu Ser Leu Thr Asp Asp Glu 
    770                 775                 780                 


Tyr Lys Glu Leu Val Phe Arg Pro Pro Tyr Asp Tyr Phe Val Asp Thr 
785                 790                 795                 800 


Leu Lys Ser Lys Ser Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val 
                805                 810                 815     


Asp Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Gly Thr 
            820                 825                 830         


Arg Lys Ala Gln Leu Gly Lys Asp Lys Gln Glu Glu Thr Tyr Val Leu 
        835                 840                 845             


Gly Lys Ile Lys Asp Ile Tyr Ser Gln Lys Gly Tyr Glu Asp Phe Ile 
    850                 855                 860                 


Lys Arg Tyr Asn Lys Asp Glu Thr Gln Phe Leu Ile Tyr His Lys Asp 
865                 870                 875                 880 


Pro Gln Thr Phe Glu Lys Val Ile Glu Glu Ile Leu Lys Thr Tyr Pro 
                885                 890                 895     


Asp Lys Glu Leu Asn Glu Lys Gly Lys Glu Ile Pro Cys Asn Pro Phe 
            900                 905                 910         


Glu Lys Tyr Arg Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys 
        915                 920                 925             


Gly Lys Gly Pro Glu Ile Lys Ile Leu Lys Tyr Tyr Asp Asn Lys Leu 
    930                 935                 940                 


Gly Asn His Ile Asp Ile Thr Pro Val Asn Ser Gln Asn Gln Val Val 
945                 950                 955                 960 


Leu Gln Ser Leu Lys Pro Trp Arg Thr Asp Val Tyr Phe Asn Pro Arg 
                965                 970                 975     


Thr Ser Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu Arg Phe 
            980                 985                 990         


Glu Lys Gly Ser Gly Ser Tyr Gly  Ile Ser Pro Glu Lys  Tyr Asn Lys 
        995                 1000                 1005             


Val Lys  Ala Lys Glu Gly Val  Asn Glu Asp Ser Glu  Phe Lys Phe 
    1010                 1015                 1020             


Thr Leu  Tyr Lys Asn Asp Leu  Ile Leu Ile Lys Asp  Thr Glu Thr 
    1025                 1030                 1035             


Gly Glu  Gln Gln Leu Phe Arg  Tyr Gly Ser Arg Asn  Asp Thr Ser 
    1040                 1045                 1050             


Lys His  Tyr Val Glu Leu Lys  Pro Tyr Glu Lys Ala  Lys Phe Glu 
    1055                 1060                 1065             


Gly Asn  Gln Gln Leu Met Asn  Leu Leu Gly Thr Val  Ala Lys Gly 
    1070                 1075                 1080             


Gly Gln  Cys Leu Lys Gly Ile  Asn Lys Pro Asn Leu  Ser Ile Tyr 
    1085                 1090                 1095             


Lys Val  Lys Thr Asp Val Leu  Gly Asn Lys Tyr Phe  Ile Lys Lys 
    1100                 1105                 1110             


Glu Gly  Asp Gln Pro Gln Leu  Asn Phe Lys Lys Lys  Phe 
    1115                 1120                 1125     


<210>  692
<211>  1126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  692

Met Ala Ser Lys His Glu Asn Leu Gly Ala Leu Thr Phe Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Val Leu Gly Glu Asn Arg Ile 
            20                  25                  30          


Ile Asp Leu Gly Val Arg Cys Phe Asp Lys Ala Glu Thr Ala Lys Glu 
        35                  40                  45              


Gly Glu Ser Leu Asn Leu Ala Arg Arg Thr Ala Arg Leu Leu Arg Arg 
    50                  55                  60                  


Arg Leu Arg Arg Arg Ala Trp Arg Leu Thr Lys Leu Ala Arg Leu Leu 
65                  70                  75                  80  


Lys Arg Glu Gly Leu Ile Ala Asp Thr Asn Val Phe Gln Lys Pro Phe 
                85                  90                  95      


Asn Leu Lys Asp Ala Asn Glu Lys Gly Leu Ser Phe Trp Gln Leu Arg 
            100                 105                 110         


Val Asp Ala Leu Asp Arg Lys Leu Ser Ala Glu Glu Trp Ala Arg Val 
        115                 120                 125             


Ile Tyr His Leu Cys Lys His Arg Gly Phe His Trp Ile Ser Arg Ala 
    130                 135                 140                 


Glu Glu Lys Ala Ala Glu Gly Asp Ser Lys Ser Glu Gly Gly Lys Val 
145                 150                 155                 160 


Lys Gln Gly Leu Ala Gly Thr Lys Lys Leu Met Gln Glu Lys Asn Tyr 
                165                 170                 175     


Arg Thr Ala Ala Glu Met Val Leu Thr Glu Phe Pro Glu Ala Gln Arg 
            180                 185                 190         


Asn Lys Gln Gly Asp Tyr Ser Lys Ala Leu Ser Arg Ile Leu Leu Gly 
        195                 200                 205             


Asp Glu Met Thr Gln Leu Phe Lys His Gln Arg Glu Leu Gly Asn Pro 
    210                 215                 220                 


His Ala Thr Glu Lys Leu Glu Ala Ala Ile Leu Gly Asn Gly Asp His 
225                 230                 235                 240 


Lys Ser Gly Leu Phe Trp Ala Gln Asn Pro Ala Leu Ala Gly Asn Asp 
                245                 250                 255     


Leu Leu Lys Met Leu Gly His Cys Thr Phe Glu Lys Ala Glu Tyr Arg 
            260                 265                 270         


Val Pro Lys Ala Ser Phe Thr Ala Glu Arg His Val Trp Leu Thr Arg 
        275                 280                 285             


Leu Asn Asn Leu Arg Ile Val Val Asp Gly Thr Thr Arg Pro Leu Asn 
    290                 295                 300                 


Glu Ala Glu Arg His Leu Ala Leu Leu Met Pro Tyr Gln Gln Ala Gly 
305                 310                 315                 320 


Asp Leu Thr Tyr Lys Gln Leu Arg Thr Ala Leu Thr Lys Ala Gly Tyr 
                325                 330                 335     


Leu Pro Glu Ser Phe Arg Tyr Thr Gly Leu Ala Tyr Ala Ser Gly Gln 
            340                 345                 350         


Lys Ala Asp Glu Lys Ala Lys Asp Pro Glu Ser Ala Pro Leu Val Lys 
        355                 360                 365             


Leu Pro Ala Trp His Glu Leu Arg Lys Thr Leu Glu Lys Lys Glu Leu 
    370                 375                 380                 


Ala Ser Glu Trp Gln Lys Ile Ser Ser Asp Ala Leu Asp Gly Lys Pro 
385                 390                 395                 400 


Gln Leu Leu Asp Gln Ile Ala Trp Val Leu Ser Val Tyr Lys Asp Asp 
                405                 410                 415     


Thr Glu Val Glu Ala Glu Leu Cys Lys Leu Asn Leu Pro Asn Ala Glu 
            420                 425                 430         


Lys Met Ile Asp Ala Leu Leu Asp Ile Arg Phe Asp Lys Phe Ser Asn 
        435                 440                 445             


Leu Ser Leu Lys Ala Leu Arg Gln Ile Val Pro Leu Met Glu Gln Gly 
    450                 455                 460                 


Gln Arg Tyr Asp Glu Ala Val Ala Asn Ile Pro Glu Tyr Gly His His 
465                 470                 475                 480 


Ser Gln Leu His Glu Val Gly Ala Gly Glu His Lys Tyr Leu Pro Pro 
                485                 490                 495     


Phe Tyr Lys Glu Arg Asp Lys Thr Gly Arg Met Val Phe Asn Asp Asp 
            500                 505                 510         


Met Asp Ile Pro Arg Asn Pro Val Val Leu Arg Ala Leu Asn Gln Ala 
        515                 520                 525             


Arg Lys Val Val Asn Ala Leu Ile Lys Glu Tyr Gly Ser Pro His Glu 
    530                 535                 540                 


Val His Ile Glu Met Ala Arg Asp Leu Ser Arg Pro Leu Asp Glu Arg 
545                 550                 555                 560 


Arg Asp Val Glu Lys Leu Gln Lys Glu Phe Arg Asp Arg Asn Glu Lys 
                565                 570                 575     


Asp Lys Ala Leu Phe Ala Gln Asp Phe Asn Ile Val Gly Ala Val Lys 
            580                 585                 590         


Gly Lys Glu Phe Glu Lys Tyr Gln Leu Tyr Arg Glu Gln Gln Gly Lys 
        595                 600                 605             


Cys Ala Tyr Ser Ile Glu Pro Met Asp Ile His Arg Leu Phe Glu Gln 
    610                 615                 620                 


Gly Tyr Val Glu Ile Asp His Ala Leu Pro Tyr Ser Arg Ser Phe Asp 
625                 630                 635                 640 


Asp Ser Lys Asn Asn Arg Val Leu Val Leu Ala Lys Glu Asn Arg Asp 
                645                 650                 655     


Lys Gly Asn Arg Thr Pro Phe Glu Tyr Leu Gly Gly Lys Asp Asn Ser 
            660                 665                 670         


Glu Arg Trp Arg Gln Phe Val Ala Phe Val Glu Gly Asn Lys Ser Tyr 
        675                 680                 685             


Arg Leu Ala Lys Arg Ser Arg Leu Leu Arg Lys Asp Phe Gly Lys Asp 
    690                 695                 700                 


Glu Ala Lys Gly Phe Arg Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ile 
705                 710                 715                 720 


Cys Arg Phe Phe Lys Asn Tyr Val Glu Gln Tyr Leu Gln Leu His Glu 
                725                 730                 735     


Asp Ser Asp Ala Lys Arg Cys Val Val Leu Ser Gly Gln Met Thr Ser 
            740                 745                 750         


Phe Leu Arg Ala Arg Trp Gly Leu Val Lys Val Arg Gly Asp Ser Asp 
        755                 760                 765             


Arg His His Ala Leu Asp Gly Ala Ile Val Ala Ala Cys Ser His Ser 
    770                 775                 780                 


Met Val Lys Arg Leu Ser Asp Tyr Ser Arg Arg Lys Glu Leu Glu Gln 
785                 790                 795                 800 


Val Arg Ala Gly Phe Val Asp Val Asp Thr Gly Glu Ile Val Asn Pro 
                805                 810                 815     


Ala Met Phe Gln Gln Val Gln Glu His Phe Pro Asn Pro Trp Pro His 
            820                 825                 830         


Phe Arg Asp Glu Val Lys Leu Arg Leu Asn Val Asp Asp Pro Ala Leu 
        835                 840                 845             


Leu His Ser Glu Met Asn Arg Phe Gly Thr Tyr Ser Pro Glu Ala Leu 
    850                 855                 860                 


Lys Ala Leu Arg Pro Leu Phe Ile Ser Arg Ala Pro Gln Arg Arg Asn 
865                 870                 875                 880 


Ser Gly Ala Ala His Lys Asp Thr Ile Tyr Ala Gln Pro Glu Arg Leu 
                885                 890                 895     


Val Ala Gln Gly Gly Val Thr Gln Lys Val Pro Leu Thr Ser Leu Thr 
            900                 905                 910         


Leu Lys Asp Leu Asp Lys Leu Ile Asp Pro His Arg Asn Glu Lys Leu 
        915                 920                 925             


Tyr Ser Ala Ile Arg Ile Arg Leu Glu Gln His Gly Gly Lys Gly Asp 
    930                 935                 940                 


Lys Ala Phe Pro Ala Ser Asn Pro Leu Arg Lys Pro Asp Ser Glu Gly 
945                 950                 955                 960 


Asn Pro Thr Gly Pro Ile Val Arg Gly Val Thr Met Val Ile Asp Lys 
                965                 970                 975     


Leu Ser Gly Ile Pro Ile Arg Gly Gly Ile Ala Lys Asn Asp Ser Met 
            980                 985                 990         


Val Arg Val Asp Val Phe Thr Lys  Ala Gly Lys Phe His  Leu Val Pro 
        995                 1000                 1005             


Val Tyr  Val His His Ala Val  Ala Lys Val Leu Pro  Asn Arg Ala 
    1010                 1015                 1020             


Val Val  Ala Tyr Lys Asp Glu  Thr Glu Trp Thr Leu  Ile Asp Asp 
    1025                 1030                 1035             


Gly Phe  Asp Phe Cys Phe Ser  Met Tyr Pro Asn Asp  Leu Leu Arg 
    1040                 1045                 1050             


Ile Lys  Leu Arg Asn Ala Ser  His Leu Gly Tyr Tyr  Ala Ser Cys 
    1055                 1060                 1065             


Asn Arg  Ser Thr Gly Ala Ile  Asn Leu Trp Ser His  Asp Arg Asn 
    1070                 1075                 1080             


Gln Gln  Val Gly Lys Asp Gly  Met Ile Glu Ser Ile  Gly Val Lys 
    1085                 1090                 1095             


Thr Ala  Leu Asn Val Glu Lys  Leu Asn Ile Asp Val  Leu Gly Asn 
    1100                 1105                 1110             


Arg Tyr  Pro Ala Pro Pro Glu  Lys Arg Arg Asp Leu  Ala 
    1115                 1120                 1125     


<210>  693
<211>  1127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  693

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Val Glu Arg Arg Ile 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn His Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Asn Val Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Glu Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ala Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Ser Tyr Leu Gln Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Thr Thr Lys Lys Asp Ser Glu Asp Glu Tyr Ile Thr Leu Asp Asn 
                245                 250                 255     


Ile Phe Gly Ile Leu Ile Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr 
            260                 265                 270         


Arg Ala Ala Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Glu 
    290                 295                 300                 


Glu Gln Lys Asn Gln Ile Ile Thr Asn Val Lys Asn Glu Lys Ala Met 
305                 310                 315                 320 


Gly Pro Ala Lys Leu Phe Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp 
                325                 330                 335     


Val Ala Asp Ile Lys Gly Tyr Arg Ile Asp Lys Ser Asp Lys Ala Glu 
            340                 345                 350         


Ile His Thr Phe Glu Ala Tyr Arg Lys Met Lys Thr Leu Ala Thr Ile 
        355                 360                 365             


Asp Ile Glu Gln Met Glu Arg Glu Asn Leu Asp Lys Leu Ala Tyr Val 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Lys Glu Gly Ile Gln Glu Ala Leu Glu His 
385                 390                 395                 400 


Glu Phe Ala Asp Gly Thr Phe Ser Gln Glu Gln Ile Asp Glu Leu Val 
                405                 410                 415     


Gln Phe Arg Lys Ala Asn Ser Ser Ile Phe Gly Lys Gly Trp His Ser 
            420                 425                 430         


Phe Ser Val Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Ala Thr 
        435                 440                 445             


Ser Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr 
    450                 455                 460                 


Thr Ser Ser Ser Asn Lys Thr Lys Tyr Ile Asp Glu Lys Gln Leu Thr 
465                 470                 475                 480 


Glu Glu Ile Tyr Asn Pro Val Ala Ala Lys Ser Val Arg Gln Ala Ile 
                485                 490                 495     


Lys Ile Val Asn Ala Ala Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile 
            500                 505                 510         


Val Ile Glu Met Ala Arg Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala 
        515                 520                 525             


Ile Gln Lys Ile Gln Lys Ala Asn Lys Asp Glu Lys Asp Ala Ala Met 
    530                 535                 540                 


Leu Lys Ala Ala Asn Gln Tyr Asn Gly Lys Ala Glu Leu Pro His Ser 
545                 550                 555                 560 


Val Phe His Gly His Lys Gln Leu Ala Thr Lys Ile Arg Leu Trp His 
                565                 570                 575     


Gln Gln Gly Glu Arg Cys Leu Tyr Thr Gly Lys Thr Ile Ser Ile His 
            580                 585                 590         


Asp Leu Ile Asn Asn Ser Asn Gln Phe Glu Ile Asp His Ile Leu Pro 
        595                 600                 605             


Leu Ser Ile Thr Phe Asp Asp Ser Leu Ala Asn Lys Val Leu Val Tyr 
    610                 615                 620                 


Ala Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu 
625                 630                 635                 640 


Asp Ser Met Asp Asp Ala Trp Ser Phe Arg Glu Leu Lys Ala Phe Val 
                645                 650                 655     


Arg Asp Ser Lys Ala Leu Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr 
            660                 665                 670         


Glu Glu Asp Ile Ser Lys Phe Asp Val Arg Lys Lys Phe Ile Glu Arg 
        675                 680                 685             


Asn Leu Val Asp Thr Arg Tyr Ala Ser Arg Val Val Leu Asn Ala Leu 
    690                 695                 700                 


Gln Glu His Phe Arg Val His Lys Thr Asp Thr Lys Val Ser Val Val 
705                 710                 715                 720 


Arg Gly Gln Phe Thr Ser Gln Leu Arg Arg His Trp Gly Ile Glu Lys 
                725                 730                 735     


Thr Arg Asp Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala 
            740                 745                 750         


Ala Ser Ser Gln Leu Asn Leu Trp Lys Lys Gln Lys Asn Thr Leu Val 
        755                 760                 765             


Ser Tyr Ser Glu Asp Gln Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile 
    770                 775                 780                 


Ser Asp Asp Glu Tyr Lys Glu Ser Val Phe Lys Ala Pro Tyr Gln His 
785                 790                 795                 800 


Phe Val Asp Thr Leu Lys Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe 
                805                 810                 815     


Ser Tyr Gln Val Asp Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr 
            820                 825                 830         


Ile Tyr Ala Thr Arg Gln Ala Lys Val Gly Lys Asp Lys Lys Asp Glu 
        835                 840                 845             


Thr Tyr Val Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Thr Gly Tyr 
    850                 855                 860                 


Asp Ala Phe Ile Lys Ile Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met 
865                 870                 875                 880 


Tyr Arg His Asp Pro Gln Thr Phe Glu Lys Val Ile Glu Pro Ile Leu 
                885                 890                 895     


Glu Asn Tyr Pro Asn Lys Glu Leu Asn Glu Lys Gly Lys Glu Val Pro 
            900                 905                 910         


Cys Asn Pro Phe Leu Lys Tyr Lys Glu Asp His Gly Tyr Ile Arg Lys 
        915                 920                 925             


Tyr Ser Lys Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr 
    930                 935                 940                 


Asp Ser Lys Leu Gly Asn His Ile Asp Ile Thr Pro Lys Asn Ser Asn 
945                 950                 955                 960 


Asn Lys Val Val Leu Gln Ser Val Ser Pro Trp Arg Ala Asp Val Tyr 
                965                 970                 975     


Phe Asn Lys Thr Thr Gly Lys Tyr Glu Ile Leu Gly Leu Lys Tyr Ala 
            980                 985                 990         


Asp Leu Lys Phe Glu Lys Gly Thr  Gly Thr Tyr Lys Ile  Ser Glu Glu 
        995                 1000                 1005             


Lys Tyr  Asn Asp Ile Lys Ile  Lys Glu Gly Val Asp  Ser Asp Ser 
    1010                 1015                 1020             


Glu Phe  Lys Phe Thr Leu Tyr  Lys Asn Asp Leu Leu  Leu Ile Lys 
    1025                 1030                 1035             


Asp Thr  Glu Thr Lys Glu Gln  Gln Leu Phe Arg Phe  Leu Ser Arg 
    1040                 1045                 1050             


Thr Met  Pro Asn Val Lys His  Tyr Val Glu Leu Lys  Pro Tyr Asp 
    1055                 1060                 1065             


Lys Gln  Lys Phe Asp Asp Asn  Glu Glu Leu Ile Lys  Ile Leu Gly 
    1070                 1075                 1080             


Ile Val  Ala Lys Gly Gly Gln  Cys Lys Lys Gly Val  Ser Lys Pro 
    1085                 1090                 1095             


Asn Ile  Ser Ile Tyr Lys Ile  Arg Thr Asp Val Leu  Gly Asn Gln 
    1100                 1105                 1110             


His Ile  Ile Lys Asn Glu Gly  Asp Lys Pro Lys Leu  Asp Phe 
    1115                 1120                 1125         


<210>  694
<211>  1127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  694

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asp Ser Gly Lys Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Thr 
        35                  40                  45              


Ser Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Lys His Arg Ser Val 
    50                  55                  60                  


Arg Leu His Asp Leu Phe Glu Glu Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Gln Ile Arg Val Gln Gly Leu 
                85                  90                  95      


Asp Lys Arg Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Ala Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Gln Gln Thr Pro Gly Gln Ile Gln Leu Asp Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Arg Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Lys Ile Thr 
        195                 200                 205             


Glu Gly Phe Ile Glu Asp Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Thr Thr Lys Lys Asp Ser Gln Gly Glu Tyr Ile Thr Leu Asp Asn 
                245                 250                 255     


Ile Phe Gly Ile Leu Ile Gly Lys Cys Thr Phe Tyr Pro Asp Glu Tyr 
            260                 265                 270         


Arg Ala Ser Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Val 
    290                 295                 300                 


Glu Gln Lys Lys Thr Ile Val Glu Tyr Ala Lys Thr Ala Lys Thr Leu 
305                 310                 315                 320 


Gly Ala Ser Thr Leu Leu Lys Tyr Ile Ala Lys Leu Ile Gly Val Ser 
                325                 330                 335     


Val Asp Gln Ile His Gly Tyr Arg Ile Asp Pro Asn Glu Lys Pro Glu 
            340                 345                 350         


Met His Thr Phe Glu Val Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile 
        355                 360                 365             


Arg Val Glu Glu Leu Pro Arg Lys Val Ile Asp Glu Leu Ala His Ile 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Arg Glu Gly Ile Glu Glu Ala Ile Asn Ala 
385                 390                 395                 400 


Lys Leu Lys Asp Ile Phe Ser Gln Asp Gln Val Leu Glu Leu Val Gln 
                405                 410                 415     


Phe Arg Lys Asn Asn Ser Ser Leu Phe Ser Lys Gly Trp His Asn Phe 
            420                 425                 430         


Ser Leu Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser 
        435                 440                 445             


Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys 
    450                 455                 460                 


Glu Thr Ser Lys Arg Thr Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu 
465                 470                 475                 480 


Glu Ile Tyr Asn Pro Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys 
                485                 490                 495     


Ile Ile Asn Glu Ala Thr Lys Arg Tyr Gly Ile Phe Asp Asn Ile Val 
            500                 505                 510         


Ile Glu Met Ala Arg Glu Asn Asn Glu Glu Asp Ala Lys Lys Glu Tyr 
        515                 520                 525             


Ile Lys Arg Gln Lys Ala Asn Leu Asp Glu Lys Asn Ala Ala Met Glu 
    530                 535                 540                 


Lys Ala Ala Phe Gln Tyr Asn Gly Lys Lys Glu Leu Pro Asp Asn Val 
545                 550                 555                 560 


Phe His Gly His Lys Glu Leu Ala Thr Lys Ile Arg Leu Trp His Gln 
                565                 570                 575     


Gln Gly Glu Lys Cys Leu Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp 
            580                 585                 590         


Leu Ile Gln Asn Arg Tyr Lys Tyr Glu Ile Asp His Ile Leu Pro Leu 
        595                 600                 605             


Ser Leu Ser Phe Asp Asp Ser Leu Ser Asn Lys Val Leu Val Leu Ala 
    610                 615                 620                 


Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp 
625                 630                 635                 640 


Ser Met Asp Tyr Ala Trp Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys 
                645                 650                 655     


Asp Ser Lys Leu Leu Ser Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu 
            660                 665                 670         


Glu Asp Ile Ser Lys Ile Glu Val Lys Gln Lys Phe Ile Glu Arg Asn 
        675                 680                 685             


Leu Val Asp Thr Arg Tyr Ser Ser Arg Val Val Leu Asn Ala Leu Gln 
    690                 695                 700                 


Asp Phe Tyr Lys Glu His Gln Phe Asp Thr Thr Ile Ser Val Val Arg 
705                 710                 715                 720 


Gly Gln Phe Thr Ser Gln Leu Arg Arg Lys Trp Gly Ile Glu Lys Ser 
                725                 730                 735     


Arg Glu Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala Ala 
            740                 745                 750         


Ser Ser Gln Leu Arg Leu Trp Lys Lys His Asn Asn Pro Leu Ile Ser 
        755                 760                 765             


Tyr Lys Glu Glu Gln Phe Val Asp Ser Glu Thr Gly Glu Ile Ile Ser 
    770                 775                 780                 


Met Ser Asp Asp Glu Tyr Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp 
785                 790                 795                 800 


His Phe Val Asp Thr Leu Lys Ser Lys Lys Phe Glu Asp Ser Ile Leu 
                805                 810                 815     


Phe Ser Tyr Gln Val Asp Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala 
            820                 825                 830         


Thr Ile Tyr Ala Thr Arg Lys Ala Lys Leu Asp Lys Asp Lys Ser Glu 
        835                 840                 845             


Glu Thr Tyr Val Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Ala Gly 
    850                 855                 860                 


Tyr Asp Thr Phe Ile Lys Ile Tyr Asn Lys Asp Lys Ser Arg Phe Leu 
865                 870                 875                 880 


Met Tyr His Lys Asp Pro Gln Thr Phe Glu Lys Val Ile Glu Glu Ile 
                885                 890                 895     


Leu Arg Thr Tyr Pro Ser Lys Glu Leu Asn Glu Lys Asn Lys Glu Val 
            900                 905                 910         


Pro Cys Asn Pro Phe Glu Lys Tyr Arg Gln Glu Asn Gly Pro Ile Arg 
        915                 920                 925             


Lys Tyr Ser Arg Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr 
    930                 935                 940                 


Tyr Asp Asn Lys Leu Gly Asn His Ile Asp Ile Thr Pro Asn Glu Ser 
945                 950                 955                 960 


Met Asn Gln Val Val Leu Gln Ser Leu Lys Pro Trp Arg Thr Asp Val 
                965                 970                 975     


Phe Phe Asn His Gln Thr Glu Lys Tyr Glu Leu Met Gly Leu Lys Tyr 
            980                 985                 990         


Ser Asp Leu Asn Phe Glu Lys Gly  Ser Gly Lys Tyr Ser  Ile Ser Leu 
        995                 1000                 1005             


Glu Lys  Tyr Asn Ser Ile Lys  Met Asn Glu Gly Val  Ser Glu Glu 
    1010                 1015                 1020             


Ser Glu  Phe Lys Phe Thr Leu  Tyr Lys Asn Asp Leu  Ile Leu Ile 
    1025                 1030                 1035             


Lys Asp  Ala Leu Thr Lys Glu  Ala Gln Ile Phe Arg  Tyr Gly Ser 
    1040                 1045                 1050             


Arg Asn  Asp Thr Ser Lys His  Tyr Val Glu Leu Lys  Pro Tyr Asp 
    1055                 1060                 1065             


Met Ser  Lys Phe Glu Gly Asp  Glu Arg Leu Ile Lys  Val Leu Gly 
    1070                 1075                 1080             


Thr Val  Ala Lys Gly Gly Arg  Cys Ile Lys Gly Leu  Asn Lys Lys 
    1085                 1090                 1095             


Asp Ile  Ser Ile Phe Lys Val  Lys Thr Asp Val Leu  Gly Asn Lys 
    1100                 1105                 1110             


His Phe  Ile Lys Lys Glu Gly  Glu Glu Pro Lys Leu  Lys Phe 
    1115                 1120                 1125         


<210>  695
<211>  1128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  695

Met Thr Lys Leu Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg 
            20                  25                  30          


Leu Lys Gly Lys Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys 
        35                  40                  45              


Ala Ala Ala Lys Arg Arg Ala Gly Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Asn Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His 
65                  70                  75                  80  


Ile Thr Pro Thr Asp Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Ala Arg Tyr Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Gln Gln Tyr Ser Thr Met Tyr 
        115                 120                 125             


His Leu Arg Asn Arg Leu Met Thr Asp Pro Ser Arg Ala Asp Val Arg 
    130                 135                 140                 


Glu Ile Tyr Phe Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Asp Ala Lys Asp Phe Asn Thr Asn Asp Val Ala Leu 
                165                 170                 175     


Asp Glu Ile Phe Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Gly Ile Thr Phe Asp Glu Asp Lys Ala Asn Glu Phe Lys Thr 
        195                 200                 205             


Ile Leu Leu Asn Glu Ile Ala Thr Ser Ile Asp Thr Gln Arg Ala Leu 
    210                 215                 220                 


Val Lys Leu Leu Leu Ala Glu Glu Asp Asp Lys Asp Ile Leu Lys Gln 
225                 230                 235                 240 


Gln Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Val Val Gly Leu Lys 
                245                 250                 255     


Thr Lys Leu Asn Leu Ala Leu Gly Thr Glu Val Asp Ser Ser Glu Ala 
            260                 265                 270         


Thr Ala Trp Asn Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp Ala Gly 
        275                 280                 285             


Ile Glu Ser Ala Met Thr Asp Glu Gly Thr Glu Ile Leu Asp Gln Ile 
    290                 295                 300                 


Arg Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Thr Gly 
305                 310                 315                 320 


Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Thr Gln His His Glu 
                325                 330                 335     


Asp Leu Gln Leu Phe Lys Ala Tyr Leu Lys Gln Leu Glu Asp Asp Gly 
            340                 345                 350         


Thr Ala Lys Ala Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp Gly Asp 
        355                 360                 365             


Asp Ala Ala Pro Phe Leu Arg Glu Asn Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Asp Val Thr Ala His Pro Asn Thr Lys Ser Pro Glu Leu Leu Glu Arg 
385                 390                 395                 400 


Leu Ala Gln Pro Asp Phe Met Leu Lys Gln Arg Asn Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Gln Ile Ile Lys 
            420                 425                 430         


Asn Gln Ala Val Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Lys Ser Met Pro Tyr Gln Leu Asp Glu Leu Leu Asn Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Ala Lys Glu Gln Lys 
465                 470                 475                 480 


Ala Ala Gln Gly Gly Val Phe Ala Trp Met Val Arg Lys Asp Pro Glu 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Arg Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asn Glu Lys Leu 
545                 550                 555                 560 


Ser Ile Glu Gln Lys Gln Gln Val Ile Arg Glu Leu Phe Glu Arg His 
                565                 570                 575     


Asn Ser Val Thr Ile Lys Gln Phe Ala Glu Asn Leu Arg Ala His Gly 
            580                 585                 590         


Asp Tyr Ala His Ile Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr Arg Gln Leu Lys Ser Leu Leu Pro 
    610                 615                 620                 


Glu Ala Ile Asp Asp Pro Ala Lys Gln Ala Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Ala Trp Ser Thr Val Phe Glu Asp Ala Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Lys Glu Ile Ser Trp Leu Gly Ser Glu Ala Ile Thr Lys Leu Ser Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Arg Lys Phe Leu Asn Gly 
        675                 680                 685             


Leu Thr Leu Gly Asn Gly His Thr Ile Ile Gln Glu Leu Leu Leu Ser 
    690                 695                 700                 


Thr Asn Asn Leu Met Gln Ile Leu Thr Asp Glu Thr Leu Gln Lys Lys 
705                 710                 715                 720 


Met Thr Glu Leu Asn Ala Asp Lys Leu Lys Thr Ala Asn Ile Asn Asp 
                725                 730                 735     


Ala Ile Asp Asn Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Arg Val Val Asp Asp Ile Lys Arg Ala Ala Asp Gly Gln 
        755                 760                 765             


Asp Pro Ser Trp Leu Tyr Val Glu Thr Ala Asp Gly Gly Gly Thr Pro 
    770                 775                 780                 


Gly Lys Arg Thr Arg Ala Arg Gln His Gln Leu Gln Glu Ile Tyr Ala 
785                 790                 795                 800 


Asn Ala Ala His Glu Leu Ile Asp Thr Ala Val Arg Gly Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Ser Asp Lys Ala Asp Phe Asn Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Ala Pro Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val His Ala Ile Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Ala Thr Phe Ala Ser Thr Ile Tyr Ala Gln Lys 
                885                 890                 895     


Met Asn Ala Thr Trp Arg Gln Trp His Glu Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Arg Asn Leu Gln Met Arg Pro Asp Gln Ile Asp Lys Tyr 
        915                 920                 925             


Ala Ser Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Val Ala Ala Gln Tyr Pro Asp Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu Leu Glu 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Phe Pro Asn 
        995                 1000                 1005             


Leu Gln  Ala Phe Phe Thr Tyr  Gly Lys Phe Thr Lys  Ala Asp Val 
    1010                 1015                 1020             


Lys Lys  Leu Arg Gly Phe Asn  Phe Ile Arg Asp Ile  Thr His Ala 
    1025                 1030                 1035             


Glu Asp  Lys Ile Val Ala Lys  Asp Thr Gly Glu Val  Val Trp Asp 
    1040                 1045                 1050             


Lys Gln  Arg Asp Val Asp Glu  Leu Asp Arg Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val Arg Phe Glu Thr  Ala Asp Leu 
    1070                 1075                 1080             


Phe Lys  Gln Thr Val Tyr Gly  Ala Arg Asp Ser Lys  Glu Ala Gly 
    1085                 1090                 1095             


Gly Ser  Lys Gln Leu Ile Pro  Lys Lys Lys Asp Ile  Gln Leu Ile 
    1100                 1105                 1110             


Phe Met  Gly Ala Ile Ser Glu  Arg Thr Arg Pro Ile  Leu Pro Leu 
    1115                 1120                 1125             


<210>  696
<211>  1128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  696

Met Asp Glu Lys Met Asp Tyr Arg Ile Gly Leu Asp Ile Gly Ile Ala 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Leu Gln Asn Asn Ser Asp Asp Glu Pro Val 
            20                  25                  30          


Arg Ile Val Asp Leu Gly Val Arg Ile Phe Asp Thr Ala Glu Ile Pro 
        35                  40                  45              


Lys Thr Gly Glu Ser Leu Ala Gly Pro Arg Arg Ala Ala Arg Thr Thr 
    50                  55                  60                  


Arg Arg Arg Leu Arg Arg Arg Lys His Arg Leu Asp Arg Ile Lys Trp 
65                  70                  75                  80  


Leu Phe Glu Asn Gln Gly Leu Ile Asn Ile Asp Asp Phe Leu Lys Arg 
                85                  90                  95      


Tyr Asn Met Ala Gly Leu Pro Asp Val Tyr Gln Leu Arg Tyr Glu Ala 
            100                 105                 110         


Leu Asp Arg Lys Leu Thr Asp Glu Glu Leu Ala Gln Val Leu Leu His 
        115                 120                 125             


Ile Ala Lys His Arg Gly Phe Arg Ser Thr Arg Lys Ala Glu Thr Ala 
    130                 135                 140                 


Ala Lys Glu Asn Gly Ala Val Leu Lys Ala Thr Asp Glu Asn Gln Lys 
145                 150                 155                 160 


Arg Met Gln Glu Lys Gly Tyr Arg Thr Val Gly Glu Met Ile Tyr Leu 
                165                 170                 175     


Asp Glu Ala Phe Arg Ile Gly Cys Ser Trp Ser Glu Lys Gly Tyr Ile 
            180                 185                 190         


Leu Thr Pro Arg Asn Lys Ala Glu Asn Tyr Gln His Thr Met Leu Arg 
        195                 200                 205             


Ala Met Leu Val Glu Glu Val Lys Glu Ile Phe Ser Ser Gln Arg Arg 
    210                 215                 220                 


Leu Gly Asn Lys Lys Ala Thr Glu Glu Leu Glu Glu Lys Tyr Leu Glu 
225                 230                 235                 240 


Ile Met Thr Ser Gln Arg Ser Phe Asp Leu Gly Pro Gly Met Gln Pro 
                245                 250                 255     


Asp Gly Lys Pro Ser Pro Tyr Ala Met Glu Gly Phe Ser Asp Arg Val 
            260                 265                 270         


Gly Lys Cys Thr Phe Leu Gly Asp Gln Gly Glu Leu Arg Gly Ala Lys 
        275                 280                 285             


Gly Thr Tyr Thr Ala Glu Tyr Phe Val Ala Leu Gln Lys Ile Asn His 
    290                 295                 300                 


Thr Lys Leu Val Asn Gln Asp Gly Glu Thr Arg Asn Phe Thr Glu Glu 
305                 310                 315                 320 


Glu Arg Arg Ala Leu Thr Leu Leu Leu Phe Thr Gln Lys Glu Val Lys 
                325                 330                 335     


Tyr Ala Ala Val Arg Lys Lys Leu Gly Leu Pro Glu Asp Ile Leu Phe 
            340                 345                 350         


Tyr Asn Leu Asn Tyr Lys Lys Ala Ala Thr Lys Glu Glu Gln Gln Lys 
        355                 360                 365             


Glu Asn Gln Asn Thr Glu Lys Ala Lys Phe Ile Gly Met Pro Tyr Tyr 
    370                 375                 380                 


His Asp Tyr Lys Lys Cys Leu Glu Glu Arg Val Lys Tyr Leu Thr Glu 
385                 390                 395                 400 


Asn Glu Val Arg Asp Leu Phe Asp Glu Ile Gly Met Ile Leu Thr Cys 
                405                 410                 415     


Tyr Lys Asn Asp Asp Ser Arg Thr Glu Arg Leu Ala Lys Leu Gly Leu 
            420                 425                 430         


Val Pro Ile Glu Met Glu Gly Leu Leu Ala Tyr Thr Pro Thr Lys Phe 
        435                 440                 445             


Gln His Leu Ser Met Lys Ala Met Arg Asn Ile Ile Pro Phe Leu Glu 
    450                 455                 460                 


Lys Gly Met Thr Tyr Asp Lys Ala Cys Glu Glu Ala Gly Tyr Asp Phe 
465                 470                 475                 480 


Lys Ala Asp Ser Lys Gly Thr Lys Gln Lys Leu Leu Thr Gly Glu Asn 
                485                 490                 495     


Val Asn Gln Thr Ile Asn Glu Ile Thr Asn Pro Val Val Lys Arg Ser 
            500                 505                 510         


Val Ser Gln Thr Val Lys Val Ile Asn Ala Ile Ile Arg Thr Tyr Gly 
        515                 520                 525             


Ser Pro Gln Ala Ile Asn Ile Glu Leu Ala Arg Glu Met Ser Lys Thr 
    530                 535                 540                 


Phe Glu Glu Arg Arg Lys Ile Lys Gly Asp Met Glu Lys Arg Gln Lys 
545                 550                 555                 560 


Asn Asn Glu Asp Val Lys Lys Gln Ile Gln Glu Leu Gly Lys Leu Ser 
                565                 570                 575     


Pro Thr Gly Gln Asp Ile Leu Lys Tyr Arg Leu Trp Gln Glu Gln Gln 
            580                 585                 590         


Gly Ile Cys Met Tyr Ser Gly Lys Thr Ile Pro Leu Glu Glu Leu Phe 
        595                 600                 605             


Lys Pro Gly Tyr Asp Ile Asp His Ile Leu Pro Tyr Ser Ile Thr Phe 
    610                 615                 620                 


Asp Asp Ser Phe Arg Asn Lys Val Leu Val Thr Ser Gln Glu Asn Arg 
625                 630                 635                 640 


Gln Lys Gly Asn Arg Thr Pro Tyr Glu Tyr Met Gly Asn Asp Glu Gln 
                645                 650                 655     


Arg Trp Asn Glu Phe Glu Thr Arg Val Lys Thr Thr Ile Arg Asp Tyr 
            660                 665                 670         


Lys Lys Gln Gln Lys Leu Leu Lys Lys His Phe Ser Glu Glu Glu Arg 
        675                 680                 685             


Ser Glu Phe Lys Glu Arg Asn Leu Thr Asp Thr Lys Tyr Ile Thr Thr 
    690                 695                 700                 


Val Ile Tyr Asn Met Ile Arg Gln Asn Leu Glu Met Ala Pro Leu Asn 
705                 710                 715                 720 


Arg Pro Glu Lys Lys Lys Gln Val Arg Ala Val Asn Gly Ala Ile Thr 
                725                 730                 735     


Ala Tyr Leu Arg Lys Arg Trp Gly Leu Pro Gln Lys Asn Arg Glu Thr 
            740                 745                 750         


Asp Thr His His Ala Met Asp Ala Val Val Ile Ala Cys Cys Thr Asp 
        755                 760                 765             


Gly Met Ile Gln Lys Ile Ser Arg Tyr Thr Lys Val Arg Glu Arg Cys 
    770                 775                 780                 


Tyr Ser Lys Gly Thr Glu Phe Val Asp Ala Glu Thr Gly Glu Ile Phe 
785                 790                 795                 800 


Arg Pro Glu Asp Tyr Ser Arg Ala Glu Trp Asp Glu Ile Phe Gly Val 
                805                 810                 815     


His Ile Pro Lys Pro Trp Glu Thr Phe Arg Ala Glu Leu Asp Val Arg 
            820                 825                 830         


Met Gly Asp Asp Pro Lys Gly Phe Leu Asp Thr His Ser Asp Val Ala 
        835                 840                 845             


Leu Glu Leu Asp Tyr Pro Glu Tyr Ile Tyr Glu Asn Leu Arg Pro Ile 
    850                 855                 860                 


Phe Val Ser Arg Met Pro Asn His Lys Val Thr Gly Ala Ala His Ala 
865                 870                 875                 880 


Asp Thr Ile Arg Ser Pro Arg His Phe Lys Asp Glu Gly Ile Val Leu 
                885                 890                 895     


Thr Lys Thr Ala Leu Thr Asp Leu Lys Leu Asp Lys Asp Gly Glu Ile 
            900                 905                 910         


Asp Gly Tyr Tyr Asn Pro Gln Ser Asp Leu Leu Leu Tyr Glu Ala Leu 
        915                 920                 925             


Lys Lys Gln Leu Leu Leu Tyr Gly Asn Asp Ala Lys Lys Ala Phe Ala 
    930                 935                 940                 


Gln Asp Phe His Lys Pro Lys Ala Asp Gly Thr Glu Gly Pro Val Val 
945                 950                 955                 960 


Arg Lys Val Lys Ile Gln Lys Lys Gln Thr Met Gly Val Phe Val Asp 
                965                 970                 975     


Ser Gly Asn Gly Ile Ala Glu Asn Gly Gly Met Val Arg Ile Asp Val 
            980                 985                 990         


Phe Arg Val Asn Gly Lys Tyr Tyr  Phe Val Pro Val Tyr  Thr Ala Asp 
        995                 1000                 1005             


Val Val  Lys Lys Val Leu Pro  Asn Arg Ala Ser Thr  Ala Asn Lys 
    1010                 1015                 1020             


Pro Tyr  Ser Glu Trp Arg Thr  Met Glu Asp Lys Asp  Phe Leu Phe 
    1025                 1030                 1035             


Ser Leu  Tyr Ser Arg Asp Leu  Val His Ile Lys Ser  Lys Lys Gly 
    1040                 1045                 1050             


Ile Pro  Ile Lys Asn Val Asp  Gly Ser Leu Gly Glu  Ser Lys Glu 
    1055                 1060                 1065             


Val Tyr  Ala Tyr Tyr Ile Gly  Ala Asp Ile Ser Gly  Ala Lys Ile 
    1070                 1075                 1080             


Glu Gly  Lys Ala His Asp Ser  Arg Tyr Thr Phe Arg  Gly Ile Gly 
    1085                 1090                 1095             


Val Gln  Lys Leu Glu Leu Ile  Glu Lys Cys Gln Val  Asp Ile Leu 
    1100                 1105                 1110             


Gly His  Ile Ser Thr Val Lys  Asn Glu Lys Arg Met  Gly Phe Ser 
    1115                 1120                 1125             


<210>  697
<211>  1128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  697

Met Asn Asp Lys Asn Gln Lys Tyr Arg Leu Gly Leu Asp Leu Gly Thr 
1               5                   10                  15      


Asn Ser Ile Gly Trp Cys Ile Tyr Ser Leu Asp Val Asn Asn Lys Pro 
            20                  25                  30          


Glu Thr Leu Ile Asp Met Gly Val Arg Ile Phe Ser Asp Gly Arg Asp 
        35                  40                  45              


Pro Lys Thr Lys Glu Pro Leu Ala Val Glu Arg Arg Thr Ala Arg Gly 
    50                  55                  60                  


Leu Arg Arg Ile Leu His Arg Arg Lys Leu Arg Arg Arg Gln Met Phe 
65                  70                  75                  80  


Arg Leu Leu Gln Glu Gln Gly Leu Met Pro Asn Asp Ala Ser Glu Ala 
                85                  90                  95      


Ala Ala Leu Lys Ser Leu Asn Pro Tyr Glu Leu Arg Val Lys Ala Leu 
            100                 105                 110         


Asp Thr Lys Leu Glu Pro Tyr Glu Leu Gly Arg Ala Leu Phe Asn Leu 
        115                 120                 125             


Ser Val Arg Arg Gly Phe Lys Ser Asn Arg Lys Glu Ala Val Gln Glu 
    130                 135                 140                 


Lys Glu Lys Asp Ser Gln Asn Asp Ala Lys Ser Gln Asn Glu Lys Cys 
145                 150                 155                 160 


Glu His Leu Ala Lys Ala Ile Lys Asp Ser Gly Cys Arg Thr Leu Gly 
                165                 170                 175     


Glu Phe Leu Trp Lys His Lys Asn Thr Glu Thr Gln Gln Thr Glu Asn 
            180                 185                 190         


Asp Ala Arg Asn Ile Gly Met Arg Phe Val Pro Gly Arg Ser Asn Tyr 
        195                 200                 205             


Tyr Pro Thr Arg Arg Met Tyr Val Glu Glu Phe Asp Ala Ile Arg Lys 
    210                 215                 220                 


Ala Gln Glu Pro Tyr Tyr Ser Gly Val Asn Trp Asp Ala Leu His Glu 
225                 230                 235                 240 


Lys Leu Phe Tyr Gln Arg Pro Leu Arg Ala Gln Glu Arg Gly Lys Cys 
                245                 250                 255     


Arg Tyr Met Pro Asp Lys Glu Arg Thr Phe Lys Ala Met Pro Cys Ser 
            260                 265                 270         


Gln Lys Ile Arg Ile Leu Gln Glu Val Tyr Asn Met Asp Tyr Ile Asp 
        275                 280                 285             


Glu Tyr Gly Lys Thr Ile Pro Leu Glu Glu Ala Gln Thr Asp Ser Leu 
    290                 295                 300                 


Ile Ser Leu Leu Asp Lys Arg Glu Lys Leu Ser Phe Lys Ala Met Lys 
305                 310                 315                 320 


Lys Glu Leu Gly Ile Asp Glu Ser Cys Thr Phe Asn Leu Glu Arg Gly 
                325                 330                 335     


Gly Arg Glu Tyr Leu Gln Gly Asn Ala Ala Ala Val Lys Leu Arg Asn 
            340                 345                 350         


Lys Lys Arg Phe Gly Glu Leu Trp Asp Ser Leu Ser Leu Ala Lys Gln 
        355                 360                 365             


Asp Ala Ile Val Glu Lys Leu Ile Thr Ala Asp Glu Asp Ala Glu Ile 
    370                 375                 380                 


Ile Ser Met Leu Ala Gln Tyr Asn Leu Thr Asp Glu Gln Lys Lys Asn 
385                 390                 395                 400 


Ile Ala Val Thr Val Leu Pro Ser Gly Thr Ala Met Leu Cys Lys Glu 
                405                 410                 415     


Val Thr Glu Met Leu Val Gln Lys Met Glu Gln Asn Lys Ile Pro Leu 
            420                 425                 430         


Thr Val Ala Val Gln Met Leu Gly Tyr Thr Tyr Ala Asp Gln Ser Val 
        435                 440                 445             


Arg Glu Glu Asp Thr Leu Pro Tyr Tyr Gly Ala Ile Leu Thr Gly Ser 
    450                 455                 460                 


Thr Met Gly Ala His Pro Glu Ala Asp Glu Ser Glu Pro Glu Leu Lys 
465                 470                 475                 480 


Tyr Gly Lys Ile Ser Asn Pro Thr Val His Val Ala Leu Asn Gln Thr 
                485                 490                 495     


Arg Thr Val Val Asn Ala Leu Ile Lys Ala Tyr Gly Lys Pro Gln Gln 
            500                 505                 510         


Ile Val Val Glu Leu Ser Arg Asp Leu Lys Ala Ser Arg Glu Ala Lys 
        515                 520                 525             


Ala Arg Ile Gln Lys Thr Ile Thr Met Asn Gln Lys Arg Asn Glu Arg 
    530                 535                 540                 


Asp Asn Lys Asn Ile Thr Asp Ile Thr Lys Ile Pro Tyr Pro Asn Arg 
545                 550                 555                 560 


Asn Asp Arg Leu Lys Tyr Arg Leu Trp Glu Glu Leu Gly Ser Gly Ser 
                565                 570                 575     


Asn Ser Phe Ser Arg Lys Cys Leu Tyr Cys Gly Lys Pro Ile Ser Gly 
            580                 585                 590         


Ser Glu Ile Phe Thr Glu Asn Ile Glu Ile Glu His Ile Leu Pro Phe 
        595                 600                 605             


Gly Arg Thr Leu Phe Asp Gly Glu Thr Asn Lys Thr Val Ala His Thr 
    610                 615                 620                 


Asp Cys Asn Arg Asp Lys Gly Asp Arg Ser Pro Phe Glu Ala Phe Gly 
625                 630                 635                 640 


Ser Ser Pro Asn Gly Tyr Asn Trp His Glu Ile Cys Ala Arg Ala Asn 
                645                 650                 655     


Ser Leu Lys Asn Pro Ala Lys Arg Thr Leu Phe Ala Glu Asn Ala Met 
            660                 665                 670         


Glu Lys Phe Glu Lys Asp Asn Ser Phe Ile Gln Arg Gln Leu Thr Asp 
        675                 680                 685             


Asn Ala Tyr Leu Ser Arg Ser Ala Leu Arg Tyr Leu Arg Cys Ile Cys 
    690                 695                 700                 


Lys Asp Val Trp Ser Ile Asn Gly Gly Met Thr Lys Leu Leu Arg Asp 
705                 710                 715                 720 


Lys Trp Asn Ile Asp Ser Ile Leu Lys Arg Lys Ile Asp Asp Pro Glu 
                725                 730                 735     


Ile Ala His Phe Glu Leu Lys Asn Asp Gln Ile Gly Gln Tyr Lys Lys 
            740                 745                 750         


Asn Arg Tyr Asp His Arg His His Ala Leu Asp Ala Ser Val Ile Ala 
        755                 760                 765             


Leu Ile Asp Arg Ala Met Val Gln Glu Ile Ser Gln Leu Asn Lys Ile 
    770                 775                 780                 


Arg Leu Lys Asn Arg Ile Glu Ala Pro Gln Met Pro Val Leu Arg Ser 
785                 790                 795                 800 


Glu Leu Ile Glu Lys Val Lys His Ile Thr Val Ser Phe Lys Pro Asp 
                805                 810                 815     


His Gly Arg Gln Gly Lys Leu Ser Lys Glu Thr Leu Leu Gly Lys Ile 
            820                 825                 830         


Lys Lys Ile Glu Lys Val Asp Ile Asp Ser Ile Gln Thr Asp Asp Ile 
        835                 840                 845             


Ala Ser Ile Lys Asp Asp Ala Ile Arg Thr Arg Phe Glu Thr Lys Arg 
    850                 855                 860                 


Arg Glu Leu Gly Asp Ile Thr Lys Ala Lys Ala Ala Leu Lys Thr Glu 
865                 870                 875                 880 


Phe Pro Lys Leu Asp Val Phe Lys Thr Tyr Tyr Val Ser Arg Thr Pro 
                885                 890                 895     


Ile Val Ser Leu Thr Glu Lys Asn Ile Asn Ser Ile Val Asp Glu Lys 
            900                 905                 910         


Ile Lys Glu Lys Leu Lys Thr Phe Ile Lys Glu His Ala Asp Leu Ser 
        915                 920                 925             


Phe Ala Glu Gln Leu Gln Lys Phe Ser Glu Thr Glu Trp Ile Gly Lys 
    930                 935                 940                 


Pro Ser Ala Ala Gly Lys Pro Gly Lys Lys Tyr Arg Ile Glu Lys Val 
945                 950                 955                 960 


Arg Cys Ile Asn Trp Gly Gln Thr Pro Ile Thr Ile Thr Ser Ser Ala 
                965                 970                 975     


Val Pro Arg Tyr Leu Cys Pro Glu Asp Tyr Leu Ala Ala Val Val Trp 
            980                 985                 990         


Gln Ile Pro Ser Lys Lys Lys Asp  Thr Glu Pro Glu Tyr  Lys Ala Thr 
        995                 1000                 1005             


Phe Leu  Arg Arg Asp Glu Phe  Asp Val Asn Asn Lys  Pro Lys Lys 
    1010                 1015                 1020             


Leu Glu  Lys Pro His Asp Ala  Ala Gln Tyr Ile Cys  Met Leu Tyr 
    1025                 1030                 1035             


Lys Asp  Asp Tyr Leu Glu Phe  Thr Glu Ser Gly Ala  Thr His Leu 
    1040                 1045                 1050             


Cys Arg  Ile Ala Gly Leu Pro  Ala Ser Gln Asn Asn  Lys Ile Asp 
    1055                 1060                 1065             


Ile Arg  Pro Ile Tyr Ala Val  Ser Asp Cys Lys Asp  Trp Ile Ile 
    1070                 1075                 1080             


Ala Thr  Asn Glu Asn Met Leu  Glu Pro Cys Trp Lys  Pro Gln Lys 
    1085                 1090                 1095             


Glu Glu  Lys Arg Ile Ser Val  Asn Val Leu Phe Gly  Lys His Lys 
    1100                 1105                 1110             


Ala Arg  Ser Val Thr Val Asn  Pro Ile Gly Arg Val  Phe Arg Lys 
    1115                 1120                 1125             


<210>  698
<211>  1128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  698

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Val Glu Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn His Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Ile Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Glu Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Lys Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Thr Thr Lys Lys Asp Ser Glu Asn Glu Tyr Ile Thr Leu Asp Asn 
                245                 250                 255     


Ile Phe Gly Ile Leu Ile Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr 
            260                 265                 270         


Arg Ala Ala Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Glu 
    290                 295                 300                 


Glu Gln Lys Asn Gln Ile Ile Asn Tyr Val Lys Asn Glu Lys Ala Met 
305                 310                 315                 320 


Gly Pro Ala Lys Leu Phe Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp 
                325                 330                 335     


Val Ala Asp Ile Lys Gly Tyr Arg Ile Asp Lys Ser Asp Lys Ala Glu 
            340                 345                 350         


Ile His Thr Phe Glu Ala Tyr Arg Lys Met Lys Thr Leu Glu Thr Ile 
        355                 360                 365             


Asp Phe Glu Lys Met Ser Arg Asp Gln Leu Asp Lys Leu Ala Tyr Val 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Arg Glu Gly Ile Gln Glu Ala Leu Asp His 
385                 390                 395                 400 


Glu Phe Ala Asp Gly Asn Phe Ser Gln Glu Gln Ile Asp Glu Leu Val 
                405                 410                 415     


Gln Phe Arg Lys Ala Asn Ser Ser Ile Phe Gly Lys Gly Trp His Asn 
            420                 425                 430         


Phe Ser Val Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Ala Thr 
        435                 440                 445             


Ser Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr 
    450                 455                 460                 


Thr Ser Ser Ser Asn Lys Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr 
465                 470                 475                 480 


Glu Glu Ile Tyr Asn Pro Val Val Ala Lys Ser Val Arg Gln Ala Ile 
                485                 490                 495     


Lys Ile Val Asn Ala Ala Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile 
            500                 505                 510         


Val Ile Glu Met Ala Arg Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala 
        515                 520                 525             


Ile Gln Lys Ile Gln Lys Ala Asn Lys Asp Glu Lys Asp Ala Ala Met 
    530                 535                 540                 


Leu Lys Ala Ala Asn Gln Tyr Asn Gly Arg Ala Glu Leu Pro His Ser 
545                 550                 555                 560 


Val Phe His Gly His Lys Gln Leu Ala Thr Lys Ile Arg Leu Trp His 
                565                 570                 575     


Gln Gln Gly Glu Arg Cys Leu Tyr Thr Gly Lys Thr Ile Ser Ile His 
            580                 585                 590         


Asp Leu Ile Asn Asn Pro Asn Gln Phe Glu Ile Asp His Ile Leu Pro 
        595                 600                 605             


Leu Ser Ile Thr Phe Asp Asp Ser Leu Ala Asn Lys Val Leu Val Tyr 
    610                 615                 620                 


Ala Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu 
625                 630                 635                 640 


Asp Ser Met Asp Asp Ala Trp Ser Phe Arg Glu Leu Lys Ala Phe Val 
                645                 650                 655     


Arg Glu Ser Lys Ser Leu Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr 
            660                 665                 670         


Glu Glu Asp Ile Ser Arg Phe Asp Val Arg Lys Lys Phe Ile Glu Arg 
        675                 680                 685             


Asn Leu Val Asp Thr Arg Tyr Ala Ser Arg Val Val Leu Asn Ala Leu 
    690                 695                 700                 


Gln Glu His Phe Arg Ala His Lys Thr Asp Thr Lys Val Ser Val Val 
705                 710                 715                 720 


Arg Gly Gln Phe Thr Ser Gln Leu Arg Arg His Trp Gly Ile Glu Lys 
                725                 730                 735     


Thr Arg Asp Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala 
            740                 745                 750         


Ala Ser Ser Gln Leu Asn Leu Trp Lys Lys Gln Lys Asn Thr Leu Val 
        755                 760                 765             


Asn Tyr Ser Glu Asn Gln Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile 
    770                 775                 780                 


Ser Asp Asp Glu Tyr Lys Glu Ser Val Phe Lys Ala Pro Tyr Gln His 
785                 790                 795                 800 


Phe Val Asp Thr Leu Lys Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe 
                805                 810                 815     


Ser Tyr Gln Val Asp Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr 
            820                 825                 830         


Ile Tyr Ala Thr Arg Gln Ala Lys Val Gly Lys Asp Lys Lys Asp Glu 
        835                 840                 845             


Thr Tyr Val Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Thr Gly Tyr 
    850                 855                 860                 


Asp Ala Phe Ile Lys Ile Tyr Gln Lys Asp Lys Ser Lys Phe Leu Met 
865                 870                 875                 880 


Tyr Arg His Asp Pro Gln Thr Phe Glu Lys Val Ile Glu Pro Ile Leu 
                885                 890                 895     


Glu Asn Tyr Pro Asn Lys Glu Met Asn Glu Lys Gly Lys Glu Val Pro 
            900                 905                 910         


Cys Asn Pro Phe Leu Lys Tyr Lys Glu Glu His Gly Asp Tyr Ile Arg 
        915                 920                 925             


Lys Tyr Ser Lys Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr 
    930                 935                 940                 


Tyr Asp Ser Lys Leu Gly Asn His Ile Asp Ile Thr Pro Lys Asp Ser 
945                 950                 955                 960 


Asn Asn Lys Val Val Leu Gln Ser Val Ser Pro Trp Arg Ala Asp Val 
                965                 970                 975     


Tyr Phe Asn Lys Thr Thr Gly Lys Tyr Glu Ile Leu Gly Leu Lys Tyr 
            980                 985                 990         


Ala Asp Leu Gln Phe Glu Lys Gly  Thr Gly Thr Tyr Lys  Ile Ser Gln 
        995                 1000                 1005             


Glu Lys  Tyr Asn Val Ile Lys  Lys Lys Glu Gly Val  Asp Ser Asp 
    1010                 1015                 1020             


Ser Glu  Phe Lys Phe Thr Leu  Tyr Lys Asn Asp Leu  Leu Leu Ile 
    1025                 1030                 1035             


Lys Asp  Thr Glu Thr Lys Glu  Gln Gln Leu Phe Arg  Phe Leu Ser 
    1040                 1045                 1050             


Arg Thr  Lys Pro Asn Val Lys  His Tyr Val Glu Leu  Lys Pro Tyr 
    1055                 1060                 1065             


Asp Lys  Gln Lys Phe Glu Gly  Asn Glu Ser Leu Ile  Asn Val Leu 
    1070                 1075                 1080             


Gly Ala  Val Ala Lys Gly Gly  Gln Cys Gln Lys Gly  Ile Asn Lys 
    1085                 1090                 1095             


Pro Asn  Ile Ser Ile Tyr Lys  Val Arg Thr Asp Val  Leu Gly Asn 
    1100                 1105                 1110             


Gln His  Ile Ile Lys Asn Glu  Gly Asp Lys Pro Lys  Leu Asp Phe 
    1115                 1120                 1125             


<210>  699
<211>  1128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  699

Met Asp Glu Lys Met Asp Tyr Arg Ile Gly Leu Asp Ile Gly Ile Ala 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Leu Gln Asn Asn Ser Asp Asp Glu Pro Val 
            20                  25                  30          


Arg Ile Val Asp Leu Gly Val Arg Ile Phe Asp Thr Ala Glu Ile Pro 
        35                  40                  45              


Lys Thr Gly Glu Ser Leu Ala Gly Pro Arg Arg Ala Ala Arg Thr Thr 
    50                  55                  60                  


Arg Arg Arg Leu Arg Arg Arg Lys His Arg Leu Asp Arg Ile Lys Trp 
65                  70                  75                  80  


Leu Phe Glu Asn Gln Gly Leu Ile Asn Ile Asp Asp Phe Leu Lys Arg 
                85                  90                  95      


Tyr Asn Met Ala Gly Leu Pro Asp Val Tyr Gln Leu Arg Tyr Glu Ala 
            100                 105                 110         


Leu Asp Arg Lys Leu Thr Asp Glu Glu Leu Ala Gln Val Leu Leu His 
        115                 120                 125             


Ile Ala Lys His Arg Gly Phe Arg Ser Thr Arg Lys Ala Glu Thr Ala 
    130                 135                 140                 


Ala Lys Glu Asn Gly Ala Val Leu Lys Ala Thr Asp Glu Asn Gln Lys 
145                 150                 155                 160 


Arg Met Gln Glu Lys Gly Tyr Arg Thr Val Gly Glu Met Ile Tyr Leu 
                165                 170                 175     


Asp Glu Ala Phe Arg Thr Gly Cys Ser Trp Ser Glu Lys Gly Tyr Ile 
            180                 185                 190         


Leu Thr Pro Arg Asn Lys Ala Glu Asn Tyr Gln His Thr Met Leu Arg 
        195                 200                 205             


Ala Met Leu Val Glu Glu Val Lys Glu Ile Phe Ser Ser Gln Arg Arg 
    210                 215                 220                 


Leu Gly Asn Glu Lys Ala Thr Glu Glu Leu Glu Glu Lys Tyr Leu Glu 
225                 230                 235                 240 


Ile Met Thr Ser Gln Arg Ser Phe Asp Leu Gly Pro Gly Met Gln Pro 
                245                 250                 255     


Asp Gly Lys Pro Ser Pro Tyr Ala Met Glu Gly Phe Ser Asp Arg Val 
            260                 265                 270         


Gly Lys Cys Thr Phe Leu Gly Asp Gln Gly Glu Leu Arg Gly Ala Lys 
        275                 280                 285             


Gly Thr Tyr Thr Ala Glu Tyr Phe Val Ala Leu Gln Lys Ile Asn His 
    290                 295                 300                 


Thr Lys Leu Val Asn Gln Asp Gly Glu Thr Arg Asn Phe Thr Glu Glu 
305                 310                 315                 320 


Glu Arg Arg Ala Leu Thr Leu Leu Leu Phe Thr Gln Lys Glu Val Lys 
                325                 330                 335     


Tyr Ala Ala Val Arg Lys Lys Leu Gly Leu Pro Glu Asp Ile Leu Phe 
            340                 345                 350         


Tyr Asn Leu Asn Tyr Lys Lys Ala Ala Thr Lys Glu Glu Gln Gln Lys 
        355                 360                 365             


Glu Asn Gln Asn Thr Glu Lys Ala Lys Phe Ile Gly Met Pro Tyr Tyr 
    370                 375                 380                 


His Asp Tyr Lys Lys Cys Leu Glu Glu Arg Val Lys Tyr Leu Thr Glu 
385                 390                 395                 400 


Asn Glu Val Arg Asp Leu Phe Asp Glu Ile Gly Thr Ile Leu Thr Cys 
                405                 410                 415     


Tyr Lys Asn Asp Asp Ser Arg Thr Glu Arg Leu Ala Lys Leu Gly Leu 
            420                 425                 430         


Val Pro Ile Glu Met Glu Gly Leu Leu Ala Tyr Thr Pro Thr Lys Phe 
        435                 440                 445             


Gln His Leu Ser Met Lys Ala Met Arg Asn Ile Ile Pro Phe Leu Glu 
    450                 455                 460                 


Lys Gly Met Thr Tyr Asp Lys Ala Cys Glu Glu Ala Gly Tyr Asp Phe 
465                 470                 475                 480 


Lys Ala Asp Ser Lys Gly Ile Lys Gln Lys Leu Leu Thr Gly Glu Lys 
                485                 490                 495     


Val Asn Gln Thr Ile Asn Glu Ile Thr Asn Pro Val Val Lys Arg Ser 
            500                 505                 510         


Val Ser Gln Thr Val Lys Val Ile Asn Ala Ile Ile Arg Thr Tyr Gly 
        515                 520                 525             


Ser Pro Gln Ala Ile Asn Ile Glu Leu Ala Arg Glu Met Ser Lys Thr 
    530                 535                 540                 


Phe Glu Glu Arg Arg Lys Ile Lys Gly Asp Met Glu Lys Arg Gln Lys 
545                 550                 555                 560 


Asn Asn Glu Asp Val Lys Lys Gln Ile Gln Glu Leu Gly Lys Leu Ser 
                565                 570                 575     


Pro Thr Gly Gln Asp Ile Leu Lys Tyr Arg Leu Trp Gln Glu Gln Gln 
            580                 585                 590         


Gly Ile Cys Met Tyr Ser Gly Lys Thr Ile Pro Leu Glu Glu Leu Phe 
        595                 600                 605             


Lys Pro Gly Tyr Asp Ile Asp His Ile Leu Pro Tyr Ser Ile Thr Phe 
    610                 615                 620                 


Asp Asp Ser Phe Arg Asn Lys Val Leu Val Thr Ser Gln Glu Asn Arg 
625                 630                 635                 640 


Gln Lys Gly Asn Arg Thr Pro Tyr Glu Tyr Met Gly Asn Asp Glu Gln 
                645                 650                 655     


Arg Trp Asn Glu Phe Glu Thr Arg Val Lys Thr Thr Ile Arg Asp Tyr 
            660                 665                 670         


Lys Lys Gln Gln Lys Leu Leu Lys Lys His Phe Ser Glu Glu Glu Arg 
        675                 680                 685             


Ser Glu Phe Lys Glu Arg Asn Leu Thr Asp Thr Lys Tyr Ile Thr Thr 
    690                 695                 700                 


Val Ile Tyr Asn Met Ile Arg Gln Asn Leu Glu Met Ala Pro Leu Asn 
705                 710                 715                 720 


Arg Pro Glu Lys Lys Lys Gln Val Arg Ala Val Asn Gly Ala Ile Thr 
                725                 730                 735     


Ala Tyr Leu Arg Lys Arg Trp Gly Leu Pro Gln Lys Asn Arg Glu Thr 
            740                 745                 750         


Asp Thr His His Ala Met Asp Ala Val Val Ile Ala Cys Cys Thr Asp 
        755                 760                 765             


Gly Met Ile Gln Lys Ile Ser Arg Tyr Thr Lys Val Arg Glu Arg Cys 
    770                 775                 780                 


Tyr Ser Lys Gly Thr Glu Phe Val Asp Ala Glu Thr Gly Glu Ile Phe 
785                 790                 795                 800 


Arg Pro Glu Asp Tyr Ser Arg Ala Glu Trp Asp Glu Ile Phe Gly Val 
                805                 810                 815     


His Ile Pro Lys Pro Trp Glu Thr Phe Arg Ala Glu Leu Asp Val Arg 
            820                 825                 830         


Met Gly Asp Asp Pro Lys Gly Phe Leu Asp Thr His Ser Asp Val Ala 
        835                 840                 845             


Leu Glu Leu Asp Tyr Pro Glu Tyr Ile Tyr Glu Asn Leu Arg Pro Ile 
    850                 855                 860                 


Phe Val Ser Arg Met Pro Asn His Lys Val Thr Gly Ala Ala His Ala 
865                 870                 875                 880 


Asp Thr Ile Arg Ser Pro Arg His Phe Lys Asp Glu Gly Ile Val Leu 
                885                 890                 895     


Thr Lys Thr Ala Leu Thr Asp Leu Lys Leu Asp Lys Asp Gly Glu Ile 
            900                 905                 910         


Asp Gly Tyr Tyr Asn Pro Gln Ser Asp Leu Leu Leu Tyr Glu Ala Leu 
        915                 920                 925             


Lys Lys Gln Leu Leu Leu Tyr Gly Asn Asp Ala Lys Lys Ala Phe Ala 
    930                 935                 940                 


Gln Asp Phe His Lys Pro Lys Ala Asp Gly Thr Glu Gly Pro Val Val 
945                 950                 955                 960 


Arg Lys Val Lys Ile Gln Lys Lys Gln Thr Met Gly Val Phe Val Asp 
                965                 970                 975     


Ser Gly Asn Gly Ile Ala Glu Asn Gly Gly Met Val Arg Ile Asp Val 
            980                 985                 990         


Phe His Val Asn Gly Lys Tyr Tyr  Phe Val Pro Val Tyr  Thr Ala Asp 
        995                 1000                 1005             


Val Val  Lys Lys Val Leu Pro  Asn Arg Ala Ala Thr  Ala His Lys 
    1010                 1015                 1020             


Pro Tyr  Gly Glu Trp Lys Val  Met Glu Asp Lys Asp  Phe Leu Phe 
    1025                 1030                 1035             


Ser Leu  Tyr Ser Arg Asp Leu  Ile His Ile Lys Ser  Lys Lys Asp 
    1040                 1045                 1050             


Ile Pro  Ile Lys Met Val Asn  Gly Gly Met Glu Gly  Ile Lys Glu 
    1055                 1060                 1065             


Thr Tyr  Ala Tyr Tyr Ile Gly  Ala Asp Ile Ser Ala  Ala Asn Ile 
    1070                 1075                 1080             


Gln Gly  Ile Ala His Asp Ser  Arg Tyr Lys Phe Arg  Gly Leu Gly 
    1085                 1090                 1095             


Ile Gln  Ser Leu Asp Val Leu  Glu Lys Cys Gln Ile  Asp Val Leu 
    1100                 1105                 1110             


Gly His  Val Ser Val Val Arg  Ser Glu Lys Arg Met  Gly Phe Ser 
    1115                 1120                 1125             


<210>  700
<211>  1128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  700

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Val His Lys Asn Ala 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Val Glu Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn His Ile Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Ile Glu Glu Asp Gly Lys 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ala Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Ser Tyr Leu Gln Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Thr Thr Lys Lys Asp Ser Glu Asp Glu Tyr Ile Thr Leu Asp Asn 
                245                 250                 255     


Ile Phe Gly Ile Leu Ile Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr 
            260                 265                 270         


Arg Ala Ala Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Glu 
    290                 295                 300                 


Glu Gln Lys Asn Gln Ile Ile Thr Tyr Val Lys Asn Glu Lys Ala Met 
305                 310                 315                 320 


Gly Pro Ala Lys Leu Phe Lys Tyr Ile Ala Lys Leu Leu Ala Cys Asp 
                325                 330                 335     


Val Ala Asp Ile Lys Gly Tyr Arg Ile Asp Lys Ser Asp Lys Pro Glu 
            340                 345                 350         


Ile His Thr Phe Glu Ala Tyr Arg Lys Met Lys Thr Leu Glu Thr Ile 
        355                 360                 365             


Asp Val Glu Gln Met Glu Arg Glu Lys Leu Asp Lys Leu Ala Tyr Val 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Arg Glu Gly Ile Gln Glu Ala Ile Glu His 
385                 390                 395                 400 


Glu Phe Ser Asp Gly Thr Phe Ser Gln Glu Gln Val Asp Glu Leu Val 
                405                 410                 415     


Gln Phe Arg Lys Ser Asn Ser Ser Ile Phe Gly Lys Gly Trp His Asn 
            420                 425                 430         


Phe Ser Val Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Ala Thr 
        435                 440                 445             


Ser Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Arg 
    450                 455                 460                 


Thr Ser Ser Ser Asn Lys Thr Lys Tyr Ile Asp Glu Lys Arg Leu Thr 
465                 470                 475                 480 


Glu Glu Ile Tyr Asn Pro Val Val Ala Lys Ser Val Arg Gln Ala Ile 
                485                 490                 495     


Lys Ile Val Asn Ala Ala Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile 
            500                 505                 510         


Val Ile Glu Met Ala Arg Glu Met Asn Glu Asp Asp Glu Lys Lys Ala 
        515                 520                 525             


Ile Lys Lys Ile Gln Glu Ala Asn Lys Ser Glu Lys Asp Ala Ala Met 
    530                 535                 540                 


Arg Lys Ala Ala Asn Gln Tyr Asn Gly Lys Ala Glu Leu Pro His Ser 
545                 550                 555                 560 


Val Phe His Gly His Lys Gln Leu Ala Thr Lys Ile Arg Leu Trp His 
                565                 570                 575     


Gln Gln Gly Glu Arg Cys Leu Tyr Thr Gly Lys Thr Ile Ser Ile His 
            580                 585                 590         


Asp Leu Ile Asn Asn Pro Asn Gln Phe Glu Ile Asp His Ile Leu Pro 
        595                 600                 605             


Leu Ser Ile Thr Phe Asp Asp Ser Leu Ala Asn Lys Val Leu Val Tyr 
    610                 615                 620                 


Ala Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu 
625                 630                 635                 640 


Asp Ser Met Asp Asp Ala Trp Ser Phe Arg Glu Leu Lys Ala Phe Val 
                645                 650                 655     


Arg Glu Ser Lys Thr Leu Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr 
            660                 665                 670         


Glu Glu Asp Ile Ser Lys Phe Asp Val Arg Lys Lys Phe Ile Glu Arg 
        675                 680                 685             


Asn Leu Val Asp Thr Arg Tyr Ala Ser Arg Val Val Leu Asn Ala Leu 
    690                 695                 700                 


Gln Glu His Phe Arg Ala Arg Lys Ile Asp Thr Lys Val Ser Val Val 
705                 710                 715                 720 


Arg Gly Gln Phe Thr Ser Gln Leu Arg Arg His Trp Gly Ile Asp Lys 
                725                 730                 735     


Thr Arg Asp Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala 
            740                 745                 750         


Ala Ser Ser Gln Leu Asn Leu Trp Lys Lys Gln Lys Asn Thr Leu Val 
        755                 760                 765             


Ser Tyr Ser Glu Asp Gln Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile 
    770                 775                 780                 


Ser Asp Asp Glu Tyr Lys Glu Ser Val Phe Lys Ala Pro Tyr Gln His 
785                 790                 795                 800 


Phe Val Asp Thr Leu Lys Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe 
                805                 810                 815     


Ser Tyr Gln Val Asp Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr 
            820                 825                 830         


Ile Tyr Ala Thr Arg Gln Ala Lys Val Gly Lys Asp Lys Lys Asp Glu 
        835                 840                 845             


Thr Tyr Val Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Ala Gly Tyr 
    850                 855                 860                 


Asp Ala Phe Ile Lys Ile Tyr Gln Lys Asp Lys Ser Lys Phe Leu Met 
865                 870                 875                 880 


Tyr Arg His Asp Pro Gln Thr Phe Glu Lys Val Ile Glu Pro Ile Leu 
                885                 890                 895     


Glu Asn Tyr Pro Asn Lys Glu Met Asn Glu Lys Gly Lys Glu Val Pro 
            900                 905                 910         


Cys Asn Pro Phe Leu Lys Tyr Lys Glu Glu His Gly Asp Tyr Ile Arg 
        915                 920                 925             


Lys Tyr Ser Lys Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr 
    930                 935                 940                 


Tyr Asp Ser Lys Leu Gly Asn His Ile Asp Ile Thr Pro Lys Asn Ser 
945                 950                 955                 960 


Asn Asn Lys Val Val Leu Gln Ser Val Ser Pro Trp Arg Ala Asp Val 
                965                 970                 975     


Tyr Phe Asn Lys Thr Thr Gly Lys Tyr Glu Ile Leu Gly Leu Lys Tyr 
            980                 985                 990         


Ala Asp Leu Gln Phe Glu Lys Gly  Thr Gly Thr Tyr Lys  Ile Ser Gln 
        995                 1000                 1005             


Glu Lys  Tyr Asn Asp Ile Lys  Lys Lys Glu Gly Val  Asp Ser Asp 
    1010                 1015                 1020             


Ser Glu  Phe Lys Phe Thr Leu  Tyr Lys Asn Asp Leu  Leu Leu Ile 
    1025                 1030                 1035             


Lys Asp  Thr Glu Thr Lys Glu  Gln Gln Leu Phe Arg  Phe Leu Ser 
    1040                 1045                 1050             


Arg Ser  Met Pro Asn Lys Lys  His Tyr Val Glu Leu  Lys Pro Tyr 
    1055                 1060                 1065             


Ser Lys  Asp Lys Phe Glu Lys  Asn Glu Thr Leu Ile  Glu Ile Leu 
    1070                 1075                 1080             


Gly Pro  Thr Asp Lys Ser Gly  Arg Cys Ile Lys Gly  Leu Ala Lys 
    1085                 1090                 1095             


Ser Asn  Leu Ser Ile Phe Lys  Val Arg Thr Asp Ile  Leu Gly Asn 
    1100                 1105                 1110             


Gln His  Ile Ile Lys Asn Glu  Gly Asp Lys Pro Lys  Leu Asp Phe 
    1115                 1120                 1125             


<210>  701
<211>  1128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  701

Met Thr Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asp Glu Lys Lys Glu Lys Ile Leu Gly Met Gly Ser Arg 
            20                  25                  30          


Ile Phe Pro Glu Gly Val Val Asn Ile Gly Glu Gly Glu Gly Arg Glu 
        35                  40                  45              


Thr Ser Lys Asn Ala Ser Arg Thr Asp Ala Arg Gly Thr Arg Arg Gln 
    50                  55                  60                  


Phe Phe Arg Arg Arg Leu Arg Lys Arg Tyr Leu Leu Arg Glu Leu Ala 
65                  70                  75                  80  


Lys Lys Gly Leu Cys Pro Ile Asn Tyr Thr Leu Val Lys Glu Trp Asn 
                85                  90                  95      


Glu Ala Gln Ile Phe Lys Asn Lys Gln Leu Gln Glu Trp Phe Lys Leu 
            100                 105                 110         


Asn Pro Tyr Glu Leu Arg Ser Lys Ala Ile Gln Glu Lys Ile Ser Leu 
        115                 120                 125             


Glu Glu Leu Gly Arg Ile Phe Tyr His Phe Ile Gln Arg Arg Gly Phe 
    130                 135                 140                 


Gln Ser Asn Ser Arg Ser Ala Gly Val Asp Asn Ile Glu Lys Ser Val 
145                 150                 155                 160 


Ile Phe Lys Gly Asp Thr Lys Thr Gly Lys Ile Gly Ile Ser Glu Thr 
                165                 170                 175     


Leu Glu Ser Ile Lys Asn Thr Lys Thr Leu Gly Ser Tyr Leu His Thr 
            180                 185                 190         


Ile Tyr Pro Lys Gln Asn Lys Pro Tyr Ser Gly His Lys Glu Arg Ile 
        195                 200                 205             


Arg Asn Arg Tyr Thr Thr Arg Gln Met Tyr Ile Tyr Glu Phe Glu Thr 
    210                 215                 220                 


Ile Trp Glu Tyr Gln Lys Gln Phe Tyr Ser Glu Leu Ser Asn Glu Leu 
225                 230                 235                 240 


Lys Thr Val Leu Gly Gly Arg Lys Lys Asp Gly Tyr Lys Glu Asp Gly 
                245                 250                 255     


Ile Leu Phe His Gln Arg Pro Leu Arg Ser Gln Lys His Leu Val Gly 
            260                 265                 270         


Tyr Cys Ser Phe Glu Pro Lys Lys Thr Lys Cys Gln Ile Asn Thr Ile 
        275                 280                 285             


Pro Phe Glu Leu Phe Arg Ile Tyr Gln Trp Val Asn Thr Val Asp Cys 
    290                 295                 300                 


Asn Glu Lys Val Thr Ser Glu Asp Arg Gly Lys Met Val Glu Leu Leu 
305                 310                 315                 320 


Leu Ser Lys Glu Lys Val Ser Phe Lys Glu Ile Arg Lys Val Ile Asn 
                325                 330                 335     


Lys Ser Asp Gly Tyr Tyr Gln Phe Asn Tyr Lys Asn Asp Asp Lys Ile 
            340                 345                 350         


Val Gly Ser Tyr Thr Ile Ser Asn Leu Ser Asn Lys Lys Phe Phe Gly 
        355                 360                 365             


Lys Lys Trp Phe Asp Phe Ser Asp Lys Glu Gln Glu Asp Ile Trp His 
    370                 375                 380                 


Val Leu Ser Phe Phe Asp Asp Arg Asp Lys Leu Lys Gln Tyr Ala Ile 
385                 390                 395                 400 


Lys Tyr Trp Gly Phe Asn Glu Glu Lys Ala Val Lys Ile Ser Lys Phe 
                405                 410                 415     


Asn Leu Lys Glu Gly Tyr Ser Asn Leu Ser Arg Lys Ala Ile His Asn 
            420                 425                 430         


Ile Leu Pro Phe Leu Lys Leu Gly Phe Met Tyr Asp Val Ala Val Gly 
        435                 440                 445             


Leu Gly Gly Val Lys Asn Ala Leu Ala Asp Lys Trp Ile Glu Asn Glu 
    450                 455                 460                 


Gln Phe Ile Leu Asp Asn Val Pro Ser Val Val Arg Ser Asn Phe Lys 
465                 470                 475                 480 


Gly Gly Tyr Ile Glu Pro Leu Lys Gln Met Leu Lys Tyr Glu Phe Asp 
                485                 490                 495     


Val Ser Asp Lys Gln Leu Leu Lys Leu Tyr His His Ser Ser Ala Ile 
            500                 505                 510         


Glu Thr Thr Glu Leu Leu Lys Lys Leu Pro Ile Asn Ser Glu Ala Asp 
        515                 520                 525             


Lys Glu Ile Gln Ser Ile Lys Asn Pro Val Val Ile Thr Ala Leu Phe 
    530                 535                 540                 


Glu Ile Arg Lys Leu Val Asn Glu Ile Ile Asp Thr Tyr Gly Lys Phe 
545                 550                 555                 560 


Asp Glu Ile Lys Val Glu Leu Ala Arg Asp Leu Lys Ala Ser Lys Ser 
                565                 570                 575     


Lys Arg Tyr Asp Thr Arg Arg Glu Gln Leu Arg Leu Glu Lys Glu Asn 
            580                 585                 590         


Asp Arg Ile Lys Ile Glu Leu Glu Asn Leu Gly Gln Arg Ile Asn His 
        595                 600                 605             


Ile Thr Ile Leu Lys Tyr Lys Leu Trp Glu Glu Cys Asp Lys Arg Cys 
    610                 615                 620                 


Pro Phe Thr Gly Lys Asn Ile Glu Val Asn Glu Leu Phe Ser Gly Glu 
625                 630                 635                 640 


Val Gln Ile Glu His Ile Phe Pro Trp Ser Arg Ser Leu Asn Asp Ser 
                645                 650                 655     


Phe Met Asn Lys Thr Leu Cys Phe Ala Asp Glu Asn Arg Ala Lys Gly 
            660                 665                 670         


Asn Lys Thr Pro Tyr Glu Phe Tyr Ser Glu Gln Gly Glu Gln Lys Trp 
        675                 680                 685             


Glu Glu Ile Lys Ile Gln Ala Leu Ser Cys Phe Lys Asn Lys Pro Asn 
    690                 695                 700                 


Tyr Pro Asn Ala Tyr Gln Lys Phe Lys Gln Phe Val Lys Lys Lys His 
705                 710                 715                 720 


Asp Glu Asp Phe Ile Ser Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser 
                725                 730                 735     


Lys Glu Ala Lys Asn Tyr Leu Ser Lys Ile Cys Asn Asn Val Gln Val 
            740                 745                 750         


Ala Pro Gly Gln Met Thr Ala Thr Leu Arg His Lys Trp Gly Leu Asn 
        755                 760                 765             


Thr Ile Leu Asn Lys Asp Asp Asp Thr Lys Thr Arg Asp Asp His Arg 
    770                 775                 780                 


His His Ala Ile Asp Ala Leu Val Met Ala Cys Ser Thr Arg Ser His 
785                 790                 795                 800 


Leu Gln Glu Leu Ser Lys Arg Asn Arg Tyr Gln Lys Asp Tyr Glu Phe 
                805                 810                 815     


Lys Asp Phe Pro Met Pro Trp Gln Phe Phe Arg Gln Asp Ala Glu Lys 
            820                 825                 830         


Ser Val Glu Gln Ile Leu Val Ser His Lys Lys Leu Asn Thr Ile Leu 
        835                 840                 845             


Thr Val Arg Thr His Lys Thr Lys Lys Asn Gly Val Val Tyr Lys Asn 
    850                 855                 860                 


Ile Gly Val Ala Ala Arg Gly Gln Leu His Lys Glu Thr Val Phe Gly 
865                 870                 875                 880 


Lys Arg Thr Ala Pro Asp Gly Ile Glu Ala Tyr His Val Arg Lys Pro 
                885                 890                 895     


Ile Glu Ser Leu Ser Thr His Lys His Ile Asn Lys Val Val Asp Glu 
            900                 905                 910         


Thr Ile Arg Leu Leu Ile Leu Lys Gln Val Asn Lys Leu Gly Gly Phe 
        915                 920                 925             


Val Lys Gly Lys Ile Pro Glu Gly Thr Phe Tyr Asn Thr Asp Glu Asn 
    930                 935                 940                 


Gly Asn Lys Gln Pro Gln Ile Phe Leu Pro Asn Lys Asn Gly Asn Pro 
945                 950                 955                 960 


Val Pro Ile Lys Lys Ile Arg Leu Arg Glu Asn Ile Gly Gly Ala Glu 
                965                 970                 975     


Gln Leu Lys Ser Thr Val Asn Gln His Val Asn Pro Arg Ser Asn His 
            980                 985                 990         


His Val Leu Ile Tyr Lys Asp Phe  Asp Asp Asn Leu Lys  Glu Lys Val 
        995                 1000                 1005             


Val Thr  Phe Trp Thr Ala Val  Glu Arg Lys Lys Gln  Lys Gln Ala 
    1010                 1015                 1020             


Val Phe  Gln Leu Pro Glu Asn  Gly Lys Glu Ile Val  Thr Thr Leu 
    1025                 1030                 1035             


Gln Ile  Asn Asp Met Phe Leu  Leu Gly Leu Lys Asp  Asp Glu Ile 
    1040                 1045                 1050             


Asn Trp  Glu His Pro Lys Phe  Thr Leu Leu Lys Glu  His Leu Tyr 
    1055                 1060                 1065             


Arg Val  Gln Lys Phe Thr Ser  Gly Asp Tyr Tyr Phe  Arg Lys His 
    1070                 1075                 1080             


Ile Glu  Ser Asn Leu Asn Gly  Glu Leu Gly Lys Ala  Tyr Gln Tyr 
    1085                 1090                 1095             


Ile Lys  Gly Phe Gly Glu Gly  Lys Thr Gly Trp Lys  Thr Phe Asn 
    1100                 1105                 1110             


Pro Ile  Lys Ile Lys Val Ser  Val Ser Gly Lys Ile  Glu Lys Val 
    1115                 1120                 1125             


<210>  702
<211>  1129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  702

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Ala Gly Ile Leu Lys Lys Asp Thr Gly Glu Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Leu Arg Arg Ser 
        35                  40                  45              


Ser Arg Gln Ala Arg Arg Ser Thr Arg Arg Lys Arg His Arg Ala Val 
    50                  55                  60                  


Arg Leu His Asp Leu Phe Glu Asp Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Lys Leu Arg Val Glu Gly Met 
                85                  90                  95      


Asp Ile Gln Leu Thr Thr Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Ile Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Val Thr Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ser Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Glu Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Ser Thr Ser Thr Tyr Arg Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Lys Ile Thr 
        195                 200                 205             


Asp Asp Phe Ile Asp Asp Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Lys Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Thr Thr Lys Lys Asp Ser Glu Gly Lys Tyr Ile Thr Leu Asp Asn 
                245                 250                 255     


Ile Phe Gly Val Leu Ile Gly Lys Cys Thr Phe Tyr Pro Asp Glu Tyr 
            260                 265                 270         


Arg Ala Ser Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Glu 
    290                 295                 300                 


Lys Gln Lys Lys Thr Ile Val Glu Tyr Ala Lys Thr Ala Lys Thr Leu 
305                 310                 315                 320 


Gly Ala Ser Thr Leu Leu Lys Tyr Ile Ala Lys Met Val Asp Ala Ser 
                325                 330                 335     


Val Asp Gln Ile His Gly Tyr Arg Val Asp Val Asn Lys Lys Pro Glu 
            340                 345                 350         


Met His Thr Phe Asp Val Tyr Arg Lys Met Gln Ser Leu Glu Thr Phe 
        355                 360                 365             


Asn Val Asn Glu Met Pro Arg Glu Val Leu Asp Glu Leu Ala His Ile 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr 
385                 390                 395                 400 


Lys Leu Lys Gly Thr Phe Asn Gln Asp Gln Val Leu Glu Leu Val Gln 
                405                 410                 415     


Phe Arg Lys Asn Asn Ser Ser Leu Phe Ser Lys Gly Trp His Asn Phe 
            420                 425                 430         


Ser Leu Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Ala Thr Ser 
        435                 440                 445             


Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys 
    450                 455                 460                 


Glu Ile Ser Lys Arg Thr Lys Tyr Ile Asp Glu Lys Glu Ile Thr Glu 
465                 470                 475                 480 


Glu Ile Tyr Asn Pro Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys 
                485                 490                 495     


Ile Ile Asn Glu Ala Thr Lys Gln Tyr Gly Ile Phe Asp His Ile Val 
            500                 505                 510         


Ile Glu Met Ala Arg Leu Thr Asn Glu Glu Asp Ala Lys Lys Asp Tyr 
        515                 520                 525             


Ile Lys Arg Gln Lys Ala Asn Leu Asp Glu Lys Asn Ala Ala Met Glu 
    530                 535                 540                 


Lys Ala Ala Phe Gln Tyr Asn Gly Lys Lys Glu Leu Pro Asp Ser Ile 
545                 550                 555                 560 


Phe His Gly His Lys Glu Leu Ala Thr Lys Ile Arg Leu Trp His Gln 
                565                 570                 575     


Gln Gly Glu Lys Cys Leu Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp 
            580                 585                 590         


Leu Ile Gln Asn Gln Tyr Lys Tyr Glu Ile Asp His Ile Leu Pro Leu 
        595                 600                 605             


Ser Leu Ser Phe Asp Asp Ser Leu Ser Asn Lys Val Leu Val Leu Ala 
    610                 615                 620                 


Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp 
625                 630                 635                 640 


Arg Met Asp Asp Ala Trp Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys 
                645                 650                 655     


Asp Ser Lys Leu Leu Gly Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu 
            660                 665                 670         


Glu Asp Ile Ser Lys Ile Glu Val Lys Gln Lys Phe Ile Glu Arg Asn 
        675                 680                 685             


Leu Val Asp Thr Arg Tyr Ser Ser Arg Val Val Leu Asn Ala Leu Gln 
    690                 695                 700                 


Asp Phe Tyr Lys Asn His His Phe Asp Thr Thr Ile Ser Val Val Arg 
705                 710                 715                 720 


Gly Gln Phe Thr Ser Gln Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser 
                725                 730                 735     


Arg Glu Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala Ala 
            740                 745                 750         


Ser Ser Gln Leu Arg Leu Trp Lys Lys Gln Asn Asn Pro Leu Ile Phe 
        755                 760                 765             


Tyr Lys Glu Gly Gln Phe Val Asp Ser Glu Thr Gly Glu Ile Leu Ser 
    770                 775                 780                 


Leu Asn Asp Asp Glu Tyr Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp 
785                 790                 795                 800 


His Phe Val Asp Thr Leu Arg Ser Lys Thr Phe Glu Asp Ser Ile Leu 
                805                 810                 815     


Phe Ser Tyr Gln Val Asp Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala 
            820                 825                 830         


Thr Ile Tyr Ala Thr Arg Lys Ala Lys Leu Asp Lys Asp Lys Ser Lys 
        835                 840                 845             


Glu Thr Tyr Val Leu Ser Lys Ile Lys Asp Ile Tyr Ser Gln Ala Gly 
    850                 855                 860                 


Tyr Asp Ala Phe Ile Lys Ile Tyr Asn Lys Asp Lys Ser Lys Phe Leu 
865                 870                 875                 880 


Met Tyr His Lys Asp Ser Gln Thr Phe Glu Lys Val Ile Glu Glu Ile 
                885                 890                 895     


Leu Arg Thr Tyr Pro Ser Lys Glu Phe Asn Asp Lys Asn Lys Glu Val 
            900                 905                 910         


Pro Cys Asn Pro Phe Glu Lys Tyr Arg Gln Glu Asn Gly Pro Ile Arg 
        915                 920                 925             


Lys Tyr Ser Lys Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu Lys Tyr 
    930                 935                 940                 


Tyr Asp Lys Asn Ile Pro Glu Ser Phe Ile Asn Ile Thr Pro Ser Asn 
945                 950                 955                 960 


Ser Lys Asn Lys Val Ile Leu Arg Gln Leu Asn Pro Trp Arg Thr Asp 
                965                 970                 975     


Val Tyr Phe Asn His Gln Thr Glu Lys Tyr Glu Leu Leu Gly Leu Lys 
            980                 985                 990         


Tyr Ala Asp Leu Gln Phe Glu Lys  Lys Thr Gly Thr Tyr  Ser Ile Thr 
        995                 1000                 1005             


Ile Asp  Lys Tyr Asn Glu Ile  Lys Glu Lys Glu Gly  Val Gly Glu 
    1010                 1015                 1020             


Gln Ser  Glu Phe Lys Phe Thr  Leu Tyr Lys Asn Asp  Leu Leu Leu 
    1025                 1030                 1035             


Ile Lys  Asp Thr Lys Asn Asn  Glu Lys Gln Ile Phe  Arg Phe Leu 
    1040                 1045                 1050             


Ser Arg  Thr Met Pro Asn Val  Lys His Tyr Val Glu  Leu Lys Pro 
    1055                 1060                 1065             


Tyr Asn  Lys Ala Lys Phe Asp  Gly Asn Gln Pro Leu  Ile Thr Ile 
    1070                 1075                 1080             


Phe Gly  Asn Val Ala Lys Gly  Gly Gln Cys Leu Lys  Gly Leu Asn 
    1085                 1090                 1095             


Lys Pro  Asn Ile Ser Ile Tyr  Lys Ile Lys Thr Asp  Val Leu Gly 
    1100                 1105                 1110             


Asn Lys  Tyr Phe Ile Lys Lys  Glu Gly Asp Lys Pro  Lys Leu Lys 
    1115                 1120                 1125             


Phe 
    


<210>  703
<211>  1129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  703

Met Ala Asp Arg Ile Ser Leu Gly Leu Asp Ile Gly Val Ala Ser Val 
1               5                   10                  15      


Gly Phe Ser Val Leu Asp Ile Asp Lys Gly Lys Val Ile Glu Leu Gly 
            20                  25                  30          


Ser Arg Leu Phe Asn Ala Thr Val Ala Glu Gly Asn Gln Asp Arg Arg 
        35                  40                  45              


Ser Met Arg Gly Ala Arg Arg Leu Leu Asn Arg Asn Lys Gln Arg Arg 
    50                  55                  60                  


Gln Asp Thr Gly Lys Leu Phe Gln Lys Phe Gly Leu Leu Asp Asn Phe 
65                  70                  75                  80  


Asn Lys Asp Asn Phe Tyr Asp Thr Phe Asn Gln Asn Trp Asn Pro Tyr 
                85                  90                  95      


Glu Leu Arg Val Lys Gly Leu Thr Glu His Leu Thr Lys Lys Glu Leu 
            100                 105                 110         


Ala Glu Ser Leu Tyr Gln Ile Val Lys His Arg Gly Ile Ser Tyr Ala 
        115                 120                 125             


Leu Lys Asp Ala Asp Ala Asp Glu Gly Gly Thr Asp Tyr Ser Ala Ser 
    130                 135                 140                 


Leu Lys Ile Asn Ser Gln Glu Leu Ala Glu Lys Thr Pro Ala Gln Ile 
145                 150                 155                 160 


Gln Leu Gln Arg Leu Asn Asp Tyr Gly Lys Val Arg Gly Gln Val Val 
                165                 170                 175     


Ile Gly Asp Asp Pro Asp Asn Gln Lys Val Leu Leu Asn Val Phe Pro 
            180                 185                 190         


Thr Ser Ala Tyr Glu Lys Glu Ala Lys Gln Ile Ile Ala Thr Gln Lys 
        195                 200                 205             


Gln Phe Tyr Pro Glu Ile Leu Thr Asp Glu Phe Thr Lys Glu Tyr Cys 
    210                 215                 220                 


Lys Ile Leu Thr Arg Lys Arg Asp Tyr Phe Val Gly Pro Gly Asn Glu 
225                 230                 235                 240 


Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Lys Thr Asp Gly Arg Thr Leu 
                245                 250                 255     


Asp Asn Leu Phe Glu Glu Leu Ile Gly His Asp Lys Ile Tyr Pro Asp 
            260                 265                 270         


Glu Leu Arg Ala Ser Ala Ala Ser Tyr Thr Ala Gln Leu Phe Asn Val 
        275                 280                 285             


Leu Asn Asp Leu Asn Asn Leu Arg Ile Leu Ser Tyr Glu Asp Gly Lys 
    290                 295                 300                 


Leu Thr Lys Glu Asp Lys Glu Lys Ile Ile Ala Glu Ile Lys Ser Asn 
305                 310                 315                 320 


Thr Thr Thr Ile Asn Met Leu Asn Val Ile Lys Lys Val Ala Gly Cys 
                325                 330                 335     


Ser Lys Glu Asp Ile Lys Gly Phe Arg Val Asn Glu Lys Asp Lys Pro 
            340                 345                 350         


Glu Ile Ser Ser Met Ala Val Tyr Arg Lys Val His Lys Asp Leu Leu 
        355                 360                 365             


Lys Val Gly Val Asp Ile Ser Asp Trp Pro Val Glu Phe Ile Asp Lys 
    370                 375                 380                 


Leu Ser Phe Ile Leu Thr Leu Asn Thr Glu Asn Gly Glu Ile Arg Lys 
385                 390                 395                 400 


Gln Leu Asn Asn Arg Leu Val Pro Glu Phe Asp Phe Leu Asn Asp Asp 
                405                 410                 415     


Leu Ile Gln Leu Ile Ile Asp Asn Lys Asp Ser Phe Glu Ile Lys Thr 
            420                 425                 430         


Asn Asn Lys Trp His Arg Phe Ser Leu Lys Thr Met Asn Lys Leu Met 
        435                 440                 445             


Pro Glu Met Leu Glu Arg Pro Val Glu Gln Met Thr Leu Leu Asn Glu 
    450                 455                 460                 


Met Gly Leu Val Lys Lys Asp Lys Lys Arg Phe Glu Asn Asn Lys Tyr 
465                 470                 475                 480 


Leu Pro Tyr Lys Glu Ile Ala Lys Asp Ile Phe Asn Pro Val Ala Ser 
                485                 490                 495     


Lys Ser Val Arg Glu Ala Leu Lys Ile Val Asn Ala Val Leu Lys Lys 
            500                 505                 510         


Tyr Gly His Ile Asp Tyr Leu Val Ile Glu Met Pro Arg Asp Lys Asn 
        515                 520                 525             


Leu Lys Glu Glu Gln Asp Asn Ile Lys Glu Phe Gln Asn Lys Asn Lys 
    530                 535                 540                 


Lys Ala Lys Asp Ala Ala Phe Glu Ala Phe Val Lys Ser Val Gly Ser 
545                 550                 555                 560 


Glu Gln Arg Val Lys Glu Ala Leu Ser Lys Asn Arg Lys Leu Gln Met 
                565                 570                 575     


Lys Met Arg Leu Trp Tyr Gln Gln Gln Glu Ile Asp Pro Tyr Asn Gly 
            580                 585                 590         


Glu Thr Ile Asp Ala Thr Asp Leu Ile Asn Asn Pro Asp Lys Phe Glu 
        595                 600                 605             


Ile Asp His Ile Ile Pro Gln Ser Ile Ser Phe Asp Asp Ser Ile Asn 
    610                 615                 620                 


Asn Lys Thr Leu Cys Phe Ala Ser Met Asn Gln Val Lys Gly Gln Lys 
625                 630                 635                 640 


Thr Pro Tyr Glu Phe Met Leu Glu Gly His Gly Gln Gly Tyr Asp Lys 
                645                 650                 655     


Phe Lys Ala Thr Val Leu Asp Asn Lys Asn Phe Asp Arg Asn Lys Arg 
            660                 665                 670         


Lys Asn Tyr Leu Phe Glu Glu Asn Val Ser Asp Ile Glu Thr Arg Lys 
        675                 680                 685             


Arg Phe Leu Ser Arg Asn Leu Val Asp Thr Arg Tyr Ser Ser Arg Val 
    690                 695                 700                 


Val Leu Asn Ser Leu Gln Asp Phe Phe His Glu Lys Ser Ala Asp Thr 
705                 710                 715                 720 


Lys Val Thr Val Ile Arg Gly Lys Phe Thr Ser Asn Met Arg Lys His 
                725                 730                 735     


Trp His Ile Asp Lys Thr Arg Glu Thr Phe His His His Ala Ile Asp 
            740                 745                 750         


Ala Ser Ile Ile Ala Ala Thr Pro Phe Leu Arg Met Trp Lys Lys Gly 
        755                 760                 765             


Gly Thr Ile Phe Pro Val Lys Val Gly Glu Glu Ser Ile Asp Ile Glu 
    770                 775                 780                 


Thr Gly Glu Ile Leu Asp Asp Lys Lys Phe Asp Lys Val Met Tyr Glu 
785                 790                 795                 800 


Glu Pro Tyr Asn Gly Phe Val Ser Glu Ile Met Asn Ala Asp Asp Arg 
                805                 810                 815     


Ile Lys Phe Ser His Gln Val Asp Lys Lys Met Asn Arg Lys Val Ser 
            820                 825                 830         


Asp Ala Thr Ile Tyr Ser Thr Arg Thr Gly Lys Leu Ala Lys Asp Lys 
        835                 840                 845             


Lys Asp Ala Glu Tyr Ile Val Ala Lys Val Lys Asp Ile Tyr Ser Val 
    850                 855                 860                 


Asp Gly Phe Lys Lys Phe Lys Lys Val Tyr Asp Lys Asp Lys Ser Lys 
865                 870                 875                 880 


Phe Leu Leu Tyr Lys Tyr Asp Pro Arg Thr Phe Ser Glu Leu Glu Arg 
                885                 890                 895     


Ile Ile Ser Asp Cys Pro Asp Lys Val Glu Lys Val Gln Thr Asn Gly 
            900                 905                 910         


Lys Val Lys Ala Val Asp Val Ser Pro Phe Glu Met Tyr Arg Arg Tyr 
        915                 920                 925             


His Gly Met Ile Lys Lys Tyr Ser Lys Lys Gly Asn Gly Pro Ala Ile 
    930                 935                 940                 


Lys Gln Leu Lys Tyr Leu Asp Lys Lys Leu Gly Ser His Ile Asp Ile 
945                 950                 955                 960 


Thr Pro Ala Asn Ala Asn Gly Lys His Val Ile Leu Gln Ser Leu Lys 
                965                 970                 975     


Pro Trp Arg Thr Asp Val Tyr Leu Asn His Glu Thr Gly Glu Tyr Glu 
            980                 985                 990         


Ile Met Gly Ile Lys Tyr Ser Asp  Leu Lys Phe Asn Lys  Asn Val Gly 
        995                 1000                 1005             


Tyr Gly  Ile Lys Lys Asp Lys  Tyr Leu Glu Ile Lys  Lys Asn Glu 
    1010                 1015                 1020             


Glu Val  Ser Glu Lys Ser Glu  Phe Met Phe Ser Leu  Tyr Arg Lys 
    1025                 1030                 1035             


Asp Arg  Val Arg Ile Gln Asn  Met Lys Thr Gly Glu  Ser Val Glu 
    1040                 1045                 1050             


Leu Leu  Phe Trp Ser Arg Asn  Phe Ser Asn Lys Lys  Tyr Ala Glu 
    1055                 1060                 1065             


Leu Lys  Pro Ile Phe Lys Phe  Val Thr Glu Asp Asp  Asp Trp Pro 
    1070                 1075                 1080             


Val Tyr  Gly His Ala Lys Thr  Gln Ile Leu Lys Arg  Leu Val Pro 
    1085                 1090                 1095             


Lys Asp  Cys Lys Ile Trp Lys  Val Asn Thr Thr Ile  Leu Gly Asp 
    1100                 1105                 1110             


Pro Tyr  Tyr Leu Glu Lys Glu  Ser Asp Asn Pro Lys  Asp Ile Leu 
    1115                 1120                 1125             


Asp 
    


<210>  704
<211>  1129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  704

Met Thr Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Glu Ala Lys Thr Gly Lys Val Val His Ala Asn 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ser Arg Arg Leu Asn Arg Arg Lys Lys His Arg Val 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Gly Ile Val Thr Asp Phe 
65                  70                  75                  80  


Arg Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ser 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Thr Asp Tyr Ala Lys Ser Ile Asp Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Glu Lys Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ala Asp Tyr Asn Lys Lys Ile Thr Ala 
        195                 200                 205             


Glu Phe Ile Asp Asp Tyr Val Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Ser Phe Tyr Pro Asp Glu Tyr Arg Ala Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Tyr Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Pro Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys Glu Ala Leu Val 
    290                 295                 300                 


Glu Phe Ala Lys Ser Thr Ala Thr Leu Gly Pro Ala Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Ile Leu Asp Cys Lys Val Asp Glu Ile Lys Gly Tyr 
                325                 330                 335     


Arg Glu Asp Asp Lys Gly Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Asp Ser Val Asn Ile Asp Asp Leu Ser 
        355                 360                 365             


Arg Glu Val Leu Asp Lys Leu Ala Asp Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Ala Ile Lys Arg Asn Leu Pro Asn Gln Phe 
385                 390                 395                 400 


Thr Glu Glu Gln Ile Ser Glu Ile Ile Asn Val Arg Lys Ser Gln Ser 
                405                 410                 415     


Thr Ala Phe Asn Lys Gly Trp His Ser Phe Ser Ala Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Ala Thr Ser Asp Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Val Lys Lys Lys Ser Ser Lys Asn 
    450                 455                 460                 


Ile Lys Thr Ile Asp Glu Lys Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Thr Ile Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Asp Asp Glu Lys Lys Phe Ile Asp Lys Arg Asn Lys 
        515                 520                 525             


Glu Asn Lys Lys Glu Lys Asp Asp Ala Leu Lys Arg Ala Ala Tyr Leu 
    530                 535                 540                 


Tyr Asn Gly Thr Asp Lys Leu Pro Asp Glu Val Phe His Glu Asn Lys 
545                 550                 555                 560 


Gln Leu Glu Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Pro Ile Pro Ile Gln Glu Leu Val His Asn Ser 
            580                 585                 590         


Asn Asn Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ser Trp Thr Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Ala Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Asp Tyr Val Leu Lys Gln Lys Gly Leu 
                645                 650                 655     


Gly Lys Lys Lys Arg Asp Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Leu Gln Ser Ala Leu Arg Glu 
    690                 695                 700                 


Leu Gly Lys Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Lys 
            740                 745                 750         


Leu Trp Glu Lys Gln Asp Asn Pro Met Phe Val Asp Tyr Gly Asn Asn 
        755                 760                 765             


Gln Val Val Asp Lys Gln Thr Gly Glu Ile Leu Ser Val Ser Asp Asp 
    770                 775                 780                 


Glu Tyr Lys Glu Leu Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Lys Gly Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Tyr Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Ile Gly Lys Asp Lys Lys Glu Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Asn Gly Phe Asp Thr Phe 
    850                 855                 860                 


Val Lys Lys Tyr Asn Lys Asp Lys Thr Gln Phe Leu Met Tyr Gln Lys 
865                 870                 875                 880 


Asp Pro Leu Thr Trp Glu Asn Val Ile Glu Val Ile Leu Arg Asp Tyr 
                885                 890                 895     


Pro Thr Thr Lys Lys Ser Glu Asp Gly Lys Asn Asp Val Lys Cys Asn 
            900                 905                 910         


Pro Phe Glu Glu Tyr Arg Arg Glu Asn Gly Leu Ile Cys Lys Tyr Ser 
        915                 920                 925             


Lys Lys Gly Lys Gly Thr Pro Ile Lys Ser Leu Lys Tyr Tyr Asp Lys 
    930                 935                 940                 


Lys Leu Gly Asn Cys Ile Asp Ile Thr Pro Gln Glu Ser Lys Asn Arg 
945                 950                 955                 960 


Val Val Leu Arg Gln Ile Ser Pro Trp Arg Ala Asp Ile Tyr Phe Asn 
                965                 970                 975     


Pro Glu Thr Leu Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu 
            980                 985                 990         


Ser Phe Glu Lys Gly Thr Gly Lys  Tyr His Ile Ser Gln  Glu Lys Tyr 
        995                 1000                 1005             


Asp Ala  Ile Lys Glu Lys Glu  Gly Val Gly Lys Asp  Ser Glu Phe 
    1010                 1015                 1020             


Lys Phe  Thr Leu Tyr Arg Asn  Asp Leu Ile Leu Ile  Lys Asp Thr 
    1025                 1030                 1035             


Leu Asn  Asn Cys Glu Arg Met  Leu Arg Phe Gly Ser  Lys Asn Asp 
    1040                 1045                 1050             


Thr Ser  Lys His Tyr Val Glu  Leu Lys Pro Leu Glu  Lys Gly Thr 
    1055                 1060                 1065             


Phe Asp  Ser Glu Glu Glu Ile  Leu Pro Val Leu Gly  Lys Val Ala 
    1070                 1075                 1080             


Lys Ser  Gly Gln Phe Ile Lys  Gly Leu Asn Lys Pro  Asn Ile Ser 
    1085                 1090                 1095             


Ile Tyr  Lys Val Arg Thr Asp  Val Leu Gly Asn Lys  Tyr Phe Val 
    1100                 1105                 1110             


Lys Lys  Glu Gly Asp Lys Pro  Lys Leu Asp Phe Lys  Asn Asn Lys 
    1115                 1120                 1125             


Lys 
    


<210>  705
<211>  1129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  705

Met Ser Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Val Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Asp Ser Lys Thr Gly Asn Val Ile His Ala Asn 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ala Arg Arg Leu Thr Arg Arg Lys Lys His Arg Val 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Asp Ile Ser Thr Asp Phe 
65                  70                  75                  80  


Arg Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Ser Asp Tyr Ala Lys Ser Ile Asp Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Lys Asn Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ser Asn Tyr Asn Lys Gln Ile Thr Asp 
        195                 200                 205             


Glu Phe Ile Glu Asp Tyr Ile Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Ser Phe Tyr Pro Glu Glu Tyr Arg Ala Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Phe Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Pro Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys Glu Tyr Leu Val 
    290                 295                 300                 


Asp Phe Ala Lys Lys Ser Lys Ala Leu Gly Ala Ser Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Ile Val Asp Cys Ser Val Asp Asp Ile Lys Gly Tyr 
                325                 330                 335     


Arg Val Asp Asn Lys Asp Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Ser Ser Ile Asp Ile Asp Glu Leu Ser 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Asp Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Ala Ile Lys Arg Asn Leu Pro Ser Gln Phe 
385                 390                 395                 400 


Thr Glu Glu Gln Ile Ser Glu Ile Val Gln Ile Arg Lys Asn Gln Ser 
                405                 410                 415     


Ser Ala Phe Asn Lys Gly Trp His Ser Phe Ser Ala Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Val Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Val Asn Lys Lys Ser Ser Lys Asn 
    450                 455                 460                 


Thr Lys Thr Ile Asp Glu Lys Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Thr Ile Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Glu Asp Glu Lys Lys Phe Ile Asp Lys Lys Gln Lys 
        515                 520                 525             


Glu Asn Lys Lys Glu Lys Asp Asp Ser Leu Lys Arg Ala Ala Phe Leu 
    530                 535                 540                 


Tyr Asn Gly Thr Asp Asn Leu Pro Asp Gly Val Phe His Gly Asn Lys 
545                 550                 555                 560 


Glu Leu Lys Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Leu Ile Ser Ile His Asp Leu Val His Asn Ser 
            580                 585                 590         


Asn Lys Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Trp Thr Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Lys Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Thr Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Asp Tyr Val Leu Lys Gln Lys Gly Leu 
                645                 650                 655     


Gly Lys Lys Lys Cys Glu Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Leu Gln Thr Ala Leu Lys Glu 
    690                 695                 700                 


Leu Gly Lys Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Asn Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Lys 
            740                 745                 750         


Leu Trp Gln Lys Gln Glu Asn Pro Met Phe Glu Ser Tyr Gly Glu Asn 
        755                 760                 765             


Gln Val Val Asn Lys Glu Thr Gly Glu Ile Leu Ser Ile Ser Asp Asp 
    770                 775                 780                 


Lys Tyr Lys Glu Leu Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Lys Gly Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Phe Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Leu Gly Lys Asp Lys Lys Asp Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Asp Gly Phe Asp Thr Phe 
    850                 855                 860                 


Ile Lys Arg Tyr Lys Lys Asp Lys Thr Gln Phe Leu Met Tyr Gln Lys 
865                 870                 875                 880 


Asp Pro Leu Thr Trp Glu Asn Val Ile Glu Val Ile Leu Arg Asp Tyr 
                885                 890                 895     


Pro Ser Glu Lys Leu Ser Glu Asp Gly Lys Lys Thr Val Lys Cys Asn 
            900                 905                 910         


Pro Phe Glu Glu Tyr Arg Arg Glu Asn Gly Leu Ile Cys Lys Tyr Ser 
        915                 920                 925             


Lys Lys Gly Asn Gly Thr Pro Ile Lys Ser Leu Lys Tyr Tyr Asp Lys 
    930                 935                 940                 


Lys Leu Gly Asn Cys Ile Asp Ile Thr Pro Glu Lys Ser Lys Asn Arg 
945                 950                 955                 960 


Val Val Leu Arg Gln Ile Ser Pro Trp Arg Ala Asp Ile Tyr Phe Asn 
                965                 970                 975     


Leu Glu Thr Leu Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu 
            980                 985                 990         


Ser Phe Glu Lys Gly Thr Gly Lys  Tyr His Ile Ser Gln  Glu Lys Tyr 
        995                 1000                 1005             


Asp Ala  Ile Arg Glu Lys Glu  Gly Ile Gly Lys Lys  Ser Glu Phe 
    1010                 1015                 1020             


Lys Phe  Thr Leu Tyr Arg Asn  Asp Leu Ile Leu Ile  Lys Asp Thr 
    1025                 1030                 1035             


Leu Asn  Asn Cys Glu Arg Met  Leu Arg Phe Gly Ser  Lys Asn Asp 
    1040                 1045                 1050             


Thr Ser  Lys His Tyr Val Glu  Leu Lys Pro Leu Glu  Lys Gly Thr 
    1055                 1060                 1065             


Phe Asp  Ser Glu Glu Glu Ile  Leu Pro Val Leu Gly  Lys Val Ala 
    1070                 1075                 1080             


Lys Ser  Gly Gln Phe Ile Lys  Gly Leu Asn Lys Pro  Asn Ile Ser 
    1085                 1090                 1095             


Ile Tyr  Lys Val Arg Thr Asp  Val Leu Gly Asn Lys  Phe Phe Ile 
    1100                 1105                 1110             


Lys Lys  Glu Gly Asp Lys Pro  Lys Leu Asp Phe Lys  Asn Asn Asn 
    1115                 1120                 1125             


Lys 
    


<210>  706
<211>  1129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  706

Met Ser Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Val Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Asp Ala Lys Thr Gly Asn Val Ile His Ala Asn 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ala Arg Arg Leu Thr Arg Arg Lys Lys His Arg Val 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Asp Ile Ser Thr Asp Phe 
65                  70                  75                  80  


Arg Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Ser Asp Tyr Ala Lys Ser Ile Asp Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Lys Asn Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ser Asn Tyr Asn Lys Gln Ile Thr Asp 
        195                 200                 205             


Glu Phe Ile Glu Asp Tyr Ile Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Ser Phe Tyr Pro Glu Glu Tyr Arg Ala Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Phe Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Pro Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys Glu Tyr Leu Val 
    290                 295                 300                 


Asp Phe Ala Lys Lys Ser Lys Ala Leu Gly Ala Ser Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Ile Val Asp Cys Ser Val Asp Asp Ile Lys Gly Tyr 
                325                 330                 335     


Arg Val Asp Asn Lys Asp Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Ser Ser Ile Asp Ile Asp Glu Leu Ser 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Asp Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Thr Ile Lys Arg Asn Leu Pro Ser Gln Phe 
385                 390                 395                 400 


Thr Glu Glu Gln Ile Ser Glu Ile Val Gln Ile Arg Lys Asn Gln Ser 
                405                 410                 415     


Ser Ala Phe Asn Lys Gly Trp His Ser Phe Ser Ala Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Val Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Val Asn Lys Lys Ser Ser Lys Asn 
    450                 455                 460                 


Thr Lys Thr Ile Asp Glu Lys Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Thr Ile Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Glu Asp Glu Lys Lys Phe Ile Asp Lys Lys Glu Lys 
        515                 520                 525             


Glu Asn Lys Lys Glu Lys Asp Asp Ser Leu Lys Arg Ala Ala Phe Leu 
    530                 535                 540                 


Tyr Asn Gly Thr Asp Asn Leu Pro Asp Gly Val Phe His Gly Asn Lys 
545                 550                 555                 560 


Glu Leu Lys Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Leu Ile Ser Ile His Asp Leu Val His Asn Ser 
            580                 585                 590         


Asn Lys Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Trp Thr Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Lys Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Ala Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Asp Tyr Val Leu Lys Gln Lys Gly Ile 
                645                 650                 655     


Gly Lys Lys Lys Arg Glu Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Leu Gln Thr Ala Leu Lys Glu 
    690                 695                 700                 


Leu Gly Lys Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Gly Gln Leu Lys 
            740                 745                 750         


Leu Trp Gln Lys His Glu Asn Leu Met Phe Glu Asn Tyr Gly Glu Asn 
        755                 760                 765             


Gln Val Val Asn Lys Glu Thr Gly Glu Ile Leu Ser Ile Ser Asp Asp 
    770                 775                 780                 


Glu Tyr Lys Glu Leu Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Lys Thr Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Phe Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Leu Gly Lys Asp Lys Lys Glu Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Thr Gln Asp Gly Phe Asp Thr Phe 
    850                 855                 860                 


Ile Lys Arg Tyr Lys Lys Asp Lys Thr Gln Phe Leu Met Tyr Gln Lys 
865                 870                 875                 880 


Asp Pro Leu Thr Trp Glu Asn Val Ile Glu Val Ile Leu Arg Asp Tyr 
                885                 890                 895     


Pro Ser Glu Lys Leu Ser Glu Asp Gly Lys Lys Thr Val Lys Cys Asn 
            900                 905                 910         


Pro Phe Glu Glu Tyr Arg Arg Glu Asn Gly Leu Ile Cys Lys Tyr Ser 
        915                 920                 925             


Lys Lys Gly Asn Gly Thr Pro Ile Lys Ser Leu Lys Tyr Tyr Asp Lys 
    930                 935                 940                 


Lys Leu Gly Asn Cys Ile Asp Ile Thr Pro Glu Lys Ser Lys Asn Arg 
945                 950                 955                 960 


Val Val Leu Arg Gln Ile Ser Pro Trp Arg Ala Asp Ile Tyr Phe Asn 
                965                 970                 975     


Leu Glu Thr Leu Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu 
            980                 985                 990         


Ser Phe Glu Lys Gly Thr Gly Lys  Tyr His Ile Ser Gln  Glu Lys Tyr 
        995                 1000                 1005             


Asp Ala  Ile Arg Glu Lys Glu  Gly Ile Gly Lys Lys  Ser Glu Phe 
    1010                 1015                 1020             


Lys Phe  Thr Leu Tyr Arg Asn  Asp Leu Ile Leu Ile  Lys Asp Thr 
    1025                 1030                 1035             


Leu Asn  Asn Cys Glu Arg Met  Leu Arg Phe Gly Ser  Lys Asn Asp 
    1040                 1045                 1050             


Thr Ser  Lys His Tyr Val Glu  Leu Lys Pro Leu Glu  Lys Gly Thr 
    1055                 1060                 1065             


Phe Asp  Ser Glu Glu Glu Ile  Leu Pro Val Leu Gly  Lys Val Ala 
    1070                 1075                 1080             


Lys Ser  Gly Gln Phe Ile Lys  Gly Leu Asn Lys Pro  Asn Ile Ser 
    1085                 1090                 1095             


Ile Tyr  Lys Val Arg Thr Asp  Val Leu Gly Asn Lys  Phe Phe Ile 
    1100                 1105                 1110             


Lys Lys  Glu Gly Asp Lys Pro  Lys Leu Asp Phe Lys  Asn Asn Asn 
    1115                 1120                 1125             


Lys 
    


<210>  707
<211>  1130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  707

Met Asn Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Asn Asn Ile Ile Leu Gly Met Gly Ser Arg Val Phe 
            20                  25                  30          


Pro Glu Gly Val Val Asn Leu Gly Glu Gly Glu Gly Arg Glu Thr Ser 
        35                  40                  45              


Lys Asn Ala Ser Arg Thr Glu Ala Arg Gly Val Arg Arg Gln Ile Phe 
    50                  55                  60                  


Arg Arg Arg Leu Arg Lys Arg Tyr Leu Leu Lys Glu Leu Ala Lys His 
65                  70                  75                  80  


Asn Leu Cys Pro Ile Ser Pro Thr Leu Ile Lys Asp Trp Asn Gly Thr 
                85                  90                  95      


Asp Ile Phe Lys Asn Glu Leu Ile Ile Gln Trp Phe Gln Leu Asn Pro 
            100                 105                 110         


Tyr Glu Leu Arg Ala Lys Ala Ile Gln Glu Glu Ile Thr Leu Ile Glu 
        115                 120                 125             


Leu Gly Arg Ile Phe Tyr His Met Ile Gln Arg Arg Gly Phe Leu Ser 
    130                 135                 140                 


Asn Ser Arg Ser Ala Gly Ala Asp Val Lys Glu Thr Ser Thr Ile Phe 
145                 150                 155                 160 


Lys Gly Asp Ala Lys Ile Gly Lys Ile Gly Ile Ser Asp Thr Leu Lys 
                165                 170                 175     


Ser Ile Glu Gln Asn Lys Thr Leu Gly Ser Phe Leu His Ser Ile Gln 
            180                 185                 190         


Pro Lys Glu Asn Glu Pro Phe Ser Gly Glu Leu Glu Arg Ile Arg Asn 
        195                 200                 205             


Arg Tyr Thr Thr Arg Gln Met Tyr Ile Asp Glu Phe Glu Thr Ile Trp 
    210                 215                 220                 


Glu Phe Gln Lys Gln Tyr His Ser Glu Leu Asn Ser Asn Leu Lys Thr 
225                 230                 235                 240 


Ile Leu Gly Gly Arg Lys Lys Asp Asn Tyr Lys Glu Asp Gly Val Leu 
                245                 250                 255     


Phe His Gln Arg Pro Leu Arg Ser Gln Lys His Leu Val Gly Phe Cys 
            260                 265                 270         


Ser Phe Glu Pro Lys Lys Thr Lys Cys Lys Leu Ser Ala Ile Pro Phe 
        275                 280                 285             


Glu Leu Phe Arg Ile Tyr Gln Trp Val Asn Thr Leu Ser Tyr Asp Val 
    290                 295                 300                 


Asn Gly Val Arg Gln Lys Ile Thr Gln Glu Glu Arg Lys Lys Ile Ile 
305                 310                 315                 320 


Asp Leu Leu Leu Ser Lys Glu Lys Pro Ser Phe Lys Glu Ile Arg Lys 
                325                 330                 335     


Ala Ile Gly Lys Leu Asp Gly Ser Tyr Gln Phe Asn Tyr Lys Asp Asp 
            340                 345                 350         


Asp Lys Ile Val Gly Ser His Thr Ile Ser Asn Leu Ser Asn Lys Lys 
        355                 360                 365             


Phe Phe Gly Asn Lys Trp Phe Asp Phe Ser Glu Lys Glu Gln Glu Asn 
    370                 375                 380                 


Ile Trp His Ile Leu Ser Phe Phe Asp Asp Arg Glu Lys Leu Lys Glu 
385                 390                 395                 400 


Tyr Ala Ile Lys Asn Trp Ser Phe Asp Glu Glu Arg Ala Val Lys Ile 
                405                 410                 415     


Ser Lys Phe Asn Leu Lys Asp Gly Tyr Ser Asn Leu Ser Arg Lys Ala 
            420                 425                 430         


Ile Leu Asn Ile Leu Pro Phe Leu Gln Lys Gly Phe Met Tyr Asp Val 
        435                 440                 445             


Ala Val Gly Leu Ala Gly Val Lys Asn Thr Leu Ser Val Asn Trp Glu 
    450                 455                 460                 


Glu His Glu Gln Phe Val Leu Asp Asn Val Pro Glu Ile Val Arg Ser 
465                 470                 475                 480 


Asn Leu Lys Gly Gly Tyr Ile Glu Pro Leu Lys Asn Met Leu Lys Asn 
                485                 490                 495     


Glu Phe Asn Cys Asn Asp Lys Gln Leu Glu Lys Leu Tyr His His Ser 
            500                 505                 510         


Ser Ala Ile Glu Thr Glu Asn Ile Leu Asp Arg Leu Pro Val Asn Leu 
        515                 520                 525             


His Ala Asp Arg Glu Ile Gln Lys Ile Lys Asn Pro Val Val Ile Thr 
    530                 535                 540                 


Ala Leu Phe Glu Ile Arg Lys Leu Val Asn Glu Val Ile Asp Thr Tyr 
545                 550                 555                 560 


Gly Lys Pro Asn Glu Ile Lys Val Glu Leu Ala Arg Asp Leu Lys Ala 
                565                 570                 575     


Ser Lys Ser Asn Arg Leu Glu Ala Arg Lys Arg Gln Lys Asp Leu Glu 
            580                 585                 590         


Lys Gln Asn Asp Arg Val Lys Leu Glu Leu Glu Asn Leu Asn Gln Arg 
        595                 600                 605             


Ser Asn His Ile Asn Ile Leu Lys Tyr Lys Leu Trp Glu Glu Cys Asn 
    610                 615                 620                 


His Thr Cys Pro Phe Thr Gly Gln Ile Ile Thr Ile Ser Gln Leu Phe 
625                 630                 635                 640 


Thr Gly Glu Val Gln Ile Glu His Ile Phe Pro Trp Ser Arg Ser Leu 
                645                 650                 655     


Asn Asp Ser Phe Asn Asn Lys Thr Leu Cys Phe Ala Asp Glu Asn Arg 
            660                 665                 670         


Ala Lys Gly Asn Lys Thr Pro Tyr Glu Leu Tyr Ser Glu Gln Gly Asp 
        675                 680                 685             


Gln Lys Trp Glu Ala Ile Lys Leu Gln Ala Leu Ser Cys Phe Lys Thr 
    690                 695                 700                 


Lys Ala Asn Tyr Pro Asn Cys Tyr Asn Lys Phe Lys His Phe Val Lys 
705                 710                 715                 720 


Lys Lys His Asp Glu Asp Phe Ile Ser Arg Gln Leu Asn Asp Thr Arg 
                725                 730                 735     


Tyr Ile Ser Lys Glu Ala Lys Asn Tyr Leu Ser Lys Ile Cys Ser Asn 
            740                 745                 750         


Val Leu Val Ala Pro Gly Gln Met Thr Ala Thr Leu Arg His His Trp 
        755                 760                 765             


Gly Leu Asn Ser Ile Leu Asn Leu Glu Asp Asp Thr Lys Thr Arg Asp 
    770                 775                 780                 


Asp His Arg His His Ala Ile Asp Ala Leu Val Met Ala Cys Ser Thr 
785                 790                 795                 800 


Arg Ser His Leu Gln Glu Leu Ser Lys Arg Asn Arg Tyr Asn Lys Asn 
                805                 810                 815     


Tyr Asp Leu Lys Asp Phe Pro Asp Pro Trp Gln Asn Phe Arg Gln Asp 
            820                 825                 830         


Ala Glu Asn Ala Val Ala Gln Ile Leu Val Ser His Lys Lys Ser Lys 
        835                 840                 845             


Ser Leu Leu Thr Val Arg Thr His Lys Thr Ile Lys Lys Gly Val Glu 
    850                 855                 860                 


His Lys Asn Ile Gly Val Ala Ala Arg Gly Gln Leu His Lys Glu Ser 
865                 870                 875                 880 


Val Tyr Gly Leu Arg Lys Ala Pro Asn Ser Asn Lys Ala Phe His Ile 
                885                 890                 895     


Arg Lys Pro Leu Glu Ser Leu Thr Thr Glu Lys Gln Leu Glu Lys Ile 
            900                 905                 910         


Val Asp Asp Ser Ile Arg Leu Leu Ile Leu Lys Arg Ile Asn Ile Ile 
        915                 920                 925             


Gly Gly Phe Ile Lys Gly Lys Ile Pro Lys Glu Thr Phe Phe Val Val 
    930                 935                 940                 


Asp Glu Asn Gly Ile Lys Gln Pro Gln Ile Phe Leu Pro Asn Arg Asn 
945                 950                 955                 960 


Gly Asn Pro Val Pro Ile Lys Lys Val Arg Ile Arg Glu Asn Ile Gly 
                965                 970                 975     


Gly Ala Glu Lys Leu Lys Glu Asn Thr Asn Gln Tyr Val Asn Pro Arg 
            980                 985                 990         


Ser Asn His His Val Ile Val Tyr  Glu Asp Ser Asn Gly  Gly Leu Lys 
        995                 1000                 1005             


Asp Asp  Val Val Thr Phe Trp  Thr Ala Val Glu Arg  Lys Arg Gln 
    1010                 1015                 1020             


Gly Gln  Asp Val Phe Lys Leu  Pro Asn Asp Gly Glu  Lys Ile Val 
    1025                 1030                 1035             


Ser Thr  Leu Gln Ile Asn Asp  Met Phe Leu Leu Gly  Leu Asn Asp 
    1040                 1045                 1050             


Asp Asp  Ile Ile Thr Leu Thr  Ser Lys Glu Leu Lys  Lys His Leu 
    1055                 1060                 1065             


Phe Lys  Val Gln Thr Leu Ser  Ser Ser Phe Tyr Glu  Phe Arg Leu 
    1070                 1075                 1080             


Ser Thr  Asp Ser Thr Gln Asn  Lys Asn Thr Asn Ser  Thr Val Phe 
    1085                 1090                 1095             


Arg Arg  Ile Gln Ser Phe Gly  Met Gly Lys Thr Gly  Trp Lys Ala 
    1100                 1105                 1110             


Phe Asn  Pro Ile Lys Val Lys  Leu Asn Ser Ile Gly  Glu Val Lys 
    1115                 1120                 1125             


Ile Ile  
    1130 


<210>  708
<211>  1130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  708

Met Thr Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Glu Ala Lys Thr Gly Lys Val Val His Ala Asn 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ser Arg Arg Leu Asn Arg Arg Lys Lys His Arg Val 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Gly Ile Val Thr Asp Phe 
65                  70                  75                  80  


Arg Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ser 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Thr Asp Tyr Ala Lys Ser Ile Asp Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Glu Lys Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ala Asp Tyr Asn Lys Lys Ile Thr Ala 
        195                 200                 205             


Glu Phe Ile Asp Asp Tyr Val Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Ser Phe Tyr Pro Asp Glu Tyr Arg Ala Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Tyr Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Pro Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys Glu Ala Leu Val 
    290                 295                 300                 


Glu Phe Ala Lys Ser Thr Ala Thr Leu Gly Pro Ala Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Ile Leu Asp Cys Lys Val Asp Glu Ile Lys Gly Tyr 
                325                 330                 335     


Arg Glu Asp Asp Lys Gly Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Asp Ser Val Asn Ile Asp Asp Leu Ser 
        355                 360                 365             


Arg Glu Val Leu Asp Lys Leu Ala Asp Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Ala Ile Lys Arg Asn Leu Pro Asn Gln Phe 
385                 390                 395                 400 


Thr Glu Glu Gln Ile Ser Glu Ile Ile Asn Val Arg Lys Ser Gln Ser 
                405                 410                 415     


Thr Ala Phe Asn Lys Gly Trp His Ser Phe Ser Ala Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Ala Thr Ser Asp Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Val Lys Lys Lys Ser Ser Lys Asn 
    450                 455                 460                 


Ile Lys Thr Ile Asp Glu Lys Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Thr Ile Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Asp Asp Glu Lys Lys Phe Ile Asp Lys Arg Asn Lys 
        515                 520                 525             


Glu Asn Lys Lys Glu Lys Asp Asp Ala Leu Lys Arg Ala Ala Tyr Leu 
    530                 535                 540                 


Tyr Asn Gly Thr Asp Lys Leu Pro Asp Glu Val Phe His Gly Asn Lys 
545                 550                 555                 560 


Gln Leu Glu Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Pro Ile Pro Ile Gln Glu Leu Val His Asn Ser 
            580                 585                 590         


Asn Asn Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ser Trp Thr Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Arg Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Ala Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Asp Tyr Val Leu Lys Gln Lys Gly Leu 
                645                 650                 655     


Gly Lys Lys Lys Arg Asp Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Leu Gln Ser Ala Leu Arg Glu 
    690                 695                 700                 


Leu Gly Lys Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Lys 
            740                 745                 750         


Leu Trp Glu Lys Gln Asp Asn Pro Met Phe Val Asp Tyr Gly Asn Asn 
        755                 760                 765             


Gln Val Val Asp Lys Gln Thr Gly Glu Ile Leu Ser Val Ser Asp Asn 
    770                 775                 780                 


Glu Tyr Lys Glu Leu Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Lys Gly Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Tyr Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Ile Gly Lys Asp Lys Lys Glu Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Asn Gly Phe Asp Thr Phe 
    850                 855                 860                 


Val Lys Lys Tyr Asn Lys Asp Lys Thr Gln Phe Leu Met Tyr Gln Lys 
865                 870                 875                 880 


Asp Pro Leu Thr Trp Glu Asn Val Ile Glu Val Ile Leu Gly Asp Tyr 
                885                 890                 895     


Pro Thr Thr Lys Lys Ser Glu Asp Gly Lys Asn Asp Val Lys Cys Asn 
            900                 905                 910         


Pro Phe Glu Glu Tyr Arg Arg Glu Asn Gly Leu Ile Cys Lys Tyr Ser 
        915                 920                 925             


Lys Lys Gly Lys Gly Thr Pro Ile Lys Ser Leu Lys Tyr Tyr Asp Lys 
    930                 935                 940                 


Lys Leu Gly Asn Cys Ile Asp Ile Thr Pro Glu Glu Ser Arg Asn Lys 
945                 950                 955                 960 


Val Ile Leu Gln Ser Ile Asn Pro Trp Arg Ala Asp Val Tyr Phe Asn 
                965                 970                 975     


Pro Glu Thr Leu Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu 
            980                 985                 990         


Ser Phe Glu Lys Gly Thr Gly Asn  Tyr His Ile Ser Gln  Glu Lys Tyr 
        995                 1000                 1005             


Asp Ala  Ile Lys Glu Lys Glu  Gly Ile Gly Lys Lys  Ser Glu Phe 
    1010                 1015                 1020             


Lys Phe  Thr Leu Tyr Arg Asn  Asp Leu Ile Leu Ile  Lys Asp Ile 
    1025                 1030                 1035             


Ala Ser  Gly Glu Gln Glu Ile  Tyr Arg Phe Leu Ser  Arg Thr Met 
    1040                 1045                 1050             


Pro Asn  Val Asn His Tyr Val  Glu Leu Lys Pro Tyr  Asp Lys Glu 
    1055                 1060                 1065             


Lys Phe  Asp Asn Val Gln Glu  Leu Val Glu Ala Leu  Gly Glu Ala 
    1070                 1075                 1080             


Asp Lys  Val Gly Arg Cys Ile  Lys Gly Leu Asn Lys  Pro Asn Ile 
    1085                 1090                 1095             


Ser Ile  Tyr Lys Val Arg Thr  Asp Val Leu Gly Asn  Lys Tyr Phe 
    1100                 1105                 1110             


Val Lys  Lys Glu Gly Asp Lys  Pro Lys Leu Asp Phe  Lys Asn Asn 
    1115                 1120                 1125             


Lys Lys  
    1130 


<210>  709
<211>  1130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  709

Met Thr Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Glu Ala Lys Thr Gly Lys Val Val His Ala Asn 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ser Arg Arg Leu Asn Arg Arg Lys Lys His Arg Val 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Glu Ile Val Thr Asp Phe 
65                  70                  75                  80  


Arg Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ser 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Thr Asp Tyr Ala Lys Ser Ile Asp Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Glu Lys Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ala Asp Tyr Asn Lys Lys Ile Thr Ala 
        195                 200                 205             


Glu Phe Ile Asp Asp Tyr Val Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Ser Phe Tyr Pro Asp Glu Tyr Arg Ala Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Tyr Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Pro Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys Glu Ala Leu Val 
    290                 295                 300                 


Glu Phe Ala Lys Ser Thr Ala Thr Leu Gly Pro Ala Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Ile Leu Asp Cys Lys Val Asp Glu Ile Lys Gly Tyr 
                325                 330                 335     


Arg Glu Asp Asp Lys Gly Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Asp Ser Val Asn Ile Asp Asp Leu Ser 
        355                 360                 365             


Arg Glu Val Leu Asp Lys Leu Ala Asp Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Ala Ile Arg His Asn Leu Pro Asn Gln Phe 
385                 390                 395                 400 


Thr Glu Gly Gln Ile Ser Glu Ile Ile Lys Val Arg Lys Ser Gln Ser 
                405                 410                 415     


Thr Ala Phe Asn Lys Gly Trp His Ser Phe Ser Ala Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Ala Thr Ser Asp Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Val Asn Lys Lys Ser Ser Lys Asn 
    450                 455                 460                 


Thr Lys Thr Ile Asp Glu Lys Glu Val Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Thr Ile Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Asp Asp Glu Lys Lys Phe Ile Asp Lys Arg Asn Lys 
        515                 520                 525             


Glu Asn Lys Lys Glu Lys Asp Asp Ala Leu Lys Arg Ala Ala Tyr Leu 
    530                 535                 540                 


Tyr Asn Gly Thr Asp Lys Leu Pro Asp Glu Val Phe His Gly Asn Lys 
545                 550                 555                 560 


Gln Leu Glu Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Pro Ile Pro Ile Gln Glu Leu Val His Asn Ser 
            580                 585                 590         


Asn Asn Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Trp Thr Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Lys Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Ala Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Asp Tyr Val Leu Lys Gln Lys Gly Leu 
                645                 650                 655     


Gly Lys Lys Lys Arg Asp Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Leu Gln Ser Ala Leu Arg Glu 
    690                 695                 700                 


Leu Gly Lys Asp Thr Lys Ile Ser Val Ile Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Lys 
            740                 745                 750         


Leu Trp Glu Lys Gln Asp Asn Pro Met Phe Val Asp Tyr Gly Asn Asn 
        755                 760                 765             


Gln Val Val Asp Lys Gln Thr Gly Glu Ile Leu Ser Val Ser Asp Asp 
    770                 775                 780                 


Glu Tyr Lys Glu Leu Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Met Ile Ser Ser Lys Gly Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Tyr Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Ile Gly Lys Asp Lys Lys Glu Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Asn Gly Phe Asp Thr Phe 
    850                 855                 860                 


Ile Lys Lys Tyr Asn Lys Asp Lys Thr Gln Phe Leu Met Tyr Gln Lys 
865                 870                 875                 880 


Asp Pro Leu Thr Trp Glu Asn Val Ile Glu Val Ile Leu Arg Asp Tyr 
                885                 890                 895     


Pro Thr Thr Lys Lys Ser Glu Asp Gly Lys Asn Asp Val Lys Cys Asn 
            900                 905                 910         


Pro Phe Glu Glu Tyr Arg Arg Glu Asn Gly Leu Val Cys Lys Tyr Ser 
        915                 920                 925             


Lys Lys Gly Lys Gly Thr Pro Ile Lys Ser Leu Lys Tyr Tyr Asp Lys 
    930                 935                 940                 


Lys Leu Gly Asn Cys Ile Asp Ile Thr Pro Glu Gly Ser Lys Asn Glu 
945                 950                 955                 960 


Val Val Leu Gln Ser Leu Asn Pro Trp Arg Ala Asp Val Tyr Phe Asn 
                965                 970                 975     


Pro Glu Thr Leu Lys Tyr Glu Leu Leu Gly Leu Lys Tyr Ser Asp Leu 
            980                 985                 990         


Ser Phe Glu Lys Gly Thr Gly Lys  Tyr His Ile Ser Gln  Glu Lys Tyr 
        995                 1000                 1005             


Asp Val  Ile Lys Glu Lys Glu  Gly Ile Gly Lys Lys  Ser Glu Phe 
    1010                 1015                 1020             


Lys Phe  Thr Leu Tyr Arg Asn  Asp Leu Ile Leu Ile  Lys Asp Thr 
    1025                 1030                 1035             


Ala Ser  Gly Glu Gln Glu Ile  Tyr Arg Phe Leu Ser  Arg Thr Met 
    1040                 1045                 1050             


Pro Asn  Val Lys His Tyr Ala  Glu Leu Lys Pro Tyr  Asp Lys Glu 
    1055                 1060                 1065             


Lys Phe  Asp Asn Val Gln Glu  Leu Val Glu Ala Leu  Gly Glu Ala 
    1070                 1075                 1080             


Asp Lys  Val Gly Arg Cys Ile  Lys Gly Leu Asn Lys  Ser Asn Leu 
    1085                 1090                 1095             


Ser Ile  Tyr Lys Val Arg Thr  Asp Val Leu Gly Asn  Lys Tyr Phe 
    1100                 1105                 1110             


Val Lys  Lys Glu Gly Asp Lys  Pro Lys Leu Asp Phe  Lys Asn Asn 
    1115                 1120                 1125             


Lys Lys  
    1130 


<210>  710
<211>  1130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  710

Met Thr Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Glu Ala Lys Thr Gly Lys Val Val His Ala Asn 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ser Arg Arg Leu Asn Arg Arg Lys Lys His Arg Val 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Gly Ile Val Thr Asp Phe 
65                  70                  75                  80  


Arg Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ser 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Thr Asp Tyr Ala Lys Ser Ile Asp Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Glu Lys Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ala Asp Tyr Asn Lys Lys Ile Thr Ala 
        195                 200                 205             


Glu Phe Ile Asp Asp Tyr Val Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Asn Phe Tyr Pro Asp Glu Tyr Arg Ala Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Tyr Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Pro Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys Glu Ser Leu Val 
    290                 295                 300                 


Glu Phe Ala Lys Asn Thr Ala Ile Leu Gly Pro Ala Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Ile Leu Asp Cys Lys Val Asp Glu Ile Lys Gly Tyr 
                325                 330                 335     


Arg Glu Asp Asp Lys Gly Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Asp Ser Val Asn Ile Asp Tyr Leu Ser 
        355                 360                 365             


Arg Glu Val Leu Asp Lys Leu Ala Asp Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Ala Ile Lys Arg Asn Leu Pro Asn Gln Phe 
385                 390                 395                 400 


Thr Glu Glu Gln Ile Ser Glu Ile Ile Asn Val Arg Lys Ser Gln Ser 
                405                 410                 415     


Thr Ala Phe Asn Lys Gly Trp His Ser Phe Ser Ala Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Ala Thr Ser Asp Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Val Lys Lys Lys Ser Ser Lys Asn 
    450                 455                 460                 


Ile Lys Thr Ile Asp Glu Lys Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Thr Ile Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Asp Asp Glu Lys Lys Phe Ile Asn Lys Arg Asn Lys 
        515                 520                 525             


Glu Asn Lys Lys Glu Lys Asp Asp Ala Leu Lys Arg Ala Ala Tyr Leu 
    530                 535                 540                 


Tyr Asn Gly Thr Asp Lys Leu Pro Asp Glu Val Phe His Gly Asn Lys 
545                 550                 555                 560 


Gln Leu Glu Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Pro Ile Pro Ile Gln Glu Leu Val His Asn Ser 
            580                 585                 590         


Asn Asn Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ser Trp Thr Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Lys Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Ala Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Glu Tyr Val Leu Lys Gln Lys Gly Leu 
                645                 650                 655     


Gly Lys Lys Lys Arg Asp Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Leu Gln Ser Ala Leu Arg Glu 
    690                 695                 700                 


Leu Gly Lys Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Lys 
            740                 745                 750         


Leu Trp Glu Lys Gln Asp Asn Pro Met Phe Val Asp Tyr Gly Asn Asn 
        755                 760                 765             


Gln Val Val Asp Lys Gln Thr Gly Glu Ile Leu Ser Val Ser Asp Asn 
    770                 775                 780                 


Glu Tyr Lys Glu Leu Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Lys Gly Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Tyr Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Ile Gly Lys Asp Lys Lys Glu Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Asn Gly Phe Asp Thr Phe 
    850                 855                 860                 


Val Lys Lys Tyr Asn Lys Asp Lys Thr Gln Phe Leu Met Tyr Gln Lys 
865                 870                 875                 880 


Asp Pro Leu Thr Trp Glu Asn Val Ile Glu Val Ile Leu Gly Asp Tyr 
                885                 890                 895     


Pro Thr Thr Lys Lys Ser Glu Asp Gly Lys Asn Asp Val Lys Cys Asn 
            900                 905                 910         


Pro Phe Glu Glu Tyr Arg Arg Glu Asn Gly Leu Ile Cys Lys Tyr Ser 
        915                 920                 925             


Lys Lys Gly Lys Gly Thr Pro Ile Lys Ser Leu Lys Tyr Tyr Asp Lys 
    930                 935                 940                 


Lys Leu Gly Asn Cys Ile Asp Ile Thr Pro Glu Glu Ser Arg Asn Lys 
945                 950                 955                 960 


Val Ile Leu Gln Ser Ile Asn Pro Trp Arg Ala Asp Val Tyr Phe Asn 
                965                 970                 975     


Pro Glu Thr Leu Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu 
            980                 985                 990         


Ser Phe Glu Lys Gly Thr Gly Asn  Tyr His Ile Ser Gln  Glu Lys Tyr 
        995                 1000                 1005             


Asp Ala  Ile Lys Glu Lys Glu  Gly Ile Gly Lys Lys  Ser Glu Phe 
    1010                 1015                 1020             


Lys Phe  Thr Leu Tyr Arg Asn  Asp Leu Ile Leu Ile  Lys Asp Ile 
    1025                 1030                 1035             


Ala Ser  Gly Glu Gln Glu Ile  Tyr Arg Phe Leu Ser  Arg Thr Met 
    1040                 1045                 1050             


Pro Asn  Val Asn His Tyr Val  Glu Leu Lys Pro Tyr  Asp Lys Glu 
    1055                 1060                 1065             


Lys Phe  Asp Asn Val Gln Glu  Leu Val Glu Ala Leu  Gly Glu Ala 
    1070                 1075                 1080             


Asp Lys  Val Gly Arg Cys Ile  Lys Ser Leu Asn Lys  Pro Asn Ile 
    1085                 1090                 1095             


Ser Ile  Tyr Lys Val Arg Thr  Asp Val Leu Gly Asn  Lys Tyr Phe 
    1100                 1105                 1110             


Val Lys  Lys Glu Gly Asp Lys  Pro Lys Leu Asp Phe  Lys Asn Asn 
    1115                 1120                 1125             


Lys Lys  
    1130 


<210>  711
<211>  1130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  711

Met Ser Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Val Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Asp Ala Lys Thr Gly Lys Val Ile His Ala Ser 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ala Arg Arg Leu Thr Arg Arg Lys Lys His Arg Ile 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Glu Ile Val Thr Asp Phe 
65                  70                  75                  80  


Ser Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Ser Asp Tyr Ala Lys Ser Ile Glu Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Asn Met Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Lys Asn Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ser Asn Tyr Asn Lys Gln Ile Thr Asp 
        195                 200                 205             


Glu Phe Ile Glu Asp Tyr Ile Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Tyr 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Thr Phe Tyr Pro Asp Glu Tyr Arg Thr Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Tyr Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Pro Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys His Tyr Leu Val 
    290                 295                 300                 


Asp Phe Ala Lys Ser Ser Lys Ala Leu Gly Val Ala Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Ile Val Glu Cys Ser Val Asp Asp Ile Lys Gly Tyr 
                325                 330                 335     


Arg Glu Asp Asn Lys Gly Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Ser Leu Ile Asp Ile Asp Glu Leu Ser 
        355                 360                 365             


Arg Glu Thr Leu Asp Lys Leu Ala Asp Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Ala Ile Lys Arg Asn Leu Pro Asn Gln Phe 
385                 390                 395                 400 


Thr Glu Glu Gln Ile Val Glu Leu Val Asn Val Arg Lys Asn Gln Ser 
                405                 410                 415     


Ser Ala Phe Asn Lys Gly Trp His Ser Phe Ser Val Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Ala Ser Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Val Asn Lys Lys Ser Ser Glu Asn 
    450                 455                 460                 


Thr Lys Thr Ile Asn Glu Lys Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Ser Gln Thr Val Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Asp Asp Glu Lys Lys Phe Ile Asp Lys Lys Gln Lys 
        515                 520                 525             


Glu Asn Ala Lys Glu Val Glu Asp Ala Leu Lys Arg Ala Ala Tyr Leu 
    530                 535                 540                 


Tyr Asn Gly Thr Asp Lys Leu Pro Asp Gly Val Phe His Gly Asn Lys 
545                 550                 555                 560 


Gln Leu Lys Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Pro Ile Ser Ile Gln Asp Leu Val His Asn Ser 
            580                 585                 590         


Asn Asn Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Trp Thr Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Lys Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Ala Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Asp Tyr Val Leu Asn Gln Lys Gly Ile 
                645                 650                 655     


Gly Lys Lys Lys Arg Glu Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Ile Gln Ala Ala Leu Lys Glu 
    690                 695                 700                 


Leu Asp Lys Asp Thr Lys Val Ser Val Ile Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Lys 
            740                 745                 750         


Leu Trp Gln Lys Gln Glu Asn Pro Met Phe Val Asp Tyr Gly Glu Asn 
        755                 760                 765             


Gln Val Val Asn Lys Glu Thr Gly Glu Ile Leu Ser Val Ser Asp Asp 
    770                 775                 780                 


Asp Tyr Lys Glu Leu Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Lys Ala Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Tyr Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Leu Gly Lys Asp Lys Lys Glu Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Asp Gly Phe Asp Ala Phe 
    850                 855                 860                 


Ile Lys Arg Tyr Lys Lys Asp Lys Thr Gln Phe Leu Met Tyr Gln Lys 
865                 870                 875                 880 


Asp Pro Val Thr Trp Glu Asn Val Ile Glu Val Ile Leu Arg Asp Tyr 
                885                 890                 895     


Pro Ser Glu Lys Leu Ser Glu Asp Gly Lys Lys Asn Val Lys Cys Asn 
            900                 905                 910         


Pro Phe Glu Glu Tyr Arg Arg Glu Asn Gly Leu Ile Cys Lys Tyr Ser 
        915                 920                 925             


Lys Lys Gly Lys Gly Thr Pro Ile Lys Ser Leu Lys Tyr Tyr Asp Lys 
    930                 935                 940                 


Lys Leu Gly Asn Cys Ile Asp Ile Thr Pro Lys Gly Ser Lys Asn Glu 
945                 950                 955                 960 


Val Val Leu Gln Ser Leu Asn Pro Trp Arg Ala Asp Leu Tyr Phe Asn 
                965                 970                 975     


Pro Asp Thr Leu Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu 
            980                 985                 990         


Ser Phe Glu Lys Gly Thr Gly Lys  Tyr His Ile Ser Gln  Glu Arg Tyr 
        995                 1000                 1005             


Asp Glu  Ile Lys Val Lys Glu  Gly Ile Gly Lys Asn  Ser Glu Phe 
    1010                 1015                 1020             


Lys Phe  Thr Leu Tyr Arg Asn  Asp Leu Ile Leu Ile  Lys Asp Thr 
    1025                 1030                 1035             


Glu Ser  Gly Glu Glu Glu Leu  Tyr Arg Phe Leu Ser  Arg Thr Met 
    1040                 1045                 1050             


Pro Asn  Val Lys His Tyr Val  Glu Leu Lys Pro Tyr  Asp Lys Glu 
    1055                 1060                 1065             


Arg Tyr  Asp Gly Gly Gln Glu  Leu Met Lys Val Phe  Gly Asn Val 
    1070                 1075                 1080             


Ala Asn  Gly Gly Gln Cys Leu  Lys Ser Leu Asn Lys  Ser Asn Leu 
    1085                 1090                 1095             


Ser Ile  Tyr Lys Val Arg Thr  Asp Val Leu Gly Asn  Lys Phe Phe 
    1100                 1105                 1110             


Ile Lys  Lys Glu Gly Asp Lys  Pro Lys Leu Asp Phe  Lys Asn Asn 
    1115                 1120                 1125             


Lys Lys  
    1130 


<210>  712
<211>  1130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  712

Met Thr Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Glu Ala Lys Thr Gly Lys Val Val His Ala Asn 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ser Arg Arg Leu Asn Arg Arg Lys Lys His Arg Val 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Gly Ile Val Thr Asp Phe 
65                  70                  75                  80  


Arg Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Lys Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ser 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Thr Asp Tyr Ala Lys Ser Ile Asp Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Asn Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Glu Lys Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ala Asp Tyr Asn Lys Lys Ile Thr Ala 
        195                 200                 205             


Glu Phe Ile Asp Asp Tyr Val Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Asn Phe Tyr Pro Asp Glu Tyr Arg Ala Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Tyr Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Ser Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys Glu Ser Leu Val 
    290                 295                 300                 


Glu Phe Ala Lys Asn Thr Ala Thr Leu Gly Pro Ala Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Ile Leu Asp Cys Lys Val Asp Glu Ile Lys Gly Tyr 
                325                 330                 335     


Arg Glu Asp Asp Lys Gly Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Glu Ser Ile Asn Ile Asp Asp Leu Ser 
        355                 360                 365             


Arg Glu Val Ile Asp Lys Leu Ala Asp Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Ala Ile Lys Arg Asn Leu Pro Asn Gln Phe 
385                 390                 395                 400 


Thr Glu Glu Gln Ile Ser Glu Ile Ile Lys Val Arg Lys Ser Gln Ser 
                405                 410                 415     


Thr Ala Phe Asn Lys Gly Trp His Ser Phe Ser Ala Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Ala Thr Ser Asp Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Val Asn Lys Lys Ser Ser Lys Asn 
    450                 455                 460                 


Thr Lys Thr Ile Asp Glu Lys Glu Val Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Thr Ile Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Asp Asp Glu Lys Lys Phe Ile Asp Lys Arg Asn Lys 
        515                 520                 525             


Glu Asn Lys Lys Glu Lys Asp Asp Ala Leu Lys Arg Ala Ala Tyr Leu 
    530                 535                 540                 


Tyr Asn Ser Ser Asp Lys Leu Pro Asp Glu Val Phe His Gly Asn Lys 
545                 550                 555                 560 


Gln Leu Glu Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Pro Ile Ser Ile Gln Glu Leu Val His Asn Ser 
            580                 585                 590         


Asn Asn Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Trp Thr Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Lys Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Ala Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Asp Tyr Val Leu Lys Gln Lys Gly Leu 
                645                 650                 655     


Gly Lys Lys Lys Arg Asp Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Leu Gln Ser Ala Leu Arg Glu 
    690                 695                 700                 


Leu Gly Lys Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Lys 
            740                 745                 750         


Leu Trp Glu Lys Gln Asp Asn Pro Met Phe Val Asp Tyr Gly Lys Asn 
        755                 760                 765             


Gln Val Val Asp Lys Gln Thr Gly Glu Ile Leu Ser Val Ser Asp Asp 
    770                 775                 780                 


Glu Tyr Lys Glu Leu Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Lys Gly Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Tyr Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Ile Gly Lys Asp Lys Lys Glu Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Asn Gly Phe Asp Thr Phe 
    850                 855                 860                 


Ile Lys Lys Tyr Asn Lys Asp Lys Thr Gln Phe Leu Met Tyr Gln Lys 
865                 870                 875                 880 


Asp Ser Leu Thr Trp Glu Asn Val Ile Glu Val Ile Leu Arg Asp Tyr 
                885                 890                 895     


Pro Thr Thr Lys Lys Ser Glu Asp Gly Lys Asn Asp Val Lys Cys Asn 
            900                 905                 910         


Pro Phe Glu Glu Tyr Arg Arg Glu Asn Gly Leu Ile Cys Lys Tyr Ser 
        915                 920                 925             


Lys Lys Gly Lys Gly Thr Pro Ile Lys Ser Leu Lys Tyr Tyr Asp Lys 
    930                 935                 940                 


Lys Leu Gly Asn Cys Ile Asp Ile Thr Pro Glu Glu Ser Arg Asn Lys 
945                 950                 955                 960 


Val Ile Leu Gln Ser Ile Asn Pro Trp Arg Ala Asp Val Tyr Phe Asn 
                965                 970                 975     


Pro Glu Thr Leu Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu 
            980                 985                 990         


Ser Phe Glu Lys Gly Thr Gly Asn  Tyr His Ile Ser Gln  Glu Lys Tyr 
        995                 1000                 1005             


Asp Ala  Ile Lys Glu Lys Glu  Gly Ile Gly Lys Lys  Ser Glu Phe 
    1010                 1015                 1020             


Lys Phe  Thr Leu Tyr Arg Asn  Asp Leu Ile Leu Ile  Lys Asp Ile 
    1025                 1030                 1035             


Ala Ser  Gly Glu Gln Glu Ile  Tyr Arg Phe Leu Ser  Arg Thr Met 
    1040                 1045                 1050             


Pro Asn  Val Asn His Tyr Val  Glu Leu Lys Pro Tyr  Asp Lys Glu 
    1055                 1060                 1065             


Lys Phe  Asp Asn Val Gln Glu  Leu Val Glu Ala Leu  Gly Glu Ala 
    1070                 1075                 1080             


Asp Lys  Val Gly Arg Cys Ile  Lys Gly Leu Asn Lys  Pro Asn Ile 
    1085                 1090                 1095             


Ser Ile  Tyr Lys Val Arg Thr  Asp Val Leu Gly Asn  Lys Tyr Phe 
    1100                 1105                 1110             


Val Lys  Lys Lys Gly Asp Lys  Pro Lys Leu Asp Phe  Lys Asn Asn 
    1115                 1120                 1125             


Lys Lys  
    1130 


<210>  713
<211>  1130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  713

Met Thr Asn Gly Lys Ile Leu Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Val Gly Ile Ile Glu Ala Lys Thr Gly Lys Val Val His Ala Asn 
            20                  25                  30          


Ser Arg Leu Phe Ser Ala Ala Asn Ala Glu Asn Asn Ala Glu Arg Arg 
        35                  40                  45              


Gly Phe Arg Gly Ser Arg Arg Leu Asn Arg Arg Lys Lys His Arg Val 
    50                  55                  60                  


Lys Arg Val Arg Asp Leu Phe Glu Lys Tyr Gly Ile Val Thr Asp Phe 
65                  70                  75                  80  


Arg Asn Leu Asn Leu Asn Pro Tyr Glu Leu Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Asn Glu Glu Leu Phe Ala Ala Leu Arg Thr Ile Ser 
            100                 105                 110         


Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Glu Asp Asp Ser Thr 
        115                 120                 125             


Gly Ser Thr Asp Tyr Ala Lys Ser Ile Asp Glu Asn Arg Arg Leu Leu 
    130                 135                 140                 


Lys Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Leu Glu Lys Tyr 
145                 150                 155                 160 


Gly Gln Leu Arg Gly Asn Phe Thr Val Tyr Asp Glu Asn Gly Glu Ala 
                165                 170                 175     


His Arg Leu Ile Asn Val Phe Ser Thr Ser Asp Tyr Glu Lys Glu Ala 
            180                 185                 190         


Arg Lys Ile Leu Glu Thr Gln Ala Asp Tyr Asn Lys Lys Ile Thr Ala 
        195                 200                 205             


Glu Phe Ile Asp Asp Tyr Val Glu Ile Leu Thr Gln Lys Arg Lys Tyr 
    210                 215                 220                 


Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg Phe 
225                 230                 235                 240 


Arg Thr Asp Gly Thr Thr Leu Glu Asn Ile Phe Gly Ile Leu Ile Gly 
                245                 250                 255     


Lys Cys Ser Phe Tyr Pro Asp Glu Tyr Arg Ala Ser Lys Ala Ser Tyr 
            260                 265                 270         


Thr Ala Gln Glu Tyr Asn Phe Leu Asn Asp Leu Asn Asn Leu Lys Val 
        275                 280                 285             


Pro Thr Glu Thr Gly Lys Leu Ser Thr Glu Gln Lys Glu Ala Leu Val 
    290                 295                 300                 


Glu Phe Ala Lys Ser Thr Ala Thr Leu Gly Pro Ala Lys Leu Leu Lys 
305                 310                 315                 320 


Glu Ile Ala Lys Ile Leu Asp Cys Lys Val Asp Glu Ile Lys Gly Tyr 
                325                 330                 335     


Arg Glu Asp Asp Lys Gly Lys Pro Asp Leu His Thr Phe Glu Pro Tyr 
            340                 345                 350         


Arg Lys Leu Lys Phe Asn Leu Asp Ser Val Asn Ile Asp Tyr Leu Ser 
        355                 360                 365             


Arg Glu Val Leu Asp Lys Leu Ala Gly Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Asp Ala Ile Lys Arg Asn Leu Pro Asn Gln Phe 
385                 390                 395                 400 


Thr Glu Glu Gln Ile Ser Glu Ile Ile Asn Val Arg Lys Ser Gln Ser 
                405                 410                 415     


Thr Ala Phe Asn Lys Gly Trp His Ser Phe Ser Ala Lys Leu Met Asn 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Ala Thr Ser Asp Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Glu Lys Phe Lys Val Lys Lys Lys Ser Ser Lys Asn 
    450                 455                 460                 


Ile Lys Thr Ile Asp Glu Lys Glu Ile Thr Asp Glu Ile Tyr Asn Pro 
465                 470                 475                 480 


Val Val Ala Lys Ser Val Arg Gln Thr Ile Lys Ile Ile Asn Ala Ala 
                485                 490                 495     


Val Lys Lys Tyr Gly Asp Phe Asp Lys Ile Val Ile Glu Met Pro Arg 
            500                 505                 510         


Asp Lys Asn Ala Asp Asp Glu Lys Lys Phe Ile Asn Lys Arg Asn Lys 
        515                 520                 525             


Glu Asn Lys Lys Glu Lys Asp Asp Ala Leu Lys Arg Ala Ala Tyr Leu 
    530                 535                 540                 


Tyr Asn Gly Thr Asp Lys Leu Pro Asp Glu Val Phe His Gly Asn Lys 
545                 550                 555                 560 


Gln Leu Glu Thr Lys Ile Arg Leu Trp Tyr Gln Gln Gly Glu Arg Cys 
                565                 570                 575     


Leu Tyr Ser Gly Lys Pro Ile Pro Ile Gln Glu Leu Val His Asn Ser 
            580                 585                 590         


Asn Asn Phe Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp 
        595                 600                 605             


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ser Trp Thr Asn Gln Glu 
    610                 615                 620                 


Lys Gly Gln Lys Thr Pro Tyr Gln Val Ile Asp Ser Met Asp Ala Ala 
625                 630                 635                 640 


Trp Ser Phe Arg Glu Met Lys Glu Tyr Val Leu Lys Gln Lys Gly Leu 
                645                 650                 655     


Gly Lys Lys Lys Arg Asp Tyr Leu Leu Thr Thr Glu Asn Ile Asp Lys 
            660                 665                 670         


Ile Glu Val Lys Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
        675                 680                 685             


Tyr Ala Ser Arg Val Val Leu Asn Ser Leu Gln Ser Ala Leu Arg Glu 
    690                 695                 700                 


Leu Gly Lys Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
705                 710                 715                 720 


Gln Leu Arg Arg Lys Trp Lys Ile Asp Lys Ser Arg Glu Thr Tyr His 
                725                 730                 735     


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Lys 
            740                 745                 750         


Leu Trp Glu Lys Gln Asp Asn Pro Met Phe Val Asp Tyr Gly Asn Asn 
        755                 760                 765             


Gln Val Val Asp Lys Gln Thr Gly Glu Ile Leu Ser Val Ser Asp Asn 
    770                 775                 780                 


Glu Tyr Lys Glu Leu Val Phe Gln Pro Pro Tyr Gln Gly Phe Val Asn 
785                 790                 795                 800 


Thr Ile Ser Ser Lys Gly Phe Glu Asp Glu Ile Leu Phe Ser Tyr Gln 
                805                 810                 815     


Val Asp Ser Lys Tyr Asn Arg Lys Val Ser Asp Ala Thr Ile Tyr Ser 
            820                 825                 830         


Thr Arg Lys Ala Lys Ile Gly Lys Asp Lys Lys Glu Glu Thr Tyr Val 
        835                 840                 845             


Leu Gly Lys Ile Lys Asp Ile Tyr Ser Gln Asn Gly Phe Asp Thr Phe 
    850                 855                 860                 


Val Lys Lys Tyr Asn Lys Asp Lys Thr Gln Phe Leu Met Tyr Gln Lys 
865                 870                 875                 880 


Asp Pro Leu Thr Trp Glu Asn Val Ile Glu Val Ile Leu Gly Asp Tyr 
                885                 890                 895     


Pro Thr Thr Lys Lys Ser Glu Asp Gly Lys Asn Asp Val Lys Cys Asn 
            900                 905                 910         


Pro Phe Glu Glu Tyr Arg Arg Glu Asn Gly Leu Ile Cys Lys Tyr Ser 
        915                 920                 925             


Lys Lys Gly Lys Gly Thr Pro Ile Lys Ser Leu Lys Tyr Tyr Asp Lys 
    930                 935                 940                 


Lys Leu Gly Asn Cys Ile Asp Ile Thr Pro Glu Glu Ser Arg Asn Lys 
945                 950                 955                 960 


Val Ile Leu Gln Ser Ile Asn Pro Trp Arg Ala Asp Val Tyr Phe Asn 
                965                 970                 975     


Pro Glu Thr Leu Lys Tyr Glu Leu Met Gly Leu Lys Tyr Ser Asp Leu 
            980                 985                 990         


Ser Phe Glu Lys Gly Thr Gly Asn  Tyr His Ile Ser Gln  Glu Lys Tyr 
        995                 1000                 1005             


Asp Ala  Ile Lys Glu Lys Glu  Gly Ile Gly Lys Lys  Ser Glu Phe 
    1010                 1015                 1020             


Lys Phe  Thr Leu Tyr Arg Asn  Asp Leu Ile Leu Ile  Lys Asp Ile 
    1025                 1030                 1035             


Ala Ser  Gly Glu Gln Glu Ile  Tyr Arg Phe Leu Ser  Arg Thr Met 
    1040                 1045                 1050             


Pro Asn  Val Asn His Tyr Val  Glu Leu Lys Pro Tyr  Asp Lys Glu 
    1055                 1060                 1065             


Lys Phe  Asp Asn Val Gln Glu  Leu Val Glu Ala Leu  Gly Glu Ala 
    1070                 1075                 1080             


Asp Lys  Val Gly Arg Cys Ile  Lys Gly Leu Asn Lys  Pro Asn Ile 
    1085                 1090                 1095             


Ser Ile  Tyr Lys Val Arg Thr  Asp Val Leu Gly Asn  Lys Tyr Phe 
    1100                 1105                 1110             


Val Lys  Lys Glu Gly Asp Lys  Pro Lys Leu Asp Phe  Lys Asn Asn 
    1115                 1120                 1125             


Lys Lys  
    1130 


<210>  714
<211>  1131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  714

Met Ala Gln His Val Phe Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Trp Ala Ile Leu Gly Glu Gln Arg Ile Ile Asp Leu Gly Val Arg Cys 
            20                  25                  30          


Phe Asp Lys Ala Glu Thr Ala Lys Glu Gly Asp Pro Leu Asn Leu Thr 
        35                  40                  45              


Arg Arg Gln Ala Arg Leu Leu Arg Arg Arg Leu Tyr Arg Arg Ala Trp 
    50                  55                  60                  


Arg Leu Thr Gln Leu Ser Arg Leu Leu Lys Arg Lys Gly Leu Ile Ala 
65                  70                  75                  80  


Asp Ala Lys Leu Phe Ala Lys Ala Pro Ser Tyr Gly Asp Ser Ala Trp 
                85                  90                  95      


Glu Leu Arg Arg Gln Gly Leu Asp Arg Leu Leu Thr Pro Leu Glu Trp 
            100                 105                 110         


Ala Arg Val Ile Tyr His Gln Cys Lys His Arg Gly Phe His Trp Thr 
        115                 120                 125             


Ser Lys Ala Glu Glu Ala Lys Ala Asp Ser Asp Ala Glu Gly Gly Arg 
    130                 135                 140                 


Val Lys Gln Gly Leu Ala His Thr Lys Ala Leu Met Gln Ala Lys Asn 
145                 150                 155                 160 


Tyr Arg Ser Ala Ala Glu Met Val Leu Ala Glu Phe Pro Asp Ala Gln 
                165                 170                 175     


Arg Asn Lys Arg Gly Gln Tyr Asp Lys Ala Leu Ser Arg Val Leu Leu 
            180                 185                 190         


Gly Glu Glu Leu Ala Leu Leu Phe Ala Thr Gln Arg Arg Leu Gly Asn 
        195                 200                 205             


Pro His Ala Ser Asp Phe Phe Glu Lys Leu Ile Leu Gly Asp Gly Asp 
    210                 215                 220                 


Arg Lys Ser Gly Leu Phe Trp Gln Gln Lys Pro Ala Leu Ser Gly Ala 
225                 230                 235                 240 


Asp Leu Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Lys Gly Glu Tyr 
                245                 250                 255     


Arg Ala Pro Lys Ala Ser Phe Ser Val Glu Arg His Val Trp Leu Thr 
            260                 265                 270         


Arg Leu Asn Asn Leu Arg Ile Val Val Asp Gly Arg Ser Arg Pro Leu 
        275                 280                 285             


Asn Glu Ala Glu Arg Gln Ala Ala Leu Leu Leu Pro Tyr Gln Thr Glu 
    290                 295                 300                 


Thr Ser Lys Tyr Lys Thr Leu Lys Asn Ala Phe Ile Lys Ala Gly Leu 
305                 310                 315                 320 


Trp Gly Asp Gly Val Arg Phe Gly Gly Leu Ala Tyr Pro Ser Gln Ala 
                325                 330                 335     


Gln Ile Asp Ala Glu Lys Thr Lys Asp Pro Glu Asp Gln Phe Leu Val 
            340                 345                 350         


Lys Leu Pro Ala Trp His Glu Leu Arg Lys Ala Phe Lys Ala Ala Gly 
        355                 360                 365             


His Glu Ala Leu Trp Gln Gln Ile Ser Thr Pro Ala Leu Asp Gly Asp 
    370                 375                 380                 


Pro Thr Leu Leu Asp Gln Ile Ala Thr Val Leu Ser Val Tyr Lys Asp 
385                 390                 395                 400 


Gly Ala Glu Val Val Gln Gln Leu Arg Gln Leu Ala Leu Pro Glu Pro 
                405                 410                 415     


Ala Ala Ser Ile Ala Val Leu Glu Lys Ile Ser Phe Asp Lys Phe Ser 
            420                 425                 430         


Ser Leu Ser Leu Lys Ala Leu Arg Arg Ile Val Pro Leu Met Gln Ser 
        435                 440                 445             


Gly Leu Arg Tyr Asp Glu Ala Val Ala Gln Ile Pro Glu Tyr Gly His 
    450                 455                 460                 


His Ser Gln Arg Ile Glu Pro Gly Ala Ala Lys His Leu Tyr Leu Pro 
465                 470                 475                 480 


Pro Phe Tyr Glu Ala Gln Arg Lys Tyr Ala Gly Lys Gly Asp His Ile 
                485                 490                 495     


Gly Ser Met Gln Phe Arg Asp Asp Ala Asp Ile Pro Arg Asn Pro Val 
            500                 505                 510         


Val Leu Arg Ala Leu Asn Gln Ala Arg Lys Val Val Asn Ala Leu Ile 
        515                 520                 525             


Arg Glu Tyr Gly Ser Pro Ile Ala Val Asn Ile Glu Met Ala Arg Asp 
    530                 535                 540                 


Leu Ser Arg Pro Leu Asp Glu Arg Asn Lys Val Lys Arg Ala Gln Glu 
545                 550                 555                 560 


Glu Phe Arg Asp Arg Asn Asp Arg Ala Arg Ser Glu Phe Glu Arg Asp 
                565                 570                 575     


Phe Gly Tyr Lys Pro Lys Ala Ala Ala Phe Glu Lys Trp Met Leu Tyr 
            580                 585                 590         


Arg Glu Gln Leu Gly Gln Cys Ala Tyr Ser Gln Gln Pro Leu Asp Ile 
        595                 600                 605             


Gln Arg Val Leu Asp Asp His Asn Tyr Ala Gln Val Asp His Ala Leu 
    610                 615                 620                 


Pro Tyr Ser Arg Ser Tyr Asp Asp Ser Lys Asn Asn Lys Val Leu Val 
625                 630                 635                 640 


Leu Thr His Glu Asn Gln Asn Lys Gly Asn Arg Thr Ala Phe Glu Tyr 
                645                 650                 655     


Leu Thr Ser Phe Pro Asp Gly Glu Asp Gly Glu Arg Trp Arg Thr Phe 
            660                 665                 670         


Val Ala Trp Val Gln Gly Asn Lys Ala Tyr Arg Met Ala Lys Arg Asn 
        675                 680                 685             


Arg Leu Leu Arg Lys Asn Tyr Gly Val Asp Glu Ser Lys Gly Phe Ile 
    690                 695                 700                 


Asp Arg Asn Leu Asn Asp Thr Arg Tyr Ile Cys Lys Phe Phe Lys Asn 
705                 710                 715                 720 


Tyr Val Glu Glu His Leu Gln Leu Ala Ala Arg Ala Asp Gly Asp Thr 
                725                 730                 735     


Ala Arg Arg Cys Val Val Val Asn Gly Gln Leu Thr Ala Phe Leu Arg 
            740                 745                 750         


Ala Arg Trp Gly Leu Thr Lys Val Arg Gly Asp Ser Asp Arg His His 
        755                 760                 765             


Ala Leu Asp Ala Ala Val Val Ala Ala Cys Thr His Gly Met Val Lys 
    770                 775                 780                 


Ala Leu Ala Asp Tyr Ser Arg Arg Lys Glu Ile Ser Phe Leu Gln Glu 
785                 790                 795                 800 


Gly Phe Pro Asp Pro Glu Thr Gly Glu Ile Leu Asn Pro Ala Ala Phe 
                805                 810                 815     


Asp Arg Ala Arg Gln His Phe Pro Glu Pro Trp Thr His Phe Ala His 
            820                 825                 830         


Glu Leu Lys Ala Arg Leu Phe Thr Asp Asp Leu Ala Ala Leu Arg Glu 
        835                 840                 845             


Asp Met Gln Arg Leu Gly Ser Tyr Thr Thr Glu Asp Leu Gly Arg Leu 
    850                 855                 860                 


Arg Thr Leu Phe Val Ser Arg Ala Pro Gln Arg Arg Ser Gly Gly Ala 
865                 870                 875                 880 


Val His Lys Glu Thr Ile Tyr Ala Gln Pro Glu Ser Leu Lys Gln Gln 
                885                 890                 895     


Gly Gly Val Ile Glu Lys Ile Leu Leu Thr Ser Leu Lys Leu Gln Asp 
            900                 905                 910         


Phe Asp Lys Leu Leu Asn Pro Glu Ser Asn Asp His Phe Val Glu Pro 
        915                 920                 925             


His Arg Asn Glu Arg Leu Tyr Ala Ala Ile Arg Gln Arg Leu Glu Gln 
    930                 935                 940                 


Phe Gly Gly Arg Ala Asp Lys Ala Phe Gly Pro Asp Asn Leu Phe His 
945                 950                 955                 960 


Lys Pro Asp Lys Asn Asn Gln Pro Thr Gly Pro Val Val Arg Ser Ile 
                965                 970                 975     


Lys Leu Val Arg Gly Lys Gln Thr Gly Ile Pro Ile Arg Gly Gly Leu 
            980                 985                 990         


Ala Lys Asn Asp Ser Met Leu Arg  Val Asp Ile Phe Thr  Lys Ala Gly 
        995                 1000                 1005             


Lys Phe  His Leu Val Pro Val  Tyr Val His His Arg  Val Thr Gly 
    1010                 1015                 1020             


Leu Pro  Asn Arg Ala Ile Val  Ala Phe Lys Asp Glu  Asp Glu Trp 
    1025                 1030                 1035             


Thr Leu  Ile Asp Glu Ser Phe  Ala Phe Leu Phe Ser  Val Tyr Pro 
    1040                 1045                 1050             


Asn Asp  Tyr Val Lys Val Thr  Leu Lys Lys Glu Gln  Gln Ser Gly 
    1055                 1060                 1065             


Tyr Tyr  Ser Gly Ala Asp Arg  Ser Thr Gly Ala Met  Asn Leu Trp 
    1070                 1075                 1080             


Ala His  Asp Arg Ala Ala Ser  Val Gly Lys Asp Gly  Leu Ile Arg 
    1085                 1090                 1095             


Gly Ile  Gly Val Lys Thr Ala  Leu Ser Val Glu Lys  Phe Asn Val 
    1100                 1105                 1110             


Asp Val  Leu Gly Arg Ile Tyr  Leu Ala Pro Pro Glu  Thr Arg Ser 
    1115                 1120                 1125             


Gly Leu  Ala 
    1130     


<210>  715
<211>  1132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  715

Met Ala Gln Tyr Val Phe Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Leu Asp Ala Gln Arg Ile Val Asp Leu Gly Val Arg Cys 
            20                  25                  30          


Phe Asp Lys Ala Glu Thr Ala Lys Glu Gly Asp Pro Leu Asn Leu Thr 
        35                  40                  45              


Arg Arg Gln Ala Arg Leu Leu Arg Arg Arg Leu Tyr Arg Arg Ala Trp 
    50                  55                  60                  


Arg Leu Thr Gln Leu Ala Arg Leu Phe Lys Arg Glu Gly Leu Ile Asp 
65                  70                  75                  80  


Asp Ala Lys Leu Phe Ala Lys Ala Pro Ser Trp Thr Glu Asn Ala Trp 
                85                  90                  95      


Glu Leu Arg Leu Arg Gly Leu Asp His Leu Leu Thr Pro Leu Glu Trp 
            100                 105                 110         


Ala Arg Val Ile Tyr His Leu Cys Lys His Arg Gly Phe His Trp Thr 
        115                 120                 125             


Ser Lys Ala Glu Glu Ala Lys Ala Glu Ser Asp Ala Glu Gly Gly Arg 
    130                 135                 140                 


Val Lys Gln Gly Leu Ala His Thr Lys Ala Leu Met Gln Val Lys Asn 
145                 150                 155                 160 


Tyr Arg Ser Thr Ala Glu Met Val Leu Ala Glu Phe Pro Gly Ala Gln 
                165                 170                 175     


Arg Asn Lys Arg Gly Gln Tyr Asp Lys Ala Leu Ser Arg Val Leu Leu 
            180                 185                 190         


Gly Glu Glu Leu Ala Leu Leu Phe Ala Thr Gln Arg Arg Leu Gly Asn 
        195                 200                 205             


Pro His Ala Ser Glu His Phe Glu Gln Leu Ile Leu Gly His Gly Asp 
    210                 215                 220                 


Arg Lys Ser Gly Leu Phe Trp Gln Gln Lys Pro Ala Leu Ser Gly Ala 
225                 230                 235                 240 


Asp Leu Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Lys Thr Glu Tyr 
                245                 250                 255     


Arg Ala Pro Lys Ala Ser Phe Ser Ala Glu Arg His Val Trp Leu Thr 
            260                 265                 270         


Arg Leu Asn Asn Leu Arg Ile Val Val Asp Gly Arg Ala Arg Pro Leu 
        275                 280                 285             


Asn Asp Ala Glu Arg Gln Ala Ala Leu Leu Leu Pro Tyr Gln Thr Glu 
    290                 295                 300                 


Thr Phe Lys Tyr Lys Thr Leu Lys Ala Ala Phe Val Lys Val Gly Leu 
305                 310                 315                 320 


Trp Gly Asp Gly Val Arg Phe Gly Gly Leu Ala Tyr Pro Ser Gln Ala 
                325                 330                 335     


Gln Ala Glu Ala Glu Lys Ala Lys Asp Pro Glu Asp Gln Phe Leu Val 
            340                 345                 350         


Lys Leu Pro Ala Trp His Glu Leu Arg Lys Ala Phe Lys Ala Ala Gly 
        355                 360                 365             


His Glu Ala Leu Trp Gln Gln Ile Ser Met Pro Ala Leu Glu Glu Asp 
    370                 375                 380                 


Pro Thr Leu Leu Asp Gln Ile Ala Thr Val Leu Ser Val Tyr Lys Asp 
385                 390                 395                 400 


Gly Thr Glu Val Val Gln Gln Leu Arg Gln Leu Ala Leu Pro Glu Pro 
                405                 410                 415     


Asp Ala Ser Ile Ala Val Leu Glu Lys Ile Arg Phe Asp Lys Phe Ser 
            420                 425                 430         


Ser Leu Ser Leu Lys Ala Leu Arg Arg Ile Val Pro Leu Met Gln Gly 
        435                 440                 445             


Gly Leu Arg Tyr Asp Glu Ala Val Ala Gln Ile Pro Glu Tyr Gly His 
    450                 455                 460                 


His Ser Gln Arg Ile Asp Pro Gly Ala Thr Arg His Leu Tyr Leu Pro 
465                 470                 475                 480 


Pro Phe Tyr Glu Ala Gln Arg Lys Tyr Ala Gly Lys Gly Asp Arg Ile 
                485                 490                 495     


Gly Ser Met Gln Phe Arg Glu Asp Ala Asp Val Pro Arg Asn Pro Val 
            500                 505                 510         


Val Leu Arg Ala Leu Asn Gln Ala Arg Lys Val Val Asn Ala Leu Ile 
        515                 520                 525             


Arg Glu Tyr Gly Ser Pro Val Ala Val Asn Ile Glu Met Ala Arg Asp 
    530                 535                 540                 


Leu Ser Arg Pro Leu Asp Glu Arg Asn Lys Val Lys Arg Ala Gln Glu 
545                 550                 555                 560 


Glu Phe Arg Asp Arg Asn Asp Lys Ala Arg Ser Glu Phe Glu Arg Asp 
                565                 570                 575     


Phe Gly Tyr Lys Pro Lys Gly Ala Val Phe Glu Lys Trp Met Leu Tyr 
            580                 585                 590         


Arg Glu Gln Leu Gly Gln Cys Ala Tyr Ser Gln Gln Pro Leu Asp Ile 
        595                 600                 605             


Gln Arg Val Leu Asp Asp Pro Asn Tyr Ala Gln Val Asp His Ala Leu 
    610                 615                 620                 


Pro Tyr Ser Arg Ser Tyr Asp Asp Ser Lys Asn Asn Lys Val Leu Val 
625                 630                 635                 640 


Leu Thr Arg Glu Asn Gln Asn Lys Gly Asn Arg Thr Pro Phe Glu Tyr 
                645                 650                 655     


Leu Thr Ser Phe Pro Gly Gly Glu Asp Gly Glu Arg Trp Arg Ala Phe 
            660                 665                 670         


Val Ala Trp Val Gln Gly Asn Lys Ala Tyr Arg Met Ala Lys Arg Asn 
        675                 680                 685             


Arg Leu Leu Arg Lys Asn Tyr Gly Thr Asp Glu Ser Arg Gly Phe Ile 
    690                 695                 700                 


Asp Arg Asn Leu Asn Asp Thr Arg Tyr Ile Cys Lys Phe Phe Lys Asn 
705                 710                 715                 720 


Tyr Val Glu Glu His Leu Gln Leu Ala Ala Arg Thr Asp Gly Asp Thr 
                725                 730                 735     


Ala Arg Arg Cys Val Val Val Asn Gly Gln Leu Thr Ala Phe Leu Arg 
            740                 745                 750         


Ala Arg Trp Gly Leu Ala Lys Val Arg Gly Asp Ser Asp Arg His His 
        755                 760                 765             


Ala Leu Asp Ala Ala Val Val Ala Ala Cys Thr His Gly Met Val Lys 
    770                 775                 780                 


Ala Leu Ala Asp Tyr Ser Arg Arg Lys Glu Ile Ala Phe Leu Gln Glu 
785                 790                 795                 800 


Gly Phe Pro Asp Pro Glu Thr Gly Glu Ile Leu Asn Leu Ala Ala Phe 
                805                 810                 815     


Asp Arg Ala Arg Glu His Phe Pro Glu Pro Trp Pro His Phe Ala His 
            820                 825                 830         


Glu Leu Lys Ala Arg Leu Phe Ile Asp Asp Leu Val Ala Leu Arg Glu 
        835                 840                 845             


Asp Met Gln His Leu Gly Ser Tyr Ala Gln Asp Asp Leu Asp Arg Leu 
    850                 855                 860                 


Arg Thr Leu Phe Val Ser Arg Ala Pro Gln Arg Arg Asn Gly Gly Ala 
865                 870                 875                 880 


Val His Lys Gly Thr Ile Tyr Ala Gln Pro Glu Asn Leu Lys Gln Gln 
                885                 890                 895     


Gly Ser Thr Ile Glu Lys Ile Leu Leu Thr Gly Leu Lys Leu Gln Asp 
            900                 905                 910         


Phe Asp Lys Leu Leu Asn Pro Glu Ser Ser Asp His Phe Val Glu Pro 
        915                 920                 925             


His Arg Asn Glu Arg Leu Tyr Ala Ala Ile Arg Arg Arg Leu Glu Glu 
    930                 935                 940                 


Phe Gly Gly Lys Ala Asp Lys Ala Phe Gly Pro Asp Lys Pro Phe Tyr 
945                 950                 955                 960 


Lys Pro Asp Lys Asn Gly Gln Pro Thr Gly Pro Ile Val Arg Ser Ile 
                965                 970                 975     


Lys Leu Val Arg Gly Lys Gln Thr Gly Ile Pro Ile Arg Gly Gly Leu 
            980                 985                 990         


Ala Lys Asn Asp Ser Met Leu Arg  Val Asp Val Phe Thr  Lys Ala Gly 
        995                 1000                 1005             


Lys Phe  His Leu Val Pro Val  Tyr Val His His Arg  Val Thr Gly 
    1010                 1015                 1020             


Leu Pro  Asp Arg Ala Ile Val  Ala Phe Lys Asp Glu  Glu Glu Trp 
    1025                 1030                 1035             


Thr Leu  Ile Asp Glu Ser Tyr  Ala Phe Leu Phe Ser  Val Tyr Pro 
    1040                 1045                 1050             


Asn Asp  Phe Val Cys Val Gln  Gln Arg Gly Lys Pro  Leu Ile Glu 
    1055                 1060                 1065             


Gly Tyr  Tyr Ala Gly Leu Asp  Arg Ala Asn Gly Asn  Ile Asn Val 
    1070                 1075                 1080             


Trp Ala  His Asp Arg Ala Ala  Ser Val Gly Lys Asp  Gly Leu Ile 
    1085                 1090                 1095             


Arg Gly  Ile Gly Val Lys Thr  Ala Leu Ser Leu Glu  Lys Leu Asn 
    1100                 1105                 1110             


Val Asp  Val Leu Gly Arg Ile  Tyr Pro Ala Pro Pro  Glu Thr Arg 
    1115                 1120                 1125             


Arg Gly  Leu Ala 
    1130         


<210>  716
<211>  1132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  716

Met Lys Lys Ile Leu Gly Val Asp Leu Gly Ile Thr Ser Phe Gly Tyr 
1               5                   10                  15      


Ala Ile Leu Gln Glu Thr Gly Lys Asp Leu Tyr Arg Cys Leu Asp Asn 
            20                  25                  30          


Ser Val Val Met Arg Asn Asn Pro Tyr Asp Glu Lys Ser Gly Glu Ser 
        35                  40                  45              


Ser Gln Ser Ile Arg Ser Thr Gln Lys Ser Met Arg Arg Leu Ile Glu 
    50                  55                  60                  


Lys Arg Lys Lys Arg Ile Arg Cys Val Ala Gln Thr Met Glu Arg Tyr 
65                  70                  75                  80  


Gly Ile Leu Asp Tyr Ser Glu Thr Met Lys Ile Asn Asp Pro Lys Asn 
                85                  90                  95      


Asn Pro Ile Lys Asn Arg Trp Gln Leu Arg Ala Val Asp Ala Trp Lys 
            100                 105                 110         


Arg Pro Leu Ser Pro Gln Glu Leu Phe Ala Ile Phe Ala His Met Ala 
        115                 120                 125             


Lys His Arg Gly Tyr Lys Ser Ile Ala Thr Glu Asp Leu Ile Tyr Glu 
    130                 135                 140                 


Leu Glu Leu Glu Leu Gly Leu Asn Asp Pro Glu Lys Glu Ser Glu Lys 
145                 150                 155                 160 


Lys Ala Asp Glu Arg Arg Gln Val Tyr Asn Ala Leu Arg His Leu Glu 
                165                 170                 175     


Glu Leu Arg Lys Lys Tyr Gly Gly Glu Thr Ile Ala Gln Thr Ile His 
            180                 185                 190         


Arg Ala Val Glu Ala Gly Asp Leu Arg Ser Tyr Arg Asn His Asp Asp 
        195                 200                 205             


Tyr Glu Lys Met Ile Arg Arg Glu Asp Ile Glu Glu Glu Ile Glu Lys 
    210                 215                 220                 


Val Leu Leu Arg Gln Ala Glu Leu Gly Ala Leu Gly Leu Pro Glu Glu 
225                 230                 235                 240 


Gln Val Ser Glu Leu Ile Asp Glu Leu Lys Ala Cys Ile Thr Asp Gln 
                245                 250                 255     


Glu Met Pro Thr Ile Asp Glu Ser Leu Phe Gly Lys Cys Thr Phe Tyr 
            260                 265                 270         


Lys Asp Glu Leu Ala Ala Pro Ala Tyr Ser Tyr Leu Tyr Asp Leu Tyr 
        275                 280                 285             


Arg Leu Tyr Lys Lys Leu Ala Asp Leu Asn Ile Asp Gly Tyr Glu Val 
    290                 295                 300                 


Thr Gln Glu Asp Arg Glu Lys Val Ile Glu Trp Val Glu Lys Lys Ile 
305                 310                 315                 320 


Ala Gln Gly Lys Asn Leu Lys Lys Ile Thr His Lys Asp Leu Arg Lys 
                325                 330                 335     


Ile Leu Gly Leu Ala Pro Glu Gln Lys Ile Phe Gly Val Glu Asp Glu 
            340                 345                 350         


Arg Ile Val Lys Gly Lys Lys Glu Pro Arg Thr Phe Val Pro Phe Phe 
        355                 360                 365             


Phe Leu Ala Asp Ile Ala Lys Phe Lys Glu Leu Phe Ala Ser Ile Gln 
    370                 375                 380                 


Lys His Pro Asp Ala Leu Gln Ile Phe Arg Glu Leu Ala Glu Ile Leu 
385                 390                 395                 400 


Gln Arg Ser Lys Thr Pro Gln Glu Ala Leu Asp Arg Leu Arg Ala Leu 
                405                 410                 415     


Met Ala Gly Lys Gly Ile Asp Thr Asp Asp Arg Glu Leu Leu Glu Leu 
            420                 425                 430         


Phe Lys Asn Lys Arg Ser Gly Thr Arg Glu Leu Ser His Arg Tyr Ile 
        435                 440                 445             


Leu Glu Ala Leu Pro Leu Phe Leu Glu Gly Tyr Asp Glu Lys Glu Val 
    450                 455                 460                 


Gln Arg Ile Leu Gly Phe Asp Asp Arg Glu Asp Tyr Ser Arg Tyr Pro 
465                 470                 475                 480 


Lys Ser Leu Arg His Leu His Leu Arg Glu Gly Asn Leu Phe Glu Lys 
                485                 490                 495     


Glu Glu Asn Pro Ile Asn Asn His Ala Val Lys Ser Leu Ala Ser Trp 
            500                 505                 510         


Ala Leu Gly Leu Ile Ala Asp Leu Ser Trp Arg Tyr Gly Pro Phe Asp 
        515                 520                 525             


Glu Ile Ile Leu Glu Thr Thr Arg Asp Ala Leu Pro Glu Lys Ile Arg 
    530                 535                 540                 


Lys Glu Ile Asp Lys Ala Met Arg Glu Arg Glu Lys Ala Leu Asp Lys 
545                 550                 555                 560 


Ile Ile Gly Lys Tyr Lys Lys Glu Phe Pro Ser Ile Asp Lys Arg Leu 
                565                 570                 575     


Ala Arg Lys Ile Gln Leu Trp Glu Arg Gln Lys Gly Leu Asp Leu Tyr 
            580                 585                 590         


Ser Gly Lys Val Ile Asn Leu Ser Gln Leu Leu Asp Gly Ser Ala Asp 
        595                 600                 605             


Ile Glu His Ile Val Pro Gln Ser Leu Gly Gly Leu Ser Thr Asp Tyr 
    610                 615                 620                 


Asn Thr Ile Val Thr Leu Lys Ser Val Asn Ala Ala Lys Gly Asn Arg 
625                 630                 635                 640 


Leu Pro Gly Asp Trp Leu Ala Gly Asn Pro Asp Tyr Arg Glu Arg Ile 
                645                 650                 655     


Gly Met Leu Ser Glu Lys Gly Leu Ile Asp Trp Lys Lys Arg Lys Asn 
            660                 665                 670         


Leu Leu Ala Gln Ser Leu Asp Glu Ile Tyr Thr Glu Asn Thr His Ser 
        675                 680                 685             


Lys Gly Ile Arg Ala Thr Ser Tyr Leu Glu Ala Leu Val Ala Gln Val 
    690                 695                 700                 


Leu Lys Arg Tyr Tyr Pro Phe Pro Asp Pro Glu Leu Arg Lys Asn Gly 
705                 710                 715                 720 


Ile Gly Val Arg Met Ile Pro Gly Lys Val Thr Ser Lys Thr Arg Ser 
                725                 730                 735     


Leu Leu Gly Ile Lys Ser Lys Ser Arg Glu Thr Asn Phe His His Ala 
            740                 745                 750         


Glu Asp Ala Leu Ile Leu Ser Thr Leu Thr Arg Gly Trp Gln Asn Arg 
        755                 760                 765             


Leu His Arg Met Leu Arg Asp Asn Tyr Gly Lys Ser Glu Ala Glu Leu 
    770                 775                 780                 


Lys Glu Leu Trp Lys Lys Tyr Met Pro His Ile Glu Gly Leu Thr Leu 
785                 790                 795                 800 


Ala Asp Tyr Ile Asp Glu Ala Phe Arg Arg Phe Met Ser Lys Gly Glu 
                805                 810                 815     


Glu Ser Leu Phe Tyr Arg Asp Met Phe Asp Thr Ile Arg Ser Ile Ser 
            820                 825                 830         


Tyr Trp Val Asp Lys Lys Pro Leu Ser Ala Ser Ser His Lys Glu Thr 
        835                 840                 845             


Val Tyr Ser Ser Arg His Glu Val Pro Thr Leu Arg Lys Asn Ile Leu 
    850                 855                 860                 


Glu Ala Phe Asp Ser Leu Asn Val Ile Lys Asp Arg His Lys Leu Thr 
865                 870                 875                 880 


Thr Glu Glu Phe Met Lys Arg Tyr Asp Lys Glu Ile Arg Gln Lys Leu 
                885                 890                 895     


Trp Leu His Arg Ile Gly Asn Thr Asn Asp Glu Ser Tyr Arg Ala Val 
            900                 905                 910         


Glu Glu Arg Ala Thr Gln Ile Ala Gln Ile Leu Thr Arg Tyr Gln Leu 
        915                 920                 925             


Met Asp Ala Gln Asn Asp Lys Glu Ile Asp Glu Lys Phe Gln Gln Ala 
    930                 935                 940                 


Leu Lys Glu Leu Ile Thr Ser Pro Ile Glu Val Thr Gly Lys Leu Leu 
945                 950                 955                 960 


Arg Lys Met Arg Phe Val Tyr Asp Lys Leu Asn Ala Met Gln Ile Asp 
                965                 970                 975     


Arg Gly Leu Val Glu Thr Asp Lys Asn Met Leu Gly Ile His Ile Ser 
            980                 985                 990         


Lys Gly Pro Asn Glu Lys Leu Ile  Phe Arg Arg Met Asp  Val Asn Asn 
        995                 1000                 1005             


Ala His  Glu Leu Gln Lys Glu  Arg Ser Gly Ile Leu  Cys Tyr Leu 
    1010                 1015                 1020             


Asn Glu  Met Leu Phe Ile Phe  Asn Lys Lys Gly Leu  Ile His Tyr 
    1025                 1030                 1035             


Gly Cys  Leu Arg Ser Tyr Leu  Glu Lys Gly Gln Gly  Ser Lys Tyr 
    1040                 1045                 1050             


Ile Ala  Leu Phe Asn Pro Arg  Phe Pro Ala Asn Pro  Lys Ala Gln 
    1055                 1060                 1065             


Pro Ser  Lys Phe Thr Ser Asp  Ser Lys Ile Lys Gln  Val Gly Ile 
    1070                 1075                 1080             


Gly Ser  Ala Thr Gly Ile Ile  Lys Ala His Leu Asp  Leu Asp Gly 
    1085                 1090                 1095             


His Val  Arg Ser Tyr Glu Val  Phe Gly Thr Leu Pro  Glu Gly Ser 
    1100                 1105                 1110             


Ile Glu  Trp Phe Lys Glu Glu  Ser Gly Tyr Gly Arg  Val Glu Asp 
    1115                 1120                 1125             


Asp Pro  His His 
    1130         


<210>  717
<211>  1133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  717

Met Asp Leu Asn Tyr Arg Ile Gly Leu Asp Ile Gly Ile Ala Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Glu Asn Asn Ser Lys Asp Glu Pro Val Arg Ile 
            20                  25                  30          


Val Asp Leu Gly Val Arg Leu Phe Glu Ala Ala Glu Val Pro Lys Thr 
        35                  40                  45              


Gly Ala Ala Leu Ala Glu Pro Arg Arg Glu Ala Arg Thr Thr Arg Arg 
    50                  55                  60                  


Arg Leu Arg Arg Arg Lys His Arg Leu Glu Arg Met Lys Trp Leu Leu 
65                  70                  75                  80  


Gln Glu Tyr Gly Leu Ile Gln Thr Glu Glu Phe Met Lys Arg Tyr His 
                85                  90                  95      


Ser Ala Gly Leu Pro Asp Val Tyr Arg Leu Arg Tyr Glu Gly Leu Glu 
            100                 105                 110         


Arg Lys Leu Lys Asp Glu Glu Leu Ala Gln Ile Leu Ile His Ile Gly 
        115                 120                 125             


Lys His Arg Gly Phe Arg Ser Thr Arg Lys Ala Glu Leu Lys Asp Lys 
    130                 135                 140                 


Asn Asp Lys Glu Thr Gly Ala Val Leu Ser Ala Thr Arg Glu Asn Glu 
145                 150                 155                 160 


Lys Leu Met Glu Glu His Gly Tyr Arg Thr Ala Gly Glu Met Ile Tyr 
                165                 170                 175     


Lys Asp Asp Lys Phe Arg Thr Gly Cys Thr Trp Lys Glu Glu Gly Tyr 
            180                 185                 190         


Arg Leu Thr Pro Arg Asn Lys Glu Gly Asp Tyr Lys Asn Thr Met Leu 
        195                 200                 205             


Arg Ala Leu Leu Glu Asp Glu Val Lys Val Ile Phe Asp Cys Gln Arg 
    210                 215                 220                 


Lys Phe Gly Asn Gln Lys Ala Ser Glu Glu Leu Glu Lys Ala Tyr Leu 
225                 230                 235                 240 


Ala Ile Met Thr Ser Gln Arg Ser Phe Asp Met Gly Pro Gly Lys Gln 
                245                 250                 255     


Pro Asp Gly Ser Ala Ser Pro Tyr Ala Met Glu Gly Phe Gly Asp Arg 
            260                 265                 270         


Val Gly Leu Cys Ala Leu Glu Pro Arg Glu Lys Gln Glu Pro Arg Ala 
        275                 280                 285             


Pro Lys Ala Ala Tyr Thr Ser Glu Leu Phe Val Ala Leu Gln Lys Ile 
    290                 295                 300                 


Asn His Leu Arg Ile Thr Glu Lys Asn Gly Lys Gly Arg Gly Leu Thr 
305                 310                 315                 320 


Ser Glu Glu Arg Glu Arg Leu Ser Asp Leu Leu His Lys Lys Lys Glu 
                325                 330                 335     


Leu Thr Tyr Gly Ala Val Arg Lys Glu Leu Gln Leu Asp Leu Arg Tyr 
            340                 345                 350         


Arg Phe Asn Thr Leu Asn Tyr Ser Ser Lys Lys Met Thr Glu Glu Glu 
        355                 360                 365             


Lys Ile Lys Asp Ala Glu Lys Ala Lys Phe Val Ser Met Val Ser His 
    370                 375                 380                 


Tyr Glu Tyr Arg Lys Arg Met Ser Gly Met Leu Glu Gln Leu Ser Pro 
385                 390                 395                 400 


Glu Glu Gln Ala Asp Leu Met Asp Glu Val Gly Lys Ile Leu Thr Leu 
                405                 410                 415     


Tyr Lys Asn Asp Asp Thr Arg Ser Arg Glu Phe Gln Lys Leu Gly Leu 
            420                 425                 430         


Asp Ser Glu Ile Thr Glu Glu Leu Leu Glu Leu Asn Pro Arg Gly Phe 
        435                 440                 445             


Gln His Val Ser Leu Lys Ala Met Lys Lys Leu Met Pro Tyr Leu Lys 
    450                 455                 460                 


Glu Gly Leu Thr Tyr Asp Lys Ala Cys Ala Glu Ala Gly Tyr Asp Phe 
465                 470                 475                 480 


Gln Gly Ser Val Gln Lys Glu Arg Ser Arg Leu Leu Lys Gly Lys Glu 
                485                 490                 495     


Ile Thr Asp Ile Ile Asn Asp Ile Asn Asn Pro Val Val Lys Arg Ser 
            500                 505                 510         


Val Ser Gln Thr Val Lys Val Ile Asn Ala Ile Ile Gln Lys Tyr Gly 
        515                 520                 525             


Ser Pro Gln Ala Val Asn Ile Glu Leu Ala Arg Glu Met Ser Arg Asn 
    530                 535                 540                 


Tyr Asp Glu Arg Arg Lys Met Glu Lys Gln Met Lys Asp Arg Gln Ser 
545                 550                 555                 560 


Glu Asn Glu Arg Ala Lys Lys Glu Ile Gln Glu Leu Gly Asn Ala Phe 
                565                 570                 575     


Pro Thr Gly Gln Asp Ile Leu Lys Tyr Arg Leu Trp His Asp Gln Asp 
            580                 585                 590         


Gly Ile Cys Met Tyr Ser Ala Lys Arg Ile Pro Leu Ala Glu Leu Phe 
        595                 600                 605             


Thr Gly Ala Tyr Asp Ile Asp His Ile Leu Pro Tyr Ser Ile Thr Phe 
    610                 615                 620                 


Asp Asp Ser Tyr Arg Asn Lys Val Leu Val Thr Ser Glu Glu Asn Arg 
625                 630                 635                 640 


Gln Lys Gly Asn Arg Ile Pro Tyr Glu Tyr Phe Gly Ser Asp Ser Glu 
                645                 650                 655     


Arg Trp Ser Lys Phe Glu Thr Leu Val Asn Ser Arg Ile Arg Asp Tyr 
            660                 665                 670         


Arg Lys Gln Gln Asn Leu Leu Lys Gln Ser Phe Thr Glu Glu Glu Arg 
        675                 680                 685             


Lys Glu Phe Lys Gln Arg Asn Leu Asn Asp Thr Lys Tyr Ile Thr Arg 
    690                 695                 700                 


Val Ile Tyr Asn Met Ile Arg Glu Asn Leu Ala Leu Ala Pro Tyr Asn 
705                 710                 715                 720 


Ser Pro Gly Lys Lys Lys Gln Val Phe Ala Val Asn Gly Gly Val Thr 
                725                 730                 735     


Ala Tyr Leu Arg Lys Arg Trp Gly Met Ser Ala Lys Asp Arg Ser Ile 
            740                 745                 750         


Asp Thr His His Ala Arg Asp Ala Val Val Ile Ala Cys Cys Thr Asp 
        755                 760                 765             


Gly Met Ile Gln Lys Ile Ser Arg Asn Ile Gln Gly Arg Glu Leu Leu 
    770                 775                 780                 


Tyr Ala Arg Gly Phe Thr Ala Val Asp Val Glu Thr Gly Glu Lys Phe 
785                 790                 795                 800 


Thr Arg Asp Asp Phe Thr Arg Asp Gln Trp Asp Glu Ile Phe Gly Val 
                805                 810                 815     


Gln Val Pro Lys Pro Trp Glu Tyr Phe Asn Glu Glu Leu Asp Ile Arg 
            820                 825                 830         


Leu Gly Glu Asn Pro Val Glu Phe Ile Glu Thr His Ser Asp Val Ser 
        835                 840                 845             


Arg Lys Leu Asp Tyr Pro Ala Trp Phe Tyr Glu Asn Asn Ile Ile Arg 
    850                 855                 860                 


Pro Val Phe Val Ser Arg Met Pro Asn Arg Lys Val Thr Gly Ala Ala 
865                 870                 875                 880 


His Ala Asp Thr Ile Arg Ser Pro Arg His Tyr Gln Glu Thr Gly Ser 
                885                 890                 895     


Val Leu Thr Lys Thr Ala Leu Ile Asp Leu Lys Leu Asn Lys Lys Thr 
            900                 905                 910         


Gly Glu Ile Glu Asn Tyr Tyr Lys Pro Glu Ser Asp Thr Leu Leu Tyr 
        915                 920                 925             


Asn Ala Leu Lys Glu Arg Leu Glu Leu Tyr Gly Gly Asp Gly Lys Lys 
    930                 935                 940                 


Ala Phe Glu Glu Pro Phe Tyr Lys Pro Lys Ala Asp Gly Ser Arg Gly 
945                 950                 955                 960 


Asn Leu Val Arg Lys Val Lys Thr Tyr Asp Lys Leu Thr Leu Gly Val 
                965                 970                 975     


Tyr Val Asn Ser Gln His Gly Ile Ala Ala Asn Ala Asn Gly Ser Met 
            980                 985                 990         


Val Arg Val Asp Val Phe Arg Val  Ser Gly Lys Tyr Tyr  Phe Val Pro 
        995                 1000                 1005             


Val Tyr  Ile Ala Asp Val Val  Arg Lys Arg Leu Pro  Met Lys Ala 
    1010                 1015                 1020             


Val Val  Gln Gly Lys Gln Tyr  Ile Asp Trp Lys Glu  Met Glu Asp 
    1025                 1030                 1035             


Lys Asp  Phe Ile Phe Ser Leu  Tyr Thr Arg Asp Leu  Ile Ser Phe 
    1040                 1045                 1050             


Lys Ser  Lys Asn Gly Lys Lys  Val Thr Cys Val Asp  Gly Thr Ser 
    1055                 1060                 1065             


Lys Ala  Val Asn Glu Glu Ile  Val Tyr Tyr Tyr Gly  Ala Asn Ile 
    1070                 1075                 1080             


Ser Thr  Ala Ser Phe Ser Gly  Lys Ser His Asn Gly  Ser Phe Glu 
    1085                 1090                 1095             


Phe Thr  Ser Leu Gly Ile Gln  Gly Leu Glu Tyr Leu  Lys Lys Tyr 
    1100                 1105                 1110             


Gln Val  Asp Val Leu Gly Asn  Val Ser Glu Val Lys  Arg Glu Lys 
    1115                 1120                 1125             


Arg Met  Gly Phe Arg 
    1130             


<210>  718
<211>  1133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  718

Met Gly Gly Phe Glu Val Asp Arg Lys Ile Ser Leu Gly Leu Asp Ile 
1               5                   10                  15      


Gly Val Ser Ser Val Gly Tyr Ser Val Ile Asp Val Asp Thr Gly Lys 
            20                  25                  30          


Val Leu Glu Leu Gly Ser Arg Leu Ile Asn Gly Lys Ile Gly Thr Glu 
        35                  40                  45              


Asn Gln Ala Arg Arg Glu Met Arg Gly Thr Arg Arg Leu Ile Asn Arg 
    50                  55                  60                  


Lys Lys Gln Arg Arg Lys Asp Thr Val Lys Leu Phe Asn Glu Phe Lys 
65                  70                  75                  80  


Leu Ile Asp Ser Lys Lys Asp Glu His Tyr Phe Asp His Phe Ala Asn 
                85                  90                  95      


Asn Ser Asp Pro Tyr Glu Leu Arg Val Lys Gly Leu Ser Glu Lys Leu 
            100                 105                 110         


Ser Leu Ser Glu Ile Ala Asp Gly Leu Tyr His Ile Val Lys Arg Arg 
        115                 120                 125             


Gly Ile Ser Tyr Asp Leu Lys Asp Ala Asp Glu Glu Ser Glu Gly Ser 
    130                 135                 140                 


Glu Tyr Glu Asn Gly Leu Lys Ile Asn Asn Ser Glu Leu Lys Asn Gln 
145                 150                 155                 160 


Thr Pro Ala Gln Ile Gln Leu Lys Arg Leu Asn Glu Tyr Gly Ala Ile 
                165                 170                 175     


Arg Gly Lys Val Val Ile Asp Glu Glu Lys Ile Leu Leu Asn Val Phe 
            180                 185                 190         


Pro Thr Lys Ala Tyr Leu Asp Glu Ala Lys Arg Ile Ile Glu Lys Gln 
        195                 200                 205             


Arg Glu Phe Tyr Pro Gly Ile Leu Thr Glu Glu Phe Glu Glu Lys Tyr 
    210                 215                 220                 


Cys Gln Ile Leu Thr Arg Lys Arg Asp Tyr Phe Val Gly Pro Gly Ser 
225                 230                 235                 240 


Glu Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Lys Glu Asp Gly Arg Thr 
                245                 250                 255     


Leu Asn Asn Leu Phe Glu Glu Leu Ile Gly His Asp Lys Ile Tyr Pro 
            260                 265                 270         


Glu Glu Leu Arg Ala Ser Gly Ala Ser Phe Thr Ala Gln Leu Phe Asn 
        275                 280                 285             


Val Leu Asn Asp Leu Asn Asn Leu His Ile Ser Ser Tyr Glu Asp Gln 
    290                 295                 300                 


Lys Leu Thr Thr Glu Asn Lys Glu Gln Ile Ile Ser Glu Leu Lys Ser 
305                 310                 315                 320 


Thr Thr Ser Asn Val Asn Met Met Lys Leu Ile Gln Lys Ile Ala Gly 
                325                 330                 335     


Cys Glu Glu Ser Asp Ile Ser Gly Tyr Arg Thr Asp Lys Asn Tyr Lys 
            340                 345                 350         


Pro Glu Ile Ser Ser Met Arg Ile Tyr Arg Lys Val His Lys Lys Leu 
        355                 360                 365             


Leu Glu Ser Glu Ile Asp Ile Thr Asn Trp Pro Asp Asp Phe Ile Asn 
    370                 375                 380                 


Lys Leu Ser Phe Ile Leu Thr Leu Asn Thr Glu Asn Gly Glu Ile Arg 
385                 390                 395                 400 


Asn Gln Leu Lys Asn Arg Leu Gln Pro Glu Tyr Asp Phe Leu Thr Asp 
                405                 410                 415     


Glu Leu Ile Gln Thr Ile Ile Asp Asn Lys Glu Ser Phe Asp Ile Lys 
            420                 425                 430         


Ser Asn Asn Lys Trp His Arg Phe Ser Ile Lys Thr Met Glu Leu Leu 
        435                 440                 445             


Ile Pro Glu Met Ile Lys Arg Pro Val Glu Gln Met Thr Leu Ile Asn 
    450                 455                 460                 


Glu Met Gly Leu Ile Lys Asn Ser Gly Lys Lys Phe Ser Asn Tyr Lys 
465                 470                 475                 480 


Tyr Leu Pro Tyr Lys Lys Ile Ala Glu Asp Ile Tyr Asn Pro Val Ala 
                485                 490                 495     


Ser Lys Ser Val Arg Glu Ala Leu Lys Ile Val Asn Ala Ile Leu Lys 
            500                 505                 510         


Glu Tyr Gly His Ile Glu Tyr Ile Val Val Glu Met Pro Arg Asp Lys 
        515                 520                 525             


Asn Glu Asp Glu Ala Lys Asp Gln Ile Glu Lys Phe Gln Lys Ala Asn 
    530                 535                 540                 


Gly Arg Leu Lys Asp Ala Ala Leu Glu Glu Phe Thr Glu Ala Ile Gly 
545                 550                 555                 560 


Asp Lys Ser Thr Val Asp Lys Lys Leu Gly Val His Arg Ser Lys Leu 
                565                 570                 575     


Leu Phe Lys Ile Arg Leu Trp Tyr Gln Gln Arg Gly Met Asp Leu Tyr 
            580                 585                 590         


Glu Gly Lys Pro Ile Ser Ala Val Asp Leu Leu Asn Asn Pro Asp Ser 
        595                 600                 605             


Phe Gln Ile Asp His Ile Ile Pro Gln Ser Val Ser Phe Asp Asp Ser 
    610                 615                 620                 


Phe Asn Asn Lys Thr Leu Cys Asn Ser Gly Leu Asn Gln Ile Lys Asp 
625                 630                 635                 640 


Lys Lys Thr Pro Phe Glu Tyr Met Asn Glu Gly Tyr Gly Gln Gly Phe 
                645                 650                 655     


Asp Ser Met Lys Ala Met Val Met Lys Asn Pro Arg Ile Asp His Asn 
            660                 665                 670         


Lys Arg Lys Asn Tyr Leu Phe Ser Glu Asn Val Ser Asp Ile Glu Thr 
        675                 680                 685             


Arg Lys Arg Phe Ile Ser Arg Asn Leu Val Asp Thr Arg Tyr Ala Ser 
    690                 695                 700                 


Arg Val Val Leu Asn Asn Leu Gln Glu Phe Phe Lys Glu Lys Glu Ser 
705                 710                 715                 720 


Asp Thr Lys Ile Ser Ile Ile Arg Gly Lys Phe Thr Ser Asn Leu Arg 
                725                 730                 735     


Lys His Trp Lys Ile Asn Lys Asn Arg Asp Thr Tyr His His His Ala 
            740                 745                 750         


Ile Asp Ala Thr Ile Ile Ala Ala Thr Pro Phe Leu Asn Ile Trp Lys 
        755                 760                 765             


Lys Gly Ser Ser Ile Phe Pro Ser Asn Val Glu Ser Glu Asp Phe Phe 
    770                 775                 780                 


Asp Ile Lys Thr Gly Lys Ile Ile Gly Asn Asp Glu Phe Asn Lys Tyr 
785                 790                 795                 800 


Ala Tyr Asp Glu Pro Tyr Asn Gly Phe Tyr Asn Glu Leu Arg Asn Ala 
                805                 810                 815     


Thr Asp Arg Ile Lys Phe Ser His Gln Ile Asp Lys Lys Met Asn Arg 
            820                 825                 830         


Lys Val Ser Asp Ser Thr Ile Tyr Ser Thr Arg Val Ala Lys Ser Pro 
        835                 840                 845             


Lys Asn Lys Lys Glu Ser Glu Tyr Ile Val Ala Lys Ile Lys Asn Ile 
    850                 855                 860                 


Tyr Asp Ala Asp Glu Tyr Ala Lys Phe Lys Arg Ile Tyr Asp Lys Asp 
865                 870                 875                 880 


Lys Thr Lys Phe Leu Leu Asn Lys Tyr Asp Pro Arg Ser Phe Asn His 
                885                 890                 895     


Leu Glu Lys Ile Met Asn Thr Tyr Pro Asp Ser Ile Glu Arg Thr Met 
            900                 905                 910         


Ser Asn Asn Lys Val Lys Val Val Arg Ile Ser Pro Phe Glu Leu Tyr 
        915                 920                 925             


Arg Gln Asp His Gly Pro Val Lys Lys Tyr Ser Lys Lys Asn Asn Gly 
    930                 935                 940                 


Pro Glu Ile Lys Gln Leu Lys Tyr Leu Asp Lys Lys Val Gly Ser Ser 
945                 950                 955                 960 


Ile Asp Val Thr Pro Asp Asn Ala Lys Asn Lys His Val Ile Leu Lys 
                965                 970                 975     


Ser Leu Lys Pro Trp Arg Thr Asp Ile Tyr Leu Asn His Glu Thr Gly 
            980                 985                 990         


Glu Tyr Glu Ile Met Gly Ile Lys  Tyr Ser Asp Leu Lys  Phe Asn Lys 
        995                 1000                 1005             


Gly Glu  Gly Tyr Gly Ile Lys  Lys Ala Lys Tyr Leu  Glu Ile Lys 
    1010                 1015                 1020             


His Arg  Glu Lys Val Ser Asp  Asp Ser Glu Phe Met  Phe Thr Leu 
    1025                 1030                 1035             


Tyr Lys  Lys Asn Arg Ile Lys  Val Glu Asn Met Glu  Thr Asp Glu 
    1040                 1045                 1050             


Ser Val  Glu Met Leu Phe Trp  Ser Arg Thr Met Asp  Ser Gln Lys 
    1055                 1060                 1065             


Gly Tyr  Ala Glu Leu Lys Pro  Ile Tyr Lys Tyr Lys  Thr Glu Asp 
    1070                 1075                 1080             


Asp Asn  Trp Pro Ile Tyr Gly  Asn Ala Lys Lys Gln  Ile Ile Lys 
    1085                 1090                 1095             


Lys Leu  Ile Pro Lys Asn Cys  Lys Ile Trp Lys Val  Asn Thr Asp 
    1100                 1105                 1110             


Val Leu  Gly Asn Pro Phe Tyr  Val Glu Lys Glu Gly  Glu Tyr Pro 
    1115                 1120                 1125             


Lys Asp  Val Leu Asp 
    1130             


<210>  719
<211>  1134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  719

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asn Ser Gly Lys Ile Val His Ala Ser Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Lys 
        35                  40                  45              


Asn Arg Gln Ala Arg Arg Leu His Arg Arg Lys Lys His Arg Gly Ala 
    50                  55                  60                  


Arg Leu Lys Asp Leu Phe Glu Tyr Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asp Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Thr Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Val Glu Asn Ser Glu 
                165                 170                 175     


Lys Cys Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Ala Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Asn Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Thr Ile 
    290                 295                 300                 


Ile Glu Tyr Ala Lys Ser Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Gly Glu Leu Ser 
        355                 360                 365             


Arg Asn Ile Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr Lys Leu Arg Asp Ser Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Val Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys His Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Tyr Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Asn Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Ser Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Glu Ser Lys Leu Leu Gly 
                645                 650                 655     


Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Asn His 
    690                 695                 700                 


Asn Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Pro Leu Ile Ala Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Gln Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Ser Ser Lys Thr Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Glu Lys Lys Glu Tyr Thr Tyr Thr Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Leu Gly Thr Lys Thr Pro Ser Lys Thr 
    850                 855                 860                 


Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Asn Lys Asp Lys Ser Gln Phe 
865                 870                 875                 880 


Leu Met Phe Gln Lys Asp Arg Lys Thr Trp Asp Glu Val Ile Glu Lys 
                885                 890                 895     


Ile Met Glu Gln Tyr Arg Pro Phe Lys Glu Tyr Asp Lys Ala Gly Lys 
            900                 905                 910         


Leu Val Asp Phe Asn Pro Phe Glu Lys Tyr Arg Gln Glu Asn Gly Pro 
        915                 920                 925             


Ile Arg Lys Tyr Ser Lys Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu 
    930                 935                 940                 


Lys Tyr Tyr Asp Ile Leu Leu Gly Lys His Lys Asn Ile Thr Pro Glu 
945                 950                 955                 960 


Gly Ser Arg Asn Thr Val Ala Leu Leu Ser Leu Asn Pro Trp Arg Thr 
                965                 970                 975     


Asp Val Tyr Tyr Asn Met Glu Thr Lys Lys Tyr Glu Phe Leu Gly Leu 
            980                 985                 990         


Lys Tyr Ala Asp Leu Pro Phe Glu  Glu Gly Gly Ala Tyr  Gly Ile Ser 
        995                 1000                 1005             


Thr Glu  Thr Tyr Asn Glu Leu  Arg Glu Lys Glu Gly  Ile Gly Lys 
    1010                 1015                 1020             


Asn Ser  Glu Phe Lys Phe Thr  Leu Tyr Lys Asn Asp  Leu Ile Leu 
    1025                 1030                 1035             


Ile Lys  Asp Thr Glu Thr Asn  Cys Gln Gln Phe Phe  Arg Phe Trp 
    1040                 1045                 1050             


Ser Arg  Thr Gly Lys Asp Asn  Pro Lys Ser Phe Glu  Lys His Lys 
    1055                 1060                 1065             


Ile Glu  Leu Lys Pro Tyr Glu  Lys Ala Lys Phe Glu  Lys Gly Glu 
    1070                 1075                 1080             


Glu Leu  Glu Val Leu Gly Lys  Val Pro Pro Ser Ser  Asn Gln Phe 
    1085                 1090                 1095             


Gln Lys  Asn Met Gln Ile Glu  Asn Leu Ser Ile Tyr  Lys Val Lys 
    1100                 1105                 1110             


Thr Asp  Val Leu Gly Asn Lys  His Phe Ile Lys Lys  Glu Gly Asp 
    1115                 1120                 1125             


Lys Pro  Lys Leu Lys Phe 
    1130                 


<210>  720
<211>  1134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  720

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asn Ser Gly Lys Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Lys 
        35                  40                  45              


Asn Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Lys His Arg Gly Val 
    50                  55                  60                  


Arg Phe Gln Asp Leu Phe Glu Asp Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asn Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Ala Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Leu Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Val Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Glu Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Gly Glu Leu Ser 
        355                 360                 365             


Arg Asn Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Met Glu Glu Ala Ile Asn Thr Lys Leu Lys Asp Ser Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys His Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Asn Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Thr Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Glu Ser Lys Leu Leu Gly 
                645                 650                 655     


Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Ile Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Ala His 
    690                 695                 700                 


Lys Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Ser Leu Ile Ser Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Glu Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Arg Ser Lys Lys Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Glu Lys Lys Glu Tyr Thr Tyr Thr Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Leu Gly Thr Lys Ala Pro Ser Lys Thr 
    850                 855                 860                 


Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Asn Lys Asp Lys Ser Gln Phe 
865                 870                 875                 880 


Leu Met Phe Gln Lys Asp Arg Lys Thr Trp Asp Glu Val Ile Glu Lys 
                885                 890                 895     


Ile Met Glu Gln Tyr Arg Pro Phe Lys Glu Tyr Asp Lys Thr Gly Lys 
            900                 905                 910         


Leu Val Asp Phe Asn Pro Phe Glu Lys Tyr Arg Gln Glu Asn Gly Pro 
        915                 920                 925             


Ile Arg Lys Tyr Ser Lys Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu 
    930                 935                 940                 


Lys Tyr Tyr Asp Ile Leu Leu Gly Lys His Lys Asn Ile Thr Pro Glu 
945                 950                 955                 960 


Gly Ser Arg Asn Thr Val Ala Leu Leu Ser Leu Asn Pro Trp Arg Thr 
                965                 970                 975     


Asp Val Tyr Tyr Asn Met Glu Thr Lys Lys Tyr Glu Phe Leu Gly Leu 
            980                 985                 990         


Lys Tyr Ala Asp Leu Pro Phe Glu  Glu Gly Gly Ala Tyr  Gly Ile Ser 
        995                 1000                 1005             


Thr Glu  Thr Tyr Asn Glu Leu  Arg Glu Lys Glu Gly  Ile Gly Lys 
    1010                 1015                 1020             


Asn Ser  Glu Phe Lys Phe Thr  Leu Tyr Lys Asn Asp  Leu Ile Leu 
    1025                 1030                 1035             


Ile Lys  Asp Thr Glu Thr Asn  Cys Gln Gln Ile Phe  Arg Phe Trp 
    1040                 1045                 1050             


Ser Arg  Thr Gly Lys Asp Asn  Pro Lys Ser Phe Glu  Lys His Lys 
    1055                 1060                 1065             


Ile Glu  Leu Lys Pro Tyr Glu  Lys Ala Arg Phe Glu  Lys Gly Glu 
    1070                 1075                 1080             


Glu Leu  Lys Val Leu Gly Lys  Val Pro Pro Ser Ser  Asn Gln Leu 
    1085                 1090                 1095             


Gln Lys  Asn Met Gln Ile Glu  Asn Leu Ser Ile Tyr  Lys Val Lys 
    1100                 1105                 1110             


Thr Asp  Val Leu Gly Asn Lys  His Phe Ile Lys Lys  Glu Gly Asp 
    1115                 1120                 1125             


Glu Pro  Lys Leu Lys Phe 
    1130                 


<210>  721
<211>  1134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  721

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asn Ser Gly Lys Ile Ile His Glu Asn Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Lys 
        35                  40                  45              


Asn Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Lys His Arg Gly Val 
    50                  55                  60                  


Arg Phe Gln Asp Leu Phe Glu Asp Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asn Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Thr Val Pro Ser Asp Tyr Gly Lys Ala Val Lys Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Glu Asn Gly Glu 
                165                 170                 175     


Lys Cys Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Gln Ile Thr 
        195                 200                 205             


Asn Glu Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr Arg Val Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Ile Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Asp Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Glu Thr Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Gly Glu Leu Ser 
        355                 360                 365             


Arg Asn Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr Lys Leu Met Gly Val Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Val Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Ser Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Asp Ser Lys Leu Leu Ser 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Ala His 
    690                 695                 700                 


Lys Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Pro Leu Ile Thr Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Glu Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Arg Ser Lys Thr Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Glu Lys Lys Glu Tyr Thr Tyr Thr Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Leu Gly Thr Lys Thr Pro Ser Lys Thr 
    850                 855                 860                 


Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Asn Lys Asp Lys Ser Gln Phe 
865                 870                 875                 880 


Leu Met Phe Gln Lys Asp Arg Lys Thr Trp Asp Glu Val Ile Glu Lys 
                885                 890                 895     


Ile Met Glu Gln Tyr Arg Pro Phe Lys Glu Tyr Asp Lys Thr Gly Lys 
            900                 905                 910         


Leu Val Asp Phe Asn Pro Phe Glu Lys Tyr Arg Gln Glu Asn Gly Pro 
        915                 920                 925             


Ile Cys Lys Tyr Ser Lys Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu 
    930                 935                 940                 


Lys Tyr Tyr Asp Ile Leu Leu Gly Lys His Lys Asn Ile Thr Pro Glu 
945                 950                 955                 960 


Gly Ser Arg Asn Thr Val Ala Leu Leu Ser Leu Asn Pro Trp Arg Thr 
                965                 970                 975     


Asp Val Tyr Tyr Asn Met Glu Thr Lys Lys Tyr Glu Phe Leu Gly Leu 
            980                 985                 990         


Lys Tyr Ala Asp Leu Pro Phe Glu  Lys Gly Gly Ala Tyr  Gly Ile Ser 
        995                 1000                 1005             


Thr Glu  Thr Tyr Asn Lys Leu  Arg Glu Lys Glu Gly  Ile Gly Lys 
    1010                 1015                 1020             


Asn Ser  Glu Phe Lys Phe Thr  Leu Tyr Lys Asn Asp  Leu Ile Leu 
    1025                 1030                 1035             


Ile Lys  Asp Thr Glu Thr Asn  Cys Gln Gln Ile Phe  Arg Phe Trp 
    1040                 1045                 1050             


Ser Arg  Thr Gly Lys Asp Asn  Pro Lys Ser Phe Glu  Lys His Lys 
    1055                 1060                 1065             


Ile Glu  Leu Lys Pro Tyr Glu  Lys Ala Arg Phe Glu  Lys Gly Glu 
    1070                 1075                 1080             


Glu Leu  Glu Val Leu Gly Lys  Val Pro Pro Ser Ser  Asn Gln Phe 
    1085                 1090                 1095             


Gln Lys  Asn Met Gln Ile Glu  Asn Leu Ser Ile Tyr  Lys Val Lys 
    1100                 1105                 1110             


Thr Asp  Val Leu Gly Lys Lys  His Ile Ile Lys Lys  Glu Gly Asp 
    1115                 1120                 1125             


Glu Pro  Lys Leu Lys Phe 
    1130                 


<210>  722
<211>  1134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  722

Met Lys Tyr Ile Glu Ser Val Ser Ile Gly Phe Asp Ile Gly Val Gly 
1               5                   10                  15      


Ser Val Gly Tyr Ala Val Ile Asn Ser Lys Thr Lys Gln Ile Leu Glu 
            20                  25                  30          


Ser Gly Val Ser Ile Phe Pro Ala Ala Val Ala Gln Lys Asn Val Glu 
        35                  40                  45              


Arg Arg Glu Ser Arg Gln Ser Arg Arg Leu Arg Arg Arg Arg Val Asn 
    50                  55                  60                  


Arg Leu Asn Asp Leu Lys Lys Leu Leu Lys Lys Asn Gly Phe Ile Pro 
65                  70                  75                  80  


Ser Pro Asn Lys Asn Pro Tyr Glu Ile Arg Val Arg Gly Leu Glu His 
                85                  90                  95      


Gln Leu Ser Arg Asp Glu Ile Ala Ile Ala Val Leu His Ile Ala Lys 
            100                 105                 110         


Arg Arg Gly Ile Ser Tyr Ser Leu Asp Asp Val Val Asp Glu Asn Val 
        115                 120                 125             


Thr Asp Phe Lys Gly Lys Ile Ala Lys Asn Gln Glu Ala Leu Lys Asn 
    130                 135                 140                 


Lys Thr Pro Gly Glu Ile Gln Tyr Glu Arg Leu Leu Lys Tyr Gly Lys 
145                 150                 155                 160 


Val Arg Gly Thr Val Thr Asn Tyr Val Glu Tyr Asp Glu Gln Pro Glu 
                165                 170                 175     


Tyr Phe Leu Asn Val Phe Pro Asn Ser Ala Tyr Val Asp Glu Ala Lys 
            180                 185                 190         


Arg Ile Leu Thr Cys Gln Lys Gln Tyr Tyr Pro Glu Ile Thr Asp Asp 
        195                 200                 205             


Leu Ile Glu Asn Ile Val Lys Ile Ile Ser Arg Lys Arg Ala Tyr Tyr 
    210                 215                 220                 


Ile Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Arg 
225                 230                 235                 240 


Thr Asn Gly Glu Thr Leu Glu Asn Ile Phe Glu Ile Leu Ile Gly Lys 
                245                 250                 255     


Asp Ser Val Asn Pro Asp Glu Leu Arg Ala Pro Ala Asn Ser Leu Thr 
            260                 265                 270         


Ala Gln Val Tyr Asn Leu Leu Asn Asp Leu Thr Asn Ile Arg Tyr Asn 
        275                 280                 285             


Gly Ser Glu Arg Leu Thr Pro Glu Gln Lys Gln Asp Ile Leu Ser Glu 
    290                 295                 300                 


Leu Leu Thr Thr Glu Lys Thr Val Gln Met Leu Asn Val Ile Ala Lys 
305                 310                 315                 320 


Lys Val Asn Val Lys Lys Glu Glu Ile Ser Gly Phe Arg Thr Lys Lys 
                325                 330                 335     


Asp Asp Lys Pro Asp Phe His Thr Leu Glu Val Tyr Arg Lys Val Arg 
            340                 345                 350         


Lys His Met Leu Asn Lys Gly Ile Asp Ile Asn Glu Trp Pro Thr Glu 
        355                 360                 365             


Cys Phe Asp Glu Leu Ala Phe Ile Phe Asn Leu Asn Thr Glu Ile Gly 
    370                 375                 380                 


Glu Ile Arg Asn Arg Ile Ser Glu Lys Val Val Pro Lys Tyr Asp Phe 
385                 390                 395                 400 


Ile Thr Asn Gln Ile Val Asp Asp Leu Leu Glu Asp Pro Lys Ile Phe 
                405                 410                 415     


Ala Ile Ser Thr Ser Gln Lys Trp His Arg Phe Ser Ile Lys Thr Met 
            420                 425                 430         


Asn Val Leu Ile Pro Glu Leu Leu Asn Gly Tyr Asn Glu Gln Met Thr 
        435                 440                 445             


Ile Leu Thr Arg Met Gly Leu Leu Glu Asp Ser Lys Asn Asp Tyr Ser 
    450                 455                 460                 


Asn Ile Asp Lys Ile Asp Ile Lys Asn Val Thr Glu Asn Ile Tyr Asn 
465                 470                 475                 480 


Pro Val Val Arg Lys Ser Ile Lys Lys Cys Leu Lys Ile Phe Asn Glu 
                485                 490                 495     


Leu Tyr Lys Lys Tyr Pro Asn Ile Ser Asp Val Val Ile Glu Leu Pro 
            500                 505                 510         


Arg Glu Asp Asn Pro Glu Asp Ala Arg Lys Leu Ile Lys Lys Met Gln 
        515                 520                 525             


Lys Glu Asn Leu Val Glu Lys Asp Thr Ser Leu Glu Ala Phe Arg Lys 
    530                 535                 540                 


Glu Ala Asn Leu Ser Glu Asn Gly Leu Ile Asp Phe Leu Arg Lys Gly 
545                 550                 555                 560 


Lys Asn Arg Ala Leu Lys Ile Arg Leu Trp Tyr Gln Gln Glu Gly Lys 
                565                 570                 575     


Cys Leu Tyr Thr Gly Glu Pro Ile Leu Ala Glu Asp Leu Cys Lys Asn 
            580                 585                 590         


Glu Ala Asn Tyr Asp Ile Asp His Ile Ile Pro Ile Ser Val Ser Phe 
        595                 600                 605             


Asp Asp Ser Ile Asn Asn Lys Val Leu Cys Ser Ser His Phe Asn Arg 
    610                 615                 620                 


Phe Glu Lys Glu Lys Lys Thr Pro Phe Glu Tyr Phe Ser Asn Lys Ser 
625                 630                 635                 640 


Ser His Leu Gly Arg Tyr Ser Asn Phe Asp Glu Phe Arg Ala Ser Val 
                645                 650                 655     


Asn Ala Asn Lys Arg Ile Ser Asp Val Lys Arg Lys Asn Leu Leu Ser 
            660                 665                 670         


Lys Glu Leu Leu Ser Asp Leu Asp Thr Arg Lys Lys Phe Ile Ala Arg 
        675                 680                 685             


Asn Leu Val Asp Thr Arg Tyr Ala Ser Arg Val Val Phe Asn Glu Leu 
    690                 695                 700                 


Thr Gln Phe Phe Ala Ser Lys Lys Ala Asp Thr Lys Val Ala Val Ile 
705                 710                 715                 720 


Arg Gly Lys Phe Thr Ser Gln Leu Arg Arg Ala Trp Gln Leu Asn Lys 
                725                 730                 735     


Ser Arg Asp Thr Tyr His His His Ala Val Asp Ala Ala Leu Ile Gly 
            740                 745                 750         


Val Thr Pro Phe Leu Lys Ile Trp Arg Ser Gly Lys Thr Ile Tyr Pro 
        755                 760                 765             


Arg Lys Met Thr Glu Ala Gln Thr Asp Phe Glu Leu Asn Glu Ile Thr 
    770                 775                 780                 


Thr Asn Asp Glu Phe Lys Glu Ser Tyr Tyr Gln Leu Pro Cys Pro Gln 
785                 790                 795                 800 


Phe Leu Asp Tyr Leu Thr Gln Met Gln Glu Asn Leu Lys Ile Asn Phe 
                805                 810                 815     


Glu Val Asp Lys Lys Met Asn Arg Lys Ile Ser Asp Ala Met Ile Tyr 
            820                 825                 830         


Ser Ser Arg Lys Lys Lys Leu Ser Lys Asp Lys Lys Glu Lys Glu Tyr 
        835                 840                 845             


Ser Leu Gly Thr Ile Asn Asp Ile Tyr Ser Asp Ala Gly Tyr Ser Lys 
    850                 855                 860                 


Phe Lys Asn Ile Tyr Lys Lys Asp Lys Glu Lys Phe Leu Met Tyr His 
865                 870                 875                 880 


Leu Asp His Lys Ser Phe Glu Ile Leu Glu Asp Ile Ile Ser Gln Tyr 
                885                 890                 895     


Pro Asp Lys Glu Glu Tyr Ile Asp Ser Glu Gly Lys Val Lys Gln Arg 
            900                 905                 910         


Ala Val Ser Pro Phe Glu Ile Tyr Arg Arg Lys Tyr Gly Tyr Ile Arg 
        915                 920                 925             


Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Ile Lys Asn Met Lys Tyr 
    930                 935                 940                 


Tyr Asn Glu Asn Leu Pro Lys Ile Tyr Ile Asp Ile Thr Pro Lys Gln 
945                 950                 955                 960 


Ser Ile Asp Lys Lys Val Tyr Leu Thr Asn Ile Ser Pro Trp Arg Thr 
                965                 970                 975     


Asp Val Tyr Tyr Asn Arg Ser Gln Asn Ser Tyr His Ile Met Gly Ile 
            980                 985                 990         


Lys Tyr Ser Asp Leu Arg Tyr Ile  Asp Gly Lys Tyr Gly  Ile Pro Lys 
        995                 1000                 1005             


Asn Val  Tyr Glu Lys Ile Lys  Leu Arg Glu Lys Ile  Pro Ser Glu 
    1010                 1015                 1020             


Ser Glu  Phe Cys Phe Ser Leu  Tyr Lys Asn Asp Arg  Val Lys Ile 
    1025                 1030                 1035             


Ile Asp  Pro Asn Lys Asn Glu  Glu Ile Glu Leu Arg  Phe Asn Ser 
    1040                 1045                 1050             


Arg Thr  Leu Pro Ser Val Leu  Asn Tyr Val Glu Phe  Lys Pro Leu 
    1055                 1060                 1065             


Phe Gln  Glu Ser Tyr Asn Ser  Leu Thr Leu Leu Pro  Val Phe Ser 
    1070                 1075                 1080             


Lys Thr  Thr Ser Glu Gly Arg  Ile Ile Lys Lys Phe  Thr Arg Glu 
    1085                 1090                 1095             


Asp Asn  Ile Val Tyr Lys Val  Asn Thr Asp Glu Leu  Gly Asn Pro 
    1100                 1105                 1110             


Tyr Tyr  Ile Leu Lys Glu Ser  Gln Asn Pro Lys Asn  Ile Ile Asp 
    1115                 1120                 1125             


Asn Ser  Asn Lys Thr Glu 
    1130                 


<210>  723
<211>  1134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  723

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Glu Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asn Ser Gly Lys Ile Ile His Ala Asn Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Lys 
        35                  40                  45              


Asn Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Lys His Arg Gly Val 
    50                  55                  60                  


Arg Phe Gln Asp Leu Phe Glu Asp Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asn Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Ala Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Leu Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Val Ile 
    290                 295                 300                 


Val Glu Tyr Ala Lys Glu Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Ser Val Gly Glu Leu Ser 
        355                 360                 365             


Arg Asn Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Met Glu Glu Ala Ile Asn Thr Lys Leu Lys Asp Ser Phe 
385                 390                 395                 400 


Ser Gln Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys His Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Asn Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Thr Ile Pro Ile Ser Asp Leu Ile Tyr Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Ala Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Asp Leu Lys Ser Leu Ser 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Ser His 
    690                 695                 700                 


Gln Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys Gln Asn Asn Pro Leu Ile Thr Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Gln Thr Gly Glu Ile Ile Ser Leu Thr Asp Asp Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Ser Ser Lys Thr Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Glu Lys Lys Glu Tyr Thr Tyr Thr Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ser Leu Gly Thr Lys Thr Pro Ser Lys Thr 
    850                 855                 860                 


Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Asn Lys Asp Lys Ser Gln Phe 
865                 870                 875                 880 


Leu Met Phe Gln Lys Asp Arg Lys Thr Trp Asp Glu Val Ile Glu Lys 
                885                 890                 895     


Ile Met Glu Gln Tyr Arg Pro Phe Lys Glu Tyr Asp Lys Thr Gly Lys 
            900                 905                 910         


Leu Val Asp Phe Asn Pro Phe Glu Lys Tyr Arg Gln Glu Asn Glu Pro 
        915                 920                 925             


Ile Arg Lys Tyr Ser Lys Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu 
    930                 935                 940                 


Lys Tyr Tyr Asp Ile Leu Leu Gly Lys His Lys Asn Ile Thr Pro Glu 
945                 950                 955                 960 


Gly Ser Arg Asn Thr Val Ala Leu Leu Ser Leu Asn Pro Trp Arg Thr 
                965                 970                 975     


Asp Val Tyr Tyr Asn Met Glu Thr Lys Lys Tyr Glu Phe Leu Gly Leu 
            980                 985                 990         


Lys Tyr Ala Asp Leu Pro Phe Glu  Glu Gly Gly Ala Tyr  Gly Ile Ser 
        995                 1000                 1005             


Thr Glu  Thr Tyr Asn Glu Leu  Arg Glu Lys Glu Gly  Ile Gly Lys 
    1010                 1015                 1020             


Asn Ser  Glu Phe Lys Phe Thr  Leu Tyr Lys Asn Asp  Leu Ile Leu 
    1025                 1030                 1035             


Ile Lys  Asp Thr Glu Thr Asn  Cys Gln Gln Ile Phe  Arg Phe Trp 
    1040                 1045                 1050             


Ser Arg  Thr Gly Lys Asp Asn  Pro Lys Ser Phe Glu  Lys His Lys 
    1055                 1060                 1065             


Ile Glu  Leu Lys Pro Tyr Glu  Lys Ala Arg Phe Glu  Lys Gly Glu 
    1070                 1075                 1080             


Glu Leu  Glu Val Leu Gly Lys  Val Pro Pro Ser Ser  Asn Gln Leu 
    1085                 1090                 1095             


Gln Lys  Asn Met Gln Ile Glu  Asn Leu Ser Ile Tyr  Lys Val Lys 
    1100                 1105                 1110             


Thr Asp  Val Leu Gly Asn Lys  His Phe Ile Lys Lys  Glu Gly Asp 
    1115                 1120                 1125             


Lys Pro  Lys Leu Lys Phe 
    1130                 


<210>  724
<211>  1135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  724

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Glu Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn His Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Ala Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Glu Asp Gly Arg 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr His Ala Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Ser Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Lys 
225                 230                 235                 240 


Tyr Thr Thr Lys Lys Asp Ala Gln Gly Gln Tyr Ile Thr Leu Asn Asn 
                245                 250                 255     


Ile Phe Gly Ile Leu Ile Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr 
            260                 265                 270         


Arg Ala Ala Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Glu 
    290                 295                 300                 


Glu Gln Lys Tyr Gln Ile Ile Thr Tyr Val Lys Asn Glu Lys Ala Met 
305                 310                 315                 320 


Gly Pro Ala Lys Leu Phe Lys Tyr Ile Ala Lys Leu Leu Ala Cys Asp 
                325                 330                 335     


Ile Ala Asp Ile Lys Gly Tyr Arg Ile Asp Lys Ser Asp Lys Ala Glu 
            340                 345                 350         


Ile His Thr Phe Glu Ala Tyr Arg Lys Met Lys Thr Leu Glu Thr Ile 
        355                 360                 365             


Asp Ile Glu Gln Ile Glu Arg Glu Lys Leu Asp Lys Leu Ala Tyr Val 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Arg Glu Gly Ile Gln Glu Ala Leu Asp His 
385                 390                 395                 400 


Glu Phe Ala Asp Gly Thr Phe Ser Gln Glu Gln Val Asp Glu Leu Val 
                405                 410                 415     


Gln Phe Arg Lys Ala Asn Ser Ser Ile Phe Gly Lys Gly Trp His Ser 
            420                 425                 430         


Phe Ser Val Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Ala Thr 
        435                 440                 445             


Ser Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr 
    450                 455                 460                 


Thr Ser Ser Ser Asn Lys Thr Lys Tyr Ile Asp Glu Lys Gln Leu Thr 
465                 470                 475                 480 


Glu Glu Ile Tyr Asn Pro Val Val Ala Lys Ser Val Arg Gln Ala Ile 
                485                 490                 495     


Lys Ile Val Asn Ala Ala Ile Lys Lys Tyr Gly Asp Phe Asp Asn Ile 
            500                 505                 510         


Val Ile Glu Met Ala Arg Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala 
        515                 520                 525             


Ile Gln Lys Ile Gln Lys Ala Asn Lys Ala Glu Lys Asp Ala Ala Met 
    530                 535                 540                 


Arg Lys Ala Ala Asn Gln Tyr Asn Gly Lys Ala Glu Leu Pro His Ser 
545                 550                 555                 560 


Val Phe His Gly His Lys Gln Leu Ala Thr Lys Ile Arg Leu Trp His 
                565                 570                 575     


Gln Gln Gly Glu Arg Cys Leu Tyr Thr Gly Lys Thr Ile Ser Ile His 
            580                 585                 590         


Asp Leu Ile Asn Asn Pro Asn Gln Phe Glu Ile Asp His Ile Leu Pro 
        595                 600                 605             


Leu Ser Ile Thr Phe Asp Asp Ser Leu Ala Asn Lys Val Leu Val Tyr 
    610                 615                 620                 


Ala Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu 
625                 630                 635                 640 


Asp Ser Met Asp Asp Ala Trp Ser Phe Arg Glu Leu Lys Ala Phe Val 
                645                 650                 655     


Arg Glu Ser Lys Ala Leu Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr 
            660                 665                 670         


Glu Glu Asp Ile Ser Lys Phe Asp Val Arg Lys Lys Phe Ile Glu Arg 
        675                 680                 685             


Asn Leu Val Asp Thr Arg Tyr Ala Ser Arg Val Val Leu Asn Ala Leu 
    690                 695                 700                 


Gln Glu His Phe Lys Ala His Lys Ile Asp Thr Lys Val Ser Val Val 
705                 710                 715                 720 


Arg Gly Gln Phe Thr Ser Gln Leu Arg Arg His Trp Gly Ile Asp Lys 
                725                 730                 735     


Thr Arg Asp Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala 
            740                 745                 750         


Ala Ser Ser Gln Leu Asn Leu Trp Lys Lys Gln Lys Asn Thr Leu Val 
        755                 760                 765             


Ser Tyr Ser Glu Asp Gln Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile 
    770                 775                 780                 


Ser Asp Asp Glu Tyr Lys Glu Ser Val Phe Lys Ala Pro Tyr Gln His 
785                 790                 795                 800 


Phe Val Asp Thr Leu Lys Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe 
                805                 810                 815     


Ser Tyr Gln Val Asp Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr 
            820                 825                 830         


Ile Tyr Ala Thr Arg Gln Ala Lys Val Gly Lys Asp Lys Lys Asp Glu 
        835                 840                 845             


Thr Tyr Val Leu Gly Lys Ile Lys Asp Ile Tyr Ala Leu Gly Thr Lys 
    850                 855                 860                 


Thr Pro Ser Lys Thr Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Asn Lys 
865                 870                 875                 880 


Asp Lys Ser Gln Phe Leu Val Tyr Gln Lys Asp Arg Lys Thr Trp Asp 
                885                 890                 895     


Glu Val Ile Glu Lys Ile Met Glu Gln Tyr Arg Pro Phe Lys Glu Ser 
            900                 905                 910         


Asp Lys Asn Gly Lys Glu Val Asp Phe Asn Pro Phe Glu Lys Tyr Arg 
        915                 920                 925             


Ile Glu Asn Gly Pro Ile Arg Lys Tyr Ser Arg Lys Gly Asn Gly Pro 
    930                 935                 940                 


Glu Ile Lys Ser Leu Lys Tyr Tyr Ala Ser Lys Leu Gly Asn His Ile 
945                 950                 955                 960 


Asp Ile Thr Pro Lys Asp Ser Asn Asn Lys Val Val Leu Gln Ser Val 
                965                 970                 975     


Ser Pro Trp Arg Ala Asp Val Tyr Phe Asn Lys Thr Thr Gly Ser Tyr 
            980                 985                 990         


Glu Ile Leu Gly Leu Lys Tyr Ala  Asp Leu Gln Phe Glu  Lys Gly Thr 
        995                 1000                 1005             


Gly Thr  Tyr Arg Ile Ser Glu  Glu Lys Tyr Asn Asp  Ile Lys Lys 
    1010                 1015                 1020             


Lys Glu  Gly Val Asp Ser Asp  Ser Glu Phe Lys Phe  Thr Leu Tyr 
    1025                 1030                 1035             


Lys Asn  Asp Leu Leu Leu Ile  Lys Asp Thr Glu Thr  Lys Glu Gln 
    1040                 1045                 1050             


Arg Leu  Phe Arg Phe Leu Ser  Arg Ser Met Pro Asn  Lys Lys His 
    1055                 1060                 1065             


Tyr Val  Glu Leu Lys Pro Tyr  Ser Lys Asp Lys Phe  Glu Lys Asn 
    1070                 1075                 1080             


Glu Ser  Leu Ile Glu Ile Leu  Gly Ser Ala Asp Lys  Ser Gly Arg 
    1085                 1090                 1095             


Cys Ile  Lys Gly Leu Ala Lys  Ser Asn Leu Ser Ile  Phe Lys Val 
    1100                 1105                 1110             


Arg Thr  Asp Val Leu Gly Asn  Gln His Ile Ile Lys  Asn Glu Gly 
    1115                 1120                 1125             


Asp Lys  Pro Lys Leu Asp Phe  
    1130                 1135 


<210>  725
<211>  1135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  725

Met Gly Val Ile Phe Leu Tyr Glu Asn Ile Val Lys Pro Phe Pro Ser 
1               5                   10                  15      


Arg Tyr Asp Lys Ala Ile Met Lys Arg Leu Lys Gly Arg Gln Thr Met 
            20                  25                  30          


Gly Tyr Ile Leu Ser Leu Asp Ile Gly Ile Ala Ser Cys Gly Tyr Ser 
        35                  40                  45              


Val Leu Glu Thr Asp Ala Leu Gly Val Pro Tyr Lys Ile Lys Thr Leu 
    50                  55                  60                  


Asn Ser Ile Ile Phe Thr Arg Ala Glu Asn Pro Lys Thr Gly Ala Ser 
65                  70                  75                  80  


Leu Ala Glu Pro Arg Arg Lys Tyr Arg Gly Ala Arg Arg Arg Lys Arg 
                85                  90                  95      


Arg Thr Ser Phe Arg Lys His Arg Thr Arg Gln Leu Phe Ile Thr Ser 
            100                 105                 110         


Gly Leu Leu Ser Lys Gln Asp Ile Ala Arg Tyr Leu Asn Gln Gln Glu 
        115                 120                 125             


Pro His Asp Asp Ile Trp Glu Leu Arg Val Lys Gly Leu Asp Glu Pro 
    130                 135                 140                 


Leu Thr Asn Leu Gln Leu Phe Gln Val Leu Tyr Tyr Phe Val Thr His 
145                 150                 155                 160 


Arg Gly Phe Lys Ser Asn Arg Lys Ala Glu Leu Lys Asp Lys Asp Ile 
                165                 170                 175     


Gly Pro Val Leu Asn Ser Ile Asn Ala Phe Lys Glu Glu Met Gln Ser 
            180                 185                 190         


His Asp Tyr Arg Thr Ile Gly Glu Leu Tyr Ala Asn Asp Pro Arg Phe 
        195                 200                 205             


Arg Asp Arg Lys His Asn Lys Asp Tyr Thr Asn Gly Phe Leu Gly Thr 
    210                 215                 220                 


Ile Gln Arg Ser Trp Leu Val Asp Glu Ile Lys Met Leu Leu Thr Asn 
225                 230                 235                 240 


Gln Gln Ser Phe Gly Asn Thr Gln Leu Thr Ser Glu Phe Ile Glu Leu 
                245                 250                 255     


Tyr Leu Asn Ile Phe Thr Ser Gln Arg Asn Phe Asp Asp Gly Pro Ala 
            260                 265                 270         


Ser Gly Pro Tyr Ser Gly Asp Leu Ile Asp Lys Met Val Gly Pro Asp 
        275                 280                 285             


Thr Phe Asn Arg Ser Arg Lys Arg Ala Pro Arg Ala Thr Tyr Thr Phe 
    290                 295                 300                 


Gln Tyr Phe Ser Phe Leu Ser Lys Leu Asn Ser Leu Arg Ile Ala Pro 
305                 310                 315                 320 


Ser Ile Ser Ala Pro Phe Glu Thr Leu Thr Val Asp Gln Arg Ala Leu 
                325                 330                 335     


Ile Thr Lys Arg Leu Trp Gly Pro Asp Val Lys Ser Ala Asp Leu Lys 
            340                 345                 350         


Phe Lys Gln Ile Arg Met Val Leu Ser Leu Pro Glu Thr Ala Arg Phe 
        355                 360                 365             


Asn Leu Val Asn Tyr Gly Asn Thr Pro Glu Lys Ala Leu Glu Ile Glu 
    370                 375                 380                 


Asp Lys Thr Thr Phe Ile Ser Ile Lys Pro Leu Arg Glu Ile Glu Lys 
385                 390                 395                 400 


Ala Leu Thr Gly Thr Thr Leu Pro Val Ser His Asp Gln Leu Asp Ala 
                405                 410                 415     


Ile Ala Arg Ile Leu Thr Val Tyr Lys Ser Asp Ser Asn Arg Lys Thr 
            420                 425                 430         


Ala Phe His Asn Gln Thr Val Leu Ser Asp Glu Val Ile Glu Gln Leu 
        435                 440                 445             


Leu Pro Leu Asn Phe Ser Lys Phe Gly His Leu Ser Val Glu Thr Met 
    450                 455                 460                 


Gln Glu Ile Ile Pro Tyr Leu Glu Gln Gly Met Val Tyr Ser Asp Ala 
465                 470                 475                 480 


Ala Thr Ala Ala Gly Tyr Asp Phe Arg Asn Asn Gln Ile Asp Met Glu 
                485                 490                 495     


Tyr Ile Arg Asp Asn Val Thr Asn Pro Val Val Arg Arg Ala Val Ser 
            500                 505                 510         


Val Ala Asn Lys Leu Val Lys Ala Val Val Arg Arg Tyr Gly Lys Pro 
        515                 520                 525             


Asp Ser Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Asn Phe Gln 
    530                 535                 540                 


Glu Arg Arg Gln Asp Glu Val Arg Gln Gln Lys Asn Ala Arg Asp Asn 
545                 550                 555                 560 


Glu Lys Ile Ala Lys Arg Leu Glu Glu Leu Gly Ile Pro Val Asn Gly 
                565                 570                 575     


Val Asn Ile Thr Lys Met Lys Leu Tyr Asp Glu Gln His Gly Ile Asp 
            580                 585                 590         


Leu Tyr Asp Leu Ser Thr Asn Ala Ser Met Ala Val Thr Asp Ile Phe 
        595                 600                 605             


Ser Asp Asp Arg Tyr Glu Ile Asp His Ile Ile Pro Tyr Ser Val Ser 
    610                 615                 620                 


Phe Asp Asp Ser Tyr Thr Asn Lys Val Leu Val Ser Ala Lys Ala Asn 
625                 630                 635                 640 


Arg Glu Lys Arg Asp Arg Ile Pro Met Glu Val Phe Gly Asn Asp Ser 
                645                 650                 655     


Glu Arg Arg Arg Gln Phe Glu Asn Arg Val Asn Thr Asn Ile Thr Asn 
            660                 665                 670         


Leu Arg Lys Lys Arg Asn Leu Leu Lys Glu Thr Phe Ser Asp Glu Asp 
        675                 680                 685             


Lys Ala Lys Trp Lys Ser Arg Asn Ile Asn Asp Thr Arg Tyr Leu Asn 
    690                 695                 700                 


Arg Ile Leu Met Asn Tyr Leu Ser Gln Asn Val Glu Phe Thr Asp Val 
705                 710                 715                 720 


Leu Ser Asp Ala Lys His Val Ile Ala Leu Asn Gly Ala Val Thr Ala 
                725                 730                 735     


Lys Ile Arg Ser Arg Trp Gly Ile Thr Lys Val Arg Ala Ala Gly Asp 
            740                 745                 750         


Leu His His Ala Val Asp Ala Val Val Ile Gly Cys Val Thr Asp Ala 
        755                 760                 765             


Leu Ile Gln Ala Val Thr Arg Tyr Ala Lys Tyr Gln Glu Arg Arg Asp 
    770                 775                 780                 


Asn Lys Ala Ala Ala Glu Gln Asp Leu Ala Glu Asn Asn Pro Glu Glu 
785                 790                 795                 800 


Leu Asp Ala Lys Gln Phe Arg Lys Val Phe Asn Asp Tyr Pro Leu Pro 
                805                 810                 815     


Trp Pro Glu Phe Arg Glu Glu Leu Met Gly Arg Val Ser Ser Asp Pro 
            820                 825                 830         


Ser Gln Ile Met Ala Gly Arg Gln Trp Ser His Tyr Thr Ala Glu Glu 
        835                 840                 845             


Ile Ser Glu Leu Lys Gln Pro Phe Val Val Arg Val Pro Asn His Arg 
    850                 855                 860                 


Ile Val Gly Pro Ala Thr Leu Asp Thr Val Tyr Gly Gln Pro Asn Pro 
865                 870                 875                 880 


Asp Asp Gly Leu Ile Thr Gln Arg Val Arg Ile Thr Asp Leu Lys Pro 
                885                 890                 895     


Gln Lys Asn Lys Asp Gly Glu Asp Ile Ile Lys Ala Gly Ala Asn Val 
            900                 905                 910         


Tyr Glu Leu Ala Ala Asp Gly Gly Asn Lys Leu Val Tyr Glu Ser Ile 
        915                 920                 925             


Leu Asp Ala Leu Gln Val Ala Gly Gly Asp Gly Lys Lys Ala Phe Pro 
    930                 935                 940                 


Thr Gly Arg Leu Leu Val Arg Ser Pro Ile Gln Asp Ile Pro Val Glu 
945                 950                 955                 960 


Arg Val Arg Val Thr Ser Lys Ala Thr Leu Val Val Pro Val Gly Pro 
                965                 970                 975     


Lys Ser Val Ala Ala Asn Gly Ser Met Val Arg Ile Asp Val Tyr Lys 
            980                 985                 990         


Thr Pro Lys Lys Tyr Val Phe Val  Pro Ile Tyr Val Arg  Asp Thr Val 
        995                 1000                 1005             


Lys Ala  Glu Leu Pro Asp Arg  Ala Val Ala Pro Val  Lys Pro Tyr 
    1010                 1015                 1020             


Ser Gln  Trp Tyr Gln Leu Gln  Pro Lys Asp Gln Phe  Leu Phe Ser 
    1025                 1030                 1035             


Leu Phe  Ala Asn Asp Leu Ile  Arg Phe Lys His Pro  Arg Gly Val 
    1040                 1045                 1050             


Ser Thr  Lys Glu Ile Leu Thr  Asp Gly Ser Gln Glu  Lys Gly Lys 
    1055                 1060                 1065             


Arg Thr  Asp Val Val Gly Tyr  Phe Thr Gly Ala Asp  Ile Ala Thr 
    1070                 1075                 1080             


Ala Ser  Ile Ala Ile Glu Gly  Pro Asp Arg Ser Phe  Ser Ala Arg 
    1085                 1090                 1095             


Gly Ile  Gly Phe Ala Ser Leu  Lys Asn Val Glu Lys  Leu Thr Val 
    1100                 1105                 1110             


Asn Tyr  Phe Gly Glu Leu His  Ser Val His Glu Thr  Gln Pro Met 
    1115                 1120                 1125             


Gln Phe  Arg Gly Gln Arg Gly  
    1130                 1135 


<210>  726
<211>  1136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  726

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asp Thr Gly Lys Ile Ile His Ala Ser Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Ser 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Asn Arg Arg Lys Lys His Arg Ser Val 
    50                  55                  60                  


Arg Leu Gln Asp Leu Phe Glu Ser Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Leu Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Gln Gly Met 
                85                  90                  95      


Glu Asn Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Thr Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Arg 
    130                 135                 140                 


Leu Leu Thr Glu Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Arg Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Asn Lys Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Thr Ile 
    290                 295                 300                 


Ile Glu Tyr Ala Lys Ser Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Lys Val Glu Glu Leu Pro 
        355                 360                 365             


Arg Lys Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Ser Lys Leu Lys Asp Thr Phe 
385                 390                 395                 400 


Asn Arg Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Leu Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Arg Ser Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asn Tyr Ile Lys Gln Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Asn Ile Phe His Gly His Met Glu 
545                 550                 555                 560 


Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Asn Ile Gln Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Glu Ser Lys Leu Leu Gly 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Lys Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Lys His 
    690                 695                 700                 


Gln Phe Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Lys Leu Glu Lys Ser Arg Asp Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys His Asn Asn Pro Leu Ile Ser Tyr Lys Asp Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Glu Thr Gly Glu Ile Val Ser Leu Ser Asn Glu Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Arg Ser Lys Lys Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Asp Lys Lys Glu Tyr Thr Tyr Thr Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ala Leu Gly Thr Lys Thr Pro Ser Lys Thr 
    850                 855                 860                 


Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Lys Thr Asp Lys Ser Gln Phe 
865                 870                 875                 880 


Leu Met Tyr Gln Lys Asp Arg Lys Thr Trp Asp Glu Val Ile Glu Lys 
                885                 890                 895     


Ile Ile Glu Gln Tyr Arg Pro Phe Lys Glu Tyr Asp Lys Asn Gly Lys 
            900                 905                 910         


Glu Val Asp Phe Asn Pro Phe Glu Lys Tyr Arg Ile Glu Asn Gly Pro 
        915                 920                 925             


Ile Arg Lys Tyr Ser Arg Lys Gly Asn Gly Ser Val Ile Lys Ser Met 
    930                 935                 940                 


Lys Tyr Tyr Asp Asn Leu Leu Gly Lys Phe Val Asp Ile Thr Pro Ser 
945                 950                 955                 960 


Glu Ser Lys Asn Pro Val Ala Leu Leu Ser Leu Asn Pro Trp Arg Thr 
                965                 970                 975     


Asp Val Tyr Tyr Asn Pro Glu Thr Arg Lys Tyr Glu Phe Leu Gly Leu 
            980                 985                 990         


Lys Tyr Ala Asp Leu Cys Phe Gly  Lys Gly Gly Ser Tyr  Gly Ile Ser 
        995                 1000                 1005             


Glu Val  Lys Tyr Asn Lys Ile  Arg Glu Lys Glu Gly  Ile Gly Lys 
    1010                 1015                 1020             


Glu Ser  Glu Phe Lys Phe Thr  Leu Tyr Lys Asn Asp  Leu Ile Leu 
    1025                 1030                 1035             


Ile Lys  Asp Thr Glu Thr Asn  Cys Gln Gln Phe Phe  Arg Phe Trp 
    1040                 1045                 1050             


Ser Arg  Thr Gly Lys Asp Asn  Pro Lys Ser Phe Glu  Lys His Lys 
    1055                 1060                 1065             


Ile Glu  Leu Lys Pro Tyr Glu  Lys Ala Lys Phe Glu  Lys Gly Glu 
    1070                 1075                 1080             


Glu Leu  Glu Val Leu Gly Lys  Val Pro Pro Ser Ser  Asn Gln Phe 
    1085                 1090                 1095             


Gln Lys  Asn Met Gln Ile Glu  Asn Leu Ser Ile Tyr  Lys Val Lys 
    1100                 1105                 1110             


Thr Asp  Val Leu Gly Asn Lys  His Phe Ile Lys Lys  Glu Gly Asp 
    1115                 1120                 1125             


Glu Pro  Lys Leu Lys Phe Lys  Lys 
    1130                 1135     


<210>  727
<211>  1136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  727

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Lys Lys Asp Thr Gly Glu Ile Ile His Thr Asn Ser 
            20                  25                  30          


Arg Leu Phe Ser Ala Ala Thr Ala Asp Ser Asn Ile Glu Arg Arg Arg 
        35                  40                  45              


His Arg Gly Gly Lys Arg Leu Thr Arg Arg Lys Lys His Arg Ser Ile 
    50                  55                  60                  


Arg Leu His Asp Leu Phe Glu Asp Ser Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Gln Gly Leu 
                85                  90                  95      


Asp Asn Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Thr Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Asp Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Thr Thr Ser Ala Tyr Ser Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Lys Lys Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asn Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Thr Thr Lys Lys Asp Pro Glu Gly Lys Tyr Ile Thr Leu Asp Asn 
                245                 250                 255     


Ile Phe Gly Ile Leu Ile Gly Lys Cys Thr Phe Tyr Pro Asp Glu Tyr 
            260                 265                 270         


Arg Ala Ser Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Glu 
    290                 295                 300                 


Glu Gln Lys Lys Thr Ile Ile Lys Tyr Ala Lys Thr Ala Lys Thr Leu 
305                 310                 315                 320 


Gly Ala Ser Thr Leu Leu Lys Tyr Ile Ala Lys Leu Ile Gly Ala Ser 
                325                 330                 335     


Val Asp Gln Ile His Gly Tyr Arg Ile Asp Pro Asn Lys Lys Pro Glu 
            340                 345                 350         


Met His Thr Phe Glu Thr Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile 
        355                 360                 365             


Ser Val Glu Glu Ile Pro Arg Lys Val Leu Asp Glu Leu Ala His Ile 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Arg Glu Gly Ile Glu Glu Ala Ile Asn Ala 
385                 390                 395                 400 


Thr Leu Lys Asp Thr Phe Ser Gln Asp Gln Val Leu Glu Leu Val Gln 
                405                 410                 415     


Phe Arg Lys Asn Asn Ser Ser Leu Phe Ser Lys Gly Trp His Asn Phe 
            420                 425                 430         


Ser Leu Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser 
        435                 440                 445             


Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys 
    450                 455                 460                 


Glu Thr Ser Lys Arg Thr Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu 
465                 470                 475                 480 


Glu Ile Tyr Asn Pro Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys 
                485                 490                 495     


Ile Ile Asn Glu Ala Thr Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val 
            500                 505                 510         


Ile Glu Met Ala Arg Glu Asn Asn Glu Glu Asp Ala Lys Lys Gly Tyr 
        515                 520                 525             


Asn Glu Arg Gln Lys Ala Asn Leu Asp Glu Lys Lys Ser Ala Met Glu 
    530                 535                 540                 


Lys Ala Ala Phe Gln Tyr Asn Gly Lys Lys Glu Leu Pro Asp Asn Val 
545                 550                 555                 560 


Phe His Gly His Lys Glu Leu Ala Thr Lys Ile Arg Leu Trp His Gln 
                565                 570                 575     


Gln Gly Glu Lys Cys Leu Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp 
            580                 585                 590         


Leu Ile Gln Asn Gln Tyr Lys Tyr Glu Ile Asp His Ile Leu Pro Leu 
        595                 600                 605             


Ser Leu Ser Phe Asp Asp Ser Leu Ser Asn Lys Val Leu Val Leu Ala 
    610                 615                 620                 


Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp 
625                 630                 635                 640 


Ser Met Asp Asp Ala Trp Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys 
                645                 650                 655     


Asp Leu Lys Leu Leu Ser Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu 
            660                 665                 670         


Glu Asp Ile Ser Lys Ile Glu Val Lys Gln Lys Phe Ile Glu Arg Asn 
        675                 680                 685             


Leu Val Asp Thr Arg Tyr Ser Ser Arg Val Val Leu Asn Ala Leu Gln 
    690                 695                 700                 


Asp Phe Tyr Lys Glu His Gln Phe Asp Thr Thr Ile Ser Val Val Arg 
705                 710                 715                 720 


Gly Gln Phe Thr Ser Gln Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser 
                725                 730                 735     


Arg Glu Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala Ala 
            740                 745                 750         


Ser Ser Gln Leu Arg Leu Trp Lys Lys Gln Asn Asn Pro Leu Ile Ser 
        755                 760                 765             


Tyr Lys Glu Gly Gln Phe Val Asp Gln Glu Thr Gly Glu Ile Ile Ser 
    770                 775                 780                 


Leu Ser Asp Asp Glu Tyr Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp 
785                 790                 795                 800 


His Phe Val Asp Thr Leu Lys Ser Lys Lys Phe Glu Asp Ser Ile Leu 
                805                 810                 815     


Phe Ser Tyr Gln Val Asp Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala 
            820                 825                 830         


Thr Ile Tyr Ala Thr Arg Lys Ala Lys Leu Asp Lys Glu Lys Lys Glu 
        835                 840                 845             


Tyr Thr Tyr Thr Leu Gly Lys Ile Lys Asp Ile Tyr Ala Leu Gly Ser 
    850                 855                 860                 


Glu Lys Leu Lys Thr Gly Phe Tyr Lys Phe Leu His Leu Tyr Lys Lys 
865                 870                 875                 880 


Asp Lys Ser Gln Phe Leu Met Tyr Gln Lys Asp Arg Lys Thr Trp Asp 
                885                 890                 895     


Glu Val Ile Glu Lys Ile Leu Glu Gln Tyr Arg Pro Phe Lys Glu Lys 
            900                 905                 910         


Asp Lys Asn Gly Lys Glu Val Asp Phe Asn Pro Phe Glu Lys Tyr Arg 
        915                 920                 925             


Ile Glu Asn Gly Pro Ile His Lys Tyr Ser Arg Lys Gly Asn Gly Pro 
    930                 935                 940                 


Val Ile Lys Ser Leu Lys Tyr Tyr Asp Lys Asn Ile Pro Glu Ser Phe 
945                 950                 955                 960 


Ile Asn Ile Thr Pro Ser Asn Ser Lys Asn Lys Val Ile Leu Arg Gln 
                965                 970                 975     


Leu Asn Pro Trp Arg Thr Asp Val Tyr Phe Asn His Gln Thr Glu Lys 
            980                 985                 990         


Tyr Glu Leu Leu Gly Leu Lys Tyr  Ala His Leu Gln Phe  Glu Lys Lys 
        995                 1000                 1005             


Thr Gly  Asn Tyr Ser Ile Thr  Ile Asp Lys Tyr Asn  Glu Ile Lys 
    1010                 1015                 1020             


Glu Lys  Glu Gly Val Ser Glu  Gln Ser Glu Phe Lys  Phe Thr Leu 
    1025                 1030                 1035             


His Lys  Asn Asp Leu Leu Leu  Ile Lys Asp Thr Glu  Thr Lys Glu 
    1040                 1045                 1050             


Gln Gln  Leu Phe Arg Phe Leu  Ser Arg Thr Lys Pro  Asn Val Lys 
    1055                 1060                 1065             


His Tyr  Val Glu Leu Lys Pro  Tyr Ser Arg Asp Glu  Phe Glu Lys 
    1070                 1075                 1080             


Asn Glu  Arg Leu Ile Glu Ile  Leu Gly Ser Val Asp  Lys Ser Gly 
    1085                 1090                 1095             


Arg Phe  Val Lys Gly Leu Gly  Lys Ser Asn Leu Ser  Ile Tyr Lys 
    1100                 1105                 1110             


Val Arg  Thr Asp Val Leu Gly  Asn Gln His Ile Ile  Lys Lys Glu 
    1115                 1120                 1125             


Gly Glu  Glu Pro Lys Leu Lys  Phe 
    1130                 1135     


<210>  728
<211>  1136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  728

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asp Thr Gly Lys Ile Ile His Ala Ser Ser 
            20                  25                  30          


Arg Leu Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Ser 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Asn Arg Arg Lys Lys His Arg Ser Val 
    50                  55                  60                  


Arg Leu Gln Asp Leu Phe Glu Gly Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Met Asn Leu Asn Pro Tyr Gln Leu Arg Val Gln Gly Met 
                85                  90                  95      


Glu Asn Gln Leu Thr Asn Glu Glu Leu Phe Val Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Thr Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Glu Asn Gly Glu 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Arg Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Ser Lys Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Ile Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Phe Arg Thr Asp Gly Thr Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
                245                 250                 255     


Gly Lys Cys Thr Phe Tyr Thr Glu Glu Tyr Arg Ala Ser Lys Ala Ser 
            260                 265                 270         


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
        275                 280                 285             


Val Pro Thr Glu Thr Lys Lys Leu Ser Glu Glu Gln Lys Lys Leu Ile 
    290                 295                 300                 


Ile Glu Tyr Ala Lys Ser Ala Lys Thr Leu Gly Ala Ser Thr Leu Leu 
305                 310                 315                 320 


Lys Tyr Ile Ala Lys Met Ile Asp Ala Ser Val Asp Gln Ile Arg Gly 
                325                 330                 335     


Tyr Arg Val Asp Val Asn Asn Lys Pro Glu Met His Thr Phe Glu Val 
            340                 345                 350         


Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile Lys Val Glu Glu Leu Pro 
        355                 360                 365             


Arg Lys Val Leu Asp Glu Leu Ala His Ile Leu Thr Leu Asn Thr Glu 
    370                 375                 380                 


Arg Glu Gly Ile Glu Glu Ala Ile Asn Ser Lys Leu Lys Asp Ile Phe 
385                 390                 395                 400 


Asn Arg Asp Gln Val Leu Glu Leu Val Gln Phe Arg Lys Asn Asn Ser 
                405                 410                 415     


Ser Leu Phe Ser Lys Gly Trp His Asn Phe Ser Ile Lys Leu Met Met 
            420                 425                 430         


Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr Ile 
        435                 440                 445             


Leu Thr Arg Leu Gly Lys Gln Arg Ser Lys Glu Thr Ser Lys Arg Thr 
    450                 455                 460                 


Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu Glu Ile Tyr Asn Pro Val 
465                 470                 475                 480 


Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Ile Asn Glu Ala Thr 
                485                 490                 495     


Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val Ile Glu Met Ala Arg Glu 
            500                 505                 510         


Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr Ile Lys Arg Gln Lys Ala 
        515                 520                 525             


Asn Gln Asp Glu Lys Asn Ala Ala Met Glu Lys Ala Ala Phe Gln Tyr 
    530                 535                 540                 


Asn Gly Lys Lys Glu Leu Pro Asp Asn Ile Phe His Gly His Lys Glu 
545                 550                 555                 560 


Leu Thr Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Lys Cys Leu 
                565                 570                 575     


Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp Leu Ile His Asn Gln Tyr 
            580                 585                 590         


Lys Tyr Glu Ile Asp His Ile Leu Pro Leu Ser Leu Ser Phe Asp Asp 
        595                 600                 605             


Ser Leu Ser Asn Lys Val Leu Val Leu Ala Thr Ala Asn Gln Glu Lys 
    610                 615                 620                 


Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp Ser Met Asp Asp Ala Trp 
625                 630                 635                 640 


Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys Asp Ser Lys Leu Leu Ser 
                645                 650                 655     


Asn Lys Lys Lys Asp Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys Ile 
            660                 665                 670         


Glu Val Lys Gln Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg Tyr 
        675                 680                 685             


Ser Ser Arg Val Val Leu Asn Ala Leu Gln Asp Phe Tyr Lys Ser His 
    690                 695                 700                 


Gln Leu Asp Thr Thr Ile Ser Val Val Arg Gly Gln Phe Thr Ser Gln 
705                 710                 715                 720 


Leu Arg Arg Lys Trp Gly Ile Glu Lys Ser Arg Glu Thr Tyr His His 
                725                 730                 735     


His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Arg Leu 
            740                 745                 750         


Trp Lys Lys His Ser Asn Pro Leu Ile Ala Tyr Lys Glu Gly Gln Phe 
        755                 760                 765             


Val Asp Ser Glu Thr Gly Glu Ile Val Ser Leu Ser Asp Glu Glu Tyr 
    770                 775                 780                 


Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp His Phe Val Asp Thr Leu 
785                 790                 795                 800 


Arg Ser Lys Lys Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp 
                805                 810                 815     


Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg 
            820                 825                 830         


Lys Ala Lys Leu Asp Lys Glu Lys Lys Glu Tyr Thr Tyr Thr Leu Gly 
        835                 840                 845             


Lys Ile Lys Asp Ile Tyr Ala Leu Gly Thr Lys Thr Pro Ser Lys Thr 
    850                 855                 860                 


Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Lys Thr Asp Lys Ser Gln Phe 
865                 870                 875                 880 


Leu Met Tyr Gln Lys Asp Arg Lys Thr Trp Asp Glu Val Ile Glu Lys 
                885                 890                 895     


Ile Ile Glu Gln Tyr Arg Pro Phe Lys Glu Tyr Asp Lys Asn Gly Lys 
            900                 905                 910         


Glu Val Asp Phe Asn Pro Phe Glu Lys Tyr Arg Ile Gly Asn Gly Pro 
        915                 920                 925             


Ile Arg Lys Tyr Ser Lys Lys Gly Asn Gly Pro Glu Ile Lys Ser Leu 
    930                 935                 940                 


Lys Tyr Tyr Asp Ile Leu Leu Gly Lys His Lys Asn Ile Thr Pro Asp 
945                 950                 955                 960 


Gly Ser Arg Asn Thr Val Ala Leu Leu Ser Leu Asn Pro Trp Arg Thr 
                965                 970                 975     


Asp Val Tyr Tyr Asn Ser Glu Thr Lys Lys Tyr Glu Phe Leu Gly Leu 
            980                 985                 990         


Lys Tyr Ala Asp Leu Cys Phe Glu  Glu Gly Gly Ala Tyr  Gly Ile Ser 
        995                 1000                 1005             


Glu Val  Lys Tyr Lys Lys Ile  Arg Glu Lys Glu Gly  Ile Gly Lys 
    1010                 1015                 1020             


Asn Ser  Glu Phe Lys Phe Thr  Leu Tyr Lys Asn Asp  Leu Ile Leu 
    1025                 1030                 1035             


Ile Lys  Asp Thr Glu Thr Asn  Cys Gln Gln Phe Phe  Arg Phe Trp 
    1040                 1045                 1050             


Ser Arg  Thr Gly Lys Asp Asn  Pro Lys Ser Phe Glu  Lys His Lys 
    1055                 1060                 1065             


Ile Glu  Leu Lys Pro Tyr Glu  Lys Ala Lys Phe Glu  Lys Gly Glu 
    1070                 1075                 1080             


Glu Leu  Lys Val Leu Gly Lys  Val Pro Pro Ser Ser  Asn Gln Phe 
    1085                 1090                 1095             


Gln Lys  Asn Met Gln Ile Glu  Asn Leu Ser Ile Tyr  Lys Val Lys 
    1100                 1105                 1110             


Thr Asp  Ile Leu Gly Asn Lys  His Phe Ile Lys Lys  Glu Gly Asp 
    1115                 1120                 1125             


Glu Pro  Lys Leu Lys Phe Lys  Lys 
    1130                 1135     


<210>  729
<211>  1139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  729

Met Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Glu Arg Arg Thr 
        35                  40                  45              


Asn Arg Gln Gly Arg Arg Leu Thr Arg Arg Lys Lys His Arg Arg Val 
    50                  55                  60                  


Arg Leu Asn His Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
                85                  90                  95      


Thr Ala Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
            100                 105                 110         


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
        115                 120                 125             


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
    130                 135                 140                 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Glu Asp Gly Arg 
                165                 170                 175     


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr His Ala Glu 
            180                 185                 190         


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Asn Ser Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Lys 
225                 230                 235                 240 


Tyr Thr Thr Lys Lys Asp Ala Gln Gly Gln Tyr Ile Thr Leu Asn Asn 
                245                 250                 255     


Ile Phe Gly Ile Leu Ile Gly Lys Cys Thr Phe Tyr Pro Glu Glu Tyr 
            260                 265                 270         


Arg Ala Ala Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Glu 
    290                 295                 300                 


Glu Gln Lys Tyr Gln Ile Ile Thr Tyr Val Lys Asn Glu Lys Ala Met 
305                 310                 315                 320 


Gly Pro Ala Lys Leu Phe Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp 
                325                 330                 335     


Val Ala Asp Ile Lys Gly Tyr Arg Ile Asp Lys Ser Asp Lys Ala Glu 
            340                 345                 350         


Ile His Thr Phe Glu Ala Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu 
        355                 360                 365             


Asp Val Lys Lys Met Ala Arg Glu Glu Leu Asp Lys Leu Ala Tyr Val 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Arg Glu Gly Ile Gln Glu Ala Leu Asp His 
385                 390                 395                 400 


Glu Phe Ala Asp Gly Thr Phe Ser Gln Glu Gln Val Asp Glu Leu Val 
                405                 410                 415     


Gln Phe Arg Lys Ala Asn Ser Ser Ile Phe Gly Lys Gly Trp His Ser 
            420                 425                 430         


Phe Ser Val Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Ala Thr 
        435                 440                 445             


Ser Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr 
    450                 455                 460                 


Thr Ser Ser Ser Asn Lys Thr Lys Tyr Ile Asp Glu Lys Gln Leu Thr 
465                 470                 475                 480 


Glu Glu Ile Tyr Asn Pro Val Val Ala Lys Ser Val Arg Gln Ala Ile 
                485                 490                 495     


Lys Ile Val Asn Ala Ala Ile Lys Lys Tyr Gly Asp Phe Asp Asn Ile 
            500                 505                 510         


Val Ile Glu Met Ala Arg Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala 
        515                 520                 525             


Ile Gln Lys Ile Gln Lys Ala Asn Lys Ala Glu Lys Asp Ala Ala Met 
    530                 535                 540                 


Arg Lys Ala Ala Asn Gln Tyr Asn Gly Lys Ala Glu Leu Pro His Ser 
545                 550                 555                 560 


Val Phe His Gly His Lys Gln Leu Ala Thr Lys Ile Arg Leu Trp His 
                565                 570                 575     


Gln Gln Gly Glu Arg Cys Leu Tyr Thr Gly Lys Thr Ile Ser Ile His 
            580                 585                 590         


Asp Leu Ile Asn Asn Pro Asn Gln Phe Glu Ile Asp His Ile Leu Pro 
        595                 600                 605             


Leu Ser Ile Thr Phe Asp Asp Ser Leu Ala Asn Lys Val Leu Val Tyr 
    610                 615                 620                 


Ala Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu 
625                 630                 635                 640 


Asp Ser Met Asp Asp Ala Trp Ser Phe Arg Glu Leu Lys Ala Phe Val 
                645                 650                 655     


Arg Glu Ser Lys Ala Leu Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr 
            660                 665                 670         


Glu Glu Asp Ile Ser Lys Phe Asp Val Arg Lys Lys Phe Ile Glu Arg 
        675                 680                 685             


Asn Leu Val Asp Thr Arg Tyr Ala Ser Arg Val Val Leu Asn Ala Leu 
    690                 695                 700                 


Gln Glu His Phe Arg Thr His Lys Ile Asp Thr Lys Val Ser Val Val 
705                 710                 715                 720 


Arg Gly Gln Phe Thr Ser Gln Leu Arg Arg His Trp Gly Ile Glu Lys 
                725                 730                 735     


Thr Arg Asp Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala 
            740                 745                 750         


Ala Ser Ser Gln Leu Asn Leu Trp Lys Lys Gln Lys Asn Thr Leu Val 
        755                 760                 765             


Ser Tyr Ser Glu Asp Gln Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile 
    770                 775                 780                 


Ser Asp Asp Glu Tyr Lys Glu Ser Val Phe Lys Ala Pro Tyr Gln His 
785                 790                 795                 800 


Phe Val Asp Thr Leu Lys Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe 
                805                 810                 815     


Ser Tyr Gln Val Asp Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr 
            820                 825                 830         


Ile Tyr Ala Thr Arg Lys Ala Lys Leu Asp Lys Glu Lys Lys Glu Tyr 
        835                 840                 845             


Thr Tyr Thr Leu Gly Lys Ile Lys Asp Ile Tyr Ala Leu Gly Thr Lys 
    850                 855                 860                 


Thr Pro Ser Lys Thr Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Lys Lys 
865                 870                 875                 880 


Asp Lys Ser Gln Phe Leu Met Tyr Gln Lys Asp Arg Arg Thr Trp Asp 
                885                 890                 895     


Glu Val Ile Glu Lys Ile Leu Glu Gln Tyr Arg Pro Phe Lys Glu Lys 
            900                 905                 910         


Asp Lys Asn Gly Lys Glu Val Asp Phe Asn Pro Phe Glu Lys Tyr Arg 
        915                 920                 925             


Ile Glu Asn Gly Pro Ile Arg Lys Tyr Ser Arg Lys Gly Asn Gly Pro 
    930                 935                 940                 


Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Asn Leu Leu Gly Arg Phe Val 
945                 950                 955                 960 


Asp Ile Thr Pro Ser Glu Ser Lys Asn Pro Val Ala Leu Leu Ser Leu 
                965                 970                 975     


Asn Pro Trp Arg Thr Asp Val Tyr Tyr Asn Thr Glu Thr Arg Lys Tyr 
            980                 985                 990         


Glu Phe Leu Gly Leu Lys Tyr Ala  Asp Leu Cys Phe Glu  Lys Gly Gly 
        995                 1000                 1005             


Ser Tyr  Gly Ile Ser Lys Val  Lys Tyr Asn Lys Ile  Arg Glu Lys 
    1010                 1015                 1020             


Glu Gly  Ile Gly Lys Asn Ser  Glu Phe Lys Phe Thr  Leu Tyr Lys 
    1025                 1030                 1035             


Asn Asp  Leu Ile Leu Ile Lys  Asp Thr Glu Thr Asn  Arg Gln Gln 
    1040                 1045                 1050             


Ile Phe  Arg Phe Trp Ser Arg  Thr Gly Lys Asp Asn  Pro Lys Ser 
    1055                 1060                 1065             


Phe Glu  Lys His Lys Leu Glu  Leu Lys Pro Tyr Glu  Lys Thr Arg 
    1070                 1075                 1080             


Phe Glu  Lys Gly Glu Glu Leu  Lys Val Leu Gly Lys  Val Pro Pro 
    1085                 1090                 1095             


Ser Ser  Asn Arg Leu Gln Lys  Asn Met Gln Ile Glu  Asn Leu Ser 
    1100                 1105                 1110             


Ile Tyr  Lys Val Arg Thr Asp  Val Leu Gly Asn Gln  His Ile Ile 
    1115                 1120                 1125             


Lys Asn  Glu Gly Asp Lys Pro  Lys Leu Asp Phe 
    1130                 1135                 


<210>  730
<211>  1139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  730

Met Asn Tyr Tyr Asn Gly Phe Ile Ile Thr Glu Asn Ala Met Ala Ser 
1               5                   10                  15      


Lys His Glu Asn Leu Gly Ala Leu Thr Phe Gly Leu Asp Ile Gly Ile 
            20                  25                  30          


Ala Ser Val Gly Trp Ala Val Leu Gly Glu Asn Arg Ile Ile Asp Leu 
        35                  40                  45              


Gly Val Arg Cys Phe Asp Lys Ala Glu Thr Ala Lys Glu Gly Glu Ser 
    50                  55                  60                  


Leu Asn Leu Ala Arg Arg Thr Ala Arg Leu Leu Arg Arg Arg Leu Arg 
65                  70                  75                  80  


Arg Arg Ala Trp Arg Leu Thr Lys Leu Ala Arg Leu Leu Lys Arg Glu 
                85                  90                  95      


Gly Leu Ile Ala Asp Thr Asn Val Phe Gln Lys Pro Phe Asn Leu Lys 
            100                 105                 110         


Asp Ala Asn Glu Lys Gly Leu Ser Phe Trp Gln Leu Arg Val Asp Ala 
        115                 120                 125             


Leu Asp Arg Lys Leu Ser Ala Glu Glu Trp Ala Arg Val Ile Tyr His 
    130                 135                 140                 


Leu Cys Lys His Arg Gly Phe His Trp Ile Ser Arg Ala Glu Glu Lys 
145                 150                 155                 160 


Ala Ala Glu Gly Asp Ser Lys Ser Glu Gly Gly Lys Val Lys Gln Gly 
                165                 170                 175     


Leu Ala Gly Thr Lys Lys Leu Met Gln Glu Lys Asn Tyr Arg Thr Ala 
            180                 185                 190         


Ala Glu Met Val Leu Thr Glu Phe Pro Glu Ala Gln Arg Asn Lys Gln 
        195                 200                 205             


Gly Asp Tyr Ser Lys Ala Leu Ser Arg Ile Leu Leu Gly Asp Glu Met 
    210                 215                 220                 


Thr Gln Leu Phe Lys His Gln Arg Glu Leu Gly Asn Pro His Ala Thr 
225                 230                 235                 240 


Glu Lys Leu Glu Ala Ala Ile Leu Gly Asn Gly Asp His Lys Ser Gly 
                245                 250                 255     


Leu Phe Trp Ala Gln Asn Pro Ala Leu Ala Gly Asn Asp Leu Leu Lys 
            260                 265                 270         


Met Leu Gly His Cys Thr Phe Glu Lys Ala Glu Tyr Arg Val Pro Lys 
        275                 280                 285             


Ala Ser Phe Thr Ala Glu Arg His Val Trp Leu Thr Arg Leu Asn Asn 
    290                 295                 300                 


Leu Arg Ile Val Val Asp Gly Thr Thr Arg Pro Leu Asn Glu Ala Glu 
305                 310                 315                 320 


Arg His Leu Ala Leu Leu Met Pro Tyr Gln Gln Ala Gly Asp Leu Thr 
                325                 330                 335     


Tyr Lys Gln Leu Arg Thr Ala Leu Thr Lys Ala Gly Tyr Leu Pro Glu 
            340                 345                 350         


Ser Phe Arg Tyr Thr Gly Leu Ala Tyr Ala Ser Gly Gln Lys Ala Asp 
        355                 360                 365             


Glu Lys Ala Lys Asp Pro Glu Ser Ala Pro Leu Val Lys Leu Pro Ala 
    370                 375                 380                 


Trp His Glu Leu Arg Lys Thr Leu Glu Lys Lys Glu Leu Ala Ser Glu 
385                 390                 395                 400 


Trp Gln Lys Ile Ser Ser Asp Ala Leu Asp Gly Lys Pro Gln Leu Leu 
                405                 410                 415     


Asp Gln Ile Ala Trp Val Leu Ser Val Tyr Lys Asp Asp Thr Glu Val 
            420                 425                 430         


Glu Ala Glu Leu Cys Lys Leu Asn Leu Pro Asn Ala Glu Lys Met Ile 
        435                 440                 445             


Asp Ala Leu Leu Asp Ile Arg Phe Asp Lys Phe Ser Asn Leu Ser Leu 
    450                 455                 460                 


Lys Ala Leu Arg Gln Ile Val Pro Leu Met Glu Gln Gly Gln Arg Tyr 
465                 470                 475                 480 


Asp Glu Ala Val Ala Asn Ile Pro Glu Tyr Gly His His Ser Gln Leu 
                485                 490                 495     


His Glu Val Gly Ala Gly Glu His Lys Tyr Leu Pro Pro Phe Tyr Lys 
            500                 505                 510         


Glu Arg Asp Lys Thr Gly Arg Met Val Phe Asn Asp Asp Met Asp Ile 
        515                 520                 525             


Pro Arg Asn Pro Val Val Leu Arg Ala Leu Asn Gln Ala Arg Lys Val 
    530                 535                 540                 


Val Asn Ala Leu Ile Lys Glu Tyr Gly Ser Pro His Glu Val His Ile 
545                 550                 555                 560 


Glu Met Ala Arg Asp Leu Ser Arg Pro Leu Asp Glu Arg Arg Asp Val 
                565                 570                 575     


Glu Lys Leu Gln Lys Glu Phe Arg Asp Arg Asn Glu Lys Asp Lys Ala 
            580                 585                 590         


Leu Phe Ala Gln Asp Phe Asn Ile Val Gly Ala Val Lys Gly Lys Glu 
        595                 600                 605             


Phe Glu Lys Tyr Gln Leu Tyr Arg Glu Gln Gln Gly Lys Cys Ala Tyr 
    610                 615                 620                 


Ser Ile Glu Pro Met Asp Ile His Arg Leu Phe Glu Gln Gly Tyr Val 
625                 630                 635                 640 


Glu Ile Asp His Ala Leu Pro Tyr Ser Arg Ser Phe Asp Asp Ser Lys 
                645                 650                 655     


Asn Asn Arg Val Leu Val Leu Ala Lys Glu Asn Arg Asp Lys Gly Asn 
            660                 665                 670         


Arg Thr Pro Phe Glu Tyr Leu Gly Gly Lys Asp Asn Ser Glu Arg Trp 
        675                 680                 685             


Arg Gln Phe Val Ala Phe Val Glu Gly Asn Lys Ser Tyr Arg Leu Ala 
    690                 695                 700                 


Lys Arg Ser Arg Leu Leu Arg Lys Asp Phe Gly Lys Asp Glu Ala Lys 
705                 710                 715                 720 


Gly Phe Arg Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ile Cys Arg Phe 
                725                 730                 735     


Phe Lys Asn Tyr Val Glu Gln Tyr Leu Gln Leu His Glu Asp Ser Asp 
            740                 745                 750         


Ala Lys Arg Cys Val Val Leu Ser Gly Gln Met Thr Ser Phe Leu Arg 
        755                 760                 765             


Ala Arg Trp Gly Leu Val Lys Val Arg Gly Asp Ser Asp Arg His His 
    770                 775                 780                 


Ala Leu Asp Gly Ala Ile Val Ala Ala Cys Ser His Ser Met Val Lys 
785                 790                 795                 800 


Arg Leu Ser Asp Tyr Ser Arg Arg Lys Glu Leu Glu Gln Val Arg Ala 
                805                 810                 815     


Gly Phe Val Asp Val Asp Thr Gly Glu Ile Val Asn Pro Ala Met Phe 
            820                 825                 830         


Gln Gln Val Gln Glu His Phe Pro Asn Pro Trp Pro His Phe Arg Asp 
        835                 840                 845             


Glu Val Lys Leu Arg Leu Asn Val Asp Asp Pro Ala Leu Leu His Ser 
    850                 855                 860                 


Glu Met Asn Arg Phe Gly Thr Tyr Ser Pro Glu Ala Leu Lys Ala Leu 
865                 870                 875                 880 


Arg Pro Leu Phe Ile Ser Arg Ala Pro Gln Arg Arg Asn Ser Gly Ala 
                885                 890                 895     


Ala His Lys Asp Thr Ile Tyr Ala Gln Pro Glu Arg Leu Val Ala Gln 
            900                 905                 910         


Gly Gly Val Thr Gln Lys Val Pro Leu Thr Ser Leu Thr Leu Lys Asp 
        915                 920                 925             


Leu Asp Lys Leu Ile Asp Pro His Arg Asn Glu Lys Leu Tyr Ser Ala 
    930                 935                 940                 


Ile Arg Ile Arg Leu Glu Gln His Gly Gly Lys Gly Asp Lys Ala Phe 
945                 950                 955                 960 


Pro Ala Ser Asn Pro Leu Arg Lys Pro Asp Ser Glu Gly Asn Pro Thr 
                965                 970                 975     


Gly Pro Ile Val Arg Gly Val Thr Met Val Ile Asp Lys Leu Ser Gly 
            980                 985                 990         


Ile Pro Ile Arg Gly Gly Ile Ala  Lys Asn Asp Ser Met  Val Arg Val 
        995                 1000                 1005             


Asp Val  Phe Thr Lys Ala Gly  Lys Phe His Leu Val  Pro Val Tyr 
    1010                 1015                 1020             


Val His  His Ala Val Ala Lys  Val Leu Pro Asn Arg  Ala Val Val 
    1025                 1030                 1035             


Ala Tyr  Lys Asp Glu Thr Glu  Trp Thr Leu Ile Asp  Asp Gly Phe 
    1040                 1045                 1050             


Asp Phe  Cys Phe Ser Met Tyr  Pro Asn Asp Leu Leu  Arg Ile Lys 
    1055                 1060                 1065             


Leu Arg  Asn Ala Ser His Leu  Gly Tyr Tyr Ala Ser  Cys Asn Arg 
    1070                 1075                 1080             


Ser Thr  Gly Ala Ile Asn Leu  Trp Ser His Asp Arg  Asn Gln Gln 
    1085                 1090                 1095             


Val Gly  Lys Asp Gly Met Ile  Glu Ser Ile Gly Val  Lys Thr Ala 
    1100                 1105                 1110             


Leu Asn  Val Glu Lys Leu Asn  Ile Asp Val Leu Gly  Asn Arg Tyr 
    1115                 1120                 1125             


Pro Ala  Pro Pro Glu Lys Arg  Arg Asp Leu Ala 
    1130                 1135                 


<210>  731
<211>  1140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  731

Met Lys Asn Met Thr Lys Val Asn Val Gly Leu Asp Val Gly Ile Ser 
1               5                   10                  15      


Ser Ala Gly Trp Ser Val Ile Asn Ala Glu Thr Asn Gln Ile Leu Glu 
            20                  25                  30          


Ser Gly Val Arg Leu Phe Ser Gly Ser Asp Ala Asp Glu Asn Val Asn 
        35                  40                  45              


Arg Arg Asn Gln Arg Gln Gly Arg Arg Leu Phe Arg Arg Arg Lys Leu 
    50                  55                  60                  


Arg Ile Gln Glu Ala Lys Lys Tyr Leu Glu Asn Val Phe Gln Leu Lys 
65                  70                  75                  80  


Asp Ile Glu Pro Lys Asp Glu Trp Asn Pro Tyr Glu Ile Arg Val Arg 
                85                  90                  95      


Ala Leu Asn Glu Lys Val Ser Lys Glu Glu Leu Ser Ile Ala Leu Leu 
            100                 105                 110         


Asn Ile Val Lys His Arg Gly Ile Ser Tyr Glu Leu Glu Asp Val Thr 
        115                 120                 125             


Asp Glu Glu Gly Thr Asn Thr Gln Ser Phe Gln Ser Gly Leu Asp Tyr 
    130                 135                 140                 


Asn Arg Arg Leu Leu Lys Thr Lys Thr Val Gly Glu Ile Gln Tyr Asp 
145                 150                 155                 160 


Arg Leu Gln Lys Tyr Gly Gln Val Arg Gly Gln Ile Gln Thr Glu Asp 
                165                 170                 175     


Gly Leu Leu Ile Asn Ile Phe Pro Thr Lys Asn Tyr Val Glu Glu Val 
            180                 185                 190         


Glu Arg Ile Leu Glu Thr Gln Lys Lys Tyr His Pro Glu Ile Asp Asp 
        195                 200                 205             


Val Phe Ile Glu Lys Ile Ile Ser Phe Val Thr Arg Lys Arg Glu Tyr 
    210                 215                 220                 


Phe Val Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Ile Tyr 
225                 230                 235                 240 


Arg Glu Asn Gly Glu Thr Leu Asp Asn Leu Phe Glu Ile Leu Ile Gly 
                245                 250                 255     


Lys Asp Lys Phe Tyr Pro Glu Glu Tyr Arg Ala Ala Gly Ser Ser Tyr 
            260                 265                 270         


Thr Ala Gln Ile Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Pro Thr Thr Glu Asp His His Leu Thr Lys Ala Gln Lys Glu Glu Ile 
    290                 295                 300                 


Ile Thr Glu Leu Lys Asn Thr Ser Arg Leu Ser Gly Gly Met Leu Lys 
305                 310                 315                 320 


Leu Ile Ser Lys Val Thr Lys Ala Pro Lys Glu Glu Ile Ser Gly Tyr 
                325                 330                 335     


Arg Lys Asp Glu Lys Asp Lys Pro Asp Leu His Ser Leu Ala Tyr Tyr 
            340                 345                 350         


Arg Lys Gln Lys Asn Met Leu Glu Lys Glu Gly Ile Gln Ile Ala Asp 
        355                 360                 365             


Phe Ser Glu Glu Leu Leu Asp Glu Leu Ala Thr Met Met Thr Leu Asn 
    370                 375                 380                 


Thr Glu Pro Gly Glu Ile Arg Lys Gln Leu Asn Lys Ile Thr Asn Gln 
385                 390                 395                 400 


Tyr Asp Ile Leu Thr Pro Glu Val Ile Glu Val Leu Val Lys Asn His 
                405                 410                 415     


Thr Ala Phe Val Ala Thr Ser Asn His Lys Trp His Arg Phe Ser Leu 
            420                 425                 430         


Lys Leu Met Lys Glu Phe Ile Pro Glu Leu Leu Glu Thr Ser Lys Glu 
        435                 440                 445             


Gln Ser Thr Ile Leu Cys Glu Arg Gly Phe Ile Gln Asn Arg Ala Ser 
    450                 455                 460                 


Tyr Leu Asp Glu Asn His His Phe Asn Tyr Ala Glu Leu Arg Glu Asn 
465                 470                 475                 480 


Leu Tyr Asn Pro Val Val Arg Lys Ser Thr Cys Glu Met Leu Lys Val 
                485                 490                 495     


Phe Thr Ala Ile Gln Glu Lys Tyr Pro Asn Ile Gln Asn Val Ile Val 
            500                 505                 510         


Glu Leu Pro Arg Glu Thr Asn Glu Asp Glu Gln Lys Arg Lys Ile Lys 
        515                 520                 525             


Lys Ile Asn Asp Asn Asn Ala Lys Arg Lys Lys Glu Ile Leu Lys Glu 
    530                 535                 540                 


Ala Gln Ala Gln Ser Gly Leu Ser Glu Glu Ala Leu Glu Glu Lys Leu 
545                 550                 555                 560 


Leu Lys Thr Lys Gly Leu Tyr Leu Lys Leu Met Leu Trp Tyr Glu Gln 
                565                 570                 575     


Glu Gly Lys Cys Val Tyr Ser Gly Gln Glu Ile Pro Leu Ser Asp Leu 
            580                 585                 590         


Leu Gln Asn Lys Gly Gly Tyr Glu Val Asp His Ile Ile Pro Ile Ser 
        595                 600                 605             


Ile Ser Phe Asp Asp Arg Leu Cys Asn Lys Val Leu Val Arg Gln Gln 
    610                 615                 620                 


Leu Asn Ser Gln Lys Ser Asn Gln Thr Pro Phe Glu Tyr Met Thr Thr 
625                 630                 635                 640 


His Thr Asp Gly His Leu Ser Phe His Glu Leu Lys Glu Leu Val Gly 
                645                 650                 655     


Lys Asn Lys Arg Phe Ser Lys Glu Lys Arg Glu Leu Leu Leu Ala Glu 
            660                 665                 670         


Ile Tyr Ser Asn Asp Ile Glu Thr Arg Lys Arg Phe Ile Asn Arg Asn 
        675                 680                 685             


Leu Val Asp Thr Ser Tyr Thr Ala Arg Ile Val Leu Asn Ala Leu Gln 
    690                 695                 700                 


Gln Tyr Lys Lys Glu Asn Gln Leu Ser Met Asn Val Ser Val Val Arg 
705                 710                 715                 720 


Gly Ala Met Thr Asn Ile Leu Arg Lys Lys Val Lys Leu Asp Lys Thr 
                725                 730                 735     


Arg Glu Thr Phe His His His Ala Ile Asp Ala Ser Leu Met Ala Val 
            740                 745                 750         


Ile Pro Asn Ile Glu Phe Phe Lys Glu Arg Lys Glu Trp Leu Pro Lys 
        755                 760                 765             


Ser Ala Asn Glu Glu Val Val Glu Val Glu Val Ser Glu Glu Glu Thr 
    770                 775                 780                 


Met Pro Glu Lys Glu Phe Lys Val Lys Met Arg Leu Pro Glu Ser Phe 
785                 790                 795                 800 


Ile Gln Asp Val Gln Ile Leu Gln Arg Asn Ser Phe Ala Lys Glu Glu 
                805                 810                 815     


Val Ser Gly Pro Gln Thr Arg Glu Arg Asn Leu Lys Ile Lys Phe Ser 
            820                 825                 830         


His Gln Val Asp Gln Lys Met Asn Arg Lys Val Ala Asp Asp Thr Ile 
        835                 840                 845             


Tyr Gly Thr Arg Gln Ala Gln Leu Gly Lys Asp Lys Lys Glu Glu Thr 
    850                 855                 860                 


Tyr Val Ile Gly Lys Ile Lys Asp Ile Tyr Asn Tyr Asp Glu Tyr Val 
865                 870                 875                 880 


Arg Phe Lys Lys Arg Tyr Asp Lys Asn Lys Glu Ser Phe Leu Met Tyr 
                885                 890                 895     


His His Asp Pro Gln Thr Phe Glu Lys Leu Glu Glu Ile Leu Ala Gln 
            900                 905                 910         


Tyr Pro Glu Tyr Ile Glu Asp Glu Lys Gly Lys Lys Lys Lys Val Ser 
        915                 920                 925             


Pro Phe Ala Leu Tyr Arg Glu Glu Asn Gly Pro Val Thr Lys Tyr Ala 
    930                 935                 940                 


Lys Lys Asn Asn Gly Pro Val Ile Lys Gln Leu Lys Tyr Tyr Asp Ser 
945                 950                 955                 960 


Lys Leu Asn Arg Lys Ile Asp Ile Ser His Lys Tyr Ser Thr Lys Asn 
                965                 970                 975     


Arg Gln Val Val Leu Gln Ser Leu Lys Pro Tyr Arg Thr Asp Val Tyr 
            980                 985                 990         


Arg Asn Gln Asp Thr Gly Glu Tyr  Thr Leu Val Gly Ile  Asp Gln Asn 
        995                 1000                 1005             


Asp Gly  Lys Phe Val Asn Gly  His Tyr Gly Ile Pro  Lys Ser Arg 
    1010                 1015                 1020             


Tyr Glu  Glu Ile Lys Ala Asn  Asn Lys Leu Ser Glu  His Asp Glu 
    1025                 1030                 1035             


Phe Val  Phe Ser Leu Tyr Lys  Asn Ser Met Val Lys  Val Ile Asp 
    1040                 1045                 1050             


Glu Asn  Gly Glu Ser Ile Val  Val Arg Tyr Leu Ser  Arg Ser Met 
    1055                 1060                 1065             


Pro Asn  Arg Lys Asn Asn Ala  Glu Ile Lys Pro Val  Glu Lys Tyr 
    1070                 1075                 1080             


Gln Phe  Glu Asp Gly Glu Val  Val Pro Val Tyr Gly  Lys Cys Arg 
    1085                 1090                 1095             


Lys Gln  Met Gln Thr Val Phe  Val Lys Lys Gly Trp  Thr Leu Leu 
    1100                 1105                 1110             


Lys Gly  Asn Thr Asp Ile Leu  Gly Asn Thr Tyr Phe  Val Glu Lys 
    1115                 1120                 1125             


Glu Ser  Glu Asn Pro Lys Lys  Met Ile Asp Asp Lys  
    1130                 1135                 1140 


<210>  732
<211>  1140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  732

Met Arg Glu Asn Gly Ser Asp Glu Arg Arg Arg Asn Met Asp Glu Lys 
1               5                   10                  15      


Met Asp Tyr Arg Ile Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Trp 
            20                  25                  30          


Ala Val Leu Gln Asn Asn Ser Asp Asp Glu Pro Val Arg Ile Val Asp 
        35                  40                  45              


Leu Gly Val Arg Ile Phe Asp Thr Ala Glu Ile Pro Lys Thr Gly Glu 
    50                  55                  60                  


Ser Leu Ala Gly Pro Arg Arg Ala Ala Arg Thr Thr Arg Arg Arg Leu 
65                  70                  75                  80  


Arg Arg Arg Lys His Arg Leu Asp Arg Ile Lys Trp Leu Phe Glu Asn 
                85                  90                  95      


Gln Gly Leu Ile Asn Ile Asp Asp Phe Leu Lys Arg Tyr Asn Met Ala 
            100                 105                 110         


Gly Leu Pro Asp Val Tyr Gln Leu Arg Tyr Glu Ala Leu Asp Arg Lys 
        115                 120                 125             


Leu Thr Asp Glu Glu Leu Ala Gln Val Leu Leu His Ile Ala Lys His 
    130                 135                 140                 


Arg Gly Phe Arg Ser Thr Arg Lys Ala Glu Thr Ala Ala Lys Glu Asn 
145                 150                 155                 160 


Gly Ala Val Leu Lys Ala Thr Asp Glu Asn Gln Lys Arg Met Gln Glu 
                165                 170                 175     


Lys Gly Tyr Arg Thr Val Gly Glu Met Ile Tyr Leu Asp Glu Ala Phe 
            180                 185                 190         


Arg Thr Gly Cys Ser Trp Ser Glu Lys Gly Tyr Ile Leu Thr Pro Arg 
        195                 200                 205             


Asn Lys Ala Glu Asn Tyr Gln His Thr Met Leu Arg Ala Met Leu Val 
    210                 215                 220                 


Glu Glu Val Lys Glu Ile Phe Ser Ser Gln Arg Arg Leu Gly Asn Glu 
225                 230                 235                 240 


Lys Ala Thr Glu Glu Leu Glu Glu Lys Tyr Leu Glu Ile Met Thr Ser 
                245                 250                 255     


Gln Arg Ser Phe Asp Leu Gly Pro Gly Met Gln Pro Asp Gly Lys Pro 
            260                 265                 270         


Ser Pro Tyr Ala Met Glu Gly Phe Ser Asp Arg Val Gly Lys Cys Thr 
        275                 280                 285             


Phe Leu Gly Asp Gln Gly Glu Leu Arg Gly Ala Lys Gly Thr Tyr Thr 
    290                 295                 300                 


Ala Glu Tyr Phe Val Ala Leu Gln Lys Ile Asn His Thr Lys Leu Val 
305                 310                 315                 320 


Asn Gln Asp Gly Glu Thr Arg Asn Phe Thr Glu Glu Glu Arg Arg Ala 
                325                 330                 335     


Leu Thr Leu Leu Leu Phe Thr Gln Lys Glu Val Lys Tyr Ala Ala Val 
            340                 345                 350         


Arg Lys Lys Leu Gly Leu Pro Glu Asp Ile Leu Phe Tyr Asn Leu Asn 
        355                 360                 365             


Tyr Lys Lys Ala Ala Thr Lys Glu Glu Gln Gln Lys Glu Asn Gln Asn 
    370                 375                 380                 


Thr Glu Lys Ala Lys Phe Ile Gly Met Pro Tyr Tyr His Asp Tyr Lys 
385                 390                 395                 400 


Lys Cys Leu Glu Glu Arg Val Lys Tyr Leu Thr Glu Asn Glu Val Arg 
                405                 410                 415     


Asp Leu Phe Asp Glu Ile Gly Met Ile Leu Thr Cys Tyr Lys Asn Asp 
            420                 425                 430         


Asp Ser Arg Thr Glu Arg Leu Ala Lys Leu Gly Leu Val Pro Ile Glu 
        435                 440                 445             


Met Glu Gly Leu Leu Ala Tyr Thr Pro Thr Lys Phe Gln His Leu Ser 
    450                 455                 460                 


Met Lys Ala Met Arg Asn Ile Ile Pro Phe Leu Glu Lys Gly Met Thr 
465                 470                 475                 480 


Tyr Asp Lys Ala Cys Glu Glu Ala Gly Tyr Asp Phe Lys Ala Asp Ser 
                485                 490                 495     


Lys Gly Thr Lys Gln Lys Leu Leu Thr Gly Glu Asn Val Asn Gln Thr 
            500                 505                 510         


Ile Asn Glu Ile Thr Asn Pro Val Val Lys Arg Ser Val Ser Gln Thr 
        515                 520                 525             


Val Lys Val Ile Asn Ala Ile Ile Arg Thr Tyr Gly Ser Pro Gln Ala 
    530                 535                 540                 


Ile Asn Ile Glu Leu Ala Arg Glu Met Ser Lys Thr Phe Glu Glu Arg 
545                 550                 555                 560 


Arg Lys Ile Lys Gly Asp Met Glu Lys Arg Gln Lys Asn Asn Glu Asp 
                565                 570                 575     


Val Lys Lys Gln Ile Gln Glu Leu Gly Lys Leu Ser Pro Thr Gly Gln 
            580                 585                 590         


Asp Ile Leu Lys Tyr Arg Leu Trp Gln Glu Gln Gln Gly Ile Cys Met 
        595                 600                 605             


Tyr Ser Gly Lys Thr Ile Pro Leu Glu Glu Leu Phe Lys Pro Gly Tyr 
    610                 615                 620                 


Asp Ile Asp His Ile Leu Pro Tyr Ser Ile Thr Phe Asp Asp Ser Phe 
625                 630                 635                 640 


Arg Asn Lys Val Leu Val Thr Ser Gln Glu Asn Arg Gln Lys Gly Asn 
                645                 650                 655     


Arg Thr Pro Tyr Glu Tyr Met Gly Asn Asp Glu Gln Arg Trp Asn Glu 
            660                 665                 670         


Phe Glu Thr Arg Val Lys Thr Thr Ile Arg Asp Tyr Lys Lys Gln Gln 
        675                 680                 685             


Lys Leu Leu Lys Lys His Phe Ser Glu Glu Glu Arg Ser Glu Phe Lys 
    690                 695                 700                 


Glu Arg Asn Leu Thr Asp Thr Lys Tyr Ile Thr Thr Val Ile Tyr Asn 
705                 710                 715                 720 


Met Ile Arg Gln Asn Leu Glu Met Ala Pro Leu Asn Arg Pro Glu Lys 
                725                 730                 735     


Lys Lys Gln Val Arg Ala Val Asn Gly Ala Ile Thr Ala Tyr Leu Arg 
            740                 745                 750         


Lys Arg Trp Gly Leu Pro Gln Lys Asn Arg Glu Thr Asp Thr His His 
        755                 760                 765             


Ala Met Asp Ala Val Val Ile Ala Cys Cys Thr Asp Gly Met Ile Gln 
    770                 775                 780                 


Lys Ile Ser Arg Tyr Thr Lys Val Arg Glu Arg Cys Tyr Ser Lys Gly 
785                 790                 795                 800 


Thr Glu Phe Val Asp Ala Glu Thr Gly Glu Ile Phe Arg Pro Glu Asp 
                805                 810                 815     


Tyr Ser Arg Ala Glu Trp Asp Glu Ile Phe Gly Val His Ile Pro Lys 
            820                 825                 830         


Pro Trp Glu Thr Phe Arg Ala Glu Leu Asp Val Arg Met Gly Asp Asp 
        835                 840                 845             


Pro Lys Gly Phe Leu Asp Thr His Ser Asp Val Ala Leu Glu Leu Asp 
    850                 855                 860                 


Tyr Pro Glu Tyr Ile Tyr Glu Asn Leu Arg Pro Ile Phe Val Ser Arg 
865                 870                 875                 880 


Met Pro Asn His Lys Val Thr Gly Ala Ala His Ala Asp Thr Ile Arg 
                885                 890                 895     


Ser Pro Arg His Phe Lys Asp Glu Gly Ile Val Leu Thr Lys Thr Ala 
            900                 905                 910         


Leu Thr Asp Leu Lys Leu Asp Lys Asp Gly Glu Ile Asp Gly Tyr Tyr 
        915                 920                 925             


Asn Pro Gln Ser Asp Leu Leu Leu Tyr Glu Ala Leu Lys Lys Gln Leu 
    930                 935                 940                 


Leu Leu Tyr Gly Asn Asp Ala Lys Lys Ala Phe Ala Gln Asp Phe His 
945                 950                 955                 960 


Lys Pro Lys Ala Asp Gly Thr Glu Gly Pro Val Val Arg Lys Val Lys 
                965                 970                 975     


Ile Gln Lys Lys Gln Thr Met Gly Val Phe Val Asp Ser Gly Asn Gly 
            980                 985                 990         


Ile Ala Glu Asn Gly Gly Met Val  Arg Ile Asp Val Phe  Arg Val Asn 
        995                 1000                 1005             


Gly Lys  Tyr Tyr Phe Val Pro  Val Tyr Thr Ala Asp  Val Val Lys 
    1010                 1015                 1020             


Lys Val  Leu Pro Asn Arg Ala  Ser Thr Ala His Lys  Pro Tyr Gly 
    1025                 1030                 1035             


Glu Trp  Lys Val Met Glu Asp  Lys Asp Phe Leu Phe  Ser Leu Tyr 
    1040                 1045                 1050             


Ser Arg  Asp Leu Ile His Ile  Lys Ser Lys Lys Asp  Ile Pro Ile 
    1055                 1060                 1065             


Lys Met  Val Asn Gly Gly Met  Glu Gly Ile Lys Glu  Thr Tyr Ala 
    1070                 1075                 1080             


Tyr Tyr  Ile Gly Ala Asp Ile  Ser Ala Ala Asn Ile  Gln Gly Ile 
    1085                 1090                 1095             


Ala His  Asp Ser Arg Tyr Lys  Phe Arg Gly Leu Gly  Ile Gln Ser 
    1100                 1105                 1110             


Leu Asp  Val Leu Glu Lys Cys  Gln Ile Asp Val Leu  Gly His Val 
    1115                 1120                 1125             


Ser Val  Val Arg Ser Glu Lys  Arg Met Gly Phe Ser  
    1130                 1135                 1140 


<210>  733
<211>  1140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  733

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Glu Lys Asn Ser Gly Lys Ile Val His Ala Ser Ser 
            20                  25                  30          


Arg Ile Phe Pro Ala Ala Thr Ala Asp Asn Asn Val Glu Arg Arg Lys 
        35                  40                  45              


Asn Arg Gln Ala Arg Arg Leu Asn Arg Arg Lys Lys His Arg Gly Val 
    50                  55                  60                  


Arg Phe Gln Asp Leu Phe Glu Asp Tyr Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Arg Leu Arg Val Asp Gly Leu 
                85                  90                  95      


Asn Gln Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Thr Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Cys Arg Leu Ile Asn Val Phe Ser Thr Ser Ala Tyr Lys Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Asp Phe Asn Asn Gln Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Lys Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Thr Thr His Lys Asp Ser Lys Gly Glu Tyr Val Thr Leu Asp Asn 
                245                 250                 255     


Ile Phe Gly Val Leu Ile Gly Lys Cys Thr Phe Tyr Pro Asn Glu Tyr 
            260                 265                 270         


Arg Ala Ser Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Glu 
    290                 295                 300                 


Glu Gln Lys Lys Thr Ile Ile Glu Tyr Ala Lys Ser Ala Lys Thr Leu 
305                 310                 315                 320 


Gly Ala Ser Thr Leu Leu Lys Tyr Ile Ala Lys Met Ile Asp Thr Ser 
                325                 330                 335     


Val Asp Gln Ile Arg Gly Tyr Arg Val Asp Val Asn Asn Lys Pro Glu 
            340                 345                 350         


Ile His Thr Phe Glu Val Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile 
        355                 360                 365             


Ser Val Gly Glu Leu Ser Arg Asn Val Leu Asp Glu Leu Ala His Ile 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Arg Glu Gly Ile Glu Glu Ala Ile Asn Thr 
385                 390                 395                 400 


Lys Leu Lys Asp Ser Phe Ser Gln Asp Gln Val Leu Glu Leu Val Gln 
                405                 410                 415     


Phe Arg Lys Asn Asn Ser Ser Leu Phe Ser Lys Gly Trp His Asn Phe 
            420                 425                 430         


Ser Leu Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser 
        435                 440                 445             


Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys 
    450                 455                 460                 


Glu Thr Ser Lys Arg Thr Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu 
465                 470                 475                 480 


Glu Ile Tyr Asn Pro Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys 
                485                 490                 495     


Ile Ile Asn Glu Ala Thr Lys Lys His Gly Ile Phe Asp Asn Ile Val 
            500                 505                 510         


Ile Glu Met Ala Arg Glu Asn Asn Glu Glu Asp Ala Lys Lys Asp Tyr 
        515                 520                 525             


Ile Lys Arg Gln Lys Ala Asn Gln Asp Glu Lys Tyr Ala Ala Met Glu 
    530                 535                 540                 


Lys Ala Ala Phe Gln Tyr Asn Gly Lys Lys Glu Leu Pro Asp Asn Ile 
545                 550                 555                 560 


Phe His Gly His Lys Glu Leu Ala Thr Lys Ile Arg Leu Trp His Gln 
                565                 570                 575     


Gln Gly Glu Lys Cys Leu Tyr Thr Gly Lys Ser Ile Pro Ile Ser Asp 
            580                 585                 590         


Leu Ile His Asn Gln Tyr Lys Tyr Glu Ile Asp His Ile Leu Pro Leu 
        595                 600                 605             


Ser Leu Ser Phe Asp Asp Ser Leu Ser Asn Lys Val Leu Val Leu Ala 
    610                 615                 620                 


Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp 
625                 630                 635                 640 


Ser Met Asp Asp Ala Trp Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys 
                645                 650                 655     


Glu Ser Lys Leu Leu Gly Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu 
            660                 665                 670         


Glu Asp Ile Ser Lys Ile Glu Val Lys Gln Lys Phe Ile Glu Arg Asn 
        675                 680                 685             


Leu Val Asp Thr Arg Tyr Ser Ser Arg Val Val Leu Asn Ala Leu Gln 
    690                 695                 700                 


Asp Phe Tyr Lys Asn His Asn Phe Asp Thr Thr Ile Ser Val Val Arg 
705                 710                 715                 720 


Gly Gln Phe Thr Ser Gln Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser 
                725                 730                 735     


Arg Glu Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala Ala 
            740                 745                 750         


Ser Ser Gln Leu Arg Leu Trp Lys Lys Gln Asn Asn Pro Leu Ile Ala 
        755                 760                 765             


Tyr Lys Glu Gly Gln Phe Val Asp Ser Gln Thr Gly Glu Ile Ile Ser 
    770                 775                 780                 


Leu Thr Asp Asp Glu Tyr Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp 
785                 790                 795                 800 


His Phe Val Asp Thr Leu Ser Ser Lys Thr Phe Glu Asp Ser Ile Leu 
                805                 810                 815     


Phe Ser Tyr Gln Val Asp Ser Lys Phe Asn Arg Lys Ile Ser Asp Ala 
            820                 825                 830         


Thr Ile Tyr Ala Thr Arg Lys Ala Lys Leu Asp Lys Glu Lys Lys Glu 
        835                 840                 845             


Tyr Thr Tyr Thr Leu Gly Lys Ile Lys Asp Ile Tyr Ser Leu Gly Thr 
    850                 855                 860                 


Lys Thr Pro Ser Lys Thr Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Asn 
865                 870                 875                 880 


Lys Asp Lys Ser Gln Phe Leu Met Phe Gln Lys Asp Arg Lys Thr Trp 
                885                 890                 895     


Asp Glu Val Ile Glu Lys Ile Met Glu Gln Tyr Arg Pro Phe Lys Glu 
            900                 905                 910         


Tyr Asp Lys Ala Gly Lys Leu Val Asp Phe Asn Pro Phe Glu Lys Tyr 
        915                 920                 925             


Arg Gln Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly Asn Gly 
    930                 935                 940                 


Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ile Leu Leu Gly Lys His 
945                 950                 955                 960 


Lys Asn Ile Thr Pro Glu Gly Ser Arg Asn Thr Val Ala Leu Leu Ser 
                965                 970                 975     


Leu Asn Pro Trp Arg Thr Asp Val Tyr Tyr Asn Met Glu Thr Lys Lys 
            980                 985                 990         


Tyr Glu Phe Leu Gly Leu Lys Tyr  Ala Asp Leu Pro Phe  Glu Glu Gly 
        995                 1000                 1005             


Gly Ala  Tyr Gly Ile Ser Thr  Glu Thr Tyr Asn Glu  Leu Arg Glu 
    1010                 1015                 1020             


Lys Glu  Gly Ile Gly Lys Asn  Ser Glu Phe Lys Phe  Thr Leu Tyr 
    1025                 1030                 1035             


Lys Asn  Asp Leu Ile Leu Ile  Lys Asp Thr Glu Thr  Asn Cys Gln 
    1040                 1045                 1050             


Gln Phe  Phe Arg Phe Trp Ser  Arg Thr Gly Lys Asp  Asn Pro Lys 
    1055                 1060                 1065             


Ser Phe  Glu Lys His Lys Ile  Glu Leu Lys Pro Tyr  Glu Lys Ala 
    1070                 1075                 1080             


Lys Phe  Glu Lys Gly Glu Glu  Leu Glu Val Leu Gly  Lys Val Pro 
    1085                 1090                 1095             


Pro Ser  Ser Asn Gln Phe Gln  Lys Asn Met Gln Ile  Glu Asn Leu 
    1100                 1105                 1110             


Ser Ile  Tyr Lys Val Lys Thr  Asp Val Leu Gly Asn  Lys His Phe 
    1115                 1120                 1125             


Ile Lys  Lys Glu Gly Asp Lys  Pro Lys Leu Lys Phe  
    1130                 1135                 1140 


<210>  734
<211>  1140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  734

Met Asn Gly Leu Val Leu Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Val Gly Ile Leu Lys Lys Asp Ile Gly Glu Ile Ile His Thr Asn Ser 
            20                  25                  30          


Arg Leu Phe Ser Ala Ala Thr Ala Asp Ser Asn Ile Glu Arg Arg Gly 
        35                  40                  45              


His Arg Gly Gly Lys Arg Leu Thr Arg Arg Lys Lys His Arg Ser Ile 
    50                  55                  60                  


Arg Leu His Asp Leu Phe Glu Asp Phe Gly Leu Leu Thr Asp Phe Ser 
65                  70                  75                  80  


Lys Val Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Gln Gly Leu 
                85                  90                  95      


Asp Asn Gln Leu Thr Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Ile 
            100                 105                 110         


Val Lys Arg Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Glu Asp Gly 
        115                 120                 125             


Gly Thr Val Ser Ser Asp Tyr Gly Lys Ala Val Glu Glu Asn Arg Lys 
    130                 135                 140                 


Leu Leu Ala Glu Gln Thr Pro Gly Gln Ile Gln Leu Asp Arg Phe Glu 
145                 150                 155                 160 


Lys Tyr Gly Gln Val Arg Gly Asp Phe Asn Val Val Glu Asn Gly Glu 
                165                 170                 175     


Lys Arg Arg Leu Ile Asn Val Phe Thr Thr Ser Ala Tyr Ser Lys Glu 
            180                 185                 190         


Ala Glu Arg Ile Leu Arg Lys Gln Gln Glu Phe Asn Lys Lys Ile Thr 
        195                 200                 205             


Asp Glu Phe Ile Glu Asp Tyr Leu Thr Ile Leu Thr Gly Lys Arg Lys 
    210                 215                 220                 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
225                 230                 235                 240 


Tyr Thr Thr Lys Lys Asp Pro Glu Gly Lys Tyr Ile Thr Leu Asp Asn 
                245                 250                 255     


Ile Phe Gly Ile Leu Ile Gly Lys Cys Thr Phe Tyr Pro Asp Glu Tyr 
            260                 265                 270         


Arg Ala Ser Lys Ala Ser Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn 
        275                 280                 285             


Asp Leu Asn Asn Leu Thr Val Pro Thr Glu Thr Lys Lys Leu Ser Glu 
    290                 295                 300                 


Glu Gln Lys Lys Thr Ile Ile Lys Tyr Ala Lys Thr Ala Lys Thr Leu 
305                 310                 315                 320 


Gly Ala Ser Thr Leu Leu Lys Tyr Ile Ala Lys Leu Ile Gly Ala Ser 
                325                 330                 335     


Val Asp Gln Ile His Gly Tyr Arg Ile Asp Pro Asn Lys Lys Pro Glu 
            340                 345                 350         


Met His Thr Phe Glu Thr Tyr Arg Lys Met Gln Ser Leu Glu Thr Ile 
        355                 360                 365             


Ser Val Glu Glu Leu Pro Arg Lys Val Leu Asp Glu Leu Ala His Ile 
    370                 375                 380                 


Leu Thr Leu Asn Thr Glu Arg Glu Gly Ile Glu Glu Ala Ile Asn Ala 
385                 390                 395                 400 


Thr Leu Lys Asp Thr Phe Ser Gln Asp Gln Val Leu Glu Leu Val Gln 
                405                 410                 415     


Phe Arg Lys Asn Asn Ser Ser Leu Phe Ser Lys Gly Trp His Ser Phe 
            420                 425                 430         


Ser Leu Lys Leu Met Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser 
        435                 440                 445             


Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Lys Gln Lys Ser Lys 
    450                 455                 460                 


Glu Thr Ser Lys Arg Thr Lys Tyr Ile Asp Glu Lys Glu Leu Thr Glu 
465                 470                 475                 480 


Glu Ile Tyr Asn Pro Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys 
                485                 490                 495     


Ile Ile Asn Glu Ala Thr Lys Lys Tyr Gly Ile Phe Asp Asn Ile Val 
            500                 505                 510         


Ile Glu Met Ala Arg Glu Asn Asn Glu Glu Asp Ala Lys Lys Glu Tyr 
        515                 520                 525             


Ile Lys Arg Gln Lys Ala Asn Leu Asp Glu Lys Asn Ala Ala Met Glu 
    530                 535                 540                 


Lys Ala Ala Phe Gln Tyr Asn Gly Lys Lys Glu Leu Pro Asp Asn Val 
545                 550                 555                 560 


Phe His Gly His Lys Glu Leu Ala Thr Lys Ile Arg Leu Trp His Gln 
                565                 570                 575     


Gln Gly Glu Lys Cys Leu Tyr Thr Gly Lys Asn Ile Pro Ile Ser Asp 
            580                 585                 590         


Leu Ile Gln Asn Gln Tyr Lys Tyr Glu Ile Asp His Ile Leu Pro Leu 
        595                 600                 605             


Ser Leu Ser Phe Asp Asp Ser Leu Ser Asn Lys Val Leu Val Leu Ala 
    610                 615                 620                 


Thr Ala Asn Gln Glu Lys Gly Gln Arg Thr Pro Phe Gln Ala Leu Asp 
625                 630                 635                 640 


Ser Met Asp Asp Ala Trp Ser Tyr Arg Glu Phe Lys Ser Tyr Val Lys 
                645                 650                 655     


Asp Ser Lys Leu Leu Gly Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu 
            660                 665                 670         


Glu Asp Ile Ser Lys Ile Glu Val Lys Gln Lys Phe Ile Glu Arg Asn 
        675                 680                 685             


Leu Val Asp Thr Arg Tyr Ser Ser Arg Val Val Leu Asn Ala Leu Gln 
    690                 695                 700                 


Asp Phe Tyr Lys Glu His Gln Phe Asp Thr Thr Ile Ser Val Val Arg 
705                 710                 715                 720 


Gly Gln Phe Thr Ser Gln Leu Arg Arg Lys Trp Gly Leu Glu Lys Ser 
                725                 730                 735     


Arg Glu Thr Tyr His His His Ala Val Asp Ala Leu Ile Ile Ala Ala 
            740                 745                 750         


Ser Ser Gln Leu Arg Leu Trp Lys Lys Gln Asn Asn Pro Leu Ile Ser 
        755                 760                 765             


Tyr Thr Glu Gly Gln Phe Val Asp Gln Val Thr Gly Glu Ile Ile Ser 
    770                 775                 780                 


Leu Ser Asp Asp Glu Tyr Lys Glu Leu Val Phe Lys Ala Pro Tyr Asp 
785                 790                 795                 800 


His Phe Val Asp Thr Leu Lys Ser Lys Lys Phe Glu Asp Ser Ile Leu 
                805                 810                 815     


Phe Ser Tyr Gln Val Asp Ser Lys Tyr Asn Arg Lys Ile Ser Asp Ala 
            820                 825                 830         


Thr Ile Tyr Ala Thr Arg Lys Ala Lys Leu Asp Lys Glu Asn Lys Glu 
        835                 840                 845             


Tyr Thr Tyr Thr Leu Gly Lys Ile Lys Asp Ile Tyr Ala Leu Gly Thr 
    850                 855                 860                 


Lys Ser Pro Ser Lys Thr Gly Phe Tyr Lys Phe Leu Asp Leu Tyr Asn 
865                 870                 875                 880 


Lys Asp Lys Ser Gln Phe Leu Met Phe Gln Lys Asp Arg Lys Thr Trp 
                885                 890                 895     


Asp Glu Val Ile Glu Lys Ile Ile Glu Gln Tyr Arg Pro Phe Lys Glu 
            900                 905                 910         


Tyr Asp Glu Asn Gly Lys Glu Val Asp Phe Asn Pro Phe Glu Lys Tyr 
        915                 920                 925             


Arg Ile Glu Asn Gly Pro Ile Arg Lys Tyr Ser Lys Lys Gly Asn Gly 
    930                 935                 940                 


Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Asn Leu Leu Gly Lys Phe 
945                 950                 955                 960 


Val Asp Ile Thr Pro Ser Glu Ser Lys Asn Pro Val Ala Leu Leu Ser 
                965                 970                 975     


Leu Asn Pro Trp Arg Thr Asp Val Tyr Tyr Asn Thr Glu Thr Ser Lys 
            980                 985                 990         


Tyr Glu Phe Leu Gly Leu Lys Tyr  Ala Asp Leu Cys Phe  Glu Lys Gly 
        995                 1000                 1005             


Gly Ala  Tyr Gly Ile Ser Glu  Val Lys Tyr Asn Lys  Ile Arg Glu 
    1010                 1015                 1020             


Lys Glu  Gly Ile Gly Lys Glu  Ser Glu Phe Lys Phe  Thr Leu Tyr 
    1025                 1030                 1035             


Lys Asn  Asp Leu Ile Leu Ile  Lys Asp Thr Glu Thr  Asn Cys Gln 
    1040                 1045                 1050             


Gln Ile  Phe Arg Phe Trp Ser  Arg Thr Gly Lys Asp  Asn Pro Lys 
    1055                 1060                 1065             


Ser Phe  Glu Lys His Lys Ile  Glu Leu Lys Pro Tyr  Glu Lys Ala 
    1070                 1075                 1080             


Arg Phe  Glu Lys Gly Glu Glu  Leu Glu Val Leu Gly  Lys Val Pro 
    1085                 1090                 1095             


Pro Ser  Ser Asn Gln Leu Gln  Lys Asn Met Gln Ile  Glu Asn Leu 
    1100                 1105                 1110             


Ser Ile  Tyr Lys Val Lys Thr  Asp Val Leu Gly Asn  Lys His Phe 
    1115                 1120                 1125             


Ile Lys  Lys Glu Gly Glu Glu  Pro Lys Leu Lys Phe  
    1130                 1135                 1140 


<210>  735
<211>  1140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  735

Met Glu Glu Asn Phe Gln Tyr Arg Ile Gly Leu Asp Ile Gly Ile Thr 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Leu Gln Asn Asn Ser Gln Asp Glu Pro Val 
            20                  25                  30          


Arg Ile Thr Asp Leu Gly Val Arg Ile Phe Asp Val Ala Glu Asn Pro 
        35                  40                  45              


Lys Asn Gly Asp Ala Leu Ala Ala Pro Arg Arg Asp Ala Arg Thr Thr 
    50                  55                  60                  


Arg Arg Arg Leu Arg Arg Arg Arg His Arg Leu Glu Arg Ile Lys Phe 
65                  70                  75                  80  


Leu Leu Gln Glu Asn Gly Leu Ile Glu Met Asp Ser Phe Met Glu Arg 
                85                  90                  95      


Tyr Tyr Lys Gly Asn Leu Pro Asp Val Tyr Gln Leu Arg Tyr Glu Gly 
            100                 105                 110         


Leu Asp Arg Lys Leu Lys Asp Glu Glu Leu Ala Gln Val Leu Ile His 
        115                 120                 125             


Ile Ala Lys His Arg Gly Phe Arg Ser Thr Arg Lys Ala Glu Thr Lys 
    130                 135                 140                 


Gly Lys Glu Gly Gly Ala Val Leu Lys Ala Thr Thr Glu Asn Gln Lys 
145                 150                 155                 160 


Ile Met Gln Glu Lys Gly Tyr Arg Thr Val Gly Glu Met Leu Tyr Leu 
                165                 170                 175     


Asp Glu Ala Phe His Thr Glu Cys Leu Trp Asn Glu Lys Gly Tyr Val 
            180                 185                 190         


Leu Thr Pro Arg Asn Arg Pro Asp Asp Tyr Lys His Thr Ile Leu Arg 
        195                 200                 205             


Ser Met Leu Val Glu Glu Val His Ala Ile Phe Ala Ala Gln Arg Ala 
    210                 215                 220                 


Leu Gly Asn Glu Lys Ala Thr Glu Glu Leu Glu Asn Ala Tyr Val Glu 
225                 230                 235                 240 


Ile Met Thr Ser Gln Arg Ser Phe Asp Met Gly Pro Gly Leu Gln Pro 
                245                 250                 255     


Asp Gly Lys Pro Ser Pro Tyr Ala Met Glu Gly Phe Gly Asp Arg Val 
            260                 265                 270         


Gly Lys Cys Thr Phe Glu Lys Asp Glu Tyr Arg Ala Pro Lys Ala Thr 
        275                 280                 285             


Tyr Thr Ala Glu Leu Phe Val Ala Leu Gln Lys Ile Asn His Thr Lys 
    290                 295                 300                 


Leu Ile Asp Glu Phe Gly Thr Gly Arg Phe Phe Thr Glu Glu Glu Arg 
305                 310                 315                 320 


Lys Thr Ile Ile Asp Leu Leu Leu Ser Gly Lys Glu Leu Lys Tyr Gly 
                325                 330                 335     


Thr Ile Arg Lys Lys Leu Asn Ile Asp Pro Ser Leu Lys Phe Asn Ser 
            340                 345                 350         


Leu Asn Tyr Ser Ala Lys Lys Glu Gly Glu Thr Glu Glu Glu Arg Val 
        355                 360                 365             


Arg Asp Thr Glu Lys Ala Lys Phe Ala Ser Met Phe Trp Thr Tyr Glu 
    370                 375                 380                 


Tyr Ser Lys Cys Leu Lys Asp Arg Asn Glu Glu Met Thr Val Gly Gln 
385                 390                 395                 400 


Lys Ala Asp Leu Leu Asp Arg Ile Gly Glu Ile Leu Thr Ala Tyr Lys 
                405                 410                 415     


Asn Asp Asp Ser Arg Ser Ser Arg Leu Glu Asp Leu Gly Leu Ser Arg 
            420                 425                 430         


Glu Glu Ile Asp Gly Leu Leu Glu Leu Ser Pro Ala Lys Tyr Gln Arg 
        435                 440                 445             


Val Ser Leu Lys Ala Met Arg Lys Met Gln Pro Tyr Leu Glu Asp Gly 
    450                 455                 460                 


Leu Ile Tyr Asp Lys Ala Cys Glu Ala Ala Gly Tyr Asp Phe Arg Ala 
465                 470                 475                 480 


Leu Asn Asp Gly Asn Lys Lys His Leu Leu Lys Gly Glu Glu Ile Asn 
                485                 490                 495     


Ala Ile Val Asn Asp Ile Thr Asn Pro Val Val Lys Arg Ser Val Ser 
            500                 505                 510         


Gln Thr Ile Lys Val Ile Asn Ala Ile Ile Gln Lys Tyr Gly Ser Pro 
        515                 520                 525             


Gln Ala Val Asn Ile Glu Leu Ala Arg Glu Met Ser Lys Asn Phe Gln 
    530                 535                 540                 


Asp Arg Thr Asn Leu Glu Lys Glu Met Lys Lys Arg Gln Gln Glu Asn 
545                 550                 555                 560 


Glu Arg Ala Lys Gln Gln Ile Ile Glu Leu Gly Lys Gln Asn Pro Thr 
                565                 570                 575     


Gly Gln Asp Ile Leu Lys Tyr Arg Leu Trp Asn Asp Gln Gly Gly Tyr 
            580                 585                 590         


Cys Leu Tyr Ser Gly Lys Lys Ile Pro Leu Glu Glu Leu Phe Gly Gly 
        595                 600                 605             


Gly Tyr Asp Ile Asp His Ile Leu Pro Tyr Ser Ile Thr Phe Asp Asp 
    610                 615                 620                 


Ser Tyr Arg Asn Lys Val Leu Val Thr Ala Gln Glu Asn Arg Gln Lys 
625                 630                 635                 640 


Gly Asn Arg Thr Pro Tyr Glu Tyr Phe Gly Ala Asp Glu Lys His Trp 
                645                 650                 655     


Glu Asp Tyr Glu Ala Arg Val Arg Leu Leu Val Arg Asp Tyr Lys Lys 
            660                 665                 670         


Gln Gln Lys Leu Leu Lys Lys Asn Phe Thr Glu Glu Glu Arg Lys Glu 
        675                 680                 685             


Phe Lys Glu Arg Asn Leu Asn Asp Thr Lys Tyr Ile Thr Arg Val Val 
    690                 695                 700                 


Tyr Asn Met Ile Arg Gln Asn Leu Glu Leu Glu Pro Phe Asn His Pro 
705                 710                 715                 720 


Glu Lys Lys Lys Gln Val Trp Ala Val Asn Gly Ala Val Thr Ser Tyr 
                725                 730                 735     


Leu Arg Lys Arg Trp Gly Leu Met Gln Lys Asp Arg Ser Thr Asp Arg 
            740                 745                 750         


His His Ala Met Asp Ala Val Val Ile Ala Cys Cys Thr Asp Gly Met 
        755                 760                 765             


Ile His Lys Ile Ser Arg Tyr Met Gln Gly Arg Glu Leu Ala Tyr Ser 
    770                 775                 780                 


Arg Asn Phe Lys Phe Pro Asp Glu Glu Thr Gly Glu Ile Leu Asn Arg 
785                 790                 795                 800 


Asp Asn Phe Thr Arg Glu Gln Trp Asp Glu Lys Phe Gly Val Lys Val 
                805                 810                 815     


Pro Leu Pro Trp Asn Ser Phe Arg Asp Glu Leu Asp Ile Arg Leu Leu 
            820                 825                 830         


Asn Glu Asp Pro Lys Asn Phe Leu Leu Thr His Ala Asp Val Gln Arg 
        835                 840                 845             


Glu Leu Asp Tyr Pro Gly Trp Met Tyr Gly Glu Glu Glu Ser Pro Ile 
    850                 855                 860                 


Glu Glu Gly Arg Tyr Ile Asn Tyr Ile Arg Pro Leu Phe Val Ser Arg 
865                 870                 875                 880 


Met Pro Asn His Lys Val Thr Gly Ser Ala His Asp Ala Thr Ile Arg 
                885                 890                 895     


Ser Ala Arg Asp Tyr Glu Thr Arg Gly Val Val Ile Thr Lys Val Pro 
            900                 905                 910         


Leu Thr Asp Leu Lys Leu Asn Lys Asp Asn Glu Ile Glu Gly Tyr Tyr 
        915                 920                 925             


Asp Lys Asp Ser Asp Arg Leu Leu Tyr Gln Ala Leu Val Arg Gln Leu 
    930                 935                 940                 


Leu Leu His Gly Asn Asp Gly Lys Lys Ala Phe Ala Glu Asp Phe His 
945                 950                 955                 960 


Lys Pro Lys Ala Asp Gly Thr Glu Gly Pro Val Val Arg Lys Val Lys 
                965                 970                 975     


Ile Glu Lys Lys Gln Thr Ser Gly Val Met Val Arg Gly Gly Thr Gly 
            980                 985                 990         


Ile Ala Ala Asn Gly Glu Met Val  Arg Ile Asp Val Phe  Arg Glu Asn 
        995                 1000                 1005             


Gly Lys  Tyr Tyr Phe Val Pro  Val Tyr Thr Ala Asp  Val Val Arg 
    1010                 1015                 1020             


Lys Val  Leu Pro Asn Arg Ala  Ala Met Asn Gly Lys  Ile Ala Ser 
    1025                 1030                 1035             


Asp Trp  Lys Thr Met Glu Asp  Asn Asn Phe Val Phe  Ser Leu Tyr 
    1040                 1045                 1050             


Ser Arg  Asp Leu Ile His Val  Lys Arg Lys Lys Ala  Ile Pro Thr 
    1055                 1060                 1065             


Ile Leu  Ser Ser Gly Ala Thr  Phe Glu Gln Glu Glu  Phe Tyr Ala 
    1070                 1075                 1080             


Tyr Phe  Thr Gly Ala Asp Ile  Ser Thr Val Ser Ile  Ala Gly Ile 
    1085                 1090                 1095             


Ser Asn  Asp Asn Ser Phe Lys  Phe Arg Gly Leu Gly  Ile Gln Gly 
    1100                 1105                 1110             


Leu Glu  Ile Phe Glu Lys Cys  Gln Val Asp Ile Leu  Gly Asn Ile 
    1115                 1120                 1125             


Ser Val  Val Arg His Glu Asn  Arg Gln Gly Phe His  
    1130                 1135                 1140 


<210>  736
<211>  1141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  736

Met Tyr Ser Ile Gly Leu Asp Ile Gly Ile Ala Ser Val Gly Tyr Ser 
1               5                   10                  15      


Val Ile Glu Thr Asn Ser Gly Arg Ile Val Glu Leu Gly Ala Arg Ile 
            20                  25                  30          


Phe Glu Ala Arg Asn Ser Asp Asn Asn Lys Asp Arg Arg Asp Phe Arg 
        35                  40                  45              


Gly Gly Arg Arg Leu Ile Arg Arg Arg Lys Thr Arg Leu Asp Asp Ala 
    50                  55                  60                  


Lys Arg Tyr Leu Asp Asp Gln Gly Phe Lys Asn Asp Lys Ser Leu Ile 
65                  70                  75                  80  


Asn Val Cys Pro Tyr Ser Leu Arg Val Lys Gly Leu Thr Glu Glu Leu 
                85                  90                  95      


Ser Lys Ser Glu Ile Arg Lys Val Ile Leu His Ile Leu Lys Lys Arg 
            100                 105                 110         


Gly Val Ser Tyr Leu Ser Ser Glu Asp Leu Asp Thr Ser Ser Val Asp 
        115                 120                 125             


Asn Lys Asp Ser Phe Lys Gln Ala Val Thr Lys Asn Leu Arg Gln Leu 
    130                 135                 140                 


Asp Asp Lys Thr Pro Gly Gln Ile Gln Tyr Glu Arg Leu Gln Glu Ser 
145                 150                 155                 160 


Gly Arg Val Arg Thr Gly Ile Asn Gln Asn Gly Gln Tyr Gln Leu Asn 
                165                 170                 175     


Val Phe Asp Val Ala Ser Tyr Ala Lys Glu Leu Lys Leu Ile Leu Glu 
            180                 185                 190         


Thr Gln Gln Gln Tyr His Gln Glu Ile Asp Asp His Phe Leu Asp Phe 
        195                 200                 205             


Phe Thr Lys Asp Gly Tyr Arg Glu Asp Ala Gly Leu Ile Tyr Arg Lys 
    210                 215                 220                 


Arg Pro Tyr Tyr Asp Gly Pro Gly Asn Ala Ala Gln Asn Ser Ser Tyr 
225                 230                 235                 240 


Gly Arg Trp Ala Asn Tyr Gln Val Asp Gly Lys Pro Ala Glu Asn Ile 
                245                 250                 255     


Phe Asp Gln Leu Ile Gly Lys Asp Phe Thr Gly Lys Ile Arg Ala Ser 
            260                 265                 270         


Asn Leu Gly Pro Ser Ala Gln Lys Phe Asn Leu Leu Asn Asp Leu Asn 
        275                 280                 285             


Asn Leu Arg Ile Thr Thr Arg Glu Glu Glu Lys Leu Thr Phe Glu Glu 
    290                 295                 300                 


Lys Gln Glu Ile Leu Glu Val Leu Glu Asn Asp Arg Pro Lys Thr Phe 
305                 310                 315                 320 


Gly Pro Arg Asn Ile Ala Lys Met Leu Lys Val Asp Met Asp Asp Ile 
                325                 330                 335     


Lys Gly Trp Arg Leu Asn Ser Ser Asp Lys Pro Glu Ile His Thr Met 
            340                 345                 350         


Lys Glu Tyr Asn Asp Met Arg Leu Val Leu Glu Ala Gly Gly Phe Asp 
        355                 360                 365             


Ile Lys Ala Ile Asp Leu Asp His Ile Glu Glu Ile Ala Ser Leu Ile 
    370                 375                 380                 


Thr Leu Asn Thr Glu Arg Glu Ala Phe Glu Leu Ser Leu Ser Asn Glu 
385                 390                 395                 400 


Ile Asp Asn Asn Leu Lys Gln Phe Leu Val Glu Asn Phe Asn Ala Leu 
                405                 410                 415     


Arg Lys Cys Lys Pro Asn Asn Ser Trp His Ser Phe Ser Lys Glu Asn 
            420                 425                 430         


Leu Gln Arg Leu Ile Thr Glu Leu Glu His Thr Ser Asp Glu Gln Asn 
        435                 440                 445             


Thr Ile Leu Glu Arg Leu Gly Leu Lys Phe Asp Leu Arg Ser Lys Tyr 
    450                 455                 460                 


Ser Asp Arg Asp Thr Leu Pro Ile His Glu Val Leu Glu Glu Ile Tyr 
465                 470                 475                 480 


Asn Pro Thr Val Ser Lys Ser Val Arg Gln Thr Phe Arg Val Leu Asn 
                485                 490                 495     


Ala Leu Val Lys Lys Tyr Gly Lys Glu Asn Ile Asp Tyr Val Thr Ile 
            500                 505                 510         


Glu Met Pro Arg Asp Lys Asn Glu Asp Asp Gln Lys Lys Thr Ile Arg 
        515                 520                 525             


Glu Ile Gln Lys Val Asn Lys Asn Arg Lys Asp Lys Ser Lys Glu Tyr 
    530                 535                 540                 


Phe Leu Lys Lys Ser Gly Trp Ser Glu Glu Lys Phe Glu Glu Lys Leu 
545                 550                 555                 560 


Arg Thr Ser Arg Arg Phe Ala Ser Lys Leu Tyr Tyr Phe Tyr Glu Gln 
                565                 570                 575     


Asp Gly Leu Cys Ala Tyr Thr Gly Gln Gln Ile Gln Pro Glu Asp Leu 
            580                 585                 590         


Leu Thr Glu Arg Val Glu Val Asp His Ile Ile Pro Leu Ser Val Ser 
        595                 600                 605             


Leu Asp Asp Ser Ile Asn Asn Lys Val Leu Val Thr Ser Glu Ala Asn 
    610                 615                 620                 


Gln Lys Lys Gly Gln Arg Ser Pro Tyr Asp Ala Phe Met Ser Gly Asp 
625                 630                 635                 640 


Phe Ser Lys Tyr Arg Thr Tyr Asp Glu Tyr Lys Ala Trp Val Leu Ser 
                645                 650                 655     


Asn Lys Tyr Lys Lys Tyr Lys Lys Ala Leu Leu Leu Glu Glu Arg Asn 
            660                 665                 670         


Ile Phe Asp Pro Glu Leu Ala Lys Lys Phe Arg Gln Arg Asn Leu Asn 
        675                 680                 685             


Asp Thr Arg Tyr Ala Ser Arg Ile Val Leu Asn Ala Val Gln Ser Phe 
    690                 695                 700                 


Phe Tyr Lys Thr Asp Thr Lys Thr Arg Val Val Asn Gly Ala Phe Thr 
705                 710                 715                 720 


His Thr Leu Arg Lys Lys Trp Asn Leu Ala Asp Lys Asp Arg Glu Thr 
                725                 730                 735     


His His His His Ala Ile Asp Ala Thr Leu Val Ala Val Thr Pro Phe 
            740                 745                 750         


Val Lys Ala Glu Arg Tyr Glu Tyr Tyr Lys Asp Ser Glu Gly Lys Ala 
        755                 760                 765             


Ser Met Val Asp Thr Val Thr Gly Thr Ile Ile Pro Tyr Ser Glu Tyr 
    770                 775                 780                 


Lys Lys Lys Asn Leu Tyr Asp Arg Ser Asn Tyr Leu Leu Met Phe Gly 
785                 790                 795                 800 


Asn Glu Ser Thr Ile Asn Gln Glu Lys Glu Asp Lys Ser Ala Glu Phe 
                805                 810                 815     


Ile Arg Asn Leu Ile Pro Ala Lys Ile Tyr Pro Glu Ile Lys Phe Ser 
            820                 825                 830         


His Gln Val Asp Arg Lys Ser Asn Arg Arg Leu Ser Asp Ala Thr Ile 
        835                 840                 845             


Tyr Ser Thr Arg Ser Tyr Thr Val Glu Lys Gly Arg Gly Lys Asn Lys 
    850                 855                 860                 


Arg Glu Val Ile Glu Arg Asp Ile Ile Gly Lys Ile Lys Asp Ile Tyr 
865                 870                 875                 880 


Thr Ala Asp Gly Tyr Ala Glu Tyr Val Lys Tyr Lys Asp Ser Leu Leu 
                885                 890                 895     


Val Lys Glu Ile Asp Glu Lys Thr Phe Glu Lys Leu Glu Ala Ile Ile 
            900                 905                 910         


Glu Glu Phe Ser Asp Thr Val Glu Val Thr Asp Gln Gln Gly Lys Val 
        915                 920                 925             


Lys Lys Val Ser Lys Ser Pro Phe Leu Gln Tyr Cys Glu Lys Tyr Asp 
    930                 935                 940                 


Val Pro Gly Val Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Val Ile 
945                 950                 955                 960 


Arg Ser Leu Lys Tyr Arg Tyr Thr Lys Leu Arg Ser His Ile Asp Ile 
                965                 970                 975     


Ser Pro Lys Asn Ser Lys Asn Thr Val Ala Leu Leu Thr Leu Ala Pro 
            980                 985                 990         


Trp Arg Thr Asp Val Tyr Tyr Ser  Gln Glu Thr Ala Ser  Tyr His Leu 
        995                 1000                 1005             


Leu Gly  Leu Lys Tyr Ile Asp  Leu Ser Phe Arg Asp  Gly Gly Tyr 
    1010                 1015                 1020             


Gly Val  Ser Arg Lys Arg Tyr  Glu Glu Ile Lys Lys  Asn Glu Arg 
    1025                 1030                 1035             


Ile Pro  Glu Asp Gly Glu Phe  Lys Phe Ser Leu Tyr  Lys Lys Asp 
    1040                 1045                 1050             


Tyr Ile  Glu Ile Glu Phe Asp  Lys Lys Ile Leu Pro  Gly Leu Tyr 
    1055                 1060                 1065             


Gln Ser  Arg Asp Val Ser Ala  Arg Asn Lys Phe Ser  Met Lys Pro 
    1070                 1075                 1080             


Ile Asp  Lys Ala Gln Trp Thr  Ser Lys Glu Asp Val  Leu Met Phe 
    1085                 1090                 1095             


Gly Asn  Val Lys Ala Ser Gly  Val Phe Glu Lys Thr  Leu Lys Lys 
    1100                 1105                 1110             


Asn Tyr  Val Leu Arg Lys Tyr  His Val Asp Val Leu  Gly Asn Lys 
    1115                 1120                 1125             


Phe Glu  Val Lys Asp Glu Ile  Leu Lys Asp Ile Leu  Glu 
    1130                 1135                 1140     


<210>  737
<211>  1142
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  737

Met Tyr Ser Val Gly Leu Asp Ile Gly Ile Ala Ser Cys Gly Tyr Ser 
1               5                   10                  15      


Ile Ile Asn Asp Val Asn Gly Asn Ile Val Glu Leu Gly Thr Leu Leu 
            20                  25                  30          


Phe Lys Ser Arg Asn Ser Asp Asn Asn Glu Glu Arg Arg Thr Asn Arg 
        35                  40                  45              


Gly Ser Arg Arg Leu Ile Asn Arg Arg Arg Thr Arg Leu Asp Asp Gly 
    50                  55                  60                  


Lys Lys Leu Leu Ala Glu Lys Gly Phe Lys Gln Asp Ser Lys Leu Lys 
65                  70                  75                  80  


His Val Cys Pro Tyr Glu Leu Arg Val Lys Gly Leu His Glu Lys Leu 
                85                  90                  95      


Thr Gln Gly Glu Ile Tyr Lys Val Val Leu His Ile Leu Lys Lys Arg 
            100                 105                 110         


Gly Ile Ser Tyr Leu Asp Glu Ser Asp Glu Asn Ser Ser Gly Ile Asp 
        115                 120                 125             


Gly Ala Ser Tyr Lys Asp Gln Val Asn Leu Asn Leu Asn Leu Gln Lys 
    130                 135                 140                 


Glu Leu Thr Pro Gly Gln Ile Gln Leu Lys Arg Leu Lys Glu Asn Gly 
145                 150                 155                 160 


Arg Ile Lys Ser Gly Val Thr Phe Glu Asn Gly Tyr Gln Leu Asn Val 
                165                 170                 175     


Phe Ser Val Ser Ala Tyr Ala Asn Glu Leu Asn Lys Ile Leu Thr Lys 
            180                 185                 190         


Gln Ser Glu Tyr Tyr Ser Glu Ile Asp Gln Ile Trp Ile Asp Arg Phe 
        195                 200                 205             


Thr Lys Asn Gly Tyr Arg Lys Glu Ala Gly Leu Val Tyr Arg Lys Arg 
    210                 215                 220                 


Pro Tyr Tyr Asp Gly Pro Gly Asn Glu Lys Asn Asn Ser Ile Tyr Gly 
225                 230                 235                 240 


Arg Trp Ala Asn Phe Lys Glu Asp Gly His Pro Lys Glu Asn Ile Phe 
                245                 250                 255     


Asp Gln Leu Ile Gly Leu Asp Ser Val Asp Ser Thr Gln Val Arg Ala 
            260                 265                 270         


Ser Ala Ser Thr Leu Thr Ala Gln Lys Tyr Asn Leu Leu Asn Asp Leu 
        275                 280                 285             


Asn Asn Leu Thr Ile Ile Glu Asp Asp Arg Lys Phe Thr Thr Asp Glu 
    290                 295                 300                 


Lys Val Ser Ile Ile Asn Tyr Leu Met Thr Gln Asp Ile Thr Arg Phe 
305                 310                 315                 320 


Gly Pro Lys Glu Leu Ala Lys Phe Tyr Gln Leu Glu Val Asp Gln Ile 
                325                 330                 335     


Arg Gly Trp Arg Ile Asp Asn Lys Gly Asn Pro Glu Ile His Ser Leu 
            340                 345                 350         


Lys Val Tyr Arg Asp Trp Lys Lys Ile Phe Asn Thr Ser Ser Ile Asp 
        355                 360                 365             


Leu Thr Ser Ile Gln Thr Trp Val Val Asp Glu Leu Ala Asn Val Ile 
    370                 375                 380                 


Thr Leu Asn Thr Glu Ile Pro Ser Thr Leu Ala Thr Leu Lys Ile Lys 
385                 390                 395                 400 


Ile Pro Asp Ile Asp Gln Gln Ile Leu Asn Phe Val Glu Asn Asn Phe 
                405                 410                 415     


Tyr Glu Leu Lys Lys Lys Thr Ser Lys Asn Ser Trp His Ser Phe Ser 
            420                 425                 430         


Leu Lys Thr Leu Asn Ile Leu Ile Pro Glu Leu Leu Asn Thr Ser Glu 
        435                 440                 445             


Glu Gln Asn Thr Ile Leu Glu Arg Leu Asn Leu Lys Phe Asp Leu Lys 
    450                 455                 460                 


Asn Lys Tyr Ala Glu Lys Asn Gln Ile Pro Val Lys Glu Leu Leu Glu 
465                 470                 475                 480 


Gln Ile Tyr Asn Pro Thr Val Thr Lys Ser Val His Gln Ser Ile Lys 
                485                 490                 495     


Leu Phe Asn Glu Ile Ile Glu Lys Tyr Gly Lys Asn Asn Ile Ser Tyr 
            500                 505                 510         


Val Thr Ile Glu Met Pro Arg Asp Lys Asn Asp Gln Asp Gln Lys Asp 
        515                 520                 525             


Thr Ile Lys Arg Ile Asn Lys Leu Asn Ser Glu Arg Lys Asn Asp Ser 
    530                 535                 540                 


Glu Lys Tyr Phe Leu Glu Arg Ser Gly Trp Asp Thr Ile Arg Phe Glu 
545                 550                 555                 560 


Ser Ala Leu Lys Lys Pro Arg Phe Ala Glu Lys Leu Leu Phe Tyr Tyr 
                565                 570                 575     


Glu Gln Lys Gly Lys Cys Ala Tyr Ser Gly Gln Asp Ile Leu Ala Glu 
            580                 585                 590         


Ser Leu Leu Asn Glu Ser Cys Glu Val Asp His Ile Ile Pro Leu Ser 
        595                 600                 605             


Ile Ser Phe Asp Asp Ser Ile His Asn Lys Val Leu Val Thr Ala Ser 
    610                 615                 620                 


Ser Asn Gln Glu Lys Gly Gln Arg Thr Pro Tyr Met Ala Phe Ile Asp 
625                 630                 635                 640 


Gly Ala Lys Leu Gly Gln Ser Trp Asp Asp Phe Val Ala Trp Val Asn 
                645                 650                 655     


Ser Cys Gly Phe His Arg Lys Lys Val Gln Asn Leu Leu Tyr Glu Glu 
            660                 665                 670         


Asn Ile Phe Asn Pro Asp Val Gln Arg Gly Phe Val Ser Arg Asn Leu 
        675                 680                 685             


Asn Asp Thr Arg Tyr Ala Ser Arg Ile Val Leu Asn Ala Ile Gln Ser 
    690                 695                 700                 


Phe Phe Tyr Lys Gln Lys Asp Leu Thr Lys Val Lys Val Val Asn Gly 
705                 710                 715                 720 


Ser Phe Thr His Thr Leu Arg Lys Lys Trp Gly Ser Ser Leu Asp Lys 
                725                 730                 735     


Asn Arg Asp Thr Tyr His His His Ala Val Asp Ala Thr Leu Cys Ala 
            740                 745                 750         


Val Thr Pro Phe Ile Asn Ile Val Lys Tyr Glu Phe Phe Glu Glu Asp 
        755                 760                 765             


Gly Ile Lys Tyr Met Val Asp Leu Thr Thr Gly Glu Lys Val Ala Tyr 
    770                 775                 780                 


Ser Asp Tyr Lys Lys Met Lys Phe Tyr Asp Lys Arg Ala Tyr Val Pro 
785                 790                 795                 800 


Ile Trp Asp Gly Phe Ile Lys Gln Leu Thr Pro Ala Asn Leu Tyr Pro 
                805                 810                 815     


Lys Ile Lys Phe Ser His Gln Val Asp Ser Lys Trp Asn Arg Lys Ile 
            820                 825                 830         


Ser Asp Ala Thr Ile Tyr Ser Thr Arg Lys Ile Val Glu Thr Lys Ile 
        835                 840                 845             


Asn Lys Lys Gly Val Ala Lys Glu Thr Val Lys Asp Ile Val Ile Asp 
    850                 855                 860                 


Lys Ile Lys Asp Ile Tyr Thr Pro Glu Gly Trp Arg Lys Phe Lys Glu 
865                 870                 875                 880 


Lys Gln Asp Lys Leu Leu Met Lys Asn Phe Asp Glu Lys Thr Tyr Asn 
                885                 890                 895     


Lys Leu Ile Lys Ile Ala Lys Glu Tyr Pro Glu Lys Lys Glu Val Gln 
            900                 905                 910         


Thr Ser Asn Gly Lys Val Ser Leu Val Glu Val Ser Pro Phe Lys Met 
        915                 920                 925             


Tyr Cys Glu Glu Gln Asn Val Pro Ala Ile Arg Lys Tyr Ser Lys Lys 
    930                 935                 940                 


Gly Asn Gly Pro Tyr Ile Arg Ser Leu Lys Phe Tyr Asp Lys Glu Leu 
945                 950                 955                 960 


Gly Lys His Ile Asn Ile Thr Lys Asp Ser Tyr Gly Asn Asp Ile Glu 
                965                 970                 975     


Arg Thr Ile Arg Asn Lys Lys Ile Ile Leu Lys Asn Gln Asn Ser Trp 
            980                 985                 990         


Arg Thr Asp Val Tyr Tyr Asn Ser  Val Asn Asn Cys Tyr  Glu Leu Leu 
        995                 1000                 1005             


Gly Ile  Lys Tyr Cys His Leu  Lys Phe Tyr Arg Gly  Lys Tyr Gly 
    1010                 1015                 1020             


Ile Pro  Lys Ser Ile Tyr Asp  Glu Leu Lys Lys Glu  Asp Glu Ile 
    1025                 1030                 1035             


Ser Ser  Glu Ser Val Phe Lys  Phe Ser Leu Tyr Arg  His Asp Arg 
    1040                 1045                 1050             


Val Arg  Phe Glu Lys Asp Gly  Glu Tyr Ile Glu Ala  Leu Phe Gly 
    1055                 1060                 1065             


Ser Lys  Asn Glu Ser Asn Lys  Asn Tyr Leu Glu Leu  Ser Pro Ile 
    1070                 1075                 1080             


Asp Lys  Asn Lys Trp Leu Ser  Lys Glu Asn Val Lys  Phe Phe Gly 
    1085                 1090                 1095             


Glu Val  Ala Ala Ser Gly Arg  Phe Ile Lys Gly Leu  Lys Pro Gly 
    1100                 1105                 1110             


Ile Lys  Val Ile Lys Gln Ser  Thr Asp Tyr Met Gly  His Val Tyr 
    1115                 1120                 1125             


Ala Ile  His Gln Glu Lys Leu  Lys Phe Val Leu Ser  Asp Ala 
    1130                 1135                 1140         


<210>  738
<211>  1144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  738

Met Thr Ser Lys Gln Glu Lys His Gly Ala Leu Thr Phe Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Val Leu Gly Glu Ser Arg Ile 
            20                  25                  30          


Val Asp Leu Gly Val Arg Cys Phe Asp Lys Ala Glu Thr Ala Lys Glu 
        35                  40                  45              


Gly Glu Ser Leu Asn Leu Thr Arg Arg Met Ala Arg Leu Leu Arg Arg 
    50                  55                  60                  


Arg Leu Arg Arg Arg Ala Trp Arg Leu Thr Lys Leu Ala Arg Leu Leu 
65                  70                  75                  80  


Lys Arg Glu Gly Leu Ile Ala Asp Ala Asn Leu Phe Lys His Lys Ile 
                85                  90                  95      


Glu Lys Asn Thr Pro Val Pro Lys Ser Ser Trp Gln Leu Arg Val Asp 
            100                 105                 110         


Gly Leu Asp Arg Gln Leu Asn Ala Glu Glu Trp Ala Arg Val Ile Tyr 
        115                 120                 125             


His Leu Cys Lys His Arg Gly Phe His Trp Ile Ser Arg Ala Glu Glu 
    130                 135                 140                 


Lys Ala Ala Glu Gly Asp Ser Lys Gly Glu Gly Gly Lys Val Lys Gln 
145                 150                 155                 160 


Gly Leu Ala Gly Thr Lys Lys Leu Met Gln Glu Lys Ser Tyr Arg Thr 
                165                 170                 175     


Ala Ala Glu Met Val Leu Ala Glu Phe Pro Asp Ala Gln Arg Asn Lys 
            180                 185                 190         


Gln Gly Asp Tyr Ser Lys Ala Leu Ser Arg Ile Leu Leu Gly Asp Glu 
        195                 200                 205             


Leu Lys Ala Leu Phe Glu His Gln Arg Lys Leu Gly Asn Pro Val Ala 
    210                 215                 220                 


Thr Glu Lys Leu Glu Ala Ala Ile Leu Gly Asn Gly Asp Lys Lys Cys 
225                 230                 235                 240 


Gly Leu Phe Trp Ala Gln Asn Pro Ala Leu Ala Gly Asn Lys Leu Leu 
                245                 250                 255     


Glu Met Leu Gly Arg Cys Thr Phe Glu Lys Thr Gly Gly Asp Asp Gly 
            260                 265                 270         


Lys Gly Glu Tyr Arg Ala Pro Lys Ala Ser Phe Ser Ala Glu Arg His 
        275                 280                 285             


Val Trp Leu Thr His Leu Asn Asn Leu Arg Ile Val Val Asp Gly Thr 
    290                 295                 300                 


Thr Arg Pro Leu Asn Glu Ala Glu Arg Arg Val Ala Leu Pro Met Pro 
305                 310                 315                 320 


Tyr Gln Gln Ala Gly Asp Leu Ser Tyr Lys Gln Leu Gly Gly Ala Leu 
                325                 330                 335     


Ile Lys Glu Gly Phe Leu Gln Lys Asp Gly Phe Lys Phe Ala Gly Leu 
            340                 345                 350         


Pro Tyr Pro Thr Lys Gln Ala Ser Glu Glu Lys Ala Lys Asp Pro Glu 
        355                 360                 365             


Thr Ser Val Leu Cys Lys Leu Pro Ala Trp His Thr Leu Arg Lys Thr 
    370                 375                 380                 


Leu Glu Lys His Gly Leu Glu Ser Glu Trp Gln Lys Leu Ser Thr Asp 
385                 390                 395                 400 


Ala Leu Asp Gly Lys Pro Gln Leu Leu Asp Asn Ile Ala Trp Val Leu 
                405                 410                 415     


Ser Val Tyr Lys Asp Asp Asp Glu Val Lys Thr Glu Leu Gly Lys Leu 
            420                 425                 430         


Asn Leu Pro Asn Thr Glu Lys Met Ile Asp Ala Leu Leu Asp Ile Arg 
        435                 440                 445             


Phe Asp Lys Phe Ser Asn Leu Ser Leu Lys Ala Leu Ser Lys Ile Val 
    450                 455                 460                 


Pro Phe Met Glu Gln Gly Gln Arg Tyr Asp Glu Ala Val Ala Asn Ile 
465                 470                 475                 480 


Pro Glu Tyr Gly His His Ser Gln Leu His Lys Val Gly Ala Gly Glu 
                485                 490                 495     


His Lys Tyr Leu Pro Pro Phe Tyr Lys Glu Arg Asp Lys Thr Gly Arg 
            500                 505                 510         


Met Val Phe Asn Asp Asp Met Asp Ile Pro Arg Asn Pro Val Val Leu 
        515                 520                 525             


Arg Ala Leu Asn Gln Ala Arg Lys Val Val Asn Ala Leu Ile Lys Glu 
    530                 535                 540                 


Tyr Gly Ser Pro His Glu Val His Ile Glu Met Ala Arg Asp Leu Ser 
545                 550                 555                 560 


Arg Pro Leu Asp Glu Arg Arg Asp Ile Glu Lys Leu Gln Lys Glu Phe 
                565                 570                 575     


Gly Glu Arg Asn Lys Lys Asp Lys Glu Leu Phe Ala Gln Asp Phe Asn 
            580                 585                 590         


Ile Val Gly Thr Val Lys Gly Lys Gln Phe Glu Lys Tyr Gln Leu Tyr 
        595                 600                 605             


Arg Glu Gln Gln Gly Lys Cys Ile Tyr Pro Ser Met Asp Glu Lys Gln 
    610                 615                 620                 


Gly Ile Cys Cys Tyr Ser Leu Lys Pro Leu Asp Leu His Arg Val Leu 
625                 630                 635                 640 


Tyr Glu Glu Gly Tyr Ala Glu Ile Asp His Ala Leu Pro Tyr Ser Arg 
                645                 650                 655     


Ser Phe Asp Asp Ser Lys Asn Asn Arg Val Leu Val Leu Ala Lys Glu 
            660                 665                 670         


Asn Arg Asp Lys Gly Asn Arg Thr Pro Phe Glu Tyr Leu Gly Asp Lys 
        675                 680                 685             


Asp Asn Ser Glu Pro Trp Arg Gln Phe Val Ala Phe Val Glu Gly Asn 
    690                 695                 700                 


Lys Ala Tyr Arg Leu Ala Lys Arg Ser Arg Leu Leu Arg Lys Asp Phe 
705                 710                 715                 720 


Gly Asp Lys Glu Ala Lys Glu Phe Arg Glu Arg Asn Leu Asn Asp Thr 
                725                 730                 735     


Arg Tyr Ile Cys Lys Phe Phe Lys Asn Tyr Val Glu Gln Tyr Leu Gln 
            740                 745                 750         


Leu His Glu Asp Ser Glu Ala Lys Arg Cys Val Val Leu Ser Gly Gln 
        755                 760                 765             


Met Thr Ser Phe Leu Arg Ala Arg Trp Gly Leu Val Lys Val Arg Gly 
    770                 775                 780                 


Asp Ser Asp Arg His His Ala Leu Asp Ala Ala Val Val Ala Ala Cys 
785                 790                 795                 800 


Ser His Gly Met Val Lys Arg Leu Ser Asp Tyr Ser Arg Arg Lys Glu 
                805                 810                 815     


Leu Glu Gln Val Arg Glu Gly Phe Val Asp Val Asp Thr Gly Glu Ile 
            820                 825                 830         


Val Asn Pro Ala Met Phe Gln Gln Val Gln Glu His Phe Pro Asn Pro 
        835                 840                 845             


Trp Pro His Phe Arg Asp Glu Val Lys Leu Arg Leu Ser Ile Asp Asp 
    850                 855                 860                 


Pro Thr Leu Leu Arg Ser Glu Met Glu Lys Phe Gly Thr Tyr Thr Pro 
865                 870                 875                 880 


Glu Ser Leu Glu Ala Leu Arg Pro Leu Phe Val Ser Arg Ala Pro Gln 
                885                 890                 895     


Arg Arg Asn Ser Gly Ala Ala His Lys Asp Thr Ile Tyr Ala Gln Pro 
            900                 905                 910         


Lys Arg Leu Val Ala Gln Gly Gly Val Thr Gln Lys Val Ala Leu Ser 
        915                 920                 925             


Ser Leu Thr Leu Lys Asp Leu Asp Lys Leu Ile Asp Pro His Arg Asn 
    930                 935                 940                 


Glu Arg Leu Tyr Ala Ala Ile Arg Ala Arg Leu Glu Gln His Gly Gly 
945                 950                 955                 960 


Lys Gly Asp Lys Ala Phe Pro Ala Ser Asn Pro Leu Arg Lys Pro Asp 
                965                 970                 975     


Ser Glu Gly Asn Pro Thr Gly Pro Ile Val Arg Gly Val Thr Met Val 
            980                 985                 990         


Ile Asp Lys Leu Ser Gly Ile Pro  Ile Arg Gly Gly Ile  Ala Lys Asn 
        995                 1000                 1005             


Asp Ser  Met Val Arg Val Asp  Val Phe Thr Lys Ala  Asn Lys Phe 
    1010                 1015                 1020             


His Leu  Val Pro Val Tyr Val  His His Ser Val Leu  Gly Leu Pro 
    1025                 1030                 1035             


Asn Gln  Ala Ile Val Gly Gly  Lys Glu Glu Ser Glu  Trp Thr Gln 
    1040                 1045                 1050             


Ile Asp  Asp Ser Phe Asp Trp  His Phe Ser Ile Tyr  Pro Asn Asp 
    1055                 1060                 1065             


Leu Ile  Arg Val Thr Leu Lys  Gln Glu Gly Arg Phe  Gly Tyr Tyr 
    1070                 1075                 1080             


Ala Gly  Cys Asp Arg Ser Thr  Ala Ala Ile His Leu  Trp Ala His 
    1085                 1090                 1095             


Asp Arg  Asn Gln Gln Val Gly  Lys Asp Gly Leu Ile  Arg Ser Ile 
    1100                 1105                 1110             


Gly Val  Lys Thr Ala Leu Ser  Ile Glu Lys Phe Asn  Val Asp Val 
    1115                 1120                 1125             


Leu Gly  Asn Arg Tyr Pro Ala  Pro Pro Glu Lys Arg  Arg Asp Leu 
    1130                 1135                 1140             


Ala 
    


<210>  739
<211>  1144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  739

Met Thr Ser Lys Gln Glu Asn Leu Gly Ala Leu Thr Phe Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Val Leu Gly Glu Asn Arg Ile 
            20                  25                  30          


Ile Asp Leu Gly Val Arg Cys Phe Asp Lys Ala Glu Thr Ala Lys Glu 
        35                  40                  45              


Gly Glu Ser Leu Asn Leu Thr Arg Arg Met Ala Arg Leu Leu Arg Arg 
    50                  55                  60                  


Arg Leu Arg Arg Arg Ala Trp Arg Leu Thr Lys Leu Ala Arg Leu Leu 
65                  70                  75                  80  


Lys Arg Glu Gly Leu Ile Ala Asp Ala Asn Leu Phe Lys His Lys Ile 
                85                  90                  95      


Glu Lys Asn Thr Pro Val Pro Lys Ser Ser Trp Gln Leu Arg Val Asp 
            100                 105                 110         


Gly Leu Asp Arg Gln Leu Asn Ala Glu Glu Trp Ala Arg Val Ile Tyr 
        115                 120                 125             


His Leu Cys Lys His Arg Gly Phe His Trp Ile Ser Arg Ala Glu Glu 
    130                 135                 140                 


Lys Ala Ala Glu Gly Asp Ser Lys Gly Glu Gly Gly Lys Val Lys Gln 
145                 150                 155                 160 


Gly Leu Ala Gly Thr Lys Lys Leu Met Gln Glu Lys Ser Tyr Arg Thr 
                165                 170                 175     


Ala Ala Glu Met Val Leu Ala Glu Phe Pro Asp Ala Gln Arg Asn Lys 
            180                 185                 190         


Gln Gly Asp Tyr Ser Lys Ala Leu Ser Arg Ile Leu Leu Gly Asp Glu 
        195                 200                 205             


Leu Lys Ala Leu Phe Glu His Gln Arg Lys Leu Gly Asn Pro Val Ala 
    210                 215                 220                 


Thr Glu Lys Leu Glu Ala Ala Ile Leu Gly Asn Gly Asp Lys Lys Cys 
225                 230                 235                 240 


Gly Leu Phe Trp Ala Gln Asn Pro Ala Leu Ala Gly Asn Lys Leu Leu 
                245                 250                 255     


Glu Met Leu Gly Arg Cys Thr Phe Glu Lys Thr Gly Gly Asp Asp Gly 
            260                 265                 270         


Lys Gly Glu Tyr Arg Ala Pro Lys Ala Ser Phe Ser Ala Glu Arg His 
        275                 280                 285             


Val Trp Leu Thr His Leu Asn Asn Leu Arg Ile Val Val Asp Gly Thr 
    290                 295                 300                 


Thr Arg Pro Leu Asn Glu Ala Glu Arg Arg Val Ala Leu Pro Met Pro 
305                 310                 315                 320 


Tyr Gln Gln Ala Gly Asp Leu Ser Tyr Lys Gln Leu Gly Gly Ala Leu 
                325                 330                 335     


Ile Lys Glu Gly Phe Leu Gln Lys Asp Gly Phe Lys Phe Ala Gly Leu 
            340                 345                 350         


Pro Tyr Pro Thr Lys Gln Ala Ser Glu Glu Lys Ala Lys Asp Pro Glu 
        355                 360                 365             


Thr Ser Val Leu Cys Lys Leu Pro Ala Trp His Thr Leu Arg Lys Thr 
    370                 375                 380                 


Leu Glu Lys His Gly Leu Glu Ser Glu Trp Gln Lys Leu Ser Thr Asp 
385                 390                 395                 400 


Ala Leu Asp Gly Lys Pro Gln Leu Leu Asp Asn Ile Ala Trp Val Leu 
                405                 410                 415     


Ser Val Tyr Lys Asp Asp Asp Glu Val Lys Thr Glu Leu Gly Lys Leu 
            420                 425                 430         


Asn Leu Pro Asn Thr Glu Lys Met Ile Asp Ala Leu Leu Asp Ile Arg 
        435                 440                 445             


Phe Asp Lys Phe Ser Asn Leu Ser Leu Lys Ala Leu Ser Lys Ile Val 
    450                 455                 460                 


Pro Phe Met Glu Gln Gly Gln Arg Tyr Asp Glu Ala Val Ala Asn Ile 
465                 470                 475                 480 


Pro Glu Tyr Gly His His Ser Gln Leu His Lys Val Gly Ala Gly Glu 
                485                 490                 495     


His Lys Tyr Leu Pro Pro Phe Tyr Lys Glu Arg Asp Lys Thr Gly Arg 
            500                 505                 510         


Met Val Phe Asn Asp Asp Met Asp Ile Pro Arg Asn Pro Val Val Leu 
        515                 520                 525             


Arg Ala Leu Asn Gln Ala Arg Lys Val Val Asn Ala Leu Ile Lys Glu 
    530                 535                 540                 


Tyr Gly Ser Pro His Glu Val His Ile Glu Met Ala Arg Asp Leu Ser 
545                 550                 555                 560 


Arg Pro Leu Asp Glu Arg Arg Asp Ile Glu Lys Leu Gln Lys Glu Phe 
                565                 570                 575     


Gly Glu Arg Asn Lys Lys Asp Lys Glu Leu Phe Ala Gln Asp Phe Asn 
            580                 585                 590         


Ile Val Gly Thr Val Lys Gly Lys Gln Phe Glu Lys Tyr Gln Leu Tyr 
        595                 600                 605             


Arg Glu Gln Gln Gly Lys Cys Ile Tyr Pro Ser Met Asp Glu Lys Gln 
    610                 615                 620                 


Gly Ile Cys Cys Tyr Ser Leu Lys Pro Leu Asp Leu His Arg Val Leu 
625                 630                 635                 640 


Tyr Glu Glu Gly Tyr Ala Glu Ile Asp His Ala Leu Pro Tyr Ser Arg 
                645                 650                 655     


Ser Phe Asp Asp Ser Lys Asn Asn Arg Val Leu Val Leu Ala Lys Glu 
            660                 665                 670         


Asn Arg Asp Lys Gly Asn Arg Thr Pro Phe Glu Tyr Leu Gly Asp Lys 
        675                 680                 685             


Asp Asn Ser Glu Pro Trp Arg Gln Phe Val Ala Phe Val Glu Gly Asn 
    690                 695                 700                 


Lys Ala Tyr Arg Leu Ala Lys Arg Ser Arg Leu Leu Arg Lys Asp Phe 
705                 710                 715                 720 


Gly Asp Lys Glu Ala Lys Glu Phe Arg Glu Arg Asn Leu Asn Asp Thr 
                725                 730                 735     


Arg Tyr Ile Cys Lys Phe Phe Lys Asn Tyr Val Glu Gln Tyr Leu Gln 
            740                 745                 750         


Leu His Glu Asp Ser Glu Ala Lys Arg Cys Val Val Leu Ser Gly Gln 
        755                 760                 765             


Met Thr Ser Phe Leu Arg Ala Arg Trp Gly Leu Val Lys Val Arg Gly 
    770                 775                 780                 


Asp Ser Asp Arg His His Ala Leu Asp Ala Ala Val Val Ala Ala Cys 
785                 790                 795                 800 


Ser His Gly Met Val Lys Arg Leu Ser Asp Tyr Ser Arg Arg Lys Glu 
                805                 810                 815     


Leu Glu Gln Val Arg Glu Gly Phe Val Asp Val Asp Thr Gly Glu Ile 
            820                 825                 830         


Val Asn Pro Ala Met Phe Gln Gln Val Gln Glu His Phe Pro Asn Pro 
        835                 840                 845             


Trp Pro His Phe Arg Asp Glu Val Lys Leu Arg Leu Ser Ile Asp Asp 
    850                 855                 860                 


Pro Thr Leu Leu Arg Ser Glu Met Glu Lys Phe Gly Thr Tyr Thr Pro 
865                 870                 875                 880 


Glu Ser Leu Glu Ala Leu Arg Pro Leu Phe Val Ser Arg Ala Pro Gln 
                885                 890                 895     


Arg Arg Asn Ser Gly Ala Ala His Lys Asp Thr Ile Tyr Ala Gln Pro 
            900                 905                 910         


Lys Arg Leu Val Ala Gln Gly Gly Val Thr Gln Lys Val Ala Leu Ser 
        915                 920                 925             


Ser Leu Thr Leu Lys Asp Leu Asp Lys Leu Ile Asp Pro His Arg Asn 
    930                 935                 940                 


Glu Arg Leu Tyr Ala Ala Ile Arg Ala Arg Leu Glu Gln His Gly Gly 
945                 950                 955                 960 


Lys Gly Asp Lys Ala Phe Pro Ala Ser Asn Pro Leu Arg Lys Pro Asp 
                965                 970                 975     


Ser Glu Gly Asn Pro Thr Gly Pro Ile Val Arg Gly Val Thr Met Val 
            980                 985                 990         


Ile Asp Lys Leu Ser Gly Ile Pro  Ile Arg Gly Gly Ile  Ala Lys Asn 
        995                 1000                 1005             


Asp Ser  Met Val Arg Val Asp  Val Phe Thr Lys Ala  Asn Lys Phe 
    1010                 1015                 1020             


His Leu  Val Pro Val Tyr Val  His His Ser Val Leu  Gly Leu Pro 
    1025                 1030                 1035             


Asn Gln  Ala Ile Val Gly Gly  Lys Glu Glu Ser Glu  Trp Thr Gln 
    1040                 1045                 1050             


Ile Asp  Asp Ser Phe Asp Trp  His Phe Ser Ile Tyr  Pro Asn Asp 
    1055                 1060                 1065             


Leu Ile  Arg Val Thr Leu Lys  Gln Glu Gly Arg Phe  Gly Tyr Tyr 
    1070                 1075                 1080             


Ala Gly  Cys Asp Arg Ser Thr  Ala Ala Ile His Leu  Trp Ala His 
    1085                 1090                 1095             


Asp Arg  Asn Gln Gln Val Gly  Lys Asp Gly Leu Ile  Arg Ser Ile 
    1100                 1105                 1110             


Gly Val  Lys Thr Ala Leu Ser  Ile Glu Lys Phe Asn  Val Asp Val 
    1115                 1120                 1125             


Leu Gly  Asn Arg Tyr Pro Ala  Pro Pro Glu Lys Arg  Arg Asp Leu 
    1130                 1135                 1140             


Ala 
    


<210>  740
<211>  1145
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  740

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Glu Thr Glu Asn Ser Asn Glu Lys Ser Glu Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Ala Gly Arg Pro Leu Ser Thr Asn Ala Asp Arg Thr Ala Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Lys Asn 
65                  70                  75                  80  


Leu Ile Asp Leu Leu Ile Lys His Arg Leu Ile Asp Lys Asp Thr Pro 
                85                  90                  95      


Leu Thr Glu Ile Gly Lys Asn Thr Thr His Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Arg Glu Arg Ile Glu Leu Glu Asp Leu Ala Arg Val 
        115                 120                 125             


Phe Leu Ala Ile Asn Lys Lys Arg Gly Tyr Arg Ser Ser Arg Lys Val 
    130                 135                 140                 


Asn Asn Glu Glu Glu Gly Gln Val Val Asp Gly Met Ala Val Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Asp Glu Asn Leu Thr Pro Gly Gln Tyr Ala Tyr Glu Leu 
                165                 170                 175     


Leu Ser Lys Gly Lys Lys Tyr Val Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asp Ser Ile Trp Glu Tyr Gln Lys Gln Phe Tyr Ala 
        195                 200                 205             


Asp Ile Leu Asp Asp Glu Leu Tyr Asn Ala Leu Lys Gly Gln Gly Gln 
    210                 215                 220                 


Gln Asn Ser Arg Lys Arg Phe Leu Ala Ile Lys Gly Val Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Lys Arg Asp Glu Val Lys Leu Gln His Tyr Lys Trp 
                245                 250                 255     


Arg Ser Glu Ala Ile Thr Gln Lys Leu Ser Ile Glu Glu Val Ala Tyr 
            260                 265                 270         


Val Leu Val Glu Ile Asn Asn Asp Leu Asn Lys Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Glu Thr 
    290                 295                 300                 


Val Gly Glu Asn Leu Trp Lys Gln Ile Gln Lys Asn Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Glu Thr Gln Ala Gln Phe His Pro Gln Leu Thr Leu Ala 
            340                 345                 350         


Leu Lys Glu Gln Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys Gly Leu Leu Ser Phe Cys Gln Phe Glu Ser Trp Glu 
    370                 375                 380                 


Ile Glu Arg Lys Asp Glu Asn Gly Asn Val Ile Leu Asn Lys Thr Thr 
385                 390                 395                 400 


Gln Leu Pro Lys Arg Gln Thr Val Gly Arg Arg Val Ala Pro Lys Ser 
                405                 410                 415     


Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Asn Ile Asn Asn Leu 
            420                 425                 430         


Glu Ile Ala Lys Ile Ser Asp Gly Asn Ser Lys Asn Lys Lys Glu Thr 
        435                 440                 445             


Glu Ala Leu Ser Asp Asp Glu Arg Lys Leu Leu Phe Glu Glu Leu Asn 
    450                 455                 460                 


Leu Arg Gly Asn Leu Ser Gln Lys Glu Val Leu Gln Ile Leu Gly Leu 
465                 470                 475                 480 


Lys Asp Lys Glu Tyr Lys Thr Asn Phe Pro Glu Gly Leu Glu Gly Asn 
                485                 490                 495     


Arg Thr Asn Ala Ala Leu Phe Asn Ile Tyr Gln Gln Ile Ala Glu Asn 
            500                 505                 510         


Glu Gly Tyr Gly Asp Trp Thr Lys Lys Ser Ala Gln Glu Ile Lys Glu 
        515                 520                 525             


Glu Leu Lys Ala Val Phe Pro Gln Ile Gly Ile Gln Ala Asp Ile Leu 
    530                 535                 540                 


Asp Phe Asn Ala Glu Leu Asp Gly Lys Glu Phe Glu Asn Gln Ala Ser 
545                 550                 555                 560 


Tyr Gln Leu Trp His Leu Leu Tyr Ser Ala Glu Glu Asp Asp Lys Ile 
                565                 570                 575     


Asn Glu Glu Asp Gln Ile Ile Tyr Gly Asn Ser Ala Val Ser Leu Lys 
            580                 585                 590         


Lys Lys Leu Cys Glu Lys Phe Gly Phe Thr Pro Glu Tyr Ala Lys Trp 
        595                 600                 605             


Ile Ala Asn Val Ser Leu Gln Asp Asp Tyr Gly Asn Leu Ser Thr Lys 
    610                 615                 620                 


Ala Met Arg Lys Ile Ile Pro Tyr Leu Ile Asp Gly Asn Asp Tyr Ser 
625                 630                 635                 640 


Glu Ala Cys Ala Leu Ala Gly Tyr Asn His Ser Asn Ser Leu Thr Lys 
                645                 650                 655     


Glu Glu Asn Asp Asn Arg Glu Arg Leu Asn Lys Leu Glu Leu Leu Pro 
            660                 665                 670         


Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Met 
        675                 680                 685             


Val Asn Val Val Asn Gln Val Ile Glu Thr Tyr Gly Lys Pro Asp Glu 
    690                 695                 700                 


Val Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Ser Ala Glu Glu Arg 
705                 710                 715                 720 


Ala Glu Met Thr Lys Gly Ile Asn Glu Ala Thr Arg Arg Asn Glu Asp 
                725                 730                 735     


Ile Lys Lys Leu Ile Thr Lys Asn Phe Gly Ile Pro Asn Pro Thr Lys 
            740                 745                 750         


Asn Asp Val Val Arg Tyr Arg Leu Trp Glu Glu Leu Ala Pro Leu Ala 
        755                 760                 765             


Tyr Lys Asp Val Phe Thr Gly Arg Gln Ile Lys Lys Glu Asp Leu Phe 
    770                 775                 780                 


Ser Ser Lys Ile Asp Ile Glu His Ile Ile Pro Lys Ala Leu Leu Phe 
785                 790                 795                 800 


Asp Asp Ser Phe Ser Asn Lys Thr Leu Ala Phe Arg Glu Thr Asn Leu 
                805                 810                 815     


Lys Lys Ala Asp Arg Thr Ala Tyr Gly Phe Ile Glu Ser Asp Tyr Asn 
            820                 825                 830         


Ala Thr Leu Asp Asp Tyr Ile Gln Arg Val Glu Thr Leu Tyr Asn Asn 
        835                 840                 845             


Ala Lys Gly Thr Ile Ser Lys Gly Lys Arg Asn Lys Leu Leu Met Ala 
    850                 855                 860                 


Gln Lys Asn Leu Pro Asp Gly Phe Ile Glu Arg Asp Leu Arg Asn Ser 
865                 870                 875                 880 


Gln Tyr Ile Ala Lys Lys Ala Lys Ser Met Ile Glu Lys Val Phe Asn 
                885                 890                 895     


Asp Val Asn Val Thr Ser Gly Ser Ile Thr Asp Arg Leu Arg Glu Asp 
            900                 905                 910         


Trp Asp Leu Ile Asn Val Met Lys Glu Leu Asn Phe Pro Lys Tyr Lys 
        915                 920                 925             


Ala Leu Gly Leu Thr Glu Ile Glu Glu Arg Tyr Asp Ile Gly Gln Glu 
    930                 935                 940                 


Lys Thr Lys Lys Val Glu Val Ile Thr Asp Trp Thr Lys Arg Asn Asp 
945                 950                 955                 960 


His Arg His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Thr Lys 
                965                 970                 975     


Ser His Val Gln Tyr Leu Asn Asn Leu Asn Thr Ile His Ser Leu Lys 
            980                 985                 990         


Gly Asp Glu Val Asp Leu Glu Leu  Ser Lys Leu Tyr Gly  Leu Lys Asn 
        995                 1000                 1005             


Thr Ile  Thr Glu Val Val Asn  Lys Lys Arg Lys Phe  Ile Pro Pro 
    1010                 1015                 1020             


Met Pro  Asn Phe Arg Glu Glu  Ala Lys Lys His Ile  Glu Thr Ile 
    1025                 1030                 1035             


Leu Val  Ser Ile Lys Asn Lys  Asn Lys Val Tyr Thr  Lys Asn Ile 
    1040                 1045                 1050             


Asn Lys  Thr Lys Lys Glu Ser  Gly Tyr His Thr Lys  Glu Gln Leu 
    1055                 1060                 1065             


Thr Pro  Arg Gly Gln Leu His  Lys Glu Thr Val Tyr  Gly Lys Ser 
    1070                 1075                 1080             


Lys Arg  Pro Met Asn Lys Pro  Thr Lys Ile Asn Lys  Asn Phe Ser 
    1085                 1090                 1095             


Leu Glu  Gln Ala Gln Leu Ile  Ile Asn Val Gln Glu  Lys Glu Leu 
    1100                 1105                 1110             


Val Leu  Arg His Leu Ala Gln  Phe Asp Asn Asn Pro  Ala Val Ala 
    1115                 1120                 1125             


Phe Asp  Thr Lys Thr Leu Lys  Lys Cys His Tyr Leu  Lys Met Val 
    1130                 1135                 1140             


Asn Leu  
    1145 


<210>  741
<211>  1146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  741

Met Ser Gly Asn Thr His Gly Pro Leu Thr Phe Gly Phe Asp Ile Gly 
1               5                   10                  15      


Ile Ala Ser Val Gly Trp Cys Val Leu Gly Glu Asn Arg Ile Val Asn 
            20                  25                  30          


Leu Gly Val Arg Cys Phe Asp Lys Ala Glu Thr Ala Lys Glu Gly Glu 
        35                  40                  45              


Ser Leu Asn Leu Ala Arg Arg Thr Ala Arg Leu Met Arg Arg Arg Leu 
    50                  55                  60                  


Arg Arg Arg Ala Trp Arg Leu Thr Lys Leu Ala Arg Leu Leu Lys Arg 
65                  70                  75                  80  


Glu Gly Leu Ile Ala Glu Ala Ser Arg Ile His Ile Pro Ser Pro Val 
                85                  90                  95      


Ser Pro Trp Arg Leu Arg Val Glu Gly Ile Asp Arg Arg Leu Thr Asp 
            100                 105                 110         


Asp Glu Trp Ala Arg Val Ile Tyr His Leu Cys Lys His Arg Gly Phe 
        115                 120                 125             


His Trp Ile Ser Arg Ala Glu Glu Arg Lys Ala Glu Ser Asp Ala Lys 
    130                 135                 140                 


Glu Gly Gly Arg Val Lys Lys Gly Leu Ser Asp Thr Gln Arg Arg Met 
145                 150                 155                 160 


Thr Glu Lys Gly Tyr Arg Ser Ala Ala Glu Met Val Leu Ala Glu Phe 
                165                 170                 175     


Pro Asp Ala Gln Arg Asn Lys Gln Gly Asp Tyr Gly Lys Ala Leu Ser 
            180                 185                 190         


Arg Ile Leu Leu Ser Asp Glu Leu Ala Leu Leu Phe Arg Arg Gln Arg 
        195                 200                 205             


Glu Leu Gly Asn Pro Asn Ala Gly Asn Glu Leu Glu Ala Thr Ile Leu 
    210                 215                 220                 


Gly Asn Gly Asn Arg Lys Ser Gly Leu Phe Trp Glu Gln Lys Pro Pro 
225                 230                 235                 240 


Leu Ala Gly Ala Asp Leu Leu Lys Met Leu Gly Arg Cys Thr Phe Glu 
                245                 250                 255     


Lys Asn Glu Tyr Arg Ala Pro Lys Ala Ser Phe Thr Ala Glu Arg His 
            260                 265                 270         


Val Trp Leu Thr Arg Leu Asn Asn Leu Arg Val Val Val Asp Gly Thr 
        275                 280                 285             


Thr Arg Ser Leu Asp Asp Ala Glu His Arg Leu Val Leu Pro Leu Pro 
    290                 295                 300                 


Tyr Lys Gln Ala Gly Asp Phe Thr Tyr Lys Gln Leu Arg Thr Ala Leu 
305                 310                 315                 320 


Thr Lys Ala Gly Leu Leu Pro Glu Gly Phe Lys Phe Ala Gly Leu Ser 
                325                 330                 335     


Tyr Pro Thr Gly Arg Gln Leu Asp Asp Pro Lys Ala Lys Asp Pro Glu 
            340                 345                 350         


Ser Glu Lys Leu Val Arg Leu Pro Ala Trp Gln Glu Leu Arg Lys Thr 
        355                 360                 365             


Leu Lys Asp Lys Asn Leu Glu Thr Glu Trp Glu Gly Met Ala Gly Ala 
    370                 375                 380                 


Ala Thr Gly Gly Asn Pro Glu Leu Leu Asp Glu Ile Ala Arg Val Leu 
385                 390                 395                 400 


Ser Val Phe Lys Asp Gly Asp Glu Val Thr Ala Glu Leu Trp Lys Leu 
                405                 410                 415     


Pro Leu Pro Gly Gly Lys Ala Met Ile Asp Ala Leu Ser Glu Ile Ser 
            420                 425                 430         


Phe Asp Lys Phe His Ala Leu Ser Leu Lys Ala Leu Arg Lys Ile Val 
        435                 440                 445             


Pro His Met Glu Ser Gly Leu Arg Tyr Asp Glu Ala Cys Ala Val Ala 
    450                 455                 460                 


Gly Tyr His His Ser Gln Leu His Ala Ala Gly Ala Gly Glu His Lys 
465                 470                 475                 480 


Phe Leu Pro Pro Phe Tyr Ser Arg Arg Asp Lys Asp Gly Arg Met Val 
                485                 490                 495     


Phe Asn Glu Glu Leu Asp Val Pro Arg Asn Pro Val Val Leu Arg Ala 
            500                 505                 510         


Leu Asn Gln Ala Arg Lys Val Leu Asn Ala Leu Val Arg Thr Tyr Gly 
        515                 520                 525             


Ser Pro Thr Ala Val His Ile Glu Met Ala Arg Asp Leu Ser Arg Pro 
    530                 535                 540                 


Leu Asp Glu Arg Asn Lys Val Lys Lys Ala Gln Asp Glu Tyr Arg Glu 
545                 550                 555                 560 


Arg Asn Glu Lys Asp Lys Ala Ala Phe Ala Ala Glu Tyr Gly Ile Ala 
                565                 570                 575     


Pro Lys Ser Arg Glu Phe Glu Lys Phe Gln Leu Tyr Arg Glu Gln Gln 
            580                 585                 590         


Gly Lys Cys Ala Tyr Ser Leu Lys Ser Leu Asp Leu His Arg Val Leu 
        595                 600                 605             


His Asp Ile Gly Tyr Ala Glu Val Asp His Ala Leu Pro Tyr Ser Arg 
    610                 615                 620                 


Ser Tyr Asp Asp Ser Lys Asn Asn Lys Val Leu Ala Leu Thr Glu Glu 
625                 630                 635                 640 


Asn Arg Asn Lys Gly Asn Arg Thr Ala Tyr Glu Tyr Leu Thr Ser Phe 
                645                 650                 655     


Pro Gly Gly Glu Asn Gly Glu Arg Trp Arg Asn Phe Ser Val Ala Val 
            660                 665                 670         


Glu Thr Asn Lys Ala Tyr Arg Leu Ala Lys Arg Thr Arg Leu Leu Arg 
        675                 680                 685             


Lys Asp Phe Ser Gly Arg Ala Ala Glu Glu Phe Arg Glu Arg Asn Leu 
    690                 695                 700                 


Asn Asp Thr Arg Tyr Ile Cys Arg Phe Phe Lys Asn Thr Val Glu Arg 
705                 710                 715                 720 


Phe Leu Lys Leu Asp Ala Lys Val Gly Ala Gly Asp Thr Ala Glu Ile 
                725                 730                 735     


Ser Ala Lys Arg Cys Val Val Leu Ser Gly Gln Thr Thr Ala Phe Leu 
            740                 745                 750         


Arg Ala Arg Trp Gly Leu Leu Lys Val Arg Gly Asn Ser Asp Arg His 
        755                 760                 765             


His Ala Leu Asp Ala Ala Val Val Ala Ala Cys Ser His Gly Met Val 
    770                 775                 780                 


Lys Arg Leu Gly Asp Tyr Ser Arg Thr Lys Glu Leu Ala Lys Val His 
785                 790                 795                 800 


Asp Gly Phe Pro Asp Pro Glu Thr Gly Glu Ile Ile Asp Pro Val Met 
                805                 810                 815     


Phe Gln Gln Leu His Ala His Phe Pro Asp Pro Trp Pro His Phe Arg 
            820                 825                 830         


Ala Glu Leu Glu Ala Arg Leu Asn Val Asp Asp Thr Ala Asp Leu Arg 
        835                 840                 845             


Ala Arg Met Glu Leu Phe Gly Thr Tyr Ala Pro Glu Ala Leu Glu Thr 
    850                 855                 860                 


Leu Arg Pro Leu Phe Val Ser Arg Ala Pro Gln Arg Arg Asn Ser Gly 
865                 870                 875                 880 


Ala Ala His Lys Asp Thr Ile Tyr Ala Gln Pro Glu Arg Leu Lys Glu 
                885                 890                 895     


Gln Gly Gly Val Thr Gln Lys Val Pro Leu Thr Ser Leu Thr Leu Lys 
            900                 905                 910         


Asp Leu Asp Lys Leu Ile Asp Pro His Arg Asn Glu Lys Leu Tyr Ala 
        915                 920                 925             


Ala Ile Arg Ala Arg Leu Glu Ala His Gly Gly Lys Ala Asp Lys Ala 
    930                 935                 940                 


Phe Pro Ala Gly Asn Pro Leu Arg Lys Pro Asp Arg Glu Gly Asn Pro 
945                 950                 955                 960 


Thr Gly Pro Val Val Arg Thr Val Thr Met Val Ile Asp Lys Leu Ser 
                965                 970                 975     


Gly Ile Pro Val Arg Gly Gly Ile Ala Lys Asn Asp Thr Met Leu Arg 
            980                 985                 990         


Val Asp Ile Phe Arg His Lys Lys  Asp Gly Lys Phe His  Leu Val Pro 
        995                 1000                 1005             


Val Tyr  Val Tyr His Ala Val  Ala Lys Glu Phe Pro  Asn Arg Ala 
    1010                 1015                 1020             


Ile Val  Gln Ala Lys Asp Glu  Asp Glu Trp Thr Leu  Ile Asp Asp 
    1025                 1030                 1035             


Lys Phe  Asp Phe Cys Phe Ser  Ala His Pro Asn Asp  Leu Leu Lys 
    1040                 1045                 1050             


Ile Thr  Gln Lys Ser Gly Glu  Thr Ile Phe Gly Tyr  Tyr Arg Gly 
    1055                 1060                 1065             


Cys His  Ser Gly Thr Ala Ala  Ile Asn Ile Ala Leu  His Asp Arg 
    1070                 1075                 1080             


Tyr Ala  Tyr Ala Glu Lys Thr  Thr Ser Leu Ala Phe  Ser Lys Lys 
    1085                 1090                 1095             


Asn Pro  Gly Lys Pro Ile Pro  Asn Asp Lys Leu Gly  Leu Ile Glu 
    1100                 1105                 1110             


Gly Ile  Gly Val Lys Met Ala  Ala Asn Met Glu Lys  Phe His Val 
    1115                 1120                 1125             


Asp Thr  Leu Gly Asn Ile Tyr  Pro Ala Pro Pro Glu  Gln Arg Arg 
    1130                 1135                 1140             


Gly Leu  Ala 
    1145     


<210>  742
<211>  1146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  742

Met Ala Tyr Arg Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Val Ala Leu Glu Lys Asp Glu Ser Gly Leu Lys Pro Val Arg 
            20                  25                  30          


Ile Gln Asp Leu Gly Val Arg Ile Phe Asp Lys Ala Glu Asp Ser Lys 
        35                  40                  45              


Thr Gly Ala Ser Leu Ala Leu Pro Arg Arg Glu Ala Arg Ser Ala Arg 
    50                  55                  60                  


Arg Arg Thr Arg Arg Arg Arg His Arg Leu Trp Arg Val Lys Arg Leu 
65                  70                  75                  80  


Leu Glu Gln His Gly Ile Leu Ser Met Glu Gln Ile Glu Ala Leu Tyr 
                85                  90                  95      


Ala Gln Arg Thr Ser Ser Pro Asp Val Tyr Ala Leu Arg Val Ala Gly 
            100                 105                 110         


Leu Asp Arg Cys Leu Ile Ala Glu Glu Ile Ala Arg Val Leu Ile His 
        115                 120                 125             


Ile Ala His Arg Arg Gly Phe Gln Ser Asn Arg Lys Ser Glu Ile Lys 
    130                 135                 140                 


Asp Ser Asp Ala Gly Lys Leu Leu Lys Ala Val Gln Glu Asn Glu Asn 
145                 150                 155                 160 


Leu Met Gln Ser Lys Gly Tyr Arg Thr Val Ala Glu Met Leu Val Ser 
                165                 170                 175     


Glu Ala Thr Lys Thr Asp Ala Glu Gly Lys Leu Val His Gly Lys Lys 
            180                 185                 190         


His Gly Tyr Val Ser Asn Val Arg Asn Lys Ala Gly Glu Tyr Arg His 
        195                 200                 205             


Thr Val Ser Arg Gln Ala Ile Val Asp Glu Val Arg Lys Ile Phe Ala 
    210                 215                 220                 


Ala Gln Arg Ala Leu Gly Asn Asp Val Met Ser Glu Glu Leu Glu Asp 
225                 230                 235                 240 


Ser Tyr Leu Lys Ile Leu Cys Ser Gln Arg Asn Phe Asp Asp Gly Pro 
                245                 250                 255     


Gly Gly Asp Ser Pro Tyr Gly His Gly Ser Val Ser Pro Asp Gly Val 
            260                 265                 270         


Arg Gln Ser Ile Tyr Glu Arg Met Val Gly Ser Cys Thr Phe Glu Thr 
        275                 280                 285             


Gly Glu Lys Arg Ala Pro Arg Ser Ser Tyr Ser Phe Glu Arg Phe Gln 
    290                 295                 300                 


Leu Leu Thr Lys Val Val Asn Leu Arg Ile Tyr Arg Gln Gln Glu Asp 
305                 310                 315                 320 


Gly Gly Arg Tyr Pro Cys Glu Leu Thr Gln Thr Glu Arg Ala Arg Val 
                325                 330                 335     


Ile Asp Cys Ala Tyr Glu Gln Thr Lys Ile Thr Tyr Gly Lys Leu Arg 
            340                 345                 350         


Lys Leu Leu Asp Met Lys Asp Thr Glu Ser Phe Ala Gly Leu Thr Tyr 
        355                 360                 365             


Gly Leu Asn Arg Ser Arg Asn Lys Thr Glu Asp Thr Val Phe Val Glu 
    370                 375                 380                 


Met Lys Phe Tyr His Glu Val Arg Lys Ala Leu Gln Arg Ala Gly Val 
385                 390                 395                 400 


Phe Ile Gln Asp Leu Ser Ile Glu Thr Leu Asp Gln Ile Gly Trp Ile 
                405                 410                 415     


Leu Ser Val Trp Lys Ser Asp Asp Asn Arg Arg Lys Lys Leu Ser Thr 
            420                 425                 430         


Leu Gly Leu Ser Asp Asn Val Ile Glu Glu Leu Leu Pro Leu Asn Gly 
        435                 440                 445             


Ser Lys Phe Gly His Leu Ser Leu Lys Ala Ile Arg Lys Ile Leu Pro 
    450                 455                 460                 


Phe Leu Glu Asp Gly Tyr Ser Tyr Asp Val Ala Cys Glu Leu Ala Gly 
465                 470                 475                 480 


Tyr Gln Phe Gln Gly Lys Thr Glu Tyr Val Lys Gln Arg Leu Leu Pro 
                485                 490                 495     


Pro Leu Gly Glu Gly Glu Val Thr Asn Pro Val Val Arg Arg Ala Leu 
            500                 505                 510         


Ser Gln Ala Ile Lys Val Val Asn Ala Val Ile Arg Lys His Gly Ser 
        515                 520                 525             


Pro Glu Ser Ile His Ile Glu Leu Ala Arg Glu Leu Ser Lys Asn Leu 
    530                 535                 540                 


Asp Glu Arg Arg Lys Ile Glu Lys Ala Gln Lys Glu Asn Gln Lys Asn 
545                 550                 555                 560 


Asn Glu Gln Ile Lys Asp Glu Ile Arg Glu Ile Leu Gly Ser Ala His 
                565                 570                 575     


Val Thr Gly Arg Asp Ile Val Lys Tyr Lys Leu Phe Lys Gln Gln Gln 
            580                 585                 590         


Glu Phe Cys Met Tyr Ser Gly Glu Lys Leu Asp Val Thr Arg Leu Phe 
        595                 600                 605             


Glu Pro Gly Tyr Ala Glu Val Asp His Ile Ile Pro Tyr Gly Ile Ser 
    610                 615                 620                 


Phe Asp Asp Ser Tyr Asp Asn Lys Val Leu Val Lys Thr Glu Gln Asn 
625                 630                 635                 640 


Arg Gln Lys Gly Asn Arg Thr Pro Leu Glu Tyr Leu Arg Asp Lys Pro 
                645                 650                 655     


Glu Gln Lys Ala Lys Phe Ile Ala Leu Val Glu Ser Ile Pro Leu Ser 
            660                 665                 670         


Gln Lys Lys Lys Asn His Leu Leu Met Asp Lys Arg Ala Ile Asp Leu 
        675                 680                 685             


Glu Gln Glu Gly Phe Arg Glu Arg Asn Leu Ser Asp Thr Arg Tyr Ile 
    690                 695                 700                 


Thr Arg Ala Leu Met Asn His Ile Gln Ala Trp Leu Leu Phe Asp Glu 
705                 710                 715                 720 


Thr Ala Ser Thr Arg Ser Lys Arg Val Val Cys Val Asn Gly Ala Val 
                725                 730                 735     


Thr Ala Tyr Met Arg Ala Arg Trp Gly Leu Thr Lys Asp Arg Asp Ala 
            740                 745                 750         


Gly Asp Lys His His Ala Ala Asp Ala Val Val Val Ala Cys Ile Gly 
        755                 760                 765             


Asp Ser Leu Ile Gln Arg Val Thr Lys Tyr Asp Lys Phe Lys Arg Asn 
    770                 775                 780                 


Ala Leu Ala Asp Arg Asn Arg Tyr Val Gln Gln Val Ser Lys Ser Glu 
785                 790                 795                 800 


Gly Ile Thr Gln Tyr Val Asp Lys Glu Thr Gly Glu Val Phe Thr Trp 
                805                 810                 815     


Glu Ser Phe Asp Glu Arg Lys Phe Leu Pro Asn Glu Pro Leu Glu Pro 
            820                 825                 830         


Trp Pro Phe Phe Arg Asp Glu Leu Leu Ala Arg Leu Ser Asp Asp Pro 
        835                 840                 845             


Ser Lys Asn Ile Arg Ala Ile Gly Leu Leu Thr Tyr Ser Glu Thr Glu 
    850                 855                 860                 


Gln Ile Asp Pro Ile Phe Val Ser Arg Met Pro Thr Arg Lys Val Thr 
865                 870                 875                 880 


Gly Ala Ala His Lys Glu Thr Ile Arg Ser Pro Arg Ile Val Lys Val 
                885                 890                 895     


Asp Asp Asn Lys Gly Thr Glu Ile Gln Val Val Val Ser Lys Val Ala 
            900                 905                 910         


Leu Thr Glu Leu Lys Leu Thr Lys Asp Gly Glu Ile Lys Asp Tyr Phe 
        915                 920                 925             


Arg Pro Glu Asp Asp Pro Arg Leu Tyr Asn Thr Leu Arg Glu Arg Leu 
    930                 935                 940                 


Val Gln Phe Gly Gly Asp Ala Lys Ala Ala Phe Lys Glu Pro Val Tyr 
945                 950                 955                 960 


Lys Ile Ser Lys Asp Gly Ser Val Arg Thr Pro Val Arg Lys Val Lys 
                965                 970                 975     


Ile Gln Glu Lys Leu Thr Leu Gly Val Pro Val His Gly Gly Arg Gly 
            980                 985                 990         


Ile Ala Glu Asn Gly Gly Met Val  Arg Ile Asp Val Phe  Ala Lys Gly 
        995                 1000                 1005             


Gly Lys  Tyr Tyr Phe Val Pro  Ile Tyr Val Ala Asp  Val Leu Lys 
    1010                 1015                 1020             


Arg Glu  Leu Pro Asn Arg Leu  Ala Thr Ala His Lys  Pro Tyr Ser 
    1025                 1030                 1035             


Glu Trp  Arg Val Val Asp Asp  Ser Tyr Gln Phe Lys  Phe Ser Leu 
    1040                 1045                 1050             


Tyr Pro  Asn Asp Ala Val Met  Ile Lys Pro Ser Arg  Glu Val Asp 
    1055                 1060                 1065             


Ile Thr  Tyr Lys Asp Arg Lys  Glu Pro Val Gly Cys  Arg Ile Met 
    1070                 1075                 1080             


Tyr Phe  Val Ser Ala Asn Ile  Ala Ser Ala Ser Ile  Ser Leu Arg 
    1085                 1090                 1095             


Thr His  Asp Asn Ser Gly Glu  Leu Glu Gly Leu Gly  Ile Gln Gly 
    1100                 1105                 1110             


Leu Glu  Val Phe Glu Lys Tyr  Val Val Gly Pro Leu  Gly Asp Thr 
    1115                 1120                 1125             


His Pro  Val Tyr Lys Glu Arg  Arg Met Pro Phe Arg  Val Glu Arg 
    1130                 1135                 1140             


Lys Met  Asn 
    1145     


<210>  743
<211>  1146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  743

Met Ala Tyr Arg Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Val Ala Leu Glu Lys Asp Glu Ser Gly Leu Lys Pro Val Arg 
            20                  25                  30          


Ile Gln Asp Leu Gly Val Arg Ile Phe Asp Lys Ala Glu Asp Ser Lys 
        35                  40                  45              


Thr Gly Ala Ser Leu Ala Leu Pro Arg Arg Glu Ala Arg Ser Ala Arg 
    50                  55                  60                  


Arg Arg Thr Arg Arg Arg Arg His Arg Leu Trp Arg Val Lys Arg Leu 
65                  70                  75                  80  


Leu Glu Gln His Gly Ile Leu Ser Met Glu Gln Ile Glu Ala Leu Tyr 
                85                  90                  95      


Ala Gln Arg Thr Ser Ser Pro Asp Val Tyr Ala Leu Arg Val Ala Gly 
            100                 105                 110         


Leu Asp Arg Cys Leu Ile Ala Glu Glu Ile Ala Arg Val Leu Ile His 
        115                 120                 125             


Ile Ala His Arg Arg Gly Phe Gln Ser Asn Arg Lys Ser Glu Ile Lys 
    130                 135                 140                 


Asp Ser Asp Ala Gly Lys Leu Leu Lys Ala Val Gln Glu Asn Glu Asn 
145                 150                 155                 160 


Leu Met Gln Ser Lys Gly Tyr Arg Thr Val Ala Glu Met Leu Val Ser 
                165                 170                 175     


Glu Ala Thr Lys Thr Asp Ala Glu Gly Lys Leu Val His Gly Lys Lys 
            180                 185                 190         


His Gly Tyr Val Ser Asn Val Arg Asn Lys Ala Gly Glu Tyr Arg His 
        195                 200                 205             


Thr Val Ser Arg Gln Ala Ile Val Asp Glu Val Arg Lys Ile Phe Ala 
    210                 215                 220                 


Ala Gln Arg Ala Leu Gly Asn Asp Val Met Ser Glu Glu Leu Glu Asp 
225                 230                 235                 240 


Ser Tyr Leu Lys Ile Leu Cys Ser Gln Arg Asn Phe Asp Asp Gly Pro 
                245                 250                 255     


Gly Gly Asp Ser Pro Tyr Gly His Gly Ser Val Ser Pro Asp Gly Val 
            260                 265                 270         


Arg Gln Ser Ile Tyr Glu Arg Met Val Gly Ser Cys Thr Phe Glu Thr 
        275                 280                 285             


Gly Glu Lys Arg Ala Pro Arg Ser Ser Tyr Ser Phe Glu Arg Phe Gln 
    290                 295                 300                 


Leu Leu Thr Lys Val Val Asn Leu Arg Ile Tyr Arg Gln Gln Glu Asp 
305                 310                 315                 320 


Gly Gly Arg Tyr Pro Cys Glu Leu Thr Gln Thr Glu Arg Ala Arg Val 
                325                 330                 335     


Ile Asp Cys Ala Tyr Glu Gln Thr Lys Ile Thr Tyr Gly Lys Leu Arg 
            340                 345                 350         


Lys Leu Leu Asp Met Lys Asp Thr Glu Ser Phe Ala Gly Leu Thr Tyr 
        355                 360                 365             


Gly Leu Asn Arg Ser Arg Asn Lys Thr Glu Asp Thr Val Phe Val Glu 
    370                 375                 380                 


Met Lys Phe Tyr His Glu Val Arg Lys Ala Leu Gln Arg Ala Gly Val 
385                 390                 395                 400 


Phe Ile Gln Asp Leu Ser Ile Glu Thr Leu Asp Gln Ile Gly Trp Ile 
                405                 410                 415     


Leu Ser Val Trp Lys Ser Asp Asp Asn Arg Arg Lys Lys Leu Ser Thr 
            420                 425                 430         


Leu Gly Leu Ser Asp Asn Val Ile Glu Glu Leu Leu Pro Leu Asn Gly 
        435                 440                 445             


Ser Lys Phe Gly His Leu Ser Leu Lys Ala Ile Arg Lys Ile Leu Pro 
    450                 455                 460                 


Phe Leu Glu Asp Gly Tyr Ser Tyr Asp Val Ala Cys Glu Leu Ala Gly 
465                 470                 475                 480 


Tyr Gln Phe Gln Gly Lys Thr Glu Tyr Val Lys Gln Arg Leu Leu Pro 
                485                 490                 495     


Pro Leu Gly Glu Gly Glu Val Thr Asn Pro Val Val Arg Arg Ala Leu 
            500                 505                 510         


Ser Gln Ala Ile Lys Val Val Asn Ala Val Ile Arg Lys His Gly Ser 
        515                 520                 525             


Pro Glu Ser Ile His Ile Glu Leu Ala Arg Glu Leu Ser Lys Asn Leu 
    530                 535                 540                 


Asp Glu Arg Arg Lys Ile Glu Lys Ala Gln Lys Glu Asn Gln Lys Asn 
545                 550                 555                 560 


Asn Glu Gln Ile Lys Asp Glu Ile Arg Glu Ile Leu Gly Ser Ala His 
                565                 570                 575     


Val Thr Gly Arg Asp Ile Val Lys Tyr Lys Leu Phe Lys Gln Gln Gln 
            580                 585                 590         


Glu Phe Cys Met Tyr Ser Gly Glu Lys Leu Asp Val Thr Arg Leu Phe 
        595                 600                 605             


Glu Pro Gly Tyr Ala Glu Val Asp His Ile Ile Pro Tyr Gly Ile Ser 
    610                 615                 620                 


Phe Asp Asp Ser Tyr Asp Asn Lys Val Leu Val Lys Thr Glu Gln Asn 
625                 630                 635                 640 


Arg Gln Lys Gly Asn Arg Thr Pro Leu Glu Tyr Leu Arg Asp Lys Pro 
                645                 650                 655     


Glu Gln Lys Ala Lys Phe Ile Ala Leu Val Glu Ser Ile Pro Leu Ser 
            660                 665                 670         


Gln Lys Lys Lys Asn His Leu Leu Met Asp Lys Arg Ala Ile Asp Leu 
        675                 680                 685             


Glu Gln Glu Gly Phe Arg Glu Arg Asn Leu Ser Asp Thr Arg Tyr Ile 
    690                 695                 700                 


Thr Arg Ala Leu Met Asn His Ile Gln Ala Trp Leu Leu Phe Asp Glu 
705                 710                 715                 720 


Thr Ala Ser Thr Arg Ser Lys Arg Val Val Cys Val Asn Gly Ala Val 
                725                 730                 735     


Thr Ala Tyr Met Arg Ala Arg Trp Gly Leu Thr Lys Asp Arg Asn Ala 
            740                 745                 750         


Gly Asp Lys His His Ala Ala Asp Ala Val Val Val Ala Cys Ile Gly 
        755                 760                 765             


Asp Ser Leu Ile Gln Arg Val Thr Lys Tyr Asp Lys Phe Lys Arg Asn 
    770                 775                 780                 


Ala Leu Ala Asp Arg Asn Arg Tyr Val Gln Gln Val Ser Lys Ser Glu 
785                 790                 795                 800 


Gly Ile Thr Gln Tyr Val Asp Lys Glu Thr Gly Glu Val Phe Thr Trp 
                805                 810                 815     


Glu Ser Phe Asp Glu Arg Lys Phe Leu Pro Asn Glu Pro Leu Glu Pro 
            820                 825                 830         


Trp Pro Phe Phe Arg Asp Glu Leu Leu Ala Arg Leu Ser Asp Asp Pro 
        835                 840                 845             


Ser Lys Asn Ile Arg Ala Ile Gly Leu Leu Thr Tyr Ser Glu Thr Glu 
    850                 855                 860                 


Gln Ile Asp Pro Ile Phe Val Ser Arg Met Pro Thr Arg Lys Val Thr 
865                 870                 875                 880 


Gly Ala Ala His Lys Glu Thr Ile Arg Ser Pro Arg Ile Val Lys Val 
                885                 890                 895     


Asp Asp Asn Lys Gly Thr Glu Ile Gln Val Val Val Ser Lys Val Ala 
            900                 905                 910         


Leu Thr Glu Leu Lys Leu Thr Lys Asp Gly Glu Ile Lys Asp Tyr Phe 
        915                 920                 925             


Arg Pro Glu Asp Asp Pro Arg Leu Tyr Asn Thr Leu Arg Glu Arg Leu 
    930                 935                 940                 


Val Gln Phe Gly Gly Asp Ala Lys Ala Ala Phe Lys Glu Pro Val Tyr 
945                 950                 955                 960 


Lys Ile Ser Lys Asp Gly Ser Val Arg Thr Pro Val Arg Lys Val Lys 
                965                 970                 975     


Ile Gln Glu Lys Leu Thr Leu Gly Val Pro Val His Gly Gly Arg Gly 
            980                 985                 990         


Ile Ala Glu Asn Gly Gly Met Val  Arg Ile Asp Val Phe  Ala Lys Asp 
        995                 1000                 1005             


Gly Lys  Tyr Tyr Phe Val Pro  Ile Tyr Val Ala Asp  Val Leu Lys 
    1010                 1015                 1020             


Arg Glu  Leu Pro Asn Arg Leu  Ala Thr Ala His Lys  Pro Tyr Ser 
    1025                 1030                 1035             


Glu Trp  Arg Val Val Asp Asp  Ser Tyr Gln Phe Lys  Phe Ser Leu 
    1040                 1045                 1050             


Tyr Pro  Asn Asp Ala Val Met  Ile Lys Pro Ser Arg  Glu Val Asp 
    1055                 1060                 1065             


Ile Thr  Tyr Lys Asp Arg Lys  Glu Pro Val Gly Leu  Arg Leu Met 
    1070                 1075                 1080             


Tyr Phe  Ala Gly Ala Asp Ile  Ser Gly Ala Thr Leu  Thr Leu Arg 
    1085                 1090                 1095             


Thr His  Asp Asn Ser Gly Glu  Leu Lys Gly Leu Gly  Ile Gln Gly 
    1100                 1105                 1110             


Leu Glu  Val Phe Glu Lys Tyr  Val Val Gly Pro Leu  Gly Asp Thr 
    1115                 1120                 1125             


His Pro  Val Tyr Lys Glu Arg  Arg Met Pro Phe Arg  Val Glu Arg 
    1130                 1135                 1140             


Lys Met  Asn 
    1145     


<210>  744
<211>  1148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  744

Met Thr Lys Leu Asn Gln Pro Tyr Gly Ile Gly Leu Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Val Val Asp Ala Asn Ser His Leu Leu Arg 
            20                  25                  30          


Leu Lys Gly Glu Thr Ala Ile Gly Ala Arg Leu Phe Arg Glu Gly Gln 
        35                  40                  45              


Ser Ala Ala Asp Arg Arg Gly Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Thr Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Ala Pro His 
65                  70                  75                  80  


Ile Thr Lys Ile Asp Pro Asp Phe Phe Leu Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Asp Arg Phe Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Glu Ala Tyr Pro Ser Met Tyr 
        115                 120                 125             


His Leu Arg Leu Arg Leu Met Thr Asp Ala Ser Gln Ala Asp Pro Arg 
    130                 135                 140                 


Glu Ile Phe Leu Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Ala Ala Lys Asp Phe Asn Thr Asp Lys Val Asp Leu 
                165                 170                 175     


Glu Asp Ile Phe Pro Ala Leu Thr Glu Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Glu Leu Thr Phe Asp Leu Ala Lys Ala Asp Asp Phe Lys Ala 
        195                 200                 205             


Lys Leu Leu Asp Glu Gln Ala Thr Pro Ser Asp Thr Gln Lys Ala Leu 
    210                 215                 220                 


Val Asn Leu Leu Leu Ser Ser Asp Gly Glu Lys Glu Ile Val Lys Lys 
225                 230                 235                 240 


Arg Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Ile Thr Gly Leu Lys 
                245                 250                 255     


Thr Lys Phe Asn Leu Ala Leu Gly Thr Glu Val Asp Glu Ala Asp Ala 
            260                 265                 270         


Ser Asn Trp Gln Phe Ser Met Gly Gln Leu Asp Asp Lys Trp Ser Asn 
        275                 280                 285             


Ile Glu Thr Ser Met Thr Asp Gln Gly Thr Glu Ile Phe Glu Gln Ile 
    290                 295                 300                 


Gln Glu Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Met Ser Leu Ser Gln Ala Lys Val Ala Asp Tyr Gly Gln His Asn Glu 
                325                 330                 335     


Asp Leu Glu Leu Phe Lys Thr Tyr Leu Lys Lys Leu Asn Asp His Glu 
            340                 345                 350         


Leu Ala Lys Thr Ile Arg Val Ile Tyr Asp Arg Tyr Ile Asn Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Leu Arg Glu Asp Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Asn Glu Val Ser Glu Gln Leu Leu Asn Arg 
385                 390                 395                 400 


Met Gly Gln Ala Asn Phe Met Leu Lys Gln Arg Thr Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Leu Gln Gln Arg Glu Leu Asp Gln Ile Ile Ala 
            420                 425                 430         


Asn Gln Ser Lys Tyr Tyr Asp Trp Leu Ala Lys Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Ala Ser Lys Pro Tyr Gln Leu Asp Glu Leu Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ile Thr Pro Lys Glu Gln Ala 
465                 470                 475                 480 


Lys Ser Gly Glu Asn Val Phe Ala Trp Met Val Arg Lys Asp Pro Ser 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Val Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Lys Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asn Glu Ala Leu 
545                 550                 555                 560 


Gly Thr Asp Gln Lys Gln Arg Leu Ile Arg Glu Val Phe Glu Arg His 
                565                 570                 575     


Ser Ser Val Thr Ile Lys Gln Val Ala Asp Asn Leu Val Ala His Gly 
            580                 585                 590         


Asp Phe Ala Gln Arg Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr His Gln Leu Lys Glu Ile Leu His 
    610                 615                 620                 


Gly Ala Ile Asp Asp Ser Thr Lys Leu His Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Thr Trp Ser Thr Val Phe Glu Asp Ala Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Ala Glu Ile Asp Trp Leu Asp Ala Ala Thr Ile Lys Lys Leu Ser Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Arg Lys Leu Leu Asp Gly 
        675                 680                 685             


Leu Lys Leu Gly Asn Gly Gln Thr Val Ile Gln Glu Leu Met Leu Ser 
    690                 695                 700                 


Asn His Asn Leu Met Gln Ile Leu Thr Asp Glu Thr Leu Lys Thr Thr 
705                 710                 715                 720 


Met Ala Glu Leu Asn Gln Asp Lys Leu Lys Ala Asp Asp Ile Glu Asp 
                725                 730                 735     


Val Ile Asn Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Arg Val Val Glu Asp Ile Lys His Ala Ala Asn Gly Gln 
        755                 760                 765             


Asp Pro Ser Trp Leu Tyr Ile Glu Thr Ala Asp Gly Pro Gly Thr Ala 
    770                 775                 780                 


Gly Lys Arg Thr Gln Ser Arg Gln Lys Gln Ile Gln Thr Val Tyr Ala 
785                 790                 795                 800 


Asn Ala Ala Gln Glu Leu Ile Asp Ser Ala Val Arg Gly Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Ala Asp Lys Ala Ser Phe Thr Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Ala Pro Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser His Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Asn Ala Thr Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Asn Val Phe Thr Ser Thr Leu Phe Ala Ser Lys 
                885                 890                 895     


Met Ala Ala Thr Trp Arg Lys Trp His Glu Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Arg Asn Leu Met Leu Arg Pro Asp Glu Ile Asp Lys Phe 
        915                 920                 925             


Ala His Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Ala Ala Ala Gln Tyr Pro Asn Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu Leu Asp 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Tyr Ala Lys 
        995                 1000                 1005             


Leu Ala  Pro Phe Phe Thr Tyr  Gly Glu Phe Ala Lys  Val Asp Val 
    1010                 1015                 1020             


Lys Lys  Phe Arg Glu Phe Asn  Phe Ile Gly Ala Leu  Lys His Ala 
    1025                 1030                 1035             


Lys Lys  Asn Ile Val Ala Lys  Asp Thr Gly Glu Ile  Val Trp Asp 
    1040                 1045                 1050             


Lys Glu  Arg Asp Ile Gln Glu  Leu Asp Arg Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val Tyr Phe Glu Thr  Ala Asp Leu 
    1070                 1075                 1080             


Phe Lys  Gln Thr Ile Tyr Ala  Ala Lys Asp Ser Lys  Glu Arg Gly 
    1085                 1090                 1095             


Gly Ser  Lys Gln Leu Ile Pro  Lys Lys Arg Gly Tyr  Pro Thr Gln 
    1100                 1105                 1110             


Val Tyr  Gly Gly Ile His Gly  Lys Ile Arg Leu Thr  Trp Arg Trp 
    1115                 1120                 1125             


Leu Lys  Leu Leu Arg Arg Lys  Lys Met Ser Ile Glu  Leu Ser Glu 
    1130                 1135                 1140             


Leu Gln  Leu Leu Lys 
    1145             


<210>  745
<211>  1149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  745

Met Glu Arg Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile Gly 
1               5                   10                  15      


Trp Ala Val Ile Gly Asp Asp Phe Lys Ile Lys Arg Lys Lys Gly Lys 
            20                  25                  30          


Asn Leu Ile Gly Val Arg Leu Phe Lys Glu Gly Asp Thr Ala Ala Glu 
        35                  40                  45              


Arg Arg Gly Phe Arg Thr Gln Arg Arg Arg Leu Asn Arg Arg Lys Trp 
    50                  55                  60                  


Arg Leu Lys Leu Leu Glu Glu Ile Phe Asp Pro Tyr Met Ala Glu Val 
65                  70                  75                  80  


Asp Lys Tyr Phe Phe Ala Arg Leu Lys Glu Ser Asn Leu Ser Pro Lys 
                85                  90                  95      


Asp Ser Asn Lys Lys Tyr Leu Gly Ser Leu Leu Phe Pro Asp Ile Ser 
            100                 105                 110         


Asp Ser Asn Phe Tyr Asp Lys Tyr Pro Thr Ile Tyr His Leu Arg Arg 
        115                 120                 125             


Asp Leu Met Glu Lys Asp Lys Lys Phe Asp Leu Arg Glu Ile Tyr Leu 
    130                 135                 140                 


Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Glu Lys Val 
145                 150                 155                 160 


Pro Ala Lys Asn Tyr Lys Asn Ser Gly Ala Ser Ile Gly Phe Leu Leu 
                165                 170                 175     


Glu Glu Val Asn Asp Leu Tyr Gly Asn Ile Ile Gly Asn Glu Asp Val 
            180                 185                 190         


Ala Ile Leu Asp Asn Asp Lys Phe Glu Asp Val Glu Lys Ile Ile Leu 
        195                 200                 205             


Asn Asp Glu Ile Arg Asn Ile Asp Lys Gln Lys Asn Val Gly Arg Leu 
    210                 215                 220                 


Leu Val Lys Asp Lys Lys Glu Lys Asn Ile Val Thr Ala Phe Ser Lys 
225                 230                 235                 240 


Ala Ile Phe Gly Tyr Lys Phe Asn Leu Glu Asp Leu Leu Leu Ile Glu 
                245                 250                 255     


Ser Asp Glu Lys Asn Lys Leu Thr Phe Asn Asp Glu Asn Ile Asp Asp 
            260                 265                 270         


Ile Phe Asn Glu Leu Ser His Ser Leu Asn Asp Asn Gln Met Asp Leu 
        275                 280                 285             


Leu Thr Lys Thr Arg Glu Ile Tyr Phe Lys Phe Lys Leu Asn Met Ile 
    290                 295                 300                 


Val Pro Thr Gly Tyr Thr Ile Ser Glu Ser Met Ile Glu Lys Tyr Glu 
305                 310                 315                 320 


Met His Lys Ala His Leu Lys Met Tyr Lys Glu Phe Ile Asn Thr Leu 
                325                 330                 335     


Asn Ala Lys Asp Arg Lys Ile Leu Lys Asn Ala Tyr Ser Asp Tyr Ile 
            340                 345                 350         


Asn Asn Glu Lys Ala Lys Ala Ala Asn Ala Gln Glu Asn Phe Tyr Lys 
        355                 360                 365             


Thr Val Lys Lys Thr Ile Lys Glu Asn Asp Ser Asp Thr Ala Lys Lys 
    370                 375                 380                 


Ile Ile Gly Leu Ile Asp Glu Gly Asn Phe Met Pro Lys Gln Arg Thr 
385                 390                 395                 400 


Gly Glu Asn Gly Val Ile Pro His Gln Leu His Gln Ile Glu Leu Asp 
                405                 410                 415     


Arg Ile Ile Glu Asn Gln Ala Lys Tyr Tyr Pro Trp Leu Ala Glu Glu 
            420                 425                 430         


Asn Pro Val Glu Lys Asn Arg Lys Phe Ala Lys Tyr Lys Leu Asp Glu 
        435                 440                 445             


Leu Val Thr Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Asp Lys 
    450                 455                 460                 


Thr Glu Ser Asn Lys Asn Glu Lys Glu Thr Lys Phe Ala Trp Met Val 
465                 470                 475                 480 


Arg Lys Ala Lys Gly Thr Ile Thr Pro Trp Asn Phe Glu Asn Leu Val 
                485                 490                 495     


Asp Arg Thr Glu Ser Ala Asn Arg Phe Ile Lys Arg Met Thr Ser Lys 
            500                 505                 510         


Asp Thr Tyr Ile Ile Gly Glu Asp Val Leu Pro Ala Ser Ser Leu Leu 
        515                 520                 525             


Tyr Glu Lys Tyr Lys Val Leu Asn Glu Leu Asn Asn Ile Lys Val Asn 
    530                 535                 540                 


Lys Lys Lys Leu Asp Val Glu Gln Lys Gln His Val Tyr Leu Asp Leu 
545                 550                 555                 560 


Phe Thr Thr Arg Lys Asn Val Thr Lys Asp Asp Leu Ala Thr Ser Leu 
                565                 570                 575     


Asn Cys Asp Val Glu Ser Ile Thr Gly Leu Thr Asp Asn Lys Lys Phe 
            580                 585                 590         


Asn Ser Ser Leu Ser Ser Tyr Ile Asp Leu Lys Ala Ile Leu Gly Asn 
        595                 600                 605             


Ile Val Asp Asp Tyr Ser Lys Asn Glu Asp Leu Glu Lys Ile Ile Glu 
    610                 615                 620                 


Tyr Ser Thr Ile Phe Glu Asp Gly Asn Ile Tyr Lys Glu Lys Leu Ser 
625                 630                 635                 640 


Glu Ile Ser Trp Leu Thr Asp Glu Gln Ile Glu Lys Leu Ser Asn Ile 
                645                 650                 655     


His Phe Lys Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gln Ile 
            660                 665                 670         


Thr Asn Glu Asn Gly Glu Arg Ile Ile Asp Thr Leu Trp Asn Thr Ser 
        675                 680                 685             


Asn Asn Phe Ile Gln Val Ile Ser Asp Glu Ser Ile Gln Ala Lys Leu 
    690                 695                 700                 


Ala Glu Ile Asn Gly Glu Tyr Ala Asn Lys Tyr Asn Leu Glu Asp Ile 
705                 710                 715                 720 


Leu Asp Glu Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln 
                725                 730                 735     


Val Met Lys Val Val Glu Asp Ile Glu Lys Ala Met Lys Cys Glu Pro 
            740                 745                 750         


Thr Ser Ile Ala Ile Glu Phe Thr Arg Glu Lys Arg Lys Ser Lys Leu 
        755                 760                 765             


Thr Asn Thr Arg Tyr Lys Lys Ile Ser Glu Thr Tyr Glu Lys Ile Thr 
    770                 775                 780                 


Asp Glu Leu Ile Ser Glu Tyr Glu Leu Gly Lys Leu Gln Ser Glu Leu 
785                 790                 795                 800 


Asp Ser Lys Val Asn Asn Met Arg Asp Arg Tyr Tyr Leu Tyr Phe Met 
                805                 810                 815     


Gln Leu Gly Arg Asp Met Tyr Thr Gly Glu Lys Met Asn Ile Asp Glu 
            820                 825                 830         


Leu His Gln Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe Ile 
        835                 840                 845             


Lys Asp Asp Ser Leu Asn Asn Arg Val Leu Thr Ser Lys Ser Val Asn 
    850                 855                 860                 


Ile Lys Glu Lys Ser Asp Lys Thr Ala Ala Asp Leu Tyr Ala Ala Lys 
865                 870                 875                 880 


Met Gly Asp Phe Trp Arg Lys Leu Arg Lys Gln Gly Leu Met Thr Glu 
                885                 890                 895     


Gln Lys Tyr Lys Asn Leu Leu Thr Arg Thr Asp Ser Ile Asn Lys Tyr 
            900                 905                 910         


Thr Lys Gln Ser Phe Ile Lys Arg Gln Leu Val Glu Thr Ser Gln Val 
        915                 920                 925             


Val Lys Leu Ala Ala Asn Ile Leu Gln Asp Lys Tyr Arg Asn Thr Lys 
    930                 935                 940                 


Ile Ile Glu Ile Arg Ala Arg Leu Asn Ser Asp Leu Arg Lys Lys Tyr 
945                 950                 955                 960 


Glu Leu Ile Lys Asn Arg Glu Val Asn Asp Tyr His His Ala Ile Asp 
                965                 970                 975     


Gly Tyr Leu Thr Thr Phe Val Gly Gln Tyr Leu Tyr Lys Val Tyr Pro 
            980                 985                 990         


Lys Leu Arg Ser Tyr Phe Val Tyr  Asp Asp Phe Lys Lys  Leu Asp Ser 
        995                 1000                 1005             


Asn Tyr  Leu Lys His Met Asp  Lys Phe Asn Phe Ile  Trp Lys Leu 
    1010                 1015                 1020             


Glu Asp  Lys Lys Ala Glu Asp  Val Tyr Asp Lys Val  Asn Asp Glu 
    1025                 1030                 1035             


Phe Val  Leu Asn Val Pro Glu  Met Lys Glu Tyr Ile  Arg Lys Ile 
    1040                 1045                 1050             


Tyr Asn  Tyr Lys Tyr Met Leu  Val Ser Lys Glu Val  Thr Thr Lys 
    1055                 1060                 1065             


Asn Gly  Ala Phe Tyr Asp Gln  Thr Lys Tyr Asn Ala  Lys Thr Ile 
    1070                 1075                 1080             


Asn Leu  Ile Pro Ile Lys Lys  Asp Lys Pro Thr Asn  Ile Tyr Gly 
    1085                 1090                 1095             


Gly Tyr  Lys Gly Lys Val Ser  Ser Tyr Met Met Leu  Val Lys Ile 
    1100                 1105                 1110             


Gln Lys  Lys Lys Glu Val Ile  Tyr Lys Phe Val Gly  Val Pro Arg 
    1115                 1120                 1125             


Leu Trp  Thr Asp Glu Leu Asp  Arg Leu Ile Asp Thr  Asp Glu Lys 
    1130                 1135                 1140             


Lys Ala  Leu Leu Lys Lys 
    1145                 


<210>  746
<211>  1152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  746

Met Asn Ala Glu His Gly Lys Glu Gly Leu Leu Ile Met Glu Glu Asn 
1               5                   10                  15      


Phe Gln Tyr Arg Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
            20                  25                  30          


Ala Val Leu Gln Asn Asn Ser Gln Asp Glu Pro Val Arg Ile Thr Asp 
        35                  40                  45              


Leu Gly Val Arg Ile Phe Asp Val Ala Glu Asn Pro Lys Asn Gly Asp 
    50                  55                  60                  


Ala Leu Ala Ala Pro Arg Arg Asp Ala Arg Thr Thr Arg Arg Arg Leu 
65                  70                  75                  80  


Arg Arg Arg Arg His Arg Leu Glu Arg Ile Lys Phe Leu Leu Gln Glu 
                85                  90                  95      


Asn Gly Leu Ile Glu Met Asp Ser Phe Met Glu Arg Tyr Tyr Lys Gly 
            100                 105                 110         


Asn Leu Pro Asp Val Tyr Gln Leu Arg Tyr Glu Gly Leu Asp Arg Lys 
        115                 120                 125             


Leu Lys Asp Glu Glu Leu Ala Gln Val Leu Ile His Ile Ala Lys His 
    130                 135                 140                 


Arg Gly Phe Arg Ser Thr Arg Lys Ala Glu Thr Lys Glu Lys Glu Gly 
145                 150                 155                 160 


Gly Ala Val Leu Lys Ala Thr Thr Glu Asn Gln Lys Ile Met Gln Glu 
                165                 170                 175     


Lys Gly Tyr Arg Thr Val Gly Glu Met Leu Tyr Leu Asp Glu Ala Phe 
            180                 185                 190         


His Thr Glu Cys Leu Trp Asn Glu Lys Gly Tyr Val Leu Thr Pro Arg 
        195                 200                 205             


Asn Arg Pro Asp Asp Tyr Lys His Thr Ile Leu Arg Ser Met Leu Val 
    210                 215                 220                 


Glu Glu Val His Ala Ile Phe Ala Ala Gln Arg Ala His Gly Asn Gln 
225                 230                 235                 240 


Lys Ala Thr Glu Gly Leu Glu Glu Ala Tyr Val Glu Ile Met Thr Ser 
                245                 250                 255     


Gln Arg Ser Phe Asp Met Gly Pro Gly Leu Gln Pro Asp Gly Lys Pro 
            260                 265                 270         


Ser Pro Tyr Ala Met Glu Gly Phe Gly Asp Arg Val Gly Lys Cys Thr 
        275                 280                 285             


Phe Glu Lys Asp Glu Tyr Arg Ala Pro Lys Ala Thr Tyr Thr Ala Glu 
    290                 295                 300                 


Leu Phe Val Ala Leu Gln Lys Ile Asn His Thr Lys Leu Ile Asp Glu 
305                 310                 315                 320 


Phe Gly Thr Gly Arg Phe Phe Ser Glu Glu Glu Arg Lys Thr Ile Ile 
                325                 330                 335     


Gly Leu Leu Leu Ser Ser Lys Glu Leu Lys Tyr Gly Thr Ile Arg Lys 
            340                 345                 350         


Lys Leu Asn Ile Asp Pro Ser Leu Lys Phe Asn Ser Leu Asn Tyr Ser 
        355                 360                 365             


Ala Lys Lys Glu Gly Glu Thr Glu Glu Glu Arg Val Leu Asp Thr Glu 
    370                 375                 380                 


Lys Ala Lys Phe Ala Ser Met Phe Trp Thr Tyr Glu Tyr Ser Lys Cys 
385                 390                 395                 400 


Leu Lys Asp Arg Thr Glu Glu Met Pro Val Gly Glu Lys Ala Asp Leu 
                405                 410                 415     


Phe Asp Arg Ile Gly Glu Ile Leu Thr Ala Tyr Lys Asn Asp Asp Ser 
            420                 425                 430         


Arg Ser Ser Arg Leu Lys Glu Leu Gly Leu Ser Gly Glu Glu Ile Asp 
        435                 440                 445             


Gly Leu Leu Asp Leu Ser Pro Ala Lys Tyr Gln Arg Val Ser Leu Lys 
    450                 455                 460                 


Ala Met Arg Lys Met Gln Pro Tyr Leu Glu Asp Gly Leu Ile Tyr Asp 
465                 470                 475                 480 


Lys Ala Cys Glu Ala Ala Gly Tyr Asp Phe Arg Ala Leu Asn Asp Gly 
                485                 490                 495     


Asn Lys Lys His Leu Leu Lys Gly Glu Glu Ile Asn Ala Ile Val Asn 
            500                 505                 510         


Asp Ile Thr Asn Pro Val Val Lys Arg Ser Val Ser Gln Thr Ile Lys 
        515                 520                 525             


Val Ile Asn Ala Ile Ile Gln Lys Tyr Gly Ser Pro Gln Ala Val Asn 
    530                 535                 540                 


Ile Glu Leu Ala Arg Glu Met Ser Lys Asn Phe Gln Asp Arg Thr Asn 
545                 550                 555                 560 


Leu Glu Lys Glu Met Lys Lys Arg Gln Gln Glu Asn Glu Arg Ala Lys 
                565                 570                 575     


Gln Gln Ile Ile Glu Leu Gly Lys Gln Asn Pro Thr Gly Gln Asp Ile 
            580                 585                 590         


Leu Lys Tyr Arg Leu Trp Asn Asp Gln Gly Gly Tyr Cys Leu Tyr Ser 
        595                 600                 605             


Gly Lys Lys Ile Pro Leu Glu Glu Leu Phe Asp Gly Gly Tyr Asp Ile 
    610                 615                 620                 


Asp His Ile Leu Pro Tyr Ser Ile Thr Phe Asp Asp Ser Tyr Arg Asn 
625                 630                 635                 640 


Lys Val Leu Val Thr Ala Gln Glu Asn Arg Gln Lys Gly Asn Arg Thr 
                645                 650                 655     


Pro Tyr Glu Tyr Phe Gly Ala Asp Glu Lys Arg Trp Glu Asp Tyr Glu 
            660                 665                 670         


Ala Ser Val Arg Leu Leu Val Arg Asp Tyr Lys Lys Gln Gln Lys Leu 
        675                 680                 685             


Leu Lys Lys Asn Phe Thr Glu Glu Glu Arg Lys Glu Phe Lys Glu Arg 
    690                 695                 700                 


Asn Leu Asn Asp Thr Lys Tyr Ile Thr Arg Val Val Tyr Asn Met Ile 
705                 710                 715                 720 


Arg Gln Asn Leu Glu Leu Glu Pro Phe Asn His Pro Glu Lys Lys Lys 
                725                 730                 735     


Gln Val Trp Ala Val Asn Gly Ala Val Thr Ser Tyr Leu Arg Lys Arg 
            740                 745                 750         


Trp Gly Leu Met Gln Lys Asp Arg Ser Thr Asp Arg His His Ala Met 
        755                 760                 765             


Asp Ala Val Val Ile Ala Cys Cys Thr Asp Gly Met Ile His Lys Ile 
    770                 775                 780                 


Ser Arg Tyr Met Gln Gly Arg Glu Leu Ala Tyr Ser Arg Asn Phe Lys 
785                 790                 795                 800 


Phe Pro Asp Glu Glu Thr Gly Glu Ile Leu Asn Arg Asp Asn Phe Thr 
                805                 810                 815     


Arg Glu Gln Trp Asp Glu Lys Phe Gly Val Lys Val Pro Leu Pro Trp 
            820                 825                 830         


Asn Ser Phe Arg Asp Glu Leu Asp Ile Arg Leu Leu Asn Glu Asp Pro 
        835                 840                 845             


Lys Asn Phe Leu Leu Thr His Ala Asp Val Gln Arg Glu Leu Asp Tyr 
    850                 855                 860                 


Pro Gly Trp Met Tyr Gly Glu Glu Glu Ser Pro Ile Glu Glu Gly Arg 
865                 870                 875                 880 


Tyr Ile Asn Tyr Ile Arg Pro Leu Phe Val Ser Arg Met Pro Asn His 
                885                 890                 895     


Lys Val Thr Gly Ser Ala His Asp Ala Thr Ile Arg Ser Ala Arg Asp 
            900                 905                 910         


Tyr Glu Thr Arg Gly Val Val Ile Thr Lys Val Pro Leu Thr Asp Leu 
        915                 920                 925             


Lys Leu Asn Lys Asp Asn Glu Ile Glu Gly Tyr Tyr Asp Lys Asp Ser 
    930                 935                 940                 


Asp Arg Leu Leu Tyr Gln Ala Leu Val Arg Gln Leu Leu Leu His Gly 
945                 950                 955                 960 


Asn Asp Gly Lys Lys Ala Phe Ala Glu Asp Phe His Lys Pro Lys Ala 
                965                 970                 975     


Asp Gly Thr Glu Gly Pro Val Val Arg Lys Val Lys Ile Glu Lys Lys 
            980                 985                 990         


Gln Thr Ser Gly Val Met Val Arg  Gly Gly Thr Gly Ile  Ala Ala Asn 
        995                 1000                 1005             


Gly Glu  Met Val Arg Ile Asp  Val Phe Arg Glu Asn  Gly Lys Tyr 
    1010                 1015                 1020             


Tyr Phe  Val Pro Val Tyr Thr  Ala Asp Val Val Arg  Lys Val Leu 
    1025                 1030                 1035             


Pro Asn  Arg Ala Ala Thr His  Thr Lys Pro Tyr Ser  Glu Trp Arg 
    1040                 1045                 1050             


Val Met  Asp Asp Ala Asn Phe  Val Phe Ser Leu Tyr  Ser Arg Asp 
    1055                 1060                 1065             


Leu Ile  His Val Lys Ser Lys  Lys Asp Ile Lys Thr  Asn Leu Val 
    1070                 1075                 1080             


Asn Gly  Gly Leu Leu Leu Gln  Lys Glu Ile Phe Ala  Tyr Tyr Thr 
    1085                 1090                 1095             


Gly Ala  Asp Ile Ala Thr Ala  Ser Ile Ala Gly Phe  Ala Asn Asp 
    1100                 1105                 1110             


Ser Asn  Phe Lys Phe Arg Gly  Leu Gly Ile Gln Ser  Leu Glu Ile 
    1115                 1120                 1125             


Phe Glu  Lys Cys Gln Val Asp  Ile Leu Gly Asn Ile  Ser Val Val 
    1130                 1135                 1140             


Arg His  Glu Asn Arg Gln Glu  Phe His 
    1145                 1150         


<210>  747
<211>  1153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  747

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Leu Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ser Val Ile Arg Glu Asn Ser Glu Glu Gln Glu Leu Val Ala Met Gly 
            20                  25                  30          


Ser Arg Val Val Ser Leu Thr Ala Ala Glu Leu Ser Ser Phe Thr Gln 
        35                  40                  45              


Gly Asn Gly Val Ser Ile Asn Ser Gln Arg Thr Gln Lys Arg Thr Gln 
    50                  55                  60                  


Arg Lys Gly Tyr Asp Arg Tyr Gln Leu Arg Arg Thr Leu Leu Arg Asn 
65                  70                  75                  80  


Lys Leu Asp Thr Leu Gly Met Leu Pro Asp Asp Ser Leu Ser Tyr Leu 
                85                  90                  95      


Pro Lys Leu Gln Leu Trp Gly Leu Arg Ala Lys Ala Val Thr Gln Arg 
            100                 105                 110         


Ile Glu Leu Asn Glu Leu Gly Arg Val Leu Leu His Leu Asn Gln Lys 
        115                 120                 125             


Arg Gly Tyr Lys Ser Ile Lys Ser Asp Phe Ser Gly Asp Lys Lys Ile 
    130                 135                 140                 


Thr Asp Tyr Val Lys Thr Val Lys Thr Arg Tyr Asp Glu Leu Lys Glu 
145                 150                 155                 160 


Met Arg Leu Thr Ile Gly Glu Leu Phe Phe Arg Arg Leu Thr Glu Asn 
                165                 170                 175     


Ala Phe Phe Arg Cys Lys Glu Gln Val Tyr Pro Arg Gln Ala Tyr Val 
            180                 185                 190         


Glu Glu Phe Asp Cys Ile Met Asn Cys Gln Arg Lys Phe Tyr Pro Asp 
        195                 200                 205             


Ile Leu Thr Asp Glu Thr Ile Arg Cys Ile Arg Asp Glu Ile Ile Tyr 
    210                 215                 220                 


Tyr Gln Arg Pro Leu Lys Ser Cys Lys Tyr Leu Val Ser Arg Cys Glu 
225                 230                 235                 240 


Phe Glu Lys Arg Phe Tyr Leu Asn Ala Ala Gly Lys Lys Thr Glu Ala 
                245                 250                 255     


Gly Pro Lys Val Ser Pro Arg Thr Ser Pro Leu Phe Gln Val Cys Arg 
            260                 265                 270         


Leu Trp Glu Ser Ile Asn Asn Ile Val Val Lys Asp Arg Arg Asn Glu 
        275                 280                 285             


Ile Val Phe Ile Ser Ala Glu Gln Arg Ala Ala Leu Phe Asp Phe Leu 
    290                 295                 300                 


Asn Thr His Glu Lys Leu Lys Gly Ser Asp Leu Leu Lys Leu Leu Gly 
305                 310                 315                 320 


Leu Ser Lys Thr Tyr Gly Tyr Arg Leu Gly Glu Gln Phe Lys Thr Gly 
                325                 330                 335     


Ile Gln Gly Asn Lys Thr Arg Val Glu Ile Glu Arg Ala Leu Gly Asn 
            340                 345                 350         


Tyr Pro Asp Lys Lys Arg Leu Leu Gln Phe Asn Leu Gln Glu Glu Ser 
        355                 360                 365             


Ser Ser Met Val Asn Thr Glu Thr Gly Glu Ile Ile Pro Met Ile Ser 
    370                 375                 380                 


Leu Ser Phe Glu Gln Glu Pro Leu Tyr Arg Leu Trp His Val Leu Tyr 
385                 390                 395                 400 


Ser Ile Asp Asp Arg Glu Gln Leu Gln Ser Val Leu Arg Gln Lys Phe 
                405                 410                 415     


Gly Ile Asp Asp Asp Glu Val Leu Glu Arg Leu Ser Ala Ile Asp Leu 
            420                 425                 430         


Val Lys Ala Gly Phe Gly Asn Lys Ser Ser Lys Ala Ile Arg Arg Ile 
        435                 440                 445             


Leu Pro Phe Leu Gln Leu Gly Met Asn Tyr Ala Glu Ala Cys Glu Ala 
    450                 455                 460                 


Ala Gly Tyr Asn His Ser Asn Asn Tyr Thr Lys Ala Glu Asn Glu Ala 
465                 470                 475                 480 


Arg Ala Leu Leu Asp Arg Leu Pro Ala Ile Lys Lys Asn Glu Leu Arg 
                485                 490                 495     


Gln Pro Val Val Glu Lys Ile Leu Asn Gln Met Val Asn Val Val Asn 
            500                 505                 510         


Ala Leu Met Glu Lys Tyr Gly Arg Phe Asp Glu Ile Arg Val Glu Leu 
        515                 520                 525             


Ala Arg Glu Leu Lys Gln Ser Lys Glu Glu Arg Ser Asn Thr Tyr Lys 
    530                 535                 540                 


Ser Ile Asn Lys Asn Gln Arg Glu Asn Glu Gln Ile Ala Lys Arg Ile 
545                 550                 555                 560 


Val Glu Tyr Gly Val Pro Thr Arg Ser Arg Ile Gln Lys Tyr Lys Met 
                565                 570                 575     


Trp Glu Glu Ser Lys His Cys Cys Ile Tyr Cys Gly Gln Pro Val Asp 
            580                 585                 590         


Val Gly Asp Phe Leu Arg Gly Phe Asp Val Glu Val Glu His Ile Ile 
        595                 600                 605             


Pro Lys Ser Leu Tyr Phe Asp Asp Ser Phe Ala Asn Lys Val Cys Ser 
    610                 615                 620                 


Cys Arg Ser Cys Asn Lys Glu Lys Asn Asn Arg Thr Ala Tyr Asp Tyr 
625                 630                 635                 640 


Met Lys Ser Lys Gly Glu Lys Ala Leu Ser Asp Tyr Val Glu Arg Val 
                645                 650                 655     


Asn Thr Met Tyr Thr Asn Asn Gln Ile Ser Lys Thr Lys Trp Gln Asn 
            660                 665                 670         


Leu Leu Thr Pro Val Asp Lys Ile Ser Ile Asp Phe Ile Asp Arg Gln 
        675                 680                 685             


Leu Arg Glu Ser Gln Tyr Ile Ala Arg Lys Ala Lys Glu Ile Leu Thr 
    690                 695                 700                 


Ser Ile Cys Tyr Asn Val Thr Ala Thr Ser Gly Ser Val Thr Ser Phe 
705                 710                 715                 720 


Leu Arg His Val Trp Gly Trp Asp Thr Val Leu His Asp Leu Asn Phe 
                725                 730                 735     


Asp Arg Tyr Lys Lys Val Gly Leu Thr Glu Val Ile Glu Val Asn His 
            740                 745                 750         


Arg Gly Ser Val Ile Arg Arg Glu Gln Ile Lys Asp Trp Ser Lys Arg 
        755                 760                 765             


Phe Asp His Arg His His Ala Ile Asp Ala Leu Thr Ile Ala Cys Thr 
    770                 775                 780                 


Lys Gln Ala Tyr Ile Gln Arg Leu Asn Asn Leu Arg Ala Glu Glu Gly 
785                 790                 795                 800 


Pro Asp Phe Asn Lys Met Ser Leu Glu Arg Tyr Ile Gln Ser Gln Pro 
                805                 810                 815     


His Phe Ser Val Ala Gln Val Arg Glu Ala Val Asp Arg Ile Leu Val 
            820                 825                 830         


Ser Phe Arg Ala Gly Lys Arg Ala Val Thr Pro Gly Lys Arg Tyr Ile 
        835                 840                 845             


Arg Lys Asn Arg Lys Arg Ile Ser Val Gln Ser Val Leu Ile Pro Arg 
    850                 855                 860                 


Gly Ala Leu Ser Glu Glu Ser Val Tyr Gly Val Ile His Val Trp Glu 
865                 870                 875                 880 


Lys Asp Glu Gln Gly His Val Ile Gln Lys Gln Arg Ala Val Met Lys 
                885                 890                 895     


Tyr Pro Ile Thr Ser Ile Asn Arg Glu Met Leu Asp Lys Glu Lys Val 
            900                 905                 910         


Val Asp Lys Arg Ile His Arg Ile Leu Ser Gly Arg Leu Ala Gln Tyr 
        915                 920                 925             


Asn Asp Asn Pro Lys Glu Ala Phe Ala Lys Pro Val Tyr Ile Asp Lys 
    930                 935                 940                 


Glu Cys Arg Ile Pro Ile Arg Thr Val Arg Cys Phe Ala Lys Pro Ala 
945                 950                 955                 960 


Ile Asn Thr Leu Val Pro Leu Lys Lys Asp Asp Lys Gly Asn Pro Val 
                965                 970                 975     


Ala Trp Val Asn Pro Gly Asn Asn His His Val Ala Ile Tyr Arg Asp 
            980                 985                 990         


Glu Asp Gly Lys Tyr Lys Glu Arg  Thr Val Thr Phe Trp  Glu Ala Val 
        995                 1000                 1005             


Asp Arg  Cys Arg Val Gly Ile  Pro Ala Ile Val Thr  Gln Pro Asp 
    1010                 1015                 1020             


Thr Ile  Trp Asp Asn Ile Leu  Gln Arg Asn Asp Ile  Ser Glu Asn 
    1025                 1030                 1035             


Val Leu  Glu Ser Leu Pro Asp  Val Lys Trp Gln Phe  Val Leu Ser 
    1040                 1045                 1050             


Leu Gln  Gln Asn Glu Met Phe  Ile Leu Gly Met Asn  Glu Glu Asp 
    1055                 1060                 1065             


Tyr Arg  Tyr Ala Met Asp Gln  Gln Asp Tyr Ala Leu  Leu Asn Lys 
    1070                 1075                 1080             


Tyr Leu  Tyr Arg Val Gln Lys  Leu Ser Lys Ser Asp  Tyr Ser Phe 
    1085                 1090                 1095             


Arg Tyr  His Thr Glu Thr Ser  Val Glu Asp Lys Tyr  Asp Gly Lys 
    1100                 1105                 1110             


Pro Asn  Leu Lys Leu Ser Met  Gln Met Gly Lys Leu  Lys Arg Val 
    1115                 1120                 1125             


Ser Ile  Lys Ser Leu Leu Gly  Leu Asn Pro His Lys  Val His Ile 
    1130                 1135                 1140             


Ser Val  Leu Gly Glu Ile Lys  Glu Ile Ser 
    1145                 1150             


<210>  748
<211>  1157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  748

Met Asn Tyr Tyr Asn Gly Phe Ile Ile Thr Glu Asn Ala Met Thr Ser 
1               5                   10                  15      


Lys Gln Glu Lys His Gly Ala Leu Thr Phe Gly Leu Asp Ile Gly Ile 
            20                  25                  30          


Ala Ser Val Gly Trp Ala Val Leu Gly Glu Ser Arg Ile Val Asp Leu 
        35                  40                  45              


Gly Val Arg Cys Phe Asp Lys Ala Glu Thr Ala Lys Glu Gly Glu Ser 
    50                  55                  60                  


Leu Asn Leu Thr Arg Arg Met Ala Arg Leu Leu Arg Arg Arg Leu Arg 
65                  70                  75                  80  


Arg Arg Ala Trp Arg Leu Thr Lys Leu Ala Arg Leu Leu Lys Arg Glu 
                85                  90                  95      


Gly Leu Ile Ala Asp Ala Asn Leu Phe Lys His Lys Ile Glu Lys Asn 
            100                 105                 110         


Thr Pro Val Pro Lys Ser Ser Trp Gln Leu Arg Val Asp Gly Leu Asp 
        115                 120                 125             


Arg Gln Leu Asn Ala Glu Glu Trp Ala Arg Val Ile Tyr His Leu Cys 
    130                 135                 140                 


Lys His Arg Gly Phe His Trp Ile Ser Arg Ala Glu Glu Lys Ala Ala 
145                 150                 155                 160 


Glu Gly Asp Ser Lys Gly Glu Gly Gly Lys Val Lys Gln Gly Leu Ala 
                165                 170                 175     


Gly Thr Lys Lys Leu Met Gln Glu Lys Ser Tyr Arg Thr Ala Ala Glu 
            180                 185                 190         


Met Val Leu Ala Glu Phe Pro Asp Ala Gln Arg Asn Lys Gln Gly Asp 
        195                 200                 205             


Tyr Ser Lys Ala Leu Ser Arg Ile Leu Leu Gly Asp Glu Leu Lys Ala 
    210                 215                 220                 


Leu Phe Glu His Gln Arg Lys Leu Gly Asn Pro Val Ala Thr Glu Lys 
225                 230                 235                 240 


Leu Glu Ala Ala Ile Leu Gly Asn Gly Asp Lys Lys Cys Gly Leu Phe 
                245                 250                 255     


Trp Ala Gln Asn Pro Ala Leu Ala Gly Asn Lys Leu Leu Glu Met Leu 
            260                 265                 270         


Gly Arg Cys Thr Phe Glu Lys Thr Gly Gly Asp Asp Gly Lys Gly Glu 
        275                 280                 285             


Tyr Arg Ala Pro Lys Ala Ser Phe Ser Ala Glu Arg His Val Trp Leu 
    290                 295                 300                 


Thr His Leu Asn Asn Leu Arg Ile Val Val Asp Gly Thr Thr Arg Pro 
305                 310                 315                 320 


Leu Asn Glu Ala Glu Arg Arg Val Ala Leu Pro Met Pro Tyr Gln Gln 
                325                 330                 335     


Ala Gly Asp Leu Ser Tyr Lys Gln Leu Gly Gly Ala Leu Ile Lys Glu 
            340                 345                 350         


Gly Phe Leu Gln Lys Asp Gly Phe Lys Phe Ala Gly Leu Pro Tyr Pro 
        355                 360                 365             


Thr Lys Gln Ala Ser Glu Glu Lys Ala Lys Asp Pro Glu Thr Ser Val 
    370                 375                 380                 


Leu Cys Lys Leu Pro Ala Trp His Thr Leu Arg Lys Thr Leu Glu Lys 
385                 390                 395                 400 


His Gly Leu Glu Ser Glu Trp Gln Lys Leu Ser Thr Asp Ala Leu Asp 
                405                 410                 415     


Gly Lys Pro Gln Leu Leu Asp Asn Ile Ala Trp Val Leu Ser Val Tyr 
            420                 425                 430         


Lys Asp Asp Asp Glu Val Lys Thr Glu Leu Gly Lys Leu Asn Leu Pro 
        435                 440                 445             


Asn Thr Glu Lys Met Ile Asp Ala Leu Leu Asp Ile Arg Phe Asp Lys 
    450                 455                 460                 


Phe Ser Asn Leu Ser Leu Lys Ala Leu Ser Lys Ile Val Pro Phe Met 
465                 470                 475                 480 


Glu Gln Gly Gln Arg Tyr Asp Glu Ala Val Ala Asn Ile Pro Glu Tyr 
                485                 490                 495     


Gly His His Ser Gln Leu His Lys Val Gly Ala Gly Glu His Lys Tyr 
            500                 505                 510         


Leu Pro Pro Phe Tyr Lys Glu Arg Asp Lys Thr Gly Arg Met Val Phe 
        515                 520                 525             


Asn Asp Asp Met Asp Ile Pro Arg Asn Pro Val Val Leu Arg Ala Leu 
    530                 535                 540                 


Asn Gln Ala Arg Lys Val Val Asn Ala Leu Ile Lys Glu Tyr Gly Ser 
545                 550                 555                 560 


Pro His Glu Val His Ile Glu Met Ala Arg Asp Leu Ser Arg Pro Leu 
                565                 570                 575     


Asp Glu Arg Arg Asp Ile Glu Lys Leu Gln Lys Glu Phe Gly Glu Arg 
            580                 585                 590         


Asn Lys Lys Asp Lys Glu Leu Phe Ala Gln Asp Phe Asn Ile Val Gly 
        595                 600                 605             


Thr Val Lys Gly Lys Gln Phe Glu Lys Tyr Gln Leu Tyr Arg Glu Gln 
    610                 615                 620                 


Gln Gly Lys Cys Ile Tyr Pro Ser Met Asp Glu Lys Gln Gly Ile Cys 
625                 630                 635                 640 


Cys Tyr Ser Leu Lys Pro Leu Asp Leu His Arg Val Leu Tyr Glu Glu 
                645                 650                 655     


Gly Tyr Ala Glu Ile Asp His Ala Leu Pro Tyr Ser Arg Ser Phe Asp 
            660                 665                 670         


Asp Ser Lys Asn Asn Arg Val Leu Val Leu Ala Lys Glu Asn Arg Asp 
        675                 680                 685             


Lys Gly Asn Arg Thr Pro Phe Glu Tyr Leu Gly Asp Lys Asp Asn Ser 
    690                 695                 700                 


Glu Pro Trp Arg Gln Phe Val Ala Phe Val Glu Gly Asn Lys Ala Tyr 
705                 710                 715                 720 


Arg Leu Ala Lys Arg Ser Arg Leu Leu Arg Lys Asp Phe Gly Asp Lys 
                725                 730                 735     


Glu Ala Lys Glu Phe Arg Glu Arg Asn Leu Asn Asp Thr Arg Tyr Ile 
            740                 745                 750         


Cys Lys Phe Phe Lys Asn Tyr Val Glu Gln Tyr Leu Gln Leu His Glu 
        755                 760                 765             


Asp Ser Glu Ala Lys Arg Cys Val Val Leu Ser Gly Gln Met Thr Ser 
    770                 775                 780                 


Phe Leu Arg Ala Arg Trp Gly Leu Val Lys Val Arg Gly Asp Ser Asp 
785                 790                 795                 800 


Arg His His Ala Leu Asp Ala Ala Val Val Ala Ala Cys Ser His Gly 
                805                 810                 815     


Met Val Lys Arg Leu Ser Asp Tyr Ser Arg Arg Lys Glu Leu Glu Gln 
            820                 825                 830         


Val Arg Glu Gly Phe Val Asp Val Asp Thr Gly Glu Ile Val Asn Pro 
        835                 840                 845             


Ala Met Phe Gln Gln Val Gln Glu His Phe Pro Asn Pro Trp Pro His 
    850                 855                 860                 


Phe Arg Asp Glu Val Lys Leu Arg Leu Ser Ile Asp Asp Pro Thr Leu 
865                 870                 875                 880 


Leu Arg Ser Glu Met Glu Lys Phe Gly Thr Tyr Thr Pro Glu Ser Leu 
                885                 890                 895     


Glu Ala Leu Arg Pro Leu Phe Val Ser Arg Ala Pro Gln Arg Arg Asn 
            900                 905                 910         


Ser Gly Ala Ala His Lys Asp Thr Ile Tyr Ala Gln Pro Lys Arg Leu 
        915                 920                 925             


Val Ala Gln Gly Gly Val Thr Gln Lys Val Ala Leu Ser Ser Leu Thr 
    930                 935                 940                 


Leu Lys Asp Leu Asp Lys Leu Ile Asp Pro His Arg Asn Glu Arg Leu 
945                 950                 955                 960 


Tyr Ala Ala Ile Arg Ala Arg Leu Glu Gln His Gly Gly Lys Gly Asp 
                965                 970                 975     


Lys Ala Phe Pro Ala Ser Asn Pro Leu Arg Lys Pro Asp Ser Glu Gly 
            980                 985                 990         


Asn Pro Thr Gly Pro Ile Val Arg  Gly Val Thr Met Val  Ile Asp Lys 
        995                 1000                 1005             


Leu Ser  Gly Ile Pro Ile Arg  Gly Gly Ile Ala Lys  Asn Asp Ser 
    1010                 1015                 1020             


Met Val  Arg Val Asp Val Phe  Thr Lys Ala Asn Lys  Phe His Leu 
    1025                 1030                 1035             


Val Pro  Val Tyr Val His His  Ser Val Leu Gly Leu  Pro Asn Gln 
    1040                 1045                 1050             


Ala Ile  Val Gly Gly Lys Glu  Glu Ser Glu Trp Thr  Gln Ile Asp 
    1055                 1060                 1065             


Asp Ser  Phe Asp Trp His Phe  Ser Ile Tyr Pro Asn  Asp Leu Ile 
    1070                 1075                 1080             


Arg Val  Thr Leu Lys Gln Glu  Gly Arg Phe Gly Tyr  Tyr Ala Gly 
    1085                 1090                 1095             


Cys Asp  Arg Ser Thr Ala Ala  Ile His Leu Trp Ala  His Asp Arg 
    1100                 1105                 1110             


Asn Gln  Gln Val Gly Lys Asp  Gly Leu Ile Arg Ser  Ile Gly Val 
    1115                 1120                 1125             


Lys Thr  Ala Leu Ser Ile Glu  Lys Phe Asn Val Asp  Val Leu Gly 
    1130                 1135                 1140             


Asn Arg  Tyr Pro Ala Pro Pro  Glu Lys Arg Arg Asp  Leu Ala 
    1145                 1150                 1155         


<210>  749
<211>  1161
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  749

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Ser Ala Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Glu Glu Thr Glu Gln Met Gln Tyr Asn Ile Val Ser Leu 
            20                  25                  30          


Gly Ser Arg Ile Ile Pro Tyr Asn Gly Thr Glu Gly Gln Glu Phe Glu 
        35                  40                  45              


Lys Gly Thr Gly Glu Ser Arg Asn Ser Leu Arg Thr Lys Ala Arg Thr 
    50                  55                  60                  


Ala Arg Lys Gly Tyr Asp Arg Tyr Gln Leu Arg Arg Lys Asn Leu Glu 
65                  70                  75                  80  


Lys Ile Leu Leu Gln Asn Gly Met Tyr Pro Asn Glu Gln Val Arg Ala 
                85                  90                  95      


Leu Gln Lys Leu Glu Leu Trp Lys Leu Arg Ala Asp Ala Pro Asn Val 
            100                 105                 110         


Gln Ile Ser Leu Pro Glu Leu Gly Arg Leu Leu Phe Trp Leu Asn Gln 
        115                 120                 125             


Lys Arg Gly Tyr Lys Ser Ser Arg Ser Asp Ala Asn Leu Asp Lys Lys 
    130                 135                 140                 


Asp Thr Glu Tyr Val Ala Thr Val Lys Ser Arg His Gln Lys Ile Lys 
145                 150                 155                 160 


Asp Ala Gly Gln Thr Val Gly Gln Tyr Phe Tyr Gly Gln Ile Leu Glu 
                165                 170                 175     


Asn Glu Tyr His Arg Ile Lys Glu Gln Val Phe Pro Arg Glu Ala Tyr 
            180                 185                 190         


Ile Glu Glu Phe Asp Ala Ile Val Ala Arg Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Thr Ile Leu Lys Gln Glu Leu Ile Asp Lys Ile Arg Asn Glu Val Ile 
    210                 215                 220                 


Tyr Phe Gln Arg Gly Leu Lys Ser Gln Lys Gly Leu Val Ser Val Cys 
225                 230                 235                 240 


Glu Phe Glu Gly Arg Glu Phe Asn Gly His Phe Gly Gly Pro Lys Val 
                245                 250                 255     


Ala Pro Lys Ser Ser Pro Ile Phe Gln Leu Gly Lys Ile Trp Glu Asn 
            260                 265                 270         


Ile Asn Asn Ile Lys Ile Ala Asp Ile Glu Leu Thr Ala Glu Gln Lys 
        275                 280                 285             


Leu Val Phe Phe Asn Tyr Leu Asn Cys His Glu Arg Ile Thr Glu Ala 
    290                 295                 300                 


Lys Ile Arg Glu Leu Leu Lys Leu Ser Lys Asp Ser Arg Leu Asn Gly 
305                 310                 315                 320 


Gln Leu Lys Asn Gly Leu Thr Gly Asn Lys Thr Tyr Ser Gln Ile Ala 
                325                 330                 335     


Ser Cys Phe Gly Asn Lys Ile Gly Glu Tyr Glu Arg Leu Phe Lys Phe 
            340                 345                 350         


Glu Leu Glu Val Ile Glu Ser Glu Lys Leu Glu Tyr Thr Tyr His Pro 
        355                 360                 365             


Lys Ser His Ile Pro Val Gly Glu Lys Arg Arg Lys Tyr Ile Ser Glu 
    370                 375                 380                 


Asn Val Glu Lys Glu Pro Tyr Tyr Arg Leu Trp His Thr Ile Tyr Ser 
385                 390                 395                 400 


Ile Lys Asp Lys Asp Glu Cys Arg Ala Ala Leu Ile Lys Asn Phe Gly 
                405                 410                 415     


Ile Glu Glu Glu Ile Ala Glu Lys Leu Ala Ser Leu Asp Phe Ser Ala 
            420                 425                 430         


Gly Gly Phe Gly Asn Lys Cys Ala Lys Val Ile Arg Lys Ile Leu Pro 
        435                 440                 445             


Tyr Leu Met Glu Gly Asp Lys Tyr Asn Val Ala Met Asp Tyr Ala Gly 
    450                 455                 460                 


Tyr Asn His Ser Phe Ser Gln Thr Lys Asp Glu Asn Leu Gln Arg Val 
465                 470                 475                 480 


Leu Leu Asp Ser Ile Asn His Leu Pro Lys Asn Ser Leu Arg Gln Pro 
                485                 490                 495     


Ile Val Glu Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Glu Val 
            500                 505                 510         


Ile Lys Gln Trp Gly Arg Pro Asp Glu Ile Arg Val Glu Leu Ala Arg 
        515                 520                 525             


Lys Leu Lys Gln Ser Lys Thr Glu Arg Asn Asp Asp Asp Asn Phe Met 
    530                 535                 540                 


Arg Lys Arg Glu Arg Glu Asn Glu Ile Ile Lys Ala Asp Leu Ala Lys 
545                 550                 555                 560 


Tyr Gly Leu Lys Ala Thr Arg Asn Asn Val Ile Lys Trp Arg Leu Tyr 
                565                 570                 575     


His Glu Ile Asp Asp Lys Asp Lys Lys Leu Asn Ala Arg Cys Ile Tyr 
            580                 585                 590         


Cys Gly Gln Pro Ile Ser Leu Thr Glu Ala Ile Leu Gly Asn Asn Val 
        595                 600                 605             


Asp Val Glu His Ile Ile Pro Lys Asp Lys Leu Phe Asp Asp Ser Gln 
    610                 615                 620                 


Ser Asn Lys Thr Leu Ala His Arg Ser Cys Asn Ser Gly Lys Gly Asn 
625                 630                 635                 640 


Lys Thr Ala Tyr Asp Phe Met Lys Asp Lys Pro Gln Phe Glu Glu Tyr 
                645                 650                 655     


Val Ala Arg Val Glu Glu Leu Tyr Lys Lys His Leu Ile Ser Leu Thr 
            660                 665                 670         


Lys Lys Arg Lys Phe Leu Met Ser Glu Asp Lys Ile Pro Ser Asp Phe 
        675                 680                 685             


Ile Asp Arg Gln Leu Arg Glu Ser Gln Tyr Ile Ala Arg Lys Ser Arg 
    690                 695                 700                 


Glu Ile Leu Gln Thr Val Cys Arg Asn Val Trp Ser Thr Asp Gly Gly 
705                 710                 715                 720 


Val Thr Ala His Leu Arg Lys Leu Trp Gly Trp Asp Asp Val Thr Met 
                725                 730                 735     


Asn Leu Gln Met Pro Lys Tyr Lys Gly Ile Gly Met Thr Glu Ile Val 
            740                 745                 750         


Glu Trp Glu Ser Asn His Gly Lys Asn Gln His Thr Gln Glu Val Ile 
        755                 760                 765             


Lys Asp Trp Ser Lys Arg Asp Asp His Arg His His Ala Ile Asp Ala 
    770                 775                 780                 


Leu Val Val Ala Cys Thr Arg Gln Gly Tyr Ile Gln Arg Ile Asn Thr 
785                 790                 795                 800 


Leu Asn Ser Ser Glu Thr Lys Asp Glu Met His Arg Lys Val Ala Gly 
                805                 810                 815     


Ser Leu Val Glu Phe Asn Gln Lys His Thr Leu Leu Asn Lys Tyr Leu 
            820                 825                 830         


Ile Thr Glu Lys Pro Phe Thr Thr Ser Glu Val Glu Asp Val Val Ser 
        835                 840                 845             


Lys Ile Leu Val Ser Phe Lys Ala Gly Lys Lys Ala Ala Thr Pro Gly 
    850                 855                 860                 


Lys Arg Lys Val Gly Lys Arg Gly Asn Lys Gln Val Val Gln Asp Gly 
865                 870                 875                 880 


Ile Ile Ile Pro Arg Gly Ala Leu His Ala Asp Ser Val Ser Gly Ser 
                885                 890                 895     


Ile Lys Gln Tyr Asp Pro Thr Gly Asn Phe Glu Asn His Asn Val Ile 
            900                 905                 910         


Lys Tyr Lys Val Gly Val Gly Lys Gly Tyr Leu Phe Asp Gly Lys Asp 
        915                 920                 925             


Asp Lys Lys Lys Ile Asp Lys Thr Ile Asp Cys Ile Val Asp Lys Lys 
    930                 935                 940                 


Ile Arg Asp Leu Ile Lys Gln Arg Ala Glu Glu Ser Asn Tyr Ser Leu 
945                 950                 955                 960 


Lys Lys Met Phe Glu Thr Pro Ile Leu Phe Asn Gln Arg Ala Val Phe 
                965                 970                 975     


Ser Val Arg Cys Lys Thr Gly Asn Lys Ala Val Val Pro Ile Arg Asn 
            980                 985                 990         


Gly Tyr Val Val Pro Asp Asn Asn  His His Thr Ala Ile  Tyr Lys Tyr 
        995                 1000                 1005             


Asp Gly  Gly Leu Lys Glu Gln  Cys Val Ser Leu Met  Gln Ala Ile 
    1010                 1015                 1020             


Glu Arg  Lys Arg Tyr Asn Leu  Pro Ile Ile Ile Lys  Asn Pro Lys 
    1025                 1030                 1035             


Glu Ile  Tyr Asp Lys Ile Thr  Glu Ile Ala Asn Ile  Asn Gln Leu 
    1040                 1045                 1050             


Leu Phe  Asp Ser Leu Pro Lys  Leu Asp Trp Glu Phe  Val Thr Thr 
    1055                 1060                 1065             


Leu Gln  Lys Asn Asp Met Phe  Ile Leu Gly Met Asp  Tyr Ser Ala 
    1070                 1075                 1080             


Thr Glu  Glu Ala Leu Ala Asp  Gly Asn Tyr Arg Leu  Leu Ser Glu 
    1085                 1090                 1095             


Tyr Leu  Tyr Lys Val Gln Asn  Ile Ser Asp Gly Ile  Tyr Arg Leu 
    1100                 1105                 1110             


Cys His  His Thr Ile Thr Lys  Phe Asp Leu Ser Lys  Ala Asn Lys 
    1115                 1120                 1125             


Pro Asp  Lys Arg Phe Tyr Asn  Ile Gln Ser Leu Lys  Ala Trp Asn 
    1130                 1135                 1140             


Thr Leu  Asn Pro Gln Lys Val  Lys Ile Asn Ile Leu  Gly Gln Ile 
    1145                 1150                 1155             


Glu Arg  Gln 
    1160     


<210>  750
<211>  1166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  750

Met His Val Glu Ile Asp Phe Pro His Phe Ser Arg Gly Asp Ser His 
1               5                   10                  15      


Leu Ala Met Asn Lys Asn Glu Ile Leu Arg Gly Ser Ser Val Leu Tyr 
            20                  25                  30          


Arg Leu Gly Leu Asp Leu Gly Ser Asn Ser Leu Gly Trp Phe Val Thr 
        35                  40                  45              


His Leu Glu Lys Arg Gly Asp Arg His Glu Pro Val Ala Leu Gly Pro 
    50                  55                  60                  


Gly Gly Val Arg Ile Phe Pro Asp Gly Arg Asp Pro Gln Ser Gly Thr 
65                  70                  75                  80  


Ser Asn Ala Val Asp Arg Arg Met Ala Arg Gly Ala Arg Lys Arg Arg 
                85                  90                  95      


Asp Arg Phe Val Glu Arg Arg Lys Glu Leu Ile Ala Ala Leu Ile Lys 
            100                 105                 110         


Tyr Asn Leu Leu Pro Asp Asp Ala Arg Glu Arg Arg Ala Leu Glu Val 
        115                 120                 125             


Leu Asp Pro Tyr Ala Leu Arg Lys Thr Ala Leu Thr Asp Thr Leu Pro 
    130                 135                 140                 


Ala His His Val Gly Arg Ala Leu Phe His Leu Asn Gln Arg Arg Gly 
145                 150                 155                 160 


Phe Gln Ser Asn Arg Lys Thr Asp Ser Lys Gln Ser Glu Asp Gly Ala 
                165                 170                 175     


Ile Lys Gln Ala Ala Ser Arg Leu Ala Thr Asp Lys Gly Asn Glu Thr 
            180                 185                 190         


Leu Gly Val Phe Phe Ala Asp Met His Leu Arg Lys Ser Tyr Glu Asp 
        195                 200                 205             


Arg Gln Thr Ala Ile Arg Ala Glu Leu Val Arg Leu Gly Lys Asp His 
    210                 215                 220                 


Leu Thr Gly Asn Ala Arg Lys Lys Ile Trp Ala Lys Val Arg Lys Arg 
225                 230                 235                 240 


Leu Phe Gly Asp Glu Val Leu Pro Arg Ala Asp Ala Pro His Gly Val 
                245                 250                 255     


Arg Ala Arg Ala Thr Ile Thr Gly Thr Lys Ala Ser Tyr Asp Tyr Tyr 
            260                 265                 270         


Pro Thr Arg Asp Met Leu Arg Asp Glu Phe Asn Ala Ile Trp Ala Gly 
        275                 280                 285             


Gln Ser Ala His His Ala Thr Ile Thr Asp Glu Ala Arg Thr Glu Ile 
    290                 295                 300                 


Glu His Ile Ile Phe Tyr Gln Arg Pro Leu Lys Pro Ala Ile Val Gly 
305                 310                 315                 320 


Lys Cys Thr Leu Asp Pro Ala Thr Arg Pro Phe Lys Glu Asp Pro Glu 
                325                 330                 335     


Gly Tyr Arg Ala Pro Trp Ser His Pro Leu Ala Gln Arg Phe Arg Ile 
            340                 345                 350         


Leu Ser Glu Ala Arg Asn Leu Glu Ile Arg Asp Thr Gly Lys Gly Ser 
        355                 360                 365             


Arg Arg Leu Thr Lys Glu Gln Ser Asp Leu Val Val Ala Ala Leu Leu 
    370                 375                 380                 


Ala Asn Arg Glu Val Lys Phe Asp Lys Leu Arg Thr Leu Leu Lys Leu 
385                 390                 395                 400 


Pro Ala Glu Ala Arg Phe Asn Leu Glu Ser Asp Arg Arg Ala Ala Leu 
                405                 410                 415     


Asp Gly Asp Gln Thr Ala Ala Arg Leu Ser Asp Lys Lys Gly Phe Asn 
            420                 425                 430         


Lys Ala Trp Arg Gly Phe Pro Pro Glu Arg Gln Ile Ala Ile Val Ala 
        435                 440                 445             


Arg Leu Glu Glu Thr Glu Asp Glu Asn Glu Leu Ile Ala Trp Leu Glu 
    450                 455                 460                 


Lys Glu Cys Ala Leu Asp Gly Ala Ala Ala Ala Arg Val Ala Asn Thr 
465                 470                 475                 480 


Thr Leu Pro Asp Gly His Cys Arg Leu Gly Leu Arg Ala Ile Lys Lys 
                485                 490                 495     


Ile Val Pro Ile Met Gln Asp Gly Leu Asp Glu Asp Gly Val Ala Gly 
            500                 505                 510         


Ala Gly Tyr His Ile Ala Ala Lys Arg Ala Gly Tyr Asp His Ala Lys 
        515                 520                 525             


Leu Pro Thr Gly Glu Gln Leu Gly Arg Leu Pro Tyr Tyr Gly Gln Trp 
    530                 535                 540                 


Leu Gln Asp Ala Val Val Gly Ser Gly Asp Ala Arg Asp Gln Lys Glu 
545                 550                 555                 560 


Lys Gln Tyr Gly Gln Phe Pro Asn Pro Thr Val His Ile Gly Leu Gly 
                565                 570                 575     


Gln Leu Arg Arg Val Val Asn Asp Leu Ile Asp Lys Tyr Gly Pro Pro 
            580                 585                 590         


Thr Glu Ile Ser Ile Glu Phe Thr Arg Ala Leu Lys Leu Ser Glu Gln 
        595                 600                 605             


Gln Lys Ala Glu Arg Gln Arg Glu Gln Arg Arg Asn Gln Asp Lys Asn 
    610                 615                 620                 


Lys Ala Arg Ala Glu Glu Leu Ala Lys Phe Gly Arg Pro Ala Asn Pro 
625                 630                 635                 640 


Arg Asn Leu Leu Lys Met Arg Leu Trp Glu Glu Leu Ala His Asp Pro 
                645                 650                 655     


Leu Asp Arg Lys Cys Val Tyr Thr Gly Glu Gln Ile Ser Ile Glu Arg 
            660                 665                 670         


Leu Leu Ser Asp Glu Val Asp Ile Asp His Ile Leu Pro Val Ala Met 
        675                 680                 685             


Thr Leu Asp Asp Ser Pro Ala Asn Lys Ile Ile Cys Met Arg Tyr Ala 
    690                 695                 700                 


Asn Arg His Lys Arg Lys Gln Thr Pro Ser Glu Ala Phe Gly Ser Ser 
705                 710                 715                 720 


Pro Thr Leu Gln Gly His Arg Tyr Asn Trp Asp Asp Ile Ala Ala Arg 
                725                 730                 735     


Ala Thr Gly Leu Pro Arg Asn Lys Arg Trp Arg Phe Asp Ala Asn Ala 
            740                 745                 750         


Arg Glu Glu Phe Asp Lys Arg Gly Gly Phe Leu Ala Arg Gln Leu Asn 
        755                 760                 765             


Glu Thr Gly Trp Leu Ala Arg Leu Ala Lys Gln Tyr Leu Gly Ala Val 
    770                 775                 780                 


Thr Asp Pro Asn Gln Ile Trp Val Val Pro Gly Arg Leu Thr Ser Met 
785                 790                 795                 800 


Leu Arg Gly Lys Trp Gly Leu Asn Gly Leu Leu Pro Ser Asp Asn Tyr 
                805                 810                 815     


Ala Gly Val Gln Asp Lys Ala Glu Glu Phe Leu Ala Ser Thr Asp Asp 
            820                 825                 830         


Met Glu Phe Ser Gly Val Lys Asn Arg Ala Asp His Arg His His Ala 
        835                 840                 845             


Ile Asp Gly Leu Val Thr Ala Leu Thr Asp Arg Ser Leu Leu Trp Lys 
    850                 855                 860                 


Met Ala Asn Ala Tyr Asp Glu Glu His Glu Lys Phe Val Ile Glu Pro 
865                 870                 875                 880 


Pro Trp Pro Thr Met Arg Asp Asp Leu Lys Ala Ala Leu Glu Lys Met 
                885                 890                 895     


Val Val Ser His Lys Pro Asp His Gly Ile Glu Gly Lys Leu His Glu 
            900                 905                 910         


Asp Ser Ala Tyr Gly Phe Val Lys Pro Leu Asp Ala Thr Gly Leu Lys 
        915                 920                 925             


Glu Glu Glu Ala Gly Asn Leu Val Tyr Arg Lys Ala Ile Glu Ser Leu 
    930                 935                 940                 


Asn Glu Asn Glu Val Asp Arg Ile Arg Asp Ile Gln Leu Arg Thr Ile 
945                 950                 955                 960 


Val Arg Asp His Val Asn Val Glu Lys Thr Lys Gly Val Ala Leu Ala 
                965                 970                 975     


Asp Ala Leu Arg Gln Leu Gln Ala Pro Ser Asp Asp Tyr Pro Gln Phe 
            980                 985                 990         


Lys His Gly Leu Arg His Val Arg  Ile Leu Lys Lys Glu  Lys Gly Asp 
        995                 1000                 1005             


Tyr Leu  Val Pro Ile Ala Asn  Arg Ala Ser Gly Val  Ala Tyr Lys 
    1010                 1015                 1020             


Ala Tyr  Ser Ala Gly Glu Asn  Phe Cys Val Glu Val  Phe Glu Thr 
    1025                 1030                 1035             


Ala Gly  Gly Lys Trp Asp Gly  Glu Ala Val Arg Arg  Phe Asp Ala 
    1040                 1045                 1050             


Asn Lys  Lys Asn Ala Gly Pro  Lys Ile Ala His Ala  Pro Gln Trp 
    1055                 1060                 1065             


Arg Asp  Ala Asn Glu Gly Ala  Lys Leu Val Met Arg  Ile His Lys 
    1070                 1075                 1080             


Gly Asp  Leu Ile Arg Leu Asp  His Glu Gly Arg Ala  Arg Ile Met 
    1085                 1090                 1095             


Val Val  His Arg Leu Asp Ala  Ala Ala Gly Arg Phe  Lys Leu Ala 
    1100                 1105                 1110             


Asp His  Asn Glu Thr Gly Asn  Leu Asp Lys Arg His  Ala Thr Asn 
    1115                 1120                 1125             


Asn Asp  Ile Asp Pro Phe Arg  Trp Leu Met Ala Ser  Tyr Asn Thr 
    1130                 1135                 1140             


Leu Lys  Lys Leu Ala Ala Val  Pro Val Arg Val Asp  Glu Leu Gly 
    1145                 1150                 1155             


Arg Val  Trp Arg Val Met Pro  Asn 
    1160                 1165     


<210>  751
<211>  1168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  751

Met Ala Arg Pro Ala Phe Arg Ala Pro Arg Arg Glu His Val Asn Gly 
1               5                   10                  15      


Trp Thr Pro Asp Pro His Arg Ile Ser Lys Pro Phe Phe Ile Leu Val 
            20                  25                  30          


Ser Trp His Leu Leu Ser Arg Val Val Ile Asp Ser Ser Ser Gly Cys 
        35                  40                  45              


Pro Phe Gly Thr Ser Arg Asp His Thr Asp Lys Phe Ala Glu Trp Glu 
    50                  55                  60                  


Cys Ala Val Gln Pro Tyr Arg Leu Ser Phe Asp Leu Gly Thr Asn Ser 
65                  70                  75                  80  


Ile Gly Trp Gly Leu Leu Asn Leu Asp Arg Gln Gly Lys Pro Arg Glu 
                85                  90                  95      


Ile Arg Ala Leu Gly Ser Arg Ile Phe Ser Asp Gly Arg Asp Pro Gln 
            100                 105                 110         


Asp Lys Ala Ser Leu Ala Val Ala Arg Arg Leu Ala Arg Gln Met Arg 
        115                 120                 125             


Arg Arg Arg Asp Arg Tyr Leu Thr Arg Arg Thr Arg Leu Met Gly Ala 
    130                 135                 140                 


Leu Val Arg Phe Gly Leu Met Pro Ala Asp Pro Ala Ala Arg Lys Arg 
145                 150                 155                 160 


Leu Glu Val Ala Val Asp Pro Tyr Leu Ala Arg Glu Arg Ala Thr Arg 
                165                 170                 175     


Glu Arg Leu Glu Pro Phe Glu Ile Gly Arg Ala Leu Phe His Leu Asn 
            180                 185                 190         


Gln Arg Arg Gly Tyr Lys Pro Val Arg Thr Ala Thr Lys Pro Asp Glu 
        195                 200                 205             


Glu Ala Gly Lys Val Lys Glu Ala Val Glu Arg Leu Glu Ala Ala Ile 
    210                 215                 220                 


Ala Ala Ala Gly Ala Pro Thr Leu Gly Ala Trp Phe Ala Trp Arg Lys 
225                 230                 235                 240 


Thr Arg Gly Glu Thr Leu Arg Ala Arg Leu Ala Gly Lys Gly Lys Glu 
                245                 250                 255     


Ala Ala Tyr Pro Phe Tyr Pro Ala Arg Arg Met Leu Glu Ala Glu Phe 
            260                 265                 270         


Asp Thr Leu Trp Ala Glu Gln Ala Arg His His Pro Asp Leu Leu Thr 
        275                 280                 285             


Ala Glu Ala Arg Glu Ile Leu Arg His Arg Ile Phe His Gln Arg Pro 
    290                 295                 300                 


Leu Lys Pro Pro Pro Val Gly Arg Cys Thr Leu Tyr Pro Asp Asp Gly 
305                 310                 315                 320 


Arg Ala Pro Arg Ala Leu Pro Ser Ala Gln Arg Leu Arg Leu Phe Gln 
                325                 330                 335     


Glu Leu Ala Ser Leu Arg Val Ile His Leu Asp Leu Ser Glu Arg Pro 
            340                 345                 350         


Leu Thr Pro Ala Glu Arg Asp Arg Ile Val Ala Phe Val Gln Gly Arg 
        355                 360                 365             


Pro Pro Lys Ala Gly Arg Lys Pro Gly Lys Val Gln Lys Ser Val Pro 
    370                 375                 380                 


Phe Glu Lys Leu Arg Gly Leu Leu Glu Leu Pro Pro Gly Thr Gly Phe 
385                 390                 395                 400 


Ser Leu Glu Ser Asp Lys Arg Pro Glu Leu Leu Gly Asp Glu Thr Gly 
                405                 410                 415     


Ala Arg Ile Ala Pro Ala Phe Gly Pro Gly Trp Thr Ala Leu Pro Leu 
            420                 425                 430         


Glu Glu Gln Asp Ala Leu Val Glu Leu Leu Leu Thr Glu Ala Glu Pro 
        435                 440                 445             


Glu Arg Ala Ile Ala Ala Leu Thr Ala Arg Trp Ala Leu Asp Glu Ala 
    450                 455                 460                 


Thr Ala Ala Lys Leu Ala Gly Ala Thr Leu Pro Asp Phe His Gly Arg 
465                 470                 475                 480 


Tyr Gly Arg Arg Ala Val Ala Glu Leu Leu Pro Val Leu Glu Arg Glu 
                485                 490                 495     


Thr Arg Gly Asp Pro Asp Gly Arg Val Arg Pro Ile Arg Leu Asp Glu 
            500                 505                 510         


Ala Val Lys Leu Leu Arg Gly Gly Lys Asp His Ser Asp Phe Ser Arg 
        515                 520                 525             


Glu Gly Ala Leu Leu Asp Ala Leu Pro Tyr Tyr Gly Ala Val Leu Glu 
    530                 535                 540                 


Arg His Val Ala Phe Gly Thr Gly Asn Pro Ala Asp Pro Glu Glu Lys 
545                 550                 555                 560 


Arg Val Gly Arg Val Ala Asn Pro Thr Val His Ile Ala Leu Asn Gln 
                565                 570                 575     


Leu Arg His Leu Val Asn Ala Ile Leu Ala Arg His Gly Arg Pro Glu 
            580                 585                 590         


Glu Ile Val Ile Glu Leu Ala Arg Asp Leu Lys Arg Ser Ala Glu Asp 
        595                 600                 605             


Arg Arg Arg Glu Asp Lys Arg Gln Ala Asp Asn Gln Lys Arg Asn Glu 
    610                 615                 620                 


Glu Arg Lys Arg Leu Ile Leu Ser Leu Gly Glu Arg Pro Thr Pro Arg 
625                 630                 635                 640 


Asn Leu Leu Lys Leu Arg Leu Trp Glu Glu Gln Gly Pro Val Glu Asn 
                645                 650                 655     


Arg Arg Cys Pro Tyr Ser Gly Glu Thr Ile Ser Met Arg Met Leu Leu 
            660                 665                 670         


Ser Glu Gln Val Asp Ile Asp His Ile Leu Pro Phe Ser Val Ser Leu 
        675                 680                 685             


Asp Asp Ser Ala Ala Asn Lys Val Val Cys Leu Arg Glu Ala Asn Arg 
    690                 695                 700                 


Ile Lys Arg Asn Arg Ser Pro Trp Glu Ala Phe Gly His Asp Ser Glu 
705                 710                 715                 720 


Arg Trp Ala Gly Ile Leu Ala Arg Ala Glu Ala Leu Pro Lys Asn Lys 
                725                 730                 735     


Arg Trp Arg Phe Ala Pro Asp Ala Leu Glu Lys Leu Glu Gly Glu Gly 
            740                 745                 750         


Gly Leu Arg Ala Arg His Leu Asn Asp Thr Arg His Leu Ser Arg Leu 
        755                 760                 765             


Ala Val Glu Tyr Leu Arg Cys Val Cys Pro Lys Val Arg Val Ser Pro 
    770                 775                 780                 


Gly Arg Leu Thr Ala Leu Leu Arg Arg Arg Trp Gly Ile Asp Ala Ile 
785                 790                 795                 800 


Leu Ala Glu Ala Asp Gly Pro Pro Pro Glu Val Pro Ala Glu Thr Leu 
                805                 810                 815     


Asp Pro Ser Pro Ala Glu Lys Asn Arg Ala Asp His Arg His His Ala 
            820                 825                 830         


Leu Asp Ala Val Val Ile Gly Cys Ile Asp Arg Ser Met Val Gln Arg 
        835                 840                 845             


Val Gln Leu Ala Ala Ala Ser Ala Glu Arg Glu Ala Ala Ala Arg Glu 
    850                 855                 860                 


Asp Asn Ile Arg Arg Val Leu Glu Gly Phe Lys Glu Glu Pro Trp Asp 
865                 870                 875                 880 


Gly Phe Arg Ala Glu Leu Glu Arg Arg Ala Arg Thr Ile Val Val Ser 
                885                 890                 895     


His Arg Pro Glu His Gly Ile Gly Gly Ala Leu His Lys Glu Thr Ala 
            900                 905                 910         


Tyr Gly Pro Val Asp Pro Pro Glu Glu Gly Phe Asn Leu Val Val Arg 
        915                 920                 925             


Lys Pro Ile Asp Gly Leu Ser Lys Asp Glu Ile Asn Ser Val Arg Asp 
    930                 935                 940                 


Pro Arg Leu Arg Arg Ala Leu Ile Asp Arg Leu Ala Ile Arg Arg Arg 
945                 950                 955                 960 


Asp Ala Asn Asp Pro Ala Thr Ala Leu Ala Lys Ala Ala Glu Asp Leu 
                965                 970                 975     


Ala Ala Gln Pro Ala Ser Arg Gly Ile Arg Arg Val Arg Val Leu Lys 
            980                 985                 990         


Lys Glu Ser Asn Pro Ile Arg Val  Glu His Gly Gly Asn  Pro Ser Gly 
        995                 1000                 1005             


Pro Arg  Ser Gly Gly Pro Phe  His Lys Leu Leu Leu  Ala Gly Glu 
    1010                 1015                 1020             


Val His  His Val Asp Val Ala  Leu Arg Ala Asp Gly  Arg Arg Trp 
    1025                 1030                 1035             


Val Gly  His Trp Val Thr Leu  Phe Glu Ala His Gly  Gly Arg Gly 
    1040                 1045                 1050             


Ala Asp  Gly Ala Ala Ala Pro  Pro Arg Leu Gly Asp  Gly Glu Arg 
    1055                 1060                 1065             


Phe Leu  Met Arg Leu His Lys  Gly Asp Cys Leu Lys  Leu Glu His 
    1070                 1075                 1080             


Lys Gly  Arg Val Arg Val Met  Gln Val Val Lys Leu  Glu Pro Ser 
    1085                 1090                 1095             


Ser Asn  Ser Val Val Val Val  Glu Pro His Gln Val  Lys Thr Asp 
    1100                 1105                 1110             


Arg Ser  Lys His Val Lys Ile  Ser Cys Asp Gln Leu  Arg Ala Arg 
    1115                 1120                 1125             


Gly Ala  Arg Arg Val Thr Val  Asp Pro Leu Gly Arg  Val Arg Val 
    1130                 1135                 1140             


His Ala  Pro Gly Ala Arg Val  Gly Ile Gly Gly Asp  Ala Gly Arg 
    1145                 1150                 1155             


Thr Ala  Met Glu Pro Ala Glu  Asp Ile Ser 
    1160                 1165             


<210>  752
<211>  1170
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  752

Met Ser Lys Tyr Asn Val Gly Leu Asp Ile Gly Ile Ala Ser Val Gly 
1               5                   10                  15      


Trp Ser Ile Val Asp Ala Asp Lys Lys Lys Ile Ile Asp Leu Gly Ser 
            20                  25                  30          


Arg Ile Phe Ser Ser Gly Asn Ala Ala Ala Asn Gln Glu Arg Arg Gly 
        35                  40                  45              


Phe Arg Gly Thr Arg Arg Leu Ile Arg Arg Arg Lys Asn Arg Leu Thr 
    50                  55                  60                  


Asp Val Ile Asn Phe Phe Gly Glu Lys Lys Phe Ile Asp Thr Lys Val 
65                  70                  75                  80  


Val Asp Lys Asn Gly His Val His Tyr Lys Phe Ser Val Asn Asn Thr 
                85                  90                  95      


Glu Ser Pro Tyr Glu Leu Arg Val Lys Gly Leu Ser Glu Ile Leu Thr 
            100                 105                 110         


Lys Gln Glu Leu Val Ile Ala Leu Tyr His Ile Val Lys His Arg Gly 
        115                 120                 125             


Ile Ser Tyr Asp Leu Gly Asp Leu Glu Asp Asp Gly Thr Ser Gly Val 
    130                 135                 140                 


Thr Asp Tyr Lys Thr Ser Ile Asn Ile Asn Arg Gln Leu Leu Asn Glu 
145                 150                 155                 160 


Lys Thr Val Gly Gln Ile Gln Leu Glu Arg Leu Asn Glu Phe Gly Lys 
                165                 170                 175     


Val Arg Gly Gln Val Glu Glu Asn Glu Asn Val Thr Leu Leu Asn Val 
            180                 185                 190         


Phe Pro Ser Ala Ala Tyr Val Lys Glu Ala Thr Gln Ile Leu Thr Lys 
        195                 200                 205             


Gln Arg Glu Phe Tyr Pro Glu Ile Thr Asp Asp Phe Ile Ala Lys Phe 
    210                 215                 220                 


Met Leu Phe Ile Thr Arg Lys Arg Asp Tyr Phe Val Gly Pro Gly Asn 
225                 230                 235                 240 


Glu Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Lys Lys Asp Gly Arg Thr 
                245                 250                 255     


Leu Asp Asn Leu Phe Asp Glu Leu Ile Gly Ile Asp Lys Ile Asn Gly 
            260                 265                 270         


Glu Lys Arg Ala Ser Ala Ser Ser Leu Thr Ala Gln Ile Tyr Asn Met 
        275                 280                 285             


Leu Asn Asp Leu Asn Asn Leu Thr Val Pro Asn Thr Glu Asp Gly Arg 
    290                 295                 300                 


Leu Thr Ile Gln Thr Lys Lys Glu Ile Leu Glu Ala Ala Lys Thr Thr 
305                 310                 315                 320 


Asn Gly Leu Phe Gly Ile Ala Gln Ile Cys Lys Ile Ile Gly Cys Lys 
                325                 330                 335     


Lys Asp Asp Ile Lys Gly Phe Arg Ile Asp Lys Ser Glu Lys Lys Ile 
            340                 345                 350         


Met His Thr Leu Ser Gly Tyr Arg Lys Phe Arg Lys Glu Leu Ala Asp 
        355                 360                 365             


Val Asn Ile Glu Ala Asn Asp Leu Asn Ile Asn Leu Val Asn Lys Ile 
    370                 375                 380                 


Ala Asp Ile Leu Thr Leu Asn Thr Glu Lys Ala Glu Ile Arg Lys Gln 
385                 390                 395                 400 


Leu Asp Lys Ser Asn Met Asp Ile Ser Asn Glu Met Lys Glu Ile Val 
                405                 410                 415     


Ile Asn Lys Asn Lys Glu Phe Leu Val Asp Gly Arg Ala Thr Trp His 
            420                 425                 430         


Ser Phe Ser Tyr Lys Thr Leu Asn Leu Leu Ile Pro Glu Leu Leu Asn 
        435                 440                 445             


Thr Pro Glu Glu Gln Met Thr Ile Leu Thr Arg Leu Gly Leu Val Lys 
    450                 455                 460                 


Pro Asn Asn Lys Lys Tyr Glu Gly Leu Lys Asn Ile Pro Ala Arg Arg 
465                 470                 475                 480 


Ile Thr Glu Glu Ile Tyr Asn Pro Val Val Ala Arg Ala Thr Arg Glu 
                485                 490                 495     


Ala Val Asn Val Phe Asn Ala Ile Thr Lys Lys Val Gly Arg Asp Asn 
            500                 505                 510         


Ile Lys Asp Val Ile Ile Glu Leu Pro Arg Glu Asp Asn Glu Val Asp 
        515                 520                 525             


Ile Lys Lys Gly Ile Val Lys Arg Gln Lys Gln Asn Glu Val Glu Lys 
    530                 535                 540                 


Glu Thr Ala Asp Ala Leu Val Lys Gln His Met Thr Ile Ser Asp Ser 
545                 550                 555                 560 


Ala Leu Ile Ala Gln Tyr Arg Lys Ile Arg Gly Leu Ser Gln Lys Val 
                565                 570                 575     


Arg Tyr Trp Tyr Gln Gln Asp Thr Ile Cys Pro Tyr Cys Gly Lys Lys 
            580                 585                 590         


Ile Glu Ala Val Gln Leu Ile Asn Asn Asn Asp Ser Phe Glu Val Asp 
        595                 600                 605             


His Ile Val Pro Ile Ser Val Ser Phe Asp Asp Ser Gln Asn Asn Lys 
    610                 615                 620                 


Val Leu Val His Ser Gln Cys Asn Gln Ala Lys Glu Lys Gln Thr Pro 
625                 630                 635                 640 


Leu Gly Trp Leu Asn Asn Gly Gly Gly Phe Gly Gln Ser Lys Ala Asp 
                645                 650                 655     


Tyr Ile Ala Arg Val Lys Ser Asn Lys Asn Tyr Ala Lys Asn Lys Ile 
            660                 665                 670         


Asp Asn Leu Leu Asn Glu Thr Asp Leu Asn Asp Ile Ala Thr Gln Arg 
        675                 680                 685             


Gly Phe Ile Gln Arg Asn Leu Asn Asp Thr Arg Tyr Ala Ser Arg Ile 
    690                 695                 700                 


Val Leu Asn Glu Phe Tyr Ser Phe Phe Arg Ser Asn Asn Leu Ser Thr 
705                 710                 715                 720 


Lys Val Lys Val Val Arg Gly Lys Trp Thr Ser Gln Met Arg Lys Lys 
                725                 730                 735     


Trp Asn Gly Val Gly Gly Leu Ser Lys Thr Arg Asp Thr Tyr His His 
            740                 745                 750         


His Ala Ile Asp Ala Cys Ile Ile Ala Cys Pro Phe Leu Leu Lys Ala 
        755                 760                 765             


Phe Asp Lys Ala Ile Lys Leu Ile Asp Val Asp Gly Glu Thr Gly Glu 
    770                 775                 780                 


Ile Leu Gln Asp Lys Lys Ala Ile Lys Leu Ala Gln Glu Ala Glu Ile 
785                 790                 795                 800 


Val Glu Lys Ile Ser Val Ile Lys Asn Lys Lys Phe Glu Asn Met Val 
                805                 810                 815     


Asn Glu Ile Tyr Asn Phe Pro Leu Phe Lys Gln Val Glu Leu Ala Asn 
            820                 825                 830         


Asp Ile Thr Asn Leu Glu Asn Pro Val Lys Phe Ser His His Val Asp 
        835                 840                 845             


Lys Lys Ala Asn Arg Ala Val Ala Asn Gln Thr Ile Tyr Gly Thr Arg 
    850                 855                 860                 


Ile Lys Glu Lys Glu Val Thr Lys Arg Gly Lys Lys Glu Lys Ile Gln 
865                 870                 875                 880 


Glu Arg Tyr Thr Leu Gly Thr Ile Lys Asn Ile Tyr Asp Ile Asp Gly 
                885                 890                 895     


Phe Met Asn Phe Lys Lys Leu Tyr Asp Lys Ile Ser Lys Asn Asp Asp 
            900                 905                 910         


Val Lys Phe Leu Met Gln Lys Asn Asp Pro Lys Thr Trp Glu Lys Leu 
        915                 920                 925             


Ile Asp Ile Phe Asn Ser Tyr Pro Asp Ser Glu Glu Val Thr Gln Val 
    930                 935                 940                 


Asp Gly Lys Val Lys Arg Val Ala Val Ser Pro Phe Lys Leu Tyr Tyr 
945                 950                 955                 960 


Arg Glu Asn Gly Phe Ile Thr Lys Tyr Ser Lys His Asn Asp Gly Pro 
                965                 970                 975     


Lys Val Val Ser Ile Lys Tyr Tyr Asp Ser Lys Val Gly Ser His Ile 
            980                 985                 990         


Asp Ile Thr Pro Lys Asp Ala Arg  Asn Lys Val Ile Leu  Gln Ser Leu 
        995                 1000                 1005             


Lys Pro  Trp Arg Thr Asp Val  Tyr Phe Asn Ser Ile  Thr Gln Gln 
    1010                 1015                 1020             


Tyr Glu  Leu Leu Gly Leu Lys  Tyr Ser Asp Leu Lys  Phe Thr Asn 
    1025                 1030                 1035             


Gly Val  Tyr Gly Ile Thr Arg  Asp Ala Tyr Glu Lys  Leu Lys His 
    1040                 1045                 1050             


Gly Val  Ser Glu Asp Gly Val  Thr Ala Trp Lys Pro  Ile Ser Lys 
    1055                 1060                 1065             


Asn Ser  Glu Phe Arg Phe Ser  Leu Tyr Arg Asn Asp  Arg Val Arg 
    1070                 1075                 1080             


Val Ile  Asp Glu Lys Gly Asp  Ser Ile Glu Leu Leu  Phe Ala Ser 
    1085                 1090                 1095             


Arg Thr  Thr Ser Asn Glu Gly  Tyr Val Glu Leu Lys  Pro Ile Tyr 
    1100                 1105                 1110             


Lys Ala  Lys Phe Asp Ser Lys  Glu Glu Val Gly Phe  Tyr Gly Leu 
    1115                 1120                 1125             


Val Ser  Asn Gly Arg Leu Ile  Lys Lys Met Ser Lys  Gly Gly Tyr 
    1130                 1135                 1140             


Lys Leu  Tyr Lys Val Asn Thr  Asp Ile Leu Gly Lys  Pro Tyr Tyr 
    1145                 1150                 1155             


Val Thr  Arg Glu Ser Asp Glu  Pro Lys Leu Ser Leu  
    1160                 1165                 1170 


<210>  753
<211>  1171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  753

Met Ala Lys Tyr Ser Val Gly Leu Asp Ile Gly Ile Ser Ser Val Gly 
1               5                   10                  15      


Trp Ala Ile Ile Asp Ile Glu Thr Lys Gln Ile Val Asn Leu Gly Ser 
            20                  25                  30          


Arg Ile Phe Pro Ser Gly Asn Ala Ala Gly Asn Gln Glu Arg Arg Gly 
        35                  40                  45              


Phe Arg Gly Ile Arg Arg Leu Ile Arg Arg Arg Lys Asn Arg Leu Thr 
    50                  55                  60                  


Asp Leu Lys Lys Leu Leu Gly Glu Thr Phe Phe Ser Asp Leu Lys Lys 
65                  70                  75                  80  


Ile Asp Ser Asn Gly Tyr Glu Tyr Tyr Asp Ile Asp Phe Gln Ile Asn 
                85                  90                  95      


Ala Asn Pro Tyr Glu Leu Arg Val Lys Gly Ile Thr Glu Lys Leu Ser 
            100                 105                 110         


Lys Lys Glu Leu Ala Val Val Leu His His Ile Ile Lys His Arg Gly 
        115                 120                 125             


Ile Ser Tyr Asp Leu Gly Asp Leu Glu Asp Asp Gly Thr Ser Gly Val 
    130                 135                 140                 


Ser Asp Tyr Lys Asn Ser Ile Asn Ile Asn Arg Gln Leu Leu Arg Glu 
145                 150                 155                 160 


Lys Thr Val Gly Gln Ile Gln Leu Glu Arg Leu Ile Glu Phe Asn Gln 
                165                 170                 175     


Val His Gly Gln Val Lys Lys Ala Glu Asp Val Ser Leu Leu Asn Val 
            180                 185                 190         


Phe Pro Ser Ser Ala Tyr Arg Asp Glu Ala Asn Lys Ile Met Ile Lys 
        195                 200                 205             


Gln Gln Glu Phe Tyr Ser Glu Ile Thr Asn Ser Phe Met Glu Gln Val 
    210                 215                 220                 


Asp Gln Leu Val Ser Arg Lys Arg Asp Tyr Phe Ile Gly Pro Gly Asn 
225                 230                 235                 240 


Glu Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Lys Ser Asp Gly Arg Thr 
                245                 250                 255     


Leu Asp Asn Leu Phe Ala Glu Leu Ile Gly Thr Asp Lys Ile Asn Gly 
            260                 265                 270         


Glu Lys Arg Ala Ser Ala Ser Ser Leu Thr Ala Gln Val Tyr Asn Leu 
        275                 280                 285             


Leu Asn Asp Leu Asn Asn Leu His Val Ser Asn Thr Glu Asp Gly Lys 
    290                 295                 300                 


Leu Thr Thr Ser Thr Lys Lys Glu Ile Ile Lys Ala Ala Lys Thr Thr 
305                 310                 315                 320 


Asp Gly Val Phe Gly Ile Ser Gln Ile Cys Lys Ile Ile Gly Cys Lys 
                325                 330                 335     


Lys Glu Glu Ile Thr Gly Phe Arg Gln Asp Lys Ser Glu Lys Lys Val 
            340                 345                 350         


Ile His Thr Leu Ala Gly Tyr Arg Lys Ile Arg Lys Ala Leu Ala Thr 
        355                 360                 365             


Ile Asn Ile Glu Ala Asn Glu Leu Asn Pro Thr Val Phe Asp Gln Ile 
    370                 375                 380                 


Ala Asp Ile Leu Thr Leu Asn Thr Glu Lys Ala Glu Ile Arg Lys Gln 
385                 390                 395                 400 


Leu Asp Lys Val Gln Leu Asp Ile Thr Asp Glu Met Lys Glu Leu Val 
                405                 410                 415     


Ile Asp Lys Asn Lys Asp Phe Leu Val Asp Gly Arg Ala Thr Trp His 
            420                 425                 430         


Ser Phe Ser Tyr Gln Thr Leu Asn Met Leu Ile Pro Glu Leu Leu Asn 
        435                 440                 445             


Thr Ser Glu Glu Gln Met Thr Ile Leu Thr Arg Met Gly Leu Met Lys 
    450                 455                 460                 


Ser Asp Asn Glu Lys Tyr Arg Gly Leu Lys Lys Ile Pro Ala Glu Lys 
465                 470                 475                 480 


Ile Ala Ser Glu Ile Phe Asn Pro Val Val Ala Lys Ser Thr Arg Glu 
                485                 490                 495     


Ala Ile Lys Val Phe Asn Ala Ile Ser Lys Glu Ile Gly Lys Asn Asn 
            500                 505                 510         


Ile Glu His Val Val Ile Glu Leu Pro Arg Asp Asp Asn Glu Glu Asp 
        515                 520                 525             


Glu Lys Arg Lys Ile Gln Lys Leu Gln Lys Ala Asn Glu Thr Glu Lys 
    530                 535                 540                 


Glu Ala Ala Asp Asn Leu Val Lys His Lys Leu Gly Ile Ser Asp Ser 
545                 550                 555                 560 


Ala Leu Ile Ala His Tyr Arg Lys Phe Lys Gly Leu Ser Gln Lys Val 
                565                 570                 575     


Arg Tyr Trp Tyr Gln Gln Glu Thr Tyr Cys Pro Tyr Cys Gly Lys Ile 
            580                 585                 590         


Ile Glu Ala Thr Gln Leu Ile Asn Asn Asn Asp Asn Phe Glu Val Asp 
        595                 600                 605             


His Ile Ile Pro Ile Ser Val Ser Tyr Asp Asp Ser Gln Asn Asn Lys 
    610                 615                 620                 


Val Leu Val His Ser Gln Cys Asn Gln Ala Lys Glu Lys Gln Thr Pro 
625                 630                 635                 640 


Leu Gly Trp Ile Asn Asn Gly Gly Gly Phe Gly Gln Ser Lys Asn Glu 
                645                 650                 655     


Tyr Thr Asn Arg Val Lys Asn Asn Lys Lys Tyr Ser Lys Asn Lys Val 
            660                 665                 670         


Asp Asn Leu Leu Asn Glu Thr Asp Leu Thr Asp Ile Ile Thr Gln Arg 
        675                 680                 685             


Ser Phe Ile Gln Arg Asn Leu Asn Asp Thr Arg Tyr Ala Ala Arg Thr 
    690                 695                 700                 


Val Leu Asp Glu Phe Tyr Ser Phe Phe Lys Ser Asn Asp Leu Pro Thr 
705                 710                 715                 720 


Lys Val Lys Val Val Arg Gly Lys Trp Thr Ser Gln Met Arg Arg Lys 
                725                 730                 735     


Trp Asn Asn Val Gly Gly Leu Arg Lys Thr Arg Asp Thr His His His 
            740                 745                 750         


His Ala Ile Asp Ala Ser Ile Ile Ala Ser Phe Pro Leu Leu Arg Ala 
        755                 760                 765             


Phe Asp Arg Ala Ile Lys Leu Ile Asp Val Asp Lys Glu Thr Gly Glu 
    770                 775                 780                 


Ile Val Gln Tyr Gly Lys Ala Ser Leu Asn Gly Asp Val Ile Glu Glu 
785                 790                 795                 800 


Lys Ser Val Ile Gln Ala Pro Ile Ile Lys Asn Glu Lys Phe Glu Lys 
                805                 810                 815     


Leu Val Asp Glu Ile Tyr Asn Phe Pro Leu Phe Lys Gln Val Glu Leu 
            820                 825                 830         


Ala Asn Asn Ile Glu Asn Pro Glu Asn Pro Val Lys Phe Ser His His 
        835                 840                 845             


Val Asp Lys Lys Ala Asn Arg Thr Val Ala Asn Gln Thr Ile Tyr Ser 
    850                 855                 860                 


Thr Arg Ile Lys Lys Thr Glu Lys Ile Val Arg Lys Lys Val Thr Ala 
865                 870                 875                 880 


Lys Glu Glu Glu Tyr Val Ile Gly Thr Ile Lys Asp Ile Tyr Asp Val 
                885                 890                 895     


Thr Asn Phe Glu Lys Phe Lys Lys Leu Tyr Asp Glu Ser Ser Lys Lys 
            900                 905                 910         


Asp Asp Ile Lys Phe Leu Met Gln Lys Asn Asp Pro Lys Thr Trp Glu 
        915                 920                 925             


Lys Leu Val Glu Ile Met Asn Thr Tyr Pro Asp Tyr Glu Glu Ile Ser 
    930                 935                 940                 


Gln Leu Asp Gly Lys Val Lys Arg Val Lys Val Ser Pro Phe Lys Leu 
945                 950                 955                 960 


Tyr Phe Arg Glu Asn Gly Phe Ile Thr Lys Tyr Ala Lys His Asn Asn 
                965                 970                 975     


Gly Pro Lys Val Val Ser Ile Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
            980                 985                 990         


His Ile Asp Ile Thr Pro Val Asn  Ala Asn Asn Lys Val  Ile Leu Gln 
        995                 1000                 1005             


Ser Leu  Lys Pro Trp Arg Thr  Asp Val Tyr Tyr Asn  Asn Glu Thr 
    1010                 1015                 1020             


Gln Gln  Tyr Glu Leu Leu Gly  Leu Lys Tyr Ser Asp  Leu Lys Phe 
    1025                 1030                 1035             


Thr Lys  Gly Val Tyr Gly Ile  Thr Arg Glu Ala Tyr  Glu Lys Leu 
    1040                 1045                 1050             


Lys Tyr  Gly Tyr Thr Asp Gly  Val Pro Ala Trp Lys  Pro Ile Ser 
    1055                 1060                 1065             


Lys Glu  Ser Glu Phe Lys Phe  Ser Leu Tyr Arg Asn  Asp Arg Val 
    1070                 1075                 1080             


Arg Ile  Ile Asn Glu Ala Gly  Glu Lys Ile Asp Leu  Leu Phe Gly 
    1085                 1090                 1095             


Ser Arg  Asn Glu Thr Ser Glu  Gly Tyr Val Glu Met  Lys Pro Ile 
    1100                 1105                 1110             


Phe Lys  Thr Asn Phe Asp Asp  Ala Glu Glu Ala Ser  Phe Tyr Gly 
    1115                 1120                 1125             


Lys Thr  Ser Asn Lys Arg Leu  Ile Lys Lys Met Ala  Arg Lys Gly 
    1130                 1135                 1140             


Tyr Gln  Ile Tyr Lys Val Asn  Thr Asp Ile Leu Gly  Asn Leu Tyr 
    1145                 1150                 1155             


Tyr Thr  Ala Lys Glu Ser Asn  Glu Pro Lys Leu Asp  Leu 
    1160                 1165                 1170     


<210>  754
<211>  1172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  754

Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Arg Ile Gln Gly Thr Thr Asp Arg Thr Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Asn Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Lys Tyr Arg Ile Lys 
65                  70                  75                  80  


Glu Leu Gln Lys Ile Phe Ser Ser Glu Met Asn Glu Leu Asp Ile Ala 
                85                  90                  95      


Phe Phe Pro Arg Leu Ser Glu Ser Phe Leu Val Ser Asp Asp Lys Glu 
            100                 105                 110         


Phe Glu Asn His Pro Ile Phe Gly Asn Leu Lys Asp Glu Ile Thr Tyr 
        115                 120                 125             


His Asn Asp Tyr Pro Thr Ile Tyr His Leu Arg Gln Thr Leu Ala Asp 
    130                 135                 140                 


Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asn Leu Asp Ser 
                165                 170                 175     


Glu Asn Thr Asp Val His Val Leu Phe Leu Asn Leu Val Asn Ile Tyr 
            180                 185                 190         


Asn Asn Leu Phe Glu Glu Asp Ile Val Glu Thr Ala Ser Ile Asp Ala 
        195                 200                 205             


Glu Lys Ile Leu Thr Ser Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Glu Ile Pro Asn Gln Lys Arg Asn Met Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Val Ser Leu Ala Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     


Glu Leu Leu Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Ser Tyr Glu 
            260                 265                 270         


Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Ile Ala Ala Lys Lys Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Ile Thr Val Lys Gly Ala Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Val Gln Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     


Asn Leu Val Lys Lys Gln Ile Pro Glu Lys Tyr Lys Glu Ile Phe Asp 
            340                 345                 350         


Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Leu Lys Leu Asp 
    370                 375                 380                 


Gly Thr Glu Lys Leu Ile Ser Lys Leu Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Asn Glu Leu Lys Ala Ile Ile Arg Arg Gln Glu Lys Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Glu Asn Gln Lys Lys Ile Glu Lys Leu Phe Thr Phe Lys Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Gln Ser Ser Phe Ala Trp 
    450                 455                 460                 


Leu Lys Arg Gln Ser Asn Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Gln Glu Ala Ser Ala Arg Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Pro Leu Tyr Glu Met Phe Met Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Gln Thr Glu Gly Met Lys Arg Pro Val Phe Leu Ser Ser Glu Asp 
    530                 535                 540                 


Lys Glu Glu Ile Val Asn Leu Leu Phe Lys Lys Glu Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Glu Tyr Phe Ser Lys Met Lys Cys Phe His 
                565                 570                 575     


Thr Val Thr Ile Leu Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Phe Lys Asp Lys Ala Phe Leu Asp 
        595                 600                 605             


Asp Glu Ala Asn Gln Asp Ile Leu Glu Glu Ile Val Trp Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Gln Ala Met Ile Glu Arg Arg Leu Val Lys Tyr Ala 
625                 630                 635                 640 


Asp Val Phe Glu Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Gln Lys Leu Ile Asn Gly Ile Lys Asp 
            660                 665                 670         


Lys Gln Thr Gly Lys Thr Ile Leu Gly Phe Leu Lys Asp Asp Gly Val 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe 
    690                 695                 700                 


Ala Lys Ile Ile Lys Asn Glu Gln Glu Lys Thr Ile Lys Asn Glu Ser 
705                 710                 715                 720 


Leu Glu Glu Thr Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Ile Met 
            740                 745                 750         


Gly Gln Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Ser Thr Met Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Arg Lys Leu 
    770                 775                 780                 


Glu Glu Val His Lys Asn Thr Gly Ser Lys Ile Leu Lys Glu Tyr Asn 
785                 790                 795                 800 


Val Ser Asn Thr Gln Leu Gln Ser Asp Arg Leu Tyr Leu Tyr Leu Leu 
                805                 810                 815     


Gln Asp Gly Lys Asp Met Tyr Thr Gly Lys Glu Leu Asp Tyr Asp Asn 
            820                 825                 830         


Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             


Asp Asn Ser Ile Asp Asn Thr Val Leu Thr Thr Gln Ala Ser Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Asn Ile Glu Thr Val Asn Lys Met Lys 
865                 870                 875                 880 


Ser Phe Trp Tyr Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys 
                885                 890                 895     


Phe Asp His Leu Thr Lys Ala Glu Arg Gly Ala Leu Ser Asp Phe Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Asn Leu Thr 
    930                 935                 940                 


Glu Asp Ser Lys Ser Asn Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Met Val Ser Asp Phe Arg Lys Asp Phe Gly Phe Tyr Lys Leu Arg 
                965                 970                 975     


Glu Val Asn Asp Tyr His His Ala Gln Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Leu Lys Lys  Tyr Pro Lys Leu Glu  Ala Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys His  Tyr Asp Leu Ala Lys  Leu Met Ile 
    1010                 1015                 1020             


Gln Pro  Asp Ser Ser Leu Gly  Lys Ala Thr Thr Arg  Met Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Leu Met Asn Phe  Phe Lys Lys Glu Ile  Lys Leu Ala 
    1040                 1045                 1050             


Asp Asp  Thr Ile Phe Thr Arg  Pro Gln Ile Glu Val  Asn Thr Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Val Lys Asp Met  Gln Thr Ile 
    1070                 1075                 1080             


Arg Lys  Val Met Ser Tyr Pro  Gln Val Asn Ile Val  Met Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Trp Pro Lys 
    1100                 1105                 1110             


Gly Asp  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Ser  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Ile Ile Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Asn Cys  Lys Arg Lys Asn Ser  Lys Thr Lys 
    1145                 1150                 1155             


Asn Tyr  Gln Arg Thr Cys Gly  Asn Lys Asn Asn Gly  Thr Arg 
    1160                 1165                 1170         


<210>  755
<211>  1173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  755

Met Arg Pro Ile Glu Pro Trp Ile Leu Gly Leu Asp Ile Gly Thr Asp 
1               5                   10                  15      


Ser Leu Gly Trp Ala Val Phe Ser Cys Glu Glu Lys Gly Pro Pro Thr 
            20                  25                  30          


Ala Lys Glu Leu Leu Gly Gly Gly Val Arg Leu Phe Asp Ser Gly Arg 
        35                  40                  45              


Asp Ala Lys Asp His Thr Ser Arg Gln Ala Glu Arg Gly Ala Phe Arg 
    50                  55                  60                  


Arg Ala Arg Arg Gln Thr Arg Thr Trp Pro Trp Arg Arg Asp Arg Leu 
65                  70                  75                  80  


Ile Ala Leu Phe Gln Ala Ala Gly Leu Thr Pro Pro Ala Ala Glu Thr 
                85                  90                  95      


Arg Gln Ile Ala Leu Ala Leu Arg Arg Glu Ala Val Ser Arg Pro Leu 
            100                 105                 110         


Ala Pro Asp Ala Leu Trp Ala Ala Leu Leu His Leu Ala His His Arg 
        115                 120                 125             


Gly Phe Arg Ser Asn Arg Ile Asp Lys Arg Glu Arg Ala Ala Ala Lys 
    130                 135                 140                 


Ala Leu Ala Lys Ala Lys Pro Ala Lys Ala Thr Ala Lys Ala Thr Ala 
145                 150                 155                 160 


Pro Ala Lys Glu Ala Asp Asp Glu Ala Gly Phe Trp Glu Gly Ala Glu 
                165                 170                 175     


Ala Ala Leu Arg Gln Arg Met Ala Ala Ser Gly Ala Pro Thr Val Gly 
            180                 185                 190         


Ala Leu Leu Ala Asp Asp Leu Asp Arg Gly Gln Pro Val Arg Met Arg 
        195                 200                 205             


Tyr Asn Gln Ser Asp Arg Asp Gly Val Val Ala Pro Thr Arg Ala Leu 
    210                 215                 220                 


Ile Ala Glu Glu Leu Ala Glu Ile Val Ala Arg Gln Ser Ser Ala Tyr 
225                 230                 235                 240 


Pro Gly Leu Asp Trp Pro Ala Val Thr Arg Leu Val Leu Asp Gln Arg 
                245                 250                 255     


Pro Leu Arg Ser Lys Gly Ala Gly Pro Cys Ala Phe Leu Pro Gly Glu 
            260                 265                 270         


Asp Arg Ala Leu Arg Ala Leu Pro Thr Val Gln Asp Phe Ile Ile Arg 
        275                 280                 285             


Gln Thr Leu Ala Asn Leu Arg Leu Pro Ser Thr Ser Ala Asp Glu Pro 
    290                 295                 300                 


Arg Pro Leu Thr Asp Glu Glu His Ala Lys Ala Leu Ala Leu Leu Ser 
305                 310                 315                 320 


Thr Ala Arg Phe Val Glu Trp Pro Ala Leu Arg Arg Ala Leu Gly Leu 
                325                 330                 335     


Lys Arg Gly Val Lys Phe Thr Ala Glu Thr Glu Arg Asn Gly Ala Lys 
            340                 345                 350         


Gln Ala Ala Arg Gly Thr Ala Gly Asn Leu Thr Glu Ala Ile Leu Ala 
        355                 360                 365             


Pro Leu Ile Pro Gly Trp Ser Gly Trp Asp Leu Asp Arg Lys Asp Arg 
    370                 375                 380                 


Val Phe Ser Asp Leu Trp Ala Ala Arg Gln Asp Arg Ser Ala Leu Leu 
385                 390                 395                 400 


Ala Leu Ile Gly Asp Pro Arg Gly Pro Thr Arg Val Thr Glu Asp Glu 
                405                 410                 415     


Thr Ala Glu Ala Val Ala Asp Ala Ile Gln Ile Val Leu Pro Thr Gly 
            420                 425                 430         


Arg Ala Ser Leu Ser Ala Lys Ala Ala Arg Ala Ile Ala Gln Ala Met 
        435                 440                 445             


Ala Pro Gly Ile Gly Tyr Asp Glu Ala Val Thr Leu Ala Leu Gly Leu 
    450                 455                 460                 


His His Ser His Arg Pro Arg Gln Glu Arg Leu Ala Arg Leu Pro Tyr 
465                 470                 475                 480 


Tyr Ala Ala Ala Leu Pro Asp Val Gly Leu Asp Gly Asp Pro Val Gly 
                485                 490                 495     


Pro Pro Pro Ala Glu Asp Asp Gly Ala Ala Ala Glu Ala Tyr Tyr Gly 
            500                 505                 510         


Arg Ile Gly Asn Ile Ser Val His Ile Ala Leu Asn Glu Thr Arg Lys 
        515                 520                 525             


Ile Val Asn Ala Leu Leu His Arg His Gly Pro Ile Leu Arg Leu Val 
    530                 535                 540                 


Met Val Glu Thr Thr Arg Glu Leu Lys Ala Gly Ala Asp Glu Arg Lys 
545                 550                 555                 560 


Arg Met Ile Ala Glu Gln Ala Glu Arg Glu Arg Glu Asn Ala Glu Ile 
                565                 570                 575     


Asp Val Glu Leu Arg Lys Ser Asp Arg Trp Met Ala Asn Ala Arg Glu 
            580                 585                 590         


Arg Arg Gln Arg Val Arg Leu Ala Arg Arg Gln Asn Asn Leu Cys Pro 
        595                 600                 605             


Tyr Thr Ser Thr Pro Ile Gly His Ala Asp Leu Leu Gly Asp Ala Tyr 
    610                 615                 620                 


Asp Ile Asp His Val Ile Pro Leu Ala Arg Gly Gly Arg Asp Ser Leu 
625                 630                 635                 640 


Asp Asn Met Val Leu Cys Gln Ser Asp Ala Asn Lys Thr Lys Gly Asp 
                645                 650                 655     


Lys Thr Pro Trp Glu Ala Phe His Asp Lys Pro Gly Trp Ile Ala Gln 
            660                 665                 670         


Arg Asp Asp Phe Leu Ala Arg Leu Asp Pro Gln Thr Ala Lys Ala Leu 
        675                 680                 685             


Ala Trp Arg Phe Ala Asp Asp Ala Gly Glu Arg Val Ala Arg Lys Ser 
    690                 695                 700                 


Ala Glu Asp Glu Asp Gln Gly Phe Leu Pro Arg Gln Leu Thr Asp Thr 
705                 710                 715                 720 


Gly Tyr Ile Ala Arg Val Ala Leu Arg Tyr Leu Ser Leu Val Thr Asn 
                725                 730                 735     


Glu Pro Asn Ala Val Val Ala Thr Asn Gly Arg Leu Thr Gly Leu Leu 
            740                 745                 750         


Arg Leu Ala Trp Asp Ile Thr Pro Gly Pro Ala Pro Arg Asp Leu Leu 
        755                 760                 765             


Pro Thr Pro Arg Asp Ala Leu Arg Asp Asp Thr Ala Ala Arg Arg Phe 
    770                 775                 780                 


Leu Asp Gly Leu Thr Pro Pro Pro Leu Ala Lys Ala Val Glu Gly Ala 
785                 790                 795                 800 


Val Gln Ala Arg Leu Ala Ala Leu Gly Arg Ser Arg Val Ala Asp Ala 
                805                 810                 815     


Gly Leu Ala Asp Ala Leu Gly Leu Thr Leu Ala Ser Leu Gly Gly Gly 
            820                 825                 830         


Gly Lys Asn Arg Ala Asp His Arg His His Phe Ile Asp Ala Ala Met 
        835                 840                 845             


Ile Ala Val Thr Thr Arg Gly Leu Ile Asn Gln Ile Asn Gln Ala Ser 
    850                 855                 860                 


Gly Ala Gly Arg Ile Leu Asp Leu Arg Lys Trp Pro Arg Thr Asn Phe 
865                 870                 875                 880 


Glu Pro Pro Tyr Pro Thr Phe Arg Ala Glu Val Met Lys Gln Trp Asp 
                885                 890                 895     


His Ile His Pro Ser Ile Arg Pro Ala His Arg Asp Gly Gly Ser Leu 
            900                 905                 910         


His Ala Ala Thr Val Phe Gly Val Arg Asn Arg Pro Asp Ala Arg Val 
        915                 920                 925             


Leu Val Gln Arg Lys Pro Val Glu Lys Leu Phe Leu Asp Ala Asn Ala 
    930                 935                 940                 


Lys Pro Leu Pro Ala Asp Lys Ile Ala Glu Ile Ile Asp Gly Phe Ala 
945                 950                 955                 960 


Ser Pro Arg Met Ala Lys Arg Phe Lys Ala Leu Leu Ala Arg Tyr Gln 
                965                 970                 975     


Ala Ala His Pro Glu Val Pro Pro Ala Leu Ala Ala Leu Ala Val Ala 
            980                 985                 990         


Arg Asp Pro Ala Phe Gly Pro Arg  Gly Met Thr Ala Asn  Thr Val Ile 
        995                 1000                 1005             


Ala Gly  Arg Ser Asp Gly Asp  Gly Glu Asp Ala Gly  Leu Ile Thr 
    1010                 1015                 1020             


Pro Phe  Arg Ala Asn Pro Lys  Ala Ala Val Arg Thr  Met Gly Asn 
    1025                 1030                 1035             


Ala Val  Tyr Glu Val Trp Glu  Ile Gln Val Lys Gly  Arg Pro Arg 
    1040                 1045                 1050             


Trp Thr  His Arg Val Leu Thr  Arg Phe Asp Arg Thr  Gln Pro Ala 
    1055                 1060                 1065             


Pro Pro  Pro Pro Pro Glu Asn  Ala Arg Leu Val Met  Arg Leu Arg 
    1070                 1075                 1080             


Arg Gly  Asp Leu Val Tyr Trp  Pro Leu Glu Ser Gly  Asp Arg Leu 
    1085                 1090                 1095             


Phe Leu  Val Lys Lys Met Ala  Val Asp Gly Arg Leu  Ala Leu Trp 
    1100                 1105                 1110             


Pro Ala  Arg Leu Ala Thr Gly  Lys Ala Thr Ala Leu  Tyr Ala Gln 
    1115                 1120                 1125             


Leu Ser  Cys Pro Asn Ile Asn  Leu Asn Gly Asp Gln  Gly Tyr Cys 
    1130                 1135                 1140             


Val Gln  Ser Ala Glu Gly Ile  Arg Lys Glu Lys Ile  Arg Thr Thr 
    1145                 1150                 1155             


Ser Cys  Thr Ala Leu Gly Arg  Leu Arg Leu Ser Lys  Lys Ala Thr 
    1160                 1165                 1170             


<210>  756
<211>  1174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  756

Met Gly Lys Tyr Ser Ile Gly Leu Asp Ile Gly Ile Ser Ser Val Gly 
1               5                   10                  15      


Trp Ser Ile Ile Asp Ile Glu Lys Lys Lys Ile Val Asp Leu Gly Ser 
            20                  25                  30          


Arg Ile Phe Pro Ser Gly Asn Ala Ala Ala Asn Gln Asp Arg Arg Gly 
        35                  40                  45              


Tyr Arg Gly Thr Arg Arg Leu Ile Arg Arg Arg Lys Asn Arg Leu Ala 
    50                  55                  60                  


Asp Val Ile Asn Phe Leu Gly Glu Glu Asn Phe Ile Asp Thr Lys Val 
65                  70                  75                  80  


Val Asp Gln Asn Gly Asn Ile His Tyr Lys Leu Leu Val Asn Ser Thr 
                85                  90                  95      


Glu Ser Pro Tyr Lys Leu Arg Val Lys Gly Leu Ser Lys Met Leu Thr 
            100                 105                 110         


Lys Gln Glu Leu Val Ile Ala Leu Tyr His Ile Val Lys His Arg Gly 
        115                 120                 125             


Ile Ser Tyr Asp Leu Gly Asp Leu Glu Asp Asp Gly Thr Ser Gly Val 
    130                 135                 140                 


Thr Asp Tyr Lys Thr Ser Ile Asn Ile Asn Arg Gln Leu Leu Ile Lys 
145                 150                 155                 160 


Lys Thr Val Gly Gln Ile Gln Leu Glu Arg Leu Lys Glu Phe Gly Lys 
                165                 170                 175     


Val Arg Gly His Val Lys Lys Asp Glu Asn Ile Thr Leu Leu Asn Val 
            180                 185                 190         


Phe Pro Ser Ser Ala Tyr Val Lys Glu Ala Thr Gln Ile Leu Thr Lys 
        195                 200                 205             


Gln Arg Glu Phe Tyr Pro Glu Ile Thr Asp Asp Phe Ile Thr Lys Phe 
    210                 215                 220                 


Ile Pro Phe Ile Ser Arg Lys Arg Asp Tyr Phe Val Gly Pro Gly Asn 
225                 230                 235                 240 


Glu Lys Ser Arg Thr Asp Tyr Gly Ile Tyr Lys Lys Asp Gly Arg Thr 
                245                 250                 255     


Leu Asp Asn Leu Phe Asp Glu Leu Ile Gly Ile Asp Lys Ile Ser Gly 
            260                 265                 270         


Glu Lys Arg Ala Ser Ala Ser Ser Val Thr Ala Gln Ile Tyr Asn Met 
        275                 280                 285             


Leu Asn Asp Leu Asn Asn Leu Thr Val Thr Asn Thr Glu Asp Gly Lys 
    290                 295                 300                 


Leu Thr Thr Gln Thr Lys Arg Glu Ile Ile Glu Ala Asp Lys Thr Thr 
305                 310                 315                 320 


Asn Gly Leu Phe Gly Ile Thr Gln Ile Cys Lys Ile Ile Gly Cys Lys 
                325                 330                 335     


Lys Asp Asp Ile Lys Gly Phe Arg Ile Asp Lys Val Glu Lys Pro Ile 
            340                 345                 350         


Met His Thr Leu Ser Gly Tyr Arg Arg Phe Arg Lys Glu Leu Ala Lys 
        355                 360                 365             


Ile Asn Ile Glu Ala Asn Asp Leu Asp Ile Asn Leu Val Asn Lys Ile 
    370                 375                 380                 


Ala Asp Ile Leu Thr Leu Asn Thr Glu Lys Ala Glu Ile Arg Lys Gln 
385                 390                 395                 400 


Leu Asn Lys Ser Asn Ile Asp Val Ser Asn Glu Ile Lys Glu Leu Ile 
                405                 410                 415     


Ile Asn Lys Asn Lys Asp Phe Leu Val Asp Gly Arg Ala Thr Trp His 
            420                 425                 430         


Ser Phe Ser Tyr Lys Thr Leu Asn Leu Leu Ile Pro Glu Leu Met Asn 
        435                 440                 445             


Thr Ser Glu Glu Gln Ile Thr Ile Leu Thr Arg Leu Gly Leu Val Lys 
    450                 455                 460                 


Ser Asn Ser Glu Lys Tyr Lys Gly Leu Lys Asn Ile Pro Ala Arg Arg 
465                 470                 475                 480 


Ile Thr Glu Gly Ile Tyr Asn Pro Val Val Ala Lys Ala Thr Arg Glu 
                485                 490                 495     


Ala Val Asn Val Phe Asn Ala Ile Ala Lys Lys Ile Gly Arg Asp Asn 
            500                 505                 510         


Ile Ser Asp Val Val Ile Glu Leu Pro Arg Glu Asp Asn Glu Ala Asp 
        515                 520                 525             


Ile Lys Lys Gly Ile Val Lys Arg Gln Lys Gln Asn Glu Leu Glu Lys 
    530                 535                 540                 


Glu Ala Ala Asp Ala Leu Val Lys Gln Gln Met Thr Ile Ser Asp Ser 
545                 550                 555                 560 


Ala Leu Val Ala His Tyr Arg Lys Val Arg Gly Leu Ser Gln Lys Val 
                565                 570                 575     


Arg Tyr Trp Tyr Gln Gln Gly Thr Cys Cys Pro Tyr Cys Gly Lys Asn 
            580                 585                 590         


Ile Glu Ala Val Gln Leu Ile Asn Asn Asn Asp Ser Phe Glu Val Asp 
        595                 600                 605             


His Ile Ile Pro Ile Ser Val Ser Phe Asp Asp Ser Gln Asn Asn Lys 
    610                 615                 620                 


Val Leu Val His Ser Gln Cys Asn Gln Ala Lys Gly Lys Gln Thr Pro 
625                 630                 635                 640 


Leu Gly Trp Leu Asn Asn Gly Gly Gly Phe Gly Gln Asn Lys Thr Glu 
                645                 650                 655     


Tyr Val Ala Lys Val Lys Thr Asn Lys Asn Tyr Ser Lys Asn Lys Met 
            660                 665                 670         


Asp Asn Leu Leu Asn Asp Met Asp Leu Ser Asp Ile Val Thr Gln Arg 
        675                 680                 685             


Gly Phe Ile Gln Arg Asn Leu Asn Asp Thr Arg Tyr Ala Ser Arg Ile 
    690                 695                 700                 


Val Leu Asp Glu Phe Tyr Ser Phe Phe Lys Ser Asn Asn Leu Ser Thr 
705                 710                 715                 720 


Lys Val Lys Ile Val Arg Gly Lys Trp Thr Ser Gln Met Arg Lys Lys 
                725                 730                 735     


Trp Asn Gly Val Gly Gly Leu Lys Lys Thr Arg Asp Thr Tyr His His 
            740                 745                 750         


His Ala Ile Asp Ala Ser Ile Ile Ala Ser Phe Pro Leu Leu Lys Val 
        755                 760                 765             


Phe Asp Lys Ala Ile Lys Leu Ile Asp Ile Asp Arg Glu Thr Gly Glu 
    770                 775                 780                 


Ile Leu Gln Asp Lys Glu Ala Ile Lys Met Ser Gln Glu Ala Lys Leu 
785                 790                 795                 800 


Leu Glu Lys Ile Pro Val Ile Gln Asn Glu Lys Phe Glu Glu Leu Ile 
                805                 810                 815     


Asn Glu Leu Tyr Asp Phe Pro Leu Phe Lys Gln Val Glu Leu Ala Asn 
            820                 825                 830         


Asp Ile Thr Asn Pro Glu Asn Pro Val Lys Phe Ser His His Val Asp 
        835                 840                 845             


Lys Lys Ala Asn Arg Ser Val Ala Asn Gln Thr Ile Tyr Gly Thr Arg 
    850                 855                 860                 


Ile Lys Glu Lys Glu Ile Asn Lys Arg Gly Lys Val Glu Lys Val Gln 
865                 870                 875                 880 


Glu Arg Tyr Ile Leu Gly Thr Ile Lys Asn Ile Tyr Asp Ile Asp Ser 
                885                 890                 895     


Phe Thr Ser Phe Lys Lys Ile Tyr Asp Lys Met Ser Lys Thr Gly Asp 
            900                 905                 910         


Val Lys Phe Leu Met Gln Lys Lys Asp Pro Lys Thr Trp Gly Lys Leu 
        915                 920                 925             


Val Glu Ile Leu Asn Ser Tyr Pro Asp Ser Glu Glu Ile Thr Gln Val 
    930                 935                 940                 


Asp Gly Lys Val Lys Arg Val Lys Val Ser Pro Phe Glu Leu Tyr Arg 
945                 950                 955                 960 


Arg Glu Asn Asp Phe Ile Thr Lys Tyr Ala Lys His Asn Asp Gly Pro 
                965                 970                 975     


Lys Ile Val Ser Ile Lys Tyr Tyr Asp Ser Lys Ile Gly Ser His Ile 
            980                 985                 990         


Asp Ile Thr Pro Ile Asn Ala Lys  Asn Lys Val Val Leu  Gln Ser Leu 
        995                 1000                 1005             


Lys Pro  Trp Arg Thr Asp Val  Tyr Phe Asn Ser Glu  Thr Arg Gln 
    1010                 1015                 1020             


Tyr Glu  Leu Leu Gly Leu Lys  Tyr Ser Asp Leu Lys  Phe Thr Asn 
    1025                 1030                 1035             


Gly Val  Tyr Gly Ile Thr Ser  Glu Ala Tyr Glu Lys  Leu Lys His 
    1040                 1045                 1050             


Gly Ile  Ser Glu Asp Gly Thr  Lys Thr Trp Lys Pro  Ile Gly Lys 
    1055                 1060                 1065             


Asn Ser  Gly Phe Arg Phe Ser  Leu Tyr Arg Asn Asn  Arg Val Lys 
    1070                 1075                 1080             


Ile Val  Asp Glu Lys Gly Glu  Ser Leu Glu Leu Leu  Phe Ala Ser 
    1085                 1090                 1095             


Arg Thr  Thr Ser Asn Glu Gly  Tyr Val Glu Leu Lys  Pro Ile Tyr 
    1100                 1105                 1110             


Lys Ser  Lys Phe Asp Ser Lys  Glu Asp Val Gly Phe  Tyr Gly Gln 
    1115                 1120                 1125             


Met Thr  Pro Ser Gly Gln Phe  Val Lys Lys Met Ala  Arg Val Gly 
    1130                 1135                 1140             


Tyr Lys  Leu Tyr Lys Val Asn  Thr Asp Val Leu Gly  Asn Pro Tyr 
    1145                 1150                 1155             


Tyr Ile  Thr Lys Glu Ser Asp  Asp Pro Glu Leu Ser  Leu Tyr Lys 
    1160                 1165                 1170             


Lys 
    


<210>  757
<211>  1175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  757

Met Glu Glu Met Lys Gln Thr Gly Arg Lys Asn Glu Ala Pro Lys Leu 
1               5                   10                  15      


Thr Asp Leu Asn Tyr Arg Ile Gly Leu Asp Ile Gly Ile Ala Ser Val 
            20                  25                  30          


Gly Trp Ala Val Leu Glu Asn Asn Ser His Asp Glu Pro Phe Arg Ile 
        35                  40                  45              


Ile Asp Leu Gly Val Arg Ile Phe Asp Thr Ala Glu Gln Pro Lys Thr 
    50                  55                  60                  


Gly Ala Ser Leu Ala Leu Pro Arg Arg Glu Ala Arg Ser Ala Arg Arg 
65                  70                  75                  80  


Arg Ile Arg Arg Arg Arg Asp Arg Ile Glu Arg Ile Lys Lys Leu Phe 
                85                  90                  95      


Gln Ala Glu Gly Leu Ile Asn Ile Asp Glu Phe Glu Ala Arg Tyr Lys 
            100                 105                 110         


Ile Ala Gly Leu Ser Asp Val Tyr Lys Leu Arg Tyr Glu Gly Leu Asp 
        115                 120                 125             


Arg Lys Leu Thr Asp Asp Glu Leu Ala Gln Val Leu Leu His Ile Ala 
    130                 135                 140                 


Lys His Arg Gly Phe Lys Ser Thr Ser Lys Ala Glu Glu Ala Glu Asn 
145                 150                 155                 160 


Glu Asn Asp Ala Ala Lys Lys Lys Glu Ala Gly Ala Leu Lys Lys Gly 
                165                 170                 175     


Ile Ser Asn Asn Lys Lys Leu Met Glu Glu Lys Gly Tyr Arg Thr Val 
            180                 185                 190         


Gly Glu Met Met Tyr Leu Asp Glu Asn Phe Arg Thr Asp Cys Pro Trp 
        195                 200                 205             


Asn Glu Asn Gly Tyr Ile Leu Thr Pro Arg Asn Lys Ser Gly Asp Tyr 
    210                 215                 220                 


Lys His Thr Val Leu Arg Asp Met Leu Val Asp Glu Val Arg Glu Ile 
225                 230                 235                 240 


Phe Lys Ser Gln Arg Ser Leu Gly Asn Ile His Ala Ser Glu Glu Leu 
                245                 250                 255     


Glu Asn Ala Tyr Leu Asp Ile Met Leu Ser Gln Arg Ser Phe Asp Met 
            260                 265                 270         


Gly Pro Gly Ala Pro Ser Lys Tyr Ala Met Glu Gly Phe Glu Asp Arg 
        275                 280                 285             


Val Gly Leu Cys Thr Phe Glu Gly Lys Asn Gly Glu Lys Arg Ala Ala 
    290                 295                 300                 


Lys Ala Ala Tyr Thr Ser Glu Leu Phe Val Ala Leu Glu Lys Ile Asn 
305                 310                 315                 320 


His Thr Lys Ile Val Ser Asp Asp Gly Ala Thr Arg Phe Leu Thr Thr 
                325                 330                 335     


Tyr Glu Arg Lys Ser Ile Ile Glu Leu Met His Lys Gln Gln Thr Val 
            340                 345                 350         


Lys Tyr Ala Ala Val Arg Lys Ala Ile Gly Leu Ala Glu Glu Asp Lys 
        355                 360                 365             


Phe Tyr Asn Leu Asn Tyr Ser Pro Lys Ala Gly Ser Lys Lys Ser Pro 
    370                 375                 380                 


Glu Asp Thr Asp Phe Val Lys Leu Glu Asn Tyr His Lys Ile Arg Lys 
385                 390                 395                 400 


Ala Leu Gly Glu Asp Ile Val Ser Glu His Leu Pro Ala Glu Lys Ile 
                405                 410                 415     


Lys Leu Tyr Asp Ser Ile Ala Thr Ile Leu Thr Leu Phe Lys Asn Asp 
            420                 425                 430         


Asp Ser Arg Ile Lys Arg Phe Ile Glu Leu Gly Ile Gly Glu Glu Cys 
        435                 440                 445             


Phe Asp Gly Leu Leu Ser Leu Ser Pro Ser Lys Phe His Asn Leu Ser 
    450                 455                 460                 


Leu Val Ala Met Gly Lys Ile Ile Pro Phe Leu Leu Asp Gly Asn Thr 
465                 470                 475                 480 


Tyr Asp Lys Ala Cys Glu Leu Ala Gly Tyr Asp Phe Lys Ala Asp Asn 
                485                 490                 495     


Lys Gly Glu Lys Ser Val Leu Leu Lys Gly Lys Asn Ile Thr Asp Met 
            500                 505                 510         


Val Asn Asp Ile Thr Asn Pro Val Val Arg Arg Ser Val Ser Gln Thr 
        515                 520                 525             


Ile Lys Val Ile Asn Ala Ile Ile Leu Glu Tyr Gly Ser Pro Gln Ala 
    530                 535                 540                 


Val Asn Ile Glu Leu Ala Arg Glu Met Ser Lys Asn Phe Asp Glu Arg 
545                 550                 555                 560 


Arg Lys Leu Lys Ser Asp Met Glu Ser Arg Glu Lys Glu Asn Asn Asn 
                565                 570                 575     


Val Ile Lys Arg Val Lys Glu Tyr Lys Leu Asn Pro Thr Gly Gln Asp 
            580                 585                 590         


Ile Val Lys Phe Arg Leu Trp Glu Glu Gln Asp Arg Arg Cys Leu Tyr 
        595                 600                 605             


Ser Gly Glu Lys Ile Asp Ile Asn Asp Leu Phe Ser Arg Asp Gly Gly 
    610                 615                 620                 


Tyr Asp Val Asp His Ile Leu Pro Tyr Ser Ile Thr Phe Asp Asp Thr 
625                 630                 635                 640 


Tyr Arg Asn Lys Val Leu Val Lys Ala Ser Glu Asn Arg Gln Lys Gly 
                645                 650                 655     


Asn Arg Thr Pro Tyr Glu Tyr Phe Met Ala Glu Lys Asp Asp Asp Glu 
            660                 665                 670         


Trp Asn Val Tyr Glu Thr Leu Val Arg Asn Thr Val Lys Asp Tyr Lys 
        675                 680                 685             


Lys Val Leu Lys Leu Leu Lys Lys His Ile Thr Glu Asp Glu Lys Ser 
    690                 695                 700                 


Glu Phe Arg Glu Arg Asn Leu Thr Asp Thr Lys Tyr Ile Thr Arg Ile 
705                 710                 715                 720 


Ile Tyr Asn Met Ile Arg Gln Asn Leu Val Leu Lys Pro Tyr Leu Ser 
                725                 730                 735     


Leu Glu Lys Glu Arg Lys Lys Gln Val Met Ala Val Asn Gly Ser Ile 
            740                 745                 750         


Thr His Tyr Leu Ser Lys Arg Trp Gly Leu Glu Ser Lys Asp Arg Arg 
        755                 760                 765             


Thr Asp Thr His His Ala Gln Asp Ala Val Val Ile Ala Cys Thr Thr 
    770                 775                 780                 


Asp Gly Met Ile Asn Lys Ile Ser Arg Tyr Ser Lys Gly Arg Glu Leu 
785                 790                 795                 800 


Leu Tyr Ser Arg Gly Phe Lys Phe Val Asp Glu Glu Thr Gly Glu Ile 
                805                 810                 815     


Leu Ser Arg Asp Asn Phe Thr Arg Glu Glu Trp Asp Glu Lys Tyr Gly 
            820                 825                 830         


Val Lys Ile Pro Ile Pro Trp Glu His Phe Arg Asp Glu Leu Asp Ile 
        835                 840                 845             


Arg Met Ser Arg Tyr Asn Gly Thr Gly Asp Asp Ser Ile Asp Lys Thr 
    850                 855                 860                 


Thr Pro Arg Asp Phe Ile Leu Ser His Arg Asp Ala His Lys Trp Ile 
865                 870                 875                 880 


Asn Tyr Pro Glu Trp Met Phe Val Glu Gly Arg Pro Gly Arg Arg Gly 
                885                 890                 895     


Val Leu Asp Gly Ile Phe Val Ser Arg Met Pro Lys His Lys Val Ser 
            900                 905                 910         


Gly Gln Ala His Glu Glu Thr Val Arg Ser Ala Lys Tyr Tyr Asn Asp 
        915                 920                 925             


Gly Gly Ser Val Phe Glu Ser Gly Gly Tyr Val Val Thr Lys Lys Ser 
    930                 935                 940                 


Ile Gln Asp Ile Lys Leu Lys Asp Gly Glu Ile Lys Glu Phe Phe Asn 
945                 950                 955                 960 


Pro Asp Ser Asp Arg Leu Leu Tyr Asn Ala Leu Val Asp Arg Leu Asn 
                965                 970                 975     


Lys Phe Glu Asn Asp Pro Lys Lys Ala Phe Ala Glu Pro Phe Tyr Lys 
            980                 985                 990         


Pro Lys Ala Asp Gly Thr Gln Gly  Pro Ile Val Lys Lys  Ile Lys Val 
        995                 1000                 1005             


Phe Glu  Lys Gln Ser Ser Gly  Val Phe Val Arg Asp  Gly Lys Gly 
    1010                 1015                 1020             


Ile Ala  Ala Asn Gly Ala Met  Val Arg Ile Asp Val  Phe Cys Glu 
    1025                 1030                 1035             


Glu Asp  Lys Gly Lys Lys Lys  Tyr Tyr Phe Val Pro  Ile Tyr Thr 
    1040                 1045                 1050             


Ala Asp  Val Val Lys Lys Lys  Leu Pro Asp Arg Ala  Ala Thr Ala 
    1055                 1060                 1065             


Asn Lys  Leu Ile Ser Glu Trp  Arg Val Met Lys Glu  Glu Asn Phe 
    1070                 1075                 1080             


Ile Phe  Ser Leu Tyr Ser Arg  Asp Leu Ile Tyr Ile  Glu Ser Asp 
    1085                 1090                 1095             


Lys Gly  Val Gln Val Lys Asp  Ile Asp Gly Lys Ile  Ser Lys Pro 
    1100                 1105                 1110             


Val Asn  Leu Phe Ala Tyr Tyr  Leu Gly Ala Asp Thr  Ser Thr Ala 
    1115                 1120                 1125             


Ser Ile  Ser Gly Lys Leu His  Asp Ser Ser Ala Asn  Phe Arg Ser 
    1130                 1135                 1140             


Ile Gly  Ile Gln Gly Leu Arg  Arg Phe Asp Lys Cys  Gln Val Asp 
    1145                 1150                 1155             


Val Leu  Gly Asn Ile Ser Phe  Val Asn Lys Glu Lys  Arg Met Pro 
    1160                 1165                 1170             


Phe Asn  
    1175 


<210>  758
<211>  1176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  758

Met Asn Lys Val Gln Glu Asp Cys Tyr Lys Lys Ile Lys Glu Lys Leu 
1               5                   10                  15      


Ala Asp Arg Glu Tyr Arg Ile Gly Leu Asp Leu Gly Val Gly Ser Ile 
            20                  25                  30          


Gly Tyr Ala Val Val Ser Leu Lys Gly Asp Met Pro Glu Asp Val Ile 
        35                  40                  45              


Leu Ala Gly Ser Arg Ile Phe Lys Ser Ser Ile Gly Ala Ala Glu Arg 
    50                  55                  60                  


Arg His Phe Arg Ala Gln Arg Asn Thr His Arg His His Arg Glu Arg 
65                  70                  75                  80  


Met Arg Tyr Leu Trp Lys Leu Leu Ala Glu Lys Asn Leu Ala Leu Gln 
                85                  90                  95      


Leu Pro Glu Asn Leu Ser Lys Lys Glu Asn Phe Thr Asp Phe Asp Thr 
            100                 105                 110         


Ser Arg Lys Arg Phe Pro Ile Glu Val Leu Arg Asn Asp Pro Tyr Ile 
        115                 120                 125             


Leu Arg Tyr Lys Ala Leu Asp Glu Lys Leu Ser Leu Phe Gln Leu Gly 
    130                 135                 140                 


Tyr Ala Leu Tyr His Ile Ala Asn His Arg Gly Thr Ala Ser Val Arg 
145                 150                 155                 160 


Ser Phe Leu Glu Asp Asp Glu Ala Lys Val Lys Glu Asn Lys Glu Thr 
                165                 170                 175     


Ala Lys Leu Ala Lys Asn Thr Lys Lys Ile Met Tyr Glu Lys Lys Tyr 
            180                 185                 190         


Arg Thr Phe Gly Glu Tyr Leu Tyr Arg Glu Arg Ile Glu Lys Phe Arg 
        195                 200                 205             


Gln Asn Glu Arg Met Lys Val Thr Asn Lys Asn Glu Asn Lys Glu Phe 
    210                 215                 220                 


Ala Val Thr Arg Asp Ile Ile Glu Lys Glu Val Glu Met Ile Leu Gly 
225                 230                 235                 240 


Ala Gln Lys Arg Tyr Asn Thr Ala Val Leu Ala Asp Asp Tyr Ile Glu 
                245                 250                 255     


Arg Ile Lys Lys Ala Ile Phe Tyr Glu Thr Glu Lys Leu Ile Pro Glu 
            260                 265                 270         


Pro Glu Tyr Cys Pro Tyr Phe Lys Asp Asp Lys Arg Leu Pro Arg Ser 
        275                 280                 285             


His Lys Leu Asn Glu Tyr Arg Arg Ile Tyr Glu Ala Leu Asn Asn Thr 
    290                 295                 300                 


Arg Tyr Tyr Ile Pro Glu Ile Glu Glu Lys Thr Gly Glu Ile Leu Ser 
305                 310                 315                 320 


Arg Lys Glu Lys Glu Gln Leu Ser Lys Glu Glu Lys Glu Lys Leu Phe 
                325                 330                 335     


Asn Phe Leu Leu Lys Asn Lys Glu Leu Thr Glu Ala Lys Val Lys Lys 
            340                 345                 350         


Leu Leu Asn Leu Ser Glu Met Ala Lys Val Gln Leu Gln Gly Arg Asp 
        355                 360                 365             


Lys Lys Asn Gln Lys Ile Lys Gly Tyr Ala Leu Ile Thr Leu Glu Ser 
    370                 375                 380                 


Leu Pro Phe Trp Ser Lys Leu Pro Glu Gln Gln Gln Asp Glu Phe Leu 
385                 390                 395                 400 


Tyr Asp Trp Asn Lys Leu Pro Asp Glu Lys Leu Lys Asp Arg Leu Arg 
                405                 410                 415     


Asn Tyr Tyr Lys Leu Thr Asp Asp Glu Ile Asp Glu Thr Phe Gln Asn 
            420                 425                 430         


Ile Val Leu Pro Ser Asn Tyr Ala Pro Leu Gly Lys Lys Ala Met Glu 
        435                 440                 445             


Ile Leu Leu Glu Tyr Ile Lys Asn Gly Leu Ser Tyr Ile Glu Ala Leu 
    450                 455                 460                 


Asp Lys Ala Ala Asp Glu Asn Lys Ile Lys Val Glu Lys His Glu Ile 
465                 470                 475                 480 


Asn Asp Leu Leu Pro Tyr Tyr Gly Lys Ile Leu Gln Glu Ser Thr Gln 
                485                 490                 495     


Met Pro Ile Gly Lys Ala Phe Ser Lys Gln Phe Ala Ala Lys Asn Tyr 
            500                 505                 510         


Lys Lys Pro Asp Ile Asn Lys Asn Glu Gln Glu Phe Gly Arg Ile Ala 
        515                 520                 525             


Asn Pro Val Val His Gln Thr Leu Asn Glu Leu Arg Lys Leu Ile Asn 
    530                 535                 540                 


Glu Ile Ile Thr Val Phe Gly Lys Lys Pro Leu Glu Ile Ser Leu Glu 
545                 550                 555                 560 


Thr Ser Arg Glu Leu Lys Lys Ser Gln Lys Glu Arg Glu Leu Ile Ala 
                565                 570                 575     


Lys Gln Gln Asp Ile Asp Glu Glu Lys Arg Lys Lys Ile Trp Lys Thr 
            580                 585                 590         


Tyr Ile Glu Pro His Leu Gln Gln Ile Lys Ser Arg Ser Asp Lys Leu 
        595                 600                 605             


Lys Lys Leu Phe Val Phe Glu Leu Trp Glu Asp Gln Lys His Asn Cys 
    610                 615                 620                 


Pro Phe Cys Leu Glu Lys Ile Asn Val Asp Asp Ile Tyr Ser Arg Gly 
625                 630                 635                 640 


Val Asp Lys Glu His Leu Phe Pro Leu Lys Lys Ser Glu Asp Asp Ser 
                645                 650                 655     


Glu Asn Asn Leu Val Ile Ala His Asn Lys Cys Asn Ala Arg Lys Ala 
            660                 665                 670         


Glu Arg Ala Pro Phe Asp Ala Phe Gly His Glu Ser Ile Trp Asn Asp 
        675                 680                 685             


Ile Phe Ser Asn Ala Glu Lys Asn Leu Pro Arg Lys Leu Trp Arg Phe 
    690                 695                 700                 


Arg Gln Gly Ser Phe Glu Lys Phe Leu Glu Lys Pro Pro Met Leu Lys 
705                 710                 715                 720 


Arg Phe Glu Thr Asp Asn Ser Tyr Ile Ser Lys Ile Ser Ala Arg Tyr 
                725                 730                 735     


Leu Ala Cys Leu Phe Asp Lys Pro Ser Lys Ile Phe Cys Val Lys Pro 
            740                 745                 750         


Phe Leu Thr Ala Gln Leu Arg Ile Ala Trp Gln Leu Asp Glu Ile Met 
        755                 760                 765             


Ile Arg Leu Ala Glu Asn Ile Ile Leu Asp Ser Glu Lys Asp Ser Phe 
    770                 775                 780                 


Asn Lys Gly Gly Leu Gln Lys Lys Lys Arg Ile Asp Asn Arg His His 
785                 790                 795                 800 


Ala Leu Asp Ala Val Val Ile Ala Tyr Ala Ser Arg Gly Tyr His Asn 
                805                 810                 815     


Phe Leu Asn Arg Val His Ala Lys Gly Tyr Lys Ile Asn Tyr Asp Glu 
            820                 825                 830         


Lys Thr Trp Leu Ser Glu Thr Leu Arg Pro Pro Gly Asn Lys Asn Leu 
        835                 840                 845             


Glu Asp Phe Lys Ser Gly Ile Ile Asn Val Met Ile Asn Ala Asn Ile 
    850                 855                 860                 


Ser Ile Lys His Asp His Asn Asn Lys Gly Gln Leu Val Lys Ser Lys 
865                 870                 875                 880 


Ala Tyr Lys Leu Tyr Tyr Ser Glu Ile Gly Lys Tyr Ile Ile Thr Thr 
                885                 890                 895     


Leu Lys Ser Val Ser Glu Ile Leu Phe Lys Gln Lys Glu Lys Pro Gln 
            900                 905                 910         


Asp Thr Leu Arg Arg Ala Leu Cys Lys Phe Glu Asp Arg Leu Ser Asp 
        915                 920                 925             


Ile Lys Asp Lys Lys Leu Arg Lys Lys Leu Glu Asp Asn Ile Thr Ile 
    930                 935                 940                 


Tyr Lys Ser Ile Glu Asn Glu Ser Ala Leu Ser Lys Ala Lys Gln Glu 
945                 950                 955                 960 


Leu Glu Glu Phe Asp Leu Lys Ala Lys Lys Glu Gly Lys Lys Gly Tyr 
                965                 970                 975     


Glu Lys Ile Thr Asp Ile Leu Ile Tyr Lys Lys Ala Cys Ser Met His 
            980                 985                 990         


Gly Gly Lys Tyr Ile His Val Lys  Asn Arg Ile Glu Asn  Lys Phe Phe 
        995                 1000                 1005             


Ala Leu  Lys Glu Pro Thr Glu  Ile Glu Thr Gly Phe  Gly Tyr Asp 
    1010                 1015                 1020             


Thr Ala  Asp Asn Leu Ser Leu  Asp Leu Tyr His Asp  Asn Asn Gly 
    1025                 1030                 1035             


Lys Leu  Cys Gly Glu Val Ile  Arg Lys Ile Asp Phe  Asn Arg Asn 
    1040                 1045                 1050             


Arg Val  Pro Asn Tyr Lys Lys  Glu Gly Tyr Ile Leu  Leu Glu Arg 
    1055                 1060                 1065             


Ile Tyr  Gln Gly Asp Thr Leu  Glu Val Asp Ile Ser  Asp Asp Lys 
    1070                 1075                 1080             


Ile Ser  Leu Lys Asn Lys Val  Gly Ser Ala Pro Asp  Lys Arg Thr 
    1085                 1090                 1095             


Phe Val  Lys Val Leu Thr Phe  Thr Glu Ser Asp Arg  Tyr Leu Lys 
    1100                 1105                 1110             


Asn Lys  Arg Asp Gln Ile Gln  Ile Phe Phe Cys Asn  Leu Leu Lys 
    1115                 1120                 1125             


Ser Thr  Leu Lys His Asp Asp  Ser Ser Lys His Asp  Asp Ser Phe 
    1130                 1135                 1140             


Tyr Val  Ser Ser Met Gln Lys  Tyr Asn Val Arg Lys  Val Ile Leu 
    1145                 1150                 1155             


Thr Ser  Leu Gly Ile Ile Lys  Tyr Arg Ser Lys Ile  Leu Lys Asn 
    1160                 1165                 1170             


Lys Glu  Ile 
    1175     


<210>  759
<211>  1176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  759

Met Leu Phe Pro Lys Lys Leu Ser Lys Glu Glu Val Ala Tyr Val Leu 
1               5                   10                  15      


Val Glu Ile Asn Ser Asn Leu Asn Asn Ser Ser Asn Tyr Leu Gly Asn 
            20                  25                  30          


Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr Ile Gly 
        35                  40                  45              


Gln Phe Leu Tyr Thr Lys Leu Lys Glu Asp Arg His Tyr Ser Leu Lys 
    50                  55                  60                  


Asn Lys Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Arg Ile 
65                  70                  75                  80  


Trp Glu Thr Gln Ala Ile Phe His Lys Glu Leu Thr Glu Asp Leu Lys 
                85                  90                  95      


Lys Gln Ile Arg Asp Thr Ile Ile Phe Tyr Gln Arg Arg Leu Lys Ser 
            100                 105                 110         


Gln Lys Gly Leu Ile Ser Leu Cys Glu Phe Glu Asn Lys Glu Ile Thr 
        115                 120                 125             


Ile Glu Lys Glu Gly Lys Thr Val Thr Lys Gln Ile Gly Val Arg Val 
    130                 135                 140                 


Ile Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Ile 
145                 150                 155                 160 


Leu Asn Asn Val Ile Cys Lys Lys Lys Gly Thr Arg Lys Lys Lys Ala 
                165                 170                 175     


Val Thr Pro Ala Pro Leu Leu Phe Glu Leu Phe Gly Thr Pro Ser Glu 
            180                 185                 190         


Thr Phe Phe Ser Leu Asp Met Glu Cys Lys Gln Leu Leu Phe Glu Glu 
        195                 200                 205             


Leu Asn Leu Lys Gly Asn Leu Lys Ala Asp Lys Val Ile Arg Leu Leu 
    210                 215                 220                 


Gly Tyr Ile Pro Gln Glu Trp Glu Ile Asn Tyr Asn Gln Leu Glu Gly 
225                 230                 235                 240 


Asn Arg Thr Gln Lys Ser Leu Tyr Glu Ala Tyr Leu Lys Ile Ile Glu 
                245                 250                 255     


Ile Glu Gly Tyr Asp Ile Lys Thr Phe Leu Arg Ile Lys Ser Asp Lys 
            260                 265                 270         


Glu Asp Trp Asp Leu Glu Glu Ser Pro Leu Ser Ala Lys Glu Ile Arg 
        275                 280                 285             


Glu Asn Ile Gln His Ile Phe Glu Thr Leu Gly Ile Ser Thr Ala Ile 
    290                 295                 300                 


Leu Tyr Phe Asp Ala Thr Leu Pro Ile Lys Asp Phe Glu Lys Gln Ala 
305                 310                 315                 320 


Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asn Ser 
                325                 330                 335     


Pro Ser Gly Asn Glu Thr Leu Tyr Lys Leu Leu Gln Asp Lys Phe Gly 
            340                 345                 350         


Phe Lys Arg Glu His Ala Val Phe Leu Ser Asn Ile Ser Phe Gln Asp 
        355                 360                 365             


Asp Tyr Gly Ser Leu Ser Ala Lys Ala Ile Arg Lys Ile Tyr Pro Tyr 
    370                 375                 380                 


Ile Lys Glu Asn Gln Tyr Ser Thr Ala Cys Glu Leu Ala Gly Tyr Arg 
385                 390                 395                 400 


His Ser Lys Leu Ser Leu Thr Lys Glu Glu Leu Asn Asn Arg Pro Leu 
                405                 410                 415     


Lys Asp Leu Ile Ser Pro Leu Lys Lys Asn Ser Leu Arg Asn Pro Val 
            420                 425                 430         


Val Glu Lys Gly Leu Asn Gln Met Ile Asn Val Val Asn Ala Leu Ile 
        435                 440                 445             


Glu Lys Asn Ser Glu Lys Asp Ala Glu Gly Asn Ile Ile Lys Tyr Phe 
    450                 455                 460                 


His Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn 
465                 470                 475                 480 


Ala Gln Glu Arg Ala Glu Met Thr Leu Asn Ile Lys Asn Ala Thr Ile 
                485                 490                 495     


Glu Gln Glu Lys Ile Lys Ser Ile Leu Ile Ser Asp Phe Gly Ile Lys 
            500                 505                 510         


Asn Pro Thr Lys Asn Asp Ile Thr Arg Tyr Arg Leu Tyr Gln Glu Leu 
        515                 520                 525             


Ala His Asn Asp Tyr Lys Asp Leu Tyr Thr Asn Thr Asp Ile Pro Lys 
    530                 535                 540                 


Glu Leu Leu Phe Ser Lys Asn Ile Asp Ile Glu His Ile Val Pro Lys 
545                 550                 555                 560 


Ala Arg Val Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Ala Tyr Arg 
                565                 570                 575     


Asp Phe Asn Leu Lys Lys Gly Glu Asn Thr Ala Phe Asp Phe Ile Asp 
            580                 585                 590         


Ser Ser Leu Gly Glu Glu Glu Leu Asn Gln Tyr Ile Ala Arg Val Glu 
        595                 600                 605             


Asn Ala Phe Lys Thr Lys Asn Ile Ser Ala Thr Lys Tyr Arg Asn Leu 
    610                 615                 620                 


Leu Lys Lys Ala Thr Glu Ile Asn Asp Gly Phe Ile Asn Arg Asp Leu 
625                 630                 635                 640 


Arg Glu Thr Gln Tyr Ile Ala Lys Lys Ala Lys Asp Leu Leu Phe Gln 
                645                 650                 655     


Ile Thr Arg Ser Val Val Ser Thr Ser Gly Ser Ile Thr Asp Arg Leu 
            660                 665                 670         


Arg Glu Asp Trp Gly Leu Ile Asp Ile Met Lys Glu Leu Asn Met Pro 
        675                 680                 685             


Lys Tyr Arg Asp Leu Gly Leu Thr Glu Ile Ile Glu Arg Arg Asp Gly 
    690                 695                 700                 


Arg Lys Val Glu Val Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg 
705                 710                 715                 720 


His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Pro Ser Tyr 
                725                 730                 735     


Ile Gln Tyr Leu Asn His Leu Asn Ala Arg Lys Asp Glu Asn Asn Lys 
            740                 745                 750         


Leu Tyr Ala Asp Ile Leu Gly Ile Glu Arg Lys Glu Thr Thr Lys Ile 
        755                 760                 765             


His Asp Glu Asn Gly Asn Glu Lys Arg Val Phe Ile Glu Pro Ile Pro 
    770                 775                 780                 


Gly Phe Arg Gln Ile Ala Lys Lys Tyr Leu Glu Glu Ile Leu Ile Ser 
785                 790                 795                 800 


His Lys Thr Lys Asn Lys Val Val Thr Lys Ser Ile Asn Lys Ile Ala 
                805                 810                 815     


Gly Thr Asn Lys Lys Gln Glu Thr Leu Thr Pro Arg Gly Gln Leu His 
            820                 825                 830         


Lys Glu Thr Ile Tyr Gly Lys Tyr His Tyr Tyr Ser Asn Lys Glu Glu 
        835                 840                 845             


Lys Ile Gly Thr Lys Phe Asp Lys Glu Thr Ile Ser Lys Val Ser Asn 
    850                 855                 860                 


Pro Ile Tyr Arg Tyr Ala Leu Gln Lys Arg Leu Glu Ala Asn Glu Asn 
865                 870                 875                 880 


Asn Pro Lys Lys Ala Phe Thr Gly Lys Asn Ala Leu Ser Lys Asn Pro 
                885                 890                 895     


Ile Tyr Leu Asp Asp Ala Lys Thr Lys Ile Leu Pro Glu Lys Val Asn 
            900                 905                 910         


Leu Ile Tyr Leu Glu Glu Asp Phe Ser Ile Arg Lys Lys Ile Val Ser 
        915                 920                 925             


Glu Asn Pro Thr Ser Lys Glu Phe Val Pro Asn Ser Ile Ser Ile Glu 
    930                 935                 940                 


Lys Ile Val Asp Glu Gly Ile Lys Arg Ile Leu Lys Glu Arg Leu Glu 
945                 950                 955                 960 


Thr Tyr Asn Asn Asp Pro Lys Lys Ala Phe Ala Asp Leu Asp Lys Asn 
                965                 970                 975     


Pro Ile Trp Leu Asn Lys Glu Lys Gly Ile Ser Ile Lys Arg Val Thr 
            980                 985                 990         


Ile Ser Gly Val Lys Asn Thr Gln  Ala Leu His Ser Lys  Lys Asp His 
        995                 1000                 1005             


Leu Gly  Lys Pro Ile Leu Asp  Lys Glu Gly Gln Glu  Ile Pro Val 
    1010                 1015                 1020             


Asp Phe  Val Ser Thr Gly Asn  Asn His His Val Ala  Ile Tyr Arg 
    1025                 1030                 1035             


Asp Gln  Lys Gly Asn Leu Gln  Glu Gln Ile Val Ser  Phe Phe Glu 
    1040                 1045                 1050             


Ala Val  Val Arg Ala Gln Gln  Gly Leu Pro Ile Ile  Asp Lys Thr 
    1055                 1060                 1065             


Tyr Lys  Gln Glu Glu Gly Trp  Gln Phe Leu Phe Thr  Met Lys Gln 
    1070                 1075                 1080             


Asn Glu  Met Phe Val Phe Pro  Asn Glu Ile Thr Gly  Phe Asn Pro 
    1085                 1090                 1095             


Ser Glu  Ile Asp Leu Leu Asp  Pro Lys Asn Lys Lys  Leu Ile Ser 
    1100                 1105                 1110             


Pro Asn  Leu Phe Arg Val Gln  Lys Ile Ser Ile Lys  Asp Tyr Phe 
    1115                 1120                 1125             


Phe Arg  His His Leu Glu Thr  Asn Val Glu Thr Asn  Asn Ile Leu 
    1130                 1135                 1140             


Lys Asp  Ile Thr Trp Lys Arg  Gly Gly Leu Ser Leu  Leu Lys Gly 
    1145                 1150                 1155             


Ile Ile  Lys Val Arg Ile Asn  His Leu Gly Asn Ile  Val Ser Ile 
    1160                 1165                 1170             


Gly Glu  Tyr 
    1175     


<210>  760
<211>  1177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  760

Met Asn Lys Val Gln Glu Ile Glu Tyr Lys Lys Ile Lys Glu Lys Leu 
1               5                   10                  15      


Ala Asp Arg Glu Tyr Arg Ile Gly Leu Asp Leu Gly Val Gly Ser Ile 
            20                  25                  30          


Gly Tyr Ala Val Val Ser Leu Lys Lys Tyr Asp Asp Leu Ser Tyr Leu 
        35                  40                  45              


Pro Glu Asp Ile Ile Leu Ala Gly Ser Arg Ile Phe Ala Ser Ser Ile 
    50                  55                  60                  


Gly Ala Val Glu Arg Arg Glu Phe Arg Leu Gln Arg Asn Ser His Arg 
65                  70                  75                  80  


His Arg Arg Glu Arg Met Arg Phe Leu Trp Lys Leu Leu Ala Glu Lys 
                85                  90                  95      


Gln Leu Ala Leu Gln Pro Ser Lys Asp Leu Glu Lys Lys Glu Asn Ser 
            100                 105                 110         


Ala Asp Cys Glu Thr Ser Gln Lys Arg Phe Pro Ile Asn Ile Leu Lys 
        115                 120                 125             


Asn Asp Pro Tyr Ile Leu Arg Tyr Lys Ala Leu Asp Glu Lys Leu Ser 
    130                 135                 140                 


Leu Phe Gln Leu Gly Tyr Val Leu Tyr His Val Ala Asn His Arg Gly 
145                 150                 155                 160 


Thr Asp Ser Ile Arg Ser Phe Leu Glu Asp Thr Asp Asp Lys Ile Lys 
                165                 170                 175     


Glu Asn Thr Lys Thr Ala Lys Leu Ala Thr Glu Thr Lys Lys Ile Ile 
            180                 185                 190         


Asp Ser Lys Lys Tyr Arg Thr Phe Gly Glu Tyr Leu Tyr Lys Glu Arg 
        195                 200                 205             


Ile Gln Gln Asn Glu Arg Lys Lys Val Ser Asn Arg Lys Thr Ala Gly 
    210                 215                 220                 


Asn Lys Asn Lys Glu Phe Ala Val Thr Arg Asp Met Ile Phe Lys Glu 
225                 230                 235                 240 


Thr Glu Lys Ile Leu Ala Ser Gln Lys Gln Tyr Tyr Thr Asn Val Leu 
                245                 250                 255     


Thr Asp Glu Tyr Ile Lys Glu Ile Glu Gly Ala Ile Phe Tyr Gln Thr 
            260                 265                 270         


Glu Lys Leu Ile Pro Glu Ser Gly Tyr Cys Pro Tyr Phe Lys Asp Glu 
        275                 280                 285             


Lys Arg Leu Pro Cys Ser His Lys Leu Asn Glu Tyr Arg Arg Ile Tyr 
    290                 295                 300                 


Glu Ala Leu Asn Asn Ala Arg Tyr Ser Ala Pro Val Leu Asp Glu Lys 
305                 310                 315                 320 


Thr Gly Glu Ile Leu Ser Tyr Gln Glu Gly Glu Glu Phe Ser Ser Glu 
                325                 330                 335     


Glu Lys Lys Ile Leu Phe Asp Glu Val Leu Leu Lys Gly Lys Lys Leu 
            340                 345                 350         


Thr Glu Ser Glu Ile Lys Lys Leu Leu His Leu Pro Asp Gly Cys Glu 
        355                 360                 365             


Ile Gln Leu Gln Gly Arg Asp Lys Arg Thr Gln Lys Ile Lys Gly Tyr 
    370                 375                 380                 


Ala Leu Thr Ala Leu Glu Ala Leu Pro Phe Trp Ser Lys Leu Ser Gln 
385                 390                 395                 400 


Gln Gln Gln Asp Glu Phe Leu Tyr Asp Trp Asn Gly Leu Pro Glu Glu 
                405                 410                 415     


Lys Leu Lys Pro Lys Leu Ile Asp Cys Tyr Ser Leu Thr Glu Asn Glu 
            420                 425                 430         


Val Asp Glu Thr Phe Gln Lys Ile Arg Leu Pro Ser Asn Tyr Ala Pro 
        435                 440                 445             


Val Gly Lys Lys Ala Met Glu Ile Leu Leu Thr Tyr Ile Lys Asp Gly 
    450                 455                 460                 


Tyr Ser Tyr Thr Glu Ala Leu Asp Lys Ala Val Ser Glu Gly Lys Phe 
465                 470                 475                 480 


Lys Val Asp Lys Gln Thr Thr Ile Tyr Asp Ser Leu Pro Tyr Tyr Gly 
                485                 490                 495     


Lys Ile Leu Gln Glu Ser Thr Gln Lys Leu Ile Gly Lys Ala Phe Ser 
            500                 505                 510         


Lys Gln Phe Ala Lys Arg Asn Tyr Lys Lys Pro Asn Thr Asn Arg Asn 
        515                 520                 525             


Glu Arg Glu Phe Gly Lys Ile Ala Asn Pro Val Val His Gln Ser Leu 
    530                 535                 540                 


Asn Glu Leu Arg Lys Leu Ile Asn Glu Ile Ile Thr Val Phe Gly Lys 
545                 550                 555                 560 


Lys Pro Leu Glu Ile Gly Leu Glu Thr Ala Arg Glu Leu Lys Lys Ser 
                565                 570                 575     


Ser Lys Asp Arg Glu Ser Leu Val Lys Gln Gln Asn Glu Asn Glu Tyr 
            580                 585                 590         


Asn Arg Asn Lys Ile Tyr Lys Asp Tyr Val Glu Pro His Leu Ser Gln 
        595                 600                 605             


Ile Lys Ser Arg Gln Glu Asn Pro Ser Arg Tyr Ile Phe Lys Phe Glu 
    610                 615                 620                 


Leu Phe Glu Glu Gln Lys Ser Ile Cys Pro Phe Cys Leu Lys Glu Ile 
625                 630                 635                 640 


Thr Pro Asp Asp Ile Ile Asn Ser Lys Val Asp Ile Glu His Ile Phe 
                645                 650                 655     


Pro Ile Glu Glu Ser Glu Asp Asp Ala Lys Asn Asn Leu Val Ile Ala 
            660                 665                 670         


His Asn Glu Cys Asn Ala Asp Lys Gly Lys Lys Ser Pro Phe Asp Ala 
        675                 680                 685             


Phe Gly Asp Lys Thr Ser Gly Lys Tyr Val Trp Asn Asp Ile Leu His 
    690                 695                 700                 


Asn Ala Lys Lys Asn Leu Pro His Lys Ile Trp Arg Phe Tyr Gln Gly 
705                 710                 715                 720 


Ser Leu Glu Lys Phe Leu Glu Asn Lys Pro Met Leu Lys Arg Phe Gly 
                725                 730                 735     


Thr Asp Asn Ser Tyr Ile Ser Lys Ile Ala Ala Lys Tyr Leu Ala Cys 
            740                 745                 750         


Leu Phe Asn Asn Ser Ser Lys Ile Phe Cys Ile Lys Ser Ser Leu Thr 
        755                 760                 765             


Ala Gln Leu Arg Ile Ala Trp Glu Leu Asn Ser Ile Met Ile Pro Leu 
    770                 775                 780                 


Ala Lys Asn Ile Leu Ser Glu Lys Glu Leu Glu Glu Phe Lys Thr Leu 
785                 790                 795                 800 


Lys Asn Lys Lys Ile Arg Met Asp Asn Arg His His Ala Leu Asp Ala 
                805                 810                 815     


Ile Thr Ile Ala Tyr Ala Asn Arg Gly Tyr His Asn Phe Leu Asn Lys 
            820                 825                 830         


Ile His Ala Lys Gly Tyr Lys Ile Asp Tyr Arg Glu Lys Asn Trp Leu 
        835                 840                 845             


Ser Lys Ile Leu Leu Pro Pro Leu Asn Arg Asp Leu Glu Asp Phe Lys 
    850                 855                 860                 


Ile Ser Val Lys Asn Ser Ile Glu Lys Ala Asn Val Ser Ile Lys His 
865                 870                 875                 880 


Asp His Lys Thr Asn Gly Ser Leu Val Lys Gly Thr Ala Tyr Lys Val 
                885                 890                 895     


Tyr Tyr Ser Gly Thr Gly Lys Tyr Ile Ile Thr Thr Leu Lys Asn Val 
            900                 905                 910         


Glu Glu Ile Ser Phe Lys Glu Lys Glu Asp Pro Glu Asp Thr Leu Arg 
        915                 920                 925             


Arg Ala Leu Cys Gly Phe Glu Asp Ser Asn Ile Lys Asp Glu Lys Leu 
    930                 935                 940                 


Ile Lys Lys Leu Lys Tyr Asn Ser Asp Leu Tyr Lys Lys Ile Leu Asn 
945                 950                 955                 960 


Thr Lys Pro Lys Ala Lys Gln Glu Leu Glu Gln Ala Asn Gln Glu Thr 
                965                 970                 975     


Lys Glu Glu Gly Lys Lys Thr Gln Lys Ile Thr Asp Val Leu Ile Tyr 
            980                 985                 990         


Lys Lys Ala Cys Ser Leu Val Gly  Glu Gln Tyr Ile Gln  Cys Ala Ser 
        995                 1000                 1005             


Arg Leu  Glu Asn Lys Phe Phe  Ala Val Lys Glu Pro  Thr Glu Ile 
    1010                 1015                 1020             


Arg Thr  Gly Ile Gly Tyr Asp  Thr Gly Asp Asn Leu  Phe Leu Asp 
    1025                 1030                 1035             


Leu Tyr  Tyr Asp Lys Thr Arg  Lys Leu Cys Gly Glu  Ile Ile Arg 
    1040                 1045                 1050             


Lys Ile  Asp Phe Asn Gln Asn  Lys Ile Pro Asn Tyr  Gln Lys Glu 
    1055                 1060                 1065             


Gly Tyr  Ile Leu Phe Glu Arg  Ile Tyr Gln Gly Asp  Thr Leu Glu 
    1070                 1075                 1080             


Ala Asp  Thr Ser Glu Asp Lys  Ile Ser Leu Lys Asn  Lys Thr Gly 
    1085                 1090                 1095             


Ser Ala  Leu Asp Asn Arg Thr  Phe Val Lys Val Leu  Thr Phe Thr 
    1100                 1105                 1110             


Glu Ser  Asp Lys Tyr Phe Asp  Gly Lys Lys Asp Lys  Ile Gln Ile 
    1115                 1120                 1125             


Phe Phe  Gly Asn Leu Leu Lys  Ser Asn Tyr Lys Gln  Asp Asp Ser 
    1130                 1135                 1140             


Phe Tyr  Ile Ser Ser Met Gln  Lys Tyr Asn Val Arg  Lys Val Ile 
    1145                 1150                 1155             


Leu Thr  Ser Leu Gly Ile Ile  Lys Tyr Arg Ser Gln  Ile Leu Lys 
    1160                 1165                 1170             


Asn Lys  Glu Val 
    1175         


<210>  761
<211>  1179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  761

Met Ser Lys Lys Val Ser Arg Arg Tyr Glu Glu Gln Ala Gln Glu Ile 
1               5                   10                  15      


Cys Gln Arg Leu Gly Ser Arg Pro Tyr Ser Ile Gly Leu Asp Leu Gly 
            20                  25                  30          


Val Gly Ser Ile Gly Val Ala Val Ala Ala Tyr Asp Pro Ile Lys Lys 
        35                  40                  45              


Gln Pro Ser Asp Leu Val Phe Val Ser Ser Arg Ile Phe Ile Pro Ser 
    50                  55                  60                  


Thr Gly Ala Ala Glu Arg Arg Gln Lys Arg Gly Gln Arg Asn Ser Leu 
65                  70                  75                  80  


Arg His Arg Ala Asn Arg Leu Lys Phe Leu Trp Lys Leu Leu Ala Glu 
                85                  90                  95      


Arg Asn Leu Met Leu Ser Tyr Ser Glu Gln Asp Val Pro Asp Pro Ala 
            100                 105                 110         


Arg Leu Arg Phe Glu Asp Ala Val Val Arg Ala Asn Pro Tyr Glu Leu 
        115                 120                 125             


Arg Leu Lys Gly Leu Asn Glu Gln Leu Thr Leu Ser Glu Leu Gly Tyr 
    130                 135                 140                 


Ala Leu Tyr His Ile Ala Asn His Arg Gly Ser Ser Ser Val Arg Thr 
145                 150                 155                 160 


Phe Leu Asp Glu Glu Lys Ser Ser Asp Asp Lys Lys Leu Glu Glu Gln 
                165                 170                 175     


Gln Ala Met Thr Glu Gln Leu Ala Lys Glu Lys Gly Ile Ser Thr Phe 
            180                 185                 190         


Ile Glu Val Leu Thr Ala Phe Asn Thr Asn Gly Leu Ile Gly Tyr Arg 
        195                 200                 205             


Asn Ser Glu Ser Val Lys Ser Lys Gly Val Pro Val Pro Thr Arg Asp 
    210                 215                 220                 


Ile Ile Ser Asn Glu Ile Asp Val Leu Leu Gln Thr Gln Lys Gln Phe 
225                 230                 235                 240 


Tyr Gln Glu Ile Leu Ser Asp Glu Tyr Cys Asp Arg Ile Val Ser Ala 
                245                 250                 255     


Ile Leu Phe Glu Asn Glu Lys Ile Val Pro Glu Ala Gly Cys Cys Pro 
            260                 265                 270         


Tyr Phe Pro Asp Glu Lys Lys Leu Pro Arg Cys His Phe Leu Asn Glu 
        275                 280                 285             


Glu Arg Arg Leu Trp Glu Ala Ile Asn Asn Ala Arg Ile Lys Met Pro 
    290                 295                 300                 


Met Gln Glu Gly Ala Ala Lys Arg Tyr Gln Ser Ala Ser Phe Ser Asp 
305                 310                 315                 320 


Glu Gln Arg His Ile Leu Phe His Ile Ala Arg Ser Gly Thr Asp Ile 
                325                 330                 335     


Thr Pro Lys Leu Val Gln Lys Glu Phe Pro Ala Leu Lys Thr Ser Ile 
            340                 345                 350         


Ile Val Leu Gln Gly Lys Glu Lys Ala Ile Gln Lys Ile Ala Gly Phe 
        355                 360                 365             


Arg Phe Arg Arg Leu Glu Glu Lys Ser Phe Trp Lys Arg Leu Ser Glu 
    370                 375                 380                 


Glu Gln Lys Asp Asp Phe Phe Ser Ala Trp Thr Asn Thr Pro Asp Asp 
385                 390                 395                 400 


Lys Arg Leu Ser Lys Tyr Leu Met Lys His Leu Leu Leu Thr Glu Asn 
                405                 410                 415     


Glu Val Val Asp Ala Leu Lys Thr Val Ser Leu Ile Gly Asp Tyr Gly 
            420                 425                 430         


Pro Ile Gly Lys Thr Ala Thr Gln Leu Leu Met Lys His Leu Glu Asp 
        435                 440                 445             


Gly Leu Thr Tyr Thr Glu Ala Leu Glu Arg Gly Met Glu Thr Gly Glu 
    450                 455                 460                 


Phe Gln Glu Leu Ser Val Trp Glu Gln Gln Ser Leu Leu Pro Tyr Tyr 
465                 470                 475                 480 


Gly Gln Ile Leu Thr Gly Ser Thr Gln Ala Leu Met Gly Lys Tyr Trp 
                485                 490                 495     


His Ser Ala Phe Lys Glu Lys Arg Asp Ser Glu Gly Phe Phe Lys Pro 
            500                 505                 510         


Asn Thr Asn Ser Asp Glu Glu Lys Tyr Gly Arg Ile Ala Asn Pro Val 
        515                 520                 525             


Val His Gln Thr Leu Asn Glu Leu Arg Lys Leu Met Asn Glu Leu Ile 
    530                 535                 540                 


Thr Ile Leu Gly Ala Lys Pro Gln Glu Ile Thr Val Glu Leu Ala Arg 
545                 550                 555                 560 


Glu Leu Lys Val Gly Ala Glu Lys Arg Glu Asp Ile Ile Lys Gln Gln 
                565                 570                 575     


Thr Lys Gln Glu Lys Glu Ala Val Leu Ala Tyr Ser Lys Tyr Cys Glu 
            580                 585                 590         


Pro Asn Asn Leu Asp Lys Arg Tyr Ile Glu Arg Phe Arg Leu Leu Glu 
        595                 600                 605             


Asp Gln Ala Phe Val Cys Pro Tyr Cys Leu Glu His Ile Ser Val Ala 
    610                 615                 620                 


Asp Ile Ala Ala Gly Arg Ala Asp Val Asp His Ile Phe Pro Arg Asp 
625                 630                 635                 640 


Asp Thr Ala Asp Asn Ser Tyr Gly Asn Lys Val Val Ala His Arg Gln 
                645                 650                 655     


Cys Asn Asp Ile Lys Gly Lys Arg Thr Pro Tyr Ala Ala Phe Ser Asn 
            660                 665                 670         


Thr Ser Ala Trp Gly Pro Ile Met His Tyr Leu Asp Glu Thr Pro Gly 
        675                 680                 685             


Met Trp Arg Lys Arg Arg Lys Phe Glu Thr Asn Glu Glu Glu Tyr Ala 
    690                 695                 700                 


Lys Tyr Leu Gln Ser Lys Gly Phe Val Ser Arg Phe Glu Ser Asp Asn 
705                 710                 715                 720 


Ser Tyr Ile Ala Lys Ala Ala Lys Glu Tyr Leu Arg Cys Leu Phe Asn 
                725                 730                 735     


Pro Asn Asn Val Thr Ala Val Gly Ser Leu Lys Gly Met Glu Thr Ser 
            740                 745                 750         


Ile Leu Arg Lys Ala Trp Asn Leu Gln Gly Ile Asp Asp Leu Leu Gly 
        755                 760                 765             


Ser Arg His Trp Ser Lys Asp Ala Asp Thr Ser Pro Thr Met Arg Lys 
    770                 775                 780                 


Asn Arg Asp Asp Asn Arg His His Gly Leu Asp Ala Ile Val Ala Leu 
785                 790                 795                 800 


Tyr Cys Ser Arg Ser Leu Val Gln Met Ile Asn Thr Met Ser Glu Gln 
                805                 810                 815     


Gly Lys Arg Ala Val Glu Ile Glu Ala Met Ile Pro Ile Pro Gly Tyr 
            820                 825                 830         


Ala Ser Glu Pro Asn Leu Ser Phe Glu Ala Gln Arg Glu Leu Phe Arg 
        835                 840                 845             


Lys Lys Ile Leu Glu Phe Met Asp Leu His Ala Phe Val Ser Met Lys 
    850                 855                 860                 


Thr Asp Asn Asp Ala Asn Gly Ala Leu Leu Lys Asp Thr Val Tyr Ser 
865                 870                 875                 880 


Ile Leu Gly Ala Asp Thr Gln Gly Glu Asp Leu Val Phe Val Val Lys 
                885                 890                 895     


Lys Lys Ile Lys Asp Ile Gly Val Lys Ile Gly Asp Tyr Glu Glu Val 
            900                 905                 910         


Ala Ser Ala Ile Arg Gly Arg Ile Thr Asp Lys Gln Pro Lys Trp Tyr 
        915                 920                 925             


Pro Met Glu Met Lys Asp Lys Ile Glu Gln Leu Gln Ser Lys Asn Glu 
    930                 935                 940                 


Ala Ala Leu Gln Lys Tyr Lys Glu Ser Leu Val Gln Ala Ala Ala Val 
945                 950                 955                 960 


Leu Glu Glu Ser Asn Arg Lys Leu Ile Glu Ser Gly Lys Lys Pro Ile 
                965                 970                 975     


Gln Leu Ser Glu Lys Thr Ile Ser Lys Lys Ala Leu Glu Leu Val Gly 
            980                 985                 990         


Gly Tyr Tyr Tyr Leu Ile Ser Asn  Asn Lys Arg Thr Lys  Thr Phe Val 
        995                 1000                 1005             


Val Lys  Glu Pro Ser Asn Glu  Val Lys Gly Phe Ala  Phe Asp Thr 
    1010                 1015                 1020             


Gly Ser  Asn Leu Cys Leu Asp  Phe Tyr His Asp Ala  Gln Gly Lys 
    1025                 1030                 1035             


Leu Cys  Gly Glu Ile Ile Arg  Lys Ile Gln Ala Met  Asn Pro Ser 
    1040                 1045                 1050             


Tyr Lys  Pro Ala Tyr Met Lys  Gln Gly Tyr Ser Leu  Tyr Val Arg 
    1055                 1060                 1065             


Leu Tyr  Gln Gly Asp Val Cys  Glu Leu Arg Ala Ser  Asp Leu Thr 
    1070                 1075                 1080             


Glu Ala  Glu Ser Asn Leu Ala  Lys Thr Thr His Val  Arg Leu Pro 
    1085                 1090                 1095             


Asn Ala  Lys Pro Gly Arg Thr  Phe Val Ile Ile Ile  Thr Phe Thr 
    1100                 1105                 1110             


Glu Met  Gly Ser Gly Tyr Gln  Ile Tyr Phe Ser Asn  Leu Ala Lys 
    1115                 1120                 1125             


Ser Lys  Lys Gly Gln Asp Thr  Ser Phe Thr Leu Thr  Thr Ile Lys 
    1130                 1135                 1140             


Asn Tyr  Asp Val Arg Lys Val  Gln Leu Ser Ser Ala  Gly Leu Val 
    1145                 1150                 1155             


Arg Tyr  Val Ser Pro Leu Leu  Val Asp Lys Ile Glu  Lys Asp Glu 
    1160                 1165                 1170             


Val Ala  Leu Cys Gly Glu 
    1175                 


<210>  762
<211>  1188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  762

Met Lys Tyr Ile Leu Gly Leu Asp Ile Gly Val Ser Ser Val Gly Ile 
1               5                   10                  15      


Ala Val Ile Ser Glu Glu Asn Gln Lys Lys Thr Ile Glu Asn Leu Ala 
            20                  25                  30          


Val Arg Ile Val Pro Glu Asp Pro Asp Phe His Gly Lys Phe Tyr Ser 
        35                  40                  45              


Gly Asn Thr Ala Ser Lys Asn Leu Glu Arg Thr Ala Lys Arg Gly Asn 
    50                  55                  60                  


Arg Lys Asn Asn Gln Arg Phe Lys Ala Arg Arg Asp Lys Leu Tyr Gln 
65                  70                  75                  80  


Ser Leu Lys Glu Asn Asn Met Phe Pro Thr Ser Asn Leu Phe Asp Leu 
                85                  90                  95      


Ser Ala Val Glu Leu Tyr Gly Leu Arg Ala Lys Ala Val Ser Glu Gln 
            100                 105                 110         


Val Ser Leu Gln Glu Leu Gly Arg Ile Leu Ile Leu Leu Asn Gln Arg 
        115                 120                 125             


Arg Gly Phe Leu Ser Asn Arg Lys Ser Val Ser Glu Glu Asn Ser Thr 
    130                 135                 140                 


Glu Tyr Lys Glu Arg Ile Ala Gly Leu Glu Lys Ala Leu Gly Asn Lys 
145                 150                 155                 160 


Thr Ile Gly Gln Lys Leu Tyr Ser Glu Leu Gln Glu Asn Asn Asn Val 
                165                 170                 175     


Phe Glu Val Leu Leu Arg Glu Arg Thr Tyr Gln Arg Ser Ser Tyr Thr 
            180                 185                 190         


Glu Glu Phe Asp Arg Ile Trp Asp Glu Gln Lys Lys Tyr Tyr Leu Ile 
        195                 200                 205             


Leu Thr Gly Ser Ser Asn Glu Asp Asp Asn Lys Gly Thr Leu Tyr Asp 
    210                 215                 220                 


Leu Ile Arg Asn Arg Ile Ile Tyr Tyr Gln Arg Pro Leu Lys Ser Gln 
225                 230                 235                 240 


Lys Gly Leu Ile Ser Glu Cys Leu Phe Glu Lys His His Lys Ala Val 
                245                 250                 255     


Ala Lys Ser Ser Pro Tyr Phe Glu Leu Phe Arg Ile Trp Gln Lys Ile 
            260                 265                 270         


Asn Asp Leu Ser Trp Lys Thr Pro Asn Gly Glu Leu Phe Lys Pro Thr 
        275                 280                 285             


Asp Glu Gln Lys Gln Lys Leu Lys Asn Ala Leu Trp Asn Gly Asp Asn 
    290                 295                 300                 


Leu Asn Pro Lys His Lys Leu Thr Val Ser Lys Ile Lys Glu Ile Leu 
305                 310                 315                 320 


Gly Tyr Thr Ser Arg Glu Lys Ile Tyr Leu Asn Phe Thr Glu Leu Asp 
                325                 330                 335     


Gly Ser Arg Thr Tyr Ser Ile Leu Arg Asn Ala Leu Ile Glu Ala Gly 
            340                 345                 350         


Ile Glu Lys Pro Glu Gln Tyr Leu Phe Phe Asn Met Glu Thr Asn Asp 
        355                 360                 365             


Glu Lys Gly Gly Leu Phe Glu Leu Trp His Ile Thr Tyr Ser Leu Pro 
    370                 375                 380                 


Ile Glu Lys Glu Val Ile Asn Ala Leu Lys Asn Arg Phe Gly Phe Asp 
385                 390                 395                 400 


Lys Lys Gln Ala Glu Ile Ile Ala Arg Arg Val Gly Tyr Ser Ser Asp 
                405                 410                 415     


Tyr Gly Ser Leu Ser Thr Arg Ala Ile Arg Lys Leu Leu Val His Leu 
            420                 425                 430         


Glu Lys Gly Leu Gly Tyr Thr Asp Ala Cys Asp Asn Ile Gly Tyr Asp 
        435                 440                 445             


His Ser Gly Tyr Lys Thr Lys Ile Asp Leu Gln Pro Lys Leu Lys Gln 
    450                 455                 460                 


Leu Lys Arg Asn Glu Leu Arg Asn Pro Val Val Glu Gln Ile Leu Asn 
465                 470                 475                 480 


Gln Val Val Asn Ala Val Asn Leu Ile Ile Glu Lys Tyr Gly Lys Ile 
                485                 490                 495     


Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Arg Asn Asn Ala Lys 
            500                 505                 510         


Thr Arg Lys Asn Ile Ser Leu Leu Asn Ser Lys Asn Lys Lys Asn Asn 
        515                 520                 525             


Asp Ile Ile Arg Lys Lys Leu Gln Glu Glu Tyr Gly Phe Lys Phe Val 
    530                 535                 540                 


Asn Gly Arg Asp Val Thr Arg Tyr Ile Leu Trp Glu Glu Asn Ser Lys 
545                 550                 555                 560 


Glu Cys Leu Tyr Cys Asn Lys Thr Ile Asn Gly Ser Asp Met Leu Thr 
                565                 570                 575     


Gly Asn Ala Asp Ile Glu His Ile Leu Pro Lys Ser Arg Ser Phe Asn 
            580                 585                 590         


Asn Ala Met Asn Asn Tyr Ile Ile Thr His Arg Lys Cys Asn Ser Asp 
        595                 600                 605             


Lys Gly Gln Lys Thr Ala Tyr Asp Phe Met Ser Ser Lys Gly Glu Val 
    610                 615                 620                 


Ile Leu Gln Gln Tyr Ile Glu Lys Val Asn Asp Leu Tyr Lys Asp Gly 
625                 630                 635                 640 


Lys Gly Ser Ile Ser Lys Thr Lys Phe Glu Asn Leu Leu Cys Lys Gly 
                645                 650                 655     


Glu Asp Ile Pro Ser Asp Phe Val Glu Arg Met Lys Lys Asp Ser Gln 
            660                 665                 670         


Tyr Ile Ala Lys Glu Ala Val Lys Met Leu Lys Thr Val Cys Pro Asn 
        675                 680                 685             


Val Tyr Thr Thr Thr Gly Gln Val Thr Asp Phe Leu Arg Glu Glu Trp 
    690                 695                 700                 


Lys Leu Lys Asn Leu Leu Gln Glu Leu Thr Phe Glu Lys Tyr Lys Ala 
705                 710                 715                 720 


Ile Gly Gln Val Lys Glu Lys Ile Ile Lys Thr Asn Asn Gly Gln Thr 
                725                 730                 735     


Lys Thr Phe Lys Val Ile Ala Asp Trp Ser Lys Arg Asp Asp His Arg 
            740                 745                 750         


His His Ala Ile Asp Ala Leu Ile Cys Ala Leu Thr Asp Gln Lys Ile 
        755                 760                 765             


Ile Phe Lys Leu Asn Asn Leu Asn Lys Ile Tyr Gln Tyr Glu Arg Asn 
    770                 775                 780                 


Ala Leu Ser Gln Lys Glu Ile Glu Glu Ile Glu Glu Phe Leu Glu Gly 
785                 790                 795                 800 


Lys Phe Ser Ile Lys Glu Phe Ser Ala Gln Gln Gly Asn Ile Ile Glu 
                805                 810                 815     


Cys Ser Ile Pro Asn Ile Arg Lys Asp Ala Gln Lys His Leu Glu Asn 
            820                 825                 830         


Ile Leu Ile Ser Phe Lys Lys Glu Asn Ser Lys Val Leu Thr Met Asn 
        835                 840                 845             


Arg Asn Gln Pro Lys Asn Cys Lys Glu Gln Ile Thr Trp Val Pro Arg 
    850                 855                 860                 


Ala Arg Leu His Glu Asp Thr Ile Met Gly Arg Val Lys Arg Ile Pro 
865                 870                 875                 880 


Glu Lys Lys Met Lys Leu Asn Asp Lys Val Ser Phe Glu Gln Ile Lys 
                885                 890                 895     


Met Ile Val Asn Pro Glu Ile Lys Glu Ile Leu Leu Tyr Arg Leu Ala 
            900                 905                 910         


Lys Phe Gly Asp Asn Pro Lys Val Ala Phe Ala Thr Lys Ala Leu Lys 
        915                 920                 925             


Lys Glu Pro Ile Phe Tyr Arg Glu Asn Glu Leu Lys Glu Val Ser Ile 
    930                 935                 940                 


Tyr Glu Trp Val Asn Thr Lys Arg Val Ala Leu Asn Glu Asn Ile Thr 
945                 950                 955                 960 


Asp Ser Gln Ile Glu Lys Ile Ile Asp Gln Ala Ser Lys Arg Ala Val 
                965                 970                 975     


Lys Glu Arg Leu Asn Gly Gly Lys Ile Lys Glu Val Phe Lys Asn Leu 
            980                 985                 990         


Thr Glu Asn Pro Ile Gln Leu Lys  Asn Gly Met Ile Leu  Lys Asn Val 
        995                 1000                 1005             


Thr Val  Thr Asp Glu Ser Lys  Val Glu Lys Ile Arg  Asn Gly Phe 
    1010                 1015                 1020             


Ala Ile  Thr Gly Lys Asn His  His Ala Leu Val Tyr  Lys Asp Glu 
    1025                 1030                 1035             


Asn Gly  Lys Tyr Asn Asp Lys  Val Val Ser Phe Trp  Glu Ala Val 
    1040                 1045                 1050             


Ala Ile  Gly Leu Arg Asn Val  Thr Glu Tyr Gly Asn  Pro Tyr Pro 
    1055                 1060                 1065             


Ile Ile  Asn Arg Asn His Asn  Asp Glu Leu Gly Glu  Phe Tyr Phe 
    1070                 1075                 1080             


Ser Met  Gln Ile Asn Asp Leu  Phe Val Phe Asp Leu  Lys His Ser 
    1085                 1090                 1095             


Glu Asn  Pro Gln Glu Glu Asn  Glu Ile Asn Phe Phe  Asp Val Glu 
    1100                 1105                 1110             


Asn Arg  Lys Gln Ile Ser Asp  Lys Leu Phe Arg Val  Gln Lys Met 
    1115                 1120                 1125             


Ser Lys  Lys Ser Asn Gly Gln  Phe Val Ser Asp Phe  Arg His His 
    1130                 1135                 1140             


Leu Glu  Thr Ser Val Ile Arg  Asn Asp Met Ile Leu  Lys Asn Ile 
    1145                 1150                 1155             


Thr Trp  Ile Ser Ile Ser Lys  Asn Ser Asp Leu Glu  Arg Leu Thr 
    1160                 1165                 1170             


Lys Ile  Arg Val Asn His Leu  Gly Glu Ile Ile Lys  Val Gly Glu 
    1175                 1180                 1185             


<210>  763
<211>  1194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  763

Met Asp Phe Thr Ala Asp Val Ser Glu Leu Asn Glu Ser Phe Met Gln 
1               5                   10                  15      


Ile His Thr Glu Ser Lys Phe Glu Ile Asp Thr Ala Lys Val Gln Glu 
            20                  25                  30          


Ile Ser Gln Leu Leu Leu Asp Asn Lys Ile Thr Arg Met Asp Arg Gln 
        35                  40                  45              


Lys Gln Val Ala Asn Val Leu Ala Lys Asn Ser Gly Asp Lys Glu Ala 
    50                  55                  60                  


Asp Lys Ile Asn Lys Lys Ile Ala Thr Glu Phe Cys Lys Ala Val Leu 
65                  70                  75                  80  


Gly Asn Lys Ala Lys Phe Asn Val Ile Leu Gly Glu Asp Ala Val Asp 
                85                  90                  95      


Ser Asp Trp Ser Phe Lys Met Asp Asp Glu Asn Ser Asp Glu Glu Ile 
            100                 105                 110         


Glu Ile Leu Met Gly Gln Thr Asn Gly Gln Gln Gln Asn Val Leu Arg 
        115                 120                 125             


Ile Ile His Gln Trp Tyr Ser Gln Ile Thr Leu Asn Gly Ile Val Pro 
    130                 135                 140                 


Asp Gly Gln Met Ile Ser Glu Ser Met Ile Asp Lys Tyr Glu Leu His 
145                 150                 155                 160 


Arg Lys His Leu Leu Leu Leu Lys Gln Gln Ile Lys Val Thr Lys Asp 
                165                 170                 175     


Lys Lys Lys Ala Gln Gln Leu His Glu Ala Tyr Glu Gln Tyr Val His 
            180                 185                 190         


Gly Thr Gly Ala Lys Lys Lys Leu Thr Gln Asp Asp Phe Tyr Ser Lys 
        195                 200                 205             


Val Thr Lys Asn Leu Glu Thr Ser Asp Phe Ser Asp Glu Ile Lys Lys 
    210                 215                 220                 


Gln Ile Asn Ala Glu Thr Phe Met Pro Lys Gln Arg Thr Ser Ala Asn 
225                 230                 235                 240 


Gly Val Ile Pro His Gln Leu His Gln Gln Glu Leu Asp Lys Ile Ile 
                245                 250                 255     


Glu Lys Gln Ala Lys Tyr Tyr Pro Phe Leu Ala Glu Leu Asn Pro Asp 
            260                 265                 270         


Thr Lys Arg Gln Asn Ile Ala Lys Tyr Lys Leu Asp Glu Leu Ile Ala 
        275                 280                 285             


Phe Lys Ile Pro Tyr Tyr Val Gly Pro Leu Ile Thr Thr Glu Asp Gln 
    290                 295                 300                 


Lys Lys Thr Ser Gly Lys Ser Phe Ala Trp Met Val Arg Lys Glu Pro 
305                 310                 315                 320 


Gly Lys Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Lys Met Ala 
                325                 330                 335     


Ser Ala Asp Gln Phe Ile Arg Arg Met Thr Thr Lys Asp Ser Tyr Leu 
            340                 345                 350         


Ile Thr Glu Asp Val Leu Pro Ala Glu Ser Leu Ile Tyr Gln Lys Tyr 
        355                 360                 365             


Lys Val Leu Asn Glu Leu Asn Met Ile Arg Val Asn Gly Asn Lys Leu 
    370                 375                 380                 


Thr Lys His Asp Lys Gln Asn Ile Tyr Gln Gln Val Phe Glu Lys Tyr 
385                 390                 395                 400 


Lys Thr Val Asn Val Lys Lys Val Gln Asn Tyr Phe Leu Thr Asp Gly 
                405                 410                 415     


Arg Tyr Gln His Ala Pro Lys Ile Thr Gly Leu Ser Asp Asp Asn Lys 
            420                 425                 430         


Phe Asn Ser Asn Leu Ser Thr Tyr Ile Asp Phe Lys Lys Ile Phe Gln 
        435                 440                 445             


Asp Val Val Asp Asp Pro Gln Lys Gln Lys Asp Phe Glu Arg Ile Ile 
    450                 455                 460                 


Glu Trp Ser Thr Ile Phe Glu Asp Arg His Ile Tyr Glu Val Lys Leu 
465                 470                 475                 480 


Lys Glu Ile Ser Trp Leu Asn Asp Glu Gln Arg Lys Glu Leu Lys Lys 
                485                 490                 495     


Lys Arg His Gln Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Thr Gly 
            500                 505                 510         


Ile Phe Asp Glu Asn Gly Tyr Ser Ile Met Asp Gln Leu Trp Asn Thr 
        515                 520                 525             


Gln Lys Asn Phe Met Gln Ile Gln Ala Glu Glu Gly Phe Asn Lys Lys 
    530                 535                 540                 


Ile Gln Ala Glu Asn Ser Lys His Leu Glu Lys Asn Asp Phe Glu Asp 
545                 550                 555                 560 


Ile Leu Glu Asp Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
                565                 570                 575     


Gln Val Ile Lys Val Val Glu Asp Ile Gln Lys Thr Met Gly Thr Ala 
            580                 585                 590         


Pro Gln Lys Ile Ser Ile Glu Phe Thr Arg Ser Ala Asp Lys Asn Pro 
        595                 600                 605             


Arg Arg Ser Ile Glu Arg Gln Arg Gln Leu Glu Glu Ile Tyr Tyr Asn 
    610                 615                 620                 


Asp Ala Lys Glu Ile Val Ser Glu Gly Met Gln Asp Asn Leu Gln Asn 
625                 630                 635                 640 


Phe Thr Asp Asn Lys Gln Thr Leu Thr Asp Lys Tyr Phe Leu Tyr Phe 
                645                 650                 655     


Thr Gln Leu Gly Ile Asp Met Tyr Thr Gly Lys Lys Ile Asn Ile Asp 
            660                 665                 670         


Glu Ile Gly Asn Tyr Gln Ile Asp His Ile Leu Pro Gln Ala Phe Ile 
        675                 680                 685             


Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Val Ser Ser Ala Ile Asn 
    690                 695                 700                 


Asn Gly Lys Ser Asp Asn Val Pro Leu Ser Met Tyr Ala Ser Val Arg 
705                 710                 715                 720 


Ile Lys Ser Glu Val Gln Asn Lys Trp Lys Asn Phe Ala Glu His Gly 
                725                 730                 735     


Leu Ile Ser Gln Arg Lys Leu Asn Asn Leu Met Thr Asp Pro Glu Ser 
            740                 745                 750         


Ile Asp Lys Tyr Lys Ala Arg Gly Phe Ile Lys Arg Gln Leu Val Glu 
        755                 760                 765             


Thr Ser Gln Val Ile Arg Leu Val Ala Glu Ile Leu Asn Glu Lys Tyr 
    770                 775                 780                 


Gln Gly Glu Thr Glu Ile Ile Glu Val Arg Ala Lys Met Asn Ser Gln 
785                 790                 795                 800 


Leu Arg Lys Asp Phe Asp Leu Leu Lys Asn Arg Asn Val Asn Asp Tyr 
                805                 810                 815     


His His Ala Leu Asp Ala Tyr Leu Thr Thr Phe Ile Gly Asn Tyr Leu 
            820                 825                 830         


Tyr Arg Arg Tyr Pro Lys Leu Arg Ser Tyr Phe Thr Tyr Gly His Phe 
        835                 840                 845             


Lys Lys Ile Asp Asp Ala Lys Leu Lys Gln Phe Asn Phe Leu His Asp 
    850                 855                 860                 


Ile Glu Asn Asp Lys Asp Pro Lys Tyr Val Asn Asp Asn Gly Glu Leu 
865                 870                 875                 880 


Leu Trp Asp Arg Glu Lys Asp Ile Ala Tyr Phe Lys Lys Ile Tyr Asn 
                885                 890                 895     


Phe Lys Tyr Met Leu Val Ser His Glu Val Ser Thr Arg Gln Gly Ala 
            900                 905                 910         


Met Phe Lys Gln Thr Ile Phe Pro Ala Ala Ala Asp Ala Lys Ser Lys 
        915                 920                 925             


Leu Ile Pro Ile Lys Asn Asp Lys Pro Thr Ala Ile Tyr Gly Gly Tyr 
    930                 935                 940                 


Thr Asn Asn Asn Asp Ala Tyr Leu Ala Ile Ile Lys Leu Ala Lys Thr 
945                 950                 955                 960 


Gly Gln Phe Lys Val Val Gly Val Pro Gln Arg Ala Ile Val Lys Leu 
                965                 970                 975     


Lys Gln Ala Tyr Asp Lys Glu Thr Gly Asp Tyr Ser Asn Val Leu Tyr 
            980                 985                 990         


Lys Val Leu Glu Pro Lys Phe Val  Lys Lys Lys Val Asn  Arg Lys Thr 
        995                 1000                 1005             


Gly Glu  Ile Thr Tyr Gln Val  Glu Lys Phe Lys Ile  Val Val Pro 
    1010                 1015                 1020             


Lys Val  Pro Tyr Arg Gln Leu  Val Ile Asp Gly Asp  Gln Lys Phe 
    1025                 1030                 1035             


Met Leu  Gly Ser Ser Thr Tyr  Lys Tyr Asn Ala Lys  Gln Leu Val 
    1040                 1045                 1050             


Leu Ser  Glu Asn Ala Ile Lys  Ile Leu Asp Lys Lys  Ser Phe Asp 
    1055                 1060                 1065             


Gln Ser  Gly Tyr Lys Ser Ser  Asp Leu Asp Thr Val  Tyr Asp Glu 
    1070                 1075                 1080             


Ile Leu  Ser Gln Val Asp Arg  Tyr Phe Ser Met Tyr  Asp Lys Asn 
    1085                 1090                 1095             


Lys Phe  Arg Glu Gly Leu His  Asn Gly Lys Lys Tyr  Phe Gln Arg 
    1100                 1105                 1110             


Leu Pro  Met Ile Asp Asn Val  Glu Lys Gly Lys Ile  Lys Glu Asn 
    1115                 1120                 1125             


Gly Lys  Phe Lys Leu Leu Asn  Glu Ile Leu Ile Gly  Leu His Ala 
    1130                 1135                 1140             


Asn Pro  Ser Phe Gly Asn Leu  Lys Ser Leu Gly Ile  Ser Thr Pro 
    1145                 1150                 1155             


Phe Gly  Met Met Gln Phe Ala  Ser Gly Ile Ser Leu  Ser Glu Asn 
    1160                 1165                 1170             


Ala Ile  Leu Cys Tyr Gln Ser  Pro Thr Gly Ile Phe  Glu Arg Arg 
    1175                 1180                 1185             


Val Ala  Leu Lys Asp Leu 
    1190                 


<210>  764
<211>  1195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  764

Met Gln Lys Asn Ile Asn Thr Lys Gln Asn His Ile Tyr Ile Lys Gln 
1               5                   10                  15      


Ala Gln Lys Ile Lys Glu Lys Leu Gly Asp Lys Pro Tyr Arg Ile Gly 
            20                  25                  30          


Leu Asp Leu Gly Val Gly Ser Ile Gly Phe Ala Ile Val Ser Met Glu 
        35                  40                  45              


Glu Asn Asp Gly Asn Val Leu Leu Pro Lys Glu Ile Ile Met Val Gly 
    50                  55                  60                  


Ser Arg Ile Phe Lys Ala Ser Ala Gly Ala Ala Asp Arg Lys Leu Ser 
65                  70                  75                  80  


Arg Gly Gln Arg Asn Asn His Arg His Thr Arg Glu Arg Met Arg Tyr 
                85                  90                  95      


Leu Trp Lys Val Leu Ala Glu Gln Lys Leu Ala Leu Pro Val Pro Ala 
            100                 105                 110         


Asp Leu Asp Arg Lys Glu Asn Ser Ser Glu Gly Glu Thr Ser Ala Lys 
        115                 120                 125             


Arg Phe Leu Gly Asp Val Leu Gln Lys Asp Ile Tyr Glu Leu Arg Val 
    130                 135                 140                 


Lys Ser Leu Asp Glu Arg Leu Ser Leu Gln Glu Leu Gly Tyr Val Leu 
145                 150                 155                 160 


Tyr His Ile Ala Gly His Arg Gly Ser Ser Ala Ile Arg Thr Phe Glu 
                165                 170                 175     


Asn Asp Ser Glu Glu Ala Gln Lys Glu Asn Thr Glu Asn Lys Lys Ile 
            180                 185                 190         


Ala Gly Asn Ile Lys Arg Leu Met Ala Lys Lys Asn Tyr Arg Thr Tyr 
        195                 200                 205             


Gly Glu Tyr Leu Tyr Lys Glu Phe Phe Glu Asn Lys Glu Lys His Lys 
    210                 215                 220                 


Arg Glu Lys Ile Ser Asn Ala Ala Asn Asn His Lys Phe Ser Pro Thr 
225                 230                 235                 240 


Arg Asp Leu Val Ile Lys Glu Ala Glu Ala Ile Leu Lys Lys Gln Ala 
                245                 250                 255     


Gly Lys Asp Gly Phe His Lys Glu Leu Thr Glu Glu Tyr Ile Glu Lys 
            260                 265                 270         


Leu Thr Lys Ala Ile Gly Tyr Glu Ser Glu Lys Leu Ile Pro Glu Ser 
        275                 280                 285             


Gly Phe Cys Pro Tyr Leu Lys Asp Glu Lys Arg Leu Pro Ala Ser His 
    290                 295                 300                 


Lys Leu Asn Glu Glu Arg Arg Leu Trp Glu Thr Leu Asn Asn Ala Arg 
305                 310                 315                 320 


Tyr Ser Asp Pro Ile Val Asp Ile Val Thr Gly Glu Ile Thr Gly Tyr 
                325                 330                 335     


Tyr Glu Lys Gln Phe Thr Lys Glu Gln Lys Gln Lys Leu Phe Asp Tyr 
            340                 345                 350         


Leu Leu Thr Gly Ser Glu Leu Thr Pro Ala Gln Thr Lys Lys Leu Leu 
        355                 360                 365             


Gly Leu Lys Asn Thr Asn Phe Glu Asp Ile Ile Leu Gln Gly Arg Asp 
    370                 375                 380                 


Lys Lys Ala Gln Lys Ile Lys Gly Tyr Lys Leu Ile Lys Leu Glu Ser 
385                 390                 395                 400 


Met Pro Phe Trp Ala Arg Leu Ser Glu Ala Gln Gln Asp Ser Phe Leu 
                405                 410                 415     


Tyr Asp Trp Asn Ser Cys Pro Asp Glu Lys Leu Leu Thr Glu Lys Leu 
            420                 425                 430         


Ser Asn Glu Tyr His Leu Thr Glu Glu Glu Ile Asp Asn Ala Phe Asn 
        435                 440                 445             


Glu Ile Val Leu Ser Ser Ser Tyr Ala Pro Leu Gly Lys Ser Ala Met 
    450                 455                 460                 


Leu Ile Ile Leu Glu Lys Ile Lys Asn Asp Leu Ser Tyr Thr Glu Ala 
465                 470                 475                 480 


Val Glu Glu Ala Leu Lys Glu Gly Lys Leu Thr Lys Glu Lys Gln Ala 
                485                 490                 495     


Ile Lys Asp Arg Leu Pro Tyr Tyr Gly Ala Val Leu Gln Glu Ser Thr 
            500                 505                 510         


Gln Lys Ile Ile Ala Lys Gly Phe Ser Pro Gln Phe Lys Asp Lys Gly 
        515                 520                 525             


Tyr Lys Thr Pro His Thr Asn Lys Tyr Glu Leu Glu Tyr Gly Arg Ile 
    530                 535                 540                 


Ala Asn Pro Val Val His Gln Thr Leu Asn Glu Leu Arg Lys Leu Val 
545                 550                 555                 560 


Asn Glu Ile Ile Asp Ile Leu Gly Lys Lys Pro Cys Glu Ile Gly Leu 
                565                 570                 575     


Glu Thr Ala Arg Glu Leu Lys Lys Ser Ala Glu Asp Arg Ser Lys Leu 
            580                 585                 590         


Ser Arg Glu Gln Asn Asp Asn Glu Ser Asn Arg Asn Arg Ile Tyr Glu 
        595                 600                 605             


Ile Tyr Ile Arg Pro Gln Gln Gln Val Ile Ile Thr Arg Arg Glu Asn 
    610                 615                 620                 


Pro Arg Asn Tyr Ile Leu Lys Phe Glu Leu Leu Glu Glu Gln Lys Ser 
625                 630                 635                 640 


Gln Cys Pro Phe Cys Gly Gly Gln Ile Ser Pro Asn Asp Ile Ile Asn 
                645                 650                 655     


Asn Gln Ala Asp Ile Glu His Leu Phe Pro Ile Ala Glu Ser Glu Asp 
            660                 665                 670         


Asn Gly Arg Asn Asn Leu Val Ile Ser His Ser Ala Cys Asn Ala Asp 
        675                 680                 685             


Lys Ala Lys Arg Ser Pro Trp Ala Ala Phe Ala Ser Ala Ala Lys Asp 
    690                 695                 700                 


Ser Lys Tyr Asp Tyr Asn Arg Ile Leu Ser Asn Val Lys Glu Asn Ile 
705                 710                 715                 720 


Pro His Lys Ala Trp Arg Phe Asn Gln Gly Ala Phe Glu Lys Phe Ile 
                725                 730                 735     


Glu Asn Lys Pro Met Ala Ala Arg Phe Lys Thr Asp Asn Ser Tyr Ile 
            740                 745                 750         


Ser Lys Val Ala His Lys Tyr Leu Ala Cys Leu Phe Glu Lys Pro Asn 
        755                 760                 765             


Ile Ile Cys Val Lys Gly Ser Leu Thr Ala Gln Leu Arg Met Ala Trp 
    770                 775                 780                 


Gly Leu Gln Gly Leu Met Ile Pro Phe Ala Lys Gln Leu Ile Thr Glu 
785                 790                 795                 800 


Lys Glu Ser Glu Ser Phe Asn Lys Asp Val Asn Ser Asn Lys Lys Ile 
                805                 810                 815     


Arg Leu Asp Asn Arg His His Ala Leu Asp Ala Ile Val Ile Ala Tyr 
            820                 825                 830         


Ala Ser Arg Gly Tyr Gly Asn Leu Leu Asn Lys Met Ala Gly Lys Asp 
        835                 840                 845             


Tyr Lys Ile Asn Tyr Ser Glu Arg Asn Trp Leu Ser Lys Ile Leu Leu 
    850                 855                 860                 


Pro Pro Asn Asn Ile Val Trp Glu Asn Ile Asp Ala Asp Leu Glu Ser 
865                 870                 875                 880 


Phe Glu Ser Ser Val Lys Thr Ala Leu Lys Asn Ala Phe Ile Ser Val 
                885                 890                 895     


Lys His Asp His Ser Asp Asn Gly Glu Leu Val Lys Gly Thr Met Tyr 
            900                 905                 910         


Lys Ile Phe Tyr Ser Glu Arg Gly Tyr Thr Leu Thr Thr Tyr Lys Lys 
        915                 920                 925             


Leu Ser Ala Leu Lys Leu Thr Asp Pro Gln Lys Lys Lys Thr Pro Lys 
    930                 935                 940                 


Asp Phe Leu Glu Thr Ala Leu Leu Lys Phe Lys Gly Arg Glu Ser Glu 
945                 950                 955                 960 


Met Lys Asn Glu Lys Ile Lys Ser Ala Ile Glu Asn Asn Lys Arg Leu 
                965                 970                 975     


Phe Asp Val Ile Gln Asp Asn Leu Glu Lys Ala Lys Lys Leu Leu Glu 
            980                 985                 990         


Glu Glu Asn Glu Lys Ser Lys Ala  Glu Gly Lys Lys Glu  Lys Asn Ile 
        995                 1000                 1005             


Asn Asp  Ala Ser Ile Tyr Gln  Lys Ala Ile Ser Leu  Ser Gly Asp 
    1010                 1015                 1020             


Lys Tyr  Val Gln Leu Ser Lys  Lys Glu Pro Gly Lys  Phe Phe Ala 
    1025                 1030                 1035             


Ile Ser  Lys Pro Thr Pro Thr  Thr Thr Gly Tyr Gly  Tyr Asp Thr 
    1040                 1045                 1050             


Gly Asp  Ser Leu Cys Val Asp  Leu Tyr Tyr Asp Asn  Lys Gly Lys 
    1055                 1060                 1065             


Leu Cys  Gly Glu Ile Ile Arg  Lys Ile Asp Ala Gln  Gln Lys Asn 
    1070                 1075                 1080             


Pro Leu  Lys Tyr Lys Glu Gln  Gly Phe Thr Leu Phe  Glu Arg Ile 
    1085                 1090                 1095             


Tyr Gly  Gly Asp Ile Leu Glu  Val Asp Phe Asp Ile  His Ser Asp 
    1100                 1105                 1110             


Lys Asn  Ser Phe Arg Asn Asn  Thr Gly Ser Ala Pro  Glu Asn Arg 
    1115                 1120                 1125             


Val Phe  Ile Lys Val Gly Thr  Phe Thr Glu Ile Thr  Asn Asn Asn 
    1130                 1135                 1140             


Ile Gln  Ile Trp Phe Gly Asn  Ile Ile Lys Ser Thr  Gly Gly Gln 
    1145                 1150                 1155             


Asp Asp  Ser Phe Thr Ile Asn  Ser Met Gln Gln Tyr  Asn Pro Arg 
    1160                 1165                 1170             


Lys Leu  Ile Leu Ser Ser Cys  Gly Phe Ile Lys Tyr  Arg Ser Pro 
    1175                 1180                 1185             


Ile Leu  Lys Asn Lys Glu Gly  
    1190                 1195 


<210>  765
<211>  1197
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  765

Met Ser Lys His Val Leu Gly Leu Asp Leu Gly Val Gly Ser Ile Gly 
1               5                   10                  15      


Trp Cys Leu Ile Ala Leu Asp Glu Gln Asp Gln Pro Ala Glu Ile Leu 
            20                  25                  30          


Gly Met Gly Ser Arg Ile Val Pro Leu Thr Thr Asp Asp Leu Asn Glu 
        35                  40                  45              


Phe Ser Gln Gly Lys Ala Ile Ser Lys Asn Gln Lys Arg Thr Thr Gln 
    50                  55                  60                  


Arg Thr Ile Arg Lys Gly Leu Asp Arg Tyr Gln Leu Arg Arg Lys Val 
65                  70                  75                  80  


Leu Ser Glu Lys Leu Ala Gly His Gly Met Leu Pro Asp Lys Asp Leu 
                85                  90                  95      


Met Gln Leu Pro Val Leu Asp Leu Trp Glu Leu Arg Ala Arg Ala Ala 
            100                 105                 110         


Ser Pro Gly Glu Lys Leu Ser Leu Pro Glu Ile Gly Arg Val Leu Tyr 
        115                 120                 125             


His Ile Asn Gln Lys Arg Gly Tyr Lys His Ala Lys Ser Asp Glu Ala 
    130                 135                 140                 


Glu Glu Val Thr Lys Arg Gly Lys Glu Gln Gly Tyr Val Ala Thr Val 
145                 150                 155                 160 


Lys Gly Arg Tyr Ala Glu Leu Gln Arg Glu Gly Ala Thr Val Gly Gln 
                165                 170                 175     


His Phe Ala Cys Lys Leu Lys Glu His Arg Gln Glu Ser Pro Ser Gly 
            180                 185                 190         


Gly Phe Phe Tyr Asn Tyr Arg Ile Lys Asp Gln Val Tyr Pro Arg Glu 
        195                 200                 205             


Ala Tyr Ile Glu Glu Phe Asp Arg Ile Met Glu Val Gln Ser Val Phe 
    210                 215                 220                 


Tyr Pro Glu Val Leu Thr Pro Ala Phe Ile Gln Glu Leu Arg Asp Lys 
225                 230                 235                 240 


Val Ile Phe Phe Gln Arg Pro Leu Lys Ser Cys Lys Gly Leu Val Ser 
                245                 250                 255     


Phe Cys Glu Phe Glu Lys Gln Glu Arg Val Val Gln Leu Lys Arg Val 
            260                 265                 270         


Asn Arg Glu Gly Lys Glu Glu Leu Gln Glu Arg Lys Ile Thr Ile Gly 
        275                 280                 285             


Pro Lys Val Ala Pro Lys Ser Ser Pro Ile Ala Gln Leu Cys Lys Leu 
    290                 295                 300                 


Trp Glu Ser Ile His Asn Ile Arg Ile Tyr Tyr Pro Asp Gly Lys Glu 
305                 310                 315                 320 


Arg Pro Ile Thr Leu Glu Glu Lys Arg Lys Leu Leu Ala Tyr Leu Gln 
                325                 330                 335     


Thr His Asp Lys Leu Thr Phe Thr Ala Leu Lys Lys Glu Leu Lys Ile 
            340                 345                 350         


Ser Asp Lys Glu Arg Leu Trp Cys Asp Lys Leu Leu Lys Gly Gly Ile 
        355                 360                 365             


Lys Gly Asp Thr Thr Arg Val Leu Leu Arg Lys Ala Leu Ala Gly Tyr 
    370                 375                 380                 


Lys Arg Tyr Glu Pro Leu Leu Arg Met Asp Leu Glu Leu Arg Asp Phe 
385                 390                 395                 400 


Gln Glu Ile Val Ser Gly Lys Arg Leu Ile Arg Pro Ile Val Asp Glu 
                405                 410                 415     


Ser Tyr Leu Ser Gln Pro Leu Tyr Arg Leu Trp His Ile Leu Tyr Ser 
            420                 425                 430         


Ile Glu Glu Arg Glu Ala Met Arg Arg Ala Leu Ile Asp Gln Leu Gly 
        435                 440                 445             


Phe Leu Glu Glu Asp Leu Asp Gly Gly Leu Leu Asp Lys Leu Tyr Gln 
    450                 455                 460                 


Ile Asp Phe Ala Lys Gln Gly Tyr Ser Asn Lys Ser Ala Lys Phe Ile 
465                 470                 475                 480 


Cys Lys Leu Leu Pro His Leu Gln Gly Gly His Gly Tyr Ser Gln Ala 
                485                 490                 495     


Cys Glu Leu Ala Gly Lys Arg His Ser Ala Gly Ser Leu Thr Lys Glu 
            500                 505                 510         


Glu Leu Glu Glu Arg Pro Leu Leu Glu His Ile Pro Leu Leu Gln Cys 
        515                 520                 525             


Asn Glu Leu Arg Gln Pro Ile Val Glu Lys Ile Leu Asn Gln Met Ile 
    530                 535                 540                 


Asn Leu Val Asn Ala Leu Arg Gln Lys Tyr Gly Ser Ile Asp Glu Val 
545                 550                 555                 560 


Arg Val Glu Leu Ala Arg Glu Leu Lys Met Ser Arg Glu Glu Arg Glu 
                565                 570                 575     


Glu Thr Ser Lys Arg Ile Arg Ser Arg Glu Lys Glu Asn Lys Glu Ile 
            580                 585                 590         


Ala Glu Arg Ile Lys Glu Tyr Glu Leu Tyr Pro Thr Lys Ser Arg Ile 
        595                 600                 605             


Gln Lys Tyr Arg Leu Trp Gln Glu Ala Gly Glu Gln Cys Leu Tyr Cys 
    610                 615                 620                 


Gly Arg Ser Ile Asp Val Val Gln Phe Leu Arg Gly Asp Gly Val Glu 
625                 630                 635                 640 


Ile Glu His Ile Ile Pro Lys Ser Ile Leu Tyr Asp Asp Ser Ser Ala 
                645                 650                 655     


Asn Lys Thr Cys Ala Cys Ser Arg Cys Asn Lys Asp Lys Gly Asn Arg 
            660                 665                 670         


Thr Ala Tyr Glu Tyr Ile Leu Ala Glu Gly Arg Glu Ala Glu Tyr Leu 
        675                 680                 685             


Asp Arg Leu Glu Thr Leu Phe His Asp Gly Lys Leu Ser Tyr Ser Lys 
    690                 695                 700                 


Arg Arg Arg Leu Gln Trp Lys Lys Ala Asp Ile Pro Val Asp Phe Ile 
705                 710                 715                 720 


Glu Arg Gln Leu Arg Leu Thr Gln Tyr Ile Ala Arg Gln Ala Gln Glu 
                725                 730                 735     


Ile Leu Arg Gln Gly Ile Arg Arg Val Gly Ala Ser Glu Gly Ser Val 
            740                 745                 750         


Thr Ala Lys Leu Arg His Leu Trp Gly Tyr Asp Asp Ile Leu Arg Glu 
        755                 760                 765             


Leu Asn Phe Glu Arg Tyr Gln Ser Met Gly Glu Thr Glu Ile Val Ser 
    770                 775                 780                 


Gln Glu Glu Lys Asp Gly Ser Ile Thr Lys Ser Glu Arg Ile Lys Asp 
785                 790                 795                 800 


Trp Ser Lys Arg Lys Asp His Arg His His Ala Ile Asp Ala Leu Val 
                805                 810                 815     


Val Ala Cys Thr Arg Gln Gly Tyr Ile Gln Arg Leu Asn Ser Leu Asn 
            820                 825                 830         


Ser Glu Glu Asp Ser Leu Gln Met Gln Glu Asp Ser Lys Gln Met Arg 
        835                 840                 845             


Glu Lys Met Gly Gly Gln Lys Arg Pro Thr Ala Asp Lys His Ser Ile 
    850                 855                 860                 


Leu Glu Asn Trp Leu Ile Leu Gln Pro His Phe Ser His Gln Glu Val 
865                 870                 875                 880 


Lys Ser Lys Val Ala Glu Ile Leu Val Ser Phe Arg Pro Gly Lys Arg 
                885                 890                 895     


Thr Val Ser Thr Gly Arg Asn Thr Tyr Leu Arg Ser Gly Lys Lys Val 
            900                 905                 910         


Thr Gln Ser Gly Val Leu Ile Pro Arg Gly Pro Leu Ser Glu Glu Ser 
        915                 920                 925             


Leu Tyr Gly Gln Ile Leu Arg Asn Gly Glu Pro Thr Tyr Val Lys Lys 
    930                 935                 940                 


Val Ala Leu Glu Ser Leu Ser Glu Ala Gln Ile Glu Gln Ile Val Asp 
945                 950                 955                 960 


Pro Lys Leu Arg Arg Glu Met Val Ala Arg Thr Gln Pro Ser Glu Gly 
                965                 970                 975     


Ala Lys Leu Asn Pro Lys Leu Leu Leu Ala Thr Pro Phe Tyr Ser Asp 
            980                 985                 990         


Ala Ala Gly Thr Gln Arg Val Arg  Ser Val Arg Val Ala  Ile Glu Asn 
        995                 1000                 1005             


Gly Lys  Asn Ser Ile Val Pro  Leu Arg Tyr Asn Glu  Ser Gly Lys 
    1010                 1015                 1020             


Ala Ile  Thr Phe Val Asn Pro  Gly Asn Asn His His  Val Gly Ile 
    1025                 1030                 1035             


Tyr Arg  Thr Ser Glu Gly Lys  Leu Val Glu Ser Cys  Val Thr Phe 
    1040                 1045                 1050             


Trp Gln  Ala Val Asp Arg Val  Arg Tyr Gly Ile Pro  Thr Ile Ile 
    1055                 1060                 1065             


Arg Lys  Pro Arg Glu Val Leu  Asp Gln Leu Leu Leu  Arg Asp Asp 
    1070                 1075                 1080             


Ile Pro  Glu Ala Leu Leu Ala  His Leu Pro Ala Ser  Asp Trp Glu 
    1085                 1090                 1095             


Phe Val  Glu Tyr Leu Gln Ala  Asn Glu Met Phe Leu  Ile Gly Leu 
    1100                 1105                 1110             


Thr Asp  Glu Glu Ile Gln Thr  Ala Leu Ala Glu Ser  Asp Leu Leu 
    1115                 1120                 1125             


Thr Leu  Ser Asn His Leu Tyr  Arg Val Gln Lys Met  Ser Ser Glu 
    1130                 1135                 1140             


Tyr Tyr  Val Phe Arg Tyr His  Leu Glu Thr Glu Val  Ala Asp Asn 
    1145                 1150                 1155             


Lys Asn  Asn Thr Gly Ile Ile  Pro Lys Phe Tyr Arg  Leu Arg Gly 
    1160                 1165                 1170             


Pro Asn  Asp Thr Tyr Leu Lys  Ala Asn Pro Arg Lys  Val Lys Val 
    1175                 1180                 1185             


Asn Leu  Leu Gly Glu Ile Ser  Leu Pro 
    1190                 1195         


<210>  766
<211>  1204
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  766

Met Glu Arg Ile Leu Gly Ile Asp Thr Gly Thr Asn Ser Leu Gly Trp 
1               5                   10                  15      


Ala Ile Val Asp Lys Asp Glu Asn Gln Tyr His Leu Val Ala Lys Gly 
            20                  25                  30          


Thr Asn Ile Phe Ser Glu Gly Val Lys Ile Glu Lys Gly Ile Glu Ser 
        35                  40                  45              


Ser Lys Ala Ala Glu Arg Thr Glu His Arg Ser Val Arg Lys His Tyr 
    50                  55                  60                  


Trp Arg Arg Lys Ile Arg Lys Ile Arg Leu Leu Thr Ile Leu Ser Asp 
65                  70                  75                  80  


Asn Asp Leu Cys Pro Arg Leu Lys His Glu Gln Leu Arg Gln Trp Arg 
                85                  90                  95      


Leu Lys Lys Val Tyr Pro Thr Asp Asp Thr Phe Met Glu Trp Gln Arg 
            100                 105                 110         


Thr Glu Asp Lys Ala Gln Val Asn Pro Tyr Lys Phe Arg Tyr Ile Cys 
        115                 120                 125             


Leu Thr Gln Lys Leu Asp Leu Ser Asp Ile Thr Gln Arg Phe Ile Leu 
    130                 135                 140                 


Gly Arg Ala Leu Tyr His Leu Asn Gln Arg Arg Gly Phe Leu Ser Asn 
145                 150                 155                 160 


Arg Lys Glu Ala Thr Lys Glu Ser Glu Gly Thr Val Lys Glu Gly Ile 
                165                 170                 175     


Ser Ser Leu Ser Lys Asp Met Lys Asp Ser Gly Cys Glu Tyr Leu Gly 
            180                 185                 190         


Glu Tyr Phe Tyr Gln Leu Tyr Gln Gln Gly Lys Lys Ile Arg Asn His 
        195                 200                 205             


Tyr Thr Ala Arg Lys Glu His Tyr Leu Lys Glu Phe Lys Ala Ile Cys 
    210                 215                 220                 


Ser Lys Gln Glu Leu Asp Asp Ala Leu Ile Glu Lys Leu Glu Lys Ala 
225                 230                 235                 240 


Ile Phe Asp Gln Arg Pro Leu Lys Ser Gln Lys Gly Gln Val Gly Thr 
                245                 250                 255     


Cys Thr Phe Glu Lys Gly Lys Ser Arg Cys Pro Ser Ser His Pro Leu 
            260                 265                 270         


Tyr Glu Asp Phe Arg Met Tyr Ser Phe Ile Asn Asn Ile Lys Val Gln 
        275                 280                 285             


Val Asp Thr Leu Tyr Asp Lys Gly Glu Arg Ser Leu Thr Asp Glu Glu 
    290                 295                 300                 


Lys Gln Leu Ile Ile Pro Leu Phe Lys Arg Lys Ser Lys Arg Thr Phe 
305                 310                 315                 320 


Pro Phe Glu Asp Ile Ala Lys Lys Ile Ala Gly Lys Asn Asn Tyr Cys 
                325                 330                 335     


Tyr Ile Lys Ser Asn Glu Glu Lys Ala Tyr Lys Phe Asn Tyr Gln Met 
            340                 345                 350         


Asp Thr Gln Val Ser Gly Ser Pro Val Asn Ala Gln Leu Glu Glu Ile 
        355                 360                 365             


Phe Gly Glu Asp Trp Leu Ser Ser Val Cys Glu Val Tyr Thr Leu Ala 
    370                 375                 380                 


Asn Asn Lys Ser Arg Phe Gln Ile Met Asn Asp Val Trp His Val Leu 
385                 390                 395                 400 


Phe Phe Tyr Asp Asp Glu Asp Lys Leu Lys Glu Phe Ala Lys Asp Arg 
                405                 410                 415     


Leu Gln Leu Asp Asp Glu Leu Ala Glu Lys Phe Ser Lys Ile Ser Ile 
            420                 425                 430         


Pro Asn Asp Tyr Ala Ala Leu Ser Leu Lys Ala Ile Arg Lys Ile Met 
        435                 440                 445             


Pro Tyr Leu Arg Asp Tyr Gly Leu Ile Tyr Ser Glu Ala Val Phe Leu 
    450                 455                 460                 


Ala Asn Leu Val Asp Val Leu Pro Gln His Val Trp Gly Ile Lys Glu 
465                 470                 475                 480 


Met Arg Glu Ala Ala Ile Glu Asn Val Ile Asp Val Met His Ser Tyr 
                485                 490                 495     


Asp Lys Asn Asn Ile Asp Gly Ile Thr Leu Glu Gly Cys Val Lys Thr 
            500                 505                 510         


Phe Leu Lys Glu Arg Tyr Gln Val Ser Asp Asp Asn Leu Lys Lys Leu 
        515                 520                 525             


Tyr His Pro Ser Met Leu Asp Pro Tyr Pro Arg Gln Arg Pro Asn Asp 
    530                 535                 540                 


Glu Gly Val Phe Gln Leu Gly Ser Pro Arg Ile Ser Ser Val Lys Asn 
545                 550                 555                 560 


Pro Met Ala Met His Ser Leu Phe Arg Leu Arg Lys Val Val Asn Leu 
                565                 570                 575     


Met Leu Lys Lys Gly Tyr Ile Thr Pro Asp Thr Lys Val His Ile Glu 
            580                 585                 590         


Phe Ser Arg Asp Leu Asn Asp Ala Asn Lys Arg Trp Ala Ile Gln Thr 
        595                 600                 605             


Trp Gln Arg Glu Asn Glu Lys Glu Arg Glu Arg Cys Arg Lys Glu Ile 
    610                 615                 620                 


Glu Lys Tyr Ile Ala Ser Pro Ser Asp Thr Asp Ile Leu Lys Tyr Gln 
625                 630                 635                 640 


Leu Trp Glu Glu Gln Asn His Lys Cys Leu Tyr Thr Gly Asp Glu Ile 
                645                 650                 655     


Arg Ile Thr Asp Phe Leu Gly Asp Asn Pro Lys Tyr Asp Ile Glu His 
            660                 665                 670         


Thr Ile Pro Arg Ser Ala Gly Gly Asp Ser Thr Lys Met Asn Leu Thr 
        675                 680                 685             


Leu Cys Ser Ser Arg Phe Asn Arg Asp Glu Lys Lys Thr Lys Leu Pro 
    690                 695                 700                 


Ala Glu Leu Val Asn His Asp Glu Ile Leu Glu Arg Ile Ser Asn Trp 
705                 710                 715                 720 


Lys Glu Lys Tyr Glu Asp Ile Asp Lys Gln Ile Arg Lys Ile Ser Thr 
                725                 730                 735     


Lys Gly Ala Thr Ser Lys Glu Asp Lys Asp Ser Lys Ile Arg Arg Arg 
            740                 745                 750         


His Tyr Leu Ile Leu Gln Arg Asp Tyr Trp Arg Gly Lys Tyr Gln Arg 
        755                 760                 765             


Phe Thr Met Thr Glu Val Pro Glu Gly Phe Ser Arg Arg Gln Gly Thr 
    770                 775                 780                 


Asp Ile Ser Val Ile Ser Arg Tyr Ala Arg Met Tyr Leu Lys Ser Val 
785                 790                 795                 800 


Phe Lys Gln Val Tyr Ile Val Lys Gly Ile Ala Thr Ser Asp Phe Arg 
                805                 810                 815     


Lys Met Trp Gly Ile Gln Asp Glu Tyr Ala Lys Lys Glu Arg Val Asn 
            820                 825                 830         


His Val His His Cys Ile Asp Ala Ile Thr Ile Ala Cys Ile Gly Lys 
        835                 840                 845             


Ala Glu Tyr Asp Lys Leu Ala Asp Tyr Arg His Gln Tyr Asp Asp Tyr 
    850                 855                 860                 


Leu Phe Gln Gly Lys Thr Lys Pro Thr Phe Asp Lys Pro Trp Pro Ser 
865                 870                 875                 880 


Phe Val Ile Asp Ile Lys His Ile Gln Asp Glu Leu Leu Ile Ala His 
                885                 890                 895     


Tyr Ala Lys Asp Asn Leu Glu Lys Phe Thr Lys Lys Asn Leu Arg Gly 
            900                 905                 910         


Lys Asp Gly Arg Ile Ile Arg Asp Lys Phe Gly Asn Lys Leu Gln Met 
        915                 920                 925             


Lys Gly Asp Thr Ser Arg Asn Ser Leu His Asn Asp Lys Phe Tyr Gly 
    930                 935                 940                 


Ala Ile Asn His Glu Gly Glu Ile Lys Tyr Val Tyr Arg Thr Asn Ile 
945                 950                 955                 960 


Glu Ala Leu Asp Ala Lys Ser Ile Gly Asn Ile Val Asp Pro Thr Val 
                965                 970                 975     


Arg Ser Ile Ile Glu Ser Ala Ile Ala Ile His Gly Ser Leu Lys Ala 
            980                 985                 990         


Ala Ile Asn Lys Gly Ile Trp Met  Asn Glu Glu Lys His  Ile Pro Ile 
        995                 1000                 1005             


Lys Lys  Val Arg Val Phe Arg  Asn Asp Val Lys Lys  Pro Ile Glu 
    1010                 1015                 1020             


Leu Lys  Asp Gln Arg Asp Leu  Ser Asp Lys Asp Tyr  Lys Gln His 
    1025                 1030                 1035             


Tyr Tyr  Val Thr Asn Asp Gly  Asn Tyr Met Met Leu  Ile Phe Ser 
    1040                 1045                 1050             


Lys Leu  Asn Asn Lys Gly Arg  Glu Ile Arg Gln His  Val Val Ile 
    1055                 1060                 1065             


Asn Ser  Leu Glu Ala Thr Glu  Val Ile Lys Ser His  Lys Arg Ser 
    1070                 1075                 1080             


Gln Val  Gly Lys Val Val Trp  Glu Ser Tyr Tyr Ser  Lys Glu Gly 
    1085                 1090                 1095             


Phe Asp  Leu Ile Tyr Lys Leu  Lys Ser Gly Thr Met  Ile Leu Leu 
    1100                 1105                 1110             


Tyr Glu  Asn Asn Arg Asp Glu  Val Trp Asp Leu Asp  Ile Arg Asn 
    1115                 1120                 1125             


Leu Gln  Lys Arg Leu Tyr Ile  Ile Thr Lys Met Lys  Tyr Asp Gly 
    1130                 1135                 1140             


Arg Ile  Tyr Met Met His His  Gln Glu Ala Arg Glu  Lys Lys Asp 
    1145                 1150                 1155             


Leu Glu  Glu Lys Ser Gly Ser  Phe Lys Ile Asn Glu  Asp Tyr Arg 
    1160                 1165                 1170             


Pro Leu  Ile Val Asn Ser Tyr  Asn Gln Phe Asn Ala  Leu Val Gln 
    1175                 1180                 1185             


Asp Leu  Asp Phe Glu Ile Asn  Asp Leu Gly Glu Ile  Arg Arg Leu 
    1190                 1195                 1200             


Ile 
    


<210>  767
<211>  1209
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  767

Met Lys Lys Asp Tyr Ile Leu Gly Leu Asp Ile Gly Ile Ser Ser Val 
1               5                   10                  15      


Gly Trp Gly Leu Leu Glu Leu Asp Asp Asn Asn Asn Pro Tyr Lys Ile 
            20                  25                  30          


Ile Asp Val Gly Ser Arg Ile Phe Thr Pro Gly Glu Val Glu Lys Thr 
        35                  40                  45              


Gly Asp Ser Arg Ala Lys Glu Arg Arg Glu Lys Arg Gly Ala Arg Arg 
    50                  55                  60                  


Ile Ala Arg Arg Arg Glu Phe Arg Leu Asp Arg Val Lys Asn Leu Leu 
65                  70                  75                  80  


Tyr Glu Asn Gly Tyr Leu Lys Gly Asn Ile Asn Ser Asn Ile Val Ser 
                85                  90                  95      


Glu Lys Asn Glu Glu Leu Ser Ile Ile Phe Asn Asn Met Ile Asn Asn 
            100                 105                 110         


Tyr Tyr Asn Gly Lys Asn Thr Asn Pro Tyr Ile Leu Lys Val Glu Ala 
        115                 120                 125             


Leu Asp Arg Lys Leu Thr Asp Glu Glu Leu Ser Ile Ile Leu Val His 
    130                 135                 140                 


Tyr Ala Lys Lys Arg Gly Tyr Lys Ser Asn Arg Glu Glu Ala Ser Asp 
145                 150                 155                 160 


Lys Glu Ser Gly Lys Val Leu Thr Ala Ile Lys Glu Asn Glu Lys Ile 
                165                 170                 175     


Met Lys Asp Asn Asn Tyr Arg Thr Ile Ser Glu Met Tyr Ile Lys Asp 
            180                 185                 190         


Asp Lys Phe Lys Asn Lys Ile Lys Asn Ser Pro Asn Asp Tyr Lys Ile 
        195                 200                 205             


Ser Val Thr Asn Glu Met Tyr Leu Glu Glu Ile Asn Lys Val Leu Asp 
    210                 215                 220                 


Ser Gln Ile Lys Phe Gly Leu Ile Thr Asp Asp Phe Lys Asn Gln Tyr 
225                 230                 235                 240 


Leu Glu Ile Tyr Asn Ser Arg Arg Ser Tyr Ala Lys Gly Pro Gly Gly 
                245                 250                 255     


Asn Ser Pro Tyr Gly Gly Asp Leu Ile Glu Arg Met Thr Gly Lys Cys 
            260                 265                 270         


Asn Phe Asp Gly Asn Pro Arg Ala Pro Lys Arg Ala Phe Ser Ser Glu 
        275                 280                 285             


Ile Tyr Val Ala Leu Thr Lys Leu Val Asn Leu Lys Tyr Lys Val Asn 
    290                 295                 300                 


Asp Gly Glu Tyr Ile Lys Leu Ser Glu Lys Glu Ile Lys Asn Ile Ile 
305                 310                 315                 320 


Glu Lys Ala Lys Glu Lys Lys Ser Ile Ser Tyr Lys Asp Leu Thr Lys 
                325                 330                 335     


Met Ile Gly Asn Asn Ile Asp Phe Lys Asp Leu Thr Leu Ser Lys Lys 
            340                 345                 350         


Glu Tyr Ile Lys Leu Ile Glu Glu Leu Lys Lys Lys Leu Asn Ile Lys 
        355                 360                 365             


Lys Asp Thr Lys Ile Asn Ile Asn Glu Leu Ser Ser Glu Asp Lys Glu 
    370                 375                 380                 


Leu Tyr Asn Lys Leu Cys Asn Asp Lys Leu Leu Ser Lys Thr Leu Ile 
385                 390                 395                 400 


Glu Leu Lys Gly Tyr His Ile Leu Lys Asp Ser Val Ile Lys Ala Tyr 
                405                 410                 415     


Ser Lys Asp Ile Trp Asp Glu Val Lys Asp Asn Met Tyr Leu Leu Asp 
            420                 425                 430         


Asp Leu Ala Leu Tyr Cys Thr Asn Tyr Lys Val Asn Glu Asp Ile Leu 
        435                 440                 445             


Asn Lys Ile Lys Glu Ser Glu Asn Ile Asp Asn Ile Phe Asn Asp Ile 
    450                 455                 460                 


His Phe Val Glu Leu Leu Pro Asn Phe Lys Asp His Leu Met Leu Ser 
465                 470                 475                 480 


Thr Glu Leu Ile Arg Lys Leu Ile Pro Ile Met Tyr Gly Gly Asn Thr 
                485                 490                 495     


Tyr Asp Lys Ala Met Asn Ile Leu Gly Tyr Asn His Ser Asp Ile Asn 
            500                 505                 510         


Gly Asp Lys Glu Lys Lys Asp Leu Leu Val Pro Ile Cys Val Asp Asp 
        515                 520                 525             


Asn Ile Thr Asn Gln Arg Val Ile Arg Ser Leu Thr Gln Thr Arg Lys 
    530                 535                 540                 


Val Ile Asn Ser Ile Ile Lys Lys Tyr Gly Met Pro Lys Ile Ile Asn 
545                 550                 555                 560 


Ile Glu Thr Ala Arg Glu Leu Ala Lys Ser Arg Gln Glu Arg Asn Glu 
                565                 570                 575     


Ile Ala Lys Asn Asn Val Glu Lys Gln Ala Glu Asn Glu Lys Ile Lys 
            580                 585                 590         


Asn Gln Leu Val Glu Leu Gly Leu Phe Val Ser Thr Asp Lys Ile Ser 
        595                 600                 605             


Ser Thr Asp Leu Leu Lys Tyr Lys Leu Trp Lys Glu Gln Asn Glu Phe 
    610                 615                 620                 


Cys Gly Tyr Ser Met Lys Lys Ile Ser Ile Glu Asp Leu Phe Gly Asn 
625                 630                 635                 640 


Asn Ile Val Gln Ile Asp His Ile Leu Pro Tyr Ser Arg Thr Tyr Asn 
                645                 650                 655     


Asp Asn Tyr Leu Asn Lys Thr Leu Val Tyr Thr Lys Glu Asn Gln Glu 
            660                 665                 670         


Lys Gly Asn Arg Thr Pro Tyr Glu Trp Phe Gly Lys Thr Asp Lys Trp 
        675                 680                 685             


Asp Glu Tyr Glu Thr Tyr Ile Asn Asn Leu Thr Ile Ser Gln Lys Lys 
    690                 695                 700                 


Lys Asp Asn Tyr Leu Leu Lys Lys Leu Asp Phe Asp Thr Glu Arg Glu 
705                 710                 715                 720 


Met Arg Ser Gln Asn Leu Asn Asp Thr Lys Tyr Ile Ser Lys Glu Leu 
                725                 730                 735     


Ser Ser Leu Ile Lys Ala Tyr Leu Asn Val Glu Lys Val Asn Met Tyr 
            740                 745                 750         


Pro Gly Ala Ile Thr Ala Lys Leu Arg Ala Arg Trp Gly Phe Asn Arg 
        755                 760                 765             


Leu Thr His Ser Tyr Leu Ser Lys Asn Tyr Ile Leu Pro Lys Asn Ile 
    770                 775                 780                 


Lys Glu Asp Ile Asn Lys Asp Arg Asp Asn His Leu His His Ala Met 
785                 790                 795                 800 


Asp Ala Leu Val Ile Ala Ser Ile Thr Pro Ser Leu Gln Gln Lys Ile 
                805                 810                 815     


Thr Leu Tyr Glu Lys Phe Ser Arg Tyr Ile Asp Gly Leu Thr Glu Leu 
            820                 825                 830         


Gln Ile Lys Asn Ile Ser Tyr Ile Lys Ile Glu Asp Ser Glu Gly Gln 
        835                 840                 845             


Tyr Tyr Asn Asp Asp Thr Asp Glu Val Glu Thr Ile Asp Ile Arg Glu 
    850                 855                 860                 


Tyr Ile Lys Gln Gln Leu Ala Glu Asn Asn Ile Tyr Tyr Asn Arg His 
865                 870                 875                 880 


Asn Ile Ser Lys Leu Glu Phe Pro Leu Pro Tyr Glu Asn Phe Asp Lys 
                885                 890                 895     


Glu Ala Lys Leu Arg Val Tyr Glu Gln Asp Leu Asp Thr Leu Lys Trp 
            900                 905                 910         


Asn Leu Lys Ser Tyr Asn Thr Tyr Thr Gln Lys Asp Ile Asp Asn Val 
        915                 920                 925             


His Thr Leu Ile Pro Ser Ile Ala Lys Thr Lys Val Ser Gly Lys Met 
    930                 935                 940                 


His Glu Glu Thr Tyr Tyr Gly Ile Lys Glu Ile Gly Lys Asp Glu Asp 
945                 950                 955                 960 


Lys Lys Ile Tyr Lys Thr Leu Arg Thr Pro Ile Glu Lys Val Lys Arg 
                965                 970                 975     


Lys Asp Leu Asp Asn Ile Pro Asp Arg Leu Gly Gly Ser Lys Asp Val 
            980                 985                 990         


Tyr Glu Ser Leu Val Glu Trp Phe  Gly Asp Tyr Glu Val  Gly Glu Glu 
        995                 1000                 1005             


Val Leu  Lys Ala Asn Gly Asn  Lys Tyr Pro Val Ser  Lys Ser Asp 
    1010                 1015                 1020             


Lys Glu  His Lys Glu Ile Lys  Lys Leu Lys Leu Tyr  Ser Glu Tyr 
    1025                 1030                 1035             


Lys Asn  Thr Gly His Met Ile  Asn Lys Ser Asn Val  Glu Lys Gly 
    1040                 1045                 1050             


Gly Ile  Tyr Arg Ile Asp Val  Phe Lys Ser Lys Asp  Lys Glu Asp 
    1055                 1060                 1065             


Asp Lys  Leu Tyr Phe Ala Ala  Tyr Asp Ile Leu Glu  Ile Lys Lys 
    1070                 1075                 1080             


Ile Asn  Thr Leu Lys Glu Lys  Asn Arg Val Asp Asp  Asn Phe Asn 
    1085                 1090                 1095             


Ile Lys  Leu Asp Tyr Gly Gln  Gly Lys Asn Ser Lys  Ile Met Ser 
    1100                 1105                 1110             


Tyr Thr  Asp Ile Ile Asn Asn  Tyr Asp Leu Tyr Ile  Ser Leu Asn 
    1115                 1120                 1125             


Lys Asn  Asp Leu Ile Lys Ile  Thr Thr Asn Asp Gly  Lys Glu Ser 
    1130                 1135                 1140             


Val Ala  Tyr Val Val Gly Cys  Ser Ser Gly Met Leu  Glu Val Lys 
    1145                 1150                 1155             


Ser Lys  Leu Gly Asp Gly Tyr  Asp Ile Ile Lys Thr  Asp Leu Asn 
    1160                 1165                 1170             


Glu Asn  Asn Ile Phe Ser Lys  Gln Arg Ala Gln Tyr  Gln Ile Thr 
    1175                 1180                 1185             


Val Ser  Thr Ile Lys Glu Ile  Lys Lys Leu Ser Ile  Ser Val Leu 
    1190                 1195                 1200             


Gly Glu  Ile Ser Gly Leu 
    1205                 


<210>  768
<211>  1210
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  768

Met Arg Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Ser Val Ile Lys Glu Gln Thr Glu Arg Ile Trp Ile 
            20                  25                  30          


Glu Met Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile Leu Gly 
        35                  40                  45              


Asp Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg Thr Arg 
    50                  55                  60                  


Phe Arg Gly Val Arg Arg Leu Arg Glu Arg Gln Leu Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Lys Val Leu Lys Ile Leu Gly Phe Leu Pro Glu His Tyr 
                85                  90                  95      


Leu Lys Gly Ile Asp Phe Glu Lys His Thr Gly Lys Phe Leu Ser Gly 
            100                 105                 110         


Ser Glu Pro Lys Leu Pro Trp Val Lys Asp Arg Trp Gly Lys Tyr Ser 
        115                 120                 125             


Phe Leu Phe Gln Thr Ala Phe Asn Glu Met Leu Ala Asp Phe Ala Lys 
    130                 135                 140                 


His Gln Pro Ser Leu Val Phe Asp Gly Lys Lys Ile Pro Tyr Asp Trp 
145                 150                 155                 160 


Thr Val Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Glu Met Ile Ser Lys 
                165                 170                 175     


Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr 
            180                 185                 190         


Tyr Gln Leu Arg Gly Glu Glu Glu Glu Asp Asn Ala Gly Lys Ser Val 
        195                 200                 205             


Glu Phe Tyr Ala Leu Lys Val Leu Ser Val Glu Ala Thr Asp Glu Lys 
    210                 215                 220                 


Lys Gly Arg Asp Ile Trp Tyr Asn Val His Leu Glu Asn Gly Trp Val 
225                 230                 235                 240 


Tyr Arg Arg Ser Ser Asn Val Pro Leu Asp Trp Glu Gly Arg Val Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Asp Leu Asn Pro Asp Gly Thr Pro Lys Leu 
            260                 265                 270         


Asp Lys Tyr Gly Glu Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp 
        275                 280                 285             


Asp Trp Met Leu Ile Lys Lys Arg Thr Glu Ala Asp Ile Thr Gly Thr 
    290                 295                 300                 


His Lys Thr Ile Gly Ser Tyr Ile Tyr Asp Ala Leu Leu Gln Glu Pro 
305                 310                 315                 320 


Gln Gln Lys Ile Ile Gly Lys Leu Val Lys Thr Val Glu Arg Lys Phe 
                325                 330                 335     


Tyr Lys Glu Glu Leu Asn Phe Ile Leu Gln Lys Gln Cys Ala Phe His 
            340                 345                 350         


Ala Glu Leu Gln Asp Arg Asp Leu Tyr Met Arg Cys Ile Glu Ala Leu 
        355                 360                 365             


Tyr Pro Gly Asn Glu Val Gln Arg Arg Asn Ile Ala Asn Arg Asp Phe 
    370                 375                 380                 


Ile Tyr Leu Leu Ile Asp Asp Val Leu Phe Tyr Gln Arg Pro Leu Lys 
385                 390                 395                 400 


Thr Lys Lys Ser Leu Ile Ala Asn Cys Pro Tyr Glu Glu Asn Glu Tyr 
                405                 410                 415     


Val Asn Arg Lys Thr Gly Glu Ile Lys Thr Ala Pro Leu Lys Cys Ile 
            420                 425                 430         


Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Tyr Phe Leu 
        435                 440                 445             


Ser Asn Ile Arg Val Tyr Gln Lys Glu Arg Glu Val Asn Gly Thr Leu 
    450                 455                 460                 


His Leu Asp Val Asp Val Thr Thr Glu Phe Leu Lys Thr Glu Asp Asp 
465                 470                 475                 480 


Tyr Val Ala Leu Phe Asp Trp Leu Asn Ser Arg Lys Glu Ile Asp Gln 
                485                 490                 495     


Lys Ala Phe Leu Arg Tyr Pro Ala Phe Gly Leu Lys Lys Glu Ile Asn 
            500                 505                 510         


Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys Phe Tyr Pro Cys Asn Glu 
        515                 520                 525             


Thr Arg His Ala Ile Leu Ser Arg Leu Glu Lys Ala Gly Ile Gly Ile 
    530                 535                 540                 


Asp Phe Leu Ser Asp Glu Lys Glu Thr Ala Leu Trp His Ile Leu Tyr 
545                 550                 555                 560 


Ser Val Ser Asp Lys Gln Glu Ile Glu Lys Ala Leu Ala Thr Phe Ala 
                565                 570                 575     


Val Lys Asn Gly Leu Asn Glu Ser Phe Val Glu Val Phe Lys Lys Phe 
            580                 585                 590         


Pro Pro Phe Lys Ser Glu Tyr Gly Ala Tyr Ser Ala Lys Ala Ile Lys 
        595                 600                 605             


Lys Leu Leu Pro Leu Met Arg Cys Gly Lys Tyr Trp Asp Met Ser Leu 
    610                 615                 620                 


Ile Asp Gly Thr Thr Lys Glu Arg Ile Glu Arg Ile Ile Ala Gly Glu 
625                 630                 635                 640 


Cys Asp Glu Ser Ile Gly Ala Lys Val Arg Glu Lys Ala Met Asn Leu 
                645                 650                 655     


Ser Asp Ile Ser Cys Phe Lys Arg Leu Pro Leu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Val Val Tyr Asn Arg His Ser Glu Asp Lys Glu Ile Thr Lys Trp Glu 
        675                 680                 685             


Thr Pro Ala Asp Ile Asp Ala Tyr Leu Ala Ala Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val 
705                 710                 715                 720 


Val Arg Asp Ile Trp Lys Gln Val Gly Lys Ile Asp Glu Ile Arg Ile 
                725                 730                 735     


Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Asp Lys Arg Lys Arg Met 
            740                 745                 750         


Thr Thr Gln Ile Leu Glu Asn Glu Asn Ala Asn Leu Arg Ile Lys Ala 
        755                 760                 765             


Leu Leu Ala Glu Phe Val Asn Pro Glu Tyr Gly Val Glu Asn Val Arg 
    770                 775                 780                 


Pro Tyr Ser Pro Ser Gln Gln Glu Ile Leu Arg Ile Tyr Glu Asp Ala 
785                 790                 795                 800 


Val Leu Lys Gly Glu Glu Gln Ile Pro Glu Asp Ile Asp Val Ile Leu 
                805                 810                 815     


Lys Lys Phe Asn Thr Ser Lys Leu Pro Thr Lys Ser Glu Phe Leu Arg 
            820                 825                 830         


Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Glu 
        835                 840                 845             


Leu Ile Pro Leu Ala Lys Leu Phe Thr Ala Ala Tyr Glu Ile Glu His 
    850                 855                 860                 


Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val 
865                 870                 875                 880 


Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp Asn Gln Leu Gly Tyr 
                885                 890                 895     


Glu Phe Ile Lys Asn His His Gly Gln Lys Val Glu Val Gly Phe Gly 
            900                 905                 910         


Lys Thr Val Glu Ile Phe Ser Val Asp Ser Tyr Glu Arg Phe Val Lys 
        915                 920                 925             


Glu Gln Tyr Ala Lys Ser Gly Ile Lys Met Lys Lys Leu Leu Met Asp 
    930                 935                 940                 


Asp Ile Pro Glu Gln Phe Ile Glu Arg Gln Leu Asn Asp Ser Arg Tyr 
945                 950                 955                 960 


Ile Ser Lys Val Val Lys Gly Leu Leu Ser Asn Ile Val Arg Glu Lys 
                965                 970                 975     


Asn Asp Ser Gly Glu Tyr Glu Pro Glu Ala Val Ser Lys Asn Ile Leu 
            980                 985                 990         


Val Cys Thr Gly Ser Val Thr Asp  Arg Leu Lys Lys Asp  Trp Gly Met 
        995                 1000                 1005             


Asn Asp  Val Trp Asn Ser Ile  Val Ser Pro Arg Phe  Glu Arg Leu 
    1010                 1015                 1020             


Asn Ala  Leu Thr Glu Thr Gln  Cys Phe Gly His Trp  Glu Asn Lys 
    1025                 1030                 1035             


Asp Gly  Lys Lys Val Phe Gln  Thr Ala Val Pro Leu  Glu Tyr Gln 
    1040                 1045                 1050             


Lys Gly  Phe Ser Lys Lys Arg  Ile Asp His Arg His  His Ala Met 
    1055                 1060                 1065             


Asp Ala  Ile Val Ile Ala Cys  Ala Thr Arg Asn His  Val Asn Tyr 
    1070                 1075                 1080             


Leu Asn  Asn Glu Ser Ala Ser  Arg Asn Ala Lys Ile  Ser Arg Tyr 
    1085                 1090                 1095             


Asp Leu  Gln Arg Leu Leu Cys  Asp Lys Ser Arg Gly  Asp Gly Asn 
    1100                 1105                 1110             


Gly Asn  Tyr Arg Trp Ile Ile  Lys Lys Pro Trp Thr  Thr Phe Thr 
    1115                 1120                 1125             


Gln Asp  Ala Arg Glu Ala Leu  Ser Gly Ile Ile Val  Ser Leu Lys 
    1130                 1135                 1140             


Gln Asn  Leu Arg Ile Ile Asn  Lys Thr Thr Asn Ile  Tyr Gln His 
    1145                 1150                 1155             


Phe Asp  Ala Asn Gly Thr Arg  Val Tyr Glu Lys Gln  Lys Ile Gly 
    1160                 1165                 1170             


Asp Ser  Trp Ala Ile Arg Lys  Pro Met His Lys Asp  Thr Val Phe 
    1175                 1180                 1185             


Gly Ala  Val Asn Leu Arg Lys  Ala Leu Ile Pro Gln  His Tyr Asn 
    1190                 1195                 1200             


Leu Thr  Leu Phe Ile Ser Ser  
    1205                 1210 


<210>  769
<211>  1213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  769

Met Glu Lys Arg Leu Gly Ile Asp Val Gly Thr Asn Ser Leu Gly Trp 
1               5                   10                  15      


Ala Ile Val Glu Val Asp Asp Glu Gly Cys Thr Leu Leu Asp Arg Gly 
            20                  25                  30          


Val Asp Ile Phe Gln Glu Gly Val Ala Arg Glu Lys Asn Gln Glu Lys 
        35                  40                  45              


Pro Ala Val Gln Asp Arg Thr Asp Ala Arg Ala Leu Arg Arg His Tyr 
    50                  55                  60                  


Phe Arg Arg Arg Leu Arg Lys Ile Ala Leu Leu Arg Val Leu Val Arg 
65                  70                  75                  80  


His Asp Leu Cys Pro Ala Leu Ser Glu Glu Gln Leu Asn Ala Trp Gln 
                85                  90                  95      


Gly Lys Gly Ile Tyr Pro Leu Asp Asp Ala Phe Ile Arg Trp Gln Arg 
            100                 105                 110         


Thr Asp Asp Asn Ala Asp Arg Asn Pro Tyr Arg Asp Arg Phe Arg Cys 
        115                 120                 125             


Leu Thr Glu Glu Leu Asp Leu Ala Cys Arg Glu Glu Arg Tyr Val Leu 
    130                 135                 140                 


Gly Arg Ala Leu Tyr His Leu Cys Gln Arg Arg Gly Phe Leu Ser Asn 
145                 150                 155                 160 


Arg Lys Asp Ala Gly Asn Glu Ala Glu Glu Gly Lys Val Lys Gln Glu 
                165                 170                 175     


Ile Lys Thr Leu Ser Ala Asp Met Ala Ala Ala Gly Cys Glu Tyr Leu 
            180                 185                 190         


Gly Glu Tyr Phe Tyr Arg Leu Tyr Gln Arg Lys Glu Lys Ile Arg Lys 
        195                 200                 205             


His Tyr Thr Ala Arg Asn Glu His Tyr Leu Ala Glu Phe Arg Ala Ile 
    210                 215                 220                 


Cys Arg Lys Gln Arg Leu Asp Glu Glu Leu Arg Gln Ala Leu Glu Lys 
225                 230                 235                 240 


Ala Ile Phe Tyr Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Val Gly 
                245                 250                 255     


Lys Cys Thr Phe Glu Lys Asp Lys Ser Arg Cys Pro Val Ser His Pro 
            260                 265                 270         


Arg Phe Glu Glu Phe Arg Met Leu Gln Phe Ile Asn Asn Val Arg Val 
        275                 280                 285             


Thr Gly Pro Gly Asp Ala Glu Pro Arg Pro Leu Ser Pro Glu Glu Val 
    290                 295                 300                 


Glu Ile Val Arg Pro Leu Phe Leu Arg Lys Ser Lys Pro His Phe Asp 
305                 310                 315                 320 


Phe Glu Asp Ile Ala Lys Lys Ile Ala Gly Lys Gly Asn Tyr Ala Cys 
                325                 330                 335     


Arg Gly Glu Leu Leu Gln Ala Pro Tyr Arg Phe Asn Cys Ala Gly Lys 
            340                 345                 350         


Ser Thr Val Thr Gly Ser Pro Val Ser Ala Leu Phe Ala Ser Val Phe 
        355                 360                 365             


Gly Asp Asp Trp Gln Arg Glu Ile Cys Ser Leu Tyr Thr Leu Gly Ala 
    370                 375                 380                 


Gly Lys Asn Glu Glu Gln Ile Leu Asn Asp Val Trp His Val Leu Phe 
385                 390                 395                 400 


Ala Phe Glu Asp Glu Glu Arg Leu Gln Ala Trp Leu Gln Glu Lys Leu 
                405                 410                 415     


Gln Leu Ser Ala Asp Asp Ala Lys Thr Leu Ala Ala Phe Arg Leu Pro 
            420                 425                 430         


Gln Gly Tyr Ala Ser Leu Ser Leu Asn Val Ile Asn Lys Ile Leu Pro 
        435                 440                 445             


Tyr Leu Arg Arg Gly Tyr Arg Tyr Asp Glu Ala Val Phe Phe Ala Asn 
    450                 455                 460                 


Leu Gly Lys Val Leu Pro Ala Glu Val Tyr Ala Asp Pro Ala Arg Arg 
465                 470                 475                 480 


Arg Glu Val Glu Gln Gly Ile Ser Ala Gln Met Asp Asp Leu Gly Asp 
                485                 490                 495     


Gly Glu Lys Thr Arg Lys Glu Glu Arg Ile Arg Asp Tyr Leu Arg Arg 
            500                 505                 510         


Tyr Trp Gly Val Glu Glu Lys Arg Leu Glu Arg Leu Tyr His Pro Ser 
        515                 520                 525             


Met Ile Glu Ala Tyr Pro Ser Ala Ser Pro Asn Gly Gln Gly Leu Leu 
    530                 535                 540                 


Gln Leu Gly Ser Pro Arg Ile Pro Ala Val Arg Asn Pro Met Ala Met 
545                 550                 555                 560 


Arg Ala Leu Phe Arg Leu Arg Val Leu Leu Asn Glu Leu Leu Arg Glu 
                565                 570                 575     


Gly Lys Ile Asp Arg Gly Thr Lys Ile Asn Ile Glu Leu Ala Arg Glu 
            580                 585                 590         


Leu Asn Asp Ala Asn Lys Arg Lys Ala Ile Glu Arg Tyr Gln Arg Glu 
        595                 600                 605             


Arg Glu Ala Glu Asn Arg Lys Tyr Ala Glu Glu Ile Arg Gln Gln Tyr 
    610                 615                 620                 


Val Glu Ala Thr Gly His Ser Ile Glu Pro Ser Glu Glu Asp Ile Leu 
625                 630                 635                 640 


Lys Tyr Arg Leu Trp Val Glu Gln Gly His Cys Ser Leu Tyr Thr Gly 
                645                 650                 655     


Lys Thr Ile Gly Ile Ser Glu Phe Leu Gly Asp Gly Arg Arg Tyr Asp 
            660                 665                 670         


Ile Glu His Thr Val Pro Arg Ser Arg Gly Gly Glu Asp Ala Gln Tyr 
        675                 680                 685             


Asn Lys Thr Leu Cys Glu Glu Glu Phe Asn Arg Asp Ile Lys Arg Asp 
    690                 695                 700                 


Lys Leu Pro Ser Glu Leu Ala Asn His Thr Ala Ile Leu Ala Cys Val 
705                 710                 715                 720 


Glu Ser Cys Gly Trp Phe Glu Arg Ile Glu Gly Leu Glu His Arg Val 
                725                 730                 735     


Trp Gly Ala Asp Arg Arg Ala Lys Ser Ala Thr Thr Lys Ser Ala Lys 
            740                 745                 750         


Asp Phe Ala Ile Gln Asp Arg His Tyr Trp Arg Met His Leu Asp Tyr 
        755                 760                 765             


Trp Arg Arg Lys Cys Glu Ala Phe Thr Met Thr Glu Val Pro Glu Gly 
    770                 775                 780                 


Phe Ser Asn Arg Gln Gly Val Asp Val Gly Ile Ile Gly Arg Tyr Ala 
785                 790                 795                 800 


Arg Leu Tyr Leu Gln Thr Val Phe Asp Arg Val Tyr Thr Val Lys Gly 
                805                 810                 815     


Ala Thr Thr Ala Ala Phe Arg Arg Met Trp Gly Leu Gln Glu Glu Tyr 
            820                 825                 830         


Ala Ala Lys Glu Arg Val Asn His Val His His Cys Ile Asp Ala Ile 
        835                 840                 845             


Thr Ile Ala Cys Ile Gly Ser Arg Glu Tyr Ala Leu Trp Ala Gln Tyr 
    850                 855                 860                 


Val Arg Gln Asp Glu Leu Tyr Arg Trp Gly Glu Ala Asp Lys Pro Arg 
865                 870                 875                 880 


Cys Gly Lys Pro Trp Pro Thr Phe Thr Glu Asp Val Arg Ala Thr Ala 
                885                 890                 895     


Asp Glu Leu Leu Ile Ser His His Thr Pro Asp Asn Met Pro Lys Gln 
            900                 905                 910         


Ser Arg Lys Arg Leu Arg Val Arg Gly Lys Val Gln Arg Asn Asp Arg 
        915                 920                 925             


Gly Glu Val Lys Tyr Met Gln Gly Asp Thr Ala Arg Gly Ala Leu His 
    930                 935                 940                 


Gln Gln Thr Phe Tyr Gly Ala Ile Glu Arg Asp Gly Glu Ile Arg Tyr 
945                 950                 955                 960 


Val Val Arg Lys Ser Leu Asp Gln Leu Leu Pro Ala Asp Val Glu Lys 
                965                 970                 975     


Ile Val Asp Glu Ala Val Arg Gln Lys Val Arg Glu Ala Val Glu Ala 
            980                 985                 990         


Tyr Gly Phe Lys Glu Ala Thr Asp  Pro Thr Lys His Thr  Val Trp Met 
        995                 1000                 1005             


Asn Glu  Glu Lys Gln Ile Pro  Ile Arg Lys Val Arg  Ile Leu Thr 
    1010                 1015                 1020             


Gly Val  Lys Asn Pro Ile Leu  Leu Lys Pro His Arg  Asp Val Ser 
    1025                 1030                 1035             


Val Lys  Glu Tyr Lys Arg Tyr  Tyr His Val Val Asn  Asp Gly Asn 
    1040                 1045                 1050             


Tyr Cys  Met Ala Ile Tyr Glu  Gly Arg Asp Arg Gln  Gly Arg Leu 
    1055                 1060                 1065             


Lys Arg  Thr Phe Glu Ile Val  Asn Lys Leu Glu Ala  Ala Arg Tyr 
    1070                 1075                 1080             


Phe Lys  Arg Ser Ala Asp Arg  Glu Ser Arg Pro Asp  Leu Val Pro 
    1085                 1090                 1095             


Leu Ser  Asp Val Asn Gly Tyr  Ser Leu Lys Tyr Leu  Leu Lys Thr 
    1100                 1105                 1110             


Gly Thr  Met Val Leu Phe Tyr  Glu Asn Ser Pro Asp  Glu Leu Tyr 
    1115                 1120                 1125             


Glu Cys  Ser Val Gly Glu Leu  Ala Lys Arg Leu Tyr  Lys Val Val 
    1130                 1135                 1140             


Gly Met  Ala Gln Asp Gly Arg  Val Gln Phe Leu Phe  His Gln Glu 
    1145                 1150                 1155             


Ala Arg  Asp Gln Lys Ala Val  Thr Ala Glu Cys Gly  Met Gly Ile 
    1160                 1165                 1170             


Ser Val  Phe Asp Ala Arg His  Pro Ala Pro Lys Leu  Arg Ile Arg 
    1175                 1180                 1185             


Val Ser  Asn Phe Lys Met Phe  Val Glu Gly Tyr Asp  Phe Glu Leu 
    1190                 1195                 1200             


Thr Val  Thr Gly Glu Val Lys  Phe Lys Arg 
    1205                 1210             


<210>  770
<211>  1213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  770

Met Glu Lys Arg Leu Gly Ile Asp Val Gly Thr Asn Ser Leu Gly Trp 
1               5                   10                  15      


Ala Ile Val Glu Val Asp Asp Glu Gly Cys Thr Leu Leu Asp Arg Gly 
            20                  25                  30          


Val Asp Ile Phe Gln Glu Gly Val Ala Arg Glu Lys Asn Gln Glu Lys 
        35                  40                  45              


Pro Ala Val Gln Asp Arg Thr Asp Ala Arg Ala Leu Arg Arg His Tyr 
    50                  55                  60                  


Phe Arg Arg Arg Leu Arg Lys Ile Ala Leu Leu Arg Val Leu Val Arg 
65                  70                  75                  80  


His Asp Leu Cys Pro Ala Leu Ser Glu Glu Gln Leu Asn Ala Trp Gln 
                85                  90                  95      


Gly Lys Gly Ile Tyr Pro Leu Asp Asp Ala Phe Ile Arg Trp Gln Arg 
            100                 105                 110         


Thr Asp Asp Asn Ala Asp Arg Asn Pro Tyr Arg Asp Arg Phe Arg Cys 
        115                 120                 125             


Leu Thr Glu Glu Leu Asp Leu Ala Cys Arg Glu Glu Arg Tyr Val Leu 
    130                 135                 140                 


Gly Arg Ala Leu Tyr His Leu Cys Gln Arg Arg Gly Phe Leu Ser Asn 
145                 150                 155                 160 


Arg Lys Asp Ala Gly Asn Glu Ala Glu Glu Gly Lys Val Lys Gln Glu 
                165                 170                 175     


Ile Lys Thr Leu Ser Ala Asp Met Ala Ala Ala Gly Cys Glu Tyr Leu 
            180                 185                 190         


Gly Glu Tyr Phe Tyr Arg Leu Tyr Gln Arg Lys Glu Lys Ile Arg Lys 
        195                 200                 205             


His Tyr Thr Ala Arg Asn Glu His Tyr Leu Ala Glu Phe Arg Ala Ile 
    210                 215                 220                 


Cys Arg Lys Gln Arg Leu Asp Glu Glu Leu Arg Gln Ala Leu Glu Lys 
225                 230                 235                 240 


Ala Ile Phe Tyr Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Val Gly 
                245                 250                 255     


Lys Cys Thr Phe Glu Lys Asp Lys Ser Arg Cys Pro Val Ser His Pro 
            260                 265                 270         


Arg Phe Glu Glu Phe Arg Met Leu Gln Phe Ile Asn Asn Val Arg Val 
        275                 280                 285             


Thr Gly Pro Gly Asp Ala Glu Pro Arg Pro Leu Ser Pro Glu Glu Val 
    290                 295                 300                 


Glu Ile Val Arg Pro Leu Phe Leu Arg Lys Ser Lys Leu His Phe Asp 
305                 310                 315                 320 


Phe Glu Asp Ile Ala Lys Lys Ile Ala Gly Lys Gly Asn Tyr Ala Cys 
                325                 330                 335     


Arg Gly Glu Leu Leu Gln Ala Pro Tyr Arg Phe Asn Cys Ala Gly Lys 
            340                 345                 350         


Ser Thr Val Thr Gly Ser Pro Val Ser Ala Leu Phe Ala Ser Val Phe 
        355                 360                 365             


Gly Asp Asp Trp Gln Arg Glu Ile Cys Ser Leu Tyr Thr Leu Gly Ala 
    370                 375                 380                 


Gly Lys Asn Glu Glu Gln Ile Leu Asn Asp Val Trp His Val Leu Phe 
385                 390                 395                 400 


Ala Phe Glu Asp Glu Glu Arg Leu Gln Thr Trp Leu Gln Glu Lys Leu 
                405                 410                 415     


Gln Leu Ser Ala Asp Asp Ala Lys Thr Leu Ala Ala Phe Arg Leu Pro 
            420                 425                 430         


Gln Gly Tyr Ala Ser Leu Ser Leu Asn Val Ile Asn Lys Ile Leu Pro 
        435                 440                 445             


Tyr Leu Arg Arg Gly Tyr Arg Tyr Asp Glu Ala Val Phe Phe Ala Asn 
    450                 455                 460                 


Leu Gly Lys Val Leu Pro Ala Glu Val Tyr Ala Asp Pro Ala Arg Arg 
465                 470                 475                 480 


Arg Glu Val Glu Gln Gly Ile Ser Ala Gln Met Asp Asp Leu Gly Asp 
                485                 490                 495     


Gly Glu Gln Thr Arg Lys Glu Glu Arg Ile Arg Asp Tyr Leu Arg Arg 
            500                 505                 510         


Tyr Trp Gly Val Glu Glu Lys Arg Leu Glu Arg Leu Tyr His Pro Ser 
        515                 520                 525             


Met Ile Glu Ala Tyr Pro Ser Ala Ser Pro Asn Gly Gln Gly Leu Leu 
    530                 535                 540                 


Gln Leu Gly Ser Pro Arg Ile Pro Ala Val Arg Asn Pro Met Ala Met 
545                 550                 555                 560 


Arg Ala Leu Phe Arg Leu Arg Val Leu Leu Asn Glu Leu Leu Arg Glu 
                565                 570                 575     


Gly Lys Ile Asp Arg Gly Thr Lys Ile Asn Ile Glu Leu Ala Arg Glu 
            580                 585                 590         


Leu Asn Asp Ala Asn Lys Arg Lys Ala Ile Glu Arg Tyr Gln Arg Glu 
        595                 600                 605             


Arg Glu Ala Glu Asn Arg Lys Tyr Ala Glu Glu Ile Arg Gln Gln Tyr 
    610                 615                 620                 


Val Glu Ala Thr Gly His Ser Ile Glu Pro Ser Glu Glu Asp Ile Leu 
625                 630                 635                 640 


Lys Tyr Arg Leu Trp Val Glu Gln Gly His Cys Ser Leu Tyr Thr Gly 
                645                 650                 655     


Lys Thr Ile Gly Ile Ser Glu Phe Leu Gly Asp Gly Arg Arg Tyr Asp 
            660                 665                 670         


Ile Glu His Thr Val Pro Arg Ser Arg Gly Gly Glu Asp Ala Gln Tyr 
        675                 680                 685             


Asn Lys Thr Leu Cys Glu Glu Glu Phe Asn Arg Asp Ile Lys Arg Asp 
    690                 695                 700                 


Lys Leu Pro Ser Glu Leu Ala Asn His Thr Ala Ile Leu Ala Cys Val 
705                 710                 715                 720 


Glu Ser Cys Gly Trp Phe Glu Arg Ile Glu Gly Leu Glu His Arg Val 
                725                 730                 735     


Trp Gly Ala Asp Arg Arg Ala Lys Ser Ala Thr Thr Lys Ser Ala Lys 
            740                 745                 750         


Asp Phe Ala Ile Gln Asp Arg His Tyr Trp Arg Met His Leu Asp Tyr 
        755                 760                 765             


Trp Arg Arg Lys Cys Glu Ala Phe Thr Met Thr Glu Val Pro Glu Gly 
    770                 775                 780                 


Phe Ser Asn Arg Gln Gly Val Asp Val Gly Ile Ile Gly Arg Tyr Ala 
785                 790                 795                 800 


Arg Leu Tyr Leu Gln Thr Val Phe Asp Arg Val Tyr Thr Val Lys Gly 
                805                 810                 815     


Ala Thr Thr Ala Ala Phe Arg Arg Met Trp Gly Leu Gln Glu Glu Tyr 
            820                 825                 830         


Ala Ala Lys Glu Arg Val Asn His Val His His Cys Ile Asp Ala Ile 
        835                 840                 845             


Thr Ile Ala Cys Ile Gly Ser Arg Glu Tyr Ala Leu Trp Ala Gln Tyr 
    850                 855                 860                 


Val Arg Gln Asp Glu Leu Tyr Arg Arg Gly Glu Ala Asp Lys Pro Arg 
865                 870                 875                 880 


Cys Gly Lys Pro Trp Pro Thr Phe Thr Glu Asp Val Arg Ala Thr Ala 
                885                 890                 895     


Asp Glu Leu Leu Ile Ser His His Thr Pro Asp Asn Met Pro Lys Gln 
            900                 905                 910         


Ser Arg Lys Arg Leu Arg Val Arg Gly Lys Val Gln Arg Asn Asp Arg 
        915                 920                 925             


Gly Glu Val Lys Tyr Met Gln Gly Asp Thr Ala Arg Gly Ala Leu His 
    930                 935                 940                 


Gln Gln Thr Phe Tyr Gly Ala Ile Glu Arg Asp Gly Glu Ile Arg Tyr 
945                 950                 955                 960 


Val Val Arg Lys Ser Leu Asp Gln Leu Leu Pro Ala Asp Val Glu Lys 
                965                 970                 975     


Ile Val Asp Glu Ala Val Arg Gln Lys Val Arg Glu Ala Val Glu Ala 
            980                 985                 990         


Tyr Gly Phe Lys Glu Ala Thr Asp  Pro Thr Lys His Thr  Val Trp Met 
        995                 1000                 1005             


Asn Glu  Glu Lys Gln Ile Pro  Ile Arg Lys Val Arg  Ile Leu Thr 
    1010                 1015                 1020             


Gly Val  Lys Asn Pro Ile Leu  Leu Lys Pro His Arg  Asp Val Ser 
    1025                 1030                 1035             


Val Lys  Glu Tyr Lys Arg Tyr  Tyr His Val Val Asn  Asp Gly Asn 
    1040                 1045                 1050             


Tyr Cys  Met Ala Ile Tyr Glu  Gly Arg Asp Arg Gln  Gly Arg Leu 
    1055                 1060                 1065             


Lys Arg  Thr Phe Glu Ile Val  Asn Lys Leu Glu Ala  Ala Arg Tyr 
    1070                 1075                 1080             


Phe Lys  Arg Ser Ala Asp Arg  Glu Ser Arg Pro Asp  Leu Val Pro 
    1085                 1090                 1095             


Leu Ser  Asp Val Asn Gly Tyr  Ser Leu Lys Tyr Leu  Leu Lys Thr 
    1100                 1105                 1110             


Gly Thr  Met Val Leu Phe Tyr  Glu Asn Ser Pro Asp  Glu Leu Tyr 
    1115                 1120                 1125             


Glu Cys  Ser Val Gly Glu Leu  Ala Lys Arg Leu Tyr  Lys Val Val 
    1130                 1135                 1140             


Gly Met  Ala Gln Asp Gly Arg  Val Gln Phe Leu Phe  His Gln Glu 
    1145                 1150                 1155             


Ala Arg  Asp Gln Lys Ala Val  Thr Ala Glu Cys Gly  Met Gly Ile 
    1160                 1165                 1170             


Ser Val  Phe Asp Ala Arg His  Pro Ala Pro Lys Leu  Arg Ile Arg 
    1175                 1180                 1185             


Val Ser  Asn Phe Lys Met Phe  Val Glu Gly Tyr Asp  Phe Glu Leu 
    1190                 1195                 1200             


Thr Val  Thr Gly Glu Val Lys  Phe Lys Arg 
    1205                 1210             


<210>  771
<211>  1216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  771

Met Thr Lys Ile Leu Gly Ile Asp Thr Gly Thr Asn Ser Leu Gly Trp 
1               5                   10                  15      


Ala Ile Val Asp Lys Thr Ala Asp Asn Tyr Asn Leu Leu Asp Lys Gly 
            20                  25                  30          


Val Asn Ile Phe Gln Glu Gly Val Lys Ile Glu Lys Gly Ile Glu Ser 
        35                  40                  45              


Ser Lys Ala Ala Glu Arg Thr Glu His Arg Ala Thr Arg Val Arg Tyr 
    50                  55                  60                  


Tyr Arg Ile Lys Leu Arg Lys Ile Arg Leu Leu Arg Ile Leu Ser Asp 
65                  70                  75                  80  


Asn His Leu Cys Pro Pro Leu Ser Gln Glu Glu Leu Ser Thr Trp Arg 
                85                  90                  95      


Leu Lys Lys Val Tyr Pro Lys Asn Glu Leu Phe Met Gln Trp Gln Arg 
            100                 105                 110         


Thr Asp Asp Asp Thr Glu Lys Thr Pro Tyr Ala Tyr Arg His Lys Cys 
        115                 120                 125             


Ile His Glu Lys Leu Asp Leu Ser Asp Val Thr Asp Arg Tyr Ile Leu 
    130                 135                 140                 


Gly Arg Ala Phe Tyr His Ile Ile Gln Arg Arg Gly Phe Leu Ser Asn 
145                 150                 155                 160 


Arg Lys Glu Gln Gly Gly Glu Asp Ser Gly Lys Val Lys Glu Gly Ile 
                165                 170                 175     


Ser Thr Leu Thr His Glu Met Asn Glu Ala Gly Tyr Glu Tyr Leu Gly 
            180                 185                 190         


Asp Tyr Phe Tyr Ala Leu Tyr Asn Lys Gly Glu Lys Ile Arg Lys His 
        195                 200                 205             


Tyr Thr Ala Arg Asn Glu His Phe Leu Ser Glu Phe Lys Ala Ile Cys 
    210                 215                 220                 


Glu Lys Gln Asp Leu Glu Lys Glu Leu Gly Ser Glu Val Val Arg Asn 
225                 230                 235                 240 


Ile Glu Lys Ala Ile Phe Asp Gln Arg Pro Leu Lys Ser Gln Lys Gly 
                245                 250                 255     


Gln Val Gly Lys Cys Val Phe Glu Lys Asn Lys Thr Lys Cys Pro Ser 
            260                 265                 270         


Ser His Pro Met Tyr Glu Glu Phe Arg Met Leu Thr Phe Ile Asn Asn 
        275                 280                 285             


Ile Lys Ile Lys Thr Pro Asn Asp Asp Val Leu Arg Pro Leu Asn Thr 
    290                 295                 300                 


Gly Glu Arg Glu Lys Ile Met Pro Leu Phe Phe Arg Lys Ser Lys Lys 
305                 310                 315                 320 


Gln Phe Asp Phe Glu Asp Ile Thr Lys Lys Leu Ala Pro Lys Lys Met 
                325                 330                 335     


Tyr Gly Phe Tyr Lys Lys Glu Glu Asp Ala Glu Met Pro Phe Leu Phe 
            340                 345                 350         


Asn Tyr Pro Met Asp Thr Ser Val Ser Gly Cys Pro Val Thr Ala Ala 
        355                 360                 365             


Leu Lys Asp Val Phe Gly Asp Asp Trp Ile Thr Ala Ile Cys Glu Thr 
    370                 375                 380                 


Tyr Thr Leu Gly Glu Gly Lys Ser Arg Phe Asp Ile Val Asn Asp Ile 
385                 390                 395                 400 


Trp His Ala Leu Tyr Phe Tyr Ser Asp Lys Asp Lys Leu Ala Glu Phe 
                405                 410                 415     


Ala Ile Asn Arg Leu Gln Leu Ser Glu Asp Glu Ala Lys Ile Phe Ser 
            420                 425                 430         


Glu Ile Ser Leu Pro Ala Asp Tyr Ala Ser Leu Ser Leu Lys Ala Ile 
        435                 440                 445             


Cys Lys Ile Leu Pro Tyr Leu Arg Arg Gly Leu Ile Tyr Ser His Ala 
    450                 455                 460                 


Val Phe Leu Gly Asn Leu Cys Glu Val Met Pro Lys Tyr Glu Trp Ser 
465                 470                 475                 480 


Ile Lys Glu Met Arg Glu Thr Ile Ile Asp Asn Ile Ile Met Glu Met 
                485                 490                 495     


Asp Lys Cys Asp Thr Lys Asp Lys Arg Thr Phe Glu Val Cys Ile Lys 
            500                 505                 510         


Asp Tyr Leu Lys Gly Gln Tyr Asn Val Ser Asp Asn Ser Leu Lys Lys 
        515                 520                 525             


Leu Tyr His Pro Ser Met Ile Glu Thr Tyr Pro Arg Val Gln Tyr Thr 
    530                 535                 540                 


Asn Glu His Gly Val Tyr Gln Leu Gly Ser Pro Arg Ile Asp Ser Val 
545                 550                 555                 560 


Arg Asn Pro Met Ala Met Arg Ser Met Phe Arg Leu Arg Lys Leu Val 
                565                 570                 575     


Asn Arg Leu Leu Val Glu Gly Lys Ile Asp Gln Asn Thr Glu Val His 
            580                 585                 590         


Ile Glu Phe Ala Arg Glu Leu Asn Asp Ala Asn Lys Arg Asn Ala Ile 
        595                 600                 605             


Ala Leu Phe Val Lys Glu Asn Gln Ser Ala Asn Asp Glu Ala Arg Lys 
    610                 615                 620                 


Gln Ile Ile Thr Leu Phe Lys Lys Glu Thr Gly Thr Asp Ile Glu Pro 
625                 630                 635                 640 


Thr Asp Lys Asp Ile Leu Lys Tyr Val Leu Trp Glu Glu Gln Asn His 
                645                 650                 655     


Ile Cys Leu Tyr Thr Gly Lys Gln Ile Arg Ile Ser Asp Phe Ile Gly 
            660                 665                 670         


Ala Asn Pro Lys Phe Asp Ile Glu His Thr Ile Pro Arg Ser Val Gly 
        675                 680                 685             


Gly Asp Ser Thr Lys Met Asn Leu Thr Leu Cys Asp Ser Arg Phe Asn 
    690                 695                 700                 


Arg Glu Val Lys Lys Thr Lys Leu Pro Val Glu Leu Pro Asn His Asp 
705                 710                 715                 720 


Glu Ile Met Glu Arg Ile Lys Glu Trp Lys Lys Asn Tyr Glu Leu Leu 
                725                 730                 735     


Asp Ile Gln Ile Arg Lys Asn Lys Lys Leu Ser Lys Gly Ala Thr Thr 
            740                 745                 750         


Lys Glu Gln Lys Asp Thr Ile Ile Arg Lys Arg His Leu Leu Glu Leu 
        755                 760                 765             


Arg Arg Asp Tyr Trp Arg Gly Lys Tyr Glu Arg Phe Thr Met Glu Ser 
    770                 775                 780                 


Val Pro Glu Gly Phe Ser Arg Arg Gln Gly Thr Asp Ile Ser Val Ile 
785                 790                 795                 800 


Ser Lys Tyr Ala Arg Leu Tyr Leu Lys Ser Leu Phe Asn Arg Val Tyr 
                805                 810                 815     


Thr Val Lys Gly Ile Ala Thr Ser Asp Phe Arg Lys Ile Trp Gly Ile 
            820                 825                 830         


Gln Asp Ile Tyr Ser Lys Lys Glu Arg Ile Asn His Val His His Cys 
        835                 840                 845             


Ile Asp Ala Ile Val Ile Ala Cys Ile Gly Leu Asp Glu Tyr Asn Lys 
    850                 855                 860                 


Leu Gly Ala Tyr Tyr His Asp Glu Glu Asn Asn Lys Trp Tyr Gly Met 
865                 870                 875                 880 


Ser Lys Ala Ser Phe Lys Lys Pro Trp Ala Thr Phe Val Glu Asp Ile 
                885                 890                 895     


Lys Lys Ile Gln Asp Glu Ile Leu Val Tyr His Tyr Thr Pro Asp Asn 
            900                 905                 910         


Met Pro Lys Gln Gly Arg Arg Arg Ile Lys Ile Asp Gly Lys Lys Ile 
        915                 920                 925             


Leu Cys Lys Gly Asp Ala Ala Arg Gly Ser Leu His Asn Asp Thr Tyr 
    930                 935                 940                 


Tyr Gly Ala Ile Glu Asn Asp Gly Ala Val Lys Tyr Val Lys Arg Ile 
945                 950                 955                 960 


Asp Leu Ala Ser Leu Glu Glu Lys Asp Val Lys Asn Ile Val Asp Asp 
                965                 970                 975     


Thr Val Arg Ser Ile Ile Glu Ala Ala Ile Lys Glu Lys Gly Phe Lys 
            980                 985                 990         


Asp Ala Met Ala Ser Thr Ile Trp  Met Asn Glu Glu Lys  Arg Val Pro 
        995                 1000                 1005             


Ile Lys  Lys Val Arg Cys Phe  Thr Pro Ser Ile Thr  Lys Pro Leu 
    1010                 1015                 1020             


Asn Ile  Arg Lys Gln Arg Asp  Val Ser Ile Lys Glu  Tyr Lys Gln 
    1025                 1030                 1035             


Gln Tyr  His Val Ala Asn Asp  Ser Asn Tyr Leu Leu  Ala Leu Tyr 
    1040                 1045                 1050             


Ile Gly  Thr Asp Asn Lys Gly  Lys Glu Lys Arg Glu  Phe Glu Ile 
    1055                 1060                 1065             


Val Asn  Ile Leu Gln Ala Ala  Gln Tyr Tyr Arg Thr  Ser Asn Asp 
    1070                 1075                 1080             


Lys Glu  Val Val Asp Arg His  Ile Val Pro Ile Lys  Ser Glu His 
    1085                 1090                 1095             


Asp Tyr  Pro Phe Ala Tyr Thr  Leu Lys Ile Gly Thr  Met Val Leu 
    1100                 1105                 1110             


Leu Tyr  Glu Lys Ser Pro Asn  Glu Val Trp Asp Ala  Thr Ile Lys 
    1115                 1120                 1125             


Glu Arg  Asn Arg Arg Leu Tyr  Lys Val Thr Gly Leu  Ser Thr Met 
    1130                 1135                 1140             


Arg Met  Lys Gly Arg Asn Gly  Glu Tyr Ala Tyr Ala  Thr Val Lys 
    1145                 1150                 1155             


Leu Ile  His Asn Glu Glu Ala  Arg Pro Ser Lys Asp  Ile Lys Ala 
    1160                 1165                 1170             


Lys Asn  Gly Glu Tyr Glu Gln  Gly Glu Glu Phe Arg  Pro Ala Ile 
    1175                 1180                 1185             


Ile Met  Leu His Thr Gln Leu  Asn Ala Leu Val Gln  Gly Tyr Asp 
    1190                 1195                 1200             


Phe Glu  Ile Asn Glu Leu Gly  Glu Ile Arg Arg Leu  Arg 
    1205                 1210                 1215     


<210>  772
<211>  1217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  772

Met Glu Lys Gln Arg Ile Leu Gly Leu Asp Pro Gly Thr Asn Ser Leu 
1               5                   10                  15      


Gly Trp Ala Val Leu Asp Phe Asp Asn Ser Lys Gln Glu Tyr Asn Leu 
            20                  25                  30          


Val Arg Lys Gly Glu Val Ile Phe Glu Glu Gly Val Gly Lys Ser Glu 
        35                  40                  45              


Arg Gly Glu Phe Ser Lys Ala Ala Tyr Arg Thr Phe Tyr Lys Ser Thr 
    50                  55                  60                  


Arg Lys His Tyr Phe Arg Arg Arg Leu Arg Lys Ile Glu Val Leu Lys 
65                  70                  75                  80  


Val Leu Val Lys Tyr Asp Phe Cys Pro Tyr Leu Ser Asp Asp Leu Leu 
                85                  90                  95      


Lys Gln Trp His Glu Lys Lys Ile Tyr Pro Ala Val Lys Glu Phe Met 
            100                 105                 110         


Asp Trp Gln Arg Thr Ser Asp Ala Glu Gly Lys Asn Pro Tyr Tyr Asp 
        115                 120                 125             


Arg His Cys Cys Leu Asn Glu Lys Leu Asp Leu Ser Leu Lys Ser Asp 
    130                 135                 140                 


Arg Tyr Ile Leu Gly Arg Ala Leu Tyr His Leu Thr Gln Arg Arg Gly 
145                 150                 155                 160 


Phe Lys Ser Asn Arg Leu Ile Gln Asn Asn Asp Glu Asn Glu Glu Gly 
                165                 170                 175     


Lys Val Lys Lys Gly Ile Ser Glu Leu Ser Thr Glu Met Gln Glu Ala 
            180                 185                 190         


Asn Cys Asn Tyr Leu Gly Asp Tyr Phe Tyr Lys Leu Tyr Thr Glu Lys 
        195                 200                 205             


Gly Asn Thr Val Gln Ile Arg Lys Arg Tyr Thr Asp Arg Glu Lys His 
    210                 215                 220                 


Tyr Lys Asn Glu Phe Asn Ala Ile Cys Glu Lys Gln Glu Leu Asn Glu 
225                 230                 235                 240 


Thr Ile Val Lys Glu Leu Asp Lys Ala Ile Phe Phe Gln Arg Lys Leu 
                245                 250                 255     


Lys Pro Gln Arg His Gly Val Gly Lys Cys Thr Phe Glu Pro Asn Asn 
            260                 265                 270         


Arg Arg Cys Ala Ile Ser His Pro Asp Phe Glu Arg Phe Arg Met Leu 
        275                 280                 285             


Cys Phe Ile Asn Asn Ile Lys Ile Lys Thr Pro Phe Glu Glu Asn Leu 
    290                 295                 300                 


Arg Pro Leu Asn Asn Glu Glu Lys Ala Leu Ile Glu Pro Leu Phe Tyr 
305                 310                 315                 320 


Arg Lys Ser Ile Lys Asn Phe Asp Phe Glu Glu Ile Ala Lys Lys Leu 
                325                 330                 335     


Ala Gly Lys Asn Asn Tyr Cys Tyr Ile Lys Asp Asp Ser Ser Lys Gln 
            340                 345                 350         


Tyr Lys Phe Asn Tyr Arg Met Ser Gln Gly Ala Ser Thr Cys Gln Thr 
        355                 360                 365             


Ile Ala Lys Leu Lys Ser Ile Phe Gly Glu Glu Trp Lys Asn Gly Ile 
    370                 375                 380                 


Ala Glu Thr Tyr Thr Asn Arg Arg Lys Lys Glu Gly Leu Lys Lys Gln 
385                 390                 395                 400 


Ala Glu Met Val Asp Asp Ile Trp Asn Val Leu Tyr Ser Val Ser Glu 
                405                 410                 415     


Cys Asp Val Leu Lys Asp Phe Ala Ile Asn Lys Leu Gln Leu Asp Glu 
            420                 425                 430         


Lys Thr Ala Asp Asn Phe Ser Lys Thr Lys Leu Ala Gln Gly Phe Ala 
        435                 440                 445             


Thr Leu Ser Leu Lys Ala Ile Arg Lys Ile Leu Pro Phe Leu Glu Gln 
    450                 455                 460                 


Gly Phe Gly Tyr Thr Glu Ala Val Phe Leu Ala Asn Ile Pro Thr Val 
465                 470                 475                 480 


Val Gly Lys Glu Tyr Trp Asn Asn Glu Asp Ile Arg Asn Asp Ile Met 
                485                 490                 495     


Glu Glu Ile Lys Thr Ile Phe Tyr His Asn Thr Leu Lys Thr Asn Asp 
            500                 505                 510         


Ser His Asn Thr Ile Leu Phe Cys Ile Lys Asp Ile Leu Ser Asn Asn 
        515                 520                 525             


Phe Asp Leu Ala Pro Gly Ala Ile Asp Lys Leu Tyr His Pro Ser Met 
    530                 535                 540                 


Ile Asn Val Tyr Lys Asp Val Ile Pro Asn Glu Asp Gly Ile Leu Gln 
545                 550                 555                 560 


Leu Gly Ser Pro Lys Ile Asp Ala Val Lys Asn Pro Met Ala Met Arg 
                565                 570                 575     


Ser Leu His Ile Ile Arg Lys Val Val Asn Glu Leu Leu Arg Glu Gly 
            580                 585                 590         


Ile Ile Asn Ser Asp Thr Glu Val Arg Ile Glu Tyr Ala Arg Glu Leu 
        595                 600                 605             


Asn Asp Ala Asn Lys Arg His Ala Ile Ala Asp Trp Gln Lys Lys Gln 
    610                 615                 620                 


Tyr Gly Lys His Gln Lys Tyr Arg Lys Glu Ile Lys Glu Leu Phe Lys 
625                 630                 635                 640 


Ala Glu Thr Gly Phe Glu Ile Glu Pro Asn Glu Thr Asp Ile Leu Lys 
                645                 650                 655     


Phe Glu Leu Trp Glu Glu Gln Asn His Ile Cys Leu Tyr Thr Gly Gln 
            660                 665                 670         


Glu Ile Gly Ile Ser Glu Phe Val Gly Glu Asn Pro Lys Tyr Asp Ile 
        675                 680                 685             


Glu His Thr Phe Pro Arg Ser Leu Gly Gly Asp Ser Thr Lys Met Asn 
    690                 695                 700                 


Met Thr Leu Cys Glu Asn Lys Phe Asn Arg Glu Asn Lys Gln Lys Lys 
705                 710                 715                 720 


Leu Pro Ser Glu Leu Ala Asn His Ser Glu Ile Leu Glu Arg Ile Asp 
                725                 730                 735     


Asp Trp Lys Lys Lys Ile Tyr Ile Leu Gln Lys Lys Tyr Asp Ser Ile 
            740                 745                 750         


Arg Thr His Asn Gly Met Asp Lys Glu Asn Lys Asp Lys Lys Ile Val 
        755                 760                 765             


Ser Arg His Lys Ile Lys Leu Glu Leu Asp Tyr Trp Lys Gly Lys Tyr 
    770                 775                 780                 


Asn Arg Phe Thr Met Thr Glu Ile Pro Glu Gly Phe Ser Arg Arg Gln 
785                 790                 795                 800 


Gly Met Gly Ile Gly Leu Ile Ser Arg Tyr Ala Gly Leu Tyr Leu Lys 
                805                 810                 815     


Thr Ile Phe His Lys Lys Asp Asn Pro Gln Lys Ser Asn Val Tyr Ile 
            820                 825                 830         


Val Lys Gly Ser Ile Thr Ala Glu Phe Arg Arg Ile Trp Asn Ile Gln 
        835                 840                 845             


Asp Asp Tyr Glu Lys Lys Cys Arg Asp Asn His Ile His His Cys Ile 
    850                 855                 860                 


Asp Ala Ile Val Val Gly Cys Ile Gly Lys Arg Glu Tyr Asp Leu Met 
865                 870                 875                 880 


Ala Gln Phe Tyr Arg Asp Glu Glu Lys Tyr Arg Trp Glu Lys Lys Lys 
                885                 890                 895     


Lys Pro Phe Phe Lys Lys Pro Trp Glu Thr Phe Thr Gln Asp Met Leu 
            900                 905                 910         


Ser Leu Lys Asp Glu Val Leu Thr Val His Phe Asn Pro Ser Asn Phe 
        915                 920                 925             


Thr Lys Lys Ala His Lys Lys Val Phe Thr Pro Lys Gly Ile Phe Val 
    930                 935                 940                 


Ala Gln Gly Asp Cys Ala Arg Val Lys Leu His Lys Gly Ser Tyr Phe 
945                 950                 955                 960 


Gly Ala Ile Glu Gln Lys Gly Glu Ile Lys Tyr Val Met Arg Lys Glu 
                965                 970                 975     


Leu Ser Ala Leu Lys Ile Glu Asp Ile Lys Asn Ile Val Asp Ala Val 
            980                 985                 990         


Val Lys Glu Lys Val Leu Ala Val  Val Lys Lys Lys Gly  Phe Lys Gln 
        995                 1000                 1005             


Ala Met  Ala Glu Pro Ile Tyr  Met Asn Glu Asp Lys  Arg Ile Leu 
    1010                 1015                 1020             


Ile Lys  Lys Val Arg Leu Phe  Val Ser Gln Ala Asn  Pro Leu Ile 
    1025                 1030                 1035             


Val Lys  Lys His Arg Asp Leu  Ser Ser Lys Glu Tyr  Lys Gln Asn 
    1040                 1045                 1050             


Tyr Tyr  Val Asp Asn Glu Gly  Asn Leu Met Ile Ala  Met Tyr Glu 
    1055                 1060                 1065             


Gly Val  Lys Lys Asn Gly Lys  Ile Asp Arg Glu Ile  Thr Val Val 
    1070                 1075                 1080             


Asn Asn  Leu Glu Ala Ala Lys  Phe Phe Arg Gln Ser  Gln Lys Asn 
    1085                 1090                 1095             


Asn Ala  Glu Lys Gln Leu Ile  Ser His Leu Ser Pro  Lys Asn Gly 
    1100                 1105                 1110             


Tyr Pro  Leu Lys Thr Val Leu  Thr Gln Lys Gln Leu  Val Leu Met 
    1115                 1120                 1125             


Tyr Glu  Asp Ser Pro Lys Glu  Ile Lys Leu Arg Asp  Thr Lys Asn 
    1130                 1135                 1140             


Met Val  Lys Arg Leu Tyr Gln  Val Phe Gly Ile Glu  Lys Asp Gly 
    1145                 1150                 1155             


Arg Val  Lys Leu Lys Phe His  Gln Glu Ala Arg Ser  Glu Gly Leu 
    1160                 1165                 1170             


Asn Ile  Asn Ser Ala Ala Phe  Lys Ile Gln Asp Thr  Pro Glu Ser 
    1175                 1180                 1185             


Leu Tyr  Arg His Thr Lys Ser  Asn Leu Lys Val Leu  Val Asn Gly 
    1190                 1195                 1200             


Val Asp  Phe Lys Ile Asn Ile  Leu Gly Glu Ile Ser  Leu Ile 
    1205                 1210                 1215         


<210>  773
<211>  1217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  773

Met Glu Lys Gln Arg Ile Leu Gly Leu Asp Pro Gly Thr Asn Ser Leu 
1               5                   10                  15      


Gly Trp Ala Val Leu Asp Phe Asp Asn Ser Lys Gln Glu Tyr Asn Leu 
            20                  25                  30          


Val Arg Lys Gly Glu Val Ile Phe Glu Glu Gly Val Gly Lys Ser Glu 
        35                  40                  45              


Arg Gly Glu Phe Ser Lys Ala Ala Tyr Arg Thr Phe Tyr Lys Ser Thr 
    50                  55                  60                  


Arg Lys His Tyr Phe Arg Arg Arg Leu Arg Lys Ile Glu Val Leu Lys 
65                  70                  75                  80  


Val Leu Val Lys Tyr Asp Phe Cys Pro Tyr Leu Ser Asp Asp Leu Leu 
                85                  90                  95      


Lys Gln Trp His Glu Lys Lys Ile Tyr Pro Ala Val Lys Glu Phe Met 
            100                 105                 110         


Asp Trp Gln Arg Thr Ser Asp Ala Glu Gly Lys Asn Pro Tyr Tyr Asp 
        115                 120                 125             


Arg His Cys Cys Leu Asn Glu Lys Leu Asp Leu Ser Leu Lys Ser Asp 
    130                 135                 140                 


Arg Tyr Val Leu Gly Arg Ala Leu Tyr His Leu Thr Gln Arg Arg Gly 
145                 150                 155                 160 


Phe Lys Ser Asn Arg Leu Ile Gln Asn Asn Asp Glu Asn Glu Glu Gly 
                165                 170                 175     


Lys Val Lys Lys Gly Ile Ser Glu Leu Ser Thr Glu Met Gln Glu Ala 
            180                 185                 190         


Asn Cys Asn Tyr Leu Gly Asp Tyr Phe Tyr Lys Leu Tyr Thr Glu Lys 
        195                 200                 205             


Gly Asn Thr Val Gln Ile Arg Lys Arg Tyr Thr Asp Arg Glu Lys His 
    210                 215                 220                 


Tyr Lys Asn Glu Phe Asn Ala Ile Cys Glu Lys Gln Glu Leu Asn Glu 
225                 230                 235                 240 


Thr Ile Val Lys Glu Leu Asp Lys Ala Ile Phe Phe Gln Arg Lys Leu 
                245                 250                 255     


Lys Pro Gln Arg His Gly Val Gly Lys Cys Thr Phe Glu Pro Asn Asn 
            260                 265                 270         


Arg Arg Cys Ala Ile Ser His Pro Asp Phe Glu Arg Phe Arg Met Leu 
        275                 280                 285             


Cys Phe Ile Asn Asn Ile Lys Ile Lys Thr Pro Phe Glu Glu Asn Leu 
    290                 295                 300                 


Arg Pro Leu Asn Asn Glu Glu Lys Ala Leu Ile Glu Pro Leu Phe Tyr 
305                 310                 315                 320 


Arg Lys Ser Ile Lys Asn Phe Asp Phe Glu Glu Ile Ala Lys Lys Leu 
                325                 330                 335     


Ala Gly Lys Asn Asn Tyr Cys Tyr Ile Lys Asp Asp Ser Ser Lys Gln 
            340                 345                 350         


Tyr Lys Phe Asn Tyr Arg Met Ser Gln Gly Ala Ser Thr Cys Gln Thr 
        355                 360                 365             


Ile Ala Lys Leu Lys Ser Ile Phe Gly Glu Glu Trp Lys Asn Gly Ile 
    370                 375                 380                 


Ala Glu Thr Tyr Thr Asn Arg Arg Lys Lys Glu Gly Leu Lys Asn Gln 
385                 390                 395                 400 


Ala Glu Met Val Asp Asp Ile Trp Asn Val Leu Tyr Ser Val Ser Glu 
                405                 410                 415     


Cys Asp Val Leu Lys Asp Phe Ala Ile Asn Lys Leu Gln Leu Asp Glu 
            420                 425                 430         


Lys Thr Ala Asp Asn Phe Ser Lys Thr Lys Leu Ala Gln Gly Phe Ala 
        435                 440                 445             


Thr Leu Ser Leu Lys Ala Ile Arg Lys Ile Leu Pro Phe Leu Glu Gln 
    450                 455                 460                 


Gly Phe Gly Tyr Thr Glu Ala Val Phe Leu Ala Asn Ile Pro Thr Val 
465                 470                 475                 480 


Val Gly Lys Glu Tyr Trp Asn Asn Glu Asp Ile Arg Asn Asp Ile Met 
                485                 490                 495     


Glu Glu Ile Lys Thr Ile Phe Tyr His Asn Thr Leu Lys Thr Asn Asp 
            500                 505                 510         


Ser His Asn Thr Ile Leu Phe Cys Ile Lys Asp Ile Leu Ser Asn Asn 
        515                 520                 525             


Phe Asp Leu Ala Pro Gly Ala Ile Asp Lys Leu Tyr His Pro Ser Met 
    530                 535                 540                 


Ile Asn Val Tyr Lys Asp Val Ile Pro Asn Glu Asp Gly Ile Leu Gln 
545                 550                 555                 560 


Leu Gly Ser Pro Lys Ile Asp Ala Val Lys Asn Pro Met Ala Met Arg 
                565                 570                 575     


Ser Leu His Ile Ile Arg Lys Val Val Asn Glu Leu Leu Arg Glu Gly 
            580                 585                 590         


Ile Ile Asn Ser Asp Thr Glu Val Arg Ile Glu Tyr Ala Arg Glu Leu 
        595                 600                 605             


Asn Asp Ala Asn Lys Arg His Ala Ile Ala Asp Trp Gln Lys Lys Gln 
    610                 615                 620                 


Tyr Gly Lys His Gln Lys Tyr Arg Lys Glu Ile Lys Glu Leu Phe Lys 
625                 630                 635                 640 


Ala Glu Thr Gly Phe Glu Ile Glu Pro Asn Glu Thr Asp Ile Leu Lys 
                645                 650                 655     


Phe Glu Leu Trp Glu Glu Gln Asn His Ile Cys Leu Tyr Thr Gly Gln 
            660                 665                 670         


Glu Ile Gly Ile Ser Glu Phe Val Gly Glu Asn Pro Lys Tyr Asp Ile 
        675                 680                 685             


Glu His Thr Ile Pro Arg Ser Leu Gly Gly Asp Ser Thr Lys Met Asn 
    690                 695                 700                 


Met Thr Leu Cys Glu Asn Lys Phe Asn Arg Glu Asn Lys Gln Lys Lys 
705                 710                 715                 720 


Leu Pro Ser Glu Leu Ala Asn His Ser Glu Ile Leu Glu Arg Ile Asp 
                725                 730                 735     


Asp Trp Lys Lys Lys Ile Tyr Ile Leu Gln Lys Lys Tyr Asp Ser Ile 
            740                 745                 750         


Arg Thr His Asn Gly Met Asp Lys Glu Asn Lys Asp Lys Lys Ile Val 
        755                 760                 765             


Ser Arg His Lys Ile Lys Leu Glu Leu Asp Tyr Trp Lys Gly Lys Tyr 
    770                 775                 780                 


Asn Arg Phe Thr Met Thr Glu Ile Pro Glu Gly Phe Ser Arg Arg Gln 
785                 790                 795                 800 


Gly Met Gly Ile Gly Leu Ile Ser Arg Tyr Ala Gly Leu Tyr Leu Lys 
                805                 810                 815     


Thr Ile Phe His Lys Lys Asp Asn Pro Gln Lys Ser Asn Val Tyr Ile 
            820                 825                 830         


Val Lys Gly Ser Ile Thr Ala Glu Phe Arg Arg Ile Trp Asn Ile Gln 
        835                 840                 845             


Asp Asp Tyr Glu Lys Lys Cys Arg Asp Asn His Ile His His Cys Ile 
    850                 855                 860                 


Asp Ala Ile Val Val Gly Cys Ile Gly Lys Arg Glu Tyr Asp Leu Met 
865                 870                 875                 880 


Ala Gln Phe Tyr Arg Asp Glu Glu Lys Tyr Arg Trp Glu Lys Lys Lys 
                885                 890                 895     


Lys Pro Phe Phe Lys Lys Pro Trp Glu Thr Phe Thr Gln Asp Met Leu 
            900                 905                 910         


Ser Leu Lys Asp Glu Val Leu Thr Val His Phe Asn Pro Ser Asn Phe 
        915                 920                 925             


Thr Lys Lys Ala His Lys Lys Val Phe Thr Pro Lys Gly Ile Phe Val 
    930                 935                 940                 


Ala Gln Gly Asp Cys Ala Arg Val Lys Leu His Lys Gly Ser Tyr Phe 
945                 950                 955                 960 


Gly Ala Ile Glu Gln Lys Gly Glu Ile Lys Tyr Val Met Arg Lys Lys 
                965                 970                 975     


Leu Ser Ala Leu Lys Ile Glu Asp Ile Glu His Ile Val Asp Ala Val 
            980                 985                 990         


Val Lys Glu Lys Val Leu Ala Val  Val Lys Lys Lys Gly  Phe Lys Gln 
        995                 1000                 1005             


Ala Met  Ala Glu Pro Ile Tyr  Met Asn Glu Asp Lys  Arg Ile Leu 
    1010                 1015                 1020             


Ile Lys  Lys Val Arg Leu Phe  Val Ser Gln Thr Asn  Pro Leu Ile 
    1025                 1030                 1035             


Val Lys  Lys His Arg Asp Leu  Ser Ser Lys Glu Tyr  Lys Gln Asn 
    1040                 1045                 1050             


Tyr Tyr  Val Asp Asn Glu Gly  Asn Leu Met Ile Ala  Met Tyr Glu 
    1055                 1060                 1065             


Gly Val  Lys Lys Asn Gly Lys  Ile Asp Arg Glu Ile  Thr Val Val 
    1070                 1075                 1080             


Asn Asn  Leu Glu Ala Ala Lys  Phe Phe Arg Gln Ser  Gln Lys Asn 
    1085                 1090                 1095             


Asn Ala  Glu Lys Gln Leu Ile  Ser His Leu Ser Pro  Lys Asn Gly 
    1100                 1105                 1110             


Tyr Pro  Leu Lys Thr Val Leu  Thr Gln Lys Gln Leu  Val Leu Met 
    1115                 1120                 1125             


Tyr Glu  Asp Ser Pro Lys Glu  Ile Lys Leu Arg Asp  Thr Lys Asn 
    1130                 1135                 1140             


Met Val  Lys Arg Leu Tyr Gln  Val Phe Gly Ile Glu  Lys Asp Gly 
    1145                 1150                 1155             


Arg Val  Glu Leu Lys Phe His  Gln Glu Ala Arg Ser  Glu Gly Leu 
    1160                 1165                 1170             


Asn Lys  Asn Ser Ala Ala Phe  Lys Ile Gln Asp Thr  Pro Glu Ser 
    1175                 1180                 1185             


Leu Tyr  Arg His Thr Lys Ser  Asn Leu Lys Val Leu  Val Asn Gly 
    1190                 1195                 1200             


Val Asp  Phe Lys Ile Asn Ile  Leu Gly Glu Ile Ser  Leu Ile 
    1205                 1210                 1215         


<210>  774
<211>  1217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  774

Met Lys Tyr Asn Tyr Val Leu Gly Leu Asp Ile Gly Ile Ser Ser Val 
1               5                   10                  15      


Gly Trp Gly Leu Leu Ala Leu Asp Glu Asn Asn Asn Pro Tyr Lys Ile 
            20                  25                  30          


Ile Asp Val Gly Ser Arg Ile Phe Ser Pro Gly Glu Val Glu Lys Thr 
        35                  40                  45              


Gly Asp Ser Lys Ala Lys Glu Arg Arg Glu Lys Arg Gly Thr Arg Arg 
    50                  55                  60                  


Val Ile Arg Arg Arg Glu Phe Arg Leu Asp Arg Val Arg Asn Leu Leu 
65                  70                  75                  80  


Tyr Glu Lys Gly Tyr Leu Lys Gly Asn Ile Thr Ser Asn Ile Val Ser 
                85                  90                  95      


Val Lys Asn Glu Glu Leu Thr Ile Ile Phe Asn Asp Met Val Asn Asn 
            100                 105                 110         


Tyr Tyr Lys Ser Lys Asp Thr Asn Pro Tyr Lys Leu Lys Val Glu Ala 
        115                 120                 125             


Leu Asp Arg Lys Leu Thr Asn Asp Glu Leu Ser Ile Ile Leu Val His 
    130                 135                 140                 


Tyr Ala Lys Lys Arg Gly Tyr Lys Ser Asn Arg Glu Glu Ser Ser Asp 
145                 150                 155                 160 


Asn Asp Asn Gly Lys Val Lys Glu Ala Ile Lys Glu Asn Thr Asn Leu 
                165                 170                 175     


Met Glu Glu Lys Lys Tyr Arg Thr Ile Ser Glu Met Tyr Ile Lys Asn 
            180                 185                 190         


Glu Lys Phe Lys Asn Lys Ile Lys Asn Gly Pro Asp Asp Tyr Lys Leu 
        195                 200                 205             


Ser Val Thr Asn Glu Met Tyr Leu Glu Glu Ile Asn Lys Val Leu Asp 
    210                 215                 220                 


Ser Gln Ile Gly Phe Gly Leu Ile Asp Asp Asn Phe Lys Asn Glu Tyr 
225                 230                 235                 240 


Leu Glu Ile Tyr Asn Ser Arg Arg His Tyr Ser Glu Gly Pro Gly Tyr 
                245                 250                 255     


Tyr Tyr Ala Lys Asp Ala Asn Gly Asn Lys Val Lys Val Arg Ser Pro 
            260                 265                 270         


Tyr Gly Gly Asp Leu Ile Lys Lys Met Thr Gly Val Cys Ser Phe Asp 
        275                 280                 285             


Lys Lys Ala Arg Ala Pro Lys Tyr Ala Phe Ser Ser Glu Leu Phe Val 
    290                 295                 300                 


Ser Ile Ser Lys Leu Val Asn Leu Arg Tyr Lys Ile Asp Asp Asp Ser 
305                 310                 315                 320 


Tyr Lys Gln Leu Thr Lys Glu Gln Ile Asn Glu Leu Ile Asp Glu Ala 
                325                 330                 335     


Lys Asn Lys Asn Lys Val Thr Tyr Lys Ser Leu Ile Lys Ile Leu Gly 
            340                 345                 350         


Ile Lys Asn Ile Lys Val Lys Asp Leu Asn Leu Ser Lys Ser Glu Tyr 
        355                 360                 365             


Ile Lys Val Ile Asp Glu Leu Lys Lys Lys Leu Asn Ile Ser Lys Glu 
    370                 375                 380                 


Glu Lys Ile Asp Ile Tyr Ser Leu Asp Glu Glu Asn Lys Lys Ile Tyr 
385                 390                 395                 400 


Asp Asp Ile Tyr Asn Glu Lys Leu Phe Ser Lys Thr Leu Ile Glu Phe 
                405                 410                 415     


Lys Gly Tyr Asn Leu Leu Lys Lys Ala Ile Thr Leu Ser Tyr Asn Ser 
            420                 425                 430         


Gly Thr Trp Asn Asp Val Lys Asp Asn Ile Glu Phe Leu Asp Glu Leu 
        435                 440                 445             


Ala Leu Tyr Cys Thr Asp Tyr Lys Val Asn Asn Glu Ile Gln Glu His 
    450                 455                 460                 


Ile His Asn Ser Leu Ile Ile Asp Ser Arg Phe Asp Asp Ser Asn Phe 
465                 470                 475                 480 


Val Glu Asn Leu Pro Asn Phe Lys Asp His Leu Met Leu Ser Thr Asp 
                485                 490                 495     


Ile Ile Arg Lys Leu Ile Pro Ile Met Leu Glu Gly Ile Thr Tyr Asp 
            500                 505                 510         


Lys Ala Met Asp Lys Ile Gly Tyr Asn His Ser Asn Leu Tyr Lys Asp 
        515                 520                 525             


Thr Ile Lys Glu Asp Leu Leu Val Pro Val Tyr Val Ser Lys Asn Ile 
    530                 535                 540                 


Thr Asn Gln Arg Val Ile Arg Ser Leu Thr Gln Ala Arg Lys Val Ile 
545                 550                 555                 560 


Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Lys Ile Ile Asn Ile Glu 
                565                 570                 575     


Thr Ala Arg Glu Leu Ala Lys Thr Gln Lys Glu Arg Arg Ile Ile Glu 
            580                 585                 590         


Lys Asn Gln Gln Asp Lys Gln Tyr Glu Asn Glu Arg Ile Lys Lys His 
        595                 600                 605             


Leu Val Glu Leu Glu Leu Phe Ser Asp Glu Ser Lys Ile Thr Gly Asn 
    610                 615                 620                 


Asp Leu Leu Lys Tyr Lys Leu Trp Gln Glu Gln Lys Glu Phe Cys Gly 
625                 630                 635                 640 


Tyr Ser Met Lys Lys Ile Lys Leu Glu Asp Leu Phe Ser Asn Asn Ile 
                645                 650                 655     


Val Gln Ile Asp His Ile Leu Pro Tyr Ser Arg Thr Tyr Asn Asp Asn 
            660                 665                 670         


Tyr Leu Asn Lys Thr Leu Val Tyr Thr Lys Tyr Asn Gln Asp Lys Gly 
        675                 680                 685             


Asn Lys Thr Pro Tyr Glu Trp Phe Gly Lys Thr Asp Lys Trp Gln Glu 
    690                 695                 700                 


Tyr Glu Ala Phe Ile Asn Ser Leu Ser Ile Ser Gln Asn Lys Lys Asp 
705                 710                 715                 720 


Asn Tyr Leu Leu Lys Asn Leu Asp Gln Asp Ala Glu Arg Glu Met Arg 
                725                 730                 735     


Glu Gln Asn Leu Asn Asp Thr Lys Tyr Ile Ser Arg Glu Leu Leu Ser 
            740                 745                 750         


Leu Ile Lys Ala Tyr Leu Asn Val Asp Glu Val Asn Ala Phe Pro Gly 
        755                 760                 765             


Ala Ile Thr Ala Lys Leu Arg Ala Arg Trp Gly Phe Asn Arg Leu Thr 
    770                 775                 780                 


His Ser Tyr Ile Ser Lys Thr Tyr Ile Met Pro Glu Asp Met Lys Lys 
785                 790                 795                 800 


Gly Ile Asn Lys Asp Arg Asp Asn His Leu His His Ala Met Asp Ala 
                805                 810                 815     


Leu Val Ile Ala Ser Ile Thr Lys Ser Leu Gln Gln Lys Ile Thr Leu 
            820                 825                 830         


Tyr Glu Lys Phe Ser Arg Tyr Val Asp Gly Ile Thr Lys Asn Glu Leu 
        835                 840                 845             


Gly Lys Ile Ser Thr Gly Asn Ile Asn Asp Asp Leu Tyr Asn Tyr Tyr 
    850                 855                 860                 


Asp Glu Glu Thr Gly Glu Ile Lys Glu Leu Glu Leu Arg Gln Tyr Ile 
865                 870                 875                 880 


Lys Asn Gln Val Ala Gln Asn Asn Ile Tyr Phe Ser Lys His Asn Val 
                885                 890                 895     


Ser Lys Leu Ile Phe Pro Thr Pro Tyr Asn Asn Phe Val Glu Glu Ala 
            900                 905                 910         


Lys Leu Arg Val Tyr Glu Arg Asp Leu Glu Val Leu Lys Trp Asn Leu 
        915                 920                 925             


Lys Asn Leu Gly Thr Tyr Thr Ser Ser Glu Leu Asn Arg Ile His Val 
    930                 935                 940                 


Leu Ile Pro Ser Val Ala Lys Asn Lys Ile Ser Gly Arg Leu His Glu 
945                 950                 955                 960 


Glu Thr Tyr Tyr Gly Leu Lys Glu Val Gly Ile Gly Glu Asp Lys Val 
                965                 970                 975     


Thr Tyr Lys Thr Leu Lys Thr Pro Ile Ser Lys Val Lys Lys Lys Asp 
            980                 985                 990         


Leu Asp Asn Ile Pro Asp Cys Lys  Gly Gly Ser Lys Asp  Ile Tyr Asn 
        995                 1000                 1005             


Ala Leu  Val Lys Trp Phe Gly  Asn Cys Glu Thr Gly  Glu Glu Ala 
    1010                 1015                 1020             


Leu Lys  Met His Asp Gly Lys  Tyr Pro Val Asn Pro  Asn Asp Lys 
    1025                 1030                 1035             


Glu Gln  Lys Glu Ile Arg Lys  Ile Lys Val Tyr Thr  Lys Tyr Asn 
    1040                 1045                 1050             


Ala Thr  Gly His Val Val Asn  Asn Gly Asn Val Glu  Lys Gly Gly 
    1055                 1060                 1065             


Ile Tyr  Arg Ile Asp Val Phe  Lys Ser Lys Asn Lys  Glu Asp Asn 
    1070                 1075                 1080             


Lys Leu  Tyr Phe Thr Ser Tyr  Asp Ile Phe Glu Ile  Glu Gln Ile 
    1085                 1090                 1095             


Lys Ala  Tyr Glu Lys Gly Lys  Asn Ile Asn Phe Ser  Ile Lys Leu 
    1100                 1105                 1110             


Glu Tyr  Gly Gln Gly Lys Asn  Asn Lys Ile Leu Leu  Tyr Lys Asp 
    1115                 1120                 1125             


Val Lys  Asn Glu Tyr Asp Leu  Ile Met Ser Ile Tyr  Lys Asn Asp 
    1130                 1135                 1140             


Leu Ile  Arg Ile Lys Thr Lys  Thr Gly Asn Lys Ser  Thr Ala Tyr 
    1145                 1150                 1155             


Val Val  Gly Phe Ser Gly Gly  Lys Leu Glu Ile Lys  Ser Thr Ile 
    1160                 1165                 1170             


Gly Asp  Gly Tyr Asp Ile Ile  Gly Asn Asn Asn Ile  Phe Asp Lys 
    1175                 1180                 1185             


Ser Met  Glu Arg Tyr Arg Val  Thr Ser Ser Thr Ile  Val Lys Ile 
    1190                 1195                 1200             


Asp Lys  Leu Ser Ile Ser Ile  Leu Gly Glu Val Ser  Gly Leu 
    1205                 1210                 1215         


<210>  775
<211>  1218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  775

Met Asn Lys Arg Ile Leu Gly Leu Asp Thr Gly Thr Asn Ser Leu Gly 
1               5                   10                  15      


Trp Ala Val Val Asp Trp Asp Glu His Ala Gln Ser Tyr Glu Leu Ile 
            20                  25                  30          


Lys Tyr Gly Asp Val Ile Phe Gln Glu Gly Val Lys Ile Glu Lys Gly 
        35                  40                  45              


Ile Glu Ser Ser Lys Ala Ala Glu Arg Ser Gly Tyr Lys Ala Ile Arg 
    50                  55                  60                  


Lys Gln Tyr Phe Arg Arg Arg Leu Arg Lys Ile Gln Val Leu Lys Val 
65                  70                  75                  80  


Leu Val Lys Tyr His Leu Cys Pro Tyr Leu Ser Asp Asp Asp Leu Arg 
                85                  90                  95      


Gln Trp His Leu Gln Lys Gln Tyr Pro Lys Ser Asp Glu Leu Met Leu 
            100                 105                 110         


Trp Gln Arg Thr Ser Asp Glu Glu Gly Lys Asn Pro Tyr Tyr Asp Arg 
        115                 120                 125             


His Arg Cys Leu His Glu Lys Leu Asp Leu Thr Val Glu Ala Asp Arg 
    130                 135                 140                 


Tyr Thr Leu Gly Arg Ala Leu Tyr His Leu Thr Gln Arg Arg Gly Phe 
145                 150                 155                 160 


Leu Ser Asn Arg Leu Asp Thr Ser Ala Asp Asn Lys Glu Asp Gly Val 
                165                 170                 175     


Val Lys Ser Gly Ile Ser Gln Leu Ser Thr Glu Met Glu Glu Ala Gly 
            180                 185                 190         


Cys Glu Tyr Leu Gly Asp Tyr Phe Tyr Lys Leu Tyr His Glu Gln Gly 
        195                 200                 205             


Asn Lys Val Arg Ile Arg Gln Arg Tyr Thr Asp Arg Lys Lys His Tyr 
    210                 215                 220                 


Gln His Glu Phe Asp Ala Ile Cys Glu Lys Gln Glu Leu Ser Ser Glu 
225                 230                 235                 240 


Leu Ile Glu Asp Leu Gln Arg Ala Ile Phe Phe Gln Leu Pro Leu Lys 
                245                 250                 255     


Ser Gln Arg His Gly Val Gly Arg Cys Thr Phe Glu Arg Gly Lys Pro 
            260                 265                 270         


Arg Cys Ala Asp Ser His Pro Asp Tyr Glu Glu Phe Arg Met Leu Cys 
        275                 280                 285             


Phe Val Asn Asn Ile Gln Val Lys Gly Pro His Asp Leu Glu Leu Arg 
    290                 295                 300                 


Pro Leu Thr Tyr Glu Glu Arg Glu Lys Ile Glu His Leu Phe Phe Arg 
305                 310                 315                 320 


Lys Ser Lys Pro Asn Phe Asp Phe Glu Asp Ile Ala Lys Glu Leu Ala 
                325                 330                 335     


Gly Lys Lys Asn Tyr Ala Trp Ile His Asp Lys Glu Glu Arg Ala Tyr 
            340                 345                 350         


Lys Phe Asn Tyr Arg Met Thr Gln Gly Val Pro Gly Cys Pro Thr Thr 
        355                 360                 365             


Ala Gln Leu Lys Ser Ile Phe Gly Asp Asp Trp Lys Thr Gly Ile Ala 
    370                 375                 380                 


Glu Thr Tyr Thr Leu Ile Gln Lys Lys Asn Gly Ser Lys Ser Leu Gln 
385                 390                 395                 400 


Glu Met Val Asp Asp Val Trp Asn Val Leu Tyr Ser Phe Ser Ser Val 
                405                 410                 415     


Glu Lys Leu Lys Glu Phe Ala His His Lys Leu Gln Leu Asp Glu Glu 
            420                 425                 430         


Asn Ala Glu Lys Phe Ala Lys Ile Lys Leu Ser His Ser Phe Ala Ala 
        435                 440                 445             


Leu Ser Leu Lys Ala Ile Arg Lys Phe Leu Pro Phe Leu Arg Lys Gly 
    450                 455                 460                 


Met Tyr Tyr Thr His Ala Ser Phe Phe Ala Asn Ile Pro Thr Ile Val 
465                 470                 475                 480 


Gly Lys Glu Ile Trp Asn Asn Glu Gln Ser Arg Lys Tyr Ile Met Glu 
                485                 490                 495     


Asn Val Gly Glu Leu Val Phe Asn Tyr Gln Pro Lys His Arg Asp Val 
            500                 505                 510         


Gln Gly Thr Ile Glu Met Leu Ile Lys Asp Phe Leu Ala Asn Asn Phe 
        515                 520                 525             


Glu Leu Pro Ala Gly Ala Thr Asp Lys Leu Tyr His Pro Ser Met Ile 
    530                 535                 540                 


Glu Thr Tyr Pro His Ala Leu Arg Asn Glu Phe Gly Ile Leu Gln Leu 
545                 550                 555                 560 


Gly Ser Pro Arg Thr Asn Ala Ile Arg Asn Pro Met Ala Met Arg Ser 
                565                 570                 575     


Leu His Val Leu Arg Arg Val Val Asn Gln Leu Leu Lys Glu Ser Ile 
            580                 585                 590         


Ile Asp Glu Asn Thr Glu Val His Val Glu Tyr Ala Arg Glu Leu Asn 
        595                 600                 605             


Asp Ala Asn Lys Arg Arg Ala Ile Ala Asp Arg Gln Lys Glu Gln Asp 
    610                 615                 620                 


Lys Gln His Lys Lys Tyr Gly Asp Glu Ile Arg Lys Leu Tyr Lys Glu 
625                 630                 635                 640 


Glu Thr Gly Lys Asp Ile Glu Pro Thr Gln Thr Asp Val Leu Lys Phe 
                645                 650                 655     


Gln Leu Trp Glu Glu Gln Asn His Tyr Cys Leu Tyr Thr Gly Glu Gln 
            660                 665                 670         


Ile Gly Ile Thr Asp Phe Ile Gly Ser Asn Pro Lys Phe Asp Ile Glu 
        675                 680                 685             


His Thr Ile Pro Gln Ser Val Gly Gly Asp Ser Thr Gln Met Asn Leu 
    690                 695                 700                 


Thr Leu Cys Asp Asn Arg Phe Asn Arg Glu Val Lys Lys Ala Lys Leu 
705                 710                 715                 720 


Pro Thr Glu Leu Ala Asn His Glu Glu Ile Leu Thr Arg Ile Glu Pro 
                725                 730                 735     


Trp Lys Asn Lys Tyr Glu Gln Leu Val Lys Glu Arg Asp Lys Gln Arg 
            740                 745                 750         


Thr Phe Ala Gly Met Asp Lys Ala Val Lys Asp Ile Arg Ile Gln Lys 
        755                 760                 765             


Arg His Lys Leu Gln Met Glu Ile Asp Tyr Trp Arg Gly Lys Tyr Glu 
    770                 775                 780                 


Arg Phe Thr Met Thr Glu Val Pro Glu Gly Phe Ser Arg Arg Gln Gly 
785                 790                 795                 800 


Thr Gly Ile Gly Leu Ile Ser Arg Tyr Ala Gly Leu Tyr Leu Lys Ser 
                805                 810                 815     


Leu Phe His Gln Ala Asp Ser Arg Asn Lys Ser Asn Val Tyr Val Val 
            820                 825                 830         


Lys Gly Val Ala Thr Ala Glu Phe Arg Lys Met Trp Gly Leu Gln Ser 
        835                 840                 845             


Glu Tyr Glu Lys Lys Cys Arg Asp Asn His Ser His His Cys Met Asp 
    850                 855                 860                 


Ala Ile Thr Ile Ala Cys Ile Gly Lys Arg Glu Tyr Asp Leu Met Ala 
865                 870                 875                 880 


Glu Tyr Tyr His Met Glu Glu Thr Phe Arg Gln Gly Arg Gly Ser Lys 
                885                 890                 895     


Pro Lys Phe Ser Lys Pro Trp Ala Thr Phe Thr Glu Asp Val Leu Asn 
            900                 905                 910         


Ile Tyr Lys Asn Leu Leu Val Val His Asp Thr Pro Asn Asn Met Pro 
        915                 920                 925             


Lys His Thr Lys Lys Tyr Val Gln Thr Ser Lys Gly Lys Val Leu Ala 
    930                 935                 940                 


Gln Gly Asp Thr Ala Arg Gly Ser Leu His Leu Asp Thr Tyr Tyr Gly 
945                 950                 955                 960 


Ala Ile Glu Arg Asp Gly Glu Ile Arg Tyr Val Val Arg Arg Pro Leu 
                965                 970                 975     


Ser Ser Phe Thr Asn Pro Lys Glu Leu Glu Asn Ile Val Asp Glu Thr 
            980                 985                 990         


Val Lys Arg Thr Ile Lys Glu Ala  Ile Ala Asp Lys Asn  Phe Lys Gln 
        995                 1000                 1005             


Ala Ile  Ala Glu Pro Ile Tyr  Met Asn Glu Asp Lys  Gly Ile Leu 
    1010                 1015                 1020             


Ile Lys  Lys Val Arg Cys Phe  Ala Asn Ser Val Lys  Gln Pro Ile 
    1025                 1030                 1035             


Asn Ile  Arg Gln His Arg Asp  Leu Ser Lys Lys Glu  Tyr Lys Gln 
    1040                 1045                 1050             


Gln Tyr  His Val Met Asn Glu  Asn Asn Tyr Leu Leu  Ala Ile Tyr 
    1055                 1060                 1065             


Glu Gly  Leu Val Lys Asn Lys  Val Val Arg Glu Phe  Glu Ile Val 
    1070                 1075                 1080             


Ser Tyr  Ile Glu Ala Ala Lys  Tyr Tyr Lys Arg Ser  Gln Asp Arg 
    1085                 1090                 1095             


Asn Ile  Phe Ser Ser Ile Val  Pro Thr His Ser Thr  Lys Tyr Gly 
    1100                 1105                 1110             


Leu Pro  Leu Lys Thr Lys Leu  Leu Met Gly Gln Gln  Val Leu Met 
    1115                 1120                 1125             


Phe Glu  Glu Asn Pro Asp Glu  Ile Gln Val Asp Asn  Thr Lys Asp 
    1130                 1135                 1140             


Leu Val  Lys Arg Leu Tyr Lys  Val Val Gly Ile Glu  Lys Asp Gly 
    1145                 1150                 1155             


Arg Ile  Lys Phe Lys His His  Gln Glu Ala Arg Lys  Glu Gly Leu 
    1160                 1165                 1170             


Thr Ile  Tyr Ser Thr Pro Tyr  Lys Asn Asn Asp Asp  Tyr Val Pro 
    1175                 1180                 1185             


Ile Phe  Arg Gln Ser Ile Lys  Asn Ile Asn Ile Leu  Val Asp Gly 
    1190                 1195                 1200             


Ile Asp  Phe Asn Ile Asp Ile  Leu Gly Lys Val Thr  Leu Lys Glu 
    1205                 1210                 1215             


<210>  776
<211>  1218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  776

Met Asn Lys Arg Ile Leu Gly Leu Asp Thr Gly Thr Asn Ser Leu Gly 
1               5                   10                  15      


Trp Ala Val Val Asp Trp Asp Glu His Ala Gln Ser Tyr Glu Leu Ile 
            20                  25                  30          


Lys Tyr Gly Asp Val Ile Phe Gln Glu Gly Val Lys Ile Glu Lys Gly 
        35                  40                  45              


Ile Glu Ser Ser Lys Ala Ala Glu Arg Ser Gly Tyr Lys Ala Ile Arg 
    50                  55                  60                  


Lys Gln Tyr Phe Arg Arg Arg Leu Arg Lys Ile Gln Val Leu Lys Val 
65                  70                  75                  80  


Leu Val Lys Tyr His Leu Cys Pro Tyr Leu Ser Asp Asp Asp Leu Arg 
                85                  90                  95      


Gln Trp His Leu Gln Lys Gln Tyr Pro Lys Ser Asp Glu Leu Met Leu 
            100                 105                 110         


Trp Gln Arg Thr Ser Asp Glu Glu Gly Lys Asn Pro Tyr Tyr Asp Arg 
        115                 120                 125             


His Arg Cys Leu His Glu Lys Leu Asp Leu Thr Val Glu Ala Asp Arg 
    130                 135                 140                 


Tyr Thr Leu Gly Arg Ala Leu Tyr His Leu Thr Gln Arg Arg Gly Phe 
145                 150                 155                 160 


Leu Ser Asn Arg Leu Asp Thr Ser Ala Asp Asn Lys Glu Asp Gly Val 
                165                 170                 175     


Val Lys Ser Gly Ile Ser Gln Leu Ser Thr Glu Met Glu Glu Ala Gly 
            180                 185                 190         


Cys Glu Tyr Leu Gly Asp Tyr Phe Tyr Lys Leu Tyr Asp Ala Gln Gly 
        195                 200                 205             


Asn Lys Val Arg Ile Arg Gln Arg Tyr Thr Asp Arg Asn Lys His Tyr 
    210                 215                 220                 


Gln His Glu Phe Asp Ala Ile Cys Glu Lys Gln Glu Leu Ser Ser Glu 
225                 230                 235                 240 


Leu Ile Glu Asp Leu Gln Arg Ala Ile Phe Phe Gln Leu Pro Leu Lys 
                245                 250                 255     


Ser Gln Arg His Gly Val Gly Arg Cys Thr Phe Glu Arg Gly Lys Pro 
            260                 265                 270         


Arg Cys Ala Asp Ser His Pro Asp Tyr Glu Glu Phe Arg Met Leu Cys 
        275                 280                 285             


Phe Val Asn Asn Ile Gln Val Lys Gly Pro His Asp Leu Glu Leu Arg 
    290                 295                 300                 


Pro Leu Thr Tyr Glu Glu Arg Glu Lys Ile Glu Pro Leu Phe Phe Arg 
305                 310                 315                 320 


Lys Ser Lys Pro Asn Phe Asp Phe Glu Asp Ile Ala Lys Ala Leu Ala 
                325                 330                 335     


Gly Lys Lys Asn Tyr Ala Trp Ile His Asp Lys Glu Glu Arg Ala Tyr 
            340                 345                 350         


Lys Phe Asn Tyr Arg Met Thr Gln Gly Val Pro Gly Cys Pro Thr Ile 
        355                 360                 365             


Ala Gln Leu Lys Ser Ile Phe Gly Asp Asp Trp Lys Thr Gly Ile Ala 
    370                 375                 380                 


Glu Thr Tyr Thr Leu Ile Gln Lys Lys Asn Gly Ser Lys Ser Leu Gln 
385                 390                 395                 400 


Glu Met Val Asp Asp Val Trp Asn Val Leu Tyr Ser Phe Ser Ser Val 
                405                 410                 415     


Glu Lys Leu Lys Glu Phe Ala His His Lys Leu Gln Leu Asp Glu Glu 
            420                 425                 430         


Ser Ala Glu Lys Phe Ala Lys Ile Lys Leu Ser His Ser Phe Ala Ala 
        435                 440                 445             


Leu Ser Leu Lys Ala Ile Arg Lys Phe Leu Pro Phe Leu Arg Lys Gly 
    450                 455                 460                 


Met Tyr Tyr Thr His Ala Ser Phe Phe Ala Asn Ile Pro Thr Ile Val 
465                 470                 475                 480 


Gly Lys Glu Ile Trp Asn Lys Glu Gln Asn Arg Lys Tyr Ile Met Glu 
                485                 490                 495     


Asn Val Gly Glu Leu Val Phe Asn Tyr Gln Pro Lys His Arg Glu Val 
            500                 505                 510         


Gln Gly Thr Ile Glu Met Leu Ile Lys Asp Phe Leu Ala Asn Asn Phe 
        515                 520                 525             


Glu Leu Pro Ala Gly Ala Thr Asp Lys Leu Tyr His Pro Ser Met Ile 
    530                 535                 540                 


Glu Thr Tyr Pro Asn Ala Gln Arg Asn Glu Phe Gly Ile Leu Gln Leu 
545                 550                 555                 560 


Gly Ser Pro Arg Thr Asn Ala Ile Arg Asn Pro Met Ala Met Arg Ser 
                565                 570                 575     


Leu His Ile Leu Arg Arg Val Val Asn Gln Leu Leu Lys Glu Ser Ile 
            580                 585                 590         


Ile Asp Glu Asn Thr Glu Val His Val Glu Tyr Ala Arg Glu Leu Asn 
        595                 600                 605             


Asp Ala Asn Lys Arg Arg Ala Ile Ala Asp Arg Gln Lys Glu Gln Asp 
    610                 615                 620                 


Lys Gln His Lys Lys Tyr Gly Asp Glu Ile Arg Lys Leu Tyr Lys Glu 
625                 630                 635                 640 


Glu Thr Gly Lys Asp Ile Glu Pro Thr Gln Thr Asp Val Leu Lys Phe 
                645                 650                 655     


Gln Leu Trp Glu Glu Gln Asn His His Cys Leu Tyr Thr Gly Glu Gln 
            660                 665                 670         


Ile Gly Ile Thr Asp Phe Ile Gly Ser Asn Pro Lys Phe Asp Ile Glu 
        675                 680                 685             


His Thr Ile Pro Gln Ser Val Gly Gly Asp Ser Thr Gln Met Asn Leu 
    690                 695                 700                 


Thr Leu Cys Asp Asn Arg Phe Asn Arg Glu Val Lys Lys Ala Lys Leu 
705                 710                 715                 720 


Pro Thr Glu Leu Ala Asn His Glu Glu Ile Leu Thr Arg Ile Glu Pro 
                725                 730                 735     


Trp Lys Asn Lys Tyr Glu Gln Leu Val Lys Glu Arg Asp Lys Gln Arg 
            740                 745                 750         


Thr Phe Ala Gly Met Asp Lys Ala Val Lys Asp Ile Arg Ile Gln Lys 
        755                 760                 765             


Arg His Lys Leu Gln Met Glu Ile Asp Tyr Trp Arg Gly Lys Tyr Glu 
    770                 775                 780                 


Arg Phe Thr Met Thr Glu Val Pro Glu Gly Phe Ser Arg Arg Gln Gly 
785                 790                 795                 800 


Thr Gly Ile Gly Leu Ile Ser Arg Tyr Ala Gly Leu Tyr Leu Lys Ser 
                805                 810                 815     


Leu Phe His Gln Ala Asp Ser Arg Asn Lys Ser Asn Val Tyr Val Val 
            820                 825                 830         


Lys Gly Val Ala Thr Ala Glu Phe Arg Lys Met Trp Gly Leu Gln Ser 
        835                 840                 845             


Glu Tyr Glu Lys Lys Cys Arg Asp Asn His Ser His His Cys Met Asp 
    850                 855                 860                 


Ala Ile Thr Ile Ala Cys Ile Gly Lys Arg Glu Tyr Asp Leu Met Ala 
865                 870                 875                 880 


Glu Tyr Tyr Arg Met Glu Glu Thr Phe Lys Gln Gly Arg Gly Ser Lys 
                885                 890                 895     


Pro Lys Phe Ser Lys Pro Trp Ala Thr Phe Thr Glu Asp Val Leu Asn 
            900                 905                 910         


Ile Tyr Lys Asn Leu Leu Val Val His Asp Thr Pro Asn Asn Met Pro 
        915                 920                 925             


Lys His Thr Lys Lys Tyr Val Gln Thr Ser Ile Gly Lys Val Leu Ala 
    930                 935                 940                 


Gln Gly Asp Thr Ala Arg Gly Ser Leu His Leu Asp Thr Tyr Tyr Gly 
945                 950                 955                 960 


Ala Ile Glu Arg Asp Gly Glu Ile Arg Tyr Val Val Arg Arg Pro Leu 
                965                 970                 975     


Ser Ser Phe Thr Lys Pro Glu Glu Leu Glu Asn Ile Val Asp Glu Thr 
            980                 985                 990         


Val Lys Arg Thr Ile Lys Glu Ala  Ile Ala Asp Lys Asn  Phe Lys Gln 
        995                 1000                 1005             


Ala Ile  Ala Glu Pro Ile Tyr  Met Asn Glu Glu Lys  Gly Ile Leu 
    1010                 1015                 1020             


Ile Lys  Lys Val Arg Cys Phe  Ala Lys Ser Val Lys  Gln Pro Ile 
    1025                 1030                 1035             


Asn Ile  Arg Gln His Arg Asp  Leu Ser Lys Lys Glu  Tyr Lys Gln 
    1040                 1045                 1050             


Gln Tyr  His Val Met Asn Glu  Asn Asn Tyr Leu Leu  Ala Ile Tyr 
    1055                 1060                 1065             


Glu Gly  Leu Val Lys Asn Lys  Val Val Arg Glu Phe  Glu Ile Val 
    1070                 1075                 1080             


Ser Tyr  Ile Glu Ala Ala Lys  Tyr Tyr Lys Arg Ser  Gln Asp Arg 
    1085                 1090                 1095             


Asn Ile  Phe Ser Ser Ile Val  Pro Thr His Ser Thr  Lys Tyr Gly 
    1100                 1105                 1110             


Leu Pro  Leu Lys Thr Lys Leu  Leu Met Gly Gln Leu  Val Leu Met 
    1115                 1120                 1125             


Phe Glu  Glu Asn Pro Asp Glu  Ile Gln Val Asp Asn  Thr Lys Asp 
    1130                 1135                 1140             


Leu Val  Lys Arg Leu Tyr Lys  Val Val Gly Ile Glu  Lys Asp Gly 
    1145                 1150                 1155             


Arg Ile  Lys Phe Lys Tyr His  Gln Glu Ala Arg Lys  Glu Gly Leu 
    1160                 1165                 1170             


Pro Ile  Phe Ser Thr Pro Tyr  Lys Asn Asn Asp Asp  Tyr Ala Pro 
    1175                 1180                 1185             


Ile Phe  Arg Gln Ser Ile Asn  Asn Ile Asn Ile Leu  Val Asp Gly 
    1190                 1195                 1200             


Ile Asp  Phe Thr Ile Asp Ile  Leu Gly Lys Val Thr  Leu Lys Glu 
    1205                 1210                 1215             


<210>  777
<211>  1218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  777

Met Asn Lys Arg Ile Leu Gly Leu Asp Thr Gly Thr Asn Ser Leu Gly 
1               5                   10                  15      


Trp Ala Val Val Asp Trp Asp Glu His Ala Gln Ser Tyr Glu Phe Ile 
            20                  25                  30          


Lys Tyr Gly Asp Val Ile Phe Gln Glu Gly Val Lys Ile Glu Lys Gly 
        35                  40                  45              


Ile Glu Ser Ser Lys Ala Ala Glu Arg Ser Gly Tyr Lys Ala Ile Arg 
    50                  55                  60                  


Lys Gln Tyr Phe Arg Arg Arg Leu Arg Lys Ile Gln Val Leu Lys Val 
65                  70                  75                  80  


Leu Val Lys Tyr His Leu Cys Pro Tyr Leu Ser Asp Asp Asp Leu Arg 
                85                  90                  95      


Gln Trp His Leu Gln Lys Gln Tyr Pro Lys Ser Asp Glu Leu Met Leu 
            100                 105                 110         


Trp Gln Arg Thr Ser Asp Lys Lys Gly Lys Asn Pro Tyr Tyr Asp Arg 
        115                 120                 125             


His Arg Cys Leu His Glu Lys Leu Asp Leu Thr Val Glu Ala Asp Arg 
    130                 135                 140                 


Tyr Thr Leu Gly Arg Ala Leu Tyr His Leu Thr Gln Arg Arg Gly Phe 
145                 150                 155                 160 


Leu Ser Asn Arg Leu Asp Thr Ser Ala Asp Asn Lys Glu Asp Gly Val 
                165                 170                 175     


Val Lys Ser Gly Ile Ser Gln Leu Ser Thr Glu Met Glu Glu Ala Gly 
            180                 185                 190         


Cys Glu Tyr Leu Gly Asp Tyr Phe Tyr Lys Leu Tyr Asp Glu Gln Gly 
        195                 200                 205             


Asn Lys Val Arg Ile Arg Gln Arg Tyr Thr Asp Arg Asn Lys His Tyr 
    210                 215                 220                 


Gln His Glu Phe Asp Ala Ile Cys Glu Lys Gln Glu Leu Ser Ser Glu 
225                 230                 235                 240 


Leu Ile Glu Asp Leu Gln Arg Ala Ile Phe Phe Gln Leu Pro Leu Lys 
                245                 250                 255     


Ser Gln Arg His Gly Val Gly Arg Cys Thr Phe Glu Arg Gly Lys Pro 
            260                 265                 270         


Arg Cys Ala Asp Ser His Pro Asp Tyr Glu Glu Phe Arg Met Leu Cys 
        275                 280                 285             


Phe Val Asn Asn Ile Gln Val Lys Gly Pro His Asp Leu Glu Leu Arg 
    290                 295                 300                 


Pro Leu Thr Tyr Glu Glu Arg Glu Lys Ile Glu Pro Leu Phe Phe Arg 
305                 310                 315                 320 


Lys Ser Lys Pro Asn Phe Asp Phe Glu Asp Ile Ala Lys Ala Leu Ala 
                325                 330                 335     


Gly Lys Lys Asn Tyr Ala Trp Ile His Asp Lys Glu Glu Arg Ala Tyr 
            340                 345                 350         


Lys Phe Asn Tyr Arg Met Thr Gln Gly Val Pro Gly Cys Pro Thr Thr 
        355                 360                 365             


Ala Gln Leu Lys Ser Ile Phe Gly Asp Asp Trp Lys Thr Gly Ile Ala 
    370                 375                 380                 


Glu Thr Tyr Thr Leu Ile Gln Lys Lys Asn Gly Ser Lys Ser Leu Gln 
385                 390                 395                 400 


Glu Met Glu Asp Asp Val Trp Asn Val Leu Tyr Ser Phe Ser Ser Val 
                405                 410                 415     


Glu Lys Leu Lys Glu Phe Ala His His Lys Leu Gln Leu Asp Glu Glu 
            420                 425                 430         


Ser Ala Glu Lys Phe Ala Lys Ile Lys Leu Ser His Ser Phe Ala Ala 
        435                 440                 445             


Leu Ser Leu Lys Ala Ile Arg Lys Phe Leu Pro Phe Leu Arg Lys Gly 
    450                 455                 460                 


Met Tyr Tyr Thr His Ala Ser Phe Phe Ala Asn Ile Pro Thr Ile Val 
465                 470                 475                 480 


Gly Lys Glu Ile Trp Asn Asn Glu Gln Asn Arg Lys Tyr Ile Met Glu 
                485                 490                 495     


Asn Val Gly Glu Leu Val Phe Asn Tyr Gln Pro Lys His Arg Glu Val 
            500                 505                 510         


Gln Gly Thr Ile Glu Met Leu Ile Lys Asp Phe Leu Ala Asn Asn Phe 
        515                 520                 525             


Glu Leu Pro Ala Gly Ala Thr Asp Lys Leu Tyr His Pro Ser Met Ile 
    530                 535                 540                 


Glu Thr Tyr Pro His Ala Gln Arg Asn Glu Phe Gly Ile Leu Gln Leu 
545                 550                 555                 560 


Gly Ser Pro Arg Thr Asn Ala Ile Arg Asn Pro Met Ala Met Arg Ser 
                565                 570                 575     


Leu His Ile Leu Arg Arg Val Val Asn Gln Leu Leu Lys Glu Ser Ile 
            580                 585                 590         


Ile Asp Glu Asn Thr Glu Val His Val Glu Tyr Ala Arg Glu Leu Asn 
        595                 600                 605             


Asp Ala Asn Lys Arg Arg Ala Ile Ala Gly Trp Gln Lys Glu Gln Asp 
    610                 615                 620                 


Lys Gln His Lys Lys Tyr Val Asp Glu Ile Arg Lys Leu Tyr Lys Glu 
625                 630                 635                 640 


Glu Thr Gly Lys Asp Ile Glu Pro Thr Gln Thr Asp Val Leu Lys Phe 
                645                 650                 655     


Gln Leu Trp Glu Glu Gln Asp His His Cys Leu Tyr Thr Gly Glu Gln 
            660                 665                 670         


Ile Gly Ile Thr Asp Phe Ile Gly Ser Asn Pro Lys Phe Asp Ile Glu 
        675                 680                 685             


His Thr Ile Pro Gln Ser Val Gly Gly Asp Ser Thr Gln Met Asn Leu 
    690                 695                 700                 


Thr Leu Cys Asp Asn Arg Phe Asn Arg Glu Ile Lys Lys Ala Lys Leu 
705                 710                 715                 720 


Pro Ala Gln Leu Ser Asn His Glu Glu Ile Leu Ala Arg Ile Glu Pro 
                725                 730                 735     


Trp Lys Asn Lys Tyr Glu Gln Lys Ile Lys Glu Arg Asp Lys Gln Arg 
            740                 745                 750         


Thr Phe Ala Gly Met Asp Lys Ala Val Lys Asp Leu Arg Ile Gln Lys 
        755                 760                 765             


Arg His Lys Leu Gln Met Asp Ile Asp Tyr Trp Arg Gly Lys Tyr Glu 
    770                 775                 780                 


Arg Phe Thr Met Thr Glu Val Pro Glu Gly Phe Ser Arg Arg Gln Gly 
785                 790                 795                 800 


Thr Gly Ile Gly Leu Ile Ser Arg Tyr Ala Gly Leu Tyr Leu Lys Ser 
                805                 810                 815     


Leu Phe His Gln Ala Asp Ser Arg Asn Lys Ser Asn Val Tyr Val Val 
            820                 825                 830         


Lys Gly Val Ala Thr Ala Glu Phe Arg Lys Met Trp Gly Leu Gln Ser 
        835                 840                 845             


Glu Tyr Glu Lys Lys Cys Arg Asp Asn His Ser His His Cys Met Asp 
    850                 855                 860                 


Ala Ile Thr Ile Ala Cys Ile Gly Lys Arg Glu Tyr Asp Leu Met Ala 
865                 870                 875                 880 


Glu Tyr Tyr Arg Met Glu Glu Thr Phe Arg Gln Gly Arg Gly Ser Lys 
                885                 890                 895     


Pro Lys Phe Ser Lys Pro Trp Ala Thr Phe Thr Glu Asp Val Leu Asn 
            900                 905                 910         


Ile Tyr Lys Asn Leu Leu Val Val His Asp Thr Pro Asn Asn Met Pro 
        915                 920                 925             


Lys His Thr Lys Lys Tyr Val Gln Thr Ser Lys Gly Lys Val Leu Ala 
    930                 935                 940                 


Gln Gly Asp Thr Ala Arg Gly Ser Leu His Leu Asp Thr Tyr Tyr Gly 
945                 950                 955                 960 


Ala Ile Glu Arg Asp Gly Glu Ile Arg Tyr Val Val Arg Arg Pro Leu 
                965                 970                 975     


Ser Ser Phe Thr Lys Pro Lys Glu Leu Glu Asn Ile Val Asp Glu Thr 
            980                 985                 990         


Val Lys Arg Thr Ile Lys Glu Ala  Ile Ala Asp Lys Asn  Phe Lys Gln 
        995                 1000                 1005             


Ala Ile  Ala Glu Pro Ile Tyr  Met Asn Glu Asp Lys  Gly Ile Leu 
    1010                 1015                 1020             


Ile Lys  Lys Val Arg Cys Phe  Ala Lys Ser Val Lys  Gln Pro Ile 
    1025                 1030                 1035             


Asn Ile  Arg Gln His Arg Asp  Leu Ser Lys Lys Glu  Tyr Lys Gln 
    1040                 1045                 1050             


Gln Tyr  His Val Val Asn Glu  Asn Asn Tyr Met Leu  Val Ile Tyr 
    1055                 1060                 1065             


Glu Gly  Leu Val Lys Asn Lys  Val Val Arg Glu Phe  Glu Ile Val 
    1070                 1075                 1080             


Ser Tyr  Ile Glu Ala Ala Lys  Tyr Tyr Lys Arg Ser  Gln Asp Arg 
    1085                 1090                 1095             


Asn Ile  Phe Ser Ser Ile Val  Pro Thr His Ser Ala  Lys Tyr Gly 
    1100                 1105                 1110             


Leu Pro  Leu Lys Thr Lys Leu  Leu Met Gly Gln Leu  Val Leu Met 
    1115                 1120                 1125             


Phe Glu  Glu Asn Pro Asp Glu  Ile Gln Val Asp Asn  Thr Lys Asp 
    1130                 1135                 1140             


Leu Val  Lys Arg Leu Tyr Lys  Val Val Gly Ile Lys  Lys Asp Gly 
    1145                 1150                 1155             


Arg Val  Lys Phe Lys Phe His  Gln Glu Ala Arg Lys  Asp Gly Leu 
    1160                 1165                 1170             


Leu Ile  Phe Ser Thr Pro Tyr  Lys Asn Asn Asn Gly  Tyr Val Pro 
    1175                 1180                 1185             


Ile Phe  Arg His Ser Lys Lys  Asn Ile Asn Ile Leu  Val Asp Gly 
    1190                 1195                 1200             


Lys Asp  Phe Thr Ile Asp Ile  Leu Gly Lys Val Thr  Leu Lys Glu 
    1205                 1210                 1215             


<210>  778
<211>  1219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  778

Met Lys Glu Glu Val Arg Gly Lys Asn Arg Ser Gln Thr Trp Ala Ile 
1               5                   10                  15      


Leu Ala Lys Phe Phe Val Trp Ser Glu Lys Glu Ser Val Trp Asn Glu 
            20                  25                  30          


Lys Glu Ala Arg Thr Glu Glu Arg Val Lys Glu Tyr Lys Leu Val Gly 
        35                  40                  45              


Leu Lys Arg Asp Thr Lys Gly Asn Glu Leu Lys Leu Glu Asn Tyr Arg 
    50                  55                  60                  


Trp Arg Val Gln Ala Leu Ser Glu Gln Leu Asn Pro Glu Ile Val Ala 
65                  70                  75                  80  


Ile Val Leu Gln Glu Ile Asn Gly Gln Ile Ser Ala Ser Ser Gly Tyr 
                85                  90                  95      


Leu Gly Ala Ile Ser Asp Arg Ser Lys Gln Leu Tyr Phe Asn Arg Gln 
            100                 105                 110         


Thr Val Gly Gln Tyr Leu Met Ser Glu Leu Glu Lys Asp Pro Asn Val 
        115                 120                 125             


Ser Leu Arg Asn Arg Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe 
    130                 135                 140                 


Asp Arg Ile Trp Glu Lys Gln Ala Glu Phe His Glu Glu Leu Thr Val 
145                 150                 155                 160 


Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Arg 
                165                 170                 175     


Leu Lys Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Lys Arg 
            180                 185                 190         


Glu Val Val Val Glu Glu Asp Gly Arg Lys Lys Asn Lys Thr Val Gly 
        195                 200                 205             


Cys Arg Val Ile Pro Arg Ser His Pro Leu Phe Gln Glu Phe Lys Ile 
    210                 215                 220                 


Trp Gln Thr Leu Asn Asp Ile Glu Val Phe Ala Trp Asp Lys Gln Ser 
225                 230                 235                 240 


Lys Arg Lys Lys Ala Asp Lys Ser Ser Ile Leu Phe Asp Asn Ser Glu 
                245                 250                 255     


Asp Ala Leu Leu Val Glu Gly Lys Arg Phe Leu Tyr Gln Glu Glu Lys 
            260                 265                 270         


Glu Leu Leu Ala Lys Glu Leu Phe Val Lys Glu Ser Met Lys Lys Ala 
        275                 280                 285             


Glu Val Leu Lys Leu Leu Phe Glu Asn Tyr Gln Glu Leu Asp Leu Asn 
    290                 295                 300                 


Phe Lys Gln Ile Asp Gly Asn His Thr Gly Phe Ala Leu Phe Ser Ala 
305                 310                 315                 320 


Tyr Ser Lys Met Ile Glu Lys Tyr Gly Tyr Glu Pro Val Asp Phe Lys 
                325                 330                 335     


Arg Ser Ala Asp Glu Ile Ile Glu Lys Leu Glu Thr Ile Phe Thr Asn 
            340                 345                 350         


Leu Gly Trp Asn Thr Glu Leu Leu Ser Ile Asp Leu Ser Lys Glu Asp 
        355                 360                 365             


Lys Glu Leu Glu Arg Gln Ser Tyr Phe Arg Leu Trp His Leu Leu Tyr 
    370                 375                 380                 


Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly Asn Gly Lys Leu Leu Glu 
385                 390                 395                 400 


Lys Ile Met Gln Leu Cys Asp Val Glu Lys Glu Tyr Ala Val Glu Leu 
                405                 410                 415     


Ala Asn Val Ser Phe Gln Glu Asp Tyr Gly Ser Leu Ser Ala Lys Ala 
            420                 425                 430         


Ile Lys Lys Ile Leu Pro Tyr Leu Lys Glu Gly Asn Gln Tyr Asp Val 
        435                 440                 445             


Ala Cys Asp Tyr Ala Gly Tyr Arg His Ser Arg Ser Ser Leu Thr Lys 
    450                 455                 460                 


Asp Glu Ile Glu Asn Lys Ile Leu Lys Asp Lys Leu Glu Ile Leu Pro 
465                 470                 475                 480 


Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Met 
                485                 490                 495     


Val Asn Leu Ile Asn Thr Leu Ile Asp Thr Tyr Gly Lys Pro Asp Glu 
            500                 505                 510         


Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Lys Glu Arg 
        515                 520                 525             


Glu Glu Leu Thr Lys Ser Ile Ala Arg Asn Thr Arg Glu His Asp Glu 
    530                 535                 540                 


Ile Arg Gln Leu Leu Arg Thr Glu Phe Gly Met Met Asn Val Ser Arg 
545                 550                 555                 560 


Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Glu Glu Leu Lys Asp Asn Gly 
                565                 570                 575     


Tyr Lys Thr Leu Tyr Ser Asn Glu Tyr Ile Pro Arg Glu Lys Ile Phe 
            580                 585                 590         


Ser Lys Glu Ile Asp Ile Glu His Val Ile Pro Gln Ala Arg Leu Phe 
        595                 600                 605             


Asp Asp Ser Leu Ser Asn Lys Thr Leu Glu Tyr Arg Ala Ile Asn Ile 
    610                 615                 620                 


Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe Val Lys Glu Lys Tyr Gly 
625                 630                 635                 640 


Asn Asp Gly Leu Glu Lys Phe Leu Asn Arg Cys Glu Thr Leu Phe Lys 
                645                 650                 655     


Asp Lys Arg Thr Lys Leu Arg Lys Leu Lys Met Glu Glu Lys Asp Ile 
            660                 665                 670         


Pro Glu Gly Phe Ile Asp Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ser 
        675                 680                 685             


Lys Lys Ala Phe Ala Met Leu Asn Glu Ile Ser Arg Arg Val Val Ala 
    690                 695                 700                 


Thr Thr Gly Ser Ile Thr Asp Lys Leu Arg Glu Asp Trp Gln Leu Val 
705                 710                 715                 720 


Asp Leu Met Lys Glu Leu Asn Leu Pro Lys Tyr Glu Lys Leu Gly Met 
                725                 730                 735     


Val Glu Met Phe Glu Asp Lys Asp Gly Arg Lys Ile Lys Lys Ile Thr 
            740                 745                 750         


Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Val Asp Ala Leu 
        755                 760                 765             


Thr Val Ala Phe Thr Lys Asp Val Phe Ile Gln Phe Tyr Asn Asn Lys 
    770                 775                 780                 


Asn Ala Ala Trp Met Pro Ala Ser Lys Glu His Ala Asn Ile Thr Gly 
785                 790                 795                 800 


Ile Lys Ala Arg Tyr Phe Glu Asn Gly Lys Ala Leu Ala Pro Met Pro 
                805                 810                 815     


Leu Ala Gln Phe Arg Ala Glu Ala Lys Arg His Leu Glu Asn Leu Leu 
            820                 825                 830         


Val Ser Ile Lys Ala Lys Asn Lys Val Ile Thr Ser Asn Val Asn Arg 
        835                 840                 845             


Thr Lys Asn Lys Asn Gly Glu Asn Arg Lys Leu Gln Gln Thr Pro Arg 
    850                 855                 860                 


Gly Gln Leu His Leu Glu Thr Val Tyr Gly Ser His Arg Gln Tyr Ala 
865                 870                 875                 880 


Thr Lys Ile Glu Lys Val Asn Ala Ala Phe Asp Val Thr Lys Ile Ala 
                885                 890                 895     


Thr Val Ser Lys Leu Ala Tyr Arg Asn Ala Leu Leu Lys Arg Leu Glu 
            900                 905                 910         


Ala Phe Gly Asn Asp Pro Lys Lys Ala Phe Thr Gly Lys Asn Thr Leu 
        915                 920                 925             


Glu Lys Asn Pro Leu Tyr Leu Asp Glu Asn Gln Thr Glu Met Val Pro 
    930                 935                 940                 


Glu Lys Val Gln Thr Val Glu Phe Glu Thr Val Tyr Thr Ile Arg Lys 
945                 950                 955                 960 


Pro Ile Asp Pro Asn Leu Ser Ile Asp Lys Val Val Asp Val Lys Ile 
                965                 970                 975     


Arg Ser Val Leu Glu Lys Arg Leu Lys Glu Tyr Gly Gly Asp Ala Lys 
            980                 985                 990         


Lys Ala Phe Val Asn Leu Asp Glu  Asn Pro Ile Trp Leu  Asn Glu Ala 
        995                 1000                 1005             


Lys Gly  Ile Ser Ile Lys Arg  Val Ser Ile Arg Gly  Ile Ser Asn 
    1010                 1015                 1020             


Ala Gln  Ser Leu His Glu Lys  Lys Asp Lys Asp Gly  Asn Leu Ile 
    1025                 1030                 1035             


Leu Gly  Lys Glu Gly Arg Lys  Ile Pro Val Asp Phe  Val Asn Thr 
    1040                 1045                 1050             


Gly Asn  Asn His His Val Ala  Ile Tyr Lys Lys Pro  Val Leu Asp 
    1055                 1060                 1065             


Lys Val  Gly Gln Val Val Leu  Asp Glu Glu Gly Asn  Pro Lys Tyr 
    1070                 1075                 1080             


Glu Leu  Asp Glu Val Val Val  Ser Phe Phe Asp Ala  Val Thr Arg 
    1085                 1090                 1095             


Ala Asn  Leu Gly Gln Pro Ile  Ile Asp Lys Asp Tyr  Arg Arg Ser 
    1100                 1105                 1110             


Glu Gly  Trp Gln Phe Leu Phe  Ser Met Lys Gln Asn  Glu Tyr Phe 
    1115                 1120                 1125             


Val Phe  Pro Asn Asp Lys Thr  Gly Phe Asn Pro Lys  Glu Val Asp 
    1130                 1135                 1140             


Leu Leu  Asn Pro Glu Asn Tyr  Ala Leu Ile Ser Pro  Asn Leu Phe 
    1145                 1150                 1155             


Arg Val  Gln Lys Phe Ala Tyr  Lys Asn Tyr Val Phe  Arg His His 
    1160                 1165                 1170             


Leu Glu  Thr Thr Ile Lys Asp  Thr Ser Ser Thr Leu  Lys Gly Ile 
    1175                 1180                 1185             


Thr Trp  Thr Asp Phe Arg Ser  Ser Lys Gly Leu Asp  Lys Ile Val 
    1190                 1195                 1200             


Lys Val  Arg Val Asp His Ile  Gly Gln Ile Val Ser  Ile Gly Glu 
    1205                 1210                 1215             


Tyr 
    


<210>  779
<211>  1220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  779

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Ser Ala Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Glu Asp Glu Lys Gly Ile Arg Ile Ile Ala Leu Gly 
            20                  25                  30          


Ser Arg Ile Ile Pro Tyr Asn Gly Thr Glu Gly Gln Asp Phe Ala Lys 
        35                  40                  45              


Gly Ala Gly Glu Ser Arg Asn Ser Leu Arg Thr Gln Ala Arg Thr Thr 
    50                  55                  60                  


Arg Lys Gly Tyr Asp Arg Tyr Gln Leu Arg Arg Lys Tyr Leu Ile Asn 
65                  70                  75                  80  


Val Leu Glu Lys Asn Leu Met Met Pro Ser Glu Asp Leu Arg Lys Met 
                85                  90                  95      


Pro Lys Met Asn Leu Trp Gln Leu Arg Ser Lys Ala Val Thr Glu Tyr 
            100                 105                 110         


Ile Ser Arg Gln Glu Leu Gly Arg Leu Leu Leu Trp Leu Asn Gln Lys 
        115                 120                 125             


Arg Gly Tyr Lys Ser Ser Arg Ser Asp Ala Asn Leu Asp Lys Lys Asp 
    130                 135                 140                 


Thr Asp Tyr Val Lys Glu Val Lys Ser Arg Phe Asp Lys Ile Lys Glu 
145                 150                 155                 160 


Lys Asn Gln Thr Ile Gly Gln Tyr Phe Tyr Glu Ser Leu Gln Cys Asp 
                165                 170                 175     


Glu Tyr Phe Arg Val Lys Glu Asn Val Phe Pro Arg Glu Ala Tyr Ile 
            180                 185                 190         


Ala Glu Phe Asp Ala Ile Cys Ser Arg Gln Lys Asp His Leu Gly Leu 
        195                 200                 205             


Ser Glu Glu Leu Ile Ala Glu Ile Arg Asn Glu Ile Ile Tyr Tyr Gln 
    210                 215                 220                 


Arg Pro Leu Lys Ser Gln Lys Gly Leu Val Ser Val Tyr Asp Phe Glu 
225                 230                 235                 240 


Gly Lys Trp Met Asn Gln Asn Gly Lys Glu Leu Phe Ile Gly Pro Lys 
                245                 250                 255     


Val Ala His Lys Ser Ser Pro Leu Phe Gln Leu Ala Lys Ile Trp Glu 
            260                 265                 270         


Ser Val Asn Asn Ile Lys Leu Ser Thr Lys Tyr Gly Asp Thr Ile Glu 
        275                 280                 285             


Leu Thr Leu Glu Glu Lys Lys Gln Leu Val Glu Phe Leu Asp Asn Asn 
    290                 295                 300                 


Glu Lys Leu Thr Thr Ala Ala Leu Phe Lys Ile Leu Arg Lys Asp Lys 
305                 310                 315                 320 


Lys Glu Tyr Ile Leu Pro Lys Gln Leu Glu Lys Gly Leu Gln Gly Asn 
                325                 330                 335     


Ile Thr Lys Cys Lys Ile Ala Asp Ile Leu Gly Ser Lys Tyr Asn Gln 
            340                 345                 350         


Leu Leu Gln Leu Asn Leu Glu Lys Gln Glu Val Gly Thr Pro Cys Tyr 
        355                 360                 365             


Leu Tyr Thr Lys Ser Thr Gly Glu Val Leu Gly Glu Lys Pro Leu Gln 
    370                 375                 380                 


Tyr Ile Asp Lys Ser Val Glu Lys Glu Leu Phe Tyr Gln Leu Trp His 
385                 390                 395                 400 


Thr Ile Tyr Ser Ile Ser Asp Glu Asp Glu Cys Cys Lys Ala Leu Gln 
                405                 410                 415     


Lys Arg Phe Asp Ile Asp Ala Glu Thr Ala Gly Arg Leu Ala Lys Ile 
            420                 425                 430         


Asp Phe Ser Lys Tyr Gly Phe Gly Asn Lys Ser Ala Lys Ala Ile Arg 
        435                 440                 445             


Lys Ile Leu Pro Tyr Leu Met Glu Gly Asp Lys Tyr Ser Asp Ala Met 
    450                 455                 460                 


Cys Tyr Ala Gly Tyr Asn His Ser Asn Ser Leu Thr Lys Glu Glu Asn 
465                 470                 475                 480 


His Glu Arg Lys Leu Asn Asp Lys Leu Lys Leu Leu Thr Lys Asn Ser 
                485                 490                 495     


Leu Arg Gln Pro Ile Val Glu Lys Ile Met Asn Gln Met Ile Gln Leu 
            500                 505                 510         


Val Asn Thr Ile Ile Asp Lys Tyr Gly Lys Pro Asp Glu Ile Arg Leu 
        515                 520                 525             


Glu Leu Ala Arg Glu Leu Lys Gln Ser Lys Glu Glu Arg Asn Asp Ala 
    530                 535                 540                 


Asp Lys Ala Met Ser Lys Arg Gln Arg Glu Asn Lys Glu Ala Glu Lys 
545                 550                 555                 560 


Lys Leu Glu Glu His Gly Ile Arg Ala Thr His Asn Asn Ile Leu Lys 
                565                 570                 575     


Tyr Arg Leu Phe Glu Glu Met Gly Glu Lys Lys His Glu Ala Ile Cys 
            580                 585                 590         


Ile Tyr Cys Gly Asn Pro Ile Ser Phe Ser Ala Ala Met Asn Gly Asp 
        595                 600                 605             


Glu Val Asp Val Glu His Ile Ile Pro Arg Ser Lys Leu Phe Asp Asp 
    610                 615                 620                 


Ser Gln Ser Asn Lys Thr Leu Ala His Arg Lys Cys Asn Ala Asn Lys 
625                 630                 635                 640 


Asn Asp Met Thr Ala Tyr Asp Phe Met Lys Thr Lys Ser Lys Gln Asp 
                645                 650                 655     


Phe Asp Lys Tyr Val Glu Arg Val Asn Gln Leu Tyr Ala Asp Arg Ile 
            660                 665                 670         


Ile Ser Arg Ser Lys Tyr Asn Lys Leu Leu Met Ser Glu Asp Asp Ile 
        675                 680                 685             


Pro Ser Asn Phe Ile Asp Arg Gln Leu Arg Glu Ser Gln Tyr Ile Ala 
    690                 695                 700                 


Thr Lys Ala Arg Glu Ile Leu Gln Thr Ile Cys Tyr Asn Val Trp Ser 
705                 710                 715                 720 


Thr Ser Gly Thr Val Thr Ala Glu Leu Arg His Leu Trp Gly Trp Asp 
                725                 730                 735     


Asp Ile Thr Met Asn Leu Gln Met Pro Lys Tyr Lys Glu Leu Gly Leu 
            740                 745                 750         


Thr Glu Met Val Glu Trp Thr Ser Asp His Gly Lys Asn Val His Ser 
        755                 760                 765             


Lys Glu Val Ile Lys Gly Trp Thr Lys Arg Asp Asp His Arg His His 
    770                 775                 780                 


Ala Ile Asp Ala Leu Thr Ile Ala Cys Thr Lys Gln Gly Phe Ile Gln 
785                 790                 795                 800 


Arg Phe Asn Thr Leu Ser Ser Ser Lys Thr Arg Asp Asp Met Gln Lys 
                805                 810                 815     


Glu Leu Glu Lys Cys Ser Ile Gln Phe Arg Glu Lys Arg Ser Leu Leu 
            820                 825                 830         


Glu Lys Tyr Ile Ile Ser Gln Arg Pro Ile Ser Val Ser Asp Val Ala 
        835                 840                 845             


Glu Ala Val Ser Arg Ile Leu Ile Ser Phe Lys Ala Gly Lys Lys Val 
    850                 855                 860                 


Ala Thr Ile Gly Lys Arg Lys Val Lys Gly Lys Val Val Gln Gln Gly 
865                 870                 875                 880 


Ile Val Val Pro Arg Gly Ala Leu Ser Glu Gly Ser Val Tyr Gly Lys 
                885                 890                 895     


Ile Arg Thr Ile Val Arg Lys Met Pro Val Lys Tyr Leu Phe Glu Asn 
            900                 905                 910         


Pro Asp Leu Ile Phe Lys Pro Tyr Ile Lys Ala Leu Val Lys Glu Arg 
        915                 920                 925             


Leu Ala Glu Phe Asp Asn Asp Asn Lys Gln Ala Leu Ala Ser Leu Lys 
    930                 935                 940                 


Lys Asn Pro Val Tyr Leu Asp Lys Glu Lys Thr Lys Leu Leu Glu Tyr 
945                 950                 955                 960 


Gly Thr Cys Phe Lys Asp Glu Tyr Val Lys Lys Tyr Asp Val Asn Pro 
                965                 970                 975     


Asn Phe Thr Lys Ile Ala Asp Val Ile Asp Gly Lys Val Lys Gln Ile 
            980                 985                 990         


Leu Glu Asp Arg Leu Ser Lys Phe  Gly Gly Lys Pro Lys  Glu Ala Phe 
        995                 1000                 1005             


Lys Asp  Val Val Lys Asp Gly  Lys Thr Leu Lys Trp  Tyr Glu Asp 
    1010                 1015                 1020             


Glu Gly  Leu Glu Arg Pro Ile  Arg Ser Val Arg Cys  Leu Thr Gly 
    1025                 1030                 1035             


Leu Ser  Ala Val Val Pro Val  Lys Lys Asp Glu Asn  Gly Met Asp 
    1040                 1045                 1050             


Ile Gly  Phe Val Lys Pro Gly  Asn Asn His His Val  Ala Ile Tyr 
    1055                 1060                 1065             


Val Gly  Ala Asp Gly Lys Gly  Gln Glu His Val Cys  Thr Phe Trp 
    1070                 1075                 1080             


Asn Ala  Val Glu Arg Lys Lys  Tyr Gly Leu Pro Ile  Ile Ile Lys 
    1085                 1090                 1095             


Asp Ala  Asn Glu Val Trp Asp  Lys Ile Thr Met Ala  Gly Asp Asp 
    1100                 1105                 1110             


Val Tyr  Pro Gln Ser Phe Leu  Ser Leu Leu Pro Lys  Pro Asp Trp 
    1115                 1120                 1125             


Thr Leu  Lys Leu Ser Met Gln  Gln Asn Glu Met Phe  Ile Leu Gly 
    1130                 1135                 1140             


Met Glu  Glu Asp Glu Val Lys  Phe Ala Ile Glu Ser  Lys Asp Tyr 
    1145                 1150                 1155             


Lys Leu  Leu Ser Asp His Leu  Tyr Arg Val Gln Lys  Leu Ser Ser 
    1160                 1165                 1170             


Ser Asp  Tyr Asn Phe Ile His  His Leu Glu Thr Asp  Val Gln Lys 
    1175                 1180                 1185             


Lys Thr  Met Thr Tyr Arg Val  Arg Ser Leu Ser Gly  Leu Phe Ala 
    1190                 1195                 1200             


Leu Arg  Pro Phe Lys Val Arg  Ile Asp Cys Leu Gly  Asn Ile Ile 
    1205                 1210                 1215             


Pro Ser  
    1220 


<210>  780
<211>  1222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  780

Met Lys Lys Arg Ile Leu Gly Leu Asp Ser Gly Thr Asn Ser Leu Gly 
1               5                   10                  15      


Trp Ala Val Val Asp Arg Asp Glu Asn Asn Gln Tyr Ser Leu Ile Lys 
            20                  25                  30          


Tyr Gly Asp Leu Ile Phe Glu Glu Gly Val Lys Ile Glu Lys Gly Val 
        35                  40                  45              


Glu Ser Ser Lys Ala Ala Glu Arg Thr Ser His Arg Ala Leu Arg Lys 
    50                  55                  60                  


Leu Tyr Phe Arg Arg Arg Leu Arg Lys Ile Glu Val Leu Lys Val Leu 
65                  70                  75                  80  


Val Lys Tyr Asn Leu Cys Pro Gln Val Ser Glu Glu Glu Leu His Leu 
                85                  90                  95      


Trp His Thr Arg Lys Leu Tyr Pro Gln Asn Lys Asp Leu Leu Gln Trp 
            100                 105                 110         


Gln Arg Thr Asp Glu Lys Ala Gly Lys Asn Pro Tyr Ile Tyr Arg Tyr 
        115                 120                 125             


Arg Cys Leu His Glu Glu Leu Asp Met Ser Val Ser Ala Asn Arg Met 
    130                 135                 140                 


Thr Val Gly Arg Ala Met Tyr His Leu Ala Gln Arg Arg Gly Phe Leu 
145                 150                 155                 160 


Ser Asn Arg Leu Asp Asn Glu Glu Asp Glu Asn Gln Ile Gly Ala Val 
                165                 170                 175     


Lys Cys Gly Ile Ser Asn Leu Thr Glu Glu Ile Gln Ser Ser Gly Cys 
            180                 185                 190         


Lys Tyr Leu Gly Glu Tyr Phe Tyr Lys Leu Tyr Asn Glu His Gly Asn 
        195                 200                 205             


Thr Val Arg Leu Arg Thr Arg Tyr Thr Ser Arg Glu Phe His Tyr Lys 
    210                 215                 220                 


Lys Glu Phe Tyr Ala Ile Cys Glu Lys Gln Lys Leu Asp Lys Lys Leu 
225                 230                 235                 240 


Ile Glu Glu Leu Ala Ser Ala Leu Tyr Phe Gln Arg Pro Leu Lys Ser 
                245                 250                 255     


Gln Arg His Thr Val Gly Arg Cys Thr Phe Glu Pro Asn Lys Pro Arg 
            260                 265                 270         


Cys Ala Asp Ser His Pro Ala Tyr Glu Glu Phe Arg Met Leu Ser Phe 
        275                 280                 285             


Ile Asn Thr Ile Lys Val Gln Gly Pro Tyr Asp Thr Glu Leu Arg Val 
    290                 295                 300                 


Leu Asn Thr Glu Glu Arg Asp Ser Ile Thr Lys Leu Phe Cys Arg Lys 
305                 310                 315                 320 


Ser Lys Pro Asn Phe Asp Phe Glu Asp Ile Ala Lys Glu Leu Ala Gly 
                325                 330                 335     


Arg Asn Asn Tyr Cys Tyr Ile Gly Asp Val Val Asp Lys Ala Tyr Lys 
            340                 345                 350         


Phe Asn Tyr Arg Met Ser Gln Gly Val Ser Gly Cys Pro Thr Thr Thr 
        355                 360                 365             


Tyr Leu Ile Asn Val Phe Gly Gln Asp Trp Gln Thr Gly Ile Ala Glu 
    370                 375                 380                 


Ser Tyr Gln Ala Ile Asp Thr Lys Leu Gly Gln Lys Ser Val Asp Ala 
385                 390                 395                 400 


Met Ile Asp Asp Val Trp Asn Val Leu Tyr Ser Phe Ser Ser Lys Asp 
                405                 410                 415     


Lys Leu Arg Asp Phe Gly Arg Lys Lys Leu Gln Leu Gly Asp Lys Glu 
            420                 425                 430         


Ala Asp Thr Phe Ala Asn Ile Lys Leu Thr His Lys Phe Ala Ser Leu 
        435                 440                 445             


Ser Leu Lys Ala Ile Arg Lys Ile Asn Ile Phe Leu Arg Met Gly Leu 
    450                 455                 460                 


Val Tyr Ser His Ala Val Thr Leu Ala Asn Ile Pro Thr Ile Val Gly 
465                 470                 475                 480 


Thr Asp Ile Trp Gln Arg Lys Arg Asn Phe Ile Leu Ala Glu Leu Leu 
                485                 490                 495     


Pro Leu Ile Glu Asn Phe Asn Pro Lys Glu Thr Lys Leu Gln Glu Thr 
            500                 505                 510         


Leu Asp Phe Cys Val Lys Asp Phe Leu Gln Asn Asn Phe Glu Leu Lys 
        515                 520                 525             


Asn Gly Ala Val Asp Lys Leu Tyr His Pro Ser Met Ile Glu Ala Tyr 
    530                 535                 540                 


Pro Asp Ala Gly Leu Asn Ser Asn Gly Ile Leu Gln Leu Gly Ser Pro 
545                 550                 555                 560 


Arg Thr Asn Ala Ile Arg Asn Pro Met Ala Met Arg Ser Leu His Glu 
                565                 570                 575     


Ile Arg Lys Val Ile Asn Gln Leu Leu Lys Asp Lys Ile Ile Asn Gln 
            580                 585                 590         


Asp Thr Glu Ile His Val Glu Tyr Ala Arg Glu Leu Asn Asp Ala Asn 
        595                 600                 605             


Lys Arg Lys Ala Ile Ser Ile Trp Gln Lys Ser Gln Glu Lys Leu His 
    610                 615                 620                 


Lys Lys Tyr Val Glu Glu Ile Thr Asn Leu Tyr Lys Glu Glu Thr Gly 
625                 630                 635                 640 


Lys Ala Ile Val Pro Thr Asp Thr Asp Ile Leu Lys Phe Lys Leu Trp 
                645                 650                 655     


Glu Glu Gln Asn His Ile Cys Leu Tyr Thr Gly Gln Gln Ile Gly Ile 
            660                 665                 670         


Ala Glu Phe Leu Gly Ala Thr Pro Lys Tyr Asp Ile Glu His Thr Ile 
        675                 680                 685             


Pro Gln Ser Val Gly Gly Asp Ser Thr Asp Met Asn Leu Thr Leu Cys 
    690                 695                 700                 


Asp Ser Arg Phe Asn Arg Glu Ile Lys Lys Ala Gln Leu Pro Thr Gln 
705                 710                 715                 720 


Leu Gly Asn His Ala Asp Ile Leu Val Arg Ile Ala Gly Trp Lys Asn 
                725                 730                 735     


Ser Tyr Glu Lys Leu Ser Lys Asp Ile Asp Arg Ile Arg Thr His Ser 
            740                 745                 750         


Gly Met Ala Lys Asp Ile Lys Asp Arg Leu Ile Gln Lys Arg His Leu 
        755                 760                 765             


Leu Lys Leu Glu Arg Asp Tyr Trp Arg Gly Lys Tyr Glu Arg Phe Thr 
    770                 775                 780                 


Met Asn Glu Ile Pro Glu Gly Phe Ser Arg Arg Gln Gly Val Gly Ile 
785                 790                 795                 800 


Gly Leu Ile Ser Lys Tyr Ala Gly Leu Tyr Leu Lys Ser Leu Phe His 
                805                 810                 815     


Asp Lys Asn Glu Arg Asn Lys Gly His Val Phe Val Val Lys Gly Thr 
            820                 825                 830         


Thr Thr Ala Glu Phe Arg Lys Met Trp Gly Leu Gln Asn Glu Tyr Glu 
        835                 840                 845             


Lys Lys Ser Arg Asn Asn His Val His His Cys Ile Asp Ala Ile Thr 
    850                 855                 860                 


Ile Ala Cys Ile Gly Lys His Glu Tyr Ala Gln Met Ala Gln Tyr Tyr 
865                 870                 875                 880 


His Asp Glu Ala Glu Tyr Leu Tyr His His Gly Ile Lys Pro Ile Phe 
                885                 890                 895     


Pro Lys Pro Trp Lys Thr Phe Thr Glu Asp Leu Leu Asp Leu Glu His 
            900                 905                 910         


Ser Leu Phe Val Val His His Ser Pro Asn Asn Met Ala Lys Gln Ala 
        915                 920                 925             


Arg Lys Arg Ile Ile Thr Ser Thr Gly Gln His Trp Ala Gln Gly Asp 
    930                 935                 940                 


Ser Ala His Gly Ser Leu His Asn Asp Thr Tyr Tyr Gly Ala Ile Glu 
945                 950                 955                 960 


Arg Asp Gly Glu Ile Lys Tyr Val Val Arg Arg Ala Val Ser Ser Phe 
                965                 970                 975     


Glu Gln Met Ser Asp Val Asp Ser Ile Val Asp Asp Val Val Lys Glu 
            980                 985                 990         


Thr Val Arg Lys Ala Leu Glu Gly  Lys Asn Phe Lys Glu  Ala Val Asn 
        995                 1000                 1005             


Ala Pro  Ile Tyr Met Asn Lys  Glu Lys Gly Val Leu  Ile Lys Lys 
    1010                 1015                 1020             


Val Arg  Cys Tyr Ala Lys Ser  Ile Lys Lys Pro Ile  Asp Ile Arg 
    1025                 1030                 1035             


Thr His  Arg Asp Ser Ser Ser  Lys Glu Tyr Lys Arg  Lys Phe His 
    1040                 1045                 1050             


Val Ser  Asn Asp Ile Asn Tyr  Leu Ile Ala Ile Tyr  Glu Gly Thr 
    1055                 1060                 1065             


Val Lys  Gly Lys Val Lys Arg  Asp Phe Glu Val Val  Lys Asn Ile 
    1070                 1075                 1080             


Glu Ala  Ala Asn Phe Phe Lys  Arg Ser Gln Asp Arg  Arg Asp Tyr 
    1085                 1090                 1095             


Pro Thr  Ile Val Pro Glu Lys  Ser Ile Lys His Gly  Phe Pro Leu 
    1100                 1105                 1110             


Lys Thr  Thr Leu Thr Asn Gly  Thr Met Val Ile Leu  Tyr Glu Glu 
    1115                 1120                 1125             


Asn Lys  Glu Glu Ile Asp Phe  Thr Lys Lys Glu Asp  Ile Cys Arg 
    1130                 1135                 1140             


Arg Leu  Tyr Lys Leu Val Gly  Met Ser Ser Met Ile  Val Ser Gly 
    1145                 1150                 1155             


Cys Glu  Tyr Gly Val Leu Ser  Leu Arg His His Gln  Glu Ala Arg 
    1160                 1165                 1170             


Pro Ser  Lys Asp Ile Lys Ser  Lys Asn Gly Ala Phe  Lys Asn Gly 
    1175                 1180                 1185             


Glu Ala  Leu Arg Pro Ala Ile  Thr Met Leu His Thr  Gln Phe Asn 
    1190                 1195                 1200             


Ala Leu  Val Glu Asn Ile Asp  Phe Asn Ile Asp Val  Thr Gly Asn 
    1205                 1210                 1215             


Ile Glu  Leu Ile 
    1220         


<210>  781
<211>  1222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  781

Met Lys Lys Arg Ile Leu Gly Leu Asp Ser Gly Thr Asn Ser Leu Gly 
1               5                   10                  15      


Trp Ala Val Val Asp Arg Asp Glu Asn Asn Gln Tyr Ser Leu Ile Lys 
            20                  25                  30          


Tyr Gly Asp Leu Ile Phe Glu Glu Gly Val Lys Ile Glu Lys Gly Val 
        35                  40                  45              


Glu Ser Ser Lys Ala Ala Glu Arg Thr Ser His Arg Ala Leu Arg Lys 
    50                  55                  60                  


Leu Tyr Phe Arg Arg Arg Leu Arg Lys Ile Glu Val Leu Lys Val Leu 
65                  70                  75                  80  


Val Lys Tyr Asn Leu Cys Pro Gln Val Ser Glu Glu Glu Leu His Leu 
                85                  90                  95      


Trp His Thr Arg Lys Leu Tyr Pro Gln Asn Lys Asp Leu Leu Gln Trp 
            100                 105                 110         


Gln Arg Thr Asp Glu Lys Glu Gly Lys Asn Pro Tyr Ile Tyr Arg Tyr 
        115                 120                 125             


Arg Cys Leu His Glu Glu Leu Asp Met Ser Val Ser Ala Asn Arg Met 
    130                 135                 140                 


Thr Val Gly Arg Ala Met Tyr His Leu Ala Gln Arg Arg Gly Phe Leu 
145                 150                 155                 160 


Ser Asn Arg Leu Asp Asn Glu Glu Asp Glu Asn Gln Ile Gly Ala Val 
                165                 170                 175     


Lys Cys Gly Ile Ser Asn Leu Thr Glu Glu Ile Gln Ser Ser Gly Cys 
            180                 185                 190         


Lys Tyr Leu Gly Glu Tyr Phe Tyr Lys Leu Tyr Asn Glu His Gly Asn 
        195                 200                 205             


Thr Val Arg Leu Arg Thr Arg Tyr Thr Ser Arg Glu Phe His Tyr Lys 
    210                 215                 220                 


Lys Glu Phe Tyr Ala Ile Cys Glu Lys Gln Lys Leu Asp Lys Lys Leu 
225                 230                 235                 240 


Ile Glu Glu Leu Ala Ser Ala Leu Tyr Phe Gln Arg Pro Leu Lys Ser 
                245                 250                 255     


Gln Arg His Thr Val Gly Arg Cys Thr Phe Glu Pro Asn Lys Pro Arg 
            260                 265                 270         


Cys Ala Asp Ser His Pro Ala Tyr Glu Glu Phe Arg Met Leu Ser Phe 
        275                 280                 285             


Ile Asn Thr Ile Lys Val Gln Gly Pro Tyr Asp Thr Glu Leu Arg Val 
    290                 295                 300                 


Leu Asn Thr Glu Glu Arg Asp Ser Ile Thr Lys Leu Phe Cys Arg Lys 
305                 310                 315                 320 


Ser Lys Pro Asn Phe Asp Phe Glu Asp Ile Ala Lys Glu Leu Ala Gly 
                325                 330                 335     


Arg Asn Asn Tyr Cys Tyr Ile Gly Asp Val Val Asp Lys Ala Tyr Lys 
            340                 345                 350         


Phe Asn Tyr Arg Met Ser Gln Gly Val Ser Gly Cys Pro Thr Thr Thr 
        355                 360                 365             


Tyr Leu Ile Asn Val Phe Gly Gln Asp Trp Gln Thr Gly Ile Ala Glu 
    370                 375                 380                 


Ser Tyr Gln Ala Ile Asp Thr Lys Leu Gly Gln Lys Ser Val Asp Ala 
385                 390                 395                 400 


Met Ile Asp Asp Val Trp Asn Val Leu Tyr Ser Phe Ser Ser Lys Asp 
                405                 410                 415     


Lys Leu Arg Asp Phe Gly Arg Lys Lys Leu Gln Leu Gly Asp Lys Glu 
            420                 425                 430         


Ala Asp Thr Phe Ala Asn Ile Lys Leu Thr His Lys Phe Ala Ser Leu 
        435                 440                 445             


Ser Leu Lys Ala Ile Arg Lys Ile Asn Ile Phe Leu Arg Met Gly Leu 
    450                 455                 460                 


Val Tyr Ser His Ala Val Thr Leu Ala Asn Ile Pro Thr Ile Val Gly 
465                 470                 475                 480 


Thr Asp Ile Trp Gln Arg Lys Arg Asn Phe Ile Leu Ala Glu Leu Leu 
                485                 490                 495     


Pro Leu Ile Glu Asn Phe Asn Pro Lys Glu Thr Lys Leu Gln Glu Thr 
            500                 505                 510         


Leu Asp Phe Cys Val Lys Asp Phe Leu Gln Asn Asn Phe Glu Leu Lys 
        515                 520                 525             


Asn Gly Ala Val Asp Lys Leu Tyr His Pro Ser Met Ile Glu Ala Tyr 
    530                 535                 540                 


Pro Asp Ala Gly Leu Asn Ser Asn Gly Ile Leu Gln Leu Gly Ser Pro 
545                 550                 555                 560 


Arg Thr Asn Ala Ile Arg Asn Pro Met Ala Met Arg Ser Leu His Glu 
                565                 570                 575     


Ile Arg Lys Val Ile Asn Gln Leu Leu Lys Asp Lys Ile Ile Asn Gln 
            580                 585                 590         


Asp Thr Glu Ile His Val Glu Tyr Ala Arg Glu Leu Asn Asp Ala Asn 
        595                 600                 605             


Lys Arg Lys Ala Ile Ser Ile Trp Gln Lys Ser Gln Glu Lys Leu His 
    610                 615                 620                 


Lys Lys Tyr Val Glu Glu Ile Thr Asn Leu Tyr Lys Glu Glu Thr Gly 
625                 630                 635                 640 


Lys Ala Ile Val Pro Thr Asp Thr Asp Ile Leu Lys Phe Lys Leu Trp 
                645                 650                 655     


Glu Glu Gln Asn His Ile Cys Leu Tyr Thr Gly Gln Gln Ile Gly Ile 
            660                 665                 670         


Ala Glu Phe Leu Gly Ala Thr Pro Lys Tyr Asp Ile Glu His Thr Ile 
        675                 680                 685             


Pro Gln Ser Val Gly Gly Asp Ser Thr Asp Met Asn Leu Thr Leu Cys 
    690                 695                 700                 


Asp Ser Arg Phe Asn Arg Glu Ile Lys Lys Ala Gln Leu Pro Thr Gln 
705                 710                 715                 720 


Leu Gly Asn His Ala Asp Ile Leu Val Arg Ile Ala Gly Trp Lys Asn 
                725                 730                 735     


Ser Tyr Glu Lys Leu Ser Lys Asp Ile Asp Arg Ile Arg Thr His Ser 
            740                 745                 750         


Gly Met Ala Lys Asp Ile Lys Asp Arg Leu Ile Gln Lys Arg His Leu 
        755                 760                 765             


Leu Lys Leu Glu Arg Asp Tyr Trp Arg Gly Lys Tyr Glu Arg Phe Thr 
    770                 775                 780                 


Met Asn Glu Ile Pro Glu Gly Phe Ser Arg Arg Gln Gly Val Gly Ile 
785                 790                 795                 800 


Gly Leu Ile Ser Lys Tyr Ala Gly Leu Tyr Leu Lys Ser Leu Phe His 
                805                 810                 815     


Asp Lys Asn Glu Arg Asn Lys Gly His Val Phe Val Val Lys Gly Thr 
            820                 825                 830         


Thr Thr Ala Glu Phe Arg Lys Met Trp Gly Leu Gln Asn Glu Tyr Glu 
        835                 840                 845             


Lys Lys Ser Arg Asn Asn His Val His His Cys Ile Asp Ala Ile Thr 
    850                 855                 860                 


Ile Ala Cys Ile Gly Lys His Glu Tyr Ala Gln Met Ala Gln Tyr Tyr 
865                 870                 875                 880 


His Asp Glu Ala Glu Tyr Leu Tyr His His Gly Ile Lys Pro Ile Phe 
                885                 890                 895     


Pro Lys Pro Trp Lys Thr Phe Thr Glu Asp Leu Leu Asp Leu Glu His 
            900                 905                 910         


Ser Leu Phe Val Val His His Ser Pro Asn Asn Met Ala Lys Gln Ala 
        915                 920                 925             


Arg Lys Arg Ile Ile Thr Ser Thr Gly Gln His Trp Ala Gln Gly Asp 
    930                 935                 940                 


Ser Ala His Gly Ser Leu His Asn Asp Thr Tyr Tyr Gly Ala Ile Glu 
945                 950                 955                 960 


Arg Asp Gly Glu Ile Lys Tyr Val Val Arg Arg Ala Val Ser Ser Phe 
                965                 970                 975     


Glu Gln Met Ser Asp Val Asp Ser Ile Val Asp Asp Val Val Lys Glu 
            980                 985                 990         


Thr Val Arg Lys Ala Leu Glu Gly  Lys Asn Phe Lys Glu  Ala Val Asn 
        995                 1000                 1005             


Ala Pro  Ile Tyr Met Asn Lys  Glu Lys Gly Val Leu  Ile Lys Lys 
    1010                 1015                 1020             


Val Arg  Cys Tyr Ala Lys Ser  Ile Lys Lys Pro Ile  Asp Ile Arg 
    1025                 1030                 1035             


Thr His  Arg Asp Ser Ser Ser  Lys Glu Tyr Lys Arg  Lys Phe His 
    1040                 1045                 1050             


Val Ser  Asn Asp Ile Asn Tyr  Leu Ile Ala Ile Tyr  Glu Gly Thr 
    1055                 1060                 1065             


Val Lys  Gly Lys Val Lys Arg  Asp Phe Glu Val Val  Lys Asn Ile 
    1070                 1075                 1080             


Glu Ala  Ala Asn Phe Phe Lys  Arg Ser Gln Asp Arg  Arg Asp Tyr 
    1085                 1090                 1095             


Pro Thr  Ile Val Pro Glu Lys  Ser Ile Lys His Gly  Phe Pro Leu 
    1100                 1105                 1110             


Lys Thr  Thr Leu Thr Asn Gly  Thr Met Val Ile Leu  Tyr Glu Glu 
    1115                 1120                 1125             


Asn Lys  Glu Glu Ile Asp Phe  Thr Lys Lys Glu Asp  Ile Cys Arg 
    1130                 1135                 1140             


Arg Leu  Tyr Lys Leu Val Gly  Met Ser Ser Met Ile  Val Ser Gly 
    1145                 1150                 1155             


Cys Glu  Tyr Gly Val Leu Ser  Leu Arg His His Gln  Glu Ala Arg 
    1160                 1165                 1170             


Pro Ser  Lys Asp Ile Lys Ser  Lys Asn Gly Ala Phe  Lys Asn Gly 
    1175                 1180                 1185             


Glu Ala  Leu Arg Pro Ala Ile  Thr Met Leu His Thr  Gln Phe Asn 
    1190                 1195                 1200             


Ala Leu  Val Glu Asn Ile Asp  Phe Asn Ile Asp Val  Thr Gly Asn 
    1205                 1210                 1215             


Ile Glu  Leu Ile 
    1220         


<210>  782
<211>  1225
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  782

Met Ser Lys Arg Ile Leu Gly Ile Asp Val Gly Thr Asn Ser Leu Gly 
1               5                   10                  15      


Trp Ala Val Val Asp Arg Thr Asp Cys Asp Asn Tyr Thr Leu Val Lys 
            20                  25                  30          


Arg Gly Asp Val Ile Phe Gln Glu Gly Val Asn Ile Glu Lys Gly Asn 
        35                  40                  45              


Ile Glu Lys Ser Arg Ala Ser Glu Arg Thr Gly His Arg Ser Thr Arg 
    50                  55                  60                  


Lys His Tyr Phe Arg Arg Arg Met Leu Lys Ile Lys Val Leu Glu Ile 
65                  70                  75                  80  


Leu Val Glu His Gln Leu Cys Pro Tyr Leu Ser His Glu Glu Leu His 
                85                  90                  95      


Gln Trp Met Thr Arg Lys Val Tyr Pro Thr Asn Glu Asp Phe Arg Leu 
            100                 105                 110         


Trp Leu Arg Thr Asn Asp Ala Asn Asp Gln Asn Pro Tyr His Asp Arg 
        115                 120                 125             


His Val Cys Leu His Glu Arg Leu Asp Leu Arg Leu Gln Glu Asn Arg 
    130                 135                 140                 


Tyr Arg Leu Gly Arg Ala Leu Tyr His Leu Ala Gln Arg Arg Gly Phe 
145                 150                 155                 160 


Leu Ser Asn Arg Leu Glu Gln Asn Ser Lys Thr Asp Glu Thr Gly Ala 
                165                 170                 175     


Val Lys Ser Asp Ile Ser Gln Leu Asn Glu Glu Met Lys Lys Ala Gly 
            180                 185                 190         


Ser Gln Tyr Leu Gly Asp Tyr Phe Tyr Trp Leu Tyr Gln Glu Tyr Gly 
        195                 200                 205             


Asn Gln Val Thr Leu Arg Ile Arg Tyr Thr Asp Arg Lys Gln His Tyr 
    210                 215                 220                 


Gln Lys Glu Phe Glu Ala Ile Cys Gln Arg Gln Gln Leu Ser Asp Asp 
225                 230                 235                 240 


Leu Val Asp Lys Leu Arg Arg Ala Ile Tyr Phe Gln Arg Ser Leu Lys 
                245                 250                 255     


Ser Gln Arg Ala Gly Val Gly Lys Cys Thr Phe Glu Pro Asp Lys Pro 
            260                 265                 270         


Arg Cys Ala Leu Ser His Pro Arg Tyr Glu Glu Phe Arg Met Leu Ser 
        275                 280                 285             


Phe Leu Asn Asn Val Lys Val Lys Gly Pro Ser Asp Asp Ser Leu Arg 
    290                 295                 300                 


Pro Leu Ser Pro Ser Glu Lys Glu Lys Ile Lys Pro Leu Phe Leu Arg 
305                 310                 315                 320 


Lys Ser Lys Ala Asn Phe Asp Phe Glu Asp Ile Ala Lys Ala Ile Ala 
                325                 330                 335     


Gly Lys Gly Lys Tyr Ala Cys Ile Arg Asp Lys Glu Gly Ala Gln Lys 
            340                 345                 350         


Pro Tyr Gln Phe Asn Tyr Ala Leu Ser Gln Gly Val Ser Gly Cys Pro 
        355                 360                 365             


Val Thr Ala Lys Leu Ile Ala Val Phe Gly Glu Ser Trp Glu Arg Asn 
    370                 375                 380                 


Leu Val Asn Ala Tyr Arg Ser Arg Asn Asp Met Arg Lys Ser Asp Glu 
385                 390                 395                 400 


Glu Ile Val Asn Glu Val Trp Asn Val Leu Tyr Ser Phe Ser Ser Lys 
                405                 410                 415     


Glu Lys Leu Arg Ala Trp Gly Met Glu Lys Leu Gly Leu Asp Glu Ala 
            420                 425                 430         


Cys Ala Gly Glu Phe Ala Asp Ile Thr Leu Pro Thr Ala Phe Ala Ala 
        435                 440                 445             


Leu Ser Leu Lys Ala Val Asn Lys Ile Leu Pro Phe Leu Arg Gln Gly 
    450                 455                 460                 


Phe Ile Tyr Ser His Ala Val Met Leu Ala Asn Ile Pro Tyr Ile Ile 
465                 470                 475                 480 


Gly Met Asp Arg Trp Asn Glu Pro Arg Gln Arg Gln Phe Ile Met Asp 
                485                 490                 495     


Asn Leu Gln Glu Leu Leu Asp Asn Phe Thr Ala Ser Asp Lys Ala Leu 
            500                 505                 510         


Gln Gln Thr Leu Asp Phe Cys Ile Lys Asp Phe Leu Leu Asn Asn Tyr 
        515                 520                 525             


Glu Leu Ala Pro Gly Lys Ala Asp Arg Leu Tyr His Pro Ser Met Ile 
    530                 535                 540                 


Glu Thr Tyr Pro Asp Ala Lys Ala Asp Gly Gln Gly Ile Tyr Gln Leu 
545                 550                 555                 560 


Gly Ser Pro Ala Thr Asn Ala Ile Arg Asn Pro Met Ala Met Arg Ser 
                565                 570                 575     


Leu His Ile Leu Arg Arg Val Val Asn Gln Leu Leu Lys Glu Lys Thr 
            580                 585                 590         


Ile Asp Ser Glu Thr Glu Val His Ile Glu Tyr Ala Arg Glu Leu Asn 
        595                 600                 605             


Asp Ala Asn Arg Arg Trp Ala Ile Asn Gln Trp Gln Arg Lys Gln Glu 
    610                 615                 620                 


Thr Gln Arg Lys Asp Tyr Ala Glu Lys Ile Arg Lys Val Tyr Ala Asp 
625                 630                 635                 640 


Glu Phe Glu Lys Val Ile Glu Pro Thr Gln Glu Glu Leu Leu Lys Tyr 
                645                 650                 655     


Gln Leu Trp Glu Glu Gln Gln His Leu Cys Leu Tyr Thr Gly Glu Gln 
            660                 665                 670         


Ile Gly Leu Thr Asp Phe Ile Gly Pro Asn Pro Arg Tyr Asp Ile Glu 
        675                 680                 685             


His Thr Ile Pro Gln Ser Met Gly Gly Asp Ser Thr Arg Met Asn Leu 
    690                 695                 700                 


Thr Leu Cys Glu Arg Thr Tyr Asn Arg Thr Val Lys Gly Asn Arg Met 
705                 710                 715                 720 


Pro Ser Gln Leu Ala Glu His Glu Ser Ile Leu Thr Arg Ile Lys Pro 
                725                 730                 735     


Trp Lys Asp Lys Tyr Glu Glu Leu Thr Val Lys Ile Asn Lys Leu Arg 
            740                 745                 750         


Thr His Pro Gly Met Asp Lys Ala Ser Lys Asp Asn Ile Ile Arg Lys 
        755                 760                 765             


Arg His Leu Leu Glu Leu Glu Arg Asp Tyr Trp Phe Gln Lys Tyr Gln 
    770                 775                 780                 


Arg Phe Thr Met Thr Glu Val Pro Glu Gly Phe Ser Leu Arg Gln Gly 
785                 790                 795                 800 


Thr Gly Ile Gly Leu Ile Gly Lys Tyr Ala Gly Leu Phe Leu Lys Ser 
                805                 810                 815     


Leu Phe His His Val Lys Glu Arg Asn Arg Lys Gln Val Tyr Val Ile 
            820                 825                 830         


Lys Gly Pro Ile Thr Ala Glu Phe Arg Arg Leu Trp Gly Leu Gln Asn 
        835                 840                 845             


Leu Tyr Glu Gln Lys Ser Arg Asp Asn His Ala His His Cys Ile Asp 
    850                 855                 860                 


Ala Ile Val Ile Ala Cys Ile Asn Pro Ala Glu Tyr Ala Arg Met Ala 
865                 870                 875                 880 


Ser Tyr Tyr Arg Gln Glu Glu Asp Phe Arg Leu Asn Lys Gly Asn Lys 
                885                 890                 895     


Pro Val Phe Pro Lys Pro Trp Thr Thr Phe Thr Gln Asp Leu Gln Ala 
            900                 905                 910         


Leu Glu Ala Glu Leu Leu Val Val His Glu Thr Arg Asn Asn Leu Thr 
        915                 920                 925             


Lys Lys Ala Arg Lys Arg Val Arg Thr Lys Gly Gly Asn Val Leu Ser 
    930                 935                 940                 


Glu Ser Asp Ser Ala Arg Gly Ser Leu His Asn Asp Thr Tyr Tyr Gly 
945                 950                 955                 960 


Ala Ile Gln Lys Asp Gly Val Ile Arg Tyr Val Val Arg Lys Ala Leu 
                965                 970                 975     


Ser Ser Leu Ser Glu Lys Asp Val Lys Asp Ile Val Asp Lys Thr Val 
            980                 985                 990         


Arg Glu Lys Val Gln Ala Ala Ile  Gln Glu Lys Gly Ala  Lys Glu Ala 
        995                 1000                 1005             


Leu Ala  Gly Thr Ile Tyr Met  Asn Glu Glu Lys Gly  Ile Thr Ile 
    1010                 1015                 1020             


Lys Lys  Val Arg Cys Phe Thr  Lys Ile Ala Ser Pro  Leu Ala Ile 
    1025                 1030                 1035             


Arg Arg  His Arg Asp Ala Ser  Ala Lys Glu Tyr Lys  Arg Pro Tyr 
    1040                 1045                 1050             


Tyr Val  Thr Asn Glu Ser Asn  Tyr Val Met Gly Ile  Tyr Glu Gly 
    1055                 1060                 1065             


Cys Ile  Lys Glu Lys Thr Lys  Arg Glu Phe Ile Leu  Val Asn Asn 
    1070                 1075                 1080             


Leu Glu  Ala Val Arg Gln Leu  Lys Ala Ala Arg Lys  Gln Gly Val 
    1085                 1090                 1095             


Val Ser  Ser Ile Leu Pro Pro  Val Ser Ala Lys Ser  Gln Phe Pro 
    1100                 1105                 1110             


Leu Lys  Tyr Arg Leu Tyr Thr  Gly Thr Leu Val Leu  Leu Tyr Glu 
    1115                 1120                 1125             


Asn Ser  Pro Glu Glu Ile Asp  Phe Thr Asn Gln Val  Asp Ile Cys 
    1130                 1135                 1140             


Lys Arg  Leu Tyr Lys Val Thr  Gly Leu Ser Ser Met  Thr Val Thr 
    1145                 1150                 1155             


Gly Asn  Gln Tyr Gly Thr Ile  Gln Leu Arg Tyr His  Gln Glu Ala 
    1160                 1165                 1170             


Arg Gln  Ala Lys Asp Leu Lys  Ala Gln Asn Gly Ala  Tyr Lys Glu 
    1175                 1180                 1185             


Gly Glu  Glu His Arg Pro Ala  Ile Met Met Leu His  Thr Gln Phe 
    1190                 1195                 1200             


Lys Ala  Leu Val Glu Gly Thr  Asp Phe Arg Met Asn  Ile Leu Gly 
    1205                 1210                 1215             


Glu Ile  Glu Pro Leu Arg Lys  
    1220                 1225 


<210>  783
<211>  1229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  783

Met Gly Asp Arg Lys Tyr Asn Leu Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Phe Ala Ala Val Asp Glu Asn Asn Gln Pro Ile Arg Val Lys 
            20                  25                  30          


Gly Lys Thr Ala Ile Gly Val Arg Leu Phe Glu Glu Gly Lys Thr Ala 
        35                  40                  45              


Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Arg Trp Arg Ile Asn Leu Leu Asn Glu Ile Phe Asp Ala His Leu Ala 
65                  70                  75                  80  


Glu Val Asp Pro Thr Phe Leu Ala Arg Leu Lys Glu Ser Asn Arg Ser 
                85                  90                  95      


Asn Leu Asp Pro Lys Lys Ser Phe Gln Gly Ser Leu Leu Phe Pro Glu 
            100                 105                 110         


Arg Lys Asp Tyr Gln Phe Tyr Glu Glu Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Lys Ala Leu Met Glu Lys Asp Arg Lys Phe Asp Ile Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Val His His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Asn 
145                 150                 155                 160 


Gly Thr Pro Met Arg Ser Phe Lys Val Glu Asn Ile Glu Leu Asn Thr 
                165                 170                 175     


Leu Phe Asp Gln Leu Asn Gln Leu Tyr Ala Glu Ile Val Pro Asp Asn 
            180                 185                 190         


Glu Leu Ala Phe Asp Leu Ala Gln Val Ala Asp Val Lys Asp Val Leu 
        195                 200                 205             


Ser Ser Thr Thr Ile Tyr Lys Met Asp Lys Lys Lys Gln Leu Val Lys 
    210                 215                 220                 


Met Met Leu Leu Pro Ala Ser Asn Lys Ala Leu Gln Ser Glu Asn Lys 
225                 230                 235                 240 


Lys Ile Val Thr Gln Phe Val Asn Ala Ile Leu Asn Tyr Lys Phe Lys 
                245                 250                 255     


Leu Asp Val Leu Leu Gln Val Glu Thr Asp Ala Asp Trp Ser Leu Lys 
            260                 265                 270         


Leu Asn Asp Glu Gly Ala Asp Asp Lys Leu Glu Glu Phe Thr Gly Asp 
        275                 280                 285             


Leu Asp Glu Asn Arg Leu Glu Ile Ile Asp Leu Leu Gln Arg Leu His 
    290                 295                 300                 


Asn Trp Phe Ser Leu Asn Glu Ile Thr Lys Asp Gly Asn Ser Leu Ser 
305                 310                 315                 320 


Ala Ala Met Val Glu Lys Tyr Glu Asn His His His His Leu Gly Leu 
                325                 330                 335     


Leu Lys Lys Val Ile Glu Asn His Pro Asp Ala Lys Lys Ala Lys Ala 
            340                 345                 350         


Leu Lys Glu Thr Tyr Thr Ala Tyr Val Gly Lys Thr Asp Asp Lys Thr 
        355                 360                 365             


Gln Asn Gln Asp Asp Phe Tyr Lys Ala Val Glu Lys Asn Leu Asp Asp 
    370                 375                 380                 


Ser Pro Asp Ala Lys Gly Ile Lys Arg Leu Ile Gln Leu Asp Gln Phe 
385                 390                 395                 400 


Met Pro Lys Gln Arg Thr Gly Gln Asn Gly Ala Ile Pro His Gln Leu 
                405                 410                 415     


His Gln Gln Glu Leu Asp Gln Ile Ile Glu Lys Gln Ser Lys Tyr Tyr 
            420                 425                 430         


Pro Phe Leu Ala Glu Pro Asn Pro Asn Val Lys Arg Arg Lys Asp Ala 
        435                 440                 445             


Pro Tyr Lys Leu Asp Glu Leu Ile Ala Phe Lys Ile Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Leu Val Thr Pro Glu Glu Gln Ala Gln Asn Lys Glu Asn Val 
465                 470                 475                 480 


Phe Ala Trp Met Lys Arg Lys Ala Ala Gly Pro Ile Thr Pro Trp Asn 
                485                 490                 495     


Phe Asp Glu Lys Val Asp Arg Met Glu Ser Ala Asn Arg Phe Ile Arg 
            500                 505                 510         


Arg Met Thr Thr Lys Asp Thr Tyr Leu Phe Gly Glu Asp Val Leu Pro 
        515                 520                 525             


Ala Glu Ser Met Ile Tyr Gln Lys Phe Val Val Leu Asn Glu Leu Asn 
    530                 535                 540                 


Asn Leu Lys Ile Asn Gly Arg His Leu Ser Leu Lys Asp Lys Gln Asp 
545                 550                 555                 560 


Val Tyr Asn Asp Leu Phe Lys Gln Gln Lys Thr Val Ser Ile Lys Ala 
                565                 570                 575     


Leu Gln Asn Tyr Tyr Val Thr Lys Lys Lys Ala Ala Thr Ala Pro Thr 
            580                 585                 590         


Val Gly Gly Leu Ala Asp Pro Lys Lys Phe Leu Ser Ser Leu Ser Thr 
        595                 600                 605             


Tyr Ile Asp Phe Lys Asn Met Phe Gly Glu Arg Val Asn Asp Pro Gln 
    610                 615                 620                 


Phe Gln Glu Asp Leu Glu Gln Ile Val Glu Trp Ser Thr Ile Phe Glu 
625                 630                 635                 640 


Asp Arg Gly Ile Phe Lys Ala Lys Leu Gln Ala Leu Gly Trp Leu Ser 
                645                 650                 655     


Glu Lys Gln Ile Gln Gln Leu Val Ala Lys Arg Tyr Lys Gly Trp Gly 
            660                 665                 670         


Arg Leu Ser Lys Lys Leu Leu Thr Gly Leu Lys Asn Ala Glu Gly Tyr 
        675                 680                 685             


Ser Ile Leu Asp Glu Met Trp Arg Ser Thr Glu Asn Phe Met Gln Ile 
    690                 695                 700                 


Gln Ser Arg Pro Glu Phe Ala Ala Leu Ile Gln Gln Ala Asn Glu Lys 
705                 710                 715                 720 


Gln Phe Glu Gly Asn Asp Pro Asp Asn Val Trp Glu Asn Ile Glu Asn 
                725                 730                 735     


Ile Leu Gly Asp Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Val Val Lys Val Val Gln Asp Ile Glu Lys Ala Val Gly Asn Pro 
        755                 760                 765             


Pro Glu Lys Ile Ala Ile Glu Phe Thr Arg Glu Ala Ala Ala Asn Pro 
    770                 775                 780                 


Gln Arg Thr Gln Ser Arg Leu Arg Thr Leu Glu Lys Leu Tyr Glu Ser 
785                 790                 795                 800 


Ala Glu Glu Val Val Asp Ala Gly Leu Thr Ala Glu Leu Ala Glu Phe 
                805                 810                 815     


Lys Glu Asn Lys His Val Leu Ser Asp Lys Tyr Tyr Leu Tyr Phe Thr 
            820                 825                 830         


Gln Leu Gly Arg Asp Val Tyr Thr Gly Asp Thr Ile Ser Leu Asp Lys 
        835                 840                 845             


Leu Asn Asp Tyr Asp Val Asp His Ile Leu Pro Gln Ser Phe Ile Lys 
    850                 855                 860                 


Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ile Arg Ala Val Asn Asn 
865                 870                 875                 880 


Gly Lys Ser Asp Asn Val Pro Ala Lys Met Phe Gly Lys Lys Met Gly 
                885                 890                 895     


Ser Phe Trp Arg His Leu Leu Asp Asn Gly Met Ile Ser Lys Arg Lys 
            900                 905                 910         


Tyr Asn Asn Leu Ile Thr Asp Pro Asp Asn Ile Ser Lys Tyr Ala Gln 
        915                 920                 925             


Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys 
    930                 935                 940                 


Leu Thr Ala Asn Ile Leu Asn Gly Ile Tyr Asp Lys Asp Thr Glu Ile 
945                 950                 955                 960 


Ile Glu Val Pro Ala Lys Met Asn Ser Gln Met Arg Lys Met Phe Asp 
                965                 970                 975     


Leu Val Lys Val Arg Glu Val Asn Asp Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Tyr Leu Thr Ile Phe Ile Gly Asn  Tyr Leu Tyr Lys Cys  Tyr Pro Lys 
        995                 1000                 1005             


Leu Arg  Pro Tyr Phe Val Tyr  Asp Asn Phe Lys Lys  Phe Gly Asn 
    1010                 1015                 1020             


Lys Glu  Asp Ile Gly His Lys  Arg Phe Asn Phe Leu  Gly Lys Ile 
    1025                 1030                 1035             


Glu Arg  Glu Lys Lys Val Val  Ala Pro Glu Thr Gly  Glu Ile Leu 
    1040                 1045                 1050             


Trp Ser  Asn Val Ala Pro Asn  Glu Thr Ile Lys Gln  Ile Lys Lys 
    1055                 1060                 1065             


Val Tyr  Asn Tyr Lys Phe Met  Ile Val Ser Arg Glu  Ile Thr Thr 
    1070                 1075                 1080             


Arg Arg  Ala Glu Leu Phe Asn  Gln Thr Val Tyr Pro  Lys Asn Tyr 
    1085                 1090                 1095             


Arg Gly  Lys Leu Ile Pro Ile  Lys Glu Asp Arg Pro  Thr Asp Leu 
    1100                 1105                 1110             


Tyr Gly  Gly Tyr Ser Gly Asn  Thr Asp Ala Tyr Leu  Ala Ile Val 
    1115                 1120                 1125             


Ala Leu  Glu Asp Lys Lys Lys  Gly Lys Tyr Phe Lys  Val Val Gly 
    1130                 1135                 1140             


Ile Pro  Thr Arg Ala Ala Ala  Lys Leu Glu Lys Leu  Lys Gln Gln 
    1145                 1150                 1155             


Asp Ser  Gln Gln Tyr Leu Gln  Ala Leu His Glu Val  Ile Ala Pro 
    1160                 1165                 1170             


Gln Phe  Thr Lys Ser Thr Lys  Lys Gly Ile Lys Lys  Thr Glu Phe 
    1175                 1180                 1185             


Glu Ile  Val Leu Asp Lys Val  His Tyr Arg Gln Leu  Val Gln Asp 
    1190                 1195                 1200             


Gly Pro  Val Lys Met Met Leu  Gly Ser Ser Thr Tyr  Lys Tyr Asn 
    1205                 1210                 1215             


Ala Lys  Gln Leu Val Leu Ser  Glu Lys Ala Leu 
    1220                 1225                 


<210>  784
<211>  1231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  784

Met Ile Thr Arg Ile Leu Gly Leu Asp Thr Gly Thr Asn Ser Leu Ala 
1               5                   10                  15      


Trp Ala Val Val Asp Arg Asp Ala Asn Gly Asn Tyr Thr Leu Val Lys 
            20                  25                  30          


Arg Gly Asp Val Ile Phe Thr Glu Gly Val Lys Ile Glu Lys Gly Ile 
        35                  40                  45              


Glu Ser Ser Lys Ala Ala Glu Arg Thr Ala His Lys Ala Leu Arg Val 
    50                  55                  60                  


His Tyr Ser Arg Arg Arg Leu His Lys Ile Asp Thr Leu Lys Val Leu 
65                  70                  75                  80  


Val Glu Leu Asp Leu Cys Pro Tyr Leu Ser Asn Glu Gln Leu Asn Leu 
                85                  90                  95      


Trp Lys Ala Lys Lys Thr Tyr Pro Leu Thr Asp Asp Phe Met Leu Trp 
            100                 105                 110         


Gln Arg Ser Gly Ser Ala Glu Glu Asn Asn Pro Tyr Tyr Cys Arg His 
        115                 120                 125             


Arg Cys Leu Thr Gln Lys Leu Asp Leu Thr Asp Gln Ser Gln Arg Phe 
    130                 135                 140                 


Leu Leu Gly Arg Ala Phe Tyr His Leu Ala Gln Arg Arg Gly Phe Leu 
145                 150                 155                 160 


Ser Asn Arg Leu Asp Thr Ser Ala Asp Ser Asp Glu Ser Gly Lys Val 
                165                 170                 175     


Lys Ser Gly Ile Ala Glu Leu Ser Val Glu Met Glu Lys Ala Gly Cys 
            180                 185                 190         


Glu Phe Leu Gly Glu Tyr Phe Tyr Gln Leu Tyr Lys Gln His Gly Asn 
        195                 200                 205             


Thr Ile Lys Leu Arg Ser Arg Tyr Thr Asp Arg Glu Glu His Tyr Lys 
    210                 215                 220                 


Lys Glu Phe Leu Ala Ile Cys Glu Lys Gln Glu Leu Asp Pro Lys Trp 
225                 230                 235                 240 


Val Glu Ala Leu Gln Arg Ala Ile Tyr Phe Gln Arg Pro Leu Lys Ser 
                245                 250                 255     


Gln Arg Lys Gly Val Gly Lys Cys Thr Phe Glu Pro Lys Lys Pro Arg 
            260                 265                 270         


Cys Ala Glu Ser His Pro Asp Tyr Glu Ala Phe Arg Met Leu Cys Phe 
        275                 280                 285             


Leu Asn Asn Val Lys Ile Gln Thr Pro Asn Asp Thr Ser Leu Arg Pro 
    290                 295                 300                 


Leu Asn Glu Asp Glu Arg Glu Lys Ile Trp Pro Leu Phe Phe Arg Lys 
305                 310                 315                 320 


Ser Lys Pro Asn Phe Asp Phe Glu Asp Ile Ala Lys Ala Leu Ala Gly 
                325                 330                 335     


Lys Asn Asn Tyr Ala Trp Tyr Lys Asp Ala Gly Glu Lys Pro Phe Lys 
            340                 345                 350         


Phe Asn Tyr Arg Met Gln Gln Gly Val Ser Gly Cys Pro Val Thr Ala 
        355                 360                 365             


Gln Leu Ile Asp Ile Phe Gly Ser Asp Trp Gln Thr Ala Ile Ala Glu 
    370                 375                 380                 


Thr Tyr Thr Ala Val Glu Lys Lys Asn Gly Thr Lys Thr Ile Asp Glu 
385                 390                 395                 400 


Met Ala Asp Asp Val Trp Asn Val Leu Tyr Ser Phe Ser Ser Lys Asp 
                405                 410                 415     


Lys Leu Lys Ala Phe Ala Thr Glu Lys Leu Gln Leu Asp Glu Ala Ser 
            420                 425                 430         


Ala Thr Lys Phe Ala Asn Ala Lys Leu Pro His Gly Phe Ala Ser Leu 
        435                 440                 445             


Ser Leu Lys Ala Ile Arg Asn Ile Leu Pro Phe Leu Arg Gln Gly Phe 
    450                 455                 460                 


Ile Tyr Ala His Ala Val His Leu Ala Lys Ile Pro Asp Ile Val Thr 
465                 470                 475                 480 


Lys Ala Val Trp Asn Asp Ala Asp Lys Arg Glu Met Ile Leu Ser Gly 
                485                 490                 495     


Leu Met Glu Ile Ile Glu Asn Tyr Asn Pro Lys Asp Arg Gly Val Glu 
            500                 505                 510         


Gly Thr Ile Asp Phe Cys Ile Lys Ser His Leu Gln Asp Val Ile Asp 
        515                 520                 525             


Leu Arg Pro Gly Ala Ala Asp Ala Leu Tyr His Pro Ser Met Ile Glu 
    530                 535                 540                 


Thr Tyr Pro Asp Ala Lys Leu Asn Lys Met Gly Val Phe Gln Leu Gly 
545                 550                 555                 560 


Ser Pro Arg Thr Asn Ala Ile Arg Asn Pro Met Ala Met Arg Ser Leu 
                565                 570                 575     


His Ile Leu Arg Ser Val Val Asn Gln Leu Leu Arg Asp Gly Ile Ile 
            580                 585                 590         


Asp Arg Asn Thr Glu Val His Val Glu Tyr Ala Arg Glu Leu Asn Asp 
        595                 600                 605             


Ala Asn Lys Arg Lys Ala Ile Ala Asp Tyr Gln Arg Glu Gln Glu Lys 
    610                 615                 620                 


Lys Arg Lys Gln Tyr Arg Asp Asp Ile Ile Ala Leu Tyr Lys Ser Glu 
625                 630                 635                 640 


Cys His Lys Glu Ile Glu Pro Thr Asp Thr Asp Ile Leu Lys Phe Gln 
                645                 650                 655     


Leu Trp Glu Glu Gln Asp His Leu Cys Leu Tyr Thr Gly Thr Gln Ile 
            660                 665                 670         


Gly Ile Cys Asp Phe Leu Gly Asp Asn Pro Lys Phe Asp Ile Glu His 
        675                 680                 685             


Thr Val Pro Arg Ser Val Gly Gly Asp Ser Thr Gln Met Asn Leu Thr 
    690                 695                 700                 


Leu Cys Asp Ala Thr Phe Asn Arg Gln Val Lys Gly Ala Gln Leu Pro 
705                 710                 715                 720 


Ser Gln Leu Ser Asn His Ala Asp Ile Leu Val Arg Ile Asp His Trp 
                725                 730                 735     


Lys Ser Lys Cys Glu Asp Leu Arg Lys Gln Leu Asp Lys Ile Arg Thr 
            740                 745                 750         


Tyr Gly Gly Met Glu Lys Ala Met Lys Asp Arg Leu Ile Gln Lys Arg 
        755                 760                 765             


His Arg Leu Lys Met Asp Leu Asp Tyr Trp Gln Gly Lys Tyr Ser Arg 
    770                 775                 780                 


Phe Thr Met Thr Glu Val Pro Glu Gly Phe Ser Arg Arg Gln Gly Ala 
785                 790                 795                 800 


Gly Ile Gly Leu Val Ser Lys Tyr Ala Gly Leu Phe Leu Lys Ser Leu 
                805                 810                 815     


Phe His Asn Glu Gln Asn Pro Asn Lys Ser Asn Val Tyr Val Val Lys 
            820                 825                 830         


Gly Val Thr Thr Ala Glu Phe Arg Arg Met Trp Gly Leu Gln Gly Glu 
        835                 840                 845             


Tyr Glu Lys Lys Ser Arg Leu Asn His Cys His His Cys Ile Asp Ala 
    850                 855                 860                 


Ile Thr Ile Ala Cys Ile Gly Lys Ala Glu Tyr Asp Lys Met Ala Gln 
865                 870                 875                 880 


Tyr Tyr His Asp Glu Glu Leu Phe Gln Trp Arg Gly Thr Thr Thr Lys 
                885                 890                 895     


Pro His Phe Asn Lys Pro Trp Pro Thr Phe Thr Glu Asp Val Leu Ala 
            900                 905                 910         


Ile Glu Gln Ser Leu Leu Val Lys His Leu Thr Lys Asp Asn Met Pro 
        915                 920                 925             


Lys Gln Ala Lys Lys Arg Val Arg Thr Ala Thr Gly Lys Tyr Leu Ala 
    930                 935                 940                 


Gln Gly Asp Thr Ala Arg Gly Ser Leu His Lys Asp Thr Tyr Tyr Gly 
945                 950                 955                 960 


Ala Ile Glu Gln Asp Gly Glu Ile Arg Tyr Val Leu Arg Lys Pro Leu 
                965                 970                 975     


Asn Lys Phe Glu Asn Glu Ala Glu Leu Glu Lys Ile Val Asp Gln Ala 
            980                 985                 990         


Val Lys Glu Lys Ile Lys Glu Ala  Ile Ala Gly Lys Asn  Phe Lys Thr 
        995                 1000                 1005             


Ala Ile  Thr Glu Pro Ile Phe  Met Asn Lys Glu Lys  Gly Ile Arg 
    1010                 1015                 1020             


Ile Asn  Lys Val Arg Tyr Tyr  Val Lys Ala Gln Glu  Arg Leu Lys 
    1025                 1030                 1035             


Ile Arg  Gln Gln Arg Asp Leu  Ser Ser Lys Glu Tyr  Lys Gln Phe 
    1040                 1045                 1050             


Phe Cys  Val Glu Asn Glu Ser  Asn Tyr Leu Leu Ala  Ile Tyr Glu 
    1055                 1060                 1065             


Gly Glu  Leu Lys Gly Lys Lys  Glu Arg Asp Phe Cys  Val Val Asn 
    1070                 1075                 1080             


Asn Leu  Glu Ser Ala Ala Cys  Leu Lys Arg Ser Asn  Lys Gly Lys 
    1085                 1090                 1095             


Ala Ala  Pro Leu Val Pro Glu  Arg Ser Glu Glu Arg  Asn Leu Pro 
    1100                 1105                 1110             


Leu Leu  Ala Val Leu Lys Arg  Gly Thr Gln Val Leu  Phe Tyr Glu 
    1115                 1120                 1125             


Asn Ser  Pro Glu Glu Ile Leu  Phe Asp Asn Pro Gln  Asp Leu Val 
    1130                 1135                 1140             


Arg Arg  Leu Tyr Lys Val Thr  Gly Ile Asn Thr Lys  Lys Asp Val 
    1145                 1150                 1155             


Lys Asn  Gly Lys Thr Tyr Leu  Tyr Gly Asn Ile Glu  Leu Arg Phe 
    1160                 1165                 1170             


His Gln  Asn Ala Gln Lys Lys  Asp Asp Val Lys Met  Gln Ser Gly 
    1175                 1180                 1185             


Val Tyr  Lys Asn Asn Glu Val  Leu Arg Gln Gly Ile  Val Leu Ser 
    1190                 1195                 1200             


His Lys  Gln Phe Lys Ala Leu  Val Glu Gly Val Asp  Phe Glu Phe 
    1205                 1210                 1215             


Asn Val  Leu Gly Glu Ile Lys  Lys Lys Glu Arg His  Ala 
    1220                 1225                 1230     


<210>  785
<211>  1232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  785

Met Thr Lys Thr Arg Ile Leu Gly Leu Asp Thr Gly Thr Asn Ser Leu 
1               5                   10                  15      


Gly Trp Ala Ile Val Asp Arg Asp Glu Val Ser Gly Gln Tyr Glu Leu 
            20                  25                  30          


Val Asp Arg Gly Asp Leu Ile Phe Thr Glu Gly Val Lys Ile Glu Lys 
        35                  40                  45              


Gly Ile Glu Ser Ser Lys Ala Ser Glu Arg Thr Gln His Arg Ala Leu 
    50                  55                  60                  


Arg Lys Gln Tyr Phe Arg Arg Arg Leu Arg Lys Ile Glu Val Leu Arg 
65                  70                  75                  80  


Val Leu Thr Phe Tyr His Leu Cys Pro Pro Val Ser Glu Glu Glu Leu 
                85                  90                  95      


His Leu Trp His Val Lys Lys Ile Tyr Pro Val Glu Asn His Glu Phe 
            100                 105                 110         


Met Ile Trp Gln Arg Thr Asn Asp Asn Glu Gly Lys Asn Pro Tyr Tyr 
        115                 120                 125             


Tyr Arg His Ile Cys Leu His Thr Lys Leu Asn Leu Thr Leu Met Lys 
    130                 135                 140                 


Asp Arg Tyr Ile Leu Gly Arg Ala Leu Tyr His Leu Ser Gln Arg Arg 
145                 150                 155                 160 


Gly Phe Leu Ser Asn Arg Leu Asp Gln Ser Gln Asp Glu Asp Asp Lys 
                165                 170                 175     


Gly Thr Val Lys Thr Gly Ile Ser Ser Leu Ser Lys Asp Met Asn Asp 
            180                 185                 190         


Ala Gly Cys Thr Tyr Leu Gly Asp Tyr Phe Tyr Lys Leu Tyr Ser Glu 
        195                 200                 205             


Asn Gly Asn Arg Val Arg Ile Arg Asn His Tyr Thr Ala Arg Asn Glu 
    210                 215                 220                 


His Tyr Leu Ala Glu Phe Glu Ala Ile Cys Gln Val Gln Lys Leu Glu 
225                 230                 235                 240 


Pro Glu Leu Val Glu Ala Leu Arg Arg Ala Ile Phe Phe Gln Arg Pro 
                245                 250                 255     


Leu Lys Ser Gln Arg Lys Gly Val Gly Lys Cys Thr Phe Glu Pro Arg 
            260                 265                 270         


Lys Pro Arg Cys Ala Asp Ser His Pro Asp Tyr Glu Glu Phe Arg Lys 
        275                 280                 285             


Leu Cys Phe Ile Asn Asn Ile Lys Ile Gln Thr Ser Asp Asp Asp Thr 
    290                 295                 300                 


Met Arg Pro Leu Asn Lys Glu Glu Arg Gln Arg Ile Asn Ser Leu Phe 
305                 310                 315                 320 


Tyr Arg Val Ser Lys Ala Asn Phe Tyr Phe Glu Glu Ile Ala Lys Leu 
                325                 330                 335     


Leu Gly Gly Arg Asn Asn Tyr Cys Tyr Phe Lys Asp Glu Val Gln Lys 
            340                 345                 350         


Asp Phe Lys Phe Asn Tyr Arg Met Ser Gln Ser Val Ser Gly Cys Pro 
        355                 360                 365             


Thr Thr Ala Gln Leu Arg Asn Ile Phe Gly Glu Asp Trp Lys Thr Gly 
    370                 375                 380                 


Ile Ala Glu Thr Tyr Gln His Ser Ala Lys Lys Asp Gly Ser Leu Lys 
385                 390                 395                 400 


Thr Val Asp Glu Met Ala Asp Asp Ile Trp Asn Val Leu Tyr Ser Phe 
                405                 410                 415     


Ser Ser Lys Asp Cys Leu Tyr Asn Phe Ala Val Asn Lys Leu Gln Leu 
            420                 425                 430         


Glu Glu Asp Lys Ala Lys Lys Phe Ser Lys Ile Lys Leu Ser His Gly 
        435                 440                 445             


Phe Ala Ser Ile Ser Leu Asn Ala Ile His Lys Ile Leu Pro Phe Leu 
    450                 455                 460                 


Arg Met Gly Met Ile Tyr Ser His Ala Val Leu Phe Ala Asn Ile Pro 
465                 470                 475                 480 


Thr Ile Val Gly Met Asp Val Trp Asn Asp Glu Ser Lys Arg Gln His 
                485                 490                 495     


Ile Met Thr Glu Leu Ser Asp Leu Val Phe Asn Tyr Asp Pro Lys Asn 
            500                 505                 510         


Arg Glu Val Glu Gly Thr Ile Glu Met Cys Ile Lys Asp Phe Leu Leu 
        515                 520                 525             


Asn Asn Tyr Asp Leu Pro Ala Gly Ala Val Cys Arg Leu Tyr His Pro 
    530                 535                 540                 


Ser Met Ile Asp Thr Tyr Pro Asp Ala Lys Ala Asn Lys Tyr Gly Val 
545                 550                 555                 560 


Leu Gln Leu Gly Ser Pro Ala Thr Asn Ala Ile Arg Asn Pro Met Ala 
                565                 570                 575     


Met Arg Ser Leu His Glu Leu Arg His Val Val Asn Leu Leu Leu Arg 
            580                 585                 590         


Lys Gly Lys Ile Asp Gln Asn Thr Glu Val His Val Glu Tyr Ala Arg 
        595                 600                 605             


Glu Leu Asn Asp Ala Asn Lys Arg Lys Ala Ile Ala Asp Arg Gln Arg 
    610                 615                 620                 


Glu Leu Asp Lys Leu His Lys Lys Tyr His Asp Asp Ile Ile Thr Leu 
625                 630                 635                 640 


Tyr Lys Glu Glu Thr Gly Lys Asp Ile Asn Pro Thr Gln Ala Asp Ile 
                645                 650                 655     


Leu Lys Phe Gln Leu Trp Glu Glu Gln Glu His Val Cys Leu Tyr Thr 
            660                 665                 670         


Gly Arg Gln Ile Gly Ile Ser Lys Phe Leu Gly Pro Asp Pro Glu Phe 
        675                 680                 685             


Asp Ile Glu His Thr Val Pro Arg Ser Val Gly Gly Asp Ser Thr Gln 
    690                 695                 700                 


Met Asn Met Thr Leu Cys Asp Arg Arg Phe Asn Arg Asp Val Lys Lys 
705                 710                 715                 720 


Ala Met Leu Pro Ser Gln Leu Pro Asn Tyr Gln Glu Ile Leu Pro Arg 
                725                 730                 735     


Ile Glu Lys Trp Lys Lys Lys Tyr Glu Lys Leu Thr Lys Asp Ile Asp 
            740                 745                 750         


His Cys Ile Thr His Ser Gly Met Thr Lys Glu Ala Lys Asp Ser Ile 
        755                 760                 765             


Ile Gln Lys Arg His Arg Leu Thr Leu Glu His Asp Tyr Trp Arg Asn 
    770                 775                 780                 


Lys Tyr Ser Arg Phe Thr Met Thr Glu Val Pro Glu Gly Phe Ser Arg 
785                 790                 795                 800 


Arg Gln Gly Ala Gly Ile Gly Leu Val Ser Lys Tyr Ala Gly Leu Phe 
                805                 810                 815     


Leu Lys Ser Leu Phe His Asp Pro Thr Asp Arg Arg Arg Ser His Val 
            820                 825                 830         


Tyr Val Val Lys Gly Thr Thr Thr Ala Glu Phe Arg Lys Met Trp Gly 
        835                 840                 845             


Leu Gln Ser Glu Tyr Glu Lys Lys Ser Arg Asp Asn His Val His His 
    850                 855                 860                 


Cys Val Asp Ala Ile Thr Ile Ala Cys Ile Gly Lys Asn Glu Tyr Asn 
865                 870                 875                 880 


Lys Met Ala Ser Phe Tyr His Asp Glu Glu Ala Phe Glu Arg Gly Glu 
                885                 890                 895     


Cys Glu Lys Pro His Phe Asp Lys Pro Trp Pro Thr Phe Thr Gln Asp 
            900                 905                 910         


Val Leu Gly Leu Glu Lys Arg Leu Ile Val Val His Ser Thr Gln Asp 
        915                 920                 925             


Asn Met Pro Lys His Ala Lys Lys Tyr Val Arg Thr Pro Arg Gly Lys 
    930                 935                 940                 


Phe Ile Ala Lys Gly Asp Cys Ala Arg Gly Ser Leu His Leu Asp Thr 
945                 950                 955                 960 


Tyr Tyr Gly Ala Ile Lys His Lys Asp Asp Thr Glu Pro Arg Tyr Val 
                965                 970                 975     


Leu Arg Arg Glu Leu Ser Ser Phe Asp Ser Val Asn Asp Leu Asp Ile 
            980                 985                 990         


Ile Val Asp Asp Ala Val Arg Glu  Lys Ile Lys Glu Ala  Val Lys Gly 
        995                 1000                 1005             


Lys Ser  Gly Lys Ala Phe Lys  Glu Ala Leu Ala Asn  Val Tyr Met 
    1010                 1015                 1020             


Asn Lys  Glu Lys Asp Ile Arg  Ile Lys Lys Val Arg  Cys Tyr Val 
    1025                 1030                 1035             


Pro Ser  Val Lys Arg Pro Ile  Asp Ile Arg His Gln  Arg Asp Met 
    1040                 1045                 1050             


Ser Arg  Phe Asp Tyr Lys Arg  Gln Phe His Val Ala  Asn Asp Gly 
    1055                 1060                 1065             


Asn Tyr  Met Met Ala Ile Tyr  Glu Gly Val Val Lys  Gly Lys Leu 
    1070                 1075                 1080             


Lys Arg  Asp Phe Glu Leu Val  Lys Asn Ile Glu Ala  Ala Ser Tyr 
    1085                 1090                 1095             


Phe Lys  Val Ser Gln Asp Asn  Glu Ser Phe Ser Ser  Ile Val Pro 
    1100                 1105                 1110             


Glu Lys  Ser Ser Asn Gly Phe  Pro Leu Lys Ala Val  Leu Lys Ile 
    1115                 1120                 1125             


Gly Thr  His Val Leu Leu Tyr  Glu Asn Thr Ala Ser  Glu Ile Asp 
    1130                 1135                 1140             


Phe Ser  Asp Pro Val Asp Leu  Thr Arg Arg Leu Tyr  Lys Val Val 
    1145                 1150                 1155             


Gly Leu  Ser Ser Ala Ile Ile  Asn Asn Cys Ser Tyr  Gly Phe Ile 
    1160                 1165                 1170             


Thr Leu  Arg Phe His Gln Glu  Ala Arg Gln Ala Lys  Asn Leu Lys 
    1175                 1180                 1185             


Leu Thr  Lys Gly Val Phe Lys  Asn Val Asp Asn Tyr  Arg Gly Ala 
    1190                 1195                 1200             


Ile Ile  Leu Tyr His Thr Gln  Leu Lys Ala Leu Val  Glu Gly Tyr 
    1205                 1210                 1215             


Asp Phe  Asp Ile Asn Ile Leu  Gly Glu Ile Thr Ser  Lys Gln 
    1220                 1225                 1230         


<210>  786
<211>  1234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  786

Met Pro Asn Phe Trp Ser Ile Phe Thr Glu Asn Leu Gln Thr Tyr Leu 
1               5                   10                  15      


Met Lys Thr Arg Ile Leu Gly Leu Asp Thr Gly Thr Asn Ser Leu Gly 
            20                  25                  30          


Trp Ala Val Val Asp Arg Asp Glu Asn Asn Gln Tyr Thr Pro Ile Cys 
        35                  40                  45              


Arg Gly Asp Leu Ile Phe Thr Glu Gly Val Lys Gln Glu Lys Gly Ile 
    50                  55                  60                  


Glu Phe Ser Leu Ala Ala Glu Arg Thr Ser Tyr Arg Ala Ser Arg Arg 
65                  70                  75                  80  


His Tyr Phe Arg Arg Arg Leu Arg Lys Val Glu Val Leu Lys Val Leu 
                85                  90                  95      


Val Lys His Lys Leu Cys Pro Pro Leu Ser Asp Glu Ala Leu Asn Arg 
            100                 105                 110         


Trp His Thr Lys Lys Glu Tyr Pro Leu Asp Glu Asp Phe Ile Leu Trp 
        115                 120                 125             


Gln Arg Thr Asn Glu Ala Asp Asn Lys Asn Pro Tyr His Asp Arg His 
    130                 135                 140                 


Ile Cys Leu His Arg Lys Leu Asn Leu Lys Asp Ser Gln Thr Asp Arg 
145                 150                 155                 160 


Tyr Thr Leu Gly Arg Ala Leu Tyr His Leu Thr Gln Arg Arg Gly Phe 
                165                 170                 175     


Leu Ser Asn Arg Leu Asp Gln Asn Glu Glu Ala Glu Glu Thr Gly Lys 
            180                 185                 190         


Val Lys Ser Ala Ile Ser Glu Leu Ser Lys Glu Met Lys Glu Ala Gly 
        195                 200                 205             


Cys Glu Phe Leu Gly Asp Tyr Phe Tyr Leu Leu Tyr Arg Thr Glu Gly 
    210                 215                 220                 


Asn Arg Val Arg Ile Arg Ser Arg Tyr Thr Asp Arg Glu Lys His Tyr 
225                 230                 235                 240 


Leu Gln Glu Phe Lys Glu Ile Cys Arg Val Gln Glu Leu Asp Glu Asp 
                245                 250                 255     


Leu Ala Lys Ala Leu Glu Lys Thr Ile Phe Phe Gln Arg Pro Leu Lys 
            260                 265                 270         


Ser Gln Arg Gln Gly Val Gly Lys Cys Thr Met Glu Lys Gly His Ser 
        275                 280                 285             


Arg Cys Ala Val Ser His Pro Leu Tyr Glu Glu Tyr Arg Leu Leu Cys 
    290                 295                 300                 


Tyr Leu Asn Asn Ile Arg Ile Lys Thr Pro Ser Asp Asp Arg Leu Arg 
305                 310                 315                 320 


Pro Leu Asn Ala Glu Glu Arg Glu Lys Ile Thr Pro Leu Phe Tyr Lys 
                325                 330                 335     


Glu Lys Asn Phe Asp Phe Glu Lys Ile Ala Lys Lys Leu Ala Gly Lys 
            340                 345                 350         


Thr Pro Phe Gly Trp Ile Arg Asp Ala Glu Asp Trp Pro Tyr Gln Phe 
        355                 360                 365             


Asn Tyr Arg Met Thr Gln Glu Ala Pro Ala Cys Pro Thr Thr Ala Gln 
    370                 375                 380                 


Leu Ile Lys Val Phe Gly Glu Asp Trp Lys Ala Ala Leu Ala Glu Thr 
385                 390                 395                 400 


Tyr Thr Met Ser Ala Lys Gln Asp Gly Thr Leu Lys Thr Glu Asp Glu 
                405                 410                 415     


Ile Cys Thr Asp Ile Trp Asn Val Leu Tyr Ser Phe Ser Ser Lys Glu 
            420                 425                 430         


Lys Leu Lys Glu Phe Gly Leu Lys Lys Leu Gln Leu Asp Glu Val Gln 
        435                 440                 445             


Ala Lys Ala Phe Ser Glu Ile Arg Leu Thr Arg Asp Phe Ala Ser Leu 
    450                 455                 460                 


Ser Leu Lys Ala Ile Arg Lys Ile Leu Phe Phe Leu Arg Thr Gly Glu 
465                 470                 475                 480 


Asp Tyr Ser Lys Ala Ile Phe Leu Ala Asn Ile Pro Thr Ile Val Gly 
                485                 490                 495     


Glu Glu Ile Trp Asn Asp Ile Glu Arg Arg Ser Arg Leu Leu Tyr Lys 
            500                 505                 510         


Val Asp Leu Ala Leu Arg Pro Gln Glu Glu Arg Asp Ala Glu Glu His 
        515                 520                 525             


Arg Thr Ile Ala Glu Arg Ile Lys Ala Ala Val Ala Ala Glu Val Pro 
    530                 535                 540                 


Asp Ala Pro Ile Asp Leu Leu Tyr His Pro Ser Met Ile Glu Ala Tyr 
545                 550                 555                 560 


Pro Asp Ala Lys Arg Asn Glu Arg Phe Asp Cys Tyr Gln Leu Gly Ser 
                565                 570                 575     


Pro Lys Thr Asn Ala Val Arg Asn Pro Met Ala Met Arg Ser Leu His 
            580                 585                 590         


Met Val Arg Lys Val Ile Asn His Leu Leu Arg Lys His Ile Ile Asp 
        595                 600                 605             


Glu Lys Thr Glu Ile His Ile Glu Tyr Ala Arg Glu Leu Asn Asp Ala 
    610                 615                 620                 


Asn Lys Arg Gln Ala Ile Ala Asp Trp Gln Arg Glu Leu Ser Lys Arg 
625                 630                 635                 640 


His Thr Ala Tyr Ala Gln Asn Ile Arg Gln Leu Tyr Lys Ala Glu Thr 
                645                 650                 655     


Gly Leu Val Ile Glu Pro Thr Lys Ala Asp Ile Leu Lys Tyr Gln Leu 
            660                 665                 670         


Trp Glu Glu Gln Gln His Val Cys Leu Tyr Thr Gly Lys Lys Ile Gly 
        675                 680                 685             


Ile Ala Asp Phe Leu Gly Ala Asn Pro Lys Phe Asp Ile Glu His Thr 
    690                 695                 700                 


Ile Pro Arg Ser Arg Gly Gly Asp Ser Thr Gln Glu Asn Leu Thr Leu 
705                 710                 715                 720 


Cys Glu Ser Lys Phe Asn Arg Asp Ile Lys Arg Asp Lys Leu Pro Thr 
                725                 730                 735     


Glu Leu Ala Asn Tyr Glu Glu Ile Leu Ala Arg Ile Glu His Trp Lys 
            740                 745                 750         


Lys Gln Cys Tyr Ala Leu Lys Leu Lys Arg Asp Gly Ile Arg Thr His 
        755                 760                 765             


Ser Gly Leu Glu Lys Ser Ile Lys Asp Ser Arg Ile Arg Arg Arg His 
    770                 775                 780                 


Leu Val Gly Leu Glu Tyr Gly Tyr Leu Lys Gly Lys Tyr Asp Arg Phe 
785                 790                 795                 800 


Thr Met Lys Glu Val Pro Glu Gly Phe Ser Arg Arg Gln Gly Ala Gly 
                805                 810                 815     


Ile Gly Leu Ile Ser Lys Tyr Ala Gly Leu Phe Leu Lys Ser Leu Phe 
            820                 825                 830         


Lys Asp Pro Lys Asn Pro Asn Arg Ser Asn Val Tyr Val Val Lys Gly 
        835                 840                 845             


Ala Ile Thr Ala Glu Phe Arg Lys Leu Trp Gly Leu Gln Gln Ala Asp 
    850                 855                 860                 


Glu Ala Lys Ser Arg Glu Asn His Thr His His Cys Ile Asp Ala Tyr 
865                 870                 875                 880 


Thr Ile Ala Cys Ile Gly Pro Cys Glu Tyr Ala Ala Leu Ala Ala Tyr 
                885                 890                 895     


Tyr Arg Ser Asp Glu Glu Phe Lys Tyr Gly Arg Arg Arg Glu Lys Pro 
            900                 905                 910         


Gln Phe Glu Lys Pro Trp Pro Thr Phe Thr Glu Asp Leu Leu Lys Leu 
        915                 920                 925             


Gln Glu Glu Leu Leu Ile Val His Gln Thr Thr Asp Lys Leu Gly Lys 
    930                 935                 940                 


Arg Asp Arg Arg Lys Val Lys Thr Pro Arg Gly Lys Phe Leu Thr Gly 
945                 950                 955                 960 


Gly Asp Ser Ala Arg Gly Arg Leu His Gln Glu Thr Tyr Tyr Gly Ala 
                965                 970                 975     


Ile Asn Tyr Asp Gly Asn Ile Lys Tyr Val Val Arg Lys Pro Leu Asp 
            980                 985                 990         


Ser Leu Thr Glu Lys Asp Leu Asp  Lys Ile Val Asp Glu  Thr Val Met 
        995                 1000                 1005             


Gly Ile  Val Lys Gly Ala Val  Glu Ala Lys Gly Ser  Leu Lys Glu 
    1010                 1015                 1020             


Ala Leu  Ala Asp Gly Ile Tyr  Met Asn Lys Glu Lys  Gly Ile Leu 
    1025                 1030                 1035             


Ile Lys  Arg Val Arg Ile Phe  Ser Asp Val Ser Glu  Pro Ile Asn 
    1040                 1045                 1050             


Leu Lys  Gln Leu Arg Asp Val  Ser Thr Lys Glu Tyr  Lys Arg His 
    1055                 1060                 1065             


Ile His  Leu Arg Lys Asp Glu  Asn Tyr Leu Phe Ala  Ile Tyr Glu 
    1070                 1075                 1080             


Gly Ile  Asp Lys Lys Lys Asn  Lys Lys Gln Thr Glu  Thr Ala Thr 
    1085                 1090                 1095             


Val Asp  Tyr Leu Asp Ala Ala  Lys Phe Tyr Lys Asp  Ser Lys Arg 
    1100                 1105                 1110             


Arg Asp  Lys Ala Asn Ser Leu  Val Gly Asp Tyr Ser  Lys Lys Asn 
    1115                 1120                 1125             


Lys Leu  Pro Leu Lys Cys Ile  Leu Lys Lys Arg Gln  Met Val Leu 
    1130                 1135                 1140             


Phe Tyr  Glu His Ser Pro Glu  Glu Ile Arg Phe Lys  Asp Lys Lys 
    1145                 1150                 1155             


Asp Leu  Ser Arg Arg Leu Tyr  Ile Val Thr Ser Ile  Ala Lys Asp 
    1160                 1165                 1170             


Gly Arg  Val Lys Phe Leu His  His Gln Ala Ala Thr  Ala Thr Gly 
    1175                 1180                 1185             


Ile Gln  Ser Ser Pro Ser Pro  Phe Thr Asn Asp Gly  Glu His Gln 
    1190                 1195                 1200             


Pro Tyr  Tyr Arg Leu Ser Ala  Ser Asn Ile Lys Val  Leu Val Ala 
    1205                 1210                 1215             


Gly Ile  Asp Phe Asp Ile Asn  Ile Leu Gly Glu Ile  Thr Pro Lys 
    1220                 1225                 1230             


Leu 
    


<210>  787
<211>  1236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  787

Met Tyr Phe Tyr Lys Asn Lys Glu Asn Lys Leu Asn Lys Lys Val Val 
1               5                   10                  15      


Leu Gly Leu Asp Leu Gly Ile Ala Ser Val Gly Trp Cys Leu Thr Asp 
            20                  25                  30          


Ile Ser Gln Lys Glu Asp Asn Lys Phe Pro Ile Ile Leu His Gly Val 
        35                  40                  45              


Arg Leu Phe Glu Thr Val Asp Asp Ser Asp Asp Lys Leu Leu Asn Glu 
    50                  55                  60                  


Thr Arg Arg Lys Lys Arg Gly Gln Arg Arg Arg Asn Arg Arg Leu Phe 
65                  70                  75                  80  


Thr Arg Lys Arg Asp Phe Ile Lys Tyr Leu Ile Asp Asn Asn Ile Ile 
                85                  90                  95      


Glu Leu Glu Phe Asp Lys Asn Pro Lys Ile Leu Val Arg Asn Phe Ile 
            100                 105                 110         


Glu Lys Tyr Ile Asn Pro Phe Ser Lys Asn Leu Glu Leu Lys Tyr Lys 
        115                 120                 125             


Ser Val Thr Asn Leu Pro Ile Gly Phe His Asn Leu Arg Lys Ala Ala 
    130                 135                 140                 


Ile Asn Glu Lys Tyr Lys Leu Asp Lys Ser Glu Leu Ile Val Leu Leu 
145                 150                 155                 160 


Tyr Phe Tyr Leu Ser Leu Arg Gly Ala Phe Phe Asp Asn Pro Glu Asp 
                165                 170                 175     


Thr Lys Ser Lys Glu Met Asn Lys Asn Glu Ile Glu Ile Phe Asp Lys 
            180                 185                 190         


Asn Glu Ser Ile Lys Asn Ala Glu Phe Pro Ile Asp Lys Ile Ile Glu 
        195                 200                 205             


Phe Tyr Lys Ile Ser Gly Lys Ile Arg Ser Thr Ile Asn Leu Lys Phe 
    210                 215                 220                 


Gly His Gln Asp Tyr Leu Lys Glu Ile Lys Gln Val Phe Glu Lys Gln 
225                 230                 235                 240 


Asn Ile Asp Phe Met Asn Tyr Glu Lys Phe Ala Met Glu Glu Lys Ser 
                245                 250                 255     


Phe Phe Ser Arg Ile Arg Asn Tyr Ser Glu Gly Pro Gly Asn Glu Lys 
            260                 265                 270         


Ser Phe Ser Lys Tyr Gly Leu Tyr Ala Asn Glu Asn Gly Asn Pro Glu 
        275                 280                 285             


Leu Ile Ile Asn Glu Lys Gly Gln Lys Ile Tyr Thr Lys Ile Phe Lys 
    290                 295                 300                 


Thr Leu Trp Glu Ser Lys Ile Gly Lys Cys Ser Tyr Asp Lys Lys Leu 
305                 310                 315                 320 


Tyr Arg Ala Pro Lys Asn Ser Phe Ser Ala Lys Val Phe Asp Ile Thr 
                325                 330                 335     


Asn Lys Leu Thr Asp Trp Lys His Lys Asn Glu Tyr Ile Ser Glu Arg 
            340                 345                 350         


Leu Lys Arg Lys Ile Leu Leu Ser Arg Phe Leu Asn Lys Asp Ser Lys 
        355                 360                 365             


Ser Ala Val Glu Lys Ile Leu Lys Glu Glu Asn Ile Lys Phe Glu Asn 
    370                 375                 380                 


Leu Ser Glu Ile Ala Tyr Asn Lys Asp Asp Asn Lys Ile Asn Leu Pro 
385                 390                 395                 400 


Ile Ile Asn Ala Tyr His Ser Leu Thr Thr Ile Phe Lys Lys His Leu 
                405                 410                 415     


Ile Asn Phe Glu Asn Tyr Leu Ile Ser Asn Glu Asn Asp Leu Ser Lys 
            420                 425                 430         


Leu Met Ser Phe Tyr Lys Gln Gln Ser Glu Lys Leu Phe Val Pro Asn 
        435                 440                 445             


Glu Lys Gly Ser Tyr Glu Ile Asn Gln Asn Asn Asn Val Leu His Ile 
    450                 455                 460                 


Phe Asp Ala Ile Ser Asn Ile Leu Asn Lys Phe Ser Thr Ile Gln Asp 
465                 470                 475                 480 


Arg Ile Arg Ile Leu Glu Gly Tyr Phe Glu Phe Ser Asn Leu Lys Lys 
                485                 490                 495     


Asp Val Lys Ser Ser Glu Ile Tyr Ser Glu Ile Ala Lys Leu Arg Glu 
            500                 505                 510         


Phe Ser Gly Thr Ser Ser Leu Ser Phe Gly Ala Tyr Tyr Lys Phe Ile 
        515                 520                 525             


Pro Asn Leu Ile Ser Glu Gly Ser Lys Asn Tyr Ser Thr Ile Ser Tyr 
    530                 535                 540                 


Glu Glu Lys Ala Leu Gln Asn Gln Lys Asn Asn Phe Ser His Ser Asn 
545                 550                 555                 560 


Leu Phe Glu Lys Thr Trp Val Glu Asp Leu Ile Ala Ser Pro Thr Val 
                565                 570                 575     


Lys Arg Ser Leu Arg Gln Thr Met Asn Leu Leu Lys Glu Ile Phe Lys 
            580                 585                 590         


Tyr Ser Glu Lys Asn Asn Leu Glu Ile Glu Lys Ile Val Val Glu Val 
        595                 600                 605             


Thr Arg Ser Ser Asn Asn Lys His Glu Arg Lys Lys Ile Glu Gly Ile 
    610                 615                 620                 


Asn Lys Tyr Arg Lys Glu Lys Tyr Glu Glu Leu Lys Lys Val Tyr Asp 
625                 630                 635                 640 


Leu Pro Asn Glu Asn Thr Thr Leu Leu Lys Lys Leu Trp Leu Leu Arg 
                645                 650                 655     


Gln Gln Gln Gly Tyr Asp Ala Tyr Ser Leu Arg Lys Ile Glu Ala Asn 
            660                 665                 670         


Asp Val Ile Asn Lys Pro Trp Asn Tyr Asp Ile Asp His Ile Val Pro 
        675                 680                 685             


Arg Ser Ile Ser Phe Asp Asp Ser Phe Ser Asn Leu Val Ile Val Asn 
    690                 695                 700                 


Lys Leu Asp Asn Ala Lys Lys Ser Asn Asp Leu Ser Ala Lys Gln Phe 
705                 710                 715                 720 


Ile Glu Lys Ile Tyr Gly Ile Glu Lys Leu Lys Glu Ala Lys Glu Asn 
                725                 730                 735     


Trp Gly Asn Trp Tyr Leu Arg Asn Ala Asn Gly Lys Ala Phe Asn Asp 
            740                 745                 750         


Lys Gly Lys Phe Ile Lys Leu Tyr Thr Ile Asp Asn Leu Asp Glu Phe 
        755                 760                 765             


Asp Asn Ser Asp Phe Ile Asn Arg Asn Leu Ser Asp Thr Ser Tyr Ile 
    770                 775                 780                 


Thr Asn Ala Leu Val Asn His Leu Thr Phe Ser Asn Ser Lys Tyr Lys 
785                 790                 795                 800 


Tyr Ser Val Val Ser Val Asn Gly Lys Gln Thr Ser Asn Leu Arg Asn 
                805                 810                 815     


Gln Ile Ala Phe Val Gly Ile Lys Asn Asn Lys Glu Thr Glu Arg Glu 
            820                 825                 830         


Trp Lys Arg Pro Glu Gly Phe Lys Ser Ile Asn Ser Asn Asp Phe Leu 
        835                 840                 845             


Ile Arg Glu Glu Gly Lys Asn Asp Val Lys Asp Asp Val Leu Ile Lys 
    850                 855                 860                 


Asp Arg Ser Phe Asn Gly His His Ala Glu Asp Ala Tyr Phe Ile Thr 
865                 870                 875                 880 


Ile Ile Ser Gln Tyr Phe Arg Ser Phe Lys Arg Ile Glu Arg Leu Asn 
                885                 890                 895     


Val Asn Tyr Arg Lys Glu Thr Arg Glu Leu Asp Asp Leu Glu Lys Asn 
            900                 905                 910         


Asn Ile Lys Phe Lys Glu Lys Ala Ser Phe Asp Asn Phe Leu Leu Ile 
        915                 920                 925             


Asn Ala Leu Asp Glu Leu Asn Glu Lys Leu Asn Gln Met Arg Phe Ser 
    930                 935                 940                 


Arg Met Val Ile Thr Lys Lys Asn Thr Gln Leu Phe Asn Glu Thr Leu 
945                 950                 955                 960 


Tyr Ser Gly Lys Tyr Asp Lys Gly Lys Asn Thr Ile Lys Lys Val Glu 
                965                 970                 975     


Lys Leu Asn Leu Leu Asp Asn Arg Thr Asp Lys Ile Lys Lys Ile Glu 
            980                 985                 990         


Glu Phe Phe Asp Glu Asp Lys Leu  Lys Glu Asn Glu Leu  Thr Lys Leu 
        995                 1000                 1005             


His Ile  Phe Asn His Asp Lys  Asn Leu Tyr Glu Thr  Leu Lys Ile 
    1010                 1015                 1020             


Ile Trp  Asn Glu Val Lys Ile  Glu Ile Lys Asn Lys  Asn Leu Asn 
    1025                 1030                 1035             


Glu Lys  Asn Tyr Phe Lys Tyr  Phe Val Asn Lys Lys  Leu Gln Glu 
    1040                 1045                 1050             


Gly Lys  Ile Ser Phe Asn Glu  Trp Val Pro Ile Leu  Asp Asn Asp 
    1055                 1060                 1065             


Phe Lys  Ile Ile Arg Lys Ile  Arg Tyr Ile Lys Phe  Ser Ser Glu 
    1070                 1075                 1080             


Glu Lys  Glu Thr Asp Glu Ile  Ile Phe Ser Gln Ser  Asn Phe Leu 
    1085                 1090                 1095             


Lys Ile  Asp Gln Arg Gln Asn  Phe Ser Phe His Asn  Thr Leu Tyr 
    1100                 1105                 1110             


Trp Val  Gln Ile Trp Val Tyr  Lys Asn Gln Lys Asp  Gln Tyr Cys 
    1115                 1120                 1125             


Phe Ile  Ser Ile Asp Ala Arg  Asn Ser Lys Phe Glu  Lys Asp Glu 
    1130                 1135                 1140             


Ile Lys  Ile Asn Tyr Glu Lys  Leu Lys Thr Gln Lys  Glu Lys Leu 
    1145                 1150                 1155             


Gln Ile  Ile Asn Glu Glu Pro  Ile Leu Lys Ile Asn  Lys Gly Asp 
    1160                 1165                 1170             


Leu Phe  Glu Asn Glu Glu Lys  Glu Leu Phe Tyr Ile  Val Gly Arg 
    1175                 1180                 1185             


Asp Glu  Lys Pro Gln Lys Leu  Glu Ile Lys Tyr Ile  Leu Gly Lys 
    1190                 1195                 1200             


Lys Ile  Lys Asp Gln Lys Gln  Ile Gln Lys Pro Val  Lys Lys Tyr 
    1205                 1210                 1215             


Phe Pro  Asn Trp Lys Lys Val  Asn Leu Thr Tyr Met  Gly Glu Ile 
    1220                 1225                 1230             


Phe Lys  Lys 
    1235     


<210>  788
<211>  1238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  788

Met Thr Lys Val Leu Gly Leu Asp Leu Gly Ala Ala Ser Val Gly Trp 
1               5                   10                  15      


Ala Met Ile Glu Glu Glu Glu Lys Ser His Lys Ile Leu Gly Leu Gly 
            20                  25                  30          


Ser Arg Ile Ile Pro Tyr Glu Gly Thr Glu Gly Asn Asp Phe Ala Lys 
        35                  40                  45              


Gly Thr Gly Glu Ser Arg Asn Ala Leu Arg Thr Lys Ala Arg Thr Ala 
    50                  55                  60                  


Arg Lys Gly Tyr Asp Arg Tyr Gln Leu Arg Arg Lys Tyr Leu Val Asp 
65                  70                  75                  80  


Thr Leu Val Lys Asn Glu Met Met Pro Asp Glu Tyr Leu Arg Lys Leu 
                85                  90                  95      


Pro Lys Met Gln Leu Trp Glu Leu Arg Ser Lys Ala Val Thr Thr Glu 
            100                 105                 110         


Ile Thr Lys Gln Glu Leu Gly Arg Leu Phe Leu Trp Leu Asn Gln Lys 
        115                 120                 125             


Arg Gly Tyr Lys Ser Ser Arg Ser Asp Ala Asn Leu Asp Lys Lys Asp 
    130                 135                 140                 


Thr Glu Tyr Val Val Glu Val Lys Ser Arg His Glu Lys Ile Lys Glu 
145                 150                 155                 160 


Ser Gly Phe Thr Ile Gly Gln Tyr Phe Tyr Ser Glu Leu Lys Ser Asn 
                165                 170                 175     


Glu Tyr Phe Arg Val Lys Glu Asn Ile Phe Pro Arg Glu Ala Tyr Ile 
            180                 185                 190         


Glu Glu Phe Asp Ala Ile Cys Lys Gln Gln Lys Arg His Leu Thr Asp 
        195                 200                 205             


Lys Leu Ile Ser Arg Ile Arg Asn Glu Ile Ile Tyr Tyr Gln Arg Pro 
    210                 215                 220                 


Leu Lys Ser Gln Lys Gly Leu Val Ser Val Cys Arg Phe Glu Gly Phe 
225                 230                 235                 240 


Phe Lys Lys Gly Ile Asn Gly Lys Glu Tyr Phe Val Gly Pro Lys Val 
                245                 250                 255     


Ala Pro Lys Ser Ser Pro Leu Phe Gln Thr Val Lys Ile Trp Glu Asn 
            260                 265                 270         


Ile Asn Ser Ile Arg Leu Ser Asp Arg Asn Gly Lys Glu Ile Met Leu 
        275                 280                 285             


Ser Leu Glu Gln Lys Lys Cys Ile Phe Asn His Leu Asp Asn Asn Glu 
    290                 295                 300                 


Lys Leu Thr Pro Lys Asp Leu Phe Ile Ile Ile Gly Val Lys Lys Glu 
305                 310                 315                 320 


Asn Cys Phe Val Asn Lys Gln Leu Glu Lys Gly Ile Gln Gly Asn Val 
                325                 330                 335     


Thr Lys Ser Thr Ile Leu Lys Cys Phe Gly Glu Asn Asn Asp Tyr Asp 
            340                 345                 350         


Lys Trp Leu Lys Leu Asp Leu Asn Ile Ile Val Lys Lys Asp Thr Phe 
        355                 360                 365             


Gly Tyr Leu Tyr Asp Lys Lys Thr Gly Glu Ile Ile Gly Glu Lys Leu 
    370                 375                 380                 


Ile Lys His Val Asp Ser Ala Val Glu Arg Glu Pro Leu Tyr Gln Leu 
385                 390                 395                 400 


Trp His Thr Ile Tyr Ser Ile Asn Asn Thr Asp Glu Cys Ser Ala Ala 
                405                 410                 415     


Leu Gln Asn Lys Phe Gly Phe Asp Ser Glu Thr Ala Asp Lys Leu Ser 
            420                 425                 430         


Asn Ile Asp Phe Gly Lys Ala Gly Phe Gly Asn Lys Ser Ala Lys Val 
        435                 440                 445             


Ile Arg Lys Ile Leu Pro Tyr Leu Met Glu Gly Asp Asp Tyr Ser Gln 
    450                 455                 460                 


Ser Met Ser Tyr Ala Gly Phe Asn His Ser Asp Ser Leu Thr Lys Asp 
465                 470                 475                 480 


Glu Asn Leu Lys Arg Lys Leu Leu Asp Lys Leu Arg Pro Ile Glu Lys 
                485                 490                 495     


Asn Ser Leu Arg Gln Pro Ile Val Glu Lys Ile Leu Asn Gln Met Val 
            500                 505                 510         


Asn Val Val Asn Ala Ile Ile Asp Lys Tyr Gly Lys Pro Asp Glu Ile 
        515                 520                 525             


Arg Ile Glu Leu Ala Arg Glu Leu Lys Gln Asn Lys Glu Glu Arg Asn 
    530                 535                 540                 


Lys Thr Asp Lys Asp Met Ser Lys Arg Gln Arg Glu Asn Gly Glu Ile 
545                 550                 555                 560 


Ser Lys Arg Leu Ala Glu Tyr Gly Leu Arg Ala Thr Arg Asn Asn Ile 
                565                 570                 575     


Ile Lys Trp Arg Leu Tyr Glu Glu Ile Asp Asn Gln Asp Lys Lys Leu 
            580                 585                 590         


Asn Ala Ile Cys Val Tyr Cys Gly Gln Pro Ile Ser Leu Thr Glu Ala 
        595                 600                 605             


Ile Lys Gly Asn Glu Val Asp Val Glu His Ile Ile Pro Lys Ser Lys 
    610                 615                 620                 


Leu Phe Asp Asp Ser Gln Ser Asn Lys Thr Leu Ala His Arg Arg Cys 
625                 630                 635                 640 


Asn Ser Thr Lys Gly Asp Met Thr Ala Tyr Asp Phe Met Glu Asn Lys 
                645                 650                 655     


Pro Ala Gln Val Phe Asn Ala Tyr Ile Glu Arg Val Asn Met Leu Tyr 
            660                 665                 670         


Asp Asn Lys Ile Ile Arg Lys Val Lys Arg Asp Lys Leu Leu Met Pro 
        675                 680                 685             


Glu Ser Lys Ile Pro Asp Asn Phe Ile Asp Arg Gln Leu Arg Glu Ser 
    690                 695                 700                 


Gln Tyr Ile Ala Ser Lys Ala Arg Glu Ile Leu Gln Thr Ile Cys Tyr 
705                 710                 715                 720 


Asn Val Trp Ser Thr Ser Gly Thr Val Thr Ala Arg Leu Arg His Leu 
                725                 730                 735     


Trp Gly Trp Asp Asp Ala Thr Met Ser Leu Gln Leu Pro Lys Tyr Arg 
            740                 745                 750         


Glu Leu Gly Leu Thr Glu Met Val Glu Trp Glu Ser Glu His Gly Lys 
        755                 760                 765             


Gln Lys His Ser Lys Glu Val Ile Ser Asp Trp Ser Lys Arg Asp Asp 
    770                 775                 780                 


His Arg His His Ala Val Asp Ala Leu Thr Ile Ala Cys Thr Lys Gln 
785                 790                 795                 800 


Gly Phe Ile Gln Arg Phe Asn Thr Leu Asn Ser Ser Lys Thr Lys Glu 
                805                 810                 815     


Asp Met Glu Phe Glu Ile Ala Lys Cys Ser Ile Glu Tyr Arg Glu Asn 
            820                 825                 830         


Leu Ser Lys Leu Glu Lys Tyr Ile Ile Ala Leu Arg Pro Ile Pro Val 
        835                 840                 845             


Ser Glu Val Glu Asp Ala Val Ala Asn Ile Leu Ile Ser Phe Lys Ser 
    850                 855                 860                 


Gly Lys Lys Val Ala Val Lys Gly Lys Arg Lys Ile Gly Lys Arg Gly 
865                 870                 875                 880 


Asn Lys Lys Ile Ile Gln Thr Asp Ile Ile Ile Pro His Gly Ser Leu 
                885                 890                 895     


Ser Glu Glu Ser Val Tyr Gly Lys Ile Lys Thr Ile Glu Arg Glu Lys 
            900                 905                 910         


Pro Leu Lys Tyr Leu Phe Gln Asn Ser Gln Leu Ile Phe Asn Asn Lys 
        915                 920                 925             


Ile Lys Ile Leu Val Glu Glu Arg Leu Ala Thr Phe Glu Asn Asp Thr 
    930                 935                 940                 


Lys Lys Ala Leu Ala Ser Leu Lys Lys Val Pro Val Tyr Leu Asp Glu 
945                 950                 955                 960 


Glu Lys Thr Ile Val Leu Glu Tyr Ala Thr Cys Phe Lys Glu Glu Tyr 
                965                 970                 975     


Val Lys Lys Tyr Asp Val Asp Thr Asn Phe Ser Lys Thr Asp Lys Val 
            980                 985                 990         


Ile Asp Asp Lys Ile Lys Glu Ile  Leu Gln His Arg Leu  Gly Lys Phe 
        995                 1000                 1005             


Gly Gly  Lys Ala Lys Glu Lys  Glu Ala Phe Lys Asp  Val Gln Gln 
    1010                 1015                 1020             


Gly Asp  Lys Thr Ile Lys Trp  Tyr Glu Asp Glu Gly  Leu Glu Arg 
    1025                 1030                 1035             


Pro Ile  His Ser Val Arg Cys  Phe Thr Gly Leu Ser  Ala Ile Val 
    1040                 1045                 1050             


Pro Val  Lys Lys Asp Glu Asn  Gly Ile Asp Ile Gly  Phe Val Lys 
    1055                 1060                 1065             


Pro Gly  Asn Asn His His Ile  Ala Ile Tyr Ser Asp  Lys Asp Gly 
    1070                 1075                 1080             


Asn Arg  Tyr Glu His Ile Cys  Thr Phe Trp His Ala  Val Glu Arg 
    1085                 1090                 1095             


Lys Lys  Tyr Asp Ile Pro Val  Val Ile Lys Asp Ser  Asn Glu Ile 
    1100                 1105                 1110             


Trp Asn  Lys Ile Trp Ser Lys  Pro Glu Gly Ser Tyr  Pro Gln Ser 
    1115                 1120                 1125             


Phe Leu  Asp Lys Leu Pro Gln  Glu Asn Leu Thr Leu  Asp Leu Ser 
    1130                 1135                 1140             


Met Gln  Gln Asn Glu Met Leu  Ile Leu Gly Met Ser  Tyr Glu Asp 
    1145                 1150                 1155             


Ile Lys  Thr Ala Ile Asp Asn  Ser Asp Tyr Lys Gln  Leu Ser Ser 
    1160                 1165                 1170             


Asn Leu  Tyr Arg Leu Gln Lys  Leu Ala Asn Ala Asn  Tyr Met Phe 
    1175                 1180                 1185             


Arg His  His Leu Glu Thr Gln  Ile Ile Asp Asp Asn  Asn Ser Lys 
    1190                 1195                 1200             


Ala Ser  Arg Arg Phe Ile Asn  Ile His Ser Leu Lys  Ala Leu Phe 
    1205                 1210                 1215             


Ala Trp  Asn Pro Tyr Lys Val  Asn Ile Asn Cys Leu  Gly Asp Ile 
    1220                 1225                 1230             


Gln Thr  Asn Ser Arg 
    1235             


<210>  789
<211>  1239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  789

Met Thr Glu Lys Arg Lys Phe Asp Ile Arg Glu Val Tyr Leu Ala Ile 
1               5                   10                  15      


His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn Ser Ala Thr Val 
            20                  25                  30          


Asp Ser Phe Lys Thr Ser Asn Ile Asp Phe Thr Ser Gln Phe Asp Arg 
        35                  40                  45              


Leu Asn Glu Leu Tyr Arg Gln Val Ile Leu Glu Glu Pro Phe Gln Ile 
    50                  55                  60                  


Asn Met Asn Gln Val Asp Lys Met Thr Asn Lys Leu Leu Asp Asn Asp 
65                  70                  75                  80  


Ala Leu Lys Leu Asp Thr Gln Lys Gln Val Ala Lys Leu Leu Pro Val 
                85                  90                  95      


Ile Tyr Asn Asp Lys Pro Val Asp Lys Gln Tyr Thr Arg Leu Ala Thr 
            100                 105                 110         


Glu Phe Ser Lys Ala Ile Leu Gly Tyr Lys Thr Lys Leu Asp Val Ile 
        115                 120                 125             


Leu Asn Leu Asp Thr Gln Asn Ala Lys Asp Trp Ala Ile Arg Leu Asp 
    130                 135                 140                 


Asp Glu Asp Ile Asp Asp Lys Leu Pro Ala Leu Val Glu Asn Leu Asp 
145                 150                 155                 160 


Glu Ser Arg Gln Glu Ile Val Thr Ile Ile Arg Asp Leu Tyr Ala Gln 
                165                 170                 175     


Ile Thr Leu Asn Ala Ile Val Pro Lys Gly Lys Ser Leu Ser Glu Ser 
            180                 185                 190         


Met Val Asp Lys Tyr Asn Asp His Lys Asp His Leu Asp Leu Leu Met 
        195                 200                 205             


Gly Leu Ile His Glu Leu Gly Asp Asp Ser Ala Lys Gly Ile Lys Leu 
    210                 215                 220                 


Arg Glu Ala Tyr Thr Gln Tyr Val Gly Lys Ser Gly Asp Lys Thr Leu 
225                 230                 235                 240 


Asn Gln Asp Asp Phe Tyr Ala Ala Ile Lys Lys Asn Leu Asp Glu Asp 
                245                 250                 255     


Ser Lys Arg Ser Pro Lys Ile Gln Arg Leu Ile Glu Gln Ala Ser Phe 
            260                 265                 270         


Met Pro Lys Gln Arg Thr Ser Ala Asn Gly Val Ile Pro His Gln Leu 
        275                 280                 285             


His Gln Leu Glu Leu Asp Arg Ile Ile Glu Asn Gln Gly Arg Tyr Tyr 
    290                 295                 300                 


Pro Phe Leu Lys Glu Pro Asn Ser Asn Thr His Lys Pro Asn Gly Gly 
305                 310                 315                 320 


Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Ile Pro Tyr Tyr Val 
                325                 330                 335     


Gly Pro Leu Ile Thr Lys Gln Asp Gln Glu Lys Thr Ser Gly Ala Asp 
            340                 345                 350         


Phe Ala Trp Met Ile Arg Lys Pro Gly Gln Gly Gly Glu Ile Thr Pro 
        355                 360                 365             


Trp Asn Phe Asp Gln Glu Val Asp Arg Met Ala Ser Ala Asn Thr Phe 
    370                 375                 380                 


Ile Arg Arg Met Thr Thr Lys Asp Ser Tyr Leu Val Ala Glu Asp Val 
385                 390                 395                 400 


Leu Pro Asp Ser Ser Leu Ile Tyr Glu Lys Phe Lys Val Leu Asn Glu 
                405                 410                 415     


Leu Asn Met Leu Lys Val Asn Asp Arg Arg Leu Ser Ile Ser Gln Lys 
            420                 425                 430         


Gln Asp Leu Phe Asn Asn Leu Phe Lys Lys Gln Lys Thr Ile Arg Thr 
        435                 440                 445             


Lys Lys Leu Gln Thr Tyr Ile Arg Thr Lys Trp Glu Leu Pro Ser Val 
    450                 455                 460                 


Met Ile Ser Gly Leu Ser Asp Pro Asp Lys Phe Asn Ser Ser Leu Ala 
465                 470                 475                 480 


Thr Tyr Ile Asp Phe Arg Asp Val Phe Gly Glu Lys Val Asp Asp Pro 
                485                 490                 495     


Asn Arg Gln Ala Asp Tyr Glu Lys Ile Ile Glu Trp Ser Thr Val Phe 
            500                 505                 510         


Glu Asp Arg Lys Ile Tyr Gln Ala Lys Leu Asn Gln Leu Asp Trp Leu 
        515                 520                 525             


Thr Glu Ser Gln Ser Lys Ala Leu Leu Thr Lys His Tyr Thr Gly Trp 
    530                 535                 540                 


Gly Arg Leu Ser Lys Lys Leu Leu Thr Gly Leu Lys Asp Gln Asn Gly 
545                 550                 555                 560 


Asp Ser Ile Leu Asp Gln Leu Trp Lys Thr Asn Asp Asn Phe Met Gln 
                565                 570                 575     


Ile Gln Ser Arg Asp Glu Phe Ala Lys Gln Ile His Asp Glu Asn Ala 
            580                 585                 590         


Lys Gln Phe His Glu Ala Asn Asn Val Asp Asp Ile Leu Asp Asp Ala 
        595                 600                 605             


Phe Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Asp Lys Val 
    610                 615                 620                 


Val Gln Asp Val Val Lys Ala Val Gly Tyr Ala Pro Asp Lys Ile Ala 
625                 630                 635                 640 


Ile Glu Phe Thr Arg Ser Pro Gln Asp Arg Pro Gln Arg Thr Phe Ser 
                645                 650                 655     


Arg Gln Arg Gln Leu Gln Ser Thr Tyr Leu Asn Val Ala Lys Ala Leu 
            660                 665                 670         


Met Lys Ser Asn Leu Asn Gln Glu Leu Lys Ser Val Ile Asp Ser Gln 
        675                 680                 685             


Gln Thr Leu Thr Asp Lys Leu Tyr Leu Tyr Phe Thr Gln Leu Gly Gln 
    690                 695                 700                 


Asp Met Tyr Thr Gly Lys Glu Ile Asn Phe Asp Glu Leu Val Asn Tyr 
705                 710                 715                 720 


Gln Ile Asp His Ile Leu Pro Gln Ala Phe Ile Lys Asp Asp Ser Leu 
                725                 730                 735     


Asp Asn Arg Val Leu Val Ser Ala Pro Ile Asn Asn Ala Lys Ser Asp 
            740                 745                 750         


Asn Val Pro Val Lys Lys Phe Gly Ala Lys Met Gly Tyr Phe Trp Lys 
        755                 760                 765             


Gln Leu Ala Glu Asn His Leu Ile Ser Lys Arg Lys Leu Asn Asn Leu 
    770                 775                 780                 


Thr Thr Asp Pro Asp Lys Ile Gly Lys Phe Thr Ala Gln Gly Phe Ile 
785                 790                 795                 800 


His Arg Gln Leu Val Glu Thr Ser Gln Val Ile Arg Leu Val Ala Asn 
                805                 810                 815     


Ile Leu Gly Asn Glu Tyr Gly His Asp Gly Thr Thr Ile Ile Glu Val 
            820                 825                 830         


Thr Ala Lys Met Asn His Gln Met Arg Lys Asp Phe Asp Leu Ile Lys 
        835                 840                 845             


Ile Arg Asp Val Asn Asp Tyr His His Ala Met Asp Ala Tyr Leu Thr 
    850                 855                 860                 


Ala Tyr Val Gly Asp Tyr Leu Tyr Leu Arg Tyr Pro Lys Leu Arg Ser 
865                 870                 875                 880 


Tyr Phe Val Tyr Gly Asp Phe Lys Lys Leu Lys Asp Gln Ser Leu Lys 
                885                 890                 895     


Ile Arg Asn Phe Asn Phe Leu His Asp Leu Thr Asn Asp Glu Ala Gly 
            900                 905                 910         


Asp Lys Ile Val Asp Gln Glu Thr Gly Glu Ile Ile Trp Asp Lys His 
        915                 920                 925             


Glu Ser Val Lys Gln Leu Lys Lys Val Tyr His Tyr Lys Phe Met Leu 
    930                 935                 940                 


Val Ser Gln Glu Val Tyr Thr Arg Gln Asp Ala Leu Phe Asn Gln Thr 
945                 950                 955                 960 


Ile Tyr Pro Ala Ser Asp Ala Asp Lys Arg Lys Leu Ile Pro Ile Lys 
                965                 970                 975     


Asn Asn Lys Pro Val Asp Val Tyr Gly Gly Tyr Ser Gly Asn Val Asp 
            980                 985                 990         


Ala Tyr Met Ala Ile Val Arg Ile  His Gly Lys Lys Glu  Asp Lys Tyr 
        995                 1000                 1005             


Lys Val  Val Gly Val Pro Met  Arg Ala Val Ala Asp  Leu Lys Lys 
    1010                 1015                 1020             


Ala Glu  Ser Lys Gly Arg Asp  Asn Tyr Leu Asn Ala  Val His Glu 
    1025                 1030                 1035             


Val Leu  Lys Pro Gln Phe Thr  Lys Lys Lys Lys Asp  Arg Lys Thr 
    1040                 1045                 1050             


Gly Glu  Ile Thr Glu Met Met  Asp Asp Phe Asp Val  Leu Leu Gly 
    1055                 1060                 1065             


Lys Val  Tyr Tyr Arg Gln Leu  Ile Val Asp Gly Asn  Lys Lys Phe 
    1070                 1075                 1080             


Met Leu  Gly Ser Ser Thr Tyr  Gln Tyr Asn Ala Lys  Gln Leu Val 
    1085                 1090                 1095             


Leu Ser  Asp Lys Ala Met Gln  Val Leu Ser Lys Asp  Asp Lys Leu 
    1100                 1105                 1110             


Lys Asn  Gln Asp Glu Asn Gln  Asn Leu Ile Asp Val  Tyr Asp Glu 
    1115                 1120                 1125             


Ile Leu  Glu Lys Val Asp Gln  Tyr Phe Glu Leu Tyr  Asp Ile Asn 
    1130                 1135                 1140             


Lys Phe  Arg Gln Lys Leu His  Asp Gly Arg Lys Lys  Phe Ile Glu 
    1145                 1150                 1155             


Leu Pro  Val Asp Asn Asp Phe  Asn Gly Lys Lys Leu  Ile Ser Tyr 
    1160                 1165                 1170             


Gly Lys  Arg Ala Thr Ile Ile  Ser Ile Leu Asn Gly  Leu His Ala 
    1175                 1180                 1185             


Asn Ala  Thr Met Ser Asn Leu  Lys Tyr Leu Gly Ile  Ser Ser Pro 
    1190                 1195                 1200             


Phe Gly  Met Leu Gln Val Pro  Asn Gly Ile Ile Leu  Ser Pro Ala 
    1205                 1210                 1215             


Ser Arg  Ile Cys Tyr Gln Ser  Pro Thr Gly Leu Phe  Glu Arg Lys 
    1220                 1225                 1230             


Val Lys  Leu Ser Asp Leu 
    1235                 


<210>  790
<211>  1245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  790

Met Ile Ile Tyr Phe Ile Phe Glu Lys Ile Val Asn Thr Ile Leu Val 
1               5                   10                  15      


Met Lys Lys Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
            20                  25                  30          


Ala Leu Ile Asn Ala Tyr Ile Asp Glu Tyr Asn Lys Glu His Leu Tyr 
        35                  40                  45              


Gly Ile Glu Ala Cys Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile 
    50                  55                  60                  


Leu Gly Asn Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ala Asp Arg 
65                  70                  75                  80  


Thr Ser Tyr Arg Gly Ile Arg Arg Leu Arg Glu Arg His Leu Leu Arg 
                85                  90                  95      


Arg Glu Arg Leu His Arg Ile Leu Asp Leu Leu Gly Phe Leu Pro Lys 
            100                 105                 110         


His Tyr Ser Asp Ser Leu Asn Arg Tyr Gly Lys Phe Leu Asn Asp Ile 
        115                 120                 125             


Glu Cys Lys Leu Pro Trp Val Lys Asp Glu Thr Gly Ser Tyr Lys Phe 
    130                 135                 140                 


Ile Phe Gln Glu Ser Phe Lys Glu Met Leu Ala Asp Phe Thr Glu His 
145                 150                 155                 160 


His Pro Lys Leu Ile Ala Asn Asn Lys Lys Val Pro Tyr Asp Trp Thr 
                165                 170                 175     


Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Lys Ile Ser Lys Glu 
            180                 185                 190         


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
        195                 200                 205             


Gln Leu Arg Gly Glu Glu Glu Glu Thr Pro Asn Lys Leu Val Glu Tyr 
    210                 215                 220                 


Tyr Ser Leu Lys Val Glu Lys Val Glu Asp Ser Gly Glu Arg Lys Gly 
225                 230                 235                 240 


Lys Asp Thr Trp Tyr Asn Val His Leu Glu Asn Gly Met Ile Tyr Arg 
                245                 250                 255     


Arg Thr Ser Asn Ile Pro Leu Asp Trp Glu Gly Lys Thr Lys Glu Phe 
            260                 265                 270         


Ile Val Thr Thr Asp Leu Glu Ala Asp Gly Ser Pro Lys Lys Asp Lys 
        275                 280                 285             


Glu Gly Asn Ile Lys Arg Ser Phe Arg Ala Pro Lys Asp Asp Asp Trp 
    290                 295                 300                 


Thr Leu Ile Lys Lys Lys Thr Glu Ala Asp Ile Asp Lys Ile Lys Met 
305                 310                 315                 320 


Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Gln Lys Pro Asp Gln 
                325                 330                 335     


Lys Ile Arg Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr Lys 
            340                 345                 350         


Asn Glu Leu Tyr Gln Ile Leu Lys Thr Gln Ser Glu Phe His Glu Glu 
        355                 360                 365             


Leu Arg Asp Lys Gln Leu Tyr Ile Ala Cys Leu Asn Glu Leu Tyr Pro 
    370                 375                 380                 


Asn Asn Glu Pro Arg Arg Asn Ser Ile Ser Thr Arg Asp Phe Cys His 
385                 390                 395                 400 


Leu Phe Ile Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
                405                 410                 415     


Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu Asn Arg Tyr Ile Asp 
            420                 425                 430         


Lys Glu Ser Gly Glu Ile Lys His Ala Ser Ile Lys Cys Ile Ala Lys 
        435                 440                 445             


Ser His Pro Leu Tyr Gln Glu Phe Arg Leu Trp Gln Phe Ile Val Asn 
    450                 455                 460                 


Leu Arg Ile Tyr Arg Lys Glu Thr Asp Val Asp Val Thr Gln Glu Leu 
465                 470                 475                 480 


Leu Pro Thr Glu Ala Asp Tyr Val Thr Leu Phe Glu Trp Leu Asn Glu 
                485                 490                 495     


Lys Lys Glu Ile Asp Gln Lys Ala Phe Phe Lys Tyr Pro Pro Phe Gly 
            500                 505                 510         


Phe Lys Lys Thr Thr Ser Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys 
        515                 520                 525             


Pro Tyr Pro Cys Asn Glu Thr His Ala Gln Ile Ile Thr Arg Leu Gly 
    530                 535                 540                 


Lys Ala His Ile Pro Lys Ala Phe Leu Ser Lys Glu Lys Glu Glu Thr 
545                 550                 555                 560 


Leu Trp His Ile Leu Tyr Ser Ile Glu Asp Lys Gln Glu Ile Glu Lys 
                565                 570                 575     


Ala Leu His Ser Phe Ala Asn Lys Asn Asn Leu Ser Glu Glu Phe Ile 
            580                 585                 590         


Glu Gln Phe Lys Asn Phe Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr 
        595                 600                 605             


Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Met Gly Lys 
    610                 615                 620                 


Tyr Trp Ser Ile Glu Asn Ile Asp Asn Gly Thr Arg Ile Arg Ile Asn 
625                 630                 635                 640 


Lys Ile Ile Asp Gly Glu Tyr Asp Glu Asn Ile Arg Glu Arg Val Arg 
                645                 650                 655     


Gln Lys Ala Ile Asn Leu Thr Asp Ile Thr His Phe Arg Ala Leu Pro 
            660                 665                 670         


Leu Trp Leu Ala Cys Tyr Leu Val Tyr Asp Arg His Ser Glu Val Lys 
        675                 680                 685             


Asp Ile Val Lys Trp Lys Thr Pro Lys Asp Ile Asp Leu Tyr Leu Lys 
    690                 695                 700                 


Ser Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile 
705                 710                 715                 720 


Thr Glu Thr Leu Arg Thr Val Arg Asp Ile Trp Gln Gln Val Gly His 
                725                 730                 735     


Ile Asp Glu Ile His Ile Glu Leu Gly Arg Glu Met Lys Asn Pro Ala 
            740                 745                 750         


Asp Lys Arg Ala Arg Met Ser Gln Gln Met Ile Lys Asn Glu Asn Thr 
        755                 760                 765             


Asn Leu Arg Ile Lys Ala Leu Leu Thr Glu Phe Leu Asn Pro Glu Phe 
    770                 775                 780                 


Gly Ile Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln Asp Leu Leu 
785                 790                 795                 800 


Arg Ile Tyr Glu Glu Gly Val Leu Asn Ser Ile Leu Glu Leu Pro Glu 
                805                 810                 815     


Asp Ile Gly Ile Ile Leu Gly Lys Phe Asn Gln Thr Asp Thr Leu Lys 
            820                 825                 830         


Arg Pro Thr Arg Ser Glu Ile Leu Arg Tyr Lys Leu Trp Leu Glu Gln 
        835                 840                 845             


Lys Tyr Arg Ser Pro Tyr Thr Gly Glu Met Ile Pro Leu Ser Lys Leu 
    850                 855                 860                 


Phe Thr Pro Ala Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr 
865                 870                 875                 880 


Phe Asp Asp Ser Leu Ser Asn Lys Val Ile Cys Glu Ser Glu Ile Asn 
                885                 890                 895     


Lys Leu Lys Asp Arg Ser Leu Gly Tyr Glu Phe Ile Lys Asn His His 
            900                 905                 910         


Gly Glu Lys Val Glu Leu Ala Phe Asp Lys Pro Val Glu Val Leu Ser 
        915                 920                 925             


Val Glu Ala Tyr Glu Lys Leu Val His Glu Ser Tyr Ser His Asn Arg 
    930                 935                 940                 


Ser Lys Met Lys Lys Leu Leu Met Glu Asp Ile Pro Asp Gln Phe Ile 
945                 950                 955                 960 


Glu Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser 
                965                 970                 975     


Leu Leu Ser Asn Ile Val Arg Glu Glu Asn Glu Gln Glu Ala Ile Ser 
            980                 985                 990         


Lys Asn Val Ile Pro Cys Thr Gly  Gly Ile Thr Asp Arg  Leu Lys Lys 
        995                 1000                 1005             


Asp Trp  Gly Ile Asn Asp Val  Trp Asn Lys Ile Val  Leu Pro Arg 
    1010                 1015                 1020             


Phe Ile  Arg Leu Asn Glu Leu  Thr Glu Ser Thr Arg  Phe Thr Ser 
    1025                 1030                 1035             


Ile Asn  Thr Asn Asn Thr Met  Ile Pro Ser Met Pro  Leu Glu Leu 
    1040                 1045                 1050             


Gln Lys  Gly Phe Asn Lys Lys  Arg Ile Asp His Arg  His His Ala 
    1055                 1060                 1065             


Met Asp  Ala Ile Ile Ile Ala  Cys Ala Asn Arg Asn  Ile Val Asn 
    1070                 1075                 1080             


Tyr Leu  Asn Asn Val Ser Ala  Ser Lys Asn Thr Lys  Ile Thr Arg 
    1085                 1090                 1095             


Arg Asp  Leu Gln Thr Leu Leu  Cys His Lys Asp Lys  Thr Asp Asn 
    1100                 1105                 1110             


Asn Gly  Asn Tyr Lys Trp Val  Ile Asp Lys Pro Trp  Glu Thr Phe 
    1115                 1120                 1125             


Thr Gln  Asp Thr Leu Thr Ala  Leu Gln Lys Ile Thr  Val Ser Phe 
    1130                 1135                 1140             


Lys Gln  Asn Leu Arg Val Ile  Asn Lys Thr Thr Asn  His Tyr Gln 
    1145                 1150                 1155             


His Tyr  Glu Asn Gly Lys Lys  Ile Val Ser Asn Gln  Ser Lys Gly 
    1160                 1165                 1170             


Asp Ser  Trp Ala Ile Arg Lys  Ser Met His Lys Glu  Thr Val His 
    1175                 1180                 1185             


Gly Glu  Val Asn Leu Arg Met  Ile Lys Thr Val Ser  Leu Asn Glu 
    1190                 1195                 1200             


Ala Leu  Lys Lys Pro Gln Ala  Ile Val Glu Met Asp  Leu Lys Lys 
    1205                 1210                 1215             


Lys Ile  Leu Ala Met Leu Glu  Leu Gly Tyr Asp Thr  Lys Arg Ile 
    1220                 1225                 1230             


Lys Asn  Tyr Phe Glu Glu Asn  Lys Asp Thr Trp Gln  
    1235                 1240                 1245 


<210>  791
<211>  1246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  791

Met Arg Leu Val Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly 
1               5                   10                  15      


His Phe Leu Tyr Glu Gly Asp Leu Lys Ala Glu Asn Thr Asp Val Gln 
            20                  25                  30          


Ala Leu Phe Lys Asp Phe Val Glu Glu Tyr Asp Lys Thr Ile Glu Glu 
        35                  40                  45              


Ser His Leu Ser Glu Ile Thr Val Asp Ala Leu Ser Ile Leu Thr Glu 
    50                  55                  60                  


Lys Val Ser Lys Ser Ser Arg Leu Glu Asn Leu Ile Ala His Tyr Pro 
65                  70                  75                  80  


Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu 
                85                  90                  95      


Gly Leu Gln Ala Asn Phe Lys Thr Asn Phe Gln Leu Ser Glu Asp Ala 
            100                 105                 110         


Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu Glu Asp Leu Glu Gly Leu 
        115                 120                 125             


Leu Gly Glu Val Gly Asp Glu Tyr Ala Asp Leu Phe Val Ser Ala Lys 
    130                 135                 140                 


Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly Ile Leu Thr Val Asp Asp 
145                 150                 155                 160 


Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser Met Val Lys Arg Tyr Lys 
                165                 170                 175     


Glu His Lys Glu Glu Leu Ala Ala Phe Lys Arg Phe Ile Lys Glu Lys 
            180                 185                 190         


Leu Pro Lys Lys Tyr Glu Glu Ile Phe Lys Asp Asp Thr Lys Asn Gly 
        195                 200                 205             


Tyr Ala Gly Tyr Val Gly Ala Asp Lys Lys Leu Arg Lys Arg Ser Gly 
    210                 215                 220                 


Lys Leu Ala Thr Glu Glu Glu Phe Tyr Lys Tyr Val Lys Gly Ile Leu 
225                 230                 235                 240 


Asn Lys Val Glu Gly Ala Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu 
                245                 250                 255     


Asp Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His 
            260                 265                 270         


Gln Ile His Leu Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu 
        275                 280                 285             


His Tyr Pro Phe Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu 
    290                 295                 300                 


Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser 
305                 310                 315                 320 


Arg Phe Ala Trp Ala Glu Tyr Lys Ala Asp Glu Lys Ile Thr Pro Trp 
                325                 330                 335     


Asn Phe Asp Asp Ile Leu Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile 
            340                 345                 350         


Thr Arg Met Thr Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu 
        355                 360                 365             


Pro Lys His Ser Pro Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu 
    370                 375                 380                 


Thr Lys Val Lys Tyr Val Asn Glu Gln Gly Glu Ala Lys Phe Phe Asp 
385                 390                 395                 400 


Thr Asn Met Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg 
                405                 410                 415     


Lys Val Thr Lys Asp Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu 
            420                 425                 430         


Glu Phe Arg Ile Val Asn Leu Thr Gly Leu Asp Lys Glu Asn Lys Ala 
        435                 440                 445             


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Arg Lys Ile Leu Asp 
    450                 455                 460                 


Lys Ser Phe Leu Asp Asp Lys Val Asn Glu Lys Ile Ile Glu Asp Ile 
465                 470                 475                 480 


Ile Gln Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Gln Arg 
                485                 490                 495     


Leu Gln Lys Tyr Ser Asp Ile Phe Thr Thr Gln Gln Leu Lys Glu Leu 
            500                 505                 510         


Glu Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile 
        515                 520                 525             


Asn Gly Ile Arg Asn Lys Glu Asn Lys Lys Thr Ile Leu Asp Tyr Leu 
    530                 535                 540                 


Ile Asp Asp Gly Tyr Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp 
545                 550                 555                 560 


Asp Ala Leu Ser Phe Lys Glu Glu Ile Ala Arg Ala Gln Ile Ile Gly 
                565                 570                 575     


Asp Val Asp Asp Ile Ala Asn Val Val His Asp Leu Pro Gly Ser Pro 
            580                 585                 590         


Ala Ile Lys Lys Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu 
        595                 600                 605             


Val Lys Val Met Gly His Asn Pro Thr Asn Ile Ile Ile Glu Met Ala 
    610                 615                 620                 


Arg Glu Asn Gln Thr Thr Asp Lys Gly Arg Arg Asn Ser Gln Gln Arg 
625                 630                 635                 640 


Leu Lys Leu Leu Gln Asp Ser Leu Lys Asn Leu Asp Asn Pro Val Asn 
                645                 650                 655     


Ile Lys Asn Val Glu Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu 
            660                 665                 670         


Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Thr Leu Asp 
        675                 680                 685             


Ile Asn Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala 
    690                 695                 700                 


Phe Ile Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Thr Arg Ser Asp 
705                 710                 715                 720 


Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Ile Glu Val Val His 
                725                 730                 735     


Glu Met Lys Ser Phe Trp Ser Lys Leu Leu Ser Val Lys Leu Ile Thr 
            740                 745                 750         


Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
        755                 760                 765             


Glu Asn Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg 
    770                 775                 780                 


Gln Ile Thr Lys His Val Ala Gln Val Leu Asp Ala Arg Phe Asn Ala 
785                 790                 795                 800 


Lys His Asp Glu Asn Lys Lys Val Ile Arg Asp Val Lys Ile Ile Thr 
                805                 810                 815     


Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Asp Phe Lys Phe Tyr 
            820                 825                 830         


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
        835                 840                 845             


Asn Ala Val Ile Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala 
    850                 855                 860                 


Ser Glu Phe Val Tyr Gly Glu Phe Lys Lys Tyr Asp Val Arg Lys Phe 
865                 870                 875                 880 


Ile Ala Lys Ser Asp Lys Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe 
                885                 890                 895     


Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Lys Glu Val Lys Phe Ala 
            900                 905                 910         


Asp Gly Thr Val Val Glu Arg Pro Asp Ile Glu Thr Ser Glu Asp Gly 
        915                 920                 925             


Glu Ile Ala Trp Asn Lys Gln Thr Asp Phe Lys Ile Val Arg Lys Val 
    930                 935                 940                 


Leu Ser Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr 
945                 950                 955                 960 


His Gly Leu Asp Arg Gly Lys Pro Arg Gly Leu Phe Asn Ala Asn Pro 
                965                 970                 975     


Ser Pro Lys Pro Lys Pro Asp Ser Ser Glu Asn Leu Val Gly Ile Lys 
            980                 985                 990         


Arg Asn Leu Asp Pro Lys Lys Tyr  Gly Gly Tyr Ala Gly  Ile Ser Asn 
        995                 1000                 1005             


Ser Tyr  Ala Val Leu Val Lys  Ala Ile Ile Glu Lys  Gly Val Lys 
    1010                 1015                 1020             


Lys Lys  Glu Thr Met Val Leu  Glu Phe Gln Gly Ile  Ser Ile Leu 
    1025                 1030                 1035             


Asp Arg  Ile Thr Phe Glu Lys  Asp Lys Arg Ala Phe  Leu Leu Gly 
    1040                 1045                 1050             


Lys Gly  Tyr Lys Asp Ile Lys  Lys Ile Ile Glu Leu  Pro Lys Tyr 
    1055                 1060                 1065             


Ser Leu  Phe Glu Leu Lys Asp  Gly Ser Arg Arg Met  Leu Ala Ser 
    1070                 1075                 1080             


Ile Leu  Ser Thr Asn Asn Lys  Arg Gly Glu Ile His  Lys Gly Asn 
    1085                 1090                 1095             


Glu Leu  Phe Val Pro Gln Lys  Phe Thr Thr Leu Leu  Tyr His Ala 
    1100                 1105                 1110             


Lys Arg  Ile Asn Asn Pro Ile  Asn Lys Asp His Ile  Glu Tyr Val 
    1115                 1120                 1125             


Lys Lys  His Arg Asp Asp Phe  Lys Glu Leu Leu Asn  Tyr Val Leu 
    1130                 1135                 1140             


Glu Phe  Asn Glu Lys Tyr Val  Gly Ala Thr Lys Asn  Gly Glu Arg 
    1145                 1150                 1155             


Leu Lys  Glu Ala Val Ala Asp  Phe Asp Ser Lys Ser  Asn Glu Glu 
    1160                 1165                 1170             


Ile Cys  Thr Ser Phe Leu Gly  Ala Val Asn Ser Lys  Asn Ala Gly 
    1175                 1180                 1185             


Leu Phe  Glu Leu Thr Ser Leu  Gly Ser Ala Ser Asp  Phe Glu Phe 
    1190                 1195                 1200             


Leu Gly  Val Lys Ile Pro Arg  Tyr Arg Asp Tyr Thr  Pro Ser Ser 
    1205                 1210                 1215             


Leu Leu  Lys Asp Ser Ile Leu  Ile His Gln Ser Ile  Thr Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu Gly Glu  Asp 
    1235                 1240                 1245     


<210>  792
<211>  1248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  792

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Ser Ala Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Ile Thr Glu Asn Val Glu Asn Gly Ile Thr Lys Cys Asp Ile 
            20                  25                  30          


Leu Gly Met Gly Ser Arg Ile Ile Pro Tyr Glu Gly Thr Glu Gly Lys 
        35                  40                  45              


Asp Phe Thr Lys Gly Thr Gly Glu Ser Arg Asn Ser Leu Arg Thr Lys 
    50                  55                  60                  


Ala Lys Thr Ala Arg Lys Gly Tyr Asp Arg Tyr Gln Leu Arg Arg Lys 
65                  70                  75                  80  


Tyr Leu Val Asp Thr Leu Ile Lys Asn Gln Met Tyr Pro Asp Glu Ala 
                85                  90                  95      


Thr Arg Asn Leu Pro Lys Met Lys Leu Trp Glu Leu Arg Asp Lys Ala 
            100                 105                 110         


Val His Ser Pro Val Ser Leu Met Glu Leu Gly Arg Leu Leu Leu Trp 
        115                 120                 125             


Leu Asn Gln Lys Arg Gly Tyr Lys Ser Ser Arg Ser Asp Ala Asn Leu 
    130                 135                 140                 


Gly Lys Lys Asp Thr Glu Tyr Val Ala Thr Val Lys Ser Arg His Glu 
145                 150                 155                 160 


Lys Met Lys Glu Leu Asp Val Thr Ile Gly Gln Tyr Phe Tyr Arg Glu 
                165                 170                 175     


Leu Ser Ala Asn Glu Phe Phe Lys Val Lys Glu Asn Ile Phe Pro Arg 
            180                 185                 190         


Glu Ala Tyr Ile Glu Glu Phe Asp Ala Ile Cys Lys Thr Gln Gln Ile 
        195                 200                 205             


Ala His Pro Met Ile Leu Arg Ser Glu Leu Ile Asp Lys Ile Arg Asn 
    210                 215                 220                 


Glu Val Ile Tyr Tyr Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Val 
225                 230                 235                 240 


Ser Val Cys Glu Phe Glu Gly Phe Trp Arg Ile Gly Lys Asp Gly Lys 
                245                 250                 255     


Glu Tyr Phe Val Gly Pro Lys Val Ala Pro Lys Ser Ser Pro Leu Phe 
            260                 265                 270         


Gln Leu Ala Lys Met Trp Glu Asn Ile Asn Asn Ile Lys Ile Gly Ser 
        275                 280                 285             


Arg Thr Gly Glu Asp Leu Lys Leu Thr Thr Glu Gln Lys Trp Ala Ile 
    290                 295                 300                 


Phe Gln His Leu Asp Asn Asn Glu Lys Leu Thr Pro Thr Asp Leu Leu 
305                 310                 315                 320 


Lys Ile Leu Asn Leu Lys Lys Glu Asn Cys Tyr Val Asn Lys Gln Met 
                325                 330                 335     


Gly Lys Gly Leu Asn Gly Asn Ile Thr Lys His Ala Ile Leu Gln Cys 
            340                 345                 350         


Leu Gly Gly Ser Asp Lys Tyr Glu Asn Leu Leu Lys Leu Glu Leu Lys 
        355                 360                 365             


Met Val Asp Gly Asp Lys Glu Gly Tyr Leu Tyr Asp Arg Lys Thr Gly 
    370                 375                 380                 


Glu Val Leu Asn Ser Lys Met Ile Lys Phe Ile Asp Pro Met Val Glu 
385                 390                 395                 400 


Gln Glu Pro Phe Tyr Gln Leu Trp His Ile Ile Tyr Ser Ile His Asp 
                405                 410                 415     


Gln Glu Glu Cys Ser Arg Val Leu Gln Ala Lys Phe Asp Ile Glu Gln 
            420                 425                 430         


Pro Ile Ala Asp Lys Leu Ala Ala Ile Asp Phe Ser Lys His Ala Phe 
        435                 440                 445             


Gly Ser Lys Ser Ala Lys Val Ile Arg Lys Ile Leu Pro Tyr Leu Met 
    450                 455                 460                 


Asp Gly Asp Gly Tyr Ser Glu Ala Met Ser Tyr Ala Gly Tyr Asp His 
465                 470                 475                 480 


Ser Asn Ser Gln Thr Lys Glu Glu Asn Leu Gln Arg Lys Leu Leu Asp 
                485                 490                 495     


Leu Leu Lys Pro Ile Pro Lys Asn Ser Leu Arg Gln Pro Ile Val Glu 
            500                 505                 510         


Lys Ile Leu Asn Gln Met Val Asn Val Val Asn Ala Ile Ile Glu Lys 
        515                 520                 525             


Tyr Gly Asn Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
    530                 535                 540                 


Gln Ser Lys Glu Glu Arg Asn Asp Ala Asp Lys Ala Met Ser Lys Arg 
545                 550                 555                 560 


Gln Arg Glu Asn Asp Ala Ile Thr Lys Arg Leu Glu Glu Phe Gly Leu 
                565                 570                 575     


Arg Ala Thr Arg Asn Asn Ile Ile Lys Trp Arg Leu Tyr Glu Glu Ile 
            580                 585                 590         


Asp Asn Gln Glu Lys Lys Leu Asn Ala Met Cys Val Tyr Cys Gly Gln 
        595                 600                 605             


Pro Ile Ser Leu Thr Glu Ala Leu Arg Gly Asn Asp Val Asp Ile Glu 
    610                 615                 620                 


His Ile Ile Pro Lys Ser Lys Leu Phe Asp Asp Ser Gln Ser Asn Lys 
625                 630                 635                 640 


Thr Leu Ala His Arg His Cys Asn Lys Asn Lys Asn Asp Leu Thr Ala 
                645                 650                 655     


Tyr Asp Phe Met Lys Gly Lys Pro Glu Ala Val Phe Asn Glu Tyr Val 
            660                 665                 670         


Glu Arg Val Asn Leu Leu Tyr Asp Asn His Ile Ile Arg Lys Ala Lys 
        675                 680                 685             


Arg Asp Lys Leu Leu Met Thr Glu Ser Lys Ile Pro Asp Asn Phe Ile 
    690                 695                 700                 


Asp Arg Gln Leu Arg Glu Ser Gln Tyr Ile Ala Lys Lys Ala Arg Glu 
705                 710                 715                 720 


Ile Leu Gln Thr Ile Cys His Asp Val Trp Ser Thr Ser Gly Thr Val 
                725                 730                 735     


Thr Ala Glu Leu Arg His Leu Trp Gly Trp Asp Asp Val Thr Met Asn 
            740                 745                 750         


Leu Gln Met Ala Lys Tyr Arg Glu Leu Gly Leu Thr His Gln Val Glu 
        755                 760                 765             


Trp Glu Ser Glu His Gly Lys Asn Lys His Glu Lys Glu Val Ile Thr 
    770                 775                 780                 


Asp Trp Thr Lys Arg Asp Asp His Arg His His Ala Val Asp Ala Leu 
785                 790                 795                 800 


Thr Ile Ala Cys Thr Lys Gln Gly Phe Ile Gln Arg Phe Asn Thr Leu 
                805                 810                 815     


Ser Ala Ala Lys Thr Arg Glu Asp Met Met Phe Asp Ile Glu Gln Arg 
            820                 825                 830         


Ser His Glu Phe Lys Glu Lys Arg Ser Leu Leu Glu Lys Tyr Ile Ile 
        835                 840                 845             


Ser Glu Gln Pro Leu Lys Val Asp Glu Val Glu Lys Ala Val Ser Asn 
    850                 855                 860                 


Ile Leu Ile Ser Phe Lys Ser Gly Lys Lys Val Ala Val Trp Gly Thr 
865                 870                 875                 880 


Arg Lys Ile Gly Lys Arg Gly Ser Lys Lys Val Ile Gln Lys Lys Ile 
                885                 890                 895     


Ile Val Pro Arg Gly Ser Leu Ser Glu Glu Ser Val Tyr Gly Lys Ile 
            900                 905                 910         


Lys Lys Gln Glu Glu Lys Asp Tyr Lys Tyr Leu Phe Glu Asn Pro Gln 
        915                 920                 925             


Leu Ile Phe Asn Pro Thr Ile Lys Ala Lys Val Glu Glu Arg Leu Leu 
    930                 935                 940                 


Met Phe Asp Gly Asp Val Lys Lys Ala Ile Thr Ser Leu Lys Lys Ile 
945                 950                 955                 960 


Pro Ile Tyr Leu Asp Asn Asn Lys Thr Val Val Leu Glu Lys Ala Ile 
                965                 970                 975     


Cys Leu Lys Asp Glu Phe Val Ile Lys Tyr Ser Val Asp Ile Asn Phe 
            980                 985                 990         


Asn Lys Val Asp Lys Val Val Asp  Pro Lys Ile Lys Glu  Ile Leu Gln 
        995                 1000                 1005             


Asn Arg  Leu Lys Lys Phe Gly  Gly Lys Glu Lys Glu  Ala Phe Lys 
    1010                 1015                 1020             


Asp Val  Gln Leu Glu Asp Lys  Met Ile Ile Lys Trp  Tyr Glu Asp 
    1025                 1030                 1035             


Glu Gly  Leu Glu Arg Pro Ile  Lys Ser Val Arg Cys  Phe Thr Gly 
    1040                 1045                 1050             


Leu Ser  Ala Val Val Pro Val  Lys Lys Asp Glu Asn  Gly Asn Asp 
    1055                 1060                 1065             


Ile Gly  Phe Val Lys Pro Gly  Asn Asn His His Ile  Ala Ile Tyr 
    1070                 1075                 1080             


Thr Asp  Thr Glu Glu Asn Lys  Gln Gln His Val Cys  Thr Phe Trp 
    1085                 1090                 1095             


His Ala  Val Glu Arg Lys Lys  Tyr Asp Ile Pro Val  Ile Ile Lys 
    1100                 1105                 1110             


Asn Thr  Asn Glu Val Trp Asp  Lys Ile Gln Ser Gln  Pro Asp Gly 
    1115                 1120                 1125             


Thr Tyr  Pro Asp Ser Phe Leu  Glu Met Leu Pro Pro  Pro Asn Leu 
    1130                 1135                 1140             


Asn Leu  Leu Val Asn Met Gln  Gln Asn Glu Met Phe  Ile Leu Gly 
    1145                 1150                 1155             


Thr Thr  Thr Glu Asp Ile Lys  Leu Ala Ile Asp Lys  Arg Asp Tyr 
    1160                 1165                 1170             


Lys Ile  Ile Gly Asp Lys Leu  Tyr Arg Leu Gln Lys  Met Ser Ile 
    1175                 1180                 1185             


Lys Pro  Ser Ser Gly Gln Ile  Asp Ile Val Phe Arg  His His Leu 
    1190                 1195                 1200             


Glu Thr  Gln Leu Ile Asp Asp  Glu Asn Ser Lys Lys  Ser Lys Arg 
    1205                 1210                 1215             


Phe Tyr  Asn Val Gln Ser Leu  Gly Ala Leu Phe Ala  Leu Asn Pro 
    1220                 1225                 1230             


Phe Lys  Val Arg Ile Asp Asn  Leu Gly Asn Leu Val  Lys Glu Lys 
    1235                 1240                 1245             


<210>  793
<211>  1253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  793

Met Lys Ala Arg Gly Asn Phe Leu His Gln Asp Thr Pro Gly Leu Thr 
1               5                   10                  15      


Ala Ala Asn Ala Asn Met Gly Asp Ala Val Glu Lys Leu Cys Val Ala 
            20                  25                  30          


Leu Glu Asp Trp Cys Thr Leu Arg Asn Leu Glu Cys Asn Ile Arg Lys 
        35                  40                  45              


Asn Gln Lys Asn Ile Gln Asn Leu Leu Glu Gly Lys Thr Val Glu Val 
    50                  55                  60                  


Ser Ala Glu Asn Ala Leu Lys Glu Ser Phe Asp Ser Ser Arg Ala Ser 
65                  70                  75                  80  


Arg Lys Asp Lys Ala Thr Ile Leu Ser Thr Trp Ile Ala Ile Gln Pro 
                85                  90                  95      


Gly Glu Tyr Leu Asp Ala Lys Gln Ala Lys Asp Ala Ser Lys Glu Ile 
            100                 105                 110         


Ala Asn Val Leu Phe Gly Leu Gln Gly Asn Met Lys Lys Ile Phe Ile 
        115                 120                 125             


Glu Phe Glu Glu Asp Gln Lys Ile Arg Leu Asp Asn Asp Glu Gln Val 
    130                 135                 140                 


Glu Ala Phe Tyr Ser Leu Cys Pro Asp Asp Gly Val Ala Leu Phe Glu 
145                 150                 155                 160 


Ser Phe Gln Ser Ile Tyr Ser Ala Tyr Ile Leu Met Gly Ile Leu Lys 
                165                 170                 175     


Asn Ala Asn Gly Gly Ser Ile Ser Ser Cys Lys Val Ala Ala Tyr Asp 
            180                 185                 190         


Gln Tyr Lys Glu Asp Leu Lys Leu Leu Lys Asp Leu Val Arg Glu Phe 
        195                 200                 205             


Ala Ser Glu Lys Tyr Asp Glu Phe Phe Arg Gly Asp Ile Phe Asp Ser 
    210                 215                 220                 


Ser Lys Gly Phe Arg Ala Arg Asn Tyr Asn Pro Ala Thr Ala Lys Gly 
225                 230                 235                 240 


Tyr Thr Lys Tyr Asn Ser Val Arg Gly Ala Ser Tyr Asp Asp Phe Lys 
                245                 250                 255     


Lys Glu Val Glu Lys Ile Phe Lys Gly Thr Val Ala Glu Glu Asp Ile 
            260                 265                 270         


Arg Tyr Val Asp Met Met Gln Arg Phe Ala Glu Gly Thr Phe Leu Arg 
        275                 280                 285             


Arg Leu Lys Thr Ser Asp Asn Gly Ser Ile Pro Tyr Gln Leu His Leu 
    290                 295                 300                 


Glu Glu Met Asp Ala Ile Val Glu Asn Gln Lys Gln Phe Tyr Pro Phe 
305                 310                 315                 320 


Leu Glu Ser Glu Lys Glu Lys Leu Glu Ser Leu Val Thr Phe Arg Ile 
                325                 330                 335     


Ser Tyr Tyr Val Gly Pro Leu Thr Gln Lys Asn Ala Arg Leu Asp Asn 
            340                 345                 350         


His Asn Asn Ala Arg Phe Ala Trp Ser Gln Arg Lys Glu Gly Lys Glu 
        355                 360                 365             


His Ala Arg Val Tyr Pro Trp Asn Trp Glu Glu Val Ile Asp Arg Lys 
    370                 375                 380                 


Gln Ser Ala Ala Lys Phe Met Glu Arg Leu Thr Gly Thr Cys Thr Tyr 
385                 390                 395                 400 


Ile Leu Asp Glu Pro Val Leu Pro Arg Cys Ser Leu Leu Tyr Glu Glu 
                405                 410                 415     


Tyr Cys Leu Leu Asn Glu Leu Asn Gly Ser Lys Trp Arg Asp Met Gly 
            420                 425                 430         


Gly Asp Trp His Arg Phe Asp Tyr Glu Thr Arg Met Glu Ile Ile Glu 
        435                 440                 445             


Asn Leu Phe Lys Lys Lys Lys Gly Ser Val Lys Tyr Ala Ala Val Glu 
    450                 455                 460                 


Asp Trp Leu Lys Cys Glu Lys His Lys Ser Cys Ala Glu Val Lys Gly 
465                 470                 475                 480 


Gly Gln Gly Glu Thr Gly Phe Glu Ser Lys Leu Ser Ser Tyr Val Phe 
                485                 490                 495     


Phe Lys Glu Val Leu His Val Asp Glu Ile Asp Glu Ala Asp Ile Pro 
            500                 505                 510         


Met Ile Glu Glu Leu Ile Trp Trp Asn Thr Leu Phe Glu Asp Arg Ser 
        515                 520                 525             


Ile Leu Lys Glu Ser Ile Glu Asp Lys Tyr Gly Asp Arg Leu Thr Asp 
    530                 535                 540                 


Asp Gln Ile Lys Ala Ile Cys Lys Lys Arg Phe Thr Gly Trp Gly Arg 
545                 550                 555                 560 


Leu Ser Lys Glu Leu Leu Cys Gly Ile Lys Thr His Thr Asp Asn Gly 
                565                 570                 575     


Pro Tyr Ser Ile Met Asp Val Leu Arg Glu Gly Asn Pro Asn Asn Asn 
            580                 585                 590         


Ala Arg Ser Glu Thr Met Val Phe Met Glu Ile Leu Arg Asp Asp Lys 
        595                 600                 605             


Phe Gly Phe Asp Lys Leu Ile Glu Glu Glu Asn Met Arg Arg Val Ser 
    610                 615                 620                 


Glu Val Asp Asn Phe Asp Val Asn Asp Leu Pro Gly Ser Pro Ala Val 
625                 630                 635                 640 


Arg Arg Thr Val Asn Gln Ala Lys Arg Ile Val Asp Glu Ile Val Gly 
                645                 650                 655     


Ile Ala Gly Cys Glu Pro Gln Asn Ile Phe Ile Glu Val Thr Arg Asp 
            660                 665                 670         


Glu Asp Gly Arg Asn Lys Gly Arg Arg Thr Lys Arg Arg Tyr Asp Asn 
        675                 680                 685             


Ile Lys Asn Ala Ile Ala Ala Leu Lys Lys Glu Gln Lys Glu Tyr Phe 
    690                 695                 700                 


Asn Lys Asp Val Ser Asp Ser Leu Asn Lys Thr Asn Pro Ser Asp Leu 
705                 710                 715                 720 


Asp Asn Glu Arg Leu Ala Leu Tyr Phe Met Gln Asn Gly Lys Cys Met 
                725                 730                 735     


Tyr Cys Gly Lys Asp Leu Asp Ile Lys Gln Leu Asp Lys Tyr Gln Val 
            740                 745                 750         


Asp His Ile Leu Pro Gln Ser Tyr Val Lys Asp Asp Ser Phe Glu Asn 
        755                 760                 765             


Lys Ala Leu Val His Met Glu Asp Asn Gln Arg Lys Ser Ala Ser Met 
    770                 775                 780                 


Leu Leu Asp Asp Ser Ile Ile Arg Lys Met Lys Pro Tyr Trp Glu Ala 
785                 790                 795                 800 


Leu Arg Ala Ala Gly Leu Ile Gly Glu Lys Lys Phe Lys Asn Leu Thr 
                805                 810                 815     


Arg Val Arg Val Ser Asp Lys Glu Leu Gly Gly Phe Ile Ala Arg Gln 
            820                 825                 830         


Met Val Glu Thr Ser Gln Ser Met Lys Leu Thr Ser Leu Val Leu Glu 
        835                 840                 845             


Lys Thr Tyr Pro Asn Thr Asn Val Arg Pro Val Lys Ala Gly Phe Ser 
    850                 855                 860                 


Ser Asp Leu Arg Lys Arg Leu Asp Leu Ile Lys Cys Arg Glu Ile Asn 
865                 870                 875                 880 


Asp Tyr His His Ala His Asp Ala Tyr Leu Ala Cys Glu Ile Gly Gln 
                885                 890                 895     


Phe Ile Thr Met Arg His Asn Lys Leu Tyr Glu Asn Pro Ile Ser Tyr 
            900                 905                 910         


Ser His Ala Val Lys Asp Phe Ile Ala Lys Arg Ala Lys Tyr Ala Asn 
        915                 920                 925             


Asp His Gly Thr Pro Met Ser Thr Tyr Gly Met Pro Gly Ser Ser Ser 
    930                 935                 940                 


Phe Val Ile Ser Ser Phe Leu Thr Ser Gly Phe Asp Glu Glu Thr Gly 
945                 950                 955                 960 


Glu Ile Phe Lys Asp Ala Trp Ser Ala Glu Val Ala Ala Val Arg Ile 
                965                 970                 975     


Arg Glu Ser Leu Asn Arg Lys Asp Cys Tyr Ile Ser Arg Met Pro Glu 
            980                 985                 990         


Glu Thr Ser Gly Ala Phe Trp Lys  Glu Thr Leu Ile Ser  Pro Arg Asp 
        995                 1000                 1005             


Pro Ser  Leu Gly Lys Asn Leu  Ser Leu Pro Ile Lys  Lys Gly Leu 
    1010                 1015                 1020             


Asp Pro  Arg Lys Tyr Gly Gly  Tyr Asn Glu Gln Lys  Phe Ala Tyr 
    1025                 1030                 1035             


Phe Phe  Val Tyr Glu Ala Val  Lys Ile Lys Lys Lys  Gly Glu Val 
    1040                 1045                 1050             


Lys Glu  Tyr Val Phe Ala Ala  Val Pro Val Gln Val  Ala Ala Ile 
    1055                 1060                 1065             


Val Ala  Arg Asp Pro Asn Ala  Leu Thr Gly Phe Ala  Lys Lys Asp 
    1070                 1075                 1080             


Ala Glu  Ala Arg Lys Ile Lys  Phe Leu Arg Ile Ile  Arg Glu Lys 
    1085                 1090                 1095             


Val Tyr  Lys Tyr Gln Leu Ile  Glu Val Asn Gly Glu  Arg Leu Tyr 
    1100                 1105                 1110             


Ile Thr  Gly Leu Lys Ser Val  Arg Asn Ala Arg Gln  Ile Ala Leu 
    1115                 1120                 1125             


Ser Gln  Asp Met Thr Thr Val  Phe Leu Lys Ala Ile  Asn Asp Lys 
    1130                 1135                 1140             


Asp Val  Ala Glu Asp Glu Leu  Lys Ser Leu Phe Glu  Glu Ile Val 
    1145                 1150                 1155             


Gln Arg  Tyr Arg Leu Cys Ala  Asn Arg Leu Gly Asn  Gln Leu Lys 
    1160                 1165                 1170             


Ile Phe  Ser Leu Ser Asp Ser  Phe Asn Ser Ala Asn  Ser Glu Ser 
    1175                 1180                 1185             


Lys Lys  Arg Ala Leu Val Ser  Leu Val Ala Ile Ile  Asp Ala Lys 
    1190                 1195                 1200             


Thr Asn  Lys Ile Asp Leu Ser  Gly Ile Gly Ser Gly  Lys Ala Val 
    1205                 1210                 1215             


Gly Asn  Met Arg Leu Ser Phe  Ser Lys Glu Met Ser  Asn Ser Lys 
    1220                 1225                 1230             


Asn His  Phe Ala Phe Ile Asp  Gln Ser Val Thr Gly  Met Phe Glu 
    1235                 1240                 1245             


Arg Lys  Thr Tyr Leu 
    1250             


<210>  794
<211>  1258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  794

Met Thr Glu Glu Asn Pro Asp Ile Arg Lys Val Phe Leu Ala Ile His 
1               5                   10                  15      


His Ile Leu Lys Asn Arg Gly His Phe Leu Leu Gln Gly Gln Ser Phe 
            20                  25                  30          


Lys Glu Gly Asn Leu Asn Asn Leu Ile Lys Glu Leu Leu Asn Leu Asp 
        35                  40                  45              


Ile Leu His Ile Ser Ile Glu Ile Thr Glu Glu Asn Ile Asn Lys Ile 
    50                  55                  60                  


Thr Glu Ile Ala Leu Glu Lys Lys Thr Leu Thr Asp Lys Val Asn Asp 
65                  70                  75                  80  


Leu Lys Lys Ile Tyr Pro Lys Glu Lys Gln Leu Leu Glu Ala Phe Arg 
                85                  90                  95      


Leu Ile Phe Gly Gly Lys Thr Ser Leu Asp Lys Leu Phe Ala Ile Asp 
            100                 105                 110         


Glu Tyr Lys Glu Leu Asp Ala Ser Val Lys Ser Val Ser Phe Lys Glu 
        115                 120                 125             


Lys Ile Tyr Glu Glu Val Arg His Asp Tyr Glu Gln Val Leu Ser Asn 
    130                 135                 140                 


Tyr Ile Glu Leu Leu Asp Leu Ala Lys Leu Val Tyr Asp Ser Ile Ile 
145                 150                 155                 160 


Leu Ser Asp Ile Lys Lys Glu Gly Lys Thr Leu Ser Glu Ser Lys Val 
                165                 170                 175     


Glu Leu Tyr His Lys His Arg Glu Asp Leu Thr Lys Leu Lys Thr Leu 
            180                 185                 190         


Val Lys Asn Asp Ser Lys Leu Ser Glu Asp Lys Lys Val Glu Leu Tyr 
        195                 200                 205             


Ser Ala Ile Phe Lys Glu Asp Lys Asp Lys Gly Thr Asn Tyr Val Asn 
    210                 215                 220                 


Tyr Ile Arg Lys Ser Glu Glu Gly Lys Gly Cys Asn Tyr Glu Asp Phe 
225                 230                 235                 240 


Lys Lys Phe Leu Val Lys Glu Leu Ala Lys Leu Glu Glu Ser Ser Ala 
                245                 250                 255     


Lys Glu Glu Ile Leu Arg Asp Leu Glu Leu Glu Leu Phe Leu Pro Leu 
            260                 265                 270         


Gln Arg Thr Lys Asp Asn Ser Val Val Pro Tyr Gln Ile His Lys Glu 
        275                 280                 285             


Glu Leu Val Lys Ile Leu Asp Asn Ala Ala Lys Tyr His Lys Phe Leu 
    290                 295                 300                 


Asn Glu Lys Asp Asp Ser Gly Tyr Ser Ile Ile Glu Lys Val Ile Gln 
305                 310                 315                 320 


Leu Leu Glu Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Ser Ser 
                325                 330                 335     


Lys Lys Ala Lys Val Asp Gly Phe Ala Trp Ser Val Arg Asn Lys Gly 
            340                 345                 350         


Tyr Glu Asn Thr Pro Val Thr Pro Trp Asn Tyr Glu Lys Val Ile Asp 
        355                 360                 365             


Glu Ser Ala Ser Ala Glu Lys Phe Ile Thr Asn Leu Thr Asn Lys Cys 
    370                 375                 380                 


Thr Tyr Leu Lys Gly Glu Asp Val Leu Pro Lys Ser Ser Leu Leu Tyr 
385                 390                 395                 400 


Ser Glu Phe Ala Leu Leu Asn Glu Leu Asn Ala Leu Lys Tyr Asp Gly 
                405                 410                 415     


Asn Arg Ile Ser Leu Glu Ala Arg Asn Val Ile Ile Glu Lys Leu Phe 
            420                 425                 430         


Lys Glu Gln Gly Lys Lys Val Thr Lys Thr Ser Ile Lys Asn Leu Leu 
        435                 440                 445             


Lys Ala Glu Gly Tyr Ile Asp Gly Lys Gly Glu Ile Thr Gly Ile Asp 
    450                 455                 460                 


Ile Thr Val Lys Asn Asp Leu Lys Ser Tyr Arg Asp Phe Lys Lys Ile 
465                 470                 475                 480 


Leu Gly Asp Lys Phe Asn Thr Glu His Val Glu Asn Ile Ile Leu Trp 
                485                 490                 495     


Ile Thr Leu Tyr Gly Glu Ser Arg Lys Leu Ile Lys Ala Lys Ile Glu 
            500                 505                 510         


Ala Val Tyr Gly Asp Ile Tyr Ser Lys Glu Glu Ile Ala Lys Met Ser 
        515                 520                 525             


Arg Leu Val Tyr Lys Asp Trp Gly Arg Phe Ser Arg Lys Leu Leu Thr 
    530                 535                 540                 


Glu Leu Val Ser Lys Lys Leu Tyr Asn Glu Glu Thr Gly Glu Cys Leu 
545                 550                 555                 560 


Asn Ile Ile Gly Ala Met Arg Gln Asn Asn Ile Leu Phe Met Glu Leu 
                565                 570                 575     


Leu Ala Asp Arg Phe Asp Tyr Ser Gln Gln Ile Ile Asp Tyr Asn Lys 
            580                 585                 590         


Glu Leu Gln Lys Glu Val Thr Glu Ile Thr Pro Glu Ile Leu Asp Asp 
        595                 600                 605             


Leu Tyr Val Ser Pro Ser Val Lys Arg Ser Ile Trp Gln Thr Val Arg 
    610                 615                 620                 


Ile Val Glu Glu Leu Lys Lys Ile Ile Gly Cys Ala Pro Ala Lys Ile 
625                 630                 635                 640 


Phe Val Glu Thr Thr Arg Ser Asn Gln Asp Lys Lys Lys Pro Thr Asp 
                645                 650                 655     


Ser Arg Lys Lys Gln Leu Glu Leu Phe Tyr Lys Ala Val Lys Lys Asp 
            660                 665                 670         


Val Lys Asp Leu Glu Lys Glu Ile Gly Ser Leu Asn Phe Asp Lys Leu 
        675                 680                 685             


Asn Glu Arg Leu Ser Ser Val Glu Pro Ser Lys Leu Lys Ala Lys Lys 
    690                 695                 700                 


Leu Tyr Leu Tyr Tyr Thr Gln Leu Gly Arg Cys Met Tyr Ser Gly Glu 
705                 710                 715                 720 


Thr Ile Asp Ile Thr Gln Leu Asn Thr Thr Ala Tyr Asp Ile Asp His 
                725                 730                 735     


Ile Tyr Pro Gln Ser Lys Val Lys Asp Asp Ser Phe Thr Asn Thr Val 
            740                 745                 750         


Leu Val Lys Arg Glu Tyr Asn Ala Ala Lys Thr Asp Arg Tyr Pro Leu 
        755                 760                 765             


Gly Ser Asp Ile Gln Thr Pro Ser Asn Arg Arg Phe Trp Lys Phe Leu 
    770                 775                 780                 


Lys Glu Lys Gly Leu Ile Thr Asp Glu Lys Tyr Asn Arg Leu Val Arg 
785                 790                 795                 800 


Thr Glu Glu Phe Ser Asp Glu Glu Leu Thr Gly Phe Ile Ala Arg Gln 
                805                 810                 815     


Leu Val Glu Thr Ser Gln Ser Val Lys Ala Val Ala Gly Ile Leu Gly 
            820                 825                 830         


Glu Leu Asn Pro Lys Thr Ser Ile Cys Tyr Ser Lys Ala Glu Asn Val 
        835                 840                 845             


Ser Ser Phe Arg Gln Asn Phe Gly Lys Ile Lys Glu Gly Asn Arg Lys 
    850                 855                 860                 


Ser Asp Asn Thr Glu Gln Leu Ile Lys Val Arg Glu Ile Asn Asp Tyr 
865                 870                 875                 880 


His His Ala Lys Asp Ala Tyr Leu Asn Val Val Val Gly Asn Val Tyr 
                885                 890                 895     


Asp Thr Lys Phe Thr Arg Asn Val Phe Asn Phe Ile Lys Asp Lys Lys 
            900                 905                 910         


Asp Gly Arg Lys Tyr Ser Leu Asn Asn Val Phe Tyr Glu Asn Val Ser 
        915                 920                 925             


Asn Ser Asn Asn Val Ala Trp Glu Leu Asn Lys Thr Val His Gln Val 
    930                 935                 940                 


Glu Lys Val Met Asn Asn Asn Asn Ile Leu Val Thr Arg Arg Thr Ser 
945                 950                 955                 960 


Glu Gln Lys Gly Gly Leu Phe Asp Ala Thr Val Tyr Lys Ala Lys Val 
                965                 970                 975     


Ala Ala Lys Ala Lys Glu Gly Val Tyr Tyr Pro Leu Lys Thr Ser Asp 
            980                 985                 990         


Pro Val Val Asn Asp Val Thr Lys  Tyr Gly Gly Phe Thr  Lys Val Lys 
        995                 1000                 1005             


Ile Ala  Tyr Tyr Ser Ile Phe  Glu Tyr Thr Leu Val  Asn Lys Lys 
    1010                 1015                 1020             


Gly Glu  Glu Lys Ile Thr Arg  Ile Ile Pro Ile Pro  Ile Tyr Val 
    1025                 1030                 1035             


Ser Gln  Asn Ile Lys Asp Asp  Ser Thr Leu Leu Glu  Phe Gly Lys 
    1040                 1045                 1050             


Thr Gln  Ile Asn Ile Lys Ser  Gly Glu Glu Val Lys  Asp Phe Lys 
    1055                 1060                 1065             


Leu Lys  Tyr Arg Lys Leu Cys  Ile Gly Asp Leu Val  Lys Ile Asn 
    1070                 1075                 1080             


Asn Phe  Phe Asn Phe Ile Gly  Gly Lys Thr Asn Asp  Ser Phe Val 
    1085                 1090                 1095             


Tyr Asp  Gly Ala Val Gln Val  Thr Leu Asp Thr Glu  Ser Glu Arg 
    1100                 1105                 1110             


Tyr Ile  Lys Thr Leu Ser Lys  Tyr Gln Asn Trp Lys  Lys Glu Asn 
    1115                 1120                 1125             


Lys Asp  Gly Glu Leu Trp Glu  Ser Ile Thr Lys Glu  Lys Asn Thr 
    1130                 1135                 1140             


Glu Leu  Tyr Asn Leu Leu Val  Glu Lys Met Asn Ser  Gly Ile Phe 
    1145                 1150                 1155             


Ile Lys  Lys Lys Pro Asn Lys  Phe Asn Glu Leu Asn  Ser Glu Glu 
    1160                 1165                 1170             


Ile Arg  Asn Asn Phe Ser Ser  Ile Ser Leu Glu Glu  Gln Ala Lys 
    1175                 1180                 1185             


Val Leu  Leu Glu Val Leu Asn  Leu Leu Thr Asn Lys  Lys Ser Ile 
    1190                 1195                 1200             


Phe Asp  Leu Lys Ser Ile Asn  Ile Thr Ala Ala Arg  Gly Lys Ile 
    1205                 1210                 1215             


Ser Phe  Asn Leu Ser Asn Leu  Ser Gln Phe Ser Ile  Ile Glu Gln 
    1220                 1225                 1230             


Ser Val  Ala Gly Phe Tyr Glu  Lys Glu Ile Thr Ile  Ile Gly Asp 
    1235                 1240                 1245             


Lys Glu  Asn Asp Met Glu Asn  Asn Asn Cys 
    1250                 1255             


<210>  795
<211>  1259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  795

Met Lys Tyr Ile Leu Gly Leu Asp Leu Gly Val Ala Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Thr Glu Ile Asn Glu Lys Gly Glu Pro Val Gly Leu Ile Asp 
            20                  25                  30          


Ala Asn Ser Val Ile Phe Lys Pro Leu Asp Asn Asp Lys Gly Lys Leu 
        35                  40                  45              


Tyr Asn Val Glu Arg Arg Asp Lys Arg Gly Ser Arg Arg Ile Leu Arg 
    50                  55                  60                  


Arg Lys Lys His Arg Val Glu Arg Thr Lys Met Leu Leu Val Asn Ser 
65                  70                  75                  80  


Ser Phe Leu Thr Asn Glu Glu Ile Asp Asn Leu Tyr Val Gly Lys Leu 
                85                  90                  95      


Asp Asn Ile Tyr Glu Val Arg Leu Lys Gly Leu Lys Glu Gln Leu Ser 
            100                 105                 110         


Lys Asn Glu Ile Ala Arg Leu Met Ile Tyr Tyr Cys Lys Asn Arg Gly 
        115                 120                 125             


Phe Lys Ser Asn Arg Lys Thr Glu Asp Leu Glu Ile Leu Lys Ser Leu 
    130                 135                 140                 


Gly Glu Lys Ile Ser Glu Lys Asp Ser Asp Glu Lys Lys Leu Lys Pro 
145                 150                 155                 160 


Ile Ile Ala Lys Asn Thr Glu Leu Ile Glu Ser Lys Gly Leu Thr Pro 
                165                 170                 175     


Ile Glu Val Ile Tyr Met Ile Arg Glu Asn Asp Asn Ser Ile Leu Gly 
            180                 185                 190         


Phe Lys Asn Lys Glu Gly Asn Tyr Lys Phe Gly Phe Lys Arg Asn Gln 
        195                 200                 205             


Val Ile Asp Glu Val Arg Lys Ile Leu Gly Thr Gln Gln Ile Leu Ser 
    210                 215                 220                 


Lys Glu Ile Val Asp Glu Tyr Ile Asp Ile Leu Ser Ser Gln Arg Asp 
225                 230                 235                 240 


Phe Ser Asp Gly Pro Gly Gly Asp Ser Lys Tyr Lys Ile Asp Tyr Thr 
                245                 250                 255     


Lys Leu Ala Gly Lys Cys Lys Tyr Thr Gly Glu Val Arg Ala Val Lys 
            260                 265                 270         


Ser Ala Pro Ser Tyr Glu Ile Phe Thr Met Leu Gln Lys Leu Asn Asp 
        275                 280                 285             


Ile Arg Tyr Val Lys Phe Thr Ser Glu Asp Lys Ile Glu Lys Lys Lys 
    290                 295                 300                 


Leu Ser Lys Glu Val Ile His Lys Leu Tyr Asp Leu Val Val Glu Lys 
305                 310                 315                 320 


Asn Lys Thr Leu Thr Tyr Asp Leu Ile Glu Lys Ser Ile Asp Glu Glu 
                325                 330                 335     


Asn Ile Lys Leu Leu Asn Ile Pro Lys Leu Ser Lys Ser Lys Tyr Val 
            340                 345                 350         


Glu Leu Arg Lys Ser Tyr Phe Lys Glu Arg Asn Asp Asn Glu Asn Leu 
        355                 360                 365             


Thr Glu Glu Glu Ile Asn Ala Phe Asn Glu Lys Leu Asn Asn Glu Arg 
    370                 375                 380                 


Met Lys Gln Glu Leu Ile Arg Asn Asn Leu Lys Ala Tyr Lys Glu Leu 
385                 390                 395                 400 


Lys Ser Lys Phe Lys Lys Asn Asn Ile Asp Glu Asn Arg Ile Lys Glu 
                405                 410                 415     


Leu Gly Gly Ile Tyr Phe Leu Asp Leu Val Ala Glu Leu Leu Thr Tyr 
            420                 425                 430         


Ser Lys Thr Asp Glu Lys Leu Glu Tyr Phe Val Glu Asn Val Glu Arg 
        435                 440                 445             


Tyr Ser Leu Phe Lys Glu Gln Arg Asp Ile Val Glu Ile Ile Lys Thr 
    450                 455                 460                 


Phe Pro Asn Tyr Thr Lys Asn Gly Asn Leu Ser Leu Ser Leu Val Arg 
465                 470                 475                 480 


Glu Leu Asn Lys Leu Leu Met Glu Gly His Asp Tyr Glu Ser Ser Leu 
                485                 490                 495     


Ser Ser Leu Asn Tyr Tyr Ile Asp Thr Asp Val Asp Trp Glu Lys Phe 
            500                 505                 510         


Pro Thr Ile Ser Glu Ile Glu Asp Ser Leu Ser Thr Lys Ile Thr Asn 
        515                 520                 525             


Pro Asn Val Lys His Ile Leu Val Ile Leu Arg Lys Leu Tyr Asn Thr 
    530                 535                 540                 


Leu Leu Phe Lys Tyr Gly Arg Pro Glu Lys Val His Leu Glu Leu Ser 
545                 550                 555                 560 


Arg Asp Phe Ser Asn Asp Phe Ser Thr Arg Asn Lys Ile Gln Lys Glu 
                565                 570                 575     


Gln Leu Glu Asn Lys Val Arg Arg Glu Val Ala Ala Phe Glu Met Tyr 
            580                 585                 590         


Gly Ala Asn Lys Asp Ile Val Ala Gly Lys Asp Arg Leu Ser Asn Asp 
        595                 600                 605             


Asp Phe Val Arg Ile Lys Leu Trp Glu Glu Gln Asn Lys Val Cys Met 
    610                 615                 620                 


Tyr Ser Gly Arg Thr Ile Glu Lys Tyr Gln Leu Thr Ser Ala Glu Val 
625                 630                 635                 640 


Gln Ile Asp His Ile Leu Pro Tyr Ser Lys Ser Phe Asp Asn Ser Tyr 
                645                 650                 655     


Ser Asn Lys Val Leu Val Phe Ser Asn Glu Asn Gln Asp Lys Lys Glu 
            660                 665                 670         


Arg Thr Pro Tyr Gln Trp Leu Lys Gly Thr Glu Lys Trp Asn Glu Phe 
        675                 680                 685             


Lys Gln Arg Val Arg Leu Asn Leu Asn Ile Ser Asn Lys Lys Lys Glu 
    690                 695                 700                 


Asn Leu Leu Phe Glu Asp Glu Val Val Asn Asn Glu Phe Leu Glu Arg 
705                 710                 715                 720 


Glu Leu His Ala Thr Ser Tyr Ser Ser Arg Leu Ala Leu Asn Ile Phe 
                725                 730                 735     


Gln Arg Leu Ile Pro Val Ser Asp Glu Asp Lys Tyr Asp Glu His Gly 
            740                 745                 750         


Asn Glu Lys Val Lys Tyr Leu Tyr Asn Arg Asn Val Ile Ala Phe Gln 
        755                 760                 765             


Gly Lys Met Thr Ser Met Leu Arg Asn Leu Tyr Ser Leu Asn Lys Tyr 
    770                 775                 780                 


Thr His Ser Phe Glu Ser Asp Thr Leu Asp Ile Asp Asn Lys Ala Phe 
785                 790                 795                 800 


Ile Leu Lys Glu Phe Glu Ile Asn Lys Asp Asn Leu Lys Met Thr Ala 
                805                 810                 815     


Tyr Asn Val Asn Thr Gly Leu Glu Ile Thr Ser Glu Thr Lys Val Glu 
            820                 825                 830         


Lys Asn Lys Ser Gly Glu Phe Lys Thr Ile Lys Asp Glu Leu Leu Lys 
        835                 840                 845             


Lys Glu Leu Asp Lys Lys Glu Asn Leu Ile Glu Ile Ser Asp Tyr Phe 
    850                 855                 860                 


Lys Asp Lys Val Leu Phe Asp Leu Lys Ile Val Asp Phe Glu Glu Ile 
865                 870                 875                 880 


Asp Thr Val Glu Pro Glu Ile Ile Ser Ile Leu Tyr Lys Met Ile Thr 
                885                 890                 895     


Ala Leu Lys Glu Glu Val Tyr Ser Lys Asn Arg Glu Asn His Leu His 
            900                 905                 910         


His Ser Leu Asp Ala Phe Leu Leu Thr Ile Met Asn Arg Ser Met Gln 
        915                 920                 925             


Met Lys Leu Ile Lys Tyr Asn Gln Leu Ile Ser Val Leu Lys Asn Lys 
    930                 935                 940                 


Glu Ile Glu Ile Phe Asn Glu Glu Lys Gly Glu Tyr Ile Asp Ser Lys 
945                 950                 955                 960 


Glu Phe Ala Glu Glu Leu Gln Lys Gln Lys Ile Ile Asp Ile Asp Gly 
                965                 970                 975     


Asn Glu Arg Gly Ile Lys Phe Asn Val Asn Gly Lys Thr His Arg Leu 
            980                 985                 990         


Asn Leu Val Lys Pro Tyr Glu Asn  Phe Leu Asp Asp Ile  Lys Asn Lys 
        995                 1000                 1005             


Ile Phe  Asp Lys Arg Glu Asn  Asn Lys Leu Pro Tyr  His Val Val 
    1010                 1015                 1020             


Lys Ser  Lys Val Ser Gly Ala  Leu His Ala Glu Thr  Ile Leu Gly 
    1025                 1030                 1035             


Glu Ser  Lys Gly Glu Ile Thr  Lys Arg Ile Ser Val  Phe Asp Ile 
    1040                 1045                 1050             


Asn Tyr  Lys Asn Leu Gly Lys  Ile Phe Asp Lys Asp  Gly Ser Gln 
    1055                 1060                 1065             


Lys Glu  Ile Tyr Glu Thr Leu  Val Lys Trp Leu Glu  Ala Lys Ser 
    1070                 1075                 1080             


Lys Asp  Tyr Pro Lys Leu Lys  Asn Gly Asn Ile Ile  Lys Lys Val 
    1085                 1090                 1095             


Lys Ile  Val Asp Gly Asn Lys  Asp Lys Leu Ile Lys  Leu Gly Asn 
    1100                 1105                 1110             


Lys Arg  Tyr Val Glu Met Gly  Arg Thr Thr Val Lys  Ile Leu Val 
    1115                 1120                 1125             


Leu Lys  Lys Glu Ala Glu Glu  Gly Leu Lys Phe Ala  Ser Ile Gly 
    1130                 1135                 1140             


Arg Tyr  Lys Tyr Asn Leu Leu  Lys Ser Glu Lys Asp  Val Asn Ile 
    1145                 1150                 1155             


Ser Ile  Trp Lys Asp Ala Ile  His Phe Cys Lys Val  Arg Tyr Asn 
    1160                 1165                 1170             


Lys Leu  Lys Glu Asn Gly Tyr  Arg Leu Ile His Glu  Leu Ile Pro 
    1175                 1180                 1185             


Gly Glu  Thr Ile Glu Leu Glu  Leu Asn Lys Gly Asn  Ile Ser Lys 
    1190                 1195                 1200             


Cys Leu  Val Val Gly Phe Ser  Gly Gly Lys Ile Glu  Ile Ser Ser 
    1205                 1210                 1215             


Val Ile  Gly Asp Ala Leu Asp  Leu Ile Asn Asp Lys  Ile Ser Thr 
    1220                 1225                 1230             


Arg Ile  Lys Glu Arg Tyr Tyr  Ile Thr Val Ser Thr  Ile Lys Ser 
    1235                 1240                 1245             


Ile Lys  Lys Ile Asn Lys Asn  Ile Leu Gly Asp 
    1250                 1255                 


<210>  796
<211>  1263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  796

Met His Lys Asn Asn Pro Asp Ile Arg Glu Leu Phe Leu Ala Ile His 
1               5                   10                  15      


His Ile Leu Lys Tyr Arg Gly His Phe Leu Phe Ala Gly Gln Gln Phe 
            20                  25                  30          


Lys Gly Ser Glu Ile Lys Asp Asn Ile Arg Glu Leu Leu Gln Tyr Asn 
        35                  40                  45              


Ser Leu Asn Ile Lys Ile Asp Ile Thr Asp Glu Leu Ile Asn Asn Ile 
    50                  55                  60                  


Ala Glu Ile Leu Leu Lys Lys Lys Asn Ser Thr Asp Lys Ile Ile Glu 
65                  70                  75                  80  


Phe Lys Lys Leu Phe Ala Asp Glu Lys Gln Leu Ile Glu Ala Leu Arg 
                85                  90                  95      


Leu Ala Leu Gly Asn Lys Thr Gln Leu Ala Lys Leu Phe Asp Ile Glu 
            100                 105                 110         


Ala Tyr Lys Glu Leu Asp Ile Ser Ile Lys Thr Ile Ser Phe Lys Glu 
        115                 120                 125             


Lys Ile Tyr Glu Asp Val Arg Ser Asn Tyr Glu Glu Ile Leu Gly Glu 
    130                 135                 140                 


Tyr Ile Ile Val Leu Asp Thr Ala Lys Leu Ile Tyr Asp Ala Ile Ile 
145                 150                 155                 160 


Leu Ser Glu Ile Lys Ile Lys Asn Met Thr Ile Ser Glu Ser Lys Val 
                165                 170                 175     


Ile Ser Tyr Glu Lys His Gly Lys Asp Leu Lys Gln Leu Lys Gln Leu 
            180                 185                 190         


Ile Lys Glu Asp Ser Lys Leu Ser Lys Lys Glu Lys Arg Gln Leu Phe 
        195                 200                 205             


Glu Asn Ile Phe Val Lys Glu Asp Glu Lys Glu Ala Asn Tyr Val Asn 
    210                 215                 220                 


Tyr Ile Gly Lys Asn Asn Val Gly Ser Ser Lys Ser Ser Cys Ser Tyr 
225                 230                 235                 240 


Glu Asp Phe Lys Lys Ser Leu Ile Lys Glu Leu Lys Tyr Cys Glu Glu 
                245                 250                 255     


Ser Lys Leu Lys Gln Asp Ile Leu Ala Glu Leu Glu Leu Glu Thr Phe 
            260                 265                 270         


Leu Pro Arg Gln Arg Thr Lys Asp Asn Ser Val Ile Pro Tyr Gln Ile 
        275                 280                 285             


His Lys Glu Glu Leu Glu Lys Ile Leu Asn Asn Ala Val Gln Tyr His 
    290                 295                 300                 


Pro Phe Leu Asn Asn Thr Asp Glu Tyr Gly Ile Ser Ile Lys Glu Lys 
305                 310                 315                 320 


Ile Ile Gln Leu Leu Glu Phe Arg Ile Pro Tyr Tyr Val Gly Ser Leu 
                325                 330                 335     


Asn Thr Ser His Lys Val Gly Asn Gly Gly Phe Ser Trp Ser Val Arg 
            340                 345                 350         


Asn Lys Gly Tyr Glu Arg Thr Ala Val Thr Pro Trp Asn Phe Asn Lys 
        355                 360                 365             


Val Ile Asn Lys Glu Glu Ser Ala Lys Asn Phe Ile Glu Asn Leu Thr 
    370                 375                 380                 


Asn Gln Cys Thr Tyr Leu Leu Tyr Glu Asp Val Leu Pro Lys His Ser 
385                 390                 395                 400 


Leu Leu Tyr Ser Glu Phe Thr Leu Leu Asn Glu Leu Asn Ser Ile Asn 
                405                 410                 415     


Tyr Gly Gly Lys Arg Leu Thr Val Glu His Arg Asn Leu Ile Ile Glu 
            420                 425                 430         


Arg Phe Phe Lys Gln Ser Ala Pro Lys Ala Thr Gly Lys Glu Leu Lys 
        435                 440                 445             


Lys Phe Leu Met Ser Glu Gly Val Ile Asp Gly Lys Asp Glu Leu Ser 
    450                 455                 460                 


Gly Phe Asp Ile Ser Ile Lys Asn Asp Leu Lys Ser Tyr Arg Asp Phe 
465                 470                 475                 480 


Lys Lys Ile Leu Gly Glu Lys Phe Asp Ser Glu Lys Val Glu Lys Met 
                485                 490                 495     


Ile Arg Trp Ile Thr Leu Phe Gly Glu Ser Lys Gln Leu Leu Val Asp 
            500                 505                 510         


Lys Ile Thr Lys Glu Tyr Gly Asp Ile Tyr Ser Ser Glu Glu Ile Lys 
        515                 520                 525             


Lys Ile Ser Gly Leu Thr Tyr Lys Asp Trp Gly Arg Leu Ser Glu Lys 
    530                 535                 540                 


Leu Leu Thr Glu Ile Val Ser Ser Gln Leu Tyr Asp Glu Ser Thr Gly 
545                 550                 555                 560 


Glu Cys Phe Asn Ile Ile Asn Ala Met Arg Asn Asn Thr Leu Leu Phe 
                565                 570                 575     


Ile Glu Leu Leu Gly Thr Asn Tyr Asp Tyr Phe Glu Lys Ile Asn Thr 
            580                 585                 590         


Tyr Asn Gly Lys Leu Arg Lys Glu Ile Thr Glu Ile Thr Pro His Ile 
        595                 600                 605             


Leu Asp Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ser Ile Trp Gln 
    610                 615                 620                 


Val Val Arg Ile Val Glu Glu Leu Lys Lys Val Ile Gly Ser Glu Pro 
625                 630                 635                 640 


Lys Arg Ile Phe Val Glu Thr Thr Arg Ser Asn Lys Glu Lys Lys Gly 
                645                 650                 655     


Asp Asp Gly Arg Lys Asp Ser Arg Lys Lys Ala Leu Leu Glu Leu Tyr 
            660                 665                 670         


Lys Lys Ile Lys Thr Ser Asp Ile Ala Glu Leu Glu Lys Ala Leu Gly 
        675                 680                 685             


His Thr Val Asp Phe Asp Lys Lys Lys Gln Asp Leu Thr Asp Ile Asp 
    690                 695                 700                 


Asn Asn Lys Leu Lys Ala Arg Lys Leu Tyr Leu Tyr Tyr Thr Gln Leu 
705                 710                 715                 720 


Gly Arg Cys Leu Tyr Thr Gly Lys Ala Ile Asp Leu Asn Asn Leu Phe 
                725                 730                 735     


Gly Ser Asn Ser Glu Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys 
            740                 745                 750         


Thr Lys Asp Gly Ser Trp Asn Asn Leu Val Leu Val Asn Lys Thr Glu 
        755                 760                 765             


Asn Ser Lys Lys Ser Asn Gly Leu Ile Ser Glu Ser Ile Gln Arg Lys 
    770                 775                 780                 


Asn Lys His Phe Trp Arg Phe Leu Lys Ala Lys Asn Leu Ile Ser Glu 
785                 790                 795                 800 


Glu Lys Tyr Lys Arg Leu Thr Arg Thr Glu Asp Phe Thr Asp Gly Glu 
                805                 810                 815     


Leu Ser Gly Phe Ile Ala Arg Gln Leu Ile Glu Thr Ser Gln Ser Val 
            820                 825                 830         


Lys Val Val Ser Lys Ile Leu Gln Asn Leu Asn Pro Thr Thr Glu Leu 
        835                 840                 845             


Cys Phe Ser Lys Ala Glu Asn Val Ser Asp Phe Arg Gln Glu Phe Gly 
    850                 855                 860                 


Lys Ile Lys Val Gly Lys Arg Lys Asn Leu Thr Glu Glu Glu Gln Lys 
865                 870                 875                 880 


Lys Ala Lys Glu Gly Gln Leu Ile Lys Val Arg Asp Leu Asn Asp Leu 
                885                 890                 895     


His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly Asn Val Tyr 
            900                 905                 910         


His Ile Lys Phe Thr Arg Asn Pro Lys Asn Phe Ile Arg Lys Asn Phe 
        915                 920                 925             


Lys Asn Arg Glu Tyr Thr Leu Asn Thr Leu Phe Tyr Ser Asp Lys Val 
    930                 935                 940                 


Glu Arg Asn Gly Glu Val Ala Trp Glu Lys Gly Lys Thr Glu Glu Ile 
945                 950                 955                 960 


Val Ser Ser Val Met Cys Asn Asn Leu Ile Asn Val Thr Arg Arg Thr 
                965                 970                 975     


Ser Glu Gln Lys Gly Ala Leu Tyr Glu Ala Thr Ile Tyr Lys Ala Asn 
            980                 985                 990         


Ala Ala Lys Glu Gly Ser Tyr Tyr  Pro Val Lys Thr Asn  Asn Lys Asn 
        995                 1000                 1005             


Leu Lys  Asn Val Thr Arg Tyr  Gly Gly Phe Thr Ser  Ile Lys Ile 
    1010                 1015                 1020             


Ala Tyr  Tyr Ser Ile Phe Lys  Tyr Asp Ile Arg Asn  Lys Asp Lys 
    1025                 1030                 1035             


Val Lys  Glu Asn Ile Lys Ile  Ile Pro Ile Pro Ile  Tyr Ile Ser 
    1040                 1045                 1050             


Gln Asn  Ile Lys Thr Asp Leu  Asp Leu Ile Asn Phe  Ala Glu Lys 
    1055                 1060                 1065             


Gln Ile  Lys Leu Lys Lys Gly  Glu Asn Ile Glu Asn  Leu Gln Leu 
    1070                 1075                 1080             


Ile Tyr  Lys Lys Leu Asn Ile  Gly Ala Leu Val Lys  Ile Asp His 
    1085                 1090                 1095             


Tyr Pro  Tyr Tyr Ile Gly Gly  Lys Thr Asp Asp Asn  Phe Tyr Val 
    1100                 1105                 1110             


Asp Gly  Ala Val Gln Val Leu  Leu Ser Ser Asp Ile  Glu Arg Tyr 
    1115                 1120                 1125             


Leu Lys  Glu Val Val Lys Phe  Val Asn Phe Gln Arg  Glu Asn Lys 
    1130                 1135                 1140             


Glu Ala  Lys Val Ser Lys Glu  His Ile Ser Leu Glu  Glu Asn Ile 
    1145                 1150                 1155             


Lys Leu  Tyr Asn Thr Leu Val  Cys Lys Met Ala Thr  Lys Ile Phe 
    1160                 1165                 1170             


Thr His  Lys Lys Asn Asn Lys  Tyr Asn Asp Leu Thr  Gln Asp Lys 
    1175                 1180                 1185             


Ala Lys  Asn Thr Phe Asn Lys  Leu Ser Leu Glu Glu  Gln Cys Lys 
    1190                 1195                 1200             


Val Leu  Leu Glu Ile Leu Asn  Leu Leu Thr Asn Lys  Lys Thr Thr 
    1205                 1210                 1215             


Phe Asp  Leu Ser Leu Ile Asn  Glu Lys Thr Ser Arg  Val Lys Cys 
    1220                 1225                 1230             


Gly Phe  Asn Leu Leu Asn Gln  Lys Gln Phe Ser Ile  Ile Glu Gln 
    1235                 1240                 1245             


Ser Val  Thr Gly Leu Tyr Glu  Lys Glu Ile Lys Ile  Ile Glu Asp 
    1250                 1255                 1260             


<210>  797
<211>  1264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  797

Met Lys Asn Val Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Val Asp Glu Asn Asn Leu Pro Ile Arg Ile Ile Ala 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Leu Ser Thr Ser Asp Lys Glu Glu Phe 
        35                  40                  45              


Gln Arg Gly Leu Ser Ile Thr Lys Asn Gln Ser Arg Thr Val Ser Arg 
    50                  55                  60                  


Thr Gln Arg Lys Gly Tyr Asp Arg Lys Gln Leu Lys Lys Ser Asp Leu 
65                  70                  75                  80  


Lys Lys Ile Leu Asn Lys Tyr Asn Ile Phe Pro Ser Glu Glu Leu Leu 
                85                  90                  95      


Arg Leu Pro Met Leu Asp Leu Trp Lys Leu Arg Ser Asp Ser Ala Asn 
            100                 105                 110         


Pro Asp Glu Asn Ile Ser Ala Glu Gln Leu Gly Arg Ile Leu Tyr Met 
        115                 120                 125             


Leu Asn Gln Lys Arg Gly Tyr Lys Ser Ala Arg Ser Glu Ala Asn Ala 
    130                 135                 140                 


Asp Lys Lys Asp Ser Asp Tyr Val Gln Ala Val Lys Gly Arg Tyr Asp 
145                 150                 155                 160 


Gln Leu Lys Asp Arg Lys Gln Thr Ile Gly Gln Tyr Phe Tyr Asp Glu 
                165                 170                 175     


Leu Tyr Asn Ala Asn Ala Asn Asn Ser Tyr Phe Arg Ile Lys Glu Glu 
            180                 185                 190         


Val Tyr Pro Arg Glu Ala Tyr Ile Glu Glu Phe Asp Thr Ile Ile Ser 
        195                 200                 205             


Ala Gln Arg Glu Lys His Asp Phe Leu Thr Asp Glu Val Val His Gln 
    210                 215                 220                 


Leu Arg Asn Glu Ile Ile Tyr Phe Gln Arg Lys Leu Lys Ser Gln Lys 
225                 230                 235                 240 


Gly Leu Val Ser Ile Cys Glu Phe Glu Gly Phe Glu Lys Thr Val Phe 
                245                 250                 255     


Asp Lys Glu Lys Asn Lys Asn Lys Val Val Phe Val Gly Pro Lys Val 
            260                 265                 270         


Ala Pro Lys Thr Ser Pro Leu Phe Gln Leu Cys Arg Ile Trp Glu Val 
        275                 280                 285             


Val Asn Thr Ile Thr Leu Lys Ile Lys Asn Pro Glu Gly Ser Lys Tyr 
    290                 295                 300                 


Lys Trp Gly Glu Lys Ile Pro Asp Ile Lys Glu Lys Glu Ile Leu Ala 
305                 310                 315                 320 


Asp Tyr Leu Phe Lys Asn Glu Ser Leu Ser Phe Ser Lys Leu Leu Glu 
                325                 330                 335     


Ile Leu Glu Leu Lys Lys Glu Asn Val Tyr Val Asn Lys Gln Ile Leu 
            340                 345                 350         


Lys Gly Ile Lys Gly Asn Glu Thr Tyr Ser Ala Leu His Ser Ile Leu 
        355                 360                 365             


Gly Asp Asn Glu Leu Leu Asn Phe Asp Val Ser Ile Met Thr Ser Asn 
    370                 375                 380                 


Gln Thr Ala Leu Leu Val Asp Arg Lys Thr Gly Glu Ile Leu Glu Glu 
385                 390                 395                 400 


Arg Ala Gly Leu Glu Leu Asp Ala Ser Leu Glu Lys Glu Pro Leu Tyr 
                405                 410                 415     


Gln Leu Trp His Thr Ile Tyr Ser Leu Lys Asp Leu Asp Glu Cys Lys 
            420                 425                 430         


Asn Ala Leu Ile Lys Arg Phe Asp Phe Asp Asp Glu Thr Ala Glu Lys 
        435                 440                 445             


Leu Ser Arg Ile Asp Phe Asn Lys Gln Ala Phe Gly Asn Lys Ser Asn 
    450                 455                 460                 


Lys Ser Met Arg Lys Ile Leu Pro Phe Leu Met Glu Gly Tyr Asp Tyr 
465                 470                 475                 480 


Ser Gln Ser Ser Ser Leu Ala Gly Tyr Asn His Ser Asn Ser Leu Thr 
                485                 490                 495     


Lys Asn Glu Arg Glu Gln Gln Ile Thr Leu Asp Arg Leu Glu Leu Leu 
            500                 505                 510         


Pro Lys Asn Ser Leu Arg Gln Pro Ile Val Glu Lys Ile Leu Asn Gln 
        515                 520                 525             


Met Ile Asn Val Val Asn Ala Ile Leu Asp Glu Tyr Gly Lys Pro Ser 
    530                 535                 540                 


Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Gln Ser Lys Asp Glu 
545                 550                 555                 560 


Arg Asn Glu Thr Asp Leu Gln Asn Ser Lys Asn Lys Lys Leu Asn Asp 
                565                 570                 575     


Glu Ile Gly Lys Arg Leu Glu Glu Leu Gly Leu Pro Val Thr Lys Arg 
            580                 585                 590         


Tyr Ile Gln Lys Tyr Lys Phe Ile Phe Pro Ser Leu Ser Lys Asn Leu 
        595                 600                 605             


Lys Glu Ala Lys Val Asn Asn Gln Cys Ile Tyr Cys Gly Glu Thr Phe 
    610                 615                 620                 


Asn Leu Thr Glu Ala Leu Ser Gly Asp Ala Tyr Asp Val Asp His Ile 
625                 630                 635                 640 


Val Pro Lys Ala Leu Leu Phe Asp Asp Ser Gln Thr Asn Lys Val Leu 
                645                 650                 655     


Val His Arg Lys Cys Asn Ser Thr Lys Thr Asn Gln Thr Ala Tyr Asp 
            660                 665                 670         


Tyr Ile Ala Thr Lys Gly Asp Glu His Leu Lys Leu Tyr Ser Glu Arg 
        675                 680                 685             


Leu Asp Asp Trp Phe Glu Arg Gly Ile Ile Ser Tyr Ser Lys Met Gln 
    690                 695                 700                 


Arg Leu Lys Val Ser His Lys Glu Tyr Leu Glu Arg Lys Lys Leu Gly 
705                 710                 715                 720 


Lys Glu Thr Glu Thr Asp Lys Lys Leu Trp Glu Asn Phe Ile Asp Arg 
                725                 730                 735     


Gln Leu Arg Asp Thr Gln Tyr Ile Ser Arg Lys Ser Arg Glu Ile Leu 
            740                 745                 750         


Gln Lys Ile Cys Asn Asp Val Lys Thr Thr Glu Gly Gly Val Thr Ala 
        755                 760                 765             


Lys Leu Arg Asn Leu Trp Gly Trp Asp Asp Val Leu Met Asn Leu Gln 
    770                 775                 780                 


Met Pro Lys Tyr Lys Glu Leu Gly Gln Thr Ile Thr Lys Glu Trp Thr 
785                 790                 795                 800 


Ser Glu His Gly Lys Arg Lys His Gln Lys Glu Glu Ile Asp Asn Trp 
                805                 810                 815     


Thr Lys Arg Asp Asp His Arg His His Ala Ile Asp Ala Leu Val Ile 
            820                 825                 830         


Ala Cys Thr Lys Gln Gly Phe Ile Gln Arg Ile Asn Thr Leu Asn Ser 
        835                 840                 845             


Ser Asp Thr Lys Asp Leu Met Ile Lys Glu Val Glu Glu Ala Lys Ile 
    850                 855                 860                 


Glu Phe Asn Glu Lys Thr Thr Leu Leu Glu Lys Tyr Leu Lys Thr Gln 
865                 870                 875                 880 


Lys Pro Phe Ser Thr Gln Glu Val Met Lys Glu Ala Asp Lys Ile Leu 
                885                 890                 895     


Val Ser Phe Lys Ala Gly Lys Lys Val Ala Thr Ile Thr Lys Phe Lys 
            900                 905                 910         


Ala Thr Gly Lys Asn Lys Glu Thr Gly Val Ile Val Pro Arg Gly Ala 
        915                 920                 925             


Leu His Glu Gln Ser Val Tyr Gly Lys Ile Lys Val Ile Glu Lys Asp 
    930                 935                 940                 


Lys Pro Leu Lys Tyr Leu Leu Glu Asn Ser Asp Lys Ile Val Asn Pro 
945                 950                 955                 960 


Arg Ile Lys Asn Leu Ile Glu Ile Lys Leu Ser Glu Asn Lys Asn Asp 
                965                 970                 975     


Pro Lys Lys Ala Leu Leu Ala Leu Lys Lys Ile Pro Ile Phe Leu Asp 
            980                 985                 990         


Glu Glu Gln Ser Ile Thr Leu Glu  Lys Ala Ser Cys Tyr  Asn Asp Ala 
        995                 1000                 1005             


Thr Val  Leu Lys Tyr Lys Leu  Gln Asn Leu Lys Ala  Ala Gln Val 
    1010                 1015                 1020             


Asp Asp  Ile Val Asp Glu Lys  Val Lys Lys Ile Val  Arg Glu Arg 
    1025                 1030                 1035             


Leu Ser  Gln Phe Gly Asn Lys  Glu Lys Glu Ala Phe  Lys Asp Thr 
    1040                 1045                 1050             


Leu Trp  Phe Asn Glu Glu Lys  Lys Ile Pro Ile Ile  Thr Val Arg 
    1055                 1060                 1065             


Leu Phe  Ala Arg Pro Asp Ala  Asn Asn Leu Gln Thr  Ile Lys Lys 
    1070                 1075                 1080             


Asp Glu  Asn Gly Asn Gly Ile  Gly Phe Val Val Thr  Gly Asn Asn 
    1085                 1090                 1095             


His His  Ile Ala Ile Tyr Glu  Asp Lys Asp Asn Lys  Pro Val Gln 
    1100                 1105                 1110             


His Ser  Cys Thr Phe Trp His  Ala Val Glu Arg Lys  Lys Tyr Asn 
    1115                 1120                 1125             


Ile Pro  Ala Val Ile Arg Asp  Ser Ser Glu Val Trp  Asn Asp Leu 
    1130                 1135                 1140             


Leu Arg  Lys Glu Leu Pro Glu  Ser Phe Leu Asn Lys  Leu Pro Ala 
    1145                 1150                 1155             


Asp Asn  Leu Asn Leu Lys Phe  Ser Met Gln Gln Asn  Glu Met Phe 
    1160                 1165                 1170             


Ile Leu  Gly Leu Ser Gln Asp  Glu Phe Val Lys Ala  Ile Ala Asn 
    1175                 1180                 1185             


Asn Asp  Lys Ser Leu Leu Ser  Lys His Leu Tyr Leu  Val Trp Ser 
    1190                 1195                 1200             


Ile Ser  Glu Asn Asn Tyr Trp  Phe Arg His His Leu  Glu Thr Lys 
    1205                 1210                 1215             


Asn Ser  Asp Leu Lys Lys Thr  Asp Gly Val Lys Glu  Ser Lys Arg 
    1220                 1225                 1230             


Leu Tyr  Asn Ile Arg Ser Leu  Gly Ser Leu Phe Ser  Leu Asn Pro 
    1235                 1240                 1245             


Ile Lys  Val Arg Leu Asn Tyr  Leu Gly Glu Ile Thr  Lys Ile Gly 
    1250                 1255                 1260             


Glu 
    


<210>  798
<211>  1266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  798

Met Ala Lys Tyr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Ile Glu Val Asp Glu Asn Asn Ile Pro Ile Arg Ile Ile 
            20                  25                  30          


Val Met Gly Ser Arg Ile Ile Pro Leu Ser Ser Asn Asp Arg Glu Glu 
        35                  40                  45              


Phe Gln Arg Gly Leu Ser Ile Thr Lys Asn Gln Asn Arg Thr Ile Ser 
    50                  55                  60                  


Arg Thr Gln Arg Lys Gly Tyr Asp Arg Lys Gln Leu Lys Arg Asn Asp 
65                  70                  75                  80  


Leu Asn Lys Val Leu Asn Lys Tyr Asn Ile Phe Pro Ser Lys Met Leu 
                85                  90                  95      


Leu Asn Leu Pro Met Leu Asp Leu Trp Lys Leu Arg Ser Asp Ser Ala 
            100                 105                 110         


Asn Ser Asn Lys Asp Ile Ser Ala Glu Gln Leu Gly Arg Ile Leu Tyr 
        115                 120                 125             


Met Leu Asn Gln Lys Arg Gly Tyr Lys Ser Val Arg Ser Glu Ala Asn 
    130                 135                 140                 


Ser Asp Lys Lys Asp Thr Asp Tyr Val Gln Ala Val Lys Gly Arg Tyr 
145                 150                 155                 160 


Ala Gln Leu Lys Asp Arg Asn Gln Thr Ile Gly Gln Tyr Phe Tyr Glu 
                165                 170                 175     


Glu Leu Tyr Asn Ala Asn Val Asn Asn Ser Tyr Phe Lys Ile Lys Glu 
            180                 185                 190         


Glu Val Tyr Pro Arg Glu Ala Tyr Ile Glu Glu Phe Asp Thr Ile Ile 
        195                 200                 205             


Asn Ser Gln Lys Thr Lys His Glu Phe Leu Thr Asp Gln Phe Val His 
    210                 215                 220                 


Gln Leu Arg Asn Glu Ile Ile Tyr Phe Gln Arg Lys Leu Lys Ser Gln 
225                 230                 235                 240 


Lys Gly Leu Ile Ser Ile Cys Glu Phe Glu Gly Phe Glu Lys Thr Val 
                245                 250                 255     


Phe Asp Lys Glu Lys Asn Lys His Lys Ile Val Phe Val Gly Pro Lys 
            260                 265                 270         


Val Ala Pro Arg Ser Ala Pro Leu Phe Gln Leu Cys Arg Val Trp Glu 
        275                 280                 285             


Val Ile Asn Thr Ile Ser Ile Lys Val Lys Asn Pro Glu Gly Ser Lys 
    290                 295                 300                 


Tyr Lys Trp Gly Glu Arg Ile Pro Ser Ile Lys Glu Lys Glu Ile Leu 
305                 310                 315                 320 


Ala Asp Tyr Leu Phe Lys Asn Glu Val Leu Ser Phe Thr Lys Leu Leu 
                325                 330                 335     


Glu Ile Leu Gly Leu Asn Lys Asn Asp Val Tyr Val Asn Lys Gln Ile 
            340                 345                 350         


Leu Lys Gly Ile Lys Gly Asn Glu Thr Tyr Ala Thr Leu Tyr Asn Ile 
        355                 360                 365             


Leu Gly Glu Asn Glu Phe Leu Asn Phe Asp Val Ser Ile Ile Pro Ser 
    370                 375                 380                 


Asn His Thr Ser Met Leu Val Asp Lys Lys Thr Gly Glu Ile Leu Glu 
385                 390                 395                 400 


Glu Arg Ala Gly Leu Glu Leu Asp Val Ser Leu Glu Lys Gln Pro Phe 
                405                 410                 415     


Tyr Gln Leu Trp His Thr Ile Tyr Ser Leu Lys Asp Leu Asp Glu Cys 
            420                 425                 430         


Lys Asn Ala Leu Ile Lys Arg Phe Asp Phe Asp Asp Glu Thr Ala Glu 
        435                 440                 445             


Asn Leu Ser Arg Ile Asp Phe Asn Lys Gln Ala Phe Gly Asn Lys Ser 
    450                 455                 460                 


Asn Lys Ser Met Arg Lys Ile Leu Pro Phe Leu Met Glu Gly Tyr Asp 
465                 470                 475                 480 


Tyr Ser Gln Ser Cys Ser Leu Ala Gly Tyr Asn His Ser Asn Ser Leu 
                485                 490                 495     


Thr Lys Asp Glu Arg Glu Gln Gln Val Thr Leu Asp Arg Leu Glu Leu 
            500                 505                 510         


Leu Pro Lys Asn Ser Leu Arg Gln Pro Ile Val Glu Lys Ile Leu Asn 
        515                 520                 525             


Gln Met Ile Asn Leu Val Asn Ala Ile Leu Asp Gln Tyr Gly Glu Leu 
    530                 535                 540                 


Ser Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Gln Ser Lys Asp 
545                 550                 555                 560 


Glu Arg Asn Asp Ala Asp Leu Gln Asn Thr Lys Asn Lys Lys Leu Asn 
                565                 570                 575     


Asp Glu Ile Gly Lys Arg Leu Ala Glu Leu Gly Leu Pro Ile Thr Lys 
            580                 585                 590         


Arg Tyr Ile Gln Lys Tyr Lys Phe Ile Phe Pro Ser Leu Ser Lys Asn 
        595                 600                 605             


Leu Lys Asp Ala His Val Asn Asn Gln Cys Ile Tyr Cys Gly Glu Thr 
    610                 615                 620                 


Phe Asn Leu Ser Glu Ala Leu Ser Gly Asp Ala Tyr Asp Val Asp His 
625                 630                 635                 640 


Ile Val Pro Lys Ala Leu Leu Phe Asp Asp Ser Gln Thr Asn Lys Val 
                645                 650                 655     


Leu Val His Arg Lys Cys Asn Ser Thr Lys Thr Asn Gln Thr Ala Tyr 
            660                 665                 670         


Asp Tyr Ile Ser Thr Lys Gly Asp Glu Gln Val Arg Ile Tyr Phe Glu 
        675                 680                 685             


Arg Val Asp Asp Trp Phe Lys Ser Gly Ile Ile Ser Tyr Ser Lys Met 
    690                 695                 700                 


Gln Arg Leu Lys Val Ser His Lys Glu Tyr Leu Glu Arg Lys Lys Ile 
705                 710                 715                 720 


Gly Lys Glu Thr Glu Thr Asp Lys Lys Leu Trp Glu Asn Phe Ile Asp 
                725                 730                 735     


Arg Gln Leu Arg Asp Thr Gln Tyr Ile Ser Arg Lys Ser Arg Glu Ile 
            740                 745                 750         


Leu Gln Lys Val Cys Asn Asn Val Thr Thr Thr Glu Gly Gly Val Thr 
        755                 760                 765             


Ala Lys Leu Arg Asn Leu Trp Gly Trp Asp Asp Val Leu Met Asn Leu 
    770                 775                 780                 


Gln Met Pro Lys Tyr Arg Glu Leu Gly Gln Thr Val Ile Lys Glu Trp 
785                 790                 795                 800 


Thr Ser Glu His Gly Lys Arg Lys His Gln Lys Glu Glu Val Glu Asn 
                805                 810                 815     


Trp Thr Lys Arg Asp Asp His Arg His His Ala Ile Asp Ala Leu Val 
            820                 825                 830         


Ile Ala Cys Thr Lys Gln Gly Phe Ile Gln Arg Ile Asn Thr Leu Asn 
        835                 840                 845             


Ser Ser Asp Thr Lys Asp Leu Met Leu Lys Glu Val Glu Glu Ala Lys 
    850                 855                 860                 


Val Lys Phe Ser Glu Lys Thr Thr Leu Leu Glu Lys Tyr Leu Arg Thr 
865                 870                 875                 880 


Gln Glu Pro Phe Ser Thr Gln Glu Val Met Arg Glu Ala Asp Lys Val 
                885                 890                 895     


Leu Val Ser Phe Lys Ala Gly Lys Lys Val Ala Thr Ile Thr Lys Phe 
            900                 905                 910         


Lys Ala Ile Gly Lys Asn Lys Asp Ser Gly Val Ile Val Pro Arg Gly 
        915                 920                 925             


Ala Leu His Glu Gln Ser Val Tyr Gly Lys Ile Lys Val Ile Glu Lys 
    930                 935                 940                 


Asp Lys Pro Leu Lys Tyr Leu Phe Glu Asn Ser Asp Arg Ile Val Asp 
945                 950                 955                 960 


Pro Lys Val Lys Lys Leu Val Glu Val Arg Leu Ser Glu Asn Glu Asn 
                965                 970                 975     


Asn Ser Lys Thr Ala Ile Ser Thr Leu Lys Lys Ala Pro Ile Phe Leu 
            980                 985                 990         


Asn Glu Glu Lys Thr Glu Val Leu  Glu Lys Ala Ser Cys  Tyr Asn Asp 
        995                 1000                 1005             


Ala Thr  Val Leu Lys Tyr Lys  Leu Gln Ser Ile Lys  Ala Ser Gln 
    1010                 1015                 1020             


Ala Asp  Asp Ile Val Asp Glu  Arg Val Lys Phe Leu  Val Lys Glu 
    1025                 1030                 1035             


Arg Leu  Ala Gln Phe Gly Asn  Lys Glu Lys Glu Ala  Phe Lys Asp 
    1040                 1045                 1050             


Ile Leu  Trp Phe Asn Glu Glu  Lys Gln Ile Pro Ile  Ile Ser Val 
    1055                 1060                 1065             


Arg Leu  Phe Ala Arg Pro Asp  Ala Asn Asn Leu Gln  Val Ile Lys 
    1070                 1075                 1080             


Lys Asp  Glu Asn Gly Lys Ser  Ile Gly Phe Val Leu  Thr Gly Asn 
    1085                 1090                 1095             


Asn His  His Ile Ala Ile Tyr  Glu Asp Lys Asp Asn  Lys Leu Ile 
    1100                 1105                 1110             


Gln His  Ser Cys Thr Phe Trp  His Ala Val Glu Arg  Lys Lys Tyr 
    1115                 1120                 1125             


Asn Ile  Pro Val Val Ile Lys  Asn Ser Ser Asp Val  Trp Ser Gln 
    1130                 1135                 1140             


Leu Leu  Asp Lys Gln Leu Pro  Glu Ser Leu Leu Asn  Lys Leu Pro 
    1145                 1150                 1155             


Thr Asp  Lys Leu Asn Leu Lys  Phe Ser Met Gln Gln  Asn Glu Met 
    1160                 1165                 1170             


Phe Ile  Leu Gly Leu Ser Gln  Lys Glu Phe Asp Glu  Ala Ile Lys 
    1175                 1180                 1185             


Asn Asn  His Lys Ser Phe Leu  Ser Lys Tyr Leu Tyr  Leu Val Trp 
    1190                 1195                 1200             


Ser Ile  Ser Asn Asn Asp Tyr  Tyr Phe Arg His His  Leu Glu Thr 
    1205                 1210                 1215             


Lys Asn  Thr Glu Leu Lys Asn  Ile Ser Thr Ala Lys  Glu Ser Lys 
    1220                 1225                 1230             


Arg Tyr  Phe Arg Phe Lys Ser  Val Gly Ala Leu Val  Gly Thr Asn 
    1235                 1240                 1245             


Pro Ile  Lys Ile Arg Leu Asn  His Leu Gly Glu Ile  Asn Lys Val 
    1250                 1255                 1260             


Gly Glu  Tyr 
    1265     


<210>  799
<211>  1268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  799

Met Thr Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Ile Glu Val Asp Glu Asn Asn Thr Pro Ile Arg Ile Ile 
            20                  25                  30          


Ala Met Gly Ser Arg Ile Ile Pro Leu Ser Thr Ser Asp Lys Glu Glu 
        35                  40                  45              


Phe Gln Arg Gly Leu Ser Ile Thr Lys Asn Gln Ser Arg Thr Val Ser 
    50                  55                  60                  


Arg Thr Gln Arg Lys Gly Tyr Asp Arg Lys Gln Leu Lys Lys Ser Asp 
65                  70                  75                  80  


Leu Lys Lys Ile Leu Asn Lys Tyr Asn Ile Phe Pro Ser Glu Glu Leu 
                85                  90                  95      


Leu Lys Leu Pro Met Leu Asp Leu Trp Lys Leu Arg Ser Asp Ser Ala 
            100                 105                 110         


Asn Pro Asn Glu Asn Ile Ser Ala Glu Gln Leu Gly Arg Ile Leu Tyr 
        115                 120                 125             


Met Leu Asn Gln Lys Arg Gly Tyr Lys Ser Ala Arg Ser Glu Ala Asn 
    130                 135                 140                 


Ala Asp Lys Lys Asp Thr Asp Tyr Val Gln Ala Val Lys Gly Arg Tyr 
145                 150                 155                 160 


Ala Gln Leu Lys Glu Arg Asn Gln Thr Ile Gly Gln Tyr Phe Tyr Glu 
                165                 170                 175     


Glu Leu Tyr Asn Ala Asn Thr Asn Asn Ser Tyr Phe Arg Ile Lys Glu 
            180                 185                 190         


Glu Val Tyr Pro Arg Glu Ala Tyr Thr Glu Glu Phe Asp Thr Ile Ile 
        195                 200                 205             


Asn Ala Gln Lys Asn Lys His Asp Phe Leu Thr Asp Glu Val Val His 
    210                 215                 220                 


Gln Leu Arg Asn Glu Ile Ile Tyr Phe Gln Arg Lys Leu Lys Ser Gln 
225                 230                 235                 240 


Lys Gly Leu Val Ser Ile Cys Glu Phe Glu Gly Phe Glu Lys Thr Val 
                245                 250                 255     


Phe Asp Lys Glu Lys Asn Lys Asp Lys Val Val Phe Val Gly Pro Lys 
            260                 265                 270         


Val Ala Pro Lys Thr Ser Pro Leu Phe Gln Leu Cys Arg Ile Trp Glu 
        275                 280                 285             


Val Val Asn Thr Ile Ser Leu Lys Ile Lys Asn Pro Glu Gly Ser Lys 
    290                 295                 300                 


Tyr Lys Trp Gly Glu Lys Ile Pro Asn Leu Lys Glu Lys Gln Ile Leu 
305                 310                 315                 320 


Ala Asp Tyr Leu Phe Lys Asn Asp Ser Leu Ser Phe Thr Lys Leu Leu 
                325                 330                 335     


Glu Ile Leu Asp Leu Asn Lys Asn Asp Val Tyr Val Asn Lys Gln Ile 
            340                 345                 350         


Leu Lys Gly Ile Lys Gly Asn Glu Thr Phe Ser Thr Leu His Thr Val 
        355                 360                 365             


Leu Gly Asp Asn Glu Leu Leu Asn Phe Asp Val Ser Ile Ile Pro Ser 
    370                 375                 380                 


Asn His Thr Ser Met Leu Val Asp Lys Lys Thr Gly Glu Ile Leu Glu 
385                 390                 395                 400 


Glu Arg Ala Gly Leu Glu Leu Asp Ala Ser Leu Glu Lys Glu Pro Leu 
                405                 410                 415     


Tyr Gln Leu Trp His Thr Ile Tyr Ser Leu Lys Asp Leu Asp Glu Cys 
            420                 425                 430         


Lys Asn Ala Leu Ile Lys Arg Phe Gly Phe Asp Asp Glu Thr Ala Glu 
        435                 440                 445             


Lys Leu Ser His Ile Asp Phe Asn Lys Gln Ala Phe Gly Asn Lys Ser 
    450                 455                 460                 


Asn Lys Ser Met Arg Lys Ile Leu Pro Phe Leu Met Glu Gly Tyr Asp 
465                 470                 475                 480 


Tyr Ser Gln Ser Cys Ser Leu Ala Gly Tyr Asn His Ser Asn Ser Leu 
                485                 490                 495     


Thr Lys Asp Glu Arg Glu Gln Gln Ile Thr Leu Asp Arg Leu Glu Leu 
            500                 505                 510         


Leu Ser Lys Asn Ser Leu Arg Gln Pro Ile Val Glu Lys Ile Leu Asn 
        515                 520                 525             


Gln Met Ile Asn Val Val Asn Ala Ile Leu Glu Gln Tyr Gly Lys Leu 
    530                 535                 540                 


Ser Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Gln Ser Lys Asp 
545                 550                 555                 560 


Glu Arg Asn Asp Ala Asp Leu Gln Asn Ser Lys Asn Lys Lys Leu Asn 
                565                 570                 575     


Glu Glu Ile Gly Lys Arg Leu Ala Glu Leu Gly Leu Pro Val Thr Lys 
            580                 585                 590         


Arg Tyr Ile Gln Lys Tyr Lys Phe Ile Phe Pro Ser Leu Ser Lys Asn 
        595                 600                 605             


Leu Lys Asp Ala His Val Asn Asn Gln Cys Ile Tyr Cys Gly Glu Thr 
    610                 615                 620                 


Phe Asn Leu Thr Glu Ala Leu Ser Gly Asp Ala Phe Asp Val Asp His 
625                 630                 635                 640 


Ile Val Pro Lys Ala Leu Leu Phe Asp Asp Ser Gln Thr Asn Lys Val 
                645                 650                 655     


Leu Val His Arg Lys Cys Asn Ser Thr Lys Thr Asn Gln Thr Ala Tyr 
            660                 665                 670         


Asp Tyr Ile Gly Thr Lys Gly Glu Glu Gln Val Arg Val Tyr Ser Glu 
        675                 680                 685             


Arg Ile Asp Asp Trp Phe Lys Arg Gly Ile Ile Ser Tyr Ser Lys Met 
    690                 695                 700                 


Gln Arg Leu Lys Val Ser His Lys Glu Tyr Leu Glu Arg Lys Lys Leu 
705                 710                 715                 720 


Gly Lys Glu Thr Glu Thr Asp Lys Lys Leu Trp Glu Asn Phe Ile Asp 
                725                 730                 735     


Arg Gln Leu Arg Asp Thr Gln Tyr Ile Ser Arg Lys Ala Arg Glu Ile 
            740                 745                 750         


Leu Gln Lys Val Cys Asn Asn Val Thr Thr Thr Glu Gly Gly Val Thr 
        755                 760                 765             


Ala Lys Leu Arg Asn Leu Trp Gly Trp Asp Asp Val Leu Met Asn Leu 
    770                 775                 780                 


Gln Met Pro Lys Tyr Lys Glu Leu Gly Gln Thr Val Ile Lys Glu Trp 
785                 790                 795                 800 


Thr Ser Glu His Gly Lys Arg Lys His Gln Lys Glu Glu Ile Glu Asn 
                805                 810                 815     


Trp Thr Lys Arg Asp Asp His Arg His His Ala Ile Asp Ala Leu Val 
            820                 825                 830         


Val Ala Cys Thr Lys Gln Gly Phe Ile Gln Arg Ile Asn Thr Leu Asn 
        835                 840                 845             


Ser Ser Asp Thr Lys Asp Leu Met Gln Arg Glu Ile Lys Glu Ala Lys 
    850                 855                 860                 


Lys Leu Leu Gly Glu Asn Tyr Asn Glu Lys Glu Phe Glu Asn Asp Asn 
865                 870                 875                 880 


Glu Phe Val Lys Asn His Lys Glu Arg Leu Ser Gln Leu Asp Asp Tyr 
                885                 890                 895     


Leu Arg Leu Gln Lys Pro Val Lys Phe Asp Thr Lys Tyr Val Glu Gln 
            900                 905                 910         


Glu Ala Asp Lys Val Leu Val Ser Phe Lys Ala Gly Lys Lys Val Ala 
        915                 920                 925             


Thr Ile Thr Lys Phe Lys Ala Thr Gly Lys Asn Lys Asp Thr Gly Val 
    930                 935                 940                 


Ile Val Pro Arg Gly Ala Leu His Glu Ala Gly Leu Tyr Gly Ile Arg 
945                 950                 955                 960 


Thr Ile Tyr Ser Lys Gly Gly Ile Ala Glu Lys His Ile Ile Lys Lys 
                965                 970                 975     


Tyr Lys Val Gly Val Ser Ala Gln Asp His Ile Phe Thr Gly Lys Glu 
            980                 985                 990         


Thr Tyr Ser Val Lys Lys Asp Lys  Asn Gly Asn Glu Lys  Val Asp Asp 
        995                 1000                 1005             


Lys Ile  Lys Ile Val Leu Asp  Ser Ile Val Asp Asn  Lys Val Lys 
    1010                 1015                 1020             


Glu Val  Val Leu Asn Arg Leu  Asn Arg Gly Phe Asn  Asn Glu Glu 
    1025                 1030                 1035             


Asp Tyr  Arg Ile Asp Val Lys  Lys Ala Leu Asn Asn  Phe Lys Asn 
    1040                 1045                 1050             


Leu Asp  Glu Asp Pro Leu Trp  Tyr Asn Glu Ser Lys  Gly Ile Lys 
    1055                 1060                 1065             


Ile Leu  Ser Ile Lys Leu Lys  Thr Gly Leu Lys Asp  Ser Val Val 
    1070                 1075                 1080             


Pro Leu  Arg Leu Asn Glu Lys  Asn Glu Val Ile Thr  Leu Val Lys 
    1085                 1090                 1095             


Thr Gly  Asn Asn His His Ile  Ala Ile Tyr Glu Asp  Asn Asp Gly 
    1100                 1105                 1110             


Lys Leu  Ile Gln Tyr Ser Cys  Thr Phe Trp His Ala  Val Glu Arg 
    1115                 1120                 1125             


Lys Lys  Tyr Asn Ile Pro Thr  Val Ile Lys Asn Ser  Ser Glu Val 
    1130                 1135                 1140             


Trp Asn  Gln Val Leu Asp Lys  Lys Leu Pro Glu Ser  Phe Leu Asn 
    1145                 1150                 1155             


Arg Leu  Pro Ala Asp Lys Leu  Asn Leu Lys Phe Ser  Met Gln Gln 
    1160                 1165                 1170             


Asn Glu  Met Phe Ile Leu Gly  Leu Leu Glu Glu Glu  Phe Asp Lys 
    1175                 1180                 1185             


Ala Ile  Lys Asp Asn Asp Lys  Ser Leu Leu Ser Lys  Tyr Leu Tyr 
    1190                 1195                 1200             


Leu Val  Trp Ser Ile Ala Glu  Gly Asp Tyr Trp Phe  Arg His His 
    1205                 1210                 1215             


Leu Glu  Thr Lys Asn Thr Glu  Leu Lys Lys Thr Asn  Gly Ala Lys 
    1220                 1225                 1230             


Glu Ser  Lys Arg Tyr Phe Arg  Leu Ser Thr Lys Gly  Leu Ile Asp 
    1235                 1240                 1245             


Leu Asn  Pro Val Lys Val Arg  Leu Asn His Leu Gly  Glu Ile Thr 
    1250                 1255                 1260             


Lys Ile  Gly Glu Tyr 
    1265             


<210>  800
<211>  1269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  800

Met Asn Asn Ser Ile Lys Ser Lys Pro Glu Val Thr Ile Gly Leu Asp 
1               5                   10                  15      


Leu Gly Val Gly Ser Val Gly Trp Ala Ile Val Asp Asn Glu Thr Asn 
            20                  25                  30          


Ile Ile His His Leu Gly Ser Arg Leu Phe Ser Gln Ala Lys Thr Ala 
        35                  40                  45              


Glu Asp Arg Arg Ser Phe Arg Gly Ala Arg Arg Leu Ile Arg Arg Arg 
    50                  55                  60                  


Lys Tyr Lys Leu Lys Arg Phe Ile Asn Leu Ile Trp Lys Tyr Asn Ser 
65                  70                  75                  80  


Tyr Phe Gly Phe Lys Asn Lys Glu Asp Ile Leu Asn Asn Tyr Gln Glu 
                85                  90                  95      


Gln Gln Lys Leu His Asn Thr Val Leu Asn Leu Lys Leu Glu Ala Leu 
            100                 105                 110         


Asn Ala Lys Ile Asp Pro Lys Ala Leu Ser Trp Ile Leu His Asp Tyr 
        115                 120                 125             


Leu Lys Asn Arg Gly His Phe Tyr Glu Asp Asn Arg Asp Phe Asn Val 
    130                 135                 140                 


Tyr Pro Thr Glu Glu Leu Ala Asn Tyr Phe Gly Glu Tyr Gly Tyr Tyr 
145                 150                 155                 160 


Lys Gly Ile Ile Asp Ser Lys Glu Asp Asn Asp Asp Lys Leu Glu Glu 
                165                 170                 175     


Gly Leu Thr Lys Tyr Lys Phe Ser Asn Gln His Trp Leu Glu Glu Ile 
            180                 185                 190         


Lys Lys Val Leu Ser Asn Gln Thr Gly Leu Pro Glu Lys Phe Lys Glu 
        195                 200                 205             


Glu Tyr Glu Ser Leu Phe Ser Tyr Val Arg Asn Tyr Ser Glu Gly Pro 
    210                 215                 220                 


Gly Ser Ile Asn Ser Val Ser Pro Tyr Gly Ile Tyr His Leu Asp Glu 
225                 230                 235                 240 


Lys Glu Gly Lys Val Val Gln Lys Tyr Asn Asn Ile Trp Asp Lys Thr 
                245                 250                 255     


Ile Gly Lys Cys Ser Ile Phe Pro Asp Glu Tyr Arg Ala Pro Lys Asn 
            260                 265                 270         


Ser Pro Ile Ala Met Ile Phe Asn Glu Ile Asn Glu Leu Ser Thr Ile 
        275                 280                 285             


Arg Ser Tyr Ser Ile Tyr Leu Thr Gly Trp Phe Ile Asn Gln Glu Phe 
    290                 295                 300                 


Lys Lys Ala Tyr Leu Asn Lys Leu Leu Asp Leu Leu Ile Lys Thr Asn 
305                 310                 315                 320 


Ser Glu Lys Pro Ile Asp Ala Arg Gln Phe Lys Lys Leu Arg Glu Glu 
                325                 330                 335     


Thr Ile Ala Glu Ser Ile Gly Lys Glu Thr Leu Lys Asp Val Glu Arg 
            340                 345                 350         


Glu Glu Lys Leu Glu Lys Glu Asp His Lys Trp Lys Leu Lys Gly Leu 
        355                 360                 365             


Lys Leu Asn Thr Asn Gly Lys Ile Gln Tyr Asn Asp Leu Ser Ser Leu 
    370                 375                 380                 


Ala Lys Phe Val His Lys Leu Lys Gln His Leu Lys Leu Asp Phe Leu 
385                 390                 395                 400 


Leu Glu Asp Gln Tyr Thr Thr Leu Asp Lys Ile Asn Phe Leu Gln Ser 
                405                 410                 415     


Leu Phe Val Tyr Leu Gly Lys His Leu Arg Tyr Ser Asn Arg Val Asp 
            420                 425                 430         


Ser Ala Asn Leu Lys Glu Phe Ser Asp Ser Asn Lys Leu Phe Glu Arg 
        435                 440                 445             


Val Leu Gln Glu Gln Lys Asp Asp Leu Phe Lys Leu Phe Glu Gln Thr 
    450                 455                 460                 


Asp Lys Asp Asp Glu Lys Ile Leu Ala Gln Thr His Ser Leu Ser Thr 
465                 470                 475                 480 


Lys Ala Met Leu Leu Ala Ile Thr Arg Met Thr Asn Leu Asp Asn Asp 
                485                 490                 495     


Glu Asp Asn Gln Lys Asn Asn Asp Lys Gly Trp Asn Phe Glu Ala Ile 
            500                 505                 510         


Lys Asn Phe Asp Gln Lys Phe Ile Asp Ile Thr Lys Lys Asn Asn Asn 
        515                 520                 525             


Leu Ser Leu Lys Gln Asp Lys Arg Tyr Leu Asp Asp Arg Phe Ile Asn 
    530                 535                 540                 


Asp Ala Ile Leu Ser Pro Gly Val Lys Arg Ile Leu Arg Glu Ala Thr 
545                 550                 555                 560 


Lys Val Phe Asn Ala Ile Leu Lys Gln Phe Ser Glu Glu Tyr Asp Val 
                565                 570                 575     


Thr Lys Val Val Ile Glu Leu Ala Arg Glu Leu Ser Glu Glu Lys Glu 
            580                 585                 590         


Leu Glu Asn Thr Lys Asn Tyr Glu Lys Leu Ile Lys Lys Asn Ser Asp 
        595                 600                 605             


Lys Ile Ser Glu Arg Leu Glu Ala Leu Asp Ile Ala Glu Asp Lys Ile 
    610                 615                 620                 


Glu Asp Ile Leu Lys Ser Pro Thr Lys Ser Tyr Lys Val Leu Leu Trp 
625                 630                 635                 640 


Leu Gln Gln Asp His Ile Asp Pro Tyr Ser Gln Lys Glu Ile Ala Phe 
                645                 650                 655     


Glu Asp Ile Leu Thr Lys Thr Glu Lys Thr Glu Ile Asp His Ile Ile 
            660                 665                 670         


Pro Tyr Ser Ile Ser Phe Asp Asp Ser Ser Ser Asn Lys Leu Leu Val 
        675                 680                 685             


Leu Ala Glu Ser Asn Gln Ala Lys Ser Asn Gln Thr Pro Tyr Glu Phe 
    690                 695                 700                 


Ile Thr Ser Gly Asn Ala Gly Ile Lys Trp Glu Asp Tyr Glu Ala Tyr 
705                 710                 715                 720 


Cys Arg Lys Phe Lys Asp Gly Asp Thr Ser Leu Leu Asp Ser Thr Gln 
                725                 730                 735     


Arg Ser Lys Lys Phe Ala Lys Met Met Lys Thr Asp Thr Ser Ser Lys 
            740                 745                 750         


Tyr Asp Ile Gly Phe Leu Ala Arg Asn Leu Asn Asp Thr Arg Tyr Ala 
        755                 760                 765             


Thr Ile Val Phe Arg Asp Ala Leu Lys Asp Tyr Ala Asn Asn His Leu 
    770                 775                 780                 


Val Glu Asp Lys Pro Met Phe Lys Val Val Cys Ile Asn Gly Gly Val 
785                 790                 795                 800 


Thr Ser Phe Leu Arg Lys Asn Phe Asp Lys Ser Trp Tyr Ala Lys Lys 
                805                 810                 815     


Asp Arg Asp Lys Asn Ile His His Ala Val Asp Ala Ser Ile Ile Ser 
            820                 825                 830         


Ile Phe Ser Asn Glu Thr Lys Thr Leu Phe Asn Gln Leu Thr Gln Phe 
        835                 840                 845             


Ala Asp Tyr Lys Leu Phe Lys Asn Thr Asp Gly Ser Trp Lys Lys Ile 
    850                 855                 860                 


Asp Pro Lys Thr Gly Val Val Thr Glu Val Thr Asp Glu Asn Trp Lys 
865                 870                 875                 880 


Gln Ile Arg Val Arg Asn Gln Val Ser Glu Ile Ala Lys Val Ile Asn 
                885                 890                 895     


Lys Cys Ile Gln Asp Ser Asn Ile Glu Arg Lys Ala Arg Tyr Ser Arg 
            900                 905                 910         


Lys Ile Glu Asn Lys Thr Asn Ile Ser Leu Phe Asn Asp Thr Val Tyr 
        915                 920                 925             


Ser Ala Lys Lys Val Gly Tyr Glu Asp Gln Ile Lys Arg Lys Asn Leu 
    930                 935                 940                 


Lys Thr Leu Asp Ile His Glu Ser Ala Glu Glu Asn Lys Asn Ser Lys 
945                 950                 955                 960 


Val Lys Lys Gln Phe Val Tyr Arg Lys Leu Val Asn Val Ser Leu Leu 
                965                 970                 975     


Asn Asn Asp Lys Leu Ala Asp Leu Phe Ala Glu Lys Glu Asp Ile Leu 
            980                 985                 990         


Met Tyr Arg Ala Asn Pro Trp Val  Ile Asn Leu Ala Glu  Gln Ile Phe 
        995                 1000                 1005             


Asn Glu  Tyr Thr Glu Asn Lys  Lys Ile Lys Ser Gln  Asn Val Phe 
    1010                 1015                 1020             


Glu Lys  Tyr Met Leu Asp Leu  Thr Lys Glu Phe Pro  Glu Lys Phe 
    1025                 1030                 1035             


Ser Glu  Ala Phe Val Lys Ser  Met Leu Arg Asn Lys  Thr Ala Ile 
    1040                 1045                 1050             


Ile Tyr  Asn Val Glu Lys Lys  Val Val His Arg Ile  Lys Arg Leu 
    1055                 1060                 1065             


Lys Ile  Leu Ser Ser Glu Leu  Lys Glu Asn Lys Leu  Ser Asn Val 
    1070                 1075                 1080             


Ile Ile  Arg Ser Lys Asn Glu  Ser Gly Thr Lys Leu  Ser Tyr Gln 
    1085                 1090                 1095             


Asp Thr  Ile Asn Ser Ile Ala  Leu Met Ile Met Arg  Ser Ile Asp 
    1100                 1105                 1110             


Pro Thr  Ala Lys Lys Gln Tyr  Ile Arg Val Pro Leu  Asn Thr Leu 
    1115                 1120                 1125             


Asn Leu  His Leu Gly Asp Gln  Asp Phe Asp Leu His  Asn Ile Asp 
    1130                 1135                 1140             


Ala Tyr  Leu Lys Lys Pro Lys  Phe Val Lys Tyr Leu  Lys Ala Asn 
    1145                 1150                 1155             


Glu Ile  Gly Asp Glu Tyr Lys  Pro Trp Arg Val Leu  Thr Ser Gly 
    1160                 1165                 1170             


Thr Leu  Leu Ile His Lys Lys  Asp Lys Lys Leu Met  Tyr Ile Ser 
    1175                 1180                 1185             


Ser Phe  Gln Asn Leu Lys Asp  Val Ile Glu Ile Lys  Asn Leu Ile 
    1190                 1195                 1200             


Glu Thr  Glu Tyr Lys Glu Asn  Asp Asp Ser Asp Pro  Lys Lys Lys 
    1205                 1210                 1215             


Lys Lys  Ala Asn Arg Phe Leu  Met Thr Leu Ser Thr  Ile Leu Asn 
    1220                 1225                 1230             


Asp Tyr  Ile Leu Leu Asp Ala  Lys Asp Asn Phe Asp  Ile Leu Gly 
    1235                 1240                 1245             


Leu Ser  Lys Asn Arg Ile Asp  Glu Ile Leu Asn Ser  Lys Leu Gly 
    1250                 1255                 1260             


Leu Asp  Lys Ile Thr Lys 
    1265                 


<210>  801
<211>  1269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  801

Met Asn Asn Ser Ile Lys Ser Lys Pro Glu Val Thr Ile Gly Leu Asp 
1               5                   10                  15      


Leu Gly Val Gly Ser Val Gly Trp Ala Ile Val Asp Asn Glu Thr Asn 
            20                  25                  30          


Ile Ile His His Leu Gly Ser Arg Leu Phe Ser Gln Ala Lys Thr Ala 
        35                  40                  45              


Glu Asp Arg Arg Ser Phe Arg Gly Val Arg Arg Leu Ile Arg Arg Arg 
    50                  55                  60                  


Lys Tyr Lys Leu Lys Arg Phe Val Asn Leu Ile Trp Lys Tyr Asn Ser 
65                  70                  75                  80  


Tyr Phe Gly Phe Lys Asn Lys Glu Asp Ile Leu Asn Asn Tyr Gln Glu 
                85                  90                  95      


Gln Gln Lys Leu His Asn Thr Val Leu Asn Leu Lys Ser Glu Ala Leu 
            100                 105                 110         


Asn Ala Lys Ile Asp Pro Lys Ala Leu Ser Trp Ile Leu His Asp Tyr 
        115                 120                 125             


Leu Lys Asn Arg Gly His Phe Tyr Glu Asp Asn Arg Asp Phe Asn Val 
    130                 135                 140                 


Tyr Pro Thr Lys Glu Leu Ala Lys Tyr Phe Asp Lys Tyr Gly Tyr Tyr 
145                 150                 155                 160 


Lys Gly Ile Ile Asp Ser Lys Glu Asp Asn Asp Asn Lys Leu Glu Glu 
                165                 170                 175     


Glu Leu Thr Lys Tyr Lys Phe Ser Asn Lys His Trp Leu Glu Glu Val 
            180                 185                 190         


Lys Lys Val Leu Ser Asn Gln Thr Gly Leu Pro Glu Lys Phe Lys Glu 
        195                 200                 205             


Glu Tyr Glu Ser Leu Phe Ser Tyr Val Arg Asn Tyr Ser Glu Gly Pro 
    210                 215                 220                 


Gly Ser Ile Asn Ser Val Ser Pro Tyr Gly Ile Tyr His Leu Asp Glu 
225                 230                 235                 240 


Lys Glu Gly Lys Val Val Gln Lys Tyr Asn Asn Ile Trp Asp Lys Thr 
                245                 250                 255     


Ile Gly Lys Cys Asn Ile Phe Pro Asp Glu Tyr Arg Ala Pro Lys Asn 
            260                 265                 270         


Ser Pro Ile Ala Met Ile Phe Asn Glu Ile Asn Glu Leu Ser Thr Ile 
        275                 280                 285             


Arg Ser Tyr Ser Ile Tyr Leu Thr Gly Trp Phe Ile Asn Gln Glu Phe 
    290                 295                 300                 


Lys Lys Ala Tyr Leu Asn Lys Leu Leu Asp Leu Leu Ile Lys Thr Asn 
305                 310                 315                 320 


Gly Glu Lys Pro Ile Asp Ala Arg Gln Phe Lys Lys Leu Arg Glu Glu 
                325                 330                 335     


Thr Ile Ala Glu Ser Ile Gly Lys Glu Thr Leu Lys Asp Val Glu Asn 
            340                 345                 350         


Glu Glu Lys Leu Glu Lys Glu Asp His Lys Trp Lys Leu Lys Gly Leu 
        355                 360                 365             


Lys Leu Asn Thr Asn Gly Lys Ile Gln Tyr Asn Asp Leu Ser Ser Leu 
    370                 375                 380                 


Ala Lys Phe Val His Lys Leu Lys Gln His Leu Lys Leu Asp Phe Leu 
385                 390                 395                 400 


Leu Glu Asp Gln Tyr Ala Thr Leu Asp Lys Ile Asn Phe Leu Gln Ser 
                405                 410                 415     


Leu Phe Val Tyr Leu Gly Lys His Leu Arg Tyr Asn Asn Arg Val Asp 
            420                 425                 430         


Ser Ala Asn Leu Lys Glu Phe Ser Asp Ser Asn Arg Leu Phe Glu Arg 
        435                 440                 445             


Val Leu Gln Glu Gln Lys Asp Gly Leu Phe Lys Leu Phe Glu Gln Thr 
    450                 455                 460                 


Asp Lys Asp Asp Glu Lys Ile Leu Ala Gln Thr His Ser Leu Ser Thr 
465                 470                 475                 480 


Lys Ala Met Leu Leu Ala Ile Thr Arg Met Thr Asn Leu Asp Asn Asp 
                485                 490                 495     


Glu Asp Asn Gln Lys Asn Asn Asp Lys Gly Trp Asn Phe Glu Ala Ile 
            500                 505                 510         


Lys Asn Phe Asp Gln Lys Phe Ile Asp Ile Thr Lys Thr Asn Asn Asn 
        515                 520                 525             


Leu Ser Leu Lys Gln Asp Lys Arg Tyr Leu Asp Asp Arg Phe Ile Asn 
    530                 535                 540                 


Asp Ala Ile Leu Ser Pro Gly Val Lys Arg Ile Leu Arg Glu Ala Thr 
545                 550                 555                 560 


Lys Val Phe Asn Ala Ile Leu Lys Gln Phe Ser Gln Glu Tyr Asp Val 
                565                 570                 575     


Thr Lys Val Val Ile Glu Leu Ala Arg Glu Leu Ser Glu Glu Lys Glu 
            580                 585                 590         


Leu Glu Asn Asn Lys Asn Tyr Lys Lys Leu Ile Lys Lys Asn Ser Asp 
        595                 600                 605             


Lys Ile Ser Glu Gly Leu Lys Ala Leu Asp Ile Ala Glu Asp Lys Ile 
    610                 615                 620                 


Glu Asp Ile Leu Lys Ser Pro Thr Lys Ser Tyr Lys Val Leu Leu Trp 
625                 630                 635                 640 


Leu Gln Gln Asp His Ile Asp Pro Tyr Ser Gln Lys Glu Ile Ala Phe 
                645                 650                 655     


Glu Asp Ile Leu Thr Lys Thr Glu Lys Thr Glu Ile Asp His Ile Ile 
            660                 665                 670         


Pro Tyr Ser Ile Ser Phe Asp Asp Ser Ser Ser Asn Lys Leu Leu Val 
        675                 680                 685             


Leu Ala Glu Ser Asn Gln Ala Lys Ser Asn Gln Thr Pro Tyr Glu Phe 
    690                 695                 700                 


Ile Thr Ser Gly Asn Ala Gly Ile Lys Trp Glu Asp Tyr Glu Ala Tyr 
705                 710                 715                 720 


Cys Arg Lys Phe Lys Asp Gly Asp Thr Ser Leu Leu Asp Ser Thr Gln 
                725                 730                 735     


Arg Ser Lys Lys Phe Ala Lys Met Met Lys Thr Asp Thr Ser Ser Lys 
            740                 745                 750         


Tyr Asp Ile Gly Phe Leu Ala Arg Asn Leu Asn Asp Thr Arg Tyr Ala 
        755                 760                 765             


Thr Ile Val Phe Arg Asp Ala Leu Lys Asp Tyr Ala Asn Asn His Leu 
    770                 775                 780                 


Val Glu Asp Lys Pro Met Phe Lys Val Val Cys Ile Asn Gly Gly Val 
785                 790                 795                 800 


Thr Ser Phe Leu Arg Lys Asn Phe Asp Lys Ser Trp Tyr Ala Lys Lys 
                805                 810                 815     


Asp Arg Asp Lys Asn Ile His His Ala Val Asp Ala Ser Ile Ile Ser 
            820                 825                 830         


Ile Phe Ser Asn Lys Thr Lys Thr Leu Phe Asp Gln Leu Thr Gln Phe 
        835                 840                 845             


Ala Asp Tyr Lys Leu Phe Lys Asn Thr Asp Gly Ser Trp Lys Lys Ile 
    850                 855                 860                 


Asp Pro Lys Thr Gly Val Val Thr Glu Val Thr Asp Glu Asn Trp Lys 
865                 870                 875                 880 


Gln Ile Arg Val Arg Asn Gln Val Ser Lys Ile Ala Glu Glu Ile Asp 
                885                 890                 895     


Lys Cys Ile Gln Asp Ser Asn Ile Glu Arg Lys Ala Arg Tyr Ser Arg 
            900                 905                 910         


Lys Ile Glu Asn Lys Thr Asn Ile Ser Leu Phe Asn Asp Thr Val Tyr 
        915                 920                 925             


Ser Ala Lys Lys Val Gly Tyr Asp Asp Gln Ile Lys Arg Lys Asn Leu 
    930                 935                 940                 


Lys Thr Leu Asp Ile Asp Glu Ser Val Glu Glu Asn Lys Asn Ser Lys 
945                 950                 955                 960 


Val Lys Lys Gln Phe Val Tyr Arg Lys Leu Val Asn Val Ser Leu Leu 
                965                 970                 975     


Asn Asn Asp Lys Leu Ala Asp Leu Phe Ala Glu Lys Glu Asp Ile Leu 
            980                 985                 990         


Met Tyr Arg Ala Asn Pro Trp Val  Ile Asn Leu Ala Glu  Gln Ile Phe 
        995                 1000                 1005             


Asn Glu  Tyr Thr Glu Asn Arg  Lys Ile Lys Ser Gln  Asn Val Phe 
    1010                 1015                 1020             


Gly Lys  Tyr Met Leu Asp Leu  Thr Lys Glu Phe Pro  Glu Lys Phe 
    1025                 1030                 1035             


Ser Glu  Ala Phe Val Lys Ser  Met Leu Arg Asn Lys  Thr Ala Ile 
    1040                 1045                 1050             


Ile Tyr  Asn Val Glu Lys Lys  Val Val His Arg Ile  Lys Arg Leu 
    1055                 1060                 1065             


Lys Ile  Leu Ser Ser Glu Leu  Lys Glu Asn Lys Leu  Ser Asn Val 
    1070                 1075                 1080             


Ile Ile  Arg Ser Lys Asn Glu  Ser Gly Thr Lys Leu  Ser Tyr Gln 
    1085                 1090                 1095             


Asp Thr  Ile Asn Ser Val Ala  Leu Met Ile Met Arg  Ser Ile Asp 
    1100                 1105                 1110             


Pro Thr  Ala Lys Lys Gln Tyr  Ile Arg Val Pro Leu  Asn Thr Leu 
    1115                 1120                 1125             


Asn Leu  His Leu Gly Asp His  Asp Phe Asp Leu His  Asn Ile Asp 
    1130                 1135                 1140             


Ala Tyr  Leu Lys Lys Pro Lys  Phe Val Lys Tyr Leu  Lys Ala Asn 
    1145                 1150                 1155             


Glu Ile  Gly Asp Glu Tyr Lys  Pro Trp Arg Val Leu  Ile Ser Gly 
    1160                 1165                 1170             


Ser Leu  Leu Ile His Lys Arg  Asp Lys Lys Leu Met  Tyr Ile Ser 
    1175                 1180                 1185             


Ser Phe  Gln Asn Leu Asn Asp  Leu Ile Glu Ile Lys  Asn Leu Ile 
    1190                 1195                 1200             


Glu Thr  Glu Tyr Lys Glu Asn  Val Asp Ser Asp Pro  Lys Lys Lys 
    1205                 1210                 1215             


Lys Lys  Ala Ser Gln Ile Leu  Arg Ser Leu Ser Thr  Ile Leu Asn 
    1220                 1225                 1230             


Asp Tyr  Ile Leu Leu Asp Ala  Lys Asp Asn Phe Asp  Ile Leu Gly 
    1235                 1240                 1245             


Leu Ser  Lys Asn Arg Ile Asp  Glu Ile Leu Asn Ser  Lys Leu Asp 
    1250                 1255                 1260             


Leu Asp  Lys Ile Ala Lys 
    1265                 


<210>  802
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  802

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Val Asp Glu Asn Asn Phe Pro Ile Arg Ile Ile Ala 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Leu Ser Ser Asn Asp Lys Asp Glu Phe 
        35                  40                  45              


Gln Arg Gly Leu Ser Ile Thr Lys Asn Gln Ser Arg Thr Val Ser Arg 
    50                  55                  60                  


Thr Gln Arg Lys Gly Tyr Asp Arg Lys Gln Leu Lys Lys Ser Asp Asp 
65                  70                  75                  80  


Phe Lys Tyr Ser Leu Arg Arg Ile Leu Asn Lys Tyr Asn Ile Phe Pro 
                85                  90                  95      


Ser Glu Glu Leu Leu Lys Leu Pro Met Leu Asp Leu Trp Lys Leu Arg 
            100                 105                 110         


Ser Asp Ala Val Asn Pro Asn Glu Asn Ile Ser Ala Glu Gln Leu Gly 
        115                 120                 125             


Arg Ile Leu Tyr Met Leu Asn Gln Lys Arg Gly Tyr Lys Ser Ala Arg 
    130                 135                 140                 


Ser Glu Ala Asn Ala Asp Lys Lys Asp Thr Asp Tyr Val Leu Ala Val 
145                 150                 155                 160 


Lys Gly Arg Tyr Ala Gln Leu Lys Asp Arg Asn Gln Thr Ile Gly Gln 
                165                 170                 175     


Tyr Phe Tyr Glu Glu Leu His Asn Ala Lys Thr Thr Asn Ser Tyr Phe 
            180                 185                 190         


Arg Thr Lys Glu Glu Val Tyr Pro Arg Glu Ala Tyr Ile Glu Glu Phe 
        195                 200                 205             


Asp Thr Ile Ile Asn Ala Gln Lys Ser Lys His Asp Phe Leu Thr Asp 
    210                 215                 220                 


Glu Val Val His Gln Leu Arg Asn Glu Ile Ile Tyr Phe Gln Arg Lys 
225                 230                 235                 240 


Leu Lys Ser Gln Lys Gly Leu Val Ser Ile Cys Glu Phe Glu Gly Phe 
                245                 250                 255     


Glu Lys Thr Ile Phe Asp Lys Glu Lys Asp Lys Asn Lys Val Val Phe 
            260                 265                 270         


Val Gly Pro Lys Val Ala Pro Lys Thr Ser Pro Leu Phe Gln Leu Cys 
        275                 280                 285             


Arg Ile Trp Glu Val Val Asn Thr Ile Thr Leu Lys Ile Lys Asn Pro 
    290                 295                 300                 


Glu Gly Ser Lys Tyr Lys Trp Gly Glu Lys Ile Pro Asn Ile Lys Glu 
305                 310                 315                 320 


Lys Glu Ala Leu Ala Asp Tyr Leu Phe Lys Asn Glu Thr Leu Ser Phe 
                325                 330                 335     


Asn Lys Leu Leu Asp Ile Leu Glu Leu Lys Lys Glu Asn Val Tyr Val 
            340                 345                 350         


Asn Lys Gln Ile Ile Lys Gly Ile Lys Gly Asn Glu Thr Tyr Ser Ala 
        355                 360                 365             


Leu His Arg Ile Leu Gly Asp Asn Glu Leu Leu Lys Phe Asp Val Ser 
    370                 375                 380                 


Ile Ile Pro Ser Asn His Thr Ser Met Leu Val Asp Lys Lys Thr Gly 
385                 390                 395                 400 


Glu Ile Leu Glu Glu Arg Ala Gly Leu Glu Leu Asp Val Ser Leu Glu 
                405                 410                 415     


Lys Glu Pro Leu Tyr Gln Leu Trp His Thr Ile Tyr Ser Ile Lys Asp 
            420                 425                 430         


Leu Asp Glu Cys Lys Asn Ala Leu Ile Lys Arg Phe Gly Phe Asp Asp 
        435                 440                 445             


Glu Ile Ala Glu Lys Leu Ser His Leu Asp Phe Asn Lys Gln Ala Phe 
    450                 455                 460                 


Gly Asn Lys Ser Asn Lys Ser Met Arg Lys Ile Leu Pro Phe Leu Met 
465                 470                 475                 480 


Glu Gly Tyr Asn Tyr Ser Gln Ser Cys Thr Leu Ala Gly Tyr Asn His 
                485                 490                 495     


Ser Asn Ser Leu Thr Lys Asp Glu Arg Glu Gln Gln Val Thr Leu Asp 
            500                 505                 510         


Lys Leu Glu Leu Leu Pro Lys Asn Ser Leu Arg Gln Pro Ile Val Glu 
        515                 520                 525             


Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Ala Ile Leu Glu Glu 
    530                 535                 540                 


Tyr Gly Lys Pro Ser Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys 
545                 550                 555                 560 


Gln Ser Lys Asp Glu Arg His Asp Ala Asp Leu Gln Asn Ser Lys Asn 
                565                 570                 575     


Lys Lys Leu Asn Glu Glu Ile Gly Lys Arg Leu Thr Glu Leu Gly Leu 
            580                 585                 590         


Pro Val Thr Lys Arg Tyr Ile Gln Lys Tyr Lys Phe Ile Phe Pro Ser 
        595                 600                 605             


Leu Ser Lys Ser Leu Lys Asp Ala Gln Val Asn Asn Gln Cys Ile Tyr 
    610                 615                 620                 


Cys Gly Glu Thr Phe Asn Leu Thr Glu Ala Leu Ser Gly Asp Ala Tyr 
625                 630                 635                 640 


Asp Val Asp His Ile Val Pro Lys Ser Leu Leu Phe Asp Asp Ser Gln 
                645                 650                 655     


Thr Asn Lys Val Leu Val His Arg Lys Cys Asn Ser Thr Lys Thr Asn 
            660                 665                 670         


Gln Thr Ala Tyr Asp Tyr Ile Ala Ser Lys Gly Glu Glu Glu Val Gln 
        675                 680                 685             


Ile Tyr Ser Ala Arg Val Asp Asp Trp Phe Lys Arg Gly Ile Ile Ser 
    690                 695                 700                 


Tyr Ser Lys Met Gln Arg Leu Lys Val Ser His Lys Glu Tyr Leu Glu 
705                 710                 715                 720 


Arg Lys Ser Leu Asn Lys Glu Thr Glu Thr Asp Lys Lys Leu Trp Glu 
                725                 730                 735     


Asn Phe Ile Asp Arg Gln Leu Arg Asp Thr Gln Tyr Ile Ser Arg Lys 
            740                 745                 750         


Ser Lys Glu Ile Leu Gln Lys Val Cys Asn Asn Val Thr Thr Thr Glu 
        755                 760                 765             


Gly Gly Val Thr Ala Lys Leu Arg Asp Leu Trp Gly Trp Asp Asp Ile 
    770                 775                 780                 


Leu Met Asn Leu Gln Met Pro Lys Tyr Lys Glu Leu Arg Gln Thr Val 
785                 790                 795                 800 


Ile Lys Glu Trp Thr Ser Glu His Gly Lys Arg Lys His Gln Lys Glu 
                805                 810                 815     


Glu Ile Glu Asn Trp Thr Lys Arg Asp Asp His Arg His His Ala Ile 
            820                 825                 830         


Asp Ala Leu Val Ile Ala Cys Thr Lys Gln Gly Phe Ile Gln Arg Ile 
        835                 840                 845             


Asn Thr Leu Asn Ser Ser Glu Thr Lys Asp Leu Met Gln Gln Glu Ile 
    850                 855                 860                 


Asn Glu Ala Lys Lys Leu Leu Gly Glu Asn Tyr Lys Asp Lys Glu Phe 
865                 870                 875                 880 


Glu Asn Ser Asn Glu Leu Val Lys Asn His Lys Gly Arg Leu Ser Lys 
                885                 890                 895     


Leu Asp Asp Tyr Leu Arg Leu Gln Lys Pro Ile Lys Phe Asn Thr Lys 
            900                 905                 910         


Tyr Val Glu Lys Glu Ala Asp Lys Ile Leu Ile Ser Phe Lys Ala Gly 
        915                 920                 925             


Lys Lys Val Ala Thr Ile Thr Lys Phe Lys Ala Thr Gly Lys Asn Lys 
    930                 935                 940                 


Asp Thr Gly Val Ile Val Pro Arg Gly Ala Leu His Glu Ala Gly Leu 
945                 950                 955                 960 


Tyr Gly Val Arg Thr Ile Tyr Ser Lys Gly Gly Ile Ala Glu Lys His 
                965                 970                 975     


Ile Ile Lys Lys Tyr Lys Val Gly Val Ser Ala Gln Asp His Ile Phe 
            980                 985                 990         


Thr Gly Lys Glu Ser Tyr Ser Val  Lys Lys Asp Lys Asn  Gly Asn Glu 
        995                 1000                 1005             


Lys Val  Asp Asp Lys Ile Lys  Val Val Leu Asp Ser  Ile Val Asp 
    1010                 1015                 1020             


Ser Lys  Val Lys Glu Ile Val  Leu Asn Arg Leu Asn  Phe Gly Phe 
    1025                 1030                 1035             


Ser Asp  Gly Glu Asp Tyr Lys  Asn Asn Val Lys Lys  Ala Leu Asn 
    1040                 1045                 1050             


Asn Phe  Lys Asn Leu Asp Glu  Tyr Pro Leu Trp Tyr  Asn Glu Asn 
    1055                 1060                 1065             


Lys Gly  Ile Lys Ile Leu Ser  Ile Lys Leu Lys Thr  Gly Leu Lys 
    1070                 1075                 1080             


Asp Ser  Val Val Pro Leu Arg  Leu Asn Glu Ser Asn  Glu Val Ile 
    1085                 1090                 1095             


Thr Leu  Val Lys Thr Gly Asn  Asn His His Ile Ala  Ile Tyr Glu 
    1100                 1105                 1110             


Asp Lys  Asn Ser Lys Pro Val  Gln His Ser Cys Thr  Phe Trp His 
    1115                 1120                 1125             


Ala Val  Glu Arg Lys Lys Tyr  Asn Ile Pro Ala Val  Ile Lys Lys 
    1130                 1135                 1140             


Ser Ser  Asp Val Trp Asn Gln  Leu Leu Asp Lys Glu  Leu Pro Glu 
    1145                 1150                 1155             


Ser Phe  Leu Asn Asn Leu Pro  Glu Asp Asn Leu Asn  Leu Lys Phe 
    1160                 1165                 1170             


Ser Met  Gln Gln Asn Glu Met  Phe Val Leu Gly Leu  Ser Gln Glu 
    1175                 1180                 1185             


Glu Phe  Lys Glu Ala Ile Glu  Asn Asn Asp Lys Ser  Leu Leu Ser 
    1190                 1195                 1200             


Lys Tyr  Leu Tyr Leu Val Trp  Ser Val Ser Glu Asn  Asn Tyr Trp 
    1205                 1210                 1215             


Phe Arg  His His Leu Glu Thr  Lys Asn Ser Asp Leu  Lys Lys Thr 
    1220                 1225                 1230             


Glu Gly  Ala Lys Glu Ser Lys  Arg Leu Tyr Asn Ile  Arg Ser Val 
    1235                 1240                 1245             


Ser Ser  Leu Phe Ser Leu Asn  Pro Val Lys Val Arg  Leu Asn His 
    1250                 1255                 1260             


Leu Gly  Glu Ile Thr Lys Ile  Gly Glu Tyr 
    1265                 1270             


<210>  803
<211>  1274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  803

Met Thr Lys His Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Ile Ile Gln Val Asp Asn Asn Asn Asn Val Pro Ile Gln Ile 
            20                  25                  30          


Ile Ala Met Gly Ser Arg Ile Ile Pro Leu Asp Ser Asn Asp Arg Asp 
        35                  40                  45              


Gln Phe Gln Lys Gly Gln Ala Ile Ser Lys Asn Lys Asp Arg Thr Thr 
    50                  55                  60                  


Ala Arg Thr Gln Arg Lys Gly Tyr Asp Arg Lys Gln Leu Lys Lys Ser 
65                  70                  75                  80  


Asp Asp Phe Lys Tyr Ser Leu Lys Lys Ile Leu Glu Lys Leu Asp Ile 
                85                  90                  95      


Phe Pro Thr Glu Glu Leu Met Lys Leu Pro Thr Leu Asp Leu Trp Lys 
            100                 105                 110         


Leu Arg Ser Asp Ala Val Ser Asn Ile Glu Asp Ile Thr Pro Lys Gln 
        115                 120                 125             


Leu Gly Arg Ile Leu Tyr Met Leu Asn Gln Lys Arg Gly Tyr Lys Ser 
    130                 135                 140                 


Ala Arg Ser Glu Ala Asn Ala Asp Lys Lys Asp Thr Asp Tyr Val Ala 
145                 150                 155                 160 


Glu Val Lys Gly Arg Tyr Thr Gln Leu Lys Asp Lys Gly Gln Thr Leu 
                165                 170                 175     


Gly Gln Tyr Phe Tyr Lys Glu Leu Ser Asp Ala Asn Gln Asn Asn Thr 
            180                 185                 190         


Tyr Tyr Arg Val Lys Glu Lys Val Tyr Pro Arg Glu Ala Tyr Ile Glu 
        195                 200                 205             


Glu Phe Asp Ala Ile Ile Asn Val Gln Lys Ser Lys His Ser Phe Leu 
    210                 215                 220                 


Thr Asp Glu Val Ile His Ser Leu Arg Asn Glu Ile Ile Tyr Tyr Gln 
225                 230                 235                 240 


Arg Lys Leu Lys Ser Gln Lys Gly Leu Val Ser Ile Cys Glu Phe Glu 
                245                 250                 255     


Gly Phe Glu Thr Thr Tyr Phe Asp Lys Lys Thr Gln Gln Asp Lys Thr 
            260                 265                 270         


Ile Phe Thr Gly Pro Lys Val Ala Pro Arg Thr Ser Pro Leu Phe Gln 
        275                 280                 285             


Phe Cys Lys Ile Trp Glu Val Val Asn Asn Ile Ser Leu Lys Thr Lys 
    290                 295                 300                 


Asn Pro Glu Gly Ser Lys Tyr Lys Trp Ser Asp Arg Ile Pro Thr Ile 
305                 310                 315                 320 


Glu Glu Lys Gln Thr Ile Ala Asn Tyr Leu Gln Glu Asn Glu Asn Leu 
                325                 330                 335     


Ser Phe Ile Glu Leu Leu Lys Ile Leu Gln Leu Lys Lys Glu Gln Val 
            340                 345                 350         


Tyr Ala Asn Lys Gln Ile Leu Lys Gly Ile Gln Gly Asn Thr Thr Phe 
        355                 360                 365             


Ser Ala Ile His Lys Ile Ile Gly Asn Ser Glu His Leu Lys Phe Asp 
    370                 375                 380                 


Ile Glu Thr Ile Pro Ser Lys His Phe Ala Val Leu Val Asp Lys Lys 
385                 390                 395                 400 


Thr Gly Glu Ile Leu Asp Glu Arg Asp Ser Leu Glu Leu Asn Ser Ala 
                405                 410                 415     


Leu Glu Gln Glu Pro Phe Tyr Gln Leu Trp His Thr Ile Tyr Ser Ile 
            420                 425                 430         


Lys Asp Leu Asp Glu Cys Lys Lys Ala Leu Ile Lys Arg Phe Asn Phe 
        435                 440                 445             


Glu Glu Glu Ile Ala Glu Lys Leu Ser Lys Ile Asp Phe Asn Lys Gln 
    450                 455                 460                 


Ala Phe Gly Asn Lys Ser Asn Lys Ala Met Arg Lys Met Leu Pro Tyr 
465                 470                 475                 480 


Leu Met Leu Gly Tyr Asn Gln Ser Glu Ala Glu Ser Phe Ala Gly Tyr 
                485                 490                 495     


Asn Arg Arg Leu Thr Lys Glu Glu Lys Ser Lys Asn Val Ser Asp Glu 
            500                 505                 510         


Pro Leu Gln Leu Leu Ala Lys Asn Ser Leu Arg Gln Pro Val Val Glu 
        515                 520                 525             


Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Ala Ile Ile Glu Lys 
    530                 535                 540                 


Tyr Gly Lys Pro Glu Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
545                 550                 555                 560 


Gln Ser Lys Asp Glu Arg Glu Asp Ala Asp Lys Gln Asn Gly Phe Asn 
                565                 570                 575     


Lys Lys Leu Asn Glu Leu Val Ala Thr Lys Leu Thr Glu Leu Gly Leu 
            580                 585                 590         


Pro Thr Thr Lys His Tyr Ile Gln Lys Tyr Lys Phe Ile Phe Pro Ala 
        595                 600                 605             


Lys Asp Lys Asn Trp Lys Glu Ala Gln Val Ala Asn Gln Cys Ile Tyr 
    610                 615                 620                 


Cys Gly Asp Thr Phe Asn Leu Thr Glu Ala Leu Ser Gly Asp Asn Phe 
625                 630                 635                 640 


Asp Val Asp His Ile Val Pro Lys Ala Leu Leu Phe Asp Asp Ser Gln 
                645                 650                 655     


Ala Asn Lys Val Leu Val His Arg Ser Cys Asn Ser Thr Lys Thr Asn 
            660                 665                 670         


Asn Thr Ala Tyr Asp Tyr Ile Thr Lys Lys Gly Ser Gln Ala Leu Asn 
        675                 680                 685             


Asp Tyr Val Ala Arg Val Asp Asp Trp Phe Lys Arg Gly Ile Ile Ser 
    690                 695                 700                 


Tyr Gly Lys Met Gln Arg Leu Lys Val Ser Phe Glu Glu Tyr Gln Glu 
705                 710                 715                 720 


Arg Lys Lys Ile Gly Lys Glu Thr Glu Ala Asp Lys Arg Ile Trp Glu 
                725                 730                 735     


Asn Phe Ile Asp Arg Gln Leu Arg Glu Thr Ala Tyr Ile Ala Lys Lys 
            740                 745                 750         


Ala Lys Glu Ile Leu Glu Lys Val Cys His Asn Val Thr Ser Thr Glu 
        755                 760                 765             


Gly Asn Val Thr Ala Lys Leu Arg Gln Leu Trp Gly Trp Asp Asn Val 
    770                 775                 780                 


Leu Met Asn Leu Gln Leu Pro Lys Tyr Lys Glu Leu Glu Lys Lys Thr 
785                 790                 795                 800 


Lys Gln Thr Phe Thr Gln Leu Lys Glu Trp Thr Ser Asp His Gly Asn 
                805                 810                 815     


Arg Lys His Gln Lys Glu Glu Ile Ile Asn Trp Thr Lys Arg Asp Asp 
            820                 825                 830         


His Arg His His Ala Ile Asp Ala Leu Val Ile Ala Cys Thr Gln Gln 
        835                 840                 845             


Gly Phe Ile Gln Arg Ile Asn Thr Leu Ser Ser Ser Asp Val Lys Asp 
    850                 855                 860                 


Glu Met Lys Lys Glu Leu Glu Glu Asp Lys Thr Val Tyr Asn Glu Arg 
865                 870                 875                 880 


Leu Thr Leu Leu Glu Asn Tyr Leu Leu Glu Lys Lys Pro Phe Ser Thr 
                885                 890                 895     


Glu Glu Ile Glu Lys Glu Ala Asp Lys Ile Leu Val Ser Phe Lys Ala 
            900                 905                 910         


Gly Lys Lys Val Ala Thr Leu Ser Lys Tyr Lys Ala Thr Gly Ile Asn 
        915                 920                 925             


Glu Ile Lys Gly Val Leu Val Pro Arg Gly Pro Leu His Glu Gln Ser 
    930                 935                 940                 


Val Tyr Gly Lys Ile Lys Val Ile Glu Lys Asp Lys Pro Leu Lys Tyr 
945                 950                 955                 960 


Leu Phe Glu Asn Ser Asp Lys Ile Val Asn Pro Leu Ile Lys His Leu 
                965                 970                 975     


Val Lys Thr Arg Leu Leu Glu Asn Glu Asn Asn Ala Gln Ala Ala Leu 
            980                 985                 990         


Val Thr Leu Lys Asn Lys Pro Ile  Leu Leu Asn Asn Lys  Gln Thr Glu 
        995                 1000                 1005             


Ile Leu  Glu Lys Ala Ser Cys  Tyr Asn Glu Ala Thr  Val Leu Lys 
    1010                 1015                 1020             


Tyr Lys  Leu Gln Ser Leu Lys  Ala Ser Gln Ile Asp  Asp Ile Val 
    1025                 1030                 1035             


Asp Glu  Lys Ile Lys Phe Leu  Ile Lys Glu Arg Leu  Ser Lys Phe 
    1040                 1045                 1050             


Gly Asn  Lys Glu Lys Glu Ala  Phe Lys Asp Ile Leu  Trp Phe Asn 
    1055                 1060                 1065             


Glu Lys  Lys Gln Ile Pro Ile  Thr Ser Ile Arg Leu  Phe Ala Arg 
    1070                 1075                 1080             


Pro Asp  Ala Asn Asn Leu Gln  Val Ile Lys Lys His  Glu Lys Gly 
    1085                 1090                 1095             


Lys Asn  Ile Gly Phe Val Leu  Ser Gly Asn Asn His  His Ile Ala 
    1100                 1105                 1110             


Ile Tyr  Glu Asp Lys Asn Asn  Lys Leu Ile Gln His  Ile Cys Asp 
    1115                 1120                 1125             


Phe Trp  His Ala Val Glu Arg  Lys Arg Asn Asn Ile  Pro Val Leu 
    1130                 1135                 1140             


Ile Glu  Asp Thr Ser Thr Ile  Trp Asn His Leu Ile  Asn Glu Asp 
    1145                 1150                 1155             


Phe Ser  Glu Ser Phe Leu Asn  Lys Leu Pro Asn Asp  Ser Leu Lys 
    1160                 1165                 1170             


Leu Lys  Phe Ser Leu Gln Gln  Asn Glu Met Phe Ile  Leu Gly Leu 
    1175                 1180                 1185             


Pro Lys  Glu Gln Ser Glu Glu  Ala Ile Lys Ser Asn  Asn Lys Ser 
    1190                 1195                 1200             


Leu Leu  Ser Lys His Leu Tyr  Leu Val Trp Ser Ile  Thr Asp Gly 
    1205                 1210                 1215             


Asp Tyr  Phe Phe Arg His His  Leu Glu Thr Lys Asn  Thr Glu Leu 
    1220                 1225                 1230             


Lys Lys  Ile Asp Gly Ser Lys  Glu Ser Lys Arg Tyr  Leu Arg Leu 
    1235                 1240                 1245             


Ser Thr  Lys Ser Leu Val Asp  Leu Asn Pro Ile Lys  Val Arg Leu 
    1250                 1255                 1260             


Asn His  Leu Gly Glu Ile Thr  Lys Ile Gly Glu 
    1265                 1270                 


<210>  804
<211>  1281
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  804

Met Glu Asp Asp Ser Gln His Asp Ile Arg Glu Ile Tyr Leu Ala Ile 
1               5                   10                  15      


His His Met Val Lys Tyr Arg Gly Asn Phe Leu Val Glu Gly Thr Leu 
            20                  25                  30          


Glu Ser Ser Asn Ala Phe Lys Glu Asp Glu Leu Leu Lys Leu Leu Gly 
        35                  40                  45              


Arg Ile Thr Arg Tyr Glu Met Ser Glu Gly Glu Gln Asn Ser Asp Ile 
    50                  55                  60                  


Glu Gln Asp Asp Glu Asn Lys Leu Val Ala Pro Ala Asn Gly Gln Leu 
65                  70                  75                  80  


Ala Asp Ala Leu Cys Ala Thr Arg Gly Ser Arg Ser Met Arg Val Asp 
                85                  90                  95      


Asn Ala Leu Glu Ala Leu Ser Ala Val Asn Asp Leu Ser Arg Glu Gln 
            100                 105                 110         


Arg Ala Ile Val Lys Ala Ile Phe Ala Gly Leu Glu Gly Asn Lys Leu 
        115                 120                 125             


Asp Leu Ala Lys Ile Phe Val Ser Lys Glu Phe Ser Ser Glu Asn Lys 
    130                 135                 140                 


Lys Ile Leu Gly Ile Tyr Phe Asn Lys Ser Asp Tyr Glu Glu Lys Cys 
145                 150                 155                 160 


Val Gln Ile Val Asp Ser Gly Leu Leu Asp Asp Glu Glu Arg Glu Phe 
                165                 170                 175     


Leu Asp Arg Met Gln Gly Gln Tyr Asn Ala Ile Ala Leu Lys Gln Leu 
            180                 185                 190         


Leu Gly Arg Ser Thr Ser Val Ser Asp Ser Lys Cys Ala Ser Tyr Asp 
        195                 200                 205             


Ala His Arg Ala Asn Trp Asn Leu Ile Lys Leu Gln Leu Arg Thr Lys 
    210                 215                 220                 


Glu Asn Glu Lys Asp Ile Asn Glu Asn Tyr Gly Ile Leu Val Gly Trp 
225                 230                 235                 240 


Lys Ile Asp Ser Gly Gln Arg Lys Ser Val Arg Gly Glu Ser Ala Tyr 
                245                 250                 255     


Glu Asn Met Arg Lys Lys Ala Asn Val Phe Phe Lys Lys Met Ile Glu 
            260                 265                 270         


Thr Ser Asp Leu Ser Glu Thr Asp Lys Asn Arg Leu Ile His Asp Ile 
        275                 280                 285             


Glu Glu Asp Lys Leu Phe Pro Ile Gln Arg Asp Ser Asp Asn Gly Val 
    290                 295                 300                 


Ile Pro His Gln Leu His Gln Asn Glu Leu Lys Gln Ile Ile Lys Lys 
305                 310                 315                 320 


Gln Gly Lys Tyr Tyr Pro Phe Leu Leu Asp Ala Phe Glu Lys Asp Gly 
                325                 330                 335     


Lys Gln Ile Asn Lys Ile Glu Gly Leu Leu Ala Phe Arg Val Pro Tyr 
            340                 345                 350         


Phe Val Gly Pro Leu Val Val Pro Glu Asp Leu Gln Lys Ser Asp Asn 
        355                 360                 365             


Ser Glu Asn His Trp Met Val Arg Lys Lys Lys Gly Glu Ile Thr Pro 
    370                 375                 380                 


Trp Asn Phe Asp Glu Met Val Asp Lys Asp Ala Ser Gly Arg Lys Phe 
385                 390                 395                 400 


Ile Glu Arg Leu Val Gly Thr Asp Ser Tyr Leu Leu Gly Glu Pro Thr 
                405                 410                 415     


Leu Pro Lys Asn Ser Leu Leu Tyr Gln Glu Tyr Glu Val Leu Asn Glu 
            420                 425                 430         


Leu Asn Asn Val Arg Leu Ser Val Arg Thr Gly Asn His Trp Asn Asp 
        435                 440                 445             


Lys Arg Arg Met Arg Leu Gly Arg Glu Glu Lys Thr Leu Leu Cys Gln 
    450                 455                 460                 


Arg Leu Phe Met Lys Gly Gln Thr Val Thr Lys Arg Thr Ala Glu Asn 
465                 470                 475                 480 


Leu Leu Arg Lys Glu Tyr Gly Arg Thr Tyr Glu Leu Ser Gly Leu Ser 
                485                 490                 495     


Asp Glu Ser Lys Phe Thr Ser Ser Leu Ser Thr Tyr Gly Lys Met Cys 
            500                 505                 510         


Arg Ile Phe Gly Glu Lys Tyr Val Asn Glu His Arg Asp Leu Met Glu 
        515                 520                 525             


Lys Ile Val Glu Leu Gln Thr Val Phe Glu Asp Lys Glu Thr Leu Leu 
    530                 535                 540                 


His Gln Leu Arg Gln Leu Glu Gly Ile Ser Glu Ala Asp Cys Ala Leu 
545                 550                 555                 560 


Leu Val Asn Thr His Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
                565                 570                 575     


Leu Thr Thr Lys Ala Gly Glu Cys Lys Ile Ser Asp Asp Phe Ala Pro 
            580                 585                 590         


Arg Lys His Ser Ile Ile Glu Ile Met Arg Ala Glu Asp Arg Asn Leu 
        595                 600                 605             


Met Glu Ile Ile Thr Asp Lys Gln Leu Gly Phe Ser Asp Trp Ile Glu 
    610                 615                 620                 


Gln Glu Asn Leu Gly Ala Glu Asn Gly Ser Ser Leu Met Glu Val Val 
625                 630                 635                 640 


Asp Asp Leu Arg Val Ser Pro Lys Val Lys Arg Gly Ile Ile Gln Ser 
                645                 650                 655     


Ile Arg Leu Ile Asp Asp Ile Ser Lys Ala Val Gly Lys Arg Pro Ser 
            660                 665                 670         


Arg Ile Phe Leu Glu Leu Ala Asp Asp Ile Gln Pro Ser Gly Arg Thr 
        675                 680                 685             


Ile Ser Arg Lys Ser Arg Leu Gln Asp Leu Tyr Arg Asn Ala Asn Leu 
    690                 695                 700                 


Gly Lys Glu Phe Lys Gly Ile Ala Asp Glu Leu Asn Ala Cys Ser Asp 
705                 710                 715                 720 


Lys Asp Leu Gln Asp Asp Arg Leu Phe Leu Tyr Tyr Thr Gln Leu Gly 
                725                 730                 735     


Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Leu Asp Arg Leu Ser Ser 
            740                 745                 750         


Ala Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Val Thr Gln Asn Asp 
        755                 760                 765             


Ser Ile Asp Asn Arg Val Leu Val Ala Arg Ala Glu Asn Ala Arg Lys 
    770                 775                 780                 


Thr Asp Ser Phe Thr Tyr Met Pro Gln Ile Ala Asp Arg Met Arg Asn 
785                 790                 795                 800 


Phe Trp Gln Ile Leu Leu Asp Asn Gly Leu Ile Ser Arg Val Lys Phe 
                805                 810                 815     


Glu Arg Leu Thr Arg Gln Asn Glu Phe Ser Glu Arg Glu Lys Glu Arg 
            820                 825                 830         


Phe Val Gln Arg Ser Leu Val Glu Thr Arg Gln Ile Met Lys Asn Val 
        835                 840                 845             


Ala Thr Leu Met Arg Gln Arg Tyr Gly Asn Ser Ala Ala Val Ile Gly 
    850                 855                 860                 


Leu Asn Ala Glu Leu Thr Lys Glu Met His Arg Tyr Leu Gly Phe Ser 
865                 870                 875                 880 


His Lys Asn Arg Asp Ile Asn Asp Tyr His His Ala Gln Asp Ala Leu 
                885                 890                 895     


Cys Val Gly Ile Ala Gly Gln Phe Ala Ala Asn Arg Gly Phe Phe Ala 
            900                 905                 910         


Asp Gly Glu Val Ser Asp Gly Ala Gln Asn Ser Tyr Asn Gln Tyr Leu 
        915                 920                 925             


Arg Asp Tyr Leu Arg Gly Tyr Arg Glu Lys Leu Ser Ala Glu Asp Arg 
    930                 935                 940                 


Lys Gln Gly Arg Ala Phe Gly Phe Ile Val Gly Ser Met Arg Ser Gln 
945                 950                 955                 960 


Asp Glu Gln Lys Arg Val Asn Pro Arg Thr Gly Glu Val Val Trp Ser 
                965                 970                 975     


Glu Glu Asp Lys Asp Tyr Leu Arg Lys Val Met Asn Tyr Arg Lys Met 
            980                 985                 990         


Leu Val Thr Gln Lys Val Gly Asp  Asp Phe Gly Ala Leu  Tyr Asp Glu 
        995                 1000                 1005             


Thr Arg  Tyr Ala Ala Thr Asp  Pro Lys Gly Ile Lys  Gly Ile Pro 
    1010                 1015                 1020             


Phe Asp  Gly Ala Lys Gln Asp  Thr Ser Leu Tyr Gly  Gly Phe Ser 
    1025                 1030                 1035             


Ser Ala  Lys Pro Ala Tyr Ala  Val Leu Ile Glu Ser  Lys Gly Lys 
    1040                 1045                 1050             


Thr Arg  Leu Val Asn Val Thr  Met Gln Glu Tyr Ser  Leu Leu Gly 
    1055                 1060                 1065             


Asp Arg  Pro Ser Asp Asp Glu  Leu Arg Lys Val Leu  Ala Lys Lys 
    1070                 1075                 1080             


Lys Ser  Glu Tyr Ala Lys Ala  Asn Ile Leu Leu Arg  His Val Pro 
    1085                 1090                 1095             


Lys Met  Gln Leu Ile Arg Tyr  Gly Gly Gly Leu Met  Val Ile Lys 
    1100                 1105                 1110             


Ser Ala  Gly Glu Leu Asn Asn  Ala Gln Gln Leu Trp  Leu Pro Tyr 
    1115                 1120                 1125             


Glu Glu  Tyr Cys Tyr Phe Asp  Asp Leu Ser Gln Gly  Lys Gly Ser 
    1130                 1135                 1140             


Leu Glu  Lys Asp Asp Leu Lys  Lys Leu Leu Asp Ser  Ile Leu Gly 
    1145                 1150                 1155             


Ser Val  Gln Cys Leu Tyr Pro  Trp His Arg Phe Thr  Glu Glu Glu 
    1160                 1165                 1170             


Leu Ala  Asp Leu His Val Ala  Phe Asp Lys Leu Pro  Glu Asp Glu 
    1175                 1180                 1185             


Lys Lys  Asn Val Ile Thr Gly  Ile Val Ser Ala Leu  His Ala Asp 
    1190                 1195                 1200             


Ala Lys  Thr Ala Asn Leu Ser  Ile Val Gly Met Thr  Gly Ser Trp 
    1205                 1210                 1215             


Arg Arg  Met Asn Asn Lys Ser  Gly Tyr Thr Phe Ser  Asp Glu Asp 
    1220                 1225                 1230             


Glu Phe  Ile Phe Gln Ser Pro  Ser Gly Leu Phe Glu  Lys Arg Val 
    1235                 1240                 1245             


Thr Val  Gly Glu Leu Lys Arg  Lys Ala Lys Lys Glu  Val Asn Ser 
    1250                 1255                 1260             


Lys Tyr  Arg Thr Asn Glu Lys  Arg Leu Pro Thr Leu  Ser Gly Ala 
    1265                 1270                 1275             


Ser Gln  Pro 
    1280     


<210>  805
<211>  1286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  805

Met Thr Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Ile Glu Val Asp Glu Asn Asn Thr Pro Ile Arg Ile Ile 
            20                  25                  30          


Ala Met Gly Ser Arg Ile Ile Pro Leu Ser Thr Ser Asp Lys Glu Glu 
        35                  40                  45              


Phe Gln Arg Gly Leu Ser Ile Thr Lys Asn Gln Ser Arg Thr Val Ser 
    50                  55                  60                  


Arg Thr Gln Arg Lys Gly Tyr Asp Arg Lys Gln Leu Lys Lys Ser Asp 
65                  70                  75                  80  


Leu Lys Lys Ile Leu Asn Lys Tyr Asn Ile Phe Pro Ser Glu Glu Leu 
                85                  90                  95      


Leu Lys Leu Pro Met Leu Asp Leu Trp Lys Leu Arg Ser Asp Ser Ala 
            100                 105                 110         


Asn Pro Asn Glu Asn Ile Ser Ala Glu Gln Leu Gly Arg Ile Leu Tyr 
        115                 120                 125             


Met Leu Asn Gln Lys Arg Gly Tyr Lys Ser Ala Arg Ser Glu Ala Asn 
    130                 135                 140                 


Ala Asp Lys Lys Asp Thr Asp Tyr Val Gln Ala Val Lys Gly Arg Tyr 
145                 150                 155                 160 


Ala Gln Leu Lys Glu Arg Asn Gln Thr Ile Gly Gln Tyr Phe Tyr Glu 
                165                 170                 175     


Glu Leu Tyr Asn Ala Asn Thr Asn Asn Ser Tyr Phe Arg Ile Lys Glu 
            180                 185                 190         


Glu Val Tyr Pro Arg Glu Ala Tyr Thr Glu Glu Phe Asp Thr Ile Ile 
        195                 200                 205             


Asn Ala Gln Lys Asn Lys His Asp Phe Leu Thr Asp Glu Val Val His 
    210                 215                 220                 


Gln Leu Arg Asn Glu Ile Ile Tyr Phe Gln Arg Lys Leu Lys Ser Gln 
225                 230                 235                 240 


Lys Gly Leu Val Ser Ile Cys Glu Phe Glu Gly Phe Glu Lys Thr Val 
                245                 250                 255     


Phe Asp Lys Glu Lys Asn Lys Asp Lys Val Val Phe Val Gly Ser Lys 
            260                 265                 270         


Val Ala Pro Lys Thr Ser Pro Leu Phe Gln Leu Cys Arg Ile Trp Glu 
        275                 280                 285             


Val Val Asn Thr Ile Ser Leu Lys Ile Lys Asn Pro Glu Gly Ser Lys 
    290                 295                 300                 


Tyr Lys Trp Gly Glu Lys Thr Pro Asn Leu Lys Glu Lys Glu Ile Leu 
305                 310                 315                 320 


Ala Asp Tyr Leu Phe Lys Asn Asp Ser Leu Ser Phe Thr Lys Leu Leu 
                325                 330                 335     


Glu Ile Leu Asp Leu Asn Lys Asn Asp Val Tyr Val Asn Lys Gln Ile 
            340                 345                 350         


Leu Lys Gly Ile Lys Gly Asn Glu Thr Phe Ser Ala Leu His Thr Val 
        355                 360                 365             


Leu Gly Asp Asn Glu Leu Leu Asn Phe Asp Val Ser Ile Ile Pro Ser 
    370                 375                 380                 


Asn His Thr Ser Met Leu Val Asp Lys Lys Thr Gly Glu Ile Leu Glu 
385                 390                 395                 400 


Glu Arg Ala Gly Leu Glu Leu Asp Ala Ser Leu Glu Lys Glu Pro Leu 
                405                 410                 415     


Tyr Gln Leu Trp His Thr Ile Tyr Ser Leu Lys Val Leu Asp Glu Cys 
            420                 425                 430         


Lys Asn Ala Leu Ile Lys Arg Phe Gly Phe Asp Asp Glu Thr Ala Glu 
        435                 440                 445             


Lys Leu Ser His Ile Asp Phe Asn Lys Gln Ala Phe Gly Asn Lys Ser 
    450                 455                 460                 


Asn Lys Ser Met Arg Lys Ile Leu Pro Phe Leu Met Glu Gly Tyr Asp 
465                 470                 475                 480 


Tyr Ser Gln Ser Cys Ser Leu Ala Gly Tyr Asn His Ser Asn Ser Leu 
                485                 490                 495     


Thr Lys Asp Glu Arg Glu Gln Gln Ile Thr Leu Asp Arg Leu Glu Leu 
            500                 505                 510         


Leu Ser Lys Asn Ser Leu Arg Gln Pro Ile Val Glu Lys Ile Leu Asn 
        515                 520                 525             


Gln Met Ile Asn Val Val Asn Ala Ile Leu Glu Gln Tyr Gly Lys Leu 
    530                 535                 540                 


Ser Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Gln Ser Lys Asp 
545                 550                 555                 560 


Glu Arg Asn Asp Ala Asp Leu Gln Asn Ser Lys Asn Lys Lys Leu Asn 
                565                 570                 575     


Glu Glu Ile Gly Lys Arg Leu Ala Glu Leu Gly Leu Pro Val Thr Lys 
            580                 585                 590         


Arg Tyr Ile Gln Lys Tyr Lys Phe Ile Phe Pro Ser Leu Ser Lys Asn 
        595                 600                 605             


Leu Lys Asp Ala His Val Asn Asn Gln Cys Ile Tyr Cys Gly Glu Thr 
    610                 615                 620                 


Phe Asn Leu Thr Glu Ala Leu Ser Gly Asp Ala Phe Asp Val Asp His 
625                 630                 635                 640 


Ile Val Pro Lys Ala Leu Leu Phe Asp Asp Ser Gln Thr Asn Lys Val 
                645                 650                 655     


Leu Val His Arg Lys Cys Asn Ser Thr Lys Thr Asn Gln Thr Ala Tyr 
            660                 665                 670         


Asp Tyr Ile Gly Thr Lys Gly Glu Glu Gln Val Arg Ile Tyr Ser Glu 
        675                 680                 685             


Arg Val Asp Asp Trp Phe Lys Arg Gly Ile Ile Ser Tyr Ser Lys Met 
    690                 695                 700                 


Gln Arg Leu Lys Val Ser His Lys Glu Tyr Leu Glu Arg Lys Lys Leu 
705                 710                 715                 720 


Gly Lys Glu Thr Glu Thr Asp Lys Lys Leu Trp Glu Asn Phe Ile Asp 
                725                 730                 735     


Arg Gln Leu Arg Asp Thr Gln Tyr Ile Ser Arg Lys Ala Arg Glu Ile 
            740                 745                 750         


Leu Gln Lys Val Cys Asn Asn Val Thr Thr Thr Glu Gly Gly Ile Thr 
        755                 760                 765             


Ala Lys Leu Arg Asn Leu Trp Gly Trp Asp Asp Val Leu Met Asn Leu 
    770                 775                 780                 


Gln Met Pro Lys Tyr Lys Glu Leu Gly Gln Thr Val Ile Lys Glu Trp 
785                 790                 795                 800 


Thr Ser Glu His Gly Lys Arg Lys His Gln Lys Glu Glu Ile Glu Asn 
                805                 810                 815     


Trp Thr Lys Arg Asp Asp His Arg His His Ala Ile Asp Ala Leu Val 
            820                 825                 830         


Val Ala Cys Thr Lys Gln Gly Phe Ile Gln Arg Ile Asn Thr Leu Asn 
        835                 840                 845             


Ser Ser Asp Thr Lys Asp Leu Met Gln Arg Glu Ile Lys Glu Ala Lys 
    850                 855                 860                 


Lys Leu Leu Gly Glu Asn Tyr Asn Glu Lys Glu Phe Glu Asn Asp Asn 
865                 870                 875                 880 


Glu Phe Val Lys Asn His Lys Glu Arg Leu Ser Gln Leu Asp Asp Tyr 
                885                 890                 895     


Leu Arg Leu Gln Lys Pro Val Lys Phe Asp Thr Lys Tyr Val Glu Gln 
            900                 905                 910         


Glu Ala Asp Lys Val Leu Val Ser Phe Lys Ala Gly Lys Lys Val Ala 
        915                 920                 925             


Thr Ile Thr Lys Phe Lys Ala Thr Gly Lys Asn Lys Asp Thr Gly Val 
    930                 935                 940                 


Ile Val Leu Arg Gly Ala Leu His Glu Gln Ser Val Tyr Gly Lys Ile 
945                 950                 955                 960 


Lys Val Ile Glu Lys Asp Lys Pro Leu Lys Tyr Leu Phe Glu Asn Ser 
                965                 970                 975     


Lys Lys Ile Val Asn Pro Lys Ile Lys Val Leu Val Glu Glu Arg Leu 
            980                 985                 990         


Ala Glu Asn Glu Asn Asn Ser Lys  Leu Ser Leu Ser Thr  Leu Lys Lys 
        995                 1000                 1005             


Asn Pro  Ile Phe Leu Asn Glu  Glu Lys Thr Glu Ile  Leu Glu Lys 
    1010                 1015                 1020             


Ala Ser  Cys Tyr Asn Asp Ala  Thr Val Leu Lys Tyr  Lys Leu Gln 
    1025                 1030                 1035             


Ser Leu  Lys Ala Ser Gln Ala  Asp Asp Ile Val Asp  Asp Arg Val 
    1040                 1045                 1050             


Lys Phe  Leu Val Lys Glu Arg  Leu Ala Gln Phe Gly  Asn Lys Glu 
    1055                 1060                 1065             


Lys Glu  Ala Phe Lys Asp Thr  Leu Trp Leu Asn Glu  Glu Lys Gln 
    1070                 1075                 1080             


Ile Pro  Ile Ile Thr Val Arg  Leu Phe Ala Arg Pro  Asp Ala Asn 
    1085                 1090                 1095             


Asn Leu  Gln Ala Ile Lys Lys  Asp Lys Asn Gly Arg  Asp Ile Gly 
    1100                 1105                 1110             


Phe Val  Leu Thr Gly Asn Asn  His His Ile Ala Ile  Tyr Glu Asp 
    1115                 1120                 1125             


Lys Asp  Asn Lys Leu Val Gln  His Ser Cys Thr Phe  Trp His Ala 
    1130                 1135                 1140             


Val Glu  Arg Lys Lys Asn Asn  Val Pro Ala Val Ile  Lys Asn Ser 
    1145                 1150                 1155             


Ser Asp  Val Trp Asn Gln Leu  Leu Asp Lys Glu Leu  Pro Glu Ser 
    1160                 1165                 1170             


Phe Leu  Asn Lys Leu Pro Ile  Asp Lys Leu Lys Leu  Lys Phe Ser 
    1175                 1180                 1185             


Leu Gln  Gln Asn Glu Met Phe  Val Leu Gly Leu Leu  Gln Glu Asp 
    1190                 1195                 1200             


Phe Glu  Ala Ala Ile Glu Asn  Asn Asp Lys Thr Leu  Leu Ser Lys 
    1205                 1210                 1215             


His Leu  Tyr Leu Val Trp Ser  Ile Thr Asp Gly Asp  Tyr Phe Phe 
    1220                 1225                 1230             


Arg His  His Leu Glu Thr Lys  Asn Thr Glu Leu Lys  Lys Thr Glu 
    1235                 1240                 1245             


Gly Ala  Lys Glu Ser Lys Arg  Tyr Phe Arg Leu Ser  Thr Lys Gly 
    1250                 1255                 1260             


Leu Ile  Asp Leu Asn Pro Val  Lys Val Arg Leu Asn  His Leu Gly 
    1265                 1270                 1275             


Glu Ile  Thr Lys Ile Gly Glu  Tyr 
    1280                 1285     


<210>  806
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  806

Met Lys Thr Val Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Leu Asp Glu Asn Asn Ile Pro Ile Arg Ile Val Ala 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Leu Ser Thr Ser Asp Lys Glu Glu Phe 
        35                  40                  45              


Gln Arg Gly Leu Ser Ile Thr Lys Asn Gln Ser Arg Thr Val Ser Arg 
    50                  55                  60                  


Thr Gln Arg Lys Gly Tyr Asp Arg Lys Gln Leu Lys Lys Ser Tyr Leu 
65                  70                  75                  80  


Lys Lys Ile Leu Asn Lys Tyr Asn Ile Phe Pro Ser Asp Glu Leu Leu 
                85                  90                  95      


Lys Leu Pro Met Leu Asp Leu Trp Lys Leu Arg Ser Asp Ser Ala Asn 
            100                 105                 110         


Pro Asn Glu Asn Ile Ser Ala Glu Gln Leu Gly Arg Ile Leu Tyr Met 
        115                 120                 125             


Leu Asn Gln Lys Arg Gly Tyr Lys Ser Ala Arg Ser Glu Ala Asn Ala 
    130                 135                 140                 


Asp Lys Lys Asp Thr Asp Tyr Ile Gln Ala Val Lys Gly Arg Tyr Ala 
145                 150                 155                 160 


Gln Leu Lys Asp Arg Asn Gln Thr Ile Gly Gln Tyr Phe Tyr Gly Glu 
                165                 170                 175     


Leu Asn Asp Ala Asn Glu Asn Asn Ser Tyr Phe Arg Ile Lys Glu Glu 
            180                 185                 190         


Val Tyr Pro Arg Glu Ala Tyr Thr Glu Glu Phe Asp Thr Ile Ile Asn 
        195                 200                 205             


Ala Gln Lys Asp Lys His Asp Phe Leu Thr Asp Glu Val Ile His Gln 
    210                 215                 220                 


Leu Arg Asn Glu Ile Ile Tyr Phe Gln Arg Lys Leu Lys Ser Gln Lys 
225                 230                 235                 240 


Gly Leu Val Ser Ile Cys Glu Phe Glu Gly Phe Glu Lys Thr Val Phe 
                245                 250                 255     


Asp Lys Glu Lys Asn Lys Asp Lys Val Val Phe Val Gly Pro Lys Val 
            260                 265                 270         


Ala Pro Lys Thr Ser Pro Leu Phe Gln Leu Cys Arg Ile Trp Glu Val 
        275                 280                 285             


Val Asn Thr Ile Thr Leu Lys Ile Lys Asn Pro Glu Gly Ser Lys Tyr 
    290                 295                 300                 


Lys Trp Gly Glu Lys Ile Pro Asp Val Lys Glu Lys Glu Ile Leu Ala 
305                 310                 315                 320 


Asp Tyr Leu Phe Lys Asn Glu Ser Leu Ser Phe Thr Lys Leu Leu Glu 
                325                 330                 335     


Ile Leu Lys Leu Asn Lys Asn Asn Val Tyr Phe Asn Lys Gln Ile Leu 
            340                 345                 350         


Lys Gly Ile Lys Gly Asn Glu Thr Tyr Ser Ile Leu His Ser Ile Leu 
        355                 360                 365             


Gly Asp Ser Glu Leu Leu Asn Phe Asp Val Ser Ile Ile Pro Ser Asn 
    370                 375                 380                 


His Thr Ser Met Leu Ile Asp Lys Lys Thr Gly Glu Ile Leu Glu Glu 
385                 390                 395                 400 


Arg Ala Gly Leu Glu Leu Asp Thr Ser Leu Glu Lys Glu Pro Leu Asn 
                405                 410                 415     


Gln Leu Trp His Thr Ile Tyr Ser Ile Lys Asp Leu Asp Glu Cys Lys 
            420                 425                 430         


Ser Ala Leu Ile Lys Arg Phe Gly Phe Asp Asp Glu Thr Ala Asp Lys 
        435                 440                 445             


Leu Ser Arg Ile Asp Phe Asn Lys Gln Ala Phe Gly Asn Lys Ser Asn 
    450                 455                 460                 


Lys Ser Met Arg Lys Ile Leu Pro Phe Leu Met Glu Gly Tyr Asp Tyr 
465                 470                 475                 480 


Ser Gln Ser Cys Ser Leu Ala Gly Tyr Asn His Ser Asn Ser Phe Thr 
                485                 490                 495     


Lys Asp Glu Arg Glu Gln Gln Ile Thr Leu Asp Lys Leu Glu Leu Leu 
            500                 505                 510         


Pro Lys Asn Ser Leu Arg Gln Pro Ile Val Glu Lys Ile Leu Asn Gln 
        515                 520                 525             


Met Ile Asn Val Val Asn Ala Ile Leu Glu Arg Tyr Gly Lys Pro Ser 
    530                 535                 540                 


Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Gln Ser Lys Asp Glu 
545                 550                 555                 560 


Arg Asn Asp Thr Asp Leu Gln Asn Ser Lys Asn Lys Lys Leu Asn Glu 
                565                 570                 575     


Glu Ile Gly Lys Arg Leu Ala Glu Leu Gly Leu Pro Leu Thr Lys Arg 
            580                 585                 590         


Tyr Ile Gln Lys Tyr Lys Phe Ile Phe Pro Ser Leu Ser Lys Asn Leu 
        595                 600                 605             


Lys Asp Ala His Val Asn Asn Gln Cys Ile Tyr Cys Gly Glu Thr Phe 
    610                 615                 620                 


Asn Leu Thr Glu Ala Leu Ser Gly Asp Ala Phe Asp Val Asp His Ile 
625                 630                 635                 640 


Val Pro Lys Ala Leu Leu Phe Asp Asp Ser Gln Thr Asn Lys Val Leu 
                645                 650                 655     


Val His Arg Lys Cys Asn Ser Thr Lys Thr Asn Gln Thr Ala Tyr Asp 
            660                 665                 670         


Tyr Ile Ala Thr Lys Gly Asp Glu Gln Val Arg Val Tyr Ser Glu Arg 
        675                 680                 685             


Val Asp Asp Trp Phe Lys Arg Gly Ile Ile Ser Tyr Ser Lys Met Gln 
    690                 695                 700                 


Arg Leu Lys Val Ser His Lys Glu Tyr Leu Glu Arg Lys Lys Leu Gly 
705                 710                 715                 720 


Lys Glu Thr Glu Thr Asp Lys Lys Leu Trp Glu Asn Phe Ile Asp Arg 
                725                 730                 735     


Gln Leu Arg Asp Thr Gln Tyr Ile Ser Arg Lys Ser Arg Glu Ile Leu 
            740                 745                 750         


Gln Lys Val Cys Asn Asn Val Ala Thr Thr Glu Gly Gly Val Thr Ala 
        755                 760                 765             


Lys Leu Arg Asn Leu Trp Gly Trp Asp Asp Val Leu Met Asn Leu Gln 
    770                 775                 780                 


Met Pro Lys Tyr Lys Glu Leu Gly Gln Thr Val Val Lys Glu Trp Thr 
785                 790                 795                 800 


Ser Glu His Gly Lys Arg Lys His Gln Lys Glu Glu Ile Glu Asn Trp 
                805                 810                 815     


Thr Lys Arg Asp Asp His Arg His His Ala Ile Asp Ala Leu Val Val 
            820                 825                 830         


Ala Cys Thr Lys Gln Gly Phe Ile Gln Arg Ile Asn Thr Leu Asn Ser 
        835                 840                 845             


Ser Glu Thr Lys Asp Leu Met Gln Gln Glu Ile Lys Glu Ala Lys Lys 
    850                 855                 860                 


Leu Leu Gly Glu Asn Tyr Asn Glu Lys Glu Phe Glu Asn Asn Asn Glu 
865                 870                 875                 880 


Ser Val Lys Asn His Lys Glu Arg Leu Ser Gln Leu Asp Asp Tyr Leu 
                885                 890                 895     


Arg Leu Gln Lys Pro Val Lys Phe Asp Thr Lys Tyr Val Glu Gln Glu 
            900                 905                 910         


Ala Asp Lys Val Leu Val Ser Phe Lys Ser Gly Lys Lys Val Ala Thr 
        915                 920                 925             


Ile Thr Lys Phe Lys Ala Thr Gly Lys Asn Lys Asp Thr Gly Val Ile 
    930                 935                 940                 


Val Pro Arg Gly Ala Leu His Glu Gln Asn Val Tyr Gly Lys Ile Lys 
945                 950                 955                 960 


Val Ile Glu Lys Asp Lys Ser Leu Lys Tyr Leu Phe Glu Asn Ser Glu 
                965                 970                 975     


Lys Ile Val Asn Pro Lys Ile Lys Asn Ile Val Glu Ile Ile Leu Ser 
            980                 985                 990         


Glu Asn Glu Asn Asn Ser Lys Met  Ala Leu Ser Thr Leu  Lys Lys Asn 
        995                 1000                 1005             


Pro Ile  Phe Leu Asn Glu Glu  Lys Thr Asp Leu Leu  Glu Lys Ala 
    1010                 1015                 1020             


Ser Cys  Tyr Glu Tyr Val Thr  Val Arg Lys Tyr Pro  Leu Asn Thr 
    1025                 1030                 1035             


Ile Lys  Ala Ser Gln Ala Asp  Asp Ile Val Asp Glu  Arg Val Lys 
    1040                 1045                 1050             


Phe Leu  Val Lys Glu Arg Leu  Thr Gln Phe Gly Asn  Lys Glu Lys 
    1055                 1060                 1065             


Glu Ala  Phe Lys Asp Thr Leu  Trp Phe Asn Glu Glu  Lys Gln Ile 
    1070                 1075                 1080             


Pro Ile  Ile Thr Val Arg Leu  Phe Ala Arg Pro Asp  Ala Asn Asn 
    1085                 1090                 1095             


Leu Gln  Ala Ile Lys Lys Asp  Glu Ser Gly Lys Glu  Ile Gly Phe 
    1100                 1105                 1110             


Val Val  Met Gly Asn Asn His  His Ile Ala Ile Tyr  Glu Asp Lys 
    1115                 1120                 1125             


Asp Gly  Lys Leu Val Gln His  Ser Cys Thr Phe Trp  His Ala Val 
    1130                 1135                 1140             


Glu Arg  Lys Lys Tyr Asn Ile  Ser Thr Val Ile Lys  Asn Ser Ser 
    1145                 1150                 1155             


Asp Ala  Trp Asn Gln Phe Leu  Asp Asn Glu Leu Pro  Glu Pro Phe 
    1160                 1165                 1170             


Leu Asn  Lys Leu Pro Ala Asp  Asn Leu Asn Leu Lys  Phe Ser Leu 
    1175                 1180                 1185             


Gln Gln  Asn Glu Met Phe Ile  Leu Gly Leu Ser Gln  Glu Glu Phe 
    1190                 1195                 1200             


Asp Lys  Ala Leu Lys Ser Asn  Asp Lys Ser Leu Leu  Ser Lys His 
    1205                 1210                 1215             


Leu Tyr  Leu Val Trp Ser Ile  Ser Glu Asn Asn Tyr  Trp Phe Arg 
    1220                 1225                 1230             


His His  Leu Glu Thr Lys Asn  Ser Asp Leu Lys Lys  Thr Asn Gly 
    1235                 1240                 1245             


Val Lys  Glu Ser Lys Arg Leu  Tyr Asn Ile Arg Ser  Leu Gly Ser 
    1250                 1255                 1260             


Leu Phe  Ser Leu Asn Pro Ile  Lys Ile Arg Leu Asn  His Leu Gly 
    1265                 1270                 1275             


Glu Ile  Thr Lys Ile Gly Glu  Tyr Ser 
    1280                 1285         


<210>  807
<211>  1288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  807

Met Arg Leu Phe Glu Glu Gly His Thr Ala Glu Asp Arg Arg Leu Lys 
1               5                   10                  15      


Arg Thr Ala Arg Arg Arg Ile Ser Arg Arg Arg Asn Arg Leu Arg Tyr 
            20                  25                  30          


Leu Gln Ala Phe Phe Glu Glu Ala Met Thr Asp Leu Asp Glu Asn Phe 
        35                  40                  45              


Phe Ala Arg Leu Gln Glu Ser Phe Leu Val Pro Glu Asp Lys Lys Trp 
    50                  55                  60                  


His Arg His Pro Ile Phe Ala Lys Leu Glu Asp Glu Val Ala Tyr His 
65                  70                  75                  80  


Glu Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp Ser 
                85                  90                  95      


Ser Glu Gln Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Ile 
            100                 105                 110         


Val Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Ser Thr Glu 
        115                 120                 125             


Asn Thr Ser Val Lys Asp Gln Phe Gln Gln Phe Met Val Ile Tyr Asn 
    130                 135                 140                 


Gln Thr Phe Val Asn Gly Glu Ser Arg Leu Val Ser Ala Pro Leu Pro 
145                 150                 155                 160 


Glu Ser Val Leu Ile Glu Glu Glu Leu Thr Glu Lys Ala Ser Arg Thr 
                165                 170                 175     


Lys Lys Ser Glu Lys Val Leu Gln Gln Phe Pro Gln Glu Lys Ala Asn 
            180                 185                 190         


Gly Leu Phe Gly Gln Phe Leu Lys Leu Met Val Gly Asn Lys Ala Asp 
        195                 200                 205             


Phe Lys Lys Val Phe Gly Leu Glu Glu Glu Ala Lys Ile Thr Tyr Ala 
    210                 215                 220                 


Ser Glu Ser Tyr Glu Glu Asp Leu Glu Gly Ile Leu Ala Lys Val Gly 
225                 230                 235                 240 


Asp Glu Tyr Ser Asp Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala 
                245                 250                 255     


Val Glu Leu Ser Thr Ile Leu Ala Asp Ser Asp Lys Lys Ser His Ala 
            260                 265                 270         


Lys Leu Ser Ser Ser Met Ile Val Arg Phe Thr Glu His Gln Glu Asp 
        275                 280                 285             


Leu Lys Lys Phe Lys Arg Phe Ile Arg Glu Asn Cys Pro Asp Glu Tyr 
    290                 295                 300                 


Asp Asn Leu Phe Lys Asn Glu Gln Lys Asp Gly Tyr Ala Gly Tyr Ile 
305                 310                 315                 320 


Ala His Ala Gly Lys Val Ser Gln Leu Lys Phe Tyr Gln Tyr Val Lys 
                325                 330                 335     


Lys Ile Ile Gln Asp Ile Ala Gly Ala Glu Tyr Phe Leu Glu Lys Ile 
            340                 345                 350         


Ala Gln Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Val 
        355                 360                 365             


Ile Pro His Gln Ile His Leu Ala Glu Leu Gln Ala Ile Ile His Arg 
    370                 375                 380                 


Gln Ala Ala Tyr Tyr Pro Phe Leu Lys Glu Asn Gln Glu Lys Ile Glu 
385                 390                 395                 400 


Gln Leu Val Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ser Lys 
                405                 410                 415     


Gly Asp Ala Ser Thr Phe Ala Trp Leu Lys Arg Gln Ser Glu Glu Pro 
            420                 425                 430         


Ile Arg Pro Trp Asn Leu Gln Glu Thr Val Asp Leu Asp Gln Ser Ala 
        435                 440                 445             


Thr Ala Phe Ile Glu Arg Met Thr Asn Phe Asp Thr Tyr Leu Pro Ser 
    450                 455                 460                 


Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Lys Phe Met Val 
465                 470                 475                 480 


Phe Asn Glu Leu Thr Lys Ile Ser Tyr Thr Asp Asp Arg Gly Ile Lys 
                485                 490                 495     


Ala Asn Phe Ser Gly Lys Glu Lys Glu Lys Ile Phe Asp Tyr Leu Phe 
            500                 505                 510         


Lys Thr Arg Arg Lys Val Lys Lys Lys Asp Ile Ile Gln Phe Tyr Arg 
        515                 520                 525             


Asn Glu Tyr Asn Thr Glu Ile Val Thr Leu Ser Gly Leu Glu Glu Asp 
    530                 535                 540                 


Gln Phe Asn Ala Ser Phe Ser Thr Tyr Gln Asp Leu Leu Lys Cys Gly 
545                 550                 555                 560 


Leu Thr Arg Ala Glu Leu Asp His Pro Asp Asn Ala Glu Lys Leu Glu 
                565                 570                 575     


Asp Ile Ile Lys Ile Leu Thr Ile Phe Glu Asp Arg Gln Arg Ile Arg 
            580                 585                 590         


Thr Gln Leu Ser Thr Phe Lys Gly Gln Phe Ser Ala Glu Val Leu Lys 
        595                 600                 605             


Lys Leu Glu Arg Lys His Tyr Thr Gly Trp Gly Arg Leu Ser Lys Lys 
    610                 615                 620                 


Leu Ile Asn Gly Ile Tyr Asp Lys Glu Ser Gly Lys Thr Ile Leu Asp 
625                 630                 635                 640 


Tyr Leu Val Lys Asp Asp Gly Val Ser Lys His Tyr Asn Arg Asn Phe 
                645                 650                 655     


Met Gln Leu Ile Asn Asp Ser Gln Leu Ser Phe Lys Asn Ala Ile Gln 
            660                 665                 670         


Lys Ala Gln Ser Ser Glu His Glu Glu Thr Leu Ser Glu Thr Val Asn 
        675                 680                 685             


Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Tyr Gln Ser Leu 
    690                 695                 700                 


Lys Ile Val Asp Glu Leu Val Ala Ile Met Gly Tyr Ala Pro Lys Arg 
705                 710                 715                 720 


Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr Ser Thr Gly Lys 
                725                 730                 735     


Arg Arg Ser Ile Gln Arg Leu Lys Ile Val Glu Lys Ala Met Ala Glu 
            740                 745                 750         


Ile Gly Ser Asn Leu Leu Lys Glu Gln Pro Thr Thr Asn Glu Gln Leu 
        755                 760                 765             


Arg Asp Thr Arg Leu Phe Leu Tyr Tyr Met Gln Asn Gly Lys Asp Met 
    770                 775                 780                 


Tyr Thr Gly Asp Glu Leu Ser Leu His Arg Leu Ser His Tyr Asp Ile 
785                 790                 795                 800 


Asp His Ile Ile Pro Gln Ser Phe Met Lys Asp Asp Ser Leu Asp Asn 
                805                 810                 815     


Leu Val Leu Val Gly Ser Thr Glu Asn Arg Gly Lys Ser Asp Asp Val 
            820                 825                 830         


Pro Ser Lys Glu Val Val Lys Asp Met Lys Ala Tyr Trp Glu Lys Leu 
        835                 840                 845             


Tyr Ala Ala Gly Leu Ile Ser Gln Arg Lys Phe Gln Arg Leu Thr Lys 
    850                 855                 860                 


Gly Glu Gln Gly Gly Leu Thr Leu Glu Asp Lys Ala His Phe Ile Gln 
865                 870                 875                 880 


Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn Val Ala Gly Ile 
                885                 890                 895     


Leu Asp Gln Arg Tyr Asn Ala Lys Ser Lys Glu Lys Lys Val Gln Ile 
            900                 905                 910         


Ile Thr Leu Lys Ala Ser Leu Thr Ser Gln Phe Arg Ser Ile Phe Gly 
        915                 920                 925             


Leu Tyr Lys Val Arg Glu Val Asn Asp Tyr His His Gly Gln Asp Ala 
    930                 935                 940                 


Tyr Leu Asn Cys Val Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Asn 
945                 950                 955                 960 


Leu Ala Pro Glu Phe Val Tyr Gly Glu Tyr Pro Lys Phe Gln Thr Phe 
                965                 970                 975     


Lys Glu Asn Lys Ala Thr Ala Lys Ala Ile Ile Tyr Thr Asn Leu Leu 
            980                 985                 990         


Arg Phe Phe Thr Glu Asp Glu Pro  Arg Phe Thr Lys Asp  Gly Glu Ile 
        995                 1000                 1005             


Leu Trp  Ser Asn Ser Tyr Leu  Lys Thr Ile Lys Lys  Glu Leu Asn 
    1010                 1015                 1020             


Tyr His  Gln Met Asn Ile Val  Lys Lys Val Glu Val  Gln Lys Gly 
    1025                 1030                 1035             


Gly Phe  Ser Lys Glu Ser Ile  Lys Pro Lys Gly Pro  Ser Asn Lys 
    1040                 1045                 1050             


Leu Ile  Pro Val Lys Asn Gly  Leu Asp Pro Gln Lys  Tyr Gly Gly 
    1055                 1060                 1065             


Phe Asp  Ser Pro Val Val Ala  Tyr Thr Val Leu Phe  Thr His Glu 
    1070                 1075                 1080             


Lys Gly  Lys Lys Pro Leu Ile  Lys Gln Glu Ile Leu  Gly Ile Thr 
    1085                 1090                 1095             


Ile Met  Glu Lys Thr Arg Phe  Glu Gln Asn Pro Ile  Leu Phe Leu 
    1100                 1105                 1110             


Glu Glu  Lys Gly Phe Leu Arg  Pro Arg Val Leu Met  Lys Leu Pro 
    1115                 1120                 1125             


Lys Tyr  Thr Leu Tyr Glu Phe  Pro Glu Gly Arg Arg  Arg Leu Leu 
    1130                 1135                 1140             


Ala Ser  Ala Lys Glu Ala Gln  Lys Gly Asn Gln Met  Val Leu Pro 
    1145                 1150                 1155             


Glu His  Leu Leu Thr Leu Leu  Tyr His Ala Lys Gln  Cys Leu Leu 
    1160                 1165                 1170             


Pro Asn  Gln Ser Glu Ser Leu  Ala Tyr Val Glu Gln  His Gln Pro 
    1175                 1180                 1185             


Glu Phe  Gln Glu Ile Leu Glu  Arg Val Val Asp Phe  Ala Glu Val 
    1190                 1195                 1200             


His Thr  Leu Ala Lys Ser Lys  Val Gln Gln Ile Val  Lys Leu Phe 
    1205                 1210                 1215             


Glu Ala  Asn Gln Thr Ala Asp  Val Lys Glu Ile Ala  Ala Ser Phe 
    1220                 1225                 1230             


Ile Gln  Leu Met Gln Phe Asn  Ala Met Gly Ala Pro  Ser Thr Phe 
    1235                 1240                 1245             


Lys Phe  Phe Gln Lys Asp Ile  Glu Arg Ala Arg Tyr  Thr Ser Ile 
    1250                 1255                 1260             


Lys Glu  Ile Phe Asp Ala Thr  Ile Ile Tyr Gln Ser  Pro Thr Gly 
    1265                 1270                 1275             


Leu Tyr  Glu Thr Arg Arg Lys  Val Val Asp 
    1280                 1285             


<210>  808
<211>  1300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  808

Met Trp Gly Val Ser Leu Phe Glu Ala Gly Lys Thr Ala Ala Glu Arg 
1               5                   10                  15      


Arg Gly Tyr Arg Ser Thr Arg Arg Arg Leu Asn His Arg Lys Phe Arg 
            20                  25                  30          


Leu Arg Leu Leu Glu Asp Met Phe Glu Lys Glu Ile Leu Ser Lys Asp 
        35                  40                  45              


Pro Ser Phe Phe Ile Arg Leu Lys Glu Ala Phe Leu Ser Pro Lys Asp 
    50                  55                  60                  


Glu Gln Lys Gln Phe Lys Gly Asn Ile Leu Phe Asn Asp Lys Asp Tyr 
65                  70                  75                  80  


Thr Asp Ala Asp Tyr Tyr Glu Gln Tyr Lys Thr Ile Tyr His Leu Arg 
                85                  90                  95      


Tyr Asp Leu Ile Ser Gln His Arg Gln Phe Asp Ile Arg Glu Val Tyr 
            100                 105                 110         


Leu Ala Ile His His Leu Ile Lys Tyr Arg Gly His Phe Ile Tyr Glu 
        115                 120                 125             


Asp Gln Thr Phe Thr Thr Asp Gly Asn Gln Leu Gln His His Ile Lys 
    130                 135                 140                 


Ala Ile Ile Thr Met Ile Asn Ser Thr Leu Asn Arg Ile Ile Ile Pro 
145                 150                 155                 160 


Glu Thr Ile Asp Ile Asn Val Phe Glu Lys Ile Leu Leu Asp Arg Met 
                165                 170                 175     


Met Asn Arg Ser Ser Lys Val Lys Phe Leu Ile Glu Leu Thr Gly Glu 
            180                 185                 190         


Asp Lys Gln Asp Lys Pro Leu Leu Lys Glu Leu Phe Asn Leu Ile Val 
        195                 200                 205             


Gly Leu Lys Ala Lys Pro Ala Ser Ile Phe Glu Gln Glu Asn Leu Ala 
    210                 215                 220                 


Thr Ile Val Glu Thr Met Asn Met Ser Thr Glu Gln Val Gln Leu Asp 
225                 230                 235                 240 


Leu Leu Thr Leu Ala Asp Val Leu Ala Asp Glu Glu Tyr Asp Leu Leu 
                245                 250                 255     


Leu Thr Ala Gln Lys Ile Tyr Ser Ala Ile Ile Leu Asp Glu Ser Met 
            260                 265                 270         


Asp Gly Tyr Glu Tyr Phe Ala Glu Ala Lys Lys Glu Ser Tyr Arg Lys 
        275                 280                 285             


His Gln Glu Glu Leu Val Leu Val Lys Lys Met Leu Lys Ser Asn Ala 
    290                 295                 300                 


Ile Thr Asn Asp Glu Arg Ala Lys Phe Glu Tyr Phe Tyr Thr Asp Tyr 
305                 310                 315                 320 


Ile Gly Ala Lys Ser Ser Asn Tyr Glu Glu Ser Lys Asn Ile Lys Lys 
                325                 330                 335     


Gly Leu Ser Ala Ala Tyr Gly Lys Tyr Ser Lys Glu Glu Arg Leu Phe 
            340                 345                 350         


Lys His Ile Glu Leu Leu Leu Ala Lys Glu Asn Val Leu Thr Thr Val 
        355                 360                 365             


Glu His Ala Leu Leu Glu Lys Asn Ile Thr Phe Ala Ser Leu Leu Pro 
    370                 375                 380                 


Leu Gln Arg Ser Ser Arg Asn Ala Val Ile Pro Tyr Gln Val His Glu 
385                 390                 395                 400 


Lys Glu Leu Val Ala Ile Leu Glu Asn Gln Ala Thr Tyr Tyr Pro Phe 
                405                 410                 415     


Leu Leu Glu Gln Lys Asp Asn Ile His Lys Leu Leu Thr Phe Arg Ile 
            420                 425                 430         


Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gln Lys Asp Ser Glu Phe Ala 
        435                 440                 445             


Trp Met Val Arg Lys Gln Ala Gly Lys Ile Thr Pro Phe Asn Phe Glu 
    450                 455                 460                 


Glu Met Val Asp Ile Asp Ala Ser Ser Glu Ala Phe Ile Lys Arg Met 
465                 470                 475                 480 


Thr Asn Lys Cys Thr Tyr Leu Ile His Glu Asp Val Ile Pro Lys His 
                485                 490                 495     


Ser Phe Ser Tyr Ala Lys Phe Glu Val Leu Asn Glu Leu Asn Lys Ile 
            500                 505                 510         


Arg Leu Asp Gly Lys Pro Ile Asp Ile Pro Leu Lys Lys Arg Ile Phe 
        515                 520                 525             


Glu Gly Leu Phe Leu Glu Lys Thr Lys Val Thr Gln Thr Ser Leu Lys 
    530                 535                 540                 


Lys Trp Leu Ala Glu His Glu His Met Thr Val Ser Val Val Gln Gly 
545                 550                 555                 560 


Thr Gln Lys Glu Thr Glu Phe Ala Thr Ser Leu Gln Ala Phe His Arg 
                565                 570                 575     


Phe Val Lys Ile Phe Asp Arg Glu Thr Val Ser Asn Pro Ala Asn Glu 
            580                 585                 590         


Glu Met Phe Glu Lys Ile Ile Tyr Trp Ser Thr Val Phe Glu Asp Lys 
        595                 600                 605             


Lys Ile Met Arg Arg Lys Leu Ser Glu Tyr Pro Gln Leu Thr Glu Gln 
    610                 615                 620                 


Gln Gln Val Gln Leu Ala Gln Val Arg Phe Arg Gly Trp Gly Arg Leu 
625                 630                 635                 640 


Ser Gln Arg Leu Ile Asn Arg Ile Lys Thr Pro Val Ser Gly Asp Glu 
                645                 650                 655     


Asp His Lys Leu Ser Ile Asn Glu Ile Leu Trp Gln Thr Asn Glu Asn 
            660                 665                 670         


Phe Met Gln Ile Ile Arg Asn Lys Asp Tyr Leu Phe Lys Lys Ile Ile 
        675                 680                 685             


Glu Glu Gln Phe Glu Asn Glu Thr Ala Leu Leu Asn Lys Gln Arg Ile 
    690                 695                 700                 


Asp Glu Leu Ala Ala Ser Pro Ala Asn Lys Lys Gly Ile Trp Gln Ala 
705                 710                 715                 720 


Ile Lys Ile Val Lys Glu Leu Glu Lys Val Leu Gln Gln Pro Ala Glu 
                725                 730                 735     


Asn Ile Phe Ile Glu Phe Ala Arg Ser Asp Glu Glu Ser Lys Arg Ser 
            740                 745                 750         


Thr Pro Arg Asp Lys Phe Ile Glu Lys Ala Tyr Ala Gln Leu Lys Lys 
        755                 760                 765             


Glu Thr Asp Thr Phe Asn Leu Glu His Leu Lys Glu Leu Lys Gln Arg 
    770                 775                 780                 


Ser Lys Gln Leu Ser Ser Gln Arg Leu Phe Leu Tyr Phe Ile Gln Asn 
785                 790                 795                 800 


Gly Lys Cys Met Tyr Ser Gly Glu His Leu Asp Ile Glu Arg Leu Asp 
                805                 810                 815     


Ser Tyr Glu Val Asp His Ile Leu Pro Gln Ser Tyr Ile Lys Asp Asn 
            820                 825                 830         


Ser Ile Glu Asn Leu Ala Leu Val Lys Lys Val Glu Asn Gln Arg Lys 
        835                 840                 845             


Lys Asp Ser Leu Leu Leu Asn Ser Ser Ile Ile Asn Gln Asn Tyr Ser 
    850                 855                 860                 


Arg Trp Glu Gln Leu Lys Asn Ala Gly Leu Ile Gly Glu Lys Lys Phe 
865                 870                 875                 880 


Arg Asn Leu Thr Arg Thr Lys Ile Thr Asp Arg Asp Lys Glu Gly Phe 
                885                 890                 895     


Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Thr 
            900                 905                 910         


Gln Leu Leu Gln Gln Glu Tyr Lys Asp Thr Thr Lys Val Phe Ala Ile 
        915                 920                 925             


Lys Ala Thr Leu Val Ser Gly Leu Arg Arg Lys Phe Glu Phe Ile Lys 
    930                 935                 940                 


Asn Arg Asn Val Asn Asp Tyr His His Ala Gln Asp Ala Phe Leu Val 
945                 950                 955                 960 


Ala Phe Leu Gly Thr Asn Ile Thr Ser Asn Tyr Pro Lys Ile Glu Met 
                965                 970                 975     


Glu Tyr Leu Phe Lys Gly Tyr Gln His Tyr Leu Asn Glu Val Gly Lys 
            980                 985                 990         


Ser Ala Ala Lys Pro Lys Phe Thr  Phe Ile Val Glu Asn  Leu Ser Lys 
        995                 1000                 1005             


Gln Gln  Tyr Asn Ser Thr Thr  Gly Glu Val Lys Trp  Asn Pro Glu 
    1010                 1015                 1020             


Val Asp  Ile Ala Lys Leu Lys  Arg Ile Leu Asn Phe  Lys Gln Cys 
    1025                 1030                 1035             


Asn Ile  Val Arg Lys Val Glu  Glu Gln Ser Gly Ala  Leu Phe Lys 
    1040                 1045                 1050             


Glu Thr  Ile Tyr Pro Val Glu  Glu Ser Ser Ser Lys  Thr Ile Pro 
    1055                 1060                 1065             


Leu Lys  Lys His Leu Asp Thr  Ala Ile Tyr Gly Gly  Tyr Thr Ala 
    1070                 1075                 1080             


Val Asn  Tyr Ala Ser Tyr Ala  Leu Ile Gln Phe Lys  Lys Gly Arg 
    1085                 1090                 1095             


Lys Leu  Lys Arg Glu Ile Ile  Gly Ile Pro Leu Ala  Val Gln Thr 
    1100                 1105                 1110             


Arg Ile  Asp Asn Ser Glu Thr  Ser Leu Gln Ala Tyr  Ile Ala Glu 
    1115                 1120                 1125             


Gln Ile  Lys Ser Glu Val Glu  Ile Leu Asn Gly Arg  Ile Leu Lys 
    1130                 1135                 1140             


Tyr Gln  Leu Ile Ser Asn Asn  Gly Asn Arg Leu Tyr  Ile Ala Gly 
    1145                 1150                 1155             


Pro Ser  Glu Arg His Asn Ala  Arg Gln Leu Ile Val  Ser Asp Glu 
    1160                 1165                 1170             


Ala Ala  Lys Val Ile Trp Leu  Ile Ser Thr Lys Gln  Ala Asp Glu 
    1175                 1180                 1185             


Ala Met  Phe Leu Lys Tyr Tyr  Arg Leu Glu His Leu  Glu Ala Val 
    1190                 1195                 1200             


Phe Glu  Glu Leu Ile Arg Lys  Gln Ala Ala Asp Tyr  Gln Ile Phe 
    1205                 1210                 1215             


Glu Lys  Leu Ile Lys Lys Ile  Glu Val Asn Lys Val  Tyr Phe Tyr 
    1220                 1225                 1230             


Ser Cys  Thr Tyr Asn Glu Lys  Val Lys Val Ile Glu  Glu Leu Leu 
    1235                 1240                 1245             


Lys Ile  Thr Gln Ala Asn Ala  Thr Asn Gly Asp Leu  Lys Leu Leu 
    1250                 1255                 1260             


Lys Met  Ser Asn Arg Glu Gly  Arg Leu Gly Ser Val  Ser Val Ala 
    1265                 1270                 1275             


Leu Asp  Phe Lys Ile Ile Asn  Gln Ser Val Thr Gly  Leu Tyr Gln 
    1280                 1285                 1290             


Ser Ile  Glu Asp Tyr Asn Asn  
    1295                 1300 


<210>  809
<211>  1309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  809

Met Gly Ser Val Gly Phe Ala Gly Ile Asp Asn Gln Tyr Asn Leu Val 
1               5                   10                  15      


Arg Thr Lys Gly Lys Asn Val Ile Gly Val Arg Leu Phe Asp Glu Ala 
            20                  25                  30          


Asp Ser Ala Ala Glu Arg Arg Gly His Arg Thr Asn Arg Arg Arg Leu 
        35                  40                  45              


Gln Arg Arg Arg Trp Arg Leu Arg Leu Leu Asp Asp Ile Phe Ala Lys 
    50                  55                  60                  


Pro Leu Gln Ala Val Asp Pro Asn Phe Leu Ala Arg Leu Lys Tyr Ser 
65                  70                  75                  80  


Tyr Val Asn Lys Lys Asp Gln Gly Gln Gln Asp His Tyr Tyr Gly Gly 
                85                  90                  95      


Tyr Val Phe Gly Ser Thr Ala Ala Asp Gln Ala Tyr His Gln Ala Tyr 
            100                 105                 110         


Pro Thr Ile Tyr His Leu Arg Lys Arg Leu Met Glu Asp Asp Gln Lys 
        115                 120                 125             


His Asp Leu Arg Glu Val Tyr Leu Ala Ile His His Ile Val Lys Tyr 
    130                 135                 140                 


Arg Gly Asn Phe Leu Asn Pro Gln Ser Ser Leu Asp Ile Asp Gln Gln 
145                 150                 155                 160 


Phe Asp Val Thr Asp Phe Ala Gln Ala Leu Ala Arg Phe Ala Asp His 
                165                 170                 175     


Gln Ala Leu Ser Trp Ala Leu Glu Ala Pro Ile Arg Phe Leu Glu Ala 
            180                 185                 190         


Glu Leu Ala Thr Gly Leu Ser Asn Ser Ala Arg Val Asp Ala Ala Ile 
        195                 200                 205             


Glu Ala Phe Ser Phe Asp Thr Lys Val Asp Arg Ala Ala Ile Lys Glu 
    210                 215                 220                 


Met Leu Lys Gly Leu Ser Gly Asn Gln Ile Asp Phe Thr Lys Leu Phe 
225                 230                 235                 240 


Val Asn Val Asp Ser Ala Asp Trp Asp Gln Glu Glu Arg Lys Gln Trp 
                245                 250                 255     


Lys Met Lys Leu Ser Glu Glu Asp Phe Asp Glu Gln Ala Leu Pro Ile 
            260                 265                 270         


Leu Glu Arg Leu Ser Gln Asp Glu Thr Glu Phe Phe Leu Ala Ile Lys 
        275                 280                 285             


Arg Ala Tyr Asp Gly Ile Ala Leu Met Arg Phe Leu Gly Asp Glu Gln 
    290                 295                 300                 


Ser Leu Ser Ser Ala Met Ile Lys Ala Tyr Glu Asp His Arg Arg Asp 
305                 310                 315                 320 


Leu Thr Phe Leu Lys Thr Gln Val Arg Thr Pro Gln Asn Arg Gln Ala 
                325                 330                 335     


Leu Ser Glu Gly Tyr Thr Asn Tyr Leu Ser Val Asp Asp Lys Lys His 
            340                 345                 350         


Lys Arg Gly Ala Lys Glu Leu Ala Gln Leu Ile Glu Ala Ser Asp Ala 
        355                 360                 365             


Ser Glu Gln Asp Lys Ala Thr Met Leu Asp Arg Ile Ala Asn Asp Gln 
    370                 375                 380                 


Phe Ala Pro Lys Gln Arg Thr Lys Ala Asn Gly Leu Ile Pro Tyr Gln 
385                 390                 395                 400 


Leu His Leu Ala Glu Leu Lys Lys Ile Leu Ala Lys Gln Gly Gln Tyr 
                405                 410                 415     


Tyr Pro Phe Leu Leu Asp Thr Phe Ala Lys Gln Gly Gln Ser Val Asn 
            420                 425                 430         


Lys Ile Glu Glu Leu Val Gln Phe Arg Val Pro Tyr Tyr Val Gly Pro 
        435                 440                 445             


Met Val Pro Lys Ser Glu Thr Ala Gly Asn Ala Glu Asn His Trp Val 
    450                 455                 460                 


Glu Lys Asn Asp Gly Gln Thr Lys Val Ser Val Thr Pro Trp Asn Phe 
465                 470                 475                 480 


Asp Gln Val Phe Asn Arg Asp Arg Ala Ala Lys Ser Phe Ile Asp Arg 
                485                 490                 495     


Leu Thr Gly Thr Asp Thr Tyr Leu Ile Gly Glu Pro Thr Leu Pro Arg 
            500                 505                 510         


His Ser Leu Thr Tyr Glu Thr Phe Thr Val Leu Asn Glu Leu Asn Asn 
        515                 520                 525             


Ile Arg Ile Asp Gly Lys Arg Leu Pro Val Glu Thr Lys Gln Ala Ile 
    530                 535                 540                 


Val Glu Asp Leu Phe Lys Lys Tyr Arg Leu Val Thr Lys Lys Arg Leu 
545                 550                 555                 560 


Gln Asp Tyr Phe Ala Ser Phe Gly Lys Arg Glu Val Glu Leu Thr Gly 
                565                 570                 575     


Leu Ala Asp Glu Ser Arg Phe Thr Ser Ser Leu Thr Ser Tyr His Asp 
            580                 585                 590         


Leu Gln Gly Leu Leu Gly Thr Asp Phe Ile Thr Asn Pro Gln Asn His 
        595                 600                 605             


Ser Leu Leu Glu Lys Ile Val Glu Ile Gln Thr Val Phe Glu Asp Ser 
    610                 615                 620                 


Asp Ile Ala Glu Arg Glu Leu Gly Lys Leu Gly Leu Glu Gln Lys Leu 
625                 630                 635                 640 


Ile Pro Arg Leu Ala Lys Lys His Tyr Thr Gly Trp Gly Asn Leu Ser 
                645                 650                 655     


Arg Lys Leu Leu Asp Thr Ser Phe Ile His Asp Pro Glu Arg Pro Glu 
            660                 665                 670         


Glu Pro Val Ser Ile Met Asp Leu Leu Tyr Thr Thr Asn Lys Asn Phe 
        675                 680                 685             


Met Glu Ile Leu His Asp Ser Glu Tyr Gly Val Glu Glu Trp Leu Lys 
    690                 695                 700                 


Ser Gln Asn Met Ile Asp Asp Gln Lys Asp Ile Gln Met Arg Ile Asp 
705                 710                 715                 720 


Glu Leu Thr Thr Ser Pro Ala Asn Lys Arg Gly Ile Lys Gln Ala Phe 
                725                 730                 735     


Asn Val Leu Asp Asp Ile Thr Gln Ala Met Gly Glu Glu Pro Ala Tyr 
            740                 745                 750         


Val Tyr Leu Glu Phe Ala Arg Glu Lys Gln Ala Ser Arg Arg Thr Val 
        755                 760                 765             


Ser Arg Lys Lys Arg Leu Glu Thr Leu Tyr Lys Asn Ala Ala Leu Lys 
    770                 775                 780                 


Thr Glu Phe Lys Ala Ile Lys Glu Ala Leu Ala Glu Glu Ser Asp Asp 
785                 790                 795                 800 


Arg Met Gln Asp Asp Arg Leu Tyr Leu Tyr Tyr Ala Gln Leu Gly Arg 
                805                 810                 815     


Asp Met Tyr Thr Gly Gln Ser Ile Ser Ile Asp Gln Leu Ser Ser His 
            820                 825                 830         


Tyr Asp Ile Asp His Ile Val Pro Arg Ala Phe Ile Lys Asp Asp Ser 
        835                 840                 845             


Leu Glu Asn Lys Val Leu Val Asn Arg Thr Asp Asn Ala Arg Lys Thr 
    850                 855                 860                 


Asp Ser Ala Thr Phe Thr Ala Asp Val Lys Ala Lys Ala Phe Pro Leu 
865                 870                 875                 880 


Trp Gln Gln Leu Lys Lys Leu Gly Leu Ile Ser Ala Lys Lys Phe Arg 
                885                 890                 895     


Leu Leu Thr Arg Thr Gly Asp Phe Thr Glu Met Glu Arg Glu Arg Phe 
            900                 905                 910         


Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys Asn Val Ala 
        915                 920                 925             


Ala Leu Ile Glu Gly His Phe Ser Gln Thr Gln Ala Val Ala Ile Arg 
    930                 935                 940                 


Ala Glu Val Thr Gly Glu Leu Arg Gln Leu Thr Gln Ile Lys Lys Asp 
945                 950                 955                 960 


Arg Asp Ile Asn Asp Tyr His His Ala Gln Asp Ala Leu Leu Val Ala 
                965                 970                 975     


Thr Ala Gly Thr Tyr Leu His Arg His Phe Pro Lys Arg Asp Ala Arg 
            980                 985                 990         


Phe Ile Tyr Asn Glu Phe Asp Tyr  Tyr Thr Gln His Trp  Leu Lys Asn 
        995                 1000                 1005             


Gln Gly  Glu Asn Arg Arg Arg  His Pro Tyr Ser Phe  Val Val Gly 
    1010                 1015                 1020             


Thr Met  Ser Lys Gly Asn Glu  Asp Trp Thr Pro Asp  Asn Leu Asn 
    1025                 1030                 1035             


Tyr Leu  Arg Lys Val Met Gln  Tyr Lys Thr Met Leu  Met Thr Arg 
    1040                 1045                 1050             


Lys Pro  Val Gly Pro Glu Gly  Ala Leu Tyr Lys Glu  Thr Leu Ile 
    1055                 1060                 1065             


Ala Ala  Asp Pro Lys Lys Arg  Leu Val Gly Ala Ser  Lys Glu Arg 
    1070                 1075                 1080             


Gln Asp  Pro Thr Ile Tyr Gly  Gly Tyr Thr Lys Glu  Ser Ser Ala 
    1085                 1090                 1095             


Tyr Met  Ser Leu Val Arg Ala  Gly Gly Lys Asn Gln  Leu Val Lys 
    1100                 1105                 1110             


Ile Pro  Val Arg Ile Ala Asn  Glu Ile His Ser Gly  Gln Arg Lys 
    1115                 1120                 1125             


Leu Asp  Asp Tyr Val Gln Ala  Lys Val Lys Lys Phe  Glu Arg Ile 
    1130                 1135                 1140             


Leu Leu  Pro Lys Ile Ser Leu  Gly Gln Leu Val Glu  Asp Glu Gly 
    1145                 1150                 1155             


Gln Arg  Phe Tyr Leu Ala Thr  Asn Glu Met Lys His  Asn Ala Lys 
    1160                 1165                 1170             


Gln Leu  Trp Leu Asp Gln Lys  Val Val Thr Thr Tyr  Lys Arg Leu 
    1175                 1180                 1185             


Thr Ala  Glu Ser Pro Val Glu  Asp Phe Leu Thr Val  Phe Asp Ala 
    1190                 1195                 1200             


Leu Thr  Ser Ser Ala Thr Ile  His His Phe Lys Phe  Tyr Gln Arg 
    1205                 1210                 1215             


Asp Leu  Glu Leu Leu Arg Asp  Asn Arg Ala Gly Phe  Gln Asp Leu 
    1220                 1225                 1230             


Ala Lys  Ala Thr Gln Leu Lys  Val Leu Lys Asp Val  Leu Tyr Glu 
    1235                 1240                 1245             


Leu His  Asp Asn Ala Gly Trp  Arg Asp Pro Ile Lys  Gln Tyr Phe 
    1250                 1255                 1260             


Lys Glu  Ile Gly Leu Lys Val  Arg Met Trp Thr Lys  Leu Gln Lys 
    1265                 1270                 1275             


Glu Gly  Gly Ile Lys Leu Thr  Asp Gln Ala Glu Leu  Ile Tyr Gln 
    1280                 1285                 1290             


Ser Pro  Ser Gly Leu Phe Glu  Lys Arg Arg Arg Val  Gln Asp Leu 
    1295                 1300                 1305             


Leu 
    


<210>  810
<211>  1312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  810

Met Gln Asn Gly Phe Leu Gly Ile Thr Val Ser Ser Glu Gln Val Gly 
1               5                   10                  15      


Trp Ala Val Thr Asn Pro Lys Tyr Glu Leu Glu Arg Ala Ser Arg Lys 
            20                  25                  30          


Asp Leu Trp Gly Val Arg Leu Phe Asp Lys Ala Glu Thr Ala Glu Asp 
        35                  40                  45              


Arg Arg Met Phe Arg Thr Asn Arg Arg Leu Asn Gln Arg Lys Lys Asn 
    50                  55                  60                  


Arg Ile His Tyr Leu Arg Asp Ile Phe His Glu Glu Val Asn Gln Lys 
65                  70                  75                  80  


Asp Pro Asn Phe Phe Gln Gln Leu Asp Glu Ser Asn Phe Cys Glu Asp 
                85                  90                  95      


Asp Arg Thr Val Glu Phe Asn Phe Asp Thr Asn Leu Tyr Lys Asn Gln 
            100                 105                 110         


Phe Pro Thr Val Tyr His Leu Arg Lys Tyr Leu Met Glu Thr Lys Asp 
        115                 120                 125             


Lys Pro Asp Ile Arg Leu Val Tyr Leu Ala Phe Ser Lys Phe Met Lys 
    130                 135                 140                 


Asn Arg Gly His Phe Leu Tyr Lys Gly Asn Leu Gly Glu Val Met Asp 
145                 150                 155                 160 


Phe Glu Asn Ser Met Lys Gly Phe Cys Glu Ser Leu Glu Lys Phe Asn 
                165                 170                 175     


Ile Asp Phe Pro Thr Leu Ser Asp Glu Gln Val Lys Glu Val Arg Asp 
            180                 185                 190         


Ile Leu Cys Asp His Lys Ile Ala Lys Thr Val Lys Lys Lys Asn Ile 
        195                 200                 205             


Ile Thr Ile Thr Lys Val Lys Ser Lys Thr Ala Lys Ala Trp Ile Gly 
    210                 215                 220                 


Leu Phe Cys Gly Cys Ser Val Pro Val Lys Val Leu Phe Gln Asp Ile 
225                 230                 235                 240 


Asp Glu Glu Ile Val Thr Asp Pro Glu Lys Ile Ser Phe Glu Asp Ala 
                245                 250                 255     


Ser Tyr Asp Asp Tyr Ile Ala Asn Ile Glu Lys Gly Val Gly Ile Tyr 
            260                 265                 270         


Tyr Glu Ala Ile Val Ser Ala Lys Met Leu Phe Asp Trp Ser Ile Met 
        275                 280                 285             


Asn Glu Ile Leu Gly Asp His Gln Leu Leu Ser Asp Ala Met Ile Ala 
    290                 295                 300                 


Glu Tyr Asn Lys His His Asp Asp Leu Lys Arg Leu Gln Lys Ile Ile 
305                 310                 315                 320 


Lys Gly Thr Gly Ser Arg Glu Leu Tyr Gln Asp Ile Phe Ile Asn Asp 
                325                 330                 335     


Val Ser Gly Asn Tyr Val Cys Tyr Val Gly His Ala Lys Thr Met Ser 
            340                 345                 350         


Ser Ala Asp Gln Lys Gln Phe Tyr Thr Phe Leu Lys Asn Arg Leu Lys 
        355                 360                 365             


Asn Val Asn Gly Ile Ser Ser Glu Asp Ala Glu Trp Ile Asp Thr Glu 
    370                 375                 380                 


Ile Lys Asn Gly Thr Leu Leu Pro Lys Gln Thr Lys Arg Asp Asn Ser 
385                 390                 395                 400 


Val Ile Pro His Gln Leu Gln Leu Arg Glu Phe Glu Leu Ile Leu Asp 
                405                 410                 415     


Asn Met Gln Glu Met Tyr Pro Phe Leu Lys Glu Asn Arg Glu Lys Leu 
            420                 425                 430         


Leu Lys Ile Phe Asn Phe Val Ile Pro Tyr Tyr Val Gly Pro Leu Lys 
        435                 440                 445             


Gly Val Val Arg Lys Gly Glu Ser Thr Asn Trp Met Val Pro Lys Lys 
    450                 455                 460                 


Asp Gly Val Ile His Pro Trp Asn Phe Asp Glu Met Val Asp Lys Glu 
465                 470                 475                 480 


Ala Ser Ala Glu Cys Phe Ile Ser Arg Met Thr Gly Asn Cys Ser Tyr 
                485                 490                 495     


Leu Phe Asn Glu Lys Val Leu Pro Lys Asn Ser Leu Leu Tyr Glu Thr 
            500                 505                 510         


Phe Glu Val Leu Asn Glu Leu Asn Pro Leu Lys Ile Asn Gly Glu Pro 
        515                 520                 525             


Ile Ser Val Glu Leu Lys Gln Arg Ile Tyr Glu Gln Leu Phe Leu Thr 
    530                 535                 540                 


Gly Lys Lys Val Thr Lys Lys Ser Leu Thr Lys Tyr Leu Ile Lys Asn 
545                 550                 555                 560 


Gly Tyr Asp Glu Asp Ile Glu Leu Ser Gly Ile Asp Asn Glu Phe His 
                565                 570                 575     


Ser Asn Leu Lys Ser His Ile Asp Phe Glu Asp Tyr Asp Asn Leu Ser 
            580                 585                 590         


Asp Glu Glu Val Glu Arg Ile Ile Leu Arg Ile Thr Val Phe Glu Asp 
        595                 600                 605             


Lys Gln Leu Leu Lys Asp Tyr Leu Asn Arg Glu Phe Val Lys Leu Ser 
    610                 615                 620                 


Glu Asp Glu Arg Lys Gln Ile Cys Ser Leu Ser Tyr Lys Gly Trp Gly 
625                 630                 635                 640 


Asn Leu Ser Glu Met Leu Leu Asn Gly Ile Thr Val Thr Asp Ser Asn 
                645                 650                 655     


Gly Val Glu Val Ser Val Met Asp Met Leu Trp Asn Thr Asn Leu Asn 
            660                 665                 670         


Leu Met Gln Ile Leu Ser Lys Lys Tyr Gly Tyr Lys Ala Glu Ile Glu 
        675                 680                 685             


Arg Tyr Asn Lys Glu His Glu Lys Asn Ile Tyr Asn Arg Glu Asp Leu 
    690                 695                 700                 


Met Asp Tyr Leu Asn Ile Pro Pro Ala Gln Arg Arg Lys Val Asn Gln 
705                 710                 715                 720 


Leu Ile Thr Ile Val Lys Ser Leu Lys Lys Arg Cys Gly Val Pro Asn 
                725                 730                 735     


Lys Ile Phe Phe Lys Val Ser Arg Glu His Gln Asp Asp Pro Lys Arg 
            740                 745                 750         


Thr Ser Ser Arg Lys Glu Gln Leu Lys Tyr Leu Tyr Lys Ser Leu Lys 
        755                 760                 765             


Ser Glu Asp Glu Lys His Leu Met Lys Glu Leu Asp Glu Leu Asn Gly 
    770                 775                 780                 


His Glu Leu Ser Asn Asp Lys Val Tyr Leu Tyr Phe Leu Gln Lys Gly 
785                 790                 795                 800 


Arg Cys Ile Tyr Ser Gly Lys Lys Leu Asn Leu Ser Arg Leu Arg Lys 
                805                 810                 815     


Ser Asn Tyr Gln Asn Asp Ile Asp Tyr Ile Tyr Pro Leu Ser Ala Val 
            820                 825                 830         


Asn Asp Arg Ser Met Asn Asn Lys Val Leu Thr Gly Ile Gln Glu Asn 
        835                 840                 845             


Arg Ala Asp Lys Tyr Thr Tyr Phe Pro Val Asp Pro Glu Ile Gln Lys 
    850                 855                 860                 


Lys Met Lys Gly Phe Trp Met Glu Leu Val Leu Gln Gly Phe Met Ser 
865                 870                 875                 880 


Lys Glu Lys Tyr Phe Arg Leu Ser Arg Glu Asn Asp Phe Ser Glu Asn 
                885                 890                 895     


Glu Leu Val Ser Phe Ile Glu Arg Glu Ile Ser Asp Asn Gln Gln Ser 
            900                 905                 910         


Gly Arg Met Ile Ala Ser Val Leu Gln Tyr Tyr Phe Pro Glu Ser Lys 
        915                 920                 925             


Ile Val Phe Val Lys Glu Lys Leu Ile Ser Ser Phe Lys Arg Asp Phe 
    930                 935                 940                 


His Leu Ile Ser Ser Tyr Gly His Asn His Leu Gln Ala Ala Lys Asp 
945                 950                 955                 960 


Ala Tyr Ile Thr Ile Val Val Gly Asn Val Tyr His Thr Lys Phe Thr 
                965                 970                 975     


Met Asp Pro Ala Ile Tyr Phe Lys Asn His Lys Arg Lys Asp Tyr Asp 
            980                 985                 990         


Leu Asn Arg Leu Phe Leu Glu Asn  Ile Ser Arg Asp Gly  Gln Ile Ala 
        995                 1000                 1005             


Trp Glu  Ser Gly Pro Tyr Gly  Ser Ile Gln Thr Val  Arg Lys Glu 
    1010                 1015                 1020             


Tyr Ala  Gln Asn His Ile Ala  Val Thr Lys Arg Val  Val Glu Val 
    1025                 1030                 1035             


Lys Gly  Gly Leu Phe Lys Gln  Met Pro Leu Lys Lys  Gly His Gly 
    1040                 1045                 1050             


Glu Tyr  Pro Leu Lys Thr Asn  Asp Pro Arg Phe Gly  Asn Ile Ala 
    1055                 1060                 1065             


Gln Tyr  Gly Gly Tyr Thr Asn  Val Thr Gly Ser Tyr  Phe Val Leu 
    1070                 1075                 1080             


Val Glu  Ser Met Glu Lys Gly  Lys Lys Arg Ile Ser  Leu Glu Tyr 
    1085                 1090                 1095             


Val Pro  Val Tyr Leu His Glu  Arg Leu Glu Asp Asp  Pro Gly His 
    1100                 1105                 1110             


Lys Leu  Leu Lys Glu Tyr Leu  Val Asp His Arg Lys  Leu Asn His 
    1115                 1120                 1125             


Pro Lys  Ile Leu Leu Ala Lys  Val Arg Lys Asn Ser  Leu Leu Lys 
    1130                 1135                 1140             


Ile Asp  Gly Phe Tyr Tyr Arg  Leu Asn Gly Arg Ser  Gly Asn Ala 
    1145                 1150                 1155             


Leu Ile  Leu Thr Asn Ala Val  Glu Leu Ile Met Asp  Asp Trp Gln 
    1160                 1165                 1170             


Thr Lys  Thr Ala Asn Lys Ile  Ser Gly Tyr Met Lys  Arg Arg Ala 
    1175                 1180                 1185             


Ile Asp  Lys Lys Ala Arg Val  Tyr Gln Asn Glu Phe  His Ile Gln 
    1190                 1195                 1200             


Glu Leu  Glu Gln Leu Tyr Asp  Phe Tyr Leu Asp Lys  Leu Glu Asn 
    1205                 1210                 1215             


Gly Val  Tyr Lys Asn Arg Lys  Asn Asn Gln Ala Glu  Leu Ile His 
    1220                 1225                 1230             


Asn Glu  Lys Glu Gln Phe Met  Glu Leu Lys Thr Glu  Asp Gln Cys 
    1235                 1240                 1245             


Val Leu  Leu Thr Glu Ile Lys  Lys Leu Phe Val Cys  Ser Pro Met 
    1250                 1255                 1260             


Gln Ala  Asp Leu Thr Leu Ile  Gly Gly Ser Lys His  Thr Gly Met 
    1265                 1270                 1275             


Ile Ala  Met Ser Ser Asn Val  Thr Lys Ala Asp Phe  Ser Ile Ile 
    1280                 1285                 1290             


Ala Glu  Asp Pro Leu Gly Leu  Arg Asn Lys Val Ile  Tyr Ser His 
    1295                 1300                 1305             


Met Gly  Glu Lys 
    1310         


<210>  811
<211>  1312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  811

Met Gln Asn Gly Phe Leu Gly Ile Thr Val Ser Ser Glu Gln Val Gly 
1               5                   10                  15      


Trp Ala Val Thr Asn Pro Lys Tyr Glu Leu Glu Arg Ala Ser Arg Lys 
            20                  25                  30          


Asp Leu Trp Gly Val Arg Leu Phe Asp Lys Ala Glu Thr Ala Glu Asp 
        35                  40                  45              


Arg Arg Met Phe Arg Thr Asn Arg Arg Leu Asn Gln Arg Lys Lys Asn 
    50                  55                  60                  


Arg Ile His Tyr Leu Arg Asp Ile Phe His Glu Glu Val Asn Gln Lys 
65                  70                  75                  80  


Asp Pro Asn Phe Phe Gln Gln Leu Asp Glu Ser Asn Phe Cys Glu Asp 
                85                  90                  95      


Asp Arg Thr Val Glu Phe Asn Phe Asp Thr Asn Leu Tyr Lys Asn Gln 
            100                 105                 110         


Phe Pro Thr Val Tyr His Leu Arg Lys Tyr Leu Met Glu Thr Lys Asp 
        115                 120                 125             


Lys Pro Asp Ile Arg Leu Val Tyr Leu Ala Phe Ser Lys Phe Met Lys 
    130                 135                 140                 


Asn Arg Gly His Phe Leu Tyr Lys Gly Asn Leu Gly Glu Val Met Asp 
145                 150                 155                 160 


Phe Glu Asn Ser Met Lys Gly Phe Cys Glu Ser Leu Glu Lys Phe Asn 
                165                 170                 175     


Ile Asp Phe Pro Thr Leu Ser Asp Glu Gln Val Lys Glu Val Arg Asp 
            180                 185                 190         


Ile Leu Cys Asp His Lys Ile Ala Lys Thr Val Lys Lys Lys Asn Ile 
        195                 200                 205             


Ile Thr Ile Thr Lys Val Lys Ser Lys Thr Ala Lys Ala Trp Ile Gly 
    210                 215                 220                 


Leu Phe Cys Gly Cys Ser Val Pro Val Lys Val Leu Phe Gln Asp Ile 
225                 230                 235                 240 


Asp Glu Glu Ile Val Thr Asp Pro Glu Lys Ile Ser Phe Glu Asp Ala 
                245                 250                 255     


Ser Tyr Asp Asp Tyr Ile Ala Asn Ile Glu Lys Gly Val Gly Ile Tyr 
            260                 265                 270         


Tyr Glu Ala Ile Val Ser Ala Lys Met Leu Phe Asp Trp Ser Ile Leu 
        275                 280                 285             


Asn Glu Ile Leu Gly Asp His Gln Leu Leu Ser Asp Ala Met Ile Ala 
    290                 295                 300                 


Glu Tyr Asn Lys His His Asp Asp Leu Lys Arg Leu Gln Lys Ile Ile 
305                 310                 315                 320 


Lys Gly Thr Gly Ser Arg Glu Leu Tyr Gln Asp Ile Phe Ile Asn Asp 
                325                 330                 335     


Val Ser Gly Asn Tyr Val Cys Tyr Val Gly His Ala Lys Thr Met Ser 
            340                 345                 350         


Ser Ala Asp Gln Lys Gln Phe Tyr Thr Phe Leu Lys Asn Arg Leu Lys 
        355                 360                 365             


Asn Val Asn Gly Ile Ser Ser Glu Asp Ala Glu Trp Ile Asp Thr Glu 
    370                 375                 380                 


Ile Lys Asn Gly Thr Leu Leu Pro Lys Gln Thr Lys Arg Asp Asn Ser 
385                 390                 395                 400 


Val Ile Pro His Gln Leu Gln Leu Arg Glu Phe Glu Leu Ile Leu Asp 
                405                 410                 415     


Asn Met Gln Glu Met Tyr Pro Phe Leu Lys Glu Asn Arg Glu Lys Leu 
            420                 425                 430         


Leu Lys Ile Phe Asn Phe Val Ile Pro Tyr Tyr Val Gly Pro Leu Lys 
        435                 440                 445             


Gly Val Val Arg Lys Gly Glu Ser Thr Asn Trp Met Val Pro Lys Lys 
    450                 455                 460                 


Asp Gly Val Ile His Pro Trp Asn Phe Asp Glu Met Val Asp Lys Glu 
465                 470                 475                 480 


Ala Ser Ala Glu Cys Phe Ile Ser Arg Met Thr Gly Asn Cys Ser Tyr 
                485                 490                 495     


Leu Phe Asn Glu Lys Val Leu Pro Lys Asn Ser Leu Leu Tyr Glu Thr 
            500                 505                 510         


Phe Glu Val Leu Asn Glu Leu Asn Pro Leu Lys Ile Asn Gly Glu Ser 
        515                 520                 525             


Ile Ser Val Glu Leu Lys Gln Arg Ile Tyr Glu Gln Leu Phe Leu Thr 
    530                 535                 540                 


Gly Lys Lys Val Thr Lys Lys Ser Leu Thr Lys Tyr Leu Ile Lys Asn 
545                 550                 555                 560 


Gly Tyr Asp Lys Asp Ile Glu Leu Ser Gly Ile Asp Asn Glu Phe His 
                565                 570                 575     


Ser Asn Leu Lys Ser His Ile Asp Phe Glu Asp Tyr Asp Asn Leu Ser 
            580                 585                 590         


Asp Glu Glu Val Glu Gln Ile Ile Leu Arg Ile Thr Val Phe Glu Asp 
        595                 600                 605             


Lys Gln Leu Leu Lys Asp Tyr Leu Asn Arg Glu Phe Val Lys Leu Ser 
    610                 615                 620                 


Glu Asp Glu Arg Lys Gln Ile Cys Ser Leu Ser Tyr Lys Gly Trp Gly 
625                 630                 635                 640 


Asn Leu Ser Glu Met Leu Leu Asn Gly Ile Thr Val Thr Asp Ser Asn 
                645                 650                 655     


Gly Val Glu Val Ser Val Met Asp Met Leu Trp Asn Thr Asn Leu Asn 
            660                 665                 670         


Leu Met Gln Ile Leu Ser Lys Lys Tyr Gly Tyr Lys Ala Glu Ile Glu 
        675                 680                 685             


His Tyr Asn Lys Glu His Glu Lys Thr Ile Tyr Asn Arg Glu Asp Leu 
    690                 695                 700                 


Met Asp Tyr Leu Asn Ile Pro Pro Ala Gln Arg Arg Lys Val Asn Gln 
705                 710                 715                 720 


Leu Ile Thr Ile Val Lys Ser Leu Lys Lys Thr Tyr Gly Val Pro Asn 
                725                 730                 735     


Lys Ile Phe Phe Lys Val Ser Arg Glu His Gln Asp Asp Pro Lys Arg 
            740                 745                 750         


Thr Ser Ser Arg Lys Glu Gln Leu Lys Tyr Leu Tyr Lys Ser Leu Lys 
        755                 760                 765             


Ser Glu Asp Glu Lys His Leu Met Lys Glu Leu Asp Glu Leu Asn Gly 
    770                 775                 780                 


His Glu Leu Ser Asn Asp Lys Val Tyr Leu Tyr Phe Leu Gln Lys Gly 
785                 790                 795                 800 


Arg Cys Ile Tyr Ser Gly Lys Lys Leu Asn Leu Ser Arg Leu Arg Lys 
                805                 810                 815     


Ser Asn Tyr Gln Asn Asp Ile Asp Tyr Ile Tyr Pro Leu Ser Ala Val 
            820                 825                 830         


Asn Asp Arg Ser Met Asn Asn Lys Val Leu Thr Gly Ile Gln Glu Asn 
        835                 840                 845             


Arg Ala Asp Lys Tyr Thr Tyr Phe Pro Val Asp Pro Glu Ile Gln Lys 
    850                 855                 860                 


Lys Met Lys Gly Phe Trp Met Glu Leu Val Leu Gln Gly Phe Met Ser 
865                 870                 875                 880 


Lys Glu Lys Tyr Phe Arg Leu Ser Arg Glu Asn Asp Phe Ser Glu Asn 
                885                 890                 895     


Glu Leu Val Ser Phe Ile Glu Arg Glu Ile Ser Asp Asn Gln Gln Ser 
            900                 905                 910         


Gly Arg Met Ile Ala Ser Val Leu Gln Tyr Tyr Phe Pro Glu Ser Lys 
        915                 920                 925             


Ile Val Phe Val Lys Glu Lys Leu Ile Ser Ser Phe Lys Arg Asp Phe 
    930                 935                 940                 


His Leu Ile Ser Ser Tyr Gly His Asn His Leu Gln Ala Ala Lys Asp 
945                 950                 955                 960 


Ala Tyr Ile Thr Ile Val Val Gly Asn Val Tyr His Ala Lys Phe Thr 
                965                 970                 975     


Met Asp Pro Ala Ile Tyr Phe Lys Asn His Lys Arg Lys Asp Tyr Asp 
            980                 985                 990         


Leu Asn Arg Leu Phe Leu Glu Asn  Ile Ser Arg Asp Gly  Gln Ile Ala 
        995                 1000                 1005             


Trp Glu  Ser Gly Pro Tyr Gly  Ser Ile Gln Thr Val  Arg Lys Glu 
    1010                 1015                 1020             


Tyr Ala  Gln Asn His Ile Ala  Val Thr Lys Arg Val  Val Glu Val 
    1025                 1030                 1035             


Lys Gly  Gly Leu Phe Lys Gln  Met Pro Leu Lys Lys  Gly His Gly 
    1040                 1045                 1050             


Glu Tyr  Pro Leu Lys Thr Asn  Asp Pro Arg Phe Gly  Asn Ile Ala 
    1055                 1060                 1065             


Gln Tyr  Gly Gly Tyr Thr Asn  Val Thr Gly Ser Tyr  Phe Val Leu 
    1070                 1075                 1080             


Val Glu  Ser Met Glu Lys Gly  Lys Lys Arg Ile Ser  Leu Glu Tyr 
    1085                 1090                 1095             


Val Pro  Val Tyr Leu His Glu  Arg Leu Glu Asp Asp  Pro Gly His 
    1100                 1105                 1110             


Lys Leu  Leu Lys Glu Tyr Leu  Val Asp His Arg Lys  Leu Asn His 
    1115                 1120                 1125             


Pro Lys  Ile Leu Leu Ala Lys  Val Arg Lys Asn Ser  Leu Leu Lys 
    1130                 1135                 1140             


Ile Asp  Gly Phe Tyr Tyr Arg  Leu Asn Gly Arg Ser  Gly Asn Ala 
    1145                 1150                 1155             


Leu Ile  Leu Thr Asn Ala Val  Glu Leu Ile Met Asp  Asp Trp Gln 
    1160                 1165                 1170             


Thr Lys  Thr Ala Asn Lys Ile  Ser Gly Tyr Met Lys  Arg Arg Ala 
    1175                 1180                 1185             


Ile Asp  Lys Lys Ala Arg Val  Tyr Gln Asn Glu Phe  His Ile Gln 
    1190                 1195                 1200             


Glu Leu  Glu Gln Leu Tyr Asp  Phe Tyr Leu Asp Lys  Leu Glu Asn 
    1205                 1210                 1215             


Gly Val  Tyr Lys Asn Arg Lys  Asn Asn Gln Ala Glu  Leu Ile His 
    1220                 1225                 1230             


Asn Glu  Lys Glu Gln Phe Met  Glu Leu Lys Thr Glu  Asp Gln Cys 
    1235                 1240                 1245             


Val Leu  Leu Thr Glu Ile Lys  Lys Leu Phe Val Cys  Ser Pro Met 
    1250                 1255                 1260             


Gln Ala  Asp Leu Thr Leu Ile  Gly Gly Ser Lys His  Thr Gly Met 
    1265                 1270                 1275             


Ile Ala  Met Ser Ser Asn Val  Thr Lys Ala Asp Phe  Ala Val Ile 
    1280                 1285                 1290             


Ala Glu  Asp Pro Leu Gly Leu  Arg Asn Lys Val Ile  Tyr Ser His 
    1295                 1300                 1305             


Met Gly  Glu Lys 
    1310         


<210>  812
<211>  1313
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  812

Met His Tyr Leu Asp Glu Ile Phe Ala Pro His Leu Gln Glu Val Asp 
1               5                   10                  15      


Glu Asn Phe Leu Arg Arg Leu Lys Gln Ser Asn Ile His Pro Glu Asp 
            20                  25                  30          


Pro Ala Lys Asn Gln Ala Phe Ile Gly Lys Leu Leu Phe Pro Asp Leu 
        35                  40                  45              


Leu Lys Lys Asn Glu Arg Gly Tyr Pro Thr Leu Ile Lys Met Arg Asp 
    50                  55                  60                  


Glu Leu Pro Val Glu Gln Arg Ala His Tyr Pro Val Thr Asn Ile Tyr 
65                  70                  75                  80  


Lys Leu Arg Glu Ala Met Ile Asn Glu Asp Arg Gln Phe Asp Leu Arg 
                85                  90                  95      


Glu Val Tyr Leu Ala Val His His Ile Met Lys Tyr Arg Gly His Phe 
            100                 105                 110         


Leu Asn Asn Ala Ser Val Asp Lys Phe Lys Val Gly Arg Ile Asp Phe 
        115                 120                 125             


Tyr Lys Ser Phe Asn Val Leu Asn Glu Ala Tyr Glu Glu Leu Gln Asn 
    130                 135                 140                 


Gly Glu Gly Ser Phe Thr Ile Glu Pro Ser Lys Val Glu Lys Ile Gly 
145                 150                 155                 160 


Gln Leu Leu Leu Asp Thr Lys Met Arg Lys Leu Asn Arg Gln Lys Ala 
                165                 170                 175     


Val Ala Lys Leu Leu Glu Val Lys Val Ala Asp Lys Glu Glu Thr Lys 
            180                 185                 190         


Arg Asn Lys Gln Ile Ala Thr Ala Met Ser Lys Leu Val Leu Gly Tyr 
        195                 200                 205             


Lys Ala Asp Phe Ala Thr Val Ala Met Ala Asn Gly Asn Glu Trp Lys 
    210                 215                 220                 


Ile Asp Leu Ser Ser Glu Thr Ser Glu Asp Glu Ile Glu Lys Phe Arg 
225                 230                 235                 240 


Glu Glu Leu Ser Asp Ala Gln Asn Asp Ile Leu Thr Glu Ile Thr Ser 
                245                 250                 255     


Leu Phe Ser Gln Ile Met Leu Asn Glu Ile Val Pro Asn Gly Met Ser 
            260                 265                 270         


Ile Ser Glu Ser Met Met Asp Arg Tyr Trp Thr His Glu Arg Gln Leu 
        275                 280                 285             


Ala Glu Val Lys Glu Tyr Leu Ala Thr Gln Pro Ala Ser Ala Arg Lys 
    290                 295                 300                 


Glu Phe Asp Gln Val Tyr Asn Lys Tyr Ile Gly Gln Ala Pro Lys Glu 
305                 310                 315                 320 


Arg Gly Phe Asp Leu Glu Lys Gly Leu Lys Lys Ile Leu Ser Lys Lys 
                325                 330                 335     


Glu Asn Trp Lys Glu Ile Asp Glu Leu Leu Lys Ala Gly Asp Phe Leu 
            340                 345                 350         


Pro Lys Gln Arg Thr Ser Ala Asn Gly Val Ile Pro His Gln Met His 
        355                 360                 365             


Gln Gln Glu Leu Asp Arg Ile Ile Glu Lys Gln Ala Lys Tyr Tyr Pro 
    370                 375                 380                 


Trp Leu Ala Thr Glu Asn Pro Ala Thr Gly Glu Arg Asp Arg His Gln 
385                 390                 395                 400 


Ala Lys Tyr Glu Leu Asp Gln Leu Val Ser Phe Arg Ile Pro Tyr Tyr 
                405                 410                 415     


Val Gly Pro Leu Val Thr Pro Glu Val Gln Lys Ala Thr Ser Gly Ala 
            420                 425                 430         


Lys Phe Ala Trp Ala Lys Arg Lys Glu Asp Gly Glu Ile Thr Pro Trp 
        435                 440                 445             


Asn Leu Trp Asp Lys Ile Asp Arg Ala Glu Ser Ala Glu Ala Phe Ile 
    450                 455                 460                 


Lys Arg Met Thr Val Lys Asp Thr Tyr Leu Leu Asn Glu Asp Val Leu 
465                 470                 475                 480 


Pro Ala Asn Ser Leu Leu Tyr Gln Lys Tyr Asn Val Leu Asn Glu Leu 
                485                 490                 495     


Asn Asn Val Arg Val Asn Gly Arg Arg Leu Ser Val Gly Ile Lys Gln 
            500                 505                 510         


Asp Ile Tyr Thr Glu Leu Phe Lys Lys Lys Lys Thr Val Lys Val Ser 
        515                 520                 525             


Asp Val Ala Ser Leu Val Met Ala Lys Thr Arg Gly Val Asn Lys Pro 
    530                 535                 540                 


Ser Val Glu Gly Leu Ser Asp Pro Lys Lys Phe Asn Ser Asn Leu Ala 
545                 550                 555                 560 


Thr Tyr Leu Asp Leu Lys Ser Ile Val Gly Asp Lys Val Asp Asp Asn 
                565                 570                 575     


Arg Tyr Gln Thr Asp Leu Glu Asn Ile Ile Glu Trp Arg Ser Val Phe 
            580                 585                 590         


Glu Asp Gly Glu Ile Phe Ala Asp Lys Leu Thr Glu Val Glu Trp Leu 
        595                 600                 605             


Thr Asp Gln Gln Arg Ser Ala Leu Val Lys Lys Arg Tyr Lys Gly Trp 
    610                 615                 620                 


Gly Arg Leu Ser Lys Lys Leu Leu Thr Gly Ile Val Asp Glu Asn Gly 
625                 630                 635                 640 


Gln Arg Ile Ile Asp Leu Met Trp Asn Thr Asp Gln Asn Phe Met Gln 
                645                 650                 655     


Ile Val Asn Gln Pro Val Phe Lys Glu Gln Ile Asp Gln Leu Asn Gln 
            660                 665                 670         


Lys Ala Ile Thr Asn Asp Gly Met Thr Leu Arg Glu Trp Val Glu Ser 
        675                 680                 685             


Val Leu Asp Gly Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Trp 
    690                 695                 700                 


Gln Val Val Arg Val Val Glu Asp Ile Val Lys Ala Val Gly Asn Ala 
705                 710                 715                 720 


Pro Lys Ser Ile Ser Ile Glu Phe Ala Arg Asn Glu Gly Asn Lys Gly 
                725                 730                 735     


Glu Ile Thr Arg Ser Arg Arg Thr Gln Leu Gln Lys Leu Phe Glu Asp 
            740                 745                 750         


Gln Ala His Glu Leu Val Lys Asp Thr Ser Leu Thr Glu Glu Leu Glu 
        755                 760                 765             


Lys Ala Pro Asp Leu Ser Asp Arg Tyr Tyr Leu Tyr Phe Thr Gln Gly 
    770                 775                 780                 


Gly Lys Asp Met Tyr Thr Gly Asp Pro Ile Asn Phe Asp Glu Ile Ser 
785                 790                 795                 800 


Thr Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe Val Lys Asp 
                805                 810                 815     


Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Ala Glu Asn Asn Lys 
            820                 825                 830         


Lys Ser Asp Arg Val Pro Ala Lys Leu Tyr Ala Ala Lys Met Lys Pro 
        835                 840                 845             


Tyr Trp Asn Gln Leu Leu Lys Gln Gly Leu Ile Thr Gln Arg Lys Phe 
    850                 855                 860                 


Glu Asn Leu Thr Met Asp Val Asp Gln Asn Ile Lys Tyr Arg Ser Leu 
865                 870                 875                 880 


Gly Phe Val Lys Arg Gln Leu Val Glu Thr Arg Gln Val Ile Lys Leu 
                885                 890                 895     


Thr Ala Asn Ile Leu Gly Ser Met Tyr Gln Glu Ala Gly Thr Asp Ile 
            900                 905                 910         


Ile Glu Thr Arg Ala Gly Leu Thr Lys Gln Leu Arg Glu Glu Phe Asp 
        915                 920                 925             


Leu Pro Lys Val Arg Glu Val Asn Asp Tyr His His Ala Val Asp Ala 
    930                 935                 940                 


Tyr Leu Thr Thr Phe Ala Gly Gln Tyr Leu Asn Arg Arg Tyr Pro Lys 
945                 950                 955                 960 


Leu Arg Ser Phe Phe Val Tyr Gly Glu Tyr Met Lys Phe Lys His Gly 
                965                 970                 975     


Ser Asp Leu Lys Leu Arg Asn Phe Asn Phe Phe His Glu Leu Met Glu 
            980                 985                 990         


Gly Asp Lys Ser Gln Gly Lys Val  Val Asp Gln Gln Thr  Gly Glu Leu 
        995                 1000                 1005             


Ile Thr  Thr Arg Asp Glu Val  Ala Lys Ser Phe Asp  Arg Leu Leu 
    1010                 1015                 1020             


Asn Met  Lys Tyr Met Leu Val  Ser Lys Glu Val His  Asp Arg Ser 
    1025                 1030                 1035             


Asp Gln  Leu Tyr Gly Ala Thr  Ile Val Thr Ala Lys  Glu Ser Gly 
    1040                 1045                 1050             


Lys Leu  Thr Ser Pro Ile Glu  Ile Lys Lys Lys Arg  Pro Val Asp 
    1055                 1060                 1065             


Leu Tyr  Gly Ala Tyr Thr Asn  Gly Thr Ser Ala Phe  Met Thr Ile 
    1070                 1075                 1080             


Ile Lys  Phe Thr Gly Asn Lys  Pro Lys Tyr Lys Val  Ile Gly Val 
    1085                 1090                 1095             


Pro Thr  Thr Ser Ala Val Gly  Leu Lys Arg Val Gly  Lys Pro Gly 
    1100                 1105                 1110             


Ser Glu  Ser Tyr Asn Gln Glu  Leu His Arg Ile Ile  Lys Ser Asn 
    1115                 1120                 1125             


Pro Lys  Val Lys Lys Asp Phe  Glu Ile Val Val Pro  His Val Ser 
    1130                 1135                 1140             


Tyr Ser  Gln Leu Ile Val Asp  Asp Asp Cys Lys Phe  Thr Leu Ala 
    1145                 1150                 1155             


Ser Asp  Thr Tyr Gln His Pro  Ala Thr Gln Leu Val  Leu Ser Lys 
    1160                 1165                 1170             


Glu Ser  Met Glu Ile Ile Ala  Asp Asp Phe Lys Phe  Val Lys Glu 
    1175                 1180                 1185             


Asn Thr  Ala Thr Ala Asp Glu  Gln Leu Val Arg Val  Phe Asp Glu 
    1190                 1195                 1200             


Ile Val  Asn Gln Met Asn His  Tyr Phe Thr Ile Phe  Asp Gln Arg 
    1205                 1210                 1215             


Ser Asn  Arg Gln Lys Val Thr  Lys Ala Arg Asp Lys  Phe Val Ser 
    1220                 1225                 1230             


Leu Pro  Thr Glu Ser Glu Tyr  Glu Gly Ala Lys Lys  Thr Gln Val 
    1235                 1240                 1245             


Gly Lys  Thr Glu Val Ile Thr  Asn Leu Leu Met Gly  Leu His Ala 
    1250                 1255                 1260             


Asn Ser  Ala Gln Gly Asp Leu  Lys Val Leu Gly Leu  Ser Thr Phe 
    1265                 1270                 1275             


Gly Phe  Phe Gln Ser Ser Gly  Gly Leu Asn Leu Ser  Glu Asp Ala 
    1280                 1285                 1290             


Met Ile  Val Tyr Gln Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Ile 
    1295                 1300                 1305             


Cys Leu  Lys Asp Ile 
    1310             


<210>  813
<211>  1313
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  813

Met Ser Phe Lys Glu Asn Ser Lys Ile Tyr Tyr Gly Leu Asp Ile Gly 
1               5                   10                  15      


Thr Asp Ser Val Gly Trp Ser Thr Thr Asn Glu Lys Tyr Glu Leu Leu 
            20                  25                  30          


Arg Tyr Lys Asn Asn Leu Met Trp Gly Val Ser Leu Phe Glu Ala Ala 
        35                  40                  45              


Ser Leu Ala Glu Glu Arg Arg Gly His Arg Thr Gln Arg Arg Arg Tyr 
    50                  55                  60                  


Asp Arg Arg Gln Gln Arg Val Ala Leu Leu Gly Glu Leu Phe Ala Lys 
65                  70                  75                  80  


Glu Ile Leu Lys Thr Asp Pro Asp Phe Phe Leu Arg Leu Lys Glu Ser 
                85                  90                  95      


Ser Leu Tyr Pro Glu Asp Arg Thr Ser Lys Asn Val Asn Thr Tyr Phe 
            100                 105                 110         


Asp Asp Ala Asp Phe Lys Asp Ser Asp Tyr Phe Lys Met Tyr Pro Thr 
        115                 120                 125             


Val His His Leu Ile Lys Glu Leu Ser Glu Ser Asn Lys Pro His Asp 
    130                 135                 140                 


Val Arg Leu Val Tyr Leu Ala Cys Ala Phe Ile Val Ala His Arg Gly 
145                 150                 155                 160 


His Phe Leu Asn Gly Ala Asp Glu Asn Asn Val Gln Ala Val Leu Asp 
                165                 170                 175     


Phe Asp Ser Ser Tyr Cys Glu Phe Thr Asp Trp Phe Lys Ser Asn Asp 
            180                 185                 190         


Ile Glu Asp Asn Pro Phe Ser Glu Ser Val Lys Asn Glu Phe Ser Val 
        195                 200                 205             


Ile Leu Arg Lys Lys Ile Gly Ile Thr Ala Lys Glu Arg Glu Ile Lys 
    210                 215                 220                 


Asn Leu Leu Phe Gly Thr Thr Lys Thr Pro Asp Cys Tyr Lys Asp Glu 
225                 230                 235                 240 


Glu Tyr Pro Ile Asp Ile Asp Val Leu Ile Lys Phe Ile Ser Gly Gly 
                245                 250                 255     


Lys Thr Asn Leu Ala Lys Leu Phe Arg Asn Pro Ser Tyr Asp Glu Leu 
            260                 265                 270         


Asp Ile Gln Thr Val Glu Val Gly Lys Ala Asp Phe Ala Asp Thr Ile 
        275                 280                 285             


Asp Leu Leu Ala Ser Ser Met Glu Asp Thr Asp Val Pro Leu Leu Ser 
    290                 295                 300                 


Ala Val Lys Ala Met Tyr Asp Trp Ser Leu Leu Ile Asp Val Leu Lys 
305                 310                 315                 320 


Gly Gln Lys Thr Ile Ser Asp Ala Lys Val Cys Glu Tyr Glu Gln His 
                325                 330                 335     


Lys Ser Asp Leu Lys Ala Leu Lys Tyr Ile Val Arg Lys Tyr Leu Asp 
            340                 345                 350         


Arg Ala Gln Tyr Asp Glu Ile Phe Arg Thr Ala Gly Glu Lys Pro Asn 
        355                 360                 365             


Tyr Val Ser Tyr Ser Tyr Asn Val Thr Asp Val Lys Leu Lys Gln Leu 
    370                 375                 380                 


Pro Ser Asn Phe Lys Lys Lys Asn Ser Glu Glu Phe Cys Lys Tyr Ile 
385                 390                 395                 400 


Lys Ser Lys Leu Glu Lys Ile Lys Pro Glu Ser Asp Asp Glu Ala Val 
                405                 410                 415     


Tyr Asn Glu Leu Ile Glu Lys Cys Asn Ser Lys Thr Leu Cys Pro Lys 
            420                 425                 430         


Gln Val Thr Asp Glu Asn Arg Val Ile Pro Tyr Gln Leu Tyr Tyr His 
        435                 440                 445             


Glu Leu Ser Met Ile Leu Asp Lys Ala Ser Ala Tyr Leu Asp Phe Leu 
    450                 455                 460                 


Asn Lys Thr Glu Asp Gly Ile Ser Val Lys Gln Lys Ile Leu Thr Leu 
465                 470                 475                 480 


Met Lys Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Val Lys Arg Asn 
                485                 490                 495     


Glu Thr Asp Asn Val Trp Ile Val Arg Lys Ala Glu Gly Arg Ile Tyr 
            500                 505                 510         


Pro Trp Asn Phe Glu Asn Met Val Asp Phe Asp Lys Ser Glu Asp Gly 
        515                 520                 525             


Phe Ile Arg Arg Met Thr Cys Lys Cys Thr Tyr Leu Ala Gly Glu Asp 
    530                 535                 540                 


Val Leu Pro Lys Tyr Ser Leu Leu Tyr Ser Lys Tyr Thr Val Leu Asn 
545                 550                 555                 560 


Glu Ile Asn Asn Ile Lys Val Asn Gly Glu Lys Ile Ser Pro Glu Leu 
                565                 570                 575     


Lys Gln Asp Ile Phe Asn Glu Leu Phe Met Lys Thr Ser Arg Val Thr 
            580                 585                 590         


Val Lys Lys Ile Thr Glu Leu Leu Lys Arg Lys Gly Ala Phe Ser Glu 
        595                 600                 605             


Glu Lys Gly Asp Ser Leu Ser Gly Val Asp Ile Asn Ile Lys Ser Ser 
    610                 615                 620                 


Leu Lys Ser Tyr Leu Asp Phe Arg Arg Leu Leu Glu Asn Gly Ser Leu 
625                 630                 635                 640 


Ser Glu Asn Asp Val Glu Arg Ile Ile Glu Arg Ile Thr Val Thr Thr 
                645                 650                 655     


Asp Lys Pro Arg Leu Val Ser Trp Leu Lys Ala Glu Tyr Ser Asp Leu 
            660                 665                 670         


Pro Ala Glu Asp Ile Arg Tyr Ile Ser Arg Leu Ser Tyr Lys Asp Tyr 
        675                 680                 685             


Gly Arg Leu Ser Ala Lys Leu Leu Thr Gly Cys Tyr Glu Leu Asp Thr 
    690                 695                 700                 


Asn Thr Gly Glu Ile Gly Gly Arg Ser Ile Ile Asp Phe Met Trp Ala 
705                 710                 715                 720 


Glu Asn Ile Asn Leu Met Gln Ile Leu Ser Asp Ser Tyr Gly Tyr Lys 
                725                 730                 735     


Ser Phe Ile Glu Glu Glu Asn Lys Lys Tyr Tyr Met Ile Asn Pro Thr 
            740                 745                 750         


Gly Ser Ile Ala Gln Thr Leu Arg Glu Met Tyr Val Ser Pro Ser Val 
        755                 760                 765             


Ser Arg Ala Ile Ile Arg Thr Met Glu Ile Val Lys Glu Leu Arg Lys 
    770                 775                 780                 


Ile Thr Lys Lys Asp Pro Asp Lys Ile Phe Val Glu Met Ala Arg Gly 
785                 790                 795                 800 


Gly Lys Pro Glu Glu Lys Gly Lys Arg Thr Ser Ser Arg Arg Glu Gln 
                805                 810                 815     


Ile Glu Lys Leu Tyr Asp Ser Ala Lys Ala Phe Val Ser Asp Glu Asp 
            820                 825                 830         


Ile Ser His Met Arg Ser Gln Leu Gly Ser Leu Ser Asp Glu Gln Leu 
        835                 840                 845             


Arg Arg Glu Lys Tyr Tyr Leu Tyr Phe Ile Gln Phe Gly Lys Cys Met 
    850                 855                 860                 


Tyr Ser Gly Glu Ala Ile Asp Phe Ser Arg Leu Gly Asp Asn His Cys 
865                 870                 875                 880 


Tyr Asp Ile Asp His Ile Phe Pro Gln Ser Lys Ile Asn Asp Asp Ser 
                885                 890                 895     


Leu His Asn Lys Val Leu Val Lys Ser Gln Leu Asn Gly Glu Lys Ser 
            900                 905                 910         


Asp Asp Tyr Pro Ile Lys Ala Glu Ile Arg Asn Lys Met His Pro Leu 
        915                 920                 925             


Trp Lys Asn Leu Phe Tyr Arg Asp Pro Lys Asn Pro Ala Asp Lys Val 
    930                 935                 940                 


Lys Tyr Glu Arg Leu Thr Arg Ser Thr Pro Phe Thr Glu Asp Glu Leu 
945                 950                 955                 960 


Ala Gly Phe Ile Glu Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys 
                965                 970                 975     


Ala Val Ala Thr Leu Leu Lys Glu Met Phe Pro Asp Ser Lys Ile Val 
            980                 985                 990         


Tyr Val Lys Ala Gly Gln Val Ser  Glu Phe Arg His Asp  Phe Asp Met 
        995                 1000                 1005             


Leu Lys  Cys Arg Glu Ile Asn  Asp Leu His His Ala  Lys Asp Ala 
    1010                 1015                 1020             


Tyr Leu  Asn Ile Val Ile Gly  Asn Val His Asp Val  Lys Phe Thr 
    1025                 1030                 1035             


Ser Asn  Pro Met Asn Phe Val  Lys Asn Ala Asp Lys  His Tyr Thr 
    1040                 1045                 1050             


Ile Lys  Val Lys Glu Thr Leu  Lys His Lys Val Ser  Arg Asn Gly 
    1055                 1060                 1065             


Glu Thr  Ala Trp Asn Pro Glu  Thr Asp Phe Asp Thr  Val Lys Arg 
    1070                 1075                 1080             


Met Met  Ser Lys Asn Ser Val  Arg Tyr Val Arg Tyr  Cys Tyr Lys 
    1085                 1090                 1095             


Arg Lys  Gly Gly Phe Phe Asn  Gln Asn Pro Asp Arg  Ala Gly Asn 
    1100                 1105                 1110             


Pro Asp  Leu Ala Arg Leu Lys  Lys Asp Leu Asp Pro  Val Lys Tyr 
    1115                 1120                 1125             


Gly Gly  Tyr Asn Ser Lys Ser  Ile Ser Cys Phe Ser  Leu Ile Lys 
    1130                 1135                 1140             


Cys Thr  Gly Val Gly Ala Val  Ile Ile Pro Val Glu  Leu Leu Cys 
    1145                 1150                 1155             


Glu Lys  Arg Tyr Phe Ser Asp  Asp Ser Phe Ala Ser  Glu Tyr Ala 
    1160                 1165                 1170             


Tyr Ser  Val Leu Lys Asn Ala  Leu Pro Ala Lys Asn  Ile Ala Lys 
    1175                 1180                 1185             


Ile Ser  Ile Asp Asp Ile Ser  Phe Pro Leu Lys Arg  Arg Pro Ile 
    1190                 1195                 1200             


Lys Ile  Asn Thr Leu Phe Glu  Phe Asp Gly Tyr Arg  Val Asn Ile 
    1205                 1210                 1215             


Arg Ser  Lys Asp Ser Tyr His  Tyr Phe Thr Ile Ser  Ser Ala Met 
    1220                 1225                 1230             


Ala Ala  Ile Tyr Ser Lys Asp  Thr Ser Asp Tyr Ile  Lys Ala Val 
    1235                 1240                 1245             


Ser Ser  Tyr Ile Asp Lys Ser  Asp Lys Gly Ser Lys  Phe Lys Pro 
    1250                 1255                 1260             


Gly Glu  Ala Phe Asp Val Leu  Ser Asn Leu Lys Val  Tyr Asp Glu 
    1265                 1270                 1275             


Ile Ala  Lys Lys Cys Ile Ser  Glu Pro Phe Ser Lys  Ile Ser Lys 
    1280                 1285                 1290             


Leu Ala  Glu Ala Gly Lys Arg  Trp Lys Lys Glu Glu  Ile Ser Leu 
    1295                 1300                 1305             


Gln Asn  Cys Leu Leu 
    1310             


<210>  814
<211>  1314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  814

Met Leu Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly His Ala Val Val Thr Asp Asp Tyr Lys Val Pro Thr Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asp Thr Ser Lys Lys Thr Ile Lys Lys Asn Met 
        35                  40                  45              


Leu Gly Val Leu Leu Phe Asn Glu Gly Gln Thr Ala Ala Asp Thr Arg 
    50                  55                  60                  


Leu Lys Arg Gly Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Pro Ala Leu Ala Lys Val Asp Pro 
                85                  90                  95      


Asn Phe Phe Tyr Arg Leu Glu Glu Ser Ser Leu Val Ala Glu Asp Lys 
            100                 105                 110         


Lys Tyr Asp Val Tyr Pro Ile Phe Gly Lys Arg Glu Glu Glu Leu Leu 
        115                 120                 125             


Tyr His Asp Thr His Lys Thr Ile Tyr His Leu Arg Ser Glu Leu Ala 
    130                 135                 140                 


Asn Asn Asp Arg Pro Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Leu Glu Gly Glu Ile Asp 
                165                 170                 175     


Leu Arg Thr Thr Asp Ile Asn Lys Val Phe Ala Glu Phe Ser Glu Thr 
            180                 185                 190         


Leu Asn Glu Asn Ser Asp Glu Asn Leu Gly Lys Leu Asp Val Ala Asp 
        195                 200                 205             


Ile Phe Lys Asp Asn Thr Phe Ser Lys Thr Lys Lys Ser Glu Glu Leu 
    210                 215                 220                 


Leu Lys Leu Ser Gly Ala Lys Lys Asn Gln Leu Ala His Gln Leu Phe 
225                 230                 235                 240 


Lys Met Met Val Gly Asn Met Gly Ser Phe Lys Lys Val Leu Gly Thr 
                245                 250                 255     


Asp Glu Glu His Lys Leu Ser Phe Gly Lys Asp Thr Tyr Glu Asp Asp 
            260                 265                 270         


Leu Asn Asp Leu Leu Ala Glu Ala Gly Asp Gln Tyr Leu Asp Ile Phe 
        275                 280                 285             


Val Ala Ala Lys Lys Val Tyr Asp Ala Ala Ile Leu Ala Ser Ile Leu 
    290                 295                 300                 


Asp Val Lys Asp Thr Gln Thr Lys Thr Val Phe Ser Gln Ala Met Ile 
305                 310                 315                 320 


Glu Arg Tyr Glu Glu His Gln Lys Asp Leu Ile Glu Leu Lys Arg Val 
                325                 330                 335     


Phe Lys Lys Tyr Leu Pro Glu Lys Cys His Asp Phe Phe Ser Glu Pro 
            340                 345                 350         


Lys Ile Ser Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Val Ser Glu Glu 
        355                 360                 365             


Asp Phe Tyr Lys Tyr Thr Lys Lys Thr Leu Lys Gly Ile Pro Glu Thr 
    370                 375                 380                 


Glu Glu Ile Leu Gln Lys Ile Asp Ala Asn Asn Tyr Leu Arg Lys Gln 
385                 390                 395                 400 


Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Val His Leu Lys Glu 
                405                 410                 415     


Leu Val Ala Ile Val Glu Asn Gln Gly Lys Tyr Tyr Pro Phe Leu Arg 
            420                 425                 430         


Glu Asn Lys Asp Lys Phe Glu Lys Ile Leu Asn Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Lys Phe Ala Trp Leu Thr 
    450                 455                 460                 


Arg Ala Gly Glu Gly Lys Ile Thr Pro Tyr Asn Phe Asp Glu Met Ile 
465                 470                 475                 480 


Asp Lys Glu Thr Ser Ala Glu Asp Phe Ile Lys Arg Met Thr Ile Asn 
                485                 490                 495     


Asp Leu Tyr Leu Pro Thr Glu Pro Val Leu Pro Lys His Ser Leu Leu 
            500                 505                 510         


Tyr Glu Arg Tyr Thr Ile Phe Asn Glu Leu Ala Gly Val Arg Tyr Val 
        515                 520                 525             


Thr Glu Asn Gly Glu Ala Lys Tyr Phe Asp Ala Gln Thr Lys Arg Ser 
    530                 535                 540                 


Ile Phe Glu Leu Phe Lys Leu Asp Arg Lys Val Ser Glu Lys Met Val 
545                 550                 555                 560 


Ile Lys His Leu Lys Val Val Met Pro Ala Ile Arg Ile Gln Ala Leu 
                565                 570                 575     


Lys Gly Leu Asp Asn Gly Lys Phe Asn Ala Ser Tyr Gly Thr Tyr Lys 
            580                 585                 590         


Asp Leu Val Asp Met Gly Val Ala Pro Glu Leu Leu Asn Asp Glu Val 
        595                 600                 605             


Asn Ser Glu Lys Trp Glu Asp Ile Ile Lys Thr Leu Thr Ile Phe Glu 
    610                 615                 620                 


Gly Arg Lys Leu Ile Lys Arg Arg Leu Glu Asn Tyr Arg Asp Phe Leu 
625                 630                 635                 640 


Gly Glu Asp Ile Leu Arg Lys Leu Ser Arg Lys Lys Tyr Thr Gly Trp 
                645                 650                 655     


Gly Arg Leu Ser Ala Lys Leu Leu Asp Gly Ile Tyr Asp Lys Lys Thr 
            660                 665                 670         


His Lys Thr Ile Leu Asp Cys Leu Met Thr Glu Asp Tyr Ser Gln Asn 
        675                 680                 685             


Phe Met Gln Leu Ile Asn Asp Asp Thr Tyr Ser Phe Lys Glu Thr Ile 
    690                 695                 700                 


Lys Asn Ala Gln Val Ile Glu Lys Glu Glu Thr Leu Ala Lys Thr Val 
705                 710                 715                 720 


Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Ser 
                725                 730                 735     


Leu Glu Ile Val Asp Glu Ile Ile Lys Val Met Gly Tyr Lys Pro Lys 
            740                 745                 750         


Ser Ile Val Val Glu Met Ala Arg Glu Thr Gln Lys Thr His Gly Thr 
        755                 760                 765             


Arg Lys Arg Glu Asp Arg Val Gln Gln Ile Val Lys Asn Leu Lys Asp 
    770                 775                 780                 


Ala Asn Glu Leu Pro Lys Lys Leu Pro Ser Asn Ala Glu Leu Ser Asp 
785                 790                 795                 800 


Glu Arg Lys Tyr Leu Tyr Cys Leu Gln Asn Gly Arg Asp Met Tyr Thr 
                805                 810                 815     


Gly Ala Pro Leu Asp Tyr Asp His Leu Gln Phe Tyr Asp Val Asp His 
            820                 825                 830         


Ile Ile Pro Gln Ser Phe Leu Lys Asp Asp Ser Ile Glu Asn Lys Val 
        835                 840                 845             


Leu Thr Ile Lys Lys Glu Asn Val Arg Lys Thr Asn Gly Leu Pro Ser 
    850                 855                 860                 


Glu Ala Val Ile Gln Lys Met Gly Ser Phe Trp Lys Lys Leu Leu Asp 
865                 870                 875                 880 


Ala Gly Ala Met Thr Asn Lys Lys Tyr Asp Asn Leu Arg Arg Asn Leu 
                885                 890                 895     


His Gly Gly Leu Asn Glu Lys Leu Lys Glu Arg Phe Ile Glu Arg Gln 
            900                 905                 910         


Leu Val Glu Thr Arg Gln Ile Thr Lys Tyr Val Ala Gln Leu Leu Asp 
        915                 920                 925             


Gln Arg Leu Asn Tyr Asp Gly Asn Gly Val Glu Leu Asp Glu Lys Ile 
    930                 935                 940                 


Ala Ile Val Thr Leu Lys Ala Gln Leu Ala Ser Gln Phe Arg Ser Glu 
945                 950                 955                 960 


Phe Lys Leu Arg Lys Val Arg Ala Leu Asn Asn Leu His His Ala His 
                965                 970                 975     


Asp Ala Tyr Leu Asn Ala Val Val Ala Asn Leu Ile Met Ala Lys Tyr 
            980                 985                 990         


Pro Glu Leu Glu Pro Glu Phe Val  Tyr Gly Lys Tyr Arg  Lys Thr Lys 
        995                 1000                 1005             


Phe Lys  Gly Leu Gly Lys Ala  Thr Ala Lys Asn Thr  Leu Tyr Ala 
    1010                 1015                 1020             


Asn Val  Leu Tyr Phe Leu Lys  Glu Asn Glu Val Tyr  Pro Phe Trp 
    1025                 1030                 1035             


Asp Lys  Ala Arg Asp Leu Pro  Thr Ile Lys Arg Tyr  Leu Tyr Arg 
    1040                 1045                 1050             


Ala Gln  Val Asn Lys Val Arg  Lys Ala Glu Arg Gln  Thr Gly Gly 
    1055                 1060                 1065             


Phe Ser  Asp Glu Met Leu Val  Pro Lys Ser Asp Ser  Gly Lys Leu 
    1070                 1075                 1080             


Leu Pro  Arg Lys Glu Gly Leu  Asp Pro Val Lys Tyr  Gly Gly Tyr 
    1085                 1090                 1095             


Ala Lys  Ala Val Glu Ser Tyr  Ala Val Leu Ile Thr  Ala Asp Glu 
    1100                 1105                 1110             


Val Lys  Lys Gly Lys Thr Lys  Lys Val Lys Thr Leu  Val Asn Ile 
    1115                 1120                 1125             


Pro Ile  Ile Asp Ser Lys Lys  Tyr Glu Ala Asp Pro  Thr Ala Tyr 
    1130                 1135                 1140             


Leu Ala  Ser Arg Gly Tyr Thr  Asn Val Thr Asn Ser  Phe Ile Leu 
    1145                 1150                 1155             


Pro Lys  Tyr Ser Leu Leu Glu  Asp Pro Glu Gly Arg  Arg Arg Tyr 
    1160                 1165                 1170             


Leu Ala  Ser Phe Lys Glu Phe  Gln Lys Ala Asn Glu  Leu Ile Leu 
    1175                 1180                 1185             


Pro Gln  His Leu Val Glu Leu  Leu Tyr Trp Val Asn  Ala Lys Asp 
    1190                 1195                 1200             


Gly Glu  Gln Lys Leu Glu Asp  His Lys Ala Glu Phe  Lys Glu Leu 
    1205                 1210                 1215             


Phe Asp  Lys Ile Met Glu Phe  Ala Asp Lys Tyr Val  Val Ala Pro 
    1220                 1225                 1230             


Lys Asn  Ser Glu Lys Ile Arg  Arg Leu Tyr Glu Glu  Asn Gln Asp 
    1235                 1240                 1245             


Ala Thr  Pro Met Glu Leu Gly  Lys Asn Phe Val Glu  Leu Leu Arg 
    1250                 1255                 1260             


Tyr Thr  Ala Asp Gly Ala Ala  Ser Asp Phe Lys Phe  Phe Gly Glu 
    1265                 1270                 1275             


Asn Ile  Pro Arg Lys Arg Tyr  Asn Ser Ala Gly Ser  Leu Leu Asn 
    1280                 1285                 1290             


Gly Thr  Leu Ile Tyr Gln Ser  Lys Thr Gly Leu Tyr  Glu Thr Arg 
    1295                 1300                 1305             


Ile Asp  Leu Gly Lys Leu 
    1310                 


<210>  815
<211>  1315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  815

Met Gly Tyr Ser Val Gly Leu Asp Ile Gly Thr Gly Ser Val Gly Trp 
1               5                   10                  15      


Val Val Thr Asn Glu Asp Gly Lys Leu Ala Arg Ala Lys Gly Lys Asn 
            20                  25                  30          


Leu Ile Gly Val Arg Leu Phe Lys Thr Ala Glu Pro Ala Ala Asp Arg 
        35                  40                  45              


Arg Thr Asn Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg Lys Trp Arg 
    50                  55                  60                  


Leu Arg Leu Leu Asp Glu Leu Phe Ala Asn Glu Leu Gly Lys Ile Asp 
65                  70                  75                  80  


Ser Asn Phe Leu Thr Arg Leu Lys Tyr Ser Tyr Val His Pro Lys Asp 
                85                  90                  95      


Asp Ala Asn Arg Ala Asn Tyr Tyr Gly Gly Tyr Leu Phe Pro Thr Gln 
            100                 105                 110         


Glu Glu Thr Lys Lys Phe His Ala Lys Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Leu Ala Leu Met Thr Glu Ser Arg Gln Phe Asp Leu Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Met His Gln Ile Val Lys Tyr Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Ser Gln Ser Lys Met Thr Ile Gly Asn Thr Tyr Asn Pro Gln Asp Leu 
                165                 170                 175     


Gln Thr Ala Met Glu Asn Tyr Ala Glu Ala Lys Gly Leu Ser Ile Glu 
            180                 185                 190         


Leu Lys Gln Pro Glu Glu Met Val Ala Ile Leu Thr Gly Glu Ala Gly 
        195                 200                 205             


Phe Gly Leu Ser Lys Lys Ala Lys Ala Glu Lys Leu Leu Asn Asn Phe 
    210                 215                 220                 


Phe Phe Asp Thr Lys Glu Glu Lys Ala Thr Val Gln Ala Ile Leu Ala 
225                 230                 235                 240 


Gly Ile Val Gly Asn Thr Ile Asp Phe Thr Lys Val Phe Asn Arg Gln 
                245                 250                 255     


Leu Thr Gly Asp Thr Leu Lys Ala Trp Lys Leu Lys Leu Asp Ser Glu 
            260                 265                 270         


Thr Phe Asp Val Asp Ser Glu Pro Ile Leu Ser Asp Leu Asp Asp Asp 
        275                 280                 285             


Glu Thr Glu Leu Phe Phe Ala Ile Arg Gln Ala Phe Asp Gly Phe Thr 
    290                 295                 300                 


Leu Met Asn Leu Leu Gly Asp Gln Thr Ser Ile Ser Ala Ala Met Val 
305                 310                 315                 320 


Glu Arg Tyr Thr Gln His His Asp Asp Leu Leu Thr Val Lys Gln Val 
                325                 330                 335     


Ala Lys Lys Gln Gly Leu Ser His Lys Glu Phe Ser Asn Ile Tyr Ser 
            340                 345                 350         


Ala Phe Leu Lys Asp Asp Thr Asp Lys Gly Met Lys Lys Leu Leu Glu 
        355                 360                 365             


Glu Ser Asn Leu Ser Thr Glu Asp Val Asn Gly Ile Gln Leu Arg Met 
    370                 375                 380                 


Asp Ser His Asp Phe Leu Pro Gln Gln Arg Thr Lys Ala Asn Ser Val 
385                 390                 395                 400 


Ile Pro His Gln Leu His Leu Ala Glu Leu Glu Ala Ile Ile Glu Asn 
                405                 410                 415     


Gln Gly Gln Tyr Tyr Pro Phe Leu Leu Glu Thr Phe Glu Lys Glu Gly 
            420                 425                 430         


Thr Ala Ala Asn Lys Ile Ala Glu Leu Leu Lys Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Met Val Thr Lys Lys Asp Val Glu Asn Ala Gly Gly 
    450                 455                 460                 


Asp Ala Thr Asn His Trp Val Thr Arg Asn Glu Gly Tyr Glu Lys Ser 
465                 470                 475                 480 


Ala Val Thr Ala Trp Asn Phe Asp Gln Val Phe Asn Arg Asp Gln Ala 
                485                 490                 495     


Ala Leu Asp Phe Ile Gln Arg Leu Thr Gly Thr Asp Thr Tyr Leu Ile 
            500                 505                 510         


Gly Glu Pro Thr Leu Pro Gln Asn Ser Leu Lys Tyr Gln Met Phe Thr 
        515                 520                 525             


Val Leu Asn Glu Leu Asn Asn Val Lys Val Asn Gly Arg Arg Ile Asp 
    530                 535                 540                 


Asn Gln Thr Lys His Asp Leu Ile Gln Asp Leu Phe Lys Ser Lys Lys 
545                 550                 555                 560 


Thr Val Ser Leu Lys Ala Phe Gln Asp Tyr Tyr Gln Ser Gln Gly Lys 
                565                 570                 575     


Ser Asp Ile Gln Val Val Gly Leu Ala Asp Lys Thr Lys Phe Asn Ser 
            580                 585                 590         


Asn Leu Ser Thr Tyr Ile Asp Leu Ser Lys Thr Phe Ser Ala Glu Glu 
        595                 600                 605             


Met Glu Asn Pro Gln Asn Gln Asp Leu Leu Glu Gln Ile Val Glu Ile 
    610                 615                 620                 


Gln Thr Val Phe Glu Asp Ser Lys Ile Ala Glu Arg Glu Leu Ser Lys 
625                 630                 635                 640 


Leu Glu Leu Thr Pro Glu Gln Val Thr Lys Leu Ala Lys Thr His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Asn Leu Ser Asn Lys Leu Leu Thr Thr Pro Ile Val 
            660                 665                 670         


Asp Val Ser Gly Lys Lys Val Ser Ile Leu Asp Gln Leu Ala Lys Thr 
        675                 680                 685             


Ser Lys Asn Phe Met Ser Ile Ile Ser Asn Glu Gln Tyr Gly Val Gln 
    690                 695                 700                 


Asp Trp Ile Lys Glu Gln Asn Thr Thr Glu Thr Thr Gly Thr Leu Glu 
705                 710                 715                 720 


Asp Lys Leu Ala Asp Leu Thr Thr Ser Pro Ala Asn Lys Arg Gly Ile 
                725                 730                 735     


Lys Gln Ala Phe Asn Val Leu Phe Asp Ile Gln Lys Ala Met Lys Ser 
            740                 745                 750         


Glu Pro Asp Arg Val Tyr Leu Glu Phe Ala Arg Glu Thr Gln Thr Ser 
        755                 760                 765             


Val Arg Thr Met Ser Arg Tyr Asn Tyr Leu Lys Gly Leu Tyr Gln Ser 
    770                 775                 780                 


Lys Ser Leu Asp Ala Glu Ala Lys Asn Leu Lys Lys Glu Leu Ala Glu 
785                 790                 795                 800 


Thr Asp Gln Lys Lys Phe Asp Asn Asp Arg Leu Tyr Leu Tyr Phe Gln 
                805                 810                 815     


Gln Gln Gly Lys Asp Met Tyr Thr Gly Gln Pro Ile Asn Ile Asp Lys 
            820                 825                 830         


Leu Ser Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Thr 
        835                 840                 845             


Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser Arg Pro Glu Asn 
    850                 855                 860                 


Ala Arg Lys Ser Asp Ser Gly Thr Tyr Thr Thr Glu Val Gln Ala Ala 
865                 870                 875                 880 


Ala Ser Gly Leu Trp Lys Thr Leu Val Gln Ser Gly Phe Ile Ser Arg 
                885                 890                 895     


Lys Lys Tyr Asp Arg Leu Thr His Ala Gly Asp Tyr Ser Glu Gly Gln 
            900                 905                 910         


Lys Thr Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
        915                 920                 925             


Lys Asn Val Ala Ala Leu Ile Glu Ser Glu Phe Asp Gln Thr Gln Ala 
    930                 935                 940                 


Val Ala Ile Arg Ala Glu Ile Thr Gly Asp Met Arg Arg Leu Val Ala 
945                 950                 955                 960 


Ile Lys Lys His Arg Glu Ile Asn Ser Phe His His Ala Phe Asp Ala 
                965                 970                 975     


Leu Leu Ile Thr Ala Ala Gly Gln Tyr Met Gln Lys Arg Tyr Pro Asp 
            980                 985                 990         


Arg Asp Ser Asn Asn Val Tyr Asn  Glu Phe Asp Arg Tyr  Thr Asn Asp 
        995                 1000                 1005             


Tyr Leu  Lys Lys Leu Arg Glu  Ser Ser Ser Lys Asp  Glu Val Arg 
    1010                 1015                 1020             


Lys Leu  Lys Pro Phe Gly Phe  Ile Val Gly Thr Met  Ala Lys Gly 
    1025                 1030                 1035             


Asn Glu  Asp Trp Thr Thr Glu  Gln Thr Glu Tyr Leu  Arg His Ile 
    1040                 1045                 1050             


Met Asn  Phe Lys Asn Ile Leu  Thr Thr Tyr Lys Thr  Gly Pro Asp 
    1055                 1060                 1065             


Arg Gly  Pro Leu Ser Lys Glu  Thr Ile Tyr Ala Val  Asp Pro Lys 
    1070                 1075                 1080             


Ala Lys  Leu Ile Gly Thr Asn  Lys His Arg Gln Asp  Val Ser Leu 
    1085                 1090                 1095             


Tyr Gly  Gly Tyr Thr Tyr Pro  Tyr Ser Ala Tyr Met  Thr Leu Val 
    1100                 1105                 1110             


Arg Ala  Asn Gly Lys Asn Gln  Leu Val Lys Val Ser  Arg Ser Ala 
    1115                 1120                 1125             


Ala Ser  Leu Met Lys Ser Gly  Gln Leu Lys Leu Glu  Asp Tyr Val 
    1130                 1135                 1140             


Gln Gln  Gln Pro Glu Val Lys  Lys Phe Glu Lys Ile  Leu Leu Glu 
    1145                 1150                 1155             


His Leu  Pro Leu Gly Gln Leu  Val Asp Asn Asp Gly  Lys Leu Met 
    1160                 1165                 1170             


Tyr Leu  Thr Ser Tyr Glu Phe  Tyr His Asn Ala Lys  Gln Leu Trp 
    1175                 1180                 1185             


Leu Ser  Thr Glu Asn Ala Asp  Leu Ile Ser Ser Leu  Ser Ile Asp 
    1190                 1195                 1200             


Ser Asp  Asp Gln Lys Leu Ile  Lys Ala Phe Asp Ile  Leu Thr Ala 
    1205                 1210                 1215             


Asp Ser  Val Ile Glu Arg Phe  Pro Phe Tyr Glu Ile  Asp Leu Lys 
    1220                 1225                 1230             


Lys Leu  Ile Glu Leu Arg Asp  Lys Phe Val Leu Ile  Asp Asn Lys 
    1235                 1240                 1245             


Leu Asp  Val Leu Arg Val Val  Leu Lys Ala Leu Gln  Leu Asp Ser 
    1250                 1255                 1260             


Ala Gln  Gln Lys Pro Leu Lys  Leu Ile Asp Lys Ala  Ala Thr Asp 
    1265                 1270                 1275             


Trp Lys  Asn Tyr Arg Gln Ala  Gly Gly Ile Lys Leu  Ser Asp Asn 
    1280                 1285                 1290             


Ala Glu  Ile Ile Tyr Gln Ser  Thr Thr Gly Ile Phe  Glu Lys Arg 
    1295                 1300                 1305             


Ile Lys  Ile Ser Asp Leu Leu  
    1310                 1315 


<210>  816
<211>  1316
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  816

Met Leu Gln Lys Asn Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Tyr Ala Val Ile Asn Asp Asn Tyr Lys Val Pro Ala Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Lys Thr Ile Lys Lys Asn Leu 
        35                  40                  45              


Met Gly Val Leu Leu Phe Asp Ala Gly Glu Thr Ala Ala Thr Thr Arg 
    50                  55                  60                  


Leu Lys Arg Gly Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Leu Gly Pro Glu Leu Ala Lys Val Asp Ala 
                85                  90                  95      


Asn Phe Leu His Arg Leu Ala Glu Ser Ser Leu Val Thr Glu Asp Lys 
            100                 105                 110         


Glu Phe Ser Ala Tyr Pro Ile Phe Gly Asp Arg Glu Glu Glu Leu Leu 
        115                 120                 125             


Tyr His Lys Thr Tyr Lys Thr Ile Tyr His Leu Arg Ser Ala Leu Ala 
    130                 135                 140                 


Asn Lys Asp Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Glu Ile Glu 
                165                 170                 175     


Leu Ser Asp Thr Asp Ile Asp Lys Val Tyr Ala Asp Phe Cys Arg Ser 
            180                 185                 190         


Phe Asn Glu Leu Phe Asp Thr Glu Val Leu Gln Glu Ile Glu Val Glu 
        195                 200                 205             


Ala Ile Phe Lys Asp Lys Thr Leu Ser Lys Thr Lys Lys Ala Glu Glu 
    210                 215                 220                 


Leu Val Lys Val Ser Gly Val Lys Lys Asn Gln Val Ala His Gln Phe 
225                 230                 235                 240 


Leu Lys Met Met Val Gly Asn Thr Val Asn Phe Lys Lys Val Phe Asn 
                245                 250                 255     


Leu Thr Glu Asp Gln Lys Leu Ser Phe Gly Thr Asp Ser Tyr Glu Asp 
            260                 265                 270         


Glu Leu Asn Asp Leu Leu Ala Lys Ser Asp Asp Arg Tyr Leu Asp Val 
        275                 280                 285             


Phe Val Ala Ala Lys Lys Val Tyr Asp Thr Ala Ile Leu Ala Asn Ile 
    290                 295                 300                 


Leu Asn Thr Lys Asn Ser Val Ala Thr Lys Thr Ile Phe Ser Gln Ser 
305                 310                 315                 320 


Met Ile Glu Arg Phe Lys Asp His Gln Lys Asp Leu Lys Glu Leu Lys 
                325                 330                 335     


Gln Leu Phe Lys Thr Tyr Leu Pro Glu Lys Tyr Pro Asp Phe Phe Gly 
            340                 345                 350         


Asn Ser Lys Glu Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Asn 
        355                 360                 365             


Glu Glu Lys Phe Tyr Thr His Thr Lys Lys Ile Leu Lys Asn Ile Pro 
    370                 375                 380                 


Gln Ala Ala Ser Val Leu Ala Gln Ile Asp Val Gly Asn Tyr Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile Gln Leu 
                405                 410                 415     


Lys Glu Leu Leu Ala Ile Ile Glu Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         


Leu Leu Glu Asn Lys Glu Lys Ile Ala Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Leu Lys Arg Val Gly Lys Glu Lys Ile Thr Pro Phe Asn Phe Glu Asn 
465                 470                 475                 480 


Val Val Asp Leu Glu Ser Ser Ala Glu Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Met Leu Tyr Glu Lys Phe Ser Ile Phe Asn Glu Leu Ala Gly Ile Arg 
        515                 520                 525             


Tyr Ile Thr Asp Asn Gly Glu Met Lys Phe Leu Asp Ala Gln Thr Lys 
    530                 535                 540                 


Gln Ser Ile Phe Glu Leu Phe Lys Gln Glu Arg Thr Val Lys Glu Lys 
545                 550                 555                 560 


Lys Val Leu Asp Leu Leu Lys Thr Leu Met Pro Ser Glu Arg Val Val 
                565                 570                 575     


Ala Ile Lys Gly Ile Asp Asn Gly Lys Phe Asn Ala Ser Phe Gly Thr 
            580                 585                 590         


Tyr His Asp Leu Ser Lys Met Gly Phe Lys Thr Glu Lys Ile Ala Ser 
        595                 600                 605             


Glu Ser Asn Gln Ala Arg Trp Glu Glu Ile Ile Lys Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Lys Leu Ile Lys Ser Arg Leu Arg Lys Tyr Asp Asp 
625                 630                 635                 640 


Phe Leu Asn Glu Lys Glu Ile Gly Ala Leu Ser Lys Arg Lys Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asp Gly Ile Tyr Asp Lys 
            660                 665                 670         


Lys Thr His Gln Thr Ile Leu Asp Phe Leu Met Asn Glu Asp Tyr Asn 
        675                 680                 685             


Gln Asn Phe Met Gln Leu Ile Ser Asn Asp Asn Tyr Ser Phe Lys Lys 
    690                 695                 700                 


Ile Ile Glu Asp Ser Gln Val Ile Lys Glu Glu Gly Ser Leu Thr Glu 
705                 710                 715                 720 


Val Ile Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu 
                725                 730                 735     


Gln Ser Ile Glu Ile Val Asp Glu Leu Val Lys Val Met Gly Gly Asn 
            740                 745                 750         


Pro Lys Lys Ile Val Val Glu Met Ala Arg Glu Thr Gln Lys Thr His 
        755                 760                 765             


Gly Thr Thr Arg Arg Glu Ala Arg Ile Lys Glu Ile Ile Lys Asn Leu 
    770                 775                 780                 


Asn Lys Leu Lys Leu Asn Glu Leu Pro Lys Asp Leu Pro Ser Asn Glu 
785                 790                 795                 800 


Glu Leu Ser Asp Glu Lys Val Tyr Leu Tyr Cys Leu Gln Asn Gly Arg 
                805                 810                 815     


Asp Met Tyr Thr Gly Ala Pro Leu Asp Leu Asp Asn Leu Ser Gln Tyr 
            820                 825                 830         


Glu Val Asp His Ile Ile Pro Gln Ser Phe Leu Lys Asp Asp Ser Ile 
        835                 840                 845             


Glu Asn Lys Val Leu Thr Ala Lys Ile Glu Asn Ser Arg Lys Thr Asn 
    850                 855                 860                 


Gly Leu Pro Ser Val Glu Ile Ala Lys Lys Met Gly Ala Phe Trp Arg 
865                 870                 875                 880 


Ser Leu Leu Lys Val Gly Ala Ile Ser Glu Lys Lys Tyr Arg Asn Leu 
                885                 890                 895     


Arg Arg Ser Leu His Gly Gly Met Thr Glu Lys Leu Lys Glu Thr Phe 
            900                 905                 910         


Val Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Tyr Val Ala 
        915                 920                 925             


Gln Ile Leu Asn Ser Arg Phe Asn Glu Glu Lys Asn Glu Asp Lys Val 
    930                 935                 940                 


Ala Ile Val Thr Leu Lys Ala Gln Leu Thr Ser Gln Phe Arg Lys Asp 
945                 950                 955                 960 


Phe Glu Leu Tyr Lys Val Arg Glu Ile Asn Asn Leu His His Ala His 
                965                 970                 975     


Asp Ala Tyr Leu Asn Ala Val Leu Ala Thr Leu Leu Met Thr Ser Phe 
            980                 985                 990         


Pro Glu Leu Glu Pro Glu Phe Val  Tyr Gly Lys Tyr Arg  Lys Thr Lys 
        995                 1000                 1005             


Phe Lys  Gly Leu Gly Lys Ala  Thr Ala Lys Asn Asn  Leu Tyr Thr 
    1010                 1015                 1020             


Asn Ile  Leu Gln Leu Leu Lys  Glu Glu Ser Ala Gln  Tyr Trp Asp 
    1025                 1030                 1035             


Thr Lys  Glu Arg Asp Leu Ala  Thr Ile Lys Lys Asn  Val Tyr Gln 
    1040                 1045                 1050             


Ala Gln  Val Asn Lys Val Arg  Lys Val Glu Arg Gln  Thr Gly Ser 
    1055                 1060                 1065             


Phe Tyr  Asp Glu Thr Val Ala  Lys Lys Ser Ala Ser  Gln Lys Leu 
    1070                 1075                 1080             


Phe Pro  Arg Lys Asn Gly Leu  Asp Pro Val Lys Tyr  Gly Gly Tyr 
    1085                 1090                 1095             


Gln Arg  Ala Asn Glu Gly Tyr  Ser Leu Leu Phe Arg  Ala Asp Met 
    1100                 1105                 1110             


Leu Asn  Lys Arg Gly Lys Thr  Lys Ser Val Lys Thr  Leu Val Lys 
    1115                 1120                 1125             


Ile Pro  Ile Ile Glu Ser Ala  Gln Tyr Leu Ala Asp  Pro Thr Glu 
    1130                 1135                 1140             


Tyr Leu  Ala Lys Arg Gly Tyr  Tyr Asn Val Ser Glu  His Ser Leu 
    1145                 1150                 1155             


Leu Leu  Pro Lys Phe Ser Leu  Leu Glu Asn Asn Asn  Gly Leu Arg 
    1160                 1165                 1170             


Arg Tyr  Leu Ala Ser Ser Asn  Glu Phe Gln Lys Ala  Asn Glu Leu 
    1175                 1180                 1185             


Ile Leu  Pro Lys His Leu Val  Arg Leu Leu Tyr Trp  Ser Gln Ser 
    1190                 1195                 1200             


Lys Gln  Arg Lys Glu Glu Leu  Asn Lys His Arg Ala  Glu Phe Ala 
    1205                 1210                 1215             


Glu Leu  Phe Thr Tyr Ile Met  Asp Phe Ala Asp Lys  Tyr Val Val 
    1220                 1225                 1230             


Ala Pro  Lys Asn Ser Glu Lys  Ile Lys Asn Ile Tyr  Glu Asn Asn 
    1235                 1240                 1245             


Lys Asp  Ser Ser Val Glu Val  Leu Gly Lys Asn Phe  Ile Glu Leu 
    1250                 1255                 1260             


Leu Lys  Tyr Thr Ala Asp Gly  Ala Ala Ser Asp Phe  Thr Phe Phe 
    1265                 1270                 1275             


Asp Thr  Lys Ile Pro Arg Lys  Arg Tyr Asn Ser Ala  Gly Asp Leu 
    1280                 1285                 1290             


Leu Asp  Gly Thr Leu Ile Tyr  Gln Ser Lys Thr Gly  Leu Tyr Glu 
    1295                 1300                 1305             


Thr Arg  Ile Asp Leu Gly Ala  Ile 
    1310                 1315     


<210>  817
<211>  1318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  817

Met Met Ile Lys Leu Asn Asn Met Lys Lys Ile Leu Gly Leu Asp Leu 
1               5                   10                  15      


Gly Thr Asn Ser Ile Gly Trp Ala Val Val Glu Thr Glu Asp Asn Lys 
            20                  25                  30          


Lys Phe Glu Leu Leu Asp Lys Gly Val Arg Ile Phe Gln Glu Gly Val 
        35                  40                  45              


Lys Ile Glu Lys Gly Ile Glu Ser Ser Lys Ala Ala Glu Arg Thr Gly 
    50                  55                  60                  


Tyr Arg Ser Ala Arg Arg Ile Lys Phe Arg Arg Arg Leu Arg Lys Ile 
65                  70                  75                  80  


Glu Thr Leu Lys Val Leu Ile Glu Asn Asn Met Cys Pro Leu Ser Met 
                85                  90                  95      


Asp Glu Leu Ile Val Trp Lys Glu Asn Lys Asn Asn Tyr Pro Lys Asn 
            100                 105                 110         


Lys Ile Phe Tyr Asn Trp Leu Lys Thr Lys Asp Pro Lys Glu Gly Thr 
        115                 120                 125             


Asn Gly Thr Tyr Ile Asn Pro Tyr Tyr Phe Arg Thr Glu Ala Ile Lys 
    130                 135                 140                 


Arg Lys Leu Glu Asn Thr Asn Asp Val Gly Arg Ala Leu Tyr His Leu 
145                 150                 155                 160 


Ala Gln Arg Arg Gly Phe Lys Ser Asn Arg Leu Glu Ser Thr Asp Glu 
                165                 170                 175     


Lys Ser Arg Gly Ile Val Lys Thr Glu Ile Asn Lys Leu Ser Ser Glu 
            180                 185                 190         


Leu Asn Gly Arg Tyr Leu Gly Glu Tyr Phe Tyr Glu Leu Tyr Leu Lys 
        195                 200                 205             


Gly Glu Lys Ile Arg Lys Arg His Ile Ser Arg Glu Glu His Tyr Leu 
    210                 215                 220                 


Asn Glu Phe Lys Lys Ile Cys Glu Val Gln Asn Phe Asn Lys Glu Leu 
225                 230                 235                 240 


Ala Glu Lys Leu Glu Arg Ala Ile Phe Tyr Gln Arg Pro Leu Lys Ser 
                245                 250                 255     


Gln Lys Gly Leu Val Gly Lys Cys Thr Leu Gln Asn Asn Lys His Arg 
            260                 265                 270         


Cys Pro Ile Ser Arg Pro Glu Phe Glu Glu Phe Arg Met Trp Gln Phe 
        275                 280                 285             


Ile Asn Ser Ile Lys Tyr Lys Asn Ser Asp Gly Lys Phe Asp Phe Leu 
    290                 295                 300                 


Asn Lys Glu Gln Arg Glu Asn Ile Ile Pro Ala Phe Val Ser Asn Lys 
305                 310                 315                 320 


Asn Ala Thr Phe Glu Phe Ser Lys Ile Glu Lys Ile Leu Asn Lys Gly 
                325                 330                 335     


Val His Ile Tyr Gln Phe Asn Tyr Lys Ser Asn His Thr Ile Gly Ser 
            340                 345                 350         


Asn Gln Thr Met Ile Asn Phe Met Asp Val Phe Asp Ile Asp Arg Lys 
        355                 360                 365             


Leu Thr Asn Ala Trp Leu Leu Cys Val Asn Lys Ile Tyr Glu Leu Tyr 
    370                 375                 380                 


Thr Ala Lys Thr Lys Lys Asp Gly Ser Leu Lys Ser Lys Ser Glu Val 
385                 390                 395                 400 


Ile Thr Asp Val Trp His Val Leu Phe Thr Phe Asp Asn Glu Glu Asn 
                405                 410                 415     


Leu Lys Glu Phe Ala Arg Asn Lys Leu Leu Leu Asp Glu Ser Ser Ala 
            420                 425                 430         


Gln Lys Phe Ser Lys Ile Asn Leu Lys Gln Asp Tyr Ser Gln Leu Ser 
        435                 440                 445             


Leu Lys Ala Ile Asn Lys Ile Leu Pro Tyr Leu Gln Glu Gly Leu Ile 
    450                 455                 460                 


Tyr Ser Tyr Ala Val Phe Ile Ala Asn Ile Glu Asn Val Ile Gly Lys 
465                 470                 475                 480 


Ser Ile Trp Asn Ile Lys Glu Asn Gln Gln Ile Ile Arg Thr Ser Ile 
                485                 490                 495     


Lys Lys Ile Ile Asp Asn His Lys Thr Glu Leu Asp Ile Asn Thr Ile 
            500                 505                 510         


Thr Asn Ser Leu Ile Asn Asp Phe Lys Val Asn Tyr Asn Asn Ala His 
        515                 520                 525             


Lys Glu Tyr Lys Val Asp Asp Ile Asp Leu Lys Asn Ile Arg Glu Lys 
    530                 535                 540                 


Ile Ile Lys Tyr Tyr Gly Asn Lys Thr Trp Asn Cys Leu Pro Glu Asp 
545                 550                 555                 560 


Glu Lys Leu Tyr Val Lys Glu Lys Val Glu Lys Leu Tyr Glu Lys Gln 
                565                 570                 575     


Leu Lys Ser Gly Lys Arg Gly Ile Phe Ile Glu Lys Ile Arg Leu Asp 
            580                 585                 590         


Asp Arg Ile Glu Asn Phe Leu Lys Asp Asn Phe Asp Ser Ser Glu Leu 
        595                 600                 605             


Asn Leu Lys Lys Leu Tyr His Pro Ser Asp Val Ser Val Tyr Lys Ser 
    610                 615                 620                 


Val Lys Ile Thr Asp Leu Gly Leu Arg Leu Leu Gly Ser Pro Met Ile 
625                 630                 635                 640 


Pro Ser Ile Lys Asn Pro Met Ala Met Arg Ala Met His Gln Leu Arg 
                645                 650                 655     


Lys Leu Ile Asn His Leu Ile Ile Glu Gly Val Ile Asp Asp Ser Thr 
            660                 665                 670         


Ile Ile Asn Val Glu Met Ser Arg Glu Leu Asn Asp Ala Asn Met Arg 
        675                 680                 685             


Lys Ala Ile Glu Arg Tyr Gln Arg Gln Arg Ala Thr Glu Asn Glu Glu 
    690                 695                 700                 


Phe Lys Thr Lys Ile Lys Glu Leu Tyr Lys Lys Glu Cys Gly Thr Asp 
705                 710                 715                 720 


Phe Glu Pro Asn Asn Asp Asp Ile Met Lys Tyr Arg Leu Trp Glu Glu 
                725                 730                 735     


Gln Asn His Lys Cys Leu Tyr Thr Gly Lys Ser Ile Gly Ile Cys Asp 
            740                 745                 750         


Phe Ile Gly Ser Asn Pro Ala Phe Glu Ile Glu His Thr Ile Pro Arg 
        755                 760                 765             


Ser Leu Ser Tyr Asp Asn Ser Leu Glu Asn Lys Thr Leu Cys Glu Lys 
    770                 775                 780                 


Glu Phe Asn Gln Gln Val Lys Lys Asn Arg Ile Pro Phe Glu Leu Ser 
785                 790                 795                 800 


Asn Tyr Lys Glu Ile Ile Ser Arg Ile Asn Asn Trp Glu Ile Lys Ala 
                805                 810                 815     


Glu Glu Leu Phe Lys Gln Ile Gln Lys Val Lys Ile Gln Ser Arg Met 
            820                 825                 830         


Ala Ala Thr Lys Glu Ile Lys Asp Asn Ala Ile Gln Arg Lys His Leu 
        835                 840                 845             


Leu Gln Met Glu Phe Asn Tyr Trp Asn Gly Lys Leu Ser Arg Phe Thr 
    850                 855                 860                 


Arg Glu Asp Val Pro Glu Gly Phe Lys Asn Ser Gln Leu Val Asp Ile 
865                 870                 875                 880 


Gly Ile Ile Thr Lys Tyr Ala Arg Ala Tyr Leu Lys Thr Val Phe Asn 
                885                 890                 895     


Asn Val Asn Thr Val Lys Gly Ser Thr Val Ala Glu Phe Arg Asn Ile 
            900                 905                 910         


Trp Gly Leu Gln Glu Tyr Phe Glu Lys Lys Asp Arg Ser Asn His Ile 
        915                 920                 925             


His His Cys Ile Asp Ala Ile Thr Ile Ala Cys Leu Thr Lys Asp Lys 
    930                 935                 940                 


Tyr Asp Phe Leu Ala His Leu Tyr His Leu Asp Glu Gln Asn Asn Arg 
945                 950                 955                 960 


Asp Ala Val His Arg Ala Met Ile Lys Ser Lys Pro Trp Glu Asn Phe 
                965                 970                 975     


Val Gln Asp Val Lys Glu Ile Glu Asn Gln Ile Leu Val Ser His Tyr 
            980                 985                 990         


Thr Pro Asp Asn Leu Pro Lys Gln  Ser Lys Lys Ile Leu  Arg Lys Arg 
        995                 1000                 1005             


Gly Lys  Ile Gln Lys Asn Glu  Asn Gly Asp Leu Ile  Tyr Glu Lys 
    1010                 1015                 1020             


Gly Asp  Ala Val Arg Gly Ser  Leu His Lys Glu Ser  Phe Tyr Gly 
    1025                 1030                 1035             


Ala Ile  Lys Arg Glu Ile Ile  Asn Lys Lys Gly Glu  Thr Glu Glu 
    1040                 1045                 1050             


Lys Val  Leu Tyr Val Lys Arg  Lys Pro Leu Glu Phe  Ser Asp Leu 
    1055                 1060                 1065             


Gly Phe  Lys Thr Ile Ser Glu  Leu Glu Asn Ile Val  Asp Asp Ala 
    1070                 1075                 1080             


Val Arg  Asp Lys Val Leu Asn  Ala Val Lys Asp Lys  Gly Leu Lys 
    1085                 1090                 1095             


Gln Ala  Leu Asn Glu Pro Ile  Trp Met Asn Glu Glu  Lys Gln Ile 
    1100                 1105                 1110             


Pro Ile  Lys Lys Val Arg Cys  Tyr Thr Pro Ser Val  Thr Asn Pro 
    1115                 1120                 1125             


Ile Asn  Leu Lys Lys His Arg  Asp Lys Ser Lys His  Glu Tyr Lys 
    1130                 1135                 1140             


Gln Ser  Tyr His Val Thr Asn  Asp Gly Asn Tyr Leu  Met Val Ile 
    1145                 1150                 1155             


Tyr Glu  Gly Lys Asp Ser Gln  Glu Lys Val Lys Arg  Asp Tyr Glu 
    1160                 1165                 1170             


Ile Val  Ser Asn Leu Gln Ala  Gly Glu Tyr Phe Lys  Gln Ser Val 
    1175                 1180                 1185             


His Gly  Val Leu Lys Ala Gln  Gly Ile Asn Asn Leu  Glu Gly Leu 
    1190                 1195                 1200             


Ile Pro  Lys Phe Lys Glu Ser  Gly Lys Asn Ile Leu  Pro Leu Lys 
    1205                 1210                 1215             


Tyr Ile  Leu Lys Thr Gly Thr  Met Val Ile Phe Trp  Glu Asn Tyr 
    1220                 1225                 1230             


Pro Glu  Glu Ile Trp Asp Ile  Asp Lys Asn Gln Leu  Tyr Lys Arg 
    1235                 1240                 1245             


Leu Phe  Lys Val Thr Gln Phe  Glu Ser Asp Gly Arg  Ile Gln Ala 
    1250                 1255                 1260             


Arg Phe  His Gln Ile Ala Lys  Pro Asp Asn Glu Leu  Thr Lys Val 
    1265                 1270                 1275             


Ser Glu  Leu Asp Phe Ser Asn  Ile Ala Glu Lys Ile  Arg Leu Ser 
    1280                 1285                 1290             


Lys Gly  Asn Leu Asn Met Leu  Val Asn Gly Tyr Asp  Phe Lys Ile 
    1295                 1300                 1305             


Asn Val  Leu Gly His Ile Glu  Lys Leu Ser 
    1310                 1315             


<210>  818
<211>  1318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  818

Met Gly Ser Asn Met Leu Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile 
1               5                   10                  15      


Gly Thr Asn Ser Val Gly His Ala Val Val Thr Asp Asp Tyr Lys Val 
            20                  25                  30          


Pro Thr Lys Lys Met Lys Val Phe Gly Asp Thr Ser Lys Lys Thr Ile 
        35                  40                  45              


Lys Lys Asn Met Leu Gly Val Leu Leu Phe Asn Glu Gly Gln Thr Ala 
    50                  55                  60                  


Ala Asp Thr Arg Leu Lys Arg Gly Thr Arg Arg Arg Tyr Thr Arg Arg 
65                  70                  75                  80  


Lys Asn Arg Leu Arg Tyr Leu Gln Glu Ile Phe Ala Pro Glu Leu Ala 
                85                  90                  95      


Lys Val Asp Pro Asn Phe Leu Tyr Arg Leu Glu Glu Ser Ser Leu Val 
            100                 105                 110         


Ala Glu Asp Lys Lys Tyr Asp Val Tyr Pro Ile Phe Gly Lys Arg Glu 
        115                 120                 125             


Glu Glu Leu Leu Tyr His Asp Thr Tyr Lys Thr Ile Tyr His Leu Arg 
    130                 135                 140                 


Ser Ala Leu Ala Asn Asn Asp Gln Pro Ala Asp Leu Arg Leu Val Tyr 
145                 150                 155                 160 


Leu Ala Leu Ala His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Leu Glu 
                165                 170                 175     


Gly Glu Ile Asp Leu Arg Thr Thr Asp Ile Asn Lys Val Phe Ala Glu 
            180                 185                 190         


Phe Ser Glu Thr Leu Asn Glu Asn Ser Asp Glu Asn Leu Gly Lys Leu 
        195                 200                 205             


Asp Val Ala Asp Ile Phe Lys Asn Asn Thr Phe Ser Lys Thr Lys Lys 
    210                 215                 220                 


Ser Glu Glu Leu Leu Lys Leu Ser Gly Ala Lys Lys Asn Gln Leu Ala 
225                 230                 235                 240 


His Gln Leu Phe Lys Met Met Val Gly Asn Met Gly Ser Phe Lys Lys 
                245                 250                 255     


Val Leu Gly Thr Asp Glu Glu His Lys Leu Ser Phe Gly Lys Asp Thr 
            260                 265                 270         


Tyr Glu Asp Asp Leu Asn Asp Leu Leu Ala Glu Ala Gly Asp Gln Tyr 
        275                 280                 285             


Leu Asp Ile Phe Val Ala Ala Lys Lys Val Tyr Asp Ala Ala Ile Leu 
    290                 295                 300                 


Ala Ser Ile Leu Asp Val Lys Asp Thr Gln Thr Lys Thr Val Phe Ser 
305                 310                 315                 320 


Gln Ala Met Ile Glu Arg Tyr Glu Glu His Gln Lys Asp Leu Ile Glu 
                325                 330                 335     


Leu Lys Arg Val Phe Lys Lys Tyr Leu Pro Glu Lys Cys His Asp Phe 
            340                 345                 350         


Phe Ser Glu Pro Lys Ile Ser Gly Tyr Ala Gly Tyr Ile Asp Gly Lys 
        355                 360                 365             


Val Ser Glu Glu Asp Phe Tyr Lys Tyr Thr Lys Lys Thr Leu Lys Gly 
    370                 375                 380                 


Ile Pro Glu Thr Glu Glu Ile Leu Gln Lys Ile Asp Ala Asn Asn Tyr 
385                 390                 395                 400 


Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Val 
                405                 410                 415     


His Leu Lys Glu Leu Val Ala Ile Val Glu Asn Gln Gly Lys Tyr Tyr 
            420                 425                 430         


Pro Phe Leu Arg Glu Asn Lys Asp Lys Phe Glu Lys Ile Leu Asn Phe 
        435                 440                 445             


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Lys Phe 
    450                 455                 460                 


Ala Trp Leu Thr Arg Ala Gly Glu Gly Lys Ile Thr Pro Tyr Asn Phe 
465                 470                 475                 480 


Asp Glu Met Ile Asp Lys Glu Thr Ser Ala Glu Asp Phe Ile Lys His 
                485                 490                 495     


Met Thr Ile Asn Asp Leu Tyr Leu Pro Thr Glu Pro Val Leu Pro Lys 
            500                 505                 510         


His Ser Leu Leu Tyr Glu Arg Tyr Thr Ile Phe Asn Glu Leu Ala Gly 
        515                 520                 525             


Val Arg Tyr Val Thr Glu Asn Gly Glu Ala Lys Tyr Phe Asp Ala Gln 
    530                 535                 540                 


Thr Lys Arg Ser Ile Phe Glu Leu Phe Lys Leu Asp Arg Lys Val Ser 
545                 550                 555                 560 


Glu Lys Met Val Ile Lys His Leu Lys Val Val Met Pro Ala Ile Arg 
                565                 570                 575     


Ile Gln Ala Leu Lys Gly Leu Asp Asn Gly Lys Phe Asn Ala Ser Tyr 
            580                 585                 590         


Gly Thr Tyr Lys Asp Leu Val Asp Met Gly Val Ala Pro Glu Leu Leu 
        595                 600                 605             


Asn Asp Glu Val Asn Ser Glu Lys Trp Glu Asp Ile Ile Lys Thr Leu 
    610                 615                 620                 


Thr Ile Phe Glu Gly Arg Lys Leu Ile Lys Arg Arg Leu Glu Asn Tyr 
625                 630                 635                 640 


Arg Asp Phe Leu Gly Glu Asp Ile Leu Arg Lys Leu Ser Arg Lys Lys 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Asp Gly Ile Tyr 
            660                 665                 670         


Asp Lys Lys Thr His Lys Thr Ile Leu Asp Cys Leu Met Thr Glu Asp 
        675                 680                 685             


Tyr Ser Gln Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Tyr Ser Phe 
    690                 695                 700                 


Lys Glu Thr Ile Lys Asn Ala Gln Val Ile Glu Lys Glu Glu Thr Leu 
705                 710                 715                 720 


Ala Lys Thr Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Leu Glu Ile Val Asp Glu Ile Ile Lys Val Met Gly 
            740                 745                 750         


Tyr Lys Pro Lys Ser Ile Val Val Glu Met Ala Arg Glu Thr Gln Lys 
        755                 760                 765             


Thr His Gly Thr Arg Lys Arg Glu Asp Arg Val Gln Gln Ile Val Glu 
    770                 775                 780                 


Asn Leu Lys Asp Ala Asn Glu Leu Pro Glu Lys Leu Pro Ser Asn Ala 
785                 790                 795                 800 


Glu Leu Ser Asp Glu Arg Lys Tyr Leu Tyr Cys Leu Gln Asn Gly Arg 
                805                 810                 815     


Asp Met Tyr Thr Gly Ala Pro Leu Asp Tyr Asp His Leu Gln Phe Tyr 
            820                 825                 830         


Asp Val Asp His Ile Ile Pro Gln Ser Phe Leu Lys Asp Asp Ser Ile 
        835                 840                 845             


Glu Asn Lys Val Leu Thr Ile Lys Lys Glu Asn Val Arg Lys Thr Asn 
    850                 855                 860                 


Gly Leu Pro Ser Glu Ala Val Ile Gln Lys Met Gly Ser Phe Trp Lys 
865                 870                 875                 880 


Lys Leu Leu Asp Ala Gly Ala Met Thr Asn Lys Lys Tyr Asp Asn Leu 
                885                 890                 895     


Arg Arg Asn Leu His Gly Gly Leu Asn Glu Lys Leu Lys Glu Arg Phe 
            900                 905                 910         


Ile Glu Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Tyr Val Ala 
        915                 920                 925             


Gln Leu Leu Asp Gln Arg Leu Asn Tyr Asp Gly Asn Gly Val Glu Leu 
    930                 935                 940                 


Asp Glu Lys Ile Ala Ile Val Thr Leu Lys Ala Gln Leu Ala Ser Gln 
945                 950                 955                 960 


Phe Arg Ser Glu Phe Lys Leu Arg Lys Val Arg Ala Leu Asn Asn Leu 
                965                 970                 975     


His His Ala His Asp Ala Tyr Leu Asn Ala Ile Val Ala Asn Leu Ile 
            980                 985                 990         


Met Ala Lys Tyr Pro Glu Leu Glu  Pro Glu Phe Val Tyr  Gly Lys Tyr 
        995                 1000                 1005             


Arg Lys  Ala Lys Phe Lys Gly  Leu Gly Lys Ala Thr  Ala Lys Asn 
    1010                 1015                 1020             


Thr Leu  Tyr Ala Asn Val Leu  Tyr Phe Leu Lys Glu  Asn Glu Val 
    1025                 1030                 1035             


Tyr Pro  Phe Trp Asp Lys Ala  Arg Asp Leu Pro Thr  Ile Lys Arg 
    1040                 1045                 1050             


Tyr Leu  Tyr Arg Ala Gln Val  Asn Lys Val Arg Lys  Ala Glu Arg 
    1055                 1060                 1065             


Gln Ile  Gly Gly Phe Ser Asp  Glu Met Leu Val Pro  Lys Ser Asp 
    1070                 1075                 1080             


Ser Gly  Lys Leu Leu Pro Arg  Lys Glu Gly Leu Asp  Pro Val Lys 
    1085                 1090                 1095             


Tyr Gly  Gly Tyr Ala Lys Ala  Val Glu Ser Tyr Ala  Val Leu Ile 
    1100                 1105                 1110             


Thr Ala  Asp Glu Val Lys Lys  Gly Lys Thr Lys Lys  Val Lys Thr 
    1115                 1120                 1125             


Leu Val  Asn Ile Pro Ile Ile  Asp Ser Lys Lys Tyr  Glu Ala Asp 
    1130                 1135                 1140             


Pro Thr  Ala Tyr Leu Ala Ser  Arg Gly Tyr Thr Asn  Val Thr Asn 
    1145                 1150                 1155             


Ser Phe  Ile Leu Pro Lys Tyr  Ser Leu Leu Glu Asp  Pro Glu Gly 
    1160                 1165                 1170             


Arg Arg  Arg Tyr Leu Ala Ser  Phe Lys Glu Phe Gln  Lys Ala Asn 
    1175                 1180                 1185             


Glu Leu  Ile Leu Pro Gln His  Leu Val Glu Leu Leu  Tyr Trp Val 
    1190                 1195                 1200             


Asn Ala  Lys Asp Gly Glu Gln  Lys Leu Glu Asp His  Lys Ala Glu 
    1205                 1210                 1215             


Phe Lys  Glu Leu Phe Asp Lys  Ile Met Glu Phe Ala  Asp Lys Tyr 
    1220                 1225                 1230             


Val Val  Ala Pro Lys Asn Ser  Glu Lys Ile Arg Arg  Leu Tyr Glu 
    1235                 1240                 1245             


Glu Asn  Gln Asp Ala Thr Pro  Met Glu Leu Gly Lys  Asn Phe Val 
    1250                 1255                 1260             


Glu Leu  Leu Arg Tyr Thr Ala  Asp Gly Ala Ala Ser  Asp Phe Lys 
    1265                 1270                 1275             


Phe Phe  Gly Glu Asn Ile Pro  Arg Lys Arg Tyr Asn  Ser Ala Gly 
    1280                 1285                 1290             


Ser Leu  Leu Asn Gly Thr Leu  Ile Tyr Gln Ser Lys  Thr Gly Leu 
    1295                 1300                 1305             


Tyr Glu  Thr Arg Ile Asp Leu  Gly Lys Leu 
    1310                 1315             


<210>  819
<211>  1319
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  819

Met Phe Ser Gln Arg Asn Asn Pro Tyr Cys Glu Leu Leu Phe Lys Ile 
1               5                   10                  15      


Ile Val Thr Asn Ile Glu Glu Lys Asn Leu Glu Val Ile Met Gly Ile 
            20                  25                  30          


Arg Asn Ser Asp Ile Phe Phe Ile Ser Asn Phe Met Trp Tyr Gln Lys 
        35                  40                  45              


Leu Ile Met Met Ile Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn 
    50                  55                  60                  


Ser Ile Gly Trp Ala Leu Ile Glu Val Asp Glu Asn Asn Ile Pro Ile 
65                  70                  75                  80  


Arg Ile Ile Ala Met Gly Ser Arg Ile Ile Pro Leu Ser Thr Ser Asp 
                85                  90                  95      


Lys Glu Glu Phe Gln Arg Gly Leu Ser Ile Thr Lys Asn Gln Asn Arg 
            100                 105                 110         


Thr Val Ser Arg Thr Gln Arg Lys Gly Tyr Asp Arg Lys Gln Leu Arg 
        115                 120                 125             


Arg Ser Asp Leu Lys Lys Ile Leu Lys Lys Tyr Asn Ile Phe Pro Ser 
    130                 135                 140                 


Glu Glu Leu Leu Lys Leu Pro Met Leu Asp Leu Trp Lys Leu Arg Ser 
145                 150                 155                 160 


Asp Ser Ala Asn Pro Asn Glu Asp Ile Ser Ala Glu Gln Leu Gly Arg 
                165                 170                 175     


Ile Leu Tyr Met Leu Asn Gln Lys Arg Gly Tyr Lys Ser Ala Arg Ser 
            180                 185                 190         


Glu Ala Asn Ser Asp Lys Lys Asp Thr Asp Tyr Val Gln Ala Val Lys 
        195                 200                 205             


Gly Arg Tyr Ala Gln Leu Lys Asp Arg Arg Gln Thr Ile Gly Gln Tyr 
    210                 215                 220                 


Phe Tyr Glu Glu Leu Ser Thr Ala Phe Ser Asp Lys Asn Asn Gln Gln 
225                 230                 235                 240 


Tyr Phe Arg Val Lys Glu Glu Val Tyr Pro Arg Glu Ala Tyr Ile Glu 
                245                 250                 255     


Glu Phe Asp Thr Ile Ile Asn Ala Gln Lys Asp Lys His Gly Phe Leu 
            260                 265                 270         


Thr Asp Lys Val Ile His Gln Leu Arg Asn Glu Ile Ile Tyr Phe Gln 
        275                 280                 285             


Arg Lys Leu Lys Ser Gln Lys Gly Leu Val Ser Ile Cys Glu Leu Glu 
    290                 295                 300                 


Gly Phe Glu Lys Thr Val Tyr Asp Lys Glu Lys Asn Lys Asn Lys Val 
305                 310                 315                 320 


Val Phe Val Gly Pro Lys Val Ala Pro Lys Thr Ser Pro Leu Phe Gln 
                325                 330                 335     


Leu Cys Arg Ile Trp Glu Val Val Asn Thr Ile Ser Leu Lys Ile Lys 
            340                 345                 350         


Asn Pro Glu Gly Ser Lys Tyr Lys Trp Gly Glu Lys Val Pro Asn Leu 
        355                 360                 365             


Lys Gln Lys Glu Ile Leu Ala Asp Tyr Leu Phe Lys Asn Glu Asn Leu 
    370                 375                 380                 


Ser Phe Ser Thr Leu Leu Glu Ile Leu Glu Leu Lys Lys Glu Asp Val 
385                 390                 395                 400 


Tyr Val Asn Lys Gln Ile Leu Lys Gly Ile Lys Gly Asn Glu Ala Tyr 
                405                 410                 415     


Ala Ala Leu His Asn Val Leu Gly Asp His Glu Leu Leu Asn Phe Asp 
            420                 425                 430         


Val Ser Ile Ile Thr Ser Asp His Thr Ala Ile Leu Val Asp Lys Lys 
        435                 440                 445             


Thr Gly Glu Ile Leu Glu Glu Arg Ala Gly Leu Glu Leu Asp Ala Ser 
    450                 455                 460                 


Leu Glu Arg Glu Pro Leu Tyr Gln Phe Trp His Thr Ile Tyr Ser Leu 
465                 470                 475                 480 


Lys Asp Leu Asp Glu Cys Lys Asn Ala Leu Ile Lys Arg Phe Gly Leu 
                485                 490                 495     


Asp Glu Asp Ile Ala Glu Lys Leu Ser His Ile Asp Phe Asn Lys Gln 
            500                 505                 510         


Ala Phe Gly Ser Lys Ser Asn Lys Ser Met Arg Lys Ile Leu Pro Phe 
        515                 520                 525             


Leu Met Glu Gly Tyr Asn Tyr Ser Glu Ser Cys Ser Leu Ala Gly Tyr 
    530                 535                 540                 


Asn His Ser Asn Ser Leu Thr Lys Asp Glu Arg Glu Ser Gln Ile Thr 
545                 550                 555                 560 


Leu Glu Arg Leu Glu Leu Leu Pro Lys Asn Asn Leu Arg Gln Pro Ile 
                565                 570                 575     


Val Glu Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Thr Ile Leu 
            580                 585                 590         


Glu Lys Tyr Gly Lys Pro Ser Glu Ile Arg Val Glu Leu Ala Arg Glu 
        595                 600                 605             


Leu Lys Gln Ser Lys Asp Glu Arg Asn Asp Thr Asp Leu Gln Asn Ser 
    610                 615                 620                 


Arg Asn Lys Lys Leu Asn Glu Glu Ile Gly Lys Arg Leu Ala Glu Leu 
625                 630                 635                 640 


Gly Leu Pro Val Thr Lys Arg Tyr Ile Gln Lys Tyr Lys Phe Ile Phe 
                645                 650                 655     


Pro Ser Leu Ser Lys Asn Leu Lys Asp Ala His Val Asn Asn Gln Cys 
            660                 665                 670         


Ile Tyr Cys Gly Glu Thr Phe Asn Leu Thr Glu Ala Leu Asn Gly Asp 
        675                 680                 685             


Ala Tyr Asp Val Asp His Ile Val Pro Lys Ala Leu Leu Phe Asp Asp 
    690                 695                 700                 


Ser Gln Thr Asn Lys Val Leu Val His Arg Lys Cys Asn Ser Thr Lys 
705                 710                 715                 720 


Thr Asn Gln Thr Ala Tyr Asp Tyr Ile Thr Ala Lys Gly Glu Glu Glu 
                725                 730                 735     


Val Arg Ile Tyr Ser Glu Arg Val Asp Asp Trp Phe Lys Lys Gly Ile 
            740                 745                 750         


Ile Ser Tyr Ser Lys Met Gln Arg Leu Lys Val Ser His Lys Glu Tyr 
        755                 760                 765             


Leu Glu Arg Lys Lys Leu Gly Lys Glu Thr Glu Thr Asp Lys Lys Leu 
    770                 775                 780                 


Trp Glu Asn Phe Ile Asp Arg Gln Leu Arg Asp Thr Gln Tyr Ile Ser 
785                 790                 795                 800 


Arg Lys Ser Arg Glu Ile Leu Gln Lys Val Cys Asn Asn Val Thr Thr 
                805                 810                 815     


Thr Glu Gly Gly Val Thr Ala Lys Leu Arg Asn Leu Trp Gly Trp Asp 
            820                 825                 830         


Asp Ile Leu Met Asn Leu Gln Met Pro Lys Tyr Lys Glu Leu Gly Gln 
        835                 840                 845             


Thr Ile Thr Lys Glu Trp Thr Ser Glu His Gly Lys Arg Lys His Gln 
    850                 855                 860                 


Lys Glu Glu Ile Glu Asn Trp Thr Lys Arg Asp Asp His Arg His His 
865                 870                 875                 880 


Ala Ile Asp Ala Leu Val Val Ala Cys Thr Lys Gln Gly Phe Ile Gln 
                885                 890                 895     


Arg Ile Asn Thr Leu Asn Ser Ser Glu Thr Lys Asp Leu Met Ile Lys 
            900                 905                 910         


Glu Val Glu Glu Ala Lys Ile Glu Phe Asn Glu Lys Thr Thr Leu Leu 
        915                 920                 925             


Glu Lys Tyr Leu Lys Thr Gln Lys Pro Phe Thr Thr Arg Glu Val Met 
    930                 935                 940                 


Lys Glu Ala Asp Lys Ile Leu Ile Ser Phe Lys Ala Gly Lys Lys Val 
945                 950                 955                 960 


Ala Thr Ile Thr Lys Phe Lys Ala Thr Gly Lys Asn Lys Glu Thr Gly 
                965                 970                 975     


Val Ile Val Pro Arg Gly Ala Leu His Glu Gln Ser Val Tyr Gly Lys 
            980                 985                 990         


Ile Lys Val Ile Glu Lys Asp Lys  Pro Leu Lys Tyr Leu  Phe Glu Asn 
        995                 1000                 1005             


Pro Asp  Lys Ile Val Asn Ser  Arg Ile Arg Asp Leu  Val Glu Thr 
    1010                 1015                 1020             


Arg Leu  Ser Glu Asn Glu Asn  Asn Ser Lys Lys Ala  Leu Ser Thr 
    1025                 1030                 1035             


Val Lys  Lys Asn Pro Ile Phe  Phe Asn Glu Glu Lys  Asn Glu Ile 
    1040                 1045                 1050             


Leu Glu  Lys Ala Ser Cys Tyr  Asn Asp Ala Thr Val  Leu Lys Tyr 
    1055                 1060                 1065             


Lys Leu  Gln Asn Leu Lys Ala  Asn Gln Val Asp Asp  Ile Val Asp 
    1070                 1075                 1080             


Glu Arg  Val Lys Phe Leu Val  Lys Glu Arg Leu Thr  Gln Phe Gly 
    1085                 1090                 1095             


Ser Lys  Glu Lys Glu Ala Phe  Lys Asp Ile Leu Trp  Phe Asn Gln 
    1100                 1105                 1110             


Glu Lys  Gln Ile Pro Ile Ile  Thr Val Arg Leu Phe  Ala Arg Pro 
    1115                 1120                 1125             


Asp Ala  Asn Asn Leu Gln Ile  Ile Lys Lys Asp Lys  Asn Gly Lys 
    1130                 1135                 1140             


Asp Ile  Gly Phe Val Leu Thr  Gly Asn Asn His His  Ile Ala Ile 
    1145                 1150                 1155             


Tyr Glu  Asp Lys Asp Ser Lys  Leu Val Gln His Ser  Cys Ala Phe 
    1160                 1165                 1170             


Trp His  Ala Val Glu Arg Lys  Lys Tyr Asn Ile Pro  Ala Val Ile 
    1175                 1180                 1185             


Lys Asn  Ser Ala Asp Val Trp  Ser Leu Leu Leu Asp  Lys Glu Leu 
    1190                 1195                 1200             


Pro Val  Leu Phe Leu Asp Lys  Leu Pro Ala Asp Glu  Leu Asn Leu 
    1205                 1210                 1215             


Lys Phe  Ser Met Gln Gln Asn  Glu Met Phe Ile Leu  Gly Leu Ser 
    1220                 1225                 1230             


Gln Glu  Gly Phe Asp Glu Ala  Ile Lys Asn Asn Asp  Lys Ser Leu 
    1235                 1240                 1245             


Leu Ser  Lys His Leu Tyr Leu  Val Trp Ser Ile Ser  Asn Asn Asp 
    1250                 1255                 1260             


Tyr Tyr  Phe Arg His His Leu  Glu Thr Lys Asn Thr  Glu Leu Lys 
    1265                 1270                 1275             


Asn Ile  Pro Thr Ala Lys Asp  Ser Lys Arg Tyr Phe  Arg Phe Lys 
    1280                 1285                 1290             


Ser Val  Gly Ala Leu Val Gly  Ile Asn Pro Ile Lys  Val Arg Leu 
    1295                 1300                 1305             


Asn His  Leu Gly Glu Ile Thr  Lys Ile Gly Glu 
    1310                 1315                 


<210>  820
<211>  1319
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  820

Met Gly Tyr Asn Ile Gly Leu Asp Ile Gly Thr Gly Ser Val Gly Trp 
1               5                   10                  15      


Ala Ala Leu Thr Asp Glu Gly Lys Leu Ala Arg Ala Lys Gly Lys Asn 
            20                  25                  30          


Leu Ile Gly Val Arg Leu Phe Asp Ser Ala Gln Ser Ala Ala Gln Arg 
        35                  40                  45              


Arg Ser Tyr Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg Lys Trp Arg 
    50                  55                  60                  


Leu Arg Leu Leu Glu Asn Ile Phe Ser Asp Glu Met Gly Met Ile Asp 
65                  70                  75                  80  


Glu Asn Phe Phe Ala Arg Leu Lys Tyr Ser Tyr Val His Pro Lys Asp 
                85                  90                  95      


Glu Val Asn Asn Ala His Tyr Tyr Gly Gly Tyr Leu Phe Pro Thr Gln 
            100                 105                 110         


Gln Glu Thr His Asp Phe His Glu Lys Phe Gln Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Leu Lys Leu Met Ile Glu Asp Cys Lys Phe Asp Leu Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Met His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Ser Gln Ser Lys Met Thr Ile Gly Asp Ser Tyr Asn Pro Arg Asp Phe 
                165                 170                 175     


Gln Gln Ala Ile Gln Asn Tyr Ala Glu Ala Lys Gly Leu Ile Trp Ser 
            180                 185                 190         


Leu Asn Asp Ala Gln Glu Met Thr Asp Val Leu Val Gly Gln Ala Gly 
        195                 200                 205             


Phe Gly Leu Ser Lys Lys Ala Lys Ala Glu Arg Leu Leu Ser Ala Phe 
    210                 215                 220                 


Ser Phe Asp Thr Lys Glu Asp Lys Lys Ala Ile Gln Ala Ile Leu Ala 
225                 230                 235                 240 


Gly Ile Val Gly Asn Thr Thr Asp Phe Thr Lys Ile Phe Asn Arg Glu 
                245                 250                 255     


Arg Ser Gly Asp Glu Leu Lys Lys Trp Lys Leu Lys Leu Asp Ser Glu 
            260                 265                 270         


Ala Phe Asp Glu Gln Ser Gln Ala Ile Val Asp Glu Leu Asp Asp Asp 
        275                 280                 285             


Glu Met Glu Leu Phe Asn Ala Ile Arg Gln Ala Phe Asp Gly Phe Thr 
    290                 295                 300                 


Leu Met Asp Leu Leu Gly Asp Gln Thr Ser Ile Ser Ala Ala Met Val 
305                 310                 315                 320 


Lys Arg Tyr Gln Gln His His Asp Asp Leu Lys Met Val Lys Glu Ile 
                325                 330                 335     


Ala Lys Lys Gln Gly Leu Ser His Gln Asp Phe Ser Lys Ile Tyr Thr 
            340                 345                 350         


Ala Phe Leu Lys Asp Asp Thr Asp Lys Gly Met Lys Ala Leu Leu Asp 
        355                 360                 365             


Lys Ala Asp Leu Ala Asp Asp Val Leu Val Glu Ile Gln Gln Arg Ile 
    370                 375                 380                 


Glu Ser His Asp Phe Leu Pro Lys Gln Arg Thr Lys Ala Asn Ser Val 
385                 390                 395                 400 


Ile Pro Tyr Gln Leu His Leu Ala Glu Leu Glu Lys Ile Ile Glu Asn 
                405                 410                 415     


Gln Gly Lys Tyr Tyr Pro Phe Leu Leu Asp Thr Phe Thr Asn Lys Ala 
            420                 425                 430         


Gly Glu Thr Ile Asn Lys Leu Val Glu Leu Val Lys Phe Arg Val Pro 
        435                 440                 445             


Tyr Tyr Val Gly Pro Met Val Thr Ala Ala Asp Val Glu Lys Ala Gly 
    450                 455                 460                 


Gly Asp Ala Thr Asn His Trp Val Lys Arg Asn Glu Gly Tyr Glu Lys 
465                 470                 475                 480 


Ser Pro Val Thr Pro Trp Asn Phe Asp Gln Val Phe Asn Arg Asp Gln 
                485                 490                 495     


Ala Ala Gln Asp Phe Ile Asp Arg Leu Thr Gly Thr Asp Thr Tyr Leu 
            500                 505                 510         


Ile Gly Glu Pro Thr Leu Leu Lys Asn Ser Leu Lys Tyr Gln Leu Phe 
        515                 520                 525             


Thr Val Leu Asn Glu Leu Asn Asn Val Lys Ile Asn Gly His Lys Ile 
    530                 535                 540                 


Asp Glu Lys Thr Lys His Val Leu Ile Gln Asp Leu Phe Lys Ser Lys 
545                 550                 555                 560 


Lys Thr Val Ser Glu Lys Ala Ile Lys Asp Tyr Tyr Leu Ser Gln Gly 
                565                 570                 575     


Met Gly Glu Ile Gln Ile Val Gly Leu Ala Asp Lys Thr Lys Phe Asn 
            580                 585                 590         


Ser Asn Leu Ser Ser Tyr Ile Asp Leu Ser Lys Thr Phe Asp Ala Glu 
        595                 600                 605             


Phe Met Glu Asn Pro Ala Asn Gln Glu Leu Leu Glu Asn Ile Ile Gln 
    610                 615                 620                 


Ile Gln Thr Val Phe Glu Asp Val Lys Ile Ala Glu Arg Glu Leu Gln 
625                 630                 635                 640 


Lys Leu Ala Leu Pro Asp Glu Gln Val Gln Gln Leu Ala Lys Thr His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Asn Leu Ser Asp Lys Leu Leu Ser Thr Pro Ile 
            660                 665                 670         


Ile Gln Glu Gly Ser Gln Lys Val Ser Ile Leu Asn Lys Leu Gln Thr 
        675                 680                 685             


Thr Ser Lys Asn Phe Met Ser Ile Ile Thr Asp Asn Lys Phe Gly Val 
    690                 695                 700                 


Gln Gln Trp Ile Gln Glu Gln Asn Thr Ala Glu Thr Ala Asp Ser Ile 
705                 710                 715                 720 


Gln Asp Arg Ile Asp Glu Leu Thr Thr Ala Pro Ala Asn Lys Arg Gly 
                725                 730                 735     


Ile Lys Gln Ala Phe Asn Val Leu Phe Asp Ile Gln Lys Ala Met Gly 
            740                 745                 750         


Glu Glu Pro Asn Arg Val Tyr Leu Glu Phe Ala Lys Glu Thr Gln Asn 
        755                 760                 765             


Ser Val Arg Thr Asn Ser Arg Tyr Asn Arg Leu Lys Asp Leu Tyr Lys 
    770                 775                 780                 


Ser Lys Thr Leu Ser Asp Asp Val Lys Ala Leu Lys Glu Glu Leu Glu 
785                 790                 795                 800 


Ser Gln Lys Ser Ser Leu Gln Ser Glu Arg Ile Gly Asp Arg Leu Tyr 
                805                 810                 815     


Leu Tyr Phe Leu Gln Gln Gly Lys Asp Met Tyr Thr Gly Gln Pro Ile 
            820                 825                 830         


Asn Ile Asp Lys Leu Ser Thr Asp Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Tyr Thr Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Arg Pro Glu Asn Ala Arg Lys Ser Asp Ser Ala Thr Tyr Thr Thr Glu 
865                 870                 875                 880 


Val Gln Gln Ser Ala Gly Gly Leu Trp Lys Ser Leu Lys Asn Ala Gly 
                885                 890                 895     


Phe Ile Ser Gln Lys Lys Tyr Asp Arg Leu Thr Lys Gly Gly Asp Tyr 
            900                 905                 910         


Ser Lys Gly Gln Lys Thr Gly Phe Ile Ala Arg Gln Leu Val Glu Thr 
        915                 920                 925             


Arg Gln Ile Ile Lys Asn Val Ala Ser Leu Ile Glu Ser Glu Phe Ser 
    930                 935                 940                 


Gln Thr Lys Ala Val Ala Ile Arg Ser Glu Ile Thr Ala Asp Met Arg 
945                 950                 955                 960 


Arg Leu Val Ala Ile Lys Lys His Arg Glu Ile Asn Ser Phe His His 
                965                 970                 975     


Ala Phe Asp Ala Leu Leu Ile Thr Ala Ala Gly Gln Tyr Met Gln Ala 
            980                 985                 990         


Arg Tyr Pro Asp Arg Asp Gly Ala  Asn Val Tyr Asn Glu  Phe Asp Tyr 
        995                 1000                 1005             


Tyr Thr  Asn Thr Tyr Leu Lys  Glu Leu Arg Gln Ser  Ser Ser Ser 
    1010                 1015                 1020             


Ser Gln  Val Arg Arg Leu Lys  Pro Phe Gly Phe Val  Val Gly Thr 
    1025                 1030                 1035             


Met Ala  Lys Gly Asn Glu Asn  Trp Ser Glu Asp Asp  Thr Gln Tyr 
    1040                 1045                 1050             


Leu Arg  His Val Met Asn Phe  Lys Asn Ile Leu Thr  Thr Arg Arg 
    1055                 1060                 1065             


Asn Asp  Lys Asp Asn Gly Ala  Leu Asn Lys Glu Thr  Ile Tyr Ala 
    1070                 1075                 1080             


Val Asp  Pro Lys Ala Lys Leu  Ile Gly Thr Asn Lys  Lys Arg Gln 
    1085                 1090                 1095             


Asp Val  Ser Leu Tyr Gly Gly  Tyr Ile Tyr Pro Tyr  Ser Ala Tyr 
    1100                 1105                 1110             


Met Thr  Leu Val Arg Ala Asn  Gly Lys Asn Leu Leu  Val Lys Val 
    1115                 1120                 1125             


Thr Ile  Ser Ala Ala Glu Lys  Ile Lys Ser Gly Gln  Ile Glu Leu 
    1130                 1135                 1140             


Ser Glu  Tyr Val Gln Gln Arg  Pro Glu Val Lys Lys  Phe Glu Lys 
    1145                 1150                 1155             


Ile Leu  Ile Asn Lys Leu Ala  Ile Gly Gln Leu Val  Asn Asn Asp 
    1160                 1165                 1170             


Gly Asn  Leu Ile Tyr Leu Thr  Ser Tyr Glu Phe Tyr  His Asn Ala 
    1175                 1180                 1185             


Lys Gln  Leu Trp Leu Pro Thr  Glu Glu Ala Asp Leu  Ile Ser Gln 
    1190                 1195                 1200             


Leu Asn  Lys Asp Ser Ser Asp  Glu Asp Leu Ile Lys  Gly Phe Asp 
    1205                 1210                 1215             


Ile Leu  Thr Ser Pro Ala Ile  Leu Lys Arg Phe Pro  Phe Tyr Glu 
    1220                 1225                 1230             


Leu Asp  Leu Lys Lys Leu Val  Asn Ile Arg Asp Lys  Phe Ile Ala 
    1235                 1240                 1245             


Val Glu  Asn Lys Phe Asp Ile  Leu Met Val Ile Leu  Lys Ala Leu 
    1250                 1255                 1260             


Gln Leu  Asp Ala Ala Gln Gln  Lys Pro Val Lys Met  Ile Asp Lys 
    1265                 1270                 1275             


Lys Ser  Ala Asp Trp Lys Asp  Tyr Arg Gln Arg Gly  Gly Ile Lys 
    1280                 1285                 1290             


Leu Ser  Asp Thr Ser Glu Ile  Ile Tyr Gln Ser Thr  Thr Gly Ile 
    1295                 1300                 1305             


Phe Glu  Lys Arg Val Lys Ile  Ser Asn Leu Leu 
    1310                 1315                 


<210>  821
<211>  1320
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  821

Met Thr Glu Gln Gln Arg Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Ser Ser Ile Gly Trp Ala Val Thr Asp Gln Asn Asp Lys Leu Leu His 
            20                  25                  30          


Ile Arg Gly His Asn Gly Ile Gly Val Arg Leu Phe Lys Glu Gly Glu 
        35                  40                  45              


Ser Ala Ala Glu Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Arg Arg Trp Arg Leu Arg Phe Leu Asp Arg Ile Phe Asp Ala Glu 
65                  70                  75                  80  


Leu Ala Lys Val Asp Pro Asn Phe Leu Ala Arg Leu Lys Gln Ser Asn 
                85                  90                  95      


Ile Ser Pro Leu Asp Pro Asn Lys Gln Met Phe Gly Asn Ile Leu Phe 
            100                 105                 110         


Asp Asp Ala Gln Phe Asp Asp Gln Lys Phe His Gln Gln Tyr Pro Thr 
        115                 120                 125             


Ile Tyr His Leu Arg Ser Ala Leu Ala Gln Pro Asp Ala Pro Gln Ala 
    130                 135                 140                 


Asp Val Arg Leu Ile Tyr Leu Ala Leu His His Met Ile Lys Phe Arg 
145                 150                 155                 160 


Gly His Phe Leu Asn Gly Ala Ala Val Glu Lys Phe His Gly Gly Gln 
                165                 170                 175     


Leu His Leu Glu Ala Glu Phe Gln Glu Leu Asn Gln Ile Phe Ser Asn 
            180                 185                 190         


Gln Gln Arg Asp His Val Gln Leu Gln Thr Ser Ser Leu Asp Glu Val 
        195                 200                 205             


Ala Thr Ile Leu Lys Asp Asn Gln Arg Thr Pro Ser Asp Arg Gln Arg 
    210                 215                 220                 


Glu Leu Ser Ala Arg Leu Phe Val Pro Val Asp Lys Thr Ile Asp Lys 
225                 230                 235                 240 


Glu Asn Lys Lys Leu Ala Thr Glu Leu Leu Lys Ala Val Leu Gly Leu 
                245                 250                 255     


Lys Ala Lys Phe Asp Val Ile Leu Gly Val Glu Ala Thr Asp Pro Gln 
            260                 265                 270         


Ala Phe Lys Leu Gln Phe Ser Ser Asp Asp Phe Asp Asp Lys Ile Ala 
        275                 280                 285             


Glu Leu Ala Ser Glu Leu Thr Asp Glu Ala Asn Glu Ile Leu Thr Val 
    290                 295                 300                 


Leu Gln Arg Leu Tyr Phe Ser Leu Asn Leu Ala Asp Ile Leu Thr Asn 
305                 310                 315                 320 


Arg Gln Thr Lys Gln Val Ala Asp Ser Ile Ser Thr Ala Met Ile Asn 
                325                 330                 335     


Arg Tyr Asp Asp His Lys His His Leu Lys Met Leu Lys Glu Leu Glu 
            340                 345                 350         


Thr Gln Val Thr Gln Glu Gln Arg Asp Gly Leu Lys Lys Ala Tyr Asp 
        355                 360                 365             


Glu Tyr Ile Asp Gly Asp Lys Pro Thr Ile Asp Thr Phe Tyr Thr Asn 
    370                 375                 380                 


Val Lys Lys Asn Leu Asp Asp Ser Glu Leu Ala Asn Gln Ile Lys Ser 
385                 390                 395                 400 


Leu Ile Asp Leu Gln Ala Phe Met Pro Lys Gln Arg Ser Lys Glu Asn 
                405                 410                 415     


Gly Ser Ile Pro His Gln Leu His Gln Arg Glu Leu Asp Gln Leu Ile 
            420                 425                 430         


Glu Val Gln Gly Lys Tyr Tyr Pro Trp Leu Leu Ala Ala Lys Asp Lys 
        435                 440                 445             


Leu Asp Ala Leu Ile Ala Phe Arg Val Pro Tyr Tyr Val Gly Pro Met 
    450                 455                 460                 


Ile Asp Pro Asn Glu Ser Asp Asp Pro Gln Asn Thr Lys Phe Ala Trp 
465                 470                 475                 480 


Met Val Arg Lys Glu Ala Gly Glu Ile Thr Pro Trp Asn Phe Glu Glu 
                485                 490                 495     


Lys Val Asp Lys Thr Gly Ser Ala Thr Asn Phe Ile Lys Arg Met Thr 
            500                 505                 510         


Thr Thr Asp Thr Tyr Leu Leu Gly Glu Pro Val Leu Pro Lys Asn Ser 
        515                 520                 525             


Leu Leu Tyr Gln Gln Phe Thr Val Leu Asn Glu Leu Asn Lys Leu Lys 
    530                 535                 540                 


Val Asp Gly Lys Ala Leu Thr Val Asp Gln Lys Lys Tyr Leu Tyr Glu 
545                 550                 555                 560 


His Val Cys Lys Lys Gln Arg Arg Val Thr Leu Lys Lys Leu Ser Asp 
                565                 570                 575     


Cys Leu Met Ala Arg Gly Asp Phe Pro Met Gly Ala Ile Phe Thr Gly 
            580                 585                 590         


Thr Thr Asp Gln Lys Asn Met Asn Asn Thr Leu Lys Thr Tyr Gln Asp 
        595                 600                 605             


Phe Gln Glu Ile Phe Gly Asp Glu Ile Asp Asn Pro Asp Arg Gln Val 
    610                 615                 620                 


Asp Phe Glu Lys Met Ile Glu Trp Ser Thr Ile Phe Glu Asp Arg Glu 
625                 630                 635                 640 


Ile Phe Lys Leu Lys Leu Ala Glu Leu Asn Trp Leu Thr Ser Glu Gln 
                645                 650                 655     


Leu Glu Arg Val Ser Lys Lys Arg Tyr Gln Gly Trp Gly Arg Leu Ser 
            660                 665                 670         


Gln Lys Leu Leu Thr His Ile Val Asn Glu Asn Gly Glu Arg Ile Ile 
        675                 680                 685             


Asp Gln Leu Trp Asn Thr Asn Gln Asn Phe Met Glu Ile Met Gly Gln 
    690                 695                 700                 


Asp Gly Val Lys Gln Ala Ile Gln Glu His Asn Gly Ala Asn Val Ala 
705                 710                 715                 720 


Gln Thr Gly Ile Asn Glu Thr Ile Asp Glu Leu Tyr Thr Ser Pro Gln 
                725                 730                 735     


Asn Lys Lys Ala Ile Arg Gln Ile Leu Leu Val Val Asp Asp Ile Lys 
            740                 745                 750         


Lys Ala Val Gly Tyr Ala Pro Ala Asn Ile Met Met Glu Phe Ala Arg 
        755                 760                 765             


Glu Asp Arg Asp Asp His Arg Leu Ile Ala Ser Arg Ala Arg Gln Leu 
    770                 775                 780                 


Glu Lys Thr Tyr Gln Glu Ile Ser Lys Asn Trp Phe Asp Asn Glu Ser 
785                 790                 795                 800 


Val Thr Gln Glu Leu Lys Asp Gln Ile Lys Asn Lys Thr Lys Phe Thr 
                805                 810                 815     


Asp Arg Leu Tyr Leu Tyr Phe Ile Gln Gly Gly Ile Asp Leu Tyr Thr 
            820                 825                 830         


Gly His Pro Leu Asn Ile Asp Gln Leu Ser Ser Tyr Asp Ile Asp His 
        835                 840                 845             


Ile Leu Pro Gln Ser Phe Leu Leu Asp Asn Ser Leu Asn Asn Arg Val 
    850                 855                 860                 


Leu Thr Ser Gln Ser Glu Asn Arg Val His Lys Glu Asp Arg Leu Pro 
865                 870                 875                 880 


Gly Glu Thr Val Ala Asn Lys Met Arg Pro Thr Trp Lys Met Met Arg 
                885                 890                 895     


Glu Gln Gly Leu Met Thr Gln Arg Lys Tyr Asn Asn Leu Thr Leu Lys 
            900                 905                 910         


Pro Asp Gln Ile Ser Lys Phe Asn Asn Arg Gly Gln Phe Ile Asn Arg 
        915                 920                 925             


Gln Leu Val Glu Thr Arg Gln Val Ile Lys Leu Ala Ala Asp Ile Met 
    930                 935                 940                 


Asn Gln Lys Tyr Gln Ala Asp Gln Thr Lys Val Val Thr Val Lys Ala 
945                 950                 955                 960 


Asn Leu Thr His His Met Arg Glu Lys Phe Asn Phe Tyr Lys Ser Arg 
                965                 970                 975     


Asn Val Asn Asp Tyr His His Ala Phe Asp Ala Tyr Leu Thr Val Phe 
            980                 985                 990         


Val Gly Asn Trp Leu Leu Lys Glu  Tyr Pro Lys Leu Gln  Pro Tyr Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Phe Ala Lys  Thr Gly Thr Asn Asn  Leu Lys Ser 
    1010                 1015                 1020             


Phe Asn  Phe Leu Tyr Arg Phe  Glu Arg Asp His Lys  Leu Asn Asp 
    1025                 1030                 1035             


Lys Gly  Ala Ile Asp Thr Asp  Lys Asp Gln Gln Leu  Gly Tyr Met 
    1040                 1045                 1050             


Lys Arg  Ile Tyr Arg Phe Lys  Lys Met Leu Val Thr  Lys Gln Leu 
    1055                 1060                 1065             


Glu Thr  Asn Glu Gly Ala Leu  Tyr Lys Gln Thr Ile  Tyr Lys Ala 
    1070                 1075                 1080             


Pro Ala  Asn Glu Lys Asn Thr  Lys Lys Asn Arg Ser  Leu Ile Asn 
    1085                 1090                 1095             


Thr Lys  Lys Asn Arg Pro Ser  Asp Ile Tyr Gly Gly  Tyr Ser Gln 
    1100                 1105                 1110             


Ser Val  Thr Ala Tyr Met Ser  Leu Val Phe Phe Lys  Asn Thr Phe 
    1115                 1120                 1125             


Lys Leu  Ile Lys Met Pro Leu  Val Ala Ala Asn Ala  Ile Lys Asn 
    1130                 1135                 1140             


Ser Leu  Ser Ser Tyr His Asp  Thr Ile Leu Lys Ile  Leu Gly Ser 
    1145                 1150                 1155             


Asn Glu  Phe Lys Val Leu Ile  Pro Lys Val Lys Met  Asn Gln Leu 
    1160                 1165                 1170             


Ile Lys  Glu Gly Asp Ser Leu  Tyr Thr Val Val Gly  Val Tyr Lys 
    1175                 1180                 1185             


His Asn  Ala Gln Gln Leu Ile  Leu Ser Asp Asn Ser  Met Gly Ile 
    1190                 1195                 1200             


Ile Thr  Lys Pro Ile Arg Glu  Ile Cys Asp Ser Asp  Leu Asp Ser 
    1205                 1210                 1215             


Val Phe  Asn Glu Leu Val Asp  Asn Ile Asp Lys Tyr  Leu Asn Leu 
    1220                 1225                 1230             


Tyr Lys  Ile Glu Asp Lys Leu  Thr Ile Lys Glu Lys  Phe Tyr Ala 
    1235                 1240                 1245             


Leu Lys  Asn Lys Glu Ser Lys  Phe Lys Cys Ile Ser  Asp Ile Leu 
    1250                 1255                 1260             


Val Gly  Thr His Cys Asn Ala  Gly Arg Ser Asn Leu  Lys Ile Ile 
    1265                 1270                 1275             


Gly Leu  Ser Ser Tyr Trp Gly  Met Phe Ser Gly Asn  Asn Asp Gly 
    1280                 1285                 1290             


Leu Lys  Ile Asn Glu Asn Ser  Gln Leu Ile Tyr Gln  Ser Pro Thr 
    1295                 1300                 1305             


Gly Leu  Phe Glu His Arg Val  Lys Val Ser Asp Leu  
    1310                 1315                 1320 


<210>  822
<211>  1323
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  822

Met Lys Asp Phe Tyr Ile Gly Leu Asp Met Gly Thr Asp Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Ala Asp Glu Asp Tyr Asn Leu Cys Lys Phe Lys Gly Lys 
            20                  25                  30          


Ala Met Trp Gly Val Arg Leu Phe Asp Glu Ser Ala Thr Ala Ala Glu 
        35                  40                  45              


Arg Arg Gly Phe Arg Thr Ala Arg Arg Arg Ala Gln Arg Lys Arg Gln 
    50                  55                  60                  


Arg Ile Leu Trp Leu Gln Met Leu Phe Asn Glu Glu Ile Ser Lys Lys 
65                  70                  75                  80  


Asp Ile Ala Phe Phe Gln Arg Leu Asn Glu Ser Lys Leu Tyr Leu Glu 
                85                  90                  95      


Asp Lys Ser Thr Asn Val Pro Tyr Ala Val Phe Ala Asp Glu Ser Phe 
            100                 105                 110         


Thr Asp Val Asp Tyr His Lys Leu Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Lys Glu Leu Val Gln Ser Asp Glu Pro His Asp Val Arg Leu Val Tyr 
    130                 135                 140                 


Leu Ala Leu His His Leu Ile Lys Asn Arg Gly His Phe Leu Phe Glu 
145                 150                 155                 160 


Asn Val Gly Ser Asp Phe Glu Lys Glu Ser Ser Phe Glu Val Leu Phe 
                165                 170                 175     


Lys Asn Leu Gln Asn Tyr Ile Leu Gly Glu Tyr Glu Leu Ala Leu Glu 
            180                 185                 190         


Cys Ile Ser Pro Leu Gln Ile Ala Asp Ile Leu Lys Asp Lys Ser Lys 
        195                 200                 205             


Arg Lys Thr Thr Lys Ser Ser Glu Ile Cys Lys Leu Leu Gly Ile Ser 
    210                 215                 220                 


Lys Lys Gly Gln Pro Arg Leu Asn Ala Ile Val Leu Leu Met Cys Gly 
225                 230                 235                 240 


Gln Ser Val Lys Leu Ala Asp Leu Tyr Gly Asp Glu Asn Tyr Asn Lys 
                245                 250                 255     


Glu Glu Lys Ser Ser Ile Ser Phe Glu Lys Gly Tyr Glu Asp Asn Glu 
            260                 265                 270         


Glu Thr Tyr Arg Glu Leu Leu Leu Glu Arg Phe Glu Leu Ile Glu Lys 
        275                 280                 285             


Ile Lys Ala Leu Tyr Asp Trp Ala Ile Leu Ala Asp Ile Leu Lys Gly 
    290                 295                 300                 


Glu Thr Tyr Ile Ser Phe Ala Lys Val Lys Thr Tyr Asp Glu His Arg 
305                 310                 315                 320 


His Asp Leu Gln Gln Leu Lys Ala Phe Val Lys Glu Arg Cys Arg Ser 
                325                 330                 335     


Asp Tyr Asn Glu Ile Phe Arg Glu Thr Ser Asp Lys Leu Asn Asn Tyr 
            340                 345                 350         


Thr Ala Tyr Cys Gly Lys Phe Lys Glu Asn Gly Arg Asn Gly Val Ile 
        355                 360                 365             


Leu His Ser Ala Asn Gln Thr Asp Phe Cys Lys Tyr Leu Lys Ser Lys 
    370                 375                 380                 


Phe Asp Lys Leu Asp Lys Ser Gly Tyr Glu Asp Met Phe Glu Lys Ile 
385                 390                 395                 400 


Glu Asn Gly Ser Phe Met Pro Lys Ile Val Val Lys Asp Asn Gly Val 
                405                 410                 415     


Ile Pro Met Gln Val Asn Glu Ser Glu Val Arg Ala Ile Leu Lys Asn 
            420                 425                 430         


Ala Ser Gly Tyr Leu Asp Phe Leu Asn Asn Gln Asp Lys Asp Gly Ile 
        435                 440                 445             


Thr Val Ala Asp Lys Ile Leu Gln Ile Phe Glu Phe Arg Ile Pro Tyr 
    450                 455                 460                 


Tyr Val Gly Pro Leu Asn Val His Ser Asp Asn Ser Trp Leu Val Arg 
465                 470                 475                 480 


Lys Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asn Lys Ile Val Asp Val 
                485                 490                 495     


Asp Lys Ser Ala Glu Glu Phe Ile Asn Lys Leu Thr Ala Lys Cys Thr 
            500                 505                 510         


Tyr Leu Pro Asp Arg Asp Val Ile Pro Lys Asn Ser Ile Leu Tyr Ser 
        515                 520                 525             


Arg Tyr Met Val Leu Asn Glu Leu Asn Asn Leu Lys Leu Asp Gly Lys 
    530                 535                 540                 


Lys Pro Asp Val Ser Phe Lys Gln Ala Val Phe Asn Asp Leu Phe Met 
545                 550                 555                 560 


His Arg Arg Lys Val Thr Gln Lys Ala Leu Arg Glu Tyr Ile Lys Ala 
                565                 570                 575     


Gln Thr Gly Thr Glu Pro Glu Ile Thr Gly Ile Asp Gly Asp Phe Lys 
            580                 585                 590         


Ala Asn Met Arg Pro Ala Ile Glu Leu Ala Gly Tyr Asn Leu Ser Glu 
        595                 600                 605             


Pro Asp Lys Glu Glu Ile Ile Lys Ala Ile Thr Ile Phe Gly Asp Asp 
    610                 615                 620                 


Lys Lys Leu Leu Lys Arg Arg Ile Lys Asp Lys Tyr Ser Glu Met Leu 
625                 630                 635                 640 


Ser Glu Asp Glu Ile Lys Lys Ile Cys Lys Leu Lys Tyr Lys Asp Trp 
                645                 650                 655     


Gly Arg Leu Ser Arg Glu Phe Leu Thr Glu Ile Phe Asp Val Asp Thr 
            660                 665                 670         


Ala Thr Gly Glu Cys Lys Asp Asn Ile Ile Asn Thr Leu Trp Asn Thr 
        675                 680                 685             


Asn Asp Asn Leu Met Glu Leu Leu Gly Ala Lys Tyr Asn Phe Thr Gln 
    690                 695                 700                 


Ser Ile Glu Gln Ala Ile Ser Lys Ser Thr His Lys Ala Ser Ile Glu 
705                 710                 715                 720 


Lys Met Val Glu Asp Met Tyr Val Ser Pro Lys Val Lys Arg Thr Val 
                725                 730                 735     


Phe Gln Ser Leu Lys Ile Met Lys Glu Ile Val Lys Ile Lys Gly His 
            740                 745                 750         


Ala Pro Lys Lys Val Phe Val Glu Met Thr Arg His Asp Gly Thr Lys 
        755                 760                 765             


Gly Asp Asn Gly Arg Thr Lys Ser Arg Lys Lys Gln Leu Glu Glu Leu 
    770                 775                 780                 


Tyr Lys Lys Cys Lys Glu Asp Ser Thr Glu Leu Trp Asp Glu Leu Glu 
785                 790                 795                 800 


Lys Thr Asp Asp Asp Glu Phe Arg Gln Asp Lys Leu Phe Leu Tyr Tyr 
                805                 810                 815     


Thr Gln Met Gly Arg Cys Met Tyr Ser Gly Glu Arg Ile Glu Leu Arg 
            820                 825                 830         


Asp Leu Phe Asn Lys Asn Leu Tyr Asp Ile Asp His Ile Phe Pro Arg 
        835                 840                 845             


Ser Lys Val Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Lys Lys 
    850                 855                 860                 


Gln Ile Asn Ser His Lys Asp Asn Asp Tyr Pro Leu Asp Ser Thr Val 
865                 870                 875                 880 


Arg Glu Lys Met Arg Gly His Trp Gln Tyr Leu Leu Arg His Gly Phe 
                885                 890                 895     


Ile Ser Lys Lys Lys Tyr Asp Arg Leu Thr Arg Val Thr Pro Leu Thr 
            900                 905                 910         


Asp Asp Glu Leu Ala Asp Phe Ile Ala Arg Gln Leu Val Glu Thr Ser 
        915                 920                 925             


Gln Ser Thr Lys Ala Val Ala Thr Leu Leu Lys Thr Leu Tyr Pro Thr 
    930                 935                 940                 


Thr Glu Ile Val Tyr Val Lys Ala Glu Leu Val Ser Arg Phe Arg Thr 
945                 950                 955                 960 


Glu Tyr Lys Phe Ile Lys Cys Arg Glu Val Asn Asp Leu His His Ala 
                965                 970                 975     


Lys Asp Ala Tyr Leu Asn Ile Val Val Gly Asn Val Tyr Asn Val Arg 
            980                 985                 990         


Tyr Thr His Ser Lys Ala Ile Phe  Ile Lys Gly Leu Gln  Thr Lys Lys 
        995                 1000                 1005             


Tyr Ser  Leu Asn Arg Met Phe  Ala Phe Asp Thr Pro  Gly Ala Trp 
    1010                 1015                 1020             


Asn Ile  Glu Asn Glu Lys Ser  Leu Ser Ile Val Lys  Lys Thr Val 
    1025                 1030                 1035             


Met Lys  Asn Asn Ile Leu Tyr  Thr Arg Tyr Ser Tyr  Met Lys Lys 
    1040                 1045                 1050             


Gly Gly  Leu Phe Lys Ile Gln  Pro Leu Lys Lys Gly  Asn Gly Gln 
    1055                 1060                 1065             


Ala Pro  Leu Lys Lys Asp Leu  Pro Leu Ser Asp Ile  Gly Lys Tyr 
    1070                 1075                 1080             


Gly Gly  Tyr Asp Lys Pro Thr  Ala Ser Tyr Phe Ser  Tyr Ile Lys 
    1085                 1090                 1095             


Tyr Glu  Gly Lys Lys Gly Lys  Glu Cys Arg Gln Leu  Val Ala Ile 
    1100                 1105                 1110             


Asp Asn  Tyr Leu Lys Lys Tyr  Tyr Glu Glu Asn Pro  Ile Glu Tyr 
    1115                 1120                 1125             


Leu Thr  Asn Arg Leu Gly Leu  Lys Asn Pro Ala Val  Leu Ile Pro 
    1130                 1135                 1140             


Val Ile  Lys Tyr Asn Ser Cys  Ile Glu Ile Asp Gly  Phe Arg Met 
    1145                 1150                 1155             


Asn Ile  Ser Ser Lys Ser Asn  Glu Gly Lys Thr Ile  Val Tyr Lys 
    1160                 1165                 1170             


Pro Ala  Val Gln Leu Val Leu  Gly Ala Glu Lys Glu  Ala Tyr Val 
    1175                 1180                 1185             


Lys Lys  Ile Ser Lys Val Leu  Ser Lys Pro Glu Glu  Gln Lys Ile 
    1190                 1195                 1200             


Thr Ser  Tyr Asp Gly Val Ser  Ala Glu Glu Asn Ile  Glu Leu Phe 
    1205                 1210                 1215             


Asp Ala  Leu Thr Ala Lys Met  Thr Asn Thr Ile Phe  Lys Ile Lys 
    1220                 1225                 1230             


Leu Glu  Asp Ile Gly Asn Lys  Ile Lys Ser Gly Arg  Asp Lys Phe 
    1235                 1240                 1245             


Val Thr  Leu Asp Val Lys Lys  Gln Cys Tyr Val Ile  Asn Glu Ile 
    1250                 1255                 1260             


Leu Lys  Ile Met His Cys Asn  Val Val Thr Gly Asp  Leu Ser Leu 
    1265                 1270                 1275             


Ile Gly  Ala Ala Lys Lys Ala  Gly Ile Val Ser Thr  Asn Ser Ala 
    1280                 1285                 1290             


Leu Ser  Glu Ile Lys Lys Val  Ser Ser Ile Met Leu  Ile Asn Gln 
    1295                 1300                 1305             


Ser Val  Thr Gly Leu Phe Glu  Thr Lys Ile Asp Leu  Leu Asn Ile 
    1310                 1315                 1320             


<210>  823
<211>  1324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  823

Met Arg Ala Lys Gly Lys Thr Leu Ile Gly Thr Ser Leu Phe Arg Glu 
1               5                   10                  15      


Gly Asn Thr Ala Ala Glu Arg Arg Gly Tyr Arg Thr Thr Gln Arg Arg 
            20                  25                  30          


Leu Lys Arg Arg Lys Arg Arg Leu Lys Leu Leu Glu Ala Phe Phe Asp 
        35                  40                  45              


Pro Tyr Ile Phe Glu Val Asp Glu Asn Phe Leu Ala Arg Leu His Glu 
    50                  55                  60                  


Ala Asp Ile Ser Pro Lys Asp Glu Asn Lys Arg Phe Trp Gly Ser Leu 
65                  70                  75                  80  


Leu Phe Pro Asn Glu Lys Asp Ala Asn Phe Tyr Arg Lys Tyr Pro Thr 
                85                  90                  95      


Ile Tyr His Leu Arg Lys Ala Leu Met Glu Glu Asp Arg Lys Phe Asp 
            100                 105                 110         


Val Arg Ala Ile Phe Leu Ala Ile His His Ile Met Lys Tyr Arg Gly 
        115                 120                 125             


Asn Phe Leu Ser Pro Leu Ser Val Asp Ser Phe Lys Gln Glu Gly Leu 
    130                 135                 140                 


Glu Leu Lys Glu Gln Leu Asp Arg Gln Leu Lys Arg Val Ala Glu Leu 
145                 150                 155                 160 


Tyr Asn Glu Leu Asn Thr Asp Val Lys Ile Asn Val Ser Asp Val Glu 
                165                 170                 175     


Thr Ile Gln Asp Ile Ile Ile Asp Lys Asn Lys Ser Arg Arg Asp Lys 
            180                 185                 190         


Ala Gly Ala Leu Lys Lys Leu Leu Ala Val Ser Ser Asp Arg Lys Glu 
        195                 200                 205             


Ile Asn Lys Leu Asn Ala Ser Leu Ala Lys Glu Leu Ser Asn Ala Ile 
    210                 215                 220                 


Leu Gly Met Lys Ala Gln Phe Ala Asn Leu Leu Leu Ala Asp Asp Leu 
225                 230                 235                 240 


Glu Ile Asp Lys Asn Lys Lys Asn Phe Ser Leu Leu Asp Thr Asn Ala 
                245                 250                 255     


Glu Glu Asn Val Ser Gly Leu Leu Glu Leu Val Asn Glu Lys Gln Ala 
            260                 265                 270         


Glu Leu Phe Asp Ala Ile Lys Thr Ile His Asp Gln Ile Ile Leu Asn 
        275                 280                 285             


Glu Ile Val Pro Asp Gly Lys Ser Ile Ser Gln Ser Met Val Asp Lys 
    290                 295                 300                 


Tyr Glu Ala His Arg Glu Gln Leu Gln Val Leu Lys Ala Leu Tyr Glu 
305                 310                 315                 320 


Lys Met Asp Ala Lys Gln Ile Lys Ala Ile Lys Lys Ala Tyr Ser Leu 
                325                 330                 335     


Tyr Ile Asp Gly Lys Asp Pro Lys Ala Lys Lys Val Asp Lys Arg Ala 
            340                 345                 350         


His Gln Asn Arg Ala Asp Phe Tyr Lys Glu Val Lys Lys Val Leu Val 
        355                 360                 365             


Glu Leu Asp Asp Ser Arg Ala Glu Lys Leu Leu Gly Leu Ile Glu Met 
    370                 375                 380                 


Gly Gln Phe Leu Pro Lys Gln Arg Thr Ala Met Asn Gly Val Ile Pro 
385                 390                 395                 400 


Asn Gln Leu His Gln Val Glu Leu Asp Lys Ile Ile Glu Met Gln Ser 
                405                 410                 415     


Lys Tyr Tyr Pro Trp Leu Ala Glu Lys Asn Pro Val Lys Ser His Gln 
            420                 425                 430         


Lys Arg Ala Pro Tyr Lys Leu Asp Glu Leu Leu Thr Phe Arg Val Pro 
        435                 440                 445             


Tyr Tyr Val Gly Pro Met Val Thr Ser Lys Lys Gln Gln Gln Thr Ser 
    450                 455                 460                 


Asn Ser Asn Phe Ala Trp Met Val Arg Lys Gln Glu Gly Glu Ile Thr 
465                 470                 475                 480 


Pro Trp Asn Phe Glu Glu Lys Val Asp Thr Ile Ala Ser Ala Asn Asp 
                485                 490                 495     


Phe Ile Lys Arg Met Thr Ala Lys Asp Thr Tyr Leu Val Gly Glu Asp 
            500                 505                 510         


Val Leu Pro Ala Asn Ser Leu Leu Tyr Gln Arg Tyr Lys Val Leu Asn 
        515                 520                 525             


Glu Leu Asn Asn Ile Lys Val Asn Gly Arg Arg Leu Pro Val Glu Leu 
    530                 535                 540                 


Lys Arg Glu Ala Tyr Val Ala Leu Phe Glu Asn Gly Asp Ser Lys Thr 
545                 550                 555                 560 


Val Ser Ala Lys Cys Phe Lys Asn Trp Leu Asn Val Lys Leu Lys Asn 
                565                 570                 575     


Leu Asn Ser Leu Lys Leu Glu Gly Leu Ser Thr Gly Thr Lys Phe Asp 
            580                 585                 590         


Asn Asn Leu Ser Thr Tyr His Asp Leu Lys Lys Ile Phe Gly Lys Ala 
        595                 600                 605             


Val Asp Asp Ala Gly Arg Gln Lys Asp Phe Glu Gln Ile Ile Glu Trp 
    610                 615                 620                 


Leu Thr Ile Phe Glu Asp Lys Lys Ile Leu Arg Ile Lys Leu Lys Glu 
625                 630                 635                 640 


Ile Glu Trp Leu Thr Glu Glu Asn Ile Thr Gln Leu Thr Arg Leu Asn 
                645                 650                 655     


Tyr Asn Gly Trp Gly Arg Leu Ser Arg Arg Leu Leu Thr Gly Ile Cys 
            660                 665                 670         


Asp Ser Asn Gly Gln Arg Ile Met Asp Trp Leu Trp Asp Thr Thr Asn 
        675                 680                 685             


Asn Phe Met Gln Ile Gln Ser Gln Pro Asp Phe Gln Lys Ala Ile Val 
    690                 695                 700                 


Glu Tyr Asn Ser Asp Lys Leu Glu Gln Gly Asp Ile Glu Gln Ile Leu 
705                 710                 715                 720 


Gly Glu Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Lys Val 
                725                 730                 735     


Ile Lys Val Val Gln Asp Val Glu Lys Ala Met Gly Ala Lys Pro Thr 
            740                 745                 750         


Ser Ile Ser Ile Glu Phe Thr Arg Ser Asp Gly Arg Ser Glu Leu Thr 
        755                 760                 765             


Asn Ser Arg Met Thr Gln Leu Gln Lys Thr Leu Met Ser Leu Ser Glu 
    770                 775                 780                 


Glu Met Thr Asp Gln Ala Leu Leu Ala Glu Phe Lys Asp Phe Gln Ala 
785                 790                 795                 800 


Ser Lys Lys Lys Leu Thr Asn Lys Tyr Tyr Leu Tyr Phe Thr Gln Leu 
                805                 810                 815     


Gly Arg Asp Met Tyr Thr Gly Glu Pro Leu Asn Ile Asp Gln Leu Ser 
            820                 825                 830         


Leu Tyr Ser Asp Gly Asp His Ile Leu Pro Arg Ser Phe Ile Lys Asp 
        835                 840                 845             


Asp Ser Leu Asp Asn Arg Val Leu Thr Lys Arg Ala Val Asn Asn Glu 
    850                 855                 860                 


Lys Ser Asp Thr Val Pro Val Cys Glu Tyr Gly Ala Lys Met Lys Asp 
865                 870                 875                 880 


Phe Trp Asn Asp Leu Tyr Asp Asn Gly Leu Ile Ser Lys Gln Lys Leu 
                885                 890                 895     


Ala Asn Leu Leu Thr Asp Pro Asp Glu Ile Gly Pro Tyr Lys Ala Asn 
            900                 905                 910         


Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu 
        915                 920                 925             


Ser Ala Glu Ile Leu Gln Thr Leu Tyr Gln Asp Thr Lys Ile Ile Glu 
    930                 935                 940                 


Val Arg Gln Lys Tyr Thr His Glu Leu Arg Glu His Phe Asn Phe Val 
945                 950                 955                 960 


Lys Ile Arg Gln Leu Asn Asp Tyr His His Ala Phe Asp Ala Tyr Leu 
                965                 970                 975     


Thr Ala Phe Ser Gly Arg Tyr Leu Tyr Gln Arg Tyr Pro Lys Leu Arg 
            980                 985                 990         


Gly Tyr Phe Val Tyr Gly Asp Tyr  Asn Lys Phe Asp Ala  Lys Lys Asp 
        995                 1000                 1005             


Arg Asn  Tyr Leu Lys Ser Tyr  Asn Phe Leu Tyr Asp  Leu Glu Glu 
    1010                 1015                 1020             


Gly Lys  Thr Asp Gln Ile Val  Asp Asn Val Glu His  Lys Val Phe 
    1025                 1030                 1035             


Met Asn  Arg Gln Asp Ala Ile  Asn Gln Leu Lys Lys  Ala Tyr Glu 
    1040                 1045                 1050             


Tyr Lys  Tyr Met Leu Val Ser  Gln Glu Thr Tyr Thr  Asn Ser Gly 
    1055                 1060                 1065             


Ala Met  Phe Lys Glu Thr Ile  Tyr Pro Val Asn Pro  Lys Gln Lys 
    1070                 1075                 1080             


Leu Ile  Gln Lys Lys Asn Asp  Lys Pro Val Asp Ile  Tyr Gly Gly 
    1085                 1090                 1095             


Tyr Ser  Asn Asn Tyr Asp Ala  Tyr Leu Ser Ile Val  Arg Leu Glu 
    1100                 1105                 1110             


Asp Lys  Lys Gly Ile Lys Phe  Arg Val Val Gly Ile  Pro Met Arg 
    1115                 1120                 1125             


Asn Lys  Ala Glu Leu Asp Asn  Leu Ala Lys Glu Asp  His Asn Gly 
    1130                 1135                 1140             


Tyr Met  Gln Arg Leu Asn Glu  Ile Val Asp Glu Gln  Ile Asn Ser 
    1145                 1150                 1155             


Ser Gly  Lys Lys Lys Thr Phe  Arg Ile Ile Leu Pro  Lys Val His 
    1160                 1165                 1170             


Phe Lys  Gln Leu Val Glu Asp  Asp Ser Gly Lys Phe  Met Leu Gly 
    1175                 1180                 1185             


Ser Ala  Ala Tyr Pro Tyr Asn  Ala Lys Gln Leu Val  Leu Ser Val 
    1190                 1195                 1200             


Lys Ser  Ile Glu Ser Leu Ala  Asn Lys Asp Ala Ser  Asp Arg Glu 
    1205                 1210                 1215             


Leu Val  Ala Val Tyr Glu Glu  Ile Leu Asn Val Val  Asn Asp Lys 
    1220                 1225                 1230             


Phe Thr  Leu Tyr Asp Ala Arg  Gln Phe Arg Asn Lys  Leu Thr Ser 
    1235                 1240                 1245             


Ser Lys  Asp Lys Phe Val Glu  Leu Asn Thr Ala Asp  Lys Val Thr 
    1250                 1255                 1260             


Glu Leu  Leu Asp Ile Leu Asp  Gly Leu His Ala Asn  Pro Val Val 
    1265                 1270                 1275             


Lys Lys  Leu Lys Ile Ile Ser  Gly Ser Pro Phe Gly  Phe Leu Gln 
    1280                 1285                 1290             


Arg Pro  Ser Gly Ile Val Leu  Thr Glu Asn Ala Lys  Leu Ile Tyr 
    1295                 1300                 1305             


Gln Ser  Pro Thr Gly Ile Phe  Glu Arg Val Val Tyr  Leu Arg Asp 
    1310                 1315                 1320             


Leu 
    


<210>  824
<211>  1327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  824

Met Glu Gly Gln Met Lys Asn Asn Gly Asn Asn Leu Gln Gln Gly Asn 
1               5                   10                  15      


Tyr Tyr Leu Gly Leu Asp Val Gly Thr Ser Ser Val Gly Trp Ala Val 
            20                  25                  30          


Thr Asp Thr Asp Tyr Asn Val Leu Lys Phe Arg Gly Lys Ser Met Trp 
        35                  40                  45              


Gly Ala Arg Leu Phe Asp Glu Ala Ser Thr Ala Glu Glu Arg Arg Thr 
    50                  55                  60                  


His Arg Gly Asn Arg Arg Arg Leu Ala Arg Arg Lys Tyr Arg Leu Leu 
65                  70                  75                  80  


Leu Leu Glu Gln Leu Phe Glu Lys Glu Ile Arg Lys Ile Asp Asp Asn 
                85                  90                  95      


Phe Phe Val Arg Leu His Glu Ser Asn Leu Trp Ala Asp Asp Lys Ser 
            100                 105                 110         


Lys Pro Ser Lys Phe Leu Leu Phe Asn Asp Thr Asn Phe Thr Asp Lys 
        115                 120                 125             


Asp Tyr Leu Lys Lys Tyr Pro Thr Ile Tyr His Leu Arg Ser Asp Leu 
    130                 135                 140                 


Ile His Asn Ser Thr Glu His Asp Ile Arg Leu Val Phe Leu Ala Leu 
145                 150                 155                 160 


His His Leu Ile Lys Tyr Arg Gly His Phe Ile Tyr Asp Asn Ser Ala 
                165                 170                 175     


Asn Gly Asp Val Lys Thr Leu Asp Glu Ala Val Ser Asp Phe Glu Glu 
            180                 185                 190         


Tyr Leu Asn Glu Asn Asp Ile Glu Phe Asn Ile Glu Asn Lys Lys Glu 
        195                 200                 205             


Phe Ile Asn Val Leu Ser Asp Lys His Leu Thr Lys Lys Glu Lys Lys 
    210                 215                 220                 


Ile Ser Leu Lys Lys Leu Tyr Gly Asp Ile Thr Asp Ser Glu Asn Ile 
225                 230                 235                 240 


Asn Ile Ser Val Leu Ile Glu Met Leu Ser Gly Ser Ser Ile Ser Leu 
                245                 250                 255     


Ser Asn Leu Phe Lys Asp Ile Glu Phe Asp Gly Lys Gln Asn Leu Ser 
            260                 265                 270         


Leu Asp Ser Asp Ile Glu Glu Thr Leu Asn Asp Val Val Asp Ile Leu 
        275                 280                 285             


Gly Asp Asn Ile Asp Leu Leu Ile His Ala Lys Glu Val Tyr Asp Ile 
    290                 295                 300                 


Ala Val Leu Thr Ser Ser Leu Gly Lys His Lys Tyr Leu Cys Asp Ala 
305                 310                 315                 320 


Lys Val Glu Leu Phe Glu Lys Asn Lys Lys Asp Leu Met Ile Leu Lys 
                325                 330                 335     


Lys Tyr Ile Lys Lys Asn His Pro Glu Asp Tyr Lys Lys Ile Phe Ser 
            340                 345                 350         


Ser Pro Thr Glu Lys Lys Asn Tyr Ala Ala Tyr Ser Gln Thr Asn Ser 
        355                 360                 365             


Lys Asn Val Cys Ser Gln Glu Glu Phe Cys Leu Phe Ile Lys Pro Tyr 
    370                 375                 380                 


Ile Arg Asp Met Val Lys Ser Glu Asn Glu Asp Glu Val Arg Ile Ala 
385                 390                 395                 400 


Lys Glu Val Glu Asp Lys Ser Phe Leu Thr Lys Leu Lys Gly Thr Asn 
                405                 410                 415     


Asn Ser Val Val Pro Tyr Gln Ile His Glu Arg Glu Leu Asn Gln Ile 
            420                 425                 430         


Leu Lys Asn Ile Val Ala Tyr Leu Pro Phe Met Asn Asp Glu Gln Glu 
        435                 440                 445             


Asp Ile Ser Val Val Asp Lys Ile Lys Leu Ile Phe Lys Phe Lys Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Asn Thr Lys Ser Thr Arg Ser Trp Val 
465                 470                 475                 480 


Tyr Arg Ser Asp Glu Lys Ile Tyr Pro Trp Asn Phe Ser Asn Val Ile 
                485                 490                 495     


Asp Leu Asp Lys Thr Ala His Glu Phe Met Asn Arg Leu Ile Gly Arg 
            500                 505                 510         


Cys Thr Tyr Thr Asn Asp Pro Val Leu Pro Met Asp Ser Leu Leu Tyr 
        515                 520                 525             


Ser Lys Tyr Asn Val Leu Asn Glu Ile Asn Pro Ile Lys Val Asn Gly 
    530                 535                 540                 


Lys Ala Ile Pro Val Glu Val Lys Gln Ala Ile Tyr Thr Asp Leu Phe 
545                 550                 555                 560 


Glu Asn Ser Lys Lys Lys Val Thr Arg Lys Ser Ile Tyr Ile Tyr Leu 
                565                 570                 575     


Leu Lys Asn Gly Tyr Ile Glu Lys Glu Asp Ile Val Ser Gly Ile Asp 
            580                 585                 590         


Ile Glu Ile Lys Ser Lys Leu Lys Ser His His Asp Phe Thr Gln Ile 
        595                 600                 605             


Val Gln Glu Asn Lys Cys Thr Pro Glu Glu Ile Glu Arg Ile Ile Lys 
    610                 615                 620                 


Gly Ile Leu Val Tyr Ser Asp Asp Lys Ser Met Leu Arg Arg Trp Leu 
625                 630                 635                 640 


Lys Asn Asn Ile Lys Gly Leu Ser Glu Asn Asp Val Lys Tyr Leu Ala 
                645                 650                 655     


Lys Leu Asn Tyr Lys Glu Trp Gly Arg Leu Ser Lys Thr Leu Leu Thr 
            660                 665                 670         


Asp Ile Tyr Thr Ile Asn Pro Glu Asp Gly Glu Ala Cys Ser Ile Leu 
        675                 680                 685             


Asp Ile Met Trp Asn Thr Asn Ala Thr Leu Met Glu Ile Leu Ser Asn 
    690                 695                 700                 


Glu Lys Tyr Gln Phe Lys Gln Asn Ile Glu Asn Tyr Lys Ala Glu Asn 
705                 710                 715                 720 


Tyr Asp Glu Lys Gln Asn Leu His Glu Glu Leu Asp Asp Met Tyr Ile 
                725                 730                 735     


Ser Pro Ala Ala Arg Arg Ser Ile Trp Gln Ala Leu Arg Ile Val Asp 
            740                 745                 750         


Glu Ile Val Asp Ile Lys Lys Ser Ala Pro Lys Lys Ile Phe Ile Glu 
        755                 760                 765             


Met Ala Arg Glu Lys Lys Ser Ala Met Lys Lys Lys Arg Thr Glu Ser 
    770                 775                 780                 


Arg Lys Asp Thr Leu Leu Glu Leu Tyr Lys Ser Cys Lys Ser Gln Ala 
785                 790                 795                 800 


Asp Gly Phe Tyr Asp Glu Glu Leu Phe Glu Lys Leu Ser Asn Glu Ser 
                805                 810                 815     


Asn Ser Arg Leu Arg Arg Asp Gln Leu Tyr Leu Tyr Tyr Thr Gln Met 
            820                 825                 830         


Gly Arg Ser Met Tyr Thr Gly Lys Arg Ile Asp Phe Asp Lys Leu Ile 
        835                 840                 845             


Asn Asp Lys Asn Thr Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Lys 
    850                 855                 860                 


Ile Lys Asp Asp Ser Ile Thr Asn Arg Val Leu Val Glu Lys Asp Ile 
865                 870                 875                 880 


Asn Gly Glu Lys Thr Asp Ile Tyr Pro Ile Ser Glu Asp Ile Arg Gln 
                885                 890                 895     


Lys Met Gln Pro Phe Trp Lys Ile Leu Lys Glu Lys Gly Leu Ile Asn 
            900                 905                 910         


Glu Glu Lys Tyr Lys Arg Leu Thr Arg Asn Tyr Glu Leu Thr Asp Glu 
        915                 920                 925             


Glu Leu Ser Ser Phe Val Ala Arg Gln Leu Val Glu Thr Gln Gln Ser 
    930                 935                 940                 


Thr Lys Ala Leu Ala Thr Leu Leu Lys Lys Glu Tyr Pro Ser Ala Lys 
945                 950                 955                 960 


Ile Val Tyr Ser Lys Ala Gly Asn Val Ser Glu Phe Arg Asn Arg Lys 
                965                 970                 975     


Asp Lys Glu Leu Pro Lys Phe Arg Glu Ile Asn Asp Leu His His Ala 
            980                 985                 990         


Lys Asp Ala Tyr Leu Asn Ile Val  Val Gly Asn Val Tyr  Asp Thr Lys 
        995                 1000                 1005             


Phe Thr  Glu Lys Phe Phe Asn  Asn Ile Arg Asn Glu  Asn Tyr Ser 
    1010                 1015                 1020             


Leu Lys  Arg Val Phe Asp Phe  Ser Val Pro Gly Ala  Trp Asp Ala 
    1025                 1030                 1035             


Lys Gly  Ser Thr Phe Asn Thr  Ile Lys Lys Tyr Met  Ala Lys Asn 
    1040                 1045                 1050             


Asn Pro  Ile Ile Ala Phe Ala  Pro Tyr Glu Val Lys  Gly Glu Leu 
    1055                 1060                 1065             


Phe Asp  Gln Gln Ile Val Pro  Lys Gly Lys Gly Gln  Phe Pro Ile 
    1070                 1075                 1080             


Lys Gln  Gly Lys Asp Ile Glu  Lys Tyr Gly Gly Tyr  Asn Lys Leu 
    1085                 1090                 1095             


Ser Ser  Ala Phe Leu Phe Ala  Val Glu Tyr Lys Gly  Lys Lys Ala 
    1100                 1105                 1110             


Arg Glu  Arg Ser Leu Glu Thr  Val Tyr Ile Lys Asp  Val Glu Leu 
    1115                 1120                 1125             


Tyr Leu  Gln Asp Pro Ile Lys  Tyr Cys Glu Ser Val  Leu Gly Leu 
    1130                 1135                 1140             


Lys Glu  Pro Gln Ile Ile Lys  Pro Lys Ile Leu Met  Gly Ser Leu 
    1145                 1150                 1155             


Phe Ser  Ile Asn Asn Lys Lys  Leu Val Val Thr Gly  Arg Ser Gly 
    1160                 1165                 1170             


Lys Gln  Tyr Val Cys His His  Ile Tyr Gln Leu Ser  Ile Asn Asp 
    1175                 1180                 1185             


Glu Asp  Ser Gln Tyr Leu Lys  Asn Ile Ala Lys Tyr  Leu Gln Glu 
    1190                 1195                 1200             


Glu Pro  Asp Gly Asn Ile Glu  Arg Gln Asn Ile Leu  Asn Ile Thr 
    1205                 1210                 1215             


Ser Val  Asn Asn Ile Lys Leu  Phe Asp Val Leu Cys  Thr Lys Phe 
    1220                 1225                 1230             


Asn Ser  Asn Thr Tyr Glu Ile  Ile Leu Asn Ser Leu  Lys Asn Asp 
    1235                 1240                 1245             


Val Asn  Glu Gly Arg Glu Lys  Phe Ser Glu Leu Asp  Ile Leu Glu 
    1250                 1255                 1260             


Gln Cys  Asn Ile Leu Leu Gln  Leu Leu Lys Ala Phe  Lys Cys Asn 
    1265                 1270                 1275             


Arg Glu  Ser Ser Asn Leu Glu  Lys Leu Asn Asn Lys  Lys Gln Ala 
    1280                 1285                 1290             


Gly Val  Ile Val Ile Pro His  Leu Phe Thr Lys Cys  Ser Val Phe 
    1295                 1300                 1305             


Lys Val  Ile His Gln Ser Ile  Thr Gly Leu Phe Glu  Lys Glu Met 
    1310                 1315                 1320             


Asp Leu  Leu Lys 
    1325         


<210>  825
<211>  1327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  825

Met Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Gly Ser Val Gly 
1               5                   10                  15      


Trp Val Cys Ile Asp Glu Asn Tyr Gln Ile Leu Arg Tyr Asn Asn Arg 
            20                  25                  30          


Ala Ala Phe Gly Val His Glu Phe Glu Thr Ala His Val Ala Glu Glu 
        35                  40                  45              


Arg Arg Ile Lys Arg Gly Ala Arg Arg Arg Tyr Asn Arg Arg Lys Lys 
    50                  55                  60                  


Arg Ile Gln Leu Leu Gln Ser Leu Phe Val Pro His Met Gln Gln Lys 
65                  70                  75                  80  


Ala Phe Phe Ala Pro Thr Ile Thr Glu His Tyr Trp Lys Asn Asp Asn 
                85                  90                  95      


Gln Phe Glu Asn Arg Thr Leu Ser Glu Val Leu Met Ser Leu Gly Met 
            100                 105                 110         


Asn Pro Asn Thr Tyr Pro Thr Ile Phe His Leu Arg His Glu Leu Ile 
        115                 120                 125             


Glu Ser Ser Val Lys Lys Asp Ala Arg Leu Ile Tyr Leu Ala Leu His 
    130                 135                 140                 


Asn Leu Val Lys Phe Arg Gly His Phe Leu Gln Glu Gly Ser Thr Trp 
145                 150                 155                 160 


Asn Ala Asn Met Ser Asp Ala Asn Leu Ser Glu Gln Phe Glu Gln Leu 
                165                 170                 175     


Leu His Ile Tyr Thr Glu Met Glu Glu Leu Pro Gln Val Ser Leu Thr 
            180                 185                 190         


Ala Gln Gln Ser Thr Asp Val Glu His Ile Leu Leu Asn Asn His Ile 
        195                 200                 205             


Ser Lys Ala Glu Arg Ala Arg Gln Leu Thr Ala Leu Phe Glu Arg Thr 
    210                 215                 220                 


Tyr Glu Pro Phe Phe Lys Leu Ile Val Gly Leu Asp Ile Ala Ala Ala 
225                 230                 235                 240 


Gln Leu Phe Lys Gln Ser Glu Gln Gln Leu Leu Tyr Lys Glu Ser Lys 
                245                 250                 255     


Thr Lys Val Asn Leu Thr Ala Glu Thr Leu Pro Glu Val Leu Gln Thr 
            260                 265                 270         


Met Leu Glu Pro Glu Val Ala Phe Ile Glu Ala Val Gln Pro Phe Tyr 
        275                 280                 285             


Gln Gln Thr Val Leu Phe Asp Leu Leu Lys Gly Gln Ser Tyr Val Ala 
    290                 295                 300                 


Ala Ala Lys Val Glu Ala Phe Asn Gln Tyr Glu Arg Asp Leu Ala Leu 
305                 310                 315                 320 


Leu Lys Ser Val Ile Asn Glu Val Gly Ser Glu Lys Gln Tyr Arg Ala 
                325                 330                 335     


Phe Phe Ile Thr Ser Lys Gln Lys Lys Arg Gln Phe Glu Gln Lys Pro 
            340                 345                 350         


Asn Met Glu Leu Leu Cys His Leu Asp Arg Phe Asn Arg Glu Lys Lys 
        355                 360                 365             


Tyr Glu Glu Thr Phe Tyr Lys Glu Val Ser Lys Leu Leu Thr Ala Leu 
    370                 375                 380                 


Ile Lys Glu Ala Thr Gly Glu Val Lys Glu His Ile Glu Gln Ile Ile 
385                 390                 395                 400 


Gln Ala Met Glu Ser Gln Ser Phe Leu Val Lys Gln Lys Gly Ile Gln 
                405                 410                 415     


Asn Ala Ser Ile Pro Tyr Gln Asn Ser Leu Tyr Glu Ala Glu Met Ile 
            420                 425                 430         


Leu Arg Asn Gln Gln Ala Tyr Tyr Pro Trp Ile Thr Asp Glu Phe Ile 
        435                 440                 445             


Glu Lys Val Lys Gln Leu Ile Ala Phe Arg Ile Pro Tyr Tyr Ile Gly 
    450                 455                 460                 


Pro Leu Val Gln Gly Asp Glu Gln Pro Phe Gly Trp Leu Gln Arg Gln 
465                 470                 475                 480 


Ala Asp Gly Asn Ile Thr Pro Trp Asn Phe Asp Glu Lys Val Asn Lys 
                485                 490                 495     


Ala Ala Ser Ala Glu Ser Phe Ile Thr Arg Met Thr Lys Asn Cys Thr 
            500                 505                 510         


Tyr Leu Lys Glu Glu Lys Val Leu Pro Lys Gln Ser Leu Met Tyr Glu 
        515                 520                 525             


Lys Phe Thr Leu Leu Asn Glu Leu Asn Ser Val Gln Ile Arg Ser Ser 
    530                 535                 540                 


Leu Glu Gln Pro His Lys Lys His Arg Leu Cys Glu Asp Met Lys Gln 
545                 550                 555                 560 


Trp Ile Ile Glu His Val Phe Lys Lys Thr Lys Thr Val Thr His Lys 
                565                 570                 575     


Thr Leu Ile Gln Ala Leu Lys Lys Ser Pro Tyr Lys Asp Glu Leu Tyr 
            580                 585                 590         


Asp Glu Ser Thr Gly Asn Trp Lys Asp Val Tyr Gly Thr Gln Lys Glu 
        595                 600                 605             


Asp Arg Phe Ala Ser Ser Leu Ser Thr Tyr Ile Ser Leu Lys Glu Ile 
    610                 615                 620                 


Val Gly Asp Ile Val Asp Asn Ala Phe Met Glu Glu Ile Ile Tyr Trp 
625                 630                 635                 640 


Leu Thr Val Phe Thr Asp Arg Asp Ile Ile Thr Leu Lys Met Asn Asp 
                645                 650                 655     


Lys Tyr Pro Phe Ile Thr Glu Gln Gln Ile Gln Arg Leu Leu Ser Leu 
            660                 665                 670         


Asn Ile Ser Gly Trp Gly Gln Leu Ser Gln Arg Leu Leu Gln Ser Ile 
        675                 680                 685             


Pro Val Asp Asp Met Asn Arg Ser Val Ile Glu Tyr Met Glu Lys His 
    690                 695                 700                 


Ala Val Asn Phe Met Glu Leu Leu Ser Pro Arg Gln Ser Asp Leu Ala 
705                 710                 715                 720 


Tyr Lys Ile Glu Gln Val Asn Lys Gln His Ser Lys Lys Ile His Lys 
                725                 730                 735     


Ile Arg Tyr Glu Asp Ile Glu Gln Leu Thr Gly Ser Pro Ala Leu Lys 
            740                 745                 750         


Arg Gly Ile Trp Arg Ser Val Lys Ile Ile Glu Glu Leu Thr Ala Ile 
        755                 760                 765             


Phe Gly Glu Pro Lys Asn Ile Met Ile Glu Val Ala Arg Glu Asp Gln 
    770                 775                 780                 


Ala Ser His Arg Thr Lys Ser Arg Lys Glu Gln Trp Glu Ser Ile Gly 
785                 790                 795                 800 


Lys Ser Leu Ala Lys Glu Glu Lys Glu Leu Lys Ala Phe Phe Lys Glu 
                805                 810                 815     


Met Ala Gln Tyr Pro Lys Gln Gln Phe Asp Arg Thr Arg Phe Trp Leu 
            820                 825                 830         


Tyr Val Thr Gln Gln Gly Lys Cys Leu Tyr Thr Gly Lys Thr Leu Arg 
        835                 840                 845             


Leu Glu Asp Leu Ser Ser Tyr Glu Ile Asp His Ile Leu Pro Arg Asn 
    850                 855                 860                 


Phe Val Lys Asp Asp Ser Ile Asp Asn Leu Ala Leu Val Tyr Pro Met 
865                 870                 875                 880 


Ala Asn Gln Glu Lys Asn Lys Ala Gly Asn Asn Lys Met Pro Leu Glu 
                885                 890                 895     


Ile Ile Asp Ala Lys Gln Gln Pro Ala Met Met Ala His Trp Lys Arg 
            900                 905                 910         


Leu Asn Gln Leu Lys Leu Ile Ser Asp Ser Lys Leu His Lys Leu Met 
        915                 920                 925             


Lys Pro Ser Phe Asn Asp Val Asp Lys Glu Asn Phe Ile Ala Arg Gln 
    930                 935                 940                 


Leu Val Glu Thr Arg Gln Ile Ile Lys His Val Arg Asp Leu Leu Gln 
945                 950                 955                 960 


Glu Arg Phe Glu His Thr Asp Ile His Val Val Lys Ala Lys Met Val 
                965                 970                 975     


Ser Gln Phe Arg Lys Phe Ser Arg Ile Pro Lys Ile Arg Asp Tyr Asn 
            980                 985                 990         


Asn Lys His His Ala Met Asp Ala  Leu Phe Thr Thr Val  Leu Ile Gln 
        995                 1000                 1005             


Phe Ile  Leu Gln Lys Tyr Gly  Lys Asn Phe Leu Gln  Phe Asp Leu 
    1010                 1015                 1020             


Gln His  Lys Asp Ile Ser Lys  Lys Trp Ser Lys Ile  Ala Gln Ser 
    1025                 1030                 1035             


Thr Lys  His Phe Phe Leu Phe  Gln Gln Phe Ala Glu  Gln Gln Ile 
    1040                 1045                 1050             


Lys Ser  Pro Val Thr Lys Glu  Leu Val Ser Gly Val  Gln Tyr Phe 
    1055                 1060                 1065             


Glu His  Ile Phe Tyr Glu Gln  Ser Trp Gln Thr Thr  Lys Met Thr 
    1070                 1075                 1080             


Gln Thr  Ile Asp Ser Asn Phe  Tyr Lys Glu Thr Ile  Phe Ser Pro 
    1085                 1090                 1095             


Lys Leu  Lys Thr Ala Lys Tyr  Thr Ser Ala Lys Ala  Glu Lys Phe 
    1100                 1105                 1110             


Val His  Asp Ala Ile Val Lys  Tyr Ala Val Cys Gly  Ile Gln Tyr 
    1115                 1120                 1125             


Thr Ser  Leu Lys Lys Asn Lys  Glu Gln His His Val  Leu Leu Thr 
    1130                 1135                 1140             


Asp Ile  Asn Val Ile Glu His  Tyr Gln Leu Lys Glu  Ala Ser Leu 
    1145                 1150                 1155             


Asn Glu  Leu Ala Leu His Leu  Ala Lys Arg Glu Ser  Lys Asp Asp 
    1160                 1165                 1170             


Val Ile  Asp Ala Gln Met Leu  Val Val Ile Pro Lys  Tyr Lys Lys 
    1175                 1180                 1185             


Ile Met  Leu Asn Gly Ser Pro  Tyr Tyr Cys Ile Ser  Ser Asp Glu 
    1190                 1195                 1200             


Arg His  Ile Ala Thr Gln Phe  Val Leu Arg Lys Asp  Leu Leu Glu 
    1205                 1210                 1215             


Ala Val  Leu His Val Asn Asp  Glu Thr Ser Val Asp  Glu Leu Arg 
    1220                 1225                 1230             


Thr Leu  Phe Arg Ser Ile Val  Glu Glu Met Met Thr  Gln Tyr Lys 
    1235                 1240                 1245             


Val Tyr  Asn Lys Asp Thr Ile  Arg Asn Lys Ile Asp  Ala Phe Ile 
    1250                 1255                 1260             


Glu Thr  Glu Leu Ile Asp Val  Glu Thr Phe Gln Val  Gly Phe Thr 
    1265                 1270                 1275             


Glu Ile  Lys Lys Met Val Ala  Ala Asn Ala Gln Arg  Ser Asp Lys 
    1280                 1285                 1290             


Phe Gly  Gly Arg Leu Arg Gln  Val Met Lys Val Glu  Asp Ile Tyr 
    1295                 1300                 1305             


Ile Ile  Asp Glu Ser Ile Thr  Gly Leu Arg Tyr Arg  Lys Pro Arg 
    1310                 1315                 1320             


Lys Leu  Val Lys 
    1325         


<210>  826
<211>  1327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  826

Met Lys Lys Asn Tyr Thr Ile Gly Leu Asp Ile Gly Thr Ala Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Glu Asp Tyr Glu Leu Ile Lys Arg Lys Met 
            20                  25                  30          


Lys Ile Ser Gly Asn Thr Gln Lys Lys Ala Val Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Glu Gln Gly Glu Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Thr Arg Arg Arg Ile Ala Arg Arg Arg Gln Arg Ile Gln 
65                  70                  75                  80  


Tyr Leu Arg Thr Ile Phe Asp Glu Gly Met Asn Gln Val Asp Ala Asn 
                85                  90                  95      


Phe Phe Ala Arg Leu Asp Glu Ser Phe Ser Ile Ile Asp Glu Lys Glu 
            100                 105                 110         


Asn Glu Arg His Pro Ile Phe Gly Asn Val Ala Glu Glu Ala Ala Tyr 
        115                 120                 125             


His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Glu His Leu Ala Asn 
    130                 135                 140                 


Ser Ser Glu Gln Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     


Glu Asn Ser Ser Val Ser Gly Thr Phe Glu Gln Phe Ile Lys Val Tyr 
            180                 185                 190         


Asn Glu Thr Phe Asn Val Glu Lys Ala Leu Asp Leu Thr Val Asp Leu 
        195                 200                 205             


Asp Gly Ile Ala Glu Gln Lys Ile Ser Arg Met Lys Arg Ala Glu Leu 
    210                 215                 220                 


Ile Leu Ser Leu Phe Pro Glu Glu Lys Ser Thr Gly Asp Phe Ala Gln 
225                 230                 235                 240 


Phe Ile Lys Met Ile Val Gly Asn Gln Gly Asn Val Lys Lys Thr Phe 
                245                 250                 255     


Ser Leu Asn Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         


Glu Asn Leu Glu Thr Leu Leu Ala Glu Ile Gly Glu Asp Phe Arg Ser 
        275                 280                 285             


Val Phe Asp Ala Ala Lys Ser Val Tyr Asp Ala Ile Ser Leu Ala Asn 
    290                 295                 300                 


Ile Leu Lys Val Thr Asp Ala Thr Thr Arg Ala Lys Leu Ser Ser Ser 
305                 310                 315                 320 


Met Val Val Arg Phe Thr Asp His Lys Glu Asp Leu Lys Thr Leu Lys 
                325                 330                 335     


Arg Phe Val Arg Glu Asn Leu Pro Asp Glu Tyr Asp Asp Leu Phe Lys 
            340                 345                 350         


Asn Lys Lys Val Ala Gly Tyr Ala Gly Tyr Ile Asp Gly Asn Glu Thr 
        355                 360                 365             


Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Lys Thr Leu Ala Lys Ala Thr 
    370                 375                 380                 


Gly Ala Glu Gly Phe Leu Ala Lys Met Glu Gln Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro Tyr Gln Leu His Leu 
                405                 410                 415     


Glu Glu Leu Lys Ala Ile Ile Lys Asn Gln Lys Ser Tyr Tyr Ser Phe 
            420                 425                 430         


Leu Asp Glu Glu Lys Ile Ser Gln Leu Met Thr Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Leu Ala Lys Glu Lys Gly Gln Phe Ala Trp Leu Thr 
    450                 455                 460                 


Arg Lys Glu Met Gly Lys Ile Thr Pro Trp Asn Leu Asn Glu Lys Val 
465                 470                 475                 480 


Asp Ile Glu Lys Ser Ala Thr Asp Phe Val Glu Arg Met Thr Asn Asn 
                485                 490                 495     


Asp Ser Tyr Leu Pro Met Glu Lys Val Leu Pro Lys His Ser Leu Leu 
            500                 505                 510         


Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Arg Tyr Ile 
        515                 520                 525             


Asp Asp Asn Gly Arg Ala Gln Asn Phe Ser Ser Lys Glu Lys Arg Gln 
    530                 535                 540                 


Ile Ile Asn Glu Leu Phe Lys Gln Gln Arg Lys Val Lys Lys Glu Met 
545                 550                 555                 560 


Leu Glu Ala Phe Leu Lys Asn Glu Tyr Gly Ile Glu Asn Pro Lys Val 
                565                 570                 575     


Glu Gly Ile Glu Lys Ser Phe Asn Ala Cys Leu Gly Thr Tyr His Asp 
            580                 585                 590         


Leu Ile Lys Leu Gly Ile Arg Pro Glu Leu Phe Glu Gln Pro Glu His 
        595                 600                 605             


Glu Gln Gln Phe Glu Gln Ile Val Lys Ile Leu Thr Val Phe Glu Asp 
    610                 615                 620                 


Arg Lys Met Arg Arg Lys Gln Leu Glu Lys Phe Ser Asn Ile Leu Thr 
625                 630                 635                 640 


Glu Glu Glu Gln Lys Gln Leu Glu Arg Lys His Tyr Lys Gly Trp Gly 
                645                 650                 655     


Arg Leu Ser Ala Lys Leu Ile His Gly Ile Val Asp Gln Lys Thr Gln 
            660                 665                 670         


Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Asp Leu Pro Lys Asn 
        675                 680                 685             


Arg Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Glu Asn Leu Ser Phe 
    690                 695                 700                 


Lys Glu Glu Ile Glu Lys Ile Ala Phe Asp Asn Asp Lys Ser Thr Glu 
705                 710                 715                 720 


Glu Ile Val Gln Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Glu Glu Ile Ile Asp Ile Met Gly Glu 
            740                 745                 750         


Leu Pro Thr Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Gln Gly Asn Arg Ala Ser Lys Ala Arg Met Lys Tyr Leu Glu Glu 
    770                 775                 780                 


Ser Ile Lys Lys Leu Gly Ser Ser Ile Leu Glu Asp Glu Pro Ile Ser 
785                 790                 795                 800 


Lys Asp Ala Asn Leu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Thr Gly Asn Glu Leu Asp Ile Asn Asn 
            820                 825                 830         


Leu Ser Ser Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Val Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Thr Ser Ser Met Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asn Thr Val Pro Ala Glu Ser Val Val Lys Lys Met Arg 
865                 870                 875                 880 


Pro Thr Trp Glu Arg Leu Leu Ala Ser Gly Leu Ile Ser Lys Lys Lys 
                885                 890                 895     


Phe Ser Tyr Leu Thr Lys Ala Thr Asn Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         


Lys Ala His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys Asn Val Ala Gln Ile Leu His Gln Lys Tyr Asn Asn Glu Gln Leu 
    930                 935                 940                 


Ser Glu Lys Pro Val Arg Val Ile Thr Leu Lys Ser Ala Leu Ala Ser 
945                 950                 955                 960 


Gln Phe Arg Lys Asp Phe Ser Leu Tyr Lys Ile Arg Glu Leu Asn Asp 
                965                 970                 975     


Tyr His His Ala Gln Asp Ala Tyr Leu Asn Gly Val Ile Ala Gln Ala 
            980                 985                 990         


Leu Leu Lys Val Tyr Pro Lys Leu  Glu Pro Glu Phe Val  Tyr Gly Glu 
        995                 1000                 1005             


Tyr Gln  Lys Val Ser Ile Arg  Ala Leu Asn Lys Ala  Thr Ala Lys 
    1010                 1015                 1020             


Lys Glu  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Thr  Ser Asp Glu 
    1025                 1030                 1035             


Met Val  Ala Asn Glu Glu Thr  Gly Glu Val Leu Trp  Asn Arg Gln 
    1040                 1045                 1050             


Arg Asp  Ile Lys Thr Ile Lys  Lys Val Met Asn Tyr  His Gln Met 
    1055                 1060                 1065             


Asn Ile  Val Lys Lys Val Glu  Ile Gln Thr Gly Ala  Phe Thr Lys 
    1070                 1075                 1080             


Glu Ser  Ile Leu Pro Lys Gly  Pro Ser Lys Lys Leu  Ile Ala Arg 
    1085                 1090                 1095             


Lys Asn  Asn Trp Ala Pro Val  Asn Tyr Gly Gly Val  Asp Ser Pro 
    1100                 1105                 1110             


Thr Val  Ala Tyr Ser Val Ile  Ile Thr His Glu Lys  Gly Lys Ala 
    1115                 1120                 1125             


Ala Lys  Val Val Gln Gln Leu  Val Gly Ile Lys Ile  Leu Glu Arg 
    1130                 1135                 1140             


Gln Ala  Phe Glu Gln Asp Glu  Val Ala Phe Leu Glu  Glu Lys Gly 
    1145                 1150                 1155             


Phe Ile  His Pro Lys Val Gln  Leu Lys Leu Pro Lys  Tyr Ser Leu 
    1160                 1165                 1170             


Tyr Gln  Phe Ala Asp Gly Arg  Arg Arg Leu Leu Ala  Ser Ala Glu 
    1175                 1180                 1185             


Glu Ser  Gln Lys Gly Asn Gln  Met Val Leu Pro Val  His Leu Ile 
    1190                 1195                 1200             


Glu Leu  Leu Tyr His Ala Lys  His Val Ser Asp Ser  Ser Gly Lys 
    1205                 1210                 1215             


Ser Leu  Glu Tyr Leu Asn Glu  His Arg His Glu Phe  Ala Glu Leu 
    1220                 1225                 1230             


Leu Glu  Ala Ile Leu Gln Phe  Thr Glu Gln Tyr Ile  Asp Ala Gly 
    1235                 1240                 1245             


Lys Asn  Gln Lys Lys Val Arg  Asp Leu Tyr Glu Lys  Asn Gln Asp 
    1250                 1255                 1260             


Ala Asp  Met Arg Glu Leu Ala  Ser Ser Phe Ile Gln  Leu Leu Gln 
    1265                 1270                 1275             


Leu Asn  Lys Gln Gly Ala Pro  Ala Asp Phe Lys Phe  Phe Gly Glu 
    1280                 1285                 1290             


Thr Ile  Pro Arg Arg Arg Tyr  Lys Asn Thr Ala Glu  Ile Val Asp 
    1295                 1300                 1305             


Ala Thr  Phe Ile Asn Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Gln 
    1310                 1315                 1320             


Lys Arg  Leu Val 
    1325         


<210>  827
<211>  1327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  827

Met Thr Glu Gln Gln Gln Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Ser Ser Ile Gly Trp Ala Val Thr Asp Gln Asn Asp Lys Leu Leu His 
            20                  25                  30          


Ile Arg Gly His Asn Gly Ile Gly Val Arg Leu Phe Lys Glu Gly Glu 
        35                  40                  45              


Ser Ala Ala Glu Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Arg Arg Trp Arg Leu Arg Phe Leu Asp Arg Ile Phe Asp Ala Glu 
65                  70                  75                  80  


Leu Ala Lys Val Asp Pro Asn Phe Leu Ala Arg Leu Lys Gln Ser Asn 
                85                  90                  95      


Ile Ser Pro Leu Asp Pro Asn Lys Gln Met Phe Gly Asn Ile Leu Phe 
            100                 105                 110         


Asp Asp Pro Gln Phe Asp Asp Gln Lys Phe His Gln Gln Tyr Pro Thr 
        115                 120                 125             


Ile Tyr His Leu Arg Ser Ala Leu Ala Gln Pro Asp Ala Pro Gln Ala 
    130                 135                 140                 


Asp Val Arg Leu Ile Tyr Leu Ala Leu His His Met Ile Lys Phe Arg 
145                 150                 155                 160 


Gly His Phe Leu Asn Gly Ala Ala Val Glu Lys Phe His Gly Gly Gln 
                165                 170                 175     


Leu His Leu Glu Ser Glu Phe Gln Glu Leu Asn Gln Ile Phe Ser Asn 
            180                 185                 190         


Gln Gln Arg Asp His Val Gln Leu Gln Thr Ser Ser Leu Asp Glu Val 
        195                 200                 205             


Ala Thr Ile Leu Lys Asp Asn Gln Arg Thr Pro Ser Asp Arg Gln Arg 
    210                 215                 220                 


Glu Leu Ser Ala Arg Leu Phe Val Pro Val Asp Lys Thr Ile Asp Lys 
225                 230                 235                 240 


Glu Asn Lys Lys Leu Ala Thr Glu Leu Leu Lys Ala Val Leu Gly Leu 
                245                 250                 255     


Lys Ala Lys Phe Asp Val Ile Leu Gly Val Glu Ala Thr Asp Pro Gln 
            260                 265                 270         


Ala Phe Lys Leu Gln Phe Ser Ser Asp Asp Phe Asp Asp Lys Ile Ala 
        275                 280                 285             


Glu Leu Ala Ser Glu Leu Thr Asp Glu Ala Asn Glu Ile Leu Thr Val 
    290                 295                 300                 


Leu Gln Arg Leu Tyr Phe Ser Leu Asn Leu Ala Asp Ile Leu Thr Asn 
305                 310                 315                 320 


Arg Gln Thr Lys Gln Val Ala Asp Ser Ile Ser Thr Ala Met Ile Asn 
                325                 330                 335     


Arg Tyr Asp Asp His Lys His His Leu Lys Met Leu Lys Glu Leu Glu 
            340                 345                 350         


Ala Gln Val Thr Gln Glu Gln Arg Asp Gly Leu Lys Lys Ala Tyr Asn 
        355                 360                 365             


Glu Tyr Ile Asp Gly Asp Lys Pro Thr Ile Asp Thr Phe Tyr Thr Asn 
    370                 375                 380                 


Val Lys Lys Asn Leu Asp Asp Ser Glu Leu Ala Asn Gln Ile Lys Ser 
385                 390                 395                 400 


Leu Ile Asp Leu Gln Ala Phe Met Pro Lys Gln Arg Ser Lys Glu Asn 
                405                 410                 415     


Gly Ser Ile Pro His Gln Leu His Gln Arg Glu Leu Asp Gln Leu Ile 
            420                 425                 430         


Glu Val Gln Gly Lys Tyr Tyr Pro Trp Leu Leu Ala Ala Lys Asp Lys 
        435                 440                 445             


Leu Asp Ala Leu Ile Ala Phe Arg Val Pro Tyr Tyr Val Gly Pro Met 
    450                 455                 460                 


Ile Asp Pro Asn Gln Ser Asp Asp Pro Gln Asn Thr Lys Phe Ala Trp 
465                 470                 475                 480 


Met Val Arg Lys Glu Ala Gly Glu Ile Thr Pro Trp Asn Phe Glu Glu 
                485                 490                 495     


Lys Val Asp Lys Thr Ser Ser Ala Thr Asn Phe Ile Lys Arg Met Thr 
            500                 505                 510         


Thr Thr Asp Thr Tyr Leu Leu Gly Glu Pro Val Leu Pro Lys Asn Ser 
        515                 520                 525             


Leu Leu Tyr Gln Gln Phe Thr Val Leu Asn Glu Leu Asn Lys Leu Lys 
    530                 535                 540                 


Val Asn Gly Lys Ala Leu Thr Val Asp Gln Lys Lys Tyr Leu Tyr Asp 
545                 550                 555                 560 


His Val Cys Lys Lys Gln Arg Arg Val Thr Leu Lys Lys Leu Ser Asp 
                565                 570                 575     


Cys Leu Val Ala Arg Gly Asp Phe Pro Met Gly Ala Ile Phe Thr Gly 
            580                 585                 590         


Thr Thr Asp Gln Lys Asn Met Asn Asn Thr Leu Lys Thr Tyr Gln Asp 
        595                 600                 605             


Phe Gln Glu Ile Phe Gly Asp Glu Ile Asp Asn Pro Asp Arg Gln Val 
    610                 615                 620                 


Asp Phe Glu Lys Met Ile Glu Trp Ser Thr Ile Phe Glu Asp Arg Glu 
625                 630                 635                 640 


Ile Phe Lys Leu Lys Leu Ala Glu Leu Asn Trp Leu Thr Ser Glu Gln 
                645                 650                 655     


Leu Glu Arg Val Ser Lys Lys Arg Tyr Gln Gly Trp Gly Arg Leu Ser 
            660                 665                 670         


Gln Lys Leu Leu Thr His Ile Val Asn Glu Asn Gly Glu Arg Ile Ile 
        675                 680                 685             


Asp Gln Leu Trp Asn Thr Asn Gln Asn Phe Met Glu Ile Met Gly Gln 
    690                 695                 700                 


Asp Ser Val Lys Gln Ala Ile Gln Glu His Asn Gly Ala Asn Val Ala 
705                 710                 715                 720 


Gln Thr Gly Ile Asn Glu Thr Ile Asp Glu Leu Tyr Thr Ser Pro Gln 
                725                 730                 735     


Asn Lys Lys Ala Ile Arg Gln Ile Leu Leu Val Val Asp Asp Ile Lys 
            740                 745                 750         


Lys Ala Val Gly Tyr Ala Pro Ala Asn Ile Met Met Glu Phe Ala Arg 
        755                 760                 765             


Glu Asp Arg Asp Asp His Arg Leu Ile Ala Ser Arg Ala Arg Gln Leu 
    770                 775                 780                 


Glu Lys Thr Tyr Gln Glu Ile Ser Lys Asn Trp Phe Asp Asn Glu Ser 
785                 790                 795                 800 


Val Thr Gln Glu Leu Lys Asp Gln Ile Lys Asn Lys Thr Lys Phe Thr 
                805                 810                 815     


Asp Arg Leu Tyr Leu Tyr Phe Ile Gln Gly Gly Ile Asp Leu Tyr Thr 
            820                 825                 830         


Gly His Pro Leu Asn Ile Asp Gln Leu Ser Ser Tyr Asp Ile Asp His 
        835                 840                 845             


Ile Leu Pro Gln Ser Phe Leu Leu Asp Asn Ser Leu Asn Asn Arg Val 
    850                 855                 860                 


Leu Thr Ser Gln Ala Glu Asn Arg Val His Lys Glu Asp Arg Leu Pro 
865                 870                 875                 880 


Gly Glu Thr Val Ala Asn Lys Met Arg Pro Thr Trp Lys Met Met Arg 
                885                 890                 895     


Glu Gln Gly Leu Met Thr Gln Arg Lys Tyr Asn Asn Leu Thr Leu Lys 
            900                 905                 910         


Pro Asp Gln Ile Ser Lys Phe Asn Asn Arg Gly Gln Phe Ile Asn Arg 
        915                 920                 925             


Gln Leu Val Glu Thr Arg Gln Val Ile Lys Leu Ala Ala Asp Ile Met 
    930                 935                 940                 


Ser Gln Lys Tyr Gln Ala Asp Gln Thr Lys Val Val Thr Val Lys Ala 
945                 950                 955                 960 


Asn Leu Thr His His Met Arg Glu Lys Phe Asn Phe Tyr Lys Ser Arg 
                965                 970                 975     


Asn Val Asn Asp Tyr His His Ala Phe Asp Ala Tyr Leu Thr Leu Phe 
            980                 985                 990         


Val Gly Asn Trp Leu Leu Lys Glu  Tyr Pro Lys Leu Gln  Pro Tyr Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Phe Ala Lys  Thr Gly Thr Asn Asn  Leu Lys Ser 
    1010                 1015                 1020             


Phe Asn  Phe Leu Tyr Arg Phe  Glu Arg Asp His Lys  Leu Asn Asp 
    1025                 1030                 1035             


Lys Gly  Ala Ile Asp Thr Asp  Lys Asp Gln Gln Leu  Gly Tyr Met 
    1040                 1045                 1050             


Lys Arg  Ile Tyr Arg Phe Lys  Lys Met Leu Val Thr  Lys Gln Leu 
    1055                 1060                 1065             


Glu Thr  Asn Glu Gly Ala Leu  Tyr Gly Gln Thr Leu  Tyr Pro Ala 
    1070                 1075                 1080             


Pro Lys  Asn Asp Lys Lys Thr  Arg Asn Leu Ile Asn  Ser Lys Lys 
    1085                 1090                 1095             


Asn Arg  Pro Ser Asp Ile Tyr  Gly Gly Tyr Ser Asn  Glu Asn Gly 
    1100                 1105                 1110             


Ser Phe  Leu Thr Leu Val Lys  Leu Lys Lys Gly Ser  Lys Tyr Asp 
    1115                 1120                 1125             


Tyr Arg  Leu Val Ala Val Pro  Val Ser Lys Ile Ser  Glu Ile Lys 
    1130                 1135                 1140             


Glu Ser  Glu Leu Ala Thr Ile  Glu Lys Ala Ile Ala  Pro Gln Phe 
    1145                 1150                 1155             


Leu Lys  Ser Ser Gly Lys Tyr  Arg Asp Phe Gln Val  Val Leu Ser 
    1160                 1165                 1170             


Lys Ile  Leu Ile Asn Gln Lys  Val Ile Asp Asp Gly  Met Ile Phe 
    1175                 1180                 1185             


Thr Ile  Gly Ser Lys Ser Tyr  Lys His Asn Ala Lys  Gln Val Val 
    1190                 1195                 1200             


Leu Ser  Asp Asn Ser Ile Gln  Ile Val Ser Thr Leu  Ser Arg Ile 
    1205                 1210                 1215             


Ser Asp  Lys Glu Lys Leu Ile  Thr Val Phe Asp Glu  Ile Val Asp 
    1220                 1225                 1230             


Ile Thr  Ser Lys Tyr Leu Pro  Trp Tyr Lys Val Asn  Asp Val Glu 
    1235                 1240                 1245             


Lys Thr  Lys Met Leu Phe Arg  Gln Met Gln Asp Ile  Asp Glu Met 
    1250                 1255                 1260             


Lys Lys  Ile Ile Asp Asn Ser  Leu Ile Gly Leu His  Cys Asn Ala 
    1265                 1270                 1275             


Ser Ile  Thr Asp Leu Gly Lys  Val Gly Leu Ser Lys  Tyr Trp Gly 
    1280                 1285                 1290             


Arg Phe  Val Leu Thr Ala Gly  Ile Ser Leu Thr Val  Asp Ala Gln 
    1295                 1300                 1305             


Leu Ile  Tyr Gln Ser Pro Thr  Gly Leu Phe Glu His  Arg Val Lys 
    1310                 1315                 1320             


Val Ser  Asp Leu 
    1325         


<210>  828
<211>  1329
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  828

Met Asn Lys Gln Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Val Gly Trp Ser Ala Thr Glu Leu Asp Asn Arg Leu Leu His Ile Lys 
            20                  25                  30          


Gly His Asn Gly Ile Gly Val Arg Leu Phe Lys Glu Gly Glu Ser Ala 
        35                  40                  45              


Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Arg Leu Leu Asn Glu Ile Phe Ala Thr Glu Ile Ala 
65                  70                  75                  80  


Lys Val Asp Pro Ser Phe Phe Ala Arg Leu Lys Gln Ser Asn Val Ser 
                85                  90                  95      


Pro Lys Asp Pro Asn Lys Thr Met Phe Gly Asn Ile Leu Phe Asp Asp 
            100                 105                 110         


Glu Asn Leu Asp Asp Gln Lys Phe His His Asp Tyr Lys Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Gln Ala Ile Ile Asn His Pro Glu Gln Lys Phe Asp Ile 
    130                 135                 140                 


Arg Leu Ile Tyr Leu Ala Met His His Ile Ile Lys Tyr Arg Gly His 
145                 150                 155                 160 


Phe Leu Asn Gln Ala Asn Val Gln Asp Phe Lys Gly Gly Lys Ile Asp 
                165                 170                 175     


Leu Glu Lys Ser Phe Lys Ala Leu Asn Asp Ile Phe Glu Asn Gln Gly 
            180                 185                 190         


Arg Asp Leu Arg Leu Val Thr Asp Glu Ala Asn Gln Tyr Val Lys Val 
        195                 200                 205             


Leu Ser Asp Asn Ala Lys Thr Arg Ser Asp Arg Lys Lys Glu Leu Ser 
    210                 215                 220                 


Lys Leu Leu Tyr Val Pro Ile Asp Lys Asp Thr Asp Lys Asn Ser Lys 
225                 230                 235                 240 


Lys Val Thr Gly Glu Ile Leu Lys Ala Ile Leu Gly Asn Lys Ala Lys 
                245                 250                 255     


Phe Asp Val Ile Phe Gly Ile Glu Val Glu Asn Pro Lys Glu Trp Val 
            260                 265                 270         


Leu Thr Phe Asn Ser Asp Asp Phe Asp Asp Lys Ile Glu Gly Leu Ser 
        275                 280                 285             


Ser Gln Met Thr Asp Glu Asp Ser Glu Ile Leu Leu Ile Leu Lys Glu 
    290                 295                 300                 


Leu Tyr Phe Ala Leu Asn Leu Ser Asp Ile Leu Asn Asn Ser Val Thr 
305                 310                 315                 320 


Asp Lys Asn Ala Lys Thr Leu Ser Glu Ala Met Ile Asn Arg Tyr Asp 
                325                 330                 335     


Asp His Lys Asn Gln Leu Ala Met Leu Lys Lys Val Ile Asp Ser Ser 
            340                 345                 350         


Asp Lys Lys His Ala Lys Glu Leu Lys Glu Ala Tyr Ser Ala Tyr Ile 
        355                 360                 365             


Asp Gly Glu Asn Asn Lys Lys Ile Ser Phe Glu Asp Leu Lys Lys Arg 
    370                 375                 380                 


Ile Gln Lys Asn Leu Val Asp Ser Glu Leu Ser Gln Lys Ile Asn Asp 
385                 390                 395                 400 


Leu Leu Asp His Asp Tyr Phe Leu Pro Lys Gln Arg Ser Lys Glu Asn 
                405                 410                 415     


Gly Ala Ile Pro His Gln Leu Gln Gln Gln Glu Leu Asp Gln Ile Ile 
            420                 425                 430         


Glu Ser Gln Lys Gln Tyr Tyr Pro Phe Leu Ala Glu Leu Asn Pro Asn 
        435                 440                 445             


Glu Lys Arg Ser Arg Gln Ala Lys Tyr Lys Leu Asp Glu Leu Val Ala 
    450                 455                 460                 


Phe Arg Val Ser Tyr Tyr Val Gly Pro Met Val Glu Val Thr Asp Ser 
465                 470                 475                 480 


Asn Gln Glu Asn Asn Gly Lys Phe Ala Trp Met Ile Arg Lys Glu Gln 
                485                 490                 495     


Gly Glu Ile Thr Pro Trp Asn Phe Asp Glu Lys Val Asp Arg Thr Glu 
            500                 505                 510         


Thr Ala Thr Lys Phe Ile Thr Arg Met Thr Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Pro Val Leu Pro Lys Asn Ser Leu Leu Tyr Gln Lys Phe 
    530                 535                 540                 


Val Val Leu Asn Glu Leu Asn Asn Leu Lys Ile Asn Gln Glu Thr Ile 
545                 550                 555                 560 


Thr Pro Gly Gln Lys Gln Tyr Leu Tyr Glu His Val Met Lys Lys Asn 
                565                 570                 575     


Lys Arg Val Thr Leu Lys Lys Ile Ala Asp Ala Leu Val Ile Lys Gly 
            580                 585                 590         


Asp Tyr Pro Tyr Ala Pro Glu Val Lys Gly Thr Thr Asp Gln Lys Asn 
        595                 600                 605             


Leu Asn Asn Gly Leu Thr Thr Tyr Ile Asp Leu Ala Lys Ile Phe Gly 
    610                 615                 620                 


Asn Glu Leu Asp Asp Val Asp Arg Glu Ala Asp Phe Glu Lys Ile Ile 
625                 630                 635                 640 


Glu Trp Ser Thr Ile Phe Glu Asp Gln His Ile Phe Val Leu Lys Leu 
                645                 650                 655     


Gln Glu Leu Lys Trp Leu Thr Glu Lys Gln Arg Asn Gln Val Val Lys 
            660                 665                 670         


Leu Arg Tyr Gln Gly Trp Gly Lys Leu Ser Lys Glu Leu Leu Ala Gly 
        675                 680                 685             


Leu Val Asp Asp Asn Gly Gln Arg Ile Ile Asp Leu Leu Trp Ser Asp 
    690                 695                 700                 


Asn Gln Asn Phe Met Gln Ile Val Asn Gln Lys Ser Phe Lys Glu Ser 
705                 710                 715                 720 


Ile Ala Asp His Asn Gly Ala His Leu Glu Glu Lys Asn Leu Lys Asp 
                725                 730                 735     


Val Ile Asn Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Val Ile Leu Val Val Asp Asp Ile Ile Asn Ala Val Gly Tyr Glu 
        755                 760                 765             


Pro Ala Asn Ile Met Met Glu Phe Ala Arg Glu Asp Ser Asn Asp His 
    770                 775                 780                 


Arg Leu Thr Asn Ser Arg Ser Arg Gln Leu Glu Asn Val Tyr Lys Asp 
785                 790                 795                 800 


Ile Thr Asn Ser Trp Phe Asp Asn Asp Ser Val Lys Asp Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Lys Asn Lys Asp Lys Phe Thr Asp Arg Leu Tyr Leu Tyr 
            820                 825                 830         


Phe Thr Gln Gly Gly Lys Asp Leu Tyr Thr Gly Glu Pro Leu Asn Ile 
        835                 840                 845             


Asp Asp Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe 
    850                 855                 860                 


Ile Leu Asp Asn Ser Leu Asp Asn Arg Val Leu Thr Ser Gln Arg Asn 
865                 870                 875                 880 


Asn Arg Val Ser Lys Glu Asp Gln Leu Pro Gly Glu Leu Phe Gly Thr 
                885                 890                 895     


Lys Met His Ala Phe Trp Lys Arg Leu Lys Ala Ala His Leu Ile Ser 
            900                 905                 910         


Asn Lys Lys Tyr Tyr Asn Leu Thr Leu Thr Pro Asn Ser Ile Ser Lys 
        915                 920                 925             


Phe Asn Asn Gln Gly Arg Phe Ile Asn Arg Gln Leu Val Glu Thr Arg 
    930                 935                 940                 


Gln Val Ile Lys Leu Ala Ala Glu Ile Leu His Asn Arg Tyr Ser Glu 
945                 950                 955                 960 


Glu Lys Gly Thr Asn Ile Val Thr Ile Lys Ala Asn Leu Thr His His 
                965                 970                 975     


Met Arg Glu Lys Phe Asn Phe Tyr Lys Asn Arg Asn Val Asn Asp Tyr 
            980                 985                 990         


His His Ala Phe Asp Ala Tyr Leu  Thr Ala Phe Val Gly  Asn Trp Leu 
        995                 1000                 1005             


Leu Gln  Gln Tyr Pro Lys Leu  Lys Pro Tyr Phe Val  Tyr Gly Asp 
    1010                 1015                 1020             


Phe Ala  Lys Thr Glu Ile Asn  Asn Leu Lys Ser Phe  Asn Met Leu 
    1025                 1030                 1035             


Tyr Lys  Phe Glu Gln Ala His  Gln Leu Asn Glu Lys  Asp Gly Lys 
    1040                 1045                 1050             


Ile Ala  Lys Asp Thr Asp Ala  Leu Leu Gly Tyr Met  Lys Asn Val 
    1055                 1060                 1065             


Tyr His  Phe Lys Lys Met Leu  Val Thr Lys Glu Leu  Glu Thr Asn 
    1070                 1075                 1080             


His Gly  Ala Leu Phe Asn Gln  Thr Leu Tyr Pro Ser  Pro Ala His 
    1085                 1090                 1095             


Asp Ile  Lys Lys Arg Gln Leu  Ile Lys Pro Lys Asp  Asn Arg Pro 
    1100                 1105                 1110             


Thr Asp  Ile Tyr Gly Gly Tyr  Thr His Glu Glu Arg  Lys Tyr Met 
    1115                 1120                 1125             


Ser Leu  Val Lys Val Ile Gly  Lys Lys Asp Asn Glu  Tyr Arg Met 
    1130                 1135                 1140             


Ala Asn  Val Pro Leu Leu Lys  Ile Asn Lys Asn Asn  Asp Ile Lys 
    1145                 1150                 1155             


Glu Ile  Leu Lys Ser Gln Phe  Ser Asn Lys Lys Phe  Glu Ile Val 
    1160                 1165                 1170             


Leu Pro  Lys Val Leu Lys Asn  Gln Leu Phe Ser Glu  Asn Gly Ala 
    1175                 1180                 1185             


Leu Phe  Val Ile Gly Ser Lys  Gly Tyr Lys Tyr Asn  Ala Asn Gln 
    1190                 1195                 1200             


Leu Val  Leu Ser Asp Leu Asp  Met Lys Leu Leu Asn  Asp Leu Ser 
    1205                 1210                 1215             


Asn Asn  Lys Ser Ile Asp Glu  Val Thr Ile Asn Gln  Leu Phe Lys 
    1220                 1225                 1230             


Asn Ile  Val Asp Ser Thr Asn  Lys Tyr Met Pro Leu  Tyr Glu Ile 
    1235                 1240                 1245             


Lys Asn  Leu Asn Glu Val Val  Glu Lys Phe Asn Asn  Leu Glu Phe 
    1250                 1255                 1260             


Asn Asp  Lys Lys Lys Thr Ile  Thr Asp Phe Leu Leu  Ala Thr His 
    1265                 1270                 1275             


Ala Asn  Pro Thr Met Ser Asn  Leu Lys Asn Ile Gly  Leu Pro Tyr 
    1280                 1285                 1290             


Phe Gly  Arg Phe Ile Arg Asn  Thr Gly Ile Lys Leu  Ser Glu Asn 
    1295                 1300                 1305             


Ala Asn  Val Ile Tyr Gln Ser  Pro Thr Gly Leu Phe  Ser Arg Lys 
    1310                 1315                 1320             


Ile Lys  Ile Ser Asp Phe 
    1325                 


<210>  829
<211>  1330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  829

Met Lys Asn Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Tyr Ser Val Val Thr Asp Asp Tyr Lys Val Ile Ser Lys Lys Met 
            20                  25                  30          


Asn Val Phe Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Glu Ser Gly Gln Thr Ala Gln Glu Ala Arg Met 
    50                  55                  60                  


Lys Arg Thr Ser Arg Arg Arg Ile Ala Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Gln Pro Glu Met Asn His Leu Asp Asn Asn 
                85                  90                  95      


Phe Phe Tyr Arg Leu Asn Glu Ser Phe Leu Val Ala Asp Asp Ala Lys 
            100                 105                 110         


Tyr Asp Lys His Pro Ile Phe Gly Thr Leu Asp Glu Glu Ile His Phe 
        115                 120                 125             


His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 


Gly Asp Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Ile Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly Asn Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     


Glu Asn Asn Ser Val Ile Glu Leu Ser Lys Val Phe Val Gln Leu Tyr 
            180                 185                 190         


Asn Gln Thr Leu Ser Glu Leu Glu Gly Phe Gln Phe Ile Asp Glu Ser 
        195                 200                 205             


Ile Asp Phe Ser Glu Val Leu Thr Gln Gln Leu Ser Lys Ser Glu Arg 
    210                 215                 220                 


Ala Asp Asn Val Leu Lys Leu Phe Pro Asp Glu Lys Gly Thr Gly Ile 
225                 230                 235                 240 


Phe Ala Gln Phe Ile Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys 
                245                 250                 255     


Lys Val Phe Gln Leu Glu Glu Asp Gln Lys Leu Gln Leu Ser Thr Asp 
            260                 265                 270         


Asp Tyr Glu Glu Asn Ile Glu Asn Leu Leu Ala Ile Ile Gly Asp Glu 
        275                 280                 285             


Tyr Gly Asp Ile Phe Val Ala Ala Gln Asn Leu Tyr Gln Ala Ile Leu 
    290                 295                 300                 


Leu Ala Gly Ile Leu Thr Ser Thr Glu Lys Thr Arg Ala Lys Leu Ser 
305                 310                 315                 320 


Ala Ser Met Ile Gln Arg Tyr Glu Glu His Ala Lys Asp Leu Lys Leu 
                325                 330                 335     


Leu Lys Arg Phe Val Lys Glu His Ile Pro Asp Lys Tyr Ala Glu Ile 
            340                 345                 350         


Phe Asn Asp Ala Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys 
        355                 360                 365             


Thr Lys Glu Glu Glu Phe Tyr Lys Tyr Leu Lys Thr Thr Leu Val Gln 
    370                 375                 380                 


Lys Ser Gly Tyr Gln Tyr Phe Ile Glu Lys Ile Glu Gln Glu Asn Phe 
385                 390                 395                 400 


Leu Arg Lys Gln Arg Ile Tyr Asp Asn Gly Val Ile Pro His Gln Val 
                405                 410                 415     


His Ala Glu Glu Leu Arg Ala Ile Leu Arg Lys Gln Glu Lys Tyr Tyr 
            420                 425                 430         


Ser Phe Leu Lys Glu Asn His Glu Lys Ile Glu Gln Ile Phe Lys Val 
        435                 440                 445             


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Lys His Asn Glu Gln Ser 
    450                 455                 460                 


Arg Phe Ala Trp Asn Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp 
465                 470                 475                 480 


Asn Met Asn Asp Val Val Asp Glu Asn Ala Ser Ala Val Ala Phe Ile 
                485                 490                 495     


Glu Arg Met Thr Ile Lys Asp Ile Tyr Leu Asn Glu Asn Val Leu Pro 
            500                 505                 510         


Arg His Ser Leu Ile Tyr Glu Lys Phe Thr Val Phe Asn Glu Leu Thr 
        515                 520                 525             


Lys Val Leu Tyr Ala Asp Asp Arg Gly Val Phe Gln Arg Phe Ser Ala 
    530                 535                 540                 


Glu Glu Lys Glu Asp Ile Phe Glu Lys Leu Phe Lys Ser Glu Arg Lys 
545                 550                 555                 560 


Val Thr Lys Lys Lys Leu Glu Asn Tyr Leu Arg Ile Glu Leu Ser Ile 
                565                 570                 575     


Ser Ser Pro Ser Val Lys Gly Ile Glu Glu Gln Phe Asn Ala Asn Phe 
            580                 585                 590         


Gly Thr Tyr Leu Asp Leu Lys Lys Phe Asp Glu Leu His Pro Tyr Leu 
        595                 600                 605             


Asp Asp Glu Lys Tyr Gln Asp Thr Leu Glu Glu Val Ile Lys Val Leu 
    610                 615                 620                 


Thr Val Phe Glu Asp Arg Ser Met Ile Gln Asn Gln Leu Glu Gln Leu 
625                 630                 635                 640 


Pro Leu Asn Leu Ser Thr Lys Thr Ile Lys Ala Leu Ser Arg Arg Lys 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Ala Arg Leu Ile Asp Gly Ile His 
            660                 665                 670         


Asp Lys Asn Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Glu Asp Glu 
        675                 680                 685             


Ser Asp Ser Tyr Ile Val Asn Arg Asn Phe Met Gln Leu Ile Asn Asp 
    690                 695                 700                 


Asp His Leu Ser Phe Lys Lys Ile Ile Glu Asp Ser Gln Pro Tyr Lys 
705                 710                 715                 720 


Glu Gln Gln Ser Ala Glu Glu Ile Val Ser Glu Leu Ser Gly Ser Pro 
                725                 730                 735     


Ala Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu 
            740                 745                 750         


Val Ala Ile Met Gly Tyr Lys Pro Lys Asn Ile Val Val Glu Met Ala 
        755                 760                 765             


Arg Glu Asn Gln Thr Thr Gly Arg Gly Lys Gln Asn Ser Lys Pro Arg 
    770                 775                 780                 


Leu Lys Gly Ile Glu Asn Gly Leu Lys Glu Phe Ser Asp Ser Val Leu 
785                 790                 795                 800 


Lys Gly Ser Ser Ile Asp Asn Lys Gln Leu Gln Asn Asp Arg Leu Tyr 
                805                 810                 815     


Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly His Glu Leu 
            820                 825                 830         


Asp Ile Asp His Leu Ser Thr Tyr Asp Ile Asp His Ile Ile Pro Gln 
        835                 840                 845             


Ser Phe Leu Thr Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Thr Ser 
    850                 855                 860                 


Lys Ser Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val 
865                 870                 875                 880 


Arg Lys Met Asp Arg Phe Trp Arg Lys Leu Leu Asn Ala Lys Leu Ile 
                885                 890                 895     


Ser Glu Arg Lys Tyr Thr Asn Leu Thr Lys Lys Glu Leu Thr Glu Ser 
            900                 905                 910         


Asp Lys Ala Gly Phe Leu Lys Arg Gln Leu Val Glu Thr Arg Gln Ile 
        915                 920                 925             


Thr Lys His Val Ala Thr Ile Leu Asp Ser Lys Phe Asn Glu Asp Ser 
    930                 935                 940                 


Asn Asn Arg Asp Val Gln Ile Ile Thr Leu Lys Ser Ala Leu Val Ser 
945                 950                 955                 960 


Glu Phe Arg Lys Thr Phe Asn Leu Tyr Lys Val Arg Glu Ile Asn Asp 
                965                 970                 975     


Leu His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala Leu Ser 
            980                 985                 990         


Leu Leu Arg Val Tyr Pro Gln Leu  Lys Pro Glu Phe Val  Tyr Gly Glu 
        995                 1000                 1005             


Tyr Gly  Lys Asn Ser Ile His  Asp Gln Asn Lys Ala  Thr Ile Lys 
    1010                 1015                 1020             


Lys Gln  Phe Tyr Ser Asn Ile  Thr Arg Tyr Phe Ala  Ser Lys Asp 
    1025                 1030                 1035             


Tyr Ile  Ile Asn Asp Asp Gly  Glu Ile Leu Trp Asn  Lys Gln Glu 
    1040                 1045                 1050             


Thr Ile  Ala Gln Val Ile Lys  Thr Leu Gly Met His  Gln Val Asn 
    1055                 1060                 1065             


Val Val  Lys Lys Val Glu Ile  Gln Lys Gly Gly Phe  Ser Lys Glu 
    1070                 1075                 1080             


Ser Ile  Gln Pro Lys Gly Glu  Ser Gln Lys Leu Ile  Arg Arg Lys 
    1085                 1090                 1095             


Gln Gln  Trp Asn Thr Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Val 
    1100                 1105                 1110             


Val Ala  Tyr Ala Ile Leu Leu  Ser Phe Asp Lys Gly  Lys Arg Lys 
    1115                 1120                 1125             


Ala Arg  Ser Phe Lys Ile Val  Gly Ile Thr Ile Gln  Asp Arg Glu 
    1130                 1135                 1140             


Ser Phe  Glu Gly Asn Pro Ile  Leu Tyr Leu Ser Lys  Lys Asp Tyr 
    1145                 1150                 1155             


His Asn  Pro Lys Val Glu Ala  Ile Leu Pro Lys Tyr  Ser Leu Phe 
    1160                 1165                 1170             


Glu Phe  Glu Asn Gly Arg Arg  Arg Met Val Ala Ser  Ala Ser Glu 
    1175                 1180                 1185             


Thr Gln  Lys Gly Asn Gln Leu  Ile Ile Pro Gly His  Leu Met Glu 
    1190                 1195                 1200             


Leu Leu  Tyr His Ser Lys Lys  Ile Ile Asn Gly Lys  Asn Ser Asp 
    1205                 1210                 1215             


Ser Val  Ser Tyr Ile Gln Asn  Asn Lys Glu Lys Phe  Arg Glu Ile 
    1220                 1225                 1230             


Phe Glu  Tyr Ile Val Asp Phe  Ser Ser Lys Tyr Ile  Ser Ala Asp 
    1235                 1240                 1245             


Ala Asn  Leu Asn Lys Ile Glu  Lys Ile Phe Glu Asn  Asn Phe His 
    1250                 1255                 1260             


Lys Ala  Ser Glu Gln Glu Ile  Ala Lys Ser Phe Ile  Asn Leu Leu 
    1265                 1270                 1275             


Thr Phe  Thr Ala Met Gly Ala  Pro Ala Asp Phe Glu  Phe Phe Gly 
    1280                 1285                 1290             


Glu Lys  Ile Pro Arg Lys Arg  Tyr Val Ser Ile Ser  Glu Ile Ile 
    1295                 1300                 1305             


Asp Ala  Val Phe Ile His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr 
    1310                 1315                 1320             


Arg Val  Arg Leu Thr Glu Val  
    1325                 1330 


<210>  830
<211>  1330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  830

Met Glu Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Val Ala Arg Lys Met 
            20                  25                  30          


Ser Ile Gln Gly Asp Ser Asn Arg Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Ala Arg Leu Phe Glu Glu Gly Lys Thr Ala Gln Phe Arg Arg Ile 
    50                  55                  60                  


Lys Arg Thr Asn Arg Arg Arg Ile Ala Arg Arg Arg Gln Arg Val Leu 
65                  70                  75                  80  


Ala Leu Gln Asp Ile Phe Ala Glu Glu Ile His Lys Lys Asp Pro Asn 
                85                  90                  95      


Phe Phe Ala Arg Leu Glu Glu Gly Asp Arg Val Glu Ala Asp Lys Arg 
            100                 105                 110         


Phe Ala Lys Phe Pro Val Phe Ala Thr Leu Ser Glu Glu Lys Asn Tyr 
        115                 120                 125             


His Arg Gln Tyr Pro Thr Ile Tyr His Leu Arg His Asp Leu Ala Asn 
    130                 135                 140                 


Ser Lys Glu Gln Ala Asp Ile Arg Leu Val Tyr Leu Ala Ile Ala His 
145                 150                 155                 160 


Cys Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     


Glu Asn Thr Ser Val Thr Glu Asn Tyr Gln Gln Phe Leu Gln Ala Tyr 
            180                 185                 190         


Gln Gln Phe Phe Pro Glu Pro Ile Gly Asp Leu Asp Asp Ala Val Pro 
        195                 200                 205             


Ile Leu Thr Glu Arg Leu Ser Lys Ala Lys Arg Val Glu Lys Val Leu 
    210                 215                 220                 


Ala Tyr Tyr Pro Ser Glu Lys Ser Thr Gly Asn Phe Ala Gln Phe Leu 
225                 230                 235                 240 


Lys Leu Met Val Gly Asn Gln Ala Asn Phe Lys Lys Thr Phe Asp Leu 
                245                 250                 255     


Glu Glu Glu Met Lys Leu Asn Phe Thr Arg Asp Cys Tyr Glu Glu Asp 
            260                 265                 270         


Leu Asn Glu Leu Leu Glu Lys Thr Ser Asp Asp Tyr Ala Glu Leu Phe 
        275                 280                 285             


Leu Lys Ala Lys Gly Val Tyr Asp Ala Ile Leu Leu Ser Gln Ile Leu 
    290                 295                 300                 


Ser Lys Ser Asp Asp Glu Thr Lys Ala Lys Leu Ser Ala Asn Met Lys 
305                 310                 315                 320 


Leu Arg Phe Glu Glu His Gln Arg Asp Leu Lys Gln Leu Lys Glu Leu 
                325                 330                 335     


Val Arg Arg Asp Leu Pro Lys Lys Tyr Asp Asp Phe Phe Lys Asn Arg 
            340                 345                 350         


Ser Lys Asn Gly Tyr Ala Gly Tyr Val Lys Gly Lys Ala Thr Gln Glu 
        355                 360                 365             


Asp Phe Tyr Lys Phe Leu Arg Thr Glu Leu Ala Gly Leu Glu Glu Ser 
    370                 375                 380                 


Gln Ser Ile Met Glu Lys Ile Asp Leu Glu Ile Tyr Leu Leu Lys Gln 
385                 390                 395                 400 


Arg Thr Phe Ala Asn Gly Val Ile Pro His Gln Ile His Leu Val Glu 
                405                 410                 415     


Met Arg Glu Ile Met Asp Arg Gln Lys Arg Phe Tyr Pro Phe Leu Lys 
            420                 425                 430         


Gly Ala Gln Gly Lys Ile Glu Lys Leu Leu Thr Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Leu Ala Gln Glu Gly Gln Ser Pro Phe Ala Trp Ile 
    450                 455                 460                 


Lys Arg Lys Ser Pro Ser Gln Ile Thr Pro Trp Asn Phe Ala Glu Val 
465                 470                 475                 480 


Val Asp Lys Glu Asn Ser Ala Ile Glu Phe Ile Glu Arg Met Thr Asn 
                485                 490                 495     


Gln Asp Thr Tyr Leu Pro Lys Glu Lys Val Leu Pro Lys Gln Ser Leu 
            500                 505                 510         


Ile Tyr Gln Arg Phe Met Ile Phe Asn Glu Leu Thr Lys Val Ser Tyr 
        515                 520                 525             


Thr Asp Glu Arg Gly Lys Ser His Tyr Phe Ser Ser Glu Gln Lys Arg 
    530                 535                 540                 


Lys Ile Phe Asn Glu Leu Phe Lys Gln His Pro Arg Val Thr Glu Lys 
545                 550                 555                 560 


Gln Leu Arg Lys Phe Leu Glu Leu Asn Glu Gln Ile Asp Ser Thr Glu 
                565                 570                 575     


Ile Lys Gly Ile Glu Thr Ser Phe Asn Ala Ser Tyr Ser Thr Tyr His 
            580                 585                 590         


Asp Leu Leu Lys Leu Ser Asp Gln Met Asp Thr Leu Leu Asp Asp Pro 
        595                 600                 605             


Asp Met Thr Thr Met Phe Glu Glu Ile Ile Lys Ile Leu Thr Ile Phe 
    610                 615                 620                 


Glu Asp Arg Glu Met Ile Arg Glu Gln Leu Lys Pro Tyr Glu Thr Val 
625                 630                 635                 640 


Leu Gly Leu Pro Ala Ile Lys Lys Leu Ala Lys Lys His Tyr Thr Gly 
                645                 650                 655     


Trp Gly Arg Leu Ser Glu Lys Met Ile Gln Gly Met Arg Glu Lys Gln 
            660                 665                 670         


Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Asp Phe Pro 
        675                 680                 685             


Cys Asn Arg Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp His Leu 
    690                 695                 700                 


Ser Phe Lys Glu Thr Ile Ala Asn Glu Leu Ile Met Ser Asp Ser Asn 
705                 710                 715                 720 


Val Leu Leu Asp Gln Val Lys Ala Ile Pro Gly Ser Pro Ala Val Lys 
                725                 730                 735     


Lys Gly Ile Trp Gln Ser Ile Lys Ile Val Glu Glu Ile Ile Gly Ile 
            740                 745                 750         


Ile Gly Lys Ala Pro Lys Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Arg Thr Ser Arg Ser Arg Pro Arg Leu Lys Ala Leu Glu Glu Ala 
    770                 775                 780                 


Leu Lys Asn Ile Asp Ser Pro Leu Leu Lys Asp Tyr Pro Thr Asp Asn 
785                 790                 795                 800 


Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly 
                805                 810                 815     


Lys Asp Met Tyr Thr Gly Glu Pro Leu Glu Ile His Arg Leu Ser Glu 
            820                 825                 830         


Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Val Asp Asn Ser 
        835                 840                 845             


Leu Asp Asn Lys Val Leu Val Ser Ser Lys Val Asn Arg Gly Lys Leu 
    850                 855                 860                 


Asp Asn Ala Pro Asp Pro Leu Val Val Lys Arg Met Arg Ser His Trp 
865                 870                 875                 880 


Glu Lys Leu His Gln Ala Lys Leu Ile Ser Asp Lys Lys Leu Ala Asn 
                885                 890                 895     


Leu Thr Lys Gln Asn Leu Thr Glu Ala Asp Lys Ala Arg Phe Ile Gln 
            900                 905                 910         


Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Asn Leu 
        915                 920                 925             


Leu His Gln His Phe Asn Leu Pro Glu Glu Val Ser Ala Thr Glu Lys 
    930                 935                 940                 


Thr Ser Ile Ile Thr Leu Lys Ser Thr Leu Thr Ser Gln Phe Arg Gln 
945                 950                 955                 960 


Met Phe Asp Ile Tyr Lys Val Arg Glu Ile Asn His His His His Ala 
                965                 970                 975     


His Asp Ala Tyr Leu Asn Gly Val Val Ala Met Thr Leu Leu Lys Lys 
            980                 985                 990         


Tyr Pro Lys Leu Ala Pro Glu Phe  Val Tyr Gly Ser Tyr  Ile Lys Gly 
        995                 1000                 1005             


Asp Ile  Asn Gln Ile Asn Lys  Ala Thr Ala Lys Lys  Glu Phe Tyr 
    1010                 1015                 1020             


Ser Asn  Ile Met Lys Phe Phe  Glu Ser Glu Glu Ile  Ile Cys Asp 
    1025                 1030                 1035             


Glu Gln  Gly Glu Val Ile Trp  Asn Lys Lys Arg Asp  Leu Ser Thr 
    1040                 1045                 1050             


Ile Lys  Lys Thr Ile Gly Ala  His Gln Val Asn Ile  Val Lys Lys 
    1055                 1060                 1065             


Val Glu  Lys Gln Lys Gly Gly  Phe Tyr Lys Glu Thr  Ile Asn Ser 
    1070                 1075                 1080             


Lys Ala  Asn Pro Glu Lys Leu  Ile Pro Arg Lys Ala  Ser Leu Asp 
    1085                 1090                 1095             


Pro Leu  Lys Tyr Gly Gly Tyr  Gly Ser Pro Leu Val  Ala Tyr Thr 
    1100                 1105                 1110             


Val Ile  Phe Ile Phe Glu Lys  Gly Lys Gln Lys Lys  Val Thr Lys 
    1115                 1120                 1125             


Gly Ile  Glu Gly Ile Thr Val  Met Glu Gln Leu Arg  Phe Glu Gln 
    1130                 1135                 1140             


Asp Pro  Arg Glu Phe Leu Lys  Thr Lys Gly Tyr Glu  Gly Val Lys 
    1145                 1150                 1155             


Gln Trp  Leu Ile Leu Pro Lys  Tyr Ile Leu Phe Glu  Ala Gln Gly 
    1160                 1165                 1170             


Gly Tyr  Arg Arg Met Ile Ala  Ser His Gln Glu Thr  Gln Lys Ala 
    1175                 1180                 1185             


Asn Ser  Leu Ile Leu Pro Glu  Asn Leu Val Thr Leu  Leu Tyr His 
    1190                 1195                 1200             


Ala Arg  His Tyr Asp Glu Ile  Asn His Lys Val Ser  Phe Asp Tyr 
    1205                 1210                 1215             


Val Asn  Ala His Lys Glu Gly  Phe Asn Asp Ile Phe  Asp Phe Ile 
    1220                 1225                 1230             


Ser Asp  Phe Gly Val Arg Tyr  Ile Leu Ala Pro Gln  His Leu Glu 
    1235                 1240                 1245             


Lys Ile  Lys Val Ala Tyr Glu  Lys Asn Lys Glu Val  Asp Leu Lys 
    1250                 1255                 1260             


Glu Met  Ile Asp Ala Ile Leu  Ser Leu Leu Lys Phe  Thr Leu Phe 
    1265                 1270                 1275             


Gly Ala  Ser Val Glu Phe Lys  Phe Phe Asp Ile Lys  Ile Leu Lys 
    1280                 1285                 1290             


Arg Tyr  Lys Ser Leu Thr Asp  Ile Trp Glu Ala Thr  Ile Ile Tyr 
    1295                 1300                 1305             


Gln Ser  Val Thr Gly Leu Tyr  Glu Arg Arg Val Glu  Val Arg Lys 
    1310                 1315                 1320             


Leu Trp  Asp Gly Glu Arg Leu  
    1325                 1330 


<210>  831
<211>  1331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  831

Met Asn Lys Gln Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Val Gly Trp Ser Ala Thr Glu Leu Asp Asn Arg Leu Leu His Ile Lys 
            20                  25                  30          


Gly His Asn Gly Ile Gly Val Arg Leu Phe Lys Glu Gly Glu Ser Ala 
        35                  40                  45              


Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Arg Leu Leu Asn Glu Ile Phe Ala Thr Glu Ile Ala 
65                  70                  75                  80  


Lys Val Asp Pro Ser Phe Phe Ala Arg Leu Lys Gln Ser Asn Val Ser 
                85                  90                  95      


Pro Lys Asp Pro Asn Lys Thr Met Phe Glu Asn Ile Leu Phe Asp Asp 
            100                 105                 110         


Glu Asn Leu Asp Asp Gln Lys Phe His His Asp Tyr Lys Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Gln Ala Ile Ile Asn His Pro Glu Gln Lys Phe Asp Ile 
    130                 135                 140                 


Arg Leu Ile Tyr Leu Ala Met His His Ile Ile Lys Tyr Arg Gly His 
145                 150                 155                 160 


Phe Leu Asn Gln Ala Asn Val Gln Asp Phe Lys Gly Gly Lys Ile Asp 
                165                 170                 175     


Leu Glu Lys Ser Phe Lys Ala Leu Asn Asp Ile Phe Glu Asn Gln Gly 
            180                 185                 190         


Arg Asp Leu Arg Leu Val Thr Asp Lys Ala Asn Gln Tyr Val Lys Val 
        195                 200                 205             


Leu Ser Asp Asn Ala Lys Thr Arg Ser Asp Arg Lys Lys Glu Leu Ser 
    210                 215                 220                 


Lys Leu Leu Tyr Val Pro Ile Asp Lys Asp Thr Asp Lys Asn Ser Lys 
225                 230                 235                 240 


Lys Val Thr Gly Glu Ile Leu Lys Ala Ile Leu Gly Asn Lys Ala Lys 
                245                 250                 255     


Phe Asp Val Ile Phe Gly Ile Glu Val Glu Asn Pro Lys Glu Trp Val 
            260                 265                 270         


Leu Thr Phe Asn Ser Asp Asp Phe Asp Asp Lys Ile Glu Gly Leu Ser 
        275                 280                 285             


Ser Gln Met Thr Asp Glu Asp Ser Glu Ile Leu Leu Ile Leu Lys Glu 
    290                 295                 300                 


Leu Tyr Phe Ala Leu Asn Leu Ser Asp Ile Leu Asn Asn Ser Val Thr 
305                 310                 315                 320 


Asp Lys Asn Ala Lys Thr Leu Ser Glu Ala Met Ile Asn Arg Tyr Asp 
                325                 330                 335     


Asp His Lys Asn Gln Leu Ala Met Leu Lys Lys Val Ile Asp Ser Ser 
            340                 345                 350         


Asp Lys Lys His Ala Lys Glu Leu Lys Glu Ala Tyr Ser Ala Tyr Ile 
        355                 360                 365             


Asp Gly Glu Asn Asn Lys Lys Ile Ser Phe Glu Asp Leu Lys Lys Arg 
    370                 375                 380                 


Ile Gln Lys Asn Leu Val Asp Ser Glu Leu Ser Gln Lys Ile Asn Asp 
385                 390                 395                 400 


Leu Leu Asp His Asp Tyr Phe Leu Pro Lys Gln Arg Ser Lys Glu Asn 
                405                 410                 415     


Gly Ala Ile Pro His Gln Leu Gln Gln Gln Glu Leu Asp Gln Ile Ile 
            420                 425                 430         


Glu Ser Gln Lys Gln Tyr Tyr Pro Phe Leu Ala Glu Leu Asn Pro Asn 
        435                 440                 445             


Glu Lys Arg Ser Arg Gln Ala Lys Tyr Lys Leu Asp Glu Leu Val Ala 
    450                 455                 460                 


Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Val Glu Val Thr Asp Ser 
465                 470                 475                 480 


Asn Gln Glu Asn Asn Gly Lys Phe Ala Trp Met Ile Arg Lys Glu Gln 
                485                 490                 495     


Gly Glu Ile Thr Pro Trp Asn Phe Asp Glu Lys Val Asp Arg Thr Glu 
            500                 505                 510         


Thr Ala Thr Lys Phe Ile Thr Arg Met Thr Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Pro Val Leu Pro Lys Asn Ser Leu Leu Tyr Gln Lys Phe 
    530                 535                 540                 


Val Val Leu Asn Glu Leu Asn Asn Leu Lys Ile Asn Gln Glu Ile Ile 
545                 550                 555                 560 


Thr Pro Gly Gln Lys Gln Tyr Leu Tyr Glu His Val Met Lys Lys Asn 
                565                 570                 575     


Lys Arg Val Thr Leu Lys Lys Ile Ala Asp Ala Leu Val Ile Lys Gly 
            580                 585                 590         


Asp Tyr Pro Tyr Ala Pro Glu Val Lys Gly Thr Thr Asp Gln Lys Asn 
        595                 600                 605             


Leu Asn Asn Gly Leu Thr Thr Tyr Ile Asp Leu Ala Lys Ile Phe Gly 
    610                 615                 620                 


Asn Glu Leu Asp Asp Val Asp Arg Glu Ala Asp Phe Glu Lys Ile Ile 
625                 630                 635                 640 


Glu Trp Ser Thr Ile Phe Glu Asp Gln His Ile Phe Ala Leu Lys Leu 
                645                 650                 655     


Gln Glu Leu Lys Trp Leu Thr Glu Lys Gln Arg Asn Gln Val Val Lys 
            660                 665                 670         


Leu Arg Tyr Gln Gly Trp Gly Lys Leu Ser Lys Glu Leu Leu Ala Gly 
        675                 680                 685             


Leu Val Asp Asp Asn Gly Gln Arg Ile Ile Asp Leu Leu Trp Ser Asp 
    690                 695                 700                 


Asn Gln Asn Phe Met Gln Ile Val Asn Gln Lys Ser Phe Lys Glu Ser 
705                 710                 715                 720 


Ile Ala Asp His Asn Gly Ala His Leu Glu Glu Lys Asn Leu Lys Asp 
                725                 730                 735     


Val Ile Asn Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Val Ile Leu Val Val Asp Asp Ile Ile Asn Ala Val Gly Tyr Glu 
        755                 760                 765             


Pro Ala Asn Ile Met Met Glu Phe Ala Arg Glu Asp Ser Asn Asp His 
    770                 775                 780                 


Arg Leu Thr Asn Ser Arg Ser Arg Gln Leu Glu Asn Val Tyr Lys Asp 
785                 790                 795                 800 


Ile Thr Asn Ser Trp Phe Asp Asn Asp Ser Val Lys Asp Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Lys Asn Lys Asp Lys Phe Thr Asp Arg Leu Tyr Leu Tyr 
            820                 825                 830         


Phe Thr Gln Gly Gly Lys Asp Leu Tyr Thr Gly Glu Pro Leu Asn Ile 
        835                 840                 845             


Asp Asp Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe 
    850                 855                 860                 


Ile Leu Asp Asn Ser Leu Asp Asn Arg Val Leu Thr Ser Gln Arg Asn 
865                 870                 875                 880 


Asn Arg Val Ser Lys Glu Asp Gln Leu Pro Gly Glu Leu Phe Gly Thr 
                885                 890                 895     


Lys Met His Ala Phe Trp Lys Arg Leu Lys Ala Ala His Leu Ile Ser 
            900                 905                 910         


Asn Lys Lys Tyr Tyr Asn Leu Thr Leu Thr Pro Asn Ser Ile Ser Lys 
        915                 920                 925             


Phe Asn Asn Gln Gly Arg Phe Ile Asn Arg Gln Leu Val Glu Thr Arg 
    930                 935                 940                 


Gln Val Ile Lys Leu Ala Ala Glu Ile Leu His Asn Arg Tyr Ser Glu 
945                 950                 955                 960 


Glu Lys Gly Thr Asn Ile Val Thr Ile Lys Ala Asn Leu Thr His His 
                965                 970                 975     


Met Arg Glu Lys Phe Asn Phe Tyr Lys Asn Arg Asn Val Asn Asp Tyr 
            980                 985                 990         


His His Ala Phe Asp Ala Tyr Leu  Thr Ala Phe Val Gly  Asn Trp Leu 
        995                 1000                 1005             


Leu Gln  Gln Tyr Pro Lys Leu  Lys Pro Tyr Phe Val  Tyr Gly Asp 
    1010                 1015                 1020             


Phe Ala  Lys Thr Glu Ile Asn  Asn Leu Lys Ser Phe  Asn Met Leu 
    1025                 1030                 1035             


Tyr Lys  Phe Glu Gln Ala His  Gln Leu Asn Glu Lys  Asp Gly Lys 
    1040                 1045                 1050             


Ile Ala  Lys Asp Ala Asp Ala  Leu Leu Gly Tyr Met  Lys Asn Val 
    1055                 1060                 1065             


Tyr His  Phe Lys Lys Met Leu  Val Thr Lys Lys Leu  Glu Thr Asn 
    1070                 1075                 1080             


His Gly  Ala Leu Phe Lys Gln  Thr Leu Tyr Pro Ser  Pro Ala His 
    1085                 1090                 1095             


Asp Ile  Lys Lys Arg Gln Leu  Ile Lys Pro Lys Asp  Asn Arg Pro 
    1100                 1105                 1110             


Thr Asp  Ile Tyr Gly Gly Tyr  Thr His Glu Glu Arg  Lys Tyr Met 
    1115                 1120                 1125             


Ser Leu  Val Lys Val Ile Gly  Lys Lys Asp Asn Glu  Tyr Arg Met 
    1130                 1135                 1140             


Ala Asn  Val Pro Leu Leu Lys  Ile Asn Lys Asn Asn  Asp Ile Lys 
    1145                 1150                 1155             


Glu Ile  Leu Lys Ser Gln Phe  Ser Asn Lys Lys Phe  Glu Ile Val 
    1160                 1165                 1170             


Leu Pro  Lys Val Leu Lys Asn  Gln Leu Phe Ser Glu  Asn Gly Ala 
    1175                 1180                 1185             


Leu Phe  Val Ile Gly Ser Lys  Gly Tyr Lys Tyr Asn  Ala Asn Gln 
    1190                 1195                 1200             


Leu Val  Leu Ser Asp Leu Asp  Met Lys Leu Leu Asn  Asp Leu Ser 
    1205                 1210                 1215             


Asn Asn  Lys Ser Ile Asp Glu  Val Thr Ile Asn Gln  Leu Phe Lys 
    1220                 1225                 1230             


Asn Ile  Val Asp Ser Thr Asn  Lys Tyr Met Pro Leu  Tyr Glu Ile 
    1235                 1240                 1245             


Lys Asn  Leu Asn Glu Val Val  Glu Lys Phe Asn Asn  Leu Glu Phe 
    1250                 1255                 1260             


Asn Asp  Lys Lys Lys Thr Ile  Thr Asp Phe Leu Leu  Ala Thr His 
    1265                 1270                 1275             


Ala Asn  Pro Thr Met Ser Asn  Leu Lys Asn Ile Gly  Leu Pro Ser 
    1280                 1285                 1290             


Tyr Phe  Gly Arg Phe Thr Ser  Gly Asn Ala Gly Ile  Lys Leu Ser 
    1295                 1300                 1305             


Glu Asn  Ala Asn Val Ile Tyr  Gln Ser Pro Thr Gly  Leu Phe Ser 
    1310                 1315                 1320             


Arg Lys  Val Lys Ile Ser Asp  Phe 
    1325                 1330     


<210>  832
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  832

Met Lys Asn Arg Glu Asn Ile Lys Tyr Tyr Ile Gly Val Asp Ile Gly 
1               5                   10                  15      


Thr Asn Ser Val Gly Trp Ala Val Ile Asp Glu Asn Gly Asn Leu Leu 
            20                  25                  30          


Lys Lys Gly Lys His His Leu Trp Gly Ser Arg Leu Phe Asp Gln Ala 
        35                  40                  45              


Gln Thr Ala Gln Asn Arg Arg Asn Tyr Arg Ser Ser Arg Arg Arg Tyr 
    50                  55                  60                  


Asn Lys Arg Arg Gln Arg Ile Gly Leu Leu Arg Leu Ile Met Ser Asp 
65                  70                  75                  80  


Met Val Leu Glu Val Asp Pro Ser Phe Phe Ile Arg Leu Glu Lys Thr 
                85                  90                  95      


Thr Phe Leu Asp Lys Glu Asp Lys Lys Ala Ile Leu Lys Asp Asn Tyr 
            100                 105                 110         


Lys Met Asn Tyr Asn Leu Phe Cys Asp Glu Asp Tyr Asn Asp Lys Lys 
        115                 120                 125             


Tyr Phe Lys Asp Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Cys 
    130                 135                 140                 


Glu Ser Asp Glu Lys Ala Asp Pro Arg Leu Ile Tyr Leu Ala Leu His 
145                 150                 155                 160 


His Ile Val Lys Tyr Arg Gly Asn Phe Leu Tyr Glu Gly Gln Glu Leu 
                165                 170                 175     


His Leu Glu Pro Ser Asn Lys Glu Glu Asp Leu Lys Ile Leu Phe Asp 
            180                 185                 190         


Ile Leu Gly Lys Asn Asn Asp Thr Val Tyr Asp Ile Ser Glu Glu Gln 
        195                 200                 205             


Ile Gln Phe Ile Leu Lys Thr Val Val Glu Asn Ile Ser Lys Thr Ala 
    210                 215                 220                 


Lys Val Asp Glu Cys Met Ser Gln Leu Lys Leu Asn Ser Glu Asp Lys 
225                 230                 235                 240 


Lys Ile Val Lys Glu Phe Met Arg Gly Leu Val Gly Asn Lys Phe Asn 
                245                 250                 255     


Val Ser Lys Leu Tyr Met His Glu Asp Leu Gln Phe Asp Asp Glu Asp 
            260                 265                 270         


Leu Lys Leu Gln Phe Ser Asp Lys Ser Tyr Glu Glu Lys Ile Thr Glu 
        275                 280                 285             


Tyr Glu Asn Val Leu Glu Glu Lys Met Glu Phe Ile Asp Leu Met Gln 
    290                 295                 300                 


Arg Phe Tyr Ser Trp Ile Glu Leu Ser Lys Ile Val Gly Ser Asp Ser 
305                 310                 315                 320 


Gln His Ala Ser Ile Ser Gly Ala Met Val Asn Ile Tyr Glu Ser His 
                325                 330                 335     


Arg Glu Asp Leu Arg Thr Leu Lys Glu Val Met Leu Lys Ile Gly Lys 
            340                 345                 350         


Lys Glu Tyr Asp Glu Met Phe Lys Pro Thr Ser Lys Asn Val Val Asn 
        355                 360                 365             


Tyr Tyr Asn Tyr Val Asn Pro Val Ala Cys Ser Gly Asp Lys Thr Asp 
    370                 375                 380                 


Gly Phe Tyr Lys Tyr Val Lys Lys Ala Ile Glu Lys Ser Asp Asp Ser 
385                 390                 395                 400 


Arg Lys Asp Glu Ile Leu Gln Lys Ile Ala Asn Glu Thr Tyr Met Leu 
                405                 410                 415     


Lys Gln Thr Ser Lys Asn Asn Ala Tyr Ile Pro Tyr Gln Met Gln Lys 
            420                 425                 430         


Asp Glu Leu Ile Lys Ile Leu Asp His Gln Glu Lys Tyr Tyr Pro Val 
        435                 440                 445             


Leu Lys Glu Asn Arg Asp Lys Ile Ile Ser Ile Leu Glu Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Tyr Gly Pro Leu Asp Gly Asn Lys Gln Phe Gly Trp Leu 
465                 470                 475                 480 


Ile Lys Lys Lys Gly Lys Glu Asn Glu Arg Ile Leu Pro Trp Asn His 
                485                 490                 495     


Gln Glu Ile Val Asp Val Gln Glu Thr Ala Ala Gln Phe Ile Lys Lys 
            500                 505                 510         


Leu Thr Asn Tyr Cys Thr Tyr Leu Pro Ile Glu Lys Val Met Pro Gln 
        515                 520                 525             


Lys Ser Leu Thr Cys Ser Met Tyr Glu Val Leu Ser Glu Val Asn Lys 
    530                 535                 540                 


Ile Arg Ile Asp Gly Lys Leu Leu Pro Ile Asp Thr Lys Asn Arg Leu 
545                 550                 555                 560 


Ile Glu Asp Leu Phe Phe Lys Arg Lys Thr Val Lys Glu Lys Asp Leu 
                565                 570                 575     


Ile Asn Trp Leu Lys Gln Asn Gln Leu Thr Val Gly Glu Ile Thr Gly 
            580                 585                 590         


Tyr Gln Lys Glu Lys Ala Phe Ser Ser Ser Leu Ala Pro Trp Ile Asp 
        595                 600                 605             


Phe Lys Glu Ile Phe Asp Glu Ile Asn Asp Ser Asn Tyr Asp Leu Ile 
    610                 615                 620                 


Glu Lys Ile Ile Glu Asp Met Thr Ile Phe Asn Glu Gly Ser Ile Leu 
625                 630                 635                 640 


Lys Glu Arg Leu Lys Lys Ala Tyr Asn Leu Asp Gln Asn Lys Val Lys 
                645                 650                 655     


Lys Ile Met Lys Leu Lys Tyr Ser Gly Trp Ser Arg Leu Ser Lys Lys 
            660                 665                 670         


Leu Ile Asn Gly Ile Arg Ala Asp Asn Lys Phe Gly Ser Ser Val Ser 
        675                 680                 685             


Ile Leu Asp Val Met Lys Glu Ser His Met Ala Leu Met Glu Ile Ile 
    690                 695                 700                 


Asn Asp Gln Asp Leu Gly Phe Lys Gln Ile Ile Glu Lys Glu Asn Phe 
705                 710                 715                 720 


Lys Asn Glu Thr Gly Ser Phe Glu Tyr Glu Asp Ile Glu Asn Leu Ala 
                725                 730                 735     


Gly Ser Pro Ala Leu Lys Arg Gly Ile Trp Gln Thr Leu Gln Val Ile 
            740                 745                 750         


Glu Glu Ile Lys Asn Tyr Met Gly His Glu Pro Lys Asn Ile Tyr Ile 
        755                 760                 765             


Glu Phe Ala Arg Glu Glu Gln Glu Lys Val Arg Thr Thr Thr Arg Val 
    770                 775                 780                 


Lys Lys Leu Lys Ser Ile Tyr Asn Asp Ile Lys Asn Gln Leu Asp Val 
785                 790                 795                 800 


His Gly Lys Glu Val Tyr Ser Asn Leu Asn Lys Gln Asp Glu Lys Ser 
                805                 810                 815     


Ser Ile Glu Lys Arg Leu Tyr Leu Tyr Tyr Thr Gln Leu Gly Lys Cys 
            820                 825                 830         


Met Tyr Ser Gly Glu Ser Leu Asp Ile Asp Lys Leu Ser Asn Tyr Glu 
        835                 840                 845             


Ile Asp His Ile Phe Pro Arg Thr Leu Thr Ser Asp Asp Ser Leu Asp 
    850                 855                 860                 


Asn Leu Val Leu Val Lys Lys Lys Glu Asn Gln Arg Lys Leu Asp Asp 
865                 870                 875                 880 


Leu Val Leu Pro Leu Glu Ile Arg Asn Lys Met Glu Val Phe Trp Lys 
                885                 890                 895     


Lys Leu Tyr Asp Asn Gly Leu Ile Thr Gln Thr Lys Tyr Tyr Arg Leu 
            900                 905                 910         


Met Arg Asp Glu Phe Arg Arg Asp Gln Ile Asp Lys Phe Ile Asn Arg 
        915                 920                 925             


Gln Leu Val Glu Thr Arg Gln Ile Ile Lys His Val Ala Asn Ile Ile 
    930                 935                 940                 


Glu Asn His Tyr Glu Asp Thr Lys Val Phe Thr Val Arg Ala Asn Leu 
945                 950                 955                 960 


Ser His Glu Phe Arg Glu Lys Tyr Gly Ile Tyr Lys Asn Arg Asn Val 
                965                 970                 975     


Asn Asp Phe His His Ala His Asp Ala Tyr Ile Ala Cys Ile Ile Gly 
            980                 985                 990         


Arg Tyr Ile Gln Ile Arg Phe Pro  Gly Leu Glu Ala Lys  Tyr Val Tyr 
        995                 1000                 1005             


Gly Gln  Tyr Met Gln Asn Val  Lys Lys Thr Lys Asn  Asn Ile Asp 
    1010                 1015                 1020             


Lys Glu  Asn His Gly Phe Ile  Ile Asn Ser Met Lys  Tyr Glu Cys 
    1025                 1030                 1035             


Ile Asp  Glu Asp Thr Gly Glu  Val Ile Trp Asn Pro  Glu Arg Ile 
    1040                 1045                 1050             


Leu Asp  Tyr Ile Lys Cys Phe  Asn Tyr Arg Asp Val  Tyr Ile Thr 
    1055                 1060                 1065             


Lys Lys  Leu Asp Thr Asn Asn  Arg Glu Leu Phe Asn  Val Thr Ile 
    1070                 1075                 1080             


Leu Pro  Asn Asp Lys Asn Ser  Glu Lys Gly Lys Thr  Lys Ala Thr 
    1085                 1090                 1095             


Ile Pro  Val Asn Lys Leu Arg  Ser Asn Val Arg Lys  Tyr Gly Gly 
    1100                 1105                 1110             


Phe Ser  Gly Leu Gln Ser Asp  Ile Val Ala Ile Glu  Gly Lys Lys 
    1115                 1120                 1125             


Gly Lys  Lys Ile Asp Arg Arg  Leu Ile Asn Leu Pro  Ile Leu Leu 
    1130                 1135                 1140             


Arg Tyr  Val Ser Ile Glu Asp  Gln Cys Lys Tyr Ile  Met Glu Asn 
    1145                 1150                 1155             


Asn Lys  Tyr Ser Asp Val Lys  Ile Ile Lys Lys Ile  Lys Lys Asn 
    1160                 1165                 1170             


Gln Leu  Ile Glu Ile Asp Gly  Ile Cys Tyr Tyr Leu  Lys Ser Ala 
    1175                 1180                 1185             


Asp Glu  Leu Asp Thr Ala Tyr  Gln Leu Ile Leu Ser  Arg Asp Asp 
    1190                 1195                 1200             


Asn Glu  Met Ile Tyr Arg Ile  Asp Arg Ala Ile Gln  Asn Asn Asp 
    1205                 1210                 1215             


Phe Ser  Tyr Phe Asp Asn Lys  Lys Glu Asn Ile Asp  Glu Leu Tyr 
    1220                 1225                 1230             


Arg Leu  Ile Cys His Lys Met  Ile Glu Asn Tyr Pro  Lys Tyr Lys 
    1235                 1240                 1245             


Lys Lys  Val Tyr Glu Phe Ile  Lys Glu Arg Glu Glu  Glu Phe Phe 
    1250                 1255                 1260             


Glu Leu  Asp Asn Ser Gln Lys  Cys Ile Val Ile Phe  Glu Met Ile 
    1265                 1270                 1275             


Arg Val  Met Lys Arg Gly Glu  Gln Tyr Ile Asn Ile  Pro Phe Glu 
    1280                 1285                 1290             


Pro Tyr  Asn Leu Lys Asn Asp  Arg Lys Gly Arg Leu  Asn Cys Gln 
    1295                 1300                 1305             


Ser Ile  Leu Leu Asp Asn Thr  Tyr Phe Tyr Asp Val  Ser Ile Thr 
    1310                 1315                 1320             


Gly Ile  Tyr Ser Lys Lys Tyr  Lys Leu 
    1325                 1330         


<210>  833
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  833

Met Gln Lys Asn Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Met Lys Asp Asp Tyr Thr Leu Ile Arg Lys Arg Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Ile Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Lys Glu Thr Arg Leu 
    50                  55                  60                  


Lys Arg Gly Thr Arg Arg Arg Tyr Gln Arg Arg Arg Asn Arg Leu Ile 
65                  70                  75                  80  


Tyr Leu Gln Asp Ile Phe Gln Gln Pro Met Leu Ala Ile Asp Glu Asn 
                85                  90                  95      


Phe Phe His Arg Leu Asp Asp Ser Phe Phe Val Pro Asp Asp Lys Ser 
            100                 105                 110         


Tyr Asp Arg His Pro Ile Phe Gly Ser Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Asn Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Asn Pro Glu Lys Ala Asp Leu Arg Leu Val Tyr Thr Ala Leu Ala His 
145                 150                 155                 160 


Ile Val Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     


Glu Asn Thr Ser Ile Ser Glu Thr Phe Glu Gln Phe Leu Asp Thr Tyr 
            180                 185                 190         


Ser Asp Ile Phe Lys Glu Gln Leu Val Gly Asp Ile Ser Lys Val Glu 
        195                 200                 205             


Glu Ile Leu Ser Ser Lys Gln Ser Arg Ser Arg Lys His Glu Gln Ile 
    210                 215                 220                 


Met Ala Leu Phe Pro Asn Glu Asn Lys Leu Gly Asn Phe Gly Arg Phe 
225                 230                 235                 240 


Met Met Leu Ile Val Gly Asn Thr Ser Asn Phe Lys Pro Val Phe Asp 
                245                 250                 255     


Leu Asp Asp Glu Tyr Lys Leu Lys Leu Ser Asp Glu Thr Tyr Glu Glu 
            260                 265                 270         


Asp Leu Asp Thr Leu Leu Gly Met Thr Asp Asp Val Phe Leu Asp Val 
        275                 280                 285             


Phe Met Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Met Ser Ala Ile 
    290                 295                 300                 


Ile Ser Thr Asp Thr Gly Asn Ser Lys Ala Val Leu Ser Asn Gln Met 
305                 310                 315                 320 


Ile Asn Phe Tyr Asp Glu His Lys Val Asp Leu Ala Gln Leu Lys Gln 
                325                 330                 335     


Phe Phe Lys Thr His Leu Pro Asp Lys Tyr Tyr Glu Cys Phe Ser Asp 
            340                 345                 350         


Pro Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn Gln 
        355                 360                 365             


Glu Asp Phe Tyr Lys Tyr Ile Glu Lys Val Met Lys Thr Ile Lys Ser 
    370                 375                 380                 


Asp Lys Lys Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Val Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Ser Phe Tyr Asn Ser Val Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Gln Ala Ile Leu Asp Arg Gln Ser Gln Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Ala Glu Asn Arg Asp Lys Ile Glu Ser Leu Val Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Thr Val Ser Asp Gln Ser Glu Phe 
    450                 455                 460                 


Ala Trp Met Glu Arg Gln Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe 
465                 470                 475                 480 


Asp Glu Ile Val Asn Lys Glu Arg Ser Ala Glu Lys Phe Ile Glu Arg 
                485                 490                 495     


Met Thr Asn Met Asp Thr Tyr Leu Leu Glu Glu Lys Val Leu Pro Lys 
            500                 505                 510         


Arg Ser Leu Leu Tyr Gln Thr Phe Glu Val Tyr Asn Glu Leu Thr Lys 
        515                 520                 525             


Val Arg Tyr Thr Asn Glu Gln Gly Lys Thr Glu Lys Leu Asn Arg Gln 
    530                 535                 540                 


Gln Lys Ala Glu Ile Ile Glu Thr Leu Phe Lys Gln Lys Asn Arg Val 
545                 550                 555                 560 


Arg Glu Lys Asp Ile Ala Asn Tyr Leu Glu Gln Tyr Gly Tyr Val Asp 
                565                 570                 575     


Gly Thr Asp Ile Lys Gly Val Glu Asp Lys Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr Asn Asp Leu Ala Lys Ile Asp Gly Ala Lys Ala Tyr Leu Asp 
        595                 600                 605             


Asp Pro Glu Tyr Ala Asp Val Trp Glu Asp Ile Ile Lys Ile Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Lys Ala Met Arg Lys Lys Gln Leu Gln Thr Tyr Ser 
625                 630                 635                 640 


Asp Thr Leu Ser Pro Glu Ile Leu Lys Lys Leu Glu Arg Lys His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Phe Ser Lys Lys Leu Ile Asn Gly Leu Arg Asp 
            660                 665                 670         


Glu Gly Ser Asn Lys Thr Ile Leu Asp Tyr Leu Lys Ser Asp Glu Gly 
        675                 680                 685             


Ser Ser Gly Pro Thr Asn Arg Asn Phe Met Gln Leu Ile Arg Asp Asn 
    690                 695                 700                 


Thr Leu Ser Phe Lys Lys Lys Ile Glu Asp Ala Gln Thr Ile Glu Asp 
705                 710                 715                 720 


Thr Thr His Ile Tyr Asp Thr Val Ala Glu Leu Pro Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Arg Gln Ala Leu Lys Ile Val Glu Glu Ile Ile 
            740                 745                 750         


Asp Ile Ile Gly Tyr Glu Pro Glu Asn Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Ser Gln Thr Thr Lys Lys Gly Lys Asp Leu Ser Lys Glu Arg Leu 
    770                 775                 780                 


Glu Lys Leu Thr Glu Ala Ile Lys Glu Phe Asp Gly Pro Ser Asp Val 
785                 790                 795                 800 


Lys Val Lys Asp Leu Lys Asn Glu Asn Leu Arg Asn Asp Arg Leu Tyr 
                805                 810                 815     


Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Asn Glu Pro Leu 
            820                 825                 830         


Asp Ile Asn Asn Leu Ser Lys Tyr Asp Ile Asp His Ile Ile Pro Gln 
        835                 840                 845             


Ser Phe Thr Thr Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Arg 
    850                 855                 860                 


Thr Lys Asn Gln Gly Asn Lys Ser Asp Asp Val Pro Ser Ile Asn Ile 
865                 870                 875                 880 


Val His Lys Met Lys Pro Phe Trp Arg Gln Leu His Lys Ala Gly Leu 
                885                 890                 895     


Ile Ser Asp Arg Lys Phe Lys Asn Leu Thr Lys Ala Glu His Gly Gly 
            900                 905                 910         


Leu Thr Glu Ala Asp Arg Ala His Phe Leu Asn Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Asn Leu Leu Asp Ser Gln Tyr 
    930                 935                 940                 


Asn Thr Ala Glu Glu Gln Arg Ile Asn Ile Val Leu Leu Lys Ser Ser 
945                 950                 955                 960 


Met Thr Ser Arg Phe Arg Lys Glu Phe Lys Leu Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asp Tyr His His Gly His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Ala Thr Thr Ile Met Lys Val Tyr  Pro Asn Leu Lys Pro  Gln Phe Val 
        995                 1000                 1005             


Tyr Gly  Gln Tyr Lys Lys Thr  Ser Met Phe Lys Glu  Glu Lys Ala 
    1010                 1015                 1020             


Thr Ala  Arg Lys His Phe Tyr  Ser Asn Ile Thr Lys  Phe Phe Lys 
    1025                 1030                 1035             


Lys Glu  Lys Val Val Asn Glu  Glu Thr Gly Glu Ile  Leu Trp Asp 
    1040                 1045                 1050             


Thr Glu  Arg His Leu Ser Thr  Ile Lys Arg Val Leu  Ser Trp Lys 
    1055                 1060                 1065             


Gln Met  Asn Ile Val Lys Lys  Val Glu Lys Gln Lys  Gly Gln Leu 
    1070                 1075                 1080             


Trp Lys  Glu Thr Ile Tyr Pro  Lys Gly Asp Ser Ser  Lys Leu Ile 
    1085                 1090                 1095             


Pro Val  Lys Glu Gly Met Asp  Pro Gln Lys Tyr Gly  Gly Leu Ser 
    1100                 1105                 1110             


Gln Val  Ser Glu Ala Phe Ala  Val Val Ile Thr His  Glu Lys Gly 
    1115                 1120                 1125             


Lys Lys  Lys Gln Leu Lys Ser  Asp Leu Ile Ser Ile  Pro Ile Val 
    1130                 1135                 1140             


Asp Gln  Lys Ala Tyr Glu Gln  His Pro Thr Ala Tyr  Leu Glu Glu 
    1145                 1150                 1155             


Ala Gly  Tyr Asn Asn Pro Thr  Val Leu His Glu Leu  Phe Lys Tyr 
    1160                 1165                 1170             


Gln Leu  Phe Glu Leu Glu Asp  Gly Ser Arg Arg Met  Ile Ala Ser 
    1175                 1180                 1185             


Ala Lys  Glu Phe Gln Lys Gly  Asn Gln Met Val Leu  Pro Leu Glu 
    1190                 1195                 1200             


Leu Val  Glu Leu Leu Tyr His  Ala Asn Arg Tyr Asp  Lys Val Lys 
    1205                 1210                 1215             


Phe Pro  Asp Ser Ile Glu Tyr  Val His Asp Asn Leu  Ala Lys Phe 
    1220                 1225                 1230             


Asp Asp  Leu Leu Glu Tyr Val  Ile Asp Phe Ser Asn  Lys Tyr Ile 
    1235                 1240                 1245             


Asn Ala  Asp Lys Asn Val Gln  Lys Ile Gln Lys Ile  Tyr Lys Glu 
    1250                 1255                 1260             


His Gly  Thr Glu Asp Val Glu  Leu Thr Val Glu Ser  Phe Val Asn 
    1265                 1270                 1275             


Leu Met  Thr Phe Thr Ala Met  Gly Ala Pro Ala Thr  Phe Lys Phe 
    1280                 1285                 1290             


Tyr Gly  Glu Ser Ile Thr Arg  Ser Arg Tyr Thr Ser  Ile Thr Glu 
    1295                 1300                 1305             


Phe Arg  Gly Ser Thr Leu Ile  Phe Gln Ser Ile Thr  Gly Leu Tyr 
    1310                 1315                 1320             


Glu Thr  Arg Tyr Lys Leu Glu  Asp Asn 
    1325                 1330         


<210>  834
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  834

Met Met Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Ser Ser Ser Val 
1               5                   10                  15      


Gly Phe Val Ala Val Asp Asp Gln Tyr Arg Val Val Arg Ala Lys Gly 
            20                  25                  30          


Lys Asn Val Ile Gly Thr Arg Leu Phe Asp Glu Gly Lys Thr Ala Ala 
        35                  40                  45              


Asp Arg Arg Gly Leu Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Gly Ile Phe Glu Pro His Ile Asn Glu 
65                  70                  75                  80  


Ile Asp Glu Ser Phe Phe Met Arg Leu Lys Glu Ser Asn Ile Ser Pro 
                85                  90                  95      


Lys Asp Ser Asn Lys His Phe Pro Gly Asp Ile Leu Phe Asn Asp Arg 
            100                 105                 110         


Ser Asp Arg Glu Phe Tyr Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Gln Ala Leu Lys Thr Glu Lys Arg Gln Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Met His His Ile Val Lys Tyr Arg Gly His Phe Leu Glu Gly 
145                 150                 155                 160 


Gly Thr Val Thr Asp Phe Glu Val Gly Glu Leu Asp Leu Ala Gly Lys 
                165                 170                 175     


Phe Ser Arg Leu Asn Glu Leu Phe Glu Ser Ile Asn Leu Glu Val Pro 
            180                 185                 190         


Phe Val Leu Arg Leu Asp Asn Leu Glu Asp Val Lys Ala Val Leu Ile 
        195                 200                 205             


Asp Ser Gln Arg Ser Ala Gln Asp Arg Gln Arg Thr Leu Thr Pro Asp 
    210                 215                 220                 


Ile Tyr Val Pro Ser Thr Asp Lys Ile Thr Glu Lys Arg Phe Lys Gly 
225                 230                 235                 240 


Ile Ala Thr Glu Leu Leu Lys Ala Ile Val Gly Asn Lys Ala Lys Phe 
                245                 250                 255     


Asp Val Leu Ala Gly Leu Glu Leu Asp Lys Glu Glu Ala Lys Leu Trp 
            260                 265                 270         


Ala Leu Thr Phe Asp Ser Glu Thr Phe Asp Glu Asp Ile Glu Gln Leu 
        275                 280                 285             


Glu Gly Asp Met Thr Glu Ala Thr Gln Asp Leu Ile Glu Ile Leu Lys 
    290                 295                 300                 


Ser Leu Arg Ser Leu Ile Arg Leu Ala Val Asn Met Pro Glu Gly Val 
305                 310                 315                 320 


Ser Leu Ser Glu Met Met Ile Gln Arg Tyr Glu Asp His Ala Asp His 
                325                 330                 335     


Leu Lys Leu Leu Arg Asp Ile Ile His Asn Thr Asp Ala Glu Lys Ala 
            340                 345                 350         


Ala Gly Leu Gln Ala Ala Tyr Asp Gly Tyr Ile Asp Gly Val Lys Gly 
        355                 360                 365             


Lys Lys Thr Pro Val Asp Asp Phe Tyr Lys Ala Ile Lys Lys Asn Leu 
    370                 375                 380                 


Asp Asp Ser Glu Ala Ala His Gln Ile Leu Ala Leu Ile Asp Lys Asn 
385                 390                 395                 400 


Glu Phe Met Pro Lys Gln Arg Ser Lys Ser Asn Gly Trp Ile Pro His 
                405                 410                 415     


Gln Ile Gln Gln Lys Glu Leu Asp Arg Ile Ile Glu Asn Gln Lys Gln 
            420                 425                 430         


Tyr Tyr Pro Trp Leu Ala Asp Val Asn Pro Asn Glu Ser Arg Arg Lys 
        435                 440                 445             


Val Ala Pro Tyr Lys Ile Asp Glu Leu Val Thr Phe Arg Val Pro Tyr 
    450                 455                 460                 


Tyr Val Gly Pro Leu Val Thr Ser Glu Glu Gln Lys Lys Tyr Gly Ser 
465                 470                 475                 480 


Lys Phe Ala Trp Met Val Arg Lys Glu Glu Lys Gly Glu Ile Thr Pro 
                485                 490                 495     


Trp Asn Phe Asp Gln Lys Val Asp Arg Ala Ala Ser Ala Thr Glu Phe 
            500                 505                 510         


Ile Asn Arg Met Thr Thr Met Asp Thr Tyr Leu Leu Asn Glu Asp Val 
        515                 520                 525             


Leu Pro Ala His Ser Leu Ile Tyr Gln Lys Tyr Glu Val Leu Asn Glu 
    530                 535                 540                 


Leu Asn Lys Val Lys Ile Asn Gly Asp Ala Ile Thr Pro Glu Leu Lys 
545                 550                 555                 560 


Gln Lys Ile Tyr His Asp Leu Phe Glu Lys Gln Thr Thr Val Ser Ile 
                565                 570                 575     


Lys Asn Leu Gln Thr Tyr Leu Val Ser Glu Ser Glu Ala Ser Glu Val 
            580                 585                 590         


Pro Glu Ile Lys Gly Leu Ala Asp Glu Lys Lys Phe Leu Ser Gly Leu 
        595                 600                 605             


Thr Thr Tyr His Asp Leu Met Lys Ile Phe Asp Arg Asn Val Val Asn 
    610                 615                 620                 


Asp Pro Glu Arg Ala Glu Asp Phe Glu Lys Ile Ile Arg Trp Ser Thr 
625                 630                 635                 640 


Val Phe Glu Asp Lys His Ile Tyr Arg Glu Lys Leu Asp Ser Leu Ser 
                645                 650                 655     


Trp Leu Thr Thr Asp Gln Lys Asp Arg Leu Ala Val Lys Arg Tyr Arg 
            660                 665                 670         


Gly Trp Gly Arg Leu Ser Glu Lys Leu Leu Ile Gly Leu Arg Asp Glu 
        675                 680                 685             


Gly His Arg Ser Ile Met Asp Tyr Leu Trp Glu Thr Thr Ser Asn Phe 
    690                 695                 700                 


Met Gln Ile Gln Ala Gln Asn Asp Phe Ser Glu Lys Ile Ala Ala Glu 
705                 710                 715                 720 


Asn Lys Glu Phe Leu Asp Asn Thr Gln Ser Val Gln Gly Ile Ile Asn 
                725                 730                 735     


Glu Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Thr Leu 
            740                 745                 750         


Leu Val Val Glu Asp Ile Lys Lys Ala Phe His Gly Glu Ala Pro Lys 
        755                 760                 765             


Arg Ile Phe Val Glu Phe Ala Arg Glu Asn Glu Ser Thr Pro Gln Arg 
    770                 775                 780                 


Ser Ile Gln Arg Gln Arg Gln Val Glu Asn Ala Tyr Glu Lys Val Thr 
785                 790                 795                 800 


Gln Asn Leu Leu Val Asp Asp Lys Ile Arg Ala Glu Leu Lys Asp Ser 
                805                 810                 815     


Ile Asn Glu Lys Arg Leu Phe His Asp Arg Leu Phe Leu Tyr Phe Met 
            820                 825                 830         


Gln Gly Gly Ile Asp Met Tyr Ser Gly Lys Lys Ile Asn Ile Asp Asn 
        835                 840                 845             


Leu Ser Ala Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe Thr Val 
    850                 855                 860                 


Asp Asn Ser Leu Asp Asn Arg Val Leu Thr Asn Gln Lys Asp Asn Arg 
865                 870                 875                 880 


Glu Lys Gly Asn Ser Leu Pro Ser Glu Leu Tyr Arg Gly Ser Met Lys 
                885                 890                 895     


Glu Thr Trp Glu Met Met Arg Asn Gln Gly Leu Leu Ser Lys Gly Lys 
            900                 905                 910         


Phe His Arg Leu Met Met Thr Pro Asn Ser Met Thr Ala Tyr Asp Gln 
        915                 920                 925             


Gln Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val Ile Lys 
    930                 935                 940                 


Leu Ala Val Glu Ile Leu Ala Ser Glu Phe Asp Gln Ala Asp Glu Lys 
945                 950                 955                 960 


Val Glu Ile Val Ser Val Lys Ala Gly Leu Thr His Gln Met Arg Gln 
                965                 970                 975     


Asp Phe Ser Leu Ile Lys Asn Arg Asp Val Asn Asp Tyr His His Ala 
            980                 985                 990         


Phe Asp Ala Tyr Leu Thr Ala Phe  Val Gly Thr Tyr Leu  Leu Lys Arg 
        995                 1000                 1005             


Tyr Pro  Lys Leu Arg Pro Tyr  Phe Val Tyr Gly Glu  Phe Lys Lys 
    1010                 1015                 1020             


Phe Ser  Gln Lys Gln Ala Asn  Leu Thr Asn Phe Asn  Phe Leu Trp 
    1025                 1030                 1035             


Ala Leu  Lys His Lys Asp Gln  Val Ala Val Glu Asp  Thr Gly Glu 
    1040                 1045                 1050             


Lys Ile  Trp Asn Lys Pro Gln  Asp Leu Ala Tyr Ile  Arg His Leu 
    1055                 1060                 1065             


Phe Ala  Phe Lys Lys Ile Leu  Ile Ser Arg Glu Val  Ser Glu Arg 
    1070                 1075                 1080             


His Gly  Ala Leu Phe Asp Gln  Thr Leu Tyr Lys Ala  Ser Asp Asp 
    1085                 1090                 1095             


Lys Ala  Ser Gly Gln Gly Ser  Lys Lys Leu Ile Pro  Ala Lys Asp 
    1100                 1105                 1110             


Asn Arg  Pro Thr Glu Leu Tyr  Gly Gly Tyr Ser Gly  Gln Thr Ser 
    1115                 1120                 1125             


Ala Phe  Met Ala Ile Val Lys  Val Thr Lys Gly Lys  Lys Thr Lys 
    1130                 1135                 1140             


Tyr Leu  Val Val Gly Val Pro  Thr Ala Ala Ile Ser  Gln Leu Gln 
    1145                 1150                 1155             


Gly Met  Asp Val Lys Ser Ser  Gln Tyr Thr Glu Lys  Ile Tyr Asn 
    1160                 1165                 1170             


Ile Leu  Ser Pro Lys Leu Leu  Ser Lys Asn Asp Asp  Phe Glu Val 
    1175                 1180                 1185             


Ile Val  Pro Lys Val Arg Tyr  Arg Gln Leu Ile Leu  Asp Asn Asn 
    1190                 1195                 1200             


Gln Leu  Phe Thr Leu Gly Ser  Asp Thr Tyr Lys Tyr  Asn Ala Arg 
    1205                 1210                 1215             


Gln Leu  Val Leu Ser Asn Glu  Val Leu Ala Val Ile  Asn Arg Asp 
    1220                 1225                 1230             


Lys Ser  Phe Ile Asp Lys Lys  Leu Ser Glu Glu Ala  Glu Asn Gln 
    1235                 1240                 1245             


Ala Leu  Asn Asp Ala Phe Asp  Glu Ile Val Val Ala  Met Asp Lys 
    1250                 1255                 1260             


His Phe  Asp Leu Tyr Asp Lys  Asn Gly Phe Arg Glu  Lys Val Lys 
    1265                 1270                 1275             


Ala Gly  Lys Gln Arg Phe Glu  Glu Leu Pro Val Ser  Leu Phe Ser 
    1280                 1285                 1290             


Glu Asp  Lys Glu Asn Glu Gly  Ile Thr Lys Arg Thr  Val Leu Lys 
    1295                 1300                 1305             


Arg Ile  Leu Gln Gly Leu His  Ala Asn Ala Thr Thr  Ser Asp Leu 
    1310                 1315                 1320             


Lys Val  Leu Asp Gly Lys Phe  Tyr Asn 
    1325                 1330         


<210>  835
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  835

Met Lys Asn Tyr Thr Ile Gly Leu Asp Ile Gly Val Ala Ser Val Gly 
1               5                   10                  15      


Trp Val Cys Ile Asp Glu Asn Tyr Lys Ile Leu Asn Tyr Asn Asn Arg 
            20                  25                  30          


His Ala Phe Gly Val His Glu Phe Glu Ser Ala Glu Ser Ala Ala Gly 
        35                  40                  45              


Arg Arg Leu Lys Arg Gly Met Arg Arg Arg Tyr Asn Arg Arg Lys Lys 
    50                  55                  60                  


Arg Leu Gln Leu Leu Gln Ser Leu Phe Asp Ser Tyr Ile Thr Asp Ser 
65                  70                  75                  80  


Gly Phe Phe Ser Lys Thr Asp Ser Gln His Phe Trp Lys Asn Asn Asn 
                85                  90                  95      


Glu Phe Glu Asn Arg Ser Leu Thr Glu Val Leu Ser Ser Leu Arg Ile 
            100                 105                 110         


Ser Ser Arg Lys Tyr Pro Thr Ile Tyr His Leu Arg Ser Asp Leu Ile 
        115                 120                 125             


Glu Ser Asn Lys Lys Met Asp Leu Arg Leu Val Tyr Leu Ala Leu His 
    130                 135                 140                 


Asn Leu Val Lys Tyr Arg Gly His Phe Leu Gln Glu Gly Asn Trp Ser 
145                 150                 155                 160 


Glu Ala Ala Ser Ala Glu Gly Met Asp Asp Gln Leu Leu Glu Leu Val 
                165                 170                 175     


Thr Arg Tyr Ala Glu Leu Glu Asn Leu Ser Pro Leu Asp Leu Ser Glu 
            180                 185                 190         


Ser Gln Trp Lys Ala Ala Glu Thr Leu Leu Leu Asn Arg Asn Leu Thr 
        195                 200                 205             


Lys Thr Asp Gln Ser Lys Glu Leu Thr Ala Met Phe Gly Lys Glu Tyr 
    210                 215                 220                 


Glu Pro Phe Cys Lys Leu Val Ala Gly Leu Gly Val Ser Leu His Gln 
225                 230                 235                 240 


Leu Phe Pro Ser Ser Glu Gln Ala Leu Ala Tyr Lys Glu Thr Lys Thr 
                245                 250                 255     


Lys Val Gln Leu Ser Asn Glu Asn Val Glu Glu Val Met Glu Leu Leu 
            260                 265                 270         


Leu Glu Glu Glu Ser Ala Leu Leu Glu Ala Val Gln Pro Phe Tyr Gln 
        275                 280                 285             


Gln Val Val Leu Tyr Glu Leu Leu Lys Gly Glu Thr Tyr Val Ala Lys 
    290                 295                 300                 


Ala Lys Val Ser Ala Phe Lys Gln Tyr Gln Lys Asp Met Ala Ser Leu 
305                 310                 315                 320 


Lys Asn Leu Leu Asp Lys Thr Phe Gly Glu Lys Val Tyr Arg Ser Tyr 
                325                 330                 335     


Phe Ile Ser Asp Lys Asn Ser Gln Arg Glu Tyr Gln Lys Ser His Lys 
            340                 345                 350         


Val Glu Val Leu Cys Lys Leu Asp Gln Phe Asn Lys Glu Ala Lys Phe 
        355                 360                 365             


Ala Glu Thr Phe Tyr Lys Asp Leu Lys Lys Leu Leu Glu Asp Lys Ser 
    370                 375                 380                 


Lys Thr Ser Ile Gly Thr Thr Glu Lys Asp Glu Met Leu Arg Ile Ile 
385                 390                 395                 400 


Lys Ala Ile Asp Ser Asn Gln Phe Leu Gln Lys Gln Lys Gly Ile Gln 
                405                 410                 415     


Asn Ala Ala Ile Pro His Gln Asn Ser Leu Tyr Glu Ala Glu Lys Ile 
            420                 425                 430         


Leu Arg Asn Gln Gln Ala His Tyr Pro Phe Ile Thr Thr Glu Trp Ile 
        435                 440                 445             


Glu Lys Val Lys Gln Ile Leu Ala Phe Arg Ile Pro Tyr Tyr Ile Gly 
    450                 455                 460                 


Pro Leu Val Lys Asp Thr Thr Gln Ser Pro Phe Ser Trp Val Glu Arg 
465                 470                 475                 480 


Lys Gly Asp Ala Pro Ile Thr Pro Trp Asn Phe Asp Glu Gln Ile Asp 
                485                 490                 495     


Lys Ala Ala Ser Ala Glu Ala Phe Ile Ser Arg Met Arg Lys Thr Cys 
            500                 505                 510         


Thr Tyr Leu Lys Gly Gln Glu Val Leu Pro Lys Ser Ser Leu Thr Tyr 
        515                 520                 525             


Glu Arg Phe Glu Val Leu Asn Glu Leu Asn Gly Ile Gln Leu Arg Thr 
    530                 535                 540                 


Thr Gly Ala Glu Ser Asp Phe Arg His Arg Leu Ser Tyr Glu Met Lys 
545                 550                 555                 560 


Cys Trp Ile Ile Asp Asn Val Phe Lys Gln Tyr Lys Thr Val Ser Thr 
                565                 570                 575     


Lys Arg Leu Leu Gln Glu Leu Lys Lys Ser Pro Tyr Ala Asp Glu Leu 
            580                 585                 590         


Tyr Asp Glu His Thr Gly Glu Ile Lys Glu Val Phe Gly Thr Gln Lys 
        595                 600                 605             


Glu Asn Ala Phe Ala Thr Ser Leu Ser Gly Tyr Ile Ser Met Lys Ser 
    610                 615                 620                 


Ile Leu Gly Ala Val Val Asp Asp Asn Pro Ala Met Thr Glu Glu Leu 
625                 630                 635                 640 


Ile Tyr Trp Ile Ala Val Phe Glu Asp Arg Glu Ile Leu His Leu Lys 
                645                 650                 655     


Ile Gln Glu Lys Tyr Pro Ser Ile Thr Asp Val Gln Arg Gln Lys Leu 
            660                 665                 670         


Ala Leu Val Lys Leu Pro Gly Trp Gly Arg Phe Ser Arg Leu Leu Ile 
        675                 680                 685             


Asp Gly Leu Pro Leu Asp Glu Gln Gly Gln Ser Val Leu Asp His Met 
    690                 695                 700                 


Glu Gln Tyr Ser Ser Val Phe Met Glu Val Leu Lys Asn Lys Gly Phe 
705                 710                 715                 720 


Gly Leu Glu Lys Lys Ile Gln Lys Met Asn Gln His Gln Val Asp Gly 
                725                 730                 735     


Thr Lys Lys Ile Arg Tyr Glu Asp Ile Glu Glu Leu Ala Gly Ser Pro 
            740                 745                 750         


Ala Leu Lys Arg Gly Ile Trp Arg Ser Val Lys Ile Val Glu Glu Leu 
        755                 760                 765             


Val Ser Ile Phe Gly Glu Pro Ala Asn Ile Val Leu Glu Val Ala Arg 
    770                 775                 780                 


Glu Asp Gly Glu Lys Lys Arg Thr Lys Ser Arg Lys Asp Gln Trp Glu 
785                 790                 795                 800 


Glu Leu Thr Lys Thr Thr Leu Lys Asn Asp Pro Asp Leu Lys Ser Phe 
                805                 810                 815     


Ile Gly Glu Ile Lys Ser Gln Gly Asp Gln Arg Phe Asn Glu Gln Arg 
            820                 825                 830         


Phe Trp Leu Tyr Val Thr Gln Gln Gly Lys Cys Leu Tyr Thr Gly Lys 
        835                 840                 845             


Ala Leu Asp Ile Gln Asn Leu Ser Met Tyr Glu Val Asp His Ile Leu 
    850                 855                 860                 


Pro Gln Asn Phe Val Lys Asp Asp Ser Leu Asp Asn Leu Ala Leu Val 
865                 870                 875                 880 


Met Pro Glu Ala Asn Gln Arg Lys Asn Gln Val Gly Gln Asn Lys Met 
                885                 890                 895     


Pro Leu Glu Ile Ile Glu Ala Asn Gln Gln Tyr Ala Met Arg Thr Leu 
            900                 905                 910         


Trp Glu Arg Leu His Glu Leu Lys Leu Ile Ser Ser Gly Lys Leu Gly 
        915                 920                 925             


Arg Leu Lys Lys Pro Ser Phe Asp Glu Val Asp Lys Asp Lys Phe Ile 
    930                 935                 940                 


Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys His Val Arg Asp 
945                 950                 955                 960 


Leu Leu Asp Glu Arg Phe Ser Lys Ser Asp Ile His Leu Val Lys Ala 
                965                 970                 975     


Gly Ile Val Ser Lys Phe Arg Arg Phe Ser Glu Ile Pro Lys Ile Arg 
            980                 985                 990         


Asp Tyr Asn Asn Lys His His Ala  Met Asp Ala Leu Phe  Ala Ala Ala 
        995                 1000                 1005             


Leu Ile  Gln Ser Ile Leu Gly  Lys Tyr Gly Lys Asn  Phe Leu Ala 
    1010                 1015                 1020             


Phe Asp  Leu Ser Lys Lys Asp  Arg Gln Lys Gln Trp  Arg Ser Val 
    1025                 1030                 1035             


Lys Gly  Ser Asn Lys Glu Phe  Phe Leu Phe Lys Asn  Phe Gly Asn 
    1040                 1045                 1050             


Leu Arg  Leu Gln Ser Pro Val  Thr Gly Glu Glu Val  Ser Gly Val 
    1055                 1060                 1065             


Glu Tyr  Met Lys His Val Tyr  Phe Glu Leu Pro Trp  Gln Thr Thr 
    1070                 1075                 1080             


Lys Met  Thr Gln Thr Gly Asp  Gly Met Phe Tyr Lys  Glu Ser Ile 
    1085                 1090                 1095             


Phe Ser  Pro Lys Val Lys Gln  Ala Lys Tyr Val Ser  Pro Lys Thr 
    1100                 1105                 1110             


Glu Lys  Phe Val His Asp Glu  Val Lys Asn His Ser  Ile Cys Leu 
    1115                 1120                 1125             


Val Glu  Phe Thr Phe Met Lys  Lys Glu Lys Glu Val  Gln Glu Thr 
    1130                 1135                 1140             


Lys Phe  Ile Asp Leu Lys Val  Ile Glu His His Gln  Phe Leu Lys 
    1145                 1150                 1155             


Glu Pro  Glu Ser Gln Leu Ala  Lys Phe Leu Ala Glu  Lys Glu Thr 
    1160                 1165                 1170             


Asn Ser  Pro Ile Ile His Ala  Arg Ile Ile Arg Thr  Ile Pro Lys 
    1175                 1180                 1185             


Tyr Gln  Lys Ile Trp Ile Glu  His Phe Pro Tyr Tyr  Phe Ile Ser 
    1190                 1195                 1200             


Thr Arg  Glu Leu His Asn Ala  Arg Gln Phe Glu Ile  Ser Tyr Glu 
    1205                 1210                 1215             


Leu Met  Glu Lys Val Lys Gln  Leu Ser Glu Arg Ser  Ser Val Glu 
    1220                 1225                 1230             


Glu Leu  Lys Ile Val Phe Gly  Leu Leu Ile Asp Gln  Met Asn Asp 
    1235                 1240                 1245             


Asn Tyr  Pro Ile Tyr Thr Lys  Ser Ser Ile Gln Asp  Arg Val Gln 
    1250                 1255                 1260             


Lys Phe  Val Asp Thr Gln Leu  Tyr Asp Phe Lys Ser  Phe Glu Ile 
    1265                 1270                 1275             


Gly Phe  Glu Glu Leu Lys Lys  Ala Val Ala Ala Asn  Ala Gln Arg 
    1280                 1285                 1290             


Ser Asp  Thr Phe Gly Ser Arg  Ile Ser Lys Lys Pro  Lys Pro Glu 
    1295                 1300                 1305             


Glu Val  Ala Ile Gly Tyr Glu  Ser Ile Thr Gly Leu  Lys Tyr Arg 
    1310                 1315                 1320             


Lys Pro  Arg Ser Val Val Gly  Thr Lys Arg 
    1325                 1330             


<210>  836
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  836

Met Ser Gln Asn Ile Val Asp Tyr Cys Ile Gly Leu Asp Leu Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Val Asp Met Asn His Arg Leu Met Lys 
            20                  25                  30          


Arg Asn Gly Lys His Leu Trp Gly Ser Arg Leu Phe Ser Asn Ala Glu 
        35                  40                  45              


Thr Ala Ala Asn Arg Arg Ala Ser Arg Ser Ile Arg Arg Arg Tyr Asn 
    50                  55                  60                  


Lys Arg Arg Glu Arg Ile Arg Leu Leu Arg Ala Ile Leu Gln Asp Met 
65                  70                  75                  80  


Val Leu Glu Asn Asp Pro Thr Phe Phe Ile Arg Leu Glu His Thr Ser 
                85                  90                  95      


Phe Leu Asp Glu Glu Asp Lys Ala Asn Tyr Leu Gly Ala Asp Tyr Lys 
            100                 105                 110         


Asp Asn Tyr Asn Leu Phe Ile Asp Glu Asp Phe Asn Asp Tyr Thr Tyr 
        115                 120                 125             


Tyr His Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Ala Leu Cys Glu 
    130                 135                 140                 


Ser Thr Glu Lys Ala Asp Pro Arg Leu Ile Tyr Leu Ala Leu His His 
145                 150                 155                 160 


Ile Val Lys Tyr Arg Gly Asn Phe Leu Tyr Glu Gly Gln Lys Phe Asn 
                165                 170                 175     


Met Asp Ala Ser Asn Ile Glu Asp Arg Leu Ser Asp Val Phe Thr Gln 
            180                 185                 190         


Phe Ala Asp Phe Asn Asn Ile Pro Tyr Glu Asp Asp Glu Lys Lys Asn 
        195                 200                 205             


Leu Glu Ile Leu Glu Ile Leu Lys Lys Pro Leu Ser Lys Lys Ala Lys 
    210                 215                 220                 


Val Asp Glu Val Met Ala Leu Ile Ala Pro Glu Lys Asp Phe Lys Ser 
225                 230                 235                 240 


Ala Tyr Lys Glu Leu Val Thr Gly Ile Ala Gly Asn Lys Met Asn Val 
                245                 250                 255     


Thr Lys Met Ile Leu Cys Glu Pro Ile Lys Gln Gly Asp Ser Glu Ile 
            260                 265                 270         


Lys Leu Lys Phe Ser Asp Ser Asn Tyr Asp Asp Gln Phe Ser Glu Val 
        275                 280                 285             


Glu Asn Asp Leu Gly Glu Tyr Val Glu Phe Ile Asp Ser Leu His Asn 
    290                 295                 300                 


Ile Tyr Ser Trp Val Glu Leu Gln Thr Ile Met Gly Ala Thr His Thr 
305                 310                 315                 320 


Tyr Asn Ala Ser Ile Ser Glu Ala Met Val Ser Arg Tyr Asn Lys His 
                325                 330                 335     


His Glu Asp Leu Gln Leu Leu Lys Lys Cys Ile Lys Asp Asn Val Pro 
            340                 345                 350         


Lys Lys Tyr Phe Asp Met Phe Arg Asn Asp Ser Glu Lys Leu Lys Gly 
        355                 360                 365             


Tyr Tyr Asn Tyr Ile Asn His Pro Ser Lys Ala Pro Val Asp Glu Phe 
    370                 375                 380                 


Tyr Lys Tyr Val Lys Lys Cys Ile Glu Lys Val Asp Thr Pro Glu Ala 
385                 390                 395                 400 


Lys Gln Ile Leu His Asp Ile Glu Leu Glu Asn Phe Leu Leu Lys Gln 
                405                 410                 415     


Asn Ser Arg Thr Asn Gly Ser Val Pro Tyr Gln Met Gln Leu Asp Glu 
            420                 425                 430         


Met Ile Lys Ile Ile Asp Asn Gln Ala Lys Tyr Tyr Pro Val Leu Lys 
        435                 440                 445             


Glu Lys Arg Glu Gln Leu Leu Ser Ile Leu Thr Phe Lys Ile Pro Tyr 
    450                 455                 460                 


Tyr Phe Gly Pro Leu Asn Glu Thr Ser Glu His Ala Trp Ile Lys Arg 
465                 470                 475                 480 


Leu Glu Gly Lys Glu Asn Gln Arg Ile Leu Pro Trp Asn Tyr Gln Asp 
                485                 490                 495     


Ile Val Asp Val Asp Ala Thr Ala Glu Gly Phe Ile Lys Arg Met Arg 
            500                 505                 510         


Ser Tyr Cys Thr Tyr Phe Pro Asp Glu Glu Val Leu Pro Lys Asn Ser 
        515                 520                 525             


Leu Ile Val Ser Lys Tyr Glu Val Tyr Asn Glu Leu Asn Lys Ile Arg 
    530                 535                 540                 


Val Asp Asp Lys Leu Leu Glu Val Asp Ile Lys Asn Asp Ile Tyr Asn 
545                 550                 555                 560 


Glu Leu Phe Met Asn Asn Lys Thr Val Thr Glu Lys Lys Leu Lys Asn 
                565                 570                 575     


Trp Leu Val Asn Asn Gln Cys Cys Asn Lys Asn Ala Glu Ile Lys Gly 
            580                 585                 590         


Phe Gln Lys Glu Asn Gln Phe Ser Thr Ser Leu Thr Pro Trp Ile Asp 
        595                 600                 605             


Phe Thr Asn Ile Phe Gly Glu Ile Asn Gln Ser Asn Phe Asp Leu Ile 
    610                 615                 620                 


Glu Asp Ile Ile Tyr Asp Leu Thr Val Phe Glu Asp Lys Lys Ile Met 
625                 630                 635                 640 


Lys Arg Arg Leu Lys Lys Lys Tyr Ala Leu Pro Asp Asp Lys Ile Lys 
                645                 650                 655     


Gln Ile Leu Lys Leu Lys Tyr Lys Asp Trp Ser Arg Leu Ser Lys Lys 
            660                 665                 670         


Leu Leu Asp Gly Ile Val Ala Asp Asn Lys Phe Gly Ser Ser Val Thr 
        675                 680                 685             


Val Leu Asp Val Leu Glu Met Ser Arg Leu Asn Leu Met Glu Ile Ile 
    690                 695                 700                 


Asn Asp Arg Asp Leu Gly Tyr Ala Gln Met Ile Glu Ala Ala Ala Ser 
705                 710                 715                 720 


Cys Pro Glu Asp Gly Lys Phe Thr Tyr Lys Glu Val Gln Arg Leu Ala 
                725                 730                 735     


Gly Ser Pro Ala Leu Lys Arg Gly Ile Trp Gln Ser Leu Gln Ile Val 
            740                 745                 750         


Glu Glu Ile Thr Lys Val Met Lys Cys Arg Pro Lys Tyr Ile Tyr Ile 
        755                 760                 765             


Glu Phe Glu Arg Ser Glu Glu Thr Lys Glu Arg Thr Glu Ser Lys Ile 
    770                 775                 780                 


Lys Lys Leu Glu Asn Val Tyr Lys Asp Leu Asp Glu Gln Thr Lys Val 
785                 790                 795                 800 


Glu Tyr Lys Thr Val Leu Glu Glu Leu Lys Gly Phe Asp Asn Thr Lys 
                805                 810                 815     


Lys Ile Ser Ser Asp Ser Leu Phe Leu Tyr Phe Thr Gln Leu Gly Lys 
            820                 825                 830         


Cys Met Tyr Ser Gly Lys Lys Leu Asp Ile Asp Ser Leu Asp Lys Tyr 
        835                 840                 845             


Gln Ile Asp His Ile Val Pro Gln Ser Leu Val Lys Asp Asp Ser Phe 
    850                 855                 860                 


Asp Asn Arg Val Leu Val Val Pro Ser Glu Asn Gln Arg Lys Leu Asp 
865                 870                 875                 880 


Asp Leu Val Val Pro Ser Asp Ile Arg Val Lys Met Asn Ser Phe Trp 
                885                 890                 895     


Lys Leu Leu Phe Asp His Glu Leu Ile Ser Pro Lys Lys Phe Tyr Ser 
            900                 905                 910         


Leu Ile Lys Thr Glu Tyr Thr Glu Arg Asp Glu Glu Arg Phe Ile Asn 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn Val Thr Gln Ile 
    930                 935                 940                 


Ile Glu Asp His Tyr Ser Thr Thr Lys Val Ala Ala Ile Arg Ala Asn 
945                 950                 955                 960 


Leu Ser His Glu Phe Arg Val Lys Asn His Ile Tyr Lys Asn Arg Asp 
                965                 970                 975     


Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Ile Val Ala Leu Ile 
            980                 985                 990         


Gly Gly Phe Met Arg Asp Arg Tyr  Pro Asn Met His Asp  Ser Lys Ala 
        995                 1000                 1005             


Val Tyr  Ser Glu Tyr Met Lys  Met Phe Arg Lys Asn  Lys Asn Asp 
    1010                 1015                 1020             


Lys Lys  Arg Trp Lys Asp Gly  Phe Val Ile Asn Ser  Met Asn Tyr 
    1025                 1030                 1035             


Pro Tyr  Glu Val Asp Gly Glu  Leu Ile Trp Asn Pro  Asp Ile Ile 
    1040                 1045                 1050             


Asn Glu  Ile Arg Lys Cys Phe  Tyr Tyr Lys Asp Cys  Tyr Cys Thr 
    1055                 1060                 1065             


Thr Lys  Leu Asp Gln Lys Ser  Gly Gln Met Phe Asn  Leu Thr Val 
    1070                 1075                 1080             


Leu Pro  Asn Asp Ala His Ser  Pro Lys Gly Thr Thr  Glu Ala Val 
    1085                 1090                 1095             


Ile Pro  Val Asn Lys Asn Arg  Lys Asp Val Asn Lys  Tyr Gly Gly 
    1100                 1105                 1110             


Phe Ser  Gly Leu Gln Tyr Val  Ile Val Ala Ile Glu  Gly Lys Lys 
    1115                 1120                 1125             


Lys Arg  Gly Lys Lys Thr Lys  Leu Val Lys Lys Ile  Ser Gly Val 
    1130                 1135                 1140             


Pro Leu  His Leu Lys Ala Ala  Ser Leu Asp Glu Lys  Ile Lys Tyr 
    1145                 1150                 1155             


Ile Glu  Glu Lys Glu Asn Leu  Thr Asp Val Lys Ile  Ile Lys Asp 
    1160                 1165                 1170             


Ser Ile  Pro Val Asn Gln Met  Ile Glu Met Asp Gly  Gly Glu Tyr 
    1175                 1180                 1185             


Leu Leu  Thr Ser Pro Ile Glu  Phe Val Asn Gly Arg  Gln Leu Val 
    1190                 1195                 1200             


Leu Asn  Glu Lys Gln Cys Ala  Leu Ile Ala Asp Ile  Tyr Asn Ala 
    1205                 1210                 1215             


Ile Tyr  Lys Gln Asp Cys Asp  Asn Leu Asp Asp Val  Leu Met Ile 
    1220                 1225                 1230             


Gln Leu  Tyr Ile Glu Leu Ile  Asn Lys Met Lys Ala  Leu Tyr Pro 
    1235                 1240                 1245             


Ala Tyr  Gln Ser Ile Ala Glu  Lys Phe Glu Ser Met  Thr Glu Asp 
    1250                 1255                 1260             


Tyr Val  Ala Val Ser Lys Glu  Glu Lys Ala Asp Ile  Ile Lys Gln 
    1265                 1270                 1275             


Met Leu  Ile Ile Met His Arg  Gly Pro Arg Asn Gly  Lys Ile Gln 
    1280                 1285                 1290             


Tyr Ala  Asp Phe Asn Val Gly  Asp Arg Ile Gly Arg  Lys Asn Lys 
    1295                 1300                 1305             


Met Ser  Leu Asp Leu Glu Arg  Val Thr Phe Val Ser  Gln Ser Pro 
    1310                 1315                 1320             


Thr Gly  Ile Tyr Thr Lys Lys  Tyr Lys Leu 
    1325                 1330             


<210>  837
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  837

Met Gly Lys Glu Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asp Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Cys Asp Thr Asn Tyr Lys Ile Pro Lys Phe Lys Gly 
            20                  25                  30          


Asn Ala Met Trp Gly Ile Arg Leu Phe Glu Lys Ser Asn Ser Ala Glu 
        35                  40                  45              


Glu Arg Arg Gly Phe Arg Ser Ser Arg Arg Arg Thr Gln Arg Lys Arg 
    50                  55                  60                  


Glu Arg Leu Lys Leu Leu Glu Met Leu Phe Asp Glu Glu Ile Ser Lys 
65                  70                  75                  80  


Ile Asp Pro Ala Phe Phe Ile Lys Leu Arg Glu Ser Asn Leu Tyr Leu 
                85                  90                  95      


Glu Asp Lys Ser Thr Lys Ile Pro Tyr Cys Val Phe Ser Asp Glu Asn 
            100                 105                 110         


Tyr Thr Asp Lys Asp Phe His Arg Asp Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Asn Glu Leu Ile Lys Ser Asn Lys Pro His Asp Val Arg Leu Val 
    130                 135                 140                 


Tyr Leu Ala Leu His His Ile Ile Lys Asn Arg Gly His Phe Leu Phe 
145                 150                 155                 160 


Asp Tyr Ser Tyr Lys Asn Asp Lys Ser Gly Asp Phe Met Pro Ile Tyr 
                165                 170                 175     


Asn Asp Leu Lys Asn Tyr Phe Tyr Asp Asn Tyr Glu Ile Glu Leu Asp 
            180                 185                 190         


Cys Ser Asp Val Asp Glu Phe Ala Asn Thr Leu Lys Asp Lys Ser Lys 
        195                 200                 205             


Gly Lys Thr Lys Lys Asn Ala Glu Ile Ala Glu Leu Phe Gly Val Ser 
    210                 215                 220                 


Lys Lys Tyr Asp Lys Val Leu Tyr Ser Ala Leu Ser Leu Leu Ser Gly 
225                 230                 235                 240 


Ser Ser Val Lys Leu Phe Asp Ile Phe Asp Asp Glu Ala Leu Lys Asp 
                245                 250                 255     


Val Glu Lys Lys Ser Val Ser Phe Ser Ser Gly Phe Asp Asp Asn Ala 
            260                 265                 270         


Asn Asp Phe Gln Ser Tyr Leu Gly Glu Arg Phe Glu Leu Leu Glu Lys 
        275                 280                 285             


Leu Lys Ala Val Tyr Asp Trp Ala Val Leu Ala Asp Ile Leu Asp Gly 
    290                 295                 300                 


Lys Glu Tyr Ile Ser Glu Ala Lys Val Ala Ile Tyr Glu Lys His Lys 
305                 310                 315                 320 


Arg Asp Leu Lys Thr Leu Lys Lys Tyr Ile Lys Ile Tyr Met Pro Glu 
                325                 330                 335     


Lys Lys Ala Glu Ile Phe Asn Ile Ser Ser Lys Asp Asn Asn Tyr Val 
            340                 345                 350         


Ala Tyr Ser Lys His Ile Lys Thr Ser Asn Gly Thr Gly Val Leu Ile 
        355                 360                 365             


Ser Thr Cys Thr Gln Glu Glu Phe Cys Ala Tyr Leu Lys Lys Thr Leu 
    370                 375                 380                 


Gly Ile Cys Lys Asp Ala Ser Tyr Glu Glu Met Phe Lys Glu Ile Glu 
385                 390                 395                 400 


Asn Ser Thr Phe Met Pro Lys Gln Val Thr Lys Asp Asn Gly Val Val 
                405                 410                 415     


Pro Met Gln Val Asn Glu Lys Glu Leu Val Ala Ile Leu Asp Asn Ala 
            420                 425                 430         


Lys Glu Tyr Leu Pro Phe Leu Leu Asn Lys Asp Asn Asp Gly Lys Thr 
        435                 440                 445             


Val Tyr Asp Lys Ile Lys Ala Leu Phe Glu Tyr Arg Ile Pro Tyr Tyr 
    450                 455                 460                 


Ile Gly Pro Leu Asn Thr His Ser Thr Lys Ser Trp Leu Val Arg Ser 
465                 470                 475                 480 


Asn Glu Lys Ile Tyr Pro Trp Asn Ile Glu Asp Val Val Asp Phe Asp 
                485                 490                 495     


Lys Ser Ala Glu Lys Phe Ile Glu Asn Leu Thr Ser Lys Cys Thr Tyr 
            500                 505                 510         


Leu Pro Gln Tyr Asp Val Ile Pro Lys His Ser Leu Leu Tyr Asn Lys 
        515                 520                 525             


Phe Cys Val Leu Asn Glu Leu Asn Asn Leu Lys Ile Asp Gly Glu Lys 
    530                 535                 540                 


Ile Ser Val Gln Leu Lys Gln Asp Ile Tyr Asn Asp Leu Phe Lys Lys 
545                 550                 555                 560 


Tyr Tyr Lys Val Thr Asn Lys Lys Leu Cys Asp Tyr Leu Lys Ser Arg 
                565                 570                 575     


Asn Ile Ala Phe Asp Glu Ile Ser Gly Ile Asp Asn Asp Phe Lys Ser 
            580                 585                 590         


Ser Leu Lys Ser Tyr Lys Asp Leu Val Ala Tyr Asn Leu Thr Asp Glu 
        595                 600                 605             


Gln Lys Glu Glu Ile Val Lys Ala Ile Thr Ile Phe Gly Asp Asp Lys 
    610                 615                 620                 


Lys Leu Leu Lys Lys Arg Leu Lys Asn Glu Phe Gly Gly Cys Leu Ser 
625                 630                 635                 640 


Asp Asp Glu Ile Lys Lys Ile Cys Lys Leu Lys Tyr Thr Gly Trp Ser 
                645                 650                 655     


Lys Leu Ser Arg Glu Phe Leu Thr Asp Val Thr Ala Val Tyr Lys Glu 
            660                 665                 670         


Thr Gly Glu Leu Ile Asn Ile Ile Cys Ala Leu Trp Glu Thr Asn Tyr 
        675                 680                 685             


Asn Leu Thr Ser Leu Leu Tyr Ser Asp Glu Phe Cys Cys Asp Asp Lys 
    690                 695                 700                 


Glu Asn Lys Thr Phe Asn Asp Lys Ile Asn Glu Leu Asn Ser Phe Gly 
705                 710                 715                 720 


Asn Asp Lys Thr Leu Asn Gln Ile Val Glu Glu Leu Tyr Val Ser Pro 
                725                 730                 735     


Leu Val Lys Arg Pro Ile Tyr Gln Ser Leu Leu Ile Thr Lys Glu Leu 
            740                 745                 750         


Glu Lys Ile Met Lys Cys Pro Pro Lys Lys Ile Phe Val Glu Val Ala 
        755                 760                 765             


Arg Gly Ala Glu Glu Lys Lys Arg Thr Val Ser Arg Arg Asn Lys Leu 
    770                 775                 780                 


Leu Asp Leu Tyr Lys Ser Cys Lys Asn Asp Tyr Pro Leu Leu Phe Asp 
785                 790                 795                 800 


Glu Leu Gln Asn Asn Thr Thr Asp Asp Glu Leu Lys Ser Asp Lys Leu 
                805                 810                 815     


Tyr Leu Tyr Phe Thr Gln Phe Gly Lys Cys Met Tyr Thr Gly Glu Asn 
            820                 825                 830         


Ile Glu Leu Gly Asp Leu Phe Asn Lys Asn Leu Tyr Asp Ile Asp His 
        835                 840                 845             


Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Leu Asp Asn Arg Val 
    850                 855                 860                 


Leu Val Lys Lys Thr Thr Asn Ala Lys Lys Asp Asn Glu Tyr Pro Leu 
865                 870                 875                 880 


Ser Ser Gln Ile Arg Glu Lys Met Thr Pro His Trp Lys Ala Leu Leu 
                885                 890                 895     


Asp Lys Gly Leu Ile Asn Lys Lys Lys Tyr Glu Arg Leu Val Arg Asn 
            900                 905                 910         


Tyr Pro Leu Ser Asp Glu Glu Leu Ser Gln Phe Ile Ser Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ser Thr Lys Ala Val Ala Asn Ile Leu Glu Gln 
    930                 935                 940                 


Leu Tyr Pro Arg Pro Asp Thr Glu Ile Val Tyr Val Lys Ala Ala Leu 
945                 950                 955                 960 


Ala Ser Glu Phe Arg His Gln Tyr Asp Met Leu Lys Cys Arg Glu Val 
                965                 970                 975     


Asn Asp Leu His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         


Asn Ala Tyr Asn Gly Lys Phe Thr  His Asn Lys Ala Asn  Phe Ile Lys 
        995                 1000                 1005             


Gly Leu  Gln Asn Asn Asp Lys  Ala Tyr Thr Val Lys  Leu Asp Ser 
    1010                 1015                 1020             


Met Leu  Lys His Asn Ile Asp  Gly Ala Trp Ile Ala  Asp Asn Asn 
    1025                 1030                 1035             


Glu Ser  Leu Asn Ile Val Lys  Ser Thr Met Asn Lys  Asn Asn Ile 
    1040                 1045                 1050             


Arg Tyr  Thr Arg Tyr Ala Phe  Glu Lys Lys Gly Gly  Leu Phe Lys 
    1055                 1060                 1065             


Val Thr  Ile Leu Lys Lys Gly  Asn Gly Gln Ala Ser  Ile Lys Lys 
    1070                 1075                 1080             


Asn Asp  Lys Arg Asn Ser Ile  Glu Lys Tyr Gly Gly  Tyr Asn Gln 
    1085                 1090                 1095             


Val Ser  Ser Thr Tyr Phe Ser  Cys Val Glu His Gly  Lys Asp Asn 
    1100                 1105                 1110             


Asn Arg  Met Ile Ser Met Phe  Ser Val Asn Leu Tyr  Asp Lys Asn 
    1115                 1120                 1125             


Lys Tyr  Glu Glu Asn Pro Ile  Ala Tyr Leu Ile Glu  Asn Tyr Gly 
    1130                 1135                 1140             


Leu Ile  Asn Pro Ile Ile Lys  Val Lys Lys Val Lys  Ile Gln Ser 
    1145                 1150                 1155             


Cys Ile  Ser Phe Asp Asn Met  Leu Cys His Leu Gly  Gly Lys Asp 
    1160                 1165                 1170             


Ser Gly  Gly Ser Gln Ile Ser  Tyr Arg Pro Gly Val  Gln Leu Val 
    1175                 1180                 1185             


Leu Gly  Tyr Asp Asn Glu Arg  Tyr Ile Arg Asn Val  Ile Arg Cys 
    1190                 1195                 1200             


Ala Gly  Gly Asp Leu His Leu  Gln Thr Ser Lys Asn  Glu Lys Asp 
    1205                 1210                 1215             


Ile Val  Thr Ala Glu Lys Asn  Ile Glu Leu Phe Asn  Leu Leu Ile 
    1220                 1225                 1230             


Glu Lys  Met Lys Asn Asn Ile  Tyr Gly Val Leu Tyr  Ser Asn Leu 
    1235                 1240                 1245             


Gly Asp  Lys Leu Tyr Cys Ser  Lys Glu Lys Phe Glu  Lys Leu Asp 
    1250                 1255                 1260             


Ile Val  Glu Gln Cys Asn Val  Ile Lys Asn Val Leu  Thr Ile Leu 
    1265                 1270                 1275             


Lys Asn  Asn Ala Ser Lys Gly  Asp Leu Lys Ser Ile  Gly Ile Lys 
    1280                 1285                 1290             


Ala Asn  Gly Ala Ile Arg Leu  Asn Asn Asn Ile Ser  Glu Ile Lys 
    1295                 1300                 1305             


Lys Val  Lys Ser Ile Lys Leu  Ile Asn Gln Ser Val  Thr Gly Leu 
    1310                 1315                 1320             


Phe Glu  Gln Glu Ile Glu Leu  Leu Asn Leu 
    1325                 1330             


<210>  838
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  838

Met Ser Gln Asn Ile Val Asp Tyr Cys Ile Gly Leu Asp Leu Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Val Asp Met Asn His Arg Leu Met Lys 
            20                  25                  30          


Arg Asn Gly Lys His Leu Trp Gly Ser Arg Leu Phe Ser Asn Ala Glu 
        35                  40                  45              


Thr Ala Ala Thr Arg Arg Ser Ser Arg Ser Ile Arg Arg Arg Tyr Asn 
    50                  55                  60                  


Lys Arg Arg Glu Arg Ile Arg Leu Leu Arg Ala Ile Leu Gln Asp Met 
65                  70                  75                  80  


Val Leu Glu Lys Asp Pro Thr Phe Phe Ile Arg Leu Glu His Thr Ser 
                85                  90                  95      


Phe Leu Asp Glu Glu Asp Lys Ala Lys Tyr Leu Gly Thr Asp Tyr Lys 
            100                 105                 110         


Asp Asn Tyr Asn Leu Phe Ile Asp Glu Asp Phe Asn Asp Tyr Thr Tyr 
        115                 120                 125             


Tyr His Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Ala Leu Cys Glu 
    130                 135                 140                 


Ser Thr Glu Lys Ala Asp Pro Arg Leu Ile Tyr Leu Ala Leu His His 
145                 150                 155                 160 


Ile Val Lys Tyr Arg Gly Asn Phe Leu Tyr Glu Gly Gln Lys Phe Asn 
                165                 170                 175     


Met Asp Ala Ser Asn Ile Glu Asp Lys Leu Ser Asp Val Phe Thr Gln 
            180                 185                 190         


Phe Ala Asp Phe Asn Asn Ile Pro Tyr Glu Asp Asp Glu Lys Lys Asn 
        195                 200                 205             


Leu Glu Ile Leu Glu Ile Leu Lys Lys Pro Leu Ser Lys Lys Ala Lys 
    210                 215                 220                 


Val Asp Glu Val Met Ala Leu Ile Ser Pro Glu Lys Glu Phe Lys Ser 
225                 230                 235                 240 


Ala Tyr Lys Glu Leu Val Thr Gly Ile Ala Gly Asn Lys Met Asn Val 
                245                 250                 255     


Thr Lys Met Ile Leu Cys Glu Ser Ile Lys Gln Gly Asp Ser Glu Ile 
            260                 265                 270         


Lys Leu Lys Phe Ser Asp Ser Asn Tyr Asp Asp Gln Phe Ser Glu Val 
        275                 280                 285             


Glu Asn Asp Leu Gly Glu Tyr Val Glu Phe Ile Asp Ser Leu His Asn 
    290                 295                 300                 


Ile Tyr Ser Trp Val Glu Leu Gln Thr Ile Met Gly Ala Thr His Thr 
305                 310                 315                 320 


Asp Asn Ala Ser Ile Ser Glu Ala Met Val Ser Arg Tyr Asn Lys His 
                325                 330                 335     


His Glu Asp Leu Gln Leu Leu Lys Lys Cys Ile Lys Asp Asn Val Pro 
            340                 345                 350         


Lys Lys Tyr Phe Asp Met Phe Arg Asn Asp Ser Glu Lys Val Lys Gly 
        355                 360                 365             


Tyr Tyr Asn Tyr Ile Asn Arg Pro Asn Lys Ala Pro Val Asp Glu Phe 
    370                 375                 380                 


Tyr Lys Phe Val Lys Lys Cys Ile Glu Lys Val Asp Thr Pro Glu Ala 
385                 390                 395                 400 


Lys Gln Ile Leu His Asp Ile Glu Leu Glu Asn Phe Leu Leu Lys Gln 
                405                 410                 415     


Asn Ser Arg Thr Asn Gly Ser Val Pro Tyr Gln Met Gln Leu Asp Glu 
            420                 425                 430         


Met Ile Lys Ile Ile Asp Asn Gln Ala Lys Tyr Tyr Pro Val Leu Lys 
        435                 440                 445             


Glu Lys Arg Glu Gln Leu Leu Ser Ile Leu Thr Phe Arg Ile Pro Tyr 
    450                 455                 460                 


Tyr Phe Gly Pro Leu Asn Glu Thr Ser Glu His Ala Trp Ile Lys Arg 
465                 470                 475                 480 


Leu Glu Gly Lys Glu Asn Gln Arg Ile Leu Pro Trp Asn Tyr Gln Asp 
                485                 490                 495     


Thr Val Asp Val Asp Ala Thr Ala Glu Gly Phe Ile Lys Arg Met Arg 
            500                 505                 510         


Ser Tyr Cys Thr Tyr Phe Pro Asp Glu Glu Val Leu Pro Lys Asn Ser 
        515                 520                 525             


Leu Ile Val Ser Lys Tyr Glu Val Tyr Asn Glu Leu Asn Lys Ile Arg 
    530                 535                 540                 


Val Asp Asp Lys Leu Leu Glu Val Asp Val Lys Asn Asp Ile Tyr Asn 
545                 550                 555                 560 


Glu Leu Phe Met Lys Asn Lys Thr Val Thr Glu Lys Lys Leu Lys Asn 
                565                 570                 575     


Trp Leu Val Asn Asn Gln Cys Cys Arg Lys Asp Ala Glu Ile Lys Gly 
            580                 585                 590         


Phe Gln Lys Glu Asn Gln Phe Ser Thr Ser Leu Thr Pro Trp Ile Asp 
        595                 600                 605             


Phe Thr Asn Ile Phe Gly Lys Ile Asp Gln Ser Asn Phe Asp Leu Ile 
    610                 615                 620                 


Glu Lys Ile Ile Tyr Asp Leu Thr Val Phe Glu Asp Lys Lys Ile Met 
625                 630                 635                 640 


Lys Arg Arg Leu Lys Lys Lys Tyr Ala Leu Pro Asp Asp Lys Ile Lys 
                645                 650                 655     


Gln Ile Leu Lys Leu Lys Tyr Lys Asp Trp Ser Arg Leu Ser Lys Lys 
            660                 665                 670         


Leu Leu Asp Gly Ile Val Ala Asp Asn Arg Phe Gly Ser Ser Val Thr 
        675                 680                 685             


Val Leu Asp Val Leu Glu Met Ser Arg Leu Asn Leu Met Glu Ile Ile 
    690                 695                 700                 


Asn Asp Lys Glu Leu Gly Tyr Ala Gln Met Ile Glu Glu Ala Ser Ser 
705                 710                 715                 720 


Cys Pro Lys Asp Gly Lys Phe Thr Tyr Glu Glu Val Ala Lys Leu Ala 
                725                 730                 735     


Gly Ser Pro Ala Leu Lys Arg Gly Ile Trp Gln Ser Leu Gln Ile Val 
            740                 745                 750         


Glu Glu Ile Thr Lys Val Met Lys Cys Arg Pro Lys Tyr Ile Tyr Ile 
        755                 760                 765             


Glu Phe Glu Arg Ser Glu Glu Ala Lys Glu Arg Thr Glu Ser Lys Ile 
    770                 775                 780                 


Lys Lys Leu Glu Asn Val Tyr Lys Asp Leu Asp Glu Gln Thr Lys Lys 
785                 790                 795                 800 


Glu Tyr Lys Ser Val Leu Glu Glu Leu Lys Gly Phe Asp Asn Thr Lys 
                805                 810                 815     


Lys Ile Ser Ser Asp Ser Leu Phe Leu Tyr Phe Thr Gln Leu Gly Lys 
            820                 825                 830         


Cys Met Tyr Ser Gly Lys Lys Leu Asp Ile Asp Ser Leu Asp Lys Tyr 
        835                 840                 845             


Gln Ile Asp His Ile Val Pro Gln Ser Leu Val Lys Asp Asp Ser Phe 
    850                 855                 860                 


Asp Asn Arg Val Leu Val Leu Pro Ser Glu Asn Gln Arg Lys Leu Asp 
865                 870                 875                 880 


Asp Leu Val Val Pro Phe Asp Ile Arg Asp Lys Met Tyr Arg Phe Trp 
                885                 890                 895     


Lys Leu Leu Phe Asp His Glu Leu Ile Ser Pro Lys Lys Phe Tyr Ser 
            900                 905                 910         


Leu Ile Lys Thr Glu Tyr Thr Glu Arg Asp Glu Glu Arg Phe Ile Asn 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn Val Thr Gln Ile 
    930                 935                 940                 


Ile Glu Asp His Tyr Ser Thr Thr Lys Val Ala Ala Ile Arg Ala Asn 
945                 950                 955                 960 


Leu Ser His Glu Phe Arg Val Lys Asn His Ile Tyr Lys Asn Arg Asp 
                965                 970                 975     


Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Ile Val Ala Leu Ile 
            980                 985                 990         


Gly Gly Phe Met Arg Asp Arg Tyr  Pro Asn Met His Asp  Ser Lys Ala 
        995                 1000                 1005             


Val Tyr  Ser Glu Tyr Met Lys  Met Phe Arg Lys Asn  Lys Asn Asp 
    1010                 1015                 1020             


Gln Lys  Lys Trp Lys Asp Gly  Phe Val Ile Asn Ser  Met Asn Tyr 
    1025                 1030                 1035             


Pro Tyr  Glu Val Asp Gly Lys  Leu Ile Trp Asn Pro  Asp Leu Ile 
    1040                 1045                 1050             


Asn Glu  Ile Lys Lys Cys Phe  Tyr Tyr Lys Asp Cys  Tyr Cys Thr 
    1055                 1060                 1065             


Thr Lys  Leu Asp Gln Lys Ser  Gly Gln Met Phe Asn  Leu Thr Val 
    1070                 1075                 1080             


Leu Pro  Asn Asp Ala His Ser  Ala Lys Gly Thr Thr  Glu Ala Val 
    1085                 1090                 1095             


Ile Pro  Val Asn Lys Asn Arg  Lys Asp Val Asn Lys  Tyr Gly Gly 
    1100                 1105                 1110             


Phe Ser  Gly Leu Gln Tyr Val  Ile Ala Ala Ile Glu  Gly Thr Lys 
    1115                 1120                 1125             


Lys Lys  Gly Lys Lys Leu Val  Lys Val Arg Lys Leu  Ser Gly Ile 
    1130                 1135                 1140             


Pro Leu  Tyr Leu Lys Gln Ala  Asp Ile Lys Glu Gln  Ile Glu Tyr 
    1145                 1150                 1155             


Val Glu  Lys Glu Glu Lys Leu  Ser Asp Val Lys Ile  Ile Lys Asn 
    1160                 1165                 1170             


Asn Ile  Pro Leu Asn Gln Leu  Ile Glu Ile Asp Gly  Arg Gln Tyr 
    1175                 1180                 1185             


Leu Leu  Thr Ser Pro Thr Glu  Cys Val Asn Ala Met  Gln Leu Val 
    1190                 1195                 1200             


Leu Asn  Glu Glu Gln Cys Lys  Leu Ile Ala Asp Ile  Tyr Asn Ala 
    1205                 1210                 1215             


Ile Tyr  Lys Gln Asp Phe Asp  Gly Leu Asp Asn Met  Leu Met Ile 
    1220                 1225                 1230             


Gln Leu  Tyr Leu Gln Leu Ile  Asp Lys Leu Lys Thr  Leu Tyr Pro 
    1235                 1240                 1245             


Ile Tyr  Met Gly Ile Val Glu  Lys Phe Glu Lys Leu  Thr Glu Asp 
    1250                 1255                 1260             


Phe Val  Ser Ile Ser Lys Glu  Glu Lys Ala Asn Val  Ile Lys Gln 
    1265                 1270                 1275             


Met Leu  Ile Ile Met His Lys  Gly Pro Gln Asn Gly  Asn Ile Ile 
    1280                 1285                 1290             


Tyr Asp  Asp Phe Asn Val Gly  Lys Arg Ile Gly Arg  Leu Asn Gly 
    1295                 1300                 1305             


Arg Thr  Phe Tyr Leu Asp Asn  Ile Glu Phe Ile Ser  Gln Ser Pro 
    1310                 1315                 1320             


Thr Gly  Ile Tyr Thr Lys Lys  Tyr Lys Leu 
    1325                 1330             


<210>  839
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  839

Met Tyr Thr Tyr Tyr Phe Lys Glu Ser Glu Met Val Tyr Asp Val Gly 
1               5                   10                  15      


Leu Asp Ile Gly Thr Gly Ser Val Gly Trp Val Ala Leu Asp Glu Asn 
            20                  25                  30          


Gly Lys Leu Ala Arg Ala Lys Gly Lys Asn Leu Val Gly Val Arg Leu 
        35                  40                  45              


Phe Asp Thr Ala Gln Thr Ala Ala Asp Arg Arg Gly Phe Arg Thr Thr 
    50                  55                  60                  


Arg Arg Arg Leu Ser Arg Arg Lys Trp Arg Leu Arg Leu Leu Asp Glu 
65                  70                  75                  80  


Leu Phe Ser Ala Glu Ile Asn Glu Ile Asp Ser Ser Phe Phe Gln Arg 
                85                  90                  95      


Leu Lys Tyr Ser Tyr Val His Pro Lys Asp Glu Glu Asn Lys Ala His 
            100                 105                 110         


Tyr Tyr Gly Gly Tyr Leu Phe Pro Thr Glu Glu Glu Thr Lys Lys Phe 
        115                 120                 125             


His Arg Ser Tyr Pro Thr Ile Tyr His Leu Arg Gln Glu Leu Met Ala 
    130                 135                 140                 


Gln Pro Asn Lys Arg Phe Asp Ile Arg Glu Ile Tyr Leu Ala Ile His 
145                 150                 155                 160 


His Leu Val Lys Tyr Arg Gly His Phe Leu Ser Ser Gln Glu Lys Ile 
                165                 170                 175     


Thr Ile Gly Ser Thr Tyr Asn Pro Glu Asp Leu Ala Asn Ala Ile Glu 
            180                 185                 190         


Val Tyr Ala Asp Glu Lys Gly Leu Ser Trp Glu Leu Asn Asn Pro Glu 
        195                 200                 205             


Gln Leu Thr Glu Ile Ile Ser Gly Glu Ala Gly Tyr Gly Leu Asn Lys 
    210                 215                 220                 


Ser Met Lys Ala Asp Glu Ala Leu Lys Leu Phe Glu Phe Asp Asn Asn 
225                 230                 235                 240 


Gln Asp Lys Val Ala Ile Lys Thr Leu Leu Ala Gly Leu Thr Gly Asn 
                245                 250                 255     


Gln Ile Asp Phe Ala Lys Leu Phe Gly Lys Asp Ile Ser Asp Lys Asp 
            260                 265                 270         


Glu Ala Lys Leu Trp Lys Leu Lys Leu Asp Asp Glu Ala Leu Glu Glu 
        275                 280                 285             


Lys Ser Gln Thr Ile Leu Ser Gln Leu Thr Asp Glu Glu Ile Glu Leu 
    290                 295                 300                 


Phe His Ala Val Val Gln Ala Tyr Asp Gly Phe Val Leu Ile Gly Leu 
305                 310                 315                 320 


Leu Asn Gly Ala Asp Ser Val Ser Ala Ala Met Val Gln Leu Tyr Asp 
                325                 330                 335     


Gln His Arg Glu Asp Arg Lys Leu Leu Lys Ser Leu Ala Gln Lys Ala 
            340                 345                 350         


Gly Leu Lys His Lys Arg Phe Ser Glu Ile Tyr Glu Gln Leu Ala Leu 
        355                 360                 365             


Ala Thr Asp Glu Ala Thr Ile Lys Asn Gly Ile Ser Thr Ala Arg Glu 
    370                 375                 380                 


Leu Val Glu Glu Ser Asn Leu Ser Lys Glu Val Lys Glu Asp Thr Leu 
385                 390                 395                 400 


Arg Arg Leu Asp Glu Asn Glu Phe Leu Pro Lys Gln Arg Thr Lys Ala 
                405                 410                 415     


Asn Ser Val Ile Pro His Gln Leu His Leu Ala Glu Leu Gln Lys Ile 
            420                 425                 430         


Leu Gln Asn Gln Gly Gln Tyr Tyr Pro Phe Leu Leu Asp Thr Phe Glu 
        435                 440                 445             


Lys Glu Asp Gly Gln Asp Asn Lys Ile Glu Glu Leu Leu Arg Phe Arg 
    450                 455                 460                 


Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Lys Lys Asp Val Glu His 
465                 470                 475                 480 


Ala Gly Gly Asp Ala Asp Asn His Trp Val Glu Arg Asn Glu Gly Phe 
                485                 490                 495     


Glu Lys Ser Arg Val Thr Pro Trp Asn Phe Asp Lys Val Phe Asn Arg 
            500                 505                 510         


Asp Lys Ala Ala Arg Asp Phe Ile Glu Arg Leu Thr Gly Asn Asp Thr 
        515                 520                 525             


Tyr Leu Ile Gly Glu Lys Thr Leu Pro Gln Asn Ser Leu Arg Tyr Gln 
    530                 535                 540                 


Leu Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Gly Lys 
545                 550                 555                 560 


Lys Phe Asp Ser Lys Thr Lys Ala Asp Leu Ile Asn Asp Leu Phe Lys 
                565                 570                 575     


Ala Arg Lys Thr Val Ser Leu Ser Ala Leu Lys Asp Tyr Leu Lys Ala 
            580                 585                 590         


Gln Gly Lys Gly Asp Val Thr Ile Thr Gly Leu Ala Asp Glu Ser Lys 
        595                 600                 605             


Phe Asn Ser Ser Leu Ser Ser Tyr Asn Asp Leu Lys Lys Thr Phe Asp 
    610                 615                 620                 


Ala Glu Tyr Leu Glu Asn Glu Asp Asn Gln Glu Thr Leu Glu Lys Ile 
625                 630                 635                 640 


Ile Glu Ile Gln Thr Val Phe Glu Asp Ser Lys Ile Ala Ser Arg Glu 
                645                 650                 655     


Leu Ser Lys Leu Pro Leu Asp Asp Asp Gln Val Lys Lys Leu Ser Gln 
            660                 665                 670         


Thr His Tyr Thr Gly Trp Gly Arg Leu Ser Glu Lys Leu Leu Asp Ser 
        675                 680                 685             


Lys Ile Ile Asp Glu Arg Gly Gln Lys Val Ser Ile Leu Asp Lys Leu 
    690                 695                 700                 


Lys Ser Thr Ser Gln Asn Phe Met Ser Ile Ile Asn Asn Asp Lys Tyr 
705                 710                 715                 720 


Gly Val Gln Ala Trp Ile Thr Glu Gln Asn Thr Gly Ser Ser Lys Leu 
                725                 730                 735     


Thr Phe Asp Glu Lys Val Asn Glu Leu Thr Thr Ser Pro Ala Asn Lys 
            740                 745                 750         


Arg Gly Ile Lys Gln Ser Phe Ala Val Leu Asn Asp Ile Lys Lys Ala 
        755                 760                 765             


Met Lys Glu Glu Pro Arg Arg Val Tyr Leu Glu Phe Ala Arg Glu Asp 
    770                 775                 780                 


Gln Thr Ser Val Arg Ser Val Pro Arg Tyr Asn Gln Leu Lys Glu Lys 
785                 790                 795                 800 


Tyr Gln Ser Lys Ser Leu Ser Glu Glu Ala Lys Val Leu Lys Lys Thr 
                805                 810                 815     


Leu Asp Gly Asn Lys Asn Lys Met Ser Asp Asp Arg Tyr Phe Leu Tyr 
            820                 825                 830         


Phe Gln Gln Gln Gly Lys Asp Met Tyr Thr Gly Arg Pro Ile Asn Phe 
        835                 840                 845             


Glu Arg Leu Ser Gln Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala 
    850                 855                 860                 


Phe Thr Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Pro 
865                 870                 875                 880 


Glu Asn Ala Arg Lys Ser Asp Ser Phe Ala Tyr Thr Asp Glu Val Gln 
                885                 890                 895     


Lys Gln Asp Gly Ser Leu Trp Thr Ser Leu Leu Lys Ser Gly Phe Ile 
            900                 905                 910         


Asn Arg Lys Lys Tyr Glu Arg Leu Thr Lys Ala Gly Lys Tyr Leu Asp 
        915                 920                 925             


Gly Gln Lys Thr Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Ile Ile Lys Asn Val Ala Ser Leu Ile Glu Gly Glu Tyr Glu Asn Ser 
945                 950                 955                 960 


Lys Ala Val Ala Ile Arg Ser Glu Ile Thr Ala Asp Met Arg Leu Leu 
                965                 970                 975     


Val Gly Ile Lys Lys His Arg Glu Ile Asn Ser Phe His His Ala Phe 
            980                 985                 990         


Asp Ala Leu Leu Ile Thr Ala Ala  Gly Gln Tyr Met Gln  Asn Arg Tyr 
        995                 1000                 1005             


Pro Asp  Arg Asp Ser Thr Asn  Val Tyr Asn Glu Phe  Asp Arg Tyr 
    1010                 1015                 1020             


Thr Asn  Asp Tyr Leu Lys Asn  Leu Arg Gln Leu Ser  Ser Arg Asp 
    1025                 1030                 1035             


Glu Val  Arg Arg Leu Lys Ser  Phe Gly Phe Val Val  Gly Thr Met 
    1040                 1045                 1050             


Arg Lys  Gly Asn Glu Asp Trp  Ser Glu Glu Asn Thr  Ser Tyr Leu 
    1055                 1060                 1065             


Arg Lys  Val Met Met Phe Lys  Asn Ile Leu Thr Thr  Lys Lys Thr 
    1070                 1075                 1080             


Glu Lys  Asp Arg Gly Pro Leu  Asn Lys Glu Thr Ile  Phe Ser Pro 
    1085                 1090                 1095             


Lys Ser  Gly Lys Lys Leu Ile  Pro Leu Asn Ser Lys  Arg Ser Asp 
    1100                 1105                 1110             


Thr Ala  Leu Tyr Gly Gly Tyr  Ser Asn Val Tyr Ser  Ala Tyr Met 
    1115                 1120                 1125             


Thr Leu  Val Arg Ala Asn Gly  Lys Asn Leu Leu Ile  Lys Ile Pro 
    1130                 1135                 1140             


Ile Ser  Ile Ala Asn Gln Ile  Glu Val Gly Asn Leu  Lys Ile Asn 
    1145                 1150                 1155             


Asp Tyr  Ile Val Asn Asn Pro  Ala Ile Lys Lys Phe  Glu Lys Ile 
    1160                 1165                 1170             


Leu Ile  Ser Lys Leu Pro Leu  Gly Gln Leu Val Asn  Glu Asp Gly 
    1175                 1180                 1185             


Asn Leu  Ile Tyr Leu Ala Ser  Asn Glu Tyr Arg His  Asn Ala Lys 
    1190                 1195                 1200             


Gln Leu  Trp Leu Ser Thr Thr  Asp Ala Asp Lys Ile  Ala Ser Ile 
    1205                 1210                 1215             


Ser Glu  Asn Ser Ser Asp Glu  Glu Leu Leu Glu Ala  Tyr Asp Ile 
    1220                 1225                 1230             


Leu Thr  Ser Glu Asn Val Lys  Asn Arg Phe Pro Phe  Phe Lys Lys 
    1235                 1240                 1245             


Asp Ile  Asp Lys Leu Ser Gln  Val Arg Asp Glu Phe  Leu Asp Ser 
    1250                 1255                 1260             


Asp Lys  Arg Ile Ala Val Ile  Gln Thr Ile Leu Arg  Gly Leu Gln 
    1265                 1270                 1275             


Ile Asp  Ala Ala Tyr Gln Ala  Pro Val Lys Ile Ile  Ser Lys Lys 
    1280                 1285                 1290             


Val Ser  Asp Trp His Lys Leu  Gln Gln Ser Gly Gly  Ile Lys Leu 
    1295                 1300                 1305             


Ser Asp  Asn Ser Glu Met Ile  Tyr Gln Ser Ala Thr  Gly Ile Phe 
    1310                 1315                 1320             


Glu Thr  Arg Val Lys Ile Ser  Asp Leu Leu 
    1325                 1330             


<210>  840
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  840

Met Lys Asn Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asn Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Ser Asp Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Asp Gly Gln Thr Ala Val Asp Arg Arg Met 
    50                  55                  60                  


Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Val Glu Met Ala Asn Ile Asp Ala Asn 
                85                  90                  95      


Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Lys Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     


Lys Asn Thr Ser Val Asp Gly Val Tyr Glu Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Met Ser Asn Ile Glu Glu Gly Thr Leu Ala Lys Val 
        195                 200                 205             


Glu Glu Asn Ile Glu Val Ala Asn Ile Leu Ala Gly Lys Phe Thr Arg 
    210                 215                 220                 


Arg Glu Lys Phe Glu Arg Ile Leu Gln Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 


Thr Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Ser Lys Gly 
                245                 250                 255     


Asn Phe Gln Lys Val Phe Asp Leu Ile Glu Lys Thr Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Thr Tyr Asn 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Thr Ala Thr Glu Thr Asn 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Ala His Glu Lys 
                325                 330                 335     


Asp Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys Gln 
            340                 345                 350         


Tyr Gln Glu Ile Phe Asn Asn Ala Ala Ile Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Lys Gln Val Asp Phe Tyr Lys Tyr Leu Lys Thr 
    370                 375                 380                 


Ile Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu 
385                 390                 395                 400 


Glu Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Ile His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Arg Glu Asp Tyr Glu Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Lys Gly 
    450                 455                 460                 


Gln Ser Glu Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Val Arg Tyr Ile Asp Asp Gln Gly Lys Thr Asn Tyr 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln 
545                 550                 555                 560 


Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Arg Asn Ile 
                565                 570                 575     


Asn His Ile Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         


Ala Ser Tyr Ala Thr Tyr His Asp Leu Leu Lys Val Gly Met Lys Gln 
        595                 600                 605             


Glu Ile Leu Asp Asn Pro Leu Asn Thr Glu Met Leu Glu Asp Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Pro Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Gln Gln Phe Ser Asp Val Leu Asp Gly Gly Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Val 
            660                 665                 670         


Gly Ile Arg Glu Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Thr 
705                 710                 715                 720 


Asp Lys Asp Leu Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Ser Ile Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Tyr 
    770                 775                 780                 


Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Lys Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Glu Leu Lys Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 


Gly Asn Arg Glu Lys Gly Gly Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Asp Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Asn 
    930                 935                 940                 


Glu Thr Asp Asn His Gly Asn Thr Met Glu Gln Val Arg Ile Val Thr 
945                 950                 955                 960 


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Glu  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Gly Gln Lys Glu  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Glu Thr Ile Lys  Lys Val Leu 
    1055                 1060                 1065             


Asp Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Lys Ala Thr  Ile Lys Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Glu  Asn Trp Asp Pro Met  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Leu  Asp Ser Pro Asn Met  Ala Tyr Ala Val Ile  Ile Glu His 
    1115                 1120                 1125             


Ala Lys  Gly Lys Lys Lys Leu  Ile Phe Glu Lys Lys  Ile Ile Arg 
    1130                 1135                 1140             


Ile Thr  Ile Met Glu Arg Lys  Met Phe Glu Lys Asp  Glu Glu Ala 
    1145                 1150                 1155             


Phe Leu  Glu Glu Lys Gly Tyr  Arg His Pro Lys Val  Leu Thr Lys 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Lys Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Ala Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Leu Val 
    1190                 1195                 1200             


Leu Ser  Asn His Leu Val Ser  Leu Leu Tyr His Ala  Lys Asn Cys 
    1205                 1210                 1215             


Glu Ala  Ser Asp Gly Lys Ser  Leu Lys Tyr Ile Glu  Ala His Arg 
    1220                 1225                 1230             


Glu Thr  Phe Ser Glu Leu Leu  Ala Gln Val Ser Glu  Phe Ala Thr 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Asp Ala  Asn Leu Ser Lys Ile  Asn Asn Leu 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Glu Gly  Asp Ile Gln Ala Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asp Leu Met Ala Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Phe Phe Glu Ala Thr  Ile Asp Arg Lys Arg  Tyr Thr Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Ser Ser  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ser Arg Lys  Arg Leu Asp Asp 
    1325                 1330                 


<210>  841
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  841

Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Ala Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Leu Trp 
        35                  40                  45              


Gly Val Arg Leu Val Asp Glu Gly Lys Thr Ala Ala His Arg Arg Val 
    50                  55                  60                  


Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Thr Ala Glu Met Phe Glu Val Asp Ala Asn 
                85                  90                  95      


Phe Phe Tyr Arg Leu Glu Asp Ser Phe Tyr Ile Glu Ser Glu Lys Arg 
            100                 105                 110         


Gln Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Glu Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 


Ser Ser Asp Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asp Thr 
                165                 170                 175     


Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Lys Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Ala Ser Asp Ile Glu Glu Gly Ser Leu Thr Arg Ile 
        195                 200                 205             


Glu Glu Asn Asn Glu Val Ala Lys Ile Phe Ser Glu Lys Leu Thr Lys 
    210                 215                 220                 


Arg Glu Lys Leu Asp Lys Ile Leu Asn Leu Tyr Pro Asn Glu Lys Ser 
225                 230                 235                 240 


Thr Asp Leu Phe Ala Gln Phe Ile Ser Leu Ile Ile Gly Ser Lys Gly 
                245                 250                 255     


Asn Phe Lys Lys Phe Phe Asn Leu Thr Glu Lys Thr Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Val Leu Leu Ala Arg Val 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Ile Phe Val Ala Ala Lys Asn Ala Tyr Asn 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Ser Asn Thr Glu Thr Lys 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Lys His Asp Lys 
                325                 330                 335     


Asp Leu Lys Arg Met Lys Ala Phe Phe Lys Val Arg Leu Pro Glu Asn 
            340                 345                 350         


Phe Asn Glu Val Phe Asn Asp Val Glu Lys Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Glu Gly Lys Thr Lys Gln Glu Ala Phe Tyr Lys Tyr Met Lys Lys 
    370                 375                 380                 


Met Leu Glu His Val Glu Gly Ala Asp Tyr Phe Ile Asn Gln Ile Glu 
385                 390                 395                 400 


Glu Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Lys Val Asp Tyr Glu Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly 
    450                 455                 460                 


Gln Ser Glu Phe Ser Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Ile Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Met Cys Tyr Gln Lys Tyr Met Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Ile Arg Tyr Thr Asp Asp Gln Gly Lys Thr His Tyr 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln 
545                 550                 555                 560 


Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Tyr Asn Met 
                565                 570                 575     


Asn His Val Glu Ser Pro Thr Val Glu Gly Val Glu Asp Ala Phe Asn 
            580                 585                 590         


Ser Ser Phe Thr Thr Tyr His Asp Leu Gln Lys Val Gly Val Pro Gln 
        595                 600                 605             


Glu Ile Leu Asp Asp Pro Leu Asn Thr Glu Met Leu Glu Glu Ile Ile 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Asn Glu Arg Leu 
625                 630                 635                 640 


Gln Glu Phe Ser Asn Val Leu Asp Glu Ala Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile 
            660                 665                 670         


Gly Ile Arg Asp Lys Glu Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Lys His Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala 
705                 710                 715                 720 


Asp Lys Asp Ile Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Gly Ile Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe 
    770                 775                 780                 


Thr Ser Leu Glu Lys Ala Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Gly Leu Lys Asn Asp Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Val Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Arg Val Leu Ala Ser Ser Ala 
    850                 855                 860                 


Ala Asn Arg Glu Lys Gly Asp Asn Val Pro Ser Leu Glu Val Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Tyr Trp Glu Lys Leu Tyr Gln Ala Lys Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Glu Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Cys 
    930                 935                 940                 


Lys Lys Asp Glu Ser Gly Asn Val Ile Glu Gln Val Arg Ile Val Thr 
945                 950                 955                 960 


Leu Lys Ala Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Cys Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Ala Lys Lys Asp  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Asp Thr Ile Lys  Lys Val Leu 
    1055                 1060                 1065             


Asn Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Asn Ala Thr  Ala Asn Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Ala  Asp Trp Asp Pro Ile  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Phe  Asp Gly Ser Asn Met  Ala Tyr Ala Ile Val  Ile Glu His 
    1115                 1120                 1125             


Glu Lys  Arg Lys Lys Lys Thr  Val Ile Lys Lys Glu  Leu Ile Gln 
    1130                 1135                 1140             


Ile Asn  Ile Met Glu Arg Thr  Ala Phe Glu Lys Asp  Gln Lys Glu 
    1145                 1150                 1155             


Phe Leu  Glu Gly Lys Gly Tyr  Arg Asn Pro Lys Val  Ile Thr Lys 
    1160                 1165                 1170             


Ile Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Asn Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Gly Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Met Val 
    1190                 1195                 1200             


Leu Pro  Asn His Leu Met Thr  Leu Leu Tyr His Ala  Lys Asn Cys 
    1205                 1210                 1215             


Glu Ala  Ser Asp Gly Lys Ser  Leu Ala Tyr Ile Glu  Ser His Arg 
    1220                 1225                 1230             


Glu Met  Phe Ala Glu Leu Leu  Asp Ser Ile Ser Glu  Phe Ala Ser 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Asp Ala  Asn Leu Glu Lys Ile  Asn Thr Ile 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Ser Gly  Asp Val Lys Val Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asn Leu Leu Glu Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Tyr Phe Glu Thr Asn  Ile Glu Arg Lys Arg  Tyr Asn Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Asn Ala  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ala Arg Lys  Arg Leu Asp Asp 
    1325                 1330                 


<210>  842
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  842

Met Lys Asn Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Ser Asp Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Asp Gly Gln Thr Ala Val Asp Arg Arg Met 
    50                  55                  60                  


Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Val Glu Met Ala Asn Ile Asp Ala Asn 
                85                  90                  95      


Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Lys Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     


Lys Asn Thr Ser Val Asp Glu Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Met Ser Asn Ile Glu Glu Gly Ala Leu Ala Lys Val 
        195                 200                 205             


Glu Glu Asn Thr Glu Val Ala Ser Ile Leu Ala Gly Lys Phe Thr Arg 
    210                 215                 220                 


Arg Glu Lys Phe Glu Arg Ile Leu Arg Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 


Thr Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Asn Lys Gly 
                245                 250                 255     


Asn Phe Gln Lys Val Phe Asn Leu Val Glu Lys Thr Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Thr Tyr Asn 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Thr Ala Thr Glu Thr Asn 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Ala His Glu Lys 
                325                 330                 335     


Glu Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys Gln 
            340                 345                 350         


Tyr Gln Glu Ile Phe Asn Asn Ala Glu Ile Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Lys Gln Val Asp Phe Tyr Lys Tyr Leu Lys Thr 
    370                 375                 380                 


Thr Leu Glu Asn Val Glu Gly Ala Asp Tyr Phe Ile Thr Lys Ile Glu 
385                 390                 395                 400 


Glu Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Val Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Arg Glu Gly Tyr Asp Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly 
    450                 455                 460                 


Gln Ser Glu Phe Ala Trp Leu Thr Arg Lys Asp Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Ile Arg Tyr Ile Asp Asp Gln Gly Lys Thr Asn Tyr 
    530                 535                 540                 


Phe Ser Gly Arg Glu Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln 
545                 550                 555                 560 


Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Arg Asn Ile 
                565                 570                 575     


Asn His Ile Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         


Ala Ser Tyr Ala Thr Tyr His Asp Leu Leu Lys Val Gly Leu Lys Gln 
        595                 600                 605             


Glu Ile Leu Asp Asn Pro Leu Asn Thr Glu Ile Leu Glu Asp Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Glu Gln Phe Ser Asp Val Leu Asp Gly Val Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Val 
            660                 665                 670         


Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Thr 
705                 710                 715                 720 


Asp Lys Asp Leu Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Ser Ile Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Tyr 
    770                 775                 780                 


Lys Ser Leu Glu Lys Ala Ile Lys Asp Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Glu Leu Lys Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Ile Tyr Thr Gly Gln Glu Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 


Gly Asn Arg Glu Lys Gly Gly Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Glu Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Lys 
    930                 935                 940                 


Glu Thr Asp Asn His Gly Asn Thr Met Glu Gln Val Arg Ile Val Thr 
945                 950                 955                 960 


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Glu  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Ala Gln Lys Glu  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Glu Thr Ile Lys  Lys Val Leu 
    1055                 1060                 1065             


Asp Tyr  Arg Gln Ile Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Lys Ala Thr  Ile Lys Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Glu  Asn Trp Asp Pro Val  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Leu  Asp Ser Pro Asn Met  Ala Tyr Ala Val Ile  Ile Glu His 
    1115                 1120                 1125             


Ala Lys  Gly Lys Lys Lys Ile  Val Ile Glu Lys Lys  Leu Ile Gln 
    1130                 1135                 1140             


Ile Asn  Ile Met Glu Arg Lys  Met Phe Glu Lys Asp  Glu Glu Ala 
    1145                 1150                 1155             


Phe Leu  Glu Glu Lys Gly Tyr  His Gln Pro Lys Val  Leu Thr Lys 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Lys Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Ser Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Leu Val 
    1190                 1195                 1200             


Leu Ser  Asn His Leu Val Ser  Leu Leu Tyr His Ala  Lys Asn Cys 
    1205                 1210                 1215             


Glu Ala  Ser Asp Gly Lys Ser  Leu Lys Tyr Ile Glu  Ala His Arg 
    1220                 1225                 1230             


Glu Thr  Phe Ser Glu Leu Leu  Ala Gln Val Ser Glu  Phe Ala Thr 
    1235                 1240                 1245             


Lys Tyr  Thr Leu Ala Asp Ala  Asn Leu Ser Lys Ile  Asn Asn Leu 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Glu Gly  Asp Ile Lys Ala Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asp Leu Met Ala Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Phe Phe Glu Ala Thr  Ile Asp Arg Lys Arg  Tyr Thr Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Ser Ser  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ser Arg Lys  Arg Leu Asp Asp 
    1325                 1330                 


<210>  843
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  843

Met Gly Arg Lys Pro Tyr Ile Leu Ser Leu Asp Ile Gly Thr Gly Ser 
1               5                   10                  15      


Val Gly Tyr Ala Cys Met Asp Lys Gly Phe Asn Val Leu Lys Tyr His 
            20                  25                  30          


Asp Lys Asp Ala Leu Gly Val Tyr Leu Phe Asp Gly Ala Leu Thr Ala 
        35                  40                  45              


Gln Glu Arg Arg Gln Phe Arg Thr Ser Arg Arg Arg Lys Asn Arg Arg 
    50                  55                  60                  


Ile Lys Arg Leu Gly Leu Leu Gln Glu Leu Leu Ala Pro Leu Val Gln 
65                  70                  75                  80  


Asn Pro Asn Phe Tyr Gln Phe Gln Arg Gln Phe Ala Trp Lys Asn Asp 
                85                  90                  95      


Asn Met Asp Phe Lys Asn Lys Ser Leu Ser Glu Val Leu Ser Phe Leu 
            100                 105                 110         


Gly Tyr Glu Ser Lys Lys Tyr Pro Thr Ile Tyr His Leu Gln Glu Ala 
        115                 120                 125             


Leu Leu Leu Lys Asp Glu Lys Phe Asp Pro Glu Leu Ile Tyr Met Ala 
    130                 135                 140                 


Leu Tyr His Leu Val Lys Tyr Arg Gly His Phe Leu Phe Asp His Leu 
145                 150                 155                 160 


Lys Ile Glu Asn Leu Thr Asn Asn Asp Asn Met His Asp Phe Val Glu 
                165                 170                 175     


Leu Ile Glu Thr Tyr Glu Asn Leu Asn Asn Ile Lys Leu Asn Leu Asp 
            180                 185                 190         


Tyr Glu Lys Thr Lys Val Ile Tyr Glu Ile Leu Lys Asp Asn Glu Met 
        195                 200                 205             


Thr Lys Asn Asp Arg Ala Lys Arg Val Lys Asn Met Gln Lys Lys Leu 
    210                 215                 220                 


Glu Gln Phe Ser Ile Met Leu Leu Gly Leu Lys Phe Asn Glu Gly Lys 
225                 230                 235                 240 


Leu Phe Asn His Ala Asp Asn Ala Glu Glu Leu Lys Gly Ala Asn Gln 
                245                 250                 255     


Ser His Thr Phe Ala Asp Asn Tyr Glu Glu Asn Leu Thr Pro Phe Leu 
            260                 265                 270         


Thr Val Glu Gln Ser Glu Phe Ile Glu Arg Ala Asn Lys Ile Tyr Leu 
        275                 280                 285             


Ser Leu Thr Leu Gln Asp Ile Leu Lys Gly Lys Lys Ser Met Ala Met 
    290                 295                 300                 


Ser Lys Val Ala Ala Tyr Asp Lys Phe Arg Asn Glu Leu Lys Gln Val 
305                 310                 315                 320 


Lys Asp Ile Val Tyr Lys Ala Asp Ser Thr Arg Thr Gln Phe Lys Lys 
                325                 330                 335     


Ile Phe Val Ser Ser Lys Lys Ser Leu Lys Gln Tyr Asp Ala Thr Pro 
            340                 345                 350         


Asn Asp Gln Thr Phe Ser Ser Leu Cys Leu Phe Asp Gln Tyr Leu Ile 
        355                 360                 365             


Arg Pro Lys Lys Gln Tyr Ser Leu Leu Ile Lys Glu Leu Lys Lys Ile 
    370                 375                 380                 


Ile Pro Gln Asp Ser Glu Leu Tyr Phe Glu Ala Glu Asn Asp Thr Leu 
385                 390                 395                 400 


Leu Lys Val Leu Asn Thr Thr Asp Asn Ala Ser Ile Pro Met Gln Ile 
                405                 410                 415     


Asn Leu Tyr Glu Ala Glu Thr Ile Leu Arg Asn Gln Gln Lys Tyr His 
            420                 425                 430         


Ala Glu Ile Thr Asp Glu Met Ile Glu Lys Val Leu Ser Leu Ile Gln 
        435                 440                 445             


Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Asn Asp His Thr Ala 
    450                 455                 460                 


Ser Lys Phe Gly Trp Met Glu Arg Lys Ser Asn Glu Ser Ile Lys Pro 
465                 470                 475                 480 


Trp Asn Phe Asp Glu Val Val Asp Arg Ser Lys Ser Ala Thr Gln Phe 
                485                 490                 495     


Ile Arg Arg Met Thr Asn Lys Cys Ser Tyr Leu Ile Asn Glu Asp Val 
            500                 505                 510         


Leu Pro Lys Asn Ser Leu Leu Tyr Gln Glu Met Glu Val Leu Asn Glu 
        515                 520                 525             


Leu Asn Ala Thr Gln Ile Arg Leu Gln Thr Asp Pro Lys Asn Arg Lys 
    530                 535                 540                 


Tyr Arg Met Met Pro Gln Ile Lys Leu Phe Ala Val Glu His Ile Phe 
545                 550                 555                 560 


Lys Lys Tyr Lys Thr Val Ser His Ser Lys Phe Leu Glu Ile Met Leu 
                565                 570                 575     


Asn Ser Asn His Arg Glu Asn Phe Met Asn His Gly Glu Lys Leu Ser 
            580                 585                 590         


Ile Phe Gly Thr Gln Asp Asp Lys Lys Phe Ala Ser Lys Leu Ser Ser 
        595                 600                 605             


Tyr Gln Asp Met Thr Lys Ile Phe Gly Asp Ile Glu Gly Lys Arg Ala 
    610                 615                 620                 


Gln Ile Glu Glu Ile Ile Gln Trp Ile Thr Ile Phe Glu Asp Lys Lys 
625                 630                 635                 640 


Ile Leu Val Gln Lys Leu Lys Glu Cys Tyr Pro Glu Leu Thr Ser Lys 
                645                 650                 655     


Gln Ile Asn Gln Leu Lys Lys Leu Asn Tyr Ser Gly Trp Gly Arg Leu 
            660                 665                 670         


Ser Glu Lys Leu Leu Thr His Ala Tyr Gln Gly His Ser Ile Ile Glu 
        675                 680                 685             


Leu Leu Arg His Ser Asp Glu Asn Phe Met Glu Ile Leu Thr Asn Asp 
    690                 695                 700                 


Val Tyr Gly Phe Gln Asn Phe Ile Lys Glu Glu Asn Gln Val Gln Ser 
705                 710                 715                 720 


Asn Lys Ile Gln His Gln Asp Ile Ala Asn Leu Thr Thr Ser Pro Ala 
                725                 730                 735     


Leu Lys Lys Gly Ile Trp Ser Thr Ile Lys Leu Val Arg Glu Leu Thr 
            740                 745                 750         


Ser Ile Phe Gly Glu Pro Glu Lys Ile Ile Met Glu Phe Ala Thr Glu 
        755                 760                 765             


Asp Gln Gln Lys Gly Lys Glu Gln Lys Ser Arg Lys Gln Leu Trp Asp 
    770                 775                 780                 


Asp Asn Ile Lys Lys Asn Lys Leu Lys Ser Val Asp Glu Tyr Lys Tyr 
785                 790                 795                 800 


Ile Ile Asp Val Ala Asn Lys Leu Asn Asn Glu Gln Leu Gln Gln Glu 
                805                 810                 815     


Lys Leu Trp Leu Tyr Leu Ser Gln Asn Gly Lys Cys Met Tyr Ser Gly 
            820                 825                 830         


Gln Ser Ile Asp Leu Asp Ala Leu Leu Ser Pro Asn Ala Thr Lys His 
        835                 840                 845             


Tyr Glu Val Asp His Ile Phe Pro Arg Ser Phe Ile Lys Asp Asp Ser 
    850                 855                 860                 


Ile Asp Asn Lys Val Leu Val Ile Lys Lys Met Asn Gln Thr Lys Gly 
865                 870                 875                 880 


Asp Gln Val Pro Leu Gln Phe Ile Gln Gln Pro Tyr Glu Arg Ile Ala 
                885                 890                 895     


Tyr Trp Lys Ser Leu Asn Lys Ala Gly Leu Ile Ser Asp Ser Lys Leu 
            900                 905                 910         


His Lys Leu Met Lys Pro Glu Phe Thr Ala Met Asp Lys Glu Gly Phe 
        915                 920                 925             


Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Ser Val His Val Arg 
    930                 935                 940                 


Asp Phe Leu Lys Glu Glu Tyr Pro Asn Thr Lys Val Ile Pro Met Lys 
945                 950                 955                 960 


Ala Lys Met Val Ser Glu Phe Arg Lys Lys Phe Asp Ile Pro Lys Ile 
                965                 970                 975     


Arg Gln Met Asn Asp Ala His His Ala Ile Asp Ala Tyr Leu Asn Gly 
            980                 985                 990         


Val Val Tyr His Gly Ala Gln Leu  Ala Tyr Pro Asn Val  Asp Leu Phe 
        995                 1000                 1005             


Asp Phe  Asn Phe Lys Trp Glu  Lys Val Arg Glu Lys  Trp Lys Ala 
    1010                 1015                 1020             


Leu Gly  Glu Phe Asn Thr Lys  Gln Lys Ser Arg Glu  Leu Phe Phe 
    1025                 1030                 1035             


Phe Lys  Lys Leu Glu Lys Met  Glu Val Ser Gln Gly  Glu Arg Leu 
    1040                 1045                 1050             


Ile Ser  Lys Ile Lys Leu Asp  Met Asn His Phe Lys  Ile Asn Tyr 
    1055                 1060                 1065             


Ser Arg  Lys Leu Ala Asn Ile  Pro Gln Gln Phe Tyr  Asn Gln Thr 
    1070                 1075                 1080             


Ala Val  Ser Pro Lys Thr Ala  Glu Leu Lys Tyr Glu  Ser Asn Lys 
    1085                 1090                 1095             


Ser Asn  Glu Val Val Tyr Lys  Gly Leu Thr Pro Tyr  Gln Thr Tyr 
    1100                 1105                 1110             


Val Val  Ala Ile Lys Ser Val  Asn Lys Lys Gly Lys  Glu Lys Met 
    1115                 1120                 1125             


Glu Tyr  Gln Met Ile Asp His  Tyr Val Phe Asp Phe  Tyr Lys Phe 
    1130                 1135                 1140             


Gln Asn  Gly Asn Glu Lys Glu  Leu Ala Leu Tyr Leu  Ala Gln Arg 
    1145                 1150                 1155             


Glu Asn  Lys Asp Glu Val Leu  Asp Ala Gln Ile Val  Tyr Ser Leu 
    1160                 1165                 1170             


Asn Lys  Gly Asp Leu Leu Tyr  Ile Asn Asn His Pro  Cys Tyr Phe 
    1175                 1180                 1185             


Val Ser  Arg Lys Glu Val Ile  Asn Ala Lys Gln Phe  Glu Leu Thr 
    1190                 1195                 1200             


Val Glu  Gln Gln Leu Ser Leu  Tyr Asn Val Met Asn  Asn Lys Glu 
    1205                 1210                 1215             


Thr Asn  Val Glu Lys Leu Leu  Ile Glu Tyr Asp Phe  Ile Ala Glu 
    1220                 1225                 1230             


Lys Val  Ile Asn Glu Tyr His  His Tyr Leu Asn Ser  Lys Leu Lys 
    1235                 1240                 1245             


Glu Lys  Arg Val Arg Thr Phe  Phe Ser Glu Ser Asn  Gln Thr His 
    1250                 1255                 1260             


Glu Asp  Phe Ile Lys Ala Leu  Asp Glu Leu Phe Lys  Val Val Thr 
    1265                 1270                 1275             


Ala Ser  Ala Thr Arg Ser Asp  Lys Ile Gly Ser Arg  Lys Asn Ser 
    1280                 1285                 1290             


Met Thr  His Arg Ala Phe Leu  Gly Lys Gly Lys Asp  Val Lys Ile 
    1295                 1300                 1305             


Ala Tyr  Thr Ser Ile Ser Gly  Leu Lys Thr Thr Lys  Pro Lys Ser 
    1310                 1315                 1320             


Leu Phe  Lys Leu Ala Glu Ser  Arg Asn Glu Leu 
    1325                 1330                 


<210>  844
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  844

Met Lys Asn Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Ser Asp Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  


Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Leu Glu Met Ala Asn Ile Asp Ala Asn 
                85                  90                  95      


Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Lys Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     


Lys Asn Thr Ser Val Asp Gly Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Ile Ser Asn Ile Glu Glu Gly Thr Leu Ala Lys Met 
        195                 200                 205             


Glu Glu Asn Thr Thr Val Ala Asp Ile Leu Ala Gly Lys Phe Thr Arg 
    210                 215                 220                 


Lys Glu Lys Leu Glu Arg Ile Leu Gln Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 


Thr Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Ser Lys Gly 
                245                 250                 255     


Asn Phe Gln Lys Val Phe Asp Leu Val Glu Lys Thr Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Thr Tyr Asn 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Thr Asp Thr Glu Thr Asn 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Ala His Glu Lys 
                325                 330                 335     


Asp Leu Arg Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys Gln 
            340                 345                 350         


Tyr Glu Glu Ile Phe Ser Asn Val Ala Ile Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Lys Gln Val Asp Phe Tyr Lys Tyr Leu Lys Thr 
    370                 375                 380                 


Leu Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu 
385                 390                 395                 400 


Glu Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Phe Lys Glu Ala Tyr Asp Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly 
    450                 455                 460                 


Gln Ser Asp Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Tyr Tyr Gln Lys Tyr Met Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Val Arg Tyr Ile Asp Asp Gln Gly Lys Thr Asn Tyr 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Gln Gln Ile Phe Asn Asp Tyr Phe Lys Lys 
545                 550                 555                 560 


Lys Arg Lys Val Ser Lys Lys Asp Leu Glu Gln Phe Leu Arg Asn Met 
                565                 570                 575     


Ser His Ile Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         


Ser Ser Tyr Ala Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln 
        595                 600                 605             


Glu Val Leu Glu Asn Pro Leu Asn Thr Glu Met Leu Glu Asp Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Gln Gln Phe Ser Asp Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Val 
            660                 665                 670         


Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Thr 
705                 710                 715                 720 


Asp Lys Asp Leu Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Ser Ile Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Val Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr 
    770                 775                 780                 


Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 


Gly Asn Arg Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Phe Trp Glu Lys Leu Phe Gln Gly Asn Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Glu Ala Asp Lys Ala Thr Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Asn 
    930                 935                 940                 


Glu Thr Asp Asn His Gly Asn Asn Met Glu Gln Val Arg Ile Val Met 
945                 950                 955                 960 


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Glu  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Ala Gln Lys Glu  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Glu Thr Ile Lys  Lys Val Leu 
    1055                 1060                 1065             


Asp Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Lys Ala Thr  Ile Lys Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Glu  Asn Trp Asp Pro Met  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Leu  Asp Ser Pro Asn Met  Ala Tyr Ala Val Ile  Ile Glu His 
    1115                 1120                 1125             


Ala Lys  Gly Lys Lys Lys Val  Val Phe Glu Lys Lys  Ile Ile Arg 
    1130                 1135                 1140             


Ile Thr  Ile Met Glu Arg Lys  Ala Phe Glu Lys Asp  Glu Lys Ser 
    1145                 1150                 1155             


Phe Leu  Glu Lys Gln Gly Tyr  Arg Gln Pro Lys Val  Leu Thr Lys 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Asn Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Ala Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Gln Val 
    1190                 1195                 1200             


Leu Lys  Gly Gln Leu Ile Thr  Leu Leu His His Ala  Lys Asn Cys 
    1205                 1210                 1215             


Glu Ala  Ser Asp Gly Lys Ser  Leu Asp Tyr Ile Glu  Ser Asn Arg 
    1220                 1225                 1230             


Glu Met  Phe Gly Glu Leu Leu  Ala His Val Ser Glu  Phe Ala Lys 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Asp Ala  Asn Leu Ser Lys Ile  Asn Gln Leu 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Asp Asn  Asp Ile Lys Val Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asn Leu Met Ala Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Phe Phe Glu Ala Thr  Ile Glu Arg Lys Arg  Tyr Thr Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Ser Ala  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ala Arg Lys  Arg Leu Asp Gly 
    1325                 1330                 


<210>  845
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  845

Met Lys Asn Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asn Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Ser Asp Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Asp Gly Gln Thr Ala Val Asp Arg Arg Met 
    50                  55                  60                  


Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Val Glu Met Ala Asn Ile Asp Ala Asn 
                85                  90                  95      


Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Asp Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     


Lys Asn Thr Ser Val Asp Glu Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Met Ser Asn Ile Glu Glu Gly Ala Leu Ala Lys Val 
        195                 200                 205             


Glu Glu Asn Leu Glu Val Ala Ser Ile Leu Ala Gly Lys Phe Thr Arg 
    210                 215                 220                 


Arg Glu Lys Phe Glu Arg Ile Leu Arg Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 


Thr Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Asn Lys Gly 
                245                 250                 255     


Asn Phe Gln Lys Val Phe Asn Leu Val Glu Lys Thr Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Thr Tyr Asn 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Thr Ala Thr Glu Thr Asn 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Ala His Glu Lys 
                325                 330                 335     


Asp Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys Gln 
            340                 345                 350         


Tyr Gln Glu Ile Phe Asn Asn Ala Ala Ile Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Lys Gln Val Asp Phe Tyr Lys Tyr Leu Lys Thr 
    370                 375                 380                 


Thr Leu Glu Asn Val Glu Gly Ala Asp Tyr Phe Ile Thr Lys Ile Glu 
385                 390                 395                 400 


Glu Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Val Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Arg Glu Asp Tyr Glu Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Lys Gly 
    450                 455                 460                 


Gln Ser Glu Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Val Arg Tyr Ile Asp Asp Gln Gly Lys Thr Asn Tyr 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln 
545                 550                 555                 560 


Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Arg Asn Ile 
                565                 570                 575     


Asn His Ile Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         


Ala Ser Tyr Ala Thr Tyr His Asp Leu Leu Lys Val Gly Met Lys Gln 
        595                 600                 605             


Glu Ser Leu Asp Ser Pro Leu Asn Ala Glu Met Leu Glu Asp Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Gln Gln Phe Ser Asp Val Leu Asp Gly Val Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Val 
            660                 665                 670         


Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Thr 
705                 710                 715                 720 


Asp Lys Asp Leu Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Ser Ile Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Val Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr 
    770                 775                 780                 


Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Glu Leu Lys Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Ile Tyr Thr Gly Gln Glu Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 


Gly Asn Arg Glu Lys Gly Gly Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Asp Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Asn 
    930                 935                 940                 


Glu Thr Asp Asn Tyr Gly Asn Thr Met Glu Gln Val Arg Ile Val Thr 
945                 950                 955                 960 


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Glu  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Ala Gln Lys Glu  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Glu Thr Ile Lys  Lys Val Leu 
    1055                 1060                 1065             


Asp Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Lys Ala Thr  Ile Lys Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Glu  Asn Trp Asp Pro Met  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Leu  Asp Ser Pro Asn Met  Ala Tyr Ala Val Ile  Ile Glu His 
    1115                 1120                 1125             


Ala Lys  Gly Lys Lys Lys Ile  Val Ile Glu Lys Lys  Leu Ile Gln 
    1130                 1135                 1140             


Ile Asn  Ile Met Glu Arg Lys  Met Phe Glu Lys Asp  Glu Glu Ala 
    1145                 1150                 1155             


Phe Leu  Glu Glu Lys Gly Tyr  Arg His Pro Lys Val  Leu Thr Lys 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Lys Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Ala Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Leu Val 
    1190                 1195                 1200             


Leu Ser  Asn His Leu Val Ser  Leu Leu Tyr His Ala  Lys Asn Cys 
    1205                 1210                 1215             


Glu Ala  Ser Asp Gly Lys Ser  Leu Lys Tyr Ile Glu  Ala His Arg 
    1220                 1225                 1230             


Glu Ala  Phe Ser Glu Leu Leu  Ala Gln Val Ser Glu  Phe Ala Thr 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Asp Ala  Asn Leu Ser Lys Ile  Asn Asn Leu 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Glu Gly  Asp Ile Lys Ala Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asp Leu Met Val Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Phe Phe Glu Ala Thr  Ile Asp Arg Lys Arg  Tyr Thr Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Ser Ser  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ser Arg Lys  Arg Leu Asp Asp 
    1325                 1330                 


<210>  846
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  846

Met Lys Asn Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Ser Asp Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  


Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Leu Glu Met Ala Asn Ile Asp Ala Asn 
                85                  90                  95      


Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Lys Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     


Lys Asn Thr Ser Val Asp Gly Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Ile Ser Asn Ile Glu Glu Gly Thr Leu Ala Lys Met 
        195                 200                 205             


Glu Glu Asn Thr Thr Val Ala Asn Ile Leu Ala Gly Lys Phe Thr Arg 
    210                 215                 220                 


Lys Glu Lys Leu Glu Arg Ile Leu Gln Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 


Thr Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Ser Lys Gly 
                245                 250                 255     


Asn Phe Gln Lys Val Phe Asp Leu Val Glu Lys Thr Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Thr Tyr Asn 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Thr Asp Thr Glu Thr Asn 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Ala His Glu Lys 
                325                 330                 335     


Asp Leu Ser Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys Gln 
            340                 345                 350         


Tyr Glu Glu Ile Phe Ser Asn Val Ala Ile Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Lys Gln Val Asp Phe Tyr Lys Tyr Leu Lys Thr 
    370                 375                 380                 


Leu Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu 
385                 390                 395                 400 


Glu Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Lys Glu Ala Tyr Asp Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly 
    450                 455                 460                 


Gln Ser Asp Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Tyr Tyr Gln Lys Tyr Met Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Val Arg Tyr Ile Asp Asp Gln Gly Lys Thr Asn Tyr 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Gln Gln Ile Phe Asn Asp Tyr Phe Lys Gln 
545                 550                 555                 560 


Lys Arg Lys Val Ser Lys Lys Asp Leu Glu Gln Phe Leu Arg Asn Met 
                565                 570                 575     


Ser His Ile Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         


Ser Ser Tyr Ala Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln 
        595                 600                 605             


Glu Val Leu Glu Asn Pro Leu Asn Met Glu Met Leu Glu Asp Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Gln Gln Phe Ser Gly Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Val 
            660                 665                 670         


Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Thr 
705                 710                 715                 720 


Asp Lys Asp Leu Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Ser Ile Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Val Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr 
    770                 775                 780                 


Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 


Gly Asn Arg Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Phe Trp Glu Lys Leu Phe Gln Gly Asn Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Glu Ala Asp Lys Ala Thr Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Asn 
    930                 935                 940                 


Glu Thr Asp Asn His Gly Asn Asn Met Glu Gln Val Arg Ile Val Met 
945                 950                 955                 960 


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Glu  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Ala Gln Lys Glu  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Glu Thr Ile Lys  Lys Val Leu 
    1055                 1060                 1065             


Asp Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Lys Ala Thr  Ile Lys Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Glu  Asn Trp Asp Pro Met  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Leu  Asp Ser Pro Asn Met  Ala Tyr Ala Val Ile  Ile Glu His 
    1115                 1120                 1125             


Ala Lys  Gly Lys Lys Lys Ile  Val Phe Glu Lys Lys  Ile Ile Arg 
    1130                 1135                 1140             


Ile Thr  Ile Met Glu Arg Lys  Ala Phe Glu Lys Asp  Glu Lys Ser 
    1145                 1150                 1155             


Phe Leu  Glu Lys Gln Gly Tyr  Arg Gln Pro Lys Val  Leu Thr Lys 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Asn Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Ala Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Gln Val 
    1190                 1195                 1200             


Leu Lys  Gly Gln Leu Ile Thr  Leu Leu His His Ala  Lys Asn Cys 
    1205                 1210                 1215             


Glu Ala  Ser Asp Gly Lys Ser  Leu Asp Tyr Ile Glu  Ser Asn Arg 
    1220                 1225                 1230             


Glu Met  Phe Gly Glu Leu Leu  Ala His Val Ser Glu  Phe Ala Lys 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Asp Ala  Asn Leu Ser Lys Ile  Asn Gln Leu 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Asp Asn  Asp Ile Lys Val Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asn Leu Met Ala Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Phe Phe Glu Ala Thr  Ile Glu Arg Lys Arg  Tyr Thr Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Arg Ala  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ala Arg Lys  Arg Leu Asp Gly 
    1325                 1330                 


<210>  847
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  847

Met Lys Asn Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Ser Asp Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  


Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Leu Glu Met Ala Asn Ile Asp Ala Asn 
                85                  90                  95      


Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Lys Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     


Lys Asn Thr Ser Val Asp Gly Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Ile Ser Asn Ile Glu Glu Gly Thr Leu Ala Lys Met 
        195                 200                 205             


Glu Glu Asn Thr Thr Val Ala Asp Ile Leu Ala Gly Lys Phe Thr Arg 
    210                 215                 220                 


Lys Glu Lys Leu Glu Arg Ile Leu Gln Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 


Thr Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Ser Lys Gly 
                245                 250                 255     


Asn Phe Gln Lys Val Phe Asp Leu Val Glu Lys Thr Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Thr Tyr Asn 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Thr Asp Thr Glu Thr Asn 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Ala His Glu Lys 
                325                 330                 335     


Asp Leu Ser Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys Gln 
            340                 345                 350         


Tyr Glu Glu Ile Phe Ser Asn Val Ala Ile Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Lys Gln Val Asp Phe Tyr Lys Tyr Leu Lys Thr 
    370                 375                 380                 


Leu Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu 
385                 390                 395                 400 


Glu Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Lys Glu Ala Tyr Asp Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly 
    450                 455                 460                 


Gln Ser Asp Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Tyr Tyr Gln Lys Tyr Met Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Val Arg Tyr Ile Asp Asp Gln Gly Lys Thr Asn Tyr 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Gln Gln Ile Phe Asn Asp Tyr Phe Lys Gln 
545                 550                 555                 560 


Lys Arg Lys Val Ser Lys Lys Asp Leu Glu Gln Phe Leu Arg Asn Met 
                565                 570                 575     


Ser His Ile Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         


Ser Ser Tyr Ala Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln 
        595                 600                 605             


Glu Val Leu Glu Asn Pro Leu Asn Thr Glu Met Leu Glu Asp Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Gln Gln Phe Ser Asp Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Val 
            660                 665                 670         


Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Thr 
705                 710                 715                 720 


Asp Lys Asp Leu Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Ser Ile Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Val Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr 
    770                 775                 780                 


Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 


Gly Asn Arg Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Phe Trp Glu Lys Leu Phe Gln Gly Asn Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Glu Ala Asp Lys Ala Thr Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Asn 
    930                 935                 940                 


Glu Thr Asp Asn His Gly Asn Asn Met Glu Gln Val Arg Ile Val Met 
945                 950                 955                 960 


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Glu  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Ala Gln Lys Glu  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Glu Thr Ile Lys  Lys Val Leu 
    1055                 1060                 1065             


Asp Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Lys Ala Thr  Ile Lys Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Glu  Asn Trp Asp Pro Met  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Leu  Asp Ser Pro Asn Met  Ala Tyr Ala Val Ile  Ile Glu His 
    1115                 1120                 1125             


Ala Lys  Gly Lys Lys Lys Val  Val Phe Glu Lys Lys  Ile Ile Arg 
    1130                 1135                 1140             


Ile Thr  Ile Met Glu Arg Lys  Ala Phe Glu Lys Asp  Glu Lys Ser 
    1145                 1150                 1155             


Phe Leu  Glu Lys Gln Gly Tyr  Arg Gln Pro Lys Val  Leu Thr Lys 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Asn Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Ala Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Gln Val 
    1190                 1195                 1200             


Leu Lys  Gly Gln Leu Ile Thr  Leu Leu His His Ala  Lys Asn Cys 
    1205                 1210                 1215             


Glu Ala  Ser Asp Gly Lys Ser  Leu Asp Tyr Ile Glu  Ser Asn Arg 
    1220                 1225                 1230             


Glu Met  Phe Gly Glu Leu Leu  Ala His Val Ser Glu  Phe Ala Lys 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Asp Ala  Asn Leu Ser Lys Ile  Asn Gln Leu 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Asp Asn  Asp Ile Lys Val Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asn Leu Met Ala Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Phe Phe Glu Ala Thr  Ile Glu Arg Lys Arg  Tyr Thr Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Ser Ala  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ala Arg Lys  Arg Leu Asp Gly 
    1325                 1330                 


<210>  848
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  848

Met Lys Asn Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asn Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Ser Asp Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Asp Gly Gln Thr Ala Val Asp Arg Arg Met 
    50                  55                  60                  


Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Val Glu Met Ala Asn Ile Asp Ala Asn 
                85                  90                  95      


Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Asp Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     


Lys Asn Thr Ser Val Asp Glu Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Met Ser Asn Ile Glu Glu Gly Ala Leu Ala Lys Val 
        195                 200                 205             


Glu Glu Asn Leu Glu Val Ala Ser Ile Leu Ala Gly Lys Phe Thr Arg 
    210                 215                 220                 


Arg Glu Lys Phe Glu Arg Ile Leu Arg Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 


Thr Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Asn Lys Gly 
                245                 250                 255     


Asn Phe Gln Lys Val Phe Asn Leu Val Glu Lys Thr Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Thr Tyr Asn 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Thr Ala Thr Glu Thr Asn 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Ala His Glu Lys 
                325                 330                 335     


Asp Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys Gln 
            340                 345                 350         


Tyr Gln Glu Ile Phe Asn Asn Ala Ala Ile Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Lys Gln Val Asp Phe Tyr Lys Tyr Leu Lys Thr 
    370                 375                 380                 


Thr Leu Glu Asn Val Glu Gly Ala Asp Tyr Phe Ile Thr Lys Ile Glu 
385                 390                 395                 400 


Glu Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Val Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Arg Glu Asp Tyr Glu Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Lys Gly 
    450                 455                 460                 


Gln Ser Glu Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Val Arg Tyr Ile Asp Asp Gln Gly Lys Thr Asn Tyr 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln 
545                 550                 555                 560 


Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Arg Asn Ile 
                565                 570                 575     


Asn His Ile Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         


Ala Ser Tyr Ala Thr Tyr His Asp Leu Leu Lys Val Gly Met Lys Gln 
        595                 600                 605             


Glu Ser Leu Asp Ser Pro Leu Asn Ala Glu Met Leu Glu Asp Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Gln Gln Phe Ser Asp Val Leu Asp Gly Val Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Val 
            660                 665                 670         


Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Thr 
705                 710                 715                 720 


Asp Lys Asp Leu Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Ser Ile Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Val Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr 
    770                 775                 780                 


Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Glu Leu Lys Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Ile Tyr Thr Gly Gln Glu Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 


Gly Asn Arg Glu Lys Gly Gly Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Asp Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Thr Asn Ile Leu His Gln Arg Phe Asn Asn 
    930                 935                 940                 


Glu Thr Asp Asn Tyr Gly Asn Thr Met Glu Gln Val Arg Ile Val Thr 
945                 950                 955                 960 


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Glu  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Ala Gln Lys Glu  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Glu Thr Ile Lys  Lys Val Leu 
    1055                 1060                 1065             


Asp Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Lys Ala Thr  Ile Lys Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Glu  Asn Trp Asp Pro Met  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Leu  Asp Ser Pro Asn Met  Ala Tyr Ala Val Ile  Ile Glu His 
    1115                 1120                 1125             


Ala Lys  Gly Lys Lys Lys Ile  Val Ile Glu Lys Lys  Leu Ile Gln 
    1130                 1135                 1140             


Ile Asn  Ile Met Glu Arg Lys  Met Phe Glu Lys Asp  Glu Glu Ala 
    1145                 1150                 1155             


Phe Leu  Glu Glu Lys Gly Tyr  Arg His Pro Lys Val  Leu Thr Lys 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Lys Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Ala Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Leu Val 
    1190                 1195                 1200             


Leu Ser  Asn His Leu Val Ser  Leu Leu Tyr His Ala  Lys Asn Cys 
    1205                 1210                 1215             


Glu Ala  Ser Asp Gly Lys Ser  Leu Lys Tyr Ile Glu  Ala His Arg 
    1220                 1225                 1230             


Glu Ala  Phe Ser Glu Leu Leu  Ala Gln Val Ser Glu  Phe Ala Thr 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Asp Ala  Asn Leu Ser Lys Ile  Asn Asn Leu 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Glu Gly  Asp Ile Lys Ala Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asp Leu Met Val Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Phe Phe Glu Ala Thr  Ile Asp Arg Lys Arg  Tyr Thr Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Ser Ser  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ser Arg Lys  Arg Leu Asp Asp 
    1325                 1330                 


<210>  849
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  849

Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Glu Asn Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          


Lys Val Tyr Gly Asn Thr Glu Thr Lys Tyr Leu Lys Lys Asn Leu Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Thr Arg Arg Arg Tyr Ser Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  


Arg Leu Gln Asp Leu Phe Thr Glu Glu Met Asn Gln Val Asp Ala Asn 
                85                  90                  95      


Phe Phe His Arg Leu Gln Glu Ser Phe Leu Val Pro Asp Glu Lys Glu 
            100                 105                 110         


Phe Glu Arg His Ala Ile Phe Gly Lys Met Glu Glu Glu Val Ser Tyr 
        115                 120                 125             


Tyr Arg Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Thr Ser Glu Gln Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Val Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     


Glu Asn Ser Ser Val Ser Glu Thr Phe Arg Thr Phe Ile Gln Val Tyr 
            180                 185                 190         


Asn Gln Ile Phe Arg Glu Asn Glu Val Pro Leu Ala Val Pro Asp Asn 
        195                 200                 205             


Ile Glu Glu Leu Phe Ser Glu Lys Val Ser Arg Ala Arg Lys Val Glu 
    210                 215                 220                 


Ala Ile Leu Ser Val Tyr Ser Glu Glu Lys Ser Thr Gly Thr Leu Ala 
225                 230                 235                 240 


Gln Phe Leu Lys Leu Met Val Gly Asn Gln Gly Arg Phe Lys Lys Thr 
                245                 250                 255     


Phe Asp Leu Glu Glu Asp Gly Ile Ile Gln Ile Pro Lys Glu Glu Tyr 
            260                 265                 270         


Glu Glu Glu Leu Glu Thr Leu Leu Ala Ile Ile Gly Asp Glu Tyr Ala 
        275                 280                 285             


Glu Ile Phe Ser Ala Thr Lys Ser Val Tyr Asp Ala Val Ala Leu Ser 
    290                 295                 300                 


Gly Ile Leu Ser Val Thr Asp Gly Asp Thr Lys Ala Lys Leu Ser Ala 
305                 310                 315                 320 


Ser Met Val Glu Arg Tyr Glu Ala His Gln Lys Asp Leu Val Gln Phe 
                325                 330                 335     


Lys Gln Phe Ile Arg Lys Glu Leu Pro Glu Met Tyr Ala Pro Ile Phe 
            340                 345                 350         


Arg Asp Asn Ser Val Ser Gly Tyr Ala Gly Tyr Val Glu Asn Ser Lys 
        355                 360                 365             


Val Val Thr Gln Ala Glu Phe Tyr Lys Tyr Ile Lys Lys Ala Ile Glu 
    370                 375                 380                 


Lys Val Pro Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Thr 
385                 390                 395                 400 


Phe Leu Asp Lys Gln Arg Thr Phe Asn Asn Gly Val Ile Pro His Gln 
                405                 410                 415     


Ile His Leu Glu Glu Leu Glu Ala Ile Ile Gln Lys Gln Ala Thr Tyr 
            420                 425                 430         


Tyr Pro Phe Leu Ala Asp Asn Lys Glu Glu Met Lys Gln Leu Val Thr 
        435                 440                 445             


Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gly Asn Ser Pro 
    450                 455                 460                 


Phe Ala Trp Leu Glu Arg Ile Ser Ser Glu Pro Ile Arg Pro Gly Asn 
465                 470                 475                 480 


Leu Ala Glu Val Val Asp Ile Lys Lys Ser Ala Thr Lys Phe Ile Glu 
                485                 490                 495     


Arg Met Thr Asn Phe Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro 
            500                 505                 510         


Lys His Ser Met Ile Tyr Glu Lys Tyr Met Val Tyr Asn Glu Leu Thr 
        515                 520                 525             


Lys Val Ser Tyr Val Asp Glu Arg Gly Met Asn Gln Arg Phe Ser Gly 
    530                 535                 540                 


Glu Glu Lys Lys Gln Ile Val Glu Glu Leu Phe Lys Gln Ser Arg Lys 
545                 550                 555                 560 


Val Thr Lys Lys Leu Leu Glu Lys Phe Leu Ser Asn Glu Phe Gly Leu 
                565                 570                 575     


Val Asp Val Ala Ile Lys Gly Ile Glu Thr Ser Phe Asn Ala Gly Tyr 
            580                 585                 590         


Gly Thr Tyr His Asp Phe Leu Lys Ile Gly Ile Thr Arg Glu Gln Leu 
        595                 600                 605             


Asp Lys Glu Glu Asn Ser Glu Thr Leu Glu Glu Ile Val Lys Ile Leu 
    610                 615                 620                 


Thr Val Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Lys Lys Tyr 
625                 630                 635                 640 


Thr Tyr Leu Phe Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Lys 
            660                 665                 670         


Glu Lys Arg Thr His Lys Thr Ile Leu Asp Tyr Leu Ile Glu Asp Asp 
        675                 680                 685             


Gly Gly Lys Gln Pro Ile Asn Arg Asn Leu Met Gln Leu Ile Asn Asp 
    690                 695                 700                 


Ser Asp Leu Ser Phe Lys Ser Glu Ile Ala Glu Ala Gln Ser Asp Met 
705                 710                 715                 720 


Asn Thr Glu Asp Leu His Glu Val Val Gln Asn Leu Ala Gly Ser Pro 
                725                 730                 735     


Ala Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu 
            740                 745                 750         


Val Asp Ile Met Gly Ser Leu Pro Lys Asn Ile Val Val Glu Met Ala 
        755                 760                 765             


Arg Glu Asn Gln Thr Thr Ser Arg Gly Arg Thr Asn Ser Asn Pro Arg 
    770                 775                 780                 


Met Lys Ala Leu Glu Glu Ala Met Arg Asn Leu Arg Ser Asn Leu Leu 
785                 790                 795                 800 


Lys Glu Tyr Pro Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr 
                805                 810                 815     


Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Leu Asp Leu 
            820                 825                 830         


Ser Leu His Asn Leu Ser Ser Tyr Asp Ile Asp His Ile Val Pro Gln 
        835                 840                 845             


Ser Phe Thr Thr Asp Asn Ser Leu Asp Asn Arg Val Leu Val Ser Ser 
    850                 855                 860                 


Lys Glu Asn Arg Gly Lys Lys Asp Asp Val Pro Ser Lys Glu Val Val 
865                 870                 875                 880 


Gln Lys Asn Ile Thr Leu Trp Glu Thr Leu Lys Asn Ser Asn Leu Ile 
                885                 890                 895     


Ser Gln Lys Lys Tyr Asp Asn Leu Thr Lys Gly Leu Arg Gly Gly Leu 
            900                 905                 910         


Thr Glu Asp Asp Arg Ala His Phe Ile Lys Arg Gln Leu Val Glu Thr 
        915                 920                 925             


Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Gln Arg Phe Asn 
    930                 935                 940                 


Ser Gln Lys Asp Glu Glu Gly Lys Thr Ile Arg Ala Val Arg Val Val 
945                 950                 955                 960 


Thr Leu Lys Ser Ser Leu Thr Ser Gln Phe Arg Lys Gln Phe Ala Ile 
                965                 970                 975     


His Lys Val Arg Glu Ile Asn Asp Tyr His His Gly His Asp Ala Tyr 
            980                 985                 990         


Leu Asn Gly Val Val Ala Asn Ser  Leu Leu Arg Val Tyr  Pro Gln Leu 
        995                 1000                 1005             


Gln Pro  Glu Phe Val Tyr Gly  Asp Tyr Pro Lys Phe  Asn Ala Tyr 
    1010                 1015                 1020             


Lys Ala  Asn Lys Ala Thr Ala  Lys Lys Gln Leu Tyr  Thr Asn Ile 
    1025                 1030                 1035             


Met Lys  Phe Phe Ala Glu Asp  Ala Val Ile Ile Asp  Glu Asn Gly 
    1040                 1045                 1050             


Glu Ile  Leu Trp Asp Lys Lys  Asn Ile Ala Thr Val  Lys Lys Val 
    1055                 1060                 1065             


Met Ser  Tyr Pro Gln Met Asn  Ile Val Lys Lys Pro  Glu Ile Gln 
    1070                 1075                 1080             


Thr Gly  Ser Phe Ser Lys Glu  Thr Ile Lys Pro Lys  Gly Asp Ser 
    1085                 1090                 1095             


Asp Lys  Leu Ile Ser Arg Lys  Thr Asn Trp Ser Pro  Lys Leu Tyr 
    1100                 1105                 1110             


Gly Gly  Phe Asp Ser Pro Gln  Val Ala Tyr Ser Val  Ile Ile Thr 
    1115                 1120                 1125             


Tyr Glu  Lys Gly Lys Lys Lys  Val Arg Ala Lys Ala  Ile Val Gly 
    1130                 1135                 1140             


Ile Thr  Ile Met Glu Gln Ser  Leu Phe Lys Lys Asp  Pro Val Ser 
    1145                 1150                 1155             


Leu Leu  Glu Glu Lys Gly Tyr  Ala Asn Pro Glu Val  Leu Ile His 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Leu Glu Asn Gly  Arg Arg Arg 
    1175                 1180                 1185             


Leu Leu  Ala Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Leu Val 
    1190                 1195                 1200             


Leu Pro  Ala Ser Leu Val Thr  Leu Leu Tyr His Ala  Lys Gln Val 
    1205                 1210                 1215             


Asp Glu  Asp Ser Gly Lys Ser  Glu Glu Tyr Val Arg  Glu His Arg 
    1220                 1225                 1230             


Ala Glu  Phe Ala Glu Ile Leu  Asn Tyr Val Gln Ala  Phe Ser Glu 
    1235                 1240                 1245             


Thr Lys  Ile Leu Ala Asn Lys  Asn Leu Gln Thr Ile  Leu Lys Leu 
    1250                 1255                 1260             


Tyr Glu  Glu Asn Lys Glu Ala  Asp Ile Lys Glu Ile  Ala Glu Ser 
    1265                 1270                 1275             


Phe Val  Asn Leu Met Lys Phe  Ser Ala Tyr Gly Ala  Pro Met Asp 
    1280                 1285                 1290             


Phe Lys  Phe Phe Gly Lys Thr  Ile Pro Arg Ser Arg  Tyr Thr Ser 
    1295                 1300                 1305             


Val Gly  Glu Leu Leu Ser Ala  Thr Ile Ile Asn Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Thr Arg Arg  Lys Leu Val Asp 
    1325                 1330                 


<210>  850
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  850

Met Arg Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Gln Tyr Asn Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Val Ala Gly Ser Ala Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Glu Val Ala Ala Gly Arg Arg Met 
    50                  55                  60                  


Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ala 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Ala Glu Met Ala Glu Val Asp Ala Asn 
                85                  90                  95      


Phe Phe Tyr Arg Leu Glu Asp Ser Phe Tyr Ile Glu Ser Glu Lys Arg 
            100                 105                 110         


His Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             


His Glu Glu Tyr Lys Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 


Ser Ser Asp Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Met Leu Asp Thr 
                165                 170                 175     


Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Ile Phe Ala Ser Asp Ile Glu Glu Gly Ser Leu Thr Arg Leu 
        195                 200                 205             


Glu Glu Asn Lys Glu Val Ala Glu Ile Leu Ser Glu Lys Leu Thr Arg 
    210                 215                 220                 


Arg Glu Lys Leu Asp Lys Ile Leu Lys Leu Tyr Thr Gly Glu Lys Ser 
225                 230                 235                 240 


Thr Gly Met Phe Ala Arg Phe Ile Asn Leu Ile Ile Gly Ser Lys Gly 
                245                 250                 255     


Asp Phe Lys Lys Val Phe Asp Leu Asp Glu Lys Ala Glu Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Thr Tyr Glu Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Ile Phe Val Ala Ala Lys Ser Thr Tyr Asn 
    290                 295                 300                 


Ala Val Val Leu Ser Asn Ile Ile Thr Val Thr Asp Thr Glu Thr Lys 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Lys His Ala Lys 
                325                 330                 335     


Asp Leu Lys Arg Leu Lys Ala Phe Phe Lys Met Gln Leu Pro Glu Lys 
            340                 345                 350         


Phe Asn Glu Val Phe Asn Asp Ile Glu Lys Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Thr Gln Glu Lys Phe Tyr Lys Tyr Met Lys Lys 
    370                 375                 380                 


Met Leu Ala Asn Ile Asp Gly Ala Asp Tyr Phe Ile Asp Gln Ile Glu 
385                 390                 395                 400 


Glu Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Thr Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Arg Lys Asp Tyr Glu Lys Ile Arg Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Ile Gly Pro Leu Ala Asn Gly 
    450                 455                 460                 


Gln Ser Asp Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Ile Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Ile Arg Tyr Ile Asp Asp Gln Gly Lys Thr His His 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Gln Gln Ile Phe Asn Gly Leu Phe Lys Gln 
545                 550                 555                 560 


Gln Arg Lys Val Lys Lys Lys Asp Leu Glu Arg Phe Leu Tyr Thr Ile 
                565                 570                 575     


Asn His Ile Glu Ser Pro Thr Ile Glu Gly Val Glu Asp Ala Phe Asn 
            580                 585                 590         


Ser Ser Phe Ala Thr Tyr His Asp Leu Gln Lys Gly Gly Val Thr Gln 
        595                 600                 605             


Glu Ile Leu Asp Asn Pro Leu Asn Ala Asp Met Leu Glu Glu Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Gln Ser Phe Ser Asp Val Leu Asp Gly Thr Ile Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Thr 
            660                 665                 670         


Gly Ile Arg Asp Lys His Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala 
705                 710                 715                 720 


Asp Lys Gly Ile Gln Ser Ile Val Ala Glu Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Gly Ile Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe 
    770                 775                 780                 


Ile Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Cys Leu Lys Asn Asp Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Lys Glu Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr 
    850                 855                 860                 


Ala Asn Arg Glu Lys Gly Asp Asn Val Pro Leu Leu Glu Val Val Arg 
865                 870                 875                 880 


Lys Arg Lys Ala Phe Trp Glu Lys Leu Tyr Gln Ala Lys Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Glu Ala Asp Lys Ala Asn Phe Ile Gln Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu Tyr Gln Arg Phe Asn Cys 
    930                 935                 940                 


Lys Gln Asp Glu Asn Gly Asn Glu Val Glu Gln Val Arg Ile Val Thr 
945                 950                 955                 960 


Leu Lys Ser Thr Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Arg Phe  Phe Ala Lys Glu Asn  Gln Ile Ile Asp Lys  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Asn Arg Tyr  Leu Asp Thr Ile Lys  Lys Val Leu 
    1055                 1060                 1065             


Ser Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Asn Ala Thr  Val Asn Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Ser Arg Lys Ala  Asp Trp Asn Pro Ile  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Phe  Asp Gly Ser Asn Met  Ala Tyr Ser Ile Val  Ile Glu Tyr 
    1115                 1120                 1125             


Glu Lys  Arg Lys Lys Lys Thr  Val Ile Lys Lys Glu  Leu Ile Gln 
    1130                 1135                 1140             


Ile Asn  Ile Met Glu Arg Val  Ala Phe Glu Lys Asp  Gln Lys Ala 
    1145                 1150                 1155             


Phe Leu  Glu Glu Lys Gly Tyr  Tyr Ser Pro Lys Val  Leu Thr Lys 
    1160                 1165                 1170             


Ile Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Asn Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Gly Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Met Val 
    1190                 1195                 1200             


Leu Pro  Asn His Leu Met Thr  Leu Leu Tyr His Ala  Lys Asn Cys 
    1205                 1210                 1215             


Glu Ala  Asn Asp Gly Glu Ser  Leu Ala Tyr Ile Glu  Met His Arg 
    1220                 1225                 1230             


Glu Met  Phe Ala Glu Leu Leu  Ala Tyr Ile Ser Glu  Phe Ala Lys 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Asn Asp  Arg Leu Glu Lys Ile  Asn Met Phe 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Lys Gly  Asp Ile Lys Val Ile  Ala Lys Ser 
    1265                 1270                 1275             


Phe Asp  Lys Leu Lys Val Phe  Asn Ala Phe Gly Ala  Pro Arg Asp 
    1280                 1285                 1290             


Phe Glu  Phe Phe Glu Thr Thr  Ile Lys Arg Lys Arg  Tyr Tyr Asn 
    1295                 1300                 1305             


Ile Lys  Glu Leu Leu Asn Ala  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ala Arg Lys  Arg Leu Glu Asp 
    1325                 1330                 


<210>  851
<211>  1335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  851

Met Lys Asn Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Ile Lys Asp Asp Leu Thr Leu Val Arg Lys Lys Ile Lys 
            20                  25                  30          


Ile Ser Gly Asn Thr Asp Lys Lys Glu Val Lys Lys Asn Leu Trp Gly 
        35                  40                  45              


Ser Phe Leu Phe Glu Gln Gly Asp Thr Ala Gln Asp Thr Arg Val Lys 
    50                  55                  60                  


Arg Ile Ala Arg Arg Arg Tyr Glu Arg Arg Arg Phe Arg Ile Arg Glu 
65                  70                  75                  80  


Leu Gln Lys Ile Phe Asp Lys Ser Met Gly Glu Val Asp Ser Asn Phe 
                85                  90                  95      


Phe His Arg Leu Asp Glu Ser Phe Leu Val Glu Glu Asp Lys Glu Tyr 
            100                 105                 110         


Ser Lys Tyr Pro Ile Phe Ser Asn Glu Lys Glu Asp Lys Asn Tyr Tyr 
        115                 120                 125             


Asp Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Asp Leu Ala Asp Ser 
    130                 135                 140                 


Asn Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met 
145                 150                 155                 160 


Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Lys Met Asp 
                165                 170                 175     


Gly Ile Ser Ile Ser Glu Ser Phe Gln Glu Phe Ile Asp Ser Tyr Asn 
            180                 185                 190         


Glu Val Cys Ala Leu Glu Asp Glu Asn Leu Glu Ile Ile Tyr Asn Asp 
        195                 200                 205             


Glu Leu Leu Thr Gln Ile Glu Asn Ile Phe Lys Gln Asp Ile Ser Arg 
    210                 215                 220                 


Ser Lys Lys Leu Asp Gln Ala Ile Ala Leu Phe Gln Gly Val Lys Arg 
225                 230                 235                 240 


Gln Ser Leu Phe Gly Ile Phe Leu Thr Leu Ile Val Gly Asn Lys Ala 
                245                 250                 255     


Asn Phe Gln Lys Ile Phe Asn Leu Glu Asp Asp Ile Lys Leu Asp Leu 
            260                 265                 270         


Lys Glu Glu Asp Tyr Asp Glu Asn Leu Glu Glu Leu Leu Ser Asn Ile 
        275                 280                 285             


Asp Glu Gly Tyr Arg Asp Val Phe Leu Gln Ala Lys Asn Val Tyr Asn 
    290                 295                 300                 


Ala Ile Glu Leu Ser Lys Ile Leu Lys Thr Asp Gly Lys Glu Thr Lys 
305                 310                 315                 320 


Ala Pro Leu Ser Ala Gln Met Val Glu Leu Tyr Asn Gln His Arg Glu 
                325                 330                 335     


Asp Leu Lys Lys Tyr Lys Asp Tyr Ile Lys Ala Tyr Leu Pro Glu Lys 
            340                 345                 350         


Tyr Gly Glu Thr Phe Lys Asp Ala Thr Lys Asn Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Ser Gln Glu Asp Phe Tyr Lys Phe Val Lys Ala 
    370                 375                 380                 


Gln Leu Lys Gly Glu Glu Asn Gly Glu Tyr Phe Leu Glu Ala Ile Glu 
385                 390                 395                 400 


Asn Glu Asn Phe Leu Arg Lys Gln Arg Ser Phe Tyr Asn Gly Val Ile 
                405                 410                 415     


Pro Tyr Gln Ile His Leu Gln Glu Leu Thr Ala Val Leu Asp Gln Gln 
            420                 425                 430         


Glu Lys His Tyr Ser Phe Leu Lys Glu Asn Arg Asp Lys Ile Ile Ser 
        435                 440                 445             


Leu Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly 
    450                 455                 460                 


Glu Ser Arg Phe Ala Trp Leu Glu Arg Ser Asn Ser Glu Glu Lys Ile 
465                 470                 475                 480 


Lys Pro Trp Asn Phe Asp Lys Ile Val Asp Ile Asp Lys Ser Ala Glu 
                485                 490                 495     


Leu Phe Ile Glu Asn Leu Thr Ser Arg Asp Thr Tyr Leu Pro Asp Glu 
            500                 505                 510         


Pro Val Leu Pro Lys Arg Ser Leu Ile Tyr Gln Lys Phe Thr Ile Phe 
        515                 520                 525             


Asn Glu Leu Thr Lys Ile Ser Tyr Ile Asp Glu Arg Gly Ile Leu Gln 
    530                 535                 540                 


Asn Phe Ser Ser Arg Glu Lys Ile Ala Ile Phe Asn Asp Leu Phe Lys 
545                 550                 555                 560 


Asn Lys Ser Lys Val Thr Lys Asn Gln Leu Val Lys Tyr Ile Glu Asn 
                565                 570                 575     


Lys Glu Gln Ile Ile Ala Pro Glu Ile Lys Gly Ile Glu Asp Ser Phe 
            580                 585                 590         


Asn Ser Asn Tyr Ser Thr Tyr Ile Asp Leu Ser Lys Ile Pro Asp Met 
        595                 600                 605             


Lys Lys Leu Leu Glu Lys Asp Glu Asp Glu Ile Leu Glu Glu Ile Ile 
    610                 615                 620                 


Lys Ile Leu Thr Ile Phe Glu Asp Arg Lys Met Arg Lys Arg Gln Leu 
625                 630                 635                 640 


Met Lys Phe Lys Asp Lys Leu Ser Glu Lys Ala Ile Asn Gln Leu Ser 
                645                 650                 655     


Lys Lys His Tyr Thr Gly Trp Gly Gln Leu Ser Glu Lys Leu Ile Asn 
            660                 665                 670         


Gly Ile Arg Asp Glu Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile 
        675                 680                 685             


Glu Asp Asn Gly Cys Pro Lys Asn Met Asn Arg Asn Phe Met Gln Leu 
    690                 695                 700                 


Ile Asn Asp Asp Thr Leu Ser Phe Lys Glu Lys Ile Arg Lys Ala Gln 
705                 710                 715                 720 


Asp Ile Asn Gln Val Asn Asp Ile Lys Glu Ile Val Lys Asp Leu Pro 
                725                 730                 735     


Gly Ser Pro Ala Ile Lys Lys Gly Ile Tyr Gln Ser Ile Arg Ile Val 
            740                 745                 750         


Asp Glu Ile Ile Arg Lys Met Lys Asp Arg Pro Lys Asn Ile Val Ile 
        755                 760                 765             


Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Glu Gly Lys Asn Lys Ser 
    770                 775                 780                 


Lys Ala Arg Leu Lys Lys Ile Gln Glu Gly Leu Glu Asn Leu Asp Ser 
785                 790                 795                 800 


Val His Val Glu Lys Gln Ala Leu Asp Glu Glu Met Leu Lys Ser Pro 
                805                 810                 815     


Lys Tyr Tyr Leu Tyr Cys Leu Gln Asn Gly Lys Asp Ile Tyr Thr Gly 
            820                 825                 830         


Lys Asp Leu Asp Ile Gly Gln Leu Gln Thr Tyr Asp Ile Asp His Ile 
        835                 840                 845             


Ile Pro Arg Ser Phe Ile Thr Asp Asn Ser Phe Asp Asn Leu Val Leu 
    850                 855                 860                 


Thr Ser Ser Thr Val Asn Arg Gly Lys Leu Asp Asn Val Pro Ser Pro 
865                 870                 875                 880 


Asp Ile Val Arg Gln Gln Lys Gly Phe Trp Lys Gln Leu Leu Arg Ala 
                885                 890                 895     


Gly Leu Met Ser Gln Arg Lys Phe Asn Asn Leu Thr Lys Gly Lys Leu 
            900                 905                 910         


Thr Asp Arg Asp Arg Gln Gln Phe Ile Asn Arg Gln Leu Val Glu Thr 
        915                 920                 925             


Arg Gln Ile Thr Lys His Val Ala Asn Leu Leu Ser His His Leu Asn 
    930                 935                 940                 


Glu Lys Lys Glu Val Gly Glu Ile Asn Ile Val Leu Leu Lys Ser Ala 
945                 950                 955                 960 


Leu Thr Ser Gln Phe Arg Lys Lys Phe Asp Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Ile 
            980                 985                 990         


Ala Leu Lys Leu Leu Glu Leu Tyr  Pro Tyr Met Ala Lys  Asp Leu Ile 
        995                 1000                 1005             


Tyr Gly  Lys Tyr Ser Tyr His  Arg Lys Ile Glu Gly  Asp Lys Ala 
    1010                 1015                 1020             


Thr Gln  Ala Lys Tyr Lys Met  Ser Asn Ile Ile Glu  Arg Phe Ser 
    1025                 1030                 1035             


Gln Asp  Leu Leu Ala Asn Pro  Asp Gly Glu Ile Ala  Trp Glu Lys 
    1040                 1045                 1050             


Asp Lys  Asp Leu Asn Thr Ile  Arg Lys Val Leu Ser  Ser Lys Gln 
    1055                 1060                 1065             


Ile Asn  Ile Ile Lys Lys Ala  Glu Glu Gly Lys Gly  Arg Leu Phe 
    1070                 1075                 1080             


Lys Glu  Thr Ile Asn Ser Arg  Pro Ser Lys Lys Thr  Glu Lys Arg 
    1085                 1090                 1095             


Ile Pro  Ile Lys Asn Asn Leu  Asp Pro Asn Ile Tyr  Gly Gly Tyr 
    1100                 1105                 1110             


Ile Glu  Glu Lys Met Ala Tyr  Tyr Ile Ala Ile Asn  Tyr Leu Glu 
    1115                 1120                 1125             


Asn Gly  Lys Thr Lys Lys Ala  Ile Val Gly Ile Ser  Ile Lys Asp 
    1130                 1135                 1140             


Lys Lys  Asp Phe Glu Gly Gln  Thr Thr Glu Tyr Leu  Gly Lys Ile 
    1145                 1150                 1155             


Gly Phe  Asn Lys Ala Ser Ile  Ile Asn Ser Phe Lys  Asn Tyr Thr 
    1160                 1165                 1170             


Leu Phe  Glu Leu Glu Asn Gly  Ser Arg Arg Met Ile  Val Gly Ala 
    1175                 1180                 1185             


Ser Lys  Glu Asn Asp Ser Lys  Gly Glu Leu Gln Lys  Gly Asn Gln 
    1190                 1195                 1200             


Met Tyr  Leu Pro Gln Asn Leu  Leu Glu Phe Val Tyr  His Leu Lys 
    1205                 1210                 1215             


His Tyr  Asn Glu Asp Glu Thr  Ser His Lys Phe Ile  Val Glu His 
    1220                 1225                 1230             


Lys Ala  Tyr Phe Asp Glu Leu  Leu Asn Tyr Ile Val  Glu Phe Ala 
    1235                 1240                 1245             


Asn Lys  Tyr Leu Glu Leu Glu  Asn Ser Ile Glu Lys  Ile Lys Asp 
    1250                 1255                 1260             


Leu Tyr  His Gly Lys Gly Pro  Asp Val Glu Glu Lys  Glu Leu Val 
    1265                 1270                 1275             


Glu Ser  Phe Ile Asn Leu Leu  Ala Ile Thr Lys Cys  Gly Pro Ala 
    1280                 1285                 1290             


Ala Asp  Ile Thr Phe Leu Gly  Glu Lys Ile Ser Arg  Lys Arg Tyr 
    1295                 1300                 1305             


Arg Ser  Thr Asn Cys Leu Trp  Gly Ser Glu Val Ile  Phe Gln Ser 
    1310                 1315                 1320             


Pro Thr  Gly Leu Tyr Glu Thr  Arg Leu Arg Leu Glu  
    1325                 1330                 1335 


<210>  852
<211>  1336
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  852

Met Asp Asp Asn Tyr Gln Leu Met His Ala Lys Gly Lys Tyr Gly Tyr 
1               5                   10                  15      


Gly Thr Arg Ile Tyr Ser Glu Gly Gln Thr Ala Ala Asp Arg Arg Gly 
            20                  25                  30          


Phe Arg Thr Thr Arg Arg Arg Leu Asn Arg Arg Lys Trp Arg Leu Arg 
        35                  40                  45              


Leu Leu Gln Asp Phe Phe Glu Pro Tyr Ile Leu Pro Gln Asp Glu Ala 
    50                  55                  60                  


Phe Phe Ile Arg Gln Lys Gln Ser Asn Leu Val Pro Gln Asp Thr Lys 
65                  70                  75                  80  


Lys Gln Tyr Pro Glu Ser Val Leu Phe Asp Asp Thr Ser Asp Lys Asp 
                85                  90                  95      


Phe Tyr Thr Glu Tyr Pro Thr Ile Tyr His Leu Arg Gln Ala Leu Met 
            100                 105                 110         


Thr Glu Asn Arg Gln Phe Asp Ile Arg Glu Val Tyr Leu Ala Ile His 
        115                 120                 125             


His Ile Val Lys Tyr Arg Gly His Phe Leu Asn Asn Ala Pro Val Lys 
    130                 135                 140                 


Thr Phe Lys Ser Gly Asn Leu Asp Leu Ala Ile Lys Phe Asn Glu Leu 
145                 150                 155                 160 


Asn Glu Glu Phe Lys Ser Val Tyr Pro Asp Leu Glu Leu Gln Phe Asn 
                165                 170                 175     


Leu Asp Gln Ile Asp Glu Ile Lys Lys Ile Leu Leu Asp Ser Thr Lys 
            180                 185                 190         


Ser Arg Phe Asp Arg Gln Gly Glu Ala Val Lys Leu Ile Tyr Thr Val 
        195                 200                 205             


Ser Glu Val Lys Thr Ile Glu Lys Leu Gln Asn Lys Ile Val Lys Gln 
    210                 215                 220                 


Phe Leu Ser Gly Ile Leu Gly Leu Asn Thr Arg Phe Asp Val Leu Thr 
225                 230                 235                 240 


Asn Thr Glu Ser Asp Asp Ser Met Trp Lys Phe Ser Leu Asp Ser Glu 
                245                 250                 255     


Ser Ser Asp Asp Ala Leu Asp Glu Ile Ile Ala Gln Leu Asp Asp Pro 
            260                 265                 270         


Ala Ile His Val Val Glu Leu Val Asn Glu Ile His Ser Ser Ile Thr 
        275                 280                 285             


Leu Ala Gly Ile Val Pro Glu Gly Gln Gly Leu Ser Glu Ser Met Val 
    290                 295                 300                 


Glu Lys Tyr Glu Ala His Lys Ala His Leu Gln Leu Leu Lys Ala Val 
305                 310                 315                 320 


Glu Lys Leu Val Asp Lys Lys Ala Ala Asp Lys Leu Glu Val Ala Tyr 
                325                 330                 335     


Asp Gln Tyr Ile Asn Gly Lys Asp Ser Lys Gln Phe Ile Lys Glu Asp 
            340                 345                 350         


Phe Tyr Lys Ala Val Gln Ser Glu Ile Lys Gly Val Ala Gly Asp Glu 
        355                 360                 365             


Val Ala Glu Ile Asn Gln Leu Ile Glu Leu Asp Gln Phe Met Pro Lys 
    370                 375                 380                 


Gln Arg Thr Lys Glu Asn Gly Ala Ile Pro His Gln Leu His Gln His 
385                 390                 395                 400 


Glu Leu Asp Val Ile Ile Glu Asn Gln Ser Lys Tyr Tyr Asp Trp Leu 
                405                 410                 415     


Gly Glu Lys Val Gly Asn Glu Tyr Lys Leu Asp Lys Leu Val Ser Phe 
            420                 425                 430         


Arg Val Pro Tyr Tyr Val Gly Pro Leu Val Asp Thr Ala Ser Lys Glu 
        435                 440                 445             


Lys Lys Asp Glu Thr Arg Phe Ala Trp Met Val Arg Lys Asn Pro Glu 
    450                 455                 460                 


Ser Thr Glu Ala Ile Thr Pro Trp Asn Phe Glu Asp Ile Val Asp Lys 
465                 470                 475                 480 


Asn Ala Ser Ala Glu Asn Phe Ile Lys Arg Met Thr Thr Lys Asp Lys 
                485                 490                 495     


Tyr Leu Leu Asn Glu Asp Val Leu Pro Lys Cys Ser Leu Leu Thr Gln 
            500                 505                 510         


Asp Phe Glu Val Tyr Asn Glu Leu Asn Asn Ile Arg Val Asn Gly Glu 
        515                 520                 525             


Lys Phe Thr Ala Glu Gln Lys Gln Leu Ile His Asp Glu Leu Phe Thr 
    530                 535                 540                 


Lys His Ala Ser Val Ser Ile Lys Gln Leu Lys Asp Phe Leu Val Ser 
545                 550                 555                 560 


Glu Leu Met Tyr Val Arg Asp Asn Leu Lys Val Glu Gly Leu Ala Asp 
                565                 570                 575     


Glu Lys Lys Leu Met Ser Ser Leu Ser Thr Gln Phe Asp Tyr Gln Lys 
            580                 585                 590         


Ile Ile Pro Asp Gln Ile Asn Asp Val Lys Tyr Arg Glu Asp Ile Glu 
        595                 600                 605             


Gln Ile Ile Glu Trp Ser Thr Ile Phe Glu Asp Ala Ala Ile Phe Lys 
    610                 615                 620                 


Thr Lys Leu Lys Glu Ile Asp Trp Leu Thr Asp Glu Gln Arg Asn Lys 
625                 630                 635                 640 


Leu Ala Asn Lys Arg Tyr Arg Gly Trp Gly Ser Leu Ser Lys Arg Leu 
                645                 650                 655     


Leu Ala Cys Leu Val Asp Glu Asn Gly Gln Arg Ile Ile Asp Ile Leu 
            660                 665                 670         


Arg Asp Ser Ser Asp Asn Phe Met Met Ile Val Asn Arg Pro Glu Phe 
        675                 680                 685             


Lys Gln Ala Ile Phe Asp Glu Asn Ala Asn Leu Thr Glu Thr Asn Ser 
    690                 695                 700                 


Lys Lys Asp Met Phe Glu Met Ile Asp Asn Phe Tyr Thr Ser Pro Gln 
705                 710                 715                 720 


Asn Lys Lys Ala Ile Arg Gln Val Leu Leu Val Val Gln Asp Ile Gln 
                725                 730                 735     


Asn Ala Met Gly Gly Val Ala Pro Gln Lys Ile Phe Ile Glu Phe Ala 
            740                 745                 750         


Arg Glu Asp Glu Val Asn Gly Arg Arg Thr Asn Ser Arg Tyr Ser Gln 
        755                 760                 765             


Ile Arg Glu Met Tyr Ser Lys Val Thr Asp Asp Val Ile Asn Lys Thr 
    770                 775                 780                 


Val Arg Gly Glu Leu Glu Ala Asn Lys Gly Ser Leu Asn Lys Glu Lys 
785                 790                 795                 800 


Leu Phe Leu Tyr Phe Leu Gln Gly Gly Ile Asp Leu Tyr Ser Gly Lys 
                805                 810                 815     


Thr Ile Asn Ile Asp Asn Ile Ser Ser Tyr Asp Val Asp His Val Leu 
            820                 825                 830         


Pro Gln Ser Phe Ile Lys Asp Asp Ser Ile Asn Asn Lys Val Leu Val 
        835                 840                 845             


Asn Ala Lys Gly Asn Arg Val Lys Asn Asp Asn Val Pro Met Glu Leu 
    850                 855                 860                 


Phe Gly Ala Lys Met Tyr Pro Thr Trp Lys Arg Met Val Asp Thr Gly 
865                 870                 875                 880 


Ile Met Ser Lys Ala Lys Phe Lys Asn Leu Thr Leu Asn Pro Asp Asn 
                885                 890                 895     


Val Asp Lys Phe Lys Ala Glu Gly Phe Ile Asn Arg Gln Leu Val Glu 
            900                 905                 910         


Thr Arg Gln Ile Ile Lys Leu Val Ala Gln Ile Leu Asn Asp Lys Leu 
        915                 920                 925             


Gly Asp Asp Thr Lys Ile Val Ser Val Lys Ala Asn Leu Thr His Asp 
    930                 935                 940                 


Met Arg Val Phe Phe Asp Phe Pro Lys Asn Arg Asn Val Asn Asn Tyr 
945                 950                 955                 960 


His His Ala Phe Asp Ala Tyr Leu Ser Ala Phe Val Gly Thr Tyr Leu 
                965                 970                 975     


Tyr Lys Arg Tyr Pro Lys Leu Gln Pro Tyr Phe Val Tyr Gly Glu Phe 
            980                 985                 990         


Lys Lys Met Asp Lys Leu Lys Asn  Met Thr Ser Phe Asp  Phe Leu Arg 
        995                 1000                 1005             


Asp Leu  Lys His Thr Asp Lys  Val Val Asp Lys Asp  Thr Gly Glu 
    1010                 1015                 1020             


Ile Ile  Ala Asp Lys Leu Ala  Val Ile Thr Asn Met  Asn Lys Ile 
    1025                 1030                 1035             


Tyr Asp  Phe Lys Lys Val Leu  Val Thr His Glu Val  His Glu Asn 
    1040                 1045                 1050             


Ser Gly  Ala Met Tyr Asp Gln  Thr Leu Tyr Pro Ala  Lys Leu Asp 
    1055                 1060                 1065             


Lys Ser  Leu Gly Arg Lys Gly  Ala Lys Gln Leu Ile  Pro Lys Lys 
    1070                 1075                 1080             


Asp Asp  Met Pro Thr Ala Leu  Tyr Gly Gly Tyr Thr  Gly Ser Lys 
    1085                 1090                 1095             


Asp Ala  Phe Met Ser Ile Val  Arg Leu Lys Lys Lys  Asp Glu Val 
    1100                 1105                 1110             


Tyr Tyr  Lys Val Ile Gly Ile  Pro Val Arg Ile Ala  Asp Ser Val 
    1115                 1120                 1125             


Gln His  Gly Lys Glu Val Asp  Val Glu Ala Leu Thr  Gln Phe Ile 
    1130                 1135                 1140             


Glu Thr  Lys Phe Thr Lys Glu  Asn Leu Asn Lys Lys  Thr Gly Glu 
    1145                 1150                 1155             


Ile Thr  Arg Lys Val Glu Trp  Phe Glu Leu Val Leu  Pro Lys Val 
    1160                 1165                 1170             


Leu Phe  Gly Gln Ala Val Phe  Asp Gly Gly Gln Pro  Phe Met Leu 
    1175                 1180                 1185             


Gly Ser  Ser Thr Tyr Pro His  Asn Leu Arg Glu Leu  Val Leu Thr 
    1190                 1195                 1200             


Lys Asp  Ser Val Lys Lys Ile  Ala Gly Lys Glu Ser  Asn Lys Ser 
    1205                 1210                 1215             


Tyr Leu  Asp Ala Tyr Asp Glu  Ile Met Glu Ala Met  Asn Lys Tyr 
    1220                 1225                 1230             


Phe Pro  Leu Tyr Asp Thr Asn  Lys Phe Arg Glu Lys  Val Ile Glu 
    1235                 1240                 1245             


Gly Arg  Ser Lys Phe Ile Asp  Leu Pro Asp Lys Asp  Glu Tyr Glu 
    1250                 1255                 1260             


Asn Asn  Lys Leu Val Gln Val  Gly Lys Ser Thr Ile  Ile Asp Arg 
    1265                 1270                 1275             


Met Phe  Ile Gly Leu His Ala  Asn Ala Ala Arg Thr  Asp Leu Lys 
    1280                 1285                 1290             


Asn Ile  Gly Ile Thr Thr Pro  Phe Gly Phe Met Gln  Gln Ala Ser 
    1295                 1300                 1305             


Gly Ile  Lys Leu Thr Lys Asp  Thr Val Val Leu Phe  Gln Ser Ser 
    1310                 1315                 1320             


Thr Gly  Leu Phe Glu Arg Lys  Val Ala Leu Lys Asp  Leu 
    1325                 1330                 1335     


<210>  853
<211>  1336
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  853

Met Thr Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Asp Tyr Gln Leu Met Lys Arg Lys Met 
            20                  25                  30          


Ser Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Phe Arg Arg Thr 
    50                  55                  60                  


Lys Arg Thr Asn Arg Arg Arg Leu Ala Arg Arg Lys Tyr Arg Leu Ser 
65                  70                  75                  80  


Lys Leu Gln Asp Leu Phe Ala Glu Glu Leu Cys Lys Gln Asp Asp Cys 
                85                  90                  95      


Phe Phe Val Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Glu Lys Gln 
            100                 105                 110         


Tyr Lys Pro Ala Ser Ile Phe Pro Thr Leu Glu Glu Glu Lys Glu Tyr 
        115                 120                 125             


Tyr Gln Lys Tyr Pro Thr Ile Tyr His Leu Arg Gln Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Glu Lys Glu Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Leu Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Tyr Leu Asp Thr 
                165                 170                 175     


Glu Asn Thr Ser Ile Glu Glu Ser Phe Arg Val Phe Leu Glu Gln Tyr 
            180                 185                 190         


Gly Lys Gln Ser Asp Gln Pro Leu Ile Val His Gln Pro Val Leu Thr 
        195                 200                 205             


Ile Leu Thr Asp Lys Leu Ser Lys Thr Lys Lys Val Glu Glu Ile Leu 
    210                 215                 220                 


Lys Tyr Tyr Pro Thr Glu Lys Ile Asn Ser Phe Phe Ala Gln Cys Leu 
225                 230                 235                 240 


Lys Leu Ile Val Gly Asn Gln Ala Asn Phe Lys Arg Ile Phe Asp Leu 
                245                 250                 255     


Glu Ala Glu Val Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu Glu Asp 
            260                 265                 270         


Leu Glu Ser Leu Leu Glu Lys Ile Gly Asp Glu Tyr Leu Asp Ile Phe 
        275                 280                 285             


Leu Gln Ala Lys Lys Val His Asp Ala Ile Leu Leu Ser Glu Ile Ile 
    290                 295                 300                 


Ser Ser Thr Val Lys His Thr Gln Ala Lys Leu Ser Ser Gly Met Val 
305                 310                 315                 320 


Glu Arg Tyr Glu Arg His Lys Ala Asp Leu Ala Lys Phe Lys Gln Phe 
                325                 330                 335     


Val Lys Glu Asn Val Pro Gln Lys Ala Thr Val Phe Phe Lys Asp Thr 
            340                 345                 350         


Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Lys Gly Lys Thr Thr Gln Glu 
        355                 360                 365             


Glu Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Val Val Gly Ser 
    370                 375                 380                 


Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Thr Phe Leu Leu Lys Gln 
385                 390                 395                 400 


Arg Thr Tyr Thr Asn Gly Val Ile Pro His Gln Val His Leu Ile Glu 
                405                 410                 415     


Leu Lys Ala Ile Ile Asp Gln Gln Lys Gln His Tyr Pro Phe Leu Glu 
            420                 425                 430         


Glu Ala Gly Pro Lys Ile Ile Ala Leu Phe Lys Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Leu Ala Lys Glu Gln Glu Ala Ser Ser Phe Ala Trp 
    450                 455                 460                 


Ile Glu Arg Lys Thr Ala Glu Lys Ile His Pro Trp Asn Phe Ser Glu 
465                 470                 475                 480 


Val Val Asp Ile Glu Lys Ser Ala Met Arg Phe Ile Gln Arg Met Thr 
                485                 490                 495     


Lys Gln Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys Asn Ser 
            500                 505                 510         


Leu Leu Tyr Gln Lys Tyr Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             


Tyr Lys Asp Glu Arg Gly Val Lys Gln Tyr Phe Ser Gly Asp Glu Lys 
    530                 535                 540                 


Gln Gln Ile Phe Lys Gln Leu Phe Gln Lys Glu Arg Gly Lys Ile Thr 
545                 550                 555                 560 


Val Lys Lys Leu Gln Asn Phe Leu Tyr Thr His Tyr His Ile Glu Asn 
                565                 570                 575     


Ala His Ile Phe Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser Thr 
            580                 585                 590         


Tyr His Asp Phe Met Lys Leu Ala Lys Thr Asn Gln Lys Ser Met Gln 
        595                 600                 605             


Glu Trp Leu Glu Gln Pro Glu Met Glu Pro Ile Phe Glu Asp Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Ile Phe Glu Asp Arg Gln Met Ile Lys His Gln Leu 
625                 630                 635                 640 


Ser Lys Tyr Gln Glu Val Phe Gly Glu Lys Leu Leu Lys Glu Phe Ala 
                645                 650                 655     


Arg Lys His Tyr Thr Gly Trp Gly Arg Phe Ser Ala Lys Leu Ile His 
            660                 665                 670         


Gly Ile Arg Asp Arg Lys Thr Asn Lys Thr Ile Leu Asp Tyr Leu Ile 
        675                 680                 685             


Asn Asp Asp Asp Val Pro Ala Asn Arg Asn Arg Asn Leu Met Gln Leu 
    690                 695                 700                 


Ile Asn Asp Glu His Leu Ser Phe Lys Glu Glu Ile Ala Lys Ala Thr 
705                 710                 715                 720 


Val Phe Ser Lys His Lys Ser Leu Val Asp Val Ile Gln Asp Leu Pro 
                725                 730                 735     


Gly Ser Pro Ala Ile Lys Lys Gly Ile Trp Gln Ser Leu Lys Ile Val 
            740                 745                 750         


Glu Glu Leu Ile Ala Ile Ile Gly Tyr Lys Pro Lys Asn Ile Val Ile 
        755                 760                 765             


Glu Met Ala Arg Glu Asn Gln Lys Thr His Arg Thr Lys Pro Arg Leu 
    770                 775                 780                 


Lys Ala Leu Glu Asn Gly Leu Lys Gln Ile Gly Ser Thr Leu Leu Lys 
785                 790                 795                 800 


Glu Gln Pro Thr Asp Asn Lys Ala Leu Gln Lys Glu Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Arg Asp Ile Tyr Thr Gly Glu Pro Leu Asp 
            820                 825                 830         


Ile Glu Asn Leu His Gln Tyr Glu Val Asp His Ile Ile Pro Arg Ser 
        835                 840                 845             


Phe Ile Val Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ala Ser Lys 
    850                 855                 860                 


Gln Asn Gln Lys Lys Arg Asp Asp Val Pro Asn Lys Gln Ile Val Asn 
865                 870                 875                 880 


Glu Gln Arg Ile Phe Trp Asn Gln Leu Lys Glu Ala Lys Leu Ile Ser 
                885                 890                 895     


Pro Lys Lys Tyr Ala Tyr Leu Thr Lys Ile Glu Leu Thr Pro Glu Asp 
            900                 905                 910         


Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Asn Ile Leu His Gln Ser Phe His Gln Glu Glu Glu 
    930                 935                 940                 


Gly Thr Asp Cys Asp Gly Val Gln Ile Ile Thr Leu Lys Ala Thr Leu 
945                 950                 955                 960 


Thr Ser Gln Phe Arg Gln Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     


Asn Pro His His His Ala His Asp Ala Tyr Leu Asn Gly Phe Ile Ala 
            980                 985                 990         


Asn Val Leu Leu Lys Arg Tyr Pro  Lys Leu Ala Pro Glu  Phe Val Tyr 
        995                 1000                 1005             


Gly Lys  Tyr Val Lys Tyr Ser  Leu Ala Arg Glu Asn  Lys Ala Thr 
    1010                 1015                 1020             


Ala Lys  Lys Glu Phe Tyr Ser  Asn Ile Leu Lys Phe  Leu Glu Ser 
    1025                 1030                 1035             


Asp Glu  Pro Phe Cys Asp Glu  Asn Gly Glu Ile Tyr  Trp Glu Lys 
    1040                 1045                 1050             


Ser Tyr  His Leu Pro Arg Ile  Lys Lys Val Leu Ser  Ser His Gln 
    1055                 1060                 1065             


Val Asn  Val Val Lys Lys Val  Glu Gln Gln Lys Gly  Gly Phe Tyr 
    1070                 1075                 1080             


Lys Glu  Thr Val Asn Ser Lys  Glu Lys Pro Asp Lys  Leu Ile Glu 
    1085                 1090                 1095             


Arg Lys  Asn Asn Trp Asp Val  Thr Lys Tyr Gly Gly  Phe Gly Ser 
    1100                 1105                 1110             


Pro Val  Ile Ala Tyr Ala Ile  Ala Phe Val Tyr Ala  Lys Gly Lys 
    1115                 1120                 1125             


Thr Gln  Lys Lys Thr Arg Ala  Ile Glu Gly Ile Thr  Ile Met Glu 
    1130                 1135                 1140             


Gln Ala  Ala Phe Glu Lys Asp  Pro Thr Thr Phe Leu  Lys Asp Lys 
    1145                 1150                 1155             


Gly Phe  Pro Gln Val Thr Glu  Phe Ile Lys Leu Pro  Lys Tyr Thr 
    1160                 1165                 1170             


Leu Phe  Glu Phe Asp Asn Gly  Arg Arg Arg Phe Leu  Ala Ser His 
    1175                 1180                 1185             


Lys Glu  Ser Gln Lys Gly Asn  Pro Phe Ile Leu Ser  Asp Gln Leu 
    1190                 1195                 1200             


Val Thr  Leu Leu Tyr His Ala  Gln His Tyr Asp Lys  Ile Thr Tyr 
    1205                 1210                 1215             


Gln Glu  Ser Phe Asp Tyr Val  Asn Thr His Leu Ser  Asp Phe Ser 
    1220                 1225                 1230             


Ala Ile  Leu Thr Glu Val Leu  Ala Phe Ala Glu Lys  Tyr Thr Leu 
    1235                 1240                 1245             


Ala Asp  Lys Asn Ile Glu Arg  Ile Gln Glu Leu Tyr  Glu Glu Asn 
    1250                 1255                 1260             


Lys Tyr  Gly Glu Ile Ser Met  Ile Ala Gln Ser Phe  Leu Gln Leu 
    1265                 1270                 1275             


Leu Gln  Phe Asn Ala Ile Gly  Ala Pro Ala Asp Phe  Lys Phe Phe 
    1280                 1285                 1290             


Gly Val  Thr Ile Pro Arg Lys  Arg Tyr Thr Ser Leu  Thr Glu Ile 
    1295                 1300                 1305             


Trp Asp  Ala Thr Ile Ile Tyr  Gln Ser Val Thr Gly  Leu Tyr Glu 
    1310                 1315                 1320             


Thr Arg  Ile Arg Met Gly Asp  Leu Trp Ala Gly Glu  Gln 
    1325                 1330                 1335     


<210>  854
<211>  1336
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  854

Met Thr Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Asp Tyr Gln Leu Met Lys Arg Lys Met 
            20                  25                  30          


Ser Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Phe Arg Arg Thr 
    50                  55                  60                  


Lys Arg Thr Asn Arg Arg Arg Leu Ala Arg Arg Lys Tyr Arg Leu Ser 
65                  70                  75                  80  


Lys Leu Gln Asp Leu Phe Ala Glu Glu Leu Cys Lys Gln Asp Asp Cys 
                85                  90                  95      


Phe Phe Val Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Glu Lys Gln 
            100                 105                 110         


Tyr Lys Pro Ala Ser Ile Phe Pro Thr Leu Glu Glu Glu Lys Glu Tyr 
        115                 120                 125             


Tyr Gln Lys Tyr Pro Thr Ile Tyr His Leu Arg Gln Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Glu Lys Glu Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Leu Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Asp Thr 
                165                 170                 175     


Glu Asn Thr Ser Ile Glu Glu Ser Phe Arg Val Phe Leu Glu Gln Tyr 
            180                 185                 190         


Ser Lys Gln Ser Asp Gln Pro Leu Ile Val His Gln Pro Val Leu Thr 
        195                 200                 205             


Ile Leu Thr Asp Lys Leu Ser Lys Thr Lys Lys Val Glu Glu Ile Leu 
    210                 215                 220                 


Lys Tyr Tyr Pro Thr Glu Lys Ile Asn Ser Phe Phe Ala Gln Cys Leu 
225                 230                 235                 240 


Lys Leu Ile Val Gly Asn Gln Ala Asn Phe Lys Arg Ile Phe Asp Leu 
                245                 250                 255     


Glu Ala Glu Val Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu Glu Asp 
            260                 265                 270         


Leu Glu Ser Leu Leu Glu Lys Ile Gly Asp Glu Tyr Leu Asp Ile Phe 
        275                 280                 285             


Leu Gln Ala Lys Lys Val His Asp Ala Ile Leu Leu Ser Glu Ile Ile 
    290                 295                 300                 


Ser Ser Thr Val Lys His Thr Lys Ala Lys Leu Ser Ser Gly Met Val 
305                 310                 315                 320 


Glu Arg Tyr Glu Arg His Lys Ala Asp Leu Ala Lys Phe Lys Gln Phe 
                325                 330                 335     


Val Lys Glu Asn Val Pro Gln Lys Ala Thr Val Phe Phe Lys Asp Thr 
            340                 345                 350         


Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Lys Gly Lys Thr Thr Gln Glu 
        355                 360                 365             


Glu Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Val Val Gly Ser 
    370                 375                 380                 


Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Thr Phe Leu Leu Lys Gln 
385                 390                 395                 400 


Arg Thr Tyr Thr Asn Gly Val Ile Pro His Gln Val His Leu Ile Glu 
                405                 410                 415     


Leu Lys Ala Ile Ile Asp Gln Gln Lys Gln His Tyr Pro Phe Leu Glu 
            420                 425                 430         


Glu Ala Gly Pro Lys Ile Ile Ala Leu Phe Lys Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Leu Ala Lys Glu Gln Glu Ala Ser Ser Phe Ala Trp 
    450                 455                 460                 


Ile Glu Arg Lys Thr Ala Glu Lys Ile Asn Pro Trp Asn Phe Ser Glu 
465                 470                 475                 480 


Val Val Asp Ile Glu Lys Ser Ala Met Arg Phe Ile Gln Arg Met Thr 
                485                 490                 495     


Lys Gln Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys Asn Ser 
            500                 505                 510         


Leu Leu Tyr Gln Lys Tyr Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             


Tyr Lys Asp Glu Arg Gly Val Lys Gln Tyr Phe Ser Gly Asp Glu Lys 
    530                 535                 540                 


Gln Gln Ile Phe Lys Gln Leu Phe Gln Lys Glu Arg Gly Lys Ile Thr 
545                 550                 555                 560 


Val Lys Lys Leu Gln Asn Phe Leu Tyr Thr His Tyr His Ile Glu Asn 
                565                 570                 575     


Ala Gln Ile Phe Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser Thr 
            580                 585                 590         


Tyr His Asp Phe Met Lys Leu Ala Lys Thr Asn Gln Lys Ala Met Gln 
        595                 600                 605             


Glu Trp Leu Glu Gln Pro Glu Met Glu Pro Ile Phe Glu Asp Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Ile Phe Glu Asp Arg Gln Met Ile Lys His Gln Leu 
625                 630                 635                 640 


Ser Lys Tyr Gln Glu Val Phe Gly Glu Lys Leu Leu Lys Glu Phe Ala 
                645                 650                 655     


Arg Lys His Tyr Thr Gly Trp Gly Arg Phe Ser Ala Lys Leu Ile His 
            660                 665                 670         


Gly Ile Arg Asp Arg Lys Thr Asn Lys Thr Ile Leu Asp Tyr Leu Ile 
        675                 680                 685             


Asn Asp Asp Asp Val Pro Ala Asn Arg Asn Arg Asn Leu Met Gln Leu 
    690                 695                 700                 


Ile Asn Asp Glu His Leu Ser Phe Lys Glu Glu Ile Ala Lys Ala Thr 
705                 710                 715                 720 


Val Phe Ser Lys His Lys Ser Leu Val Asp Val Ile Gln Asp Leu Pro 
                725                 730                 735     


Gly Ser Pro Ala Ile Lys Lys Gly Ile Trp Gln Ser Leu Lys Ile Val 
            740                 745                 750         


Glu Glu Leu Ile Ala Ile Ile Gly Tyr Lys Pro Lys Asn Ile Val Ile 
        755                 760                 765             


Glu Met Ala Arg Glu Asn Gln Lys Thr His Arg Thr Ser Pro Arg Leu 
    770                 775                 780                 


Lys Ala Leu Glu Asn Gly Leu Lys Gln Ile Gly Ser Thr Leu Leu Lys 
785                 790                 795                 800 


Glu Gln Pro Thr Asp Asn Lys Ala Leu Gln Lys Glu Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Pro Leu Glu 
            820                 825                 830         


Ile Glu Asn Leu His Gln Tyr Glu Val Asp His Ile Ile Pro Arg Ser 
        835                 840                 845             


Phe Ile Val Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ala Ser Lys 
    850                 855                 860                 


Gln Asn Gln Lys Lys Arg Asp Asp Val Pro Lys Lys Gln Ile Val Asn 
865                 870                 875                 880 


Glu Gln Arg Ile Phe Trp Asn Gln Leu Lys Glu Ala Lys Leu Ile Ser 
                885                 890                 895     


Pro Lys Lys Tyr Ala Tyr Leu Thr Lys Ile Glu Leu Thr Pro Glu Asp 
            900                 905                 910         


Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Asn Ile Leu His Gln Ser Phe Asn Gln Glu Glu Glu 
    930                 935                 940                 


Gly Thr Asp Cys Asp Gly Val Gln Ile Ile Thr Leu Lys Ala Thr Leu 
945                 950                 955                 960 


Thr Ser Gln Phe Arg Gln Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     


Asn Pro His His His Ala His Asp Ala Tyr Leu Asn Gly Phe Ile Ala 
            980                 985                 990         


Asn Val Leu Leu Lys Arg Tyr Pro  Lys Leu Ala Pro Glu  Phe Val Tyr 
        995                 1000                 1005             


Gly Lys  Tyr Val Lys Tyr Ser  Leu Ala Arg Glu Asn  Lys Ala Thr 
    1010                 1015                 1020             


Ala Lys  Lys Glu Phe Tyr Ser  Asn Ile Leu Lys Phe  Leu Glu Ser 
    1025                 1030                 1035             


Asp Glu  Pro Phe Cys Asp Glu  Asn Gly Glu Ile Tyr  Trp Glu Lys 
    1040                 1045                 1050             


Ser His  His Leu Pro Arg Ile  Lys Lys Val Leu Ser  Ser His Gln 
    1055                 1060                 1065             


Val Asn  Val Val Lys Lys Val  Glu Gln Gln Lys Gly  Gly Phe Tyr 
    1070                 1075                 1080             


Lys Glu  Thr Val Asn Ser Lys  Glu Lys Pro Asp Lys  Leu Ile Glu 
    1085                 1090                 1095             


Arg Lys  Asn Asn Trp Asp Val  Thr Lys Tyr Gly Gly  Phe Gly Ser 
    1100                 1105                 1110             


Pro Val  Ile Ala Tyr Ala Ile  Ala Phe Val Tyr Ala  Lys Gly Lys 
    1115                 1120                 1125             


Thr Gln  Lys Lys Thr Arg Ala  Ile Glu Gly Ile Thr  Ile Met Glu 
    1130                 1135                 1140             


Gln Ala  Ala Phe Glu Lys Asp  Pro Thr Thr Phe Leu  Lys Glu Lys 
    1145                 1150                 1155             


Gly Phe  Pro Gln Val Thr Glu  Phe Ile Lys Leu Pro  Lys Tyr Thr 
    1160                 1165                 1170             


Leu Phe  Glu Phe Asp Asn Gly  Arg Arg Arg Phe Leu  Ala Ser His 
    1175                 1180                 1185             


Lys Glu  Ser Gln Lys Gly Asn  Pro Phe Ile Leu Ser  Asp Gln Leu 
    1190                 1195                 1200             


Val Thr  Leu Leu Tyr His Ala  Gln His Tyr Asp Lys  Ile Thr Tyr 
    1205                 1210                 1215             


Gln Glu  Ser Phe Asp Tyr Val  Asn Thr His Leu Ser  Asp Phe Ser 
    1220                 1225                 1230             


Ala Ile  Leu Thr Glu Val Leu  Ala Phe Ala Glu Lys  Tyr Thr Leu 
    1235                 1240                 1245             


Ala Asp  Lys Asn Ile Glu Arg  Ile Gln Glu Leu Tyr  Glu Glu Asn 
    1250                 1255                 1260             


Lys Tyr  Gly Glu Thr Ser Met  Ile Ala Gln Ser Phe  Leu Gln Leu 
    1265                 1270                 1275             


Leu Gln  Phe Asn Ala Ile Gly  Ala Pro Ala Asp Phe  Lys Phe Phe 
    1280                 1285                 1290             


Gly Val  Thr Ile Pro Arg Lys  Arg Tyr Thr Ser Leu  Thr Glu Ile 
    1295                 1300                 1305             


Trp Asp  Ala Thr Ile Ile Tyr  Gln Ser Val Thr Gly  Leu Tyr Glu 
    1310                 1315                 1320             


Thr Arg  Ile Arg Met Gly Asp  Leu Trp Ala Gly Glu  Gln 
    1325                 1330                 1335     


<210>  855
<211>  1337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  855

Met Lys Asn Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asn Ser Val Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys 
            20                  25                  30          


Arg Lys Met Lys Ile Ala Gly Asp Ser Glu Lys Lys Gln Ile Lys Lys 
        35                  40                  45              


Asn Phe Trp Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Ala Asp 
    50                  55                  60                  


Arg Arg Met Ala Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn 
65                  70                  75                  80  


Arg Ile Ser Tyr Leu Gln Gly Ile Phe Ala Glu Glu Met Ser Lys Thr 
                85                  90                  95      


Asp Ala Asn Phe Phe Cys Arg Leu Ser Asp Ser Phe Tyr Val Asp Asn 
            100                 105                 110         


Glu Lys Arg Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu 
        115                 120                 125             


Val Glu Tyr His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Glu Glu 
    130                 135                 140                 


Leu Val Asn Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala 
145                 150                 155                 160 


Leu Ala His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala 
                165                 170                 175     


Leu Asp Thr Gln Asn Thr Ser Val Asp Gly Ile Tyr Lys Gln Phe Ile 
            180                 185                 190         


Gln Thr Tyr Asn Gln Val Phe Ala Ser Gly Ile Glu Asp Gly Ser Leu 
        195                 200                 205             


Lys Lys Leu Glu Asp Asn Lys Asp Val Ala Lys Ile Leu Val Glu Lys 
    210                 215                 220                 


Val Thr Arg Lys Glu Lys Leu Glu Arg Ile Leu Lys Leu Tyr Pro Gly 
225                 230                 235                 240 


Glu Lys Ser Ala Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly 
                245                 250                 255     


Ser Lys Gly Asn Phe Gln Lys Pro Phe Asp Leu Ile Glu Lys Ser Asp 
            260                 265                 270         


Ile Glu Cys Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ser Leu Leu 
        275                 280                 285             


Ala Leu Ile Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn 
    290                 295                 300                 


Ala Tyr Ser Ala Val Val Leu Ser Ser Ile Ile Thr Val Ala Glu Thr 
305                 310                 315                 320 


Glu Thr Asn Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Thr 
                325                 330                 335     


His Glu Glu Asp Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu 
            340                 345                 350         


Pro Lys His Tyr Glu Glu Ile Phe Ser Asn Thr Glu Lys His Gly Tyr 
        355                 360                 365             


Ala Gly Tyr Ile Asp Gly Lys Thr Lys Gln Ala Asp Phe Tyr Lys Tyr 
    370                 375                 380                 


Met Lys Met Thr Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala 
385                 390                 395                 400 


Lys Ile Glu Lys Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn 
                405                 410                 415     


Gly Ala Ile Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu 
            420                 425                 430         


His Gln Gln Ala Lys Tyr Tyr Pro Phe Leu Lys Glu Asn Tyr Asp Lys 
        435                 440                 445             


Ile Lys Ser Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu 
    450                 455                 460                 


Ala Asn Gly Gln Ser Glu Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly 
465                 470                 475                 480 


Glu Ile Arg Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser 
                485                 490                 495     


Ala Val Asp Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro 
            500                 505                 510         


Lys Glu Asn Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Leu 
        515                 520                 525             


Val Tyr Asn Glu Leu Thr Lys Val Arg Tyr Ile Asn Asp Gln Gly Lys 
    530                 535                 540                 


Thr Ser Tyr Phe Ser Gly Gln Glu Lys Glu Gln Ile Phe Asn Asp Leu 
545                 550                 555                 560 


Phe Lys Gln Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu 
                565                 570                 575     


Arg Asn Met Ser His Val Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp 
            580                 585                 590         


Ser Phe Asn Ser Ser Tyr Ser Thr Tyr His Asp Leu Leu Lys Val Gly 
        595                 600                 605             


Ile Lys Gln Glu Ile Leu Asp Asn Pro Val Asn Thr Glu Met Leu Glu 
    610                 615                 620                 


Asn Ile Val Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys 
625                 630                 635                 640 


Glu Gln Leu Gln Gln Phe Ser Asp Val Leu Asp Gly Val Val Leu Lys 
                645                 650                 655     


Lys Leu Glu Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys 
            660                 665                 670         


Leu Leu Met Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp 
        675                 680                 685             


Tyr Leu Met Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile 
    690                 695                 700                 


Asn Asp Ser Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val 
705                 710                 715                 720 


Thr Thr Ala Asp Lys Asp Ile Gln Ser Ile Val Ala Asp Leu Ala Gly 
                725                 730                 735     


Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp 
            740                 745                 750         


Glu Leu Val Ser Val Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu 
        755                 760                 765             


Met Ala Arg Glu Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Arg 
    770                 775                 780                 


Pro Arg Tyr Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln 
785                 790                 795                 800 


Ile Leu Lys Glu His Pro Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg 
                805                 810                 815     


Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln 
            820                 825                 830         


Asp Leu Asp Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val 
        835                 840                 845             


Pro Gln Ser Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr 
    850                 855                 860                 


Ser Ser Ala Gly Asn Arg Glu Lys Gly Asp Asp Val Pro Pro Leu Glu 
865                 870                 875                 880 


Ile Val Arg Lys Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn 
                885                 890                 895     


Leu Met Ser Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly 
            900                 905                 910         


Gly Leu Thr Glu Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val 
        915                 920                 925             


Glu Thr Arg Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg 
    930                 935                 940                 


Phe Asn Tyr Glu Lys Asp Asp His Gly Asn Thr Met Lys Gln Val Arg 
945                 950                 955                 960 


Ile Val Thr Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe 
                965                 970                 975     


Gln Leu Tyr Lys Val Arg Asp Val Asn Asp Tyr His His Ala His Asp 
            980                 985                 990         


Ala Tyr Leu Asn Gly Val Val Ala  Asn Thr Leu Leu Lys  Val Tyr Pro 
        995                 1000                 1005             


Gln Leu  Glu Pro Glu Phe Val  Tyr Gly Asp Tyr His  Gln Phe Asp 
    1010                 1015                 1020             


Trp Phe  Lys Ala Asn Lys Ala  Thr Ala Lys Lys Gln  Phe Tyr Thr 
    1025                 1030                 1035             


Asn Ile  Met Leu Phe Phe Ala  Gln Lys Asp Arg Ile  Ile Asp Glu 
    1040                 1045                 1050             


Asn Gly  Glu Ile Leu Trp Asp  Lys Lys Tyr Leu Asp  Thr Val Lys 
    1055                 1060                 1065             


Lys Val  Met Ser Tyr Arg Gln  Met Asn Ile Val Lys  Lys Thr Glu 
    1070                 1075                 1080             


Ile Gln  Lys Gly Glu Phe Ser  Lys Ala Thr Ile Lys  Pro Lys Gly 
    1085                 1090                 1095             


Asn Ser  Ser Lys Leu Ile Ser  Arg Lys Thr Asn Trp  Asp Pro Met 
    1100                 1105                 1110             


Lys Tyr  Gly Gly Leu Asp Ser  Pro Asn Met Ala Tyr  Ala Val Val 
    1115                 1120                 1125             


Ile Glu  Tyr Ala Lys Gly Lys  Asn Lys Leu Val Phe  Glu Lys Lys 
    1130                 1135                 1140             


Ile Ile  Arg Val Thr Ile Met  Glu Arg Lys Ala Phe  Glu Lys Asp 
    1145                 1150                 1155             


Glu Lys  Ala Phe Leu Glu Glu  Gln Gly Tyr Arg Gln  Pro Lys Val 
    1160                 1165                 1170             


Leu Ala  Lys Leu Pro Lys Tyr  Thr Leu Tyr Glu Cys  Glu Glu Gly 
    1175                 1180                 1185             


Arg Arg  Arg Met Leu Ala Ser  Ala Asn Glu Ala Gln  Lys Gly Asn 
    1190                 1195                 1200             


Gln Gln  Val Leu Pro Asn His  Leu Val Thr Leu Leu  His His Val 
    1205                 1210                 1215             


Ala Asn  Cys Glu Val Ser Asp  Gly Lys Ser Leu Asp  Tyr Ile Glu 
    1220                 1225                 1230             


Ser Asn  Arg Glu Met Phe Ala  Glu Leu Leu Ala His  Val Ser Glu 
    1235                 1240                 1245             


Phe Ala  Lys Arg Tyr Thr Leu  Ala Glu Ala Asn Leu  Asn Lys Ile 
    1250                 1255                 1260             


Asn Gln  Leu Phe Glu Gln Asn  Lys Glu Gly Asp Ile  Lys Ala Ile 
    1265                 1270                 1275             


Ala Gln  Ser Phe Val Asp Leu  Met Ala Phe Asn Ala  Met Gly Ala 
    1280                 1285                 1290             


Pro Ala  Ser Phe Lys Phe Phe  Glu Thr Thr Ile Glu  Arg Lys Arg 
    1295                 1300                 1305             


Tyr Asn  Asn Leu Lys Glu Leu  Leu Asn Ser Thr Ile  Ile Tyr Gln 
    1310                 1315                 1320             


Ser Ile  Thr Gly Leu Tyr Glu  Ser Arg Lys Arg Leu  Asp Asp 
    1325                 1330                 1335         


<210>  856
<211>  1337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  856

Met Lys Lys Phe Glu Asn Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Ser 
1               5                   10                  15      


Ser Ile Gly Trp Ala Val Thr Asn Ser Gln Tyr Asp Ile Leu Lys Phe 
            20                  25                  30          


Asn Gly Lys Tyr Met Trp Gly Thr Arg Leu Phe Pro Glu Ala Asn Thr 
        35                  40                  45              


Ala Gln Glu Arg Arg Ile His Arg Ser Ser Arg Arg Arg Leu Lys Arg 
    50                  55                  60                  


Arg Lys Glu Arg Ile Gln Ile Leu Gln Met Leu Phe Asp Lys Glu Ile 
65                  70                  75                  80  


Ala Lys Ile Asp Ser Gly Phe Phe Gln Arg Leu Lys Asp Ser Lys Tyr 
                85                  90                  95      


Tyr Lys Glu Asp Lys Thr Glu Lys Gln Thr Asn Ser Ile Phe His Asp 
            100                 105                 110         


Lys Asp Tyr Ser Asp Lys Glu Tyr His Gln Asp Phe Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Lys Phe Leu Leu Glu Gly Asn Lys Pro Lys Asp Ile Arg 
    130                 135                 140                 


Phe Val Tyr Leu Ala Leu His His Ile Leu Thr His Arg Gly His Phe 
145                 150                 155                 160 


Leu Phe Pro Asp Met Glu Val Ser Asn Val Thr Glu Phe Ser Asn Ile 
                165                 170                 175     


Phe Ser Glu Leu Lys Gln Tyr Leu Tyr Asp Glu Met Asp Leu Asp Phe 
            180                 185                 190         


Glu Trp Lys Asn Gly Lys Leu Asn Glu Val Glu Asn Ile Leu Lys Asp 
        195                 200                 205             


Arg Asn Leu Ala Lys Ser Glu Lys Glu Lys Lys Leu Cys Gly Ile Ile 
    210                 215                 220                 


Glu Leu Glu Gly Lys Ile Asp Asn Gln Arg Lys Ala Met Ile Gly Met 
225                 230                 235                 240 


Met Cys Gly Cys Lys Lys Lys Phe Ser Asp Leu Phe Gln Asn Glu Asn 
                245                 250                 255     


Tyr Arg Glu Leu Glu Ile Ser Ser Phe Ser Phe Ala Asp Ser Ser Tyr 
            260                 265                 270         


Glu Glu Lys Arg Asp Glu Leu Glu Gly Ile Leu Ala Glu Asn Ile Leu 
        275                 280                 285             


Cys Leu Asp Tyr Leu Lys Thr Ile Tyr Asp Trp Gly Lys Leu Ser Asp 
    290                 295                 300                 


Ile Leu Gln Gly Glu Glu Ser Ile Ser Val Ala Lys Val Lys Ser Tyr 
305                 310                 315                 320 


Glu Glu His Gln Leu Asp Leu Lys Tyr Leu Lys Asn Ile Leu Lys Leu 
                325                 330                 335     


Tyr Ser His Lys Glu Lys Glu Asn Val Phe Arg Lys Lys Glu Gly Lys 
            340                 345                 350         


Tyr Pro Lys Tyr Ile Ser Gly Lys Leu Ser Gln Glu Glu Phe Asn Lys 
        355                 360                 365             


Ser Ile Lys Lys Ile Leu Glu Glu Ile Lys Ser Val Lys Gln Glu Asp 
    370                 375                 380                 


Arg Lys Val Phe Asp Thr Leu Leu Lys Arg Ala Ser Asn Asn Leu Leu 
385                 390                 395                 400 


Cys Pro Lys Gln Val Ile Lys Glu Asn Gly Val Ile Pro Tyr Gln Ile 
                405                 410                 415     


His Lys Phe Glu Leu Glu Lys Ile Leu Arg Asn Met Ala Asp Phe Phe 
            420                 425                 430         


Pro Met Leu Lys Glu Lys Lys Asp Gly Lys Thr Leu Ser Glu Lys Ile 
        435                 440                 445             


Ile Ser Val Phe Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn 
    450                 455                 460                 


Gln Asn Ser Asp Arg Ala Trp Leu Val Lys Asn Lys Asp Glu Lys Ile 
465                 470                 475                 480 


Tyr Pro Trp Asn Phe Glu Glu Ile Val Asn Leu Glu Glu Ser Ala Glu 
                485                 490                 495     


Lys Phe Ile Gln Asn Leu Thr Asn Lys Cys Thr Tyr Leu Val Leu Glu 
            500                 505                 510         


Asp Val Leu Pro Lys Ser Ser Ile Leu Tyr Ser Lys Phe Met Val Leu 
        515                 520                 525             


Asn Glu Leu Asn Asn Leu Lys Ile Asp Gly Glu Ala Ile Ser Val Asp 
    530                 535                 540                 


Leu Lys Gln Lys Ile Tyr Leu Asn Leu Phe Gln Lys Tyr Lys Lys Val 
545                 550                 555                 560 


Thr Leu Lys Lys Leu Lys Gly Tyr Leu Lys Ser Glu Asn Ile Leu Ile 
                565                 570                 575     


Asp Thr Ser Thr Gln Ile Thr Gly Ile Asp Gly Asp Phe Lys Ser Ser 
            580                 585                 590         


Leu Gly Ser Tyr Leu Asp Phe Tyr Asn Ile Leu Gly Asp Lys Val Lys 
        595                 600                 605             


Thr Asp Phe Gly Lys Lys Leu Ile Glu Asn Cys Ile Leu Trp Ile Thr 
    610                 615                 620                 


Leu Tyr Thr Gly Glu Lys Lys Leu Leu Lys Asn Lys Ile Ile Ala Asn 
625                 630                 635                 640 


Tyr Lys Gly Glu Leu Ser Glu Glu Glu Ile Lys Lys Ile Val Asn Leu 
                645                 650                 655     


Lys Tyr Lys Asp Trp Gly Arg Leu Ser Tyr Ala Phe Leu Glu Glu Ile 
            660                 665                 670         


Gln Ser Ala Ser Leu Glu Thr Gly Glu Leu Arg Asn Ile Ile Gln Met 
        675                 680                 685             


Met Trp Glu Thr Asn Asn Asn Leu Met Glu Leu Leu Ser Ser Asn Tyr 
    690                 695                 700                 


Gln Phe Leu Ser Glu Ile Glu Lys Arg Asn Ser Val Val Ala Ile Gly 
705                 710                 715                 720 


Lys Glu Phe Asn Tyr Glu Thr Ile Leu Gly Asp Ser Tyr Ala Ser Pro 
                725                 730                 735     


Ser Val Lys Arg Met Ile Trp Gln Ser Leu Ser Val Val Asp Glu Ile 
            740                 745                 750         


Lys Lys Ile Met Lys Lys Ala Pro Lys Lys Ile Phe Ile Glu Met Ala 
        755                 760                 765             


Arg Gln Glu Asp Met Lys Lys Glu Arg Lys Glu Ser Arg Lys Ser Thr 
    770                 775                 780                 


Phe Leu Thr Leu Tyr Lys Ser Ile Lys Glu Glu Gly Arg Asp Trp Ile 
785                 790                 795                 800 


Lys Glu Ile Glu Asn Trp Ser Asp Ser Glu Phe Arg Ser Lys Lys Leu 
                805                 810                 815     


Tyr Leu Tyr Tyr Thr Gln Met Gly Lys Cys Met Tyr Thr Gly Glu Lys 
            820                 825                 830         


Ile Ser Leu Asp Gln Leu Phe Asn Lys Asn Ile Tyr Asp Ile Asp His 
        835                 840                 845             


Ile Tyr Pro Arg Ser Lys Thr Lys Asp Asp Ser Ile Glu Asn Ile Val 
    850                 855                 860                 


Leu Val Lys Arg Asn Ile Asn Ala Lys Lys Thr Asp Glu Tyr Pro Leu 
865                 870                 875                 880 


Glu Arg Asn Ile Gln Gln Lys Gln His Asp Phe Trp Lys Met Leu His 
                885                 890                 895     


Ser Lys Lys Leu Ile Gly Asp Lys Lys Tyr Glu Arg Leu Thr Arg Thr 
            900                 905                 910         


Thr Glu Phe Thr Asp Glu Glu Leu Ser Asp Phe Ile Ala Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ser Thr Lys Ile Val Ala Asp Ile Leu Lys Asn 
    930                 935                 940                 


Leu Phe Pro Glu Thr Lys Ile Val Tyr Val Lys Ala Asn Leu Thr Ser 
945                 950                 955                 960 


Asp Phe Arg Lys Asn Phe Lys Ile Leu Lys Ser Arg Asp Ile Asn Asp 
                965                 970                 975     


Tyr His His Ala His Asp Ala Tyr Leu Asn Ile Val Thr Gly Asn Val 
            980                 985                 990         


Tyr Asn Ile Lys Phe Thr Asp Asn  Pro Arg Asn Phe Ile  Lys Asp Lys 
        995                 1000                 1005             


Lys Leu  Glu Gly Lys Lys Tyr  Asn Leu Lys Val Glu  Lys Ile Phe 
    1010                 1015                 1020             


Glu Tyr  Arg Lys Glu Asn Glu  Ser Phe Trp Asn Pro  Lys Thr Met 
    1025                 1030                 1035             


Val Thr  Lys Ile Glu Asn Tyr  Ile Phe Lys Lys Arg  Pro Gln Phe 
    1040                 1045                 1050             


Thr Arg  Tyr Ser Phe Glu Gln  His Gly Gly Phe Phe  Asp Gln Asn 
    1055                 1060                 1065             


Ile Val  Asp Lys Glu Ser Cys  Lys Lys Gly Thr Gly  Tyr Ile Pro 
    1070                 1075                 1080             


Ile Lys  Ser Ser Asn Ala Ile  Leu Tyr Asn Thr Ser  Lys Tyr Gly 
    1085                 1090                 1095             


Gly Tyr  Gly Ser Val Thr Gly  Thr Tyr Phe Phe Leu  Val Glu His 
    1100                 1105                 1110             


Thr Met  Lys Gly Lys Arg Ile  Arg Thr Ile Glu Met  Met Pro Leu 
    1115                 1120                 1125             


Tyr Leu  Ser Lys Lys Ile His  Ser Lys Glu Glu Leu  Glu Lys Tyr 
    1130                 1135                 1140             


Cys Lys  Glu Lys Leu Glu Leu  Gln Lys Pro Ser Val  Arg Leu Ala 
    1145                 1150                 1155             


Arg Ile  Lys Tyr Asn Ser Leu  Leu Lys Ile Asn Gly  Phe Pro Tyr 
    1160                 1165                 1170             


His Ile  Thr Gly Lys Thr Asn  Asp Ser Tyr Trp Ile  Met Ser Ala 
    1175                 1180                 1185             


Ile Gln  Leu Leu Leu Ser Lys  Asn Tyr Tyr Glu Tyr  Leu Arg Lys 
    1190                 1195                 1200             


Leu Tyr  Ile Phe Cys Lys Glu  Glu Arg Leu Glu Glu  Glu Ile Val 
    1205                 1210                 1215             


Gly Glu  Lys Asn Ile Lys Leu  Tyr Thr Cys Ile Leu  Glu Lys Leu 
    1220                 1225                 1230             


Glu Lys  Ser Ile Tyr Ser His  Lys Leu Leu Asn Ile  Asn Tyr Asn 
    1235                 1240                 1245             


Gly Lys  Ser Lys Asn Leu Lys  Asp Ile Leu Glu Ser  Glu Lys Glu 
    1250                 1255                 1260             


Asn Phe  Leu Leu Leu Ser Glu  Lys Lys Gln Ala Gln  Ile Leu Ile 
    1265                 1270                 1275             


Glu Ile  Phe Thr Leu Leu Glu  Ser Asn Asn Phe Gly  Ala Asn Leu 
    1280                 1285                 1290             


Glu Ser  Phe Gly Cys Gly Lys  Lys Cys Gly Ile Thr  Lys Ile Asn 
    1295                 1300                 1305             


Lys Asn  Ile Asp Lys Leu Glu  Glu Val Leu Leu Ile  Asn Gln Ser 
    1310                 1315                 1320             


Pro Thr  Gly Leu Phe Glu Asn  Ser Ile Asp Leu Lys  Lys Val 
    1325                 1330                 1335         


<210>  857
<211>  1337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  857

Met Lys Lys Asp Tyr Val Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Met Thr Glu Asp Tyr Gln Leu Val Lys Lys Lys Met 
            20                  25                  30          


Pro Ile Tyr Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Glu Glu Gly His Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Ile Ser Arg Arg Arg Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Ala Phe Phe Glu Glu Ala Met Thr Asp Leu Asp Glu Asn 
                85                  90                  95      


Phe Phe Ala Arg Leu Gln Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         


Trp His Arg His Pro Ile Phe Ala Lys Leu Glu Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Gln Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Val Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Ser Thr 
                165                 170                 175     


Glu Asn Ile Ser Val Lys Glu Gln Phe Gln Gln Phe Met Ile Ile Tyr 
            180                 185                 190         


Asn Gln Thr Phe Val Asn Gly Glu Ser Arg Leu Val Ser Ala Pro Leu 
        195                 200                 205             


Pro Glu Ser Val Leu Ile Glu Glu Glu Leu Thr Glu Lys Ala Ser Arg 
    210                 215                 220                 


Thr Lys Lys Ser Glu Lys Val Leu Gln Gln Phe Pro Gln Glu Lys Ala 
225                 230                 235                 240 


Asn Gly Leu Phe Gly Gln Phe Leu Lys Leu Met Val Gly Asn Lys Ala 
                245                 250                 255     


Asp Phe Lys Lys Val Phe Gly Leu Glu Glu Glu Ala Lys Ile Thr Tyr 
            260                 265                 270         


Ala Ser Glu Ser Tyr Glu Glu Asp Leu Glu Gly Ile Leu Ala Lys Val 
        275                 280                 285             


Gly Asp Glu Tyr Ser Asp Val Phe Leu Ala Ala Lys Asn Val Tyr Asp 
    290                 295                 300                 


Ala Val Glu Leu Ser Thr Ile Leu Ala Asp Ser Asp Lys Lys Ser His 
305                 310                 315                 320 


Ala Lys Leu Ser Ser Ser Met Ile Val Arg Phe Thr Glu His Gln Glu 
                325                 330                 335     


Asp Leu Lys Lys Phe Lys Arg Phe Ile Arg Glu Asn Cys Pro Asp Glu 
            340                 345                 350         


Tyr Asp Asn Leu Phe Lys Asn Glu Gln Lys Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Ala His Ala Gly Lys Val Ser Gln Leu Lys Phe Tyr Gln Tyr Val 
    370                 375                 380                 


Lys Lys Ile Ile Gln Asp Ile Ala Gly Ala Glu Tyr Phe Leu Glu Lys 
385                 390                 395                 400 


Ile Ala Gln Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
                405                 410                 415     


Val Ile Pro His Gln Ile His Leu Ala Glu Leu Gln Ala Ile Ile His 
            420                 425                 430         


Arg Gln Ala Ala Tyr Tyr Pro Phe Leu Lys Glu Asn Gln Glu Lys Ile 
        435                 440                 445             


Glu Gln Leu Val Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ser 
    450                 455                 460                 


Lys Gly Asp Ala Ser Thr Phe Ala Trp Leu Lys Arg Gln Ser Glu Glu 
465                 470                 475                 480 


Pro Ile Arg Pro Trp Asn Leu Gln Glu Thr Val Asp Leu Asp Gln Ser 
                485                 490                 495     


Ala Thr Ala Phe Ile Glu Arg Met Thr Asn Phe Asp Thr Tyr Leu Pro 
            500                 505                 510         


Ser Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Lys Phe Met 
        515                 520                 525             


Val Phe Asn Glu Leu Thr Lys Ile Ser Tyr Thr Asp Asp Arg Gly Ile 
    530                 535                 540                 


Lys Ala Asn Phe Ser Gly Lys Glu Lys Glu Lys Ile Phe Asp Tyr Leu 
545                 550                 555                 560 


Phe Lys Thr Arg Arg Lys Val Lys Lys Lys Asp Ile Ile Gln Phe Tyr 
                565                 570                 575     


Arg Asn Glu Tyr Asn Thr Glu Ile Val Thr Leu Ser Gly Leu Glu Glu 
            580                 585                 590         


Asp Gln Phe Asn Ala Ser Phe Ser Thr Tyr Gln Asp Leu Leu Lys Cys 
        595                 600                 605             


Gly Leu Thr Arg Ala Glu Leu Asp His Pro Asp Asn Ala Glu Lys Leu 
    610                 615                 620                 


Glu Asp Ile Ile Lys Ile Leu Thr Ile Phe Glu Asp Arg Gln Arg Ile 
625                 630                 635                 640 


Arg Thr Gln Leu Ser Thr Phe Lys Gly Gln Phe Ser Ala Glu Val Leu 
                645                 650                 655     


Lys Lys Leu Glu Arg Lys His Tyr Thr Gly Trp Gly Arg Leu Ser Lys 
            660                 665                 670         


Lys Leu Ile Asn Gly Ile Tyr Asp Lys Glu Ser Gly Lys Thr Ile Leu 
        675                 680                 685             


Gly Tyr Leu Ile Lys Asp Asp Gly Val Ser Lys His Tyr Asn Arg Asn 
    690                 695                 700                 


Phe Met Gln Leu Ile Asn Asp Ser Gln Leu Ser Phe Lys Asn Ala Ile 
705                 710                 715                 720 


Gln Lys Ala Gln Ser Ser Glu His Glu Glu Thr Leu Ser Glu Thr Val 
                725                 730                 735     


Asn Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Tyr Gln Ser 
            740                 745                 750         


Leu Lys Ile Val Asp Glu Leu Val Ala Ile Met Gly Tyr Ala Pro Lys 
        755                 760                 765             


Arg Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr Ser Thr Gly 
    770                 775                 780                 


Lys Arg Arg Ser Ile Gln Arg Leu Lys Ile Val Glu Lys Ala Met Ala 
785                 790                 795                 800 


Glu Ile Gly Ser Asn Leu Leu Lys Glu Gln Pro Thr Thr Asn Glu Gln 
                805                 810                 815     


Leu Arg Asp Thr Arg Leu Phe Leu Tyr Tyr Met Gln Asn Gly Lys Asp 
            820                 825                 830         


Met Tyr Thr Gly Asp Glu Leu Ser Leu His Arg Leu Ser His Tyr Asp 
        835                 840                 845             


Ile Asp His Ile Ile Pro Gln Ser Phe Met Lys Asp Asp Ser Leu Asp 
    850                 855                 860                 


Asn Leu Val Leu Val Gly Ser Thr Glu Asn Arg Gly Lys Ser Asp Asp 
865                 870                 875                 880 


Val Pro Ser Lys Glu Val Val Lys Asp Met Lys Ala Tyr Trp Glu Lys 
                885                 890                 895     


Leu Tyr Ala Ala Gly Leu Ile Ser Gln Arg Lys Phe Gln Arg Leu Thr 
            900                 905                 910         


Lys Gly Glu Gln Gly Gly Leu Thr Leu Glu Asp Lys Ala His Phe Ile 
        915                 920                 925             


Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn Val Ala Gly 
    930                 935                 940                 


Ile Leu Asp Gln Arg Tyr Asn Ala Asn Ser Lys Glu Lys Lys Val Gln 
945                 950                 955                 960 


Ile Ile Thr Leu Lys Ala Ser Leu Thr Ser Gln Phe Arg Ser Ile Phe 
                965                 970                 975     


Gly Leu Tyr Lys Val Arg Glu Val Asn Asp Tyr His His Gly Gln Asp 
            980                 985                 990         


Ala Tyr Leu Asn Cys Val Val Ala  Thr Thr Leu Leu Lys  Val Tyr Pro 
        995                 1000                 1005             


Asn Leu  Ala Pro Glu Phe Val  Tyr Gly Glu Tyr Pro  Lys Phe Gln 
    1010                 1015                 1020             


Thr Phe  Lys Glu Asn Lys Ala  Thr Ala Lys Ala Ile  Ile Tyr Thr 
    1025                 1030                 1035             


Asn Leu  Leu Arg Phe Phe Thr  Glu Asp Glu Pro Arg  Phe Thr Lys 
    1040                 1045                 1050             


Asp Gly  Glu Ile Leu Trp Ser  Asn Ser Tyr Leu Lys  Thr Ile Lys 
    1055                 1060                 1065             


Lys Glu  Leu Asn Tyr His Gln  Met Asn Ile Val Lys  Lys Val Glu 
    1070                 1075                 1080             


Val Gln  Lys Gly Gly Phe Ser  Lys Glu Ser Ile Lys  Pro Lys Gly 
    1085                 1090                 1095             


Pro Ser  Asn Lys Leu Ile Pro  Val Lys Asn Gly Leu  Asp Pro Gln 
    1100                 1105                 1110             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Ile Val Ala Tyr  Thr Val Leu 
    1115                 1120                 1125             


Phe Thr  His Glu Lys Gly Lys  Lys Pro Leu Ile Lys  Gln Glu Ile 
    1130                 1135                 1140             


Leu Gly  Ile Thr Ile Met Glu  Lys Thr Arg Phe Glu  Gln Asn Pro 
    1145                 1150                 1155             


Ile Leu  Phe Leu Glu Glu Lys  Gly Phe Leu Arg Pro  Arg Val Leu 
    1160                 1165                 1170             


Met Lys  Leu Pro Lys Tyr Thr  Leu Tyr Glu Phe Pro  Glu Gly Arg 
    1175                 1180                 1185             


Arg Arg  Leu Leu Ala Ser Ala  Lys Glu Ala Gln Lys  Gly Asn Gln 
    1190                 1195                 1200             


Met Val  Leu Pro Glu His Leu  Leu Thr Leu Leu Tyr  His Ala Lys 
    1205                 1210                 1215             


Gln Cys  Leu Leu Pro Asn Gln  Ser Glu Ser Leu Thr  Tyr Val Glu 
    1220                 1225                 1230             


Gln His  Gln Pro Glu Phe Gln  Glu Ile Leu Glu Arg  Val Val Asp 
    1235                 1240                 1245             


Phe Ala  Glu Val His Thr Leu  Ala Lys Ser Lys Val  Gln Gln Ile 
    1250                 1255                 1260             


Val Lys  Leu Phe Glu Ala Asn  Gln Thr Ala Asp Val  Lys Glu Ile 
    1265                 1270                 1275             


Ala Ala  Ser Phe Ile Gln Leu  Met Gln Phe Asn Ala  Met Gly Ala 
    1280                 1285                 1290             


Pro Ser  Thr Phe Lys Phe Phe  Gln Lys Asp Ile Glu  Arg Ala Arg 
    1295                 1300                 1305             


Tyr Thr  Ser Ile Lys Glu Ile  Phe Asp Ala Thr Ile  Ile Tyr Gln 
    1310                 1315                 1320             


Ser Thr  Thr Gly Leu Tyr Glu  Thr Arg Arg Lys Val  Val Asp 
    1325                 1330                 1335         


<210>  858
<211>  1337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  858

Met Lys Lys Asp Tyr Val Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Met Thr Glu Asp Tyr Gln Leu Val Lys Lys Lys Met 
            20                  25                  30          


Pro Ile Tyr Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Glu Glu Gly His Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Ile Ser Arg Arg Arg Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Ala Phe Phe Glu Glu Ala Met Thr Asp Leu Asp Glu Asn 
                85                  90                  95      


Phe Phe Ala Arg Leu Gln Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         


Trp His Arg His Pro Ile Phe Ala Lys Leu Glu Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Gln Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Val Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Ser Thr 
                165                 170                 175     


Glu Asn Thr Ser Val Lys Asp Gln Phe Gln Gln Phe Met Val Ile Tyr 
            180                 185                 190         


Asn Gln Thr Phe Val Asn Gly Glu Ser Arg Leu Val Ser Ala Pro Leu 
        195                 200                 205             


Pro Glu Ser Val Leu Ile Glu Glu Glu Leu Thr Glu Lys Ala Ser Arg 
    210                 215                 220                 


Thr Lys Lys Ser Glu Lys Val Leu Gln Gln Phe Pro Gln Glu Lys Ala 
225                 230                 235                 240 


Asn Gly Leu Phe Gly Gln Phe Leu Lys Leu Met Val Gly Asn Lys Ala 
                245                 250                 255     


Asp Phe Lys Lys Val Phe Gly Leu Glu Glu Glu Ala Lys Ile Thr Tyr 
            260                 265                 270         


Ala Ser Glu Ser Tyr Glu Glu Asp Leu Glu Gly Ile Leu Ala Lys Val 
        275                 280                 285             


Gly Asp Glu Tyr Ser Asp Val Phe Leu Ala Ala Lys Asn Val Tyr Asp 
    290                 295                 300                 


Ala Val Glu Leu Ser Thr Ile Leu Ala Asp Ser Asp Lys Lys Ser His 
305                 310                 315                 320 


Ala Lys Leu Ser Ser Ser Met Ile Val Arg Phe Thr Glu His Gln Glu 
                325                 330                 335     


Asp Leu Lys Lys Phe Lys Arg Phe Ile Arg Glu Asn Cys Pro Asp Glu 
            340                 345                 350         


Tyr Asp Asn Leu Phe Lys Asn Glu Gln Lys Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Ala His Ala Gly Lys Val Ser Gln Leu Lys Phe Tyr Gln Tyr Val 
    370                 375                 380                 


Lys Lys Ile Ile Gln Asp Ile Ala Gly Ala Glu Tyr Phe Leu Glu Lys 
385                 390                 395                 400 


Ile Ala Gln Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
                405                 410                 415     


Val Ile Pro His Gln Ile His Leu Ala Glu Leu Gln Ala Ile Ile His 
            420                 425                 430         


Arg Gln Ala Ala Tyr Tyr Pro Phe Leu Lys Glu Asn Gln Glu Lys Ile 
        435                 440                 445             


Glu Gln Leu Val Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ser 
    450                 455                 460                 


Lys Gly Asp Ala Ser Thr Phe Ala Trp Leu Lys Arg Gln Ser Glu Glu 
465                 470                 475                 480 


Pro Ile Arg Pro Trp Asn Leu Gln Glu Thr Val Asp Leu Asp Gln Ser 
                485                 490                 495     


Ala Thr Ala Phe Ile Glu Arg Met Thr Asn Phe Asp Thr Tyr Leu Pro 
            500                 505                 510         


Ser Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Lys Phe Met 
        515                 520                 525             


Val Phe Asn Glu Leu Thr Lys Ile Ser Tyr Thr Asp Asp Arg Gly Ile 
    530                 535                 540                 


Lys Ala Asn Phe Ser Gly Lys Glu Lys Glu Lys Ile Phe Asp Tyr Leu 
545                 550                 555                 560 


Phe Lys Thr Arg Arg Lys Val Lys Lys Lys Asp Ile Ile Gln Phe Tyr 
                565                 570                 575     


Arg Asn Glu Tyr Asn Thr Glu Ile Val Thr Leu Ser Gly Leu Glu Glu 
            580                 585                 590         


Asp Gln Phe Asn Ala Ser Phe Ser Thr Tyr Gln Asp Leu Leu Lys Cys 
        595                 600                 605             


Gly Leu Thr Arg Ala Glu Leu Asp His Pro Asp Asn Ala Glu Lys Leu 
    610                 615                 620                 


Glu Asp Ile Ile Lys Ile Leu Thr Ile Phe Glu Asp Arg Gln Arg Ile 
625                 630                 635                 640 


Arg Thr Gln Leu Ser Thr Phe Lys Gly Gln Phe Ser Ala Glu Val Leu 
                645                 650                 655     


Lys Lys Leu Glu Arg Lys His Tyr Thr Gly Trp Gly Arg Leu Ser Lys 
            660                 665                 670         


Lys Leu Ile Asn Gly Ile Tyr Asp Lys Glu Ser Gly Lys Thr Ile Leu 
        675                 680                 685             


Asp Tyr Leu Ile Lys Asp Asp Gly Val Ser Lys His Tyr Asn Arg Asn 
    690                 695                 700                 


Phe Met Gln Leu Ile Asn Asp Ser Gln Leu Ser Phe Lys Asn Ala Ile 
705                 710                 715                 720 


Gln Lys Ala Gln Ser Cys Glu His Glu Glu Thr Leu Ser Glu Thr Val 
                725                 730                 735     


Asn Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Tyr Gln Ser 
            740                 745                 750         


Leu Lys Ile Val Asp Glu Leu Val Ala Ile Met Gly Tyr Ala Pro Lys 
        755                 760                 765             


Arg Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr Ser Thr Gly 
    770                 775                 780                 


Lys Arg Arg Ser Ile Gln Arg Leu Lys Ile Val Glu Lys Ala Met Ala 
785                 790                 795                 800 


Glu Ile Gly Ser Asn Leu Leu Lys Glu Gln Pro Thr Thr Asn Glu Gln 
                805                 810                 815     


Leu Arg Asp Thr Arg Leu Phe Leu Tyr Tyr Met Gln Asn Gly Lys Asp 
            820                 825                 830         


Met Tyr Thr Gly Asp Glu Leu Ser Leu His Arg Leu Ser His Tyr Asp 
        835                 840                 845             


Ile Asp His Ile Ile Pro Gln Ser Phe Met Lys Asp Asp Ser Leu Asp 
    850                 855                 860                 


Asn Leu Val Leu Val Gly Ser Thr Glu Asn Arg Gly Lys Ser Asp Asp 
865                 870                 875                 880 


Val Pro Ser Lys Lys Val Val Lys Asp Met Lys Ala Tyr Trp Glu Lys 
                885                 890                 895     


Leu Tyr Ala Ala Gly Leu Ile Ser Gln Arg Lys Phe Gln Arg Leu Thr 
            900                 905                 910         


Lys Gly Glu Gln Gly Gly Leu Thr Leu Glu Asp Lys Ala His Phe Ile 
        915                 920                 925             


Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn Val Ala Gly 
    930                 935                 940                 


Ile Leu Asp Gln Arg Tyr Asn Ala Lys Ser Lys Glu Lys Lys Val Gln 
945                 950                 955                 960 


Ile Ile Thr Leu Lys Ala Ser Leu Thr Ser Gln Phe Arg Ser Ile Phe 
                965                 970                 975     


Gly Leu Tyr Lys Val Arg Glu Val Asn Asp Tyr His His Gly Gln Asp 
            980                 985                 990         


Ala Tyr Leu Asn Cys Val Val Ala  Thr Thr Leu Leu Lys  Val Tyr Pro 
        995                 1000                 1005             


Asn Leu  Ala Pro Glu Phe Val  Tyr Gly Glu Tyr Pro  Lys Phe Gln 
    1010                 1015                 1020             


Ala Phe  Lys Glu Asn Lys Ala  Thr Ala Lys Thr Ile  Ile Tyr Thr 
    1025                 1030                 1035             


Asn Leu  Met Arg Phe Phe Thr  Glu Asp Glu Pro Arg  Phe Thr Lys 
    1040                 1045                 1050             


Asp Ser  Glu Ile Leu Trp Ser  Asn Ser Tyr Leu Lys  Thr Ile Lys 
    1055                 1060                 1065             


Lys Glu  Leu Asn Tyr His Gln  Met Asn Ile Val Lys  Lys Val Glu 
    1070                 1075                 1080             


Val Gln  Lys Gly Gly Phe Ser  Lys Glu Ser Ile Lys  Pro Lys Gly 
    1085                 1090                 1095             


Pro Ser  Asn Lys Leu Ile Pro  Val Lys Asn Gly Leu  Asp Pro Gln 
    1100                 1105                 1110             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Val Val Ala Tyr  Thr Val Leu 
    1115                 1120                 1125             


Phe Thr  His Glu Lys Gly Lys  Lys Pro Leu Ile Lys  Gln Glu Ile 
    1130                 1135                 1140             


Leu Gly  Ile Thr Ile Met Glu  Lys Thr Arg Phe Glu  Gln Asn Pro 
    1145                 1150                 1155             


Ile Leu  Phe Leu Glu Glu Lys  Gly Phe Leu Arg Pro  Arg Val Leu 
    1160                 1165                 1170             


Met Lys  Leu Pro Lys Tyr Thr  Leu Tyr Glu Phe Pro  Glu Gly Arg 
    1175                 1180                 1185             


Arg Arg  Leu Leu Ala Ser Ala  Lys Glu Ala Gln Lys  Gly Asn Gln 
    1190                 1195                 1200             


Met Val  Leu Pro Glu His Leu  Leu Thr Leu Leu Tyr  His Ala Lys 
    1205                 1210                 1215             


Gln Cys  Leu Leu Pro Asn Gln  Ser Glu Ser Leu Ala  Tyr Val Glu 
    1220                 1225                 1230             


Gln His  Gln Pro Glu Phe Gln  Glu Ile Leu Glu Arg  Val Val Asp 
    1235                 1240                 1245             


Phe Ala  Glu Val His Thr Leu  Ala Lys Ser Lys Val  Gln Gln Ile 
    1250                 1255                 1260             


Val Lys  Leu Phe Glu Ala Asn  Gln Thr Ala Asp Val  Lys Glu Ile 
    1265                 1270                 1275             


Ala Ala  Ser Phe Ile Gln Leu  Met Gln Phe Asn Ala  Met Gly Ala 
    1280                 1285                 1290             


Pro Ser  Thr Phe Lys Phe Phe  Gln Lys Asp Ile Glu  Arg Ala Arg 
    1295                 1300                 1305             


Tyr Thr  Ser Ile Lys Glu Ile  Phe Asp Ala Thr Ile  Ile Tyr Gln 
    1310                 1315                 1320             


Ser Thr  Thr Gly Leu Tyr Glu  Thr Arg Arg Lys Val  Val Asp 
    1325                 1330                 1335         


<210>  859
<211>  1337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  859

Met Lys Lys Asp Tyr Val Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Met Thr Glu Asp Tyr Gln Leu Val Lys Lys Lys Met 
            20                  25                  30          


Pro Ile Tyr Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Glu Glu Gly His Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Ile Ser Arg Arg Arg Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Ala Phe Phe Glu Glu Ala Met Thr Ala Leu Asp Glu Asn 
                85                  90                  95      


Phe Phe Ala Arg Leu Gln Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         


Trp His Arg His Pro Ile Phe Ala Lys Leu Glu Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Gln Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Val Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Ser Thr 
                165                 170                 175     


Glu Asn Ile Ser Val Lys Glu Gln Phe Gln Gln Phe Met Ile Ile Tyr 
            180                 185                 190         


Asn Gln Thr Phe Val Asn Gly Glu Ser Arg Leu Val Ser Ala Pro Leu 
        195                 200                 205             


Pro Glu Ser Val Leu Ile Glu Glu Glu Leu Thr Glu Lys Ala Ser Arg 
    210                 215                 220                 


Thr Lys Lys Ser Glu Lys Val Leu Gln Gln Phe Pro Gln Glu Lys Ala 
225                 230                 235                 240 


Asn Gly Leu Phe Gly Gln Phe Leu Lys Leu Met Val Gly Asn Lys Ala 
                245                 250                 255     


Asp Phe Lys Lys Val Phe Gly Leu Glu Glu Glu Ala Lys Ile Thr Tyr 
            260                 265                 270         


Ala Ser Glu Ser Tyr Glu Glu Asp Leu Glu Gly Ile Leu Ala Lys Val 
        275                 280                 285             


Gly Asp Glu Tyr Ser Asp Val Phe Leu Ala Ala Lys Asn Val Tyr Asp 
    290                 295                 300                 


Ala Val Glu Leu Ser Thr Ile Leu Ala Asp Ser Asp Lys Lys Ser His 
305                 310                 315                 320 


Ala Lys Leu Ser Ser Ser Met Ile Val Arg Phe Thr Glu His Gln Glu 
                325                 330                 335     


Asp Leu Lys Asn Phe Lys Arg Phe Ile Arg Glu Asn Cys Pro Asp Glu 
            340                 345                 350         


Tyr Asp Asn Leu Phe Lys Asn Glu Gln Lys Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Thr His Ala Gly Lys Val Ser Gln Leu Lys Phe Tyr Gln Tyr Val 
    370                 375                 380                 


Lys Lys Ile Ile Gln Asp Ile Ala Gly Ala Glu Tyr Phe Leu Glu Lys 
385                 390                 395                 400 


Ile Ala Gln Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
                405                 410                 415     


Val Ile Pro His Gln Ile His Leu Ala Glu Leu Gln Ala Ile Ile His 
            420                 425                 430         


Arg Gln Ala Ala Tyr Tyr Pro Phe Leu Lys Glu Asn Gln Glu Lys Ile 
        435                 440                 445             


Glu Gln Leu Val Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ser 
    450                 455                 460                 


Lys Gly Asp Ala Asn Thr Phe Ala Trp Leu Lys Arg Gln Ser Glu Glu 
465                 470                 475                 480 


Pro Ile Arg Pro Trp Asn Leu Gln Glu Thr Val Asp Leu Asp Gln Ser 
                485                 490                 495     


Ala Thr Ala Phe Ile Glu Arg Met Thr Asn Phe Asp Thr Tyr Leu Pro 
            500                 505                 510         


Ser Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Lys Phe Met 
        515                 520                 525             


Val Phe Asn Glu Leu Thr Lys Ile Ser Tyr Thr Asp Asp Arg Gly Ile 
    530                 535                 540                 


Lys Ala Asn Phe Ser Gly Lys Glu Lys Glu Lys Ile Phe Asp Tyr Leu 
545                 550                 555                 560 


Phe Lys Thr Arg Arg Lys Val Lys Lys Lys Asp Ile Ile Gln Phe Tyr 
                565                 570                 575     


Arg Asn Glu Tyr Asn Thr Glu Ile Val Thr Leu Ser Gly Leu Glu Glu 
            580                 585                 590         


Asp Gln Phe Asn Ala Ser Phe Ser Thr Tyr Gln Asp Leu Leu Lys Cys 
        595                 600                 605             


Gly Leu Thr Arg Ala Glu Leu Asp His Pro Asp Asn Ala Glu Lys Leu 
    610                 615                 620                 


Glu Asp Ile Ile Lys Ile Leu Thr Ile Phe Glu Asp Arg Gln Arg Ile 
625                 630                 635                 640 


Arg Thr Gln Leu Ser Thr Phe Lys Gly Gln Phe Ser Ala Glu Val Leu 
                645                 650                 655     


Lys Lys Leu Glu Arg Lys His Tyr Thr Gly Trp Gly Arg Leu Ser Lys 
            660                 665                 670         


Lys Leu Ile Asn Gly Ile Tyr Asp Lys Glu Ser Gly Lys Thr Ile Leu 
        675                 680                 685             


Asp Tyr Leu Ile Lys Asp Asp Gly Val Ser Lys His Tyr Asn Arg Asn 
    690                 695                 700                 


Phe Met Gln Leu Ile Asn Asp Ser Gln Leu Ser Phe Lys Asn Ala Ile 
705                 710                 715                 720 


Gln Lys Ala Gln Ser Ser Glu His Glu Glu Thr Leu Ser Glu Thr Val 
                725                 730                 735     


Asn Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Tyr Gln Ser 
            740                 745                 750         


Leu Lys Ile Val Asp Glu Leu Val Ala Ile Met Gly Tyr Ala Pro Lys 
        755                 760                 765             


Arg Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr Ser Thr Gly 
    770                 775                 780                 


Lys Arg Arg Ser Ile Gln Arg Leu Lys Ile Val Glu Lys Ala Met Ala 
785                 790                 795                 800 


Glu Ile Gly Ser Asn Leu Leu Lys Glu Gln Pro Thr Thr Asn Glu Gln 
                805                 810                 815     


Leu Arg Asp Thr Arg Leu Phe Leu Tyr Tyr Met Gln Asn Gly Lys Asp 
            820                 825                 830         


Met Tyr Thr Gly Asp Glu Leu Ser Leu His Arg Leu Ser His Tyr Asp 
        835                 840                 845             


Ile Asp His Ile Ile Pro Gln Ser Phe Met Lys Asp Asp Ser Leu Asp 
    850                 855                 860                 


Asn Leu Val Leu Val Gly Ser Thr Glu Asn Arg Gly Lys Ser Asp Asp 
865                 870                 875                 880 


Val Pro Ser Lys Glu Val Val Lys Asp Met Lys Ala Tyr Trp Glu Lys 
                885                 890                 895     


Leu Tyr Ala Ala Gly Leu Ile Ser Gln Arg Lys Phe Gln Arg Leu Thr 
            900                 905                 910         


Lys Gly Glu Gln Gly Gly Leu Thr Leu Glu Asp Lys Ala His Phe Ile 
        915                 920                 925             


Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn Val Ala Gly 
    930                 935                 940                 


Ile Leu Asp Gln Arg Tyr Asn Ala Lys Ser Lys Glu Lys Lys Val Gln 
945                 950                 955                 960 


Ile Ile Thr Leu Lys Ala Ser Leu Thr Ser Gln Phe Arg Ser Ile Phe 
                965                 970                 975     


Gly Leu Tyr Lys Val Arg Glu Val Asn Asp Tyr His His Gly Gln Asp 
            980                 985                 990         


Ala Tyr Leu Asn Cys Val Val Ala  Thr Thr Leu Leu Lys  Val Tyr Pro 
        995                 1000                 1005             


Asn Leu  Ala Pro Glu Phe Val  Tyr Gly Glu Tyr Pro  Lys Phe Gln 
    1010                 1015                 1020             


Thr Phe  Lys Glu Asn Lys Ala  Thr Ala Lys Ala Ile  Ile Tyr Thr 
    1025                 1030                 1035             


Asn Leu  Leu Arg Phe Phe Thr  Glu Asp Glu Pro Arg  Phe Thr Lys 
    1040                 1045                 1050             


Asp Gly  Glu Ile Leu Trp Ser  Asn Ser Tyr Leu Lys  Thr Ile Lys 
    1055                 1060                 1065             


Lys Glu  Leu Asn Tyr His Gln  Met Asn Ile Val Lys  Lys Val Glu 
    1070                 1075                 1080             


Val Gln  Lys Gly Gly Phe Ser  Lys Glu Ser Ile Lys  Pro Lys Gly 
    1085                 1090                 1095             


Pro Ser  Asn Lys Leu Ile Pro  Val Lys Asn Gly Leu  Asp Pro Gln 
    1100                 1105                 1110             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Ile Val Ala Tyr  Thr Val Leu 
    1115                 1120                 1125             


Phe Thr  His Glu Lys Gly Lys  Lys Pro Leu Ile Lys  Gln Glu Ile 
    1130                 1135                 1140             


Leu Gly  Ile Thr Ile Met Glu  Lys Thr Arg Phe Glu  Gln Asn Pro 
    1145                 1150                 1155             


Ile Leu  Phe Leu Glu Glu Lys  Gly Phe Leu Arg Pro  Arg Val Leu 
    1160                 1165                 1170             


Met Lys  Leu Pro Lys Tyr Thr  Leu Tyr Gln Phe Pro  Glu Gly Arg 
    1175                 1180                 1185             


Arg Arg  Leu Leu Ala Ser Ala  Lys Glu Ala Gln Lys  Gly Asn Gln 
    1190                 1195                 1200             


Met Val  Leu Pro Glu His Leu  Leu Thr Leu Leu Tyr  His Ala Lys 
    1205                 1210                 1215             


Gln Cys  Leu Leu Pro Asn Gln  Ser Glu Ser Leu Ala  Tyr Val Glu 
    1220                 1225                 1230             


Gln His  Gln Pro Glu Phe Gln  Glu Ile Leu Glu Arg  Val Val Asp 
    1235                 1240                 1245             


Phe Ala  Glu Val His Thr Leu  Ala Lys Ser Lys Val  Gln Gln Ile 
    1250                 1255                 1260             


Val Lys  Leu Phe Glu Ala Asn  Gln Thr Ala Asp Val  Lys Glu Ile 
    1265                 1270                 1275             


Ala Ala  Ser Phe Ile Gln Leu  Met Gln Phe Asn Ala  Met Gly Ala 
    1280                 1285                 1290             


Pro Ser  Thr Phe Lys Phe Phe  Gln Lys Asp Ile Glu  Arg Ala Arg 
    1295                 1300                 1305             


Tyr Thr  Ser Ile Lys Glu Ile  Phe Asp Ala Thr Ile  Ile Tyr Gln 
    1310                 1315                 1320             


Ser Thr  Thr Gly Leu Tyr Glu  Thr Arg Arg Lys Val  Val Asp 
    1325                 1330                 1335         


<210>  860
<211>  1337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  860

Met Lys Lys Asp Tyr Val Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Met Thr Glu Asp Tyr Gln Leu Val Lys Lys Lys Met 
            20                  25                  30          


Pro Ile Tyr Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Glu Glu Gly His Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Ile Ser Arg Arg Arg Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Ala Phe Phe Glu Glu Ala Met Thr Asp Leu Asp Glu Asn 
                85                  90                  95      


Phe Phe Ala Arg Leu Gln Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         


Trp His Arg His Pro Ile Phe Ala Lys Leu Glu Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Gln Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Val Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Ser Thr 
                165                 170                 175     


Glu Asn Ile Ser Val Lys Glu Gln Phe Gln Gln Phe Met Ile Ile Tyr 
            180                 185                 190         


Asn Gln Thr Phe Val Asn Gly Glu Ser Arg Leu Val Ser Ala Pro Leu 
        195                 200                 205             


Pro Glu Ser Val Leu Ile Glu Glu Glu Leu Thr Glu Lys Ala Ser Arg 
    210                 215                 220                 


Thr Lys Lys Ser Glu Lys Val Leu Gln Gln Phe Pro Gln Glu Lys Ala 
225                 230                 235                 240 


Asn Gly Leu Phe Gly Gln Phe Leu Lys Leu Met Val Gly Asn Lys Ala 
                245                 250                 255     


Asp Phe Lys Lys Val Phe Gly Leu Glu Glu Glu Ala Lys Ile Thr Tyr 
            260                 265                 270         


Ala Ser Glu Ser Tyr Glu Glu Asp Leu Glu Gly Ile Leu Ala Lys Val 
        275                 280                 285             


Gly Asp Glu Tyr Ser Asp Val Phe Leu Ala Ala Lys Asn Val Tyr Asp 
    290                 295                 300                 


Ala Val Glu Leu Ser Thr Ile Leu Ala Asp Ser Asp Lys Lys Ser His 
305                 310                 315                 320 


Ala Lys Leu Ser Ser Ser Met Ile Val Arg Phe Thr Glu His Gln Glu 
                325                 330                 335     


Asp Leu Lys Lys Phe Lys Arg Phe Ile Arg Glu Asn Cys Pro Asp Glu 
            340                 345                 350         


Tyr Asp Asn Leu Phe Lys Asn Glu Gln Lys Asp Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Ala His Ala Gly Lys Val Ser Gln Leu Lys Phe Tyr Gln Tyr Val 
    370                 375                 380                 


Lys Lys Ile Ile Gln Asp Ile Ala Gly Ala Glu Tyr Phe Leu Glu Lys 
385                 390                 395                 400 


Ile Ala Gln Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
                405                 410                 415     


Val Ile Pro His Gln Ile His Leu Ala Glu Leu Gln Ala Ile Ile His 
            420                 425                 430         


Arg Gln Ala Ala Tyr Tyr Pro Phe Leu Lys Glu Asn Gln Glu Lys Ile 
        435                 440                 445             


Glu Gln Leu Val Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ser 
    450                 455                 460                 


Lys Gly Asp Ala Ser Thr Phe Ala Trp Leu Lys Arg Gln Ser Glu Glu 
465                 470                 475                 480 


Pro Ile Arg Pro Trp Asn Leu Gln Glu Thr Val Asp Leu Asp Gln Ser 
                485                 490                 495     


Ala Thr Ala Phe Ile Glu Arg Met Thr Asn Phe Asp Thr Tyr Leu Pro 
            500                 505                 510         


Ser Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Lys Phe Met 
        515                 520                 525             


Val Phe Asn Glu Leu Thr Lys Ile Ser Tyr Thr Asp Asp Arg Gly Ile 
    530                 535                 540                 


Lys Ala Asn Phe Ser Gly Lys Glu Lys Glu Lys Ile Phe Asp Tyr Leu 
545                 550                 555                 560 


Phe Lys Thr Arg Arg Lys Val Lys Lys Lys Asp Ile Ile Gln Phe Tyr 
                565                 570                 575     


Arg Asn Glu Tyr Asn Thr Glu Ile Val Thr Leu Ser Gly Leu Glu Glu 
            580                 585                 590         


Asp Gln Phe Asn Ala Ser Phe Ser Thr Tyr Gln Asp Leu Leu Lys Cys 
        595                 600                 605             


Gly Leu Thr Arg Ala Glu Leu Asp His Pro Asp Asn Ala Glu Lys Leu 
    610                 615                 620                 


Glu Asp Ile Ile Lys Ile Leu Thr Ile Phe Glu Asp Arg Gln Arg Ile 
625                 630                 635                 640 


Arg Thr Gln Leu Ser Thr Phe Lys Gly Gln Phe Ser Ala Glu Val Leu 
                645                 650                 655     


Lys Lys Leu Glu Arg Lys His Tyr Thr Gly Trp Gly Arg Leu Ser Lys 
            660                 665                 670         


Lys Leu Ile Asn Gly Ile Tyr Asp Lys Glu Ser Gly Lys Thr Ile Leu 
        675                 680                 685             


Gly Tyr Leu Ile Lys Asp Asp Gly Val Ser Lys His Tyr Asn Arg Asn 
    690                 695                 700                 


Phe Met Gln Leu Ile Asn Asp Ser Gln Leu Ser Phe Lys Asn Ala Ile 
705                 710                 715                 720 


Gln Lys Ala Gln Ser Ser Glu His Glu Glu Thr Leu Ser Glu Thr Val 
                725                 730                 735     


Asn Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Tyr Gln Ser 
            740                 745                 750         


Leu Lys Ile Val Asp Glu Leu Val Ala Ile Met Gly Tyr Ala Pro Lys 
        755                 760                 765             


Arg Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr Ser Thr Gly 
    770                 775                 780                 


Lys Arg Arg Ser Ile Gln Arg Leu Lys Ile Val Glu Lys Ala Met Ala 
785                 790                 795                 800 


Glu Ile Gly Ser Asn Leu Leu Lys Glu Gln Pro Thr Thr Asn Glu Gln 
                805                 810                 815     


Leu Arg Asp Thr Arg Leu Phe Leu Tyr Tyr Met Gln Asn Gly Lys Asp 
            820                 825                 830         


Met Tyr Thr Gly Asp Glu Leu Ser Leu His Arg Leu Ser His Tyr Asp 
        835                 840                 845             


Ile Asp His Ile Ile Pro Gln Ser Phe Met Lys Asp Asp Ser Leu Asp 
    850                 855                 860                 


Asn Leu Val Leu Val Gly Ser Thr Glu Asn Arg Gly Lys Ser Asp Asp 
865                 870                 875                 880 


Val Pro Ser Lys Glu Val Val Lys Asp Met Lys Ala Tyr Trp Glu Lys 
                885                 890                 895     


Leu Tyr Ala Ala Gly Leu Ile Ser Gln Arg Lys Phe Gln Arg Leu Thr 
            900                 905                 910         


Lys Gly Glu Gln Gly Gly Leu Thr Leu Glu Asp Lys Ala His Phe Ile 
        915                 920                 925             


Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn Val Ala Gly 
    930                 935                 940                 


Ile Leu Asp Gln Arg Tyr Asn Ala Asn Ser Lys Glu Lys Lys Val Gln 
945                 950                 955                 960 


Ile Ile Thr Leu Lys Ala Ser Leu Thr Ser Gln Phe Arg Ser Ile Phe 
                965                 970                 975     


Gly Leu Tyr Lys Val Arg Glu Val Asn Asp Tyr His His Gly Gln Asp 
            980                 985                 990         


Ala Tyr Leu Asn Cys Val Val Ala  Thr Thr Leu Leu Lys  Val Tyr Pro 
        995                 1000                 1005             


Asn Leu  Ala Pro Glu Phe Val  Tyr Gly Glu Tyr Pro  Lys Phe Gln 
    1010                 1015                 1020             


Thr Phe  Lys Glu Asn Lys Ala  Thr Ala Lys Ala Ile  Ile Tyr Thr 
    1025                 1030                 1035             


Asn Leu  Leu Arg Phe Phe Thr  Glu Asp Glu Pro Arg  Phe Thr Lys 
    1040                 1045                 1050             


Asp Gly  Glu Ile Leu Trp Ser  Asn Ser Tyr Leu Lys  Thr Ile Lys 
    1055                 1060                 1065             


Lys Glu  Leu Asn Tyr His Gln  Met Asn Ile Val Lys  Lys Val Glu 
    1070                 1075                 1080             


Val Gln  Lys Gly Gly Phe Ser  Lys Glu Ser Ile Lys  Pro Lys Gly 
    1085                 1090                 1095             


Pro Ser  Asn Lys Leu Ile Pro  Val Lys Asn Gly Leu  Asp Pro Gln 
    1100                 1105                 1110             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Ile Val Ala Tyr  Thr Val Leu 
    1115                 1120                 1125             


Phe Thr  His Glu Lys Gly Lys  Lys Pro Leu Ile Lys  Gln Glu Ile 
    1130                 1135                 1140             


Leu Gly  Ile Thr Ile Met Glu  Lys Thr Arg Phe Glu  Gln Asn Pro 
    1145                 1150                 1155             


Ile Leu  Phe Leu Glu Glu Lys  Gly Phe Leu Arg Pro  Arg Val Leu 
    1160                 1165                 1170             


Met Lys  Leu Pro Lys Tyr Thr  Leu Tyr Glu Phe Pro  Glu Gly Arg 
    1175                 1180                 1185             


Arg Arg  Leu Leu Ala Ser Ala  Lys Glu Ala Gln Lys  Gly Asn Gln 
    1190                 1195                 1200             


Met Val  Leu Pro Glu His Leu  Leu Thr Leu Leu Tyr  His Ala Lys 
    1205                 1210                 1215             


Gln Cys  Leu Leu Pro Asn Gln  Ser Glu Ser Leu Ala  Tyr Val Glu 
    1220                 1225                 1230             


Gln His  Gln Pro Glu Phe Gln  Glu Ile Leu Glu Arg  Val Val Asp 
    1235                 1240                 1245             


Phe Ala  Glu Val His Thr Leu  Ala Lys Ser Lys Val  Gln Gln Ile 
    1250                 1255                 1260             


Val Lys  Leu Phe Glu Ala Asn  Gln Thr Ala Asp Val  Lys Glu Ile 
    1265                 1270                 1275             


Ala Ala  Ser Phe Ile Gln Leu  Met Gln Phe Asn Ala  Met Gly Ala 
    1280                 1285                 1290             


Pro Ser  Thr Phe Lys Phe Phe  Gln Lys Asp Ile Glu  Arg Ala Arg 
    1295                 1300                 1305             


Tyr Thr  Ser Ile Lys Glu Ile  Phe Asp Ala Thr Ile  Ile Tyr Gln 
    1310                 1315                 1320             


Ser Thr  Thr Gly Leu Tyr Glu  Thr Arg Arg Lys Val  Val Asp 
    1325                 1330                 1335         


<210>  861
<211>  1338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  861

Met Gln Asp Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Leu Arg Lys Lys Gly Lys 
            20                  25                  30          


Asp Leu Trp Gly Val Arg Leu Phe Lys Glu Ala Gln Pro Ala Thr Glu 
        35                  40                  45              


Arg Arg Gly His Arg Ser Ser Arg Arg Arg Thr Ile Arg Lys Ser Leu 
    50                  55                  60                  


Arg Lys Gln Leu Leu Arg Glu Leu Phe Glu Glu Glu Ile Asn Ala Ile 
65                  70                  75                  80  


Asp Lys Asp Phe Phe Leu Arg Leu Asp Glu Ser Lys Tyr Trp Leu Glu 
                85                  90                  95      


Asp Lys Lys Ile Glu Gly Lys Tyr Ser Leu Phe Asn Asp Glu Asn Phe 
            100                 105                 110         


Thr Asp Lys Asp Tyr Tyr Glu Lys Phe Pro Thr Ile Phe His Leu Arg 
        115                 120                 125             


Lys Ala Leu Ile Glu Ser Asp Asp Lys Glu Asp Ile Arg Leu Tyr Phe 
    130                 135                 140                 


Leu Ala Ile Asn Gln Met Met Lys Arg Arg Gly His Phe Leu Ile Asp 
145                 150                 155                 160 


Gly Lys Leu Ser Asn Val Glu Asp Ile Thr Pro Phe Lys Glu Cys Ile 
                165                 170                 175     


Gln Asn Leu Ile Asn Glu Glu Leu Asp Thr Glu Ile Glu Thr Glu Phe 
            180                 185                 190         


Tyr Asp Lys Ala Ile Lys Ile Val Ser Asp Tyr Ser Leu Lys Lys Thr 
        195                 200                 205             


Glu Lys Lys Asn Leu Ile Lys Asp Leu Val Lys Glu Thr Asp Ser Ile 
    210                 215                 220                 


Glu Asp Ser Lys Lys Ile Ile Asn Ile Phe Asn Leu Leu Ile Ser Gly 
225                 230                 235                 240 


Lys Ser Lys Val Asn Gly Ile Phe Asp Asp Glu Glu Ile Leu Glu Lys 
                245                 250                 255     


Ile Lys Glu Asp Asn Lys Glu Asp Phe Tyr Leu Thr Ser Glu Lys Tyr 
            260                 265                 270         


Glu Glu Asn Leu Glu Tyr Phe Ser Gln Leu Leu Asp Tyr Arg Phe Glu 
        275                 280                 285             


Leu Ile Ser Ser Leu Lys Ser Ile Tyr Asp Tyr Ile Ile Leu Gln Glu 
    290                 295                 300                 


Val Leu Lys Gly Lys Lys Tyr Leu Ser Tyr Ala Gln Val Glu Arg Tyr 
305                 310                 315                 320 


Lys Asn His Lys Glu Asp Leu Arg Lys Leu Lys Gln Leu Ile Lys Lys 
                325                 330                 335     


Tyr Asp Lys Asn Gly Lys Ile Tyr Tyr Gln Ile Phe Arg Asp Glu Asn 
            340                 345                 350         


Phe Lys Lys Gly Tyr Val Ala Tyr Leu Gly Tyr Tyr Lys Lys Asn Lys 
        355                 360                 365             


Lys Asn Val Pro Val Lys Lys Cys Asp Tyr Asp Thr Phe Ser Lys Asn 
    370                 375                 380                 


Ile Lys Ser Thr Leu Asp Lys Ile Cys Asp Lys Glu Asp Pro Leu Tyr 
385                 390                 395                 400 


Ile Glu Leu Ile Asp Lys Ile Glu Lys Gly Gly Phe Leu Leu Lys Gln 
                405                 410                 415     


Ile Val Ser Thr Asn Ser Val Ile Pro His Gln Val His Glu Leu Glu 
            420                 425                 430         


Leu Asp Lys Ile Leu Glu Asn Leu Glu Lys Asn Tyr Pro Ser Phe Lys 
        435                 440                 445             


Glu Ile Ser Asp Gly Val Ser Lys Ile Asp Lys Ile Lys Met Ile Phe 
    450                 455                 460                 


Asn Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Asp Tyr His Lys 
465                 470                 475                 480 


Asp Lys Val Asn Ser Asn Thr Trp Ile Val Lys Asn Glu Gly Phe Glu 
                485                 490                 495     


Asn Glu Asn Ile Arg Pro Trp Asn Phe Ser Lys Ile Val Asp Glu His 
            500                 505                 510         


Ala Cys Glu Glu Glu Phe Ile Lys Arg Met Ile Asn Glu Cys Ser Tyr 
        515                 520                 525             


Ile Pro Gly Glu Lys Val Leu Pro Lys Ser Ser Leu Leu Tyr Ser Glu 
    530                 535                 540                 


Tyr Met Val Leu Asn Glu Leu Asn Asn Leu Arg Val Asn Gly Asp Lys 
545                 550                 555                 560 


Leu Asn Pro Lys Ile Lys Lys Glu Ile Val Glu Asn Ile Tyr Lys Lys 
                565                 570                 575     


Glu Arg Lys Val Thr Leu Lys Lys Ile Arg Asn Phe Leu Ile Ile Asn 
            580                 585                 590         


Asn Tyr Val Asp Ser Lys Val Glu Phe Ser Gly Leu Ser Asp Gly Ile 
        595                 600                 605             


Lys Ala Asn Met Ser Ser Tyr Ile Asp Phe Ala Glu Ile Leu Asp Asp 
    610                 615                 620                 


Asn Phe Asp Asn Asn Met Val Glu Asp Leu Ile Glu Gln Ile Thr Ile 
625                 630                 635                 640 


His Val Gly Asn Val Lys Leu Leu Thr Glu Lys Ile Ser Asn Lys Tyr 
                645                 650                 655     


Pro Asn Leu Glu Lys Lys Gln Ile Asn Lys Ile Val Asn Leu Lys Phe 
            660                 665                 670         


Lys Asp Trp Ala Arg Phe Ser Arg Lys Leu Leu Val Gly Ile Glu Gly 
        675                 680                 685             


Thr Glu Lys Glu Thr Gly Glu Ile Asn Thr Ile Ile Gly Phe Leu Arg 
    690                 695                 700                 


Glu Thr Asn Glu Asn Phe Met Glu Leu Met Ser Asn Arg Tyr Thr Phe 
705                 710                 715                 720 


Ser Glu Glu Ile Ser Glu Ile Lys Lys Lys Tyr Ile Pro Asn Lys Leu 
                725                 730                 735     


Ser Lys Gln Ile Val Asp Asp Leu Tyr Val Ser Pro Ala Val Lys Lys 
            740                 745                 750         


Met Ile Trp Gln Val Leu Asn Ile Asn Asp Glu Leu Ile Lys Val Leu 
        755                 760                 765             


Gly Lys Gln Pro Lys Lys Ile Phe Ile Glu Met Ala Arg Gly Lys Glu 
    770                 775                 780                 


Glu Lys Pro Glu Val Lys Asp Ser Arg Lys Asn Lys Leu Ile Glu Leu 
785                 790                 795                 800 


Tyr Lys Asn Leu Gly Lys Glu Gly Lys Glu Trp Leu Asn Arg Ile Glu 
                805                 810                 815     


Asp Lys Thr Glu Ala Asp Phe Arg Asn Arg Ala Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Thr Gln Met Gly Arg Cys Met Tyr Thr Gly Glu Ile Ile Asp Ile Glu 
        835                 840                 845             


Arg Leu Tyr Asp Lys Glu Leu Tyr Asp Leu Asp His Ile Ile Pro Arg 
    850                 855                 860                 


Lys Met Lys Lys Asp Asp Ser Ile His Lys Asn Leu Val Leu Val Thr 
865                 870                 875                 880 


Arg Asn Ser Asn Gln Tyr Glu Lys Lys Asp Asn Tyr Pro Val Pro Glu 
                885                 890                 895     


Val Ile Lys Ser Arg Glu Asn Val Arg His Leu Trp Tyr Ala Leu Lys 
            900                 905                 910         


Lys Lys Gly Leu Ile Thr Glu Glu Lys Tyr Asn Arg Leu Thr Arg Ile 
        915                 920                 925             


Asn Pro Leu Ser Asp Lys Glu Leu Ser Glu Phe Ile Glu Arg Gln Leu 
    930                 935                 940                 


Val Glu Thr Arg Gln Thr Thr Lys Val Val Lys Asp Leu Phe Glu Asn 
945                 950                 955                 960 


Tyr Tyr Thr Asp Ser Lys Ile Val Thr Val Lys Ala Gly Leu Val Ser 
                965                 970                 975     


Glu Leu Arg Arg Asp Phe Glu Val Leu Lys Cys Arg Glu Ile Asn Asp 
            980                 985                 990         


Leu Asn His Ala His Asp Ala Phe  Leu Asn Ile Ile Val  Gly Asp Val 
        995                 1000                 1005             


Trp Ser  Lys Lys Phe Thr Ser  Asn Pro Tyr Asn Phe  Val Lys Lys 
    1010                 1015                 1020             


Asn Arg  Glu Lys Tyr Thr Leu  Asn Asn Ile Phe Thr  Lys Asn Gln 
    1025                 1030                 1035             


Lys Asp  Leu Arg Gly Asn Glu  Ile Trp Asn Cys Ala  Glu Gly Lys 
    1040                 1045                 1050             


Lys Arg  Ile Ile Lys Thr Leu  Asn Lys Pro Ser Val  Leu Phe Ser 
    1055                 1060                 1065             


Val Glu  Asn Phe Glu Gln Thr  Gly Gln Leu Phe Asp  Gln Asn Ile 
    1070                 1075                 1080             


Ile Gly  Lys Gln Asp Tyr Lys  Glu Lys Thr Ser Tyr  Val Pro Ile 
    1085                 1090                 1095             


Lys Gly  Asp Glu Arg Leu Gln  Asp Ile Ser Lys Tyr  Gly Gly Tyr 
    1100                 1105                 1110             


Arg Gly  Ile Ser Gly Ala Tyr  Phe Phe Ile Val Glu  His Thr Glu 
    1115                 1120                 1125             


Lys Asn  Lys Arg Val Lys Thr  Ile Glu Ser Leu Pro  Ile Tyr Met 
    1130                 1135                 1140             


Lys Ser  Tyr Val Glu Glu Asn  Gly Leu Leu Asp Phe  Cys Thr Asn 
    1145                 1150                 1155             


Glu Met  Lys Leu Val Asp Pro  Lys Ile Ile Val Lys  Lys Leu Asn 
    1160                 1165                 1170             


Tyr Lys  Ser Glu Ile Ile Val  Asp Gly Phe Lys Tyr  Leu Ile Ser 
    1175                 1180                 1185             


Gly Lys  Thr Glu Asn Arg Leu  Ser Leu Glu Thr Asn  Glu Tyr Leu 
    1190                 1195                 1200             


Tyr Trp  Asn His Thr Gln Thr  Thr Asp Ile Lys Lys  Ile Leu Asn 
    1205                 1210                 1215             


Ile Asn  Glu Lys Ile Glu Leu  Glu Lys Tyr Asn Gly  Asp Ile Glu 
    1220                 1225                 1230             


Ser Glu  Arg Glu Asn Ile Ile  Lys Tyr Ile Gln Ile  Ile Leu Glu 
    1235                 1240                 1245             


Lys Phe  Lys Leu Lys Pro Phe  Ile Lys Arg Arg Asn  Leu Pro Asn 
    1250                 1255                 1260             


Ile Glu  Ile Asn Phe Ala Asp  Tyr Glu Thr Glu Lys  Leu Leu Gln 
    1265                 1270                 1275             


Leu Leu  Val Asn Leu Ile Pro  Leu Thr Thr Lys Tyr  Val Ser Pro 
    1280                 1285                 1290             


Val Asp  Leu Thr Val Ile Ser  Gly Ser Lys Tyr Ala  Gly Lys Val 
    1295                 1300                 1305             


Leu Ile  Pro Lys Lys Ile Ser  Asn Leu Asn Glu Val  Ile Phe Ile 
    1310                 1315                 1320             


Ser Lys  Ser Ile Thr Gly Ile  Tyr Glu Ser Arg Arg  Arg Ile Ile 
    1325                 1330                 1335             


<210>  862
<211>  1338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  862

Met Lys Gln Glu Tyr Phe Leu Gly Leu Asp Met Gly Thr Gly Ser Leu 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Ser Thr Tyr Gln Val Met Arg Lys His Gly 
            20                  25                  30          


Lys Ala Leu Trp Gly Thr Arg Leu Phe Glu Ser Ala Ser Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Met Phe Arg Thr Ala Arg Arg Arg Leu Asp Arg Arg Asn 
    50                  55                  60                  


Trp Arg Ile Gln Val Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys 
65                  70                  75                  80  


Val Asp Pro Gly Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro 
                85                  90                  95      


Glu Asp Lys Arg Asp Ala Glu Gly Asn Cys Pro Glu Leu Pro Tyr Ala 
            100                 105                 110         


Leu Phe Val Asp Asp Asn Tyr Thr Asp Lys Asn Tyr His Lys Asp Tyr 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu Thr Thr Glu Ile 
    130                 135                 140                 


Pro Asp Ile Arg Leu Val Tyr Leu Val Leu His His Met Met Lys His 
145                 150                 155                 160 


Arg Gly His Phe Leu Leu Ser Gly Asp Ile Ser Gln Ile Lys Glu Phe 
                165                 170                 175     


Lys Ser Thr Phe Glu Gln Leu Ile Gln Asn Ile Gln Asp Glu Glu Leu 
            180                 185                 190         


Glu Trp His Ile Ser Leu Asp Asp Ala Ala Ile Gln Phe Val Glu His 
        195                 200                 205             


Val Leu Lys Asp Arg Asn Leu Thr Arg Ser Thr Lys Lys Ser Arg Leu 
    210                 215                 220                 


Ile Lys Gln Leu Asn Ala Lys Ser Ala Cys Glu Lys Ala Ile Leu Asn 
225                 230                 235                 240 


Leu Leu Ser Gly Gly Thr Val Lys Leu Ser Asp Ile Phe Asn Asn Lys 
                245                 250                 255     


Glu Leu Asp Glu Ser Glu Arg Pro Lys Val Ser Phe Ala Asp Ser Gly 
            260                 265                 270         


Tyr Asp Asp Tyr Ile Gly Ile Val Glu Ala Glu Leu Ala Glu Gln Tyr 
        275                 280                 285             


Tyr Ile Ile Ala Ser Ala Lys Ala Val Tyr Asp Trp Ser Val Leu Val 
    290                 295                 300                 


Glu Ile Leu Gly Asn Ser Val Ser Ile Ser Glu Ala Lys Ile Lys Val 
305                 310                 315                 320 


Tyr Gln Lys His Gln Ala Asp Leu Lys Thr Leu Lys Lys Ile Val Arg 
                325                 330                 335     


Gln Tyr Met Thr Lys Glu Asp Tyr Lys Arg Val Phe Val Asp Thr Glu 
            340                 345                 350         


Glu Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Gly Met Thr Lys Lys Asn 
        355                 360                 365             


Gly Lys Lys Val Asp Leu Lys Ser Lys Gln Cys Thr Gln Ala Asp Phe 
    370                 375                 380                 


Tyr Asp Phe Leu Lys Lys Asn Val Ile Lys Val Ile Asp His Lys Glu 
385                 390                 395                 400 


Ile Thr Gln Glu Ile Glu Ser Glu Ile Glu Lys Glu Asn Phe Leu Pro 
                405                 410                 415     


Lys Gln Val Thr Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Asp 
            420                 425                 430         


Tyr Glu Leu Lys Lys Ile Leu Asp Asn Leu Gly Thr Arg Met Pro Phe 
        435                 440                 445             


Ile Lys Glu Asn Ala Glu Lys Ile Gln Gln Leu Phe Glu Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Asn Arg Val Asp Asp Gly Lys Asp Gly 
465                 470                 475                 480 


Lys Phe Thr Trp Ser Val Arg Lys Ser Asp Ala Arg Ile Tyr Pro Trp 
                485                 490                 495     


Asn Phe Thr Glu Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile 
            500                 505                 510         


Arg Arg Met Thr Asn Lys Cys Thr Tyr Leu Val Gly Glu Asp Val Leu 
        515                 520                 525             


Pro Lys Asp Ser Leu Val Tyr Ser Lys Phe Met Val Leu Asn Glu Leu 
    530                 535                 540                 


Asn Asn Leu Arg Leu Asn Gly Glu Lys Ile Ser Val Glu Leu Lys Gln 
545                 550                 555                 560 


Arg Ile Tyr Glu Glu Leu Phe Cys Lys Tyr Arg Lys Val Thr Arg Lys 
                565                 570                 575     


Lys Leu Glu Arg Tyr Leu Val Ile Glu Gly Ile Ala Lys Lys Gly Val 
            580                 585                 590         


Glu Ile Thr Gly Ile Asp Gly Asp Phe Lys Ala Ser Leu Thr Ala Tyr 
        595                 600                 605             


His Asp Phe Lys Glu Arg Leu Thr Asp Val Gln Leu Ser Gln Arg Ala 
    610                 615                 620                 


Lys Glu Ala Ile Val Leu Asn Val Val Leu Phe Gly Asp Asp Lys Lys 
625                 630                 635                 640 


Leu Leu Lys Gln Arg Leu Ser Lys Met Tyr Pro Asn Leu Thr Thr Gly 
                645                 650                 655     


Gln Leu Lys Gly Ile Cys Ser Leu Ser Tyr Gln Gly Trp Gly Arg Leu 
            660                 665                 670         


Ser Lys Thr Phe Leu Glu Glu Ile Thr Val Pro Ala Pro Gly Thr Gly 
        675                 680                 685             


Glu Val Trp Asn Ile Met Thr Ala Leu Trp Gln Thr Asn Asp Asn Leu 
    690                 695                 700                 


Met Gln Leu Leu Ser Arg Asn Tyr Gly Phe Thr Asn Glu Val Glu Glu 
705                 710                 715                 720 


Phe Asn Thr Leu Lys Lys Glu Thr Asp Leu Ser Tyr Lys Thr Val Asp 
                725                 730                 735     


Glu Leu Tyr Val Ser Pro Ala Val Lys Arg Gln Ile Trp Gln Thr Leu 
            740                 745                 750         


Lys Val Val Lys Glu Ile Gln Lys Val Met Gly Asn Ala Pro Lys Arg 
        755                 760                 765             


Val Phe Val Glu Met Ala Arg Glu Lys Gln Glu Gly Lys Arg Ser Asp 
    770                 775                 780                 


Ser Arg Lys Lys Gln Leu Val Glu Leu Tyr Arg Ala Cys Lys Asn Glu 
785                 790                 795                 800 


Glu Arg Asp Trp Ile Thr Glu Leu Asn Ala Gln Ser Asp Gln Gln Leu 
                805                 810                 815     


Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Ile Gln Lys Gly Arg Cys Met 
            820                 825                 830         


Tyr Ser Gly Glu Thr Ile Gln Leu Asp Glu Leu Trp Asp Asn Thr Lys 
        835                 840                 845             


Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met Asp Asp Ser 
    850                 855                 860                 


Leu Asn Asn Arg Val Leu Val Lys Lys Asn Tyr Asn Ala Ile Lys Ser 
865                 870                 875                 880 


Asp Thr Tyr Pro Leu Ser Leu Asp Ile Gln Lys Lys Met Met Ser Phe 
                885                 890                 895     


Trp Lys Met Leu Gln Gln Gln Gly Phe Ile Thr Lys Glu Lys Tyr Val 
            900                 905                 910         


Arg Leu Val Arg Ser Asp Glu Leu Ser Ala Asp Glu Leu Ala Gly Phe 
        915                 920                 925             


Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ser Thr Lys Ala Val Ala 
    930                 935                 940                 


Thr Ile Leu Lys Glu Ala Leu Pro Asp Thr Glu Ile Val Tyr Val Lys 
945                 950                 955                 960 


Ala Gly Asn Val Ser Asn Phe Arg Gln Thr Tyr Glu Leu Leu Lys Val 
                965                 970                 975     


Arg Glu Met Asn Asp Leu His His Ala Lys Asp Ala Tyr Leu Asn Ile 
            980                 985                 990         


Val Val Gly Asn Ala Tyr Phe Val  Lys Phe Thr Lys Asn  Ala Ala Trp 
        995                 1000                 1005             


Phe Ile  Arg Asn Asn Pro Gly  Arg Ser Tyr Asn Leu  Lys Arg Met 
    1010                 1015                 1020             


Phe Glu  Phe Asp Ile Glu Arg  Ser Gly Glu Ile Ala  Trp Lys Ala 
    1025                 1030                 1035             


Gly Asn  Lys Gly Ser Ile Val  Thr Val Lys Lys Val  Met Gln Lys 
    1040                 1045                 1050             


Asn Asn  Ile Leu Val Thr Arg  Lys Ala Tyr Glu Val  Lys Gly Gly 
    1055                 1060                 1065             


Leu Phe  Asp Gln Gln Ile Met  Lys Lys Gly Lys Gly  Gln Val Pro 
    1070                 1075                 1080             


Ile Lys  Gly Asn Asp Glu Arg  Leu Ala Asp Ile Glu  Lys Tyr Gly 
    1085                 1090                 1095             


Gly Tyr  Asn Lys Ala Ala Gly  Thr Tyr Phe Met Leu  Val Lys Ser 
    1100                 1105                 1110             


Leu Asp  Lys Lys Gly Lys Glu  Ile Arg Thr Ile Glu  Phe Val Pro 
    1115                 1120                 1125             


Leu Tyr  Leu Lys Asn Gln Ile  Glu Ile Asn His Glu  Ser Ala Ile 
    1130                 1135                 1140             


Gln Tyr  Leu Ala Gln Glu Arg  Gly Leu Asn Ser Pro  Glu Ile Leu 
    1145                 1150                 1155             


Leu Ser  Lys Ile Lys Ile Asp  Thr Leu Phe Lys Val  Asp Gly Phe 
    1160                 1165                 1170             


Lys Met  Trp Leu Ser Gly Arg  Thr Gly Asn Gln Leu  Ile Phe Lys 
    1175                 1180                 1185             


Gly Ala  Asn Gln Leu Ile Leu  Ser His Gln Glu Ala  Ala Ile Leu 
    1190                 1195                 1200             


Lys Gly  Val Val Lys Tyr Val  Asn Arg Lys Asn Glu  Asn Lys Asp 
    1205                 1210                 1215             


Ala Lys  Leu Ser Glu Arg Asp  Gly Met Thr Glu Glu  Lys Leu Leu 
    1220                 1225                 1230             


Gln Leu  Tyr Asp Thr Phe Leu  Asp Lys Leu Ser Asn  Thr Val Tyr 
    1235                 1240                 1245             


Ser Ile  Arg Leu Ser Ala Gln  Ile Lys Thr Leu Thr  Glu Lys Arg 
    1250                 1255                 1260             


Ala Lys  Phe Ile Gly Leu Ser  Asn Glu Asp Gln Cys  Ile Val Leu 
    1265                 1270                 1275             


Asn Glu  Ile Leu His Met Phe  Gln Cys Gln Ser Gly  Ser Ala Asn 
    1280                 1285                 1290             


Leu Lys  Leu Ile Gly Gly Pro  Gly Ser Ala Gly Ile  Leu Val Met 
    1295                 1300                 1305             


Asn Asn  Asn Ile Thr Ala Cys  Lys Gln Ile Ser Val  Ile Asn Gln 
    1310                 1315                 1320             


Ser Pro  Thr Gly Ile Tyr Glu  Lys Glu Ile Asp Leu  Ile Lys Leu 
    1325                 1330                 1335             


<210>  863
<211>  1338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  863

Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Thr Asp Gly Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Ser His Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Lys Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Ala Asp Glu Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Asn Glu Ser Phe Leu Thr Asn Asp Asp Lys Glu 
            100                 105                 110         


Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Glu Ala His 
        115                 120                 125             


His His Lys Phe Pro Thr Ile Phe His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ser Thr Glu Lys Ser Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Gln Leu Lys Ala 
                165                 170                 175     


Glu Asn Thr Asn Val Gln Thr Leu Phe Asp Asp Phe Val Glu Val Tyr 
            180                 185                 190         


Asp Lys Thr Val Glu Glu Ser His Leu Ser Glu Ile Ser Val Ser Ser 
        195                 200                 205             


Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile 
    210                 215                 220                 


Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn Leu Ile 
225                 230                 235                 240 


Ala Leu Ser Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe Lys Leu 
                245                 250                 255     


Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu Glu Asp 
            260                 265                 270         


Leu Gly Glu Leu Leu Gly Lys Ile Gly Asp Asn Tyr Ala Asp Leu Phe 
        275                 280                 285             


Ile Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly Ile Leu 
    290                 295                 300                 


Thr Ile Asp Asp Asn Thr Thr Lys Ala Pro Leu Ser Ala Ser Met Ile 
305                 310                 315                 320 


Lys Arg Tyr Glu Glu His Gln Glu Asp Leu Ala Gln Leu Lys Lys Phe 
                325                 330                 335     


Ile Arg Gln Asn Leu Pro Asp Gln Tyr Ser Glu Val Phe Ser Asp Lys 
            340                 345                 350         


Thr Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn Gln Glu 
        355                 360                 365             


Ala Phe Tyr Lys Tyr Ile Lys Asn Met Leu Ser Lys Thr Glu Gly Ala 
    370                 375                 380                 


Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg Lys Gln 
385                 390                 395                 400 


Arg Thr Phe Asp Asn Gly Ser Val Pro His Gln Ile His Leu Gln Glu 
                405                 410                 415     


Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe Leu Lys 
            420                 425                 430         


Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp Ala Glu 
    450                 455                 460                 


Tyr Lys Ala Asp Lys Lys Val Thr Pro Trp Asn Phe Asp Asp Ile Leu 
465                 470                 475                 480 


Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Thr Arg Met Thr Leu Asn 
                485                 490                 495     


Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser Leu Val 
            500                 505                 510         


Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val 
        515                 520                 525             


Asn Glu Gln Gly Lys Ala Ile Phe Phe Asp Ala Asn Met Lys Gln Glu 
    530                 535                 540                 


Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys Asp Lys 
545                 550                 555                 560 


Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile Val Asn 
                565                 570                 575     


Leu Thr Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ser Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Arg Lys Ile Leu Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             


Lys Val Asn Glu Lys Ile Ile Glu Asp Ile Ile Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 


Ile Phe Thr Thr Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         


Gln Ser Asn Lys Thr Ile Leu Gly Tyr Leu Ile Asp Asp Gly Tyr Ser 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Pro Phe Lys 
    690                 695                 700                 


Glu Glu Ile Ala Arg Ala Gln Val Ile Gly Glu Thr Asp Asp Leu Asn 
705                 710                 715                 720 


Gln Leu Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         


Asn Pro Ala Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Arg Arg Ser Ser Gln Gln Arg Tyr Lys Arg Leu Glu Glu 
    770                 775                 780                 


Ala Ile Lys Asn Leu Asp His Asp Leu Asn His Lys Ile Leu Lys Glu 
785                 790                 795                 800 


His Pro Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Phe Leu Tyr 
                805                 810                 815     


Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Glu Asp Pro Leu Asp Ile 
            820                 825                 830         


Asn Arg Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe 
        835                 840                 845             


Ile Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Val Ser Ser Ala Lys 
    850                 855                 860                 


Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Asp Val Val Asn Arg 
865                 870                 875                 880 


Met Arg Pro Phe Trp Asn Lys Leu Leu Ser Cys Gly Leu Ile Ser Gln 
                885                 890                 895     


Arg Lys Tyr Ser Asn Leu Thr Lys Lys Glu Leu Lys Pro Asp Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Lys Arg Asp Glu 
    930                 935                 940                 


Asn Lys Lys Val Ile Arg Asp Val Lys Ile Ile Thr Leu Lys Ser Asn 
945                 950                 955                 960 


Leu Val Ser Gln Phe Arg Lys Asp Phe Lys Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Ile 
            980                 985                 990         


Gly Lys Ala Leu Leu Asp Val Tyr  Pro Gln Leu Glu Pro  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Glu Tyr Pro His Phe  His Gly Tyr Lys Glu  Asn Lys Ala 
    1010                 1015                 1020             


Thr Ala  Lys Lys Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1025                 1030                 1035             


Lys Asp  Asp Ile Arg Thr Asp  Glu Asn Gly Glu Ile  Val Trp Lys 
    1040                 1045                 1050             


Lys Asp  Glu His Ile Ser Asn  Ile Lys Arg Val Leu  Ser Tyr Pro 
    1055                 1060                 1065             


Gln Val  Asn Ile Val Lys Lys  Val Glu Ile Gln Thr  Val Gly Gln 
    1070                 1075                 1080             


Asn Gly  Gly Leu Phe Asp Asp  Asn Pro Lys Ser Pro  Leu Glu Val 
    1085                 1090                 1095             


Thr Pro  Ser Lys Leu Val Pro  Leu Lys Lys Glu Leu  Asn Pro Lys 
    1100                 1105                 1110             


Lys Tyr  Gly Gly Tyr Gln Lys  Pro Thr Thr Ala Tyr  Pro Val Leu 
    1115                 1120                 1125             


Leu Ile  Thr Asp Thr Lys Gln  Leu Ile Pro Ile Ser  Val Met Asn 
    1130                 1135                 1140             


Lys Lys  Gln Phe Glu Gln Asn  Pro Val Lys Phe Leu  Arg Asp Arg 
    1145                 1150                 1155             


Gly Tyr  Gln Gln Val Gly Lys  Asn Asp Phe Ile Lys  Leu Pro Lys 
    1160                 1165                 1170             


Tyr Thr  Leu Val Asp Ile Gly  Asp Gly Ile Lys Arg  Leu Trp Ala 
    1175                 1180                 1185             


Ser Ser  Lys Glu Ile His Lys  Gly Asn Gln Leu Val  Val Ser Lys 
    1190                 1195                 1200             


Lys Ser  Gln Ile Leu Leu Tyr  His Ala His His Leu  Asp Ser Asp 
    1205                 1210                 1215             


Leu Ser  Asn Asp Tyr Leu Gln  Asn His Asn Gln Gln  Phe Asp Val 
    1220                 1225                 1230             


Leu Phe  Asn Glu Ile Ile Ser  Phe Ser Lys Lys Cys  Lys Leu Gly 
    1235                 1240                 1245             


Lys Glu  His Ile Gln Lys Ile  Glu Asn Val Tyr Ser  Asn Lys Lys 
    1250                 1255                 1260             


Asn Ser  Ala Ser Ile Glu Glu  Leu Ala Glu Ser Phe  Ile Lys Leu 
    1265                 1270                 1275             


Leu Gly  Phe Thr Gln Leu Gly  Ala Thr Ser Pro Phe  Asn Phe Leu 
    1280                 1285                 1290             


Gly Val  Lys Leu Asn Gln Lys  Gln Tyr Lys Gly Lys  Lys Asp Tyr 
    1295                 1300                 1305             


Ile Leu  Pro Cys Thr Glu Gly  Thr Leu Ile Arg Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Thr Arg Val  Asp Leu Ser Lys Ile  Gly Glu Asp 
    1325                 1330                 1335             


<210>  864
<211>  1338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  864

Met Arg Asp Tyr Tyr Ile Gly Leu Asp Leu Gly Thr Gly Ser Leu Gly 
1               5                   10                  15      


Trp Ala Val Thr Asp Arg Glu Tyr Glu Ile Met Arg Ala His Gly Lys 
            20                  25                  30          


Ala Leu Trp Gly Val Arg Leu Phe Asp Ser Ala Asn Thr Ala Glu Glu 
        35                  40                  45              


Arg Arg Gly Phe Arg Thr Ala Arg Arg Arg Leu Asp Arg Arg Asn Trp 
    50                  55                  60                  


Arg Ile Glu Leu Leu Gln Glu Leu Phe Gly Glu Asp Ile Gly Lys Val 
65                  70                  75                  80  


Asp Ser Gly Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Met Pro Glu 
                85                  90                  95      


Asp Lys Arg Asp Val Asn Gly Asn Cys Pro Lys Leu Pro Tyr Ala Leu 
            100                 105                 110         


Phe Val Glu Asp Gly Tyr Thr Asp Lys Asp Tyr His Arg Gln Phe Pro 
        115                 120                 125             


Thr Ile Tyr His Leu Arg Lys Trp Leu Met Glu Thr Glu Glu Thr Pro 
    130                 135                 140                 


Asp Ile Arg Leu Val Tyr Leu Ala Leu His His Met Met Lys His Arg 
145                 150                 155                 160 


Gly His Phe Leu Phe Ser Gly Asn Ile Glu Lys Ile Lys Glu Phe Gln 
                165                 170                 175     


Glu Thr Phe Arg Gln Tyr Ile Gly Lys Ile Arg Glu Glu Glu Leu Asp 
            180                 185                 190         


Phe His Leu Cys Ile Glu Gly Glu Glu Leu Arg Glu Thr Glu Asn Ile 
        195                 200                 205             


Leu Lys Asp Lys Asn Leu Thr Arg Ser Ala Lys Lys Thr Arg Leu Ile 
    210                 215                 220                 


Lys Leu Leu Gly Ala His Thr Ala Cys Glu Lys Ala Ala Leu Asn Leu 
225                 230                 235                 240 


Val Ala Gly Gly Thr Val Lys Leu Ser Asp Ile Phe Gly Asn Ser Glu 
                245                 250                 255     


Leu Asp Ala Cys Glu Lys Pro Lys Leu Ser Phe Ala Asp Ala Gly Tyr 
            260                 265                 270         


Asp Asp Tyr Ala Gly Met Ile Glu Asp Glu Leu Gly Glu Gln His Val 
        275                 280                 285             


Ile Ile Glu Thr Ala Lys Ala Val Tyr Asp Trp Ser Val Leu Ala Asp 
    290                 295                 300                 


Ile Leu Gly Asp Tyr Arg Cys Ile Ser Glu Ala Lys Ala Ala Val Tyr 
305                 310                 315                 320 


Glu Lys His Gln Lys Asp Leu Arg His Leu Lys Glu Leu Val Lys Glu 
                325                 330                 335     


Asn Leu Gly Arg Asp Val Tyr Lys Glu Val Phe Val Lys Thr Asn Glu 
            340                 345                 350         


Lys Leu Pro Asn Tyr Ser Ala Tyr Ile Gly Met Thr Lys Lys Asn Gly 
        355                 360                 365             


Val Lys Ser Glu Met Glu Gly Lys Arg Cys Asp Arg Lys Ala Phe Tyr 
    370                 375                 380                 


Asp Tyr Leu Lys Lys Thr Val Val Asn Ala Ile Pro Asp Glu Ser Lys 
385                 390                 395                 400 


Thr Glu Tyr Leu Arg Lys Glu Met Glu Thr Glu Thr Phe Leu Pro Arg 
                405                 410                 415     


Gln Val Thr Lys Asp Asn Gly Val Ile Pro His Gln Val His Leu Gln 
            420                 425                 430         


Glu Leu Asp Ala Ile Leu Glu Asn Leu Ser Gly Arg Ile Pro Ala Leu 
        435                 440                 445             


Lys Glu Asn Gly Ser Lys Ile Arg Asp Ile Phe Thr Phe Arg Ile Pro 
    450                 455                 460                 


Tyr Tyr Val Gly Pro Leu Asn Gly Ile Val Lys Gly Gly Glu Arg Thr 
465                 470                 475                 480 


Asn Trp Val Arg Arg Lys Lys Ala Gly Arg Ile Cys Pro Trp Asn Phe 
                485                 490                 495     


Asp Glu Met Val Asp Thr Gly Ala Ser Ala Glu Glu Phe Ile Arg Arg 
            500                 505                 510         


Met Thr Ser Lys Cys Thr Tyr Leu Ile His Glu Asp Val Leu Pro Lys 
        515                 520                 525             


Asn Ser Met Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn 
    530                 535                 540                 


Val Arg Leu Asn Gly Glu Pro Ile Ser Val Glu Leu Lys Gln Lys Thr 
545                 550                 555                 560 


Tyr Glu Asp Leu Phe Gln Arg His Arg Lys Val Thr Arg Arg Arg Leu 
                565                 570                 575     


Thr Asp Tyr Ile Arg Arg Glu Gly Ile Ala Gly Arg Asp Ala Asp Ile 
            580                 585                 590         


Thr Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Thr Ala Tyr His Asp 
        595                 600                 605             


Phe Lys Glu Lys Leu Thr Gly Cys Glu Leu Ser Gln Ala Asp Lys Glu 
    610                 615                 620                 


Asn Ile Ile Leu Asn Ile Thr Leu Phe Gly Glu Asp Lys Ala Leu Leu 
625                 630                 635                 640 


Lys Lys Arg Leu Gly Ala Leu Tyr Pro Ala Leu Thr Glu Pro Gln Lys 
                645                 650                 655     


Lys Ala Ile Cys Ala Leu Ser Tyr Lys Gly Trp Gly Arg Leu Ser Gln 
            660                 665                 670         


Arg Leu Leu Glu Gly Ile Thr Ala Pro Ala Pro Glu Thr Gly Glu Ile 
        675                 680                 685             


Trp Thr Val Ile Arg Ala Met Trp Glu Thr Asn Asp Asn Leu Met Gln 
    690                 695                 700                 


Val Leu Ser Glu Lys Tyr Cys Phe Ala Ala Ala Ile Asp Glu Glu Asn 
705                 710                 715                 720 


Ala Gly Glu Glu Leu Lys Glu Ile Thr Tyr Lys Thr Val Glu Gln Met 
                725                 730                 735     


Asn Val Ser Pro Ala Val Arg Arg Gln Ile Trp Gln Ser Leu Gln Val 
            740                 745                 750         


Ile Lys Glu Ile Cys Lys Val Met Gly Gly Pro Pro Lys Arg Val Phe 
        755                 760                 765             


Val Glu Met Ala Arg Glu Lys Met Glu Ser Lys Arg Thr Glu Ser Arg 
    770                 775                 780                 


Lys Lys Arg Leu Ile Asp Leu Tyr Lys Lys Cys Arg Glu Glu Glu Arg 
785                 790                 795                 800 


Asp Trp Ile Glu Glu Leu Gly Asn Thr Glu Glu Thr Arg Leu Arg Ser 
                805                 810                 815     


Asp Lys Leu Tyr Leu Tyr Tyr Thr Gln Lys Gly Arg Cys Met Tyr Ser 
            820                 825                 830         


Gly Glu Val Ile Glu Leu Glu Glu Leu Trp Asp Asn Arg Lys Tyr Asp 
        835                 840                 845             


Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met Asp Asp Ser Leu Asp 
    850                 855                 860                 


Asn Arg Val Leu Val Lys Lys Glu Tyr Asn Ala Asp Lys Thr Asp Glu 
865                 870                 875                 880 


Tyr Pro Ile Arg Ala Asp Ile Arg Gly Lys Met Arg Ala Phe Trp Arg 
                885                 890                 895     


Ile Leu Arg Glu Glu Gly Phe Ile Ser Lys Glu Lys Tyr Asn Arg Leu 
            900                 905                 910         


Thr Arg Gly Thr Gly Phe Glu Pro Ser Glu Leu Ala Gly Phe Ile Ala 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Arg Gln Gly Thr Lys Ala Val Ala Ser Val 
    930                 935                 940                 


Leu Lys Gln Val Phe Pro Glu Thr Asp Ile Val Tyr Ala Lys Ala Arg 
945                 950                 955                 960 


Val Ala Ser Gln Phe Arg Gln Glu Phe Asp Leu Ile Lys Val Arg Glu 
                965                 970                 975     


Met Asn Asp Leu His His Ala Lys Asp Ala Tyr Val Asn Ile Val Val 
            980                 985                 990         


Gly Asn Val Tyr Tyr Thr Lys Phe  Thr Ser Asn Ala Ala  Trp Tyr Val 
        995                 1000                 1005             


Lys Glu  His Pro Gly Arg Ser  Tyr Asn Leu Lys Lys  Met Phe Thr 
    1010                 1015                 1020             


Ser Glu  Arg Asp Val Ala Arg  Asn Gly Glu Thr Ala  Trp Arg Ala 
    1025                 1030                 1035             


Gly Asn  Ser Gly Thr Ile Ala  Thr Val Lys Arg Val  Met Gly Lys 
    1040                 1045                 1050             


Asn Asn  Ile Leu Val Thr Arg  Arg Ser Tyr Glu Val  Lys Gly Gly 
    1055                 1060                 1065             


Leu Phe  Asp Gln Gln Leu Met  Lys Lys Gly Lys Gly  Gln Val Pro 
    1070                 1075                 1080             


Ile Lys  Gly Arg Asp Glu Arg  Leu Ala Asp Ile Asp  Lys Tyr Gly 
    1085                 1090                 1095             


Gly Tyr  Asn Lys Ala Ala Gly  Thr Tyr Phe Met Leu  Ala Glu Ser 
    1100                 1105                 1110             


Glu Asp  Lys Lys Gly Ala Lys  Ile Arg Ser Val Glu  Tyr Val Pro 
    1115                 1120                 1125             


Leu Tyr  Leu Cys Asn Cys Ile  Glu Lys Asp Glu Glu  Ala Ala Lys 
    1130                 1135                 1140             


Lys Tyr  Leu Gln Lys Glu Arg  Gly Leu Lys Asn Pro  Arg Val Leu 
    1145                 1150                 1155             


Ile Ala  Lys Ile Lys Ile Asp  Thr Leu Phe Lys Val  Asp Gly Phe 
    1160                 1165                 1170             


Tyr Met  Trp Leu Ser Gly Arg  Thr Gly Asn Gln Leu  Ile Phe Lys 
    1175                 1180                 1185             


Gly Ala  Asn Gln Leu Ile Leu  Ser Glu Pro Asp Met  Arg Ile Leu 
    1190                 1195                 1200             


Lys Lys  Val Leu Lys Tyr Val  Asn Arg Lys Lys Glu  Asn Lys Asn 
    1205                 1210                 1215             


Ala Val  Leu Gly Glu His Asp  Gln Leu Pro Glu Thr  Asp Leu Ile 
    1220                 1225                 1230             


Arg Leu  Tyr Asp Val Phe Leu  Asp Lys Ile Glu Asn  Thr Val Tyr 
    1235                 1240                 1245             


His Val  Arg Leu Ser Ala Gln  Gln Gly Thr Leu Thr  Lys Asn Lys 
    1250                 1255                 1260             


Asp Thr  Phe Cys Glu Leu Ser  Asn Glu Asp Lys Cys  Ile Val Leu 
    1265                 1270                 1275             


Ser Glu  Ile Leu His Met Phe  Gln Cys Gln Ser Gly  Ser Ala Asn 
    1280                 1285                 1290             


Leu Lys  Leu Ile Lys Gly Pro  Gly Ser Ala Gly Ile  Leu Val Leu 
    1295                 1300                 1305             


Asn Asn  Ile Ile Ser Lys Cys  Asn Gln Val Ser Ile  Ile His Gln 
    1310                 1315                 1320             


Ser Pro  Thr Gly Ile Tyr Glu  Gln Glu Ile Asp Leu  Lys Lys Ile 
    1325                 1330                 1335             


<210>  865
<211>  1339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  865

Met Asn Lys Ala Tyr Thr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Thr Asp Asp Tyr Arg Leu Met Ala Lys Lys Met 
            20                  25                  30          


Pro Val His Ser Lys Met Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Glu Arg Arg Asn 
    50                  55                  60                  


Lys Arg Ala Thr Arg Arg Arg Leu Arg Arg Arg Lys Tyr Arg Ile Leu 
65                  70                  75                  80  


Glu Leu Gln Lys Ile Phe Ser Glu Glu Ile Leu Lys Lys Asp Ser His 
                85                  90                  95      


Phe Phe Ala Arg Leu Asp Glu Ser Phe Leu Ile Pro Glu Asp Lys Gln 
            100                 105                 110         


Tyr Ala Arg Phe Pro Ile Phe Pro Thr Leu Leu Glu Glu Lys Ala Tyr 
        115                 120                 125             


Tyr Gln Asn Tyr Pro Thr Ile Tyr His Leu Arg Gln Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     


Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Glu Phe Ile Asp Ile Tyr 
            180                 185                 190         


Asn Glu Gln Phe Glu Glu Gly Ile Ile Phe Tyr Lys Asp Ile Pro Leu 
        195                 200                 205             


Ile Leu Thr Asp Lys Leu Ser Lys Ser Lys Lys Val Glu Lys Ile Leu 
    210                 215                 220                 


Gln Tyr Tyr Pro Lys Glu Lys Thr Thr Gly Cys Leu Ala Gln Phe Leu 
225                 230                 235                 240 


Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Gln Ala Phe His Leu 
                245                 250                 255     


Asp Glu Glu Val Lys Ile Gln Ile Ser Lys Glu Thr Tyr Glu Glu Asp 
            260                 265                 270         


Leu Glu Lys Leu Leu Arg Lys Ser Asn Glu Glu Met Ile Asp Val Phe 
        275                 280                 285             


Leu Gln Val Lys Lys Val Tyr Asp Ala Ile Leu Leu Ser Asp Ile Leu 
    290                 295                 300                 


Ser Thr Lys Met Lys Asp Thr Lys Ala Lys Leu Ser Ala Gly Met Ile 
305                 310                 315                 320 


Glu Arg Tyr Gln Asn His Lys Lys Asp Leu Glu Glu Leu Lys Gln Phe 
                325                 330                 335     


Val Arg Ala His Leu His Glu Lys Val Thr Val Phe Phe Lys Asp Ser 
            340                 345                 350         


Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr Gln Ala 
        355                 360                 365             


Asp Phe Tyr Lys Phe Leu Lys Lys Glu Leu Thr Gly Val Pro Gly Ser 
    370                 375                 380                 


Glu Pro Met Leu Ala Lys Ile Asp Gln Glu Asn Phe Leu Leu Lys Gln 
385                 390                 395                 400 


Arg Thr Pro Thr Asn Gly Val Ile Pro His Gln Val His Leu Thr Glu 
                405                 410                 415     


Phe Lys Ala Ile Ile Asp Gln Gln Lys Gln Tyr Tyr Pro Phe Leu Glu 
            420                 425                 430         


Lys Ser Lys Glu Lys Met Ile Gln Leu Leu Thr Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Leu Ala Gln Asp Lys Glu Thr Ser Ser Phe Ala Trp 
    450                 455                 460                 


Leu Glu Arg Lys Thr Thr Glu Lys Ile Lys Pro Trp Asn Ala Lys Asp 
465                 470                 475                 480 


Val Ile Asp Tyr Gly Ala Ser Ala Thr Lys Phe Ile Gln Arg Met Ile 
                485                 490                 495     


Asn Tyr Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys Tyr Ser 
            500                 505                 510         


Met Leu Tyr Gln Lys Tyr Thr Ile Phe Asn Glu Leu Thr Lys Val Ala 
        515                 520                 525             


Tyr Lys Asp Asp Arg Gly Ile Lys His Gln Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 


Leu Arg Ile Phe Gln Glu Leu Phe Lys Lys Gln Arg Arg Val Thr Lys 
545                 550                 555                 560 


Lys Lys Leu Gln His Phe Leu Ser Ala Asn Tyr Asn Ile Glu Asp Ala 
                565                 570                 575     


Glu Ile Leu Gly Val Asp Lys Val Phe Asn Ser Ser Tyr Ala Thr Tyr 
            580                 585                 590         


His Asp Phe Leu Glu Leu Ala Lys Pro Tyr Thr Glu Lys Ile Ile Glu 
        595                 600                 605             


Leu Leu Glu Gln Pro Glu Met Glu Glu Met Phe Glu Asp Ile Val Lys 
    610                 615                 620                 


Ile Ile Thr Ile Phe Glu Asp Arg Glu Met Val Arg Thr Gln Leu Lys 
625                 630                 635                 640 


Lys Tyr Gln Arg Ile Leu Gly Glu Glu Ile Phe Lys Lys Leu Val Lys 
                645                 650                 655     


Lys Lys Tyr Thr Gly Trp Gly Arg Leu Ser Lys Arg Leu Ile Asn Gly 
            660                 665                 670         


Ile Arg Asp Gln Lys Thr Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn 
        675                 680                 685             


Asp Asp Asp Phe Pro Tyr Asn Arg Asn Arg Asn Phe Met Gln Leu Ile 
    690                 695                 700                 


Asn Asp Asp His Leu Ser Phe Lys Glu Glu Ile Ala Lys Glu Leu Thr 
705                 710                 715                 720 


Leu Ser Asp Lys Gln Ser Leu Leu Glu Val Val Glu Ala Ile Pro Gly 
                725                 730                 735     


Ser Pro Ala Ile Lys Lys Gly Ile Trp Gln Thr Leu Lys Ile Val Glu 
            740                 745                 750         


Glu Leu Ile Ala Ile Ile Gly Tyr Lys Pro Lys Asn Ile Val Ile Glu 
        755                 760                 765             


Met Ala Arg Glu Asn Gln Thr Thr Thr Gly Gly Lys Asn Arg Ser Lys 
    770                 775                 780                 


Pro Arg Leu Lys Ser Leu Glu Glu Ala Leu Lys Asn Phe Asp Ser Gln 
785                 790                 795                 800 


Leu Leu Lys Glu Arg Pro Val Asp Asn Gln Ser Leu Gln Lys Asp Arg 
                805                 810                 815     


Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu 
            820                 825                 830         


Ser Leu Asp Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Ile 
        835                 840                 845             


Pro Arg Ser Phe Ile Val Asp His Ser Leu Asp Asn Lys Val Leu Val 
    850                 855                 860                 


Ser Ser Lys Glu Asn Arg Leu Lys Lys Asp Asp Val Pro Asp Ser Lys 
865                 870                 875                 880 


Val Val Lys Arg Met Lys Ala Tyr Trp Glu Lys Leu Leu Arg Ala Asn 
                885                 890                 895     


Leu Ile Ser Glu Arg Lys Phe Ser Tyr Leu Thr Lys Leu Glu Leu Thr 
            900                 905                 910         


Asp Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Ala Ile Leu His Gln Tyr Phe Asn Gln 
    930                 935                 940                 


Thr Gln Glu Leu Glu Lys Glu Lys Asp Ile Arg Ile Ile Thr Leu Lys 
945                 950                 955                 960 


Ser Ser Leu Val Ser Gln Phe Arg Gln Val Phe Gly Ile His Lys Val 
                965                 970                 975     


Arg Glu Ile Asn His His His His Ala His Asp Ala Tyr Leu Asn Ala 
            980                 985                 990         


Val Val Ala Leu Ala Leu Leu Lys  Lys Tyr Pro Arg Leu  Ala Pro Glu 
        995                 1000                 1005             


Phe Val  Tyr Gly Ser Phe Ala  Lys Phe His Leu Val  Lys Glu Asn 
    1010                 1015                 1020             


Lys Ala  Thr Ala Lys Lys Glu  Phe Tyr Ser Asn Ile  Leu Lys Phe 
    1025                 1030                 1035             


Phe Glu  Lys Glu Glu Gln Phe  Cys Asp Glu Asn Gly  Glu Ile Phe 
    1040                 1045                 1050             


Trp Asp  Lys Arg Lys His Ile  Gln Gln Ile Lys Lys  Val Ile Ser 
    1055                 1060                 1065             


Ser His  Gln Val Asn Ile Val  Lys Lys Val Glu Val  Gln Thr Gly 
    1070                 1075                 1080             


Ser Phe  Tyr Lys Glu Thr Val  Asn Thr Lys Glu Lys  Pro Asp Lys 
    1085                 1090                 1095             


Leu Ile  Lys Arg Lys Asn Asn  Trp Asp Val Thr Lys  Tyr Gly Gly 
    1100                 1105                 1110             


Phe Gly  Ser Pro Val Val Ala  Tyr Ala Val Val Phe  Thr Tyr Glu 
    1115                 1120                 1125             


Lys Gly  Lys Asn His Lys Lys  Ala Lys Ala Ile Glu  Gly Ile Thr 
    1130                 1135                 1140             


Ile Met  Glu Gln Ala Leu Phe  Glu Lys Asp Pro Ile  Ser Phe Leu 
    1145                 1150                 1155             


Ile Glu  Lys Gly Tyr Ser Asn  Val Asn Gln Phe Ile  Lys Leu Pro 
    1160                 1165                 1170             


Lys Tyr  Thr Leu Phe Glu Leu  Ala Asn Gly Gln Arg  Arg Met Leu 
    1175                 1180                 1185             


Ala Ser  His Gln Glu Leu Gln  Lys Ala Asn Ser Phe  Ile Leu Pro 
    1190                 1195                 1200             


Glu Lys  Leu Val Thr Leu Leu  Tyr His Ala Asn His  Tyr Asp Glu 
    1205                 1210                 1215             


Ile Ala  Tyr Lys Asp Ser Tyr  Asp Tyr Val Asn Glu  His Phe Ser 
    1220                 1225                 1230             


Asn Phe  Gln Asp Ile Leu Asp  Lys Val Ile Ile Phe  Ala Glu Lys 
    1235                 1240                 1245             


Tyr Thr  Ser Ala Pro Gln Lys  Leu Asn Gln Ile Ile  Ala Thr Tyr 
    1250                 1255                 1260             


Glu Lys  Asn Gln Glu Ala Asp  Arg Lys Ile Met Ala  His Ser Phe 
    1265                 1270                 1275             


Val Asn  Leu Met Gln Phe Asn  Ala Leu Gly Ala Pro  Ala Asp Phe 
    1280                 1285                 1290             


Lys Phe  Phe Asp Thr Thr Ile  Thr Arg Lys Arg Tyr  Thr Ser Leu 
    1295                 1300                 1305             


Thr Glu  Ile Trp Gln Ser Thr  Ile Ile Tyr Gln Ser  Val Thr Gly 
    1310                 1315                 1320             


Leu Tyr  Glu Thr Arg Arg Arg  Met Ala Asp Leu Trp  Asp Gly Val 
    1325                 1330                 1335             


Gln 
    


<210>  866
<211>  1339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  866

Met Glu Arg Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile Gly 
1               5                   10                  15      


Trp Ala Val Ile Gly Asp Asp Phe Lys Ile Lys Lys Lys Lys Gly Lys 
            20                  25                  30          


Asn Leu Ile Gly Thr Arg Leu Phe Asn Glu Gly Ala Thr Ala Ala Glu 
        35                  40                  45              


Arg Arg Gly Phe Arg Thr Gln Arg Arg Arg Leu Asn Arg Arg Lys Trp 
    50                  55                  60                  


Arg Leu Lys Leu Leu Glu Glu Ile Phe Asp Pro Tyr Met Ala Glu Val 
65                  70                  75                  80  


Asp Glu Tyr Phe Phe Ala Arg Leu Lys Ser Ser Asn Leu Ser Pro Lys 
                85                  90                  95      


Asp Ser Asn Lys Lys Tyr Leu Gly Ser Leu Leu Phe Pro Asp Lys Ser 
            100                 105                 110         


Asp Ser Asn Phe Tyr Asp Lys Tyr Pro Thr Ile Tyr His Leu Arg Arg 
        115                 120                 125             


Asp Leu Met Glu Lys Asp Glu Lys Phe Asp Leu Arg Glu Ile Tyr Leu 
    130                 135                 140                 


Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Glu Lys Val 
145                 150                 155                 160 


Pro Ala Lys Asn Tyr Lys Asn Ser Gly Ala Ser Ile Gly Phe Leu Leu 
                165                 170                 175     


Glu Glu Val Asn Ser Leu Tyr Lys Asp Ile Ile Gly Asp Glu Ser Ile 
            180                 185                 190         


Ala Ile Leu Asn Ser Glu Lys Phe Glu Asp Val Glu Lys Ile Ile Leu 
        195                 200                 205             


Asp Glu Glu Thr Arg Asn Leu Asp Lys Gln Lys Ser Val Gly Lys Leu 
    210                 215                 220                 


Leu Val Glu Asp Lys Lys Lys Lys Asn Ile Val Thr Ala Phe Ser Lys 
225                 230                 235                 240 


Ala Ile Phe Gly Tyr Lys Phe Asn Ile Glu Asp Leu Leu Leu Ile Glu 
                245                 250                 255     


Asn Asp Glu Lys Asn Lys Leu Thr Phe Asn Asp Glu Asn Ile Asp Asp 
            260                 265                 270         


Ile Phe Asn Glu Leu Ser His Ser Leu Asp Asp Lys Gln Met Asp Leu 
        275                 280                 285             


Leu Thr Lys Thr Arg Glu Ile Tyr Phe Lys Phe Lys Leu Asn Met Ile 
    290                 295                 300                 


Val Pro Thr Gly Tyr Thr Leu Ser Glu Ser Met Ile Glu Lys Tyr Glu 
305                 310                 315                 320 


Met His Lys Ala His Leu Lys Met Tyr Lys Glu Phe Ile Asn Thr Leu 
                325                 330                 335     


Asn Thr Lys Asp Arg Lys Ile Leu Lys Asn Ala Tyr Ser Asp Tyr Ile 
            340                 345                 350         


Asn Thr Glu Lys Ala Lys Ala Ala Asn Ala Gln Glu Asn Phe Tyr Lys 
        355                 360                 365             


Thr Val Lys Lys Ile Ile Lys Asp Asn Asn Ser Asp Thr Ala Lys Lys 
    370                 375                 380                 


Ile Ile Val Leu Ile Asp Glu Gly Asn Phe Met Pro Lys Gln Arg Thr 
385                 390                 395                 400 


Gly Glu Asn Gly Val Ile Pro His Gln Leu His Gln Ile Glu Leu Asp 
                405                 410                 415     


Arg Ile Ile Glu Asn Gln Ala Lys Tyr Tyr Pro Trp Leu Ala Glu Ala 
            420                 425                 430         


Asn Pro Val Glu Lys Asn Arg Lys Phe Ala Lys Tyr Lys Leu Asp Glu 
        435                 440                 445             


Leu Val Thr Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Asp Lys 
    450                 455                 460                 


Thr Glu Ser Asn Lys Asn Glu Lys Glu Thr Lys Phe Ala Trp Met Val 
465                 470                 475                 480 


Arg Lys Ala Lys Gly Thr Ile Thr Pro Trp Asn Phe Glu Asn Leu Val 
                485                 490                 495     


Asp Arg Thr Glu Ser Ala Asn Arg Phe Ile Lys Arg Met Thr Ser Lys 
            500                 505                 510         


Asp Thr Tyr Ile Ile Gly Glu Asp Val Leu Pro Ala Ser Ser Leu Leu 
        515                 520                 525             


Tyr Glu Lys Phe Lys Val Leu Asn Glu Leu Asn Asn Ile Lys Val Asn 
    530                 535                 540                 


Glu Lys Lys Leu Asp Ile Glu Gln Lys Gln Asp Ile Tyr Leu Asn Leu 
545                 550                 555                 560 


Phe Thr Thr Ala Lys Asn Val Thr Lys Lys Ser Leu Ala Thr Tyr Leu 
                565                 570                 575     


Asn Cys Ser Ala Asp Ser Ile Ser Gly Leu Ser Asp Gly Glu Lys Phe 
            580                 585                 590         


Asn Ser Ser Leu Ser Ser Tyr Ile Asp Leu Lys Ala Ile Leu Gly Asn 
        595                 600                 605             


Ile Val Asp Asp Cys Asn Lys Asn Glu Asp Leu Glu Lys Ile Ile Glu 
    610                 615                 620                 


Tyr Ser Thr Val Phe Glu Asp Gly Asn Ile Tyr Lys Glu Lys Leu Ser 
625                 630                 635                 640 


Glu Ile Ser Trp Leu Thr Asp Glu Gln Ile Glu Lys Leu Ser Asn Ile 
                645                 650                 655     


His Phe Lys Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gln Ile 
            660                 665                 670         


Thr Asn Glu Asn Gly Glu Arg Ile Ile Asp Ala Leu Trp Asn Thr Ser 
        675                 680                 685             


Asn Asn Phe Met Gln Ile Ile Asn Asp Glu Ser Ile Gln Ala Lys Leu 
    690                 695                 700                 


Ala Glu Ile Asn Gly Lys Tyr Ala Gly Lys Ser Asp Leu Glu Asp Ile 
705                 710                 715                 720 


Leu Ser Glu Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln 
                725                 730                 735     


Val Met Lys Val Val Glu Asp Ile Glu Lys Ala Met Gln Cys Glu Pro 
            740                 745                 750         


Thr Ser Ile Ser Ile Glu Phe Thr Arg Lys Lys Gln Lys Ser Arg Leu 
        755                 760                 765             


Thr Asn Thr Arg Tyr Lys Lys Ile Ser Asp Val Tyr Glu Lys Ile Ala 
    770                 775                 780                 


Asp Glu Val Ile Ser Glu Tyr Glu Leu Arg Lys Leu Gln Ser Glu Leu 
785                 790                 795                 800 


Asp Ser Asn Ala Asn Asn Met Arg Asp Arg Tyr Tyr Leu Tyr Phe Met 
                805                 810                 815     


Gln Leu Gly Arg Asp Met Tyr Thr Gly Glu Lys Ile Asn Ile Asp Glu 
            820                 825                 830         


Leu His Gln Arg Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe Ile 
        835                 840                 845             


Lys Asp Asp Ser Ile Asn Asn Arg Val Leu Thr Arg Lys Asp Val Asn 
    850                 855                 860                 


Asn Lys Glu Lys Ser Asp Lys Thr Ala Ala Asp Leu Tyr Ala Val Glu 
865                 870                 875                 880 


Met Ser Asp Phe Trp Arg Lys Leu Arg Lys Gln Gly Leu Ile Thr Glu 
                885                 890                 895     


Gln Lys Tyr Lys Asn Leu Leu Thr Lys Ser Asp Ser Ile Asp Lys Tyr 
            900                 905                 910         


Ala Lys Lys Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Ser Gln Val 
        915                 920                 925             


Val Lys Leu Val Ala Asn Ile Leu Gln Asp Lys Tyr Ser Asp Thr Lys 
    930                 935                 940                 


Ile Ile Glu Val Arg Ala Lys Leu Asn Ser Asn Leu Arg Glu Glu Tyr 
945                 950                 955                 960 


Lys Leu Ile Lys Asn Arg Ala Val Asn Asp Tyr His His Ala Ile Asp 
                965                 970                 975     


Gly Tyr Leu Thr Thr Phe Ile Gly Gln Tyr Leu Tyr Lys Thr Tyr Pro 
            980                 985                 990         


Lys Leu Arg Ser Tyr Phe Val Tyr  Asn Asp Phe Lys Lys  Leu Asp Ser 
        995                 1000                 1005             


Asn Tyr  Leu Lys His Met Lys  Lys Phe Asn Phe Leu  Trp Gln Leu 
    1010                 1015                 1020             


Gln Asp  Asp Lys Thr Ile Asp  Ile Tyr Asp Asn Val  Asn Asn Lys 
    1025                 1030                 1035             


Phe Val  Leu Asn Val Pro Glu  Met Lys Lys Tyr Ile  Gln Lys Ile 
    1040                 1045                 1050             


Tyr Asn  Tyr Lys Tyr Met Leu  Val Ser Lys Glu Val  Thr Thr Lys 
    1055                 1060                 1065             


Asn Gly  Ala Phe Tyr Asp Gln  Thr Lys Tyr Asn Ala  Lys Thr Val 
    1070                 1075                 1080             


Asn Leu  Ile Pro Ile Lys Lys  Asn Lys Pro Thr Asn  Ile Tyr Gly 
    1085                 1090                 1095             


Gly Tyr  Lys Gly Lys Val Ser  Ser Tyr Met Met Leu  Val Lys Ile 
    1100                 1105                 1110             


Arg Lys  Lys Lys Glu Val Ile  Tyr Lys Phe Val Gly  Val Pro Arg 
    1115                 1120                 1125             


Leu Trp  Thr Asp Glu Leu Asp  Arg Leu Lys Asp Thr  Thr Glu Arg 
    1130                 1135                 1140             


Lys Ile  Leu Leu Asn Glu Ile  Ala Lys Asn Ser Leu  Ser Lys Thr 
    1145                 1150                 1155             


Glu Gln  Asp Phe Glu Val Ile  Leu Asp Lys Val Tyr  Tyr Gly Gln 
    1160                 1165                 1170             


Leu Ile  Ile Asp Gly Asn Gln  Lys Tyr Thr Leu Gly  Ser Ser Glu 
    1175                 1180                 1185             


Tyr Lys  Tyr Asn Ala Met Gln  Leu His Leu Ser Lys  His Ala Leu 
    1190                 1195                 1200             


Glu Val  Leu Ala Lys Glu Asn  Val Lys Asp Ala Glu  Ile Thr Asp 
    1205                 1210                 1215             


Lys Asp  Leu Val Ser Val Tyr  Glu Glu Ile Leu Ser  Val Val Asn 
    1220                 1225                 1230             


Lys Tyr  Phe Glu Leu Tyr Asp  Ile Asn Lys Phe Arg  Gln Lys Leu 
    1235                 1240                 1245             


Asn Glu  Gly Leu Glu Ile Phe  Arg Lys Leu Pro Val  Tyr Asn Val 
    1250                 1255                 1260             


Tyr Glu  Ser Asn Lys Ile Lys  Gln Val Gly Lys Phe  Glu Val Leu 
    1265                 1270                 1275             


Asn Arg  Ile Leu Met Gly Leu  His Ala Asn Ala Met  Ile Thr Asp 
    1280                 1285                 1290             


Leu Lys  Val Leu Gly Ile Lys  Thr Lys Leu Gly Gln  Met Gln Val 
    1295                 1300                 1305             


Asn Gly  Gly Ile Lys Leu Ser  Pro Asp Ala Lys Leu  Ile Tyr Gln 
    1310                 1315                 1320             


Ser Pro  Thr Gly Ile Phe Ser  Arg Ala Val Arg Val  Lys Asp Leu 
    1325                 1330                 1335             


Gly 
    


<210>  867
<211>  1339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  867

Met Ser Leu Asn Thr Asn Lys Lys Tyr Asn Ile Gly Leu Asp Ile Gly 
1               5                   10                  15      


Thr Thr Ser Val Gly Trp Ala Val Thr Asp Ala Asp Gly Gln Lys Leu 
            20                  25                  30          


Leu Lys Ala Lys Asn Lys Asn Leu Trp Gly Val Ser Leu Phe Glu Ala 
        35                  40                  45              


Gly Lys Thr Ala Ala Glu Arg Arg Gly Tyr Arg Ser Thr Arg Arg Arg 
    50                  55                  60                  


Leu Asn His Arg Lys Phe Arg Leu Arg Leu Leu Glu Asp Met Phe Glu 
65                  70                  75                  80  


Lys Glu Ile Leu Ser Lys Asp Pro Ser Phe Phe Ile Arg Leu Lys Glu 
                85                  90                  95      


Ala Phe Leu Ser Pro Lys Asp Glu Gln Lys Gln Phe Lys Gly Asn Ile 
            100                 105                 110         


Leu Phe Asn Asp Lys Asp Tyr Thr Asp Ala Asp Tyr Tyr Glu Gln Tyr 
        115                 120                 125             


Lys Thr Ile Tyr His Leu Arg Tyr Asp Leu Ile Ser Gln His Arg Gln 
    130                 135                 140                 


Phe Asp Ile Arg Glu Val Tyr Leu Ala Ile His His Leu Ile Lys Tyr 
145                 150                 155                 160 


Arg Gly His Phe Ile Tyr Glu Asp Gln Thr Phe Thr Thr Asp Gly Asn 
                165                 170                 175     


Gln Leu Gln His His Ile Lys Ala Ile Ile Thr Met Ile Asn Ser Ile 
            180                 185                 190         


Leu Asn Arg Ile Ile Ile Pro Glu Thr Ile Asp Ile Asn Val Phe Glu 
        195                 200                 205             


Lys Thr Leu Leu Asp Arg Thr Met Asn Arg Ser Ser Lys Val Lys Phe 
    210                 215                 220                 


Leu Ile Glu Leu Thr Gly Glu Asp Lys Gln Asp Lys Pro Leu Leu Lys 
225                 230                 235                 240 


Glu Leu Phe Asn Leu Ile Val Gly Leu Lys Ala Lys Pro Ala Ser Ile 
                245                 250                 255     


Phe Glu Gln Glu Asn Leu Ala Thr Ile Val Glu Thr Met Asn Met Ser 
            260                 265                 270         


Thr Glu Gln Val Gln Leu Asp Leu Leu Thr Leu Ala Asp Val Leu Ala 
        275                 280                 285             


Asp Glu Glu Tyr Asp Leu Leu Leu Thr Ala Gln Lys Ile Tyr Ser Ala 
    290                 295                 300                 


Ile Ile Leu Asp Glu Ser Met Asp Gly Tyr Glu Tyr Phe Ala Glu Ala 
305                 310                 315                 320 


Lys Lys Glu Ser Tyr Arg Lys His Gln Glu Glu Leu Val Leu Val Lys 
                325                 330                 335     


Lys Met Leu Lys Ser Asn Ala Ile Thr Asn Asp Glu Arg Ala Lys Phe 
            340                 345                 350         


Glu Tyr Phe Tyr Thr Asp Tyr Ile Gly Ala Lys Ser Ser Asn Tyr Glu 
        355                 360                 365             


Glu Ser Lys Ile Ile Lys Lys Gly Leu Ser Ala Ala Tyr Gly Lys Tyr 
    370                 375                 380                 


Ser Lys Glu Glu Arg Leu Phe Lys His Ile Glu Leu Leu Leu Ala Lys 
385                 390                 395                 400 


Glu Asn Val Leu Thr Thr Val Glu His Asp Leu Leu Glu Lys Asn Ile 
                405                 410                 415     


Thr Phe Ala Ser Leu Leu Pro Leu Gln Arg Ser Ser Arg Asn Ala Val 
            420                 425                 430         


Ile Pro Tyr Gln Val His Glu Lys Glu Leu Val Ala Ile Leu Glu Asn 
        435                 440                 445             


Gln Ala Thr Tyr Tyr Pro Phe Leu Leu Glu Gln Lys Asp Asn Ile His 
    450                 455                 460                 


Lys Leu Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Asp 
465                 470                 475                 480 


Gln Lys Asp Ser Glu Phe Ala Trp Met Val Arg Lys Gln Ala Gly Lys 
                485                 490                 495     


Ile Thr Pro Phe Asn Phe Glu Glu Met Val Asp Ile Asp Ala Ser Ser 
            500                 505                 510         


Glu Ala Phe Ile Lys Arg Met Thr Asn Lys Cys Thr Tyr Leu Ile His 
        515                 520                 525             


Glu Asp Val Ile Pro Lys His Ser Phe Ser Tyr Ala Lys Phe Glu Val 
    530                 535                 540                 


Leu Asn Glu Leu Asn Lys Ile Arg Leu Asp Gly Lys Pro Ile Asp Ile 
545                 550                 555                 560 


Pro Leu Lys Lys Arg Ile Phe Glu Gly Leu Phe Leu Glu Lys Thr Lys 
                565                 570                 575     


Val Thr His Thr Ser Leu Lys Lys Trp Leu Ala Glu Tyr Glu His Met 
            580                 585                 590         


Thr Val Ser Val Val Gln Gly Thr Gln Lys Glu Thr Glu Phe Ala Thr 
        595                 600                 605             


Ser Leu Gln Ala Phe His Arg Phe Val Lys Ile Phe Asp Arg Glu Thr 
    610                 615                 620                 


Val Ser Asn Pro Ala Asn Glu Glu Met Phe Glu Lys Ile Ile Tyr Trp 
625                 630                 635                 640 


Ser Thr Val Phe Glu Asp Lys Lys Ile Met Arg Arg Lys Leu Ser Glu 
                645                 650                 655     


Tyr Pro Gln Leu Thr Glu Gln Gln Gln Val Gln Leu Ala Gln Val Arg 
            660                 665                 670         


Phe Arg Gly Trp Gly Arg Leu Ser Gln Arg Leu Ile Asn Arg Ile Lys 
        675                 680                 685             


Thr Pro Val Ser Gly Asp Glu Asp His Lys Leu Ser Ile Asn Glu Ile 
    690                 695                 700                 


Leu Trp Gln Thr Asn Glu Asn Phe Met Gln Ile Ile Arg Asn Lys Asp 
705                 710                 715                 720 


Tyr Leu Phe Lys Lys Ile Ile Glu Glu Gln Phe Glu Asn Glu Thr Ala 
                725                 730                 735     


Leu Leu Asn Lys Gln Arg Ile Asp Glu Leu Ala Thr Ser Pro Ala Asn 
            740                 745                 750         


Lys Lys Gly Ile Trp Gln Ala Ile Lys Ile Val Lys Glu Leu Glu Lys 
        755                 760                 765             


Val Leu Gln Gln Pro Ala Glu Asn Ile Phe Ile Glu Phe Ala Arg Ser 
    770                 775                 780                 


Asp Glu Glu Ser Lys Arg Ser Thr Pro Arg Asp Lys Phe Ile Glu Lys 
785                 790                 795                 800 


Ala Tyr Ala Gln Leu Lys Lys Glu Thr Asp Thr Phe Asn Leu Glu His 
                805                 810                 815     


Leu Lys Glu Leu Lys Gln Arg Ser Lys Gln Leu Ser Ser Gln Arg Leu 
            820                 825                 830         


Phe Leu Tyr Phe Ile Gln Asn Gly Lys Cys Met Tyr Ser Gly Glu His 
        835                 840                 845             


Leu Asp Ile Glu Arg Leu Asp Ser Tyr Glu Val Asp His Ile Leu Pro 
    850                 855                 860                 


Gln Ser Tyr Ile Lys Asp Asn Ser Ile Glu Asn Leu Ala Leu Val Lys 
865                 870                 875                 880 


Lys Val Glu Asn Gln Arg Lys Lys Asp Ser Leu Leu Leu Asn Ser Ser 
                885                 890                 895     


Ile Ile Asn Gln Asn Tyr Ser Arg Trp Glu Gln Leu Lys Asn Ala Gly 
            900                 905                 910         


Leu Ile Gly Glu Lys Lys Phe Arg Asn Leu Thr Arg Thr Lys Ile Thr 
        915                 920                 925             


Asp Arg Asp Lys Glu Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg 
    930                 935                 940                 


Gln Ile Thr Lys His Val Thr Gln Leu Leu Gln Gln Glu Tyr Lys Asp 
945                 950                 955                 960 


Thr Thr Lys Val Phe Ala Ile Lys Ala Thr Leu Val Ser Asp Leu Arg 
                965                 970                 975     


Arg Lys Phe Glu Phe Ile Lys Asn Arg Asn Val Asn Asp Tyr His His 
            980                 985                 990         


Ala Gln Asp Ala Phe Leu Val Ala  Phe Leu Gly Thr Asn  Ile Thr Ser 
        995                 1000                 1005             


Asn Tyr  Pro Lys Ile Glu Met  Glu Tyr Leu Phe Lys  Gly Tyr Gln 
    1010                 1015                 1020             


His Tyr  Leu Asn Glu Val Gly  Lys Ser Ala Ala Lys  Pro Lys Phe 
    1025                 1030                 1035             


Thr Phe  Ile Val Glu Asn Leu  Ser Lys Gln Gln Tyr  Asn Ser Thr 
    1040                 1045                 1050             


Thr Gly  Glu Val Lys Trp Asn  Pro Glu Val Asp Ile  Ala Lys Leu 
    1055                 1060                 1065             


Lys Arg  Ile Leu Asn Phe Lys  Gln Cys Asn Ile Val  Arg Lys Val 
    1070                 1075                 1080             


Glu Glu  Gln Ser Gly Ala Leu  Phe Lys Glu Thr Ile  Tyr Pro Val 
    1085                 1090                 1095             


Glu Glu  Ser Ser Ser Lys Thr  Ile Pro Leu Lys Lys  His Leu Asp 
    1100                 1105                 1110             


Thr Ala  Ile Tyr Gly Gly Tyr  Thr Ala Val Asn Tyr  Ala Ser Tyr 
    1115                 1120                 1125             


Ala Leu  Ile Gln Phe Lys Lys  Gly Arg Lys Leu Lys  Arg Glu Ile 
    1130                 1135                 1140             


Ile Gly  Ile Pro Leu Ala Val  Gln Thr Arg Ile Asp  Asn Ser Glu 
    1145                 1150                 1155             


Thr Ser  Leu Gln Thr Tyr Ile  Ala Glu Gln Ile Lys  Ser Glu Val 
    1160                 1165                 1170             


Glu Ile  Leu Asn Gly Arg Ile  Leu Lys Tyr Gln Leu  Ile Ser Asn 
    1175                 1180                 1185             


Asn Gly  Asn Arg Leu Tyr Ile  Ala Gly Pro Arg Glu  Arg His Asn 
    1190                 1195                 1200             


Ala Arg  Gln Leu Ile Val Ser  Asp Glu Ala Ala Lys  Val Ile Trp 
    1205                 1210                 1215             


Leu Ile  Ser Thr Lys Gln Ala  Val Glu Ala Thr Phe  Leu Lys His 
    1220                 1225                 1230             


Tyr Thr  Leu Glu His Leu Glu  Ala Val Phe Glu Glu  Leu Ile Arg 
    1235                 1240                 1245             


Lys Gln  Ala Ala Asp Tyr Gln  Ile Phe Glu Asn Leu  Ile Lys Lys 
    1250                 1255                 1260             


Ile Glu  Val Asn Lys Ala Tyr  Phe Tyr Ser Cys Thr  Tyr Asn Glu 
    1265                 1270                 1275             


Lys Val  Lys Val Ile Glu Glu  Leu Leu Lys Ile Thr  Gln Ala Asn 
    1280                 1285                 1290             


Ala Thr  Asn Gly Asp Leu Lys  Leu Leu Lys Met Thr  Asn Arg Glu 
    1295                 1300                 1305             


Gly Arg  Leu Gly Ser Val Ser  Val Ala Leu Asp Phe  Lys Ile Ile 
    1310                 1315                 1320             


Asn Gln  Ser Val Thr Gly Leu  Tyr Gln Ser Ile Glu  Asp Tyr Asn 
    1325                 1330                 1335             


Asn 
    


<210>  868
<211>  1339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  868

Met Ser Leu Asn Thr Asn Lys Lys Tyr Asn Ile Gly Leu Asp Ile Gly 
1               5                   10                  15      


Thr Thr Ser Val Gly Trp Ala Val Thr Asp Ala Asp Gly Gln Lys Leu 
            20                  25                  30          


Leu Lys Ala Lys Asn Lys Asn Leu Trp Gly Val Ser Leu Phe Glu Ala 
        35                  40                  45              


Gly Lys Thr Ala Ala Glu Arg Arg Gly Tyr Arg Ser Thr Arg Arg Arg 
    50                  55                  60                  


Leu Asn His Arg Lys Phe Arg Leu Arg Leu Leu Glu Asp Met Phe Glu 
65                  70                  75                  80  


Lys Glu Ile Leu Ser Lys Asp Pro Ser Phe Phe Ile Arg Leu Lys Glu 
                85                  90                  95      


Ala Phe Leu Ser Pro Lys Asp Glu Gln Lys Gln Phe Lys Gly Asn Ile 
            100                 105                 110         


Leu Phe Asn Asp Lys Asp Tyr Thr Asp Ala Asp Tyr Tyr Glu Gln Tyr 
        115                 120                 125             


Lys Thr Ile Tyr His Leu Arg Tyr Asp Leu Ile Ser Gln His Arg Gln 
    130                 135                 140                 


Phe Asp Ile Arg Glu Val Tyr Leu Ala Ile His His Leu Ile Lys Tyr 
145                 150                 155                 160 


Arg Gly His Phe Ile Tyr Glu Asp Gln Thr Phe Thr Thr Asp Gly Asn 
                165                 170                 175     


Gln Leu Gln His His Ile Lys Ala Ile Ile Thr Met Ile Asn Ser Ile 
            180                 185                 190         


Leu Asn Arg Ile Ile Ile Pro Glu Thr Ile Asp Ile Asn Val Phe Glu 
        195                 200                 205             


Lys Thr Leu Leu Asp Arg Thr Met Asn Arg Ser Ser Lys Val Lys Phe 
    210                 215                 220                 


Leu Ile Glu Leu Thr Gly Glu Asp Lys Gln Asp Lys Pro Leu Leu Lys 
225                 230                 235                 240 


Glu Leu Phe Asn Leu Ile Val Gly Leu Lys Ala Lys Pro Ala Ser Ile 
                245                 250                 255     


Phe Glu Gln Glu Asn Leu Ala Thr Ile Val Glu Thr Met Asn Met Ser 
            260                 265                 270         


Thr Glu Gln Val Gln Leu Asp Leu Leu Thr Leu Ala Asp Val Leu Ala 
        275                 280                 285             


Asp Glu Glu Tyr Asp Leu Leu Leu Thr Ala Gln Lys Ile Tyr Ser Ala 
    290                 295                 300                 


Ile Ile Leu Asp Glu Ser Met Asp Gly Tyr Glu Tyr Phe Ala Glu Ala 
305                 310                 315                 320 


Lys Lys Glu Ser Tyr Arg Lys His Gln Glu Glu Leu Val Leu Val Lys 
                325                 330                 335     


Lys Met Leu Lys Ser Asn Ala Ile Thr Asn Asp Glu Arg Ala Lys Phe 
            340                 345                 350         


Glu Tyr Phe Tyr Thr Asp Tyr Ile Gly Ala Lys Ser Ser Asn Tyr Glu 
        355                 360                 365             


Glu Ser Lys Ile Ile Lys Lys Gly Leu Ser Ala Ala Tyr Gly Lys Tyr 
    370                 375                 380                 


Ser Lys Glu Glu Arg Leu Phe Lys His Ile Glu Leu Leu Leu Ala Lys 
385                 390                 395                 400 


Glu Asn Val Leu Thr Thr Val Glu His Asp Leu Leu Glu Lys Asn Ile 
                405                 410                 415     


Thr Phe Ala Ser Leu Leu Pro Leu Gln Arg Ser Ser Arg Asn Ala Val 
            420                 425                 430         


Ile Pro Tyr Gln Val His Glu Lys Glu Leu Val Ala Ile Leu Glu Asn 
        435                 440                 445             


Gln Ala Thr Tyr Tyr Pro Phe Leu Leu Glu Gln Lys Asp Asn Ile His 
    450                 455                 460                 


Lys Leu Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Asp 
465                 470                 475                 480 


Gln Lys Asp Ser Glu Phe Ala Trp Met Val Arg Lys Gln Ala Gly Lys 
                485                 490                 495     


Ile Thr Pro Phe Asn Phe Glu Glu Met Val Asp Ile Asp Ala Ser Ser 
            500                 505                 510         


Glu Ala Phe Ile Lys Arg Met Thr Asn Lys Cys Thr Tyr Leu Ile His 
        515                 520                 525             


Glu Asp Val Ile Pro Lys His Ser Phe Ser Tyr Ala Lys Phe Glu Val 
    530                 535                 540                 


Leu Asn Glu Leu Asn Lys Ile Arg Leu Asp Gly Lys Pro Ile Asp Ile 
545                 550                 555                 560 


Pro Leu Lys Lys Arg Ile Phe Glu Gly Leu Phe Leu Glu Lys Thr Lys 
                565                 570                 575     


Val Thr His Thr Ser Leu Lys Lys Trp Leu Ala Glu His Glu His Met 
            580                 585                 590         


Thr Val Ser Val Val Gln Gly Thr Gln Lys Glu Thr Glu Phe Ala Thr 
        595                 600                 605             


Ser Leu Gln Ala Phe His Arg Phe Val Lys Ile Phe Asp Arg Glu Thr 
    610                 615                 620                 


Val Ser Asn Pro Ala Asn Glu Glu Met Phe Glu Lys Ile Ile Tyr Trp 
625                 630                 635                 640 


Ser Thr Val Phe Glu Asp Lys Lys Ile Met Arg Arg Lys Leu Ser Glu 
                645                 650                 655     


Tyr Pro Gln Leu Thr Glu Gln Gln Gln Val Gln Leu Ala Gln Val Arg 
            660                 665                 670         


Phe Arg Gly Trp Gly Arg Leu Ser Gln Arg Leu Ile Asn Arg Ile Lys 
        675                 680                 685             


Thr Pro Val Ser Gly Asp Glu Asp His Lys Leu Ser Ile Asn Glu Ile 
    690                 695                 700                 


Leu Trp Gln Thr Asn Glu Asn Phe Met Gln Ile Ile Arg Asn Lys Asp 
705                 710                 715                 720 


Tyr Leu Phe Lys Lys Ile Ile Glu Glu Gln Phe Glu Asn Glu Thr Ala 
                725                 730                 735     


Leu Leu Asn Lys Gln Arg Ile Asp Glu Leu Ala Thr Ser Pro Ala Asn 
            740                 745                 750         


Lys Lys Gly Ile Trp Gln Ala Ile Lys Ile Val Lys Glu Leu Glu Lys 
        755                 760                 765             


Val Leu Gln Gln Pro Ala Glu Asn Ile Phe Ile Glu Phe Ala Arg Ser 
    770                 775                 780                 


Asp Glu Glu Ser Lys Arg Ser Thr Pro Arg Asp Lys Phe Ile Glu Lys 
785                 790                 795                 800 


Ala Tyr Ala Gln Leu Lys Lys Glu Thr Asp Thr Phe Asn Leu Glu His 
                805                 810                 815     


Leu Lys Glu Leu Lys Gln Arg Ser Lys Gln Leu Ser Ser Gln Arg Leu 
            820                 825                 830         


Phe Leu Tyr Phe Ile Gln Asn Gly Lys Cys Met Tyr Ser Gly Glu His 
        835                 840                 845             


Leu Asp Ile Glu Arg Leu Asp Ser Tyr Glu Val Asp His Ile Leu Pro 
    850                 855                 860                 


Gln Ser Tyr Ile Lys Asp Asn Ser Ile Glu Asn Leu Ala Leu Val Lys 
865                 870                 875                 880 


Lys Val Glu Asn Gln Arg Lys Lys Asp Ser Leu Leu Leu Asn Ser Ser 
                885                 890                 895     


Ile Ile Asn Gln Asn Tyr Ser Arg Trp Glu Gln Leu Lys Asn Ala Gly 
            900                 905                 910         


Leu Ile Gly Glu Lys Lys Phe Arg Asn Leu Thr Arg Thr Lys Ile Thr 
        915                 920                 925             


Asp Arg Asp Lys Glu Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg 
    930                 935                 940                 


Gln Ile Thr Lys His Val Thr Gln Leu Leu Gln Gln Glu Tyr Lys Asp 
945                 950                 955                 960 


Thr Thr Lys Val Phe Ala Ile Lys Ala Thr Leu Val Ser Asp Leu Arg 
                965                 970                 975     


Arg Lys Phe Glu Phe Ile Lys Asn Arg Asn Val Asn Asp Tyr His His 
            980                 985                 990         


Ala Gln Asp Ala Phe Leu Val Ala  Phe Leu Gly Thr Asn  Ile Thr Ser 
        995                 1000                 1005             


Asn Tyr  Pro Lys Ile Glu Met  Glu Tyr Leu Phe Lys  Gly Tyr Gln 
    1010                 1015                 1020             


His Tyr  Leu Asn Glu Val Gly  Lys Ser Ala Ala Lys  Pro Lys Phe 
    1025                 1030                 1035             


Thr Phe  Ile Val Glu Asn Leu  Ser Lys Gln Gln Tyr  Asn Ser Thr 
    1040                 1045                 1050             


Thr Gly  Glu Val Lys Trp Asn  Pro Glu Val Asp Ile  Ala Lys Leu 
    1055                 1060                 1065             


Lys Arg  Ile Leu Asn Phe Lys  Gln Cys Asn Ile Val  Arg Lys Val 
    1070                 1075                 1080             


Glu Glu  Gln Ser Gly Ala Leu  Phe Lys Glu Thr Ile  Tyr Ser Val 
    1085                 1090                 1095             


Glu Glu  Ser Ser Ser Lys Thr  Ile Pro Leu Lys Lys  His Leu Asp 
    1100                 1105                 1110             


Thr Ala  Ile Tyr Gly Gly Tyr  Thr Ala Val Asn Tyr  Ala Ser Tyr 
    1115                 1120                 1125             


Ala Leu  Ile Gln Phe Lys Lys  Gly Arg Lys Leu Lys  Arg Glu Ile 
    1130                 1135                 1140             


Ile Gly  Ile Pro Leu Ala Val  Gln Thr Arg Ile Asp  Asn Ser Glu 
    1145                 1150                 1155             


Thr Ser  Leu Gln Thr Tyr Ile  Ala Glu Gln Ile Lys  Ser Glu Val 
    1160                 1165                 1170             


Glu Ile  Leu Asn Gly Arg Ile  Leu Lys Tyr Gln Leu  Ile Ser Asn 
    1175                 1180                 1185             


Asn Gly  Asn Arg Leu Tyr Ile  Ala Gly Pro Arg Glu  Arg His Asn 
    1190                 1195                 1200             


Ala Arg  Gln Leu Ile Val Ser  Asp Glu Ala Ala Lys  Val Ile Trp 
    1205                 1210                 1215             


Leu Ile  Ser Thr Lys Gln Ala  Val Glu Ala Thr Phe  Leu Lys His 
    1220                 1225                 1230             


Tyr Thr  Leu Glu His Leu Glu  Ala Val Phe Glu Glu  Leu Ile Arg 
    1235                 1240                 1245             


Lys Gln  Ala Ala Asp Tyr Gln  Ile Phe Glu Asn Leu  Ile Lys Lys 
    1250                 1255                 1260             


Ile Glu  Val Asn Lys Ala Tyr  Phe Tyr Ser Cys Thr  Tyr Asn Glu 
    1265                 1270                 1275             


Lys Val  Lys Val Ile Glu Glu  Leu Leu Lys Ile Thr  Gln Ala Asn 
    1280                 1285                 1290             


Ala Thr  Asn Gly Asp Leu Lys  Leu Leu Lys Met Thr  Asn Arg Glu 
    1295                 1300                 1305             


Gly Arg  Leu Gly Ser Val Ser  Val Ala Leu Asp Phe  Lys Ile Ile 
    1310                 1315                 1320             


Asn Gln  Ser Val Thr Gly Leu  Tyr Gln Ser Ile Glu  Asp Tyr Asn 
    1325                 1330                 1335             


Asn 
    


<210>  869
<211>  1339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  869

Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Val Leu Thr Asp Asp Tyr Arg Leu Val Ser Lys Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Thr Glu Lys Ser Ser Thr Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Leu Ala Arg Arg Arg Gln Arg Ile Leu 
65                  70                  75                  80  


Glu Leu Gln Lys Ile Phe Ala Pro Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      


Phe Phe Ala Arg Leu Asn Glu Ser Phe Leu Val Leu Asp Glu Lys Lys 
            100                 105                 110         


Gln Ser Arg His Pro Val Phe Ala Thr Ile Lys Gln Glu Lys Ser Tyr 
        115                 120                 125             


His Gln Thr Tyr Pro Thr Ile Tyr His Leu Arg Gln Ala Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Met Ala His 
145                 150                 155                 160 


Leu Leu Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     


Glu Asn Ser Ser Val Thr Glu Thr Phe Arg Gln Phe Leu Ser Thr Tyr 
            180                 185                 190         


Asn Gln Gln Phe Ser Glu Ala Asp Asp Lys Gln Thr Glu Lys Leu Asp 
        195                 200                 205             


Glu Ala Val Asp Cys Ser Phe Val Phe Thr Glu Lys Met Ser Lys Thr 
    210                 215                 220                 


Lys Lys Ala Glu Thr Leu Leu Lys Tyr Phe Pro His Glu Lys Ser Asn 
225                 230                 235                 240 


Gly Tyr Leu Ser Gln Phe Ile Lys Leu Met Val Gly Asn Gln Gly Asn 
                245                 250                 255     


Phe Lys Asn Val Phe Gly Leu Glu Glu Ala Lys Leu Gln Phe Ser Lys 
            260                 265                 270         


Glu Thr Tyr Glu Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly Asp 
        275                 280                 285             


Asp Tyr Ile Asp Leu Phe Val Gln Ala Lys Asn Val Tyr Asp Ala Val 
    290                 295                 300                 


Leu Leu Ser Glu Ile Leu Ser Asp Ser Thr Lys Asn Thr Arg Ala Lys 
305                 310                 315                 320 


Leu Ser Ala Gly Met Ile Arg Arg Tyr Asp Ala His Lys Glu Asp Leu 
                325                 330                 335     


Val Leu Leu Lys Arg Phe Val Lys Glu Asn Leu Pro Lys Lys Tyr Arg 
            340                 345                 350         


Ala Phe Phe Gly Asp Asn Ser Val Asn Gly Tyr Ala Gly Tyr Ile Glu 
        355                 360                 365             


Gly His Ala Thr Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu 
    370                 375                 380                 


Thr Gly Ile Arg Gly Ser Glu Val Phe Leu Thr Lys Ile Glu Gln Glu 
385                 390                 395                 400 


Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His 
                405                 410                 415     


Gln Ile His Leu Thr Glu Leu Arg Ala Ile Ile Ala Asn Gln Lys Lys 
            420                 425                 430         


His Tyr Pro Phe Leu Lys Glu Glu Gln Glu Lys Leu Glu Ser Leu Leu 
        435                 440                 445             


Thr Phe Lys Ile Pro Tyr Tyr Val Gly Pro Leu Ala Lys Lys Gln Glu 
    450                 455                 460                 


Asn Ser Pro Phe Ala Trp Leu Ile Arg Lys Ser Glu Glu Lys Ile Lys 
465                 470                 475                 480 


Pro Trp Asn Leu Pro Glu Ile Val Asp Met Glu Gly Ser Ala Val Arg 
                485                 490                 495     


Phe Ile Glu Arg Met Ile Asn Thr Asp Met Tyr Met Pro His Asn Lys 
            500                 505                 510         


Val Leu Pro Lys Asn Ser Leu Leu Tyr Gln Lys Phe Ser Ile Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Val Arg Tyr Gln Asp Glu Arg Gly Gln Met Asn Tyr 
    530                 535                 540                 


Phe Ser Ser Ile Glu Lys Lys Glu Ile Phe His Glu Leu Phe Glu Lys 
545                 550                 555                 560 


Asn Arg Lys Val Thr Lys Lys Asp Leu Gln Glu Phe Leu Tyr Leu Lys 
                565                 570                 575     


Tyr Asp Ile Lys His Ala Glu Leu Ser Gly Ile Glu Lys Ala Phe Asn 
            580                 585                 590         


Ala Ser Tyr Thr Thr Tyr His Asp Phe Leu Thr Met Ser Glu Asn Lys 
        595                 600                 605             


Arg Glu Met Lys Gln Trp Leu Glu Asp Pro Glu Leu Ala Ser Met Phe 
    610                 615                 620                 


Glu Glu Ile Ile Lys Thr Leu Thr Val Phe Glu Asp Arg Glu Met Ile 
625                 630                 635                 640 


Lys Thr Arg Leu Ser His His Glu Ala Thr Leu Gly Lys His Ile Ile 
                645                 650                 655     


Lys Lys Leu Thr Lys Lys His Tyr Thr Gly Trp Gly Arg Leu Ser Lys 
            660                 665                 670         


Glu Leu Ile Gln Gly Ile Arg Asp Lys Gln Ser Asn Lys Thr Ile Leu 
        675                 680                 685             


Asp Tyr Leu Ile Asn Asp Asp Asp Phe Pro His His Arg Asn Arg Asn 
    690                 695                 700                 


Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys Lys Glu Ile 
705                 710                 715                 720 


Lys Lys Ala Gln Met Ile Thr Asp Thr Glu Asn Leu Glu Glu Ile Val 
                725                 730                 735     


Lys Glu Leu Thr Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Ser 
            740                 745                 750         


Leu Lys Ile Val Asp Glu Ile Val Gly Ile Met Gly Tyr Glu Pro Ala 
        755                 760                 765             


Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr Gly Arg Gly 
    770                 775                 780                 


Leu Lys Ser Ser Arg Pro Arg Leu Lys Ala Leu Glu Glu Ser Leu Lys 
785                 790                 795                 800 


Asp Phe Gly Ser Gln Leu Leu Lys Glu Tyr Pro Thr Asp Asn Ser Ser 
                805                 810                 815     


Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg Asp 
            820                 825                 830         


Met Tyr Thr Gly Ala Pro Leu Asp Ile His Arg Leu Ser Asp Tyr Asp 
        835                 840                 845             


Ile Asp His Ile Ile Pro Arg Ser Phe Thr Thr Asp Asn Ser Ile Asp 
    850                 855                 860                 


Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg Leu Lys Lys Asp Asp 
865                 870                 875                 880 


Val Pro Ser Glu Lys Val Val Lys Lys Met Arg Ser Phe Trp Tyr Asp 
                885                 890                 895     


Leu Tyr Ser Ser Lys Leu Ile Ser Lys Arg Lys Leu Asp Asn Leu Thr 
            900                 905                 910         


Lys Ile Lys Leu Thr Glu Glu Asp Lys Ala Gly Phe Ile Lys Arg Gln 
        915                 920                 925             


Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gly Ile Leu His 
    930                 935                 940                 


His Arg Phe Asn Lys Ala Glu Asp Thr Asn Glu Pro Ile Arg Lys Val 
945                 950                 955                 960 


Arg Ile Ile Thr Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Asn Arg 
                965                 970                 975     


Phe Gly Ile Tyr Lys Val Arg Glu Ile Asn Glu Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Gly Val Val  Ala Leu Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Gln  Leu Ala Pro Glu Phe  Val Tyr Gly Glu Tyr  Leu Lys Phe 
    1010                 1015                 1020             


Asn Ala  His Lys Ala Asn Lys  Ala Thr Val Lys Lys  Glu Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Lys Phe Phe  Glu Ser Asp Thr Pro  Val Cys Asp 
    1040                 1045                 1050             


Glu Asn  Gly Glu Ile Phe Trp  Asp Lys Ser Lys Ser  Ile Ala Gln 
    1055                 1060                 1065             


Val Lys  Lys Val Ile Asn His  His His Met Asn Ile  Val Lys Lys 
    1070                 1075                 1080             


Thr Glu  Ile Gln Lys Gly Gly  Phe Ser Lys Glu Thr  Val Glu Pro 
    1085                 1090                 1095             


Lys Lys  Asp Ser Ser Lys Leu  Leu Pro Arg Lys Asn  Asn Trp Asp 
    1100                 1105                 1110             


Pro Ala  Lys Tyr Gly Gly Leu  Gly Ser Pro Asn Val  Ala Tyr Thr 
    1115                 1120                 1125             


Val Ala  Phe Thr Tyr Glu Lys  Gly Lys Ala Arg Lys  Arg Thr Asn 
    1130                 1135                 1140             


Ala Leu  Glu Gly Ile Thr Ile  Met Glu Arg Glu Ala  Phe Glu Gln 
    1145                 1150                 1155             


Ser Pro  Val Leu Phe Leu Lys  Asn Lys Gly Tyr Glu  Gln Ala Glu 
    1160                 1165                 1170             


Ile Glu  Met Lys Leu Pro Lys  Tyr Ala Leu Phe Glu  Leu Glu Asn 
    1175                 1180                 1185             


Gly Arg  Lys Arg Met Val Ala  Ser Asn Lys Glu Ala  Gln Lys Ala 
    1190                 1195                 1200             


Asn Ser  Phe Leu Leu Pro Glu  His Leu Val Thr Leu  Leu Tyr His 
    1205                 1210                 1215             


Ala Lys  Gln Tyr Asp Glu Ile  Ser His Lys Glu Ser  Phe Asp Tyr 
    1220                 1225                 1230             


Val Asn  Glu His His Lys Glu  Phe Ser Glu Val Phe  Ala Arg Val 
    1235                 1240                 1245             


Leu Glu  Phe Ala Gly Lys Tyr  Thr Leu Ala Glu Lys  Asn Ile Glu 
    1250                 1255                 1260             


Lys Leu  Glu Lys Ile Tyr Lys  Glu Asn Gln Thr Asp  Asp Leu Ala 
    1265                 1270                 1275             


Lys Leu  Ala Ser Ser Phe Val  Asn Leu Met Gln Phe  Asn Ala Met 
    1280                 1285                 1290             


Gly Ala  Pro Ala Asp Phe Lys  Phe Phe Asp Val Thr  Ile Pro Arg 
    1295                 1300                 1305             


Lys Arg  Tyr Thr Ser Leu Thr  Glu Ile Trp Gln Ser  Thr Ile Ile 
    1310                 1315                 1320             


His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr Arg Ile  Arg Met Gly 
    1325                 1330                 1335             


Lys 
    


<210>  870
<211>  1340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  870

Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Val Leu Thr Asp Asp Tyr Arg Leu Val Ser Lys Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Thr Glu Lys Ser Ser Thr Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Leu Ala Arg Arg Arg Gln Arg Ile Leu 
65                  70                  75                  80  


Glu Leu Gln Lys Ile Phe Ala Pro Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      


Phe Phe Ala Arg Leu Asn Glu Ser Phe Leu Val Leu Asp Glu Lys Lys 
            100                 105                 110         


Gln Ser Arg His Pro Val Phe Ala Thr Ile Lys Gln Glu Lys Ser Tyr 
        115                 120                 125             


His Gln Thr Tyr Pro Thr Ile Tyr His Leu Arg Gln Ala Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Met Ala His 
145                 150                 155                 160 


Leu Leu Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     


Glu Asn Ser Ser Val Thr Glu Thr Phe Arg Gln Phe Leu Ser Thr Tyr 
            180                 185                 190         


Asn Gln Gln Phe Ser Glu Ala Asp Asp Lys Gln Thr Glu Lys Leu Asp 
        195                 200                 205             


Glu Ala Val Asp Cys Ser Phe Val Phe Thr Glu Lys Met Ser Lys Thr 
    210                 215                 220                 


Lys Lys Ala Glu Thr Leu Leu Lys Tyr Phe Pro His Glu Lys Ser Asn 
225                 230                 235                 240 


Gly Tyr Leu Ser Gln Phe Ile Lys Leu Met Val Gly Asn Gln Gly Asn 
                245                 250                 255     


Phe Lys Asn Val Phe Gly Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser 
            260                 265                 270         


Lys Glu Thr Tyr Glu Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly 
        275                 280                 285             


Asp Asp Tyr Ile Asp Leu Phe Val Gln Ala Lys Asn Val Tyr Asp Ala 
    290                 295                 300                 


Val Leu Leu Ser Glu Ile Leu Ser Asp Ser Thr Lys Asn Thr Arg Ala 
305                 310                 315                 320 


Lys Leu Ser Ala Gly Met Ile Arg Arg Tyr Asp Ala His Lys Glu Asp 
                325                 330                 335     


Leu Val Leu Leu Lys Arg Phe Val Lys Glu Asn Leu Pro Lys Lys Tyr 
            340                 345                 350         


Arg Ala Phe Phe Gly Asp Asn Ser Val Asn Gly Tyr Ala Gly Tyr Ile 
        355                 360                 365             


Glu Gly His Ala Thr Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu 
    370                 375                 380                 


Leu Thr Gly Ile Arg Gly Ser Glu Val Phe Leu Thr Lys Ile Glu Gln 
385                 390                 395                 400 


Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro 
                405                 410                 415     


His Gln Ile His Leu Thr Glu Leu Arg Ala Ile Ile Ala Asn Gln Lys 
            420                 425                 430         


Lys His Tyr Pro Phe Leu Lys Glu Glu Gln Glu Lys Leu Glu Ser Leu 
        435                 440                 445             


Leu Thr Phe Lys Ile Pro Tyr Tyr Val Gly Pro Leu Ala Lys Lys Gln 
    450                 455                 460                 


Glu Asn Ser Pro Phe Ala Trp Leu Ile Arg Lys Ser Glu Glu Lys Ile 
465                 470                 475                 480 


Lys Pro Trp Asn Leu Pro Glu Ile Val Asp Met Glu Gly Ser Ala Val 
                485                 490                 495     


Arg Phe Ile Glu Arg Met Ile Asn Thr Asp Met Tyr Met Pro His Asn 
            500                 505                 510         


Lys Val Leu Pro Lys Asn Ser Leu Leu Tyr Gln Lys Phe Ser Ile Tyr 
        515                 520                 525             


Asn Glu Leu Thr Lys Val Arg Tyr Gln Asp Glu Arg Gly Gln Met Asn 
    530                 535                 540                 


Tyr Phe Ser Ser Ile Glu Lys Lys Glu Ile Phe His Glu Leu Phe Glu 
545                 550                 555                 560 


Lys Asn Arg Lys Val Thr Lys Lys Asp Leu Gln Glu Phe Leu Tyr Leu 
                565                 570                 575     


Lys Tyr Asp Ile Lys His Ala Glu Leu Ser Gly Ile Glu Lys Ala Phe 
            580                 585                 590         


Asn Ala Ser Tyr Thr Thr Tyr His Asp Phe Leu Thr Met Ser Glu Asn 
        595                 600                 605             


Lys Arg Glu Met Lys Gln Trp Leu Glu Asp Pro Glu Leu Ala Ser Met 
    610                 615                 620                 


Phe Glu Glu Ile Ile Lys Thr Leu Thr Val Phe Glu Asp Arg Glu Met 
625                 630                 635                 640 


Ile Lys Thr Arg Leu Ser His His Glu Ala Thr Leu Gly Lys His Ile 
                645                 650                 655     


Ile Lys Lys Leu Thr Lys Lys His Tyr Thr Gly Trp Gly Arg Leu Ser 
            660                 665                 670         


Lys Glu Leu Ile Gln Gly Ile Arg Asp Lys Gln Ser Asn Lys Thr Ile 
        675                 680                 685             


Leu Asp Tyr Leu Ile Asn Asp Asp Asp Phe Pro His His Arg Asn Arg 
    690                 695                 700                 


Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys Lys Glu 
705                 710                 715                 720 


Ile Lys Lys Ala Gln Met Ile Thr Asp Thr Glu Asn Leu Glu Glu Ile 
                725                 730                 735     


Val Lys Glu Leu Thr Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln 
            740                 745                 750         


Ser Leu Lys Ile Val Asp Glu Ile Val Gly Ile Met Gly Tyr Glu Pro 
        755                 760                 765             


Ala Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr Gly Arg 
    770                 775                 780                 


Gly Leu Lys Ser Ser Arg Pro Arg Leu Lys Ala Leu Glu Glu Ser Leu 
785                 790                 795                 800 


Lys Asp Phe Gly Ser Gln Leu Leu Lys Glu Tyr Pro Thr Asp Asn Ser 
                805                 810                 815     


Ser Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg 
            820                 825                 830         


Asp Met Tyr Thr Gly Ala Pro Leu Asp Ile His Arg Leu Ser Asp Tyr 
        835                 840                 845             


Asp Ile Asp His Ile Ile Pro Arg Ser Phe Thr Thr Asp Asn Ser Ile 
    850                 855                 860                 


Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg Leu Lys Lys Asp 
865                 870                 875                 880 


Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg Ser Phe Trp Tyr 
                885                 890                 895     


Asp Leu Tyr Ser Ser Lys Leu Ile Ser Lys Arg Lys Leu Asp Asn Leu 
            900                 905                 910         


Thr Lys Ile Lys Leu Thr Glu Glu Asp Lys Ala Gly Phe Ile Lys Arg 
        915                 920                 925             


Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gly Ile Leu 
    930                 935                 940                 


His His Arg Phe Asn Lys Ala Glu Asp Thr Asn Glu Pro Ile Arg Lys 
945                 950                 955                 960 


Val Arg Ile Ile Thr Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Asn 
                965                 970                 975     


Arg Phe Gly Ile Tyr Lys Val Arg Glu Ile Asn Glu Tyr His His Ala 
            980                 985                 990         


His Asp Ala Tyr Leu Asn Gly Val  Val Ala Leu Ala Leu  Leu Lys Lys 
        995                 1000                 1005             


Tyr Pro  Gln Leu Ala Pro Glu  Phe Val Tyr Gly Glu  Tyr Leu Lys 
    1010                 1015                 1020             


Phe Asn  Ala His Lys Ala Asn  Lys Ala Thr Val Lys  Lys Glu Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Lys Phe  Phe Glu Ser Asp Thr  Pro Val Cys 
    1040                 1045                 1050             


Asp Glu  Asn Gly Glu Ile Phe  Trp Asp Lys Ser Lys  Ser Ile Ala 
    1055                 1060                 1065             


Gln Val  Lys Lys Val Ile Asn  His His His Met Asn  Ile Val Lys 
    1070                 1075                 1080             


Lys Thr  Glu Ile Gln Lys Gly  Gly Phe Ser Lys Glu  Thr Val Glu 
    1085                 1090                 1095             


Pro Lys  Lys Asp Ser Ser Lys  Leu Leu Pro Arg Lys  Asn Asn Trp 
    1100                 1105                 1110             


Asp Pro  Ala Lys Tyr Gly Gly  Leu Gly Ser Pro Asn  Val Ala Tyr 
    1115                 1120                 1125             


Thr Val  Ala Phe Thr Tyr Glu  Lys Gly Lys Ala Arg  Lys Arg Thr 
    1130                 1135                 1140             


Asn Ala  Leu Glu Gly Ile Thr  Ile Met Glu Arg Glu  Ala Phe Glu 
    1145                 1150                 1155             


Gln Ser  Pro Val Leu Phe Leu  Lys Asn Lys Gly Tyr  Glu Gln Ala 
    1160                 1165                 1170             


Glu Ile  Glu Met Lys Leu Pro  Lys Tyr Ala Leu Phe  Glu Leu Glu 
    1175                 1180                 1185             


Asn Gly  Arg Lys Arg Met Val  Ala Ser Asn Lys Glu  Ala Gln Lys 
    1190                 1195                 1200             


Ala Asn  Ser Phe Leu Leu Pro  Glu His Leu Val Thr  Leu Leu Tyr 
    1205                 1210                 1215             


His Ala  Lys Gln Tyr Asp Glu  Ile Ser His Lys Glu  Ser Phe Asp 
    1220                 1225                 1230             


Tyr Val  Asn Glu His His Lys  Glu Phe Ser Glu Val  Phe Ala Arg 
    1235                 1240                 1245             


Val Leu  Glu Phe Ala Gly Lys  Tyr Thr Leu Ala Glu  Lys Asn Ile 
    1250                 1255                 1260             


Glu Lys  Leu Glu Lys Ile Tyr  Lys Glu Asn Gln Thr  Asp Asp Leu 
    1265                 1270                 1275             


Ala Lys  Leu Ala Ser Ser Phe  Val Asn Leu Met Gln  Phe Asn Ala 
    1280                 1285                 1290             


Met Gly  Ala Pro Ala Asp Phe  Lys Phe Phe Asp Val  Thr Ile Pro 
    1295                 1300                 1305             


Arg Lys  Arg Tyr Thr Ser Leu  Thr Glu Ile Trp Gln  Ser Thr Ile 
    1310                 1315                 1320             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Arg Met 
    1325                 1330                 1335             


Gly Lys  
    1340 


<210>  871
<211>  1340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  871

Met Lys Thr Asp Tyr Phe Met Gly Leu Asp Met Gly Thr Gly Ser Leu 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Ser Ser Tyr His Leu Cys Arg Ala His Gly 
            20                  25                  30          


Lys Asp Leu Trp Gly Val Arg Leu Phe Glu Thr Ala Gln Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Val Phe Arg Cys Asn Arg Arg Arg Leu Asp Arg Arg Lys 
    50                  55                  60                  


Arg Arg Ile Gln Leu Leu Gln Asp Ile Phe Ala Pro Glu Ile Tyr Lys 
65                  70                  75                  80  


Ile Asp Pro Gly Phe Phe Leu Arg Leu Lys Glu Ser Arg Tyr Leu Pro 
                85                  90                  95      


Glu Asp Lys Lys Asn Leu Glu Gly Lys Thr Pro Glu Leu Pro Tyr Ala 
            100                 105                 110         


Leu Phe Val Asp Lys Lys Tyr Thr Asp Lys Asp Phe His Lys Glu Tyr 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Leu Arg Leu Met Gln Asp Arg Thr Pro 
    130                 135                 140                 


Ala Asp Val Arg Leu Val Tyr Leu Ala Leu His His Leu Ile Lys His 
145                 150                 155                 160 


Arg Gly His Phe Leu Phe Ala Gly Ile Glu Ala Asp Lys Val Thr Asp 
                165                 170                 175     


Phe Ser Ala Ala Phe Ser Glu Phe Leu Arg Ile Val Gln Glu Gln Glu 
            180                 185                 190         


Leu Asp Ile Ser Phe Thr Pro Asp Glu Glu Ser Ser Gln Ile Ile Lys 
        195                 200                 205             


Glu Thr Leu Gln Asn Thr Asn Phe Thr Arg Ser Lys Lys Ser Thr Glu 
    210                 215                 220                 


Leu Ile Lys Gly Leu Lys Ala Ser Thr Gly Cys Glu Lys Ala Leu Leu 
225                 230                 235                 240 


Lys Leu Ile Ser Gly Cys Lys Val Lys Leu Ser Ala Val Phe Asp Asp 
                245                 250                 255     


Pro Gly Cys Asp Ala Tyr Glu Lys Pro Gln Val Cys Phe Ala Glu Ser 
            260                 265                 270         


Ser Tyr Glu Asp Tyr Ala Ala Ile Leu Glu Asp Glu Leu Glu Asp Arg 
        275                 280                 285             


Phe Ile Val Ile Ala Ala Ala Lys Ala Leu Tyr Asp Trp Ser Val Leu 
    290                 295                 300                 


Val Asn Ile Leu Gly Glu Ala Ser Thr Val Ser Glu Ala Lys Val Gly 
305                 310                 315                 320 


Ile Tyr Glu Lys His Arg Arg Asp Leu Lys Tyr Leu Lys His Leu Val 
                325                 330                 335     


Lys Thr Asn Leu Thr Arg Glu Asp Tyr Gln Asp Leu Phe Val Lys Ala 
            340                 345                 350         


Gly Ala Lys Lys Asn Asn Tyr Cys Ala Tyr Ile Gly Met Thr Lys Lys 
        355                 360                 365             


Asn Asn Lys Lys Val Val Leu Glu Glu Lys Arg Cys Thr Lys Glu Asp 
    370                 375                 380                 


Phe Tyr Ala Tyr Leu Lys Lys Glu Ile Tyr Ser Lys Val Gln Glu Asn 
385                 390                 395                 400 


Pro Glu Ala Glu Tyr Leu Leu Glu Glu Leu Glu Lys Gly Thr Phe Leu 
                405                 410                 415     


Pro Arg Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Leu Gln 
            420                 425                 430         


Phe Leu Glu Leu Gln Lys Ile Leu Lys Asn Ala Val Glu Tyr Leu Pro 
        435                 440                 445             


Phe Leu Lys Glu Glu Ser Glu Lys Ile Gln Gln Ile Met Thr Phe Arg 
    450                 455                 460                 


Ile Pro Tyr Tyr Val Gly Pro Leu Asn Gly Lys Lys Glu Gly Gln Asn 
465                 470                 475                 480 


Thr Phe Ala Trp Ala Val Arg Lys Asn Asn Glu Pro Val Tyr Pro Trp 
                485                 490                 495     


Asn Phe Glu Glu Val Ile Asp Thr Glu Ala Ser Ala Glu Gln Phe Ile 
            500                 505                 510         


Arg Arg Met Thr Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu 
        515                 520                 525             


Pro Lys Glu Ser Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu 
    530                 535                 540                 


Asn Asn Leu Arg Ile Asn Gly Thr Lys Ile Ser Val Glu Leu Lys Gln 
545                 550                 555                 560 


Glu Leu Tyr Glu Lys Leu Phe Gln Arg Tyr Arg Lys Val Ser Ala Lys 
                565                 570                 575     


Lys Leu Arg Asp Tyr Leu Val Arg Asn Gly Ile Thr Glu Lys Asp Ala 
            580                 585                 590         


Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Gly Thr Leu Arg Ala Tyr 
        595                 600                 605             


His Asp Phe Lys Glu Lys Leu Thr Gly Val Glu Leu Thr Glu Ala Glu 
    610                 615                 620                 


Lys Glu Thr Ile Ile Leu Asn Ile Thr Leu Phe Gly Glu Asp Lys Val 
625                 630                 635                 640 


Leu Leu Lys Lys Arg Met Gln Arg Leu Leu Pro Asn Leu Thr Glu Gly 
                645                 650                 655     


Gln Lys Lys Ala Ile Val Thr Leu Asn Tyr Ser Gly Trp Gly Arg Leu 
            660                 665                 670         


Ser Arg Glu Phe Leu Glu Glu Val Gln Ala Pro Asn Pro Glu Ser Gly 
        675                 680                 685             


Glu Ile Leu Ser Ile Ile Arg Ala Leu Trp Glu Thr Asn Ala Asn Leu 
    690                 695                 700                 


Met Gln Leu Leu Ser Asn Gln Tyr Gly Tyr Gln Glu Ile Leu Glu Gln 
705                 710                 715                 720 


Met Arg Ala Asp Trp Thr Gly His Lys Leu Asp Tyr Gln Thr Val Glu 
                725                 730                 735     


Glu Leu Tyr Val Ser Pro Ala Val Lys Arg Gln Ile Trp Gln Thr Leu 
            740                 745                 750         


Gln Val Val Lys Glu Leu Lys Lys Val Met Gly Gly Glu Pro Lys Arg 
        755                 760                 765             


Val Phe Leu Glu Val Ala Arg Glu Lys Met Glu Ser Gly Arg Thr Val 
    770                 775                 780                 


Ser Arg Lys Lys His Leu Lys Asp Leu Tyr Leu Ser Cys Lys Ser Glu 
785                 790                 795                 800 


Glu Arg Asn Trp Leu Ala Glu Leu Asp Arg His Glu Glu His Ser Tyr 
                805                 810                 815     


Arg Ser Asp Arg Leu Phe Leu Tyr Tyr Thr Gln Lys Gly Arg Cys Met 
            820                 825                 830         


Tyr Cys Gly Lys Pro Ile Asp Leu Gly His Leu Trp Asp Val Asn Met 
        835                 840                 845             


Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met Asp Asp Ser 
    850                 855                 860                 


Ile Arg Asn Arg Val Leu Thr Cys Arg Thr Cys Asn Ala Glu Lys Thr 
865                 870                 875                 880 


Asp His Tyr Pro Leu Lys Ala Glu Ile Arg Gln Lys Met Arg Pro Phe 
                885                 890                 895     


Trp Asp Ser Leu Lys Ala Gly Asn Phe Ile Glu Glu Glu Lys Trp Lys 
            900                 905                 910         


Arg Leu Ile Arg Ser Glu Glu Phe Thr Ser Thr Glu Leu Ala Gly Phe 
        915                 920                 925             


Ile Glu Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala Val Ala 
    930                 935                 940                 


Asp Ile Leu Lys Gln Ala Met Pro Glu Thr Glu Ile Val Tyr Val Lys 
945                 950                 955                 960 


Ala Lys Asn Val Ser Ala Phe Arg Gln Asp Phe Asn Phe Val Lys Asn 
                965                 970                 975     


Arg Asp Val Asn Asp Phe His His Ala Lys Asp Ala Tyr Leu Asn Ile 
            980                 985                 990         


Val Val Gly Asn Thr Tyr Phe Val  Lys Phe Thr Lys Asn  Ala Ser Trp 
        995                 1000                 1005             


Phe Ile  Glu Arg Asn Pro Gly  Arg Ser Tyr Asn Leu  Lys Arg Met 
    1010                 1015                 1020             


Phe Thr  Ser Asp Tyr Asp Val  Met Ser Asn Gly Glu  Ile Ala Trp 
    1025                 1030                 1035             


Lys Ala  Gly Asn Ser Gly Thr  Ile Thr Thr Val Arg  Lys Met Met 
    1040                 1045                 1050             


Lys Lys  Asn Ser Val Leu Phe  Thr Arg Arg Ser Phe  Glu Val Ser 
    1055                 1060                 1065             


Gly Gly  Leu Phe Asp Gln Gln  Leu Met Lys Lys Gly  Lys Gly Gln 
    1070                 1075                 1080             


Val Tyr  Val Lys Ser Gly Asp  Glu Arg Leu Arg Ser  Ile Glu Lys 
    1085                 1090                 1095             


Tyr Gly  Gly Tyr Asn Lys Ala  Thr Gly Ala Tyr Phe  Met Leu Val 
    1100                 1105                 1110             


Glu Ser  Glu Gly Lys Lys Gly  Val Lys Lys Arg Thr  Ile Glu Phe 
    1115                 1120                 1125             


Val Pro  Leu Tyr Arg Lys Ala  Glu Leu Glu Ala Ser  Glu Glu Lys 
    1130                 1135                 1140             


Met Leu  Glu Tyr Leu Arg Thr  Glu Ile Gly Leu Lys  Glu Pro His 
    1145                 1150                 1155             


Val Leu  Leu His Lys Ile Lys  Thr Asp Thr Leu Phe  Gln Ala Asp 
    1160                 1165                 1170             


Gly Phe  Lys Met His Leu Ser  Gly Arg Thr Gly Lys  Gln Leu Val 
    1175                 1180                 1185             


Phe Lys  Gly Ala Asn Gln Phe  Leu Val Ala Glu Asp  Tyr Glu Arg 
    1190                 1195                 1200             


Leu Leu  Lys Lys Val Gly Lys  Val Thr Glu Gln Leu  Lys Thr Asn 
    1205                 1210                 1215             


Lys Asn  Tyr Lys Ile Asn Asp  Tyr Asp Gly Ile Thr  Glu Glu Gly 
    1220                 1225                 1230             


Leu Lys  Ala Leu Tyr Ser Glu  Phe Leu Ser Lys Leu  Gln Lys Ala 
    1235                 1240                 1245             


Pro Tyr  Gly Asn Arg Leu Gly  Thr Gln Ala Glu Thr  Leu Glu Asn 
    1250                 1255                 1260             


Gly Lys  Glu Lys Phe Asp Lys  Leu Ser Leu Glu Asn  Lys Cys Gln 
    1265                 1270                 1275             


Val Leu  Ser Glu Ile Leu His  Leu Phe Gln Cys Asn  Ser Ser Ala 
    1280                 1285                 1290             


Ala Asp  Leu Ser Lys Leu Gly  Gly Pro Gly His Gly  Gly Ile Leu 
    1295                 1300                 1305             


Val Leu  Ser Asn Glu Ile Thr  Lys Cys Asn Lys Ile  Ser Ile Ile 
    1310                 1315                 1320             


His Gln  Ser Pro Ala Gly Ile  Tyr Glu Gln Glu Ile  Asp Leu Lys 
    1325                 1330                 1335             


Thr Leu  
    1340 


<210>  872
<211>  1340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  872

Met Glu Gln Glu Tyr Tyr Leu Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Ser Glu Tyr His Val Leu Arg Lys His Gly 
            20                  25                  30          


Lys Ala Leu Trp Gly Val Arg Leu Phe Glu Ser Ala Ser Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Met Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Asn 
    50                  55                  60                  


Trp Arg Ile Glu Ile Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys 
65                  70                  75                  80  


Lys Asp Pro Gly Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro 
                85                  90                  95      


Glu Asp Lys Arg Asp Ile Asn Gly Asn Cys Pro Glu Leu Pro Tyr Ala 
            100                 105                 110         


Leu Phe Val Asp Asp Asp Phe Thr Asp Lys Asp Tyr His Lys Lys Phe 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Asn Thr Glu Glu Thr 
    130                 135                 140                 


Pro Asp Ile Arg Leu Val Tyr Leu Ala Ile His His Met Met Lys His 
145                 150                 155                 160 


Arg Gly His Phe Leu Leu Ser Gly Asp Ile Asn Glu Ile Lys Glu Phe 
                165                 170                 175     


Gly Thr Thr Phe Ser Lys Leu Leu Glu Asn Ile Lys Asn Glu Glu Leu 
            180                 185                 190         


Asp Trp Asn Leu Glu Leu Gly Lys Glu Glu Tyr Ala Val Val Glu Ser 
        195                 200                 205             


Ile Leu Lys Asp Asn Met Leu Asn Arg Ser Thr Lys Lys Thr Arg Leu 
    210                 215                 220                 


Ile Lys Ala Leu Lys Ala Lys Ser Ile Cys Glu Lys Ala Val Leu Asn 
225                 230                 235                 240 


Leu Leu Ala Gly Gly Thr Val Lys Leu Ser Asp Ile Phe Gly Leu Glu 
                245                 250                 255     


Glu Leu Asn Glu Thr Glu Arg Pro Lys Ile Ser Phe Ala Asp Asn Gly 
            260                 265                 270         


Tyr Asp Asp Tyr Ile Gly Glu Val Glu Asn Glu Leu Gly Glu Gln Phe 
        275                 280                 285             


Tyr Ile Ile Glu Thr Ala Lys Ala Val Tyr Asp Trp Ala Val Leu Val 
    290                 295                 300                 


Glu Ile Leu Gly Lys Tyr Thr Ser Ile Ser Glu Ala Lys Val Ala Thr 
305                 310                 315                 320 


Tyr Glu Lys His Lys Ser Asp Leu Gln Phe Leu Lys Lys Ile Val Arg 
                325                 330                 335     


Lys Tyr Leu Thr Lys Glu Glu Tyr Lys Asp Ile Phe Val Ser Thr Ser 
            340                 345                 350         


Asp Lys Leu Lys Asn Tyr Ser Ala Tyr Ile Gly Met Thr Lys Ile Asn 
        355                 360                 365             


Gly Lys Lys Val Asp Leu Gln Ser Lys Arg Cys Ser Lys Glu Glu Phe 
    370                 375                 380                 


Tyr Asp Phe Ile Lys Lys Asn Val Leu Lys Lys Leu Glu Gly Gln Pro 
385                 390                 395                 400 


Glu Tyr Glu Tyr Leu Lys Glu Glu Leu Glu Arg Glu Thr Phe Leu Pro 
                405                 410                 415     


Lys Gln Val Asn Arg Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
            420                 425                 430         


Tyr Glu Leu Lys Lys Ile Leu Gly Asn Leu Arg Asp Lys Ile Asp Leu 
        435                 440                 445             


Ile Lys Glu Asn Glu Asp Lys Leu Val Gln Leu Phe Glu Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Asn Lys Ile Asp Asp Gly Lys Glu Gly 
465                 470                 475                 480 


Lys Phe Thr Trp Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp 
                485                 490                 495     


Asn Phe Glu Asn Val Val Asp Ile Glu Ala Ser Ala Glu Lys Phe Ile 
            500                 505                 510         


Arg Arg Met Thr Asn Lys Cys Thr Tyr Leu Met Gly Glu Asp Val Leu 
        515                 520                 525             


Pro Lys Asp Ser Leu Leu Tyr Ser Lys Tyr Met Val Leu Asn Glu Leu 
    530                 535                 540                 


Asn Asn Val Lys Leu Asp Gly Glu Lys Leu Ser Val Glu Leu Lys Gln 
545                 550                 555                 560 


Arg Leu Tyr Thr Asp Val Phe Cys Lys Tyr Arg Lys Val Thr Val Lys 
                565                 570                 575     


Lys Ile Lys Asn Tyr Leu Lys Cys Glu Gly Ile Ile Ser Gly Asn Val 
            580                 585                 590         


Glu Ile Thr Gly Ile Asp Gly Asp Phe Lys Ala Ser Leu Thr Ala Tyr 
        595                 600                 605             


His Asp Phe Lys Glu Ile Leu Thr Gly Thr Glu Leu Ala Lys Lys Asp 
    610                 615                 620                 


Lys Glu Asn Ile Ile Thr Asn Ile Val Leu Phe Gly Asp Asp Lys Lys 
625                 630                 635                 640 


Leu Leu Lys Lys Arg Leu Asn Arg Leu Tyr Pro Gln Ile Thr Pro Asn 
                645                 650                 655     


Gln Leu Lys Lys Ile Cys Ala Leu Ser Tyr Thr Gly Trp Gly Arg Phe 
            660                 665                 670         


Ser Lys Lys Phe Leu Glu Glu Ile Thr Ala Pro Asp Pro Glu Thr Gly 
        675                 680                 685             


Glu Val Trp Asn Ile Ile Thr Ala Leu Trp Glu Ser Asn Asn Asn Leu 
    690                 695                 700                 


Met Gln Leu Leu Ser Asn Glu Tyr Arg Phe Met Glu Glu Val Glu Thr 
705                 710                 715                 720 


Tyr Asn Met Gly Lys Gln Thr Lys Thr Leu Ser Tyr Glu Thr Val Glu 
                725                 730                 735     


Asn Met Tyr Val Ser Pro Ser Val Lys Arg Gln Ile Trp Gln Thr Leu 
            740                 745                 750         


Lys Ile Val Lys Glu Leu Glu Lys Val Met Lys Glu Ser Pro Lys Arg 
        755                 760                 765             


Val Phe Ile Glu Met Ala Arg Glu Lys Gln Glu Ser Lys Arg Thr Glu 
    770                 775                 780                 


Ser Arg Lys Lys Gln Leu Ile Asp Leu Tyr Lys Ala Cys Lys Asn Glu 
785                 790                 795                 800 


Glu Lys Asp Trp Val Lys Glu Leu Gly Asp Gln Glu Glu Gln Lys Leu 
                805                 810                 815     


Arg Ser Asp Lys Leu Tyr Leu Tyr Tyr Thr Gln Lys Gly Arg Cys Met 
            820                 825                 830         


Tyr Ser Gly Glu Val Ile Glu Leu Lys Asp Leu Trp Asp Asn Thr Lys 
        835                 840                 845             


Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met Asp Asp Ser 
    850                 855                 860                 


Leu Asn Asn Arg Val Leu Val Lys Lys Lys Tyr Asn Ala Thr Lys Ser 
865                 870                 875                 880 


Asp Lys Tyr Pro Leu Asn Glu Asn Ile Arg His Glu Arg Lys Gly Phe 
                885                 890                 895     


Trp Lys Ser Leu Leu Asp Gly Gly Phe Ile Ser Lys Glu Lys Tyr Glu 
            900                 905                 910         


Arg Leu Ile Arg Asn Thr Glu Leu Ser Pro Glu Glu Leu Ala Gly Phe 
        915                 920                 925             


Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ser Thr Lys Ala Val Ala 
    930                 935                 940                 


Glu Ile Leu Lys Gln Val Phe Pro Glu Ser Glu Ile Val Tyr Val Lys 
945                 950                 955                 960 


Ala Gly Thr Val Ser Arg Phe Arg Lys Asp Phe Glu Leu Leu Lys Val 
                965                 970                 975     


Arg Glu Val Asn Asp Leu His His Ala Lys Asp Ala Tyr Leu Asn Ile 
            980                 985                 990         


Val Val Gly Asn Ser Tyr Tyr Val  Lys Phe Thr Lys Asn  Ala Ser Trp 
        995                 1000                 1005             


Phe Ile  Lys Glu Asn Pro Gly  Arg Thr Tyr Asn Leu  Lys Lys Met 
    1010                 1015                 1020             


Phe Thr  Ser Gly Trp Asn Ile  Glu Arg Asn Gly Glu  Val Ala Trp 
    1025                 1030                 1035             


Glu Val  Gly Lys Lys Gly Thr  Ile Val Thr Val Lys  Gln Ile Met 
    1040                 1045                 1050             


Asn Lys  Asn Asn Ile Leu Val  Thr Arg Gln Val His  Glu Ala Lys 
    1055                 1060                 1065             


Gly Gly  Leu Phe Asp Gln Gln  Ile Met Lys Lys Gly  Lys Gly Gln 
    1070                 1075                 1080             


Ile Ala  Ile Lys Glu Thr Asp  Glu Arg Leu Ala Ser  Ile Glu Lys 
    1085                 1090                 1095             


Tyr Gly  Gly Tyr Asn Lys Ala  Ala Gly Ala Tyr Phe  Met Leu Val 
    1100                 1105                 1110             


Glu Ser  Lys Asp Lys Lys Gly  Lys Thr Ile Arg Thr  Ile Glu Phe 
    1115                 1120                 1125             


Ile Pro  Leu Tyr Leu Lys Asn  Lys Ile Glu Ser Asp  Glu Ser Ile 
    1130                 1135                 1140             


Ala Leu  Asn Phe Leu Glu Lys  Gly Arg Gly Leu Lys  Glu Pro Lys 
    1145                 1150                 1155             


Ile Leu  Leu Lys Lys Ile Lys  Ile Asp Thr Leu Phe  Asp Val Asp 
    1160                 1165                 1170             


Gly Phe  Lys Met Trp Leu Ser  Gly Arg Thr Gly Asp  Arg Leu Leu 
    1175                 1180                 1185             


Phe Lys  Cys Ala Asn Gln Leu  Ile Leu Asp Glu Lys  Ile Ile Val 
    1190                 1195                 1200             


Thr Met  Lys Lys Ile Val Lys  Phe Ile Gln Arg Arg  Gln Glu Asn 
    1205                 1210                 1215             


Arg Glu  Leu Lys Leu Ser Asp  Lys Asp Gly Ile Asp  Asn Glu Val 
    1220                 1225                 1230             


Leu Met  Glu Ile Tyr Asn Thr  Phe Val Asp Lys Leu  Glu Asn Thr 
    1235                 1240                 1245             


Val Tyr  Arg Ile Arg Leu Ser  Glu Gln Ala Lys Thr  Leu Ile Asp 
    1250                 1255                 1260             


Lys Gln  Lys Glu Phe Glu Arg  Leu Ser Leu Glu Asp  Lys Ser Ser 
    1265                 1270                 1275             


Thr Leu  Phe Glu Ile Leu His  Ile Phe Gln Cys Gln  Ser Ser Ala 
    1280                 1285                 1290             


Ala Asn  Leu Lys Met Ile Gly  Gly Pro Gly Lys Ala  Gly Ile Leu 
    1295                 1300                 1305             


Val Met  Asn Asn Asn Ile Ser  Lys Cys Asn Lys Ile  Ser Ile Ile 
    1310                 1315                 1320             


Asn Gln  Ser Pro Thr Gly Ile  Phe Glu Asn Glu Ile  Asp Leu Leu 
    1325                 1330                 1335             


Lys Ile  
    1340 


<210>  873
<211>  1340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  873

Met Gly Glu Glu Tyr Phe Leu Gly Leu Asp Met Gly Thr Gly Ser Leu 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Asp Lys Tyr Gln Ile Leu Arg Arg His Gly 
            20                  25                  30          


Asn Ala Leu Trp Gly Val Arg Leu Phe Glu Thr Ala Asn Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Met Phe Arg Thr Asn Arg Arg Arg Leu Asp Arg Gln Gly 
    50                  55                  60                  


Trp Arg Ile Lys Ile Leu Gln Glu Leu Phe Ala Glu Glu Ile Cys Arg 
65                  70                  75                  80  


Val Asp Pro Gly Phe Tyr Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro 
                85                  90                  95      


Glu Asp Lys Arg Asp Ala Asn Gly Glu Cys Pro Ala Leu Pro Tyr Ala 
            100                 105                 110         


Leu Phe Ala Asp Ala Gln Tyr Thr Asp Lys Asp Tyr His Arg Glu Phe 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu Thr Glu Thr Thr 
    130                 135                 140                 


Pro Asp Ile Arg Leu Val Tyr Leu Ala Phe His His Met Met Lys His 
145                 150                 155                 160 


Arg Gly His Phe Leu Leu Ser Gly Asp Ile Ser Gln Val Thr Glu Phe 
                165                 170                 175     


Arg Asn Thr Phe Leu Gln Met Ile Ala Asn Ile Lys Ser Glu Glu Leu 
            180                 185                 190         


Asp Phe Ser Leu Glu Ile Glu Glu Thr Gln Met Gly Gln Ile Glu Ala 
        195                 200                 205             


Val Leu Lys Asp His Lys Leu Thr Arg Ser Glu Lys Lys Arg Arg Leu 
    210                 215                 220                 


Thr Gln Ile Leu Lys Pro Ala Thr Ala Cys Glu Lys Ala Ile Phe Thr 
225                 230                 235                 240 


Leu Leu Ser Gly Gly Thr Val Lys Leu Ser Asp Ile Phe Gly Lys Ala 
                245                 250                 255     


Glu Leu Asp Glu Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Asn Gly 
            260                 265                 270         


Tyr Asp Asp Tyr Ser Ala Ser Val Glu Ala Asp Leu Gly Glu Gln Tyr 
        275                 280                 285             


Tyr Ile Ile Glu Ser Ala Lys Ala Val Tyr Asp Trp Ser Val Leu Ser 
    290                 295                 300                 


Asp Ile Leu Gly Asp Ser Val Ser Ile Ser Asp Ala Lys Val Lys Leu 
305                 310                 315                 320 


Tyr Glu Lys His Lys Lys Asp Leu Ala Tyr Leu Lys Lys Val Val Arg 
                325                 330                 335     


Glu Asn Leu Pro Arg Glu Val Tyr Asn Gln Val Phe Val Asn Thr Asp 
            340                 345                 350         


Glu Lys Leu Cys Asn Tyr Cys Ala Tyr Ile Gly Met Ala Lys Lys Asn 
        355                 360                 365             


Gly Arg Lys Val Ala Leu Glu Gly Lys Arg Cys Thr Arg Glu Glu Phe 
    370                 375                 380                 


Glu Ser Phe Leu Arg Lys Lys Ile Val Asn Lys Ile Glu Asp Lys Glu 
385                 390                 395                 400 


Ile Gln Glu Tyr Leu Ser Glu Glu Ile Glu Lys Gly Thr Phe Leu Pro 
                405                 410                 415     


Arg Gln Val Thr Lys Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
            420                 425                 430         


Tyr Glu Leu Arg Lys Ile Ile Asp Asn Leu Ser Pro Arg Met Pro Phe 
        435                 440                 445             


Ile Arg Asp Asn Gly Asp Lys Ile Ile Gln Leu Phe Thr Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Gly Lys Gly Glu Gly Ser Glu Lys Phe 
465                 470                 475                 480 


Ser Trp Ala Val Arg Arg Thr Asp Glu Ala Ile Tyr Pro Trp Asn Phe 
                485                 490                 495     


Lys Asp Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile Cys Arg 
            500                 505                 510         


Met Thr Asn Lys Cys Thr Tyr Leu Tyr Gly Glu Asp Val Leu Pro Lys 
        515                 520                 525             


Asp Ser Pro Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn 
    530                 535                 540                 


Leu Lys Leu Asp Gly Gln Ser Val Ser Val Glu Leu Lys Gln Arg Ile 
545                 550                 555                 560 


Tyr Gln Glu Leu Phe Cys His Thr Arg Lys Val Thr Gln Lys Lys Leu 
                565                 570                 575     


Val Ser Tyr Leu Val Arg Glu Gly Ile Thr Gly Lys Asn Val Glu Ile 
            580                 585                 590         


Ser Gly Ile Asp Gly Asp Phe Lys Ala Ser Leu Thr Ala Tyr His Asp 
        595                 600                 605             


Phe Lys Glu Lys Leu Thr Gly Val Glu Leu Ser Gln Gln Glu Gln Glu 
    610                 615                 620                 


Glu Ile Val Arg Asn Ile Val Leu Phe Gly Glu Asp Lys Lys Leu Leu 
625                 630                 635                 640 


Lys Gln Arg Leu Glu Gly Arg Phe Pro Gln Leu Thr Gln Lys Gln Ile 
                645                 650                 655     


Ser Val Ile Ala Thr Leu Ser Tyr Lys Gly Trp Gly Arg Leu Ser Arg 
            660                 665                 670         


Glu Phe Leu Glu Asn Ile Thr Val Pro Ala Pro Gly Thr Gly Glu Val 
        675                 680                 685             


Trp Asn Ile Ile Thr Ala Leu Trp Glu Thr Asn Asp Asn Leu Met Gln 
    690                 695                 700                 


Leu Leu Tyr Gly Glu Tyr Gly Phe Gln Thr Glu Ile Glu Cys Phe Asn 
705                 710                 715                 720 


Ala Lys Glu Lys Lys Lys Ser Leu Ser Tyr Glu Thr Ile Glu Glu Ser 
                725                 730                 735     


Tyr Val Ser Pro Ser Val Lys Arg Gln Ile Trp Gln Thr Leu Gln Val 
            740                 745                 750         


Val Lys Glu Leu Lys Lys Val Met Arg Gly Asp Pro Lys Arg Val Phe 
        755                 760                 765             


Ile Glu Met Ala Arg Glu Asn Gln Glu Ser Lys Arg Thr Glu Ser Arg 
    770                 775                 780                 


Lys Lys Thr Leu Leu Asp Leu Tyr Lys Asn Cys Lys Lys Glu Glu Pro 
785                 790                 795                 800 


Glu Trp Ile Ala Glu Leu Thr Glu His Leu Glu Lys Leu Asp Asp Ser 
                805                 810                 815     


Gln Leu Arg Lys Asp Lys Leu Phe Leu Tyr Tyr Thr Gln Lys Gly Arg 
            820                 825                 830         


Cys Met Tyr Ser Gly Glu Arg Ile Glu Leu Glu Asp Leu Trp Asp Asn 
        835                 840                 845             


Thr Arg Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met Asp 
    850                 855                 860                 


Asp Ser Val Asp Asn Arg Val Leu Val Lys Lys Glu Tyr Asn Ala Lys 
865                 870                 875                 880 


Lys Thr Asp Val Tyr Pro Ile Ala Ala Asp Ile Arg Gly Lys Met Ser 
                885                 890                 895     


Pro Phe Trp Lys Lys Leu Leu Asp Asn Lys Leu Ile Ser Lys Lys Lys 
            900                 905                 910         


Tyr Asp Arg Leu Val Arg Thr Glu Glu Leu Thr Pro Glu Glu Leu Ala 
        915                 920                 925             


Gly Phe Ile Asp Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Val 
    930                 935                 940                 


Val Ala Glu Ile Leu Lys Glu Ala Phe Pro Asp Thr Glu Ile Val Tyr 
945                 950                 955                 960 


Val Lys Ala Gly Asn Val Ser Asn Phe Arg His Met Phe Asp Ile Val 
                965                 970                 975     


Lys Val Arg Glu Leu Asn Asp Leu His His Ala Lys Asp Ala Tyr Leu 
            980                 985                 990         


Asn Ile Val Val Gly Asn Ala Tyr  Tyr Val Lys Phe Asn  Lys Asn Ala 
        995                 1000                 1005             


Ala Trp  Phe Ile Lys Glu Asn  Pro Gly Arg Ser Tyr  Asn Leu Lys 
    1010                 1015                 1020             


Arg Met  Phe Asp Tyr Asp Ile  Arg Arg Asn Asn Glu  Val Ala Trp 
    1025                 1030                 1035             


Leu Ala  Gly Ser Thr Gly Thr  Ile Ser Gln Val Lys  Asn Met Val 
    1040                 1045                 1050             


Ser Lys  Asn His Ile Leu Val  Thr Arg Lys Ser Tyr  Glu Val Thr 
    1055                 1060                 1065             


Gly Gly  Leu Phe Asp Gln Gln  Leu Met Lys Lys Gly  Lys Gly Gln 
    1070                 1075                 1080             


Val Pro  Val Lys Thr Ser Asp  Glu Arg Leu Gly Asn  Ile Glu Lys 
    1085                 1090                 1095             


Tyr Gly  Gly Tyr Asn Lys Ala  Ser Gly Ala Tyr Phe  Met Leu Val 
    1100                 1105                 1110             


Lys Ser  Lys Asn Lys Lys Gly  Glu Glu Gln Arg Thr  Ile Glu Tyr 
    1115                 1120                 1125             


Val Pro  Ile Tyr Leu Lys His  Tyr Ile Glu Glu Ser  Lys Glu Asn 
    1130                 1135                 1140             


Ala Leu  Asp Tyr Leu Lys Asn  Glu Arg Lys Leu Asn  Glu Pro Glu 
    1145                 1150                 1155             


Ile Leu  Leu Asp Lys Ile Lys  Lys Asp Thr Leu Phe  Arg Val Asn 
    1160                 1165                 1170             


Gly Phe  Tyr Met Cys Leu Ser  Lys Arg Thr Gly Lys  Gln Leu Ala 
    1175                 1180                 1185             


Phe Lys  Gly Ala Asn Glu Leu  Leu Leu Pro Glu Pro  Glu Thr Leu 
    1190                 1195                 1200             


Leu Leu  Lys Lys Ile Thr Lys  Tyr Ile Gln Arg Arg  Lys Glu Asn 
    1205                 1210                 1215             


Lys Asp  Leu Lys Leu Thr Lys  Gln Asp Asn Ile Thr  Glu Lys Glu 
    1220                 1225                 1230             


Leu Leu  Glu Leu Tyr Asp Val  Phe Thr Glu Lys Leu  Asn His Ser 
    1235                 1240                 1245             


Val Tyr  Ala Val Cys Leu Gly  Thr Gln Gly Lys Thr  Leu Val Gln 
    1250                 1255                 1260             


Lys Lys  Pro Ala Phe Val Ala  Leu Pro Val Glu Glu  Lys Cys Ile 
    1265                 1270                 1275             


Val Leu  Ser Glu Ile Leu His  Leu Phe Gln Cys Gln  Ser Gly Ser 
    1280                 1285                 1290             


Ala Asn  Leu Lys Leu Ile Gly  Gly Pro Ala Ser Ala  Gly Ile Leu 
    1295                 1300                 1305             


Tyr Leu  Asn Ser Asn Ile Ser  Asp Ala Gly Glu Ile  Leu Ile Ile 
    1310                 1315                 1320             


Asn Gln  Ser Pro Ser Gly Ile  Phe Glu Lys Arg Ile  Asp Leu Leu 
    1325                 1330                 1335             


Arg Leu  
    1340 


<210>  874
<211>  1340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  874

Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Val Leu Thr Asp Asp Tyr Arg Leu Val Ser Lys Lys Met 
            20                  25                  30          


Lys Val Ala Gly Asn Thr Glu Lys Ser Ser Thr Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Leu Ala Arg Arg Arg Gln Arg Ile Leu 
65                  70                  75                  80  


Glu Leu Gln Lys Ile Phe Ala Pro Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      


Phe Phe Ala Arg Leu Asn Glu Ser Phe Leu Val Pro Asp Glu Lys Lys 
            100                 105                 110         


Gln Ser Arg His Pro Val Phe Ala Thr Ile Lys Gln Glu Lys Ser Tyr 
        115                 120                 125             


His Gln Thr Tyr Pro Thr Ile Tyr His Leu Arg Gln Ala Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Met Ala His 
145                 150                 155                 160 


Leu Leu Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     


Glu Asn Ser Ser Val Thr Glu Thr Phe Arg Gln Phe Leu Ser Thr Tyr 
            180                 185                 190         


Asn Gln Gln Phe Ser Glu Ala Gly Asp Lys Gln Thr Glu Lys Leu Asp 
        195                 200                 205             


Glu Ala Val Asp Cys Ser Phe Val Phe Thr Glu Lys Met Ser Lys Thr 
    210                 215                 220                 


Lys Lys Ala Glu Thr Leu Leu Lys Tyr Phe Pro His Glu Lys Ser Asn 
225                 230                 235                 240 


Gly Tyr Leu Ser Gln Phe Ile Lys Leu Met Val Gly Asn Gln Gly Asn 
                245                 250                 255     


Phe Lys Asn Val Phe Gly Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser 
            260                 265                 270         


Lys Glu Thr Tyr Glu Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly 
        275                 280                 285             


Asp Asp Tyr Ile Asp Leu Phe Val Gln Ala Lys Asn Val Tyr Asp Ala 
    290                 295                 300                 


Val Leu Leu Ser Glu Ile Leu Ser Asp Ser Thr Lys Asn Thr Arg Ala 
305                 310                 315                 320 


Lys Leu Ser Ala Gly Met Ile Arg Arg Tyr Asp Ala His Lys Glu Asp 
                325                 330                 335     


Leu Val Leu Leu Lys Arg Phe Val Lys Glu Asn Leu Pro Lys Lys Tyr 
            340                 345                 350         


Arg Ala Phe Phe Gly Asp Asn Ser Val Asn Gly Tyr Ala Gly Tyr Ile 
        355                 360                 365             


Glu Gly His Ala Thr Gln Glu Ala Phe Tyr Lys Phe Val Lys Lys Glu 
    370                 375                 380                 


Leu Thr Gly Ile Arg Gly Ser Glu Val Phe Leu Thr Lys Ile Glu Gln 
385                 390                 395                 400 


Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro 
                405                 410                 415     


His Gln Ile His Leu Ser Glu Leu Arg Ala Ile Ile Ala Asn Gln Lys 
            420                 425                 430         


Lys His Tyr Pro Phe Leu Lys Glu Glu Gln Glu Lys Leu Glu Ser Leu 
        435                 440                 445             


Leu Thr Phe Lys Ile Pro Tyr Tyr Val Gly Pro Leu Ala Lys Lys Gln 
    450                 455                 460                 


Glu Asn Ser Pro Phe Ala Trp Leu Ile Arg Lys Ser Glu Glu Lys Ile 
465                 470                 475                 480 


Lys Pro Trp Asn Leu Pro Glu Ile Val Asp Met Glu Gly Ser Ala Val 
                485                 490                 495     


Arg Phe Ile Glu Arg Met Ile Asn Thr Asp Met Tyr Met Pro His Asn 
            500                 505                 510         


Lys Val Leu Pro Lys Asn Ser Leu Leu Tyr Gln Lys Phe Ser Ile Tyr 
        515                 520                 525             


Asn Glu Leu Thr Lys Val Arg Tyr Gln Asp Glu Arg Gly Gln Met Asn 
    530                 535                 540                 


Tyr Phe Ser Ser Ile Glu Lys Lys Glu Ile Phe His Glu Leu Phe Glu 
545                 550                 555                 560 


Lys Asn Arg Lys Val Thr Lys Lys Asp Leu Gln Glu Phe Leu Tyr Leu 
                565                 570                 575     


Lys Tyr Asp Ile Lys His Ala Glu Leu Ser Gly Ile Glu Lys Ala Phe 
            580                 585                 590         


Asn Ala Ser Tyr Thr Thr Tyr His Asp Phe Leu Thr Met Ser Glu Asn 
        595                 600                 605             


Lys Arg Glu Met Lys Gln Trp Leu Glu Asp Pro Glu Leu Ala Ser Met 
    610                 615                 620                 


Phe Glu Glu Ile Ile Lys Thr Leu Thr Val Phe Glu Asp Arg Glu Met 
625                 630                 635                 640 


Ile Lys Thr Arg Leu Ser His His Glu Ala Thr Leu Gly Lys His Ile 
                645                 650                 655     


Ile Lys Lys Leu Thr Lys Lys His Tyr Thr Gly Trp Gly Arg Leu Ser 
            660                 665                 670         


Lys Glu Leu Ile Gln Gly Ile Arg Asp Lys Gln Ser Asn Lys Thr Ile 
        675                 680                 685             


Leu Asp Tyr Leu Ile Asn Asp Asp Asp Phe Pro His His Arg Asn Arg 
    690                 695                 700                 


Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys Lys Glu 
705                 710                 715                 720 


Ile Lys Lys Ala Gln Met Ile Thr Asp Thr Glu Asn Leu Glu Glu Ile 
                725                 730                 735     


Val Lys Glu Leu Thr Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln 
            740                 745                 750         


Ser Leu Lys Ile Val Asp Glu Ile Val Gly Ile Met Gly Tyr Glu Pro 
        755                 760                 765             


Ala Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr Gly Arg 
    770                 775                 780                 


Gly Leu Lys Ser Ser Arg Pro Arg Leu Lys Ala Leu Glu Glu Ser Leu 
785                 790                 795                 800 


Lys Asp Phe Gly Ser Gln Leu Leu Lys Glu Tyr Pro Thr Asp Asn Ser 
                805                 810                 815     


Ser Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg 
            820                 825                 830         


Asp Met Tyr Thr Gly Ala Pro Leu Asp Ile His Arg Leu Ser Asp Tyr 
        835                 840                 845             


Asp Ile Asp His Ile Ile Pro Arg Ser Phe Thr Thr Asp Asn Ser Ile 
    850                 855                 860                 


Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg Leu Lys Lys Asp 
865                 870                 875                 880 


Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg Ser Phe Trp Tyr 
                885                 890                 895     


Asp Leu Tyr Ser Ser Lys Leu Ile Ser Lys Arg Lys Leu Asp Asn Leu 
            900                 905                 910         


Thr Lys Ile Lys Leu Thr Glu Glu Asp Lys Ala Gly Phe Ile Lys Arg 
        915                 920                 925             


Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gly Ile Leu 
    930                 935                 940                 


His His Arg Phe Asn Lys Ala Glu Asp Thr Asn Asp Pro Ile Arg Lys 
945                 950                 955                 960 


Val Arg Ile Ile Thr Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Asn 
                965                 970                 975     


Arg Phe Gly Ile Tyr Lys Val Arg Glu Ile Asn Glu Tyr His His Ala 
            980                 985                 990         


His Asp Ala Tyr Leu Asn Gly Val  Val Ala Leu Ala Leu  Leu Lys Lys 
        995                 1000                 1005             


Tyr Pro  Gln Leu Ala Pro Glu  Phe Val Tyr Gly Glu  Tyr Leu Lys 
    1010                 1015                 1020             


Phe Asn  Ala His Lys Ala Asn  Lys Ala Thr Val Lys  Lys Glu Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Lys Phe  Phe Glu Ser Asp Thr  Pro Val Cys 
    1040                 1045                 1050             


Asp Glu  Asn Gly Glu Ile Phe  Trp Asp Lys Ser Lys  Ser Ile Ala 
    1055                 1060                 1065             


Gln Val  Lys Lys Val Ile Asn  His His His Met Asn  Ile Val Lys 
    1070                 1075                 1080             


Lys Thr  Glu Ile Gln Lys Gly  Gly Phe Ser Lys Glu  Thr Val Glu 
    1085                 1090                 1095             


Pro Lys  Lys Asp Ser Ser Lys  Leu Leu Pro Arg Lys  Asn Asn Trp 
    1100                 1105                 1110             


Asp Pro  Ala Lys Tyr Gly Gly  Leu Gly Ser Pro Asn  Val Ala Tyr 
    1115                 1120                 1125             


Thr Val  Ala Phe Thr Tyr Glu  Lys Gly Lys Ala Arg  Lys Arg Thr 
    1130                 1135                 1140             


Asn Ala  Leu Glu Gly Ile Thr  Ile Met Glu Arg Glu  Ala Phe Glu 
    1145                 1150                 1155             


Gln Ser  Pro Val Leu Phe Leu  Lys Asn Lys Gly Tyr  Glu Gln Ala 
    1160                 1165                 1170             


Glu Ile  Glu Met Lys Leu Pro  Lys Tyr Ala Leu Phe  Glu Leu Glu 
    1175                 1180                 1185             


Asn Gly  Arg Lys Arg Met Val  Ala Ser Asn Lys Glu  Ala Gln Lys 
    1190                 1195                 1200             


Ala Asn  Ser Phe Leu Leu Pro  Glu His Leu Val Thr  Leu Leu Tyr 
    1205                 1210                 1215             


His Ala  Lys Gln Tyr Asp Glu  Ile Ser His Lys Glu  Ser Phe Asp 
    1220                 1225                 1230             


Tyr Val  Asn Glu His His Lys  Glu Phe Ser Glu Val  Phe Ala Arg 
    1235                 1240                 1245             


Val Leu  Glu Phe Ala Gly Lys  Tyr Thr Leu Ala Glu  Lys Asn Ile 
    1250                 1255                 1260             


Glu Lys  Leu Glu Lys Ile Tyr  Lys Glu Asn Gln Thr  Asp Asp Leu 
    1265                 1270                 1275             


Ala Lys  Leu Ala Ser Ser Phe  Val Asn Leu Met Gln  Phe Asn Ala 
    1280                 1285                 1290             


Met Gly  Ala Pro Ala Asp Phe  Lys Phe Phe Asp Val  Thr Ile Pro 
    1295                 1300                 1305             


Arg Lys  Arg Tyr Thr Ser Leu  Thr Glu Ile Trp Gln  Ser Thr Ile 
    1310                 1315                 1320             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Arg Met 
    1325                 1330                 1335             


Gly Lys  
    1340 


<210>  875
<211>  1340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  875

Met Glu Gln Glu Tyr Tyr Leu Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Ser Glu Tyr His Val Leu Arg Lys His Gly 
            20                  25                  30          


Lys Ala Leu Trp Gly Val Arg Leu Phe Glu Ser Ala Ser Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Met Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Asn 
    50                  55                  60                  


Trp Arg Ile Glu Ile Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys 
65                  70                  75                  80  


Lys Asp Pro Gly Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro 
                85                  90                  95      


Glu Asp Lys Arg Asp Ile Asn Gly Asn Cys Pro Glu Leu Pro Tyr Ala 
            100                 105                 110         


Leu Phe Val Asp Asp Asp Phe Thr Asp Lys Asp Tyr His Lys Lys Phe 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Asn Thr Glu Glu Thr 
    130                 135                 140                 


Pro Asp Ile Arg Leu Val Tyr Leu Ala Ile His His Met Met Lys His 
145                 150                 155                 160 


Arg Gly His Phe Leu Leu Ser Gly Asp Ile Asn Glu Ile Lys Glu Phe 
                165                 170                 175     


Gly Thr Thr Phe Ser Lys Leu Leu Glu Asn Ile Lys Asn Glu Glu Leu 
            180                 185                 190         


Asp Trp Asn Leu Glu Leu Gly Lys Glu Glu Tyr Ala Val Val Glu Ser 
        195                 200                 205             


Ile Leu Lys Asp Asn Met Leu Asn Arg Ser Thr Lys Lys Thr Arg Leu 
    210                 215                 220                 


Ile Lys Ala Leu Lys Ala Lys Ser Ile Cys Glu Lys Ala Val Leu Asn 
225                 230                 235                 240 


Leu Leu Ala Gly Gly Thr Val Lys Leu Ser Asp Ile Phe Gly Leu Glu 
                245                 250                 255     


Glu Leu Asn Glu Thr Glu Arg Pro Lys Ile Ser Phe Ala Asp Asn Gly 
            260                 265                 270         


Tyr Asp Asp Tyr Ile Gly Glu Val Glu Asn Glu Leu Gly Glu Gln Phe 
        275                 280                 285             


Tyr Ile Ile Glu Thr Ala Lys Ala Val Tyr Asp Trp Ala Val Leu Val 
    290                 295                 300                 


Glu Ile Leu Gly Lys Tyr Thr Ser Ile Ser Glu Ala Lys Val Ala Thr 
305                 310                 315                 320 


Tyr Glu Lys His Lys Ser Asp Leu Gln Phe Leu Lys Lys Ile Val Arg 
                325                 330                 335     


Lys Tyr Leu Thr Lys Glu Glu Tyr Lys Asp Ile Phe Val Ser Thr Ser 
            340                 345                 350         


Asp Lys Leu Lys Asn Tyr Ser Ala Tyr Ile Gly Met Thr Lys Ile Asn 
        355                 360                 365             


Gly Lys Lys Val Asp Leu Gln Ser Lys Arg Cys Ser Lys Glu Glu Phe 
    370                 375                 380                 


Tyr Asp Phe Ile Lys Lys Asn Val Leu Lys Lys Leu Glu Gly Gln Pro 
385                 390                 395                 400 


Glu Tyr Glu Tyr Leu Lys Glu Glu Leu Glu Arg Glu Thr Phe Leu Pro 
                405                 410                 415     


Lys Gln Val Asn Arg Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
            420                 425                 430         


Tyr Glu Leu Lys Lys Ile Leu Gly Asn Leu Arg Asp Lys Ile Asp Leu 
        435                 440                 445             


Ile Lys Glu Asn Glu Asp Lys Leu Val Gln Leu Phe Glu Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Asn Lys Ile Asp Asp Gly Lys Glu Gly 
465                 470                 475                 480 


Lys Phe Thr Trp Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp 
                485                 490                 495     


Asn Phe Glu Asn Val Val Asp Ile Glu Ala Ser Ala Glu Lys Phe Ile 
            500                 505                 510         


Arg Arg Met Thr Asn Lys Cys Thr Tyr Leu Met Gly Glu Asp Val Leu 
        515                 520                 525             


Pro Lys Asp Ser Leu Leu Tyr Ser Lys Tyr Met Val Leu Asn Glu Leu 
    530                 535                 540                 


Asn Asn Val Lys Leu Asp Gly Glu Lys Leu Ser Val Glu Leu Lys Gln 
545                 550                 555                 560 


Arg Leu Tyr Thr Asp Val Phe Cys Lys Tyr Arg Lys Val Thr Val Lys 
                565                 570                 575     


Lys Ile Lys Asn Tyr Leu Lys Cys Glu Gly Ile Ile Ser Gly Asn Val 
            580                 585                 590         


Glu Ile Thr Gly Ile Asp Gly Asp Phe Lys Ala Ser Leu Thr Ala Tyr 
        595                 600                 605             


His Asp Phe Lys Glu Ile Leu Thr Gly Thr Glu Leu Ala Lys Lys Asp 
    610                 615                 620                 


Lys Glu Asn Ile Ile Thr Asn Ile Val Leu Phe Gly Asp Asp Lys Lys 
625                 630                 635                 640 


Leu Leu Lys Lys Arg Leu Asn Arg Leu Tyr Pro Gln Ile Thr Pro Asn 
                645                 650                 655     


Gln Leu Lys Lys Ile Cys Ala Leu Ser Tyr Thr Gly Trp Gly Arg Phe 
            660                 665                 670         


Ser Lys Lys Phe Leu Glu Glu Ile Thr Ala Pro Asp Pro Glu Thr Gly 
        675                 680                 685             


Glu Val Trp Asn Ile Ile Thr Ala Leu Trp Glu Ser Asn Asn Asn Leu 
    690                 695                 700                 


Met Gln Leu Leu Ser Asn Glu Tyr Arg Phe Met Glu Glu Val Glu Thr 
705                 710                 715                 720 


Tyr Asn Met Gly Lys Gln Thr Lys Thr Leu Ser Tyr Glu Thr Val Glu 
                725                 730                 735     


Asn Met Tyr Val Ser Pro Ser Val Lys Arg Gln Ile Trp Gln Thr Leu 
            740                 745                 750         


Lys Ile Val Lys Glu Leu Glu Lys Val Met Lys Glu Ser Pro Lys Arg 
        755                 760                 765             


Val Phe Ile Glu Met Ala Arg Glu Lys Gln Glu Ser Lys Arg Thr Glu 
    770                 775                 780                 


Ser Arg Lys Lys Gln Leu Ile Asp Leu Tyr Lys Ala Cys Lys Asn Glu 
785                 790                 795                 800 


Glu Lys Asp Trp Val Lys Glu Leu Gly Asp Gln Glu Glu Gln Lys Leu 
                805                 810                 815     


Arg Ser Asp Lys Leu Tyr Leu Tyr Tyr Thr Gln Lys Gly Arg Cys Met 
            820                 825                 830         


Tyr Ser Gly Glu Val Ile Glu Leu Lys Asp Leu Trp Asp Asn Thr Lys 
        835                 840                 845             


Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met Asp Asp Ser 
    850                 855                 860                 


Leu Asn Asn Arg Val Leu Val Lys Lys Lys Tyr Asn Ala Ile Lys Ser 
865                 870                 875                 880 


Asp Lys Tyr Pro Leu Asn Glu Asn Ile Arg His Glu Arg Lys Gly Phe 
                885                 890                 895     


Trp Lys Ser Leu Leu Asp Gly Gly Phe Ile Ser Lys Glu Lys Tyr Glu 
            900                 905                 910         


Arg Leu Ile Arg Asn Thr Glu Leu Ser Pro Glu Glu Leu Ala Gly Phe 
        915                 920                 925             


Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ser Thr Lys Ala Val Ala 
    930                 935                 940                 


Glu Ile Leu Lys Gln Val Phe Pro Glu Ser Glu Ile Val Tyr Val Lys 
945                 950                 955                 960 


Ala Gly Thr Val Ser Arg Phe Arg Lys Asp Phe Glu Leu Leu Lys Val 
                965                 970                 975     


Arg Glu Val Asn Asp Leu His His Ala Lys Asp Ala Tyr Leu Asn Ile 
            980                 985                 990         


Val Val Gly Asn Ser Tyr Tyr Val  Lys Phe Thr Lys Asn  Ala Ser Trp 
        995                 1000                 1005             


Phe Ile  Lys Glu Asn Pro Gly  Arg Thr Tyr Asn Leu  Lys Lys Met 
    1010                 1015                 1020             


Phe Thr  Ser Gly Trp Asn Ile  Glu Arg Asn Gly Glu  Val Ala Trp 
    1025                 1030                 1035             


Glu Val  Gly Lys Lys Gly Thr  Ile Val Thr Val Lys  Gln Ile Met 
    1040                 1045                 1050             


Asn Lys  Asn Asn Ile Leu Val  Thr Arg Gln Val His  Glu Ala Lys 
    1055                 1060                 1065             


Gly Gly  Leu Phe Asp Gln Gln  Ile Met Lys Lys Gly  Lys Gly Gln 
    1070                 1075                 1080             


Ile Ala  Ile Lys Glu Thr Asp  Glu Arg Leu Ala Ser  Ile Glu Lys 
    1085                 1090                 1095             


Tyr Gly  Gly Tyr Asn Lys Ala  Ala Gly Ala Tyr Phe  Met Leu Val 
    1100                 1105                 1110             


Glu Ser  Lys Asp Lys Lys Gly  Lys Thr Ile Arg Thr  Ile Glu Phe 
    1115                 1120                 1125             


Ile Pro  Leu Tyr Leu Lys Asn  Lys Ile Glu Ser Asp  Glu Ser Ile 
    1130                 1135                 1140             


Ala Leu  Asn Phe Leu Glu Lys  Gly Arg Gly Leu Lys  Glu Pro Lys 
    1145                 1150                 1155             


Ile Leu  Leu Lys Lys Ile Lys  Ile Asp Thr Leu Phe  Asp Val Asp 
    1160                 1165                 1170             


Gly Phe  Lys Met Trp Leu Ser  Gly Arg Thr Gly Asp  Arg Leu Leu 
    1175                 1180                 1185             


Phe Lys  Cys Ala Asn Gln Leu  Ile Leu Asp Glu Lys  Ile Ile Val 
    1190                 1195                 1200             


Thr Met  Lys Lys Ile Val Lys  Phe Ile Gln Arg Arg  Gln Glu Asn 
    1205                 1210                 1215             


Arg Glu  Leu Lys Leu Ser Asp  Lys Asp Gly Ile Asp  Asn Glu Val 
    1220                 1225                 1230             


Leu Met  Glu Ile Tyr Asn Thr  Phe Val Asp Lys Leu  Glu Asn Thr 
    1235                 1240                 1245             


Val Tyr  Arg Ile Arg Leu Ser  Glu Gln Ala Lys Thr  Leu Ile Asp 
    1250                 1255                 1260             


Arg Gln  Lys Glu Phe Glu Arg  Leu Ser Leu Glu Asp  Lys Ser Ser 
    1265                 1270                 1275             


Thr Leu  Phe Glu Ile Leu His  Ile Phe Gln Cys Gln  Ser Ser Ala 
    1280                 1285                 1290             


Ala Asn  Leu Lys Met Ile Gly  Gly Pro Gly Lys Ala  Gly Ile Leu 
    1295                 1300                 1305             


Val Met  Asn Asn Asn Ile Ser  Lys Cys Asn Lys Ile  Ser Ile Ile 
    1310                 1315                 1320             


Asn Gln  Ser Pro Thr Gly Ile  Phe Glu Asn Glu Ile  Asp Leu Leu 
    1325                 1330                 1335             


Lys Ile  
    1340 


<210>  876
<211>  1341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  876

Met Arg Phe Gln Asn Lys Lys Thr Gly Asp Tyr Phe Val Gly Leu Asp 
1               5                   10                  15      


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Thr Asn Glu Lys Tyr Glu 
            20                  25                  30          


Ile Leu Arg Ala Lys Asn His Arg Met Trp Gly Ser Arg Leu Phe Glu 
        35                  40                  45              


Glu Gly Lys Thr Ala Glu Glu Arg Arg Val His Arg Ala Gly Arg Arg 
    50                  55                  60                  


Arg Leu Asn Arg Arg Asn Asp Arg Leu Met Ile Leu Glu Asp Leu Phe 
65                  70                  75                  80  


Ala Glu Glu Ile Ser Lys Val Asp Lys Ala Phe Tyr Gln Arg Leu His 
                85                  90                  95      


Glu Ser Gln Tyr Arg Gln Glu His Lys Thr Val Ser Gly Pro Tyr Phe 
            100                 105                 110         


Leu Phe Asn Asp Lys Glu Phe Thr Asp Glu Asp Tyr His Ile Asp Tyr 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Val Ala Leu Met Lys Ala Pro Ala Lys 
    130                 135                 140                 


Asp Val Arg Leu Leu Phe Leu Ala Ile His His Ile Ile Lys Tyr Arg 
145                 150                 155                 160 


Gly Asn Phe Leu His Glu Gly Glu Thr Phe Lys Thr Thr Gly Gly Leu 
                165                 170                 175     


Thr Asp Ile Leu Gln Arg Val Leu Ala Cys Asn Glu Phe Ser Gly Leu 
            180                 185                 190         


Lys Gln His Ile Asp Asp Thr Ala Leu Val Lys Leu Leu Thr Asn Lys 
        195                 200                 205             


Gly Ile Thr Arg Ser Asp Ile Ala Lys Gln Ile Ser Glu Leu Ala Thr 
    210                 215                 220                 


Asp Lys Leu Tyr Arg Lys Gln Ala Lys Ala Phe Ala Ser Leu Val Leu 
225                 230                 235                 240 


Gly Leu Lys Ala Asn Leu Asp Asp Leu Phe Asn Glu Pro Glu Tyr Lys 
                245                 250                 255     


Glu Leu Asp Ser Glu Ile Arg Thr Val Glu Phe Ser Gly Lys Glu Tyr 
            260                 265                 270         


Trp Glu Glu Arg Asp Lys Tyr Glu Pro Tyr Leu Gly Glu Ser Leu Phe 
        275                 280                 285             


Ala Leu Asp Gly Cys Lys Thr Leu Tyr Asp Thr Leu Leu Leu Ser Asn 
    290                 295                 300                 


Ile Lys Pro Asp Gly Lys Tyr Leu Ser Glu Ala Lys Val Glu Thr Phe 
305                 310                 315                 320 


Glu Lys His His Glu His Leu Glu Ser Leu Lys Asp Leu Leu Lys Ser 
                325                 330                 335     


Asn Lys Ala Leu Tyr Asn Arg Val Leu Arg Asp Asp Glu Lys Asn Leu 
            340                 345                 350         


Asn Asn Tyr Ile Thr Tyr Ile Gly Lys Ser Glu Phe Asn Lys Arg Cys 
        355                 360                 365             


Ser Arg Asp Glu Phe Tyr Lys Phe Leu Lys Gln Thr Ile Lys Asp Ile 
    370                 375                 380                 


Ser Asp Asn Glu Asn Lys Ala Glu Ile Val Gln Leu Met Glu Leu Asp 
385                 390                 395                 400 


Ser Phe Leu Pro Leu Leu Arg Val Arg Glu Asn Gly Val Ile Pro Tyr 
                405                 410                 415     


Gln Ile His Glu Glu Glu Leu Glu Gln Ile Leu Asn Val Ser Ser Glu 
            420                 425                 430         


Lys Tyr Pro Phe Leu Asn Lys Ala Asp Gly Ser Gly Phe Thr Ile Ala 
        435                 440                 445             


Gln Lys Ile Leu Lys Leu Leu Thr Phe Arg Ile Pro Tyr Phe Val Gly 
    450                 455                 460                 


Pro Leu Asn Pro Ala His Lys Asp Arg Asp Asn Gly Phe Ala Trp Val 
465                 470                 475                 480 


Val Arg Lys Ala Pro Gly Lys Val Tyr Pro Trp Asn Phe Glu Asp Lys 
                485                 490                 495     


Val Asp Ile Ser Ala Ser Ala Thr Ala Phe Ile Glu Asn Leu Thr Asn 
            500                 505                 510         


Lys Cys Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Tyr Ser Leu 
        515                 520                 525             


Ala Tyr Ser Lys Phe Ser Leu Leu Asn Glu Leu Asn Asn Leu Arg Tyr 
    530                 535                 540                 


Asp Gly Lys Pro Leu Ser Tyr Glu Thr Lys Gln Ser Phe Ile Lys Asp 
545                 550                 555                 560 


Cys Phe Glu Lys Glu His Arg Lys Met Thr Glu Lys Arg Ile Arg Glu 
                565                 570                 575     


Phe Leu Lys His Leu Gly Gly Thr Asp Gly Lys Gly Ala Leu Thr Gly 
            580                 585                 590         


Leu Asp Val Asp Ile Lys Ser Asp Leu Lys Ser Tyr Arg Asp Met Val 
        595                 600                 605             


Arg Ile Leu Gly Asp Gly Phe Asp Glu Thr Met Ala Glu Arg Ile Ile 
    610                 615                 620                 


Arg Tyr Val Thr Leu Phe Gly Glu Ala Lys Lys Val Leu Leu Ser Thr 
625                 630                 635                 640 


Leu Glu Ile Glu Phe Gly Asp Asp Leu Ser Asp Thr Gln Lys Lys Gln 
                645                 650                 655     


Ile Leu Lys Leu Gln Tyr Arg Asp Trp Gly Arg Leu Ser Ala Thr Leu 
            660                 665                 670         


Leu Lys Gly Ile Lys Gly Val Ala Gly Asp Asn Pro Glu Cys Ser Ile 
        675                 680                 685             


Leu Glu Cys Met Glu Gln Gln Pro Leu Asn Leu Met Glu Val Leu Ser 
    690                 695                 700                 


Ser His Asn Thr Tyr Leu Ala Ala Ile Glu Glu Phe Asn Lys Glu Arg 
705                 710                 715                 720 


Thr Glu Pro Thr Asp Ile Val Asp Tyr Lys Leu Val Asp Asp Leu Tyr 
                725                 730                 735     


Ile Ser Pro Ala Val Lys Arg Ser Val Trp Gln Thr Leu Arg Ile Leu 
            740                 745                 750         


Asp Glu Ile Thr Lys Leu Arg Gly Ser Val Pro Lys Lys Val Phe Val 
        755                 760                 765             


Glu Val Ala Arg Thr Asn Arg Ala Glu Lys Lys Lys Thr Asp Ser Arg 
    770                 775                 780                 


Lys Glu Arg Leu Leu Gln Leu Tyr Lys Thr Ile Lys Asp Glu Gly Arg 
785                 790                 795                 800 


Asn Trp Thr Glu Glu Ile Lys Gln Arg Asp Ala Gly Asp Phe Asn Ser 
                805                 810                 815     


Lys Lys Leu Phe Leu Tyr Tyr Thr Gln Gln Gly Arg Cys Met Tyr Ser 
            820                 825                 830         


Gly Glu Lys Ile Thr Ser Ile Gly Asp Leu Phe Thr Ser Lys Tyr Asp 
        835                 840                 845             


Ile Asp His Ile Tyr Pro Arg Ser Val Thr Lys Asp Asn Ser Trp Asp 
    850                 855                 860                 


Asn Leu Val Leu Val Lys Ala Glu Leu Asn Arg Glu Lys Lys Asp Ile 
865                 870                 875                 880 


Tyr Pro Ile Glu Lys Asp Thr Gln Asp Arg Cys Ile Ser Leu Trp Lys 
                885                 890                 895     


Gln Leu Leu Glu Gln Gly Leu Ile Ser Glu Arg Lys Tyr Glu Arg Leu 
            900                 905                 910         


Val Arg Thr Thr Pro Leu Thr Ala Asp Glu Leu Ala Asp Phe Val Asn 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Asn Gln Ser Val Lys Ala Val Thr Asn Leu 
    930                 935                 940                 


Leu Lys Arg Leu Tyr Pro Asp Thr Thr Ile Cys Tyr Val Lys Ala Glu 
945                 950                 955                 960 


Asn Val Ser Asp Phe Arg Gln Glu Arg Glu Phe Val Lys Leu Arg Ser 
                965                 970                 975     


Leu Asn Ala His His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val 
            980                 985                 990         


Gly Asn Val Tyr His Glu Lys Phe  Thr Asn Asn Pro Arg  Asn Phe Ile 
        995                 1000                 1005             


Lys Asn  Ala Arg Ser Asp Lys  Lys Arg Pro Tyr Ser  Leu Asn His 
    1010                 1015                 1020             


Met Phe  Asp Tyr Leu Leu Gln  Val Gly Asn Lys Glu  Ile Trp Asn 
    1025                 1030                 1035             


Pro Lys  Thr Asp Asn Asn Leu  Val Lys Lys Met Met  Arg Asn Asn 
    1040                 1045                 1050             


Asp Val  Gln Val Thr Arg Met  Leu Val Glu Gln Thr  Gly Ala Leu 
    1055                 1060                 1065             


Tyr Asp  Ala Thr Ile Tyr Lys  Ala Ser Val Thr Lys  Ser Glu Ser 
    1070                 1075                 1080             


Tyr Val  Pro Leu Lys Ser Ser  Asp Ala Ile Leu Ser  Asp Val Lys 
    1085                 1090                 1095             


Lys Tyr  Gly Gly Phe Thr Ser  Ile Lys Ile Ala Tyr  Tyr Ser Ile 
    1100                 1105                 1110             


Phe Arg  Phe Tyr Lys Lys Asn  Ser Glu Glu Phe Glu  Thr His Ile 
    1115                 1120                 1125             


Ile Ser  Ile Pro Val Leu Lys  Ala Gly Thr Leu Asn  Ser Lys Glu 
    1130                 1135                 1140             


Asp Tyr  Val Ala Tyr Gly Glu  Glu Ile Met Asn Lys  Gln Lys Phe 
    1145                 1150                 1155             


Asp Arg  Val Glu Leu Val Tyr  Lys Lys Leu Cys Met  Asn Ser Leu 
    1160                 1165                 1170             


Val Arg  Ile Asn Gly Val Met  Tyr Tyr Leu Gly Gly  Lys Thr Asn 
    1175                 1180                 1185             


Asn Ser  Leu Tyr Ile Asp Ser  Ala Ile Ser Leu Val  Leu Pro Asp 
    1190                 1195                 1200             


Asn Ile  Ala Lys Lys Ile Lys  Ala Val Glu Lys Tyr  Ile Ala Arg 
    1205                 1210                 1215             


Val Ala  Glu Asn Gly Asn Tyr  Lys Pro Gln Pro Asp  Gln Val Ser 
    1220                 1225                 1230             


Pro Glu  Phe Asn Met Ile Ile  Tyr Lys Ala Leu Thr  Glu Lys Met 
    1235                 1240                 1245             


Lys Gln  Pro Ala Phe Val Gly  Leu Lys Thr Asn Arg  Glu Asn Glu 
    1250                 1255                 1260             


Leu Glu  Lys Glu Thr Thr Ile  Thr Ala Phe Ile Ala  Met Thr Leu 
    1265                 1270                 1275             


Ile Glu  Gln Cys Lys Met Leu  Met Glu Ile Leu Asn  Leu Leu Thr 
    1280                 1285                 1290             


Gln Gln  Lys Thr Thr Tyr Ala  Leu Lys Pro Ile Gly  Val Ser Ala 
    1295                 1300                 1305             


Ser Arg  Ser Thr Leu Gly Met  Asn Ile Thr Gly Leu  Ser Glu Phe 
    1310                 1315                 1320             


Thr Ile  Ile Thr Gln Ser Val  Thr Gly Leu Gln Glu  Lys Glu Ile 
    1325                 1330                 1335             


Arg Ile  Ile 
    1340     


<210>  877
<211>  1341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  877

Met Trp Gly Ser Arg Leu Phe Glu Glu Ala Ser Thr Ala Gln Asp Arg 
1               5                   10                  15      


Arg Glu His Arg Ser Asn Arg Arg Arg Leu Glu Arg Arg Lys Tyr Arg 
            20                  25                  30          


Leu Asp Leu Leu Glu Glu Leu Phe Val Glu Glu Ile Asn Lys Ile Asp 
        35                  40                  45              


Pro Glu Phe Phe Ile Arg Leu Arg Glu Ser Lys Phe His Phe Glu Asp 
    50                  55                  60                  


Arg Ser Leu Lys Asn Thr Phe Ile Leu Phe Asn Asp Lys Met Glu Gly 
65                  70                  75                  80  


Gly Tyr Asn Asp Lys Val Phe Phe Ser Lys Tyr Pro Thr Ile Tyr His 
                85                  90                  95      


Leu Arg Ser Ala Leu Ile Asn Gly Asn Lys Glu Asp Val Arg Ala Val 
            100                 105                 110         


Phe Leu Ala Ile His Asn Ile Leu Lys Asn Arg Gly His Phe Leu Leu 
        115                 120                 125             


Ala Gly Asn Asp Ile Asn Ile Ser Leu Ser Leu Asn Asp Thr Leu Thr 
    130                 135                 140                 


Asp Leu Phe Glu Tyr Leu Lys Ile His Leu Gly Ile Glu Val Glu Phe 
145                 150                 155                 160 


Glu Asn Lys Leu Lys Asp Ile Glu Lys Ile Ala Leu Asp Lys Asn Leu 
                165                 170                 175     


Ser Lys Ser Asp Lys Gln Lys Lys Ile Val Gln Leu Leu Glu Thr Asp 
            180                 185                 190         


Tyr Lys Lys Gln Leu Ala Asp Val Phe Lys Leu Ser Leu Gly Leu Thr 
        195                 200                 205             


Ala Asn Met Glu Asn Ile Phe Gly Lys Glu Asn Glu Gln Gly Leu Glu 
    210                 215                 220                 


Phe Ile Asn Ala Glu Asn Lys Ile Lys Lys Ile Gln Phe Asp Ser Ser 
225                 230                 235                 240 


Tyr Glu Glu Asn Ile Ala Glu Phe Asp Gln Phe Tyr Gly Glu Tyr Phe 
                245                 250                 255     


Glu Ile Ile Asp Ile Cys Lys Lys Ile Tyr Asp Ala Ile Ser Leu Ser 
            260                 265                 270         


Gln Ile Leu Val Asp Gly Lys Gly Leu Ser Glu Ser Lys Val Leu Ser 
        275                 280                 285             


Tyr Glu Lys His Lys Glu Asp Leu Lys Leu Leu Lys Lys Val Leu Lys 
    290                 295                 300                 


Asn Ile Asp Lys Ala Tyr Asp Asn Lys Gly Glu Asp Arg Leu Val Tyr 
305                 310                 315                 320 


Lys Val Leu Lys Glu Asp Lys Asp Lys Gly Thr Asn Tyr Val Asn Tyr 
                325                 330                 335     


Ser Lys Leu Ser Asn Asn Gly Thr Ala Thr Thr Lys Glu Glu Phe Tyr 
            340                 345                 350         


Lys Phe Leu Ser Lys Asn Leu Asp Leu Lys Lys Phe Gly Lys Leu Asn 
        355                 360                 365             


Lys Glu Asp Ile Glu Thr Leu Asp Tyr Ile Asn Ser Glu Ile Leu Leu 
    370                 375                 380                 


Gly Arg Phe Leu Pro Leu Gln Lys Glu Lys Ile Asn Gly Val Ile Pro 
385                 390                 395                 400 


Tyr Gln Leu Gln Met Lys Glu Leu Thr Gln Ile Leu Asn Asn Ala Ser 
                405                 410                 415     


Lys Tyr Leu Pro Phe Leu Asn Lys Ile Glu Asp Gly Met Thr Val Lys 
            420                 425                 430         


Asp Lys Ile Ile Ser Leu Ile Lys Phe Arg Ile Pro Tyr Tyr Val Gly 
        435                 440                 445             


Pro Leu Asn Glu Tyr Asn Arg Ile Glu Asn Gly Asn Gly Phe Ala Trp 
    450                 455                 460                 


Ile Glu Lys Lys Lys Asn Glu Lys Ile Phe Pro Trp Asn Phe Asn Asp 
465                 470                 475                 480 


Val Val Asp Ile Glu Lys Ser Ala Glu Lys Phe Ile Asn Asn Leu Thr 
                485                 490                 495     


Asn Lys Cys Thr Tyr Ile Lys Glu Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         


Ile Ile Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Gln 
        515                 520                 525             


Tyr Gly Asn Lys Lys Leu Asp Ile Glu Leu Lys Met Lys Met Tyr Thr 
    530                 535                 540                 


Asp Leu Phe Leu Glu Lys Lys Asp Asn Arg Lys Val Thr Glu Lys Arg 
545                 550                 555                 560 


Ile Leu Glu Tyr Phe Arg Ile Asn Gly Ile Asn Asp Gly Lys Glu Lys 
                565                 570                 575     


Leu Thr Gly Leu Asn Asn Glu Ile His Ala Asp Met Lys Ser Tyr Arg 
            580                 585                 590         


Asp Met Cys Glu Ile Met Gly Glu Asn Phe Asn Ile Asn Val Ala Glu 
        595                 600                 605             


Asn Ile Ile Asn Trp Ile Thr Val Phe His Gly Glu Lys Lys Ile Leu 
    610                 615                 620                 


Ile Asn Lys Ile Lys Asn Val Tyr Gly Lys Met Phe Asn Asp Glu Gln 
625                 630                 635                 640 


Ile Ser Lys Leu Ser Lys Leu Asn Tyr Ser Asp Trp Gly Arg Leu Ser 
                645                 650                 655     


Lys Lys Leu Leu Thr Asp Ile Lys Cys Tyr Ile Asp Ala Asp Gly Ile 
            660                 665                 670         


Asn Phe Pro Val Ser Ile Leu Glu Ala Met Glu Val Ser Asn Lys Asn 
        675                 680                 685             


Leu Met Glu Leu Leu Ser Asn Lys Tyr Glu Phe Ile Ser Cys Ile Asp 
    690                 695                 700                 


Asp Ile Asn Asn Lys Tyr Phe Gly Asp Arg Asp Ile Ser Ala Ser Glu 
705                 710                 715                 720 


Leu Ile Asp Glu Leu Tyr Val Ser Pro Ala Val Lys Arg Gly Ile Trp 
                725                 730                 735     


Gln Ser Val Arg Ile Leu Glu Glu Ile Val Ser Ile Ile Gly His Asn 
            740                 745                 750         


Pro Lys Lys Ile Phe Ile Glu Met Thr Arg His Asn Glu Glu Lys Val 
        755                 760                 765             


Gln Lys Ser Ser Arg Arg His Asn Leu Glu Glu Leu Tyr Lys Ala Ile 
    770                 775                 780                 


Arg Lys Asp Pro Glu Tyr Val Glu Leu Ile Lys Glu Phe Asp Ser Thr 
785                 790                 795                 800 


Thr Asp Thr Asp Ser Phe Lys Ile Lys Lys Leu Tyr Leu Tyr Phe Met 
                805                 810                 815     


Gln Leu Gly Lys Cys Ala Tyr Ser Lys Glu Lys Ile Asp Phe Asp Lys 
            820                 825                 830         


Leu Phe Thr Asp Ser Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Lys 
        835                 840                 845             


Thr Lys Asp Asp Ser Ile His Asn Asn Leu Val Leu Val Lys Ala Lys 
    850                 855                 860                 


Tyr Asn Arg Glu Lys Thr Asp Val Tyr Pro Ile Asp Ile Asn Ile Gln 
865                 870                 875                 880 


Lys Asn Met Asn Ser Phe Trp Asn Phe Leu Tyr Thr Lys Lys Phe Ile 
                885                 890                 895     


Thr Glu Glu Lys Tyr Lys Arg Leu Ile Arg Thr Ser Asp Leu Thr Asp 
            900                 905                 910         


Glu Glu Leu Ala Lys Phe Ile Ser Arg Gln Leu Val Glu Thr Ser Gln 
        915                 920                 925             


Ser Thr Lys Ala Thr Ala Glu Val Leu Lys Gln Ile Tyr Gly Asp Ser 
    930                 935                 940                 


Asp Ile Cys Tyr Val Lys Ala Glu Asn Val Ser Asp Phe Arg Tyr Gly 
945                 950                 955                 960 


Ser Ser Arg Gln Lys Asp Lys Asn Asp Ile Gln Lys Leu Phe Val Lys 
                965                 970                 975     


Cys Arg Glu Val Asn His Phe His His Ala Lys Asp Ala Tyr Leu Asn 
            980                 985                 990         


Ile Val Val Gly Asn Val Tyr Asp  Val Lys Phe Thr Lys  Asn Pro Val 
        995                 1000                 1005             


Asn Phe  Ile Lys Lys Asn Lys  Met Gly Asn Lys Ser  Asn Tyr Ser 
    1010                 1015                 1020             


Leu Asn  His Met Phe Cys Phe  Asp Val Glu Arg Asn  Gly Tyr Lys 
    1025                 1030                 1035             


Ala Trp  Asp Ser Ser Glu Thr  Ile Lys Thr Val Gly  Arg Met Met 
    1040                 1045                 1050             


Lys Ser  Asn Asp Val Arg Ile  Thr Lys His Val His  Pro Thr Arg 
    1055                 1060                 1065             


Gly Glu  Leu Tyr Asp Ala Thr  Ile Tyr Lys Ala Lys  Ile Ala Lys 
    1070                 1075                 1080             


Thr Gly  Ser Tyr Met Pro Leu  Lys Ser Ser Lys Asn  Thr Pro Leu 
    1085                 1090                 1095             


Ser Asp  Val Thr Lys Tyr Gly  Gly Tyr Thr Lys Ile  Lys Ile Ala 
    1100                 1105                 1110             


Tyr Tyr  Ser Val Tyr Lys Tyr  Lys Phe Ser Asp Lys  Asn Leu Ile 
    1115                 1120                 1125             


Lys Thr  Glu Ile Arg Leu Ile  Pro Ile Pro Ile Tyr  Leu Ser Lys 
    1130                 1135                 1140             


Asn Asp  Asp Asp Ile Lys Glu  Tyr Val Lys Asn Tyr  Leu Asn Asp 
    1145                 1150                 1155             


Arg Val  Asn Ser Lys Leu Ile  Asp Phe Thr Lys Ile  Tyr Asp Lys 
    1160                 1165                 1170             


Leu Tyr  Val Asn Ser Leu Ile  Lys Ile Asn Gly Tyr  Leu Tyr Tyr 
    1175                 1180                 1185             


Leu Gly  Gly Lys Thr Asn Asp  Arg Val Trp Ile Asp  Asn Ala Val 
    1190                 1195                 1200             


Ser Ile  Val Leu Asp Asp Lys  Ile Glu Tyr Leu Ile  Lys Arg Ile 
    1205                 1210                 1215             


Ser Asn  Asp Ile Lys Tyr Gly  Tyr Glu Ile Asn Val  Ser Asp Asp 
    1220                 1225                 1230             


Glu Leu  Met Asp Ile Tyr Glu  Ser Leu Ala Glu Lys  Ala Asn Lys 
    1235                 1240                 1245             


Lys Ile  Phe Tyr Asn Arg Leu  Ser Asp Phe Glu Lys  Ile Phe Ser 
    1250                 1255                 1260             


Asn Met  Asp Val Lys Lys Lys  Phe Val Lys Leu Glu  Lys Asn Asp 
    1265                 1270                 1275             


Lys Cys  Arg Val Leu Leu Gln  Leu Leu Asn Val Leu  Thr Asp Ser 
    1280                 1285                 1290             


Gln Thr  Thr Tyr Lys Leu Glu  Met Val Gly Leu Lys  His Ser Arg 
    1295                 1300                 1305             


Ser Asn  Ile Gly Met Lys Ile  Ser Asn Leu Glu Glu  Phe Lys Val 
    1310                 1315                 1320             


Ile Asn  Ser Ser Ile Thr Gly  Leu Tyr Ser Asn Glu  Ile Asp Ile 
    1325                 1330                 1335             


Leu Asn  Ile 
    1340     


<210>  878
<211>  1341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  878

Met Glu Lys Asp Tyr Tyr Leu Gly Leu Asp Met Gly Ser Gly Ser Ala 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Asp Gln Tyr His Val Leu Arg Ala His Gly 
            20                  25                  30          


Lys Ala Met Trp Gly Thr Arg Leu Phe Glu Ser Ala Asn Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Ile Asn Arg Thr Ala Arg Arg Arg Leu Lys Arg Arg Asn 
    50                  55                  60                  


Gln Arg Ile Glu Trp Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Thr 
65                  70                  75                  80  


Lys Asp Ser Gly Phe Phe Arg Arg Met Lys Glu Ser Arg Tyr Trp Ala 
                85                  90                  95      


Glu Asp Lys Arg Asp Glu Asn Gly Ser Cys Pro Glu Met Pro Tyr Ser 
            100                 105                 110         


Leu Phe Ile Asp Glu Asp Phe Thr Asp Lys Glu Tyr His Glu Lys Phe 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Met Thr Thr Glu Glu Thr 
    130                 135                 140                 


Pro Gly Ile Arg Leu Val Tyr Leu Ala Phe His His Met Met Lys His 
145                 150                 155                 160 


Arg Gly His Phe Leu Leu Asn Gly Asn Ile Glu Glu Ile Arg Lys Phe 
                165                 170                 175     


Arg Lys Thr Phe Asp Gln Phe Leu Glu Val Val Arg Asn Glu Glu Met 
            180                 185                 190         


Gly Phe Ala Gly Glu Ile Glu Glu Glu Thr Tyr Lys Lys Ile Glu Met 
        195                 200                 205             


Thr Leu Lys Asp Val Asn Leu Thr Arg Ser Ala Lys Lys Thr Thr Leu 
    210                 215                 220                 


Ile Lys Ser Leu Lys Val Thr Arg Val Glu Glu Lys Glu Leu Leu Ala 
225                 230                 235                 240 


Leu Ile Ser Gly Cys Thr Val Lys Leu Ser Asn Ile Phe Asn Asp Lys 
                245                 250                 255     


Ser Leu Asp Glu Thr Glu Arg Pro Lys Ile Ser Phe Ala Asp Ala Gly 
            260                 265                 270         


Phe Asp Asp Tyr Glu Ala Glu Ile Gln Ser Asn Leu Asn Glu Lys Tyr 
        275                 280                 285             


Tyr Ile Ile Glu Ser Ala Lys Ala Val Tyr Asp Trp Ala Ala Leu Ala 
    290                 295                 300                 


Asp Ile Leu Gly Glu Tyr Ser Cys Ile Ser Glu Ala Lys Val Ala Leu 
305                 310                 315                 320 


Tyr Glu Lys His His Lys Asp Leu Lys Arg Leu Lys Asp Leu Val Arg 
                325                 330                 335     


Asn Asn Ile Ser Ser Glu Val Tyr Lys Glu Ile Phe Val Asp Val Ser 
            340                 345                 350         


Gly Lys Tyr Lys Asn Tyr Cys Ala Tyr Ile Gly Thr Thr Asn Ile Asn 
        355                 360                 365             


Gly Lys Lys Lys Asp Phe Glu Gly Lys Lys Cys Thr Lys Glu Asp Phe 
    370                 375                 380                 


Tyr Ala Tyr Leu Lys Lys Ser Val Ile Glu Cys Ile Asn Asp Ser Glu 
385                 390                 395                 400 


Lys Thr Asp Trp Leu Lys Leu Glu Leu Glu Lys Gly Asn Phe Leu Pro 
                405                 410                 415     


Lys Gln Leu Thr Gly Glu Asn Ser Val Ile Pro Tyr Gln Val His Leu 
            420                 425                 430         


Tyr Glu Leu Asn Ala Ile Ile Thr Asn Leu Gln Glu Arg Ile Pro Leu 
        435                 440                 445             


Leu Lys Arg Asp Gly Glu Lys Ile Arg Gln Ile Phe Thr Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Asn Gly Ile Ser Ile Asp Gly Lys Lys 
465                 470                 475                 480 


Thr Asn Trp Val Lys Arg Lys Glu Gly Val Ile Tyr Pro Trp Asn Phe 
                485                 490                 495     


Ser Asp Ile Val Asp Glu Glu Ser Ser Ala Glu Ser Phe Ile Arg Arg 
            500                 505                 510         


Met Thr Asn Lys Cys Thr Tyr Leu Ile His Glu Asp Val Leu Pro Lys 
        515                 520                 525             


Tyr Ser Leu Leu Tyr Ser Arg Phe Glu Val Leu Asn Glu Leu Asn Asn 
    530                 535                 540                 


Val Arg Leu Asn Gly Glu Leu Leu Ser Val Glu Leu Lys Gln Lys Ile 
545                 550                 555                 560 


Tyr Glu Asp Val Phe Gln Arg Tyr Arg Lys Val Thr Gln Lys Arg Leu 
                565                 570                 575     


Lys Arg Tyr Leu Leu Thr Glu Gly Ile Ile Asp Glu Asn Thr Val Ile 
            580                 585                 590         


Thr Gly Leu Asp Gly Asp Phe Lys Gly Ser Leu Thr Ala Tyr His Asp 
        595                 600                 605             


Phe Lys Glu Lys Leu Thr Gly Leu Asn Leu Thr Glu Arg Glu Lys Glu 
    610                 615                 620                 


Glu Ile Ile Leu Asn Ile Thr Leu Phe Gly Asp Ala Arg Ser Leu Leu 
625                 630                 635                 640 


Arg Lys Arg Ile Gly Lys Ile Cys Ser Gln Leu Ser Asp Lys Gln Lys 
                645                 650                 655     


Glu Asn Leu Ser Lys Leu Ser Tyr Gln Gly Trp Gly Arg Leu Ser Lys 
            660                 665                 670         


Lys Phe Leu Asn Gly Ile Ala Ile Ser Val Gln Gly Glu Glu Glu Trp 
        675                 680                 685             


Thr Ile Ile Lys Ala Leu Trp Glu Thr Gln Asp Asn Leu Ser Gln Leu 
    690                 695                 700                 


Met Gly Ala Lys Tyr Thr Phe Arg Asn Ala Val Glu Lys Glu Asn Gly 
705                 710                 715                 720 


Glu Leu Glu Asn Arg Glu Ile Ser Tyr Glu Thr Val Lys Asp Leu Ser 
                725                 730                 735     


Ile Ser Pro Ser Val Lys Arg Gln Val Trp Gln Ser Leu Leu Ile Val 
            740                 745                 750         


Lys Glu Ile Cys Lys Ile Gln Lys Lys Pro Pro Arg Lys Val Phe Val 
        755                 760                 765             


Glu Val Ala Lys Glu Lys Thr Asn Ser Gly Arg Thr Glu Ser Arg Lys 
    770                 775                 780                 


Lys Arg Leu Gln Asp Phe Tyr Lys Tyr Cys Ser Lys Tyr Glu Lys Glu 
785                 790                 795                 800 


Leu Tyr Asp Glu Ser Ile Gln Asn Lys Leu Glu Lys Cys Ser Glu Ser 
                805                 810                 815     


Asp Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Met Gln Met Gly Arg 
            820                 825                 830         


Cys Met Tyr Thr Gly Gln Pro Ile Asp Phe Asp Glu Leu Trp Asn Asn 
        835                 840                 845             


Gln Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met Asp 
    850                 855                 860                 


Asp Ser Ile Asp Asn Arg Val Leu Val Lys Arg Ile Ser Asn Gln Glu 
865                 870                 875                 880 


Lys Thr Asp Val Tyr Pro Ile Arg Ala Asp Ile Arg Glu Lys Arg Arg 
                885                 890                 895     


Gly Phe Trp Lys Glu Leu Lys Asn Val Gly Leu Ile Ser Glu Glu Lys 
            900                 905                 910         


Tyr Lys Arg Leu Thr Arg Ala Thr Glu Phe Glu Pro Ala Glu Leu Ala 
        915                 920                 925             


Gly Phe Ile Ser Arg Gln Leu Val Glu Asn Arg Gln Thr Thr Lys Val 
    930                 935                 940                 


Val Ala Ser Ile Leu Lys Gln Ala Met Pro Asp Thr Glu Ile Val Tyr 
945                 950                 955                 960 


Val Lys Ala Arg Thr Val Ser Glu Phe Arg His Lys Phe Asn Phe Ile 
                965                 970                 975     


Lys Val Arg Glu Met Asn Asp Phe His His Ala Lys Asp Ala Tyr Leu 
            980                 985                 990         


Asn Val Val Val Gly Asn Thr Tyr  Leu Val Lys Phe Thr  Lys Ser Pro 
        995                 1000                 1005             


Val Asn  Phe Ile Glu Asn His  Ser Asn Arg Ser Tyr  Asn Leu Lys 
    1010                 1015                 1020             


Lys Met  Phe Thr Asp Gly Arg  Val Glu Arg Asn Gly  Glu Ile Ala 
    1025                 1030                 1035             


Trp Ile  Ser Gly Glu Asn Gly  Thr Ile Gly Thr Val  Arg Asn Ile 
    1040                 1045                 1050             


Met Lys  Lys Asn Asn Ile Leu  Val Thr Arg Arg Ala  His Glu Ala 
    1055                 1060                 1065             


Thr Gly  Gly Phe Phe Asp Gln  Gln Leu Met Lys Lys  Gly Lys Gly 
    1070                 1075                 1080             


Gln Val  Pro Val Lys Gly Ser  Asp Glu Arg Leu Leu  Asp Ile Glu 
    1085                 1090                 1095             


Lys Tyr  Gly Gly Tyr Asn Lys  Glu Thr Gly Ala Tyr  Phe Val Leu 
    1100                 1105                 1110             


Val Glu  Ser Asp Glu Lys Lys  Gly Lys Lys Lys Arg  Thr Ile Glu 
    1115                 1120                 1125             


Tyr Val  Pro Ile Arg Leu Lys  Lys Gln Leu Glu Asn  Asn Arg Lys 
    1130                 1135                 1140             


Ala Leu  Glu Asn Tyr Leu Cys  Glu Gln Arg Gly Leu  Thr Asn Pro 
    1145                 1150                 1155             


Val Val  Leu Ile Asp Lys Ile  Lys Met Asp Ser Leu  Phe Arg Val 
    1160                 1165                 1170             


Asp Gly  Phe Phe Met Trp Leu  Ser Gly Arg Thr Gly  Asn Gln Leu 
    1175                 1180                 1185             


Leu Phe  Lys Cys Ala Asn Gln  Leu Val Leu Ser Thr  Glu Asp Glu 
    1190                 1195                 1200             


Ala Thr  Leu Lys Lys Ile Val  Lys Phe Val Asn Arg  Arg Lys Glu 
    1205                 1210                 1215             


Asn Lys  Glu Ile Lys Ile Tyr  Glu Ser Asp Lys Leu  Asn Glu Glu 
    1220                 1225                 1230             


Met Leu  Asn Arg Leu Tyr Asn  Thr Phe Leu Tyr Lys  Leu Gln Asn 
    1235                 1240                 1245             


Ser Ile  Tyr Gly Asn Arg Leu  Gly Glu Gln Ala Lys  Thr Leu Ser 
    1250                 1255                 1260             


Ser Lys  Lys Glu Asn Phe Asp  Lys Leu Gln Ile Glu  Glu Lys Cys 
    1265                 1270                 1275             


Leu Val  Leu Tyr Glu Ile Leu  His Met Phe Gln Cys  Gln Ser Ala 
    1280                 1285                 1290             


Thr Ser  Asp Leu Gln Leu Ile  Glu Gly Pro Gly Lys  Ala Gly Thr 
    1295                 1300                 1305             


Leu Arg  Leu Asn Ser Asn Ile  Thr Lys Tyr Lys Asn  Ile Asn Ile 
    1310                 1315                 1320             


Ile Asn  Gln Ser Pro Thr Gly  Ile Tyr Glu Gln Val  Ile Asp Leu 
    1325                 1330                 1335             


Gln Lys  Ile 
    1340     


<210>  879
<211>  1341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  879

Met Gly Lys Gly Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Glu Ser Tyr Gln Ile Leu Arg Arg His Gly 
            20                  25                  30          


Lys Ala Met Trp Gly Val Arg Leu Phe Glu Ser Ala Lys Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Met Phe Arg Thr Gly Arg Arg Arg Leu Asp Arg Arg Gly 
    50                  55                  60                  


Trp Arg Ile Glu Ile Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Arg 
65                  70                  75                  80  


Val Asp Pro Gly Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro 
                85                  90                  95      


Glu Asp Lys Arg Asp Ile Gln Gly Asn Cys Pro Glu Leu Pro Tyr Thr 
            100                 105                 110         


Leu Phe Val Asp Lys Thr Phe Thr Asp Lys Asp Phe His Lys Lys Tyr 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu Thr Glu Asp Thr 
    130                 135                 140                 


Pro Asp Met Arg Leu Val Tyr Leu Ala Leu His His Met Met Lys His 
145                 150                 155                 160 


Arg Gly His Phe Leu Leu Ser Gly Asp Ile Ser Gln Val Thr Glu Phe 
                165                 170                 175     


Lys Asn Thr Phe Asn Gln Phe Ile Glu Asn Ile Arg Asn Glu Glu Met 
            180                 185                 190         


Asp Phe Glu Ile Glu Leu Asp Glu Ser Ala Val Gln Met Ile Glu Glu 
        195                 200                 205             


Thr Leu Lys Asp Lys Asn Leu Thr Arg Ser Ala Lys Lys Ser Lys Leu 
    210                 215                 220                 


Val Lys Gly Leu Asn Ala Lys Asn Val Arg Asp Lys Ala Phe Leu Thr 
225                 230                 235                 240 


Leu Leu Ser Gly Gly Thr Val Lys Leu Ser Asp Leu Phe Gly Met Glu 
                245                 250                 255     


Glu Leu Asn Glu Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Asn Gly 
            260                 265                 270         


Tyr Glu Glu Tyr Ala Ala Val Val Glu Met Glu Leu Gly Glu Leu Tyr 
        275                 280                 285             


Tyr Ile Val Glu Ser Ala Lys Ala Val Tyr Asp Trp Ala Ile Leu Ser 
    290                 295                 300                 


Asp Ile Leu Gly Gly Ser Thr Ser Val Ser Asp Ala Lys Val Arg Ala 
305                 310                 315                 320 


Tyr Glu Lys His Lys Ala Asp Leu Lys Tyr Leu Lys Ala Val Val Lys 
                325                 330                 335     


Glu Tyr Phe Pro Lys Glu Val Tyr Asn Ala Val Phe Val Glu Ser Ser 
            340                 345                 350         


Asp Lys Leu Asn Asn Tyr Pro Ala Tyr Ile Gly Met Thr Lys Lys Asn 
        355                 360                 365             


Gly Lys Lys Val Ser Leu Glu Gly Lys Arg Cys Ser Arg Glu Glu Phe 
    370                 375                 380                 


Met Asp Phe Leu Lys Lys Asn Ile Val Val Lys Leu Pro Asp Glu Glu 
385                 390                 395                 400 


Ala Lys Met Tyr Leu Gln Ser Glu Leu Glu Lys Asp Ser Phe Leu Pro 
                405                 410                 415     


Lys Gln Val Asn Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Lys 
            420                 425                 430         


Tyr Glu Leu Lys Lys Ile Leu Asp Asn Leu Gly Asp Lys Ile Pro Phe 
        435                 440                 445             


Leu Lys Glu Asn Ala Glu Lys Ile Glu Lys Leu Phe Ser Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Arg Thr Gly Asn Gly Glu Asn Ser Lys 
465                 470                 475                 480 


Phe Ala Trp Ala Glu Arg Lys Ser Thr Glu Lys Ile Tyr Pro Trp Asn 
                485                 490                 495     


Phe Glu Asn Val Ile Asp Val Glu Gln Ser Ala Glu Asn Phe Ile Arg 
            500                 505                 510         


Arg Met Thr Asn Lys Cys Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro 
        515                 520                 525             


Lys Asp Ser Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn 
    530                 535                 540                 


Asn Leu Arg Leu Asp Gly Gln Lys Ile Ser Val Glu Leu Lys Gln Lys 
545                 550                 555                 560 


Ile Tyr Arg Asp Val Phe Cys Arg Ser Arg Lys Val Thr Gln Lys Lys 
                565                 570                 575     


Leu Lys Ser Tyr Leu Ile Arg Glu Gly Ile Ala Gly Lys Asn Val Glu 
            580                 585                 590         


Leu Thr Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Thr Ala Tyr His 
        595                 600                 605             


Asp Phe Lys Glu Lys Leu Ile Asp Val Glu Leu Thr Gln Lys Glu Lys 
    610                 615                 620                 


Glu Glu Ile Ile Leu Asn Ile Val Leu Phe Gly Asp Asp Lys Lys Leu 
625                 630                 635                 640 


Leu Lys Gln Arg Leu Lys Lys Lys Phe Pro Gln Leu Thr Glu Lys Gln 
                645                 650                 655     


Ile Asn Thr Val Thr Ala Leu Ser Tyr Lys Gly Trp Gly Arg Leu Ser 
            660                 665                 670         


Lys Lys Leu Leu Glu Glu Val Thr Val Pro Ala Pro Glu Thr Gly Glu 
        675                 680                 685             


Val Trp Asn Ile Ile Thr Ala Leu Trp Glu Thr Asn Asp Asn Phe Met 
    690                 695                 700                 


Gln Leu Leu Ser Lys Glu Tyr Gln Phe Ser Asp Arg Ile Glu Glu Phe 
705                 710                 715                 720 


Asn Glu Glu Lys Ala Asp Pro Glu Val Ser Tyr Gln Thr Ile Glu Asp 
                725                 730                 735     


Leu Tyr Val Ser Pro Ala Val Lys Arg Gln Ile Trp Gln Thr Leu Gln 
            740                 745                 750         


Ile Ile Gln Glu Ile Glu Lys Val Met Gly Glu Lys Pro Lys Arg Val 
        755                 760                 765             


Phe Val Glu Met Ala Arg Glu Lys Gln Glu Asn Lys Arg Thr Glu Ser 
    770                 775                 780                 


Arg Lys Lys Thr Leu Ser Asp Leu Tyr Thr Lys Cys Lys Lys Glu Glu 
785                 790                 795                 800 


Pro Glu Trp Ile Glu Ala Leu Cys Thr Ser Leu Glu Lys His Glu Glu 
                805                 810                 815     


His Gln Leu Arg Ser Asp Lys Leu Tyr Leu Tyr Tyr Thr Gln Lys Gly 
            820                 825                 830         


Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp Leu Trp Asp 
        835                 840                 845             


Asn Thr Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met 
    850                 855                 860                 


Asp Asp Ser Leu Lys Asn Arg Val Leu Val Lys Lys Glu Tyr Asn Ala 
865                 870                 875                 880 


Lys Lys Ser Asp Thr Tyr Pro Ile Ala Ala Asp Ile Gln Lys Lys Met 
                885                 890                 895     


Met Pro Phe Trp Lys Ser Leu Leu Thr Gly Gly Phe Ile Pro Lys Glu 
            900                 905                 910         


Lys Tyr Asp Arg Leu Val Arg Asn Asn Pro Leu Asp Ala Asn Glu Leu 
        915                 920                 925             


Ala Gly Phe Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ser Thr Lys 
    930                 935                 940                 


Ala Val Ala Glu Ile Leu Lys Lys Ile Leu Pro Asp Thr Glu Ile Val 
945                 950                 955                 960 


Tyr Val Lys Ala Lys Thr Val Ser Lys Phe Arg Gln Asp Phe Asp Phe 
                965                 970                 975     


Ile Lys Val Arg Asp Met Asn Asp Leu His His Ala Lys Asp Ala Tyr 
            980                 985                 990         


Leu Asn Ile Val Val Gly Asn Val  Tyr Phe Val Lys Phe  Thr Lys Asn 
        995                 1000                 1005             


Ala Ala  Trp Phe Val Lys Glu  Asn Pro Gly Arg Thr  Tyr Asn Leu 
    1010                 1015                 1020             


Glu Lys  Met Phe Ile Trp Asp  Val Ala Arg Asp Gly  Glu Ile Ala 
    1025                 1030                 1035             


Trp Lys  Ala Gly Lys Ser Gly  Ser Ile Val Gln Val  Arg Ala Met 
    1040                 1045                 1050             


Met Glu  Lys Asn His Ile Leu  Val Thr Arg Arg Ser  Tyr Glu Val 
    1055                 1060                 1065             


Lys Gly  Gly Leu Phe Asp Gln  Gln Ile Leu Lys Lys  Gly Lys Gly 
    1070                 1075                 1080             


Gln Val  Pro Val Lys Glu Ser  Asp Glu Arg Leu Arg  Asp Ile Gly 
    1085                 1090                 1095             


Lys Tyr  Gly Gly Tyr Asn Lys  Ala Ala Gly Val Tyr  Phe Met Leu 
    1100                 1105                 1110             


Val Ser  Ser Lys Asp Lys Lys  Gly Lys Glu Gln Arg  Thr Ile Glu 
    1115                 1120                 1125             


Phe Val  Pro Ile Tyr Leu Lys  Asp Arg Val Glu Lys  Asp Met Glu 
    1130                 1135                 1140             


Ser Ala  Arg Asn Tyr Leu Met  Glu Glu Arg Gly Leu  Arg Glu Pro 
    1145                 1150                 1155             


Lys Ile  Leu Ile Lys Lys Ile  Lys Ile Asp Thr Leu  Phe Lys Val 
    1160                 1165                 1170             


Asp Gly  Phe Tyr Met Trp Leu  Ser Gly Arg Thr Ser  Asn Gln Leu 
    1175                 1180                 1185             


Ile Phe  Lys Gly Ala Asn Gln  Leu Ile Leu Ser Lys  Glu Asp Met 
    1190                 1195                 1200             


Lys Thr  Leu Lys Lys Val Val  Lys Tyr Ile Gln Arg  Gln Lys Glu 
    1205                 1210                 1215             


Asp Lys  Asn Val Lys Ile Ser  Met Arg Asp Gly Ile  Thr Glu Glu 
    1220                 1225                 1230             


Glu Leu  Leu Lys Leu Tyr Glu  Thr Phe Leu Asp Lys  Ile Arg His 
    1235                 1240                 1245             


Ser Ile  Tyr Gly Ala Arg Leu  Ser Ser Gln Glu Lys  Thr Leu Val 
    1250                 1255                 1260             


Asn Gly  Lys Glu Lys Phe Ile  Asp Leu Ser Val Glu  Ala Lys Cys 
    1265                 1270                 1275             


Gln Val  Leu Tyr Glu Ile Leu  His Leu Phe Gln Cys  Gln Ser Gly 
    1280                 1285                 1290             


Ala Ala  Asn Leu Lys Met Ile  Gly Gly Pro Ala Ser  Ala Gly Ile 
    1295                 1300                 1305             


Leu Val  Met Asn Asn Asn Ile  Ser Lys Cys Lys Asn  Ile Ser Ile 
    1310                 1315                 1320             


Val Asn  Gln Ser Pro Thr Gly  Ile Tyr Glu Lys Glu  Ile Asp Leu 
    1325                 1330                 1335             


Leu Arg  Val 
    1340     


<210>  880
<211>  1341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  880

Met Thr Gln Lys Val Ile Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asn Ser Val Gly Trp Ala Val Thr Asp Asn Glu Asn Lys Leu Leu His 
            20                  25                  30          


Ile Lys Gly His Asn Gly Ile Gly Val Arg Leu Phe Lys Glu Gly Gln 
        35                  40                  45              


Ser Ala Ala Glu Arg Cys Gly Phe Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Arg Leu Leu Asn Glu Ile Phe Ala Asp Lys 
65                  70                  75                  80  


Ile Ala Lys Val Asp Pro Ser Phe Phe Ala Arg Leu Lys Gln Ser Asn 
                85                  90                  95      


Val Ser Pro Lys Asp Pro Asn Lys Thr Met Phe Gly Asn Ile Leu Phe 
            100                 105                 110         


Asp Asn Gly Asn Leu Asn Asp Lys Ser Phe His Asp Glu Tyr Lys Thr 
        115                 120                 125             


Ile Tyr His Leu Arg Gln Ser Ile Ile Asp His Pro Glu Lys Lys Phe 
    130                 135                 140                 


Asp Ile Arg Leu Ile Tyr Leu Ala Met His His Ile Ile Lys Tyr Arg 
145                 150                 155                 160 


Gly His Phe Leu Asn Gln Ala Asn Val Gln Asp Phe Lys Gly Gly Lys 
                165                 170                 175     


Ile Asp Leu Glu Glu Ser Phe Lys Asp Leu Asn Ser Ile Phe Glu Asn 
            180                 185                 190         


Gln Gly Arg Asp Leu Arg Leu Val Thr Glu Asp Ala Asn Lys Tyr Val 
        195                 200                 205             


Lys Glu Leu Ser Asp Asn Ser Lys Thr Arg Asn Asp Arg Gln Lys Glu 
    210                 215                 220                 


Leu Ser Lys Val Leu Tyr Ile Pro Ile Asp Lys Asn Ser Asp Lys Asn 
225                 230                 235                 240 


Asn Lys Lys Val Thr Thr Glu Ile Ile Lys Ala Ile Leu Gly Met Lys 
                245                 250                 255     


Ala Lys Phe Asp Val Ile Phe Gly Ile Asp Ala Glu Asn Ala Lys Glu 
            260                 265                 270         


Trp Ser Leu Thr Phe Asn Ser Asp Asp Phe Asp Asp Lys Ile Glu Gly 
        275                 280                 285             


Leu Ser Ala Gln Met Thr Asp Glu Ala Gly Glu Ile Leu Leu Ile Leu 
    290                 295                 300                 


Lys Glu Leu Tyr Phe Ala Leu Asn Leu Ser Asp Ile Leu Asn Asn Ser 
305                 310                 315                 320 


Val Thr Asp Lys Asn Ala Lys Thr Leu Ser Glu Ala Met Ile Asn Arg 
                325                 330                 335     


Tyr Asp Asp His Lys Glu Gln Leu Thr Met Leu Lys Gln Val Ile Asp 
            340                 345                 350         


Asn Ser Asp Lys Lys His Ala Lys Ala Leu Lys Ala Ala Tyr Ser Ala 
        355                 360                 365             


Tyr Ile Asp Gly Glu Asn Asn Lys Lys Ile Ser Phe Glu Asp Leu Arg 
    370                 375                 380                 


Lys Arg Ile Gln Lys Asn Leu Asp Asn Ser Glu Thr Ser Lys Lys Ile 
385                 390                 395                 400 


Asn Asp Leu Leu Glu His Asp Tyr Phe Leu Pro Lys Gln Arg Ser Lys 
                405                 410                 415     


Glu Asn Gly Ala Ile Pro His Gln Leu Gln Gln Gln Glu Leu Asp Lys 
            420                 425                 430         


Ile Ile Glu Ser Gln Lys His Tyr Tyr Pro Phe Leu Ala Glu Leu Asn 
        435                 440                 445             


Pro Asn Glu Lys Arg Ser Arg Gln Ala Lys Tyr Lys Leu Asp Glu Leu 
    450                 455                 460                 


Val Ala Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Val Lys Ala Thr 
465                 470                 475                 480 


Glu Asn Asn Gln Ser Asn Asn Gly Lys Phe Ala Trp Met Ile Lys Asn 
                485                 490                 495     


Asp Asn Ile Ser Asp Lys Leu Lys Lys Glu Glu Ile Thr Pro Trp Asn 
            500                 505                 510         


Phe Asp Lys Lys Val Asp Arg Thr Glu Thr Ala Thr Lys Phe Ile Thr 
        515                 520                 525             


Arg Met Thr Thr Thr Asp Thr Tyr Leu Ile Gly Glu Pro Val Leu Pro 
    530                 535                 540                 


Lys Asn Ser Leu Leu Tyr Gln Lys Phe Val Val Leu Asn Glu Leu Asn 
545                 550                 555                 560 


Asn Leu Lys Ile Asn Lys Gln Pro Ile Thr Pro Glu Gln Lys Gln Tyr 
                565                 570                 575     


Val Tyr Glu His Val Met Lys Gln Thr Lys Arg Val Ser Leu Lys Lys 
            580                 585                 590         


Ile Ala Asp Ala Leu Val Ile Lys Gly Asp Tyr Pro Tyr Ala Pro Glu 
        595                 600                 605             


Ile Thr Gly Thr Thr Asp Gln Lys Asn Met Asn Asn Gly Leu Ser Thr 
    610                 615                 620                 


Tyr Ile Asp Leu Ala Lys Ile Phe Gly Asp Glu Leu Asp Asp Ile Asp 
625                 630                 635                 640 


Arg Lys Glu Asp Phe Glu Lys Ile Ile Glu Trp Ser Thr Ile Phe Glu 
                645                 650                 655     


Asp Gln His Ile Phe Ala Leu Lys Leu Lys Glu Ile Lys Trp Leu Thr 
            660                 665                 670         


Glu Asp Gln Arg Lys Gln Val Val Lys Leu Arg Tyr Gln Gly Trp Gly 
        675                 680                 685             


Lys Leu Ser Lys Lys Leu Leu Ala Asp Leu Val Asp Asp Asn Gly Gln 
    690                 695                 700                 


Arg Ile Ile Asp Leu Leu Trp Asp Ser Lys Gln Asp Phe Met Gln Ile 
705                 710                 715                 720 


Val Asn Gln Asn Ser Phe Lys Gln Ala Ile Ala Glu His Asn Gly Ala 
                725                 730                 735     


His Leu Glu Glu Lys Asp Leu Lys Asp Ile Ile Asn Asn Leu Tyr Thr 
            740                 745                 750         


Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Ile Leu Val Val Asp 
        755                 760                 765             


Asp Ile Ile Arg Ala Val Gly Tyr Glu Pro Val Asn Ile Met Met Glu 
    770                 775                 780                 


Phe Ala Arg Glu Asp Ser Lys Asp His Arg Leu Thr Asn Ser Arg Ser 
785                 790                 795                 800 


Arg Gln Leu Glu Lys Val Tyr Lys Asp Ile Thr Asn Ser Trp Phe Asp 
                805                 810                 815     


Asp Asp Asn Val Lys Asp Glu Leu Lys Asp Lys Ile Lys Asp Lys Ala 
            820                 825                 830         


Lys Phe Thr Asp Arg Leu Tyr Leu Tyr Phe Thr Gln Gly Gly Lys Asp 
        835                 840                 845             


Leu Tyr Thr Gly Glu Pro Leu Asp Ile Asp Asn Leu Ser Asn Tyr Gln 
    850                 855                 860                 


Ile Asp His Ile Leu Pro Gln Ser Phe Ile Leu Asp Asn Ser Leu Asp 
865                 870                 875                 880 


Asn Arg Val Leu Thr Ser Lys Ile Asn Asn Gln Ile Lys Lys Asp Asn 
                885                 890                 895     


Leu Pro Gly Glu Phe Phe Gly Ser Lys Met His Asn Phe Trp Lys His 
            900                 905                 910         


Leu Lys Glu Leu His Leu Ile Ser Asn Lys Lys Tyr Tyr Asn Leu Thr 
        915                 920                 925             


Leu Thr Pro Asn Ser Ile Ser Lys Phe Asn Asn Gln Gly Arg Phe Ile 
    930                 935                 940                 


Asn Arg Gln Leu Val Glu Thr Arg Gln Val Ile Lys Leu Ala Ala Glu 
945                 950                 955                 960 


Ile Leu His Asn Arg Tyr Ser Glu Glu Asn Gly Thr Asn Ile Val Thr 
                965                 970                 975     


Val Lys Ala Asn Leu Thr His His Met Arg Glu Lys Phe Asn Phe Tyr 
            980                 985                 990         


Lys Asn Arg Asn Val Asn Asp Tyr  His His Ala Phe Asp  Ala Tyr Leu 
        995                 1000                 1005             


Ala Ala  Phe Val Gly Asn Trp  Leu Leu Gln Gln Tyr  Pro Lys Leu 
    1010                 1015                 1020             


Lys Pro  Tyr Phe Val Tyr Gly  Asp Phe Ala Lys Thr  Glu Ile Asp 
    1025                 1030                 1035             


Asn Leu  Lys Ser Phe Asn Leu  Ile Tyr Lys Phe Glu  Gln Ala His 
    1040                 1045                 1050             


Gln Leu  Asn Glu Glu Asp Gly  Lys Ile Ala Lys Asp  Thr Asp Ala 
    1055                 1060                 1065             


Leu Leu  Gly Tyr Met Lys Asn  Val Tyr His Phe Lys  Lys Met Leu 
    1070                 1075                 1080             


Val Thr  Lys Glu Leu Glu Thr  Asn His Gly Asn Leu  Phe Lys Gln 
    1085                 1090                 1095             


Thr Leu  Tyr Pro Ser His Gly  Lys Met Lys Asn Leu  Ile Asn Pro 
    1100                 1105                 1110             


Lys Glu  Asn Arg Ser Ser Asp  Ile Tyr Gly Gly Tyr  Ser Ser Glu 
    1115                 1120                 1125             


Thr Gly  Ala Tyr Val Ser Leu  Ile Lys Val Phe Lys  Lys Asn Lys 
    1130                 1135                 1140             


Ile Glu  Asn Lys Ile Ile Lys  Ile Pro Leu Leu Tyr  Ile Asp Lys 
    1145                 1150                 1155             


Leu Asn  Arg Gln Ala Asn Ala  Lys Gln Phe Ile Arg  Asn Phe Ile 
    1160                 1165                 1170             


Ile Lys  Asp Asp Ser Asn Lys  Phe Asp Ile Leu Ser  Glu Lys Ile 
    1175                 1180                 1185             


Phe Leu  Asn Gln Leu Val Ile  Asp Gly Asp Glu Leu  Phe Thr Ile 
    1190                 1195                 1200             


Ser Gly  Ser Gly Asp Arg Gln  Asn Ala Gln Gln Leu  Ile Leu Ser 
    1205                 1210                 1215             


Asp Lys  Ser Leu Lys Ile Leu  Ser Lys Asn Ile Asn  Val Ile Ser 
    1220                 1225                 1230             


Lys Asn  Asp Leu Ile Ile Ile  Phe Asn Glu Ile Ile  Asp Asp Ile 
    1235                 1240                 1245             


Cys Lys  Tyr Met Lys Met Tyr  Glu Val Glu Asn Ser  Asp Glu Thr 
    1250                 1255                 1260             


Lys Ala  Lys Phe Glu Glu Ile  Asn Asp Lys Asp Arg  Met Leu Lys 
    1265                 1270                 1275             


Ile Ile  Asn Asp Leu Leu Leu  Gly Leu His Ala Asn  Ala Ser Arg 
    1280                 1285                 1290             


Ser Asn  Leu Ser Ser Ile Gly  Leu Ser Pro Ser Phe  Gly Arg Phe 
    1295                 1300                 1305             


Phe Ser  Gly Asn Ala Gly Ile  Lys Leu Ser Glu Asn  Ala Lys Phe 
    1310                 1315                 1320             


Ile Tyr  Gln Ser Pro Thr Gly  Leu Phe Ser Arg Lys  Val Lys Ile 
    1325                 1330                 1335             


Ser Asp  Leu 
    1340     


<210>  881
<211>  1343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  881

Met Thr Tyr Tyr Leu Gly Leu Asp Met Gly Thr Ser Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Thr Asp Glu Asn Tyr Asn Leu Ile His Arg His Arg Lys Asp 
            20                  25                  30          


Leu Trp Gly Ile Arg Glu Phe Glu Glu Ala Lys Thr Ala Val Glu Arg 
        35                  40                  45              


Arg Thr Gln Arg Ile Ser Arg Arg Arg Arg Gln Arg Glu Val Ala Arg 
    50                  55                  60                  


Ile Gly Leu Leu Lys Asp Tyr Phe His Asp Glu Ile Gln Lys Val Asp 
65                  70                  75                  80  


Pro Asn Phe Tyr Gln Arg Leu Asp Asn Ser Lys Tyr Phe Pro Glu Asp 
                85                  90                  95      


Lys Glu Glu Val Val Lys Gly Lys Asn Gly Ile Phe Ala Asp Glu Asn 
            100                 105                 110         


Tyr Thr Asp Arg Asp Tyr Phe Ala Glu Tyr Pro Thr Ile Phe His Leu 
        115                 120                 125             


Arg Arg Glu Leu Leu Asn Ser Lys Glu Pro His Asp Val Arg Leu Leu 
    130                 135                 140                 


Tyr Leu Ala Leu Leu Asn Met Tyr Lys His Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Thr Thr Leu Gly Thr Asp Gly Glu Asn Arg Lys Leu Ser Asp Val Tyr 
                165                 170                 175     


Arg Asp Phe Val Val Lys Thr Glu Glu Val Leu Gly Leu Thr Phe Pro 
            180                 185                 190         


Gln Glu Thr Asp Asn Gly Lys Met Gln Glu Ile Met Cys Ser Arg Glu 
        195                 200                 205             


Phe Ser Arg Lys Arg Lys Ala Glu Leu Leu Cys Ala Leu Leu Glu Ile 
    210                 215                 220                 


Asp Lys Lys Gln Lys Gln Gln Val Ala Leu Ile Glu Cys Val Leu Gly 
225                 230                 235                 240 


Leu Asp Lys Ser Val Lys Gln Met Tyr Gly Thr Ala Val Pro Asp Asp 
                245                 250                 255     


Phe Asp Lys Ser Val Lys Val Asn Phe Ser Asp Ser Ser Phe Glu Glu 
            260                 265                 270         


Lys Val Ser Glu Val Glu Glu Leu Leu Asp Glu Glu Lys Tyr Glu Leu 
        275                 280                 285             


Ile Leu Cys Met Lys Glu Leu Tyr Asp Thr Ala Val Leu Asn Gln Ile 
    290                 295                 300                 


Met Gly Asp Cys Asn Tyr Leu Ser Glu Ala Arg Val Gln Glu Tyr Glu 
305                 310                 315                 320 


Lys His Lys Glu Asp Leu Lys Leu Leu Lys Arg Thr Val Lys Lys Tyr 
                325                 330                 335     


Gly Thr Ala Glu Glu Tyr Gln Phe Leu Phe Arg Ser Ser Glu Lys Ala 
            340                 345                 350         


Ser Tyr Asn Ala Tyr Val His Ser Val Asn Ser Gly Glu Lys Ile Arg 
        355                 360                 365             


Arg Asn Met Asp Gly Arg Ser Arg Asp Asp Leu Tyr Thr Val Leu Lys 
    370                 375                 380                 


Lys Met Leu Lys Asn Tyr Pro Gln Glu Asp Ala Asp Cys Lys Tyr Ile 
385                 390                 395                 400 


Leu Glu Glu Ile Glu Lys Glu Gln Phe Leu Pro Lys Gln Leu Val Ser 
                405                 410                 415     


Asp Asn Gly Val Ile Pro Asn Gln Ile His Tyr Lys Glu Met Lys Lys 
            420                 425                 430         


Ile Leu Glu Asn Ala Glu Gln Tyr Leu His Phe Leu Arg Glu Lys Asp 
        435                 440                 445             


Glu Ser Gly Tyr Thr Val Ala Glu Arg Ile Leu Lys Leu Phe Ala Phe 
    450                 455                 460                 


Gln Ile Pro Tyr Tyr Ile Gly Pro Thr Ser Glu Lys Ser Gly Lys Asn 
465                 470                 475                 480 


Gly Gly Asn Gly Trp Val Val Arg Lys Glu Gln Gly Gln Val Leu Pro 
                485                 490                 495     


Trp Asn Ile Glu Glu Lys Ile Asp Met Lys Ala Thr Ser Glu Thr Phe 
            500                 505                 510         


Ile Ser Arg Met Val Arg Arg Cys Thr Tyr Leu Ser Asp Glu Met Val 
        515                 520                 525             


Leu Pro Lys Ala Ser Leu Glu Tyr Glu Ser Phe Cys Val Leu Asn Glu 
    530                 535                 540                 


Leu Asn Asn Leu Arg Ile Arg Gly Val Lys Leu Pro Ile Thr Val Lys 
545                 550                 555                 560 


Gln Asp Ile Tyr Lys Asp Leu Phe Glu Arg Gly Lys Arg Val Thr Arg 
                565                 570                 575     


Lys Gln Ile Val Asn Tyr Leu Lys Gly Val Gly Ile Leu Gln Glu Gly 
            580                 585                 590         


Glu Glu Asp Glu Leu Thr Gly Met Met Asp Thr Phe Asn Ser Ser Leu 
        595                 600                 605             


Ala Ser Tyr Gly Lys Phe Lys Ser Ile Leu Gly Glu Lys Ile Gln Glu 
    610                 615                 620                 


Asp Ser Cys Lys Arg Met Val Glu Gln Ile Ile Phe Trp Cys Thr Ile 
625                 630                 635                 640 


Tyr Gly Asp Ala Lys Lys Phe Leu Lys Glu Gln Leu Glu Glu Lys Tyr 
                645                 650                 655     


Gly Asp Val Leu Thr Arg Glu Gln Ile Lys Arg Ile Leu Gly Phe Lys 
            660                 665                 670         


Leu Lys Asp Trp Gly Asn Leu Ser Lys Lys Phe Leu Glu Leu Asn Gly 
        675                 680                 685             


Cys Asp Arg Asn Thr Gly Glu Val Met Ser Leu Ile Arg Met Leu Trp 
    690                 695                 700                 


Glu Ser Asp Asp Asn Leu Met Glu Leu Leu Lys Asp Ser Ser Tyr Thr 
705                 710                 715                 720 


Tyr Arg Ala Glu Leu Glu Glu Gln Gln Glu Lys Leu Thr Lys Thr Leu 
                725                 730                 735     


Thr Glu Ile Cys Pro Glu Asp Leu Glu Gly Tyr Tyr Phe Ser Ala Pro 
            740                 745                 750         


Val Lys Arg Met Ile Trp Gln Thr Leu Leu Ile Leu Lys Glu Val Glu 
        755                 760                 765             


Lys Val Met Gly Glu Pro Pro Lys Arg Val Phe Val Glu Met Thr Arg 
    770                 775                 780                 


Glu His Asp Glu Arg Arg Glu Met Lys Asp Ser Arg Gly Lys Lys Phe 
785                 790                 795                 800 


Gln Glu Leu Tyr Lys Asn Ile Lys Asp Glu Glu His Asn Trp Lys Ala 
                805                 810                 815     


Glu Ile Glu Asp Ala Glu Lys Asp Gly Arg Ile Arg Ser Lys Lys Met 
            820                 825                 830         


Tyr Leu Tyr Leu Thr Gln Arg Gly Arg Cys Met Tyr Thr Gly Asn Pro 
        835                 840                 845             


Ile Asp Leu Glu Glu Leu Phe Thr Thr Lys Tyr Asp Ile Asp His Ile 
    850                 855                 860                 


Tyr Pro Arg His Phe Val Lys Asp Asp Asn Leu Asp Asn Asn Leu Val 
865                 870                 875                 880 


Leu Val Glu Lys Glu Lys Asn Ala His Lys Ser Asp Gln Tyr Pro Leu 
                885                 890                 895     


Gly Asp Asp Ile Tyr Thr Gly Arg Leu Ser Trp Trp Lys Glu Leu His 
            900                 905                 910         


Arg Glu Lys Leu Ile Asn Asp Glu Lys Tyr Lys Arg Leu Thr Gly Arg 
        915                 920                 925             


Ala Pro Phe Ser Asp Glu Gln Lys Ala Gly Phe Ile Ala Arg Gln Leu 
    930                 935                 940                 


Val Glu Thr Ser Gln Gly Thr Lys Gly Val Ala Ser Ile Leu Lys Glu 
945                 950                 955                 960 


Val Leu Pro Glu Thr Thr Thr Ile Val Tyr Ala Lys Ala Arg Asn Val 
                965                 970                 975     


Ser Asp Phe Arg Asn Thr Asn Glu Leu Leu Lys Ala Arg Ser Val Asn 
            980                 985                 990         


Asp Phe His His Ala Gln Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn 
        995                 1000                 1005             


Val Tyr  Tyr Val Lys Phe Thr  Asn Asn Pro Leu Asn  Phe Val Arg 
    1010                 1015                 1020             


Ser Gly  Glu Gly Tyr Asn Leu  Asp Gly Val Phe Arg  Lys Asn Val 
    1025                 1030                 1035             


Ile Arg  Gly Gln Glu Asn Ala  Trp Ile Val Lys Pro  Glu Lys Gly 
    1040                 1045                 1050             


Glu Lys  Met Thr Leu Glu Thr  Val Lys Arg Val Met  Ala Lys Asn 
    1055                 1060                 1065             


Thr Pro  Leu Leu Thr Arg Leu  Ser Phe Glu Gly Arg  Gly Gly Leu 
    1070                 1075                 1080             


Ala Asn  Gln Thr Leu Tyr Ser  Ala Lys Glu Ala Ala  Gly Glu Gly 
    1085                 1090                 1095             


Tyr Val  Pro Leu Lys Ser Ser  Asp Ser Arg Leu Gln  Asp Val Thr 
    1100                 1105                 1110             


Lys Tyr  Gly Gly Phe Thr Ser  Val Thr Thr Ala Tyr  Phe Ile Leu 
    1115                 1120                 1125             


Val Glu  His Asp Met Lys Lys  Lys Arg Val Arg Thr  Ile Glu Ser 
    1130                 1135                 1140             


Val Pro  Ile Leu Trp Lys Glu  Arg Ile Glu Lys Glu  Ser Gly Gln 
    1145                 1150                 1155             


Leu Glu  Ile Tyr Cys Arg Glu  Ala Leu Gly Leu Val  Asn Pro Asp 
    1160                 1165                 1170             


Ile Arg  Val Arg Lys Ile Leu  Ile Gln Ser Leu Val  Lys Lys Asp 
    1175                 1180                 1185             


Gly Phe  Tyr Met His Leu Ser  Gly Lys Thr Gly Asn  Arg Phe Ile 
    1190                 1195                 1200             


Met Arg  Asn Ala Val Glu Leu  Cys Leu Lys Gln Glu  Trp Ile Asn 
    1205                 1210                 1215             


Tyr Ser  Lys Lys Leu Glu Lys  Glu Gly Cys Asp Glu  Ser Ile Thr 
    1220                 1225                 1230             


Lys Glu  Lys Asn Glu Glu Leu  Tyr Glu Ile Leu Lys  Glu Lys His 
    1235                 1240                 1245             


Ile Asn  Gly Ile Tyr Arg Lys  Arg Pro Asn Pro Met  Gly Glu Lys 
    1250                 1255                 1260             


Leu His  Ile Arg Gln Glu Thr  Phe Arg Met Leu Thr  Leu Glu Glu 
    1265                 1270                 1275             


Gln Lys  Lys Val Leu Leu Ser  Leu Leu Asn Leu Thr  Lys Ile Gly 
    1280                 1285                 1290             


Pro Val  Glu Ala Asn Leu Glu  Leu Ile Gly Glu Ser  Ala His Thr 
    1295                 1300                 1305             


Gly Lys  Met Leu Ile Ser Lys  Asn Ile Thr Glu Val  Lys Glu Phe 
    1310                 1315                 1320             


Tyr Leu  Ile His Gln Ser Val  Thr Gly Ile Tyr Glu  Lys Gln Val 
    1325                 1330                 1335             


Asn Leu  Leu Thr Val 
    1340             


<210>  882
<211>  1343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  882

Met Ala Thr Lys Lys Glu Tyr Asn Ile Gly Leu Asp Ile Gly Thr Thr 
1               5                   10                  15      


Ser Val Gly Trp Ser Val Val Glu Thr Asp Asn Gln Lys Val Met Arg 
            20                  25                  30          


Lys Gly Asn Lys Ala Leu Trp Gly Val Arg Leu Phe Glu Glu Ala Thr 
        35                  40                  45              


Thr Ala Glu Ser Arg Arg Met Gln Arg Ser Thr Arg Arg Arg Tyr Asp 
    50                  55                  60                  


Arg Arg Arg Glu Arg Ile Lys Leu Leu Gln Glu Glu Phe Ser Glu Glu 
65                  70                  75                  80  


Ile Asn Lys Val Asp Lys Asn Phe Phe Gln Lys Leu Lys Glu Ser Lys 
                85                  90                  95      


Tyr Val Glu Asn Asp Lys Ile Asn Lys Lys Ile Val Leu Thr Lys Glu 
            100                 105                 110         


Glu Lys Gln Glu Leu Lys Asp Tyr Gln Asn Lys Tyr Lys Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Asp Glu Leu Ile Asn Asn Pro Glu Lys Lys Asp Ile Arg 
    130                 135                 140                 


Leu Val Tyr Leu Ala Ile His His Ile Ile Lys Tyr Arg Gly Asn Phe 
145                 150                 155                 160 


Leu Tyr Gln Asn Ala Asn Phe Asn Ile Asp Asn Leu Asp Ile Lys Asp 
                165                 170                 175     


Lys Leu Asn Glu Leu Phe Ser Ile Leu Ser Asn Asn Ile Gln Glu Leu 
            180                 185                 190         


Glu Ile Pro Glu Asp Tyr Ile Asn Ile Ile Asp Phe Asp Glu Leu Glu 
        195                 200                 205             


Ala Asp Leu Leu Lys Asp Thr Lys Asn Asp Val Lys Ala Leu Leu Ile 
    210                 215                 220                 


Asp Asn Leu Thr Glu Leu Thr Asn Lys Ser Ser Ala Thr Glu Phe Gly 
225                 230                 235                 240 


Lys Leu Met Val Gly Asn Lys Gly Asn Ile Asn Lys Leu Leu Met Leu 
                245                 250                 255     


Glu Thr Asp Asn Lys Ile Glu Ile Ser Phe Ser Gly Thr Asp Tyr Asp 
            260                 265                 270         


Asp Lys Tyr Glu Glu Tyr Gln Glu Ala Leu Gly Glu Asn Ile Glu Ile 
        275                 280                 285             


Leu Asp Ile Leu Lys Gln Ile Tyr Asp Cys Ile Phe Leu Lys Lys Ile 
    290                 295                 300                 


Phe Lys Gly Asn Lys Asn Thr Ser Ile Ser Ser Leu Met Val Lys Ser 
305                 310                 315                 320 


Phe Glu Gln His Lys Lys Asp Leu Asn Phe Leu Lys Glu Leu Phe Lys 
                325                 330                 335     


Asp Asn Arg Glu Leu Tyr Asn Glu Ile Phe Arg Thr Lys Lys Asp Leu 
            340                 345                 350         


Cys Leu Tyr Glu Lys Tyr Ile Thr Asn Lys Ile Asp Cys Asp Glu Phe 
        355                 360                 365             


Arg Lys Glu Ile Asn Lys Leu Leu Glu Ser Leu Phe Ser Asp Glu Asn 
    370                 375                 380                 


Ile Lys Gln Asn Leu Leu Asp Glu Tyr Asn Leu Glu Val Lys Glu Arg 
385                 390                 395                 400 


Ile Glu Asn Gly Thr Phe Leu Pro Arg Ile Thr Thr Thr Asp Asn Gly 
                405                 410                 415     


Lys Tyr Pro Tyr Gln Leu Asn Lys Ser Glu Leu Ile Lys Ile Ile Glu 
            420                 425                 430         


Asn Gln Gly Lys Tyr Tyr Pro Phe Leu Leu Asn Lys Leu Asn Asp Asn 
        435                 440                 445             


Lys Thr Tyr Lys Ile Val Lys Leu Leu Glu Phe Arg Ile Pro Tyr Tyr 
    450                 455                 460                 


Val Gly Pro Leu Val Ser Glu Lys Arg Ser Glu Asn Ser Trp Leu Glu 
465                 470                 475                 480 


Arg Lys Ile Asn Asn Val Lys Ile Thr Pro Tyr Asn Phe Asp Asp Val 
                485                 490                 495     


Val Asp Lys Glu Lys Thr Ala Glu Lys Phe Ile Lys Arg Met Ile Ser 
            500                 505                 510         


His Cys Thr Tyr Leu Leu Asp Glu Tyr Ala Leu Ala Asn Asn Ser Ile 
        515                 520                 525             


Leu Tyr Ser Glu Phe Lys Val Met Asn Glu Leu Lys Gln Ile Arg Val 
    530                 535                 540                 


Asn Asp Arg Lys Leu Glu Leu Lys Phe Gln Gln Gln Ile Leu Glu Asp 
545                 550                 555                 560 


Phe Phe Lys Lys Thr Ser Gly Thr Ile Thr Glu Arg Lys Phe Lys Asn 
                565                 570                 575     


Tyr Leu Ile Ser Thr Gly Glu Tyr Ser Met Glu Gly Gly Asn Phe Arg 
            580                 585                 590         


Ile Thr Gly Tyr Ser Ala Asp Gly Lys Phe Ala Asn Asn Met Gln Ser 
        595                 600                 605             


Tyr Val Asp Phe Phe Gly Glu Asn Gly Ile Phe Ala Asp Thr Asp Tyr 
    610                 615                 620                 


Asn Leu Glu Asn Ala Glu Glu Ile Ile Glu Trp Ile Thr Ile Phe Glu 
625                 630                 635                 640 


Asp Lys Asp Ile Leu Glu Lys Lys Val Arg Asp Asn Tyr Ser Lys Leu 
                645                 650                 655     


Asn Glu Asn Gln Ile Lys Lys Val Leu Ala Lys Lys Tyr Ser Gly Trp 
            660                 665                 670         


Gly Ser Leu Ser Lys Lys Leu Leu Lys Thr Lys Tyr Tyr Lys Asp Lys 
        675                 680                 685             


Ala Thr Glu Leu Tyr Lys Ser Ile Leu Asp Leu Met Tyr Glu Thr Asp 
    690                 695                 700                 


Lys Asn Phe Met Gln Ile Ile Asn Asp Asp Lys Tyr Asn Phe Gln Glu 
705                 710                 715                 720 


Met Ile Lys Lys Phe Asn Asn Lys Glu Glu Asn Lys Lys Leu Asn Tyr 
                725                 730                 735     


Ser Ile Val Glu Glu Leu Ala Thr Ser Pro Ala Thr Lys Arg Gly Ile 
            740                 745                 750         


Tyr Gln Ala Leu Lys Val Val Glu Glu Leu Val Asp Tyr Ile Gly Tyr 
        755                 760                 765             


Glu Pro Lys Asn Ile Ser Ile Glu Met Ala Arg Ser Glu Glu Glu Lys 
    770                 775                 780                 


Val Arg Lys Asp Ser Arg Lys Asp Tyr Ile Asn Asn Leu Tyr Lys Gly 
785                 790                 795                 800 


Cys Lys Asp Ser Ile Glu Asn Tyr Lys Lys Leu Lys His Glu Leu Asp 
                805                 810                 815     


Ser His Glu Ile Thr Ser Gln Arg Leu Phe Leu Tyr Phe Ile Gln Glu 
            820                 825                 830         


Gly Lys Cys Leu Tyr Thr Gly Thr Pro Leu Asn Ile Glu Asp Ile Glu 
        835                 840                 845             


Asn Gln Ser Leu Tyr Glu Ile Asp His Ile Ile Pro Arg Ser Leu Ile 
    850                 855                 860                 


Lys Asp Asp Ser Ile Asp Asn Lys Ala Leu Val Leu Lys Glu Cys Asn 
865                 870                 875                 880 


Gln Asp Lys Lys Ala Ser Tyr Val Leu Pro Ser Lys Phe Arg Asn Ser 
                885                 890                 895     


Lys Gln Lys Thr Trp Trp Lys His Leu Lys Asp Asn Gly Leu Met Ser 
            900                 905                 910         


Ala Lys Lys Phe His Asn Leu Ile Arg Glu Lys Tyr Asn Glu Glu Asp 
        915                 920                 925             


Ile Asn Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
    930                 935                 940                 


Lys His Val Ala Asn Ile Leu Asn Asn Leu Tyr Lys Asp Thr Asn Val 
945                 950                 955                 960 


Ile Tyr Leu Lys Ala Asp Leu Ser His Asn Tyr Arg Glu Lys Tyr Asp 
                965                 970                 975     


Leu Phe Lys Phe Arg Asp Ile Asn Asp Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Ala Ala Val Leu Gly Glu  Tyr Lys Glu Arg Phe  Met Lys Arg 
        995                 1000                 1005             


Lys Ile  Asn Phe Asp Met Val  Lys Glu Leu Asn Ser  Lys Ile Val 
    1010                 1015                 1020             


Glu Met  Asp Glu Gly Lys Arg  Arg Asn Leu Arg Phe  Gly Tyr Val 
    1025                 1030                 1035             


Ile Asn  Ser Leu Asp Glu Asn  Leu Asn Asp Ile Val  Leu Lys Ile 
    1040                 1045                 1050             


Ser Glu  Asn Leu Val Asp Asn  Glu Thr Gly Glu Val  Leu Phe Asp 
    1055                 1060                 1065             


Ala His  Glu Phe Asn Lys Arg  Val Glu Asp Thr Leu  Tyr Arg Asn 
    1070                 1075                 1080             


Asp Ile  Leu Ile Ser Arg Lys  Thr Glu Ile Arg Thr  Gly Gln Phe 
    1085                 1090                 1095             


Tyr Lys  Glu Thr Ile Tyr Lys  Lys Gly Lys Gly Thr  Ile Pro Ile 
    1100                 1105                 1110             


Lys Phe  Gly Met Pro Thr Asp  Ile Tyr Gly Gly Tyr  Ser Asn Thr 
    1115                 1120                 1125             


Asn Ala  Arg Lys Leu Met Leu  Ile Glu Tyr Asn Glu  Ser Lys Met 
    1130                 1135                 1140             


Lys Ile  Ile Gly Leu Pro Met  Ala Leu Ala Val Arg  Asn Asn Asp 
    1145                 1150                 1155             


Ala Glu  Ile Asn Asp Tyr Ile  Lys Thr Gln Ile Asn  Leu Lys Lys 
    1160                 1165                 1170             


Asn Ser  Thr Phe Lys Ile Lys  Lys Cys Ser Ile Pro  Phe Glu Thr 
    1175                 1180                 1185             


Glu Ile  Ile Tyr Asn Asn Gln  Asn Val Phe Ile Lys  Gly Tyr Ser 
    1190                 1195                 1200             


Ile Gly  His Lys Asn Cys Glu  Ile Ser Asn Ala Ile  Gln Leu Lys 
    1205                 1210                 1215             


Ile Ala  Lys Asp Lys Met Lys  His Trp Lys Tyr Ala  Leu Gln Tyr 
    1220                 1225                 1230             


Leu Leu  Asn Asp Lys Lys Gln  Tyr Ile Asp Phe Ala  Glu Lys Tyr 
    1235                 1240                 1245             


Val Asn  Glu Ile Tyr Asp Phe  Leu Ile Asn Leu Asp  Leu Phe Pro 
    1250                 1255                 1260             


Leu Phe  Asn Lys Glu Leu Asp  Lys Ile Lys Asn Lys  Val Asn Phe 
    1265                 1270                 1275             


Glu Asp  Leu Ala Leu Asp Gln  Gln Lys Lys Ile Thr  Lys Glu Leu 
    1280                 1285                 1290             


Phe Lys  Met Leu His Cys Asn  Ser Val Asn Ala Asn  Leu Ser Glu 
    1295                 1300                 1305             


Phe Gly  Leu Gly Asp Arg Met  Gly Arg Leu Ser Gly  Tyr Asn Ile 
    1310                 1315                 1320             


Thr Asp  Gly Ile Ile Ile Ser  Lys Ser Val Thr Gly  Ile Arg Glu 
    1325                 1330                 1335             


Ser Arg  Tyr Glu Phe 
    1340             


<210>  883
<211>  1344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  883

Met Ser Gln Asn Asn Asn Lys Ile Tyr Asn Ile Gly Leu Asp Ile Gly 
1               5                   10                  15      


Asp Ala Ser Val Gly Trp Ala Val Val Asp Glu His Tyr Asn Leu Leu 
            20                  25                  30          


Lys Arg His Gly Lys His Met Trp Gly Ser Arg Leu Phe Thr Gln Ala 
        35                  40                  45              


Asn Thr Ala Val Glu Arg Arg Ser Ser Arg Ser Thr Arg Arg Arg Tyr 
    50                  55                  60                  


Asn Lys Arg Arg Glu Arg Ile Arg Leu Leu Arg Glu Ile Met Glu Asp 
65                  70                  75                  80  


Met Val Leu Asp Val Asp Pro Thr Phe Phe Ile Arg Leu Ala Asn Val 
                85                  90                  95      


Ser Phe Leu Asp Gln Glu Asp Lys Lys Asp Tyr Leu Lys Glu Asn Tyr 
            100                 105                 110         


His Ser Asn Tyr Asn Leu Phe Ile Asp Lys Asp Phe Asn Asp Lys Thr 
        115                 120                 125             


Tyr Tyr Asp Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Cys 
    130                 135                 140                 


Glu Ser Lys Glu Lys Glu Asp Pro Arg Leu Ile Tyr Leu Ala Leu His 
145                 150                 155                 160 


His Ile Val Lys Tyr Arg Gly Asn Phe Leu Tyr Glu Gly Gln Lys Phe 
                165                 170                 175     


Ser Met Asp Val Ser Asn Ile Glu Asp Lys Met Ile Asp Val Leu Arg 
            180                 185                 190         


Gln Phe Asn Glu Ile Asn Leu Phe Glu Tyr Val Glu Asp Arg Lys Lys 
        195                 200                 205             


Ile Asp Glu Val Leu Asn Val Leu Lys Glu Pro Leu Ser Lys Lys His 
    210                 215                 220                 


Lys Ala Glu Lys Ala Phe Ala Leu Phe Asp Thr Thr Lys Asp Asn Lys 
225                 230                 235                 240 


Ala Ala Tyr Lys Glu Leu Cys Ala Ala Leu Ala Gly Asn Lys Phe Asn 
                245                 250                 255     


Val Thr Lys Met Leu Lys Glu Ala Glu Leu His Asp Glu Asp Glu Lys 
            260                 265                 270         


Asp Ile Ser Phe Lys Phe Ser Asp Ala Thr Phe Asp Asp Ala Phe Val 
        275                 280                 285             


Glu Lys Gln Pro Leu Leu Gly Asp Cys Val Glu Phe Ile Asp Leu Leu 
    290                 295                 300                 


His Asp Ile Tyr Ser Trp Val Glu Leu Gln Asn Ile Leu Gly Ser Ala 
305                 310                 315                 320 


His Thr Ser Glu Pro Ser Ile Ser Ala Ala Met Ile Gln Arg Tyr Glu 
                325                 330                 335     


Asp His Lys Asn Asp Leu Lys Leu Leu Lys Asp Val Ile Arg Lys Tyr 
            340                 345                 350         


Leu Pro Lys Lys Tyr Phe Glu Val Phe Arg Asp Glu Lys Ser Lys Lys 
        355                 360                 365             


Asn Asn Tyr Cys Asn Tyr Ile Asn His Pro Ser Lys Thr Pro Val Asp 
    370                 375                 380                 


Glu Phe Tyr Lys Tyr Ile Lys Lys Leu Ile Glu Lys Ile Asp Asp Pro 
385                 390                 395                 400 


Asp Val Lys Thr Ile Leu Asn Lys Ile Glu Leu Glu Ser Phe Met Leu 
                405                 410                 415     


Lys Gln Asn Ser Arg Thr Asn Gly Ala Val Pro Tyr Gln Met Gln Leu 
            420                 425                 430         


Asp Glu Leu Asn Lys Ile Leu Glu Asn Gln Ser Val Tyr Tyr Ser Asp 
        435                 440                 445             


Leu Lys Asp Asn Glu Asp Lys Ile Arg Ser Ile Leu Thr Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Phe Gly Pro Leu Asn Ile Thr Lys Asp Arg Gln Phe Asp 
465                 470                 475                 480 


Trp Ile Ile Lys Lys Glu Gly Lys Glu Asn Glu Arg Ile Leu Pro Trp 
                485                 490                 495     


Asn Ala Asn Glu Ile Val Asp Val Asp Lys Thr Ala Asp Glu Phe Ile 
            500                 505                 510         


Lys Arg Met Arg Asn Phe Cys Thr Tyr Phe Pro Asp Glu Pro Val Met 
        515                 520                 525             


Ala Lys Asn Ser Leu Thr Val Ser Lys Tyr Glu Val Leu Asn Glu Ile 
    530                 535                 540                 


Asn Lys Leu Arg Ile Asn Asp His Leu Ile Lys Arg Asp Met Lys Asp 
545                 550                 555                 560 


Lys Met Leu His Thr Leu Phe Met Asp His Lys Ser Ile Ser Ala Asn 
                565                 570                 575     


Ala Met Lys Lys Trp Leu Val Lys Asn Gln Tyr Phe Ser Asn Thr Asp 
            580                 585                 590         


Asp Ile Lys Ile Glu Gly Phe Gln Lys Glu Asn Ala Cys Ser Thr Ser 
        595                 600                 605             


Leu Thr Pro Trp Ile Asp Phe Thr Lys Ile Phe Gly Lys Ile Asn Glu 
    610                 615                 620                 


Ser Asn Tyr Asp Phe Ile Glu Lys Ile Ile Tyr Asp Val Thr Val Phe 
625                 630                 635                 640 


Glu Asp Lys Lys Ile Leu Arg Arg Arg Leu Lys Lys Glu Tyr Asp Leu 
                645                 650                 655     


Asp Glu Glu Lys Ile Lys Lys Ile Leu Lys Leu Lys Tyr Ser Gly Trp 
            660                 665                 670         


Ser Arg Leu Ser Lys Lys Leu Leu Ser Gly Ile Lys Thr Lys Tyr Lys 
        675                 680                 685             


Asp Ser Thr Arg Thr Pro Glu Thr Val Leu Glu Val Met Glu Arg Thr 
    690                 695                 700                 


Asn Met Asn Leu Met Gln Val Ile Asn Asp Glu Lys Leu Gly Phe Lys 
705                 710                 715                 720 


Lys Thr Ile Asp Asp Ala Asn Ser Thr Ser Val Ser Gly Lys Phe Ser 
                725                 730                 735     


Tyr Ala Glu Val Gln Glu Leu Ala Gly Ser Pro Ala Ile Lys Arg Gly 
            740                 745                 750         


Ile Trp Gln Ala Leu Leu Ile Val Asp Glu Ile Lys Lys Ile Met Lys 
        755                 760                 765             


His Glu Pro Ala His Val Tyr Ile Glu Phe Ala Arg Asn Glu Asp Glu 
    770                 775                 780                 


Lys Glu Arg Lys Asp Ser Phe Val Asn Gln Met Leu Lys Leu Tyr Lys 
785                 790                 795                 800 


Asp Tyr Asp Phe Glu Asp Glu Thr Glu Lys Glu Ala Asn Lys His Leu 
                805                 810                 815     


Lys Gly Glu Asp Ala Lys Ser Lys Ile Arg Ser Glu Arg Leu Lys Leu 
            820                 825                 830         


Tyr Tyr Thr Gln Met Gly Lys Cys Met Tyr Thr Gly Lys Ser Leu Asp 
        835                 840                 845             


Ile Asp Arg Leu Asp Thr Tyr Gln Val Asp His Ile Val Pro Gln Ser 
    850                 855                 860                 


Leu Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Val Leu Ser Ser 
865                 870                 875                 880 


Glu Asn Gln Arg Lys Leu Asp Asp Leu Val Ile Pro Ser Ser Ile Arg 
                885                 890                 895     


Asn Lys Met Tyr Gly Phe Trp Glu Lys Leu Phe Asn Asn Lys Ile Ile 
            900                 905                 910         


Ser Pro Lys Lys Phe Tyr Ser Leu Ile Lys Thr Glu Phe Asn Glu Lys 
        915                 920                 925             


Asp Gln Glu Arg Phe Ile Asn Arg Gln Ile Val Glu Thr Arg Gln Ile 
    930                 935                 940                 


Thr Lys His Val Ala Gln Ile Ile Asp Asn His Tyr Glu Asn Thr Lys 
945                 950                 955                 960 


Val Val Thr Val Arg Ala Asp Leu Ser His Gln Phe Arg Glu Arg Tyr 
                965                 970                 975     


His Ile Tyr Lys Asn Arg Asp Ile Asn Asp Phe His His Ala His Asp 
            980                 985                 990         


Ala Tyr Ile Ala Thr Ile Leu Gly  Thr Tyr Ile Gly His  Arg Phe Glu 
        995                 1000                 1005             


Ser Leu  Asp Ala Lys Tyr Ile  Tyr Gly Glu Tyr Lys  Arg Ile Phe 
    1010                 1015                 1020             


Arg Asn  Gln Lys Asn Lys Gly  Lys Glu Met Lys Lys  Asn Asn Asp 
    1025                 1030                 1035             


Gly Phe  Ile Leu Asn Ser Met  Arg Asn Ile Tyr Ala  Asp Lys Asp 
    1040                 1045                 1050             


Thr Gly  Glu Ile Val Trp Asp  Pro Asn Tyr Ile Asp  Arg Ile Lys 
    1055                 1060                 1065             


Lys Cys  Phe Tyr Tyr Lys Asp  Cys Phe Val Thr Lys  Lys Leu Glu 
    1070                 1075                 1080             


Glu Asn  Asn Gly Thr Phe Phe  Asn Val Thr Val Leu  Pro Asn Asp 
    1085                 1090                 1095             


Thr Asn  Ser Asp Lys Asp Asn  Thr Leu Ala Thr Val  Pro Val Asn 
    1100                 1105                 1110             


Lys Tyr  Arg Ser Asn Val Asn  Lys Tyr Gly Gly Phe  Ser Gly Val 
    1115                 1120                 1125             


Asn Ser  Phe Ile Val Ala Ile  Lys Gly Lys Lys Lys  Lys Gly Lys 
    1130                 1135                 1140             


Lys Val  Ile Glu Val Asn Lys  Leu Thr Gly Ile Pro  Leu Met Tyr 
    1145                 1150                 1155             


Lys Asn  Ala Asp Glu Glu Ile  Lys Ile Asn Tyr Leu  Lys Gln Ala 
    1160                 1165                 1170             


Glu Asp  Leu Glu Glu Val Gln  Ile Gly Lys Glu Ile  Leu Lys Asn 
    1175                 1180                 1185             


Gln Leu  Ile Glu Lys Asp Gly  Gly Leu Tyr Tyr Ile  Val Ala Pro 
    1190                 1195                 1200             


Thr Glu  Ile Ile Asn Ala Lys  Gln Leu Ile Leu Asn  Glu Ser Gln 
    1205                 1210                 1215             


Thr Lys  Leu Val Cys Glu Ile  Tyr Lys Ala Met Lys  Tyr Lys Asn 
    1220                 1225                 1230             


Tyr Asp  Asn Leu Asp Ser Glu  Lys Ile Ile Asp Leu  Tyr Arg Leu 
    1235                 1240                 1245             


Leu Ile  Asn Lys Met Glu Leu  Tyr Tyr Pro Glu Tyr  Arg Lys Gln 
    1250                 1255                 1260             


Leu Val  Lys Lys Phe Glu Asp  Arg Tyr Glu Gln Leu  Lys Val Ile 
    1265                 1270                 1275             


Ser Ile  Glu Glu Lys Cys Asn  Ile Ile Lys Gln Ile  Leu Ala Thr 
    1280                 1285                 1290             


Leu His  Cys Asn Ser Ser Ile  Gly Lys Ile Met Tyr  Ser Asp Phe 
    1295                 1300                 1305             


Lys Ile  Ser Thr Thr Ile Gly  Arg Leu Asn Gly Arg  Thr Ile Ser 
    1310                 1315                 1320             


Leu Asp  Asp Ile Ser Phe Ile  Ala Glu Ser Pro Thr  Gly Met Tyr 
    1325                 1330                 1335             


Ser Lys  Lys Tyr Lys Leu 
    1340                 


<210>  884
<211>  1344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  884

Met Lys Lys Thr Asn Pro Tyr Ile Leu Ser Leu Asp Ile Gly Thr Ala 
1               5                   10                  15      


Ser Ile Gly Tyr Ala Cys Met Asp Leu Asp Phe Asn Ile Leu Lys Tyr 
            20                  25                  30          


His Asp Lys Asp Ala Ile Gly Val Leu Thr Phe Glu Gly Ala Asp Thr 
        35                  40                  45              


Ala Ala Glu Arg Arg Gly Phe Arg Thr Ser Arg Arg Arg Lys Asn Arg 
    50                  55                  60                  


Arg Ile Lys Arg Leu Gly Leu Leu Gln Glu Val Leu Ala Pro Leu Val 
65                  70                  75                  80  


Lys Asn Pro Asn Tyr Tyr Gln Leu Leu Asn Leu His Lys Trp Lys Asn 
                85                  90                  95      


Ser Asn Glu Asp Phe Lys His Arg Ser Leu Ser Glu Val Leu Lys Ile 
            100                 105                 110         


Leu Gly Lys Asn Pro Lys Thr Tyr Pro Thr Ile Tyr His Leu Gln Gln 
        115                 120                 125             


Asp Ile Gln Asn His Pro Glu Lys Lys Phe Glu Pro Glu Leu Ile Phe 
    130                 135                 140                 


Leu Ala Leu Tyr His Leu Val Lys Tyr Arg Gly His Phe Leu Phe Asn 
145                 150                 155                 160 


Asn Leu Ser Phe Asp Lys Gly Lys Thr Val Asp Asn Glu Ala Gln Leu 
                165                 170                 175     


Ser Glu Leu Leu Glu Gln Tyr Gln Ser Ile Asn Pro Tyr Glu Asp Ser 
            180                 185                 190         


Ile Ser Asn Lys Asp Ile Asn Val Ile Val Asp Thr Leu Lys Asp Asp 
        195                 200                 205             


Gln Leu Thr Arg Asn Asp Lys Ser Lys Glu Leu Lys Arg Asn Val Ser 
    210                 215                 220                 


Lys Thr His Ala Glu Leu Phe Lys Met Leu Val Gly Leu Lys Phe Lys 
225                 230                 235                 240 


Gln Ser Ile Leu Phe Glu Asn Met Glu Asn Ala Glu Asp Ile Lys Glu 
                245                 250                 255     


Ser Ala Thr Gly Ser Asp Ala Ile Ser Leu Ser Asp Asp Tyr Tyr Glu 
            260                 265                 270         


Asn Leu Ser Glu Phe Leu Thr Asp Glu Gln Leu Asn Phe Ile Glu Ser 
        275                 280                 285             


Ala Asn Thr Phe Phe Met Gly Ile Met Leu Asp Arg Ile Leu Lys Gly 
    290                 295                 300                 


Ala Thr Ser Ile Ser Ala Ala Lys Val Arg Asp Tyr Lys Ile Phe Lys 
305                 310                 315                 320 


Asn Gln Leu Lys Ile Ile Lys Asp Ile Val Tyr Gln Asn Lys Tyr Asp 
                325                 330                 335     


Lys Tyr His Glu Phe Phe Val Thr Ser Lys Glu Asp Met Arg Ala Tyr 
            340                 345                 350         


Gln Glu Asn Pro Asp Ala Lys Thr Phe Gly Lys Leu Ser Lys Phe Asp 
        355                 360                 365             


Gln Tyr Leu Ile His Pro Lys Asp Gln Tyr Ala Lys Leu Met Lys Asp 
    370                 375                 380                 


Leu Lys Gly Ile Val Pro Lys Lys Tyr Thr Glu Val Leu Asn Leu Ile 
385                 390                 395                 400 


Asp Glu Gln Lys Phe Leu Val Val Gln Asn Thr Thr Asp Asn Ala Ser 
                405                 410                 415     


Ile Pro Tyr Gln Asn Asn Val Phe Glu Ala Glu Asn Ile Leu Arg Asn 
            420                 425                 430         


Gln Gln Lys Tyr Tyr Pro Glu Ile Thr Glu Glu Met Ile Gly Lys Val 
        435                 440                 445             


Ile Asn Leu Ile Ser Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Thr 
    450                 455                 460                 


Asn Lys Asn Gly Gln Ser Asp Phe Ala Trp Met Ile Arg Lys Ser Asp 
465                 470                 475                 480 


Glu Gln Thr Leu Pro Trp Asn Phe Asp Lys Val Ile Asp Lys Ser Lys 
                485                 490                 495     


Ser Ala Glu Lys Phe Ile Arg Arg Met Thr Ser Lys Cys Thr Tyr Leu 
            500                 505                 510         


Leu Glu Glu Asp Val Leu Pro Lys His Ser Leu Leu Tyr Gln Glu Met 
        515                 520                 525             


Glu Val Leu Asn Glu Leu Asn Asn Val Gln Leu Arg Gly Glu Asn Glu 
    530                 535                 540                 


Pro Lys His Arg Ile His Arg Leu Asp Pro Lys Val Lys Gln Phe Ile 
545                 550                 555                 560 


Tyr His Asn Leu Phe Gln Lys Lys Lys Asn Val Thr His Lys Asp Leu 
                565                 570                 575     


Ile Thr Leu Leu Gln Asn Ser Glu His Arg Asn Thr Leu Asn Lys Thr 
            580                 585                 590         


Ser Gly Gln Lys Leu Lys Val Phe Gly Thr Gln Asn Asp Ser Lys Phe 
        595                 600                 605             


Ala Ser Lys Leu Ser Thr Tyr Asn Asp Met Lys Ala Ile Phe Gly Ser 
    610                 615                 620                 


Val Glu Asn Lys Arg Gln Met Ile Glu Glu Leu Val Leu Trp Ile Thr 
625                 630                 635                 640 


Ile Phe Glu Asp Lys Ala Ile Leu Lys Gln Lys Ile Gly Glu Lys Tyr 
                645                 650                 655     


Pro Asn Ile Thr Glu Lys Gln Leu Asn Gln Ile Val Lys Leu Asn Tyr 
            660                 665                 670         


Ser Gly Trp Gly Arg Leu Ser Glu Lys Leu Leu Thr Lys Glu Val Asn 
        675                 680                 685             


Gly Arg Ser Leu Ile Asp Leu Met Tyr Ser Thr Glu Asn Asn Phe Met 
    690                 695                 700                 


Glu Ile Ile Thr Asp Lys Thr Leu Gly Phe Asp Cys Phe Ile Lys Asn 
705                 710                 715                 720 


Ser Asn Lys Val Lys Asn Ala Lys Ile Ser Tyr Lys Asp Val Ala Glu 
                725                 730                 735     


Leu Arg Thr Ser Pro Ala Leu Lys Lys Gly Ile Trp Asn Ala Ile Lys 
            740                 745                 750         


Met Val Arg Glu Ile Ser Asp Leu Phe Gly Ala Pro Glu Lys Ile Ile 
        755                 760                 765             


Ile Glu Phe Ala Thr Glu Asp Gln Val Lys Gly Lys Arg Gln Lys Ser 
    770                 775                 780                 


Arg Ser Glu Leu Trp Asp Asp Leu Val Lys Lys Asn Asn Leu Lys Arg 
785                 790                 795                 800 


Asn Lys Lys Phe Lys Gly Leu Phe Glu Glu Leu Lys Ala Tyr Pro Asp 
                805                 810                 815     


Leu Asp Phe Ser Asn Pro Lys Leu Trp Leu Tyr Ile His Gln Asn Gly 
            820                 825                 830         


Lys Cys Met Tyr Thr Lys Lys Pro Ile Asp Leu Glu Arg Leu Met Ser 
        835                 840                 845             


Asp Thr Asn Asn Gln Leu Tyr Glu Ile Asp His Ile Leu Pro Arg Thr 
    850                 855                 860                 


Phe Val Lys Asp Asp Ser Ile Asn Asn Lys Val Leu Val Ile Gln Ser 
865                 870                 875                 880 


Ala Asn Gln Lys Lys Ser Gly Asp Lys Met Pro Leu Glu Ile Phe Asp 
                885                 890                 895     


Asn His Val Leu Val Pro Phe Trp Lys Met Leu Tyr Glu Asn Asp Leu 
            900                 905                 910         


Ile Ser Ser Ser Lys Leu Ser Lys Leu Met Lys Lys Glu Phe Asn Asp 
        915                 920                 925             


Leu Asp Lys Glu Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Ile Ile Ser His Val Ser Asp Phe Leu Thr Glu Glu Tyr Pro Ser Thr 
945                 950                 955                 960 


Thr Val Val Pro Met Lys Ala Asn Val Val Ser Glu Leu Arg Arg Lys 
                965                 970                 975     


Phe Asp Ile Pro Lys Ile Arg Gln Leu Asn Asp Trp His His Ala Val 
            980                 985                 990         


Asp Ala Tyr Leu Asn Gly Phe Val  Tyr Gln Ala Ala Arg  Val Phe Tyr 
        995                 1000                 1005             


Pro Lys  Val Asn Leu Phe Glu  Phe Asn Phe Lys Trp  Glu Lys Val 
    1010                 1015                 1020             


Arg Glu  Lys Trp Arg Gln Leu  Gly Glu Phe Thr Glu  Asn Asn Lys 
    1025                 1030                 1035             


Lys Arg  Glu Phe Phe Leu Phe  Lys Arg Met Glu Asn  Tyr Val Asn 
    1040                 1045                 1050             


Glu His  Gly Glu Gly Met Ile  Ser Lys Leu Ile Phe  Asp Leu Asp 
    1055                 1060                 1065             


Asn Phe  Lys Phe Asn Tyr Ser  Arg Lys Val Glu Ser  Thr Glu Ser 
    1070                 1075                 1080             


Ala Phe  Tyr Lys Gln Ser Ala  Phe Ser Pro Lys Asn  Thr Thr Ala 
    1085                 1090                 1095             


Gln Tyr  Asp Ser Asp Lys Asn  Lys Thr Ala Ile Tyr  Lys Glu Met 
    1100                 1105                 1110             


Lys Asn  Lys Arg Gly Ile Val  Val Ser Tyr Thr Tyr  Val Lys Lys 
    1115                 1120                 1125             


Asp Gln  Glu Leu Ile Gly Tyr  Gly Val Phe Asn Glu  Thr Val Phe 
    1130                 1135                 1140             


Glu Tyr  Val Asn Asn Ala Asp  Val Asp Met Asn Thr  Arg Ala Leu 
    1145                 1150                 1155             


Lys Ile  Val Arg Lys Ala Phe  Pro Lys Leu Asp Ile  Lys Lys Ala 
    1160                 1165                 1170             


Gln Tyr  Leu Phe Ser Ile Asn  Lys Gly Asp Ala Leu  Leu Val Asn 
    1175                 1180                 1185             


Gly His  Pro Phe Leu Phe Val  Ser Ala Lys Glu Arg  Ile Asn Gly 
    1190                 1195                 1200             


Lys Gln  Leu Glu Phe Asp Ile  Asn Met Gln Arg Arg  Leu Lys Lys 
    1205                 1210                 1215             


Ala Leu  Arg Pro Tyr Asn Lys  Met Thr Glu Gln Glu  Lys Val Gly 
    1220                 1225                 1230             


Val Tyr  Asn Gln Phe Ala Glu  Lys Val Val Gln Glu  Tyr Glu Asn 
    1235                 1240                 1245             


Ser Leu  Gly Ala Lys Thr Arg  Asn Glu Lys Leu Glu  Leu Ile Arg 
    1250                 1255                 1260             


Asn Gln  Phe Asn Glu Ser Asp  Lys Ser Asn Glu Ala  Leu Ile Gln 
    1265                 1270                 1275             


Cys Ile  Leu Glu Val Leu Lys  Met Thr Lys Val Ser  Ala Glu Arg 
    1280                 1285                 1290             


Ser Val  Lys Leu Lys Ile Arg  Trp Gly Ser Gln Thr  Pro Asn Thr 
    1295                 1300                 1305             


Leu Ile  Asp Ile Asn Asn Lys  Ala Lys Ile Met Tyr  Asn Ser Ile 
    1310                 1315                 1320             


Thr Gly  Leu Lys Gln Thr Arg  Leu Lys Ser Ile Tyr  Lys Leu Ala 
    1325                 1330                 1335             


Glu Ser  Lys Asn Glu Leu 
    1340                 


<210>  885
<211>  1344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  885

Met Asn Lys Asn Tyr Tyr Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Gln Glu Tyr Asn Leu Ile Lys Leu Lys Gly 
            20                  25                  30          


Thr His Ala Trp Gly Ala Arg Ile Phe Gln Glu Ala Lys Asp Lys Lys 
        35                  40                  45              


Gly Thr Arg Ala Ile Arg Ser Ser Arg Arg Arg Met Asp Arg Arg Lys 
    50                  55                  60                  


Tyr Arg Ile His Leu Leu Asn Gln Leu Phe Ala Gln Glu Ile Gln Ala 
65                  70                  75                  80  


Ile Asp Ser Thr Phe Phe Ile Arg Leu Ala Tyr Ser Ala Tyr Trp Glu 
                85                  90                  95      


Val Asp Lys Lys Asp Lys Gly Ile Gly Arg Asn Ile Leu Phe Lys Thr 
            100                 105                 110         


Lys Gln Glu Glu Ser Gln Phe Tyr Lys Lys Tyr Pro Thr Ile Trp His 
        115                 120                 125             


Leu Arg Lys Ser Leu Leu Glu Asn Gln Pro Asp Ala Phe Ser Asp Leu 
    130                 135                 140                 


Arg Tyr Val Tyr Leu Ala Phe His His Ile Met Lys Tyr Arg Gly Asn 
145                 150                 155                 160 


Phe Leu His Glu Gly Glu Ile Lys Thr Asn Glu Leu Gln Val Gln Gln 
                165                 170                 175     


Ile Glu Gln Leu Asn Gln Phe Leu Ile Asn Lys Tyr Lys Glu Ile Tyr 
            180                 185                 190         


Asp Asn Asp Val Glu Glu Arg Thr Phe Ile Ala Asn His Gln Ile Asp 
        195                 200                 205             


Ala Leu Lys Glu Ile Leu Leu Asn Lys Asn Thr Asn Lys Thr Thr Lys 
    210                 215                 220                 


Glu Lys Glu Ile Lys Lys Leu Phe Asp Tyr Ala Asp Cys Glu Ser Leu 
225                 230                 235                 240 


Glu Pro Tyr Ile Ser Cys Phe Ser Lys Leu Ala Val Gly Gly Lys Phe 
                245                 250                 255     


Asp Leu Ser Lys Leu Gly Tyr Glu Gly Asn Ile Gln Phe Ser Asp Pro 
            260                 265                 270         


Ser Phe Glu Glu Lys Ala Ser Ile Leu Gln Asp Asp Phe Arg Leu Ile 
        275                 280                 285             


Ala Ile Ala Lys Glu Met Tyr Asp Phe Val Ile Leu Asn Asp Leu Leu 
    290                 295                 300                 


Lys Asp Gln Pro Asn Leu Ser Thr Ala Met Val His Val Tyr Glu Thr 
305                 310                 315                 320 


His Arg Gln Asp Leu Lys Lys Leu Lys Asp Ile Ile Ile Asp Ile Asp 
                325                 330                 335     


Ala Asn Lys Gly Leu Gln Asn Asn Asp Arg Trp Tyr Phe Lys Leu Phe 
            340                 345                 350         


Lys Asp Lys Asn Ala Pro Lys Asn Tyr Ser Ala Phe Ile His Lys Asn 
        355                 360                 365             


Ser Asn Gln Lys Arg Pro Ser Ile Asp Glu Phe Asn Asp Glu Ile Lys 
    370                 375                 380                 


Lys Ile Leu Ile Glu His Arg Asn Asp Val Ser Leu Glu Lys Gln Asp 
385                 390                 395                 400 


Asp Leu Lys Met Leu Phe Glu Lys Val Glu Asn Lys Thr Leu Leu Lys 
                405                 410                 415     


Thr Ile Ala Ile Gln Ser Thr Ser Val Ile Pro His Gln Leu His Leu 
            420                 425                 430         


Asp Glu Leu Glu Lys Ile Ile Asp Asn Ala Ser Thr Lys Tyr Pro Phe 
        435                 440                 445             


Leu Ser Asn Ile Arg Asp Lys Leu Val Leu Leu Phe Thr Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Tyr Gly Pro Leu Asn Asp Lys Ser Pro Tyr Ser Asn Val 
465                 470                 475                 480 


Glu Arg Lys Gln Phe Ala Thr Val Thr Pro Trp Asn Phe Asp Glu Ile 
                485                 490                 495     


Phe Asp Glu Asn Lys Thr Arg Glu Lys Phe Met Lys Lys Leu Thr Asn 
            500                 505                 510         


Asn Cys Thr Tyr Leu Ser Gly Glu Thr Val Met Pro Lys Lys Ala Leu 
        515                 520                 525             


Ala Tyr Glu Asp Tyr Leu Ile Tyr Asp Arg Leu Asn Val Leu Leu Val 
    530                 535                 540                 


Asn Gly Glu Tyr Leu Ser Leu Gln Glu Lys Glu Lys Ile Tyr His Tyr 
545                 550                 555                 560 


Leu Ile Ser Arg Pro Lys Thr Thr Leu Asp Gln Leu Lys Lys Glu Leu 
                565                 570                 575     


Ala Lys Trp His Asn Gln Ser Pro Ile Asp Ile Ile Phe Ser Asn Ile 
            580                 585                 590         


His Glu Glu Ile Pro Phe Glu Ala Ser Ser His Ala His Leu Met Lys 
        595                 600                 605             


Ser Phe Asp Leu Glu Lys Gln Lys Glu Glu Leu Glu Arg Tyr Ile Thr 
    610                 615                 620                 


Leu Ala Thr Ile Tyr Ala Asp Asp Lys Lys Ser Leu Lys Glu Leu Leu 
625                 630                 635                 640 


Lys Asn Glu Ser Leu Leu Asp Glu Asn Gln Lys Asn Val Ile Leu Thr 
                645                 650                 655     


Leu Pro Thr Lys Lys Trp Ala Pro Leu Ser Tyr Lys Leu Leu Gln Asn 
            660                 665                 670         


Ile Val Tyr Thr Asp Asp Tyr Gly Val Val His Ser Ile Leu Asp Ile 
        675                 680                 685             


Met Lys Glu Thr Asn Gln Asn Phe Gln Met Val Leu His Asn Glu Gln 
    690                 695                 700                 


Tyr His Phe Lys Glu Leu Ile Glu Gln Glu Asn Gln Lys Phe Thr Gln 
705                 710                 715                 720 


Glu Lys Thr Ile Glu Asp Leu Ile Glu Glu Lys Leu Glu Ser Ile Pro 
                725                 730                 735     


Ser Leu Thr Gln Arg Ser Ile Arg Gln Thr Leu Leu Ile Ile Asp Asp 
            740                 745                 750         


Ile Ile Lys Ala Ser His Gln Lys Val Pro Glu Lys Ile Phe Ile Glu 
        755                 760                 765             


Val Thr Arg Asn Asn Asp Asp Lys Arg Lys Gly Gln Asp Gly Asn Gln 
    770                 775                 780                 


Leu Ala Arg Asp Lys Glu Leu Asn Arg Phe Leu Thr Asn Leu Leu Lys 
785                 790                 795                 800 


Asp Glu Lys Ala Asn Gln Phe Cys Asp Val Ser Gln Leu Leu Lys Asp 
                805                 810                 815     


Leu Glu Glu Val Asp Lys Val Lys Leu Lys Gly Lys His Ile Tyr Leu 
            820                 825                 830         


Tyr Phe Lys Gln Leu Gly Ile Asp Leu Tyr Thr Gly Lys Lys Ile Val 
        835                 840                 845             


Leu Asn Gln Leu Leu Thr Ser Thr Lys Tyr Asp Thr Asp His Ile Ile 
    850                 855                 860                 


Pro Gln Ser Leu Ile Lys Asp Asp Ser Leu Asp Asn Leu Val Leu Val 
865                 870                 875                 880 


Glu Arg Glu Val Asn Leu Lys Val Lys Gly Asn His Tyr Pro Val Pro 
                885                 890                 895     


Ser Arg Ile Gln Asp Glu Gln Lys Lys Leu Trp His Phe Leu Leu Gln 
            900                 905                 910         


Lys Lys Ala Ile Ser Glu Lys Lys Tyr Gly Asn Leu Thr Arg Thr Ser 
        915                 920                 925             


Lys Ile Lys Leu Glu Glu Val Glu Asp Phe Val Ala Arg Gln Ile Asn 
    930                 935                 940                 


Val Ile Asn Tyr Ser Asn Val Ile Ile Arg Asp Ile Leu Lys Leu Lys 
945                 950                 955                 960 


Tyr Pro His Thr Asp Ile Ile Phe Ser Lys Ser Gln Tyr Pro Ser Phe 
                965                 970                 975     


Ile Arg Lys His Leu Lys Ile Val Lys Asn Arg Asp Val Asn Asp Ala 
            980                 985                 990         


His His Ala Val Asp Ala Tyr Leu  Asn Val Val Ser Gly  Asn Leu Leu 
        995                 1000                 1005             


Ser Thr  Thr Phe Ser Lys Ala  Tyr Ile Arg Lys Lys  Tyr Ile Gln 
    1010                 1015                 1020             


Ile Gln  Asp Gly Glu Lys Glu  Thr Phe Asn Met Glu  Thr Ile Leu 
    1025                 1030                 1035             


Glu Asn  Tyr Phe Lys Asn Asn  Glu Asn Gln Leu Lys  Ile Lys Thr 
    1040                 1045                 1050             


Asn Cys  Leu Arg Arg Asp Ala  Leu Ile Thr Phe Lys  Val Asp Tyr 
    1055                 1060                 1065             


Phe Ser  Gly Glu Phe Tyr Gly  Gln Thr Arg Leu Pro  Lys Gly Asn 
    1070                 1075                 1080             


Glu Asn  Leu Ile Pro Ile Thr  Leu Asn Glu Asp Asn  Pro Met Ser 
    1085                 1090                 1095             


Asp Thr  Ser Lys Tyr Gly Gly  Tyr Thr Asn Leu Ala  Gln Ala Tyr 
    1100                 1105                 1110             


Leu Phe  Ala Val Ser Tyr Gln  Glu Asp Gly Glu Met  Lys Lys Ala 
    1115                 1120                 1125             


Ile Leu  Arg Val Pro Tyr Leu  Tyr Asp Thr Leu Tyr  Lys Asn Asp 
    1130                 1135                 1140             


Ser Asp  Lys Leu Leu Ser Lys  Val Leu Asp Asn Pro  Lys Ala Thr 
    1145                 1150                 1155             


Asn Ile  Gln Ile Leu Arg Lys  Ile His Leu Asn Gln  Lys Ile Arg 
    1160                 1165                 1170             


Phe Asn  Asp Gly Ile Tyr Leu  Leu Tyr Thr Asn Glu  Lys Asp Arg 
    1175                 1180                 1185             


Asn Lys  Tyr Lys Met Ala Tyr  Gln Asn Tyr Ile Asp  Asn Asp Tyr 
    1190                 1195                 1200             


Leu Tyr  Tyr Leu His Trp Ala  Asn Lys His Leu Glu  Glu Leu Leu 
    1205                 1210                 1215             


Asp Asn  Gln Glu Lys Gln Thr  Phe Ile Tyr Asn Ser  Lys Asn Asp 
    1220                 1225                 1230             


Gln Phe  Glu Val Ser Lys Ala  Lys Asn Lys Glu Ile  Phe Gln Tyr 
    1235                 1240                 1245             


Leu Leu  Gln Lys Thr Lys Ala  Lys Val Tyr Asp Thr  Cys Asn Tyr 
    1250                 1255                 1260             


Ile Val  Lys Ala Arg Glu Thr  Asn Ile Glu Ile Phe  Glu Gln Leu 
    1265                 1270                 1275             


Thr Leu  Lys Glu Gln Ile Thr  Met Ile Asn Gln Trp  Ile Lys Met 
    1280                 1285                 1290             


Met Ser  Arg Asp Cys Glu Lys  Val Lys Phe Asp Ala  Arg Phe Thr 
    1295                 1300                 1305             


Asn Phe  Ser Asn Cys Leu Leu  Arg Leu Ser Asn Asn  Ile Asn Pro 
    1310                 1315                 1320             


Lys Asp  Ser Ile Thr Ile Ile  Tyr Glu Ser Pro Thr  Gly Leu Phe 
    1325                 1330                 1335             


Lys His  Glu Val Lys Ile 
    1340                 


<210>  886
<211>  1344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  886

Met Lys Lys Thr Asn Pro Tyr Ile Leu Ser Leu Asp Ile Gly Thr Ala 
1               5                   10                  15      


Ser Ile Gly Tyr Ala Cys Met Asp Leu Asp Phe Asn Ile Leu Lys Tyr 
            20                  25                  30          


His Asp Lys Asp Ala Ile Gly Val Leu Thr Phe Glu Gly Ala Asp Thr 
        35                  40                  45              


Ala Ala Glu Arg Arg Gly Phe Arg Thr Ser Arg Arg Arg Lys Asn Arg 
    50                  55                  60                  


Arg Ile Lys Arg Leu Gly Leu Leu Gln Glu Val Leu Ala Pro Leu Val 
65                  70                  75                  80  


Lys Asn Pro Asn Tyr Tyr Gln Leu Leu Asn Leu His Lys Trp Lys Asn 
                85                  90                  95      


Ser Asn Glu Asp Phe Lys His Arg Ser Leu Ser Glu Val Leu Lys Thr 
            100                 105                 110         


Leu Gly Lys Asn Pro Lys Thr Tyr Pro Thr Ile Tyr His Leu Gln Gln 
        115                 120                 125             


Asp Ile Gln Asn His Pro Glu Lys Lys Phe Glu Pro Glu Leu Ile Phe 
    130                 135                 140                 


Leu Ala Leu Tyr His Leu Val Lys Tyr Arg Gly His Phe Leu Phe Asn 
145                 150                 155                 160 


Asn Leu Ser Phe Asp Lys Gly Lys Thr Val Asp Asn Glu Ala Gln Leu 
                165                 170                 175     


Ser Glu Leu Leu Glu Gln Tyr Gln Ser Ile Asn Pro Tyr Glu Gly Ser 
            180                 185                 190         


Ile Phe Asn Lys Asp Ile Lys Val Ile Val Asp Thr Leu Lys Asp Asp 
        195                 200                 205             


Gln Leu Thr Arg Asn Asp Lys Ser Lys Glu Leu Lys Arg Asn Val Ser 
    210                 215                 220                 


Lys Thr His Ala Glu Leu Phe Lys Met Leu Val Gly Leu Lys Phe Lys 
225                 230                 235                 240 


Gln Ser Ile Leu Phe Glu Asn Met Glu Asn Val Glu Asp Ile Lys Glu 
                245                 250                 255     


Ser Ala Thr Gly Ser Asp Ala Ile Ser Leu Ser Asp Asp Tyr Tyr Glu 
            260                 265                 270         


Asn Leu Ser Glu Phe Leu Thr Asp Glu Gln Leu Asn Phe Ile Glu Ser 
        275                 280                 285             


Ala Asn Thr Phe Phe Met Gly Ile Met Leu Asp Arg Ile Leu Lys Gly 
    290                 295                 300                 


Ala Thr Ser Ile Ser Ala Ala Lys Val Arg Asp Tyr Lys Ile Phe Lys 
305                 310                 315                 320 


Asn Gln Leu Lys Leu Ile Lys Asp Ile Val Tyr Gln Asn Lys Tyr Asp 
                325                 330                 335     


Lys Tyr His Glu Phe Phe Val Thr Ser Lys Glu Asp Met Arg Ala Tyr 
            340                 345                 350         


Gln Glu Asn Pro Asp Ala Lys Thr Phe Gly Lys Leu Ser Lys Phe Asp 
        355                 360                 365             


Gln Tyr Leu Ile His Pro Lys Asp Gln Tyr Ala Lys Leu Met Lys Asp 
    370                 375                 380                 


Leu Lys Gly Ile Val Pro Lys Lys Tyr Thr Glu Val Leu Asn Leu Ile 
385                 390                 395                 400 


Asp Glu Gln Lys Phe Leu Val Val Gln Asn Thr Thr Asp Asn Ala Ser 
                405                 410                 415     


Ile Pro Tyr Gln Asn Asn Val Phe Glu Ala Glu Asn Ile Leu Arg Asn 
            420                 425                 430         


Gln Gln Lys Tyr Tyr Pro Glu Ile Thr Glu Glu Met Ile Gly Lys Val 
        435                 440                 445             


Ile Asn Leu Ile Ser Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Thr 
    450                 455                 460                 


Asn Lys Asn Gly Gln Ser Asp Phe Ala Trp Met Ile Arg Lys Ser Asp 
465                 470                 475                 480 


Glu Gln Ala Leu Pro Trp Asn Phe Asp Lys Val Ile Asp Lys Ser Lys 
                485                 490                 495     


Ser Ala Glu Lys Phe Ile Arg Arg Met Thr Ser Lys Cys Thr Tyr Leu 
            500                 505                 510         


Leu Glu Glu Asp Val Leu Pro Lys His Ser Leu Leu Tyr Gln Glu Met 
        515                 520                 525             


Glu Val Leu Asn Glu Leu Asn Asn Val Gln Leu Arg Gly Glu Asn Glu 
    530                 535                 540                 


Pro Lys His Arg Ile His Arg Leu Asp Pro Lys Val Lys Gln Phe Ile 
545                 550                 555                 560 


Tyr His Asn Leu Phe Gln Lys Lys Lys Asn Val Thr His Lys Asp Leu 
                565                 570                 575     


Ile Thr Leu Leu Gln Asn Ser Glu His Arg Asn Thr Leu Asn Lys Thr 
            580                 585                 590         


Ser Gly Gln Lys Leu Lys Val Phe Gly Thr Gln Asn Asp Ser Lys Phe 
        595                 600                 605             


Ala Ser Lys Leu Ser Thr Tyr Asn Asp Met Lys Ala Ile Phe Gly Ser 
    610                 615                 620                 


Val Glu Asn Lys Arg Gln Met Ile Glu Glu Leu Val Leu Trp Val Thr 
625                 630                 635                 640 


Ile Phe Glu Asp Lys Ala Ile Leu Lys Gln Lys Ile Gly Glu Lys Tyr 
                645                 650                 655     


Pro Asn Ile Thr Glu Lys Gln Leu Asn Gln Ile Val Lys Leu Asn Tyr 
            660                 665                 670         


Ser Gly Trp Gly Arg Leu Ser Glu Lys Leu Leu Thr Lys Glu Val Asn 
        675                 680                 685             


Gly Arg Ser Leu Ile Asp Leu Met Tyr Ser Thr Glu Asn Asn Phe Met 
    690                 695                 700                 


Glu Ile Ile Thr Asp Lys Thr Leu Gly Phe Asp Cys Phe Ile Lys Asn 
705                 710                 715                 720 


Ser Asn Lys Val Lys Asn Ala Lys Ile Ser Tyr Lys Asp Val Ala Glu 
                725                 730                 735     


Leu Arg Thr Ser Pro Ala Leu Lys Lys Gly Ile Trp Asn Ala Ile Lys 
            740                 745                 750         


Met Val Arg Glu Ile Ser Asn Leu Phe Gly Ala Pro Glu Lys Ile Ile 
        755                 760                 765             


Ile Glu Phe Ala Thr Glu Asp Gln Val Lys Gly Lys Arg Gln Lys Ser 
    770                 775                 780                 


Arg Ser Glu Leu Trp Asp Asp Leu Val Lys Lys Asn Asn Leu Lys Arg 
785                 790                 795                 800 


Asn Lys Glu Phe Lys Gly Leu Phe Glu Glu Ile Lys Ala Tyr Pro Asp 
                805                 810                 815     


Leu Asp Phe Ser Asn Pro Lys Leu Trp Leu Tyr Ile His Gln Asn Gly 
            820                 825                 830         


Lys Cys Met Tyr Thr Lys Lys Pro Ile Asp Leu Glu Arg Leu Leu Ser 
        835                 840                 845             


Asp Thr Asn Asn Gln Leu Tyr Glu Ile Asp His Ile Leu Pro Arg Thr 
    850                 855                 860                 


Phe Val Lys Asp Asp Ser Ile Asn Asn Lys Val Leu Val Ile Gln Ser 
865                 870                 875                 880 


Ala Asn Gln Lys Lys Ser Gly Asp Lys Met Pro Leu Glu Ile Phe Asp 
                885                 890                 895     


Asn His Val Leu Val Pro Phe Trp Lys Met Leu Tyr Glu Asn Asp Leu 
            900                 905                 910         


Ile Ser Ser Ser Lys Leu Ser Lys Leu Met Lys Lys Glu Phe Asn Asp 
        915                 920                 925             


Leu Asp Lys Glu Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Ile Ile Ser His Val Ser Asp Phe Leu Thr Glu Glu Tyr Pro Ser Thr 
945                 950                 955                 960 


Thr Val Val Pro Met Lys Ala Asn Val Val Ser Glu Leu Arg Arg Lys 
                965                 970                 975     


Phe Asp Ile Pro Lys Ile Arg Gln Leu Asn Asp Trp His His Ala Val 
            980                 985                 990         


Asp Ala Tyr Leu Asn Gly Phe Val  Tyr Gln Ala Ala Arg  Val Phe Tyr 
        995                 1000                 1005             


Pro Lys  Val Asn Leu Phe Glu  Phe Asn Phe Lys Trp  Glu Lys Val 
    1010                 1015                 1020             


Arg Glu  Lys Trp Arg Gln Leu  Gly Glu Phe Thr Glu  Asn Asn Lys 
    1025                 1030                 1035             


Lys Arg  Glu Phe Phe Leu Phe  Lys Arg Met Glu Asn  Tyr Val Asn 
    1040                 1045                 1050             


Glu His  Gly Glu Gly Met Ile  Ser Lys Leu Ile Phe  Asp Leu Asp 
    1055                 1060                 1065             


Asn Phe  Lys Phe Asn Tyr Ser  Arg Lys Val Glu Ser  Thr Glu Ser 
    1070                 1075                 1080             


Ala Phe  Tyr Lys Gln Ser Ala  Phe Ser Pro Lys Asn  Thr Thr Ala 
    1085                 1090                 1095             


Gln Tyr  Asp Ser Asp Lys Asn  Lys Thr Ala Ile Tyr  Lys Glu Met 
    1100                 1105                 1110             


Lys Asn  Lys Arg Gly Ile Val  Val Ser Tyr Thr Tyr  Val Lys Lys 
    1115                 1120                 1125             


Asp Gln  Glu Leu Ile Gly Tyr  Gly Val Phe Asn Glu  Thr Val Phe 
    1130                 1135                 1140             


Glu Tyr  Val Asn Asn Ala Asp  Val Asp Met Asn Thr  Arg Ala Leu 
    1145                 1150                 1155             


Lys Ile  Val Arg Lys Ala Phe  Pro Lys Leu Asp Ile  Lys Lys Val 
    1160                 1165                 1170             


Gln Tyr  Leu Phe Ser Ile Asn  Lys Gly Asp Thr Leu  Leu Val Asn 
    1175                 1180                 1185             


Gly His  Pro Phe Leu Phe Val  Ser Ala Tyr Glu Arg  Ile Asn Gly 
    1190                 1195                 1200             


Lys Gln  Leu Glu Phe Asp Ile  Ala Met Gln Arg Lys  Ile Lys Gln 
    1205                 1210                 1215             


Ala Leu  Ser Pro Tyr Asn Asn  Met Thr Glu Gln Glu  Lys Val Glu 
    1220                 1225                 1230             


Phe Tyr  Asn Gln Phe Ala Glu  Lys Val Ile Glu Glu  Phe Arg Asn 
    1235                 1240                 1245             


Ser Ile  Gly Leu Gln Ser Arg  Asp Arg Lys Val Asn  Leu Ile Lys 
    1250                 1255                 1260             


Asn Leu  Phe Asn Asn Thr Glu  Lys Ser Asn Asp Ser  Met Thr Gln 
    1265                 1270                 1275             


Ser Ile  Leu Glu Val Leu Lys  Met Thr Lys Val Ser  Ala Glu Arg 
    1280                 1285                 1290             


Ser Lys  Glu Leu Lys Ile Arg  Trp Asn Gly Gln Thr  Pro Asn Thr 
    1295                 1300                 1305             


Phe Ile  Asp Thr Lys Asn Asn  Ala Glu Ile Gln Tyr  Lys Ser Ile 
    1310                 1315                 1320             


Thr Gly  Leu Lys Thr Thr Lys  Pro Lys Ser Ile Phe  Lys Leu Ala 
    1325                 1330                 1335             


Glu Ser  Lys Asn Glu Leu 
    1340                 


<210>  887
<211>  1345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  887

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Ser His Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Gly Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Thr Glu Asp Lys Arg 
            100                 105                 110         


Gly Glu Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Lys Tyr 
        115                 120                 125             


His Glu Asn Phe Pro Thr Ile Tyr His Leu Arg Gln Tyr Leu Ala Asp 
    130                 135                 140                 


Asn Pro Glu Lys Thr Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Gly Gly His Phe Leu Ile Glu Gly Lys Phe Asp Thr 
                165                 170                 175     


Arg Asn Asn Asp Val Gln Arg Leu Phe Gln Glu Phe Leu Ala Val Tyr 
            180                 185                 190         


Asp Asn Thr Phe Glu Asn Ser Ser Leu Gln Glu Gln Asn Val Gln Val 
        195                 200                 205             


Glu Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 


Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Arg Phe Ala Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     


Glu Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Ile Tyr Glu 
            260                 265                 270         


Glu Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu 
        275                 280                 285             


Leu Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Val Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys 
                325                 330                 335     


Gln Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         


Asp Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             


Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu 
    370                 375                 380                 


Gly Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Asp Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp 
    450                 455                 460                 


Leu Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 


Ile Val Asp Lys Glu Ser Ser Val Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Asn Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Lys Thr Glu Gln Gly Lys Thr Ala Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr Lys 
545                 550                 555                 560 


Asp Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     


Val Asp Leu Thr Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ala Ser 
            580                 585                 590         


Tyr Gly Thr Tyr His Asp Leu Arg Lys Ile Leu Asp Lys Asp Phe Leu 
        595                 600                 605             


Asp Asn Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Lys Asn Tyr 
625                 630                 635                 640 


Ser Asp Leu Leu Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
        675                 680                 685             


Asn Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser 
    690                 695                 700                 


Phe Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn 
705                 710                 715                 720 


Leu Asn Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met 
            740                 745                 750         


Gly His Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu 
    770                 775                 780                 


Thr Asp Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr 
            820                 825                 830         


Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             


Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asp Val Pro Ser Lys Asn Val Val Arg Lys Met Lys 
865                 870                 875                 880 


Ser Tyr Trp Ser Lys Leu Leu Ser Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe His Thr Glu Thr Asp 
    930                 935                 940                 


Glu Asn Asn Lys Lys Ile Arg Gln Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 


Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Ile Gly Lys Ala Leu Leu Gly Val  Tyr Pro Gln Leu Glu  Pro Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Pro His  Phe His Gly His Lys  Glu Asn Lys 
    1010                 1015                 1020             


Ala Thr  Ala Lys Lys Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe 
    1025                 1030                 1035             


Lys Lys  Asp Asp Val Arg Thr  Asp Lys Asn Gly Glu  Ile Ile Trp 
    1040                 1045                 1050             


Lys Lys  Asp Glu His Ile Ser  Asn Ile Lys Lys Val  Leu Ser Tyr 
    1055                 1060                 1065             


Pro Gln  Val Asn Ile Val Lys  Lys Val Glu Glu Gln  Thr Gly Gly 
    1070                 1075                 1080             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly Asn Ser  Asp Lys Leu 
    1085                 1090                 1095             


Ile Pro  Arg Lys Thr Lys Lys  Phe Tyr Trp Asp Thr  Lys Lys Tyr 
    1100                 1105                 1110             


Gly Gly  Phe Asp Ser Pro Ile  Val Ala Tyr Ser Ile  Leu Val Ile 
    1115                 1120                 1125             


Ala Asp  Ile Glu Lys Gly Lys  Ser Lys Lys Leu Lys  Thr Val Lys 
    1130                 1135                 1140             


Ala Leu  Val Gly Val Thr Ile  Met Glu Lys Met Thr  Phe Glu Arg 
    1145                 1150                 1155             


Asp Pro  Val Ala Phe Leu Glu  Arg Lys Gly Tyr Arg  Asn Val Gln 
    1160                 1165                 1170             


Glu Glu  Asn Ile Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Lys Leu 
    1175                 1180                 1185             


Glu Asn  Gly Arg Lys Arg Leu  Leu Ala Ser Ala Arg  Glu Leu Gln 
    1190                 1195                 1200             


Lys Gly  Asn Glu Ile Val Leu  Pro Asn His Leu Gly  Thr Leu Leu 
    1205                 1210                 1215             


Tyr His  Ala Lys Asn Ile His  Lys Val Asp Glu Pro  Lys His Leu 
    1220                 1225                 1230             


Asp Tyr  Val Asp Lys His Lys  Asp Glu Phe Lys Glu  Leu Leu Asp 
    1235                 1240                 1245             


Val Val  Ser Asn Phe Ser Lys  Lys Tyr Thr Leu Ala  Glu Gly Asn 
    1250                 1255                 1260             


Leu Glu  Lys Ile Lys Glu Leu  Tyr Ala Gln Asn Asn  Gly Glu Asp 
    1265                 1270                 1275             


Leu Lys  Glu Leu Ala Ser Ser  Phe Ile Asn Leu Leu  Thr Phe Thr 
    1280                 1285                 1290             


Ala Ile  Gly Ala Pro Ala Thr  Phe Lys Phe Phe Asp  Lys Asn Ile 
    1295                 1300                 1305             


Asp Arg  Lys Arg Tyr Thr Ser  Thr Thr Glu Ile Leu  Asn Ala Thr 
    1310                 1315                 1320             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1325                 1330                 1335             


Leu Ser  Lys Leu Gly Gly Asp  
    1340                 1345 


<210>  888
<211>  1345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  888

Met Glu Asn Tyr Asn Ile Gly Leu Asp Ile Gly Thr Thr Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Val Asn Asp Ala Thr Asn Lys Val Met Arg Lys Gly Gly 
            20                  25                  30          


Lys Ala Leu Trp Gly Val Arg Leu Phe Asp Pro Ala Val Thr Ala Glu 
        35                  40                  45              


Asp Arg Arg Ala Lys Arg Gly Thr Arg Arg Arg Tyr Asp Arg Arg Arg 
    50                  55                  60                  


Glu Arg Ile Lys Leu Leu Gln Asp Glu Phe Lys Asp Glu Ile Asn Lys 
65                  70                  75                  80  


Val Asp Ser Asn Phe Phe Leu Lys Leu Glu Glu Ser Lys Tyr Thr Ser 
                85                  90                  95      


Lys Asp Ile Lys Asn Lys Thr Val Leu Ile Ser Met Glu Glu Glu Lys 
            100                 105                 110         


Ala Ile Lys Lys Tyr Asn Gln Glu Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Gln Lys Leu Met Asn Ser Asn Glu Gln Met Asp Ile Arg Leu Val Tyr 
    130                 135                 140                 


Leu Ala Ile His His Ile Ile Lys Tyr Arg Gly Asn Phe Ile Tyr Glu 
145                 150                 155                 160 


Gly Asn Ser Phe Ser Val Asn Asn Leu Asn Ile Asp Asn Lys Leu Lys 
                165                 170                 175     


Glu Ile Phe Leu Ser Leu Ser Glu Asp Val Asp Glu Leu Asp Ile Ser 
            180                 185                 190         


Glu Glu Cys Ile Asn Asn Ile Asn Tyr Lys Gln Leu Ala Asn Ile Phe 
        195                 200                 205             


Asn Glu Gln Ser Lys Asn Asp Leu Lys Val Lys Leu Thr Ser Glu Leu 
    210                 215                 220                 


Thr Ser Tyr Ser Leu Asn Lys Asn Phe Ile Ser Glu Phe Cys Lys Leu 
225                 230                 235                 240 


Ile Ile Gly Asn Lys Phe Ser Ala Lys Lys Leu Phe Asn Leu Glu Thr 
                245                 250                 255     


Asp Asn Asp Val Ser Leu Ser Phe Asp Gly Thr Asp Phe Glu Asp Lys 
            260                 265                 270         


Ala Gly Asp Leu Glu Ser Leu Leu Asn Asp Tyr Leu Glu Val Leu Glu 
        275                 280                 285             


Gln Leu Lys Glu Leu Tyr Asp Met Val Phe Leu Lys Lys Ile Phe Gly 
    290                 295                 300                 


Gly Ser Lys Glu Thr Thr Ile Ser Gly Leu Met Val Glu Arg Tyr Asn 
305                 310                 315                 320 


Gln His Lys Lys Asp Leu Lys Leu Leu Lys Asp Thr Phe Ser Ser Asp 
                325                 330                 335     


Arg Lys Ile Tyr Asn Lys Ile Phe Arg Ser Lys Lys Asp Ile Lys Asp 
            340                 345                 350         


Asp Lys Asp Met Cys Leu Tyr Asp Leu Tyr Ile His Asn Lys Leu Glu 
        355                 360                 365             


Thr Thr Asp Phe Leu Lys Glu Ile Lys Lys Tyr Val Asn Thr Asp Asn 
    370                 375                 380                 


Phe Ile Ile Thr Asp Glu Asp Phe Leu Lys Arg Leu Glu Asn Asn Glu 
385                 390                 395                 400 


Val Leu Pro Arg Ile Thr Ser Lys Glu Asn Gly Lys Tyr Pro Tyr Gln 
                405                 410                 415     


Leu Asn Lys Asp Glu Leu Val Ser Ile Ile Glu His Gln Gly Lys His 
            420                 425                 430         


Tyr Pro Phe Leu Leu Asn Lys Thr Asp Asp Gly Ile Tyr Lys Leu Val 
        435                 440                 445             


Arg Leu Leu Glu Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Val Ser 
    450                 455                 460                 


Lys Asp Met Ser Glu Asn Ala Trp Met Leu Arg Asn Glu Gly Met Gln 
465                 470                 475                 480 


Gly Val His Ile Thr Pro Tyr Asn Phe Lys Lys Val Ile Asn Leu Asp 
                485                 490                 495     


Lys Thr Ala Glu Ile Phe Ile Lys Arg Met Ile Ser His Cys Thr Tyr 
            500                 505                 510         


Leu Leu Asp Glu Glu Ala Leu Pro Asn Asn Ser Ile Leu Tyr Ser Glu 
        515                 520                 525             


Phe Lys Val Leu Asn Glu Leu Lys Gln Ile Lys Val Asn Gly Glu His 
    530                 535                 540                 


Leu Thr Lys Asp Glu Gln His Lys Ile Ile Glu Glu Leu Phe Met Lys 
545                 550                 555                 560 


Thr Ser Gly Thr Ile Thr Glu Asn Lys Phe Arg Glu Phe Leu Leu Asn 
                565                 570                 575     


Thr Leu Asp Tyr Asn Phe Tyr Glu Asn Glu Leu Lys Ile Thr Gly Tyr 
            580                 585                 590         


Ser Ala Asp Cys Lys Phe Ala Asn Asn Met Gln Ser Tyr Ile Asp Phe 
        595                 600                 605             


Phe Gly Glu Asn Gly Ile Phe Glu Gly Thr Ser Tyr Asn Thr Asn Asp 
    610                 615                 620                 


Ala Glu Lys Ile Ile Glu Trp Ile Thr Ile Phe Asp Asp Lys Asp Ile 
625                 630                 635                 640 


Leu Lys Arg Lys Val Glu Asn Glu Tyr Pro Glu Leu Lys Ser Asn Gln 
                645                 650                 655     


Val Lys Asn Ile Leu Asn Lys Lys Tyr Ser Gly Trp Gly Arg Leu Ser 
            660                 665                 670         


Arg Lys Leu Leu Leu Asp Leu Arg Val Leu Asp Lys Glu Thr Asp Ile 
        675                 680                 685             


Pro Lys Ser Ile Met Asp Leu Met Tyr Glu Thr Asp Glu Asn Phe Met 
    690                 695                 700                 


Gln Ile Ile Asn Asn Asp Lys Tyr Asn Phe Gln Lys Leu Ile Ala Lys 
705                 710                 715                 720 


Glu Asn Lys Ile Glu Asn Thr Ser Asn Asp Leu Ser Tyr Asp Val Val 
                725                 730                 735     


Glu Asn Leu Ala Thr Ser Pro Ala Thr Lys Arg Gly Ile Tyr Gln Ala 
            740                 745                 750         


Leu Lys Val Val Lys Glu Ile Thr Thr Tyr Met Gly Tyr Glu Pro Lys 
        755                 760                 765             


Asn Ile Met Val Glu Met Ala Arg Gly Asp Glu Lys Lys Ile Arg Lys 
    770                 775                 780                 


Asp Asp Arg Lys Lys Tyr Leu Gln Lys Leu Tyr Asp Asn Ile Lys Asp 
785                 790                 795                 800 


Glu Val Asp Ser Asn Tyr Lys Asn Leu Lys Lys Glu Leu Ala Lys Glu 
                805                 810                 815     


Glu Lys Ile Asp Thr Asp Lys Leu Tyr Leu Tyr Tyr Leu Gln Glu Gly 
            820                 825                 830         


Lys Cys Leu Tyr Cys Gly Lys Pro Ile Asn Val Asp Asp Leu Asn Thr 
        835                 840                 845             


Lys Asn Ser Leu Tyr Glu Ile Asp His Ile Leu Pro Arg Thr Leu Ile 
    850                 855                 860                 


Lys Asp Asn Ser Trp Asp Asn Arg Val Leu Val Cys Arg Gly Cys Asn 
865                 870                 875                 880 


Gln Lys Lys Ala Ala Ser Leu Val Leu Pro Lys Glu Phe Arg Asn Asp 
                885                 890                 895     


Tyr Thr Lys Ser Ile Trp Ile Arg Leu Lys Asn Asn Lys Leu Met Ser 
            900                 905                 910         


Asn Lys Lys Phe Tyr Asn Leu Thr Arg Lys Ser Tyr Ser Glu Glu Asp 
        915                 920                 925             


Ile Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
    930                 935                 940                 


Lys His Val Ala Asn Ile Leu Asn Cys Phe Tyr Pro Lys Thr Lys Ile 
945                 950                 955                 960 


Val Tyr Leu Lys Ala Asn Ile Ser His Ser Tyr Arg Asp Lys Tyr Asp 
                965                 970                 975     


Leu Phe Lys Phe Arg Asp Ile Asn Asp Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Ala Ala Thr Leu Gly Ser  Tyr Gln Glu Lys Tyr  Leu His Tyr 
        995                 1000                 1005             


Asp Leu  Asp Tyr Asn Lys Ile  Asn Glu Phe Asn Glu  Lys Leu Ile 
    1010                 1015                 1020             


Lys Asn  Glu Asp Tyr Lys Arg  Leu His Tyr Gly Ile  Val Val Asn 
    1025                 1030                 1035             


Ser Leu  Asp Glu Glu Phe Gln  Ser Ala Phe Ala Glu  Ile Ala Pro 
    1040                 1045                 1050             


Arg Leu  Ile Asn Glu Glu Thr  Gly Glu Leu Leu Phe  Asn Ala Lys 
    1055                 1060                 1065             


Lys Phe  Asn Asp Leu Val Glu  Asp Thr Leu Tyr Arg  Asn Asp Ile 
    1070                 1075                 1080             


Leu Ile  Ser Arg Lys Thr Glu  Ile Arg Thr Gly Lys  Leu Tyr Lys 
    1085                 1090                 1095             


Glu Thr  Ile Tyr Lys Arg Gly  Lys Gly Asn Ile Lys  Ile Lys Glu 
    1100                 1105                 1110             


Asn Met  Pro Thr Glu Thr Tyr  Gly Gly Tyr Ser Asn  Met Glu Thr 
    1115                 1120                 1125             


Ser Tyr  Leu Thr Leu Val Lys  Tyr Gly Asn Lys Thr  Lys Leu Ile 
    1130                 1135                 1140             


Gly Ile  Pro Met Ser Ile Ala  Asn Asn Lys Asp Val  Lys Val Lys 
    1145                 1150                 1155             


Leu Asp  Phe Ile Lys Glu His  Leu Asp Leu Lys Ser  Leu Asp Asn 
    1160                 1165                 1170             


Ile Val  Ile Leu Lys Asp Asn  Ile Pro Tyr Asp Val  Ser Ile Asn 
    1175                 1180                 1185             


Tyr Lys  Asn Gln Asp Val Tyr  Val Lys Gly Tyr Ser  Thr Ala Asn 
    1190                 1195                 1200             


Lys Asn  Cys Glu Leu Ser Asn  Ala His Gln Leu Lys  Ile Lys Lys 
    1205                 1210                 1215             


Asp Met  Met Lys Lys Cys Lys  Tyr Val Leu Asn Lys  Val Tyr Lys 
    1220                 1225                 1230             


Asn Lys  Tyr Asp Asp Ile Thr  Asn Met Asp Ile Glu  Asn Ala Ile 
    1235                 1240                 1245             


Thr Phe  Ile Asn Tyr Leu Phe  Glu Ile Lys Ile Glu  Phe Pro Leu 
    1250                 1255                 1260             


Phe Ser  Lys Glu Ile Thr Lys  Ile Gln Asp Asn Ile  Asn Leu Thr 
    1265                 1270                 1275             


Glu Cys  Ser Phe Glu Gln Leu  Ala Asn Ile Ile Lys  Gln Leu Leu 
    1280                 1285                 1290             


Ile Ile  Tyr His Cys Asn Ser  Lys Asn Gly Asn Leu  Lys Glu Phe 
    1295                 1300                 1305             


Gly Leu  Ser Asp Arg Ile Gly  Arg Leu Ser Gly Ala  Asn Val Thr 
    1310                 1315                 1320             


Gly Gly  Asp Phe Ile Phe Thr  Ser Val Thr Gly Leu  Lys Glu Lys 
    1325                 1330                 1335             


Thr Ala  Ser Tyr Asp Gly Glu  
    1340                 1345 


<210>  889
<211>  1345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  889

Met Val Glu Lys Tyr Gly Ile Gly Leu Asp Ile Gly Thr Ser Thr Leu 
1               5                   10                  15      


Gly Trp Thr Ser Val Asp Glu Asn Gly Lys Ile Gln Arg Leu Lys Gly 
            20                  25                  30          


Gln Thr Ala Leu Gly Thr Arg Leu Phe Asn Glu Gly Gln Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Ile Gln Arg Arg Arg 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Ile Phe Asp Glu Pro Ile Gly Ser 
65                  70                  75                  80  


Val Asp Ala Gly Phe Phe Ala Arg Gln Lys Met Ser Ser Leu Ser Pro 
                85                  90                  95      


Gln Asp Asp Arg Phe Glu Asp Thr Tyr Arg Leu Phe Asn Asn Gln Thr 
            100                 105                 110         


Asp Lys Ala Phe His Asp Glu Tyr Pro Thr Thr Tyr His Leu Arg Gln 
        115                 120                 125             


Ala Leu Met Asn Ser Asp Arg Gln Phe Asp Ile Arg Glu Ile Tyr Leu 
    130                 135                 140                 


Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Arg Ser Gly 
145                 150                 155                 160 


Lys Pro Ala Asp Phe Lys Thr Gly Lys Leu Asp Leu Lys Glu Lys Phe 
                165                 170                 175     


Glu Glu Ile Asn Ala Asp Trp Gln Thr Leu Ile Ser Glu Asp Ala Pro 
            180                 185                 190         


Gln Leu Pro Val Asp Asp Leu Thr Thr Ile Lys Ala Val Leu Val Asp 
        195                 200                 205             


Gly Ser Arg Ser Arg Ser Asp Arg Gln Arg Gly Leu Val Lys Leu Trp 
    210                 215                 220                 


Met Ala Asn Glu Thr Val Glu Lys Asn Trp Lys Pro Val Met Thr Glu 
225                 230                 235                 240 


Leu Ala Lys Ala Ile Leu Gly Leu Lys Thr Lys Leu Tyr Leu Leu Thr 
                245                 250                 255     


Gly Leu Asp Leu Pro Lys Glu Asp Gln Lys Ala Trp Thr Phe Ser Leu 
            260                 265                 270         


Asp Thr Leu Glu Asp His Gln Gly Asp Ile Asp Ser Phe Phe Asp Asp 
        275                 280                 285             


His Ala Gln Gln Leu Ile Asp Arg Leu Ala Glu Leu Arg Ala Met Ile 
    290                 295                 300                 


Gln Leu Thr Glu Ile Ile Pro Ser Gly Leu Asn Phe Ser Gln Ser Met 
305                 310                 315                 320 


Val Gln Arg Tyr Glu Ala His Arg Ser His Leu Glu Met Tyr Lys Gln 
                325                 330                 335     


Tyr Ala Leu Asp Gln Thr Asp Pro Lys Arg Thr Lys Ala Leu Arg Ala 
            340                 345                 350         


Ala Tyr Asn Ala Tyr Ile Asp Gln His Glu Ile Ala Asp Asp Phe Tyr 
        355                 360                 365             


Thr Ala Val Lys Arg Val Thr Lys Asp Asp Gln Ser Glu Thr Ala Glu 
    370                 375                 380                 


Glu Ile Asn Arg Leu Ile Gly Leu Glu Gln Phe Met Pro Lys Gln Arg 
385                 390                 395                 400 


Thr Lys Glu Asn Gly Val Ile Pro Tyr Gln Val His Gln Gln Glu Leu 
                405                 410                 415     


Asp Gln Ile Ile Ala His Gln Lys Lys Tyr Tyr Pro Phe Leu Gly Thr 
            420                 425                 430         


Glu Asn Pro Val Ile Asp His Arg Glu Ser Gln Pro Tyr Met Leu Asp 
        435                 440                 445             


Glu Leu Val Ser Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Val Ala 
    450                 455                 460                 


Ala Asn Pro Glu Leu Lys Thr Thr Glu Asn Pro Phe Ala Trp Met Ile 
465                 470                 475                 480 


Arg Lys Ala Gln Gly Glu Ile Thr Pro Trp Asn Phe Asp Glu Lys Val 
                485                 490                 495     


Asp Arg Lys Gly Ser Ala Thr Ala Phe Ile Glu Arg Met Lys Thr Thr 
            500                 505                 510         


Asp Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Gln Gln Ser Leu Leu 
        515                 520                 525             


Tyr Gln Arg Phe Met Val Leu Asn Glu Leu Asn Asn Ile Arg Ile Asp 
    530                 535                 540                 


Asp Glu Leu Leu Thr Thr Asn Gln Lys Gln Trp Val Phe Asp Arg Val 
545                 550                 555                 560 


Phe Lys Gly Lys Gly Thr Ile Ser Ala Lys His Leu Gln Asp Ala Leu 
                565                 570                 575     


Lys His His Gly Glu Thr Asp Gly Leu Pro Lys Ile Ser Gly Leu Ala 
            580                 585                 590         


Asp Pro Thr Lys Phe Asn Ser Ala Leu Thr Ser Tyr Arg Asp Phe Gln 
        595                 600                 605             


Lys Trp Leu Pro Asn Asp Ile Asp Arg Lys Asp Arg Thr Ala Asp Ile 
    610                 615                 620                 


Glu Thr Ile Ile Asn Trp Ser Thr Val Phe Glu Asp Thr His Ile Phe 
625                 630                 635                 640 


Val Glu Lys Leu Lys Glu Ile Glu Trp Leu Thr Ala Asp Gln Arg Arg 
                645                 650                 655     


Arg Ile Ala Ser Asn Arg Tyr Arg Gly Trp Gly Arg Leu Ser Lys His 
            660                 665                 670         


Leu Leu Thr Glu Ile Lys Asp Glu Gln Gly Leu Ser Ile Met Asp Gln 
        675                 680                 685             


Leu Trp Arg Thr Gln His Asn Phe Met Gln Ile Val His Glu Pro Asp 
    690                 695                 700                 


Tyr Ala Lys Gln Ile Glu Ala Ala Asn Thr Val Gly Met Ala Lys Gln 
705                 710                 715                 720 


Asp Ile Gly Thr Val Ile Asn Asp Leu Tyr Thr Ser Pro Gln Asn Lys 
                725                 730                 735     


Lys Ala Ile Arg Gln Val Leu Ala Val Val Lys Asp Ile Gln Asn Ala 
            740                 745                 750         


His His Gly Val Ala Pro Ser Trp Ile Phe Met Glu Ala Ala Arg Gly 
        755                 760                 765             


Ala Asp Gln Asn Pro Gln Arg Ser Arg Gln Arg Gln Arg Gln Leu Gln 
    770                 775                 780                 


Asp Leu Tyr Ala Lys Thr Ala Ala Asp Leu Val Asp Thr Glu Val Gln 
785                 790                 795                 800 


Gln Glu Leu Thr Asp Lys Ile Lys Asp Lys Ala Asn Phe Thr Asp Arg 
                805                 810                 815     


Leu Met Leu Tyr Phe Leu Gln Asn Gly Gln Asp Met Tyr Ser Lys Glu 
            820                 825                 830         


Lys Leu Asn Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Leu 
        835                 840                 845             


Pro Gln Ala Leu Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val 
    850                 855                 860                 


His Gln Ser Ile Asn Arg Asn Lys Ser Asp Thr Phe Ala Ser Glu Lys 
865                 870                 875                 880 


Tyr Gly Ala Lys Met Gln Gly Ala Trp His Gln Trp Arg Ala Ala Gly 
                885                 890                 895     


Leu Ile Ser Ala Arg Lys Leu Arg Asn Leu Glu Met Arg Pro Asp Glu 
            900                 905                 910         


Ile Ser Lys Tyr Ala Thr Gly Phe Ile Arg Arg Gln Leu Val Glu Thr 
        915                 920                 925             


Arg Gln Val Ile Lys Leu Val Thr Glu Ile Leu Ser His Gln Tyr Asn 
    930                 935                 940                 


Pro Glu Glu Thr Lys Leu Val Ser Val Lys Ala Gly Leu Ser His Glu 
945                 950                 955                 960 


Leu Arg Arg Glu Ala Gly Leu Pro Lys Leu Arg Asp Leu Asn Asp Tyr 
                965                 970                 975     


His His Ala Ile Asp Ala Tyr Leu Ala Ala Arg Ile Gly Thr Tyr Leu 
            980                 985                 990         


Leu Lys Arg Tyr Pro Arg Leu Glu  Arg Phe Phe Val Tyr  Gly Gln Phe 
        995                 1000                 1005             


Gln Thr  Ser Arg Leu Asp Leu  Arg Arg Phe Asn Phe  Ile His Asp 
    1010                 1015                 1020             


Leu Val  Lys Ser Lys Gln Gly  Val Ile Val Asp Ser  Asp Thr Gly 
    1025                 1030                 1035             


Glu Ile  Leu Trp Asp Arg Gln  Lys Asp Ile Asp Tyr  Ile Lys His 
    1040                 1045                 1050             


Leu Met  Gly Leu Lys His Leu  Leu Val Thr His Glu  Val Tyr Asp 
    1055                 1060                 1065             


Ser Ala  Ala Glu Met Tyr Ala  Gln Thr Ile Phe Lys  Ala Ser Asp 
    1070                 1075                 1080             


Asn Asp  Ser Lys Val Leu Ile  Pro Thr Lys Asn Asp  Arg Pro Thr 
    1085                 1090                 1095             


Ala Ile  Tyr Gly Gly Tyr Thr  Lys Arg Arg Asn Gly  Tyr Leu Ala 
    1100                 1105                 1110             


Ile Leu  Lys Ile Thr Glu Lys  Lys Ala Glu Thr Tyr  Arg Val Val 
    1115                 1120                 1125             


Gly Val  Pro Thr Leu Tyr Met  Ser Gln Leu Ala Thr  Ala Arg Ser 
    1130                 1135                 1140             


His Ser  Val Glu Ala Glu Lys  Ala Leu Leu Lys Ser  Leu Met Ala 
    1145                 1150                 1155             


Pro Glu  Ile Leu Ser Glu Ser  Lys Val Val Ser Phe  Glu Val Val 
    1160                 1165                 1170             


Val Ser  His Val His Leu Asn  Gln Leu Val Arg Asp  Thr Val Phe 
    1175                 1180                 1185             


Gly Pro  Ala His Tyr Phe Gly  Leu Gly Pro Phe Arg  Tyr Val Arg 
    1190                 1195                 1200             


Asn Phe  His Gln Leu Val Leu  Pro Phe Lys Asp Gln  Glu Ile Leu 
    1205                 1210                 1215             


Val Lys  Arg Thr Pro Thr Asp  Glu Glu Leu Asn Thr  Val Phe Glu 
    1220                 1225                 1230             


Asp Ile  Cys Trp Gln Val Glu  Asn Tyr Phe Ala Val  Phe Asp Ile 
    1235                 1240                 1245             


Asn Gly  Phe Arg Asn Ala Leu  Lys Lys Ser Ile Asp  Lys Phe Glu 
    1250                 1255                 1260             


Gln Leu  Pro Ile Glu Asn Val  Phe Lys Asp Lys Lys  Leu Val Thr 
    1265                 1270                 1275             


Gln Gly  Lys Lys Glu Val Leu  Ala Phe Val Met Gln  Gly Leu His 
    1280                 1285                 1290             


Ala Asn  Ala Ser Tyr Arg Asn  Leu Lys Val Ser Leu  Gly Ile Lys 
    1295                 1300                 1305             


Thr Pro  Leu Gly Gln Leu Gln  Leu Thr Lys Phe Met  Leu Ser Arg 
    1310                 1315                 1320             


Asp Ala  Glu Leu Ile Asp Glu  Ser Pro Thr Gly Leu  Phe Arg Arg 
    1325                 1330                 1335             


Ile Arg  Arg Leu Lys Asp Leu  
    1340                 1345 


<210>  890
<211>  1345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  890

Met Gly Asn Lys Leu Arg Asn Val Asp Asp Tyr Tyr Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Thr Glu Ser Val Gly Trp Ala Val Thr Asp Lys Tyr Tyr Val 
            20                  25                  30          


Ile Pro Lys Phe Lys Gly Asn Ala Met Trp Gly Val His Leu Phe Asp 
        35                  40                  45              


Glu Gly Glu Thr Ser Val Lys Arg Arg Gly Phe Arg Ser Ala Arg Arg 
    50                  55                  60                  


Arg Thr Gln Arg Lys Arg Glu Arg Leu Ala Leu Leu Glu Ala Leu Phe 
65                  70                  75                  80  


Asp Glu Glu Ile Cys Lys Lys Asp Cys Thr Phe Phe Arg Arg Leu His 
                85                  90                  95      


Glu Ser Asp Leu Trp Pro Glu Asp Lys Ser Gly Asp Asp Lys Tyr Ala 
            100                 105                 110         


Val Phe Ala Asp Glu Asn Tyr Thr Asp Lys Asp Phe His Lys Glu Tyr 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Tyr Glu Leu Ile Lys Asn Lys Glu Pro 
    130                 135                 140                 


His Asp Val Lys Leu Val Phe Leu Ala Leu His His Ile Ile Lys Asn 
145                 150                 155                 160 


Arg Gly His Phe Leu Phe Asp Ser Leu Ser Ser Ala Glu Asp Phe Asn 
                165                 170                 175     


Ser Ala Tyr Glu Thr Met Cys Ser Tyr Leu Ser Asp Asn Glu Ile Gln 
            180                 185                 190         


Leu Asn Cys Ser Asp Ile Glu Gln Phe Lys Ala Ile Leu Lys Asp Arg 
        195                 200                 205             


Arg Gln Gly Lys Ile Glu Lys Thr Lys Asn Leu Ala Ser Leu Phe Gly 
    210                 215                 220                 


Ile Thr Lys Lys Ser Asp Pro Val Ser Tyr Ala Val Met Ala Val Leu 
225                 230                 235                 240 


Ser Gly Ser Thr Val Lys Met Cys Asp Leu Phe Gly Asp Asp Glu Leu 
                245                 250                 255     


Lys Thr Val Glu Lys Asn Asn Ile Ser Phe Ala Ser Gly Tyr Glu Glu 
            260                 265                 270         


Asn Glu Glu Ile Tyr Ala Ser Val Leu Gly Asp Arg Phe Glu Leu Val 
        275                 280                 285             


Lys Ile Leu Lys Ala Val Tyr Asp Trp Ala Val Leu Ala Asp Ile Leu 
    290                 295                 300                 


Asp Gly Lys Lys Tyr Ile Ser Glu Ala Lys Val Ala Val Tyr Glu Lys 
305                 310                 315                 320 


His Lys Asn Asp Leu Lys Thr Leu Lys Lys Tyr Val Lys Thr Tyr Leu 
                325                 330                 335     


Pro Glu Lys Tyr Asn Glu Ile Phe Lys Ile Ser Asp Gly Lys Lys Asp 
            340                 345                 350         


Asn Tyr Val Ala Tyr Ser Gly His Ile Lys Glu Ser Gly Lys Thr Gly 
        355                 360                 365             


Glu Leu Lys Lys Thr Cys Asn Ser Glu Asn Phe Cys Ala Tyr Leu Lys 
    370                 375                 380                 


Lys Thr Leu Gly Lys Asn Ala Asp Leu Ser Tyr Lys Glu Met Phe Asp 
385                 390                 395                 400 


Glu Ile Glu Asn Lys Thr Phe Met Pro Lys Gln Val Thr Lys Ser Asn 
                405                 410                 415     


Gly Val Val Pro Met Gln Val Asn Glu Glu Glu Leu Lys Ala Ile Leu 
            420                 425                 430         


Asp Asn Ala Lys Asp Tyr Leu Ser Phe Leu Asn Ser Cys Asp Glu Asn 
        435                 440                 445             


Gly Val Ser Val Tyr Asp Lys Ile Ile Lys Ile Phe Glu Tyr Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Asn Thr His Thr Glu Asn Ser Trp Leu 
465                 470                 475                 480 


Lys Arg Lys Asp Gly Val Lys Ile Tyr Pro Trp Asn Phe Asp Asp Val 
                485                 490                 495     


Val Asp Ile Asp Ser Ser Ala Glu Lys Phe Ile Asn Asn Leu Thr Ser 
            500                 505                 510         


Lys Cys Thr Tyr Leu Pro Glu Cys Asp Val Ile Pro Lys Cys Ser Leu 
        515                 520                 525             


Leu Tyr Ser Lys Phe Thr Val Leu Asn Glu Ile Asn Asn Leu Lys Leu 
    530                 535                 540                 


Asn Gly Glu Asp Ile Pro Val Glu Leu Lys Gln Gly Ile Tyr Lys Asp 
545                 550                 555                 560 


Leu Phe Met Thr Arg Arg Lys Val Thr Val Lys Gln Ile Lys Asp Tyr 
                565                 570                 575     


Leu Lys Ala Arg Gly Cys Tyr Asp Asn Asn Asp Val Leu Ser Gly Ile 
            580                 585                 590         


Asp Thr Thr Val Lys Ser Ser Leu Lys Ser Tyr Phe Asp Phe Lys Gly 
        595                 600                 605             


Phe Glu Ser Leu Thr Phe Asp Asp Lys Glu Ser Ile Ile Ala Ser Ser 
    610                 615                 620                 


Val Ile Phe Gly Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Lys Lys 
625                 630                 635                 640 


Leu Phe Pro Glu Lys Leu Thr Asp Glu Asp Ile Lys Lys Ile Cys Lys 
                645                 650                 655     


Leu Lys Tyr Thr Gly Trp Ser Arg Leu Ser Lys Glu Phe Leu Cys Asp 
            660                 665                 670         


Val Glu Phe Thr Tyr Lys Lys Thr Gly Glu Val Met Asn Val Ile Thr 
        675                 680                 685             


Ala Leu Trp Glu Thr Asn Tyr Asn Leu Met Gln Ile Leu Tyr Ser Glu 
    690                 695                 700                 


Asp Phe Cys Cys Asp Gly Asp Glu Lys Lys Asn Leu Asn Asp Lys Leu 
705                 710                 715                 720 


Asp Glu Leu Ser Tyr Thr Gly Asp Gln His Ser Leu Arg Lys Ala Val 
                725                 730                 735     


Asp Ala Leu Tyr Val Ser Pro Lys Val Lys Arg Pro Ile Leu Arg Ala 
            740                 745                 750         


Leu Leu Ile Ala Lys Glu Ile Glu Lys Ile Gln Glu Arg Pro Pro Lys 
        755                 760                 765             


Lys Ile Phe Val Glu Val Ala Arg Gly Ile Asp Asn Arg Glu Glu Arg 
    770                 775                 780                 


His Arg Asp Ser Arg Lys Asn Arg Leu Met Glu Leu Tyr Lys Asn Cys 
785                 790                 795                 800 


Lys Lys Glu Ala Glu Lys Phe Cys Pro Tyr Phe Asp Ala Leu Ser Lys 
                805                 810                 815     


Lys Leu Ser Asp Glu Asp Glu Ser Lys Leu Arg Arg Asp Lys Leu Tyr 
            820                 825                 830         


Phe Tyr Tyr Ile Gln Leu Gly Lys Cys Met Tyr Thr Gly Glu Glu Ile 
        835                 840                 845             


Ser Leu Gly Glu Leu Met Asn Gly Asn Gly Arg Phe Asp Ile Asp His 
    850                 855                 860                 


Ile Tyr Pro Arg Ser Lys Ile Lys Asp Asp Ser Leu Thr Asn Lys Val 
865                 870                 875                 880 


Leu Val Asn Lys Thr Val Asn Asn Asn Lys Thr Asn Val Tyr Pro Ile 
                885                 890                 895     


Asp Ala Ala Ile Gln Ser Lys Met Ser Pro Tyr Trp Gln Met Leu Arg 
            900                 905                 910         


Ser Ser Gly Leu Ile Ser Glu Glu Thr Tyr Lys Arg Leu Thr Arg Lys 
        915                 920                 925             


Thr Pro Leu Thr Asp Asp Glu Leu Ser Ala Phe Val Ser Arg Gln Leu 
    930                 935                 940                 


Thr Glu Thr Gln Gln Ser Thr Lys Ala Val Ala His Ile Leu Glu Gln 
945                 950                 955                 960 


Leu Tyr Pro Lys Pro Lys Thr Glu Ile Val Tyr Val Lys Ala Ala Leu 
                965                 970                 975     


Val His Ala Phe Arg Gln Ser Asn Asp Met Leu Lys Cys Arg Glu Val 
            980                 985                 990         


Asn Asp Leu His His Ala Lys Asp  Ala Tyr Leu Asn Ile  Val Val Gly 
        995                 1000                 1005             


Asn Ala  Tyr Asn Val Lys Val  Thr His Asn Lys Val  Asn Phe Ile 
    1010                 1015                 1020             


Lys Gly  Leu Gln Asn Asn Asp  Lys Gly Tyr Thr Val  Asn Pro Glu 
    1025                 1030                 1035             


Lys Phe  Phe Ser His Asp Ile  Pro Gly Ala Trp Ser  Val Lys Asp 
    1040                 1045                 1050             


Ser Met  Asn Thr Val Arg Arg  Phe Met Asn Lys Asn  Asn Ile Arg 
    1055                 1060                 1065             


Tyr Thr  Arg Phe Pro Arg Lys  Lys Gln Gly Gly Leu  Phe Lys Ile 
    1070                 1075                 1080             


Asn Pro  Leu Lys Lys Gly Asn  Gly Gln Val Pro Leu  Lys Ala Ser 
    1085                 1090                 1095             


Gly Ala  Arg Ser Cys Ile Glu  Lys Tyr Gly Gly Tyr  Asp Lys Ala 
    1100                 1105                 1110             


Thr Ser  Ala Tyr Phe Ser Tyr  Val Glu Tyr Thr Asp  Lys Lys Gly 
    1115                 1120                 1125             


Lys Ala  Val Arg Gln Met Phe  Pro Val Asn Leu Tyr  Glu Val Lys 
    1130                 1135                 1140             


Glu Tyr  Glu Ala Asp Met Gln  Gly Phe Val Thr Ser  Arg Ile Ala 
    1145                 1150                 1155             


Glu Ala  Ser Asn Val Lys Ile  Leu Ile Pro Cys Val  Lys Tyr Asp 
    1160                 1165                 1170             


Ala Cys  Ile Ser Phe Asp Gly  Cys Arg Met Phe Ile  Thr Lys Lys 
    1175                 1180                 1185             


Thr Gly  Asn Arg Ile Gly Tyr  Lys Pro Gly Met Gln  Leu Val Leu 
    1190                 1195                 1200             


Gly Tyr  Lys Tyr Glu Lys Tyr  Ile Arg Asn Ile Glu  Asn Tyr Leu 
    1205                 1210                 1215             


Thr Lys  His Lys Asp Arg Glu  Ile Ile Pro Arg Asp  Asn Ile Ser 
    1220                 1225                 1230             


Ala Glu  Glu Asn Ile Glu Leu  Tyr Asp Ile Leu Thr  Gly Lys Met 
    1235                 1240                 1245             


Leu Asn  Thr Val Leu Ser Val  Lys Phe Ser Gly Ile  Gly Lys Lys 
    1250                 1255                 1260             


Leu Glu  Lys Arg Arg Glu Lys  Phe Val Leu Cys Ser  Pro Glu Glu 
    1265                 1270                 1275             


Gln Cys  Cys Val Leu Asn Glu  Ile Leu Lys Ile Leu  His Ala Asn 
    1280                 1285                 1290             


Val Leu  Ser Gly Asp Leu Ser  Leu Ile Gly Glu Ser  Pro Asn Thr 
    1295                 1300                 1305             


Gly Thr  Met Lys Thr Asn Ala  Lys Phe Ser Glu Ile  Lys Asp Val 
    1310                 1315                 1320             


His Ser  Ile Lys Leu Ile Asn  Gln Ser Val Thr Gly  Leu Phe Glu 
    1325                 1330                 1335             


Gln Glu  Ile Asp Leu Leu Lys  
    1340                 1345 


<210>  891
<211>  1345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  891

Met Gly Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Thr Gly Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         


Gly Glu His His Pro Ile Phe Gly Asn Ile Ala Ala Glu Val Lys Tyr 
        115                 120                 125             


His Asp Asp Phe Pro Thr Ile Tyr His Leu Arg Arg His Leu Ala Asp 
    130                 135                 140                 


Thr Ser Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Tyr Glu Gly Asp Leu Lys Ala 
                165                 170                 175     


Glu Asn Thr Asp Val Gln Ala Leu Phe Lys Asp Phe Val Glu Glu Tyr 
            180                 185                 190         


Asp Lys Thr Ile Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             


Leu Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Ser Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Asp Leu His Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     


Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Asp Leu Glu Gly Phe Leu Gly Glu Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             


Leu Phe Ala Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Val Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Lys Lys Leu Lys 
                325                 330                 335     


Asp Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Ala Ile Phe Lys 
            340                 345                 350         


Asp Lys Asn Lys Lys Gly Tyr Ala Ser Tyr Ile Glu Ser Gly Val Lys 
        355                 360                 365             


Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Leu Lys Ile Asn 
    370                 375                 380                 


Gly Ser Gly Asp Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         


Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 


Ala Glu Tyr Lys Ala Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 


Ile Leu Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     


Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Pro Leu Tyr Glu Ala Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Asn Glu Gln Gly Glu Ala Lys Phe Phe Asp Thr Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 


Asp Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     


Val Asn Leu Thr Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ser Ser 
            580                 585                 590         


Leu Gly Thr Tyr His Asp Leu Arg Lys Ile Leu Asp Lys Ser Phe Leu 
        595                 600                 605             


Asp Asp Lys Ala Asn Glu Lys Thr Ile Glu Asp Ile Ile Gln Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr 
625                 630                 635                 640 


Ser Asp Ile Phe Thr Lys Ala Gln Leu Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Asn Lys Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
        675                 680                 685             


Tyr Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser 
    690                 695                 700                 


Phe Lys Glu Glu Ile Ala Arg Ala Gln Ile Ile Asp Asp Val Asp Asp 
705                 710                 715                 720 


Ile Ala Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly His Asn Pro Ala Asn Ile Ile Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Asp Lys Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Leu Leu 
    770                 775                 780                 


Gln Asp Ser Leu Lys Asn Leu Asp Asn Pro Val Asn Ile Lys Asn Val 
785                 790                 795                 800 


Glu Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln 
                805                 810                 815     


Asn Gly Lys Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asn Asn Leu 
            820                 825                 830         


Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp 
        835                 840                 845             


Asn Ser Leu Asp Asn Arg Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asp Val Pro Ser Ile Glu Val Val His Glu Met Lys Ser 
865                 870                 875                 880 


Phe Trp Ser Lys Leu Leu Ser Val Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Glu Arg Phe Asn Thr Glu Phe Asp Gly 
    930                 935                 940                 


Asn Lys Arg Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn 
945                 950                 955                 960 


Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Asn Ala Leu Leu Leu Lys Tyr  Pro Gln Leu Glu Pro  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Glu Tyr Pro Lys Tyr  Asn Ser Tyr Arg Ser  Arg Lys Ser 
    1010                 1015                 1020             


Ala Thr  Glu Lys Phe Leu Phe  Tyr Ser Asn Ile Leu  Arg Phe Phe 
    1025                 1030                 1035             


Lys Lys  Glu Asp Ile Gln Thr  Asn Glu Asp Gly Glu  Ile Ala Trp 
    1040                 1045                 1050             


Asn Lys  Glu Lys His Ile Lys  Ile Leu Arg Lys Val  Leu Ser Tyr 
    1055                 1060                 1065             


Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu Glu Gln  Thr Gly Gly 
    1070                 1075                 1080             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly Glu Ser  Asp Lys Leu 
    1085                 1090                 1095             


Ile Pro  Arg Lys Thr Lys Asn  Ser Tyr Trp Asp Pro  Lys Lys Tyr 
    1100                 1105                 1110             


Gly Gly  Phe Asp Ser Pro Val  Val Ala Tyr Ser Ile  Leu Val Phe 
    1115                 1120                 1125             


Ala Asp  Val Glu Lys Gly Lys  Ser Lys Lys Leu Arg  Lys Val Gln 
    1130                 1135                 1140             


Asp Met  Val Gly Ile Thr Ile  Met Glu Lys Lys Arg  Phe Glu Lys 
    1145                 1150                 1155             


Asn Pro  Val Asp Phe Leu Glu  Gln Arg Gly Tyr Arg  Asn Val Arg 
    1160                 1165                 1170             


Leu Glu  Lys Ile Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu 
    1175                 1180                 1185             


Glu Asn  Lys Arg Arg Arg Leu  Leu Ala Ser Ala Lys  Glu Leu Gln 
    1190                 1195                 1200             


Lys Gly  Asn Glu Leu Val Ile  Pro Gln Arg Phe Thr  Thr Leu Leu 
    1205                 1210                 1215             


Tyr His  Ser Tyr Arg Ile Glu  Lys Asp Tyr Glu Pro  Glu His Arg 
    1220                 1225                 1230             


Glu Tyr  Val Glu Lys His Lys  Asp Glu Phe Lys Glu  Leu Leu Glu 
    1235                 1240                 1245             


Tyr Ile  Ser Val Phe Ser Arg  Lys Tyr Val Leu Ala  Asp Asn Asn 
    1250                 1255                 1260             


Leu Thr  Lys Ile Glu Met Leu  Phe Ser Lys Asn Lys  Asp Ala Glu 
    1265                 1270                 1275             


Val Ser  Ser Leu Ala Lys Ser  Phe Ile Ser Leu Leu  Thr Phe Thr 
    1280                 1285                 1290             


Ala Phe  Gly Ala Pro Ala Ala  Phe Asn Phe Phe Gly  Glu Asn Ile 
    1295                 1300                 1305             


Asp Arg  Lys Arg Tyr Thr Ser  Val Thr Glu Cys Leu  Asn Ala Thr 
    1310                 1315                 1320             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1325                 1330                 1335             


Leu Ser  Lys Leu Gly Glu Asp  
    1340                 1345 


<210>  892
<211>  1345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  892

Met Asp Gln Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         


Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Ala Glu Val Lys Tyr 
        115                 120                 125             


His Asp Asp Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ile Ser Gln Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Gln Leu Lys Ala 
                165                 170                 175     


Glu Asn Thr Asn Val Gln Ala Leu Phe Lys Asp Phe Val Glu Val Tyr 
            180                 185                 190         


Asp Lys Thr Val Glu Glu Ser His Leu Ser Glu Met Thr Val Asp Ala 
        195                 200                 205             


Leu Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala His Tyr Pro Ala Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     


Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Asp Leu Glu Gly Leu Leu Gly Glu Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             


Leu Phe Ala Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Val Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Lys Lys Leu Lys 
                325                 330                 335     


Asp Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Ala Ile Phe Lys 
            340                 345                 350         


Asp Lys Asn Lys Lys Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             


Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Leu Gln Ile Asn 
    370                 375                 380                 


Gly Ser Gly Asp Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met His Ala Ile Leu Arg Arg Gln Glu Glu His Tyr Pro Phe 
            420                 425                 430         


Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 


Ala Glu Tyr Lys Ala Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 


Ile Leu Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     


Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Asn Glu Gln Gly Glu Ala Lys Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 


Asp Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     


Val Asn Leu Thr Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ser Ser 
            580                 585                 590         


Leu Gly Thr Tyr His Asp Leu Arg Lys Ile Leu Asp Lys Ser Phe Leu 
        595                 600                 605             


Asp Asp Lys Ala Asn Glu Lys Thr Ile Glu Asp Ile Ile Gln Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr 
625                 630                 635                 640 


Ser Asp Ile Phe Thr Lys Ala Gln Leu Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Asn Lys Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
        675                 680                 685             


Tyr Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser 
    690                 695                 700                 


Phe Lys Glu Glu Ile Ala Arg Ala Gln Ile Ile Gly Asp Val Asp Asp 
705                 710                 715                 720 


Ile Ala Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly His Asn Pro Ala Asn Ile Ile Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Met Thr Asp Lys Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Leu Leu 
    770                 775                 780                 


Gln Asp Ser Leu Lys Asn Leu Asp Asn Pro Val Asn Ile Lys Asn Val 
785                 790                 795                 800 


Glu Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln 
                805                 810                 815     


Asn Gly Lys Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asn Asn Leu 
            820                 825                 830         


Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp 
        835                 840                 845             


Asn Ser Leu Asp Asn Arg Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asp Val Pro Ser Ile Glu Val Val His Glu Met Lys Ser 
865                 870                 875                 880 


Phe Trp Ser Lys Leu Leu Ser Val Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Glu Arg Phe Asn Thr Glu Phe Asp Gly 
    930                 935                 940                 


Asn Lys Arg Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn 
945                 950                 955                 960 


Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Asn Ala Leu Leu Leu Lys Tyr  Pro Gln Leu Glu Pro  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Glu Tyr Pro Lys Tyr  Asn Ser Tyr Arg Ser  Arg Lys Ser 
    1010                 1015                 1020             


Ala Thr  Glu Lys Phe Leu Phe  Tyr Ser Asn Ile Leu  Arg Phe Phe 
    1025                 1030                 1035             


Lys Lys  Glu Asp Ile Gln Thr  Asn Glu Asp Gly Glu  Ile Ala Trp 
    1040                 1045                 1050             


Asn Lys  Glu Lys His Ile Lys  Ile Leu Arg Lys Val  Leu Ser Tyr 
    1055                 1060                 1065             


Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu Glu Gln  Thr Gly Gly 
    1070                 1075                 1080             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly Glu Ser  Asp Lys Leu 
    1085                 1090                 1095             


Ile Pro  Arg Lys Thr Lys Asn  Ser Tyr Trp Asn Pro  Lys Lys Tyr 
    1100                 1105                 1110             


Gly Gly  Phe Asp Ser Pro Val  Val Ala Tyr Ser Ile  Leu Val Phe 
    1115                 1120                 1125             


Ala Asp  Val Glu Lys Gly Lys  Ser Lys Lys Leu Arg  Lys Val Gln 
    1130                 1135                 1140             


Asp Met  Val Gly Ile Thr Ile  Met Glu Lys Lys Arg  Phe Glu Lys 
    1145                 1150                 1155             


His Pro  Val Asp Phe Leu Glu  Gln Arg Gly Tyr Arg  Asn Val Arg 
    1160                 1165                 1170             


Leu Glu  Lys Ile Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu 
    1175                 1180                 1185             


Glu Asn  Lys Arg Arg Arg Leu  Leu Ala Ser Ala Arg  Glu Leu Gln 
    1190                 1195                 1200             


Lys Gly  Asn Glu Leu Val Ile  Pro Gln Arg Phe Thr  Thr Leu Leu 
    1205                 1210                 1215             


Tyr His  Ser Tyr Gln Ile Glu  Lys Asn Tyr Glu Pro  Glu His Arg 
    1220                 1225                 1230             


Glu Tyr  Val Glu Lys His Lys  Asp Glu Phe Lys Glu  Leu Leu Glu 
    1235                 1240                 1245             


Tyr Ile  Ser Val Phe Ser Arg  Lys Tyr Val Leu Ala  Asp Asn Asn 
    1250                 1255                 1260             


Leu Thr  Lys Ile Glu Met Leu  Phe Ser Lys Asn Lys  Asp Ala Glu 
    1265                 1270                 1275             


Val Ser  Ser Leu Ala Lys Ser  Phe Ile Ser Leu Leu  Thr Phe Thr 
    1280                 1285                 1290             


Ala Phe  Gly Ala Pro Ala Ala  Phe Asn Phe Phe Gly  Glu Asn Ile 
    1295                 1300                 1305             


Asp Arg  Lys Arg Tyr Thr Ser  Val Thr Glu Cys Leu  Asn Ala Thr 
    1310                 1315                 1320             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1325                 1330                 1335             


Leu Ser  Lys Leu Gly Glu Asp  
    1340                 1345 


<210>  893
<211>  1345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  893

Met Lys Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Thr Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Glu Glu Asp Lys Gln 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Gly Thr Leu Lys Glu Glu Lys Glu Tyr 
        115                 120                 125             


His Lys Lys Phe Lys Thr Ile Tyr His Leu Arg Glu Glu Leu Ala Asn 
    130                 135                 140                 


Ser Lys Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Tyr Glu Gly Asp Leu Lys Ala 
                165                 170                 175     


Glu Asn Thr Asp Val Gln Ala Leu Phe Lys Asp Phe Val Glu Glu Tyr 
            180                 185                 190         


Asp Lys Thr Ile Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             


Leu Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Ser Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     


Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Asp Leu Glu Glu Leu Leu Gly Glu Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             


Leu Phe Ala Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Ala Val Asp Asp Asn Thr Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Val Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Lys Lys Leu Lys 
                325                 330                 335     


Asp Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Ala Ile Phe Lys 
            340                 345                 350         


Asp Lys Asn Lys Lys Gly Tyr Ala Gly Tyr Ile Glu Ser Gly Val Glu 
        355                 360                 365             


Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Leu Lys Ile Asn 
    370                 375                 380                 


Gly Ser Gly Asp Phe Leu Asp Lys Ile Asp Cys Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         


Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 


Ala Glu Tyr Lys Ala Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 


Ile Leu Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     


Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Pro Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Asn Glu Gln Gly Glu Ala Lys Phe Phe Asp Thr Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 


Asp Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     


Val Asn Leu Thr Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ser Ser 
            580                 585                 590         


Leu Gly Thr Tyr His Asp Leu Arg Lys Ile Leu Asp Lys Ser Phe Leu 
        595                 600                 605             


Asp Asp Lys Ala Asn Glu Lys Thr Ile Glu Asp Ile Ile Gln Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr 
625                 630                 635                 640 


Ser Asp Ile Phe Thr Lys Ala Gln Leu Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Asn Lys Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
        675                 680                 685             


Tyr Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser 
    690                 695                 700                 


Phe Lys Glu Glu Ile Ala Arg Ala Gln Ile Ile Gly Asp Val Asp Asp 
705                 710                 715                 720 


Ile Ala Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly His Asn Pro Ala Asn Ile Ile Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Asp Lys Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Leu Leu 
    770                 775                 780                 


Gln Asp Ser Leu Lys Asn Leu Asp Asn Pro Val Asn Ile Lys Asn Val 
785                 790                 795                 800 


Glu Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln 
                805                 810                 815     


Asn Gly Lys Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asn Asn Leu 
            820                 825                 830         


Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp 
        835                 840                 845             


Asn Ser Leu Asp Asn Arg Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asp Val Pro Ser Ile Glu Val Val His Glu Met Lys Ser 
865                 870                 875                 880 


Phe Trp Ser Lys Leu Leu Ser Val Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Glu Arg Phe Asn Thr Glu Phe Asp Gly 
    930                 935                 940                 


Ala Gln Arg Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn 
945                 950                 955                 960 


Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Asn Ala Leu Leu Leu Lys Tyr  Pro Gln Leu Glu Pro  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Glu Tyr Pro Lys Tyr  Asn Ser Tyr Arg Ser  Arg Lys Ser 
    1010                 1015                 1020             


Ala Thr  Glu Lys Phe Leu Phe  Tyr Ser Asn Ile Leu  Arg Phe Phe 
    1025                 1030                 1035             


Lys Lys  Glu Asp Ile Gln Thr  Asn Glu Asp Gly Glu  Ile Ala Trp 
    1040                 1045                 1050             


Asn Lys  Glu Lys His Ile Lys  Ile Leu Arg Lys Val  Leu Ser Tyr 
    1055                 1060                 1065             


Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu Glu Gln  Thr Gly Gly 
    1070                 1075                 1080             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly Glu Ser  Asp Lys Leu 
    1085                 1090                 1095             


Ile Pro  Arg Lys Thr Lys Asn  Ser Tyr Trp Asn Pro  Lys Lys Tyr 
    1100                 1105                 1110             


Gly Gly  Phe Asp Ser Pro Val  Val Ala Tyr Ser Ile  Leu Val Phe 
    1115                 1120                 1125             


Ala Asp  Val Glu Lys Gly Lys  Ser Lys Lys Leu Arg  Lys Val Gln 
    1130                 1135                 1140             


Asp Met  Val Gly Ile Thr Ile  Met Glu Lys Lys Arg  Phe Glu Lys 
    1145                 1150                 1155             


Asn Pro  Val Asp Phe Leu Glu  Gln Arg Gly Tyr Arg  Asn Val Arg 
    1160                 1165                 1170             


Leu Glu  Lys Ile Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu 
    1175                 1180                 1185             


Glu Asn  Lys Arg Arg Arg Leu  Leu Ala Ser Ala Arg  Glu Leu Gln 
    1190                 1195                 1200             


Lys Gly  Asn Glu Leu Val Ile  Pro Gln Arg Phe Thr  Thr Leu Leu 
    1205                 1210                 1215             


Tyr His  Ser Tyr Gln Ile Glu  Lys Asn Tyr Glu Pro  Glu His Arg 
    1220                 1225                 1230             


Glu Tyr  Val Glu Lys His Lys  Asp Glu Phe Lys Glu  Leu Leu Glu 
    1235                 1240                 1245             


Tyr Ile  Ser Val Phe Ser Arg  Lys Tyr Val Leu Ala  Asp Asn Asn 
    1250                 1255                 1260             


Leu Thr  Lys Ile Glu Met Leu  Phe Ser Lys Asn Lys  Asp Ala Glu 
    1265                 1270                 1275             


Val Ser  Ser Leu Ala Lys Ser  Phe Ile Ser Leu Leu  Thr Phe Thr 
    1280                 1285                 1290             


Ala Phe  Gly Ala Pro Ala Ala  Phe Asn Phe Phe Gly  Glu Asn Ile 
    1295                 1300                 1305             


Asp Arg  Lys Arg Tyr Thr Ser  Val Thr Glu Cys Leu  Asn Ala Thr 
    1310                 1315                 1320             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1325                 1330                 1335             


Leu Ser  Lys Leu Gly Glu Asp  
    1340                 1345 


<210>  894
<211>  1346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  894

Met Asn Gln Thr Lys Asn Tyr Asp Ile Gly Leu Asp Ile Gly Val Gly 
1               5                   10                  15      


Ser Val Gly Trp Thr Ile Thr Asp Ser Gln Ser Gln Lys Val Leu Lys 
            20                  25                  30          


Val Lys Gly Lys Gln Gly Ile Gly Val His Leu Phe Asn Glu Gly Gln 
        35                  40                  45              


Thr Ala Glu Glu Arg Arg Gln Phe Arg Thr Asn Arg Arg Arg Leu Lys 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Arg Leu Leu Arg Glu Leu Phe Asp Glu Pro 
65                  70                  75                  80  


Ile Ser Ser Ile Asp Pro Glu Phe Phe Ala Arg Leu Lys Tyr Phe Asn 
                85                  90                  95      


Glu Ser Ser Lys Asp Pro Lys Asn Lys Gly Tyr Gln Pro Gln Leu His 
            100                 105                 110         


Asp Asp Leu Lys Asp Val Asp Phe Tyr Arg Gln Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg His Ala Leu Met Thr Gln Lys Glu Lys Phe Asp Ile Arg 
    130                 135                 140                 


Asp Ile Tyr Leu Ala Ile His His Ile Val Lys Tyr Arg Gly His Phe 
145                 150                 155                 160 


Leu Met Asn Gly His Ser Ser Ser Phe Ser Val Ala Gln Thr Asp Trp 
                165                 170                 175     


Lys Ala Asp Leu Thr Lys Leu Phe Glu Leu Ile Gln Glu Thr Arg Thr 
            180                 185                 190         


Asn Leu Asp Phe Ser Phe Asp Leu Thr Glu Ile Asp Thr Ile Lys Ser 
        195                 200                 205             


Leu Leu Phe Asp Lys Asn Leu Ser Arg Asn Asp Arg Gln Lys Glu Ile 
    210                 215                 220                 


Val Gly Leu Ile Asn Asn Asn Ser Gln Leu Asp Lys Lys Asp Lys Ala 
225                 230                 235                 240 


Leu Thr Lys Gln Leu Val Glu Ser Val Val Lys Gly Ile Val Gly Met 
                245                 250                 255     


Lys Thr Arg Thr Asp Ile Leu Leu Gly Tyr Glu Val Asp Asn Lys Glu 
            260                 265                 270         


Gln Ser Lys Ala Trp Asn Phe Ser Ile Asp Glu Leu Asp Asp Gln Met 
        275                 280                 285             


Ala Thr Ile Asp Pro Ala Leu Ser Gln Asn Ser Gln Ala His Asp Ile 
    290                 295                 300                 


Ile Thr Leu Ile Gln Lys Leu Tyr Met Thr Val Gln Leu Ala Gln Ile 
305                 310                 315                 320 


Val Pro Glu Gly Gln Ser Val Ser Glu Met Met Ile Asn Arg Tyr Asn 
                325                 330                 335     


Leu Tyr Ala Glu Gln Leu Gln Gln Leu Lys Asp Tyr Lys Lys Ile Asn 
            340                 345                 350         


Leu Glu Thr Gly Leu Leu Leu Arg Glu Ala Tyr Asp Glu Leu Met Thr 
        355                 360                 365             


Lys Ala Leu Thr Ala Ala Pro Gly Lys Thr Ile His Asp Thr Phe Tyr 
    370                 375                 380                 


Asn Lys Val Lys Lys Ile Leu Lys Asp Gln Asp Asn Lys Gln Ala Leu 
385                 390                 395                 400 


Ser Ile Lys Gln Ala Ile Glu Asp Ser Asn Tyr Leu Ile Pro Leu Arg 
                405                 410                 415     


Ile Lys Ser Asn Gly Val Ile Pro Tyr Gln Leu His Gln Arg Glu Leu 
            420                 425                 430         


Asp Ala Ile Ile Glu Asn Gln Lys Glu Phe Tyr Pro Phe Leu Ala Thr 
        435                 440                 445             


Pro Asn Pro Val Asp Gly His Gln Glu Ala Leu Pro Tyr Lys Leu Asp 
    450                 455                 460                 


Glu Leu Ile Gly Phe Lys Val Pro Tyr Tyr Val Gly Pro Met Val Asp 
465                 470                 475                 480 


Pro Glu Val Asp Ser Asp Gln Asn Phe Ala Trp Met Val Arg Lys Glu 
                485                 490                 495     


Lys Gly Glu Ile Thr Pro Trp Asn Phe His Asn Lys Val Asp Ile Thr 
            500                 505                 510         


Gln Ser Ala Asp Asn Phe Ile Lys Arg Met Lys Thr Thr Asp Thr Tyr 
        515                 520                 525             


Leu Ile Gly Lys Asp Val Leu Pro Gln Asn Ser Leu Leu Tyr Gln Arg 
    530                 535                 540                 


Tyr Thr Val Leu Asn Glu Leu Asn Gly Ile Lys Val Ala Asn Lys Lys 
545                 550                 555                 560 


Leu Thr Ile Glu Gln Lys Gln Arg Leu Tyr Asp Gln Leu Phe Lys Lys 
                565                 570                 575     


Lys Lys Thr Val Lys Lys Gln Asp Ile Leu Asn Asn Leu Val Ala Ala 
            580                 585                 590         


Gly Glu Ile His Glu Asp Asp Asn Leu Thr Gly Leu Ser Asn Gly Thr 
        595                 600                 605             


Thr Phe Asn Asn Asn Leu Gly Ser Tyr Ile Asp Tyr Cys Lys Ile Ile 
    610                 615                 620                 


Gly Gln Asp Lys Leu Glu Asp Ala Ser Lys Gln Ala Asp Ile Glu Arg 
625                 630                 635                 640 


Ile Ile Glu Trp Ser Thr Thr Phe Glu Asp Ala Asn Ile Phe Leu Thr 
                645                 650                 655     


Lys Leu Ala Glu Ile Asn Trp Leu Thr Glu Asp Gln Arg Gln Lys Leu 
            660                 665                 670         


Ser Gln Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Lys Thr Leu Leu 
        675                 680                 685             


Thr Asp Ile Lys Asp Asn Lys Asp Asn Ser Ile Ile Asp Asn Leu Met 
    690                 695                 700                 


Ser Thr Lys Lys Val Phe Met Gln Ile Ile Asn Asp Thr Thr Ile Lys 
705                 710                 715                 720 


Gln Lys Ile Asp Asp Tyr Asn Gln Tyr His Leu Asn His Ala Asp Leu 
                725                 730                 735     


Lys Asp Thr Ile Ala Asn Leu Phe Thr Ser Pro Gln Asn Lys Arg Ala 
            740                 745                 750         


Ile Asn Gln Thr Leu Leu Val Ile Glu Asp Ile Ile Asn Ala Val Gly 
        755                 760                 765             


His Lys Pro Asn Arg Ile Phe Ile Glu Ser Ala Lys Asp Asn Arg Asn 
    770                 775                 780                 


Ala Lys Asn Gln Leu Thr Gln Ser Arg Leu Lys Arg Ile Val Lys Gln 
785                 790                 795                 800 


Phe Glu Asp Asn Lys Gln His Val Asp Gln Ser Val Tyr Glu Glu Leu 
                805                 810                 815     


Lys Gln Leu Asp Asp Gly Ser His Ala Ser Ile Asp Asp Arg Leu Leu 
            820                 825                 830         


Leu Tyr Phe Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Pro Ile 
        835                 840                 845             


Asp Ile Ser Gln Leu Ser Thr Ser Gln Tyr His Ile Asp His Ile Leu 
    850                 855                 860                 


Pro Gln Ala Lys Val Met Asp Asn Ala Leu Asp Asn Arg Val Leu Val 
865                 870                 875                 880 


Ser Ser Lys Ala Asn Thr Glu Lys Ser Asp Arg Phe Leu Ser Glu Ile 
                885                 890                 895     


Pro Gln Phe Gln Gln Arg Ser Leu Trp Ala Glu Leu Glu Arg Met Gly 
            900                 905                 910         


Phe Leu Thr Lys Ala Lys Lys Glu Arg Leu Leu Met Thr Ser Asp Glu 
        915                 920                 925             


Phe Lys Gln Asn Ser Val Arg Phe Ile Ala Arg Gln Leu Val Glu Thr 
    930                 935                 940                 


Arg Gln Ile Ile Lys Leu Val Asn Gln Leu Ile Ile Gln Glu His Pro 
945                 950                 955                 960 


Glu Val Lys Leu Val Asn Ile Lys Ala Asp Leu Thr Asn Ala Phe Arg 
                965                 970                 975     


Lys Lys Phe Asn Phe Val Lys Asn Arg Asn Ala Asn Asp Tyr His His 
            980                 985                 990         


Ala Phe Asp Ala Tyr Leu Thr Ser  Leu Ile Gly Ser His  Leu Leu Lys 
        995                 1000                 1005             


Ser His  Pro Lys Trp Glu Pro  Leu Phe Val Tyr Ser  Asp Tyr Asp 
    1010                 1015                 1020             


Lys Ile  Lys Phe Asp Asn Ala  Pro Gln Gly Val Asn  Ile Met Lys 
    1025                 1030                 1035             


Glu Phe  Glu Gln Ser Lys Leu  Ala Asp Glu Asn Gly  Glu Ile Ile 
    1040                 1045                 1050             


Trp Asn  Lys Asp Glu Met Met  Ala Tyr Leu Glu Arg  Leu Tyr Asn 
    1055                 1060                 1065             


Ser Arg  Gln Ile Leu Ile Thr  Lys Lys Val Tyr Ala  Arg His Gly 
    1070                 1075                 1080             


Asn Leu  Phe Lys Gln Thr Ile  Phe Lys Ala Gly Glu  Ser Asp Asn 
    1085                 1090                 1095             


Leu Ile  Pro Val Lys Asn Asn  Arg Ser Asp Ile Ala  Ile Tyr Gly 
    1100                 1105                 1110             


Gly Tyr  Lys Ser Lys Gln Val  Ser Tyr Tyr Ala Leu  Ile Ser Leu 
    1115                 1120                 1125             


His Asn  Lys Lys Arg Gln Lys  Arg Leu Val Ala Val  Tyr Leu Ile 
    1130                 1135                 1140             


Asp Ile  Gln Lys Leu Gln Asn  Leu Asn Glu Ser Asp  Tyr Phe Lys 
    1145                 1150                 1155             


Thr Leu  His Thr Ile Ile Lys  Glu Arg His Asn Ile  Lys Pro Glu 
    1160                 1165                 1170             


Phe Asp  Ile Leu Ala Asp Lys  Ile Tyr Gln Asn Gln  Leu Ile Ile 
    1175                 1180                 1185             


Asp Asn  Lys Asn Gly Ser Asn  Arg Pro Phe Arg Ile  Glu Gly Asp 
    1190                 1195                 1200             


Phe Lys  Asn Asp Arg Gln Leu  Tyr Leu Pro Leu Gln  Tyr Gln Arg 
    1205                 1210                 1215             


Trp Leu  Ala Ser Lys Glu Pro  Thr Asp Glu Lys Leu  Asn Arg Ile 
    1220                 1225                 1230             


Phe Asp  His Ile Cys Glu Gln  Leu Ala Asp Tyr Phe  Pro Leu Phe 
    1235                 1240                 1245             


Ala Gly  Asn Ala Cys Asp Lys  Leu Leu Asp Asn Arg  Asp Lys Phe 
    1250                 1255                 1260             


Tyr Gln  Leu Ser Ile Lys Ser  Glu Phe Asn Asp Lys  Gly Lys Leu 
    1265                 1270                 1275             


Ile Ala  Glu Gly Lys Leu Ser  Val Ile Asp Lys Leu  Leu Val Ala 
    1280                 1285                 1290             


Met His  Ala Asp Ser Arg Asn  Ser Asn Leu Ala Val  Ile Gly Leu 
    1295                 1300                 1305             


Ser Ser  Ser Trp Gly Arg Phe  Lys Leu His Gly Gly  Asn Glu Ala 
    1310                 1315                 1320             


Ile Gly  Thr Ser Val Gln Leu  Ile His Gln Ser Pro  Thr Gly Leu 
    1325                 1330                 1335             


Phe Lys  Arg Ile Gln Arg Phe  Asn 
    1340                 1345     


<210>  895
<211>  1346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  895

Met Leu Ser Ala Gly Val Met Leu Gly Asp Val Ser Ala Leu Lys Ser 
1               5                   10                  15      


Ser Arg Asn Ser Leu Phe Gln Lys Ser Lys Lys Leu Ile Glu Asn Ser 
            20                  25                  30          


Ser Ser Asp Leu Thr Lys Val Asp Tyr Ser Lys Met Arg Glu Ile Leu 
        35                  40                  45              


Leu Ser Ile Arg Asn Ile Ala Ile Arg Phe Phe Asn Asn Glu Asp Phe 
    50                  55                  60                  


Lys Glu Ser Asp Tyr Tyr Lys Glu Phe Pro Thr Ile Trp His Leu Arg 
65                  70                  75                  80  


Lys Asn Leu Met Glu Ser Asp Lys Lys Ala Asp Ile Arg Leu Val Tyr 
                85                  90                  95      


Leu Ala Leu His Asn Ile Val Lys Tyr Arg Gly Asn Phe Leu Gln Glu 
            100                 105                 110         


Asn Asn Lys Asp Leu Arg Ala Ala Asn Ala Asn Ala Gly Ala Ala Ala 
        115                 120                 125             


Ile Asp Leu Ser Asn Ala Leu Glu Glu Tyr Phe Ser Leu His Glu Asp 
    130                 135                 140                 


Glu Ser Ala Leu Thr Cys Val Pro Asn Val Ala Asp Leu Gln Met Thr 
145                 150                 155                 160 


Leu Glu Gln Pro Gly Leu Arg Asn Gly Glu Arg Leu Asp Arg Ile Val 
                165                 170                 175     


Ala Ala Leu Asn Met Pro Asp Arg Ala Arg Ala Lys Leu Phe Ala Lys 
            180                 185                 190         


Ala Cys Ile Gly Gln Lys Val Glu Phe Ser Lys Leu Ala Leu Gly Met 
        195                 200                 205             


Glu Lys Gln Glu Gly Thr Asn Ala Tyr Ile Trp Asp Asp Asp Lys Tyr 
    210                 215                 220                 


Asp Glu Leu Val Ala Leu Cys Pro Glu Asp Phe Phe Pro Val Leu Glu 
225                 230                 235                 240 


Thr Leu Lys Asn Ala Tyr Ser Ala Tyr Val Leu Ser Asp Leu Leu Ala 
                245                 250                 255     


Gly Lys Leu Ser Ile Ser Ala Ser Leu Ile Asp Ser Tyr Glu Gln His 
            260                 265                 270         


Gln Asn Asp Leu Lys Ile Val Lys Glu Leu Val Arg Glu Tyr Leu Gly 
        275                 280                 285             


Gln Lys Glu Tyr Gly Arg Leu Phe Arg Gly Pro Lys Phe Ala Asn Gly 
    290                 295                 300                 


Asp Tyr Asn Phe Asn Lys Val Ala Lys Pro Ser Tyr Thr Ser Tyr Ile 
305                 310                 315                 320 


Leu Gly Glu Ser Leu Cys Asn Lys Gln Gly Thr Thr Arg Glu Glu Leu 
                325                 330                 335     


Val Lys Glu Leu Lys Arg Val Phe Asp Asp Cys Ser Ala Ile Gln Ala 
            340                 345                 350         


Asp Lys Arg Tyr Gln Lys Ile Lys Gln Arg Leu Glu Asp Asp Pro Lys 
        355                 360                 365             


Phe Leu Ile Lys Gln Lys Thr Arg Ala Asn Gly Ala Ile Pro Tyr Gln 
    370                 375                 380                 


Leu His Leu Glu Glu Met His Gln Ile Ile Glu Lys Gln Gly Lys Tyr 
385                 390                 395                 400 


Tyr Pro Phe Leu Val Glu Gln Gln Asp Leu Ile Glu Lys Leu Val Ser 
                405                 410                 415     


Ser Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Gly Gly His Asp Pro 
            420                 425                 430         


Asn His Pro Tyr Pro Thr Asn Pro Ile Asp Pro Lys Arg Lys Phe Ala 
        435                 440                 445             


Trp Ser Val Arg Lys Pro Gly Met Glu His Glu Lys Ala Tyr Pro Trp 
    450                 455                 460                 


Asn Val Asp Glu Val Ile Asp Ser Glu Glu Thr Ala Glu Arg Phe Ile 
465                 470                 475                 480 


Arg Arg Met Thr Gly Thr Cys Thr Tyr Leu Tyr Gly Glu Pro Val Val 
                485                 490                 495     


Pro Arg Cys Ser Leu Leu Tyr Glu Glu Phe Cys Val Leu Asn Glu Leu 
            500                 505                 510         


Asn Gly Ala Arg Trp Gly Glu Gly Asn Gly Asp Thr His Arg Phe Asp 
        515                 520                 525             


Ser Ser Asp Arg Thr Glu Ile Leu Glu Gln Leu Phe Lys Lys Arg Lys 
    530                 535                 540                 


Arg Val Thr His Lys Ala Ile Ala Thr Trp Leu Glu Ile Asn His Gly 
545                 550                 555                 560 


Val Thr Asn Val Lys Ile Ser Gly Thr Gln Ala Glu Asn Ala Phe Glu 
                565                 570                 575     


Ser Lys Leu Ser Ser Tyr Asn Asp Phe Cys Asn Leu Leu Gly Val Asp 
            580                 585                 590         


Gly Leu Glu Asp Val Cys Pro Leu Thr Lys Asp Asp Ile Glu Gln Ile 
        595                 600                 605             


Ile Leu Trp Val Thr Val Phe Glu Asp Lys Lys Leu Leu Leu Gln Lys 
    610                 615                 620                 


Leu Gln Arg Ala Phe Gly Asp Lys Leu Ser Glu Lys Gln Ile Ala Ser 
625                 630                 635                 640 


Ile Met Arg Lys Pro Tyr Ala Gly Trp Gly Arg Leu Ser Ser Lys Leu 
                645                 650                 655     


Leu Thr Gly Ile Lys Arg Glu Thr Pro Phe Gly Arg Ile Ser Ile Leu 
            660                 665                 670         


Asp Val Met Arg Asp Gly Asp Pro Arg Pro Gly His His Leu Asp Ala 
        675                 680                 685             


Met Asn Phe Met Glu Ile Leu Arg Glu Lys Asp Phe Gly Phe Gln Asp 
    690                 695                 700                 


Arg Ile Asp Glu Ile Asn Lys Glu Arg Thr Lys Glu Ser Gly Leu Leu 
705                 710                 715                 720 


Leu Ser Val Glu Asp Leu Pro Gly Ser Pro Ala Leu Arg Arg Ser Val 
                725                 730                 735     


Asn Gln Ala Met His Ile Leu Lys Glu Ile Val Ser Ile Ala Gly Lys 
            740                 745                 750         


Glu Pro Ser Arg Ile Val Ile Glu Val Thr Arg Asp Pro Asp Glu Glu 
        755                 760                 765             


Lys Arg Gly His Arg Thr Asp Thr Arg Tyr Lys Gln Leu Ser Glu Ala 
    770                 775                 780                 


Leu Ala Ala Ile Lys Thr Asp Ile Val Gln Phe Asp Ser Ser Leu Leu 
785                 790                 795                 800 


Ala Glu Leu Lys Glu Arg Lys Asp Ser Leu Asp Ser Glu Arg Leu Met 
                805                 810                 815     


Leu Tyr Phe Gln Gln Cys Gly Lys Ser Leu Tyr Ser Gly Lys Pro Leu 
            820                 825                 830         


Asp Ile Asn Arg Leu Ser Glu Tyr Gln Val Asp His Ile Ile Pro Gln 
        835                 840                 845             


Cys Tyr Ile Lys Asp Asp Ser Leu Asp Asn Lys Ala Leu Val Phe Ala 
    850                 855                 860                 


Ser Glu Asn Gln Ile Lys Leu Asp Ser Leu Leu Leu Asp Lys Ser Ile 
865                 870                 875                 880 


Ile Arg Ser Gln Lys Gly Arg Trp Gln Glu Leu His Arg Ala Gly Leu 
                885                 890                 895     


Ile Ser Asn Lys Lys Phe Arg Asn Leu Thr Cys Thr Glu Ile Ser Asp 
            900                 905                 910         


Arg Met Met Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln 
        915                 920                 925             


Ile Val Lys Phe Val Arg Gln Met Cys Glu Gln Arg Tyr Glu Ser Ser 
    930                 935                 940                 


Glu Val Ile Ser Leu Arg Ala Ser Leu Ser His Asn Leu Arg Asp Arg 
945                 950                 955                 960 


Cys Gly Phe Ile Lys Cys Arg Glu Leu Asn Asp Tyr His His Ala His 
                965                 970                 975     


Asp Ala Tyr Leu Ala Cys Gln Ile Ala Arg Phe Ile Gly Ile Arg Tyr 
            980                 985                 990         


Pro Arg Trp Gln Asp Gly Ile Ser  Arg Glu Phe Ile Asn  Lys Tyr Ile 
        995                 1000                 1005             


Lys Gln  Leu Val Lys Lys Gly  Lys Ser Ser Gly Lys  Phe Phe Ala 
    1010                 1015                 1020             


Gly Lys  Ser Gly Phe Ile Ile  Asp Ser Phe Met His  Asp Gly Phe 
    1025                 1030                 1035             


Asp Arg  Glu Thr Gly Glu Ile  Phe Arg Asp Gly Trp  Asp Ala Thr 
    1040                 1045                 1050             


Phe Glu  Ile Thr Arg Met Arg  Arg Val Leu Gly Tyr  Lys Asp Cys 
    1055                 1060                 1065             


Phe Ile  Thr Arg Met Pro Glu  Glu Gln Thr Gly Ala  Tyr Trp Glu 
    1070                 1075                 1080             


Glu Thr  Ile Tyr Ser Pro Leu  Asp Ala Lys Asn Gly  Lys Asn Leu 
    1085                 1090                 1095             


Gln Ile  Pro Leu Lys Gln Ser  Gly Lys Glu Gly Tyr  Leu Asp Pro 
    1100                 1105                 1110             


Lys Lys  Tyr Gly Gly Thr Asn  Thr Ala Phe Asn Ala  Tyr Phe Phe 
    1115                 1120                 1125             


Ala Phe  Val Ala Lys Asp Ala  Arg Gly Arg Asp Lys  Tyr Phe Phe 
    1130                 1135                 1140             


Glu Gly  Val Pro Val Tyr Leu  Ala Lys Gln Leu Asn  Glu Gln Pro 
    1145                 1150                 1155             


Gln Ile  Leu Val Asp Phe Ala  Asn Ser Ile Ala Ala  Thr Lys Lys 
    1160                 1165                 1170             


Cys Ser  Thr Ala Lys Ile Leu  Arg Ser Lys Val Pro  Leu Arg Gln 
    1175                 1180                 1185             


Lys Phe  Leu Leu Asp Gly Thr  Ser Phe Tyr Leu Gly  Gly Arg Thr 
    1190                 1195                 1200             


Gly Asn  Lys Asn Val Ile Leu  Ser Gly Val Glu Phe  Ala Cys Gly 
    1205                 1210                 1215             


Ile Asp  Met Ala Lys Met Ile  Asp Thr Ala Ile Lys  His Pro Glu 
    1220                 1225                 1230             


Glu Leu  Ser Gln Lys Glu Leu  Ile Ser Ile Ile Gln  Met Leu Ala 
    1235                 1240                 1245             


Asp Thr  Ile Glu Arg Asn Ser  Asp Arg Gln Ala Ser  Leu Ile Asn 
    1250                 1255                 1260             


Phe Arg  Arg Phe Asp Asp Lys  Leu Arg Asn Ile Asp  Ala Ile Ala 
    1265                 1270                 1275             


Ala Ser  Arg Ile Ile Lys Asn  Leu Leu Asn Ser Met  Asn Gly Val 
    1280                 1285                 1290             


Lys Gln  Ser Cys Asp Leu Ser  Asp Leu Gly Gly Ser  Lys Gln Ser 
    1295                 1300                 1305             


Gly Phe  Met Gln Ser Asn Ile  Ala Thr Leu Phe Glu  Arg Ile Thr 
    1310                 1315                 1320             


Trp Cys  Tyr Ser Ser Ile Thr  Gly Met Phe Glu Gln  Arg Val Ser 
    1325                 1330                 1335             


Phe Glu  Asp Leu Lys Asn Gly  Ile 
    1340                 1345     


<210>  896
<211>  1346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  896

Met Lys Lys Arg Asn Arg Glu Glu Tyr Tyr Val Gly Leu Asp Ile Gly 
1               5                   10                  15      


Thr Ser Ser Val Gly Trp Ala Val Ala Thr Pro Asp Tyr Arg Ile Pro 
            20                  25                  30          


Lys Phe Arg Gly His Lys Met Trp Gly Ser Arg Leu Phe Ala Glu Gly 
        35                  40                  45              


Lys Arg Ala Ala Glu Arg Arg Gln Phe Arg Thr Asn Arg Arg Arg Leu 
    50                  55                  60                  


Ala Arg Arg Arg Asp Arg Leu Gln Ile Leu Glu Glu Leu Phe Ala Glu 
65                  70                  75                  80  


Glu Met Ala Lys Val Asp Asn Thr Phe Phe Met Arg Leu Lys Glu Ser 
                85                  90                  95      


Lys Phe Phe Leu Glu Asp Lys Thr Val Lys Glu Lys His Ile Leu Phe 
            100                 105                 110         


Ser Thr Asp Lys Phe Asp Ala Asp Arg Phe Tyr Lys Asp Asn Pro Thr 
        115                 120                 125             


Ile Tyr His Val Arg Lys Arg Leu Met Glu Glu Glu Pro Gln Asp Ile 
    130                 135                 140                 


Arg Glu Leu Phe Leu Ala Val His His Ile Leu Lys Tyr Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Ala Gly Ser Leu Glu Gln Asn Gly Asp Ser Ile Glu Glu 
                165                 170                 175     


Leu Val Arg Thr Leu Phe Gly Lys Gln Gly Leu Ala Ile Leu Asp Asp 
            180                 185                 190         


Thr Ile Asp Ser Asn Glu Met Val Asn Ile Leu Lys Asn Asn Glu Lys 
        195                 200                 205             


Thr Lys Ser Asp Arg Lys Lys Ala Val Gln Glu Leu Trp Asn Pro Leu 
    210                 215                 220                 


Lys Asp Lys Val Lys Lys Ala Gln Ala Ala Glu Met Ala Gly Val Val 
225                 230                 235                 240 


Leu Gly Leu Asn Ser Asp Ile Ser Lys Leu Phe Asp Asn Ser Asp Tyr 
                245                 250                 255     


Lys Asp Leu Lys Ile Asn Phe Ser Ser Gly Asp Tyr Asp Glu Lys Arg 
            260                 265                 270         


Asp Asp Tyr Ala Asn Ser Ile Gly Asp Asp Ile Val Leu Leu Asp Thr 
        275                 280                 285             


Met Lys Ala Leu Tyr Asp Thr Leu Ile Leu Ser Gln Ile Leu Gln Gly 
    290                 295                 300                 


Lys Gln Ser Ile Ser Glu Ala Lys Val Glu Asp Tyr Asn Thr His Lys 
305                 310                 315                 320 


Glu Gln Leu Arg Ala Leu Lys Ala Ile Leu Lys Pro His Lys Arg Leu 
                325                 330                 335     


Tyr Asn Glu Met Phe Arg Glu Asn Asn Lys Lys Asp Leu Ser Asn Tyr 
            340                 345                 350         


Val Ala Tyr Ile Ser Ser Tyr Ser Glu Gly Lys Arg Val Asn Arg Glu 
        355                 360                 365             


Gly Phe Tyr Lys Tyr Val Gln Ala Val Leu Lys Gln Val Asp Asp Ser 
    370                 375                 380                 


Asp Ser Lys Gln Ala Ile Leu Glu Ala Ile Arg Leu Glu Thr Phe Leu 
385                 390                 395                 400 


Pro Leu Leu Arg Val Lys Asp Asn Ala Val Ile Pro His Gln Ile His 
                405                 410                 415     


Gln Ala Glu Leu Glu Ala Ile Leu Glu Thr Ala Arg Lys Lys Tyr Lys 
            420                 425                 430         


Phe Leu Asn Asp Val Ser Glu Gly Val Ser Val Lys Asp Lys Ile Ile 
        435                 440                 445             


Gln Leu Phe Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Pro 
    450                 455                 460                 


His Ser Gly Glu Thr Val Ala Asp Arg Lys Gln Gly Thr Gly Trp Val 
465                 470                 475                 480 


Val Arg Lys Gln Arg Gly Lys Val Tyr Pro Trp Asn Phe Ala Asp Met 
                485                 490                 495     


Val Asp Val Lys Ala Ser Ala Glu Ala Phe Ile Thr Asn Leu Thr Asn 
            500                 505                 510         


Lys Cys Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Arg Ser Leu 
        515                 520                 525             


Leu Tyr Ser Glu Phe Met Leu Leu Asn Glu Leu Asn Met Ile Lys Tyr 
    530                 535                 540                 


Asp Gly His Glu Leu Pro Val Glu Ile Lys Arg Leu Tyr Ile Glu Gln 
545                 550                 555                 560 


Val Phe Lys Gln Glu His Lys Lys Gln Thr Lys Gly Arg Val Leu Asp 
                565                 570                 575     


Phe Leu Lys Arg Asn Gly Leu Ile Thr Gly Ser Ala Thr Ala Val Ile 
            580                 585                 590         


Thr Gly Met Asp Asp Ser Ile Lys Gly Asp Leu Gln Ser Tyr Arg Asp 
        595                 600                 605             


Met Val Arg Ile Phe Gly Thr Gly Phe Asp His Ser Met Ala Glu Arg 
    610                 615                 620                 


Ile Ile Arg Trp Val Thr Leu Phe Gly Glu Ala Lys Asp Ile Leu Glu 
625                 630                 635                 640 


Asn Lys Ile Arg Glu Val Tyr Gly Asn Ser Ile Ser Asp Ser Gln Met 
                645                 650                 655     


Arg Ala Ile Lys Lys Leu Lys Tyr Ala Asp Trp Gly Arg Leu Ser Gln 
            660                 665                 670         


Ala Phe Phe Ile Lys Ile Ile Gly Cys Asp Lys Ser Gly Val Glu Arg 
        675                 680                 685             


Asn Ile Ile Thr Cys Leu Arg Glu Ser Ser Gln Asn Leu Met Glu Ile 
    690                 695                 700                 


Leu Ser Asp Gln Gly Thr Tyr Ile Lys Gln Ile Lys Ala Trp Asn His 
705                 710                 715                 720 


Gln His Thr Thr Asn Met Met Asp Thr Leu Asp Tyr Gln Leu Val Asp 
                725                 730                 735     


Asp Leu Tyr Val Ala Pro Ser Val Lys Arg Ser Ile Trp Gln Ser Leu 
            740                 745                 750         


Cys Ile Val Glu Glu Leu Val Lys Ile Met Gly Gln Ala Pro Lys Lys 
        755                 760                 765             


Ile Phe Val Glu Val Thr Arg Thr Asn Gln Ala Glu Lys Thr Lys Lys 
    770                 775                 780                 


Asp Ser Arg Lys Lys Arg Leu Val Glu Leu Tyr Thr Ala Ile Gly Lys 
785                 790                 795                 800 


Glu Gly Ala Ala Trp Lys Lys Glu Ile Glu Lys His Gln Ala Gly Asp 
                805                 810                 815     


Phe Arg Arg Lys Lys Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Cys 
            820                 825                 830         


Met Tyr Ser His Glu Pro Ile Ala Met Asp Ala Leu Phe Thr Ser Glu 
        835                 840                 845             


Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Leu Thr Lys Asp Asp Ser 
    850                 855                 860                 


Trp Asp Asn Leu Val Leu Val Lys Ser Gln Leu Asn Lys Glu Lys Ser 
865                 870                 875                 880 


Asp Gln Tyr Pro Leu Arg Pro Ala Ile Arg Glu Lys Asn Lys Ala Tyr 
                885                 890                 895     


Trp Gln Tyr Leu Cys Arg Ala Gly Phe Ile Ser Glu Lys Lys Tyr Glu 
            900                 905                 910         


Arg Leu Met Arg Thr Thr Glu Leu Thr Ala Glu Glu Leu Ser Gly Phe 
        915                 920                 925             


Ile Ala Arg Gln Leu Val Glu Thr Ser Gln Ala Thr Lys Ala Val Ile 
    930                 935                 940                 


Gly Leu Leu Glu Arg Ile Tyr Lys His Thr Asp Ile Cys Phe Val Lys 
945                 950                 955                 960 


Ala Glu Thr Val Ser Gln Phe Arg His Gln His Asn Phe Ile Lys Ile 
                965                 970                 975     


Arg Glu Leu Asn Asn Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile 
            980                 985                 990         


Val Val Gly Asn Val Tyr His Glu  Lys Phe Thr Lys Asn  Pro Arg Glu 
        995                 1000                 1005             


Phe Ile  Ala Arg Ser Thr Glu  Arg Arg Pro Tyr Asn  Leu Asn Arg 
    1010                 1015                 1020             


Met Tyr  Asp Phe Asp Leu Leu  Lys Gln Gly Arg Val  Ile Trp Gln 
    1025                 1030                 1035             


Val Pro  Lys Ser Leu Glu Thr  Val Gln Ala Met Met  Lys Ser Asn 
    1040                 1045                 1050             


Asp Val  Arg Ile Thr Lys Lys  Leu Leu Val Gln Lys  Gly Glu Leu 
    1055                 1060                 1065             


Tyr Asp  Ala Thr Ile Thr Lys  Ala His Lys Thr Lys  Glu Gly Met 
    1070                 1075                 1080             


Tyr Phe  Pro Leu Lys Gly Ala  Asp Ala Arg Leu Arg  Asp Val His 
    1085                 1090                 1095             


Arg Tyr  Gly Gly Tyr Glu Lys  Ile Lys Ile Ala Tyr  Tyr Ser Val 
    1100                 1105                 1110             


Phe Ser  Cys Cys Glu Lys Ala  Ser Asp Thr Ile His  Thr Leu Ile 
    1115                 1120                 1125             


Ile Pro  Ile Pro Val Tyr Ala  Ala Gly Leu Leu Gln  Thr Glu Ala 
    1130                 1135                 1140             


Asp Leu  Thr Ala Tyr Ala Trp  Gln Thr Lys Gln Lys  Thr Lys Pro 
    1145                 1150                 1155             


Ile Lys  Asp Ile Tyr Ser Met  Ile Thr Pro Ile Tyr  Lys Lys Leu 
    1160                 1165                 1170             


Cys Ile  Asn Thr Val Val Lys  Ile Asp Gly Phe Lys  Tyr Phe Leu 
    1175                 1180                 1185             


Gly Gly  Arg Thr Glu Asp Tyr  Ile Cys Leu Tyr Pro  Ala Ile Pro 
    1190                 1195                 1200             


Val Val  Leu Asp Glu Gln Ala  Ala Ala Tyr Leu Lys  Ala Val Ser 
    1205                 1210                 1215             


Lys Val  Gly Arg Ala Asp Asp  Val Glu Asn Thr Ala  Gln Ala Ile 
    1220                 1225                 1230             


Asp Leu  Ile Thr Ala Glu Ala  Asn Glu Ala Phe Tyr  Asp Val Leu 
    1235                 1240                 1245             


Leu Lys  Lys Met Glu Ala Lys  Ile Phe Val Lys Ala  Tyr Gln Glu 
    1250                 1255                 1260             


Arg Val  Glu Lys Leu Ala Ser  Glu Thr Ala Arg Glu  Arg Phe Arg 
    1265                 1270                 1275             


Lys Leu  Ser Ile Gly Ala Gln  Cys Ser Cys Leu Leu  Glu Ile Leu 
    1280                 1285                 1290             


Asn Leu  Leu Ala Asn Asn Lys  Thr Thr Ser Asn Leu  Lys Lys Trp 
    1295                 1300                 1305             


Leu Gly  Val Thr Met Ser Arg  Arg Lys Gln Pro Met  Asp Leu Thr 
    1310                 1315                 1320             


Lys Leu  Glu Glu Cys Ile Val  Tyr Glu Thr Ser Ile  Thr Gly Leu 
    1325                 1330                 1335             


Tyr Glu  Lys Ala Val Ser Leu  Leu 
    1340                 1345     


<210>  897
<211>  1346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  897

Met Val Glu His Tyr Ala Ile Gly Phe Asp Ile Gly Thr Gly Ser Thr 
1               5                   10                  15      


Gly Phe Ala Gly Val Asp Pro Asn Gly Lys Leu Val Arg Met Arg Gly 
            20                  25                  30          


Lys Asn Val Tyr Gly Val Tyr Arg Phe Lys Gly Gly Glu Ala Ala Ala 
        35                  40                  45              


Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Ile Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Arg Phe Leu Lys Ser Phe Phe Glu Pro Tyr Ile Ala Pro 
65                  70                  75                  80  


Ile Asp Pro Glu Phe Phe Leu Arg Gln Lys Tyr Ser Ala Asp Ser Pro 
                85                  90                  95      


Leu Asp Pro Lys Gly Ile Arg Ala Asp Lys Gly Leu Leu Ser Asp Met 
            100                 105                 110         


Ser Asp Gln Glu Phe His Ala Lys Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Tyr Gln Leu Met His Glu Lys Arg Gln Phe Asp Val Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Met His His Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu 
145                 150                 155                 160 


Asp Gly Ala Asp Asn Phe Asp Ala Lys Gln Ile Asp Phe Phe Asp Gln 
                165                 170                 175     


Phe Gln Arg Met Ala Ala Val Thr Ala Gly Leu Pro Glu Leu Glu Thr 
            180                 185                 190         


Leu Val Val Pro Asp Glu Thr Gln Ser Asp Ala Phe Lys Arg Val Leu 
        195                 200                 205             


Phe Asp Glu Ser Gln Thr Lys Ser Asp Arg Gln Lys Val Leu Ala Ser 
    210                 215                 220                 


Gln Leu Leu Asp Ile Gln Asp Asn Gln Ser Lys Ala Ile Lys Asp Phe 
225                 230                 235                 240 


Ile Ala Ala Leu Thr Lys Ala Val Val Gly Met Lys Ala Asn Leu Ala 
                245                 250                 255     


Thr Leu Thr Ser Thr Ala Ile Glu Ser Thr Asp Lys Asn Asp Trp Thr 
            260                 265                 270         


Lys Pro Leu Gly Gln Leu Asp Asp Phe Leu Leu Lys His Glu Glu Asp 
        275                 280                 285             


Leu Asp Glu Asp Gly Tyr Ala Leu Ile Asp Ala Ala Asn Gln Met Tyr 
    290                 295                 300                 


Ser Ala Ala Val Leu Ala Ala Leu Val Pro Glu Gly Met Gly Phe Ser 
305                 310                 315                 320 


Glu Ala Met Met Ala Arg Tyr Ala Gln His Lys Lys Gln Leu Ala Leu 
                325                 330                 335     


Tyr Lys Gln Val Ile Ala Ser Leu Pro Asp Lys Thr Gln Arg His Glu 
            340                 345                 350         


Leu Lys Asp Ala Tyr Ala Asp Tyr Ile Glu His Ala Ala Gln Asn Pro 
        355                 360                 365             


Arg Asp Ala Phe Tyr Lys Arg Val Thr Lys Val Leu Glu Gly Val Asp 
    370                 375                 380                 


Ser Ala Asp Ala Arg Thr Val Met Thr Ala Ile Ser His Asp Glu Phe 
385                 390                 395                 400 


Leu Leu Lys Gln Arg Thr Leu Ser Asn Gly Lys Ile Pro Tyr Gln Met 
                405                 410                 415     


Gln Gln His Glu Phe Asp Ala Ile Ile Glu Asn Gln Lys Ala Tyr Tyr 
            420                 425                 430         


Pro Trp Leu Ala Thr Pro Asn Pro Val Glu Ala Asp Ala Lys Arg Leu 
        435                 440                 445             


Pro Tyr Tyr Leu Asp Glu Leu Ile Gly Phe Arg Val Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Met Val Thr Glu Lys Gln Ser Gln Phe Ala Trp Met Ile Arg 
465                 470                 475                 480 


Lys Ala Asp Gly Glu Leu Thr Pro Trp Asn Phe Phe Asp Val Val Asp 
                485                 490                 495     


Arg Glu Lys Thr Ala Asn Arg Phe Ile Gln Arg Met Gln Thr Thr Asp 
            500                 505                 510         


Lys Tyr Ile Leu Gly Glu Asp Val Leu Pro Lys His Ser Ile Leu Tyr 
        515                 520                 525             


Gln Gln Phe Glu Val Leu Asn Glu Leu Asn Asn Ile Arg Val Glu Lys 
    530                 535                 540                 


His Pro Leu Thr Thr Glu Gln Lys Gln Phe Ile Tyr Gln Arg Leu Phe 
545                 550                 555                 560 


Val Asp Gln Gln Arg Lys Thr Ile Thr Lys Lys Asn Leu Gln Asp Thr 
                565                 570                 575     


Leu Val Ala Met Gly Glu Phe Asp Lys Ala Pro Asp Ile Gly Gly Leu 
            580                 585                 590         


Ala Lys Glu Asp Val Met Met Ser Ser Leu Ala Thr Phe Ala Asp Tyr 
        595                 600                 605             


Ser Lys Ile Leu Pro Glu Ala Ile Ser Asp Pro Thr Arg Gln Asn Asp 
    610                 615                 620                 


Ile Glu Asp Ile Ile Ala Trp Ser Thr Ile Phe Glu Asp Thr Gln Ile 
625                 630                 635                 640 


Phe Phe Asp Lys Leu Lys Thr Val Asp Trp Leu Thr Thr Gln Gln Arg 
                645                 650                 655     


Lys Arg Leu Ala Ser Ile Arg Tyr Thr Gly Trp Gly Lys Leu Ser Lys 
            660                 665                 670         


Arg Leu Leu Gln Gln Ile Pro Thr Ser Lys His Thr Asn Val Met Ala 
        675                 680                 685             


Ala Leu Trp Ala Thr Ser Asp Asn Phe Met Gln Ile Leu Gln Asp Glu 
    690                 695                 700                 


Gln Phe Gln Glu Gly Leu Tyr Asp Ala Asn Arg Leu Pro Glu Glu Thr 
705                 710                 715                 720 


Leu Thr Pro Asp Glu Gln Val Glu Lys Ile Leu Asp Glu Ala Tyr Leu 
                725                 730                 735     


Ser Pro Ala Asp Lys Lys Gly Ala Arg Lys Val Ile Lys Val Met Asn 
            740                 745                 750         


Asp Ile Val Arg Ala Asn His Gly Val Leu Pro Glu Gln Val Phe Leu 
        755                 760                 765             


Glu Ser Ala Asp Asp Pro Gln Lys Asn Lys Arg Arg Ser Val Ser Arg 
    770                 775                 780                 


Ser Lys Gln Leu Glu Glu Lys Tyr Arg Gln Ala Ala Gln Ser Phe Ile 
785                 790                 795                 800 


Asp Glu Ala Val Lys Glu Glu Leu Asn Ala Gln Ile Val Asn Ser Ala 
                805                 810                 815     


Glu Phe Asn Asp Arg Leu Val Leu Tyr Phe Thr Gln Asn Gly Arg Asp 
            820                 825                 830         


Met Tyr Thr Gly Lys Arg Leu Asp Ile Glu Asn Leu Gly Ser Tyr Gln 
        835                 840                 845             


Ile Asp His Ile Leu Pro Gln Ser Leu Val Lys Asp Asp Ser Leu Asp 
    850                 855                 860                 


Asn Arg Ala Leu Thr Ala Arg Asp Ile Asn Ala Glu Met Ser Asn Gln 
865                 870                 875                 880 


Phe Val Ser Glu Arg Phe Gly Leu Gly Pro Arg Ala Phe Trp Gln Gln 
                885                 890                 895     


Leu Lys Asn Asn Gly Leu Ile Ser Gly Arg Lys Phe Arg Asn Leu Val 
            900                 905                 910         


Met Gln Arg Asp Glu Ile Asp Lys Tyr Ala Pro Gly Phe Ile Asn Arg 
        915                 920                 925             


Gln Leu Val Glu Thr Arg Gln Ile Ile Lys Thr Val Glu Gln Ile Ile 
    930                 935                 940                 


His Arg Leu Trp Pro Asp Ile Gln Ile Val Thr Ile Lys Ala Asn Leu 
945                 950                 955                 960 


Thr His Gln Val Arg Ser Glu Leu Gly Phe Pro Lys Val Arg Glu Leu 
                965                 970                 975     


Asn Asp Tyr His His Ala Phe Asp Ala Leu Leu Ala Ala Arg Ile Gly 
            980                 985                 990         


Gln Tyr Leu Leu Glu His Tyr Ala  Gly Leu Lys Pro Phe  Phe Thr Tyr 
        995                 1000                 1005             


Gly Glu  Phe Lys Arg Gly Asp  Thr Lys Lys Tyr Gly  Thr Thr Asn 
    1010                 1015                 1020             


Phe Leu  Arg Arg Leu Thr Lys  Asp Asn Lys Asp Lys  Lys Gly Glu 
    1025                 1030                 1035             


Leu Leu  Pro Glu Val Ser Asp  Trp Ile Asp Ser Ile  Asp Ser Ile 
    1040                 1045                 1050             


Tyr Asn  Tyr Lys Lys Ile Pro  Val Val Trp Glu Asn  Thr Thr Asn 
    1055                 1060                 1065             


Met Ser  Ala Leu Phe Asp Gln  Thr Ile Tyr Pro Arg  Pro Asp Arg 
    1070                 1075                 1080             


Asp Thr  Thr Lys Arg Lys Leu  Ile Pro Thr Lys Leu  Asp Lys Pro 
    1085                 1090                 1095             


Thr Asp  Ile Tyr Gly Gly Tyr  Ser Ser Ala Lys Thr  Ala Tyr Met 
    1100                 1105                 1110             


Ala Val  Met Arg Val Gln Thr  Lys Lys Gly Thr Glu  Tyr Pro Val 
    1115                 1120                 1125             


Phe Asn  Ile Pro Val Ser Ile  Ala Ser Gln Leu Asn  Arg Ile Thr 
    1130                 1135                 1140             


Asp Gln  Thr Met Leu Lys Gln  Gln Val Lys Gln Phe  Ile Ile Asn 
    1145                 1150                 1155             


Ala Glu  Gln Ser Ala Ser Lys  Lys Lys Leu Tyr Glu  Ser Gly Asp 
    1160                 1165                 1170             


Val Ile  Leu Ser Arg Val Ala  Leu Gly Gln Arg Phe  Thr Asp Lys 
    1175                 1180                 1185             


Asn Tyr  Gly Glu Val Phe Leu  Ile Ser Asn Met Glu  Val Gln Asn 
    1190                 1195                 1200             


Pro Gln  Ser Leu Trp Leu Gly  Arg Ala Ser Gln Gln  Arg Leu Thr 
    1205                 1210                 1215             


Arg Glu  Pro Glu Gly Asn Glu  Lys Pro Glu Thr Ser  Leu Ala Asp 
    1220                 1225                 1230             


Ile Tyr  Gln Gln Ile Leu Lys  Gln Val Asp Arg Tyr  Phe Leu Ala 
    1235                 1240                 1245             


Phe Asn  Ile Arg Gln Gln Arg  Gln Lys Leu Leu Gly  Thr Leu Asp 
    1250                 1255                 1260             


Gln Phe  Thr Ser Leu Pro Val  Gly Arg Val Asn Glu  Gln Thr Arg 
    1265                 1270                 1275             


Thr Asp  Gln Asn Gln Arg Ser  Ile Ile Ala Arg Val  Met Ile Val 
    1280                 1285                 1290             


Leu Gly  Lys Gly Ser Arg Glu  Glu Lys Phe Pro Glu  Phe Gly Leu 
    1295                 1300                 1305             


Ser Ala  Leu Gly Arg Leu Val  Arg Thr Gly Gly Ile  Thr Leu Ser 
    1310                 1315                 1320             


Glu Asp  Ala Gln Ile Ile Tyr  Gln Ser Pro Thr Gly  Leu Phe Glu 
    1325                 1330                 1335             


Arg Arg  Val Ala Leu Lys Asp  Leu 
    1340                 1345     


<210>  898
<211>  1346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  898

Met Ala Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asp Ile Glu Asn Asn Asn Glu Phe Thr Leu Ile Glu Lys 
            20                  25                  30          


Gly Val Arg Ile Phe Gln Glu Gly Val Lys Ile Glu Lys Gly Ile Glu 
        35                  40                  45              


Gly Ser Lys Ala Ala Glu Arg Thr Thr Tyr Arg Ser Ala Arg Arg Ile 
    50                  55                  60                  


Lys Tyr Arg Arg Lys Leu Arg Lys Ile Gln Leu Leu Gly Val Leu Ser 
65                  70                  75                  80  


Glu Asn Gly Tyr Cys Pro Phe Leu Ser Lys Asp Glu Leu Asp Ser Trp 
                85                  90                  95      


Arg Tyr Lys Lys Val Tyr Pro Thr Asn Lys Ser Phe Leu Asn Trp Gln 
            100                 105                 110         


Lys Thr Asn Glu Ile Gln Leu Glu Asn Pro Tyr Phe Tyr Arg Ala Leu 
        115                 120                 125             


Leu Val Glu Lys Lys Leu Asn Leu Asn Ile Glu Glu Asp Arg Phe Lys 
    130                 135                 140                 


Ile Gly Arg Ala Phe Tyr His Leu Ala Gln Arg Arg Gly Phe Leu Ser 
145                 150                 155                 160 


Asn Arg Met Glu Gly Thr Lys Glu Asn Asp Gly Thr Val Ile Lys Ser 
                165                 170                 175     


Ile Thr Glu Ile Ser Thr Ala Lys Gly Asp Lys Thr Leu Gly Gln Tyr 
            180                 185                 190         


Phe Tyr Glu Arg Tyr Lys Lys Gly Glu Lys Ile Arg Asp Thr Tyr Thr 
        195                 200                 205             


His Arg Glu Glu His Tyr Leu Glu Glu Phe Val Arg Ile Cys Glu Phe 
    210                 215                 220                 


Gln Gln Leu Asp Asn Lys Phe Ala Glu Lys Val Arg Lys Ala Ile Phe 
225                 230                 235                 240 


Tyr Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Ile Gly Lys Cys Val 
                245                 250                 255     


Phe Glu Pro Lys Lys Ser Arg Cys Ser Val Ser Arg Pro Glu Phe Glu 
            260                 265                 270         


Glu Tyr Arg Met Leu Cys Phe Leu Asn Asn Ile Lys Ile Lys Thr Pro 
        275                 280                 285             


Ser Asp Glu Asn Leu Arg Phe Leu Thr Asp Asn Glu Lys Glu Lys Leu 
    290                 295                 300                 


Lys Pro Gln Phe Phe Arg Lys Ser Lys Asp His Phe Asp Phe Glu Asp 
305                 310                 315                 320 


Leu Val Lys Gln Leu Ala Pro Lys Asn Gln Phe Lys Phe Tyr Lys Ser 
                325                 330                 335     


Arg Asp Ile Leu Pro Gly Asn Trp Leu Phe Asn Tyr Ser Met Asn Thr 
            340                 345                 350         


Thr Val Ser Gly Cys Pro Ile Ser Ala Arg Leu Lys Asp Leu Phe Gly 
        355                 360                 365             


Glu Asn Phe Met Asp Ile Lys Phe Asp Tyr Ile Arg Glu Lys Asp Gly 
    370                 375                 380                 


Lys Lys Ser Val Ile Asp Ile His Asp Ile Trp His Val Leu Phe Thr 
385                 390                 395                 400 


Tyr Asp Ser Glu Glu Lys Leu Lys Glu Phe Ala Arg Asn Arg Leu Asn 
                405                 410                 415     


Leu Asp Asp Ile Lys Ile Lys Glu Phe Gln Arg Ile Gln Leu Lys Lys 
            420                 425                 430         


Glu Tyr Ala Ala Leu Ser Leu Lys Ala Ile Arg Lys Ile Leu Pro Phe 
        435                 440                 445             


Leu Arg Glu Gly Leu Ile Tyr Ser His Ala Val Phe Ile Ala Asn Met 
    450                 455                 460                 


Glu Thr Ala Ile Pro Ser Glu Ile Trp Asn Asn Tyr Thr Asn Arg Asn 
465                 470                 475                 480 


Ile Ile Lys Asn Glu Ile Tyr Asn Ile Ile Ser Asn Gln Thr Glu Asp 
                485                 490                 495     


Lys Gln Val Val Asp Ile Val Asn Gly Ile Ile Lys Ser Ser Arg Ile 
            500                 505                 510         


Glu Asn Ser Val Trp Ser Asn Glu Ala Val Glu Phe Phe Lys Lys Asp 
        515                 520                 525             


Leu Asn Ser Lys Ile Lys Asn Tyr Tyr Gly Ile Lys Thr Tyr Asn Asp 
    530                 535                 540                 


Phe Pro Glu Glu Lys Lys Ile Arg Ile Glu Thr Tyr Ala Phe Asn Leu 
545                 550                 555                 560 


Phe Lys Gln Gln Met Met Lys Asn Phe Gly Arg Gly Glu Phe Ala Ala 
                565                 570                 575     


Val Gln Arg Ile Asp Glu Arg Leu Lys Leu Phe Leu Thr Asp Asn Phe 
            580                 585                 590         


Asn Ile Asp Pro Ile Leu Leu Lys Lys Ile Tyr His Pro Ser Ala Ile 
        595                 600                 605             


Glu Val Tyr Gln Pro Pro Val Arg Lys Asn Asp Gly Lys Leu Tyr Leu 
    610                 615                 620                 


Gly Ser Pro Met Val Ser Ser Ile Arg Asn Pro Met Ala Met Arg Ala 
625                 630                 635                 640 


Leu His Gln Leu Arg Lys Leu Ile Asn Glu Leu Ile Arg Lys Glu Leu 
                645                 650                 655     


Ile Asp Ser Asn Thr Lys Val Asn Ile Glu Met Ala Arg Glu Leu Met 
            660                 665                 670         


Asn Ala Asn Glu Arg Lys Ala Tyr Gln Asn Trp Gln Asn Asp Arg Glu 
        675                 680                 685             


Lys Ile Arg Lys Glu Tyr Ala Leu Lys Ile Lys Gln His Leu Asp Val 
    690                 695                 700                 


Asp Lys Glu Pro Ser Asp Val Asp Ile Leu Lys Tyr Gln Leu Trp Glu 
705                 710                 715                 720 


Glu Gln Asn His Lys Cys Ile Tyr Thr Gly Lys Glu Ile Asn Ile Ser 
                725                 730                 735     


Asp Phe Leu Gly Ser Asn Pro Lys Phe Asp Ile Glu His Thr Ile Pro 
            740                 745                 750         


Arg Ser Ile Ser Phe Asp Asn Ser Gln Ser Asn Lys Thr Leu Cys Glu 
        755                 760                 765             


Asn Tyr Phe Asn Arg Ser Val Lys Lys Asn Arg Ile Pro Ala Ala Leu 
    770                 775                 780                 


Pro Asn His Gly Glu Ile Ile Glu Arg Leu Glu Asn Trp Lys Leu Lys 
785                 790                 795                 800 


Tyr Glu Glu Leu Glu Lys Gln Ile Gln Tyr Ala Val Arg Gln Ala Lys 
                805                 810                 815     


Gly Ala Gly Asp Lys Asp Ser Lys Asp Arg Ala Ile Gln Lys Arg His 
            820                 825                 830         


Lys Leu Lys Phe Glu Tyr Asp Tyr Trp Lys Asn Lys Tyr Asp Arg Phe 
        835                 840                 845             


Val Met Glu Asp Val Pro Glu Gly Phe Lys Asn Ser Gln Leu Val Asp 
    850                 855                 860                 


Thr Gly Ile Ile Thr Lys Tyr Ser Arg Met Tyr Leu Asn Thr Leu Phe 
865                 870                 875                 880 


Asp Lys Val Tyr Thr Val Lys Gly Asn Thr Val Ala Asp Phe Arg Lys 
                885                 890                 895     


Leu Trp Gly Ile Gln Asp Glu Tyr Glu Lys Lys Ala Arg Val Asn His 
            900                 905                 910         


Ser His His Cys Val Asp Ala Ile Thr Ile Ala Cys Ile Thr Lys Ser 
        915                 920                 925             


Asn Tyr Glu Asn Leu Ala Lys Phe Tyr His Asp Trp Glu Glu Leu His 
    930                 935                 940                 


Val Lys Gly Ile Asp Gln Leu Pro Pro Met Asn Lys Pro Trp Lys Thr 
945                 950                 955                 960 


Phe Thr Glu Asp Ile Lys Ala Ile Gln Tyr Glu Ile Leu Val Ser His 
                965                 970                 975     


Tyr Thr Pro Asp Asn Leu Pro Lys Gln Ser Lys Lys Ile Leu Arg Lys 
            980                 985                 990         


Lys Gly Arg Ile Gln Lys Asn Glu  Asn Gly Asn Pro Ile  Tyr Leu Lys 
        995                 1000                 1005             


Gly Asp  Thr Val Arg Gly Ser  Leu His Gln Gln Thr  Phe Tyr Gly 
    1010                 1015                 1020             


Ala Ile  Glu Lys Thr Glu Val  Asn Lys Lys Gly Glu  Pro Glu Lys 
    1025                 1030                 1035             


Val Ile  Lys Tyr Val Val Arg  Lys Ser Leu Asp Val  Leu Asp Asp 
    1040                 1045                 1050             


Ser Ser  Val Lys Asn Ile Val  Asp Asp Ile Val Lys  Glu Ile Val 
    1055                 1060                 1065             


Gln Asn  Ala Arg Ile Lys Glu  Lys Asp Phe Ile Lys  Glu Leu Glu 
    1070                 1075                 1080             


Ile Gln  Lys Arg Lys Leu Ala  Lys Ala Glu Glu Asn  Lys Val Val 
    1085                 1090                 1095             


Gln Ile  Gln Ala Glu Ile Asp  Lys Leu Asn Lys Gln  Val Ala Asp 
    1100                 1105                 1110             


Leu Tyr  Thr Met Pro Asn Lys  Asn Gly Asn Pro Ile  Pro Ile Lys 
    1115                 1120                 1125             


Lys Val  Arg Ile Phe Gln Thr  Ser Val Thr Asn Pro  Ile His Leu 
    1130                 1135                 1140             


Lys Gln  Gln Arg Asp Lys Ser  Gln Lys Thr Ser Lys  Pro His Lys 
    1145                 1150                 1155             


Glu Phe  Tyr Tyr Ala Ala Asn  Asp Ser Asn Tyr Met  Met Ala Ile 
    1160                 1165                 1170             


Tyr Glu  Gly Lys Asp Glu Lys  Gly Lys Val Lys Arg  Asp Phe Lys 
    1175                 1180                 1185             


Leu Leu  Asn Asn Leu Glu Ala  Gly Glu Phe Phe Lys  Tyr Ser Val 
    1190                 1195                 1200             


His Lys  Asp Leu Lys Glu Lys  Asn Leu Glu Asn Ile  Arg Asn Val 
    1205                 1210                 1215             


Ile Pro  Asp Lys Val Ser Ala  Gly Lys Ile Glu Ile  Pro Leu Lys 
    1220                 1225                 1230             


Ala Ile  Ile Lys Thr Gly Thr  Met Val Ile Leu Trp  Glu Asn Ser 
    1235                 1240                 1245             


Pro Asp  Glu Val Trp Asp Leu  Thr Ile Ser Glu Val  Asp Lys Arg 
    1250                 1255                 1260             


Leu Tyr  Lys Val Val Gly Met  Ser Val Asn Arg Ile  Lys Ser Asp 
    1265                 1270                 1275             


Val Lys  Thr Phe Glu Phe Gly  Asn Ile Val Leu Arg  His Asn Lys 
    1280                 1285                 1290             


Glu Ala  Ser Pro Ala Thr Leu  Leu Lys Ile Gln Asp  Gly Glu Phe 
    1295                 1300                 1305             


Lys Ser  Asp Glu Asn Tyr Ile  Ala Gln Arg Lys Leu  Ser His Asn 
    1310                 1315                 1320             


Gln Phe  Asn Ala Leu Val Glu  Gly Val Asp Phe Ser  Leu Ser Val 
    1325                 1330                 1335             


Leu Gly  Glu Ile Lys Arg Leu  Lys 
    1340                 1345     


<210>  899
<211>  1347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  899

Met Gly Glu Asp Gly Lys Pro Val Arg Val Lys Gly Lys Thr Ala Ile 
1               5                   10                  15      


Gly Ala Arg Leu Phe Gln Glu Gly His Val Ala Ala Asp Arg Arg Met 
            20                  25                  30          


Phe Arg Thr Thr Arg Arg Arg Leu Asn Arg Arg Lys Trp Arg Leu Lys 
        35                  40                  45              


Leu Leu Asp Glu Ile Phe Asp Pro Tyr Ile Thr Pro Val Asp Ser Thr 
    50                  55                  60                  


Phe Phe Ala Arg Leu Lys Gln Ser Asn Leu Ser Pro Lys Asp Ser Arg 
65                  70                  75                  80  


Lys Glu Phe Lys Gly Ser Met Leu Phe Pro Asp Leu Thr Asp Lys Gln 
                85                  90                  95      


Tyr His Glu Asp Tyr Pro Thr Ile Tyr His Leu Arg Arg Ala Leu Met 
            100                 105                 110         


Thr Glu Asp Lys Lys Phe Asp Ile Arg Met Val Tyr Leu Ala Ile His 
        115                 120                 125             


His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn Ser Ala Thr Val Asp 
    130                 135                 140                 


Thr Phe Lys Ala Ser Lys Val Asp Phe Lys Lys Ser Phe Asn Gln Leu 
145                 150                 155                 160 


Asn Glu Leu Tyr Ser Ala Ile Asn Pro Glu Asp Ala Phe Gln Val Asp 
                165                 170                 175     


Met Asn Lys Ser Asp Glu Thr Gly Arg Leu Phe Leu Asp Ser Ser Ile 
            180                 185                 190         


Arg Lys Phe Asp Lys Gln Lys Gln Ile Ser Lys Leu Ile Pro Val Val 
        195                 200                 205             


Val Glu Asp Lys Glu Ala Lys Lys Leu Asn Gly Lys Ile Ala Leu Glu 
    210                 215                 220                 


Ile Thr Lys Ala Ile Leu Gly Tyr Lys Ser Lys Leu Asn Val Val Thr 
225                 230                 235                 240 


Gln Thr Glu Pro Glu Asp Pro Lys Lys Trp Ala Val Lys Phe Asp Asp 
                245                 250                 255     


Glu Asp Ile Asp Ser Lys Leu Asn Asp Ile Leu Pro Glu Met Asp Glu 
            260                 265                 270         


Asn Gln Gln Ser Ile Val Glu Ile Ile Gln Asp Leu Tyr Ser Gln Val 
        275                 280                 285             


Thr Leu Asn Gln Ile Val Pro Asn Gly Met Ser Leu Ser Asp Ser Met 
    290                 295                 300                 


Ile Lys Lys Tyr Asn Asp His His Glu His Leu Lys Leu Tyr Lys Lys 
305                 310                 315                 320 


Leu Ile Asp Asn Ile Ser Asp Thr Lys Lys Arg Ser Ala Leu Lys Thr 
                325                 330                 335     


Ala Tyr Asp Lys Tyr Val Gly Asp Asp Gly Lys Thr Ile Glu Gln Asp 
            340                 345                 350         


Glu Phe Tyr Ser Asn Val Lys Lys Asn Leu Asp Asp Ser Asp Leu Ser 
        355                 360                 365             


Lys Gln Ile Ala Asp Leu Ile Glu Ala Glu Lys Phe Met Pro Lys Gln 
    370                 375                 380                 


Arg Thr Ser Gln Asn Gly Val Ile Pro His Gln Leu His Gln Arg Glu 
385                 390                 395                 400 


Leu Asp Glu Ile Ile Glu His Gln Ser Lys Tyr Tyr Pro Trp Phe Ala 
                405                 410                 415     


Glu Ala Asn Pro Asn Lys His Asp Leu His Leu Ala Lys Tyr Lys Ile 
            420                 425                 430         


Glu Glu Leu Val Ala Phe Arg Ile Pro Tyr Tyr Val Gly Pro Met Ile 
        435                 440                 445             


Thr Pro Glu Asp Gln Glu Lys Ser Ser Gln Thr Ile Phe Ala Trp Met 
    450                 455                 460                 


Lys Arg Lys Glu Pro Gly Gln Ile Thr Pro Trp Asn Phe Asp Glu Lys 
465                 470                 475                 480 


Val Asp Arg Met Glu Ser Ala Asn Arg Phe Ile Lys Arg Met Thr Thr 
                485                 490                 495     


Lys Asp Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Asp Gln Ser Leu 
            500                 505                 510         


Leu Tyr Glu Lys Phe Lys Val Leu Asn Glu Leu Asn Met Val Arg Val 
        515                 520                 525             


Asn Asp His Pro Leu Lys Val Ser Glu Lys Gln Ala Ile Tyr Arg Asp 
    530                 535                 540                 


Leu Phe Glu Asn Gln Lys Arg Ile Ser Val Lys Asn Leu Gln Asn Tyr 
545                 550                 555                 560 


Val Lys Ser Lys Ser Gly Leu Pro Thr Gly Pro Lys Ile Ser Gly Leu 
                565                 570                 575     


Ser Asp Pro Asp His Phe Asn Ser Ser Leu Gly Thr Tyr Asn Asp Phe 
            580                 585                 590         


Lys Lys Met Phe Gly Asp Lys Val Asp Asp Pro Asp Leu Gln Asp Asp 
        595                 600                 605             


Phe Glu Lys Met Ile Glu Trp Ser Thr Ile Phe Glu Asp Lys Lys Ile 
    610                 615                 620                 


Leu Arg Ala Lys Leu Asp Glu Val Ala Trp Leu Ser Asp Thr Gln Lys 
625                 630                 635                 640 


Asn Glu Phe Lys Glu Ile Arg Tyr Gln Gly Trp Gly Arg Leu Ser Lys 
                645                 650                 655     


Lys Leu Leu Thr Gly Ile Val Asp Gly Asn Gly Gln Arg Ile Ile Asp 
            660                 665                 670         


Glu Leu Trp Asn Thr Asn Lys Asn Phe Met Gln Ile Gln Ser Asp Asp 
        675                 680                 685             


Ser Phe Ala Lys Arg Ile His Glu Ala Asn Ala Asn Gln Met Lys Ala 
    690                 695                 700                 


Thr Asp Val Glu Asp Val Leu Ala Asp Ala Tyr Thr Ser Pro Gln Asn 
705                 710                 715                 720 


Lys Lys Ala Ile Arg Gln Val Val Lys Val Val Asp Asp Ile Gln Lys 
                725                 730                 735     


Ala Met Gly Gly Val Ala Pro Lys Phe Ile Ser Ile Glu Phe Thr Arg 
            740                 745                 750         


Ser Glu Asp Arg Asn Pro Arg Arg Thr Ile Ser Arg Gln Arg Gln Leu 
        755                 760                 765             


Glu Asn Thr Leu Lys Asp Thr Ala Gln Ala Leu Val Lys Ser Ile Asn 
    770                 775                 780                 


Pro Asp Ile Leu Ser Glu Leu Asp Asn Ala Ser Lys Ser Lys Lys Gly 
785                 790                 795                 800 


Leu Thr Asp Arg Leu Tyr Leu Tyr Phe Thr Gln Leu Gly Lys Asp Met 
                805                 810                 815     


Tyr Thr Gly Lys Pro Ile Asn Ile Asp Glu Ile Ser Asn Tyr Asp Ile 
            820                 825                 830         


Asp His Ile Phe Pro Gln Ala Phe Ile Lys Asp Asp Ser Leu Asn Asn 
        835                 840                 845             


Arg Val Leu Val Ser Lys Ala Gly Asn Asn Gly Lys Ser Ala Asn Val 
    850                 855                 860                 


Pro Leu Lys Leu Phe Gly Ala Lys Met Gly Gly Tyr Trp Lys Gln Leu 
865                 870                 875                 880 


Ala Asp Ala Gly Leu Ile Gly Gln Arg Lys Leu Lys Asn Leu Gln Thr 
                885                 890                 895     


Asn Pro Asp Thr Ile Ser Lys Phe Ala Met His Gly Phe Ile Arg Arg 
            900                 905                 910         


Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Val Ala Asn Ile Leu 
        915                 920                 925             


Gly Asp Lys Tyr Arg Asn Asp Asp Thr Lys Ile Ile Glu Ile Thr Ala 
    930                 935                 940                 


Arg Met Asn His Gln Met Arg Asp Glu Phe Gly Phe Ile Lys Asn Arg 
945                 950                 955                 960 


Glu Ile Asn Asp Tyr His His Ala Phe Asp Ala Tyr Leu Thr Ala Phe 
                965                 970                 975     


Leu Gly His Tyr Leu Tyr His Arg Tyr Ile Lys Leu Arg Pro Tyr Phe 
            980                 985                 990         


Val Tyr Gly Asp Phe Lys Lys Phe  Arg Glu Asp Lys Val  Thr Met Arg 
        995                 1000                 1005             


Asn Phe  Asn Phe Leu His Asp  Leu Thr Asp Asp Lys  Gln Glu Arg 
    1010                 1015                 1020             


Ile Ala  Asp Thr Glu Thr Gly  Glu Val Ile Trp Asp  Arg Lys Arg 
    1025                 1030                 1035             


Ser Ile  Gln Gln Leu Arg Asp  Val Tyr His Tyr Lys  Phe Met Leu 
    1040                 1045                 1050             


Ile Ser  Gln Glu Val Phe Ser  Leu Asn Lys Glu Met  Phe Lys Gln 
    1055                 1060                 1065             


Lys Leu  Phe Pro Ala Thr Leu  Ala Lys Lys Ser Lys  Leu Ile Gln 
    1070                 1075                 1080             


Ile Lys  Lys Asp Lys Pro Val  Asp Ile Tyr Gly Gly  Tyr Ser Asn 
    1085                 1090                 1095             


Asn Thr  Asp Ala Tyr Leu Thr  Val Val Lys Leu Lys  Gly Ser Lys 
    1100                 1105                 1110             


Glu Asp  Lys Tyr Lys Ile Val  Gly Val Pro Met Arg  Ala Leu Asp 
    1115                 1120                 1125             


Asn Leu  Lys His Ala Glu Lys  Glu Ser Pro Gln Ser  Tyr Thr Ala 
    1130                 1135                 1140             


Glu Leu  Arg Lys Ala Leu Glu  Pro Gln Phe Thr Lys  Ile Lys Lys 
    1145                 1150                 1155             


Asn Arg  Lys Thr Gly Glu Val  Ser Lys Val Val Thr  Asn Phe Asp 
    1160                 1165                 1170             


Ile Val  Leu Gly Arg Leu Phe  Tyr Arg Gln Met Val  Ile Asp Gly 
    1175                 1180                 1185             


Ser Glu  Lys Ser Leu Ile Ala  Ser Ser Val Tyr Gln  Tyr Asn Ala 
    1190                 1195                 1200             


Lys Gln  Leu Val Leu Ser Asp  Gln Ala Ile Arg Thr  Ile Ser Asp 
    1205                 1210                 1215             


Gln Glu  Gly Ile Asp Glu Phe  Glu Leu Ser Ser Gln  Tyr Asp Lys 
    1220                 1225                 1230             


Val Tyr  Asp Glu Ile Val Gln  Lys Val Glu Lys Leu  Leu Pro Ile 
    1235                 1240                 1245             


Phe Gln  His Arg His Phe Gln  Glu Arg Leu Asn Asn  Gly Lys Ser 
    1250                 1255                 1260             


Leu Phe  Val Asn Leu Pro Val  His Asp Val Tyr Glu  Gly Asn Lys 
    1265                 1270                 1275             


Leu Glu  Gln Val Gly Lys Lys  His Ile Ile Asn Ser  Ile Leu Gly 
    1280                 1285                 1290             


Gly Ile  His Ala Asn Ala Asp  Arg Pro Asp Ile Asp  Leu Phe Gly 
    1295                 1300                 1305             


Ile Lys  Asn Leu Gly Gln Phe  Ser Ser Thr Ser Gly  Ile Asn Leu 
    1310                 1315                 1320             


Ser Ser  Asp Ala Ile Ile Cys  Tyr Gln Ser Pro Thr  Gly Leu Phe 
    1325                 1330                 1335             


Glu Arg  Arg Val Val Leu Lys  Asp Leu 
    1340                 1345         


<210>  900
<211>  1347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  900

Met Thr Lys Arg Glu Glu Pro Tyr Asn Val Gly Leu Asp Ile Gly Ser 
1               5                   10                  15      


Ser Ser Val Gly Trp Ala Val Val Asp Asn Asn Tyr His Leu Leu Asn 
            20                  25                  30          


Ile Lys Lys Asn Asn Leu Trp Gly Ala Arg Leu Phe Lys Glu Ala Glu 
        35                  40                  45              


Thr Ala Gln Val Thr Arg Gly His Arg Ser Met Arg Arg Arg Tyr Arg 
    50                  55                  60                  


Arg Arg Arg Asn Arg Leu Asn Trp Leu Asp Glu Leu Phe Ala Asp Glu 
65                  70                  75                  80  


Leu Ala Lys Ile Asp Pro Ser Phe Leu Leu Arg Met Lys Asn Ser Trp 
                85                  90                  95      


Val Ser Lys Lys Glu Ser Thr Arg Lys Arg Asp Pro Tyr Asn Leu Phe 
            100                 105                 110         


Ile Asp Glu Lys Tyr Asn Asp Val Asp Tyr Phe Asn Gln Tyr Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Lys Glu Leu Ile Thr Glu Asp Lys Lys Val Asp 
    130                 135                 140                 


Ile Arg Leu Ile Tyr Leu Ala Ile His Asn Ile Ile Lys Tyr Arg Gly 
145                 150                 155                 160 


Asn Phe Thr Leu Glu Asn Gln Asn Phe Asp Ile Ser Gln Leu Ser Ser 
                165                 170                 175     


Asn Phe Ser Gln Gln Ile Ser Asp Phe Phe Ala Leu Phe Ser Asp Phe 
            180                 185                 190         


Gly Val Ile Met Pro Glu Asp Phe Asp Pro Asp Lys Ile Ser Asp Ile 
        195                 200                 205             


Leu Leu Asn Pro Asn Leu Ser Pro Ser Gly Lys Val Ser Glu Ala Ile 
    210                 215                 220                 


Ala Thr Ile Ser Pro Lys Thr Asn Val Lys Ala Lys Ile Lys Ile Ile 
225                 230                 235                 240 


Leu Leu Leu Leu Val Gly Asn Asn Gly Asp Leu Lys Lys Leu Phe Asp 
                245                 250                 255     


Leu Glu Thr Thr Glu Lys Ile Ala Val Lys Leu Ser Ser Arg His Ile 
            260                 265                 270         


Asp Ser Glu Leu Pro Ile Ile Leu Ser Glu Leu Asn Glu Gln Gln Glu 
        275                 280                 285             


Asn Ile Ile Thr Ile Ala Asn Ser Ile Phe Gly Ser Ile Ile Leu Lys 
    290                 295                 300                 


Asp Phe Leu Gly Asp Glu Thr Ser Ile Ser Ala Ala Lys Val Ile Ser 
305                 310                 315                 320 


Phe Glu Asp His Lys Gln Asp Leu Gln Lys Leu Lys Thr Met Trp Arg 
                325                 330                 335     


Glu Thr Ser Asn Lys Glu Ala Val Lys Ala Gly Lys Lys Ala Tyr Glu 
            340                 345                 350         


Asp Tyr Ile Gly His Glu Asp Ser Glu Thr Phe Tyr Lys Lys Ile Lys 
        355                 360                 365             


Lys Phe Leu Glu Lys Ala Gln Pro Val Asp Leu Ala Asn Lys Ala Leu 
    370                 375                 380                 


Ala Glu Ile Glu Leu Glu Asn Tyr Leu Pro Lys Gln Arg Asn Arg Asn 
385                 390                 395                 400 


Asn Thr Val Ile Pro Tyr Gln Leu Asn Glu Asn Glu Leu Ile Ala Ile 
                405                 410                 415     


Leu Asp His Gln Glu Lys Tyr Tyr Pro Phe Leu Lys Glu Asn Arg Asp 
            420                 425                 430         


Lys Ile Leu Ser Leu Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
        435                 440                 445             


Leu Gln Asp Ser Asn Asn Asn Arg Phe Ser Trp Met Thr Arg Lys Ala 
    450                 455                 460                 


Ser Gly Ala Ile Arg Pro Trp Asn Phe Ser Glu Lys Val Asn Val Glu 
465                 470                 475                 480 


Gln Ser Ser Asn Asp Phe Ile Lys Arg Met Arg Ser Thr Asp Thr Tyr 
                485                 490                 495     


Leu Ile Gly Glu Pro Val Leu Pro Lys Lys Ser Leu Ile Tyr Gln Cys 
            500                 505                 510         


Tyr Glu Val Leu Ser Glu Leu Asn Asn Thr Arg Val Lys Asp Gly Ser 
        515                 520                 525             


Ser Asn Pro Lys Arg Leu Asp Val Thr Ile Lys Gln Arg Ile Tyr Asn 
    530                 535                 540                 


Glu Ile Phe Lys Asn Gln Lys Ser Val Ser Val Lys Val Leu Gln Asn 
545                 550                 555                 560 


Trp Leu Ile Lys Glu Ser Tyr Phe Lys Ser Pro Glu Ile Ser Gly Leu 
                565                 570                 575     


Ala Asp Lys Lys Lys Tyr Leu Ser Ser Leu Ser Thr Tyr Ile Asp Phe 
            580                 585                 590         


Lys Lys Ile Phe Gly Gln Arg Phe Val Asp Asp Pro Val Asn Ser Pro 
        595                 600                 605             


Gln Leu Glu Glu Leu Ala Glu Trp Leu Thr Leu Phe Glu Asp Lys Lys 
    610                 615                 620                 


Ile Leu Leu Ile Lys Leu Gln Asn Ser Lys Tyr Ser Tyr Asp Gln Ala 
625                 630                 635                 640 


Thr Ile Asn Lys Leu Ser Thr Met Arg Tyr Gln Gly Thr Gly Lys Leu 
                645                 650                 655     


Ser Lys Lys Leu Leu Val Asp Leu Lys Thr Thr Lys Lys Ser Ile Gly 
            660                 665                 670         


Lys Ser Gly Ala Glu Ser Leu Ser Ile Leu Asp Leu Met Trp Ser Thr 
        675                 680                 685             


Lys Asp Asn Phe Met Gln Ile Ile His Asp Ala Asp Tyr Thr Phe Glu 
    690                 695                 700                 


Gln Gln Ile Lys Glu Phe Asn Tyr Asp Thr Glu Asp Glu Leu Thr Pro 
705                 710                 715                 720 


Leu Glu Lys Val Ala Asn Leu His Gly Ser Pro Ala Leu Lys Arg Gly 
                725                 730                 735     


Leu Asn Gln Ser Ile Lys Val Val Ala Asp Ile Val Lys Phe Met Gly 
            740                 745                 750         


His Asp Pro Glu Lys Ile Phe Ile Glu Phe Thr Arg Ser Asp Asp Phe 
        755                 760                 765             


Ser Lys Leu Thr Ile Ser Arg Tyr Arg Arg Ile Lys Lys Gln Tyr Leu 
    770                 775                 780                 


Glu Ile Ala Lys Ala Ile Lys Lys Ile Pro Ala Glu Phe Lys Asp Ile 
785                 790                 795                 800 


Lys Glu Tyr Gln Thr Gln Leu Glu Glu Asn Lys Gly Lys Leu Ala Ser 
                805                 810                 815     


Glu Arg Leu Met Leu Tyr Phe Leu Gln Cys Gly His Ser Leu Tyr Ser 
            820                 825                 830         


Asn Lys Pro Ile Asp Leu Asn Met Ile Asn Ser Ser Lys Tyr His Val 
        835                 840                 845             


Asp His Ile Leu Pro Gln Ser Tyr Ile Lys Asp Asp Ser Leu Glu Asn 
    850                 855                 860                 


Lys Ala Leu Val Leu Ala Ser Glu Asn Glu Asn Lys Ile Asp Asn Met 
865                 870                 875                 880 


Leu Ile Ser His Asp Ile Ile Ala Thr Asn Leu Pro Arg Trp Gln Ala 
                885                 890                 895     


Leu Lys Asp Gln Asn Leu Met Gly Ser Lys Lys Phe Ala Asp Leu Thr 
            900                 905                 910         


Arg Thr Thr Val Thr Glu Asn Gln Lys Lys Gly Phe Ile Gln Arg Gln 
        915                 920                 925             


Leu Val Gln Thr Ser Gln Ile Val Lys Asn Ile Thr Leu Ile Leu Asn 
    930                 935                 940                 


Asp Leu Tyr Lys Asn Thr Ser Cys Ile Glu Thr Arg Ala Thr Leu Ser 
945                 950                 955                 960 


Ser Glu Phe Arg Lys Ala Phe Ser Asn Phe Asp Glu Thr Thr Tyr His 
                965                 970                 975     


Tyr Gln Phe Pro Glu Phe Val Lys Asn Arg Asp Val Asn Asp Phe His 
            980                 985                 990         


His Ala Gln Asp Ala Phe Leu Ala  Cys Val Ile Gly Glu  Tyr Gln Leu 
        995                 1000                 1005             


Lys Lys  Tyr Pro Lys Asp Asn  Leu Arg Leu Val Tyr  Asp Gln Tyr 
    1010                 1015                 1020             


Ser Lys  Phe Leu Asp Ser Leu  Lys Lys Asp Thr Arg  Lys Lys Asn 
    1025                 1030                 1035             


Gly Arg  Met Pro Arg Tyr Thr  Gln Asn Gly Phe Ile  Ile Gly Ser 
    1040                 1045                 1050             


Met Phe  Asn Gly Lys Thr Tyr  Val Asp Asp Asn Gly  Glu Ile Ile 
    1055                 1060                 1065             


Trp Asp  Gln Lys Ile Lys Glu  Ser Ile Arg Lys Thr  Phe Asn Tyr 
    1070                 1075                 1080             


His Gln  Phe Asn Val Val Arg  Gln Thr Ile Glu Gln  His Gly Lys 
    1085                 1090                 1095             


Leu Phe  Asn Asp Thr Ile Gln  Pro His Ser Asp Arg  Tyr Lys Leu 
    1100                 1105                 1110             


Ile Pro  Leu Lys Thr Asn Arg  Asp Pro Ala Ile Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Asn Asn  Asp Asn Asn Ala Tyr  Ser Val Val Leu Asp  Val Asp Gly 
    1130                 1135                 1140             


Lys Lys  Lys Ile Asn Gly Ile  Pro Ile Arg Ile Ala  Asn Gln Leu 
    1145                 1150                 1155             


Lys Ser  Asp Glu Leu Asp Leu  Ser Ser Trp Leu Glu  Asn Asn Ile 
    1160                 1165                 1170             


Lys His  Lys Lys Pro Met Thr  Ile Leu Ile Asp Lys  Val Pro Lys 
    1175                 1180                 1185             


Tyr Gln  Arg Ile Ile Asn Glu  Glu Thr Gly Asp Leu  Leu Ile Thr 
    1190                 1195                 1200             


Ser Ala  Asn Glu Val Ile Asn  Asn Val Gln Leu Phe  Leu Pro Ser 
    1205                 1210                 1215             


Met Tyr  Thr Ala Leu Ile Ser  Leu Leu Asp Ser Thr  Lys Thr Glu 
    1220                 1225                 1230             


Met Tyr  Ser Lys Leu Leu Ser  Asn Tyr Glu Ala Asn  Ile Leu Ile 
    1235                 1240                 1245             


Asp Ile  Tyr Asp Tyr Leu Leu  Thr Lys Leu Lys Asn  Asn Tyr Pro 
    1250                 1255                 1260             


Leu Tyr  Arg Lys Glu Trp Ala  Lys Leu Ala Glu His  Arg Asp Asp 
    1265                 1270                 1275             


Phe Ile  Glu Ser Asp Leu Val  Thr Gln Ala Ser Thr  Leu Gln Gln 
    1280                 1285                 1290             


Leu Ile  Lys Phe Met His Ala  Asp Pro Ser Asn Val  Asn Leu Lys 
    1295                 1300                 1305             


Phe Gly  Asn Phe Lys Gly Asn  Arg Phe Gly Arg Lys  Asn Gly Asn 
    1310                 1315                 1320             


Ile Lys  Leu Ser Lys Thr Asp  Phe Ile Tyr Glu Ser  Pro Thr Gly 
    1325                 1330                 1335             


Leu Phe  Lys Ser Ile Lys His  Ile Asp 
    1340                 1345         


<210>  901
<211>  1347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  901

Met Lys Ser Glu Lys Lys Tyr Tyr Ile Gly Leu Asp Val Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Thr Asp Glu Phe Tyr Asn Ile Leu Arg Ala 
            20                  25                  30          


Lys Gly Lys Asp Leu Trp Gly Val Arg Leu Phe Glu Lys Ala Lys Thr 
        35                  40                  45              


Ala Ala Asp Thr Arg Thr Phe Arg Ser Gly Arg Arg Arg Asn Asp Arg 
    50                  55                  60                  


Lys Gly Met Arg Leu Gln Ile Leu Arg Glu Ile Phe Glu Asp Glu Ile 
65                  70                  75                  80  


Lys Lys Val Asp Lys Asp Phe Tyr Asp Arg Leu Asp Glu Ser Lys Phe 
                85                  90                  95      


Trp Ala Glu Asp Lys Lys Val Ser Gly Lys Tyr Ser Leu Phe Asn Asp 
            100                 105                 110         


Lys Asn Phe Ser Asp Lys Gln Tyr Phe Glu Lys Phe Pro Thr Ile Phe 
        115                 120                 125             


His Leu Arg Lys Tyr Leu Met Glu Glu His Gly Lys Val Asp Ile Arg 
    130                 135                 140                 


Tyr Tyr Phe Leu Ala Ile Asn Gln Met Met Lys Arg Arg Gly His Phe 
145                 150                 155                 160 


Leu Ile Asp Gly Gln Ile Ser His Val Thr Asp Asp Lys Pro Leu Lys 
                165                 170                 175     


Glu Gln Leu Ile Leu Leu Ile Asn Asp Leu Leu Lys Ile Glu Leu Glu 
            180                 185                 190         


Glu Glu Leu Met Asp Ser Ile Phe Glu Ile Leu Ala Asp Val Asn Glu 
        195                 200                 205             


Lys Arg Thr Asp Lys Lys Asn Asn Leu Lys Glu Leu Ile Lys Gly Gln 
    210                 215                 220                 


Asp Phe Asn Lys Gln Glu Gly Asn Ile Leu Asn Ser Ile Phe Glu Ser 
225                 230                 235                 240 


Ile Val Thr Gly Lys Ala Lys Ile Lys Asn Ile Ile Ser Asp Glu Asp 
                245                 250                 255     


Ile Leu Glu Lys Ile Lys Glu Asp Asn Lys Glu Asp Phe Val Leu Thr 
            260                 265                 270         


Gly Asp Ser Tyr Glu Glu Asn Leu Gln Tyr Phe Glu Glu Val Leu Gln 
        275                 280                 285             


Glu Asn Ile Thr Leu Phe Asn Thr Leu Lys Ser Thr Tyr Asp Phe Val 
    290                 295                 300                 


Ile Leu Gln Ser Ile Leu Lys Gly Lys Ser Thr Leu Ser Asp Ala Gln 
305                 310                 315                 320 


Val Glu Arg Tyr Asp Glu His Lys Lys Asp Leu Glu Ile Leu Lys Lys 
                325                 330                 335     


Val Ile Lys Lys Tyr Asp Glu Asp Gly Lys Leu Phe Lys Gln Val Phe 
            340                 345                 350         


Lys Glu Asp Asn Gly Asn Gly Tyr Val Ser Tyr Ile Gly Tyr Tyr Leu 
        355                 360                 365             


Asn Lys Asn Lys Lys Ile Thr Ala Lys Lys Lys Ile Ser Asn Ile Glu 
    370                 375                 380                 


Phe Thr Lys Tyr Val Lys Gly Ile Leu Glu Lys Gln Cys Asp Cys Glu 
385                 390                 395                 400 


Asp Glu Ala Val Lys Tyr Leu Leu Gly Lys Ile Glu Gln Glu Asn Phe 
                405                 410                 415     


Leu Leu Lys Gln Ile Ser Ser Ile Asn Ser Val Ile Pro His Gln Ile 
            420                 425                 430         


His Leu Phe Glu Leu Asp Lys Ile Leu Glu Asn Leu Ala Lys Asn Tyr 
        435                 440                 445             


Pro Ser Phe Asn Asn Lys Lys Glu Glu Phe Thr Lys Ile Glu Lys Ile 
    450                 455                 460                 


Arg Lys Thr Phe Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn 
465                 470                 475                 480 


Asp Tyr His Lys Asn Asn Gly Gly Asn Ala Trp Ile Phe Arg Asn Lys 
                485                 490                 495     


Gly Glu Lys Ile Arg Pro Trp Asn Phe Glu Lys Ile Val Asp Leu His 
            500                 505                 510         


Lys Ser Glu Glu Glu Phe Ile Lys Arg Met Leu Asn Gln Cys Thr Tyr 
        515                 520                 525             


Leu Pro Glu Glu Thr Val Leu Pro Lys Ser Ser Ile Leu Tyr Ser Glu 
    530                 535                 540                 


Tyr Met Val Leu Asn Glu Leu Asn Asn Leu Arg Ile Lys Gly Lys Pro 
545                 550                 555                 560 


Leu Asp Thr Asp Val Lys Leu Lys Leu Ile Glu Glu Leu Phe Lys Lys 
                565                 570                 575     


Lys Thr Lys Val Thr Leu Lys Ser Ile Arg Asp Tyr Met Ile Arg Asn 
            580                 585                 590         


Asn Phe Ala Asp Lys Glu Asp Phe Asp Asn Ser Glu Lys Asn Leu Glu 
        595                 600                 605             


Ile Val Ser Asn Met Lys Ser Tyr Ile Asp Phe Asn Asn Ile Leu Gly 
    610                 615                 620                 


Asp Lys Phe Glu Leu Glu Met Val Glu Asp Leu Ile Glu Lys Ile Thr 
625                 630                 635                 640 


Ile His Thr Gly Asn Lys Thr Leu Leu Lys Lys Tyr Ile Glu Glu Thr 
                645                 650                 655     


Tyr Pro Asp Leu Ser Ser Ser Gln Ile Gln Lys Ile Ile Asn Leu Lys 
            660                 665                 670         


Tyr Lys Asp Trp Gly Arg Leu Ser Arg Lys Leu Leu Asp Gly Ile Lys 
        675                 680                 685             


Gly Arg Asn Lys Thr Asp Asp Val Asn Thr Val Ile Asn Phe Met Arg 
    690                 695                 700                 


Asn Ser Ser Asp Asn Leu Met Gln Ile Ile Glu Gly Lys Asn Tyr Ser 
705                 710                 715                 720 


Phe Asn Glu Tyr Ile Asp Asn Leu Arg Lys Lys Tyr Ile Pro Gln Glu 
                725                 730                 735     


Ile Ser Tyr Glu Val Val Glu Asn Leu Tyr Val Ser Pro Ser Val Lys 
            740                 745                 750         


Lys Met Ile Trp Gln Val Ile Arg Val Thr Glu Glu Ile Thr Lys Val 
        755                 760                 765             


Met Gly Tyr Asp Pro Asp Lys Ile Phe Ile Glu Met Ala Lys Ser Glu 
    770                 775                 780                 


Glu Glu Lys Lys Thr Thr Ile Ser Arg Lys Asn Lys Leu Leu Asp Leu 
785                 790                 795                 800 


Tyr Lys Ala Ile Lys Lys Asp Glu Arg Asp Ser Gln Tyr Glu Lys Leu 
                805                 810                 815     


Leu Thr Gly Leu Asn Lys Leu Asp Glu Ser Glu Leu Arg Ser Lys Lys 
            820                 825                 830         


Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Asp Met Tyr Thr Gly Glu 
        835                 840                 845             


Lys Ile Asp Leu Asp Lys Leu Phe Asp Ser Thr His Tyr Asp Lys Asp 
    850                 855                 860                 


His Ile Ile Pro Gln Ser Met Lys Lys Asp Asp Ser Ile Ile Asn Asn 
865                 870                 875                 880 


Leu Val Leu Val Asn Lys Asn Ala Asn Gln Thr Thr Lys Gly Asn Ile 
                885                 890                 895     


Tyr Pro Val Pro Ser Ser Ile Arg Asn Asn Pro Asp Ile Tyr Asn Tyr 
            900                 905                 910         


Trp Lys Tyr Leu Met Glu Lys Glu Phe Ile Ser Lys Glu Lys Tyr Asn 
        915                 920                 925             


Arg Leu Ile Arg Asn Thr Pro Leu Thr Asn Glu Glu Leu Gly Val Phe 
    930                 935                 940                 


Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala Ile Lys 
945                 950                 955                 960 


Glu Leu Phe Glu Lys Phe Tyr Gln Lys Ser Lys Ile Ile Pro Val Lys 
                965                 970                 975     


Ala Ser Leu Ala Ser Asp Leu Arg Lys Asp Met Asn Thr Leu Lys Ser 
            980                 985                 990         


Arg Glu Val Asn Asp Leu His His  Ala His Asp Ala Phe  Leu Asn Ile 
        995                 1000                 1005             


Val Ala  Gly Asp Val Trp Asn  Arg Glu Phe Thr Ser  Asn Pro Ile 
    1010                 1015                 1020             


Asn Tyr  Val Asn Glu Asn Arg  Glu Gly Asp Lys Val  Lys Tyr Ser 
    1025                 1030                 1035             


Leu Ser  Lys Tyr Phe Thr Arg  Pro Arg Lys Ser Lys  Gly Lys Val 
    1040                 1045                 1050             


Ile Trp  Thr Pro Glu Lys Gly  Arg Lys Leu Ile Val  Asp Thr Leu 
    1055                 1060                 1065             


Asn Lys  Pro Ser Val Leu Ile  Ser Asn Glu Ser His  Val Lys Lys 
    1070                 1075                 1080             


Gly Glu  Leu Phe Asn Ala Thr  Ile Ser Gly Lys Lys  Asp Tyr Lys 
    1085                 1090                 1095             


Lys Gly  Lys Ile Tyr Leu Pro  Leu Lys Lys Asp Asp  Arg Leu Lys 
    1100                 1105                 1110             


Asp Val  Ser Lys Tyr Gly Gly  Tyr Lys Ala Ile Asn  Gly Ala Phe 
    1115                 1120                 1125             


Phe Phe  Leu Val Glu His Thr  Lys Ser Lys Lys Arg  Ile Arg Ser 
    1130                 1135                 1140             


Ile Glu  Leu Phe Pro Leu His  Leu Leu Ser Lys Phe  Tyr Glu Asp 
    1145                 1150                 1155             


Lys Asn  Thr Val Leu Asp Tyr  Ala Ile Asn Val Leu  Gln Leu Gln 
    1160                 1165                 1170             


Asp Pro  Lys Ile Ile Ile Asp  Lys Ile Asn Tyr Arg  Thr Glu Ile 
    1175                 1180                 1185             


Ile Ile  Asp Asn Phe Ser Tyr  Leu Ile Ser Thr Lys  Ser Asn Asp 
    1190                 1195                 1200             


Gly Ser  Ile Thr Val Lys Pro  Asn Glu Gln Met Tyr  Trp Arg Val 
    1205                 1210                 1215             


Asp Glu  Ile Ser Asn Leu Lys  Lys Ile Glu Asn Lys  Tyr Lys Lys 
    1220                 1225                 1230             


Asp Ala  Ile Leu Thr Glu Glu  Asp Arg Lys Ile Met  Glu Ser Tyr 
    1235                 1240                 1245             


Ile Asp  Lys Ile Tyr Gln Gln  Phe Lys Ala Gly Lys  Tyr Lys Asn 
    1250                 1255                 1260             


Arg Arg  Thr Thr Asp Thr Ile  Ile Glu Lys Tyr Glu  Ile Ile Asp 
    1265                 1270                 1275             


Leu Asp  Thr Leu Asp Asn Lys  Gln Leu Tyr Gln Leu  Leu Val Ala 
    1280                 1285                 1290             


Phe Ile  Ser Leu Ser Tyr Lys  Thr Ser Asn Asn Ala  Val Asp Phe 
    1295                 1300                 1305             


Thr Val  Ile Gly Leu Gly Thr  Glu Cys Gly Lys Pro  Arg Ile Thr 
    1310                 1315                 1320             


Asn Leu  Pro Asp Asn Thr Tyr  Leu Val Tyr Lys Ser  Ile Thr Gly 
    1325                 1330                 1335             


Ile Tyr  Glu Lys Arg Ile Arg  Ile Lys 
    1340                 1345         


<210>  902
<211>  1348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  902

Met Lys Lys Pro Tyr Thr Ile Ala Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Val Val Val Thr Asp Asp Tyr Arg Val Pro Thr Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Glu Arg Lys Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Asp Thr Ala Glu Gly Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Lys Glu Ile Phe Thr Glu Glu Met Ala Lys Val Asp Asp Gly 
                85                  90                  95      


Phe Phe Gln Arg Leu Glu Asp Ser Phe Tyr Val Leu Glu Asp Lys Glu 
            100                 105                 110         


Gly Asn Lys His Pro Ile Phe Ala Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             


His Lys Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Val Asp 
    130                 135                 140                 


Asn Pro Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Val Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Thr Leu Ser Ser 
                165                 170                 175     


Lys Asn Asn Asn Leu Gln Lys Ser Phe Asp His Leu Val Asp Thr Tyr 
            180                 185                 190         


Asn Leu Leu Phe Glu Glu Gln Arg Leu Leu Thr Glu Gly Ile Asn Ala 
        195                 200                 205             


Lys Glu Leu Leu Ser Ala Ala Leu Ser Lys Ser Lys Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ser Leu Ile Pro Gly Gln Lys Lys Thr Gly Ile Phe Gly Asn 
225                 230                 235                 240 


Ile Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ala Asn Phe 
                245                 250                 255     


Gly Leu Ser Lys Asp Val Lys Leu Gln Leu Ala Lys Asp Thr Tyr Ala 
            260                 265                 270         


Asp Asp Leu Asp Ser Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Thr Glu Ser Asp Glu Ile Thr Arg Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Val Lys Arg Tyr Arg Glu His His Lys Asp Leu Val Thr Leu Lys 
                325                 330                 335     


Thr Leu Ile Lys Asp Gln Leu Pro Glu Lys Tyr Gln Glu Ile Phe Leu 
            340                 345                 350         


Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Gln Val Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Tyr Leu Lys Pro Ile Leu Ala Arg Leu Asp 
    370                 375                 380                 


Gly Ser Glu Pro Leu Leu Leu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Val Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Lys Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly His Ser Arg Phe Ala Trp 
    450                 455                 460                 


Val Lys Arg Lys Phe Asp Gly Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Ile Val Asp Glu Glu Ala Ser Ala Gln Ile Phe Ile Glu Lys Met Thr 
                485                 490                 495     


Lys Asn Asp Leu Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Ala Thr Glu Gly Met Thr Arg Pro Gln Phe Leu Ser Ala Asp Gln 
    530                 535                 540                 


Lys Gln Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asn Tyr Phe Lys Lys Ile Glu Cys Trp Asp 
                565                 570                 575     


Ser Val Glu Ile Thr Gly Val Glu Asp Ser Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Gln Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Pro Asp Asn Gln Lys Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Lys Lys Met Ile Ser Lys Arg Leu Asp Gln Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Lys Val Val Leu Asn Lys Leu Glu Arg His His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Gly Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Ser Glu Leu Ser Phe 
    690                 695                 700                 


Ile Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Lys Thr Glu Tyr Ser 
705                 710                 715                 720 


Lys Asp Leu Val Gly Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Ser Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Ile Met Gly 
            740                 745                 750         


Tyr Leu Pro Gln Gln Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Gln Gly Ile Lys Asn Ala Arg Gln Arg Met Arg Lys Leu Glu 
    770                 775                 780                 


Glu Thr Ala Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Asp Asn Ser Gln Leu Gln Asn Asp Lys Arg Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Lys Asp Met Tyr Thr Gly Asp Asp Leu Asp Ile Asp Tyr Leu 
            820                 825                 830         


Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asn 
        835                 840                 845             


Asn Ser Ile Asp Asn Lys Val Leu Thr Ser Gln Gly Ala Asn Arg Gly 
    850                 855                 860                 


Lys Leu Asp Asn Val Pro Ser Glu Ala Ile Val Arg Lys Met Lys Gly 
865                 870                 875                 880 


Tyr Trp Gln Ser Leu Leu Arg Ala Gly Ala Ile Ser Lys Gln Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Gln Val Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Phe Asp Asp 
    930                 935                 940                 


His Asn Lys Arg Ile Arg Lys Val His Ile Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Glu Phe Gly Leu Tyr Lys Ile Arg Asp 
                965                 970                 975     


Ile Asn His Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Ala Lys Ala Ile Leu Gly Lys Tyr  Pro Gln Leu Ala Pro  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Pro Lys Tyr  Asn Ser Phe Lys Glu  Arg Gln Lys 
    1010                 1015                 1020             


Ala Thr  Gln Lys Met Leu Phe  Tyr Ser Asn Ile Leu  Lys Phe Phe 
    1025                 1030                 1035             


Lys Asp  Gln Glu Ser Leu His  Val Asn Ser Asp Gly  Glu Glu Ile 
    1040                 1045                 1050             


Trp Asn  Ala Asn Lys His Leu  Pro Ile Ile Lys Asn  Val Leu Ser 
    1055                 1060                 1065             


Ile Pro  Gln Val Asn Ile Val  Lys Lys Thr Glu Val  Gln Thr Gly 
    1070                 1075                 1080             


Gly Phe  Tyr Lys Glu Ser Ile  Leu Ser Lys Gly Asn  Ser Asp Lys 
    1085                 1090                 1095             


Leu Ile  Pro Arg Lys Asn Asn  Trp Asp Thr Arg Lys  Tyr Gly Gly 
    1100                 1105                 1110             


Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val Leu Val  Ile Ala Lys 
    1115                 1120                 1125             


Met Glu  Lys Gly Lys Ala Lys  Val Leu Lys Pro Val  Lys Glu Met 
    1130                 1135                 1140             


Val Gly  Ile Thr Ile Met Glu  Arg Thr Ala Phe Glu  Glu Asn Pro 
    1145                 1150                 1155             


Val Val  Phe Leu Glu Ala Arg  Gly Tyr Arg Glu Ile  Gln Glu His 
    1160                 1165                 1170             


Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn 
    1175                 1180                 1185             


Gly Arg  Arg Arg Leu Leu Ala  Ser Ala Ser Glu Leu  Gln Lys Gly 
    1190                 1195                 1200             


Asn Glu  Leu Phe Leu Pro Val  Asp Tyr Met Thr Phe  Leu Tyr Leu 
    1205                 1210                 1215             


Ala Ala  His Tyr His Glu Leu  Thr Gly Ser Ser Glu  Asp Val Leu 
    1220                 1225                 1230             


Arg Lys  Lys Tyr Phe Val Asp  Arg His Leu His Tyr  Phe Asp Asp 
    1235                 1240                 1245             


Ile Ile  Gln Met Ile Asn Asp  Phe Ala Glu Arg His  Ile Leu Ala 
    1250                 1255                 1260             


Ser Ser  Asn Leu Glu Lys Ile  Asn His Thr Tyr His  Asn Asn Ser 
    1265                 1270                 1275             


Asp Leu  Pro Val Asn Glu Arg  Ala Glu Asn Ile Ile  Asn Val Phe 
    1280                 1285                 1290             


Thr Phe  Val Ala Leu Gly Ala  Pro Ala Ala Phe Lys  Phe Phe Asp 
    1295                 1300                 1305             


Ala Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser Thr Lys  Glu Val Leu 
    1310                 1315                 1320             


Asn Ala  Thr Leu Ile His Gln  Ser Val Thr Gly Leu  Tyr Glu Thr 
    1325                 1330                 1335             


Arg Ile  Asp Leu Ser Gln Leu  Gly Glu Asn 
    1340                 1345             


<210>  903
<211>  1348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  903

Met Lys Ser Glu Lys Lys Tyr Tyr Ile Gly Leu Asp Val Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Thr Asp Glu Phe Tyr Asn Ile Leu Arg Ala 
            20                  25                  30          


Lys Gly Lys Asp Leu Trp Gly Val Arg Leu Phe Glu Lys Ala Lys Thr 
        35                  40                  45              


Ala Ala Asp Thr Arg Thr Phe Arg Ser Gly Arg Arg Arg Asn Asp Arg 
    50                  55                  60                  


Lys Gly Val Arg Leu Gln Ile Leu Arg Glu Ile Phe Glu Asp Glu Ile 
65                  70                  75                  80  


Lys Lys Val Asp Lys Asp Phe Tyr Asp Arg Leu Asp Glu Ser Lys Phe 
                85                  90                  95      


Trp Ala Glu Asp Lys Lys Val Ser Gly Lys Tyr Ser Leu Phe Asn Asp 
            100                 105                 110         


Lys Asn Phe Ser Asp Lys Gln Tyr Phe Glu Lys Phe Pro Thr Ile Phe 
        115                 120                 125             


His Leu Arg Lys Tyr Leu Met Glu Glu His Gly Lys Val Asp Ile Arg 
    130                 135                 140                 


Tyr Tyr Phe Leu Ala Ile Asn Gln Met Met Lys Arg Arg Gly His Phe 
145                 150                 155                 160 


Leu Ile Asp Gly Gln Ile Ser His Val Thr Asp Asp Lys Pro Leu Lys 
                165                 170                 175     


Gln Gln Leu Ile Leu Leu Ile Asn Asp Leu Leu Lys Ile Glu Leu Glu 
            180                 185                 190         


Glu Glu Phe Met Asp Ser Ile Phe Glu Ile Leu Ala Asp Val Asn Glu 
        195                 200                 205             


Lys Lys Thr Glu Lys Lys Asn Ile Leu Lys Glu Leu Ile Asn Ala Gln 
    210                 215                 220                 


Asp Phe Asn Lys Gln Glu Gly Lys Ile Leu Tyr Ser Ile Phe Glu Ser 
225                 230                 235                 240 


Ile Val Thr Gly Lys Ala Lys Ile Lys Asn Ile Ile Ser Asp Glu Asp 
                245                 250                 255     


Ile Leu Glu Lys Ile Lys Glu Asp Asn Lys Glu Asp Phe Val Leu Thr 
            260                 265                 270         


Gly Asp Ser Tyr Glu Glu Asn Leu Gln Tyr Phe Glu Glu Val Leu Gln 
        275                 280                 285             


Glu Asn Ile Thr Leu Phe Asn Thr Leu Lys Ser Ala Tyr Asp Phe Val 
    290                 295                 300                 


Ile Leu Gln Ser Ile Leu Lys Gly Lys Ser Thr Leu Ser Asp Ala Gln 
305                 310                 315                 320 


Val Glu Arg Tyr Asp Glu His Lys Lys Asp Leu Glu Ile Leu Lys Lys 
                325                 330                 335     


Val Ile Lys Lys Tyr Asp Glu Asp Gly Lys Leu Phe Lys Gln Val Phe 
            340                 345                 350         


Lys Glu Asp Asn Gly Asn Gly Tyr Val Ser Tyr Ile Gly Tyr Tyr Leu 
        355                 360                 365             


Asn Lys Asn Lys Lys Ile Thr Ala Lys Lys Lys Ile Ser Asn Ile Glu 
    370                 375                 380                 


Phe Thr Lys Tyr Val Lys Gly Ile Leu Glu Lys Gln Cys Asp Cys Glu 
385                 390                 395                 400 


Asp Glu Asp Val Lys Tyr Leu Leu Gly Lys Ile Glu Gln Glu Asn Phe 
                405                 410                 415     


Leu Leu Lys Gln Ile Ser Ser Ile Asn Ser Val Ile Pro His Gln Ile 
            420                 425                 430         


His Leu Phe Glu Leu Asp Lys Ile Leu Glu Asn Leu Ala Lys Tyr Tyr 
        435                 440                 445             


Pro Ser Phe Asn Asn Lys Lys Glu Glu Phe Thr Lys Ile Glu Lys Ile 
    450                 455                 460                 


Arg Lys Thr Phe Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn 
465                 470                 475                 480 


Asp Tyr His Lys Asn Asn Gly Gly Asn Ala Trp Ile Phe Arg Asn Lys 
                485                 490                 495     


Gly Glu Lys Ile Arg Pro Trp Asn Phe Glu Lys Ile Val Asp Leu His 
            500                 505                 510         


Lys Ser Glu Glu Glu Phe Ile Lys Arg Met Leu Asn Gln Cys Thr Tyr 
        515                 520                 525             


Leu Pro Glu Glu Thr Val Leu Pro Lys Ser Ser Ile Leu Tyr Ser Glu 
    530                 535                 540                 


Tyr Met Val Leu Asn Glu Leu Asn Asn Leu Arg Ile Asn Gly Lys Pro 
545                 550                 555                 560 


Ile Asp Thr Asp Val Lys Leu Lys Leu Ile Glu Glu Leu Phe Lys Lys 
                565                 570                 575     


Lys Thr Lys Val Thr Leu Lys Ser Ile Arg Asp Tyr Met Ile Arg Asn 
            580                 585                 590         


Asn Phe Ala Asp Lys Glu Asp Phe Asp Asn Ser Glu Lys Asn Leu Glu 
        595                 600                 605             


Ile Val Ser Asn Met Lys Ser Tyr Ile Asp Phe Asn Asn Ile Leu Gly 
    610                 615                 620                 


Asp Lys Phe Glu Leu Glu Met Val Glu Asp Leu Ile Glu Lys Ile Thr 
625                 630                 635                 640 


Ile His Thr Gly Asn Lys Lys Leu Leu Lys Lys Tyr Ile Glu Glu Thr 
                645                 650                 655     


Tyr Pro Asp Leu Ser Ser Ser Gln Ile Gln Lys Ile Ile Asn Leu Lys 
            660                 665                 670         


Tyr Lys Asp Trp Gly Arg Leu Ser Arg Lys Leu Leu Asp Gly Ile Thr 
        675                 680                 685             


Gly Thr Asn Ile Glu Thr Gly Glu Ile Asp Thr Val Ile Asn Phe Leu 
    690                 695                 700                 


Arg Asn Ser Ser Asp Asn Leu Met Gln Ile Ile Gly Ser Gln Asn Tyr 
705                 710                 715                 720 


Ser Phe Asn Asn Asp Ile Asp Lys Leu Arg Lys Lys Tyr Ile Pro Gln 
                725                 730                 735     


Glu Ile Ser Tyr Glu Val Val Glu Asn Leu Tyr Val Ser Pro Ser Val 
            740                 745                 750         


Lys Lys Met Ile Trp Gln Val Ile Arg Ile Thr Glu Glu Ile Thr Lys 
        755                 760                 765             


Val Met Gly Tyr Asp Pro Asp Lys Ile Phe Ile Glu Met Ala Lys Ser 
    770                 775                 780                 


Glu Glu Gly Lys Lys Thr Thr Ile Ser Arg Lys Asn Lys Leu Leu Ala 
785                 790                 795                 800 


Leu Tyr Lys Ala Ile Lys Lys Asp Glu Arg Asp Ser Gln Tyr Glu Lys 
                805                 810                 815     


Leu Leu Thr Gly Leu Asn Lys Leu Asp Asp Ser Asp Leu Arg Ser Arg 
            820                 825                 830         


Lys Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Asp Met Tyr Thr Gly 
        835                 840                 845             


Glu Lys Ile Asp Leu Asp Lys Leu Phe Asp Ser Thr His Tyr Asp Lys 
    850                 855                 860                 


Asp His Ile Ile Pro Gln Ser Met Lys Lys Asp Asp Ser Ile Ile Asn 
865                 870                 875                 880 


Asn Leu Val Leu Val Asn Lys Asn Ala Asn Gln Thr Thr Lys Gly Asn 
                885                 890                 895     


Ile Tyr Pro Val Pro Ser Ser Ile Arg Asn Asn Pro Lys Ile Tyr Asn 
            900                 905                 910         


Tyr Trp Lys Tyr Leu Met Glu Lys Glu Phe Ile Ser Lys Glu Lys Tyr 
        915                 920                 925             


Asn Arg Leu Ile Arg Asn Thr Pro Leu Thr Asn Glu Glu Leu Gly Gly 
    930                 935                 940                 


Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala Ile 
945                 950                 955                 960 


Lys Glu Leu Phe Glu Lys Phe Tyr Lys Lys Ser Lys Ile Ile Pro Val 
                965                 970                 975     


Lys Ala Ile Leu Ala Ser Asp Leu Arg Lys Asp Met Asn Thr Leu Lys 
            980                 985                 990         


Ser Arg Glu Val Asn Asp Leu His  His Ala His Asp Ala  Phe Leu Asn 
        995                 1000                 1005             


Ile Val  Ala Gly Asp Val Trp  Asn Arg Glu Phe Thr  Ser Asn Pro 
    1010                 1015                 1020             


Ile Asn  Tyr Val Lys Glu Asn  Arg Glu Gly Asp Lys  Val Lys Tyr 
    1025                 1030                 1035             


Ser Leu  Ser Lys Tyr Phe Thr  Arg Pro Arg Lys Ser  Lys Gly Lys 
    1040                 1045                 1050             


Val Ile  Trp Thr Pro Glu Lys  Gly Arg Lys Leu Ile  Val Asp Thr 
    1055                 1060                 1065             


Leu Asn  Lys Pro Ser Val Leu  Ile Ser Asn Glu Ser  His Val Lys 
    1070                 1075                 1080             


Lys Gly  Glu Leu Phe Asn Ala  Thr Ile Ala Gly Lys  Lys Asp Tyr 
    1085                 1090                 1095             


Lys Lys  Gly Lys Ile Tyr Leu  Pro Leu Lys Lys Asp  Asp Arg Leu 
    1100                 1105                 1110             


Gln Asp  Val Ser Lys Tyr Gly  Gly Tyr Lys Ala Ile  Asn Gly Ala 
    1115                 1120                 1125             


Phe Phe  Phe Leu Val Glu His  Thr Lys Ser Lys Lys  Arg Ile Arg 
    1130                 1135                 1140             


Ser Ile  Glu Leu Phe Pro Leu  His Leu Leu Ser Lys  Phe Tyr Glu 
    1145                 1150                 1155             


Asp Lys  Asn Thr Val Leu Asp  Tyr Ala Ile Asn Val  Leu Gln Leu 
    1160                 1165                 1170             


Gln Asp  Pro Lys Ile Ile Ile  Asp Lys Ile Asn Tyr  Arg Thr Glu 
    1175                 1180                 1185             


Ile Ile  Ile Asp Asn Phe Ser  Tyr Leu Ile Ser Thr  Lys Ser Asn 
    1190                 1195                 1200             


Asp Gly  Ser Ile Thr Val Lys  Pro Asn Glu Gln Met  Tyr Trp Arg 
    1205                 1210                 1215             


Val Asp  Glu Ile Ser Asn Leu  Lys Lys Ile Glu Asn  Lys Tyr Lys 
    1220                 1225                 1230             


Lys Asp  Ala Ile Leu Thr Glu  Glu Asp Arg Lys Ile  Met Glu Ser 
    1235                 1240                 1245             


Tyr Ile  Asp Lys Ile Tyr Gln  Gln Phe Lys Ala Gly  Lys Tyr Lys 
    1250                 1255                 1260             


Asn Arg  Arg Thr Thr Asp Thr  Ile Ile Glu Lys Tyr  Glu Ile Ile 
    1265                 1270                 1275             


Asp Leu  Asp Thr Leu Asp Asn  Lys Gln Leu Tyr Gln  Leu Leu Val 
    1280                 1285                 1290             


Ala Phe  Ile Ser Leu Ser Tyr  Lys Thr Ser Asn Asn  Ala Val Asp 
    1295                 1300                 1305             


Phe Thr  Val Ile Gly Leu Gly  Thr Glu Cys Gly Lys  Pro Arg Ile 
    1310                 1315                 1320             


Thr Asn  Leu Pro Asp Asn Thr  Tyr Leu Val Tyr Lys  Ser Ile Thr 
    1325                 1330                 1335             


Gly Ile  Tyr Glu Lys Arg Ile  Arg Ile Lys 
    1340                 1345             


<210>  904
<211>  1348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  904

Met Lys Ser Glu Lys Lys Tyr Tyr Ile Gly Leu Asp Val Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Thr Asp Glu Phe Tyr Asn Ile Leu Arg Ala 
            20                  25                  30          


Lys Gly Lys Asp Leu Trp Gly Val Arg Leu Phe Glu Lys Ala Asp Thr 
        35                  40                  45              


Ala Ala Asn Thr Arg Ile Phe Arg Ser Gly Arg Arg Arg Asn Asp Arg 
    50                  55                  60                  


Lys Gly Met Arg Leu Gln Ile Leu Arg Glu Ile Phe Glu Asp Glu Ile 
65                  70                  75                  80  


Lys Lys Val Asp Lys Asp Phe Tyr Asp Arg Leu Asp Glu Ser Lys Phe 
                85                  90                  95      


Trp Ala Glu Asp Lys Lys Val Ser Gly Lys Tyr Ser Leu Phe Asn Asp 
            100                 105                 110         


Lys Asn Phe Ser Asp Lys Gln Tyr Phe Glu Lys Phe Pro Thr Ile Phe 
        115                 120                 125             


His Leu Arg Lys Tyr Leu Met Glu Glu His Gly Lys Val Asp Ile Arg 
    130                 135                 140                 


Tyr Tyr Phe Leu Ala Ile Asn Gln Met Met Lys Arg Arg Gly His Phe 
145                 150                 155                 160 


Leu Ile Asp Gly Gln Ile Ser His Val Thr Asp Asp Lys Pro Leu Lys 
                165                 170                 175     


Glu Gln Leu Ile Leu Leu Ile Asn Asp Leu Leu Lys Ile Glu Leu Glu 
            180                 185                 190         


Glu Glu Leu Met Asp Ser Ile Phe Glu Ile Leu Ala Asp Val Asn Glu 
        195                 200                 205             


Lys Arg Thr Asp Lys Lys Asn Asn Leu Lys Glu Leu Ile Lys Gly Gln 
    210                 215                 220                 


Asp Phe Asn Lys Gln Glu Gly Asn Ile Leu Asn Ser Ile Phe Glu Ser 
225                 230                 235                 240 


Ile Val Thr Gly Lys Ala Lys Ile Lys Asn Ile Ile Ser Asp Glu Asp 
                245                 250                 255     


Ile Leu Glu Lys Ile Lys Glu Asp Asn Lys Glu Asp Phe Val Leu Thr 
            260                 265                 270         


Gly Asp Ser Tyr Glu Glu Asn Leu Gln Tyr Phe Glu Glu Val Leu Gln 
        275                 280                 285             


Glu Asn Ile Thr Leu Phe Asn Thr Leu Lys Ser Thr Tyr Asp Phe Leu 
    290                 295                 300                 


Ile Leu Gln Ser Ile Leu Lys Gly Lys Ser Thr Leu Ser Asp Ala Gln 
305                 310                 315                 320 


Val Glu Arg Tyr Asp Glu His Lys Lys Asp Leu Glu Ile Leu Lys Lys 
                325                 330                 335     


Val Ile Lys Lys Tyr Asp Glu Asp Gly Lys Leu Phe Lys Gln Val Phe 
            340                 345                 350         


Lys Glu Asp Asn Gly Asn Gly Tyr Val Ser Tyr Ile Gly Tyr Tyr Leu 
        355                 360                 365             


Asn Lys Asn Lys Lys Ile Thr Ala Lys Lys Lys Ile Ser Asn Ile Glu 
    370                 375                 380                 


Phe Thr Lys Tyr Val Lys Gly Ile Leu Glu Lys Gln Cys Asp Cys Glu 
385                 390                 395                 400 


Asp Glu Asp Val Lys Tyr Leu Leu Gly Lys Ile Glu Gln Glu Asn Phe 
                405                 410                 415     


Leu Leu Lys Gln Ile Ser Ser Ile Asn Ser Val Ile Pro His Gln Ile 
            420                 425                 430         


His Leu Phe Glu Leu Asp Lys Ile Leu Glu Asn Leu Ala Lys Asn Tyr 
        435                 440                 445             


Pro Ser Phe Asn Asn Lys Lys Glu Glu Phe Thr Lys Ile Glu Lys Ile 
    450                 455                 460                 


Arg Lys Thr Phe Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn 
465                 470                 475                 480 


Asp Tyr His Lys Asn Asn Gly Gly Asn Ala Trp Ile Phe Arg Asn Lys 
                485                 490                 495     


Gly Glu Lys Ile Arg Pro Trp Asn Phe Glu Lys Ile Val Asp Leu His 
            500                 505                 510         


Lys Ser Glu Glu Glu Phe Ile Lys Arg Met Leu Asn Gln Cys Thr Tyr 
        515                 520                 525             


Leu Pro Glu Glu Thr Val Leu Pro Lys Ser Ser Ile Leu Tyr Ser Glu 
    530                 535                 540                 


Tyr Met Val Leu Asn Glu Leu Asn Asn Leu Arg Ile Asn Gly Lys Pro 
545                 550                 555                 560 


Leu Asp Thr Asp Val Lys Leu Lys Leu Ile Glu Glu Leu Phe Lys Lys 
                565                 570                 575     


Lys Thr Lys Val Thr Leu Lys Ser Ile Arg Asp Tyr Met Val Arg Asn 
            580                 585                 590         


Asn Phe Ala Asp Lys Glu Asp Phe Asp Asn Ser Glu Lys Asn Leu Glu 
        595                 600                 605             


Ile Ala Ser Asn Met Lys Ser Tyr Ile Asp Phe Asn Asn Ile Leu Glu 
    610                 615                 620                 


Asp Lys Phe Asp Val Glu Met Val Glu Asp Leu Ile Glu Lys Ile Thr 
625                 630                 635                 640 


Ile His Thr Gly Asn Lys Lys Leu Leu Lys Lys Tyr Ile Glu Glu Thr 
                645                 650                 655     


Tyr Pro Asp Leu Ser Ser Ser Gln Ile Gln Lys Ile Ile Asn Leu Lys 
            660                 665                 670         


Tyr Lys Asp Trp Gly Arg Leu Ser Arg Lys Leu Leu Asp Gly Ile Lys 
        675                 680                 685             


Gly Thr Lys Lys Glu Thr Glu Lys Thr Asp Thr Val Ile Asn Phe Leu 
    690                 695                 700                 


Arg Asn Ser Ser Asp Asn Leu Met Gln Ile Ile Gly Ser Gln Asn Tyr 
705                 710                 715                 720 


Ser Phe Asn Glu Tyr Ile Asp Lys Leu Arg Lys Lys Tyr Ile Pro Gln 
                725                 730                 735     


Glu Ile Ser Tyr Glu Val Val Glu Asn Leu Tyr Val Ser Pro Ser Val 
            740                 745                 750         


Lys Lys Met Ile Trp Gln Val Ile Arg Val Thr Glu Glu Ile Thr Lys 
        755                 760                 765             


Val Met Gly Tyr Asp Pro Asp Lys Ile Phe Ile Glu Met Ala Lys Ser 
    770                 775                 780                 


Glu Glu Glu Lys Lys Thr Thr Ile Ser Arg Lys Asn Lys Leu Leu Asp 
785                 790                 795                 800 


Leu Tyr Lys Ala Ile Lys Lys Asp Glu Arg Asp Ser Gln Tyr Glu Lys 
                805                 810                 815     


Leu Leu Thr Gly Leu Asn Lys Leu Asp Asp Ser Asp Leu Arg Ser Arg 
            820                 825                 830         


Lys Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Asp Met Tyr Thr Gly 
        835                 840                 845             


Glu Lys Ile Asp Leu Asp Lys Leu Phe Asp Ser Thr His Tyr Asp Lys 
    850                 855                 860                 


Asp His Ile Ile Pro Gln Ser Met Lys Lys Asp Asp Ser Ile Ile Asn 
865                 870                 875                 880 


Asn Leu Val Leu Val Asn Lys Asn Ala Asn Gln Thr Thr Lys Gly Asn 
                885                 890                 895     


Ile Tyr Pro Val Pro Ser Ser Ile Arg Asn Asn Pro Lys Ile Tyr Asn 
            900                 905                 910         


Tyr Trp Lys Tyr Leu Met Glu Lys Glu Phe Ile Ser Lys Glu Lys Tyr 
        915                 920                 925             


Asn Arg Leu Ile Arg Asn Thr Pro Leu Thr Asn Glu Glu Leu Gly Gly 
    930                 935                 940                 


Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala Ile 
945                 950                 955                 960 


Lys Glu Leu Phe Glu Lys Phe Tyr Gln Lys Ser Lys Ile Ile Pro Val 
                965                 970                 975     


Lys Ala Ser Leu Ala Ser Asp Leu Arg Lys Asp Met Asn Thr Leu Lys 
            980                 985                 990         


Ser Arg Glu Val Asn Asp Leu His  His Ala His Asp Ala  Phe Leu Asn 
        995                 1000                 1005             


Ile Val  Ala Gly Asp Val Trp  Asn Arg Glu Phe Thr  Ser Asn Pro 
    1010                 1015                 1020             


Ile Asn  Tyr Val Lys Glu Asn  Arg Glu Gly Asp Lys  Val Lys Tyr 
    1025                 1030                 1035             


Ser Leu  Ser Lys Asp Phe Thr  Arg Pro Arg Lys Ser  Lys Gly Lys 
    1040                 1045                 1050             


Val Ile  Trp Thr Pro Glu Lys  Gly Arg Lys Leu Ile  Val Asp Thr 
    1055                 1060                 1065             


Leu Asn  Lys Pro Ser Val Leu  Ile Ser Asn Glu Ser  His Val Lys 
    1070                 1075                 1080             


Lys Gly  Glu Leu Phe Asn Ala  Thr Ile Ala Gly Lys  Lys Asp Tyr 
    1085                 1090                 1095             


Lys Lys  Gly Lys Ile Tyr Leu  Pro Leu Lys Lys Asp  Asp Arg Leu 
    1100                 1105                 1110             


Gln Asp  Val Ser Lys Tyr Gly  Gly Tyr Lys Ala Ile  Asn Gly Ala 
    1115                 1120                 1125             


Phe Phe  Phe Leu Val Glu His  Thr Lys Ser Lys Lys  Arg Ile Arg 
    1130                 1135                 1140             


Ser Ile  Glu Leu Phe Pro Leu  His Leu Leu Ser Lys  Phe Tyr Glu 
    1145                 1150                 1155             


Asp Lys  Asn Thr Val Leu Asp  Tyr Ala Ile Asn Val  Leu Gln Leu 
    1160                 1165                 1170             


Gln Asp  Pro Lys Ile Ile Ile  Asp Lys Ile Asn Tyr  Arg Thr Glu 
    1175                 1180                 1185             


Ile Ile  Ile Asp Asn Phe Ser  Tyr Leu Ile Ser Thr  Lys Ser Asn 
    1190                 1195                 1200             


Asp Gly  Ser Ile Thr Val Lys  Pro Asn Glu Gln Met  Tyr Trp Arg 
    1205                 1210                 1215             


Val Asp  Glu Ile Ser Asn Leu  Lys Lys Ile Glu Asn  Lys Tyr Lys 
    1220                 1225                 1230             


Lys Asp  Ala Ile Leu Thr Glu  Glu Asp Arg Lys Ile  Met Glu Ser 
    1235                 1240                 1245             


Tyr Ile  Asp Lys Ile Tyr Gln  Gln Phe Lys Ala Gly  Lys Tyr Lys 
    1250                 1255                 1260             


Asn Arg  Arg Thr Thr Asp Thr  Ile Ile Glu Lys Tyr  Glu Ile Ile 
    1265                 1270                 1275             


Asp Leu  Asp Thr Leu Asp Asn  Lys Gln Leu Tyr Gln  Leu Leu Val 
    1280                 1285                 1290             


Ala Phe  Ile Ser Leu Ser Tyr  Lys Thr Ser Asn Asn  Ala Val Asp 
    1295                 1300                 1305             


Phe Thr  Val Ile Gly Leu Gly  Thr Glu Cys Gly Lys  Pro Arg Ile 
    1310                 1315                 1320             


Thr Asn  Leu Pro Asp Asn Thr  Tyr Leu Val Tyr Lys  Ser Ile Thr 
    1325                 1330                 1335             


Gly Ile  Tyr Glu Lys Arg Ile  Arg Ile Lys 
    1340                 1345             


<210>  905
<211>  1348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  905

Met Glu Lys Asn Tyr Tyr Leu Gly Leu Asp Leu Gly Ser Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Ala Thr Asp Glu Asp Tyr Asn Phe Leu Arg Leu Lys Gly 
            20                  25                  30          


Lys Thr Ala Trp Gly Ala Arg Ile Phe Ser Glu Ala Lys Asp Cys Lys 
        35                  40                  45              


Glu Arg Arg Thr Tyr Arg Ser Asn Lys Arg Arg Leu Ala Arg Arg Lys 
    50                  55                  60                  


Tyr Arg Leu Val Leu Leu Lys Gln Leu Phe Ala Glu Glu Met Thr Lys 
65                  70                  75                  80  


Val Asp Lys Thr Phe Phe Leu Arg Leu Ala Asn Ser Thr Tyr Trp Glu 
                85                  90                  95      


Glu Asp Lys Thr Lys Asp Ser Asp Gly Asn Glu Ile Gly Lys Asn Leu 
            100                 105                 110         


Ile Phe Lys Thr His Glu Glu Glu Lys Gln Tyr Tyr Thr Lys Tyr Pro 
        115                 120                 125             


Thr Ile Trp His Leu Arg Lys Ala Leu Ile Glu Asn Asp Glu Glu Ala 
    130                 135                 140                 


Leu Ser Asn Leu Arg Tyr Val Tyr Leu Ala Ile His His Ile Val Lys 
145                 150                 155                 160 


Tyr Arg Gly Asn Phe Leu Thr Glu Gly Glu Met Gly Asn Asp Ile Pro 
                165                 170                 175     


Pro Ile Thr Thr Ile Glu Glu Ile Asn Gln Thr Leu Ser Leu Leu Glu 
            180                 185                 190         


Val Asp Ser Ser Glu Asp Ala Thr Asp Lys Thr Thr Tyr Ile Lys Pro 
        195                 200                 205             


Glu Ser Tyr Asn Glu Leu Phe Thr Ile Leu Glu Asn Asn Asn Leu Asn 
    210                 215                 220                 


Lys Val Lys Lys Gln Gln Glu Ile Asp Lys Leu Phe Ile Ser Ser Lys 
225                 230                 235                 240 


Glu Arg Ser Glu Asn Ile Lys Asn Tyr Ile Asn Leu Phe Lys Thr Ile 
                245                 250                 255     


Ile Val Gly Gly Ser Phe Ser Leu Lys Lys Ile Asn Glu Asp Thr Glu 
            260                 265                 270         


Asp Ile Ser Val Asp Phe Thr Lys Asp Phe Glu Asn Ser Glu Ala Asp 
        275                 280                 285             


Ile Ser Lys Ala Leu Gly Asp Ser Phe Asn Leu Val Leu Ile Ala Lys 
    290                 295                 300                 


Lys Tyr Tyr Asp Tyr Ile Ile Leu Lys Glu Leu Leu Gly Lys Asn Lys 
305                 310                 315                 320 


Glu Ile Ser Glu Ala Met Val Gln Ile Tyr Glu Glu His Lys Asp Asp 
                325                 330                 335     


Leu Lys Ser Leu Lys Thr Ile Leu Lys Asp Leu Asp Asn Lys Leu Gly 
            340                 345                 350         


Asn Asp Gly Lys Asp Arg Ile Tyr Tyr Ser Val Phe Lys Gln Glu Pro 
        355                 360                 365             


Thr Glu Lys Phe Lys Glu Thr Asn Tyr Leu Ser Phe Val Gln Gly Gly 
    370                 375                 380                 


Lys Leu Glu Glu Leu Asn Lys Tyr Ile Lys Lys Ile Leu Glu Asn Asn 
385                 390                 395                 400 


Glu Lys Asp Ile Asp Asp Lys Glu Thr Tyr Asn Ala Leu Tyr Glu Lys 
                405                 410                 415     


Ala Cys Lys Gly Asp Leu Phe Lys Lys Gln Ala Asn Ala Cys Thr Ser 
            420                 425                 430         


Leu Ile Pro His Gln Phe His Leu Arg Glu Leu Lys Lys Ile Leu Glu 
        435                 440                 445             


Asn Ala Gly Arg Lys Tyr Ser Phe Leu Asn Asp Ile Ser Asp Lys Ile 
    450                 455                 460                 


Ile Lys Ile Phe Lys Phe Arg Val Pro Tyr Tyr Tyr Gly Pro Leu Asn 
465                 470                 475                 480 


Asn Glu Ser Asn Ala His Ser Trp Val Val Lys Lys Glu Gly Gln Glu 
                485                 490                 495     


Lys Ala Lys Ile Thr Pro Trp Asn Ile Asn Glu Ile Ile Asp Thr Pro 
            500                 505                 510         


Lys Thr Arg Asp Asn Phe Phe Lys Lys Leu Thr Asn Ser Cys Thr Tyr 
        515                 520                 525             


Leu Val Gly Glu Thr Val Leu Pro Lys Thr Ser Leu Tyr Tyr Glu Lys 
    530                 535                 540                 


Tyr Leu Val Leu Asp Arg Leu Asn Lys Met Gln Val Asn Gly Asp Tyr 
545                 550                 555                 560 


Leu Ser Ala Ser Glu Lys Asn Asp Val Leu Lys Phe Ile Leu Ser Arg 
                565                 570                 575     


Lys Thr Thr Thr Ile Glu Gln Ile Arg Thr Tyr Leu Ser Lys Asn Lys 
            580                 585                 590         


Ser Thr Lys Ala Asn Asp Ile Ser Ile Ser Asn Ile Lys Ala Thr Asp 
        595                 600                 605             


Pro Phe Asn Ala Val Ser His Ala Phe Tyr Gly Lys His Pro Asp Leu 
    610                 615                 620                 


Asn Ile Asn Leu Cys Glu Glu Leu Ile Lys Phe Ala Thr Ile Tyr Ala 
625                 630                 635                 640 


Asp Asp Lys Lys Glu Leu Lys Lys Phe Val Ser Ser Asn Phe Asn Leu 
                645                 650                 655     


Thr Asp Glu Phe Ile Lys Glu Tyr Leu Asn Thr Pro Thr Asn Lys Trp 
            660                 665                 670         


Gly Arg Leu Ser Arg Lys Phe Leu Cys Gly Gly Asp Gln Leu Glu Met 
        675                 680                 685             


Tyr Tyr Val Asp Asp Asn Gly Ile Leu His Ser Ile Leu Asn Ile Leu 
    690                 695                 700                 


Glu Glu Thr Asn Glu Asn Leu Gln Glu Ile Leu Asn Asn Glu Lys Tyr 
705                 710                 715                 720 


Gly Phe Asn Gln Lys Ile Ala Asp Phe Asn Asn Thr Leu Thr Ala Asp 
                725                 730                 735     


Lys Thr Thr Glu Glu Gln Ile Glu Asp Ile Leu Asp Ser Thr Pro Ala 
            740                 745                 750         


Leu Met Arg Arg Ser Ile Ile Gln Thr Leu Ala Val Val Asp Asp Val 
        755                 760                 765             


Ile Lys Ala Ala Gly Lys Ala Pro Gln Lys Ile Met Met Glu Val Thr 
    770                 775                 780                 


Arg Thr Asp Gly Lys Lys Glu Glu Thr Lys Ser Arg Tyr Val Gln Leu 
785                 790                 795                 800 


Lys Lys Gln Ile Thr Asn Asp Lys Val Leu Thr Ser Asp Glu Lys Lys 
                805                 810                 815     


Glu Leu Leu Thr Asn Leu Glu Asn Lys Lys Ile Gln Leu Lys Gly Lys 
            820                 825                 830         


His Ile Tyr Leu Tyr Phe Met Gln Met Gly Ile Asp Phe Tyr Thr Gly 
        835                 840                 845             


Lys Ile Met Asp Ile Asn Asp Val Ile Asn Gly Lys Tyr Asp Ile Asp 
    850                 855                 860                 


His Ile Ile Pro Gln Ser Met Ile Lys Asp Asp Ser Leu Asp Asn Met 
865                 870                 875                 880 


Val Leu Val Ser Lys Asp Tyr Asn Gln Lys Val Lys Lys Asp Ile Tyr 
                885                 890                 895     


Pro Ile Cys Asp Glu Ile Arg Asn Asn Met Leu Asp Lys Trp Lys Gly 
            900                 905                 910         


Tyr Tyr Ala Arg Lys Ile Ile Ser Glu Glu Lys Tyr Asn Arg Leu Lys 
        915                 920                 925             


Arg Thr Lys Glu Leu Asn Ala Asp Glu Ile Asn Ala Phe Ile Asn Arg 
    930                 935                 940                 


Gln Ile Asn Val Val Asn Tyr Ser Asn Ile Gln Ile Lys Arg Ile Phe 
945                 950                 955                 960 


Glu Ile Lys Tyr Pro Glu Thr Lys Ile Ile Phe Ser Lys Ala Ser Phe 
                965                 970                 975     


Pro Ser Leu Leu Arg Lys Glu Tyr Glu Ile Ala Lys Met Arg Asp Leu 
            980                 985                 990         


Asn Asp Ala His His Ala Val Asp  Ala Tyr Leu Asn Ile  Val Cys Gly 
        995                 1000                 1005             


Asn Ile  Leu Thr Thr Leu Tyr  Thr Asp Ile Phe Tyr  Met Phe Lys 
    1010                 1015                 1020             


Glu Lys  Lys Glu Asp His Lys  Asn Ile Ser Phe Asn  Met Glu Lys 
    1025                 1030                 1035             


Arg Leu  Ile Asn Tyr Ile Asp  Lys Asn Asn Leu Lys  Asp Lys Ile 
    1040                 1045                 1050             


Lys Thr  Ser Cys Leu Arg His  Asp Ala Leu Ile Thr  Tyr Lys Pro 
    1055                 1060                 1065             


Asp Tyr  Asp Ser Gly Lys Phe  Tyr Lys Glu Thr Ile  Phe Lys Lys 
    1070                 1075                 1080             


Ser Gly  Asp Lys Glu Thr Gly  Leu Ile Pro Leu His  Thr Lys Gly 
    1085                 1090                 1095             


Pro Met  Ser Asp Thr Lys Lys  Tyr Gly Gly Tyr Ser  Gln Leu Ser 
    1100                 1105                 1110             


Gln Ser  Tyr Ile Ile Ala Val  Glu Tyr Lys Leu Lys  Asn Lys Thr 
    1115                 1120                 1125             


Val Lys  Lys Leu Cys Arg Val  Pro Thr Leu Tyr Leu  Lys Gln Tyr 
    1130                 1135                 1140             


Lys Asn  Asp Leu Asn Thr Ile  Ala Lys Ile Met Ile  Asp Asp Glu 
    1145                 1150                 1155             


Lys Ala  Thr Asp Ile Lys Val  Leu Arg Lys Ile Tyr  Leu Asn Gln 
    1160                 1165                 1170             


Lys Ile  Arg Tyr Asn Gly Cys  Tyr Tyr Leu Ile Tyr  Thr Ser Lys 
    1175                 1180                 1185             


Glu Lys  Gln Asn Lys Tyr Lys  Met Ala Tyr Gln Asn  Tyr Leu Asp 
    1190                 1195                 1200             


Asn Asp  Tyr Leu Ile Tyr Leu  Asn Lys Cys Leu Lys  Lys Ile Asp 
    1205                 1210                 1215             


Glu Leu  Asp Asp Gly Glu Leu  Thr Ser Gln Glu Phe  Ile Val Asn 
    1220                 1225                 1230             


Lys Asp  Gly Asp Lys Phe Leu  Ile Asp Lys Asn His  Asn Leu Glu 
    1235                 1240                 1245             


Ile Phe  Lys Ser Ile Lys Glu  Gln Tyr Asn Lys Thr  Ile Tyr Asp 
    1250                 1255                 1260             


Tyr Thr  Pro Tyr Ile Val Gln  Ser Arg Glu Met Lys  Glu Glu Asp 
    1265                 1270                 1275             


Phe Asn  Asn Leu Ser Leu Lys  Lys Gln Ile Asp Thr  Leu Leu Asn 
    1280                 1285                 1290             


Met Ile  Lys Ile Leu Ser Arg  Ser Asn Glu Gln Ala  Lys Phe Glu 
    1295                 1300                 1305             


Thr Ala  Phe Phe Glu Asn Phe  Thr Lys Val Phe Leu  Lys Thr Met 
    1310                 1315                 1320             


Thr Ile  Thr Asn Glu Lys Val  Tyr Ile Val Asn Glu  Ser Pro Ser 
    1325                 1330                 1335             


Gly Leu  Phe Lys Gly Lys Glu  Glu Leu Ile 
    1340                 1345             


<210>  906
<211>  1348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  906

Met Lys Lys Phe Glu Asp Tyr Tyr Leu Gly Leu Asp Leu Gly Thr Ser 
1               5                   10                  15      


Ser Ile Gly Trp Ala Val Thr Asn Glu Lys Tyr Asp Ile Leu Arg Phe 
            20                  25                  30          


Asn Lys Lys Phe Met Trp Gly Thr His Leu Phe Asp Met Gly Asn Thr 
        35                  40                  45              


Ala Ser Glu Arg Arg Ile Phe Arg Thr Ser Arg Arg Arg Leu Lys Arg 
    50                  55                  60                  


Lys Lys Asn Arg Ile Glu Leu Leu Gln Thr Leu Phe Ser Ala Glu Met 
65                  70                  75                  80  


Ser Lys Lys Asp Phe Gly Phe Phe Gln Arg Leu Lys Glu Ser Gln Tyr 
                85                  90                  95      


Phe Ile Glu Asp Lys Thr Phe Lys Asn Lys Asn Thr Leu Phe Met Asp 
            100                 105                 110         


Glu Asn Tyr Thr Asp Gln Asp Tyr His Met Glu Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Lys Ala Leu Ile Asp Asn Glu Glu Ser Ala Phe Glu Ile 
    130                 135                 140                 


Arg Lys Leu Tyr Leu Ala Ile Ser His Ile Leu Ser His Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Glu Gly Glu Leu Ser Asp Asn Leu Asp Phe Asp Asp Ile 
                165                 170                 175     


Phe Asn Glu Phe Val Lys Tyr Leu Asn Glu Glu Leu Asn Ile Asp Phe 
            180                 185                 190         


Lys Ile Lys Ser Asp Val Ser Leu Gln Ala Ile Lys Glu Ile Leu Thr 
        195                 200                 205             


Asp Lys Met Leu Asn Arg Thr Lys Lys Lys Glu Lys Leu Ser Glu Leu 
    210                 215                 220                 


Leu Glu Glu Glu Lys Asn Lys Ile Tyr Gln Glu Ile Trp Lys Leu Leu 
225                 230                 235                 240 


Val Gly Asn Glu Ala Lys Met Ser Thr Ile Phe Asp Asp Lys Glu Tyr 
                245                 250                 255     


Glu Glu Ala Lys Ile Ala Phe Ser Lys Gly Asn Tyr Asp Glu Lys Glu 
            260                 265                 270         


Glu Glu Tyr Glu Ser Ile Leu Gly Glu Lys Ile Leu Phe Val Gln Lys 
        275                 280                 285             


Ala Lys Ala Ile Tyr Asp Trp Met Leu Leu Gln Asn Ile Leu Lys Glu 
    290                 295                 300                 


Asp Lys Tyr Leu Ser Tyr Ser Lys Val Lys Leu Tyr Asn Glu His Lys 
305                 310                 315                 320 


Lys Asp Leu Lys Ile Leu Lys Asp Val Leu Lys Asn Leu Asp Lys Lys 
                325                 330                 335     


Ile Tyr Lys Glu Ile Phe Ser Glu Asn Gly Leu Tyr Glu Glu Tyr Leu 
            340                 345                 350         


Arg Ile Glu Lys Lys Lys Ile Gly Ser Lys Glu Glu Lys Thr Lys Asp 
        355                 360                 365             


Ile Leu Tyr Asn Arg Leu Lys Lys Ile Phe Glu Asn Arg Asp Asn Met 
    370                 375                 380                 


Ala Ile Glu Asp Ile Leu Asn Lys Ile Glu Leu Asp Ser Phe Leu Pro 
385                 390                 395                 400 


Lys Gln Lys Ile Lys Asp Asn Gly Val Ile Pro Arg Gln Val His Gln 
                405                 410                 415     


Lys Glu Leu Glu Ile Ile Leu Lys Asn Ala Glu Lys Tyr Phe Lys Trp 
            420                 425                 430         


Leu Ile Glu Lys Asp Glu Thr Gly Leu Ser Val Ser Glu Lys Ile Met 
        435                 440                 445             


Lys Ile Phe Lys Phe Lys Ile Pro Tyr Tyr Val Gly Pro Leu Asn Ser 
    450                 455                 460                 


Lys Ser Pro Phe Ala Trp Ile Glu Arg Lys Glu Tyr Gly Lys Ile Leu 
465                 470                 475                 480 


Pro Trp Asn Phe Glu Asn Met Val Asp Met Ser Lys Thr Ala Glu Lys 
                485                 490                 495     


Phe Ile Lys Arg Met Thr Asn Lys Cys Thr Tyr Leu Pro Glu Lys Asp 
            500                 505                 510         


Val Ile Pro Lys Asn Ser Leu Leu Tyr Thr Lys Tyr Met Val Leu Asn 
        515                 520                 525             


Glu Leu Asn Asn Leu Lys Ile Arg Gly Glu Lys Ile Lys Val Glu Leu 
    530                 535                 540                 


Lys Gln Lys Ile Tyr Thr Glu Leu Phe Gln Lys Asn Lys Lys Ile Gly 
545                 550                 555                 560 


Met Lys Thr Leu Ile Glu Phe Leu Lys Ser Gln Asn Ile Val Val Thr 
                565                 570                 575     


Lys Val Glu Ile Thr Gly Ile Asp Gly Thr Phe Asn Thr Ser Leu Gly 
            580                 585                 590         


Ser Tyr Ile Asp Met Lys Lys Ile Phe Gly Glu Glu Lys Ile Val Lys 
        595                 600                 605             


Asp Ser Tyr Lys Lys Met Ile Glu Asp Ile Ile Leu Trp Ile Thr Leu 
    610                 615                 620                 


Tyr Gly Glu Glu Lys Lys Leu Ile Lys Ser Lys Ile Glu Glu Lys Tyr 
625                 630                 635                 640 


Pro Glu Lys Val Thr Pro Glu Glu Met Lys Leu Ile Leu Lys Leu Lys 
                645                 650                 655     


Tyr Arg Asp Trp Ser Arg Leu Ser Lys Glu Phe Leu Thr Glu Ile Glu 
            660                 665                 670         


Thr Met Asp Glu Glu Thr Gly Glu Leu Ile Ser Ile Ile Glu Met Leu 
        675                 680                 685             


Trp Arg Lys Asn Glu Asn Ile Met Glu Leu Leu Ser Tyr Lys Tyr Asp 
    690                 695                 700                 


Phe Ser Asn Arg Ile Lys Glu Tyr Asn Gly Ile Asn Glu Lys Glu Lys 
705                 710                 715                 720 


Asn Ile Thr Tyr Glu Asp Leu Val Lys Asp Leu Gln Ile Ser Pro Ser 
                725                 730                 735     


Val Lys Lys Met Ile Trp Arg Ala Leu Val Ile Val Lys Glu Ile Lys 
            740                 745                 750         


Lys Val Thr Asn Lys Ser Pro Lys Lys Val Phe Ile Glu Met Ala Arg 
        755                 760                 765             


Asp Thr Gln Thr Glu Lys Lys Arg Thr Glu Lys Arg Lys Asp Lys Leu 
    770                 775                 780                 


Leu Glu Leu Tyr Lys Asn Ile Lys Asn Asp Asp Ser Ile Lys Glu Leu 
785                 790                 795                 800 


Glu Lys Asn Leu Lys Asp Lys Thr Asn Asp Asp Leu Lys Ser Lys Lys 
                805                 810                 815     


Leu Tyr Leu Tyr Tyr Leu Gln Leu Gly Arg Cys Met Tyr Ser Gly Glu 
            820                 825                 830         


Val Ile Arg Leu Glu Asp Leu Met Asn Asp Asn Leu Tyr Asp Ile Asp 
        835                 840                 845             


His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Phe Asp Asn Thr 
    850                 855                 860                 


Val Leu Val Lys Lys Gln Ile Asn Ala Asp Lys Thr Asn Glu Tyr Pro 
865                 870                 875                 880 


Ile Asp Lys Ser Ile Gln Asn Lys Cys Lys Ala Phe Trp Thr Ser Leu 
                885                 890                 895     


Lys Glu Arg Gly Leu Met Ser Asn Thr Lys Tyr Glu Arg Leu Thr Arg 
            900                 905                 910         


Thr Glu Gly Phe Ser Asp Asp Glu Leu Ala Gly Phe Ile Ser Arg Gln 
        915                 920                 925             


Ile Val Glu Thr Arg Gln Ala Thr Lys Val Leu Ala Asp Leu Leu Lys 
    930                 935                 940                 


Val Ile Phe Gly Ser Asp Thr Arg Ile Val Tyr Val Lys Ala Gly Leu 
945                 950                 955                 960 


Thr Ser Asp Phe Arg Gln Lys Phe Gly Ile Tyr Lys Phe Arg Glu Leu 
                965                 970                 975     


Asn Asp Tyr His His Ala His Asp Gly Tyr Leu Asn Ile Val Thr Gly 
            980                 985                 990         


Asn Ile Tyr Asn Thr Leu Phe Thr  Asp Asn Pro Tyr Asn  Phe Ile Lys 
        995                 1000                 1005             


Asp Arg  Lys Lys Asp Asn Arg  Thr Tyr Asn Leu Asn  Lys Val Phe 
    1010                 1015                 1020             


Glu Arg  Glu Asn Asp Val Trp  Asn Pro Asp Val Met  Leu Ser Lys 
    1025                 1030                 1035             


Ile Glu  Arg Tyr Ile Tyr Lys  Ile Arg Pro Leu Phe  Thr Arg Ala 
    1040                 1045                 1050             


Ser Tyr  Glu Gln Lys Gly Gly  Phe Ser Asp Gln Asn  Ile Ser Ser 
    1055                 1060                 1065             


Glu Lys  Asp Lys Leu Arg Pro  Leu Lys Ser Ser Gly  Val Leu Ser 
    1070                 1075                 1080             


Asn Ile  Lys Tyr Gly Gly Tyr  Asp Lys Ile Lys Asn  Ser Tyr Phe 
    1085                 1090                 1095             


Phe Ile  Val Glu Tyr Thr Glu  Lys Lys Lys Arg Val  Arg Ser Ile 
    1100                 1105                 1110             


Glu Thr  Leu Pro Ile Tyr Ile  Lys Lys Ser Ile Ser  Thr Lys Glu 
    1115                 1120                 1125             


Asp Leu  Glu Lys Tyr Cys Lys  Glu Glu Leu Lys Leu  Gln Glu Pro 
    1130                 1135                 1140             


Lys Val  Leu Val Ser Lys Leu  Asn Tyr Lys Ser Leu  Ile Tyr Ser 
    1145                 1150                 1155             


Asn Gly  His Glu Tyr Tyr Ile  Asn Ser Lys Thr Ala  Asp Ser Ile 
    1160                 1165                 1170             


Thr Ile  Gln Asn Gly Ile Gln  Ile Ile Leu Thr Lys  Glu Asn Tyr 
    1175                 1180                 1185             


Glu Thr  Tyr Arg Lys Ile Tyr  Lys Asn Leu Glu Tyr  Leu Lys Leu 
    1190                 1195                 1200             


Ile Tyr  Lys Asp Glu Lys Ile  Arg Glu Glu Lys Ile  Leu Glu Lys 
    1205                 1210                 1215             


Ile Pro  Lys Ile Leu Lys Asn  Asn Lys Asn Asp Arg  Asp Leu Ser 
    1220                 1225                 1230             


Glu Asp  Glu Ile Ser Glu Lys  Phe Trp Lys Leu Tyr  Lys Glu Ile 
    1235                 1240                 1245             


Phe Glu  Lys Ile Lys Ile Gly  Ile Phe Ser Lys Phe  Lys Ser Thr 
    1250                 1255                 1260             


Leu Pro  Ile Tyr Ile Glu Asp  Cys Lys Lys Leu Lys  Asp Phe Thr 
    1265                 1270                 1275             


Thr Leu  Asn Asn Tyr Ala Lys  Ser Arg Phe Ile Gln  Glu Ile Leu 
    1280                 1285                 1290             


Lys Cys  Leu Asn Ser Gln Gln  Gly Glu Phe Asp Thr  Lys Tyr Thr 
    1295                 1300                 1305             


His Asn  Tyr Ser Lys Lys Tyr  Asn Thr Lys Leu Ser  Lys Ser Lys 
    1310                 1315                 1320             


Ile Leu  Glu Asn Asp Phe Tyr  Ile Ile Asn Gln Ser  Pro Thr Gly 
    1325                 1330                 1335             


Leu Phe  Glu Lys Lys Val Phe  Leu Asp Lys 
    1340                 1345             


<210>  907
<211>  1348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  907

Met Gly Glu Asp Gly Lys Pro Leu Arg Val Lys Gly Lys Thr Ala Ile 
1               5                   10                  15      


Gly Ala Arg Leu Phe Gln Glu Gly Asn Pro Ala Ala Asp Arg Arg Met 
            20                  25                  30          


Phe Arg Thr Thr Arg Arg Arg Leu Asn Arg Arg Lys Trp Arg Leu Lys 
        35                  40                  45              


Leu Leu Asp Glu Ile Phe Asp Pro Tyr Ile Thr Pro Val Asp Ser Thr 
    50                  55                  60                  


Phe Phe Ala Arg Leu Lys Gln Ser Asn Leu Ser Pro Lys Asp Ser Arg 
65                  70                  75                  80  


Lys Glu Phe Lys Gly Ser Met Leu Phe Pro Asp Leu Thr Asp Lys Gln 
                85                  90                  95      


Tyr His Leu Asp Tyr Pro Thr Ile Tyr His Leu Arg His Ala Leu Met 
            100                 105                 110         


Thr Gln Asp Lys Gln Phe Asp Ile Arg Met Val Tyr Leu Ala Ile His 
        115                 120                 125             


His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn Ser Thr Pro Val Asp 
    130                 135                 140                 


Ser Phe Lys Ala Ser Lys Val Asn Phe Asp Asp Gln Phe Lys Lys Leu 
145                 150                 155                 160 


Asn Glu Leu Tyr Thr Ala Ile Asn Pro Glu Glu Ser Phe Gln Ile Asn 
                165                 170                 175     


Leu Ala Asn Ser Glu Lys Ile Gly Gln Gln Phe Leu Asp Pro Ser Ile 
            180                 185                 190         


Arg Lys Phe Asp Lys Lys Lys Gln Ile Pro Lys Ile Val Pro Val Thr 
        195                 200                 205             


Met Asp Asp Lys Ala Val Asp Lys Leu Asn Gly Lys Ile Ala Ser Glu 
    210                 215                 220                 


Ile Ile Asn Ala Ile Leu Gly Tyr Lys Ser Lys Leu Asp Val Ala Val 
225                 230                 235                 240 


Gln Cys Asp Pro Glu Asp Ser Asn Pro Trp Ala Val Lys Phe Asp Asp 
                245                 250                 255     


Glu Asp Ile Asp Ala Lys Leu Gln Lys Ile Leu Pro Glu Met Asp Glu 
            260                 265                 270         


Asn Gln Gln Ser Ile Ile Gly Ile Ile Gln Asp Leu Tyr Ser Gln Val 
        275                 280                 285             


Thr Leu Asn Gln Ile Val Pro Asn Gly Met Ser Leu Ser Glu Ser Met 
    290                 295                 300                 


Ile Lys Lys Tyr Asn Asp His His Asp His Leu Lys Leu Tyr Lys Lys 
305                 310                 315                 320 


Leu Ile Gly Gln Ile Ser Asp Ser Lys Lys Lys Thr Ala Leu Lys Lys 
                325                 330                 335     


Ala Tyr Asp Gln Tyr Val Gly Asn Asp Gly Lys Val Ile Glu Gln Asp 
            340                 345                 350         


Asp Phe Tyr Ser Ser Val Lys Lys Asn Leu Asp Asp Ser Glu Leu Ser 
        355                 360                 365             


Lys Gln Ile Met Asp Leu Ile Asp Ala Asp Lys Phe Met Pro Lys Gln 
    370                 375                 380                 


Arg Thr Ser Gln Asn Gly Val Ile Pro His Gln Leu His Gln Arg Glu 
385                 390                 395                 400 


Leu Asp Glu Ile Ile Glu His Gln Ser Lys Tyr Tyr Pro Trp Leu Ala 
                405                 410                 415     


Glu Ser Asn Pro Asn Lys His Asp Leu His Leu Ala Lys Tyr Lys Ile 
            420                 425                 430         


Glu Glu Leu Val Ala Phe Arg Ile Pro Tyr Tyr Val Gly Pro Met Ile 
        435                 440                 445             


Thr Pro Glu Asp Gln Lys Lys Ser Gly Ala Ala Val Phe Ser Trp Met 
    450                 455                 460                 


Lys Arg Lys Glu Pro Gly Gln Ile Thr Pro Trp Asn Phe Asp Glu Lys 
465                 470                 475                 480 


Val Asp Arg Met Glu Ser Ala Asn Lys Phe Ile Lys Arg Met Thr Thr 
                485                 490                 495     


Lys Asp Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Asp Glu Ser Leu 
            500                 505                 510         


Leu Tyr Glu Lys Phe Lys Val Leu Asn Glu Leu Asn Met Val Arg Val 
        515                 520                 525             


Asn Gly Asp Ser Leu Lys Val Ala Asp Lys Gln Ala Ile Tyr Gln Asp 
    530                 535                 540                 


Leu Phe Glu Asn Tyr Lys His Ile Ser Val Lys Lys Leu Gln Asn Tyr 
545                 550                 555                 560 


Ile Lys Gly Lys Thr Gly Leu Pro Asn Asn Pro Glu Ile Ser Gly Leu 
                565                 570                 575     


Ser Asp Pro Asp Gln Phe Asn Asn Ser Leu Gly Thr Tyr Asn Asp Phe 
            580                 585                 590         


Lys Lys Leu Phe Gly Asp Lys Val Asp Asp Pro Asp Leu Gln Asn Asp 
        595                 600                 605             


Phe Glu Lys Ile Val Glu Trp Ser Thr Val Phe Glu Asp Lys Lys Ile 
    610                 615                 620                 


Leu Arg Ala Lys Leu Asn Glu Ile Thr Trp Leu Thr Asp Lys Gln Lys 
625                 630                 635                 640 


Asp Asp Leu Glu Ser Ser Arg Tyr Gln Gly Trp Gly Arg Leu Ser Lys 
                645                 650                 655     


Lys Leu Leu Thr Gly Ile Val Asn Asp Gln Gly Glu Arg Ile Ile Asp 
            660                 665                 670         


Glu Leu Trp Asn Thr Asn Lys Asn Phe Met Gln Ile Gln Ser Asp Asp 
        675                 680                 685             


Asp Phe Ala Lys Arg Ile His Glu Ala Asn Ala Asp Gln Met Lys Ser 
    690                 695                 700                 


Thr Asn Met Glu Asp Val Leu Ala Asp Ala Tyr Thr Ser Pro Gln Asn 
705                 710                 715                 720 


Lys Lys Ala Ile Arg Gln Val Val Lys Val Val Asp Asp Ile Gln Lys 
                725                 730                 735     


Ala Met Gly Gly Val Ala Pro Lys Phe Ile Ser Ile Glu Phe Thr Arg 
            740                 745                 750         


Ser Glu Asp Arg Asn Pro Arg Arg Thr Ile Ser Arg Gln Arg Gln Leu 
        755                 760                 765             


Glu Asn Thr Leu Lys Asp Thr Ala Lys Ser Leu Val Lys Ser Met Asn 
    770                 775                 780                 


Pro Asn Ile Leu Ser Glu Leu Asp Asn Ala Ser Arg Ser Lys Lys Gly 
785                 790                 795                 800 


Leu Thr Asp Arg Leu Tyr Leu Tyr Phe Thr Gln Leu Gly Lys Asp Met 
                805                 810                 815     


Tyr Thr Gly Glu Pro Ile Asn Ile Asp Glu Ile Ser Asn Tyr Asp Ile 
            820                 825                 830         


Asp His Ile Leu Pro Gln Ala Phe Ile Lys Asp Asp Ser Leu Ser Asn 
        835                 840                 845             


Arg Val Leu Val Ser Lys Ala Val Asn Asn Gly Lys Ser Ala Asn Val 
    850                 855                 860                 


Pro Leu Lys Leu Phe Gly Ala Lys Met Gly His Phe Trp Lys Gln Leu 
865                 870                 875                 880 


Ala Glu Ala Gly Leu Ile Asn Lys His Lys Leu Lys Asn Leu Gln Thr 
                885                 890                 895     


Asn Pro Asp Thr Ile Ser Lys Phe Ala Met His Gly Phe Ile Arg Arg 
            900                 905                 910         


Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Val Ala Asn Ile Leu 
        915                 920                 925             


Gly Asp Lys Tyr Arg Asn Asp Asp Thr Lys Ile Ile Glu Ile Thr Ala 
    930                 935                 940                 


Arg Met Asn His Gln Met Arg Asp Glu Phe Gly Phe Ile Lys Asn Arg 
945                 950                 955                 960 


Glu Ile Asn Asp Tyr His His Ala Phe Asp Ala Tyr Leu Thr Ala Phe 
                965                 970                 975     


Leu Gly Arg Tyr Leu Tyr His Arg Tyr Ile Lys Leu Arg Pro Tyr Phe 
            980                 985                 990         


Val Tyr Gly Asp Phe Lys Lys Phe  Arg Glu Asp Lys Val  Thr Met Arg 
        995                 1000                 1005             


Asn Phe  Asn Phe Leu His Asp  Leu Thr Asp Asp Thr  Gln Ala Arg 
    1010                 1015                 1020             


Ile Ala  Asp Thr Glu Thr Gly  Glu Val Ile Trp Asp  Arg Glu Thr 
    1025                 1030                 1035             


Ser Ile  Lys Gln Leu Arg Asp  Val Tyr His Tyr Lys  Phe Met Leu 
    1040                 1045                 1050             


Ile Ser  Gln Glu Val Tyr Thr  Leu Arg Gly Ala Met  Phe Asn Gln 
    1055                 1060                 1065             


Thr Ile  Tyr Pro Ala Ser Gln  Ala Gly Arg Lys Lys  Leu Ile Pro 
    1070                 1075                 1080             


Met Lys  Ala Asp Lys Pro Val  Asp Val Tyr Gly Gly  Tyr Ser Gly 
    1085                 1090                 1095             


Ser Thr  Asp Ala Tyr Met Ala  Ile Val Arg Ile His  Asp Lys Lys 
    1100                 1105                 1110             


Gly Asp  Lys Tyr Lys Val Val  Gly Val Pro Met Arg  Ala Leu Asn 
    1115                 1120                 1125             


Arg Leu  Asn Thr Ala Lys Asn  Val Ser Asp Ala Lys  Tyr Asp Gln 
    1130                 1135                 1140             


Glu Leu  Lys Glu Val Leu Thr  Pro Gln Phe Thr Lys  Ser Lys Lys 
    1145                 1150                 1155             


Asn Arg  Lys Thr Gly Glu Ile  Thr Gln Thr Val Glu  Asp Phe Glu 
    1160                 1165                 1170             


Val Val  Leu Gly Lys Val Met  Tyr Arg Gln Leu Val  Ile Asp Gly 
    1175                 1180                 1185             


Asp Lys  Lys Phe Met Leu Gly  Ser Ser Thr Tyr Gln  Tyr Asn Ala 
    1190                 1195                 1200             


Lys Gln  Leu Val Leu Ser Asp  Glu Ser Ile Lys Thr  Leu Ala Ser 
    1205                 1210                 1215             


Glu Gly  Lys Leu Asn Glu Ala  Gln Glu Ser Ala Ala  Tyr Asp Lys 
    1220                 1225                 1230             


Val Tyr  Asp Glu Ile Leu Asp  Lys Val Asn Lys Tyr  Phe Ala Leu 
    1235                 1240                 1245             


Tyr Asp  Thr Asn Lys Phe Arg  Gln Lys Leu Ser Leu  Gly Lys Asp 
    1250                 1255                 1260             


Lys Phe  Val Gly Leu Pro Asn  His Asn Val Phe Asp  Gly Ser Lys 
    1265                 1270                 1275             


Lys Ile  Ser Ser Gly Lys Arg  Glu Ile Leu Asn Glu  Ile Leu Asn 
    1280                 1285                 1290             


Gly Leu  His Ala Asn Ala Thr  Leu Gly Thr Leu Ser  Asp Ile Gly 
    1295                 1300                 1305             


Phe Ser  Thr Pro Phe Gly Lys  Met Gln Gln Pro Arg  Gly Ile Gly 
    1310                 1315                 1320             


Leu Ser  Ser Asn Thr Ser Ile  Glu Tyr Glu Ser Phe  Thr Gly Leu 
    1325                 1330                 1335             


Phe Lys  Arg Thr Ile Phe Leu  Lys Asp Leu 
    1340                 1345             


<210>  908
<211>  1348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  908

Met Lys Lys Pro Tyr Thr Ile Ala Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Val Val Val Thr Asp Asp Tyr Arg Val Pro Thr Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Glu Arg Lys Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Asp Thr Ala Glu Gly Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Pro Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Phe Leu Lys Glu Ile Phe Thr Glu Glu Met Ala Lys Val Asp Asp Gly 
                85                  90                  95      


Phe Phe Gln Arg Leu Glu Asp Ser Phe Tyr Val Leu Glu Asp Lys Glu 
            100                 105                 110         


Gly Asn Lys His Pro Ile Phe Ala Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             


His Lys Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Val Asp 
    130                 135                 140                 


Asn Pro Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Val Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Thr Leu Ser Ser 
                165                 170                 175     


Lys Asn Asn Asn Leu Gln Lys Ser Phe Asp His Leu Val Asp Thr Tyr 
            180                 185                 190         


Asn Leu Leu Phe Glu Glu Gln Arg Leu Leu Thr Glu Gly Ile Asn Ala 
        195                 200                 205             


Lys Glu Leu Leu Ser Ala Ala Leu Ser Lys Ser Lys Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ser Leu Ile Pro Gly Gln Lys Lys Thr Gly Ile Phe Gly Asn 
225                 230                 235                 240 


Ile Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ala Asn Phe 
                245                 250                 255     


Gly Leu Ser Lys Asp Val Lys Leu Gln Leu Ala Lys Asp Thr Tyr Ala 
            260                 265                 270         


Asp Asp Leu Asp Ser Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Thr Glu Ser Asp Glu Ile Thr Arg Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Val Lys Arg Tyr Arg Glu His His Lys Asp Leu Val Thr Leu Lys 
                325                 330                 335     


Thr Leu Ile Lys Asp Gln Leu Pro Glu Lys Tyr Gln Glu Ile Phe Leu 
            340                 345                 350         


Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Gln Val Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Tyr Leu Lys Pro Ile Leu Ala Arg Leu Asp 
    370                 375                 380                 


Gly Ser Glu Pro Leu Leu Leu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Val Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Lys Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly His Ser Arg Phe Ala Trp 
    450                 455                 460                 


Val Lys Arg Lys Phe Asp Gly Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Ile Val Asp Glu Glu Ala Ser Ala Gln Ile Phe Ile Glu Lys Met Thr 
                485                 490                 495     


Lys Asn Asp Leu Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Ala Thr Glu Gly Met Thr Arg Pro Gln Phe Leu Ser Ala Asp Gln 
    530                 535                 540                 


Lys Gln Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asn Tyr Phe Lys Lys Ile Glu Cys Trp Asp 
                565                 570                 575     


Ser Val Glu Ile Thr Gly Val Glu Asp Ser Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Gln Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Pro Asp Asn Gln Lys Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Lys Lys Met Ile Ser Lys Arg Leu Asp Gln Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Lys Val Val Leu Asn Lys Leu Glu Arg His His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Gly Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Ser Glu Leu Ser Phe 
    690                 695                 700                 


Ile Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Lys Thr Glu Tyr Ser 
705                 710                 715                 720 


Lys Asp Leu Val Gly Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Ser Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Ile Met Gly 
            740                 745                 750         


Tyr Leu Pro Gln Gln Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Gln Gly Ile Lys Asn Ala Arg Gln Arg Met Arg Lys Leu Glu 
    770                 775                 780                 


Glu Thr Ala Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Asp Asn Ser Gln Leu Gln Asn Asp Lys Arg Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Lys Asp Met Tyr Thr Gly Asp Asp Leu Asp Ile Asp Tyr Leu 
            820                 825                 830         


Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asn 
        835                 840                 845             


Asn Ser Ile Asp Asn Lys Val Leu Thr Ser Gln Gly Ala Asn Arg Gly 
    850                 855                 860                 


Lys Leu Asp Asn Val Pro Ser Glu Ala Ile Val Arg Lys Met Lys Gly 
865                 870                 875                 880 


Tyr Trp Gln Ser Leu Leu Arg Ala Gly Ala Ile Ser Lys Gln Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Gln Val Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Gln Leu Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Phe Asp Asp 
    930                 935                 940                 


His Asn Lys Arg Ile Arg Lys Val His Ile Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Glu Phe Gly Leu Tyr Lys Ile Arg Asp 
                965                 970                 975     


Ile Asn His Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Ala Lys Ala Ile Leu Gly Lys Tyr  Pro Gln Leu Ala Pro  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Pro Lys Tyr  Asn Ser Phe Lys Glu  Arg Gln Lys 
    1010                 1015                 1020             


Ala Thr  Gln Lys Thr Leu Phe  Tyr Ser Asn Ile Leu  Lys Phe Phe 
    1025                 1030                 1035             


Lys Asp  Gln Glu Ser Leu His  Val Asn Ser Asp Gly  Glu Glu Ile 
    1040                 1045                 1050             


Trp Asn  Ala Asn Lys His Leu  Pro Ile Ile Lys Asn  Val Leu Ser 
    1055                 1060                 1065             


Ile Pro  Gln Val Asn Ile Val  Lys Lys Thr Glu Val  Gln Thr Gly 
    1070                 1075                 1080             


Gly Phe  Tyr Lys Glu Ser Ile  Leu Ser Lys Gly Asn  Ser Asp Lys 
    1085                 1090                 1095             


Leu Ile  Pro Arg Lys Asn Asn  Trp Asp Thr Arg Lys  Tyr Gly Gly 
    1100                 1105                 1110             


Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val Leu Val  Ile Ala Lys 
    1115                 1120                 1125             


Met Glu  Lys Gly Lys Ala Lys  Val Leu Lys Pro Val  Lys Glu Met 
    1130                 1135                 1140             


Val Gly  Ile Thr Ile Met Glu  Arg Ile Ala Phe Glu  Glu Asn Pro 
    1145                 1150                 1155             


Val Val  Phe Leu Glu Ala Lys  Gly Tyr Arg Glu Ile  Gln Glu His 
    1160                 1165                 1170             


Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn 
    1175                 1180                 1185             


Gly Arg  Arg Arg Leu Leu Ala  Ser Ala Ser Glu Leu  Gln Lys Gly 
    1190                 1195                 1200             


Asn Glu  Leu Phe Leu Pro Val  Asp Tyr Met Thr Phe  Leu Tyr Leu 
    1205                 1210                 1215             


Ala Ala  His Tyr His Glu Leu  Thr Gly Ser Ser Glu  Asp Val Leu 
    1220                 1225                 1230             


Arg Lys  Lys Tyr Phe Val Glu  Arg His Leu His Tyr  Phe Asp Asp 
    1235                 1240                 1245             


Ile Ile  Gln Met Ile Asn Asp  Phe Ala Glu Arg His  Ile Leu Ala 
    1250                 1255                 1260             


Ser Ser  Asn Leu Glu Lys Ile  Asn His Thr Tyr His  Asn Asn Ser 
    1265                 1270                 1275             


Asp Leu  Pro Val Asn Glu Arg  Ala Glu Asn Ile Ile  Asn Val Phe 
    1280                 1285                 1290             


Thr Phe  Val Ala Leu Gly Ala  Pro Ala Ala Phe Lys  Phe Phe Asp 
    1295                 1300                 1305             


Ala Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser Thr Lys  Glu Val Leu 
    1310                 1315                 1320             


Asn Ala  Thr Leu Ile His Gln  Ser Val Thr Gly Leu  Tyr Glu Thr 
    1325                 1330                 1335             


Arg Ile  Asp Leu Ser Gln Leu  Gly Glu Asn 
    1340                 1345             


<210>  909
<211>  1349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  909

Met Lys Gln Lys Phe Tyr Val Gly Leu Asp Met Gly Thr Ala Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Asp Lys Tyr Asn Ile Ile Lys Arg His Gly 
            20                  25                  30          


Lys Ala Leu Trp Gly Val Arg Leu Phe Lys Thr Ala Asn Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Ile Phe Arg Ala Ala Arg Arg Arg Thr Gln Arg Arg Lys 
    50                  55                  60                  


Gln Arg Met Glu Leu Leu Gln Glu Ile Phe Gly Asp Glu Ile Ser Lys 
65                  70                  75                  80  


Thr Asp Ile Gly Phe Tyr Gln Arg Met Lys Glu Ser Lys Tyr Trp Phe 
                85                  90                  95      


Glu Asp Lys Arg Asp Ile Asn Gly Lys Gln Pro Glu Leu Pro Tyr Ala 
            100                 105                 110         


Leu Phe Val Asp Asp Gly Phe Thr Asp Lys Asp Tyr His Glu Gln Phe 
        115                 120                 125             


Pro Thr Ile Phe His Leu Arg Asn Val Leu Val Asn Glu Trp Thr Gln 
    130                 135                 140                 


Lys Tyr Asp Ile Arg Leu Val Tyr Leu Ala Ile His His Ile Ile Lys 
145                 150                 155                 160 


His Arg Gly His Phe Leu Phe Glu Gly Lys Lys Met Ser Glu Val Thr 
                165                 170                 175     


Asp Phe Gly Ser Ala Phe Glu Ala Phe Cys Lys Val Ala Ser Asp Asn 
            180                 185                 190         


Asp Ile Glu Ile Thr Val Asp Glu Glu Lys Leu Lys Asp Ile Glu Asp 
        195                 200                 205             


Leu Leu Lys Ser Arg Gln Lys Ser Lys Thr Ala Lys Lys Gln Glu Leu 
    210                 215                 220                 


Gly Lys Ile Ile Ala Gly Ala Asp Lys Gln Lys Lys Ala Leu Ala Ala 
225                 230                 235                 240 


Leu Val Thr Gly Cys Met Val Lys Leu Ser Glu Ile Phe Ala Asp Lys 
                245                 250                 255     


Ser Leu Asp Asp Gly Glu Arg Asn Lys Ile Ser Phe Ala Asp Ser Asn 
            260                 265                 270         


Tyr Asp Glu Tyr Ile Ala Asn Val Glu Glu Thr Leu Ala Glu Arg Phe 
        275                 280                 285             


Leu Leu Ile Ala Ala Gly Lys Ala Leu Tyr Asp Trp Ala Val Leu Ser 
    290                 295                 300                 


Asp Ile Leu Glu Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Ile 
305                 310                 315                 320 


Tyr Lys Lys His Gly Asp Asp Leu Arg Lys Leu Lys Gly Leu Leu Lys 
                325                 330                 335     


Thr Arg Pro Asp Glu Tyr Lys Leu Ile Phe Gly Leu Pro Glu Lys Gln 
            340                 345                 350         


Ala Asn Tyr Ser Ala Tyr Ile Gly Met Val Lys Lys Asn Gly Lys Lys 
        355                 360                 365             


Val Pro Ile Asp Lys Lys Cys Thr Lys Ala Asp Phe Tyr Ser Phe Leu 
    370                 375                 380                 


Lys Lys Thr Leu Asn Lys Ile Asn Phe Glu Asp Glu Lys Glu Arg Glu 
385                 390                 395                 400 


Ile Ala Glu Ser Ile Ile Asn Glu Ile Glu Asn Glu Thr Phe Leu Pro 
                405                 410                 415     


Lys Gln Val Ile Ser Asp Asn Gly Val Ile Pro Tyr Gln Leu His Glu 
            420                 425                 430         


Ala Glu Leu Asp Arg Ile Leu Lys Asn Ala Ala Leu Tyr Tyr Pro Phe 
        435                 440                 445             


Leu Ser Glu Lys Asp Asp Glu Gly Tyr Thr Asn Met Asp Lys Ile Lys 
    450                 455                 460                 


Ala Ile Phe Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Thr 
465                 470                 475                 480 


Tyr His Ser Asp Lys Gly Gly Asn Ser Trp Ala Val Arg Lys Gln Asp 
                485                 490                 495     


Gly Lys Ile Tyr Pro Trp Asn Phe Glu Ser Lys Ile Asp Ala Gly Glu 
            500                 505                 510         


Ser Ala Arg Lys Phe Ile Gly Arg Met Thr Asn Lys Cys Thr Tyr Leu 
        515                 520                 525             


Ile Gly Lys Asp Val Leu Pro Lys Glu Ser Leu Leu Tyr Ser Lys Phe 
    530                 535                 540                 


Met Val Leu Asn Glu Leu Asn Asn Leu Arg Ile Asp Gly Glu Lys Val 
545                 550                 555                 560 


Ser Val Glu Ile Lys Gln Ala Val Tyr Glu Asn Val Phe Lys Lys Tyr 
                565                 570                 575     


Gln Arg Val Thr Gly Lys Lys Val Lys Asp Phe Leu Ile Arg Ser Gly 
            580                 585                 590         


Ile Ile Gly Arg Glu Gln Glu Leu Ser Gly Phe Asp Gln Asn Phe Lys 
        595                 600                 605             


Ser Ser Leu Lys Ala Tyr His Asp Ile Lys Gln Ile Ala Gly Asp Ile 
    610                 615                 620                 


Asn Leu Ser Gln Asn Gln Lys Glu Asp Ile Ile Lys Glu Ile Thr Val 
625                 630                 635                 640 


Phe Ala Asp Ser Gln Lys Met Leu Lys Lys Arg Leu Lys Ile Lys Tyr 
                645                 650                 655     


Pro Glu Leu Ser Asp Lys Gln Val Thr Lys Leu Ala Ala Lys Lys Tyr 
            660                 665                 670         


Thr Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Glu Glu Val Glu Ser 
        675                 680                 685             


Val Asn Lys Glu Thr Gly Glu Val Leu Asn Ile Ile Ser Ala Leu Trp 
    690                 695                 700                 


Glu Thr Asn Glu Asn Leu Met Gln Leu Leu Ser Asn Lys Tyr Asp Tyr 
705                 710                 715                 720 


Leu Ser Glu Ile Lys Arg Leu Asn Asp Glu Ala Glu Asp Lys Ser Glu 
                725                 730                 735     


Val Val Thr Tyr Asp Asp Val Gln Lys Leu Tyr Val Ser Pro Ala Val 
            740                 745                 750         


Lys Arg Pro Val Trp Gln Thr Leu Lys Ile Val Glu Glu Ile Ser Lys 
        755                 760                 765             


Ile Met Gly Ser Lys Pro Glu Arg Ile Phe Val Glu Met Ala Arg Glu 
    770                 775                 780                 


Pro Gly Gln Lys Gly Asp Val Lys Val Ser Arg Lys Ser Arg Leu Leu 
785                 790                 795                 800 


Glu Lys Tyr Lys Tyr Cys Lys Lys Glu Ala Pro Glu Ile Tyr Glu Asn 
                805                 810                 815     


Leu Val Lys Glu Asp Asp Asn Lys Leu Arg Ser Asp Lys Leu Phe Leu 
            820                 825                 830         


Tyr Tyr Thr Gln Met Gly Arg Cys Met Tyr Ser Asn Glu Val Ile Asp 
        835                 840                 845             


Ile Glu Asp Leu Phe Thr Asn Arg Tyr Asp Ile Asp His Ile Tyr Pro 
    850                 855                 860                 


Gln Ser Lys Val Met Asp Asp Ser Leu Asp Asn Arg Val Leu Val Lys 
865                 870                 875                 880 


Arg Glu Leu Asn Ser Asn Lys Thr Asp Val Phe Pro Val Pro Lys Glu 
                885                 890                 895     


Tyr Met Asp Lys Ala Lys Thr Leu Trp Asp Met Leu Lys Lys Lys Asp 
            900                 905                 910         


Leu Ile Ser Lys Glu Lys Tyr His Arg Leu Thr Arg Arg Asp Gly Phe 
        915                 920                 925             


Ser Glu Asn Glu Leu Ser Gly Phe Ile Ala Arg Gln Leu Val Glu Thr 
    930                 935                 940                 


Arg Gln Ser Thr Lys Ala Val Thr Gln Leu Leu Thr Lys Ala Tyr Pro 
945                 950                 955                 960 


Glu Ser Glu Ile Val Tyr Ala Lys Ala Asn Ala Val Ser Glu Phe Arg 
                965                 970                 975     


His Lys Phe Gly Phe Thr Lys Val Arg Ser Ile Asn Asp Tyr His His 
            980                 985                 990         


Ala Lys Asp Ala Tyr Leu Asn Ile  Val Val Gly Asn Thr  Tyr Tyr Val 
        995                 1000                 1005             


Lys Phe  Thr Lys Asp Ala Ala  Trp Phe Ile Arg Asn  Asn Pro Gly 
    1010                 1015                 1020             


Arg Thr  Tyr Asn Leu Lys Lys  Met Phe Glu Gln Gly  Thr Val Ser 
    1025                 1030                 1035             


Arg Gly  Gly Glu Thr Ala Trp  Val Ser Gly Glu Asp  Gly Thr Ile 
    1040                 1045                 1050             


Phe Thr  Val Lys Arg Val Met  Gly Lys Asn Asn Ile  Leu Phe Thr 
    1055                 1060                 1065             


Arg Lys  Ala Tyr Glu Val Arg  Gly Gly Leu Phe Asp  Gln Gln Ile 
    1070                 1075                 1080             


Met Lys  Lys Gly Lys Gly Gln  Ile Pro Val Lys Asn  Asn Ser Glu 
    1085                 1090                 1095             


Asp Asp  Arg Leu Cys Ala Thr  Glu Lys Tyr Gly Ala  Tyr Asn Lys 
    1100                 1105                 1110             


Ala Ser  Gly Ala Tyr Phe Met  Leu Val Glu Ser Glu  Asp Lys Lys 
    1115                 1120                 1125             


Gly Asn  Lys Ile Arg Thr Leu  Glu Phe Val Pro Val  Tyr Leu Ala 
    1130                 1135                 1140             


Lys Glu  Leu Glu Lys Ser Glu  Asp Ala Ala Ile Arg  Tyr Cys Val 
    1145                 1150                 1155             


Glu Lys  Leu Glu Leu Lys Asn  Pro Asp Val Arg Val  Arg Lys Ile 
    1160                 1165                 1170             


Lys Met  Asp Ser Leu Phe Asn  Val Asp Gly Phe Arg  Met His Leu 
    1175                 1180                 1185             


Ser Gly  Arg Thr Gly Lys Gln  Leu Ile Phe Lys Cys  Ala Glu Gln 
    1190                 1195                 1200             


Leu Val  Leu Asp Glu Glu Ser  Gln Lys Ile Leu Lys  Lys Thr Ala 
    1205                 1210                 1215             


Lys Val  Phe Ala Asp Tyr Lys  Glu Asn Lys Asn Ile  Lys Ile Ser 
    1220                 1225                 1230             


Asp Lys  Ile Glu Leu Thr Asp  Glu Met Leu Ile Lys  Leu Tyr Glu 
    1235                 1240                 1245             


Leu Phe  Tyr Lys Lys Leu Lys  Asp Thr Val Tyr Asn  Ile Arg Leu 
    1250                 1255                 1260             


Ser Thr  Gln Val Lys Thr Leu  Glu Ser Gly Lys Glu  Lys Phe Glu 
    1265                 1270                 1275             


Lys Leu  Ser Leu Leu Asp Lys  Cys Leu Val Ile Glu  Glu Ile Leu 
    1280                 1285                 1290             


His Leu  Phe Gln Cys Gln Ser  Val Ala Ala Asn Leu  Ser Ser Ile 
    1295                 1300                 1305             


Gly Gly  Ala Ala Thr Ala Gly  Ile Leu Val Leu Asn  Asn Glu Ile 
    1310                 1315                 1320             


Ser Arg  Cys Gly Lys Val Glu  Ile Ile Asn Gln Ser  Ile Thr Gly 
    1325                 1330                 1335             


Phe Tyr  Glu Gln Val Ile Asp  Leu Lys Asn Ile 
    1340                 1345                 


<210>  910
<211>  1349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  910

Met Gln Lys Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Cys Lys Phe Gly Gly Lys 
            20                  25                  30          


Arg Met Trp Gly Ile Arg Leu Phe Asp Gly Ala Gln Thr Ala Glu Glu 
        35                  40                  45              


Arg Arg Met Asn Arg Ser Asn Arg Arg Arg Leu Ser Arg Gln Lys Gln 
    50                  55                  60                  


Arg Ile Asp Leu Leu Gln Glu Leu Phe Ala Lys Glu Ile Ala Lys Val 
65                  70                  75                  80  


Asp Pro Thr Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu Tyr Met Glu 
                85                  90                  95      


Asp Lys Ser Thr Gly Thr Lys Phe Pro Leu Phe Ala Asp Pro Gly Tyr 
            100                 105                 110         


Thr Asp Lys Glu Tyr Tyr Lys Glu Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Gln Glu Leu Ile Lys Asn Pro Glu Ser His Asp Ile Arg Leu Val Tyr 
    130                 135                 140                 


Leu Ala Cys His His Ile Leu Lys Asn Arg Gly His Phe Leu Ile Asn 
145                 150                 155                 160 


Gly Ser Phe Ser Asp Leu Lys Asn Leu Ser Phe Val Cys Arg Glu Met 
                165                 170                 175     


Leu Asp Ala Phe Asn Glu Leu Ser Lys Tyr Gln Leu Met Ile Ser Asp 
            180                 185                 190         


Ile Asp Ala Leu Glu Lys Ile Leu Ser Asp Arg Lys Gln Gln Lys Ser 
        195                 200                 205             


Ala Lys Val Lys Glu Leu Val Gln Ile Phe Glu Cys Val Pro Arg Glu 
    210                 215                 220                 


Asp Lys Ser Asp Ala Lys Ile Cys Lys Glu Ile Leu Ser Asn Leu Cys 
225                 230                 235                 240 


Lys Phe Leu Val Gly Ala Lys Gly Asn Ile Thr Arg Leu Phe Asp Thr 
                245                 250                 255     


Asp Phe Asp Glu Met Glu Thr Ser Ser Phe Ser Phe Gly Asp Ser Lys 
            260                 265                 270         


Tyr Glu Asp Ser Leu Leu Pro Ser Leu Glu Glu Asn Tyr Pro Asp Tyr 
        275                 280                 285             


Ala Asn Val Ile Gln Lys Ile Lys Ala Met Tyr Asp Trp Ser Val Leu 
    290                 295                 300                 


Val Glu Ile Met Ala Gly Glu Asn Tyr Leu Ser Phe Ala Lys Val Asn 
305                 310                 315                 320 


Ala Tyr Lys Lys His Lys Glu Asn Leu Ser Ile Leu Lys Ser Leu Met 
                325                 330                 335     


Lys Lys Tyr Cys Thr Pro Asp Val Tyr Gln Arg Phe Phe Tyr Glu Thr 
            340                 345                 350         


Asp Glu Lys Ser Ser Tyr Gly Asn Tyr Ile Gly Ala Ile His Asn Asn 
        355                 360                 365             


Gly His Ser His Thr Met Lys Lys Cys Asp Glu Glu Thr Phe Tyr Lys 
    370                 375                 380                 


Ser Leu Ser Lys Ile Leu Asp Lys Leu Val Val Gln Lys Thr Asp Gln 
385                 390                 395                 400 


Asp Thr Leu Glu Ile Leu Lys Lys Glu Ala Lys Asn Gln Thr Leu Leu 
                405                 410                 415     


Pro Leu Ala Arg Thr Lys Ala Asn Gly Val Ile Pro Asn Gln Ile His 
            420                 425                 430         


Ile Gln Glu Leu Arg Ala Ile Leu Asp Asn Ala Lys Thr Tyr Leu Ser 
        435                 440                 445             


Phe Leu Asn Glu Ala Asp Glu Tyr Gly Thr Ile Ala Glu Lys Ile Ile 
    450                 455                 460                 


Arg Ile Ala Lys Phe Lys Ile Pro Tyr Tyr Ala Gly Pro Leu Ser Thr 
465                 470                 475                 480 


Arg His Lys Glu Gln Gly Ala Asn Thr Trp Ile Val Arg Lys Gln Glu 
                485                 490                 495     


Gly Arg Ile Tyr Pro Trp Asn Phe Glu Glu Met Val Asp Leu Glu Arg 
            500                 505                 510         


Ser Asn Asn Glu Phe Ile Arg Arg Met Thr Asn Lys Cys Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Asn Ser Leu Leu Tyr Ser Lys Phe 
    530                 535                 540                 


Met Val Leu Asn Glu Leu Asn Asn Leu Lys Ile Arg Gly His Lys Ile 
545                 550                 555                 560 


Ser Val Glu Leu Lys Gln Glu Leu Tyr Lys Glu Leu Phe Cys Lys Lys 
                565                 570                 575     


Ala Lys Val Thr Gly Lys Ala Leu Leu Val Tyr Leu Lys Lys Lys Asp 
            580                 585                 590         


Ala Glu Leu Thr Leu Glu Asp Ile Ser Gly Phe Asp Ala Asp Phe Lys 
        595                 600                 605             


Ser Ser Leu Ser Ile Tyr Ile Asp Phe Lys Asn His Ile Leu Arg Glu 
    610                 615                 620                 


Gln Ile Asp Asp Gly Lys Tyr Gln Ser Ile Val Glu Asn Ile Ile Lys 
625                 630                 635                 640 


Trp Lys Thr Ile Phe Asp Val Asp Ser Lys Met Ile Glu Lys Leu Ile 
                645                 650                 655     


Ala Arg Glu Tyr Pro Asn Met Phe Ser Ser Glu Gln Ile Lys Lys Ile 
            660                 665                 670         


Cys Arg Leu Lys Cys Arg Gly Trp Gly Asn Phe Ser Ser Arg Phe Leu 
        675                 680                 685             


Asn Gly Ile Gln Gly Glu Asn Thr Glu Thr Lys Glu Cys Phe Thr Ile 
    690                 695                 700                 


Ile Glu Ala Leu Trp Lys Thr Asn Tyr Asn Leu Met Gln Leu Leu Ser 
705                 710                 715                 720 


Lys Gln Phe Thr Phe Lys Asp Ala Ile Asp Cys Ile Asn Ala Asp Lys 
                725                 730                 735     


Val Gly Lys Ile Asp Lys Val Thr Tyr Glu Asn Thr Val Lys Asp Leu 
            740                 745                 750         


Val Val Ser Pro Ala Asn Lys Arg Ala Ile Trp Gln Thr Val Gln Ile 
        755                 760                 765             


Thr Glu Glu Ile Lys Lys Val Met Lys Cys Glu Pro Glu Lys Ile Phe 
    770                 775                 780                 


Val Glu Met Ala Arg Gly Gly Glu Lys Glu Lys Lys Arg Thr Ile Ser 
785                 790                 795                 800 


Arg Lys Glu Arg Leu Leu Lys Leu Tyr Met Asp Tyr Lys Asp Glu Asn 
                805                 810                 815     


Arg Asp Trp Ile Lys Glu Ile Glu Ser Arg Asp Glu Gln Asp Phe Thr 
            820                 825                 830         


Asn Arg Lys Leu Tyr Leu Tyr Tyr Thr Gln Ile Gly Arg Cys Met Tyr 
        835                 840                 845             


Ser Gly Glu Glu Ile Ser Leu Glu Glu Leu Met Gln Lys Asn Ser Lys 
    850                 855                 860                 


Trp Asp Lys Asp His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser 
865                 870                 875                 880 


Leu Asp Asn Leu Val Leu Ala Arg Lys Asp Ile Asn Asn Asn Lys Ser 
                885                 890                 895     


Asn Lys Leu Leu Pro Thr Gly Ile Gln Asp Lys Met Lys Lys Tyr Trp 
            900                 905                 910         


Lys Met Leu Leu Lys Tyr Gly Phe Ile Ser Lys Lys Lys Tyr Asp Arg 
        915                 920                 925             


Leu Met Arg Thr Glu Asp Phe Ser Asp Glu Glu Leu Ser Gly Phe Ile 
    930                 935                 940                 


Ala Arg Gln Ile Val Glu Thr Arg Gln Ser Ser Lys Ala Val Ala Glu 
945                 950                 955                 960 


Leu Leu Glu His Leu Asn Glu His Thr Glu Ile Val Tyr Val Lys Ala 
                965                 970                 975     


Glu Leu Val Ser Asp Phe Arg His Asp Asn Asn Met Leu Lys Ser Arg 
            980                 985                 990         


Arg Ile Asn Asp Tyr His His Ala  Lys Asp Ala Phe Leu  Asn Ile Val 
        995                 1000                 1005             


Val Gly  Asn Val Tyr Asn Ala  Lys Phe Thr Ala Asn  Pro Arg Asn 
    1010                 1015                 1020             


Trp Ile  Lys Lys Asn Arg Asn  Thr Asn Tyr Ser Ile  Arg Arg Val 
    1025                 1030                 1035             


Phe Asp  Tyr Thr Val Lys Arg  Gly Ser Thr Ile Val  Trp Gln Gly 
    1040                 1045                 1050             


Val Lys  Asp Gly Gly His Ser  Ile Asp Gly Ile Lys  Lys Thr Met 
    1055                 1060                 1065             


Ala Arg  Asn Asp Ile Leu Tyr  Thr Glu Arg Thr Tyr  Cys Glu Gln 
    1070                 1075                 1080             


Gly Glu  Leu Phe Asn Ala Thr  Leu Ile Lys Lys Gly  Asn Pro Asn 
    1085                 1090                 1095             


Ala Ile  Gly Leu Lys Lys Gly  Leu Asp Pro Met Lys  Tyr Gly Gly 
    1100                 1105                 1110             


Tyr Ser  Ser Pro Lys Thr Ser  Ala Phe Ala Phe Ile  Glu Phe Asp 
    1115                 1120                 1125             


Gly Lys  Lys Gly Arg Gln Asn  His Leu Val Glu Ile  Pro Val Tyr 
    1130                 1135                 1140             


Ile Ala  Asn Ile Ala Ala Arg  Gln Pro Glu Tyr Ile  Lys Asn His 
    1145                 1150                 1155             


Leu Glu  Glu Lys Lys Gly Tyr  Gln Asn Val Ile Val  Lys Lys Tyr 
    1160                 1165                 1170             


Pro Ile  Gln Lys Asn Ala Leu  Ile Lys Leu Asp Gly  Tyr Pro Ala 
    1175                 1180                 1185             


Arg Leu  Arg Gly Ala Asn Glu  Lys Ser Ile Thr Leu  Lys Asn Ala 
    1190                 1195                 1200             


Val Gln  Leu Val Leu Lys Pro  Glu Tyr His Glu Thr  Ile Arg Asn 
    1205                 1210                 1215             


Val Glu  Lys Tyr Leu Glu Tyr  Ile Asp Lys Asp Glu  Ser Val Ser 
    1220                 1225                 1230             


Lys Ala  Ala Arg Phe Ser His  Thr Glu Leu Asp Lys  Leu Tyr Asp 
    1235                 1240                 1245             


Glu Leu  Cys Phe Lys Ala Glu  Asn Ser Ile Tyr Lys  Lys Arg Pro 
    1250                 1255                 1260             


Ala Asn  Gln Ser Ala Thr Leu  Lys Asn Gly Gln Lys  Thr Phe Leu 
    1265                 1270                 1275             


Ala Ile  Thr Asn Leu Lys Glu  Lys Ala Gln Val Ile  Asn Asn Ile 
    1280                 1285                 1290             


Ile Thr  Met Phe Arg Cys Asp  Ala Thr Thr Thr Thr  Asn Leu Lys 
    1295                 1300                 1305             


Thr Ile  Gly Gly Ser Ala Asn  Ala Gly Gly Met Gln  Val Asn Lys 
    1310                 1315                 1320             


Asn Thr  Leu Thr Ser Gln Lys  Val Val Leu Val His  Gln Ser Ile 
    1325                 1330                 1335             


Thr Gly  Leu Phe Gly Thr Glu  Glu Glu Leu Leu 
    1340                 1345                 


<210>  911
<211>  1350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  911

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Thr Val Leu Gly Asn Thr Asp Lys Ser His Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Thr Glu Asp Lys Arg 
            100                 105                 110         


Gly Glu Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Lys Tyr 
        115                 120                 125             


His Glu Asn Phe Pro Thr Ile Tyr His Leu Arg Gln Tyr Leu Ala Asp 
    130                 135                 140                 


Asn Pro Glu Lys Val Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Lys Phe Asp Thr 
                165                 170                 175     


Arg Asn Asn Asp Val Gln Arg Leu Phe Gln Glu Phe Leu Ala Val Tyr 
            180                 185                 190         


Asp Asn Thr Phe Glu Asn Ser Ser Leu Gln Glu Gln Asn Val Gln Val 
        195                 200                 205             


Glu Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 


Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Arg Phe Ala Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     


Glu Leu Glu Glu Lys Thr Pro Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu 
        275                 280                 285             


Leu Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Val Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys 
                325                 330                 335     


Gln Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         


Asp Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             


Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu 
    370                 375                 380                 


Gly Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Asp Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp 
    450                 455                 460                 


Leu Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 


Ile Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Asn Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Lys Thr Glu Gln Gly Lys Thr Ala Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr Lys 
545                 550                 555                 560 


Asp Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     


Val Asp Leu Thr Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ala Ser 
            580                 585                 590         


Tyr Gly Thr Tyr His Asp Leu Arg Lys Ile Leu Asp Lys Asp Phe Leu 
        595                 600                 605             


Asp Asn Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Asn Tyr 
625                 630                 635                 640 


Ser Asp Leu Leu Thr Lys Glu Gln Val Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
        675                 680                 685             


Asn Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser 
    690                 695                 700                 


Phe Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn 
705                 710                 715                 720 


Leu Asn Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met 
            740                 745                 750         


Gly His Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu 
    770                 775                 780                 


Thr Asp Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr 
            820                 825                 830         


Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             


Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asp Val Pro Ser Glu Asp Val Val Asn Arg Met Arg 
865                 870                 875                 880 


Pro Phe Trp Asn Lys Leu Leu Ser Ser Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     


Tyr Asn Asn Leu Thr Lys Lys Glu Leu Thr Pro Asp Asp Lys Ala Gly 
            900                 905                 910         


Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val 
        915                 920                 925             


Ala Arg Met Leu Asp Glu Arg Phe Asn Lys Glu Phe Asp Asp Asn Asn 
    930                 935                 940                 


Lys Arg Ile Arg Arg Val Lys Ile Val Thr Leu Lys Ser Asn Leu Val 
945                 950                 955                 960 


Ser Ser Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile Asn 
                965                 970                 975     


Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Val Lys 
            980                 985                 990         


Ala Leu Leu Val Lys Tyr Pro Lys  Leu Glu Pro Glu Phe  Val Tyr Gly 
        995                 1000                 1005             


Glu Tyr  Pro Lys Tyr Asn Ser  Tyr Arg Glu Arg Lys  Thr Ala Thr 
    1010                 1015                 1020             


Gln Lys  Met Phe Phe Tyr Ser  Asn Ile Met Asn Met  Phe Lys Ser 
    1025                 1030                 1035             


Lys Val  Lys Leu Ala Asp Asp  Gln Ile Val Glu Arg  Pro Met Ile 
    1040                 1045                 1050             


Glu Val  Asn Asp Glu Thr Gly  Glu Ile Ala Trp Asp  Lys Thr Lys 
    1055                 1060                 1065             


His Ile  Thr Thr Val Lys Lys  Val Leu Ser Tyr Pro  Gln Val Asn 
    1070                 1075                 1080             


Ile Val  Lys Lys Val Glu Glu  Gln Thr Ile Gly Gln  Asn Gly Gly 
    1085                 1090                 1095             


Leu Phe  Asp Asp Asn Pro Lys  Ser Pro Leu Glu Val  Ile Pro Ser 
    1100                 1105                 1110             


Lys Leu  Val Pro Leu Lys Lys  Ala Leu Asn Pro Glu  Lys Tyr Gly 
    1115                 1120                 1125             


Gly Tyr  Gln Lys Pro Thr Thr  Ala Tyr Pro Ile Leu  Leu Ile Val 
    1130                 1135                 1140             


Asp Thr  Lys Gln Leu Ile Pro  Ile Ser Val Met Asp  Lys Lys Arg 
    1145                 1150                 1155             


Phe Glu  Gln Asn Pro Val Lys  Phe Leu Lys Asp Lys  Gly Tyr Gln 
    1160                 1165                 1170             


Gln Ile  Glu Lys Asn Asn Phe  Val Lys Leu Pro Lys  Tyr Thr Leu 
    1175                 1180                 1185             


Val Asp  Ile Gly Asn Gly Ile  Lys Arg Leu Trp Ala  Ser Ser Lys 
    1190                 1195                 1200             


Glu Val  His Lys Gly Asn Gln  Leu Val Val Ser Lys  Lys Ser Gln 
    1205                 1210                 1215             


Asp Leu  Leu Tyr His Ala His  His Leu Asp Asn Asp  Tyr Ser Asn 
    1220                 1225                 1230             


Glu Tyr  Val Lys Asn His Tyr  Gln Gln Phe Asp Ile  Leu Phe Asn 
    1235                 1240                 1245             


Glu Ile  Thr Ser Phe Ser Lys  Lys Cys Lys Leu Gly  Lys Glu His 
    1250                 1255                 1260             


Ile Gln  Lys Ile Glu Glu Ala  Tyr Ser Lys Glu Arg  Asp Ser Ala 
    1265                 1270                 1275             


Ser Ile  Glu Glu Leu Ala Asp  Gly Phe Ile Lys Leu  Leu Gly Phe 
    1280                 1285                 1290             


Thr Gln  Leu Gly Ala Thr Ser  Pro Phe Ser Phe Leu  Gly Ile Lys 
    1295                 1300                 1305             


Leu Asn  Gln Lys Gln Tyr Thr  Gly Lys Lys Asp Tyr  Leu Leu Pro 
    1310                 1315                 1320             


Cys Met  Glu Ala Thr Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr 
    1325                 1330                 1335             


Glu Thr  Arg Ile Asp Leu Ser  Lys Leu Gly Gly Asp  
    1340                 1345                 1350 


<210>  912
<211>  1350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  912

Met Gly Lys Asn Tyr Tyr Leu Gly Leu Asp Met Gly Thr Ser Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Glu Lys Tyr Arg Ile Leu Arg Ala Lys Gly 
            20                  25                  30          


Lys Asp Leu Trp Gly Val Arg Leu Phe Ser Glu Ala Lys Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Thr His Arg Thr Ser Arg Arg Arg Leu Asp Arg Glu Lys 
    50                  55                  60                  


Ala Arg Ile Ala Cys Leu Lys Glu Met Phe Ala Glu Glu Ile Asn Lys 
65                  70                  75                  80  


Ile Asp Pro Gly Phe Tyr Gln Arg Leu Asn Asp Ser Lys Tyr Phe Leu 
                85                  90                  95      


Glu Asp Lys Lys Glu His Gln Pro Tyr Ala Ile Phe Ala Asp Thr Gly 
            100                 105                 110         


Tyr Thr Asp Lys Glu Tyr Tyr Asp Glu Tyr Pro Thr Ile Phe His Leu 
        115                 120                 125             


Arg Lys Ala Leu Leu Asn Ile Glu Asn Asp Lys Lys Lys Tyr Asp Val 
    130                 135                 140                 


Arg Leu Val Tyr Leu Ala Ile Leu Asn Met Phe Lys His Arg Gly His 
145                 150                 155                 160 


Phe Leu Asn Ala Asn Leu Asp Asp Lys Ser Gly Gly Asn Leu Lys Glu 
                165                 170                 175     


Tyr Ile Glu Met Leu His Gln Ser Met Val Asn Ile Leu Asn Ile Asn 
            180                 185                 190         


Leu Lys Asn Val Glu Ile Glu Lys Ile Glu Lys Ile Leu Lys Ser Thr 
        195                 200                 205             


Gln Tyr Ser Asn Ser Glu Arg Leu Glu Asn Leu Leu Glu Leu Tyr Glu 
    210                 215                 220                 


Leu Ser Lys Asn Lys Asn Lys Ile Glu Thr Glu Ile Phe Lys Phe Ile 
225                 230                 235                 240 


Cys Gly Leu Lys Gly Thr Ile Ser Lys Ile Tyr Val Glu Asp Lys Phe 
                245                 250                 255     


Glu Asp Glu Phe Ala Lys Met Thr Leu Ser Phe Arg Asp Ser Asn Phe 
            260                 265                 270         


Asp Glu Lys Ile Ile Glu Ile Glu Asp Asn Leu Asp Asp Asp Lys Tyr 
        275                 280                 285             


Asp Met Phe Leu Leu Ile Lys Gln Ile His Asp Trp Ser Val Leu Ala 
    290                 295                 300                 


Asn Ile Met Asn Gly Glu Glu Tyr Leu Ser Val Ala Arg Val Lys Leu 
305                 310                 315                 320 


Tyr Asp Lys His Lys Lys Asp Leu Glu Val Leu Lys Lys Tyr Tyr Lys 
                325                 330                 335     


Gln Asn Ser Met Glu Glu Tyr Asn Lys Met Phe Arg Gln Met Asn Asp 
            340                 345                 350         


Gly Asn Tyr Ser Ala Tyr Val Gly Ser Val Ile Tyr Lys Asp Asn Ser 
        355                 360                 365             


Val Arg Arg Gly Cys Lys Ser Lys Lys Glu Asp Phe Tyr Lys Asn Ile 
    370                 375                 380                 


Leu Asn Thr Ile Lys Ser Trp Glu Asp Cys Glu Ala Lys Glu Tyr Ile 
385                 390                 395                 400 


Thr Ser Glu Ile Asp Lys Gly Asn Phe Leu Pro Lys Gln Ile Thr Ala 
                405                 410                 415     


Ser Asn Gly Val Ile Pro Asn Gln Val His Lys Lys Glu Leu Lys Lys 
            420                 425                 430         


Ile Leu Lys Asn Ala Ser Glu Tyr Leu Asn Phe Leu Asn Glu Lys Asp 
        435                 440                 445             


Glu Ser Gly Tyr Thr Ile Ser Glu Lys Ile Val Lys Leu Phe Glu Phe 
    450                 455                 460                 


Gln Ile Pro Tyr Tyr Val Gly Pro Ile Ala Tyr Asn Ile Gly Asn Asp 
465                 470                 475                 480 


Glu Ser Lys His Arg His Asn Met Trp Ser Val Arg Lys Glu Lys Gly 
                485                 490                 495     


Pro Ile Tyr Pro Trp Asn Phe Glu Gln Lys Ile Asp Ile Lys Lys Ser 
            500                 505                 510         


Ser Glu Lys Phe Ile Arg Asn Leu Ile Asn His Cys Thr Tyr Leu Asn 
        515                 520                 525             


Asp Glu Glu Val Leu Pro Lys Asn Ser Leu Leu Tyr Glu Lys Phe Met 
    530                 535                 540                 


Val Leu Asn Glu Leu Asn Lys Leu Lys Ile Asn Gly Glu Lys Ile Ser 
545                 550                 555                 560 


Val Glu Leu Lys Gln Asn Ile Phe Asn Asp Leu Phe Lys Lys Gly Lys 
                565                 570                 575     


Lys Val Thr Lys Lys Gly Leu Ile Lys Tyr Leu Lys Glu Gln Gly Glu 
            580                 585                 590         


Ile Asp Asn Cys Glu Glu Val Glu Ile Ser Gly Ile Asp Gly Asp Phe 
        595                 600                 605             


Thr Asn Lys Leu Ser Asn Tyr Lys Lys Phe Ala Asp Ile Phe Gly Val 
    610                 615                 620                 


Gln Ser Leu Thr Tyr Glu Gln Thr Asp Ile Ala Glu Asn Ile Ile Arg 
625                 630                 635                 640 


Tyr Ser Thr Ile Tyr Gly Asp Ser Arg Lys Phe Leu Glu Glu Arg Ile 
                645                 650                 655     


Arg Glu Glu Tyr Ser Asn Val Leu Asp Glu Lys Gln Ile Lys Arg Ile 
            660                 665                 670         


Leu Gly Met Lys Phe Lys Asp Trp Gly Arg Leu Ser Lys Glu Leu Leu 
        675                 680                 685             


Glu Leu Ser Gly Val Asp Lys Glu Thr Gly Glu Ile Ala Ser Val Ile 
    690                 695                 700                 


Ser Arg Met Trp Asn Asp Asn Tyr Asn Leu Met Glu Leu Ile Ala Thr 
705                 710                 715                 720 


Glu Arg Phe Ser Tyr Ala Tyr Glu Ile Glu Gln Arg Ser Lys Lys Leu 
                725                 730                 735     


Glu Lys Thr Ile Ser Thr Ile Glu Tyr Glu Asp Leu Asp Glu Leu Tyr 
            740                 745                 750         


Met Ser Thr Pro Val Lys Arg Met Val Trp Gln Thr Ile Leu Val Leu 
        755                 760                 765             


Lys Glu Leu Glu Glu Val Leu Gly Cys Pro Pro Lys Arg Ile Phe Val 
    770                 775                 780                 


Glu Met Ala Arg Asp Val Asn Ala Pro Lys Asp Arg Lys Asp Ser Arg 
785                 790                 795                 800 


Lys Lys Lys Leu Leu Glu Leu Tyr Lys Asn Cys Lys Lys Glu Glu Gly 
                805                 810                 815     


Asp Trp Ser Lys Glu Leu Asn Ala Arg Glu Glu Ser Glu Phe Lys Ser 
            820                 825                 830         


Lys Lys Leu Tyr Leu Tyr Tyr Met Gln Lys Gly Arg Cys Met Tyr Ser 
        835                 840                 845             


Gly Lys Pro Ile Asp Leu Lys Asn Leu Leu Asn Asp Asn Leu Tyr Asp 
    850                 855                 860                 


Ile Asp His Ile Tyr Pro Arg His Phe Val Lys Asp Asp Ser Ile Glu 
865                 870                 875                 880 


Asn Asn Leu Val Leu Val Glu Lys Asn Phe Asn Ala His Lys Ser Asp 
                885                 890                 895     


Asn Tyr Pro Ile Glu Asn Glu Ile Arg Gln Lys Met His Ser Met Trp 
            900                 905                 910         


Lys Met Leu Arg Asp Gly Asn Phe Ile Thr Glu Glu Lys Tyr Ser Arg 
        915                 920                 925             


Leu Ile Arg Lys Glu Glu Phe Thr Asp Asp Glu Lys Ala Lys Phe Ile 
    930                 935                 940                 


Ser Arg Gln Ile Val Glu Thr Arg Gln Gly Thr Lys Val Ile Thr Asp 
945                 950                 955                 960 


Leu Phe Glu Gln Thr Phe Pro Glu Ser Asp Ile Val Tyr Val Lys Ala 
                965                 970                 975     


Gly Asn Val Ser Glu Phe Arg His Lys Phe Lys Leu Ile Lys Cys Arg 
            980                 985                 990         


Asn Val Asn Asp Phe His His Ala  Asn Asp Ala Tyr Leu  Asn Ile Val 
        995                 1000                 1005             


Val Gly  Asn Val Tyr Tyr Thr  Lys Phe Thr Lys Ser  Ala Leu Asn 
    1010                 1015                 1020             


Phe Ile  Lys Asp Tyr Lys Lys  Asn Pro Glu Lys Asn  Lys Tyr His 
    1025                 1030                 1035             


Met Asp  His Met Phe Gln Tyr  Pro Val Ser Arg Gly  Gly Val Asp 
    1040                 1045                 1050             


Ala Trp  Ile Thr Lys Gly Ala  Glu Ser Ile Ser Met  Val Arg Lys 
    1055                 1060                 1065             


Met Met  Ala Lys Asn Thr Pro  Met Val Thr Arg Met  Asn Tyr Glu 
    1070                 1075                 1080             


Lys His  Ser Gly Gln Lys Gly  Gly Ile Ser Asp Gln  Asn Ile Cys 
    1085                 1090                 1095             


Ser Ala  Lys Ile Ala Lys Lys  Asn Val Tyr Leu Pro  Ile Lys Met 
    1100                 1105                 1110             


Gly Asp  Glu Lys Leu Lys Asn  Val Glu Lys Tyr Gly  Gly Tyr Gly 
    1115                 1120                 1125             


Ser Leu  Thr Ala Lys Tyr Phe  Phe Leu Val Glu His  Thr Ile Lys 
    1130                 1135                 1140             


Lys Lys  Arg Ile Arg Thr Ile  Glu Ala Tyr Pro Met  Tyr Leu Asp 
    1145                 1150                 1155             


Ala Leu  Asn Lys Glu Asn Lys  Leu Leu Asn Tyr Cys  Val Gln Val 
    1160                 1165                 1170             


Leu Glu  Tyr Asp Glu Pro Lys  Ile Ile Met Glu Lys  Ile Lys Val 
    1175                 1180                 1185             


Lys Ser  Leu Phe Lys Ile Asn  Gly Tyr Tyr Tyr Tyr  Leu Asn Ala 
    1190                 1195                 1200             


Lys Ser  Val Asn Arg Ile Ile  Trp Gly Asn Ala Thr  Gln Leu Phe 
    1205                 1210                 1215             


Leu Glu  Asn Asp Ile Asn Glu  Tyr Val Arg Lys Ile  Ser Lys Asn 
    1220                 1225                 1230             


Ile Glu  Cys Ile Asp Ile Asn  Ser Glu Asn Asn Ile  Lys Val Tyr 
    1235                 1240                 1245             


Asp Ile  Phe Ile Glu Lys Tyr  Ser Asn Lys Leu Phe  Ser Asn Arg 
    1250                 1255                 1260             


Leu Gly  Asn Leu Lys Glu Ala  Ile Ile Gln Asn Arg  Asn Lys Phe 
    1265                 1270                 1275             


Ile Glu  Leu Lys Ile Asn Glu  Gln Ile Asn Val Ile  Leu Glu Leu 
    1280                 1285                 1290             


Val Arg  Tyr Ser Gln Asp Gly  Val Tyr Ser Ile Asn  Leu Ser Gln 
    1295                 1300                 1305             


Leu Gly  Leu Ser Ser Thr Tyr  Ala Ile Asn Arg Thr  Ser Lys Glu 
    1310                 1315                 1320             


Ile Ser  Lys Met Lys Glu Cys  Lys Leu Ile Asn Gln  Ser Val Thr 
    1325                 1330                 1335             


Gly Leu  Tyr Glu Asn Glu Ile  Asp Leu Leu Thr Val  
    1340                 1345                 1350 


<210>  913
<211>  1350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  913

Met Gln Gln Tyr Tyr Leu Gly Val Asp Met Gly Ser Ala Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Thr Asp Glu Lys Tyr Gln Leu Val Arg Lys Lys Gly Lys 
            20                  25                  30          


Asp Leu Trp Gly Val Arg Thr Phe Asp Ile Ala Gln Thr Ala Glu Val 
        35                  40                  45              


Arg Arg Val Ser Arg Thr Asn Arg Arg Arg Gln Asn Arg Arg Lys Gln 
    50                  55                  60                  


Arg Ile Gln Ile Leu Gln Glu Leu Leu Gly Glu Glu Val Leu Lys Ile 
65                  70                  75                  80  


Asp Ala Gly Phe Phe His Arg Met Lys Glu Ser Arg Tyr Val Ala Glu 
                85                  90                  95      


Asp Lys Arg Thr Leu Asp Gly Lys Gln Val Glu Leu Pro Tyr Ala Leu 
            100                 105                 110         


Phe Val Asp Gln Gly Phe Thr Asp Lys Asp Phe Tyr Lys Gln Phe Pro 
        115                 120                 125             


Thr Ile Asn His Leu Ile Val Tyr Leu Met Thr Thr Ser Asp Thr Pro 
    130                 135                 140                 


Asp Ile Arg Leu Val Tyr Leu Ala Leu His Tyr Tyr Met Lys Asn Arg 
145                 150                 155                 160 


Gly Asn Phe Leu His Ser Gly Asp Ile Asn Asp Val Lys Asp Ile Gln 
                165                 170                 175     


Ser Ile Leu Glu Gln Leu Glu Asn Val Leu Lys Glu Tyr Val Asp Asp 
            180                 185                 190         


Trp Glu Leu Ser Leu Lys Asp Lys Val Asp Ala Ile Lys Glu Ile Tyr 
        195                 200                 205             


Asn Lys Asp Leu Gly Arg Gly Glu Arg Lys Lys Ala Phe Ile Asn Thr 
    210                 215                 220                 


Leu Gly Val Lys Thr Lys Ser Ala Lys Ala Phe Cys Ser Leu Ile Ser 
225                 230                 235                 240 


Gly Gly Ser Thr Asn Leu Ala Glu Leu Phe Asp Asp Ser Gly Leu Lys 
                245                 250                 255     


Glu Ser Glu Tyr Ala Lys Ile Glu Phe Ala Asn Ala Asn Phe Glu Asp 
            260                 265                 270         


Ser Val Glu Gly Ile Gln Ala Leu Leu Glu Asp Arg Phe Ala Val Ile 
        275                 280                 285             


Glu Ala Ala Lys Arg Leu Tyr Asp Trp Lys Ile Leu Thr Asp Ile Leu 
    290                 295                 300                 


Gly Asp Asn Ala Ser Leu Ala Glu Ala Arg Val Lys Ser Tyr Glu Thr 
305                 310                 315                 320 


His His Glu Gln Leu Val Glu Leu Lys Ser Phe Ile Lys Lys Tyr Leu 
                325                 330                 335     


Asp Arg Lys Ile Tyr Gln Asp Ile Phe Ile Asn Pro Asn Ile Ala Asn 
            340                 345                 350         


Asn Tyr Pro Ala Tyr Val Gly His Thr Lys Ile Asn Gly Lys Lys Gln 
        355                 360                 365             


Glu Leu Glu Val Lys Arg Ala Lys Arg Asn Asp Phe Tyr Ala Tyr Ile 
    370                 375                 380                 


Lys Lys Gln Val Ile Asp Pro Ile Lys Lys Lys Val Ser Asp Lys Ala 
385                 390                 395                 400 


Val Leu Ala Arg Leu Ala Glu Ile Glu Ser Leu Ile Glu Val Asn Lys 
                405                 410                 415     


Tyr Leu Pro Leu Gln Val Asn Ser Asp Asn Gly Val Ile Pro Tyr Gln 
            420                 425                 430         


Ile Lys Leu Asn Glu Leu Arg Arg Ile Phe Asn Asn Leu Glu Asn Arg 
        435                 440                 445             


Leu Pro Val Leu Lys Glu Asn Arg Asp Lys Ile Ile Lys Thr Phe Ser 
    450                 455                 460                 


Tyr Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Gly Val Asn Arg Asn 
465                 470                 475                 480 


Gly Lys Ser Thr Asn Trp Met Val Arg Lys Glu Gly Glu Glu Gly Lys 
                485                 490                 495     


Ile Tyr Pro Trp Asn Phe Glu Glu Lys Val Asp Leu Glu Ala Ser Ala 
            500                 505                 510         


Glu Lys Phe Ile Arg Arg Met Thr Asn Lys Cys Thr Tyr Leu Val Asn 
        515                 520                 525             


Glu Asp Val Leu Pro Lys Tyr Ser Leu Leu Tyr Ser Lys Tyr Leu Val 
    530                 535                 540                 


Leu Ser Glu Leu Asn Asn Leu Arg Leu Asp Gly Arg Pro Leu Glu Val 
545                 550                 555                 560 


Ser Val Lys Gln Glu Ile Tyr Glu Asn Val Phe Lys Arg Asn Arg Lys 
                565                 570                 575     


Val Thr Leu Lys Lys Ile Lys Asn Tyr Leu Leu Lys Glu Gly Val Ile 
            580                 585                 590         


Ser Glu Lys Asp Glu Leu Ser Gly Leu Ala Asp Asp Val Lys Ser Ser 
        595                 600                 605             


Leu Thr Ala Tyr His Asp Phe Lys Glu Lys Leu Gly His Leu Thr Leu 
    610                 615                 620                 


Thr Glu Asp Gln Met Glu Lys Ile Ile Leu Asn Val Thr Leu Phe Gly 
625                 630                 635                 640 


Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Ala Ala Leu Tyr Pro Asn 
                645                 650                 655     


Ile Asp Glu Lys Ser Leu Ser Arg Met Ala Thr Phe Asn Tyr Arg Asp 
            660                 665                 670         


Trp Gly Arg Leu Ser Lys Lys Phe Leu Ser Glu Ile Thr Ser Val Asp 
        675                 680                 685             


Gln Glu Thr Gly Glu Leu Arg Thr Ile Ile Gln Cys Met Tyr Glu Thr 
    690                 695                 700                 


Gln Asn Asn Leu Met Gln Leu Leu Ser Glu Pro Tyr His Phe Val Glu 
705                 710                 715                 720 


Ala Ile Glu Lys Glu Asn Pro Lys Val Asp Leu Glu Ser Ile Ser Tyr 
                725                 730                 735     


Arg Ile Val Asn Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Gln Ile 
            740                 745                 750         


Trp Gln Thr Leu Leu Val Ile Lys Asp Ile Lys Gln Val Met Lys His 
        755                 760                 765             


Asp Pro Lys Arg Ile Phe Ile Glu Met Ala Arg Glu Lys Gln Glu Ser 
    770                 775                 780                 


Lys Thr Thr Lys Ser Arg Lys Gln Val Leu Ser Glu Val Tyr Lys Asn 
785                 790                 795                 800 


Ala Glu Lys Tyr Lys Asn Leu Phe Glu Lys Leu Asn Ser Leu Thr Glu 
                805                 810                 815     


Glu Gln Leu Arg Ser Lys Lys Val Tyr Leu Tyr Phe Thr Gln Leu Gly 
            820                 825                 830         


Lys Cys Met Tyr Thr Asn Asp Ala Ile Asp Phe Glu Asn Leu Val Ser 
        835                 840                 845             


Ala Asn Ser Asn Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr 
    850                 855                 860                 


Ile Asp Asp Ser Phe Asn Asn Leu Val Leu Val Lys Lys Gly Ile Asn 
865                 870                 875                 880 


Asn Asp Lys Ser Asp Arg Tyr Pro Ile Asp Lys Asn Ile Arg Asp Asp 
                885                 890                 895     


Glu Lys Val Lys Thr Leu Trp Asn Thr Leu Leu Ser Lys Gly Leu Ile 
            900                 905                 910         


Thr Lys Glu Lys Phe Glu Arg Leu Ile Arg Ser Thr Pro Phe Ser Asp 
        915                 920                 925             


Glu Glu Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Ser Thr Lys Ala Val Ala Glu Ile Leu Ser Asn Trp Phe Pro Glu Ser 
945                 950                 955                 960 


Glu Ile Val Tyr Ser Lys Ala Lys His Ile Thr Asn Phe Arg Gln Asp 
                965                 970                 975     


Phe Glu Ile Leu Lys Val Arg Glu Leu Asn Asp Cys His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ile Val Val  Gly Asn Ala Tyr His  Thr Lys Phe 
        995                 1000                 1005             


Thr Asn  Ser Pro Tyr Arg Phe  Ile Gln Asn Lys Ala  Asn Gln Glu 
    1010                 1015                 1020             


Tyr Asn  Leu Arg Lys Leu Leu  Gln Lys Ala Lys Lys  Ile Glu Ser 
    1025                 1030                 1035             


Asn Gly  Val Ile Ala Trp Ile  Gly Gln Ser Glu Asn  Asn Pro Gly 
    1040                 1045                 1050             


Thr Ile  Ala Thr Val Lys Lys  Val Ile Ser Arg Asn  Thr Val Leu 
    1055                 1060                 1065             


Ile Ser  Arg Met Val Lys Glu  Val Asp Gly Gln Leu  Phe Asp Gln 
    1070                 1075                 1080             


Gln Leu  Met Lys Lys Gly Lys  Gly Gln Val Pro Ile  Lys Ser Ser 
    1085                 1090                 1095             


Asp Asp  Arg Leu Ile Asp Ile  Ser Lys Tyr Gly Gly  Tyr Asn Lys 
    1100                 1105                 1110             


Ala Lys  Gly Ala Tyr Phe Val  Phe Ile Lys Ser Val  Arg Arg Gly 
    1115                 1120                 1125             


Lys Thr  Ile Lys Ser Phe Glu  Tyr Ile Pro Val His  Leu Ala Lys 
    1130                 1135                 1140             


Lys Phe  Asp Cys Asn Leu Glu  Leu Leu Lys Glu Tyr  Leu Glu Ser 
    1145                 1150                 1155             


Glu Lys  Asp Leu Asn Asn Val  Glu Ile Leu Met Pro  Lys Val Met 
    1160                 1165                 1170             


Ile Asn  Ser Leu Phe Asn Tyr  Asn Gly Ser Leu Ile  Arg Ile Pro 
    1175                 1180                 1185             


Gly Arg  Tyr Asp Lys Lys Ser  Leu Leu Ile Asn Val  Asp Val Pro 
    1190                 1195                 1200             


Leu Leu  Leu Glu Ser Gln His  Ile Lys Gln Leu Lys  Val Ile Glu 
    1205                 1210                 1215             


Lys Tyr  Met Tyr Lys Lys Arg  Val Ser Lys Asn Ser  Asn Ile Leu 
    1220                 1225                 1230             


Leu Thr  Lys Phe Ala Ser Asp  Gln Leu Lys Asp Leu  Asp Ala Leu 
    1235                 1240                 1245             


Phe Asp  Val Leu Ser Tyr Lys  Leu Asn Glu Asn Ile  Tyr Asn Val 
    1250                 1255                 1260             


Ile Asn  Asp Lys Tyr Asp Lys  Leu Val Ile Cys Arg  Asp Lys Phe 
    1265                 1270                 1275             


Ile Ser  Leu Asp Thr Glu Val  Lys Cys Glu Met Ile  Phe Glu Leu 
    1280                 1285                 1290             


Leu His  Leu Phe Gln Cys Asn  Ser Gln Leu Ala Asn  Ile Thr Lys 
    1295                 1300                 1305             


Ile Gly  Ala Thr Ser Lys Phe  Gly Ser Ile Ser Met  Ser Lys Asn 
    1310                 1315                 1320             


Leu Lys  Glu Asn Asp Lys Met  Ser Ile Ile His Gln  Ser Pro Ser 
    1325                 1330                 1335             


Gly Ile  Phe Glu His Glu Ile  Glu Leu Thr Ala Leu  
    1340                 1345                 1350 


<210>  914
<211>  1350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  914

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Ala Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ser Glu Asn Glu Lys Glu Lys Ser Glu Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Gln Thr Asp 
        35                  40                  45              


Phe Glu Lys Gly Arg Pro Leu Ser Val Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Asn Leu Gln Arg Phe Lys Gln Arg Arg Lys Asn 
65                  70                  75                  80  


Leu Ile Glu Ile Leu Ile Lys Lys Gly Phe Ile Thr Asn Ile Ser Leu 
                85                  90                  95      


Leu Thr Glu Ile Gly Ser Asn Thr Thr Tyr Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ser Val Lys Glu Lys Ile Glu Lys Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Thr Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Asp Glu Glu Gly Ser Leu Ile Asp Gly Met Ser Val Ala Lys 
145                 150                 155                 160 


Glu Leu Tyr Asp Asn Asn Leu Thr Val Gly Gln Tyr Val Tyr Gln Leu 
                165                 170                 175     


Leu Glu Lys Gly Gly Lys Tyr Ile Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Phe Asp Val Ile Trp Asn Phe Gln Lys Glu Phe Tyr Pro 
        195                 200                 205             


Asn Ile Leu Thr Gly Glu Leu Tyr Lys Lys Leu Gln Gly Gln Gly Gln 
    210                 215                 220                 


Gln Asn Ser Arg Lys Leu Phe Leu Ala Ile His Gln Ile Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Lys Arg Asp Thr Val Lys Lys Gln His Tyr Lys Trp 
                245                 250                 255     


Arg Asn Asp Ala Ile Asn Thr Gln Leu Leu Ile Glu Glu Val Ala Tyr 
            260                 265                 270         


Val Leu Val Glu Ile Asn Asn Asn Leu Asn Lys Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Glu Thr 
    290                 295                 300                 


Val Gly Glu Asn Leu Tyr Lys Gln Ile Gln Glu Asn Pro Gln Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Glu Thr Gln Ala Lys Phe Tyr Ser Glu Leu Thr Lys Glu 
            340                 345                 350         


Leu Lys Glu Glu Val Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys His Leu Ile Ser Glu Cys Glu Phe Glu Lys His His 
    370                 375                 380                 


Lys Ala Ile Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp 
385                 390                 395                 400 


Gln Ile Leu Asn Asn Ile Glu Phe Lys His Lys Lys Thr Lys Glu Leu 
                405                 410                 415     


Lys Ile Leu Ser Glu Glu Leu Lys Glu Thr Leu Phe Glu Glu Leu Asn 
            420                 425                 430         


Ile Arg Gly Asn Phe Thr Ser Asp Ala Val Leu Lys Phe Val Lys Leu 
        435                 440                 445             


Asp Lys Lys Glu Trp Glu Leu Asn Tyr Lys Lys Gly Ile Glu Gly Asn 
    450                 455                 460                 


Arg Thr Asn Glu Val Leu Tyr Asn Val Tyr Gln Lys Ile Ala Glu Tyr 
465                 470                 475                 480 


Glu Gly Tyr Gly Phe Asp Trp Ser Lys Lys Ser Ala Val Glu Ile Lys 
                485                 490                 495     


Glu Glu Leu Asn Thr Ile Phe Ser Glu Ile Gly Ile Asn Ser Lys Ile 
            500                 505                 510         


Leu Asp Phe Asn Pro Glu Ile Ser Gly Asn Asp Phe Asp Lys Gln Glu 
        515                 520                 525             


Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Ala Glu Glu Asp Gly Phe 
    530                 535                 540                 


Leu Lys Gln Lys Leu Gln Asp Lys Tyr Gly Phe Lys Ser Glu Tyr Ala 
545                 550                 555                 560 


Asn Met Leu Ala Asn Val Ser Phe Gln Ser Asp Tyr Gly Ser Leu Ser 
                565                 570                 575     


Ala Arg Ala Ile Lys Lys Ile Leu Pro Asn Leu Ile Ala Gly His Glu 
            580                 585                 590         


Phe Ser Glu Ala Cys Ser Leu Ser Gly Tyr Asn His Ser Asn Ser Leu 
        595                 600                 605             


Thr Lys Glu Glu Leu Ala Asn Arg Pro Leu Lys Gly Lys Leu Glu Leu 
    610                 615                 620                 


Leu Lys Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn 
625                 630                 635                 640 


Gln Met Val Asn Leu Val Asn Gln Val Ile Glu Ser Tyr Gly Glu Ile 
                645                 650                 655     


Asp Glu Val Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Ser Ala Gln 
            660                 665                 670         


Glu Arg Ala Leu Met Thr Thr Asn Ile Asn Ala Ser Lys Thr Lys Tyr 
        675                 680                 685             


Glu Ser Phe Arg Lys Leu Leu Gln Lys Asp Phe Gly Ile Lys Asn Pro 
    690                 695                 700                 


Thr Arg Asn Asp Ile Ile Lys Tyr Arg Leu Tyr Glu Glu Leu Lys Ala 
705                 710                 715                 720 


Asn Lys Tyr His Thr Ile Tyr Thr Asn Lys Tyr Ile Pro Arg Asp Lys 
                725                 730                 735     


Leu Phe Ser Lys Glu Ile Asp Ile Glu His Ile Ile Pro Lys Ala Lys 
            740                 745                 750         


Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Ala Phe Arg Lys Pro 
        755                 760                 765             


Asn Gln Asp Lys Gly Asn Met Thr Ala Ile Asp Tyr Ile Leu Ser Tyr 
    770                 775                 780                 


Gln Ser Lys Asp Ile Asp Asn Tyr Lys Ser Arg Ile Glu Asn Leu Phe 
785                 790                 795                 800 


Lys Asn Gly Lys Gly Asp Ile Ser Lys Ala Lys Arg Asn Lys Leu Leu 
                805                 810                 815     


Met Glu Glu Lys Asp Ile Pro Asp Gly Phe Ile Asp Arg Asp Leu Arg 
            820                 825                 830         


Asn Ser Gln Tyr Ile Ala Lys Lys Ala Lys Gln Leu Leu Glu Glu Val 
        835                 840                 845             


Ile Arg Thr Val Asn Thr Thr Thr Gly Ser Val Thr Asn Lys Leu Arg 
    850                 855                 860                 


Asp Asp Trp Gly Leu Ile Asn Val Met Gln Glu Leu Asn Met Pro Lys 
865                 870                 875                 880 


Phe Lys Ala Ile Asp Glu Ser Leu Ile Glu Gln Ile Glu Lys Lys Asp 
                885                 890                 895     


Gly Thr Phe Lys Thr Arg Ile Lys Asp Trp Ser Lys Arg Asn Asp His 
            900                 905                 910         


Arg His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Arg Tyr Asn 
        915                 920                 925             


His Ile Gln Tyr Leu Asn Asn Leu Asn Ala Lys Ser Asp Lys Lys Gly 
    930                 935                 940                 


Ala Ile Tyr Gly Ile Gln Glu Leu Glu Thr Tyr Val Asn Ser Lys Gly 
945                 950                 955                 960 


Lys Arg Lys Phe Ile Lys Pro Met His Asn Phe Arg Glu Glu Ala Lys 
                965                 970                 975     


Lys His Ile Glu Asp Ile Leu Ile Ser Phe Lys Ala Lys Asn Lys Val 
            980                 985                 990         


Val Thr Lys Asn Ile Asn Lys Ile  Lys Ser Lys Asp Gly  Phe Ile Lys 
        995                 1000                 1005             


Lys Val  Gln Leu Thr Pro Arg  Gly Gln Leu His Lys  Glu Thr Val 
    1010                 1015                 1020             


Tyr Gly  Lys Ile Asn Glu Val  Val Val Lys Glu Glu  Lys Ile Asn 
    1025                 1030                 1035             


Ala Lys  Phe Asp Leu Glu Lys  Ile Asn Lys Val Thr  Lys Pro Ile 
    1040                 1045                 1050             


Tyr Arg  Asn Ala Leu Leu Lys  Arg Leu Gln Glu Phe  Asn Gly Glu 
    1055                 1060                 1065             


Pro Lys  Lys Ala Phe Thr Gly  Lys Asn Ser Leu Ser  Lys Asn Pro 
    1070                 1075                 1080             


Ile Tyr  Leu Asp Val Ala Gln  Thr Lys Thr Leu Pro  Glu Ser Val 
    1085                 1090                 1095             


Lys Val  Gln Phe Phe Glu Glu  Asn Tyr Thr Ile Arg  Lys Glu Ile 
    1100                 1105                 1110             


Ser Pro  Asp Leu Lys Ile Asp  Lys Ile Ile Asp Lys  Gly Ile Gln 
    1115                 1120                 1125             


Arg Ile  Leu Leu Lys Arg Leu  Asp Glu Phe Asn Gly  Lys Pro Lys 
    1130                 1135                 1140             


Glu Ala  Phe Ser Asn Leu Glu  Lys Asn Pro Ile Trp  Leu Asn Lys 
    1145                 1150                 1155             


Glu Lys  Gly Ile Ala Ile Lys  Arg Val Lys Ile Lys  Gly Val Lys 
    1160                 1165                 1170             


Asn Val  Glu Ser Leu His Asn  Lys Arg Asp His Leu  Gly Asn Glu 
    1175                 1180                 1185             


Ile Leu  Gln Lys Gly Lys Ser  Ala Pro Asn Asp Tyr  Val Ser Thr 
    1190                 1195                 1200             


Gly Asn  Asn His His Val Ala  Ile Tyr Gln Asp Lys  Asn Gly Lys 
    1205                 1210                 1215             


Leu Gln  Glu Lys Val Val Ser  Phe Tyr Glu Ala Val  Ala Arg Val 
    1220                 1225                 1230             


Asn Asn  Lys Leu Pro Ile Ile  Asp Gln Asp Phe Asn  Lys Asp Leu 
    1235                 1240                 1245             


Gly Trp  Lys Phe Leu Phe Thr  Met Lys Gln Asn Glu  Met Phe Val 
    1250                 1255                 1260             


Phe Pro  Ser Glu Asp Phe Lys  Pro Leu Glu Thr Asp  Leu Met Asp 
    1265                 1270                 1275             


Lys Lys  Asn Asn Ser Lys Ile  Ser Lys Asn Leu Phe  Arg Val Gln 
    1280                 1285                 1290             


Lys Leu  Gly Asn Lys Asp Tyr  Thr Phe Arg His His  Leu Glu Thr 
    1295                 1300                 1305             


Lys Ile  Glu Asp Ile Lys Lys  Leu Arg Asp Phe Thr  Tyr Ile Arg 
    1310                 1315                 1320             


Lys Arg  Asn Pro Glu Ser Ile  Lys Asn Ile Ile Lys  Val Arg Ile 
    1325                 1330                 1335             


Asn His  Leu Gly Asn Ile Val  His Val Gly Glu Tyr  
    1340                 1345                 1350 


<210>  915
<211>  1351
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  915

Met Ser Lys Lys Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Cys Lys Tyr Ala Gly 
            20                  25                  30          


Lys Arg Met Trp Gly Ile Arg Leu Phe Glu Gly Gly Glu Thr Ala Glu 
        35                  40                  45              


Gln Arg Arg Ile Ser Arg Ser Asn Arg Arg Arg Leu Ser Arg Lys Lys 
    50                  55                  60                  


Gln Arg Ile Asp Leu Leu Gln Glu Leu Phe Ala Glu Glu Ile Ala Lys 
65                  70                  75                  80  


Met Asp Pro Thr Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Leu 
                85                  90                  95      


Glu Asp Lys Ser Thr Gly Glu Lys Phe Pro Leu Phe Arg Asp Lys Asp 
            100                 105                 110         


Tyr Thr Asp Ile Gln Tyr Tyr Lys Glu Tyr Pro Thr Met Tyr His Leu 
        115                 120                 125             


Arg Lys Glu Leu Ile Glu Asn Ser Lys Val His Asp Ile Arg Leu Val 
    130                 135                 140                 


Tyr Leu Ala Cys His His Ile Leu Lys Asn Arg Gly His Phe Leu Ile 
145                 150                 155                 160 


Asn Gly Ser Phe Ser Asp Val Lys Lys Leu Ser Ser Val Cys Glu Ser 
                165                 170                 175     


Met Leu Asn Ser Phe Asn Gly Ile Ser Lys Tyr Gln Ile Glu Ile Asn 
            180                 185                 190         


Asp Ile Glu Arg Leu Glu Glu Ile Leu Ser Asp Arg Lys Lys Gln Lys 
        195                 200                 205             


Ser Val Lys Ala Lys Glu Leu Ile Gln Ile Phe Glu Cys Gly Pro Cys 
    210                 215                 220                 


Glu Asp Lys Thr Asp Glu Lys Thr Cys Lys Ala Ile Val Ser Asn Leu 
225                 230                 235                 240 


Cys Lys Trp Leu Thr Gly Leu Lys Gly Asn Ile Asn Ser Ile Phe Asp 
                245                 250                 255     


Met Gln Phe Glu Glu Ile Asp Thr Ser Ser Phe Ser Phe Thr Glu Pro 
            260                 265                 270         


Lys Tyr Glu Asp Thr Ile Ser Ala Ala Leu Glu Glu Lys Tyr Pro Asp 
        275                 280                 285             


Tyr Ala Asp Val Ile Gln Lys Ile Lys Ala Val Tyr Asp Trp Ser Val 
    290                 295                 300                 


Leu Val Glu Ile Met Ala Gly Glu Asp Tyr Leu Ser Phe Ala Lys Val 
305                 310                 315                 320 


Asn Ala Tyr Asn Lys His Lys Glu Asn Leu Cys Ile Leu Lys Glu Val 
                325                 330                 335     


Ile Arg Lys Tyr Cys Asp Ser Glu Ile Tyr His Lys Phe Phe Asn Gly 
            340                 345                 350         


Phe Asp Glu Lys Asn Gly Tyr Gly Asn Tyr Ile Gly Ser Ile Tyr Lys 
        355                 360                 365             


Asn Gly His Ser Tyr Ala Met Lys Lys Cys Ser Thr Glu Asp Phe Tyr 
    370                 375                 380                 


Lys Thr Leu Gly Lys Ile Leu Asp Lys Ile Gln Pro Glu Lys Glu Asp 
385                 390                 395                 400 


Glu Asn Val Leu Glu Glu Leu Lys Val Glu Val Lys Asn Gln Thr Leu 
                405                 410                 415     


Leu Pro Leu Ala Arg Thr Thr Ala Asn Gly Thr Ile Pro Asn Gln Val 
            420                 425                 430         


His Ile Gln Glu Leu Arg Ala Ile Leu Asp Asn Ala Lys Lys Tyr Leu 
        435                 440                 445             


Pro Phe Leu Asn Glu Glu Asp Glu Tyr Gly Thr Val Ala Glu Lys Ile 
    450                 455                 460                 


Ile Ser Ile Ala Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ser 
465                 470                 475                 480 


Thr Arg His Lys Glu Gln Gly Ala Asn Ser Trp Ile Val Arg Lys Gln 
                485                 490                 495     


Glu Gly Arg Ile Tyr Pro Trp Asn Phe Glu Glu Met Val Asp Leu Glu 
            500                 505                 510         


Gln Ser Asn Lys Glu Phe Ile Gln Arg Met Thr Asn Lys Cys Thr Tyr 
        515                 520                 525             


Leu Ile Gly Glu Asp Val Leu Pro Lys Asn Ser Leu Leu Tyr Ser Arg 
    530                 535                 540                 


Tyr Met Val Leu Asn Glu Leu Asn Asn Leu Arg Ile Arg Gly Asn Lys 
545                 550                 555                 560 


Ile Ser Val Lys Leu Lys Gln Glu Leu Tyr Lys Glu Leu Phe Cys Thr 
                565                 570                 575     


Lys Ala Lys Val Thr Gly Lys Gly Leu Phe Asn Tyr Leu Lys Lys Glu 
            580                 585                 590         


Asp Glu Glu Leu Ala Leu Glu Asp Ile Ser Gly Phe Asp Ile Asp Phe 
        595                 600                 605             


Lys Ser Ser Leu Thr Ser Tyr Leu Asp Phe Lys Lys Gln Ile Leu Gly 
    610                 615                 620                 


Glu Glu Ile Glu Lys Asp Lys Tyr Lys Gly Ile Val Glu Asn Ile Ile 
625                 630                 635                 640 


Lys Trp Lys Thr Ile Tyr Asp Asp Asp Ser Lys Met Met Lys Lys Met 
                645                 650                 655     


Ile Glu Arg Glu Tyr Pro Asn Val Phe Ser Lys Asp Lys Ile Lys Lys 
            660                 665                 670         


Ile Cys Arg Phe Lys Tyr Ser Gly Trp Gly Asn Phe Ser Leu Ser Phe 
        675                 680                 685             


Leu Asn Gly Ile Arg Gly Ala Asp Arg Glu Thr Gly Glu Ser Phe Thr 
    690                 695                 700                 


Ile Ile Glu Ala Leu Trp Lys Thr Asn Tyr Asn Leu Met Gln Leu Leu 
705                 710                 715                 720 


Ser Lys Gln Phe Thr Phe Arg Glu Glu Ile Asp Leu Ile Asn Ala Glu 
                725                 730                 735     


Lys Val Gly Lys Ile Asn Lys Val Ser Tyr Asp Asn Thr Val Lys Asp 
            740                 745                 750         


Leu Ile Val Ser Pro Ala Asn Lys Arg Ala Ile Trp Gln Thr Val Gln 
        755                 760                 765             


Ile Thr Glu Glu Ile Lys Lys Val Met Lys Cys Glu Pro Glu Arg Ile 
    770                 775                 780                 


Phe Ile Glu Met Ala Arg Gly Gly Glu Lys Glu Lys Lys Arg Thr Val 
785                 790                 795                 800 


Ser Arg Lys Ala Arg Leu Leu Glu Leu Tyr Ala Ala Cys Lys Asp Asp 
                805                 810                 815     


Val Arg Asp Trp Thr Lys Glu Ile Glu Asp Arg Glu Glu Arg Glu Phe 
            820                 825                 830         


Asn Ser Lys Lys Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Cys Met 
        835                 840                 845             


Tyr Ser Gly Glu Glu Ile Asn Ile Asp Glu Leu Met Gln Lys Asn Ser 
    850                 855                 860                 


Lys Trp Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp 
865                 870                 875                 880 


Ser Leu Asp Asn Met Val Leu Ala Lys Lys Val Ile Asn Asn Ser Lys 
                885                 890                 895     


Gln Asn Lys Leu Leu Ser Ser Asp Ile Arg Ile Lys Met Lys Lys Tyr 
            900                 905                 910         


Trp Asn Ala Leu Tyr Lys Gly Asn Phe Ile Ser Lys Lys Lys Tyr Asp 
        915                 920                 925             


Arg Leu Thr Arg Thr Glu Asp Phe Thr Asn Glu Glu Leu Ser Gly Phe 
    930                 935                 940                 


Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ser Ser Lys Ala Val Ala 
945                 950                 955                 960 


Glu Leu Leu Asn Arg Leu Tyr Glu His Ser Glu Ile Val Tyr Val Lys 
                965                 970                 975     


Ala Gly Leu Val Ser Asp Phe Arg His Asp Asn Lys Leu Leu Lys Ser 
            980                 985                 990         


Arg Arg Ile Asn Asp Tyr His His  Ala Lys Asp Ala Phe  Leu Asn Ile 
        995                 1000                 1005             


Val Val  Gly Asn Val Tyr Asn  Ala Lys Phe Thr Ser  Asn Pro Arg 
    1010                 1015                 1020             


Lys Trp  Ile Lys Glu Asn Arg  Gly Thr Asn Tyr Ser  Ile Arg Arg 
    1025                 1030                 1035             


Val Phe  Asp Tyr Asn Val Lys  Arg Gly Asn Thr Val  Val Trp Gln 
    1040                 1045                 1050             


Gly Val  Glu Asp Gly Gly His  Ser Ile Asp Glu Ile  Arg Lys Thr 
    1055                 1060                 1065             


Met Ala  Arg Asn Asp Ile Leu  Tyr Thr Glu Tyr Thr  Tyr Cys Asp 
    1070                 1075                 1080             


Lys Gly  Glu Leu Phe Asn Ala  Thr Leu Ile Lys Lys  Gly Asn Asp 
    1085                 1090                 1095             


Lys Ala  Ile Asn Leu Lys Lys  Ala Leu Asp Ser Val  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Tyr  Asn Ser Pro Lys Thr  Ser Ala Phe Ala Phe  Ile Glu Phe 
    1115                 1120                 1125             


Asp Gly  Lys Lys Lys Glu Arg  Lys Asn His Ile Val  Glu Ile Pro 
    1130                 1135                 1140             


Val Tyr  Ile Val Asn Ile Ala  Lys Arg Gln Pro Asp  Thr Ile Lys 
    1145                 1150                 1155             


Glu Tyr  Leu Glu Gln Lys Lys  Gly Tyr Lys Asn Val  Val Val Lys 
    1160                 1165                 1170             


Lys Tyr  Pro Ile Lys Lys Asn  Ala Leu Ile Lys Ile  Asp Gly Tyr 
    1175                 1180                 1185             


Leu Ala  Arg Leu Arg Gly Ala  Asn Glu Thr Asp Ile  Leu Leu Lys 
    1190                 1195                 1200             


Asn Ala  Val Gln Leu Ile Leu  Asn Asp Lys Tyr Tyr  Glu Ile Ile 
    1205                 1210                 1215             


Arg Arg  Val Glu Lys Tyr Leu  Glu Tyr Asn Val Arg  Tyr Glu Ala 
    1220                 1225                 1230             


Glu Ser  Met Phe Glu Gly Phe  Asp His Ala Asp Leu  Asn Ser Leu 
    1235                 1240                 1245             


Tyr Asp  Glu Leu Thr Ser Lys  Leu Glu Thr Ser Ile  Tyr Lys Lys 
    1250                 1255                 1260             


Arg Pro  Ala Asn Gln Leu Asn  Ala Leu Glu Lys Gly  Lys Glu Lys 
    1265                 1270                 1275             


Phe Lys  Asn Ile Gln Thr Leu  Arg Glu Lys Ala Gln  Ile Ile Lys 
    1280                 1285                 1290             


Glu Met  Leu Ile Met Tyr Arg  Cys Asp Ala Ala Thr  Val Ala Asn 
    1295                 1300                 1305             


Leu Lys  Ala Ile Gly Gly Ser  Ala Asn Ala Gly Ser  Met Lys Ile 
    1310                 1315                 1320             


Lys Lys  Asn Thr Leu Thr Ser  Arg Ser Leu Val Leu  Val Asn Gln 
    1325                 1330                 1335             


Ser Ile  Thr Gly Leu Phe Glu  Thr Glu Glu Glu Leu  Val 
    1340                 1345                 1350     


<210>  916
<211>  1351
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  916

Met Tyr Lys Lys Tyr Gly Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Trp Ser Val Val Asp Glu Tyr Gly His Ile Ile Arg Val Lys Gly 
            20                  25                  30          


Gln Thr Gly Leu Gly Val Arg Leu Phe Arg Glu Gly Gln Pro Ala Ala 
        35                  40                  45              


Asp Arg Arg Thr Phe Arg Thr Thr Arg Arg Arg Leu Gly Arg Arg Arg 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Ile Phe Asp Pro Tyr Ile Ala Ala 
65                  70                  75                  80  


Gln Asp Pro Asn Phe Phe Ala Arg Gln Lys Ala Ser Ala Ile Ala Pro 
                85                  90                  95      


Gly Asp Ser Arg Phe Lys Ala Val Tyr Arg Leu Phe Asn Asp Gln Thr 
            100                 105                 110         


Asp Arg Gln Phe Tyr Asp Lys Tyr Pro Thr Val Tyr His Leu Arg Gln 
        115                 120                 125             


Lys Leu Met Thr Glu Lys Arg Gln Phe Asp Ile Arg Glu Ile Tyr Leu 
    130                 135                 140                 


Ala Val His His Ile Val Lys Tyr Arg Gly His Phe Leu Thr Gly Gly 
145                 150                 155                 160 


Ala Ala Lys Asn Phe Lys Pro Gly Lys Leu Asp Leu Asn Thr Tyr Phe 
                165                 170                 175     


Ala Thr Ile Asn Ala Gln Leu Ala Ala Ile Phe Met Asp Ser Gln Val 
            180                 185                 190         


Glu Leu Pro Gln Leu Asp Trp Asp Ala Val Val Ser Leu Leu Thr Asn 
        195                 200                 205             


Asp Gln Glu Ser Pro Ser Asp Arg Gln Lys Ala Leu Thr Lys Met Leu 
    210                 215                 220                 


Val Ala Gln Gly Asp Ala Thr Gln Lys Arg Phe Tyr Thr Glu Phe Ser 
225                 230                 235                 240 


Lys Ala Leu Leu Gly Leu Lys Ala Lys Leu Tyr Val Leu Thr Gly Phe 
                245                 250                 255     


Glu Gly Asp Lys Thr Asp Gln Lys Ala Leu Thr Leu Ser Leu Glu Thr 
            260                 265                 270         


Leu Asp Asp His Gln Asp Asp Val Thr Gln Leu Leu Gly Asp Glu Gln 
        275                 280                 285             


Arg Gln Leu Val Glu Leu Leu Ala Arg Val Gln Ser Ala Ile Gln Leu 
    290                 295                 300                 


Ala Ile Ile Ile Pro Asn Gly Lys Thr Phe Ser Gly Gln Met Val Gln 
305                 310                 315                 320 


Gln Tyr Gln Thr His Tyr Asp Gln Leu Arg Leu Leu Lys Asp Tyr Arg 
                325                 330                 335     


Glu Gln Leu Thr Asp Gln Lys His Arg Arg Ala Leu Lys Gln Ala Tyr 
            340                 345                 350         


Ala Asp Tyr Leu Ala Asn Asp Gln Asp Gln Asp Ala Leu Val Lys Ala 
        355                 360                 365             


Val Glu Lys Val Leu Lys Gln Asn Gln Pro Leu Ser Thr Val Gly Val 
    370                 375                 380                 


Arg Leu Gln Thr Ala Ile Asp Ala Gly Asp Phe Leu Pro Lys Gln Arg 
385                 390                 395                 400 


Thr Lys Ala Asn Ser Val Ile Pro His Gln Ile His Gln Gln Glu Leu 
                405                 410                 415     


Asp Arg Ile Ile Glu Ala Gln Ser Val Tyr Tyr Pro Trp Leu Ala Glu 
            420                 425                 430         


Glu Asn Pro Val Ala Ala His Arg Ser Tyr Ala Pro Tyr Lys Leu Asp 
        435                 440                 445             


Glu Leu Val Asp Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Val Gln 
    450                 455                 460                 


Pro Gln Ala Asp Lys Ala Ala Lys Glu Thr Lys Phe Ala Trp Met Thr 
465                 470                 475                 480 


Arg Lys Glu Ala Gln Ser Thr Thr Pro Ile Thr Pro Trp Asn Phe Glu 
                485                 490                 495     


Gln Glu Val Asp Arg Asn Ala Ser Ala Asn Ala Phe Ile Gln Arg Met 
            500                 505                 510         


Lys Thr Thr Asp Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Lys Asn 
        515                 520                 525             


Ser Leu Leu Tyr Gln Arg Phe Glu Val Leu Asn Glu Leu Asn Asn Val 
    530                 535                 540                 


Arg Val Asp Gly Lys Leu Leu Thr Ile Ala Glu Lys Gln Arg Leu Phe 
545                 550                 555                 560 


Gln Asp Leu Phe Val Asp Arg Ser Gln Ala Ser Val Thr Ile Ala Gln 
                565                 570                 575     


Leu Gln Ala Asn Leu Ile Ala His Gly Ala Ala Lys Asp Arg Val Glu 
            580                 585                 590         


Ile Lys Gly Leu Ala Asp Pro Lys Arg Phe Leu Ser Ser Leu Thr Thr 
        595                 600                 605             


Tyr His Asp Tyr Ala Gln Ile Ile Pro Glu Ala Leu Glu Gln Pro Asp 
    610                 615                 620                 


Lys Arg Ala Asp Ile Glu Lys Ile Ile Asn Trp Ser Thr Ile Phe Glu 
625                 630                 635                 640 


Asp Thr Ser Ile Phe Lys Glu Lys Leu Gln Glu Ile Ala Trp Leu Thr 
                645                 650                 655     


Pro Asp Gln Arg Arg Gln Leu Ser Gln Lys Arg Tyr Arg Gly Trp Gly 
            660                 665                 670         


Gln Leu Ser Asp Lys Leu Leu Thr Gln Leu Arg Asp Ala Thr Gly Gln 
        675                 680                 685             


Ser Val Met Asp Leu Leu Trp Thr Glu Pro Asn Asn Phe Met Gln Ile 
    690                 695                 700                 


Ile Arg Arg Thr Glu Phe Gln Gln Ala Ile Ala Arg Val Asn Gln Thr 
705                 710                 715                 720 


Asp Leu Lys Gln Gln Asp Leu Ala Thr Thr Ile Asn Asp Leu Tyr Thr 
                725                 730                 735     


Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Leu Leu Val Val Ala 
            740                 745                 750         


Asp Ile Gln Thr Ala Met His Glu Val Ala Pro Ser Trp Leu Phe Ile 
        755                 760                 765             


Glu Ala Ala Arg Gly Pro Glu Gln Asn Pro Lys Arg Lys Leu Ala Arg 
    770                 775                 780                 


Gln Gln Gln Leu Leu Glu Ala Tyr Lys Thr Ser Ala Lys Glu Ile Val 
785                 790                 795                 800 


Asn Glu Ala Val Lys Glu Lys Leu Leu Ser Ala Ile Asp Asp His Gln 
                805                 810                 815     


Lys Phe Glu Asp Gln Val Val Leu Tyr Phe Gln Gln Asn Gly Leu Asp 
            820                 825                 830         


Met Tyr Thr Gly Lys Pro Leu Asp Phe Glu Arg Leu Ser Lys Tyr His 
        835                 840                 845             


Ile Asp His Ile Ile Pro Gln Ser Val Val Lys Asp Asp Ser Leu Asp 
    850                 855                 860                 


Asn Arg Val Leu Thr Thr Pro Glu Glu Asn Glu Arg Lys Gly Ala Arg 
865                 870                 875                 880 


Val Pro Gly Pro Leu Tyr Ala Ser Gln Gln Gly Arg Trp Arg Leu Leu 
                885                 890                 895     


Gln Arg Ala Gly Leu Ile Ser Gln Arg Lys Leu Asp His Leu Leu Leu 
            900                 905                 910         


Arg Pro Glu Glu Leu Asp Gln Gln Ala Thr Gly Phe Val Ala Arg Gln 
        915                 920                 925             


Leu Val Glu Thr Arg Gln Val Ile Lys Leu Ile Thr Asn Ile Leu Ser 
    930                 935                 940                 


Gln Gln Tyr Asp Pro Glu Gln Thr Lys Leu Val Ser Val Lys Ala Gly 
945                 950                 955                 960 


Leu Ser His Gln Phe Arg Thr Arg Leu Asn Leu Pro Lys Leu Arg Glu 
                965                 970                 975     


Leu Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Ala Ala Arg Ile 
            980                 985                 990         


Gly Thr Tyr Leu Leu Gln Arg Tyr  Pro Asn Leu Glu Arg  Phe Phe Val 
        995                 1000                 1005             


Tyr Gly  Gln Tyr Gln Lys Thr  Arg Phe Asp Leu Lys  Arg His Phe 
    1010                 1015                 1020             


Asn Phe  Ile Gly Glu Met Leu  Lys Gly Lys Pro Glu  Asp Ala Val 
    1025                 1030                 1035             


Ile Val  Lys Ala Pro His Ser  Glu Thr Val Ile Trp  Asp Lys Gln 
    1040                 1045                 1050             


Asp Glu  Val Lys Gln Leu Lys  Lys Ile Met Glu Tyr  Arg His Leu 
    1055                 1060                 1065             


Leu Val  Thr His Glu Val Tyr  Asp Asn His Gly Ala  Leu Phe Asn 
    1070                 1075                 1080             


Gln Thr  Ile His Pro Ala Lys  Asn Pro Lys Asn Arg  Lys Leu Ile 
    1085                 1090                 1095             


Pro Arg  Lys Thr Thr Gln Ser  Thr Glu Leu Tyr Gly  Gly Tyr Ser 
    1100                 1105                 1110             


Gly Glu  Thr Thr Gly Tyr Leu  Ala Ile Ala Arg Thr  Val Thr Lys 
    1115                 1120                 1125             


Asn Lys  Ile Ser Tyr Gln Val  Leu Arg Val Pro Thr  Arg Leu Val 
    1130                 1135                 1140             


Gly Arg  Leu Gln Arg Ser Ser  Glu Ser Ala Gln Arg  Gln Ile Leu 
    1145                 1150                 1155             


Thr Asp  Trp Phe Ala Pro Ser  Phe Val Ser Arg Gly  Lys Gln Gln 
    1160                 1165                 1170             


Pro Phe  Glu Ile Val Leu Arg  His Val Phe Lys Asn  Gln Thr Val 
    1175                 1180                 1185             


Ile Asp  Asp Ile His Glu Gln  Ser His Arg Phe Gly  Leu Arg Ser 
    1190                 1195                 1200             


Asp Thr  Tyr Tyr Cys Asn Gln  Gln Gln Leu Val Leu  Ser Leu Glu 
    1205                 1210                 1215             


Gln Gln  Arg Met Ile Ile Asp  Gln Lys Leu Asn Asn  Thr Asn Lys 
    1220                 1225                 1230             


Leu Met  Val Val Tyr Glu Ala  Val Cys Asp Gln Val  Ala Lys Tyr 
    1235                 1240                 1245             


Phe Pro  Leu Tyr Arg Met Asn  His Phe Lys Glu Ser  Leu Leu Glu 
    1250                 1255                 1260             


Ala Gly  Pro Lys Leu Ala Glu  Leu Pro Ile Asn Thr  Val Gln Ser 
    1265                 1270                 1275             


Lys Asn  Gly Lys Leu Lys Leu  Gly Gln Arg Ser Val  Leu Leu Gln 
    1280                 1285                 1290             


Thr Met  Gln Gly Leu His Ala  Asn Pro Glu Thr Lys  Asp Leu Ser 
    1295                 1300                 1305             


Ile Ile  Gly Cys Pro Lys Tyr  Phe Gly Lys Leu Gln  Val Ser Ser 
    1310                 1315                 1320             


Gly Ile  Lys Leu Thr Glu Asn  Ala Val Leu Ile His  Glu Ser Pro 
    1325                 1330                 1335             


Thr Gly  Leu Phe Gln Arg Arg  Val Arg Leu Ser Asp  Leu 
    1340                 1345                 1350     


<210>  917
<211>  1351
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  917

Met Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp Ala 
1               5                   10                  15      


Val Val Asn Glu Ala Glu Asn Glu Arg Glu Ser Ser Ser Ile Ile Arg 
            20                  25                  30          


Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Gln Asn Asn Phe 
        35                  40                  45              


Glu Lys Gly Lys Pro Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys Arg 
    50                  55                  60                  


Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Ala Asn Leu 
65                  70                  75                  80  


Val Ser Leu Leu Lys Thr Gln Gly Trp Ile Thr Asp Glu Thr Leu Leu 
                85                  90                  95      


Ser Glu Gln Gly Asn Asn Ser Thr Phe Glu Thr Tyr Arg Leu Arg Ala 
            100                 105                 110         


Glu Ala Ala Thr Lys Glu Ile Thr Leu Glu Gln Phe Ala Arg Val Leu 
        115                 120                 125             


Leu Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Met Lys 
    130                 135                 140                 


His Asp Glu Asp Gly Ser Phe Val Asn Asn Leu Asp Ile Ala Arg Glu 
145                 150                 155                 160 


Leu Tyr Asp Ser Gly Leu Thr Pro Gly Gln Tyr Ala Tyr Asn Leu Leu 
                165                 170                 175     


Lys Asp Gly Lys Lys Tyr Ile Pro Asp Phe Tyr Arg Ser Asp Leu Gln 
            180                 185                 190         


Glu Glu Leu Asp Lys Ile Trp Ala Tyr Gln Tyr Glu Tyr His Glu Asn 
        195                 200                 205             


Ile Leu Thr His Ser Leu Arg Ser Ala Ile Ile Asp Gln Ser Lys Thr 
    210                 215                 220                 


Gly Thr Thr Lys Ile Phe Leu Lys Thr His Gly Ile Phe Thr Ala Asp 
225                 230                 235                 240 


Ile Lys Gly Asn Arg Asn Glu Arg Asn Lys Gln Leu Phe Glu Leu Arg 
                245                 250                 255     


Cys Gly Ala Leu Glu Arg Ala Leu Thr Ala Glu Glu Leu Ala Thr Val 
            260                 265                 270         


Ile Ala Glu Val Asn Gly Gln Ile Asn Ser Ser Ser Gly Tyr Leu Gly 
        275                 280                 285             


Ala Ile Ser Asp Arg Ser Lys Ala Leu Phe Ile Asn His Gln Thr Val 
    290                 295                 300                 


Gly Gln Tyr Leu Met Gln Gln Leu Asp Arg Asp Pro Asn Lys Ser Leu 
305                 310                 315                 320 


Lys Asn Gln Pro Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Gln 
                325                 330                 335     


Leu Trp Glu Thr Gln Ala His Tyr His Pro Glu Leu Thr Ala Ala Leu 
            340                 345                 350         


Lys His Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Arg Leu Lys 
        355                 360                 365             


Ser Gln Lys Trp Leu Val Ala Asn Cys Pro Phe Glu His Arg Lys Val 
    370                 375                 380                 


Cys Pro Lys Ser Ser Pro Leu Phe Gln His Phe Arg Ile Trp Gln Val 
385                 390                 395                 400 


Leu Asn Asn Val Thr Val Thr Val Asp Asn Lys Thr Tyr Val Leu Asn 
                405                 410                 415     


Glu Glu Gln Lys Ser Ile Leu Ser Lys Glu Leu Leu Tyr Lys Glu Lys 
            420                 425                 430         


Ile Ser Lys Arg Asp Ala Leu Lys Met Leu Phe Val Lys Ala Lys Gly 
        435                 440                 445             


Val Asp Met Asn Phe Ala Ser Leu Glu Gly Asp Arg Thr Met Ser Asn 
    450                 455                 460                 


Leu Leu Lys Ala Ala Gln Glu Ile Val Ser Ile Ser Gly His Gly Asp 
465                 470                 475                 480 


Tyr Asp Phe Gly Lys Met Pro Ala Asp Asp Ile His Asn Ile Ile Arg 
                485                 490                 495     


Ser Val Phe Gly Gly Leu Gly Phe Ala Thr Asp Trp Leu Asn Leu Asp 
            500                 505                 510         


Thr Asp Ala Asp Asp Leu Glu Gln Glu Ser Gln Met Arg Leu Trp His 
        515                 520                 525             


Leu Ile Tyr Ser Phe Glu Gly Asp Asn Ser Thr Thr Gly Asp Thr Lys 
    530                 535                 540                 


Leu Val Glu Arg Leu Met Ser Leu Leu Asn Leu Pro Gln Glu Tyr Ala 
545                 550                 555                 560 


Arg Leu Phe Ala Asn Val His Leu Glu Asp Asp Tyr Cys Gly Ile Ser 
                565                 570                 575     


Ser Lys Ala Leu Lys Arg Ile Leu Pro Phe Met Lys Ala Gly His Ile 
            580                 585                 590         


Tyr Ser Glu Ala Cys Ala Leu Ala Gly Tyr Lys His Ser Glu Gln Ser 
        595                 600                 605             


Leu Thr Arg Glu Glu Asn Lys Asn Arg Pro Leu Lys Asp His Leu Glu 
    610                 615                 620                 


Gln Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu 
625                 630                 635                 640 


Asn Gln Met Val Asn Val Val Asn Ser Ile Ile Asp Thr Tyr Gly Arg 
                645                 650                 655     


Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Asn Asn Ala 
            660                 665                 670         


Leu Gly Arg Gln Ala Ile Phe Glu Ala Ile Asn Arg Asn Thr Lys Glu 
        675                 680                 685             


Ser Glu Arg Ile Gln Gln Ile Leu Lys Lys Glu Phe Gly Leu Thr His 
    690                 695                 700                 


Val Ser Arg Asn Asp Ile Ile Arg Tyr Arg Leu Tyr Gln Glu Leu Ser 
705                 710                 715                 720 


Gly Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Ile Tyr Ile Pro Gln Glu 
                725                 730                 735     


Lys Leu Phe Ser Lys Glu Ile Asp Ile Glu His Ile Ile Pro Gln Ala 
            740                 745                 750         


Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu Tyr Arg Asp 
        755                 760                 765             


Val Asn Ile Lys Lys Ser Asn Thr Thr Ala Ile Asp Phe Val Arg Asn 
    770                 775                 780                 


Glu Tyr Gly Glu Asn Glu Val Glu Gln Tyr Lys Lys Arg Val Asn Asp 
785                 790                 795                 800 


Leu Leu Lys Ser Gly Ala Ile Ser Gln Ala Lys Ala Lys Lys Leu Lys 
                805                 810                 815     


Met Thr Glu Ala Glu Ile Pro Asn Asp Phe Ile Glu Arg Asp Leu Arg 
            820                 825                 830         


Asn Thr Gln Tyr Ile Ser Arg Lys Ala Gln Glu Met Leu Arg Asp Val 
        835                 840                 845             


Ala Arg Val Val Thr Ala Thr Thr Gly Ser Val Thr Asp Arg Leu Arg 
    850                 855                 860                 


Gln Asp Trp Gln Leu Val Asp Val Met Gln Glu Leu Asn Trp Asp Lys 
865                 870                 875                 880 


Tyr Glu Arg Gln Gly Leu Thr Thr Val Ile Asn Gly Arg Asp Gly Gln 
                885                 890                 895     


Gln Ile Arg Arg Ile Thr Asp Trp Thr Lys Arg Asn Asp His Arg His 
            900                 905                 910         


His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr Lys Pro Ala Tyr Ile 
        915                 920                 925             


Ser Tyr Leu Asn His Leu Asn Ala Arg Ser Glu Asp Asp Ile Arg Ser 
    930                 935                 940                 


Ile Glu Arg Arg Phe Leu Glu Arg Asp Ala Asn Gly His Leu Arg Phe 
945                 950                 955                 960 


Ile Ser Pro Ile Ala His Asn Phe Arg Ser Glu Ala Lys Arg His Leu 
                965                 970                 975     


Glu Met Ile Leu Val Ser Ile Lys Ala Lys Asn Lys Val Val Thr Arg 
            980                 985                 990         


Asn Asn Asn Thr Thr Lys Thr Lys  Gly Gly Arg Arg Asn  Arg Ile Gln 
        995                 1000                 1005             


Leu Thr  Pro Arg Gly Gln Leu  His Asp Ala Thr Val  Tyr Gly Ser 
    1010                 1015                 1020             


Ile Leu  Leu Pro His Thr Lys  Thr Ile Ala Val Asp  Gly Lys Met 
    1025                 1030                 1035             


Thr Ala  Glu Ile Ile Gly Gln  Val Gln Asn Pro Val  Phe Arg Gln 
    1040                 1045                 1050             


Ala Leu  Ser Gln Arg Leu Glu  Ala Cys Gly Asn Asp  Pro Lys Lys 
    1055                 1060                 1065             


Ala Phe  Thr Gly Lys Asn Ser  Leu Glu Lys Ala Pro  Val Trp Ile 
    1070                 1075                 1080             


Asp Gln  Ala His Thr Glu His  Val Pro Ala Glu Val  Thr Ile Gln 
    1085                 1090                 1095             


Arg Leu  Glu Pro Gln His Thr  Ile Arg Arg Ala Val  Asn Lys Asp 
    1100                 1105                 1110             


Leu Asn  Ile Asn Lys Val Leu  Asp Lys Gly Ile Gln  His Ile Leu 
    1115                 1120                 1125             


Gln Ala  Arg Leu Asp Ala Tyr  Gly Gly Asp Ala Ala  Lys Ala Phe 
    1130                 1135                 1140             


Ala Asn  Leu Asp Asn Asn Pro  Ile Trp Leu Asn Gln  Glu Lys Gly 
    1145                 1150                 1155             


Ile Ala  Ile Lys Arg Val Thr  Thr Ala Glu Asn Ile  Ser Gly Glu 
    1160                 1165                 1170             


Pro Leu  His His Lys His Asp  Lys Glu Gly Arg Pro  Ile Leu Asp 
    1175                 1180                 1185             


Thr Asn  Gly Lys Pro Met Pro  Ser Asp Phe Val Arg  Leu Met Asn 
    1190                 1195                 1200             


Asn His  His Val Ala Ile Phe  Gln Asp Ala Asp Gly  Asn Leu Gln 
    1205                 1210                 1215             


Glu His  Ile Val Ser Phe Tyr  Glu Ala Val Ala Arg  Val Thr Glu 
    1220                 1225                 1230             


Leu Gly  Leu Pro Val Ile Asp  Lys Glu Tyr Asn Lys  Asp Lys Gly 
    1235                 1240                 1245             


Trp Gln  Phe Leu Phe Thr Leu  Lys Gln Asn Glu Tyr  Phe Val Phe 
    1250                 1255                 1260             


Pro Asn  Pro Ala Gln Gly Phe  Asn Pro Asp Glu Ile  Asp Leu Thr 
    1265                 1270                 1275             


Asp Glu  Arg Asn Tyr Ser Arg  Ile Ser Pro Asn Leu  Tyr Arg Val 
    1280                 1285                 1290             


Gln Lys  Met Ser Ser Lys Tyr  Tyr Val Phe Arg His  His Leu Glu 
    1295                 1300                 1305             


Thr Thr  Val Glu Asp Lys Lys  Glu Leu Gln Gly Ile  Thr Trp Lys 
    1310                 1315                 1320             


Arg Ile  Gln Ser Leu Asn Asn  Leu Lys Gly Ile Val  Lys Val Arg 
    1325                 1330                 1335             


Ile Asp  His Ile Gly Arg Ile  Val Gln Ile Gly Glu  Tyr 
    1340                 1345                 1350     


<210>  918
<211>  1351
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  918

Met Phe Ser Gln Gln Asn Asn Leu Tyr Cys Glu Ser Leu Ser Lys Leu 
1               5                   10                  15      


Tyr Tyr Asn Ile Thr Lys Asn Phe Leu Ser Lys Asn Glu Val Cys Arg 
            20                  25                  30          


Ile Tyr Glu Ile Phe Phe Cys Ser Ile Leu Thr Tyr Phe Leu Lys Lys 
        35                  40                  45              


Tyr Asn His Asp Ile Phe Lys Lys Ser Ser Thr Met Lys Thr Ile Leu 
    50                  55                  60                  


Gly Leu Asp Leu Gly Val Thr Ser Ile Gly Trp Ala Val Ile Val Thr 
65                  70                  75                  80  


Asp Asp Asp Asn Thr Pro Ile Lys Ile Leu Gly Met Gly Ser Arg Ile 
                85                  90                  95      


Ile Pro Leu Ser Thr Ala Asp Lys Glu Glu Phe Gln Lys Gly Gln Ala 
            100                 105                 110         


Ile Ser Lys Asn Gln Ser Arg Thr Ile Ala Arg Thr Gln Arg Lys Gly 
        115                 120                 125             


Tyr Asn Arg Lys Gln Leu Arg Lys Glu Glu Leu Lys Lys Ile Leu Gly 
    130                 135                 140                 


Glu Leu Gly Ile Thr Pro Ser Lys Glu Leu Leu Asn Leu Pro Ser Ile 
145                 150                 155                 160 


Glu Leu Trp Lys Leu Arg Ala Asp Ala Ala Ser Gly Asn Ala Pro Leu 
                165                 170                 175     


Ser Ala Glu Gln Leu Gly Arg Ile Phe Tyr Met Leu Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Lys Ser Ala Arg Ser Glu Ala Asn Gln Asp Lys Lys Asp Thr 
        195                 200                 205             


Asp Tyr Val Ala Ala Val Lys Gly Arg His Gln Gln Leu Lys Glu Lys 
    210                 215                 220                 


Gly Gln Thr Ile Gly Gln His Phe Tyr Thr Glu Met Leu Thr Leu Glu 
225                 230                 235                 240 


Glu Asn Ser Gly His Phe Gln Val Lys Asn Asn Val Tyr Pro Arg Glu 
                245                 250                 255     


Ala Tyr Leu Glu Glu Phe Asn Ala Ile Ile Ala Ala Gln Lys Pro Gln 
            260                 265                 270         


His Ala Phe Leu Thr Asp Asp Val Val Asp Arg Leu Arg Asp Glu Val 
        275                 280                 285             


Ile Phe Tyr Gln Arg Lys Leu Lys Ser Gln Lys Gly Leu Val Ser Ile 
    290                 295                 300                 


Cys Glu Phe Glu Gly Phe Glu Arg Thr Phe Phe Asp Asn Val Lys Gln 
305                 310                 315                 320 


Lys Asp Lys Thr Ile Phe Thr Gly Pro Lys Val Ala Pro Lys Ser Ser 
                325                 330                 335     


Pro Leu Phe Gln Leu Cys Lys Ile Trp Glu Thr Val Asn Asn Ile Asn 
            340                 345                 350         


Leu Lys Val Lys Asn Thr Asp Gly Ser Lys Asn Lys Trp Ala Asp Tyr 
        355                 360                 365             


Val Pro Thr Leu Glu Gln Lys Gln Glu Ile Ile Glu His Leu Phe Lys 
    370                 375                 380                 


Asn Ala Gly Ile Ser Leu Asp Ala Leu Arg Lys Ile Leu Gly Phe Asn 
385                 390                 395                 400 


Lys Asp Glu Ile Phe Val Asn Lys Gln Ile Glu Lys Gly Leu Gln Gly 
                405                 410                 415     


Asn Thr Thr Tyr Ala Glu Ile His Lys Ile Leu Gly Asp Ser Lys Pro 
            420                 425                 430         


Glu Leu Leu Gly Phe Asn Ile Asn Ile Glu Pro Ser Lys His Ser Ala 
        435                 440                 445             


Leu Leu Val Asp Lys Arg Thr Gly Glu Ile Leu Ala Glu Lys Glu Gly 
    450                 455                 460                 


Gln Gln Val Ser Ala Glu Ile Glu Lys Glu Asn Phe Tyr Gln Leu Trp 
465                 470                 475                 480 


His Thr Ile Tyr Ser Ile Lys Asp Leu Asp Glu Cys Ala Ala Ala Leu 
                485                 490                 495     


Ile Lys Arg Phe Asn Ile Asp Pro Asn Val Ala Ala Gln Leu Ala Lys 
            500                 505                 510         


Ile Asp Phe Ser Lys Gly Ala Phe Gly Asn Lys Ser Asn Lys Ala Met 
        515                 520                 525             


Arg Lys Ile Leu Pro Tyr Leu Met Gln Gly Tyr Asn Tyr Ala Asp Ala 
    530                 535                 540                 


Cys Ser Leu Ala Gly Tyr Asn His Ser Asp Ser Gln Thr Lys Lys Glu 
545                 550                 555                 560 


Lys Glu Glu Asn Val Val Asp Asp Lys Leu Lys Leu Leu Glu Lys Asn 
                565                 570                 575     


Ser Leu Arg Gln Pro Val Val Glu Lys Ile Ile Asn Gln Met Ile His 
            580                 585                 590         


Val Val Asn Ala Ile Ile Asp Lys Tyr Gly Lys Pro Asp Glu Ile Arg 
        595                 600                 605             


Val Glu Leu Ala Arg Asp Leu Lys Lys Ser Lys Asp Glu Arg Asn Glu 
    610                 615                 620                 


Ala Glu Leu Gln Asn Ala Ser Asn Lys Lys Leu Asn Glu Glu Val Ala 
625                 630                 635                 640 


Asn Arg Leu Lys Glu Leu Gly Leu Pro Val Thr Lys Arg Phe Val Gln 
                645                 650                 655     


Lys Tyr Lys Phe Ile Phe Pro Val Arg Asp Arg Lys Ile Lys Glu Ala 
            660                 665                 670         


Gln Leu Val Asn Gln Cys Ile Tyr Cys Gly Glu Thr Phe Asn Val Thr 
        675                 680                 685             


Glu Ala Leu Ser Gly Asp Asn Phe Asp Val Asp His Ile Ile Pro Lys 
    690                 695                 700                 


Ser Met Leu Phe Asp Asp Ser Gln Thr Asn Lys Val Leu Val His Arg 
705                 710                 715                 720 


Lys Cys Asn Ala Lys Asp Lys Ser Asn Lys Thr Ala Tyr Asp Phe Ile 
                725                 730                 735     


Ala Ala Lys Gly Glu Asn Glu Leu Ala Ile Tyr Leu Asp Arg Val Asp 
            740                 745                 750         


Asp Trp Phe Lys Arg Gly Val Leu Ser Tyr Gly Lys Met Gln Arg Leu 
        755                 760                 765             


Lys Val Ser Tyr Glu Gln Tyr Ala Glu Arg Lys Gln Leu Lys Lys Glu 
    770                 775                 780                 


Thr Glu Ala Asp Ile Lys Leu Trp Glu Asn Phe Ile Glu Arg Asp Leu 
785                 790                 795                 800 


Arg Gln Thr Gln Tyr Ile Ala Lys Lys Ser Lys Asp Leu Leu Lys Lys 
                805                 810                 815     


Ile Cys Asn Asn Val Thr Val Thr Glu Gly Lys Ile Thr Ala Gln Leu 
            820                 825                 830         


Arg Asn Leu Trp Gly Trp Asp Asp Val Leu Met Asn Leu Gln Leu Pro 
        835                 840                 845             


Lys Tyr Lys Ala Leu Gly Gln Thr Glu Ile Lys Glu Trp Thr Ser Glu 
    850                 855                 860                 


His Gly Arg Arg Leu His Lys Lys Glu Glu Ile Thr Asn Trp Thr Lys 
865                 870                 875                 880 


Arg Asp Asp His Arg His His Ala Ile Asp Ala Leu Val Val Ala Cys 
                885                 890                 895     


Thr Lys Gln Gly Phe Ile Gln Arg Ile Asn Thr Leu Asn Ser Ser Asp 
            900                 905                 910         


Thr Lys Asp Ala Met Asp Arg Glu Ile Lys Ala Ala Lys Glu Lys Tyr 
        915                 920                 925             


Asn Glu Leu Tyr Asp Lys Lys Leu Leu Thr Glu Thr Asn Glu Gln Val 
    930                 935                 940                 


Lys Asn His Thr Glu Arg Phe Asn Lys Leu Glu Thr Tyr Leu Lys Ser 
945                 950                 955                 960 


Gln Gln Pro Pro Leu Phe Thr Thr Arg Tyr Val Glu Gln Glu Ala Asp 
                965                 970                 975     


Lys Ile Phe Val Ser Phe Lys Ala Gly Lys Lys Ala Ala Thr Tyr Gly 
            980                 985                 990         


Ile Arg Lys Ile Lys Lys Asp Gly  Lys Lys Val Val Val  Gln Glu Lys 
        995                 1000                 1005             


Ile Val  Val Pro Arg Gly Ala  Leu His Glu Gln Ser  Val Tyr Gly 
    1010                 1015                 1020             


Lys Ile  Lys Thr Gln Glu Lys  Asp Lys Pro Leu Lys  Tyr Leu Phe 
    1025                 1030                 1035             


Glu Asn  Pro Glu Thr Ile Val  Asp Asn Arg Ile Lys  Ile Leu Val 
    1040                 1045                 1050             


Lys Glu  Arg Leu Asp Lys Phe  Glu Gly Asp Val Lys  Lys Ala Leu 
    1055                 1060                 1065             


Thr Ser  Leu Lys Lys Glu Pro  Leu Phe Ile Asp Asn  Glu Lys Asn 
    1070                 1075                 1080             


Thr Leu  Leu Glu Lys Ala Ala  Cys Tyr Thr Asp Ala  Thr Val Leu 
    1085                 1090                 1095             


Lys Tyr  Lys Leu Gln Ala Leu  Lys Ala Asn Gln Val  Asn Asp Ile 
    1100                 1105                 1110             


Val Asp  Glu Gly Val Lys Arg  Ile Val Lys Ala Arg  Leu Ala Gln 
    1115                 1120                 1125             


His Lys  Asn Glu Lys Glu Ala  Phe Lys Asp Thr Val  Trp Phe Asn 
    1130                 1135                 1140             


Gln Glu  Lys Arg Ile Pro Ile  Val Ser Val Arg Val  Phe Ala Arg 
    1145                 1150                 1155             


Pro Asp  Ala Ser Lys Leu Ala  Ile Ile Lys Thr Asp  Glu Asn Asn 
    1160                 1165                 1170             


Lys Pro  Ile Gly Phe Ala Leu  Thr Gly Asn Asn His  His Ile Ala 
    1175                 1180                 1185             


Ile Tyr  Lys Asp Asn Thr Gly  Lys Leu Val Gln His  Ile Cys Thr 
    1190                 1195                 1200             


Phe Trp  Ser Ala Val Glu Arg  Lys Lys Phe Gly Val  Pro Thr Val 
    1205                 1210                 1215             


Val Lys  Asn Thr Gly Thr Leu  Trp Ser Glu Leu Leu  His Lys Asp 
    1220                 1225                 1230             


Leu Pro  Gly Thr Phe Leu Asp  Lys Leu Pro Gln Asp  Gly Leu Glu 
    1235                 1240                 1245             


Leu Glu  Tyr Ser Leu Gln Gln  Asn Glu Met Phe Val  Leu Gly Leu 
    1250                 1255                 1260             


Pro Lys  Glu Ser Phe Glu Asp  Ala Ile Lys Asn Asn  Asp Leu Pro 
    1265                 1270                 1275             


Leu Leu  Ser Lys His Leu Tyr  Leu Val Trp Ser Ile  Ser Asp Asn 
    1280                 1285                 1290             


Asp Tyr  Trp Phe Arg His His  Leu Glu Thr Lys Asn  Ser Glu Leu 
    1295                 1300                 1305             


Lys Ser  Thr Thr Gly Ala Lys  Glu Ser Lys Arg Tyr  Tyr Arg Phe 
    1310                 1315                 1320             


Gln Ser  Val Gly Thr Phe Thr  Lys Glu Asn Pro Ile  Lys Val Gln 
    1325                 1330                 1335             


Leu Asn  His Leu Gly Glu Ile  Thr Lys Ile Gly Glu  Pro 
    1340                 1345                 1350     


<210>  919
<211>  1352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  919

Met Lys Lys Arg Asn Arg Glu Glu Tyr Tyr Val Gly Leu Asp Ile Gly 
1               5                   10                  15      


Thr Ser Ser Val Gly Trp Ala Val Ala Thr Pro Asp Tyr Arg Ile Pro 
            20                  25                  30          


Lys Phe Arg Gly His Lys Met Trp Gly Ser Arg Leu Phe Ala Glu Gly 
        35                  40                  45              


Lys Arg Ala Ala Glu Arg Arg Gln Phe Arg Thr Asn Arg Arg Arg Leu 
    50                  55                  60                  


Ala Arg Arg Arg Asp Arg Leu Gln Ile Leu Glu Glu Leu Phe Ala Glu 
65                  70                  75                  80  


Glu Met Ala Lys Val Asp Asn Thr Phe Phe Met Arg Leu Lys Glu Ser 
                85                  90                  95      


Lys Phe Phe Leu Glu Asp Lys Thr Val Lys Glu Lys His Ile Leu Phe 
            100                 105                 110         


Ser Thr Asp Lys Phe Asp Ala Asp Arg Phe Tyr Lys Asp Asn Pro Thr 
        115                 120                 125             


Ile Tyr His Val Arg Lys Arg Leu Met Glu Glu Glu Pro Gln Asp Ile 
    130                 135                 140                 


Arg Glu Leu Phe Leu Ala Val His His Ile Leu Lys Tyr Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Ala Gly Ser Leu Glu Gln Asn Gly Ala Ser Ile Glu Glu 
                165                 170                 175     


Leu Val Arg Thr Leu Leu Gly Lys Gln Gly Leu Ala Ile Leu Asp Asp 
            180                 185                 190         


Thr Ile Asp Ser Asn Glu Met Val Asn Ile Leu Lys Asn Thr Gly Glu 
        195                 200                 205             


Thr Lys Ser Asp Arg Lys Lys Asp Val Gln Glu Leu Trp Asn Pro Leu 
    210                 215                 220                 


Lys Asp Lys Val Lys Lys Ala Gln Ala Ala Glu Met Ala Gly Leu Val 
225                 230                 235                 240 


Leu Gly Leu Lys Ser Asp Ile Ser Lys Leu Phe Ala Asn Ser Asp Tyr 
                245                 250                 255     


Lys Asp Leu Asp Ser Ser Ile Lys Asn Ile Asn Phe Ser Ser Gly Asp 
            260                 265                 270         


Tyr Glu Glu Lys Arg Asp Asp Tyr Ala Asn Ser Ile Gly Asp Asp Ile 
        275                 280                 285             


Val Leu Leu Asp Thr Met Lys Ala Leu Tyr Asp Thr Leu Ile Leu Ser 
    290                 295                 300                 


Gln Ile Leu Gln Gly Lys Arg Ser Ile Ser Ala Ala Lys Val Glu Asp 
305                 310                 315                 320 


Tyr Asn Thr His Lys Glu Gln Leu Arg Ala Leu Lys Ala Ile Leu Lys 
                325                 330                 335     


Pro His Lys Arg Leu Tyr Asn Glu Met Phe Arg Glu Asn Asn Lys Lys 
            340                 345                 350         


Asp Leu Ser Asn Tyr Val Ala Tyr Ile Ser Ser Tyr Ser Glu Gly Lys 
        355                 360                 365             


Arg Val Asn Arg Glu Gly Phe Tyr Lys Tyr Val Gln Ala Val Leu Lys 
    370                 375                 380                 


Gln Val Asp Asp Ser Asp Ser Lys Gln Ala Ile Leu Glu Ala Ile Arg 
385                 390                 395                 400 


Leu Glu Thr Phe Leu Pro Leu Leu Arg Val Lys Asp Asn Ala Val Ile 
                405                 410                 415     


Pro His Gln Ile His Gln Ala Glu Leu Glu Ala Ile Leu Glu Thr Ala 
            420                 425                 430         


Arg Lys Lys Tyr Asn Phe Leu Asn Asp Val Ser Asp Gly Val Ser Val 
        435                 440                 445             


Lys Asp Lys Ile Ile Gln Leu Phe Thr Phe Arg Ile Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Leu Asn Pro His Ser Gly Glu Thr Val Ala Asp Arg Lys Gln 
465                 470                 475                 480 


Gly Thr Gly Trp Val Val Arg Lys Gln Arg Gly Lys Val Tyr Pro Trp 
                485                 490                 495     


Asn Phe Ala Asp Met Val Asp Val Lys Ala Ser Ala Glu Ala Phe Ile 
            500                 505                 510         


Thr Asn Leu Thr Asn Lys Cys Thr Tyr Leu Ile Gly Glu Asp Val Leu 
        515                 520                 525             


Pro Lys Arg Ser Leu Leu Tyr Ser Glu Phe Met Leu Leu Asn Glu Leu 
    530                 535                 540                 


Asn Met Ile Lys Tyr Asp Gly His Glu Leu Pro Val Ala Ile Lys Gln 
545                 550                 555                 560 


Leu Tyr Ile Glu Gln Val Phe Lys Gln Glu His Lys Lys Gln Thr Lys 
                565                 570                 575     


Gly Arg Val Leu Asp Phe Leu Lys Arg Asn Gly Leu Ile Thr Gly Ser 
            580                 585                 590         


Ala Thr Ala Val Ile Thr Gly Met Asp Asp Ala Ile Lys Gly Asp Leu 
        595                 600                 605             


Gln Ser Tyr Arg Asp Met Val Arg Ile Phe Gly Ala Gly Phe Asp His 
    610                 615                 620                 


Ser Met Ala Glu Arg Ile Ile Arg Trp Val Thr Leu Phe Gly Glu Ala 
625                 630                 635                 640 


Lys Asp Ile Leu Glu Asn Lys Ile Arg Glu Val Tyr Gly Asn Ser Ile 
                645                 650                 655     


Ser Asp Ser Gln Met Arg Ala Ile Lys Lys Leu Lys Tyr Ala Asp Trp 
            660                 665                 670         


Gly Arg Leu Ser Gln Ala Phe Leu Ile Ala Val Lys Gly Cys Asp Lys 
        675                 680                 685             


Ser Gly Val Glu Arg Asn Ile Ile Thr Cys Leu Arg Ala Ser Ser Gln 
    690                 695                 700                 


Asn Leu Met Glu Ile Leu Ser Asp Gln Gly Thr Tyr Ile Lys Gln Ile 
705                 710                 715                 720 


Lys Ala Trp Asn His Gln His Thr Thr Asn Met Met Asp Thr Leu Asp 
                725                 730                 735     


Tyr Gln Leu Val Asp Asp Leu Tyr Val Ala Pro Ser Val Lys Arg Ser 
            740                 745                 750         


Ile Trp Gln Ser Leu Cys Ile Val Glu Glu Leu Val Lys Ile Met Gly 
        755                 760                 765             


Gln Ala Pro Lys Lys Ile Phe Val Glu Val Thr Arg Thr Lys Gln Ala 
    770                 775                 780                 


Glu Lys Thr Lys Lys Asp Ser Arg Lys Lys Arg Leu Val Glu Leu Tyr 
785                 790                 795                 800 


Thr Ala Ile Gly Lys Glu Gly Ala Ala Trp Lys Lys Glu Ile Glu Ala 
                805                 810                 815     


His Gln Ala Gly Asp Phe Arg Arg Lys Lys Leu Tyr Leu Tyr Tyr Thr 
            820                 825                 830         


Gln Met Gly Arg Cys Met Tyr Ser Asn Glu Pro Ile Ala Met Asp Ala 
        835                 840                 845             


Leu Phe Thr Ser Ala Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Leu 
    850                 855                 860                 


Thr Lys Asp Gly Ser Trp Asp Asn Leu Val Leu Val Lys Ser Gln Leu 
865                 870                 875                 880 


Asn Lys Glu Lys Ser Asp Gln Tyr Pro Leu Arg Pro Ala Ile Arg Glu 
                885                 890                 895     


Lys Asn Lys Ala His Trp Gln Tyr Leu Cys Arg Ala Gly Phe Ile Ser 
            900                 905                 910         


Glu Lys Lys Tyr Glu Arg Leu Met Arg Thr Thr Glu Leu Thr Ala Glu 
        915                 920                 925             


Glu Leu Ser Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Ser Gln Ala 
    930                 935                 940                 


Thr Lys Ala Val Thr Gly Leu Leu Glu Arg Ile Tyr Thr Asn Thr Glu 
945                 950                 955                 960 


Ile Cys Phe Val Lys Ala Glu Thr Val Ser Gln Phe Arg His Gln His 
                965                 970                 975     


Asn Phe Ile Lys Ile Arg Glu Leu Asn Asn Tyr His His Ala Lys Asp 
            980                 985                 990         


Ala Tyr Leu Asn Ile Val Val Gly  Asn Val Tyr His Glu  Lys Phe Thr 
        995                 1000                 1005             


Lys Asn  Pro Trp Glu Phe Ile  Ala Arg Ser Thr Glu  Arg Arg Pro 
    1010                 1015                 1020             


Tyr Asn  Leu Asn Arg Met Tyr  Asp Phe Asp Leu Leu  Lys Gln Gly 
    1025                 1030                 1035             


Arg Val  Ile Trp Gln Val Pro  Lys Ser Leu Glu Thr  Val Gln Ala 
    1040                 1045                 1050             


Met Met  Lys Ser Asn Asp Val  Arg Ile Thr Lys Lys  Leu Leu Val 
    1055                 1060                 1065             


Gln Lys  Gly Glu Leu Tyr Asp  Ala Thr Ile Thr Lys  Ala His Lys 
    1070                 1075                 1080             


Ala Lys  Glu Gly Thr Tyr Phe  Pro Leu Lys Gly Ala  Asp Ala Arg 
    1085                 1090                 1095             


Leu Arg  Asp Val His Arg Tyr  Gly Gly Tyr Glu Lys  Ile Lys Ile 
    1100                 1105                 1110             


Ala Tyr  Tyr Ser Val Phe Ser  Cys Arg Glu Lys Ala  Ser Asn Thr 
    1115                 1120                 1125             


Ile His  Thr Leu Ile Ile Pro  Ile Pro Val Tyr Lys  Ala Gly Leu 
    1130                 1135                 1140             


Leu Gln  Thr Glu Ala Asp Leu  Thr Ala Tyr Ala Trp  Gln Thr Lys 
    1145                 1150                 1155             


Gln Lys  Thr Lys Pro Ile Lys  Asp Ile Tyr Ser Ala  Ile Thr Pro 
    1160                 1165                 1170             


Ile Tyr  Lys Lys Leu Cys Ile  Asn Thr Val Val Lys  Ile Asp Gly 
    1175                 1180                 1185             


Phe Lys  Tyr Phe Leu Gly Gly  Lys Thr Gly Asp Tyr  Met Trp Leu 
    1190                 1195                 1200             


Asp Pro  Ala Ile Pro Val Val  Leu Asp Glu Gln Ala  Ala Ala Tyr 
    1205                 1210                 1215             


Leu Lys  Ala Ile Ser Lys Val  Gly Arg Ala Asp Asp  Val Glu Asn 
    1220                 1225                 1230             


Thr Ala  Gln Ala Ile Asp Leu  Ile Thr Ala Glu Ala  Asn Glu Ala 
    1235                 1240                 1245             


Phe Tyr  Asp Val Leu Leu Lys  Lys Met Glu Ala Lys  Ile Phe Ala 
    1250                 1255                 1260             


Lys Ala  Tyr Gln Thr Ile Val  Glu Lys Leu Ala Ser  Glu Thr Ala 
    1265                 1270                 1275             


Arg Glu  Arg Phe Arg Glu Leu  Ser Ile Gly Ala Gln  Cys Ser Cys 
    1280                 1285                 1290             


Leu Leu  Glu Ile Leu Asn Leu  Leu Ala Asn Asn Lys  Thr Thr Phe 
    1295                 1300                 1305             


Thr Phe  Phe Lys Asp Lys Met  Gly Val Thr Met Ser  Arg Arg Lys 
    1310                 1315                 1320             


Gln Pro  Met Asp Leu Thr Lys  Leu Glu Glu Cys Ile  Val Tyr Glu 
    1325                 1330                 1335             


Thr Ser  Ile Thr Gly Leu Tyr  Glu Lys Ala Val Ser  Leu Leu 
    1340                 1345                 1350         


<210>  920
<211>  1352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  920

Met Gly Lys Arg Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Trp Ala Val Met Asp Asp Asp Phe Lys Ile Met Arg Val Lys Gly 
            20                  25                  30          


Lys Lys Gly Ile Gly Val Arg Leu Phe Glu Glu Gly Lys Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Gly His Arg Thr Thr Arg Arg Arg Tyr Ser Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Asn Leu Leu Glu Glu Ile Phe Asp Pro Glu Ile Ala Lys 
65                  70                  75                  80  


Val Asp Pro Thr Phe Phe Ala Arg Leu Lys Glu Ser Asn Leu Ser Pro 
                85                  90                  95      


Lys Asp Asp Arg Lys Gln Tyr Ser Gly Asn Leu Ile Phe Pro Glu Lys 
            100                 105                 110         


Ala Lys Glu Glu Ile Arg Glu Trp Thr Glu Lys Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Asn Ala Leu Met Thr Glu Asn Arg Gln Phe Asp Ile Arg 
    130                 135                 140                 


Glu Val Phe Leu Ala Leu His His Ile Val Lys Tyr Arg Gly Asn Phe 
145                 150                 155                 160 


Leu Phe Asn Gly Asn Phe Gln Thr Gly Glu Val Asn Leu Ser Asp Arg 
                165                 170                 175     


Phe Asp Asp Ile Asn Ser Ala Phe Ile Ser Ala Cys Gly Glu Glu Glu 
            180                 185                 190         


Ala Pro Gln Ile Pro Ser Glu Leu Tyr Asn Asp Ile Gln Glu Leu Leu 
        195                 200                 205             


Phe Asp Lys Asn Thr Arg Lys Leu Asp Asn Gln Lys Gln Leu Ala Thr 
    210                 215                 220                 


Lys Leu Ser Gln Asp Asn Asp Lys Asp Trp Ser Thr Gln Phe Ser Lys 
225                 230                 235                 240 


Ala Leu Phe Gly Tyr Lys Thr Lys Phe Lys Val Val Leu Gln Ser Asn 
                245                 250                 255     


Glu Asp Leu Asp Asp Phe Gln Leu Asp Ser Asp Asp Ala Asp Asp Lys 
            260                 265                 270         


Ile Ala Ser Ile Ser Glu Gln Ile Asp Asp Asp Arg Gly Ser Ile Leu 
        275                 280                 285             


Asp Asn Ile Lys Ile Leu Tyr Ser Arg Ile Thr Leu Asn Glu Ile Val 
    290                 295                 300                 


Pro Asp Gly Lys Thr Leu Ser Gln Ser Met Ile Glu Lys Tyr Asn Leu 
305                 310                 315                 320 


His Arg Asp His Leu Lys Glu Ile Lys Asp Gln Leu Lys His Thr Asp 
                325                 330                 335     


Asn Trp Val Lys Phe Asp Asp Leu Tyr Ser Gln Tyr Val Asn Gly Lys 
            340                 345                 350         


Leu Glu Ser Asp Arg His Lys Lys Leu Ser Ser Asp Asp Phe Tyr Lys 
        355                 360                 365             


Lys Val Leu Thr Leu Phe Asp Gly Glu Leu Lys Thr Arg Val Lys Glu 
    370                 375                 380                 


Trp Gln Glu Lys Gly Glu Phe Leu Pro Lys Gln Arg Thr Gly Ser Asn 
385                 390                 395                 400 


Gly Val Ile Pro His Gln Leu His Gln Asn Glu Leu Asp Gln Ile Ile 
                405                 410                 415     


Glu Asn Gln Ser Lys Tyr Tyr Pro Trp Leu Ala Thr Leu Asn Pro Asn 
            420                 425                 430         


Glu Lys Arg Gln Arg Val Ala Lys Tyr Lys Leu Ser Glu Leu Val Ala 
        435                 440                 445             


Phe Arg Ile Pro Tyr Tyr Val Gly Pro Met Val Thr Lys Asp Gln Ser 
    450                 455                 460                 


Asp Phe Ala Trp Met Val Arg Lys Ser Asp Glu Lys Glu Ile Thr Pro 
465                 470                 475                 480 


Trp Asn Phe Asp Gln Val Val Asp Arg Asp Lys Ser Ala Glu Glu Phe 
                485                 490                 495     


Ile Asn Arg Met Thr Thr Lys Asp Thr Tyr Leu Ile Gly Glu Asp Val 
            500                 505                 510         


Leu Pro Ala Lys Ser Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu 
        515                 520                 525             


Leu Asn Asn Ile Arg Ile Asn Gly Lys Pro Leu Thr Thr Asp Glu Lys 
    530                 535                 540                 


His Gln Val Tyr Arg Asp Leu Phe Gln Asn Ala Lys Ser Ile Ser Thr 
545                 550                 555                 560 


Lys Lys Phe His Asn Tyr Leu Val Cys Lys Leu Asn Lys Pro Asn His 
                565                 570                 575     


Leu Asn Ile Thr Gly Phe Ser Asp Leu Thr Lys Phe Asn Ser Ser Leu 
            580                 585                 590         


Asp Ser Tyr Ile Asp Leu Lys Lys Ile Phe Ala Asp Gln Ile Asp Asp 
        595                 600                 605             


Gln Ser Leu Gln Asp Asp Phe Glu Lys Met Ile Arg Trp Ser Thr Val 
    610                 615                 620                 


Phe Glu Asp Lys Gln Ile Phe Glu Arg Lys Leu Ser Glu Ile Asn Trp 
625                 630                 635                 640 


Leu Thr Asp Gln Glu Arg Ser Glu Val Ala Lys Leu Arg Tyr Arg Gly 
                645                 650                 655     


Trp Gly Arg Leu Ser Lys Arg Leu Leu Ala Gly Ile His Asp Glu Asn 
            660                 665                 670         


Gly Gln Arg Ile Ile Asp Ala Leu Trp Asn Ser Pro Lys Asn Phe Met 
        675                 680                 685             


Gln Ile Gln Ala Glu Val Ala Phe Asn Lys Gln Ile Asn Asp Leu Asn 
    690                 695                 700                 


Thr Lys Glu Leu Ser Asp Asn Asn Val Glu Asp Val Leu Ala Asp Ala 
705                 710                 715                 720 


Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Leu Leu Val 
                725                 730                 735     


Val Glu Asp Ile Gln Lys Ala Met His Gly Asp Ala Pro Thr Ser Ile 
            740                 745                 750         


Ser Leu Glu Phe Ala Arg Glu Glu Gln Glu Ser Ser Arg Thr Arg Ser 
        755                 760                 765             


Arg Arg Ala Ala Leu Ser Ala Thr Tyr Arg Gln Ile Ser Asp Glu Ile 
    770                 775                 780                 


Val Ser Ala Thr Val Lys Gly Glu Leu Gly Lys Ala Thr Ser Ile Ser 
785                 790                 795                 800 


Asp Arg Leu Tyr Leu Tyr Phe Thr Gln Gly Gly Arg Asp Met Tyr Thr 
                805                 810                 815     


Gly Glu Pro Ile Asn Ile Asp Glu Ile Ser Arg Gly Tyr Glu Ile Asp 
            820                 825                 830         


His Ile Leu Pro Gln Ser Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg 
        835                 840                 845             


Val Leu Val Lys Gly Ala Ile Asn Asn Gly Lys Ser Ala Asn Tyr Val 
    850                 855                 860                 


Met Asp Gln Ser Trp Ala Pro Asp Arg Leu Ala Trp Trp Lys Lys Leu 
865                 870                 875                 880 


Leu Asp Asn Arg Leu Ile Ser Arg Lys Lys Tyr Gln Ser Leu Thr Leu 
                885                 890                 895     


Thr Pro Gly Lys Ile Asn Lys Tyr Ser Gln Asn Gly Phe Ile Asn Arg 
            900                 905                 910         


Gln Leu Val Glu Thr Arg Gln Val Ile Lys Leu Val Ala Asn Ile Leu 
        915                 920                 925             


Asn Asp Gln Tyr Ser Asn Asp Asp Thr Lys Ile Ile Val Val Lys Ala 
    930                 935                 940                 


Ala Leu Asn Ser Gln Leu Arg Gly Phe Leu Glu Leu Phe Lys Asn Arg 
945                 950                 955                 960 


Asn Ile Asn Asp Tyr His His Gly Met Asp Ala Tyr Leu Ser Thr Phe 
                965                 970                 975     


Ile Gly Asn Tyr Leu Tyr Lys Lys Tyr Pro Lys Leu Arg Ser Tyr Phe 
            980                 985                 990         


Val Tyr Gly Glu Phe Lys Lys Phe  Ser Gly Lys Gln Asn  Leu Lys Ser 
        995                 1000                 1005             


Phe Asn  Phe Leu His Asp Leu  Glu Asp Asp Ser Ile  Asn Ile Ile 
    1010                 1015                 1020             


Ser Asn  Asp Asp Gly Glu Ile  Val Gly Asn His His  Glu Met Ile 
    1025                 1030                 1035             


Glu Glu  Leu Glu Lys Ala Tyr  Gln Tyr Lys Tyr Met  Leu Val Ser 
    1040                 1045                 1050             


Arg Glu  Val Phe Glu Lys Thr  Gly Asp Leu Phe Asn  Val Thr Val 
    1055                 1060                 1065             


Tyr Ser  Arg Lys Ala Lys Lys  Thr Leu Ile Pro Ile  Lys Gln Asp 
    1070                 1075                 1080             


Lys Pro  Ile Asn Ile Tyr Gly  Gly Tyr Ser Gly Asn  Asn Asp Ala 
    1085                 1090                 1095             


Tyr Met  Ala Ile Val Lys Leu  Pro Gly Lys Lys Gly  Lys Pro Asp 
    1100                 1105                 1110             


Thr Tyr  Lys Ile Val Gly Ile  Pro Leu Arg Phe Ala  Gly Glu Leu 
    1115                 1120                 1125             


Glu Arg  Ala Lys Gln Leu Gly  Glu Ser Ala Tyr Arg  Glu Lys Leu 
    1130                 1135                 1140             


Ser Ala  Val Ile Thr Pro Gln  Phe Thr Lys Glu Lys  Lys Asp Arg 
    1145                 1150                 1155             


Lys Thr  Gly Glu Ile Thr Lys  Val Val Asp Asp Phe  Glu Val Val 
    1160                 1165                 1170             


Leu Asp  Arg Val Pro Tyr Arg  Gln Leu Phe Lys Asp  Ser Asp Lys 
    1175                 1180                 1185             


Trp Phe  Met Leu Gly Ser Ala  Ala Tyr Gln Tyr Asn  Ala Lys Gln 
    1190                 1195                 1200             


Leu Val  Leu Asn Asn Asp Asp  Val Lys Leu Leu Asn  Glu Gln Gly 
    1205                 1210                 1215             


Arg Lys  Lys Leu Glu Leu Pro  Asn Glu Lys Leu Ser  Ser Ala Leu 
    1220                 1225                 1230             


Asn Asp  Val Phe Asp His Ile  Ile Gln Ile Met Asn  Ser Lys Phe 
    1235                 1240                 1245             


Gly Leu  Tyr Asp Lys Asn Gly  Phe Arg Thr Lys Leu  Thr Glu Ala 
    1250                 1255                 1260             


Ala Ser  Ile Phe Tyr Asp Leu  Pro Leu Arg Ala Glu  Tyr Asn Gly 
    1265                 1270                 1275             


Asn Lys  Lys Val Thr Asp Gly  Lys Leu Glu Val Leu  Asp Asn Ile 
    1280                 1285                 1290             


Leu Ile  Gly Leu His Ala Asp  Pro Ser Phe Gly Asn  Leu Lys Gln 
    1295                 1300                 1305             


Leu Asn  Met Ser Thr Pro Phe  Gly Gln Met Gln Thr  Lys Gly Gly 
    1310                 1315                 1320             


Val Val  Leu Asn Gly Asn Ser  Thr Leu Ile Tyr Gln  Ser Pro Thr 
    1325                 1330                 1335             


Gly Leu  Phe Glu Arg Arg Val  Lys Leu Lys Asp Leu  Ser Lys 
    1340                 1345                 1350         


<210>  921
<211>  1352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  921

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ile Asn Glu Gly Glu Asn Asp Asn Glu Thr Ser Ser Ile Ile 
            20                  25                  30          


Asn Ala Gly Val Arg Ile Val Pro Leu Thr Thr Asp Glu Glu Ala Asp 
        35                  40                  45              


Phe Lys Lys Gly Asn Thr Ile Ser Ile Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Gly Leu Gln Arg Phe Lys Gln Arg Arg Asp Ala 
65                  70                  75                  80  


Leu Leu Gln Ser Phe Arg Gly Ile Asn Phe Ile Ser Ser Asn Tyr Val 
                85                  90                  95      


Tyr Ala Glu Thr Gly Lys Gln Thr Thr Phe Ser Ser Tyr Lys Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Thr Glu Leu Val Thr Lys Glu Glu Leu Val Gln Val 
        115                 120                 125             


Leu Leu Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Thr Ala Glu Glu Gly Asp Ala Ile Asp Gly Met Lys Ile Ala Lys 
145                 150                 155                 160 


Glu Ile Phe Glu Asn Asn Leu Thr Pro Gly Gln Trp Val Tyr Asn Ser 
                165                 170                 175     


Leu Ser Ile Gly Gly Lys Phe Ile Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Lys Glu Val Glu Lys Ile Val Arg Phe Gln Asn Gln Phe His Pro 
        195                 200                 205             


Glu Gln Met Asn Glu Lys Leu Leu Glu Asn Ile Gln Gly Lys Ala Arg 
    210                 215                 220                 


Thr Val Thr Ser Gln Tyr Phe Ser Arg Ile Leu Asn Ile Pro Leu Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Thr Arg Glu Glu Thr Lys Leu Gln His Tyr Lys Trp 
                245                 250                 255     


Arg Asn Glu Ala Leu Thr Glu Glu Leu Asp Leu Lys Val Leu Ala Phe 
            260                 265                 270         


Ile Ile Thr Glu Ile Asn Asn Gln Ile Asn Gln Ser Asn Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Gln Glu Thr 
    290                 295                 300                 


Val Gly Gln Phe Gln Tyr Asn Gln Leu Lys Glu Asn Ala His Thr Lys 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Lys Ile Trp Glu Thr Gln Ser Lys Phe Tyr Pro Glu Leu Asn Asp Glu 
            340                 345                 350         


Leu Lys Ser Glu Val Arg Asp Ile Thr Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys His Leu Val Ser Phe Cys Glu Phe Glu Lys Gly His 
    370                 375                 380                 


Lys Ala Ile Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Arg Ile Trp 
385                 390                 395                 400 


Gln Asn Ile Asn Asn Ile Val Val Lys Asn Glu Thr Thr Lys Glu Pro 
                405                 410                 415     


Phe Leu Leu Gly Glu Asp Leu Lys Lys Val Ile Ala Gln Glu Leu Ser 
            420                 425                 430         


Phe Lys Glu Ser Met Thr Asp Ser Gln Leu Leu Thr Phe Cys Asp Leu 
        435                 440                 445             


Lys Lys Gly Thr His Thr Val Asn Phe Lys Lys Ile Glu Gly Asn Arg 
    450                 455                 460                 


Thr Asn Thr Ser Leu Phe Lys Ala Phe Glu Lys Ile Leu Val Leu Glu 
465                 470                 475                 480 


Gly Tyr Asp Glu Leu Asp Leu Ser Lys Leu Asp Ala Ser Asp Ile Lys 
                485                 490                 495     


Ser Ile Ile Lys Ser Ala Phe Ser Asp Leu Glu Ile Ser Thr Ala Ile 
            500                 505                 510         


Leu Asp Phe Asp Pro Thr Ile Gln Gly Asn Asp Tyr Asp Lys Gln Ser 
        515                 520                 525             


Tyr Tyr Gln Leu Trp His Leu Leu Tyr Ser Ser Glu Glu Asp Asp Phe 
    530                 535                 540                 


Leu Lys Lys Ser Leu Met Asp Lys Phe Gly Phe Lys Glu Ser His Leu 
545                 550                 555                 560 


Pro Phe Leu Leu Asn Ile Asn Leu Gln Ala Asp Tyr Gly Ser Leu Ser 
                565                 570                 575     


Ala Lys Ala Ile Lys Lys Val Leu Pro His Leu Met Asp Gly His Ile 
            580                 585                 590         


Tyr Asp Lys Ala Cys Thr Leu Val Gly Tyr Asn His Ser Ser Ser Leu 
        595                 600                 605             


Thr Thr Asp Gln Asn Asn Glu Arg Val Leu Lys Asp Thr Leu Glu Leu 
    610                 615                 620                 


Leu Lys Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn 
625                 630                 635                 640 


Gln Met Ile Asn Val Ile Asn Ala Ile Ile Lys Asp Pro Ala Met Gly 
                645                 650                 655     


Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Asn Asn 
            660                 665                 670         


Asn Glu Gln Arg Ser Glu Met Thr Lys Ala Ile Asn Lys Ser Thr Thr 
        675                 680                 685             


Glu His Glu Gln Ile Arg Lys Leu Leu His Thr Glu Phe Gly Ile His 
    690                 695                 700                 


Arg Val Thr Arg Asn Asp Ile Ile Arg Tyr Lys Leu Trp Lys Glu Cys 
705                 710                 715                 720 


Asp Gly Ile Ser Leu Tyr Thr Gly Lys Pro Ile Glu Pro Ser Lys Leu 
                725                 730                 735     


Phe Thr Lys Asp Tyr Asp Ile Glu His Ile Ile Pro Lys Ser Arg Leu 
            740                 745                 750         


Phe Asp Asp Ser Phe Ser Asn Lys Thr Ile Cys Glu Arg Gln Leu Asn 
        755                 760                 765             


Ile Asp Lys Ser Asn Lys Thr Ala Tyr Ser Phe Phe Gln Glu Lys Leu 
    770                 775                 780                 


Ser Ala Glu Glu Phe Asp Gln Phe Glu Lys Arg Val Lys Ser Leu Phe 
785                 790                 795                 800 


Gly Lys Ile Ser Arg Thr Lys Gln Asn Lys Leu Leu Met Ala Asn Asn 
                805                 810                 815     


Glu Ile Pro Glu Gly Phe Ile Asp Arg Gln Leu Arg Glu Thr Gln Tyr 
            820                 825                 830         


Ile Ala Lys Lys Ala Lys Glu Ile Leu Leu Glu Val Ser Arg Asn Val 
        835                 840                 845             


Thr Ala Thr Ile Gly Ser Val Thr Asp Lys Leu Arg Asp Asp Trp Glu 
    850                 855                 860                 


Leu Val Asp Val Met Lys Glu Leu Asn Trp Glu Lys Tyr Asp Lys Leu 
865                 870                 875                 880 


Gly Leu Thr Tyr Ile Glu Glu Gly Lys Asn Gly Glu Arg Leu Tyr Lys 
                885                 890                 895     


Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp 
            900                 905                 910         


Ala Ile Thr Val Ala Phe Thr Lys Pro Ala Tyr Ile Gln Tyr Leu Asn 
        915                 920                 925             


Asn Leu Asn Ala Arg Thr Asn Thr Asp Lys Lys Gly Asn Ser Ile Ile 
    930                 935                 940                 


Gly Ile Glu Asn Lys Tyr Leu Lys Arg Asp Lys Asn Asn Lys Leu Arg 
945                 950                 955                 960 


Phe Ile Glu Pro Ile Pro Asn Phe Arg Glu Val Ala Lys Lys Gln Leu 
                965                 970                 975     


Glu Ser Ile Leu Ile Ser Tyr Lys Ala Lys Asn Lys Val Val Thr Lys 
            980                 985                 990         


Asn Lys Asn Val Thr Lys Lys Ser  Asn Gly Thr Asn Gln  Lys Ile Gln 
        995                 1000                 1005             


Leu Thr  Pro Arg Gly Gln Leu  His Lys Glu Thr Val  Tyr Gly Lys 
    1010                 1015                 1020             


Leu Gln  Gln Tyr Val Thr Lys  Glu Glu Lys Ile Asn  Ala Asn Phe 
    1025                 1030                 1035             


Thr Pro  Glu Tyr Val Thr Lys  Val Ala Lys Lys Glu  Tyr Arg Glu 
    1040                 1045                 1050             


Ala Leu  Leu Lys Arg Leu Ile  Glu Asn Glu Asn Asp  Pro Lys Lys 
    1055                 1060                 1065             


Ala Phe  Thr Gly Lys Asn Ala  Leu Ser Lys Asn Pro  Ile Tyr Ile 
    1070                 1075                 1080             


Ser Leu  Glu Asn Asn Ile Leu  Val Pro Glu Lys Val  Lys Ile Val 
    1085                 1090                 1095             


Trp Leu  Lys Ser Asp Tyr Thr  Ile Arg Lys Asp Ile  Thr Pro Asp 
    1100                 1105                 1110             


Leu Lys  Ile Asp Lys Val Ile  Asp Ile Gly Leu Lys  Thr Ile Leu 
    1115                 1120                 1125             


Gln Asn  Arg Leu Asn Glu Phe  Asn Gly Asp Thr Lys  Lys Ala Phe 
    1130                 1135                 1140             


Val Asn  Leu Asp Glu Asn Pro  Ile Trp Gln Asn Lys  Glu Lys Gly 
    1145                 1150                 1155             


Ile Ala  Ile Lys Arg Val Thr  Ile Ser Gly Val Ser  Asn Ala Gln 
    1160                 1165                 1170             


Ala Leu  His Thr Lys Lys Asp  His Leu Gly Asn Glu  Ile Leu Asp 
    1175                 1180                 1185             


Glu Asn  Gly Gln Pro Leu Pro  Val Asp Phe Val Ser  Thr Gly Asn 
    1190                 1195                 1200             


Asn His  His Val Ala Ile Tyr  Arg Asp Glu Lys Gly  Asn Leu Gln 
    1205                 1210                 1215             


Glu Glu  Val Val Ser Phe Phe  Glu Ala Val Thr Arg  Asn Asn Leu 
    1220                 1225                 1230             


Gly Leu  Ser Ile Ile Asn Lys  Asn His Glu Lys Gly  Trp Glu Phe 
    1235                 1240                 1245             


Leu Phe  Ser Met Lys Gln Asn  Glu Phe Phe Ile Phe  Pro Thr Asp 
    1250                 1255                 1260             


Gly Phe  Asn Pro His Glu Ile  Asp Leu Leu Asn Thr  Glu Asn Tyr 
    1265                 1270                 1275             


His Leu  Ile Ser Arg Asn Met  Phe Arg Val Gln Lys  Ile Ala Thr 
    1280                 1285                 1290             


Lys Asn  Tyr Met Phe Asn His  His Leu Glu Thr Lys  Ser Val Asp 
    1295                 1300                 1305             


Gly Glu  Met Leu Lys Thr Lys  Lys Gln Leu Ser Lys  Ile Ser Tyr 
    1310                 1315                 1320             


His Phe  Ile Gln Thr Pro Ser  Tyr Leu Asn Tyr Ile  Ile Lys Ile 
    1325                 1330                 1335             


Arg Ile  Asn His Leu Gly Gln  Ile Val Gln Leu Gly  Glu Tyr 
    1340                 1345                 1350         


<210>  922
<211>  1352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  922

Met Lys Gln Asp Tyr Tyr Ile Gly Leu Asp Met Gly Thr Glu Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Val Asp Tyr Asn Leu Ile Lys Lys Arg Gly 
            20                  25                  30          


Gln Asp Tyr Trp Gly Val Tyr Leu Phe Asp Glu Ala Gln Thr Ala Gln 
        35                  40                  45              


Gly Arg Arg Ala Phe Arg Thr Asn Arg Arg Arg Leu Ala Arg Val Arg 
    50                  55                  60                  


His Arg Leu Asn Leu Leu Gln Glu Leu Phe Asp Ser Glu Ile Ser Ala 
65                  70                  75                  80  


Lys Asp Phe Gly Phe Phe Tyr Arg Leu Glu Asn Ser Lys Tyr Phe Asp 
                85                  90                  95      


Glu Asp Lys Pro Glu Ile Ala Arg Gly Lys Phe Leu Leu Phe Asn Asp 
            100                 105                 110         


Lys Asp Phe Gly Asp Lys Glu Tyr Tyr Lys Asn Tyr Pro Thr Ile Phe 
        115                 120                 125             


His Leu Arg Asn Ala Leu Arg Thr Gln Glu Ile Lys Asp Val Arg Leu 
    130                 135                 140                 


Leu Tyr Leu Ala Val His His Ile Leu Lys Asn Arg Gly His Phe Leu 
145                 150                 155                 160 


Phe Gly Glu Gln Asp Phe Asn Val Asn Asp Ala Ser Asp Val Ser Gly 
                165                 170                 175     


Glu Phe Glu Lys Ile Asn Arg Phe Leu Ser Glu Asn Asp Gly Ala Gly 
            180                 185                 190         


Phe Asp Arg Glu Lys Ile Pro Glu Ile Phe Glu Thr Leu Cys Asn Glu 
        195                 200                 205             


Asn Leu Ala Lys Arg Glu Arg Lys Glu Lys Ile Leu Glu Leu Ala Gly 
    210                 215                 220                 


Asn Ser Lys Asn Lys Leu Leu Gly Glu Ala Val Asn Ala Val Val Gly 
225                 230                 235                 240 


Leu Asn Phe Ser Leu Ser Lys Met Phe Gly Glu Asp Arg Asp Phe Gln 
                245                 250                 255     


Gly Glu Asn Lys Met Ser Phe Thr Asp Ser Ser Phe Glu Glu Lys Leu 
            260                 265                 270         


Ala Lys Ala Arg Met Ala Leu Asp Asp Asp Glu Ala Asp Phe Leu Glu 
        275                 280                 285             


Thr Leu Lys Lys Val Gln Ser Val Ala Ser Leu His Asn Ile Leu His 
    290                 295                 300                 


Gly Lys Glu Phe Ile Ser Glu Ala Lys Thr Glu Ala Tyr Glu Thr His 
305                 310                 315                 320 


Arg Arg Asp Leu Ser Glu Leu Lys Lys Tyr Val Arg Glu Asn Cys Pro 
                325                 330                 335     


Glu Lys Tyr Ala Leu Val Phe Arg Arg Gln Val Lys Lys Gly Lys Lys 
            340                 345                 350         


Asn Thr Ser Gly Ala Asp Glu Asn Glu Lys Asp Asp Lys Lys Val Asn 
        355                 360                 365             


Asn Tyr Ala Ala Tyr Ile Gly Met Asp Lys Gly Lys Gly Phe Glu Lys 
    370                 375                 380                 


Cys Lys Lys Glu Asp Phe Tyr Ala Phe Leu Lys Lys Glu Ile Gly Ile 
385                 390                 395                 400 


Asn Asp Glu Ser Leu Leu Ala Lys Ile Asp Glu Gly Ile Phe Leu Pro 
                405                 410                 415     


Lys Gln Ile Ser Ala Glu Asn Cys Asp Ile Pro Tyr Gln Val His Leu 
            420                 425                 430         


Ala Glu Leu Lys Ala Ile Leu Ala Asn Ala Glu Asn Tyr Phe Pro Phe 
        435                 440                 445             


Leu Lys Gln Lys Asp Glu Asn Cys Glu Met Thr Val Ser Glu Lys Ile 
    450                 455                 460                 


Ile Ser Leu Met Lys Phe Arg Val Pro Tyr Tyr Val Gly Pro Phe Thr 
465                 470                 475                 480 


Asp Lys Glu Thr Arg Phe Ser Trp Met Ile Lys Lys Glu Gly Leu Ser 
                485                 490                 495     


Thr Gln Lys Ile Thr Pro Trp Asn Phe Asp Asp Val Val Asp Arg Asp 
            500                 505                 510         


Ala Ser Glu Lys Lys Phe Ile Glu Arg Met Thr Asn Phe Cys Thr Tyr 
        515                 520                 525             


Leu Thr Gly Glu Lys Val Leu Pro Ala Ser Ser Phe Leu Tyr Ser Glu 
    530                 535                 540                 


Tyr Ala Phe Leu Asn Glu Leu Asn Asn Leu Lys Val Asn Gly Thr Lys 
545                 550                 555                 560 


Asp Leu Arg Val Lys Asn Ile Leu Phe Asp Phe Ala Lys Thr Asn Lys 
                565                 570                 575     


Lys Leu Thr Leu Lys Lys Cys Cys Glu Gln Leu Val Lys Asn Gly Leu 
            580                 585                 590         


Leu Pro Glu Gly Ser Lys Ala Arg Asp Val Phe Ser Gly Thr Asp Gly 
        595                 600                 605             


Asp Phe Lys Asn Ser Leu Ser Ser Tyr Ile Asp Phe Lys Arg Ile Ile 
    610                 615                 620                 


Gly Asp Lys Ala Asp Leu Tyr Arg Asp Met Cys Glu Lys Ile Ile Leu 
625                 630                 635                 640 


Trp Ile Thr Val Ile Ser Asp Lys Glu Arg Leu Val Lys Ile Ile Lys 
                645                 650                 655     


Asp Lys Tyr Gly Thr Val Leu Ser Asp Glu Glu Ile Lys Gly Leu Lys 
            660                 665                 670         


Ala Leu Asn Tyr Thr Lys Trp Gly Arg Leu Ser Glu Lys Leu Leu Asp 
        675                 680                 685             


Gly Ile Phe Glu Thr Asp Ser Glu Gly Glu Val Gly Asn Val Ser Ile 
    690                 695                 700                 


Ile Glu Lys Met Arg Thr Asp Thr Glu Asn Phe Met Glu Leu Leu Ser 
705                 710                 715                 720 


Asn Lys His Asn Tyr Lys Arg Ala Ile Glu Leu Ala Asn Ser Glu Ser 
                725                 730                 735     


Ile Val Asp Glu Arg Val Thr Tyr Arg Ala Val Glu Glu Leu Tyr Cys 
            740                 745                 750         


Ser Pro Ala Val Lys Arg Ser Ile Trp Ser Ala Leu Lys Ile Val Arg 
        755                 760                 765             


Glu Ile Val Lys Val Gln Gly His Ser Pro Lys Lys Ile Phe Val Glu 
    770                 775                 780                 


Thr Thr Arg Glu Lys Pro Asp Val Thr Arg Lys Gly Gln Arg Thr Val 
785                 790                 795                 800 


Ser Arg Lys Gln Gln Leu Leu Asp Lys Tyr Arg Glu Asn Leu Arg Asn 
                805                 810                 815     


Glu Thr Glu Leu Ile Lys Lys Ile Glu Glu Thr Glu Asp Ser Lys Phe 
            820                 825                 830         


Asn Ser Asp Lys Leu Phe Phe Tyr Phe Thr Gln Met Gly Lys Asp Ala 
        835                 840                 845             


Tyr Thr Gly Glu Lys Ile Glu Phe Asp Asp Val Phe Lys Thr Glu Lys 
    850                 855                 860                 


Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Lys Ile Lys Asp Asp Ser 
865                 870                 875                 880 


Leu Asp Asn Arg Val Leu Val Arg Arg Lys Ile Asn Gln Ser Val Lys 
                885                 890                 895     


Lys Asp Ser Tyr Pro Leu Ser Ala Glu Ile Arg Asn Arg Met Glu Gly 
            900                 905                 910         


Leu Trp Arg Gln Leu Arg Asp Lys Lys Leu Ile Ser Asp Glu Lys Phe 
        915                 920                 925             


Tyr Arg Leu Thr Arg Ser Thr Pro Leu Thr Glu Lys Glu Cys Gly Asp 
    930                 935                 940                 


Phe Ile Asn Ala Gln Leu Ile Ala Thr Ser Gln Ser Ala Lys Ala Ala 
945                 950                 955                 960 


Ile Gln Leu Leu Lys Arg Met Leu Pro Glu Ser Glu Ile Val Tyr Ser 
                965                 970                 975     


Lys Ala Gly Asn Val Asn Glu Phe Lys Lys Arg Leu Glu Phe Val Lys 
            980                 985                 990         


Ile Arg Glu Leu Asn Asp Leu His  His Ala Lys Asp Ala  Tyr Phe Asn 
        995                 1000                 1005             


Ile Val  Val Gly Asn Val Phe  Asn Thr Lys Phe Asn  His Asn Pro 
    1010                 1015                 1020             


Ala Val  Phe Gln Arg Ser Asn  Gly Thr Gly Asn Tyr  Asp Phe Glu 
    1025                 1030                 1035             


Arg Leu  Tyr Asp Phe Asp Ile  Thr Asn Ala Trp Lys  Lys Gly Asp 
    1040                 1045                 1050             


Glu Asn  Arg Ile Arg Ser Val  Ala Glu Lys Asn Thr  Cys Arg Val 
    1055                 1060                 1065             


Val Arg  Phe Ser Lys Thr Glu  Lys Gly Gln Phe Tyr  Asp Ala Thr 
    1070                 1075                 1080             


Ile Tyr  Pro Ala Gly Lys Asn  Asp Lys Leu Ala Pro  Val Lys Gly 
    1085                 1090                 1095             


Lys Ala  Pro Leu Asn Asp Thr  Ser Lys Tyr Gly Gly  Tyr Asp Lys 
    1100                 1105                 1110             


Glu Thr  Thr Ala Tyr Phe Met  Leu Val Lys Ser Arg  Asp Lys Lys 
    1115                 1120                 1125             


Lys Lys  Asp Lys Thr Leu Leu  Ser Leu Glu Cys Val  Thr Val Leu 
    1130                 1135                 1140             


Ala Asp  Lys Asn Phe Arg Thr  Ala Glu Asp Lys Leu  Lys Tyr Cys 
    1145                 1150                 1155             


Glu Lys  Glu Leu Gly Leu Lys  Glu Pro Glu Ile Leu  Ile Glu Lys 
    1160                 1165                 1170             


Ile Lys  Leu Asn Thr Leu Phe  Lys Ile Asn Gly Ser  Tyr Ala Trp 
    1175                 1180                 1185             


Ile Arg  Gly Arg Thr Asn Asp  Arg Leu Val Leu Cys  Asn Ala Asn 
    1190                 1195                 1200             


Gln Leu  Trp Leu Asp Asn Gly  Ser Ala Lys Glu Leu  Lys Lys Ile 
    1205                 1210                 1215             


Ser Asn  His Phe Arg Asp Val  Lys Lys Tyr Asn Asn  Lys Asp Leu 
    1220                 1225                 1230             


Pro Val  Arg Glu Glu Glu Phe  Ser Lys Glu Gln Asn  Leu Ala Leu 
    1235                 1240                 1245             


Tyr Asp  Leu Phe Thr Glu Lys  Leu Gly Gly Lys Ile  Tyr Ala Gly 
    1250                 1255                 1260             


Leu Pro  Val Gln Gly Gln Ile  Lys Asn Leu Glu Lys  Gly Arg Asp 
    1265                 1270                 1275             


Lys Phe  Glu Ala Leu Glu Leu  Lys Asp Gln Leu Phe  Val Leu Met 
    1280                 1285                 1290             


Glu Ile  Leu Lys Phe Met Gln  Cys Asn Ser Met Thr  Ser Asn Ile 
    1295                 1300                 1305             


Lys Leu  Ile Gly Gly Ala Glu  Asn Ala Gly Lys Asn  Arg Ile Ser 
    1310                 1315                 1320             


Lys Leu  Ile Gln Asp Ser Asp  Val Lys Met Ile Phe  Cys Ser Pro 
    1325                 1330                 1335             


Thr Gly  Tyr Phe Cys Lys Val  Val Asp Phe Lys Arg  Phe Leu 
    1340                 1345                 1350         


<210>  923
<211>  1352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  923

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ile Lys Glu Pro Glu Lys Asp Val Val Gly Ser Glu Ile Val 
            20                  25                  30          


Asp Met Gly Val Arg Ile Val Pro Leu Ser Ser Asp Glu Glu Asn Asp 
        35                  40                  45              


Phe Ala Lys Gly Asn Thr Ile Ser Ile Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Asn Leu Gln Arg Phe Lys Gln Arg Arg Asn Ala 
65                  70                  75                  80  


Leu Leu Glu Ile Phe Lys Glu Lys Lys Leu Ile Ser Thr Asn Phe Lys 
                85                  90                  95      


Tyr Ala Glu Asp Gly Pro Ser Ser Thr Phe Ser Thr Leu Asn Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Lys Glu Lys Ile Glu Leu Gln Asp Leu Val Lys Val 
        115                 120                 125             


Leu Leu Gln Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Ser Glu Glu Asp Asp Gly Ser Ala Ile Asp Ser Met Gly Ile Ala 
145                 150                 155                 160 


Lys Glu Leu Tyr Glu Asn Asp Leu Thr Pro Gly Gln Trp Val Tyr Glu 
                165                 170                 175     


Ala Leu Gln Lys Gly Arg Lys Asn Val Pro Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         


Leu Gln Glu Glu Phe Lys Lys Ile Val Asn Tyr Gln Ser Glu Phe Phe 
        195                 200                 205             


Pro Asp Ile Phe Asn Ala Ser Phe Val Glu Asp Trp Met Gly Lys Ala 
    210                 215                 220                 


Ser Thr Pro Thr Lys Gln Tyr Phe Asn Lys Lys Gly Val Gln Leu Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Lys Arg Glu Glu Arg Arg Leu Gln Glu Tyr Lys Trp 
                245                 250                 255     


Arg Ala Glu Ala Val Asn Phe Lys Ile Asp Leu Ser Glu Ile Ala Leu 
            260                 265                 270         


Ile Leu Ser Gln Ile Asn Ser Gln Ile Ser Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Lys Asn Leu Thr 
    290                 295                 300                 


Val Gly Gln Tyr Leu Tyr Gln Gln Ile Lys Lys Asn Pro His Thr Arg 
305                 310                 315                 320 


Leu Lys Gly Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Arg Ile Trp Ser Val Gln Ser Ser Phe Tyr Pro Gln Leu Asn Asp Ala 
            340                 345                 350         


Leu Lys Arg Glu Val Arg Asp Ile Thr Ile Phe Phe Gln Arg Arg Leu 
        355                 360                 365             


Lys Ser Gln Lys His Leu Ile Ser Asn Cys Glu Phe Glu Asp His His 
    370                 375                 380                 


Lys Val Val Pro Lys Ser His Pro Val Phe Gln Glu Phe Arg Ile Trp 
385                 390                 395                 400 


Gln Asn Leu Asn Asn Leu Leu Leu Ile Lys Lys Asp Asn Leu Asn Glu 
                405                 410                 415     


Lys Phe Asp Leu Glu Leu Glu Ser Lys Ile Ala Leu Ala Asn Glu Leu 
            420                 425                 430         


Ala Phe Lys Arg Glu Leu Asn Val Lys Asp Ala Leu Lys Ile Leu Gly 
        435                 440                 445             


Leu Lys Pro Asn Glu Trp Glu Phe Asn Phe Thr Lys Ile Glu Gly Asn 
    450                 455                 460                 


Arg Thr Asn Gln Ala Phe Phe Asp Ala Phe Ala Lys Ile Ile Glu Leu 
465                 470                 475                 480 


Glu Asp Gly Glu Pro Ile Asp Leu Gly Asp Leu Lys Ala Asp Asp Ile 
                485                 490                 495     


Leu Asp Gln Phe Ser Glu Ala Phe Leu Arg Ile Gly Ile Asp Thr Glu 
            500                 505                 510         


Leu Leu Gln Val Asn Ser Asp Ile Glu Gly Ala Glu Tyr Glu Lys Gln 
        515                 520                 525             


Ser Tyr Ile Gln Phe Trp His Leu Leu Tyr Ser Ser Glu Asp Asp Gln 
    530                 535                 540                 


Lys Leu Lys Leu Asn Leu Ile Arg Lys Phe Gly Phe Lys Pro Glu His 
545                 550                 555                 560 


Ala Lys Ile Leu Ala Ser Ile Ser Leu Gln Asp Asp His Ala Ser Leu 
                565                 570                 575     


Ser Ser Arg Ala Ile Lys Lys Ile Leu Pro His Leu Gln Ser Gly Leu 
            580                 585                 590         


Ile Tyr Asp Lys Ala Cys Thr Tyr Ala Gly Tyr Asn His Ser Ser Ser 
        595                 600                 605             


Phe Thr Lys Asp Glu Asn Glu Lys Arg Glu Leu Arg Ala Glu Leu Glu 
    610                 615                 620                 


Leu Leu Lys Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu 
625                 630                 635                 640 


Asn Gln Met Ile Asn Val Val Asn Ala Ile Leu Lys Asp Pro Glu Leu 
                645                 650                 655     


Gly Arg Pro Asp Glu Ile Arg Val Glu Met Ala Arg Glu Leu Lys Ala 
            660                 665                 670         


Asn Ala Glu Gln Arg Lys Asn Met Thr Ser Asn Ile Ala Ser Ala Thr 
        675                 680                 685             


Arg Asp His Asp Lys Tyr Arg Glu Ile Leu Lys Ser Glu Phe Gly Leu 
    690                 695                 700                 


Lys Arg Val Thr Lys Asn Asp Leu Leu Arg Tyr Lys Leu Trp Leu Glu 
705                 710                 715                 720 


Thr Asp Gly Ile Ser Leu Tyr Thr Gly Lys Pro Ile Glu Ala Ser Lys 
                725                 730                 735     


Leu Phe Ser Lys Glu Tyr Asp Ile Glu His Ile Ile Pro Lys Ala Arg 
            740                 745                 750         


Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Ile Cys Glu Arg Gln Leu 
        755                 760                 765             


Asn Ile Asp Lys Ala Asn Val Thr Ala Phe Ser Phe Leu Gln Asn Lys 
    770                 775                 780                 


Leu Ser Ala Asp Glu Phe Glu Gln Tyr Gln Ser Arg Val Lys Ser Leu 
785                 790                 795                 800 


Tyr Gly Lys Leu Ser Lys Ala Lys Ile Gln Lys Leu Leu Met Ala Asn 
                805                 810                 815     


Asp Lys Ile Pro Glu Asp Phe Ile Ala Arg Gln Leu Gln Glu Thr Arg 
            820                 825                 830         


Tyr Ile Ser Lys Lys Ala Lys Glu Ile Leu Phe Glu Ile Ser Arg Arg 
        835                 840                 845             


Val Ser Val Thr Thr Gly Thr Ile Thr Asp Lys Leu Arg Glu Asp Trp 
    850                 855                 860                 


Gly Leu Val Glu Ile Met Lys Glu Leu Asn Trp Glu Lys Tyr Asp Lys 
865                 870                 875                 880 


Leu Gly Leu Thr Tyr Thr Ile Glu Gly Lys His Gly Glu Arg Leu Asn 
                885                 890                 895     


Lys Ile Lys Asp Trp Ser Lys Arg Asn Asp His Arg His His Ala Met 
            900                 905                 910         


Asp Ala Leu Thr Val Ala Leu Thr Lys Pro Ala Tyr Ile Gln Tyr Leu 
        915                 920                 925             


Asn Asn Leu Asn Ala Lys Gly Leu Asn Asn Lys Lys Gly Thr Glu Val 
    930                 935                 940                 


Phe Ala Ile Glu Gln Lys Tyr Leu Lys Arg Glu Asn Gly Lys Leu Cys 
945                 950                 955                 960 


Phe Ile Pro Pro Ile Glu Asn Ile Arg Ser Glu Ala Lys Lys His Leu 
                965                 970                 975     


Ser Arg Ile Leu Val Ser Tyr Lys Ala Lys Asn Lys Val Val Thr Ile 
            980                 985                 990         


Asn Lys Asn Lys Thr Lys Ser Lys  Ala Gly Leu Asn Glu  Gln Ile Ala 
        995                 1000                 1005             


Leu Thr  Pro Arg Gly Gln Leu  His Lys Glu Thr Val  Tyr Gly Lys 
    1010                 1015                 1020             


Ser Phe  His Tyr Ser Thr Lys  Phe Glu Lys Ile Gly  Ala Ser Phe 
    1025                 1030                 1035             


Asn Val  Gln Lys Ile Asn Thr  Val Ala Lys Lys Glu  Glu Arg Glu 
    1040                 1045                 1050             


Ala Leu  Leu Lys Arg Leu Ala  Glu Asn Gly Asn Asp  Pro Lys Lys 
    1055                 1060                 1065             


Ala Phe  Thr Gly Lys Asn Thr  Leu Asn Lys Met Pro  Ile Tyr Leu 
    1070                 1075                 1080             


Asp Leu  Gly Lys Asn Ile Lys  Leu Ser Glu Lys Val  Lys Thr Val 
    1085                 1090                 1095             


Val Leu  Glu Gln Asn Tyr Thr  Ile Arg Lys Asn Ile  Asp Pro Asp 
    1100                 1105                 1110             


Leu Lys  Val Asp Lys Val Ile  Asp Val Gly Ile Lys  Arg Ile Leu 
    1115                 1120                 1125             


Glu Ser  Arg Leu Glu Glu Phe  Gly Gly Asn Ala Lys  Leu Ala Phe 
    1130                 1135                 1140             


Ser Asn  Leu Glu Glu Asn Pro  Ile Trp Leu Asn Lys  Glu Lys Gly 
    1145                 1150                 1155             


Ile Ser  Ile Lys Arg Val Lys  Ile Ser Gly Val Ser  Asn Val Glu 
    1160                 1165                 1170             


Ser Leu  His Val Lys Lys Asp  His Phe Gly Glu Pro  Ile Leu Asp 
    1175                 1180                 1185             


Gln Glu  Gly Asn Glu Ile Pro  Val Asp Phe Val Ser  Thr Gly Asn 
    1190                 1195                 1200             


Asn His  His Val Ala Ile Tyr  Glu Asp Glu Asn Gly  Asn Leu Gln 
    1205                 1210                 1215             


Glu Glu  Val Val Ser Phe Phe  Glu Ala Val Val Arg  Gln Asn Gln 
    1220                 1225                 1230             


Gly Leu  Pro Ile Ile Lys Lys  Asn His Thr Leu Gly  Trp Lys Phe 
    1235                 1240                 1245             


Leu Phe  Thr Leu Lys Gln Asn  Glu Tyr Phe Val Phe  Pro Ser Asp 
    1250                 1255                 1260             


Asp Phe  Val Pro Ala Asp Val  Asp Leu Met Asp Glu  Gln Asn Tyr 
    1265                 1270                 1275             


His Leu  Ile Ser Pro Asn Leu  Phe Arg Val Gln Lys  Ile Ala Arg 
    1280                 1285                 1290             


Lys Asn  Tyr Val Phe Asn Asn  His Leu Glu Thr Lys  Ala Val Asp 
    1295                 1300                 1305             


Asn Asp  Leu Leu Lys Ser Lys  Lys Glu Leu Ser Lys  Ile Thr Tyr 
    1310                 1315                 1320             


His Phe  Tyr Gln Thr Pro Glu  His Leu Arg Gly Ile  Ile Lys Ile 
    1325                 1330                 1335             


Arg Ile  Asn His Leu Gly Lys  Ile Ile Gln Ile Gly  Glu Tyr 
    1340                 1345                 1350         


<210>  924
<211>  1352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  924

Met Asn Lys Lys Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Tyr Lys Tyr Ala Gly 
            20                  25                  30          


Lys Arg Met Trp Gly Ile Arg Leu Phe Glu Ala Ala Asp Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Met Lys Arg Ser Asn Arg Arg Arg Leu Ser Arg Lys Lys 
    50                  55                  60                  


Gln Arg Ile Asp Leu Leu Gln Glu Leu Phe Ser Glu Glu Ile Val Lys 
65                  70                  75                  80  


Lys Asp Leu Thr Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Phe 
                85                  90                  95      


Glu Asp Lys Ser Thr Gly Glu Lys Tyr Pro Leu Phe Val Asp Lys Glu 
            100                 105                 110         


Tyr Thr Asp Ile Asp Tyr His Lys Gln Tyr Pro Thr Met Tyr His Leu 
        115                 120                 125             


Arg Lys Glu Leu Ile Glu Asn Pro Glu Pro His Asp Ile Arg Leu Val 
    130                 135                 140                 


Tyr Leu Ala Cys His His Ile Leu Lys Asn Arg Gly His Phe Leu Ile 
145                 150                 155                 160 


Asn Gly Ser Ile Ser Asp Val Lys Asn Leu Ser Phe Val Cys Gly Glu 
                165                 170                 175     


Met Ile Lys Glu Phe Asn Ser Val Ser Glu Tyr Glu Ile Ser Leu Thr 
            180                 185                 190         


Asp Ile Arg Glu Leu Glu Arg Ile Leu Ser Asp Lys Lys Thr Ala Lys 
        195                 200                 205             


Ser Val Lys Ala Lys Gln Val Ala Gln Leu Phe Glu Cys Thr Pro Ala 
    210                 215                 220                 


Glu Asp Lys Glu Gln Gln Lys Cys Ala Lys Ala Ile Ile Thr Asn Leu 
225                 230                 235                 240 


Ser Lys Leu Ile Val Gly Leu Lys Gly Asn Ile Ser Lys Met Phe His 
                245                 250                 255     


Gln Pro Leu Glu Glu Ile Ser Thr Lys Ser Phe Lys Phe Thr Glu Ala 
            260                 265                 270         


Lys Tyr Glu Asp Glu Ile Met Pro Ala Ile Glu Glu Asn Tyr Pro Asp 
        275                 280                 285             


Tyr Ala Ser Val Ile Asp Arg Ile Lys Gly Val Tyr Asp Trp Ser Val 
    290                 295                 300                 


Leu Val Glu Ile Leu Asp Gly Glu Asp Tyr Leu Ser Phe Ala Lys Val 
305                 310                 315                 320 


Lys Ala Tyr Glu Lys His Gly Lys Asn Leu His Ile Leu Lys Asn Val 
                325                 330                 335     


Met Lys Lys Tyr Cys Lys Asp Glu Val Tyr Gln Ala Phe Phe Asn Gly 
            340                 345                 350         


Ile Asn Glu Lys Asn Gly Tyr Gly Asn Tyr Ile Gly Ala Ile Arg Lys 
        355                 360                 365             


Asn Gly His Thr Tyr Lys Met Asp Arg Cys Lys Ser Glu Glu Asp Phe 
    370                 375                 380                 


Tyr Lys Gly Leu Gln Lys Ile Leu Ser Thr Ile Val Pro Asp Gln Glu 
385                 390                 395                 400 


Asp Glu Glu Leu Leu Glu Lys Leu Lys Ala Glu Val Glu Asn Gln Thr 
                405                 410                 415     


Leu Leu Pro Leu Ala Arg Ser Lys Glu Asn Gly Thr Ile Pro Asn Gln 
            420                 425                 430         


Val His Leu Gln Glu Leu Met Lys Ile Leu Asp Asn Ala Lys Glu Tyr 
        435                 440                 445             


Leu Asp Phe Leu Lys Lys Glu Asp Glu Tyr Gly Thr Ile Ala Glu Lys 
    450                 455                 460                 


Ile Ile Ser Ile Ala Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu 
465                 470                 475                 480 


Ser Arg Arg His Lys Glu Glu Gly Ser Asn Ser Trp Met Ile Arg Lys 
                485                 490                 495     


Glu Glu Gly Arg Ile Tyr Pro Trp Asn Phe Gly Asp Lys Val Asp Val 
            500                 505                 510         


Glu Gln Ser Asn Lys Glu Phe Ile Glu Arg Met Thr Asn Lys Cys Thr 
        515                 520                 525             


Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Asn Ser Leu Leu Tyr Ser 
    530                 535                 540                 


Lys Tyr Met Val Leu Asn Glu Leu Asn Asn Leu Lys Ile Arg Gly Asn 
545                 550                 555                 560 


Lys Ile Ser Val Glu Leu Lys Gln Arg Ile Tyr Lys Asp Leu Phe Cys 
                565                 570                 575     


Thr Met Ala Lys Val Thr Gly Lys Arg Ile Leu Glu Tyr Leu Lys Met 
            580                 585                 590         


Glu Asp Pro Glu Ile Val Gln Glu Asp Ile Ser Gly Phe Asp Ile Asp 
        595                 600                 605             


Phe Lys Ser Ala Leu Ala Ser Tyr Leu Asp Phe Lys Lys Gln Val Val 
    610                 615                 620                 


Gly Asn Glu Ile Glu Lys Asp His Tyr Lys Glu Ile Ile Glu Glu Ile 
625                 630                 635                 640 


Ile Lys Trp Lys Thr Ile Tyr Asp Asp Asp Ser Lys Met Met Arg Arg 
                645                 650                 655     


Met Ile Glu Arg Glu Tyr Pro Glu Val Phe Asp Lys Glu Gln Ile Lys 
            660                 665                 670         


Lys Ile Cys Gln Leu Lys Tyr Ser Gly Trp Gly Asn Phe Ser Asp Arg 
        675                 680                 685             


Phe Leu Asn Gly Val Arg Gly Ala Asp Lys Glu Thr Glu Glu Ser Tyr 
    690                 695                 700                 


Thr Ile Ile Glu Ala Leu Trp Lys Thr Asn Tyr Asn Leu Met Gln Leu 
705                 710                 715                 720 


Leu Ser Lys Gln Phe Ser Phe Lys Glu Glu Ile Asp Gln Ile Asn Ala 
                725                 730                 735     


Glu Lys Ala Gly Asp Val Gly Lys Ile Asn Tyr Glu Asn Thr Val Lys 
            740                 745                 750         


Asp Leu Ile Val Ser Pro Ser Asn Lys Arg Ala Ile Trp Gln Thr Ile 
        755                 760                 765             


Gln Ile Ala Glu Glu Ile Lys Lys Val Met Lys Cys Glu Pro Glu Arg 
    770                 775                 780                 


Ile Phe Ile Glu Met Ala Arg Gly Gly Glu Lys Glu Lys Lys Arg Thr 
785                 790                 795                 800 


Val Ser Arg Lys Thr Arg Leu Gln Glu Leu Tyr Ala Gly Cys Lys Asp 
                805                 810                 815     


Asp Thr Arg Asn Trp Met Lys Glu Ile Gly Asp Arg Glu Glu Arg Glu 
            820                 825                 830         


Phe Asn Ser Arg Lys Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Lys Cys 
        835                 840                 845             


Met Tyr Thr Gly Asp Glu Ile Asp Val Asp Ala Leu Met Lys Gly Asn 
    850                 855                 860                 


Ser Asn Trp Asp Ile Asp His Ile Tyr Pro Gln Ser Arg Ile Lys Asp 
865                 870                 875                 880 


Asp Ser Leu Asp Asn Met Val Leu Val Arg Lys Asp Val Asn Asn Lys 
                885                 890                 895     


Lys Arg Asn Lys Ile Leu Ser Pro Lys Ile Arg Glu Asp Met Lys Gly 
            900                 905                 910         


Tyr Trp Thr Met Leu Cys Gln Lys Gly Leu Ile Ser Lys Arg Lys Tyr 
        915                 920                 925             


Asp Arg Leu Thr Arg Thr Thr Asp Phe Thr Asp Glu Glu Leu Ser Gly 
    930                 935                 940                 


Phe Ile Glu Arg Gln Leu Val Glu Thr Arg Gln Ser Ser Lys Ala Val 
945                 950                 955                 960 


Ala Glu Leu Leu Asn Arg Leu Tyr Lys Asp Ser Glu Val Val Tyr Val 
                965                 970                 975     


Lys Ala Gly Leu Val Ser Asp Phe Arg His Asp Asn Asp Leu Leu Lys 
            980                 985                 990         


Ser Arg Arg Val Asn Asp Tyr His  His Ala Lys Asp Ala  Phe Leu Asn 
        995                 1000                 1005             


Ile Val  Val Gly Asn Val Tyr  His Val Arg Phe Thr  Ser Asn Pro 
    1010                 1015                 1020             


Arg Lys  Trp Ile Lys Asp Asn  Arg Asp Ser Asn Tyr  Ser Ile Arg 
    1025                 1030                 1035             


Arg Val  Phe Asn Tyr Asp Val  Lys Gln Gly Asp Lys  Ile Val Trp 
    1040                 1045                 1050             


His Gly  Ser Glu Asn Gly Gly  His Ser Ile Asp Gln  Ile Lys Lys 
    1055                 1060                 1065             


Ile Met  Ser Arg Asn Asp Ile  Leu Tyr Thr Glu Tyr  Thr Tyr Cys 
    1070                 1075                 1080             


Glu Lys  Gly Gln Leu Phe Asn  Glu Thr Leu Ala Lys  Lys Gly Asn 
    1085                 1090                 1095             


Glu Lys  Ala Ile Ala Leu Lys  Lys Asn Leu Asp Pro  Val Lys Tyr 
    1100                 1105                 1110             


Gly Gly  Tyr Thr Thr Pro Lys  Thr Ser Ala Phe Ala  Phe Val Glu 
    1115                 1120                 1125             


Phe Asp  Gly Lys Lys Lys Glu  Arg Lys Asn His Ile  Val Glu Ile 
    1130                 1135                 1140             


Pro Val  Tyr Val Val Asn Ile  Ala Lys Arg Glu Pro  Asp Ala Val 
    1145                 1150                 1155             


Lys Lys  Tyr Leu Glu Glu Arg  Lys Gly Tyr Ser Asn  Val Val Ile 
    1160                 1165                 1170             


Lys Lys  Tyr Pro Ile Lys Lys  Asn Ser Leu Ile Lys  Met Asp Gly 
    1175                 1180                 1185             


Tyr Leu  Ala Arg Leu Arg Gly  Ala Asn Glu Lys Asp  Ile Ile Leu 
    1190                 1195                 1200             


Lys Asn  Ala Val Gln Leu Ile  Leu Asn Lys Asp Leu  Tyr Glu Ile 
    1205                 1210                 1215             


Val Arg  Lys Ile Glu Lys Tyr  Leu Glu Tyr Asn Leu  Gln Tyr Gln 
    1220                 1225                 1230             


Ala Asp  Ala Lys Phe Glu Gly  Phe Thr Met Lys Asp  Leu Asn Met 
    1235                 1240                 1245             


Ile Tyr  Glu Glu Leu Tyr Leu  Lys Leu Lys Glu Ser  Ile Phe Gln 
    1250                 1255                 1260             


Lys Arg  Pro Ala Gln Gln Met  Lys Thr Leu Glu Lys  Gly Lys Glu 
    1265                 1270                 1275             


Ala Phe  Ile Ser Ile Glu Asn  Leu Gly Glu Lys Ala  Lys Val Ile 
    1280                 1285                 1290             


Asn Glu  Ile Ile Ala Met Phe  Arg Cys Asp Ala Thr  Thr Val Ala 
    1295                 1300                 1305             


Asn Leu  Lys Val Ile Asn Gly  Ser Gln Asn Ala Gly  Ser Met Lys 
    1310                 1315                 1320             


Val Asn  Lys Asn Thr Leu Thr  Ser Lys Ser Leu Lys  Leu Val His 
    1325                 1330                 1335             


Gln Ser  Ile Thr Gly Leu Phe  Glu Thr Glu Glu Glu  Leu Val 
    1340                 1345                 1350         


<210>  925
<211>  1353
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  925

Met Lys Lys Lys Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Cys Lys Phe Asn Gly 
            20                  25                  30          


Lys Lys Met Trp Gly Ile Arg Leu Phe Glu Asp Ala Lys Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Thr Lys Arg Ser Asn Arg Arg Arg Ile Ala Arg Thr Lys 
    50                  55                  60                  


Gln Arg Ile Asp Leu Leu Gln Glu Leu Cys Glu Lys Ser Ile Phe Glu 
65                  70                  75                  80  


Lys Asp Pro Thr Phe Phe Ile Arg Leu Asn Glu Ser Ser Leu His Leu 
                85                  90                  95      


Glu Asp Lys Ser Thr Gly Glu Lys Tyr Pro Leu Phe Val Asp Lys Glu 
            100                 105                 110         


Tyr Thr Asp Ile Asp Tyr His Arg Glu Tyr Pro Thr Val Tyr His Leu 
        115                 120                 125             


Arg Lys Glu Leu Ile Glu Asn Pro Glu Pro His Asp Pro Arg Leu Val 
    130                 135                 140                 


Tyr Leu Ala Cys Cys His Ile Ile Lys Ser Arg Gly His Phe Leu Val 
145                 150                 155                 160 


Asn Gly Ser Ile Ser Asp Ala Lys Asp Leu Ser Phe Ile Cys Glu Asn 
                165                 170                 175     


Met Ile Asn Ser Phe Asn Ala Ala Ser Asp Tyr Lys Ile Asn Ile Gln 
            180                 185                 190         


Asp Met Thr Ala Leu Glu Thr Ile Leu Thr Asp Arg Lys Met Ala Lys 
        195                 200                 205             


Ser Val Lys Ala Lys Gln Ile Val Gln Leu Phe Lys Cys Ile Pro Cys 
    210                 215                 220                 


Glu Asp Lys Glu Gln Gln Lys Cys Cys Lys Ala Ile Ile Thr Asn Leu 
225                 230                 235                 240 


Ser Lys Leu Ile Val Gly Leu Lys Gly Asp Ile Ser Lys Ile Phe Asn 
                245                 250                 255     


Glu Ser Phe Glu Glu Ile Lys Ser Phe Lys Phe Ala Glu Thr Lys Tyr 
            260                 265                 270         


Glu Asp Glu Ile Leu Pro Thr Met Glu Glu Ile Tyr Pro Asp His Ala 
        275                 280                 285             


Glu Ile Ile Glu Lys Ile Lys Ser Ile Tyr Asp Trp Ser Ile Leu Asp 
    290                 295                 300                 


Glu Ile Leu Asp Gly Glu Glu Tyr Leu Ser Phe Ala Lys Val Lys Ala 
305                 310                 315                 320 


Tyr Glu Lys His Lys Lys Asn Leu His Asp Leu Lys Ile Leu Ile Lys 
                325                 330                 335     


Lys Tyr Cys Asp Asp Glu Thr Tyr Gln Ser Phe Phe Asn Gly Met Asn 
            340                 345                 350         


Glu Lys Asn Lys Tyr Ser Asn Tyr Ile Gly Ser Ile Arg Lys Asn Gly 
        355                 360                 365             


His Thr Tyr Lys Val Glu Lys Cys Lys Ser Ser Glu Asp Phe Tyr Lys 
    370                 375                 380                 


Glu Leu Leu Lys Leu Leu Ser Ser Val Asn Pro Gly Gln Glu Asp Glu 
385                 390                 395                 400 


Ser Leu Leu Glu Gln Ile Lys Phe Glu Thr Glu Asn Gln Thr Leu Leu 
                405                 410                 415     


Pro Leu Leu Arg Thr Lys Glu Asn Gly Thr Ile Pro Asn Gln Val His 
            420                 425                 430         


Met Gln Glu Leu Ile Lys Ile Leu Asp Asn Ala Ser Lys Tyr Leu Asp 
        435                 440                 445             


Phe Leu Asn Glu Lys Asp Glu Tyr Gly Thr Thr Ala Asp Lys Ile Ile 
    450                 455                 460                 


Ser Ile Ala Lys Phe Lys Ile Pro Tyr Tyr Val Gly Pro Leu Ser Asp 
465                 470                 475                 480 


Arg His Lys Glu Asn Gly Ser Asn Ser Trp Met Val Arg Lys Glu Asn 
                485                 490                 495     


Gly Arg Ile Tyr Pro Trp Asn Phe Asp Asp Lys Val Asp Ala Glu Lys 
            500                 505                 510         


Ser Asn Lys Glu Phe Ile Gln Arg Met Thr Asn Lys Cys Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Asn Ser Leu Leu Tyr Ser Arg Tyr 
    530                 535                 540                 


Met Val Leu Asn Glu Leu Asn Asn Leu Lys Ile Arg Gly Asn Lys Ile 
545                 550                 555                 560 


Ser Val Glu Leu Lys Gln Gln Ile Tyr Asn Asp Leu Phe Cys Thr Lys 
                565                 570                 575     


Ala Arg Val Thr Gly Lys Asn Ile Leu Asn Tyr Leu Lys Lys Glu Asp 
            580                 585                 590         


Pro Glu Leu Ala Ala Glu Asp Leu Ser Gly Phe Asp Val Asp Phe Lys 
        595                 600                 605             


Ser Ala Leu Thr Ser Tyr Leu Asp Phe Lys Lys Gln Val Val Gly Asn 
    610                 615                 620                 


Glu Ile Glu Lys Asp Tyr Tyr Lys Glu Ile Ile Glu Glu Ile Ile Lys 
625                 630                 635                 640 


Trp Lys Thr Ile Tyr Asn Glu Asp Ser Lys Met Met Lys Arg Met Ile 
                645                 650                 655     


Gly Arg Glu Tyr Pro Glu Thr Leu Asn Gln Asp Gln Ile Lys Lys Ile 
            660                 665                 670         


Cys His Leu Lys Tyr Asn Gly Trp Gly Asn Phe Ser Glu Thr Phe Leu 
        675                 680                 685             


Asn Gly Ile Lys Gly Ala Asn Lys Glu Thr Gly Glu Cys Tyr Thr Ile 
    690                 695                 700                 


Ile Glu Ala Leu Trp Lys Thr Asn Tyr Asn Leu Met Gln Leu Leu Ser 
705                 710                 715                 720 


Lys Gln Phe Ser Phe Lys Glu Glu Ile Asp Gln Ile Asn Ala Glu Lys 
                725                 730                 735     


Thr Gly Ala Ile Gly Lys Ile Thr Tyr Glu Asn Leu Val Lys Asp Leu 
            740                 745                 750         


Val Val Ser Pro Ala Asn Lys Arg Ala Ile Trp Gln Thr Ile Gln Ile 
        755                 760                 765             


Thr Glu Glu Ile Lys Lys Val Met Lys Cys Glu Pro Glu Arg Ile Phe 
    770                 775                 780                 


Ile Glu Met Ala Arg Gly Gly Glu Lys Glu Lys Lys Arg Thr Val Ser 
785                 790                 795                 800 


Arg Lys Thr Arg Leu Gln Glu Leu Tyr Thr Gly Cys Lys Asp Asp Thr 
                805                 810                 815     


Arg Asp Trp Met Lys Glu Ile Gly Asp Arg Glu Glu Arg Glu Phe Ser 
            820                 825                 830         


Ser Arg Lys Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Lys Cys Met Tyr 
        835                 840                 845             


Thr Gly Glu Glu Ile Asp Val Asp Leu Leu Met Thr Ala Asn Ser Lys 
    850                 855                 860                 


Trp Asp Ile Asp His Ile Tyr Pro Gln Ser Arg Ile Lys Asp Asp Ser 
865                 870                 875                 880 


Phe Asp Asn Met Val Leu Val Arg Lys Asp Ile Asn Asn Asn Val Lys 
                885                 890                 895     


Gly Asn Asn Ile Val Pro Ser Ser Ile Gln Lys Glu Met Thr Ser Tyr 
            900                 905                 910         


Trp Lys Met Leu Leu Gln Lys Glu Leu Ile Ser Lys Lys Lys Tyr Glu 
        915                 920                 925             


Arg Leu Thr Arg Ser Thr Asp Phe Thr Asp Glu Glu Leu Ser Gly Phe 
    930                 935                 940                 


Ile Glu Arg Gln Leu Val Glu Thr Arg Gln Ser Ser Lys Ala Val Ala 
945                 950                 955                 960 


Ala Leu Leu Asn Arg Leu Tyr Lys Asn Ser Glu Ile Val Tyr Val Lys 
                965                 970                 975     


Ala Gly Leu Val Ser Asp Phe Arg His Asp Asn Lys Leu Leu Lys Ser 
            980                 985                 990         


Arg Arg Ile Asn Asp Tyr His His  Ala Lys Asp Ala Phe  Leu Asn Ile 
        995                 1000                 1005             


Val Val  Gly Asn Cys Tyr Arg  Thr Lys Phe Thr Ala  Asn Pro Arg 
    1010                 1015                 1020             


Gln Trp  Ile Lys Lys Asn Arg  Asp Thr Asn Tyr Ser  Ile Arg Arg 
    1025                 1030                 1035             


Val Phe  Asp Phe Asp Ile Lys  Gln Gly Asp Lys Ile  Val Trp Tyr 
    1040                 1045                 1050             


Gly Asn  Lys Asn Glu Asn Gly  Lys His Thr Ile Asp  Gln Ile Lys 
    1055                 1060                 1065             


Lys Ala  Met Ser Arg Asn Asp  Ile Leu Tyr Thr Glu  Tyr Thr Tyr 
    1070                 1075                 1080             


Cys Glu  Lys Gly Lys Leu Phe  Asp Glu Thr Leu Thr  Lys Lys Gly 
    1085                 1090                 1095             


Asn Lys  Lys Ala Ile Pro Leu  Lys Lys Asn Leu Asp  Pro Leu Lys 
    1100                 1105                 1110             


Tyr Gly  Gly Tyr Thr Ser Val  Lys Thr Ser Ala Phe  Ala Phe Ile 
    1115                 1120                 1125             


Glu Phe  Asp Asp Lys Lys Lys  Glu Arg Lys Asn His  Ile Val Glu 
    1130                 1135                 1140             


Ile Pro  Ile Tyr Val Ala Asn  Ile Ala Lys Arg Glu  Pro Glu Ala 
    1145                 1150                 1155             


Val Ile  Lys Tyr Leu Glu Glu  Gln Lys Gly Tyr Val  Asn Ile Val 
    1160                 1165                 1170             


Val Lys  Lys Tyr Pro Ile Lys  Lys Asn Ser Leu Leu  Lys Ile Asp 
    1175                 1180                 1185             


Gly Tyr  Leu Ala Arg Leu Arg  Gly Gly Asp Ser Ser  Ile Ile Thr 
    1190                 1195                 1200             


Leu Lys  Asn Ala Val Gln Leu  Val Leu Asp Lys Lys  Ser Tyr Glu 
    1205                 1210                 1215             


Ile Ile  Arg Lys Val Glu Lys  Tyr Leu Asp His Asn  Ser Lys Tyr 
    1220                 1225                 1230             


Glu Ala  Asn Ser Lys Leu Glu  Lys Phe Asn Glu Asn  Asp Leu Asn 
    1235                 1240                 1245             


Ile Ile  Tyr Asn Glu Leu Cys  Leu Lys Leu Arg Asn  Ser Ile Tyr 
    1250                 1255                 1260             


Gln Lys  Arg Pro Val His Gln  Leu Glu Thr Leu Glu  Gln Gly Ile 
    1265                 1270                 1275             


Asp Asn  Phe Arg Ser Leu Glu  Ser Leu Lys Asp Lys  Ala Lys Ile 
    1280                 1285                 1290             


Ile Asn  Glu Leu Leu Ala Met  Phe Arg Cys Asp Ala  Lys Thr Gly 
    1295                 1300                 1305             


Met Asp  Leu Arg Leu Ile Gly  Gly Ala Ser Asn Ser  Gly Arg Met 
    1310                 1315                 1320             


Arg Ile  Ser Lys Ser Arg Leu  Thr Ser Lys Ser Leu  Lys Leu Ile 
    1325                 1330                 1335             


Asn Gln  Ser Val Thr Gly Leu  Phe Glu Thr Glu Glu  Glu Leu Val 
    1340                 1345                 1350             


<210>  926
<211>  1353
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  926

Met Glu Lys Asn Tyr Tyr Leu Gly Leu Asp Met Gly Thr Ser Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Met Arg Lys Lys Gly 
            20                  25                  30          


Lys Asp Leu Trp Gly Val Arg Leu Phe Lys Glu Ala Lys Thr Ser Ala 
        35                  40                  45              


Glu Arg Arg Val Lys Arg Thr Ser Arg Arg Arg Leu Gln Arg Glu Lys 
    50                  55                  60                  


Ala Arg Ile Gly Tyr Leu Lys Glu Leu Phe Ala Asp Glu Ile Asn Lys 
65                  70                  75                  80  


Val Asp Ala Gly Phe Tyr Gln Arg Met Glu Asp Ser Lys Phe Ile Ser 
                85                  90                  95      


Asp Asp Lys Thr Val His Gln Lys Phe Thr Leu Phe Ser Asp Thr Asn 
            100                 105                 110         


Tyr Thr Asp Glu Asp Tyr Tyr Asn Asp Tyr Pro Thr Ile Phe His Leu 
        115                 120                 125             


Arg Lys Lys Leu Ile Glu Ser Asp Asp Glu Tyr Asp Val Arg Met Val 
    130                 135                 140                 


Phe Leu Ala Leu Leu Asn Met Tyr Lys His Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Ala Asn Leu Ser Asp Asn Gly Met Gly Asp Ile Asn Glu Gln Tyr Asp 
                165                 170                 175     


Arg Leu Lys Glu Leu Cys Ser Glu Tyr Leu Asn Val Glu Trp Glu Asn 
            180                 185                 190         


Lys Pro Asp Lys Phe Glu Glu Ile Leu Lys Glu Cys Asn Cys Ser Asn 
        195                 200                 205             


Ser Leu Arg Asn Glu Arg Leu Leu Glu Thr Phe Asn Leu Lys Lys Thr 
    210                 215                 220                 


Asp Lys Glu Lys Cys Glu Met Leu Lys Leu Ile Cys Gly Leu Ser Gly 
225                 230                 235                 240 


Thr Leu Ser Lys Ile Phe Lys Asp Leu Asp Asp Asp Ser Glu Thr Lys 
                245                 250                 255     


Lys Met Thr Ile Ser Phe Arg Ser Ser Asn Tyr Asp Glu Glu Ala Ile 
            260                 265                 270         


Asn Val Glu Glu Ile Ile Gly Ser Glu Asn Phe Glu Ile Ile Ala Val 
        275                 280                 285             


Leu Lys Glu Ile His Asp Trp Ser Met Leu Ala Asn Ile Met Arg Gly 
    290                 295                 300                 


Lys Lys Tyr Leu Ser Val Ala Arg Val Glu Glu Tyr Glu Lys His Lys 
305                 310                 315                 320 


Asp Asp Leu Lys Ile Leu Lys Lys Val Ile Lys Glu Tyr Ser Ile Glu 
                325                 330                 335     


Glu Tyr Asn Asn Phe Phe Arg Val Met Ala Asp Asn Asn Tyr Ser Ala 
            340                 345                 350         


Tyr Val Asn Ser Val Asn Ser Asn Lys Glu Ser Thr Arg Arg Gly Ala 
        355                 360                 365             


Lys Cys Asp Asn Asp Thr Phe Phe Lys Glu Ile Lys Lys Ile Val Asn 
    370                 375                 380                 


Lys Cys Tyr Asp Ser Glu Glu Lys Glu Tyr Ile Leu Lys Glu Ile Glu 
385                 390                 395                 400 


Lys Gly Thr Phe Leu Pro Lys Gln Ile Ile Ser Ser Asn Gly Val Ile 
                405                 410                 415     


Pro Asn Gln Val His Lys Ile Glu Met Lys Lys Ile Leu Gln Asn Ala 
            420                 425                 430         


Glu Lys Tyr Leu Pro Phe Leu Thr Glu Lys Asp Asp Ser Gly Leu Ser 
        435                 440                 445             


Val Ser Glu Arg Ile Leu Gln Met Phe Ser Phe Gln Ile Pro Tyr Tyr 
    450                 455                 460                 


Ile Gly Pro Ile Ser Gly Lys Ile Gly Ser Asp Glu Lys Ser Lys Asn 
465                 470                 475                 480 


Ser Trp Ser Val Arg Lys Glu Lys Gly Arg Ile Tyr Pro Trp Asn Phe 
                485                 490                 495     


Lys Asp Lys Ile Asp Glu Lys Glu Ser Ala Glu Lys Phe Ile Glu Arg 
            500                 505                 510         


Met Val Asn His Cys Thr Tyr Leu Asn Asn Glu Met Val Leu Pro Lys 
        515                 520                 525             


Asn Ser Leu Leu Tyr Glu Lys Phe Met Val Leu Asn Glu Leu Asn Asn 
    530                 535                 540                 


Leu Lys Ile Asn Asn Glu Asn Ile Ser Val Glu Leu Lys Gln Ser Leu 
545                 550                 555                 560 


Tyr Asn Asn Leu Phe Lys Thr Gly Lys Lys Val Thr Ala Lys Gly Ile 
                565                 570                 575     


Lys Ser Tyr Leu Glu Arg Asn Gly Leu Cys Glu Pro Asn Glu Asp Ile 
            580                 585                 590         


Asn Leu Thr Gly Ile Asp Gly Asp Phe Thr Asn Arg Leu Ser Asn Tyr 
        595                 600                 605             


Ala Lys Phe Lys Gly Ile Phe Asn Thr Asp Glu Leu Thr Tyr Glu Gln 
    610                 615                 620                 


Trp Asn Met Ala Glu Lys Ile Ile Lys Trp Ser Thr Ile Tyr Gly Asp 
625                 630                 635                 640 


Ser Lys Lys Phe Leu Glu Glu Lys Ile Arg Asn Glu Tyr Ser Glu Lys 
                645                 650                 655     


Leu Asp Ser Gln Gln Ile Lys Arg Ile Leu Gly Ile Lys Phe Arg Asp 
            660                 665                 670         


Trp Gly Arg Leu Ser Glu Glu Leu Leu Asn Met Glu Gly Ala Asp Val 
        675                 680                 685             


Asn Thr Gly Glu Ile Lys Ser Val Ile Gly Arg Met Trp Asp Glu Asn 
    690                 695                 700                 


Tyr Asn Leu Met Glu Leu Ile Ala Thr Asp Asn Phe Thr Tyr Lys Gln 
705                 710                 715                 720 


Val Ile Lys Glu Lys Gln Asn Gly Leu Asp Lys Ser Leu Ser Glu Ile 
                725                 730                 735     


Asp Tyr Asp Asp Leu Asp Glu Leu Tyr Val Ser Ala Pro Val Lys Arg 
            740                 745                 750         


Met Ile Trp Gln Thr Leu Leu Ile Ile Lys Glu Leu Val Ser Val Ile 
        755                 760                 765             


Gly Tyr Glu Pro Thr Arg Val Phe Val Glu Met Ala Lys Asp Val Asn 
    770                 775                 780                 


Thr Glu Lys Lys Arg Thr Asp Ser Arg Lys Lys Lys Phe Leu Asp Leu 
785                 790                 795                 800 


Tyr Lys Gln Cys Glu Lys Asp Glu Arg Asp Phe Trp Ile Lys Glu Ile 
                805                 810                 815     


Asp Asp Lys Ser Glu Ala Glu Phe Arg Lys Lys Lys Leu Tyr Leu Tyr 
            820                 825                 830         


Tyr Thr Gln Lys Gly Arg Cys Met Tyr Thr Gly Glu Gln Ile Asn Pro 
        835                 840                 845             


Leu Asp Leu Thr Asn Asp Asn Leu Tyr Asp Ile Asp His Ile Tyr Pro 
    850                 855                 860                 


Arg His Phe Val Lys Asp Asp Ser Ile Glu Asn Asn Leu Val Leu Val 
865                 870                 875                 880 


Lys Lys Glu Lys Asn Ala His Lys Ser Asp Thr Phe Pro Ile Glu Asp 
                885                 890                 895     


Asp Ile Arg Lys Asn Gln Tyr Ala Phe Trp Lys Met Leu Tyr Glu Lys 
            900                 905                 910         


Asn Phe Ile Thr Lys Val Lys Tyr Gln Arg Leu Thr Arg Lys Glu Pro 
        915                 920                 925             


Phe Ser Asp Glu Glu Lys Ala Asn Phe Ile Met Arg Gln Ile Val Glu 
    930                 935                 940                 


Thr Arg Gln Gly Thr Lys Val Ile Thr Asp Ile Leu Glu Lys Ser Phe 
945                 950                 955                 960 


Asp Lys Thr Gln Ile Val Tyr Val Lys Ala Gly Asn Val Ser Leu Phe 
                965                 970                 975     


Arg Gln Lys Tyr Glu Cys Ile Lys Cys Arg Glu Ile Asn Asp Phe His 
            980                 985                 990         


His Ala Asn Asp Ala Tyr Leu Asn  Ile Val Val Gly Asn  Val Tyr Asp 
        995                 1000                 1005             


Thr Lys  Phe Thr Ser Asp Pro  Arg Asn Phe Ile Lys  Glu Tyr Arg 
    1010                 1015                 1020             


Lys Asn  Pro Gln Lys Asn Glu  Tyr His Met Tyr Lys  Leu Phe Asp 
    1025                 1030                 1035             


Tyr Asn  Val Gln Arg Gly Asp  Arg Val Ala Trp Thr  Ala Ser Asn 
    1040                 1045                 1050             


Gly Glu  Ser Met Lys Val Val  Lys Gly Thr Leu Lys  Lys Asn Thr 
    1055                 1060                 1065             


Pro Leu  Val Thr Phe Met Ser  Tyr Glu Glu His Gly  Gly Leu Tyr 
    1070                 1075                 1080             


Asp Gln  Asn Ile Ile Ser Ala  Lys Asp Ile Glu Lys  Val Lys Gly 
    1085                 1090                 1095             


Lys Gly  Tyr Leu Pro Ile Lys  Ser Ser Asp Ser Arg  Leu Asn Asn 
    1100                 1105                 1110             


Thr Glu  Lys Tyr Gly Gly Tyr  Ala Lys Val Ser Gly  Ala Tyr Phe 
    1115                 1120                 1125             


Phe Val  Val Glu His Thr Val  Lys Asn Val Arg Ile  Arg Thr Ile 
    1130                 1135                 1140             


Glu Pro  Met Pro Cys Tyr Leu  Thr Lys Val Leu Thr  Thr Lys Glu 
    1145                 1150                 1155             


Lys Ile  Glu Asp Tyr Cys Cys  Asn Ile Leu Lys Tyr  Asp Ser Pro 
    1160                 1165                 1170             


Val Val  Lys Tyr Glu Lys Ile  Lys Met Tyr Ser Leu  Ile Lys Val 
    1175                 1180                 1185             


Asn Gly  Tyr Pro Met Tyr Leu  Thr Gly Arg Thr Gly  Asn Gln Leu 
    1190                 1195                 1200             


Ile Val  Ser Asn Gly Ile Gln  Leu Val Leu Ser Tyr  Asp Glu Ala 
    1205                 1210                 1215             


Lys Tyr  Ile Lys Asp Leu Leu  Lys Ile Asn Gln Glu  Glu Phe Asp 
    1220                 1225                 1230             


Gln Asn  Arg Leu Glu Asn Leu  Gly Ile Asn Lys Phe  Arg Asn Glu 
    1235                 1240                 1245             


Lys Leu  Tyr Asp Thr Leu Asn  Lys Lys Tyr Thr Asn  Glu Ile Tyr 
    1250                 1255                 1260             


Lys Lys  Arg Pro Asn Ser Met  Gly Asn Lys Met Ile  Glu Trp Lys 
    1265                 1270                 1275             


Asn Thr  Phe Gln Asn Leu Ser  Ile Lys Asp Gln Thr  Glu Val Leu 
    1280                 1285                 1290             


Leu Gln  Leu Ile Gln Leu Gly  Gln Lys Gln Asn Ser  Gly Val Asn 
    1295                 1300                 1305             


Leu Glu  Lys Leu Gly Ala Ser  Lys Lys Thr Gly Val  Ser Leu Val 
    1310                 1315                 1320             


Ser Lys  Lys Ile Thr Asp Lys  Glu Ser Phe Glu Leu  Ile Asn Lys 
    1325                 1330                 1335             


Ser Ile  Thr Gly Leu Tyr Glu  Asn Lys Val Asp Leu  Leu Arg Val 
    1340                 1345                 1350             


<210>  927
<211>  1353
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  927

Met Ala Ala Phe Lys Tyr Tyr Leu Gly Leu Asp Met Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Thr Asp Pro Asn Tyr Asn Leu Leu Lys Ala Lys 
            20                  25                  30          


Gly Lys Asp Leu Trp Gly Ile Arg Glu Phe Asn Glu Ala Ser Thr Ala 
        35                  40                  45              


Val Glu Arg Arg Thr His Arg Ile Ser Arg Arg Arg Arg Gln Arg Glu 
    50                  55                  60                  


Gln Val Arg Ile Gly Leu Leu Lys Asp Tyr Phe His Asp Ala Ile Gly 
65                  70                  75                  80  


Glu Val Asp Pro Asp Phe Phe Gln Arg Leu Ala Asn Ser Lys Tyr His 
                85                  90                  95      


Leu Glu Asp Lys Asp Thr Glu Val Arg Tyr Lys Asn Asn Val Phe Asn 
            100                 105                 110         


Asp Asp Asp Tyr Thr Asp Lys Asp Tyr Phe Asp Gln Tyr Pro Thr Ile 
        115                 120                 125             


Tyr His Leu Arg Lys Glu Leu Ile Glu Ser Thr Asp Lys His Asp Val 
    130                 135                 140                 


Arg Leu Val Phe Leu Ala Leu Leu Asn Met Phe Lys His Arg Gly His 
145                 150                 155                 160 


Phe Leu Asn Ser Gly Leu Gly Glu Asn Ser Gly Glu Asn Asn Ile Asn 
                165                 170                 175     


Asn Ala Tyr Leu Glu Leu Thr Asn Leu Leu Ser Glu Phe Thr Gln Tyr 
            180                 185                 190         


Asn Leu Asn Glu Thr Ile Asp Gly Lys Lys Ile Glu Asp Ile Leu Ser 
        195                 200                 205             


Arg Arg Asp Met Ser Arg Thr Arg Lys Ala Glu Gly Leu Ala Glu Ile 
    210                 215                 220                 


Leu Gly Val Asp Tyr Lys Asn Lys Pro Tyr Lys Glu Leu Ile Arg Gly 
225                 230                 235                 240 


Leu Cys Gly Leu Lys Phe Asn Ala Cys Ala Ile Phe Pro Glu Ile Gln 
                245                 250                 255     


Ser Asp Glu Val Pro Lys Leu Asp Ile Cys Leu Ser Glu Ala Ser Phe 
            260                 265                 270         


Asp Glu Lys Ser Asp Glu Ile Ala Asn Ile Leu Gly Glu Asp Tyr Phe 
        275                 280                 285             


Glu Ile Ile Met Ala Met Lys Asp Ile Tyr Asp Ile Gly Ser Leu Ala 
    290                 295                 300                 


Gly Ile Met Lys Gly His Asn Tyr Leu Ser Gln Ala Arg Val Thr Ser 
305                 310                 315                 320 


Tyr Glu Lys His Lys Glu Asp Leu Arg Leu Leu Lys Asn Val Ile Arg 
                325                 330                 335     


Lys Tyr Cys Ser Lys Glu Asp Tyr Asp Asn Phe Phe Asn Ser Glu Ala 
            340                 345                 350         


Asp Gly Ser Tyr Ala Ser Tyr Val Gly Ser Tyr Asn Ser Lys Thr Lys 
        355                 360                 365             


Gln Arg Arg Val Gly Asn Lys Arg Ser Ser Glu Glu Leu Tyr Lys Ala 
    370                 375                 380                 


Ile Lys Lys Ile Leu Lys Asn Val Pro Ala Glu Asp Ala Asp Val Gln 
385                 390                 395                 400 


Arg Ile Leu Met Ser Ile Glu Asn Glu Thr Phe Leu Pro Lys Gln Leu 
                405                 410                 415     


Thr Ala Ser Asn Gly Val Ile Pro Asn Gln Val His Ala Lys Glu Ile 
            420                 425                 430         


Lys Lys Ile Leu Ser Asn Ala Glu Arg Tyr Leu Pro Phe Leu Arg Glu 
        435                 440                 445             


Lys Asp Glu Thr Gly Leu Ser Val Ser Glu Lys Ile Met Lys Leu Phe 
    450                 455                 460                 


Ser Phe Gln Ile Pro Tyr Tyr Val Gly Pro Thr Thr Glu Lys Ser Ser 
465                 470                 475                 480 


Lys Asp Gly Gly Asn Gly Trp Val Val Arg Lys Glu Glu Gly Gln Val 
                485                 490                 495     


Leu Pro Trp Asn Ile Asp Glu Lys Ile Asp Met Lys Ala Thr Ser Glu 
            500                 505                 510         


Ala Phe Ile Ser Arg Met Val Arg Arg Cys Thr Tyr Ile Ser Gly Glu 
        515                 520                 525             


Pro Val Leu Pro Lys Ser Ser Leu Glu Tyr Gln Ser Phe Cys Val Leu 
    530                 535                 540                 


Asn Glu Ile Asn Asn Ile Lys Ile Asp Gly Glu Arg Ile Ser Val Glu 
545                 550                 555                 560 


Leu Lys Gln Asp Ile Tyr Lys Glu Val Phe Gln Lys Gly Lys Arg Val 
                565                 570                 575     


Thr Lys Lys Gln Leu Cys Lys Tyr Leu His Thr Arg Gly Ile Ile Glu 
            580                 585                 590         


Ala Glu Glu Gln Val Thr Gly Ile Asp Val Thr Ile Asn Asn Ala Leu 
        595                 600                 605             


Thr Thr Tyr Gly Lys Phe Lys Ala Ile Leu Gly Asp Asp Ile Ser Lys 
    610                 615                 620                 


Asp Ser Val Gln Lys Met Val Glu Asn Ile Val Phe Trp Cys Thr Val 
625                 630                 635                 640 


Tyr Gly Asp Ser Lys Lys Phe Leu Lys Asp Arg Ile Glu Glu Lys Tyr 
                645                 650                 655     


Gly Glu Lys Leu Thr Lys Glu Gln Ile Lys Arg Ile Val Gly Phe Lys 
            660                 665                 670         


Phe Lys Asp Trp Gly Asn Leu Ser Lys Asn Phe Leu Glu Leu Ser Gly 
        675                 680                 685             


Ala Asp Val Ser Thr Gly Glu Ser Val Ser Ile Ile Arg Ala Leu Trp 
    690                 695                 700                 


Asn Asn Asn Leu Asn Leu Met Glu Leu Ile Asn Ser Arg Leu Tyr Asn 
705                 710                 715                 720 


Tyr Lys Glu Arg Leu Ala Glu Tyr Gln Asn Thr Met Met Lys Thr Leu 
                725                 730                 735     


Ser Asp Ile Glu Ala Glu Asp Leu Asp Glu Tyr Tyr Phe Ser Ala Pro 
            740                 745                 750         


Val Lys Arg Met Ile Trp Gln Thr Ile Leu Ile Ile Lys Glu Leu Val 
        755                 760                 765             


Lys Val Leu Gly Cys Glu Pro Asp Arg Ile Phe Val Glu Met Thr Arg 
    770                 775                 780                 


Arg Pro Asp Glu Arg Lys Met Arg Thr Glu Ser Arg Arg Lys Lys Phe 
785                 790                 795                 800 


Glu Glu Leu Tyr Lys Lys Val Lys Asp Glu Asp Val Asp Trp Met Lys 
                805                 810                 815     


Val Ile Ala His Ala Asp Glu Thr Gly Ser Ile Arg Ser Lys Lys Met 
            820                 825                 830         


Tyr Leu Tyr Leu Thr Gln Lys Gly Arg Cys Met Tyr Thr Gly Lys His 
        835                 840                 845             


Ile Glu Leu Ser Asp Leu Phe Asn Asn Asn Leu Tyr Asp Ile Asp His 
    850                 855                 860                 


Val Tyr Pro Arg His Phe Val Lys Asp Asp Asn Ile Asp Asn Asn Leu 
865                 870                 875                 880 


Val Leu Val Cys Lys Glu Lys Asn Ala His Lys Ser Asp Ile Tyr Pro 
                885                 890                 895     


Leu Glu Asp Glu Ile Leu Ala Ser Gln Lys Asn Met Trp Ser Glu Leu 
            900                 905                 910         


Arg Arg Ser Gly Phe Ile Thr Glu Glu Lys Tyr Asn Arg Leu Ile Gly 
        915                 920                 925             


Arg Asn Ser Phe Thr Asp Glu Gln Lys Ala Gly Phe Ile Ala Arg Gln 
    930                 935                 940                 


Leu Val Glu Thr Ser Gln Gly Thr Lys Gly Val Ala Asn Ile Leu Gln 
945                 950                 955                 960 


Gln Leu Leu Pro Glu Ser Lys Ile Val Tyr Ala Lys Ala Ser Asn Val 
                965                 970                 975     


Ser Glu Phe Arg Asn Thr Arg Asp Ile Pro Lys Ser Arg Leu Ile Asn 
            980                 985                 990         


Glu Phe His His Ala His Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn 
        995                 1000                 1005             


Val Tyr  Tyr Val Lys Phe Thr  Gln Asn Pro Leu Asn  Phe Ile Lys 
    1010                 1015                 1020             


Asn Asp  Tyr Asp Arg Asp Lys  Thr Lys Asn Asn Tyr  Asn Leu Ser 
    1025                 1030                 1035             


Lys Met  Phe Asp Trp Asp Val  Glu Arg Asn Gly Glu  Val Ala Trp 
    1040                 1045                 1050             


Ile Ala  Gln Lys Lys Asp Gly  Glu Ala Gly Thr Ile  Ala Thr Val 
    1055                 1060                 1065             


Lys Lys  Val Leu Gly Arg Asn  Thr Pro Leu Met Thr  Arg Tyr Ser 
    1070                 1075                 1080             


Phe Glu  Gly Lys Gly Gly Leu  Thr Asn Glu Thr Leu  Tyr Ser Ala 
    1085                 1090                 1095             


Lys Lys  Ala Lys Glu Glu Gly  Tyr Ile Pro Phe Lys  Ser Ser Asp 
    1100                 1105                 1110             


Glu Lys  Met Lys Asp Val Thr  Lys Tyr Gly Gly Phe  Thr Ser Val 
    1115                 1120                 1125             


Thr Gly  Ala Tyr Phe Phe Leu  Val Glu His Thr Glu  Lys Lys Lys 
    1130                 1135                 1140             


Arg Ser  Arg Met Ile Glu Ser  Val Pro Leu Tyr Leu  Ala Gln Lys 
    1145                 1150                 1155             


Ile Glu  Gln Asn Pro His Glu  Leu Glu Lys Tyr Cys  Glu Gln Val 
    1160                 1165                 1170             


Leu Gly  Leu Ile Asp Phe Asp  Ile Arg Val Arg Lys  Ile Lys Ile 
    1175                 1180                 1185             


Gly Ser  Leu Ile Lys Arg Lys  Gly Tyr Phe Phe Tyr  Ile Thr Gly 
    1190                 1195                 1200             


Lys Thr  Gly His Arg Leu Ile  Leu Arg Asn Ala Val  Asn Met Cys 
    1205                 1210                 1215             


Leu Lys  Lys Glu Trp Val Lys  Tyr Ile Ser Lys Leu  Glu Lys Ala 
    1220                 1225                 1230             


Val Asp  Lys Asn Asn Val Glu  Gly Ile Ile Thr Lys  Glu Lys Asn 
    1235                 1240                 1245             


Glu Leu  Leu Tyr Ser Glu Leu  Ile Glu Lys Phe Lys  Asn Ser Ile 
    1250                 1255                 1260             


Phe Ser  Asn Arg Pro Asn Pro  Val Gly Asp Lys Leu  Asp Glu Ala 
    1265                 1270                 1275             


Lys Glu  Lys Phe Ile Asn Leu  Asp Ile Glu Lys Gln  Cys Ile Val 
    1280                 1285                 1290             


Leu Cys  Gln Ile Leu Gln Leu  Ser Ser Val Leu Gly  Ser Glu Cys 
    1295                 1300                 1305             


Asp Met  Thr Leu Leu Gly Glu  Ser Ala His Cys Gly  Lys Ile Leu 
    1310                 1315                 1320             


Ile Ser  Lys Lys Ile Gly Asp  Glu Asp Ile Tyr Leu  Ile Asn Gln 
    1325                 1330                 1335             


Ser Val  Thr Gly Leu Tyr Glu  Lys Lys Ile Asn Leu  Arg Thr Val 
    1340                 1345                 1350             


<210>  928
<211>  1354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  928

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ile Asn Glu Ala Glu Asn Glu Ile Glu Val Ser Ser Ile Val 
            20                  25                  30          


Asn Thr Gly Val Arg Ile Val Pro Leu Thr Thr Asp Glu Glu Ser Asp 
        35                  40                  45              


Phe Lys Lys Gly Asn Thr Ile Ser Ile Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Gly Leu Gln Arg Phe Lys Gln Arg Arg Glu Ala 
65                  70                  75                  80  


Leu Leu Glu Thr Phe Asn Arg Ile Lys Phe Ile Ala Thr Asp Phe Val 
                85                  90                  95      


Tyr Ala Glu Thr Gly Ala Ala Thr Thr Phe Ser Ser Tyr Gln Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Val Glu Ala Val Thr Lys Glu Glu Leu Val Lys Val 
        115                 120                 125             


Leu Leu Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Thr Ala Glu Glu Gly Asp Ala Ile Asp Gly Met Lys Ile Ala Lys 
145                 150                 155                 160 


Glu Ile Phe Glu Asn Asn Leu Thr Pro Gly Gln Trp Val Tyr Asn Ser 
                165                 170                 175     


Leu Ser Lys Gly Gly Lys Phe Ile Pro Asp Phe Tyr His Ser Asp Leu 
            180                 185                 190         


Gln Lys Glu Leu Glu Lys Ile Val Arg Phe Gln Asn Gln Phe His Ser 
        195                 200                 205             


Glu Gln Ile Asn Asp Lys Leu Leu Ala Asp Ile Gln Gly Lys Ser Arg 
    210                 215                 220                 


Thr Gln Thr Ser Gln His Phe Ser Lys Thr Leu Asn Ile Pro Leu Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Thr Arg Glu Glu Thr Lys Leu Gln His Tyr Gln Trp 
                245                 250                 255     


Arg Asn Glu Ala Leu Ala Asn Glu Leu Asp Met Lys Ile Leu Ala Phe 
            260                 265                 270         


Ile Ile Thr Glu Ile Asn Asn Gln Ile Asn Gln Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Gln Glu Thr 
    290                 295                 300                 


Val Gly Gln Tyr Gln Tyr Lys Gln Leu Gln Glu Asn Val His Ala Lys 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Lys Ile Trp Glu Thr Gln Ala Lys Phe His Ser Glu Leu Asn Glu Lys 
            340                 345                 350         


Leu Lys Ala Glu Ile Arg Asp Ile Thr Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys His Leu Val Ser Phe Cys Glu Phe Glu Lys Gly His 
    370                 375                 380                 


Lys Ala Ile Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Arg Ile Trp 
385                 390                 395                 400 


Gln Asn Leu Asn Asn Val Val Val Lys Asn Glu Thr Thr Lys Glu Val 
                405                 410                 415     


Phe Glu Leu Ser Glu Glu Leu Lys Gln Leu Ile Ala Glu Glu Leu Gln 
            420                 425                 430         


Phe Lys Glu Ser Met Thr Asp Val Gln Met Leu Thr Phe Cys Glu Leu 
        435                 440                 445             


Lys Lys Gly Thr His Thr Val Asn Phe Lys Lys Ile Glu Gly Asn Arg 
    450                 455                 460                 


Thr Asn Thr Ser Ile Tyr Lys Ala Phe Glu Lys Ile Leu Glu Val Glu 
465                 470                 475                 480 


Gly Tyr Glu Ile Asp Phe Ser Lys Met Lys Pro Leu Glu Ile Lys Glu 
                485                 490                 495     


Thr Val Ala Pro Ile Phe Glu Thr Leu Asn Ile Asn Thr Val Ile Leu 
            500                 505                 510         


Asp Phe Asp Pro Thr Ile Gln Gly Asn Asp Phe Asp Lys Gln Pro Tyr 
        515                 520                 525             


Tyr Gln Leu Trp His Leu Leu Tyr Ser Ala Glu Asp Asp Asp Lys Leu 
    530                 535                 540                 


Lys Glu Thr Leu Lys Thr Lys Phe Gly Phe Lys Glu Asn His Leu Pro 
545                 550                 555                 560 


Phe Ile Leu Asn Ile Asn Leu Gln Ala Asp Tyr Gly Ser Leu Ser Ala 
                565                 570                 575     


Lys Ala Ile Lys Lys Ile Leu Pro His Leu Met Asp Gly His Ile Tyr 
            580                 585                 590         


Asp Lys Ala Cys Leu Met Val Gly Tyr Asn His Ser Ser Ser Leu Thr 
        595                 600                 605             


Thr Ala Gln Asn Asn Asp Arg Val Leu Lys Asp Thr Leu Glu Leu Leu 
    610                 615                 620                 


Lys Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln 
625                 630                 635                 640 


Met Ile Asn Val Ile Asn Ala Ile Met Lys Asp Pro Ala Met Gly Lys 
                645                 650                 655     


Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Ala Asn Asn 
            660                 665                 670         


Glu Gln Arg Lys Lys Ala Thr Ser Asp Ile Asn Thr Ala Thr Thr Glu 
        675                 680                 685             


His Glu Arg Ile Arg Lys Leu Leu His Ser Glu Phe Asn Ile Pro Arg 
    690                 695                 700                 


Val Thr Arg Asn Asp Ile Ile Arg Tyr Lys Leu Trp Leu Glu Thr Gly 
705                 710                 715                 720 


Gly Ile Ser Leu Tyr Thr Gly Asn Pro Ile Lys Ala Ser Asp Leu Phe 
                725                 730                 735     


Ser Lys Asn Tyr Asp Ile Glu His Ile Ile Pro Lys Ser Arg Leu Phe 
            740                 745                 750         


Asp Asp Ser Phe Ser Asn Lys Thr Leu Cys Glu Arg Gln Leu Asn Ile 
        755                 760                 765             


Asp Lys Ser Asn Lys Thr Ala Tyr Ser Phe Leu Gln Glu Lys Leu Ser 
    770                 775                 780                 


Glu Asn Asp Phe Ala Ala Tyr Glu Asn Arg Val Lys Asp Leu Phe Lys 
785                 790                 795                 800 


Thr Gly Lys Ile Lys Ser Tyr Thr Lys Tyr Lys Lys Leu Leu Met Ala 
                805                 810                 815     


Asp Asn Glu Ile Pro Glu Gly Phe Ile Asp Arg Gln Leu Arg Glu Thr 
            820                 825                 830         


Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Leu Glu Val Ser Arg 
        835                 840                 845             


Asn Val Thr Ala Thr Ile Gly Ser Val Thr Asp Lys Leu Arg Gln Asp 
    850                 855                 860                 


Trp Glu Leu Val Asp Val Met Lys Glu Leu Asn Trp Glu Lys Tyr Asp 
865                 870                 875                 880 


Lys Leu Gly Leu Thr His Ile Glu Glu Gly Lys Asn Gly Glu Arg Leu 
                885                 890                 895     


Ser Lys Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala 
            900                 905                 910         


Met Asp Ala Ile Thr Val Ala Phe Thr Lys Pro Ala Tyr Ile Gln Tyr 
        915                 920                 925             


Leu Asn Asn Leu Asn Ala Lys Thr Gln Gly Glu Asn Lys Ala Asn Ser 
    930                 935                 940                 


Ile Ile Gly Ile Glu Asn Lys His Leu Tyr Arg Asp Lys Asn Asn Lys 
945                 950                 955                 960 


Leu Arg Phe Ile Ser Pro Met Lys Asn Phe Arg Glu Glu Ala Lys Lys 
                965                 970                 975     


Gln Leu Glu Ser Ile Leu Ile Ser Tyr Lys Ala Lys Asn Lys Val Val 
            980                 985                 990         


Thr Lys Asn Lys Asn Thr Thr Lys  Lys Ser Gly Gly Thr  Asn Gln Lys 
        995                 1000                 1005             


Ile Gln  Leu Thr Pro Arg Gly  Arg Leu His Lys Glu  Thr Val Tyr 
    1010                 1015                 1020             


Gly Lys  Leu Gln Gln Tyr Ala  Thr Lys Glu Glu Lys  Val Asn Ala 
    1025                 1030                 1035             


Ser Phe  Thr Glu Asp Tyr Ile  Gln Lys Val Ala Lys  Lys Glu Tyr 
    1040                 1045                 1050             


Arg Glu  Ala Leu Leu Lys Arg  Leu Glu Glu Asn Asp  Asn Asp Pro 
    1055                 1060                 1065             


Lys Lys  Ala Phe Thr Gly Lys  Asn Ala Leu Asn Lys  Thr Pro Ile 
    1070                 1075                 1080             


Tyr Ile  Ser Leu Lys Asp Asn  Ile Ile Val Pro Glu  Lys Val Lys 
    1085                 1090                 1095             


Thr Val  Trp Leu Glu Thr Asp  Tyr Thr Ile Arg Lys  Asp Ile Thr 
    1100                 1105                 1110             


Pro Asp  Leu Lys Ile Asp Lys  Val Ile Asp Val Gly  Ile Lys Arg 
    1115                 1120                 1125             


Ile Leu  Gln Asn Arg Leu Asn  Glu Phe Asn Gly Asp  Pro Lys Lys 
    1130                 1135                 1140             


Ala Phe  Val Asn Leu Gly Glu  Ser Pro Ile Trp Leu  Asn Lys Glu 
    1145                 1150                 1155             


Lys Gly  Ile Ala Ile Lys Arg  Val Thr Ile Ser Gly  Val Ser Asn 
    1160                 1165                 1170             


Ala Gln  Ala Leu His Thr Lys  Lys Asp His Leu Gly  Asn Glu Ile 
    1175                 1180                 1185             


Leu Asp  Lys Asn Gly Asn Pro  Ile Pro Val Asp Phe  Ile Ser Thr 
    1190                 1195                 1200             


Gly Asn  Asn His His Val Ala  Ile Tyr Arg Asp Glu  Lys Gly Asn 
    1205                 1210                 1215             


Leu Gln  Glu Glu Val Val Ser  Phe Tyr Asp Ala Val  Ile Arg Arg 
    1220                 1225                 1230             


Asn Leu  Gly Leu Ser Val Ile  Asn Lys Asn His Glu  Lys Gly Trp 
    1235                 1240                 1245             


Glu Phe  Leu Phe Ser Met Lys  Gln Asn Glu Phe Phe  Ile Phe Pro 
    1250                 1255                 1260             


Ser Asp  Gly Phe Asn Pro His  Glu Ile Asp Leu Leu  Asn Pro Asn 
    1265                 1270                 1275             


Asn Tyr  His Leu Ile Ser Pro  Asn Ile Phe Arg Val  Gln Lys Ile 
    1280                 1285                 1290             


Ser Thr  Lys Asn Tyr Met Phe  Asn His His Leu Glu  Thr Lys Ala 
    1295                 1300                 1305             


Val Asp  Gly Glu Met Leu Lys  Ser Lys Lys Glu Leu  Ser Lys Thr 
    1310                 1315                 1320             


Ser Tyr  His Phe Ile Gln Thr  Pro Thr Asn Leu Asn  Gly Ile Ile 
    1325                 1330                 1335             


Lys Val  Arg Ile Asn His Leu  Gly Lys Ile Val His  Leu Gly Glu 
    1340                 1345                 1350             


Tyr 
    


<210>  929
<211>  1354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  929

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ile Asn Glu Ala Glu Asn Glu Ile Glu Val Ser Ser Ile Val 
            20                  25                  30          


Asn Thr Gly Val Arg Ile Val Pro Leu Thr Thr Asp Glu Glu Ser Asp 
        35                  40                  45              


Phe Lys Lys Gly Asn Thr Ile Ser Ile Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Gly Leu Gln Arg Phe Lys Gln Arg Arg Glu Ala 
65                  70                  75                  80  


Leu Leu Glu Thr Phe Asn Arg Ile Lys Phe Ile Ala Thr Asp Phe Val 
                85                  90                  95      


Tyr Ala Glu Thr Gly Ala Ala Thr Thr Phe Ser Ser Tyr Gln Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Val Glu Ala Val Thr Lys Glu Glu Leu Val Lys Val 
        115                 120                 125             


Leu Leu Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Thr Ala Glu Glu Gly Asp Ala Ile Asp Gly Met Lys Ile Ala Lys 
145                 150                 155                 160 


Glu Ile Phe Glu Asn Asn Leu Thr Pro Gly Gln Trp Val Tyr Asn Ser 
                165                 170                 175     


Leu Ser Lys Gly Gly Lys Phe Ile Pro Asp Phe Tyr His Ser Asp Leu 
            180                 185                 190         


Gln Lys Glu Leu Glu Lys Ile Val Arg Phe Gln Asn Gln Phe His Ser 
        195                 200                 205             


Glu Gln Ile Asn Asp Lys Leu Leu Ala Asp Ile Gln Gly Lys Ser Arg 
    210                 215                 220                 


Thr Gln Thr Ser Gln His Phe Ser Lys Thr Leu Asn Ile Pro Leu Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Thr Arg Glu Glu Thr Lys Leu Gln His Tyr Gln Trp 
                245                 250                 255     


Arg Asn Glu Ala Leu Ala Asn Glu Leu Asp Met Lys Ile Leu Ala Phe 
            260                 265                 270         


Ile Ile Thr Glu Ile Asn Asn Gln Ile Asn Gln Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Gln Glu Thr 
    290                 295                 300                 


Val Gly Gln Tyr Gln Tyr Lys Gln Leu Gln Glu Asn Val His Ala Lys 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Lys Ile Trp Glu Thr Gln Ala Lys Phe His Ser Glu Leu Asn Glu Lys 
            340                 345                 350         


Leu Lys Ala Glu Ile Arg Asp Ile Thr Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys His Leu Val Ser Phe Cys Glu Phe Glu Lys Gly His 
    370                 375                 380                 


Lys Ala Ile Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Arg Ile Trp 
385                 390                 395                 400 


Gln Asn Leu Asn Asn Val Val Val Lys Asn Glu Thr Thr Lys Glu Val 
                405                 410                 415     


Phe Glu Leu Ser Glu Glu Leu Lys Gln Leu Ile Ala Glu Glu Leu Gln 
            420                 425                 430         


Phe Lys Glu Ser Met Thr Asp Val Gln Met Leu Thr Phe Cys Glu Leu 
        435                 440                 445             


Lys Lys Gly Thr His Thr Val Asn Phe Lys Lys Ile Glu Gly Asn Arg 
    450                 455                 460                 


Thr Asn Thr Cys Ile Tyr Lys Ala Phe Glu Lys Ile Leu Glu Val Glu 
465                 470                 475                 480 


Gly Tyr Glu Met Asp Phe Ser Lys Met Lys Pro Leu Glu Ile Lys Glu 
                485                 490                 495     


Thr Val Ala Pro Ile Phe Glu Thr Leu Asn Ile Asn Thr Val Ile Leu 
            500                 505                 510         


Asp Phe Asp Pro Ser Ile Gln Gly Asn Asp Phe Asp Lys Gln Pro Tyr 
        515                 520                 525             


Tyr Gln Leu Trp His Leu Leu Tyr Ser Ala Glu Asp Asp Asp Lys Leu 
    530                 535                 540                 


Lys Glu Thr Leu Lys Thr Lys Phe Gly Phe Lys Glu Asn His Leu Pro 
545                 550                 555                 560 


Phe Leu Leu Asn Ile Asn Leu Gln Ala Asp Tyr Gly Ser Leu Ser Ala 
                565                 570                 575     


Lys Ala Ile Lys Lys Ile Leu Pro His Leu Met Asp Gly His Ile Tyr 
            580                 585                 590         


Asp Lys Ala Cys Leu Met Val Gly Tyr Asn His Ser Ser Ser Leu Thr 
        595                 600                 605             


Thr Ala Gln Asn Asn Asp Arg Val Leu Lys Asp Thr Leu Glu Leu Leu 
    610                 615                 620                 


Lys Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln 
625                 630                 635                 640 


Met Ile Asn Val Ile Asn Ala Ile Met Lys Asp Pro Ala Met Gly Lys 
                645                 650                 655     


Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Ala Asn Asn 
            660                 665                 670         


Glu Gln Arg Lys Lys Ala Thr Ser Asp Ile Asn Thr Ala Thr Thr Glu 
        675                 680                 685             


His Glu Arg Ile Arg Lys Leu Leu His Ser Glu Phe Asn Ile Pro Arg 
    690                 695                 700                 


Val Thr Arg Asn Asp Ile Ile Arg Tyr Lys Leu Trp Leu Glu Thr Gly 
705                 710                 715                 720 


Gly Ile Ser Leu Tyr Thr Gly Asn Pro Ile Lys Gly Ser Asp Leu Phe 
                725                 730                 735     


Ser Lys Asp Tyr Asp Ile Glu His Ile Ile Pro Lys Ser Arg Leu Phe 
            740                 745                 750         


Asp Asp Ser Phe Ser Asn Lys Thr Leu Cys Glu Arg Gln Leu Asn Ile 
        755                 760                 765             


Asp Lys Ser Asn Lys Thr Ala Tyr Ser Phe Leu Gln Glu Lys Leu Ser 
    770                 775                 780                 


Glu Asn Asp Phe Ala Ala Tyr Glu Asn Arg Val Lys Asp Leu Phe Lys 
785                 790                 795                 800 


Thr Gly Lys Ile Lys Ser Tyr Thr Lys Tyr Lys Lys Leu Leu Met Ala 
                805                 810                 815     


Asp Asn Glu Ile Pro Glu Gly Phe Ile Asp Arg Gln Leu Arg Glu Thr 
            820                 825                 830         


Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Leu Glu Val Ser Arg 
        835                 840                 845             


Asn Val Thr Ala Thr Ile Gly Ser Val Thr Asp Lys Leu Arg Glu Asp 
    850                 855                 860                 


Trp Glu Leu Val Asp Val Met Lys Glu Leu Asn Trp Glu Lys Tyr Asp 
865                 870                 875                 880 


Lys Leu Gly Leu Thr His Ile Glu Glu Gly Lys Asn Gly Glu Arg Leu 
                885                 890                 895     


Ser Lys Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala 
            900                 905                 910         


Met Asp Ala Ile Thr Val Ala Phe Thr Lys Pro Ala Tyr Ile Gln Tyr 
        915                 920                 925             


Leu Asn Asn Leu Asn Ala Lys Thr Gln Gly Glu Asn Lys Ala Asn Ser 
    930                 935                 940                 


Ile Ile Gly Ile Glu Asn Lys His Leu Tyr Arg Asp Lys Asn Asn Lys 
945                 950                 955                 960 


Leu Arg Phe Ile Ser Pro Met Lys Asn Phe Arg Glu Glu Ala Lys Lys 
                965                 970                 975     


Gln Leu Glu Ser Ile Leu Ile Ser Tyr Lys Ala Lys Asn Lys Val Val 
            980                 985                 990         


Thr Lys Asn Lys Asn Thr Thr Lys  Lys Ser Gly Gly Thr  Asn Gln Lys 
        995                 1000                 1005             


Ile Gln  Leu Thr Pro Arg Gly  Arg Leu His Lys Glu  Thr Val Tyr 
    1010                 1015                 1020             


Gly Lys  Leu Gln Gln Tyr Ala  Thr Lys Glu Glu Lys  Val Asn Ala 
    1025                 1030                 1035             


Ser Phe  Thr Glu Asp Tyr Ile  Gln Lys Val Ala Lys  Lys Glu Tyr 
    1040                 1045                 1050             


Arg Glu  Ala Leu Leu Lys Arg  Leu Glu Glu Asn Asp  Asn Asp Pro 
    1055                 1060                 1065             


Lys Lys  Ala Phe Thr Gly Lys  Asn Ala Leu Asn Lys  Thr Pro Ile 
    1070                 1075                 1080             


Tyr Ile  Ser Leu Lys Asp Asn  Ile Ile Val Pro Glu  Lys Val Lys 
    1085                 1090                 1095             


Thr Val  Trp Leu Glu Thr Asp  Tyr Thr Ile Arg Lys  Asp Ile Thr 
    1100                 1105                 1110             


Pro Asp  Leu Lys Ile Asp Lys  Val Ile Asp Val Gly  Ile Lys Arg 
    1115                 1120                 1125             


Ile Leu  Gln Asn Arg Leu Asn  Glu Phe Asn Gly Asp  Pro Lys Lys 
    1130                 1135                 1140             


Ala Phe  Val Asn Leu Gly Glu  Ser Pro Ile Trp Leu  Asn Lys Glu 
    1145                 1150                 1155             


Lys Gly  Ile Ala Ile Lys Arg  Val Thr Ile Ser Gly  Val Ser Asn 
    1160                 1165                 1170             


Ala Gln  Ala Leu His Thr Lys  Lys Asp His Leu Gly  Asn Glu Ile 
    1175                 1180                 1185             


Leu Asp  Lys Asn Gly Asn Pro  Ile Pro Val Asp Phe  Ile Ser Thr 
    1190                 1195                 1200             


Gly Asn  Asn His His Val Ala  Ile Tyr Arg Asp Glu  Lys Gly Asn 
    1205                 1210                 1215             


Leu Gln  Glu Glu Val Val Ser  Phe Tyr Asp Ala Val  Ile Arg Arg 
    1220                 1225                 1230             


Asn Leu  Glu Leu Ser Val Ile  Asn Lys Asn His Glu  Lys Gly Trp 
    1235                 1240                 1245             


Glu Phe  Leu Phe Ser Met Lys  Gln Asn Glu Phe Phe  Ile Phe Pro 
    1250                 1255                 1260             


Ser Asp  Gly Phe Asn Pro His  Glu Ile Asp Leu Leu  Asn Pro Asn 
    1265                 1270                 1275             


Asn Tyr  His Leu Ile Ser Pro  Asn Ile Phe Arg Val  Gln Lys Ile 
    1280                 1285                 1290             


Ser Thr  Lys Asn Tyr Met Phe  Asn His His Leu Glu  Thr Lys Ala 
    1295                 1300                 1305             


Val Asp  Gly Glu Met Leu Lys  Ser Lys Lys Glu Leu  Ser Lys Thr 
    1310                 1315                 1320             


Ser Tyr  His Phe Ile Gln Thr  Pro Thr Asn Leu Asn  Gly Ile Ile 
    1325                 1330                 1335             


Lys Val  Arg Ile Asn His Leu  Gly Lys Ile Val His  Leu Gly Glu 
    1340                 1345                 1350             


Tyr 
    


<210>  930
<211>  1354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  930

Met Ala Asp Phe Ala Lys Tyr Gln Pro Gly Met Val Ala Asp Gly Arg 
1               5                   10                  15      


Lys Ile Pro Leu Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu 
            20                  25                  30          


Thr Gln Ala Ile Arg Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe 
        35                  40                  45              


Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu 
    50                  55                  60                  


Asn Ser Thr Lys His Glu Glu Tyr Tyr Glu Leu Lys Val Val Ser Val 
65                  70                  75                  80  


Glu Ala Ala Glu Glu Gly Lys Gly Gly Asn Thr Trp Tyr Asn Val Thr 
                85                  90                  95      


Leu Glu Asn Gly Trp Ile Tyr Arg Arg Gln Ser Lys Ile Glu Leu Asn 
            100                 105                 110         


Asp Trp Val Gly Lys Val Arg Pro Phe Ile Val Thr Thr Glu Tyr Glu 
        115                 120                 125             


Ile Asp Gly Val Thr Ile Lys Lys Asp Lys Asn Gly Glu Val Lys Arg 
    130                 135                 140                 


Ser Phe Arg Ala Pro Ser Glu Asn Asp Trp Gly Leu Arg Lys Lys Arg 
145                 150                 155                 160 


Thr Glu Ser Leu Leu Glu Glu Ser Gly Lys Thr Val Gly Ala Phe Ile 
                165                 170                 175     


Tyr Asn His Ile Leu Ser Glu Pro Ala Asp Lys Ile Arg Gly Asn Phe 
            180                 185                 190         


Ile Arg Thr Ile Glu Arg Lys Phe Tyr Lys Lys Glu Leu Glu Ala Ile 
        195                 200                 205             


Leu Arg Glu Gln Ser Lys His Gln Lys Glu Leu Lys Asp Ile Asp Thr 
    210                 215                 220                 


Leu Ile Ala Cys Ile Lys Glu Leu Tyr Pro Asn Asn Lys Pro His Gln 
225                 230                 235                 240 


Asp Ser Leu Ile Lys Lys Asp Met Thr Tyr Leu Leu Ile Thr Asp Leu 
                245                 250                 255     


Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asp 
            260                 265                 270         


Cys Pro Tyr Glu Tyr Tyr Glu Tyr Val Ile Lys Glu Thr Gly Glu Asn 
        275                 280                 285             


Lys Arg Gln Ser Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Tyr Gln 
    290                 295                 300                 


Glu Phe Arg Leu Trp Gln Phe Ile Ile Asn Leu Arg Leu Phe Asp Arg 
305                 310                 315                 320 


Asn Asn Asp Lys Glu Val Thr Ser Glu Tyr Leu Ser Ser Met Glu Asp 
                325                 330                 335     


Tyr Thr Arg Leu Phe Thr Tyr Leu Asn Asp Arg Lys Asp Ile Asn Gln 
            340                 345                 350         


Glu Thr Leu Leu Lys Asp Phe Phe Lys Gln Lys Lys Val Lys Arg Gly 
        355                 360                 365             


Met Glu Lys Glu Phe Pro Ile Arg Trp Asn Tyr Ile Glu Asp Lys Glu 
    370                 375                 380                 


Lys Lys Tyr Pro Cys Asn Glu Thr Arg His Glu Leu Leu Thr Ala Leu 
385                 390                 395                 400 


Gly Lys Val Gly Ile Asp His Ser Trp Leu Asp Asp Lys Glu Leu Phe 
                405                 410                 415     


Tyr Arg Leu Trp His Leu Leu Tyr Ser Val Glu Ser Lys Glu Glu Ala 
            420                 425                 430         


Glu Gly Ala Leu Arg Lys Leu Val Asp Asn Asp Asp Phe Val Asn Ala 
        435                 440                 445             


Phe Leu Lys Ile Lys Pro Phe Lys Lys Glu Tyr Gly Ala Tyr Ser Glu 
    450                 455                 460                 


Lys Ala Ile Lys Lys Leu Leu Ala Val Met Arg Met Gly Asn Leu Trp 
465                 470                 475                 480 


Asn Glu Val Asp Ile Cys Glu Gln Thr Arg Asp Arg Ile Gln Asn Ile 
                485                 490                 495     


Ile Lys Gly Asp Ile Asp Glu Lys Ile Lys Asp Arg Met Gly Arg Thr 
            500                 505                 510         


Phe Ser Gln Leu Ser Asp Phe Gln Gly Leu Pro Glu Trp Leu Ala Cys 
        515                 520                 525             


Tyr Val Val Tyr Gly Gln His Ser Glu Ala Thr Asp Ile Gln Arg Trp 
    530                 535                 540                 


Thr Lys Pro Glu Asn Leu Met Ala Tyr Ile Asn Gly Phe Lys Gln His 
545                 550                 555                 560 


Ser Leu Arg Asn Pro Ile Val Glu Gln Cys Ile Leu Glu Thr Leu Arg 
                565                 570                 575     


Thr Val His Asp Ile Trp Lys Glu Ala Gly His Ile Asp Glu Ile His 
            580                 585                 590         


Ile Glu Leu Gly Arg Gly Met Lys Ser Thr Ala Asp Gln Arg Ala Arg 
        595                 600                 605             


Met Ser Lys Ser Ile Met Gln Asn Glu Asn Thr Asn Leu Arg Ile Lys 
    610                 615                 620                 


Ser Leu Leu Met Glu Leu Lys Asn Ala Lys Glu Phe Glu Asn Val Arg 
625                 630                 635                 640 


Pro Tyr Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly 
                645                 650                 655     


Ala Leu Gln Glu Leu Thr Lys Glu Asp Ala Gln Tyr Asn Glu Ile Ser 
            660                 665                 670         


Lys Ile Ser Gln Met Ala Gln Pro Ser Ala Asn Glu Leu Leu Lys Tyr 
        675                 680                 685             


Arg Leu Trp Leu Glu Gln Lys Tyr Cys Ser Pro Tyr Thr Gly Lys Pro 
    690                 695                 700                 


Ile Ser Leu Ser Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Ile 
705                 710                 715                 720 


Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Thr Asn Lys Val Ile 
                725                 730                 735     


Cys Glu Ala Glu Val Asn Gln Leu Lys Ser Asn Met Leu Gly Tyr Glu 
            740                 745                 750         


Phe Ile Lys Lys His Gly Gly Glu Ile Val Arg Cys Thr Met Ile Gly 
        755                 760                 765             


Asp Val Lys Leu Leu Asp Ala Lys Glu Tyr Tyr Asn Phe Val Thr Glu 
    770                 775                 780                 


His Tyr Thr Asn Asn Arg Arg Lys Lys Glu Lys Leu Leu Met Glu Asp 
785                 790                 795                 800 


Ile Pro Gln Glu Phe Leu Asn Arg Gln Met Asn Asp Ser Arg Tyr Ile 
                805                 810                 815     


Ser Lys Lys Val Met Ala Ile Leu Ser Asn Ile Val Arg Glu Glu Asp 
            820                 825                 830         


Glu Gln Glu Ala Lys Ser Lys Phe Val Ile Ala Cys Ser Gly Gly Ile 
        835                 840                 845             


Thr Asp Arg Leu Lys Gln Asp Trp Gly Leu Asn Asp Val Trp Asn Ser 
    850                 855                 860                 


Ile Val Tyr Pro Arg Phe Glu Arg Leu Asn Lys Leu Thr Asn Thr Glu 
865                 870                 875                 880 


Asn Phe Gly Arg Trp Glu Asn Lys Lys Gly Lys Gln Val Phe Gln Thr 
                885                 890                 895     


Ser Val Pro Leu Glu Leu Gln Lys Asp Phe Ser Lys Lys Arg Ile Asp 
            900                 905                 910         


His Arg His His Ala Met Asp Ala Leu Val Ile Ala Cys Ala Ser Asn 
        915                 920                 925             


Asn Ile Ile Asn Tyr Leu Asn Asn Glu Ser Ala Lys Ser Gln Glu Lys 
    930                 935                 940                 


Arg Glu Asp Leu Arg Lys Lys Leu Cys Asp Lys Asn Arg Ile Ile Arg 
945                 950                 955                 960 


Lys Pro Trp Glu Thr Phe Thr Gln Asp Ala His Thr Ala Leu Glu Asn 
                965                 970                 975     


Val Val Val Ser Phe Lys Asn Tyr Ile Arg Val Ile Asn Lys Ala Thr 
            980                 985                 990         


Asn Tyr Tyr Glu Lys Tyr Asp Lys  Asn Gly Lys Lys Glu  Ile Ala Glu 
        995                 1000                 1005             


Gln Lys  Gly Gln Asp Met Trp  Ala Ile Arg Lys Pro  Met His Lys 
    1010                 1015                 1020             


Glu Thr  Val Phe Gly Gln Val  Asn Leu Arg Arg Lys  Ile Thr Ile 
    1025                 1030                 1035             


Lys Leu  Lys Asp Ala Leu Glu  Lys Ile Pro Ala Ile  Cys Asp Lys 
    1040                 1045                 1050             


Glu Leu  Arg Asp Tyr Ile Asn  Ile Leu Val Ala Lys  Gln Phe Asn 
    1055                 1060                 1065             


Lys Lys  Gln Ile Leu Ala His  Phe Lys Ser Ile Asn  Tyr Arg Leu 
    1070                 1075                 1080             


Asn Met  Lys Ser Val Asp Lys  Val Asp Val Trp Gln  Phe Ser Asn 
    1085                 1090                 1095             


Glu Lys  Glu Pro Met Val Ala  Thr Arg Lys Ser Val  Asp Thr Ser 
    1100                 1105                 1110             


Phe Asp  Thr Lys Arg Ile Ala  Thr Ile Thr Asp Thr  Gly Ile Gln 
    1115                 1120                 1125             


Lys Ile  Leu Leu Asn Tyr Leu  Lys Ala Lys Asn Asn  Asp Pro Asn 
    1130                 1135                 1140             


Ile Ala  Phe Thr Pro Glu Gly  Ile Ala Glu Met Asn  Lys His Ile 
    1145                 1150                 1155             


Thr Glu  Tyr Asn Asn Gly Lys  Gln His Gln Pro Ile  Leu Lys Val 
    1160                 1165                 1170             


Arg Val  Ser Glu Pro Lys Gly  Ala Lys Tyr Gln Val  Gly Glu Thr 
    1175                 1180                 1185             


Gly Asn  Lys Arg Ser Lys Phe  Val Glu Ala Gln Lys  Gly Thr Asn 
    1190                 1195                 1200             


Leu Tyr  Phe Ala Ile Tyr Glu  Asp Lys Glu Gly Asn  Arg Ser Tyr 
    1205                 1210                 1215             


Asn Thr  Ile Pro Leu Asn Glu  Val Ile Glu Arg Leu  Lys Gln Gly 
    1220                 1225                 1230             


Leu Ser  Pro Val Pro Asp Ala  Asn Glu Lys Ser Ile  Pro Leu Lys 
    1235                 1240                 1245             


Phe His  Leu Ser Pro Asn Asp  Leu Val Tyr Val Pro  Lys Thr Asp 
    1250                 1255                 1260             


Glu Glu  Asn Glu Ile Lys Met  Gly Gln Ile Tyr Lys  Phe Val Asp 
    1265                 1270                 1275             


Ser Ser  Gly Thr Thr Ala Asn  Phe Val Pro His Arg  Val Ser Asn 
    1280                 1285                 1290             


Leu Ile  Tyr Asn Val Asp Lys  Lys Ile Ala Glu Asn  Phe Cys Asn 
    1295                 1300                 1305             


His Glu  Ile Ile Gln Asn Glu  Tyr Gly Ile Gly Ser  Pro Gln Ser 
    1310                 1315                 1320             


Lys Asn  Gln Lys Ser Ile Thr  Gly Glu Met Ile Lys  Ala Val Cys 
    1325                 1330                 1335             


Trp Lys  Leu Glu Val Asp Arg  Leu Gly Asn Ile His  Lys Ile Ile 
    1340                 1345                 1350             


Lys 
    


<210>  931
<211>  1354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  931

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ile Lys Glu Pro Glu Lys Asp Val Val Gly Ser Glu Ile Val 
            20                  25                  30          


Asp Met Gly Val Arg Ile Val Pro Leu Ser Ser Asp Glu Glu Asn Asp 
        35                  40                  45              


Phe Ala Lys Gly Asn Thr Ile Ser Ile Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Asn Leu Gln Arg Phe Lys Gln Arg Arg Asn Ala 
65                  70                  75                  80  


Leu Leu Glu Thr Phe Lys Glu Lys Asn Leu Ile Pro Ser Asn Phe Gln 
                85                  90                  95      


Tyr Ala Glu Asp Gly Pro Ser Ser Thr Phe Ser Thr Leu Lys Leu Arg 
            100                 105                 110         


Asp Lys Ala Ala Lys Glu Lys Ile Glu Leu Asp Asp Phe Val Lys Val 
        115                 120                 125             


Leu Leu Gln Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Met Ser Glu Glu Asp Glu Gly Thr Ala Ile Asp Ser Met Gly Ile Ala 
145                 150                 155                 160 


Lys Glu Leu Tyr Glu Asn Asp Leu Thr Pro Gly Gln Trp Val Tyr Glu 
                165                 170                 175     


Ala Leu Lys Asn Gly Arg Lys Asn Val Pro Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         


Leu Gln Glu Glu Phe Lys Lys Ile Val Asn Tyr Gln Ser Glu Phe Tyr 
        195                 200                 205             


Pro Asp Ile Leu Asn Thr Ser Phe Val Glu Asp Trp Met Gly Lys Ala 
    210                 215                 220                 


Ser Thr Pro Thr Lys Gln Tyr Phe Asn Lys Arg Gly Val Gln Leu Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Lys Arg Glu Glu Arg Arg Leu Gln Glu Tyr Lys Trp 
                245                 250                 255     


Arg Ala Glu Ala Val Asn Phe Lys Ile Asp Leu Ser Glu Ile Ala Leu 
            260                 265                 270         


Ile Leu Ser Gln Ile Asn Ser Gln Ile Ser Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Lys Asn Leu Thr 
    290                 295                 300                 


Val Gly Gln Tyr Leu Tyr Gln Gln Val Lys Lys Asn Pro His Thr Arg 
305                 310                 315                 320 


Leu Lys Gly Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Lys Ile Trp Ser Val Gln Ser Ser Phe Tyr Pro Gln Leu Asn Asp Glu 
            340                 345                 350         


Leu Lys Gln Glu Val Arg Asp Val Thr Ile Phe Phe Gln Arg Arg Leu 
        355                 360                 365             


Lys Ser Gln Lys His Leu Ile Ser Asn Cys Glu Phe Glu Glu His His 
    370                 375                 380                 


Lys Val Val Pro Lys Ser His Pro Ile Phe Gln Glu Phe Arg Ile Trp 
385                 390                 395                 400 


Gln Asn Leu Asn Asn Leu Thr Leu Ile Lys Lys Asp Asn Leu Asn Glu 
                405                 410                 415     


Lys Cys Glu Leu Ala Leu Glu Asp Lys Thr Ala Leu Ala Asn Glu Leu 
            420                 425                 430         


Ala Phe Lys Lys Glu Leu Ser Val Lys Asp Ala Leu Lys Val Leu Gly 
        435                 440                 445             


Leu Lys Pro Ser Asp Trp Glu Phe Asn Phe Thr Lys Ile Glu Gly Asn 
    450                 455                 460                 


Arg Thr Asn His Ala Phe Phe Glu Ala Phe Ala Lys Ile Ile Glu Leu 
465                 470                 475                 480 


Glu Asp Gly Glu Pro Ile Asp Leu Gly Asn Leu Lys Ala Asp Asp Ile 
                485                 490                 495     


Leu Asp Gln Phe Ser Glu Ala Phe Leu Arg Ile Gly Ile Asp Thr Glu 
            500                 505                 510         


Leu Leu Gln Val Asn Ser Asp Ile Glu Gly Ala Glu Tyr Glu Lys Gln 
        515                 520                 525             


Ser Tyr Ile Gln Phe Trp His Leu Leu Tyr Ser Ser Glu Asp Asp Gln 
    530                 535                 540                 


Lys Leu Lys Gln Asn Leu Ile Arg Lys Phe Gly Phe Lys Pro Glu His 
545                 550                 555                 560 


Ala Lys Ile Leu Ala Ser Ile Ser Leu Gln Asp Asp His Ala Ser Leu 
                565                 570                 575     


Ser Ser Arg Ala Ile Lys Lys Ile Leu Pro His Leu Gln Ser Gly Leu 
            580                 585                 590         


Ile Tyr Asp Lys Ala Cys Thr Tyr Ala Gly Tyr Asn His Ser Ser Ser 
        595                 600                 605             


Phe Thr Lys Asp Glu Asn Glu Lys Arg Glu Leu Arg Ala Glu Leu Glu 
    610                 615                 620                 


Leu Leu Lys Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu 
625                 630                 635                 640 


Asn Gln Met Ile Asn Val Val Asn Ala Ile Leu Lys Asp Pro Asn Leu 
                645                 650                 655     


Gly Arg Pro Asp Glu Ile Arg Val Glu Met Ala Arg Glu Leu Lys Ala 
            660                 665                 670         


Asn Ala Glu Gln Arg Lys Asn Met Thr Ser Asn Ile Ala Ser Ala Thr 
        675                 680                 685             


Arg Asp His Glu Lys Tyr Arg Glu Ile Leu Lys Lys Glu Phe Gly Leu 
    690                 695                 700                 


Lys Arg Val Thr Lys Asn Asp Leu Leu Arg Tyr Lys Leu Trp Leu Glu 
705                 710                 715                 720 


Thr Asp Gly Ile Ser Leu Tyr Thr Gly Lys Pro Ile Glu Ala Ser Lys 
                725                 730                 735     


Leu Phe Ser Lys Glu Tyr Asp Ile Glu His Ile Ile Pro Lys Ala Arg 
            740                 745                 750         


Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Ile Cys Glu Arg Gln Leu 
        755                 760                 765             


Asn Ile Asp Lys Ala Asn Val Thr Ala Phe Ser Phe Leu Gln Asn Lys 
    770                 775                 780                 


Leu Ser Ala Asp Glu Phe Glu Gln Tyr Gln Ser Arg Val Lys Ser Leu 
785                 790                 795                 800 


Tyr Gly Lys Leu Ser Lys Ala Lys Ile Gln Lys Leu Leu Met Ala Asn 
                805                 810                 815     


Asp Lys Ile Pro Glu Asp Phe Ile Ala Arg Gln Leu Gln Glu Thr Arg 
            820                 825                 830         


Tyr Ile Ser Lys Lys Ala Lys Glu Ile Leu Phe Glu Ile Thr Met Arg 
        835                 840                 845             


Val Ser Val Thr Thr Gly Thr Ile Thr Asp Lys Leu Arg Glu Asp Trp 
    850                 855                 860                 


Gly Leu Val Glu Ile Met Lys Glu Leu Asn Trp Glu Lys Tyr Asp Lys 
865                 870                 875                 880 


Leu Gly Leu Thr Tyr Thr Ile Glu Gly Lys His Gly Glu Arg Leu Asn 
                885                 890                 895     


Lys Ile Lys Asp Trp Asn Lys Arg Asn Asp His Arg His His Ala Met 
            900                 905                 910         


Asp Ala Leu Thr Val Ala Leu Thr Lys Pro Ala Tyr Ile Gln Tyr Leu 
        915                 920                 925             


Asn Asn Leu Asn Ala Lys Ala Ile Gly Asn Lys Lys Gly Thr Glu Ile 
    930                 935                 940                 


Phe Ala Ile Glu Gln Lys Tyr Leu Lys Arg Glu Asn Gly Lys Leu Cys 
945                 950                 955                 960 


Phe Ile Pro Pro Ile Glu Asn Ile Arg Ala Glu Ala Lys Lys His Leu 
                965                 970                 975     


Ser Lys Ile Leu Val Ser Tyr Lys Ala Lys Asn Lys Val Val Thr Ile 
            980                 985                 990         


Asn Lys Asn Lys Thr Lys Lys Lys  Ala Gly Phe Asn Glu  Gln Thr Ala 
        995                 1000                 1005             


Leu Thr  Pro Arg Gly Gln Leu  His Lys Glu Thr Val  Tyr Gly Lys 
    1010                 1015                 1020             


Ser Phe  His Tyr Ser Thr Lys  Phe Glu Lys Ile Gly  Ala Ser Phe 
    1025                 1030                 1035             


Asp Ala  Gln Lys Ile Asn Thr  Val Ala Lys Lys Glu  Glu Arg Glu 
    1040                 1045                 1050             


Ala Leu  Leu Lys Arg Leu Ala  Glu Asn Gly Asn Asp  Pro Lys Lys 
    1055                 1060                 1065             


Ala Phe  Thr Gly Lys Asn Ser  Leu Asn Lys Met Pro  Ile Tyr Leu 
    1070                 1075                 1080             


Asp Leu  Gly Lys Asn Ile Lys  Leu Ser Glu Lys Val  Lys Thr Val 
    1085                 1090                 1095             


Val Leu  Glu Gln Asn Tyr Thr  Ile Arg Lys Asn Ile  Asp Pro Asp 
    1100                 1105                 1110             


Leu Lys  Val Asp Lys Val Ile  Asp Val Gly Ile Lys  Arg Ile Leu 
    1115                 1120                 1125             


Glu Ser  Arg Leu Glu Glu Phe  Gly Gly Asn Ala Lys  Leu Ala Phe 
    1130                 1135                 1140             


Ser Asn  Leu Glu Glu Asn Pro  Ile Trp Leu Asn Lys  Glu Lys Gly 
    1145                 1150                 1155             


Ile Pro  Ile Lys Arg Val Lys  Ile Ser Gly Val Ser  Asn Val Glu 
    1160                 1165                 1170             


Ser Leu  His Val Lys Lys Asp  His Phe Gly Glu Lys  Ile Leu Asp 
    1175                 1180                 1185             


Glu Glu  Gly Asn Glu Ile Pro  Val Asp Phe Val Ser  Thr Gly Asn 
    1190                 1195                 1200             


Asn His  His Val Ala Ile Tyr  Glu Asp Glu Asp Gly  Asn Leu Gln 
    1205                 1210                 1215             


Asp Glu  Val Val Asn Phe Tyr  Glu Ala Val Ile Arg  Arg Asn Gln 
    1220                 1225                 1230             


Gly Glu  Ser Ile Ile Lys Tyr  Arg His Glu Glu Gly  Trp Asn Phe 
    1235                 1240                 1245             


Leu Tyr  Ser Ile Lys Gln Asn  Glu Phe Phe Val Phe  Pro Ser Gln 
    1250                 1255                 1260             


Asp Phe  Asn Pro Asn Asp Phe  Asp Leu Leu Asp Pro  Lys Asn Tyr 
    1265                 1270                 1275             


Asp Leu  Ile Ser Pro Asn Leu  Tyr Arg Ala Gln Lys  Phe Ser Lys 
    1280                 1285                 1290             


Val Met  Tyr Gly Asn Ser Ala  Val Arg Asp Tyr Val  Phe Arg His 
    1295                 1300                 1305             


His Leu  Glu Thr Val Leu Asn  Asp Val Lys Glu Leu  Lys Asp Val 
    1310                 1315                 1320             


Ala Tyr  Lys Gln Leu Lys Ser  Leu Gln Tyr Leu Asn  Gly Ile Lys 
    1325                 1330                 1335             


Lys Val  Arg Leu Asn His Leu  Gly Gln Ile Ile Gln  Val Gly Glu 
    1340                 1345                 1350             


Tyr 
    


<210>  932
<211>  1354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  932

Met Thr Leu His Tyr Asn Val Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Tyr Thr Ile Val Asp Asp Asn Gly Lys Ile Leu Gln Val Lys Gly 
            20                  25                  30          


Lys Asn Gly Tyr Gly Val Arg Val Phe Lys Glu Gly Ala Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Ile Phe Arg Thr Thr His Arg Arg Leu Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Gln Asp Phe Phe Glu Pro Tyr Ile Leu Pro 
65                  70                  75                  80  


Gln Asp Asp Gly Phe Phe Ile Arg Arg Lys Glu Ser Asn Leu Val Leu 
                85                  90                  95      


Asn Asp Arg Asp Glu Asp Thr Ala Ser Leu Phe Asn Asp Arg Ser Asp 
            100                 105                 110         


Arg Asp Phe Tyr Gln Ala Tyr Pro Thr Ile Tyr His Leu Arg Gln Ala 
        115                 120                 125             


Leu Met Thr Glu Lys Arg Gln Phe Asp Val Arg Glu Ile Tyr Leu Ala 
    130                 135                 140                 


Met His His Ile Ile Lys Tyr Arg Gly His Phe Leu Leu Asn Gly Thr 
145                 150                 155                 160 


Val Asn Asn Phe Thr Asn Asn Lys Ile Asp Leu His Ser Asp Phe Glu 
                165                 170                 175     


Glu Leu Asn Ala Leu Leu Leu Asn Val Ile Pro Glu Leu Lys Phe Gln 
            180                 185                 190         


Ile Asp Leu Asp His Ser Thr Met Phe Gln Thr Thr Leu Leu Asn Thr 
        195                 200                 205             


Thr Ile Ser Arg Ser Gly Arg Gln Arg Asp Leu Val Lys Ser Phe Tyr 
    210                 215                 220                 


Asn Gly Ser Asp Asp Lys Gln Leu Ala Lys Gln Gln Lys Ser Val Ala 
225                 230                 235                 240 


Thr Glu Leu Leu Lys Ala Ile Leu Gly Leu Lys Ala Lys Phe His Lys 
                245                 250                 255     


Leu Phe Asn Leu Asp Leu Leu Glu Thr Thr Glu Trp Glu Phe Ser Phe 
            260                 265                 270         


Asp Ala Glu Asp Ile Asp Asp Lys Leu Ala Lys Leu Glu Pro Leu Met 
        275                 280                 285             


Thr Glu Asn Gln Gln Gln Val Met Asp Ile Leu Gln Arg Ile Phe Ala 
    290                 295                 300                 


Ala Ile Thr Leu Asn Gly Ile Val Pro Glu Gly Lys Thr Leu Ser Ala 
305                 310                 315                 320 


Ser Lys Val Asp Ser Tyr Gln Lys His Lys Gln Asp Leu Lys Leu Leu 
                325                 330                 335     


Lys Ala Ile Ala Gln His Ser Asp Lys Lys Thr Ala Thr Val Leu Gln 
            340                 345                 350         


Thr Ala Tyr Asn Glu Tyr Ile Asn Gly Ile Ala Ser Lys Pro Leu Lys 
        355                 360                 365             


Gln Glu Asp Phe Tyr Lys Arg Leu Thr Asn Ala Val Lys Lys Ser Ser 
    370                 375                 380                 


Asp Pro Gln Val Ala Asp Met Leu Ala Leu Ile Asp Gln Glu Lys Phe 
385                 390                 395                 400 


Leu Pro Lys Gln Arg Thr Lys Glu Asn Gly Ala Ile Pro His Gln Leu 
                405                 410                 415     


His Gln Leu Glu Leu Glu Arg Ile Ile Asp Asn Gln Lys Asp Tyr Tyr 
            420                 425                 430         


Pro Trp Leu Ala Glu Leu Asn Pro Asn Gln Ala Arg Gln Lys Val Ala 
        435                 440                 445             


Lys Tyr Lys Leu Ser Glu Leu Val Ala Phe Arg Val Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Leu Ile Glu Pro Glu Val Gln Gln Ala Thr Ser Asn Ala His 
465                 470                 475                 480 


Phe Ala Trp Met Ala Arg Lys Glu Ala Gly Pro Ile Thr Pro Trp Asn 
                485                 490                 495     


Phe Asp Gln Lys Val Asp Arg Asn Val Ser Ala Glu Arg Phe Ile Lys 
            500                 505                 510         


Arg Met Thr Thr Lys Asp Thr Tyr Leu Leu Ala Glu Asp Val Leu Pro 
        515                 520                 525             


Leu His Ser Leu Leu Tyr Gln Arg Phe Ile Val Leu Asn Glu Leu Asn 
    530                 535                 540                 


Asn Val Arg Val Asn Gly Gln Lys Leu Thr Lys Lys Gln Lys Gln Ala 
545                 550                 555                 560 


Val Tyr Gln Asp Leu Phe Lys Arg Gln Pro His Val Thr Lys Lys Gln 
                565                 570                 575     


Phe Lys Ser Tyr Leu Val Gln Thr Gly Glu Phe Ala Glu Ser Ser Lys 
            580                 585                 590         


Ile Glu Gly Leu Ala Asn Glu Thr Ser Phe Asn Ser Gly Leu Thr Thr 
        595                 600                 605             


Glu Asn Glu Leu Arg Lys Ile Phe Gly Ala Gln Leu Asp Glu Ala Arg 
    610                 615                 620                 


Tyr Gln Val Asp Phe Glu Gln Ile Ile Glu Trp Ala Thr Leu Phe Glu 
625                 630                 635                 640 


Asp Ala Lys Ile Leu Arg Thr Lys Leu Ala Glu Ile Thr Trp Leu Thr 
                645                 650                 655     


Thr Glu Gln Ile Glu Lys Leu Ala Gly Ile Arg Tyr Arg Gly Trp Gly 
            660                 665                 670         


Arg Phe Ser Arg Lys Leu Leu Ala Gly Leu Arg Asp Gln Asn Gly Gln 
        675                 680                 685             


Gln Ile Ile Asp Leu Leu Trp Asp Thr Pro Asn Asn Phe Met Val Ile 
    690                 695                 700                 


Val Ser Gln Ala Ala Phe Ser Glu Ala Ile Thr Lys Glu Asn Glu Lys 
705                 710                 715                 720 


Leu Ile Asp Arg Arg Gly Ala Gln Asp Val Ile Thr Asp Leu Tyr Thr 
                725                 730                 735     


Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Val Leu Ala Ile Val Ala 
            740                 745                 750         


Asp Val Gln Lys Ala Met Gly Gly Val Pro Pro Gln Arg Ile Phe Ile 
        755                 760                 765             


Glu Phe Ala Arg Glu Asp Glu Lys Asn Pro Arg Arg Ser Val Glu Arg 
    770                 775                 780                 


Ser Arg Gln Leu Glu Lys Leu Tyr Gln Thr Ile Ser Asn Glu Phe Leu 
785                 790                 795                 800 


Ile Asn Ser Glu Val Arg Gln Glu Leu Lys Glu Ala Val Asp Gln Lys 
                805                 810                 815     


Val Asn Phe Lys Asp Arg Leu Phe Leu Tyr Phe Leu Gln Gly Gly Val 
            820                 825                 830         


Asp Leu Tyr Ser Gly Lys Arg Ile Asn Ile Asp Gln Leu Ser His Tyr 
        835                 840                 845             


Asp Ile Asp His Ile Leu Pro Gln Ser Phe Ile Lys Asp Asp Ser Leu 
    850                 855                 860                 


Asp Asn Arg Val Leu Val Ser Gln Lys Leu Asn Arg Ser Lys Ser Asp 
865                 870                 875                 880 


Ser Val Pro Leu Ser Glu Phe Ala Asn Tyr Lys Phe Gly Pro Ala Met 
                885                 890                 895     


Gln Ala Lys Trp Leu Gln Leu Lys Glu Ala Gly Leu Leu Ser Lys Arg 
            900                 905                 910         


Lys Tyr Asp Asn Leu Thr Leu Asp Pro Thr Lys Leu Ser Lys Phe Ala 
        915                 920                 925             


Pro Leu Gly Phe Val Asn Arg Gln Leu Val Glu Thr Arg Gln Val Ile 
    930                 935                 940                 


Lys Leu Ala Ala Asn Leu Leu Ala Ser Glu Glu Thr Lys Val Val Thr 
945                 950                 955                 960 


Ile Lys Ala Asn Met Thr His Gln Val Arg Lys Glu Leu Asp Phe Pro 
                965                 970                 975     


Lys Asn Arg Asn Val Asn Asn Tyr His His Ala Phe Asp Ala Tyr Leu 
            980                 985                 990         


Thr Ala Phe Val Gly Ile Phe Leu  Ser Lys Arg Tyr Pro  Lys Leu Lys 
        995                 1000                 1005             


Pro Tyr  Phe Thr Tyr Gly Asp  Phe Gln Lys Gly Asn  Lys Leu Pro 
    1010                 1015                 1020             


Asp Leu  Lys Asn Phe Asn Phe  Leu Tyr Glu Leu Lys  Asn Lys Asp 
    1025                 1030                 1035             


Arg Ser  Ile Asp Ser Asn Thr  Gly Glu Ile Ile Trp  Asp Lys Gln 
    1040                 1045                 1050             


Arg Asp  Leu Ala Tyr Met Asn  Lys Ile Tyr Asn Phe  Lys Lys Val 
    1055                 1060                 1065             


Thr Val  Val His Glu Val Leu  Thr Lys Ser Gly Ala  Leu Tyr Asn 
    1070                 1075                 1080             


Gln Thr  Leu Tyr Lys Ala Ser  Glu Asp Lys Ala Ser  Gly Arg Gly 
    1085                 1090                 1095             


Thr Lys  Gln Leu Ile Arg Lys  Lys Asp Asn Met Pro  Thr Glu Leu 
    1100                 1105                 1110             


Tyr Gly  Gly Tyr Thr Gly Ser  Thr Ser Ala Phe Met  Ser Ile Val 
    1115                 1120                 1125             


Arg Leu  Trp Lys Lys Asp Lys  Pro Tyr Tyr Lys Val  Val Gly Ile 
    1130                 1135                 1140             


Pro Thr  Arg Met Ala Ala Lys  Leu Ala Asn Gln Ala  Gln Leu Leu 
    1145                 1150                 1155             


Asp Tyr  Leu Thr Lys Lys Phe  Thr Thr Arg Lys Leu  Val Lys Lys 
    1160                 1165                 1170             


Thr Gly  Asp Tyr Lys Thr Thr  Val Glu Arg Phe Glu  Leu Val Val 
    1175                 1180                 1185             


Pro Lys  Val Gly Phe Asn Gln  Leu Val Ile Asp Gly  Gly Gln Pro 
    1190                 1195                 1200             


Phe Met  Leu Gly Ser Ala Thr  Tyr Gln Tyr Asn Ala  Arg Glu Leu 
    1205                 1210                 1215             


Phe Ile  Ser Lys Glu Ala Val  Lys Ala Leu Asn Lys  Gln Leu Ser 
    1220                 1225                 1230             


Thr His  Val Asp Leu Val Gln  Val Phe Asp Glu Ile  Leu Val Gln 
    1235                 1240                 1245             


Val Asn  Arg Trp Phe Pro Leu  Tyr Asp Thr Asn Gly  Phe Arg Glu 
    1250                 1255                 1260             


Lys Leu  Ser His Gly Arg Asp  Arg Phe Met Lys Leu  Asn Asp Ser 
    1265                 1270                 1275             


Phe Glu  Glu Lys Ser Asp Thr  Gln Ile Asp Val Leu  Asn Arg Ile 
    1280                 1285                 1290             


Leu Ile  Gly Phe His Ala Asn  Ala Ala Arg Thr Asn  Leu Lys Ile 
    1295                 1300                 1305             


Leu Gly  Leu Gly Thr Asp Leu  Gly Phe Met Thr Gln  Gln Ala Gly 
    1310                 1315                 1320             


Ile Arg  Leu Thr Glu Lys Ala  Ile Leu Val His Gln  Ser Pro Ser 
    1325                 1330                 1335             


Gly Leu  Phe Glu Arg Lys Val  Ala Leu Arg Asp Leu  Lys Gly Leu 
    1340                 1345                 1350             


Arg 
    


<210>  933
<211>  1355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  933

Met Thr Tyr Ser Ile Gly Leu Asp Ile Gly Thr Gly Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Gly Asp Asp Tyr Arg Leu Lys Arg Ala Lys Gly Lys Asn 
            20                  25                  30          


Leu Ile Gly Val Arg Leu Phe Asp Ser Ala Thr Thr Ala Ala Glu Arg 
        35                  40                  45              


Arg Gly Tyr Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg His Trp Arg 
    50                  55                  60                  


Leu Arg Leu Leu Asn Asp Ile Phe Ala Ser Asp Leu Ala Glu Gln Asp 
65                  70                  75                  80  


Glu Asn Phe Leu Pro Arg Leu Lys Tyr Ser Trp Val Asn Pro Asp Asp 
                85                  90                  95      


Glu Gln Asn Pro Gln Leu Asn Ser Asp Val Ala Asn Gly Ala Leu Phe 
            100                 105                 110         


Gly Ser Ser Glu Thr Asp Lys Ala Phe His Lys Asn Tyr Pro Thr Ile 
        115                 120                 125             


Tyr His Leu Arg His Glu Leu Met Thr Asp Thr Ala Lys His Asp Leu 
    130                 135                 140                 


Arg Glu Val Tyr Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn 
145                 150                 155                 160 


Phe Leu Asn Thr Ala Asp Lys Ile Asn Ala Glu Asn Ser Phe Asn Val 
                165                 170                 175     


Asp Leu Phe Ile Ala Ala Leu Asp Glu Tyr Asn Glu Phe Leu Glu Thr 
            180                 185                 190         


Pro Ile Thr Ser Ile Lys Asp Ala Gln Leu Phe Ser Asp Lys Leu Thr 
        195                 200                 205             


Asp Ile Asn Ala Arg Asn Lys Ser Val Arg Val Glu Asn Ala Met Ser 
    210                 215                 220                 


Glu Thr Tyr Asp Asp Lys Ser Lys Ile Phe Lys Ala Ile Leu Thr Ala 
225                 230                 235                 240 


Leu Val Gly Asn Thr Ala Asn Phe Gln Thr Ile Phe Asp Leu Ser Asp 
                245                 250                 255     


Leu Glu Lys Asp Glu Ala Lys Asn Phe Lys Phe Lys Leu Asp Leu Glu 
            260                 265                 270         


Asp Val Glu Thr Lys Leu Asp Val Val Arg Ala Lys Leu Ser Asp Glu 
        275                 280                 285             


Gln Asn Ala Phe Leu Glu Ala Val Leu Ile Ala Tyr Asp Gly Ile Thr 
    290                 295                 300                 


Leu Lys Met Ile Leu Gly Asp Tyr Lys Ser Ile Ser Gln Ser Met Ile 
305                 310                 315                 320 


Glu Ser Tyr Lys Ser His Gln Arg Asp Trp Lys Tyr Ile Lys Asn Asn 
                325                 330                 335     


Val Arg Leu Asn Glu Gln Lys Ile Glu Pro Asp Lys Asn Ser Val Lys 
            340                 345                 350         


Phe Phe Lys Leu Asp Pro Lys Lys Leu Ile Tyr Pro Ser Asn Leu Ala 
        355                 360                 365             


Tyr Leu Gln Val Ile Ser Ser Asn Glu Asp Glu His Arg Lys Gly Leu 
    370                 375                 380                 


Asp Tyr Phe Lys Thr Ala Ile Gln Asp Ala Asp Ile Leu Gln Arg Ile 
385                 390                 395                 400 


Glu Asn Asp Thr Phe Leu Pro Arg Gln Arg Thr Lys Arg Asn Gly Val 
                405                 410                 415     


Leu Pro Tyr Gln Leu His Leu Ala Glu Leu Glu Gln Ile Ile Lys Lys 
            420                 425                 430         


Gln Ser Lys Tyr Tyr Pro Phe Leu Ala Asn Ile Tyr Thr Gln Asp Asn 
        435                 440                 445             


Lys Thr Glu Asn Lys Leu Ile Gly Leu Leu Lys Phe Arg Val Pro Tyr 
    450                 455                 460                 


Tyr Val Gly Pro Leu Val Glu Ala Glu Lys Val Thr Gly Asp Gly Lys 
465                 470                 475                 480 


Asn His Trp Leu Ile Lys Lys Glu Leu Gln Glu Asn Thr Pro Ile Thr 
                485                 490                 495     


Pro Phe Asn Phe Asp Asp Val Val Asp Lys Asp Lys Ser Gly Glu Gln 
            500                 505                 510         


Phe Ile Lys Arg Ile Thr Gly Thr Asp Ser Tyr Leu Ile Gly Glu Pro 
        515                 520                 525             


Thr Leu Pro Glu Asn Ser Leu Leu Tyr Gln Lys Tyr Asn Val Leu Gln 
    530                 535                 540                 


Glu Leu Asn Asn Val Arg Asn Glu Arg His Gln Arg Leu Pro Ile Lys 
545                 550                 555                 560 


Val Lys Gln Asp Ile Phe Glu His Val Phe Lys Ile Asp Lys Asn Val 
                565                 570                 575     


Asn Ala Lys Lys Val Met Ala Tyr Leu Lys Leu Asn Gly Tyr Gly Asp 
            580                 585                 590         


Phe Gln Ile Ala Gly Leu Ser Asp Thr Arg Thr Gly Lys Phe Asn Ser 
        595                 600                 605             


Asn Leu Ser Ser Tyr Asn Tyr Phe Val Asp Leu Leu Gly Arg Glu Lys 
    610                 615                 620                 


Thr Glu Lys Ile Arg Glu Asp Arg Leu Glu Glu Ile Ile Glu Ile Gln 
625                 630                 635                 640 


Thr Val Phe Glu Asp Lys Ile Leu Val Ala Lys Arg Leu Ser Asn Leu 
                645                 650                 655     


Asp Phe Leu Thr Pro Gln Glu Lys Ser Lys Leu Ser Ala Lys His Tyr 
            660                 665                 670         


Thr Gly Trp Gly Arg Leu Ser Gln Lys Leu Leu Thr Thr Gln Phe Ile 
        675                 680                 685             


Lys Thr Ala Leu Ser Glu Ala Gly Asp Ile Ile Pro Gln Ser His Ser 
    690                 695                 700                 


Ile Ile Asp Leu Leu Tyr Tyr Thr Asp Lys Asn Leu Met Glu Ile Leu 
705                 710                 715                 720 


Asn Asp Lys Thr Phe Asn Val Arg Asn Trp Leu Thr Glu Gln Asn Lys 
                725                 730                 735     


Gly Thr Glu Ser Gly Asn Ser Leu Tyr Asp Gln Ile Asp Ala Leu Ala 
            740                 745                 750         


Gly Pro Lys Asp Ile Lys Arg Gly Ile Thr Gln Val Phe Ser Ile Ile 
        755                 760                 765             


Asp Asp Ile Lys Lys Ala Met Gly Ser Asn Pro Glu Thr Val Tyr Leu 
    770                 775                 780                 


Glu Phe Ala Arg Glu Thr Gln Thr Ser Arg Leu Thr Asn Ser Arg Leu 
785                 790                 795                 800 


Asn Arg Val Gln Glu Ile Tyr Asn Ser Pro Glu Leu Lys Thr Glu Phe 
                805                 810                 815     


Ala Tyr Leu Ala Lys Lys Leu Glu Ala Glu Thr Lys Glu Ser Ile His 
            820                 825                 830         


Asp Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Glu Gly Arg Asp Met Tyr 
        835                 840                 845             


Thr Gly Lys Glu Ile Pro Phe Asp Gln Ile Ser Ser Asn Tyr Asp Ile 
    850                 855                 860                 


Asp His Ile Ile Pro Gln Ala Phe Thr Lys Asp Asp Ser Leu Asp Asn 
865                 870                 875                 880 


Arg Val Leu Val Asn Arg Ala Thr Asn Ala Arg Lys Ser Asn Ser Pro 
                885                 890                 895     


Thr Phe Thr Asn Asp Ile Ile Asp Asn Arg Lys Ser Trp Trp Gln Ser 
            900                 905                 910         


Leu Val Lys Arg Gly Leu Met Ser Lys Glu Lys Phe Glu Arg Leu Thr 
        915                 920                 925             


Asp Lys Arg Lys Thr Phe Ser Glu Asn Gln Thr Asn His Phe Ile Ala 
    930                 935                 940                 


Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys Asn Val Ala Asp Leu 
945                 950                 955                 960 


Ile Lys Asn Asn Tyr Asp Gly Thr Gln Ala Val Ala Ile Arg Ala Ser 
                965                 970                 975     


Leu Thr Gly Glu Met Arg Arg Tyr Leu Asp Trp Pro Lys Asn Arg Asp 
            980                 985                 990         


Met Asn Asp Tyr His His Ala His  Asp Ala Leu Met Met  Ala Thr Val 
        995                 1000                 1005             


Gly Lys  Tyr Val Asp Arg Arg  Gly Phe Phe Asp Asn  Gly Ser Val 
    1010                 1015                 1020             


Ser Asp  Gly Ala Gly Asn Ala  Tyr Asn Leu Tyr Thr  Lys Asp Trp 
    1025                 1030                 1035             


Leu Val  Ala Ala Arg Val Gly  Thr Arg Asn Asp Ser  Gln Arg Val 
    1040                 1045                 1050             


Asn Pro  Tyr Gly Phe Ile Val  Gly Ser Met Asn Ser  Lys Ala Val 
    1055                 1060                 1065             


Arg Gln  Gln Val Asn Lys Glu  Thr Gly Glu Ile Val  Trp Gly Asn 
    1070                 1075                 1080             


Glu Asn  Leu Asp Tyr Leu Arg  His Val Leu Ser Tyr  Lys Lys Ile 
    1085                 1090                 1095             


Leu Val  Thr Arg Arg Leu Ser  Gly Ser Ser Gly Arg  Leu Tyr Ala 
    1100                 1105                 1110             


Glu Thr  Leu Phe Ser Pro Asn  Pro Lys Ala Lys Leu  Ile Ala Gln 
    1115                 1120                 1125             


Lys Ala  Asn Arg Ser Val Ala  Leu Tyr Gly Gly Phe  Thr Lys Ala 
    1130                 1135                 1140             


Glu Ser  Ala Tyr Thr Ala Leu  Val Glu Phe Asn Lys  Lys Gly Asp 
    1145                 1150                 1155             


Arg Ser  Tyr Lys Leu Ile Lys  Val Pro Met Ala Ile  Ala Asn Arg 
    1160                 1165                 1170             


Val Asn  Asp Lys Gln Ile Thr  Val Thr Ala Tyr Ile  Ala Ser Leu 
    1175                 1180                 1185             


Asn Leu  Arg Asp Phe Ser Arg  Val Val Leu Glu His  Leu Pro Leu 
    1190                 1195                 1200             


Gly Thr  Leu Ile Arg Glu Ser  Asp Gly Ser Glu Phe  Tyr Ala Ala 
    1205                 1210                 1215             


Ser Ser  Glu Tyr Lys His Asn  Ala Lys Glu Leu Trp  Ala Pro Lys 
    1220                 1225                 1230             


Glu Ile  Leu Arg Ser Ser Lys  Lys Leu Asp Lys Ala  Thr Ala Asp 
    1235                 1240                 1245             


Asn Leu  Ile Asp Ile Phe Asp  Phe Leu Thr Asn Asp  Lys Thr Ile 
    1250                 1255                 1260             


Ser Asn  Phe Lys Phe Tyr Glu  Lys Asp Leu Leu His  Leu Lys Asn 
    1265                 1270                 1275             


Val Arg  Asp Lys Phe Val Glu  Ala Ser Asp Glu Ile  Lys Ile Lys 
    1280                 1285                 1290             


Val Leu  Ser Asp Val Ile Tyr  Glu Leu His Asn Asp  Ala Gly Trp 
    1295                 1300                 1305             


Arg Asp  Pro Phe Lys His Ile  Gln Glu Lys Pro Ala  Trp Thr Met 
    1310                 1315                 1320             


Leu Gln  Val Lys Gly Gly Ile  Lys Leu Ser Glu Lys  Ala Ala Ile 
    1325                 1330                 1335             


Val Tyr  Gln Ser Pro Thr Gly  Leu Phe Glu Thr Ile  Thr Ile Val 
    1340                 1345                 1350             


Gly Glu  
    1355 


<210>  934
<211>  1356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  934

Met Lys Lys Phe Glu Glu Tyr Tyr Ile Gly Leu Asp Met Gly Thr Gly 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Thr Asp Ser Ala Tyr Asn Ile Ile Lys Arg 
            20                  25                  30          


His Gly Lys Ala Leu Trp Gly Ile Arg Leu Phe Glu Ser Ala Lys Thr 
        35                  40                  45              


Ala Glu Glu Arg Arg Ile Phe Arg Thr Ser Arg Arg Arg Thr Glu Arg 
    50                  55                  60                  


Arg Lys Asn Arg Leu His Leu Leu Gln Glu Ile Phe Ala Glu Glu Ile 
65                  70                  75                  80  


Ser Lys Lys Asp Met Gly Phe Tyr Gln Arg Met Lys Glu Ser Lys Tyr 
                85                  90                  95      


Trp His Glu Asp Lys Lys Asp Ile Asn Gly Asn Met Pro Glu Leu Pro 
            100                 105                 110         


Tyr Ala Leu Phe Ala Asp Asp Gly Phe Thr Asp Lys Asp Tyr Tyr Lys 
        115                 120                 125             


Lys Tyr Pro Thr Ile Phe His Leu Arg Tyr Ala Leu Ala Thr Asn Thr 
    130                 135                 140                 


Thr Asp Lys Pro Asp Ile Arg Leu Leu Tyr Leu Ala Leu His His Ile 
145                 150                 155                 160 


Ile Lys His Arg Gly His Phe Leu Phe Glu Gly Lys Lys Leu Ser Glu 
                165                 170                 175     


Val Lys Glu Phe Ser Thr Ala Ile Glu Ala Leu Val Gln Gln Ala Glu 
            180                 185                 190         


Glu Gln Glu Ile Asp Leu Lys Ser Leu Thr Gln Glu Glu Val Leu Thr 
        195                 200                 205             


Gln Leu Glu His Ile Met Ser Asp Ala Gln Cys Thr Lys Ser Asp Lys 
    210                 215                 220                 


Lys Thr Gln Val Ser Lys Leu Ile Val Gly Thr Asp Lys Gln Lys Gln 
225                 230                 235                 240 


Lys Gln Glu Lys Ala Ile Ala Ala Leu Ile Thr Gly Cys Thr Val Lys 
                245                 250                 255     


Leu Ser Asp Ile Phe Ala Asp Pro Glu Leu Asp Lys Glu Glu Ile Ser 
            260                 265                 270         


Lys Ile Cys Phe Ser Asp Asn Gly Tyr Glu Glu Lys Glu Val Glu Ile 
        275                 280                 285             


Glu Ser Val Leu Ala Asp Arg Phe Leu Leu Ile Ala Ala Ala Lys Gly 
    290                 295                 300                 


Val Tyr Asp Trp Thr Val Leu His Asp Ile Leu Gly Asp Ala Arg Phe 
305                 310                 315                 320 


Leu Ser Glu Ala Lys Val Lys Thr Tyr Glu Lys His Arg Asp Asp Leu 
                325                 330                 335     


Arg Lys Val Lys Gln Leu Leu Arg Met Asp Lys Glu Val Tyr Glu Ala 
            340                 345                 350         


Val Phe Gly Val Ser Glu Lys Asn Glu Ala Asn Tyr Ser Ala Tyr Ile 
        355                 360                 365             


Gly Met Ala Lys Lys Asn Gly Lys Lys Gln Pro Ile Glu Lys Lys Cys 
    370                 375                 380                 


Ser Gln Glu Asp Phe Tyr Lys Phe Leu Lys Lys Gln Leu Asp Arg Leu 
385                 390                 395                 400 


Ser Val Asp Glu Met Gly Gln Glu Ile Ile Asp Glu Leu Lys Ala Glu 
                405                 410                 415     


Met Asp Leu Gly Thr Leu Phe Pro Lys Gln Met Ile Arg Asp Asn Gly 
            420                 425                 430         


Val Ile Pro Tyr Gln Leu His Glu Asn Glu Leu Asn Ala Ile Leu Asp 
        435                 440                 445             


Asn Ala Gln Lys Phe Tyr Pro Phe Leu Leu Glu Lys Asp Gln Asp Gly 
    450                 455                 460                 


Tyr Thr Pro Val Glu Lys Ile Gln Lys Ile Phe Arg Phe Arg Ile Pro 
465                 470                 475                 480 


Tyr Tyr Val Gly Pro Leu Asn Thr Ala His Gln Asp Lys Gly Gly His 
                485                 490                 495     


Ser Trp Ser Val Arg Lys Lys Glu Gly Lys Ile Tyr Pro Trp Asn Phe 
            500                 505                 510         


Ser Glu Met Ile Asp Glu Glu Glu Ser Ala Thr Lys Phe Ile Glu Arg 
        515                 520                 525             


Met Thr Asn Lys Cys Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys 
    530                 535                 540                 


Glu Ser Leu Leu Tyr Ala Lys Phe Asn Val Leu Asn Glu Leu Asn Asn 
545                 550                 555                 560 


Leu Arg Ile Asp Gly Glu Leu Ile Ala Pro Glu Leu Lys Gln Asp Ile 
                565                 570                 575     


Tyr Asn Asn Val Phe Lys Lys Tyr Gln Arg Val Thr Gly Lys Lys Val 
            580                 585                 590         


Lys Asp Tyr Leu Arg Ile Asn Gly Val Ile Ser Lys Glu Gln Glu Leu 
        595                 600                 605             


Ser Gly Phe Asp Gln Asn Phe Lys Ser Ser Leu Lys Ala Tyr His Asp 
    610                 615                 620                 


Phe Lys Gln Ile Val Gly Thr Gly Val Leu Ser Glu Ser Glu Gln Glu 
625                 630                 635                 640 


Asp Ile Val Arg Asp Ile Thr Leu Phe Ala Asp Ser Gln Lys Met Leu 
                645                 650                 655     


Lys Gln Arg Ile His Arg Lys Tyr Pro Met Leu Ser Asp Lys Gln Ile 
            660                 665                 670         


Ala Gln Leu Ala Asn Lys Lys Tyr Ala Gly Trp Gly Arg Leu Ser Arg 
        675                 680                 685             


Thr Phe Leu Glu Asn Ile Glu Ser Val Asn Lys Glu Thr Gly Glu Val 
    690                 695                 700                 


Met Asn Ile Ile Thr Ala Leu Trp Glu Thr Asn Glu Asn Leu Met Lys 
705                 710                 715                 720 


Leu Leu Ser Gly Lys Tyr Asp Tyr Thr Lys Gln Ile Glu Gln Trp Asn 
                725                 730                 735     


Ala Arg Gln Met Lys Lys Gly Glu Ile Glu Tyr Gln Asp Ile Glu Glu 
            740                 745                 750         


Leu Tyr Val Ser Pro Ala Val Lys Arg Pro Ile Trp Gln Thr Ile Lys 
        755                 760                 765             


Ile Val Arg Glu Ile Thr Asn Ile Met Gly Gly Ala Pro Lys Arg Ile 
    770                 775                 780                 


Phe Val Glu Val Ala Arg Glu Pro Gly Glu Lys Gly Val Arg Lys Val 
785                 790                 795                 800 


Ser Arg Arg Asn Gln Leu Leu Asp Leu Tyr Lys Ala Cys Lys Lys Glu 
                805                 810                 815     


Ala Pro Glu Leu Tyr Asp Arg Leu Ala Asn Lys Glu Ser Asp Asn Ala 
            820                 825                 830         


Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Ser Gln Met Gly Arg Cys 
        835                 840                 845             


Met Tyr Ser Gly Asp Ala Ile Asp Leu Asp Glu Leu Phe Thr Asn Arg 
    850                 855                 860                 


Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met Asp Asp Ser 
865                 870                 875                 880 


Ile Asp Asn Arg Val Leu Val Lys Arg Asp Leu Asn Ser Lys Lys Thr 
                885                 890                 895     


Asp Thr Phe Pro Val Ser Glu Glu Tyr Arg Lys Ala Gly Lys Ala Leu 
            900                 905                 910         


Trp Ser Thr Leu Leu Lys Lys Gly Leu Ile Ser Lys Glu Lys Tyr Lys 
        915                 920                 925             


Arg Leu Thr Arg Thr Glu Pro Phe Gly Glu Asp Glu Leu Ala Gly Phe 
    930                 935                 940                 


Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala Val Ala 
945                 950                 955                 960 


His Leu Leu Glu Glu Ala Tyr Pro Asp Ala Glu Ile Val Tyr Ala Lys 
                965                 970                 975     


Ala Arg Ala Val Ser Glu Phe Arg His Lys Phe Asp Met Ile Lys Val 
            980                 985                 990         


Arg Glu Val Asn Asp Phe His His  Ala Lys Asp Ala Tyr  Leu Asn Ile 
        995                 1000                 1005             


Val Val  Gly Asn Thr Tyr Tyr  Val Lys Phe Thr Lys  Asp Ala Ala 
    1010                 1015                 1020             


Trp Phe  Val Arg Asn Asn Pro  Gly Arg Thr Tyr Asn  Leu Lys Lys 
    1025                 1030                 1035             


Met Phe  Glu Asn Glu Thr Val  Lys Arg Gly Asn Glu  Thr Ala Trp 
    1040                 1045                 1050             


Ile Pro  Gly Asp Asn Gly Thr  Ile Cys Thr Val Lys  Lys Trp Met 
    1055                 1060                 1065             


Arg Lys  Asn Asn Val Leu Phe  Thr Arg Arg Ala Tyr  Glu Val Lys 
    1070                 1075                 1080             


Gly Gly  Leu Phe Asp Gln Asn  Ile Leu Lys Lys Gly  Lys Gly Gln 
    1085                 1090                 1095             


Ile Pro  Ile Lys Gly Gly Ala  Asp Glu Arg Leu Arg  Ser Arg Asp 
    1100                 1105                 1110             


Lys Tyr  Gly Ala Tyr Asn Lys  Ala Ser Gly Ala Tyr  Phe Met Leu 
    1115                 1120                 1125             


Val Glu  Ser Asp Gly Lys Lys  Gly Glu Arg Lys Arg  Thr Ile Glu 
    1130                 1135                 1140             


Phe Val  Pro Val Tyr Leu Ala  Lys Ser Leu Glu Glu  Ser Val Glu 
    1145                 1150                 1155             


Leu Arg  Arg Arg Tyr Cys Glu  Glu Glu Leu Gly Leu  Val Asn Pro 
    1160                 1165                 1170             


Asp Val  Arg Leu Ser Lys Ile  Arg Lys Asn Ala Leu  Ile Asp Val 
    1175                 1180                 1185             


Asp Gly  Phe Lys Met His Leu  Val Gly Arg Thr Glu  Asn Arg Leu 
    1190                 1195                 1200             


Leu Leu  Ile Gly Ala Thr Gln  Leu Val Leu Ser Glu  Asp Asp Gln 
    1205                 1210                 1215             


Gln Leu  Ile Lys Lys Ile Gly  Lys Tyr Cys Leu Asp  His Arg Glu 
    1220                 1225                 1230             


Asn Lys  Asp Ala Val Leu Ser  Glu Lys Tyr Glu Leu  Thr Met Asp 
    1235                 1240                 1245             


Leu Phe  Glu Lys Leu Tyr Asp  Ile Phe Val Phe Lys  Leu Glu Gln 
    1250                 1255                 1260             


Thr Val  Tyr Ala Val Lys Leu  Ser Lys Gln Ala Lys  Asp Leu Lys 
    1265                 1270                 1275             


Glu Val  Lys Glu Glu Phe Leu  Ala Leu Asp Asp Glu  Gln Lys Cys 
    1280                 1285                 1290             


Leu Leu  Leu Met Glu Ile Leu  His Leu Phe Gln Cys  Ser Pro Gln 
    1295                 1300                 1305             


Leu Ala  Asn Leu Thr Asn Ile  Gly Gly Gly Lys Ser  Ala Gly Ser 
    1310                 1315                 1320             


Leu Lys  Ile Leu Ser Asp Ile  Thr Lys Gln Gln Ser  Val Leu Leu 
    1325                 1330                 1335             


Ile Asn  Gln Ser Ile Thr Gly  Phe Tyr Glu Gln Val  Ile Asp Leu 
    1340                 1345                 1350             


Lys Thr  Ile 
    1355     


<210>  935
<211>  1356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  935

Met Glu Val Gly Asn Tyr Tyr Leu Gly Ile Asp Ala Gly Thr Asp Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Thr Asp Asn Gln Tyr Asn Val Ile Arg Lys Lys 
            20                  25                  30          


Gly Lys Ser Leu Trp Gly Ile Arg Leu Phe Asp Gly Ala Asn Thr Ala 
        35                  40                  45              


Gln Glu Arg Arg Thr Phe Arg Ala Leu Arg Arg Arg Asn Glu Arg Lys 
    50                  55                  60                  


Lys Gln Arg Ile Asn Leu Leu Gln Glu Leu Phe Cys Lys Glu Ile Cys 
65                  70                  75                  80  


Lys Leu Asp Pro Asn Phe Phe Ile Arg Leu Arg Asp Ser Ser Leu Trp 
                85                  90                  95      


Ser Glu Asp Lys Glu Glu Lys Gln Ile Tyr Ser Leu Phe Asn Asp Lys 
            100                 105                 110         


Asn Phe Asn Asp Arg Asn Tyr His Asn Asn Tyr Lys Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Ala Asp Leu Ile Lys Asn Lys Glu Lys His Asp Ala Arg Leu 
    130                 135                 140                 


Val Tyr Leu Ala Ile His His Ile Met Lys Asn Arg Gly His Phe Leu 
145                 150                 155                 160 


Phe Asn Gly Asn Ile Glu Ser Val Thr Ser Phe Glu Asn Thr Phe Asn 
                165                 170                 175     


Glu Phe Lys Asn Cys Leu Leu Asp Glu Leu Glu Ile Asn Leu Glu Cys 
            180                 185                 190         


Pro Ser Ile Ser Glu Phe Glu Asn Ile Leu Lys Asp Arg Thr Phe Lys 
        195                 200                 205             


Gly Thr Gln Lys Cys Lys Lys Leu Lys Glu Leu Cys Ser Ile Pro Ser 
    210                 215                 220                 


Ser Asp Lys Lys Leu Asp Asn Ile Phe Lys Leu Ile Ser Gly Leu Lys 
225                 230                 235                 240 


Ala Asp Leu Ser Ile Ile Phe Glu Asp Glu Ser Phe Lys Glu Asn Glu 
                245                 250                 255     


His Asn Lys Ile Ser Phe Ala Asp Ser Asn Tyr Glu Glu Thr Arg Glu 
            260                 265                 270         


Val Leu Asn Ser Glu Ile Gln Glu Lys Thr Leu Ile Ile Asp Ile Phe 
        275                 280                 285             


Asn Ser Ile Tyr Asn Trp Val Ile Leu Ser Asp Ile Leu Ser Gly Gly 
    290                 295                 300                 


Glu Tyr Asn Gly Gly Leu Tyr Leu Ser Ile Ala Lys Val Asn Thr Tyr 
305                 310                 315                 320 


Asn Lys His Ser Lys Asp Leu Lys Ile Leu Lys Ser Val Ile Lys Lys 
                325                 330                 335     


Tyr Phe Pro Gln Lys Tyr Thr Asp Ile Phe Ala Ser Asn Lys Glu Asn 
            340                 345                 350         


Asn Tyr Cys Ser Tyr Ile Gly Met Tyr Lys Ser Gly Gly Lys Lys Tyr 
        355                 360                 365             


Ser Val Lys Lys Cys Ser Asp Lys Pro Glu Glu Phe Tyr Lys Thr Ile 
    370                 375                 380                 


Lys Asn Ile Ile Asn Lys Asn Thr Ala Glu Glu Ile Ser Asn Asp Lys 
385                 390                 395                 400 


Asp Val Gln Tyr Ile Leu Ser Glu Ile Glu Asn Asn Ser Phe Leu Pro 
                405                 410                 415     


Leu Gln Ile Asn Lys Asn Asn Gly Val Ile Pro Tyr Gln Ala Asn Lys 
            420                 425                 430         


Ile Glu Leu Glu Ser Ile Leu Lys Asn Ala Glu Asn Tyr Leu Pro Phe 
        435                 440                 445             


Leu Lys Glu Ile Asp Thr Glu Cys Asn Lys Thr Thr Ser Glu Lys Ile 
    450                 455                 460                 


Ile Asp Leu Phe Glu Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn 
465                 470                 475                 480 


Thr Ser Lys Gly Glu Asn Ala Trp Met Ile Arg Lys Glu Asp Gly Thr 
                485                 490                 495     


Ile Arg Pro Trp Asn Phe Glu Gln Lys Val Asp Ile Asp Lys Thr Ala 
            500                 505                 510         


Ala Glu Phe Ile Thr Arg Met Thr Asn Gln Cys Thr Tyr Leu Asn Asn 
        515                 520                 525             


Glu Thr Val Leu Pro Lys Tyr Ser Leu Leu Tyr Ser Glu Tyr Thr Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Ile Arg Ile Asn Gly Glu Lys Ile Ser Val 
545                 550                 555                 560 


Glu Leu Lys Gln Asp Ile Phe Asn Ile Leu Phe Lys Asn Asn Lys Gln 
                565                 570                 575     


Val Thr Cys Lys Lys Leu Leu Ser His Leu Asn Ser Asn Gly Tyr Asn 
            580                 585                 590         


Leu Lys Lys Asp Asp Leu Ser Gly Phe Asp Gly Asn Phe Lys Ser Ser 
        595                 600                 605             


Leu Ser Ser Tyr Ile Asp Leu Lys Asn Tyr Ile Phe Gly Asp Asn Ile 
    610                 615                 620                 


Asp Lys Pro Gln Ile Tyr Asn Leu Ala Glu Lys Ile Ile Leu Trp Ile 
625                 630                 635                 640 


Thr Leu Tyr Gly Asp Asp Ser Lys Leu Leu Lys Arg Val Leu Gln Lys 
                645                 650                 655     


Asn Cys Ser Glu Gln Leu Asn Pro Glu Gln Ile Lys Lys Ile Thr Lys 
            660                 665                 670         


Leu Lys Phe Lys Gly Trp Gly Arg Leu Ser Lys Ala Phe Leu Ser Glu 
        675                 680                 685             


Leu Arg Gly Ala Asp Arg Glu Thr Gly Glu Thr Met Thr Ile Ile Lys 
    690                 695                 700                 


Gly Leu Arg Asn Thr Gln Asn Asn Leu Met Gln Leu Leu Ser Gln Asn 
705                 710                 715                 720 


Tyr Thr Phe Thr Glu Glu Cys Glu Lys Ile Asn Ser Gly Tyr Phe Ile 
                725                 730                 735     


Asn Pro Asn Glu Ile Ser Tyr Asp Ser Ile Val Lys Asn Ile Val Ala 
            740                 745                 750         


Ser Pro Ser Ile Lys Arg Ser Val Trp Gln Thr Ile Gln Ile Val Glu 
        755                 760                 765             


Glu Ile Gln Lys Val Met Gly Ser Lys Pro Arg Lys Ile Phe Val Glu 
    770                 775                 780                 


Met Ala Arg Gly Glu Glu Glu Lys Val Arg Thr Thr Ser Arg Lys Gln 
785                 790                 795                 800 


Lys Leu Leu Asp Leu Tyr Lys Asn Ile Ser Asp Glu Cys Ala Lys Gly 
                805                 810                 815     


Met Tyr Asp Glu Ile Tyr Ser Lys Asp Glu Ser Asp Phe Lys Asn Ile 
            820                 825                 830         


Arg Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Cys Cys Met Tyr Ser Gly 
        835                 840                 845             


Lys His Ile Asp Leu Ser Gln Leu Ser Asn Thr Asn Ile Trp Asp Arg 
    850                 855                 860                 


Asp His Ile Tyr Pro Gln Ser Lys Thr Lys Asp Asp Ser Leu Asp Asn 
865                 870                 875                 880 


Leu Val Leu Val Tyr Lys Ser Ile Asn Ser Glu Lys Ser Asp Asp Ile 
                885                 890                 895     


Ile Ser Pro Thr Ile Gln Asn Asn Met Arg Thr Phe Trp Lys Leu Leu 
            900                 905                 910         


Phe Asp Arg Lys Phe Ile Ser Glu Lys Lys Tyr His Arg Leu Thr Arg 
        915                 920                 925             


Thr Thr Pro Leu Thr Asp Glu Glu Leu Ala Gly Phe Ile Asn Arg Gln 
    930                 935                 940                 


Leu Val Glu Thr Arg Gln Ser Thr Lys Val Met Ala Ser Ile Phe Lys 
945                 950                 955                 960 


Arg Ile Tyr Pro Asn Thr Glu Ile Ile Tyr Val Lys Ala Lys Asn Val 
                965                 970                 975     


Ser Asp Phe Arg His Arg Lys Asp Cys Glu Asn Gly Val Lys Val Arg 
            980                 985                 990         


Glu Leu Asn Asp Tyr His His Ala  Lys Asp Ala Tyr Leu  Asn Ile Val 
        995                 1000                 1005             


Val Gly  Asn Val Tyr Ser Ser  Lys Phe Thr Asn Asn  Pro Leu Met 
    1010                 1015                 1020             


Trp Leu  Lys Asn Asn Arg Asn  Val Lys Tyr Ser Leu  Asn Arg Met 
    1025                 1030                 1035             


Tyr Asp  Phe Asn Leu Thr Val  Asn Asn Glu Asn Ile  Trp Val Val 
    1040                 1045                 1050             


Gly Asn  Glu Gly Thr Gln Lys  Thr Val Asn Lys Tyr  Leu Gly Lys 
    1055                 1060                 1065             


Asn Asp  Ile Leu Tyr Thr Arg  Tyr Ala Met Thr Asn  Lys Gly Glu 
    1070                 1075                 1080             


Leu Phe  Asp Gln Asn Pro Leu  Lys Ala Thr Gly Asp  Lys Ser Lys 
    1085                 1090                 1095             


Gln Thr  Thr Leu Val Pro Leu  Lys Lys Gly Lys Glu  Val Trp Lys 
    1100                 1105                 1110             


Tyr Gly  Gly Tyr Asn Ser Ile  Thr Pro Ser Tyr Phe  Met Leu Val 
    1115                 1120                 1125             


Glu Ser  Lys Asp Lys Lys Gly  Asn Tyr Ile Arg Thr  Ile Glu Thr 
    1130                 1135                 1140             


Met Pro  Leu Tyr Arg Glu Lys  Glu Phe Ser Lys Asn  Glu Glu Phe 
    1145                 1150                 1155             


Leu Leu  Asn Tyr Cys Lys Glu  Val His Lys Leu Lys  Glu Pro Lys 
    1160                 1165                 1170             


Ile Ile  Leu Lys Cys Ile Lys  Lys Asn Ala Leu Leu  Ile Val Asp 
    1175                 1180                 1185             


Gly Phe  Pro Met His Leu Lys  Gly Ser Thr Gly Leu  Gln Leu Lys 
    1190                 1195                 1200             


Leu Gln  Gly Ala Val Gln Leu  Ile Leu Glu Ser Asp  Met Val Ile 
    1205                 1210                 1215             


Tyr Leu  Lys Lys Leu Met Lys  Phe Val Asn Glu Asn  Asn Ile Asn 
    1220                 1225                 1230             


Lys Asn  Lys Leu Glu Val Thr  Pro Tyr Met Glu Leu  Ser Ala Lys 
    1235                 1240                 1245             


Glu Asn  Ile Lys Leu Tyr Asp  Ile Phe Ile Gln Lys  Leu Thr Thr 
    1250                 1255                 1260             


Gly Ile  Tyr Ser Lys Arg Pro  Ala Asn Pro Val Glu  Lys Leu Ile 
    1265                 1270                 1275             


Asn Ala  Arg Asp Ile Phe Ile  Glu Leu Thr Val Lys  Glu Gln Cys 
    1280                 1285                 1290             


Ile Val  Leu Ser Glu Ile Leu  His Leu Phe Gln Cys  Lys Pro Leu 
    1295                 1300                 1305             


Thr Ser  Ala Asn Leu Val Ser  Ile Asn Leu Lys Lys  Thr Val Gly 
    1310                 1315                 1320             


Ile Ile  Gln Ile Ala Lys Asn  Ile Thr Ala Lys Asn  Val Lys Leu 
    1325                 1330                 1335             


Val His  Gln Ser Pro Thr Gly  Ile Tyr Glu Tyr Thr  Val Asp Leu 
    1340                 1345                 1350             


Asn Lys  Ile 
    1355     


<210>  936
<211>  1356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  936

Met Thr Lys Lys Glu Gln Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Ser Ser Val Gly Trp Ala Val Thr Asn Asp Asn Tyr Asp Leu Leu Asn 
            20                  25                  30          


Ile Lys Lys Lys Asn Leu Trp Gly Val Arg Leu Phe Glu Glu Ala Gln 
        35                  40                  45              


Thr Ala Lys Glu Thr Arg Leu Asn Arg Ser Thr Arg Arg Arg Tyr Arg 
    50                  55                  60                  


Arg Arg Lys Asn Arg Ile Asn Trp Leu Asn Glu Ile Phe Ser Glu Glu 
65                  70                  75                  80  


Leu Ala Lys Thr Asp Pro Ser Phe Leu Ile Arg Leu Gln Asn Ser Trp 
                85                  90                  95      


Val Ser Lys Lys Asp Pro Asp Arg Lys Arg Asp Lys Tyr Asn Leu Phe 
            100                 105                 110         


Ile Asp Gly Pro Tyr Thr Asp Lys Glu Tyr Tyr Arg Glu Phe Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Lys Glu Leu Ile Leu Asn Lys Asp Lys Ala Asp 
    130                 135                 140                 


Ile Arg Leu Ile Tyr Leu Ala Leu His Asn Ile Leu Lys Tyr Arg Gly 
145                 150                 155                 160 


Asn Phe Thr Tyr Glu His Gln Lys Phe Asn Ile Ser Asn Leu Asn Asn 
                165                 170                 175     


Asn Leu Ser Lys Glu Leu Ile Glu Leu Asn Gln Gln Leu Ile Lys Tyr 
            180                 185                 190         


Asp Ile Ser Phe Pro Asp Asp Cys Asp Trp Asn His Ile Ser Asp Ile 
        195                 200                 205             


Leu Ile Gly Arg Gly Asn Ala Thr Gln Lys Ser Ser Asn Ile Leu Lys 
    210                 215                 220                 


Asp Phe Thr Leu Asp Lys Glu Thr Lys Lys Leu Leu Lys Glu Val Ile 
225                 230                 235                 240 


Asn Leu Ile Leu Gly Asn Val Ala His Leu Asn Thr Ile Phe Lys Thr 
                245                 250                 255     


Ser Leu Thr Lys Asp Glu Glu Lys Leu Asn Phe Ser Gly Lys Asp Ile 
            260                 265                 270         


Glu Ser Lys Leu Asp Asp Leu Asp Ser Ile Leu Asp Asp Asp Gln Phe 
        275                 280                 285             


Thr Val Leu Asp Ala Ala Asn Arg Ile Tyr Ser Thr Ile Thr Leu Asn 
    290                 295                 300                 


Glu Ile Leu Asn Gly Glu Ser Tyr Phe Ser Met Ala Lys Val Asn Gln 
305                 310                 315                 320 


Tyr Glu Asn His Ala Ile Asp Leu Cys Lys Leu Arg Asp Met Trp His 
                325                 330                 335     


Thr Thr Lys Asn Glu Glu Ala Val Glu Gln Ser Arg Gln Ala Tyr Asp 
            340                 345                 350         


Asp Tyr Ile Asn Lys Pro Lys Tyr Gly Thr Lys Glu Leu Tyr Thr Ser 
        355                 360                 365             


Leu Lys Lys Phe Leu Lys Val Ala Leu Pro Thr Asn Leu Ala Lys Glu 
    370                 375                 380                 


Ala Glu Glu Lys Ile Ser Lys Gly Thr Tyr Leu Val Lys Pro Arg Asn 
385                 390                 395                 400 


Ser Glu Asn Gly Val Val Pro Tyr Gln Leu Asn Lys Ile Glu Met Glu 
                405                 410                 415     


Lys Ile Ile Asp Asn Gln Ser Gln Tyr Tyr Pro Phe Leu Lys Glu Asn 
            420                 425                 430         


Lys Glu Lys Leu Leu Ser Ile Leu Ser Phe Arg Ile Pro Tyr Tyr Val 
        435                 440                 445             


Gly Pro Leu Gln Ser Ala Glu Lys Asn Pro Phe Ala Trp Met Glu Arg 
    450                 455                 460                 


Lys Ser Asn Gly His Ala Arg Pro Trp Asn Phe Asp Glu Ile Val Asp 
465                 470                 475                 480 


Arg Glu Lys Ser Ser Asn Lys Phe Ile Arg Arg Met Thr Val Thr Asp 
                485                 490                 495     


Ser Tyr Leu Val Gly Glu Pro Val Leu Pro Lys Asn Ser Leu Ile Tyr 
            500                 505                 510         


Gln Arg Tyr Glu Val Leu Asn Glu Leu Asn Asn Ile Arg Ile Thr Glu 
        515                 520                 525             


Asn Leu Lys Thr Asn Pro Ile Gly Ser Arg Leu Thr Val Glu Thr Lys 
    530                 535                 540                 


Gln Arg Ile Tyr Asn Glu Leu Phe Lys Lys Tyr Lys Lys Val Thr Val 
545                 550                 555                 560 


Lys Lys Leu Thr Lys Trp Leu Ile Ala Gln Gly Tyr Tyr Lys Asn Pro 
                565                 570                 575     


Ile Leu Ile Gly Leu Ser Gln Lys Asp Glu Phe Asn Ser Thr Leu Thr 
            580                 585                 590         


Thr Tyr Leu Asp Met Lys Lys Ile Phe Gly Ser Ser Phe Met Glu Asp 
        595                 600                 605             


Asn Lys Asn Tyr Asp Gln Ile Glu Glu Leu Ile Glu Trp Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Lys Gln Ile Leu Asn Glu Lys Leu His Ser Ser Lys Tyr 
625                 630                 635                 640 


Ser Tyr Thr Pro Asp Gln Ile Lys Lys Ile Ser Asn Met Arg Tyr Lys 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Lys Lys Ile Leu Met Asp Ile Thr Thr Glu 
            660                 665                 670         


Thr Asn Thr Pro Gln Leu Leu Gln Leu Ser Asn Tyr Ser Ile Leu Asp 
        675                 680                 685             


Leu Met Trp Ala Thr Asn Asn Asn Phe Ile Ser Ile Met Ser Asn Asp 
    690                 695                 700                 


Lys Tyr Asp Phe Lys Asn Tyr Ile Glu Asn His Asn Leu Asn Lys Asn 
705                 710                 715                 720 


Glu Asp Gln Asn Ile Ser Asp Leu Val Asn Asp Ile His Val Ser Pro 
                725                 730                 735     


Ala Leu Lys Arg Gly Ile Thr Gln Ser Ile Lys Ile Val Gln Glu Ile 
            740                 745                 750         


Val Lys Phe Met Gly His Ala Pro Lys His Ile Phe Ile Glu Val Thr 
        755                 760                 765             


Arg Glu Thr Lys Lys Ser Glu Ile Thr Thr Ser Arg Glu Lys Arg Ile 
    770                 775                 780                 


Lys Arg Leu Gln Ser Lys Leu Leu Asn Lys Ala Asn Asp Phe Lys Pro 
785                 790                 795                 800 


Gln Leu Arg Glu Tyr Leu Val Pro Asn Lys Lys Ile Gln Glu Glu Leu 
                805                 810                 815     


Lys Lys His Lys Asn Asp Leu Ser Ser Glu Arg Ile Met Leu Tyr Phe 
            820                 825                 830         


Leu Gln Asn Gly Lys Ser Leu Tyr Ser Glu Glu Ser Leu Asn Ile Asn 
        835                 840                 845             


Lys Leu Ser Asp Tyr Gln Val Asp His Ile Leu Pro Arg Thr Tyr Ile 
    850                 855                 860                 


Pro Asp Asp Ser Leu Glu Asn Lys Ala Leu Val Leu Ala Lys Glu Asn 
865                 870                 875                 880 


Gln Arg Lys Ala Asp Asp Leu Leu Leu Asn Ser Asn Val Ile Asp Arg 
                885                 890                 895     


Asn Leu Glu Arg Trp Thr Tyr Met Leu Asn Asn Asn Met Ile Gly Leu 
            900                 905                 910         


Lys Lys Phe Lys Asn Leu Thr Arg Arg Val Ile Thr Asp Lys Asp Lys 
        915                 920                 925             


Leu Gly Phe Ile His Arg Gln Leu Val Gln Thr Ser Gln Met Val Lys 
    930                 935                 940                 


Gly Val Ala Asn Ile Leu Asp Asn Met Tyr Lys Asn Gln Gly Thr Thr 
945                 950                 955                 960 


Cys Ile Gln Ala Arg Ala Asn Leu Ser Thr Ala Phe Arg Lys Ala Leu 
                965                 970                 975     


Ser Gly Gln Asp Asp Thr Tyr His Phe Lys His Pro Glu Leu Val Lys 
            980                 985                 990         


Asn Arg Asn Val Asn Asp Phe His  His Ala Gln Asp Ala  Tyr Leu Ala 
        995                 1000                 1005             


Ser Phe  Leu Gly Thr Tyr Arg  Leu Arg Arg Phe Pro  Thr Asn Glu 
    1010                 1015                 1020             


Met Leu  Leu Met Asn Gly Glu  Tyr Asn Lys Phe Tyr  Gly Gln Val 
    1025                 1030                 1035             


Lys Glu  Leu Tyr Ser Lys Lys  Lys Lys Leu Pro Asp  Ser Arg Lys 
    1040                 1045                 1050             


Asn Gly  Phe Ile Ile Ser Pro  Leu Val Asn Gly Thr  Thr Gln Tyr 
    1055                 1060                 1065             


Asp Arg  Asn Thr Gly Glu Ile  Ile Trp Asn Val Gly  Phe Arg Asp 
    1070                 1075                 1080             


Lys Ile  Leu Lys Ile Phe Asn  Tyr His Gln Cys Asn  Val Thr Arg 
    1085                 1090                 1095             


Lys Thr  Glu Ile Lys Thr Gly  Gln Phe Tyr Asp Gln  Thr Ile Tyr 
    1100                 1105                 1110             


Ser Pro  Lys Asn Pro Lys Tyr  Lys Lys Leu Ile Ala  Gln Lys Lys 
    1115                 1120                 1125             


Asp Met  Asp Pro Asn Ile Tyr  Gly Gly Phe Ser Gly  Asp Asn Lys 
    1130                 1135                 1140             


Ser Ser  Ile Thr Ile Val Lys  Ile Asp Asn Asn Lys  Ile Lys Pro 
    1145                 1150                 1155             


Val Ala  Ile Pro Ile Arg Leu  Ile Asn Asp Leu Lys  Asp Lys Lys 
    1160                 1165                 1170             


Thr Leu  Gln Asn Trp Leu Glu  Glu Asn Val Lys His  Lys Lys Ser 
    1175                 1180                 1185             


Ile Gln  Ile Ile Lys Asn Asn  Val Pro Ile Gly Gln  Ile Ile Tyr 
    1190                 1195                 1200             


Ser Lys  Lys Val Gly Leu Leu  Ser Leu Asn Ser Asp  Arg Glu Val 
    1205                 1210                 1215             


Ala Asn  Arg Gln Gln Leu Ile  Leu Pro Pro Glu His  Ser Ala Leu 
    1220                 1225                 1230             


Leu Arg  Leu Leu Gln Ile Pro  Asp Glu Asp Leu Asp  Gln Ile Leu 
    1235                 1240                 1245             


Ala Phe  Tyr Asp Lys Asn Ile  Leu Val Glu Ile Leu  Gln Glu Leu 
    1250                 1255                 1260             


Ile Thr  Lys Met Lys Lys Phe  Tyr Pro Phe Tyr Lys  Gly Glu Arg 
    1265                 1270                 1275             


Glu Phe  Leu Ile Ala Asn Ile  Glu Asn Phe Asn Gln  Ala Thr Thr 
    1280                 1285                 1290             


Ser Glu  Lys Val Asn Ser Leu  Glu Glu Leu Ile Thr  Leu Leu His 
    1295                 1300                 1305             


Ala Asn  Ser Thr Ser Ala His  Leu Ile Phe Asn Asn  Ile Glu Lys 
    1310                 1315                 1320             


Lys Ala  Phe Gly Arg Lys Thr  His Gly Leu Thr Leu  Asn Asn Thr 
    1325                 1330                 1335             


Asp Phe  Ile Tyr Gln Ser Val  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1340                 1345                 1350             


His Ile  Glu 
    1355     


<210>  937
<211>  1356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  937

Met Thr Lys Lys Glu Gln Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asn Asp Asn Tyr Asp Leu Leu Asn 
            20                  25                  30          


Ile Lys Lys Lys Asn Leu Trp Gly Val Arg Leu Phe Glu Gly Ala Gln 
        35                  40                  45              


Thr Ala Lys Glu Thr Arg Leu Asn Arg Ser Thr Arg Arg Arg Tyr Arg 
    50                  55                  60                  


Arg Arg Lys Asn Arg Ile Asn Trp Leu Asn Glu Ile Phe Ser Glu Glu 
65                  70                  75                  80  


Leu Ala Asn Thr Asp Pro Ser Phe Leu Ile Arg Leu Gln Asn Ser Trp 
                85                  90                  95      


Val Ser Lys Lys Asp Pro Asp Arg Lys Arg Asp Lys Tyr Asn Leu Phe 
            100                 105                 110         


Ile Asp Asn Pro Tyr Thr Asp Lys Glu Tyr Tyr Arg Glu Phe Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Lys Glu Leu Ile Ile Asn Lys Asn Lys Ala Asp 
    130                 135                 140                 


Ile Arg Leu Val Tyr Leu Ala Leu His Asn Ile Leu Lys Tyr Arg Gly 
145                 150                 155                 160 


Asn Phe Thr Tyr Glu His Gln Lys Phe Asn Ile Ser Thr Leu Asn Ser 
                165                 170                 175     


Asn Leu Ser Lys Glu Leu Ile Glu Leu Asn Gln Gln Leu Ile Lys Tyr 
            180                 185                 190         


Asp Ile Ser Phe Pro Asp Asn Cys Asp Trp Asn His Ile Ser Asp Ile 
        195                 200                 205             


Leu Ile Gly Arg Gly Asn Ala Thr Gln Lys Ser Ser Asn Ile Leu Asn 
    210                 215                 220                 


Asn Phe Thr Leu Asp Lys Glu Thr Lys Lys Leu Leu Lys Glu Val Ile 
225                 230                 235                 240 


Asn Leu Ile Leu Gly Asn Val Ala His Leu Asn Thr Ile Phe Lys Thr 
                245                 250                 255     


Ser Leu Thr Lys Asp Glu Glu Lys Leu Ser Phe Ser Gly Lys Asp Ile 
            260                 265                 270         


Glu Ser Lys Leu Asp Asp Leu Asp Ser Ile Leu Asp Asp Asp Gln Phe 
        275                 280                 285             


Thr Val Leu Asp Thr Ala Asn Arg Ile Tyr Ser Thr Ile Thr Leu Asn 
    290                 295                 300                 


Glu Ile Leu Asn Gly Glu Ser Tyr Phe Ser Met Ala Lys Val Asn Gln 
305                 310                 315                 320 


Tyr Glu Asn His Ala Ile Asp Leu Cys Lys Leu Arg Asp Met Trp His 
                325                 330                 335     


Thr Thr Lys Asn Glu Lys Ala Val Gly Leu Ser Arg Gln Ala Tyr Asp 
            340                 345                 350         


Asp Tyr Ile Asn Lys Pro Lys Tyr Gly Thr Lys Glu Leu Tyr Thr Ser 
        355                 360                 365             


Leu Lys Lys Phe Leu Lys Val Ala Leu Pro Thr Asn Leu Ala Lys Glu 
    370                 375                 380                 


Ala Glu Glu Lys Ile Ser Lys Gly Thr Tyr Leu Val Lys Pro Arg Asn 
385                 390                 395                 400 


Ser Glu Asn Gly Val Val Pro Tyr Gln Leu Asn Lys Ile Glu Met Glu 
                405                 410                 415     


Lys Ile Ile Asp Asn Gln Ser Gln Tyr Tyr Pro Phe Leu Lys Glu Asn 
            420                 425                 430         


Lys Glu Lys Leu Leu Ser Ile Leu Ser Phe Arg Ile Pro Tyr Tyr Val 
        435                 440                 445             


Gly Pro Leu Gln Ser Ser Glu Lys Asn Pro Phe Ala Trp Met Glu Arg 
    450                 455                 460                 


Lys Ser Asn Gly His Ala Arg Pro Trp Asn Phe Asp Glu Ile Val Asp 
465                 470                 475                 480 


Arg Glu Lys Ser Ser Asn Lys Phe Ile Arg Arg Met Thr Val Thr Asp 
                485                 490                 495     


Ser Tyr Leu Val Gly Glu Pro Val Leu Pro Lys Asn Ser Leu Ile Tyr 
            500                 505                 510         


Gln Arg Tyr Glu Val Leu Asn Glu Leu Asn Asn Ile Arg Ile Thr Glu 
        515                 520                 525             


Asn Leu Lys Thr Asn Pro Thr Gly Ser Arg Leu Thr Val Glu Thr Lys 
    530                 535                 540                 


Gln His Ile Tyr Asn Glu Leu Phe Lys Asn Tyr Lys Lys Ile Thr Val 
545                 550                 555                 560 


Lys Lys Leu Thr Lys Trp Leu Ile Ala Gln Gly Tyr Tyr Lys Asn Pro 
                565                 570                 575     


Ile Leu Ile Gly Leu Ser Gln Lys Asp Glu Phe Asn Ser Thr Leu Thr 
            580                 585                 590         


Thr Tyr Leu Asp Met Lys Lys Ile Phe Gly Ser Ser Phe Met Glu Asn 
        595                 600                 605             


Asn Lys Asn Tyr Asn Gln Ile Glu Glu Leu Ile Glu Trp Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Lys Gln Ile Leu Asn Glu Lys Leu His Ser Ser Asn Tyr 
625                 630                 635                 640 


Ser Tyr Thr Ser Asp Gln Ile Lys Lys Ile Ser Asn Met Arg Tyr Lys 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Lys Lys Ile Leu Thr Cys Ile Thr Thr Glu 
            660                 665                 670         


Thr Asn Thr Pro Lys Ser Leu Gln Leu Ser Asn Tyr Ser Val Leu Asp 
        675                 680                 685             


Leu Met Trp Thr Thr Asn Asn Asn Phe Ile Ser Ile Ile Ser Asn Asp 
    690                 695                 700                 


Lys Tyr Asp Phe Lys Asn Tyr Ile Glu Asn His Asn Leu Asn Lys Asn 
705                 710                 715                 720 


Glu Asp Gln Asn Ile Ser Asn Leu Val Asn Asp Ile His Val Ser Pro 
                725                 730                 735     


Ala Leu Lys Arg Gly Ile Thr Gln Ser Ile Lys Ile Val Gln Glu Ile 
            740                 745                 750         


Val Lys Phe Met Gly His Ala Pro Lys Tyr Ile Phe Ile Glu Val Thr 
        755                 760                 765             


Arg Glu Thr Lys Lys Ser Glu Ile Thr Thr Ser Arg Gly Lys Arg Ile 
    770                 775                 780                 


Gln Arg Leu Gln Ser Lys Leu Leu Asn Lys Ala Asn Gly Phe Lys Pro 
785                 790                 795                 800 


Gln Leu Arg Lys Tyr Leu Val Pro Asn Glu Lys Ile Gln Glu Glu Leu 
                805                 810                 815     


Lys Lys His Lys Asn Asp Leu Ser Ser Glu Arg Ile Met Leu Tyr Phe 
            820                 825                 830         


Leu Gln Asn Gly Lys Ser Leu Tyr Ser Glu Glu Ser Leu Asn Ile Asn 
        835                 840                 845             


Lys Leu Ser Asp Tyr Gln Val Asp His Ile Leu Pro Arg Thr Tyr Ile 
    850                 855                 860                 


Pro Asp Asp Ser Leu Glu Asn Lys Ala Leu Val Leu Ala Lys Glu Asn 
865                 870                 875                 880 


Gln Arg Lys Ala Asp Asp Leu Leu Leu Asn Ser Asn Val Ile Asp Lys 
                885                 890                 895     


Asn Leu Glu Arg Trp Thr Tyr Met Leu Asn Asn Asn Met Met Gly Leu 
            900                 905                 910         


Lys Lys Phe Lys Asn Leu Thr Arg Arg Val Ile Thr Asp Lys Asp Lys 
        915                 920                 925             


Leu Gly Phe Ile His Arg Gln Leu Val Gln Thr Ser Gln Met Val Lys 
    930                 935                 940                 


Gly Val Ala Asn Ile Leu Asn Ser Met Tyr Lys Asn Gln Gly Thr Thr 
945                 950                 955                 960 


Cys Ile Gln Ala Arg Ala Asn Leu Ser Thr Ala Phe Arg Lys Ala Leu 
                965                 970                 975     


Ser Asp Gln Asp Asp Arg Tyr His Phe Lys His Pro Glu Leu Val Lys 
            980                 985                 990         


Asn Arg Asn Ile Asn Asp Phe His  His Ala Gln Asp Ala  Tyr Leu Ala 
        995                 1000                 1005             


Ser Phe  Leu Gly Thr Tyr Arg  Leu Arg Arg Phe Pro  Thr Asp Glu 
    1010                 1015                 1020             


Met Leu  Leu Met Asn Gly Glu  Tyr Asn Lys Phe Tyr  Gly Gln Val 
    1025                 1030                 1035             


Lys Glu  Leu Tyr Ser Lys Lys  Lys Lys Leu Pro Asp  Ser Arg Lys 
    1040                 1045                 1050             


Asn Gly  Phe Ile Ile Ser Pro  Leu Val Asn Gly Thr  Thr Gln Tyr 
    1055                 1060                 1065             


Asp Arg  Asn Thr Gly Glu Ile  Ile Trp Asn Val Gly  Phe Arg Asp 
    1070                 1075                 1080             


Lys Ile  Leu Lys Ile Phe Asn  Tyr His Gln Cys Asn  Val Thr Arg 
    1085                 1090                 1095             


Lys Thr  Glu Ile Lys Thr Gly  Gln Phe Tyr Asp Gln  Thr Ile Tyr 
    1100                 1105                 1110             


Ser Pro  Lys Asn Pro Lys Tyr  Lys Lys Leu Ile Ala  Gln Lys Lys 
    1115                 1120                 1125             


Asp Met  Asp Pro Asn Ile Tyr  Gly Gly Phe Ser Gly  Asp Asn Lys 
    1130                 1135                 1140             


Ser Ser  Ile Thr Ile Val Lys  Ile Asp Asn Asn Lys  Ile Lys Pro 
    1145                 1150                 1155             


Val Ala  Ile Pro Ile Arg Leu  Ile Asn Asp Leu Lys  Asp Lys Lys 
    1160                 1165                 1170             


Thr Leu  Gln Asn Trp Leu Glu  Glu Asn Val Lys His  Lys Lys Ser 
    1175                 1180                 1185             


Ile Gln  Ile Ile Lys Asn Asn  Val Pro Ile Gly Gln  Ile Ile Tyr 
    1190                 1195                 1200             


Ser Lys  Lys Val Gly Leu Leu  Ser Leu Asn Ser Asp  Arg Glu Ile 
    1205                 1210                 1215             


Ala Asn  Arg Gln Gln Leu Ile  Leu Pro Pro Glu His  Ser Ala Leu 
    1220                 1225                 1230             


Leu Arg  Ile Leu Gln Ile Pro  Asp Glu Asp Pro Asp  Gln Ile Leu 
    1235                 1240                 1245             


Ala Phe  Tyr Asp Lys Asn Ile  Leu Val Glu Ile Leu  Gln Glu Leu 
    1250                 1255                 1260             


Ile Thr  Lys Met Lys Lys Phe  Tyr Pro Phe Tyr Lys  Asn Glu Gln 
    1265                 1270                 1275             


Glu Phe  Leu Ala Ser Asn Ile  Glu Asn Phe Asn Gln  Ala Thr Thr 
    1280                 1285                 1290             


Ser Glu  Lys Ile Asn Ser Leu  Glu Glu Leu Ile Thr  Leu Leu His 
    1295                 1300                 1305             


Ala Asn  Ser Thr Ser Ala His  Leu Ile Phe Asn Asn  Ile Glu Lys 
    1310                 1315                 1320             


Lys Ala  Phe Gly Arg Lys Thr  His Gly Leu Thr Leu  Asn Asp Thr 
    1325                 1330                 1335             


Asp Phe  Ile Tyr Gln Ser Val  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1340                 1345                 1350             


His Ile  Glu 
    1355     


<210>  938
<211>  1356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  938

Met Ser Lys Lys Asp Ser Glu Tyr Tyr Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asn Ser Val Gly Trp Ala Val Thr Asn Gly Lys Tyr Asp Leu Leu Asn 
            20                  25                  30          


Ile Lys Lys Lys Asn Leu Trp Gly Val Arg Leu Phe Glu Ala Ala Glu 
        35                  40                  45              


Ser Ala Ala Gly Arg Arg Ile Asn Arg Ser Thr Arg Arg Arg Tyr Arg 
    50                  55                  60                  


Arg Arg Arg Asn Arg Ile Asn Trp Leu Asn Glu Ile Phe Ala Asp Glu 
65                  70                  75                  80  


Leu Ser Lys Val Asp Pro Ser Phe Leu Thr Arg Leu Asn Ser Ser Trp 
                85                  90                  95      


Ile Ser Lys Lys Asp Ser Ser Arg Thr Arg Asp Lys Tyr Asn Leu Phe 
            100                 105                 110         


Met Asp Ala Asp Tyr Thr Asp Lys Asn Tyr His His Lys Tyr Pro Thr 
        115                 120                 125             


Ile Tyr His Leu Arg Arg Asp Leu Met Phe Asp Asp Lys Gln Phe Asp 
    130                 135                 140                 


Ile Arg Leu Val Tyr Leu Ala Leu His Asn Ile Ile Lys Tyr Arg Gly 
145                 150                 155                 160 


Asn Phe Thr Tyr Asp His Arg Lys Phe Asn Val Ser Glu Met Ser Ser 
                165                 170                 175     


Asn Leu Thr Lys Ala Leu Glu Asn Phe Asn Gln Glu Leu Met Glu Phe 
            180                 185                 190         


Glu Asp Ala Phe Pro Asp Asn Thr Asp Tyr Ser Ala Ile Ser Glu Val 
        195                 200                 205             


Leu Leu Lys Ser Asn Asn Val Ser Thr Lys Ile Asp Glu Ile Ile Ser 
    210                 215                 220                 


Asn Leu Glu Leu Asp Lys Asp Thr Lys Lys Tyr Leu Lys Glu Val Leu 
225                 230                 235                 240 


Lys Leu Ile Leu Gly Asn Ser Ala Asn Leu Asn Thr Ile Phe His Thr 
                245                 250                 255     


Ser Leu Asp Lys Asp Asp Glu Lys Leu Ser Leu Ser Ala Lys Asp Ile 
            260                 265                 270         


Glu Leu Lys Leu Asn Asn Leu Asp Ser Leu Leu Ser Asp Glu Gln Ile 
        275                 280                 285             


His Phe Ile Ser Ser Ala Asn Glu Leu Tyr Ser Ser Ile Thr Leu Asn 
    290                 295                 300                 


Asp Ile Leu Asp Gly Lys Thr Tyr Leu Ser Leu Ala Lys Ile Lys Gln 
305                 310                 315                 320 


Tyr Asp Thr His Cys Ser Asp Leu Gln Glu Leu Lys Glu Met Trp Tyr 
                325                 330                 335     


Lys Thr Thr Asn Pro Asp Leu Ile Lys Lys Ser Arg Lys Ala Tyr Lys 
            340                 345                 350         


Glu Tyr Val His Gly Ser Tyr Asn Thr Glu Asp Phe Tyr Lys Ala Ile 
        355                 360                 365             


Ser Pro Phe Leu Lys Ile Ala Ile Pro Glu Asp Leu Ala Lys Asp Ala 
    370                 375                 380                 


Leu Lys Lys Ile Asp Ala Asn Thr Tyr Leu Pro Lys Gln Arg Thr Ser 
385                 390                 395                 400 


Asn Asn Gly Val Ile Pro Phe Gln Leu Asn Leu Ile Glu Leu Glu Lys 
                405                 410                 415     


Ile Ile Asp Asn Gln Ser Lys Tyr Tyr Pro Phe Leu Lys Glu Asn Arg 
            420                 425                 430         


Glu Lys Ile Ile Ser Ile Leu Ser Phe Arg Ile Pro Tyr Tyr Val Gly 
        435                 440                 445             


Pro Ile Gln Ala Pro Thr Asp Lys Asn Phe Ser Trp Met Val Lys Lys 
    450                 455                 460                 


Ile Gln Ala Lys Ala Arg Pro Trp Asn Phe Asp Glu Val Ile Asp Arg 
465                 470                 475                 480 


Glu Lys Ser Ser Asn Asn Phe Ile Lys Arg Met Thr Thr Thr Asp Thr 
                485                 490                 495     


Tyr Leu Val Gly Glu Pro Val Leu Pro Lys Asn Ser Leu Ile Tyr Gln 
            500                 505                 510         


Lys Phe Glu Val Leu Asn Glu Leu Asn Asn Ile Arg Ile Ser Lys Asn 
        515                 520                 525             


Leu Ser Gln Asp Pro Leu Gly Ser Lys Leu Ser Val Glu Val Lys Gln 
    530                 535                 540                 


Gln Ile Phe Glu Glu Leu Phe Lys Arg Tyr Lys Thr Val Lys Ser Asp 
545                 550                 555                 560 


Gln Leu Lys Lys Trp Leu Ile Arg Asn Ser Tyr Phe Thr Asp Pro Thr 
                565                 570                 575     


Ile Ile Gly Leu Ser Lys Pro Ser Glu Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         


Phe Ile Asp Met Lys Ala Ile Phe Gly Met Asp Phe Val Asn Asn Pro 
        595                 600                 605             


Arg Asn Ser Ser Gln Leu Glu Glu Leu Ala Glu Trp Leu Thr Val Phe 
    610                 615                 620                 


Glu Asp Lys Gln Ile Leu Ser Glu Lys Ile Arg His Ser Asn Tyr Pro 
625                 630                 635                 640 


Tyr Ser Asp Gln Gln Ile Arg Lys Ile Ser Asn Met Arg Tyr Arg Gly 
                645                 650                 655     


Trp Gly Arg Ile Ser Lys Lys Leu Leu Cys Asp Ile Lys Val Glu Ser 
            660                 665                 670         


Thr Ile Tyr Lys Ser His Gln Leu His Pro Tyr Ser Ile Leu Asp Leu 
        675                 680                 685             


Met Trp Ser Thr Asn Asn Asn Phe Tyr Ser Ile Leu His Lys Asp Lys 
    690                 695                 700                 


Tyr Asn Phe Glu Ser Ile Leu Glu Asp Tyr Asn Leu Gly Thr Asp Asp 
705                 710                 715                 720 


Lys Thr Asp Ile Asn Glu Leu Val Asp Asn Ile His Thr Ser Pro Ala 
                725                 730                 735     


Leu Lys Arg Gly Ile Ile Gln Ser Ile Asn Ile Val Lys Glu Leu Val 
            740                 745                 750         


Lys Phe Met Gly His Ala Pro Gln His Ile Phe Leu Glu Phe Thr Arg 
        755                 760                 765             


Glu Asp Lys Thr Ser Thr Leu Ser Glu Ser Arg Lys Lys Arg Leu Lys 
    770                 775                 780                 


Lys Leu Tyr Asn Asn Leu Ser Glu Asp Thr Lys Lys Phe Lys Leu Asp 
785                 790                 795                 800 


Leu Arg Asp Tyr Leu Val Pro Asp Lys Ser Ile Gln Lys Glu Leu Glu 
                805                 810                 815     


Ser His Asp Lys Asp Leu Ser Lys Glu Arg Leu Met Leu Tyr Phe Leu 
            820                 825                 830         


Gln Asn Gly Lys Ser Leu Tyr Ser Asp Glu Ala Leu Asp Ile Asn Arg 
        835                 840                 845             


Leu Ser Glu Tyr Gln Val Asp His Ile Leu Pro Arg Thr Tyr Ile Lys 
    850                 855                 860                 


Asp Asp Ser Leu Glu Asn Lys Ala Leu Val Leu Ala Lys Glu Asn Gln 
865                 870                 875                 880 


Arg Lys Ala Asp Asp Leu Leu Leu Gln Pro Ser Ile Ile Asn Lys Asn 
                885                 890                 895     


Met Glu Arg Trp Ile Tyr Met Lys Asp His Gly Met Ile Ser Pro Lys 
            900                 905                 910         


Lys Phe Lys Asn Leu Thr Arg Thr Thr Ile Thr Glu Asp Asp Lys Val 
        915                 920                 925             


His Phe Ile Asn Arg Gln Leu Val Gln Thr Ser Gln Ile Ile Lys Asn 
    930                 935                 940                 


Val Ala Asn Ile Leu Asp Asn Met Tyr Lys Glu Gln Gly Thr Thr Cys 
945                 950                 955                 960 


Val Glu Thr Lys Ala Asn Leu Ser Ser Ala Phe Arg Thr Ala Phe Ser 
                965                 970                 975     


Glu Gln Asp Asp Lys Tyr His Phe Lys His Pro Glu Phe Ile Lys Asn 
            980                 985                 990         


Arg Asp Val Asn Asp Phe His His  Ala Gln Asp Ala Tyr  Leu Ala Cys 
        995                 1000                 1005             


Leu Leu  Gly Leu Tyr Arg Ile  Gln Arg Phe Pro Ser  Asp Glu Met 
    1010                 1015                 1020             


Leu Leu  Ile Lys Lys Glu Tyr  Arg Lys Phe Phe Ala  Gln Ala Gln 
    1025                 1030                 1035             


Asn Ser  Val Lys Asn His Gly  Lys Ile Pro Asp Ser  Gln Lys Asn 
    1040                 1045                 1050             


Gly Phe  Ile Leu Ala Pro Leu  Val Asn Gly Glu Thr  Leu Ile Asn 
    1055                 1060                 1065             


Asn Lys  Thr Met Glu Thr Ile  Trp Asn Met Asp Tyr  Lys Arg Lys 
    1070                 1075                 1080             


Val Ile  Lys Ile Phe Asn Tyr  Lys Gln Tyr Asn Ile  Thr Lys Arg 
    1085                 1090                 1095             


Thr Glu  Ile Lys Thr Gly Glu  Phe Tyr Lys Gln Thr  Leu Tyr Ser 
    1100                 1105                 1110             


Pro His  Asp Glu Asn Val Lys  Asn Pro Ile Pro Gln  Lys Lys Asn 
    1115                 1120                 1125             


Leu Asp  Pro Lys Ile Tyr Gly  Gly Tyr Ser Gly Val  Gln Ser Ser 
    1130                 1135                 1140             


Tyr Leu  Ala Ile Ile Lys Val  Asp Gln Lys Lys Pro  Thr Met Val 
    1145                 1150                 1155             


Ser Val  Pro Ile Arg Phe Ser  Lys Met Ile Asp Asp  Gly Lys Leu 
    1160                 1165                 1170             


Ser Leu  Gln Ser Trp Leu Glu  Asn Asn Ile Lys His  Lys Lys Thr 
    1175                 1180                 1185             


Ile Glu  Val Leu Lys Thr Lys  Ile Pro Ile Gly Gln  Leu Thr Tyr 
    1190                 1195                 1200             


Asn Pro  Lys Asn Gly Tyr Leu  Ser Val Gln Ser Glu  Lys Glu Ile 
    1205                 1210                 1215             


Ala Asn  Ala Gln Gln Leu Phe  Leu Pro Phe Glu His  Val Ala Leu 
    1220                 1225                 1230             


Leu Thr  Leu Leu His Ala Pro  Thr Ser Glu Tyr Asp  Gln Ile Leu 
    1235                 1240                 1245             


Ser Phe  Tyr Asp Thr Asp Ile  Leu Leu Glu Ile Phe  Asp Glu Ile 
    1250                 1255                 1260             


Thr Asn  Lys Ile Lys Thr Phe  Phe Pro Phe Tyr Ile  Asp Glu Gln 
    1265                 1270                 1275             


Glu Tyr  Leu Val Ser Ile Arg  Glu Asn Phe Gln Ser  Ala Thr Val 
    1280                 1285                 1290             


Ile Glu  Lys Val Asn Val Leu  Gln Gln Leu Leu Ile  Met Leu His 
    1295                 1300                 1305             


Ala Asn  Ser Ser Arg Ile Ser  Ile Lys Leu Gly Glu  Val Ser Lys 
    1310                 1315                 1320             


Lys Asp  Leu Gly Arg Lys Lys  Lys Arg Ile Pro Leu  Glu Asn Thr 
    1325                 1330                 1335             


Asp Leu  Val Tyr Gln Ser Val  Thr Gly Leu Tyr Gln  Thr Lys Phe 
    1340                 1345                 1350             


His Ile  Asp 
    1355     


<210>  939
<211>  1357
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  939

Met Asn Asp Arg Asn Lys Lys Tyr Asn Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asp Asn Asn Tyr Asn Leu Leu His 
            20                  25                  30          


Ala Lys Lys Lys Asn Leu Trp Gly Ile Arg Leu Phe Asp Gly Ala Glu 
        35                  40                  45              


Thr Ala Ala Ser Arg Arg Thr Phe Arg Ser Thr Arg Arg Arg Tyr Arg 
    50                  55                  60                  


Arg Arg Arg Asn Arg Ile Asn Trp Leu Asn Glu Ile Phe Ala Asp Glu 
65                  70                  75                  80  


Leu Ala Lys Lys Asp Gln Ser Phe Leu Val Arg Met Asn Ser Ser Trp 
                85                  90                  95      


Val Ser Lys Lys Asp Lys Ile Arg Gln Arg Asp Gln Tyr Asn Leu Phe 
            100                 105                 110         


Met Asp Ser Asp Tyr Thr Asp Val Glu Tyr His Arg Glu Phe Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Lys Arg Leu Ile Glu Asp Lys Ser Gln Ala Asp 
    130                 135                 140                 


Ile Arg Leu Val Tyr Leu Ala Leu His Asn Ile Ile Lys Tyr Arg Gly 
145                 150                 155                 160 


Asn Phe Thr Tyr Glu His Gln Asn Phe Asp Val Ser His Met Asp Ser 
                165                 170                 175     


Gly Leu Ala Leu Leu Leu Asn Gln Leu Asn Gln Gln Leu Gln Ala Phe 
            180                 185                 190         


Glu Val Ala Phe Pro Asp Glu Thr Asn Phe Glu Ser Met Ser Thr Ile 
        195                 200                 205             


Leu Leu Glu Lys Lys Thr Pro Thr Gln Lys Ile Asp Glu Ile Val Asp 
    210                 215                 220                 


Gln Ile Gln Pro Glu Lys Asp Leu Leu Gln Ile Thr Lys Asn Ile Met 
225                 230                 235                 240 


Lys Met Leu Leu Gly Asn Lys Ala Asp Leu Val Lys Ile Phe Ser Leu 
                245                 250                 255     


Asp Ser Asp Asp Lys Ile Thr Val Glu Phe Ser Ser Asn Ser Ile Glu 
            260                 265                 270         


Gln Asp Leu Ser Asn Leu Glu Glu Thr Leu Ser Asp Glu Gln Phe Asn 
        275                 280                 285             


Ile Ile Thr Leu Ala Asn Ser Ile Tyr Ser Ser Ile Val Leu Asn Asp 
    290                 295                 300                 


Ile Leu Ser Gly Glu Thr Tyr Leu Ser Phe Ala Lys Ala Arg Gln Tyr 
305                 310                 315                 320 


Ser Asp His Lys Lys Asp Leu Ala Lys Leu Lys Thr Met Trp Arg Glu 
                325                 330                 335     


Ser Pro Asp Arg Lys Ala Val Gly Lys Ala Lys Lys Ala Tyr Asp Asp 
            340                 345                 350         


Tyr Leu His Ser Gly Lys Tyr Gly Ile Glu Asp Phe Tyr Lys Asp Met 
        355                 360                 365             


Ser Lys Phe Leu Glu Thr Ala Thr Pro Val Glu Leu Ala Lys Glu Ala 
    370                 375                 380                 


Asn Glu Lys Ile Leu Asn Lys Ser Tyr Leu Leu Lys Gln Arg Asn Asn 
385                 390                 395                 400 


Glu Asn Gly Val Ile Pro Phe Gln Leu Asn Lys Asn Glu Met Ile Glu 
                405                 410                 415     


Ile Ile Asn Asn Gln Ala Lys Tyr Tyr Pro Phe Leu Ala Glu Asn Lys 
            420                 425                 430         


Asp Lys Leu Leu Ser Ile Leu Ser Phe Arg Ile Pro Tyr Tyr Val Gly 
        435                 440                 445             


Pro Leu Gln Ser Lys Lys Asp Asn Pro Phe Ala Trp Met Glu Lys Lys 
    450                 455                 460                 


Ser Gln Gly Lys Ile Arg Pro Trp Asn Phe Asp Glu Ile Val Asp Arg 
465                 470                 475                 480 


Glu Ala Ser Ser Asn Asn Phe Ile Arg Arg Met Thr Ser Thr Asp Thr 
                485                 490                 495     


Tyr Leu Ile Gly Glu Pro Val Leu Pro Leu Lys Ser Pro Ile Tyr Gln 
            500                 505                 510         


Lys Tyr Glu Val Leu Asn Glu Leu Ser Asn Val Arg Ile Asn Ala Asp 
        515                 520                 525             


Glu Val Asp Asn Pro Leu Gly Asp Lys Leu Ser Val Asp Leu Lys Gln 
    530                 535                 540                 


Ala Ile Tyr Asn Glu Leu Phe Leu Lys His Arg Ser Val Ser Lys Ala 
545                 550                 555                 560 


Leu Leu Thr Lys Trp Leu Ile Asn Gln Ser Tyr Phe Gln Asn Pro Gln 
                565                 570                 575     


Ile Ser Gly Leu Ala Asp Ser Lys Lys Phe Asn Ser Gly Leu Thr Thr 
            580                 585                 590         


Tyr His Asp Phe Lys Lys Ile Phe Gly Glu Asp Phe Val Asn Asp Pro 
        595                 600                 605             


Thr Asn Gln Ser Gln Leu Glu Gln Leu Ala Glu Trp Leu Thr Ile Phe 
    610                 615                 620                 


Glu Asp Lys Lys Ile Leu Lys Ile Lys Leu Asp Asp Ser Gln Phe Asn 
625                 630                 635                 640 


Cys Thr Pro Glu Gln Ile Lys Lys Ile Ala Gly Met Arg Tyr Gln Gly 
                645                 650                 655     


Trp Gly Arg Leu Ser Lys Lys Leu Leu Cys Asp Ile Lys Thr Thr Thr 
            660                 665                 670         


Val Thr Ser Lys Asn His Gln Gln Ala Ser Tyr Ser Ile Leu Asp Leu 
        675                 680                 685             


Met Trp Asn Thr Asn Arg Asn Phe Ile Ser Val Leu Lys Asn Asp Thr 
    690                 695                 700                 


Tyr Lys Phe Glu Asp Lys Ile Gln Thr Phe Asn Leu Asp Lys Asn Glu 
705                 710                 715                 720 


Ala Ala Thr Pro Gln Ala Met Ile Asp Glu Leu Gln Thr Ser Pro Ala 
                725                 730                 735     


Leu Lys Arg Gly Ile Arg Gln Ser Val Thr Ile Ile Gln Glu Leu Val 
            740                 745                 750         


Lys Phe Met Gly His Ala Pro Glu His Ile Phe Leu Glu Phe Thr Arg 
        755                 760                 765             


Glu Asp Asp Val Ser Ser Leu Thr Lys Ser Arg Asp Ser Arg Leu Asn 
    770                 775                 780                 


Lys Leu Tyr Ala Arg Ile Ala Lys Asn Thr Lys Lys Thr Asp Asp Glu 
785                 790                 795                 800 


Leu Lys Lys Ser Leu Leu Pro Ser Ser Asp Ile Lys Ala Thr Leu Lys 
                805                 810                 815     


Ala Asn Lys Asn Asn Leu Ser Asn Asp Arg Leu Met Leu Tyr Tyr Leu 
            820                 825                 830         


Gln Met Gly Lys Ser Leu Tyr Ser Asn Gln Pro Leu Asp Ile Asn Arg 
        835                 840                 845             


Leu Ser Glu Tyr Gln Ile Asp His Ile Leu Pro Gln Ser Tyr Ile Lys 
    850                 855                 860                 


Asp Asn Ser Leu Glu Asn Arg Ala Leu Val Leu Ala Ser Glu Asn Gln 
865                 870                 875                 880 


His Lys Ser Ser Asp Leu Leu Leu Lys Pro Glu Ile Ile Asn Gln Asn 
                885                 890                 895     


Ile Ala Arg Trp Arg Tyr Met Lys Asp Ala Gly Leu Met Gly Pro Lys 
            900                 905                 910         


Lys Phe Lys Asn Leu Thr Arg Ile Gln Ile Thr Asp Ser Asp Gln Asn 
        915                 920                 925             


Asn Phe Ile Asn Arg Gln Leu Val Gln Thr Ser Gln Ile Ile Lys His 
    930                 935                 940                 


Val Ser Asn Ile Leu Asp Ser Met Tyr Gly Asp Gln Gly Thr Gln Cys 
945                 950                 955                 960 


Val Glu Thr Arg Ala His Leu Ser Thr Glu Phe Arg Lys Ala Phe Ser 
                965                 970                 975     


Asn Glu Asp Asp Asn Tyr His Phe Gln His Pro Glu Phe Val Lys Asn 
            980                 985                 990         


Arg Asn Ile Asn Asp Tyr His His  Ala Gln Asp Ala Tyr  Leu Ala Cys 
        995                 1000                 1005             


Leu Leu  Gly Leu Tyr Gln Met  Lys Lys Tyr Pro Thr  Asp Asn Met 
    1010                 1015                 1020             


Ile Leu  Leu Lys Lys Glu Tyr  Lys Arg Phe Phe Ser  Asn Thr Lys 
    1025                 1030                 1035             


Lys Ile  Tyr Asp Lys Lys His  His Phe Pro Asp Tyr  Val Lys Asn 
    1040                 1045                 1050             


Gly Trp  Ile Ile Gly Ser Met  Phe Asn Gly Glu Lys  Tyr Val Asp 
    1055                 1060                 1065             


Glu Glu  Thr Gly Gln Ile Ile  Trp Asp Gln Gln Thr  Lys Asp Met 
    1070                 1075                 1080             


Val Ser  Cys Ile Phe Gln Tyr  Lys Gln Tyr Asn Val  Thr Lys Arg 
    1085                 1090                 1095             


Thr Glu  Ala Ala Asn Gly Glu  Phe Tyr Asn Gln Thr  Val Tyr Lys 
    1100                 1105                 1110             


Lys Asp  Pro Asn Glu Lys Arg  Lys Leu Ile Ser Leu  Lys Gln Gly 
    1115                 1120                 1125             


Leu Asp  Thr Lys Leu Tyr Gly  Gly Tyr Ser Gly Asp  Asn Pro Ala 
    1130                 1135                 1140             


Tyr Ile  Val Leu Val Lys Ile  Asp Asp Lys Lys Asn  Lys Leu Val 
    1145                 1150                 1155             


Lys Met  Pro Ile Arg Leu Ala  Glu Gln Val Asp Asn  Lys Thr Ile 
    1160                 1165                 1170             


Asp Leu  Gln Thr Trp Leu Glu  Glu Asn Val Pro His  Lys Lys Gly 
    1175                 1180                 1185             


Ile Gln  Val Leu Lys Thr Lys  Ile Pro Leu Gly Gln  Val Ile Tyr 
    1190                 1195                 1200             


Ser Lys  Lys Asn Gly Tyr Leu  Ala Leu Lys Ser Asp  Thr Glu Ile 
    1205                 1210                 1215             


Val Asn  Ala Gln Gln Leu Val  Leu Pro Tyr Gly Tyr  Val Ala Leu 
    1220                 1225                 1230             


Leu Thr  Leu Leu Gln Lys Met  Pro Glu Asn Lys Tyr  Asp Glu Val 
    1235                 1240                 1245             


Leu Ala  Cys Tyr Asp Asp Gly  Ile Leu Asp Asn Ile  Phe Val Ala 
    1250                 1255                 1260             


Ile Ile  Asp Lys Met Lys His  Phe Tyr Pro Tyr Tyr  Ser Gly Glu 
    1265                 1270                 1275             


Leu Lys  Thr Leu Ser Asp Asn  Ile Asp Asn Phe Lys  Glu Ser Pro 
    1280                 1285                 1290             


Asp Val  Asn Lys Ile Lys Thr  Leu Gln Gln Leu Leu  Leu Leu Leu 
    1295                 1300                 1305             


His Ala  Ser Ser Thr Thr Ala  Ser Ile Glu Phe Asn  Asn Val Lys 
    1310                 1315                 1320             


Lys Asn  Ser Leu Gly Arg Lys  Pro Asn Ser Ile Asn  Ile Gln Asn 
    1325                 1330                 1335             


Thr Asp  Leu Leu Ser Phe Ser  Ala Thr Gly Leu Tyr  Glu Ser Arg 
    1340                 1345                 1350             


Ile His  Ile Asp 
    1355         


<210>  940
<211>  1357
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  940

Met Ala Ala Lys Asn Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Trp Ala Ile Val Asp Asp Asn Tyr Gln Leu Met His Ala Lys 
            20                  25                  30          


Gly Lys Tyr Gly Tyr Gly Thr Arg Ile Tyr Ser Glu Gly Gln Thr Ala 
        35                  40                  45              


Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Asn Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Arg Leu Leu Gln Asp Phe Phe Glu Pro Tyr Ile Leu 
65                  70                  75                  80  


Pro Gln Asp Glu Ala Phe Phe Ile Arg Gln Lys Gln Ser Asn Leu Val 
                85                  90                  95      


Pro Gln Asp Thr Lys Lys Gln Tyr Pro Glu Ser Val Leu Phe Asp Asp 
            100                 105                 110         


Thr Ser Asp Lys Asp Phe Tyr Thr Glu Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Gln Ala Leu Met Thr Glu Asn Arg Gln Phe Asp Ile Arg Glu Val 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Asn Ala Pro Val Lys Thr Phe Lys Ser Gly Asn Leu Asp Leu Ala Ile 
                165                 170                 175     


Lys Phe Asn Glu Leu Asn Glu Glu Phe Lys Ser Val Tyr Pro Asp Leu 
            180                 185                 190         


Glu Leu Gln Phe Asn Leu Asp Gln Ile Asp Glu Ile Lys Lys Ile Leu 
        195                 200                 205             


Leu Asp Ser Thr Lys Ser Arg Phe Asp Arg Gln Gly Glu Ala Val Lys 
    210                 215                 220                 


Leu Ile Tyr Thr Val Ser Glu Val Lys Thr Ile Glu Lys Leu Gln Asn 
225                 230                 235                 240 


Lys Ile Val Lys Gln Phe Leu Ser Gly Ile Leu Gly Leu Asn Thr Arg 
                245                 250                 255     


Phe Asp Val Leu Thr Asn Thr Glu Ser Asp Asp Ser Met Trp Lys Phe 
            260                 265                 270         


Ser Leu Asp Ser Glu Ser Ser Asp Asp Ala Leu Asp Glu Ile Ile Ala 
        275                 280                 285             


Gln Leu Asp Asp Pro Ala Ile His Val Val Glu Leu Val Asn Glu Ile 
    290                 295                 300                 


His Ser Ser Ile Thr Leu Ala Gly Ile Val Pro Glu Gly Gln Gly Leu 
305                 310                 315                 320 


Ser Glu Ser Met Val Glu Lys Tyr Glu Ala His Lys Ala His Leu Gln 
                325                 330                 335     


Leu Leu Lys Ala Val Glu Lys Leu Val Asp Lys Lys Ala Ala Asp Lys 
            340                 345                 350         


Leu Glu Val Ala Tyr Asp Gln Tyr Ile Asn Gly Lys Asp Ser Lys Gln 
        355                 360                 365             


Phe Ile Lys Glu Asp Phe Tyr Lys Ala Val Gln Ser Glu Ile Lys Gly 
    370                 375                 380                 


Val Ala Gly Asp Glu Val Ala Glu Ile Asn Gln Leu Ile Glu Leu Asp 
385                 390                 395                 400 


Gln Phe Met Pro Lys Gln Arg Thr Lys Glu Asn Gly Ala Ile Pro His 
                405                 410                 415     


Gln Leu His Gln His Glu Leu Asp Val Ile Ile Glu Asn Gln Ser Lys 
            420                 425                 430         


Tyr Tyr Asp Trp Leu Gly Glu Lys Val Gly Asn Glu Tyr Lys Leu Asp 
        435                 440                 445             


Lys Leu Val Ser Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Val Asp 
    450                 455                 460                 


Thr Ala Ser Lys Glu Lys Lys Asp Glu Thr Arg Phe Ala Trp Met Val 
465                 470                 475                 480 


Arg Lys Asn Pro Glu Ser Thr Glu Ala Ile Thr Pro Trp Asn Phe Glu 
                485                 490                 495     


Asp Ile Val Asp Lys Asn Ala Ser Ala Glu Asn Phe Ile Lys Arg Met 
            500                 505                 510         


Thr Thr Lys Asp Lys Tyr Leu Leu Asn Glu Asp Val Leu Pro Lys Cys 
        515                 520                 525             


Ser Leu Leu Thr Gln Asp Phe Glu Val Tyr Asn Glu Leu Asn Asn Ile 
    530                 535                 540                 


Arg Val Asn Gly Glu Lys Phe Thr Ala Glu Gln Lys Gln Leu Ile His 
545                 550                 555                 560 


Asp Glu Leu Phe Thr Lys His Ala Ser Val Ser Ile Lys Gln Leu Lys 
                565                 570                 575     


Asp Phe Leu Val Ser Glu Leu Met Tyr Val Arg Asp Asn Leu Lys Val 
            580                 585                 590         


Glu Gly Leu Ala Asp Glu Lys Lys Leu Met Ser Ser Leu Ser Thr Gln 
        595                 600                 605             


Phe Asp Tyr Gln Lys Ile Ile Pro Asp Gln Ile Asn Asp Val Lys Tyr 
    610                 615                 620                 


Arg Glu Asp Ile Glu Gln Ile Ile Glu Trp Ser Thr Ile Phe Glu Asp 
625                 630                 635                 640 


Ala Ala Ile Phe Lys Thr Lys Leu Lys Glu Ile Asp Trp Leu Thr Asp 
                645                 650                 655     


Glu Gln Arg Asn Lys Leu Ala Asn Lys Arg Tyr Arg Gly Trp Gly Ser 
            660                 665                 670         


Leu Ser Lys Arg Leu Leu Ala Cys Leu Val Asp Glu Asn Gly Gln Arg 
        675                 680                 685             


Ile Ile Asp Ile Leu Arg Asp Ser Ser Asp Asn Phe Met Met Ile Val 
    690                 695                 700                 


Asn Arg Pro Glu Phe Lys Gln Ala Ile Phe Asp Glu Asn Ala Asn Leu 
705                 710                 715                 720 


Thr Glu Thr Asn Ser Lys Lys Asp Met Phe Glu Met Ile Asp Asn Phe 
                725                 730                 735     


Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Leu Leu Val 
            740                 745                 750         


Val Gln Asp Ile Gln Asn Ala Met Gly Gly Val Ala Pro Gln Lys Ile 
        755                 760                 765             


Phe Ile Glu Phe Ala Arg Glu Asp Glu Val Asn Gly Arg Arg Thr Asn 
    770                 775                 780                 


Ser Arg Tyr Ser Gln Ile Arg Glu Met Tyr Ser Lys Val Thr Asp Asp 
785                 790                 795                 800 


Val Ile Asn Lys Thr Val Arg Gly Glu Leu Glu Ala Asn Lys Gly Ser 
                805                 810                 815     


Leu Asn Lys Glu Lys Leu Phe Leu Tyr Phe Leu Gln Gly Gly Ile Asp 
            820                 825                 830         


Leu Tyr Ser Gly Lys Thr Ile Asn Ile Asp Asn Ile Ser Ser Tyr Asp 
        835                 840                 845             


Val Asp His Val Leu Pro Gln Ser Phe Ile Lys Asp Asp Ser Ile Asn 
    850                 855                 860                 


Asn Lys Val Leu Val Asn Ala Lys Gly Asn Arg Val Lys Asn Asp Asn 
865                 870                 875                 880 


Val Pro Met Glu Leu Phe Gly Ala Lys Met Tyr Pro Thr Trp Lys Arg 
                885                 890                 895     


Met Val Asp Thr Gly Ile Met Ser Lys Ala Lys Phe Lys Asn Leu Thr 
            900                 905                 910         


Leu Asn Pro Asp Asn Val Asp Lys Phe Lys Ala Glu Gly Phe Ile Asn 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys Leu Val Ala Gln Ile 
    930                 935                 940                 


Leu Asn Asp Lys Leu Gly Asp Asp Thr Lys Ile Val Ser Val Lys Ala 
945                 950                 955                 960 


Asn Leu Thr His Asp Met Arg Val Phe Phe Asp Phe Pro Lys Asn Arg 
                965                 970                 975     


Asn Val Asn Asn Tyr His His Ala Phe Asp Ala Tyr Leu Ser Ala Phe 
            980                 985                 990         


Val Gly Thr Tyr Leu Tyr Lys Arg  Tyr Pro Lys Leu Gln  Pro Tyr Phe 
        995                 1000                 1005             


Val Tyr  Gly Glu Phe Lys Lys  Met Asp Lys Leu Lys  Asn Met Thr 
    1010                 1015                 1020             


Ser Phe  Asp Phe Leu Arg Asp  Leu Lys His Thr Asp  Lys Val Val 
    1025                 1030                 1035             


Asp Lys  Asp Thr Gly Glu Ile  Ile Ala Asp Lys Leu  Ala Val Ile 
    1040                 1045                 1050             


Thr Asn  Met Asn Lys Ile Tyr  Asp Phe Lys Lys Val  Leu Val Thr 
    1055                 1060                 1065             


His Glu  Val His Glu Asn Ser  Gly Ala Met Tyr Asp  Gln Thr Leu 
    1070                 1075                 1080             


Tyr Pro  Ala Lys Leu Asp Lys  Ser Leu Gly Arg Lys  Gly Ala Lys 
    1085                 1090                 1095             


Gln Leu  Ile Pro Lys Lys Asp  Asp Met Pro Thr Ala  Leu Tyr Gly 
    1100                 1105                 1110             


Gly Tyr  Thr Gly Ser Lys Asp  Ala Phe Met Ser Ile  Val Arg Leu 
    1115                 1120                 1125             


Lys Lys  Lys Asp Glu Val Tyr  Tyr Lys Val Ile Gly  Ile Pro Val 
    1130                 1135                 1140             


Arg Ile  Ala Asp Ser Val Gln  His Gly Lys Glu Val  Asp Val Glu 
    1145                 1150                 1155             


Ala Leu  Thr Gln Phe Ile Glu  Thr Lys Phe Thr Lys  Glu Asn Leu 
    1160                 1165                 1170             


Asn Lys  Lys Thr Gly Glu Ile  Thr Arg Lys Val Glu  Trp Phe Glu 
    1175                 1180                 1185             


Leu Val  Leu Pro Lys Val Leu  Phe Gly Gln Ala Val  Phe Asp Gly 
    1190                 1195                 1200             


Gly Gln  Pro Phe Met Leu Gly  Ser Ser Thr Tyr Pro  His Asn Leu 
    1205                 1210                 1215             


Arg Glu  Leu Val Leu Thr Lys  Asp Ser Val Lys Lys  Ile Ala Gly 
    1220                 1225                 1230             


Lys Glu  Ser Asn Lys Ser Tyr  Leu Asp Ala Tyr Asp  Glu Ile Met 
    1235                 1240                 1245             


Glu Ala  Met Asn Lys Tyr Phe  Pro Leu Tyr Asp Thr  Asn Lys Phe 
    1250                 1255                 1260             


Arg Glu  Lys Val Ile Glu Gly  Arg Ser Lys Phe Ile  Asp Leu Pro 
    1265                 1270                 1275             


Asp Lys  Asp Glu Tyr Glu Asn  Asn Lys Leu Val Gln  Val Gly Lys 
    1280                 1285                 1290             


Ser Thr  Ile Ile Asp Arg Met  Phe Ile Gly Leu His  Ala Asn Ala 
    1295                 1300                 1305             


Ala Arg  Thr Asp Leu Lys Asn  Ile Gly Ile Thr Thr  Pro Phe Gly 
    1310                 1315                 1320             


Phe Met  Gln Gln Ala Ser Gly  Ile Lys Leu Thr Lys  Asp Thr Val 
    1325                 1330                 1335             


Val Leu  Phe Gln Ser Ser Thr  Gly Leu Phe Glu Arg  Lys Val Ala 
    1340                 1345                 1350             


Leu Lys  Asp Leu 
    1355         


<210>  941
<211>  1358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  941

Met Lys Glu Pro Tyr Gly Val Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Thr Val Val Asp Ala Ser Gly His Val Arg Lys Ile Lys Gly 
            20                  25                  30          


Gln Thr Gly Ile Gly Val Arg Leu Phe Lys Glu Gly Ala Ala Ala Ala 
        35                  40                  45              


Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Val Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Phe Phe Asp Gln Pro Ile Ser Lys 
65                  70                  75                  80  


Val Asp Ile Asn Phe Phe Ala Arg Arg Lys Tyr Ser Asp Val Ser Pro 
                85                  90                  95      


Arg Asp Pro Asn Tyr Asn Gly Leu Glu Lys Thr Leu Phe Asn Asp Arg 
            100                 105                 110         


Ser Asp Gln Asp Phe Tyr His Asp Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Glu Ala Leu Met Thr Gln His Arg Lys Phe Asp Val Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Arg Asn 
145                 150                 155                 160 


Asp Ala Ala Thr Ala Tyr Arg Ser Gly Thr Leu Asp Leu Gln Gln His 
                165                 170                 175     


Phe Glu Thr Leu Asn His Leu Phe Ser Gln Ala Asp Leu Glu Leu Asn 
            180                 185                 190         


Leu Asn Leu Thr Thr Asp Val Ala Leu Leu Asp Ser Ile Lys Gln Thr 
        195                 200                 205             


Leu Val Arg Thr Asp Ile Ser Arg Ser Asp Arg Gln Lys Leu Ile Met 
    210                 215                 220                 


Pro Leu Leu Ala Val Leu Thr Gly Ala Thr Thr Ala Glu Lys Lys Arg 
225                 230                 235                 240 


Gln Lys Ala Val Val Thr Glu Phe Ala Lys Ala Leu Val Gly Asn Lys 
                245                 250                 255     


Thr Lys Ile Asp Val Leu Thr Leu Thr Asp Ile Asp Ala Thr Glu Ala 
            260                 265                 270         


Lys Asp Trp Ala Phe Ser Leu Glu Glu Asn Gln Asp Lys Leu Pro Gly 
        275                 280                 285             


Ile Glu Asp Arg Phe Ser Glu Val Gly Gln Gln Ile Ile Asp Glu Val 
    290                 295                 300                 


Ile Arg Leu Tyr Ala Ser Val Asn Leu Ala Gln Leu Ile Pro Glu Gly 
305                 310                 315                 320 


Lys Arg Phe Ser Gln Ser Met Val Glu Lys Tyr Lys Arg His Gly Glu 
                325                 330                 335     


Asp Leu Lys Leu Leu Lys Ala Tyr Ile Arg Ser Gln Ser Asp Ala Lys 
            340                 345                 350         


Arg Gly Arg Ala Ile Arg Ala Thr Tyr Asp Gln Tyr Ile Asp Gly Val 
        355                 360                 365             


Lys Ser Lys Gln Val Thr Gln Glu Ala Phe Gln Lys Ala Leu Gly Lys 
    370                 375                 380                 


Phe Val Asp Ala Asp Val Asp Leu Asn Ser Trp Ala Ala Gln Ile Lys 
385                 390                 395                 400 


Gln Ala Ile Asp Ser Glu Gln Phe Met Pro Lys Leu Arg Thr Lys Gln 
                405                 410                 415     


Asn Gly Ala Ile Pro Tyr Gln Val Gln Gln Asn Glu Leu Asp Gln Ile 
            420                 425                 430         


Ile Ser Asn Gln Lys Glu Tyr Tyr Pro Trp Leu Gly Glu Glu Asn Pro 
        435                 440                 445             


Val Ile Lys Arg Arg Gly Lys Phe Pro Tyr Lys Leu Asp Glu Leu Phe 
    450                 455                 460                 


Gly Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr Lys Glu Met 
465                 470                 475                 480 


Gln Ala Ala Thr Ala Asn Ala Gly Phe Ala Trp Met Val Arg Lys Ala 
                485                 490                 495     


Asp Gly Pro Ile Thr Pro Trp Asn Phe Asp Asp Lys Val Asp Arg Val 
            500                 505                 510         


Ala Ser Ala Thr Ala Phe Ile Gln Arg Met Gln Thr Thr Asp Thr Tyr 
        515                 520                 525             


Leu Ile Gly Glu Glu Val Leu Pro Ala Arg Ser Leu Ile Tyr Gln Arg 
    530                 535                 540                 


Phe Met Val Leu Asn Glu Leu Asn Asn Ile Arg Val Asp Asp Gly Gln 
545                 550                 555                 560 


Leu Thr Pro Arg Gln Lys Gln Arg Leu Tyr Asn Gln Val Phe Lys Lys 
                565                 570                 575     


His Lys Arg Val Thr Ala Lys Asn Ile Gln Asp Asn Leu Val Ala Ala 
            580                 585                 590         


Arg Glu Phe Pro Thr Ala Pro Arg Ile Thr Gly Leu Ala Asp Pro Lys 
        595                 600                 605             


Val Phe Asn Ser Ser Leu Ser Thr Tyr His Asp Leu Lys Gln Val Leu 
    610                 615                 620                 


Pro Glu Ala Met Ser Asp Val Arg Lys Gln Ala Asp Ile Glu Lys Ile 
625                 630                 635                 640 


Ile Leu Trp Ser Thr Ile Phe Glu Asp Ser Thr Ile Phe Lys Gln Lys 
                645                 650                 655     


Leu Thr Glu Ile Ser Trp Leu Thr Ala Ala Gln Leu Lys Gln Leu Gly 
            660                 665                 670         


Gln Leu Arg Tyr Arg Gly Trp Gly Gln Leu Leu Arg Lys Leu Leu Thr 
        675                 680                 685             


Gly Leu Lys Asp Asp Asn Asn Arg Ser Ile Met Asp Gly Leu Trp Glu 
    690                 695                 700                 


Thr Asn Ala Asn Phe Met Gln Leu Arg Ser Gln Pro Ala Ile Glu Asn 
705                 710                 715                 720 


Gln Ile Lys Ser Ala Asn Gln Ala Asn Leu Thr Thr Ala Asp Leu Gln 
                725                 730                 735     


Asp Thr Ile Asn Glu Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile 
            740                 745                 750         


Arg Glu Val Met Val Val Leu Ala Asp Ile Lys Asp Ala Met His Gly 
        755                 760                 765             


Gln Thr Pro Asn Trp Ile Phe Val Glu Ala Ala Arg Gly Gly Gly Val 
    770                 775                 780                 


Ala Gly Arg Arg Thr Gln Ser Arg Ser Lys Gln Ile Val Ala Ala Tyr 
785                 790                 795                 800 


Gln Gly Thr Ala Gln Ala Ile Val Arg Asp Gln Val Gln Arg Glu Leu 
                805                 810                 815     


Lys Glu Lys Ile Lys Asn Lys Ala Asn Phe Asn Thr Arg Leu Val Leu 
            820                 825                 830         


Tyr Phe Leu Gln Asn Gly Arg Asp Leu Tyr Thr Gly Asp Ala Ile Asn 
        835                 840                 845             


Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Leu Pro Gln Ser 
    850                 855                 860                 


Leu Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Asn Ala Arg 
865                 870                 875                 880 


Ile Asn Arg Glu Lys Asn Asp Thr Phe Ala Ser Glu Lys Phe Gly Lys 
                885                 890                 895     


Lys Met Gly Ala Lys Trp Lys Glu Met His Arg Met Gly Leu Met Thr 
            900                 905                 910         


Lys Arg Lys Leu Asp His Leu Leu Met Arg Pro Asp Glu Ile Ser Lys 
        915                 920                 925             


Tyr Ala Thr Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val 
    930                 935                 940                 


Ile Lys Leu Val Glu Glu Leu Ile Asn Ala Glu Tyr Pro Ser Thr Ala 
945                 950                 955                 960 


Ile Val Ser Val Lys Ala Asn Leu Thr His Gln Phe Arg Gln Thr Phe 
                965                 970                 975     


Asp Phe Pro Lys Ile Arg Glu Val Asn Asp Tyr His His Ala Phe Asp 
            980                 985                 990         


Ala Ala Leu Thr Ala Phe Ile Gly  Thr Tyr Leu Leu Lys  Arg Tyr Pro 
        995                 1000                 1005             


Lys Leu  Glu Arg Phe Phe Val  Tyr Gly Lys Phe Ala  Lys Gln Pro 
    1010                 1015                 1020             


Val Asn  Leu Thr Arg Phe Asn  Val Ile Arg Lys Leu  Ala Lys Thr 
    1025                 1030                 1035             


Asp Thr  Pro Ile Thr Asp Ile  Asp Thr Gly Glu Val  Leu Trp Asp 
    1040                 1045                 1050             


Lys Leu  Thr Asp Ile Lys Tyr  Phe Glu Thr Leu Tyr  Asp Tyr Lys 
    1055                 1060                 1065             


Arg Leu  Leu Val Thr Arg Glu  Val Ser Glu Asn Tyr  Gly Ala Met 
    1070                 1075                 1080             


Phe Asp  Gln Thr Leu Leu Lys  Ala Ser Asp Asn Gly  Lys Arg Leu 
    1085                 1090                 1095             


Ile Arg  Lys Lys Gln Ser Phe  Asp Thr Ala Ile Tyr  Gly Gly Tyr 
    1100                 1105                 1110             


Thr Gly  Lys Thr Leu Ala Tyr  Met Ala Ile Val Lys  Val Ser Thr 
    1115                 1120                 1125             


Lys Lys  Asp Val Gln Tyr Gln  Val Val Gly Val Pro  Thr Thr Met 
    1130                 1135                 1140             


Val Thr  Gln Ile Glu Gln Leu  Glu Gln Gln Gly Met  Thr Asn Lys 
    1145                 1150                 1155             


Gln Ala  Val Thr Lys Val Ile  Glu Pro Leu Ile Ala  Ala Asn Asn 
    1160                 1165                 1170             


Lys Lys  Met Leu Thr Tyr Glu  Val Leu Leu Pro Lys  Val Arg Phe 
    1175                 1180                 1185             


Glu Gln  Val Val Arg Asp Arg  Ile Lys Gly Gln Thr  His Arg Phe 
    1190                 1195                 1200             


Ala Leu  Gly Thr Asp Thr Tyr  Tyr His Asn Ile Gln  Glu Leu Tyr 
    1205                 1210                 1215             


Leu Pro  Leu Thr Leu Gln Arg  Ala Phe Leu Gly Lys  Gln Asn Glu 
    1220                 1225                 1230             


Ser Glu  Val Gln Leu Asn Gln  Asn Leu Leu Thr Val  Phe Asp Ala 
    1235                 1240                 1245             


Thr Leu  Ala Gln Val Asn Arg  Tyr Phe Pro Leu Tyr  Glu Met Asn 
    1250                 1255                 1260             


Lys Phe  Lys Lys Ile Leu Asn  Ser Ala Arg Asp Gln  Ile Glu Leu 
    1265                 1270                 1275             


Leu Glu  Ala Lys Asn Gln Phe  Val Lys Asn Lys Leu  Val Leu Gly 
    1280                 1285                 1290             


Lys Gln  Gly Ile Leu Thr Asn  Met Phe Ile Gly Leu  His Ala Asn 
    1295                 1300                 1305             


Ala Ser  Tyr Gly Asp Leu Lys  Val Leu Gly Val Lys  Ile Asp Phe 
    1310                 1315                 1320             


Gly Lys  Leu Gln Val Pro Gly  Gly Ile Lys Leu Thr  Gly Asp Ala 
    1325                 1330                 1335             


Glu Ile  Ile Tyr Gln Ser Pro  Thr Gly Leu Phe Glu  Arg Lys Ile 
    1340                 1345                 1350             


Ala Leu  Lys Asp Leu 
    1355             


<210>  942
<211>  1358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  942

Met Asn Glu Pro Tyr Gly Val Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Thr Val Val Asp Met Asn Gly Arg Val Arg Lys Val Lys Gly 
            20                  25                  30          


Lys Thr Ala Leu Gly Ala Arg Leu Phe Lys Glu Gly Ala Thr Ala Glu 
        35                  40                  45              


Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Val Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Phe Phe Asp Gln Pro Ile Ser Lys 
65                  70                  75                  80  


Ile Asp Pro Asn Phe Phe Ala Arg Arg Lys Tyr Ser Asp Ile Ser Pro 
                85                  90                  95      


Arg Asp Pro Asn Tyr Asn Gly Leu Ala Lys Thr Leu Phe Asn Asp Arg 
            100                 105                 110         


Thr Asp Gln Glu Phe Tyr His Asp Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Asp Lys Leu Met Thr Ser Asp Arg Lys Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Arg Thr 
145                 150                 155                 160 


Gly Pro Ala Ser Gln Tyr Gly Ser Ala Ser Leu His Leu Ala Thr Ser 
                165                 170                 175     


Phe Gln Lys Leu Asn Asp Leu Phe Ala Gln Ser Glu Glu Ser Leu Asn 
            180                 185                 190         


Phe Lys Leu Val Thr Asp Glu Ala Gln Leu Gln Gln Ile Gln Gln Ile 
        195                 200                 205             


Leu Val Arg Thr Asp Leu Ser Arg Ser Glu Gln Gln Arg Gln Ile Trp 
    210                 215                 220                 


Pro Leu Met Val Val Leu Met Gly Ala Thr Ala Ala Glu Lys Lys Arg 
225                 230                 235                 240 


Gln Lys Asp Phe Ala Lys Glu Leu Ala Asn Ala Leu Val Gly Leu Lys 
                245                 250                 255     


Ala Lys Leu Asn Val Val Thr Leu Thr Asn Val Asp Thr Thr Val Val 
            260                 265                 270         


Lys Asp Trp Thr Phe Thr Leu Glu Glu Ser Gln Asp Lys Leu Pro Glu 
        275                 280                 285             


Ile Glu Glu Gln Leu Ser Glu Val Gly Gln Gln Ile Met Asp Glu Val 
    290                 295                 300                 


Ile Gln Leu Tyr Ala Ser Val Asn Leu Ala Gln Leu Ile Pro Ala Gly 
305                 310                 315                 320 


Lys Arg Phe Ser Gln His Met Val Glu Lys Tyr Asn His His Glu Lys 
                325                 330                 335     


Asn Leu Glu Leu Leu Lys Ala Tyr Ile His Ser Gln Ser Asp Ser Lys 
            340                 345                 350         


Arg Gly Arg Glu Ile Arg Ala Thr Tyr Asp Arg Tyr Ile Asp Gly Val 
        355                 360                 365             


Lys Ser Lys Pro Val Thr Gln Glu Met Phe Tyr Lys Asp Leu Met Lys 
    370                 375                 380                 


Tyr Val Glu Ala Asp Ala Thr Ser Asn His Leu Ala Ala Glu Ile Lys 
385                 390                 395                 400 


Asp Glu Ile Asp Ser Glu Gln Phe Met Pro Lys Leu Arg Thr Lys Gln 
                405                 410                 415     


Asn Gly Ser Ile Pro Tyr Gln Val Gln Gln Tyr Glu Leu Asp Gln Ile 
            420                 425                 430         


Ile Ser His Gln Lys Lys Tyr Tyr Pro Trp Leu Gly Glu Glu Asn Pro 
        435                 440                 445             


Val Ala Glu Arg Arg Gly Lys Phe Pro Tyr Lys Leu Asp Glu Leu Val 
    450                 455                 460                 


Gly Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr Lys Glu Asp 
465                 470                 475                 480 


Gln Gln Ala Thr Ser Gly Ala Gly Phe Ala Trp Met Val Arg Lys Ala 
                485                 490                 495     


Asp Gly Pro Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Ile 
            500                 505                 510         


Ala Ser Ala Thr Ala Phe Ile Gln Arg Met Gln Thr Thr Asp Thr Tyr 
        515                 520                 525             


Leu Ile Gly Glu Asp Val Leu Pro Ala Arg Ser Leu Ile Tyr Gln Arg 
    530                 535                 540                 


Phe Met Val Leu Asn Glu Leu Asn Asn Met Arg Val Glu Asp Gln Lys 
545                 550                 555                 560 


Leu Ala Pro Arg Gln Lys Gln Arg Leu Tyr Asn Gln Val Phe Lys Gln 
                565                 570                 575     


His Gln His Val Ser Val Lys Asn Ile Gln Gln Asn Leu Met Asp Ala 
            580                 585                 590         


Gly Glu Tyr Arg Glu Thr Pro Lys Ile Thr Gly Leu Ala Asp Pro Lys 
        595                 600                 605             


Gly Phe Asn Ser Ser Leu Ser Thr Tyr His Asp Phe Lys Lys Ile Leu 
    610                 615                 620                 


Pro Glu Ala Ile Ala Asp Glu His Lys Arg Ala Asp Ile Glu Lys Ile 
625                 630                 635                 640 


Ile Leu Trp Ser Thr Thr Phe Glu Asp Ser Ala Ile Phe Lys Gln Lys 
                645                 650                 655     


Leu Glu Glu Val Ala Trp Leu Thr Asp Ala Gln Arg Lys Gln Leu Ser 
            660                 665                 670         


Gly Leu Arg Tyr Arg Gly Trp Gly Gln Leu Ser His Lys Leu Leu Thr 
        675                 680                 685             


Ala Phe Lys Asp Asp Asn Gly Arg Ser Val Met Asp Gly Leu Trp Glu 
    690                 695                 700                 


Thr Ser Asp Asn Phe Met Gln Leu Arg Lys Gln Pro Ala Ile Glu Thr 
705                 710                 715                 720 


Gln Ile Lys Glu Ala Asn Gln Ala Asn Leu Thr Asp Ala Asp Ile Gln 
                725                 730                 735     


Asp Thr Ile Asn Glu Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile 
            740                 745                 750         


Arg Glu Val Met Leu Val Leu Asp Asp Ile Lys Asn Ala Met His Gly 
        755                 760                 765             


Gln Thr Pro Ser Trp Ile Phe Ile Glu Ala Ala Arg Gly Gly Gly Val 
    770                 775                 780                 


Ala Gly Arg Arg Thr Gln Ser Arg Ser Lys Gln Ile Val Glu Ala Tyr 
785                 790                 795                 800 


Lys Ser Thr Ala Lys Glu Ile Val Ser Glu Lys Val Gln His Glu Leu 
                805                 810                 815     


Asn Glu Lys Ile Lys Ala Lys Ala Asp Phe Asn Thr Arg Leu Val Leu 
            820                 825                 830         


Tyr Phe Leu Gln Asn Gly Arg Asp Leu Tyr Thr Asn Glu Ala Ile Asn 
        835                 840                 845             


Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Leu Pro Gln Ser 
    850                 855                 860                 


Leu Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ala Arg 
865                 870                 875                 880 


Ile Asn Arg Glu Lys Asn Asp Thr Phe Ala Ser Glu Lys Phe Gly Arg 
                885                 890                 895     


Lys Met Gly Ala Gln Trp Arg Glu Leu His Arg Asn Gly Leu Met Thr 
            900                 905                 910         


Gln Arg Lys Leu Lys His Leu Leu Met Arg Pro Asp Glu Ile Ser Lys 
        915                 920                 925             


His Ala Thr Asp Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val 
    930                 935                 940                 


Ile Lys Leu Val Glu Glu Leu Ile Ser Ser Glu Tyr Pro Ser Ala Ser 
945                 950                 955                 960 


Ile Val Ala Val Lys Ala Asn Leu Thr His Gln Phe Arg Gln Thr Phe 
                965                 970                 975     


Asn Phe Pro Lys Leu Arg Glu Val Asn Asp Tyr His His Ala Phe Asp 
            980                 985                 990         


Ala Ser Leu Thr Ala Phe Ile Gly  Met Tyr Leu Leu Lys  Gln Tyr Pro 
        995                 1000                 1005             


Lys Leu  Glu Arg Phe Phe Val  Tyr Gly Lys Phe Ala  Lys Gln Pro 
    1010                 1015                 1020             


Ile Asn  Leu Thr Arg Phe Asn  Val Ile Arg Lys Leu  Ala Val Ala 
    1025                 1030                 1035             


Glu Glu  Pro Ile Ala Asn Ile  Glu Thr Gly Glu Ile  Leu Trp Asp 
    1040                 1045                 1050             


Lys Thr  Ala Asp Ile Lys Cys  Phe Glu Lys Leu Tyr  Asn Tyr Lys 
    1055                 1060                 1065             


Arg Leu  Leu Val Thr His Glu  Val Arg Glu Asn Tyr  Gly Ala Met 
    1070                 1075                 1080             


Phe Asp  Gln Thr Ile Phe Lys  Ala Thr Asp Ser Arg  Lys Arg Leu 
    1085                 1090                 1095             


Ile Gln  Lys Lys Lys Gly Phe  Ala Thr Glu Thr Tyr  Gly Gly Tyr 
    1100                 1105                 1110             


Thr Gly  Arg Thr Leu Ala Tyr  Leu Ala Ile Val Lys  Val Val Thr 
    1115                 1120                 1125             


Asn Lys  Asp Thr Gln Tyr Gln  Val Val Gly Ile Pro  Thr Thr Met 
    1130                 1135                 1140             


Val Thr  Gln Ile Gln Gln Leu  Glu Ala Ser Gly Leu  Thr Ser Lys 
    1145                 1150                 1155             


Gln Ala  Val Thr Lys Ala Ile  Gly Ala Met Ile Asn  Glu Asn Asn 
    1160                 1165                 1170             


Lys Lys  Leu Gln Ser Tyr Glu  Val Leu Leu Pro Lys  Val Arg Phe 
    1175                 1180                 1185             


Glu Gln  Val Val Arg Asp Glu  Val Lys Gly His Ile  His Arg Phe 
    1190                 1195                 1200             


Ala Leu  Gly Thr Asp Thr Tyr  Tyr His Asn Ile Gln  Glu Leu Tyr 
    1205                 1210                 1215             


Leu Pro  Leu Thr Ile Gln Arg  Ala Phe Leu Gly Lys  Arg Asn Asp 
    1220                 1225                 1230             


Thr Asp  Ala Gln Thr Glu Gln  Asn Leu Leu Thr Val  Phe Glu Thr 
    1235                 1240                 1245             


Thr Leu  Ala Gln Val Asn Arg  Tyr Phe Pro Leu Tyr  Glu Met Asn 
    1250                 1255                 1260             


Lys Phe  Lys Gln Ile Leu Asn  Ser Ala Gln Glu Lys  Phe Ala Ala 
    1265                 1270                 1275             


Leu Pro  Thr Lys Asn Lys Thr  Val Lys Asn Lys Leu  Val Thr Gly 
    1280                 1285                 1290             


Lys Asp  Gly Val Leu Asn Asn  Leu Phe Ile Gly Leu  His Ala Asn 
    1295                 1300                 1305             


Ala Ser  Leu Gly Asp Leu Lys  Ile Leu Gly Met Lys  Thr Gly Phe 
    1310                 1315                 1320             


Gly Lys  Leu Gln Val Pro Gly  Gly Leu Lys Leu Thr  Asp Gln Ala 
    1325                 1330                 1335             


Glu Ile  Ile Tyr Gln Ser Pro  Thr Gly Leu Phe Glu  Arg Lys Val 
    1340                 1345                 1350             


Ala Leu  Lys Asp Leu 
    1355             


<210>  943
<211>  1358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  943

Met Asn Glu Pro Tyr Gly Val Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Thr Val Val Asp Met Asn Gly Arg Val Arg Lys Val Lys Gly 
            20                  25                  30          


Lys Thr Ala Leu Gly Ala Arg Leu Phe Lys Glu Gly Ala Thr Ala Glu 
        35                  40                  45              


Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Val Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Phe Phe Asp Gln Pro Ile Ser Lys 
65                  70                  75                  80  


Ile Asp Pro Asn Phe Phe Ala Arg Arg Lys Tyr Ser Asp Ile Ser Pro 
                85                  90                  95      


Arg Asp Pro Asn Tyr Asn Gly Leu Ala Lys Thr Leu Phe Asn Asp Arg 
            100                 105                 110         


Thr Asp Gln Glu Phe Tyr His Asp Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Asp Lys Leu Met Thr Ser Asp Arg Lys Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Arg Thr 
145                 150                 155                 160 


Gly Pro Ala Ser Gln Tyr Gly Ser Ala Ser Leu His Leu Ala Thr Ser 
                165                 170                 175     


Phe Gln Lys Leu Asn Asp Leu Phe Ala Gln Ser Glu Glu Ser Leu Asn 
            180                 185                 190         


Phe Lys Leu Val Thr Asp Glu Ala Gln Leu Gln Gln Ile Gln Gln Ile 
        195                 200                 205             


Leu Val Arg Thr Asp Leu Ser Arg Ser Glu Gln Gln Arg Gln Ile Trp 
    210                 215                 220                 


Pro Leu Met Val Val Leu Met Gly Ala Thr Ala Ala Glu Lys Lys Arg 
225                 230                 235                 240 


Gln Lys Asp Phe Ala Lys Glu Leu Ala Asn Ala Leu Val Gly Leu Lys 
                245                 250                 255     


Ala Lys Leu Asn Val Val Thr Leu Thr Asn Val Asp Thr Thr Val Val 
            260                 265                 270         


Lys Asp Trp Thr Phe Thr Leu Glu Glu Ser Gln Asp Lys Leu Pro Glu 
        275                 280                 285             


Ile Glu Glu Gln Leu Ser Glu Val Gly Gln Gln Ile Met Asp Glu Val 
    290                 295                 300                 


Ile Gln Leu Tyr Ala Ser Val Asn Leu Ala Gln Leu Ile Pro Ala Gly 
305                 310                 315                 320 


Lys Arg Phe Ser Gln His Met Val Glu Lys Tyr Asn His His Glu Lys 
                325                 330                 335     


Asn Leu Glu Leu Leu Lys Ala Tyr Ile His Ser Gln Ser Asp Ser Lys 
            340                 345                 350         


Arg Gly Arg Glu Ile Arg Ala Thr Tyr Asp Arg Tyr Ile Asp Gly Val 
        355                 360                 365             


Lys Ser Lys Pro Val Thr Gln Glu Met Phe Tyr Lys Asp Leu Met Lys 
    370                 375                 380                 


Tyr Val Glu Ala Asp Ala Thr Ser Asn His Leu Ala Ala Glu Ile Lys 
385                 390                 395                 400 


Asp Glu Ile Asp Ser Glu Gln Phe Met Pro Lys Leu Arg Thr Lys Gln 
                405                 410                 415     


Asn Gly Ser Ile Pro Tyr Gln Val Gln Gln Tyr Glu Leu Asp Gln Ile 
            420                 425                 430         


Ile Ser His Gln Lys Lys Tyr Tyr Pro Trp Leu Gly Glu Glu Asn Pro 
        435                 440                 445             


Val Ala Glu Arg Arg Gly Lys Phe Pro Tyr Lys Leu Asp Glu Leu Val 
    450                 455                 460                 


Gly Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr Lys Glu Asp 
465                 470                 475                 480 


Gln Gln Ala Thr Ser Gly Ala Gly Phe Ala Trp Met Val Arg Lys Ala 
                485                 490                 495     


Asp Gly Pro Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Ile 
            500                 505                 510         


Ala Ser Ala Thr Ala Phe Ile Gln Arg Met Gln Thr Thr Asp Thr Tyr 
        515                 520                 525             


Leu Ile Gly Glu Asp Val Leu Pro Ala Arg Ser Leu Ile Tyr Gln Arg 
    530                 535                 540                 


Phe Met Val Leu Asn Glu Leu Asn Asn Met Arg Val Glu Asp Gln Lys 
545                 550                 555                 560 


Leu Ala Pro Arg Gln Lys Gln Arg Leu Tyr Asn Gln Val Phe Lys Gln 
                565                 570                 575     


His Gln His Val Ser Val Lys Asn Ile Gln Gln Asn Leu Met Asp Ala 
            580                 585                 590         


Gly Glu Tyr Arg Glu Thr Pro Lys Ile Thr Gly Leu Ala Asp Pro Lys 
        595                 600                 605             


Gly Phe Asn Ser Ser Leu Ser Thr Tyr His Asp Phe Lys Lys Ile Leu 
    610                 615                 620                 


Pro Glu Ala Ile Ala Asp Glu His Lys Arg Ala Asp Ile Glu Lys Ile 
625                 630                 635                 640 


Ile Leu Trp Ser Thr Thr Phe Glu Asp Ser Ala Ile Phe Lys Gln Lys 
                645                 650                 655     


Leu Glu Glu Val Ala Trp Leu Thr Asp Ala Gln Arg Lys Gln Leu Ser 
            660                 665                 670         


Gly Leu Arg Tyr Arg Gly Trp Gly Gln Leu Ser His Lys Leu Leu Thr 
        675                 680                 685             


Ala Phe Lys Asp Asp Asn Gly Arg Ser Val Met Asp Gly Leu Trp Glu 
    690                 695                 700                 


Thr Ser Asp Asn Phe Met Gln Leu Arg Lys Gln Pro Ala Ile Glu Thr 
705                 710                 715                 720 


Gln Ile Lys Glu Ala Asn Gln Ala Asn Leu Thr Asp Ala Asp Ile Gln 
                725                 730                 735     


Asp Thr Ile Asn Glu Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile 
            740                 745                 750         


Arg Glu Val Met Leu Val Leu Asp Asp Ile Lys Asn Ala Met His Gly 
        755                 760                 765             


Gln Thr Pro Ser Trp Ile Phe Ile Glu Ala Ala Arg Gly Gly Gly Val 
    770                 775                 780                 


Ala Gly Arg Arg Thr Gln Ser Arg Ser Lys Gln Ile Val Glu Ala Tyr 
785                 790                 795                 800 


Lys Ser Thr Ala Lys Glu Ile Val Ser Glu Lys Val Gln His Glu Leu 
                805                 810                 815     


Asn Glu Lys Ile Lys Ala Lys Ala Asp Phe Asn Thr Arg Leu Val Leu 
            820                 825                 830         


Tyr Phe Leu Gln Asn Gly Arg Asp Leu Tyr Thr Asn Glu Ala Ile Asn 
        835                 840                 845             


Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Leu Pro Gln Ser 
    850                 855                 860                 


Leu Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ala Arg 
865                 870                 875                 880 


Ile Asn Arg Glu Lys Asn Asp Thr Phe Ala Ser Glu Lys Phe Gly Arg 
                885                 890                 895     


Lys Met Gly Ala Gln Trp Arg Glu Leu His Arg Asn Gly Leu Met Thr 
            900                 905                 910         


Gln Arg Lys Leu Lys His Leu Leu Met Arg Pro Asp Glu Ile Ser Lys 
        915                 920                 925             


His Ala Thr Asp Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val 
    930                 935                 940                 


Ile Lys Leu Val Glu Glu Leu Ile Ser Ser Glu Tyr Pro Ser Ala Ser 
945                 950                 955                 960 


Ile Val Ala Val Lys Ala Asn Leu Thr His Gln Phe Arg Gln Thr Phe 
                965                 970                 975     


Asn Phe Pro Lys Leu Arg Glu Val Asn Asp Tyr His His Ala Phe Asp 
            980                 985                 990         


Ala Ser Leu Thr Ala Phe Ile Gly  Met Tyr Leu Leu Lys  Gln Tyr Pro 
        995                 1000                 1005             


Lys Leu  Glu Arg Phe Phe Val  Tyr Gly Lys Phe Ala  Lys Gln Pro 
    1010                 1015                 1020             


Ile Asn  Leu Thr Arg Phe Asn  Val Ile Arg Lys Leu  Ala Val Ala 
    1025                 1030                 1035             


Glu Glu  Pro Ile Ala Asn Ile  Glu Thr Gly Glu Ile  Leu Trp Asp 
    1040                 1045                 1050             


Lys Thr  Ala Asp Ile Lys Cys  Phe Glu Lys Leu Tyr  Asn Tyr Lys 
    1055                 1060                 1065             


Arg Leu  Leu Val Thr His Glu  Val Arg Glu Asn Tyr  Gly Ala Met 
    1070                 1075                 1080             


Phe Asp  Gln Thr Ile Phe Lys  Ala Thr Asp Ser Arg  Lys Arg Leu 
    1085                 1090                 1095             


Ile Gln  Lys Lys Lys Gly Phe  Ala Thr Glu Thr Tyr  Gly Gly Tyr 
    1100                 1105                 1110             


Thr Gly  Arg Thr Leu Ala Tyr  Leu Ala Ile Val Lys  Val Val Thr 
    1115                 1120                 1125             


Asn Lys  Asp Thr Gln Tyr Gln  Val Val Gly Ile Pro  Thr Thr Met 
    1130                 1135                 1140             


Val Thr  Gln Ile Gln Gln Leu  Glu Ala Ser Gly Leu  Thr Ser Lys 
    1145                 1150                 1155             


Gln Ala  Val Thr Lys Ala Ile  Gly Ala Met Ile Asn  Glu Asn Asn 
    1160                 1165                 1170             


Lys Lys  Leu Gln Ser Tyr Glu  Val Leu Leu Pro Lys  Val Arg Phe 
    1175                 1180                 1185             


Glu Gln  Val Val Arg Asp Glu  Val Lys Gly His Ile  His Arg Phe 
    1190                 1195                 1200             


Ala Leu  Gly Thr Asp Thr Tyr  Tyr His Asn Ile Gln  Glu Leu Tyr 
    1205                 1210                 1215             


Leu Pro  Leu Thr Ile Gln Arg  Ala Phe Leu Gly Lys  Arg Asn Asp 
    1220                 1225                 1230             


Thr Asp  Ala Gln Thr Glu Gln  Asn Leu Leu Thr Val  Phe Glu Thr 
    1235                 1240                 1245             


Thr Leu  Ala Gln Val Asn Arg  Tyr Phe Pro Leu Tyr  Glu Met Asn 
    1250                 1255                 1260             


Lys Phe  Lys Gln Ile Leu Asn  Ser Ala Gln Glu Lys  Phe Ala Ala 
    1265                 1270                 1275             


Leu Pro  Thr Lys Asn Lys Thr  Val Lys Asn Lys Leu  Val Thr Gly 
    1280                 1285                 1290             


Lys Asp  Gly Val Leu Asn Asn  Leu Phe Ile Gly Leu  His Ala Asn 
    1295                 1300                 1305             


Ala Ser  Leu Gly Asp Leu Lys  Ile Leu Gly Met Lys  Ile Gly Phe 
    1310                 1315                 1320             


Gly Lys  Leu Gln Val Pro Gly  Gly Leu Lys Leu Thr  Asp Gln Ala 
    1325                 1330                 1335             


Glu Ile  Ile Tyr Gln Ser Pro  Thr Gly Leu Phe Glu  Arg Lys Val 
    1340                 1345                 1350             


Ala Leu  Lys Asp Leu 
    1355             


<210>  944
<211>  1358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  944

Met Lys Glu Pro Tyr Gly Val Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Thr Val Val Asp Ala Ser Gly His Val Arg Lys Ile Lys Gly 
            20                  25                  30          


Gln Thr Gly Ile Gly Val Arg Leu Phe Lys Glu Gly Ala Ala Ala Ala 
        35                  40                  45              


Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Val Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Phe Phe Asp Gln Pro Ile Ser Lys 
65                  70                  75                  80  


Val Asp Ile Asn Phe Phe Ala Arg Arg Lys Tyr Ser Asp Ile Ser Pro 
                85                  90                  95      


Arg Asp Pro Asn Tyr Asn Gly Leu Glu Lys Thr Leu Phe Asn Asp Arg 
            100                 105                 110         


Ser Asp Gln Asp Phe Tyr His Asp Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Glu Ala Leu Met Thr Gln His Arg Lys Phe Asp Val Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Arg Asn 
145                 150                 155                 160 


Asp Ala Ala Thr Ala Tyr Arg Ser Gly Thr Leu Asp Leu Gln Gln His 
                165                 170                 175     


Phe Glu Thr Leu Asn His Leu Phe Ser Gln Ala Asp Leu Glu Leu Asn 
            180                 185                 190         


Leu Asn Leu Thr Thr Asp Val Ala Leu Leu Asp Ser Ile Lys Gln Thr 
        195                 200                 205             


Leu Val Arg Thr Asp Ile Ser Arg Ser Asp Arg Gln Lys Leu Ile Met 
    210                 215                 220                 


Pro Leu Leu Ala Val Leu Thr Gly Ala Thr Thr Ala Glu Lys Lys Arg 
225                 230                 235                 240 


Gln Lys Ala Val Val Thr Glu Phe Ala Lys Ala Leu Val Gly Asn Lys 
                245                 250                 255     


Thr Lys Ile Asp Val Leu Thr Leu Thr Asp Ile Asp Ala Thr Glu Ala 
            260                 265                 270         


Lys Asp Trp Ala Phe Ser Leu Glu Glu Asn Gln Asp Lys Leu Pro Gly 
        275                 280                 285             


Ile Glu Asp Arg Phe Ser Glu Val Gly Gln Gln Ile Ile Asp Glu Val 
    290                 295                 300                 


Ile Arg Leu Tyr Ala Ser Val Asn Leu Ala Gln Leu Ile Pro Glu Gly 
305                 310                 315                 320 


Lys Arg Phe Ser Gln Ser Met Val Glu Lys Tyr Lys Arg His Gly Glu 
                325                 330                 335     


Asp Leu Lys Leu Leu Lys Ala Tyr Ile Arg Ser Gln Ser Asp Ala Lys 
            340                 345                 350         


Arg Gly Arg Ala Ile Arg Ala Thr Tyr Asp Gln Tyr Ile Asp Gly Val 
        355                 360                 365             


Lys Ser Lys Gln Val Thr Gln Glu Ala Phe Gln Lys Ala Leu Gly Lys 
    370                 375                 380                 


Phe Val Asp Ala Asp Val Asp Leu Asn Ser Trp Ala Ala Gln Ile Lys 
385                 390                 395                 400 


Gln Ala Ile Asp Ser Glu Gln Phe Met Pro Lys Leu Arg Thr Lys Gln 
                405                 410                 415     


Asn Gly Ala Ile Pro Tyr Gln Val Gln Gln Asn Glu Leu Asp Gln Ile 
            420                 425                 430         


Ile Asn Asn Gln Lys Glu Tyr Tyr Pro Trp Leu Gly Glu Glu Asn Pro 
        435                 440                 445             


Val Ile Lys Arg Arg Gly Lys Phe Pro Tyr Lys Leu Asp Glu Leu Val 
    450                 455                 460                 


Gly Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr Lys Glu Met 
465                 470                 475                 480 


Gln Ala Ala Thr Ala Asn Ala Gly Phe Ala Trp Met Val Arg Lys Ala 
                485                 490                 495     


Asp Gly Pro Ile Thr Pro Trp Asn Phe Asp Asp Lys Val Asp Arg Val 
            500                 505                 510         


Ala Ser Ala Thr Ala Phe Ile Gln Arg Met Gln Thr Thr Asp Thr Tyr 
        515                 520                 525             


Leu Ile Gly Glu Glu Val Leu Pro Ala Arg Ser Leu Ile Tyr Gln Arg 
    530                 535                 540                 


Phe Met Val Leu Asn Glu Leu Asn Asn Ile Arg Val Asp Asp Gly Gln 
545                 550                 555                 560 


Leu Thr Pro Arg Gln Lys Gln Arg Leu Tyr Asn Gln Val Phe Lys Lys 
                565                 570                 575     


His Lys Arg Val Thr Ala Lys Asn Ile Gln Asp Asn Leu Val Ala Ala 
            580                 585                 590         


Gly Glu Phe Pro Thr Ala Pro Arg Ile Thr Gly Leu Ala Asp Pro Lys 
        595                 600                 605             


Val Phe Asn Ser Ser Leu Ser Thr Tyr His Asp Leu Lys Gln Val Leu 
    610                 615                 620                 


Pro Glu Ala Met Ser Asp Val Arg Lys Gln Ala Asp Ile Glu Lys Ile 
625                 630                 635                 640 


Ile Leu Trp Ser Thr Ile Phe Glu Asp Ser Thr Ile Phe Lys Gln Lys 
                645                 650                 655     


Leu Thr Glu Ile Ser Trp Leu Thr Ala Ala Gln Leu Lys Gln Leu Gly 
            660                 665                 670         


Gln Leu Arg Tyr Arg Gly Trp Gly Gln Leu Ser Arg Lys Leu Leu Thr 
        675                 680                 685             


Gly Leu Lys Asp Asp Asn Asn Arg Ser Ile Met Asp Gly Leu Trp Glu 
    690                 695                 700                 


Thr Asn Ala Asn Phe Met Gln Leu Arg Ser Gln Pro Ala Ile Glu Asn 
705                 710                 715                 720 


Gln Ile Lys Ser Ala Asn Gln Ala Asn Leu Thr Thr Ala Asp Leu Gln 
                725                 730                 735     


Asp Thr Ile Asn Glu Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile 
            740                 745                 750         


Arg Glu Val Met Val Val Leu Ala Asp Ile Lys Asp Ala Met His Gly 
        755                 760                 765             


Gln Thr Pro Asn Trp Ile Phe Val Glu Ala Ala Arg Gly Gly Gly Val 
    770                 775                 780                 


Ala Gly Arg Arg Thr Gln Ser Arg Ser Lys Gln Ile Val Ala Ala Tyr 
785                 790                 795                 800 


Gln Gly Thr Ala Gln Val Ile Val Ser Asp Gln Val Gln Arg Glu Leu 
                805                 810                 815     


Lys Glu Lys Ile Lys Asn Lys Ala Asn Phe Asn Thr Arg Leu Val Leu 
            820                 825                 830         


Tyr Phe Leu Gln Asn Gly Arg Asp Leu Tyr Thr Gly Asp Ala Ile Asn 
        835                 840                 845             


Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Leu Pro Gln Ser 
    850                 855                 860                 


Leu Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Asn Ala Arg 
865                 870                 875                 880 


Ile Asn Arg Glu Lys Asn Asp Thr Phe Ala Ser Glu Lys Phe Gly Lys 
                885                 890                 895     


Lys Met Gly Ala Lys Trp Lys Glu Met His Arg Met Gly Leu Met Thr 
            900                 905                 910         


Lys Arg Lys Leu Asp His Leu Leu Met Arg Pro Asp Glu Ile Ser Lys 
        915                 920                 925             


Tyr Ala Thr Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val 
    930                 935                 940                 


Ile Lys Leu Val Glu Glu Leu Ile Asn Ala Glu Tyr Pro Ser Thr Ala 
945                 950                 955                 960 


Ile Val Ser Val Lys Ala Asn Leu Thr His Gln Phe Arg Gln Thr Phe 
                965                 970                 975     


Asp Phe Pro Lys Ile Arg Glu Val Asn Asp Tyr His His Ala Phe Asp 
            980                 985                 990         


Ala Ala Leu Thr Ala Phe Ile Gly  Thr Tyr Leu Leu Lys  Arg Tyr Pro 
        995                 1000                 1005             


Lys Leu  Glu Arg Phe Phe Val  Tyr Gly Lys Phe Ala  Lys Gln Pro 
    1010                 1015                 1020             


Val Asn  Leu Thr Arg Phe Asn  Val Ile Arg Lys Leu  Ala Lys Thr 
    1025                 1030                 1035             


Asp Thr  Pro Ile Thr Asp Ile  Asp Thr Gly Glu Val  Leu Trp Asp 
    1040                 1045                 1050             


Lys Leu  Thr Asp Ile Lys Tyr  Phe Glu Thr Leu Tyr  Asp Tyr Lys 
    1055                 1060                 1065             


Arg Leu  Leu Val Thr Arg Glu  Val Ser Glu Asn Tyr  Gly Ala Met 
    1070                 1075                 1080             


Phe Asp  Gln Thr Leu Leu Lys  Ala Ser Asp Asn Gly  Lys Arg Leu 
    1085                 1090                 1095             


Ile Arg  Lys Lys Gln Ser Phe  Asp Thr Ala Ile Tyr  Gly Gly Tyr 
    1100                 1105                 1110             


Thr Gly  Lys Thr Leu Ala Tyr  Met Ala Ile Val Lys  Val Ser Thr 
    1115                 1120                 1125             


Lys Lys  Asp Val Gln Tyr Gln  Val Val Gly Val Pro  Thr Thr Met 
    1130                 1135                 1140             


Val Thr  Gln Ile Glu Gln Leu  Glu Gln Gln Gly Met  Thr Asn Lys 
    1145                 1150                 1155             


Gln Ala  Val Thr Lys Val Ile  Glu Pro Leu Ile Ala  Ala Asn Asn 
    1160                 1165                 1170             


Lys Lys  Met Leu Thr Tyr Glu  Val Leu Leu Pro Lys  Val Arg Phe 
    1175                 1180                 1185             


Glu Gln  Val Val Arg Asp Arg  Ile Lys Gly Gln Thr  His Arg Phe 
    1190                 1195                 1200             


Ala Leu  Gly Thr Asp Thr Tyr  Tyr His Asn Ile Gln  Glu Leu Tyr 
    1205                 1210                 1215             


Leu Pro  Leu Thr Leu Gln Arg  Ala Phe Leu Gly Lys  Gln Asn Glu 
    1220                 1225                 1230             


Ser Glu  Val Gln Leu Asn Gln  Asn Leu Leu Thr Val  Phe Asp Ala 
    1235                 1240                 1245             


Thr Leu  Ala Gln Val Asn Arg  Tyr Phe Pro Leu Tyr  Glu Met Asn 
    1250                 1255                 1260             


Lys Phe  Lys Lys Ile Leu Asn  Ser Ala Arg Asp Gln  Ile Glu Leu 
    1265                 1270                 1275             


Leu Glu  Ala Lys Asn Gln Phe  Val Lys Asn Lys Leu  Val Leu Gly 
    1280                 1285                 1290             


Lys Gln  Gly Ile Leu Thr Asn  Met Phe Ile Gly Leu  His Ala Asn 
    1295                 1300                 1305             


Ala Ser  Phe Gly Asp Leu Lys  Ser Leu Gly Val Lys  Thr Ser Phe 
    1310                 1315                 1320             


Gly Gln  Leu Gln Val Pro Gly  Gly Ile Lys Leu Thr  Gly Asp Ala 
    1325                 1330                 1335             


Glu Ile  Ile Tyr Gln Ser Pro  Thr Gly Leu Phe Glu  Arg Lys Ile 
    1340                 1345                 1350             


Ala Leu  Lys Asp Leu 
    1355             


<210>  945
<211>  1358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  945

Met Lys Gln Gly Lys Arg Gly Tyr Tyr Leu Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asp Ser Ile Gly Tyr Ala Val Thr Asp Glu Gln Tyr His Leu Leu Arg 
            20                  25                  30          


Phe His Gly Asn Asp Ala Trp Gly Ser His Val Phe Asp Ser Ala Ser 
        35                  40                  45              


Leu Cys Asp Glu Arg Arg Gly Phe Arg Ser Ala Arg Arg Arg Leu Asp 
    50                  55                  60                  


Arg Arg Gln Gln Arg Val Lys Leu Leu Gln Glu Ile Phe Ala Lys Glu 
65                  70                  75                  80  


Ile Ala Asn Ile Asp Pro Arg Phe Phe Ile Arg Leu Ser Glu Ser Tyr 
                85                  90                  95      


Arg Trp Arg Asp Asp Thr Asp Asp Arg Tyr Ile Tyr Phe Asn Glu Glu 
            100                 105                 110         


Glu Tyr Thr Asp Ile Gln Tyr Met Arg Asp Tyr Pro Thr Ile His His 
        115                 120                 125             


Leu Ile Cys Glu Leu Met Glu Asn Glu Glu Glu His Asp Ile Arg Leu 
    130                 135                 140                 


Val Tyr Leu Ala Cys Ala Trp Leu Val Ala His Arg Gly His Phe Leu 
145                 150                 155                 160 


Ser Gln Leu Ser Val Asp Asn Ile Asp Ser Leu Ile Asp Ile Glu Ser 
                165                 170                 175     


Val Tyr Gln Lys Phe Leu His Phe Phe Ile Asp Asn Gly Cys Glu Asn 
            180                 185                 190         


Pro Trp Ile Gln Asp Ala Glu Leu Val Gly Gly Ile Leu Lys Glu His 
        195                 200                 205             


Ile Gly Val Thr Glu Lys His Asn Arg Leu Gly Glu Ile Met Leu Gly 
    210                 215                 220                 


Asn Arg Lys Pro Ser Lys Asp Val Arg Asp Thr Phe Leu Tyr Ser Glu 
225                 230                 235                 240 


Tyr Cys Ile Ile Arg Leu Leu Ala Gly Gly Thr Cys Lys Leu Arg Asp 
                245                 250                 255     


Leu Tyr Gly Lys Glu Glu Tyr Glu Asp Phe Gly Ser Val Ser Leu Glu 
            260                 265                 270         


Met Asp Asp Asp Lys Leu Ala Glu Ile Met Ile Asn Ile Gly Glu Asp 
        275                 280                 285             


Tyr Glu Leu Ile Ala Val Leu Arg Ser Leu His Asp Trp Ser Val Leu 
    290                 295                 300                 


Ala Asp Ile Leu Asp Gly Gln Asn Gly Leu Thr Asn Ile Ser Thr Ala 
305                 310                 315                 320 


Lys Val Ala Ile Tyr Glu Gln His Lys Lys Asp Leu Ala Thr Leu Lys 
                325                 330                 335     


Tyr Phe Ile Arg Lys Tyr Cys Pro Glu Lys Tyr Asn Ala Ile Phe Lys 
            340                 345                 350         


Asp Ala Arg Glu Asp Asn Tyr Val Ala Tyr Ser Tyr His Tyr Asn Asn 
        355                 360                 365             


Lys Leu Asp Gly Gln Ile Lys Lys Lys Ala Asp Thr Glu Thr Phe Ser 
    370                 375                 380                 


Lys Phe Leu Ile Lys Ile Ile His Ser Ile Lys Pro Asp Lys Glu Asp 
385                 390                 395                 400 


Glu Ile Ile Tyr Gln Asp Met Asp Asn Arg Leu Gly Leu Arg Gln Phe 
                405                 410                 415     


Leu Pro Lys Gln Arg Asn Thr Asp Asn Arg Val Ile Pro His Gln Leu 
            420                 425                 430         


Tyr Glu Tyr Glu Leu Lys Lys Ile Leu Glu Asn Ala Ala Lys Tyr Leu 
        435                 440                 445             


Ser Phe Leu Asn Gln Lys Glu Asp Gly Ile Ser Asn Thr Glu Lys Ile 
    450                 455                 460                 


Ile Ser Ile Phe Lys Phe Lys Ile Pro Tyr Tyr Val Gly Pro Leu Asn 
465                 470                 475                 480 


Lys His Ser Glu Phe Ala Trp Ile Asp Arg Lys Ser Gly Lys Ile Leu 
                485                 490                 495     


Pro Trp Asn Tyr Glu Asn Met Ile Asp Asp Asp Ala Ser Glu Glu Ala 
            500                 505                 510         


Phe Ile Lys Lys Met Thr Asn Gln Cys Thr Tyr Leu Pro Gly Glu Ser 
        515                 520                 525             


Val Leu Pro Lys Asp Ser Leu Cys Tyr Gln Lys Phe Met Val Leu Asn 
    530                 535                 540                 


Glu Ile Asn Asn Ile Lys Val Asp Gly Arg Lys Ile Ser Val Gly Ala 
545                 550                 555                 560 


Lys Gln Gly Ile Tyr Ser Asp Leu Phe Leu Lys Tyr Lys Lys Val Lys 
                565                 570                 575     


Arg Ser Gln Ile Glu Glu Tyr Leu Ile Ser Asn Gly Gln Leu Glu Lys 
            580                 585                 590         


Asn Arg Arg Glu Thr Leu Ser Gly Ile Asp Ile Gln Ile Lys Ser Ser 
        595                 600                 605             


Leu Ser Ser His Ile Ala Phe Arg Arg Leu Leu Glu Gln Lys Ile Leu 
    610                 615                 620                 


Asn Glu Ala Asp Val Glu Arg Ile Ile Glu Arg Ala Ser Tyr Ala Glu 
625                 630                 635                 640 


Asp Lys Gly Arg Val Ala Lys Trp Leu Arg Ala Lys Tyr Pro His Leu 
                645                 650                 655     


Thr Glu Ala Asp Ile Lys Tyr Ile Cys Lys Val Lys Ile Lys Asp Phe 
            660                 665                 670         


Gly Arg Leu Ser Arg Thr Phe Leu Thr Gly Ile Glu Gly Ala Cys Lys 
        675                 680                 685             


Glu Thr Gly Glu Val Ala Thr Ile Ile Ser Met Met Trp Glu Ser Asn 
    690                 695                 700                 


Asp Asn Leu Met Glu Leu Leu Ser Glu Arg Tyr Thr Phe Ala Asp Thr 
705                 710                 715                 720 


Ile Leu Glu Phe Lys Ser Asp Tyr Tyr Thr Glu Arg Lys Gln Thr Leu 
                725                 730                 735     


Ser Glu Arg Leu Asp Glu Met Tyr Val Ser Asn Ala Val Arg Arg Pro 
            740                 745                 750         


Ile Tyr Arg Thr Leu Asp Ile Val Lys Asp Leu Glu Lys Ala Phe Gly 
        755                 760                 765             


Lys Pro Glu Lys Ile Phe Ile Glu Met Thr Arg Gly Ala Met Pro Glu 
    770                 775                 780                 


Leu Lys Gly Lys Arg Thr Lys Ser Arg Gln Gln Gln Leu Leu Glu Tyr 
785                 790                 795                 800 


Tyr Asp Lys Cys Lys Glu Glu Asp Val Arg Asp Leu Lys Gln Gln Leu 
                805                 810                 815     


Glu Ala Leu Gly Glu Tyr Val Asp Asn Lys Leu Gln Ser Asp Arg Leu 
            820                 825                 830         


Phe Leu Tyr Phe Met Gln Phe Gly Lys Cys Ala Tyr Ser Gly Met Pro 
        835                 840                 845             


Ile Ser Leu Glu Arg Leu Met Ala Gly Ser Lys Glu Tyr Asp Ile Asp 
    850                 855                 860                 


His Ile Tyr Pro Gln Ala Tyr Val Lys Asp Asp Ser Ile Ile Asn Asn 
865                 870                 875                 880 


Lys Val Leu Val Leu Ser Val Ala Asn Gly Glu Lys Lys Asp Val Tyr 
                885                 890                 895     


Pro Ile Lys Ser Glu Ile Arg Asn Lys Met Gln Lys Thr Trp Ser Phe 
            900                 905                 910         


Trp His His Val Gly Thr Ile Ser Asp Glu Lys Tyr Lys Arg Leu Ile 
        915                 920                 925             


Arg Ser Thr Pro Phe Thr Glu Asp Glu Lys Tyr Gly Phe Ile Asn Arg 
    930                 935                 940                 


Gln Leu Thr Glu Thr Ser Gln Ser Thr Lys Val Val Ala Glu Leu Leu 
945                 950                 955                 960 


Lys Glu Arg Tyr Pro Glu Ala Glu Ile Val Tyr Ser Lys Ala Gly Leu 
                965                 970                 975     


Ile Ser Asp Phe Arg His Glu Phe Asp Leu Tyr Lys Ser Arg Ser Tyr 
            980                 985                 990         


Asn Asp Leu His His Ala Val Asp  Ala Tyr Leu Asn Ile  Val Val Gly 
        995                 1000                 1005             


Asn Val  Tyr His Met Arg Phe  Ser Arg Gln Trp Phe  Asn Val Asn 
    1010                 1015                 1020             


Ser Ser  Tyr Ser Ile Lys Thr  Lys Thr Leu Phe Thr  His Thr Val 
    1025                 1030                 1035             


Asn Cys  Asn Gly Lys Glu Val  Trp Asn Lys Asp Met  Leu Pro Gly 
    1040                 1045                 1050             


Val Leu  Lys Thr Ala Lys Lys  Asn Thr Ala His Phe  Thr Lys His 
    1055                 1060                 1065             


Ala Thr  Phe Lys Thr Gly Gly  Leu Phe Asp Gln Asn  Pro Val Lys 
    1070                 1075                 1080             


Lys Ala  Pro Gly Leu Thr Pro  Leu Lys Ala Gly Leu  Pro Thr Glu 
    1085                 1090                 1095             


Lys Tyr  Gly Gly Tyr Asn Lys  Ala Gly Val Met Phe  Phe Ile Pro 
    1100                 1105                 1110             


Val Arg  Tyr Lys Ser Gly Lys  Lys Thr Val Leu Met  Val Leu Ser 
    1115                 1120                 1125             


Val Glu  Leu Leu Tyr Gly Asn  Arg Phe Leu Glu Asp  Glu Ile Phe 
    1130                 1135                 1140             


Ala Lys  Glu Tyr Ala Lys Asp  Arg Leu Gln Arg Met  Ile Gly Lys 
    1145                 1150                 1155             


Ser Val  Asp Glu Ile Asp Phe  Pro Ile Gly Met Arg  Pro Trp Lys 
    1160                 1165                 1170             


Val Asn  Thr Ile Leu Ser Leu  Asp Gly Phe Arg Ile  Cys Ile Thr 
    1175                 1180                 1185             


Gly Asn  Ala Ser Gly Gly Lys  Cys Leu Ile Ala Gln  Pro Ile Met 
    1190                 1195                 1200             


Gln Phe  Ser Ser Asp Glu Tyr  Trp Lys Tyr Tyr Leu  Lys Lys Val 
    1205                 1210                 1215             


Glu Lys  Phe Val Glu Lys Val  Lys Asn Asn Ser Ser  Tyr Val Tyr 
    1220                 1225                 1230             


Asp Val  Asp Tyr Asp Ala Val  Gln His Glu Asp Asn  Leu Lys Leu 
    1235                 1240                 1245             


Tyr Asp  Leu Tyr Leu Asp Lys  Leu Gln Asn Ser Ile  Tyr Arg Lys 
    1250                 1255                 1260             


Arg Ile  Asn Ala Pro Ile Gln  Thr Leu Ile Glu Gly  Arg Glu Lys 
    1265                 1270                 1275             


Phe Ile  Asp Cys Ser Val Ile  Glu Gln Cys Gln Val  Leu Leu Asn 
    1280                 1285                 1290             


Ile His  Gln Val Phe Gly Arg  Met Thr Ser Gly Cys  Asp Leu Thr 
    1295                 1300                 1305             


Leu Ile  Gly Gly Lys Thr Arg  Ser Ala Ser Thr Val  Asn Phe Ser 
    1310                 1315                 1320             


Ser Ile  Ile Leu Asn Trp Lys  Lys Asn Tyr Thr Asp  Val Arg Ile 
    1325                 1330                 1335             


Ile Asp  Gln Ser Ala Ser Gly  Leu Trp Glu Val Val  Ser Glu Asn 
    1340                 1345                 1350             


Ile Leu  Glu Tyr Leu 
    1355             


<210>  946
<211>  1358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  946

Met Gly Lys Met Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Tyr Ala Val Thr Asp Pro Ser Tyr His Leu Leu Lys Phe Lys Gly 
            20                  25                  30          


Glu Pro Met Trp Gly Ala His Val Phe Ala Ala Gly Asn Gln Ser Ala 
        35                  40                  45              


Glu Arg Arg Ser Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Gln 
    50                  55                  60                  


Gln Arg Val Lys Leu Val Gln Glu Ile Phe Ala Pro Val Ile Ser Pro 
65                  70                  75                  80  


Ile Asp Pro Arg Phe Phe Ile Arg Leu His Glu Ser Ala Leu Trp Arg 
                85                  90                  95      


Asp Asp Val Ala Glu Thr Asp Lys His Ile Phe Phe Asn Asp Pro Thr 
            100                 105                 110         


Tyr Thr Asp Lys Glu Tyr Tyr Ser Asp Tyr Pro Thr Ile His His Leu 
        115                 120                 125             


Ile Val Asp Leu Met Glu Ser Ser Glu Lys His Asp Pro Arg Leu Val 
    130                 135                 140                 


Tyr Leu Ala Val Ala Trp Leu Val Ala His Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Glu Val Asp Lys Asp Asn Ile Gly Asp Val Leu Ser Phe Asp Ala Phe 
                165                 170                 175     


Tyr Pro Glu Phe Leu Ala Phe Leu Ser Asp Asn Gly Val Ser Pro Trp 
            180                 185                 190         


Val Cys Glu Ser Lys Ala Leu Gln Ala Thr Leu Leu Ser Arg Asn Ser 
        195                 200                 205             


Val Asn Asp Lys Tyr Lys Ala Leu Lys Ser Leu Ile Phe Gly Ser Gln 
    210                 215                 220                 


Lys Pro Glu Asp Asn Phe Asp Ala Asn Ile Ser Glu Asp Gly Leu Ile 
225                 230                 235                 240 


Gln Leu Leu Ala Gly Lys Lys Val Lys Val Asn Lys Leu Phe Pro Gln 
                245                 250                 255     


Glu Ser Asn Asp Ala Ser Phe Thr Leu Asn Asp Lys Glu Asp Ala Ile 
            260                 265                 270         


Glu Glu Ile Leu Gly Thr Leu Thr Pro Asp Glu Cys Glu Trp Ile Ala 
        275                 280                 285             


His Ile Arg Arg Leu Phe Asp Trp Ala Ile Met Lys His Ala Leu Lys 
    290                 295                 300                 


Asp Gly Arg Thr Ile Ser Glu Ser Lys Val Lys Leu Tyr Glu Gln His 
305                 310                 315                 320 


His His Asp Leu Thr Gln Leu Lys Tyr Phe Val Lys Thr Tyr Leu Ala 
                325                 330                 335     


Lys Glu Tyr Asp Asp Ile Phe Arg Asn Val Asp Ser Glu Thr Thr Lys 
            340                 345                 350         


Asn Tyr Val Ala Tyr Ser Tyr His Val Lys Glu Val Lys Gly Thr Leu 
        355                 360                 365             


Pro Lys Asn Lys Ala Thr Gln Glu Glu Phe Cys Lys Tyr Val Leu Gly 
    370                 375                 380                 


Lys Val Lys Asn Ile Glu Cys Ser Glu Ala Asp Lys Val Asp Phe Asp 
385                 390                 395                 400 


Glu Met Ile Gln Arg Leu Thr Asp Asn Ser Phe Met Pro Lys Gln Val 
                405                 410                 415     


Ser Gly Glu Asn Arg Val Ile Pro Tyr Gln Leu Tyr Tyr Tyr Glu Leu 
            420                 425                 430         


Lys Thr Ile Leu Asn Lys Ala Ala Ser Tyr Leu Pro Phe Leu Thr Gln 
        435                 440                 445             


Cys Gly Lys Asp Ala Ile Ser Asn Gln Asp Lys Leu Leu Ser Ile Met 
    450                 455                 460                 


Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Arg Lys Asp Asn Ser 
465                 470                 475                 480 


Glu His Ala Trp Leu Glu Arg Lys Ala Gly Lys Ile Tyr Pro Trp Asn 
                485                 490                 495     


Phe Asn Asp Lys Val Asp Leu Asp Lys Ser Glu Glu Ala Phe Ile Arg 
            500                 505                 510         


Arg Met Thr Asn Thr Cys Thr Tyr Tyr Pro Gly Glu Asp Val Leu Pro 
        515                 520                 525             


Leu Asp Ser Leu Ile Tyr Glu Lys Phe Met Ile Leu Asn Glu Ile Asn 
    530                 535                 540                 


Asn Ile Arg Ile Asp Gly Tyr Pro Ile Ser Val Asp Val Lys Gln Gln 
545                 550                 555                 560 


Val Phe Gly Leu Phe Glu Lys Lys Arg Arg Val Thr Val Lys Asp Ile 
                565                 570                 575     


Gln Asn Leu Leu Leu Ser Leu Gly Ala Leu Asp Lys His Gly Lys Leu 
            580                 585                 590         


Thr Gly Ile Asp Thr Thr Ile His Ser Asn Tyr Asn Thr Tyr His His 
        595                 600                 605             


Phe Lys Ser Leu Met Glu Arg Gly Val Leu Thr Arg Asp Asp Val Glu 
    610                 615                 620                 


Arg Ile Val Glu Arg Met Thr Tyr Ser Asp Asp Thr Lys Arg Val Arg 
625                 630                 635                 640 


Leu Trp Leu Asn Asn Asn Tyr Gly Thr Leu Thr Ala Asp Asp Val Lys 
                645                 650                 655     


His Ile Ser Arg Leu Arg Lys His Asp Phe Gly Arg Leu Ser Lys Met 
            660                 665                 670         


Phe Leu Thr Gly Leu Lys Gly Val His Lys Glu Thr Gly Glu Arg Ala 
        675                 680                 685             


Ser Ile Leu Asp Phe Met Trp Asn Thr Asn Asp Asn Leu Met Gln Leu 
    690                 695                 700                 


Leu Ser Glu Cys Tyr Thr Phe Ser Asp Glu Ile Thr Lys Leu Gln Glu 
705                 710                 715                 720 


Ala Tyr Tyr Ala Lys Ala Gln Leu Ser Leu Asn Asp Phe Leu Asp Ser 
                725                 730                 735     


Met Tyr Ile Ser Asn Ala Val Lys Arg Pro Ile Tyr Arg Thr Leu Ala 
            740                 745                 750         


Val Val Asn Asp Ile Arg Lys Ala Cys Gly Thr Ala Pro Lys Arg Ile 
        755                 760                 765             


Phe Ile Glu Met Ala Arg Asp Gly Glu Ser Lys Lys Lys Arg Ser Val 
    770                 775                 780                 


Thr Arg Arg Glu Gln Ile Lys Asn Leu Tyr Arg Ser Ile Arg Lys Asp 
785                 790                 795                 800 


Phe Gln Gln Glu Val Asp Phe Leu Glu Lys Ile Leu Glu Asn Lys Ser 
                805                 810                 815     


Asp Gly Gln Leu Gln Ser Asp Ala Leu Tyr Leu Tyr Phe Ala Gln Leu 
            820                 825                 830         


Gly Arg Asp Met Tyr Thr Gly Asp Pro Ile Lys Leu Glu His Ile Lys 
        835                 840                 845             


Asp Gln Ser Phe Tyr Asn Ile Asp His Ile Tyr Pro Gln Ser Met Val 
    850                 855                 860                 


Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Val Gln Ser Glu Ile Asn 
865                 870                 875                 880 


Gly Glu Lys Ser Ser Arg Tyr Pro Leu Asp Ala Ala Ile Arg Asn Lys 
                885                 890                 895     


Met Lys Pro Leu Trp Asp Ala Tyr Tyr Asn His Gly Leu Ile Ser Leu 
            900                 905                 910         


Lys Lys Tyr Gln Arg Leu Thr Arg Ser Thr Pro Phe Thr Asp Asp Glu 
        915                 920                 925             


Lys Trp Asp Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
    930                 935                 940                 


Lys Ala Leu Ala Ile Leu Leu Lys Arg Lys Phe Pro Asp Thr Glu Ile 
945                 950                 955                 960 


Val Tyr Ser Lys Ala Gly Leu Ser Ser Asp Phe Arg His Glu Phe Gly 
                965                 970                 975     


Leu Val Lys Ser Arg Asn Ile Asn Asp Leu His His Ala Lys Asp Ala 
            980                 985                 990         


Phe Leu Ala Ile Val Thr Gly Asn  Val Tyr His Glu Arg  Phe Asn Arg 
        995                 1000                 1005             


Arg Trp  Phe Met Val Asn Gln  Pro Tyr Ser Val Lys  Thr Lys Thr 
    1010                 1015                 1020             


Leu Phe  Thr His Ser Ile Lys  Asn Gly Asn Phe Val  Ala Trp Asn 
    1025                 1030                 1035             


Gly Glu  Glu Asp Leu Gly Arg  Ile Val Lys Met Leu  Lys Gln Asn 
    1040                 1045                 1050             


Lys Asn  Thr Ile His Phe Thr  Arg Phe Ser Phe Asp  Arg Lys Glu 
    1055                 1060                 1065             


Gly Leu  Phe Asp Ile Gln Pro  Leu Lys Ala Ser Thr  Gly Leu Val 
    1070                 1075                 1080             


Pro Arg  Lys Ala Gly Leu Asp  Val Val Lys Tyr Gly  Gly Tyr Asp 
    1085                 1090                 1095             


Lys Ser  Thr Ala Ala Tyr Tyr  Leu Leu Val Arg Phe  Thr Leu Glu 
    1100                 1105                 1110             


Asp Lys  Lys Thr Gln His Lys  Leu Met Met Ile Pro  Val Glu Gly 
    1115                 1120                 1125             


Leu Tyr  Lys Ala Arg Ile Asp  His Asp Lys Glu Phe  Leu Thr Asp 
    1130                 1135                 1140             


Tyr Ala  Gln Thr Thr Ile Ser  Glu Ile Leu Gln Lys  Asp Lys Gln 
    1145                 1150                 1155             


Lys Val  Ile Asn Ile Met Phe  Pro Met Gly Thr Arg  His Ile Lys 
    1160                 1165                 1170             


Leu Asn  Ser Met Ile Ser Ile  Asp Gly Phe Tyr Leu  Ser Ile Gly 
    1175                 1180                 1185             


Gly Lys  Ser Ser Lys Gly Lys  Ser Val Leu Cys His  Ala Met Val 
    1190                 1195                 1200             


Pro Leu  Ile Val Pro His Lys  Ile Glu Cys Tyr Ile  Lys Ala Met 
    1205                 1210                 1215             


Glu Ser  Phe Ala Arg Lys Phe  Lys Glu Asn Asn Lys  Leu Arg Ile 
    1220                 1225                 1230             


Val Glu  Lys Phe Asp Lys Ile  Thr Val Glu Asp Asn  Leu Asn Leu 
    1235                 1240                 1245             


Tyr Glu  Leu Phe Leu Gln Lys  Leu Gln His Asn Pro  Tyr Asn Lys 
    1250                 1255                 1260             


Phe Phe  Ser Thr Gln Phe Asp  Val Leu Thr Asn Gly  Arg Ser Thr 
    1265                 1270                 1275             


Phe Thr  Lys Leu Ser Pro Glu  Glu Gln Val Gln Thr  Leu Leu Asn 
    1280                 1285                 1290             


Ile Leu  Ser Ile Phe Lys Thr  Cys Arg Ser Ser Gly  Cys Asp Leu 
    1295                 1300                 1305             


Lys Ser  Ile Asn Gly Ser Ala  Gln Ala Ala Arg Ile  Met Ile Ser 
    1310                 1315                 1320             


Ala Asp  Leu Thr Gly Leu Ser  Lys Lys Tyr Ser Asp  Ile Arg Leu 
    1325                 1330                 1335             


Val Glu  Gln Ser Ala Ser Gly  Leu Phe Val Ser Lys  Ser Gln Asn 
    1340                 1345                 1350             


Leu Leu  Glu Tyr Leu 
    1355             


<210>  947
<211>  1360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  947

Met Thr Lys Leu Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg 
            20                  25                  30          


Leu Lys Gly Lys Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys 
        35                  40                  45              


Ala Ala Ala Asp Arg Arg Ala Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Asn Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His 
65                  70                  75                  80  


Ile Thr Pro Thr Asp Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Ala Arg Tyr Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Gln Gln Tyr Pro Thr Met Tyr 
        115                 120                 125             


His Leu Arg Thr Arg Leu Leu Thr Asp Pro Ser Lys Ala Asp Val Arg 
    130                 135                 140                 


Glu Ile Tyr Phe Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Ser Gly Tyr Ala Lys Asp Phe Asn Thr Asn Lys Val Ala Leu 
                165                 170                 175     


Asn Glu Ile Phe Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Asp Ile Thr Phe Asp Glu Asn Lys Val Asn Glu Phe Lys Thr 
        195                 200                 205             


Val Leu Leu Asp Glu Lys Ala Thr Pro Ser Asp Thr Gln Arg Ala Leu 
    210                 215                 220                 


Val Asn Leu Leu Leu Ala Glu Asp Gly Asp Lys Asp Ile Leu Lys Gln 
225                 230                 235                 240 


Gln Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Val Val Gly Leu Lys 
                245                 250                 255     


Thr Lys Leu Asn Val Ala Leu Gly Thr Glu Val Asp Ser Ser Glu Ala 
            260                 265                 270         


Thr Ala Trp Asn Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp Ala Gly 
        275                 280                 285             


Ile Glu Ser Ala Met Thr Asp Glu Gly Thr Glu Ile Leu Asp Gln Ile 
    290                 295                 300                 


Arg Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Glu Gln His His Glu 
                325                 330                 335     


Asp Leu Glu Leu Phe Lys Asp Tyr Leu Lys Gln Leu Glu Asp Asp Gly 
            340                 345                 350         


Met Ala Lys Ala Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Val Arg Glu Asp Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Asn Ala Lys Ser Pro Glu Leu Leu Glu Arg 
385                 390                 395                 400 


Leu Val Gln Pro Asp Phe Met Leu Lys Gln Arg Asn Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Gln Ile Ile Lys 
            420                 425                 430         


Asn Gln Ser Val Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Gln Ser Met Pro Tyr Gln Phe Asp Glu Leu Leu Asn Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Ala Lys Glu Gln Lys 
465                 470                 475                 480 


Ala Ala Gln Gly Gly Val Phe Ala Trp Met Val Arg Lys Asp Pro Asp 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Ser Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asp Asn Lys Leu 
545                 550                 555                 560 


Ser Phe Asp Gln Lys Gln Gln Val Leu Gly Glu Leu Phe Glu Arg His 
                565                 570                 575     


Asn Thr Val Thr Ile Lys Gln Phe Val Asp Asn Leu Leu Ala His Gly 
            580                 585                 590         


Asp Tyr Asp Arg Ile Pro Glu Val Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr Asn Gln Leu Lys Gly Ile Leu His 
    610                 615                 620                 


Glu Ala Ile Asp Asp Ser Asp Lys Gln Thr Asp Ile Glu Lys Ile Ile 
625                 630                 635                 640 


Ala Trp Ser Thr Ile Phe Glu Asp Lys Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Asn Glu Ile Ser Trp Leu Asn Ala Gly Met Ile Asn Ser Leu Ala Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser His Lys Leu Leu Asn Glu 
        675                 680                 685             


Leu Thr Leu Ser Thr Gly Arg Thr Val Phe Glu Glu Leu Leu Ile Ser 
    690                 695                 700                 


Ser His Asn Leu Met Gln Ile Leu Thr Asp Glu Val Leu Lys Gln Lys 
705                 710                 715                 720 


Ile Ala Glu Leu Asn Ala His Glu Leu Lys Ala Ser Gly Ile Tyr Ala 
                725                 730                 735     


Ala Ile Asp Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Ser Val Ile Asp Asp Ile Thr Asn Ala Ala Gly Gln Ala 
        755                 760                 765             


Pro Asn Trp Leu Tyr Ile Glu Thr Ala Asp Gly Asp Gly Val Pro Gly 
    770                 775                 780                 


Lys Arg Thr Asn Ser Arg Gln Arg Lys Ile Gln Glu Ile Tyr Val Asn 
785                 790                 795                 800 


Ala Ala Gln Glu Leu Ile Thr Gly Ser Val Arg Gly Glu Leu Glu Asp 
                805                 810                 815     


Lys Ile Ala Ser Arg Ala Asp Phe Thr Asp Arg Leu Val Leu Tyr Phe 
            820                 825                 830         


Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Glu Pro Leu Asn Ile Asp 
        835                 840                 845             


Asn Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu Ile 
    850                 855                 860                 


Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val His Pro Leu Val Asn 
865                 870                 875                 880 


Arg Glu Lys Asp Thr Ile Phe Ala Ser Thr Arg Phe Ala Lys Gln Met 
                885                 890                 895     


Asn Gly Met Trp Arg Gln Trp His Ala Ala Gly Leu Ile Ser Ser Arg 
            900                 905                 910         


Lys Leu Arg Asn Leu Gln Met Arg Pro Asp Glu Ile Asp Lys Tyr Ala 
        915                 920                 925             


Ser Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys 
    930                 935                 940                 


Leu Thr Glu Gln Ile Val Ala Asp Gln Tyr Pro Asp Ala Lys Ile Ile 
945                 950                 955                 960 


Ala Val Lys Ala Gly Leu Ser Ser Gln Leu Arg Lys Glu Leu Asp Phe 
                965                 970                 975     


Pro Lys Asn Arg Glu Val Asn His Tyr His His Ala Phe Asp Ala Phe 
            980                 985                 990         


Leu Ala Ala Arg Ile Gly Thr Tyr  Leu Leu Lys Arg Tyr  Pro Asn Leu 
        995                 1000                 1005             


Glu Pro  Phe Phe Thr Tyr Gly  Lys Phe Lys Lys Thr  Glu Val Lys 
    1010                 1015                 1020             


Lys Leu  Lys Ser Phe Asn Phe  Ile Arg Asp Met Thr  His Ala Lys 
    1025                 1030                 1035             


Asp Lys  Ile Val Ala Lys Glu  Thr Gly Glu Ile Val  Trp Asp Asn 
    1040                 1045                 1050             


Ala Ser  Asp Ile Asn Glu Leu  Asp Arg Ile Tyr Asn  Phe Lys Arg 
    1055                 1060                 1065             


Met Leu  Ile Thr His Glu Val  Arg Phe Glu Thr Ala  Ser Leu Phe 
    1070                 1075                 1080             


Lys Gln  Thr Leu Tyr Ala Ala  Lys Asn Ser Lys Asn  Arg Gly Gly 
    1085                 1090                 1095             


Ser Arg  Gln Leu Ile Pro Lys  Lys Lys Gly Tyr Leu  Val Asp Ile 
    1100                 1105                 1110             


Tyr Gly  Gly Tyr Thr Gln Glu  Thr Gly Ser Tyr Leu  Ser Val Val 
    1115                 1120                 1125             


Arg Leu  Thr Lys Lys Ala Met  Tyr Ala Val Val Lys  Val Ser Thr 
    1130                 1135                 1140             


Arg Asp  Ala Ala Lys Leu Ala  Val Ala Lys Ser Ile  Ser Glu Gln 
    1145                 1150                 1155             


Lys Glu  Asn Glu Thr Leu Lys  Lys Ile Ile Asp Gly  Lys Leu Ser 
    1160                 1165                 1170             


Lys Thr  Ser Lys Lys Gly Lys  Thr Thr His Gln Leu  Phe Glu Ile 
    1175                 1180                 1185             


Val Leu  Pro Arg Val Gly Gln  Lys Thr Leu Phe Lys  Asn Ser Lys 
    1190                 1195                 1200             


Tyr Asn  Gln Phe Leu Val Asn  Ser Asp Thr Tyr Met  His Asn Tyr 
    1205                 1210                 1215             


Gln Glu  Leu Trp Met Pro Arg  Glu Tyr Gln Arg Met  Trp Lys Asp 
    1220                 1225                 1230             


Ile Leu  Leu Ser Asn His Gly  Asp Ala Gln Leu Glu  Gly Gln Leu 
    1235                 1240                 1245             


Asp Gln  Ile Phe Lys Phe Ile  Val Ser Gln Val Asn  Ser Tyr Phe 
    1250                 1255                 1260             


Asn Leu  Tyr Asp Ile Asn Gln  Phe Arg Lys Lys Ile  Ala Asp Ala 
    1265                 1270                 1275             


Thr Asp  Lys Phe Ala Lys Leu  Pro Thr His Asp Thr  Asp Asn Ala 
    1280                 1285                 1290             


Arg Gly  Lys Ile Ala Thr Ile  Gly Gln Leu Leu Ile  Gly Leu Gln 
    1295                 1300                 1305             


Ala Asn  Ala Met Arg Ser Asp  Leu Arg Asn Leu Asp  Ile Lys Thr 
    1310                 1315                 1320             


Pro Phe  Gly Leu Leu Gln Ile  Gly Thr Gly Ile Thr  Leu Asp Leu 
    1325                 1330                 1335             


Asp Thr  Ser Ile Ile Tyr Gln  Ser Pro Thr Gly Leu  Phe Glu Arg 
    1340                 1345                 1350             


Glu Val  Pro Leu Thr Asp Leu  
    1355                 1360 


<210>  948
<211>  1360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  948

Met Thr Lys Leu Asn Gln Pro Tyr Gly Ile Gly Leu Asp Ile Gly Ser 
1               5                   10                  15      


Ser Ser Ile Gly Phe Ala Val Val Asp Ala Asn Ser His Leu Leu Arg 
            20                  25                  30          


Leu Lys Gly Glu Thr Ala Ile Gly Ala Arg Leu Phe Arg Glu Gly Glu 
        35                  40                  45              


Ser Ala Ala Gly Arg Arg Ala Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


His Asn Arg Trp Arg Leu Arg Phe Leu Gln Asp Phe Phe Asp Pro His 
65                  70                  75                  80  


Ile Ser Ser Ile Asp Pro Asp Phe Phe Leu Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Glu Arg Phe Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Gln Phe Tyr Lys Gln Tyr Pro Thr Met Tyr 
        115                 120                 125             


His Leu Arg Leu His Leu Met Asn Asp Ala Ser Lys Ala Asp Pro Arg 
    130                 135                 140                 


Glu Ile Phe Phe Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Asp Ala Lys Asn Phe Asn Thr Asp Asp Val Asp Leu 
                165                 170                 175     


Lys His Ile Phe Pro Ala Leu Thr Glu Ala Tyr Ala Gln Ala Tyr Pro 
            180                 185                 190         


Asp Leu Thr Leu Ser Phe Glu Pro Ser Lys Ala Glu Lys Phe Lys Ser 
        195                 200                 205             


Ile Leu Leu Asp Glu Asp Ala Thr Ser Asn Asp Thr Gln Lys Ala Leu 
    210                 215                 220                 


Val Lys Leu Leu Leu Ser Asp Asp Gly Asp Lys Glu Val Leu Lys Gln 
225                 230                 235                 240 


Gln Lys Lys Val Leu Thr Glu Phe Ala Lys Ala Val Ala Gly Leu Lys 
                245                 250                 255     


Val Lys Phe Asn Val Ala Leu Gly Ile Glu Val Thr Asp Ala Asp Val 
            260                 265                 270         


Glu Lys Trp His Phe Gly Met Ala Gln Leu Glu Asp Lys Trp Ser Asp 
        275                 280                 285             


Ile Glu Pro Ser Leu Thr Asp Gln Gly Met Glu Ile Phe Glu Gln Ile 
    290                 295                 300                 


Gln Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Asp Gln His Cys Lys 
                325                 330                 335     


Asp Leu Lys Leu Phe Lys Glu Tyr Leu Lys Gln Leu Asn Asp His Asp 
            340                 345                 350         


Leu Ala Lys Arg Ile Arg Leu Leu Tyr Asp Arg Tyr Ile Asp Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Leu Arg Asp Asp Phe Val Lys Ala Leu Lys Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Thr Glu Leu Ala Glu Ser Leu Leu Glu Arg 
385                 390                 395                 400 


Met Glu Gln Asp Asn Phe Met Leu Lys Gln Arg Thr Lys Ala Asn Gly 
                405                 410                 415     


Ser Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Lys Ile Ile Ala 
            420                 425                 430         


Asn Gln Ala Glu Tyr Tyr Ser Trp Leu Ala Ala Glu Asn Pro Val Lys 
        435                 440                 445             


Glu His Arg Asp Ser Lys Pro Tyr Gln Leu Asp Glu Leu Leu Asn Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Met Ile Thr Lys Lys Gln Gln Glu 
465                 470                 475                 480 


Lys Ser Gly Gln Asn Val Phe Ala Trp Met Val Arg Lys Asp Pro Ser 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Ala Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Lys Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Gly Glu Ser Leu 
545                 550                 555                 560 


Ser Thr Asp Gln Lys Lys Arg Leu Ile Arg Glu Leu Phe Glu Arg His 
                565                 570                 575     


Ser Ser Val Thr Leu Lys Gln Phe Val Glu Asn Leu Val Ala His Gly 
            580                 585                 590         


Asp Val Ala Lys Arg Pro Asp Val Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr His Gln Leu Arg Gly Ile Leu His 
    610                 615                 620                 


Asp Ala Ile Asp Asp Arg Gly Lys Gln Ser Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Thr Trp Ser Thr Val Phe Glu Asp Ala Ser Ile Phe Lys Ser Lys Leu 
                645                 650                 655     


Gly Glu Ile Ser Trp Leu Asp Ser Lys Thr Arg Thr Thr Leu Ser Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Arg Lys Leu Leu Asp Gly 
        675                 680                 685             


Leu Lys Leu Gly Asn Gly His Thr Ile Ile Gln Glu Leu Met Leu Ser 
    690                 695                 700                 


Ser Arg Asn Leu Met Gln Ile Leu Thr Asp Glu Thr Leu Lys Lys Glu 
705                 710                 715                 720 


Met Ala Thr Leu Asn Gln Glu Arg Leu Lys Pro Asp Ser Ile Asn Glu 
                725                 730                 735     


Ala Ile Asp Ala Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Val Leu Lys Val Val Asp Asp Ile Lys His Ala Ala Asn Asp Gln 
        755                 760                 765             


Ala Pro Ser Trp Leu Tyr Ile Glu Thr Ala Asp Gly Gly Gly His Pro 
    770                 775                 780                 


Gly Lys Arg Thr Leu Ser Arg Gln Lys Gln Ile Gln Ala Ile Tyr Glu 
785                 790                 795                 800 


Asn Thr Ala Lys Glu Leu Ile Asp Ser Ala Val Arg Gly Glu Leu Lys 
                805                 810                 815     


Asp Lys Ile Ala Asp Lys Thr Asn Phe Ser Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly His Asp Ile Tyr Thr Gly Thr Ala Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val His Ala Lys Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Ala Glu Phe Ala Ser Thr Leu Phe Ala Ala Lys 
                885                 890                 895     


Met Ala Ser Thr Trp His Arg Trp Arg Asp Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Lys Asn Leu Leu Met Arg Pro Asp Glu Val Asp Lys Tyr 
        915                 920                 925             


Ala Lys Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Val Ala Ala Gln Tyr Pro Asp Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Gln Glu Leu Asp 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Tyr Pro Asn 
        995                 1000                 1005             


Leu Ala  Pro Phe Phe Thr Tyr  Gly Glu Phe Thr Lys  Thr Asn Leu 
    1010                 1015                 1020             


Lys Lys  Leu Gln Ser Phe Asn  Phe Ile Gly Ala Leu  Thr His Thr 
    1025                 1030                 1035             


Asp Gly  Lys Ile Thr Ser Lys  Asp Thr Gly Glu Val  Leu Trp Asp 
    1040                 1045                 1050             


Asn Gln  Arg Asp Ile Glu Glu  Leu Asp Gln Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val Tyr Phe Glu Thr  Ala Gly Leu 
    1070                 1075                 1080             


Phe Lys  Gln Thr Val Tyr Ala  Ala Lys Asp Ser Lys  Glu Arg Gly 
    1085                 1090                 1095             


Gly Ser  Arg Gln Leu Ile Pro  Lys Lys Lys Gly Tyr  Ser Thr Gly 
    1100                 1105                 1110             


Ile Tyr  Gly Gly Tyr Thr Gln  Glu Thr Gly Ser Tyr  Asn Ala Leu 
    1115                 1120                 1125             


Val Arg  Leu Thr Lys Ala Gly  Lys Val Val Tyr Arg  Val Phe Lys 
    1130                 1135                 1140             


Ile Ser  Thr Gln Asn Ala Ala  Glu Ile Ala Ala Ala  Arg Ser Leu 
    1145                 1150                 1155             


Ser Ala  Glu Glu Gly Asn Glu  Arg Leu Cys Gln Ile  Leu Glu Gln 
    1160                 1165                 1170             


Ser Leu  Leu Lys Thr Asn Lys  Asp Lys Lys Asn Thr  Val Asp Leu 
    1175                 1180                 1185             


Phe Asp  Val Val Ile Pro Arg  Leu Gly Lys Glu Ala  Leu Phe His 
    1190                 1195                 1200             


Asn Ser  Lys Tyr Gly Leu Phe  Leu Val Asn Ser Asp  Thr Tyr Tyr 
    1205                 1210                 1215             


His Asn  Tyr Gln Glu Leu Trp  Leu Ser Arg Glu Asn  Gln Lys Ile 
    1220                 1225                 1230             


Leu Lys  Lys Leu Phe Ser Thr  Lys Tyr Asp Gln Ser  Glu Met Asp 
    1235                 1240                 1245             


Arg Asp  Ala Leu His Leu Phe  Glu Thr Ile Ala Lys  Gln Val Glu 
    1250                 1255                 1260             


Gln Phe  Phe Lys Leu Tyr Asp  Ile Asn Gln Phe Arg  Thr Lys Ile 
    1265                 1270                 1275             


Ser Ser  Ala Phe Asp Ala Phe  Glu Lys Leu Pro Val  Glu Gly Glu 
    1280                 1285                 1290             


Glu Gly  Lys Val Glu Thr Leu  Lys Gln Leu Leu Ile  Gly Leu His 
    1295                 1300                 1305             


Ala Asn  Pro Val Arg Ser Asp  Leu Lys Asn Leu Gly  Ile Lys Thr 
    1310                 1315                 1320             


Pro Phe  Gly Phe Leu Gln Ala  Gly Lys Gly Ile His  Leu Asp Asp 
    1325                 1330                 1335             


Asp Thr  Glu Ile Ile Gln Gln  Ser Pro Ala Gly Leu  Phe Lys Arg 
    1340                 1345                 1350             


Arg Val  Arg Leu Ala Asp Leu  
    1355                 1360 


<210>  949
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  949

Met Thr Lys Leu Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg 
            20                  25                  30          


Leu Lys Gly Lys Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys 
        35                  40                  45              


Ala Ala Ala Asp Arg Arg Ala Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Asn Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His 
65                  70                  75                  80  


Ile Thr Pro Thr Gly Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Ala Arg Tyr Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Gln Gln Tyr Pro Thr Met Tyr 
        115                 120                 125             


His Leu Arg Asn Arg Leu Leu Thr Asp Pro Ser Lys Ala Asp Val Arg 
    130                 135                 140                 


Glu Ile Tyr Phe Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Ser Gly Tyr Ala Lys Asp Phe Asn Thr Asn Lys Val Ala Leu 
                165                 170                 175     


Asn Glu Ile Phe Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Asp Ile Thr Phe Asp Glu Asn Lys Val Asn Glu Phe Lys Thr 
        195                 200                 205             


Val Leu Leu Asn Glu Lys Ala Thr Pro Ser Asp Thr Gln Arg Ala Leu 
    210                 215                 220                 


Val Asn Leu Leu Leu Ala Glu Asp Gly Asp Lys Asp Ile Leu Lys Gln 
225                 230                 235                 240 


Gln Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Val Val Gly Leu Lys 
                245                 250                 255     


Thr Lys Leu Asn Val Ala Leu Gly Thr Glu Val Asp Ser Ser Glu Ala 
            260                 265                 270         


Thr Val Trp Asn Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp Ala Gly 
        275                 280                 285             


Ile Glu Ser Ala Met Thr Asp Glu Gly Thr Glu Ile Leu Asp Gln Ile 
    290                 295                 300                 


Arg Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Ala Gln His His Glu 
                325                 330                 335     


Asp Leu Glu Leu Phe Lys Asp Tyr Leu Lys Gln Leu Glu Asp Asp Gly 
            340                 345                 350         


Thr Ala Lys Ala Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Val Arg Glu Asp Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Asn Ala Lys Ser Pro Glu Leu Leu Glu Arg 
385                 390                 395                 400 


Leu Ala Gln Pro Asp Phe Met Leu Lys Gln Arg Asn Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Gln Ile Ile Lys 
            420                 425                 430         


Asn Gln Ser Val Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Lys Ser Met Pro Tyr Gln Leu Asp Glu Leu Leu Asn Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Ala Lys Glu Gln Lys 
465                 470                 475                 480 


Ala Ala Gln Gly Gly Val Phe Ala Trp Met Val Arg Lys Asp Pro Asp 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Arg Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asn Glu Lys Leu 
545                 550                 555                 560 


Ser Ile Glu Gln Lys Gln Gln Val Ile Arg Glu Leu Phe Glu Arg His 
                565                 570                 575     


Asn Ser Val Thr Ile Lys Gln Phe Ala Glu Asn Leu Arg Ala His Gly 
            580                 585                 590         


Asp Tyr Ala His Ile Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr Arg Gln Leu Lys Ser Leu Leu Pro 
    610                 615                 620                 


Asp Ala Ile Asp Asp Pro Ala Lys Gln Ala Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Ala Trp Ser Thr Val Phe Glu Asp Ala Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Lys Glu Ile Asn Trp Leu Asp Ser Gln Thr Ile Thr Lys Leu Ser Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser His Lys Phe Leu Asn Gly 
        675                 680                 685             


Leu Thr Leu Gly Asn Gly His Thr Ile Ile Gln Glu Leu Phe Leu Ser 
    690                 695                 700                 


Asn Asn Asn Leu Met Gln Ile Leu Thr Asp Glu Thr Leu Gln Lys Lys 
705                 710                 715                 720 


Met Thr Glu Leu Asn Ala Asp Lys Leu Lys Ala Ala Asn Ile Asn Asp 
                725                 730                 735     


Ala Ile Asp Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Arg Val Ile Glu Asp Ile Lys Arg Ala Ala Asp Gly Gln 
        755                 760                 765             


Asp Pro Ser Trp Leu Tyr Val Glu Thr Ala Asp Gly Gly Gly Thr Pro 
    770                 775                 780                 


Gly Lys Arg Thr Arg Ala Arg Gln His Gln Leu Gln Glu Ile Tyr Ala 
785                 790                 795                 800 


Asn Ala Ala His Glu Leu Ile Asp Ser Ala Val Arg Gly Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Ala Asp Lys Ala Asp Phe Asn Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Thr Pro Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val His Ala Ile Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Ala Thr Phe Ala Ser Thr Ile Tyr Ala Gln Lys 
                885                 890                 895     


Met Asn Ala Thr Trp Arg Gln Trp His Glu Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Arg Asn Leu Gln Met Arg Pro Asp Gln Ile Asp Lys Tyr 
        915                 920                 925             


Ala Ser Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Val Ala Ala Gln Tyr Pro Glu Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu Leu Glu 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Phe Pro Asn 
        995                 1000                 1005             


Leu Gln  Ala Phe Phe Thr Tyr  Gly Lys Phe Lys Lys  Ala Asp Val 
    1010                 1015                 1020             


Lys Lys  Leu Arg Gly Phe Asn  Phe Ile Arg Asp Ile  Thr His Ala 
    1025                 1030                 1035             


Glu Asp  Lys Ile Val Val Lys  Asp Thr Gly Glu Val  Ile Trp Asp 
    1040                 1045                 1050             


Lys Lys  Arg Asp Val Asp Glu  Leu Asp Arg Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val His Phe Glu Lys  Ala Asp Leu 
    1070                 1075                 1080             


Phe Lys  Gln Thr Val Tyr Gly  Ala Lys Asp Ser Lys  Glu Ala Gly 
    1085                 1090                 1095             


Gly Lys  Lys Gln Leu Ile Ser  Lys Lys Lys Gly Tyr  Pro Val Asp 
    1100                 1105                 1110             


Ile Tyr  Gly Gly Tyr Thr Gln  Glu Thr Gly Ser Tyr  Leu Ser Val 
    1115                 1120                 1125             


Val Arg  Leu Thr Lys Lys Ala  Met Tyr Ala Val Val  Lys Val Ser 
    1130                 1135                 1140             


Thr Arg  Asp Ala Ala Lys Leu  Ala Val Ala Lys Ser  Ile Ser Glu 
    1145                 1150                 1155             


Gln Lys  Glu Asn Glu Thr Leu  Asn Asp Ile Ile Asp  Glu Lys Leu 
    1160                 1165                 1170             


Ser Lys  Ile Ser Lys Asn Gly  Lys Ile Thr His Glu  Leu Phe Glu 
    1175                 1180                 1185             


Val Val  Leu Pro Arg Val Gly  Gln Lys Thr Leu Phe  Lys Asn Ser 
    1190                 1195                 1200             


Lys Tyr  Asn Leu Phe Leu Val  Asn Ser Asp Thr Tyr  Met His Asn 
    1205                 1210                 1215             


Tyr Gln  Glu Leu Trp Met Pro  Arg Glu Tyr Gln Lys  Met Trp Lys 
    1220                 1225                 1230             


Asp Ile  Ser Leu Ser Lys Tyr  Gly Asp Ala Gln Thr  Glu Asp Gln 
    1235                 1240                 1245             


Leu Asp  Gln Ile Phe Gly Phe  Ile Val Ser Gln Val  Asn Ser Tyr 
    1250                 1255                 1260             


Phe Asn  Leu Tyr Asp Ile Asn  Gln Phe Arg Lys Lys  Leu Asn Asp 
    1265                 1270                 1275             


Ala Ala  Asp Lys Phe Ala Thr  Leu Pro Met Arg Asp  Thr Asp Asp 
    1280                 1285                 1290             


Met Gln  Gly Lys Ile Ala Thr  Ile Gly Gln Leu Leu  Ile Gly Leu 
    1295                 1300                 1305             


Gln Ala  Asn Ala Ala Arg Ser  Asp Leu Arg Asn Leu  Asp Ile Lys 
    1310                 1315                 1320             


Thr Pro  Leu Gly Leu Leu Gln  Ile Pro Asn Gly Ile  Ser Leu Asp 
    1325                 1330                 1335             


Ser Asp  Thr Ser Val Val Tyr  Gln Ser Pro Thr Gly  Leu Leu Glu 
    1340                 1345                 1350             


Arg Glu  Val His Leu Ser Asp  Leu 
    1355                 1360     


<210>  950
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  950

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Met 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Asp Ile Phe Ser Pro Glu Leu Asn Gln Val Asp Glu Ser 
                85                  90                  95      


Phe Leu His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         


Gly Glu Arg His Val Ile Phe Gly Asn Ile Ala Asp Glu Val Lys Tyr 
        115                 120                 125             


His Lys Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Asp Glu Pro Ile Asp Ile 
                165                 170                 175     


Arg Asn Met Asn Ser Gln Asn Leu Phe Lys Glu Phe Leu Leu Ala Phe 
            180                 185                 190         


Asp Gly Ile Gln Val Asp Cys Tyr Leu Ala Ser Lys His Thr Asp Ile 
        195                 200                 205             


Ser Gly Ile Ile Thr Ala Lys Ile Ser Lys Ser Arg Lys Val Glu Ala 
    210                 215                 220                 


Val Leu Glu Gln Phe Pro Asp Gln Lys Lys Asn Ser Phe Phe Gly Asn 
225                 230                 235                 240 


Met Val Ser Leu Val Phe Gly Leu Met Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Glu Leu Asp Glu Asp Ala Lys Leu Gln Phe Ser Arg Asp Ser Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Asn Leu Leu Gly Ile Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             


Val Phe Val Ala Ala Lys Lys Val Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Thr Asn Asn His Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Asp Arg Tyr Asp Glu His Asn Ser Asp Lys Lys Leu Leu Arg 
                325                 330                 335     


Asp Phe Ile Arg Thr Asn Ile Gly Lys Glu Val Phe Lys Glu Val Phe 
            340                 345                 350         


Tyr Asp Thr Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Gln Lys Val 
    370                 375                 380                 


Asp Gly Gly Asp Tyr Phe Ile Glu Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Asp Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Phe Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe Lys 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
    450                 455                 460                 


Trp Ala Ser Tyr Asn Ser Asn Glu Lys Met Thr Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Asn Val Ile Asp Lys Thr Ser Ser Ala Gln Ala Phe Ile Glu Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Gln Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile 
        515                 520                 525             


Lys Tyr Val Thr Glu Thr Gly Glu Ala Arg Leu Phe Asp Val Phe Leu 
    530                 535                 540                 


Lys Lys Glu Ile Phe Asp Gly Leu Phe Lys Lys Glu Arg Lys Val Thr 
545                 550                 555                 560 


Lys Lys Lys Ile Leu Asn Phe Leu Asp Lys Asn Phe Asp Glu Phe Arg 
                565                 570                 575     


Ile Thr Asp Ile Gln Gly Leu Asp Asn Glu Thr Gly Asn Phe Asn Ala 
            580                 585                 590         


Ser Tyr Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Gly Asp Lys Glu 
        595                 600                 605             


Phe Met Asp Ser Ser Asp Asn Val Asp Val Leu Glu Asp Ile Val Leu 
    610                 615                 620                 


Ser Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Leu 
625                 630                 635                 640 


Lys Tyr Glu Asp Ile Phe Ser Lys Lys Val Ile Ala Asn Leu Thr Arg 
                645                 650                 655     


Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly 
            660                 665                 670         


Ile Lys Asp Lys His Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp 
        675                 680                 685             


Asp Gly His Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn 
    690                 695                 700                 


Leu Ser Phe Lys Asp Glu Ile Ala Asn Ser Gln Val Ile Gly Asp Gly 
705                 710                 715                 720 


Asp Asp Leu His Gln Val Val Gln Glu Leu Ala Gly Ser Pro Ala Ile 
                725                 730                 735     


Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys 
            740                 745                 750         


Val Met Gly Tyr Asn Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu 
        755                 760                 765             


Asn Gln Thr Thr Ala Arg Gly Arg Asn Asn Ser Gln Gln Arg Leu Gly 
    770                 775                 780                 


Ser Leu Thr Lys Ala Ile Gln Asp Phe Gly Ser Asp Ile Leu Lys Arg 
785                 790                 795                 800 


Tyr Pro Val Glu Asn Asn Gln Leu Gln Asn Asp Gln Leu Tyr Leu Tyr 
                805                 810                 815     


Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Asp Thr Leu Asp Ile 
            820                 825                 830         


His Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe 
        835                 840                 845             


Ile Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Thr Asn Ser Lys Ser 
    850                 855                 860                 


Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Asn Glu Val Val Lys Arg 
865                 870                 875                 880 


Met Lys Gly Phe Trp Leu Lys Gln Leu Asp Ala Lys Leu Ile Ser Gln 
                885                 890                 895     


Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Ala 
            900                 905                 910         


Glu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln 
        915                 920                 925             


Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Arg Asp 
    930                 935                 940                 


Phe Asp Lys Asn Asp Lys Arg Ile Arg Asn Val Lys Ile Val Thr Leu 
945                 950                 955                 960 


Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly Phe Tyr Lys 
                965                 970                 975     


Val Arg Glu Ile Asn Asn Phe His His Ala His Asp Ala Tyr Leu Asn 
            980                 985                 990         


Ala Val Val Ala Lys Ala Leu Leu  Ile Arg Tyr Pro Lys  Leu Glu Pro 
        995                 1000                 1005             


Glu Phe  Val Tyr Gly Glu Tyr  Pro Lys Tyr Asn Ser  Tyr Arg Glu 
    1010                 1015                 1020             


Arg Lys  Ser Ala Thr Glu Lys  Met Phe Phe Tyr Ser  Asn Ile Met 
    1025                 1030                 1035             


Asn Met  Phe Lys Thr Thr Ile  Lys Leu Ala Asp Gly  Arg Val Val 
    1040                 1045                 1050             


Glu Lys  Pro Val Ile Glu Ala  Asn Glu Glu Thr Gly  Glu Ile Ala 
    1055                 1060                 1065             


Trp Asp  Lys Thr Lys His Phe  Ala Asn Val Lys Lys  Val Leu Ser 
    1070                 1075                 1080             


Tyr Pro  Gln Val Ser Ile Val  Lys Lys Val Glu Glu  Gln Thr Gly 
    1085                 1090                 1095             


Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys Gly Gly  Ser Asp Lys 
    1100                 1105                 1110             


Leu Ile  Ala Arg Lys Thr Lys  Asn Asn Tyr Leu Ser  Thr Gln Lys 
    1115                 1120                 1125             


Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala Tyr Ser  Ile Met Phe 
    1130                 1135                 1140             


Val Ala  Asp Ile Glu Lys Gly  Lys Ser Lys Arg Leu  Lys Thr Val 
    1145                 1150                 1155             


Lys Glu  Met Ile Gly Ile Thr  Ile Met Glu Arg Ser  Arg Phe Glu 
    1160                 1165                 1170             


Ser Asn  Ser Val Thr Phe Leu  Glu Glu Lys Gly Tyr  Arg Asn Ile 
    1175                 1180                 1185             


Arg Glu  Asn Thr Ile Ile Lys  Phe Pro Lys Tyr Ser  Leu Phe Glu 
    1190                 1195                 1200             


Leu Glu  Asn Gly Arg Arg Arg  Leu Leu Ala Ser Ala  Ile Glu Leu 
    1205                 1210                 1215             


Gln Lys  Gly Asn Glu Met Phe  Leu Pro Gln Gln Phe  Val Asn Leu 
    1220                 1225                 1230             


Leu Tyr  His Ala Gln His Ala  Asn Lys Glu Asp Ser  Val Ile Tyr 
    1235                 1240                 1245             


Leu Glu  Lys His Arg His Glu  Leu Ser Glu Leu Phe  His His Ile 
    1250                 1255                 1260             


Ile Gly  Val Ser Glu Lys Thr  Ile Leu Lys Pro Lys  Val Glu Met 
    1265                 1270                 1275             


Thr Leu  Asn Glu Ala Phe Glu  Lys His Phe Glu Phe  Asp Glu Val 
    1280                 1285                 1290             


Ser Glu  Leu Ala Gln Ser Phe  Ile Ser Leu Leu Lys  Phe Thr Ala 
    1295                 1300                 1305             


Phe Gly  Ala Pro Gly Gly Phe  Lys Phe Leu Asp Ala  Asp Ile Lys 
    1310                 1315                 1320             


Gln Ser  Asn Leu Arg Tyr Gln  Thr Val Thr Glu Val  Leu Ser Ser 
    1325                 1330                 1335             


Thr Leu  Ile His Gln Ser Val  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1340                 1345                 1350             


Asp Leu  Ser Lys Leu Gly Gly  Glu 
    1355                 1360     


<210>  951
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  951

Met Gly Asn Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Thr Asp Glu Asn Tyr Asn Ile Glu Arg Phe Lys Lys Lys 
            20                  25                  30          


Asn Met Trp Gly Ile Arg Leu Phe Asp Glu Ala Lys Thr Ala Glu Ser 
        35                  40                  45              


Arg Arg Ile His Arg Gln Ala Arg Arg Met Arg Gln Arg Lys Ile Gln 
    50                  55                  60                  


Arg Leu Asn Leu Leu Met Glu Leu Phe Asp Val Glu Ile Ser Lys Val 
65                  70                  75                  80  


Asp Glu Ser Phe Phe Ile Arg Lys Lys Glu Ser Asp Leu His Tyr Glu 
                85                  90                  95      


Asp Lys Ser Gln Asp Ser Arg Tyr Ala Leu Phe Asn Asp Lys Glu Phe 
            100                 105                 110         


Asn Asp Lys Asp Tyr His Asn Lys Tyr Pro Thr Ile Phe His Leu Ile 
        115                 120                 125             


Met Asp Leu Ile Glu Asn Asp Glu Lys Lys Asp Ile Arg Leu Leu Tyr 
    130                 135                 140                 


Leu Ala Cys His Tyr Leu Ile Lys Asn Arg Gly His Phe Ile Phe Glu 
145                 150                 155                 160 


Gly Gln Lys Phe Asp Val Asn Ser Ser Leu Asn Asn Asn Ile Asn Lys 
                165                 170                 175     


Leu Lys Ser His Leu Asp Gln Phe Tyr Asn Ile Cys Leu Asp Phe Asp 
            180                 185                 190         


Asn Asp Lys Leu Ile Glu Ile Leu Val Asn Gln Asn Leu Asn Ile Ser 
        195                 200                 205             


Glu Lys Ser Lys Lys Ile Lys Glu Leu Leu Arg Lys Thr Asn Leu Glu 
    210                 215                 220                 


Val Gly Ile Ala Asn Leu Ile Ala Gly Gly Ser Ile Lys Leu Ser Asn 
225                 230                 235                 240 


Met Phe Asp Asp Asp Glu Leu Lys Asn Leu Asp Tyr Asn Ser Leu Ser 
                245                 250                 255     


Phe Lys Asn Ser Asn Phe Asp Glu Gly Arg Glu Leu Phe Glu Glu Val 
            260                 265                 270         


Leu Glu Asp Arg Phe Glu Leu Val Glu Ile Leu Lys Asp Ile His Asp 
        275                 280                 285             


Ala Ser Val Leu Glu Thr Met Leu Ser Ser Ala Ser Ile Ser Pro Asp 
    290                 295                 300                 


Gly Gly Lys Tyr Ile Ser Ser Ser Met Ile Asn Ser Tyr Asn Lys His 
305                 310                 315                 320 


Lys Arg Asp Leu Lys Leu Leu Lys Lys Ile Ile Lys Lys Tyr Lys Lys 
                325                 330                 335     


Ser Glu Tyr Asp Asn Ile Phe Arg Asp Glu Asn Ile Lys Asn Asn Tyr 
            340                 345                 350         


Leu Ser Tyr Thr Arg Thr Gly Ile Asn Lys Asn Lys Lys Val Gln Ala 
        355                 360                 365             


Glu Asp Asn Thr Asp Ser Asp Gly Phe Tyr Lys Tyr Ile Val Lys Ile 
    370                 375                 380                 


Leu Lys Asp Ile Lys Glu Asn Asp Ser Asp Glu Asp Ile Lys Tyr Ile 
385                 390                 395                 400 


Leu Ser Glu Ile Asp Lys Lys Asn Phe Val Pro Lys Gln Arg Thr Lys 
                405                 410                 415     


Glu Asn Ser Val Ile Pro Tyr Gln Leu Arg Glu Ile Glu Leu Arg Lys 
            420                 425                 430         


Ile Ile Asp Asn Gln Ser Lys Tyr His Lys Phe Leu Asn Glu Val Asp 
        435                 440                 445             


Glu Lys Gly Ile Ser Val Lys Glu Lys Ile Ile Ser Leu Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Ile Gly Pro Leu Asn Pro Ser Tyr Asn Glu Glu 
465                 470                 475                 480 


Lys Asn Phe Thr Thr Ser Trp Val Lys Arg Leu Ala Lys Gly Lys Val 
                485                 490                 495     


Thr Pro Trp Asn Phe Glu Asp Met Val Asp Ile Asp Ala Ser Arg Glu 
            500                 505                 510         


Ala Phe Ile Glu Asn Lys Leu Asn Lys Cys Ser Phe Leu Leu Asp Glu 
        515                 520                 525             


Thr Val Leu Pro Lys Asp Ser Leu Leu Tyr Gln Glu Tyr Met Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Asn Leu Lys Ile Asn Gly Gln Ser Ile Asn Thr Asp 
545                 550                 555                 560 


Leu Lys Lys Ser Ile Tyr Glu Glu Leu Phe Lys Lys Tyr Lys Lys Val 
                565                 570                 575     


Thr Arg Lys Arg Leu Ile Ser Tyr Ile Lys Glu Lys Glu Asn Met Ser 
            580                 585                 590         


Gly Asp Ile Val Ile Thr Gly Ile Asp Gly Asp Phe Lys Gln Gly Leu 
        595                 600                 605             


Lys Ser Tyr Ile Ala Phe Lys Asp Ile Ile Gly Glu Lys Ile Asp Asp 
    610                 615                 620                 


Asp Ile Tyr Arg Lys Glu Val Glu Lys Ile Ile Arg Asp Ile Ser Leu 
625                 630                 635                 640 


Tyr Gly Asp Asp Lys Ser Phe Ile Gln Lys Lys Ile Lys Asn Glu Asn 
                645                 650                 655     


Lys Leu Asp Leu Ser Asp Asp Gln Ile Ser Lys Ile Ala Lys Leu Lys 
            660                 665                 670         


Phe Lys Asp Trp Gly Arg Phe Ser Lys Lys Leu Leu Val Gly Ile Lys 
        675                 680                 685             


Ala Ser Asp Lys Asn Thr Gly Glu Ile Asp Asn Ile Ile Gly Phe Met 
    690                 695                 700                 


Arg Asn Tyr Asn Tyr Asn Ile Met Gln Leu Met Ala Asn Val Phe Thr 
705                 710                 715                 720 


Phe Lys Asp Glu Ile Glu Lys Leu Asn Ser Lys Tyr Tyr Thr Asp Glu 
                725                 730                 735     


Lys Ile Asn Ile Asp Tyr Leu Asp Asp Leu Tyr Leu Ser Ser Pro Ala 
            740                 745                 750         


Lys Arg Met Leu Trp Gln Ser Phe Gln Ile Val Asp Glu Ile Lys Asn 
        755                 760                 765             


Ile Lys Asn Ser Asp Pro Lys Lys Ile Phe Ile Glu Met Ala Arg Ser 
    770                 775                 780                 


Lys Glu Asp Lys Pro Thr Arg Lys Met Ser Arg Lys Ser Arg Leu Glu 
785                 790                 795                 800 


Lys Leu Tyr Thr Asp Ser Lys Lys Gln Phe Ile Lys Leu Phe Gly Pro 
                805                 810                 815     


Asp Glu Tyr Asp Lys Leu Asn Asn Glu Ile Lys Asn Lys Ser Glu Ser 
            820                 825                 830         


Asp Phe Arg Trp Asp Asn Leu Tyr Leu Tyr Tyr Thr Gln Phe Gly Arg 
        835                 840                 845             


Cys Met Tyr Ser Leu Asp Pro Ile Asp Leu Glu Glu Leu Ser Asn Lys 
    850                 855                 860                 


Asn Leu Tyr Asp Gln Asp His Ile Tyr Pro Lys Ser Lys Ile Tyr Asp 
865                 870                 875                 880 


Asp Ser Ile Glu Asn Arg Val Leu Val Lys Lys Gln Leu Asn Leu Asp 
                885                 890                 895     


Lys Ser Asn Glu Tyr Pro Ile Pro Asp Arg Val Leu Asn Lys Asp Lys 
            900                 905                 910         


Asn Lys Leu Leu Ala Phe Trp Lys Met Leu Asn Glu Lys Glu Leu Ile 
        915                 920                 925             


Gly Asp Lys Lys Tyr Ala Arg Leu Ile Arg Lys Ser Pro Phe Thr Asp 
    930                 935                 940                 


Glu Glu Leu Gly Asp Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln 
945                 950                 955                 960 


Ala Thr Lys Glu Thr Ala Asn Met Leu Lys Arg Leu Cys Pro Asp Ser 
                965                 970                 975     


Lys Ile Val Tyr Val Lys Ala Lys Asn Val Ser Asp Phe Arg Arg Lys 
            980                 985                 990         


Phe Asp Ile Leu Lys Cys Arg Ser  Ile Asn Asp Leu His  His Ala His 
        995                 1000                 1005             


Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn Val Phe  Asp Thr Lys 
    1010                 1015                 1020             


Phe Thr  Ser Asn Pro Leu Asn  Phe Ile Lys Asn Asn  Ser Lys Asn 
    1025                 1030                 1035             


Arg Ser  Tyr Asn Leu Glu Lys  Ile Tyr Asp Tyr Asp  Val Ile Arg 
    1040                 1045                 1050             


Asn Glu  Lys Ile Ala Trp Leu  Ala Glu Arg Lys Glu  Glu Asn Gln 
    1055                 1060                 1065             


Asp Gly  Ser Ile Ala Thr Val  Leu Lys Asn Leu Ser  Lys Asn Asp 
    1070                 1075                 1080             


Ile Arg  Val Thr Arg Arg Thr  Tyr Ile Lys Cys Glu  Asp Leu Phe 
    1085                 1090                 1095             


Asp Glu  Asn Ile Val Arg Lys  Gly Asn Gly Lys Phe  Pro Ile Lys 
    1100                 1105                 1110             


Glu Asn  Asn Asn Lys Ser Asp  Thr Asn Lys Tyr Gly  Gly Tyr Ser 
    1115                 1120                 1125             


Gly Ile  Lys Asn Ala Tyr Phe  Val Leu Leu Glu Asp  Glu Lys His 
    1130                 1135                 1140             


Asn Arg  Ile Leu Glu Ala Ile  Pro Ser Phe Ile Tyr  Lys Lys Glu 
    1145                 1150                 1155             


Lys Asn  Gly Glu Lys Gly Ser  Val Ile Asn Tyr Leu  Gln Asn Ile 
    1160                 1165                 1170             


Leu Gly  Ile Lys Ser Ala Lys  Ile Leu Arg Asp Lys  Ile Lys Ile 
    1175                 1180                 1185             


Gly Ser  Leu Leu Lys Ile Asp  Lys Phe Pro Tyr Asn  Leu Ala Gly 
    1190                 1195                 1200             


Lys Ser  Gly Asn Gly Ile Leu  Ile Ser Gly Gly Val  Gln Leu Ile 
    1205                 1210                 1215             


Leu Asp  Lys Asn Thr Ser Lys  Tyr Ile Lys Lys Ile  Glu Asn Tyr 
    1220                 1225                 1230             


Lys Tyr  Lys Leu Asp Glu Asn  Lys Asp Tyr Gln Ile  Asn Ile Phe 
    1235                 1240                 1245             


Asp Lys  Leu Ser Lys Glu Glu  Asn Ile Lys Leu Phe  Asp Leu Phe 
    1250                 1255                 1260             


Val Asp  Lys Phe Glu Asn Ser  Ile Tyr Lys Asn Arg  Val Asn Ile 
    1265                 1270                 1275             


Lys Leu  Lys Glu Ile Lys Leu  Ala Arg Pro Lys Phe  Glu His Leu 
    1280                 1285                 1290             


Ser Ile  Glu Glu Gln Ile Lys  Thr Leu Thr Gln Met  Leu Ile Leu 
    1295                 1300                 1305             


Phe Gly  Arg Glu Asn Asn Ala  Val Asn Leu Glu Tyr  Ile Gly Leu 
    1310                 1315                 1320             


Pro Lys  Asn Thr Gly Lys Thr  Lys Ile Asn Lys Lys  Ile Asn Asn 
    1325                 1330                 1335             


Lys Asp  Ile Lys Ile Ile Asn  Gln Ser Ile Thr Gly  Leu Tyr Glu 
    1340                 1345                 1350             


Thr Glu  Glu Asp Leu Ala Asn  Ile 
    1355                 1360     


<210>  952
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  952

Met Thr Lys Leu Asn Gln Pro Tyr Gly Ile Gly Leu Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Val Val Asp Ala Asn Ser His Leu Leu Arg 
            20                  25                  30          


Leu Lys Gly Glu Thr Ala Ile Gly Ala Arg Leu Phe Arg Glu Gly Gln 
        35                  40                  45              


Ser Ala Ala Glu Arg Arg Glu Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Thr Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Ala Pro His 
65                  70                  75                  80  


Ile Thr Lys Ile Asp Pro Asp Phe Phe Leu Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Asp Arg Phe Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Glu Asp Tyr Pro Ser Met Tyr 
        115                 120                 125             


His Leu Arg Leu His Leu Met Thr His Thr His Lys Ala Asp Pro Arg 
    130                 135                 140                 


Glu Ile Phe Leu Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Ala Ala Lys Asp Phe Asn Thr Asp Lys Val Asp Leu 
                165                 170                 175     


Glu Asp Ile Phe Pro Ala Leu Thr Glu Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Glu Leu Thr Phe Asp Leu Ala Lys Ala Asp Asp Phe Lys Ala 
        195                 200                 205             


Lys Leu Leu Asp Glu Gln Ala Thr Pro Ser Asp Thr Gln Lys Ala Leu 
    210                 215                 220                 


Val Asn Leu Leu Leu Ser Ser Asp Gly Glu Lys Glu Ile Val Lys Lys 
225                 230                 235                 240 


Arg Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Ile Thr Gly Leu Lys 
                245                 250                 255     


Thr Lys Phe Asn Leu Ala Leu Gly Thr Glu Val Asp Glu Ala Asp Ala 
            260                 265                 270         


Ser Asn Trp Gln Phe Ser Met Gly Gln Leu Asp Asp Lys Trp Ser Asn 
        275                 280                 285             


Ile Glu Thr Ser Met Thr Asp Gln Gly Thr Glu Ile Phe Glu Gln Ile 
    290                 295                 300                 


Gln Glu Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Met Ser Leu Ser Gln Ala Lys Val Ala Asp Tyr Gly Gln His Lys Glu 
                325                 330                 335     


Asp Leu Glu Leu Phe Lys Thr Tyr Leu Lys Lys Leu Asn Asp His Glu 
            340                 345                 350         


Leu Ala Lys Thr Ile Arg Gly Leu Tyr Asp Arg Tyr Ile Asn Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Leu Arg Glu Asp Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Asn Glu Val Ser Glu Gln Leu Leu Asn Arg 
385                 390                 395                 400 


Met Gly Gln Ala Asn Phe Met Leu Lys Gln Arg Thr Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Leu Gln Gln Arg Glu Leu Asp Gln Ile Ile Ala 
            420                 425                 430         


Asn Gln Ser Lys Tyr Tyr Asp Trp Leu Ala Lys Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Ala Ser Lys Pro Tyr Gln Leu Asp Glu Leu Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ile Thr Pro Lys Glu Gln Ala 
465                 470                 475                 480 


Glu Ser Arg Glu Asn Val Phe Ala Trp Met Ile Arg Lys Asp Pro Ser 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Cys Pro Ala 
            500                 505                 510         


Ser Ala Asn Met Phe Ile Gln Arg Met Lys Asn Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Arg Ser Leu Leu Tyr Gln Lys Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Ser Ile Asp Gly Arg Arg Leu 
545                 550                 555                 560 


Gly Ala Asp Gln Lys Gln Arg Leu Ile Arg Glu Val Phe Glu Arg His 
                565                 570                 575     


Ser Ser Val Thr Ile Lys Gln Val Ala Glu Asn Leu Val Ala His Gly 
            580                 585                 590         


Asp Phe Ala Lys Arg Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr His Gln Leu Lys Lys Ile Leu His 
    610                 615                 620                 


Glu Ala Ile Asp Asp Pro Thr Lys Glu His Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Thr Trp Ser Thr Val Phe Glu Asp Ala Ala Ile Phe Lys Thr Glu Leu 
                645                 650                 655     


Ala Lys Ile Ser Trp Leu Asp Ser Asp Thr Ile Lys Ala Leu Ser Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Arg Lys Leu Leu Asp Gly 
        675                 680                 685             


Leu Lys Leu Gly Asn Gly His Thr Val Ile Gln Glu Leu Met Leu Ser 
    690                 695                 700                 


Asn His Asn Leu Met Gln Ile Leu Thr Asp Glu Thr Leu Lys Thr Thr 
705                 710                 715                 720 


Met Ala Glu Leu Asn Gln Asp Lys Leu Lys Ala Asp Asp Ile Glu Asp 
                725                 730                 735     


Val Ile Asn Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Arg Val Val Glu Asp Ile Lys His Ala Ala Asn Gly Gln 
        755                 760                 765             


Asp Pro Ser Trp Leu Tyr Ile Glu Thr Ala Asp Gly Pro Gly Thr Ala 
    770                 775                 780                 


Gly Lys Arg Thr Gln Ser Arg Gln Lys Gln Ile Gln Thr Val Tyr Ala 
785                 790                 795                 800 


Asn Ala Ala Gln Glu Leu Ile Asp Ser Ala Val Arg Gly Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Ala Asp Lys Ala Ser Phe Thr Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Ala Pro Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser His Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Asn Ala Thr Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Asn Val Phe Ala Ser Thr Leu Phe Ala Ser Lys 
                885                 890                 895     


Met Ala Ala Thr Trp Arg Lys Trp His Glu Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Arg Asn Leu Met Leu Arg Pro Asp Glu Ile Asp Lys Phe 
        915                 920                 925             


Ala His Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Ala Ala Ala Gln Tyr Pro Asn Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu Leu Asp 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Tyr Pro Lys 
        995                 1000                 1005             


Leu Ala  Pro Phe Phe Thr Tyr  Gly Glu Phe Ala Lys  Val Asp Val 
    1010                 1015                 1020             


Lys Lys  Phe Arg Glu Phe Asn  Phe Ile Gly Ala Leu  Thr His Ala 
    1025                 1030                 1035             


Lys Lys  Asn Ile Val Ala Lys  Asp Thr Gly Glu Ile  Val Trp Asp 
    1040                 1045                 1050             


Lys Glu  Arg Asp Ile Gln Glu  Leu Asp Arg Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val Tyr Phe Glu Thr  Ala Asp Leu 
    1070                 1075                 1080             


Phe Lys  Gln Thr Ile Tyr Ala  Ala Lys Asp Ser Lys  Glu Arg Gly 
    1085                 1090                 1095             


Gly Ser  Lys Gln Leu Ile Pro  Lys Lys Gln Gly Tyr  Pro Thr Gln 
    1100                 1105                 1110             


Val Tyr  Gly Gly Tyr Thr Arg  Glu Asn Thr Ala Tyr  Leu Ala Val 
    1115                 1120                 1125             


Val Lys  Thr Val Glu Lys Lys  Ala Asp Val Tyr Arg  Val Val Arg 
    1130                 1135                 1140             


Ile Ala  Thr Ser Gln Val Ala  Ala Leu Lys Glu Ala  Arg Ala Gln 
    1145                 1150                 1155             


Ser Thr  Val Lys Glu Arg Glu  Leu Leu Lys Lys Phe  Leu Thr Thr 
    1160                 1165                 1170             


Lys Phe  Thr Lys Ile Gly Lys  Asn Gly Lys Lys Ala  Ile Thr Pro 
    1175                 1180                 1185             


Phe Asp  Ile Val Ile Pro Arg  Val Pro Arg Glu Gln  Leu Phe His 
    1190                 1195                 1200             


Asn Lys  Arg Tyr Gly Phe Phe  Met Val Asn Ser Asp  Thr Leu Met 
    1205                 1210                 1215             


His Asn  Tyr Gln Glu Leu Trp  Met Ser Arg Ser Asp  Gln Gln Ile 
    1220                 1225                 1230             


Leu Ser  Lys Leu Val Lys Ser  Ala Lys Thr Val Glu  Asn Gly Gln 
    1235                 1240                 1245             


Val Gly  Arg Leu Phe Glu Asn  Ile Val Glu Gln Ile  Asn Lys Tyr 
    1250                 1255                 1260             


Phe Pro  Leu Tyr Asp Ile Asn  Gln Phe Arg Lys Lys  Leu Asp Gln 
    1265                 1270                 1275             


Ser Lys  Asp Ile Phe Ser Lys  Leu Pro Leu Glu Gly  Asn Gly Thr 
    1280                 1285                 1290             


Thr Ser  Gly Lys Arg Glu Thr  Ile Gln Asn Ile Leu  Thr Gly Ala 
    1295                 1300                 1305             


Gly Thr  Gly Ala Lys Asn Gly  Asp Leu Lys Asn Leu  Gly Ile Lys 
    1310                 1315                 1320             


Thr Pro  Phe Gly Phe Leu Gln  Val Pro Ser Gly Ile  Val Leu Asp 
    1325                 1330                 1335             


Asp Asp  Thr Thr Leu Ile Tyr  Gln Ser Pro Thr Gly  Met Leu Asn 
    1340                 1345                 1350             


Arg Ile  Val Arg Leu Ser Glu  Leu 
    1355                 1360     


<210>  953
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  953

Met Lys Asn Glu Glu Asn Ile Tyr Leu Gly Leu Asp Ile Gly Thr Asp 
1               5                   10                  15      


Ser Val Gly Tyr Ala Val Thr Asp Asp Lys Tyr Lys Ile Met Lys Tyr 
            20                  25                  30          


His Gly Asn Ala Ala Trp Gly Ser Thr Ile Phe Asp Ala Gly Ala Leu 
        35                  40                  45              


Gly Ala Asp Arg Arg Val Phe Arg Ser Ala Arg Arg Arg Leu Asp Arg 
    50                  55                  60                  


Arg Gln Glu Arg Ile Gln Leu Leu Gln Glu Ile Phe Ala Asn Glu Ile 
65                  70                  75                  80  


Ala Lys Val Asp Glu Arg Phe Phe Ile Arg Leu Leu Glu Ser Gly Leu 
                85                  90                  95      


Trp Arg Ala Asp Ala Glu Asp Arg Tyr Val Phe Phe Asn Asp Lys Glu 
            100                 105                 110         


Tyr Thr Asp Val Gln Tyr Met Arg Asp Tyr Pro Thr Ile His His Leu 
        115                 120                 125             


Ile Cys Glu Leu Ile Tyr Asn Pro Asn Lys His Asp Val Arg Leu Val 
    130                 135                 140                 


Tyr Leu Ala Cys Ala Trp Leu Val Thr His Arg Gly His Phe Leu Ser 
145                 150                 155                 160 


Asn Ile Asn Thr Glu Asn Ile Ala Asp Leu Val Asp Ile His Asn Val 
                165                 170                 175     


Tyr Asn Gly Phe Met Arg Phe Phe Glu Glu Asn Glu Ile Ile Cys Asp 
            180                 185                 190         


Trp Arg Glu Val Asn Val Glu Glu Leu Gly Arg Val Leu Ala Ala Asn 
        195                 200                 205             


Ile Gly Ile Asn Glu Lys Tyr Arg Asn Leu Ser Thr Val Leu Phe Ala 
    210                 215                 220                 


Thr Arg Lys Pro Asp Lys Lys Ala Lys Glu Gly Phe Pro Tyr Ser Glu 
225                 230                 235                 240 


Asp Ala Ile Val Arg Leu Leu Ala Gly Gly Thr Cys Lys Leu Lys Asp 
                245                 250                 255     


Val Tyr Ile Lys Glu Glu Tyr Asp Glu Phe Gly Ser Ile Thr Leu Gly 
            260                 265                 270         


Met Asp Asp Glu Lys Met Thr Glu Ile Met Ala Asp Ile Gly Asp Asp 
        275                 280                 285             


Phe Asp Leu Ile Lys Ala Leu Arg Ser Leu Tyr Asp Trp Gly Ile Leu 
    290                 295                 300                 


Ala Asn Ile Leu Val Asn Glu Asn Asn Ser Pro Leu Ile Ser Gln Ser 
305                 310                 315                 320 


Lys Val Lys Val Tyr Glu Gln His Arg Glu Asp Leu Ala Asn Leu Lys 
                325                 330                 335     


Tyr Leu Val His Lys Tyr Ile Pro His Lys Tyr Asn Glu Ile Phe Arg 
            340                 345                 350         


Glu Ile Arg Gly Asp Asn Tyr Val Ala Tyr Ser Tyr His Tyr Glu Leu 
        355                 360                 365             


Lys Asn Asp Glu Lys Met Gly Lys Lys Ala Asp Val Glu Lys Phe Ser 
    370                 375                 380                 


Lys Phe Leu Leu Lys Ile Leu Glu Gly Ile Ser Val Asp Glu Gln Asp 
385                 390                 395                 400 


Lys Val Val Tyr Asp Asn Met Ile Glu Arg Leu Thr Leu Arg Leu Phe 
                405                 410                 415     


Leu Pro Lys Gln Lys Asn Thr Asp Asn Arg Val Ile Pro His Gln Leu 
            420                 425                 430         


Tyr Glu Tyr Glu Leu Lys Ser Ile Leu Lys Asn Thr Lys Asn Tyr Leu 
        435                 440                 445             


Pro Phe Leu Lys Glu Lys Asp Ser Asp Gly Ile Ser Asn Glu Asp Lys 
    450                 455                 460                 


Ile Ile Ser Ile Phe Lys Phe Lys Ile Pro Tyr Phe Val Gly Pro Leu 
465                 470                 475                 480 


Asn Cys Asn Ser Gln Phe Ala Trp Ile Gln Arg Lys Ala Asp Gly Lys 
                485                 490                 495     


Ile Phe Pro Trp Asn Phe Glu Asp Lys Val Asp Phe Asp Ala Ser Glu 
            500                 505                 510         


Gln Ala Phe Ile Gln Arg Met Thr Asn Gln Cys Thr Tyr Leu Pro Glu 
        515                 520                 525             


Glu Ser Val Leu Pro Lys Gln Ser Leu Tyr Tyr Gln Lys Phe Met Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Ile Lys Ile Asp Gly Arg Lys Ile Ser Val 
545                 550                 555                 560 


Ala Met Lys Gln Glu Leu Tyr Asn Glu Ile Phe Glu Lys Lys Lys Arg 
                565                 570                 575     


Val Arg Lys Lys Asp Val Ile Asp Phe Phe Val Ser Asn Gly Tyr Leu 
            580                 585                 590         


Glu Gly Gly Lys Glu Ala Leu Ile Ser Gly Leu Asp Glu Gln Ile His 
        595                 600                 605             


Thr Asn Leu Ser Ser Phe His Ala Phe Arg Arg Leu Leu Gln Asn His 
    610                 615                 620                 


Ile Leu Ser Val Glu Asp Val Glu Ser Ile Ile Glu Arg Ala Ser Tyr 
625                 630                 635                 640 


Ser Asp Asp Lys Arg Arg Val Leu Leu Phe Leu Lys Asn Lys Tyr Ser 
                645                 650                 655     


Phe Leu Thr Asp Glu Asp Ile Lys Tyr Ile Cys Lys Ile Lys Ile Lys 
            660                 665                 670         


Asp Phe Gly Arg Leu Ser Lys Lys Phe Leu Val Asp Phe Glu Gly Thr 
        675                 680                 685             


Cys Lys Glu Thr Gly Glu Ile Ser Thr Val Leu Arg Leu Met Trp Glu 
    690                 695                 700                 


Thr Asn Asn Asn Leu Met Glu Leu Leu Ser Asp Lys Tyr Thr Phe Ala 
705                 710                 715                 720 


Asn Gly Ile Lys Glu Asn Arg Lys Lys Tyr Tyr Thr Glu Gln Gln Lys 
                725                 730                 735     


Thr Leu Asp Asp Arg Leu Asp Glu Met Tyr Ile Ser Asn Ser Val Lys 
            740                 745                 750         


Arg Thr Ile Tyr Arg Thr Leu Ser Val Val Lys Asp Val Thr Lys Ala 
        755                 760                 765             


Phe Gly Val Pro Lys Lys Ile Phe Val Glu Met Ala Arg Thr Asn Asn 
    770                 775                 780                 


Pro Asp Leu Lys Gly Lys Arg Thr Arg Thr Arg Gln Gln Gln Val Ile 
785                 790                 795                 800 


Glu Leu Tyr Ala Lys Cys Asp Glu Glu Asp Val Arg Glu Leu Lys His 
                805                 810                 815     


Gln Met Glu Ser Leu Gly Glu Tyr Val Asp Asn Lys Leu Gln Ser Asp 
            820                 825                 830         


Lys Leu Phe Leu Tyr Phe Ile Gln Phe Gly Lys Ser Ala Tyr Ser Gly 
        835                 840                 845             


Lys Met Ile Asp Leu Glu Lys Leu Met Ser Gly Ser Lys Val Tyr Asp 
    850                 855                 860                 


Ile Glu His Ile Tyr Pro Gln Ala Tyr Val Lys Asp Asp Ser Ile Ile 
865                 870                 875                 880 


Asn Asn Lys Val Leu Val Phe Ser Glu Glu Asn Gly Ala Lys Gln Asp 
                885                 890                 895     


Ile Tyr Pro Ile Lys Thr Glu Ile Arg Lys Gln Met Ser Gly Ile Trp 
            900                 905                 910         


Thr Thr Trp His His Val Gly Ala Ile Ser Asp Glu Lys Tyr Lys Arg 
        915                 920                 925             


Leu Thr Arg Ser Thr Pro Phe Asn Glu Asp Glu Lys Tyr Gly Phe Ile 
    930                 935                 940                 


Asn Arg Gln Leu Thr Glu Thr Ser Gln Ser Thr Lys Ala Ile Ala Gln 
945                 950                 955                 960 


Ile Leu Asn Glu Lys Tyr Pro Glu Thr Glu Ile Val Tyr Val Lys Ala 
                965                 970                 975     


Gly Leu Val Ser Asp Phe Arg His Glu Phe Gly Leu Cys Lys Cys Arg 
            980                 985                 990         


Ser Tyr Asn Asp Leu His His Ala  Ile Asp Ala Tyr Leu  Asn Val Val 
        995                 1000                 1005             


Val Gly  Asn Val Tyr Asn Met  Lys Phe Asn Arg Lys  Trp Phe Ser 
    1010                 1015                 1020             


Val Asp  Ser Lys Tyr Ser Ile  Lys Thr Lys Thr Ile  Phe Ser His 
    1025                 1030                 1035             


Ser Leu  Val Cys Asn Gly Lys  Ile Ile Trp Asp Lys  Glu Lys Met 
    1040                 1045                 1050             


Leu Pro  Met Val Leu Lys Asn  Ala Lys Lys Asn Asn  Ala His Phe 
    1055                 1060                 1065             


Thr Lys  Tyr Gln Phe Met Lys  Thr Gly Gly Phe Phe  Asp Gln Met 
    1070                 1075                 1080             


Pro Cys  Lys Lys Ala Ser Gly  Leu Val Pro Arg Lys  Lys Gly Leu 
    1085                 1090                 1095             


Asp Thr  Glu Arg Tyr Gly Gly  Tyr Asn Lys Ser Gly  Ile Met Phe 
    1100                 1105                 1110             


Leu Thr  Pro Met Arg Tyr Lys  Ile Gly Lys Lys Ser  Asp Ile Ile 
    1115                 1120                 1125             


Ile Leu  Ser Ile Glu Leu Leu  Tyr Gly Lys Leu Phe  Leu Lys Asp 
    1130                 1135                 1140             


Glu Val  Phe Ala Lys Lys Tyr  Ala Glu Glu Arg Leu  Gln Lys Ile 
    1145                 1150                 1155             


Ile Gly  Lys Lys Ala Asp Glu  Val Ser Phe Pro Met  Gly Met Lys 
    1160                 1165                 1170             


Pro Trp  Lys Ile Asn Thr Val  Leu Ser Leu Asp Gly  Phe Asn Val 
    1175                 1180                 1185             


Cys Ile  Thr Gly Ile Gly Gly  Gly Gly Lys Cys Leu  Ser Ala Gln 
    1190                 1195                 1200             


Pro Ile  Met Gln Leu Ser Leu  Pro Tyr Glu Leu Ala  Lys Tyr Leu 
    1205                 1210                 1215             


Lys Lys  Ile Glu Lys Phe Val  Glu Lys Asn Lys Asn  Asn Ala Asn 
    1220                 1225                 1230             


Tyr Ile  Tyr Asp Glu Gln Tyr  Asp Ser Val Ser Val  Asp Glu Asn 
    1235                 1240                 1245             


Ile Lys  Leu Tyr Asp Val Tyr  Leu Asp Lys Leu Glu  Asn Ser Ile 
    1250                 1255                 1260             


Tyr Asn  Lys Arg Ile Asn Ser  Pro Glu Ala Ile Leu  Lys Asn Gly 
    1265                 1270                 1275             


Arg Glu  Lys Phe Cys Lys Leu  Asn Val Leu Glu Gln  Ala Val Thr 
    1280                 1285                 1290             


Leu Ile  Asn Ile Gln Ser Ile  Phe Gly Arg Met Thr  Gly Gly Cys 
    1295                 1300                 1305             


Asp Leu  Glu Asn Ile Gly Gly  Lys Lys His Ser Ala  Ala Thr Val 
    1310                 1315                 1320             


Asn Phe  Ser Ser Asn Val Ser  Asn Trp Lys Lys Tyr  Tyr Asn Asp 
    1325                 1330                 1335             


Val Arg  Val Val Tyr Gln Ser  Pro Ser Gly Ile Trp  Glu Arg Arg 
    1340                 1345                 1350             


Ser Gln  Asn Ile Leu Glu Leu  Leu 
    1355                 1360     


<210>  954
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  954

Met Asn Glu Pro Tyr Gly Val Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Thr Val Val Asp Met Asn Gly Arg Val Arg Lys Val Lys Gly 
            20                  25                  30          


Lys Thr Ala Leu Gly Ala Arg Leu Phe Lys Glu Gly Ala Thr Ala Glu 
        35                  40                  45              


Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Val Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Phe Phe Asp Gln Pro Ile Ser Lys 
65                  70                  75                  80  


Ile Asp Pro Asn Phe Phe Ala Arg Arg Lys Tyr Ser Asp Ile Ser Pro 
                85                  90                  95      


Arg Asp Pro Asn Tyr Asn Gly Leu Ala Lys Thr Leu Phe Asn Asp Arg 
            100                 105                 110         


Thr Asp Lys Glu Phe Tyr Asp Asp Tyr Ala Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Asp Lys Leu Met Thr Ser Asn Arg Lys Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Arg Thr 
145                 150                 155                 160 


Gly Pro Ala Ser Gln Tyr Gly Ser Ala Ser Leu His Leu Ala Thr Ser 
                165                 170                 175     


Phe Gln Lys Leu Asn Asp Leu Phe Ala Gln Ser Glu Glu Thr Leu Asn 
            180                 185                 190         


Leu Lys Leu Val Thr Asp Glu Ala Leu Leu Gln Gln Ile Gln Gln Ile 
        195                 200                 205             


Leu Val Arg Thr Asp Leu Ser Arg Ser Glu Gln Gln Arg Gln Ile Trp 
    210                 215                 220                 


Pro Leu Met Ala Val Leu Thr Gly Ala Ser Ala Ala Glu Lys Lys Arg 
225                 230                 235                 240 


Gln Lys Asn Val Val Val Glu Leu Ser Lys Ala Leu Val Gly Leu Lys 
                245                 250                 255     


Ala Lys Met Asn Val Val Thr Leu Thr Glu Val Asp Ala Ala Val Val 
            260                 265                 270         


Lys Asp Trp Thr Phe Thr Met Glu Glu Ser Gln Asp Lys Leu Pro Glu 
        275                 280                 285             


Ile Glu Glu Gln Leu Ser Glu Val Gly Gln Gln Ile Met Asp Glu Val 
    290                 295                 300                 


Ile Gln Leu Tyr Ala Ser Val Asn Leu Ala Gln Leu Ile Pro Ala Gly 
305                 310                 315                 320 


Lys Arg Phe Ser Gln His Met Val Glu Lys Tyr Lys His His Glu Lys 
                325                 330                 335     


Asn Leu Glu Leu Leu Lys Ala Tyr Ile His Ser Gln Ser Asp Ser Lys 
            340                 345                 350         


Arg Gly Arg Glu Ile Arg Ala Thr Tyr Asp Arg Tyr Ile Asp Gly Val 
        355                 360                 365             


Asp Ser Lys Pro Val Thr Gln Glu Met Phe Tyr Lys Asp Leu Met Lys 
    370                 375                 380                 


Tyr Val Glu Ala Asp Ala Thr Ser Asn His Leu Ala Ala Glu Ile Lys 
385                 390                 395                 400 


Asp Glu Ile Asp Ser Glu Gln Phe Met Pro Lys Leu Arg Thr Lys Gln 
                405                 410                 415     


Asn Gly Ser Ile Pro Tyr Gln Val Gln Gln Tyr Glu Leu Asp Gln Ile 
            420                 425                 430         


Ile Ser His Gln Lys Lys Tyr Tyr Pro Trp Leu Gly Glu Glu Asn Pro 
        435                 440                 445             


Val Ala Glu Arg Arg Gly Lys Phe Pro Tyr Lys Leu Asp Glu Leu Val 
    450                 455                 460                 


Gly Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr Lys Glu Asp 
465                 470                 475                 480 


Gln Gln Ala Thr Ser Gly Ala Gly Phe Ala Trp Met Val Arg Lys Ala 
                485                 490                 495     


Asp Gly Pro Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Ile 
            500                 505                 510         


Ala Ser Ala Thr Ala Phe Ile Gln Arg Met Gln Thr Thr Asp Thr Tyr 
        515                 520                 525             


Leu Ile Gly Glu Asp Val Leu Pro Ala Arg Ser Leu Ile Tyr Gln Arg 
    530                 535                 540                 


Phe Met Val Leu Asn Glu Leu Asn Asn Met Arg Val Glu Asp Arg Lys 
545                 550                 555                 560 


Leu Ala Pro Gln Gln Lys Gln Arg Leu Tyr Asn Gln Val Phe Lys Gln 
                565                 570                 575     


His Gln His Val Ser Val Lys Asn Ile Gln Gln Asn Leu Met Asp Ala 
            580                 585                 590         


Gly Glu Tyr Arg Lys Thr Pro Gln Ile Thr Gly Leu Ala Asp Pro Lys 
        595                 600                 605             


Gly Phe Asn Ser Ser Leu Ser Thr Tyr His Asp Phe Lys Lys Ile Leu 
    610                 615                 620                 


Leu Glu Ala Ile Ala Asp Glu His Lys Arg Ala Asp Ile Glu Lys Ile 
625                 630                 635                 640 


Ile Leu Trp Ser Thr Thr Phe Glu Asp Ser Ala Ile Phe Lys Gln Lys 
                645                 650                 655     


Leu Glu Glu Val Ala Trp Leu Thr Asp Ala Gln Arg Lys Gln Leu Ser 
            660                 665                 670         


Gly Leu Arg Tyr Arg Gly Trp Gly Gln Leu Ser His Lys Leu Leu Thr 
        675                 680                 685             


Ala Phe Lys Asp Asp Lys Gly Arg Ser Ile Met Asp Gly Leu Trp Glu 
    690                 695                 700                 


Thr Ser Asp Asn Phe Met Gln Leu Arg Lys Gln Pro Ala Ile Glu Thr 
705                 710                 715                 720 


Gln Ile Lys Glu Ala Asn Gln Ala Asn Leu Thr Asp Ala Asp Ile Gln 
                725                 730                 735     


Asp Thr Ile Asn Glu Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile 
            740                 745                 750         


Arg Glu Val Met Leu Val Leu Asp Asp Ile Lys Asn Ala Met His Gly 
        755                 760                 765             


Gln Thr Pro Ser Trp Ile Phe Val Glu Ala Ala Arg Gly Gly Gly Val 
    770                 775                 780                 


Ala Gly Arg Arg Thr Gln Ser Arg Ser Ser Gln Ile Val Glu Ala Tyr 
785                 790                 795                 800 


Lys Gly Thr Ala Lys Glu Ile Val Ser Glu Lys Val Gln His Glu Leu 
                805                 810                 815     


Asn Glu Lys Ile Lys Ala Lys Ala Asp Phe Asn Thr Arg Leu Val Leu 
            820                 825                 830         


Tyr Phe Leu Gln Asn Gly Arg Asp Leu Tyr Thr Asn Glu Ala Ile Asn 
        835                 840                 845             


Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Leu Pro Gln Ser 
    850                 855                 860                 


Leu Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ala Arg 
865                 870                 875                 880 


Ile Asn Arg Glu Lys Asn Asp Thr Phe Ala Ser Glu Lys Phe Gly Arg 
                885                 890                 895     


Lys Met Gly Ala Gln Trp Arg Glu Leu His Arg Asn Gly Leu Met Thr 
            900                 905                 910         


Gln Arg Lys Leu Lys His Leu Leu Met Arg Pro Asp Glu Ile Ser Lys 
        915                 920                 925             


His Ala Thr Asp Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val 
    930                 935                 940                 


Ile Lys Leu Val Glu Glu Leu Ile Ser Ser Glu Tyr Pro Ala Ala Ser 
945                 950                 955                 960 


Ile Val Ala Val Lys Ala Asn Leu Thr His Gln Phe Arg Gln Thr Phe 
                965                 970                 975     


Asn Phe Pro Lys Leu Arg Glu Val Asn Asp Tyr His His Ala Phe Asp 
            980                 985                 990         


Ala Ser Leu Thr Ala Phe Ile Gly  Met Tyr Leu Leu Lys  Gln Tyr Pro 
        995                 1000                 1005             


Lys Leu  Glu Arg Phe Phe Val  Tyr Gly Lys Phe Ala  Lys Gln Pro 
    1010                 1015                 1020             


Ile Asn  Leu Thr Arg Phe Asn  Ile Ile Arg Lys Leu  Ala Val Ala 
    1025                 1030                 1035             


Glu Lys  Pro Ile Ala Asn Ile  Glu Thr Gly Glu Ile  Leu Trp Asp 
    1040                 1045                 1050             


Lys Thr  Ala Asp Ile Lys Tyr  Phe Glu Lys Leu Tyr  Asn Tyr Lys 
    1055                 1060                 1065             


Arg Leu  Leu Val Thr His Glu  Val Arg Glu Asn Tyr  Gly Ala Met 
    1070                 1075                 1080             


Phe Lys  Gln Thr Leu Phe Lys  Ala Ser Tyr Asn Lys  Ser Lys Thr 
    1085                 1090                 1095             


Leu Val  Pro Lys Lys Asn His  Met Glu Thr Ser Val  Tyr Gly Gly 
    1100                 1105                 1110             


Tyr Ser  Asn Gln Glu Thr Ala  Tyr Leu Ala Ile Val  Arg Ile Pro 
    1115                 1120                 1125             


Phe Lys  Ser Gly Phe Lys Phe  Ile Val Val Gly Ile  Pro Thr Arg 
    1130                 1135                 1140             


Met Val  Ala Lys Ile Lys His  Tyr Gln Ser Leu Gly  Ala Thr Leu 
    1145                 1150                 1155             


Asn Gln  Ala Thr His Lys Val  Ile Glu Pro Lys Phe  Thr Lys Ile 
    1160                 1165                 1170             


Ser Arg  Lys Thr Lys Gln Thr  Val Ile Ser Asp Tyr  Glu Val Val 
    1175                 1180                 1185             


Leu Pro  Lys Val Tyr Leu Asp  Gln Val Val Arg Asp  Gln Val Lys 
    1190                 1195                 1200             


Gly Gln  Met Tyr Arg Phe Ser  Leu Gly Ser Asp Lys  Glu Tyr His 
    1205                 1210                 1215             


Asn Val  Gln Glu Leu Tyr Leu  Pro Leu Ser Ile Gln  Gln Ala Phe 
    1220                 1225                 1230             


Val Gly  His Tyr Asp Glu Ser  Asp Asp Gln Arg Ser  Asn Asp Leu 
    1235                 1240                 1245             


Val Lys  Val Tyr Asp Ala Ala  Leu Lys Gln Leu Gln  Arg Tyr Phe 
    1250                 1255                 1260             


Pro Leu  His Leu Ser Arg Asn  Phe Asp Gln Val Ala  Ser Gln Ala 
    1265                 1270                 1275             


His Gln  Ser Phe Glu Gly Leu  Lys Asn Asn Val Gln  Thr Ser Asp 
    1280                 1285                 1290             


Lys Gln  Leu Gly Lys Lys Glu  Val Leu Asn Ser Leu  Phe Val Gly 
    1295                 1300                 1305             


Leu His  Ala Asn Ala Thr Arg  Ser Asn Leu Ser Val  Leu Gly Met 
    1310                 1315                 1320             


Ser Lys  Asp Phe Gly Arg Ile  Lys Ser Asn Gly Ile  Thr Leu Thr 
    1325                 1330                 1335             


Asp Gln  Ala Glu Ile Ile Tyr  Gln Ser Pro Thr Gly  Leu Phe Glu 
    1340                 1345                 1350             


Arg Lys  Val Ala Leu Lys Asp  Leu 
    1355                 1360     


<210>  955
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  955

Met Gly Asn Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Thr Asp Glu Asn Tyr Asn Ile Glu Arg Phe Lys Lys Lys 
            20                  25                  30          


Asn Met Trp Gly Ile Arg Leu Phe Asp Glu Ala Lys Thr Ala Glu Ser 
        35                  40                  45              


Arg Arg Ile His Arg Gln Ala Arg Arg Met Arg Gln Arg Lys Ile Gln 
    50                  55                  60                  


Arg Leu Asn Leu Leu Met Glu Leu Phe Asp Val Glu Ile Ser Lys Val 
65                  70                  75                  80  


Asp Glu Ser Phe Phe Ile Arg Lys Lys Glu Ser Asp Leu His Tyr Glu 
                85                  90                  95      


Asp Lys Ser Gln Asp Ser Arg Tyr Ala Leu Phe Asn Asp Lys Glu Phe 
            100                 105                 110         


Asn Asp Lys Asp Tyr His Asn Lys Tyr Pro Thr Ile Phe His Leu Ile 
        115                 120                 125             


Met Asp Leu Ile Glu Asn Asp Glu Lys Lys Asp Ile Arg Leu Leu Tyr 
    130                 135                 140                 


Leu Ala Cys His Tyr Leu Ile Lys Asn Arg Gly His Phe Ile Phe Glu 
145                 150                 155                 160 


Gly Gln Lys Phe Asp Val Asn Ser Ser Leu Asn Asn Asn Ile Asn Lys 
                165                 170                 175     


Leu Lys Ser His Leu Asp Gln Phe Tyr Asn Ile Cys Leu Asp Phe Asp 
            180                 185                 190         


Asn Asp Lys Leu Ile Glu Ile Leu Val Asn Gln Asn Leu Asn Ile Ser 
        195                 200                 205             


Glu Lys Ser Lys Lys Ile Lys Glu Leu Leu Arg Lys Thr Asn Leu Glu 
    210                 215                 220                 


Val Gly Ile Ala Asn Leu Ile Ala Gly Gly Ser Ile Lys Leu Ser Asn 
225                 230                 235                 240 


Met Phe Asp Asp Asp Glu Leu Lys Asn Phe Asp Tyr Asn Ser Leu Ser 
                245                 250                 255     


Phe Lys Asn Ser Asn Phe Asp Glu Gly Arg Glu Leu Phe Glu Glu Val 
            260                 265                 270         


Leu Glu Asp Arg Phe Glu Leu Val Glu Ile Leu Lys Asp Ile His Asp 
        275                 280                 285             


Ala Ser Val Leu Glu Thr Met Leu Ser Ser Ala Ser Ile Ser Pro Asp 
    290                 295                 300                 


Gly Gly Lys Tyr Ile Ser Ser Ser Met Ile Asn Ser Tyr Asn Lys His 
305                 310                 315                 320 


Lys Arg Asp Leu Lys Leu Leu Lys Lys Ile Ile Lys Lys Tyr Lys Lys 
                325                 330                 335     


Ser Glu Tyr Asp Asn Ile Phe Arg Asp Glu Asn Ile Lys Asn Asn Tyr 
            340                 345                 350         


Leu Ser Tyr Thr Arg Thr Gly Ile Asn Lys Asn Lys Lys Val Gln Ala 
        355                 360                 365             


Lys Asp Asn Thr Asp Ser Asp Gly Phe Tyr Lys Tyr Ile Val Lys Ile 
    370                 375                 380                 


Leu Lys Asp Ile Lys Glu Asn Asp Ser Asp Glu Asp Ile Lys Tyr Ile 
385                 390                 395                 400 


Leu Ser Glu Ile Asp Lys Lys Asn Phe Val Pro Lys Gln Arg Thr Lys 
                405                 410                 415     


Glu Asn Ser Val Ile Pro Tyr Gln Leu Arg Glu Ile Glu Leu Arg Lys 
            420                 425                 430         


Ile Ile Asp Asn Gln Ser Lys Tyr His Lys Phe Leu Asn Glu Val Asp 
        435                 440                 445             


Glu Lys Gly Ile Ser Val Lys Glu Lys Ile Ile Ala Leu Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Ile Gly Pro Leu Asn Pro Ser Tyr Asn Glu Glu 
465                 470                 475                 480 


Lys Asn Phe Thr Thr Ser Trp Val Lys Arg Leu Ala Lys Gly Lys Val 
                485                 490                 495     


Thr Pro Trp Asn Phe Glu Asp Met Val Asp Ile Asp Ala Ser Arg Glu 
            500                 505                 510         


Ala Phe Ile Glu Asn Lys Leu Asn Lys Cys Ser Phe Leu Leu Asp Glu 
        515                 520                 525             


Thr Val Leu Pro Lys Asp Ser Leu Leu Tyr Gln Glu Tyr Met Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Asn Leu Lys Ile Asn Gly Gln Ser Ile Asn Thr Asp 
545                 550                 555                 560 


Leu Lys Lys Ser Ile Tyr Glu Glu Leu Phe Lys Lys Tyr Lys Lys Val 
                565                 570                 575     


Thr Arg Lys Arg Leu Ile Ser Tyr Ile Lys Glu Lys Glu Asn Thr Ser 
            580                 585                 590         


Gly Asp Ile Val Ile Thr Gly Ile Asp Gly Asp Phe Lys Gln Gly Leu 
        595                 600                 605             


Lys Ser Tyr Ile Ala Phe Lys Glu Ile Ile Gly Glu Lys Ile Asp Asn 
    610                 615                 620                 


Asp Ile Tyr Arg Lys Glu Val Glu Lys Ile Ile Arg Asp Ile Ser Leu 
625                 630                 635                 640 


Tyr Gly Asp Asp Lys Ser Phe Ile Gln Lys Lys Ile Lys Asn Glu Asn 
                645                 650                 655     


Lys Leu Asp Leu Ser Asp Asn Gln Ile Ser Lys Ile Ala Lys Leu Lys 
            660                 665                 670         


Phe Lys Asp Trp Gly Arg Phe Ser Lys Lys Leu Leu Val Gly Ile Lys 
        675                 680                 685             


Ala Ser Asp Lys Asn Thr Gly Glu Ile Asp Asn Ile Ile Gly Phe Met 
    690                 695                 700                 


Arg Asn Tyr Asn Tyr Asn Ile Met Gln Leu Met Ala Asn Val Phe Thr 
705                 710                 715                 720 


Phe Lys Asp Glu Ile Glu Lys Leu Asn Ser Lys Tyr Tyr Thr Tyr Glu 
                725                 730                 735     


Lys Ile Asn Val Asp Tyr Leu Asp Asp Leu Tyr Leu Ser Ala Pro Ala 
            740                 745                 750         


Lys Arg Met Leu Trp Gln Ser Phe Gln Ile Val Asp Glu Ile Lys Asn 
        755                 760                 765             


Ile Lys Asn Ser Ala Pro Lys Lys Ile Phe Ile Glu Met Ala Arg Ser 
    770                 775                 780                 


Lys Glu Asp Lys Pro Thr Arg Lys Met Ser Arg Lys Ser Arg Leu Glu 
785                 790                 795                 800 


Lys Leu Tyr Thr Asp Ser Lys Lys Gln Phe Ile Lys Leu Phe Gly Pro 
                805                 810                 815     


Asp Glu Tyr Asp Lys Leu Asn Asn Glu Ile Lys Asn Lys Ser Glu Ser 
            820                 825                 830         


Asp Phe Arg Trp Asp Asn Leu Tyr Leu Tyr Tyr Thr Gln Phe Gly Arg 
        835                 840                 845             


Cys Met Tyr Ser Leu Asp Pro Ile Asp Leu Glu Glu Leu Ser Asn Lys 
    850                 855                 860                 


Asn Leu Tyr Asp Gln Asp His Ile Tyr Pro Lys Ser Lys Ile Tyr Asp 
865                 870                 875                 880 


Asp Ser Ile Glu Asn Arg Val Leu Val Lys Lys Gln Leu Asn Leu Asp 
                885                 890                 895     


Lys Ser Asn Glu Tyr Pro Ile Pro Asp Arg Val Leu Asn Lys Asp Lys 
            900                 905                 910         


Asn Lys Leu Leu Ala Phe Trp Lys Met Leu Asn Glu Lys Glu Leu Ile 
        915                 920                 925             


Gly Asp Lys Lys Tyr Ala Arg Leu Ile Arg Lys Ser Pro Phe Thr Asp 
    930                 935                 940                 


Glu Glu Leu Gly Asp Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln 
945                 950                 955                 960 


Ala Thr Lys Glu Thr Ala Asn Met Leu Arg Arg Leu Cys Pro Asp Ser 
                965                 970                 975     


Lys Ile Val Tyr Val Lys Ala Lys Asn Val Ser Asp Phe Arg Arg Lys 
            980                 985                 990         


Phe Asp Ile Leu Lys Cys Arg Ser  Ile Asn Asp Leu His  His Ala His 
        995                 1000                 1005             


Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn Val Phe  Asp Thr Lys 
    1010                 1015                 1020             


Phe Thr  Ser Asn Pro Leu Asn  Phe Ile Lys Asn Asn  Ser Lys Asn 
    1025                 1030                 1035             


Arg Ser  Tyr Asn Leu Glu Lys  Ile Tyr Asp Tyr Asp  Val Ile Arg 
    1040                 1045                 1050             


Asn Glu  Lys Ile Ala Trp Leu  Ala Glu Arg Lys Glu  Glu Asn Gln 
    1055                 1060                 1065             


Asp Gly  Ser Ile Ala Thr Val  Leu Lys Asn Leu Ser  Lys Asn Asp 
    1070                 1075                 1080             


Ile Arg  Val Thr Arg Arg Thr  Tyr Ile Lys Cys Glu  Asp Leu Phe 
    1085                 1090                 1095             


Asp Glu  Asn Ile Val Arg Lys  Gly Asn Gly Lys Phe  Pro Leu Lys 
    1100                 1105                 1110             


Glu Asn  Asn Asn Lys Ser Asp  Thr Asn Lys Tyr Gly  Gly Tyr Ser 
    1115                 1120                 1125             


Gly Ile  Lys Asn Ala Tyr Phe  Val Leu Leu Glu Asp  Glu Lys His 
    1130                 1135                 1140             


Asn Arg  Ile Ile Glu Ala Ile  Pro Ser Phe Ile Tyr  Lys Lys Glu 
    1145                 1150                 1155             


Lys Asn  Gly Glu Lys Gly Ser  Val Ile Asn Tyr Leu  Gln Asn Ile 
    1160                 1165                 1170             


Leu Gly  Ile Lys Ser Ala Lys  Ile Leu Arg Asp Lys  Ile Lys Ile 
    1175                 1180                 1185             


Gly Ser  Leu Leu Lys Ile Asp  Lys Phe Pro Tyr Asn  Leu Ala Gly 
    1190                 1195                 1200             


Lys Ser  Gly Asn Gly Ile Leu  Ile Ser Gly Gly Val  Gln Leu Ile 
    1205                 1210                 1215             


Leu Asp  Lys Asn Thr Ser Lys  Tyr Ile Lys Lys Ile  Glu Asn Tyr 
    1220                 1225                 1230             


Lys Tyr  Lys Leu Asp Glu Asn  Lys Asp Tyr Gln Ile  Asn Ile Phe 
    1235                 1240                 1245             


Asp Lys  Leu Ser Lys Glu Glu  Asn Ile Lys Leu Phe  Asp Leu Phe 
    1250                 1255                 1260             


Val Asp  Lys Phe Glu Asn Ser  Ile Tyr Lys Asn Arg  Val Asn Ile 
    1265                 1270                 1275             


Lys Leu  Lys Glu Ile Lys Leu  Ala Arg Ser Lys Phe  Glu His Leu 
    1280                 1285                 1290             


Ser Ile  Glu Glu Gln Ile Lys  Thr Leu Thr Gln Met  Leu Ile Leu 
    1295                 1300                 1305             


Phe Gly  Arg Glu Asn Asn Ala  Val Asn Leu Glu Tyr  Ile Gly Leu 
    1310                 1315                 1320             


Pro Lys  Asn Thr Gly Lys Thr  Lys Ile Asn Lys Lys  Ile Asn Asn 
    1325                 1330                 1335             


Lys Asp  Ile Lys Ile Ile Asn  Gln Ser Ile Thr Gly  Leu Tyr Glu 
    1340                 1345                 1350             


Thr Glu  Glu Asp Leu Ala Asn  Ile 
    1355                 1360     


<210>  956
<211>  1362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  956

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Glu Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Ser Arg Asp Phe Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Leu Asp Glu Met Asn Lys Leu Asp Ser Gly 
                85                  90                  95      


Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Glu Lys Arg 
            100                 105                 110         


Gly Ser Arg Tyr Pro Ile Phe Gly Thr Leu Gln Glu Glu Val Asn Tyr 
        115                 120                 125             


His Glu Asn Phe Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp Ser 
                165                 170                 175     


Asn Asn Asn Ser Ile Gln Glu Ile Phe Asn Glu Phe Thr Ala Glu Tyr 
            180                 185                 190         


His Asn Thr Ile Glu Gly Ser Cys Leu Ser Gly Gln Ile Glu Gln Val 
        195                 200                 205             


Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 


Ile Leu Lys Leu Phe Pro Ala Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     


Asp Leu Glu Glu Lys Thr Pro Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Asp Leu Glu Asn Leu Leu Glu Gln Ile Gly Asp Glu Phe Ala Asp 
        275                 280                 285             


Leu Phe Leu Leu Ala Lys Lys Leu Tyr Asp Ala Thr Leu Leu Ala Gly 
    290                 295                 300                 


Ile Leu Thr Ala Thr Asp Val Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Asn Arg Tyr Glu Thr His Gln Gln Asp Leu Ala Ala Leu Lys 
                325                 330                 335     


Gln Phe Ile Lys Asn Lys Leu Pro Glu Lys Tyr Ser Glu Val Phe Ser 
            340                 345                 350         


Asp Leu Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asn Gly Lys Thr Thr 
        355                 360                 365             


Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ile Lys Phe Glu 
    370                 375                 380                 


Gly Ala Val Asp Phe Leu Glu Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Leu His Leu 
                405                 410                 415     


Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Asp His Tyr Pro Phe 
            420                 425                 430         


Leu Lys Glu Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 


Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Thr Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     


Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Ile Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 


Lys Lys Gln Ile Ile Asn Leu Leu Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 


Glu Lys Asp Ile Ile His Tyr Leu His Thr Val Asp Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Asp Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Met Asp Asp 
        595                 600                 605             


Ala Asp Asn Glu Ala Ile Leu Glu Asn Ile Val His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Gln Glu Met Ile Lys Gln Ser Leu Ala Gln Tyr Asp Ala 
625                 630                 635                 640 


Ile Phe Asp Arg Lys Val Met Lys Ala Leu Thr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Glu Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         


Gln Thr Gly Lys Met Ile Leu Glu Tyr Leu Lys Asp Asp Gly Glu Ile 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys 
    690                 695                 700                 


Glu Thr Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Glu Asn Ile Lys 
705                 710                 715                 720 


Gln Ile Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         


Asn Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln Phe Thr 
        755                 760                 765             


Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Lys Leu Thr Asp 
    770                 775                 780                 


Ser Ile Asn Lys Leu Gly Ser Ser Ile Leu Glu Glu His Pro Val Asp 
785                 790                 795                 800 


Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     


Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Glu Leu Ser Lys Leu Ser 
            820                 825                 830         


Ser Tyr Asp Val Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             


Ser Leu Asp Asn Arg Val Leu Val Ser Tyr Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 


Ser Asp Asp Val Pro Ser Leu Glu Ile Val Gln Lys Arg Lys Ala Phe 
865                 870                 875                 880 


Trp Arg Gln Leu Leu Asp Ser Gln Leu Ile Ser Glu Arg Lys Phe Asn 
                885                 890                 895     


Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         


Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             


Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Val Thr Glu Lys 
    930                 935                 940                 


Gly Lys Lys Lys Arg Ala Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 


Val Ser Asn Phe Arg Lys Asp Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     


Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         


Thr Ala Leu Leu Lys Lys Tyr Pro  Lys Leu Glu Pro Glu  Phe Val Tyr 
        995                 1000                 1005             


Gly Asp  Tyr Pro Lys Tyr Asn  Ser Tyr Arg Glu Arg  Lys Thr Ala 
    1010                 1015                 1020             


Thr Glu  Lys Val Tyr Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1025                 1030                 1035             


Thr Thr  Val Lys Leu Ala Asp  Gly Ser Glu Lys Lys  Arg His Gln 
    1040                 1045                 1050             


Ile Glu  Thr Asn Glu Glu Thr  Gly Glu Ile Val Trp  Asp Lys Thr 
    1055                 1060                 1065             


Lys His  Phe Glu Ile Val Arg  Lys Val Leu Ser Tyr  Pro Gln Val 
    1070                 1075                 1080             


Asn Ile  Val Lys Lys Thr Glu  Glu Gln Thr Val Gly  Gln Asn Gly 
    1085                 1090                 1095             


Gly Leu  Phe Asp Asn Asn Ile  Val Ser Lys Asn Lys  Val Val Asp 
    1100                 1105                 1110             


Ala Ser  Lys Leu Ile Pro Ile  Lys Ser Gly Leu Ser  Pro Glu Lys 
    1115                 1120                 1125             


Tyr Gly  Gly Tyr Ala Arg Pro  Thr Ile Ala Tyr Ser  Val Leu Val 
    1130                 1135                 1140             


Ile Ala  Asp Ile Glu Lys Gly  Lys Ala Lys Lys Leu  Lys Arg Ile 
    1145                 1150                 1155             


Lys Glu  Met Val Gly Ile Thr  Ile Gln Asp Lys Lys  Lys Phe Glu 
    1160                 1165                 1170             


Ala Asn  Pro Ile Ala Tyr Leu  Glu Glu Cys Gly Phe  Lys Asn Ile 
    1175                 1180                 1185             


Asn Ser  Asn His Ile Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu 
    1190                 1195                 1200             


Phe Asp  Asp Gly Gln Arg Arg  Leu Leu Ala Ser Ser  Ile Glu Leu 
    1205                 1210                 1215             


Gln Lys  Gly Asn Glu Leu Ile  Leu Pro Tyr His Leu  Thr Ala Leu 
    1220                 1225                 1230             


Leu Tyr  His Ala Gln Arg Ile  Asn Lys Ile Ser Glu  Pro Ile His 
    1235                 1240                 1245             


Lys Gln  Tyr Val Glu Ala His  Gln Asn Glu Phe Glu  Glu Leu Leu 
    1250                 1255                 1260             


Thr Thr  Ile Ile Ser Leu Ser  Lys Lys Tyr Ile Gln  Lys Pro Asn 
    1265                 1270                 1275             


Val Glu  Ser Leu Leu Gln Gln  Ala Phe Asp Gln Thr  Asp Glu Asp 
    1280                 1285                 1290             


Ile Tyr  Gln Leu Ser Glu Ser  Phe Ile Ser Leu Leu  Lys Leu Ile 
    1295                 1300                 1305             


Ser Phe  Gly Ala Pro Gly Ala  Phe Lys Phe Leu Gly  Val Asp Ile 
    1310                 1315                 1320             


Ser Gln  Ser Asn Val Arg Tyr  Gln Ser Val Ser Ser  Cys Phe Asn 
    1325                 1330                 1335             


Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1340                 1345                 1350             


Ile Asp  Leu Ser Lys Leu Gly  Glu Asp 
    1355                 1360         


<210>  957
<211>  1362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  957

Met Asn Glu Pro Tyr Gly Val Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Thr Val Val Asp Met Asn Gly Arg Val Arg Lys Val Lys Gly 
            20                  25                  30          


Lys Thr Ala Leu Gly Ala Arg Leu Phe Lys Glu Gly Ala Thr Ala Glu 
        35                  40                  45              


Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Val Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Phe Phe Asp Gln Pro Ile Ser Lys 
65                  70                  75                  80  


Ile Asp Pro Asn Phe Phe Ala Arg Arg Lys Tyr Ser Asp Ile Ser Pro 
                85                  90                  95      


Arg Asp Pro Asn Tyr Asn Gly Leu Ala Lys Thr Leu Phe Asn Asp Arg 
            100                 105                 110         


Thr Asp Lys Glu Phe Tyr Asp Asp Tyr Ala Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Asp Lys Leu Met Thr Ser Asn Arg Lys Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Arg Thr 
145                 150                 155                 160 


Gly Pro Ala Ser Gln Tyr Gly Ser Ala Ser Leu His Leu Ala Thr Ser 
                165                 170                 175     


Phe Gln Lys Leu Asn Asp Leu Phe Ala Gln Ser Glu Glu Thr Leu Asn 
            180                 185                 190         


Leu Lys Leu Val Thr Asp Glu Ala Leu Leu Gln Gln Ile Gln Gln Ile 
        195                 200                 205             


Leu Val Arg Thr Asp Leu Ser Arg Ser Glu Gln Gln Arg Gln Ile Trp 
    210                 215                 220                 


Pro Leu Met Ala Val Leu Thr Gly Ala Ser Ala Ala Glu Lys Lys Arg 
225                 230                 235                 240 


Gln Lys Asn Val Val Val Glu Leu Ser Lys Ala Leu Val Gly Leu Lys 
                245                 250                 255     


Ala Lys Met Asn Val Val Thr Leu Thr Glu Val Asp Ala Ala Val Val 
            260                 265                 270         


Lys Asp Trp Thr Phe Thr Met Glu Glu Ser Gln Asp Lys Leu Pro Glu 
        275                 280                 285             


Ile Glu Glu Gln Leu Ser Glu Val Gly Gln Gln Ile Met Asp Glu Val 
    290                 295                 300                 


Ile Gln Leu Tyr Ala Ser Val Asn Leu Ala Gln Leu Ile Pro Ala Gly 
305                 310                 315                 320 


Lys Arg Phe Ser Gln His Met Val Glu Lys Tyr Lys His His Glu Lys 
                325                 330                 335     


Asn Leu Glu Leu Leu Lys Thr Tyr Ile His Ser Gln Ser Asp Ser Lys 
            340                 345                 350         


Arg Gly Arg Glu Ile Arg Ala Thr Tyr Asp Arg Tyr Ile Asp Gly Val 
        355                 360                 365             


Asp Ser Lys Pro Val Thr Gln Glu Met Phe Tyr Lys Asp Leu Met Lys 
    370                 375                 380                 


Tyr Val Glu Ala Asp Ala Thr Ser Asn His Leu Ala Ala Glu Ile Lys 
385                 390                 395                 400 


Asp Glu Ile Asp Ser Glu Gln Phe Met Pro Lys Leu Arg Thr Lys Gln 
                405                 410                 415     


Asn Gly Ser Ile Pro Tyr Gln Val Gln Gln Tyr Glu Leu Asp Gln Ile 
            420                 425                 430         


Ile Ser His Gln Lys Lys Tyr Tyr Pro Trp Leu Gly Glu Glu Asn Pro 
        435                 440                 445             


Val Ala Glu Arg Arg Gly Lys Phe Pro Tyr Lys Leu Asp Glu Leu Val 
    450                 455                 460                 


Gly Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr Lys Glu Asp 
465                 470                 475                 480 


Gln Gln Ala Thr Ser Gly Ala Gly Phe Ala Trp Met Val Arg Lys Ala 
                485                 490                 495     


Asp Gly Pro Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Ile 
            500                 505                 510         


Ala Ser Ala Thr Ala Phe Ile Gln Arg Met Gln Thr Thr Asp Thr Tyr 
        515                 520                 525             


Leu Ile Gly Glu Asp Val Leu Pro Ala Arg Ser Leu Ile Tyr Gln Arg 
    530                 535                 540                 


Phe Met Val Leu Asn Glu Leu Asn Asn Met Arg Val Glu Asp Arg Lys 
545                 550                 555                 560 


Leu Ala Pro Gln Gln Lys Gln Arg Leu Tyr Asn Gln Val Phe Lys Gln 
                565                 570                 575     


His Gln His Val Ser Val Lys Asn Ile Gln Gln Asn Leu Met Asp Ala 
            580                 585                 590         


Gly Glu Tyr Arg Glu Ala Pro Lys Ile Thr Gly Leu Ala Asp Pro Lys 
        595                 600                 605             


Gly Phe Asn Ser Ser Leu Ser Thr Tyr His Asp Phe Lys Lys Ile Leu 
    610                 615                 620                 


Pro Glu Ala Ile Ala Asp Glu His Lys Arg Ala Asp Ile Glu Lys Ile 
625                 630                 635                 640 


Ile Leu Trp Ser Thr Thr Phe Glu Asp Ser Ala Ile Phe Lys Gln Lys 
                645                 650                 655     


Leu Glu Glu Val Ala Trp Leu Thr Asp Ala Gln Arg Lys Gln Leu Ser 
            660                 665                 670         


Gly Leu Arg Tyr Arg Gly Trp Gly Gln Leu Ser His Lys Leu Leu Ile 
        675                 680                 685             


Ala Phe Lys Asp Asp Asn Gly Arg Ser Ile Met Asp Gly Leu Trp Glu 
    690                 695                 700                 


Thr Ser Asp Asn Phe Met Gln Leu Arg Lys Gln Pro Ala Ile Glu Thr 
705                 710                 715                 720 


Gln Ile Lys Glu Ala Asn Gln Ala Asn Leu Thr Asp Ala Asp Ile Gln 
                725                 730                 735     


Asp Thr Ile Asn Glu Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile 
            740                 745                 750         


Arg Glu Val Met Leu Val Leu Asp Asp Ile Lys Asn Ala Met His Gly 
        755                 760                 765             


Gln Thr Pro Ser Trp Ile Phe Val Glu Ala Ala Arg Gly Gly Gly Val 
    770                 775                 780                 


Ala Gly Arg Arg Thr Gln Ser Arg Ser Ser Gln Ile Val Glu Ala Tyr 
785                 790                 795                 800 


Lys Gly Thr Ala Lys Glu Ile Val Ser Glu Lys Val Gln His Glu Leu 
                805                 810                 815     


Asn Glu Lys Ile Lys Ala Lys Ala Asp Phe Asn Thr Arg Leu Val Leu 
            820                 825                 830         


Tyr Phe Leu Gln Asn Gly Arg Asp Leu Tyr Thr Asn Glu Ala Ile Asn 
        835                 840                 845             


Ile Asp Arg Leu Ser Glu Tyr Asp Ile Asp His Ile Leu Pro Gln Ser 
    850                 855                 860                 


Leu Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ala Arg 
865                 870                 875                 880 


Ile Asn Arg Glu Lys Asn Asp Thr Phe Ala Ser Glu Lys Phe Gly Arg 
                885                 890                 895     


Lys Met Gly Ala Gln Trp Arg Glu Leu His Arg Asn Gly Leu Met Thr 
            900                 905                 910         


Gln Arg Lys Leu Lys His Leu Leu Met Arg Pro Asp Glu Ile Ser Lys 
        915                 920                 925             


His Ala Thr Asp Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val 
    930                 935                 940                 


Ile Lys Leu Val Glu Glu Leu Ile Ser Ser Glu Tyr Pro Ala Ala Ser 
945                 950                 955                 960 


Ile Val Ala Val Lys Ala Asn Leu Thr His Gln Phe Arg Gln Thr Phe 
                965                 970                 975     


Asn Phe Pro Lys Leu Arg Glu Val Asn Asp Tyr His His Ala Phe Asp 
            980                 985                 990         


Ala Ser Leu Thr Ala Phe Ile Gly  Met Tyr Leu Leu Lys  Gln Tyr Pro 
        995                 1000                 1005             


Lys Leu  Glu Arg Phe Phe Val  Tyr Gly Lys Phe Ala  Lys Gln Pro 
    1010                 1015                 1020             


Ile Asn  Leu Thr Arg Phe Asn  Ile Ile Arg Lys Leu  Ala Val Ala 
    1025                 1030                 1035             


Glu Lys  Pro Ile Ala Asn Ile  Glu Thr Gly Glu Ile  Leu Trp Asp 
    1040                 1045                 1050             


Lys Thr  Ala Asp Ile Lys Tyr  Phe Glu Lys Leu Tyr  Asn Tyr Lys 
    1055                 1060                 1065             


Arg Leu  Leu Val Thr His Glu  Val Arg Glu Asn Tyr  Gly Ala Met 
    1070                 1075                 1080             


Phe Lys  Gln Thr Leu Phe Lys  Ala Ser Asp Asn Lys  Ser Lys Thr 
    1085                 1090                 1095             


Leu Val  Pro Lys Lys Asn His  Met Glu Thr Ser Val  Tyr Gly Gly 
    1100                 1105                 1110             


Tyr Ser  Asn Gln Glu Thr Ala  Tyr Leu Ala Ile Val  Arg Ile Pro 
    1115                 1120                 1125             


Phe Lys  Ser Gly Phe Lys Phe  Ile Val Val Gly Ile  Pro Thr Arg 
    1130                 1135                 1140             


Met Val  Ala Lys Ile Lys His  Tyr Gln Ser Leu Gly  Ala Thr Leu 
    1145                 1150                 1155             


Lys Gln  Ala Thr His Lys Val  Ile Glu Pro Lys Phe  Thr Lys Ile 
    1160                 1165                 1170             


Ser Arg  Lys Thr Lys Gln Thr  Val Ile Ser Asp Tyr  Glu Val Val 
    1175                 1180                 1185             


Leu Pro  Lys Val Tyr Leu Asp  Gln Val Val Arg Asp  Gln Val Lys 
    1190                 1195                 1200             


Gly Gln  Met Tyr Arg Phe Ser  Leu Gly Ser Asp Lys  Glu Tyr His 
    1205                 1210                 1215             


Asn Val  Gln Glu Leu Tyr Leu  Pro Leu Ser Ile Gln  Gln Ala Phe 
    1220                 1225                 1230             


Val Gly  His Tyr Asp Glu Ser  Asp Asp Gln Arg Ser  Asn Asp Leu 
    1235                 1240                 1245             


Val Lys  Val Tyr Asp Ala Ala  Leu Lys Gln Leu Gln  Arg Tyr Phe 
    1250                 1255                 1260             


Pro Leu  His Leu Ser Arg Asn  Phe Asp Gln Val Ala  Asn Gln Ala 
    1265                 1270                 1275             


His Gln  Ser Phe Glu Gly Leu  Lys Asn Asn Val Gln  Lys Ser Asp 
    1280                 1285                 1290             


Lys Gln  Leu Gly Lys Lys Glu  Val Leu Asn Ser Leu  Phe Val Gly 
    1295                 1300                 1305             


Leu His  Ala Thr Ala Thr Arg  Ser Asn Leu Ser Val  Leu Gly Met 
    1310                 1315                 1320             


Ser Lys  Asp Phe Gly Arg Leu  Gln Ser Arg Ser Gly  Ile Thr Leu 
    1325                 1330                 1335             


Thr Asp  Gln Ala Glu Ile Ile  Tyr Gln Ser Pro Thr  Gly Leu Phe 
    1340                 1345                 1350             


Glu Arg  Lys Val Ala Leu Lys  Asp Leu 
    1355                 1360         


<210>  958
<211>  1362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  958

Met Thr Lys Leu Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg 
            20                  25                  30          


Leu Lys Gly Lys Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys 
        35                  40                  45              


Ala Ala Ala Lys Arg Arg Ala Gly Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Asn Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His 
65                  70                  75                  80  


Ile Thr Pro Thr Asp Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Ala Arg Tyr Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Gln Gln Tyr Ser Thr Met Tyr 
        115                 120                 125             


His Leu Arg Asn Arg Leu Met Thr Asp Pro Ser Arg Ala Asp Val Arg 
    130                 135                 140                 


Glu Ile Tyr Phe Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Asp Ala Lys Asp Phe Asn Thr Asn Asp Val Ala Leu 
                165                 170                 175     


Asp Glu Ile Phe Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Gly Ile Thr Phe Asp Glu Asp Lys Ala Asn Glu Phe Lys Thr 
        195                 200                 205             


Ile Leu Leu Asn Glu Ile Ala Thr Ser Ile Asp Thr Gln Arg Ala Leu 
    210                 215                 220                 


Val Lys Leu Leu Leu Ala Glu Glu Asp Asp Lys Asp Ile Leu Lys Gln 
225                 230                 235                 240 


Gln Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Val Val Gly Leu Lys 
                245                 250                 255     


Thr Lys Leu Asn Leu Ala Leu Gly Thr Glu Val Asp Ser Ser Glu Ala 
            260                 265                 270         


Thr Ala Trp Asn Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp Ala Gly 
        275                 280                 285             


Ile Glu Ser Ala Met Thr Asp Glu Gly Thr Glu Ile Leu Asp Gln Ile 
    290                 295                 300                 


Arg Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Thr Gly 
305                 310                 315                 320 


Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Thr Gln His His Glu 
                325                 330                 335     


Asp Leu Gln Leu Phe Lys Ala Tyr Leu Lys Gln Leu Glu Asp Asp Gly 
            340                 345                 350         


Thr Ala Lys Ala Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp Gly Asp 
        355                 360                 365             


Asp Ala Ala Pro Phe Leu Arg Glu Asn Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Asp Val Thr Ala His Pro Asn Thr Lys Ser Pro Glu Leu Leu Glu Arg 
385                 390                 395                 400 


Leu Ala Gln Pro Asp Phe Met Leu Lys Gln Arg Asn Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Gln Ile Ile Lys 
            420                 425                 430         


Asn Gln Ala Val Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Lys Ser Met Pro Tyr Gln Leu Asp Glu Leu Leu Asn Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Ala Lys Glu Gln Lys 
465                 470                 475                 480 


Ala Ala Gln Gly Gly Val Phe Ala Trp Met Val Arg Lys Asp Pro Glu 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Arg Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asn Glu Lys Leu 
545                 550                 555                 560 


Ser Ile Glu Gln Lys Gln Gln Val Ile Arg Glu Leu Phe Glu Arg His 
                565                 570                 575     


Asn Ser Val Thr Ile Lys Gln Phe Ala Glu Asn Leu Arg Ala His Gly 
            580                 585                 590         


Asp Tyr Ala His Ile Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr Arg Gln Leu Lys Ser Leu Leu Pro 
    610                 615                 620                 


Glu Ala Ile Asp Asp Pro Ala Lys Gln Ala Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Ala Trp Ser Thr Val Phe Glu Asp Ala Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Lys Glu Ile Ser Trp Leu Gly Ser Glu Ala Ile Thr Lys Leu Ser Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Arg Lys Phe Leu Asn Gly 
        675                 680                 685             


Leu Thr Leu Gly Asn Gly His Thr Ile Ile Gln Glu Leu Leu Leu Ser 
    690                 695                 700                 


Thr Asn Asn Leu Met Gln Ile Leu Thr Asp Glu Thr Leu Gln Lys Lys 
705                 710                 715                 720 


Met Thr Glu Leu Asn Ala Asp Lys Leu Lys Thr Ala Asn Ile Asn Asp 
                725                 730                 735     


Ala Ile Asp Asn Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Arg Val Val Asp Asp Ile Lys Arg Ala Ala Asp Gly Gln 
        755                 760                 765             


Asp Pro Ser Trp Leu Tyr Val Glu Thr Ala Asp Gly Gly Gly Thr Pro 
    770                 775                 780                 


Gly Lys Arg Thr Arg Ala Arg Gln His Gln Leu Gln Glu Ile Tyr Ala 
785                 790                 795                 800 


Asn Ala Ala His Glu Leu Ile Asp Thr Ala Val Arg Gly Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Ser Asp Lys Ala Asp Phe Asn Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Ala Pro Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val His Ala Ile Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Ala Thr Phe Ala Ser Thr Ile Tyr Ala Gln Lys 
                885                 890                 895     


Met Asn Ala Thr Trp Arg Gln Trp His Glu Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Arg Asn Leu Gln Met Arg Pro Asp Gln Ile Asp Lys Tyr 
        915                 920                 925             


Ala Ser Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Val Ala Ala Gln Tyr Pro Asp Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu Leu Glu 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Phe Pro Asn 
        995                 1000                 1005             


Leu Gln  Ala Phe Phe Thr Tyr  Gly Lys Phe Thr Lys  Ala Asp Val 
    1010                 1015                 1020             


Lys Lys  Leu Arg Gly Phe Asn  Phe Ile Arg Asp Ile  Thr His Ala 
    1025                 1030                 1035             


Glu Asp  Lys Ile Val Ala Lys  Asp Thr Gly Glu Val  Val Trp Asp 
    1040                 1045                 1050             


Lys Gln  Arg Asp Val Asp Glu  Leu Asp Arg Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val Arg Phe Glu Thr  Ala Asp Leu 
    1070                 1075                 1080             


Phe Lys  Gln Thr Val Tyr Gly  Ala Arg Asp Ser Lys  Glu Ala Gly 
    1085                 1090                 1095             


Gly Ser  Lys Gln Leu Ile Pro  Lys Lys Lys Gly Tyr  Pro Val Asp 
    1100                 1105                 1110             


Ile Tyr  Gly Gly Tyr Phe Arg  Glu Asn Thr Ala Tyr  Leu Ala Val 
    1115                 1120                 1125             


Val Lys  Val Thr Lys Lys Thr  Glu Thr Ile Phe Lys  Val Val Lys 
    1130                 1135                 1140             


Ile Ala  Thr Ser Gln Val Ala  Ala Leu Asn Lys Ala  Arg Ser Arg 
    1145                 1150                 1155             


Ser Thr  Ala Glu Glu Leu Ser  Val Leu Thr Glu Leu  Leu Lys Pro 
    1160                 1165                 1170             


Lys Phe  Ser Lys Val Gly Lys  Asn Gly Lys Ile Thr  Asp Thr Pro 
    1175                 1180                 1185             


Phe Glu  Val Val Leu Pro Arg  Val Pro Arg Glu Gln  Leu Phe Tyr 
    1190                 1195                 1200             


Asn Ala  Lys Tyr Gly Phe Phe  Met Val Asn Ser Asp  Thr Met Phe 
    1205                 1210                 1215             


His Asn  Phe Gln Glu Ile Trp  Val Ser Arg Ser Asp  Gln Lys Ile 
    1220                 1225                 1230             


Leu Gln  Gln Ile Arg Lys Ala  Lys Ile Asp Tyr Pro  Asn Val Asp 
    1235                 1240                 1245             


Gln Asp  Leu Asp Asn Leu Phe  His Asn Leu Ala Asp  Gln Ile Val 
    1250                 1255                 1260             


Lys Tyr  Phe Asp Leu Tyr Ser  Ile Ala Gly Phe Lys  Glu Lys Ile 
    1265                 1270                 1275             


Ser Gln  Ser Gln Asn Thr Phe  Asn Asp Leu Ala Val  Asp Asp Thr 
    1280                 1285                 1290             


Asp Gln  Ser Val Gly Lys Ile  Thr Val Ile Asn Glu  Leu Leu Lys 
    1295                 1300                 1305             


Gly Ala  Gln Ala Asn Gly Met  Thr Gly Ser Leu Lys  Val Leu Lys 
    1310                 1315                 1320             


Ile Ser  Thr Pro Phe Gly Phe  Thr Gln Asp Lys Ser  Gly Val Leu 
    1325                 1330                 1335             


Thr Lys  Asp Ser Ser Ile Ile  Tyr Gln Ser Pro Thr  Gly Leu Phe 
    1340                 1345                 1350             


Glu Arg  Ser Val Arg Leu Thr  Asp Leu 
    1355                 1360         


<210>  959
<211>  1362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  959

Met Thr Lys Leu Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg 
            20                  25                  30          


Leu Lys Gly Lys Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys 
        35                  40                  45              


Ala Ala Ala Lys Arg Arg Ala Gly Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Asn Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His 
65                  70                  75                  80  


Ile Thr Pro Thr Asp Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Ala Arg Tyr Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Gln Gln Tyr Ser Thr Met Tyr 
        115                 120                 125             


His Leu Arg Asn Arg Leu Met Thr Asp Pro Ser Arg Ala Asp Val Arg 
    130                 135                 140                 


Glu Ile Tyr Phe Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Asp Ala Lys Asp Phe Asn Thr Asn Asp Val Ala Leu 
                165                 170                 175     


Asp Glu Ile Phe Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Gly Ile Thr Phe Asp Glu Asp Lys Glu Asn Glu Phe Lys Thr 
        195                 200                 205             


Ile Leu Leu Asn Glu Ile Ala Thr Ser Ile Asp Thr Gln Arg Ala Leu 
    210                 215                 220                 


Val Lys Leu Leu Leu Ala Glu Glu Asp Asp Lys Asp Ile Leu Lys Gln 
225                 230                 235                 240 


Gln Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Val Val Gly Leu Lys 
                245                 250                 255     


Thr Lys Leu Asn Leu Ala Leu Gly Thr Glu Val Asp Ser Ser Glu Ala 
            260                 265                 270         


Thr Ala Trp Asn Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp Ala Gly 
        275                 280                 285             


Ile Glu Ser Ala Met Thr Asp Glu Gly Thr Glu Ile Leu Asp Gln Ile 
    290                 295                 300                 


Arg Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Thr Gly 
305                 310                 315                 320 


Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Thr Gln His His Glu 
                325                 330                 335     


Asp Leu Gln Leu Phe Lys Ala Tyr Leu Lys Gln Leu Glu Asp Asp Gly 
            340                 345                 350         


Thr Ala Lys Ala Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp Gly Asp 
        355                 360                 365             


Asp Ala Ala Pro Phe Leu Arg Glu Asn Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Asp Val Thr Ala His Pro Asn Thr Lys Ser Pro Glu Leu Leu Glu Arg 
385                 390                 395                 400 


Leu Ala Gln Pro Asp Phe Met Leu Lys Gln Arg Asn Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Gln Ile Ile Lys 
            420                 425                 430         


Asn Gln Ala Val Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Lys Ser Met Pro Tyr Gln Leu Asp Glu Leu Leu Asn Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Ala Lys Glu Gln Lys 
465                 470                 475                 480 


Ala Ala Gln Gly Gly Val Phe Ala Trp Met Val Arg Lys Asp Pro Glu 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Arg Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asn Glu Lys Leu 
545                 550                 555                 560 


Ser Ile Glu Gln Lys Gln Gln Val Ile Arg Glu Leu Phe Glu Arg His 
                565                 570                 575     


Asn Ser Val Thr Ile Lys Gln Phe Ala Glu Asn Leu Arg Ala His Gly 
            580                 585                 590         


Asp Tyr Ala His Ile Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr Arg Gln Leu Lys Ser Leu Leu Pro 
    610                 615                 620                 


Glu Ala Ile Asp Asp Pro Ala Lys Gln Ala Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Ala Trp Ser Thr Val Phe Glu Asp Ala Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Lys Glu Ile Ser Trp Leu Gly Ser Glu Ala Ile Thr Lys Leu Ser Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Arg Lys Phe Leu Asn Gly 
        675                 680                 685             


Leu Thr Leu Gly Asn Gly His Thr Ile Ile Gln Glu Leu Leu Leu Ser 
    690                 695                 700                 


Thr Asn Asn Leu Met Gln Ile Leu Thr Asp Glu Thr Leu Gln Lys Lys 
705                 710                 715                 720 


Met Thr Glu Leu Asn Ala Asp Lys Leu Lys Thr Ala Asn Ile Asn Asp 
                725                 730                 735     


Ala Ile Asp Asn Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Arg Val Val Asp Asp Ile Lys Arg Ala Ala Asp Gly Gln 
        755                 760                 765             


Asp Pro Ser Trp Leu Tyr Val Glu Thr Ala Asp Gly Gly Gly Thr Pro 
    770                 775                 780                 


Gly Lys Arg Thr Arg Ala Arg Gln His Gln Leu Gln Glu Ile Tyr Ala 
785                 790                 795                 800 


Asn Ala Ala His Glu Leu Ile Asp Thr Ala Val Arg Gly Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Ser Asp Lys Ala Asp Phe Asn Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Ala Pro Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val His Ala Ile Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Ala Thr Phe Ala Ser Thr Ile Tyr Ala Gln Lys 
                885                 890                 895     


Met Asn Ala Thr Trp Arg Gln Trp His Glu Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Arg Asn Leu Gln Met Arg Pro Asp Gln Ile Asp Lys Tyr 
        915                 920                 925             


Ala Ser Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Val Ala Ala Gln Tyr Pro Asp Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu Leu Glu 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Phe Pro Asn 
        995                 1000                 1005             


Leu Gln  Ala Phe Phe Thr Tyr  Gly Lys Phe Thr Lys  Ala Asp Val 
    1010                 1015                 1020             


Lys Lys  Leu Arg Gly Phe Asn  Phe Ile Arg Asp Ile  Thr His Ala 
    1025                 1030                 1035             


Glu Asp  Lys Ile Val Ala Lys  Asp Thr Gly Glu Val  Val Trp Asp 
    1040                 1045                 1050             


Lys Gln  Arg Asp Val Asp Glu  Leu Asp Arg Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val Arg Phe Glu Thr  Ala Asp Leu 
    1070                 1075                 1080             


Phe Lys  Gln Thr Val Tyr Gly  Ala Arg Asp Ser Lys  Glu Ala Gly 
    1085                 1090                 1095             


Gly Ser  Lys Gln Leu Ile Pro  Lys Lys Lys Gly Tyr  Pro Val Asp 
    1100                 1105                 1110             


Ile Tyr  Gly Gly Tyr Phe Arg  Glu Asn Thr Ala Tyr  Leu Ala Val 
    1115                 1120                 1125             


Val Lys  Val Thr Lys Lys Thr  Glu Thr Ile Phe Lys  Val Val Lys 
    1130                 1135                 1140             


Ile Ala  Thr Ser Gln Val Ala  Ala Leu Asn Lys Ala  Arg Ser Arg 
    1145                 1150                 1155             


Ser Thr  Ala Glu Glu Leu Ser  Val Leu Thr Glu Leu  Leu Lys Pro 
    1160                 1165                 1170             


Lys Phe  Ser Lys Val Gly Lys  Asn Gly Lys Ile Thr  Asp Thr Pro 
    1175                 1180                 1185             


Phe Glu  Val Val Leu Pro Arg  Val Pro Arg Glu Gln  Leu Phe Tyr 
    1190                 1195                 1200             


Asn Ala  Lys Tyr Gly Phe Phe  Met Val Asn Ser Asp  Thr Met Phe 
    1205                 1210                 1215             


His Asn  Phe Gln Glu Ile Trp  Val Ser Arg Ser Asp  Gln Lys Ile 
    1220                 1225                 1230             


Leu Gln  Gln Ile Arg Lys Ala  Lys Ile Asp Tyr Pro  Asn Val Asp 
    1235                 1240                 1245             


Gln Asp  Leu Asp Asn Leu Phe  His Asn Leu Ala Asp  Gln Ile Val 
    1250                 1255                 1260             


Lys Tyr  Phe Asp Leu Tyr Ser  Ile Ala Gly Phe Lys  Glu Lys Ile 
    1265                 1270                 1275             


Ser Gln  Ser Gln Asn Thr Phe  Asn Asp Leu Ala Val  Asp Asp Thr 
    1280                 1285                 1290             


Asp Gln  Ser Val Gly Lys Ile  Thr Val Ile Asn Glu  Leu Leu Lys 
    1295                 1300                 1305             


Gly Ala  Gln Ala Asn Gly Met  Thr Gly Ser Leu Lys  Val Leu Lys 
    1310                 1315                 1320             


Ile Ser  Thr Pro Phe Gly Phe  Thr Gln Asp Lys Ser  Gly Val Leu 
    1325                 1330                 1335             


Thr Lys  Asp Ser Ser Ile Ile  Tyr Gln Ser Pro Thr  Gly Leu Phe 
    1340                 1345                 1350             


Glu Arg  Ser Val Arg Leu Thr  Asp Leu 
    1355                 1360         


<210>  960
<211>  1362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  960

Met Thr Lys Leu Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg 
            20                  25                  30          


Leu Lys Gly Lys Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys 
        35                  40                  45              


Ala Ala Ala Asp Arg Arg Ala Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Asn Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His 
65                  70                  75                  80  


Ile Thr Pro Thr Asp Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Ser Arg Tyr Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Lys Tyr Glu Asn Cys Pro Thr 
        115                 120                 125             


Met Tyr His Leu Arg Asn Arg Leu Met Thr Asp Pro Ser Lys Ala Asp 
    130                 135                 140                 


Val Arg Glu Ile Tyr Phe Ala Ile His His Ile Leu Lys Ser Arg Gly 
145                 150                 155                 160 


His Phe Leu Thr Pro Gly Asp Ala Lys Asp Phe Asn Thr Asn Lys Val 
                165                 170                 175     


Ala Val Asn Lys Ile Phe Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val 
            180                 185                 190         


Tyr Pro Asp Leu Asp Ile Thr Phe Asp Glu Asn Lys Val Asn Glu Phe 
        195                 200                 205             


Lys Thr Val Leu Leu Asn Glu Lys Ala Thr Pro Ser Asp Thr Gln Arg 
    210                 215                 220                 


Ala Leu Val Asn Leu Leu Leu Ala Lys Glu Gly Asp Arg Asp Ile Leu 
225                 230                 235                 240 


Lys Gln Gln Lys Gln Ala Leu Thr Glu Phe Ala Lys Ala Val Val Gly 
                245                 250                 255     


Leu Lys Thr Lys Leu Asn Val Ala Leu Gly Thr Glu Val Asp Ser Ser 
            260                 265                 270         


Glu Ala Thr Ala Trp Asn Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp 
        275                 280                 285             


Thr Gly Ile Glu Ser Ala Met Thr Asp Glu Gly Thr Glu Ile Leu Asp 
    290                 295                 300                 


Gln Ile Arg Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro 
305                 310                 315                 320 


Ala Gly Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Ala Gln His 
                325                 330                 335     


His Glu Asp Leu Glu Leu Phe Lys Asp Tyr Leu Lys Gln Leu Glu Asp 
            340                 345                 350         


Asp Gly Thr Ala Lys Ala Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp 
        355                 360                 365             


Gly Asp Asp Ala Lys Pro Phe Val Arg Glu Asp Phe Val Lys Ala Leu 
    370                 375                 380                 


Thr Lys Glu Val Thr Ala His Pro Asn Ala Lys Ser Pro Glu Leu Leu 
385                 390                 395                 400 


Glu Arg Leu Ala Gln Pro Asp Phe Met Leu Lys Gln Arg Asn Lys Ala 
                405                 410                 415     


Asn Gly Ala Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Gln Ile 
            420                 425                 430         


Ile Lys Asn Gln Ser Val Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro 
        435                 440                 445             


Val Glu Lys His Arg Lys Ser Met Pro Tyr Gln Leu Asp Glu Leu Leu 
    450                 455                 460                 


Asn Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Ala Lys Glu 
465                 470                 475                 480 


Gln Lys Ala Ala Gln Gly Gly Val Phe Ala Trp Met Val Arg Lys Asp 
                485                 490                 495     


Pro Asp Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg 
            500                 505                 510         


Glu Ala Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr 
        515                 520                 525             


Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln 
    530                 535                 540                 


Ser Tyr Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asp Asn 
545                 550                 555                 560 


Lys Leu Ser Phe Asp Gln Lys Gln Gln Val Leu Gly Glu Leu Phe Glu 
                565                 570                 575     


Arg His Asn Thr Val Thr Ile Lys Gln Phe Val Asp Asn Leu Leu Ala 
            580                 585                 590         


His Gly Asp Tyr Asp Arg Ile Pro Glu Val Arg Gly Leu Ala Asp Glu 
        595                 600                 605             


Lys Arg Phe Leu Ser Ser Leu Ser Thr Tyr Asn Gln Leu Lys Gly Ile 
    610                 615                 620                 


Leu His Glu Ala Ile Asp Asp Ser Asp Lys Gln Thr Asp Ile Glu Asn 
625                 630                 635                 640 


Ile Ile Ala Trp Ser Thr Ile Phe Glu Asp Lys Ala Ile Phe Lys Thr 
                645                 650                 655     


Lys Leu Asn Glu Ile Ser Trp Leu Asn Ala Gly Met Ile Asn Ser Leu 
            660                 665                 670         


Ala Asn Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser His Lys Leu Leu 
        675                 680                 685             


Asn Glu Leu Thr Leu Ser Thr Gly Arg Thr Val Phe Glu Glu Leu Leu 
    690                 695                 700                 


Ile Ser Ser His Asn Leu Met Gln Ile Leu Thr Asp Glu Val Leu Lys 
705                 710                 715                 720 


Gln Lys Ile Ala Glu Leu Asn Ala His Glu Leu Lys Ala Ser Gly Ile 
                725                 730                 735     


Tyr Ala Ala Ile Asp Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala 
            740                 745                 750         


Leu Arg Gln Val Leu Ser Val Ile Asp Asp Ile Thr Asn Ala Ala Gly 
        755                 760                 765             


Gln Ala Pro Asn Trp Leu Tyr Ile Glu Thr Ala Asp Gly Asp Gly Val 
    770                 775                 780                 


Pro Gly Lys Arg Thr Asn Ser Arg Gln Arg Lys Ile Gln Glu Ile Tyr 
785                 790                 795                 800 


Ala Asn Ala Ala Gln Glu Leu Ile Thr Gly Ser Val Arg Gly Glu Leu 
                805                 810                 815     


Glu Asp Lys Ile Ala Ser Arg Ala Asp Phe Thr Asp Arg Leu Val Leu 
            820                 825                 830         


Tyr Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Lys Pro Leu Asn 
        835                 840                 845             


Ile Asp Asn Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser 
    850                 855                 860                 


Leu Ile Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val His Pro Leu 
865                 870                 875                 880 


Val Asn Arg Glu Lys Asp Thr Ile Phe Ala Ser Thr Arg Phe Ala Lys 
                885                 890                 895     


Gln Met Asn Gly Met Trp Arg Gln Trp His Ala Ala Gly Leu Ile Ser 
            900                 905                 910         


Ser Arg Lys Leu Arg Asn Leu Gln Met Arg Pro Asp Glu Ile Asp Lys 
        915                 920                 925             


Tyr Ala Ser Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile 
    930                 935                 940                 


Ile Lys Leu Thr Glu Gln Ile Val Ala Asp Gln Tyr Pro Asp Thr Lys 
945                 950                 955                 960 


Ile Ile Ala Val Lys Ala Gly Leu Ser Ser Gln Leu Arg Lys Glu Leu 
                965                 970                 975     


Asp Phe Pro Lys Asn Arg Glu Val Asn His Tyr His His Ala Phe Asp 
            980                 985                 990         


Ala Phe Leu Ala Ala Arg Ile Gly  Thr Tyr Leu Leu Lys  Arg Tyr Pro 
        995                 1000                 1005             


Asn Leu  Glu Pro Phe Phe Thr  Tyr Gly Lys Phe Lys  Lys Thr Glu 
    1010                 1015                 1020             


Val Lys  Lys Leu Lys Ser Phe  Asn Phe Ile Arg Asp  Met Thr His 
    1025                 1030                 1035             


Ala Lys  Asp Lys Ile Val Ala  Lys Glu Thr Gly Glu  Ile Val Trp 
    1040                 1045                 1050             


Asp Asn  Ala Ser Asp Ile Asn  Glu Leu Asp Arg Ile  Tyr Asn Phe 
    1055                 1060                 1065             


Lys Arg  Met Leu Ile Thr His  Glu Val Arg Phe Glu  Thr Ala Ser 
    1070                 1075                 1080             


Leu Phe  Lys Gln Thr Leu Tyr  Ala Ala Lys Asn Ser  Lys Asn Arg 
    1085                 1090                 1095             


Gly Gly  Ser Arg Gln Leu Ile  Pro Lys Lys Lys Gly  Tyr Leu Val 
    1100                 1105                 1110             


Asp Ile  Tyr Gly Gly Tyr Thr  Gln Glu Thr Gly Ser  Tyr Leu Ser 
    1115                 1120                 1125             


Val Val  Arg Leu Thr Lys Lys  Ala Met Tyr Ala Val  Val Lys Val 
    1130                 1135                 1140             


Ser Thr  Arg Asp Ala Ala Lys  Leu Ala Val Ala Lys  Ser Ile Ser 
    1145                 1150                 1155             


Glu Gln  Lys Glu Asn Glu Thr  Leu Lys Lys Ile Ile  Asp Gly Lys 
    1160                 1165                 1170             


Leu Ser  Lys Thr Ser Lys Lys  Gly Lys Thr Thr His  Gln Leu Phe 
    1175                 1180                 1185             


Glu Ile  Val Leu Pro Arg Val  Gly Gln Lys Thr Leu  Phe Lys Asn 
    1190                 1195                 1200             


Ser Lys  Tyr Asn Gln Phe Leu  Val Asn Ser Asp Thr  Tyr Met His 
    1205                 1210                 1215             


Asn Tyr  Gln Glu Leu Trp Met  Pro Arg Glu Tyr Gln  Arg Met Trp 
    1220                 1225                 1230             


Lys Asp  Ile Leu Leu Ser Asn  His Gly Asp Ala Gln  Leu Glu Gly 
    1235                 1240                 1245             


Gln Leu  Asp Gln Ile Phe Lys  Phe Ile Val Ser Gln  Val Asn Ser 
    1250                 1255                 1260             


Tyr Phe  Asn Leu Tyr Asp Ile  Asn Gln Phe Arg Lys  Lys Ile Ala 
    1265                 1270                 1275             


Asp Ala  Thr Asp Lys Phe Ala  Lys Leu Pro Thr His  Asp Thr Asp 
    1280                 1285                 1290             


Asn Ala  Arg Gly Lys Ile Ala  Thr Ile Gly Gln Leu  Leu Ile Gly 
    1295                 1300                 1305             


Leu Gln  Ala Asn Ala Met Arg  Ser Asp Leu Arg Asn  Leu Asp Ile 
    1310                 1315                 1320             


Lys Thr  Pro Phe Gly Leu Leu  Gln Ile Gly Thr Gly  Ile Thr Leu 
    1325                 1330                 1335             


Asp Leu  Asp Thr Ser Ile Ile  Tyr Gln Ser Pro Thr  Gly Leu Phe 
    1340                 1345                 1350             


Glu Arg  Glu Val Pro Leu Thr  Asp Leu 
    1355                 1360         


<210>  961
<211>  1362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  961

Met Asn Gln Glu Pro Tyr Ala Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Leu Gly Trp Ala Asn Ala Asp Met Asp Gly Tyr Ile Ala Arg Val Lys 
            20                  25                  30          


Gly Gln Thr Gly Leu Gly Val Arg Leu Phe Glu Glu Gly Gln Pro Ala 
        35                  40                  45              


Ala Asp Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Arg Trp Arg Leu Arg Leu Leu Arg Glu Ile Phe Asp Glu Pro Ile Ser 
65                  70                  75                  80  


Ser Ile Asp Pro Asn Phe Phe Ala Arg Gln Lys Ala Ser Ser Ile Ser 
                85                  90                  95      


Pro Lys Asp Asp Arg Phe Glu Thr Thr Tyr Gln Leu Phe Asn Asp Lys 
            100                 105                 110         


Thr Asp Gln Glu Phe Tyr Ser Lys Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Trp Ala Leu Met Thr Glu His Arg Lys Phe Asp Leu Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Val His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Arg Ser 
145                 150                 155                 160 


Gly Asn Pro Lys Ser Phe Lys Pro Ser Glu Leu Asp Phe Ser Gln Arg 
                165                 170                 175     


Phe Lys Glu Val Asn Gly Leu Trp His Leu Ile Phe Asp Glu Val Ala 
            180                 185                 190         


Pro Gln Leu Pro Thr Asp Asn Leu Glu Glu Ile Thr Lys Leu Val Leu 
        195                 200                 205             


Asp Thr Thr Arg Thr Arg Ala Asp Arg Gln Arg Glu Ser Val Lys Leu 
    210                 215                 220                 


Leu Met Ala Ala Thr Asp Asn His Lys Asp Trp Lys Pro Tyr Leu Thr 
225                 230                 235                 240 


Glu Phe Thr Lys Ala Ile Leu Gly Leu Lys Thr Lys Leu Tyr Leu Leu 
                245                 250                 255     


Thr Gly Val Asp Val Ala Lys Ala Asp Gln Lys Thr Trp Thr Phe Ser 
            260                 265                 270         


Leu Glu Asp Ile Glu Asp His Arg Ala Asp Leu Glu Thr Ala Phe Asp 
        275                 280                 285             


Asp Arg Ser Leu Glu Leu Ile Glu Lys Leu Val Asp Leu Gln Ala Ala 
    290                 295                 300                 


Ile Gln Leu Thr Glu Ile Met Pro Ser Gly Gln Tyr Phe Ser Gln Ser 
305                 310                 315                 320 


Met Ile Tyr Arg Tyr Asp Gln His Lys Glu His Leu Arg Trp Leu Lys 
                325                 330                 335     


Asp Tyr Ala Glu Gln Gln Thr Asp Ala Asn Lys Arg Lys Ala Val Arg 
            340                 345                 350         


Leu Ala Tyr Asp His Tyr Ile Glu Gly Asp Ala Lys Gly Lys Pro Glu 
        355                 360                 365             


Ile Ala Asp Asp Phe Tyr Lys Glu Leu Asn Arg Ile Leu Lys Gly Asp 
    370                 375                 380                 


Thr Ser Glu Leu Ala Lys Lys Ile Asn His Glu Ile Ala Leu Glu His 
385                 390                 395                 400 


Phe Met Pro Lys Gln Arg Thr Lys Glu Asn Gly Val Ile Pro Tyr Gln 
                405                 410                 415     


Ile His Gln Gln Glu Leu Asp Ser Ile Ile Asp Asn Gln Lys Lys Tyr 
            420                 425                 430         


Tyr Gln Phe Leu Gly Glu Asp Asn Pro Val Val Ser His His Gly Ser 
        435                 440                 445             


Gln Pro Tyr Lys Ile Asp Glu Leu Val Ser Phe Arg Val Pro Tyr Tyr 
    450                 455                 460                 


Val Gly Pro Met Ile Thr Ala Asp Gln Gln Lys Lys Thr Ala Ala Gly 
465                 470                 475                 480 


Gln Phe Ala Trp Met Val Arg Lys Asp Ser Gly Asp Ile Thr Pro Trp 
                485                 490                 495     


Asn Phe Asp Asp Lys Val Asp Arg Thr Ala Ser Ala Thr Asn Phe Ile 
            500                 505                 510         


Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu Ile Gly Glu Asp Val Leu 
        515                 520                 525             


Pro Leu Gln Ser Leu Thr Tyr Gln Lys Phe Met Val Leu Asn Glu Leu 
    530                 535                 540                 


Asn Asn Ile Arg Ile Asn Gly Glu Leu Leu Thr Arg Glu Gln Lys Gln 
545                 550                 555                 560 


Arg Thr Tyr Asn Asp Val Phe Lys Gly His Gly Thr Val Asn Val Lys 
                565                 570                 575     


Ser Leu Ile Lys Ser Leu Glu Cys His Ser Glu Val Thr Gly Ala Ile 
            580                 585                 590         


Lys Val Glu Gly Leu Ala Asp Pro Lys Arg Phe Asn Ser Ala Leu Thr 
        595                 600                 605             


Thr Tyr Arg Asp Phe Lys Lys Ile Leu Pro Val Ala Ile Asp Gln Pro 
    610                 615                 620                 


Gln Arg Gln Ala Asp Ile Glu Arg Ile Ile Asn Trp Ser Thr Ile Phe 
625                 630                 635                 640 


Glu Asp Ala Asp Ile Phe Lys Ala Lys Leu Gln Glu Ile Asp Trp Leu 
                645                 650                 655     


Thr Asp Thr Gln Arg Asp Arg Leu Ser Thr Lys Arg Tyr Arg Gly Trp 
            660                 665                 670         


Gly Arg Leu Ser Ala Lys Leu Leu Leu Asn Ile Lys Asp Thr Gln Gly 
        675                 680                 685             


Leu Ser Ile Met Asp Gln Leu Trp Asn Thr Gln His Asn Phe Met Gln 
    690                 695                 700                 


Ile Val His Glu Pro Asp Tyr Ala Glu Lys Ile Glu Lys Thr Asn Ala 
705                 710                 715                 720 


Lys Gly Leu Lys Gln Gln Asp Ile Phe Glu Thr Ile Asn Glu Met Tyr 
                725                 730                 735     


Thr Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Ile Leu Ala Val Val 
            740                 745                 750         


Arg Asp Ile Gln Lys Ala His His Gly Gln Ala Pro Ser Arg Ile Phe 
        755                 760                 765             


Ile Glu Ala Ala Arg Gly Ala Glu Gln Asn Pro Gln Arg Thr Arg Gln 
    770                 775                 780                 


Arg Gln Lys Gln Leu Ala Asp Leu Phe Ala Asp Gln Ala Lys Glu Ile 
785                 790                 795                 800 


Val Asn Glu Thr Val Ala Gln Glu Leu Asn Asp Glu Thr Asp Asn Lys 
                805                 810                 815     


Ala Arg Phe Thr Asp Arg Leu Met Leu Tyr Phe Leu Gln Asn Gly Gln 
            820                 825                 830         


Asp Met Tyr Thr Gly Glu Lys Leu Asn Ile Asp Lys Leu Ser Thr Tyr 
        835                 840                 845             


Asp Ile Asp His Ile Leu Pro Gln Ser Leu Val Lys Asp Asp Ser Leu 
    850                 855                 860                 


Asp Asn Arg Val Leu Val Ser Lys Val Glu Asn Leu Lys Lys Asn Asp 
865                 870                 875                 880 


His Phe Ala Ser Glu Leu Tyr Ala Arg Lys Met Gln Gly Thr Trp Glu 
                885                 890                 895     


Leu Trp Arg Ser Ala Gly Leu Ile Ser Ala Arg Lys Leu Lys His Leu 
            900                 905                 910         


Leu Met Arg Pro Asp Glu Ile Asn Lys Tyr Ala Thr Gly Phe Ile Arg 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Arg Gln Val Ile Lys Leu Val Thr Asp Val 
    930                 935                 940                 


Leu Ser Asn Glu Tyr Asp Ser Asp Lys Thr Lys Ile Ile Ser Val Lys 
945                 950                 955                 960 


Ala Gly Leu Ser His Gln Leu Arg Met Glu Leu Asp Leu Pro Lys Val 
                965                 970                 975     


Arg Glu Leu Asn Asp Tyr His His Ala Phe Asp Ala Phe Leu Ala Val 
            980                 985                 990         


Arg Ile Gly Thr Tyr Leu Leu Lys  Arg Tyr Pro Lys Leu  Glu Arg Phe 
        995                 1000                 1005             


Phe Val  Tyr Gly Gln Tyr Gln  Val Ala Pro Gln Leu  Asp Leu Arg 
    1010                 1015                 1020             


Arg Phe  Asn Phe Ile His Asp  Leu Val Ser Gly Lys  Gln Glu Lys 
    1025                 1030                 1035             


Ile Val  Ala Glu Glu Thr Gly  Glu Ile Val Trp Asn  Arg Glu Lys 
    1040                 1045                 1050             


Asp Val  Ser Tyr Met Lys His  Leu Leu Ser Leu Lys  His Leu Leu 
    1055                 1060                 1065             


Val Thr  His Glu Val Lys Glu  Asp His Gly Ala Leu  Phe Asn Gln 
    1070                 1075                 1080             


Thr Ile  Tyr Lys Ala Lys Lys  Asp Asn Gly Asn Val  Gly Gly Lys 
    1085                 1090                 1095             


Met Lys  Leu Leu Pro Thr Lys  Ser Asn Arg Pro Thr  Glu Ile Tyr 
    1100                 1105                 1110             


Gly Gly  His Ser Ser Gln Ala  Ile Ala Tyr Leu Ala  Ile Val Arg 
    1115                 1120                 1125             


Val Asn  Gly Lys Glu Pro Ser  Tyr Arg Val Met Gly  Val Pro Ile 
    1130                 1135                 1140             


Arg Ile  Leu Asp Gln Leu Lys  Gln Ala Lys Lys Gln  Gly Glu Tyr 
    1145                 1150                 1155             


Ala Glu  Arg Leu Ala Leu Lys  Gln Phe Phe Thr Pro  Asp Phe Thr 
    1160                 1165                 1170             


Lys Gly  Lys Gly Lys Ile Val  Ser Asp Phe Asp Val  Val Ile Pro 
    1175                 1180                 1185             


His Ile  Pro Leu Leu Gln Pro  Val Ile Asp Glu Ile  Ser Gly Gln 
    1190                 1195                 1200             


Ser His  Arg Phe Ala Leu Gly  Thr Asn Thr Tyr Tyr  Arg Asn Leu 
    1205                 1210                 1215             


Gln Gln  Leu Ile Leu Thr Leu  Ala Asp Gln Lys Val  Leu Ile Ser 
    1220                 1225                 1230             


Lys Glu  Thr Gly Val Asp Glu  Glu Lys Lys Leu Val  Glu Val Tyr 
    1235                 1240                 1245             


Ser His  Ile Cys Gln Gln Val  Met Lys Phe Phe Pro  Leu Tyr Asp 
    1250                 1255                 1260             


Arg Asn  Lys Phe Arg Glu Leu  Leu Val Asp Ser Arg  Ser His Phe 
    1265                 1270                 1275             


Glu Gln  Leu Pro Ile Val Asn  Arg Tyr Ser Asp Ala  Gly Lys Leu 
    1280                 1285                 1290             


Glu Ile  Ala Gly Lys Arg Gly  Ile Leu Gln Gln Ile  Met Val Gly 
    1295                 1300                 1305             


Leu His  Ala Asn Ala Thr Pro  Gly Asp Leu Lys Glu  Leu Gly Val 
    1310                 1315                 1320             


Lys Thr  Pro Phe Gly Lys Leu  Gln Val Pro Gly Gly  Ile Lys Leu 
    1325                 1330                 1335             


Thr Glu  Asn Ala Lys Leu Ile  Tyr Glu Ser Pro Thr  Gly Leu Phe 
    1340                 1345                 1350             


Lys Arg  Glu Val Lys Ile Thr  Thr Glu 
    1355                 1360         


<210>  962
<211>  1362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  962

Met Lys Lys Lys Ala Glu Lys Ile Tyr Leu Gly Leu Asn Val Gly Thr 
1               5                   10                  15      


Asn Ser Ile Gly Tyr Ala Val Thr Asp Glu Gln Tyr Arg Leu Leu Lys 
            20                  25                  30          


Phe Asn Gly Asn Asp Ala Trp Gly Ser Ile Val Phe Asp Ala Ala Ser 
        35                  40                  45              


Ser Asn Glu Asp Arg Arg Asn His Arg Leu Ala Arg Arg Asn Leu Asp 
    50                  55                  60                  


Arg Arg Gln Gln Arg Ile Val Trp Leu Gln Glu Ile Phe Ala Lys Glu 
65                  70                  75                  80  


Ile Ser Lys Val Asp Asp Arg Phe Phe Ile Arg Leu Ser Glu Ser Phe 
                85                  90                  95      


Arg Trp Arg Glu Asp Val Gln Asp Arg Tyr Val Phe Phe Asn Asp Lys 
            100                 105                 110         


Glu Tyr Thr Asp Val Gln Tyr Met Lys Glu Tyr Pro Thr Ile His His 
        115                 120                 125             


Leu Ile Cys Glu Leu Met Asp Asn Lys Thr Pro His Asp Val Arg Leu 
    130                 135                 140                 


Val Tyr Leu Ala Cys Ala Trp Leu Ile Thr His Arg Gly His Phe Leu 
145                 150                 155                 160 


Asn Asn Leu Asn Val Glu Lys Leu Asp Glu Ile Met Asp Phe Ser Ser 
                165                 170                 175     


Val Tyr Gln Asn Phe Ile Asn Tyr Phe Thr Glu Asn Gly Tyr Lys Arg 
            180                 185                 190         


Pro Trp Gly Glu Ile Asp Val Ser Ala Leu Ser Glu Val Leu Lys Gln 
        195                 200                 205             


Lys Thr Gly Ile Thr Val Lys Asn Lys Glu Leu Gln Asn Ile Leu Leu 
    210                 215                 220                 


Glu Gly Lys Lys Pro Glu Lys Ser Gly Thr Glu Glu Phe Pro Phe Ser 
225                 230                 235                 240 


Gln Asp Ser Ile Ile Arg Leu Leu Ala Gly Gly Gln Cys Lys Leu Lys 
                245                 250                 255     


Asp Val Phe Cys Lys Glu Glu Tyr Leu Asp Leu Gly Ser Val Ser Leu 
            260                 265                 270         


Gly Met Asp Glu Glu Lys Phe Asp Glu Ile Ser Gly Ser Ile Gly Glu 
        275                 280                 285             


Asp Tyr Asp Leu Leu Ile Ala Leu Arg Gly Leu Tyr Asp Trp Ser Val 
    290                 295                 300                 


Leu Ala Asn Leu Leu Asn Gly His Ser Glu Thr Ile Ser Met Ala Lys 
305                 310                 315                 320 


Val Gln Met Tyr Glu Gln His Gly Lys Asp Leu Gln Ile Leu Lys Tyr 
                325                 330                 335     


Phe Val Arg Lys Tyr Val Pro Asp Lys Tyr Asn Glu Val Phe Arg Lys 
            340                 345                 350         


Ala Lys Ala Asp Asn Tyr Val Ala Tyr Thr Gly His Met Asp Lys Asp 
        355                 360                 365             


Thr Ala Ser Gln Ile Lys Lys Lys Ala Lys Ala Glu Glu Phe Ser Lys 
    370                 375                 380                 


Tyr Val Leu Lys Leu Met Ser Pro Ile Lys Pro Glu Lys Thr Asp Leu 
385                 390                 395                 400 


Gln Ser Tyr Glu Asp Met Cys Glu Arg Leu Lys Leu Asn Arg Phe Leu 
                405                 410                 415     


Pro Lys Gln Lys Asn Thr Asp Asn Arg Val Ile Pro His Gln Leu Tyr 
            420                 425                 430         


Glu Tyr Glu Leu Ile Gln Ile Leu Lys Asn Ala Ser Val Tyr Leu Pro 
        435                 440                 445             


Phe Leu Asn Asp Thr Asn Asn Gly Ile Ser Glu Ala Asp Lys Val Leu 
    450                 455                 460                 


Ser Val Phe Arg Tyr Lys Ile Pro Tyr Tyr Val Gly Pro Leu Asn Glu 
465                 470                 475                 480 


Glu Ser Ser Phe Ala Trp Ile Ser Arg Lys Gln Gly Lys Ile Thr Pro 
                485                 490                 495     


Trp Asn Phe Lys Glu Met Val Asp Glu Asp Ala Ser Glu Asn Ala Phe 
            500                 505                 510         


Ile Arg Arg Met Thr Asn Lys Cys Thr Tyr Leu Pro Thr Glu Asp Val 
        515                 520                 525             


Leu Pro Lys Asp Ser Leu Cys Tyr Gln Lys Phe Met Val Leu Asn Glu 
    530                 535                 540                 


Ile Asn Asn Leu Gln Ile Asp Gly Leu Lys Ile Pro Val Lys Ala Lys 
545                 550                 555                 560 


Gln Gly Ile Tyr His Glu Leu Phe Glu Lys Asn Lys Lys Val Lys Arg 
                565                 570                 575     


Lys Asp Ile Glu Glu Tyr Leu Ile Arg Asn Asn Tyr Leu Asp Lys Asp 
            580                 585                 590         


Ser Thr Glu Leu Ile Ser Gly Ile Asp Glu Gln Ile His Ser Gly Leu 
        595                 600                 605             


Ser Ser Tyr Cys Ala Phe Phe Asn Leu Leu Asn Lys Lys Ile Leu Ser 
    610                 615                 620                 


Glu Asp Asp Val Glu Arg Ile Ile Glu Arg Ala Ser Tyr Ala Glu Asp 
625                 630                 635                 640 


Lys Ser Arg Val Ile Lys Trp Leu Arg Lys Glu Tyr Pro Gly Leu Ser 
                645                 650                 655     


Glu Asp Asp Ile Arg Tyr Ile Ala Arg Ile Lys Ile Arg Asp Phe Gly 
            660                 665                 670         


Arg Leu Ser Lys Thr Phe Leu Thr Gly Leu Lys Gly Cys Asp Val Ser 
        675                 680                 685             


Lys Gly Phe Asp Gly Gln Asp Val Glu Met Ile Ser Ile Leu Gln Thr 
    690                 695                 700                 


Met Trp Glu Thr Asn Asp Asn Leu Met Glu Ile Leu Ser Asp Lys Tyr 
705                 710                 715                 720 


Thr Phe Leu Glu Ala Leu Glu Ala Phe Thr Gln Glu Tyr Tyr Thr Gly 
                725                 730                 735     


Lys Lys Lys Ser Leu Lys Glu Arg Leu Asp Glu Met Arg Val Ser Asn 
            740                 745                 750         


Ser Val Arg Arg Pro Val Tyr Arg Thr Leu Ala Val Val Lys Asp Ile 
        755                 760                 765             


Glu Lys Ala Phe Gly Lys Pro Asn Lys Ile Phe Val Glu Met Thr Arg 
    770                 775                 780                 


Gly Gly Gln Ala Asp Gln Lys Gly Lys Arg Ile Lys Ser Arg Lys Gln 
785                 790                 795                 800 


Gln Ile Leu Asp Leu Tyr Asp Lys Cys Lys Asp Asp Val His Asp Leu 
                805                 810                 815     


Met Trp Gln Leu Glu Ser Leu Gly Glu Tyr Ala Asp Ser Arg Leu Gln 
            820                 825                 830         


Ser Asp Arg Leu Tyr Leu Tyr Phe Met Gln Phe Gly Lys Cys Ala Tyr 
        835                 840                 845             


Ser Gly Ala His Ile Gly Phe Glu Gln Leu Met Ala Gly Ser Val Glu 
    850                 855                 860                 


Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Tyr Val Lys Asp Asp Ser 
865                 870                 875                 880 


Leu Asn Asn Lys Val Leu Val Leu Ser Asp Leu Asn Gly Thr Lys Asp 
                885                 890                 895     


Asn Ile Tyr Pro Val Ser Pro Glu Ile Arg Gly Lys Met Gln Gly Leu 
            900                 905                 910         


Trp Ser Trp Trp Asn His Val Gly Ala Ile Ser Asp Glu Lys Tyr Lys 
        915                 920                 925             


Arg Leu Ile Arg Ser Ala Pro Phe Thr Asp Glu Glu Lys Met Gly Phe 
    930                 935                 940                 


Ile Asn Arg Gln Leu Thr Glu Thr Ser Gln Ser Thr Lys Phe Ile Ala 
945                 950                 955                 960 


Asp Leu Leu Lys Glu Arg Phe Pro Asp Ala Glu Val Val Tyr Val Lys 
                965                 970                 975     


Ala Gly Leu Val Ser Asp Phe Arg His Glu Phe Asp Leu Pro Lys Ser 
            980                 985                 990         


Arg Ser Tyr Asn Asp Leu His Asn  Ala Ala Asp Ala Phe  Leu Ser Val 
        995                 1000                 1005             


Val Ala  Gly Asn Val Tyr Asp  Met Arg Phe Ser Lys  Lys Trp Phe 
    1010                 1015                 1020             


Arg Ile  Asp Glu Lys Tyr Ser  Val Lys Val Lys Thr  Ile Phe Thr 
    1025                 1030                 1035             


His Glu  Val Lys Cys Arg Gly  Asp Ile Ile Trp Asp  Gly Met Pro 
    1040                 1045                 1050             


Met Leu  Glu Lys Val Glu Asp  Thr Ala Arg Lys Asn  Thr Ala His 
    1055                 1060                 1065             


Phe Val  Lys Tyr Ala Ala Phe  Lys Thr Gly Gly Leu  Phe Asp Gln 
    1070                 1075                 1080             


Asn Pro  Val Lys Lys Gly Thr  Gly Leu Ile Pro Leu  Lys Ala Gly 
    1085                 1090                 1095             


Leu Pro  Thr Glu Lys Tyr Gly  Gly Tyr Asn Lys Ala  Gly Ile Met 
    1100                 1105                 1110             


Phe Leu  Ile Pro Val Arg Tyr  Asn Ile Gly Lys Lys  Ser Glu Ile 
    1115                 1120                 1125             


Leu Ile  Leu Pro Val Glu Leu  Met His Gly Lys His  Phe Leu Glu 
    1130                 1135                 1140             


Asp Lys  Val Phe Ala Arg Asn  Tyr Thr Phe Thr Arg  Leu Glu Asn 
    1145                 1150                 1155             


Ile Leu  Gly Lys Lys Val Gly  Glu Val Ser Phe Pro  Met Gly Met 
    1160                 1165                 1170             


Arg Pro  Trp Lys Ile Asn Thr  Met Leu Ser Phe Asp  Gly Phe Arg 
    1175                 1180                 1185             


Val Cys  Leu Thr Gly Thr Ser  Gly Arg Gly Lys Cys  Leu Val Ala 
    1190                 1195                 1200             


Gln Pro  Val Met Gln Phe Ser  Ala Asp Glu His Trp  Arg Phe Tyr 
    1205                 1210                 1215             


Val Lys  Arg Leu Glu Arg Phe  Val Glu Lys Ile Gly  Ile Asn Pro 
    1220                 1225                 1230             


Asp Leu  Leu Tyr Asp Lys Asp  His Asp Lys Val Ser  Lys Asp Glu 
    1235                 1240                 1245             


Asn Leu  Lys Leu Tyr Glu Leu  Tyr Val Asp Lys Leu  Gln Asn Thr 
    1250                 1255                 1260             


Ile Tyr  Lys Lys Arg Val Asn  Ser Pro Val Gln Thr  Leu Leu Lys 
    1265                 1270                 1275             


Gly Lys  Glu Glu Phe Lys Lys  Leu Ser Ile Glu Glu  Gln Cys Gln 
    1280                 1285                 1290             


Val Leu  Leu Asn Ile Gln Ser  Val Phe Gly Arg Met  Thr Gly Gly 
    1295                 1300                 1305             


Cys Asp  Leu Thr Leu Ile Gly  Gly Ser Ala His Ser  Ala Ala Thr 
    1310                 1315                 1320             


Gly Ser  Leu Ser Thr Thr Ile  Ser Asn Trp Lys Lys  Lys Tyr Ser 
    1325                 1330                 1335             


Thr Val  Arg Ile Ile Asp Gln  Ser Pro Ser Gly Met  Trp Glu Lys 
    1340                 1345                 1350             


Ser Ser  Glu Asn Leu Leu Asp  Phe Leu 
    1355                 1360         


<210>  963
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  963

Met Thr Lys Leu Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg 
            20                  25                  30          


Leu Lys Gly Lys Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys 
        35                  40                  45              


Ala Ala Ala Asp Arg Arg Ala Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Asn Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His 
65                  70                  75                  80  


Ile Thr Pro Thr Asp Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Ala Arg Tyr Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Gln Gln Tyr Pro Thr Met Tyr 
        115                 120                 125             


His Leu Arg Asn Arg Leu Leu Thr Asp Pro Ser Lys Ala Asp Val Arg 
    130                 135                 140                 


Glu Ile Tyr Phe Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Ser Gly Tyr Ala Lys Asp Phe Asn Thr Asn Lys Val Ala Leu 
                165                 170                 175     


Asn Glu Ile Phe Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Asp Ile Thr Phe Asp Glu Asn Lys Val Asn Glu Phe Lys Thr 
        195                 200                 205             


Val Leu Leu Asp Glu Lys Ala Thr Pro Ser Asp Thr Gln Arg Ala Leu 
    210                 215                 220                 


Val Asn Leu Leu Leu Ala Glu Asp Gly Asp Lys Asp Ile Leu Lys Gln 
225                 230                 235                 240 


Gln Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Val Val Gly Leu Lys 
                245                 250                 255     


Met Lys Leu Asn Val Ala Leu Gly Thr Glu Val Asp Ser Ser Glu Ala 
            260                 265                 270         


Thr Ala Trp Asn Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp Ala Gly 
        275                 280                 285             


Ile Glu Ser Ala Met Thr Asp Glu Gly Thr Glu Ile Leu Asp Gln Ile 
    290                 295                 300                 


Arg Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Glu Gln His His Glu 
                325                 330                 335     


Asp Leu Glu Leu Phe Lys Asp Tyr Leu Lys Gln Leu Glu Asp Asp Gly 
            340                 345                 350         


Met Ala Lys Val Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Val Arg Glu Asp Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Asn Ala Lys Ser Pro Glu Leu Leu Glu Arg 
385                 390                 395                 400 


Leu Val Gln Pro Asp Phe Met Leu Lys Gln Arg Asn Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Gln Ile Ile Lys 
            420                 425                 430         


Asn Gln Ser Val Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Gln Ser Met Pro Tyr Gln Leu Asp Glu Leu Leu Asn Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Ala Lys Glu Gln Lys 
465                 470                 475                 480 


Ala Ala Gln Gly Gly Val Phe Ala Trp Met Val Arg Lys Asp Pro Asp 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Ser Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asp Asn Lys Leu 
545                 550                 555                 560 


Ser Phe Asp Gln Lys Gln Gln Val Leu Gly Glu Leu Phe Glu Arg His 
                565                 570                 575     


Asn Thr Val Thr Ile Lys Gln Phe Val Asp Asn Leu Leu Ala His Gly 
            580                 585                 590         


Asp Tyr Asp Arg Ile Pro Glu Val Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr Asn Gln Leu Lys Gly Ile Leu His 
    610                 615                 620                 


Glu Ala Ile Asp Asp Ser Asp Lys Gln Thr Asp Ile Glu Lys Ile Ile 
625                 630                 635                 640 


Ala Trp Ser Thr Ile Phe Glu Asp Lys Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Asn Glu Ile Ser Trp Leu Thr Ala Gly Met Ile Asn Ser Leu Ala Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser His Lys Leu Leu Asn Glu 
        675                 680                 685             


Leu Thr Leu Ser Thr Gly Arg Thr Val Phe Glu Glu Leu Leu Ile Ser 
    690                 695                 700                 


Ser His Asn Leu Met Gln Ile Leu Thr Asp Glu Val Leu Lys Gln Lys 
705                 710                 715                 720 


Ile Ala Glu Leu Asn Ala His Glu Leu Lys Ala Ser Gly Ile Tyr Ala 
                725                 730                 735     


Ala Ile Asp Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Ser Val Ile Asp Asp Ile Thr Asn Ala Ala Gly Gln Ala 
        755                 760                 765             


Pro Asn Trp Leu Tyr Ile Glu Thr Ala Asp Gly Asp Gly Val Pro Gly 
    770                 775                 780                 


Lys Arg Thr Asn Ser Arg Gln Arg Lys Ile Gln Glu Ile Tyr Ala Asn 
785                 790                 795                 800 


Ala Ala Gln Glu Leu Ile Thr Gly Ser Val Arg Gly Glu Leu Glu Asp 
                805                 810                 815     


Lys Ile Ala Ser Arg Ala Asp Phe Thr Asp Arg Leu Val Leu Tyr Phe 
            820                 825                 830         


Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Glu Pro Leu Asn Ile Asp 
        835                 840                 845             


Asn Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu Ile 
    850                 855                 860                 


Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val His Pro Leu Val Asn 
865                 870                 875                 880 


Arg Glu Lys Asp Thr Ile Phe Ala Ser Thr Arg Phe Ala Lys Gln Met 
                885                 890                 895     


Asn Gly Met Trp Arg Gln Trp His Ala Ala Gly Leu Ile Ser Ser Arg 
            900                 905                 910         


Lys Leu Arg Asn Leu Gln Met Arg Pro Asp Glu Ile Asp Lys Tyr Ala 
        915                 920                 925             


Ser Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys 
    930                 935                 940                 


Leu Thr Glu Gln Ile Val Ala Asp Gln Tyr Pro Asp Ala Lys Ile Ile 
945                 950                 955                 960 


Ala Val Lys Ala Gly Leu Ser Ser Gln Leu Arg Lys Glu Leu Asp Phe 
                965                 970                 975     


Pro Lys Asn Arg Glu Val Asn His Tyr His His Ala Phe Asp Ala Phe 
            980                 985                 990         


Leu Ala Ala Arg Ile Gly Thr Tyr  Leu Leu Lys Arg Tyr  Pro Asn Leu 
        995                 1000                 1005             


Glu Pro  Phe Phe Thr Tyr Gly  Lys Phe Lys Lys Thr  Glu Val Lys 
    1010                 1015                 1020             


Lys Leu  Lys Ser Leu Lys Ser  Phe Asn Phe Ile Arg  Asp Met Thr 
    1025                 1030                 1035             


His Ala  Lys Asp Lys Ile Val  Ala Lys Glu Thr Gly  Glu Ile Val 
    1040                 1045                 1050             


Trp Asp  Asn Ala Ser Asp Ile  Asn Glu Leu Asp Arg  Ile Tyr Asn 
    1055                 1060                 1065             


Phe Lys  Arg Met Leu Ile Thr  His Glu Val Arg Phe  Glu Thr Ala 
    1070                 1075                 1080             


Ser Leu  Phe Lys Gln Thr Leu  Tyr Ala Ala Lys Asn  Ser Lys Asn 
    1085                 1090                 1095             


Arg Gly  Gly Ser Arg Gln Leu  Ile Pro Lys Lys Lys  Gly Tyr Leu 
    1100                 1105                 1110             


Val Asp  Ile Tyr Gly Gly Tyr  Thr Gln Glu Thr Gly  Ser Tyr Leu 
    1115                 1120                 1125             


Ser Val  Val Arg Leu Thr Lys  Lys Ala Met Tyr Ala  Val Val Lys 
    1130                 1135                 1140             


Val Ser  Thr Arg Asp Ala Ala  Lys Leu Ala Val Ala  Lys Ser Ile 
    1145                 1150                 1155             


Ser Glu  Gln Lys Glu Asn Glu  Thr Leu Lys Lys Ile  Ile Asp Gly 
    1160                 1165                 1170             


Lys Leu  Ser Lys Thr Ser Lys  Lys Gly Lys Thr Thr  His Gln Leu 
    1175                 1180                 1185             


Phe Glu  Ile Val Leu Pro Arg  Val Gly Gln Lys Thr  Leu Phe Lys 
    1190                 1195                 1200             


Asn Ser  Lys Tyr Asn Gln Phe  Leu Val Asn Ser Asp  Thr Tyr Met 
    1205                 1210                 1215             


His Asn  Tyr Gln Glu Leu Trp  Met Pro Arg Glu Tyr  Gln Arg Met 
    1220                 1225                 1230             


Trp Lys  Asp Ile Leu Leu Ser  Asn His Gly Asp Ala  Gln Leu Glu 
    1235                 1240                 1245             


Gly Gln  Leu Asp Gln Ile Phe  Lys Phe Ile Val Ser  Gln Val Asn 
    1250                 1255                 1260             


Ser Tyr  Phe Asn Leu Tyr Asp  Ile Asn Gln Phe Arg  Lys Lys Ile 
    1265                 1270                 1275             


Ala Asp  Ala Thr Asp Lys Phe  Ala Lys Leu Pro Thr  His Asp Thr 
    1280                 1285                 1290             


Asp Asn  Ala Arg Gly Lys Ile  Ala Thr Ile Gly Gln  Leu Leu Ile 
    1295                 1300                 1305             


Gly Leu  Gln Ala Asn Ala Met  Arg Ser Asp Leu Arg  Asn Leu Asp 
    1310                 1315                 1320             


Ile Lys  Thr Pro Phe Gly Leu  Leu Gln Ile Gly Thr  Gly Ile Thr 
    1325                 1330                 1335             


Leu Asp  Leu Asp Thr Ser Ile  Ile Tyr Gln Ser Pro  Thr Gly Leu 
    1340                 1345                 1350             


Phe Glu  Arg Glu Val Pro Leu  Thr Asp Leu 
    1355                 1360             


<210>  964
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  964

Met Arg Gly Asn Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Trp Ala Val Ile Asp Asp Asn Ala Lys Val Met Arg Ala Lys Gly 
            20                  25                  30          


Lys Thr Ala Ile Gly Ala Arg Leu Tyr Asn Glu Gly Gln Thr Ala Ala 
        35                  40                  45              


Asp Arg Arg Leu Phe Arg Thr Thr Arg Arg Arg Leu Asn Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Lys Leu Leu Asp Glu Ile Phe Asp Pro Tyr Ile Thr Pro 
65                  70                  75                  80  


Val Asp Ala Ala Phe Phe Ala Arg Leu Lys Gln Ser Asn Leu Ser Pro 
                85                  90                  95      


Lys Asp Gln Asn Arg Asp Trp Thr Gly Ser Met Leu Phe Pro Asp Arg 
            100                 105                 110         


Thr Asp Arg Lys Phe His Lys Asp Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


His Lys Leu Met Thr Glu Asp His Gln Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn Thr 
145                 150                 155                 160 


Thr Pro Val Ser Ser Phe Ser Val Ser Asp Val Lys Phe Asp Glu Gln 
                165                 170                 175     


Phe Asp Arg Leu Asn Glu Leu Tyr Ala Ala Val Ser Pro Asp Glu Pro 
            180                 185                 190         


Phe Ala Ile Asn Gly Asp Asn Ser Lys Ala Ile Glu Thr Leu Leu Leu 
        195                 200                 205             


Ser Asp Glu Thr Lys Lys Phe Asp Lys Gln Lys Gln Ser Ala Lys Leu 
    210                 215                 220                 


Ile Leu Ser Glu Thr Gly Asp Lys Ala Leu Asp Lys Glu Asn Thr Lys 
225                 230                 235                 240 


Val Ala Thr Gln Ile Ser Lys Ala Ile Leu Gly Tyr Lys Thr Gln Leu 
                245                 250                 255     


Asp Val Leu Leu Lys Tyr Asp Ala Ile Asn Ser Ser Ala Trp Lys Ile 
            260                 265                 270         


Glu Leu Asp Asp Glu Asn Ile Asp Asp Thr Leu Pro Gly Leu Leu Ser 
        275                 280                 285             


Gln Leu Asp Glu Asn Arg Gln Glu Met Val Ser Ile Ile Gln Asn Leu 
    290                 295                 300                 


Tyr Ala Gln Val Thr Leu Asn Gln Ile Val Pro Asn Gly Met Ser Leu 
305                 310                 315                 320 


Ser Glu Ser Met Ile Glu Lys Tyr Asn Ala His Arg Asp His Leu Lys 
                325                 330                 335     


Leu Leu Lys Gln Leu Glu Asp Lys Ile Asp Asp Pro Lys Lys Lys Lys 
            340                 345                 350         


Gly Leu Glu Ala Ala Tyr Asp Ala Tyr Val Gly Lys Asn Met Lys Val 
        355                 360                 365             


Ala Asn Gln Asp Val Phe Tyr Ala Ser Val Lys Lys Asn Leu Asp Asp 
    370                 375                 380                 


Ser Asp Leu Ala Lys Gln Ile Ala Asp Leu Ile Glu Ala Gln Lys Phe 
385                 390                 395                 400 


Met Pro Lys Gln Arg Thr Ser Gln Asn Gly Val Ile Pro His Gln Leu 
                405                 410                 415     


His Gln Leu Glu Leu Asp Gln Ile Ile Glu Asn Gln Ser Lys Tyr Tyr 
            420                 425                 430         


Pro Trp Leu Thr Ala Ala Asn Pro Asn Pro Lys Arg Ile His Gln Ala 
        435                 440                 445             


Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Ile Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Leu Val Thr Pro Glu Glu Gln Glu Lys Ser Ala Asn Gln Val 
465                 470                 475                 480 


Phe Ala Trp Met Lys Arg Lys Gln Pro Gly Thr Ile Thr Pro Trp Asn 
                485                 490                 495     


Phe Asp Gln Lys Val Asp Arg Met Ala Ser Ala Asn Arg Phe Ile Arg 
            500                 505                 510         


Arg Met Thr Thr Lys Asp Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro 
        515                 520                 525             


Asp Ala Ser Leu Ile Tyr Glu Arg Phe Lys Val Leu Asn Glu Leu Asn 
    530                 535                 540                 


Leu Val Lys Ala Asn Tyr His Arg Leu Ala Pro Ala Gln Lys Gln Ala 
545                 550                 555                 560 


Ala Tyr Arg Asp Leu Phe Glu Asn Gln Lys Ser Ile Ser Thr Lys Lys 
                565                 570                 575     


Leu Gln Asn Tyr Leu Val Asp Gln Gly Tyr Phe Thr Ala Lys Pro Lys 
            580                 585                 590         


Ile Thr Gly Leu Ser Asp Pro Glu Arg Phe Asn Ser Ser Leu Gly Thr 
        595                 600                 605             


Tyr Ile Asp Phe Lys Glu Ile Phe Gly Asp Gln Val Asp Asn Pro Asp 
    610                 615                 620                 


Arg Gln Asp Asp Tyr Glu Lys Ile Ile Glu Trp Ser Thr Val Phe Glu 
625                 630                 635                 640 


Asp Arg Gln Ile Tyr Glu Ala Lys Leu Arg Thr Leu Asp Trp Leu Thr 
                645                 650                 655     


Glu Asp Gln Ile Gln Ala Leu Lys Thr Lys Arg Tyr Asn Gly Trp Gly 
            660                 665                 670         


Arg Leu Ser Lys Lys Leu Leu Thr Gly Ile Val Asp Glu Asn Gly Glu 
        675                 680                 685             


Arg Ile Ile Asp Val Leu Trp Asn Thr Asn Gln Asn Phe Met Gln Val 
    690                 695                 700                 


Gln Ser Gln Glu Ser Phe Ala Lys Arg Ile His Glu Ala Asn Ala Lys 
705                 710                 715                 720 


Gln Met Lys Val Thr Gly Val Glu Asp Ile Leu Ala Asp Ala Tyr Thr 
                725                 730                 735     


Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Ile Arg Val Val Asp 
            740                 745                 750         


Asp Ile Gln Lys Ala Met Gly Gly Val Ala Pro Thr Ser Val Ser Ile 
        755                 760                 765             


Glu Phe Thr Arg Ser Glu Asp Gln Asn Pro Arg Arg Thr Gln Ser Arg 
    770                 775                 780                 


Glu Ser Gln Leu Gln Asn Thr Phe Lys Val Asp Ala Lys Glu Ile Val 
785                 790                 795                 800 


Asp Ser Asp Leu Val Thr Glu Leu Glu Thr Val Ser Lys Ser Lys Lys 
                805                 810                 815     


Gly Leu Thr Asp Arg Leu Tyr Leu Tyr Phe Thr Gln Leu Gly Lys Asp 
            820                 825                 830         


Met Tyr Thr Gly Asp Pro Leu Asn Ile Asp Glu Leu Ser Thr Tyr Asp 
        835                 840                 845             


Ile Asp His Ile Leu Pro Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp 
    850                 855                 860                 


Asn Arg Val Leu Val Ser Lys Ala Val Asn Asn Gly Lys Ser Asn Asn 
865                 870                 875                 880 


Val Pro Val Lys Leu Phe Gly Gly Lys Met Gly Gly Phe Trp Lys Glu 
                885                 890                 895     


Leu Ala Asp His Arg Leu Ile Ser Lys Arg Lys Leu Lys His Leu Leu 
            900                 905                 910         


Thr Asp Pro Asp Thr Ile Asp Lys Tyr Thr Met Arg Gly Phe Ile Arg 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Val Ala Gln Ile 
    930                 935                 940                 


Leu Gly Asp Lys Tyr Gln Asn Asp Asp Thr Lys Ile Ile Glu Val Thr 
945                 950                 955                 960 


Ala Lys Met Asn His Gln Met Arg Glu Asn Phe Lys Leu Ile Lys Asn 
                965                 970                 975     


Arg Glu Val Asn Asp Tyr His His Ala Val Asp Ala Tyr Leu Thr Ala 
            980                 985                 990         


Phe Val Gly Gln Tyr Leu Tyr Lys  Arg Tyr Pro Lys Leu  Arg Pro Tyr 
        995                 1000                 1005             


Phe Val  Tyr Gly Asn Phe Lys  Lys Phe Asn Glu Asp  His Val Ser 
    1010                 1015                 1020             


Phe Arg  Tyr Phe Asn Phe Leu  His Glu Leu Met Asp  Glu Asn Asn 
    1025                 1030                 1035             


Lys Arg  Ile Val Glu Pro Glu  Thr Gly Glu Val Val  Leu Glu Arg 
    1040                 1045                 1050             


Glu Pro  Ala Ile Ala Tyr Met  Lys Arg Val Tyr His  Phe Lys Phe 
    1055                 1060                 1065             


Met Leu  Ile Ser Gln Glu Val  Tyr Thr Arg Arg Asp  Ala Met Phe 
    1070                 1075                 1080             


Asn Gln  Thr Ile Tyr Pro Ala  Lys Glu Ala Asn Arg  Arg Lys Leu 
    1085                 1090                 1095             


Ile Ala  Ile Lys His Gly Lys  Pro Val Asp Val Tyr  Gly Gly Tyr 
    1100                 1105                 1110             


Ser Gly  Ser Val Asp Ala Tyr  Met Ala Ile Val Arg  Ile His Asp 
    1115                 1120                 1125             


Lys Lys  Gly Asp Lys Tyr Lys  Val Val Gly Val Pro  Met Arg Ala 
    1130                 1135                 1140             


Leu Glu  Ile Leu Asn Ala Thr  His Asp Lys Lys Asp  Tyr Asp Ala 
    1145                 1150                 1155             


Lys Leu  Lys Gln Ile Leu Val  Pro Lys Phe Thr Lys  Val Lys Lys 
    1160                 1165                 1170             


Ser Arg  Lys Thr Gly Glu Ile  Thr Lys Lys Val Glu  Asp Phe Glu 
    1175                 1180                 1185             


Ile Val  Leu Gly Lys Val Met  Tyr Arg Gln Leu Val  Ile Asp Gly 
    1190                 1195                 1200             


Asp Lys  Lys Phe Met Val Gly  Ser Ser Thr Tyr Leu  Tyr Asn Ala 
    1205                 1210                 1215             


Arg Gln  Leu Val Leu Ser Asp  Ser Ser Leu Asp Thr  Leu Ala Ser 
    1220                 1225                 1230             


Lys Gly  Thr Leu Asp Ser Asp  Ala Asp Ser Lys Arg  Leu Val Ala 
    1235                 1240                 1245             


Val Tyr  Asp Glu Ile Val Glu  Lys Val Asp Lys Tyr  Phe Pro Leu 
    1250                 1255                 1260             


Tyr Asp  Lys Asn Ala Phe Arg  Ser Gly Leu His Lys  Gly Arg Asp 
    1265                 1270                 1275             


Lys Tyr  Leu Leu Leu Pro Asn  Arg Ser Thr Phe Asn  Gly Ala Lys 
    1280                 1285                 1290             


Lys Val  His Ser Gly Lys Arg  Glu Ile Leu Asn Glu  Ile Leu Asn 
    1295                 1300                 1305             


Gly Leu  His Ala Asn Ala Thr  Leu Gly Gly Leu Lys  Asp Leu Lys 
    1310                 1315                 1320             


Ile Ser  Thr Pro Phe Gly Lys  Met Gln Gly Gln Ser  Gly Ile Pro 
    1325                 1330                 1335             


Leu Ser  Ser Asn Thr Ile Ile  Cys Tyr Gln Ser Pro  Thr Gly Leu 
    1340                 1345                 1350             


Phe Glu  His Arg Val Ser Leu  Lys Asp Leu 
    1355                 1360             


<210>  965
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  965

Met Glu Asn Asn Arg Tyr Asn Val Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Phe Ala Val Thr Asp Glu Gln Asp Glu Leu Val Gln Val Lys 
            20                  25                  30          


Gly Lys Asn Tyr Ile Gly Val Arg Leu Phe Lys Glu Gly Gln Thr Ala 
        35                  40                  45              


Ser Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Gly Leu Leu Glu Glu Ile Phe Ala Pro Tyr Leu Ala 
65                  70                  75                  80  


Gln Gly Asp Pro Tyr Phe Leu Ala Arg Met Lys Glu Ser Asn Ile Ser 
                85                  90                  95      


Pro Arg Asp Glu Arg Lys Lys Tyr Tyr Gly Ser Leu Leu Phe Ser Asp 
            100                 105                 110         


Asp Thr Val Phe Tyr Asp Lys Tyr Pro Thr Val Tyr His Leu Arg His 
        115                 120                 125             


Ala Leu Met Thr Glu Asp Arg Lys Phe Asp Ile Arg Glu Ile Tyr Leu 
    130                 135                 140                 


Ala Val His His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Asn Thr 
145                 150                 155                 160 


Leu Ile Asn Glu Phe Ser Ser Lys Ala Val Asp Phe Lys Lys Asp Ala 
                165                 170                 175     


Asp Ile Leu Asn Asp Leu Phe Glu Gln Phe Tyr Ile Ser Asp Ile Asn 
            180                 185                 190         


Asp Ser Phe Gln Leu Asn Thr Ala Asp Met Asn Glu Ile Ser Glu Leu 
        195                 200                 205             


Leu Leu Asp Asn Ser Lys Thr Arg Leu Asp Arg Gln Arg Glu Ser Leu 
    210                 215                 220                 


Lys Lys Leu Ala Lys Lys Val Asp Asp Ile Ser Val Ala Lys Lys His 
225                 230                 235                 240 


Gln Lys Ile Ala Thr Glu Phe Ser Lys Ala Ile Leu Gly Asn Lys Ala 
                245                 250                 255     


Lys Phe Asp Val Ile Leu Asn Val Thr Thr Asp Ser Ser Asn Asp Trp 
            260                 265                 270         


Ser Phe Lys Leu Glu Asp Glu Asp Ala Asp Glu Lys Ile Val Val Leu 
        275                 280                 285             


Leu Glu Glu Leu Asn Thr Pro Gln Gln Glu Ala Leu Asn Ile Ile His 
    290                 295                 300                 


Gln Trp Tyr Ser Gln Ile Ser Leu Asn Gly Ile Val Pro Asp Gly Lys 
305                 310                 315                 320 


Ser Ile Ser Glu Ala Met Ile Ser Ser Tyr Glu Leu His Lys Glu His 
                325                 330                 335     


Leu Ala Leu Leu Lys Gln Gln Ile Asn Asn Thr Lys Asp Lys Lys Lys 
            340                 345                 350         


Ala His Gly Leu His Glu Ala Tyr Asn Glu Tyr Val His Gly Asn Asn 
        355                 360                 365             


Asn Lys Lys Leu Asn Gln Ala Asp Phe Tyr Ser Lys Val Lys Gln Asn 
    370                 375                 380                 


Leu Glu Ser Ser Leu Ile Thr Asp Glu Ile Leu Glu Leu Ile Asn Ala 
385                 390                 395                 400 


Glu Asn Phe Met Pro Lys Gln Arg Thr Ser Ala Asn Gly Val Ile Pro 
                405                 410                 415     


His Gln Leu His Gln Gln Glu Leu Asp Arg Ile Ile Gln Asn Gln Ala 
            420                 425                 430         


Lys Tyr Tyr Pro Phe Leu Ala Glu Thr Asn Pro Asn Glu Lys Arg Ser 
        435                 440                 445             


Lys Val Ala Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Val Pro 
    450                 455                 460                 


Tyr Tyr Val Gly Pro Leu Ile Thr Ala Thr Asp Gln Lys Lys Thr Ser 
465                 470                 475                 480 


Gly Lys Glu Phe Ala Trp Met Val Arg Lys Gln Pro Gly Arg Ile Thr 
                485                 490                 495     


Pro Trp Asn Phe Asp Glu Lys Val Asn Lys Met Glu Ser Ala Asn Thr 
            500                 505                 510         


Phe Ile Arg Arg Met Thr Thr Lys Asp Ser Tyr Leu Ile Ala Glu Asp 
        515                 520                 525             


Val Leu Pro Asp Glu Ser Leu Leu Tyr Gln Lys Phe Lys Val Leu Asn 
    530                 535                 540                 


Glu Leu Asn Asn Leu Arg Ala Asp Gly Arg Lys Leu Lys Val Ser Val 
545                 550                 555                 560 


Lys Gln Lys Val Phe Lys Glu Leu Phe Gln Gln Gln Lys Thr Ile Thr 
                565                 570                 575     


Ile Val Lys Leu Gln Asn Tyr Leu Lys Ala Glu Cys Ser Phe Leu Thr 
            580                 585                 590         


Thr Pro Glu Ile Ser Gly Leu Ser Lys Pro Glu His Phe Val Ser Ser 
        595                 600                 605             


Leu Ala Thr Tyr Ile Asp Leu Lys Lys Ile Phe Gly Glu Asp Ile Asp 
    610                 615                 620                 


Asn Pro Ala Arg Gln Asn Asp Phe Glu Arg Met Ile Glu Trp Ala Thr 
625                 630                 635                 640 


Ile Phe Glu Asp Lys Asn Ile Tyr Ser Glu Lys Leu Asn Glu Ile Lys 
                645                 650                 655     


Trp Leu Asn Asp Arg Gln Lys Lys Glu Leu Lys Asn Lys Asn Tyr His 
            660                 665                 670         


Gly Trp Gly Lys Leu Ser Ala Lys Leu Leu Lys Gly Leu Arg Asp Ser 
        675                 680                 685             


Asp Glu Cys Ser Ile Ile Glu His Met Trp Asn Thr Gln Gln Asn Phe 
    690                 695                 700                 


Met Gln Val Gln Ala Glu Glu Asp Phe Ala Gln Arg Ile Gln Glu Glu 
705                 710                 715                 720 


Asn Asn Gln His Leu Lys Gly Asn Thr Leu Glu Glu Val Leu Ala Asp 
                725                 730                 735     


Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Val Lys 
            740                 745                 750         


Val Ile Glu Asp Ile Gln Lys Ala Met Gly Gly Ile Ala Pro Gln Lys 
        755                 760                 765             


Ile Ser Ile Glu Phe Thr Arg Ala Pro Glu Glu Lys Pro Arg Arg Ser 
    770                 775                 780                 


Val Glu Arg Gln Gln Gln Ile Glu Lys Ile Tyr Lys Ser Val Ala Asn 
785                 790                 795                 800 


Glu Val Glu Asn Ala Gly Val Lys Asp Glu Leu Gln Arg Phe Ser Asp 
                805                 810                 815     


Arg Lys Gln Thr Leu Thr Asp Lys Tyr Phe Leu Tyr Phe Thr Gln Leu 
            820                 825                 830         


Gly Lys Asp Met Tyr Thr Gly Lys Ser Ile Asn Ile Asp Gln Ile Gly 
        835                 840                 845             


Asn Tyr Gln Ile Asp His Ile Leu Pro Gln Ala Phe Ile Lys Asp Asp 
    850                 855                 860                 


Ser Leu Asp Asn Arg Val Leu Val Ser Ala Ala Val Asn Asn Gly Lys 
865                 870                 875                 880 


Ser Asp Asn Val Pro Thr Lys Asn Asp Arg Tyr Lys Val Arg Glu His 
                885                 890                 895     


Gly Gln Arg Leu Tyr Asp Gln Gly Leu Ile Ser Lys Arg Lys Phe Asn 
            900                 905                 910         


Asn Leu Ile Thr Asp Pro Asp Ser Ile Asn Lys Phe Thr Ala Arg Gly 
        915                 920                 925             


Phe Val Lys Arg Gln Leu Val Glu Thr Ser Gln Val Ile Arg Leu Val 
    930                 935                 940                 


Ala Glu Phe Leu Tyr Asn Lys Tyr Gln Ser Lys Ser Glu Ile Ile Glu 
945                 950                 955                 960 


Val Arg Ala Lys Met Asn Ser Gln Leu Arg Lys Asp Phe Gly Leu Ile 
                965                 970                 975     


Lys Asn Arg Asn Val Asn Asp Tyr His His Ala Leu Asp Ala Tyr Leu 
            980                 985                 990         


Thr Thr Phe Ile Gly Asn Tyr Leu  Tyr Arg Arg Tyr Pro  Lys Leu Arg 
        995                 1000                 1005             


Ala Tyr  Phe Thr Tyr Gly Cys  Phe Lys Lys Val Asp  Asp Ser Gln 
    1010                 1015                 1020             


Leu Lys  Tyr Phe Asn Phe Leu  His Asp Ile Glu Asp  Asp Lys Lys 
    1025                 1030                 1035             


Lys Ile  Ile Lys Asp Asn Asp  Thr Gly Glu Ile Leu  Trp His Arg 
    1040                 1045                 1050             


Asp Glu  Gly Ile Ala Arg Phe  Lys Arg Ile Tyr Asn  Phe Lys Tyr 
    1055                 1060                 1065             


Met Leu  Val Thr His Glu Val  Leu Thr Arg Gln Gly  Ala Met Phe 
    1070                 1075                 1080             


Lys Gln  Thr Val Tyr Ser Ala  Ala Val Ala Gly Ser  Lys Lys Leu 
    1085                 1090                 1095             


Ile Pro  Val Lys Glu Asp Arg  Pro Ile Asp Ile Tyr  Gly Gly Tyr 
    1100                 1105                 1110             


Thr Asn  Asn Asn Asp Ala Phe  Leu Ala Ile Val Lys  Val Thr Lys 
    1115                 1120                 1125             


Lys Lys  Gln Tyr Met Val Val  Gly Ile Pro Gln Arg  Ala Val Val 
    1130                 1135                 1140             


Lys Leu  Asn Asp Ala Ala Lys  Gln Gly Ser Lys Ala  Tyr Gln Lys 
    1145                 1150                 1155             


Val Leu  Tyr Asn Val Ile Lys  Pro Gln Phe Ile Lys  Lys Lys Lys 
    1160                 1165                 1170             


Asp Arg  Lys Thr Gly Lys Ile  Thr Gln Lys Leu Glu  Asn Phe Glu 
    1175                 1180                 1185             


Ile Val  Val Ser Lys Thr Leu  Tyr Arg Gln Leu Ile  Ile Asp Gly 
    1190                 1195                 1200             


Asp Gln  Lys Tyr Met Leu Gly  Ser Ser Ala Tyr Lys  Tyr Asn Ala 
    1205                 1210                 1215             


Lys Gln  Leu Val Leu Ser Glu  Tyr Ala Leu Glu Val  Leu Asp Arg 
    1220                 1225                 1230             


Ser Ile  Phe Glu Lys Lys Glu  Tyr Thr Thr Val Asp  Leu Asp Asn 
    1235                 1240                 1245             


Val Tyr  Asp Glu Ile Leu Ala  Gln Val Asp Lys Tyr  Phe Ser Leu 
    1250                 1255                 1260             


Tyr Asp  Lys Asn Lys Phe Arg  Gln Lys Leu His Glu  Ser Arg Lys 
    1265                 1270                 1275             


Ile Phe  Thr Glu Leu Pro Ile  Asn Asn Leu Phe Ser  Gly Thr Lys 
    1280                 1285                 1290             


Leu Leu  Lys Ser Gly Lys Arg  Glu Thr Leu Asn Glu  Ile Leu Glu 
    1295                 1300                 1305             


Gly Leu  His Ala Asn Ala Thr  Lys Lys Asp Leu Lys  Ser Leu Lys 
    1310                 1315                 1320             


Phe Ser  Thr Pro Leu Gly Met  Ile Gln Tyr Pro Ser  Gly Ile Ile 
    1325                 1330                 1335             


Leu Ser  Glu Asn Ala Ile Leu  Cys Tyr Gln Ser Pro  Thr Gly Ile 
    1340                 1345                 1350             


Phe Glu  Arg Arg Val Thr Leu  Lys Asp Leu 
    1355                 1360             


<210>  966
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  966

Met Thr Lys Leu Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg 
            20                  25                  30          


Leu Lys Gly Lys Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys 
        35                  40                  45              


Ala Ala Ala Asp Arg Arg Ala Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Asn Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His 
65                  70                  75                  80  


Ile Thr Pro Thr Asp Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Ser Arg Tyr Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Lys Tyr Glu Asn Cys Pro Thr 
        115                 120                 125             


Met Tyr His Leu Arg Asn Arg Leu Met Thr Asp Pro Ser Lys Ala Asp 
    130                 135                 140                 


Val Arg Glu Ile Tyr Phe Ala Ile His His Ile Leu Lys Ser Arg Gly 
145                 150                 155                 160 


His Phe Leu Thr Pro Gly Asp Ala Lys Asp Phe Asn Thr Asn Lys Val 
                165                 170                 175     


Ala Val Asn Lys Ile Phe Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val 
            180                 185                 190         


Tyr Pro Asp Leu Asp Ile Thr Phe Asp Glu Asn Lys Val Asn Glu Phe 
        195                 200                 205             


Lys Thr Val Leu Leu Asn Glu Lys Ala Thr Pro Ser Asp Thr Gln Arg 
    210                 215                 220                 


Ala Leu Val Asn Leu Leu Leu Ala Lys Glu Gly Asp Arg Asp Ile Leu 
225                 230                 235                 240 


Lys Gln Gln Lys Gln Ala Leu Thr Glu Phe Ala Lys Ala Val Val Gly 
                245                 250                 255     


Leu Lys Thr Lys Leu Asn Val Ala Leu Gly Thr Glu Val Asp Ser Ser 
            260                 265                 270         


Glu Ala Thr Ala Trp Asn Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp 
        275                 280                 285             


Thr Gly Ile Glu Ser Ala Met Thr Asp Glu Gly Thr Glu Ile Leu Asp 
    290                 295                 300                 


Gln Ile Arg Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro 
305                 310                 315                 320 


Ala Gly Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Ala Gln His 
                325                 330                 335     


His Glu Asp Leu Glu Leu Phe Lys Asp Tyr Leu Lys Gln Leu Glu Asp 
            340                 345                 350         


Asp Gly Thr Ala Lys Ala Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp 
        355                 360                 365             


Gly Asp Asp Ala Lys Pro Phe Val Arg Glu Asp Phe Val Lys Ala Leu 
    370                 375                 380                 


Thr Lys Glu Val Thr Ala His Pro Asn Ala Lys Ser Pro Glu Leu Leu 
385                 390                 395                 400 


Glu Arg Leu Ala Gln Pro Asp Phe Met Leu Lys Gln Arg Asn Lys Ala 
                405                 410                 415     


Asn Gly Ala Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Gln Ile 
            420                 425                 430         


Ile Lys Asn Gln Ser Val Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro 
        435                 440                 445             


Val Glu Lys His Arg Lys Ser Met Pro Tyr Gln Leu Asp Glu Leu Leu 
    450                 455                 460                 


Asn Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Ala Lys Glu 
465                 470                 475                 480 


Gln Lys Ala Ala Gln Gly Gly Val Phe Ala Trp Met Val Arg Lys Asp 
                485                 490                 495     


Pro Asp Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg 
            500                 505                 510         


Glu Ala Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr 
        515                 520                 525             


Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln 
    530                 535                 540                 


Arg Tyr Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asn Glu 
545                 550                 555                 560 


Lys Leu Ser Ile Glu Gln Lys Gln Gln Val Ile Arg Glu Leu Phe Glu 
                565                 570                 575     


Arg His Asn Ser Val Thr Ile Lys Gln Phe Ala Glu Asn Leu Arg Ala 
            580                 585                 590         


His Gly Asp Tyr Ala His Met Pro Glu Ile Arg Gly Leu Ala Asp Glu 
        595                 600                 605             


Lys Arg Phe Leu Ser Ser Leu Ser Thr Tyr Arg Gln Leu Lys Ser Leu 
    610                 615                 620                 


Leu Pro Glu Ala Ile Asp Asp Pro Ala Lys Gln Ala Asp Ile Glu Asn 
625                 630                 635                 640 


Ile Ile Ala Trp Ser Thr Val Phe Glu Asp Ala Ala Ile Phe Lys Thr 
                645                 650                 655     


Lys Leu Lys Glu Ile Asn Trp Leu Asp Ser Gln Thr Ile Thr Lys Leu 
            660                 665                 670         


Ser Asn Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser His Lys Phe Leu 
        675                 680                 685             


Asn Gly Leu Thr Leu Gly Asn Gly His Thr Ile Ile Gln Glu Leu Phe 
    690                 695                 700                 


Leu Ser Asn Asn Asn Leu Met Gln Ile Leu Thr Asp Glu Thr Leu Gln 
705                 710                 715                 720 


Lys Lys Met Thr Glu Leu Asn Ala Asp Lys Leu Lys Ala Ala Asn Ile 
                725                 730                 735     


Asn Asp Ala Ile Asp Asp Val Tyr Thr Ser Pro Ser Asn Lys Lys Ala 
            740                 745                 750         


Leu Arg Gln Val Leu Arg Val Ile Glu Asp Ile Lys Arg Ala Ala Asp 
        755                 760                 765             


Gly Gln Asp Pro Ser Trp Leu Tyr Val Glu Thr Ala Asp Gly Gly Gly 
    770                 775                 780                 


Thr Pro Gly Lys Arg Thr Arg Ala Arg Gln His Gln Leu Gln Glu Ile 
785                 790                 795                 800 


Tyr Ala Asn Ala Ala His Glu Leu Ile Asp Ser Ala Val Arg Gly Glu 
                805                 810                 815     


Leu Glu Asp Lys Ile Ala Asp Lys Ala Asp Phe Asn Asp Arg Leu Val 
            820                 825                 830         


Leu Tyr Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Thr Pro Leu 
        835                 840                 845             


Asn Ile Asp Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln 
    850                 855                 860                 


Ser Leu Ile Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val His Ala 
865                 870                 875                 880 


Ile Ile Asn Arg Glu Lys Asn Ala Thr Phe Ala Ser Thr Ile Tyr Ala 
                885                 890                 895     


Gln Lys Met Asn Ala Thr Trp Arg Gln Trp His Glu Ala Gly Leu Ile 
            900                 905                 910         


Ser Gly Arg Lys Leu Arg Asn Leu Gln Met Arg Pro Asp Gln Ile Asp 
        915                 920                 925             


Lys Tyr Ala Ser Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Ile Ile Lys Leu Thr Glu Gln Ile Val Ala Ala Gln Tyr Pro Glu Thr 
945                 950                 955                 960 


Lys Ile Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu 
                965                 970                 975     


Leu Glu Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe 
            980                 985                 990         


Asp Ala Phe Leu Ala Ala Arg Ile  Gly Thr Tyr Leu Leu  Lys Arg Phe 
        995                 1000                 1005             


Pro Asn  Leu Gln Ala Phe Phe  Thr Tyr Gly Lys Phe  Lys Lys Ala 
    1010                 1015                 1020             


Asp Val  Lys Lys Leu Arg Gly  Phe Asn Phe Ile Arg  Asp Ile Thr 
    1025                 1030                 1035             


His Ala  Glu Asp Lys Ile Val  Val Lys Asp Thr Gly  Glu Val Ile 
    1040                 1045                 1050             


Trp Asp  Lys Lys Arg Asp Val  Asp Glu Leu Asp Arg  Ile Tyr Asn 
    1055                 1060                 1065             


Phe Lys  Arg Met Leu Ile Thr  His Glu Val His Phe  Glu Lys Ala 
    1070                 1075                 1080             


Asp Leu  Phe Lys Gln Thr Val  Tyr Gly Ala Lys Asp  Ser Lys Glu 
    1085                 1090                 1095             


Ala Gly  Gly Lys Lys Gln Leu  Ile Ser Lys Lys Lys  Gly Tyr Pro 
    1100                 1105                 1110             


Val Asp  Ile Tyr Gly Gly Tyr  Thr Gln Glu Thr Gly  Ser Tyr Leu 
    1115                 1120                 1125             


Ser Val  Val Arg Leu Thr Lys  Lys Ala Met Tyr Ala  Val Val Lys 
    1130                 1135                 1140             


Val Ser  Thr Arg Asp Ala Ala  Lys Leu Ala Val Ala  Lys Ser Ile 
    1145                 1150                 1155             


Ser Glu  Gln Lys Glu Asn Glu  Thr Leu Asn Asp Ile  Ile Asp Glu 
    1160                 1165                 1170             


Lys Leu  Ser Lys Ile Ser Lys  Asn Gly Lys Ile Thr  His Glu Leu 
    1175                 1180                 1185             


Phe Glu  Val Val Leu Pro Arg  Val Gly Gln Lys Thr  Leu Phe Lys 
    1190                 1195                 1200             


Asn Ser  Lys Tyr Asn Leu Phe  Leu Val Asn Ser Asp  Thr Tyr Met 
    1205                 1210                 1215             


His Asn  Tyr Gln Glu Leu Trp  Met Pro Arg Glu Tyr  Gln Lys Met 
    1220                 1225                 1230             


Trp Lys  Asp Ile Ser Leu Ser  Lys Tyr Gly Asp Ala  Gln Thr Glu 
    1235                 1240                 1245             


Asp Gln  Leu Asp Gln Ile Phe  Gly Phe Ile Val Ser  Gln Val Asn 
    1250                 1255                 1260             


Ser Tyr  Phe Asn Leu Tyr Asp  Ile Asn Gln Phe Arg  Lys Lys Leu 
    1265                 1270                 1275             


Asn Asp  Ala Ala Asp Lys Phe  Ala Thr Leu Pro Met  Arg Asp Thr 
    1280                 1285                 1290             


Asp Asp  Met Gln Gly Lys Ile  Ala Thr Ile Gly Gln  Leu Leu Ile 
    1295                 1300                 1305             


Gly Leu  Gln Ala Asn Ala Ala  Arg Ser Asp Leu Arg  Asn Leu Asp 
    1310                 1315                 1320             


Ile Lys  Thr Pro Leu Gly Leu  Leu Gln Ile Pro Asn  Gly Ile Ser 
    1325                 1330                 1335             


Leu Asp  Ser Asp Thr Ser Val  Val Tyr Gln Ser Pro  Thr Gly Leu 
    1340                 1345                 1350             


Leu Glu  Arg Glu Val His Leu  Ser Asp Leu 
    1355                 1360             


<210>  967
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  967

Met Thr Lys Leu Asn Gln Pro Tyr Gly Ile Gly Leu Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Val Val Asp Ala Asn Ser His Leu Leu Arg 
            20                  25                  30          


Leu Lys Gly Glu Thr Ala Ile Gly Ala Arg Leu Phe Arg Glu Gly Gln 
        35                  40                  45              


Ser Ala Ala Asp Arg Arg Glu Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Thr Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Ala Pro His 
65                  70                  75                  80  


Ile Thr Lys Ile Asp Pro Asp Phe Phe Leu Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Asp Arg Phe Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Glu Ala Tyr Pro Ser Met Tyr 
        115                 120                 125             


His Leu Arg Leu Arg Leu Met Thr Asp Ala Ser Gln Ala Asp Pro Arg 
    130                 135                 140                 


Glu Ile Phe Leu Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Ala Ala Lys Asp Phe Asn Thr Asp Glu Val Asp Leu 
                165                 170                 175     


Lys His Ile Phe Pro Ala Leu Ala Glu Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Glu Leu Asn Phe Asp Ser Ala Leu Ala Asp Asp Phe Lys Ala 
        195                 200                 205             


Lys Leu Leu Asp Glu Asp Ala Lys Pro Ser Asp Ile Gln Lys Ala Leu 
    210                 215                 220                 


Val Asn Leu Leu Leu Ser Ser Asp Gly Glu Lys Glu Ile Val Lys Lys 
225                 230                 235                 240 


Arg Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Ile Thr Gly Leu Lys 
                245                 250                 255     


Thr Lys Phe Asn Leu Ala Leu Gly Thr Glu Val Asp Glu Ala Asp Ala 
            260                 265                 270         


Ser Asn Trp Gln Phe Ser Met Gly Gln Leu Asp Asp Lys Trp Ser Asn 
        275                 280                 285             


Ile Glu Thr Ser Met Thr Asp Gln Gly Thr Glu Ile Phe Glu Gln Ile 
    290                 295                 300                 


Gln Glu Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Met Ser Leu Ser Gln Ala Lys Val Ala Asp Tyr Gly Gln His Lys Glu 
                325                 330                 335     


Asp Leu Glu Leu Phe Lys Thr Tyr Ile Lys Lys Leu Asn Asp His Glu 
            340                 345                 350         


Leu Ala Lys Thr Ile Arg Gly Leu Tyr Asp Arg Tyr Ile Asn Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Leu Arg Glu Asp Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Asn Glu Val Ser Glu Gln Leu Leu Asn Arg 
385                 390                 395                 400 


Met Gly Gln Ala Asn Phe Met Leu Lys Gln Arg Thr Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Leu Gln Gln Arg Glu Leu Asp Gln Ile Ile Ala 
            420                 425                 430         


Asn Gln Ser Lys Tyr Tyr Asp Trp Leu Ala Lys Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Ala Ser Lys Pro Tyr Gln Leu Asp Glu Leu Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ile Thr Pro Lys Gln Gln Ala 
465                 470                 475                 480 


Glu Ser Gly Glu Asn Val Phe Ala Trp Met Val Arg Lys Asn Pro Ser 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Asp Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Lys Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Ile Asn Asn Glu Ala Leu 
545                 550                 555                 560 


Ser Thr Asp Gln Lys Gln Arg Leu Ile Arg Glu Val Phe Glu Arg His 
                565                 570                 575     


Ser Ser Val Thr Ile Lys Gln Val Ala Asp Asn Leu Val Ala His Gly 
            580                 585                 590         


Asp Phe Ala Gln Arg Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr His Gln Leu Lys Glu Ile Leu His 
    610                 615                 620                 


Gly Ala Ile Asp Asp Pro Thr Lys Leu His Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Thr Trp Ser Thr Val Phe Glu Asp Ala Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Ala Glu Ile Asp Trp Leu Asp Pro Ala Thr Ile Lys Lys Leu Ser Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Arg Lys Leu Leu Asp Gly 
        675                 680                 685             


Leu Lys Leu Gly Asn Gly His Thr Val Ile Gln Glu Leu Met Leu Ser 
    690                 695                 700                 


Asn His Asn Leu Met Gln Ile Leu Ala Asp Glu Thr Leu Lys Glu Thr 
705                 710                 715                 720 


Met Thr Glu Leu Asn Gln Asp Lys Leu Lys Thr Asp Asp Ile Glu Asp 
                725                 730                 735     


Val Ile Asn Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Arg Val Val Glu Asp Ile Lys His Ala Ala Asn Gly Gln 
        755                 760                 765             


Asp Pro Ser Trp Leu Tyr Ile Glu Thr Ala Asp Gly Pro Gly Thr Ala 
    770                 775                 780                 


Gly Lys Arg Thr Gln Ser Arg Gln Lys Gln Ile Gln Thr Val Tyr Ala 
785                 790                 795                 800 


Asn Ala Ala Gln Glu Leu Ile Asp Ser Ala Val Arg Gly Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Ala Asp Lys Ala Ser Phe Thr Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Ala Pro Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser His Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Asn Ala Thr Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Asn Val Phe Ala Ser Thr Leu Phe Ala Gly Lys 
                885                 890                 895     


Met Lys Ala Thr Trp Arg Lys Trp His Glu Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Arg Asn Leu Met Leu Arg Pro Asp Glu Ile Asp Lys Phe 
        915                 920                 925             


Ala Lys Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Ala Ala Ala Gln Tyr Pro Asn Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu Leu Asp 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Tyr Pro Lys 
        995                 1000                 1005             


Leu Thr  Pro Phe Phe Thr Tyr  Gly Glu Phe Ala Lys  Val Asp Val 
    1010                 1015                 1020             


Lys Lys  Phe Arg Glu Phe Asn  Phe Ile Gly Ala Leu  Thr His Ala 
    1025                 1030                 1035             


Lys Lys  Asn Ile Ile Ala Lys  Asp Thr Gly Glu Ile  Val Trp Asp 
    1040                 1045                 1050             


Lys Glu  Arg Asp Ile Gln Glu  Leu Asp Arg Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val His Phe Asp Met  Ala Asp Leu 
    1070                 1075                 1080             


Phe Asp  Gln Thr Ile Tyr Ala  Ala Lys Asp Ser Lys  Glu Arg Gly 
    1085                 1090                 1095             


Gly Ser  Lys Asn Leu Ile Gln  Lys Lys Gln Gly Tyr  Leu Thr Gly 
    1100                 1105                 1110             


Ile Tyr  Gly Gly Tyr Ser Thr  Glu Arg Gly Ser Tyr  Val Ser Ile 
    1115                 1120                 1125             


Ile Arg  Thr Phe Lys Lys Gly  Ala Pro Ile Tyr Asn  Ile Val Asn 
    1130                 1135                 1140             


Ile Ser  Thr Ala Asp Val Ser  Leu Leu Ser Asp Lys  Gln Arg Val 
    1145                 1150                 1155             


Ser Glu  Ala Ser Ala Asp Val  Leu Leu Lys Glu Leu  Ile Lys Lys 
    1160                 1165                 1170             


Lys Leu  Ala Lys Arg Lys Ser  Gly Glu Asn Thr Lys  Ser Gly Ala 
    1175                 1180                 1185             


Phe Glu  Ile Val Leu Pro Arg  Val Gly Lys Asn Thr  Leu Phe Lys 
    1190                 1195                 1200             


Asn Lys  Lys Tyr Gly Leu Phe  Leu Val Glu Ser Thr  Thr Glu Phe 
    1205                 1210                 1215             


His Asn  Tyr Gln Glu Leu Trp  Leu Pro Arg Asn Asp  Gln Met Leu 
    1220                 1225                 1230             


Ile Lys  Ser Ile Gln Ser His  Lys Ile Glu Glu Ser  Lys Ala Asp 
    1235                 1240                 1245             


Tyr Glu  Leu Thr Arg Met Tyr  Arg Asn Ile Ala Ser  Gln Val Asp 
    1250                 1255                 1260             


His Tyr  Phe Gly Leu Tyr Thr  Ile Arg Lys Phe Ser  Glu Lys Leu 
    1265                 1270                 1275             


Thr Ala  Ser Ile Asn Asp Tyr  Gly Ala Leu Pro Val  Tyr Lys Ser 
    1280                 1285                 1290             


Glu Thr  Thr Asp Gly Lys Tyr  Glu Val Leu Ser Asn  Leu Phe Glu 
    1295                 1300                 1305             


Gly Leu  Gly Ser Gly Thr Ser  Thr Gly Asn Leu Thr  Ala Val Lys 
    1310                 1315                 1320             


Leu Gln  Pro Asn Phe Gly Arg  Val Tyr Leu Thr Ser  Gly Ile Val 
    1325                 1330                 1335             


Leu Glu  Asp Asp Thr Ser Ile  Ile Tyr Gln Ser Pro  Thr Gly Leu 
    1340                 1345                 1350             


Phe Glu  Arg Ser Val Lys Leu  Ala Asp Leu 
    1355                 1360             


<210>  968
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  968

Met Thr Lys Leu Asn Gln Pro Tyr Gly Ile Gly Leu Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Val Val Asp Ala Asn Ser His Leu Leu Arg 
            20                  25                  30          


Leu Lys Gly Glu Thr Ala Ile Gly Ala Arg Leu Phe Arg Glu Gly Gln 
        35                  40                  45              


Ser Ala Ala Asp Arg Arg Gly Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Thr Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Ala Pro His 
65                  70                  75                  80  


Ile Thr Lys Ile Asp Pro Asp Phe Phe Leu Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Asp Arg Phe Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Glu Asp Tyr Pro Ser Met Tyr 
        115                 120                 125             


His Leu Arg Leu His Leu Met Thr His Thr His Lys Ala Asp Pro Arg 
    130                 135                 140                 


Glu Ile Phe Leu Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Ala Ala Lys Asp Phe Asn Thr Asp Lys Val Asp Leu 
                165                 170                 175     


Glu Asp Ile Phe Pro Ala Leu Thr Glu Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Glu Leu Thr Phe Asp Leu Ala Lys Ala Asp Asp Phe Lys Ala 
        195                 200                 205             


Lys Leu Leu Asp Glu Gln Ala Thr Pro Ser Asp Thr Gln Lys Ala Leu 
    210                 215                 220                 


Val Asn Leu Leu Leu Ser Ser Asp Gly Glu Lys Glu Ile Val Lys Lys 
225                 230                 235                 240 


Arg Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Ile Thr Gly Leu Lys 
                245                 250                 255     


Thr Lys Phe Asn Leu Ala Leu Gly Thr Glu Val Asp Glu Ala Asp Ala 
            260                 265                 270         


Ser Asn Trp Gln Phe Ser Met Gly Gln Leu Asp Asp Lys Trp Ser Asn 
        275                 280                 285             


Ile Glu Thr Ser Met Thr Asp Gln Gly Thr Glu Ile Phe Glu Gln Ile 
    290                 295                 300                 


Gln Glu Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Met Ser Leu Ser Gln Ala Lys Val Ala Asp Tyr Gly Gln His Lys Glu 
                325                 330                 335     


Asp Leu Glu Leu Phe Lys Thr Tyr Leu Lys Lys Leu Asn Asp His Glu 
            340                 345                 350         


Leu Ala Lys Thr Ile Arg Gly Leu Tyr Asp Arg Tyr Ile Asn Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Leu Arg Glu Asp Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Asn Glu Val Ser Glu Gln Leu Leu Asn Arg 
385                 390                 395                 400 


Met Gly Gln Ala Asn Phe Met Leu Lys Gln Arg Thr Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Ile Gln Leu Gln Gln Arg Glu Leu Asp Gln Ile Ile Ala 
            420                 425                 430         


Asn Gln Ser Lys Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro Val Glu 
        435                 440                 445             


Ala His Arg Trp Lys Met Pro Tyr Gln Leu Asp Glu Leu Leu Asn Phe 
    450                 455                 460                 


His Ile Pro Tyr Tyr Val Gly Pro Leu Ile Thr Pro Lys Gln Gln Ala 
465                 470                 475                 480 


Glu Ser Gly Glu Asn Val Phe Ala Trp Met Val Arg Lys Asp Pro Ser 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Lys Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Ile Asn Asn Glu Cys Leu 
545                 550                 555                 560 


Gly Thr Asp Gln Lys Gln Arg Leu Ile Arg Glu Val Phe Glu Arg His 
                565                 570                 575     


Ser Ser Val Thr Ile Lys Gln Val Ala Asp Asn Leu Val Ala His Gly 
            580                 585                 590         


Asp Phe Ala Arg Arg Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr His Gln Leu Lys Glu Ile Leu His 
    610                 615                 620                 


Glu Ala Ile Asp Asp Pro Thr Lys Leu Leu Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Thr Trp Ser Thr Val Phe Glu Asp His Thr Ile Phe Glu Thr Lys Leu 
                645                 650                 655     


Ala Glu Ile Glu Trp Leu Asp Pro Lys Lys Ile Asn Glu Leu Ser Gly 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Arg Lys Leu Leu Asp Gly 
        675                 680                 685             


Leu Lys Leu Gly Asn Gly His Thr Val Ile Gln Glu Leu Met Leu Ser 
    690                 695                 700                 


Asn His Asn Leu Met Gln Ile Leu Ala Asp Glu Thr Leu Lys Glu Thr 
705                 710                 715                 720 


Met Thr Glu Leu Asn Gln Asp Lys Leu Lys Thr Asp Asp Ile Glu Asp 
                725                 730                 735     


Val Ile Asn Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Arg Val Val Glu Asp Ile Lys His Ala Ala Asn Gly Gln 
        755                 760                 765             


Asp Pro Ser Trp Leu Phe Ile Glu Thr Ala Asp Gly Thr Gly Thr Ala 
    770                 775                 780                 


Gly Lys Arg Thr Gln Ser Arg Gln Lys Gln Ile Gln Thr Val Tyr Ala 
785                 790                 795                 800 


Asn Ala Ala Gln Glu Leu Ile Asp Ser Ala Val Arg Gly Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Ala Asp Lys Ala Ser Phe Thr Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Ala Pro Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser His Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Asn Ala Thr Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Asn Val Phe Ala Ser Thr Leu Phe Ala Gly Lys 
                885                 890                 895     


Met Lys Ala Thr Trp Arg Lys Trp His Glu Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Arg Asn Leu Met Leu Arg Pro Asp Glu Ile Asp Lys Phe 
        915                 920                 925             


Ala Lys Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Ala Ala Ala Gln Tyr Pro Asn Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu Leu Asp 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Tyr Pro Lys 
        995                 1000                 1005             


Leu Ala  Pro Phe Phe Thr Tyr  Gly Glu Phe Ala Lys  Val Asp Val 
    1010                 1015                 1020             


Lys Lys  Phe Arg Glu Phe Asn  Phe Ile Gly Ala Leu  Thr His Ala 
    1025                 1030                 1035             


Lys Lys  Asn Ile Ile Ala Lys  Asp Thr Gly Glu Ile  Val Trp Asp 
    1040                 1045                 1050             


Lys Glu  Arg Asp Ile Arg Glu  Leu Asp Arg Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val Tyr Phe Glu Thr  Ala Asp Leu 
    1070                 1075                 1080             


Phe Lys  Gln Thr Ile Tyr Ala  Ala Lys Asp Ser Lys  Glu Arg Gly 
    1085                 1090                 1095             


Gly Ser  Lys Gln Leu Ile Pro  Lys Lys Gln Gly Tyr  Pro Thr Gln 
    1100                 1105                 1110             


Val Tyr  Gly Gly Tyr Thr Gln  Glu Ser Gly Ser Tyr  Asn Ala Leu 
    1115                 1120                 1125             


Val Arg  Val Ala Glu Ala Asp  Thr Thr Ala Tyr Gln  Val Ile Lys 
    1130                 1135                 1140             


Ile Ser  Ala Gln Asn Ala Ser  Lys Ile Ala Ser Ala  Asn Leu Lys 
    1145                 1150                 1155             


Ser Arg  Glu Lys Gly Lys Gln  Leu Leu Asn Glu Ile  Val Val Lys 
    1160                 1165                 1170             


Gln Leu  Ala Lys Arg Arg Lys  Asn Trp Lys Pro Ser  Ala Asn Ser 
    1175                 1180                 1185             


Phe Lys  Ile Val Ile Pro Arg  Phe Gly Met Gly Thr  Leu Phe Gln 
    1190                 1195                 1200             


Asn Ala  Lys Tyr Gly Leu Phe  Met Val Asn Ser Asp  Thr Tyr Tyr 
    1205                 1210                 1215             


Arg Asn  Tyr Gln Glu Leu Trp  Leu Ser Arg Glu Asn  Gln Lys Leu 
    1220                 1225                 1230             


Leu Lys  Lys Leu Phe Ser Ile  Lys Tyr Glu Lys Thr  Gln Met Asn 
    1235                 1240                 1245             


His Asp  Ala Leu Gln Val Tyr  Lys Ala Ile Ile Asp  Gln Val Glu 
    1250                 1255                 1260             


Lys Phe  Phe Lys Leu Tyr Asp  Ile Asn Gln Phe Arg  Ala Lys Leu 
    1265                 1270                 1275             


Ser Asp  Ala Ile Glu Arg Phe  Glu Lys Leu Pro Ile  Asn Thr Asp 
    1280                 1285                 1290             


Gly Asn  Lys Ile Gly Lys Thr  Glu Thr Leu Arg Gln  Ile Leu Ile 
    1295                 1300                 1305             


Gly Leu  Gln Ala Asn Gly Thr  Arg Ser Asn Val Lys  Asn Leu Gly 
    1310                 1315                 1320             


Ile Lys  Thr Asp Leu Gly Leu  Leu Gln Val Gly Ser  Gly Ile Lys 
    1325                 1330                 1335             


Leu Asp  Lys Asp Thr Gln Ile  Val Tyr Gln Ser Pro  Ser Gly Leu 
    1340                 1345                 1350             


Phe Lys  Arg Arg Ile Pro Leu  Ala Asp Leu 
    1355                 1360             


<210>  969
<211>  1364
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  969

Met Gly Asp Arg Lys Tyr Asn Leu Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Phe Ala Ala Val Asp Glu Asn Asn Gln Pro Ile Arg Val Lys 
            20                  25                  30          


Gly Lys Thr Ala Ile Gly Val Arg Leu Phe Glu Glu Gly Lys Thr Ala 
        35                  40                  45              


Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Arg Trp Arg Ile Asn Leu Leu Asn Glu Ile Phe Asp Ala His Leu Ala 
65                  70                  75                  80  


Glu Val Asp Pro Thr Phe Leu Ala Arg Leu Lys Glu Ser Asn Arg Ser 
                85                  90                  95      


Asn Leu Asp Pro Lys Lys Ser Phe Gln Gly Ser Leu Leu Phe Pro Glu 
            100                 105                 110         


Arg Lys Asp Tyr Gln Phe Tyr Glu Glu Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Lys Ala Leu Met Glu Lys Asp Arg Lys Phe Asp Phe Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Val His His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Asn 
145                 150                 155                 160 


Gly Thr Pro Met Arg Ser Phe Lys Val Glu Asn Ile Glu Leu Asp Thr 
                165                 170                 175     


Leu Phe Asp Gln Leu Asn Gln Leu Tyr Ala Glu Ile Val Pro Asp Asn 
            180                 185                 190         


Glu Leu Ala Phe Asp Leu Ala Gln Val Ala Asp Val Lys Asp Val Leu 
        195                 200                 205             


Ser Ser Thr Thr Ile Tyr Lys Met Asp Lys Lys Lys Gln Leu Val Lys 
    210                 215                 220                 


Met Met Leu Leu Pro Ala Ser Asn Lys Ala Leu Gln Ser Glu Asn Lys 
225                 230                 235                 240 


Lys Ile Val Thr Gln Phe Val Asn Ala Ile Leu Asn Tyr Lys Phe Lys 
                245                 250                 255     


Leu Asp Val Leu Leu Gln Val Glu Thr Asp Ala Asp Trp Ser Leu Lys 
            260                 265                 270         


Leu Asn Asp Glu Gly Ala Asp Asp Lys Leu Glu Glu Phe Thr Gly Asp 
        275                 280                 285             


Leu Asp Glu Asn Arg Leu Glu Ile Ile Asp Leu Leu Gln Arg Leu His 
    290                 295                 300                 


Asn Trp Phe Ser Leu Asn Glu Ile Thr Lys Asp Gly Asn Ser Leu Ser 
305                 310                 315                 320 


Ala Ala Met Val Glu Lys Tyr Glu Asn His His His His Leu Gly Leu 
                325                 330                 335     


Leu Lys Lys Val Ile Glu Asn His Pro Asp Ala Lys Lys Ala Lys Ala 
            340                 345                 350         


Leu Lys Glu Thr Tyr Thr Ala Tyr Val Gly Lys Thr Asp Asp Lys Thr 
        355                 360                 365             


Gln Asn Gln Asp Asp Phe Tyr Lys Ala Val Glu Lys Asn Leu Asp Asp 
    370                 375                 380                 


Ser Pro Asp Ala Lys Glu Ile Lys Arg Leu Ile Gln Leu Asp Gln Phe 
385                 390                 395                 400 


Met Pro Lys Gln Arg Thr Gly Gln Asn Gly Ala Ile Pro His Gln Leu 
                405                 410                 415     


His Gln Gln Glu Leu Asp Gln Ile Ile Glu Lys Gln Ser Lys Tyr Tyr 
            420                 425                 430         


Pro Phe Leu Ala Glu Pro Asn Pro Asn Val Lys Arg Arg Lys Asp Ala 
        435                 440                 445             


Pro Tyr Lys Leu Asp Glu Leu Ile Ala Phe Lys Ile Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Leu Val Thr Pro Glu Glu Gln Ala Gln Asn Gly Glu Asn Val 
465                 470                 475                 480 


Phe Ala Trp Met Lys Arg Lys Ala Ala Gly Pro Ile Thr Pro Trp Asn 
                485                 490                 495     


Phe Asp Glu Lys Val Asp Arg Met Glu Ser Ala Asn Arg Phe Ile Arg 
            500                 505                 510         


Arg Met Thr Thr Lys Asp Thr Tyr Leu Phe Gly Glu Asp Val Leu Pro 
        515                 520                 525             


Ala Glu Ser Met Ile Tyr Gln Lys Phe Val Val Leu Asn Glu Leu Asn 
    530                 535                 540                 


Asn Leu Lys Ile Asn Gly Arg His Leu Ser Leu Lys Asp Lys Gln Asp 
545                 550                 555                 560 


Val Tyr Asn Asp Leu Phe Lys Gln Gln Lys Thr Val Ser Ile Lys Ala 
                565                 570                 575     


Leu Gln Asn Tyr Tyr Val Thr Lys Lys Lys Ala Ala Thr Ala Pro Thr 
            580                 585                 590         


Val Gly Gly Leu Ala Asp Pro Lys Lys Phe Leu Ser Ser Leu Ser Thr 
        595                 600                 605             


Tyr Ile Asp Phe Lys Asn Met Phe Gly Glu Arg Val Asn Asp Pro Gln 
    610                 615                 620                 


Phe Gln Glu Asp Leu Glu Gln Ile Val Glu Trp Ser Thr Ile Phe Glu 
625                 630                 635                 640 


Asp Arg Gly Ile Phe Lys Ala Lys Leu Gln Ala Leu Gly Trp Leu Ser 
                645                 650                 655     


Glu Lys Gln Ile Gln Gln Leu Val Ala Lys Arg Tyr Lys Gly Trp Gly 
            660                 665                 670         


Arg Leu Ser Lys Lys Leu Leu Thr Gly Leu Lys Asn Ala Glu Gly Tyr 
        675                 680                 685             


Ser Ile Leu Asp Glu Met Trp Arg Ser Thr Gly Asn Phe Met Gln Ile 
    690                 695                 700                 


Gln Ser Arg Pro Glu Phe Ala Ala Leu Ile Gln Gln Ala Asn Glu Lys 
705                 710                 715                 720 


Gln Phe Glu Gly Asn Asp Pro Asp Asn Val Trp Glu Asn Ile Glu Asn 
                725                 730                 735     


Ile Leu Gly Asp Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Val Val Lys Val Val Gln Asp Ile Glu Lys Ala Val Gly Asn Pro 
        755                 760                 765             


Pro Glu Lys Ile Ala Ile Glu Phe Thr Arg Glu Ala Ala Ala Asn Pro 
    770                 775                 780                 


Gln Arg Thr Gln Ser Arg Leu Arg Thr Leu Glu Lys Leu Tyr Glu Ser 
785                 790                 795                 800 


Ala Glu Glu Val Val Asp Ala Gly Leu Thr Ala Glu Leu Ala Glu Phe 
                805                 810                 815     


Lys Glu Asn Lys His Val Leu Ser Asp Lys Tyr Tyr Leu Tyr Phe Thr 
            820                 825                 830         


Gln Leu Gly Arg Asp Val Tyr Thr Gly Asp Thr Ile Ser Leu Asp Lys 
        835                 840                 845             


Leu Asn Asp Tyr Asp Val Asp His Ile Leu Pro Gln Ser Phe Ile Lys 
    850                 855                 860                 


Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ile Arg Ala Val Asn Asn 
865                 870                 875                 880 


Gly Lys Ser Asp Asn Val Pro Ala Lys Met Phe Gly Lys Lys Met Gly 
                885                 890                 895     


Ser Phe Trp Arg Tyr Leu Leu Asp Asn Gly Met Ile Ser Lys Arg Lys 
            900                 905                 910         


Tyr Asn Asn Leu Ile Thr Asp Pro Asp Asn Ile Ser Lys Tyr Ala Gln 
        915                 920                 925             


Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys 
    930                 935                 940                 


Leu Thr Ala Asn Ile Leu Asn Gly Ile Tyr Asp Lys Asp Thr Glu Ile 
945                 950                 955                 960 


Ile Glu Val Pro Ala Lys Met Asn Ser Gln Met Arg Lys Met Phe Asp 
                965                 970                 975     


Leu Val Lys Val Arg Glu Val Asn Asp Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Tyr Leu Thr Ile Phe Ile Gly Asn  Tyr Leu Tyr Lys Cys  Tyr Pro Lys 
        995                 1000                 1005             


Leu Gln  Pro Tyr Phe Val Tyr  Asp Asn Phe Lys Lys  Phe Gly Asn 
    1010                 1015                 1020             


Lys Glu  Asp Ile Gly His Lys  Arg Phe Asn Phe Leu  Gly Lys Ile 
    1025                 1030                 1035             


Glu Arg  Glu Lys Lys Val Val  Ala Pro Glu Thr Gly  Glu Ile Leu 
    1040                 1045                 1050             


Trp Ser  Asn Val Ala Pro Asn  Glu Thr Ile Lys Gln  Ile Lys Lys 
    1055                 1060                 1065             


Val Tyr  Asp Tyr Lys Phe Met  Ile Val Ser Arg Glu  Ile Thr Thr 
    1070                 1075                 1080             


Arg Arg  Ala Glu Leu Phe Asn  Gln Thr Val Tyr Pro  Lys Asn Tyr 
    1085                 1090                 1095             


His Gly  Lys Leu Ile Pro Ile  Lys Glu Asp Arg Pro  Thr Asp Leu 
    1100                 1105                 1110             


Tyr Gly  Gly Tyr Ser Gly Asn  Thr Asp Ala Tyr Leu  Ala Ile Val 
    1115                 1120                 1125             


Ala Leu  Glu Asp Lys Lys Lys  Gly Lys Tyr Phe Lys  Val Val Gly 
    1130                 1135                 1140             


Ile Pro  Thr Arg Val Ala Ala  Lys Leu Glu Lys Leu  Lys Gln Gln 
    1145                 1150                 1155             


Asp Ser  Gln Gln Tyr Leu Gln  Ala Leu His Lys Val  Ile Ala Pro 
    1160                 1165                 1170             


Gln Phe  Thr Lys Ser Thr Lys  Lys Gly Ile Lys Lys  Thr Glu Phe 
    1175                 1180                 1185             


Glu Ile  Val Leu Asp Lys Val  His Tyr Arg Gln Leu  Val Gln Asp 
    1190                 1195                 1200             


Gly Pro  Val Lys Met Met Leu  Gly Ser Ser Thr Tyr  Lys Tyr Asn 
    1205                 1210                 1215             


Ala Lys  Gln Leu Val Leu Ser  Glu Lys Ala Leu Gln  Val Ile Ala 
    1220                 1225                 1230             


Asp Asp  Arg Lys Phe Asp Glu  Thr Gln Lys Asp Asp  Asn Leu Ile 
    1235                 1240                 1245             


Ala Val  Tyr Asp Glu Ile Leu  Ser Ile Val Asn Gln  Ser Phe Asp 
    1250                 1255                 1260             


Leu Tyr  Asp Ile Asn Gly Phe  Arg Lys Lys Leu Asn  Asp Asn Arg 
    1265                 1270                 1275             


Asp Gln  Phe Ile Asp Leu Pro  Ala Glu Thr Lys Tyr  Glu Gly Arg 
    1280                 1285                 1290             


Lys Val  Val Ala His Gly Lys  Arg Glu Met Ile Leu  Glu Ile Leu 
    1295                 1300                 1305             


Lys Gly  Leu His Ala Asn Ala  Ala Phe Gly Asn Leu  Lys Pro Ile 
    1310                 1315                 1320             


Gly Phe  Ser Thr Ala Phe Gly  Gln Leu Gln Val Pro  Asn Gly Ile 
    1325                 1330                 1335             


Ile Leu  Ser Lys Asn Ala Ile  Leu Ile His Gln Ser  Pro Ser Gly 
    1340                 1345                 1350             


Leu Phe  Glu Arg Lys Ile Lys  Leu Ser Asp Leu 
    1355                 1360                 


<210>  970
<211>  1364
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  970

Met Glu Lys Gln Glu Tyr Tyr Leu Gly Leu Asp Met Gly Ile Ser Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Thr Asn Asp Lys Tyr Glu Leu Leu Arg Lys Lys 
            20                  25                  30          


Gly Lys Asp Met Trp Gly Ile Arg Glu Phe Glu Glu Ala Asp Ser Ser 
        35                  40                  45              


Ala Glu Arg Arg Thr His Arg Val Asn Arg Arg Arg Arg Gln Arg Gln 
    50                  55                  60                  


Val Ala Arg Met Gly Leu Leu Lys Glu Tyr Phe Ser Asp Ala Ile Glu 
65                  70                  75                  80  


Lys Val Asp Ser Asn Phe Phe Ile Arg Ile Glu Asn Ser Lys Tyr Phe 
                85                  90                  95      


Phe Glu Asp Lys Asp Gly Arg Leu Ser Ser Lys Asn Gly Ile Phe Asp 
            100                 105                 110         


Asp Ala Asp Tyr Lys Asp Val Asp Tyr Tyr Thr Glu Tyr Pro Thr Ile 
        115                 120                 125             


Phe His Leu Arg Lys Ala Leu Leu Asp Ile Glu Asp Thr Gly Lys Glu 
    130                 135                 140                 


Tyr Asp Val Arg Leu Val Tyr Leu Ala Leu Val Asn Met Phe Lys His 
145                 150                 155                 160 


Arg Gly His Phe Leu Leu Asn Thr Glu Gly Asp Ala Leu Ser Ser Glu 
                165                 170                 175     


Gln Val Asn Thr Thr Tyr Gln Glu Leu Leu Glu Ile Leu Glu Asn Asn 
            180                 185                 190         


Tyr Asp Ile Ser Leu Glu Ser Arg Asp Ile Lys Asp Val Ile Asp Ile 
        195                 200                 205             


Ile Ser Ser Ser Asp Asn Gly Arg Lys Lys Lys His Glu Gln Ile Leu 
    210                 215                 220                 


Glu Leu Phe Gly Val Lys Lys Ser Asp Lys Arg Ala Asn Glu Leu Leu 
225                 230                 235                 240 


Lys Cys Leu Cys Gly Leu Ser Val Asn Ala Lys Leu Ile Phe Asp Glu 
                245                 250                 255     


Asn Tyr Glu Glu Lys Val Asp Leu Cys Phe His Asp Phe Ser Tyr Thr 
            260                 265                 270         


Asp Lys Ile Pro Asp Leu Met Glu Lys Leu Ser Glu Glu Glu Tyr Tyr 
        275                 280                 285             


Val Val Glu Gln Leu Lys Ser Ile Tyr Asp Tyr Ser Ile Leu Ala Gly 
    290                 295                 300                 


Thr Leu Lys Gly Tyr Asp Tyr Leu Ser Cys Ala Arg Val Glu Leu Tyr 
305                 310                 315                 320 


Glu Lys His Arg Glu Asp Leu Lys Leu Leu Lys Ala Leu Tyr Arg Arg 
                325                 330                 335     


Tyr Lys Thr Asn Glu Glu Tyr Asn Asp Met Phe Lys Ser Asp Lys Glu 
            340                 345                 350         


Gly Thr Tyr Ser Ala Tyr Val Asn Ser Phe Asn Ser Ser Asp Ser Ser 
        355                 360                 365             


Val Lys Ile Lys Lys Tyr Arg Trp Asn Met Lys Gly Arg Thr Ile Asp 
    370                 375                 380                 


Asn Leu Tyr Gly Thr Ile Lys Lys Ala Phe Lys Asp Asn Thr Asp Asp 
385                 390                 395                 400 


Lys Asp Val Gln Ser Met Met Glu Ser Ile Asp Lys Glu Gln Phe Leu 
                405                 410                 415     


Pro Lys Gln Leu Thr Gly Ala Asn Gly Val Ile Pro Asn Gln Val His 
            420                 425                 430         


Lys Lys Glu Met Glu Arg Ile Leu Lys Lys Ala Gln Asn Tyr Leu Pro 
        435                 440                 445             


Phe Leu Lys Glu Lys Asp Glu Ser Glu Leu Thr Val Ser Glu Arg Ile 
    450                 455                 460                 


Leu Arg Leu Phe Thr Phe Gln Ile Pro Tyr Tyr Val Gly Pro Leu Gly 
465                 470                 475                 480 


Glu Asn Ser Lys Thr Gly Trp Val Val Arg Arg Glu Gly Gly Arg Val 
                485                 490                 495     


Leu Pro Trp Asn Ile Asp Glu Lys Ile Asp Met Ser Ala Thr Ser Glu 
            500                 505                 510         


Ala Phe Ile Gly Asn Leu Ile Arg Lys Cys Thr Tyr Leu Ser Asp Glu 
        515                 520                 525             


Lys Val Met Pro Lys Ala Ser Leu Ser Tyr Glu Arg Tyr Cys Val Leu 
    530                 535                 540                 


Asn Glu Ile Asn Asn Ile Cys Ile Asp Gly Glu Arg Leu Asp Thr Glu 
545                 550                 555                 560 


Leu Lys Gln Lys Ile Tyr Thr Glu Cys Phe Met Lys Gly Lys Arg Val 
                565                 570                 575     


Thr Arg Lys Gln Ile Cys Asn Tyr Leu Val Ala Gln Gly Cys Ile Lys 
            580                 585                 590         


Ser Asp Glu Gln Val Thr Gly Ile Asp Ile Asn Val Asn Ser Ser Leu 
        595                 600                 605             


Ser Ser Tyr Gly Lys Met Phe Ala Ile Phe Gly Glu His Leu Lys Glu 
    610                 615                 620                 


Asp Ala Ile Arg Lys Val Ala Glu Glu Ile Ile Tyr Trp Gly Thr Ile 
625                 630                 635                 640 


Phe Ser Asp Ser Lys Lys Met Phe Arg Lys His Leu Thr Lys Tyr Val 
                645                 650                 655     


Glu Gln Gly Ile Leu Gly Glu Ser Gln Val Lys Arg Ile Leu Gly Tyr 
            660                 665                 670         


Lys Phe Lys Asp Trp Gly Arg Phe Ser Lys Ala Leu Leu Thr Leu Gln 
        675                 680                 685             


Gly Ile Asp Lys Ser Thr Gly Glu Leu Leu Ser Leu Glu Asn Ala Met 
    690                 695                 700                 


Trp Glu Tyr Ser Leu Asn Phe Met Glu Leu Ile Asn Ser Asp Asp Phe 
705                 710                 715                 720 


Thr Phe Lys Asp Glu Leu Ala Asn Arg Arg Thr Ser Ala Ile Lys Ser 
                725                 730                 735     


Leu Ser Glu Phe Thr Tyr Glu Asp Leu Glu Asp Thr Tyr Phe Ser Val 
            740                 745                 750         


Pro Val Lys Arg Met Ile Trp Gln Thr Ile Leu Val Val Arg Glu Ile 
        755                 760                 765             


Glu Gln Ile Met Gly Tyr Ala Pro Lys Lys Val Phe Val Glu Met Ala 
    770                 775                 780                 


Arg Ser Glu Glu Glu Lys Gly Asp Lys Gly Arg Lys Asp Ser Arg Gly 
785                 790                 795                 800 


Thr Gln Leu Leu Glu Leu Tyr Lys Asn Ile Lys Asn Thr Glu Asn His 
                805                 810                 815     


Asp Trp Lys Lys Glu Ile Ser Glu Ala Asn Glu Ser Gly Lys Leu Arg 
            820                 825                 830         


Ser Lys Lys Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Asp Met Tyr 
        835                 840                 845             


Thr Gly Glu Glu Ile Asn Ile Asp Met Leu Tyr Asp Asp Ser Met Tyr 
    850                 855                 860                 


Asp Ile Asp His Ile Tyr Pro Arg His Tyr Val Lys Asp Asp Ser Ile 
865                 870                 875                 880 


Val Asn Asn Leu Val Leu Val Asn Arg Arg Ala Asn Gln Asp Ile Lys 
                885                 890                 895     


Lys Asp Leu Tyr Pro Ile Pro Asp Lys Ile Ile Ser Asn Pro Lys Val 
            900                 905                 910         


Gln Glu Leu Trp Lys Met Leu His Lys Gln Asn Leu Ile Thr Asp Glu 
        915                 920                 925             


Lys Tyr Lys Arg Leu Met Ser Arg Ser Pro Phe Thr Glu Lys Gln Leu 
    930                 935                 940                 


Gly Asp Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Gly Thr Lys 
945                 950                 955                 960 


Gly Ile Ala Glu Leu Leu Gly Gln Leu Phe Lys Asp Thr Glu Ile Val 
                965                 970                 975     


Tyr Ala Lys Ala Thr Asn Val Ser Asp Phe Arg Arg Asp Asn Gly Phe 
            980                 985                 990         


Val Lys Ser Arg Leu Val Asn Asp  Phe His His Ala Gln  Asp Ala Tyr 
        995                 1000                 1005             


Leu Asn  Ile Val Val Gly Asn  Val Tyr Tyr Thr Lys  Phe Thr Lys 
    1010                 1015                 1020             


Asn Pro  Trp Asn Phe Ile Lys  Lys Asp Phe Asp Lys  Asp Arg Asn 
    1025                 1030                 1035             


Lys Asn  Gly Tyr Asn Leu Ala  Lys Met Phe Asp Arg  Asp Val Val 
    1040                 1045                 1050             


Arg Gly  Asp Asp Thr Ala Trp  Ile Ala Ser Lys Lys  Asp Thr Gln 
    1055                 1060                 1065             


Gly Thr  Ile Glu Thr Val Arg  Lys Met Met Arg Lys  Asn Thr Pro 
    1070                 1075                 1080             


Leu Met  Thr Arg Met Ser Phe  Glu Gln His Gly Gly  Ile Ala Asn 
    1085                 1090                 1095             


Ala Thr  Leu Tyr Ser Ala Lys  Lys Ala Lys Val Glu  Asn Tyr Ile 
    1100                 1105                 1110             


Pro Leu  Lys Ser Ser Asp Ile  Arg Leu Ser Asp Val  Lys Lys Tyr 
    1115                 1120                 1125             


Gly Gly  Phe Thr Ser Ala Thr  Val Ala Tyr Tyr Phe  Ile Val His 
    1130                 1135                 1140             


His Lys  Glu Gly Lys Lys Glu  Asn Thr Thr Ile Glu  Ala Leu Pro 
    1145                 1150                 1155             


Leu Tyr  Cys Lys Arg Lys Val  Glu Asn Glu Glu Asn  Gly Leu Leu 
    1160                 1165                 1170             


Lys Tyr  Cys Glu Glu Val Leu  Gly Tyr Gly Asp Val  Lys Ile Leu 
    1175                 1180                 1185             


Phe Asn  Lys Leu Lys Ile Gln  Ser Leu Leu Asp Ile  Asn Gly Tyr 
    1190                 1195                 1200             


Gln Ala  His Leu Ser Gly Lys  Thr Gly Asn Gln Leu  Thr Leu Arg 
    1205                 1210                 1215             


Asn Ala  Met Asn Leu Cys Val  Pro Glu Lys Trp Thr  Ala Tyr Ile 
    1220                 1225                 1230             


Lys Lys  Leu Glu Lys Leu Val  Val Gly His Val Lys  Glu Gln Glu 
    1235                 1240                 1245             


Ile Ser  Ser Glu Ala Asn Ile  Glu Leu Tyr Asp Leu  Leu Thr Asp 
    1250                 1255                 1260             


Lys His  Leu Asn Ser Val Tyr  Ala Lys Arg Pro Asn  Ala Tyr Gly 
    1265                 1270                 1275             


Gln Lys  Leu Ile Asp Asn Arg  Ser Arg Phe Val Asn  Leu Lys Pro 
    1280                 1285                 1290             


Glu Ser  Gln Cys Glu Ala Ile  Leu Gln Ile Leu Lys  Leu Thr Lys 
    1295                 1300                 1305             


Ile Gly  Asn Asn Glu Thr Asn  Leu Lys Leu Ile Gly  Glu Ser Glu 
    1310                 1315                 1320             


His Ser  Gly Lys Met Leu Ile  Ser Lys Asn Leu Lys  Asp Thr Asp 
    1325                 1330                 1335             


Lys Pro  Ala Ile Ile Cys Gln  Ser Val Thr Gly Leu  Tyr Glu Arg 
    1340                 1345                 1350             


Lys Ile  Tyr Ile Phe Asp Glu  Gln Leu Asp Lys 
    1355                 1360                 


<210>  971
<211>  1364
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  971

Met Asn Lys Arg Tyr Asn Ile Gly Leu Asp Ile Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Trp Ser Val Val Asn Asp Gln Ser Gln Leu Val Arg Ile Lys Gly 
            20                  25                  30          


Lys Leu Gly Tyr Gly Val Arg Leu Tyr Asp Glu Gly Gln Thr Ala Glu 
        35                  40                  45              


Asp Arg Arg Leu Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Gly Leu Leu Arg Glu Ile Phe Glu Pro Tyr Ile Thr Pro 
65                  70                  75                  80  


Gln Asp Ala Thr Phe Phe Leu Arg Gln Lys Gln Ser Asn Leu Ser Pro 
                85                  90                  95      


Lys Asp Leu Gln Lys Gln Gln Pro Gln Ile Gly Leu Phe Asn Asp Arg 
            100                 105                 110         


Leu Asp Ser Asp Phe His Tyr Gln Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Tyr Ala Leu Met Thr Glu Lys Arg Gln Phe Asp Leu Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Met His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn Ala 
145                 150                 155                 160 


Ala Ala Ala Ser Ser Phe Lys Ser Gly Glu Ile Asp Leu Ala Lys Asp 
                165                 170                 175     


Phe Ala Ala Leu Asn Gln Ile Phe Gly Thr Ile Phe Ser Glu Val Gly 
            180                 185                 190         


Phe Ser Leu Ser Glu Ser Asn Leu Asp Glu Ile Lys Ser Ile Leu Leu 
        195                 200                 205             


Asp Asn Asn Leu Ser Ala Ser Val Arg Gln Arg Lys Ala Leu Pro Leu 
    210                 215                 220                 


Ile Tyr Gln Thr Ala Ala Ser Lys Asp Ser Glu Lys Gln Tyr Lys Ala 
225                 230                 235                 240 


Ile Ala Thr Glu Leu Leu Lys Ala Ile Leu Gly Leu Lys Ala Lys Phe 
                245                 250                 255     


Asn Leu Ile Thr Gly Ile Glu Val Glu Asp Val Lys Ala Trp Thr Leu 
            260                 265                 270         


Ser Phe Asn Ala Glu Asp Phe Asp Ala Lys Leu Ser Asp Leu Asn Ile 
        275                 280                 285             


Ala Asp Asn Asp Asp Ala Val Gln Ile Ile Glu Ile Leu Arg Ala Leu 
    290                 295                 300                 


Tyr Ser Gly Val Leu Leu Thr Gly Ile Val Pro Glu Gly Gln Ser Ile 
305                 310                 315                 320 


Ser Gln Ala Met Ile Asp Lys Tyr Glu Ala His Lys Ala His Leu Ile 
                325                 330                 335     


Met Leu Arg Ser Ile Arg Glu Gln Leu Ser Asp Lys Glu Asn Lys Ala 
            340                 345                 350         


Leu Glu Lys Ala Tyr Asp Asp Tyr Ile Asp His Pro Glu Asp Lys Gln 
        355                 360                 365             


His Gly Ser Arg Glu Asp Phe Tyr Ala Ala Ile Thr Lys Val Leu Lys 
    370                 375                 380                 


Lys Asp Ser Glu Asp Thr Asn Ile Ile Glu Ile Leu Lys Leu Ile Glu 
385                 390                 395                 400 


Leu Asp Gln Phe Met Pro Lys Gln Arg Thr Lys Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Gln Gln Glu Leu Asp Gln Ile Ile Glu Asn Gln 
            420                 425                 430         


Lys Gln Tyr Tyr Pro Trp Leu Ala Asp Ile Asn Pro Asn Glu Gln Arg 
        435                 440                 445             


Gln Lys Met Ala Pro Tyr Lys Leu Asp Glu Leu Val Thr Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Met Ile Thr Ala Glu Glu Gln Phe Gln Thr 
465                 470                 475                 480 


Ser Asn Lys Lys Phe Ala Trp Met Ile Arg Lys Glu Pro Gly Ala Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Gln Ala Ser Ala Asn 
            500                 505                 510         


Glu Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu Ala Glu 
        515                 520                 525             


Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Arg Phe Glu Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Gly Ile Lys Ile Asp Asp Gln Pro Ile Thr Val Glu 
545                 550                 555                 560 


Gln Lys Gln Ala Ile Phe Asn Asp Lys Phe Met Gln Gln Ala His Val 
                565                 570                 575     


Thr Val Lys Asn Ile Gln Ala Tyr Leu Val Ser Glu Gly Gln Tyr Ala 
            580                 585                 590         


Ser Arg Pro Asn Ile Thr Gly Leu Ser Asp Gly Glu Lys Phe Asn Ser 
        595                 600                 605             


Ser Leu Ser Thr Tyr His Asp Leu Lys Lys Ile Phe Gly Asp Met Ile 
    610                 615                 620                 


Asp Asp Pro Asn Lys Gln Thr Asp Phe Glu Lys Met Ile Glu Trp Ser 
625                 630                 635                 640 


Thr Ile Phe Glu Asp Gly Lys Ile Phe Ala Asp Lys Leu Arg Glu Ile 
                645                 650                 655     


Asn Trp Leu Thr Asp Glu Gln Arg Gln Arg Leu Ser Asn Lys Arg Tyr 
            660                 665                 670         


Arg Gly Trp Gly Gln Leu Ser Thr Lys Leu Leu Thr Lys Ile Val Asp 
        675                 680                 685             


Gln Asn Gly Gln Arg Ile Ile Asp Ile Leu Trp Asn Thr Asn Asp Asn 
    690                 695                 700                 


Phe Met Arg Ile Ile His Ser Asp Asp Phe Asp Gln Ala Val Thr Gln 
705                 710                 715                 720 


Ala Asn Gln Met Leu Leu Ala Asp Asn Asp Thr Gln Asp Val Ile Asn 
                725                 730                 735     


Glu Leu Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg Gln Ile Leu 
            740                 745                 750         


Leu Val Val Ala Asp Ile Gln Lys Ala Met Lys Gly Gln Ala Pro Ala 
        755                 760                 765             


Arg Ile Leu Ile Glu Phe Ala Arg Glu Lys Gln Ala Asn Pro Arg Leu 
    770                 775                 780                 


Ser Thr Gln Arg Arg Arg Gln Val Glu Gln Val Tyr Gln Glu Leu Asn 
785                 790                 795                 800 


Asn Asp Gln Leu Asn Lys Glu Leu Ala Asp Pro Gln Tyr Ala Asn Leu 
                805                 810                 815     


Ser Lys Asp Arg Leu Phe Leu Tyr Phe Met Gln Gly Gly Arg Asp Met 
            820                 825                 830         


Tyr Thr Gly Glu Ser Leu Asn Ile Asp Arg Leu Ser Glu Tyr Asp Ile 
        835                 840                 845             


Asp His Ile Leu Pro Gln Ala Phe Ile Lys Asp Asn Ser Leu Asp Asn 
    850                 855                 860                 


Arg Val Leu Val Ser Ala Lys Met Asn Arg Ser Lys Ser Asp His Val 
865                 870                 875                 880 


Pro Leu Asp Phe Thr Glu Glu Arg Phe Gly Gln Lys Met Arg Ser Gln 
                885                 890                 895     


Trp Glu Gln Met Leu Arg Ala Gly Leu Leu Thr Arg Arg Lys Tyr Asn 
            900                 905                 910         


Asn Leu Thr Leu Asn Pro Asp Ser Leu Asn Lys Tyr Thr Met Ser Gly 
        915                 920                 925             


Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val Ile Lys Leu Ala 
    930                 935                 940                 


Thr Asn Leu Leu Val Asp Gln Tyr Ala Asp Glu Asp Val Gln Leu Val 
945                 950                 955                 960 


Thr Val Lys Ser Gly Leu Thr His Gln Met Arg Glu Asp Phe Asp Phe 
                965                 970                 975     


Pro Lys Asn Arg Asn Leu Asn Asn Tyr His His Ala Phe Asp Ala Tyr 
            980                 985                 990         


Leu Thr Ala Phe Val Gly Leu Tyr  Leu Leu Lys Lys Tyr  Pro Lys Leu 
        995                 1000                 1005             


Ala His  Tyr Phe Val Tyr Gly  Gln Tyr Gln Gln Gly  Phe Lys Ala 
    1010                 1015                 1020             


Asn Asp  Gln Trp Arg Asn Phe  Asn Phe Leu Asn Gly  Leu Arg Gln 
    1025                 1030                 1035             


Glu Gln  Gln Val Asp Ala Glu  Thr Gly Glu Leu Ile  Trp Gln Lys 
    1040                 1045                 1050             


Asp His  Asp Leu Ala Tyr Met  Asn Lys Ile Tyr Asp  Phe Lys Lys 
    1055                 1060                 1065             


Ile Leu  Val Thr Arg Glu Val  His Thr Asn His Gly  Ala Leu Tyr 
    1070                 1075                 1080             


Asn Gln  Thr Leu Tyr Lys Ala  Lys Asp Asp Lys Ala  Ser Gly Gln 
    1085                 1090                 1095             


Gly Gly  Arg Gln Leu Ile Ala  Ala Lys Glu Asn Arg  Pro Thr Ala 
    1100                 1105                 1110             


Ile Tyr  Gly Gly Tyr Ser Gly  Lys Thr Val Ala Tyr  Met Cys Leu 
    1115                 1120                 1125             


Val Arg  Ile Thr Glu Lys Lys  Gly Asp Phe Tyr Arg  Ile Cys Gly 
    1130                 1135                 1140             


Val Gln  Thr Ala Trp Val Asp  Glu Leu Glu His Ile  Glu Asp Ala 
    1145                 1150                 1155             


Thr Ile  Lys Leu Gln Arg Leu  Thr Ala Leu Leu Thr  Pro Gln Phe 
    1160                 1165                 1170             


Ser Ala  Thr Lys Lys Gln Lys  Asp Glu Val Val Thr  Val Val Lys 
    1175                 1180                 1185             


Pro Phe  Glu Val Leu Leu Pro  Lys Ile Leu Ile Asn  Gln Arg Phe 
    1190                 1195                 1200             


Tyr Asp  Gly Gly Gln Glu Leu  Thr Leu Ala Ser Ala  Thr Tyr Lys 
    1205                 1210                 1215             


Asn Asn  Ala Gln Glu Leu Val  Leu Asp Lys Lys Ala  Val Gln Val 
    1220                 1225                 1230             


Leu Asn  Gly Gln Leu Pro Lys  Ala Glu Leu Glu Ala  Gln Ala Thr 
    1235                 1240                 1245             


Val Val  Tyr Asp Gln Ile Leu  Glu Arg Val Gln Lys  Tyr Phe Pro 
    1250                 1255                 1260             


Leu Tyr  Asp Met Asn Ser Phe  Arg Glu Lys Leu Phe  Val Gly Arg 
    1265                 1270                 1275             


Asp Lys  Phe Asn Glu Leu Pro  Trp Gln Asp Gln Trp  Glu Asn His 
    1280                 1285                 1290             


Lys Leu  Ile Gln Val Gly Gln  Lys Thr Ile Leu Asp  Arg Ile Leu 
    1295                 1300                 1305             


Ile Gly  Leu His Ala Asn Ala  Ala Met Gly Asp Leu  Lys Ala Leu 
    1310                 1315                 1320             


Asn Met  Ser Thr Pro Phe Gly  Lys Leu Gln Lys Ser  Asn Gly Ile 
    1325                 1330                 1335             


Ser Leu  Ser Pro Glu Thr Gln  Ile Ile Tyr Gln Ser  Pro Thr Gly 
    1340                 1345                 1350             


Leu Phe  Glu Arg Arg Val Ala  Leu Lys Asp Leu 
    1355                 1360                 


<210>  972
<211>  1364
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  972

Met Lys Asn Leu Lys Glu Tyr Tyr Ile Gly Leu Asp Ile Gly Thr Ala 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Thr Asp Glu Ser Tyr Asn Ile Pro Lys Phe 
            20                  25                  30          


Asn Gly Lys Lys Met Trp Gly Val Arg Leu Phe Asp Asp Ala Lys Thr 
        35                  40                  45              


Ala Glu Glu Arg Arg Thr Gln Arg Gly Ser Arg Arg Arg Leu Asn Arg 
    50                  55                  60                  


Arg Lys Glu Arg Ile Asn Leu Leu Gln Asp Leu Phe Ala Thr Glu Ile 
65                  70                  75                  80  


Ser Lys Val Asp Pro Asn Phe Phe Leu Arg Leu Asp Asn Ser Asp Leu 
                85                  90                  95      


Tyr Arg Glu Asp Lys Asp Glu Lys Leu Lys Ser Lys Tyr Thr Leu Phe 
            100                 105                 110         


Asn Asp Lys Asp Phe Lys Asp Arg Asp Tyr His Lys Lys Tyr Pro Thr 
        115                 120                 125             


Ile His His Leu Ile Met Asp Leu Ile Glu Asp Glu Gly Lys Lys Asp 
    130                 135                 140                 


Ile Arg Leu Leu Tyr Leu Ala Cys His Tyr Leu Leu Lys Asn Arg Gly 
145                 150                 155                 160 


His Phe Ile Phe Glu Gly Gln Lys Phe Asp Thr Lys Asn Ser Phe Asp 
                165                 170                 175     


Lys Ser Ile Asn Asp Leu Lys Ile His Leu Arg Asp Glu Tyr Asn Ile 
            180                 185                 190         


Asp Leu Glu Phe Asn Asn Glu Asp Leu Ile Glu Ile Ile Thr Asp Thr 
        195                 200                 205             


Thr Leu Asn Lys Thr Asn Lys Lys Lys Glu Leu Lys Asn Ile Val Gly 
    210                 215                 220                 


Asp Thr Lys Phe Leu Lys Ala Ile Ser Ala Ile Met Ile Gly Ser Ser 
225                 230                 235                 240 


Gln Lys Leu Val Asp Leu Phe Glu Asp Gly Glu Phe Glu Glu Thr Thr 
                245                 250                 255     


Val Lys Ser Val Asp Phe Ser Thr Thr Ala Phe Asp Asp Lys Tyr Ser 
            260                 265                 270         


Glu Tyr Glu Glu Ala Leu Gly Asp Thr Ile Ser Leu Leu Asn Ile Leu 
        275                 280                 285             


Lys Ser Ile Tyr Asp Ser Ser Ile Leu Glu Asn Leu Leu Lys Asp Ala 
    290                 295                 300                 


Asp Lys Ser Lys Asp Gly Asn Lys Tyr Ile Ser Lys Ala Phe Val Lys 
305                 310                 315                 320 


Lys Phe Asn Lys His Gly Lys Asp Leu Lys Thr Leu Lys Arg Ile Ile 
                325                 330                 335     


Lys Lys Tyr Leu Pro Ser Glu Tyr Ala Asn Ile Phe Arg Asn Lys Ser 
            340                 345                 350         


Ile Asn Asp Asn Tyr Val Ala Tyr Thr Lys Ser Asn Ile Thr Ser Asn 
        355                 360                 365             


Lys Arg Thr Lys Ala Ser Lys Phe Thr Lys Gln Glu Asp Phe Tyr Lys 
    370                 375                 380                 


Phe Ile Lys Lys His Leu Asp Thr Ile Lys Glu Thr Lys Leu Asn Ser 
385                 390                 395                 400 


Ser Glu Asn Glu Asp Leu Lys Leu Ile Asp Glu Met Leu Thr Asp Ile 
                405                 410                 415     


Glu Phe Lys Thr Phe Ile Pro Lys Leu Lys Ser Ser Asp Asn Gly Val 
            420                 425                 430         


Ile Pro Tyr Gln Leu Lys Leu Met Glu Leu Lys Lys Ile Leu Asp Asn 
        435                 440                 445             


Gln Ser Lys Tyr Tyr Asp Phe Leu Asn Glu Ser Asp Glu Tyr Gly Thr 
    450                 455                 460                 


Val Lys Asp Lys Val Glu Ser Ile Met Glu Phe Arg Ile Pro Tyr Tyr 
465                 470                 475                 480 


Val Gly Pro Leu Asn Pro Asp Ser Lys Tyr Ala Trp Ile Lys Arg Glu 
                485                 490                 495     


Asn Thr Lys Ile Thr Pro Trp Asn Phe Lys Asp Ile Val Asp Leu Asp 
            500                 505                 510         


Ser Ser Arg Glu Glu Phe Ile Asp Arg Leu Ile Gly Arg Cys Thr Tyr 
        515                 520                 525             


Leu Lys Glu Glu Lys Val Leu Pro Lys Ala Ser Leu Ile Tyr Asn Glu 
    530                 535                 540                 


Phe Met Val Leu Asn Glu Leu Asn Asn Leu Lys Leu Asn Glu Phe Leu 
545                 550                 555                 560 


Ile Thr Glu Glu Met Lys Lys Ala Ile Phe Glu Glu Leu Phe Lys Thr 
                565                 570                 575     


Lys Lys Lys Val Thr Leu Lys Ala Val Ser Asn Leu Leu Lys Lys Glu 
            580                 585                 590         


Phe Asn Leu Thr Gly Asp Ile Leu Leu Ser Gly Thr Asp Gly Asp Phe 
        595                 600                 605             


Lys Gln Gly Leu Asn Ser Tyr Ile Asp Phe Lys Asn Ile Ile Gly Asp 
    610                 615                 620                 


Lys Val Asp Arg Asp Asp Tyr Arg Ile Lys Ile Glu Glu Ile Ile Lys 
625                 630                 635                 640 


Leu Ile Val Leu Tyr Glu Asp Asp Lys Thr Tyr Leu Lys Lys Lys Ile 
                645                 650                 655     


Lys Ser Ala Tyr Lys Asn Asp Phe Thr Asp Asp Glu Ile Lys Lys Ile 
            660                 665                 670         


Ala Ala Leu Asn Tyr Lys Asp Trp Gly Arg Leu Ser Lys Arg Phe Leu 
        675                 680                 685             


Thr Gly Ile Glu Gly Val Asp Lys Thr Thr Gly Glu Lys Gly Ser Ile 
    690                 695                 700                 


Ile Tyr Phe Met Arg Glu Tyr Asn Leu Asn Leu Met Glu Leu Met Ser 
705                 710                 715                 720 


Gly His Tyr Thr Phe Thr Glu Glu Val Glu Lys Leu Asn Pro Val Glu 
                725                 730                 735     


Asn Arg Glu Leu Cys Tyr Glu Met Val Asp Glu Leu Tyr Leu Ser Pro 
            740                 745                 750         


Ser Val Lys Arg Met Leu Trp Gln Ser Leu Arg Val Val Asp Glu Ile 
        755                 760                 765             


Lys Arg Ile Ile Gly Lys Asp Pro Lys Lys Ile Phe Ile Glu Met Ala 
    770                 775                 780                 


Arg Ala Lys Glu Ala Lys Asn Ser Arg Lys Glu Ser Arg Lys Asn Lys 
785                 790                 795                 800 


Leu Leu Glu Phe Tyr Lys Phe Gly Lys Lys Ala Phe Ile Asn Glu Ile 
                805                 810                 815     


Gly Glu Glu Arg Tyr Asn Tyr Leu Leu Asn Glu Ile Asn Ser Glu Glu 
            820                 825                 830         


Glu Ser Lys Phe Arg Trp Asp Asn Leu Tyr Leu Tyr Tyr Thr Gln Leu 
        835                 840                 845             


Gly Arg Cys Met Tyr Ser Leu Glu Pro Ile Asp Leu Ala Asp Leu Lys 
    850                 855                 860                 


Ser Asn Asn Ile Tyr Asp Gln Asp His Ile Tyr Pro Lys Ser Lys Ile 
865                 870                 875                 880 


Tyr Asp Asp Ser Leu Glu Asn Arg Val Leu Val Lys Lys Asn Leu Asn 
                885                 890                 895     


His Glu Lys Gly Asn Gln Tyr Pro Ile Pro Glu Lys Val Leu Asn Lys 
            900                 905                 910         


Asn Ala Tyr Gly Phe Trp Lys Ile Leu Phe Asp Lys Gly Leu Ile Gly 
        915                 920                 925             


Gln Lys Lys Tyr Thr Arg Leu Thr Arg Arg Thr Pro Phe Glu Glu Arg 
    930                 935                 940                 


Glu Leu Ala Glu Phe Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ala 
945                 950                 955                 960 


Thr Lys Glu Thr Ala Asn Leu Leu Lys Asn Ile Cys Gln Asp Ser Glu 
                965                 970                 975     


Ile Val Tyr Ser Lys Ala Glu Asn Ala Ser Arg Phe Arg Gln Glu Phe 
            980                 985                 990         


Asp Ile Ile Lys Cys Arg Thr Val  Asn Asp Leu His His  Met His Asp 
        995                 1000                 1005             


Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val Tyr Asn  Thr Lys Phe 
    1010                 1015                 1020             


Thr Lys  Asn Pro Leu Asn Phe  Ile Lys Asp Lys Asp  Asn Val Arg 
    1025                 1030                 1035             


Ser Tyr  Asn Leu Glu Asn Met  Phe Lys Tyr Asp Val  Val Arg Gly 
    1040                 1045                 1050             


Ser Tyr  Thr Ala Trp Ile Ala  Asp Asp Ser Glu Gly  Asn Val Lys 
    1055                 1060                 1065             


Ala Ala  Thr Ile Lys Lys Val  Lys Arg Glu Leu Glu  Gly Lys Asn 
    1070                 1075                 1080             


Tyr Arg  Phe Thr Arg Met Ser  Tyr Ile Gly Thr Gly  Gly Leu Tyr 
    1085                 1090                 1095             


Asp Gln  Asn Leu Met Arg Lys  Gly Lys Gly Gln Ile  Pro Gln Lys 
    1100                 1105                 1110             


Glu Asn  Thr Asn Lys Ser Asn  Ile Glu Lys Tyr Gly  Gly Tyr Asn 
    1115                 1120                 1125             


Lys Ala  Ser Ser Ala Tyr Phe  Ala Leu Ile Glu Ser  Asp Gly Lys 
    1130                 1135                 1140             


Ala Gly  Arg Glu Arg Thr Leu  Glu Thr Ile Pro Ile  Met Val Tyr 
    1145                 1150                 1155             


Asn Gln  Glu Lys Tyr Gly Asn  Thr Glu Ala Val Asp  Lys Tyr Leu 
    1160                 1165                 1170             


Lys Asp  Asn Leu Glu Leu Gln  Asp Pro Lys Ile Leu  Lys Asp Lys 
    1175                 1180                 1185             


Ile Lys  Ile Asn Ser Leu Ile  Lys Leu Asp Gly Phe  Leu Tyr Asn 
    1190                 1195                 1200             


Ile Lys  Gly Lys Thr Gly Asp  Ser Leu Ser Ile Ala  Gly Ser Val 
    1205                 1210                 1215             


Gln Leu  Ile Val Asn Lys Glu  Glu Gln Lys Leu Ile  Lys Lys Met 
    1220                 1225                 1230             


Asp Lys  Phe Leu Val Lys Lys  Lys Asp Asn Lys Asp  Ile Lys Val 
    1235                 1240                 1245             


Thr Ser  Phe Asp Asn Ile Lys  Glu Glu Glu Leu Ile  Lys Leu Tyr 
    1250                 1255                 1260             


Lys Thr  Leu Ser Asp Lys Leu  Asn Asn Gly Ile Tyr  Ser Asn Lys 
    1265                 1270                 1275             


Arg Asn  Asn Gln Ala Lys Asn  Ile Ser Glu Ala Leu  Asp Lys Phe 
    1280                 1285                 1290             


Lys Glu  Ile Ser Ile Glu Glu  Lys Ile Asp Val Leu  Asn Gln Ile 
    1295                 1300                 1305             


Ile Leu  Leu Phe Gln Ser Tyr  Asn Asn Gly Cys Asn  Leu Lys Ser 
    1310                 1315                 1320             


Ile Gly  Leu Ser Ala Lys Thr  Gly Val Val Phe Ile  Pro Lys Lys 
    1325                 1330                 1335             


Leu Asn  Tyr Lys Glu Cys Lys  Leu Ile Asn Gln Ser  Ile Thr Gly 
    1340                 1345                 1350             


Leu Phe  Glu Asn Glu Val Asp  Leu Leu Asn Leu 
    1355                 1360                 


<210>  973
<211>  1365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  973

Met Tyr Lys Lys Asp Tyr Thr Val Gly Leu Asp Ile Gly Ser Thr Ser 
1               5                   10                  15      


Val Gly Phe Ala Ala Ile Asp Asp Asp Tyr Gln Pro Ile Arg Leu Lys 
            20                  25                  30          


Gly Lys Arg Val Ile Gly Val Arg Leu Phe Glu Glu Gly Lys Thr Ala 
        35                  40                  45              


Ala Glu Arg Arg Asn Phe Arg Thr Thr Arg Arg Arg Leu Asn Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Ser Leu Leu Glu Glu Ile Phe Asp Pro Tyr Met Ala 
65                  70                  75                  80  


Lys Val Asp Pro Thr Phe Phe Ala Arg Leu Lys Gln Ser Asn Leu Phe 
                85                  90                  95      


Pro Lys Asp Lys Asn Lys Lys Phe Thr Gly Ser Leu Leu Phe Pro Lys 
            100                 105                 110         


Thr Thr Asp Ser Lys Phe Tyr Asp Glu Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Tyr His Leu Met Asn Asp Ser Arg Lys Phe Asp Leu Arg Glu Ile 
    130                 135                 140                 


Phe Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn 
145                 150                 155                 160 


Ser Ala Pro Ile Ala Ser Phe Ser Thr Asp Lys Ile Asn Leu Val Asp 
                165                 170                 175     


Asp Phe Glu Thr Val Asn Ala Ala Tyr Leu Gln Ile Asn Ser Ser Asn 
            180                 185                 190         


Pro Val Gln Leu Asp Thr Asp Gln Ala Asp Gln Val Ser Arg Ile Leu 
        195                 200                 205             


Leu Asp Asn Lys Leu Ser Asn Leu Asp Lys Gln Lys Gln Ala Ala Lys 
    210                 215                 220                 


Ala Leu Ala Gln Lys Ser Gly Asp Lys Lys Gln Asp Lys Ile Asn Asn 
225                 230                 235                 240 


Thr Thr Ile Lys Gln Ile Cys Lys Ala Leu Leu Gly Tyr Lys Phe Asp 
                245                 250                 255     


Leu Ala Val Val Leu Asn Ile Asp Ser Asp Asp His Asn Lys Trp Lys 
            260                 265                 270         


Leu Gln Leu Ser Asp Glu Asn Ile Asp Ser Arg Leu Ala Glu Leu Thr 
        275                 280                 285             


Gly Glu Leu Asn Glu Ala Gln Leu Asp Ile Leu Asn Val Leu Ser Arg 
    290                 295                 300                 


Leu Tyr Ser Gln Ile Thr Leu Asn Asp Ile Val Pro Ser Gly Met Thr 
305                 310                 315                 320 


Leu Ser Glu Ala Met Ile Glu Lys Tyr Lys Ala His Lys Lys His Leu 
                325                 330                 335     


Lys Met Leu Lys Asp Phe Ala Arg Ser Leu Ser Lys Lys Asp Tyr Gly 
            340                 345                 350         


Gln Val Met Glu Ala Tyr Ala Glu Tyr Ile Gly Asn Ile Pro Glu Ser 
        355                 360                 365             


Ala Ser Ser Lys Ala Gln Lys Lys Ile Ser Gln Asp Asp Phe Tyr Lys 
    370                 375                 380                 


Lys Ile Lys Arg Tyr Leu Asp Asp Ser Lys Leu Gly Gln Lys Ile Ala 
385                 390                 395                 400 


Glu Leu Ile Ala Lys Asn Glu Phe Met Pro Lys Gln Arg Thr Asn Gln 
                405                 410                 415     


Asn Gly Val Ile Pro Tyr Gln Leu His Leu Lys Glu Leu Asn Gln Ile 
            420                 425                 430         


Ile Glu Asn Gln Ser Lys Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro 
        435                 440                 445             


Asn Glu Lys Arg Arg Gly Lys Ala Lys Tyr Lys Leu Ser Glu Leu Val 
    450                 455                 460                 


Ala Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ile Thr Gln Glu Glu 
465                 470                 475                 480 


Gln Gln Lys Thr Ser Asn Ala Asn Phe Ala Trp Met Ile Arg Lys Ser 
                485                 490                 495     


Glu Gly Arg Ile Thr Pro Trp Asn Phe Asp Glu Lys Val Asp Arg Ile 
            500                 505                 510         


Ala Thr Ala Asn Arg Phe Ile Lys Arg Met Thr Thr Lys Asp Thr Tyr 
        515                 520                 525             


Leu Ile Gly Glu Asp Val Leu Pro Ala His Ser Leu Leu Tyr Glu Arg 
    530                 535                 540                 


Phe Lys Val Leu Asn Glu Leu Asn Met Ile Arg Val Asn Asn His Arg 
545                 550                 555                 560 


Leu Ser Val Gly Gln Lys Gln Asp Val Phe Glu Lys Leu Phe Gln Lys 
                565                 570                 575     


His Lys Thr Val Lys Thr Arg Glu Leu Ile Lys Gln Leu Met Ala Ser 
            580                 585                 590         


Gly Leu Pro Thr Gln Pro Arg Ile Thr Gly Leu Ser Asp Pro Ala Lys 
        595                 600                 605             


Phe Asn Thr Ser Leu Gly Thr Tyr Asn Asn Phe Ala Lys Ile Leu Gly 
    610                 615                 620                 


Asp Glu Ile Asn Gln Pro Asp Arg Gln Glu Asp Phe Glu Lys Ile Ile 
625                 630                 635                 640 


Glu Trp Ile Thr Ile Phe Asp Asp Lys Asp Ile Leu Lys Thr Lys Leu 
                645                 650                 655     


Ser Glu Leu Ser Trp Leu Asp Glu Lys Gln Ile Asn Ala Leu Ile Asn 
            660                 665                 670         


Lys Asn Tyr Ser Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Glu 
        675                 680                 685             


Leu Thr Asn Lys Lys Gly Gln Ser Ile Ile Asp Arg Met Trp Asn Thr 
    690                 695                 700                 


Gln Lys Ser Phe Ile Glu Ile Ile Asn Glu Pro Val Phe Ala Glu Gln 
705                 710                 715                 720 


Ile Lys Asp Ala Asn Gln Asp Gln Leu Asp Glu Asp Asp Tyr Glu Thr 
                725                 730                 735     


Val Leu His Asp Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Val Ile Lys Val Val Asp Asp Ile Val Arg Ala Ala Gly Arg Ala 
        755                 760                 765             


Pro Lys Phe Ile Ser Leu Glu Phe Ala Arg Glu Asp Gln Glu Ser Arg 
    770                 775                 780                 


Arg Thr Gln Ser Arg Leu Ser Arg Ile Lys Lys Ile Tyr Glu Thr Thr 
785                 790                 795                 800 


Thr Lys Glu Leu Val Ala Asn Asp Gln Ile Arg Glu Glu Leu Gly Asn 
                805                 810                 815     


Val Lys Asp Leu Asn Asp Arg Leu Phe Leu Tyr Phe Thr Gln Leu Gly 
            820                 825                 830         


Arg Asp Ala Tyr Thr Gly Glu Arg Ile Asn Ile Asp Asp Ile Ser Thr 
        835                 840                 845             


Lys Tyr Asp Ile Asp His Ile Phe Pro Arg Ala Phe Ile Lys Asp Asp 
    850                 855                 860                 


Ser Leu Asp Asn Lys Val Leu Val Lys Arg Ala Val Asn Asn Gly Lys 
865                 870                 875                 880 


Ser Asn Asn Val Pro Leu Lys Leu Phe Gly Ala Lys Met Lys Pro Ile 
                885                 890                 895     


Trp Lys Met Trp Lys Asp His Gln Leu Ile Ser Lys Arg Lys Tyr Glu 
            900                 905                 910         


His Leu Met Thr Asn Pro Asp Ala Ile Asp Lys Tyr Lys Ala Asn Gly 
        915                 920                 925             


Phe Ile Asn Arg Gln Leu Ile Glu Thr Arg Gln Ile Ile Lys Leu Ala 
    930                 935                 940                 


Ala Asn Ile Leu Ala Asn Arg Tyr Ser Thr Glu Asp Thr Lys Ile Ile 
945                 950                 955                 960 


Glu Val Lys Ala Asn Leu Asn His Gln Leu Arg Gln Ser Leu His Leu 
                965                 970                 975     


Tyr Lys Asn Arg Gln Val Asn Asp Tyr His His Ala Val Asp Gly Tyr 
            980                 985                 990         


Leu Ser Ala Phe Val Gly Gln Tyr  Leu Tyr His Arg Tyr  Pro Ser Leu 
        995                 1000                 1005             


Gln Ser  Tyr Phe Val Tyr Gly  Gln Tyr Gln Arg Phe  Phe Asp Lys 
    1010                 1015                 1020             


Gln Val  Gln Glu Asn Met Lys  Phe Asn Arg Phe Asn  Phe Leu Tyr 
    1025                 1030                 1035             


Asp Leu  Thr Asp Arg Asp Asn  Asp Gln Ile Val Val  Lys Ser Thr 
    1040                 1045                 1050             


Gly Glu  Ile Val Gly Glu Lys  Ser Lys Leu Ile Glu  Gln Ile Lys 
    1055                 1060                 1065             


Trp Val  Tyr Arg Tyr Lys Phe  Met Leu Val Ser Gln  Glu Val Phe 
    1070                 1075                 1080             


Thr Lys  Thr Gly Pro Leu Phe  Asp Gln Thr Ile Tyr  Pro Ala Gly 
    1085                 1090                 1095             


Ser Asn  Lys Ser Arg Leu Ile  Pro Ile Lys Lys Asp  Arg Pro Thr 
    1100                 1105                 1110             


Asp Ile  Tyr Gly Gly Tyr Ser  Ser Ser Thr Asp Ala  Tyr Met Ser 
    1115                 1120                 1125             


Leu Ile  Arg Val Pro Asp Lys  Lys Glu Asp Lys Tyr  Lys Val Val 
    1130                 1135                 1140             


Gly Val  Pro Ser Arg Ser Leu  Ala Ser Leu Asn Arg  Ala Glu Lys 
    1145                 1150                 1155             


Arg Gly  His Asp Ala Tyr Leu  Asp Gln Leu Arg Gln  Val Ile Ala 
    1160                 1165                 1170             


Lys Ser  Gln Lys Lys Glu Phe  Gln Ile Ile Ile Pro  Lys Leu Arg 
    1175                 1180                 1185             


Tyr Arg  Gln Leu Ile Ile Asp  Gly Gly Leu Lys Tyr  Thr Leu Gly 
    1190                 1195                 1200             


Ser Ser  Ala Tyr Gln Tyr Asn  Ala Arg Gln Leu Val  Leu Ser Glu 
    1205                 1210                 1215             


Lys Ser  Met Glu Val Leu Val  Thr Asp Phe Ser Lys  Val Thr Thr 
    1220                 1225                 1230             


Asp Leu  Asp Lys Gln Asn Gln  Lys Leu Ile Ala Val  Tyr Asp Asp 
    1235                 1240                 1245             


Ile Leu  Asp Lys Val Asn Arg  Cys Leu Pro Leu Tyr  Asp Lys Asn 
    1250                 1255                 1260             


Gln Phe  Arg Lys Ala Leu Asn  Gly Ala Arg Asp Lys  Phe Val Gln 
    1265                 1270                 1275             


Leu Pro  Ile Leu Pro Lys Phe  Asn Gly Asn Lys Lys  Glu Glu Ala 
    1280                 1285                 1290             


Gly Lys  Val Glu Ile Leu Asn  Glu Ile Leu Leu Gly  Leu His Asp 
    1295                 1300                 1305             


Asp Ala  Ser Thr Gly Asn Leu  Lys Pro Leu Gly Ile  Lys Thr Pro 
    1310                 1315                 1320             


Phe Gly  Met Leu Gln Thr Gln  Ser Gly Ile Thr Met  Ser Ser Asn 
    1325                 1330                 1335             


Ala Val  Ile Val Asn Gln Ser  Pro Thr Gly Leu Phe  Glu His Arg 
    1340                 1345                 1350             


Ile Tyr  Leu Lys Ala Leu Leu  Asn Lys Leu Asn Lys  
    1355                 1360                 1365 


<210>  974
<211>  1365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  974

Met Thr Lys Leu Asn Gln Pro Tyr Gly Ile Gly Leu Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Val Val Asp Ala Asn Ser His Leu Leu Arg 
            20                  25                  30          


Leu Lys Gly Glu Thr Ala Ile Gly Ala Arg Leu Phe Arg Glu Gly Gln 
        35                  40                  45              


Ser Ala Ala Asp Arg Arg Gly Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Thr Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Ala Pro His 
65                  70                  75                  80  


Ile Thr Lys Ile Asp Pro Asp Phe Phe Leu Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Asp Arg Phe Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Glu Asp Tyr Pro Ser Met Tyr 
        115                 120                 125             


His Leu Arg Leu His Leu Met Thr His Thr His Lys Ala Asp Pro Arg 
    130                 135                 140                 


Glu Ile Phe Leu Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Ala Ala Lys Asp Phe Asn Thr Asp Lys Val Asp Leu 
                165                 170                 175     


Glu Asp Ile Phe Pro Ala Leu Thr Glu Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Glu Leu Thr Phe Asp Leu Ala Lys Ala Asp Asp Phe Lys Ala 
        195                 200                 205             


Lys Leu Leu Asp Glu Gln Ala Thr Pro Ser Asp Thr Gln Lys Ala Leu 
    210                 215                 220                 


Val Lys Leu Leu Leu Ser Ser Asp Gly Glu Lys Glu Ile Val Lys Lys 
225                 230                 235                 240 


Arg Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Ile Thr Gly Leu Lys 
                245                 250                 255     


Thr Lys Phe Asn Leu Ala Leu Gly Thr Glu Val Asp Glu Ala Asp Ala 
            260                 265                 270         


Ser Asn Trp Gln Phe Ser Met Gly Gln Leu Asp Asp Lys Trp Ser Asn 
        275                 280                 285             


Ile Glu Thr Ser Met Thr Asp Gln Gly Thr Glu Ile Phe Glu Gln Ile 
    290                 295                 300                 


Gln Glu Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Met Ser Leu Ser Gln Ala Lys Val Ala Asp Tyr Gly Gln His Lys Glu 
                325                 330                 335     


Asp Leu Glu Leu Phe Lys Thr Tyr Leu Lys Lys Leu Asn Asp His Glu 
            340                 345                 350         


Leu Ala Lys Thr Ile Arg Gly Leu Tyr Asp Arg Tyr Ile Asn Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Leu Arg Glu Asp Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Asn Glu Val Ser Glu Gln Leu Leu Asn Arg 
385                 390                 395                 400 


Met Gly Gln Ala Asn Phe Met Leu Lys Gln Arg Thr Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Leu Gln Gln Arg Glu Leu Asp Gln Ile Ile Ala 
            420                 425                 430         


Asn Gln Ser Lys Tyr Tyr Asp Trp Leu Ala Lys Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Ala Ser Lys Pro Tyr Gln Leu Asp Glu Leu Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ile Thr Pro Lys Gln Gln Ala 
465                 470                 475                 480 


Glu Ser Gly Glu Asn Val Phe Ala Trp Met Val Arg Lys Asn Pro Ser 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Asp Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Lys Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Ile Asn Asn Glu Ala Leu 
545                 550                 555                 560 


Ser Thr Asp Gln Lys Gln Arg Leu Ile His Glu Val Phe Glu Arg His 
                565                 570                 575     


Ser Ser Val Thr Ile Lys Gln Val Val Asp Asn Leu Val Ala His Gly 
            580                 585                 590         


Asp Phe Ala Gln Arg Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr His Gln Leu Lys Glu Ile Leu His 
    610                 615                 620                 


Gly Ala Ile Asp Asp Ser Thr Lys Leu His Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Thr Trp Ser Thr Val Phe Glu Asp Ala Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Ala Glu Ile Asp Trp Leu Asp Pro Ala Thr Ile Lys Lys Leu Ser Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser Arg Lys Leu Leu Asp Gly 
        675                 680                 685             


Leu Lys Leu Gly Asn Gly His Thr Val Ile Gln Glu Leu Met Leu Ser 
    690                 695                 700                 


Asn His Asn Leu Met Gln Ile Leu Ala Asp Glu Thr Leu Lys Glu Thr 
705                 710                 715                 720 


Met Thr Glu Leu Asn Gln Asp Lys Leu Lys Thr Asp Asp Ile Glu Asp 
                725                 730                 735     


Val Ile Asn Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Arg Val Val Glu Asp Ile Lys His Ala Ala Asn Gly Gln 
        755                 760                 765             


Asp Pro Ser Trp Leu Tyr Ile Glu Thr Ala Asp Gly Thr Gly Thr Ala 
    770                 775                 780                 


Gly Lys Arg Thr Gln Ser Arg Gln Lys Gln Ile Gln Thr Val Tyr Ala 
785                 790                 795                 800 


Asn Ala Ala Gln Glu Leu Ile Asp Ser Ala Val Arg Gly Glu Leu Glu 
                805                 810                 815     


Asp Lys Ile Ala Asp Lys Ala Ser Phe Thr Asp Arg Leu Val Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Ala Pro Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser His Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu 
    850                 855                 860                 


Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Asn Ala Thr Ile 
865                 870                 875                 880 


Asn Arg Glu Lys Asn Asn Val Phe Ala Ser Thr Leu Phe Ala Ser Lys 
                885                 890                 895     


Met Ala Ala Thr Trp Arg Lys Trp His Glu Ala Gly Leu Ile Ser Gly 
            900                 905                 910         


Arg Lys Leu Arg Asn Leu Met Leu Arg Pro Asp Glu Thr Asp Lys Phe 
        915                 920                 925             


Ala His Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile 
    930                 935                 940                 


Lys Leu Thr Glu Gln Ile Ala Ala Ala Gln Tyr Pro Asn Thr Lys Ile 
945                 950                 955                 960 


Ile Ala Val Lys Ala Gly Leu Ser His Gln Leu Arg Glu Glu Leu Asp 
                965                 970                 975     


Phe Pro Lys Asn Arg Asp Val Asn His Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Phe Leu Ala Ala Arg Ile Gly Thr  Tyr Leu Leu Lys Arg  Tyr Pro Lys 
        995                 1000                 1005             


Leu Ala  Pro Phe Phe Thr Tyr  Gly Glu Phe Ala Lys  Val Asp Val 
    1010                 1015                 1020             


Lys Lys  Phe Arg Glu Phe Asn  Phe Ile Gly Ala Leu  Thr His Ala 
    1025                 1030                 1035             


Lys Lys  Asn Ile Ile Ala Lys  Asp Thr Gly Glu Ile  Val Trp Asp 
    1040                 1045                 1050             


Lys Glu  Arg Asp Ile Gln Glu  Leu Asp Arg Ile Tyr  Asn Phe Lys 
    1055                 1060                 1065             


Arg Met  Leu Ile Thr His Glu  Val His Phe Glu Thr  Ala Asp Leu 
    1070                 1075                 1080             


Phe Asp  Gln Thr Ile Tyr Ala  Ala Lys Asp Ser Lys  Glu Arg Gly 
    1085                 1090                 1095             


Gly Arg  Met Ser Leu Ile Pro  Lys Lys Leu Gly Tyr  Leu Thr Gly 
    1100                 1105                 1110             


Ile Tyr  Gly Gly Tyr Gln Thr  Glu Arg Gly Ser Tyr  Val Ser Ile 
    1115                 1120                 1125             


Ile Arg  Thr Phe Lys Lys Gly  Glu Thr Ile Tyr Lys  Val Val Asn 
    1130                 1135                 1140             


Ile Leu  Thr Ala Asp Val Ser  Leu Leu Ser Glu Lys  Glu Lys Val 
    1145                 1150                 1155             


Ser Lys  Thr Leu Ala Asp Ile  Tyr Leu Lys Glu Leu  Ile Ala Lys 
    1160                 1165                 1170             


Lys Leu  Ser Lys Gly Lys Ser  Gly Lys Asn Ile Ala  Ser Glu Pro 
    1175                 1180                 1185             


Phe Glu  Ile Val Leu Pro Arg  Val Gly Lys Asp Ser  Leu Phe Lys 
    1190                 1195                 1200             


Asn Lys  Lys Tyr Gly Leu Phe  Leu Ile Glu Ser Ala  Thr Glu Leu 
    1205                 1210                 1215             


His Asn  Tyr Gln Glu Leu Trp  Leu Pro Arg Asn Asp  Gln Leu Leu 
    1220                 1225                 1230             


Ile Lys  Arg Ile Gln Ala Arg  Gln Ile Glu Glu Ser  Lys Ala Asp 
    1235                 1240                 1245             


Tyr Glu  Leu Thr Arg Met Tyr  Arg Asn Ile Ala Trp  Gln Val Asp 
    1250                 1255                 1260             


His Tyr  Phe Arg Leu Tyr Lys  Ile Arg Lys Phe Ala  Glu Lys Leu 
    1265                 1270                 1275             


Gln Ala  Ser Val Glu Asp Phe  Ala Gly Leu Pro Val  Tyr Lys Asn 
    1280                 1285                 1290             


Glu Thr  Ser Asp Gly Lys Val  Glu Val Leu Ala Ser  Val Leu Lys 
    1295                 1300                 1305             


Arg Leu  Asn Gly Ala Ala Asp  Lys Gly Arg Thr Asn  Pro Thr Thr 
    1310                 1315                 1320             


Val Lys  Leu Gln Ser Asp Phe  Gly Arg Thr Leu Leu  Thr Asn Gly 
    1325                 1330                 1335             


Ile Val  Leu Glu Asp Asp Ser  Ser Ile Val Tyr Gln  Ser Pro Thr 
    1340                 1345                 1350             


Gly Leu  Phe Lys Arg Ser Val  Lys Leu Val Asp Leu  
    1355                 1360                 1365 


<210>  975
<211>  1365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  975

Met Thr Lys Glu Tyr Tyr Leu Gly Leu Asp Val Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Asp Ser Gln Tyr Asn Leu Cys Lys Phe Lys Lys 
            20                  25                  30          


Lys Asp Met Trp Gly Ile Arg Leu Phe Glu Ser Ala Asn Thr Ala Lys 
        35                  40                  45              


Asp Arg Arg Leu Gln Arg Gly Asn Arg Arg Arg Leu Glu Arg Lys Lys 
    50                  55                  60                  


Gln Arg Ile Asp Leu Leu Gln Glu Ile Phe Ser Pro Glu Ile Cys Lys 
65                  70                  75                  80  


Ile Asp Pro Thr Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Leu 
                85                  90                  95      


Glu Asp Lys Ser Asn Asp Phe Lys Tyr Pro Leu Phe Ile Glu Lys Asp 
            100                 105                 110         


Tyr Ser Asp Ile Glu Tyr Tyr Lys Glu Phe Pro Thr Ile Phe His Leu 
        115                 120                 125             


Arg Lys His Leu Ile Glu Ser Glu Glu Lys Gln Asp Ile Arg Leu Ile 
    130                 135                 140                 


Tyr Leu Ala Leu His Asn Ile Ile Lys Thr Arg Gly His Phe Leu Ile 
145                 150                 155                 160 


Asp Gly Asp Leu Gln Ser Ala Lys Gln Leu Arg Pro Ile Leu Asp Thr 
                165                 170                 175     


Phe Leu Leu Ser Leu Gln Glu Glu Gln Asn Leu Ser Val Ser Leu Ser 
            180                 185                 190         


Glu Asn Gln Lys Asp Glu Tyr Glu Glu Ile Leu Lys Asn Arg Ser Ile 
        195                 200                 205             


Ala Lys Ser Glu Lys Val Lys Lys Leu Lys Asn Leu Phe Glu Ile Ser 
    210                 215                 220                 


Asp Glu Leu Glu Lys Glu Glu Lys Lys Ala Gln Ser Ala Val Ile Glu 
225                 230                 235                 240 


Asn Phe Cys Lys Phe Ile Val Gly Asn Lys Gly Asp Val Cys Lys Phe 
                245                 250                 255     


Leu Arg Val Ser Lys Glu Glu Leu Glu Ile Asp Ser Phe Ser Phe Ser 
            260                 265                 270         


Glu Gly Lys Tyr Glu Asp Asp Ile Val Lys Asn Leu Glu Glu Lys Val 
        275                 280                 285             


Pro Glu Lys Val Tyr Leu Phe Glu Gln Met Lys Ala Met Tyr Asp Trp 
    290                 295                 300                 


Asn Ile Leu Val Asp Ile Leu Glu Thr Glu Glu Tyr Ile Ser Phe Ala 
305                 310                 315                 320 


Lys Val Lys Gln Tyr Glu Lys His Lys Thr Asn Leu Arg Leu Leu Arg 
                325                 330                 335     


Asp Ile Ile Leu Lys Tyr Cys Thr Lys Asp Glu Tyr Asn Arg Met Phe 
            340                 345                 350         


Asn Asp Glu Lys Glu Ala Gly Ser Tyr Thr Ala Tyr Val Gly Lys Leu 
        355                 360                 365             


Lys Lys Asn Asn Lys Lys Tyr Trp Ile Glu Lys Lys Arg Asn Pro Glu 
    370                 375                 380                 


Glu Phe Tyr Lys Ser Leu Gly Lys Leu Leu Asp Lys Ile Glu Pro Leu 
385                 390                 395                 400 


Lys Glu Asp Leu Glu Val Leu Thr Met Met Ile Glu Glu Cys Lys Asn 
                405                 410                 415     


His Thr Leu Leu Pro Ile Gln Lys Asn Lys Asp Asn Gly Val Ile Pro 
            420                 425                 430         


His Gln Val His Glu Val Glu Leu Lys Lys Ile Leu Glu Asn Ala Lys 
        435                 440                 445             


Lys Tyr Tyr Ser Phe Leu Thr Glu Thr Asp Lys Asp Gly Tyr Ser Val 
    450                 455                 460                 


Val Gln Lys Ile Glu Ser Ile Phe Arg Phe Arg Ile Pro Tyr Tyr Val 
465                 470                 475                 480 


Gly Pro Leu Ser Thr Arg His Gln Glu Lys Gly Ser Asn Val Trp Met 
                485                 490                 495     


Val Arg Lys Pro Gly Arg Glu Asp Arg Ile Tyr Pro Trp Asn Met Glu 
            500                 505                 510         


Glu Ile Ile Asp Phe Glu Lys Ser Asn Glu Asn Phe Ile Thr Arg Met 
        515                 520                 525             


Thr Asn Lys Cys Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys His 
    530                 535                 540                 


Ser Leu Leu Tyr Ser Lys Tyr Met Val Leu Asn Glu Leu Asn Asn Val 
545                 550                 555                 560 


Lys Val Arg Gly Lys Lys Leu Pro Thr Ser Leu Lys Gln Lys Val Phe 
                565                 570                 575     


Glu Asp Leu Phe Glu Asn Lys Ser Lys Val Thr Gly Lys Asn Leu Leu 
            580                 585                 590         


Glu Tyr Leu Gln Ile Gln Asp Lys Asp Ile Gln Ile Asp Asp Leu Ser 
        595                 600                 605             


Gly Phe Asp Lys Asp Phe Lys Thr Ser Leu Lys Ser Tyr Leu Asp Phe 
    610                 615                 620                 


Lys Lys Gln Ile Phe Gly Glu Glu Ile Glu Lys Glu Ser Ile Gln Asn 
625                 630                 635                 640 


Met Ile Glu Asp Ile Ile Lys Trp Ile Thr Ile Tyr Gly Asn Asp Lys 
                645                 650                 655     


Glu Met Leu Lys Arg Val Ile Arg Ala Asn Tyr Ser Asn Gln Leu Thr 
            660                 665                 670         


Glu Glu Gln Met Lys Lys Ile Thr Gly Phe Gln Tyr Ser Gly Trp Gly 
        675                 680                 685             


Asn Phe Ser Lys Met Phe Leu Lys Gly Ile Ser Gly Ser Asp Val Ser 
    690                 695                 700                 


Thr Gly Glu Thr Phe Asp Ile Ile Thr Ala Met Trp Glu Thr Asp Asn 
705                 710                 715                 720 


Asn Leu Met Gln Ile Leu Ser Lys Lys Phe Thr Phe Met Asp Asn Val 
                725                 730                 735     


Glu Asp Phe Asn Ser Gly Lys Val Gly Lys Ile Asp Lys Ile Thr Tyr 
            740                 745                 750         


Asp Ser Thr Val Lys Glu Met Phe Leu Ser Pro Glu Asn Lys Arg Ala 
        755                 760                 765             


Val Trp Gln Thr Ile Gln Val Ala Glu Glu Ile Lys Lys Val Met Gly 
    770                 775                 780                 


Cys Glu Pro Lys Lys Ile Phe Ile Glu Met Ala Arg Gly Gly Glu Lys 
785                 790                 795                 800 


Val Lys Lys Arg Thr Lys Ser Arg Lys Ala Gln Leu Leu Glu Leu Tyr 
                805                 810                 815     


Ala Ala Cys Glu Glu Asp Cys Arg Glu Leu Ile Lys Glu Ile Glu Asp 
            820                 825                 830         


Arg Asp Glu Arg Asp Phe Asn Ser Met Lys Leu Phe Leu Tyr Tyr Thr 
        835                 840                 845             


Gln Phe Gly Lys Cys Met Tyr Ser Gly Asp Asp Ile Asp Ile Asn Glu 
    850                 855                 860                 


Leu Ile Arg Gly Asn Ser Lys Trp Asp Arg Asp His Ile Tyr Pro Gln 
865                 870                 875                 880 


Ser Lys Ile Lys Asp Asp Ser Ile Asp Asn Leu Val Leu Val Asn Lys 
                885                 890                 895     


Thr Tyr Asn Ala Lys Lys Ser Asn Glu Leu Leu Ser Glu Asp Ile Gln 
            900                 905                 910         


Lys Lys Met His Ser Phe Trp Leu Ser Leu Leu Asn Lys Lys Leu Ile 
        915                 920                 925             


Thr Lys Ser Lys Tyr Asp Arg Leu Thr Arg Lys Gly Asp Phe Thr Asp 
    930                 935                 940                 


Glu Glu Leu Ser Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln 
945                 950                 955                 960 


Ser Thr Lys Ala Ile Ala Asp Ile Phe Lys Gln Ile Tyr Ser Ser Glu 
                965                 970                 975     


Val Val Tyr Val Lys Ser Ser Leu Val Ser Asp Phe Arg Lys Lys Pro 
            980                 985                 990         


Leu Asn Tyr Leu Lys Ser Arg Arg  Val Asn Asp Tyr His  His Ala Lys 
        995                 1000                 1005             


Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn Val Tyr  Asn Lys Lys 
    1010                 1015                 1020             


Phe Thr  Ser Asn Pro Ile Gln  Trp Met Lys Lys Asn  Arg Asp Thr 
    1025                 1030                 1035             


Asn Tyr  Ser Leu Asn Lys Val  Phe Glu His Asp Val  Val Ile Asn 
    1040                 1045                 1050             


Gly Glu  Val Ile Trp Glu Lys  Cys Thr Tyr His Glu  Asp Thr Asn 
    1055                 1060                 1065             


Thr Tyr  Asp Gly Gly Thr Leu  Asp Arg Ile Arg Lys  Ile Val Glu 
    1070                 1075                 1080             


Arg Asp  Asn Ile Leu Tyr Thr  Glu Tyr Ala Tyr Cys  Glu Lys Gly 
    1085                 1090                 1095             


Glu Leu  Phe Asn Ala Thr Ile  Gln Asn Lys Asn Gly  Asn Ser Thr 
    1100                 1105                 1110             


Val Ser  Leu Lys Lys Gly Leu  Asp Val Lys Lys Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Phe Ser  Ala Asn Thr Ser Tyr  Phe Ser Leu Ile Glu  Phe Glu Asp 
    1130                 1135                 1140             


Lys Lys  Gly Asp Arg Ala Arg  His Ile Ile Gly Val  Pro Ile Tyr 
    1145                 1150                 1155             


Ile Ala  Asn Met Leu Glu His  Ser Pro Ser Ala Phe  Leu Glu Tyr 
    1160                 1165                 1170             


Cys Glu  Gln Lys Gly Tyr Gln  Asn Val Arg Ile Leu  Val Glu Lys 
    1175                 1180                 1185             


Ile Lys  Lys Asn Ser Leu Leu  Ile Ile Asn Gly Tyr  Pro Leu Arg 
    1190                 1195                 1200             


Ile Arg  Gly Glu Asn Glu Val  Asp Thr Ser Phe Lys  Arg Ala Ile 
    1205                 1210                 1215             


Gln Leu  Lys Leu Asp Gln Lys  Asn Tyr Glu Leu Val  Arg Asn Ile 
    1220                 1225                 1230             


Glu Lys  Phe Leu Glu Lys Tyr  Val Glu Lys Lys Gly  Asn Tyr Pro 
    1235                 1240                 1245             


Ile Asp  Glu Asn Arg Asp His  Ile Thr His Glu Lys  Met Asn Gln 
    1250                 1255                 1260             


Leu Tyr  Glu Val Leu Leu Ser  Lys Met Lys Lys Phe  Asn Lys Lys 
    1265                 1270                 1275             


Gly Met  Ala Asp Pro Ser Asp  Arg Ile Glu Lys Ser  Lys Pro Lys 
    1280                 1285                 1290             


Phe Ile  Lys Leu Glu Asp Leu  Ile Asp Lys Ile Asn  Val Ile Asn 
    1295                 1300                 1305             


Lys Met  Leu Asn Leu Leu Arg  Cys Asp Asn Asp Thr  Lys Ala Asp 
    1310                 1315                 1320             


Leu Ser  Leu Ile Glu Leu Pro  Lys Asn Ala Gly Ser  Phe Val Val 
    1325                 1330                 1335             


Lys Lys  Asn Thr Ile Gly Lys  Ser Lys Ile Ile Leu  Val Asn Gln 
    1340                 1345                 1350             


Ser Val  Thr Gly Leu Tyr Glu  Asn Arg Arg Glu Leu  
    1355                 1360                 1365 


<210>  976
<211>  1365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  976

Met Asp Thr Ala Lys Thr Asn Lys Gln Pro Tyr Tyr Ile Gly Leu Asp 
1               5                   10                  15      


Met Gly Thr Ser Ser Val Gly Trp Ala Val Thr Asp Ala Ser Tyr Asn 
            20                  25                  30          


Val Val Lys Lys His Gly Lys Ala Leu Trp Gly Val Arg Leu Phe Glu 
        35                  40                  45              


Glu Ala Gln Thr Ala Ala Asp Arg Arg Leu His Arg Thr Ala Arg Arg 
    50                  55                  60                  


Arg Ile Gln Arg Arg Lys Gln Arg Leu Ala Leu Leu Gln Glu Leu Phe 
65                  70                  75                  80  


Ala Lys Glu Ile Cys Lys Val Asp Pro Gly Phe Phe Ile Arg Leu His 
                85                  90                  95      


Glu Ser Arg Leu Trp Gln Glu Asp Lys Ser Ile Asp Gln Pro Asn Thr 
            100                 105                 110         


Leu Phe Asn Asp Glu Asp Phe Asp Asp Lys Ala Tyr His Lys Ala Tyr 
        115                 120                 125             


Pro Thr Ile Tyr His Leu Arg Tyr Ala Leu Met Thr Glu Asn Lys Ser 
    130                 135                 140                 


Phe Asp Val Arg Leu Val Tyr Leu Ala Ile His His Ile Ile Lys His 
145                 150                 155                 160 


Arg Gly His Phe Leu Leu Glu Asn Tyr Asp Leu Ser Asn Gln Glu Thr 
                165                 170                 175     


Ser Gly Phe Asp Glu Ser Tyr Ile Ala Leu Thr Asn Ala Ile Lys Glu 
            180                 185                 190         


Leu Leu Gln Lys Glu Ile Pro Val Gly His Val Glu Lys Ile Gln Ile 
        195                 200                 205             


Ile Leu Lys Asp Lys Ser Leu Ser Lys Arg Glu Lys Thr Glu Lys Ile 
    210                 215                 220                 


Leu Gln Val Trp Asp Val Ile Lys Asp Lys Gln Leu Lys Glu Val Val 
225                 230                 235                 240 


Gln Leu Ile Cys Gly Gly Thr Ala Asn Leu Ser Thr Leu Phe Met Asp 
                245                 250                 255     


Glu Ser Leu Lys Glu Ala Leu Lys Val Phe Glu Ile Lys Asp Ser Lys 
            260                 265                 270         


Ile Ser Phe Ser Thr Ala Val Tyr Glu Glu Lys Glu Ser Asp Leu Gln 
        275                 280                 285             


Ser Val Leu Glu Gln Arg Phe Asp Leu Val Tyr Ala Ala Lys Met Leu 
    290                 295                 300                 


Tyr Asp Trp Ser Leu Leu Ala Glu Leu Met Gly Asp Arg Lys Thr Leu 
305                 310                 315                 320 


Ser Glu Ala Lys Ile Ala Ile Tyr Glu Thr His Lys Lys Asp Leu Glu 
                325                 330                 335     


Val Leu Lys Arg Leu Thr Arg Gln Asp Lys Ser Leu Tyr Arg Lys Leu 
            340                 345                 350         


Phe Arg Gly Thr Asp Lys Asp Ser Tyr Ser Ala Tyr Val Gly Ser Cys 
        355                 360                 365             


Met Ile His Gly Glu Lys Leu Val Ile Glu Lys Arg Gly Ser Thr Glu 
    370                 375                 380                 


Glu Phe Tyr Lys Phe Leu Lys Lys Glu Ile Ser Lys Leu Pro Asp Cys 
385                 390                 395                 400 


Glu Asp Lys Ile Tyr Val Leu Ser Lys Ile Glu Ala Glu Asn Phe Leu 
                405                 410                 415     


Pro Lys Ala Val Ser Thr Ile Asn Gly Ile Ile Pro Tyr Gln Leu Gln 
            420                 425                 430         


Glu Gln Glu Leu Asp Ile Ile Leu Lys Lys Ala Glu Gln Tyr Leu Pro 
        435                 440                 445             


Phe Leu Met Glu Lys Asp Ala Tyr Gly Thr Ile Ser Glu Lys Ile Lys 
    450                 455                 460                 


Gln Leu Leu Thr Phe Arg Ile Pro Phe Tyr Val Gly Pro Leu Asn Arg 
465                 470                 475                 480 


His Ser Asp Lys Ser Trp Ala Val His Thr Asp Lys Glu Gly Arg Ile 
                485                 490                 495     


Leu Pro Trp Asn Phe Asn Glu Arg Ile Asn Glu Glu Lys Ser Ala Glu 
            500                 505                 510         


Lys Phe Ile Thr Arg Met Thr Asn Lys Cys Thr Tyr Leu Leu Gly Lys 
        515                 520                 525             


Asp Val Leu Pro Lys Asn Ser Leu Leu Tyr Thr Glu Tyr Met Leu Phe 
    530                 535                 540                 


Asn Glu Leu Asn Asn Val Lys Ile Gly Lys Ala Gly Thr Tyr Leu Thr 
545                 550                 555                 560 


Glu Glu Gln Lys Glu Lys Leu Trp Lys Glu Leu Phe Leu Thr Thr Lys 
                565                 570                 575     


Lys Val Thr Leu Ser Lys Phe Ser Lys Phe Leu Ile Lys Glu Gly Ile 
            580                 585                 590         


Asp Lys Glu Glu Ala Val Glu Ile Lys Gly Leu Asp Gly Gly Phe Lys 
        595                 600                 605             


Ser Ser Leu Ala Pro Trp Ile Asp Leu Lys Asn Ile Leu Gly Glu Asp 
    610                 615                 620                 


Phe Thr Arg Asp Lys Ala Glu Glu Ile Ile Lys Ala Ile Thr Leu Phe 
625                 630                 635                 640 


Ser Met Asp Lys Lys Ile Leu Lys Lys Tyr Leu Met Arg Ile Val Ser 
                645                 650                 655     


Glu Pro Val Ala Lys Gln Leu Ser Lys Leu Arg Tyr Thr Gly Trp Gly 
            660                 665                 670         


Arg Phe Ser Lys Glu Phe Leu Thr Glu Ile Thr Pro Arg Val Thr Asp 
        675                 680                 685             


Asp Val Gln Val Leu Val Asp Thr Ser Thr Gly Glu Ile Met Asn Ile 
    690                 695                 700                 


Ile Thr Ala Leu Lys Lys Thr Ser Leu Asn Leu Met Glu Ile Leu Ser 
705                 710                 715                 720 


Ser Thr Tyr Gly Tyr Ser Ala Ala Ile Ser Glu Ala Asn Lys Glu Leu 
                725                 730                 735     


Val Gly Asn Thr Val Leu Ser Tyr Glu Thr Val Lys Glu Leu Thr Val 
            740                 745                 750         


Ser Pro Ala Val Lys Arg Pro Ile Trp Gln Thr Leu Lys Ile Ile Lys 
        755                 760                 765             


Glu Ile Thr His Ile Met Gly Ala Glu Pro Ala Arg Ile Phe Ile Glu 
    770                 775                 780                 


Met Ala Arg Asp Lys Gln Pro Asp Lys Gly Arg Thr Asp Ser Arg Lys 
785                 790                 795                 800 


Lys Gln Leu Ile Glu Leu Tyr Lys Lys Cys Lys Glu Asp Val Arg Ser 
                805                 810                 815     


Trp Ser Asn Arg Glu Ser Lys Asp Trp Val Glu Glu Ile Glu Ser Lys 
            820                 825                 830         


Ser Asp Ser Gln Leu Arg Ser Asn Lys Leu Phe Leu Tyr Tyr Thr Gln 
        835                 840                 845             


Met Gly Lys Cys Met Tyr Thr Gly Lys Pro Ile Asp Leu Gly Ala Leu 
    850                 855                 860                 


Leu Lys Gly Gln Leu Tyr Asp Arg Asp His Ile Tyr Pro Gln Ser Gln 
865                 870                 875                 880 


Thr Lys Asp Asp Ser Leu Asp Asn Leu Val Ile Val Asp Asn Arg Glu 
                885                 890                 895     


Asn Arg Asp Lys Ser Asn Thr Tyr Pro Leu Ser Lys Asn Ile Arg Glu 
            900                 905                 910         


Arg Gln Ile Gly Phe Trp Lys Leu Leu Arg Asp Lys Gly Phe Ile Ser 
        915                 920                 925             


Lys Glu Lys Tyr Phe Arg Leu Thr Arg Asn Thr Pro Phe Ser Asp Ala 
    930                 935                 940                 


Glu Lys Ala Glu Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser 
945                 950                 955                 960 


Thr Lys Ala Val Ala Glu Ile Leu Lys Arg Val Leu Pro Lys Ser Ile 
                965                 970                 975     


Ile Val Tyr Val Lys Ala Gly Leu Thr Ser Arg Phe Arg Gln Tyr Gly 
            980                 985                 990         


Glu Phe Ile Lys Val Arg Asp Leu  Asn Asp Tyr His His  Ala Lys Asp 
        995                 1000                 1005             


Ala Tyr  Leu Asn Ile Val Thr  Gly Asn Val His Tyr  Thr Lys Phe 
    1010                 1015                 1020             


Thr Ser  Asn Pro Ile Arg Phe  Val Lys Asp Cys Gln  Ile Tyr Ser 
    1025                 1030                 1035             


Leu Asn  Glu His Ala Leu Tyr  Lys Tyr Thr Val Glu  Arg Asn Gly 
    1040                 1045                 1050             


Val Val  Ala Trp Ala Pro Asn  Glu Glu Gly Met Met  Pro Ser Val 
    1055                 1060                 1065             


Thr Arg  Trp Met Asn Lys Asn  Asn Ile Leu Val Thr  Arg Met Ser 
    1070                 1075                 1080             


Tyr Val  Gly Gln Gly Glu Leu  Phe Asp Gln Leu Pro  Leu Lys Lys 
    1085                 1090                 1095             


Gly Lys  Gly Gln Val Ser Leu  Lys Lys Glu Gly Ala  Ile Ser Asp 
    1100                 1105                 1110             


Ile Ser  Lys Tyr Gly Gly Tyr  Asn Ser Ala Ser Thr  Ala Tyr Phe 
    1115                 1120                 1125             


Met Tyr  Val Glu Gly Glu Asp  Asn Lys Gly Lys Pro  Val Tyr Val 
    1130                 1135                 1140             


Ile Glu  Thr Met Pro Leu His  Leu Ala Ala Arg Leu  Thr Thr Leu 
    1145                 1150                 1155             


Glu Glu  Lys Gln Lys Tyr Cys  Glu Glu Leu Trp Met  Ser Thr Gly 
    1160                 1165                 1170             


Gly Lys  Leu Lys Asn Pro Lys  Val Leu Ile Glu Gln  Ile Pro Ile 
    1175                 1180                 1185             


Gln Ser  Leu Phe Ser Val Asp  Gly Phe Arg Val Thr  Ile Ala Gly 
    1190                 1195                 1200             


Lys Thr  Gly Lys Gln Tyr Gly  Cys Arg Leu Ala Glu  Glu Leu Val 
    1205                 1210                 1215             


Leu Asn  Asn Gly Tyr Ala Met  Thr Leu Lys Ala Val  Leu Lys Phe 
    1220                 1225                 1230             


Val Asn  Arg Arg Lys Met Asn  Lys Glu Ala Leu Ile  Thr Ser Tyr 
    1235                 1240                 1245             


Asp His  Leu Glu Glu Ser Gln  Leu Leu Ala Leu Tyr  Asp Val Phe 
    1250                 1255                 1260             


Leu Asp  Lys Leu Glu His Ser  Cys Phe Ser Lys Arg  Phe Ser Thr 
    1265                 1270                 1275             


Gln Arg  Asp Val Leu Lys Asn  Gly Arg Glu Ser Tyr  Ile Ala Leu 
    1280                 1285                 1290             


Ser Leu  Glu Asp Lys Cys Gln  Ile Leu Ser Glu Ile  Leu His Leu 
    1295                 1300                 1305             


Phe Gln  Cys Asn Ala Thr Leu  Ser Asn Leu Ser Leu  Leu Gly Ser 
    1310                 1315                 1320             


Ser Lys  Gln Ser Gly Arg Leu  Tyr Met Ser Lys Asn  Leu Ser Asp 
    1325                 1330                 1335             


Lys Asp  Ser Trp Tyr Ile Ile  His Gln Ser Val Thr  Gly Phe Tyr 
    1340                 1345                 1350             


Glu Gln  His Ile Pro Leu Ala  Pro Tyr Lys Lys Ser  
    1355                 1360                 1365 


<210>  977
<211>  1365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  977

Met Val Lys Lys Lys Tyr Ala Ile Gly Ile Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Val Val Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asn Thr Glu Lys Arg Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Thr Leu Leu Phe Asp Glu Gly Asn Thr Ala Glu Asn Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile 
65                  70                  75                  80  


Leu Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Lys Ile Asp Asp 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Ile Val Glu Asp Lys 
            100                 105                 110         


Gln Gly Ser Lys His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys Lys 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Ser Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Lys 
                165                 170                 175     


Ser Glu Asn Lys Asp Val Gln His Leu Phe Asn Asp Phe Val Glu Met 
            180                 185                 190         


Phe Asp Lys Thr Val Glu Gly Ser Tyr Leu Ser Glu Asn Leu Pro Asn 
        195                 200                 205             


Val Ala Asp Val Leu Val Glu Lys Val Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Ile Leu His Tyr Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly 
225                 230                 235                 240 


Asn Phe Leu Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Glu Leu Ala Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Ile Ala Thr Lys Ser Leu Tyr Asp Gly Ile Leu Leu Ala 
    290                 295                 300                 


Gly Ile Leu Ser Thr Thr Asp Ser Thr Thr Lys Ala Pro Leu Ser Ser 
305                 310                 315                 320 


Ser Met Val Asn Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Leu Leu 
                325                 330                 335     


Lys Asn Phe Ile His Gln Asn Leu Ser Asp Ser Tyr Lys Glu Val Phe 
            340                 345                 350         


Asn Asp Lys Leu Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Asn Phe Tyr Arg Phe Ile Lys Lys Ala Ile Glu Lys Ile 
    370                 375                 380                 


Glu Gly Ser Asp Tyr Phe Ile Asp Lys Ile Asp Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro 
            420                 425                 430         


Phe Leu Val Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Glu Phe Ala 
    450                 455                 460                 


Trp Leu Asn Arg Lys Ser Asp Glu Lys Ile Arg Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Glu Met Val Asp Lys Glu Thr Ser Ala Glu Asn Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Asn Tyr Asp Gln Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Met Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Asp Ile Val Lys Thr Leu Phe Lys Thr Lys Arg Lys Val 
545                 550                 555                 560 


Thr Ala Lys Asp Ile Lys Ala Tyr Leu Glu Asn Ser Asn Gly Tyr Ala 
                565                 570                 575     


Gly Val Glu Leu Lys Gly Leu Glu Glu Gln Phe Asn Ala Ser Leu Pro 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Leu Arg Asp Lys Ala Phe Ile Asp 
        595                 600                 605             


Ala Glu Glu Asn Gln Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Lys 
625                 630                 635                 640 


Asp Ile Leu Thr Glu Glu Gln Arg Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Leu Asp 
            660                 665                 670         


Lys Val Thr Arg Lys Thr Ile Leu Gly Tyr Leu Ile Asp Asp Gly Thr 
        675                 680                 685             


Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe 
    690                 695                 700                 


Val Asp Glu Ile Arg Leu Ala Gln Gly Ser Gly Glu Ala Glu Asp Tyr 
705                 710                 715                 720 


Arg Ala Glu Val Gln Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Ile Glu Val Met Gly 
            740                 745                 750         


Tyr Asp Pro Glu His Ile Val Val Glu Met Ala Arg Glu Asn Gln Phe 
        755                 760                 765             


Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Lys Ile Glu 
    770                 775                 780                 


Asn Ala Ile Lys Asn Leu Asp Ser Asn Leu Asn Ser Lys Ile Leu Lys 
785                 790                 795                 800 


Glu Tyr Pro Thr Asn Asn Gln Ala Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Glu Glu Leu Asp 
            820                 825                 830         


Ile Asp Gln Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Thr Lys Ser Ala 
    850                 855                 860                 


Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile Val His 
865                 870                 875                 880 


Lys Lys Lys Asn Phe Trp Lys Gln Leu Leu Asp Ser Gln Leu Ile Ser 
                885                 890                 895     


Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Asn Glu Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Thr Arg Phe Asn Thr 
    930                 935                 940                 


Lys Leu Asp Glu Ala Gly Asn Arg Ile Arg Asp Pro Lys Val Asn Ile 
945                 950                 955                 960 


Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Asp Tyr Gln 
                965                 970                 975     


Leu Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Thr  Ala Leu Leu Lys Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Ala  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Ser Arg Lys Ser Ala  Thr Glu Lys Val Leu  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Arg  Arg Val Leu Val Tyr  Ser Lys Thr 
    1040                 1045                 1050             


Gly Glu  Val Arg Ile Arg Pro  Val Ile Glu Val Asn  Lys Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Lys Ser Asp Phe Arg  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Tyr Pro Gln  Val Asn Val Val Lys  Lys Val Glu 
    1085                 1090                 1095             


Met Gln  Thr Gly Gly Phe Ser  Lys Glu Ser Ile Leu  Gln His Gly 
    1100                 1105                 1110             


Asp Ser  Asp Lys Leu Ile Pro  Arg Lys Thr Glu Lys  Phe Tyr Leu 
    1115                 1120                 1125             


Asp Thr  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Ile Ala Tyr 
    1130                 1135                 1140             


Ser Val  Leu Leu Ile Ala Asp  Ile Glu Lys Gly Lys  Ala Lys Lys 
    1145                 1150                 1155             


Leu Lys  Arg Val Lys Glu Leu  Ile Gly Ile Thr Ile  Met Glu Arg 
    1160                 1165                 1170             


Met Ala  Phe Glu Lys Asn Pro  Ile Glu Phe Leu Glu  His Lys Gly 
    1175                 1180                 1185             


Tyr Lys  Asn Ile Leu Glu Lys  Asn Ile Ile Lys Leu  Pro Lys Tyr 
    1190                 1195                 1200             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Arg Arg Leu  Leu Ala Ser 
    1205                 1210                 1215             


Ala Lys  Glu Leu Gln Lys Gly  Asn Glu Met Ile Leu  Pro Pro His 
    1220                 1225                 1230             


Leu Val  Thr Leu Leu Tyr His  Ser Ser Asn Ile His  Lys Ile Thr 
    1235                 1240                 1245             


Glu Pro  Ile His Leu Asn Tyr  Val Asn Lys Asn Lys  His Glu Phe 
    1250                 1255                 1260             


Lys Glu  Leu Leu Arg His Ile  Ser Asp Phe Ser Thr  Arg Tyr Ile 
    1265                 1270                 1275             


Leu Ala  Gln Asp Arg Leu Ser  Lys Ile Glu Glu Leu  Tyr Asp Lys 
    1280                 1285                 1290             


Asn Asp  Gly Asp Asp Ile Ser  Asp Leu Thr Ser Ser  Phe Val Asn 
    1295                 1300                 1305             


Leu Leu  Thr Phe Thr Ala Ile  Gly Ala Pro Ala Ala  Phe Lys Phe 
    1310                 1315                 1320             


Leu Gly  Ser Val Ile Asp Arg  Lys Arg Tyr Thr Ser  Ile Ala Glu 
    1325                 1330                 1335             


Ile Leu  Glu Ala Thr Leu Ile  His Gln Ser Val Thr  Gly Leu Tyr 
    1340                 1345                 1350             


Glu Thr  Arg Ile Asp Leu Ser  Lys Leu Gly Gly Asp  
    1355                 1360                 1365 


<210>  978
<211>  1366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  978

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asn Lys Lys Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ala Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Ala Glu Met Asn Lys Leu Asp Glu Ser 
                85                  90                  95      


Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Gly 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Gly Thr Leu Glu Glu Glu Lys Glu Tyr 
        115                 120                 125             


His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Ser Leu Ala Asp 
    130                 135                 140                 


Ser Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Asp Thr Phe Asp Ile 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Thr Ser Ile Tyr 
            180                 185                 190         


Asn Asn Ile Phe Glu Glu Ser Ser Leu Ser Asn Glu Asn Ala Gln Val 
        195                 200                 205             


Glu Glu Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 


Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Lys Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     


Asp Leu Glu Glu Met Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Ser Leu Leu Gly Gln Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             


Leu Phe Val Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ala Gly 
    290                 295                 300                 


Ile Leu Ser Val Lys Asp Pro Ala Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Glu Arg Tyr Asp Asn His Gln Asn Asp Leu Ser Ala Leu Lys 
                325                 330                 335     


Gln Phe Ile Arg Ser Asn Leu Pro Glu Lys Tyr Ala Glu Val Phe Ser 
            340                 345                 350         


Asp Asp Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             


Gln Glu Gly Phe Tyr Lys Tyr Ile Lys Asn Gln Ile Ser Lys Phe Glu 
    370                 375                 380                 


Gly Thr Lys Tyr Phe Leu Glu Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Asn Ala Ile Leu Gln Asn Gln Gly Gly Tyr Tyr Pro Phe 
            420                 425                 430         


Leu Lys Ala Asn Lys Glu Arg Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 


Leu Thr Arg Asn Ser Asp Asp Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     


Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Phe Ile Ala Glu Ala Met Lys Asp Tyr Glu Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 


Lys Arg Lys Ile Val Glu Asn Ile Phe Lys Asn His Arg Lys Val Thr 
545                 550                 555                 560 


Glu Lys Asp Ile Ile Asn Tyr Leu His Asn Val Asp Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp Asn 
        595                 600                 605             


Pro Lys Asn Gly Glu Ile Leu Glu Ser Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Leu Leu Ala Gln Tyr Asn Ser 
625                 630                 635                 640 


Val Phe Asp Glu Lys Val Ile Lys Ala Leu Ser Arg Lys His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         


Gln Thr Gly Asn Ser Ile Leu Asp Tyr Leu Met Asp Asp Gly Lys Ser 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Asp Gly Leu Ser Phe Lys 
    690                 695                 700                 


Glu Ile Ile Asp Lys Ala Gln Val Val Gly Glu Thr Asp Asn Leu Arg 
705                 710                 715                 720 


Gln Val Val Gln Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Ile Lys Leu Val Asp Glu Leu Val Lys Val Met Gly Ser 
            740                 745                 750         


Asp Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Asn Lys Gly Lys Ser Lys Ser Gln Gln Arg Leu Lys Thr Leu Ser Asp 
    770                 775                 780                 


Ala Ile Ser Glu Leu Gly Ser Asn Ile Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 


Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     


Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp Ile Asn Gln Leu Ser 
            820                 825                 830         


Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             


Ser Leu Asp Asn Ser Val Leu Thr Ser Ser Lys Asp Asn Arg Gly Lys 
    850                 855                 860                 


Ser Asp Asn Val Pro Ser Leu Glu Ile Val Glu Lys Met Lys Gly Phe 
865                 870                 875                 880 


Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys Phe Asn 
                885                 890                 895     


Asn Leu Thr Lys Val Glu Arg Gly Gly Leu Asp Glu Arg Asp Lys Val 
            900                 905                 910         


Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             


Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Ala Glu Val Asn Glu Lys 
    930                 935                 940                 


Asn Gln Lys Leu Arg Ser Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 


Val Ser Asn Phe Arg Lys Glu Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     


Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Ile Ala 
            980                 985                 990         


Lys Ala Ile Leu Lys Glu Tyr Pro  Lys Leu Glu Pro Glu  Phe Val Tyr 
        995                 1000                 1005             


Gly Asp  Tyr Gln Lys Tyr Asp  Leu Lys Arg Tyr Ile  Ser Arg Thr 
    1010                 1015                 1020             


Lys Asp  Pro Lys Glu Ile Glu  Lys Ala Thr Glu Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Asp Lys Val  Tyr Tyr Ala 
    1040                 1045                 1050             


Asp Gly  Thr Ile Ile Gln Arg  Gly Asn Val Glu Tyr  Ser Lys Asp 
    1055                 1060                 1065             


Thr Gly  Glu Ile Ala Trp Asn  Lys Lys Arg Asp Phe  Ala Ile Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Tyr Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Glu  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Gly Asn  Ser Asp Lys Leu Ile  Pro Arg Lys Thr Lys  Asn Ile Gln 
    1115                 1120                 1125             


Leu Asp  Thr Thr Lys Tyr Gly  Gly Phe Asp Ser Pro  Val Ile Ala 
    1130                 1135                 1140             


Tyr Ser  Ile Leu Leu Val Ala  Asp Val Glu Lys Gly  Lys Ser Lys 
    1145                 1150                 1155             


Lys Leu  Lys Thr Val Lys Ser  Leu Ile Gly Ile Thr  Ile Met Glu 
    1160                 1165                 1170             


Lys Val  Lys Phe Glu Ala Asn  Pro Val Ala Phe Leu  Glu Gly Lys 
    1175                 1180                 1185             


Gly Tyr  Gln Asn Val Val Glu  Glu Asn Ile Ile Arg  Leu Pro Lys 
    1190                 1195                 1200             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Arg Arg  Met Leu Ala 
    1205                 1210                 1215             


Ser Ser  Lys Glu Leu Gln Lys  Gly Asn Glu Met Val  Leu Pro Ser 
    1220                 1225                 1230             


Tyr Leu  Leu Ala Leu Leu Tyr  His Ala Lys Lys Ile  Gln Lys Lys 
    1235                 1240                 1245             


Asp Glu  Pro Glu His Leu Glu  Tyr Ile Lys Gln His  His Ser Glu 
    1250                 1255                 1260             


Phe Asn  Asp Leu Leu Asn Phe  Val Ser Asp Phe Ser  Gln Lys Tyr 
    1265                 1270                 1275             


Val Leu  Ala Glu Ser Asn Leu  Glu Lys Ile Lys Asn  Leu Tyr Ile 
    1280                 1285                 1290             


Asp Asn  Glu Gln Thr Asn Leu  Glu Glu Ile Ala Asn  Ser Phe Ile 
    1295                 1300                 1305             


Asn Leu  Leu Thr Phe Thr Ala  Phe Gly Ala Pro Ala  Val Phe Lys 
    1310                 1315                 1320             


Phe Phe  Gly Lys Asp Ile Glu  Arg Lys Arg Tyr Ser  Thr Val Thr 
    1325                 1330                 1335             


Glu Ile  Leu Lys Ala Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu 
    1340                 1345                 1350             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu Gly Glu  Glu 
    1355                 1360                 1365     


<210>  979
<211>  1366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  979

Met Asp Lys Lys Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Phe Val Ala Thr Asp Glu Asp Asn His Val Ile Arg Ala Lys Gly 
            20                  25                  30          


Lys Asn Val Ile Gly Val Arg Leu Phe Asp Glu Gly Gln Ser Ala Ala 
        35                  40                  45              


Glu Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Asn Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Asn Leu Leu Arg Glu Ile Phe Glu Pro His Ile Tyr Pro 
65                  70                  75                  80  


Ile Asp Ala Ser Phe Phe Met Arg Gln Lys Tyr Ser Asn Leu Ser Lys 
                85                  90                  95      


Gln Asp Pro Asn Gln Lys Ile Glu Phe Lys Asp Ile Leu Phe Ser Asp 
            100                 105                 110         


Lys Ser Asp Ala Glu Phe Tyr Arg Gln Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Gln Ala Leu Met Thr Glu Lys Arg Gln Phe Asp Leu Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Val Lys Tyr Arg Gly His Phe Leu Asp 
145                 150                 155                 160 


Glu Thr Pro Thr Asp Lys Phe Ser Phe Asp Gln Leu Asp Leu Thr Gln 
                165                 170                 175     


Glu Phe Glu Arg Leu Asn Val Leu Phe Ser Glu Gln Asn Pro Glu Glu 
            180                 185                 190         


Pro Phe Glu Ile Val Val Asn Gln Asn Gln Ser Ile Glu Asp Ile Leu 
        195                 200                 205             


Ser Asn Gln Ser Met Thr Ala Ser Asp Arg Arg Lys Gln Ala Leu Val 
    210                 215                 220                 


Val Phe Tyr Gln Gln Ser Glu Asn Lys Glu Asn Glu Lys Gln Tyr Lys 
225                 230                 235                 240 


Ala Ile Ala Thr Glu Ile Leu Asn Leu Leu Leu Gly Asn Lys Ala Lys 
                245                 250                 255     


Leu Asn Val Ile Leu Ala Gln Glu Val Asp Lys Glu Ala Ala Lys Asp 
            260                 265                 270         


Trp Ser Leu Ser Leu Ser Ser Glu Thr Phe Asp Asp Asp Leu Glu Lys 
        275                 280                 285             


Ala Ser Ser Gly Leu Asn Asp Ser Gln Leu Glu Met Val Gly Ile Phe 
    290                 295                 300                 


Glu Arg Ile Tyr Ser Ser Leu Ile Leu Lys Ser Ile Ile Gly Asp His 
305                 310                 315                 320 


Asn Ser Leu Ser Ala Ala Met Val Asp Lys Phe Lys Thr His Ala Gln 
                325                 330                 335     


His Arg Asp Leu Leu Lys Asp Val Ile Lys Asn Ser Asp Ala Thr Lys 
            340                 345                 350         


Gly Glu Gln Leu Gln Phe Ala Tyr Asp His Tyr Leu Gln Arg His Ser 
        355                 360                 365             


Ala Asp Gly Lys Ala Tyr Gly Lys Asp Asp Phe Tyr Lys Glu Val Lys 
    370                 375                 380                 


Lys Asn Leu Asp Asp Ser Asp Ala Ala Gln Gln Ile Gln Gln Leu Ile 
385                 390                 395                 400 


Asp Thr Asp Gln Phe Met Pro Lys Gln Arg Thr Lys Ala Asn Gly Ala 
                405                 410                 415     


Ile Pro His Gln Val Gln Gln Lys Glu Leu Asp Leu Ile Ile Glu Asn 
            420                 425                 430         


Gln Cys Gln Tyr Tyr Pro Trp Leu Ala Glu Glu Asn Pro Asn Ser Glu 
        435                 440                 445             


Arg His Asn Val Ala Lys Tyr Lys Leu Asp Glu Leu Val Ser Phe Arg 
    450                 455                 460                 


Val Pro Tyr Tyr Val Gly Pro Leu Ile Ser Pro Ala Asp Asp Glu Thr 
465                 470                 475                 480 


Asp Pro Gln Ala Ile Gln Asn Thr Lys Phe Ala Trp Met Ile Arg Lys 
                485                 490                 495     


Asp Ala Gln Gly Arg Ile Thr Pro Trp Asn Phe Asp Asp Lys Val Asp 
            500                 505                 510         


Arg Glu Lys Ser Ala Asp Lys Phe Ile Lys Arg Met Thr Thr Thr Asp 
        515                 520                 525             


Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Asp Gln Ser Leu Ile Tyr 
    530                 535                 540                 


Gln Arg Tyr Lys Val Leu Asn Glu Leu Asn Asn Val Arg Ile Asn Gly 
545                 550                 555                 560 


Arg Pro Ile Glu Val Gln Ile Lys Gln Asn Ile Tyr Thr Asp Leu Phe 
                565                 570                 575     


Lys Lys Phe Lys Ser Val Ser Val Lys Lys Leu Arg Ser Tyr Leu Glu 
            580                 585                 590         


Glu Met Ser Tyr Tyr Pro Gly Ser Phe Lys Ile Ser Gly Leu Thr Asn 
        595                 600                 605             


Pro Gly Lys Phe Asp Asn Gly Leu Thr Ser Tyr Leu Asp Tyr Lys Asn 
    610                 615                 620                 


Ile Leu Gly Asp Ser Val Asp Ala Asn Asp Lys Arg Ala Asp Ile Glu 
625                 630                 635                 640 


Gln Met Ile Glu Trp Ser Thr Ile Phe Glu Asp Asn Lys Ile Phe Ala 
                645                 650                 655     


Gln Lys Leu Glu Ser Met Thr Trp Leu Thr Ala Glu Gln Arg Lys Ala 
            660                 665                 670         


Ile Ser Arg Lys Arg Tyr Thr Gly Trp Gly Arg Leu Ser Asn Lys Leu 
        675                 680                 685             


Leu Thr Gln Leu Lys Asn Gln Asp Asn Arg Ser Ile Met Asp Leu Leu 
    690                 695                 700                 


Trp Glu Thr Glu Glu Asn Phe Met Lys Ile Gln Ser Ala Pro Asp Phe 
705                 710                 715                 720 


Ser Lys Met Ile Glu Ala His Asn Gln Gln Phe Ile Lys Asn Glu Ala 
                725                 730                 735     


Asn Ile Gln Gln Val Ile Ser Asp Leu Tyr Thr Ser Pro Gln Asn Lys 
            740                 745                 750         


Lys Ala Ile Arg Gln Ile Leu Leu Val Val Asp Asp Ile Gln Lys Ala 
        755                 760                 765             


Val Gly His Ala Pro Glu His Ile Ala Ile Glu Phe Ala Lys Gly Glu 
    770                 775                 780                 


Asp Lys Asn Pro Arg Arg Thr Ile Ser Arg Glu Met Lys Ile Gln Asp 
785                 790                 795                 800 


Val Tyr Gln Gln Ile Ser Asp Asp Ile Leu Val Ser Pro Glu Val Arg 
                805                 810                 815     


Ala Gln Leu Asn Glu Lys Ile Glu Asn Arg His Gly Arg Leu Ser Asp 
            820                 825                 830         


Arg Leu Phe Leu Tyr Phe Met Gln Gly Gly Arg Asp Met Tyr Thr Gly 
        835                 840                 845             


Lys Pro Ile Asn Ile Asn Asn Ile Ser His Tyr Asp Ile Asp His Ile 
    850                 855                 860                 


Leu Pro Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu 
865                 870                 875                 880 


Val Ser Asp Lys Leu Asn Arg Glu Lys Ser Asp Thr Val Pro Arg Asp 
                885                 890                 895     


Leu Phe Gly Arg Glu Met Met Ser Thr Trp Glu Lys Met Ala Asp Arg 
            900                 905                 910         


Gly Leu Ile Thr Lys Arg Lys Leu Ala Asn Leu Lys Thr Ile Pro Gly 
        915                 920                 925             


Ser Ile Asp Ala His Lys Ala Gln Gly Phe Ile Asn Arg Gln Leu Val 
    930                 935                 940                 


Glu Thr Arg Gln Val Ile Lys Leu Ala Val Gly Ile Met Ile Asp Gln 
945                 950                 955                 960 


Tyr Pro Glu Thr Gln Ile Val Ser Val Lys Ala Asn Leu Thr His Glu 
                965                 970                 975     


Met Arg Glu Met Phe Asp Leu Val Lys Asn Arg Asp Val Asn Asp Tyr 
            980                 985                 990         


His His Ala Phe Asp Ala Tyr Leu  Thr Ala Phe Val Gly  Asn Tyr Leu 
        995                 1000                 1005             


Leu Gln  Arg Tyr Pro Lys Leu  Glu Pro Tyr Phe Val  Tyr Asp Lys 
    1010                 1015                 1020             


Phe Lys  Lys Leu Lys Asp Gln  Asn Ile Ser Met Arg  Asp Phe Asn 
    1025                 1030                 1035             


Leu Leu  Tyr Asp Leu Lys Lys  Asn Glu Asp Val Tyr  Asp Gln Arg 
    1040                 1045                 1050             


Thr Gly  His Ser Ala His Lys  Ile Trp Asp Gln His  Gly Ser Ile 
    1055                 1060                 1065             


Asp Tyr  Ile Arg His Leu Phe  Ala Tyr Lys Lys Ile  Leu Val Ser 
    1070                 1075                 1080             


His Glu  Val His Gln Asn Gly  Gly Ala Leu Tyr Asn  Met Thr Val 
    1085                 1090                 1095             


Tyr Gly  Lys Ala Asp Asp Ala  Lys Arg Lys Leu Ile  Pro Ile Lys 
    1100                 1105                 1110             


Ala Asp  Lys Pro Thr Glu Leu  Tyr Gly Gly Tyr Thr  Ser Arg Lys 
    1115                 1120                 1125             


Leu Ala  Tyr Met Ala Ile Val  Lys Thr Lys Lys Gly  Lys Lys Glu 
    1130                 1135                 1140             


Gln Tyr  Gln Val Thr Gly Val  Pro Val Ser Ala Leu  Thr Thr Leu 
    1145                 1150                 1155             


Arg Gln  Phe Lys Glu Asp Ser  Pro Glu Gln Lys Gln  Ala Leu Glu 
    1160                 1165                 1170             


Glu Ile  Val Arg Arg Asn Phe  Asp Glu Lys Thr Glu  Val Glu Ile 
    1175                 1180                 1185             


Ile Val  Pro Lys Val Leu Phe  Asn Gln Leu Ile Val  Asp Glu Lys 
    1190                 1195                 1200             


Cys Arg  Phe Met Val Gly Ser  Asp Thr Tyr Arg Tyr  Asn Ala Gln 
    1205                 1210                 1215             


Gln Leu  Val Leu Ser Ala Asp  Asp Leu Glu Ile Ile  Asn Ala Ser 
    1220                 1225                 1230             


His Lys  Asp Ile Gln Thr Ile  Asp Ser Ser Asp Tyr  Ser Gln Gln 
    1235                 1240                 1245             


Leu Asp  Gln Val Tyr Gln Asn  Ile Leu Thr Ile Val  Asp Lys His 
    1250                 1255                 1260             


Phe Pro  Met Tyr Asp Ile Asn  Lys Phe Arg Thr Lys  Leu His Asp 
    1265                 1270                 1275             


Ile Tyr  Glu Gln Tyr Ile Glu  Leu Pro Asn Glu Asp  Val Trp Glu 
    1280                 1285                 1290             


Asn Asn  Lys Leu Thr Thr Leu  Gly Lys His Thr Ile  Ile Ile Arg 
    1295                 1300                 1305             


Ile Leu  Gln Gly Leu His Ala  Asn Ala Thr Val Val  Asp Leu Lys 
    1310                 1315                 1320             


Gln Leu  Gly Ile Lys Thr Pro  Phe Gly Phe Met Gln  Gln Ala Arg 
    1325                 1330                 1335             


Gly Ile  Val Val Ser Pro Asp  Ala Gln Leu Ile Tyr  Gln Ser Pro 
    1340                 1345                 1350             


Thr Gly  Leu Tyr Glu Arg Arg  Val Arg Leu Gly Asp  Leu 
    1355                 1360                 1365     


<210>  980
<211>  1366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  980

Met Gly Asp Arg Lys Tyr Asn Leu Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Phe Ala Ala Val Asp Glu Asn Asn Gln Pro Ile Arg Val Lys 
            20                  25                  30          


Gly Lys Thr Ala Ile Gly Val Arg Leu Phe Glu Glu Gly Lys Thr Ala 
        35                  40                  45              


Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Arg Trp Arg Ile Asn Leu Leu Asn Gly Ile Phe Asp Ala His Leu Ala 
65                  70                  75                  80  


Glu Val Asp Pro Thr Phe Leu Ala Arg Leu Lys Glu Ser Asn Arg Ser 
                85                  90                  95      


Asn Leu Asp Pro Lys Lys Ser Phe Gln Gly Ser Leu Leu Phe Pro Glu 
            100                 105                 110         


Arg Lys Asp Tyr Gln Phe Tyr Lys Glu Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Lys Ala Leu Met Glu Glu Asp Arg Lys Phe Asp Ile Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Val His His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Asn 
145                 150                 155                 160 


Gly Thr Pro Met Arg Ser Phe Lys Val Glu Asn Ile Glu Leu Asp Thr 
                165                 170                 175     


Leu Phe Asp Arg Leu Asn Gln Leu Tyr Glu Glu Ile Ile Pro Asp Asn 
            180                 185                 190         


Glu Leu Ala Phe Asp Leu Glu Gln Val Ala Asp Val Lys Glu Val Leu 
        195                 200                 205             


Ser Ser Thr Thr Ile Tyr Lys Met Asp Lys Lys Lys Gln Leu Val Lys 
    210                 215                 220                 


Met Met Leu Leu Pro Thr Ser Asn Lys Ala Leu Gln Ser Glu Asn Lys 
225                 230                 235                 240 


Lys Ile Val Thr Gln Phe Val Asn Ala Ile Leu Asn Tyr Lys Phe Lys 
                245                 250                 255     


Leu Asp Val Leu Leu Gln Val Glu Thr Asp Ala Asp Trp Ser Leu Lys 
            260                 265                 270         


Leu Asn Asp Glu Gly Ala Asp Asp Lys Leu Glu Glu Phe Thr Gly Asp 
        275                 280                 285             


Leu Asp Glu Asn Arg Leu Glu Ile Ile Asp Leu Leu Gln Arg Leu His 
    290                 295                 300                 


Asn Trp Phe Ser Leu Asn Glu Ile Thr Lys Asp Gly Asn Ser Leu Ser 
305                 310                 315                 320 


Ala Ala Met Val Glu Lys Tyr Lys Asp His Arg His His Leu Glu Leu 
                325                 330                 335     


Leu Lys Lys Val Ile Glu Asp His Pro Asp Ala Lys Lys Ala Lys Ala 
            340                 345                 350         


Leu Lys Glu Thr Tyr Thr Ala Tyr Val Gly Lys Thr Asp Asp Lys Thr 
        355                 360                 365             


Gln Asn Gln Asp Asp Phe Tyr Lys Ala Val Glu Lys Asn Leu Asp Asp 
    370                 375                 380                 


Ser Pro Asp Ala Lys Glu Ile Lys Arg Leu Ile Gln Leu Asp Gln Phe 
385                 390                 395                 400 


Met Pro Lys Gln Arg Thr Gly Gln Asn Gly Ala Ile Pro His Gln Leu 
                405                 410                 415     


His Gln Gln Glu Leu Asp Gln Ile Ile Glu Lys Gln Ser Lys Tyr Tyr 
            420                 425                 430         


Pro Phe Leu Ala Glu Pro Asn Pro Asn Val Lys Arg Arg Lys Asp Ala 
        435                 440                 445             


Pro Tyr Lys Leu Asp Glu Leu Ile Ala Phe Lys Ile Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Leu Val Thr Pro Glu Glu Gln Ala Gln Asn Gly Glu Asn Val 
465                 470                 475                 480 


Phe Ala Trp Met Lys Arg Lys Val Ala Gly Pro Ile Thr Pro Trp Asn 
                485                 490                 495     


Phe Asp Lys Lys Val Asp Arg Met Glu Ser Ala Asn Arg Phe Ile Arg 
            500                 505                 510         


Arg Met Thr Thr Lys Asp Thr Tyr Leu Phe Gly Glu Asp Val Leu Pro 
        515                 520                 525             


Ala Glu Ser Met Ile Tyr Glu Lys Phe Val Val Leu Asn Glu Leu Asn 
    530                 535                 540                 


Asn Leu Lys Ile Asn Gly Arg His Leu Ser Leu Lys Asp Lys Gln Asp 
545                 550                 555                 560 


Ile Tyr Asn Asp Leu Phe Lys Gln Gln Lys Thr Val Ser Ile Lys Ala 
                565                 570                 575     


Leu Gln Asn Tyr Tyr Val Thr Lys Lys Lys Ala Ala Thr Ala Pro Thr 
            580                 585                 590         


Val Gly Gly Leu Ala Asp Pro Lys Lys Phe Leu Ser Ser Leu Ser Thr 
        595                 600                 605             


Tyr Ile Asp Phe Lys Asn Met Phe Gly Glu Gln Val Asn Asp Pro Gln 
    610                 615                 620                 


Phe Gln Glu Asp Leu Glu Gln Ile Val Glu Trp Ser Thr Ile Phe Glu 
625                 630                 635                 640 


Asp Arg Gly Ile Phe Lys Ala Lys Leu Gln Ala Leu Gly Trp Leu Ser 
                645                 650                 655     


Glu Lys Gln Ile Gln Gln Leu Val Ala Lys Arg Tyr Lys Gly Trp Gly 
            660                 665                 670         


Arg Leu Ser Lys Lys Leu Leu Thr Gly Leu Lys Asn Ala Glu Gly Tyr 
        675                 680                 685             


Ser Ile Leu Asp Glu Met Trp Arg Ser Thr Glu Asn Phe Met Gln Ile 
    690                 695                 700                 


Gln Ser Arg Pro Glu Phe Ala Ala Leu Ile Gln Gln Ala Asn Glu Lys 
705                 710                 715                 720 


Gln Phe Glu Gly Asn Asp Pro Asn Asn Val Trp Glu Asn Ile Glu Asn 
                725                 730                 735     


Ile Leu Gly Asp Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Val Val Lys Val Val Gln Asp Ile Glu Lys Ala Val Gly Asn Pro 
        755                 760                 765             


Pro Glu Lys Ile Ala Ile Glu Phe Thr Arg Glu Ala Ala Ala Asn Pro 
    770                 775                 780                 


Gln Arg Thr Gln Ser Arg Leu Arg Thr Leu Glu Lys Leu Tyr Glu Ser 
785                 790                 795                 800 


Ala Glu Glu Val Val Asp Ala Arg Leu Thr Ala Glu Leu Ala Glu Phe 
                805                 810                 815     


Lys Glu Asn Lys His Val Leu Ser Asp Lys Tyr Tyr Leu Tyr Phe Thr 
            820                 825                 830         


Gln Leu Gly Arg Asp Val Tyr Thr Gly Asp Thr Ile Ser Leu Asp Arg 
        835                 840                 845             


Leu Asn Asp Tyr Asp Val Asp His Ile Leu Pro Gln Ser Phe Ile Lys 
    850                 855                 860                 


Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ile Arg Ala Val Asn Asn 
865                 870                 875                 880 


Gly Lys Ser Asp Asn Val Pro Ala Lys Met Phe Gly Lys Lys Met Gly 
                885                 890                 895     


Ser Phe Trp Arg Tyr Leu Leu Asp Asn Gly Met Ile Ser Lys Arg Lys 
            900                 905                 910         


Tyr Asn Asn Leu Ile Thr Asp Pro Asp Asn Ile Ser Lys Tyr Ala Gln 
        915                 920                 925             


Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys 
    930                 935                 940                 


Leu Thr Ala Asn Ile Leu Asn Gly Ile Tyr Asp Lys Asp Thr Glu Ile 
945                 950                 955                 960 


Ile Glu Val Pro Ala Lys Met Asn Ser Gln Met Arg Lys Met Phe Asp 
                965                 970                 975     


Leu Val Lys Val Arg Glu Val Asn Asp Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Tyr Leu Thr Ile Phe Ile Gly Asn  Tyr Leu Tyr Lys Cys  Tyr Pro Lys 
        995                 1000                 1005             


Leu Arg  Pro Tyr Phe Val Tyr  Gly Lys Phe Lys Lys  Phe Ser Asp 
    1010                 1015                 1020             


Asn Glu  Asp Ile Glu Val Gly  Asp Gly Arg Phe Asn  Phe Leu Asn 
    1025                 1030                 1035             


Arg Ile  Glu Arg Leu Lys Asn  Val Thr Asp Pro Glu  Thr Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Ser Asn Val Ala  Pro Asn Glu Thr Ile  Lys Arg Ile 
    1055                 1060                 1065             


Lys Lys  Val Tyr Asn Tyr Lys  Phe Met Ile Val Ser  Arg Glu Ile 
    1070                 1075                 1080             


Thr Thr  Arg Arg Ala Glu Leu  Phe Asn Gln Thr Val  Tyr Pro Lys 
    1085                 1090                 1095             


Asn Tyr  Arg Gly Lys Leu Ile  Pro Ile Lys Glu Asp  Arg Pro Thr 
    1100                 1105                 1110             


Asp Leu  Tyr Gly Gly Tyr Ser  Gly Asn Thr Asp Ala  Tyr Leu Ala 
    1115                 1120                 1125             


Ile Val  Ala Leu Glu Asp Lys  Lys Lys Gly Lys Tyr  Phe Lys Val 
    1130                 1135                 1140             


Val Gly  Ile Pro Thr Arg Ala  Ala Ala Lys Leu Glu  Lys Leu Lys 
    1145                 1150                 1155             


Gln Gln  Asp Ser Gln Gln Tyr  Leu Gln Ala Val His  Glu Val Ile 
    1160                 1165                 1170             


Ala Pro  Gln Phe Thr Lys Ser  Thr Lys Lys Gly Ile  Lys Lys Thr 
    1175                 1180                 1185             


Glu Phe  Arg Ile Val Leu Asp  Lys Val His Tyr Arg  Gln Leu Val 
    1190                 1195                 1200             


Gln Asp  Gly Pro Val Lys Met  Met Leu Gly Ser Ser  Thr Tyr Lys 
    1205                 1210                 1215             


Tyr Asn  Ala Lys Gln Leu Val  Leu Ser Glu Lys Ala  Leu Gln Val 
    1220                 1225                 1230             


Ile Lys  Asp Asp Arg Lys Phe  Asp Glu Thr Gln Lys  Asp Asp Asn 
    1235                 1240                 1245             


Leu Ile  Ala Val Tyr Asp Glu  Ile Leu Ser Ile Val  Asn Gln Ser 
    1250                 1255                 1260             


Phe Asp  Leu Tyr Asp Ile Asn  Gly Phe Arg Lys Lys  Leu Asn Asp 
    1265                 1270                 1275             


Asn Arg  Asn Gln Phe Ile Asp  Leu Pro Ala Glu Thr  Lys Tyr Glu 
    1280                 1285                 1290             


Gly Arg  Lys Val Val Ala His  Gly Lys Arg Glu Met  Ile Leu Glu 
    1295                 1300                 1305             


Ile Leu  Lys Gly Leu His Ala  Asn Ala Ser Phe Gly  Asn Leu Lys 
    1310                 1315                 1320             


Pro Ile  Gly Phe Ala Thr Pro  Phe Gly Gln Met Gln  Ser Lys Asn 
    1325                 1330                 1335             


Gly Ile  Glu Leu Ser Glu Asn  Ala Phe Leu Ile His  Gln Ser Pro 
    1340                 1345                 1350             


Ser Gly  Leu Phe Glu His Lys  Ile Lys Leu Ser Asp  Leu 
    1355                 1360                 1365     


<210>  981
<211>  1366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  981

Met Thr Lys Leu Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser 
1               5                   10                  15      


Asn Ser Ile Gly Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg 
            20                  25                  30          


Leu Lys Gly Lys Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys 
        35                  40                  45              


Ala Ala Ala Tyr Arg Arg Ala Ser Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Asn Arg Trp Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His 
65                  70                  75                  80  


Ile Thr Pro Thr Asp Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu 
                85                  90                  95      


Ile Ser Pro Lys Asp Lys Ala Arg Tyr Lys Tyr Glu Lys Arg Leu Phe 
            100                 105                 110         


Asn Asp Arg Thr Asp Ala Glu Phe Tyr Gln Gln Tyr Pro Thr Met Tyr 
        115                 120                 125             


His Leu Arg Thr Arg Leu Leu Thr Asp Pro Ser Lys Ala Asp Val Arg 
    130                 135                 140                 


Glu Ile Tyr Phe Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe 
145                 150                 155                 160 


Leu Thr Pro Gly Tyr Ala Lys Asp Phe Asn Thr Asn Lys Val Ala Leu 
                165                 170                 175     


Asn Glu Ile Phe Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val Tyr Pro 
            180                 185                 190         


Asp Leu Asp Ile Thr Phe Asp Glu Asn Lys Val Asn Glu Phe Lys Thr 
        195                 200                 205             


Val Leu Leu Asn Glu Lys Ala Thr Pro Ser Asp Thr Gln Arg Ala Leu 
    210                 215                 220                 


Val Asn Leu Leu Leu Ala Lys Glu Gly Asp Arg Asp Ile Leu Lys Gln 
225                 230                 235                 240 


Gln Lys Gln Val Leu Thr Glu Phe Ala Lys Ala Val Val Gly Leu Lys 
                245                 250                 255     


Thr Lys Leu Asn Val Ala Leu Gly Thr Glu Val Asp Ser Ser Glu Ala 
            260                 265                 270         


Thr Ala Trp Asn Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp Ala Gly 
        275                 280                 285             


Ile Glu Ser Ala Met Thr Asp Glu Gly Thr Glu Ile Leu Asp Gln Ile 
    290                 295                 300                 


Arg Asp Leu Tyr Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly 
305                 310                 315                 320 


Lys Thr Leu Ser Gln Ala Lys Val Asp Asp Tyr Ala Gln His His Glu 
                325                 330                 335     


Asp Leu Glu Leu Phe Lys Asp Tyr Leu Lys Gln Leu Glu Asp Asp Gly 
            340                 345                 350         


Thr Ala Lys Ala Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp Gly Asp 
        355                 360                 365             


Asp Ala Lys Pro Phe Val Arg Glu Asp Phe Val Lys Ala Leu Thr Lys 
    370                 375                 380                 


Glu Val Thr Ala His Pro Asn Ala Lys Ser Pro Glu Leu Leu Glu Arg 
385                 390                 395                 400 


Leu Ala Gln Pro Asp Phe Met Leu Lys Gln Arg Asn Lys Ala Asn Gly 
                405                 410                 415     


Ala Ile Pro Val Gln Met Gln Gln Arg Glu Leu Asp Gln Ile Ile Lys 
            420                 425                 430         


Asn Gln Ala Val Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro Val Glu 
        435                 440                 445             


Lys His Arg Gln Ser Met Thr Tyr Gln Leu Asp Glu Leu Leu Asn Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Ala Lys Glu Gln Lys 
465                 470                 475                 480 


Ala Ala Gln Gly Gly Val Phe Ala Trp Met Val Arg Lys Asp Pro Asp 
                485                 490                 495     


Gly Asn Ile Thr Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala 
            500                 505                 510         


Ser Ala Asn Thr Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu 
        515                 520                 525             


Ile Gly Glu Asp Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Ser Tyr 
    530                 535                 540                 


Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn Asp Asn Lys Leu 
545                 550                 555                 560 


Ser Phe Asp Gln Lys Gln Gln Val Leu Gly Glu Leu Phe Glu Arg His 
                565                 570                 575     


Asn Thr Val Thr Ile Lys Gln Phe Val Asp Asn Leu Leu Ala His Gly 
            580                 585                 590         


Asp Tyr Asp Arg Ile Pro Glu Val Arg Gly Leu Ala Asp Glu Lys Arg 
        595                 600                 605             


Phe Leu Ser Ser Leu Ser Thr Tyr Asn Gln Leu Lys Gly Ile Leu His 
    610                 615                 620                 


Glu Ala Ile Asp Asp Ala Asp Lys Gln Thr Asp Ile Glu Asn Ile Ile 
625                 630                 635                 640 


Ala Trp Ser Thr Ile Phe Glu Asp Lys Ala Ile Phe Lys Thr Lys Leu 
                645                 650                 655     


Asn Glu Ile Ser Trp Leu Asn Ala Gly Met Ile Asn Ser Leu Ala Asn 
            660                 665                 670         


Ile Arg Tyr Arg Gly Trp Gly Gln Phe Ser His Lys Leu Leu Asn Glu 
        675                 680                 685             


Leu Thr Leu Ser Thr Gly Arg Thr Val Phe Glu Glu Leu Leu Ile Ser 
    690                 695                 700                 


Ser Arg Asn Leu Met Gln Ile Leu Thr Asp Glu Val Leu Lys Gln Lys 
705                 710                 715                 720 


Ile Ala Glu Leu Asn Ala His Glu Leu Lys Ala Ser Gly Ile Tyr Ala 
                725                 730                 735     


Ala Ile Asp Asp Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg 
            740                 745                 750         


Gln Val Leu Ser Val Ile Asp Asp Ile Thr Asn Ala Ala Gly Gln Ala 
        755                 760                 765             


Pro Asn Trp Leu Tyr Ile Glu Thr Ala Asp Gly Asp Gly Val Pro Gly 
    770                 775                 780                 


Lys Arg Thr Asn Ser Arg Gln Arg Lys Ile Gln Glu Ile Tyr Ala Asn 
785                 790                 795                 800 


Ala Ala Gln Glu Leu Ile Thr Gly Ser Val Arg Gly Glu Phe Glu Asp 
                805                 810                 815     


Lys Ile Ala Ser Arg Ala Asp Phe Thr Asp Arg Leu Val Leu Tyr Phe 
            820                 825                 830         


Met Gln Gly Gly Arg Asp Ile Tyr Thr Gly Glu Pro Leu Asn Ile Asp 
        835                 840                 845             


Asn Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu Ile 
    850                 855                 860                 


Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val His Pro Leu Val Asn 
865                 870                 875                 880 


Arg Glu Lys Asp Thr Ile Phe Ala Ser Thr Arg Phe Ala Lys Gln Met 
                885                 890                 895     


Asn Gly Met Trp Arg Gln Trp His Ala Ala Gly Leu Ile Ser Ser Arg 
            900                 905                 910         


Lys Leu Arg Asn Leu Gln Met Arg Pro Asp Glu Ile Asp Lys Tyr Ala 
        915                 920                 925             


Ser Gly Phe Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys 
    930                 935                 940                 


Leu Thr Glu Gln Ile Val Ala Asp Gln Tyr Pro Asp Thr Lys Ile Ile 
945                 950                 955                 960 


Ala Val Lys Ala Gly Leu Ser Ser Gln Leu Arg Lys Glu Leu Asp Phe 
                965                 970                 975     


Pro Lys Asn Arg Glu Val Asn His Tyr His His Ala Phe Asp Ala Phe 
            980                 985                 990         


Leu Ala Ala Arg Ile Gly Thr Tyr  Leu Leu Lys Arg Tyr  Pro Asn Leu 
        995                 1000                 1005             


Glu Pro  Phe Phe Thr Tyr Gly  Lys Phe Lys Lys Thr  Glu Val Lys 
    1010                 1015                 1020             


Lys Leu  Lys Ser Leu Lys Ser  Leu Lys Ser Phe Asn  Phe Ile Arg 
    1025                 1030                 1035             


Asp Met  Thr His Ala Lys Asp  Lys Ile Val Ala Lys  Glu Thr Gly 
    1040                 1045                 1050             


Glu Ile  Val Trp Asp Asn Ala  Ser Asp Ile Asn Glu  Leu Asp Arg 
    1055                 1060                 1065             


Ile Tyr  Asn Phe Lys Arg Met  Leu Ile Thr His Glu  Val Arg Phe 
    1070                 1075                 1080             


Glu Thr  Ala Ser Leu Phe Lys  Gln Thr Leu Tyr Ala  Ala Lys Asn 
    1085                 1090                 1095             


Ser Lys  Asn Arg Gly Gly Ser  Arg Gln Leu Ile Pro  Lys Lys Lys 
    1100                 1105                 1110             


Gly Tyr  Leu Val Asp Ile Tyr  Gly Gly Tyr Thr Gln  Glu Thr Gly 
    1115                 1120                 1125             


Ser Tyr  Leu Ser Val Val Arg  Leu Thr Lys Lys Ala  Met Tyr Ala 
    1130                 1135                 1140             


Val Val  Lys Val Ser Thr Arg  Asp Ala Ala Lys Leu  Ala Val Ala 
    1145                 1150                 1155             


Lys Ser  Ile Ser Glu Gln Lys  Glu Asn Glu Thr Leu  Lys Lys Ile 
    1160                 1165                 1170             


Ile Asp  Gly Lys Leu Ser Lys  Thr Ser Lys Lys Gly  Lys Thr Thr 
    1175                 1180                 1185             


His Gln  Leu Phe Glu Ile Val  Leu Pro Arg Val Gly  Gln Lys Thr 
    1190                 1195                 1200             


Leu Phe  Lys Asn Ser Lys Tyr  Asn Gln Phe Leu Val  Asn Ser Asp 
    1205                 1210                 1215             


Thr Tyr  Met His Asn Tyr Gln  Glu Leu Trp Met Pro  Arg Glu Tyr 
    1220                 1225                 1230             


Gln Arg  Met Trp Lys Asp Ile  Leu Leu Ser Asn His  Gly Asp Ala 
    1235                 1240                 1245             


Gln Ile  Glu Gly Gln Leu Asp  Gln Ile Phe Lys Phe  Ile Val Ser 
    1250                 1255                 1260             


Gln Val  Asn Ser Tyr Phe Asn  Leu Tyr Asp Ile Asn  Gln Phe Arg 
    1265                 1270                 1275             


Lys Lys  Ile Ala Asp Ala Thr  Asp Lys Phe Ala Lys  Leu Pro Thr 
    1280                 1285                 1290             


His Asp  Thr Asp Asn Ala Arg  Gly Lys Ile Ala Thr  Ile Gly Gln 
    1295                 1300                 1305             


Leu Leu  Ile Gly Leu Gln Ala  Asn Ala Met Arg Ser  Asp Leu Arg 
    1310                 1315                 1320             


Asn Leu  Asp Ile Lys Thr Pro  Phe Gly Leu Leu Gln  Ile Gly Thr 
    1325                 1330                 1335             


Gly Ile  Thr Leu Asp Leu Asp  Thr Ser Ile Ile Tyr  Gln Ser Pro 
    1340                 1345                 1350             


Thr Gly  Leu Phe Glu Arg Glu  Val Pro Leu Thr Asp  Leu 
    1355                 1360                 1365     


<210>  982
<211>  1366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  982

Met Thr Lys Asp Tyr Ser Leu Gly Leu Asp Ile Gly Thr Gly Ser Val 
1               5                   10                  15      


Gly Phe Ala Ala Leu Asp Thr Asn Tyr Arg Leu Leu Arg Ala Lys Gly 
            20                  25                  30          


Asn Asn Val Ile Gly Val Arg Leu Phe Thr Pro Ala Glu Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Gly Phe Arg Thr Ala Arg Arg Arg Leu Ser Arg Arg Arg 
    50                  55                  60                  


Trp Arg Leu Gly Ala Leu Asn Gly Ile Phe Ala Pro Glu Leu Ala Lys 
65                  70                  75                  80  


Val Asp Pro Asn Phe Leu His Arg Leu Lys Tyr Ala Trp Val His Pro 
                85                  90                  95      


Ala Asp Gln Gln Asn Lys Gly Lys Tyr Ser Ala Ala Pro Leu Phe Asn 
            100                 105                 110         


Asp Ala Ala Leu Asp Arg Gln Phe His Asp Asp Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Leu Lys Leu Met Thr Asp Thr Ser Gln His Asp Leu Arg 
    130                 135                 140                 


Glu Val Tyr Leu Ala Ile His His Ile Ile Lys Phe Arg Gly Asn Phe 
145                 150                 155                 160 


Leu Arg Gly Glu Gly Pro Ile Asn Ala Ser Glu Ala Phe Asp Ile Asn 
                165                 170                 175     


Thr Phe Ile Asp Gln Met Thr Ala Phe Tyr Asp Gln Ala Phe Pro Asp 
            180                 185                 190         


Phe Lys Ile Val Asp Gln Val Asp Gln Gln Ile Ile Phe Lys Gly Leu 
        195                 200                 205             


Thr Asp Met Thr Leu Ser Pro Ala Asp Arg Ala Asn Lys Ala Leu Met 
    210                 215                 220                 


Gly Phe Asn Asn Ala Ala Gln Gln Pro Asn Lys Arg Ile Phe Lys Ala 
225                 230                 235                 240 


Ile Leu Ser Gly Leu Val Gly Asn Gln Val Asp Leu Met Ala Ile Phe 
                245                 250                 255     


Gln Leu Asp Gly Ile Pro Lys Glu Asp Gln Lys Glu Phe Lys Phe Lys 
            260                 265                 270         


Phe Val Asp Asn Asp Ile Glu Glu Lys Leu Ala Ala Leu Gly Asp Asn 
        275                 280                 285             


Pro Ala Leu Ser Asp Glu Gln Asn Glu Phe Leu Thr Thr Leu Tyr Ala 
    290                 295                 300                 


Ala Phe Ser Gly Leu Thr Leu Lys Gly Leu Leu Gly Asp Ala Thr Ser 
305                 310                 315                 320 


Ile Ser Ala Ala Met Val Ala Arg Tyr Ala Ser His Arg Thr Asp Trp 
                325                 330                 335     


Thr Tyr Ile Lys Asp His Val Leu Thr Pro Gln Asn Asn Arg Glu Leu 
            340                 345                 350         


Arg Leu Ala Tyr Ala Asp Leu Met Thr Asp Asn Glu Asp Val Arg Leu 
        355                 360                 365             


Lys Ala Gly Lys Lys Phe Ala Lys Ala Ile Glu Ala Ala Asp Ile Pro 
    370                 375                 380                 


Ala Ala Lys Lys Ser Glu Phe Ser Leu Lys Ile Asp Asn Asn Glu Phe 
385                 390                 395                 400 


Leu Pro Arg Gln Arg Thr Lys Ala Asn Gly Thr Ile Pro His Gln Leu 
                405                 410                 415     


His Leu Asn Glu Leu Gln Gln Ile Ile Glu Asn Gln Gly Gln Tyr Tyr 
            420                 425                 430         


Pro Phe Leu Lys Ala Thr Phe Glu Gln Asp Gly His Thr Leu Asn Lys 
        435                 440                 445             


Ile Glu Gly Leu Val Lys Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu 
    450                 455                 460                 


Val Pro Lys His Lys Val Ala Gly Asn Asn Ala Asn His Trp Met Val 
465                 470                 475                 480 


Pro Lys Glu Pro Gly Ala Ile Thr Pro Trp Asn Tyr Thr Glu Lys Val 
                485                 490                 495     


Asp Arg Asp Lys Ser Gly Asn Glu Phe Ile Arg Arg Met Thr Gly Thr 
            500                 505                 510         


Asp Thr Tyr Leu Leu Gly Glu Pro Thr Leu Pro Lys Asn Ser Leu Val 
        515                 520                 525             


Tyr Gln Glu Phe Asn Val Leu Gln Glu Leu Asn Asn Leu Arg Ile Glu 
    530                 535                 540                 


Lys Arg Arg Leu Asp Ile Ala Val Lys Gln Asp Ile Phe Glu His Val 
545                 550                 555                 560 


Phe Lys Thr Asn Lys Thr Val Thr Val Lys Arg Ile Glu Glu Tyr Leu 
                565                 570                 575     


Thr Thr Glu Phe Gly Ser Pro Val Thr Leu Ser Gly Phe Ala Asn Val 
            580                 585                 590         


Asn Glu Arg Lys Leu Asn Ser Ser Leu Ser Thr Tyr His Tyr Phe Val 
        595                 600                 605             


Gly Ile Phe Gly Ala Glu Phe Val Asp Asp Pro Lys Asn Leu Thr Leu 
    610                 615                 620                 


Leu Glu Gln Ile Val Glu Leu Gln Thr Val Phe Glu Asp Arg Lys Ala 
625                 630                 635                 640 


Leu Gln Arg Gln Leu Lys Leu Arg Pro Glu Leu Ser Glu Lys Gln Val 
                645                 650                 655     


Lys Gln Leu Ala Arg Thr His Tyr Thr Gly Trp Gly Arg Leu Ser Gln 
            660                 665                 670         


Lys Leu Leu Thr Ser Lys Ile Val His Val Ala Leu Glu Gly Glu Arg 
        675                 680                 685             


Glu Pro Gly Gln Tyr Ser Ile Leu Glu Thr Leu Tyr Gly Thr Gln Met 
    690                 695                 700                 


Asn Phe Met Glu Ile Val Asn Asp Met Lys Asp Arg Tyr Gly Val Lys 
705                 710                 715                 720 


Ser Trp Leu Asp Gln Glu Asn Ser Gly Ser Glu Asn Asn Leu Ser Leu 
                725                 730                 735     


Lys Gln Arg Ile Leu Asp Met Pro Gly Pro Lys Asn Ile Lys Arg Gly 
            740                 745                 750         


Ile Asn Gln Ala Phe His Ile Ile Asp Glu Ile Thr Arg Ala Val Gly 
        755                 760                 765             


Asn Ala Pro Ser Arg Ile Tyr Ile Glu Phe Ala Arg Glu Ser Gln Gln 
    770                 775                 780                 


Ser Asn Leu Thr Asn Ala Arg Val Asn Arg Leu Lys Gln Leu Tyr Ser 
785                 790                 795                 800 


Asn Gln Ala Leu Lys Lys Glu Phe Ala Lys Leu Ala Ser Glu Ile Lys 
                805                 810                 815     


Asp Leu Thr Lys Glu Gln Leu Lys Asp Asp Arg Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Leu Gln Gln Gly Lys Asp Met Tyr Thr Glu Thr Asp Ile Asp Ile Thr 
        835                 840                 845             


Lys Ile Ser Ser Asn Tyr Gln Ile Asp His Ile Ile Pro Gln Ala Tyr 
    850                 855                 860                 


Thr Lys Asn Asn Ser Leu Asp Asn Arg Val Leu Val Asn Ser Glu Met 
865                 870                 875                 880 


Asn Ala Arg Lys Ser Asp Ser Pro Thr Phe Leu Pro Ser Leu Ile Gln 
                885                 890                 895     


Glu Arg Lys Ala Trp Trp Arg Gln Leu Arg Asp Thr Gly Leu Met Ser 
            900                 905                 910         


Thr Ile Lys Tyr Asn Asn Leu Thr Arg Thr Ser Asp Asp Phe Ser Ala 
        915                 920                 925             


Asp Gln Lys Glu Arg Phe Ile Ala Arg Ser Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Val Ile Lys Asn Val Ala Thr Leu Ile Lys Glu Tyr Tyr Asp Asp Ser 
945                 950                 955                 960 


Gln Val Glu Ala Leu Pro Ser Glu Ile Thr Ala Glu Met Arg Arg Tyr 
                965                 970                 975     


Leu Asn Val Arg Lys Asn Arg Asp Ile Asn Asp Tyr His His Ala His 
            980                 985                 990         


Asp Ala Leu Leu Phe Ala Thr Ala  Gly Glu Phe Ser Arg  Lys Val Gly 
        995                 1000                 1005             


Ile Met  Glu Lys Gly Arg Val  Ser Asp Gly Ala Gly  Asn Ile Tyr 
    1010                 1015                 1020             


Asn Arg  Tyr Thr Lys Asp Ile  Ile Gln Arg Ala Arg  Ala Lys Ala 
    1025                 1030                 1035             


Gly Gln  Asp Lys Lys Val Arg  Pro Phe Thr Phe Ile  Val Gly Ser 
    1040                 1045                 1050             


Met Ala  Asn Pro Asp Thr Lys  Phe Gln Thr Asn Gln  Thr Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Thr Pro Glu  Asp Val Ala Tyr Leu  Arg Lys Val 
    1070                 1075                 1080             


Ile Asp  Tyr Lys Lys Met Leu  Val Thr Lys Met Thr  Leu Asp Gln 
    1085                 1090                 1095             


Thr Gly  Thr Leu Tyr Asn Glu  Thr Arg Tyr Gly Ser  Gly Asp Lys 
    1100                 1105                 1110             


His Val  Ala Val Ala Leu Ser  Lys Asp Lys Ala Ala  Asp Leu Tyr 
    1115                 1120                 1125             


Gly Gly  Phe Gly Ser Leu Lys  Pro Ala Tyr Ala Ala  Leu Val Tyr 
    1130                 1135                 1140             


Ser Lys  Lys Lys Phe Arg Leu  Val Asn Val Leu Arg  Leu Trp Leu 
    1145                 1150                 1155             


Pro Glu  Ile Lys Lys Asn Ala  Ser Val Leu Glu Arg  Lys Val Gln 
    1160                 1165                 1170             


Ser Val  Leu Gly Asp Asp Asn  Ala Lys Val Ile Arg  Ala His Val 
    1175                 1180                 1185             


Pro Cys  Gly Gln Leu Leu Ile  Lys Asp Gly Ala Leu  Val Thr Val 
    1190                 1195                 1200             


Ser Ser  Ala Thr Glu Leu His  Asn Phe Arg Gln Leu  Trp Leu Pro 
    1205                 1210                 1215             


Lys Ala  Asp Tyr Gln Asp Ile  Ser Ile Leu Leu Gly  Ala Pro Asp 
    1220                 1225                 1230             


Lys Val  Thr Ala Glu Asn Arg  Leu Gln Ala Glu Ser  His Phe Asp 
    1235                 1240                 1245             


Asp Val  Asp Gln Arg Phe Glu  Ser Leu Leu Lys Asp  Val Ile Asp 
    1250                 1255                 1260             


Gln Ala  Arg Gln Tyr Tyr Pro  Leu Lys Thr His Gln  Thr Ser Leu 
    1265                 1270                 1275             


Glu Lys  Leu Ile Gly Lys Ile  Gly Lys Phe Asp Glu  Leu Lys Phe 
    1280                 1285                 1290             


Ala Gln  Lys Gln Gln Val Phe  Ser Arg Leu Leu Ser  Ala Leu His 
    1295                 1300                 1305             


Ala Asn  Ala Thr Asn Ser Asp  Leu Lys Gln Ile Gly  Leu Ser Thr 
    1310                 1315                 1320             


Ala Trp  Gly Arg Leu Ile Tyr  Pro Lys Gly Val Ser  Leu Thr Asp 
    1325                 1330                 1335             


Glu Asp  Lys Leu Ile Asn Ser  Ser Phe Thr Gly Leu  Phe Asn Asp 
    1340                 1345                 1350             


Val Met  Ser Ile Lys Glu Phe  His Asp Phe Ser Ile  Asn 
    1355                 1360                 1365     


<210>  983
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  983

Met Lys Lys Gln Lys Phe Ser Asp Tyr Tyr Leu Gly Phe Asp Ile Gly 
1               5                   10                  15      


Thr Asn Ser Val Gly Trp Cys Ile Thr Asp Leu Asp Tyr Asn Val Leu 
            20                  25                  30          


Arg Phe Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Glu Glu Ala 
        35                  40                  45              


Lys Thr Ala Ala Glu Arg Arg Val Gln Arg Asn Ser Arg Arg Arg Leu 
    50                  55                  60                  


Lys Arg Arg Lys Trp Arg Leu Asn Leu Leu Glu Glu Ile Phe Ser Asn 
65                  70                  75                  80  


Glu Ile Leu Lys Ile Asp Ser Asn Phe Phe Arg Arg Leu Lys Glu Ser 
                85                  90                  95      


Ser Leu Trp Leu Glu Asp Lys Ser Ser Lys Glu Lys Phe Thr Leu Phe 
            100                 105                 110         


Asn Asp Asp Asn Tyr Lys Asp Tyr Asp Phe Tyr Lys Gln Tyr Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Asn Glu Leu Ile Lys Asn Pro Glu Lys Lys Asp 
    130                 135                 140                 


Ile Arg Leu Val Tyr Leu Ala Ile His Ser Ile Phe Lys Ser Arg Gly 
145                 150                 155                 160 


His Phe Leu Phe Glu Gly Gln Asn Leu Lys Glu Ile Lys Asn Phe Glu 
                165                 170                 175     


Thr Leu Tyr Asn Asn Leu Ile Ala Phe Leu Glu Asp Asn Gly Ile Asn 
            180                 185                 190         


Lys Ile Ile Asp Lys Asn Asn Ile Glu Lys Leu Glu Lys Ile Val Cys 
        195                 200                 205             


Asp Ser Lys Lys Gly Leu Lys Asp Lys Glu Lys Glu Phe Lys Glu Ile 
    210                 215                 220                 


Phe Asn Ser Asp Lys Gln Leu Val Ala Ile Phe Lys Leu Ser Val Gly 
225                 230                 235                 240 


Ser Ser Val Ser Leu Asn Asp Leu Phe Asp Thr Asp Glu Tyr Lys Lys 
                245                 250                 255     


Gly Glu Val Glu Lys Glu Lys Ile Ser Phe Arg Glu Gln Ile Tyr Glu 
            260                 265                 270         


Asp Asp Lys Pro Ile Tyr Tyr Ser Ile Leu Gly Glu Lys Ile Glu Leu 
        275                 280                 285             


Leu Asp Ile Ala Lys Ser Phe Tyr Asp Phe Met Val Leu Asn Asn Ile 
    290                 295                 300                 


Leu Ala Asp Ser Gln Tyr Ile Ser Glu Ala Lys Val Lys Leu Tyr Glu 
305                 310                 315                 320 


Glu His Lys Lys Asp Leu Lys Asn Leu Lys Tyr Ile Ile Arg Lys Tyr 
                325                 330                 335     


Asn Lys Gly Asn Tyr Asp Lys Leu Phe Lys Asp Lys Asn Glu Asn Asn 
            340                 345                 350         


Tyr Ser Ala Tyr Ile Gly Leu Asn Lys Glu Lys Gly Lys Lys Glu Val 
        355                 360                 365             


Val Glu Lys Ser Arg Leu Lys Ile Asp Asp Leu Ile Lys Val Ile Lys 
    370                 375                 380                 


Gly Tyr Leu Pro Lys Pro Glu Arg Ile Glu Glu Lys Asp Lys Thr Val 
385                 390                 395                 400 


Phe Asn Glu Ile Leu Ser Lys Ile Glu Leu Lys Thr Ile Leu Pro Lys 
                405                 410                 415     


Gln Arg Ile Ser Asp Asn Gly Thr Leu Pro Tyr Gln Ile His Glu Val 
            420                 425                 430         


Glu Leu Glu Lys Ile Leu Glu Asn Gln Ser Lys Tyr Tyr Asp Phe Leu 
        435                 440                 445             


Asn Tyr Glu Glu Asn Gly Val Ser Thr Lys Asp Lys Leu Leu Lys Thr 
    450                 455                 460                 


Phe Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Ser Tyr His 
465                 470                 475                 480 


Lys Asp Lys Gly Gly Asn Ser Trp Ile Val Arg Lys Glu Glu Gly Lys 
                485                 490                 495     


Ile Leu Pro Trp Asn Phe Glu Gln Lys Val Asp Ile Glu Lys Ser Ala 
            500                 505                 510         


Glu Glu Phe Ile Lys Arg Met Thr Asn Lys Cys Thr Tyr Leu Asn Gly 
        515                 520                 525             


Glu Asp Val Ile Pro Lys Asp Thr Phe Leu Tyr Ser Glu Tyr Val Ile 
    530                 535                 540                 


Leu Asn Glu Leu Asn Lys Val Gln Val Asn Asp Glu Phe Leu Asn Glu 
545                 550                 555                 560 


Glu Asn Lys Arg Lys Ile Ile Asp Glu Leu Phe Lys Glu Asn Lys Lys 
                565                 570                 575     


Val Ser Glu Lys Lys Phe Lys Glu Tyr Leu Leu Val Lys Gln Ile Val 
            580                 585                 590         


Asp Gly Thr Ile Glu Leu Lys Gly Val Lys Asp Ser Phe Asn Ser Asn 
        595                 600                 605             


Tyr Ile Ser Tyr Ile Arg Phe Lys Asp Ile Phe Gly Glu Lys Leu Asn 
    610                 615                 620                 


Leu Asp Ile Tyr Lys Glu Ile Ser Glu Lys Ser Ile Leu Trp Lys Cys 
625                 630                 635                 640 


Leu Tyr Gly Asp Asp Lys Lys Ile Phe Glu Lys Lys Ile Lys Asn Glu 
                645                 650                 655     


Tyr Gly Asp Ile Leu Thr Lys Asp Glu Ile Lys Lys Ile Asn Thr Phe 
            660                 665                 670         


Lys Phe Asn Asn Trp Gly Arg Leu Ser Glu Lys Leu Leu Thr Gly Ile 
        675                 680                 685             


Glu Phe Ile Asn Leu Glu Thr Gly Glu Cys Tyr Ser Ser Val Met Asp 
    690                 695                 700                 


Ala Leu Arg Arg Thr Asn Tyr Asn Leu Met Glu Leu Leu Ser Ser Lys 
705                 710                 715                 720 


Phe Thr Leu Gln Glu Ser Ile Asn Asn Glu Asn Lys Glu Met Asn Glu 
                725                 730                 735     


Ala Ser Tyr Arg Asp Leu Ile Glu Glu Ser Tyr Val Ser Pro Ser Leu 
            740                 745                 750         


Lys Arg Ala Ile Phe Gln Thr Leu Lys Ile Tyr Glu Glu Ile Arg Lys 
        755                 760                 765             


Ile Thr Gly Arg Val Pro Lys Lys Val Phe Ile Glu Met Ala Arg Gly 
    770                 775                 780                 


Gly Asp Glu Ser Met Lys Asn Lys Lys Ile Pro Ala Arg Gln Glu Gln 
785                 790                 795                 800 


Leu Lys Lys Leu Tyr Asp Ser Cys Gly Asn Asp Ile Ala Asn Phe Ser 
                805                 810                 815     


Ile Asp Ile Lys Glu Met Lys Asn Ser Leu Ile Ser Tyr Asp Asn Asn 
            820                 825                 830         


Ser Leu Arg Gln Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Phe Gly Lys 
        835                 840                 845             


Cys Met Tyr Thr Gly Arg Glu Ile Asp Leu Asp Arg Leu Leu Gln Asn 
    850                 855                 860                 


Asn Asp Thr Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Lys Val Ile 
865                 870                 875                 880 


Lys Asp Asp Ser Phe Asp Asn Leu Val Leu Val Leu Lys Asn Glu Asn 
                885                 890                 895     


Ala Glu Lys Ser Asn Glu Tyr Pro Val Lys Lys Glu Ile Gln Glu Lys 
            900                 905                 910         


Met Lys Ser Phe Trp Arg Phe Leu Lys Glu Lys Asn Phe Ile Ser Asp 
        915                 920                 925             


Glu Lys Tyr Lys Arg Leu Thr Gly Lys Asp Asp Phe Glu Leu Arg Gly 
    930                 935                 940                 


Phe Met Ala Arg Gln Leu Val Asn Val Arg Gln Thr Thr Lys Glu Val 
945                 950                 955                 960 


Gly Lys Ile Leu Gln Gln Ile Glu Pro Glu Ile Lys Ile Val Tyr Ser 
                965                 970                 975     


Lys Ala Glu Ile Ala Ser Ser Phe Arg Glu Met Phe Asp Phe Ile Lys 
            980                 985                 990         


Val Arg Glu Leu Asn Asp Thr His  His Ala Lys Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ile Val  Ala Gly Asn Val Tyr  Asn Thr Lys Phe Thr  Glu Lys Pro 
    1010                 1015                 1020             


Tyr Arg  Tyr Leu Gln Glu Ile  Lys Glu Asn Tyr Asp  Val Lys Lys 
    1025                 1030                 1035             


Ile Tyr  Asn Tyr Asp Ile Lys  Asn Ala Trp Asp Lys  Glu Asn Ser 
    1040                 1045                 1050             


Leu Glu  Ile Val Lys Lys Asn  Met Glu Lys Asn Thr  Val Asn Ile 
    1055                 1060                 1065             


Thr Arg  Phe Ile Lys Glu Glu  Lys Gly Gln Leu Phe  Asp Leu Asn 
    1070                 1075                 1080             


Pro Ile  Lys Lys Gly Glu Thr  Ser Asn Glu Ile Ile  Ser Ile Lys 
    1085                 1090                 1095             


Pro Lys  Val Tyr Asn Gly Lys  Asp Asp Lys Leu Asn  Glu Lys Tyr 
    1100                 1105                 1110             


Gly Tyr  Tyr Lys Ser Leu Asn  Pro Ala Tyr Phe Leu  Tyr Val Glu 
    1115                 1120                 1125             


His Lys  Glu Lys Asn Lys Arg  Ile Lys Ser Phe Glu  Arg Val Asn 
    1130                 1135                 1140             


Leu Val  Asp Val Asn Asn Ile  Lys Asp Glu Lys Ser  Leu Val Lys 
    1145                 1150                 1155             


Tyr Leu  Ile Glu Asn Lys Lys  Leu Val Glu Pro Arg  Val Ile Lys 
    1160                 1165                 1170             


Lys Val  Tyr Lys Arg Gln Val  Ile Leu Ile Asn His  Tyr Pro Tyr 
    1175                 1180                 1185             


Ser Ile  Val Thr Leu Asp Ser  Asn Lys Leu Met Asp  Phe Glu Asn 
    1190                 1195                 1200             


Leu Lys  Pro Leu Phe Leu Glu  Asn Lys Tyr Glu Lys  Ile Leu Lys 
    1205                 1210                 1215             


Asn Val  Ile Lys Phe Leu Glu  Asp Asn Gln Gly Lys  Ser Glu Glu 
    1220                 1225                 1230             


Asn Tyr  Lys Phe Ile Tyr Leu  Lys Lys Lys Asp Arg  Tyr Glu Lys 
    1235                 1240                 1245             


Asn Glu  Thr Leu Glu Ser Val  Lys Asp Arg Tyr Asn  Leu Glu Phe 
    1250                 1255                 1260             


Asn Glu  Met Tyr Asp Lys Phe  Leu Glu Lys Leu Asp  Ser Lys Asp 
    1265                 1270                 1275             


Tyr Lys  Asn Tyr Met Asn Asn  Lys Lys Tyr Gln Glu  Leu Leu Asp 
    1280                 1285                 1290             


Val Lys  Glu Lys Phe Ile Lys  Leu Asn Leu Phe Asp  Lys Ala Phe 
    1295                 1300                 1305             


Thr Leu  Lys Ser Phe Leu Asp  Leu Phe Asn Arg Lys  Thr Met Ala 
    1310                 1315                 1320             


Asp Phe  Ser Lys Val Gly Leu  Thr Lys Tyr Leu Gly  Lys Ile Gln 
    1325                 1330                 1335             


Lys Ile  Ser Ser Asn Val Leu  Ser Lys Asn Glu Leu  Tyr Leu Leu 
    1340                 1345                 1350             


Glu Glu  Ser Val Thr Gly Leu  Phe Val Lys Lys Ile  Lys Leu 
    1355                 1360                 1365         


<210>  984
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  984

Met Lys Lys Gln Lys Phe Ser Asp Tyr Tyr Leu Gly Phe Asp Ile Gly 
1               5                   10                  15      


Thr Asn Ser Val Gly Trp Cys Val Thr Asp Leu Asp Tyr Asn Val Leu 
            20                  25                  30          


Arg Phe Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Asp Glu Ala 
        35                  40                  45              


Lys Thr Ala Ala Glu Arg Arg Val Gln Arg Asn Ser Arg Arg Arg Leu 
    50                  55                  60                  


Lys Arg Arg Lys Trp Arg Leu Asn Leu Leu Glu Glu Ile Phe Ser Asp 
65                  70                  75                  80  


Glu Ile Met Lys Ile Asp Ser Asn Phe Phe Arg Arg Leu Lys Glu Ser 
                85                  90                  95      


Ser Leu Trp Leu Glu Asp Lys Asn Ser Lys Glu Lys Phe Thr Leu Phe 
            100                 105                 110         


Asn Asp Asp Asn Tyr Lys Asp Tyr Asp Phe Tyr Lys Gln Tyr Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Asp Glu Leu Ile Lys Asn Pro Glu Lys Lys Asp 
    130                 135                 140                 


Ile Arg Leu Ile Tyr Leu Ala Leu His Ser Ile Phe Lys Ser Arg Gly 
145                 150                 155                 160 


His Phe Leu Phe Glu Gly Gln Asn Leu Lys Glu Ile Lys Asn Phe Glu 
                165                 170                 175     


Thr Leu Tyr Asn Asn Leu Ile Ser Phe Leu Glu Asp Asn Gly Ile Asn 
            180                 185                 190         


Lys Ser Ile Asp Lys Asp Asn Ile Lys Lys Leu Glu Lys Ile Ile Cys 
        195                 200                 205             


Asp Ser Gly Lys Gly Leu Lys Asp Lys Glu Lys Glu Phe Lys Glu Ile 
    210                 215                 220                 


Phe Asn Ser Asp Lys Gln Leu Val Ala Ile Phe Lys Leu Ser Val Gly 
225                 230                 235                 240 


Ser Ser Val Ser Leu Asn Asp Leu Phe Asp Thr Asp Glu Tyr Lys Lys 
                245                 250                 255     


Glu Glu Val Glu Lys Glu Lys Ile Ser Phe Arg Glu Gln Ile Tyr Glu 
            260                 265                 270         


Asp Asp Lys Pro Ile Tyr Tyr Ser Ile Leu Gly Glu Lys Ile Glu Leu 
        275                 280                 285             


Leu Asp Ile Ala Lys Ser Phe Tyr Asp Phe Met Val Leu Asn Asn Ile 
    290                 295                 300                 


Leu Ser Asp Ser Asn Tyr Ile Ser Glu Ala Lys Val Lys Leu Tyr Glu 
305                 310                 315                 320 


Glu His Lys Lys Asp Leu Lys Asn Leu Lys Tyr Ile Ile Arg Lys Tyr 
                325                 330                 335     


Asn Lys Glu Asn Tyr Asp Lys Leu Phe Lys Asp Lys Asn Glu Asn Asn 
            340                 345                 350         


Tyr Pro Ala Tyr Ile Gly Leu Asn Lys Glu Lys Gly Lys Lys Glu Val 
        355                 360                 365             


Val Glu Lys Ser Gly Leu Lys Ile Asp Asp Leu Ile Lys Val Ile Lys 
    370                 375                 380                 


Gly Tyr Leu Pro Lys Pro Glu Arg Ile Glu Glu Lys Asp Lys Thr Ile 
385                 390                 395                 400 


Phe Asn Glu Ile Leu Asn Lys Ile Glu Leu Lys Thr Ile Leu Pro Lys 
                405                 410                 415     


Gln Arg Ile Ser Asp Asn Gly Thr Leu Pro Tyr Gln Ile His Glu Val 
            420                 425                 430         


Glu Leu Glu Lys Ile Leu Glu Asn Gln Ser Lys Tyr Tyr Asp Phe Leu 
        435                 440                 445             


Asn Tyr Glu Glu Asn Gly Val Ser Thr Lys Asp Lys Leu Leu Lys Thr 
    450                 455                 460                 


Phe Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Ser Tyr His 
465                 470                 475                 480 


Lys Asp Lys Gly Gly Asn Ser Trp Ile Val Arg Lys Glu Glu Gly Lys 
                485                 490                 495     


Ile Leu Pro Trp Asn Phe Glu Gln Lys Val Asp Ile Glu Lys Ser Ala 
            500                 505                 510         


Glu Glu Phe Ile Lys Arg Met Thr Asn Lys Cys Thr Tyr Leu Asn Gly 
        515                 520                 525             


Glu Asp Val Ile Pro Lys Asp Ser Phe Leu Tyr Ser Glu Tyr Ile Ile 
    530                 535                 540                 


Leu Asn Glu Leu Asn Lys Val Gln Val Asn Asp Glu Phe Leu Asn Glu 
545                 550                 555                 560 


Glu Asn Lys Arg Lys Ile Ile Asp Glu Leu Phe Lys Glu Asn Lys Lys 
                565                 570                 575     


Val Ser Glu Lys Lys Phe Lys Glu Tyr Leu Leu Val Asn Gln Ile Ala 
            580                 585                 590         


Asn Arg Thr Val Glu Leu Lys Gly Ile Lys Asp Ser Phe Asn Ser Asn 
        595                 600                 605             


Tyr Val Ser Tyr Ile Lys Phe Lys Asp Ile Phe Gly Glu Lys Leu Asn 
    610                 615                 620                 


Leu Asp Ile Tyr Lys Glu Ile Ser Glu Lys Ser Ile Leu Trp Lys Cys 
625                 630                 635                 640 


Leu Tyr Gly Asp Asp Lys Lys Ile Phe Glu Lys Lys Ile Lys Asn Glu 
                645                 650                 655     


Tyr Gly Asp Ile Leu Asn Lys Asp Glu Ile Lys Lys Ile Asn Ser Phe 
            660                 665                 670         


Lys Phe Asn Thr Trp Gly Arg Leu Ser Glu Lys Leu Leu Thr Gly Ile 
        675                 680                 685             


Glu Phe Ile Asn Leu Glu Thr Gly Glu Cys Tyr Ser Ser Val Met Glu 
    690                 695                 700                 


Ala Leu Arg Arg Thr Asn Tyr Asn Leu Met Glu Leu Leu Ser Ser Lys 
705                 710                 715                 720 


Phe Thr Leu Gln Glu Ser Ile Asp Asn Gly Asn Lys Glu Met Asn Glu 
                725                 730                 735     


Val Ser Tyr Arg Asp Leu Ile Glu Glu Ser Tyr Val Ser Pro Ser Leu 
            740                 745                 750         


Lys Arg Ala Ile Leu Gln Thr Leu Lys Ile Tyr Glu Glu Ile Lys Lys 
        755                 760                 765             


Ile Thr Gly Arg Val Pro Lys Lys Val Phe Ile Glu Met Ala Arg Gly 
    770                 775                 780                 


Gly Asp Glu Thr Met Lys Asn Lys Lys Ile Pro Ala Arg Gln Glu Gln 
785                 790                 795                 800 


Leu Lys Lys Leu Tyr Asp Ser Cys Gly Asn Asp Ile Ala Asn Phe Ser 
                805                 810                 815     


Ile Asp Ile Lys Lys Met Lys Ser Ser Leu Ser Ser Tyr Asp Asn Asn 
            820                 825                 830         


Ser Leu Arg Gln Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Phe Gly Lys 
        835                 840                 845             


Cys Met Tyr Thr Gly Arg Glu Ile Asp Leu Asp Arg Leu Leu Gln Asn 
    850                 855                 860                 


Asn Asp Thr Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Lys Val Ile 
865                 870                 875                 880 


Lys Asp Asp Ser Phe Asp Asn Leu Val Leu Val Leu Lys Asn Glu Asn 
                885                 890                 895     


Ala Glu Lys Ser Asn Glu Tyr Pro Val Lys Lys Glu Ile Gln Glu Lys 
            900                 905                 910         


Met Lys Ser Phe Trp Arg Phe Leu Lys Glu Lys Asn Phe Ile Ser Asp 
        915                 920                 925             


Glu Lys Tyr Lys Arg Leu Thr Gly Lys Asp Asp Phe Glu Leu Arg Gly 
    930                 935                 940                 


Phe Met Ala Arg Gln Leu Val Asn Val Arg Gln Thr Thr Lys Glu Val 
945                 950                 955                 960 


Gly Lys Ile Leu Gln Gln Ile Glu Pro Glu Ile Lys Ile Val Tyr Ser 
                965                 970                 975     


Lys Ala Glu Ile Ala Ser Ser Phe Arg Glu Met Phe Asp Phe Ile Lys 
            980                 985                 990         


Val Arg Glu Leu Asn Asp Thr His  His Ala Lys Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ile Val  Ala Gly Asn Val Tyr  Asn Thr Lys Phe Thr  Glu Lys Pro 
    1010                 1015                 1020             


Tyr Arg  Tyr Leu Gln Glu Ile  Lys Glu Asn Tyr Asp  Val Lys Lys 
    1025                 1030                 1035             


Ile Tyr  Asn Tyr Asp Ile Lys  Asn Ala Trp Asp Lys  Glu Asn Ser 
    1040                 1045                 1050             


Leu Glu  Ile Val Lys Lys Asn  Met Lys Lys Asn Thr  Val Asn Ile 
    1055                 1060                 1065             


Thr Arg  Phe Ile Lys Glu Glu  Lys Gly Gln Leu Phe  Asp Leu Asn 
    1070                 1075                 1080             


Pro Ile  Lys Lys Gly Glu Thr  Ser Asn Glu Ile Ile  Ser Ile Lys 
    1085                 1090                 1095             


Pro Lys  Leu Tyr Asn Gly Lys  Glu Glu Lys Leu Asn  Glu Lys Tyr 
    1100                 1105                 1110             


Gly Tyr  Tyr Lys Ser Leu Asn  Pro Ala Tyr Phe Leu  Tyr Val Glu 
    1115                 1120                 1125             


His Lys  Glu Lys Asn Lys Arg  Ile Lys Ser Phe Glu  Arg Val Asn 
    1130                 1135                 1140             


Leu Val  Asp Val Asn Asn Ile  Lys Asp Glu Lys Ser  Leu Val Lys 
    1145                 1150                 1155             


Tyr Leu  Ile Glu Asn Lys Lys  Leu Val Glu Pro Arg  Leu Ile Lys 
    1160                 1165                 1170             


Lys Val  Tyr Lys Arg Gln Val  Ile Leu Ile Asn Asp  Tyr Pro Tyr 
    1175                 1180                 1185             


Ser Ile  Val Ala Leu Asp Ser  Asn Lys Leu Met Asp  Phe Glu Asn 
    1190                 1195                 1200             


Leu Lys  Pro Leu Phe Leu Glu  Asn Lys Tyr Glu Lys  Ile Leu Lys 
    1205                 1210                 1215             


Asn Val  Ile Lys Phe Leu Glu  Asp Asn Gln Gly Lys  Ser Glu Glu 
    1220                 1225                 1230             


Asn Tyr  Lys Phe Ile Tyr Leu  Lys Lys Lys Asp Arg  Tyr Glu Lys 
    1235                 1240                 1245             


Asn Glu  Thr Leu Glu Ser Val  Lys Asp Arg Tyr Asn  Leu Glu Phe 
    1250                 1255                 1260             


Asn Glu  Met Tyr Asp Lys Phe  Leu Glu Lys Leu Asp  Ser Lys Asp 
    1265                 1270                 1275             


Tyr Lys  Asn Tyr Ile Asn Asn  Lys Lys Tyr Lys Asn  Leu Leu Asp 
    1280                 1285                 1290             


Val Lys  Glu Lys Phe Ile Lys  Leu Asn Leu Phe Asp  Lys Ala Phe 
    1295                 1300                 1305             


Thr Leu  Lys Ser Phe Leu Asp  Leu Phe Asn Arg Lys  Thr Met Ala 
    1310                 1315                 1320             


Asp Phe  Ser Lys Val Gly Leu  Thr Lys Tyr Leu Gly  Lys Ile Gln 
    1325                 1330                 1335             


Lys Ile  Ser Ser Asn Val Leu  Ser Lys Asn Glu Leu  Tyr Leu Leu 
    1340                 1345                 1350             


Glu Glu  Ser Val Thr Gly Leu  Phe Val Lys Lys Ile  Lys Leu 
    1355                 1360                 1365         


<210>  985
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  985

Met Lys Lys Gln Gln Phe Ser Asp Tyr Tyr Leu Gly Phe Asp Ile Gly 
1               5                   10                  15      


Thr Asn Ser Val Gly Trp Cys Val Thr Asp Phe Asn Tyr Asn Val Leu 
            20                  25                  30          


Arg Phe Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Asp Glu Ala 
        35                  40                  45              


Lys Thr Ala Ala Glu Arg Arg Val Gln Arg Asn Ser Arg Arg Arg Leu 
    50                  55                  60                  


Lys Arg Arg Lys Trp Arg Leu Asn Leu Leu Glu Glu Ile Phe Ser Asp 
65                  70                  75                  80  


Glu Ile Leu Lys Ile Asp Ser Asn Phe Phe Arg Arg Leu Lys Glu Ser 
                85                  90                  95      


Ser Leu Trp Leu Glu Asp Lys Ser Ser Lys Glu Lys Phe Thr Leu Phe 
            100                 105                 110         


Asn Asp Asp Asn Tyr Lys Asp Tyr Asp Phe Tyr Lys Gln Tyr Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Asn Glu Leu Ile Lys Asn Pro Glu Lys Lys Asp 
    130                 135                 140                 


Ile Arg Leu Val Tyr Leu Ala Leu His Ser Ile Phe Lys Ser Arg Gly 
145                 150                 155                 160 


His Phe Leu Phe Glu Gly Gln Asn Leu Lys Asp Ile Lys Asn Phe Glu 
                165                 170                 175     


Thr Leu Tyr Asn Asn Leu Met Ala Phe Leu Glu Asp Asn Asp Ile Tyr 
            180                 185                 190         


Lys Asn Ile Asp Ser Ser Tyr Ile Gly Asn Leu Glu Asn Ile Ile Cys 
        195                 200                 205             


Asp Ser Lys Lys Gly Leu Lys Asp Lys Glu Lys Glu Phe Lys Glu Ile 
    210                 215                 220                 


Phe Asn Ser Asp Lys Gln Leu Val Gly Phe Phe Lys Leu Ser Val Gly 
225                 230                 235                 240 


Ser Ser Val Ser Leu Asn Asp Leu Phe Asp Thr Asp Glu Tyr Lys Lys 
                245                 250                 255     


Gly Glu Val Glu Lys Glu Lys Ile Ser Phe Arg Glu Gln Ile Tyr Glu 
            260                 265                 270         


Asp Asp Lys Pro Ile Tyr Tyr Ser Ile Leu Gly Glu Lys Ile Glu Phe 
        275                 280                 285             


Leu Asp Ile Ala Lys Ser Phe Tyr Asp Phe Met Val Leu Asn Asn Ile 
    290                 295                 300                 


Leu Ala Asp Ser Gln Tyr Ile Ser Glu Ala Lys Val Lys Leu Tyr Asp 
305                 310                 315                 320 


Glu His Lys Arg Asp Leu Lys Asn Leu Lys Tyr Ile Ile Arg Lys Tyr 
                325                 330                 335     


Asn Lys Glu Asn Tyr Asp Lys Leu Phe Lys Asp Lys Asn Glu Ser Asn 
            340                 345                 350         


Tyr Ser Ala Tyr Ile Gly Leu Asn Lys Glu Lys Gly Lys Lys Glu Val 
        355                 360                 365             


Ile Glu Lys Ser Arg Leu Lys Ile Asp Asp Phe Ala Lys Ile Ile Lys 
    370                 375                 380                 


Gly Tyr Leu Pro Lys Ala Glu Lys Ile Asp Glu Lys Asp Arg Ser Ile 
385                 390                 395                 400 


Phe Asn Glu Ile Leu Asp Lys Ile Glu Leu Lys Thr Ile Leu Pro Lys 
                405                 410                 415     


Gln Arg Ile Ser Asp Asn Gly Thr Leu Pro Tyr Gln Ile His Glu Ala 
            420                 425                 430         


Glu Leu Glu Lys Ile Leu Glu Asn Gln Ala Lys Tyr Tyr Asp Phe Leu 
        435                 440                 445             


Asn His Glu Glu Asn Gly Ile Ser Thr Lys Asp Lys Leu Leu Met Thr 
    450                 455                 460                 


Phe Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Ser Tyr His 
465                 470                 475                 480 


Lys Asn Lys Gly Gly Asn Ser Trp Ile Val Arg Lys Glu Glu Gly Lys 
                485                 490                 495     


Ile Leu Pro Trp Asn Phe Glu Gln Lys Val Asp Ile Glu Lys Ser Ala 
            500                 505                 510         


Glu Glu Phe Ile Lys Arg Met Thr Asn Lys Cys Thr Tyr Leu Asn Gly 
        515                 520                 525             


Glu Asp Val Ile Pro Lys Asp Ser Phe Leu Tyr Ser Glu Tyr Ile Ile 
    530                 535                 540                 


Leu Asn Glu Leu Asn Lys Val Gln Val Asn Asp Glu Phe Leu Asn Lys 
545                 550                 555                 560 


Glu Ile Lys Lys Lys Ile Ile Glu Asp Leu Phe Lys Lys Ser Lys Lys 
                565                 570                 575     


Ile Ser Glu Lys Asn Phe Arg Glu Tyr Leu Leu Val Asn Gln Ile Thr 
            580                 585                 590         


Asn Lys Thr Val Glu Leu Lys Gly Val Lys Asp Ala Phe Asn Ser Asn 
        595                 600                 605             


Tyr Val Ser Tyr Ile Lys Phe Lys Asp Ile Phe Gly Asp Lys Leu Asn 
    610                 615                 620                 


Leu Asp Ile Tyr Lys Glu Ile Ser Glu Lys Ser Ile Leu Trp Lys Cys 
625                 630                 635                 640 


Leu Tyr Gly Asp Asp Lys Lys Ile Phe Glu Lys Lys Ile Lys Ser Val 
                645                 650                 655     


Tyr Gly Asp Ile Leu Thr Lys Asp Glu Ile Lys Lys Ile Asn Ser Phe 
            660                 665                 670         


Lys Phe Asn Thr Trp Gly Arg Leu Ser Glu Lys Leu Leu Thr Glu Ile 
        675                 680                 685             


Glu Phe Ile Asp Leu Glu Thr Gly Glu Cys Tyr Ser Ser Val Met Asp 
    690                 695                 700                 


Ala Leu Arg Arg Thr Asn Tyr Asn Leu Met Glu Leu Leu Ser Ser Lys 
705                 710                 715                 720 


Phe Thr Leu Gln Glu Asn Ile Asp Asn Glu Asn Lys Glu Val Ser Glu 
                725                 730                 735     


Phe Ser Tyr Arg Asp Leu Val Glu Glu Ser Tyr Val Ser Pro Ser Leu 
            740                 745                 750         


Lys Arg Ala Ile Leu Gln Thr Leu Lys Ile Tyr Glu Glu Ile Arg Lys 
        755                 760                 765             


Ile Thr Gly Arg Ile Pro Lys Lys Val Phe Ile Glu Met Ala Arg Gly 
    770                 775                 780                 


Gly Asp Glu Thr Met Lys Asn Lys Lys Ile Pro Ala Arg Gln Glu Gln 
785                 790                 795                 800 


Leu Lys Lys Leu Tyr Asp Ser Cys Gly Lys Asp Ile Ser Asn Phe Ser 
                805                 810                 815     


Ile Asp Ile Lys Glu Met Lys Asn Ser Leu Asn Ser Tyr Asp Asn Asn 
            820                 825                 830         


Ser Leu Arg Gln Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Phe Gly Lys 
        835                 840                 845             


Cys Met Tyr Thr Gly Lys Glu Ile Asp Leu Asn Arg Leu Leu Gln Asn 
    850                 855                 860                 


Asn Asp Thr Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Lys Val Ile 
865                 870                 875                 880 


Lys Asp Asp Ser Phe Asp Asn Leu Val Leu Val Leu Lys Asn Glu Asn 
                885                 890                 895     


Ala Glu Lys Ser Asn Glu Tyr Pro Leu Lys Lys Glu Ile Gln Glu Lys 
            900                 905                 910         


Met Lys Ser Phe Trp Lys Phe Leu Lys Glu Lys Asn Phe Ile Ser Asp 
        915                 920                 925             


Glu Lys Tyr Lys Arg Leu Thr Gly Lys Asp Glu Phe Glu Leu Arg Gly 
    930                 935                 940                 


Phe Met Ala Arg Gln Leu Val Asn Val Arg Gln Thr Thr Lys Glu Ala 
945                 950                 955                 960 


Gly Lys Ile Leu Gln Gln Ile Glu Pro Glu Ile Lys Ile Val Tyr Ser 
                965                 970                 975     


Lys Ala Glu Ile Ala Ser Ser Phe Arg Glu Met Phe Asp Phe Ile Lys 
            980                 985                 990         


Val Arg Glu Leu Asn Asp Thr His  His Ala Lys Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ile Val  Ala Gly Asn Val Tyr  Asn Thr Lys Phe Thr  Glu Lys Pro 
    1010                 1015                 1020             


Tyr Arg  Tyr Leu Gln Glu Ile  Lys Glu Asn Tyr Asp  Val Lys Lys 
    1025                 1030                 1035             


Ile Tyr  Asn Tyr Asp Ile Lys  Asn Ala Trp Asp Lys  Glu Lys Ser 
    1040                 1045                 1050             


Leu Glu  Ile Val Lys Lys Asn  Met Glu Lys Asn Thr  Val Asn Ile 
    1055                 1060                 1065             


Thr Arg  Phe Ile Lys Glu Glu  Lys Gly Gln Leu Phe  Asp Leu Asn 
    1070                 1075                 1080             


Pro Ile  Arg Lys Gly Glu Thr  Ser Asn Glu Ile Ile  Ala Ile Lys 
    1085                 1090                 1095             


Pro Lys  Phe Tyr Glu Gly Ser  Thr Glu Lys Leu Asn  Glu Lys Tyr 
    1100                 1105                 1110             


Gly Tyr  Tyr Lys Ser Leu Asn  Pro Ala Tyr Phe Ile  Tyr Val Glu 
    1115                 1120                 1125             


His Lys  Glu Lys Asn Lys Ile  Ile Arg Ser Phe Glu  Arg Val Asn 
    1130                 1135                 1140             


Leu Val  Asp Val Asn Lys Ile  Lys Asp Glu Lys Ser  Leu Ile Lys 
    1145                 1150                 1155             


Tyr Leu  Ile Glu Asn Lys Gly  Leu Ile Glu Pro Lys  Val Ile Lys 
    1160                 1165                 1170             


Lys Val  Tyr Lys Arg Gln Val  Ile Leu Ile Asn Asn  Phe Pro Tyr 
    1175                 1180                 1185             


Ser Ile  Val Ala Leu Asp Ser  Asn Lys Leu Met Asp  Phe Glu Asn 
    1190                 1195                 1200             


Leu Lys  Pro Leu Phe Leu Glu  Arg Lys Tyr Glu Lys  Ile Leu Lys 
    1205                 1210                 1215             


Asn Ala  Ile Lys Phe Leu Glu  Asp Asn Gln Gly Lys  Thr Glu Glu 
    1220                 1225                 1230             


Tyr Tyr  Lys Phe Leu Tyr Leu  Lys Lys Lys Asp Arg  Asn Glu Lys 
    1235                 1240                 1245             


Asn Glu  Thr Ile Asp Ser Val  Lys Glu Arg Tyr Asn  Ile Glu Phe 
    1250                 1255                 1260             


Asn Glu  Met Tyr Asp Lys Phe  Leu Glu Lys Leu Asp  Ser Lys Asp 
    1265                 1270                 1275             


Tyr Lys  Asn Tyr Ile Asn Asn  Lys Lys Tyr Ser Glu  Leu Val Asn 
    1280                 1285                 1290             


Val Lys  Glu Lys Phe Ile Lys  Leu Asn Leu Phe Asp  Lys Ala Phe 
    1295                 1300                 1305             


Thr Leu  Lys Ser Phe Leu Asp  Leu Phe Asn Arg Lys  Thr Met Ala 
    1310                 1315                 1320             


Asp Phe  Ser Lys Val Gly Leu  Thr Lys Tyr Leu Gly  Lys Ile Gln 
    1325                 1330                 1335             


Lys Ile  Ser Ser Asn Val Leu  Ser Lys Asn Glu Leu  His Leu Leu 
    1340                 1345                 1350             


Glu Glu  Ser Val Thr Gly Leu  Phe Val Lys Lys Ile  Lys Leu 
    1355                 1360                 1365         


<210>  986
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  986

Met Lys Asp Tyr Tyr Leu Gly Leu Asp Met Gly Ser Ser Ser Val Gly 
1               5                   10                  15      


Trp Ala Leu Ser Asp Glu Arg Tyr Asn Leu Leu Arg Ala Lys Gly Lys 
            20                  25                  30          


Asp Leu Trp Gly Val Arg Leu Phe Asp Glu Ala Lys Thr Ala Ala Glu 
        35                  40                  45              


Arg Arg Thr Phe Arg Ser Gln Arg Arg Arg Leu Glu Arg Ser Lys Trp 
    50                  55                  60                  


Arg Leu Ser Leu Leu Thr Ser Leu Phe Glu Glu Glu Ile Ser Lys Ile 
65                  70                  75                  80  


Asp Asp Lys Phe Phe Asp Arg Leu Lys Asp Ser Lys Phe Phe Ile Asp 
                85                  90                  95      


Asp Lys Ser Asp Gln Gln Pro Asn Thr Leu Phe Asn Asp Val Asn Phe 
            100                 105                 110         


Thr Asp Lys Glu Tyr His Lys Arg Tyr Pro Ser Ile Tyr His Leu Arg 
        115                 120                 125             


Asn Ala Leu Phe Asn Gln Asn Asp Lys Phe Asp Ile Arg Leu Ile Phe 
    130                 135                 140                 


Leu Ala Leu Ser His Ile Val Lys Lys Arg Gly His Phe Leu Phe Asp 
145                 150                 155                 160 


Gly Asp Lys Ala Gly Phe Gly Glu Asp Ile Asp Val Ile Leu Asp Glu 
                165                 170                 175     


Leu Lys Ala Tyr Leu Gln Asp Ala Phe His Thr Lys Leu Glu Phe Thr 
            180                 185                 190         


Ser Glu Phe Lys Ser Ile Met Leu Asp Lys Asn Leu Arg Lys Lys Gln 
        195                 200                 205             


Lys Glu Glu Glu Leu Lys Asn Ser Ile Lys Ile Ser Ser Asp Thr Ile 
    210                 215                 220                 


Asn Ser Lys Asp Gly Lys Ser Ile Leu Ile Lys Leu Phe Leu Gly Tyr 
225                 230                 235                 240 


Glu Ala Asn Leu Lys Pro Phe Phe Leu Glu Tyr Lys Val Glu Lys Asp 
                245                 250                 255     


Asn Glu Tyr Lys Ile Asn Phe Ser Lys Glu Asp Tyr Glu Glu Lys Leu 
            260                 265                 270         


Leu Asp Met Thr Ser Lys Phe Glu Glu Thr Thr Tyr Phe Ile Glu Ile 
        275                 280                 285             


Ala Lys Lys Ile His Asp Tyr Phe Leu Leu Glu Glu Ile Met Leu Gly 
    290                 295                 300                 


Phe Lys Asn Ile Ser Ala Ser Lys Val Ser Ile Tyr Gln Lys His Lys 
305                 310                 315                 320 


Asn Asp Leu Lys Thr Leu Lys Asn Ile Val Lys Asn Gln Ile Asn Asn 
                325                 330                 335     


Ser Tyr Asn Ala Ile Phe Lys Asp Ile Gly Leu Asp Asn Tyr Pro Ala 
            340                 345                 350         


Tyr Val Ser Cys Trp Asn Ser Lys Lys His Lys Glu Lys Gln Lys Gly 
        355                 360                 365             


Cys Thr Lys Asp Lys Phe Tyr Ala Phe Leu Lys Thr Lys Leu Lys Asn 
    370                 375                 380                 


Ile Asn Asn Ala Lys Glu Ile Leu Asp Ser Ile Asp Asn Asp Asp Phe 
385                 390                 395                 400 


Leu Pro Arg Leu Val Asp Lys Asn Ser Ile Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Ala Glu Leu Ile Lys Ile Ile Asp Asn Gln Ser Gln Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Ile Lys Asp Lys Ile Ile Ser Ile Phe Lys Phe Arg 
        435                 440                 445             


Ile Pro Phe Tyr Val Gly Pro Leu Asn Asn Phe His Lys Asp Lys Gly 
    450                 455                 460                 


Gly Asn Ala Trp Ile Glu Lys Leu Lys Asp Glu Arg Ile Phe Pro Trp 
465                 470                 475                 480 


Asn Phe Asn Asp Val Val Asp Leu Asn Lys Ser Ala Ala Asn Phe Ile 
                485                 490                 495     


Glu Lys Met Ile Asn Glu Cys Ser Tyr Leu Lys Gly Glu Lys Val Leu 
            500                 505                 510         


Ala Lys Asn Ser Leu Leu Tyr Thr Lys Tyr Ser Val Leu Asn Leu Ile 
        515                 520                 525             


Asn Lys Leu Lys Ile Asn Gly Thr Ala Val Ser Val Glu Thr Lys Gln 
    530                 535                 540                 


Lys Ile Tyr Asn Glu Leu Leu Leu Lys Gln Lys Asn Ile Lys Lys Thr 
545                 550                 555                 560 


Ala Ile Lys Lys Phe Leu Ile Asn Asn Ser Ile Ile Asp Lys Asn Asp 
                565                 570                 575     


Glu Ile Ser Gly Ile Asp Asp Ser Leu Thr Ser Asn Leu Ala Val Phe 
            580                 585                 590         


Ile Asp Phe Glu Lys Ile Ser Asn Arg Leu Arg Tyr Asp Gln Lys Glu 
        595                 600                 605             


Asp Ile Ile Lys Ser Ile Ser Leu Phe Gly Tyr Asp Asn Ala Met Leu 
    610                 615                 620                 


Lys Lys Gln Ile Ile Thr Ile Ala Pro Asp Leu Ser Glu Asp Glu Leu 
625                 630                 635                 640 


Glu Lys Leu Ser Lys Leu Arg Tyr Ser Gly Phe Gly Arg Leu Ser Lys 
                645                 650                 655     


Arg Leu Leu Thr Ser Ile Lys His Asn Gly Lys Ser Ile Ile Glu Met 
            660                 665                 670         


Leu Trp Asp Thr Asn Asp Asn Leu Met Glu Leu Leu Ser Asp Arg Tyr 
        675                 680                 685             


Thr Phe Ile Glu Gln Ile Asn Lys His Asn Asp Gly Phe Lys Glu Asp 
    690                 695                 700                 


Ile Asn Leu Asn Phe Gln Asn Val Asp Thr Leu Tyr Val Ser Pro Ser 
705                 710                 715                 720 


Val Lys Arg Gln Ile Trp Gln Ala Phe Lys Val Ile Asn Glu Leu Ile 
                725                 730                 735     


Ser Val Met Gly Arg Glu Pro Lys Lys Ile Phe Val Glu Met Ala Arg 
            740                 745                 750         


Glu Glu Gly Glu Lys Lys Arg Thr Gln Ala Arg Lys Asp Lys Val Leu 
        755                 760                 765             


Glu Leu Tyr Lys Ser Ile Lys Asn Asn Glu Ile Ser Thr Phe Leu Thr 
    770                 775                 780                 


Asn Phe Lys Asp Ile Lys Thr Lys Leu Glu Gly Glu Ser Asn Leu Asn 
785                 790                 795                 800 


Ser Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Leu Gly Val Asp Ile Tyr 
                805                 810                 815     


Thr Gly Glu Lys Ile Pro Phe Glu Lys Leu Phe Asp Gln Asn Ile Tyr 
            820                 825                 830         


Asp Ile Asp His Val Ile Pro Arg Ser Leu Lys Lys Asp Asp Ser Phe 
        835                 840                 845             


Asp Asn Leu Val Leu Thr Ser Lys Thr Asn Asn Ala Lys Lys Thr Asp 
    850                 855                 860                 


Glu Phe Pro Val Ser Ser Glu Ile Gln Ser Lys Met Gln Thr Phe Trp 
865                 870                 875                 880 


Lys Val Leu Lys Asp Lys Asp Leu Met Ser Glu Ile Lys Tyr Thr Asn 
                885                 890                 895     


Leu Thr Arg Lys Thr His Leu Ser Asp Asp Glu Leu Gly Asp Phe Ile 
            900                 905                 910         


Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala Leu Thr Gln 
        915                 920                 925             


Ile Leu Lys Asn Val Tyr Ala Asn Ser Asp Ile Val Tyr Val Lys Ala 
    930                 935                 940                 


Gly Ile Val Ser Glu Phe Arg Gln Gln Phe Asp Met Leu Lys Ile Arg 
945                 950                 955                 960 


Glu Leu Asn Asp Phe His His Ala Asn Asp Ala Tyr Leu Asn Ile Val 
                965                 970                 975     


Ile Gly Asn Val Tyr Asn Ile Lys Phe Thr Lys Asn Pro Arg Asn Tyr 
            980                 985                 990         


Val Lys Glu Tyr Tyr Lys Lys Gly  Glu His Tyr Asn Leu  Lys Ile Glu 
        995                 1000                 1005             


Lys Met  Leu Glu Lys Asp Ile  Ala Arg Gly Gly Asn  Phe Gly Trp 
    1010                 1015                 1020             


Asp Lys  Lys His Ser Ile Glu  Thr Val Gln Lys Thr  Met Lys Lys 
    1025                 1030                 1035             


Gln Ser  Pro Leu Ile Thr Tyr  Arg Thr Phe Glu Asn  Lys Gly Glu 
    1040                 1045                 1050             


Phe Phe  Asn Gln Gln Pro Ser  Lys Ala Ser Ala Asn  Leu Leu Ala 
    1055                 1060                 1065             


Leu Lys  Ser Ser Asp Lys Arg  Leu Leu Asp Thr Val  Arg Tyr Gly 
    1070                 1075                 1080             


Gly Tyr  Lys Gly Thr Gln Tyr  Ala His Met Phe Leu  Val Glu His 
    1085                 1090                 1095             


Phe Asn  Gly Lys Ile Ile Lys  Glu Ile Met Gly Val  Pro Ile Tyr 
    1100                 1105                 1110             


Leu Ser  Lys Glu Ile Lys Asp  Asp Lys Ala Lys Leu  Leu Glu Tyr 
    1115                 1120                 1125             


Cys Asn  Asp Val Leu Lys Leu  Lys Asn Pro Lys Ile  Leu Phe Ser 
    1130                 1135                 1140             


Lys Ile  Pro Tyr Asn Ser Leu  Ile Val Tyr Asn Gly  Tyr Pro Ile 
    1145                 1150                 1155             


Leu Leu  Arg Gly Leu Thr Gly  Val Arg Gly Ala Ala  Gln Leu Lys 
    1160                 1165                 1170             


Leu Asp  Gln Thr Ser Thr Gln  Val Val Lys Asn Ile  Val Lys Leu 
    1175                 1180                 1185             


Tyr Asp  Trp Leu Ser Gln Lys  Gln Lys Glu Leu Ser  Asp Leu Ser 
    1190                 1195                 1200             


Lys Lys  Ile Glu Phe Phe Gln  Glu Asn Asn Lys Lys  Asp Glu Val 
    1205                 1210                 1215             


Lys Ala  Ala Met Leu Glu Ala  Asp Asn Ile Leu Phe  Ile Leu Asn 
    1220                 1225                 1230             


Asp Arg  Phe Lys Asn Thr Lys  Ile Gly Lys Asp Ile  Met Asn Asp 
    1235                 1240                 1245             


Asn His  Leu Asn Asn Leu Tyr  Asp Thr Phe Ile Asn  Lys Leu Glu 
    1250                 1255                 1260             


Asn Ser  Ile Tyr Lys Asn Lys  Pro Gly Gly Lys Gln  Asp Val Leu 
    1265                 1270                 1275             


Met Lys  Glu Lys Arg Asp Leu  Phe Leu Ser Phe Ser  Met Ser Lys 
    1280                 1285                 1290             


Lys Cys  Ile Thr Leu Tyr Lys  Ile Ile Lys Ile Phe  Gly Ser Cys 
    1295                 1300                 1305             


Asp Gln  Gly Val Asp Phe Ser  Asp Ile Ala Glu Lys  Ile Ser Lys 
    1310                 1315                 1320             


Ser Gly  Gly Ile Gly Gly Lys  Asn Leu Gly Lys Asn  Thr Phe Asp 
    1325                 1330                 1335             


Leu Val  Phe Lys Glu Asn Ser  Thr Tyr Thr Leu Val  Gln Ser Ser 
    1340                 1345                 1350             


Val Thr  Gly Leu Tyr Ala Lys  Lys Thr Asp Leu His  Lys Leu 
    1355                 1360                 1365         


<210>  987
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  987

Met Lys Lys Gln Lys Phe Ser Asp Tyr Tyr Leu Gly Phe Asp Ile Gly 
1               5                   10                  15      


Thr Asn Ser Val Gly Trp Cys Val Thr Asp Leu Asp Tyr Asn Val Leu 
            20                  25                  30          


Arg Phe Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Glu Glu Ala 
        35                  40                  45              


Lys Thr Ala Ala Glu Arg Arg Val Gln Arg Asn Ser Arg Arg Arg Leu 
    50                  55                  60                  


Lys Arg Arg Lys Trp Arg Leu Asn Leu Leu Glu Glu Ile Phe Ser Asn 
65                  70                  75                  80  


Glu Ile Leu Lys Ile Asp Ser Asn Phe Phe Arg Arg Leu Lys Glu Ser 
                85                  90                  95      


Ser Leu Trp Leu Glu Asp Lys Ser Ser Lys Glu Lys Phe Thr Leu Phe 
            100                 105                 110         


Asn Asp Asp Asn Tyr Lys Asp Tyr Asp Phe Tyr Lys Gln Tyr Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Asn Glu Leu Ile Lys Asn Pro Glu Lys Lys Asp 
    130                 135                 140                 


Ile Arg Leu Val Tyr Leu Ala Ile His Ser Ile Phe Lys Ser Arg Gly 
145                 150                 155                 160 


His Phe Leu Phe Glu Gly Gln Asn Leu Lys Glu Ile Lys Asn Phe Glu 
                165                 170                 175     


Thr Leu Tyr Asn Asn Leu Ile Ala Phe Leu Glu Asp Asn Gly Ile Asn 
            180                 185                 190         


Lys Ile Ile Asp Lys Asn Asn Ile Glu Lys Leu Glu Lys Ile Val Cys 
        195                 200                 205             


Asp Ser Lys Lys Gly Leu Lys Asp Lys Glu Lys Glu Phe Lys Glu Ile 
    210                 215                 220                 


Phe Asn Ser Asp Lys Gln Leu Val Ala Ile Phe Lys Leu Ser Val Gly 
225                 230                 235                 240 


Ser Ser Val Ser Leu Asn Asp Leu Phe Asp Thr Asp Glu Tyr Lys Lys 
                245                 250                 255     


Gly Glu Val Glu Lys Glu Lys Ile Ser Phe Arg Glu Gln Ile Tyr Glu 
            260                 265                 270         


Asp Asp Lys Pro Ile Tyr Tyr Ser Ile Leu Gly Glu Lys Ile Glu Leu 
        275                 280                 285             


Leu Asp Ile Ala Lys Thr Phe Tyr Asp Phe Met Val Leu Asn Asn Ile 
    290                 295                 300                 


Leu Ala Asp Ser Gln Tyr Ile Ser Glu Ala Lys Val Lys Leu Tyr Glu 
305                 310                 315                 320 


Glu His Lys Lys Asp Leu Lys Asn Leu Lys Tyr Ile Ile Arg Lys Tyr 
                325                 330                 335     


Asn Lys Gly Asn Tyr Asp Lys Leu Phe Lys Asp Lys Asn Glu Asn Asn 
            340                 345                 350         


Tyr Ser Ala Tyr Ile Gly Leu Asn Lys Glu Lys Ser Lys Lys Glu Val 
        355                 360                 365             


Ile Glu Lys Ser Arg Leu Lys Ile Asp Asp Leu Ile Lys Asn Ile Lys 
    370                 375                 380                 


Gly Tyr Leu Pro Lys Val Glu Glu Ile Glu Glu Lys Asp Lys Ala Ile 
385                 390                 395                 400 


Phe Asn Lys Ile Leu Asn Lys Ile Glu Leu Lys Thr Ile Leu Pro Lys 
                405                 410                 415     


Gln Arg Ile Ser Asp Asn Gly Thr Leu Pro Tyr Gln Ile His Glu Ala 
            420                 425                 430         


Glu Leu Glu Lys Ile Leu Glu Asn Gln Ser Lys Tyr Tyr Asp Phe Leu 
        435                 440                 445             


Asn Tyr Glu Glu Asn Gly Ile Ile Thr Lys Asp Lys Leu Leu Met Thr 
    450                 455                 460                 


Phe Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Ser Tyr His 
465                 470                 475                 480 


Lys Asp Lys Gly Gly Asn Ser Trp Ile Val Arg Lys Glu Glu Gly Lys 
                485                 490                 495     


Ile Leu Pro Trp Asn Phe Glu Gln Lys Val Asp Ile Glu Lys Ser Ala 
            500                 505                 510         


Glu Glu Phe Ile Lys Arg Met Thr Asn Lys Cys Thr Tyr Leu Asn Gly 
        515                 520                 525             


Glu Asp Val Ile Pro Lys Asp Thr Phe Leu Tyr Ser Glu Tyr Val Ile 
    530                 535                 540                 


Leu Asn Glu Leu Asn Lys Val Gln Val Asn Asp Glu Phe Leu Asn Glu 
545                 550                 555                 560 


Glu Asn Lys Arg Lys Ile Ile Asp Glu Leu Phe Lys Glu Asn Lys Lys 
                565                 570                 575     


Val Ser Glu Lys Lys Phe Lys Glu Tyr Leu Leu Val Lys Gln Ile Val 
            580                 585                 590         


Asp Gly Thr Ile Glu Leu Lys Gly Val Lys Asp Ser Phe Asn Ser Asn 
        595                 600                 605             


Tyr Ile Ser Tyr Ile Arg Phe Lys Asp Ile Phe Gly Glu Lys Leu Asn 
    610                 615                 620                 


Leu Asp Ile Tyr Lys Glu Ile Ser Glu Lys Ser Ile Leu Trp Lys Cys 
625                 630                 635                 640 


Leu Tyr Gly Asp Asp Lys Lys Ile Phe Glu Lys Lys Ile Lys Asn Glu 
                645                 650                 655     


Tyr Gly Asp Ile Leu Thr Lys Asp Glu Ile Lys Lys Ile Asn Thr Phe 
            660                 665                 670         


Lys Phe Asn Asn Trp Gly Arg Leu Ser Glu Lys Leu Leu Thr Gly Ile 
        675                 680                 685             


Glu Phe Ile Asn Leu Glu Thr Gly Glu Cys Tyr Ser Ser Val Met Asp 
    690                 695                 700                 


Ala Leu Arg Arg Thr Asn Tyr Asn Leu Met Glu Leu Leu Ser Ser Lys 
705                 710                 715                 720 


Phe Thr Leu Gln Glu Ser Ile Asn Asn Glu Asn Lys Glu Met Asn Glu 
                725                 730                 735     


Ala Ser Tyr Arg Asp Leu Ile Glu Glu Ser Tyr Val Ser Pro Ser Leu 
            740                 745                 750         


Lys Arg Ala Ile Phe Gln Thr Leu Lys Ile Tyr Glu Glu Ile Arg Lys 
        755                 760                 765             


Ile Thr Gly Arg Val Pro Lys Lys Val Phe Ile Glu Met Ala Arg Gly 
    770                 775                 780                 


Gly Asp Glu Ser Met Lys Asn Lys Lys Ile Pro Ala Arg Gln Glu Gln 
785                 790                 795                 800 


Leu Lys Lys Leu Tyr Asp Ser Cys Gly Asn Asp Ile Ala Asn Phe Ser 
                805                 810                 815     


Ile Asp Ile Lys Glu Met Lys Asn Ser Leu Ile Ser Tyr Asp Asn Asn 
            820                 825                 830         


Ser Leu Arg Gln Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Phe Gly Lys 
        835                 840                 845             


Cys Met Tyr Thr Gly Arg Glu Ile Asp Leu Asp Arg Leu Leu Gln Asn 
    850                 855                 860                 


Asn Asp Thr Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Lys Val Ile 
865                 870                 875                 880 


Lys Asp Asp Ser Phe Asp Asn Leu Val Leu Val Leu Lys Asn Glu Asn 
                885                 890                 895     


Ala Glu Lys Ser Asn Glu Tyr Pro Val Lys Lys Glu Ile Gln Glu Lys 
            900                 905                 910         


Met Lys Ser Phe Trp Arg Phe Leu Lys Glu Lys Asn Phe Ile Ser Asp 
        915                 920                 925             


Glu Lys Tyr Lys Arg Leu Thr Gly Lys Asp Asp Phe Glu Leu Arg Gly 
    930                 935                 940                 


Phe Met Ala Arg Gln Leu Val Asn Val Arg Gln Thr Thr Lys Glu Val 
945                 950                 955                 960 


Gly Lys Ile Leu Gln Gln Ile Glu Pro Glu Ile Lys Ile Val Tyr Ser 
                965                 970                 975     


Lys Ala Glu Ile Ala Ser Ser Phe Arg Glu Met Phe Asp Phe Ile Lys 
            980                 985                 990         


Val Arg Glu Leu Asn Asp Thr His  His Ala Lys Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ile Val  Ala Gly Asn Val Tyr  Asn Thr Lys Phe Thr  Glu Lys Pro 
    1010                 1015                 1020             


Tyr Arg  Tyr Leu Gln Glu Ile  Lys Glu Asn Tyr Asp  Val Lys Lys 
    1025                 1030                 1035             


Ile Tyr  Asn Tyr Asp Ile Lys  Asn Ala Trp Asp Lys  Glu Asn Ser 
    1040                 1045                 1050             


Leu Glu  Ile Val Lys Lys Asn  Met Glu Lys Asn Thr  Val Asn Ile 
    1055                 1060                 1065             


Thr Arg  Phe Ile Lys Glu Lys  Lys Gly Gln Leu Phe  Asp Leu Asn 
    1070                 1075                 1080             


Pro Ile  Lys Lys Gly Glu Thr  Ser Asn Glu Ile Ile  Ser Ile Lys 
    1085                 1090                 1095             


Pro Lys  Val Tyr Asn Gly Lys  Asp Asp Lys Leu Asn  Glu Lys Tyr 
    1100                 1105                 1110             


Gly Tyr  Tyr Lys Ser Leu Asn  Pro Ala Tyr Phe Leu  Tyr Val Glu 
    1115                 1120                 1125             


His Lys  Glu Lys Asn Lys Arg  Ile Lys Ser Phe Glu  Arg Val Asn 
    1130                 1135                 1140             


Leu Val  Asp Val Asn Asn Ile  Lys Asp Glu Lys Ser  Leu Val Lys 
    1145                 1150                 1155             


Tyr Leu  Ile Glu Asn Lys Lys  Leu Val Glu Pro Arg  Val Ile Lys 
    1160                 1165                 1170             


Lys Val  Tyr Lys Arg Gln Val  Ile Leu Ile Asn Asp  Tyr Pro Tyr 
    1175                 1180                 1185             


Ser Ile  Val Thr Leu Asp Ser  Asn Lys Leu Met Asp  Phe Glu Asn 
    1190                 1195                 1200             


Leu Lys  Pro Leu Phe Leu Glu  Asn Lys Tyr Glu Lys  Ile Leu Lys 
    1205                 1210                 1215             


Asn Val  Ile Lys Phe Leu Glu  Asp Asn Gln Gly Lys  Ser Glu Glu 
    1220                 1225                 1230             


Asn Tyr  Lys Phe Ile Tyr Leu  Lys Lys Lys Asp Arg  Tyr Glu Lys 
    1235                 1240                 1245             


Asn Glu  Thr Leu Glu Ser Val  Lys Asp Arg Tyr Asn  Leu Glu Phe 
    1250                 1255                 1260             


Asn Glu  Met Tyr Asp Lys Phe  Leu Glu Lys Leu Asp  Ser Lys Asp 
    1265                 1270                 1275             


Tyr Lys  Asn Tyr Met Asn Asn  Lys Lys Tyr Gln Glu  Leu Leu Asp 
    1280                 1285                 1290             


Val Lys  Glu Lys Phe Ile Lys  Leu Asn Leu Phe Asp  Lys Ala Phe 
    1295                 1300                 1305             


Thr Leu  Lys Ser Phe Leu Asp  Leu Phe Asn Arg Lys  Thr Met Ala 
    1310                 1315                 1320             


Asp Phe  Ser Lys Val Gly Leu  Thr Lys Tyr Leu Gly  Lys Ile Gln 
    1325                 1330                 1335             


Lys Ile  Ser Ser Asn Val Leu  Ser Lys Asn Glu Leu  Tyr Leu Leu 
    1340                 1345                 1350             


Glu Glu  Ser Val Thr Gly Leu  Phe Val Lys Lys Ile  Lys Leu 
    1355                 1360                 1365         


<210>  988
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  988

Met Lys Lys Gln Lys Phe Ser Asp Tyr Tyr Leu Gly Phe Asp Ile Gly 
1               5                   10                  15      


Thr Asn Ser Val Gly Trp Cys Val Thr Asp Met Asn Tyr Asn Val Leu 
            20                  25                  30          


Arg Phe Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Glu Glu Ala 
        35                  40                  45              


Lys Thr Ala Ala Glu Arg Arg Val Gln Arg Asn Ser Arg Arg Arg Leu 
    50                  55                  60                  


Lys Arg Arg Lys Trp Arg Leu Asn Leu Leu Glu Glu Ile Phe Ser Asn 
65                  70                  75                  80  


Glu Ile Leu Lys Ile Asp Ser Asn Phe Phe Arg Arg Leu Lys Glu Ser 
                85                  90                  95      


Ser Leu Trp Leu Glu Asp Lys Ser Ser Lys Glu Lys Phe Thr Leu Phe 
            100                 105                 110         


Asn Asp Asp Asn Tyr Lys Asp Tyr Asp Phe Tyr Lys Gln Tyr Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Asn Glu Leu Ile Lys Asn Pro Glu Lys Lys Asp 
    130                 135                 140                 


Ile Arg Leu Val Tyr Leu Ala Ile His Ser Ile Phe Lys Ser Arg Gly 
145                 150                 155                 160 


His Phe Leu Phe Glu Gly Gln Asn Leu Lys Glu Ile Lys Asn Phe Glu 
                165                 170                 175     


Thr Leu Tyr Asn Asn Leu Ile Ala Phe Leu Glu Asp Asn Gly Ile Asn 
            180                 185                 190         


Lys Ser Ile Asp Lys Asp Asn Leu Glu Lys Leu Glu Lys Ile Val Cys 
        195                 200                 205             


Asp Ser Gly Lys Gly Leu Lys Asp Lys Glu Lys Glu Phe Lys Glu Ile 
    210                 215                 220                 


Phe Asn Ser Asp Lys Gln Leu Val Ala Ile Phe Lys Leu Ser Val Gly 
225                 230                 235                 240 


Leu Ser Val Ser Leu Asn Asp Leu Phe Asp Thr Asp Glu Tyr Lys Lys 
                245                 250                 255     


Gly Glu Val Glu Lys Glu Lys Ile Ser Phe Arg Glu Gln Ile Tyr Glu 
            260                 265                 270         


Asp Asp Lys Pro Ile Tyr Tyr Ser Ile Leu Gly Glu Lys Ile Glu Leu 
        275                 280                 285             


Leu Asp Ile Ala Lys Ser Phe Tyr Asp Phe Met Val Leu Asn Asn Ile 
    290                 295                 300                 


Leu Ala Asp Ser Gln Tyr Ile Ser Glu Ala Lys Val Lys Leu Tyr Glu 
305                 310                 315                 320 


Glu His Lys Arg Asp Leu Lys Asn Leu Lys Tyr Ile Ile Arg Lys Tyr 
                325                 330                 335     


Asn Arg Glu Asn Tyr Asp Lys Leu Phe Lys Asp Lys Asn Glu Ser Asn 
            340                 345                 350         


Tyr Pro Ala Tyr Ile Gly Leu Asn Lys Glu Lys Ser Lys Lys Glu Val 
        355                 360                 365             


Ile Glu Lys Ser Lys Leu Lys Ile Asp Asp Leu Ile Lys Ser Ile Lys 
    370                 375                 380                 


Gly Tyr Leu Pro Lys Val Glu Glu Ile Glu Glu Lys Asp Lys Ala Ile 
385                 390                 395                 400 


Phe Asn Glu Ile Leu Asn Lys Ile Glu Leu Lys Ile Ile Leu Pro Lys 
                405                 410                 415     


Gln Arg Ile Ser Asp Asn Gly Thr Leu Pro Tyr Gln Ile His Glu Ala 
            420                 425                 430         


Glu Leu Glu Lys Ile Leu Glu Asn Gln Ser Lys Tyr Tyr Asp Phe Leu 
        435                 440                 445             


Asn Tyr Glu Glu Asn Gly Ile Ile Thr Lys Asp Lys Leu Leu Met Thr 
    450                 455                 460                 


Phe Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Ser Tyr His 
465                 470                 475                 480 


Lys Asp Lys Gly Gly Asn Ser Trp Ile Val Arg Lys Glu Glu Gly Lys 
                485                 490                 495     


Ile Leu Pro Trp Asn Phe Glu Gln Lys Val Asp Ile Glu Lys Ser Ala 
            500                 505                 510         


Glu Glu Phe Ile Lys Arg Met Thr Asn Lys Cys Thr Tyr Leu Asn Gly 
        515                 520                 525             


Glu Asp Val Ile Pro Lys Asp Ser Phe Leu Tyr Ser Glu Tyr Ile Ile 
    530                 535                 540                 


Leu Asn Glu Leu Asn Lys Val Gln Val Asn Asp Glu Phe Leu Asn Glu 
545                 550                 555                 560 


Glu Asn Lys Arg Lys Ile Ile Glu Glu Leu Phe Lys Gly Asn Lys Lys 
                565                 570                 575     


Val Ser Glu Lys Lys Phe Lys Glu Tyr Leu Leu Val Asn Gln Ile Thr 
            580                 585                 590         


Thr Gly Thr Ile Glu Leu Lys Gly Ile Lys Asp Ser Phe Asn Ser Asn 
        595                 600                 605             


Tyr Ile Ser Tyr Ile Lys Phe Lys Asp Ile Phe Gly Glu Lys Leu Asn 
    610                 615                 620                 


Leu Asp Ile Tyr Lys Glu Ile Ser Glu Lys Ser Ile Leu Trp Lys Cys 
625                 630                 635                 640 


Leu Tyr Gly Asp Asp Lys Lys Ile Phe Glu Lys Lys Ile Lys Asn Glu 
                645                 650                 655     


Tyr Gly Asp Ile Leu Thr Lys Asp Glu Ile Lys Lys Ile Asn Thr Phe 
            660                 665                 670         


Lys Phe Asn Asn Trp Gly Arg Leu Ser Glu Lys Leu Leu Thr Gly Ile 
        675                 680                 685             


Glu Phe Ile Asn Leu Glu Thr Gly Glu Cys Tyr Ser Ser Val Met Glu 
    690                 695                 700                 


Ala Leu Arg Arg Thr Asn Tyr Asn Leu Met Glu Leu Leu Ser Ser Lys 
705                 710                 715                 720 


Phe Thr Leu Gln Glu Asn Ile Asp Asn Glu Asn Lys Asp Ile Ser Gln 
                725                 730                 735     


Phe Ser Tyr Arg Asp Leu Val Glu Glu Ser Tyr Val Ser Pro Ser Leu 
            740                 745                 750         


Lys Arg Ala Ile Leu Gln Thr Leu Lys Ile Tyr Glu Glu Ile Lys Lys 
        755                 760                 765             


Ile Thr Gly Arg Val Pro Lys Lys Val Phe Ile Glu Met Ala Arg Gly 
    770                 775                 780                 


Gly Asp Glu Ser Met Lys Asn Lys Lys Ile Pro Ala Arg Gln Glu Gln 
785                 790                 795                 800 


Leu Lys Lys Leu Tyr Asp Ser Cys Gly Asn Asp Ile Ala Asn Phe Ser 
                805                 810                 815     


Ile Asp Ile Lys Glu Met Lys Ser Ser Leu Ser Leu Tyr Asp Asn Asn 
            820                 825                 830         


Ser Leu Arg Gln Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Phe Gly Lys 
        835                 840                 845             


Cys Met Tyr Thr Gly Arg Glu Ile Asp Leu Asp Arg Leu Leu Gln Asn 
    850                 855                 860                 


Asn Asp Thr Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Lys Val Ile 
865                 870                 875                 880 


Lys Asp Asp Ser Phe Asp Asn Leu Val Leu Val Leu Lys Asn Glu Asn 
                885                 890                 895     


Ala Glu Lys Ser Asn Glu Tyr Pro Val Lys Lys Glu Ile Gln Glu Lys 
            900                 905                 910         


Met Lys Ser Phe Trp Arg Phe Leu Lys Glu Lys Asn Phe Ile Ser Asp 
        915                 920                 925             


Glu Lys Tyr Lys Arg Leu Thr Gly Lys Asp Asp Phe Glu Leu Arg Gly 
    930                 935                 940                 


Phe Met Ala Arg Gln Leu Val Asn Val Arg Gln Thr Thr Lys Glu Val 
945                 950                 955                 960 


Gly Lys Ile Leu Gln Gln Ile Glu Pro Glu Ile Lys Ile Val Tyr Ser 
                965                 970                 975     


Lys Ala Glu Ile Ala Ser Ser Phe Arg Glu Met Phe Asp Phe Ile Lys 
            980                 985                 990         


Val Arg Glu Leu Asn Asp Thr His  His Ala Lys Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ile Val  Ala Gly Asn Val Tyr  Asn Thr Lys Phe Thr  Glu Lys Pro 
    1010                 1015                 1020             


Tyr Arg  Tyr Leu Gln Glu Ile  Lys Glu Asn Tyr Asp  Val Lys Lys 
    1025                 1030                 1035             


Ile Tyr  Ser Tyr Asp Ile Lys  Asn Ala Trp Asp Lys  Glu Asn Ser 
    1040                 1045                 1050             


Leu Glu  Ile Val Lys Lys Asn  Met Glu Lys Asn Thr  Val Asn Ile 
    1055                 1060                 1065             


Thr Arg  Phe Ile Lys Glu Glu  Lys Gly Gln Leu Phe  Asp Leu Asn 
    1070                 1075                 1080             


Pro Ile  Lys Lys Gly Glu Thr  Ser Asn Glu Ile Ile  Ser Ile Lys 
    1085                 1090                 1095             


Pro Lys  Val Tyr Asn Gly Lys  Asp Asp Lys Leu Asn  Glu Lys Tyr 
    1100                 1105                 1110             


Gly Tyr  Tyr Lys Ser Leu Asn  Pro Ala Tyr Phe Leu  Tyr Val Glu 
    1115                 1120                 1125             


His Lys  Glu Lys Asn Lys Arg  Ile Lys Ser Phe Glu  Arg Val Asn 
    1130                 1135                 1140             


Leu Val  Asp Val Asn Asn Ile  Lys Asp Glu Lys Ser  Leu Ile Lys 
    1145                 1150                 1155             


Tyr Leu  Val Glu Asn Lys Lys  Leu Val Glu Pro Arg  Val Ile Lys 
    1160                 1165                 1170             


Lys Val  Tyr Lys Arg Gln Val  Ile Leu Ile Asn Asp  Tyr Pro Tyr 
    1175                 1180                 1185             


Ser Ile  Val Ala Leu Asp Ser  Asn Lys Leu Met Asp  Phe Glu Asn 
    1190                 1195                 1200             


Leu Lys  Pro Leu Phe Leu Glu  Arg Lys Tyr Glu Lys  Ile Leu Lys 
    1205                 1210                 1215             


Asn Val  Ile Lys Phe Leu Glu  Asp Asn Gln Gly Lys  Ser Glu Glu 
    1220                 1225                 1230             


Asn Tyr  Lys Phe Ile Tyr Leu  Lys Lys Lys Asp Arg  Tyr Glu Lys 
    1235                 1240                 1245             


Asn Glu  Thr Leu Glu Ser Val  Arg Glu Arg Tyr Asn  Ile Glu Phe 
    1250                 1255                 1260             


Asn Glu  Met Tyr Asp Lys Phe  Leu Glu Lys Leu Asn  Ser Lys Asp 
    1265                 1270                 1275             


Tyr Lys  Asn Tyr Ile Asn Asn  Lys Lys Tyr Gln Asp  Leu Leu Asp 
    1280                 1285                 1290             


Val Lys  Glu Lys Phe Ile Lys  Leu Asn Leu Phe Asp  Lys Ala Phe 
    1295                 1300                 1305             


Thr Leu  Lys Ser Phe Leu Asp  Leu Phe Asn Arg Lys  Thr Met Ala 
    1310                 1315                 1320             


Asp Phe  Ser Lys Val Gly Leu  Thr Lys Tyr Leu Gly  Lys Ile Gln 
    1325                 1330                 1335             


Lys Ile  Ser Ser Asn Val Leu  Ser Lys Asn Glu Leu  Tyr Leu Leu 
    1340                 1345                 1350             


Glu Glu  Ser Val Thr Gly Leu  Phe Val Lys Lys Ile  Glu Leu 
    1355                 1360                 1365         


<210>  989
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  989

Met Val Lys Lys Lys Tyr Ala Ile Gly Ile Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Val Val Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asn Thr Glu Lys Arg Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Thr Leu Leu Phe Asp Glu Gly Asn Thr Ala Glu Asn Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile 
65                  70                  75                  80  


Leu Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Lys Ile Asp Asp 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Ile Val Glu Asp Lys 
            100                 105                 110         


Gln Gly Ser Lys His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Ser Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Lys 
                165                 170                 175     


Ser Glu Asn Lys Asp Val Gln His Leu Phe Asn Asp Phe Val Glu Met 
            180                 185                 190         


Phe Asp Lys Thr Val Glu Gly Ser Tyr Leu Ser Glu Asn Leu Pro Asn 
        195                 200                 205             


Val Ala Asp Val Leu Val Glu Lys Val Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Ile Leu His Tyr Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly 
225                 230                 235                 240 


Asn Phe Leu Thr Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Glu Leu Ala Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Ile Ala Thr Lys Ser Leu Tyr Asp Gly Ile Leu Leu Ala 
    290                 295                 300                 


Gly Ile Leu Ser Thr Thr Asp Ser Thr Thr Lys Ala Pro Leu Ser Ser 
305                 310                 315                 320 


Ser Met Val Asn Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu 
                325                 330                 335     


Lys Asn Phe Ile His Gln Asn Leu Ser Asp Ser Tyr Lys Glu Val Phe 
            340                 345                 350         


Asn Asp Lys Leu Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Asn Phe Tyr Arg Phe Ile Lys Lys Ala Ile Glu Lys Ile 
    370                 375                 380                 


Glu Gly Ser Asn Tyr Phe Ile Asp Lys Ile Asp Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro 
            420                 425                 430         


Phe Leu Val Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Glu Phe Ala 
    450                 455                 460                 


Trp Leu Asn Arg Lys Ser Asp Glu Lys Ile Arg Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Glu Met Val Asp Lys Glu Thr Ser Ala Glu Asn Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Asn Tyr Asp Gln Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Val Thr Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr 
545                 550                 555                 560 


Lys Glu Lys Leu Met Asp Phe Leu Gly Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Leu Leu Gly Leu Asp Lys Asp Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Val Ser Lys Asp Leu 
        595                 600                 605             


Leu Asp Asn Pro Glu Asn Glu Asp Ile Leu Glu Asn Val Val Leu Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys 
625                 630                 635                 640 


Tyr Lys Asp Val Leu Thr Glu Glu Gln Arg Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Val Thr Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Thr Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu 
    690                 695                 700                 


Ser Phe Val Asp Glu Ile Arg Leu Ala Gln Gly Ser Gly Glu Ala Glu 
705                 710                 715                 720 


Asp Tyr Arg Ala Glu Val Gln Asn Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Ile Glu Val 
            740                 745                 750         


Met Gly Tyr Asp Pro Glu His Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Lys 
    770                 775                 780                 


Ile Glu Asn Ala Ile Lys Asn Leu Asp Ser Asn Leu Asn Ser Lys Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asn Asn Gln Ala Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Glu Glu 
            820                 825                 830         


Leu Asp Ile Asp Gln Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Thr Lys 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val His Lys Lys Lys Asn Phe Trp Lys Gln Leu Leu Asp Ser Gln Leu 
                885                 890                 895     


Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Asn Glu Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Thr Arg Phe 
    930                 935                 940                 


Asn Thr Lys Leu Asp Glu Ala Gly Asn Arg Ile Arg Asp Pro Lys Val 
945                 950                 955                 960 


Asn Ile Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Tyr Gln Leu Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Thr Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Gln  Leu Ala Pro Glu Phe  Val Tyr Gly Asp Tyr  Pro Lys Tyr 
    1010                 1015                 1020             


Asn Ser  Tyr Lys Ser Arg Lys  Ser Ala Thr Glu Lys  Val Leu Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Arg Arg Val Leu  Val Tyr Ser 
    1040                 1045                 1050             


Lys Thr  Gly Glu Val Arg Ile  Arg Pro Val Ile Glu  Val Asn Lys 
    1055                 1060                 1065             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Lys Ser Asp  Phe Arg Thr 
    1070                 1075                 1080             


Val Arg  Lys Val Leu Ser Tyr  Pro Gln Val Asn Val  Val Lys Lys 
    1085                 1090                 1095             


Val Glu  Met Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Gln 
    1100                 1105                 1110             


His Gly  Asp Ser Asp Lys Leu  Ile Pro Arg Lys Thr  Glu Lys Phe 
    1115                 1120                 1125             


Tyr Leu  Asp Thr Lys Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Ile 
    1130                 1135                 1140             


Ala Tyr  Ser Val Leu Leu Ile  Ala Asp Ile Glu Lys  Gly Lys Ala 
    1145                 1150                 1155             


Lys Lys  Leu Lys Arg Val Lys  Glu Leu Ile Gly Ile  Thr Ile Met 
    1160                 1165                 1170             


Glu Arg  Met Ala Phe Glu Lys  Asn Pro Ile Glu Phe  Leu Glu His 
    1175                 1180                 1185             


Lys Gly  Tyr Lys Asn Ile Leu  Glu Lys Asn Ile Ile  Lys Leu Pro 
    1190                 1195                 1200             


Lys Tyr  Ser Leu Phe Glu Leu  Glu Asn Gly Arg Arg  Arg Leu Leu 
    1205                 1210                 1215             


Ala Ser  Ala Lys Glu Leu Gln  Lys Gly Asn Glu Met  Ile Leu Pro 
    1220                 1225                 1230             


Pro His  Leu Val Thr Leu Leu  Tyr His Ser Ser Asn  Ile His Lys 
    1235                 1240                 1245             


Ile Thr  Glu Pro Ile His Leu  Asn Tyr Val Asn Lys  Asn Lys His 
    1250                 1255                 1260             


Glu Phe  Lys Glu Leu Leu Arg  His Ile Ser Asp Phe  Ser Thr Arg 
    1265                 1270                 1275             


Tyr Ile  Leu Ala Gln Asp Arg  Leu Ser Lys Ile Glu  Glu Leu Tyr 
    1280                 1285                 1290             


Asp Lys  Asn Asp Gly Asp Asp  Ile Ser Asp Leu Thr  Ser Ser Phe 
    1295                 1300                 1305             


Val Asn  Leu Leu Thr Phe Thr  Ala Ile Gly Ala Pro  Ala Ala Phe 
    1310                 1315                 1320             


Lys Phe  Leu Gly Ser Val Ile  Asp Arg Lys Arg Tyr  Thr Ser Ile 
    1325                 1330                 1335             


Ala Glu  Ile Leu Glu Ala Thr  Leu Ile His Gln Ser  Val Thr Gly 
    1340                 1345                 1350             


Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Lys Leu Gly  Gly Asp 
    1355                 1360                 1365         


<210>  990
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  990

Met Val Lys Lys Lys Tyr Ala Ile Gly Ile Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Val Val Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asn Thr Glu Lys Arg Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Thr Leu Leu Phe Asp Glu Gly Asn Thr Ala Glu Asn Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile 
65                  70                  75                  80  


Leu Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Lys Ile Asp Asp 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Ile Val Glu Asp Lys 
            100                 105                 110         


Gln Gly Ser Lys His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Ser Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Lys 
                165                 170                 175     


Ser Glu Asn Lys Asp Val Gln His Leu Phe Asn Asp Phe Val Glu Met 
            180                 185                 190         


Phe Asp Lys Thr Val Glu Gly Ser Tyr Leu Ser Glu Asn Leu Pro Asn 
        195                 200                 205             


Val Ala Asp Val Leu Val Glu Lys Val Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Ile Leu His Tyr Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly 
225                 230                 235                 240 


Asn Phe Leu Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Glu Leu Ala Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Ile Ala Thr Lys Ser Leu Tyr Asp Gly Ile Leu Leu Ala 
    290                 295                 300                 


Gly Ile Leu Ser Thr Thr Asp Ser Thr Thr Lys Ala Pro Leu Ser Ser 
305                 310                 315                 320 


Ser Met Val Asn Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu 
                325                 330                 335     


Lys Asn Phe Ile His Gln Asn Leu Ser Asp Ser Tyr Lys Glu Val Phe 
            340                 345                 350         


Asn Asp Lys Leu Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Asn Phe Tyr Arg Phe Ile Lys Lys Ala Ile Glu Lys Ile 
    370                 375                 380                 


Glu Gly Ser Asn Tyr Phe Ile Asp Lys Ile Asp Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro 
            420                 425                 430         


Phe Leu Val Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Glu Phe Ala 
    450                 455                 460                 


Trp Leu Asn Arg Lys Ser Asp Glu Lys Ile Arg Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Glu Met Val Asp Lys Glu Thr Ser Ala Glu Asn Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Asn Tyr Asp Gln Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Val Thr Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr 
545                 550                 555                 560 


Lys Glu Lys Leu Met Asp Phe Leu Gly Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Leu Leu Gly Leu Asp Lys Asp Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Val Ser Lys Asp Leu 
        595                 600                 605             


Leu Asp Asn Pro Glu Asn Glu Asp Ile Leu Glu Asn Val Val Leu Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys 
625                 630                 635                 640 


Tyr Lys Asp Val Leu Thr Glu Glu Gln Arg Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Val Thr Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Thr Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu 
    690                 695                 700                 


Ser Phe Val Asp Glu Ile Arg Leu Ala Gln Gly Ser Gly Glu Ala Glu 
705                 710                 715                 720 


Asp Tyr Arg Ala Glu Val Gln Asn Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Ile Glu Val 
            740                 745                 750         


Met Gly Tyr Asp Pro Glu His Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Lys 
    770                 775                 780                 


Ile Glu Asn Ala Ile Lys Asn Leu Asp Ser Asn Leu Asn Ser Lys Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asn Asn Gln Ala Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Glu Glu 
            820                 825                 830         


Leu Asp Ile Asp Gln Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Thr Lys 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val His Lys Lys Lys Asn Phe Trp Lys Gln Leu Leu Asp Ser Gln Leu 
                885                 890                 895     


Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Asn Glu Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Thr Arg Phe 
    930                 935                 940                 


Asn Thr Lys Leu Asp Glu Ala Gly Asn Arg Ile Arg Asp Pro Lys Val 
945                 950                 955                 960 


Asn Ile Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Tyr Gln Leu Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Thr Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Gln  Leu Ala Pro Glu Phe  Val Tyr Gly Asp Tyr  Pro Lys Tyr 
    1010                 1015                 1020             


Asn Ser  Tyr Lys Ser Arg Lys  Ser Ala Thr Glu Lys  Val Leu Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Arg Arg Val Leu  Val Tyr Ser 
    1040                 1045                 1050             


Lys Thr  Gly Glu Val Arg Ile  Arg Pro Val Ile Glu  Val Asn Lys 
    1055                 1060                 1065             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Lys Ser Asp  Phe Arg Thr 
    1070                 1075                 1080             


Val Arg  Lys Val Leu Ser Tyr  Pro Gln Val Asn Val  Val Lys Lys 
    1085                 1090                 1095             


Val Glu  Met Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Gln 
    1100                 1105                 1110             


His Gly  Asp Ser Asp Lys Leu  Ile Pro Arg Lys Thr  Glu Lys Phe 
    1115                 1120                 1125             


Tyr Leu  Asp Thr Lys Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Ile 
    1130                 1135                 1140             


Ala Tyr  Ser Val Leu Leu Ile  Ala Asp Ile Glu Lys  Gly Lys Ala 
    1145                 1150                 1155             


Lys Lys  Leu Lys Arg Val Lys  Glu Leu Ile Gly Ile  Thr Ile Met 
    1160                 1165                 1170             


Glu Arg  Met Ala Phe Glu Lys  Asn Pro Ile Glu Phe  Leu Glu His 
    1175                 1180                 1185             


Lys Gly  Tyr Lys Asn Ile Leu  Glu Lys Asn Ile Ile  Lys Leu Pro 
    1190                 1195                 1200             


Lys Tyr  Ser Leu Phe Glu Leu  Glu Asn Gly Arg Arg  Arg Leu Leu 
    1205                 1210                 1215             


Ala Ser  Ala Lys Glu Leu Gln  Lys Gly Asn Glu Met  Ile Leu Pro 
    1220                 1225                 1230             


Pro His  Leu Val Thr Leu Leu  Tyr His Ser Ser Asn  Ile His Lys 
    1235                 1240                 1245             


Ile Thr  Glu Pro Ile His Leu  Asn Tyr Val Asn Lys  Asn Lys His 
    1250                 1255                 1260             


Glu Phe  Lys Glu Leu Leu Arg  His Ile Ser Asp Phe  Ser Thr Arg 
    1265                 1270                 1275             


Tyr Ile  Leu Ala Gln Asp Arg  Leu Ser Lys Ile Glu  Glu Leu Tyr 
    1280                 1285                 1290             


Asp Lys  Asn Asp Gly Asp Asp  Ile Ser Asp Leu Thr  Ser Ser Phe 
    1295                 1300                 1305             


Val Asn  Leu Leu Thr Phe Thr  Ala Ile Gly Ala Pro  Ala Ala Phe 
    1310                 1315                 1320             


Lys Phe  Leu Gly Ser Val Ile  Asp Arg Lys Arg Tyr  Thr Ser Ile 
    1325                 1330                 1335             


Ala Glu  Ile Leu Glu Ala Thr  Leu Ile His Gln Ser  Val Thr Gly 
    1340                 1345                 1350             


Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Lys Leu Gly  Gly Asp 
    1355                 1360                 1365         


<210>  991
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  991

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Phe Val Gly Val Asp Glu Asn Gly Trp Pro Leu Asn Ile Lys Gly 
            20                  25                  30          


Lys Arg Ala Ile Gly Ala His Leu Phe Arg Glu Gly Glu Ala Ala Ala 
        35                  40                  45              


Glu Thr Arg Gly Phe Arg Thr Thr Arg Arg Arg Tyr Asn Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Ile Phe Asp Glu Pro Ile Ser Arg 
65                  70                  75                  80  


Val Asp Pro Thr Phe Phe Ala Arg Met Lys Glu Ser Ser Val Ser Pro 
                85                  90                  95      


Lys Asp Pro Asn Tyr Asp Gly Leu Ala Lys Glu Ile Leu Ile Pro Arg 
            100                 105                 110         


Asp Lys Asp Gly Lys Ile Leu Tyr Pro Thr Ile Tyr His Leu Arg His 
        115                 120                 125             


Ala Leu Met Tyr Glu Asp His Gln Phe Asp Ile Arg Glu Ile Tyr Leu 
    130                 135                 140                 


Ala Ile His His Ile Val Lys Tyr Arg Gly His Phe Leu Leu Asp Gly 
145                 150                 155                 160 


Ala Ala Lys Asn Phe Glu Ser Ser Asn Leu Asp Leu Lys Ser Asp Ile 
                165                 170                 175     


His Glu Ile Asn Ala Ile Leu Val Gly Ile Phe Gly Asn Glu Gln Val 
            180                 185                 190         


Gln Phe Asn Asp Gln Tyr Val Glu Lys Leu Lys Glu Val Leu Leu Asp 
        195                 200                 205             


Ser Thr Gln Ser Arg Ser Asp Arg Gln Lys Ala Ala Ala Lys Val Leu 
    210                 215                 220                 


Leu Pro Thr Asn Asp Leu Asp Lys Asp Thr Thr Lys Ile Leu Lys Thr 
225                 230                 235                 240 


Val Ser Thr Glu Ile Thr Lys Ala Ile Thr Gly Leu Lys Ala Lys Phe 
                245                 250                 255     


Phe Val Ile Thr Gly Asn Asp Ile Ala Lys Asp Gln Gln Lys Asp Trp 
            260                 265                 270         


Thr Phe Ser Ile Asp Thr Leu Asp Glu Lys Met Ser Val Ile Glu Pro 
        275                 280                 285             


Ser Phe Ser Glu Lys Asn Phe Ser Leu Ile Thr Ile Leu Gln Lys Leu 
    290                 295                 300                 


Tyr Ser Ser Val Thr Leu Ala Gly Ile Val Pro Asn Gly Leu Ser Leu 
305                 310                 315                 320 


Ser Glu Ala Met Ile Gln Lys Tyr Asp Arg His Ala Ser Asp Leu Val 
                325                 330                 335     


Leu Leu Lys Lys Tyr Trp Asp Thr Lys Pro Lys Val Leu Lys His Gln 
            340                 345                 350         


Leu Gln Asn Thr Tyr Ala Glu Tyr Ile Glu Ile His Gln Val Val Asp 
        355                 360                 365             


Glu Phe Tyr Ser Asp Leu Ser Lys Leu Ile Lys Asn Asp Asp Asp Thr 
    370                 375                 380                 


Leu Ala Gln Glu Ile Lys Ser Lys Ile Thr Ile Gly Asn Phe Leu Pro 
385                 390                 395                 400 


Lys Gln Arg Thr Thr Glu Asn Gly Val Ile Pro Tyr Gln Leu Gln Gln 
                405                 410                 415     


Arg Glu Leu Asp Thr Ile Ile Lys Asn Gln Gln Glu Tyr Tyr Pro Trp 
            420                 425                 430         


Leu Ala Glu Glu Asn Pro Val Ala Asp His Gln Ile Ala Phe Pro Tyr 
        435                 440                 445             


Lys Leu Asp Glu Leu Val Gly Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
    450                 455                 460                 


Met Ile Ala Lys Glu Asp Thr Ser Asn Ser Lys Asn Ser Lys Phe Ala 
465                 470                 475                 480 


Trp Met Ile Arg Lys Gln Ser Gly Glu Val Thr Pro Trp Asn Phe Asp 
                485                 490                 495     


Glu Lys Ile Asp Arg Thr Ala Ser Ala Thr Ala Phe Ile Asp Arg Met 
            500                 505                 510         


Thr Thr Thr Asp Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Gln 
        515                 520                 525             


Ser Leu Ile Tyr Gln Arg Phe Glu Val Leu Asn Glu Leu Asn Lys Ile 
    530                 535                 540                 


Ser Ile Asp Gly Gln Pro Ile Thr Arg Lys Gln Lys Gln Ser Val Tyr 
545                 550                 555                 560 


Thr His Val Phe Glu Lys Lys Lys Thr Val Gln Val Lys Asp Val Gln 
                565                 570                 575     


Lys Phe Leu Val Ser Ser Gly Glu Val Met Ser Leu Pro Lys Ile Gly 
            580                 585                 590         


Gly Leu Ala Asn Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr Leu Tyr 
        595                 600                 605             


Asp Tyr Asn Lys Ile Ile Pro Asp Ala Leu Glu Asp Arg Asn Lys Val 
    610                 615                 620                 


Asp Asp Ile Glu Lys Ile Ile Leu Trp Ser Thr Ile Phe Glu Asp Asn 
625                 630                 635                 640 


Ser Ile Phe Val Glu Lys Leu Asp Ser Ile Asp Trp Leu Thr Ser Ser 
                645                 650                 655     


Gln Arg Lys Arg Leu Ala Gln Lys Arg Tyr Arg Gly Trp Gly Arg Leu 
            660                 665                 670         


Ser Leu Arg Leu Leu Thr Thr Phe Lys Asn Lys Asp Gly Tyr Ser Ile 
        675                 680                 685             


Leu Asp Glu Leu Trp Asn Ser Asn His Asn Phe Met Glu Ile Gln Ala 
    690                 695                 700                 


Gln Ser Asp Phe Ser Glu Gln Ile Ala Thr Ala Asn Asn Asn Asn Leu 
705                 710                 715                 720 


Ser Gln Val Leu Pro Ala Lys Trp Glu Pro Gly Arg Lys Asn Leu Ala 
                725                 730                 735     


Ser Leu Asp Glu Phe Ile Ser Asn Leu Tyr Thr Ser Pro Gln Asn Lys 
            740                 745                 750         


Lys Ala Leu Arg Lys Ala Ile Arg Val Val Gln Asp Met Met Thr Ala 
        755                 760                 765             


Ala Gly His Met Pro Glu Lys Ile Phe Ile Glu Val Ala Arg Asp Thr 
    770                 775                 780                 


Ser Asn Ser His Gln Gln Thr Ile Ala Arg Gln Arg Gln Leu Gln Gln 
785                 790                 795                 800 


Leu Tyr Gln Ser Glu Ala Lys Glu Val Ile Asp Ser Ser Val Arg Ser 
                805                 810                 815     


Glu Leu Asp Asp Ile Leu Lys Asn Arg Ala Asn Ile Lys Asp Gln Leu 
            820                 825                 830         


Phe Leu Tyr Phe Gln Gln Gly Gly Arg Asp Leu Tyr Thr Gly Lys Ala 
        835                 840                 845             


Ile Asn Ile Asp Lys Leu Ser Leu Tyr His Ile Asp His Ile Leu Pro 
    850                 855                 860                 


Gln Ser Leu Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser 
865                 870                 875                 880 


Gln Lys Thr Asn Glu Asp Lys Gly Val Thr Phe Val Ser Asp Lys Tyr 
                885                 890                 895     


Met Asn Asn Met Leu Pro Thr Trp Lys Arg Trp His Glu Met Gly Leu 
            900                 905                 910         


Ile Ser Ala Arg Lys Leu Lys Asn Leu Thr Met Arg Pro Asp Glu Ile 
        915                 920                 925             


Asp Lys Tyr Ala Pro Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg 
    930                 935                 940                 


Gln Val Thr Lys Leu Val Ile Glu Leu Leu Asp Ala Leu Leu Gly Asn 
945                 950                 955                 960 


Ser Gln Thr Glu Ile Ile Thr Ile Lys Ala Ser Leu Ser His Gln Phe 
                965                 970                 975     


Arg Gln Gln Phe Asp Leu Val Lys Ile Arg Glu Val Asn Asp Asn His 
            980                 985                 990         


His Ala Phe Asp Ala Phe Leu Ala  Ala Arg Ile Gly Thr  Tyr Leu Tyr 
        995                 1000                 1005             


Lys Arg  Tyr Pro Lys Leu Arg  Pro Tyr Phe Val Tyr  Gly Gln Tyr 
    1010                 1015                 1020             


Met Gln  Asn His Lys Asn Leu  Lys Ser Ile Asn Leu  Ile Lys Thr 
    1025                 1030                 1035             


Leu Asp  Ser Asp Lys Ala Ile  Val Asp Glu Gly Thr  Gly Glu Ile 
    1040                 1045                 1050             


Leu Trp  Asp Lys Lys Ser Asp  Leu Ala Lys Met Ile  Lys Ile Tyr 
    1055                 1060                 1065             


Asn Tyr  Gln Arg Leu Leu Ile  Thr His Glu Ser Arg  Thr Ser Phe 
    1070                 1075                 1080             


Gly Ala  Met Tyr Lys Gln Lys  Ile Leu Lys Ala Thr  Asp Ala Lys 
    1085                 1090                 1095             


Gln Asn  Gly Gly Gly Phe Lys  Leu Val Pro Thr Lys  Arg Asn Trp 
    1100                 1105                 1110             


Pro Val  Asp Ile Tyr Gly Gly  Tyr Thr Gly Gln Thr  Ala Ala Tyr 
    1115                 1120                 1125             


Leu Thr  Ile Val Arg Val Tyr  Ser Lys Lys Thr Ser  Glu Leu Arg 
    1130                 1135                 1140             


Val Leu  Pro Ile Tyr Thr Asn  Lys Val Gln Gln Ile  Glu Lys Ala 
    1145                 1150                 1155             


Met Thr  Gln Gly Asp Lys Ala  Thr Met Leu Ala Phe  Lys Lys Val 
    1160                 1165                 1170             


Ile Ala  Pro Gln Leu Gly Lys  Glu Asn Lys Lys Thr  His Glu Ile 
    1175                 1180                 1185             


Lys Ile  Pro Glu Phe Asp Val  Val Ile Pro Lys Val  Leu Leu Asp 
    1190                 1195                 1200             


Gln Leu  Val Ile Asp Asp Ile  Ala Gly Ser Pro His  Arg Phe Arg 
    1205                 1210                 1215             


Leu Gly  Thr Asp Thr Tyr Tyr  His Asn Ser Gln Gln  Leu Phe Leu 
    1220                 1225                 1230             


Pro Leu  Ser Met Gln Arg Gln  Leu Met Asp Arg Glu  Ser Asp Asp 
    1235                 1240                 1245             


Lys Val  Tyr Leu Asp Ile Phe  Asn Asp Val Leu Lys  Gln Val Asp 
    1250                 1255                 1260             


Ser Tyr  Phe Asn Leu Tyr Asp  Val Asn Gly Phe Arg  Ala Ala Leu 
    1265                 1270                 1275             


Gln Lys  Ser Lys Lys Ala Phe  Glu Gln Leu Pro Ile  Asn Asp Phe 
    1280                 1285                 1290             


Pro Lys  Asp Val Pro Asp Asn  Lys Lys Lys Lys Gly  Lys Arg Ser 
    1295                 1300                 1305             


Ile Leu  Lys Glu Ile Phe Ile  Gly Leu His Ala Asn  Ala Thr Leu 
    1310                 1315                 1320             


Gly Asn  Leu Lys Gln Met Gly  Ile Lys Thr Asp Phe  Gly Lys Leu 
    1325                 1330                 1335             


Gln Lys  Ser Asn Gly Leu Arg  Ile Thr Glu Asn Cys  Lys Ile Ile 
    1340                 1345                 1350             


His Gln  Ser Pro Thr Gly Leu  Phe Glu Arg Ile Val  Pro Ile Lys 
    1355                 1360                 1365             


<210>  992
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  992

Met Ser Arg Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Trp Ser Val Val Asp Asp Gln Ser Lys Leu Val Ser Val Arg Gly 
            20                  25                  30          


Lys Tyr Gly Tyr Gly Val Arg Leu Tyr Asp Glu Gly Gln Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Gly Leu Leu Arg Glu Ile Phe Glu Pro Tyr Ile Thr Pro 
65                  70                  75                  80  


Val Asp Asp Thr Phe Phe Leu Arg Gln Lys Gln Ser Asn Leu Ser Pro 
                85                  90                  95      


Lys Asp Gln Arg Lys Leu Tyr Pro Gln Thr Ser Leu Phe Asn Asp Arg 
            100                 105                 110         


Thr Asp Arg Ala Phe Tyr Asp Asp Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Tyr Lys Leu Met Thr Glu Lys Arg Gln Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Met His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn Glu 
145                 150                 155                 160 


Ala Pro Val Ser Ser Phe Lys Ser Ser Glu Ile Asn Leu Val Ala His 
                165                 170                 175     


Phe Asp Arg Leu Asn Thr Ile Phe Ala Asp Leu Phe Ser Glu Ser Gly 
            180                 185                 190         


Phe Gln Leu Glu Thr Asp Lys Leu Ala Glu Val Lys Ala Leu Leu Leu 
        195                 200                 205             


Asp Asn Gln Gln Ser Ala Ser Asn Arg Gln Arg Gln Ala Leu Ser Leu 
    210                 215                 220                 


Ile Tyr Thr Pro Ser Thr Asn Lys Ala Val Glu Lys Gln Asn Lys Ala 
225                 230                 235                 240 


Ile Ala Thr Glu Leu Leu Lys Ala Ile Leu Gly Leu Lys Ala Lys Phe 
                245                 250                 255     


Asn Val Leu Thr Gly Ile Glu Ala Glu Asp Val Lys Ala Trp Thr Leu 
            260                 265                 270         


Thr Phe Asn Ala Glu Asn Phe Asp Glu Glu Met Val Lys Leu Glu Ser 
        275                 280                 285             


Ser Leu Asp Asp Asn Ala His Gln Ile Ile Glu Ser Leu Gln Glu Leu 
    290                 295                 300                 


Tyr Ser Gly Val Leu Leu Ala Gly Ile Val Pro Glu Asn Gln Ser Leu 
305                 310                 315                 320 


Ser Gln Ala Met Ile Thr Lys Tyr Asp Asp His Gln Lys His Leu Lys 
                325                 330                 335     


Met Leu Lys Ala Val Arg Glu Ala Leu Ala Pro Glu Asp Arg Gln Arg 
            340                 345                 350         


Leu Lys Gln Ala Tyr Asp Gln Tyr Val Asp Gly Gln Glu Asn Thr Lys 
        355                 360                 365             


Ala Tyr Ser Lys Glu Asp Phe Tyr Gly Asp Ile Thr Lys Ala Leu Lys 
    370                 375                 380                 


Asn Asn Pro Asp His Pro Ile Val Ser Glu Ile Lys Lys Leu Ile Glu 
385                 390                 395                 400 


Leu Asp Gln Phe Met Pro Lys Gln Arg Thr Lys Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Gln Gln Glu Leu Asp Arg Ile Ile Glu Asn Gln 
            420                 425                 430         


Gln Gln Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Ser Lys Arg 
        435                 440                 445             


Gln Thr Val Ala Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ile Thr Ala Glu Gln Gln Arg Gln Ser 
465                 470                 475                 480 


Ser Asp Ala Lys Phe Ala Trp Leu Ile Arg Lys Ala Glu Gly Arg Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Asp Lys Val Asp Arg Gln Ala Ser Ala Asn 
            500                 505                 510         


Glu Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu Ala Glu 
        515                 520                 525             


Asp Val Leu Pro Lys Gln Ser Leu Ile Tyr Gln Arg Phe Glu Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Gly Leu Lys Ile Asp Asp Gln Pro Ile Thr Thr Glu 
545                 550                 555                 560 


Leu Lys Gln Ala Ile Phe Thr Asp Leu Phe Met Gln Lys Ile Ser Val 
                565                 570                 575     


Thr Val Lys Asn Ile Gln Asp Tyr Leu Val Ser Glu Lys Arg Tyr Ala 
            580                 585                 590         


Ser Arg Pro Ala Ile Thr Gly Leu Ser Asp Glu Asn Lys Phe Asn Ser 
        595                 600                 605             


Arg Leu Ser Thr Tyr His Asp Leu Lys Met Ile Val Gly Asp Ala Val 
    610                 615                 620                 


Asp Asp Val Asp Lys Gln Ala Asp Leu Glu Lys Cys Ile Glu Trp Ser 
625                 630                 635                 640 


Thr Ile Phe Glu Asp Gly Lys Ile Tyr Ser Ala Lys Leu Asn Glu Ile 
                645                 650                 655     


Asp Trp Leu Thr Asp Gln Gln Arg Val Gln Leu Ala Ala Lys Arg Tyr 
            660                 665                 670         


Arg Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Thr Gln Ile Val Asn 
        675                 680                 685             


Ala Asn Gly Gln Arg Ile Met Asp Leu Leu Trp Asp Thr Thr Asp Asn 
    690                 695                 700                 


Phe Met Arg Ile Val His Ser Glu Asp Phe Asp Lys Leu Ile Thr Glu 
705                 710                 715                 720 


Ala Asn Gln Met Met Leu Ala Glu Asn Asp Val Gln Asp Val Ile Asn 
                725                 730                 735     


Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Ile Leu 
            740                 745                 750         


Leu Val Val Asn Asp Ile Gln Lys Ala Met Lys Gly Gln Ala Pro Glu 
        755                 760                 765             


Arg Ile Leu Ile Glu Phe Ala Arg Glu Asp Glu Val Asn Pro Arg Leu 
    770                 775                 780                 


Ser Val Gln Arg Lys Arg Gln Val Glu Gln Val Tyr Gln Asn Ile Ser 
785                 790                 795                 800 


Asn Glu Leu Leu Asn Asn Thr Glu Ile Arg Asn Glu Leu Lys Asp Leu 
                805                 810                 815     


Ser Asn Ser Ala Leu Ser Asn Thr Arg Leu Phe Leu Tyr Phe Met Gln 
            820                 825                 830         


Gly Gly Arg Asp Met Tyr Thr Gly Asp Ser Leu Asn Ile Asp Arg Leu 
        835                 840                 845             


Ser Thr Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe Val Lys Asp 
    850                 855                 860                 


Asn Ser Leu Asp Asn Arg Val Leu Val Ser Gln Lys Met Asn Arg Ser 
865                 870                 875                 880 


Lys Ala Asp Gln Val Pro Thr Asp Phe Thr Ser Val Glu Leu Gly Lys 
                885                 890                 895     


Lys Met Gln Leu Gln Trp Glu Gln Met Leu Arg Ala Gly Leu Ile Thr 
            900                 905                 910         


Lys Lys Lys Tyr Asp Asn Leu Thr Leu Asn Pro Asp His Ile Ser Lys 
        915                 920                 925             


Tyr Ala Met Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Val Ile Lys Leu Ala Thr Asn Leu Leu Met Glu Gln Tyr Gly Glu Asp 
945                 950                 955                 960 


Asn Ile Glu Leu Ile Thr Val Lys Ser Gly Leu Thr His Gln Met Arg 
                965                 970                 975     


Thr Glu Phe Asp Phe Pro Lys Asn Arg Asn Leu Asn Asn His His His 
            980                 985                 990         


Ala Phe Asp Ala Tyr Leu Thr Ala  Phe Val Gly Leu Tyr  Leu Leu Lys 
        995                 1000                 1005             


Arg Tyr  Pro Lys Leu Lys Pro  Tyr Phe Val Tyr Gly  Glu Tyr Gln 
    1010                 1015                 1020             


Lys Ala  Ser Gln Gln Asp Lys  Trp Arg Asn Phe Asn  Phe Leu Asn 
    1025                 1030                 1035             


Gly Leu  Lys Lys Asp Glu Leu  Val Asp Glu Asn Thr  Glu Ala Val 
    1040                 1045                 1050             


Ile Trp  Asp Lys Glu Ser Gly  Leu Ala Tyr Leu Asn  Lys Ile Tyr 
    1055                 1060                 1065             


Gln Phe  Lys Lys Ile Leu Val  Thr Arg Glu Val His  Glu Asn Ser 
    1070                 1075                 1080             


Gly Ala  Leu Phe Asn Gln Thr  Leu Tyr Ala Ala Lys  Asp Asp Lys 
    1085                 1090                 1095             


Ala Ser  Gly Gln Gly Gly Lys  Gln Leu Ile Pro Ala  Lys Gln Asp 
    1100                 1105                 1110             


Arg Pro  Thr Ala Leu Tyr Gly  Gly Tyr Ser Gly Lys  Thr Ser Ala 
    1115                 1120                 1125             


Tyr Leu  Cys Leu Val Lys Ile  Lys Asn Lys Ser Glu  Tyr Ile Tyr 
    1130                 1135                 1140             


Arg Ile  Phe Gly Val Asn Thr  Ser Trp Ser Ser Glu  Leu Lys Gln 
    1145                 1150                 1155             


Ile Gln  Asp Pro Ala Glu Arg  Leu Val Arg Leu Glu  Lys Leu Leu 
    1160                 1165                 1170             


Thr Pro  Gln Leu Thr Ile Asn  Lys Lys Lys Lys Gly  Glu Pro Val 
    1175                 1180                 1185             


Pro Val  Thr Lys Asp Phe Lys  Ile Ile Leu Pro Arg  Ile Leu Ile 
    1190                 1195                 1200             


Asn Gln  Lys Phe Tyr Ser Arg  Gly Gln Asp Ile Ser  Ile Ala Ser 
    1205                 1210                 1215             


Glu Phe  Tyr Leu His Asn Glu  Gln Glu Leu Val Leu  Asp Lys Ser 
    1220                 1225                 1230             


Asp Ile  Lys Ile Leu Asn Gly  Ala Ile Lys Val Asn  Asn Ser Asn 
    1235                 1240                 1245             


Lys Gln  Val Ile Asn Leu Phe  Asn His Ile Leu Glu  Gln Val Asn 
    1250                 1255                 1260             


Gln Tyr  Phe Pro Val Tyr Asp  Ile Tyr Lys Ser Arg  Glu Val Leu 
    1265                 1270                 1275             


Asn Ser  Ser Lys Glu Ile Phe  Glu Asn Leu Thr Trp  Asn Asp Gln 
    1280                 1285                 1290             


Trp Arg  Asn Arg Lys Leu Ile  Asn Val Gly Gln Lys  Thr Ile Leu 
    1295                 1300                 1305             


Asn Arg  Ile Leu Ile Gly Leu  His Ala Asn Ala Ala  Arg Ile Lys 
    1310                 1315                 1320             


Ile Ser  Glu Leu Gly Phe Lys  Asn Asp Phe Gly Leu  Leu Thr Gln 
    1325                 1330                 1335             


Thr Ser  Gly Ile Thr Phe Thr  Pro Asp Thr Gln Ile  Ile Tyr Gln 
    1340                 1345                 1350             


Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Val Ala Leu  Arg Asp Leu 
    1355                 1360                 1365             


<210>  993
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  993

Met Lys Arg Ser Tyr Asn Ile Gly Leu Asp Ile Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Trp Ala Ile Val Asp Asp Gln Ser Lys Leu Ile Ser Val Lys Gly 
            20                  25                  30          


Lys Tyr Gly Tyr Gly Val Arg Leu Tyr Asp Glu Gly Gln Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Gly Leu Leu Arg Glu Ile Phe Glu Pro Tyr Ile Thr Pro 
65                  70                  75                  80  


Ile Asp Ser Thr Phe Phe Leu Arg Gln Lys Gln Ser Asn Leu Ser Pro 
                85                  90                  95      


Lys Asp Gln Arg Lys Leu Tyr Pro Gln Thr Ser Leu Phe Asn Asp Arg 
            100                 105                 110         


Pro Asp Ala Asp Phe Tyr Gln Gln Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Tyr Gln Leu Met Thr Glu Lys Arg Gln Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Met His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn Glu 
145                 150                 155                 160 


Ala Pro Val Ala Ser Phe Lys Ser Ser Glu Ile Asn Leu Ala Glu His 
                165                 170                 175     


Phe Asp Gln Leu Asn Thr Ile Phe Ala Asp Leu Phe Arg Glu Ser Gly 
            180                 185                 190         


Phe Gln Leu Glu Thr Asp Glu Leu Val Ala Val Lys Ala Leu Leu Met 
        195                 200                 205             


Asp Asn Gln Gln Ser Ala Ser Asn Arg Gln Arg Lys Ala Leu Pro Lys 
    210                 215                 220                 


Leu Tyr Thr Pro Ser Ala Asp Lys Ser Ile Glu Lys Gln Arg Lys Ala 
225                 230                 235                 240 


Ile Ala Thr Glu Ile Leu Lys Ala Ile Leu Gly Leu Lys Ala Lys Phe 
                245                 250                 255     


Asn Val Val Thr Gly Ile Glu Ala Glu Asp Val Lys Ala Trp Ala Leu 
            260                 265                 270         


Thr Phe Asn Ala Glu Asn Phe Asp Glu Glu Leu Ala Lys Leu Glu Ser 
        275                 280                 285             


Ser Leu Asp Asp Asn Gly His Glu Val Ile Gly Ile Leu Gln Glu Leu 
    290                 295                 300                 


Tyr Ser Gly Ile Leu Leu Ala Gly Ile Val Pro Glu Asn Gln Ser Leu 
305                 310                 315                 320 


Ser Gln Ala Met Ile Thr Lys Tyr Glu Glu His Gln Ala His Leu Lys 
                325                 330                 335     


Met Leu Lys Ala Val Arg Asp Asp Leu Ala Thr Ala Asp Gln Gln Lys 
            340                 345                 350         


Leu Lys Lys Ala Tyr Asp Gln Tyr Ile Asp Gly Gln Glu Asn Thr Lys 
        355                 360                 365             


Ala Tyr Ser Lys Glu Ala Phe Tyr Asp Asp Val Thr Lys Ala Leu Lys 
    370                 375                 380                 


Asp Tyr Ser Glu Gln Pro Leu Val Ser Glu Ile Gln Lys Leu Ile Glu 
385                 390                 395                 400 


Leu Asp Gln Phe Met Pro Lys Gln Arg Thr Lys Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Gln Gln Glu Leu Asp Arg Ile Ile Glu Asn Gln 
            420                 425                 430         


Gln Gln Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Pro Lys Arg 
        435                 440                 445             


Gln Thr Val Ala Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ile Thr Ala Glu Gln Gln Gln Gln Ser 
465                 470                 475                 480 


Ser Asn Ala Lys Phe Ala Trp Met Ile Arg Lys Thr Glu Gly Arg Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Asp Lys Val Asp Arg Gln Ala Ser Ala Asn 
            500                 505                 510         


Glu Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu Ala Glu 
        515                 520                 525             


Asp Val Leu Pro Lys Gln Ser Leu Ile Tyr Gln Arg Phe Glu Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Gly Leu Lys Ile Asp Asp Gln Pro Ile Thr Asn Glu 
545                 550                 555                 560 


Leu Lys Gln Ala Ile Phe Asn Asp Leu Phe Met Gln Lys Thr Ser Val 
                565                 570                 575     


Ser Val Lys Asn Ile Gln Asp Tyr Leu Val Ser Glu Lys Arg Tyr Ala 
            580                 585                 590         


Ser Arg Pro Ala Ile Thr Gly Leu Ser Asp Glu Lys Lys Phe Asn Ser 
        595                 600                 605             


Arg Leu Ser Thr Tyr His Asp Leu Lys Val Ile Val Gly Asp Ala Val 
    610                 615                 620                 


Asp Asp Val Asp Lys Gln Ala Asp Leu Glu Lys Cys Ile Glu Trp Ser 
625                 630                 635                 640 


Thr Ile Phe Glu Asp Gly Lys Ile Tyr Ser Ala Lys Leu Asn Glu Ile 
                645                 650                 655     


Thr Trp Leu Thr Glu Gln Gln Arg Gly Gln Leu Ala Thr Lys Arg Tyr 
            660                 665                 670         


Arg Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Thr Gln Ile Val Asn 
        675                 680                 685             


Ala Asn Gly Gln Arg Ile Met Asp Leu Leu Trp Asp Thr Thr Asp Asn 
    690                 695                 700                 


Phe Met Arg Ile Val His Phe Glu Asp Phe Asp Lys Leu Ile Thr Glu 
705                 710                 715                 720 


Ala Asn Gln Met Val Leu Ala Glu Asn Asp Val Gln Asp Val Ile Asn 
                725                 730                 735     


Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Ile Leu 
            740                 745                 750         


Leu Val Val Asn Asp Ile Gln Lys Ala Met Lys Gly Gln Ala Pro Glu 
        755                 760                 765             


Arg Ile Leu Ile Glu Phe Ala Arg Gly Ala Gln Lys Asp Lys Ser Leu 
    770                 775                 780                 


Thr Val Arg Arg Lys Arg Lys Leu Asp Glu Met Tyr Ser Gln Ile Ser 
785                 790                 795                 800 


Asp Ala Val Leu Ala Asp Glu Ser Val Arg Gln Glu Leu Lys Ser Ser 
                805                 810                 815     


Asn Gln Asp Asp Leu Val Lys Glu Lys Val Tyr Leu Tyr Phe Met Gln 
            820                 825                 830         


Gly Gly Arg Asp Leu Tyr Thr Gly Asn Ala Leu Asn Ile Asp Arg Leu 
        835                 840                 845             


Ser Asp Tyr His Val Asp His Ile Ile Pro Gln Ala Tyr Ile Lys Asp 
    850                 855                 860                 


Asp Ser Leu Asp Asn Lys Ala Leu Val Ser Ser Arg Met Asn Glu Ile 
865                 870                 875                 880 


Lys Ala Asp Arg Val Pro Leu Gln Val Val Gln Lys Gln Phe Gly Lys 
                885                 890                 895     


Asn Met Ala Ser Glu Trp Gly Tyr Met His Arg Ala Gly Leu Ile Ser 
            900                 905                 910         


Arg Lys Lys Tyr Asp Asn Leu Met Phe Asn Pro Glu Asn Leu Asn Lys 
        915                 920                 925             


Tyr Gln Ile Asn Arg Phe Ile Ser Arg Gln Leu Val Glu Thr Gln Gln 
    930                 935                 940                 


Val Ile Lys Leu Val Ala Asn Leu Leu Ser Asn Gln Tyr Glu Gly Asp 
945                 950                 955                 960 


Asn Val Lys Ile Ile Thr Val Lys Ser Lys Leu Thr Gln Thr Ile Arg 
                965                 970                 975     


Ser Glu Phe Asp Phe Pro Lys Asn Arg Glu Val Asn Asn Tyr His His 
            980                 985                 990         


Ala Phe Asp Ala Tyr Leu Thr Ala  Phe Val Gly Met Tyr  Leu Leu Lys 
        995                 1000                 1005             


Arg Tyr  Pro Lys Leu Ser Ser  Tyr Phe Thr Tyr Gly  Glu Tyr Leu 
    1010                 1015                 1020             


Lys Gly  Asn Gln Gln Asp Lys  Trp Ser Ser Phe Asn  Phe Leu Asn 
    1025                 1030                 1035             


Asp Leu  Lys Arg Ala Lys Leu  Glu Asp Lys Thr Asn  Lys Glu Ile 
    1040                 1045                 1050             


Phe Trp  His Lys Glu Ser Gly  Leu Ala Tyr Leu Asn  Lys Ile Tyr 
    1055                 1060                 1065             


Gln Phe  Lys Lys Ile Leu Val  Thr Arg Glu Val His  Glu Asn Ser 
    1070                 1075                 1080             


Gly Ala  Leu Phe Asn Gln Thr  Leu Tyr Ala Ala Lys  Asp Asp Lys 
    1085                 1090                 1095             


Ala Ser  Gly Gln Gly Gly Lys  Gln Leu Ile Pro Ala  Lys Gln Ala 
    1100                 1105                 1110             


Arg Pro  Thr Ala Leu Tyr Gly  Gly Tyr Ser Gly Lys  Thr Val Ala 
    1115                 1120                 1125             


Tyr Met  Cys Ile Val Arg Ile  Lys Asn Lys Lys Gly  Asp Leu Tyr 
    1130                 1135                 1140             


Lys Val  Cys Gly Val Glu Thr  Ser Trp Leu Ala Gln  Leu Lys Gln 
    1145                 1150                 1155             


Leu Lys  Asp Glu Asp Ser Gln  Lys Ala Phe Leu Lys  Gln Lys Ile 
    1160                 1165                 1170             


Ser Pro  Gln Phe Thr Lys Ala  Lys Lys Gln Lys Gly  Thr Ile Val 
    1175                 1180                 1185             


Lys Val  Val Glu Asp Phe Glu  Val Ile Ala Pro His  Ile Leu Ile 
    1190                 1195                 1200             


Asn Gln  Arg Leu Phe Asp Asn  Gly Gln Glu Leu Thr  Leu Gly Ser 
    1205                 1210                 1215             


Ala Thr  Tyr Lys His Asn Glu  Gln Glu Leu Ile Leu  Asp Lys Thr 
    1220                 1225                 1230             


Ala Val  Lys Leu Leu Asn Gly  Glu Leu Pro Leu Ala  Gln Ser Glu 
    1235                 1240                 1245             


Glu Leu  Ala Glu Leu Val Tyr  Asp Glu Ile Leu Asp  Gln Val Met 
    1250                 1255                 1260             


His Tyr  Phe Pro Leu Tyr Asp  Thr Asn Gln Phe Arg  Ala Lys Leu 
    1265                 1270                 1275             


Leu Ala  Gly Lys Thr Ala Phe  Met Gln Leu Pro Trp  Lys Ser Asn 
    1280                 1285                 1290             


Pro Asp  Asp Asn Lys Thr Val  Gln Ile Thr Gln Pro  Ile Val Leu 
    1295                 1300                 1305             


Lys Lys  Ile Leu Gln Gly Leu  His Ala Asp Ala Gly  Arg Val Ser 
    1310                 1315                 1320             


Leu Lys  Glu Leu Gly Met Thr  Thr Asp Phe Gly Met  Leu Gln Lys 
    1325                 1330                 1335             


Ala Thr  Gly Ile Thr Phe Thr  Pro Asp Thr Gln Ile  Ile Tyr Gln 
    1340                 1345                 1350             


Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Val Ala Leu  Arg Asp Leu 
    1355                 1360                 1365             


<210>  994
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  994

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  995
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  995

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Leu 
            20                  25                  30          


Lys Gly Leu Gly Asn Thr Asp Arg His Gly Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Val Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Thr Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Asp Ile Leu Lys Glu Tyr Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Arg Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asp Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Arg Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  996
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  996

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Arg Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Glu Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Asp Ile Leu Lys Glu Tyr Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Lys Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Arg Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Phe Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Ile  Tyr Phe Asp Thr Thr  Ile Gly Arg Asn Arg  Tyr Lys Ser 
    1325                 1330                 1335             


Ile Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  997
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  997

Met Lys Tyr Glu Ser Glu Glu Tyr Tyr Ile Gly Leu Asp Met Gly Thr 
1               5                   10                  15      


Ser Ser Val Gly Trp Ala Val Thr Asp Pro His Tyr His Leu Ile Arg 
            20                  25                  30          


Lys Lys Gly Lys Asp Leu Trp Gly Ala Arg Leu Phe Glu Lys Ala Asn 
        35                  40                  45              


Thr Ala Gln Glu Arg Arg Thr Asn Arg Ile Asn Arg Arg Arg Asn Gln 
    50                  55                  60                  


Arg Arg Lys Gln Arg Ile Ala Phe Ile Arg Glu Ala Phe Arg Lys Glu 
65                  70                  75                  80  


Val Glu Lys Val Asp Val Asn Phe Phe Arg Arg Leu Asp Glu Ser Gln 
                85                  90                  95      


Leu Tyr Leu Glu Asp Lys Ser Ile Lys Glu Pro Tyr Leu Leu Phe Asn 
            100                 105                 110         


Asp Leu Asn Tyr Thr Asp Lys Asp Phe Tyr His Gln Tyr Pro Thr Val 
        115                 120                 125             


Phe His Leu Ile Thr Asp Leu Leu Thr Ser Asp Arg Lys Phe Asp Ile 
    130                 135                 140                 


Arg Glu Ile Tyr Ile Ala Val Ser Ser Leu Tyr Asn His Arg Gly Asn 
145                 150                 155                 160 


Phe Leu Asn Lys Ser Leu Gly Asn Glu Thr Gln Asp Glu Asp Leu Val 
                165                 170                 175     


Phe Lys Asn Leu Asp Glu Ala Phe Ser Ala Cys Gly Met Asn Trp Asn 
            180                 185                 190         


Thr Lys Ala Glu Asn Ile Glu Val Val Lys Gln Ile Leu Cys Asp Arg 
        195                 200                 205             


Ser Leu Asn Lys Lys Glu Lys Gln Asp Gln Leu Ala His Cys Leu Glu 
    210                 215                 220                 


Ile Asn Lys Lys Thr Glu Lys Glu Lys Ile Glu Phe Leu Lys Leu Leu 
225                 230                 235                 240 


Thr Gly Gly Lys Ala Asp Ser Lys Lys Met Phe Pro Gln Tyr His Tyr 
                245                 250                 255     


Glu Arg Asp Glu Lys Leu Ser Phe Ala Phe Ala Asp Glu Asn Gly Asp 
            260                 265                 270         


Glu Asn Trp Glu Glu Thr Leu Asn Ala Ile Glu Glu Arg Leu Thr Ser 
        275                 280                 285             


Asp Glu Tyr Gly Leu Ile Leu Ala Leu Lys Ser Val Tyr Asp Trp Ser 
    290                 295                 300                 


Ile Leu Ala Asp Leu Met Lys Asn Glu Lys Thr Gly Glu Thr Tyr Pro 
305                 310                 315                 320 


Tyr Leu Ser Met Ala Arg Lys Asp Ser Tyr Glu Lys His Lys Ala Asp 
                325                 330                 335     


Leu Arg Leu Leu Lys Ser Val Ile Lys Arg Leu Ile Pro Asn Glu Tyr 
            340                 345                 350         


Asn His Phe Phe Arg Gln Tyr Asp Gly Lys Gly Lys Thr Tyr Thr Asn 
        355                 360                 365             


Tyr Ile Gly Ser Val Gln Tyr His Glu Lys Arg His Arg Phe Lys His 
    370                 375                 380                 


Ile Thr Thr Lys Glu Phe Tyr Asp Gly Leu Lys Lys Leu Leu Lys Pro 
385                 390                 395                 400 


Tyr Ser Glu Asp Glu Glu Ile Lys Gln Ile Leu Gln Asp Ile Glu Ser 
                405                 410                 415     


Glu Thr Phe Leu Pro Lys Gln Ile Thr Asn Met Asn Gly Val Ile Pro 
            420                 425                 430         


Asn Gln Val His Ala Lys Glu Leu Lys Val Ile Leu Glu Lys Ala Ser 
        435                 440                 445             


Gln His Tyr Asp Phe Leu Asn Glu Thr Asn Glu Ser Gly Leu Thr Val 
    450                 455                 460                 


Lys Glu Gln Ile Met Gln Met Phe Thr Phe Gln Ile Pro Tyr Tyr Val 
465                 470                 475                 480 


Gly Pro Leu Ala Asn Leu Asn Gly Lys Gly Asn Gly Trp Ala Val Arg 
                485                 490                 495     


Tyr Val Gly Lys Glu Arg Thr Lys Val Tyr Pro Trp Asn Met Asp Glu 
            500                 505                 510         


Val Ile Asp Glu Lys Glu Thr Ala Asp Gln Phe Ile Lys Arg Met Ile 
        515                 520                 525             


Ser Thr Cys Thr Tyr Leu Gly Asp Glu Tyr Val Leu Pro Lys Ser Ser 
    530                 535                 540                 


Ile Leu Tyr Glu Lys Phe Ala Val Leu Asn Glu Leu Asn Ser Leu Lys 
545                 550                 555                 560 


Ile Asn Gly Gln Pro Val Ser Val Asp Leu Lys Gln Lys Ile Tyr Arg 
                565                 570                 575     


Asp Leu Phe Thr Lys Gly Lys Arg Val Thr Gln Lys Gly Leu Leu Leu 
            580                 585                 590         


Tyr Leu Lys Arg Glu Gly Val Ile Ser Lys Ser Glu Glu Thr Ile Leu 
        595                 600                 605             


Ser Gly Phe Asp Ile Asp Phe Lys Asn Thr Leu Ser Ser Tyr Arg Lys 
    610                 615                 620                 


Phe Cys Glu Val Phe Ala Thr Asn Glu Leu Asn Asp Glu Gln Ile Arg 
625                 630                 635                 640 


Met Ala Glu Asp Ile Ile Glu Trp Leu Thr Val Phe Gly Asp Ser Lys 
                645                 650                 655     


Lys Phe Val Arg Glu Lys Ile His Gly Asn Tyr Pro Gln Ile Thr Ala 
            660                 665                 670         


Asn Gln Leu Lys Arg Ile Leu Ser Phe Asn Phe Lys Asp Trp Gly Lys 
        675                 680                 685             


Leu Ser Lys Gln Phe Leu Glu Leu Glu Gly Glu Asp Arg Glu Thr Gly 
    690                 695                 700                 


Lys Ser Cys Ser Leu Ile Gln Ala Leu Trp Asp Thr Asn Asn Asn Leu 
705                 710                 715                 720 


Asn Gln Leu Leu Ser Ser Arg Phe Thr Tyr Lys Glu Asn Leu Glu Lys 
                725                 730                 735     


Lys Arg Lys Lys Ile Glu Met Asp Leu Phe Glu Phe Thr His Glu Asp 
            740                 745                 750         


Leu Asp Asp Leu Tyr Ile Ser Ala Pro Val Lys Arg Met Ile Trp Gln 
        755                 760                 765             


Ala Leu Lys Ile Met Lys Asp Ile Val Ile Val Met Lys Glu Ser Pro 
    770                 775                 780                 


Thr Lys Leu Phe Ile Glu Met Thr Arg Asn Asn Glu Asn Glu Lys Thr 
785                 790                 795                 800 


Arg Thr Val Ser Arg Lys Asn Lys Leu Val Asp Leu Tyr Lys Lys Ile 
                805                 810                 815     


Lys Gly Glu Ser Arg Asp Trp Ile Lys Glu Leu Glu Arg Tyr Glu Glu 
            820                 825                 830         


Lys Asp Phe Arg Ser Lys Lys Leu Tyr Leu Tyr Tyr Thr Gln Met Gly 
        835                 840                 845             


Lys Ser Ala Tyr Ser Gly Glu Pro Ile Asp Met Asp Lys Leu Phe Asp 
    850                 855                 860                 


Lys Asn Val Tyr Asp Ile Asp His Ile Tyr Pro Lys Ser Lys Val Lys 
865                 870                 875                 880 


Asp Asp Ser Leu Leu Asn Asn Cys Val Leu Val Lys Arg Lys Glu Asn 
                885                 890                 895     


Met Asp Lys Gly Asn Asn Phe Pro Ile Asp Pro Asp Ile Gln Thr Lys 
            900                 905                 910         


Gln Glu His Leu Trp Lys Phe Trp Leu Glu Lys Gly Leu Ile Thr Gln 
        915                 920                 925             


Glu Lys Phe Asn Arg Leu Thr Arg Lys Lys Pro Phe Thr Asn Asp Glu 
    930                 935                 940                 


Leu Ala Asp Phe Val Asn Arg Gln Leu Val Glu Thr Gly Gln Ala Thr 
945                 950                 955                 960 


Lys Thr Ile Ala Asp Leu Phe Lys Met Thr Phe Pro Asn Ser Asp Ile 
                965                 970                 975     


Val Tyr Val Lys Ala Gly Asn Val Thr Asp Phe Arg Gln Tyr Ile Glu 
            980                 985                 990         


Val Tyr Lys Cys Arg Glu Val Asn  Asn Leu His His Ala  Tyr Asp Ala 
        995                 1000                 1005             


Tyr Leu  Asn Val Val Val Gly  Asn Val Tyr Asp Thr  Lys Phe Thr 
    1010                 1015                 1020             


Lys Asn  Pro Met Asn Tyr Ile  Lys Glu Gln Arg Lys  Glu Asn Ser 
    1025                 1030                 1035             


Asn Glu  Arg Tyr His Met Asp  Lys Ile Phe Glu Phe  Pro Val Ile 
    1040                 1045                 1050             


Arg Asn  Gly Lys Glu Ala Trp  Ile Thr Lys Lys Asp  Glu Ser Ile 
    1055                 1060                 1065             


Asn Ile  Val Arg Lys Thr Leu  Ala Arg Lys Thr Pro  Leu Val Thr 
    1070                 1075                 1080             


Lys Arg  Asn Phe Glu Ala Asn  Gly Thr Ile Ala Lys  Ala Thr Ile 
    1085                 1090                 1095             


Tyr Pro  Lys Gly Asn Val Lys  Asp Gln Gly Tyr Leu  Pro Arg Lys 
    1100                 1105                 1110             


Thr Ser  Ile Pro Ala Leu Arg  Asp Met Lys Lys Tyr  Gly Gly Val 
    1115                 1120                 1125             


Thr Ser  Ile Gln Ile Ala Tyr  Phe Phe Leu Val Glu  His Glu Val 
    1130                 1135                 1140             


Lys Lys  Lys Arg Ile Arg Thr  Ile Glu Cys Val Pro  Ile Tyr Arg 
    1145                 1150                 1155             


Lys Glu  Glu Leu Lys Thr Lys  Glu Gln Leu Glu Arg  Tyr Cys Arg 
    1160                 1165                 1170             


Glu Glu  Leu Lys Leu Ile Asn  Pro Ser Ile Arg Leu  Glu Lys Ile 
    1175                 1180                 1185             


Lys Pro  Tyr Ser Leu Leu Lys  Met Asn Gly Asn Phe  Phe Tyr Ile 
    1190                 1195                 1200             


Thr Gly  Lys Thr Asn Asp Arg  Phe Ser Leu Ala Asn  Ala Met Glu 
    1205                 1210                 1215             


Leu Tyr  Val Asp Lys Val Ser  Glu Asn His Ile Arg  Asp Leu Val 
    1220                 1225                 1230             


Lys Tyr  Lys Asn Cys Gly Tyr  Phe Asn Val Asp Asp  Lys Glu Ala 
    1235                 1240                 1245             


Arg Lys  Arg Asn Glu Arg Leu  Tyr Thr Ile Leu Thr  Lys Lys His 
    1250                 1255                 1260             


Leu Thr  Val Phe Lys Gly Ser  Pro Lys Pro Met Gly  Glu Lys Leu 
    1265                 1270                 1275             


Gln Gln  Tyr Gln Gln Leu Phe  Leu Glu Leu Glu Val  Ser Lys Gln 
    1280                 1285                 1290             


Val Tyr  Val Leu Leu Gln Ile  Leu Gln Leu Thr Ala  Thr Asn Val 
    1295                 1300                 1305             


Lys Thr  Lys Ala Asp Leu Thr  Ala Ile Gly Gly Lys  Ala Asn Glu 
    1310                 1315                 1320             


Gly Thr  Met Asp Ile Ser Lys  Asn Leu Glu Asn Val  Ile Lys Asn 
    1325                 1330                 1335             


Gln Ile  Lys Lys Gly Leu Val  Pro Glu Leu Met Leu  Ile Asn Gln 
    1340                 1345                 1350             


Ser Pro  Thr Gly Leu Phe Glu  Ser Glu Ile Asp Leu  Leu Thr Ile 
    1355                 1360                 1365             


<210>  998
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  998

Met Ser Arg Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Trp Ser Val Val Asp Asp Gln Ser Lys Leu Val Ser Val Arg Gly 
            20                  25                  30          


Lys Tyr Gly Tyr Gly Val Arg Leu Tyr Asp Glu Gly Gln Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Gly Leu Leu Arg Glu Ile Phe Glu Pro Tyr Ile Thr Pro 
65                  70                  75                  80  


Val Asp Asp Thr Phe Phe Leu Arg Lys Lys Gln Ser Asn Leu Ser Pro 
                85                  90                  95      


Lys Asp Gln Arg Lys Leu Tyr Pro Gln Thr Ser Leu Phe Asn Asp Arg 
            100                 105                 110         


Thr Asp Arg Ala Phe Tyr Asp Asp Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Tyr Lys Leu Met Thr Glu Lys Arg Gln Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Met His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn Glu 
145                 150                 155                 160 


Ala Pro Val Ser Ser Phe Lys Ser Ser Glu Ile Asn Leu Val Ala His 
                165                 170                 175     


Phe Asp Arg Leu Asn Thr Ile Phe Ala Asp Leu Phe Ser Glu Ser Gly 
            180                 185                 190         


Phe Gln Leu Glu Thr Asp Lys Leu Ala Glu Val Lys Ala Leu Leu Leu 
        195                 200                 205             


Asp Asn His Gln Ser Ala Ser Asn Arg Gln Arg Gln Ala Leu Leu Leu 
    210                 215                 220                 


Ile Tyr Thr Pro Ser Thr Asn Lys Ala Val Glu Lys Gln Asn Lys Ala 
225                 230                 235                 240 


Ile Ala Thr Glu Leu Leu Lys Ala Ile Leu Gly Leu Lys Ala Lys Phe 
                245                 250                 255     


Asn Val Leu Thr Gly Ile Glu Ala Glu Asp Val Lys Thr Trp Thr Leu 
            260                 265                 270         


Thr Phe Asn Ala Glu Asn Phe Asp Glu Glu Met Val Lys Leu Glu Ser 
        275                 280                 285             


Ser Leu Asp Asp Asn Ala His Gln Ile Ile Glu Ser Leu Gln Glu Leu 
    290                 295                 300                 


Tyr Ser Gly Val Leu Leu Ala Gly Ile Val Pro Glu Asn Gln Ser Leu 
305                 310                 315                 320 


Ser Gln Ala Met Ile Thr Lys Tyr Asp Asp His Gln Lys His Leu Lys 
                325                 330                 335     


Met Leu Lys Ala Val Arg Glu Ala Leu Ala Pro Glu Asp Arg Gln Arg 
            340                 345                 350         


Leu Lys Gln Ala Tyr Asp Gln Tyr Val Asp Gly Gln Glu Asn Thr Lys 
        355                 360                 365             


Ala Tyr Ser Lys Glu Asp Phe Tyr Gly Asp Ile Thr Lys Ala Leu Lys 
    370                 375                 380                 


Asn Asn Pro Asp His Pro Ile Val Ser Glu Ile Lys Lys Leu Ile Glu 
385                 390                 395                 400 


Leu Asp Gln Phe Met Pro Lys Gln Arg Thr Lys Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Gln Gln Glu Leu Asp Arg Ile Ile Glu Asn Gln 
            420                 425                 430         


Gln Gln Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Ser Lys Arg 
        435                 440                 445             


Gln Thr Val Ala Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ile Thr Ala Glu Gln Gln Gln Gln Ser 
465                 470                 475                 480 


Ser Asp Ala Lys Phe Ala Trp Met Ile Arg Lys Ala Glu Gly Gln Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Asp Lys Val Asp Arg Gln Ala Ser Ala Asn 
            500                 505                 510         


Glu Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu Ala Glu 
        515                 520                 525             


Asp Val Leu Pro Lys Gln Ser Leu Ile Tyr Gln Arg Phe Glu Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Gly Leu Lys Ile Asp Asp Gln Pro Ile Thr Thr Glu 
545                 550                 555                 560 


Leu Lys Gln Ala Ile Phe Thr Asp Leu Phe Met Gln Lys Thr Ser Val 
                565                 570                 575     


Thr Val Lys Asn Ile Gln Asp Tyr Leu Val Ser Glu Lys Arg Tyr Ala 
            580                 585                 590         


Ser Arg Pro Ala Ile Thr Gly Leu Ser Asp Glu Asn Lys Phe Asn Ser 
        595                 600                 605             


Arg Leu Ser Thr Tyr His Asp Leu Lys Thr Ile Val Gly Asp Ala Val 
    610                 615                 620                 


Asp Asp Val Asp Lys Gln Ala Asp Leu Glu Lys Cys Ile Glu Trp Ser 
625                 630                 635                 640 


Thr Ile Phe Glu Asp Gly Lys Ile Tyr Ser Ala Lys Leu Asn Glu Ile 
                645                 650                 655     


Asp Trp Leu Thr Asp Gln Gln Arg Val Gln Leu Ala Ala Lys Arg Tyr 
            660                 665                 670         


Arg Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Thr Gln Ile Val Asn 
        675                 680                 685             


Ala Asn Gly Gln Arg Ile Met Asp Leu Leu Trp Asp Thr Thr Asp Asn 
    690                 695                 700                 


Phe Met Arg Ile Val His Ser Glu Asp Phe Asp Lys Leu Ile Thr Glu 
705                 710                 715                 720 


Ala Asn Gln Met Met Leu Ala Glu Asn Asp Val Gln Asp Val Ile Asn 
                725                 730                 735     


Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Ile Leu 
            740                 745                 750         


Leu Val Val Asn Asp Ile Gln Lys Ala Met Lys Gly Gln Ala Pro Glu 
        755                 760                 765             


Arg Ile Leu Ile Glu Phe Ala Arg Glu Asp Glu Val Asn Pro Arg Leu 
    770                 775                 780                 


Ser Val Gln Arg Lys Arg Gln Val Glu Gln Val Tyr Gln Asn Ile Ser 
785                 790                 795                 800 


Asn Glu Leu Leu Asn Asn Thr Glu Ile Arg Asn Glu Leu Lys Asp Leu 
                805                 810                 815     


Ser Asn Ser Ala Leu Ser Asn Thr Arg Leu Phe Leu Tyr Phe Met Gln 
            820                 825                 830         


Gly Gly Arg Asp Met Tyr Thr Gly Asp Ser Leu Asn Ile Asp Arg Leu 
        835                 840                 845             


Ser Thr Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe Ile Lys Asp 
    850                 855                 860                 


Asn Ser Leu Asp Asn Arg Val Leu Val Ser Gln Arg Met Asn Arg Ser 
865                 870                 875                 880 


Lys Ala Asp Gln Val Pro Thr Asp Phe Thr Ser Val Glu Leu Gly Gln 
                885                 890                 895     


Lys Met Gln Ile Gln Trp Glu Gln Met Leu Arg Ala Gly Leu Ile Thr 
            900                 905                 910         


Lys Lys Lys Tyr Asp Asn Leu Thr Leu Asn Pro Asp His Ile Ser Lys 
        915                 920                 925             


Tyr Ala Met Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Val Ile Lys Leu Ala Thr Asn Leu Leu Met Glu Gln Tyr Gly Glu Asp 
945                 950                 955                 960 


Asn Ile Glu Leu Ile Thr Val Lys Ser Gly Leu Thr His Gln Met Arg 
                965                 970                 975     


Thr Glu Phe Asp Phe Pro Lys Asn Arg Asn Leu Asn Asn His His His 
            980                 985                 990         


Ala Phe Asp Ala Tyr Leu Thr Ala  Phe Val Gly Leu Tyr  Leu Leu Lys 
        995                 1000                 1005             


Arg Tyr  Pro Lys Leu Lys Pro  Tyr Phe Val Tyr Gly  Glu Tyr Gln 
    1010                 1015                 1020             


Lys Ala  Ser Gln Gln Asp Lys  Trp Arg Asn Phe Asn  Phe Leu Asn 
    1025                 1030                 1035             


Gly Leu  Lys Lys Asp Glu Leu  Val Asp Glu Asn Thr  Glu Ala Val 
    1040                 1045                 1050             


Ile Trp  Asn Lys Glu Ser Gly  Leu Ala Tyr Leu Asn  Lys Ile Tyr 
    1055                 1060                 1065             


Gln Phe  Lys Lys Ile Leu Val  Thr Arg Glu Val His  Glu Asn Ser 
    1070                 1075                 1080             


Gly Ala  Leu Phe Asn Gln Thr  Leu Tyr Ala Ala Lys  Asp Asp Lys 
    1085                 1090                 1095             


Ala Ser  Gly Gln Gly Gly Lys  Gln Leu Ile Pro Ala  Lys Gln Asp 
    1100                 1105                 1110             


Arg Pro  Thr Ala Leu Tyr Gly  Gly Tyr Ser Gly Lys  Thr Val Ala 
    1115                 1120                 1125             


Tyr Met  Cys Ile Val Arg Ile  Lys Asn Lys Lys Gly  Asp Leu Tyr 
    1130                 1135                 1140             


Lys Val  Cys Gly Val Glu Thr  Ser Trp Leu Ala Gln  Leu Lys Gln 
    1145                 1150                 1155             


Leu Thr  Asp Glu Asp Ser Lys  Lys Ala Phe Leu Lys  Gln Lys Ile 
    1160                 1165                 1170             


Ser Pro  Gln Phe Thr Lys Val  Lys Lys Gln Lys Gly  Thr Ile Val 
    1175                 1180                 1185             


Lys Val  Val Glu Asp Phe Glu  Val Ile Ala Pro His  Ile Leu Ile 
    1190                 1195                 1200             


Asn Gln  Arg Phe Phe Asp Asn  Gly Gln Glu Leu Thr  Leu Gly Ser 
    1205                 1210                 1215             


Ala Thr  Tyr Lys His Asn Glu  Gln Glu Leu Ile Leu  Asp Lys Thr 
    1220                 1225                 1230             


Ala Val  Lys Leu Leu Asn Gly  Ala Leu Pro Leu Thr  Gln Ser Glu 
    1235                 1240                 1245             


Glu Leu  Ala Glu Gln Val Tyr  Asp Glu Ile Leu Asp  Gln Val Met 
    1250                 1255                 1260             


His Tyr  Phe Pro Leu Tyr Asp  Thr Asn Gln Phe Arg  Ala Lys Leu 
    1265                 1270                 1275             


Ser Ala  Gly Lys Ala Ala Phe  Met Lys Leu Pro Trp  Lys Ser Gln 
    1280                 1285                 1290             


Trp Asp  Gly Asn Lys Met Val  Gln Val Gly Gln Gln  Val Ile Leu 
    1295                 1300                 1305             


Asp Arg  Val Leu Ile Gly Leu  His Ala Asn Ala Ala  Val Ser Asp 
    1310                 1315                 1320             


Leu Gly  Val Leu Lys Ile Ser  Thr Pro Leu Gly Lys  Met Gln Gln 
    1325                 1330                 1335             


Pro Ser  Gly Ile Ser Leu Ser  Pro Asp Thr Gln Ile  Ile Tyr Gln 
    1340                 1345                 1350             


Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Val Ala Leu  Arg Asp Leu 
    1355                 1360                 1365             


<210>  999
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  999

Met Ser Arg Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Trp Ser Val Val Asp Asp Gln Ser Lys Leu Val Ser Val Arg Gly 
            20                  25                  30          


Lys Tyr Gly Tyr Gly Val Arg Leu Tyr Asp Glu Gly Gln Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Gly Leu Leu Arg Glu Ile Phe Glu Pro Tyr Ile Thr Pro 
65                  70                  75                  80  


Val Asp Asp Thr Phe Phe Leu Arg Gln Lys Gln Ser Asn Leu Ser Pro 
                85                  90                  95      


Lys Asp Gln Arg Lys Leu Tyr Pro Gln Thr Ser Leu Phe Asn Asp Arg 
            100                 105                 110         


Thr Asp Arg Ala Phe Tyr Asp Asp Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Tyr Lys Leu Met Thr Glu Lys Arg Gln Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Met His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn Glu 
145                 150                 155                 160 


Ala Pro Val Ser Ser Phe Lys Ser Ser Glu Ile Asn Leu Val Ala His 
                165                 170                 175     


Phe Asp Arg Leu Asn Thr Ile Phe Ala Asp Leu Phe Ser Glu Ser Gly 
            180                 185                 190         


Phe Gln Leu Glu Thr Asp Lys Leu Ala Glu Val Lys Ala Leu Leu Leu 
        195                 200                 205             


Asp Asn Gln Gln Ser Ala Ser Asn Arg Gln Arg Gln Ala Leu Ser Leu 
    210                 215                 220                 


Ile Tyr Thr Pro Ser Thr Asn Lys Ala Val Glu Lys Gln Asn Lys Ala 
225                 230                 235                 240 


Ile Ala Thr Glu Leu Leu Lys Ala Ile Leu Gly Leu Lys Ala Lys Phe 
                245                 250                 255     


Asn Val Leu Thr Arg Ile Glu Ala Glu Asp Val Lys Ala Trp Thr Leu 
            260                 265                 270         


Thr Phe Asn Ala Glu Asn Phe Asp Glu Glu Met Val Lys Leu Glu Ser 
        275                 280                 285             


Ser Leu Asp Asp Asn Ala His Gln Ile Ile Glu Ser Leu Gln Glu Leu 
    290                 295                 300                 


Tyr Ser Gly Ile Leu Leu Ala Gly Ile Val Pro Glu Asn Gln Ser Leu 
305                 310                 315                 320 


Ser Gln Ala Met Ile Met Lys Tyr Asp Asp His Gln Lys His Leu Lys 
                325                 330                 335     


Met Leu Lys Ala Val Arg Glu Ala Leu Ala Pro Glu Asp Arg Gln Arg 
            340                 345                 350         


Leu Lys Gln Ala Tyr Asp Gln Tyr Val Asp Gly Gln Glu Asn Thr Lys 
        355                 360                 365             


Ala Tyr Ser Lys Glu Asp Phe Tyr Gly Asp Ile Thr Lys Ala Leu Lys 
    370                 375                 380                 


Asn Asn Pro Asp His Pro Ile Val Ser Glu Ile Lys Lys Leu Ile Glu 
385                 390                 395                 400 


Leu Asp Gln Phe Met Pro Lys Gln Arg Thr Lys Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Gln Gln Glu Leu Asp Arg Ile Ile Glu Asn Gln 
            420                 425                 430         


Gln Gln Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Ser Lys Arg 
        435                 440                 445             


Gln Thr Val Ala Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ile Thr Ala Glu Gln Gln Arg Gln Ser 
465                 470                 475                 480 


Ser Asp Ala Lys Phe Ala Trp Leu Ile Arg Lys Ala Glu Gly Arg Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Asp Lys Val Asp Arg Gln Ala Ser Ala Asn 
            500                 505                 510         


Glu Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu Ala Glu 
        515                 520                 525             


Asp Val Leu Pro Lys Gln Ser Leu Ile Tyr Gln Arg Phe Glu Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Gly Leu Lys Ile Asp Asp Gln Pro Ile Thr Thr Glu 
545                 550                 555                 560 


Leu Lys Gln Ala Ile Phe Thr Asp Leu Phe Met Gln Lys Thr Ser Val 
                565                 570                 575     


Ser Val Lys Asn Ile Gln Asp Tyr Leu Val Ser Glu Lys Arg Tyr Ala 
            580                 585                 590         


Ser Arg Pro Ala Ile Thr Gly Leu Ser Asp Glu Asn Lys Phe Asn Ser 
        595                 600                 605             


Arg Leu Ser Thr Tyr His Asp Leu Lys Ala Ile Val Gly Asp Ala Val 
    610                 615                 620                 


Glu Asp Val Asp Lys Gln Val Asp Leu Glu Lys Cys Val Glu Trp Ser 
625                 630                 635                 640 


Thr Ile Phe Glu Asp Gly Lys Ile Tyr Ser Ala Lys Leu Asn Glu Ile 
                645                 650                 655     


Asp Trp Leu Thr Asp Gln Gln Arg Val Gln Leu Ala Ala Lys Arg Tyr 
            660                 665                 670         


Arg Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Thr Gln Ile Val Asn 
        675                 680                 685             


Ala Asn Gly Gln Arg Ile Met Asp Leu Leu Trp Asp Thr Thr Asp Asn 
    690                 695                 700                 


Phe Met Arg Ile Val His Ser Glu Asp Phe Asp Lys Leu Ile Thr Glu 
705                 710                 715                 720 


Ala Asn Gln Met Met Leu Ala Glu Asn Asp Val Gln Asp Val Ile Asn 
                725                 730                 735     


Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Ile Leu 
            740                 745                 750         


Leu Val Val Asn Asp Ile Gln Lys Ala Met Lys Gly Gln Ala Pro Glu 
        755                 760                 765             


Arg Ile Leu Ile Glu Phe Ala Arg Glu Asp Glu Val Asn Pro Arg Leu 
    770                 775                 780                 


Ser Val Gln Arg Lys Arg Gln Val Glu Gln Val Tyr Gln Asn Ile Ser 
785                 790                 795                 800 


Asn Glu Leu Leu Asn Asn Thr Glu Ile Arg Asn Glu Leu Lys Asp Leu 
                805                 810                 815     


Ser Asn Ser Ala Leu Ser Asn Thr Arg Leu Phe Leu Tyr Phe Met Gln 
            820                 825                 830         


Gly Gly Arg Asp Met Tyr Thr Gly Asp Ser Leu Asn Ile Asp Arg Leu 
        835                 840                 845             


Ser Thr Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe Ile Lys Asp 
    850                 855                 860                 


Asn Ser Leu Asp Asn Arg Val Leu Val Ser Gln Lys Met Asn Arg Ser 
865                 870                 875                 880 


Lys Ala Asp Gln Val Pro Thr Asp Phe Thr Ser Val Glu Leu Gly Lys 
                885                 890                 895     


Lys Met Gln Leu Gln Trp Glu Gln Met Leu Arg Ala Gly Leu Ile Thr 
            900                 905                 910         


Lys Lys Lys Tyr Asp Asn Leu Thr Leu Asn Pro Asp His Ile Ser Lys 
        915                 920                 925             


Tyr Ala Met Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Val Ile Lys Leu Ala Thr Asn Leu Leu Met Glu Gln Tyr Gly Glu Asp 
945                 950                 955                 960 


Asn Ile Glu Leu Ile Thr Val Lys Ser Gly Leu Thr His Gln Met Arg 
                965                 970                 975     


Thr Glu Phe Asp Phe Pro Lys Asn Arg Asn Leu Asn Asn His His His 
            980                 985                 990         


Ala Phe Asp Ala Tyr Leu Thr Ala  Phe Val Gly Leu Tyr  Leu Leu Lys 
        995                 1000                 1005             


Arg Tyr  Pro Lys Leu Lys Pro  Tyr Phe Val Tyr Gly  Glu Tyr Gln 
    1010                 1015                 1020             


Lys Ala  Ser Gln Gln Asp Lys  Trp Arg Asn Phe Asn  Phe Leu Asn 
    1025                 1030                 1035             


Gly Leu  Lys Lys Asp Glu Leu  Val Asp Glu Asn Thr  Glu Ala Val 
    1040                 1045                 1050             


Ile Trp  Asp Lys Glu Ser Gly  Leu Ala Tyr Leu Asn  Lys Ile Tyr 
    1055                 1060                 1065             


Gln Phe  Lys Lys Ile Leu Val  Thr Arg Glu Val His  Glu Asn Ser 
    1070                 1075                 1080             


Gly Ala  Leu Phe Asn Gln Thr  Leu Tyr Ala Ala Lys  Asp Asp Lys 
    1085                 1090                 1095             


Ala Ser  Gly Gln Gly Gly Lys  Gln Leu Ile Pro Ala  Lys Gln Asp 
    1100                 1105                 1110             


Arg Pro  Thr Ala Leu Tyr Gly  Gly Tyr Ser Gly Lys  Thr Val Ala 
    1115                 1120                 1125             


Tyr Met  Cys Ile Val Arg Ile  Lys Asn Lys Lys Gly  Asp Leu Tyr 
    1130                 1135                 1140             


Lys Val  Cys Gly Val Glu Thr  Ser Trp Leu Ala Gln  Leu Lys Gln 
    1145                 1150                 1155             


Leu Thr  Asp Glu Asp Ser Gln  Lys Ala Phe Leu Lys  Gln Lys Ile 
    1160                 1165                 1170             


Ser Pro  Gln Phe Thr Lys Val  Lys Lys Gln Lys Gly  Thr Ile Val 
    1175                 1180                 1185             


Lys Val  Val Glu Asp Phe Lys  Val Ile Ala Pro His  Ile Leu Ile 
    1190                 1195                 1200             


Asn Gln  Arg Phe Phe Asp Asn  Gly Gln Glu Leu Thr  Leu Gly Ser 
    1205                 1210                 1215             


Ala Thr  Tyr Lys His Asn Glu  Gln Glu Leu Ile Leu  Asp Lys Thr 
    1220                 1225                 1230             


Ala Val  Lys Leu Leu Asn Gly  Ala Leu Pro Leu Thr  Gln Ser Glu 
    1235                 1240                 1245             


Glu Leu  Ala Glu Gln Val Tyr  Asp Glu Ile Leu Asp  Gln Val Met 
    1250                 1255                 1260             


His Tyr  Phe Pro Leu Tyr Asp  Thr Asn Gln Phe Arg  Ala Lys Leu 
    1265                 1270                 1275             


Ser Ala  Gly Lys Ala Ala Phe  Met Lys Leu Pro Trp  Lys Ser Ile 
    1280                 1285                 1290             


Trp Asp  Gly Asn Lys Met Thr  Gln Ile Gly Gln Gln  Ile Ile Leu 
    1295                 1300                 1305             


Asp Arg  Ile Leu Ile Gly Leu  His Ala Asn Ala Ala  Val Ser Asp 
    1310                 1315                 1320             


Leu Gly  Val Leu Lys Ile Ser  Thr Pro Leu Gly Lys  Met Gln Gln 
    1325                 1330                 1335             


Pro Ser  Gly Ile Ser Leu Ser  Pro Asp Thr Gln Ile  Ile Tyr Gln 
    1340                 1345                 1350             


Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Val Ala Leu  Arg Asp Leu 
    1355                 1360                 1365             


<210>  1000
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1000

Met Val Glu Lys Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys 
            20                  25                  30          


Lys Met Lys Val Leu Gly Asn Thr Ser Arg Gln Ser Ile Lys Lys Asn 
        35                  40                  45              


Met Ile Gly Ala Leu Leu Phe Asp Glu Gly Gly Pro Ala Ala Ser Thr 
    50                  55                  60                  


Arg Val Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg 
65                  70                  75                  80  


Leu Cys Tyr Leu Arg Asp Ile Phe Glu Ser Glu Met His Thr Ile Asp 
                85                  90                  95      


Lys His Phe Phe Leu Arg Leu Glu Asp Ser Phe Leu His Lys Ser Asp 
            100                 105                 110         


Lys Arg Tyr Glu Ala His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys 
        115                 120                 125             


Ala Tyr His Asp Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Ala Leu 
    130                 135                 140                 


Ala Asp Asn Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu 
145                 150                 155                 160 


Ala His Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Ala Leu 
                165                 170                 175     


Ser Ala Asn Asn Thr Asp Val Gln Gln Leu Val His Ala Leu Val Asp 
            180                 185                 190         


Ala Tyr Asn Ile Met Phe Glu Glu Asp Gln Leu Asp Ile Glu Ala Ile 
        195                 200                 205             


Asp Val Lys Ala Ile Leu Thr Glu Lys Ile Ser Lys Thr Arg Arg Leu 
    210                 215                 220                 


Glu Asn Leu Ile Ser Asn Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe 
225                 230                 235                 240 


Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser 
                245                 250                 255     


His Phe Asn Leu Pro Glu Asp Ala Lys Leu Gln Leu Ala Lys Asp Thr 
            260                 265                 270         


Tyr Asp Glu Glu Leu Asn Asn Leu Leu Thr Gln Ile Gly Asp Glu Tyr 
        275                 280                 285             


Ala Asp Leu Phe Leu Ser Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu 
    290                 295                 300                 


Ser Asp Ile Leu Thr Val Asn Gly Asp Gly Thr Gln Ala Pro Leu Ser 
305                 310                 315                 320 


Ala Ser Leu Ile Lys Arg Tyr Glu Glu His Arg Gln Asp Leu Ala Leu 
                325                 330                 335     


Leu Lys Gln Met Phe Lys Glu Gln Leu Pro Asp Leu Tyr Arg Asp Val 
            340                 345                 350         


Phe Thr Asp Glu Asn Lys Asp Gly Tyr Ala Gly Tyr Ile Ser Gly Lys 
        355                 360                 365             


Thr Ser Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Glu Thr 
    370                 375                 380                 


Leu Asp Gly Ala Glu Asp Phe Leu Thr Lys Ile Asn Arg Glu Asp Phe 
385                 390                 395                 400 


Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile 
                405                 410                 415     


His Leu Gly Glu Leu Gln Ala Ile Leu Glu Arg Gln Gln Ala Tyr Tyr 
            420                 425                 430         


Pro Phe Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe 
        435                 440                 445             


Arg Ile Pro Tyr Tyr Ile Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe 
    450                 455                 460                 


Ala Trp Leu Thr Arg Thr Ser Asp Gln Lys Ile Thr Pro Trp Asn Phe 
465                 470                 475                 480 


Asp Glu Met Val Asp Gln Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg 
                485                 490                 495     


Met Thr Asn Phe Asp Glu Tyr Leu Pro Gln Glu Lys Val Leu Pro Lys 
            500                 505                 510         


His Ser Leu Thr Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys 
        515                 520                 525             


Val Lys Tyr Val Thr Glu Gly Met Thr Lys Pro Glu Phe Leu Ser Ala 
    530                 535                 540                 


Gly Gln Lys Glu Gln Ile Val Glu Leu Leu Phe Lys Lys Tyr Arg Lys 
545                 550                 555                 560 


Val Thr Val Lys Gln Leu Lys Glu Asp Phe Phe Ser Lys Ile Glu Cys 
                565                 570                 575     


Phe Asp Thr Val Asp Ile Ser Gly Val Glu Asp Lys Phe Asn Ala Ser 
            580                 585                 590         


Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe 
        595                 600                 605             


Leu Asp Asn Ser Glu Asn Glu Asn Ile Ile Glu Asp Ile Ile Leu Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Lys Glu Met Ile Ala Asn Arg Leu Ala Val 
625                 630                 635                 640 


Tyr Glu Asp Leu Phe Asp Gln Asn Val Leu Lys Gln Leu Lys Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Lys Gln Leu Ile Asn Gly Met 
            660                 665                 670         


Arg Asp Lys His Thr Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp 
        675                 680                 685             


Gly Phe Ile Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu 
    690                 695                 700                 


Ser Phe Lys Glu Glu Ile Lys Lys Ala Gln Glu Gly Gly Leu Lys Asp 
705                 710                 715                 720 


Ser Ile Asn Asp Gln Ile Arg Asp Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Thr Ile Asn Ile Val Asp Glu Ile Val Lys Ile 
            740                 745                 750         


Met Gly Lys Ala Pro Gln His Ile Val Val Glu Met Ala Arg Asp Val 
        755                 760                 765             


Gln Lys Thr Asp Ile Gly Val Lys Gln Ser Arg Glu Arg Met Lys Arg 
    770                 775                 780                 


Val Gln Glu Val Leu Lys Lys Leu Gly Ser Gln Leu Leu Lys Glu His 
785                 790                 795                 800 


Pro Val Glu Asn Phe Gln Leu Gln Asn Glu Arg Leu Tyr Leu Tyr Tyr 
                805                 810                 815     


Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Ser Ile Ser 
            820                 825                 830         


Asn Leu Ser His Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile 
        835                 840                 845             


Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Glu His Asn 
    850                 855                 860                 


Arg Gly Lys Thr Asp Asn Val Pro Ser Ile Glu Val Val Lys Arg Met 
865                 870                 875                 880 


Lys Pro Tyr Trp Gln Lys Leu Leu Asp Thr Lys Val Ile Ser Gln Arg 
                885                 890                 895     


Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Gln Glu Ser 
            900                 905                 910         


Asp Lys Ala Asn Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile 
        915                 920                 925             


Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg 
    930                 935                 940                 


Asp Glu Lys Asp Arg Pro Ile Arg Arg Val Lys Val Ile Thr Leu Lys 
945                 950                 955                 960 


Ser Lys Phe Val Ser Asp Phe Arg Gln Asp Phe Gly Phe Tyr Lys Leu 
                965                 970                 975     


Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala 
            980                 985                 990         


Val Val Gly Thr Ala Leu Leu Lys  Met Tyr Pro Lys Leu  Ala Ser Glu 
        995                 1000                 1005             


Phe Val  Tyr Gly Asp Tyr Gln  Lys Tyr Asp Leu Lys  Arg Met Val 
    1010                 1015                 1020             


Gly Lys  Ser Gly Lys Ala Ser  Gly His Ala Thr Ala  Lys Tyr Phe 
    1025                 1030                 1035             


Phe Tyr  Ser Asn Leu Met Asn  Phe Phe Lys Ser Glu  Val Lys Leu 
    1040                 1045                 1050             


Ala Asn  Gly Asn Ile Ile Lys  Arg Ser Pro Ile Glu  Val Asn Glu 
    1055                 1060                 1065             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Thr Lys Asp  Phe Gly Thr 
    1070                 1075                 1080             


Val Arg  Lys Val Leu Ser Ala  Pro Gln Val Asn Ile  Val Lys Lys 
    1085                 1090                 1095             


Thr Glu  Ile Gln Thr Gly Gly  Phe Ser Asn Glu Thr  Ile Leu Ser 
    1100                 1105                 1110             


Lys Gly  Lys Ser Ser Lys Leu  Ile Pro Arg Lys Asn  Lys Trp Arg 
    1115                 1120                 1125             


Asp Thr  Thr Lys Tyr Gly Gly  Phe Asn Thr Pro Thr  Val Ala Tyr 
    1130                 1135                 1140             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ala Lys Lys 
    1145                 1150                 1155             


Leu Lys  Pro Val Lys Glu Leu  Val Gly Ile Thr Ile  Met Glu Arg 
    1160                 1165                 1170             


Thr Lys  Phe Glu Ala Asn Pro  Ile Ala Phe Leu Glu  Ser Lys Gly 
    1175                 1180                 1185             


Tyr His  Asp Ile Gln Glu His  Leu Met Ile Thr Leu  Pro Lys Tyr 
    1190                 1195                 1200             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Arg Arg Leu  Leu Ala Ser 
    1205                 1210                 1215             


Ala Thr  Glu Leu Gln Lys Gly  Asn Glu Met Val Leu  Pro Gln His 
    1220                 1225                 1230             


Leu Val  Thr Phe Leu Tyr Arg  Val Ser Lys Arg Asp  Lys Gly Thr 
    1235                 1240                 1245             


Gln Ser  Glu Asn Met Glu Tyr  Ile Ser Asn His Lys  Glu Lys Phe 
    1250                 1255                 1260             


Ile Glu  Ile Phe His Tyr Ile  Ile Arg Tyr Ala Glu  Lys Asn Val 
    1265                 1270                 1275             


Ile Lys  Pro Lys Val Ile Glu  Arg Leu Asn Asp Thr  Phe Asn Gln 
    1280                 1285                 1290             


Lys Phe  Asn Asp Ser Asp Leu  Thr Glu Leu Ser Ile  Ser Phe Leu 
    1295                 1300                 1305             


Asn Leu  Phe Lys Phe Thr Ser  Phe Gly Ala Pro Glu  Lys Phe Thr 
    1310                 1315                 1320             


Phe Leu  Asn Ser Glu Ile Lys  Gln Asp Asp Val Arg  Tyr Arg Ser 
    1325                 1330                 1335             


Thr Lys  Glu Cys Leu Asn Ser  Thr Leu Ile His Gln  Ser Val Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Phe  Gly Gly Asp 
    1355                 1360                 1365             


<210>  1001
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1001

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Ile Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Arg Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Leu Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Asp Ile Leu Lys Glu Tyr Pro 
785                 790                 795                 800 


Val Glu Thr Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Lys Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Arg Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Ile Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Val Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asp Pro Val Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Arg Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Cys Phe Asp Thr Thr  Ile Gly Arg Asn Arg  Tyr Lys Ser 
    1325                 1330                 1335             


Ile Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  1002
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1002

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Phe Val Gly Val Asp Glu Asn Gly Trp Pro Leu Asn Ile Lys Gly 
            20                  25                  30          


Lys Arg Ala Ile Gly Ala His Leu Phe Arg Glu Gly Glu Ala Ala Ala 
        35                  40                  45              


Glu Thr Arg Gly Phe Arg Thr Thr Arg Arg Arg Tyr Asn Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Ile Phe Asp Glu Pro Ile Ser Arg 
65                  70                  75                  80  


Val Asp Pro Thr Phe Phe Ala Arg Met Lys Glu Ser Ser Val Ser Pro 
                85                  90                  95      


Lys Asp Pro Asn Tyr Asp Gly Leu Ala Lys Glu Ile Leu Ile Pro Arg 
            100                 105                 110         


Asp Lys Asp Gly Lys Ile Leu Tyr Pro Thr Ile Tyr His Leu Arg His 
        115                 120                 125             


Ala Leu Met Tyr Glu Asp His Gln Phe Asp Ile Arg Glu Ile Tyr Leu 
    130                 135                 140                 


Ala Ile His His Ile Val Lys Tyr Arg Gly His Phe Leu Leu Asp Gly 
145                 150                 155                 160 


Ala Ala Lys Asn Phe Glu Ser Ser Asn Leu Asp Leu Lys Ser Asp Ile 
                165                 170                 175     


His Glu Ile Asn Ala Ile Leu Val Gly Ile Phe Gly Asn Glu Gln Val 
            180                 185                 190         


Gln Phe Asn Asp Gln Tyr Val Glu Lys Leu Lys Glu Val Leu Leu Asp 
        195                 200                 205             


Ser Thr Gln Ser Arg Ser Asp Arg Gln Lys Ala Ala Ala Lys Leu Leu 
    210                 215                 220                 


Leu Pro Thr Asn Asp Leu Asp Lys Asp Thr Thr Lys Ile Leu Lys Thr 
225                 230                 235                 240 


Val Ser Thr Glu Ile Thr Lys Ala Ile Thr Gly Leu Lys Ala Lys Phe 
                245                 250                 255     


Phe Val Ile Thr Gly Asn Asp Ile Ala Lys Asp Gln Gln Lys Asp Trp 
            260                 265                 270         


Thr Phe Ser Ile Asp Thr Leu Asp Glu Lys Met Ser Val Ile Glu Pro 
        275                 280                 285             


Ser Phe Ser Glu Lys Asn Phe Ser Leu Ile Thr Ile Leu Gln Lys Leu 
    290                 295                 300                 


Tyr Ser Ser Val Thr Leu Ala Gly Ile Val Pro Asn Gly Leu Ser Leu 
305                 310                 315                 320 


Ser Glu Ala Met Ile Gln Lys Tyr Asp Arg His Ala Ser Asp Leu Val 
                325                 330                 335     


Leu Leu Lys Lys Tyr Trp Asp Thr Lys Pro Lys Val Leu Lys His Gln 
            340                 345                 350         


Leu Gln Asn Thr Tyr Ala Glu Tyr Ile Glu Ile His Gln Val Val Asp 
        355                 360                 365             


Glu Phe Tyr Ser Asp Leu Ser Lys Leu Ile Lys Asn Asp Asp Asp Thr 
    370                 375                 380                 


Leu Ala Gln Glu Ile Lys Ser Lys Ile Thr Ile Gly Asn Phe Leu Pro 
385                 390                 395                 400 


Lys Gln Arg Thr Thr Glu Asn Gly Val Ile Pro Tyr Gln Leu Gln Gln 
                405                 410                 415     


Arg Glu Leu Asp Thr Ile Ile Lys Asn Gln Gln Glu Tyr Tyr Pro Trp 
            420                 425                 430         


Leu Ala Glu Glu Asn Pro Val Ala Asp His Gln Ile Ala Phe Pro Tyr 
        435                 440                 445             


Lys Leu Asp Glu Leu Val Gly Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
    450                 455                 460                 


Met Ile Ala Lys Glu Asp Thr Ser Asn Ser Lys Asn Ser Lys Phe Ala 
465                 470                 475                 480 


Trp Met Ile Arg Lys Gln Ser Gly Glu Val Thr Pro Trp Asn Phe Asp 
                485                 490                 495     


Glu Lys Ile Asp Arg Thr Ala Ser Ala Thr Ala Phe Ile Asp Arg Met 
            500                 505                 510         


Thr Thr Thr Asp Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Gln 
        515                 520                 525             


Ser Leu Ile Tyr Gln Arg Phe Glu Val Leu Asn Glu Leu Asn Lys Ile 
    530                 535                 540                 


Ser Ile Asp Gly Gln Pro Ile Thr Arg Lys Gln Lys Gln Ser Val Tyr 
545                 550                 555                 560 


Thr His Val Phe Glu Lys Lys Lys Thr Val Gln Val Lys Asp Val Gln 
                565                 570                 575     


Lys Phe Leu Val Ser Ser Gly Glu Val Met Ser Leu Pro Lys Ile Gly 
            580                 585                 590         


Gly Leu Ala Asn Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr Leu Tyr 
        595                 600                 605             


Asp Tyr Asn Lys Ile Ile Pro Asp Ala Leu Glu Asp Arg Asn Lys Val 
    610                 615                 620                 


Asp Asp Ile Glu Lys Ile Ile Leu Trp Ser Thr Ile Phe Glu Asp Asn 
625                 630                 635                 640 


Ser Ile Phe Val Glu Lys Leu Asp Ser Ile Asp Trp Leu Thr Ser Ser 
                645                 650                 655     


Gln Arg Lys Arg Leu Ala Gln Lys Arg Tyr Arg Gly Trp Gly Arg Leu 
            660                 665                 670         


Ser Leu Arg Leu Leu Thr Thr Phe Lys Asn Lys Asp Gly Tyr Ser Ile 
        675                 680                 685             


Leu Asp Glu Leu Trp Asn Ser Asn His Asn Phe Met Glu Ile Gln Ala 
    690                 695                 700                 


Gln Ser Asp Phe Ser Glu Gln Ile Ala Thr Ala Asn Asn Asn Asn Leu 
705                 710                 715                 720 


Ser Gln Val Leu Pro Ala Lys Trp Glu Pro Gly Arg Lys Asn Leu Ala 
                725                 730                 735     


Ser Leu Asp Glu Phe Ile Ser Asn Leu Tyr Thr Ser Pro Gln Asn Lys 
            740                 745                 750         


Lys Ala Leu Arg Lys Ala Ile Arg Val Val Gln Asp Met Met Thr Ala 
        755                 760                 765             


Ala Gly His Met Pro Glu Lys Ile Phe Ile Glu Val Ala Arg Asp Thr 
    770                 775                 780                 


Ser Asn Ser His Gln Gln Thr Ile Ala Arg Gln Arg Gln Leu Gln Gln 
785                 790                 795                 800 


Leu Tyr Gln Ser Glu Asp Lys Glu Val Ile Asp Ser Ser Val Arg Ser 
                805                 810                 815     


Glu Leu Asp Asp Ile Leu Lys Asn Arg Ala Asn Ile Lys Asp Gln Leu 
            820                 825                 830         


Phe Leu Tyr Phe Gln Gln Gly Gly Arg Asp Leu Tyr Thr Gly Lys Ala 
        835                 840                 845             


Ile Asn Ile Asp Lys Leu Ser Leu Tyr His Ile Asp His Ile Leu Pro 
    850                 855                 860                 


Gln Ser Leu Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser 
865                 870                 875                 880 


Gln Lys Thr Asn Glu Asp Lys Gly Val Thr Phe Val Ser Asp Lys Tyr 
                885                 890                 895     


Met Asn Asn Met Leu Pro Thr Trp Lys Arg Trp His Glu Met Gly Leu 
            900                 905                 910         


Ile Ser Ala Arg Lys Leu Lys Asn Leu Thr Met Arg Pro Asp Glu Ile 
        915                 920                 925             


Asp Lys Tyr Ala Pro Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg 
    930                 935                 940                 


Gln Val Thr Lys Leu Val Ile Glu Leu Leu Asp Ala Leu Leu Gly Asn 
945                 950                 955                 960 


Ser Gln Thr Glu Ile Ile Thr Ile Lys Ala Ser Leu Ser His Gln Phe 
                965                 970                 975     


Arg Gln Gln Phe Asp Leu Val Lys Ile Arg Glu Val Asn Asp Asn His 
            980                 985                 990         


His Ala Phe Asp Ala Phe Leu Ala  Ala Arg Ile Gly Thr  Tyr Leu Tyr 
        995                 1000                 1005             


Lys Arg  Tyr Pro Lys Leu Arg  Pro Tyr Phe Val Tyr  Gly Gln Tyr 
    1010                 1015                 1020             


Met Gln  Asn His Lys Asn Leu  Lys Ser Ile Asn Leu  Ile Lys Thr 
    1025                 1030                 1035             


Leu Asp  Ser Asp Lys Ala Ile  Val Asp Glu Gly Thr  Gly Glu Ile 
    1040                 1045                 1050             


Leu Trp  Asp Lys Lys Ser Asp  Leu Ala Lys Met Ile  Lys Ile Tyr 
    1055                 1060                 1065             


Asn Tyr  Gln Arg Leu Leu Ile  Thr His Glu Ser Arg  Thr Ser Phe 
    1070                 1075                 1080             


Gly Ala  Met Tyr Lys Gln Lys  Ile Leu Lys Ala Thr  Asp Ala Lys 
    1085                 1090                 1095             


Gln Asn  Gly Gly Gly Phe Lys  Leu Val Pro Thr Lys  Arg Asn Arg 
    1100                 1105                 1110             


Pro Val  Asp Ile Tyr Gly Gly  Tyr Thr Gly Gln Thr  Ala Ala Tyr 
    1115                 1120                 1125             


Leu Thr  Ile Val Arg Val Tyr  Ser Lys Lys Thr Ser  Glu Leu Arg 
    1130                 1135                 1140             


Val Leu  Pro Ile Tyr Thr Asn  Lys Val Gln Gln Ile  Glu Lys Ala 
    1145                 1150                 1155             


Met Thr  Gln Gly Asp Lys Ala  Thr Met Leu Ala Phe  Lys Lys Val 
    1160                 1165                 1170             


Ile Ala  Pro Gln Leu Gly Lys  Glu Asn Lys Lys Thr  His Glu Ile 
    1175                 1180                 1185             


Lys Ile  Pro Glu Phe Asp Val  Val Ile Pro Lys Val  Leu Leu Asp 
    1190                 1195                 1200             


Gln Leu  Val Ile Asp Asp Ile  Ala Gly Ser Pro His  Arg Phe Arg 
    1205                 1210                 1215             


Leu Gly  Thr Asp Thr Tyr Tyr  His Asn Ser Gln Gln  Leu Phe Leu 
    1220                 1225                 1230             


Pro Leu  Ser Met Gln Arg Gln  Leu Met Asp Arg Glu  Ser Asp Asp 
    1235                 1240                 1245             


Lys Val  Tyr Leu Asp Ile Phe  Asn Asp Val Leu Lys  Gln Val Asp 
    1250                 1255                 1260             


Ser Tyr  Phe Asn Leu Tyr Asp  Val Asn Gly Phe Arg  Ala Ala Leu 
    1265                 1270                 1275             


Gln Lys  Ser Lys Lys Ala Phe  Glu Gln Leu Pro Ile  Asn Asp Phe 
    1280                 1285                 1290             


Pro Lys  Asp Val Pro Asp Asn  Lys Lys Lys Lys Gly  Lys Arg Ser 
    1295                 1300                 1305             


Ile Leu  Lys Glu Ile Phe Ile  Gly Leu His Ala Asn  Ala Thr Leu 
    1310                 1315                 1320             


Gly Asn  Leu Lys Gln Met Gly  Ile Lys Thr Asp Phe  Gly Lys Leu 
    1325                 1330                 1335             


Gln Lys  Ser Asn Gly Leu Arg  Ile Thr Glu Asn Cys  Lys Ile Ile 
    1340                 1345                 1350             


His Gln  Ser Pro Thr Gly Leu  Phe Glu Arg Ile Val  Pro Ile Lys 
    1355                 1360                 1365             


<210>  1003
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1003

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Ile Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Leu Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Val Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Leu Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Arg Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Thr Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Phe Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  1004
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1004

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Leu Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asn Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Arg Lys Asp Leu Ile  Val Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Phe Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  1005
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1005

Met Ser Arg Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Trp Ser Val Val Asp Asp Gln Ser Lys Leu Val Ser Val Arg Gly 
            20                  25                  30          


Lys Tyr Gly Tyr Gly Val Arg Leu Tyr Asp Glu Gly Gln Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Gly Leu Leu Arg Glu Ile Phe Glu Pro Tyr Ile Thr Pro 
65                  70                  75                  80  


Ile Asp Asp Thr Phe Phe Leu Arg Gln Lys Gln Ser Asn Leu Ser Pro 
                85                  90                  95      


Lys Asp Gln Arg Lys Leu Tyr Thr Gln Thr Ser Leu Phe Asn Asp Arg 
            100                 105                 110         


Thr Asp Arg Ala Phe Tyr Asp Asp Tyr Pro Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Tyr Arg Leu Met Thr Glu Lys Arg Gln Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Met His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn Glu 
145                 150                 155                 160 


Ala Pro Val Ser Ser Phe Lys Ser Ser Glu Ile Asn Leu Val Ala His 
                165                 170                 175     


Phe Asp Arg Leu Asn Thr Ile Phe Ala Asp Leu Phe Ser Glu Ser Gly 
            180                 185                 190         


Phe Gln Leu Glu Thr Asp Lys Leu Ala Glu Val Lys Ala Leu Leu Leu 
        195                 200                 205             


Asp Asn Gln Gln Ser Ala Ser Asn Arg Gln Arg Gln Ala Leu Ser Leu 
    210                 215                 220                 


Ile Tyr Thr Pro Ser Thr Asn Lys Ala Val Glu Lys Gln Asn Lys Ala 
225                 230                 235                 240 


Ile Ala Thr Glu Leu Leu Lys Ala Ile Leu Gly Leu Lys Ala Lys Phe 
                245                 250                 255     


Asn Val Leu Thr Gly Ile Glu Ala Glu Asp Val Lys Ala Trp Thr Leu 
            260                 265                 270         


Thr Phe Asn Ala Glu Asn Phe Asp Glu Glu Met Val Lys Leu Glu Ser 
        275                 280                 285             


Ser Leu Asp Asp Asn Ala His Gln Ile Ile Glu Ser Leu Gln Glu Leu 
    290                 295                 300                 


Tyr Ser Gly Val Leu Leu Ala Gly Ile Val Pro Glu Asn Gln Ser Leu 
305                 310                 315                 320 


Ser Gln Ala Met Ile Thr Lys Tyr Asp Asp His Gln Lys His Leu Lys 
                325                 330                 335     


Met Leu Lys Ala Val Arg Glu Ala Leu Ala Pro Gly Asp Arg Gln Arg 
            340                 345                 350         


Leu Lys Gln Ala Tyr Asp Gln Tyr Val Asp Gly Gln Glu Asn Thr Lys 
        355                 360                 365             


Ala Tyr Ser Lys Glu Asp Phe Tyr Gly Asp Ile Thr Lys Ala Leu Lys 
    370                 375                 380                 


Asn Asn Pro Asp His Pro Ile Val Ser Glu Ile Lys Lys Leu Ile Glu 
385                 390                 395                 400 


Leu Asp Gln Phe Met Pro Lys Gln Arg Thr Lys Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Gln Gln Glu Leu Asp Arg Ile Ile Glu Asn Gln 
            420                 425                 430         


Gln Gln Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Ser Lys Arg 
        435                 440                 445             


Gln Thr Val Ala Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ile Thr Ala Glu Gln Gln Arg Gln Ser 
465                 470                 475                 480 


Ser Asp Ala Lys Phe Ala Trp Leu Ile Arg Lys Ala Glu Gly Arg Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Asp Lys Val Asp Arg Gln Ala Ser Ala Asn 
            500                 505                 510         


Glu Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu Ala Glu 
        515                 520                 525             


Asp Val Leu Pro Lys Gln Ser Leu Ile Tyr Gln Arg Phe Glu Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Gly Leu Lys Ile Asp Asp Gln Pro Ile Thr Thr Glu 
545                 550                 555                 560 


Leu Lys Gln Ala Ile Phe Thr Asp Leu Phe Met Gln Lys Ile Ser Val 
                565                 570                 575     


Thr Val Lys Asn Ile Gln Asp Tyr Leu Val Ser Glu Lys Arg Tyr Ala 
            580                 585                 590         


Ser Arg Pro Ala Ile Thr Gly Leu Ser Asp Glu Asn Lys Phe Asn Ser 
        595                 600                 605             


Arg Leu Ser Thr Tyr His Asp Leu Lys Met Ile Val Gly Asp Ala Val 
    610                 615                 620                 


Asp Asp Val Asp Lys Gln Ala Asp Leu Glu Lys Cys Ile Glu Trp Ser 
625                 630                 635                 640 


Thr Ile Phe Glu Asp Gly Lys Ile Tyr Ser Ala Lys Leu Asn Glu Ile 
                645                 650                 655     


Asp Trp Leu Thr Asp Gln Gln Arg Val Gln Leu Ala Ala Lys Arg Tyr 
            660                 665                 670         


Arg Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Thr Gln Ile Val Asn 
        675                 680                 685             


Ala Asn Gly Gln Arg Ile Met Asp Leu Leu Trp Asp Thr Thr Asp Asn 
    690                 695                 700                 


Phe Met Arg Ile Val His Ser Glu Asp Phe Asp Lys Leu Ile Thr Glu 
705                 710                 715                 720 


Ala Asn Gln Met Met Leu Ala Glu Asn Asp Val Gln Asp Val Ile Asn 
                725                 730                 735     


Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Ile Leu 
            740                 745                 750         


Leu Val Val Asn Asp Ile Gln Lys Ala Met Lys Gly Gln Ala Pro Glu 
        755                 760                 765             


Arg Ile Leu Ile Glu Phe Ala Arg Glu Asp Glu Val Asn Ser Arg Leu 
    770                 775                 780                 


Ser Val Gln Arg Lys Arg Gln Val Glu Gln Val Tyr Gln Asn Ile Ser 
785                 790                 795                 800 


Asn Glu Leu Leu Asn Asn Thr Glu Ile Arg Asn Glu Leu Lys Asp Leu 
                805                 810                 815     


Ser Asn Ser Ala Leu Ser Asn Thr Arg Leu Phe Leu Tyr Phe Met Gln 
            820                 825                 830         


Gly Gly Arg Asp Met Tyr Thr Gly Asp Ser Leu Asn Ile Asp Arg Leu 
        835                 840                 845             


Ser Thr Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe Ile Lys Asp 
    850                 855                 860                 


Asn Ser Leu Asp Asn Arg Val Leu Val Ser Gln Lys Met Asn Arg Ser 
865                 870                 875                 880 


Lys Ala Asp Gln Val Pro Thr Asp Phe Thr Ser Val Glu Leu Gly Gln 
                885                 890                 895     


Lys Met Gln Ile Gln Trp Glu Gln Met Leu Arg Ala Gly Leu Ile Thr 
            900                 905                 910         


Lys Lys Lys Tyr Asp Asn Leu Thr Leu Asn Pro Asp His Ile Ser Lys 
        915                 920                 925             


Tyr Ala Met Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Val Ile Lys Leu Ala Thr Asn Leu Leu Met Glu Gln Tyr Gly Glu Asp 
945                 950                 955                 960 


Asn Ile Glu Leu Ile Thr Val Lys Ser Gly Leu Thr His Gln Met Arg 
                965                 970                 975     


Thr Glu Phe Asp Phe Pro Lys Asn Arg Asn Leu Asn Asn His His His 
            980                 985                 990         


Ala Phe Asp Ala Tyr Leu Thr Ala  Phe Val Gly Leu Tyr  Leu Leu Lys 
        995                 1000                 1005             


Arg Tyr  Pro Lys Leu Lys Pro  Tyr Phe Val Tyr Gly  Glu Tyr Gln 
    1010                 1015                 1020             


Lys Ala  Ser Gln Gln Asp Lys  Trp Arg Asn Phe Asn  Phe Leu Asn 
    1025                 1030                 1035             


Gly Leu  Lys Lys Asp Glu Leu  Val Asp Glu Asn Thr  Glu Ala Val 
    1040                 1045                 1050             


Ile Trp  Asp Lys Glu Ser Gly  Leu Ala Tyr Leu Asn  Lys Ile Tyr 
    1055                 1060                 1065             


Gln Phe  Lys Lys Ile Leu Val  Thr Arg Gly Val His  Glu Asn Ser 
    1070                 1075                 1080             


Gly Ala  Leu Phe Asn Gln Thr  Leu Tyr Ala Ala Lys  Asp Asp Lys 
    1085                 1090                 1095             


Ala Ser  Gly Gln Gly Gly Lys  Gln Leu Ile Pro Ala  Lys Gln Asp 
    1100                 1105                 1110             


Arg Ser  Thr Ala Leu Tyr Gly  Gly Tyr Ser Gly Lys  Thr Val Ala 
    1115                 1120                 1125             


Tyr Met  Cys Ile Val Arg Ile  Lys Asn Lys Lys Gly  Asp Leu Tyr 
    1130                 1135                 1140             


Lys Val  Cys Gly Val Glu Thr  Ser Trp Leu Ala Gln  Leu Lys Gln 
    1145                 1150                 1155             


Leu Thr  Asp Glu Asp Ser Gln  Lys Ala Phe Leu Glu  Gln Lys Ile 
    1160                 1165                 1170             


Ser Pro  Gln Phe Thr Lys Val  Lys Lys Gln Lys Gly  Asn Ile Ile 
    1175                 1180                 1185             


Glu Ala  Val Glu Glu Phe Glu  Val Ile Ala Pro His  Ile Leu Ile 
    1190                 1195                 1200             


Asn Gln  Arg Phe Phe Asp Asn  Gly Gln Glu Leu Thr  Leu Gly Ser 
    1205                 1210                 1215             


Ala Thr  Tyr Lys His Asn Glu  Gln Glu Leu Ile Leu  Asp Lys Thr 
    1220                 1225                 1230             


Ala Val  Lys Leu Leu Asn Gly  Ala Leu Pro Leu Thr  Gln Ser Glu 
    1235                 1240                 1245             


Glu Leu  Ala Glu Gln Val Tyr  Asp Glu Ile Leu Asp  Gln Val Met 
    1250                 1255                 1260             


His Tyr  Phe Pro Leu Tyr Asp  Thr Asn Gln Phe Arg  Ala Lys Leu 
    1265                 1270                 1275             


Ser Thr  Gly Lys Ala Ala Phe  Met Lys Leu Pro Trp  Lys Ser Gln 
    1280                 1285                 1290             


Trp Asp  Gly Asn Lys Met Val  Gln Val Gly Gln Gln  Val Ile Leu 
    1295                 1300                 1305             


Asp Arg  Ile Leu Ile Gly Leu  His Ala Asn Ala Ala  Met Ser Asp 
    1310                 1315                 1320             


Leu Lys  Val Leu Lys Met Thr  Thr Pro Phe Gly Met  Ile Gln Gln 
    1325                 1330                 1335             


Ser Thr  Gly Ile Thr Leu Ser  Ala Asp Thr Gln Ile  Ile Tyr Gln 
    1340                 1345                 1350             


Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Val Ala Leu  Arg Asp Leu 
    1355                 1360                 1365             


<210>  1006
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1006

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Leu Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Leu Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  1007
<211>  1369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1007

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Thr Val Asn Glu Lys Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Gln Lys Asn 
        35                  40                  45              


Phe Glu Ala Gly Lys Ser Ile Pro Thr Asn Ala Gly Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Asp Tyr 
65                  70                  75                  80  


Leu Ile Gln Cys Leu Lys Glu Tyr Gly Trp Ile Thr Ser Asp Thr Leu 
                85                  90                  95      


Leu Tyr Glu Cys Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Asp Glu Glu Ile Ala Leu Glu Glu Leu Gly Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Asn Arg Lys Ala 
    130                 135                 140                 


Lys Ser Ser Asp Glu Gly Gln Leu Ile Asp Gly Met Ala Ile Ala Met 
145                 150                 155                 160 


Asp Met Tyr His Asn Gly Thr Thr Pro Gly Glu Tyr Val Tyr Ser Leu 
                165                 170                 175     


Leu Ser Lys Gly Lys Lys Tyr Ile Pro Val Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Leu Asp Glu Phe Asp Arg Ile Trp Asp Tyr Gln Cys Ala Tyr Tyr Pro 
        195                 200                 205             


Asp Ile Leu Thr Ser Gln Phe Lys Glu Leu Ile Arg Gly Lys Ser Ser 
    210                 215                 220                 


Gln Val Thr Ser Lys Leu Phe Lys Gln Gln Tyr Asn Leu Phe Thr Ala 
225                 230                 235                 240 


Asp Asn Lys Glu Lys Asp Lys Arg Leu Gln Ala Tyr Cys Arg Arg Ala 
                245                 250                 255     


Asp Ala Leu Lys Lys Arg Leu Pro Val Glu Glu Val Ala Ala Ala Leu 
            260                 265                 270         


Cys Asp Val Asn Arg Asp Val Asn Ala Ser Ser Gly Tyr Leu Gly Thr 
        275                 280                 285             


Ile Ser Asp Arg Ser Lys Lys Leu Phe Phe Asn Lys Gln Thr Val Gly 
    290                 295                 300                 


Gln Tyr Leu Met Leu Glu Leu Asp Lys Asp Ser His Phe Ser Val Lys 
305                 310                 315                 320 


Asn Glu Val Phe Tyr Arg Gln Asp Tyr Leu Asn Glu Phe Glu Ala Ile 
                325                 330                 335     


Trp Glu Lys Gln Ala Glu Tyr His Pro Glu Leu Thr Pro Asp Arg Lys 
            340                 345                 350         


Lys Ile Leu Arg Asp Val Val Ile Phe Tyr Gln Arg Pro Leu Lys Ser 
        355                 360                 365             


Gln Lys Asp Leu Val Ala Cys Cys Glu Leu Glu Arg Arg Arg Ile Glu 
    370                 375                 380                 


Val Met Lys Asp Gly Lys Val Arg Ser Val Glu Ile Gly Pro Lys Val 
385                 390                 395                 400 


Cys Pro Lys Ser Ser Pro Leu Phe Gln Asp Phe Lys Ile Trp His Val 
                405                 410                 415     


Leu Asn Asn Leu Lys Val Arg Asp Lys Ala Thr Gly Asp Glu Arg Phe 
            420                 425                 430         


Leu Glu Leu Asp Glu Lys Glu Ile Leu Phe Ser Glu Leu Ala Ile Lys 
        435                 440                 445             


Ser Lys Leu Ser Lys Thr Glu Ala Leu Lys Leu Leu Tyr Gln Lys Pro 
    450                 455                 460                 


Lys Gly Leu Asp Leu Asn Tyr Asp Ser Val Glu Gly Asn Thr Thr Gln 
465                 470                 475                 480 


Ala Ala Leu Phe Glu Ala Tyr Gln Thr Val Ile Ala Met Thr Gly His 
                485                 490                 495     


Gly Glu Tyr Val Phe Ser Lys Met Ser Ala Ser Glu Val Met Ser Val 
            500                 505                 510         


Val Glu Gly Val Phe Gly Gly Leu Gly Phe Asn Thr Ser Val Leu Arg 
        515                 520                 525             


Phe Asp Ser Leu Leu Gln Gly Lys Glu Leu Glu Lys Gln Asp Ser Tyr 
    530                 535                 540                 


Arg Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asn Ser Val Thr 
545                 550                 555                 560 


Gly Thr Asp Gly Leu Val Ala Lys Val Ala Ser Leu Leu Arg Cys Asp 
                565                 570                 575     


Glu Glu Tyr Ala Lys Val Val Ala Gly Val Thr Phe Gln Pro Asp Tyr 
            580                 585                 590         


Gly Ser Leu Ser Ala Lys Ala Ile Arg Arg Ile Leu Pro His Met Arg 
        595                 600                 605             


Ala Gly Asn Glu Tyr Ser Leu Ala Cys Glu Tyr Ala Gly Tyr Lys His 
    610                 615                 620                 


Ser Arg Arg Ser Leu Thr Lys Glu Glu Leu Glu Gln Lys Asp Tyr Lys 
625                 630                 635                 640 


Asp His Leu Glu Leu Leu Pro Arg Asn Ser Leu Arg Asn Pro Val Val 
                645                 650                 655     


Glu Lys Ile Leu Asn Gln Met Val Asn Val Val Asn Ala Val Val Asp 
            660                 665                 670         


Ala Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Met Ala Arg Glu Leu 
        675                 680                 685             


Lys Lys Ser Ala Lys Glu Arg Glu Glu Met Thr Ala Ala Ile Asn Lys 
    690                 695                 700                 


Ala Thr Ser Glu His Glu Arg Ile Lys Glu Ile Leu Asn Lys Glu Phe 
705                 710                 715                 720 


Gly Ile Gln His Val Ser His Asn Asp Ile Val Arg Tyr Lys Leu Tyr 
                725                 730                 735     


Cys Glu Leu Lys Asn Asn Gly Tyr Lys Thr Leu Tyr Ser Asp Thr Tyr 
            740                 745                 750         


Ile Pro Gln Glu Lys Leu Phe Ser Lys Glu Val Asp Ile Glu His Ile 
        755                 760                 765             


Leu Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu 
    770                 775                 780                 


Glu Tyr Arg Ser Val Asn Ile Glu Lys Ser Asn Gln Thr Ala Trp Asp 
785                 790                 795                 800 


Tyr Val Ala Ala Lys Trp Gly Glu Glu Gly Ser Leu Arg Tyr Lys Lys 
                805                 810                 815     


Arg Val Asp Asp Leu Leu Ala Ala Gly Val Ile Gly Lys Ser Lys His 
            820                 825                 830         


Asp Lys Leu Leu Met Lys Gln Glu Asp Ile Pro Thr Asp Phe Ile Asn 
        835                 840                 845             


Arg Asp Leu Arg Asp Thr Gln Tyr Ile Ala Arg Lys Ala Arg Glu Ile 
    850                 855                 860                 


Leu Glu Asp Leu Val Pro Met Val Val Ser Thr Thr Gly Ala Val Thr 
865                 870                 875                 880 


Asp Arg Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Gln Glu Leu 
                885                 890                 895     


Asn Trp Asp Lys Tyr Ala Met Lys Gly Leu Thr Glu Glu Trp Thr Asp 
            900                 905                 910         


Lys Asp Gly Arg Arg Ile Ser Arg Ile Lys Glu Trp Thr Lys Arg Asn 
        915                 920                 925             


Asp His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr Lys 
    930                 935                 940                 


His Ser Phe Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Ser Asp Lys 
945                 950                 955                 960 


Ser Gly Ser Ile Tyr Ala Ile Glu Gln Lys Glu Leu Tyr Arg Asp Asn 
                965                 970                 975     


His Gly Lys Leu Arg Phe Ile Met Pro Phe Glu Gly Phe Arg Ser Glu 
            980                 985                 990         


Ala Lys Arg Gln Leu Glu Asn Ile  Leu Val Ser Ile Lys  Ala Lys Asn 
        995                 1000                 1005             


Lys Val  Val Thr Arg Asn Val  Asn Asn Ala Arg Arg  Gly Ser Gly 
    1010                 1015                 1020             


Arg Gln  Ala Lys Val Gln Leu  Thr Pro Arg Gly Gln  Leu His Asn 
    1025                 1030                 1035             


Glu Thr  Ile Tyr Gly Ser Ile  His Arg Tyr Val Thr  Lys Leu Glu 
    1040                 1045                 1050             


Lys Val  Gly Gly Asn Phe Asp  Gly Glu Gln Ile Ala  Lys Val Ala 
    1055                 1060                 1065             


Asn Leu  Arg Tyr Arg Glu Ala  Leu Gln Ala Arg His  Glu Ala Phe 
    1070                 1075                 1080             


Gly Gly  Asp Ala Lys Lys Ala  Phe Thr Gly Lys Asn  Ala Leu Asp 
    1085                 1090                 1095             


Lys Asn  Pro Leu Tyr Leu Lys  Asp Thr Arg Thr Glu  Val Pro Leu 
    1100                 1105                 1110             


Lys Val  Lys Thr Val Ser Thr  Glu Thr Leu Tyr Thr  Ile Arg Lys 
    1115                 1120                 1125             


Ala Val  Ala Pro Asp Leu Asn  Val Asp Lys Val Ile  Asp Ala Lys 
    1130                 1135                 1140             


Val Arg  Lys Val Leu Glu Glu  Arg Leu Gln Glu Tyr  Gly Gly Asp 
    1145                 1150                 1155             


Pro Lys  Lys Ala Phe Val Asn  Leu Asp Glu Asn Pro  Ile Tyr Leu 
    1160                 1165                 1170             


Asn Lys  Ala Lys Gly Ile Thr  Ile Lys Arg Val Ala  Ile Thr Gly 
    1175                 1180                 1185             


Val Asn  Asn Ala Ile Ala Leu  His Asp Lys Lys Asp  Val Gly Gly 
    1190                 1195                 1200             


His Val  Met Leu Asp Ala Ser  Gly Arg Lys Gln Pro  Val Asp Phe 
    1205                 1210                 1215             


Val Asn  Thr Gly Asn Asn His  His Val Ala Ile Tyr  Arg Asp Ala 
    1220                 1225                 1230             


Glu Gly  Ser Leu Gln Glu Val  Val Val Ser Phe Tyr  Glu Ala Val 
    1235                 1240                 1245             


Ala Arg  Val Ser Leu Gly Leu  Pro Val Ile Asp Arg  Glu Tyr Arg 
    1250                 1255                 1260             


Lys Glu  Asp Gly Trp Gln Phe  Leu Phe Thr Met Lys  Gln Asn Glu 
    1265                 1270                 1275             


Tyr Phe  Val Phe Pro Asn Pro  Glu Thr Gly Phe Asn  Pro Gln Glu 
    1280                 1285                 1290             


Val Asp  Leu Thr Asp Glu Thr  Asn Tyr Ala Leu Ile  Ser Pro Asn 
    1295                 1300                 1305             


Leu Phe  Arg Val Gln Lys Phe  Thr Ile Lys Asp Tyr  Phe Phe Arg 
    1310                 1315                 1320             


His His  Leu Glu Thr Thr Val  Glu Asp Arg Lys Glu  Leu Lys Asp 
    1325                 1330                 1335             


Met Thr  Trp Lys Arg Cys Gly  Leu Gly Gly Ile Ile  Gly Ile Val 
    1340                 1345                 1350             


Lys Val  Arg Val Asn His Ile  Gly Arg Ile Val Ser  Val Gly Glu 
    1355                 1360                 1365             


Tyr 
    


<210>  1008
<211>  1369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1008

Met Ala Lys Thr Tyr Asn Ile Gly Leu Asp Ile Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Trp Ala Val Val Asp Asp Gln Asn His Leu Met Ser Ile Lys Gly 
            20                  25                  30          


Lys Tyr Gly Tyr Gly Val Arg Leu Tyr Asp Glu Gly Lys Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Ser Leu Leu Arg Glu Ile Phe Glu Pro Gln Ile Thr Pro 
65                  70                  75                  80  


Leu Asp Asn Thr Phe Phe Leu Arg Gln Lys Gln Ser Ser Leu Ser Ser 
                85                  90                  95      


Lys Asp Asn Arg Lys Leu Tyr Pro Glu Met Ser Leu Phe Asn Asp Arg 
            100                 105                 110         


Thr Asp Thr Asp Phe Tyr Thr Gln Tyr Pro Thr Ile Tyr His Leu Ser 
        115                 120                 125             


Arg Asp Leu Met Thr Lys Asn Lys Gln Phe Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Met Tyr His Ile Val Lys Tyr Arg Gly His Phe Leu Asn Glu 
145                 150                 155                 160 


Ser Pro Val Ser Ser Phe Lys Ser Ser Glu Ile Lys Leu Ala Glu Asn 
                165                 170                 175     


Phe Glu Thr Leu Asn Gln Ile Phe Asn Glu Arg Phe Asn Glu Ser Met 
            180                 185                 190         


Phe Gln Leu Ala Val Asn Asp Phe Asp Asp Leu Lys Ala Leu Leu Leu 
        195                 200                 205             


Asn Asp Gln Gln Ser Ala Ser Asn Arg Gln Lys Lys Ala Leu Gly Met 
    210                 215                 220                 


Ile Tyr Val Pro Asn Glu Asn Lys Ser Leu Glu Lys Gln Asn Lys Ala 
225                 230                 235                 240 


Ile Ala Thr Glu Leu Leu Lys Ala Ile Leu Gly Leu Lys Ala Lys Phe 
                245                 250                 255     


Asn Val Leu Thr Gly Ile Glu Val Asp Asp Ala Lys Pro Trp Thr Leu 
            260                 265                 270         


Ser Phe Asn Ser Glu Ser Phe Asp Glu Asp Ile Asp Lys Ala Ala Thr 
        275                 280                 285             


Asn Ile Asp Asp Asp Ser Arg Glu Ile Ile Ala Ile Leu Gln Gly Leu 
    290                 295                 300                 


Tyr Ser Gly Ile Leu Leu Ala Gly Ile Ile Pro Glu Asn Gln Ser Leu 
305                 310                 315                 320 


Ser Glu Ala Met Val Glu Lys Tyr Asp Gln His Ala Ala His Leu Lys 
                325                 330                 335     


Gln Leu Lys Ser Ile Tyr Pro Leu Leu Ser Ile Ser Asp Lys Lys Ala 
            340                 345                 350         


Ile Gln Gln Ala Tyr Asn Tyr Tyr Ile Asp Gly Gln Asp Asn Ala Lys 
        355                 360                 365             


Ala Tyr Ser Arg Glu Asp Phe Tyr Ala Ala Thr Thr Lys Ala Leu Lys 
    370                 375                 380                 


Ser Tyr Gln Asp Asn Glu Thr Ile Ala Glu Ile Leu Lys Leu Ile Glu 
385                 390                 395                 400 


Leu Glu Gln Phe Met Pro Lys Gln Arg Thr Lys Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Gln Gln Glu Leu Glu Arg Ile Ile Glu Asn Gln 
            420                 425                 430         


Gln Lys Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Glu Ser Arg 
        435                 440                 445             


Arg Lys Ile Ala Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ile Thr Ser Glu Gln Gln Gln Gln Ser 
465                 470                 475                 480 


Ser Asn Lys Lys Phe Ala Trp Met Ile Arg Lys Ala Glu Gly Lys Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Glu Lys Val Asp Arg Gln Ala Ser Ala Asn 
            500                 505                 510         


Glu Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu Ala Glu 
        515                 520                 525             


Asp Val Leu Pro Lys Gln Ser Leu Val Tyr Gln Arg Phe Glu Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Gly Ile Lys Ile Asp Asp Val Pro Ile Thr Val Glu 
545                 550                 555                 560 


Gln Lys Gln Arg Ile Phe Asn Asp Leu Phe Met Met Gln Ala Ser Val 
                565                 570                 575     


Ser Val Lys Asn Ile Gln Asp Tyr Leu Val Ser Lys Gly Tyr Phe Ala 
            580                 585                 590         


Ser Arg Pro Glu Ile Thr Gly Leu Ser Asp Glu Asn Lys Val Ile Ser 
        595                 600                 605             


Arg Leu Ser Thr Tyr His Asp Leu Lys Ala Ile Leu Gly Gly Ile Val 
    610                 615                 620                 


Asp Glu Ala Asn Lys Gln Ala Asp Leu Glu Lys Cys Val Glu Trp Ser 
625                 630                 635                 640 


Thr Ile Phe Glu Asp Gly Lys Ile Phe Val Glu Lys Leu Asn Glu Ile 
                645                 650                 655     


Glu Trp Leu Thr Asp Glu Gln Arg Lys Arg Leu Ser Ser Lys Arg Tyr 
            660                 665                 670         


Arg Gly Trp Gly Arg Leu Ser Asn Lys Leu Leu Asn Gln Ile Val Asn 
        675                 680                 685             


Glu Asn Gly Gln Arg Ile Met Asp Leu Leu Trp Asp Thr Thr Asp Asn 
    690                 695                 700                 


Phe Met Arg Ile Val His Ser Glu Glu Phe Asp Lys Leu Ile Ser Ala 
705                 710                 715                 720 


Ala Asn Gln Thr Leu Leu Ser Lys Asn Asp Val Gln Asp Val Ile Asn 
                725                 730                 735     


Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Ile Leu 
            740                 745                 750         


Leu Val Val Ser Asp Ile Gln Lys Ala Met Lys Asn Gln Ala Pro Ser 
        755                 760                 765             


Lys Ile Leu Ile Glu Phe Ala Arg Glu Asp Glu Ala Asn Pro Arg Leu 
    770                 775                 780                 


Ser Val Gln Arg Lys Arg Gln Val Glu Gln Val Tyr Gln Asn Ile Ser 
785                 790                 795                 800 


Lys Asp Ile Leu Ser Asp Ser Asp Val Lys Ala Glu Leu Lys Asp Leu 
                805                 810                 815     


Thr Asn Thr Ala Leu Ala Asn Thr Arg Leu Phe Leu Tyr Phe Met Gln 
            820                 825                 830         


Gly Gly His Asp Met Tyr Thr Gly Glu Thr Leu Asn Ile Asp Arg Leu 
        835                 840                 845             


Ser Glu Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Phe Ile Lys Asp 
    850                 855                 860                 


Asn Ser Leu Asp Asn Arg Val Leu Val Ser Ala Lys Met Asn Arg Ser 
865                 870                 875                 880 


Lys Ser Asp Gln Val Pro Leu Asn Asp Tyr Thr Glu Glu Arg Phe Gly 
                885                 890                 895     


Ser Lys Met Arg Val Gln Trp Glu Gln Met Leu Arg Ala Gly Leu Ile 
            900                 905                 910         


Thr Lys Arg Lys Tyr Asp Asn Leu Thr Leu Asn Pro Asp Arg Ile Ser 
        915                 920                 925             


Lys Tyr Ala Met Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg 
    930                 935                 940                 


Gln Val Ile Lys Leu Val Thr Asn Leu Leu Met Asp His Tyr Glu Asp 
945                 950                 955                 960 


Asp Glu Val Gln Leu Ile Thr Val Lys Ser Gly Leu Ile His Gln Met 
                965                 970                 975     


Arg Glu Met Phe Asp Phe Pro Lys Asn Arg Asn Leu Asn Asn His His 
            980                 985                 990         


His Ala Phe Asp Ala Tyr Leu Thr  Ala Phe Val Gly Ile  Tyr Leu Leu 
        995                 1000                 1005             


Lys Arg  Tyr Pro Lys Leu Thr  Pro Phe Phe Val Tyr  Gly Ser Tyr 
    1010                 1015                 1020             


Gln Lys  Asn Asn Thr Asn Asp  Lys Trp Arg Asn Phe  Asn Phe Leu 
    1025                 1030                 1035             


Asn Asp  Leu Lys Lys Glu Glu  Val Ile Asp Ser Asp  Ser His Glu 
    1040                 1045                 1050             


Val Ile  Trp Asn Lys Ala Gln  Gly Leu Val Glu Leu  Asp Lys Ile 
    1055                 1060                 1065             


Tyr Gln  Phe Lys Lys Ile Leu  Val Thr Arg Glu Val  His Glu Asn 
    1070                 1075                 1080             


Thr Gly  Ala Leu Tyr Asn Gln  Thr Leu Tyr Ser Ala  Lys Asp Asp 
    1085                 1090                 1095             


Lys Ala  Ser Gly Gln Gly Gly  Lys Gln Leu Ile Pro  Ala Lys Gln 
    1100                 1105                 1110             


Asp Arg  Pro Thr Ala Leu Tyr  Gly Gly Tyr Ser Gly  Lys Thr Val 
    1115                 1120                 1125             


Ala Tyr  Met Cys Ile Val Arg  Ile Lys Ser Lys Lys  Glu Asp Thr 
    1130                 1135                 1140             


Tyr Lys  Val Cys Gly Val Lys  Thr Ser Trp Val Ala  Glu Leu Glu 
    1145                 1150                 1155             


Arg Leu  Ser Asp Ser Asn Glu  Gln Leu Ala Ser Leu  Lys Ala Lys 
    1160                 1165                 1170             


Leu Met  Pro Lys Phe Thr Lys  Asn Lys Lys Gln Lys  Gly Lys Thr 
    1175                 1180                 1185             


Ile Gln  Val Thr Glu Asp Phe  Glu Ile Ile Val Pro  Arg Val Leu 
    1190                 1195                 1200             


Ile Asn  Gln Arg Phe Tyr Asp  Asn Gly Gln Glu Ile  Thr Leu Gly 
    1205                 1210                 1215             


Ser Ala  Thr Tyr Lys His Asn  Glu Gln Glu Leu Met  Leu Asn Lys 
    1220                 1225                 1230             


Lys Ala  Val Lys Leu Leu Asn  Gly Gln Leu Ser Leu  Val Glu Ala 
    1235                 1240                 1245             


Glu Thr  Leu Ala Pro Gln Val  Tyr Asp Glu Ile Leu  Lys Gln Val 
    1250                 1255                 1260             


Met Lys  Tyr Phe Pro Leu Tyr  Asp Thr Asn Gln Phe  Arg Thr Lys 
    1265                 1270                 1275             


Leu Lys  Met Gly Lys Glu Gln  Phe Ile Lys Leu Pro  Trp Lys Asn 
    1280                 1285                 1290             


Lys Trp  Asp Gly Asn Lys Leu  Ile His Val Gly Gln  Gln Thr Val 
    1295                 1300                 1305             


Leu Asp  Arg Ile Leu Ile Gly  Leu His Ala Asn Ala  Ala Val Ser 
    1310                 1315                 1320             


Asp Leu  Ser Thr Leu Lys Val  Thr Thr Pro Phe Gly  Lys Leu Gln 
    1325                 1330                 1335             


Gln Pro  Ser Gly Ile Ala Leu  Ser Leu Asn Thr Lys  Ile Ile Tyr 
    1340                 1345                 1350             


Gln Ser  Pro Thr Gly Leu Phe  Glu Arg Arg Val Ala  Leu Arg Gly 
    1355                 1360                 1365             


Leu 
    


<210>  1009
<211>  1369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1009

Met Asp Lys Pro Tyr Asn Ile Gly Leu Asp Ile Gly Ser His Ser Ile 
1               5                   10                  15      


Gly Trp Thr Val Val Asp Asp Asn Ser Lys Leu Val Arg Ile Lys Gly 
            20                  25                  30          


Lys Leu Gly Tyr Gly Val Arg Leu Phe Asp Glu Gly Lys Thr Ala Glu 
        35                  40                  45              


Glu Arg Arg Gln Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Lys 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Ile Phe Glu Pro His Ile Ala Pro 
65                  70                  75                  80  


Leu Asp Glu Thr Phe Phe Leu Arg Gln Lys Gln Ser Asn Leu Val Pro 
                85                  90                  95      


Thr Gln Lys Lys Leu Pro Gln Leu Ser Leu Phe Asn Asp Arg Ser Asp 
            100                 105                 110         


Ala Glu Phe Tyr His Lys Tyr Pro Thr Ile Tyr His Leu Arg Asn Ala 
        115                 120                 125             


Leu Met Thr Glu Lys Arg Gln Phe Asp Leu Arg Glu Ile Tyr Leu Ala 
    130                 135                 140                 


Ile His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn Ala Ala Pro 
145                 150                 155                 160 


Ala Ala Ser Phe Lys Ser Gly Lys Phe Asp Leu Glu Asn Lys Phe Asp 
                165                 170                 175     


Gln Leu Asn Leu Leu Phe Ala Ser Ala Tyr Ser Asp Leu Asp Phe Thr 
            180                 185                 190         


Leu Arg Thr Asp Asn Val Asp Gln Val Lys Ala Ile Leu Leu Asn Glu 
        195                 200                 205             


Asp Leu Ser Ala Ser Thr Arg Gln Arg Gln Ala Leu Pro Leu Ile Tyr 
    210                 215                 220                 


Gln Thr Ser Ala Asp Gln Lys Ala Ala Lys Lys Leu Thr Lys Ser Leu 
225                 230                 235                 240 


Ala Thr Glu Leu Leu Lys Ala Ile Leu Gly Leu Lys Ala Lys Phe Asp 
                245                 250                 255     


Val Leu Thr Gly Ile Glu Thr Asp Asp Thr Lys Ala Trp Arg Leu Thr 
            260                 265                 270         


Phe Asn Ala Glu Asp Phe Asp Asp Lys Leu Asn Glu Leu Asn Val Thr 
        275                 280                 285             


Asp Asn Glu Ala Ala Thr Gln Ile Ile Glu Ile Leu Arg Glu Leu Tyr 
    290                 295                 300                 


Ser Ser Ile Leu Leu Ala Ala Ile Val Pro Glu Asn Tyr Thr Leu Ser 
305                 310                 315                 320 


Gln Ala Met Met Ala Lys Tyr Asn Ala His Lys Gln His Leu Ser Leu 
                325                 330                 335     


Leu Arg Gln Val Lys Thr Thr Leu Pro Ala Lys Lys Gln Lys Glu Leu 
            340                 345                 350         


Gln Ala Ala Tyr Asp Asp Tyr Ile Asp Gln His Leu Pro Leu Asp Lys 
        355                 360                 365             


Phe Tyr Ala Lys Leu Thr Lys Val Leu Pro Lys Glu Lys Asp Thr Asp 
    370                 375                 380                 


Asn Pro Gln Ile Ile Gln Ile Leu Asn Leu Ile Asp Leu Asp Gln Phe 
385                 390                 395                 400 


Leu Pro Lys Gln Arg Thr Lys Asp Asn Gly Ala Ile Pro His Gln Leu 
                405                 410                 415     


His Gln Gln Glu Leu Asp Gln Ile Ile Asp Asn Gln Lys Ala Tyr Tyr 
            420                 425                 430         


Pro Trp Leu Ala Gln Leu Asn Pro Asn Glu Lys Arg Gln Lys Ile Ala 
        435                 440                 445             


Lys Tyr Lys Leu Asp Glu Leu Val Ala Phe Arg Val Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Leu Ile Glu Ala Lys Thr Gln Gln Lys Thr Ser Gly Ala Asn 
465                 470                 475                 480 


Phe Ala Trp Met Ile Arg Lys Glu Pro Gly Thr Thr Thr Pro Ile Thr 
                485                 490                 495     


Pro Trp Asn Phe Asp Glu Lys Val Asn Arg Gln Ala Ser Ala Asn Glu 
            500                 505                 510         


Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu Gly Glu Asp 
        515                 520                 525             


Val Leu Pro Lys His Ser Leu Leu Tyr Gln Arg Phe Glu Val Leu Asn 
    530                 535                 540                 


Glu Leu Asn Gly Ile Lys Ile Asp Gln Lys Arg Val Thr Val Arg Gln 
545                 550                 555                 560 


Lys Gln Ala Ile Phe Asn Asp Leu Phe Leu Lys Gln Pro Asn Val Thr 
                565                 570                 575     


Val Lys Lys Leu Gln Gln Tyr Leu Gln Ala Lys Gly Glu Tyr Ala Ser 
            580                 585                 590         


Ala Pro Ile Ile Thr Gly Leu Ser Asp Pro Lys Lys Phe Asn Ser Gln 
        595                 600                 605             


Leu Ser Thr Tyr His Glu Tyr Lys Lys Ile Phe Gly Gly Leu Ile Asp 
    610                 615                 620                 


Asp Pro Asp Lys Gln Ala Asp Phe Glu Lys Cys Ile Glu Trp Ser Thr 
625                 630                 635                 640 


Ile Phe Glu Asp Ser Gln Ile Leu Lys Asp Lys Leu Lys Glu Ile Asn 
                645                 650                 655     


Trp Leu Thr Pro Glu Gln Arg His Lys Val Ala Asn Lys Arg Tyr Gln 
            660                 665                 670         


Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gln Leu Val Asp Tyr 
        675                 680                 685             


Asn Gly Gln Arg Ile Ile Asp Ile Leu Trp Asn Thr Thr Asp Asn Phe 
    690                 695                 700                 


Met Gln Ile Val His Ser Glu Ala Phe Asp Lys Ala Ile Ile Lys Glu 
705                 710                 715                 720 


Asn Gln Ala Leu Leu Thr Gln Tyr Asn Pro Gln Ala Val Ile Asp Asp 
                725                 730                 735     


Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Ile Leu Leu 
            740                 745                 750         


Val Val Asn Asp Ile Gln Lys Ala Leu Lys Gly Gln Ala Pro Ser Arg 
        755                 760                 765             


Ile Leu Ile Glu Phe Ala Arg Glu Gly Gln Glu Asn Pro Arg Leu Ser 
    770                 775                 780                 


Thr Gln Arg Lys Gln Gln Val Glu Asn Ala Tyr Lys Glu Ala Ser Asp 
785                 790                 795                 800 


Ala Leu Leu Ser Ser Ala Glu Val Arg Gln Glu Leu Lys Gly Leu Asp 
                805                 810                 815     


Asn Lys Ala Leu Ala Asn Thr Arg Leu Phe Leu Tyr Phe Met Gln Gly 
            820                 825                 830         


Gly Arg Asp Ile Tyr Thr Gly Lys Ser Leu Asn Ile Asp Asp Leu Ser 
        835                 840                 845             


Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Phe Ile Lys Asp Asn 
    850                 855                 860                 


Ser Leu Asp Asn Arg Val Leu Val Ser Ala Lys Met Asn Arg Ser Lys 
865                 870                 875                 880 


Ser Asp Gln Val Pro Leu Asn Asp Phe Ala Glu Ile Arg Tyr Gly Lys 
                885                 890                 895     


Gln Met Arg Gln Gln Trp Glu Lys Met Leu Arg Ala Gly Leu Ile Thr 
            900                 905                 910         


Arg Lys Lys Tyr His Asn Leu Thr Leu Asn Pro Glu Ser Ile Asn Lys 
        915                 920                 925             


Tyr Thr Ala Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Val Thr Lys Leu Ala Thr Asn Leu Leu Val Asp Gln Tyr Ala Glu Asp 
945                 950                 955                 960 


Asp Val Gln Leu Ile Thr Val Lys Ser Asn Leu Thr His Gln Met Arg 
                965                 970                 975     


Glu Glu Phe Asp Phe Pro Lys Asn Arg Asn Val Asn Asn His His His 
            980                 985                 990         


Ala Phe Asp Ala Tyr Leu Thr Ala  Phe Val Gly Leu Tyr  Leu Leu Lys 
        995                 1000                 1005             


Arg Tyr  Pro Lys Leu Ala Pro  Tyr Phe Val Tyr Gly  Asp Tyr Gln 
    1010                 1015                 1020             


Lys Gly  Ala Ser Gln Asn Ser  Lys Trp Arg Asn Phe  Asn Phe Leu 
    1025                 1030                 1035             


Gly Asn  Leu His Arg Glu Lys  Val Val Asp Lys Asn  Thr Gly Glu 
    1040                 1045                 1050             


Ile Ile  Trp Gln Arg Asp Ala  Gly Leu Gly Glu Leu  Asn Arg Ile 
    1055                 1060                 1065             


Tyr Asn  Phe Lys Lys Ile Leu  Val Thr Arg Ala Leu  Arg Val Asn 
    1070                 1075                 1080             


Arg Gly  Pro Met Phe Lys Gln  Thr Ile Tyr Lys Ala  Glu Glu Asp 
    1085                 1090                 1095             


Lys Ser  Ser Gly Arg Gly Ser  Lys Gln Leu Ile Pro  Thr Lys Lys 
    1100                 1105                 1110             


Asn Arg  Pro Thr Glu Leu Tyr  Gly Gly Tyr Thr Asn  Lys Glu Thr 
    1115                 1120                 1125             


Ala Tyr  Leu Cys Leu Val Lys  Glu Pro His Lys Lys  Gly Asp Ile 
    1130                 1135                 1140             


Tyr Arg  Ile Cys Ser Val Leu  Asn Met Trp Leu Thr  Glu Leu Asp 
    1145                 1150                 1155             


Arg Ile  Ala Asp Pro Ile Ala  Lys Ala Gln Lys Leu  Thr Glu Leu 
    1160                 1165                 1170             


Leu Thr  Pro Gln Phe Thr Thr  Thr Lys Lys Lys Gln  Gly Gln Lys 
    1175                 1180                 1185             


Ile Pro  Val Val Lys Pro Phe  Lys Val Val Ile Pro  Lys Ile Leu 
    1190                 1195                 1200             


Ile Asn  Gln Arg Phe Phe Ser  Arg Gly Gln Glu Leu  Thr Val Ala 
    1205                 1210                 1215             


Ser Pro  Met Tyr Met His Asn  Glu Gln Glu Leu Phe  Leu Asn Lys 
    1220                 1225                 1230             


Lys Thr  Ile Lys Leu Leu Asn  Gly Gln Leu Ala Lys  Asn Glu Ala 
    1235                 1240                 1245             


His Glu  Leu Ala Pro Lys Ile  Tyr Gln Gln Ile Leu  Asp Gln Val 
    1250                 1255                 1260             


Asn Asp  Tyr Phe Pro Leu Tyr  Asp Met Tyr Lys Phe  Arg Asp Thr 
    1265                 1270                 1275             


Leu Asn  Ala Ser Lys Glu Ile  Phe Val Asn Leu Pro  Trp Gln Asp 
    1280                 1285                 1290             


Gln Arg  Gln Asp Lys Lys Ile  Ser Gln Ser Gly Gln  Lys Ser Ile 
    1295                 1300                 1305             


Leu Asn  Lys Ile Leu Ile Gly  Leu His Ala Asn Ala  Ala Arg Ile 
    1310                 1315                 1320             


Lys Leu  Thr Glu Leu Asn Phe  Lys Ser Asp Phe Gly  Leu Leu Ser 
    1325                 1330                 1335             


Gln Arg  Ser Gly Ile Ala Phe  Ser Pro Asp Asp Gln  Ile Ile Tyr 
    1340                 1345                 1350             


Gln Ser  Pro Thr Gly Leu Phe  Glu Arg Arg Val Thr  Leu Lys Asp 
    1355                 1360                 1365             


Leu 
    


<210>  1010
<211>  1369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1010

Met Lys Val Asn Asn Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Trp Val Ala Met Gly Glu Asp Gly Lys Pro Leu Arg Val Lys 
            20                  25                  30          


Gly Lys Thr Ala Ile Gly Ala Arg Leu Phe Arg Glu Gly Asn Pro Ala 
        35                  40                  45              


Ala Asp Arg Arg Met Phe Arg Thr Thr Arg Arg Arg Leu Asn Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Lys Leu Leu Asp Glu Ile Phe Asp Pro Tyr Ile Thr 
65                  70                  75                  80  


Pro Val Asp Ser Thr Phe Phe Ala Arg Leu Lys Gln Ser Asn Trp Ser 
                85                  90                  95      


Pro Lys Asp Ser Arg Lys Ala Phe Lys Asp Ser Thr Leu Phe Pro Asp 
            100                 105                 110         


Leu Thr Asp Lys Gln Tyr His Leu Asp Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg His Ala Leu Met Thr Gln Asp Lys Gln Phe Asp Ile Arg Met Val 
    130                 135                 140                 


Tyr Leu Ala Leu His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn 
145                 150                 155                 160 


Ser Thr Pro Val Asp Ser Phe Lys Ala Ser Lys Val Asn Phe Asp Asp 
                165                 170                 175     


Gln Phe Lys Lys Leu Asn Glu Leu Tyr Thr Ala Ile Asn Pro Glu Glu 
            180                 185                 190         


Ser Phe Gln Ile Asn Phe Ala Asn Ser Glu Lys Ile Gly Gln Gln Phe 
        195                 200                 205             


Leu Asp Pro Ser Ile Arg Lys Phe Asp Lys Lys Lys Gln Ile Ser Lys 
    210                 215                 220                 


Ile Val Pro Val Thr Met Asp Asp Lys Ala Val Asp Lys Leu Asn Gly 
225                 230                 235                 240 


Lys Ile Ala Ser Glu Ile Val Asn Ala Ile Leu Gly Tyr Lys Ser Lys 
                245                 250                 255     


Leu Asp Val Ala Val Gln Cys Asp Pro Glu Asp Ser Lys Pro Trp Ala 
            260                 265                 270         


Val Lys Phe Asp Asp Glu Asp Ile Asp Ala Lys Leu Gln Lys Ile Leu 
        275                 280                 285             


Pro Glu Met Asp Glu Asn Gln Gln Ser Ile Ile Gly Ile Ile Gln Asp 
    290                 295                 300                 


Leu Tyr Ser Gln Val Thr Leu Asn Gln Ile Val Pro Asn Gly Met Ser 
305                 310                 315                 320 


Leu Ser Glu Ser Met Ile Lys Lys Tyr Asn Asp His His Asp His Leu 
                325                 330                 335     


Lys Leu Tyr Lys Lys Leu Ile Gly Gln Ile Ser Asp Ser Lys Lys Lys 
            340                 345                 350         


Thr Ala Leu Lys Lys Ala Tyr Asp Gln Tyr Val Gly Asn Asp Gly Lys 
        355                 360                 365             


Val Ile Glu Gln Asp Asp Phe Tyr Ser Ser Val Lys Lys Asn Leu Asp 
    370                 375                 380                 


Asp Ser Glu Leu Ser Lys Gln Ile Met Asp Leu Ile Asp Ala Asp Lys 
385                 390                 395                 400 


Phe Met Pro Lys Gln Arg Thr Ser Gln Asn Gly Val Ile Pro His Gln 
                405                 410                 415     


Leu His Gln Arg Glu Leu Asp Glu Ile Ile Glu His Gln Ser Lys Tyr 
            420                 425                 430         


Tyr Pro Trp Leu Ala Glu Ser Asn Pro Asn Lys His Asp Leu His Leu 
        435                 440                 445             


Ala Lys Tyr Lys Ile Glu Glu Leu Val Ala Phe Arg Ile Pro Tyr Tyr 
    450                 455                 460                 


Val Gly Pro Met Ile Thr Pro Glu Asp Gln Lys Lys Ser Gly Ala Ala 
465                 470                 475                 480 


Val Phe Ser Trp Met Lys Arg Lys Glu Pro Gly Gln Ile Thr Pro Trp 
                485                 490                 495     


Asn Phe Asp Gln Lys Val Asp Arg Met Glu Ser Ala Asn Lys Phe Ile 
            500                 505                 510         


Lys Arg Met Thr Thr Lys Asp Thr Tyr Leu Ile Gly Glu Asp Val Leu 
        515                 520                 525             


Pro Asp Glu Ser Leu Leu Tyr Glu Lys Phe Lys Val Leu Asn Glu Leu 
    530                 535                 540                 


Asn Met Val Arg Val Asn Gly Asp Ser Leu Lys Val Ala Asp Lys Gln 
545                 550                 555                 560 


Ala Ile Tyr Gln Asp Leu Phe Glu Asn Tyr Lys His Ile Ser Val Lys 
                565                 570                 575     


Lys Met Gln Asn Tyr Ile Lys Asp Lys Thr Gly Leu Pro Asn Asn Pro 
            580                 585                 590         


Asp Ile Ser Gly Leu Ser Asp Pro Asp His Phe Asn Asn Ser Leu Gly 
        595                 600                 605             


Thr Tyr Asn Asp Phe Lys Lys Leu Phe Gly Asp Lys Val Asp Asp Pro 
    610                 615                 620                 


Asp Leu Gln Asp Asp Phe Glu Lys Ile Val Glu Trp Ser Thr Val Phe 
625                 630                 635                 640 


Glu Asp Lys Lys Ile Leu Arg Ala Lys Leu Asn Glu Ile Ser Trp Leu 
                645                 650                 655     


Ser Asp Lys Gln Lys Asp Asp Leu Glu Ser Ser Arg Tyr Gln Arg Trp 
            660                 665                 670         


Gly Arg Leu Ser Lys Lys Leu Leu Thr Gly Ile Val Asn Asp Gln Gly 
        675                 680                 685             


Glu Arg Ile Ile Asp Glu Leu Trp Asn Thr Asn Lys Asn Phe Met Gln 
    690                 695                 700                 


Ile Gln Ser Asp Asp Asp Phe Ala Lys Arg Ile His Glu Ala Asn Ala 
705                 710                 715                 720 


Asp Gln Met Lys Ala Thr Asp Val Glu Asp Val Leu Ala Asp Ala Tyr 
                725                 730                 735     


Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Val Lys Val Val 
            740                 745                 750         


Asp Asp Ile Gln Arg Ala Met Gly Gly Val Ala Pro Lys Phe Ile Ser 
        755                 760                 765             


Ile Glu Phe Thr Arg Ser Glu Asp Arg Asn Pro Arg Arg Thr Ile Ser 
    770                 775                 780                 


Arg Glu Arg Gln Leu Glu Asp Thr Leu Lys Asp Thr Ala Lys Thr Leu 
785                 790                 795                 800 


Val Lys Ser Met Asn Pro Asp Ile Leu Ser Glu Leu Asp Asn Ala Ser 
                805                 810                 815     


Lys Ser Lys Lys Gly Leu Thr Asp Arg Leu Tyr Leu Tyr Phe Thr Gln 
            820                 825                 830         


Leu Gly Arg Asp Met Tyr Thr Gly Lys Pro Ile Asn Ile Asp Glu Ile 
        835                 840                 845             


Ser Asn Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Phe Ile Lys Asp 
    850                 855                 860                 


Asp Ser Leu Ser Asn Arg Val Leu Val Ser Lys Ala Val Asn Asn Gly 
865                 870                 875                 880 


Lys Ser Ala Asn Val Pro Leu Lys Leu Phe Gly Val Lys Met Ala His 
                885                 890                 895     


Phe Trp Lys Gln Leu Ala Glu Ala Gly Leu Ile Asn Lys Arg Lys Leu 
            900                 905                 910         


Lys Asn Leu Gln Thr Asn Pro Asp Thr Ile Ser Lys Phe Ala Met His 
        915                 920                 925             


Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu 
    930                 935                 940                 


Val Ala Asn Ile Leu Gly Asp Lys Tyr Arg Asn Asp Asp Thr Lys Ile 
945                 950                 955                 960 


Ile Glu Ile Thr Ala Arg Met Asn His Gln Met Arg Asp Glu Phe Ala 
                965                 970                 975     


Phe Ile Lys Asn Arg Glu Ile Asn Asp Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Tyr Leu Thr Ala Phe Leu Gly Arg  Tyr Leu Tyr His Arg  Tyr Ile Lys 
        995                 1000                 1005             


Leu Arg  Pro Tyr Phe Val Tyr  Gly Asp Phe Lys Lys  Phe Arg Glu 
    1010                 1015                 1020             


Asp Lys  Val Thr Met Arg Asn  Phe Asn Phe Leu His  Asp Leu Thr 
    1025                 1030                 1035             


Asp Asp  Thr Gln Ala Arg Ile  Ala Asp Thr Glu Thr  Gly Glu Val 
    1040                 1045                 1050             


Ile Trp  Asp Arg Glu Thr Ser  Ile Lys Gln Leu Arg  Asp Val Tyr 
    1055                 1060                 1065             


His Tyr  Lys Phe Met Leu Ile  Ser Gln Glu Val Tyr  Thr Leu Arg 
    1070                 1075                 1080             


Gly Ala  Met Phe Asn Gln Thr  Ile Tyr Pro Ala Asp  Glu Ala Gly 
    1085                 1090                 1095             


Arg Lys  Lys Leu Ile Pro Thr  Lys Ala Asp Lys Ala  Val Asp Val 
    1100                 1105                 1110             


Tyr Gly  Gly Tyr Ser Gly Ser  Asn Asp Ala Tyr Met  Ala Ile Val 
    1115                 1120                 1125             


Arg Ile  His Asp Lys Lys Gly  Asp Lys Tyr Lys Val  Val Gly Val 
    1130                 1135                 1140             


Pro Met  Arg Ala Leu Asp Arg  Leu Asn Ala Ala Lys  Asn Val Ser 
    1145                 1150                 1155             


Asp Ala  Lys Tyr Asp Gln Val  Leu Lys Asp Val Leu  Thr Pro Gln 
    1160                 1165                 1170             


Phe Thr  Lys Ser Lys Lys Ser  Arg Lys Thr Gly Glu  Ile Ser Gln 
    1175                 1180                 1185             


Thr Val  Glu Asp Phe Glu Val  Val Leu Gly Lys Val  Met Tyr Arg 
    1190                 1195                 1200             


Gln Leu  Val Ile Asp Gly Asp  Lys Lys Phe Met Leu  Gly Ser Ser 
    1205                 1210                 1215             


Thr Tyr  Gln Tyr Asn Ala Lys  Gln Leu Val Leu Ser  Asp Glu Ser 
    1220                 1225                 1230             


Val Lys  Thr Leu Ala Ser Lys  Gly Lys Leu Asp Glu  Ala Gln Glu 
    1235                 1240                 1245             


Ser Ala  Ala Tyr Asp Asn Val  Tyr Asp Glu Ile Leu  Asp Lys Val 
    1250                 1255                 1260             


Asp Arg  Tyr Phe Ala Leu Tyr  Asp Gln Arg Asn Tyr  Arg Thr Asn 
    1265                 1270                 1275             


Leu His  Glu Gly Glu Gln Lys  Phe Ile Gly Leu Pro  Leu His Asp 
    1280                 1285                 1290             


Thr Phe  Asp Gly Ser Lys Lys  Val Ala Asn Gly Lys  Arg Glu Thr 
    1295                 1300                 1305             


Ile Ser  Asp Ile Leu Asn Gly  Leu His Ala Asn Ala  Thr Met Thr 
    1310                 1315                 1320             


Asn Leu  Lys Val Leu Gly Ile  Ser Ser Pro Phe Gly  Met Phe Gln 
    1325                 1330                 1335             


Ser His  Gly Pro Ile Asn Leu  Asn Pro Glu Thr Ile  Ile Leu Cys 
    1340                 1345                 1350             


Gln Ser  Pro Thr Gly Leu Phe  Glu Arg Arg Ile Ile  Leu Arg Asp 
    1355                 1360                 1365             


Leu 
    


<210>  1011
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1011

Met Lys Lys Arg Asn Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Trp Thr Ile Val Asp Asp Asn Ala Gln Ile Met Arg Ala Lys 
            20                  25                  30          


Gly Lys Asn Gly Tyr Gly Val Arg Ile Phe Ala Glu Gly Lys Thr Ala 
        35                  40                  45              


Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Arg Leu Leu Gln Glu Phe Phe Glu Pro Tyr Ile Leu 
65                  70                  75                  80  


Pro Ile Asp Glu Asn Phe Phe Met Arg Gln Lys Glu Ser Asn Leu Val 
                85                  90                  95      


Pro Gln Asp Pro Asn Lys His Phe Lys Gly Ser Ile Leu Phe Asn Asp 
            100                 105                 110         


Arg Thr Asp Gln Glu Phe His Glu Gln Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Tyr Ala Leu Met Thr Glu His Arg Gln Phe Asp Val Arg Glu Ile 
    130                 135                 140                 


Tyr Trp Ala Ile His His Ile Val Lys Tyr Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Ser Thr Leu Ala Ser Lys Phe Lys Gly Gly Glu Leu Arg Leu Ala Asp 
                165                 170                 175     


Lys Phe Glu Ser Leu Asn Lys Leu Phe Leu Arg Val Gln Ala Glu Thr 
            180                 185                 190         


Gly Phe Glu Leu Ser Glu Asp Asn Val Asp Lys Ile Lys Thr Val Leu 
        195                 200                 205             


Leu Asp Asn Gly Lys Ser Arg Leu Asp Arg Gln Arg Glu Val Leu Lys 
    210                 215                 220                 


Ile Val Tyr Leu Thr Ser Glu Asp Lys Asp Thr Glu Lys Lys Arg Lys 
225                 230                 235                 240 


Ala Val Ala Thr Glu Phe Ile Lys Ala Val Leu Gly Leu Lys Thr Lys 
                245                 250                 255     


Leu Phe Ile Val Leu Ala Lys Asn Pro Thr Ala Glu Thr Asp Trp Lys 
            260                 265                 270         


Phe Ser Phe Asp Ala Glu Asp Ile Asp Asp Lys Leu Asp Ser Ile Ser 
        275                 280                 285             


Glu Gln Leu Thr Asp Asp Asp His Glu Ile Val Glu Leu Ile Ser Glu 
    290                 295                 300                 


Ile His Ser Ile Ile Thr Leu Thr Gly Val Val Pro Glu Gly Met Gly 
305                 310                 315                 320 


Leu Ser Glu Ser Met Ile Ala Lys Tyr Asp Gln His Gln Leu Asp Leu 
                325                 330                 335     


Lys Lys Leu Lys Ser Leu Met Arg Asp Leu Asp Val Lys Gln Ala Arg 
            340                 345                 350         


Lys Leu Gln Asp Ala Tyr Asp Glu Tyr Ile Asp Gly Leu Lys Pro Ser 
        355                 360                 365             


Glu Glu Ala Phe Tyr Ser Ala Val Ser Ser Val Ile Gln Lys Met Asp 
    370                 375                 380                 


Asp Pro Arg Ala Lys Lys Ile Thr Ala Glu Ile Glu Leu Glu Glu Phe 
385                 390                 395                 400 


Met Pro Lys Gln Arg Thr Lys Ala Asn Gly Gln Ile Pro His Gln Leu 
                405                 410                 415     


His Gln Gln Glu Leu Asp Gln Ile Ile Glu Asn Gln Lys Glu Tyr Tyr 
            420                 425                 430         


Pro Trp Leu Ala Glu Glu Asn Pro Asn Lys Arg Glu His Asn Ala Ala 
        435                 440                 445             


Pro His Lys Leu Asp Gly Leu Val Thr Phe Arg Val Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Leu Ile Glu Ala Ser Val Gln Gln Lys Thr Ser Asn Ser Asn 
465                 470                 475                 480 


Phe Ala Trp Met Glu Arg Lys Val Lys Asn Asp Thr Ser Arg Ile Thr 
                485                 490                 495     


Pro Trp Asn Phe Glu Glu Lys Val Asp Arg Asn Ala Ser Ala Glu Asn 
            500                 505                 510         


Phe Ile Lys Arg Met Thr Thr Lys Asp Thr Tyr Leu Leu Asn Glu Asp 
        515                 520                 525             


Val Leu Pro Gln Gln Ser Leu Leu Tyr Gln Arg Tyr Glu Val Leu Asn 
    530                 535                 540                 


Glu Leu Asn Asn Val Arg Val Asn Asp Lys Lys Leu Glu Asn Asn Cys 
545                 550                 555                 560 


Lys Gln Asp Val Tyr Lys Asp Leu Phe Lys Asn Asn Thr Thr Val Ser 
                565                 570                 575     


Ile Lys Lys Phe Lys Asp Tyr Leu Val Ala Asn Cys Glu Tyr Ala Asp 
            580                 585                 590         


Asn Pro Lys Ile Glu Gly Leu Ala Asp Glu Ser Ile Phe Leu Ser Ser 
        595                 600                 605             


Leu Ser Thr Glu Asn Lys Phe Lys Asp Ile Phe Gly Ala Gln Ile Asp 
    610                 615                 620                 


Asp Pro Asn Tyr Phe Glu Asp Phe Glu His Ile Ile Glu Trp Ala Thr 
625                 630                 635                 640 


Ile Phe Glu Asp Asn Lys Ile Leu Arg Ala Lys Leu Asp Glu Ile Ser 
                645                 650                 655     


Trp Leu Asn Glu Ser Gln Lys Lys Glu Leu Ala Lys Leu Arg Met Arg 
            660                 665                 670         


Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Ala Glu Ile Thr Asp Glu 
        675                 680                 685             


Asn Gly Ser Arg Ile Ile Asp Leu Leu Trp Glu His Met Asp Asn Phe 
    690                 695                 700                 


Met Val Val Val Asn Gln Asp Val Phe Lys Glu Lys Ile Val Ala Glu 
705                 710                 715                 720 


Asn Lys Gln Val Phe Lys Glu Leu Asn Pro Leu Asp Ile Ile Asn Glu 
                725                 730                 735     


Gln Phe Thr Ser Pro Gln Asn Lys Lys Ala Leu Arg Gln Ile Leu Leu 
            740                 745                 750         


Val Val Ala Asp Ile Gln Lys Ala Met Asn Gly Val Thr Pro Ser Asn 
        755                 760                 765             


Ile Phe Ile Glu Phe Ala Arg Glu Asp Glu Arg Asn Pro Arg Arg Ser 
    770                 775                 780                 


Ile Gln Arg Leu Arg Gln Val Glu Asn Met Tyr Arg Asp Ile Ser Asp 
785                 790                 795                 800 


Thr Val Leu Ala Asp Pro Glu Val Arg Asn Glu Leu Ser Asn Ile Lys 
                805                 810                 815     


Asn Thr Glu Leu Ala Arg Asp Arg Val Phe Leu Tyr Phe Leu Gln Gly 
            820                 825                 830         


Gly Arg Asp Val Tyr Thr Gly Glu Pro Leu Asn Ile Asp Lys Leu Ser 
        835                 840                 845             


Glu Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Phe Ile Lys Asp Asn 
    850                 855                 860                 


Ser Leu Asp Asn Arg Val Leu Val Ser Ala Ser Leu Asn Arg Ser Lys 
865                 870                 875                 880 


Ser Asp Ser Val Pro Leu Asp Asp Phe Thr Ser Glu Met Phe Gly Arg 
                885                 890                 895     


Lys Met Glu Pro Leu Trp Glu Gln Met Gln Arg Ala Gly Leu Ile Ser 
            900                 905                 910         


Lys Lys Lys Leu Ala Asn Leu Lys Phe Asn Pro Glu Asn Ile Asn Lys 
        915                 920                 925             


Tyr Thr Arg Asn Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln 
    930                 935                 940                 


Val Ile Lys Leu Ala Ala Asn Ile Leu Lys Asp His Tyr Gly Glu Glu 
945                 950                 955                 960 


Thr Glu Ile Val Asn Val Lys Ala Gly Leu Thr His Gln Phe Arg Lys 
                965                 970                 975     


Asp Phe Asp Phe Pro Lys Asn Arg Asn Val Asn Asn Tyr His His Ala 
            980                 985                 990         


Phe Asp Ala Tyr Leu Thr Ala Leu  Val Gly Thr Tyr Leu  Leu Lys Lys 
        995                 1000                 1005             


Tyr Pro  Lys Leu Glu Ser Tyr  Phe Val Tyr Gly Lys  Phe Lys Lys 
    1010                 1015                 1020             


Gly Asp  Gly Ile Thr Asn Leu  Lys Asn Phe Asn Phe  Leu His Gly 
    1025                 1030                 1035             


Phe Asn  Lys Pro Gly Lys Ile  Ile Asn Lys Glu Thr  Gly Glu Ile 
    1040                 1045                 1050             


Leu Phe  Asp Arg Asp Lys Gln  Leu Gly Tyr Met Asn  Ser Ile Tyr 
    1055                 1060                 1065             


Asp Leu  Arg Lys Val Ile Val  Thr His Glu Leu Ser  Thr Arg His 
    1070                 1075                 1080             


Gly Ala  Met Tyr Asp Gln Thr  Leu Tyr Lys Ala Ser  Asp Asp Lys 
    1085                 1090                 1095             


Asp Ser  Gly Arg Gly Gly Lys  Gln Leu Ile Arg Lys  Lys Asp Asn 
    1100                 1105                 1110             


Leu Pro  Thr Glu Ile Tyr Gly  Gly Tyr Thr Gly Ser  Thr Asp Ala 
    1115                 1120                 1125             


Phe Met  Ser Ile Ile Arg Met  Lys Lys Lys Asp Lys  Leu Tyr Tyr 
    1130                 1135                 1140             


Lys Val  Ile Gly Val Pro Thr  Arg Val Ala His Glu  Val Leu Gln 
    1145                 1150                 1155             


Ser Asp  Gly Lys Ile Asn Ile  Phe Thr Leu Asn Lys  Phe Ile Glu 
    1160                 1165                 1170             


Gln Lys  Leu Thr Lys Glu Lys  Val Asn Lys Lys Thr  Gly Ala Ile 
    1175                 1180                 1185             


Thr Arg  Ile Thr Asp Trp Phe  Glu Leu Val Ile Ala  Lys Val Met 
    1190                 1195                 1200             


Tyr Gly  Gln Val Ile Asp Asp  Gly Gly Gln Pro Phe  Met Leu Gly 
    1205                 1210                 1215             


Ser Ser  Thr Leu Pro Tyr Asn  Val Arg Glu Leu Tyr  Leu Ala Lys 
    1220                 1225                 1230             


Asp Leu  Val Lys Val Ile Asn  Lys Gly Ser Thr Ser  Lys Ser Ser 
    1235                 1240                 1245             


Tyr Leu  Asp Val Tyr Asp Glu  Leu Val Lys Ser Leu  Asn Lys Tyr 
    1250                 1255                 1260             


Phe Pro  Met Tyr Asp Arg Asn  Met Phe Arg Gln Lys  Ile Thr Asp 
    1265                 1270                 1275             


Ala Arg  Asp Lys Phe Glu Lys  Leu Pro Lys Arg Asp  Lys Val Ser 
    1280                 1285                 1290             


Asn Gly  Lys Leu Gln Gln Ile  Gly Gln Val Thr Ile  Ile Asp Arg 
    1295                 1300                 1305             


Ile Leu  Ile Gly Leu His Thr  Asn Ala Gly Val Ser  Asn Leu Lys 
    1310                 1315                 1320             


Asp Leu  Gly Ile Ala Thr Pro  Phe Gly Phe Met Gln  Gln Gly Asn 
    1325                 1330                 1335             


Gly Ile  Ser Leu Thr Ser Thr  Ala Lys Ile Ile Tyr  Gln Ser Ser 
    1340                 1345                 1350             


Thr Gly  Leu Phe Glu Lys Ser  Lys Ala Leu Asp Asp  Leu Asn Pro 
    1355                 1360                 1365             


Leu Ala  
    1370 


<210>  1012
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1012

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Tyr Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Arg Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Lys Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Arg Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Leu Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Arg Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Leu Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Phe Leu Ala Glu Gly Phe Lys Asp Phe Gln Phe Leu Asn Arg Lys 
    530                 535                 540                 


Gln Lys Glu Thr Ile Phe Asn Ser Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Ser Phe Leu Asn Lys Val Asp Gly Tyr Glu 
                565                 570                 575     


Gly Ile Ala Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Gly Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Thr Asp Asn Glu Leu Ile Leu Glu Asp Ile Val Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Asp Ile Tyr Lys Asp 
625                 630                 635                 640 


Phe Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Ala Gly Leu Ser Phe Lys 
    690                 695                 700                 


Pro Ile Ile Asp Lys Ala Arg Thr Gly Ser His Ser Asp Asn Leu Lys 
705                 710                 715                 720 


Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Leu Ser Arg Leu Arg Gln Arg Leu Thr Thr Leu Arg Glu 
    770                 775                 780                 


Ser Leu Ala Asn Leu Lys Ser Asn Ile Leu Glu Glu Lys Lys Pro Lys 
785                 790                 795                 800 


Tyr Val Lys Asp Gln Val Glu Asn His His Leu Ser Asp Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp Leu Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Val Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Asp Asp Lys  Leu Met Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Asp Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Leu Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Gln  Lys Gln Glu Phe Val  Val Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Ile Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Asn Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Pro Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1013
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1013

Met Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Thr Lys Val Asp Glu 
                85                  90                  95      


Ser Phe Phe Tyr Arg Leu Asp Glu Ser Phe Leu Thr Thr Asp Glu Lys 
            100                 105                 110         


Asp Phe Glu Arg His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala 
        115                 120                 125             


Tyr His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Asn Tyr Leu Ala 
    130                 135                 140                 


Asp Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn 
                165                 170                 175     


Ala Glu Asn Thr Asp Val Gln Lys Leu Phe Thr Asp Phe Val Gly Val 
            180                 185                 190         


Tyr Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp 
        195                 200                 205             


Val Ala Ser Thr Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Leu Ile Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly 
225                 230                 235                 240 


Asn Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Met Asn 
                245                 250                 255     


Phe Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu 
                325                 330                 335     


Lys Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe 
            340                 345                 350         


Lys Asp Lys Asn Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val 
        355                 360                 365             


Lys Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Asn Thr Leu Ser Lys Ile 
    370                 375                 380                 


Asp Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Lys Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Val Arg Lys Asp Ser Arg Phe Ala 
    450                 455                 460                 


Trp Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Lys Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser His Val Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr Lys Ile 
        515                 520                 525             


Lys Tyr Val Asn Glu Gln Gly Lys Glu Ser Phe Phe Asp Ser Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 


Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg 
                565                 570                 575     


Ile Lys Asp Leu Ile Gly Leu Asp Lys Glu Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys Ala Phe 
        595                 600                 605             


Leu Asp Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu Gln Lys 
625                 630                 635                 640 


Tyr Ser Asp Ile Phe Thr Ala Asn Gln Leu Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asn Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Ser Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu 
    690                 695                 700                 


Pro Phe Lys Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp Val Asp 
705                 710                 715                 720 


Asp Ile Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys 
    770                 775                 780                 


Leu Gln Ser Ser Leu Lys Glu Leu Gly Ser Asn Ile Leu Asn Glu Glu 
785                 790                 795                 800 


Lys Pro Ser Tyr Ile Glu Asp Lys Val Glu Asn Ser His Leu Gln Asn 
                805                 810                 815     


Asp Gln Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr Thr 
            820                 825                 830         


Gly Asp Glu Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile Asp His 
        835                 840                 845             


Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val 
    850                 855                 860                 


Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser 
865                 870                 875                 880 


Leu Asp Ile Val Arg Ala Arg Lys Ala Glu Trp Val Arg Leu Tyr Lys 
                885                 890                 895     


Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu 
            900                 905                 910         


Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala Gly Phe Ile Lys Arg Gln 
        915                 920                 925             


Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp 
    930                 935                 940                 


Ala Arg Phe Asn Thr Glu His Asp Glu Asn Asp Lys Val Ile Arg Asp 
945                 950                 955                 960 


Val Lys Val Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys 
                965                 970                 975     


Asp Phe Glu Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala 
            980                 985                 990         


His Asp Ala Tyr Leu Asn Ala Val  Val Gly Thr Ala Leu  Leu Lys Lys 
        995                 1000                 1005             


Tyr Pro  Lys Leu Ala Ser Glu  Phe Val Tyr Gly Glu  Tyr Lys Lys 
    1010                 1015                 1020             


Tyr Asp  Ile Arg Lys Phe Ile  Thr Asn Ser Ser Asp  Lys Ala Thr 
    1025                 1030                 1035             


Ala Lys  Tyr Phe Phe Tyr Ser  Asn Leu Met Asn Phe  Phe Lys Thr 
    1040                 1045                 1050             


Lys Val  Lys Tyr Ala Asp Gly  Thr Val Phe Glu Arg  Pro Ile Ile 
    1055                 1060                 1065             


Glu Thr  Asn Ala Asp Gly Glu  Ile Ala Trp Asn Lys  Gln Ile Asp 
    1070                 1075                 1080             


Phe Glu  Lys Val Arg Lys Val  Leu Ser Tyr Pro Gln  Val Asn Ile 
    1085                 1090                 1095             


Val Lys  Lys Val Glu Thr Gln  Thr Gly Gly Phe Ser  Lys Glu Ser 
    1100                 1105                 1110             


Ile Leu  Pro Lys Gly Asp Ser  Asp Lys Leu Ile Pro  Arg Lys Thr 
    1115                 1120                 1125             


Lys Lys  Val Tyr Trp Asp Thr  Lys Lys Tyr Gly Gly  Phe Asp Ser 
    1130                 1135                 1140             


Pro Thr  Val Ala Tyr Ser Val  Phe Val Val Ala Asp  Val Glu Lys 
    1145                 1150                 1155             


Gly Lys  Ala Lys Lys Leu Lys  Thr Val Lys Glu Leu  Val Gly Ile 
    1160                 1165                 1170             


Ser Ile  Met Glu Arg Ser Phe  Phe Glu Glu Asn Pro  Val Glu Phe 
    1175                 1180                 1185             


Leu Glu  Asn Lys Gly Tyr His  Asn Ile Arg Glu Asp  Lys Leu Ile 
    1190                 1195                 1200             


Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Phe Glu Gly  Gly Arg Arg 
    1205                 1210                 1215             


Arg Leu  Leu Ala Ser Ala Ser  Glu Leu Gln Lys Gly  Asn Glu Met 
    1220                 1225                 1230             


Val Leu  Pro Gly Tyr Leu Val  Glu Leu Leu Tyr His  Ala His Arg 
    1235                 1240                 1245             


Ala Asp  Asn Phe Asn Ser Thr  Glu Tyr Leu Asn Tyr  Val Ser Glu 
    1250                 1255                 1260             


His Lys  Lys Glu Phe Glu Lys  Val Leu Ser Cys Val  Glu Asp Phe 
    1265                 1270                 1275             


Ala Asn  Leu Tyr Val Asp Val  Glu Lys Asn Leu Ser  Lys Ile Arg 
    1280                 1285                 1290             


Ala Val  Ala Asp Ser Met Asp  Asn Phe Ser Ile Glu  Glu Ile Ser 
    1295                 1300                 1305             


Asn Ser  Phe Ile Asn Leu Leu  Thr Leu Thr Ala Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Asp  Phe Asn Phe Leu Gly  Glu Lys Ile Pro Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Cys Leu  Thr Ala Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Lys Leu Gly 
    1355                 1360                 1365             


Glu Glu  
    1370 


<210>  1014
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1014

Met Lys Asn Ala Asn Lys Val Thr Gly Lys Tyr Tyr Leu Gly Phe Asp 
1               5                   10                  15      


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn 
            20                  25                  30          


Leu Cys Lys Phe Lys Lys Lys Ser Met Trp Gly Ile Arg Leu Phe Glu 
        35                  40                  45              


Asp Ala Lys Thr Ala Glu Glu Arg Arg Gly Gln Arg Ala Ser Arg Arg 
    50                  55                  60                  


Arg Leu Gln Arg Arg Lys Gln Arg Ile Asp Leu Leu Gln Glu Ile Phe 
65                  70                  75                  80  


Ala Glu Glu Ile Asn Lys Val Asp Glu Thr Phe Phe Ile Arg Leu Asn 
                85                  90                  95      


Glu Ser Arg Leu His Leu Asp Asp Lys Thr Val Ser Glu Lys His Pro 
            100                 105                 110         


Leu Phe Ile Gly Ser Glu Tyr Ser Glu Lys Glu Tyr Tyr Arg Glu Tyr 
        115                 120                 125             


Pro Thr Ile Phe His Leu Arg Lys Glu Leu Met Glu Ser Ser Glu Pro 
    130                 135                 140                 


His Asp Ile Arg Leu Val Tyr Leu Ala Ile His His Ile Leu Lys Asn 
145                 150                 155                 160 


Arg Gly His Phe Leu Ile Asp Gly Glu Leu Ser Lys Ala Asn Ser Phe 
                165                 170                 175     


Asp Ala Ala Phe Tyr Gln Met Lys Glu Val Leu Cys Asn Glu Leu Asp 
            180                 185                 190         


Leu Glu Ile Asp Gly Glu Asn Thr Thr Glu Val Glu Arg Thr Leu Arg 
        195                 200                 205             


Asp Arg Asn Ile Ser Lys Ser Asp Lys Val Lys Leu Leu Ala Ala Leu 
    210                 215                 220                 


Leu Ala Met Asn Gln Asn Glu Thr Glu Ser Lys Glu Thr Lys Lys Lys 
225                 230                 235                 240 


Ile Glu Asn Ile Cys Lys Leu Leu Ala Gly Asn Lys Gly Asp Ile Ser 
                245                 250                 255     


Lys Ile Phe Gly Gln Thr Pro Glu Gly Val Glu Lys Thr Ser Phe Ser 
            260                 265                 270         


Phe Gly Glu Ala Asp Tyr Asp Glu Ser Ile Arg Pro Asn Leu Glu Glu 
        275                 280                 285             


Ser Ile Pro Asp Gln Cys Phe Val Ile Asp Ser Ile Lys Ala Leu Tyr 
    290                 295                 300                 


Asp Trp Asn Val Leu Ala Asp Ile Leu Asn Gly Gln Thr Tyr Leu Ser 
305                 310                 315                 320 


Cys Ala Lys Val Asn Gln Tyr Glu Thr His Lys Asn Asn Leu Lys Glu 
                325                 330                 335     


Leu Lys Leu Leu Val Asn Arg Tyr Cys Ser Arg Glu Glu Tyr Lys Arg 
            340                 345                 350         


Phe Phe Lys Ser Ala Val Glu Lys Ala Asn Tyr Ser Asn Tyr Ile Gly 
        355                 360                 365             


Ser Ala Lys Gln Gly Asn Lys Ile Ile Asp Val Lys Arg Cys Ala Glu 
    370                 375                 380                 


Glu Asp Phe Tyr Lys Glu Leu Lys Ile Leu Leu Ser Lys Ile Glu Pro 
385                 390                 395                 400 


Glu Glu Ala Asp Arg Glu Leu Tyr Glu Arg Ile Lys Arg Glu Ala Glu 
                405                 410                 415     


Glu Gly Ser Leu Leu Pro Leu Gln Arg Asn Lys Asp Asn Ser Val Val 
            420                 425                 430         


Pro Asn Gln Met His Lys Ala Glu Leu Ala Leu Ile Leu Glu Asn Ala 
        435                 440                 445             


Gly Ser Tyr Leu Gly Phe Leu Asn Glu Lys Asp Glu Asp Gly Leu Ser 
    450                 455                 460                 


Pro Ala Glu Lys Ile Ile Glu Leu Phe Ser Phe Arg Ile Pro Tyr Tyr 
465                 470                 475                 480 


Val Gly Pro Leu Ser Asp Arg His Lys Asp Arg Gly Ser Asn Ala Trp 
                485                 490                 495     


Ile Val Arg Lys Ala Glu Gly Arg Ile Leu Pro Trp Asn Phe Asp Glu 
            500                 505                 510         


Lys Val Asp Arg Glu Lys Ser Asn Glu Ala Phe Ile Glu Arg Met Thr 
        515                 520                 525             


Asn Lys Cys Thr Tyr Leu Leu Gly Lys Asp Val Val Ala Lys Asn Ser 
    530                 535                 540                 


Leu Ile Tyr Ser His Phe Met Val Leu Asn Glu Leu Asn Asn Leu Ser 
545                 550                 555                 560 


Val Cys Gly Asn Lys Ile Ser Val Gln Val Lys Gln Asp Ile Tyr Arg 
                565                 570                 575     


Glu Leu Phe Gln Arg His Thr Lys Val Ser Lys Lys Met Leu Leu Gln 
            580                 585                 590         


Tyr Cys Arg Glu Lys Met Met Asn Lys Asp Leu Glu Glu Glu Asp Leu 
        595                 600                 605             


Ser Gly Phe Asp Gly Asp Phe Lys Ala Lys Leu Ser Ser Tyr Leu Asp 
    610                 615                 620                 


Phe Glu Lys Lys Val Phe Gly Ala Lys Met Glu Glu Asp Arg Ile Arg 
625                 630                 635                 640 


Lys Ile Thr Glu Asp Ile Ile Arg Trp Lys Thr Ile Tyr Gly Asp Asp 
                645                 650                 655     


Gly Lys Met Leu Gln Ala Val Val Glu Lys Glu Tyr Pro Gly Glu Leu 
            660                 665                 670         


Asp Ser Asp Gln Leu Lys Ala Val Lys Gln Phe Arg Tyr Ser Gly Trp 
        675                 680                 685             


Gly Asn Phe Ser Glu Glu Phe Leu Asn Gly Ile Val Gly Thr Glu Lys 
    690                 695                 700                 


Pro Thr Gly Gln Met Phe Thr Ile Ile Glu Ala Leu Trp Gln Thr Asn 
705                 710                 715                 720 


Cys Asn Leu Met Gln Leu Leu Ser Lys Asn Phe Thr Phe Met Glu Glu 
                725                 730                 735     


Ile Glu Lys His Asn Arg Glu Leu Ala Gly Gln Ile Thr Asp Ile Ser 
            740                 745                 750         


Tyr Asp Lys Leu Val Lys Asp Leu Tyr Ile Ser Pro Ala Asn Lys Arg 
        755                 760                 765             


Ala Val Trp Gln Thr Val Arg Ile Ala Glu Glu Val Lys Lys Ile Met 
    770                 775                 780                 


Gly Cys Glu Pro Ala Lys Ile Phe Val Glu Met Ala Arg Gly Gly Glu 
785                 790                 795                 800 


Lys Glu Lys Lys Arg Thr Ile Ser Arg Lys Lys Lys Leu Leu Glu Leu 
                805                 810                 815     


Tyr Ala Ala Cys Lys Lys Asp Ala Arg Asp Trp Asn Arg Glu Ile Glu 
            820                 825                 830         


Asp Arg Asp Glu Arg Glu Phe Asn Ser Asp Lys Leu Tyr Leu Tyr Tyr 
        835                 840                 845             


Thr Gln Met Gly Arg Cys Met Tyr Ser Gly Glu Arg Ile Asp Leu His 
    850                 855                 860                 


Glu Leu Met Ser Ala Asn Ser Lys Trp Asp Ile Asp His Ile Tyr Pro 
865                 870                 875                 880 


Gln Ser Lys Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Ser Leu 
                885                 890                 895     


Lys Lys Ile Asn Asn Glu Lys Gly Asn Lys Pro Leu Ser Arg Asp Ile 
            900                 905                 910         


Gln Gln Ser Gln Gln Lys Trp Trp Thr Glu Leu Leu Gln Leu Glu Phe 
        915                 920                 925             


Ile Thr Lys Lys Lys Tyr Asp Arg Leu Met His Arg Gly Glu Leu Ser 
    930                 935                 940                 


Glu Glu Glu Leu Ser Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg 
945                 950                 955                 960 


Gln Ser Thr Lys Ala Ala Ala Glu Leu Leu Gly Arg Met Tyr Asn Asn 
                965                 970                 975     


Ser Lys Val Val Tyr Val Lys Ala Pro Leu Val Ser Gln Phe Arg Lys 
            980                 985                 990         


Asp Asp Leu Asn Ile Leu Lys Ser  Arg Leu Ala Asn Asp  Tyr His His 
        995                 1000                 1005             


Ala Lys  Asp Ala Tyr Leu Asn  Ile Ala Val Gly Asn  Val Tyr Asn 
    1010                 1015                 1020             


Glu Lys  Phe Thr Asp Asn Pro  Leu Arg Trp Ile Glu  Lys His Ala 
    1025                 1030                 1035             


Gly Glu  Asn Tyr Ser Ile Asn  Arg Val Phe Asp Tyr  Asp Ile Val 
    1040                 1045                 1050             


Ala Gly  Asp Gly Ala Pro Val  Trp Ala Ala Pro Leu  Arg Asp Glu 
    1055                 1060                 1065             


Lys Asn  Lys Leu Arg Lys Asn  Glu Val Gly Glu Lys  Tyr Gly Gly 
    1070                 1075                 1080             


Thr Ile  Asp Val Ile Arg Lys  Thr Val Lys Arg Asn  Asp Val Leu 
    1085                 1090                 1095             


Tyr Thr  Glu His Thr Tyr Cys  Gly Lys Gly Glu Leu  Phe Asn Ala 
    1100                 1105                 1110             


Thr Ile  Ala Lys Lys Asp Gly  Gly Ala Glu Ile Pro  Leu Lys Ala 
    1115                 1120                 1125             


Gly Leu  Gln Thr Gly Lys Tyr  Gly Gly Tyr Lys Ser  Pro Asn Thr 
    1130                 1135                 1140             


Ser Tyr  Phe Ala Ala Ile Glu  Phe Asp Gly Lys Lys  Gly Glu Arg 
    1145                 1150                 1155             


Val Lys  Asn Ile Met Glu Val  Pro Val Tyr Ile Ala  Asn Ile Leu 
    1160                 1165                 1170             


Leu His  Asp Ile Asp Ala Tyr  Ile Arg Tyr Cys Glu  Thr Val Lys 
    1175                 1180                 1185             


Gly Leu  Lys Asn Val Lys Ile  Leu Arg Pro Cys Ile  Lys Lys Asn 
    1190                 1195                 1200             


Ala Leu  Ile Ser Val Asp Gly  Tyr Pro Met Arg Ile  Arg Gly Ala 
    1205                 1210                 1215             


Asn Glu  Lys Asn Ile Leu Leu  Lys Asn Asn Leu Gln  Pro Ile Phe 
    1220                 1225                 1230             


Gly Arg  His Glu Glu Thr Val  Arg Arg Ile Glu Lys  Tyr Val Glu 
    1235                 1240                 1245             


Lys Asn  Gln Glu Phe Glu Val  Asp Glu Lys Phe Asp  Gly Leu Asn 
    1250                 1255                 1260             


Glu Ala  Asn Leu Thr Asp Leu  Tyr Asp Asp Ile Thr  Glu Lys Leu 
    1265                 1270                 1275             


Cys Gly  Pro Tyr Val Glu Arg  Pro Ser Asn Gln Gly  Glu Tyr Leu 
    1280                 1285                 1290             


Met Glu  Asn Arg Glu Lys Phe  Leu Asn Ile Ser Leu  Lys Glu Lys 
    1295                 1300                 1305             


Ala Lys  Leu Leu Ala Gln Met  Ile Lys Leu Val Arg  Cys Asp Thr 
    1310                 1315                 1320             


Glu Thr  Lys Ala Asp Leu Arg  Ser Ile Gly Ala Ser  Lys Asn Ala 
    1325                 1330                 1335             


Gly Val  Ile Ser Val Asn Lys  Asn Thr Leu Gly Gly  Lys Arg Leu 
    1340                 1345                 1350             


Ile Leu  Ile Asn Gln Ser Val  Thr Gly Leu Phe Glu  Asn Arg Ile 
    1355                 1360                 1365             


Glu Leu  
    1370 


<210>  1015
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1015

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Tyr Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Arg Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Lys Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Arg Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Leu Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Arg Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Leu Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Phe Leu Ala Glu Gly Phe Lys Asp Phe Gln Phe Leu Asn Arg Lys 
    530                 535                 540                 


Gln Lys Glu Thr Ile Phe Asn Ser Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Ser Phe Leu Asn Lys Val Asp Gly Tyr Glu 
                565                 570                 575     


Gly Ile Ala Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Gly Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Thr Asp Asn Glu Leu Ile Leu Glu Asp Ile Val Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Asp Ile Tyr Lys Asp 
625                 630                 635                 640 


Phe Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Ala Gly Leu Ser Phe Lys 
    690                 695                 700                 


Pro Ile Ile Asp Lys Ala Arg Thr Gly Ser His Ser Asp Asn Leu Lys 
705                 710                 715                 720 


Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Leu Ser Arg Ser Arg Gln Arg Leu Thr Thr Leu Arg Glu 
    770                 775                 780                 


Ser Leu Ala Asn Leu Lys Ser Asn Ile Leu Glu Glu Lys Lys Pro Lys 
785                 790                 795                 800 


Tyr Val Lys Asp Gln Val Glu Asn His His Leu Ser Asp Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Val Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Asp Asp Lys  Leu Met Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Leu Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Gln  Lys Gln Glu Phe Val  Val Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Ile Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Asn Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Pro Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1016
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1016

Met Thr Lys Ile Lys Asp Asp Tyr Ile Val Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asp Ser Cys Gly Trp Val Ala Met Asn Ser Asn Asn Asp Ile Leu Lys 
            20                  25                  30          


Leu Gln Gly Lys Thr Ala Ile Gly Ser Arg Leu Phe Glu Gly Gly Lys 
        35                  40                  45              


Ser Ala Ala Glu Arg Arg Leu Phe Arg Thr Thr His Arg Arg Ile Lys 
    50                  55                  60                  


Arg Arg Arg Trp Arg Leu Lys Leu Leu Glu Glu Phe Phe Asp Pro Tyr 
65                  70                  75                  80  


Met Ala Glu Val Asp Pro Tyr Phe Phe Ala Arg Leu Lys Glu Ser Gly 
                85                  90                  95      


Leu Ser Pro Leu Asp Lys Arg Lys Thr Val Ser Ser Ile Val Phe Pro 
            100                 105                 110         


Thr Ser Ala Glu Asp Lys Lys Phe Tyr Asp Asp Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Tyr Lys Leu Met Thr Glu Asp Glu Lys Phe Asp Leu Arg 
    130                 135                 140                 


Glu Val Tyr Leu Ala Ile His His Ile Ile Lys Tyr Arg Gly Asn Phe 
145                 150                 155                 160 


Leu Tyr Asn Thr Ser Val Lys Asp Phe Lys Ala Ser Lys Ile Asp Val 
                165                 170                 175     


Lys Ser Ser Ile Glu Lys Leu Asn Glu Leu Tyr Glu Asn Leu Gly Leu 
            180                 185                 190         


Asp Leu Asn Val Glu Phe Asn Ile Ser Asn Thr Ala Glu Ile Glu Lys 
        195                 200                 205             


Val Leu Lys Asp Lys Gln Ile Phe Lys Arg Asp Lys Val Lys Lys Ile 
    210                 215                 220                 


Ala Glu Leu Phe Ala Ile Lys Thr Asp Asn Lys Glu Gln Ser Lys Arg 
225                 230                 235                 240 


Ile Lys Asp Ile Ser Lys Gln Val Ala Asn Ala Val Leu Gly Tyr Lys 
                245                 250                 255     


Thr Arg Phe Asp Thr Ile Ala Leu Lys Glu Ile Ser Lys Asp Glu Leu 
            260                 265                 270         


Ser Asp Trp Asn Phe Lys Leu Ser Asp Ile Asp Ala Asp Ser Lys Phe 
        275                 280                 285             


Glu Ala Leu Met Gly Asn Leu Asp Glu Asn Glu Gln Ala Ile Leu Leu 
    290                 295                 300                 


Thr Ile Lys Glu Leu Phe Asn Glu Val Thr Leu Asn Gly Ile Val Glu 
305                 310                 315                 320 


Asp Gly Asn Thr Leu Ser Glu Ser Met Ile Asn Lys Tyr Asn Asp His 
                325                 330                 335     


Arg Asp Asp Leu Lys Leu Leu Lys Glu Val Ile Glu Asn His Ile Asp 
            340                 345                 350         


Arg Lys Lys Ala Lys Glu Leu Ala Leu Ala Tyr Asp Leu Tyr Val Asn 
        355                 360                 365             


Asn Arg His Gly Arg Leu Leu Gln Ala Lys Lys Lys Leu Gly Lys Ile 
    370                 375                 380                 


Lys Pro Arg Ser Lys Glu Asp Phe Tyr Lys Val Val Asn Lys Asn Leu 
385                 390                 395                 400 


Asp Asp Ser Arg Ala Ser Lys Glu Ile Lys Lys Lys Ile Glu Leu Asp 
                405                 410                 415     


Ser Phe Met Pro Lys Gln Arg Thr Asn Ala Asn Gly Val Ile Pro Tyr 
            420                 425                 430         


Gln Leu Gln Gln Leu Glu Leu Asp Lys Ile Ile Glu Asn Gln Ser Lys 
        435                 440                 445             


Tyr Tyr Pro Phe Leu Lys Glu Ile Asn Pro Val Ser Ser His Leu Lys 
    450                 455                 460                 


Glu Ala Pro Tyr Lys Leu Asp Glu Leu Ile Arg Phe Arg Val Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ile Ser Pro Asn Glu Ser Thr Lys Asp Ile Gln 
                485                 490                 495     


Thr Lys Lys Asn Gln Asn Phe Ala Trp Met Ile Arg Lys Glu Glu Gly 
            500                 505                 510         


Arg Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Ile Glu Ser 
        515                 520                 525             


Ala Asn Lys Phe Ile Lys Arg Met Thr Thr Lys Asp Thr Tyr Leu Phe 
    530                 535                 540                 


Gly Glu Asp Val Leu Pro Ala Asn Ser Leu Leu Tyr Gln Lys Phe Thr 
545                 550                 555                 560 


Val Leu Asn Glu Leu Asn Asn Ile Arg Ile Asn Gly Lys Arg Ile Ser 
                565                 570                 575     


Val Asp Leu Lys Gln Glu Ile Tyr Glu Asn Leu Phe Lys Lys His Thr 
            580                 585                 590         


Thr Val Thr Val Lys Lys Leu Glu Asn Tyr Leu Lys Glu Asn His Asn 
        595                 600                 605             


Leu Val Lys Val Glu Ile Lys Gly Leu Ala Asp Glu Lys Lys Phe Asn 
    610                 615                 620                 


Ser Gly Leu Thr Thr Tyr Asn Arg Phe Lys Asn Leu Asn Ile Phe Asp 
625                 630                 635                 640 


Asn Gln Ile Asp Asp Leu Lys Tyr Arg Asn Asp Phe Glu Lys Ile Ile 
                645                 650                 655     


Glu Trp Ser Thr Ile Phe Glu Asp Lys Ser Ile Tyr Lys Glu Lys Leu 
            660                 665                 670         


Arg Ser Ile Asp Trp Leu Asn Glu Lys Gln Ile Asn Ala Leu Ser Asn 
        675                 680                 685             


Ile Arg Leu Gln Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Ala Gln 
    690                 695                 700                 


Leu His Asp His Asn Gly Gln Thr Ile Ile Glu Gln Leu Trp Asp Ser 
705                 710                 715                 720 


Gln Asn Asn Phe Met Gln Ile Val Thr Gln Ala Asp Phe Lys Asp Ala 
                725                 730                 735     


Ile Ala Lys Ala Asn Gln Asn Leu Leu Val Ala Thr Ser Val Glu Asp 
            740                 745                 750         


Ile Leu Asn Asn Ala Tyr Thr Ser Pro Ala Asn Lys Lys Ala Ile Arg 
        755                 760                 765             


Gln Val Ile Lys Val Val Asp Asp Ile Val Lys Ala Ala Ser Gly Lys 
    770                 775                 780                 


Val Pro Lys Gln Ile Ala Ile Glu Phe Thr Arg Asp Ala Asp Glu Asn 
785                 790                 795                 800 


Pro Lys Arg Ser Gln Thr Arg Gly Ser Lys Leu Gln Lys Val Tyr Lys 
                805                 810                 815     


Asp Leu Ser Thr Glu Leu Ala Ser Lys Thr Ile Ala Glu Glu Leu Asn 
            820                 825                 830         


Glu Ala Ile Lys Asp Lys Lys Leu Val Gln Asp Lys Tyr Tyr Leu Tyr 
        835                 840                 845             


Phe Met Gln Leu Gly Arg Asp Ala Tyr Thr Gly Glu Pro Ile Asn Ile 
    850                 855                 860                 


Asp Glu Ile Gln Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe 
865                 870                 875                 880 


Ile Lys Asp Asp Ala Leu Asp Asn Arg Val Leu Val Ser Arg Ala Val 
                885                 890                 895     


Asn Asn Gly Lys Ser Asp Asn Val Pro Val Lys Leu Phe Gly Asn Glu 
            900                 905                 910         


Met Ala Ala Asn Leu Gly Met Thr Ile Arg Lys Met Trp Glu Glu Trp 
        915                 920                 925             


Lys Asn Ile Gly Leu Ile Ser Lys Thr Lys Tyr Asn Asn Leu Leu Thr 
    930                 935                 940                 


Asp Pro Asp His Ile Asn Lys Tyr Lys Ser Ala Gly Phe Ile Arg Arg 
945                 950                 955                 960 


Gln Leu Val Glu Thr Ser Gln Ile Ile Lys Leu Val Ser Thr Ile Leu 
                965                 970                 975     


Gln Ser Arg Tyr Pro Asn Thr Glu Ile Ile Thr Val Lys Ala Lys Tyr 
            980                 985                 990         


Asn His Tyr Leu Arg Glu Lys Phe  Asp Leu Tyr Lys Ser  Arg Glu Val 
        995                 1000                 1005             


Asn Asp  Tyr His His Ala Ile  Asp Ala Tyr Leu Ser  Ala Ile Cys 
    1010                 1015                 1020             


Gly Asn  Leu Leu Tyr Gln Asn  Tyr Pro Asn Leu Arg  Pro Phe Phe 
    1025                 1030                 1035             


Val Tyr  Gly Gln Tyr Lys Lys  Phe Ser Ser Asp Pro  Asp Lys Glu 
    1040                 1045                 1050             


Lys Glu  Ile Phe Asn Lys Thr  Arg Lys Phe Ser Phe  Ile Ser Gln 
    1055                 1060                 1065             


Ile Leu  Lys Asn Lys Ser Glu  Asn Ser Lys Glu Ile  Ala Glu Lys 
    1070                 1075                 1080             


Leu Lys  Arg Ala Tyr Gln Phe  Lys Tyr Met Leu Val  Ser Arg Glu 
    1085                 1090                 1095             


Thr Glu  Thr Lys Asp Gln Glu  Met Phe Lys Met Thr  Val Phe Pro 
    1100                 1105                 1110             


Arg Glu  Thr Pro Gly Asn Leu  Ile Pro Lys Lys Lys  Gly Met Ala 
    1115                 1120                 1125             


Pro Glu  Ile Tyr Gly Gly Tyr  Thr Lys Asn Ser Asp  Ala Tyr Met 
    1130                 1135                 1140             


Val Ile  Val Lys Ile Asp Lys  Lys Lys Glu Thr Glu  Tyr Arg Ile 
    1145                 1150                 1155             


Leu Gly  Ile Pro Thr Arg Glu  Leu Val Asn Leu Lys  Lys Ala Glu 
    1160                 1165                 1170             


Lys Glu  Asp His Tyr Glu Ser  Tyr Leu Lys Glu Ile  Leu Ala Pro 
    1175                 1180                 1185             


Arg Ile  Leu Tyr Asn Lys Asn  Gly Lys Arg Asp Lys  Lys Ile Thr 
    1190                 1195                 1200             


Ser Phe  Glu Ile Val Lys Ser  Lys Ile Pro Tyr Lys  Gln Val Ile 
    1205                 1210                 1215             


Gln Asp  Gly Asp Lys Lys Phe  Met Leu Gly Ser Ser  Thr Tyr Val 
    1220                 1225                 1230             


Tyr Asn  Ala Lys Gln Leu Thr  Leu Ser Thr Glu Ser  Met Lys Ala 
    1235                 1240                 1245             


Ile Thr  Asn Asn Phe Asp Lys  Asp Ser Asp Glu Asn  Asp Ala Leu 
    1250                 1255                 1260             


Ile Lys  Ala Tyr Asp Glu Ile  Leu Asp Lys Val Asp  Lys Tyr Leu 
    1265                 1270                 1275             


Pro Leu  Phe Asp Ile Asn Ala  Phe Arg Lys Lys Leu  His Ser Gly 
    1280                 1285                 1290             


Arg Glu  Lys Phe Val Glu Leu  Ser Leu Glu Asp Lys  Lys Asp Ile 
    1295                 1300                 1305             


Ile Leu  Lys Val Leu Ile Gly  Leu His Asp Asn Pro  Ala Thr Ser 
    1310                 1315                 1320             


Asp Leu  Lys Thr Leu Gly Phe  Ser Ser Thr Glu Leu  Gly Lys Met 
    1325                 1330                 1335             


Gln Lys  Asn Ser Gly Ile Val  Leu Ser Gly Asn Ala  Lys Leu Ile 
    1340                 1345                 1350             


Tyr Gln  Ser Pro Thr Gly Leu  Phe Lys Lys Ser Val  Lys Ile Ser 
    1355                 1360                 1365             


Asp Leu  
    1370 


<210>  1017
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1017

Met Thr Lys Ile Lys Asp Asp Tyr Ile Val Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asp Ser Cys Gly Trp Val Ala Met Asn Ser Asp Asn Asp Ile Leu Lys 
            20                  25                  30          


Leu Gln Gly Lys Thr Ala Ile Gly Ser His Leu Phe Glu Ala Gly Lys 
        35                  40                  45              


Ser Ala Ala Asp Arg Arg Leu Phe Arg Thr Thr His Arg Arg Ile Lys 
    50                  55                  60                  


Arg Arg Arg Trp Arg Leu Lys Leu Leu Glu Glu Phe Phe Asp Pro Tyr 
65                  70                  75                  80  


Met Ala Lys Val Asp Pro Tyr Phe Phe Ala Arg Leu Lys Glu Ser Gly 
                85                  90                  95      


Leu Ser Pro Leu Asp Lys Arg Lys Asn Val Phe Ser Ile Val Phe Pro 
            100                 105                 110         


Thr Ala Ala Glu Asp Lys Lys Phe Tyr Asp Asp Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Tyr Lys Leu Met Thr Glu Asp Glu Lys Phe Asp Leu Arg 
    130                 135                 140                 


Glu Val Tyr Leu Ala Ile His His Ile Ile Lys Tyr Arg Gly Asn Phe 
145                 150                 155                 160 


Leu Tyr Asn Thr Ser Val Lys Asp Phe Lys Ala Ser Lys Ile Asp Val 
                165                 170                 175     


Lys Ser Ser Ile Glu Lys Leu Asn Glu Leu Tyr Glu Asn Leu Gly Leu 
            180                 185                 190         


Asp Leu Asn Val Glu Phe Asn Ile Ser Asn Thr Ala Glu Ile Glu Lys 
        195                 200                 205             


Val Leu Lys Asp Lys Gln Ile Phe Lys Arg Asp Lys Val Lys Lys Ile 
    210                 215                 220                 


Ala Glu Leu Phe Ala Ile Lys Thr Asp Asn Lys Glu Gln Ser Lys Arg 
225                 230                 235                 240 


Ile Lys Asp Ile Ser Lys Gln Val Ala Asn Ala Val Leu Gly Tyr Lys 
                245                 250                 255     


Thr Arg Phe Asp Thr Ile Ala Leu Lys Glu Val Ser Lys Asp Glu Leu 
            260                 265                 270         


Ser Asp Trp Asn Phe Lys Leu Ser Asp Ile Asp Ala Asp Ser Lys Phe 
        275                 280                 285             


Glu Ala Leu Met Gly Asn Leu Asp Glu Asn Glu Gln Ala Ile Leu Leu 
    290                 295                 300                 


Thr Ile Lys Glu Leu Phe Asn Ala Val Thr Leu Asn Gly Ile Val Glu 
305                 310                 315                 320 


Asp Gly Asn Thr Leu Ser Glu Ser Met Ile Asn Lys Tyr Asn Asp His 
                325                 330                 335     


Arg Asp Asp Leu Lys Leu Leu Lys Glu Val Ile Glu Asn His Ile Asp 
            340                 345                 350         


Arg Lys Lys Ala Val Lys Leu Ala Leu Ala Tyr Asp Leu Tyr Val Asn 
        355                 360                 365             


Asn Arg His Gly Gln Leu Ile Gln Ala Lys Lys Lys Leu Gly Lys Ile 
    370                 375                 380                 


Lys Pro Gly Ser Lys Glu Asp Phe Tyr Lys Val Val Asn Lys Asn Leu 
385                 390                 395                 400 


Asp Asp Ser Gln Ala Ser Lys Glu Ile Lys Lys Lys Ile Glu Leu Asp 
                405                 410                 415     


Ser Phe Met Pro Lys Gln Arg Thr Asn Ala Asn Gly Val Ile Pro Tyr 
            420                 425                 430         


Gln Leu His Gln Leu Glu Leu Asp Lys Ile Ile Glu Asn Gln Ser Lys 
        435                 440                 445             


Tyr Tyr Pro Phe Leu Lys Glu Ile Asn Pro Val Ser Ser His Leu Lys 
    450                 455                 460                 


Glu Ala Pro Tyr Lys Leu Asp Glu Leu Ile Arg Phe Arg Val Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ile Ser Pro Asn Glu Ser Thr Lys Asp Ile Gln 
                485                 490                 495     


Thr Lys Lys Asn Gln Asn Phe Ala Trp Met Ile Arg Lys Glu Glu Gly 
            500                 505                 510         


Arg Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Ile Glu Ser 
        515                 520                 525             


Ala Asn Lys Phe Ile Lys Arg Met Thr Thr Lys Asp Thr Tyr Leu Phe 
    530                 535                 540                 


Gly Glu Asn Val Leu Pro Ala Asn Ser Leu Leu Tyr Gln Lys Phe Thr 
545                 550                 555                 560 


Val Leu Asn Glu Leu Asn Asn Ile Arg Ile Asn Gly Lys Arg Ile Ser 
                565                 570                 575     


Val Asp Leu Lys Gln Glu Ile Tyr Glu Asn Leu Phe Lys Lys His Thr 
            580                 585                 590         


Thr Val Thr Val Lys Lys Leu Glu Asn Tyr Leu Lys Gln Asn His Asn 
        595                 600                 605             


Leu Val Lys Val Glu Ile Lys Gly Leu Ala Asp Glu Lys Lys Phe Asn 
    610                 615                 620                 


Ser Gly Leu Thr Thr Tyr Asn Arg Phe Lys Asn Leu Asn Ile Phe Asp 
625                 630                 635                 640 


Asn Gln Ile Asp Asp Leu Lys Tyr Arg Lys Asp Phe Glu Lys Ile Ile 
                645                 650                 655     


Glu Trp Ser Thr Ile Phe Glu Asp Lys Ser Ile Tyr Lys Glu Lys Leu 
            660                 665                 670         


Arg Ser Ile Asp Trp Leu Asn Glu Lys Gln Ile Asn Ala Leu Ser Asn 
        675                 680                 685             


Ile Arg Leu Gln Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Ala Gln 
    690                 695                 700                 


Leu His Asp His Asn Gly Gln Thr Ile Ile Glu Gln Leu Trp Asp Ser 
705                 710                 715                 720 


Gln Asn Asn Phe Met Gln Ile Val Thr Gln Ala Asp Phe Lys Asp Ala 
                725                 730                 735     


Ile Ala Lys Ala Asn Gln Asn Leu Leu Val Ala Thr Ser Val Glu Asp 
            740                 745                 750         


Ile Leu Asn Asp Ala Tyr Thr Ser Pro Ala Asn Lys Lys Ala Ile Arg 
        755                 760                 765             


Gln Val Ile Lys Val Val Asp Asp Ile Val Lys Ala Ala Ser Gly Lys 
    770                 775                 780                 


Val Pro Lys Gln Ile Ala Ile Glu Phe Thr Arg Asp Ala Asp Asp Asn 
785                 790                 795                 800 


Pro Lys Arg Ser Gln Thr Arg Gly Ser Lys Leu Gln Lys Val Tyr Lys 
                805                 810                 815     


Asp Leu Ser Thr Glu Leu Ala Ser Lys Ala Ile Ala Glu Glu Leu Asn 
            820                 825                 830         


Glu Ala Ile Lys Asp Lys Lys Leu Val Gln Asp Lys Tyr Tyr Leu Tyr 
        835                 840                 845             


Phe Met Gln Leu Gly Arg Asp Ala Tyr Thr Gly Glu Pro Ile Asn Ile 
    850                 855                 860                 


Asp Glu Ile Gln Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe 
865                 870                 875                 880 


Ile Lys Asp Asp Ala Leu Asp Asn Arg Val Leu Val Ser Lys Ala Val 
                885                 890                 895     


Asn Asn Asp Lys Ser Asp Asn Val Pro Val Lys Leu Phe Gly Asn Glu 
            900                 905                 910         


Met Ala Gly Asn Leu Gly Ile Thr Ile Arg Lys Met Trp Glu Glu Trp 
        915                 920                 925             


Lys Asn Ile Gly Leu Ile Ser Lys Thr Lys Tyr Asn Asn Leu Leu Thr 
    930                 935                 940                 


Asp Pro Asp His Ile Asn Lys Tyr Glu Ser Ala Gly Phe Ile Arg Arg 
945                 950                 955                 960 


Gln Leu Val Glu Thr Ser Gln Ile Ile Lys Leu Val Ser Thr Ile Leu 
                965                 970                 975     


Gln Ser Arg Tyr Pro Asn Thr Glu Ile Ile Thr Val Lys Ala Lys Tyr 
            980                 985                 990         


Asn His Tyr Leu Arg Glu Lys Phe  Asp Leu Tyr Lys Ser  Arg Glu Val 
        995                 1000                 1005             


Asn Asp  Tyr His His Ala Ile  Asp Ala Tyr Leu Ser  Ala Ile Cys 
    1010                 1015                 1020             


Gly Asn  Leu Leu Tyr Gln Asn  Tyr Pro Asn Leu Arg  Pro Phe Phe 
    1025                 1030                 1035             


Val Tyr  Gly Gln Tyr Lys Lys  Phe Ser Ser Asp Pro  Asp Lys Glu 
    1040                 1045                 1050             


Lys Glu  Ile Phe Asn Lys Thr  Arg Lys Phe Ser Phe  Ile Ser Gln 
    1055                 1060                 1065             


Ile Leu  Lys Asn Lys Ser Glu  Asn Ser Lys Glu Ile  Ala Glu Lys 
    1070                 1075                 1080             


Leu Lys  Arg Ala Tyr Gln Phe  Lys Tyr Met Leu Val  Ser Arg Glu 
    1085                 1090                 1095             


Thr Glu  Thr Lys Asp Gln Glu  Met Phe Lys Met Thr  Val Phe Pro 
    1100                 1105                 1110             


Arg Glu  Thr Pro Gly Asn Leu  Ile Pro Lys Lys Lys  Gly Met Ala 
    1115                 1120                 1125             


Thr Glu  Ile Tyr Gly Gly Tyr  Thr Lys Asn Ser Asp  Ala Tyr Met 
    1130                 1135                 1140             


Val Ile  Val Lys Ile Asp Lys  Lys Lys Glu Thr Glu  Tyr Arg Ile 
    1145                 1150                 1155             


Leu Gly  Ile Pro Thr Arg Glu  Leu Val Asn Leu Lys  Lys Ala Glu 
    1160                 1165                 1170             


Lys Glu  Asp His Tyr Glu Ser  Tyr Leu Lys Glu Ile  Leu Ala Pro 
    1175                 1180                 1185             


Arg Ile  Leu Tyr Asn Lys Asn  Gly Lys Arg Asp Lys  Lys Ile Thr 
    1190                 1195                 1200             


Ser Phe  Glu Ile Val Lys Ser  Lys Ile Pro Tyr Lys  Gln Val Ile 
    1205                 1210                 1215             


Gln Asp  Gly Asp Lys Lys Phe  Met Leu Gly Ser Ser  Thr Tyr Val 
    1220                 1225                 1230             


Tyr Asn  Ala Lys Gln Leu Thr  Leu Ser Thr Glu Ser  Met Lys Ala 
    1235                 1240                 1245             


Ile Thr  Asn Asn Phe Asp Lys  Asp Ser Asp Glu Asn  Asp Ala Leu 
    1250                 1255                 1260             


Ile Lys  Ala Tyr Asp Glu Ile  Leu Asp Lys Val Asp  Lys Tyr Leu 
    1265                 1270                 1275             


Pro Leu  Phe Asp Ile Asn Ala  Phe Arg Lys Lys Leu  His Ser Gly 
    1280                 1285                 1290             


Arg Glu  Lys Phe Val Glu Leu  Ser Leu Glu Asp Lys  Lys Asp Ile 
    1295                 1300                 1305             


Ile Leu  Lys Val Leu Ile Gly  Leu His Asp Asn Pro  Ala Thr Ser 
    1310                 1315                 1320             


Asp Leu  Lys Thr Leu Gly Phe  Ser Ser Thr Glu Leu  Gly Lys Met 
    1325                 1330                 1335             


Gln Lys  Asn Ser Gly Ile Val  Leu Ser Gly Asn Ala  Lys Leu Ile 
    1340                 1345                 1350             


Tyr Gln  Ser Pro Thr Gly Leu  Phe Lys Lys Ser Val  Lys Ile Ser 
    1355                 1360                 1365             


Asp Leu  
    1370 


<210>  1018
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1018

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Tyr Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Arg Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Lys Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Arg Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Leu Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Arg Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Leu Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Phe Leu Ala Glu Gly Phe Lys Asp Phe Gln Phe Leu Asn Arg Lys 
    530                 535                 540                 


Gln Lys Glu Thr Ile Phe Asn Ser Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Ser Phe Leu Asn Lys Val Asp Gly Tyr Glu 
                565                 570                 575     


Gly Ile Ala Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Gly Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Thr Asp Asn Glu Leu Ile Leu Glu Asp Ile Val Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Asp Ile Tyr Lys Asp 
625                 630                 635                 640 


Phe Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Ala Gly Leu Ser Phe Lys 
    690                 695                 700                 


Pro Ile Ile Asp Lys Ala Arg Thr Gly Ser His Ser Asp Asn Leu Lys 
705                 710                 715                 720 


Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Leu Ser Arg Ser Arg Gln Arg Leu Thr Thr Leu Arg Glu 
    770                 775                 780                 


Ser Leu Ala Asn Leu Lys Ser Asn Ile Leu Glu Glu Lys Lys Pro Lys 
785                 790                 795                 800 


Tyr Val Lys Asp Gln Val Glu Asn His His Leu Ser Asp Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Val Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Lys Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Asp Asp Lys  Leu Met Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Leu Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Gln  Lys Gln Glu Phe Val  Val Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Ile Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Asn Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Pro Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Lys Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1019
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1019

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Gln Glu Glu Lys Asp Tyr 
        115                 120                 125             


His Glu Lys Phe Ser Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Ile Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Ser Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Ser Lys Gln Tyr Gln Asp Phe Leu Glu Ile 
            180                 185                 190         


Phe Asn Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Gln Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Ile Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Asp Ser Glu Asn Phe Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Lys Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Ile Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Phe Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Lys Asn Glu Gln Gly Glu Thr Tyr Phe Phe Asp Ser Asn Ile 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Glu His Arg Lys Val Ser 
545                 550                 555                 560 


Lys Lys Lys Leu Leu Asp Phe Leu Ala Lys Glu Tyr Glu Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Val Ile Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Glu Lys Ile Leu Asp Lys Asp Phe 
        595                 600                 605             


Leu Asp Asn Pro Asp Asn Glu Ser Ile Leu Glu Asp Ile Val Gln Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Asn 
625                 630                 635                 640 


Tyr Lys Asp Leu Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Glu Ser Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Arg Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu 
    690                 695                 700                 


Ser Phe Lys Ser Ile Ile Ser Lys Ala Gln Ala Gly Ser His Ser Asp 
705                 710                 715                 720 


Asn Leu Lys Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly Tyr Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Asn Gln Gly Arg Arg Asn Ser Arg Gln Arg Tyr Lys Leu 
    770                 775                 780                 


Leu Asp Asp Gly Val Lys Asn Leu Ala Ser Asp Leu Asn Gly Asn Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asp Asn Gln Ala Leu Gln Asn Glu Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Ala 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Asn  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Ala Asp Lys  Leu Ile Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Ser Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Lys  Lys Gln Glu Phe Val  Asn Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Leu Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Ser Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1020
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1020

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Gln Glu Glu Lys Asp Tyr 
        115                 120                 125             


His Glu Lys Phe Ser Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Ile Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Ser Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Ser Lys Gln Tyr Gln Asp Phe Leu Glu Ile 
            180                 185                 190         


Phe Asn Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Gln Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Ile Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Asp Ser Glu Asn Phe Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Lys Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Ile Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Phe Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Lys Asn Glu Gln Gly Glu Thr Tyr Phe Phe Asp Ser Asn Ile 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Glu His Arg Lys Val Ser 
545                 550                 555                 560 


Lys Lys Lys Leu Leu Asp Phe Leu Ala Lys Glu Tyr Glu Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Val Ile Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Glu Lys Ile Leu Asp Lys Asp Phe 
        595                 600                 605             


Leu Asp Asn Pro Asp Asn Glu Ser Ile Leu Glu Asp Ile Val Gln Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Asn 
625                 630                 635                 640 


Tyr Lys Asp Leu Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Glu Ser Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Arg Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu 
    690                 695                 700                 


Ser Phe Lys Ser Ile Ile Ser Lys Ala Gln Ala Gly Ser His Ser Asp 
705                 710                 715                 720 


Asn Leu Lys Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly Tyr Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Asn Gln Gly Arg Arg Asn Ser Arg Gln Arg Tyr Lys Leu 
    770                 775                 780                 


Leu Asp Asp Gly Val Lys Asn Leu Ala Ser Asp Leu Asn Gly Asn Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asp Asn Gln Ala Leu Gln Asn Glu Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Ala 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Asn  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Asp Asp Lys  Leu Met Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Ser Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Lys  Lys Gln Glu Phe Val  Asn Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Leu Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Ser Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1021
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1021

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Tyr Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Arg Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Lys Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Arg Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Leu Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Arg Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Leu Leu Lys Glu Asn Leu Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Phe Leu Ala Glu Gly Phe Lys Asp Phe Gln Phe Leu Asn Arg Lys 
    530                 535                 540                 


Gln Lys Glu Thr Ile Phe Asn Ser Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Ser Phe Leu Asn Lys Val Asp Gly Tyr Glu 
                565                 570                 575     


Gly Ile Ala Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Gly Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Thr Asp Asn Glu Leu Ile Leu Glu Asp Ile Val Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Asp Ile Tyr Lys Asp 
625                 630                 635                 640 


Phe Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Ala Gly Leu Ser Phe Lys 
    690                 695                 700                 


Pro Ile Ile Asp Lys Ala Arg Thr Gly Ser His Ser Asp Asn Leu Lys 
705                 710                 715                 720 


Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Leu Ser Arg Ser Arg Gln Arg Leu Thr Thr Leu Arg Glu 
    770                 775                 780                 


Ser Leu Ala Asn Leu Lys Ser Asn Ile Leu Glu Glu Lys Lys Pro Lys 
785                 790                 795                 800 


Tyr Val Lys Asp Gln Val Glu Asn His His Leu Ser Asp Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Val Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asp  Ile Arg Asp Asp Lys  Leu Met Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Leu Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Gln  Lys Gln Glu Phe Val  Val Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Ile Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Asn Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Pro Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1022
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1022

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Phe Gly Asp Thr Asp Arg Ser His Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Thr Leu Leu Phe Asp Asp Gly Asn Thr Ala Glu Ser Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Thr Glu Ser Met Asn Glu Ile Asp Glu Ser 
                85                  90                  95      


Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Gln Glu Glu Lys Glu Tyr 
        115                 120                 125             


His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala Asp 
    130                 135                 140                 


Ser Lys Glu Lys Ser Asp Val Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Thr Phe Asp Ile 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Asn Ile Tyr 
            180                 185                 190         


Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             


Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 


Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     


Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Glu Glu Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             


Leu Phe Leu Ile Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     


Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Val Glu Val Phe Ser 
            340                 345                 350         


Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             


Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 


Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         


Leu Lys Glu Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Asp Asn Arg Asp Phe Ser Trp 
    450                 455                 460                 


Leu Thr Arg Asn Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Ala Arg Ser Ala Glu Asp Phe Ile His Arg Met Thr 
                485                 490                 495     


Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 


Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr 
545                 550                 555                 560 


Glu Lys Asp Ile Ile His Tyr Leu His Asn Val Asp Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Asn Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Lys Ile Thr Lys Asp Lys Glu Phe Met Asp Asp 
        595                 600                 605             


Pro Lys Asn Glu Glu Ile Leu Glu Asn Ile Val His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp Thr 
625                 630                 635                 640 


Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         


Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Lys Ile 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe Lys 
    690                 695                 700                 


Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asp Asp Val Lys 
705                 710                 715                 720 


Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Ala Leu Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp 
    770                 775                 780                 


Ala Leu Lys Asn Leu Ala Pro Gly Leu Asp Ser Asn Ile Leu Lys Glu 
785                 790                 795                 800 


Asn Pro Thr Asp Asn Ile Gln Leu Lys Asn Asp Arg Leu Phe Leu Tyr 
                805                 810                 815     


Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Lys Pro Leu Asp Ile 
            820                 825                 830         


Asn Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe 
        835                 840                 845             


Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Asp 
    850                 855                 860                 


Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val Val Gln Lys 
865                 870                 875                 880 


Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu 
                885                 890                 895     


Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu 
            900                 905                 910         


Arg Asp Lys Val Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Gln Gln 
        915                 920                 925             


Ile Thr Lys Asn Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu 
    930                 935                 940                 


Val Lys Glu Lys Asn Gln Lys Ile Arg Thr Val Lys Ile Ile Thr Leu 
945                 950                 955                 960 


Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly Leu Tyr Lys 
                965                 970                 975     


Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn 
            980                 985                 990         


Ala Val Val Ala Lys Ala Ile Leu  Lys Lys Tyr Pro Lys  Leu Glu Pro 
        995                 1000                 1005             


Glu Phe  Val Tyr Gly Asp Tyr  Gln Lys Tyr Asp Leu  Lys Lys Tyr 
    1010                 1015                 1020             


Ile Ser  Arg Phe Lys Pro Ser  Lys Glu Ile Glu Lys  Ala Thr Glu 
    1025                 1030                 1035             


Lys Tyr  Phe Phe Tyr Ser Asn  Leu Leu Asn Phe Phe  Lys Glu Glu 
    1040                 1045                 1050             


Val Leu  Tyr Ala Asp Gly Thr  Ile Arg Lys Arg Glu  Asn Ile Glu 
    1055                 1060                 1065             


Tyr Ser  Lys Asp Thr Gly Glu  Ile Ala Trp Asp Lys  Glu Lys Asp 
    1070                 1075                 1080             


Phe Ala  Thr Ile Lys Lys Val  Leu Ser Tyr Pro Gln  Val Asn Ile 
    1085                 1090                 1095             


Val Lys  Lys Arg Glu Val Gln  Thr Gly Gly Phe Ser  Lys Glu Ser 
    1100                 1105                 1110             


Ile Leu  Pro Lys Gly Asn Ser  Asp Lys Leu Ile Pro  Arg Lys Thr 
    1115                 1120                 1125             


Lys Asp  Ile Leu Trp Asp Thr  Thr Lys Tyr Gly Gly  Phe Asp Ser 
    1130                 1135                 1140             


Pro Val  Ile Ala Tyr Ser Ile  Leu Leu Ile Ala Asp  Ile Glu Lys 
    1145                 1150                 1155             


Gly Lys  Ala Lys Lys Leu Lys  Thr Val Lys Thr Leu  Val Gly Ile 
    1160                 1165                 1170             


Thr Ile  Met Glu Lys Ala Ala  Phe Glu Glu Asn Pro  Ile Thr Phe 
    1175                 1180                 1185             


Leu Glu  Asn Lys Gly Tyr His  Asn Val Arg Lys Glu  Asn Ile Leu 
    1190                 1195                 1200             


Cys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Arg 
    1205                 1210                 1215             


Arg Leu  Leu Ala Ser Ala Lys  Glu Leu Gln Lys Gly  Asn Glu Ile 
    1220                 1225                 1230             


Val Leu  Pro Val Cys Leu Thr  Thr Leu Leu Tyr His  Ser Lys Asn 
    1235                 1240                 1245             


Leu His  Lys Leu Asp Glu Pro  Glu His Leu Glu Tyr  Ile Gln Lys 
    1250                 1255                 1260             


His Arg  Asn Glu Phe Lys Asp  Leu Leu Asn Leu Val  Ser Glu Phe 
    1265                 1270                 1275             


Ser Gln  Lys Tyr Ile Leu Ala  Glu Ala Asn Leu Glu  Lys Ile Lys 
    1280                 1285                 1290             


Asp Leu  Tyr Ala Asp Asn Glu  Gln Ala Asp Ile Glu  Ile Leu Ala 
    1295                 1300                 1305             


Asn Ser  Phe Ile Asn Leu Leu  Thr Phe Thr Ala Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Gly  Lys Asp Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Thr  Val Ser Glu Ile Leu  Asn Ala Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Lys Leu Gly 
    1355                 1360                 1365             


Glu Glu  
    1370 


<210>  1023
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1023

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Asn Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Leu Leu 
65                  70                  75                  80  


Tyr Leu Gln Asp Ile Phe Ser Asp Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Ser Glu Asp Lys Arg 
            100                 105                 110         


Gly Asp His His Pro Ile Phe Gly Asn Val Val Asp Glu Val Lys Tyr 
        115                 120                 125             


His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 


Thr Pro Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Asp Asp Pro Lys Leu Asp 
                165                 170                 175     


Val Lys Asn Thr Asp Ser Gln Glu Leu Phe Ser Gln Phe Leu Thr Ala 
            180                 185                 190         


Tyr Asp Val Thr Gln Glu Asp Ser His Leu Ala Asp Val Lys Ile Asn 
        195                 200                 205             


Ile Ser Asp Ile Ile Thr Glu Lys Met Asn Lys Ser Arg Lys Val Glu 
    210                 215                 220                 


Ala Leu Leu Phe His Phe Pro Ser Gln Lys Lys Asn Ser Phe Phe Gly 
225                 230                 235                 240 


Asn Leu Val Ser Leu Val Phe Gly Leu Leu Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Asn Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Thr 
        275                 280                 285             


Asp Val Phe Val Ala Ala Arg Lys Leu Tyr Asp Ser Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Asn Asp Leu Ser Thr Lys Ala Ile Leu Ser Ala 
305                 310                 315                 320 


Ser Met Val Arg Arg Tyr Glu Glu His Asn Lys Asp Leu Ala Lys Leu 
                325                 330                 335     


Lys Ala Phe Val Lys Ala Asn Phe Thr His Asp Val Phe Lys Glu Val 
            340                 345                 350         


Phe Ser Asp Ala Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys 
        355                 360                 365             


Thr Ser Gln Glu Asp Phe Tyr Lys Tyr Val Lys Asn Leu Ile Gly Lys 
    370                 375                 380                 


Val Asp Gly Ser Ala Asp Phe Ile Glu Lys Ile Asp Arg Glu Asp Phe 
385                 390                 395                 400 


Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val 
                405                 410                 415     


His Leu Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr 
            420                 425                 430         


Pro Phe Leu Lys Glu His Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe 
        435                 440                 445             


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asp Arg His Gly His Ser Asp 
    450                 455                 460                 


Phe Ala Trp Ala Ser Phe Asn Ser Glu Glu Lys Val Thr Pro Trp Asn 
465                 470                 475                 480 


Phe Glu Glu Ile Ile Asp Lys Asn Lys Ser Ala Gln Ala Phe Ile Glu 
                485                 490                 495     


Arg Met Thr Asn Gln Asp Leu Tyr Leu Pro Asn Glu Lys Val Leu Pro 
            500                 505                 510         


Lys His Ser Pro Leu Tyr Glu Lys Tyr Thr Val Tyr Asn Glu Leu Thr 
        515                 520                 525             


Lys Ile Lys Tyr Val Thr Glu Thr Gly Thr Ala Lys Phe Phe Asp Ala 
    530                 535                 540                 


Asn Leu Lys Gln Glu Ile Phe Asp Gly Leu Phe Lys Lys Glu Arg Lys 
545                 550                 555                 560 


Val Thr Lys Arg Lys Leu Leu Thr Phe Leu Glu Lys Asn Phe Asp Glu 
                565                 570                 575     


Phe Arg Ile Val Asp Ile Gln Gly Leu Asp Lys Glu Thr Glu Thr Phe 
            580                 585                 590         


Asn Ala Ser Tyr Gly Ile Tyr Gln Asp Leu Leu Lys Ile Ile Lys Asp 
        595                 600                 605             


Lys Ala Phe Met Asp Asp Ser Glu Asn Ala Asp Ile Leu Glu Asp Ile 
    610                 615                 620                 


Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Lys Gln Arg 
625                 630                 635                 640 


Leu Ala Lys Tyr Ala Asp Leu Phe Asp Lys Lys Val Ile Asp Asn Leu 
                645                 650                 655     


Ala Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu 
            660                 665                 670         


Asn Gly Ile Arg Asp Lys Arg Ser Arg Lys Thr Ile Met Asp Tyr Leu 
        675                 680                 685             


Val Asp Asp Gly Arg Ser Asn Arg Asn Leu Met Gln Leu Ile Thr Asp 
    690                 695                 700                 


Asp Asn Leu Thr Phe Lys Asp Glu Ile Ala Lys Ala Gln Tyr Ser Asp 
705                 710                 715                 720 


Asn Ser Asp Asp Leu His Gln Val Val Gln Asp Leu Ala Gly Ser Pro 
                725                 730                 735     


Ala Ile Lys Lys Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu 
            740                 745                 750         


Val Lys Val Met Gly Tyr Glu Pro Glu Gln Ile Val Val Glu Met Ala 
        755                 760                 765             


Arg Glu Asn Gln Thr Thr Ala Lys Gly Arg Arg Asn Ser Gln Gln Arg 
    770                 775                 780                 


Tyr Lys Arg Leu Glu Glu Ala Ile Lys Asn Leu Asp Arg Asp Leu Asn 
785                 790                 795                 800 


Tyr Lys Ile Leu Lys Glu His Pro Thr Asp Asn Gln Ala Leu Gln Asn 
                805                 810                 815     


Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr 
            820                 825                 830         


Gly Glu Ser Leu Asp Ile Asn Arg Leu Ser Asp Tyr Asp Ile Asp His 
        835                 840                 845             


Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn Ser Ile Asp Asn Arg Val 
    850                 855                 860                 


Leu Val Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser 
865                 870                 875                 880 


Glu Asp Val Val Asn Arg Met Arg Pro Phe Trp Asn Lys Leu Leu Asn 
                885                 890                 895     


Cys Gly Leu Ile Ser Gln Arg Lys Tyr Ser Asn Leu Thr Lys Lys Glu 
            900                 905                 910         


Leu Thr Pro Asn Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu His Phe 
    930                 935                 940                 


Asn Lys Glu Phe Asp Glu Asp Lys Lys Arg Ile Arg Lys Val Lys Val 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu 
                965                 970                 975     


Leu Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Leu Leu Met Lys  Tyr Pro Lys 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Glu Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Arg  Glu Arg Lys Thr Ala  Thr Gln Arg Met Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Met Phe Lys  Ser Lys Val Lys Leu  Ala Asp Glu 
    1040                 1045                 1050             


Arg Ile  Ile Glu Arg Pro Met  Ile Glu Val Asn Asp  Glu Thr Gly 
    1055                 1060                 1065             


Glu Ile  Ala Trp Asp Lys Thr  Lys His Ile Ala Thr  Val Lys Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Ile  Asn Ile Val Lys Lys  Val Glu Glu 
    1085                 1090                 1095             


Gln Thr  Gly Gly Leu Thr Asn  Glu Thr Ile Tyr Pro  Arg Gly Ser 
    1100                 1105                 1110             


Phe Asp  Lys Leu Leu Asn Arg  Lys Asn Thr Leu Glu  Ala Ser Lys 
    1115                 1120                 1125             


Tyr Gly  Gly Phe Gly Ser Pro  Thr Val Ala Tyr Cys  Val Leu Val 
    1130                 1135                 1140             


Val Ala  Asp Ser Lys Asn Glu  Asn Gly Lys Val Lys  Lys Leu Lys 
    1145                 1150                 1155             


Leu Gly  Ser Glu Leu Leu Gly  Ile Thr Ile Leu Glu  Lys Asn Asp 
    1160                 1165                 1170             


Phe Glu  Asn Asn Ser Leu Asp  Phe Leu Ser Arg Lys  Gly Tyr Gln 
    1175                 1180                 1185             


Gly Val  Gln Glu Lys Lys Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Phe Gln Asp Gly Ser  Arg Arg Met Leu Ser  Ser Val Met 
    1205                 1210                 1215             


Ala Asn  Asp Asn Lys Arg Gly  Asp Leu Gln Lys Ala  Asn Glu Met 
    1220                 1225                 1230             


Ile Leu  Pro Ala Tyr Leu Val  Lys Leu Leu Tyr His  Ala Lys His 
    1235                 1240                 1245             


Ile Asn  Ser Val Gly Glu Pro  Ala His Lys Asn Tyr  Ile Asn Glu 
    1250                 1255                 1260             


His Cys  Ser Glu Phe Gln Asp  Leu Leu Asn Tyr Ile  Arg Gln Phe 
    1265                 1270                 1275             


Ala Asp  Lys Phe Val Asp Val  Asp Lys Asn Leu Ser  Lys Ile Arg 
    1280                 1285                 1290             


Ser Leu  Ser Lys Ser Met Ala  Glu Tyr Ser Val Ser  Glu Ile Cys 
    1295                 1300                 1305             


Ser Ser  Phe Val Asn Leu Leu  Thr Leu Thr Ala Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Asp  Phe Lys Phe Phe Gly  Thr Thr Ile Pro Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Gly Ser  Pro Gln Ser Ile Leu  Ser Ala Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1024
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1024

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Tyr Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Arg Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Lys Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Arg Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Leu Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Arg Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Leu Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Phe Leu Ala Glu Gly Phe Lys Asp Phe Gln Phe Leu Asn Arg Lys 
    530                 535                 540                 


Gln Lys Glu Thr Ile Phe Asn Ser Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Ser Phe Leu Asn Lys Val Asp Gly Tyr Glu 
                565                 570                 575     


Gly Ile Ala Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Gly Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Thr Asp Asn Glu Leu Ile Leu Glu Asp Ile Val Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Asp Ile Tyr Lys Asp 
625                 630                 635                 640 


Phe Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Ala Gly Leu Ser Phe Lys 
    690                 695                 700                 


Pro Ile Ile Asp Lys Ala Arg Thr Gly Ser His Ser Asp Asn Leu Lys 
705                 710                 715                 720 


Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Leu Ser Arg Leu Arg Gln Arg Leu Thr Thr Leu Arg Glu 
    770                 775                 780                 


Ser Leu Ala Asn Leu Lys Ser Asn Ile Leu Glu Glu Lys Lys Pro Lys 
785                 790                 795                 800 


Tyr Val Lys Asp Gln Val Glu Asn His His Leu Ser Asp Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Val Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Asp Asp Lys  Leu Met Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Asp Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Leu Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Gln  Lys Gln Glu Phe Val  Val Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Ile Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Asn Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Pro Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1025
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1025

Met Glu Lys Asn Lys Glu Tyr Val Ile Gly Leu Asp Ile Gly Thr Gly 
1               5                   10                  15      


Ser Val Gly Trp Ser Ala Ile Ser Thr Asp Asn Tyr Gln Leu Ile Arg 
            20                  25                  30          


Gln Lys Gly Lys Asn Leu Ile Gly Val Arg Leu Phe Asn Ser Ala Glu 
        35                  40                  45              


Thr Ala Glu Glu Arg Arg Gly Tyr Arg Thr Thr Arg Arg Arg Leu Ser 
    50                  55                  60                  


Arg Arg Arg Trp Arg Met Ser Leu Leu Asn Asp Ile Phe Arg Pro Glu 
65                  70                  75                  80  


Leu Asn Glu Ile Asp Glu Asn Phe Leu Ala Arg Leu Lys Tyr Ser Trp 
                85                  90                  95      


Val Ser Pro Leu Asp Gln Lys Asn Pro Gln Leu Asn Glu Gln Gly Thr 
            100                 105                 110         


Asn Ala Ser Leu Tyr Gly Asp Ile Glu Ala Asp Gln Ala Phe His Arg 
        115                 120                 125             


Ala Tyr Pro Thr Ile Tyr His Leu Arg Lys Thr Leu Met Glu Asp Asn 
    130                 135                 140                 


Gln Lys His Asp Leu Arg Glu Val Tyr Leu Ala Ile His His Ile Val 
145                 150                 155                 160 


Lys Tyr Arg Gly His Phe Leu Thr Pro Asp Phe Asp Ala Asn Ser Gln 
                165                 170                 175     


Thr Ile Asp Ile Lys Gly Leu Phe Lys Gln Leu Gly Asp Val Ser Gly 
            180                 185                 190         


Ser Ile Glu Leu Gln Ala Asp Gln Thr Ser Thr Val Ile Ala Ala Phe 
        195                 200                 205             


Gln Ser Ile Leu Leu Asp Phe Thr Lys Thr Pro Thr Glu Ser Gly Arg 
    210                 215                 220                 


Leu Ala Gly Gln Leu Phe Ser Asp Gln Asn Lys Asp Lys Gln Ala Lys 
225                 230                 235                 240 


Lys Gln Val Glu Glu Leu Phe Lys Leu Leu Arg Gly Gly Lys Gly Ser 
                245                 250                 255     


Leu Ala Asp Met Phe Leu Leu Thr Asp Leu Asp Lys Val Val Lys Asn 
            260                 265                 270         


Gln Leu Lys Phe Ala Phe Asp Asp Ala Val Asp Glu Asn Leu Asn Leu 
        275                 280                 285             


Ile Ser Asn Leu Ile Asn Glu Glu Gln Tyr Glu Leu Leu Leu Asn Ile 
    290                 295                 300                 


Gln Lys Leu Arg Gly Thr Ile Ile Leu Lys Gln Ile Leu Gly Asp Asp 
305                 310                 315                 320 


Glu Thr Val Ser Asp Ala Lys Val Arg Ser Tyr Glu Glu His Ala His 
                325                 330                 335     


Gln Leu Lys Glu Leu Lys Ala Gln Val Leu Thr Lys Asp Asn Lys Glu 
            340                 345                 350         


Lys Phe Glu Glu Ala Tyr Gln Leu Ile Gln Lys Gly Asp Ala Ser Arg 
        355                 360                 365             


Ile Asp Gly Leu Lys Lys Leu Lys Thr Ile Ile Asn Gln Glu His Glu 
    370                 375                 380                 


Ala Leu Arg Leu Asp Lys Tyr Glu Ala Asp Lys Leu Leu Lys Leu Ile 
385                 390                 395                 400 


Glu Leu Asp Gln Leu Leu Pro Val Gln Arg Ser Lys Arg Asn Gly Glu 
                405                 410                 415     


Ile Pro His Gln Leu His Glu Phe Glu Leu Lys Lys Ile Ile Glu Lys 
            420                 425                 430         


Gln Gly Arg Tyr Tyr Pro Phe Leu Arg Glu Thr Phe Glu Arg Asp Gly 
        435                 440                 445             


Gln Glu Phe Asn Lys Ile Gly Gln Leu Val Asn Phe Arg Ile Pro Tyr 
    450                 455                 460                 


Tyr Val Gly Pro Leu Val Asp Pro Arg Leu Ala Gly Ile Gln Ser Gly 
465                 470                 475                 480 


Asp Gln Thr Asn His Trp Met Thr Gln Lys Lys Ala Gly Lys Ile Thr 
                485                 490                 495     


Pro Trp Asn Tyr Thr Glu Leu Ile Asp Ile Asp Lys Ser Ala Ala Glu 
            500                 505                 510         


Phe Ile Lys Arg Met Thr Gly Thr Asp Thr Tyr Leu Val Gly Glu Pro 
        515                 520                 525             


Ser Leu His Lys Ala Ser Leu Ile Tyr Gln Asp Tyr Asn Val Leu Gln 
    530                 535                 540                 


Glu Leu Asn Asn Leu Arg Tyr Asp Gly Lys Pro Leu Pro Val Asp Ala 
545                 550                 555                 560 


Lys Gln Glu Ile Tyr Ser Asn Leu Gln Val Ser Leu Lys Asn Ala Thr 
                565                 570                 575     


Lys Asn Lys Ile Asn Ser Phe Leu Ile Ser Glu Gly Tyr Leu Gln Asp 
            580                 585                 590         


Gly Glu Glu Leu Thr Gly Phe Ala Gly Lys Glu Leu Lys Asn Lys Leu 
        595                 600                 605             


Ser Ser Phe His Tyr Phe Lys Asn Ile Phe Gly Pro Glu Arg Met Asn 
    610                 615                 620                 


Thr Leu Gly Thr Asp Asn Leu Glu Lys Ile Thr Glu Leu Gln Thr Val 
625                 630                 635                 640 


Phe Glu Asp Arg His Met Leu Lys Arg Gln Leu Gln Lys Leu Gly Phe 
                645                 650                 655     


Leu Asp Asp Asp Glu Cys Glu Lys Leu Ala Gly Lys His Phe Thr Gly 
            660                 665                 670         


Trp Gly Asn Leu Ser Lys Lys Leu Leu Asn Thr Pro Ala Ile Ile Asp 
        675                 680                 685             


Pro Glu Ile Asn Glu Asn Lys Ile Ser Leu Ile Asp Ala Leu Tyr Lys 
    690                 695                 700                 


Gly Gln Arg Asn Leu Ser Phe Tyr Val Asn Asn Met Asp Asp Lys Tyr 
705                 710                 715                 720 


Gln Phe Arg Asp Trp Ile Glu Ser Glu Asn Gln Ser Gln Gln Val Glu 
                725                 730                 735     


Ile Ser Leu Asp Glu Lys Ile Lys Asn Leu Ala Gly Ser Pro Ala Ile 
            740                 745                 750         


Lys Arg Gly Ile Thr Gln Ser Met Ala Val Ile Gln Asp Ile Ile Arg 
        755                 760                 765             


Ala Asn Asn Gly Ile Ser Pro Lys Arg Ile Tyr Leu Glu Phe Ala Arg 
    770                 775                 780                 


Glu Ser Gln Asn Ser Tyr Gln Pro Asn Ser Arg Ile Lys Gln Ile Glu 
785                 790                 795                 800 


Lys Ile Tyr Asp Gln Asn Lys Lys Glu Leu Met Lys Val Tyr Pro Asp 
                805                 810                 815     


Val Phe Lys Asp Val Gln Gly Val Leu Asn Asn Val Asp Glu Asn Asn 
            820                 825                 830         


Ser Leu Ser Glu Lys Ala Phe Leu Tyr Phe Met Gln Leu Gly Lys Asp 
        835                 840                 845             


Met Tyr Thr Gly Glu Ala Leu Asp Phe Asp Lys Leu Tyr Thr Tyr Asp 
    850                 855                 860                 


Ile Asp His Ile Ile Pro Gln Ala Met Thr Lys Asp Asn Ser Trp Asp 
865                 870                 875                 880 


Asn Lys Val Leu Val Thr Ala Ile Lys Asn Arg Glu Lys Ser Asn Gln 
                885                 890                 895     


Val Leu Asp Asn Ala Ile Gln Ala Lys Met His Asn Phe Trp Leu Gln 
            900                 905                 910         


Leu Lys Lys Val Asn Leu Leu Ser Gln Arg Lys Tyr Asp Thr Leu Met 
        915                 920                 925             


Lys Thr Asn Phe Ser Lys Gly Asp Glu Lys Lys Phe Ile Ala Arg Ser 
    930                 935                 940                 


Leu Val Glu Thr Arg Gln Ile Ile Lys Asn Val Ala Glu Leu Ile Lys 
945                 950                 955                 960 


Ser Glu Phe Asp His Val Glu Ala Ile Pro Ile Arg Gln Glu Leu Thr 
                965                 970                 975     


Ser Asp Met Arg Gln Leu Met Asp Tyr Lys Lys Asn Arg Leu Val Asn 
            980                 985                 990         


Asp Tyr His His Ala His Asp Ala  Leu Leu Ile Ala Thr  Val Gly Glu 
        995                 1000                 1005             


Phe Met  Gln Lys Arg Gly Phe  Met Gln Gly Met Asn  Pro Ser Gln 
    1010                 1015                 1020             


Asn Val  Tyr His Gly Tyr Thr  Gln Tyr Val Lys Lys  Trp Leu Ser 
    1025                 1030                 1035             


Met Ala  Lys Lys Ala Arg Ser  Ala Glu Lys Pro Glu  Arg Ile Phe 
    1040                 1045                 1050             


Pro Leu  Gln Phe Val Val Gly  Ser Met Arg Met Ala  Val Arg Asp 
    1055                 1060                 1065             


Gly Gln  Val Asp Lys Val Thr  His Pro Ala Thr Gly  Glu Ile Cys 
    1070                 1075                 1080             


Trp Thr  Ala Asp Asn Glu Ala  Tyr Leu Arg Lys Val  Leu Gly Tyr 
    1085                 1090                 1095             


Lys Asn  Ile Leu Val Thr Arg  Lys Val Lys Asn Ala  Gly Lys Leu 
    1100                 1105                 1110             


Tyr Gln  Arg Val Thr Val Lys  Pro Lys Gly Ser Gly  Glu Ala Pro 
    1115                 1120                 1125             


Val Asn  His Gln Lys Ser Asp  Ile Asn Ile Tyr Gly  Gly Val Thr 
    1130                 1135                 1140             


Ser Glu  Gln Ala Ala Glu Leu  Val Leu Ile Arg Ile  Lys Ala Lys 
    1145                 1150                 1155             


Asn Asn  Ala Lys Asn Met Val  Ile Lys Ile Pro Thr  Ala Ile Ala 
    1160                 1165                 1170             


Asn Gln  Val Ala Asn Lys Thr  Thr Thr Tyr Asp Ala  Tyr Val Arg 
    1175                 1180                 1185             


Ser Leu  Asn Ile Pro Asn Tyr  Ala Gly Ile Ile Leu  Asp Gln Ile 
    1190                 1195                 1200             


Pro Leu  Gly Gln Leu Val Phe  Asp Glu Arg Thr Ser  Phe Tyr Leu 
    1205                 1210                 1215             


Ala Ser  Ala Glu Ile Lys His  Asn Ala Arg Gln Leu  Trp Val Asn 
    1220                 1225                 1230             


Gln His  Ile Asn Asn Val Leu  Thr Lys Lys Asn Ile  Asn Asp Glu 
    1235                 1240                 1245             


Glu Arg  Asp Asn Ile Leu Asn  Leu Leu Thr Asn Glu  Lys Asn Leu 
    1250                 1255                 1260             


Glu Arg  Phe Lys Leu Leu Glu  Pro Asp Leu Lys Arg  Val Lys Ala 
    1265                 1270                 1275             


Ala Lys  Gln Gln Trp Ala Ser  Cys Asp Asp Gln Glu  Gln Met Gln 
    1280                 1285                 1290             


Ile Leu  Arg Ile Ile Ile Asn  Lys Met His Asp Asp  Ala Ser Pro 
    1295                 1300                 1305             


Gln Ile  Pro Gly Lys Ile Asp  Pro Phe Lys Asn Tyr  Leu Asp Lys 
    1310                 1315                 1320             


Gln Gly  Lys Gly Trp Arg Asp  Leu Arg Thr Lys Lys  Gly Met Lys 
    1325                 1330                 1335             


Leu Ser  Asp Asn Ala Glu Phe  Val Tyr Gln Ser Pro  Thr Gly Leu 
    1340                 1345                 1350             


Tyr His  Arg Lys Ile Ser Ile  Asn Glu Leu Leu Lys  Gln Lys Gln 
    1355                 1360                 1365             


Leu Glu  
    1370 


<210>  1026
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1026

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Tyr Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Arg Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Lys Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Arg Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Leu Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Arg Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Leu Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Phe Leu Ala Glu Gly Phe Lys Asp Phe Gln Phe Leu Asn Arg Lys 
    530                 535                 540                 


Gln Lys Glu Thr Ile Phe Asn Ser Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Ser Phe Leu Asn Lys Val Asp Gly Tyr Glu 
                565                 570                 575     


Gly Ile Ala Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Gly Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Thr Asp Asn Glu Leu Ile Leu Glu Asp Ile Val Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Asp Ile Tyr Lys Asp 
625                 630                 635                 640 


Phe Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Ala Gly Leu Ser Phe Lys 
    690                 695                 700                 


Pro Ile Ile Asp Lys Ala Arg Thr Gly Ser His Leu Asp Asn Leu Lys 
705                 710                 715                 720 


Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Leu Ser Arg Ser Arg Gln Arg Leu Thr Thr Leu Arg Glu 
    770                 775                 780                 


Ser Leu Ala Asn Leu Lys Ser Asn Ile Leu Glu Glu Lys Lys Pro Lys 
785                 790                 795                 800 


Tyr Val Lys Asp Gln Val Glu Asn His His Leu Ser Asp Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Val 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Thr Asp Lys  Leu Ile Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Tyr 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Leu Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Gln  Lys Gln Glu Phe Val  Val Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Ile Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Asn Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Pro Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Gly  
    1370 


<210>  1027
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1027

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Gln Glu Glu Lys Asp Tyr 
        115                 120                 125             


His Glu Lys Phe Ser Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Ile Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Ser Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Ser Lys Gln Tyr Gln Asp Phe Leu Glu Ile 
            180                 185                 190         


Phe Asn Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Gln Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Ile Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Asp Ser Glu Asn Phe Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Lys Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Ile Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Phe Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Lys Asn Glu Gln Gly Glu Thr Tyr Phe Phe Asp Ser Asn Ile 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Glu His Arg Lys Val Ser 
545                 550                 555                 560 


Lys Lys Lys Leu Leu Asp Phe Leu Ala Lys Glu Tyr Glu Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Val Ile Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Glu Lys Ile Leu Asp Lys Asp Phe 
        595                 600                 605             


Leu Asp Asn Pro Asp Asn Glu Ser Ile Leu Glu Asp Ile Val Gln Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Asn 
625                 630                 635                 640 


Tyr Lys Asp Leu Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Glu Ser Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Arg Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu 
    690                 695                 700                 


Ser Phe Lys Ser Ile Ile Ser Lys Ala Gln Ala Gly Ser His Ser Asp 
705                 710                 715                 720 


Asn Leu Lys Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly Tyr Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Asn Gln Gly Arg Arg Asn Ser Arg Gln Arg Tyr Lys Leu 
    770                 775                 780                 


Leu Asp Asp Gly Val Lys Asn Leu Ala Ser Asp Leu Asn Gly Asn Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asp Asn Gln Ala Leu Gln Asn Glu Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Ala 
            820                 825                 830         


Leu Asp Ile Asp Asp Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Asn  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Ala Asp Lys  Leu Ile Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Ser Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Lys  Lys Gln Glu Phe Val  Asn Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Leu Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Ser Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1028
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1028

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Gln Glu Glu Lys Asp Tyr 
        115                 120                 125             


His Glu Lys Phe Ser Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Arg Ala Asp Leu Arg Leu Ile Tyr Ile Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Ser Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Ser Lys Gln Tyr Gln Asp Phe Leu Glu Ile 
            180                 185                 190         


Phe Asn Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Gln Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Ile Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Asp Ser Glu Asn Phe Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Lys Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Ile Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Phe Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Lys Asn Glu Gln Gly Glu Thr Tyr Phe Phe Asp Ser Asn Ile 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Glu His Arg Lys Val Ser 
545                 550                 555                 560 


Lys Lys Lys Leu Leu Asp Phe Leu Ala Lys Glu Tyr Glu Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Val Ile Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Glu Lys Ile Leu Asp Lys Asp Phe 
        595                 600                 605             


Leu Asp Asn Pro Asp Asn Glu Ser Ile Leu Glu Asp Ile Val Gln Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Asn 
625                 630                 635                 640 


Tyr Lys Asp Leu Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Glu Ser Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Arg Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu 
    690                 695                 700                 


Ser Phe Lys Ser Ile Ile Ser Lys Ala Gln Ala Gly Ser His Ser Asp 
705                 710                 715                 720 


Asn Leu Lys Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly Tyr Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Asn Gln Gly Arg Arg Asn Ser Arg Gln Arg Tyr Lys Leu 
    770                 775                 780                 


Leu Asp Asp Gly Val Lys Asn Leu Ala Ser Asp Leu Asn Gly Asn Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asp Asn Gln Ala Leu Gln Asn Glu Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Ala 
            820                 825                 830         


Leu Asp Ile Asp Asp Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Asn  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Ala Asp Lys  Leu Ile Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Ser Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Lys  Lys Gln Glu Phe Val  Asn Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Leu Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Ser Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1029
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1029

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ser Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Asp Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Asp Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Phe Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Ser Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Arg Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn His Leu Leu Ser Gln Asn Ile Asp 
        195                 200                 205             


Val Glu Gly Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Gln Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Val Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Thr Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Gly Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Phe Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Lys Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Leu Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Leu Val Asp Lys Glu Ala Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Lys Asn Glu Gln Gly Glu Thr Tyr Phe Phe Asp Ser Asn Ile 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Glu His Arg Lys Val Ser 
545                 550                 555                 560 


Lys Lys Lys Leu Leu Asp Phe Leu Ala Lys Glu Tyr Glu Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Val Ile Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys Asp Phe 
        595                 600                 605             


Leu Asp Asn Pro Asp Asn Glu Ser Ile Leu Glu Asp Ile Val Gln Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Asn 
625                 630                 635                 640 


Tyr Lys Asp Leu Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Glu Ser Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Lys Ser Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Gly Leu 
    690                 695                 700                 


Ser Phe Lys Ser Ile Ile Ser Lys Ala Gln Ala Gly Ser His Ser Asp 
705                 710                 715                 720 


Asn Leu Lys Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly Tyr Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Asn Gln Gly Arg Arg Asn Ser Arg Gln Arg Tyr Lys Leu 
    770                 775                 780                 


Leu Asp Asp Gly Val Lys Asn Leu Ala Ser Asp Leu Asn Gly Asn Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asp Asn Gln Ala Leu Gln Asn Glu Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Lys Ala 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Asp Asp Lys  Leu Met Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Leu Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Gln  Lys Gln Glu Phe Val  Val Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Ile Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Asn Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Ser Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1030
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1030

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Tyr Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Arg Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Lys Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Arg Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln His Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Leu Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Arg Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Leu Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Phe Leu Ala Glu Gly Phe Lys Asp Phe Gln Phe Leu Asn Arg Lys 
    530                 535                 540                 


Gln Lys Glu Thr Ile Phe Asn Ser Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Ser Phe Leu Asn Lys Val Asp Gly Tyr Glu 
                565                 570                 575     


Gly Ile Ala Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Gly Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Thr Asp Asn Glu Leu Ile Leu Glu Asp Ile Val Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Asp Ile Tyr Lys Asp 
625                 630                 635                 640 


Phe Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Ala Gly Leu Ser Phe Lys 
    690                 695                 700                 


Pro Ile Ile Asp Lys Ala Arg Thr Gly Ser His Ser Asp Asn Leu Lys 
705                 710                 715                 720 


Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Leu Ser Arg Ser Arg Gln Arg Leu Thr Thr Leu Arg Glu 
    770                 775                 780                 


Ser Leu Ala Asn Leu Lys Ser Asn Ile Leu Glu Glu Lys Lys Pro Lys 
785                 790                 795                 800 


Tyr Val Lys Asp Gln Val Glu Asn His His Leu Ser Asp Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Val Glu Ile 
865                 870                 875                 880 


Val Lys Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Pro Lys  Tyr Asn Ser 
    1010                 1015                 1020             


Tyr Lys  Thr Arg Lys Ser Ala  Thr Glu Lys Leu Phe  Phe Tyr Ser 
    1025                 1030                 1035             


Asn Ile  Met Asn Phe Phe Lys  Thr Lys Val Thr Leu  Ala Asp Gly 
    1040                 1045                 1050             


Thr Val  Val Val Lys Asp Asp  Ile Glu Val Asn Asn  Asp Thr Gly 
    1055                 1060                 1065             


Glu Ile  Val Trp Asp Lys Lys  Lys His Phe Ala Thr  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Ile 
    1085                 1090                 1095             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Ala  His Gly Asn 
    1100                 1105                 1110             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Asp Ile  Tyr Leu Asp 
    1115                 1120                 1125             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Ile Val  Ala Tyr Ser 
    1130                 1135                 1140             


Val Leu  Val Val Ala Asp Ile  Lys Lys Gly Lys Ala  Gln Lys Leu 
    1145                 1150                 1155             


Lys Thr  Val Thr Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1160                 1165                 1170             


Arg Phe  Glu Lys Asn Pro Ser  Ala Phe Leu Glu Ser  Lys Gly Tyr 
    1175                 1180                 1185             


Leu Asn  Ile Arg Asp Asp Lys  Leu Met Ile Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg Leu Leu  Ala Ser Ala 
    1205                 1210                 1215             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Thr Gln Phe 
    1220                 1225                 1230             


Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr Asn Glu  Leu Lys Gly 
    1235                 1240                 1245             


Lys Pro  Glu Glu Ile Glu Gln  Lys Gln Glu Phe Val  Val Gln His 
    1250                 1255                 1260             


Val Ser  Tyr Phe Asp Asp Ile  Leu Gln Ile Ile Asn  Asp Phe Ser 
    1265                 1270                 1275             


Asn Arg  Val Ile Leu Ala Asp  Ala Asn Leu Glu Lys  Ile Asn Lys 
    1280                 1285                 1290             


Leu Tyr  Gln Asp Asn Lys Glu  Asn Ile Pro Val Asp  Glu Leu Ala 
    1295                 1300                 1305             


Asn Asn  Ile Ile Asn Leu Phe  Thr Phe Thr Ser Leu  Gly Ala Pro 
    1310                 1315                 1320             


Ala Ala  Phe Lys Phe Phe Asp  Lys Ile Val Asp Arg  Lys Arg Tyr 
    1325                 1330                 1335             


Thr Ser  Thr Lys Glu Val Leu  Asn Ser Thr Leu Ile  His Gln Ser 
    1340                 1345                 1350             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1355                 1360                 1365             


Glu Asp  
    1370 


<210>  1031
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1031

Met Lys Val Asn Asn Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Trp Val Ala Ile Gly Glu Asp Gly Lys Pro Leu Arg Ile Lys 
            20                  25                  30          


Gly Lys Thr Ala Ile Gly Ala Arg Leu Phe Gln Glu Gly Asn Pro Ala 
        35                  40                  45              


Ala Asp Arg Arg Met Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Lys Leu Leu Glu Glu Ile Phe Asp Pro Tyr Ile Thr 
65                  70                  75                  80  


Pro Val Asp Ser Thr Phe Phe Ala Arg Leu Lys Gln Ser Asn Leu Ser 
                85                  90                  95      


Pro Lys Asp Ser Arg Lys Glu Phe Lys Gly Ser Met Leu Phe Pro Asp 
            100                 105                 110         


Leu Thr Asp Met Gln Tyr His Lys Asp Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg His Ala Leu Met Thr Gln Asp Glu Lys Phe Asp Ile Arg Met Val 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn 
145                 150                 155                 160 


Ser Thr Pro Val Asp Ser Phe Lys Ala Ser Lys Val Asn Phe Val Asp 
                165                 170                 175     


Gln Phe Lys Lys Leu Asn Glu Leu Tyr Thr Ala Ile Asn Pro Glu Glu 
            180                 185                 190         


Ser Phe Gln Ile Asn Leu Ala Asn Ser Glu Asp Ile Gly His Gln Phe 
        195                 200                 205             


Leu Asp Pro Ser Ile Arg Lys Phe Asp Lys Lys Lys Gln Ile Pro Lys 
    210                 215                 220                 


Ile Val Pro Val Ser Val Asp Asp Lys Ala Thr Asp Lys Ile Asn Gly 
225                 230                 235                 240 


Lys Ile Ala Ser Glu Ile Ile Asn Ala Ile Leu Gly Tyr Lys Ser Lys 
                245                 250                 255     


Leu Asp Val Val Val Gln Cys Thr Pro Val Asp Ser Lys Ser Trp Ala 
            260                 265                 270         


Leu Lys Phe Asp Glu Glu Asp Ile Asp Ala Lys Leu Gln Lys Ile Leu 
        275                 280                 285             


Pro Glu Met Asp Glu Asn Gln Gln Ser Ile Ile Ala Ile Leu Gln Asn 
    290                 295                 300                 


Leu Tyr Ser Gln Val Thr Leu Asn Gln Ile Val Pro Asn Gly Met Ser 
305                 310                 315                 320 


Leu Ser Glu Ser Met Ile Glu Lys Tyr Asn Asp His His Asp His Leu 
                325                 330                 335     


Lys Leu Tyr Lys Lys Ile Ile Asp Gln Leu Ala Asp Pro Lys Lys Lys 
            340                 345                 350         


Ala Ala Leu Lys Lys Ala Tyr Ser Gln Tyr Val Gly Asp Asp Gly Lys 
        355                 360                 365             


Val Ile Glu Gln Ala Asp Phe Trp Ser Ser Val Lys Lys Asn Leu Asp 
    370                 375                 380                 


Asp Ser Asp Leu Ser Lys Gln Ile Met Asp Leu Ile Asp Ala Glu Lys 
385                 390                 395                 400 


Phe Met Pro Lys Gln Arg Thr Ser Gln Asn Gly Val Ile Pro His Gln 
                405                 410                 415     


Leu His Gln Arg Glu Leu Asp Glu Ile Ile Glu His Gln Ser Lys Tyr 
            420                 425                 430         


Tyr Pro Trp Leu Ala Glu Ile Asn Pro Asn Lys His Asp Leu His Leu 
        435                 440                 445             


Ala Lys Tyr Lys Ile Glu Glu Leu Val Ala Phe Arg Val Pro Tyr Tyr 
    450                 455                 460                 


Val Gly Pro Met Ile Thr Pro Asp Asp Gln Ala Lys Ser Ala Glu Thr 
465                 470                 475                 480 


Val Phe Ser Trp Met Glu Arg Lys Gly Lys Glu Ala Gly Gln Ile Thr 
                485                 490                 495     


Pro Trp Asn Phe Asp Glu Lys Val Asp Arg Asn Ala Ser Ala Asn Arg 
            500                 505                 510         


Phe Ile Lys Arg Met Thr Thr Lys Asp Thr Tyr Leu Ile Gly Glu Asp 
        515                 520                 525             


Val Leu Pro Asp Glu Ser Leu Leu Tyr Glu Lys Phe Lys Val Leu Asn 
    530                 535                 540                 


Glu Leu Asn Met Val Arg Val Asn Gly Lys Leu Leu Lys Val Ala Asp 
545                 550                 555                 560 


Lys Gln Ala Ile Phe Gln Asp Leu Phe Glu Asn Tyr Lys His Ile Ser 
                565                 570                 575     


Val Lys Lys Leu Gln Asn Tyr Ile Lys Ser Lys Thr Gly Leu Pro Ser 
            580                 585                 590         


Asp Pro Glu Ile Ser Gly Leu Ser Asp Pro Glu Tyr Phe Asn Asn Ser 
        595                 600                 605             


Leu Gly Thr Tyr Asn Asp Phe Lys Lys Leu Phe Gly Asn Lys Val Asp 
    610                 615                 620                 


Glu Pro Asp Leu Gln Asp Asp Phe Glu Lys Ile Val Glu Trp Ser Thr 
625                 630                 635                 640 


Val Phe Glu Asp Lys Arg Ile Leu Arg Glu Lys Leu Asn Glu Ile Thr 
                645                 650                 655     


Trp Leu Ser Asp Gln Gln Lys Asp Val Leu Glu Ser Ser Arg Tyr Gln 
            660                 665                 670         


Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gly Ile Val Asn Asp 
        675                 680                 685             


Gln Gly Glu Arg Ile Ile Asp Glu Leu Trp Asn Thr Asn Lys Asn Phe 
    690                 695                 700                 


Met Gln Ile Gln Ser Asp Asn Asp Phe Ala Lys Arg Ile His Glu Ala 
705                 710                 715                 720 


Asn Ala Asp Gln Met Lys Ala Val Asp Val Glu Asp Val Leu Ala Asp 
                725                 730                 735     


Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Val Lys 
            740                 745                 750         


Val Val Asp Asp Ile Gln Lys Ala Met Gly Gly Val Ala Pro Lys Tyr 
        755                 760                 765             


Ile Ser Ile Glu Phe Thr Arg Ser Glu Asp Arg Asn Pro Arg Arg Thr 
    770                 775                 780                 


Ile Ser Arg Gln Arg Gln Leu Glu Asn Thr Leu Lys Asp Thr Ala Lys 
785                 790                 795                 800 


Ser Leu Ala Lys Ser Ile Asn Pro Glu Leu Leu Ser Glu Leu Asp Asn 
                805                 810                 815     


Ala Ala Lys Ser Lys Lys Gly Leu Thr Asp Arg Leu Tyr Leu Tyr Phe 
            820                 825                 830         


Thr Gln Leu Gly Lys Asp Ile Tyr Thr Gly Lys Pro Ile Asn Ile Asp 
        835                 840                 845             


Glu Ile Ser Thr Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Phe Val 
    850                 855                 860                 


Lys Asp Asp Ser Leu Asn Asn Arg Val Leu Val Ser Lys Ala Ile Asn 
865                 870                 875                 880 


Asn Gly Lys Ser Asp Asn Val Pro Val Gln Leu Phe Gly Ala Lys Met 
                885                 890                 895     


Gly His Phe Trp Lys Gln Leu Ala Glu Ala Gly Leu Ile Ser Lys Arg 
            900                 905                 910         


Lys Leu Lys Asn Leu Gln Thr Asp Pro Asp Thr Ile Ser Lys Tyr Ala 
        915                 920                 925             


Met His Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile 
    930                 935                 940                 


Lys Leu Val Ala Asn Ile Leu Gly Asp Lys Tyr Arg Asn Asp Asn Thr 
945                 950                 955                 960 


Lys Ile Ile Glu Ile Thr Ala Arg Met Asn His Gln Met Arg Asp Glu 
                965                 970                 975     


Phe Gly Phe Ile Lys Asn Arg Glu Ile Asn Asp Tyr His His Ala Phe 
            980                 985                 990         


Asp Ala Tyr Leu Thr Ala Phe Leu  Gly Arg Tyr Leu Tyr  His Arg Tyr 
        995                 1000                 1005             


Ile Lys  Leu Arg Pro Tyr Phe  Val Tyr Gly Asp Phe  Lys Lys Phe 
    1010                 1015                 1020             


Lys Glu  Asp Lys Val Thr Met  Arg Asn Phe Asn Phe  Leu His Asp 
    1025                 1030                 1035             


Leu Thr  Asp Asp Thr Gln Glu  Lys Ile Ala Asp Ala  Glu Thr Gly 
    1040                 1045                 1050             


Glu Val  Ile Trp Asp Arg Glu  Asn Ser Ile Gln Gln  Leu Lys Asp 
    1055                 1060                 1065             


Val Tyr  His Tyr Lys Phe Met  Leu Ile Ser His Glu  Val Tyr Thr 
    1070                 1075                 1080             


Leu Arg  Gly Ala Met Phe Asn  Gln Thr Val Tyr Pro  Ala Ser Asp 
    1085                 1090                 1095             


Ala Gly  Lys Arg Lys Leu Ile  Pro Ile Lys Ala Asp  Arg Pro Ile 
    1100                 1105                 1110             


Asn Val  Tyr Gly Gly Tyr Ser  Gly Ser Ala Asp Ala  Tyr Met Ala 
    1115                 1120                 1125             


Ile Val  Arg Ile His Asn Lys  Lys Gly Asp Lys Tyr  Arg Val Val 
    1130                 1135                 1140             


Gly Val  Pro Met Arg Ala Arg  Asp Arg Leu Asp Ala  Ala Lys Lys 
    1145                 1150                 1155             


Val Ser  Asp Ala Asp Cys Asp  Arg Ala Leu Lys Asp  Val Leu Thr 
    1160                 1165                 1170             


Pro Gln  Leu Thr Lys Thr Lys  Lys Ser Arg Lys Thr  Gly Glu Ile 
    1175                 1180                 1185             


Thr Gln  Val Val Glu Asp Phe  Glu Ile Val Leu Gly  Lys Val Met 
    1190                 1195                 1200             


Tyr Arg  Gln Leu Met Ile Asp  Gly Asp Lys Lys Phe  Met Leu Gly 
    1205                 1210                 1215             


Ser Ser  Thr Tyr Gln Tyr Asn  Ala Lys Gln Leu Val  Leu Ser Asp 
    1220                 1225                 1230             


Gln Ser  Val Lys Thr Leu Ala  Ser Lys Gly Arg Leu  Asp Pro Leu 
    1235                 1240                 1245             


Gln Glu  Ser Met Asp Tyr Asn  Asn Val Tyr Thr Glu  Ile Leu Asp 
    1250                 1255                 1260             


Lys Val  Asn Gln Tyr Phe Ser  Leu Tyr Asp Met Asn  Lys Phe Arg 
    1265                 1270                 1275             


His Lys  Leu Asn Leu Gly Phe  Ser Lys Phe Ile Ser  Phe Pro Asn 
    1280                 1285                 1290             


His Asn  Val Phe Asp Gly Asn  Thr Lys Ala Ser Ser  Gly Lys Arg 
    1295                 1300                 1305             


Glu Ile  Leu Glu Glu Val Leu  Asn Gly Leu His Ala  Asn Pro Thr 
    1310                 1315                 1320             


Phe Gly  Asn Leu Lys Asp Ile  Gly Ile Thr Thr Pro  Phe Gly Gln 
    1325                 1330                 1335             


Leu Gln  Gln Pro Asn Gly Ile  Leu Leu Ser Asp Glu  Ala Lys Ile 
    1340                 1345                 1350             


Arg Tyr  Gln Ser Pro Thr Gly  Leu Phe Glu Arg Thr  Val Ser Leu 
    1355                 1360                 1365             


Lys Asp  Leu 
    1370     


<210>  1032
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1032

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Ser Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Met Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Arg Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Glu Phe Leu Ser Gly Lys Gln 
    530                 535                 540                 


Lys Glu Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Lys Glu Met Ile Glu Glu Arg Leu Lys Lys Tyr Ala 
625                 630                 635                 640 


Asn Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Ala Ile Gln Lys Ala Gln Val Ser Gly Gln Gly His Ser Leu 
705                 710                 715                 720 


His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Arg  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Glu Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Ala Leu Thr  Asn Glu Ser Ile Tyr  Ala Arg Gly 
    1100                 1105                 1110             


Ser Phe  Asp Lys Leu Ile Ser  Arg Lys His Arg Phe  Glu Ser Ser 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Gly Ser  Pro Thr Val Thr Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Ser Lys Val  Gln Asp Gly Lys Val  Lys Lys Ile 
    1145                 1150                 1155             


Lys Thr  Gly Lys Glu Leu Ile  Gly Ile Thr Leu Leu  Asp Lys Leu 
    1160                 1165                 1170             


Val Phe  Glu Lys Asn Pro Leu  Lys Phe Ile Glu Asp  Lys Gly Tyr 
    1175                 1180                 1185             


Gly Asn  Val Gln Ile Asp Lys  Cys Ile Lys Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Phe Glu Asn Gly  Thr Arg Arg Met Leu  Ala Ser Val 
    1205                 1210                 1215             


Met Ala  Asn Asn Asn Ser Arg  Gly Asp Leu Gln Lys  Ala Asn Glu 
    1220                 1225                 1230             


Met Phe  Leu Pro Ala Lys Leu  Val Thr Leu Leu Tyr  His Ala His 
    1235                 1240                 1245             


Lys Ile  Glu Ser Ser Lys Glu  Leu Glu His Glu Ala  Tyr Ile Leu 
    1250                 1255                 1260             


Asp His  Tyr Asn Asp Leu Tyr  Gln Leu Leu Ser Tyr  Ile Glu Arg 
    1265                 1270                 1275             


Phe Ala  Ser Leu Tyr Val Asp  Val Glu Lys Asn Ile  Ser Lys Val 
    1280                 1285                 1290             


Lys Glu  Leu Phe Ser Asn Ile  Glu Ser Tyr Ser Ile  Ser Glu Ile 
    1295                 1300                 1305             


Cys Ser  Ser Val Ile Asn Leu  Leu Thr Leu Thr Ala  Ser Gly Ala 
    1310                 1315                 1320             


Pro Ala  Asp Phe Lys Phe Leu  Gly Thr Thr Ile Pro  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Gly  Ser Pro Gln Ser Ile  Leu Ser Ser Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1355                 1360                 1365             


Gly Gly  Asp 
    1370     


<210>  1033
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1033

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Ser Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Met Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Arg Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Glu Phe Leu Ser Gly Lys Gln 
    530                 535                 540                 


Lys Glu Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Lys Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Ile Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Ala Ile Gln Lys Ala Gln Val Ser Gly Gln Gly His Ser Leu 
705                 710                 715                 720 


His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asp Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Arg  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Glu Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asn Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Ala Leu Thr  Asn Glu Ser Ile Tyr  Ala Arg Gly 
    1100                 1105                 1110             


Ser Phe  Asp Lys Leu Ile Ser  Arg Lys His Arg Phe  Glu Ser Ser 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Gly Ser  Pro Thr Val Thr Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Ser Lys Val  Gln Asp Gly Lys Val  Lys Lys Ile 
    1145                 1150                 1155             


Lys Thr  Gly Lys Glu Leu Ile  Gly Ile Thr Leu Leu  Asp Lys Leu 
    1160                 1165                 1170             


Val Phe  Glu Lys Asn Pro Leu  Lys Phe Ile Glu Asp  Lys Gly Tyr 
    1175                 1180                 1185             


Gly Asn  Val Gln Ile Asp Lys  Cys Ile Lys Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Phe Glu Asn Gly  Thr Arg Arg Met Leu  Ala Ser Val 
    1205                 1210                 1215             


Met Ala  Asn Asn Asn Ser Arg  Gly Asp Leu Gln Lys  Ala Asn Glu 
    1220                 1225                 1230             


Met Phe  Leu Pro Ala Lys Leu  Val Thr Leu Leu Tyr  His Ala His 
    1235                 1240                 1245             


Lys Ile  Glu Ser Ser Lys Glu  Leu Glu His Glu Ala  Tyr Ile Leu 
    1250                 1255                 1260             


Asp His  Tyr Asn Asp Leu Tyr  Gln Leu Leu Ser Tyr  Ile Glu Arg 
    1265                 1270                 1275             


Phe Ala  Ser Leu Tyr Val Asp  Val Glu Lys Asn Ile  Ser Lys Val 
    1280                 1285                 1290             


Lys Glu  Leu Phe Ser Asn Ile  Glu Ser Tyr Ser Ile  Ser Glu Ile 
    1295                 1300                 1305             


Cys Ser  Ser Val Ile Asn Leu  Leu Thr Leu Thr Ala  Ser Gly Ala 
    1310                 1315                 1320             


Pro Ala  Asp Phe Lys Phe Leu  Gly Thr Thr Ile Pro  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Gly  Ser Pro Gln Ser Ile  Leu Ser Ser Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1355                 1360                 1365             


Gly Gly  Asp 
    1370     


<210>  1034
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1034

Met Asn Asn Asn Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Arg Val Leu Gly Asn Thr Asp Lys Arg Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys 
            100                 105                 110         


Ser Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Thr Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ala Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Asn Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Gly Lys Ile Ser Lys Ser Val Lys Arg Glu 
    210                 215                 220                 


His Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Ser Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Glu Asp Leu Ala Ala Leu 
                325                 330                 335     


Lys Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Ala Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Leu Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Lys Ala Ser Ser Ala Glu Ser Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Phe Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile His Tyr Leu His Asn Val Asp Gly Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             


Asn His Lys Asn Gln Glu Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 


Ser Ile Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Cys Asp 
            660                 665                 670         


Lys Lys Thr Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Tyr 
        675                 680                 685             


Asn Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 


Lys Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asp Asp Leu 
705                 710                 715                 720 


Thr Gln Val Val Arg Glu Leu Ser Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Ile Met Gly 
            740                 745                 750         


Tyr Ala Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Lys Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ala Leu Lys Asn Leu Ala Pro Gly Leu Asp Ser Thr Ile Ser Lys 
785                 790                 795                 800 


Glu Asn Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp 
            820                 825                 830         


Ile Asn Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val Val Lys 
865                 870                 875                 880 


Lys Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Glu Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp 
            900                 905                 910         


Glu Arg Asp Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr 
    930                 935                 940                 


Glu Val Ser Glu Lys Asn Gln Lys Ile Arg Ser Val Lys Ile Ile Thr 
945                 950                 955                 960 


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Ala Val Val Ala Lys Ala Ile  Leu Lys Lys Tyr Pro  Lys Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr Gln Lys Tyr Asp  Leu Lys Arg 
    1010                 1015                 1020             


Tyr Ile  Ser Lys Ser Lys Asp  Pro Lys Glu Val Glu  Lys Ala Thr 
    1025                 1030                 1035             


Glu Lys  Tyr Phe Phe Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Glu 
    1040                 1045                 1050             


Glu Val  His Tyr Ala Asp Gly  Thr Ile Val Lys Arg  Glu Asn Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Lys Glu Lys 
    1070                 1075                 1080             


Asp Phe  Val Thr Ile Lys Lys  Val Leu Ser Tyr Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Arg Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu 
    1100                 1105                 1110             


Ser Ile  Leu Pro Lys Gly Asn  Ser Asp Lys Leu Ile  Pro Arg Lys 
    1115                 1120                 1125             


Thr Lys  Asp Ile Leu Trp Asp  Thr Thr Lys Tyr Gly  Gly Phe Asp 
    1130                 1135                 1140             


Ser Pro  Val Ile Ala Tyr Ser  Ile Leu Leu Ile Ala  Asp Ile Glu 
    1145                 1150                 1155             


Lys Gly  Lys Ala Lys Lys Leu  Lys Thr Val Lys Thr  Leu Val Gly 
    1160                 1165                 1170             


Ile Thr  Ile Met Glu Lys Ala  Ala Phe Glu Lys Asn  Pro Ile Thr 
    1175                 1180                 1185             


Phe Leu  Glu Asn Lys Gly Tyr  His Asn Val Arg Lys  Glu Asn Ile 
    1190                 1195                 1200             


Leu Cys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1205                 1210                 1215             


Arg Arg  Leu Leu Ala Ser Ala  Lys Glu Leu Gln Lys  Gly Asn Glu 
    1220                 1225                 1230             


Ile Val  Leu Pro Val Tyr Leu  Thr Thr Leu Leu Tyr  His Ser Lys 
    1235                 1240                 1245             


Asn Val  His Lys Leu Asp Glu  Pro Glu His Leu Glu  Tyr Ile Gln 
    1250                 1255                 1260             


Lys His  Arg Tyr Glu Phe Lys  Asp Leu Leu Asn Leu  Val Ser Glu 
    1265                 1270                 1275             


Phe Ser  Gln Lys Tyr Val Leu  Ala Glu Ala Asn Leu  Glu Lys Ile 
    1280                 1285                 1290             


Lys Ser  Leu Tyr Val Asp Asn  Glu Gln Ala Asp Ile  Glu Ile Leu 
    1295                 1300                 1305             


Ala Asn  Ser Phe Ile Asn Leu  Leu Thr Phe Thr Ala  Leu Gly Ala 
    1310                 1315                 1320             


Pro Ala  Ala Phe Lys Phe Phe  Gly Lys Asp Val Asp  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Thr  Thr Val Ser Glu Ile  Leu Asn Ala Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu 
    1355                 1360                 1365             


Gly Glu  Asp 
    1370     


<210>  1035
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1035

Met Thr Glu Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Ala Leu Leu Phe Asp Asn Gly Glu Thr Ala Glu Ala Thr Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu 
                85                  90                  95      


Ser Phe Phe Tyr Arg Leu Asp Glu Ser Phe Leu Thr Thr Asp Asp Lys 
            100                 105                 110         


Asp Phe Glu Arg His Pro Ile Phe Gly Asn Lys Ala Asp Glu Ile Lys 
        115                 120                 125             


Tyr His Gln Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser His Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn 
                165                 170                 175     


Ala Glu Asn Thr Asp Val Gln Lys Leu Phe Glu Ala Phe Val Glu Val 
            180                 185                 190         


Tyr Asp Arg Thr Phe Asp Asp Ser Asn Leu Ser Glu Ile Thr Val Asp 
        195                 200                 205             


Ala Ser Ser Ile Leu Thr Glu Lys Phe Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly 
225                 230                 235                 240 


Asn Leu Val Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Ser 
                245                 250                 255     


Phe Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Ile Gly Lys Ile Gly Asp Glu Tyr Ala 
        275                 280                 285             


Asp Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Ala Asp Asn Thr Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Lys Arg Tyr Asn Glu His Gln Val Asp Leu Lys Lys Leu 
                325                 330                 335     


Lys Glu Phe Ile Lys Asn Asn Ala Ser Asp Lys Tyr Asp Glu Ile Phe 
            340                 345                 350         


Asn Asp Lys Asp Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val 
        355                 360                 365             


Lys Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Thr Thr Leu Ser Lys Ile 
    370                 375                 380                 


Asp Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Ala 
            420                 425                 430         


Phe Leu Lys Glu Asn Gln Ala Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asn Ser Arg Phe Ala 
    450                 455                 460                 


Trp Ala Glu Tyr His Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Glu Ile Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Val Tyr Glu Thr Tyr Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Tyr Val Asn Glu Gln Gly Lys Ser Asn Phe Phe Asp Ala Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Glu His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 


Lys Asp Lys Phe Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg 
                565                 570                 575     


Ile Gln Asp Leu Ile Gly Leu Asp Lys Glu Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys Ser Phe 
        595                 600                 605             


Leu Asp Asp Lys Thr Asn Glu Thr Ile Ile Glu Asp Ile Ile Gln Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Asp Met Ile Arg Gln Arg Leu Gln Lys 
625                 630                 635                 640 


Tyr Ser Asp Ile Phe Thr Pro Gln Gln Leu Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asn Lys Glu Asn Gly Lys Ser Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Tyr Ala Asn Arg Asn Phe Met Gln Leu Ile Ser Asp Asp Thr Leu 
    690                 695                 700                 


Pro Phe Lys Gln Ile Ile Lys Asp Ala Gln Ile Ile Gly Asp Ile Asp 
705                 710                 715                 720 


Asp Val Thr Ser Val Val Arg Glu Leu Pro Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly His Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Asn Arg Gly Arg Asn Gln Ser Gln Gln Arg Leu Lys Lys 
    770                 775                 780                 


Leu Gln Asp Ser Leu Lys Glu Leu Gly Ser Asn Ile Leu Asn Glu Glu 
785                 790                 795                 800 


Lys Pro Ser Tyr Ile Glu Gly Lys Val Glu Asn Asn His Leu Gln Asp 
                805                 810                 815     


Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr Thr 
            820                 825                 830         


Gly Asp Glu Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile Asp His 
        835                 840                 845             


Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val 
    850                 855                 860                 


Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser 
865                 870                 875                 880 


Leu Asp Ile Val His Asp Arg Lys Ala Asp Trp Ile Arg Leu Tyr Lys 
                885                 890                 895     


Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu 
            900                 905                 910         


Arg Gly Gly Leu Thr Glu Asn Asp Lys Ala Gly Phe Ile Lys Arg Gln 
        915                 920                 925             


Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp 
    930                 935                 940                 


Ser Arg Phe Asn Thr Glu Arg Asp Glu Asn Asp Lys Val Ile Arg Asn 
945                 950                 955                 960 


Val Lys Val Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys 
                965                 970                 975     


Asp Phe Lys Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala 
            980                 985                 990         


His Asp Ala Tyr Leu Asn Ala Val  Val Gly Thr Ala Leu  Leu Lys Lys 
        995                 1000                 1005             


Tyr Pro  Lys Leu Ala Pro Glu  Phe Val Tyr Gly Glu  Tyr Lys Lys 
    1010                 1015                 1020             


Tyr Asp  Ile Arg Lys Phe Ile  Thr Ser Ser Gly Asp  Lys Ala Thr 
    1025                 1030                 1035             


Ala Lys  Tyr Phe Phe Tyr Ser  Asn Leu Met Asn Phe  Phe Lys Arg 
    1040                 1045                 1050             


Val Ile  Arg Tyr Ser Asn Gly  Lys Val Val Val Arg  Pro Val Ile 
    1055                 1060                 1065             


Glu Cys  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Lys Gln Thr 
    1070                 1075                 1080             


Asp Phe  Glu Lys Val Arg Arg  Val Leu Ser Tyr Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Val Glu Thr  Gln Thr Gly Gly Phe  Ser Lys Glu 
    1100                 1105                 1110             


Ser Ile  Leu Pro Lys Gly Asn  Ser Asp Lys Leu Ile  Pro Arg Lys 
    1115                 1120                 1125             


Thr Lys  Lys Phe Arg Trp Asp  Thr Pro Lys Tyr Gly  Gly Phe Asp 
    1130                 1135                 1140             


Ser Pro  Asn Ile Ala Tyr Ser  Val Phe Val Ile Ala  Asp Val Glu 
    1145                 1150                 1155             


Lys Gly  Lys Ala Lys Lys Leu  Lys Thr Val Lys Glu  Leu Val Gly 
    1160                 1165                 1170             


Ile Ser  Ile Met Glu Arg Ser  Ser Phe Glu Glu Asn  Pro Val Val 
    1175                 1180                 1185             


Phe Leu  Glu Lys Lys Gly Tyr  Gln Asn Val Gln Glu  Asp Asn Leu 
    1190                 1195                 1200             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Phe Glu  Gly Gly Arg 
    1205                 1210                 1215             


Arg Arg  Leu Leu Ala Ser Ala  Ser Glu Leu Gln Lys  Gly Asn Glu 
    1220                 1225                 1230             


Val Val  Leu Ser Arg His Leu  Val Glu Leu Leu Tyr  His Ala His 
    1235                 1240                 1245             


Arg Val  Asn Ser Phe Asn Asn  Ser Glu His Leu Lys  Tyr Val Ser 
    1250                 1255                 1260             


Glu His  Lys Lys Glu Phe Gly  Glu Val Leu Ser Cys  Val Glu Asn 
    1265                 1270                 1275             


Phe Ala  Lys Ser Tyr Val Asp  Val Glu Lys Asn Leu  Gly Lys Ile 
    1280                 1285                 1290             


Arg Ala  Val Ala Asp Lys Ile  Asp Thr Phe Ser Ile  Glu Asp Ile 
    1295                 1300                 1305             


Ser Ile  Ser Phe Val Asn Leu  Leu Thr Leu Thr Ala  Leu Gly Ala 
    1310                 1315                 1320             


Pro Ala  Asp Phe Asn Phe Leu  Gly Glu Lys Ile Pro  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Thr  Ser Thr Lys Glu Cys  Leu Asn Ala Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu 
    1355                 1360                 1365             


Gly Glu  Glu 
    1370     


<210>  1036
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1036

Met Asn Leu Arg Asn Val Glu Ser Tyr Gln Val Gly Leu Asp Ile Gly 
1               5                   10                  15      


Thr Gly Ser Val Gly Trp Ala Val Leu Asp Asp Asn Gly Asp Leu Cys 
            20                  25                  30          


Arg Phe Lys Gly Lys Pro Thr Trp Gly Ser Arg Val Phe Pro Thr Ala 
        35                  40                  45              


Glu Thr Ala Ala Glu Ala Arg Val His Arg Gly Gln Arg Arg Arg Tyr 
    50                  55                  60                  


Asp Arg Arg Arg Gln Arg Ile Asp Leu Leu Gln Arg Phe Phe Ala Asp 
65                  70                  75                  80  


Glu Val Ala Lys Val Asp Pro Glu Phe Phe Ile Arg Leu Asn Gln Ala 
                85                  90                  95      


Arg Leu His Pro Glu Asn Arg Asp Pro Ala His Ala Asp Tyr Arg Trp 
            100                 105                 110         


Pro Leu Phe Asn Gly Ser Asn Phe Thr Glu Lys Asp Tyr Tyr Arg Gln 
        115                 120                 125             


Phe Pro Thr Ile Tyr His Leu Arg Ala His Leu Leu Lys Ser Asp Glu 
    130                 135                 140                 


Pro Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu His Asn Ile Val Lys 
145                 150                 155                 160 


His Arg Gly Asn Phe Leu His Gln Asp Asn Pro Ser Leu Ser Ala Arg 
                165                 170                 175     


Asn Ala Asn Val Gly Asp Ala Val Glu Ala Leu Cys Leu Ala Leu Asp 
            180                 185                 190         


Glu Trp Cys Gly Ala His Asp Val Glu Cys Ala Cys Asp Glu Ala Ala 
        195                 200                 205             


Leu Ala Glu Ala Leu Gly Asp Ala Ser Leu Arg Pro Gly Glu Lys Arg 
    210                 215                 220                 


Glu Arg Ala Glu Ala Ala Leu Gly Val Glu Lys Ala His Lys Lys Leu 
225                 230                 235                 240 


Ala Lys Ala Val Ser Ser Ala Val Val Gly Tyr Ala Ala Glu Phe Gly 
                245                 250                 255     


His Val Phe Phe Ala Glu Ser Glu Gly Ser Lys Phe Ala Leu Ser Asn 
            260                 265                 270         


Asp Glu Lys Val Glu Ala Tyr Val Cys Pro Asp Glu Gly Arg Asp Leu 
        275                 280                 285             


Phe Asp Ala Met Arg Ala Val His Ser Ser Tyr Val Leu Met Gly Ile 
    290                 295                 300                 


Leu Ser Glu Ala Gln Gly Glu Leu Leu Ser Val Cys Lys Val Gly Glu 
305                 310                 315                 320 


Tyr Glu Arg Tyr Lys Arg Asp Leu Glu Thr Leu Lys Ser Leu Val Arg 
                325                 330                 335     


Ala Tyr Gly Pro Glu Arg Tyr Asp Glu Phe Phe Cys Gly Pro Phe Tyr 
            340                 345                 350         


Glu Gly Thr Arg Asp Tyr Asp Pro Gly Lys Ala Gln Gly Tyr Thr Lys 
        355                 360                 365             


Tyr Asn Leu Gly Ala Ser Lys Leu Ser His Glu Asp Phe Leu Lys Glu 
    370                 375                 380                 


Val Gln Lys Leu Leu Lys Asp Ile Gly Ala Gln Ala Asp Glu Arg Tyr 
385                 390                 395                 400 


Ala Asp Ile Glu Arg Ala Ile Glu Ala Gly Ala Phe Leu Arg Arg Leu 
                405                 410                 415     


Lys Thr Ser Asp Asn Gly Thr Ile Pro Tyr Gln Leu His Leu Glu Glu 
            420                 425                 430         


Met Asp Ala Ile Ile Glu Arg Gln Ala Ala Phe Tyr Pro Phe Leu Ala 
        435                 440                 445             


Gln Glu Lys Asp Lys Leu Lys Ser Leu Val Ser Phe Arg Ile Pro Tyr 
    450                 455                 460                 


Tyr Val Gly Pro Leu Thr Gln Lys Asn Ala Ala Arg Asp Gly Lys Gly 
465                 470                 475                 480 


Glu Ala Arg Phe Ala Trp Ser Ser Arg Lys Pro Gly Met Glu Asp Ala 
                485                 490                 495     


Arg Val Tyr Pro Trp Asn Trp Glu Asp Val Ile Asp Lys Asp Gln Ser 
            500                 505                 510         


Ala Glu Asp Phe Ile Arg Arg Met Thr Gly Thr Cys Thr Tyr Leu Lys 
        515                 520                 525             


Gly Glu Pro Val Leu Ala Arg Cys Ser Leu Asp Tyr Glu Leu Phe Cys 
    530                 535                 540                 


Val Leu Asn Glu Leu Ala Gly Ala Arg Trp Thr Gln Asp Gly Asp Lys 
545                 550                 555                 560 


Ser Tyr Arg Phe Asp Ala Ala Asp Arg Leu Gly Ile Val Glu Asp Leu 
                565                 570                 575     


Phe Lys Lys Arg Lys Arg Val Thr Tyr Glu Ala Ala Glu Lys Trp Leu 
            580                 585                 590         


Ala Arg Thr His Gly Pro Glu Ser Gly Lys Ser Ala Gly Gly Ala Tyr 
        595                 600                 605             


His Val Ser Gly Gly Gln Gly Glu Asp Ala Phe Glu Ser Gln Leu Ser 
    610                 615                 620                 


Ser His Tyr Asp Phe Cys Lys Ile Leu Gly Val Asp Glu Leu Ser Asp 
625                 630                 635                 640 


Ala Asp Glu Ala Met Ala Glu Asp Ile Ile Leu Trp Asn Thr Val Phe 
                645                 650                 655     


Glu Asp Arg Ser Ile Leu Lys Arg Lys Ile Lys Leu Ala Tyr Gly Asp 
            660                 665                 670         


Arg Leu Gly Asp Ala Gln Val Arg Lys Leu Cys Gln Lys Arg Tyr Thr 
        675                 680                 685             


Gly Trp Gly Arg Leu Ser Gly Glu Leu Leu Arg Gly Leu Arg Ser Ala 
    690                 695                 700                 


Thr Asp Asn Gly Pro Lys Thr Ile Met Asp Ile Leu Leu Glu Gly Asp 
705                 710                 715                 720 


Pro Asn His Ala Arg Arg Met Gly Ala Ala Met Asn Leu Met Glu Ile 
                725                 730                 735     


Leu His Asp Asp Leu Leu Glu Phe Gln Ala Lys Ile Asp Glu His Asn 
            740                 745                 750         


Arg Glu Arg Ala Leu Ala Val Gly Gly Met Ser Val Asp Glu Val Thr 
        755                 760                 765             


Gly Ser Pro Ala Leu Arg Arg Gly Ile Asn Gln Ala Leu Arg Val Val 
    770                 775                 780                 


Asp Glu Ile Ala Gly Ile Ala Gly Lys Pro Pro Ala His Ile Tyr Val 
785                 790                 795                 800 


Glu Val Thr Arg Asp Glu Gly Ala Arg Asn Lys Gly Arg Arg Thr Thr 
                805                 810                 815     


Ser Arg Tyr Arg Ala Leu Glu Ala Ala Leu Ser Ser Ile Lys Ala Gln 
            820                 825                 830         


Cys Ala Asp Val Tyr Ala Glu Leu Lys Glu Arg Asp Pro Arg Ala Leu 
        835                 840                 845             


Asp Asp Glu Arg Leu Thr Leu Tyr Phe Met Gln Cys Gly Lys Cys Met 
    850                 855                 860                 


Tyr Ser Gly Asp Ala Leu Asp Ile Asn Lys Leu Gln Thr Tyr Gln Val 
865                 870                 875                 880 


Asp His Ile Leu Pro Gln Ser Tyr Val Lys Asp Asp Ser Phe Glu Asn 
                885                 890                 895     


Lys Val Leu Val Arg Pro Asp Thr Asn Gln Arg Lys Gly Asp Thr Leu 
            900                 905                 910         


Leu Leu Asp Glu Arg Leu Ile Ala Arg Met Arg Gly Phe Trp Arg Thr 
        915                 920                 925             


Leu Arg Asp Ala Glu Leu Ile Gly Glu Lys Lys Phe Asn Asn Leu Thr 
    930                 935                 940                 


Arg Thr Ser Val Ser Asp Glu Gln Met Lys Lys Phe Val Ser Arg Gln 
945                 950                 955                 960 


Leu Val Glu Thr Gly Gln Met Val Lys Gln Val Gln Gln Leu Leu Gln 
                965                 970                 975     


Ala Arg Tyr Pro Glu Ser Glu Val Gln Pro Val Lys Ala Gly Val Ser 
            980                 985                 990         


His Gln Leu Arg Val Gly Cys Ala  Leu Ala Lys Ser Arg  Glu Ile Asn 
        995                 1000                 1005             


Asp Phe  His His Ala His Asp  Ala Tyr Leu Ala Cys  Gln Val Gly 
    1010                 1015                 1020             


Arg Phe  Val Gln Tyr Arg His  Ser Ala Val Tyr Ser  Glu Pro Val 
    1025                 1030                 1035             


Lys Met  Ala Ala Val Val Lys  Gln Phe Ile Lys Lys  Gln Ala Glu 
    1040                 1045                 1050             


Asp Tyr  Arg Arg Thr Arg Glu  Leu Pro Gly Ser Asp  Gly Phe Ile 
    1055                 1060                 1065             


Val Ser  Ser Phe Met Thr Pro  Gly Phe Asp Val Lys  Thr Gly Glu 
    1070                 1075                 1080             


Val Phe  Arg Asp Ala Trp Asp  Ala Gly Ala Glu Ile  Asp Arg Ile 
    1085                 1090                 1095             


Arg Arg  Cys Phe Asp Tyr Lys  Asp Cys Phe Ile Ser  Arg Met Pro 
    1100                 1105                 1110             


Glu Glu  Thr His Gly Ala Phe  Trp Asp Ala Thr Ile  Tyr Ser Pro 
    1115                 1120                 1125             


Arg Ala  Ala Gly Lys Thr Leu  Ser Leu Pro Leu Lys  Arg Gly Leu 
    1130                 1135                 1140             


Asp Pro  Arg Lys Tyr Gly Ser  Tyr Ser Arg Glu Gln  Phe Ala Tyr 
    1145                 1150                 1155             


Phe Phe  Val Tyr Glu Ala Val  Lys Pro Lys Lys Lys  Gln Arg Val 
    1160                 1165                 1170             


Leu Glu  Phe Ala Pro Val Pro  Val Arg Val Ala Ser  Ala Leu Ala 
    1175                 1180                 1185             


Asn Asp  Pro Asp Ala Leu Glu  Asp Tyr Ala Arg Glu  Leu Ala Glu 
    1190                 1195                 1200             


Ala Glu  Gly Leu Val Phe Glu  Arg Ile Arg Arg Arg  Lys Val Tyr 
    1205                 1210                 1215             


Lys Tyr  Gln Gln Ile Ile Val  Gly Asp Ser Arg Leu  Tyr Val Thr 
    1220                 1225                 1230             


Gly Arg  Glu Glu Val Arg Asn  Ala Arg Gln Leu Ala  Phe Ser Gln 
    1235                 1240                 1245             


Asp Glu  Thr Lys Leu Ile Cys  Arg Ile Glu Gln Gly  Glu Glu Cys 
    1250                 1255                 1260             


Glu Glu  Gly Glu Leu Leu Asp  Leu Phe Arg Ser Met  Gln Arg Lys 
    1265                 1270                 1275             


Tyr Leu  Ala Cys Ala Pro Arg  Leu Ala Arg Leu Met  Lys Val Ala 
    1280                 1285                 1290             


Glu Met  Glu Glu Gly Phe Ile  Ala Ala Thr Ala Ser  Gly Lys Arg 
    1295                 1300                 1305             


Glu Val  Leu Leu Ser Leu Thr  Ser Ile Ala Ala Ala  His Arg Asn 
    1310                 1315                 1320             


Ser Ile  Asp Leu Lys Pro Ile  Gly Arg Lys Gly Ser  Ser Gly Gln 
    1325                 1330                 1335             


Met Lys  Ile Thr Phe Ser Lys  Glu Leu Ser Thr Asn  Gly Ile Leu 
    1340                 1345                 1350             


Phe Val  Asp Ser Ser Val Thr  Gly Met Phe Glu Arg  Arg Cys Arg 
    1355                 1360                 1365             


Leu Gly  Leu 
    1370     


<210>  1037
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1037

Met Glu Asn Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Arg Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Ala Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Ile Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Asp Thr Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Ser Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Gly Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Val Leu 
                325                 330                 335     


Lys Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Thr Asp Tyr Phe Leu Glu Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Lys Asp Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Glu Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Gln Ile Val Thr Gln Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Gln Phe Leu His Asn Val Asp Gly Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             


Asp Ser Lys Asn Glu Glu Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 


Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Ile Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asp Gly Ile Cys Asp 
            660                 665                 670         


Lys Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Lys 
        675                 680                 685             


Asn Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 


Lys Glu Ile Thr Gln Lys Ala Gln Val Val Gly Lys Thr Asp Asp Val 
705                 710                 715                 720 


Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Thr Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ala Leu Lys Asn Leu Ala Ser Gly Leu Asp Ser Asn Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asn Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Lys Pro Leu Asp 
            820                 825                 830         


Ile Asn Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Val Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Leu Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln 
865                 870                 875                 880 


Lys Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Glu Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp 
            900                 905                 910         


Glu Arg Asp Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Lys 
    930                 935                 940                 


Glu Val Asn Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr 
945                 950                 955                 960 


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Arg Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Ala Val Val Ala Lys Ala Ile  Leu Lys Lys Tyr Pro  Lys Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr Gln Lys Tyr Asp  Leu Lys Arg 
    1010                 1015                 1020             


Tyr Ile  Ser Arg Ser Arg Asn  Pro Lys Glu Val Glu  Lys Ala Thr 
    1025                 1030                 1035             


Glu Lys  Tyr Phe Phe Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Glu 
    1040                 1045                 1050             


Glu Val  His Tyr Ala Asp Gly  Thr Ile Val Lys Arg  Glu Asn Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Lys Glu Lys 
    1070                 1075                 1080             


Asp Phe  Ala Thr Ile Lys Lys  Val Leu Ser Tyr Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Arg Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu 
    1100                 1105                 1110             


Ser Ile  Leu Pro Lys Gly Asn  Ser Asp Lys Leu Ile  Pro Arg Lys 
    1115                 1120                 1125             


Thr Lys  Asp Ile Leu Trp Glu  Thr Thr Lys Tyr Gly  Gly Phe Asp 
    1130                 1135                 1140             


Ser Pro  Val Ile Ala Tyr Ser  Ile Leu Leu Ile Ala  Asp Ile Glu 
    1145                 1150                 1155             


Lys Gly  Lys Ala Lys Lys Leu  Lys Thr Val Lys Thr  Leu Val Gly 
    1160                 1165                 1170             


Ile Thr  Ile Met Glu Lys Ala  Ala Phe Glu Glu Asn  Pro Ile Thr 
    1175                 1180                 1185             


Phe Leu  Glu Asn Lys Gly Tyr  His Asn Val Arg Lys  Glu Asn Ile 
    1190                 1195                 1200             


Leu Cys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1205                 1210                 1215             


Arg Arg  Leu Leu Ala Ser Ala  Lys Glu Leu Gln Lys  Gly Asn Glu 
    1220                 1225                 1230             


Ile Val  Leu Pro Val Tyr Leu  Thr Thr Leu Leu Tyr  His Ser Lys 
    1235                 1240                 1245             


Asn Val  His Lys Leu Asp Glu  Pro Glu His Leu Glu  Tyr Ile Gln 
    1250                 1255                 1260             


Lys His  Arg Asn Glu Phe Lys  Asp Leu Leu Asn Leu  Val Ser Glu 
    1265                 1270                 1275             


Phe Ser  Gln Lys Tyr Val Leu  Ala Asp Ala Asn Leu  Glu Lys Ile 
    1280                 1285                 1290             


Gln Asn  Leu Tyr Ala Asp Asn  Glu Gln Ala Asp Ile  Glu Ile Leu 
    1295                 1300                 1305             


Ala Asn  Ser Phe Ile Asn Leu  Leu Thr Phe Thr Ala  Leu Gly Ala 
    1310                 1315                 1320             


Pro Ala  Ala Phe Lys Phe Phe  Gly Lys Asp Ile Asp  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Thr  Thr Val Ser Glu Ile  Leu Asn Ala Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu 
    1355                 1360                 1365             


Gly Glu  Asp 
    1370     


<210>  1038
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1038

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Ser Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Met Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Arg Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Glu Phe Leu Ser Gly Lys Gln 
    530                 535                 540                 


Lys Glu Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Lys Glu Met Ile Glu Glu Arg Leu Lys Lys Tyr Ala 
625                 630                 635                 640 


Asn Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Ala Ile Gln Lys Ala Gln Val Ser Gly Gln Gly His Ser Leu 
705                 710                 715                 720 


His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Ile Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Thr Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Arg  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Glu Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Ala Leu Thr  Asn Glu Ser Ile Tyr  Ala Arg Gly 
    1100                 1105                 1110             


Ser Phe  Asp Lys Leu Ile Ser  Arg Lys His Arg Phe  Glu Ser Ser 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Gly Ser  Pro Thr Val Thr Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Ser Lys Val  Gln Asp Gly Lys Val  Lys Lys Ile 
    1145                 1150                 1155             


Lys Thr  Gly Lys Glu Leu Ile  Gly Met Thr Leu Leu  Asp Lys Leu 
    1160                 1165                 1170             


Val Phe  Glu Lys Asn Pro Leu  Lys Phe Ile Glu Asp  Lys Gly Tyr 
    1175                 1180                 1185             


Gly Asn  Val Gln Ile Asp Lys  Cys Ile Lys Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Phe Glu Asn Gly  Thr Arg Arg Met Leu  Ala Ser Val 
    1205                 1210                 1215             


Met Ala  Asn Asn Asn Ser Arg  Gly Asp Leu Gln Lys  Ala Asn Glu 
    1220                 1225                 1230             


Met Phe  Leu Pro Ala Lys Leu  Val Thr Leu Leu Tyr  His Ala His 
    1235                 1240                 1245             


Lys Ile  Glu Ser Ser Lys Glu  Leu Glu His Glu Ala  Tyr Ile Leu 
    1250                 1255                 1260             


Asp His  Tyr Asn Asp Leu Tyr  Gln Leu Leu Ser Tyr  Ile Glu Arg 
    1265                 1270                 1275             


Phe Ala  Ser Leu Tyr Val Asp  Val Glu Lys Asn Ile  Ser Lys Val 
    1280                 1285                 1290             


Lys Glu  Leu Phe Ser Asn Ile  Glu Ser Tyr Ser Ile  Ser Glu Ile 
    1295                 1300                 1305             


Cys Ser  Ser Val Ile Asn Leu  Leu Thr Leu Thr Ala  Ser Gly Ala 
    1310                 1315                 1320             


Pro Ala  Asp Phe Lys Phe Leu  Gly Thr Thr Ile Pro  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Gly  Ser Pro Gln Ser Ile  Leu Ser Ser Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1355                 1360                 1365             


Gly Gly  Asp 
    1370     


<210>  1039
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1039

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Ser Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Arg Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Lys Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Ile Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Ala Ile Gln Lys Ala Gln Val Ser Gly Gln Gly His Ser Leu 
705                 710                 715                 720 


His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Arg  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Glu Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Ala Leu Thr  Asn Glu Ser Ile Tyr  Ala Arg Gly 
    1100                 1105                 1110             


Ser Phe  Asp Lys Leu Ile Ser  Arg Lys His Arg Phe  Glu Ser Ser 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Gly Ser  Pro Thr Val Thr Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Ser Lys Val  Gln Asp Gly Lys Val  Lys Lys Ile 
    1145                 1150                 1155             


Lys Thr  Gly Lys Glu Leu Ile  Gly Ile Thr Leu Leu  Asp Lys Leu 
    1160                 1165                 1170             


Val Phe  Glu Lys Asn Pro Leu  Lys Phe Ile Glu Asp  Lys Gly Tyr 
    1175                 1180                 1185             


Gly Asn  Val Gln Ile Asp Lys  Cys Ile Lys Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Phe Glu Asn Gly  Thr Arg Arg Met Leu  Ala Ser Val 
    1205                 1210                 1215             


Met Ala  Asn Asn Asn Ser Arg  Gly Asp Leu Gln Lys  Ala Asn Glu 
    1220                 1225                 1230             


Met Phe  Leu Pro Ala Lys Leu  Val Thr Leu Leu Tyr  His Ala His 
    1235                 1240                 1245             


Lys Ile  Glu Ser Ser Lys Glu  Leu Glu His Glu Ala  Tyr Ile Leu 
    1250                 1255                 1260             


Asp His  Tyr Asn Asp Leu Tyr  Gln Leu Leu Ser Tyr  Ile Glu Arg 
    1265                 1270                 1275             


Phe Ala  Ser Leu Tyr Val Asp  Val Glu Lys Asn Ile  Ser Lys Val 
    1280                 1285                 1290             


Lys Glu  Leu Phe Ser Asn Ile  Glu Ser Tyr Ser Ile  Ser Glu Ile 
    1295                 1300                 1305             


Cys Ser  Ser Val Ile Asn Leu  Leu Thr Leu Thr Ala  Ser Gly Ala 
    1310                 1315                 1320             


Pro Ala  Asp Phe Lys Phe Leu  Gly Thr Thr Ile Pro  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Gly  Ser Pro Gln Ser Ile  Leu Ser Ser Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1355                 1360                 1365             


Gly Gly  Asp 
    1370     


<210>  1040
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1040

Met Glu Asn Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Ala Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ser Glu Glu Ile Gly Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Ile Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Lys 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ala Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asn Asn Thr Phe Glu Gly Asn Ser Leu Ser Gly Gln Asn Val Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Arg Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asn Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ser Leu 
                325                 330                 335     


Lys Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Glu Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Thr Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Gln Ile Val Thr Gln Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Gln Phe Leu His Asn Val Asp Gly Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Met Asp 
        595                 600                 605             


Asp Ala Lys Asn Glu Ala Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 


Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Ser Asp 
            660                 665                 670         


Lys Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Glu 
        675                 680                 685             


Ile Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 


Lys Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asn Asp Val 
705                 710                 715                 720 


Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Ala Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ser Leu Lys Ile Leu Ala Ser Gly Leu Asn Ser Lys Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp 
            820                 825                 830         


Ile Asn Gln Leu Ser Ser Cys Asp Ile Asp His Ile Ile Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val Val Asp 
865                 870                 875                 880 


Lys Met Lys Val Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Tyr Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asn 
            900                 905                 910         


Glu Leu Asp Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Lys 
    930                 935                 940                 


Glu Val Thr Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr 
945                 950                 955                 960 


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Ala Val Val Ala Lys Ala Ile  Leu Lys Lys Tyr Pro  Lys Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr Gln Lys Tyr Asp  Leu Lys Arg 
    1010                 1015                 1020             


Tyr Ile  Ser Arg Ser Lys Asp  Pro Lys Glu Ile Glu  Lys Ala Thr 
    1025                 1030                 1035             


Glu Lys  Tyr Phe Phe Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Glu 
    1040                 1045                 1050             


Glu Val  His Tyr Ala Asp Gly  Thr Ile Val Lys Arg  Glu Asn Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Lys Glu Lys 
    1070                 1075                 1080             


Asp Phe  Ala Thr Ile Lys Lys  Val Leu Ser Leu Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Arg Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu 
    1100                 1105                 1110             


Ser Ile  Leu Pro Lys Gly Asn  Ser Asp Lys Leu Ile  Pro Arg Lys 
    1115                 1120                 1125             


Thr Lys  Asp Ile Leu Trp Asp  Thr Thr Lys Tyr Gly  Gly Phe Asp 
    1130                 1135                 1140             


Ser Pro  Val Ile Ala Tyr Ser  Ile Leu Leu Ile Ala  Asp Ile Glu 
    1145                 1150                 1155             


Lys Gly  Lys Ala Lys Lys Leu  Lys Thr Val Lys Thr  Leu Val Gly 
    1160                 1165                 1170             


Ile Thr  Ile Met Glu Lys Asp  Ala Phe Glu Lys Asn  Pro Ile Ala 
    1175                 1180                 1185             


Phe Leu  Glu Asn Lys Gly Tyr  His Asn Val Cys Lys  Glu Asn Ile 
    1190                 1195                 1200             


Leu Cys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1205                 1210                 1215             


Arg Arg  Leu Leu Ala Ser Ala  Lys Glu Leu Gln Lys  Cys Asn Glu 
    1220                 1225                 1230             


Ile Val  Leu Pro Val Tyr Leu  Thr Thr Leu Leu Tyr  His Ser Lys 
    1235                 1240                 1245             


Asn Val  His Lys Leu Asp Glu  Pro Gly His Leu Glu  Tyr Ile Gln 
    1250                 1255                 1260             


Lys His  Arg Asn Glu Phe Lys  Asp Leu Leu Asn Leu  Val Ser Glu 
    1265                 1270                 1275             


Phe Ser  Gln Lys Tyr Val Leu  Ala Asp Ala Asn Leu  Glu Lys Ile 
    1280                 1285                 1290             


Lys Asn  Leu Tyr Ala Asp Asn  Glu Gln Ala Asp Ile  Glu Ile Leu 
    1295                 1300                 1305             


Ala Asn  Ser Phe Ile Asn Leu  Leu Thr Phe Thr Ala  Leu Gly Ala 
    1310                 1315                 1320             


Pro Ala  Ala Phe Lys Phe Phe  Gly Lys Asp Val Asp  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Thr  Thr Val Ser Glu Ile  Leu Asn Ala Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu 
    1355                 1360                 1365             


Gly Glu  Asp 
    1370     


<210>  1041
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1041

Met Lys Val Asn Asn Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Trp Val Ala Ile Gly Glu Asp Gly Lys Pro Leu Arg Ile Lys 
            20                  25                  30          


Gly Lys Thr Ala Ile Gly Ala Arg Leu Phe Gln Glu Gly Asn Pro Ala 
        35                  40                  45              


Ala Asp Arg Arg Met Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Lys Leu Leu Glu Glu Ile Phe Asp Pro Tyr Ile Thr 
65                  70                  75                  80  


Pro Val Asp Ser Thr Phe Phe Ala Arg Leu Lys Gln Ser Asn Leu Ser 
                85                  90                  95      


Pro Lys Asp Ser Arg Lys Glu Phe Lys Gly Ser Met Leu Phe Pro Asp 
            100                 105                 110         


Leu Thr Asp Met Gln Tyr His Lys Asp Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg His Ala Leu Met Thr Gln Asp Glu Lys Phe Asp Ile Arg Met Val 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn 
145                 150                 155                 160 


Ser Thr Pro Val Asp Ser Phe Lys Ala Ser Lys Val Asn Phe Val Asp 
                165                 170                 175     


Gln Phe Lys Lys Leu Asn Glu Leu Tyr Thr Ala Ile Asn Pro Glu Glu 
            180                 185                 190         


Ser Phe Gln Ile Asn Leu Ala Asn Ser Glu Asp Ile Gly His Gln Phe 
        195                 200                 205             


Leu Asp Pro Ser Ile Arg Lys Phe Asp Lys Lys Lys Gln Ile Pro Lys 
    210                 215                 220                 


Ile Val Pro Val Ser Val Asp Asp Lys Ala Thr Asp Lys Ile Asn Gly 
225                 230                 235                 240 


Lys Ile Ala Ser Glu Ile Ile Asn Ala Ile Leu Gly Tyr Lys Ser Lys 
                245                 250                 255     


Leu Asp Val Val Val Gln Cys Thr Pro Val Asp Ser Lys Ser Trp Ala 
            260                 265                 270         


Leu Lys Phe Asp Glu Glu Asp Ile Asp Ala Lys Leu Gln Lys Ile Leu 
        275                 280                 285             


Pro Glu Met Asp Glu Asn Gln Gln Ser Ile Ile Ala Ile Leu Gln Asn 
    290                 295                 300                 


Leu Tyr Ser Gln Val Thr Leu Asn Gln Ile Val Pro Asn Gly Met Ser 
305                 310                 315                 320 


Leu Ser Glu Ser Met Ile Glu Lys Tyr Asn Asp His His Asp His Leu 
                325                 330                 335     


Lys Leu Tyr Lys Lys Ile Ile Asp Gln Leu Ala Asp Pro Lys Lys Lys 
            340                 345                 350         


Ala Ala Leu Lys Lys Ala Tyr Ser Gln Tyr Val Gly Asp Asp Gly Lys 
        355                 360                 365             


Val Ile Glu Gln Ala Asp Phe Trp Ser Ser Val Lys Lys Asn Leu Asp 
    370                 375                 380                 


Asp Ser Asp Leu Ser Lys Gln Ile Met Asp Leu Ile Asp Ala Glu Lys 
385                 390                 395                 400 


Phe Met Pro Lys Gln Arg Thr Ser Gln Asn Gly Val Ile Pro His Gln 
                405                 410                 415     


Leu His Gln Arg Glu Leu Asp Glu Ile Ile Glu His Gln Ser Lys Tyr 
            420                 425                 430         


Tyr Pro Trp Leu Ala Glu Ile Asn Pro Asn Lys His Asp Leu His Leu 
        435                 440                 445             


Ala Lys Tyr Lys Ile Glu Glu Leu Val Ala Phe Arg Val Pro Tyr Tyr 
    450                 455                 460                 


Val Gly Pro Met Ile Thr Pro Asp Asp Gln Ala Lys Ser Ala Glu Thr 
465                 470                 475                 480 


Val Phe Ser Trp Met Glu Arg Lys Gly Lys Glu Ala Gly Gln Ile Thr 
                485                 490                 495     


Pro Trp Asn Phe Asp Glu Lys Val Asp Arg Asn Ala Ser Ala Asn Arg 
            500                 505                 510         


Phe Ile Lys Arg Met Thr Thr Lys Asp Thr Tyr Leu Ile Gly Glu Asp 
        515                 520                 525             


Val Leu Pro Asp Glu Ser Leu Leu Tyr Glu Lys Phe Lys Val Leu Asn 
    530                 535                 540                 


Glu Leu Asn Met Val Arg Val Asn Gly Lys Leu Leu Lys Val Ala Asp 
545                 550                 555                 560 


Lys Gln Ala Ile Phe Gln Asp Leu Phe Glu Asn Tyr Lys His Ile Ser 
                565                 570                 575     


Val Lys Lys Leu Gln Asn Tyr Ile Lys Ser Lys Thr Gly Leu Pro Ser 
            580                 585                 590         


Asp Pro Glu Ile Ser Gly Leu Ser Asp Pro Glu Tyr Phe Asn Asn Ser 
        595                 600                 605             


Leu Gly Thr Tyr Asn Asp Phe Lys Lys Leu Phe Gly Asn Lys Val Asp 
    610                 615                 620                 


Glu Pro Asp Leu Gln Asp Asp Phe Glu Lys Ile Val Glu Trp Ser Thr 
625                 630                 635                 640 


Val Phe Glu Asp Lys Arg Ile Leu Arg Glu Lys Leu Asn Glu Ile Thr 
                645                 650                 655     


Trp Leu Ser Asp Gln Gln Lys Asp Val Leu Glu Ser Ser Arg Tyr Gln 
            660                 665                 670         


Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gly Ile Val Asn Asp 
        675                 680                 685             


Gln Gly Glu Arg Ile Ile Asp Glu Leu Trp Asn Thr Asn Lys Asn Phe 
    690                 695                 700                 


Met Gln Ile Gln Ser Asp Asn Asp Phe Ala Lys Arg Ile His Glu Ala 
705                 710                 715                 720 


Asn Ala Asp Gln Met Lys Ala Val Asp Val Glu Asp Val Leu Ala Asp 
                725                 730                 735     


Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Val Lys 
            740                 745                 750         


Val Val Asp Asp Ile Gln Lys Ala Met Gly Gly Val Ala Pro Lys Tyr 
        755                 760                 765             


Ile Ser Ile Glu Phe Thr Arg Ser Glu Asp Arg Asn Pro Arg Arg Thr 
    770                 775                 780                 


Ile Ser Arg Gln Arg Gln Leu Glu Asn Thr Leu Lys Asp Thr Ala Lys 
785                 790                 795                 800 


Ser Leu Ala Lys Ser Ile Asn Pro Glu Leu Leu Ser Glu Leu Asp Asn 
                805                 810                 815     


Ala Ala Lys Ser Lys Lys Gly Leu Ile Asp Arg Leu Tyr Leu Tyr Phe 
            820                 825                 830         


Thr Gln Leu Gly Lys Asp Ile Tyr Thr Gly Lys Pro Ile Asn Ile Asp 
        835                 840                 845             


Glu Ile Ser Thr Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Phe Val 
    850                 855                 860                 


Lys Asp Asp Ser Leu Asn Asn Arg Val Leu Val Ser Lys Ala Ile Asn 
865                 870                 875                 880 


Asn Gly Lys Ser Asp Asn Val Pro Val Gln Leu Phe Gly Ala Lys Met 
                885                 890                 895     


Gly His Phe Trp Lys Gln Leu Ala Glu Ala Gly Leu Ile Ser Lys Arg 
            900                 905                 910         


Lys Leu Lys Asn Leu Gln Thr Asp Pro Asp Thr Ile Ser Lys Tyr Ala 
        915                 920                 925             


Met His Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile 
    930                 935                 940                 


Lys Leu Val Ala Asn Ile Leu Gly Asp Lys Tyr Arg Asn Asp Asp Thr 
945                 950                 955                 960 


Lys Ile Ile Glu Ile Thr Ala Arg Met Asn His Gln Met Arg Asp Glu 
                965                 970                 975     


Phe Gly Phe Ile Lys Asn Arg Glu Ile Asn Asp Tyr His His Ala Phe 
            980                 985                 990         


Asp Ala Tyr Leu Thr Ala Phe Leu  Gly Arg Tyr Leu Tyr  His Arg Tyr 
        995                 1000                 1005             


Ile Lys  Leu Arg Pro Tyr Phe  Val Tyr Gly Asp Phe  Lys Lys Phe 
    1010                 1015                 1020             


Lys Glu  Asp Lys Val Thr Met  Arg Asn Phe Asn Phe  Leu His Asp 
    1025                 1030                 1035             


Leu Thr  Asp Asp Thr Gln Glu  Lys Ile Ala Asp Ala  Glu Thr Gly 
    1040                 1045                 1050             


Glu Val  Ile Trp Asp Arg Glu  Asn Ser Ile Gln Gln  Leu Lys Asp 
    1055                 1060                 1065             


Val Tyr  His Tyr Lys Phe Met  Leu Ile Ser His Glu  Val Tyr Thr 
    1070                 1075                 1080             


Leu Arg  Gly Ala Met Phe Asn  Gln Thr Val Tyr Pro  Ala Ser Asp 
    1085                 1090                 1095             


Ala Gly  Lys Arg Lys Leu Ile  Pro Ile Lys Ala Asp  Arg Pro Ile 
    1100                 1105                 1110             


Asn Val  Tyr Gly Gly Tyr Ser  Gly Ser Ala Asp Ala  Tyr Met Ala 
    1115                 1120                 1125             


Ile Val  Arg Ile His Asn Lys  Lys Gly Asp Lys Tyr  Arg Val Val 
    1130                 1135                 1140             


Gly Val  Pro Met Arg Ala Arg  Asp Arg Leu Asp Ala  Ala Lys Lys 
    1145                 1150                 1155             


Val Ser  Asp Ala Asp Tyr Asp  Arg Ala Leu Lys Asp  Val Leu Thr 
    1160                 1165                 1170             


Pro Gln  Leu Thr Lys Thr Lys  Lys Ser Arg Lys Thr  Gly Glu Ile 
    1175                 1180                 1185             


Thr Gln  Val Val Glu Asp Phe  Glu Ile Val Leu Gly  Lys Val Met 
    1190                 1195                 1200             


Tyr Arg  Gln Leu Met Ile Asp  Gly Asp Lys Lys Phe  Met Leu Gly 
    1205                 1210                 1215             


Ser Ser  Thr Tyr Gln Tyr Asn  Ala Lys Gln Leu Val  Leu Ser Asp 
    1220                 1225                 1230             


Gln Ser  Val Lys Thr Leu Ala  Ser Lys Gly Arg Leu  Asp Pro Leu 
    1235                 1240                 1245             


Gln Glu  Ser Met Asp Tyr Asn  Asn Val Tyr Thr Glu  Ile Leu Asp 
    1250                 1255                 1260             


Lys Val  Asn Gln Tyr Phe Ser  Leu Tyr Asp Met Asn  Lys Phe Arg 
    1265                 1270                 1275             


His Lys  Leu Asn Leu Gly Phe  Ser Lys Phe Ile Ser  Phe Pro Asn 
    1280                 1285                 1290             


His Asn  Val Phe Asp Gly Asn  Thr Lys Ala Ser Ser  Gly Lys Arg 
    1295                 1300                 1305             


Glu Ile  Leu Glu Glu Val Leu  Asn Gly Leu His Ala  Asn Pro Thr 
    1310                 1315                 1320             


Phe Gly  Asn Leu Lys Asp Ile  Gly Ile Thr Thr Pro  Phe Gly Gln 
    1325                 1330                 1335             


Leu Gln  Gln Pro Asn Gly Ile  Leu Leu Ser Asp Glu  Ala Lys Ile 
    1340                 1345                 1350             


Arg Tyr  Gln Ser Pro Thr Gly  Leu Phe Glu Arg Thr  Val Ser Leu 
    1355                 1360                 1365             


Lys Asp  Leu 
    1370     


<210>  1042
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1042

Met Lys Val Asn Asn Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Trp Val Ala Ile Gly Lys Asp Gly Lys Pro Leu Arg Val Lys 
            20                  25                  30          


Gly Lys Thr Ala Ile Gly Ala Arg Leu Phe Gln Glu Gly Asn Pro Ala 
        35                  40                  45              


Ala Asp Arg Arg Met Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Lys Leu Leu Glu Glu Ile Phe Asp Pro Tyr Ile Thr 
65                  70                  75                  80  


Pro Val Asp Ser Thr Phe Phe Ala Arg Leu Lys Gln Ser Asn Leu Ser 
                85                  90                  95      


Pro Lys Asp Ser Arg Lys Glu Phe Lys Gly Ser Met Leu Phe Pro Asp 
            100                 105                 110         


Leu Thr Asp Met Gln Tyr His Lys Asn Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg His Ala Leu Met Thr Gln Asp Lys Lys Phe Asp Ile Arg Met Val 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn 
145                 150                 155                 160 


Ser Thr Pro Val Asp Ser Phe Lys Ala Ser Lys Val Asp Phe Val Asp 
                165                 170                 175     


Gln Phe Lys Lys Leu Asn Glu Leu Tyr Ala Ala Ile Asn Pro Glu Glu 
            180                 185                 190         


Ser Phe Lys Ile Asn Leu Ala Asn Ser Glu Asp Ile Gly His Gln Phe 
        195                 200                 205             


Leu Asp Pro Ser Ile Arg Lys Phe Asp Lys Lys Lys Gln Ile Pro Lys 
    210                 215                 220                 


Ile Val Pro Val Met Met Asn Asp Lys Val Thr Asp Arg Leu Asn Gly 
225                 230                 235                 240 


Lys Ile Ala Ser Glu Ile Ile His Ala Ile Leu Gly Tyr Lys Ala Lys 
                245                 250                 255     


Leu Asp Val Val Leu Gln Cys Thr Pro Val Asp Ser Lys Pro Trp Ala 
            260                 265                 270         


Leu Lys Phe Asp Asp Glu Asp Ile Asp Ala Lys Leu Glu Lys Ile Leu 
        275                 280                 285             


Pro Glu Met Asp Glu Asn Gln Gln Ser Ile Val Ala Ile Leu Gln Asn 
    290                 295                 300                 


Leu Tyr Ser Gln Val Thr Leu Asn Gln Ile Val Pro Asn Gly Met Ser 
305                 310                 315                 320 


Leu Ser Glu Ser Met Ile Glu Lys Tyr Asn Asp His His Asp His Leu 
                325                 330                 335     


Lys Leu Tyr Lys Lys Leu Ile Asp Gln Leu Ala Asp Pro Lys Lys Lys 
            340                 345                 350         


Ala Val Leu Lys Lys Ala Tyr Ser Gln Tyr Val Gly Asp Asp Gly Lys 
        355                 360                 365             


Val Ile Glu Gln Ala Glu Phe Trp Ser Ser Val Lys Lys Asn Leu Asp 
    370                 375                 380                 


Asp Ser Glu Leu Ser Lys Gln Ile Met Asp Leu Ile Asp Ala Glu Lys 
385                 390                 395                 400 


Phe Met Pro Lys Gln Arg Thr Ser Gln Asn Gly Val Ile Pro His Gln 
                405                 410                 415     


Leu His Gln Arg Glu Leu Asp Glu Ile Ile Glu His Gln Ser Lys Tyr 
            420                 425                 430         


Tyr Pro Trp Leu Val Glu Ile Asn Pro Asn Lys His Asp Leu His Leu 
        435                 440                 445             


Ala Lys Tyr Lys Ile Glu Gln Leu Val Ala Phe Arg Val Pro Tyr Tyr 
    450                 455                 460                 


Val Gly Pro Met Ile Thr Pro Lys Asp Gln Ala Glu Ser Ala Glu Thr 
465                 470                 475                 480 


Val Phe Ser Trp Met Glu Arg Lys Gly Thr Glu Thr Gly Gln Ile Thr 
                485                 490                 495     


Pro Trp Asn Phe Asp Glu Lys Val Asp Arg Lys Ala Ser Ala Asn Arg 
            500                 505                 510         


Phe Ile Lys Arg Met Thr Thr Lys Asp Thr Tyr Leu Ile Gly Glu Asp 
        515                 520                 525             


Val Leu Pro Asp Glu Ser Leu Leu Tyr Glu Lys Phe Lys Val Leu Asn 
    530                 535                 540                 


Glu Leu Asn Met Val Arg Val Asn Gly Lys Leu Leu Lys Val Ala Asp 
545                 550                 555                 560 


Lys Gln Ala Ile Phe Gln Asp Leu Phe Glu Asn Tyr Lys His Val Ser 
                565                 570                 575     


Val Lys Lys Leu Gln Asn Tyr Ile Lys Ala Lys Thr Gly Leu Pro Ser 
            580                 585                 590         


Asp Pro Glu Ile Ser Gly Leu Ser Asp Pro Glu His Phe Asn Asn Ser 
        595                 600                 605             


Leu Gly Thr Tyr Asn Asp Phe Lys Lys Leu Phe Gly Ser Lys Val Asp 
    610                 615                 620                 


Glu Pro Asp Leu Gln Asp Asp Phe Glu Lys Ile Val Glu Trp Ser Thr 
625                 630                 635                 640 


Val Phe Glu Asp Lys Lys Ile Leu Arg Glu Lys Leu Asn Glu Ile Thr 
                645                 650                 655     


Trp Leu Ser Asp Gln Gln Lys Asp Val Leu Glu Ser Ser Arg Tyr Gln 
            660                 665                 670         


Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gly Ile Val Asn Asp 
        675                 680                 685             


Gln Gly Glu Arg Ile Ile Asp Lys Leu Trp Asn Thr Asn Lys Asn Phe 
    690                 695                 700                 


Met Gln Ile Gln Ser Asp Asp Asp Phe Ala Lys Arg Ile His Glu Ala 
705                 710                 715                 720 


Asn Ala Asp Gln Met Gln Ala Val Asp Val Glu Asp Val Leu Ala Asp 
                725                 730                 735     


Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Val Lys 
            740                 745                 750         


Val Val Asp Asp Ile Gln Lys Ala Met Gly Gly Val Ala Pro Lys Tyr 
        755                 760                 765             


Ile Ser Ile Glu Phe Thr Arg Ser Glu Asp Arg Asn Pro Arg Arg Thr 
    770                 775                 780                 


Ile Ser Arg Gln Arg Gln Leu Glu Asn Thr Leu Lys Asp Thr Ala Lys 
785                 790                 795                 800 


Ser Leu Ala Lys Ser Ile Asn Pro Glu Leu Leu Ser Glu Leu Asp Asn 
                805                 810                 815     


Ala Ala Lys Ser Lys Lys Gly Leu Thr Asp Arg Leu Tyr Leu Tyr Phe 
            820                 825                 830         


Thr Gln Leu Gly Lys Asp Ile Tyr Thr Gly Glu Pro Ile Asn Ile Asp 
        835                 840                 845             


Glu Leu Asn Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Phe Ile 
    850                 855                 860                 


Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val Leu Thr Ala Val Asn 
865                 870                 875                 880 


Asn Gly Lys Ser Asp Asn Val Pro Leu Arg Met Phe Gly Ala Lys Met 
                885                 890                 895     


Gly His Phe Trp Lys Gln Leu Ala Glu Ala Gly Leu Ile Ser Lys Arg 
            900                 905                 910         


Lys Leu Lys Asn Leu Gln Thr Asp Pro Asp Thr Ile Ser Lys Tyr Ala 
        915                 920                 925             


Met His Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile 
    930                 935                 940                 


Lys Leu Val Ala Asn Ile Leu Gly Asp Lys Tyr Arg Asn Asp Asp Thr 
945                 950                 955                 960 


Lys Ile Ile Glu Ile Thr Ala Arg Met Asn His Gln Met Arg Asp Glu 
                965                 970                 975     


Phe Gly Phe Ile Lys Asn Arg Glu Ile Asn Asp Tyr His His Ala Phe 
            980                 985                 990         


Asp Ala Tyr Leu Thr Ala Phe Leu  Gly Arg Tyr Leu Tyr  His Arg Tyr 
        995                 1000                 1005             


Ile Lys  Leu Arg Pro Tyr Phe  Val Tyr Gly Asp Phe  Lys Lys Phe 
    1010                 1015                 1020             


Arg Glu  Asp Lys Val Thr Met  Arg Asn Phe Asn Phe  Leu His Asp 
    1025                 1030                 1035             


Leu Thr  Asp Asp Thr Gln Glu  Lys Ile Ala Asp Ala  Glu Thr Gly 
    1040                 1045                 1050             


Glu Val  Ile Trp Asp Arg Glu  Asn Ser Ile Gln Gln  Leu Lys Asp 
    1055                 1060                 1065             


Val Tyr  His Tyr Lys Phe Met  Leu Ile Ser His Glu  Val Tyr Thr 
    1070                 1075                 1080             


Leu Arg  Gly Ala Met Phe Asn  Gln Thr Val Tyr Pro  Ala Ser Asp 
    1085                 1090                 1095             


Ala Gly  Lys Arg Lys Leu Ile  Pro Val Lys Ala Asp  Arg Pro Val 
    1100                 1105                 1110             


Asn Val  Tyr Gly Gly Tyr Ser  Gly Ser Ala Asp Ala  Tyr Met Ala 
    1115                 1120                 1125             


Ile Val  Arg Ile His Asn Lys  Lys Gly Asp Lys Tyr  Arg Val Val 
    1130                 1135                 1140             


Gly Val  Pro Met Arg Ala Leu  Asp Arg Leu Asp Ala  Ala Lys Asn 
    1145                 1150                 1155             


Val Ser  Asp Ala Asp Phe Asp  Arg Ala Leu Lys Asp  Val Leu Ala 
    1160                 1165                 1170             


Pro Gln  Leu Thr Lys Thr Lys  Lys Ser Arg Lys Thr  Gly Glu Ile 
    1175                 1180                 1185             


Thr Gln  Val Ile Glu Asp Phe  Glu Ile Val Leu Gly  Lys Val Met 
    1190                 1195                 1200             


Tyr Arg  Gln Leu Met Ile Asp  Gly Asp Lys Lys Phe  Met Leu Gly 
    1205                 1210                 1215             


Ser Ser  Thr Tyr Gln Tyr Asn  Ala Lys Gln Leu Val  Leu Ser Asp 
    1220                 1225                 1230             


Gln Ser  Val Lys Thr Leu Ala  Ser Lys Gly Arg Leu  Asp Pro Leu 
    1235                 1240                 1245             


Gln Glu  Ser Met Asp Tyr Asn  Asn Val Tyr Thr Glu  Ile Leu Asp 
    1250                 1255                 1260             


Lys Val  Asn Gln Tyr Phe Ser  Leu Tyr Asp Met Asn  Lys Phe Arg 
    1265                 1270                 1275             


His Lys  Leu Asn Leu Gly Phe  Ser Lys Phe Ile Ser  Phe Pro Asn 
    1280                 1285                 1290             


His Asn  Val Leu Asp Gly Asn  Thr Lys Val Ser Ser  Gly Lys Arg 
    1295                 1300                 1305             


Glu Ile  Leu Gln Glu Ile Leu  Asn Gly Leu His Ala  Asn Pro Thr 
    1310                 1315                 1320             


Phe Gly  Asn Leu Lys Asp Val  Gly Ile Thr Thr Pro  Phe Gly Gln 
    1325                 1330                 1335             


Leu Gln  Gln Pro Asn Gly Ile  Leu Leu Ser Asp Glu  Thr Lys Ile 
    1340                 1345                 1350             


Arg Tyr  Gln Ser Pro Thr Gly  Leu Phe Glu Arg Thr  Val Ser Leu 
    1355                 1360                 1365             


Lys Asp  Leu 
    1370     


<210>  1043
<211>  1371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1043

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Thr Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Lys Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Ile Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Ala Ile Gln Lys Ala Gln Val Ser Gly Gln Gly His Ser Leu 
705                 710                 715                 720 


His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asp Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Arg  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Glu Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Ala Leu Thr  Asn Glu Ser Ile Tyr  Ala Arg Gly 
    1100                 1105                 1110             


Ser Phe  Asp Lys Leu Ile Ser  Arg Lys His Arg Phe  Glu Ser Ser 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Gly Ser  Pro Thr Val Thr Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Ser Lys Val  Gln Asp Gly Lys Val  Lys Lys Ile 
    1145                 1150                 1155             


Lys Thr  Gly Lys Glu Leu Ile  Gly Ile Thr Leu Leu  Asp Lys Leu 
    1160                 1165                 1170             


Val Phe  Glu Lys Asn Pro Leu  Lys Phe Ile Glu Asp  Lys Gly Tyr 
    1175                 1180                 1185             


Gly Asn  Val Gln Ile Asp Lys  Cys Ile Lys Leu Pro  Lys Tyr Ser 
    1190                 1195                 1200             


Leu Phe  Glu Phe Glu Asn Gly  Thr Arg Arg Met Leu  Ala Ser Val 
    1205                 1210                 1215             


Met Ala  Asn Asn Asn Ser Arg  Gly Asp Leu Gln Lys  Ala Asn Glu 
    1220                 1225                 1230             


Met Phe  Leu Pro Ala Lys Leu  Val Thr Leu Leu Tyr  His Ala His 
    1235                 1240                 1245             


Lys Ile  Glu Ser Ser Lys Glu  Leu Glu His Glu Ala  Tyr Ile Leu 
    1250                 1255                 1260             


Asp His  Tyr Asn Asp Leu Tyr  Gln Leu Leu Ser Tyr  Ile Glu Arg 
    1265                 1270                 1275             


Phe Ala  Ser Leu Tyr Val Asp  Val Glu Lys Asn Ile  Ser Lys Val 
    1280                 1285                 1290             


Lys Glu  Leu Phe Ser Asn Ile  Glu Ser Tyr Ser Ile  Ser Glu Ile 
    1295                 1300                 1305             


Cys Ser  Ser Val Ile Asn Leu  Leu Thr Leu Thr Ala  Ser Gly Ala 
    1310                 1315                 1320             


Pro Ala  Asp Phe Lys Phe Leu  Gly Thr Thr Ile Pro  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Gly  Ser Pro Gln Ser Ile  Leu Ser Ser Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1355                 1360                 1365             


Gly Ser  Asp 
    1370     


<210>  1044
<211>  1372
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1044

Met Asn Asn Glu Tyr Phe Val Gly Phe Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Ala Ala Val Thr Asp Glu Lys Tyr His Val Leu Lys Phe Lys Gly 
            20                  25                  30          


Asn Ala Met Trp Thr Ser Ala Val Phe Glu Ser Ala Gln Gln Ser Ala 
        35                  40                  45              


Asp Arg Arg Leu Asn Arg Thr Ala Arg Arg Arg Leu Asp Arg Ser Gln 
    50                  55                  60                  


Gln Arg Arg Asp Leu Leu Gln Glu Leu Ile Ala Pro Ala Ile Thr Pro 
65                  70                  75                  80  


Val Asp Pro Arg Phe Phe Asp Arg Ile Arg Glu Ser Ala Leu Trp Ala 
                85                  90                  95      


Glu Asp Lys Thr Thr Gly Ser Arg Phe Thr Tyr Phe Ser Asp Glu Gly 
            100                 105                 110         


Tyr Thr Asp Lys Gln Tyr His Lys Glu Tyr Pro Thr Ile His His Leu 
        115                 120                 125             


Ile Cys Ser Leu Met Asn Asp Pro Ala Pro His Asp Pro Arg Leu Val 
    130                 135                 140                 


Tyr Ile Ala Cys Ser Tyr Met Leu Thr His Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Glu Ala Asp Lys Glu Lys Ile Asp Asp Val Leu Asp Ile Arg Arg Val 
                165                 170                 175     


Phe Asn Glu Leu Cys Ser Trp Phe Thr Ser Tyr Gly Ile Glu Val Pro 
            180                 185                 190         


Phe Ser Cys Pro Phe Glu Glu Phe Gly Asn Val Leu Thr Ala Asn Arg 
        195                 200                 205             


Gly Val Gln Asp Arg Thr Arg Ala Phe Tyr Asp Leu Leu Phe Ala Gly 
    210                 215                 220                 


Lys Lys Pro Lys Pro Ser Glu Asp Asp Gln Val Asp Ile Asp Val Leu 
225                 230                 235                 240 


Ile Lys Leu Ile Ser Gly Gly Lys Ile Glu Leu Ser Lys Leu Phe Leu 
                245                 250                 255     


Asn Glu Glu Tyr Ser Glu Leu Glu Asn Asn Lys Ile Gln Leu Ser Asp 
            260                 265                 270         


Ala Gly Phe Asp Glu Lys Glu Pro Glu Leu Arg Ser Glu Leu Gly Asp 
        275                 280                 285             


Asp Phe Gly Leu Val Ala Ala Ala Lys Asn Ile Tyr Asp Trp Ser Leu 
    290                 295                 300                 


Leu Thr Gly Val Leu Gly Glu Cys Lys Ser Ile Ser Glu Ala Lys Val 
305                 310                 315                 320 


Lys Thr Tyr Glu Lys His Ser Glu Asp Leu Ala Ala Leu Arg Arg Val 
                325                 330                 335     


Val Arg Lys Tyr His Pro Asp Lys Tyr Lys Glu Ile Phe Lys Lys Tyr 
            340                 345                 350         


Val Val Gly Asn Tyr Thr Ser Tyr Ser Gly Asn Val Lys Ser Ala Gly 
        355                 360                 365             


Lys Ile Ser Glu Thr Lys Leu Lys Arg Cys Asn Gln Ala Glu Phe Cys 
    370                 375                 380                 


Asp Tyr Leu Lys Ser Lys Leu Lys Asp Val Lys Pro Ala Pro Gly Asp 
385                 390                 395                 400 


Asp Glu Cys Glu Arg Val Leu Lys Glu Ile Ser Lys His Thr Phe Cys 
                405                 410                 415     


Pro Arg Gln Val Thr Thr Asp Asn Arg Val Ile Pro Tyr Gln Leu Tyr 
            420                 425                 430         


Tyr Thr Glu Leu Lys Lys Val Leu Glu Asn Ala Lys Arg Tyr Ile Pro 
        435                 440                 445             


Gln Leu Asn Val Thr Asp Glu Tyr Gly Ser Ala Ala Asp Lys Ile Leu 
    450                 455                 460                 


Ser Leu Met Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Lys 
465                 470                 475                 480 


Lys Asn Asp Ser Ser Asn Ala Trp Ile Val Arg Arg Gly Glu Gly Arg 
                485                 490                 495     


Ile Leu Pro Trp Asn Phe Asp Glu Leu Val Asp Lys Asp Lys Cys Glu 
            500                 505                 510         


Asp Glu Phe Ile Arg Arg Met Thr Cys Lys Cys Thr Tyr Ile Ala Gly 
        515                 520                 525             


Lys Asn Val Leu Pro Lys Asn Ser Leu Leu Tyr Cys Lys Phe Met Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Ile Lys Ile Asn Gly Glu Arg Ile Ser Val 
545                 550                 555                 560 


Gly Asp Lys Gln Lys Ile Tyr Thr Glu Leu Phe Glu Lys Lys Arg Lys 
                565                 570                 575     


Val Thr Val Arg Asp Ile Arg Asn Leu Leu Thr Ser Glu Gly Arg Leu 
            580                 585                 590         


Lys Ala Gly Asp Thr Phe Gly Gly Ile Asp Ile Thr Val Lys Ser Ser 
        595                 600                 605             


Leu Lys Pro Gln His Asp Leu Lys Arg Leu Met Ala Ala Gly Val Ile 
    610                 615                 620                 


Thr Glu Ala Asp Ala Glu Glu Ile Ile Glu Arg Ile Thr Cys Thr Thr 
625                 630                 635                 640 


Asp Val Ser Arg Leu Arg Thr Trp Leu Gly Lys Phe Gly Leu Ser Ala 
                645                 650                 655     


Ala Asp Ala Lys Tyr Val Ser Gly Leu Lys Tyr Ser Asp Phe Gly Arg 
            660                 665                 670         


Leu Ser Arg Glu Phe Leu Thr Glu Val Asn Asp Val Asp Pro Ala Thr 
        675                 680                 685             


Gly Glu Val Ile Asn Pro Asn Ile Ile Thr Met Met Trp Glu Asn Asn 
    690                 695                 700                 


Ile Asn Leu Met Glu Leu Leu Ser Gln Lys Tyr Ser Tyr Arg Ser Gln 
705                 710                 715                 720 


Ile Glu Ser Met Asn Arg Glu Tyr Tyr Ala Ala Asn Pro Leu Thr Leu 
                725                 730                 735     


Asp Glu Arg Met Ser Glu Met Tyr Ile Pro Ser Ala Val Arg Arg Pro 
            740                 745                 750         


Val Lys Arg Thr Ile Glu Met Met Lys Glu Tyr Met His Ile Met Arg 
        755                 760                 765             


Lys Pro Pro Lys Arg Ile Phe Ile Glu Met Ala Arg Glu Leu Thr Asp 
    770                 775                 780                 


Leu Asp Asn Lys Gly Lys Arg Thr Ser Ser Arg Arg Asn Thr Leu Leu 
785                 790                 795                 800 


Glu Phe Phe Lys Lys Cys Pro Gln Asp Thr Thr Glu Leu Arg Ala Gln 
                805                 810                 815     


Leu Glu Lys Lys Ser Asp Asp Glu Leu Arg Ser Glu Lys Leu Tyr Leu 
            820                 825                 830         


Tyr Phe Ala Gln Leu Gly Lys Cys Met Tyr Ser Gly Glu Ser Ile Asp 
        835                 840                 845             


Ile Glu Arg Leu Gly Ser Lys Leu Tyr Asp Val Asp His Ile Phe Pro 
    850                 855                 860                 


Gln Ala Arg Val Lys Asp Asp Ser Ile Glu Asn Lys Val Leu Val Leu 
865                 870                 875                 880 


Ser Asp Leu Asn Gly Ala Lys Ser Asp Arg Tyr Pro Ile Asn Ala Asp 
                885                 890                 895     


Ile Arg Asn Lys Met His Ser Phe Trp Asp Lys Leu His Ser Ile Gly 
            900                 905                 910         


Leu Ile Gly Glu Lys Lys Tyr Glu Arg Leu Thr Arg Ser Thr Gly Phe 
        915                 920                 925             


Thr Asp Asp Glu Leu Ala Gly Phe Ile Ser Arg Gln Leu Val Glu Thr 
    930                 935                 940                 


Arg Gln Ser Thr Lys Ala Val Ala Ala Leu Leu Ala Glu Glu Phe Pro 
945                 950                 955                 960 


Glu Ser Glu Ile Val Tyr Val Lys Ala Gly Leu Ala Ser Gln Phe Arg 
                965                 970                 975     


His Glu Phe Gly Leu Leu Lys Cys Arg Glu Ile Asn Asp Leu His His 
            980                 985                 990         


Ala Lys Asp Ala Tyr Leu Asn Ile  Val Met Gly Asn Ile  Tyr His Val 
        995                 1000                 1005             


Lys Phe  Thr Lys Asp Pro Met  Asn Phe Val Lys Glu  Tyr Arg Arg 
    1010                 1015                 1020             


Lys Glu  Gln Val Phe Asp Leu  Lys Ile Gln Ser Met  Leu Thr His 
    1025                 1030                 1035             


Asp Ala  Ile Arg Asn Gly Glu  Thr Ala Trp Ile Gly  Asp Gly Thr 
    1040                 1045                 1050             


Trp Leu  Glu Thr Val Lys Lys  Gln Met Glu Lys Asn  Asn Ile Arg 
    1055                 1060                 1065             


Tyr Val  Arg Tyr Ser Phe Arg  Arg Lys Gly Ala Leu  Phe Asn Leu 
    1070                 1075                 1080             


Met Pro  Glu Arg Lys Asn Pro  Ser Leu Leu Pro Arg  Lys Asn Gly 
    1085                 1090                 1095             


Lys Asn  Met Val Leu Pro Ala  Glu Lys Tyr Gly Gly  Tyr Asn Asn 
    1100                 1105                 1110             


Thr Ala  Ala Ser Phe Phe Ser  Leu Val Lys Tyr His  Ser Ala Lys 
    1115                 1120                 1125             


Gly Ser  Gly Val Val Ile Ile  Pro Val Glu Leu Ile  His Ala His 
    1130                 1135                 1140             


Arg Tyr  Glu His Asp Ser Ser  Phe Ala Glu Glu Tyr  Ala Leu Arg 
    1145                 1150                 1155             


Val Leu  Ala Asp Ile Val Ser  Leu Lys Lys Gly Asp  Val Leu Gln 
    1160                 1165                 1170             


Ser Val  Glu Phe Pro Leu Gly  Ser Arg Ile Leu Lys  Val Asn Thr 
    1175                 1180                 1185             


Leu Leu  Asp Ile Asp Gly Phe  Arg Ala Asn Ile Thr  Gly Lys Asp 
    1190                 1195                 1200             


Ser Gly  Gly Lys Tyr Leu Gly  Ile Ser Ser Ala Ser  Ser Leu Ile 
    1205                 1210                 1215             


Ile Ser  Gln Val Asp Thr Thr  Tyr Ile Lys Arg Val  Ile Ser Ile 
    1220                 1225                 1230             


Asn Glu  Arg Ile Ser Ala Cys  Glu Lys Asn Lys Gln  Ser Tyr Thr 
    1235                 1240                 1245             


Ile Arg  Pro Glu Phe Asp Val  Ile Thr Ser Glu Arg  Asn Ile Glu 
    1250                 1255                 1260             


Ile Tyr  Asn Met Leu Cys Glu  Lys Ile Ser Ser Arg  Pro Phe Cys 
    1265                 1270                 1275             


Ile Lys  Phe Ala Asn Thr Ser  Asp Thr Phe Val Ser  Gly Arg Glu 
    1280                 1285                 1290             


Lys Phe  Ile Ala Leu Ser Leu  Asn Glu Gln Val Lys  Thr Leu Thr 
    1295                 1300                 1305             


Asn Met  Leu Ser Val Phe Lys  Thr Gly Arg Ala Ser  Gly Cys Asp 
    1310                 1315                 1320             


Leu Thr  Asn Ile Gly Gly Ser  Lys Asn Thr Ala Ile  Val Lys Leu 
    1325                 1330                 1335             


Asn Ala  Val Leu Ser Asn Leu  Lys Asp Phe Lys Ser  Val Arg Ile 
    1340                 1345                 1350             


Ile Asp  Gln Ser Pro Thr Gly  Leu Tyr Glu Lys Val  Ser Pro Asn 
    1355                 1360                 1365             


Leu Leu  Ser Leu 
    1370         


<210>  1045
<211>  1372
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1045

Met Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Ile Pro Ala Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Ala Lys Ala Asp Asn 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys 
            100                 105                 110         


Asp Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala 
        115                 120                 125             


Tyr His Gln Lys Phe Ser Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala 
    130                 135                 140                 


Asp Ser Pro Glu Lys Ser Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Lys 
                165                 170                 175     


Ala Glu Asn Thr Asp Val Gln Lys Leu Phe Thr Asp Phe Val Glu Val 
            180                 185                 190         


Tyr Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Val Thr Val Asp 
        195                 200                 205             


Ala Ser Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Leu Ile Glu Ser Tyr Pro Lys Glu Lys Lys Asn Thr Leu Phe Gly 
225                 230                 235                 240 


Asn Leu Ile Ala Leu Ser Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Val Leu Glu Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Asp Asp Ser Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Lys Arg Tyr Ala Glu His His Glu Asp Leu Glu Lys Leu 
                325                 330                 335     


Lys Ala Phe Ile Lys Thr Asn Lys Pro Asn Leu Tyr His Asp Ile Phe 
            340                 345                 350         


Lys Asp Glu Thr Gln Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val 
        355                 360                 365             


Lys Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Thr Ile Leu Ser Lys Thr 
    370                 375                 380                 


Asp Gly Ser Glu Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asn Ser Arg Phe Ala 
    450                 455                 460                 


Trp Ala Glu Tyr His Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asn 
465                 470                 475                 480 


Lys Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile 
        515                 520                 525             


Lys Tyr Val Asn Glu Gln Gly Lys Glu Ala Phe Phe Asp Ser Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 


Lys Glu Lys Leu Leu Asn Tyr Leu Asp Lys Glu Phe Pro Glu Tyr Arg 
                565                 570                 575     


Ile Gln Asp Leu Ile Gly Leu Asp Lys Glu Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys Ser Phe 
        595                 600                 605             


Leu Asp Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Gln Gln Arg Leu His Lys 
625                 630                 635                 640 


Tyr Ser Asp Ile Phe Ser Thr Gln Gln Leu Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asn Lys Glu Asn Asn Lys Thr Ile Leu Glu Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Tyr Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu 
    690                 695                 700                 


Pro Phe Lys Gln Ile Ile Lys Asp Ala Gln Ile Ile Gly Asn Ile Asp 
705                 710                 715                 720 


Asp Ile Ala Ala Val Val His Glu Leu Pro Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly His Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys 
    770                 775                 780                 


Leu Gln Asp Ser Leu Lys Glu Leu Gly Ser Asn Ile Leu Asn Lys Lys 
785                 790                 795                 800 


Lys Pro Ser Tyr Val Glu Asp Lys Val Glu Asn Val His Leu Gln Asp 
                805                 810                 815     


Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Ile Tyr Thr 
            820                 825                 830         


Gly Glu Glu Leu Asp Ile Asp Arg Leu Ser Asp Tyr Asp Ile Asp His 
        835                 840                 845             


Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val 
    850                 855                 860                 


Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Asn 
865                 870                 875                 880 


Leu Asp Ile Val Arg Ala Arg Lys Ala Asp Trp Val Arg Leu Tyr Lys 
                885                 890                 895     


Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys Ser Glu 
            900                 905                 910         


Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala Gly Phe Ile Lys Arg Gln 
        915                 920                 925             


Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp 
    930                 935                 940                 


Ala Arg Phe Asn Thr Glu His Asp Glu Asp Asp Lys Val Ile Arg Asn 
945                 950                 955                 960 


Val Lys Val Ile Thr Leu Lys Ser Asn Leu Ile Ser Gln Phe Arg Lys 
                965                 970                 975     


Asp Phe Lys Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala 
            980                 985                 990         


His Asp Ala Tyr Leu Asn Ala Val  Val Gly Thr Ala Leu  Leu Arg Lys 
        995                 1000                 1005             


Tyr Pro  Lys Leu Ala Pro Glu  Phe Val Tyr Gly Glu  Tyr Lys Lys 
    1010                 1015                 1020             


Tyr Asp  Val Arg Lys Leu Ile  Thr Ser Ser Ser Asp  Lys Ala Thr 
    1025                 1030                 1035             


Ala Lys  Tyr Phe Phe Tyr Ser  Asn Leu Met Asn Phe  Phe Lys Arg 
    1040                 1045                 1050             


Val Val  Arg Tyr Ser Asn Gly  Arg Val Ile Val Arg  Pro Val Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Val Trp Asn  Lys Glu Thr 
    1070                 1075                 1080             


Asp Phe  Arg Thr Ile Cys Lys  Val Leu Ser Tyr Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Val Glu Ser  Gln Thr Gly Gly Phe  Ser Lys Glu 
    1100                 1105                 1110             


Ser Ile  Leu Pro Lys Gly Asp  Ser Asp Lys Leu Ile  Pro Arg Lys 
    1115                 1120                 1125             


Thr Lys  Lys Val Tyr Trp Asp  Thr Lys Lys Tyr Gly  Gly Phe Asp 
    1130                 1135                 1140             


Ser Pro  Ile Ile Ala Tyr Ser  Val Phe Val Val Ala  Asp Val Glu 
    1145                 1150                 1155             


Lys Gly  Lys Ala Lys Lys Leu  Lys Lys Val Lys Glu  Leu Val Gly 
    1160                 1165                 1170             


Ile Ser  Ile Met Glu Arg Pro  Ser Phe Glu Lys Asn  Pro Ile Val 
    1175                 1180                 1185             


Phe Leu  Glu Lys Lys Gly Tyr  Arg Asn Val Gln Glu  Asp Thr Leu 
    1190                 1195                 1200             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Phe Glu  Gly Gly Arg 
    1205                 1210                 1215             


Arg Arg  Leu Leu Ala Ser Ala  Ser Glu Leu Gln Lys  Gly Asn Glu 
    1220                 1225                 1230             


Val Val  Leu Ser Asp Arg Leu  Val Glu Leu Leu Tyr  His Ala His 
    1235                 1240                 1245             


Arg Val  Asn Ser Phe Asn Ser  Thr Glu His Leu Lys  Tyr Val Ser 
    1250                 1255                 1260             


Glu His  Lys Lys Glu Phe Gly  Glu Val Leu Ser Cys  Val Glu Asn 
    1265                 1270                 1275             


Phe Ala  Asn Ser Tyr Val Asp  Val Glu Lys Asn Leu  Asp Lys Ile 
    1280                 1285                 1290             


Arg Ala  Val Ile Asn Lys Ile  Asp Thr Phe Ser Ile  Glu Glu Ile 
    1295                 1300                 1305             


Ser Thr  Ser Phe Val Asn Leu  Leu Thr Leu Thr Ala  Leu Gly Ala 
    1310                 1315                 1320             


Pro Ala  Asp Phe Asn Phe Leu  Gly Lys Lys Ile Pro  Arg Lys Arg 
    1325                 1330                 1335             


Tyr Thr  Ser Thr Lys Glu Cys  Leu Asn Ala Thr Leu  Ile His Gln 
    1340                 1345                 1350             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu 
    1355                 1360                 1365             


Gly Glu  Asp Lys 
    1370         


<210>  1046
<211>  1372
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1046

Met Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Asn Glu Ile Ala Lys Val Asp Glu 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys 
            100                 105                 110         


Thr Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala 
        115                 120                 125             


Tyr His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn 
                165                 170                 175     


Ala Glu Asn Thr Asp Val Gln Lys Ile Phe Ala Asp Phe Val Gly Val 
            180                 185                 190         


Tyr Asn Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp 
        195                 200                 205             


Val Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Leu Ile Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly 
225                 230                 235                 240 


Asn Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu 
                325                 330                 335     


Lys Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe 
            340                 345                 350         


Lys Asp Lys Asn Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val 
        355                 360                 365             


Lys Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile 
    370                 375                 380                 


Lys Ile Asp Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp 
385                 390                 395                 400 


Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln 
                405                 410                 415     


Ile His Leu Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr 
            420                 425                 430         


Tyr Pro Phe Leu Lys Glu Lys Gln Asp Arg Ile Glu Lys Ile Leu Thr 
        435                 440                 445             


Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Arg Lys Asp Ser Arg 
    450                 455                 460                 


Phe Ala Trp Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn 
465                 470                 475                 480 


Phe Asp Lys Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr 
                485                 490                 495     


Arg Met Thr Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro 
            500                 505                 510         


Lys His Ser His Val Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr 
        515                 520                 525             


Lys Ile Lys Tyr Val Asn Glu Gln Gly Lys Glu Ser Phe Phe Asp Ser 
    530                 535                 540                 


Asn Met Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys 
545                 550                 555                 560 


Val Thr Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu 
                565                 570                 575     


Tyr Arg Ile Lys Asp Leu Ile Gly Leu Asp Lys Glu Asn Lys Ser Phe 
            580                 585                 590         


Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys 
        595                 600                 605             


Ala Phe Leu Asp Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile 
    610                 615                 620                 


Lys Thr Leu Thr Leu Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu 
625                 630                 635                 640 


Gln Lys Tyr Ser Asp Ile Phe Thr Ala Asn Gln Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn 
            660                 665                 670         


Gly Ile Arg Asn Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile 
        675                 680                 685             


Asp Asp Gly Ser Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp 
    690                 695                 700                 


Thr Leu Pro Phe Lys Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp 
705                 710                 715                 720 


Val Asp Asp Ile Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Lys Val Met Gly Gly Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu 
    770                 775                 780                 


Lys Lys Leu Gln Asn Ser Leu Lys Glu Leu Gly Ser Asn Ile Leu Asn 
785                 790                 795                 800 


Glu Glu Lys Pro Ser Tyr Ile Glu Asp Lys Val Glu Asn Ser His Leu 
                805                 810                 815     


Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met 
            820                 825                 830         


Tyr Thr Gly Asp Glu Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile 
        835                 840                 845             


Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn 
    850                 855                 860                 


Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val 
865                 870                 875                 880 


Pro Ser Leu Asp Ile Val Arg Ala Arg Lys Ala Glu Trp Val Arg Leu 
                885                 890                 895     


Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys 
            900                 905                 910         


Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala Gly Phe Ile Lys 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile 
    930                 935                 940                 


Leu Asp Ala Arg Phe Asn Thr Glu His Asp Glu Asn Asp Lys Val Ile 
945                 950                 955                 960 


Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe 
                965                 970                 975     


Arg Lys Asp Phe Glu Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His 
            980                 985                 990         


His Ala His Asp Ala Tyr Leu Asn  Ala Val Val Gly Thr  Ala Leu Leu 
        995                 1000                 1005             


Lys Lys  Tyr Pro Lys Leu Ala  Ser Glu Phe Val Tyr  Gly Glu Tyr 
    1010                 1015                 1020             


Lys Lys  Tyr Asp Ile Arg Lys  Phe Ile Thr Asn Ser  Ser Asp Lys 
    1025                 1030                 1035             


Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn Leu Met  Asn Phe Phe 
    1040                 1045                 1050             


Lys Thr  Lys Val Lys Tyr Ala  Asp Gly Thr Val Phe  Glu Arg Pro 
    1055                 1060                 1065             


Ile Ile  Glu Thr Asn Ala Asp  Gly Glu Ile Ala Trp  Asn Lys Gln 
    1070                 1075                 1080             


Ile Asp  Phe Glu Lys Val Arg  Lys Val Leu Ser Tyr  Pro Gln Val 
    1085                 1090                 1095             


Asn Ile  Val Lys Lys Val Glu  Thr Gln Thr Gly Gly  Phe Ser Lys 
    1100                 1105                 1110             


Glu Ser  Ile Leu Pro Lys Gly  Asp Ser Asp Lys Leu  Ile Pro Arg 
    1115                 1120                 1125             


Lys Thr  Lys Lys Val Tyr Trp  Asp Thr Lys Lys Tyr  Gly Gly Phe 
    1130                 1135                 1140             


Asp Ser  Pro Thr Val Ala Tyr  Ser Val Phe Val Val  Ala Asp Val 
    1145                 1150                 1155             


Glu Lys  Gly Lys Ala Lys Lys  Leu Lys Thr Val Lys  Glu Leu Val 
    1160                 1165                 1170             


Gly Ile  Ser Ile Met Glu Arg  Ser Phe Phe Glu Glu  Asn Pro Val 
    1175                 1180                 1185             


Glu Phe  Leu Glu Asn Lys Gly  Tyr His Asn Ile Arg  Glu Asp Lys 
    1190                 1195                 1200             


Leu Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe Glu Phe  Glu Gly Gly 
    1205                 1210                 1215             


Arg Arg  Arg Leu Leu Ala Ser  Ala Ser Glu Leu Gln  Lys Gly Asn 
    1220                 1225                 1230             


Glu Met  Val Leu Pro Gly Tyr  Leu Val Glu Leu Leu  Tyr His Ala 
    1235                 1240                 1245             


His Arg  Ala Asp Asn Phe Asn  Ser Thr Glu Tyr Leu  Asn Tyr Val 
    1250                 1255                 1260             


Ser Glu  His Lys Lys Glu Phe  Glu Lys Val Leu Ser  Cys Val Glu 
    1265                 1270                 1275             


Asp Phe  Ala Asn Leu Tyr Val  Asp Val Glu Lys Asn  Leu Ser Lys 
    1280                 1285                 1290             


Ile Arg  Ala Val Ala Asp Ser  Met Asp Asn Phe Ser  Ile Glu Glu 
    1295                 1300                 1305             


Ile Ser  Asn Ser Phe Ile Asn  Leu Leu Thr Leu Thr  Ala Leu Gly 
    1310                 1315                 1320             


Ala Pro  Ala Asp Phe Asn Phe  Leu Gly Glu Lys Ile  Pro Arg Lys 
    1325                 1330                 1335             


Arg Tyr  Thr Ser Thr Lys Glu  Cys Leu Asn Ala Thr  Leu Ile His 
    1340                 1345                 1350             


Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Lys 
    1355                 1360                 1365             


Leu Gly  Glu Glu 
    1370         


<210>  1047
<211>  1372
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1047

Met Asn Ser Asp Lys Arg Glu Asp Tyr Tyr Val Gly Leu Asp Met Gly 
1               5                   10                  15      


Thr Gly Ser Leu Gly Gly Ala Val Thr Asp Thr Gln Tyr His Leu Leu 
            20                  25                  30          


Lys Val Lys Gly Lys Asp Phe Trp Phe Val Arg Glu Tyr Glu Thr Ala 
        35                  40                  45              


Lys Ser Gln Leu Asp Arg Arg Thr His Arg Ile Ala Lys Arg Arg Leu 
    50                  55                  60                  


Gln Arg His Lys Ala Arg Ile Gly Leu Ile Arg Ser Tyr Phe Ala Glu 
65                  70                  75                  80  


Asp Ile Leu Lys His Asp Pro Leu Phe Tyr Ile Arg Gln Asp Asn Ser 
                85                  90                  95      


Lys Tyr Tyr His Glu Asp Lys Asp Val Arg Leu Thr Thr Lys Asp Ser 
            100                 105                 110         


Ile Phe Ala Asp Pro Gly Tyr Gly Asp Lys Glu Tyr Leu Asp Asp Lys 
        115                 120                 125             


Glu Tyr Pro Thr Ile Phe His Leu Arg Gln Ala Leu Leu Arg Asp Gln 
    130                 135                 140                 


Ile Lys Asp Glu Glu Arg Tyr Ser Arg Phe Leu Tyr Leu Ala Ile Ile 
145                 150                 155                 160 


Asn Met Phe Glu His Arg Gly His Phe Leu Leu Asn Thr Glu Ser Ser 
                165                 170                 175     


Asp Leu Asn Ala Asp Met Ile Lys Asp Val Gly Glu Leu Val Met Asn 
            180                 185                 190         


Ala Ile Cys Gly Val Ser Glu Tyr Gly Ser Val Thr Tyr Lys Glu Ile 
        195                 200                 205             


Ile Glu Ile Leu Glu Asp Lys Asn Ile Ser Arg Thr Ala Lys Lys Asp 
    210                 215                 220                 


Gln Ile Ala Ser Lys Ile Asp Ile Lys Lys Ser Glu Lys Ala Gln Met 
225                 230                 235                 240 


Glu Arg Ile Lys Cys Leu Cys Gly Met Glu Ala Asp Ala Arg Val Met 
                245                 250                 255     


Phe Glu Ile Glu Ala Asp Glu Lys Ile Lys Ile Ala Phe Gln Gln Ala 
            260                 265                 270         


Ala Phe Glu Asp Glu Lys Glu Glu Ile Glu Ala Val Ile Gly Glu Asp 
        275                 280                 285             


Arg Tyr Leu Ile Ile Glu Asn Met Lys Gln Leu Tyr Asp Tyr Ser Gln 
    290                 295                 300                 


Leu Gln Ser Val Leu Asn Gly Phe Asp Tyr Leu Ser Asp Ala Arg Val 
305                 310                 315                 320 


Gln Met Tyr Glu Lys His Lys Glu Asp Leu His Leu Leu Lys Lys Val 
                325                 330                 335     


Tyr Arg Lys Asn Leu Asp Arg Glu Ile Tyr Asp Arg Met Phe Arg Ser 
            340                 345                 350         


Gly Glu Glu Gly Ser Tyr Cys Ala Tyr Ser Asn Ser Thr Asn Ser Val 
        355                 360                 365             


Asp Ser Leu Ala Glu Ala Lys Lys Tyr Arg Arg Asn Met Lys Lys Arg 
    370                 375                 380                 


Ser Gln Lys Asp Leu Tyr Asp Arg Ile Lys Lys Asp Leu Lys Glu Val 
385                 390                 395                 400 


His Asp Ala Asp Ala Asp Ala Ile Arg Lys Glu Met Glu Leu Glu Arg 
                405                 410                 415     


Phe Leu Pro Lys Gln Ile Thr Gly Ala Asn Gly Ile Ile Pro Asn Gln 
            420                 425                 430         


Val His Arg Lys Glu Leu Lys Lys Ile Leu Glu Asn Ala Glu His His 
        435                 440                 445             


Leu Asp Phe Leu Lys Glu Lys Asp Glu Ser Gly Tyr Thr Val Ser Glu 
    450                 455                 460                 


Arg Ile Leu Met Leu Phe Ser Ser Asn Ile Pro Tyr Tyr Val Gly Pro 
465                 470                 475                 480 


Val Gly Ser Gly Ser Lys Thr Gly Trp Ala Lys Ile Gln Glu Ser Gly 
                485                 490                 495     


Gln Val Leu Pro Trp Asn Met Glu Gln Lys Ile Asp Ile Glu Gln Thr 
            500                 505                 510         


Ser Glu Arg Phe Ile Thr Asn Leu Ile Arg Glu Cys Thr Tyr Leu Ser 
        515                 520                 525             


Gly Glu Lys Val Leu Pro Lys Gln Ser Leu Leu Tyr Glu Ser Tyr Cys 
    530                 535                 540                 


Val Leu Asn Glu Ile Asn Asn Leu Arg Ile His Gly Lys Arg Ile Val 
545                 550                 555                 560 


Pro Glu Leu Lys Gln Asp Ile Tyr Lys Glu Val Phe Gln Gly Phe Arg 
                565                 570                 575     


Leu Gly Lys Lys Val Thr Lys Lys Asn Ile Cys Thr Tyr Leu Ile Asn 
            580                 585                 590         


Arg Gly Leu Ile Thr Glu Glu Ser Glu Val Ser Gly Ile Asp Lys Glu 
        595                 600                 605             


Ile Asn Ala Tyr Leu Pro Ser Tyr Gly Lys Met Tyr Gly Val Phe Gly 
    610                 615                 620                 


Glu Arg Leu Arg Glu Glu Ala Val Gln Glu Ile Ala Glu Lys Ile Ile 
625                 630                 635                 640 


Tyr Tyr Gly Thr Ile Tyr Gly Asp Ala Lys Ser Met Phe Arg Lys Lys 
                645                 650                 655     


Leu Asn Gln Tyr Val Ser Asp Gly Trp Met Thr Glu Tyr Gln Ala Lys 
            660                 665                 670         


Arg Ile Ser Gly Tyr Lys Phe Lys Asp Trp Ala Arg Leu Ser Arg Ser 
        675                 680                 685             


Met Leu Cys Leu Ser Gly Cys Asp Lys Ser Thr Gly Glu Lys Ile Ser 
    690                 695                 700                 


Leu Ile Arg Ala Met Trp Glu Tyr Asn Leu Asn Phe Met Glu Leu Ile 
705                 710                 715                 720 


Tyr Arg Glu Asp Phe Thr Phe Gln Lys Glu Leu Glu Glu Arg Arg Lys 
                725                 730                 735     


Ala Leu Gly Lys Thr Leu Gln Glu Phe Gln Phe Glu Asp Leu Asp Glu 
            740                 745                 750         


Tyr Tyr Tyr Ser Ala Pro Val Lys Arg Met Ile Trp Gln Ala Val Gln 
        755                 760                 765             


Ala Leu Lys Glu Ile Thr Gln Ile Met Gly His Asn Pro Lys Arg Ile 
    770                 775                 780                 


Phe Ile Glu Met Thr Arg Thr Glu Glu Glu Lys Gly Glu Gln Gly Arg 
785                 790                 795                 800 


Lys Asn Ser Arg Glu Lys Arg Leu Leu Lys Met Tyr Glu Ser Ile Arg 
                805                 810                 815     


Asp Glu Arg Asn Trp Ala Ala Glu Ile Lys Asn Ala Asn Ala Ser Gly 
            820                 825                 830         


Lys Leu Asn Ser Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Met Gly Arg 
        835                 840                 845             


Asp Val Tyr Thr Gly Glu Pro Ile Asp Ile Glu Asp Leu Phe Asp Asp 
    850                 855                 860                 


Ser Arg Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Leu Thr Asn Asp 
865                 870                 875                 880 


Asn Asn Ile Glu Asn Asn Leu Val Leu Val Ser Lys Thr Ile Asn Gln 
                885                 890                 895     


Asn Glu Lys Lys Asn Asp Tyr Pro Val Pro Glu Lys Ile Arg Arg Asn 
            900                 905                 910         


Lys Lys Val Arg Asn Leu Trp His Thr Leu His Lys Met Gly Phe Met 
        915                 920                 925             


Asn Asp Glu Lys Tyr Asn Arg Leu Thr Ser Thr Glu Arg Leu Ser Glu 
    930                 935                 940                 


Asp Gln Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Ala Gln 
945                 950                 955                 960 


Gly Ala Lys Gly Ile Ala Glu Leu Met Lys Ala Met Leu Pro Gly Thr 
                965                 970                 975     


Asp Ile Val Tyr Ala Lys Ala Arg Asn Val Ser Asp Phe Arg Lys Glu 
            980                 985                 990         


Tyr Lys Met Leu Lys Ser Arg Leu  Val Asn Asp Tyr His  His Ala Gln 
        995                 1000                 1005             


Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn Val Tyr  Phe Met Lys 
    1010                 1015                 1020             


Phe Thr  Arg Asn Pro Met Asn  Phe Val Lys Thr Glu  Ala Arg Arg 
    1025                 1030                 1035             


Asp Gly  Arg Asn Tyr Asp Tyr  Asn Leu Tyr Lys Met  Tyr Ser Lys 
    1040                 1045                 1050             


Asp Ile  Ile Arg Asn Gly Glu  Lys Ala Trp Ile Ala  Thr Ser Glu 
    1055                 1060                 1065             


Gln Gly  Pro Gly Thr Ile Arg  Leu Val Lys Glu Thr  Met Gly Arg 
    1070                 1075                 1080             


Asn Thr  Pro Ile Ile Thr Arg  Gln Ala Phe Glu Gln  Arg Gly Glu 
    1085                 1090                 1095             


Leu Phe  Asn Leu Gln Pro Val  Gly Lys Tyr Ser Ala  Lys Lys Asp 
    1100                 1105                 1110             


Asn Tyr  Val Pro Leu Lys Ile  Asn Asp Glu Lys Met  Gln Asp Val 
    1115                 1120                 1125             


Ser Lys  Tyr Gly Gly Tyr Thr  Ser Leu Asn Pro Ser  Tyr Phe Ile 
    1130                 1135                 1140             


Phe Ile  Glu His Gly Pro Glu  Lys Lys Arg Lys Lys  Cys Phe Glu 
    1145                 1150                 1155             


Val Ile  His Ser Tyr Tyr Ala  Ala Gln Ile Lys Thr  Glu Lys Asp 
    1160                 1165                 1170             


Leu Ile  Asp Phe Leu Leu Gln  Lys Gly Tyr Lys Asn  Pro Arg Val 
    1175                 1180                 1185             


Ile Asn  Ala Arg Ile Lys Lys  Asn Ala Leu Ile Lys  Tyr Asn Gly 
    1190                 1195                 1200             


Tyr Phe  Leu Tyr Ile Ile Gly  Met Asp Ala Arg Lys  Asn Ile Glu 
    1205                 1210                 1215             


Phe Ser  Asn Ala Thr Ala Met  Cys Leu Lys Asn Lys  Tyr Ile Gln 
    1220                 1225                 1230             


Tyr Val  Cys Lys Leu Glu Lys  Met Asn Lys Ala Ile  Leu Leu Ser 
    1235                 1240                 1245             


Glu Lys  Gln Lys Thr Asn Leu  His Trp Asp Glu Lys  Ile Thr Cys 
    1250                 1255                 1260             


Glu Ser  Asn Leu Glu Leu Tyr  Arg Glu Leu Thr Glu  Lys His Leu 
    1265                 1270                 1275             


His Ser  Ile Tyr Gln Arg His  Pro Arg Ser Ile Gly  Lys Cys Leu 
    1280                 1285                 1290             


Ala Asp  Gly Glu Gly Ala Phe  Lys Leu Leu Asp Ile  Glu Glu Gln 
    1295                 1300                 1305             


Val Lys  Ile Ile Cys Asp Ile  Val Gln Tyr Thr Ser  Phe Gln Arg 
    1310                 1315                 1320             


Gly Val  Phe Ser Leu Lys Val  Leu Gly Gly Pro Lys  Glu Val Gly 
    1325                 1330                 1335             


Arg Ile  Arg Ile Ser Gly Asn  Met Thr Glu Ala Lys  Glu Cys Lys 
    1340                 1345                 1350             


Leu Val  Asn Tyr Ser Ile Thr  Gly Met Tyr Lys Thr  Glu Met Asp 
    1355                 1360                 1365             


Leu Leu  Lys Lys 
    1370         


<210>  1048
<211>  1373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1048

Met Glu Lys Thr Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Val Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Lys Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         


Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             


His Gln Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     


Glu Asn Thr Asp Ile Gln Lys Leu Phe Asn Asp Phe Val Glu Thr Tyr 
            180                 185                 190         


Asp Lys Ile Ala Asp Glu Ser His Leu Ser Glu Val Asp Ala Ser Ser 
        195                 200                 205             


Ile Leu Thr Asn Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile 
    210                 215                 220                 


Lys Gln Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn Leu Val 
225                 230                 235                 240 


Ala Leu Val Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe Lys Leu 
                245                 250                 255     


Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Asp Glu Asp 
            260                 265                 270         


Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp Leu Phe 
        275                 280                 285             


Thr Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly Ile Leu 
    290                 295                 300                 


Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser Met Ile 
305                 310                 315                 320 


Lys Arg Tyr Glu Glu His His Glu Asp Leu Glu Lys Leu Lys Lys Phe 
                325                 330                 335     


Ile Lys Val Asn Asn Phe Asp Lys Tyr His Glu Ile Phe Lys Asp Lys 
            340                 345                 350         


Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys Gln Asp 
        355                 360                 365             


Ile Phe Tyr Lys His Leu Lys Ser Ile Ile Ser Glu Lys Asn Gly Gly 
    370                 375                 380                 


Gln Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg Lys Gln 
385                 390                 395                 400 


Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gln Glu 
                405                 410                 415     


Met Arg Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe Leu Lys 
            420                 425                 430         


Glu Asn Gln Ala Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp Ala Asn 
    450                 455                 460                 


Tyr His Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu Val Val 
465                 470                 475                 480 


Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr Leu Asn 
                485                 490                 495     


Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser His Val 
            500                 505                 510         


Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Ile Lys Tyr Val 
        515                 520                 525             


Asn Glu Gln Gly Glu Ser Phe Phe Phe Asp Ala Asn Met Lys Gln Glu 
    530                 535                 540                 


Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys Ala Lys 
545                 550                 555                 560 


Leu Leu Ser Tyr Leu Asn Asn Glu Phe Glu Glu Phe Arg Ile Asn Asp 
                565                 570                 575     


Leu Ile Gly Leu Asp Lys Asp Ser Lys Ser Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             


Lys Thr Asn Glu Gln Ile Ile Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 


Phe Phe Thr Ser Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Asn Lys Thr Ile Leu Asp Phe Leu Ile Asp Asp Gly His Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Glu Ser Leu Ser Phe Lys 
    690                 695                 700                 


Thr Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 


Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Asp 
            740                 745                 750         


Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Asn Arg Glu Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu Gln Asn 
    770                 775                 780                 


Ser Leu Lys Glu Leu Gly Ser Asn Ile Leu Asn Glu Glu Lys Pro Ser 
785                 790                 795                 800 


Tyr Ile Glu Asp Lys Val Glu Asn Ser His Leu Gln Asn Asp Gln Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr Thr Gly Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Asp Ile 
865                 870                 875                 880 


Val Arg Ala Arg Lys Ala Glu Trp Val Arg Leu Tyr Lys Ser Gly Leu 
                885                 890                 895     


Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Glu Ala Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe 
    930                 935                 940                 


Asn Thr Lys Arg Asp Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val 
945                 950                 955                 960 


Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Lys 
                965                 970                 975     


Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Gly Thr  Ala Leu Leu Lys Lys  Tyr Pro Lys 
        995                 1000                 1005             


Leu Thr  Pro Glu Phe Val Tyr  Gly Glu Tyr Lys Lys  Tyr Asp Val 
    1010                 1015                 1020             


Arg Lys  Leu Ile Ala Lys Ser  Ser Asp Asp Tyr Ser  Glu Met Gly 
    1025                 1030                 1035             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Leu  Met Asn Phe 
    1040                 1045                 1050             


Phe Lys  Thr Glu Val Lys Tyr  Ala Asp Gly Arg Val  Phe Glu Arg 
    1055                 1060                 1065             


Pro Asp  Ile Glu Thr Asn Ala  Asp Gly Glu Val Val  Trp Asn Lys 
    1070                 1075                 1080             


Gln Lys  Asp Phe Asp Ile Val  Arg Lys Val Leu Ser  Tyr Pro Gln 
    1085                 1090                 1095             


Val Asn  Ile Val Lys Lys Val  Glu Glu Gln Thr Val  Gly Gln Asn 
    1100                 1105                 1110             


Gly Gly  Leu Phe Asp Asn Asn  Ile Leu Ala Lys Pro  Glu Lys Asp 
    1115                 1120                 1125             


Asp Lys  Lys Lys Leu Phe Pro  Ile Lys Lys Ser Leu  Ser Thr Thr 
    1130                 1135                 1140             


Ser Tyr  Gly Gly Tyr Ala Arg  Ala Thr Ile Ala Tyr  Ser Ile Leu 
    1145                 1150                 1155             


Ile Val  Ser Glu Phe Gly Lys  Asn Lys Glu Leu Lys  Leu Leu Gly 
    1160                 1165                 1170             


Leu Pro  Leu Leu Asp Lys Val  Lys Tyr Glu Lys Asn  Pro Ile Ala 
    1175                 1180                 1185             


Tyr Leu  Asn Ser Ile Gly Ile  Ile Asn Val Lys Ser  Ile Phe Lys 
    1190                 1195                 1200             


Leu Pro  Lys Tyr Cys Leu Phe  Glu Phe Gln Glu Lys  Asn Thr Asn 
    1205                 1210                 1215             


Tyr Arg  Arg Tyr Met Val Ser  Asn Gln Glu Leu Lys  Arg Ala Asn 
    1220                 1225                 1230             


Gln Leu  Phe Leu Thr Cys Lys  Glu Thr Glu Leu Val  Tyr Lys Tyr 
    1235                 1240                 1245             


Ile Lys  Ser Asp Asn Asp Asn  Thr His Leu Val Gly  Asn Glu Lys 
    1250                 1255                 1260             


Thr Ile  Leu Ser Ile Trp Asn  Lys Leu Val Ile Phe  Met Glu Arg 
    1265                 1270                 1275             


Thr Lys  Leu Ile Asp Lys Lys  Leu Phe Ala Ser Leu  Lys Gln Leu 
    1280                 1285                 1290             


Lys Asp  Glu Val Asp Leu Ile  Asn Ile Leu Asp Met  Thr Ala Lys 
    1295                 1300                 1305             


Ser Leu  Asp Phe Thr Lys Ile  Ser Ala Gly Gln Lys  Lys Ile Thr 
    1310                 1315                 1320             


Ile Ser  Asp Ser Leu Ser Leu  Pro Ile Lys Arg Trp  Gln Phe Ser 
    1325                 1330                 1335             


Ser Asp  Glu Lys Met Leu Lys  Gln Leu Lys Thr Ala  Thr Leu Ile 
    1340                 1345                 1350             


His Gln  Ser Val Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser 
    1355                 1360                 1365             


Lys Leu  Gly Glu Glu 
    1370             


<210>  1049
<211>  1373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1049

Met Glu Lys Thr Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Val Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Lys Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         


Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             


His Gln Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     


Glu Asn Thr Asp Ile Gln Lys Leu Phe Asn Asp Phe Val Glu Thr Tyr 
            180                 185                 190         


Asp Lys Ile Ala Asp Glu Ser His Leu Ser Glu Val Asp Ala Ser Ser 
        195                 200                 205             


Ile Leu Thr Asn Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile 
    210                 215                 220                 


Lys Gln Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn Leu Val 
225                 230                 235                 240 


Ala Leu Val Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe Lys Leu 
                245                 250                 255     


Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Asp Glu Asp 
            260                 265                 270         


Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp Leu Phe 
        275                 280                 285             


Thr Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly Ile Leu 
    290                 295                 300                 


Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser Met Ile 
305                 310                 315                 320 


Lys Arg Tyr Glu Glu His His Glu Asp Leu Glu Lys Leu Lys Lys Phe 
                325                 330                 335     


Ile Lys Val Asn Asn Phe Asp Lys Tyr His Glu Ile Phe Lys Asp Lys 
            340                 345                 350         


Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys Gln Asp 
        355                 360                 365             


Ile Phe Tyr Lys His Leu Lys Ser Ile Ile Ser Glu Lys Asn Gly Gly 
    370                 375                 380                 


Gln Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg Lys Gln 
385                 390                 395                 400 


Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gln Glu 
                405                 410                 415     


Met Arg Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe Leu Lys 
            420                 425                 430         


Glu Asn Gln Ser Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr 
        435                 440                 445             


Tyr Val Gly Pro Leu Ala Arg Lys Asn Ser Arg Phe Ala Trp Ala Lys 
    450                 455                 460                 


Tyr His Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu Val Val 
465                 470                 475                 480 


Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr Leu Asn 
                485                 490                 495     


Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser His Val 
            500                 505                 510         


Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys Tyr Val 
        515                 520                 525             


Asn Glu Gln Gly Lys Glu Ser Phe Phe Asp Ser Asn Met Lys Gln Glu 
    530                 535                 540                 


Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys Glu Lys 
545                 550                 555                 560 


Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg Ile Lys Asp 
                565                 570                 575     


Leu Ile Gly Leu Asp Lys Glu Asn Lys Ser Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             


Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 


Ile Phe Thr Ala Asn Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Pro Phe Lys 
    690                 695                 700                 


Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 


Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Gly 
            740                 745                 750         


Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu Gln Ser 
    770                 775                 780                 


Ser Leu Lys Glu Leu Gly Ser Asn Ile Ile Asn Glu Glu Lys Pro Ser 
785                 790                 795                 800 


Tyr Ile Glu Asp Lys Val Glu Asn Ser His Leu Gln Asn Asp Gln Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr Thr Gly Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Asp Ile 
865                 870                 875                 880 


Val Arg Ala Arg Lys Ala Glu Trp Val Arg Leu Tyr Lys Ser Gly Leu 
                885                 890                 895     


Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Glu Ala Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe 
    930                 935                 940                 


Asn Thr Glu His Asp Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val 
945                 950                 955                 960 


Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Asp Phe Glu 
                965                 970                 975     


Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Gly Thr  Ala Leu Leu Lys Lys  Tyr Pro Lys 
        995                 1000                 1005             


Leu Ala  Pro Glu Phe Val Tyr  Gly Glu Tyr Lys Lys  Tyr Asp Val 
    1010                 1015                 1020             


Arg Lys  Leu Ile Ala Lys Ser  Ser Asp Asp His Ser  Glu Met Gly 
    1025                 1030                 1035             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Leu  Met Asn Phe 
    1040                 1045                 1050             


Phe Lys  Thr Lys Val Lys Tyr  Ala Asp Gly Ser Val  Tyr Glu Arg 
    1055                 1060                 1065             


Pro Ile  Ile Glu Thr Asn Ala  Asp Gly Glu Val Val  Trp Asn Lys 
    1070                 1075                 1080             


Gln Lys  Asp Phe Glu Ile Ile  Arg Lys Val Leu Ser  Tyr Pro Gln 
    1085                 1090                 1095             


Val Asn  Ile Val Lys Lys Val  Glu Glu Gln Thr Val  Gly Gln Asn 
    1100                 1105                 1110             


Gly Gly  Leu Phe Asp Asn Asn  Ile Leu Ala Lys Pro  Glu Lys Asp 
    1115                 1120                 1125             


Asp Lys  Lys Lys Leu Phe Pro  Ile Lys Lys Ser Leu  Ser Thr Thr 
    1130                 1135                 1140             


Ser Tyr  Gly Gly Tyr Ala Arg  Ala Thr Ile Ala Tyr  Ser Ile Leu 
    1145                 1150                 1155             


Ile Val  Ser Glu Phe Gly Lys  Asn Lys Glu Leu Lys  Leu Leu Gly 
    1160                 1165                 1170             


Leu Pro  Leu Leu Asp Lys Val  Lys Tyr Glu Lys Asn  Pro Ile Ala 
    1175                 1180                 1185             


Tyr Leu  Asn Ser Ile Gly Ile  Ile Asn Val Lys Ser  Ile Phe Lys 
    1190                 1195                 1200             


Leu Pro  Lys Tyr Cys Leu Phe  Glu Phe Gln Glu Lys  Asn Thr Asn 
    1205                 1210                 1215             


Tyr Arg  Arg Tyr Met Val Ser  Asn Gln Glu Leu Lys  Arg Ala Asn 
    1220                 1225                 1230             


Gln Leu  Phe Leu Thr Cys Lys  Glu Thr Glu Leu Val  Tyr Lys Tyr 
    1235                 1240                 1245             


Ile Lys  Ser Asp Asn Asp Asn  Thr His Leu Val Gly  Asn Glu Lys 
    1250                 1255                 1260             


Thr Ile  Leu Ser Ile Trp Asn  Lys Leu Val Ile Phe  Met Glu Arg 
    1265                 1270                 1275             


Thr Lys  Leu Ile Asp Lys Lys  Leu Phe Ala Ser Leu  Lys Gln Leu 
    1280                 1285                 1290             


Lys Asp  Glu Val Asn Leu Ile  Asn Ile Leu Asp Ile  Ala Ser Lys 
    1295                 1300                 1305             


Ser Ile  Asp Phe Thr Lys Ile  Ser Ala Gly Gln Lys  Lys Ile Val 
    1310                 1315                 1320             


Leu Asn  Asp Ser Leu Ser Leu  Pro Ile Lys Arg Trp  Gln Phe Lys 
    1325                 1330                 1335             


Ser Asp  Glu Lys Met Leu Lys  Gln Leu Asn Asp Ala  Thr Leu Ile 
    1340                 1345                 1350             


His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser 
    1355                 1360                 1365             


Lys Leu  Gly Glu Glu 
    1370             


<210>  1050
<211>  1373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1050

Met Asn Leu Arg Arg Ala Arg Asp Tyr Ser Ile Gly Leu Asp Leu Gly 
1               5                   10                  15      


Thr Gly Ser Val Gly Trp Ala Val Thr Asp Ala Glu Gly Asn Leu Tyr 
            20                  25                  30          


Ser Phe Lys Gly Arg Pro Ala Met Gly Ser Arg Thr Phe Pro Asn Ala 
        35                  40                  45              


Glu Pro Ala Ser Val Ala Arg Ile Pro Arg Gly Gln Arg Arg Arg Tyr 
    50                  55                  60                  


Glu Arg Arg Arg Gln Arg Ile Lys Leu Leu Gln Gln Phe Phe Met Ser 
65                  70                  75                  80  


Asp Met Glu Arg Val Asp Pro Asp Phe Phe Thr Arg Leu Asn Gln Ser 
                85                  90                  95      


Thr Leu Leu Ala Glu Asp Lys Asp Glu Arg Ile Ala Ser Cys Ala Trp 
            100                 105                 110         


Pro Ile Phe Asn Ser Ser Asp Phe Thr Glu Lys Asp Tyr Tyr Lys Lys 
        115                 120                 125             


Phe Pro Thr Ile Tyr His Leu Arg Ala Phe Leu Cys Glu Ser Asp Glu 
    130                 135                 140                 


Lys Gly Asp Leu Arg Leu Val Tyr Leu Ala Leu His Asn Ile Val Lys 
145                 150                 155                 160 


His Arg Gly Asn Phe Leu Tyr Gln Asp Asn Pro Ala Leu Ser Ala Ser 
                165                 170                 175     


Asp Ala Ser Val Asp Glu Ala Val Arg Gln Leu Val Asp Ala Leu Ala 
            180                 185                 190         


Glu Trp Cys Gln Asn Lys Glu Ile Glu Leu Ser Cys Asp Ala Gly Arg 
        195                 200                 205             


Ile Ile Glu Val Phe Gly Asp Ala Ser Leu Arg Arg Thr Asp Lys Arg 
    210                 215                 220                 


Asp Gln Leu Lys Gly Leu Leu Gly Leu Pro Ala Asp Leu Ala Lys Thr 
225                 230                 235                 240 


Met Gly Lys Glu Leu Ala Ser Ala Val Leu Gly Tyr Ala Ala Asn Phe 
                245                 250                 255     


Ser Lys Ile Phe Leu Ile Ser Asp Ala Glu His Lys Phe Ser Leu Gly 
            260                 265                 270         


Asn Asp Glu Lys Val Glu Glu Phe Glu Thr Ile Cys Pro Asn Glu Cys 
        275                 280                 285             


Thr Ser Leu Phe Ser Ala Leu Arg Thr Ala Tyr Ser Ala Tyr Leu Leu 
    290                 295                 300                 


Ser Gly Ile Leu Arg Asp Ala Asn Gly Lys Thr Leu Ser Phe Cys Lys 
305                 310                 315                 320 


Lys Lys Glu Tyr Asp Arg Tyr His Asp Asp Leu Ala Leu Leu Lys Ala 
                325                 330                 335     


Leu Val Arg Glu Tyr Ala Pro Glu Ser Tyr Gln Ser Phe Phe Gly Gly 
            340                 345                 350         


Glu Arg Leu Val Val Asn Gly Arg Met Thr Lys Arg Tyr Asp Pro Thr 
        355                 360                 365             


Lys Ala Lys Gly Tyr Thr Lys Tyr Asp Leu Val His Ser Ala Gly Ser 
    370                 375                 380                 


Tyr Asp Glu Leu Ala Lys Asp Val Lys Lys Leu Leu Gly Ser Leu Ala 
385                 390                 395                 400 


Ala Asp Asp Ser Arg Tyr Gln Arg Met Met Gln Ala Phe Asp Glu Glu 
                405                 410                 415     


Arg Phe Leu Arg Arg Leu Lys Thr Ser Asp Asn Gly Ser Ile Pro Tyr 
            420                 425                 430         


Gln Leu His Leu Glu Glu Leu Arg Ala Ile Ile Ala Arg Gln Gly Glu 
        435                 440                 445             


Phe Tyr Pro Phe Leu Lys Glu Glu Ser Lys Lys Ile Glu Ser Leu Val 
    450                 455                 460                 


Ser Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Thr Thr Lys Asn Ala 
465                 470                 475                 480 


Ala Val Asp Val His Gly Asn Lys Arg Phe Ala Trp Ser Glu Arg Lys 
                485                 490                 495     


Pro Gly Met Glu Ser Ala Lys Val Arg Pro Trp Asn Trp Asp Asp Val 
            500                 505                 510         


Ile Asp Arg Gly Lys Ser Ala Glu Asn Phe Ile Val Arg Met Thr Gly 
        515                 520                 525             


Thr Cys Thr Tyr Leu Gln Asn Glu Lys Val Leu Pro Lys Cys Ser Leu 
    530                 535                 540                 


Leu Tyr Glu Glu Phe Cys Val Leu Asn Glu Leu Asn Gly Ala His Trp 
545                 550                 555                 560 


Ser Ala Asp Gly Asp His Glu Cys Pro Phe Asp Thr Ser Asp Arg Arg 
                565                 570                 575     


Gly Ile Val Gln Asp Leu Phe Cys Gly Lys Arg Arg Val Ser Tyr Lys 
            580                 585                 590         


Gln Val Glu Asp Trp Leu Arg Gln His Gly Ser Arg Arg Ala Arg Val 
        595                 600                 605             


Ser Gly Gly Gln Gly Ala Ser Gly Phe Glu Ser Lys Met Ser Ser Tyr 
    610                 615                 620                 


Ile Phe Phe Ser Lys Asp Val Leu His Val Asp Arg Ile Asp Pro Ala 
625                 630                 635                 640 


Asp Phe Pro Met Ile Glu Glu Ile Ile Leu Trp Asn Thr Leu Phe Glu 
                645                 650                 655     


Asp Arg Asp Ile Leu Lys Glu Arg Ile Glu Glu Ala Tyr Gly Asp Arg 
            660                 665                 670         


Leu Asn Ala Glu Gln Ile Lys Thr Ile Cys Lys Lys Arg Phe Thr Gly 
        675                 680                 685             


Trp Gly Arg Leu Ser Arg Arg Leu Leu Thr Gly Ile Lys Ala Gln Thr 
    690                 695                 700                 


Asp Gln Gly Asp Leu Ser Ile Met Asp Ile Leu Arg Glu Gly Asp Pro 
705                 710                 715                 720 


Asn Asn Asp His Arg Gly Lys Ala Met Val Phe Met Gly Ile Leu His 
                725                 730                 735     


Ser Glu Asp Leu Gly Phe Gln Lys Lys Ile Asp Gln Ile Asn Gln Glu 
            740                 745                 750         


Lys Val Ser Ala Gly Lys Val Ser Leu Glu Asp Leu Pro Gly Ser Pro 
        755                 760                 765             


Ala Leu Arg Arg Ser Ile Asn Gln Ala Met Arg Val Val Asp Glu Ile 
    770                 775                 780                 


Val Ser Ile Ala Lys Cys Pro Pro Ser His Ile Tyr Leu Glu Val Thr 
785                 790                 795                 800 


Arg Asp Glu Asp Gly Ala Arg Lys Gly Arg Arg Ser Thr Arg Arg Tyr 
                805                 810                 815     


Asp Ala Ile Lys Ala Ala Leu Glu Lys Phe Lys Glu Glu Ser Pro Thr 
            820                 825                 830         


Trp Phe Asp Gln Asp Val Phe Gly Glu Phe Arg Arg Ser Arg Ala Glu 
        835                 840                 845             


Asp Leu Asp Glu Arg Leu Ser Leu Tyr Phe Met Gln Gly Gly Lys Ser 
    850                 855                 860                 


Leu Tyr Ser Ala Thr Pro Leu Lys Leu Glu Leu Leu His Thr Asn Gln 
865                 870                 875                 880 


Tyr Gln Val Asp His Ile Ile Pro Gln Ser Tyr Ile Lys Asp Asp Ser 
                885                 890                 895     


Phe Glu Asn Lys Ala Leu Val Leu Ala Gly Glu Asn Gln Arg Lys Ser 
            900                 905                 910         


Asp Ala Leu Leu Ile Asp Ser Ser Ile Arg Thr Ala Met Ala Pro Tyr 
        915                 920                 925             


Trp Arg Ala Leu His Gln Ala Gly Leu Met Gly Asp Lys Lys Phe Asn 
    930                 935                 940                 


Ser Leu Met Cys Ser Ser Ile Ser Asp Lys Arg Val Arg Gly Phe Ile 
945                 950                 955                 960 


Ala Arg Gln Leu Val Glu Thr Ser Gln Ile Val Lys Leu Ala Gly Met 
                965                 970                 975     


Met Leu Gln Ser Asn Tyr Pro Glu Ala Asp Val Gln Pro Val Lys Ala 
            980                 985                 990         


Ser Leu Gly His Asp Leu Arg Val  Gln Ala Gly Phe Ile  Lys Cys Arg 
        995                 1000                 1005             


Glu Val  Asn Asp Phe His His  Ala His Asp Ala Leu  Ile Ala Cys 
    1010                 1015                 1020             


Glu Val  Gly Arg Tyr Ile Asn  Gln Cys His Pro Gly  Ile Tyr Asp 
    1025                 1030                 1035             


Asn Pro  Ile Gly Tyr Ala Arg  Ala Val Arg Glu Leu  Val Arg Lys 
    1040                 1045                 1050             


Gln Asn  Glu Glu Met Arg Lys  Tyr Gly Arg Leu Pro  Gly Ser Ala 
    1055                 1060                 1065             


Gly Phe  Ile Val Gly Gly Phe  Met Arg Thr His Val  Asp Val Asp 
    1070                 1075                 1080             


Thr Gly  Glu Val Val Trp Asp  Ala Glu Glu Glu Leu  Ser Arg Ile 
    1085                 1090                 1095             


Arg Ala  Tyr Leu Asp Tyr Ser  Asn Val Phe Val Thr  Arg Met Ser 
    1100                 1105                 1110             


Glu Glu  Thr Ser Gly Ala Leu  Trp Asn Ala Thr Ile  Tyr Ser Pro 
    1115                 1120                 1125             


His Tyr  Gly Lys Thr Ala Pro  Thr Leu His Ile Lys  Gln Gly Leu 
    1130                 1135                 1140             


Asp Val  Gln Arg Tyr Gly Gly  Tyr Ser Ser Ala Thr  Phe Ala Tyr 
    1145                 1150                 1155             


Phe Thr  Leu Tyr Val Ala Glu  Lys Gly Lys Lys Arg  Lys Ala Glu 
    1160                 1165                 1170             


Leu Leu  Gly Ile Pro Ile Ser  Val Ala Arg Lys Ile  Glu Gly Glu 
    1175                 1180                 1185             


Pro Ser  Lys Leu Asp Ala Phe  Val Ala Gln Asp Ala  Glu Ala Lys 
    1190                 1195                 1200             


Lys Leu  Lys His Pro Leu Ile  Ile Arg Ala Arg Ile  Leu Lys Tyr 
    1205                 1210                 1215             


Ala Arg  Phe Glu Met Glu Gly  Asn Gln Phe Tyr Phe  Ala Ala Gln 
    1220                 1225                 1230             


Asp Ala  Val Tyr Ser Ala Arg  Gln Leu Val Leu Lys  Gly Asp Leu 
    1235                 1240                 1245             


Tyr Ser  Val Ala Ala Lys Ala  Cys Asp Lys His Leu  Asn Asp Ser 
    1250                 1255                 1260             


Cys Ile  Ser Asn Glu Asp Leu  Ile Arg Leu Tyr Asp  Glu Leu Val 
    1265                 1270                 1275             


His Lys  Gly Asp Leu Leu Cys  Asn Lys Phe Gly Asn  Val Thr Asp 
    1280                 1285                 1290             


Val Leu  Arg Ala Gly Arg Glu  Arg Tyr Ala Gly Leu  Ser Ile Ser 
    1295                 1300                 1305             


Glu Lys  Arg Gln Leu Val Cys  Asn Val Leu Ala Tyr  Tyr Arg Ser 
    1310                 1315                 1320             


Ala Thr  Ala Arg Val Asp Ile  Ser Leu Val Gly Gly  Pro Lys His 
    1325                 1330                 1335             


Ala Gly  Asn Leu Gly Ile Ala  Phe Val Leu Asn Asn  Pro Glu Ala 
    1340                 1345                 1350             


Ile Val  Phe Val Asp Gln Ser  Val Thr Gly Met Phe  Glu Lys Arg 
    1355                 1360                 1365             


Thr Arg  Val Glu Leu 
    1370             


<210>  1051
<211>  1373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1051

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Asp Asn Glu Arg Glu Lys Ser Ser Ile Ile 
            20                  25                  30          


Lys Ile Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Gln Gln Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu Gln Arg Phe Lys Leu Arg Lys Glu Asn 
65                  70                  75                  80  


Leu Ile Glu Ile Leu Lys Lys Gln Lys Phe Ile Asn Asp Glu Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Lys Ser Thr Phe Glu Thr Tyr Asn Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Thr Glu Glu Ile Thr Leu Glu Gln Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Ser Gln Asp Glu Gly Gln Leu Ile Asp Gly Met Glu Ile Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Asn Asp Asn Leu Thr Pro Gly Gln Phe Val Phe Gln Ile 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Tyr Ile Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asp Lys Ile Trp Ser Phe Gln Lys Gln Phe Asn Pro 
        195                 200                 205             


Asp Ile Leu Thr Glu Glu Phe Asn Lys Gln Ile Ile Gly Lys Gly Lys 
    210                 215                 220                 


Val Asn Thr Thr Lys Ile Phe Leu Ala Lys Tyr Gly Ile Tyr Thr Ile 
225                 230                 235                 240 


Asp Asn Lys Gly Ala Asp Lys Lys Ser Gln Ala Tyr Asn Trp Arg Val 
                245                 250                 255     


Glu Ala Leu Thr Asn Gln Leu Thr Lys Glu Glu Leu Ala Phe Val Ile 
            260                 265                 270         


Ser Asp Leu Asn Gly Ile Ile Ser Asn Ser Ser Gly Tyr Leu Gly Ser 
        275                 280                 285             


Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr Val Gly 
    290                 295                 300                 


Gln Tyr Leu Ile Ala Gln Leu Lys Glu Asn Pro His Thr Gln Leu Lys 
305                 310                 315                 320 


Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Asn Thr Ile 
                325                 330                 335     


Trp Glu Thr Gln Ala Lys Phe His Lys Glu Leu Thr Asn Glu Leu Lys 
            340                 345                 350         


Glu Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Lys Leu Lys Ser 
        355                 360                 365             


Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Lys Gln Ile Glu 
    370                 375                 380                 


Ile Asn Ile Asp Gly Lys Lys Lys Leu Lys Thr Val Gly Ser Lys Val 
385                 390                 395                 400 


Cys Pro Lys Ser Ser Pro Leu Phe Gln Val Phe Lys Ile Trp Gln Ile 
                405                 410                 415     


Leu Asn Asn Ile Gln Val Ile Glu Lys Ser Thr Gly Lys Ser Lys Phe 
            420                 425                 430         


Leu Glu Gln Asp Glu Lys Glu Ile Leu Phe Lys Glu Leu Asn Phe Lys 
        435                 440                 445             


Asp Lys Ile Ser Lys Thr Asp Ala Leu Lys Ile Leu Tyr Lys Asn Tyr 
    450                 455                 460                 


Lys Glu Leu Asp Phe Asn Phe Lys Glu Asn Ile Glu Gly Asn Arg Thr 
465                 470                 475                 480 


Gln Ala Thr Phe Leu Lys Ala Phe Gln Ser Ile Ile Glu Leu Ser Gly 
                485                 490                 495     


His Glu Val Ser Asp Phe Ser Lys Ile Lys Thr Asp Glu Ile Leu Glu 
            500                 505                 510         


Ile Val Thr Pro Ile Phe Glu Gly Phe Gly Phe Asn Thr Asn Leu Leu 
        515                 520                 525             


Gln Phe Asn Ser Asn Leu Lys Gly Lys Asp Phe Glu Lys Gln Ala Val 
    530                 535                 540                 


Tyr Gln Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Ser Asn 
545                 550                 555                 560 


Thr Gly Asn Glu Lys Leu Ile Gln Lys Ile Ser Glu Leu Tyr Gly Phe 
                565                 570                 575     


Glu Arg Glu Tyr Ala Ile Ile Ile Ala Asn Ile Ser Phe Gln Pro Asp 
            580                 585                 590         


Tyr Gly Ser Leu Ser Thr Lys Ala Ile Arg Lys Ile Leu Pro His Leu 
        595                 600                 605             


Lys Glu Gly Ile Glu Phe Ser Leu Ala Cys Glu Tyr Ala Gly Tyr Arg 
    610                 615                 620                 


His Ser Lys Asn Ser Leu Thr Lys Glu Glu Lys Asn Lys Lys Ile Leu 
625                 630                 635                 640 


Lys Asp Lys Ile Asp Ile Leu Pro Lys Asn Ser Leu Arg Asn Pro Val 
                645                 650                 655     


Val Glu Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Ala Thr Ile 
            660                 665                 670         


Glu Thr Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu 
        675                 680                 685             


Leu Lys Lys Ser Ala Lys Glu Arg Glu Asp Leu Thr Asn Ala Ile Asn 
    690                 695                 700                 


Lys Ser Thr Leu Asp His Glu Lys Tyr Lys Ala Leu Leu Gln Lys Glu 
705                 710                 715                 720 


Phe Gly Leu Ser Asn Val Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu 
                725                 730                 735     


Tyr Leu Glu Leu Glu His Asn Gly Phe His Thr Leu Tyr Ser Asn Thr 
            740                 745                 750         


Tyr Ile Glu Pro Asn Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His 
        755                 760                 765             


Ile Ile Pro Gln Ser Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr 
    770                 775                 780                 


Ile Glu Ser Arg Ser Ala Asn Ile Glu Lys Gly Asn Glu Thr Ala Phe 
785                 790                 795                 800 


Asp Tyr Val Ser Asn Lys Asn Gly Glu Val Gly Leu Lys Asp Tyr Leu 
                805                 810                 815     


Lys Asn Ile Glu Asp Leu Phe Asn Asn Gly Lys Ile Ser Lys Thr Lys 
            820                 825                 830         


Tyr Asn Lys Leu Lys Met Lys Gly Asp Glu Ile Pro Ser Asp Phe Ile 
        835                 840                 845             


Ala Arg Asp Leu Arg Asp Thr Gln Tyr Ile Ser Lys Lys Ala Lys Ser 
    850                 855                 860                 


Ile Leu Glu Asp Leu Val Lys Asp Val Val Ser Thr Gly Gly Ser Ile 
865                 870                 875                 880 


Thr Asp Arg Leu Arg Glu Asp Trp Gln Leu Ile Asp Ile Met Lys Glu 
                885                 890                 895     


Leu Asn Trp Asp Lys Tyr Asp Lys Leu Gly Leu Thr Glu Val Ile Glu 
            900                 905                 910         


Asn Asn Asp Gly His Lys Ile Asn Arg Ile Lys Asp Trp Thr Lys Arg 
        915                 920                 925             


Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr 
    930                 935                 940                 


Lys Arg Ser Tyr Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Ser Asp 
945                 950                 955                 960 


Lys Ser Gly Ser Ile Tyr Gly Ile Glu Gln Lys Glu Leu Tyr Arg Asp 
                965                 970                 975     


Lys Asn Asn Lys Leu Arg Phe Lys Pro Pro Ile Pro Leu Asp Asp Phe 
            980                 985                 990         


Arg Ala Glu Ala Lys Lys Gln Leu  Glu Asn Thr Leu Val  Ser Ile Lys 
        995                 1000                 1005             


Ala Lys  Asn Lys Val Ala Thr  Ile Asn Ile Asn Asn  Thr Lys Lys 
    1010                 1015                 1020             


Gln Gly  Gly Glu Asn Lys Lys  Lys Gln Leu Thr Pro  Arg Gly Gln 
    1025                 1030                 1035             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Ile Gln Gln  Tyr Val Thr 
    1040                 1045                 1050             


Lys Glu  Glu Lys Val Gly Ala  Asn Phe Asn Lys Glu  Leu Ile Glu 
    1055                 1060                 1065             


Lys Val  Ala Asn Lys Lys Cys  Arg Glu Ala Leu Leu  Lys Arg Leu 
    1070                 1075                 1080             


Ile Glu  Phe Asp Asn Asp Pro  Lys Lys Ala Phe Thr  Arg Lys Asn 
    1085                 1090                 1095             


Ser Leu  Asn Lys Asn Pro Leu  Tyr Ile Asp Thr Ile  Lys Thr Tyr 
    1100                 1105                 1110             


Lys Val  Pro Glu Lys Val Lys  Thr Val Thr Thr Glu  Thr Ile Tyr 
    1115                 1120                 1125             


Thr Ile  Arg Lys Asp Ile Asn  Pro Asp Leu Lys Ile  Asp Lys Val 
    1130                 1135                 1140             


Val Asp  Lys Lys Ile Lys Asn  Ile Leu Glu Leu Arg  Leu Lys Glu 
    1145                 1150                 1155             


Phe Asn  Gly Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Glu Lys 
    1160                 1165                 1170             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1175                 1180                 1185             


Thr Ile  Thr Gly Ile Ser Asn  Ala Ile Ala Leu His  Asp Lys Lys 
    1190                 1195                 1200             


Asp Lys  Glu Gly Asn Phe Ile  Leu Asp Asn Gln Gly  Lys Lys Gln 
    1205                 1210                 1215             


Ala Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Ile 
    1220                 1225                 1230             


Tyr Lys  Asp Glu Lys Gly Asn  Leu Gln Glu Asn Val  Ile Thr Phe 
    1235                 1240                 1245             


Tyr Glu  Ala Val Glu Arg Val  Asn Gln Gly Ile Pro  Ile Ile Asp 
    1250                 1255                 1260             


Lys Thr  Tyr Lys Gln Asn Glu  Gly Trp Gln Phe Leu  Phe Ser Met 
    1265                 1270                 1275             


Lys Gln  Asn Glu Tyr Phe Val  Phe Pro Asn Glu Lys  Thr Gly Phe 
    1280                 1285                 1290             


Asn Ser  Asn Glu Ile Asp Leu  Leu Asn Pro Glu Asn  Tyr His Leu 
    1295                 1300                 1305             


Ile Ser  Pro Asn Leu Phe Arg  Val Gln Lys Ile Ala  Thr Lys Asp 
    1310                 1315                 1320             


Tyr Phe  Phe Arg His His Leu  Glu Thr Asn Val Glu  Asn Ile Thr 
    1325                 1330                 1335             


Thr Thr  Lys Asp Leu Thr Trp  Lys Arg Leu Gly Ile  Asn Gly Ile 
    1340                 1345                 1350             


Ile Asn  Ile Leu Lys Val Arg  Ile Asn His Ile Gly  Gln Ile Val 
    1355                 1360                 1365             


Ser Val  Gly Glu Tyr 
    1370             


<210>  1052
<211>  1373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1052

Met Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Thr Lys Val Asp Glu 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Arg Trp Asp Asp Asp 
            100                 105                 110         


Asn Lys Lys Leu Gly Arg Tyr Pro Ile Phe Gly Asn Lys Ala Asp Val 
        115                 120                 125             


Val Lys Tyr His Gln Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His 
    130                 135                 140                 


Leu Ala Asp Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala 
145                 150                 155                 160 


Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu 
                165                 170                 175     


Leu Asn Ala Glu Asn Thr Asp Val Gln Lys Ile Phe Ala Asp Phe Val 
            180                 185                 190         


Gly Val Tyr Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr 
        195                 200                 205             


Val Asp Ala Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg 
    210                 215                 220                 


Leu Glu Asn Leu Ile Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu 
225                 230                 235                 240 


Phe Gly Asn Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys 
                245                 250                 255     


Met Asn Phe Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp 
            260                 265                 270         


Ser Tyr Glu Glu Asp Leu Gly Glu Leu Leu Gly Lys Ile Gly Asp Asp 
        275                 280                 285             


Tyr Ala Asp Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu 
    290                 295                 300                 


Leu Ser Gly Ile Leu Ile Val Asp Asp Asn Ser Thr Lys Ala Pro Leu 
305                 310                 315                 320 


Ser Ala Ser Met Ile Lys Arg Tyr Val Glu His Gln Glu Asp Leu Glu 
                325                 330                 335     


Lys Leu Lys Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp 
            340                 345                 350         


Ile Phe Lys Asp Lys Asn Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn 
        355                 360                 365             


Gly Val Lys Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Asn Thr Leu Ser 
    370                 375                 380                 


Lys Ile Ala Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp 
385                 390                 395                 400 


Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln 
                405                 410                 415     


Ile His Leu Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr 
            420                 425                 430         


Tyr Pro Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr 
        435                 440                 445             


Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg 
    450                 455                 460                 


Phe Ser Trp Ala Glu Tyr His Ser Asp Glu Lys Ile Thr Pro Trp Asn 
465                 470                 475                 480 


Phe Asp Lys Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr 
                485                 490                 495     


Arg Met Thr Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro 
            500                 505                 510         


Lys His Ser His Val Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr 
        515                 520                 525             


Lys Ile Lys Tyr Val Asn Glu Gln Gly Lys Asp Ser Phe Phe Asp Ser 
    530                 535                 540                 


Asn Met Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys 
545                 550                 555                 560 


Val Thr Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu 
                565                 570                 575     


Tyr Arg Ile Lys Asp Leu Ile Gly Leu Asp Lys Glu Asn Lys Ser Phe 
            580                 585                 590         


Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys 
        595                 600                 605             


Ala Phe Leu Asp Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile 
    610                 615                 620                 


Lys Thr Leu Thr Leu Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu 
625                 630                 635                 640 


Gln Lys Tyr Ser Asp Ile Phe Thr Ala Asp Gln Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn 
            660                 665                 670         


Gly Ile Arg Asn Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile 
        675                 680                 685             


Asp Asp Gly Ser Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp 
    690                 695                 700                 


Thr Leu Pro Phe Lys Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp 
705                 710                 715                 720 


Val Asp Asp Ile Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Lys Val Met Gly Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu 
    770                 775                 780                 


Lys Lys Leu Gln Asn Ser Leu Lys Glu Leu Gly Ser Asn Ile Leu Asn 
785                 790                 795                 800 


Glu Glu Lys Pro Ser Tyr Ile Glu Asp Lys Val Glu Asn Ser His Leu 
                805                 810                 815     


Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met 
            820                 825                 830         


Tyr Thr Gly Asp Glu Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile 
        835                 840                 845             


Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn 
    850                 855                 860                 


Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val 
865                 870                 875                 880 


Pro Ser Leu Asp Ile Val Arg Ala Arg Lys Ala Glu Trp Val Arg Leu 
                885                 890                 895     


Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys 
            900                 905                 910         


Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala Gly Phe Ile Lys 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile 
    930                 935                 940                 


Leu Asp Ala Arg Phe Asn Thr Glu Ser Asp Glu Asn Asp Lys Val Ile 
945                 950                 955                 960 


Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe 
                965                 970                 975     


Arg Lys Asp Phe Glu Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His 
            980                 985                 990         


His Ala His Asp Ala Tyr Leu Asn  Ala Val Val Gly Thr  Ala Leu Leu 
        995                 1000                 1005             


Lys Lys  Tyr Pro Lys Leu Ala  Ser Glu Phe Val Tyr  Gly Glu Tyr 
    1010                 1015                 1020             


Lys Lys  Tyr Asp Ile Arg Lys  Phe Ile Thr Asn Ser  Ser Asp Lys 
    1025                 1030                 1035             


Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn Leu Met  Asn Phe Phe 
    1040                 1045                 1050             


Lys Arg  Val Ile Arg Tyr Ser  Asn Gly Lys Val Ile  Val Arg Pro 
    1055                 1060                 1065             


Val Val  Glu Tyr Ser Lys Asp  Thr Glu Asp Ile Ala  Trp Asp Lys 
    1070                 1075                 1080             


Lys Ser  Asn Phe Arg Thr Ile  Cys Lys Val Leu Ser  Tyr Pro Gln 
    1085                 1090                 1095             


Val Asn  Ile Val Lys Lys Val  Glu Thr Gln Thr Gly  Gly Phe Ser 
    1100                 1105                 1110             


Lys Glu  Ser Ile Leu Pro Lys  Gly Asp Ser Asp Lys  Leu Ile Pro 
    1115                 1120                 1125             


Arg Lys  Thr Lys Lys Ala Tyr  Trp Asp Thr Lys Lys  Tyr Gly Gly 
    1130                 1135                 1140             


Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val Phe Val  Val Ala Asp 
    1145                 1150                 1155             


Val Glu  Lys Gly Lys Ala Lys  Lys Leu Lys Thr Val  Lys Glu Leu 
    1160                 1165                 1170             


Val Gly  Ile Ser Ile Met Glu  Arg Ser Phe Phe Glu  Glu Asn Pro 
    1175                 1180                 1185             


Val Glu  Phe Leu Glu Asn Lys  Gly Tyr His Asn Ile  Arg Glu Asp 
    1190                 1195                 1200             


Lys Leu  Ile Lys Leu Pro Lys  Tyr Ser Leu Phe Glu  Phe Glu Gly 
    1205                 1210                 1215             


Gly Lys  Arg Arg Leu Leu Ala  Ser Ala Ser Glu Leu  Gln Lys Gly 
    1220                 1225                 1230             


Asn Glu  Met Val Ile Pro Gly  His Leu Val Lys Leu  Leu Tyr His 
    1235                 1240                 1245             


Ala Gln  Arg Ile Asn Ser Phe  Asn Ser Thr Lys Tyr  Leu Asp Tyr 
    1250                 1255                 1260             


Val Ser  Ala His Lys Lys Glu  Phe Glu Lys Val Leu  Ser Cys Val 
    1265                 1270                 1275             


Glu Asp  Phe Ala Asn Leu Tyr  Val Asp Val Glu Lys  Asn Leu Ser 
    1280                 1285                 1290             


Lys Ile  Arg Ala Val Ala Asp  Ser Met Asp Asn Phe  Ser Ile Glu 
    1295                 1300                 1305             


Glu Ile  Ser Asn Ser Phe Ile  Asn Leu Leu Thr Leu  Thr Ala Leu 
    1310                 1315                 1320             


Gly Ala  Pro Ala Asp Phe Asn  Phe Leu Gly Glu Lys  Ile Pro Arg 
    1325                 1330                 1335             


Lys Arg  Tyr Thr Ser Thr Lys  Glu Cys Leu Asn Ala  Thr Leu Ile 
    1340                 1345                 1350             


His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser 
    1355                 1360                 1365             


Lys Ile  Gly Glu Glu 
    1370             


<210>  1053
<211>  1373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1053

Met Glu Asn Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Arg Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Ala Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Ile Pro Glu Asp Lys 
            100                 105                 110         


Lys Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ile Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Asp Thr Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asn Asn Thr Phe Glu Gly Asn Ser Leu Ser Gly Gln Asn Val Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Arg Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Val Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Thr Leu 
                325                 330                 335     


Lys Gln Phe Ile Lys Thr Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ser Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Ala Asp Tyr Phe Leu Glu Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Lys Ala Ser Ser Ala Glu Ser Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Arg 
    530                 535                 540                 


Gln Lys Lys Asp Ile Phe Tyr Thr Leu Phe Lys Ala Glu Asp Lys Arg 
545                 550                 555                 560 


Lys Val Thr Glu Lys Asp Ile Ile Gln Tyr Leu His Thr Val Asp Gly 
                565                 570                 575     


Tyr Asp Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser 
            580                 585                 590         


Leu Ser Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe 
        595                 600                 605             


Met Asp Asp Pro Asn Asn Glu Glu Ile Leu Glu Asn Ile Val His Thr 
    610                 615                 620                 


Leu Thr Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln 
625                 630                 635                 640 


Tyr Asp Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Lys Leu Ser Ser Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Gln Thr Gly Lys Thr Ile Leu Asp Tyr Leu Met Asp Asp 
        675                 680                 685             


Gly Tyr Asn Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Glu Leu 
    690                 695                 700                 


Ser Phe Lys Glu Ile Ile Lys Lys Ala Gln Val Val Gly Lys Thr Asp 
705                 710                 715                 720 


Asp Val Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Ile Lys Leu Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly His Glu Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg 
    770                 775                 780                 


Ile Glu Asp Ser Leu Lys Ile Leu Ala Ser Gly Leu Asn Ala Lys Ile 
785                 790                 795                 800 


Leu Lys Glu His Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Lys Pro 
            820                 825                 830         


Leu Asp Ile Asn Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Val Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser 
    850                 855                 860                 


Leu Lys Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val 
865                 870                 875                 880 


Val Glu Lys Met Lys Thr Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu 
                885                 890                 895     


Ile Ser Tyr Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Asp Glu Arg Asp Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Tyr 
    930                 935                 940                 


Asn Thr Glu Val Asn Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile 
945                 950                 955                 960 


Ile Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Arg 
                965                 970                 975     


Leu Tyr Lys Ile Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Lys Lys  Tyr Pro Lys 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Gln Lys  Tyr Asp Leu 
    1010                 1015                 1020             


Lys Arg  Tyr Ile Ser Arg Ser  Lys Asp Pro Lys Glu  Ile Glu Lys 
    1025                 1030                 1035             


Ala Thr  Glu Lys Tyr Phe Phe  Tyr Ser Asn Leu Leu  Asn Phe Phe 
    1040                 1045                 1050             


Lys Glu  Glu Val His Tyr Ala  Asp Gly Thr Ile Val  Lys Arg Glu 
    1055                 1060                 1065             


Asn Ile  Glu Tyr Ser Lys Asp  Thr Gly Glu Ile Ala  Trp Asn Lys 
    1070                 1075                 1080             


Glu Lys  Asp Phe Ala Thr Ile  Lys Lys Val Leu Ser  Leu Pro Gln 
    1085                 1090                 1095             


Val Asn  Ile Val Lys Lys Arg  Glu Val Gln Thr Gly  Gly Phe Ser 
    1100                 1105                 1110             


Lys Glu  Ser Ile Leu Pro Lys  Gly Asn Ser Asp Lys  Leu Ile Pro 
    1115                 1120                 1125             


Arg Lys  Thr Lys Asp Ile Leu  Trp Asp Thr Thr Lys  Tyr Gly Gly 
    1130                 1135                 1140             


Phe Asp  Ser Pro Val Ile Ala  Tyr Ser Ile Leu Leu  Ile Ala Asp 
    1145                 1150                 1155             


Ile Glu  Lys Gly Lys Ala Lys  Arg Leu Lys Thr Val  Lys Thr Leu 
    1160                 1165                 1170             


Val Gly  Ile Thr Ile Met Glu  Lys Ala Thr Phe Glu  Lys Ser Pro 
    1175                 1180                 1185             


Ile Ala  Phe Leu Glu Asn Lys  Gly Tyr His Asn Val  Arg Lys Glu 
    1190                 1195                 1200             


Asn Ile  Leu Cys Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Lys Asn 
    1205                 1210                 1215             


Gly Arg  Arg Arg Met Leu Ala  Ser Ala Lys Glu Leu  Gln Lys Gly 
    1220                 1225                 1230             


Asn Glu  Ile Val Leu Pro Val  His Leu Thr Thr Leu  Leu Tyr His 
    1235                 1240                 1245             


Ala Lys  Asn Ile His Arg Leu  Asp Glu Pro Glu His  Leu Glu Tyr 
    1250                 1255                 1260             


Ile Gln  Lys His Arg Asn Glu  Phe Lys Gly Leu Leu  Asn Leu Val 
    1265                 1270                 1275             


Ser Glu  Phe Ser Gln Lys Tyr  Val Leu Ala Asp Ala  Asn Leu Glu 
    1280                 1285                 1290             


Lys Ile  Lys Asn Leu Tyr Ala  Asp Asn Glu Gln Ala  Asp Ile Glu 
    1295                 1300                 1305             


Ile Leu  Ala Asn Ser Phe Ile  Asn Leu Leu Thr Phe  Thr Ala Leu 
    1310                 1315                 1320             


Gly Ala  Pro Ala Ala Phe Lys  Phe Phe Gly Lys Asp  Val Asp Arg 
    1325                 1330                 1335             


Lys Arg  Tyr Thr Thr Val Ser  Glu Ile Leu Asn Ala  Thr Leu Ile 
    1340                 1345                 1350             


His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser 
    1355                 1360                 1365             


Lys Leu  Gly Glu Asp 
    1370             


<210>  1054
<211>  1374
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1054

Met Glu Thr Lys Phe Thr Tyr Tyr Leu Gly Leu Asp Met Gly Thr Asp 
1               5                   10                  15      


Ser Leu Gly Trp Ala Val Thr Asp Ser Ser Tyr His Ile Leu Arg Ala 
            20                  25                  30          


Lys Gly Lys Asp Leu Trp Gly Val Arg Leu Phe Pro Ser Ala Glu Thr 
        35                  40                  45              


Ser Val Glu Arg Arg Thr Tyr Arg Val Asn Arg Arg Arg Arg Gln Arg 
    50                  55                  60                  


Glu Val Ala Arg Ile Gly Ala Leu Lys Glu Leu Phe Ala Asp Glu Ile 
65                  70                  75                  80  


Glu Lys Val Asp Pro Gly Phe Phe Val Arg Leu Asp Glu Ser Lys Phe 
                85                  90                  95      


His Met Glu Asp Arg Ser Glu Asn Asn Lys Gln Lys Tyr Gly Leu Phe 
            100                 105                 110         


Ala Asp Lys Leu Phe Ser Asp Ala Gly Tyr Tyr Lys Lys Tyr Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Lys Glu Leu Leu Glu Ser Glu Glu Pro His Asp 
    130                 135                 140                 


Val Arg Leu Val Tyr Leu Ala Leu Leu Asn Met Phe Lys His Arg Gly 
145                 150                 155                 160 


His Phe Leu Asn Ser Ser Leu Gln Ile Glu Gly Glu Asn Ser Asn Ile 
                165                 170                 175     


Ala Glu Ser Tyr Gln Met Phe Val Asp Thr Leu Thr Asp Glu Asp Leu 
            180                 185                 190         


Asp Phe Pro Glu Gln Val Ser Gly Glu Arg Leu Gln Glu Ile Phe Arg 
        195                 200                 205             


Ser Ser Asp Ser Arg Lys Lys Lys Val Glu Asn Leu Leu Ala Glu Met 
    210                 215                 220                 


Asn Leu Ser Lys Lys Gln Lys Arg Glu Ile Gln Phe Val Asn Leu Ile 
225                 230                 235                 240 


Cys Gly Leu Lys Gly Lys Leu Ser Glu Leu Phe Pro Asp Lys Glu Tyr 
                245                 250                 255     


Asp Glu Glu Gln Lys Lys Ile Ala Phe Ser Phe Gln Asp Ser Asp Tyr 
            260                 265                 270         


Glu Asp Lys Glu Asn Thr Ile Gln Glu Leu Leu Asp Glu Ser Glu Met 
        275                 280                 285             


Asn Met Ile Tyr Ala Val Lys Ala Val His Asp Ser Gly Val Leu Ser 
    290                 295                 300                 


Gly Ile Met Asn Gly Glu Arg Tyr Leu Ser Val Ala Arg Val Ala Ser 
305                 310                 315                 320 


Tyr Glu Lys His Lys Lys Asp Leu Gln Ile Leu Lys Asp Ile Leu Lys 
                325                 330                 335     


Lys Tyr Asp Thr Lys Glu Tyr Phe Glu Met Phe Arg Asn Glu Ser Ala 
            340                 345                 350         


Lys Ser Glu Ser Tyr Ser Ala Tyr Val Gly Ser Val Lys Tyr His Gly 
        355                 360                 365             


Thr Phe Arg Arg Gly Leu Ser Gly Gly Lys Gln Glu Asp Leu Tyr Lys 
    370                 375                 380                 


His Ile Lys Lys Val Leu Gly Lys Phe Pro Gln Asp Asp Glu Gly Val 
385                 390                 395                 400 


Thr Tyr Ile Leu Glu Glu Ile Glu Thr Glu Ser Phe Leu Pro Lys Gln 
                405                 410                 415     


Leu Thr Ala Ser Asn Gly Val Ile Pro Asn Gln Val His Ala Arg Glu 
            420                 425                 430         


Met Leu Ala Ile Leu Lys His Ala Glu Leu Tyr Leu Pro Phe Leu Gln 
        435                 440                 445             


Glu Lys Asp Asp Thr Gly Leu Ser Val Ser Glu Lys Ile Leu Lys Leu 
    450                 455                 460                 


Phe Met Phe Gln Ile Pro Tyr Tyr Val Gly Pro Leu Gly Gln Glu Tyr 
465                 470                 475                 480 


Lys Asp Lys Lys Gly Tyr Asn Val Trp Ala Glu Arg Lys Ala Ser Gly 
                485                 490                 495     


Lys Ile Tyr Pro Trp Asn Phe Glu Asp Lys Ile Asp Val Lys Lys Ser 
            500                 505                 510         


Ala Glu Lys Phe Ile Gly Arg Met Val Arg Arg Cys Thr Tyr Leu Ser 
        515                 520                 525             


Gly Glu Gln Ala Leu Pro Lys Gln Ser Leu Leu Tyr Glu Lys Phe Gln 
    530                 535                 540                 


Val Leu Asn Glu Leu Asn Asn Leu Arg Ile Arg Gly Glu Lys Ile Pro 
545                 550                 555                 560 


Val Ser Leu Lys Gln Glu Ile Tyr His Asp Leu Phe Gly Asp Gly Lys 
                565                 570                 575     


Lys Val Ser Met Thr Arg Leu Thr Asn Tyr Leu Leu Gln Asn Gly Val 
            580                 585                 590         


Ile Ser Gly Glu Glu Lys Asp Ser Cys Ile Ser Gly Ile Asp Gly Gly 
        595                 600                 605             


Phe His Asn Ser Leu Ser Ser Val Gly Lys Phe Lys Gly Val Phe Gly 
    610                 615                 620                 


Asp Thr Val His Thr Asp Glu His Arg Lys Met Ile Glu Asp Ile Ile 
625                 630                 635                 640 


Phe Trp Gly Thr Val Phe Gly Asp Gly Lys Lys Leu Leu Lys Glu Lys 
                645                 650                 655     


Val Glu Glu Val Tyr Gly Asn Gln Leu Thr Glu Ala Gln Lys Lys Arg 
            660                 665                 670         


Ile Phe Gly Phe Lys Phe Ser Ser Trp Gly Arg Leu Ser Lys Ala Phe 
        675                 680                 685             


Leu Thr Met Glu Gly Ala Ser Lys Asp Asp Gly Glu Ile Lys Gly Leu 
    690                 695                 700                 


Ile Gln Ala Met Trp Asp Thr Asn Cys Asn Leu Met Glu Leu Leu Ser 
705                 710                 715                 720 


Asp Arg Phe Thr Tyr Lys Asp Lys Leu Asn Glu Met Val Thr Thr Ala 
                725                 730                 735     


Glu Lys Pro Leu Cys Glu Trp Lys Ile Glu Asp Leu Asp Glu Met Tyr 
            740                 745                 750         


Leu Ser Ala Pro Val Lys Arg Met Val Trp Gln Thr Ile Arg Val Val 
        755                 760                 765             


Asp Glu Val Gln Gln Ile Ile Gly Cys Pro Pro Lys Arg Ile Phe Val 
    770                 775                 780                 


Glu Met Thr Arg Ser Asp Gly Glu Lys Gly Lys Arg Thr Val Ser Arg 
785                 790                 795                 800 


Gln Lys Lys Leu Val Asp Leu Tyr Lys Ala Leu Gly Lys Glu Gly Lys 
                805                 810                 815     


Thr Trp Ser Glu Glu Leu Ser Asp Thr Pro Glu Ser Gln Phe Arg Ile 
            820                 825                 830         


Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Met Gly Lys Cys Met Tyr Thr 
        835                 840                 845             


Gly Glu Ala Ile Glu Leu Glu Gln Leu Met Asn Asp Asn Leu Tyr Asp 
    850                 855                 860                 


Ile Asp His Ile Tyr Pro Arg His Phe Ile Lys Asp Asp Asn Ile Ser 
865                 870                 875                 880 


Asn Asn Leu Val Leu Val Lys Lys Glu Lys Asn Ala His Lys Ser Asp 
                885                 890                 895     


Ser Phe Pro Ile Glu Pro Glu Ile Arg Asn Lys Met Met Pro Phe Trp 
            900                 905                 910         


Lys Ser Leu Arg Lys Lys Gly Phe Leu Thr Glu Glu Lys Tyr Asn Arg 
        915                 920                 925             


Leu Thr Arg Met Ser Ala Phe Thr Asp Glu Glu Lys Ala Ala Phe Ile 
    930                 935                 940                 


Gly Arg Gln Leu Val Glu Thr Ser Gln Gly Thr Lys Ala Ile Thr Gln 
945                 950                 955                 960 


Ile Phe Glu Gln Ala Phe Ala Asn Ser Glu Ile Val Phe Ser Lys Ala 
                965                 970                 975     


Ser Val Val Ser Ala Phe Arg Gln Lys Phe Asp Cys Tyr Lys Val Arg 
            980                 985                 990         


Cys Val Asn Asn Tyr His His Ala  His Asp Ala Tyr Leu  Asn Ile Val 
        995                 1000                 1005             


Ala Gly  Asn Thr Tyr Arg Val  Lys Phe Thr Ser Asn  Pro Leu Asn 
    1010                 1015                 1020             


Phe Ile  Lys Glu Ala Lys Lys  Asn Pro Asn Ser Arg  Glu Asn Arg 
    1025                 1030                 1035             


Tyr Asn  Met Asp Lys Ile Phe  Glu Trp Asp Val Thr  Arg Asn Gly 
    1040                 1045                 1050             


Glu Thr  Ala Trp Val Ala Gln  Thr Lys Glu His Pro  Gly Thr Ile 
    1055                 1060                 1065             


Gln Leu  Val Lys Lys Tyr Leu  Asn Lys Asn Ser Pro  Leu Ile Thr 
    1070                 1075                 1080             


Lys Met  Val Tyr Glu Lys His  Gly Gly Ile Thr Gln  Lys Ala Thr 
    1085                 1090                 1095             


Ile Trp  Ser Ala Lys Lys Ala  Glu Ser Gly Asn Thr  Asp Ala Tyr 
    1100                 1105                 1110             


Ile Pro  Val Lys Met Asn Asp  Pro Arg Leu Ala Asp  Val Thr Lys 
    1115                 1120                 1125             


Tyr Gly  Gly Val Thr Thr Ile  Ala Val Ala Gly Tyr  Thr Leu Leu 
    1130                 1135                 1140             


Glu Tyr  Lys Ala Gly Gly Lys  Thr Val Arg Ser Leu  Glu Ala Leu 
    1145                 1150                 1155             


Pro Ile  Tyr Leu Gly Gly Ser  Lys Lys Leu Thr Glu  Glu Gln Met 
    1160                 1165                 1170             


Val Glu  Tyr Phe Ser Asn Val  Leu Gln Gln Glu Asn  Lys Glu Lys 
    1175                 1180                 1185             


Val Val  Ser Asp Val Ile Ile  Arg Lys Lys Met Ile  Pro Ser Gly 
    1190                 1195                 1200             


Ser Leu  Ile Lys Tyr Asn Gly  Phe Tyr Tyr Tyr Leu  Ala Gly Lys 
    1205                 1210                 1215             


Thr Gly  Asp Arg Ile Ile Leu  Ile Ser Ala Val Gln  Leu Cys Leu 
    1220                 1225                 1230             


Pro Met  Asp Thr Met Leu Tyr  Ile Lys Lys Ile Glu  Lys Ala Cys 
    1235                 1240                 1245             


Ser Thr  Gly Asn Phe Glu Glu  Lys Asp Lys Asn Gly  Lys Gln Val 
    1250                 1255                 1260             


Leu Ser  Lys Gln Gln Asn Glu  Phe Val Tyr Glu Leu  Ile Ile Gln 
    1265                 1270                 1275             


Lys Tyr  Gln Gln Ser Ile Phe  Lys Lys Lys Val Gly  Ala Ile Gly 
    1280                 1285                 1290             


Asp Thr  Ile Ile Glu Lys Arg  Glu Lys Phe Ala Glu  Ile Ser Leu 
    1295                 1300                 1305             


Glu Glu  Gln Cys Lys Val Leu  Met Gln Ile Phe Leu  Asn Phe Gln 
    1310                 1315                 1320             


Ser Gly  Ile Gly Val Glu Leu  Lys Ile Ile Glu Gly  Gly Lys Lys 
    1325                 1330                 1335             


Ser Gly  Val Thr Tyr Leu Asn  Lys Lys Val Met Asn  Val Gln Glu 
    1340                 1345                 1350             


Leu Lys  Leu Ile Asp Gln Ser  Val Thr Gly Val Phe  Ser Arg Glu 
    1355                 1360                 1365             


Ile Asp  Leu Leu Thr Ile 
    1370                 


<210>  1055
<211>  1374
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1055

Met Lys Lys Arg Tyr Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Ile Asn Glu Asp Tyr Lys Val Pro Ala Lys 
            20                  25                  30          


Lys Met Thr Val Phe Gly Asn Thr Asp Arg Lys Thr Ile Lys Lys Asn 
        35                  40                  45              


Leu Leu Gly Thr Val Leu Phe Asp Ser Gly Glu Thr Ala Gln Ala Arg 
    50                  55                  60                  


Arg Leu Lys Arg Thr Asn Arg Arg Arg Tyr Thr Arg Arg Arg Tyr Arg 
65                  70                  75                  80  


Leu Cys Gln Leu Gln Asn Ile Phe Ala Thr Glu Met Val Lys Val Asp 
                85                  90                  95      


Asp Thr Phe Phe Gln Arg Leu Ser Glu Ser Phe Phe Tyr Tyr Gln Asp 
            100                 105                 110         


Lys Ala Phe Asp Lys His Pro Ile Phe Gly Asn Ser Lys Glu Glu Arg 
        115                 120                 125             


Ala Tyr His Lys Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys Asp Leu 
    130                 135                 140                 


Ala Asp Arg Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu 
145                 150                 155                 160 


Ser His Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Lys Leu 
                165                 170                 175     


Asn Ser Glu Asn Thr Asp Val Gln Lys Leu Phe Ile Ala Leu Val Thr 
            180                 185                 190         


Val Tyr Asn Leu Leu Phe Glu Glu Glu Pro Ile Ala Gly Glu Thr Cys 
        195                 200                 205             


Asp Ala Lys Ala Leu Leu Thr Ala Lys Thr Ser Lys Ser Lys Arg Leu 
    210                 215                 220                 


Glu Ser Leu Ile Ser Glu Phe Pro Gly Gln Lys Lys Asn Gly Leu Phe 
225                 230                 235                 240 


Gly Asn Leu Leu Ala Leu Ala Leu Gly Leu Arg Pro Asn Phe Lys Ser 
                245                 250                 255     


Asn Phe Gly Leu Ser Glu Asp Ala Lys Leu Gln Ile Thr Lys Asp Thr 
            260                 265                 270         


Tyr Glu Glu Glu Leu Asp Asn Leu Leu Ala Glu Ile Gly Asp His Tyr 
        275                 280                 285             


Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu 
    290                 295                 300                 


Ser Asp Ile Leu Thr Leu Ser Asp Glu Asn Thr Arg Ala Pro Leu Ser 
305                 310                 315                 320 


Ala Ser Met Ile Lys Arg Tyr Glu Glu His Gln Glu Asp Leu Ala Leu 
                325                 330                 335     


Leu Lys Lys Leu Val Lys Glu Gln Met Pro Glu Lys Tyr Trp Glu Ile 
            340                 345                 350         


Phe Ser Asn Ala Lys Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys 
        355                 360                 365             


Val Ser Gln Glu Asp Phe Tyr Arg Tyr Ile Lys Pro Ile Leu Ser Arg 
    370                 375                 380                 


Leu Lys Gly Gly Asp Glu Phe Leu Ala Lys Ile Asp Arg Asp Asp Phe 
385                 390                 395                 400 


Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile 
                405                 410                 415     


His Leu Lys Glu Leu His Ala Ile Leu Arg Arg Gln Glu Lys Tyr Tyr 
            420                 425                 430         


Pro Phe Leu Ala Glu Gln Lys Glu Lys Ile Glu Gln Leu Leu Cys Phe 
        435                 440                 445             


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gly Asn Ser Ser 
    450                 455                 460                 


Phe Ala Trp Leu Lys Arg Arg Ser Asp Glu Pro Ile Thr Pro Trp Asn 
465                 470                 475                 480 


Phe Lys Asp Val Val Asp Glu Glu Ala Ser Ala Gln Ala Phe Ile Glu 
                485                 490                 495     


Gly Met Thr Asn Tyr Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro 
            500                 505                 510         


Lys His Ser Pro Leu Tyr Glu Met Phe Thr Val Tyr Asn Glu Leu Thr 
        515                 520                 525             


Lys Val Lys Tyr Ile Ala Glu Asn Met Thr Lys Pro Leu Tyr Leu Ser 
    530                 535                 540                 


Ala Glu Gln Lys Glu Ala Ile Ile Asp His Leu Phe Lys Gln Thr Arg 
545                 550                 555                 560 


Lys Val Thr Val Lys Asp Leu Lys Glu Lys Tyr Phe Ser Gln Ile Glu 
                565                 570                 575     


Gly Leu Glu Asn Val Asp Val Thr Gly Val Glu Gly Ala Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr Asn Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala 
        595                 600                 605             


Phe Leu Asp Asp Glu Ala Asn Ala Glu Ile Leu Glu Glu Ile Val Leu 
    610                 615                 620                 


Ile Leu Thr Leu Phe Gln Asp Glu Lys Leu Ile Glu Lys Arg Leu Ala 
625                 630                 635                 640 


Lys Tyr Ala Asn Leu Phe Glu Lys Ser Val Leu Lys Lys Leu Arg Lys 
                645                 650                 655     


Arg His Tyr Arg Gly Trp Gly Arg Leu Ser Arg Gln Leu Ile Asp Gly 
            660                 665                 670         


Met Lys Asp Lys Ala Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala 
        675                 680                 685             


Asp Asp Phe Ala Asn Arg Asn Phe Ile Gln Leu Ile Asn Asp Ser Ser 
    690                 695                 700                 


Leu Asp Phe Glu Lys Leu Ile Asp Asp Ala Gln Lys Lys Ala Ile Lys 
705                 710                 715                 720 


Arg Glu Ser Leu Thr Glu Ala Val Ala Asn Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Val Val Asp Glu Ile Val 
            740                 745                 750         


Lys Val Met Gly His Asn Pro Asp Asn Ile Val Ile Glu Met Ser Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Ala Gln Gly Leu Lys Asn Ala Arg Gln Arg Leu 
    770                 775                 780                 


Lys Lys Ile Lys Glu Val His Lys Lys Thr Gly Ser Arg Ile Leu Glu 
785                 790                 795                 800 


Asp Asn Ser Glu Arg Ile Thr Asn Leu Thr Leu Gln Asp Asn Arg Leu 
                805                 810                 815     


Tyr Leu Tyr Leu Leu Gln Asp Gly Lys Asp Met Tyr Thr Gly Gln Asp 
            820                 825                 830         


Leu Asp Ile Asn Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ser Phe Ile Lys Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Thr 
    850                 855                 860                 


Gln Lys Ala Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val 
865                 870                 875                 880 


Val Arg Asp Met Lys Asp Arg Val Trp Arg Arg Gln Leu Ala Asn Gly 
                885                 890                 895     


Ala Ile Ser Arg Gln Lys Phe Asp His Leu Thr Lys Ala Glu Arg Gly 
            900                 905                 910         


Gly Leu Ala Asp Ser Asp Lys Ala Arg Phe Leu Arg Arg Gln Leu Val 
        915                 920                 925             


Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Leu Leu Asp Ser Arg 
    930                 935                 940                 


Phe Asn Ser Lys Ser Asn Gln Asn Lys Lys Leu Ala Arg Asn Val Lys 
945                 950                 955                 960 


Ile Ile Thr Leu Lys Ser Lys Ile Val Ser Asp Phe Arg Lys Asp Phe 
                965                 970                 975     


Gly Leu Tyr Lys Leu Arg Glu Val Asn Asn Tyr His His Ala His Asp 
            980                 985                 990         


Ala Tyr Leu Asn Ala Val Val Gly  Thr Ala Leu Leu Lys  Lys Tyr Pro 
        995                 1000                 1005             


Lys Leu  Glu Ala Glu Phe Val  Tyr Gly Asp Tyr Lys  His Phe Asp 
    1010                 1015                 1020             


Leu Val  Lys Leu Ile Ser Lys  Ser Asp Pro Ser Leu  Gly Lys Ala 
    1025                 1030                 1035             


Thr Ala  Lys Val Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1040                 1045                 1050             


Glu Glu  Leu Ser Leu Ala Asp  Gly Thr Leu Met Lys  Arg Pro Val 
    1055                 1060                 1065             


Ile Glu  Thr Asn Thr Glu Thr  Gly Glu Val Val Trp  Asp Lys Val 
    1070                 1075                 1080             


Lys Asp  Phe Lys Thr Ile Arg  Lys Val Leu Ser Tyr  Pro Gln Val 
    1085                 1090                 1095             


Asn Ile  Val Lys Lys Thr Glu  Ile Gln Ser Gly Ala  Phe Ser Lys 
    1100                 1105                 1110             


Glu Ser  Val Leu Ser Lys Gly  Asn Ser Asp Lys Leu  Ile Glu Arg 
    1115                 1120                 1125             


Lys Lys  Gly Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1130                 1135                 1140             


Asn Thr  Ala Tyr Ser Ile Phe  Val Val Ala Lys Val  Ala Lys Arg 
    1145                 1150                 1155             


Lys Ala  Gln Lys Leu Lys Thr  Val Lys Glu Ile Val  Gly Ile Thr 
    1160                 1165                 1170             


Ile Met  Glu Gln Ala Glu Tyr  Glu Lys Asp Asn Ile  Ala Phe Leu 
    1175                 1180                 1185             


Glu Lys  Lys Gly Tyr Gln Asp  Ile Gln Glu Lys Leu  Leu Ile Lys 
    1190                 1195                 1200             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Arg Arg 
    1205                 1210                 1215             


Leu Leu  Ala Ser Ala Asn Glu  Phe Gln Lys Gly Asn  Glu Leu Ala 
    1220                 1225                 1230             


Leu Ser  Gly Lys Tyr Met Lys  Phe Leu Tyr Leu Ala  Ser Arg Tyr 
    1235                 1240                 1245             


Asp Lys  Leu Ser Ser Lys Ile  Glu Ser Glu Gln Gln  Lys Lys Leu 
    1250                 1255                 1260             


Phe Val  Glu Gln His Leu His  Tyr Phe Asp Glu Ile  Leu Asp Ile 
    1265                 1270                 1275             


Val Val  Lys His Ala Thr Cys  Tyr Ile Lys Ala Glu  Asn Asn Leu 
    1280                 1285                 1290             


Lys Lys  Ile Ile Ser Leu Tyr  Lys Lys Lys Glu Ala  Tyr Ser Ile 
    1295                 1300                 1305             


Asn Glu  Gln Ala Leu Asn Met  Leu Asn Leu Phe Ile  Phe Thr Ser 
    1310                 1315                 1320             


Leu Gly  Ala Pro Ser Thr Phe  Val Phe Phe Asp Glu  Thr Ile Asp 
    1325                 1330                 1335             


Arg Lys  Arg Tyr Thr Thr Ser  Ser Asp Val Leu Asn  Gly Ile Leu 
    1340                 1345                 1350             


Ile Gln  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1355                 1360                 1365             


Ser Arg  Phe Gly Gly Asp 
    1370                 


<210>  1056
<211>  1374
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1056

Met Lys Lys Gln Lys Phe Ser Asp Tyr Tyr Leu Gly Phe Asp Ile Gly 
1               5                   10                  15      


Thr Asn Ser Val Gly Trp Cys Val Thr Asp Leu Asp Tyr Asn Val Leu 
            20                  25                  30          


Arg Phe Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Asp Glu Ala 
        35                  40                  45              


Lys Thr Ala Ala Glu Arg Arg Val Gln Arg Asn Ser Arg Arg Arg Leu 
    50                  55                  60                  


Lys Arg Arg Lys Trp Arg Leu Asn Leu Leu Glu Glu Ile Phe Ser Asp 
65                  70                  75                  80  


Glu Ile Met Lys Ile Asp Ser Asn Phe Phe Arg Arg Leu Lys Glu Ser 
                85                  90                  95      


Ser Leu Trp Leu Glu Asp Lys Asn Ser Lys Glu Lys Phe Thr Leu Phe 
            100                 105                 110         


Asn Asp Asp Asn Tyr Lys Asp Tyr Asp Phe Tyr Lys Gln Tyr Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Asp Glu Leu Ile Lys Asn Pro Glu Lys Lys Asp 
    130                 135                 140                 


Ile Arg Leu Ile Tyr Leu Ala Leu His Ser Ile Phe Lys Ser Arg Gly 
145                 150                 155                 160 


His Phe Leu Phe Glu Gly Gln Asn Leu Lys Glu Ile Lys Asn Phe Glu 
                165                 170                 175     


Thr Leu Tyr Asn Asn Leu Ile Ser Phe Leu Glu Asp Asn Gly Ile Asn 
            180                 185                 190         


Lys Ser Ile Asp Lys Asp Asn Ile Glu Lys Leu Glu Lys Ile Ile Cys 
        195                 200                 205             


Asp Ser Gly Lys Gly Leu Lys Asp Lys Glu Lys Glu Phe Lys Gly Ile 
    210                 215                 220                 


Phe Asn Ser Asp Lys Gln Leu Val Ala Ile Phe Lys Leu Ser Val Gly 
225                 230                 235                 240 


Ser Ser Val Ser Leu Asn Asp Leu Phe Asp Thr Asp Glu Tyr Lys Lys 
                245                 250                 255     


Glu Glu Val Glu Lys Glu Lys Ile Ser Phe Arg Glu Gln Ile Tyr Glu 
            260                 265                 270         


Asp Asp Lys Pro Ile Tyr Tyr Ser Ile Leu Gly Glu Lys Ile Glu Leu 
        275                 280                 285             


Leu Asp Ile Ala Lys Ser Phe Tyr Asp Phe Met Val Leu Asn Asn Ile 
    290                 295                 300                 


Leu Ser Asp Ser Asn Tyr Ile Ser Glu Ala Lys Val Lys Leu Tyr Glu 
305                 310                 315                 320 


Glu His Lys Lys Asp Leu Lys Asn Leu Lys Tyr Ile Ile Arg Lys Tyr 
                325                 330                 335     


Asn Lys Glu Asn Tyr Asp Lys Leu Phe Lys Asp Lys Asn Glu Asn Asn 
            340                 345                 350         


Tyr Pro Ala Tyr Ile Gly Leu Asn Lys Glu Lys Asp Lys Lys Glu Val 
        355                 360                 365             


Val Glu Lys Ser Arg Leu Lys Ile Asp Asp Leu Ile Lys Val Ile Lys 
    370                 375                 380                 


Gly Tyr Leu Pro Lys Pro Glu Arg Ile Glu Glu Lys Asp Lys Thr Ile 
385                 390                 395                 400 


Phe Asn Glu Ile Leu Asn Lys Ile Glu Leu Lys Thr Ile Leu Pro Lys 
                405                 410                 415     


Gln Arg Ile Ser Asp Asn Gly Thr Leu Pro Tyr Gln Ile His Glu Val 
            420                 425                 430         


Glu Leu Glu Lys Ile Leu Glu Asn Gln Ser Lys Tyr Tyr Asp Phe Leu 
        435                 440                 445             


Asn Tyr Glu Glu Asn Gly Val Ser Thr Lys Asp Lys Leu Leu Lys Thr 
    450                 455                 460                 


Phe Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Ser Tyr His 
465                 470                 475                 480 


Lys Asp Lys Gly Gly Asn Ser Trp Ile Val Arg Lys Glu Glu Gly Lys 
                485                 490                 495     


Ile Leu Pro Trp Asn Phe Glu Gln Lys Val Asp Ile Glu Lys Ser Ala 
            500                 505                 510         


Glu Glu Phe Ile Lys Arg Met Thr Asn Lys Cys Thr Tyr Leu Asn Gly 
        515                 520                 525             


Glu Asp Val Ile Pro Lys Asp Ser Phe Leu Tyr Ser Glu Tyr Ile Ile 
    530                 535                 540                 


Leu Asn Glu Leu Asn Lys Val Gln Val Asn Asp Glu Phe Leu Asn Glu 
545                 550                 555                 560 


Glu Asn Lys Arg Lys Ile Ile Asp Glu Leu Phe Lys Glu Asn Lys Lys 
                565                 570                 575     


Val Ser Glu Lys Lys Phe Lys Glu Tyr Leu Leu Val Asn Gln Ile Ala 
            580                 585                 590         


Asn Arg Thr Val Glu Leu Lys Gly Ile Lys Asp Ser Phe Asn Ser Asn 
        595                 600                 605             


Tyr Val Ser Tyr Ile Lys Phe Lys Asp Ile Phe Gly Glu Lys Leu Asn 
    610                 615                 620                 


Leu Asp Ile Tyr Lys Glu Ile Ser Glu Lys Ser Ile Leu Trp Lys Cys 
625                 630                 635                 640 


Leu Tyr Gly Asp Asp Lys Lys Ile Phe Glu Lys Lys Ile Lys Asn Glu 
                645                 650                 655     


Tyr Gly Asp Ile Leu Asn Lys Asp Glu Ile Lys Lys Ile Asn Ser Phe 
            660                 665                 670         


Lys Phe Asn Thr Trp Gly Arg Leu Ser Glu Lys Leu Leu Thr Gly Ile 
        675                 680                 685             


Glu Phe Ile Asn Leu Glu Thr Gly Glu Cys Tyr Ser Ser Val Met Glu 
    690                 695                 700                 


Ala Leu Arg Arg Thr Asn Tyr Asn Leu Met Glu Leu Leu Ser Ser Lys 
705                 710                 715                 720 


Phe Thr Leu Gln Glu Ser Ile Asp Asn Glu Asn Lys Glu Met Asn Glu 
                725                 730                 735     


Val Ser Tyr Arg Asp Leu Ile Glu Glu Ser Tyr Val Ser Pro Ser Leu 
            740                 745                 750         


Lys Arg Ala Ile Leu Gln Thr Leu Lys Ile Tyr Glu Glu Ile Lys Lys 
        755                 760                 765             


Ile Thr Gly Arg Val Pro Lys Lys Val Phe Ile Glu Met Ala Arg Gly 
    770                 775                 780                 


Gly Asp Glu Ser Met Lys Asn Lys Lys Ile Pro Ala Arg Gln Glu Gln 
785                 790                 795                 800 


Leu Lys Lys Leu Tyr Asp Ser Cys Gly Asn Asp Ile Ala Asn Phe Ser 
                805                 810                 815     


Ile Asp Ile Lys Glu Met Lys Asn Ser Leu Ser Ser Tyr Asp Asn Asn 
            820                 825                 830         


Ser Leu Arg Gln Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Phe Gly Lys 
        835                 840                 845             


Cys Met Tyr Thr Gly Arg Glu Ile Asp Leu Asp Arg Leu Leu Gln Asn 
    850                 855                 860                 


Asn Asp Thr Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Lys Val Ile 
865                 870                 875                 880 


Lys Asp Asp Ser Phe Asp Asn Leu Val Leu Val Leu Lys Asn Glu Asn 
                885                 890                 895     


Ala Glu Lys Ser Asn Glu Tyr Pro Val Lys Lys Glu Ile Gln Glu Lys 
            900                 905                 910         


Met Lys Ser Phe Trp Arg Phe Leu Lys Glu Lys Asn Phe Ile Ser Asp 
        915                 920                 925             


Glu Lys Tyr Lys Arg Leu Thr Gly Lys Asp Asp Phe Glu Leu Arg Gly 
    930                 935                 940                 


Phe Met Ala Arg Gln Leu Val Asn Val Arg Gln Thr Thr Lys Glu Val 
945                 950                 955                 960 


Gly Lys Ile Leu Gln Gln Ile Glu Pro Glu Ile Lys Ile Val Tyr Ser 
                965                 970                 975     


Lys Ala Glu Ile Ala Ser Ser Phe Arg Glu Met Phe Asp Phe Ile Lys 
            980                 985                 990         


Val Arg Glu Leu Asn Asp Thr His  His Ala Lys Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ile Val  Ala Gly Asn Val Tyr  Asn Thr Lys Phe Thr  Glu Lys Pro 
    1010                 1015                 1020             


Tyr Arg  Tyr Leu Gln Glu Ile  Lys Glu Asn Tyr Asp  Val Lys Lys 
    1025                 1030                 1035             


Ile Tyr  Asn Tyr Asp Ile Lys  Asn Ala Trp Asp Lys  Glu Asn Ser 
    1040                 1045                 1050             


Leu Glu  Ile Val Lys Lys Asn  Met Glu Lys Asn Thr  Val Asn Ile 
    1055                 1060                 1065             


Thr Arg  Phe Ile Lys Glu Glu  Lys Gly Glu Leu Phe  Asn Leu Asn 
    1070                 1075                 1080             


Pro Ile  Lys Lys Gly Glu Thr  Ser Asn Glu Ile Ile  Ser Ile Lys 
    1085                 1090                 1095             


Pro Lys  Leu Tyr Asp Gly Lys  Asp Asn Lys Leu Asn  Glu Lys Tyr 
    1100                 1105                 1110             


Gly Tyr  Tyr Thr Ser Leu Lys  Ala Ala Tyr Phe Ile  Tyr Val Glu 
    1115                 1120                 1125             


His Glu  Lys Lys Asn Lys Lys  Val Lys Thr Phe Glu  Arg Ile Thr 
    1130                 1135                 1140             


Arg Ile  Asp Ser Thr Leu Ile  Lys Asn Glu Lys Asn  Leu Ile Lys 
    1145                 1150                 1155             


Tyr Leu  Val Ser Gln Lys Lys  Leu Leu Asn Pro Lys  Ile Ile Lys 
    1160                 1165                 1170             


Lys Ile  Tyr Lys Glu Gln Thr  Leu Ile Ile Asp Ser  Tyr Pro Tyr 
    1175                 1180                 1185             


Thr Phe  Thr Gly Val Asp Ser  Asn Lys Lys Val Glu  Leu Lys Asn 
    1190                 1195                 1200             


Lys Lys  Gln Leu Tyr Leu Glu  Lys Lys Tyr Glu Gln  Ile Leu Lys 
    1205                 1210                 1215             


Asn Ala  Leu Lys Phe Val Glu  Asp Asn Gln Gly Glu  Thr Glu Glu 
    1220                 1225                 1230             


Asn Tyr  Lys Phe Ile Tyr Leu  Lys Lys Arg Asn Asn  Asn Glu Lys 
    1235                 1240                 1245             


Asn Glu  Thr Ile Asp Ala Val  Lys Glu Arg Tyr Asn  Ile Glu Phe 
    1250                 1255                 1260             


Asn Glu  Met Tyr Asp Lys Phe  Leu Glu Lys Leu Ser  Ser Lys Asp 
    1265                 1270                 1275             


Tyr Lys  Asn Tyr Ile Asn Asn  Lys Leu Tyr Thr Asn  Phe Leu Asn 
    1280                 1285                 1290             


Ser Lys  Glu Lys Phe Lys Lys  Leu Lys Leu Trp Glu  Lys Ser Leu 
    1295                 1300                 1305             


Ile Leu  Arg Glu Phe Leu Lys  Ile Phe Asn Lys Asn  Thr Tyr Gly 
    1310                 1315                 1320             


Lys Tyr  Glu Ile Lys Asp Ser  Gln Thr Lys Glu Lys  Leu Phe Ser 
    1325                 1330                 1335             


Phe Pro  Glu Asp Thr Gly Arg  Ile Arg Leu Gly Gln  Ser Ser Leu 
    1340                 1345                 1350             


Gly Asn  Asn Lys Glu Leu Leu  Glu Glu Ser Val Thr  Gly Leu Phe 
    1355                 1360                 1365             


Val Lys  Lys Ile Lys Leu 
    1370                 


<210>  1057
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1057

Met Ser Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Phe Glu Glu Met Ser Lys Leu Asp Ser 
                85                  90                  95      


Gly Phe Phe His Arg Leu Asp Asp Phe Phe Leu Ile Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Thr Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln 
        195                 200                 205             


Ile Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Glu Leu Glu Glu Lys Ala Pro Leu Gln Phe Pro Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Val Leu Gly Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Val Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu 
                325                 330                 335     


Lys Gln Phe Ile Lys Thr Asn Leu Pro Glu Lys Tyr Val Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Thr Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Lys Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Lys Lys Asp Ile Ile His Tyr Leu His Thr Val Asp Gly Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Lys Phe Met Asp 
        595                 600                 605             


Asp Ser Lys Asn Glu Ala Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 


Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Cys Asp 
            660                 665                 670         


Lys Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Glu 
        675                 680                 685             


Ile Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 


Lys Lys Ile Ile Gln Lys Ser Gln Val Val Gly Glu Thr Asp Asp Val 
705                 710                 715                 720 


Lys Gln Val Val Arg Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Asp 
            740                 745                 750         


His Ala Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ser Leu Lys Asn Leu Ala Ser Gly Leu Asp Ser Asn Ile Leu Lys 
785                 790                 795                 800 


Glu Asn Pro Thr Asp Asn Ile Gln Leu Lys Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp 
            820                 825                 830         


Ile Asn Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val Val Gln 
865                 870                 875                 880 


Lys Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Glu Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asn 
            900                 905                 910         


Glu Arg Asp Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Tyr Asn Thr 
    930                 935                 940                 


Glu Val Asn Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr 
945                 950                 955                 960 


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Arg Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Ala Val Val Ala Lys Ala Ile  Leu Lys Lys Tyr Pro  Lys Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Glu  Tyr Gln Lys Tyr Asp  Leu Lys Arg 
    1010                 1015                 1020             


Tyr Ile  Ser Arg Ser Arg Asn  Pro Lys Glu Val Glu  Lys Ala Thr 
    1025                 1030                 1035             


Glu Lys  Tyr Phe Phe Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Glu 
    1040                 1045                 1050             


Glu Val  His Tyr Ala Asp Gly  Thr Ile Val Lys Arg  Glu Asn Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Thr Glu Lys 
    1070                 1075                 1080             


Asp Phe  Ala Thr Ile Lys Lys  Ile Leu Ser Tyr Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Thr Glu Glu  Gln Thr Val Gly Gln  Asn Gly Gly 
    1100                 1105                 1110             


Leu Phe  Asp Asn Asn Ile Val  Ser Lys Glu Lys Val  Val Asp Ala 
    1115                 1120                 1125             


Ser Lys  Leu Ile Pro Ile Lys  Ser Gly Leu Ser Pro  Lys Lys Tyr 
    1130                 1135                 1140             


Gly Gly  Tyr Ala Arg Pro Thr  Ile Ala Tyr Ser Val  Leu Val Thr 
    1145                 1150                 1155             


Tyr Lys  Gln Asn Asn Lys Ile  Lys Asn Thr Met Val  Gly Ile Pro 
    1160                 1165                 1170             


Val Leu  Thr Lys Leu Glu Phe  Glu Arg Asp Pro Asn  Leu Tyr Leu 
    1175                 1180                 1185             


Arg Asn  Leu Gly Ile Asn Asn  Val Ser His Val Ile  Lys Leu Val 
    1190                 1195                 1200             


Lys Tyr  Thr Val Leu Glu Phe  Lys His Lys Tyr Gly  Thr Tyr Arg 
    1205                 1210                 1215             


Arg Phe  Ile Val Ser Asn Gln  Glu Leu Lys Arg Ala  Asn Gln Ile 
    1220                 1225                 1230             


Phe Val  Thr Thr Lys Gln Met  Lys Leu Ile Ser Gln  Tyr Thr Thr 
    1235                 1240                 1245             


Leu Asp  Lys Asn Glu His Asp  Ser Leu Leu Lys Asn  Asn Gln Gln 
    1250                 1255                 1260             


Leu Ile  Gln Ser Ile Trp Asn  Gln Phe Leu Glu Phe  Met Leu Ala 
    1265                 1270                 1275             


Thr Asp  Leu Val Asn Lys Lys  Gln Tyr Asp Glu Leu  Ile Leu Thr 
    1280                 1285                 1290             


Asn Asn  Gln Thr Ile Glu Glu  Cys Arg Asn Ile Met  Asn Arg Val 
    1295                 1300                 1305             


Ile Thr  Thr Leu Ser Phe Thr  Asn Ile Asn Ala Gly  Gln Lys Lys 
    1310                 1315                 1320             


Ile Ile  Phe Asp Asp Thr Leu  Ser Leu Pro Ile Lys  Arg Trp Gln 
    1325                 1330                 1335             


Phe Thr  Asp Ser Asp Lys Met  Val Lys Lys Ile Ile  Glu Ser Thr 
    1340                 1345                 1350             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1355                 1360                 1365             


Leu Ser  Lys Leu Gly Glu Asp  
    1370                 1375 


<210>  1058
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1058

Met Lys Glu Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile Gly 
1               5                   10                  15      


Trp Ala Val Thr Asp Ser Gln Phe Lys Leu Met Arg Ile Lys Gly Lys 
            20                  25                  30          


Thr Ala Ile Gly Val Arg Leu Phe Glu Glu Gly Lys Thr Ala Ala Glu 
        35                  40                  45              


Arg Arg Thr Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Lys Trp 
    50                  55                  60                  


Arg Leu His Tyr Leu Asp Glu Ile Phe Ala Pro His Leu Gln Glu Val 
65                  70                  75                  80  


Asp Glu Asn Phe Leu Arg Arg Leu Lys Gln Ser Asn Ile His Pro Glu 
                85                  90                  95      


Asp Pro Ala Lys Asn Gln Ala Phe Ile Gly Lys Leu Leu Phe Pro Asp 
            100                 105                 110         


Leu Leu Lys Lys Asn Glu Arg Gly Tyr Pro Thr Leu Ile Lys Met Arg 
        115                 120                 125             


Asp Glu Leu Pro Val Glu Gln Arg Ala His Tyr Pro Val Thr Asn Ile 
    130                 135                 140                 


Tyr Lys Leu Arg Glu Ala Met Ile Asn Glu Asp Arg Gln Phe Asp Leu 
145                 150                 155                 160 


Arg Glu Val Tyr Leu Ala Val His His Ile Val Lys Tyr Arg Gly His 
                165                 170                 175     


Phe Leu Asn Asn Ala Ser Val Asp Lys Phe Lys Val Gly Arg Ile Asp 
            180                 185                 190         


Phe Asp Lys Ser Phe Asn Val Leu Asn Glu Ala Tyr Glu Glu Leu Gln 
        195                 200                 205             


Asn Gly Glu Gly Ser Phe Thr Ile Glu Pro Ser Lys Val Glu Lys Ile 
    210                 215                 220                 


Gly Gln Leu Leu Leu Asp Thr Lys Met Arg Lys Leu Asp Arg Gln Lys 
225                 230                 235                 240 


Ala Val Ala Lys Leu Leu Glu Val Lys Val Ala Asp Lys Glu Glu Thr 
                245                 250                 255     


Lys Arg Asn Lys Gln Ile Ala Thr Ala Met Ser Lys Leu Val Leu Gly 
            260                 265                 270         


Tyr Lys Ala Asp Phe Ala Thr Val Ala Met Val Asn Gly Asn Glu Trp 
        275                 280                 285             


Lys Ile Asp Leu Ser Ser Glu Thr Ser Glu Asp Glu Ile Glu Lys Phe 
    290                 295                 300                 


Arg Glu Glu Leu Ser Asp Ala Gln Asn Asp Ile Leu Thr Glu Ile Thr 
305                 310                 315                 320 


Ser Leu Phe Ser Gln Ile Met Leu Asn Glu Ile Val Pro Asn Gly Met 
                325                 330                 335     


Ser Ile Ser Glu Ser Met Met Asp Arg Tyr Trp Thr His Glu Arg Gln 
            340                 345                 350         


Leu Ala Glu Val Lys Glu Tyr Leu Ala Thr Gln Pro Ala Ser Ala Arg 
        355                 360                 365             


Lys Glu Phe Asp Gln Val Tyr Asn Lys Tyr Ile Gly Gln Ala Pro Lys 
    370                 375                 380                 


Glu Lys Gly Phe Asp Leu Glu Lys Gly Leu Lys Lys Ile Leu Ser Lys 
385                 390                 395                 400 


Lys Glu Asn Trp Lys Glu Ile Asp Glu Leu Leu Lys Ala Gly Asp Phe 
                405                 410                 415     


Leu Pro Lys Gln Arg Ile Ser Ala Asn Gly Val Ile Pro His Gln Met 
            420                 425                 430         


His Gln Gln Glu Leu Asp Arg Ile Ile Glu Lys Gln Ala Lys Tyr Tyr 
        435                 440                 445             


Pro Trp Leu Ala Thr Glu Asn Pro Ala Thr Gly Glu Arg Asp Arg His 
    450                 455                 460                 


Gln Ala Lys Tyr Glu Leu Asp Gln Leu Val Ser Phe Arg Ile Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Val Thr Pro Glu Val Gln Lys Ala Thr Ser Gly 
                485                 490                 495     


Ala Lys Phe Ala Trp Ala Lys Arg Lys Glu Asp Gly Glu Ile Thr Pro 
            500                 505                 510         


Trp Asn Leu Trp Asp Lys Ile Asp Arg Ala Glu Ser Ala Glu Ala Phe 
        515                 520                 525             


Ile Lys Arg Met Thr Val Lys Asp Thr Tyr Leu Leu Asn Glu Asp Val 
    530                 535                 540                 


Leu Pro Ala Asn Ser Leu Leu Tyr Gln Lys Tyr Asn Val Leu Asn Glu 
545                 550                 555                 560 


Leu Asn Asn Val Arg Val Asn Gly Arg Arg Leu Ser Val Gly Ile Lys 
                565                 570                 575     


Gln Asp Ile Tyr Thr Glu Leu Phe Lys Lys Lys Lys Thr Val Lys Ala 
            580                 585                 590         


Ser Asp Val Ala Ser Leu Val Met Ala Lys Thr Arg Gly Val Asn Lys 
        595                 600                 605             


Pro Ser Val Glu Gly Leu Ser Asp Pro Lys Lys Phe Asn Ser Asn Leu 
    610                 615                 620                 


Ala Thr Tyr Leu Asp Leu Lys Ser Ile Val Gly Asp Lys Val Asp Asp 
625                 630                 635                 640 


Asn Arg Tyr Gln Met Asp Leu Glu Asn Ile Ile Glu Trp Arg Ser Val 
                645                 650                 655     


Phe Glu Asp Gly Glu Ile Phe Ala Asp Lys Leu Thr Glu Val Glu Trp 
            660                 665                 670         


Leu Thr Asp Glu Gln Arg Ser Ala Leu Val Lys Lys Arg Tyr Lys Gly 
        675                 680                 685             


Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gly Ile Val Asp Glu Asn 
    690                 695                 700                 


Gly Gln Arg Ile Ile Asp Leu Met Trp Asn Thr Asp Gln Asn Phe Met 
705                 710                 715                 720 


Gln Ile Val Asn Gln Pro Val Phe Lys Glu Gln Ile Asp Gln Leu Asn 
                725                 730                 735     


Gln Lys Ala Ile Thr Asn Asp Gly Met Thr Leu Arg Glu Arg Val Glu 
            740                 745                 750         


Ser Val Leu Asp Asp Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile 
        755                 760                 765             


Trp Gln Val Val Arg Val Val Glu Asp Ile Val Lys Ala Val Gly Asn 
    770                 775                 780                 


Ala Pro Lys Ser Ile Ser Ile Glu Phe Ala Arg Asn Glu Gly Asn Lys 
785                 790                 795                 800 


Gly Glu Ile Thr Arg Ser Arg Arg Thr Gln Leu Gln Lys Leu Phe Glu 
                805                 810                 815     


Asp Gln Ala His Glu Leu Val Lys Asp Thr Ser Leu Thr Glu Glu Leu 
            820                 825                 830         


Glu Lys Val Pro Asp Leu Ser Asp Arg Tyr Tyr Phe Tyr Phe Thr Gln 
        835                 840                 845             


Gly Gly Lys Asp Met Tyr Thr Gly Asp Pro Ile Asn Phe Asp Glu Ile 
    850                 855                 860                 


Ser Thr Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe Val Lys 
865                 870                 875                 880 


Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Arg Lys Glu Asn Asn 
                885                 890                 895     


Lys Lys Ser Asp Gln Val Pro Ala Lys Leu Tyr Ala Ala Lys Met Lys 
            900                 905                 910         


Pro Tyr Trp Asn Gln Leu Leu Lys Gln Gly Leu Ile Thr Gln Arg Lys 
        915                 920                 925             


Phe Glu Asn Leu Thr Met Asp Val Asp Gln Thr Ile Lys Tyr Arg Ser 
    930                 935                 940                 


Leu Gly Phe Val Lys Arg Gln Leu Val Glu Thr Arg Gln Val Ile Lys 
945                 950                 955                 960 


Leu Thr Ala Asn Ile Leu Gly Ser Met Tyr Gln Glu Ala Gly Thr Asp 
                965                 970                 975     


Ile Ile Glu Thr Arg Ala Gly Leu Thr Lys Gln Leu Arg Glu Glu Phe 
            980                 985                 990         


Asp Leu Pro Lys Val Arg Glu Val  Asn Asp Tyr His His  Ala Val Asp 
        995                 1000                 1005             


Ala Tyr  Leu Thr Thr Phe Ala  Gly Gln Tyr Leu Asn  Arg Arg Tyr 
    1010                 1015                 1020             


Pro Lys  Leu Arg Ser Phe Phe  Val Tyr Gly Glu Tyr  Met Lys Phe 
    1025                 1030                 1035             


Lys His  Gly Ser Asp Leu Lys  Leu Arg Asn Phe Asn  Phe Phe His 
    1040                 1045                 1050             


Glu Leu  Met Glu Gly Asp Lys  Ser Gln Gly Lys Val  Val Asp Gln 
    1055                 1060                 1065             


Gln Thr  Gly Glu Leu Ile Thr  Thr Arg Asp Glu Val  Ala Asp Tyr 
    1070                 1075                 1080             


Phe Asp  Trp Val Gln Asp Leu  Lys Leu Met Leu Val  Thr Lys Glu 
    1085                 1090                 1095             


Leu Lys  Arg Ser Asn Gly Lys  Leu Phe Asp Ala Thr  Ile Gln Asn 
    1100                 1105                 1110             


Ala Asn  Tyr Thr Lys Leu Asn  Glu Arg Lys Lys Val  Phe Val Pro 
    1115                 1120                 1125             


Leu Lys  Lys Glu Leu Asp Lys  Thr Leu Tyr Gly Ser  Tyr Ser Gly 
    1130                 1135                 1140             


Ile Gln  Ser Ser Tyr Met Thr  Ile Val Lys Lys Phe  Asp Lys Lys 
    1145                 1150                 1155             


Gly Ser  Thr Tyr Ala Val Gln  Arg Ile Pro Val Met  Tyr Ser Lys 
    1160                 1165                 1170             


Leu Leu  Glu Leu Glu Lys Glu  Gln Ser Lys Leu Asn  Arg Leu Val 
    1175                 1180                 1185             


Gln Ser  Ile Val Thr Ser Asp  Pro Gln Ala Pro Asn  Asn Phe Glu 
    1190                 1195                 1200             


Val Leu  Met Pro Cys Val Leu  Ser Gly Gln Leu Val  Ile Asp Asn 
    1205                 1210                 1215             


Gly Arg  Lys Phe Thr Leu Thr  Ser Tyr Arg Tyr Gln  Cys Ser Thr 
    1220                 1225                 1230             


Ala Gln  Leu Phe Leu Pro Lys  Lys Asp Leu Lys Tyr  Ile Gln Asn 
    1235                 1240                 1245             


Asn Phe  Lys Glu Leu Lys Lys  Thr Pro Asp Lys Gln  Asp Lys Ile 
    1250                 1255                 1260             


Phe Glu  Thr Ile Tyr Asp Asp  Ile Leu Lys Gln Val  Asp Lys Tyr 
    1265                 1270                 1275             


Phe Ser  Leu Tyr Asp Ile Asn  Lys Phe Arg Ser Lys  Leu His Asp 
    1280                 1285                 1290             


Gly Lys  Asp Lys Phe Leu Ala  Leu Glu Asn Phe Gly  Glu Lys Glu 
    1295                 1300                 1305             


Ser Lys  Asp Lys Val Ile Thr  Lys Ile Leu Arg Gly  Leu His Ala 
    1310                 1315                 1320             


Asn Ala  Glu Arg Thr Ser Leu  Lys Glu Ile Gly Leu  Ser Thr Pro 
    1325                 1330                 1335             


Leu Gly  Gln Phe Gln Ser Gly  Glu Gly Gly Ile Thr  Leu Ser Asp 
    1340                 1345                 1350             


Asn Thr  Lys Ile Ile Tyr Gln  Ser Pro Thr Gly Leu  Phe Glu Arg 
    1355                 1360                 1365             


His Val  Tyr Leu Lys Asp Leu  
    1370                 1375 


<210>  1059
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1059

Met Arg Lys Asn Gln Glu Pro Tyr Asn Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Ser Ser Ile Gly Trp Ser Ile Met Asn Asp Asn Phe Asp Leu Met Arg 
            20                  25                  30          


Val Lys Gly Lys Lys Gly Ile Gly Val Arg Leu Tyr Asn Glu Gly Gln 
        35                  40                  45              


Ser Ala Ala Glu Arg Arg Met His Arg Thr Ala Arg Arg Arg Tyr Gly 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Arg Leu Leu Glu Asp Phe Phe Asp Glu His 
65                  70                  75                  80  


Met Ala Glu Val Asp Asp Thr Phe Phe Ala Arg Leu Lys Asp Ser Asn 
                85                  90                  95      


Ile Ser Pro Lys Asp Asp Lys Lys Tyr Arg Lys Ser Leu Leu Phe Pro 
            100                 105                 110         


Lys Ser Lys Gly Val Thr Tyr Gln Asp Asp Gly Glu Phe Tyr Lys Lys 
        115                 120                 125             


Tyr Pro Thr Met Tyr His Leu Arg Tyr Ala Leu Met Thr Glu His Arg 
    130                 135                 140                 


Lys Phe Asp Leu Arg Glu Ile Tyr Leu Ala Phe His His Met Val Lys 
145                 150                 155                 160 


Tyr Arg Gly Asn Phe Leu Tyr Asp Thr Ser Val Asp Ser Phe Glu Ala 
                165                 170                 175     


Lys Asn Leu Asp Ile Lys Gly Lys Phe Asp Glu Ile Asn Asp Leu Leu 
            180                 185                 190         


Ser Ser Tyr Thr Asp Phe Tyr Val Asp Asn Ser Asn Ala Ala Leu Val 
        195                 200                 205             


Glu Ser Ile Leu Leu Glu Lys Asn Thr Thr Arg Lys Asp Lys Ser Lys 
    210                 215                 220                 


Lys Ile Ala Lys Leu Leu His Val Glu Asp Lys Glu Lys Gly Lys Asn 
225                 230                 235                 240 


Lys Lys Ala Lys Asp Leu Ala Thr Gln Ile Ser Asn Ala Val Leu Gly 
                245                 250                 255     


Leu Lys Cys Asn Phe His Leu Ile Phe Glu Leu Gln Gln Lys Tyr Ser 
            260                 265                 270         


Phe Asp Leu Cys Ser Glu Lys Thr Glu Glu Asn Ile Ala Lys Leu Ser 
        275                 280                 285             


Glu Val Leu Asp Glu Asn Gln Lys Met Leu Leu Met Ile Leu Lys Glu 
    290                 295                 300                 


Val Gln Asp Gln Val Met Leu Asn Ala Phe Val Pro Ser Gly Met Ser 
305                 310                 315                 320 


Leu Ser Glu Ala Met Met Gln Lys Tyr Asp Asp Tyr Gly Glu Gln Leu 
                325                 330                 335     


Lys Ile Tyr His Glu Leu Glu His Ser Val Ser Asp Asp Ser Ala Ala 
            340                 345                 350         


Lys Leu Arg Gly Ala Tyr Arg Asp Tyr Asn Asp Asn Ile Ile Lys Arg 
        355                 360                 365             


Ile Asp Gly Asp Asn Lys Lys Asp Thr Phe Tyr Lys Arg Val Lys Asn 
    370                 375                 380                 


Ile Leu Thr Lys Ile Ser Lys Glu Tyr Val Asp Gln Asp Val Leu Glu 
385                 390                 395                 400 


Ser Cys Gly Lys Leu Lys Lys Leu Ile Asp Glu Asn Lys Leu Phe Ile 
                405                 410                 415     


Arg Gln Arg Thr Ala Ala Asn Gly Val Leu Pro His Gln Leu His Gln 
            420                 425                 430         


Ile Glu Met Arg Lys Ile Ile Asp Asn Gln Lys Glu Tyr Tyr Pro Trp 
        435                 440                 445             


Leu Ala Glu Pro Asn Pro Asn Glu Lys Arg Arg Val Tyr Ser Lys Tyr 
    450                 455                 460                 


Lys Val Glu Glu Leu Ile Ala Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
465                 470                 475                 480 


Leu Val Asp Pro Asn Asn Ala Asp Lys Asn Lys Glu Ala Arg Phe Ser 
                485                 490                 495     


Trp Met Val Arg Lys Lys Asp Gly Glu Ile Thr Pro Trp Asn Phe Tyr 
            500                 505                 510         


Asp Lys Val Asp Trp Ala Glu Ser Ala Asn Asn Phe Ile Glu Arg Met 
        515                 520                 525             


Lys Ser Lys Asp Thr Tyr Leu Leu Gly Glu Asp Val Val Pro Lys Glu 
    530                 535                 540                 


Ser Met Leu Tyr Gln Lys Tyr Glu Val Leu Asn Glu Leu Asn Asn Leu 
545                 550                 555                 560 


Arg Ile Asn Asp Ser Gly Leu Ser Asp Ser Phe Glu Asp Val Lys Leu 
                565                 570                 575     


Lys Gln Ala Ile Tyr Asn Asp Leu Phe Lys Lys Gln Lys Ile Val Lys 
            580                 585                 590         


Ile Thr Asp Leu Gln Asn Tyr Leu Val Gln Asn His Lys Tyr Leu Val 
        595                 600                 605             


Lys Pro Lys Ile Ser Gly Leu Ala Asp Glu Asn Arg Phe Leu Ser Ser 
    610                 615                 620                 


Leu Ser Thr Tyr Ser Asp Leu Lys Thr Ile Phe Gly Asp Lys Val Asp 
625                 630                 635                 640 


Asp Arg Thr Tyr Phe Asn Asp Phe Glu Lys Met Val Glu Tyr Ser Thr 
                645                 650                 655     


Val Phe Glu Asp Gly His Asp Tyr Asn Gln Lys Leu Asp Glu Tyr Thr 
            660                 665                 670         


Trp Leu Thr Lys Glu Glu Lys Tyr Lys Ile Gly Lys Lys Arg Tyr Arg 
        675                 680                 685             


Gly Trp Gly Lys Leu Ser Lys Lys Leu Leu Thr Gly Leu Arg Asp Lys 
    690                 695                 700                 


Asn Asn His Thr Ile Met Asp Asn Leu Trp Glu Thr Asn Arg Asn Phe 
705                 710                 715                 720 


Met Gln Ile Gln Thr Ala Asp Glu Phe Ser Lys Gln Ile Ala Glu Glu 
                725                 730                 735     


Asn Glu Arg His Leu Lys Gly Ser Val Ser Asp Ala Ile Asn Asp Met 
            740                 745                 750         


Tyr Thr Ser Pro Ala Asn Lys Lys Ala Ile Arg Gln Val Leu Arg Val 
        755                 760                 765             


Val Asp Asp Ile Gln Lys Ala Met Gly Tyr Ala Pro Ser Ser Ile Ser 
    770                 775                 780                 


Leu Glu Phe Ala Arg Glu Asp Gly Pro Ser Val Arg Thr Val Ser Arg 
785                 790                 795                 800 


Ala Asn Arg Met Lys Ser Ile Tyr Glu Lys Tyr Ala Ser Glu Val Ser 
                805                 810                 815     


Glu Glu Val Met Lys Asp Leu Asp Gly Val Ile Lys Asp Lys Lys Gly 
            820                 825                 830         


Leu Asn Asp Arg Leu Tyr Leu Tyr Phe Glu Gln Gln Gly Lys Asp Met 
        835                 840                 845             


Tyr Ser Gly His Pro Leu Asp Phe Asp Lys Val Ile Ser Gly Gln Glu 
    850                 855                 860                 


Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Val Ile Lys Asp Asp Ser 
865                 870                 875                 880 


Leu Asp Asn Arg Val Leu Thr Thr Lys Ala Leu Asn Asn Asp Val Lys 
                885                 890                 895     


Ser Lys Gly Val Pro Cys Arg Met Phe Asn Gly Met His Ser Phe Trp 
            900                 905                 910         


Lys Asn Leu Tyr Asp Lys Gly Phe Ile Ser Arg Arg Lys Phe Asn Asn 
        915                 920                 925             


Leu Thr Thr Asn Pro Glu Asn Ile Asp Lys Tyr Lys Met Lys Gly Phe 
    930                 935                 940                 


Val Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys Leu Val Ala 
945                 950                 955                 960 


Asn Val Leu Asn Asp Lys Tyr Gln Asn Asp Asp Val Asp Ile Ile Glu 
                965                 970                 975     


Val Arg Ala Glu Leu Thr His Asp Val Arg Lys His Phe Lys Phe Tyr 
            980                 985                 990         


Lys Asn Arg Asn Val Asn Asp Tyr  His His Ala Phe Asp  Ala Tyr Leu 
        995                 1000                 1005             


Thr Ser  Phe Ile Gly His Tyr  Leu Phe Lys Lys Tyr  Pro Asn Leu 
    1010                 1015                 1020             


Arg Pro  Leu Phe Asp Tyr Asn  Asp Phe Met Lys Val  Ser Asp Asn 
    1025                 1030                 1035             


Val Phe  Lys Gln Leu Arg Gly  Asn Asn Phe Leu Gly  Glu Phe Leu 
    1040                 1045                 1050             


Asn Lys  Thr Gly Asp Ile Ile  Ser Thr Asp Gly Asn  Phe Val Leu 
    1055                 1060                 1065             


Asn Lys  Glu Glu Met Ile Asn  Lys Leu Asn Lys Ala  Tyr Ala Phe 
    1070                 1075                 1080             


Lys Lys  Ile Leu Val Thr Lys  Glu Val Gly Gln Arg  Thr Gly Ala 
    1085                 1090                 1095             


Met Phe  Asn Glu Thr Arg Tyr  Pro Ala Pro Asn Ser  Arg Lys Ala 
    1100                 1105                 1110             


Ala Leu  Lys Tyr Arg Arg Met  Pro Lys Pro Asp Ser  Leu Ile Ser 
    1115                 1120                 1125             


Val Lys  Asp Tyr Lys Asn Thr  Asp Ile Tyr Gly Gly  Tyr Ser Gly 
    1130                 1135                 1140             


Lys Asn  Asp Ala Tyr Met Val  Ile Val Asp Met Gly  Lys Gln Tyr 
    1145                 1150                 1155             


Met Val  Val Gly Val Pro Val  Arg Tyr Thr Glu Lys  Leu Asp Lys 
    1160                 1165                 1170             


Leu Arg  Ile Lys His Ser Glu  Leu Tyr Arg Asp Glu  Leu Arg Lys 
    1175                 1180                 1185             


Val Leu  Ser Arg Asp Lys Thr  Leu Leu Asp Ser Lys  Gly Asn Val 
    1190                 1195                 1200             


Lys Arg  Phe Asp Ile Val Leu  Asp Arg Val Leu Tyr  Gly Gln Leu 
    1205                 1210                 1215             


Ile Glu  Asp Gly Asn Glu Leu  Phe Thr Leu Gly Ser  Ser Lys Tyr 
    1220                 1225                 1230             


Lys Arg  Asn Phe Arg Gln Leu  Phe Leu Asp Lys Gln  Cys Ile Glu 
    1235                 1240                 1245             


Ile Leu  Asp Ser Ser Ala Thr  Pro Gln Pro Thr Asp  Glu Glu Leu 
    1250                 1255                 1260             


Ile Trp  Val Tyr Asp Gln Ile  Leu Asp Lys Val Asp  Lys Tyr Phe 
    1265                 1270                 1275             


Glu Leu  Tyr Asp Ile Asn Arg  Gln Arg Glu Thr Leu  Arg Lys Gly 
    1280                 1285                 1290             


Arg Ala  Ala Phe Cys Ser Leu  Pro Asn Ile Ser Met  Lys Lys Asp 
    1295                 1300                 1305             


Asp Val  Thr Lys Lys Lys Ile  Leu Asn Glu Ile Leu  Val Ala Leu 
    1310                 1315                 1320             


His Ala  Asn Glu Ser Glu Ser  Asn Cys Glu Lys Ile  Gly Lys Lys 
    1325                 1330                 1335             


Val Phe  Gly Arg Leu Gln Val  Pro Gly Gly Ile Lys  Leu Ser Lys 
    1340                 1345                 1350             


Glu Ala  Lys Leu Ile Tyr Gln  Ser Pro Thr Gly Leu  Phe Glu Arg 
    1355                 1360                 1365             


Val Val  Tyr Leu Lys Asp Leu  
    1370                 1375 


<210>  1060
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1060

Met Glu Asn Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Phe Ala Val Val Asp Glu Asn Asn Arg Val Met Arg Val Lys 
            20                  25                  30          


Gly Lys Asn Ala Ile Gly Val Arg Leu Phe Ser Glu Gly Gln Pro Ala 
        35                  40                  45              


Ala Asp Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Arg Trp Arg Leu Arg Leu Leu Arg Glu Ile Phe Glu Pro Tyr Ile Asn 
65                  70                  75                  80  


Pro Val Asp Glu Ala Phe Phe Met Arg Leu Lys Glu Ser Asn Leu Ser 
                85                  90                  95      


Pro Lys Asp Ala Asn Lys His Tyr Ser Gly Asp Ile Leu Phe Asn Asn 
            100                 105                 110         


Gln Thr Asp Gln Asp Phe Tyr Glu Lys Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg His Ala Leu Met Thr Glu His Arg Lys Phe Asp Val Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Val Lys Phe Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Ala Ala Ser Ala Ser Asn Phe Thr Val Gly Lys Leu Asn Leu Glu Gly 
                165                 170                 175     


Lys Phe Asp Ala Leu Asn Glu Ile Tyr Gln Arg Val Leu Thr Asp Asp 
            180                 185                 190         


Pro Phe Ala Leu Arg Thr Asp Asn Leu Asp His Ile Lys Glu Ile Leu 
        195                 200                 205             


Ile Asp Asn Lys Arg Ser Gln Ala Asp Arg Gln Arg Thr Leu Thr Gly 
    210                 215                 220                 


Asp Val Tyr Gln Ser Ser Glu Glu Lys Glu Ile Glu Lys Arg Asn Lys 
225                 230                 235                 240 


Ala Ile Ala Thr Glu Ile Leu Lys Ala Ala Val Gly Asn Lys Ala Lys 
                245                 250                 255     


Leu Asn Val Ile Thr Asn Val Glu Val Asp Lys Glu Ala Ala Lys Asp 
            260                 265                 270         


Trp Ser Leu Thr Phe Asp Ser Glu Thr Ile Asp Asp Glu Leu Ala Lys 
        275                 280                 285             


Val Asp Gly Gln Met Thr Asp Asp Gly Arg Glu Ile Ile Glu Ile Leu 
    290                 295                 300                 


Arg Ser Leu Tyr Ser Gly Ile Thr Leu Ala Ala Ile Val Pro Glu Asn 
305                 310                 315                 320 


His Thr Leu Ser Gln Ser Met Val Glu Lys Tyr Asn Ser His Lys Ala 
                325                 330                 335     


His Leu Lys Leu Leu Lys Asp Leu Ile Asn Asn Met Thr Asp Thr Lys 
            340                 345                 350         


Lys Ala Lys Asn Leu Arg Ala Ala Tyr Asp Gly Tyr Ile Asp Gly Val 
        355                 360                 365             


Lys Gly Lys Val Leu Thr Arg Asp Asp Phe Tyr Lys Gln Ile Gln Gly 
    370                 375                 380                 


Asn Leu Asp Asp Ser Ala Glu Ala Gln Lys Ile Gln Asp Val Met Asp 
385                 390                 395                 400 


Gln Asp Glu Phe Met Pro Lys Gln Arg Thr Lys Ala Asn Gly Ser Ile 
                405                 410                 415     


Pro His Gln Leu Gln Gln Gln Glu Leu Asp Gln Ile Val Glu Asn Gln 
            420                 425                 430         


Lys Glu Tyr Tyr Pro Trp Leu Ala Glu Glu Asn Pro Asn Glu Thr Arg 
        435                 440                 445             


Arg Gly Val Ala Lys Tyr Lys Leu Asp Glu Leu Val Thr Phe Arg Val 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Met Ile Thr Ala Glu Asp Gln Glu Lys Gln 
465                 470                 475                 480 


Ser Gly Ala Ser Phe Ala Trp Met Lys Arg Lys Glu Pro Gly Asn Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Met Ala Ser Ala Asn 
            500                 505                 510         


Gln Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu Gly Glu 
        515                 520                 525             


Asp Val Leu Pro Ala Gln Ser Leu Leu Tyr Gln Arg Phe Glu Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Lys Ile Arg Ile Asp Arg Lys Pro Ile Ser Ile Glu 
545                 550                 555                 560 


Leu Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Lys Phe Lys Asn Val 
                565                 570                 575     


Thr Ile Lys His Leu Gln Glu Tyr Leu Val Ala Thr Gly Gly Tyr Asp 
            580                 585                 590         


Lys Arg Pro Arg Ile Asp Gly Leu Ala Asp Glu Lys Arg Phe Asn Ser 
        595                 600                 605             


Gly Leu Ser Ala Tyr His Asp Leu His Gly Ile Leu Gly Ala Glu Val 
    610                 615                 620                 


Val Asp Ala Asn Asp Lys Gln Asp Asp Leu Glu Lys Ile Ile Glu Trp 
625                 630                 635                 640 


Ser Thr Ile Phe Glu Asp Lys Lys Ile Tyr Arg Ala Lys Leu Asp Glu 
                645                 650                 655     


Leu Thr Trp Leu Thr Asp Ser Gln Lys Glu Lys Leu Ala Thr Lys Arg 
            660                 665                 670         


Tyr Gln Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Ser Gly Leu Arg 
        675                 680                 685             


Asn Ser Asp His Arg Ser Ile Leu Asp Ile Leu Trp Ile Thr Asn Glu 
    690                 695                 700                 


Asn Phe Met Gln Ile Gln Ala Asp Pro Asp Phe Ala Glu Leu Val Lys 
705                 710                 715                 720 


Asn Ala Asn Gln Gly Met Leu Gly Lys Asn Asn Ser Gln Asp Val Ile 
                725                 730                 735     


Asn Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Ile 
            740                 745                 750         


Leu Leu Val Val His Asp Ile Gln Asn Ala Met His Gly Gln Ala Pro 
        755                 760                 765             


Ala Lys Ile His Val Glu Phe Ala Arg Gly Glu Glu Arg Asn Pro Arg 
    770                 775                 780                 


Arg Ser Ile Gln Arg Gln His Gln Ile Glu Ala Ala Tyr Asp Lys Val 
785                 790                 795                 800 


Thr Ser Asp Leu Leu Val Asp Ala Gly Val Arg Gln Glu Leu Lys Asp 
                805                 810                 815     


Ala Ile Asn Asp Lys Arg Asp Phe Lys Asp Arg Leu Phe Leu Tyr Phe 
            820                 825                 830         


Met Gln Gly Gly Arg Asp Ile Tyr Asn Gly Glu Pro Ile Asn Ile Asp 
        835                 840                 845             


Arg Leu Ser Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Phe Val 
    850                 855                 860                 


Lys Asp Asp Ser Leu Asn Asn Arg Val Leu Thr Asp Ala Glu Gly Asn 
865                 870                 875                 880 


Arg Arg Lys Ser Asp Ala Val Pro Ser Glu Leu Phe Gly Ala Lys Met 
                885                 890                 895     


Arg Ser Thr Trp Glu Leu Met Arg Glu Gln Gly Leu Ile Ser Lys Ala 
            900                 905                 910         


Lys Tyr Arg Asn Leu Thr Met Asn Pro Glu Asn Ile Asn Ala His Thr 
        915                 920                 925             


Glu Asn Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val Ile 
    930                 935                 940                 


Lys Leu Ala Val Asn Ile Leu Ala Asp Glu Tyr Thr Glu Asp Thr Gln 
945                 950                 955                 960 


Ile Val Ser Val Lys Ala Asp Leu Ser His Gln Met Arg Glu Asp Phe 
                965                 970                 975     


Glu Leu Leu Lys Asn Arg Asp Val Asn Asp Tyr His His Ala Phe Asp 
            980                 985                 990         


Ala Tyr Leu Ala Ala Phe Ile Gly  Asn Tyr Leu Leu Glu  Arg Tyr Pro 
        995                 1000                 1005             


Lys Leu  Gln Ser Tyr Phe Val  Tyr Gly Asp Phe Lys  Lys Phe Ser 
    1010                 1015                 1020             


Gln Lys  Asp Thr Lys Met Arg  Arg Phe Asn Phe Ile  Tyr Asp Leu 
    1025                 1030                 1035             


Lys His  Ser Asp Gln Ile Ala  Asp Lys Asn Gly Lys  Lys Ile Trp 
    1040                 1045                 1050             


His Lys  Thr Asn Asp Leu Asp  Tyr Ile Arg His Leu  Phe Ala Tyr 
    1055                 1060                 1065             


Lys Lys  Ile Leu Ile Ser His  Glu Val Ser Glu Arg  Arg Gly Ala 
    1070                 1075                 1080             


Leu Tyr  Asn Gln Thr Val Tyr  Lys Ala Ser Asp Asp  Lys Ala Ser 
    1085                 1090                 1095             


Gly Gln  Gly Ser Lys Lys Leu  Ile Pro Ile Lys Ala  Asn Lys Pro 
    1100                 1105                 1110             


Thr Glu  Ile Tyr Gly Gly Tyr  Thr Gly Lys Thr Leu  Ala Tyr Met 
    1115                 1120                 1125             


Ala Ile  Val Lys Val Ile Lys  Gly Lys Lys Ile Ala  Tyr Lys Val 
    1130                 1135                 1140             


Val Gly  Val Pro Thr Ser Ala  Leu Val Gln Leu Ala  Gly Ile Glu 
    1145                 1150                 1155             


His Gly  Ser Gln Glu Glu Leu  Asn Arg Leu Tyr Asp  Val Leu Lys 
    1160                 1165                 1170             


Pro Gln  Phe Thr Gln Leu Lys  Lys Asn Asn Lys Thr  Gly Glu Ile 
    1175                 1180                 1185             


Ser Glu  Thr Val Ser Lys Phe  Glu Ile Val Ile Pro  Arg Val Lys 
    1190                 1195                 1200             


Tyr Gln  Gln Leu Val Glu Asp  Asp Asp Lys Leu Phe  Met Ile Gly 
    1205                 1210                 1215             


Ser Asp  Thr Tyr Pro Tyr Asn  Ala Gln Gln Leu Val  Leu Ser Glu 
    1220                 1225                 1230             


Gly Ala  Leu Lys Val Ile Asn  Asn Val Tyr Arg Lys  Gly Glu Asn 
    1235                 1240                 1245             


Ser Asp  Lys Gln Glu Ser Tyr  Lys Leu Asp Asp Val  Tyr Asp Glu 
    1250                 1255                 1260             


Ile Ile  Ile Ala Met Lys Lys  Tyr Phe Pro Leu Tyr  Asp Asn Arg 
    1265                 1270                 1275             


Gly Phe  Arg Glu Lys Val Ala  Glu Gly Lys Ser Thr  Phe Glu Asn 
    1280                 1285                 1290             


Leu Pro  Ile Ser Asn Glu Phe  Lys Asn Gly Lys Leu  Thr Lys Ile 
    1295                 1300                 1305             


Gly Lys  Lys Glu Val Ile Asn  Arg Met Leu Gln Gly  Leu His Ala 
    1310                 1315                 1320             


Asn Ala  Thr Met Thr Asp Leu  Lys Ile Leu Gly Ile  Lys Ser Pro 
    1325                 1330                 1335             


Phe Gly  Phe Met Gln Gln Pro  Thr Gly Ile Ala Leu  Ser Glu Asn 
    1340                 1345                 1350             


Ala Gln  Leu Ile Tyr Gln Ser  Pro Thr Asp Leu Phe  Glu Arg Arg 
    1355                 1360                 1365             


Val Gln  Leu Asn Lys Ile Lys  
    1370                 1375 


<210>  1061
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1061

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Tyr Glu Ser Glu Asn Thr Asp Glu Lys Ser Ser Ile Val 
            20                  25                  30          


Arg Leu Gly Val Arg Val Asn Pro Leu Ser Val Asp Glu Lys Gly Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ala Ile Thr Thr Asn Ala Asp Arg Gln Gln Thr 
    50                  55                  60                  


His Gly Ala Arg Ile Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Asn 
65                  70                  75                  80  


Leu Lys Asp Cys Leu Gln Lys Gln Gly Trp Ile Gly Thr Glu Ala Leu 
                85                  90                  95      


Cys Glu Glu Gly Lys Thr Ser Thr Phe Glu Thr Tyr Met Leu Arg Ala 
            100                 105                 110         


Lys Ala Ala Glu Glu Glu Ile Ser Leu His Glu Leu Cys Arg Val Leu 
        115                 120                 125             


Phe Met Met Asn Lys Lys Arg Gly Tyr Lys Ser Asn Arg Lys Ala Asn 
    130                 135                 140                 


Asn Lys Glu Asp Gly Lys Leu Phe Asp Gly Met Thr Val Ala Lys Lys 
145                 150                 155                 160 


Leu Tyr Glu Glu His Leu Thr Pro Ala Glu Tyr Ser Phe Gln Leu Leu 
                165                 170                 175     


Ser Ser Gly Lys Lys Phe Lys Gln Glu Tyr Tyr Arg Ser Asp Leu Lys 
            180                 185                 190         


Glu Glu Phe Asp Arg Ile Trp Asn Glu Gln Gln Lys Tyr Tyr Pro Glu 
        195                 200                 205             


Ile Leu Thr Asp Asp Phe Lys Gln Gln Leu Gln Gly Lys Ser Lys Gly 
    210                 215                 220                 


Asn Thr Ser Lys Ile Phe Leu Gly Lys Tyr Gly Ile Tyr Ser Ala Asp 
225                 230                 235                 240 


Leu Lys Gly Leu Asp Arg Lys Leu Gln Pro Leu Lys Trp Arg Val Glu 
                245                 250                 255     


Ala Leu Lys Gln Gln Val Asp Lys Glu Val Leu Ala Phe Val Ile Ser 
            260                 265                 270         


Asp Leu Asn Gly Gln Ile Ala Ser Thr Ser Gly Leu Leu Gly Ala Ile 
        275                 280                 285             


Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln 
    290                 295                 300                 


Tyr Leu Trp Thr Ser Leu Lys Glu Thr Pro His Val Ser Ile Lys Asn 
305                 310                 315                 320 


Lys Pro Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Lys Ile Trp 
                325                 330                 335     


Glu Thr Gln Ala Thr Tyr His Lys Glu Leu Thr Pro Glu Leu Lys Gln 
            340                 345                 350         


Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
        355                 360                 365             


Lys Gly Leu Ile Ser Val Cys Glu Leu Glu Gln Arg Lys Val Lys Val 
    370                 375                 380                 


Thr Ile Asp Gly Lys Glu Lys Glu Ile Ser Ile Gly Pro Lys Val Ala 
385                 390                 395                 400 


Pro Lys Ser Ser Pro Met Phe Gln Glu Phe Arg Ile Trp Gln Asn Leu 
                405                 410                 415     


Asn Asn Ile Leu Leu Ile Asp Lys Gly Asn Asn Ser Ile His Pro Leu 
            420                 425                 430         


Tyr Glu Glu Glu Lys Asn Leu Leu Tyr Glu Glu Leu Ser Val Lys Glu 
        435                 440                 445             


Lys Leu Ser Lys Ala Glu Ala Leu Lys Val Leu Asn Lys Asn Ser Lys 
    450                 455                 460                 


Gln Trp Asp Leu Asn Tyr Lys Glu Ile Glu Gly Asn Arg Thr Gln Ala 
465                 470                 475                 480 


Val Leu Tyr Asp Cys Tyr Asn Arg Ile Ile Val Met Thr Gly His Glu 
                485                 490                 495     


Glu Tyr Asn Phe Lys Lys Leu Lys Ala Ser Glu Ile Arg Ser Tyr Val 
            500                 505                 510         


Lys Thr Thr Phe Thr Gly Leu Gly Phe Asn Thr Asp Ile Leu Glu Phe 
        515                 520                 525             


Asp Ser Ser Leu Thr Gly His Glu Leu Glu Lys Gln Pro Met Tyr Gln 
    530                 535                 540                 


Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asn Ser Arg Thr Gly 
545                 550                 555                 560 


Asn Asp Ala Leu Leu Arg Lys Leu Glu Thr Ala Phe Gly Phe Pro Lys 
                565                 570                 575     


Glu Tyr Ala Ala Val Leu Ser Asp Val Val Phe Glu Asp Ser Tyr Gly 
            580                 585                 590         


Asn Leu Ser Val Lys Ala Met Arg Lys Ile Leu Pro His Leu Gln Ala 
        595                 600                 605             


Gly Leu Asn Tyr Ser Gln Ala Cys Glu Glu Ala Gly Tyr Lys His Ser 
    610                 615                 620                 


Arg Arg Ser Leu Thr Lys Asn Glu Leu Asp Gln Lys Glu Tyr Lys Glu 
625                 630                 635                 640 


Lys Leu Glu Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
                645                 650                 655     


Lys Ile Leu Asn Gln Met Ile Asn Val Ile Asn Ala Val Ile Asp Glu 
            660                 665                 670         


Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys 
        675                 680                 685             


Ile Gly Ala Ala Glu Arg Glu Asn Met Thr Arg Ser Ile Asn Gln Gly 
    690                 695                 700                 


Asn Ala Glu Asn Lys Arg Ile Arg Glu Ile Leu Glu Lys Glu Phe Ala 
705                 710                 715                 720 


Leu Ser Tyr Ile Ser Arg Asn Asp Ile Ile Lys Tyr Lys Leu Tyr Glu 
                725                 730                 735     


Glu Leu Lys Ser Asn Gly Phe Lys Thr Leu Tyr Ser Asp Thr Tyr Ile 
            740                 745                 750         


Ser Lys Asp Lys Leu Phe Ser Lys Asp Phe Asp Ile Glu His Ile Ile 
        755                 760                 765             


Pro Lys Ala Arg Leu Phe Asp Asp Ser Leu Ser Asn Lys Thr Ile Glu 
    770                 775                 780                 


Ala Arg Asp Val Asn Ile Ala Lys Ser Asn Lys Thr Ala Phe Asp Phe 
785                 790                 795                 800 


Ile Ser Glu Lys Tyr Asp Gln Gln Gly Ile Glu Ala Tyr Lys Lys Lys 
                805                 810                 815     


Leu Asp Ile Leu Leu Gln Asp Gly Ser Ile Ser Lys Ala Lys Tyr Asn 
            820                 825                 830         


Arg Leu Leu Met Lys Glu Ala Asp Ile Pro Ser Gly Phe Ile Asp Arg 
        835                 840                 845             


Asp Leu Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Tyr Glu Ile Leu 
    850                 855                 860                 


Ser Asp Leu Val Arg Asn Val Thr Pro Thr Thr Gly Ala Val Thr Ser 
865                 870                 875                 880 


Arg Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn 
                885                 890                 895     


Phe Glu Lys Tyr Gln Lys Leu Gly Leu Val Glu Val Ile Glu Asp Arg 
            900                 905                 910         


Asp Gly Arg Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp 
        915                 920                 925             


His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr Lys Pro 
    930                 935                 940                 


Ser Tyr Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Ser Asp Lys Asn 
945                 950                 955                 960 


Ser Ser Ile Tyr Gly Ile Glu Asn Lys Glu Leu His Arg Glu Asp Asn 
                965                 970                 975     


Lys Leu Arg Phe Asn Ala Pro Leu Pro Ile Asn Glu Phe Arg Ala Glu 
            980                 985                 990         


Ala Arg Arg His Leu Lys Ser Ile  Leu Val Ser Val Lys  Ala Lys Asn 
        995                 1000                 1005             


Lys Val  Met Thr Gln Asn Val  Asn Val Ile Lys Thr  Lys His Gly 
    1010                 1015                 1020             


Val Ile  Lys Lys Val Gln Leu  Thr Pro Arg Gly Glu  Leu His Asn 
    1025                 1030                 1035             


Ala Thr  Phe Tyr Gly Thr Lys  Lys Arg Pro Ile Ile  Lys Met Val 
    1040                 1045                 1050             


Lys Val  Gly Ala Ala Leu Asp  Glu Val Thr Ile Lys  Lys Val Ser 
    1055                 1060                 1065             


Ser Ser  Phe Ile Arg Glu Ala  Leu Leu Asp Arg Leu  His Glu Tyr 
    1070                 1075                 1080             


Asn Gly  Asp Ala Lys Lys Ala  Phe Thr Gly Arg Asn  Ser Ile Asp 
    1085                 1090                 1095             


Lys Asn  Pro Ile Tyr Ile Asp  Glu Glu His Lys Lys  Thr Val Pro 
    1100                 1105                 1110             


Ala Leu  Val Lys Thr Ile Glu  Trp Glu Met Tyr His  Pro Thr Arg 
    1115                 1120                 1125             


Lys Leu  Ile Ser Pro Asp Leu  Lys Ile Asp Lys Val  Ile Asp Lys 
    1130                 1135                 1140             


Gly Ile  Arg Glu Ile Leu Lys  Thr Arg Leu Ala Lys  Phe Asn Gly 
    1145                 1150                 1155             


Asp Ala  Gln Lys Ala Phe Ser  Asn Leu Asp Glu Asn  Pro Ile Tyr 
    1160                 1165                 1170             


Lys Asp  Glu Ala Lys Lys Ile  Thr Leu Lys Arg Val  Ser Leu Glu 
    1175                 1180                 1185             


Gly Val  Ser Ser Val Ile Pro  Leu His Thr Leu Lys  Asp Arg Glu 
    1190                 1195                 1200             


Gly Lys  Leu Val Val Gly Lys  Asp Gly Lys Pro Val  Leu Gly Asp 
    1205                 1210                 1215             


Tyr Val  Gln Thr Ser Asn Asn  His His Ile Ala Phe  Tyr Met Asp 
    1220                 1225                 1230             


Glu Asn  Gly Asn Leu Gln Asp  Asn Val Val Ser Phe  Phe Glu Ala 
    1235                 1240                 1245             


Ala Glu  Arg Lys Ser Gln Gly  Val Pro Val Ile Asp  Lys Asn Tyr 
    1250                 1255                 1260             


Asn His  Asp Lys Gly Trp Asn  Phe Leu Phe Thr Met  Lys Gln Asn 
    1265                 1270                 1275             


Glu Tyr  Phe Val Phe Pro Asn  Glu Val Thr Gly Phe  Asn Pro Ser 
    1280                 1285                 1290             


Glu Val  Asp Leu Met Asp Glu  Ala Asn Tyr Ser Ile  Ile Ser Pro 
    1295                 1300                 1305             


Asn Leu  Tyr Arg Val Gln Lys  Met Ser Lys Ala Ser  Gly Arg Asp 
    1310                 1315                 1320             


Tyr Trp  Phe Arg His His Leu  Glu Ala Met Leu Asn  Asp Asp Pro 
    1325                 1330                 1335             


Lys Leu  Arg Asn Ser Met Phe  Lys Arg Ile Thr Ser  Leu Lys Ala 
    1340                 1345                 1350             


Leu Glu  Gly Ile Val Lys Val  Arg Ile Asn Cys Ile  Gly Lys Ile 
    1355                 1360                 1365             


Val Ala  Val Gly Glu Tyr Asp  
    1370                 1375 


<210>  1062
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1062

Met Ser Asn Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ser Gln Glu Ile Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Phe Phe Leu Val Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Val Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Ser Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Lys Ser Leu Ser Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Thr Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Lys Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu 
                325                 330                 335     


Lys Gln Phe Ile Gln Asn Asn Leu Gln Glu Lys Tyr Asp Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Lys Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Ile Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Gln Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Gly Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Gln Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Thr Arg Tyr Gln Phe Leu Asp Lys Lys 
    530                 535                 540                 


Gln Lys Lys Asp Ile Phe Asp Thr Phe Phe Lys Ala Glu Asn Lys Arg 
545                 550                 555                 560 


Lys Val Thr Glu Lys Asp Ile Ile His Tyr Leu His Asn Val Asp Gly 
                565                 570                 575     


Tyr Asp Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser 
            580                 585                 590         


Leu Ser Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe 
        595                 600                 605             


Met Asp Asp Ser Lys Asn Glu Glu Ile Leu Glu Asn Ile Ile His Thr 
    610                 615                 620                 


Leu Thr Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln 
625                 630                 635                 640 


Tyr Asp Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Lys Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Glu Ile Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Gly Leu 
    690                 695                 700                 


Ser Phe Lys Glu Ile Ile Gln Lys Ala Gln Val Phe Gly Lys Thr Asn 
705                 710                 715                 720 


Asp Val Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly His Ala Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg 
    770                 775                 780                 


Ile Glu Asp Ser Leu Lys Asn Leu Ala Ser Gly Leu Asp Ser Asn Ile 
785                 790                 795                 800 


Leu Lys Glu Asn Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Lys Pro 
            820                 825                 830         


Leu Glu Ile Asn Gln Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser 
    850                 855                 860                 


Ser Lys Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val 
865                 870                 875                 880 


Val Glu Lys Met Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu 
                885                 890                 895     


Ile Ser Glu Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Glu Arg 
            900                 905                 910         


Gly Gly Leu Asn Glu Leu Asp Lys Val Gly Phe Ile Lys Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Phe Leu Asp Ala 
    930                 935                 940                 


Arg Phe Asn Lys Glu Val Thr Glu Lys Asp Lys Lys Asn Arg Asn Val 
945                 950                 955                 960 


Lys Ile Ile Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu 
                965                 970                 975     


Phe Gly Leu Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Leu  Ala Lys Ala Ile Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Lys  Leu Glu Pro Glu Phe  Val Tyr Gly Asp Tyr  Gln Lys Tyr 
    1010                 1015                 1020             


Asp Leu  Gln Arg Tyr Ile Ser  Lys Ser Arg Glu Pro  Lys Glu Val 
    1025                 1030                 1035             


Glu Lys  Ala Thr Gln Lys Tyr  Phe Phe Tyr Ser Asn  Leu Leu Asn 
    1040                 1045                 1050             


Phe Phe  Lys Glu Glu Val His  Tyr Ala Asp Gly Thr  Ile Val Lys 
    1055                 1060                 1065             


Arg Glu  Asn Ile Glu Tyr Ser  Lys Asp Thr Gly Glu  Ile Ala Trp 
    1070                 1075                 1080             


Asn Lys  Glu Lys Asp Phe Ala  Thr Val Lys Lys Val  Leu Ser Leu 
    1085                 1090                 1095             


Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu Val Gln  Thr Gly Gly 
    1100                 1105                 1110             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly Asn Ser  Asp Lys Leu 
    1115                 1120                 1125             


Ile Pro  Arg Lys Thr Lys Glu  Ile Leu Trp Asp Thr  Thr Lys Tyr 
    1130                 1135                 1140             


Gly Gly  Phe Asp Ser Pro Val  Ile Ala Tyr Ser Ile  Leu Leu Ile 
    1145                 1150                 1155             


Ala Asp  Ile Glu Lys Gly Lys  Ala Lys Lys Leu Lys  Thr Val Lys 
    1160                 1165                 1170             


Thr Leu  Val Gly Ile Thr Ile  Met Glu Lys Ala Thr  Phe Glu Lys 
    1175                 1180                 1185             


Asn Pro  Ile Thr Phe Leu Glu  Asn Lys Gly Tyr His  Asn Val Arg 
    1190                 1195                 1200             


Lys Glu  Asn Ile Leu Cys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu 
    1205                 1210                 1215             


Glu Ser  Gly Arg Arg Arg Met  Leu Ala Ser Ala Lys  Glu Leu Gln 
    1220                 1225                 1230             


Lys Gly  Asn Glu Ile Val Leu  Pro Val Tyr Leu Thr  Thr Leu Leu 
    1235                 1240                 1245             


Tyr His  Ser Lys Asn Val His  Lys Leu Asp Glu Pro  Glu His Leu 
    1250                 1255                 1260             


Glu Tyr  Ile Gln Lys His Arg  Tyr Glu Phe Lys Asp  Leu Leu Asn 
    1265                 1270                 1275             


Leu Val  Ser Glu Phe Ser Gln  Lys Tyr Val Leu Ala  Asp Ala Asn 
    1280                 1285                 1290             


Leu Glu  Lys Ile Lys Asn Leu  Tyr Ala Asp Asn Glu  Gln Ala Asp 
    1295                 1300                 1305             


Ile Glu  Ile Leu Ala Asn Ser  Phe Ile Asn Leu Leu  Thr Phe Thr 
    1310                 1315                 1320             


Ala Leu  Gly Ala Pro Ala Ala  Phe Lys Phe Leu Gly  Lys Asp Val 
    1325                 1330                 1335             


Asp Arg  Lys Arg Tyr Thr Thr  Val Ser Glu Ile Leu  Asn Ala Thr 
    1340                 1345                 1350             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1355                 1360                 1365             


Leu Ser  Lys Leu Gly Glu Asp  
    1370                 1375 


<210>  1063
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1063

Met Glu Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asn Arg Lys Ser Ile Lys Lys Asn Leu Met 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Asn Glu Met Ala Lys Leu Asp Asp Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


Asn Glu Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             


His Arg Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Pro Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Ala 
                165                 170                 175     


Glu Asn Ser Asp Val Ala Lys Leu Phe Tyr Gln Leu Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Ser Pro Leu Asp Glu Ile Glu Val Asp Ala 
        195                 200                 205             


Lys Gly Ile Leu Ser Ala Arg Leu Ser Lys Ser Lys Arg Leu Glu Lys 
    210                 215                 220                 


Leu Ile Ala Val Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Ile Ile Ala Leu Ala Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Thr Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Glu Leu Leu Gly Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Ser Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Ser Asn Ser Glu Val Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Val Lys Arg Tyr Asp Glu His His Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     


Thr Leu Val Arg Gln Gln Phe Pro Glu Lys Tyr Ala Glu Ile Phe Lys 
            340                 345                 350         


Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Val Gly Ile Gly Ile Lys 
        355                 360                 365             


His Arg Lys Arg Thr Thr Lys Leu Ala Thr Gln Glu Glu Phe Tyr Lys 
    370                 375                 380                 


Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Ala Glu Glu Leu Leu 
385                 390                 395                 400 


Ala Lys Leu Asn Arg Asp Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp 
                405                 410                 415     


Asn Gly Ser Ile Pro His Gln Ile His Leu Lys Glu Leu His Ala Ile 
            420                 425                 430         


Leu Arg Arg Gln Glu Glu Phe Tyr Pro Phe Leu Lys Glu Asn Arg Glu 
        435                 440                 445             


Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
    450                 455                 460                 


Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Leu Thr Arg Lys Ser Glu 
465                 470                 475                 480 


Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala 
                485                 490                 495     


Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Glu Gln Leu 
            500                 505                 510         


Pro Asn Lys Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe 
        515                 520                 525             


Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Arg Met 
    530                 535                 540                 


Arg Lys Pro Glu Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp 
545                 550                 555                 560 


Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu 
                565                 570                 575     


Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ile Gly 
            580                 585                 590         


Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu 
        595                 600                 605             


Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp 
    610                 615                 620                 


Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu 
625                 630                 635                 640 


Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys 
                645                 650                 655     


Val Met Lys Gln Leu Lys Arg Arg His Tyr Thr Gly Trp Gly Arg Leu 
            660                 665                 670         


Ser Arg Lys Met Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr 
        675                 680                 685             


Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ser Asn Arg Asn Phe Met 
    690                 695                 700                 


Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys Glu Glu Ile Glu Lys 
705                 710                 715                 720 


Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His Glu Gln Ile Ala Asp 
                725                 730                 735     


Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys 
            740                 745                 750         


Ile Val Asp Glu Leu Val Lys Val Met Gly His Lys Pro Glu Asn Ile 
        755                 760                 765             


Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Thr Lys Gly Leu Gln 
    770                 775                 780                 


Gln Ser Arg Glu Arg Lys Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu 
785                 790                 795                 800 


Glu Ser Gln Ile Leu Lys Glu Asn Pro Val Glu Asn Thr Gln Leu Gln 
                805                 810                 815     


Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr 
            820                 825                 830         


Val Asp Gln Glu Leu Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp 
        835                 840                 845             


His Ile Val Pro Gln Ser Phe Ile Lys Asp Asp Ser Ile Asp Asn Lys 
    850                 855                 860                 


Val Leu Thr Arg Ser Val Glu Asn Arg Gly Lys Ser Asp Asn Val Pro 
865                 870                 875                 880 


Ser Glu Glu Val Val Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu 
                885                 890                 895     


Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala 
            900                 905                 910         


Glu Arg Gly Gly Leu Ser Glu Ala Asp Lys Ala Gly Phe Ile Lys Arg 
        915                 920                 925             


Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu 
    930                 935                 940                 


Asp Ser Arg Met Asn Thr Lys Arg Asp Lys Asn Asp Lys Pro Ile Arg 
945                 950                 955                 960 


Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg 
                965                 970                 975     


Lys Asp Phe Gln Leu Tyr Lys Val Arg Asp Ile Asn Asn Tyr His His 
            980                 985                 990         


Ala His Asp Ala Tyr Leu Asn Ala  Val Val Gly Thr Ala  Leu Ile Lys 
        995                 1000                 1005             


Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val Tyr Gly  Asp Tyr Lys 
    1010                 1015                 1020             


Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys Ser Glu  Gln Glu Ile 
    1025                 1030                 1035             


Gly Lys  Ala Thr Ala Lys Arg  Phe Phe Tyr Ser Asn  Ile Met Asn 
    1040                 1045                 1050             


Phe Phe  Lys Thr Glu Val Lys  Leu Ala Asn Gly Glu  Ile Arg Lys 
    1055                 1060                 1065             


Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr Gly Glu  Val Val Trp 
    1070                 1075                 1080             


Asn Lys  Glu Lys Asp Phe Ala  Thr Val Arg Lys Val  Leu Ala Met 
    1085                 1090                 1095             


Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu Val Gln  Thr Gly Gly 
    1100                 1105                 1110             


Phe Ser  Lys Glu Ser Ile Leu  Ser Lys Arg Glu Ser  Ala Lys Leu 
    1115                 1120                 1125             


Ile Pro  Arg Lys Lys Gly Trp  Asp Thr Arg Lys Tyr  Gly Gly Phe 
    1130                 1135                 1140             


Gly Ser  Pro Thr Val Ala Tyr  Ser Ile Leu Val Val  Ala Lys Val 
    1145                 1150                 1155             


Glu Lys  Gly Lys Ala Lys Lys  Leu Lys Ser Val Lys  Val Leu Val 
    1160                 1165                 1170             


Gly Ile  Thr Ile Met Glu Lys  Gly Ser Tyr Glu Lys  Asp Pro Ile 
    1175                 1180                 1185             


Gly Phe  Leu Glu Ala Lys Gly  Tyr Lys Asp Ile Lys  Lys Glu Leu 
    1190                 1195                 1200             


Ile Phe  Lys Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Glu Asn Gly 
    1205                 1210                 1215             


Arg Arg  Arg Met Leu Ala Ser  Ala Thr Glu Leu Gln  Lys Ala Asn 
    1220                 1225                 1230             


Glu Leu  Val Leu Pro Gln His  Leu Val Arg Leu Leu  Tyr Tyr Thr 
    1235                 1240                 1245             


Gln Asn  Ile Ser Ala Thr Thr  Gly Ser Asn Asn Leu  Gly Tyr Ile 
    1250                 1255                 1260             


Glu Gln  His Arg Glu Glu Phe  Lys Glu Ile Phe Glu  Lys Ile Ile 
    1265                 1270                 1275             


Asp Phe  Ser Glu Lys Tyr Ile  Leu Lys Asn Lys Val  Asn Ser Asn 
    1280                 1285                 1290             


Leu Lys  Ser Ser Phe Asp Glu  Gln Phe Ala Val Ser  Asp Ser Ile 
    1295                 1300                 1305             


Leu Leu  Ser Asn Ser Phe Val  Ser Leu Leu Lys Tyr  Thr Ser Phe 
    1310                 1315                 1320             


Gly Ala  Ser Gly Gly Phe Thr  Phe Leu Asp Leu Asp  Val Lys Gln 
    1325                 1330                 1335             


Gly Arg  Leu Arg Tyr Gln Thr  Val Thr Glu Val Leu  Asp Ala Thr 
    1340                 1345                 1350             


Leu Ile  Tyr Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Thr Asp 
    1355                 1360                 1365             


Leu Ser  Gln Leu Gly Gly Asp  
    1370                 1375 


<210>  1064
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1064

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ser Glu Asn Lys Asp Glu Gln Ser Ser Ile Val 
            20                  25                  30          


Arg Leu Gly Val Arg Val Ile Pro Leu Thr Val Asp Glu Lys Gly Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ala Ile Thr Thr Asn Ala Asp Arg Gln Gln Val 
    50                  55                  60                  


His Ala Ala Arg Ile Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Asn 
65                  70                  75                  80  


Leu Arg Asp Cys Leu Gln Lys Gln Gly Trp Ile Gly Ser Glu Pro Met 
                85                  90                  95      


Cys Glu Asp Gly Lys Ser Ser Thr Phe Glu Thr Tyr Lys Leu Arg Ala 
            100                 105                 110         


Lys Ala Ala Val Glu Glu Val Ser Leu His Glu Phe Ala Arg Val Leu 
        115                 120                 125             


Phe Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Asn Arg Lys Ala Asn 
    130                 135                 140                 


Asp Lys Glu Glu Gly Lys Leu Phe Asp Gly Met Thr Ile Ala Lys Lys 
145                 150                 155                 160 


Leu Tyr Glu Glu His Leu Thr Pro Ala Glu Tyr Ser Leu Gln Leu Leu 
                165                 170                 175     


Asn Thr Gly Arg Lys Phe Thr Pro Glu Tyr Tyr Arg Ser Asp Leu Ile 
            180                 185                 190         


Glu Glu Phe Glu Lys Ile Trp Asn Glu Gln Gln Lys Tyr Tyr Pro Glu 
        195                 200                 205             


Ile Leu Thr Asp Glu Phe Lys Gln Gln Leu Glu Gly Lys Ala Lys Ser 
    210                 215                 220                 


Asn Thr Ser Lys Leu Phe Leu Gly Lys Tyr Gly Ile Tyr Ser Ala Asp 
225                 230                 235                 240 


Thr Lys Gly Leu Asp Arg Arg Leu Gln Pro Leu Lys Trp Arg Val Glu 
                245                 250                 255     


Ala Met Lys Gln Gln Val Asp Lys Asp Val Leu Ala Phe Val Ile Ser 
            260                 265                 270         


Asp Leu Lys Gly Gln Ile Ala Asn Thr Ser Gly Leu Leu Gly Ala Ile 
        275                 280                 285             


Ser Asp Arg Ser Lys Glu Leu Tyr Phe Ser Lys Gln Thr Ile Gly Gln 
    290                 295                 300                 


Tyr Leu Trp Ala Ser Leu Gln Glu Asn Pro His Ile Ser Ile Lys Asn 
305                 310                 315                 320 


Gln Pro Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Lys Ile Trp 
                325                 330                 335     


Glu Thr Gln Ala Thr Tyr His Lys Glu Leu Thr Ser Glu Leu Lys Gln 
            340                 345                 350         


Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
        355                 360                 365             


Lys Asn Leu Ile Ala Val Cys Glu Leu Glu Gln Arg Lys Val Lys Val 
    370                 375                 380                 


Thr Val Asp Gly Lys Glu Lys Glu Ile Thr Ile Gly Pro Lys Ile Ala 
385                 390                 395                 400 


Pro Lys Ser Ser Pro Met Phe Gln Glu Phe Arg Ile Trp Gln Asn Leu 
                405                 410                 415     


Asn Asn Ile Leu Leu Leu Asp His Glu Thr Asn Ser Lys Arg Pro Leu 
            420                 425                 430         


Tyr Glu Glu Glu Arg Asn Leu Leu Tyr Glu Glu Leu Ser Val Lys Glu 
        435                 440                 445             


Lys Leu Ser Lys Thr Glu Ala Leu Lys Val Leu Gly Lys Lys Ser Lys 
    450                 455                 460                 


Gln Trp Asp Leu Asn Tyr Lys Glu Leu Glu Gly Asn Arg Thr Gln Ala 
465                 470                 475                 480 


Val Leu Tyr Asp Cys Tyr Asn Arg Ile Ile Val Met Ser Gly His Glu 
                485                 490                 495     


Glu Cys Asn Phe Lys Lys Leu Lys Ala Ser Asp Ile Arg His Tyr Val 
            500                 505                 510         


Lys Met Ile Phe Lys Asn Leu Ala Phe Asn Thr Asp Ile Leu Cys Phe 
        515                 520                 525             


Asp Ser Ser Leu Lys Gly His Glu Leu Glu Arg Gln Pro Met Cys Gln 
    530                 535                 540                 


Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asn Ser Arg Thr Gly 
545                 550                 555                 560 


Asn Asp Ala Leu Leu Arg Lys Leu Glu Thr Ser Phe Gly Phe Pro Lys 
                565                 570                 575     


Glu Tyr Ala Ala Val Leu Ala Asp Val Ile Phe Glu Asp Ser Tyr Gly 
            580                 585                 590         


Asn Leu Ser Val Lys Ala Met Arg Asn Ile Leu Pro His Leu Gln Ala 
        595                 600                 605             


Gly Lys Asp Tyr Ser Gln Ala Cys Glu Glu Ala Gly Tyr Lys His Ser 
    610                 615                 620                 


Arg Arg Ser Leu Thr Lys Glu Glu Leu Asn Gln Lys Val Tyr Lys Glu 
625                 630                 635                 640 


Lys Leu Glu Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
                645                 650                 655     


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Ala Val Ile Glu Glu 
            660                 665                 670         


Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Asp Leu Lys 
        675                 680                 685             


Ile Gly Ala Ala Glu Arg Glu Asn Met Thr Arg Ser Ile Asn Gln Gly 
    690                 695                 700                 


Asn Ala Glu Asn Lys Arg Ile Arg Glu Ile Leu Glu Lys Glu Phe Ala 
705                 710                 715                 720 


Leu Ser Tyr Leu Ser Arg Asn Asp Ile Ile Lys Tyr Lys Leu Tyr Glu 
                725                 730                 735     


Glu Leu Lys Ser Asn Gly Phe Lys Thr Leu Tyr Ser Asp Thr Tyr Ile 
            740                 745                 750         


Ser Lys Asp Lys Leu Phe Ser Lys Asp Phe Asp Ile Glu His Ile Ile 
        755                 760                 765             


Pro Lys Ala Arg Leu Phe Asp Asp Ser Leu Ser Asn Lys Thr Ile Glu 
    770                 775                 780                 


Ala Arg Asp Val Asn Ile Ala Lys Ser Asn Lys Thr Ala Phe Asp Phe 
785                 790                 795                 800 


Ile Leu Glu Arg Tyr Gly Glu Gln Gly Val Lys Thr Tyr Lys Thr Lys 
                805                 810                 815     


Leu Leu Arg Leu Leu Glu Asp Gly Ala Ile Ser Asn Lys Lys Tyr Asn 
            820                 825                 830         


Asn Leu Leu Lys Thr Glu Ala Asp Ile Pro Ser Gly Phe Ile Asp Arg 
        835                 840                 845             


Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala Tyr Glu Ile Leu 
    850                 855                 860                 


Ser Glu Leu Val Lys Ile Val Thr Pro Thr Thr Gly Ser Val Thr Asn 
865                 870                 875                 880 


Arg Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn 
                885                 890                 895     


Phe Glu Lys Tyr Gln Lys Leu Gly Leu Ala Asp Val Ile Glu Asp Arg 
            900                 905                 910         


Asp Gly Arg Lys Val Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp 
        915                 920                 925             


His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr Lys Pro 
    930                 935                 940                 


Ser Tyr Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Ser Asp Lys Ser 
945                 950                 955                 960 


Ser Ser Ile Tyr Gly Ile Glu Val Lys Glu Leu His Arg Glu Glu Gly 
                965                 970                 975     


Lys Phe Arg Phe Asn Ala Pro Leu Pro Ile Asn Glu Phe Arg Ala Glu 
            980                 985                 990         


Ala Lys Arg His Leu Lys Ser Val  Leu Val Ser Ile Lys  Ala Lys Lys 
        995                 1000                 1005             


Lys Val  Met Thr Gln Asn Val  Asn Lys Ile Lys Thr  Lys His Gly 
    1010                 1015                 1020             


Ile Ile  Lys Lys Val Gln Leu  Thr Pro Arg Gly Glu  Leu His Asn 
    1025                 1030                 1035             


Ala Thr  Tyr Tyr Gly Thr Lys  Lys Arg Pro Leu Val  Arg Met Val 
    1040                 1045                 1050             


Lys Val  Gly Ala Ala Leu Asp  Glu Ala Thr Ile Lys  Arg Val Cys 
    1055                 1060                 1065             


Ser Pro  Ala Ile Arg Ala Ala  Leu Leu Lys Arg Leu  Asn Ala Tyr 
    1070                 1075                 1080             


Asn Gly  Asp Ala Lys Glu Ala  Phe Thr Gly Arg Asn  Ser Ile Asp 
    1085                 1090                 1095             


Lys Asn  Pro Ile Tyr Leu Asp  Ile Glu Gln Thr Lys  Leu Val Pro 
    1100                 1105                 1110             


Val Leu  Val Lys Thr Ile Glu  Trp Gly Met Tyr His  Pro Ile Arg 
    1115                 1120                 1125             


Lys Arg  Ile Thr Pro Asp Leu  Lys Ile Asp Lys Val  Ile Asp Lys 
    1130                 1135                 1140             


Gly Ile  Arg Glu Lys Leu Lys  Ala Arg Leu Ala Glu  Phe Asn Gly 
    1145                 1150                 1155             


Asp Ala  Gln Lys Ala Phe Ser  Asn Leu Asp Glu Asn  Pro Ile Tyr 
    1160                 1165                 1170             


Met Asp  Glu Ala Lys Arg Ile  Thr Leu Lys Arg Val  Ser Ile Glu 
    1175                 1180                 1185             


Gly Val  Leu Ser Ala Ile Pro  Leu His Thr Leu Lys  Asp Lys Ala 
    1190                 1195                 1200             


Gly Lys  Pro Val Ile Gly Ser  Asp Gly Lys Pro Val  Phe Gly Asp 
    1205                 1210                 1215             


Tyr Val  Gln Thr Ser Asn Asn  His His Ile Ala Phe  Tyr Ile Asp 
    1220                 1225                 1230             


Asp Lys  Gly Asn Leu Gln Asp  Asn Pro Val Ser Phe  Phe Glu Ala 
    1235                 1240                 1245             


Ala Glu  Arg Lys Ser Gln Gly  Val Pro Val Ile Asp  Arg Arg Tyr 
    1250                 1255                 1260             


Asn Cys  Asp Lys Gly Trp His  Phe Val Phe Thr Met  Lys Gln Asn 
    1265                 1270                 1275             


Glu Tyr  Phe Val Phe Pro Asn  Glu Ala Thr Gly Phe  Ile Pro Ser 
    1280                 1285                 1290             


Glu Val  Asp Leu Leu Asp Glu  Ala Asn Tyr Cys Ile  Ile Ser Pro 
    1295                 1300                 1305             


Asn Leu  Tyr Arg Val Gln Lys  Leu Ser Lys Ala Ser  Gly Arg Asp 
    1310                 1315                 1320             


Tyr Trp  Phe Arg His His Leu  Glu Thr Met Leu Asn  Asp Asp Leu 
    1325                 1330                 1335             


Arg Leu  Lys Asn Ser Thr Phe  Lys Arg Ile Gln Ser  Leu Lys Gly 
    1340                 1345                 1350             


Leu Glu  Asp Ile Val Lys Val  Arg Ile Asn Ser Ile  Gly Lys Ile 
    1355                 1360                 1365             


Val Ala  Val Gly Lys Tyr Asp  
    1370                 1375 


<210>  1065
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1065

Met Glu Thr Lys Phe Thr Tyr Tyr Leu Gly Leu Asp Met Gly Thr Asp 
1               5                   10                  15      


Ser Leu Gly Trp Ala Val Thr Asp Ser Ser Tyr His Ile Leu Arg Ala 
            20                  25                  30          


Lys Gly Lys Asp Leu Trp Gly Val Arg Leu Phe Pro Ala Ala Glu Thr 
        35                  40                  45              


Ser Val Asp Arg Arg Thr Tyr Arg Val Asn Arg Arg Arg Arg Gln Arg 
    50                  55                  60                  


Glu Val Ala Arg Ile Gly Ala Leu Lys Glu Leu Phe Ala Asp Glu Ile 
65                  70                  75                  80  


Glu Lys Val Asp Pro Gly Phe Phe Val Arg Leu Asp Glu Ser Lys Phe 
                85                  90                  95      


His Met Glu Asp Arg Ser Glu Asn Asn Lys Gln Lys Tyr Gly Leu Phe 
            100                 105                 110         


Ala Asp Lys Leu Phe Ser Asp Ala Glu Tyr Tyr Lys Lys Tyr Pro Thr 
        115                 120                 125             


Ile Phe His Leu Arg Lys Glu Leu Leu Glu Ser Glu Glu Pro His Asp 
    130                 135                 140                 


Val Arg Leu Val Tyr Leu Ala Leu Leu Asn Met Phe Lys His Arg Gly 
145                 150                 155                 160 


His Phe Leu Asn Ser Ser Leu Gln Ile Glu Gly Glu Asn Ser Asn Ile 
                165                 170                 175     


Ala Glu Ser Tyr Gln Met Phe Val Asp Thr Leu Lys Asp Glu Glu Leu 
            180                 185                 190         


Asp Phe Pro Glu Gln Val Ser Gly Glu Arg Leu Gln Glu Ile Phe Arg 
        195                 200                 205             


Ser Ser Asp Ser Arg Lys Lys Lys Val Glu Asn Leu Leu Ala Glu Met 
    210                 215                 220                 


Asn Leu Ser Lys Lys Gln Lys Arg Glu Ile Gln Phe Val Asn Leu Ile 
225                 230                 235                 240 


Cys Gly Leu Lys Gly Lys Leu Ser Glu Leu Phe Pro Asp Lys Glu Tyr 
                245                 250                 255     


Asp Glu Glu Gln Lys Lys Ile Ala Phe Ser Phe Gln Asp Ser Asp Tyr 
            260                 265                 270         


Glu Asp Lys Glu Asn Thr Ile Gln Glu Leu Leu Asp Glu Ser Glu Met 
        275                 280                 285             


Asn Met Ile Tyr Ala Val Lys Ala Val His Asp Ser Gly Val Leu Ser 
    290                 295                 300                 


Gly Ile Met Asn Gly Glu Arg Tyr Leu Ser Val Ala Arg Val Ala Ser 
305                 310                 315                 320 


Tyr Glu Lys His Lys Lys Asp Leu Gln Ile Leu Lys Asp Ile Leu Lys 
                325                 330                 335     


Lys Tyr Asp Thr Lys Glu Tyr Phe Glu Met Phe Arg Asn Glu Ser Glu 
            340                 345                 350         


Lys Ser Glu Asn Tyr Ser Ala Tyr Val Gly Ser Val Lys Tyr His Gly 
        355                 360                 365             


Thr Phe Arg Arg Gly Leu Ser Gly Gly Lys Gln Glu Asp Leu Tyr Lys 
    370                 375                 380                 


His Ile Lys Lys Val Leu Gly Lys Phe Pro Gln Asp Asp Glu Gly Val 
385                 390                 395                 400 


Thr Tyr Val Leu Glu Glu Ile Glu Thr Glu Ser Phe Leu Pro Lys Gln 
                405                 410                 415     


Leu Thr Ala Ser Asn Gly Val Ile Pro Asn Gln Val His Ala Arg Glu 
            420                 425                 430         


Met Leu Ala Ile Leu Lys His Ala Glu Leu Tyr Leu Pro Phe Leu Gln 
        435                 440                 445             


Glu Lys Asp Asp Thr Gly Leu Ser Val Ser Glu Lys Ile Leu Lys Leu 
    450                 455                 460                 


Phe Met Phe Gln Ile Pro Tyr Tyr Val Gly Pro Leu Gly Gln Glu Tyr 
465                 470                 475                 480 


Lys Asp Lys Lys Gly Tyr Asn Val Trp Ala Glu Arg Lys Glu Pro Gly 
                485                 490                 495     


Lys Ile Tyr Pro Trp Asn Phe Glu Glu Lys Ile Asp Val Lys Lys Ser 
            500                 505                 510         


Ala Glu Lys Phe Ile Gly Arg Met Val Arg Arg Cys Thr Tyr Leu Ser 
        515                 520                 525             


Gly Glu Gln Ala Leu Pro Lys Gln Ser Leu Leu Tyr Glu Lys Phe Gln 
    530                 535                 540                 


Val Leu Asn Glu Leu Asn Asn Leu Arg Ile Arg Gly Glu Lys Ile Pro 
545                 550                 555                 560 


Val Ser Leu Lys Gln Glu Ile Tyr His Asp Leu Phe Gly Asp Gly Lys 
                565                 570                 575     


Lys Val Ser Met Thr Arg Leu Thr Asn Tyr Leu Leu Gln Asn Gly Val 
            580                 585                 590         


Ile Ser Ser Glu Glu Lys Asp Ser Cys Ile Ser Gly Ile Asp Gly Gly 
        595                 600                 605             


Phe His Asn Ser Leu Ser Ser Val Gly Lys Phe Lys Gly Val Phe Gly 
    610                 615                 620                 


Asp Thr Val His Thr Asp Glu His Arg Lys Met Ile Glu Asp Ile Ile 
625                 630                 635                 640 


Phe Trp Gly Thr Val Phe Gly Asp Gly Lys Lys Leu Leu Lys Glu Lys 
                645                 650                 655     


Val Glu Glu Val Tyr Gly Asn Gln Leu Thr Glu Ala Gln Lys Lys Arg 
            660                 665                 670         


Ile Phe Gly Phe Lys Phe Ser Gly Trp Gly Arg Leu Ser Lys Ala Phe 
        675                 680                 685             


Leu Thr Met Glu Gly Ala Ser Lys Asp Asn Gly Glu Ile Lys Gly Leu 
    690                 695                 700                 


Ile Gln Ala Met Trp Asp Thr Asn Cys Asn Leu Met Glu Leu Leu Ser 
705                 710                 715                 720 


Glu Arg Phe Thr Tyr Lys Asp Lys Leu Asn Glu Met Val Thr Thr Ala 
                725                 730                 735     


Glu Lys Pro Leu Cys Glu Trp Lys Ile Glu Asp Leu Asp Glu Met Tyr 
            740                 745                 750         


Leu Ser Ala Pro Val Lys Arg Met Val Trp Gln Thr Ile Arg Val Val 
        755                 760                 765             


Asp Glu Val Gln Gln Ile Met Gly Cys Pro Pro Lys Arg Ile Phe Val 
    770                 775                 780                 


Glu Met Thr Arg Ser Asp Gly Glu Lys Gly Lys Arg Thr Val Ser Arg 
785                 790                 795                 800 


Gln Lys Lys Leu Val Asp Leu Tyr Lys Ala Leu Gly Lys Glu Gly Lys 
                805                 810                 815     


Ile Trp Ser Glu Glu Leu Asn Asn Thr Pro Glu Gly Gln Phe Arg Ile 
            820                 825                 830         


Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln Met Gly Lys Cys Met Tyr Thr 
        835                 840                 845             


Gly Glu Ser Ile Glu Leu Glu Pro Leu Met Asn Asp Asn His Leu Tyr 
    850                 855                 860                 


Asp Ile Asp His Ile Tyr Pro Arg His Phe Ile Lys Asp Asp Asn Ile 
865                 870                 875                 880 


Ser Asn Asn Leu Val Leu Val Lys Lys Glu Arg Asn Ala His Lys Ser 
                885                 890                 895     


Asp Ser Phe Pro Ile Glu Pro Glu Ile Arg Asn Lys Met Met Pro Phe 
            900                 905                 910         


Trp Lys Ser Leu Arg Lys Lys Gly Phe Leu Thr Glu Glu Lys Tyr Asn 
        915                 920                 925             


Arg Leu Thr Arg Met Ser Ala Phe Thr Asp Glu Glu Lys Ala Ala Phe 
    930                 935                 940                 


Ile Ser Arg Gln Leu Val Glu Thr Ser Gln Gly Thr Lys Ala Ile Thr 
945                 950                 955                 960 


Gln Ile Phe Lys Gln Ala Phe Ala Asp Ser Glu Ile Val Phe Ser Lys 
                965                 970                 975     


Ala Ser Val Val Ser Ala Phe Arg Gln Lys Phe Asp Cys Tyr Lys Val 
            980                 985                 990         


Arg Cys Val Asn Asp Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ile 
        995                 1000                 1005             


Val Ala  Gly Asn Thr Tyr Arg  Val Lys Phe Thr Ser  Asn Pro Leu 
    1010                 1015                 1020             


Asn Phe  Ile Lys Glu Ala Lys  Lys Asn Pro Asn Ser  Arg Glu Asn 
    1025                 1030                 1035             


Arg Tyr  Asn Met Asp Lys Thr  Phe Glu Trp Asp Val  Thr Arg Asn 
    1040                 1045                 1050             


Gly Glu  Thr Ala Trp Val Ala  Gln Ser Asn Glu Asn  Pro Gly Thr 
    1055                 1060                 1065             


Ile Gln  Leu Val Lys Lys Tyr  Leu Asn Lys Asn Ser  Pro Leu Ile 
    1070                 1075                 1080             


Thr Lys  Met Val Tyr Glu Lys  His Gly Gly Ile Thr  Gln Lys Ala 
    1085                 1090                 1095             


Thr Ile  Trp Ser Ala Lys Lys  Ala Glu Ser Gly Asn  Thr Asp Ala 
    1100                 1105                 1110             


Tyr Ile  Pro Val Lys Met Asn  Asp Pro Arg Leu Ala  Asp Val Thr 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Val Thr Thr  Ile Ala Val Ala Gly  Tyr Thr Leu 
    1130                 1135                 1140             


Leu Glu  Tyr Lys Ala Gly Gly  Lys Thr Val Arg Ser  Leu Glu Ala 
    1145                 1150                 1155             


Leu Pro  Ile Tyr Leu Gly Gly  Ser Lys Asn Leu Thr  Glu Glu Gln 
    1160                 1165                 1170             


Met Val  Glu Tyr Phe Ser Asn  Ile Leu Gln Gln Glu  Asn Lys Glu 
    1175                 1180                 1185             


Lys Val  Val Ser Asp Val Ile  Ile Arg Lys Lys Met  Ile Pro Ser 
    1190                 1195                 1200             


Gly Ser  Leu Ile Lys Tyr Asn  Gly Phe Tyr Tyr Tyr  Leu Ala Gly 
    1205                 1210                 1215             


Lys Thr  Gly Asn Arg Ile Val  Leu Ile Ser Ala Val  Gln Leu Cys 
    1220                 1225                 1230             


Leu Pro  Met Asp Thr Met Leu  Tyr Ile Lys Lys Ile  Glu Lys Ala 
    1235                 1240                 1245             


Cys Ser  Thr Gly Asn Phe Glu  Glu Lys Asp Lys Asn  Gly Lys Gln 
    1250                 1255                 1260             


Val Leu  Ser Lys Gln Gln Asn  Glu Phe Val Tyr Glu  Leu Ile Ile 
    1265                 1270                 1275             


Glu Lys  Tyr Gln Gln Ser Ile  Phe Lys Lys Lys Val  Gly Ala Ile 
    1280                 1285                 1290             


Gly Asp  Thr Ile Ile Glu Lys  Arg Glu Lys Phe Ala  Glu Ile Ser 
    1295                 1300                 1305             


Leu Glu  Glu Gln Cys Lys Val  Leu Met Gln Ile Phe  Leu Asn Phe 
    1310                 1315                 1320             


Gln Ser  Gly Ile Gly Val Asp  Leu Thr Ser Val Asp  Gly Ser Asn 
    1325                 1330                 1335             


Lys Ser  Gly Val Thr Tyr Leu  Asn Lys Lys Ile Met  Asn Ala Gln 
    1340                 1345                 1350             


Glu Leu  Lys Leu Ile Asp Gln  Ser Val Thr Gly Val  Phe Ser Arg 
    1355                 1360                 1365             


Glu Ile  Asp Leu Leu Thr Ile  
    1370                 1375 


<210>  1066
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1066

Met Ser Asn Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Ser Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Glu Ser Ser Leu Ile Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Gln Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Leu Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Arg Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Lys Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu His Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu 
                325                 330                 335     


Lys Gln Phe Ile Gln Asn Asn Leu Gln Glu Lys Tyr Asp Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asn Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Leu His Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Ile Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Glu Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Thr Arg Tyr Gln Phe Leu Asp Lys Lys 
    530                 535                 540                 


Gln Lys Lys Asp Ile Phe Tyr Thr Phe Phe Lys Ala Glu Asp Lys Arg 
545                 550                 555                 560 


Lys Val Thr Glu Lys Asp Ile Ile His Tyr Leu His Thr Val Asp Gly 
                565                 570                 575     


Tyr Asp Gly Ile Glu Leu Glu Gly Ile Glu Lys Gln Phe Asn Ala Ser 
            580                 585                 590         


Leu Ser Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe 
        595                 600                 605             


Met Asp Asp Asp Lys Asn Glu Ala Ile Leu Glu Asn Ile Val His Thr 
    610                 615                 620                 


Leu Thr Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln 
625                 630                 635                 640 


Tyr Asn Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Lys Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Glu Ile Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Gly Leu 
    690                 695                 700                 


Ser Phe Lys Glu Ile Ile Gln Lys Ala Gln Val Phe Gly Lys Thr Asn 
705                 710                 715                 720 


Asp Val Lys Gln Val Val His Glu Leu Pro Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly His Ala Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg 
    770                 775                 780                 


Ile Glu Asp Ser Leu Lys Asn Leu Ala Ser Gly Leu Asp Ser Asn Ile 
785                 790                 795                 800 


Leu Lys Glu Asn Pro Thr Asp Asn Val Gln Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Lys Pro 
            820                 825                 830         


Leu Glu Ile Asn Gln Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser 
    850                 855                 860                 


Ser Lys Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val 
865                 870                 875                 880 


Val Glu Lys Met Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu 
                885                 890                 895     


Ile Ser Glu Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Glu Arg 
            900                 905                 910         


Gly Gly Leu Asn Glu Leu Asp Lys Val Gly Phe Ile Lys Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Thr 
    930                 935                 940                 


Arg Phe Asn Lys Glu Val Thr Glu Lys Asp Lys Lys Asn Arg Asn Val 
945                 950                 955                 960 


Lys Ile Ile Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu 
                965                 970                 975     


Phe Gly Leu Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Lys Ala Ile Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Lys  Leu Glu Pro Glu Phe  Val Tyr Gly Glu Tyr  Gln Lys Tyr 
    1010                 1015                 1020             


Asp Leu  Lys Arg Tyr Ile Ser  Arg Ser Lys Asp Pro  Lys Asp Ile 
    1025                 1030                 1035             


Glu Lys  Ala Thr Glu Lys Tyr  Phe Phe Tyr Ser Asn  Leu Leu Asn 
    1040                 1045                 1050             


Phe Phe  Lys Glu Glu Val Gln  Tyr Ala Asp Gly Thr  Ile Ala Lys 
    1055                 1060                 1065             


Arg Glu  Asn Ile Glu Tyr Ser  Lys Asp Thr Gly Glu  Ile Ala Trp 
    1070                 1075                 1080             


Asn Lys  Glu Lys Asp Phe Ala  Thr Val Lys Lys Val  Leu Ser Leu 
    1085                 1090                 1095             


Pro Gln  Val Asn Ile Val Lys  Lys Arg Glu Val Gln  Thr Gly Gly 
    1100                 1105                 1110             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly Asn Ser  Asp Lys Leu 
    1115                 1120                 1125             


Ile Pro  Arg Lys Thr Lys Asp  Ile Leu Trp Glu Thr  Thr Lys Tyr 
    1130                 1135                 1140             


Gly Gly  Phe Asp Ser Pro Val  Ile Ala Tyr Ser Ile  Leu Leu Ile 
    1145                 1150                 1155             


Ala Asp  Ile Glu Lys Gly Lys  Ser Lys Glu Leu Lys  Thr Val Lys 
    1160                 1165                 1170             


Thr Leu  Val Gly Ile Thr Ile  Met Glu Lys Ala Thr  Phe Glu Glu 
    1175                 1180                 1185             


Asn Pro  Ile Thr Phe Leu Glu  Asn Lys Gly Tyr His  Asn Val Arg 
    1190                 1195                 1200             


Lys Glu  Asn Ile Leu Cys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu 
    1205                 1210                 1215             


Glu Asn  Gly Arg Arg Arg Leu  Leu Ala Ser Ala Lys  Glu Leu Gln 
    1220                 1225                 1230             


Lys Gly  Asn Glu Ile Val Leu  Pro Val Tyr Leu Thr  Thr Leu Leu 
    1235                 1240                 1245             


Tyr His  Ser Lys Asn Leu His  Lys Leu Asp Glu Pro  Gly His Leu 
    1250                 1255                 1260             


Glu Tyr  Ile Gln Lys His Arg  Asn Glu Phe Lys Asp  Leu Leu Asn 
    1265                 1270                 1275             


Leu Val  Ser Glu Phe Ser Gln  Lys Tyr Ile Leu Ala  Asp Ala Asn 
    1280                 1285                 1290             


Leu Glu  Lys Ile Gln Asn Leu  Tyr Ala Asp Asn Glu  Gln Ala Asp 
    1295                 1300                 1305             


Ile Glu  Ile Leu Ala Asn Ser  Phe Ile Asn Leu Leu  Thr Phe Thr 
    1310                 1315                 1320             


Thr Leu  Gly Ala Pro Ala Ala  Phe Lys Phe Phe Gly  Lys Asp Ile 
    1325                 1330                 1335             


Asp Arg  Lys Arg Tyr Thr Thr  Val Ser Glu Ile Leu  Asn Ala Thr 
    1340                 1345                 1350             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1355                 1360                 1365             


Leu Ser  Lys Leu Gly Glu Asp  
    1370                 1375 


<210>  1067
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1067

Met Glu Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Val Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Lys Glu Met Thr Lys Val Asp Glu Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         


Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             


His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 


Ser Gln Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     


Glu Asn Thr Asp Val Gln Lys Leu Phe Asn Val Phe Val Glu Thr Tyr 
            180                 185                 190         


Asp Lys Ile Val Asp Glu Ser His Leu Ser Glu Ile Glu Val Asp Ala 
        195                 200                 205             


Ser Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Lys Gln Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     


Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Asp Leu Glu Glu Leu Leu Gly Lys Val Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             


Leu Phe Ile Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     


Glu Phe Ile Lys Ile Asn Lys Leu Lys Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         


Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Asn Gly Val Lys 
        355                 360                 365             


Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Thr Ile Leu Thr Lys Ile Asp 
    370                 375                 380                 


Asp Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Asp Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met His Ser Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         


Leu Lys Glu Asn Gln Ala Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 


Ala Asn Tyr His Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 


Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     


Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


His Val Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             


Tyr Val Asn Glu Gln Gly Glu Ser Phe Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 


Ala Lys Leu Leu Ser Tyr Leu Asn Asn Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     


Asn Asp Leu Ile Gly Leu Asp Lys Asp Ser Lys Ser Phe Asn Ala Ser 
            580                 585                 590         


Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys Ser Phe Leu 
        595                 600                 605             


Asp Asp Lys Thr Asn Gly Gln Ile Ile Glu Asp Ile Val Leu Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr 
625                 630                 635                 640 


Ser Asp Phe Phe Thr Ser Gln Gln Leu Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Asn Asn Lys Thr Ile Leu Asp Phe Leu Ile Asp Asp Gly 
        675                 680                 685             


His Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Glu Ser Leu Ser 
    690                 695                 700                 


Phe Lys Thr Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp 
705                 710                 715                 720 


Ile Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gly Tyr Gly Arg Asn Lys Ser Asn Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 


Gln Asp Ser Leu Lys Glu Phe Gly Ser Asp Ile Leu Ser Lys Lys Lys 
785                 790                 795                 800 


Pro Ser Tyr Val Asp Ser Lys Val Glu Asn Ser His Leu Gln Asn Asp 
                805                 810                 815     


Arg Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr Thr Gly 
            820                 825                 830         


Glu Glu Leu Asp Ile Asp Arg Leu Ser Asp Tyr Asp Ile Asp His Ile 
        835                 840                 845             


Ile Pro Gln Ala Phe Ile Lys Asp Asn Ser Ile Asp Asn Lys Val Leu 
    850                 855                 860                 


Thr Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Ile 
865                 870                 875                 880 


Glu Ile Val Arg Asn Arg Arg Ser Tyr Trp Tyr Lys Leu Tyr Lys Ser 
                885                 890                 895     


Gly Leu Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
            900                 905                 910         


Gly Gly Leu Thr Glu Ala Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala 
    930                 935                 940                 


Arg Phe Asn Thr Lys Arg Asp Glu Asn Asp Lys Val Ile Arg Asp Val 
945                 950                 955                 960 


Lys Val Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Glu 
                965                 970                 975     


Phe Lys Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Gly Thr Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Lys  Leu Thr Pro Glu Phe  Val Tyr Gly Glu Tyr  Lys Lys Tyr 
    1010                 1015                 1020             


Asp Val  Arg Lys Leu Ile Ala  Lys Ser Ser Asp Asp  Tyr Ser Glu 
    1025                 1030                 1035             


Met Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe Tyr Ser  Asn Leu Met 
    1040                 1045                 1050             


Asn Phe  Phe Lys Thr Glu Val  Lys Tyr Ala Asp Gly  Arg Val Phe 
    1055                 1060                 1065             


Glu Arg  Pro Asp Ile Glu Thr  Asn Ala Asp Gly Glu  Val Val Trp 
    1070                 1075                 1080             


Asn Lys  Gln Lys Asp Phe Asp  Ile Val Arg Lys Val  Leu Ser Tyr 
    1085                 1090                 1095             


Pro Gln  Val Asn Ile Val Lys  Lys Val Glu Ala Gln  Thr Gly Gly 
    1100                 1105                 1110             


Phe Ser  Lys Glu Ser Ile Leu  Ser Lys Gly Asp Ser  Asp Lys Leu 
    1115                 1120                 1125             


Ile Pro  Arg Lys Thr Lys Lys  Val Tyr Trp Asn Thr  Lys Lys Tyr 
    1130                 1135                 1140             


Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr Ser Val  Leu Val Val 
    1145                 1150                 1155             


Ala Asp  Ile Glu Lys Gly Lys  Ala Lys Lys Leu Lys  Thr Val Lys 
    1160                 1165                 1170             


Glu Leu  Val Gly Ile Ser Ile  Met Glu Arg Ser Phe  Phe Glu Glu 
    1175                 1180                 1185             


Asn Pro  Val Ser Phe Leu Glu  Lys Lys Gly Tyr His  Asn Val Gln 
    1190                 1195                 1200             


Glu Asp  Lys Leu Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Phe 
    1205                 1210                 1215             


Glu Gly  Gly Arg Arg Arg Leu  Leu Ala Ser Ala Thr  Glu Leu Gln 
    1220                 1225                 1230             


Lys Gly  Asn Glu Val Met Leu  Pro Ala His Leu Val  Glu Leu Leu 
    1235                 1240                 1245             


Tyr His  Ala His Arg Ile Asp  Ser Phe Asn Ser Thr  Glu His Leu 
    1250                 1255                 1260             


Lys Tyr  Val Ser Glu His Lys  Lys Glu Phe Glu Lys  Val Leu Ser 
    1265                 1270                 1275             


Cys Val  Glu Asn Phe Ser Asn  Leu Tyr Val Asp Val  Glu Lys Asn 
    1280                 1285                 1290             


Leu Ser  Lys Val Arg Ala Ala  Ala Glu Ser Met Thr  Asn Phe Ser 
    1295                 1300                 1305             


Leu Glu  Glu Ile Ser Ala Ser  Phe Ile Asn Leu Leu  Thr Leu Thr 
    1310                 1315                 1320             


Ala Leu  Gly Ala Pro Ala Asp  Phe Asn Phe Leu Gly  Glu Lys Ile 
    1325                 1330                 1335             


Pro Arg  Lys Arg Tyr Thr Ser  Thr Lys Glu Cys Leu  Ser Ala Thr 
    1340                 1345                 1350             


Leu Ile  His Gln Ser Val Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1355                 1360                 1365             


Leu Ser  Lys Leu Gly Glu Glu  
    1370                 1375 


<210>  1068
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1068

Met Thr Lys Ile Lys Asp Asp Tyr Ile Val Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asp Ser Cys Gly Trp Val Ala Met Asn Ser Asn Asn Asp Ile Leu Lys 
            20                  25                  30          


Leu Gln Gly Lys Thr Ala Ile Gly Ser Arg Leu Phe Glu Gly Gly Lys 
        35                  40                  45              


Ser Ala Ala Glu Arg Arg Leu Phe Arg Thr Thr His Arg Arg Ile Lys 
    50                  55                  60                  


Arg Arg Arg Trp Arg Leu Lys Leu Leu Glu Glu Phe Phe Asp Pro Tyr 
65                  70                  75                  80  


Met Ala Glu Val Asp Pro Tyr Phe Phe Ala Arg Leu Lys Glu Ser Gly 
                85                  90                  95      


Leu Ser Pro Leu Asp Lys Arg Lys Thr Val Ser Ser Ile Val Phe Pro 
            100                 105                 110         


Thr Ser Ala Glu Asp Lys Lys Phe Tyr Asp Asp Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Tyr Lys Leu Met Thr Glu Asp Glu Lys Phe Asp Leu Arg 
    130                 135                 140                 


Glu Val Tyr Leu Ala Ile His His Ile Ile Lys Tyr Arg Gly Asn Phe 
145                 150                 155                 160 


Leu Tyr Asn Thr Ser Val Lys Asp Phe Lys Ala Ser Lys Ile Asp Val 
                165                 170                 175     


Lys Ser Ser Ile Glu Lys Leu Asn Glu Leu Tyr Glu Asn Leu Gly Leu 
            180                 185                 190         


Asp Leu Asn Val Glu Phe Asn Ile Ser Asn Thr Ala Glu Ile Glu Lys 
        195                 200                 205             


Val Leu Lys Asp Lys Gln Ile Phe Lys Arg Asp Lys Val Lys Lys Ile 
    210                 215                 220                 


Ala Glu Leu Phe Ala Ile Lys Thr Asp Asn Lys Glu Gln Ser Lys Arg 
225                 230                 235                 240 


Ile Lys Asp Ile Ser Lys Gln Val Ala Asn Ala Val Leu Gly Tyr Lys 
                245                 250                 255     


Thr Arg Phe Asp Thr Ile Ala Leu Lys Glu Ile Ser Lys Asp Glu Leu 
            260                 265                 270         


Ser Asp Trp Asn Phe Lys Leu Ser Asp Ile Asp Ala Asp Ser Lys Phe 
        275                 280                 285             


Glu Ala Leu Met Gly Asn Leu Asp Glu Asn Glu Gln Ala Ile Leu Leu 
    290                 295                 300                 


Thr Ile Lys Glu Leu Phe Asn Glu Val Thr Leu Asn Gly Ile Val Glu 
305                 310                 315                 320 


Asp Gly Asn Thr Leu Ser Glu Ser Met Ile Asn Lys Tyr Asn Asp His 
                325                 330                 335     


Arg Asp Asp Leu Lys Leu Leu Lys Glu Val Ile Glu Asn His Ile Asp 
            340                 345                 350         


Arg Lys Lys Ala Lys Glu Leu Ala Leu Ala Tyr Asp Leu Tyr Val Asn 
        355                 360                 365             


Asn Arg His Gly Gln Leu Leu Gln Ala Lys Lys Lys Leu Gly Lys Ile 
    370                 375                 380                 


Lys Pro Arg Ser Lys Glu Asp Phe Tyr Lys Val Val Asn Lys Asn Leu 
385                 390                 395                 400 


Asp Asp Ser Arg Ala Ser Lys Glu Ile Lys Lys Lys Ile Glu Leu Asp 
                405                 410                 415     


Ser Phe Met Pro Lys Gln Arg Thr Asn Ala Asn Gly Val Ile Pro Tyr 
            420                 425                 430         


Gln Leu Gln Gln Leu Glu Leu Asp Lys Ile Ile Glu Asn Gln Ser Lys 
        435                 440                 445             


Tyr Tyr Pro Phe Leu Lys Glu Ile Asn Pro Val Ser Ser His Leu Lys 
    450                 455                 460                 


Glu Ala Pro Tyr Lys Leu Asp Glu Leu Ile Arg Phe Arg Val Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ile Ser Pro Asn Glu Ser Thr Lys Asp Ile Gln 
                485                 490                 495     


Thr Lys Lys Asn Gln Asn Phe Ala Trp Met Ile Arg Lys Glu Glu Gly 
            500                 505                 510         


Arg Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Ile Glu Ser 
        515                 520                 525             


Ala Asn Lys Phe Ile Lys Arg Met Thr Thr Lys Asp Thr Tyr Leu Phe 
    530                 535                 540                 


Gly Glu Asp Val Leu Pro Ala Asn Ser Leu Leu Tyr Gln Lys Phe Thr 
545                 550                 555                 560 


Val Leu Asn Glu Leu Asn Asn Ile Arg Ile Asn Gly Lys Arg Ile Ser 
                565                 570                 575     


Val Asp Leu Lys Gln Glu Ile Tyr Glu Asn Leu Phe Lys Lys His Thr 
            580                 585                 590         


Thr Val Thr Val Lys Lys Leu Glu Asn Tyr Leu Lys Glu Asn His Asn 
        595                 600                 605             


Leu Val Lys Val Glu Ile Lys Gly Leu Ala Asp Glu Lys Lys Phe Asn 
    610                 615                 620                 


Ser Gly Leu Thr Thr Tyr Asn Arg Phe Lys Asn Leu Asn Ile Phe Asp 
625                 630                 635                 640 


Asn Gln Ile Asp Asp Leu Lys Tyr Arg Asn Asp Phe Glu Lys Ile Ile 
                645                 650                 655     


Glu Trp Ser Thr Ile Phe Glu Asp Lys Ser Ile Tyr Lys Glu Lys Leu 
            660                 665                 670         


Arg Ser Ile Asp Trp Leu Asn Glu Lys Gln Ile Asn Ala Leu Ser Asn 
        675                 680                 685             


Ile Arg Leu Gln Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Ala Gln 
    690                 695                 700                 


Leu His Asp His Asn Gly Gln Thr Ile Ile Glu Gln Leu Trp Asp Ser 
705                 710                 715                 720 


Gln Asn Asn Phe Met Gln Ile Val Thr Gln Ala Asp Phe Lys Asp Ala 
                725                 730                 735     


Ile Ala Lys Ala Asn Gln Asn Leu Leu Val Ala Thr Ser Val Glu Asp 
            740                 745                 750         


Ile Leu Asn Asn Ala Tyr Thr Ser Pro Ala Asn Lys Lys Ala Ile Arg 
        755                 760                 765             


Gln Val Ile Lys Val Val Asp Asp Ile Val Lys Ala Ala Ser Gly Lys 
    770                 775                 780                 


Val Pro Lys Gln Ile Ala Ile Glu Phe Thr Arg Asp Ala Asp Glu Asn 
785                 790                 795                 800 


Pro Lys Arg Ser Gln Thr Arg Gly Ser Lys Leu Gln Lys Val Tyr Lys 
                805                 810                 815     


Asp Leu Ser Thr Glu Leu Ala Ser Lys Thr Ile Ala Glu Glu Leu Asn 
            820                 825                 830         


Glu Ala Ile Lys Asp Lys Lys Leu Val Gln Asp Lys Tyr Tyr Leu Tyr 
        835                 840                 845             


Phe Met Gln Leu Gly Arg Asp Ala Tyr Thr Gly Glu Pro Ile Asn Ile 
    850                 855                 860                 


Asp Glu Ile Gln Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Phe 
865                 870                 875                 880 


Ile Lys Asp Asp Ala Leu Asp Asn Arg Val Leu Val Ser Arg Ala Val 
                885                 890                 895     


Asn Asn Gly Lys Ser Asp Asn Val Pro Val Lys Leu Phe Gly Asn Glu 
            900                 905                 910         


Met Ala Ala Asn Leu Gly Met Thr Ile Arg Lys Met Trp Glu Glu Trp 
        915                 920                 925             


Lys Asn Ile Gly Leu Ile Ser Lys Thr Lys Tyr Asn Asn Leu Leu Thr 
    930                 935                 940                 


Asp Pro Asp His Ile Asn Lys Tyr Lys Ser Ala Gly Phe Ile Arg Arg 
945                 950                 955                 960 


Gln Leu Val Glu Thr Ser Gln Ile Ile Lys Leu Val Ser Thr Ile Leu 
                965                 970                 975     


Gln Ser Arg Tyr Pro Asn Thr Glu Ile Ile Thr Val Lys Ala Lys Tyr 
            980                 985                 990         


Asn His Tyr Leu Arg Glu Lys Phe  Asp Leu Tyr Lys Ser  Arg Glu Val 
        995                 1000                 1005             


Asn Asp  Tyr His His Ala Ile  Asp Ala Tyr Leu Ser  Ala Ile Cys 
    1010                 1015                 1020             


Gly Asn  Leu Leu Tyr Gln Asn  Tyr Pro Asn Leu Arg  Pro Phe Phe 
    1025                 1030                 1035             


Val Tyr  Gly Gln Tyr Lys Lys  Phe Ser Ser Asp Pro  Asp Lys Glu 
    1040                 1045                 1050             


Lys Ala  Ile Phe Asn Lys Thr  Arg Lys Phe Ser Phe  Ile Ser Gln 
    1055                 1060                 1065             


Leu Leu  Lys Asn Lys Ser Glu  Asn Ser Lys Glu Ile  Ala Lys Lys 
    1070                 1075                 1080             


Leu Lys  Arg Ala Tyr Gln Phe  Lys Tyr Met Leu Val  Ser Arg Glu 
    1085                 1090                 1095             


Thr Glu  Thr Arg Asp Gln Glu  Met Phe Lys Met Thr  Val Tyr Pro 
    1100                 1105                 1110             


Arg Phe  Ser His Asp Thr Val  Lys Ala Pro Arg Asn  Leu Ile Pro 
    1115                 1120                 1125             


Lys Lys  Met Gly Met Ser Pro  Asp Ile Tyr Gly Gly  Tyr Thr Asn 
    1130                 1135                 1140             


Asn Ser  Asp Ala Tyr Met Val  Ile Val Arg Ile Asp  Lys Lys Lys 
    1145                 1150                 1155             


Gly Thr  Glu Tyr Lys Ile Leu  Gly Ile Pro Thr Arg  Glu Leu Val 
    1160                 1165                 1170             


Asn Leu  Lys Lys Ala Glu Lys  Glu Asp His Tyr Lys  Ser Tyr Leu 
    1175                 1180                 1185             


Lys Glu  Ile Leu Thr Pro Arg  Ile Leu Tyr Asn Lys  Asn Gly Lys 
    1190                 1195                 1200             


Arg Asp  Lys Lys Ile Thr Ser  Phe Glu Ile Val Lys  Ser Lys Ile 
    1205                 1210                 1215             


Pro Tyr  Lys Gln Val Ile Gln  Asp Gly Asp Lys Lys  Phe Met Leu 
    1220                 1225                 1230             


Gly Ser  Ser Thr Tyr Val Tyr  Asn Ala Lys Gln Leu  Thr Leu Ser 
    1235                 1240                 1245             


Thr Glu  Ser Met Lys Ala Ile  Thr Asn Asn Phe Asp  Lys Asp Ser 
    1250                 1255                 1260             


Asp Glu  Asn Asp Ala Leu Ile  Lys Ala Tyr Asp Glu  Ile Leu Asp 
    1265                 1270                 1275             


Lys Val  Asp Lys Tyr Leu Pro  Leu Phe Asp Ile Asn  Lys Phe Arg 
    1280                 1285                 1290             


Glu Lys  Leu His Ser Gly Arg  Glu Lys Phe Ile Lys  Leu Ser Leu 
    1295                 1300                 1305             


Glu Asp  Lys Lys Asp Thr Ile  Leu Lys Val Leu Glu  Gly Leu His 
    1310                 1315                 1320             


Asp Asn  Ala Val Met Thr Lys  Ile Pro Thr Ile Gly  Leu Ser Thr 
    1325                 1330                 1335             


Pro Leu  Gly Phe Met Gln Phe  Pro Asn Gly Val Ile  Leu Ser Glu 
    1340                 1345                 1350             


Asn Ala  Lys Leu Ile Tyr Gln  Ser Pro Thr Gly Leu  Phe Lys Lys 
    1355                 1360                 1365             


Ser Val  Lys Ile Ser Asp Leu  
    1370                 1375 


<210>  1069
<211>  1376
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1069

Met Ser Asn Phe Asp Asn His Lys Asp Ser Ile Cys Lys His Lys Gly 
1               5                   10                  15      


Leu Lys Lys Gly Asn Tyr Tyr Ile Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Ala Thr Asn Glu Gln Tyr Glu Leu Leu Lys Phe Lys 
        35                  40                  45              


Ser His Lys Met Trp Gly Ser Arg Leu Phe Ala Glu Ala Gln Thr Ala 
    50                  55                  60                  


Val Asp Thr Arg Met Lys Arg Ser Ala Arg Arg Arg Leu Gln Arg Arg 
65                  70                  75                  80  


Lys Phe Arg Leu Ile Leu Leu Glu Ser Leu Phe Ser Lys Glu Ile Asn 
                85                  90                  95      


Ala Val Asp Glu Thr Phe Phe Met Arg Leu His Glu Ser Asn Tyr His 
            100                 105                 110         


Val Glu Asp Lys Thr Asn Leu Val Lys Tyr Thr Leu Phe Ile Asp Lys 
        115                 120                 125             


Asn Tyr Thr Asp Val Asp Tyr Tyr Lys Glu Phe Pro Thr Ile Tyr His 
    130                 135                 140                 


Leu Arg His His Leu Met Thr Glu Gly Thr Lys Asp Ile Arg Leu Leu 
145                 150                 155                 160 


Phe Leu Ala Ile His His Ile Leu Lys Tyr Arg Gly Asn Phe Leu Tyr 
                165                 170                 175     


Gly Lys Lys Glu Phe Ser Val Asp Gly Glu Met Tyr Ser Val Leu Arg 
            180                 185                 190         


Ser Thr Leu Glu Glu Leu Gly Phe Gly Ser Tyr Val Glu Asp Glu Asn 
        195                 200                 205             


Val Leu Gln Ser Ile Ala Asn Leu Val Leu Asp Arg Gly Ile Ala Arg 
    210                 215                 220                 


Ser Glu Lys Val Lys Gln Ile Glu Pro Leu Ile Ala Gln Gly Lys Lys 
225                 230                 235                 240 


Leu Ser Ser Val Glu Lys Asn Arg Leu Lys Ala Trp Ala Tyr Leu Ile 
                245                 250                 255     


Met Gly Leu Lys Ala Asn Leu Val Asn Leu Phe Thr Asp Glu Asp Val 
            260                 265                 270         


Glu Val Val Leu Pro Glu Glu Lys Glu Phe Lys Ser Ile Asn Leu His 
        275                 280                 285             


Glu Gly Ile Tyr Glu Asp Val Arg Asp Ala Tyr Glu Asp Ala Trp Gly 
    290                 295                 300                 


Asp Arg Phe Ala Ile Val Asp Gln Cys Lys Val Leu His Asp Ile Ile 
305                 310                 315                 320 


Val Ile Ser Ser Ile Ile Lys Pro Gly Gln Ser Leu Ser Glu Ala Lys 
                325                 330                 335     


Ile Glu Ser Tyr Asn Gln His Lys Glu Asp Leu Val Leu Leu Lys Ser 
            340                 345                 350         


Val Leu Lys Glu Asp Lys Lys Leu Tyr Asp Asp Met Phe Val Val Asp 
        355                 360                 365             


Ile Asp Lys Gly Thr Asn Tyr Val Lys Tyr Ile Lys Gln Gly Val Asn 
    370                 375                 380                 


Gly Asn Thr Ser Thr Thr Gln Glu Ala Phe Tyr Lys Tyr Val Arg Lys 
385                 390                 395                 400 


Val Val Ser Val Leu Pro Glu Thr Glu Asn Thr Arg Cys Ile Val Gln 
                405                 410                 415     


Lys Leu Glu Glu Gly Thr Phe Leu Pro Leu Gln Arg Ile Ser Lys Asn 
            420                 425                 430         


Gly Val Val Pro Tyr Gln Leu His Lys Glu Glu Leu Val Arg Ile Leu 
        435                 440                 445             


Glu Lys Ala Lys Leu Asn Phe Pro Phe Leu Asn Glu Val Glu Asp Gly 
    450                 455                 460                 


Leu Thr Val Ala Asp Lys Ile Ile Ser Ile Leu Glu Phe Lys Ile Pro 
465                 470                 475                 480 


Tyr Tyr Val Gly Pro Leu Asn Pro Thr His Ser Ile Glu Asn Gly Gly 
                485                 490                 495     


Phe Ala Trp Val Val Arg Lys Glu Ala Gly Gln Val Leu Pro Trp Asn 
            500                 505                 510         


Phe Glu Gln Lys Val Asp Val Gln Ala Ser Ala Val Lys Phe Ile Glu 
        515                 520                 525             


Asn Leu Thr Asn Lys Cys Thr Tyr Leu Ile Asp Gln Asp Val Leu Pro 
    530                 535                 540                 


Lys Tyr Ser Leu Leu Tyr Ala Glu Phe Ser Leu Leu Asn Glu Leu Asn 
545                 550                 555                 560 


Asn Ile Arg Val Asn Asp Lys Pro Leu Pro Ala Asp Val Lys Gln Ala 
                565                 570                 575     


Phe Ile Arg Asp His Phe Ile Ser Leu Gln Asp Ser Lys Thr Ala Thr 
            580                 585                 590         


Lys Lys Val Val Ala Lys Tyr Leu Val Asp Asn Asn Tyr Val Ser Gln 
        595                 600                 605             


Pro Pro Ile Ile Thr Gly Met Asp Gln Gln Ile Ala Thr Lys Leu Thr 
    610                 615                 620                 


Ser His Arg Asp Met Thr Arg Ile Leu Gly Glu His Phe Asp Tyr Ala 
625                 630                 635                 640 


Met Ala Glu Asp Ile Ile Arg Tyr Ile Thr Ile Phe Gly Glu Ser Lys 
                645                 650                 655     


Asp Met Leu Arg Gln Val Leu Lys Asn Tyr Tyr Gly Asp Arg Leu Thr 
            660                 665                 670         


Thr Glu Gln Ile Lys Lys Leu Gly Lys Leu Thr Tyr Thr Gly Trp Gly 
        675                 680                 685             


Arg Leu Ser Lys Lys Phe Leu Ser Asp Ile Trp Gly Met Lys Lys Asn 
    690                 695                 700                 


Asp Asp Asp Ala Glu Ala Gly Thr Ile Ile Glu His Met Arg Glu Gly 
705                 710                 715                 720 


Gln Glu Asn Leu Met Gln Leu Leu Ser Ser Ser Tyr Ser Phe Ile Asp 
                725                 730                 735     


Val Val Gln Glu Tyr Val Thr Asn Val Leu Gly Ala Lys Glu Lys Thr 
            740                 745                 750         


Ala Phe Glu Met Leu Glu Asp Leu Ser Leu Ser Pro Met Val Lys Arg 
        755                 760                 765             


Ser Val Trp Gln Thr Leu Arg Ile Leu Asp Glu Ile Val Ser Ile Arg 
    770                 775                 780                 


Lys Glu Leu Pro Lys Lys Ile Phe Val Glu Val Ala Arg Thr Asn Lys 
785                 790                 795                 800 


Ala Asp Lys Lys Arg Thr Gln Ser Arg Lys Ala Leu Leu Glu Glu Leu 
                805                 810                 815     


Tyr Glu Gln Ile Lys Asp Glu Asp Val His Val Leu Gln Gln Glu Leu 
            820                 825                 830         


Glu Ser Lys Glu Asp Ser Ala Leu Asn Ser Lys Lys Leu Tyr Leu Tyr 
        835                 840                 845             


Phe Thr Gln Met Gly Arg Cys Met Tyr Ser Gly Arg Arg Ile Asp Ile 
    850                 855                 860                 


His Glu Leu Phe Thr Asp Thr Tyr Asp Ile Asp His Ile Tyr Pro Arg 
865                 870                 875                 880 


Ser Leu Thr Lys Asp Asp Ser Trp His Asn Ile Val Leu Val Glu Lys 
                885                 890                 895     


Gln Leu Asn Ala Glu Lys Thr Asp Arg Phe Pro Ile Leu Ser Ala Ile 
            900                 905                 910         


Gln Ser Lys Gln Lys Gly Phe Trp Lys Leu Leu Trp Asp Arg Gly Phe 
        915                 920                 925             


Ile Ser Glu Lys Lys Tyr Asp Arg Leu Val Arg Thr Thr Asp Leu Thr 
    930                 935                 940                 


Asp Glu Glu Leu Asn Gln Phe Ile Asn Arg Gln Ile Val Ser Thr Asn 
945                 950                 955                 960 


Gln Ser Val Lys Ala Val Thr Thr Leu Leu His Gln Leu Tyr Pro Glu 
                965                 970                 975     


Thr Glu Val Val Phe Ser Lys Ala Glu Asn Ile Ser Asp Phe Arg Arg 
            980                 985                 990         


Asp Tyr Gly Phe Val Lys Val Arg  Ser Ile Asn His His  His His Ala 
        995                 1000                 1005             


Lys Asp  Ala Tyr Leu Asn Ile  Val Val Gly Asn Val  Tyr His Glu 
    1010                 1015                 1020             


Lys Phe  Thr Lys Asn Phe Leu  Ser Phe Ala Lys Gln  Lys Gly Thr 
    1025                 1030                 1035             


His Arg  Thr Tyr Asn Leu Thr  Lys Met Phe Glu Tyr  Glu Ile Lys 
    1040                 1045                 1050             


Leu Thr  Glu Glu Ser Lys Lys  Val Ile Trp Asp Pro  Asn Ile His 
    1055                 1060                 1065             


Met Asp  Ile Val Lys Lys Met  Met Lys Ser Asn Asp  Val Arg Val 
    1070                 1075                 1080             


Thr Gln  Arg Ala Val Pro Gln  Lys Gly Gly Leu Ser  Asp Glu Thr 
    1085                 1090                 1095             


Ile Tyr  Lys Lys Gly Ile Ala  Lys Arg Gly Val Tyr  Leu Pro Leu 
    1100                 1105                 1110             


Lys Ser  Ser Asp Glu Arg Val  Arg Asp Val Glu Lys  Tyr Gly Gly 
    1115                 1120                 1125             


Arg Thr  Ser Ile Thr Ile Ser  Glu Tyr Ile Ile Leu  Asn Ile Arg 
    1130                 1135                 1140             


Asp Thr  Ser Asp Gln Asp Arg  Ile Glu Ile Phe Ser  Leu Pro Leu 
    1145                 1150                 1155             


Tyr Ile  Ala Gly Lys Lys Asp  Ile His Lys Ala Ile  Glu Glu Ala 
    1160                 1165                 1170             


Tyr Leu  Ser Thr Cys Lys Lys  Pro Ala Ser Val Val  Asp Ile Gln 
    1175                 1180                 1185             


Ile Leu  Tyr Arg Gly Leu Tyr  Ile Gly Ser Lys Val  Asn Ile Asn 
    1190                 1195                 1200             


Gly Phe  Thr Tyr Tyr Met Gly  Gly Lys Thr Gly Ser  Asn Val Ser 
    1205                 1210                 1215             


Val Asp  Ser Ala Val Asp Val  Val Met Asp Ile Thr  Ser Ser Val 
    1220                 1225                 1230             


Tyr Ile  Lys Leu Leu Glu Lys  Val Ala Ile Lys Lys  Ile Glu Asn 
    1235                 1240                 1245             


Ser Lys  Thr Phe Arg Ile Glu  Asn Ile Lys Thr Ser  Tyr Asn His 
    1250                 1255                 1260             


Lys Glu  Val Thr Val Ser Lys  Glu Gln Asn Ile Ala  Leu Tyr Gln 
    1265                 1270                 1275             


Glu Leu  Leu Lys Lys Leu Asp  Thr Pro Leu Phe Ser  Lys Met Lys 
    1280                 1285                 1290             


Gly Asn  Lys Leu Glu Glu Phe  Arg Thr Val Gly Phe  Asp Thr Phe 
    1295                 1300                 1305             


Ile Ala  Leu Ser Leu Glu Asp  Gln Ala Ser Val Leu  Leu Glu Val 
    1310                 1315                 1320             


Leu Asn  Val Leu Thr Asn Phe  Lys Thr Lys Arg Asp  Ile Thr Lys 
    1325                 1330                 1335             


Ile Gly  Met Thr Ser Ser Arg  Ser Thr Leu Gly Ile  Asn Ile Ser 
    1340                 1345                 1350             


Asn Thr  Glu Gln Phe Ser Val  Ile Thr Thr Ser Ile  Thr Gly Leu 
    1355                 1360                 1365             


Tyr Glu  Gln Glu Ile Lys Ile  Lys 
    1370                 1375     


<210>  1070
<211>  1376
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1070

Met Thr Thr Lys Asn Arg Glu Lys Asn Asn Lys Phe His Lys Ala Ser 
1               5                   10                  15      


Pro Leu Tyr Thr Leu Gly Leu Asp Ile Gly Thr Thr Ser Val Gly Phe 
            20                  25                  30          


Thr Ala Ile Asp Glu Ala Gln Arg Pro Met Arg Ala Lys Gly Lys Thr 
        35                  40                  45              


Val Ile Gly Val Arg Leu Phe Gln Glu Gly Gln Ser Ala Val Gly Arg 
    50                  55                  60                  


Lys Asn Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg Arg Arg Arg Arg 
65                  70                  75                  80  


Leu Asn Leu Leu Lys Gln Phe Phe Ser Ala Pro Leu Arg Lys Ile Asp 
                85                  90                  95      


Pro Thr Phe Phe Lys Arg Leu Lys Ala Ser Ser Leu Ser Pro Ala Asp 
            100                 105                 110         


Lys Arg Arg Tyr Leu Ala Pro Gly Leu Phe Pro Asn Gly Gly Asp Ile 
        115                 120                 125             


Lys Phe Tyr Gln Lys Tyr Pro Thr Ile Tyr His Leu Arg Leu Ala Leu 
    130                 135                 140                 


Met Thr Glu Lys Arg Pro Phe Asp Leu Arg Glu Val Tyr Leu Ala Ile 
145                 150                 155                 160 


His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn Pro Leu Pro Val 
                165                 170                 175     


Ser Ala Phe Arg Gly Arg Asn Gln Arg Phe Glu Asp Gln Phe Ser Glu 
            180                 185                 190         


Leu Asn Gln Leu Tyr Lys Ser Leu Glu Gly Glu Ser Pro Phe Gln Phe 
        195                 200                 205             


Asp Glu Lys Ile Phe Ser Glu Leu Asp Lys Gln Leu Leu Val Asn Asp 
    210                 215                 220                 


Gly Arg Lys Ile Asp Arg Gln Lys Gln Leu Ala Lys Arg Val Phe Ile 
225                 230                 235                 240 


Lys Thr Asn Glu Lys Arg Ile Asp Ser His His Gln Lys Ile Ala Ser 
                245                 250                 255     


Ala Phe Ser Lys Ala Val Phe Gly Tyr Lys Phe Arg Pro Asp Val Leu 
            260                 265                 270         


Leu Asn Ser Asp Pro Asn Glu Ser Ile Asp Glu Phe Ile Lys Phe Gly 
        275                 280                 285             


Asp Glu Asp Phe Asp Asp Lys Leu Val Ile Leu Arg Glu Ser Leu Asn 
    290                 295                 300                 


Ala Thr Gln Lys Glu Ile Leu Ala Val Thr Gln Arg Ile Tyr Ser Gln 
305                 310                 315                 320 


Ile Ile Leu Asn Glu Ile Val Pro Ser Arg Glu Thr Leu Ser Glu Ala 
                325                 330                 335     


Met Val Ser Arg Tyr Gln Lys His His Ser Asp Leu Gln Leu Leu Thr 
            340                 345                 350         


Glu Ala Cys Lys Lys Met Thr Lys Ala Lys Ala Ala Arg Ile Gln Ala 
        355                 360                 365             


Ala Tyr Thr Asp Tyr Ile Gly Lys Gly Asp Gly His Lys Leu Val His 
    370                 375                 380                 


Asp Gln Phe Val Ala Leu Ile Lys Lys Asn Leu Asp Pro Ser Glu Glu 
385                 390                 395                 400 


Gly Arg Asp Leu Ala Glu Arg Leu Ala Gln Asp Asp Phe Met Pro Leu 
                405                 410                 415     


Gln Arg Thr Pro Leu Asn Arg Val Ile Pro His Gln Leu Gln Gln Glu 
            420                 425                 430         


Glu Leu Asp Arg Ile Ile Glu Asn Gln Gly Glu Tyr Tyr Pro Phe Leu 
        435                 440                 445             


Lys Thr Leu Asn Pro Asn Arg Ser Arg Ala Gly Val Ala Asn Tyr Gln 
    450                 455                 460                 


Leu Asp Glu Leu Val Ser Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu 
465                 470                 475                 480 


Ile Thr Ala Thr Asp Gln Leu His Thr Ser Gly Ala Lys Phe Ala Trp 
                485                 490                 495     


Met Thr Arg Arg Ala Ala Gly Glu Ile Thr Pro Trp Asn Phe Asp Gln 
            500                 505                 510         


Lys Val Asp Arg Leu Ala Ser Ala Asn Gln Phe Ile Arg Gln Asn Met 
        515                 520                 525             


Pro Lys Asp Asn His Leu Leu Glu Ala Asp Val Leu Pro Ala Asn Ser 
    530                 535                 540                 


Leu Thr Tyr Gln Arg Tyr Leu Val Leu Asp Glu Leu Asn Ala Val Lys 
545                 550                 555                 560 


Val Asn His Lys Ser Leu Thr Val Ser Gln Lys Gln Arg Ile Phe Asn 
                565                 570                 575     


Asp Leu Phe Lys Arg Gln Arg Thr Val Thr Val Leu Asn Leu Gln Asn 
            580                 585                 590         


Tyr Leu Lys Ser Ile Glu Lys Leu Pro Thr Leu Pro Val Ile Thr Gly 
        595                 600                 605             


Val Ser Asp Ser Glu Arg Phe Lys Thr Thr Leu Ser Thr Tyr Leu Asp 
    610                 615                 620                 


Phe Lys Asn Ile Phe Gly Gly Lys Val Asp Asp Ser Ser Gln Lys Glu 
625                 630                 635                 640 


Lys Phe Glu Lys Ile Ile Leu Tyr Ala Thr Ile Phe Glu Asp His Ala 
                645                 650                 655     


Val Tyr Lys Ala Lys Leu Asp Gln Ile Asp Trp Leu Gly Gln Gln Gln 
            660                 665                 670         


Lys Thr Asp Leu Leu Ser Lys Asn Tyr Gln Gly Trp Gly Gln Leu Ser 
        675                 680                 685             


Glu Lys Leu Leu Leu Gly Leu Lys Asn Gln Ala Gly Gln Ser Ile Leu 
    690                 695                 700                 


Asp Glu Leu Trp Gln Thr Asn Lys Arg Phe Arg Glu Ile Met Asn Glu 
705                 710                 715                 720 


Ala His Phe Ala Lys Gln Ile Met Ala Ser Asn Ala Ala Leu Leu Arg 
                725                 730                 735     


Lys Lys Gly Leu Asp Asp Val Leu Ser Thr Ala Phe Leu Ser Pro Gln 
            740                 745                 750         


Asn Lys Lys Ala Ile Arg Gln Thr Val Glu Val Val Asp Asp Ile Val 
        755                 760                 765             


Lys Ala Ile Gly Cys Pro Pro Gln Lys Ile Ser Leu His Phe Ser Lys 
    770                 775                 780                 


Ala Pro Ala Asp Ala Ile Arg His Glu Arg Asp Asn Ala Asn Ile Ile 
785                 790                 795                 800 


Leu Arg Thr Tyr Arg Asn Leu Pro Ala Lys Met Val Asp Asp Gln Leu 
                805                 810                 815     


Lys Ala Glu Leu Gln Ala Thr Ala Glu Lys His Gln Val Leu Ser Asp 
            820                 825                 830         


Lys Leu Tyr Leu Tyr Phe Thr Gln Leu Gly Arg Asp Leu Tyr Thr Gly 
        835                 840                 845             


Glu Met Leu Lys Met Ser Glu Leu Glu Asn Asp Met Ile Asp His Ile 
    850                 855                 860                 


Met Pro Gln Thr Phe Ala Tyr Asp Asp Ser Val Asn Asn Arg Val Leu 
865                 870                 875                 880 


Val Ser Gln Asn His Ser Ser Gln Leu Gly Lys Thr Ser Gly Glu Lys 
                885                 890                 895     


Ser Asp Ala Lys Met Ile Arg Phe Trp Gln Val Leu Arg Asp Asn Lys 
            900                 905                 910         


Leu Ile Ser Glu Gln Lys Tyr Gln Asn Leu Thr Leu Asp Ser Asp His 
        915                 920                 925             


Leu Ser Lys Ser Thr Arg Glu Gln Leu Leu Asn Arg Tyr Leu Val Glu 
    930                 935                 940                 


Lys Asn Pro Ile Thr Gln Trp Val Ala Ser Val Leu Thr Glu Lys Tyr 
945                 950                 955                 960 


Gly Asp Asn Gly Thr Arg Val Leu Thr Ile Asn Pro Arg Met Val Tyr 
                965                 970                 975     


Gln Met Arg Arg Gln Phe Asn Leu Val Asn Leu Pro Glu Val Asn Asp 
            980                 985                 990         


Tyr Asp His Gly Leu Asp Ala Tyr  Leu Ala Ala Phe Ile  Gly Asn Tyr 
        995                 1000                 1005             


Val Asp  Arg Arg Tyr Pro Arg  Leu Arg Ser Tyr Phe  Val Tyr Gly 
    1010                 1015                 1020             


Asp Phe  Ser Ser Ser Lys Glu  Ile Val Phe Arg Leu  Asn Arg Phe 
    1025                 1030                 1035             


Asn Phe  Leu Phe Asp Leu Gly  Gln Ala Glu Pro Asn  Glu Leu Thr 
    1040                 1045                 1050             


Asp Trp  Glu Thr Gly Glu Met  Ile Trp Asp Lys Ala  Ala Ala Asn 
    1055                 1060                 1065             


Ser Val  Leu Arg Asp Val Tyr  Gln Tyr Lys Phe Met  Leu Ile Ser 
    1070                 1075                 1080             


Gln Ala  Val Tyr Ser Arg Gln  Gly Ala Met Phe Asn  Gln Thr Ile 
    1085                 1090                 1095             


Tyr Pro  Ala Ser Gln Ala Ala  Gly Arg Gln Leu Ile  Pro Val Lys 
    1100                 1105                 1110             


Lys Asn  Arg Ala Thr Lys Leu  Tyr Gly Gly Tyr Ser  Gly Asn Val 
    1115                 1120                 1125             


Asp Ala  Tyr Leu Ala Ile Val  Arg Leu Gly Gly Pro  Lys Ser Asp 
    1130                 1135                 1140             


Gln Tyr  Arg Val Val Gly Ile  Pro Thr Arg Met Val  Gly Arg Leu 
    1145                 1150                 1155             


Gln Ser  Ala Met Lys Gln Gly  Ala Asp Thr Tyr Ala  Asn Thr Leu 
    1160                 1165                 1170             


His Lys  Ile Leu Gln Pro Gln  Phe Thr Lys Arg Lys  Lys Asn Arg 
    1175                 1180                 1185             


Gln Thr  Gly Tyr Tyr Glu Glu  Thr Thr Glu Pro Phe  Ala Val Ile 
    1190                 1195                 1200             


Leu Asp  Lys Val Tyr Tyr Arg  Gln Leu Ile Ile Asp  Gly Gln Gln 
    1205                 1210                 1215             


Lys Phe  Met Leu Gly Ser Ser  Thr Tyr Gln Phe Asn  Ala Lys Gln 
    1220                 1225                 1230             


Leu Val  Leu Ser Ser Arg Ala  Arg Gln Ile Leu Ser  Gly Glu Glu 
    1235                 1240                 1245             


Arg Leu  Thr Asp Glu Asn Gly  Ser Gln Asp Leu Ile  Asp Val Tyr 
    1250                 1255                 1260             


Asp Glu  Ile Leu Thr Lys Ile  Asp Arg Tyr Phe Glu  Leu Tyr Asp 
    1265                 1270                 1275             


Gln Arg  His Phe Arg Gln Ser  Leu His Asp Gly Arg  Lys Gln Phe 
    1280                 1285                 1290             


Ile Ser  Leu Pro Ile His Asn  Val Tyr Arg Ala Asn  Arg Leu Ile 
    1295                 1300                 1305             


Glu His  Gly Lys Tyr Glu Thr  Leu Ser Gln Ile Leu  Asn Gly Leu 
    1310                 1315                 1320             


His Ala  Asn Thr Met Met Thr  Asn Leu Arg Tyr Leu  Gly Ile Ser 
    1325                 1330                 1335             


Ser Pro  Phe Gly Lys Phe Gln  Ser Pro Asn Gly Ile  Lys Leu Ser 
    1340                 1345                 1350             


Lys Asp  Ala Val Leu Val Tyr  Gln Ser Pro Ser Gly  Leu Phe Glu 
    1355                 1360                 1365             


Arg Arg  Val Arg Leu Arg Asp  Leu 
    1370                 1375     


<210>  1071
<211>  1376
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1071

Met Asn Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Thr Glu Glu Met Ser Lys Val Asp Ile 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Asn Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Ala Asp Phe Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Asn Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Val Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Asp Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu 
                325                 330                 335     


Lys Gln Phe Ile Lys Thr Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Leu 
    370                 375                 380                 


Glu Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Ile Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Gln Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Lys Ala Arg Ser Ala Glu Asp Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Gln Gln Ile Val Thr Gln Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Gln Tyr Leu His Asn Val Asp Ser Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             


Asp Ser Lys Asn Glu Ala Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala His Tyr Ala 
625                 630                 635                 640 


Ser Ile Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Tyr Asp 
            660                 665                 670         


Lys Gln Ser Lys Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Glu 
        675                 680                 685             


Ile Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 


Lys Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asn Asp Val 
705                 710                 715                 720 


Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Leu Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Ala Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ala Leu Lys Asn Leu Ala His Gly Leu Asp Ser Asn Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Lys Ser Leu Asp 
            820                 825                 830         


Ile Asn Gln Leu Ser Ser Cys Asp Ile Asp His Ile Ile Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Ile Val Gln 
865                 870                 875                 880 


Lys Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Glu Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp 
            900                 905                 910         


Glu Arg Asp Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr 
    930                 935                 940                 


Glu Val Thr Glu Lys Asp Lys Lys Asp Arg Ser Val Lys Ile Ile Thr 
945                 950                 955                 960 


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Arg Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Pro Tyr Leu 
            980                 985                 990         


Asn Ala Val Val Ala Lys Ala Ile  Leu Lys Lys Tyr Pro  Lys Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr Gln Lys Tyr Asp  Leu Lys Arg 
    1010                 1015                 1020             


Tyr Ile  Ser Arg Thr Lys Asp  Pro Lys Glu Val Glu  Lys Ala Thr 
    1025                 1030                 1035             


Glu Lys  Tyr Phe Phe Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Glu 
    1040                 1045                 1050             


Glu Val  His Tyr Ala Asp Gly  Thr Ile Val Lys Arg  Glu Asn Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Lys Glu Lys 
    1070                 1075                 1080             


Asp Phe  Ala Thr Ile Lys Lys  Val Leu Ser Leu Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Thr Glu Glu  Gln Thr Val Gly Gln  Asn Gly Gly 
    1100                 1105                 1110             


Leu Phe  Asp Asn Asn Ile Val  Ser Lys Lys Lys Val  Val Asp Ala 
    1115                 1120                 1125             


Ser Lys  Leu Thr Pro Ile Lys  Ser Gly Leu Ser Pro  Glu Lys Tyr 
    1130                 1135                 1140             


Gly Gly  Tyr Ala Arg Pro Thr  Ile Ala Tyr Ser Val  Leu Val Ile 
    1145                 1150                 1155             


Ala Asp  Ile Glu Lys Gly Lys  Ala Lys Lys Leu Lys  Arg Ile Lys 
    1160                 1165                 1170             


Glu Met  Val Gly Ile Thr Val  Gln Asp Lys Lys Lys  Phe Glu Ala 
    1175                 1180                 1185             


Asn Pro  Ile Ala Tyr Leu Glu  Glu Cys Gly Tyr Lys  Asn Ile Asn 
    1190                 1195                 1200             


Pro Asn  Leu Ile Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Phe 
    1205                 1210                 1215             


Asn Asn  Gly Gln Arg Arg Leu  Leu Ala Ser Ser Ile  Glu Leu Gln 
    1220                 1225                 1230             


Lys Gly  Asn Glu Leu Ile Val  Pro Tyr His Phe Thr  Ala Leu Leu 
    1235                 1240                 1245             


Tyr His  Ala Gln Arg Ile Asn  Lys Ile Ser Glu Pro  Ile His Lys 
    1250                 1255                 1260             


Gln Tyr  Val Glu Thr His Gln  Ser Glu Phe Lys Glu  Leu Leu Thr 
    1265                 1270                 1275             


Ala Ile  Ile Ser Leu Ser Lys  Lys Tyr Ile Gln Lys  Pro Asn Val 
    1280                 1285                 1290             


Glu Ser  Leu Leu Gln Gln Ala  Phe Asp Gln Ser Asp  Lys Asp Ile 
    1295                 1300                 1305             


Tyr Gln  Leu Ser Glu Ser Phe  Ile Ser Leu Leu Lys  Leu Ile Ser 
    1310                 1315                 1320             


Phe Gly  Ala Pro Gly Thr Phe  Lys Phe Leu Gly Val  Glu Ile Ser 
    1325                 1330                 1335             


Gln Ser  Asn Val Arg Tyr Gln  Ser Val Ser Ser Cys  Phe Asn Ala 
    1340                 1345                 1350             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1355                 1360                 1365             


Asp Leu  Ser Lys Leu Gly Glu  Asp 
    1370                 1375     


<210>  1072
<211>  1376
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1072

Met Asp Lys Lys Tyr Gly Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile 
1               5                   10                  15      


Gly Trp Thr Val Val Asp Lys Tyr Gly His Ile Ile Arg Val Lys Gly 
            20                  25                  30          


Gln Thr Gly Ile Gly Val Arg Leu Phe Arg Glu Gly Gln Thr Ala Ala 
        35                  40                  45              


Glu Arg Arg Thr Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg Arg 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Arg Glu Ile Phe Asp Glu Pro Ile Ser Ala 
65                  70                  75                  80  


Ile Asp Glu Asn Phe Phe Ala Arg Gln Lys Ala Ser Ser Leu Ala Pro 
                85                  90                  95      


Gly Asp Ala Arg Tyr Gln Gln Ala Phe Gln Leu Phe Asn Asp Arg Thr 
            100                 105                 110         


Asp Gln Ala Phe Tyr Cys Lys Tyr Pro Thr Ile Tyr His Leu Arg Gln 
        115                 120                 125             


Ala Leu Met Thr Glu Ser Arg Gln Phe Asp Ile Arg Glu Ile Tyr Leu 
    130                 135                 140                 


Ala Leu His Gln Ile Ile Lys Tyr Arg Gly His Phe Leu Leu Gly Gly 
145                 150                 155                 160 


Ala Ala Lys Asp Phe Gln Ser Asp Lys Leu Asp Leu Glu Ala Tyr Phe 
                165                 170                 175     


Thr Thr Ile Asn Ala Gln Leu Ala Gly Ile Tyr Ser Asp Gly Ser Val 
            180                 185                 190         


Gln Leu Pro Thr Leu Asp Trp Asn Gln Ala Cys Gln Glu Leu Thr Asp 
        195                 200                 205             


Thr Ser Arg Thr Gln Ser Asp Arg Lys Asn Trp Leu Val Lys Glu Leu 
    210                 215                 220                 


Lys Ser Tyr Gly Asp Ser Gln Leu Lys Pro Ile Tyr Thr Gln Ile Ala 
225                 230                 235                 240 


Asn Ala Ile Leu Gly Phe Lys Thr Asp Leu Ala Lys Ile Met Gly Ile 
                245                 250                 255     


Asn Asp Pro Glu Lys Ala Leu Ser Phe Asn Leu Asp Ala Leu Asp Asp 
            260                 265                 270         


His Arg Asp Asp Val Ala Glu Leu Leu Thr Asp Glu Gln Ile Pro Leu 
        275                 280                 285             


Val Asn Leu Leu Ala Lys Leu His Thr Ala Leu Glu Leu Ala Ala Ile 
    290                 295                 300                 


Leu Pro Asp Gly Gln Thr Leu Ser Gly Lys Met Val Glu Ile Tyr Glu 
305                 310                 315                 320 


Thr His Arg Lys Gln Leu Lys Thr Tyr Lys Ala Phe Ile Arg Gln Leu 
                325                 330                 335     


Pro Asp Arg Gln Gln Arg Leu Ala Leu Lys Gln Ala Tyr Asp His Tyr 
            340                 345                 350         


Ile Asp Gln Gly Leu Asn Arg Glu Ala Phe Tyr Ser Glu Val Lys Ala 
        355                 360                 365             


Ile Leu Glu Lys Gln Lys Pro Leu Thr Ala Thr Gly Gln Ala Met Lys 
    370                 375                 380                 


Lys Ala Met Asp Gln Asn Ala Phe Leu Leu Lys Gln Arg Thr Lys Thr 
385                 390                 395                 400 


Asn Ile Ala Ile Pro Tyr Gln Val Gln Gln Gln Glu Met Asp Gln Ile 
                405                 410                 415     


Ile Ala Asn Gln Ser Lys Tyr Tyr Pro Trp Leu Ala Thr Pro Asn Pro 
            420                 425                 430         


Val Lys Ser Arg Arg Val Ile Ala Pro Tyr Lys Leu Asp Glu Leu Val 
        435                 440                 445             


Cys Phe Arg Val Asn Tyr Tyr Val Gly Pro Leu Val Gln Ser Lys Pro 
    450                 455                 460                 


Gly Glu Ala Thr Lys Glu Ala Lys Phe Ala Trp Met Thr Arg Lys Asp 
465                 470                 475                 480 


Pro Gln Ser Thr Val Ala Ile Thr Pro Trp Asn Phe Asp Gln Gln Val 
                485                 490                 495     


Asp Arg Gln Ala Ser Ala Asn Ala Phe Ile Gln Arg Met Lys Thr Thr 
            500                 505                 510         


Asp Thr Tyr Leu Leu Gly Glu Asp Val Ile Pro Lys Asn Ser Leu Leu 
        515                 520                 525             


Tyr Gln Lys Tyr Glu Val Leu Asn Glu Leu Asn Asn Val Arg Val Asn 
    530                 535                 540                 


Gly Asp Trp Leu Asp Pro Gln Arg Lys Gln Tyr Leu Phe Lys Lys Leu 
545                 550                 555                 560 


Phe Met Asp Gln Pro Gln Ser Val Val Thr Val Lys Ala Leu Gln Lys 
                565                 570                 575     


Thr Leu Val Val Ile Gly Ala Cys Pro Thr Leu Pro Thr Val Glu Gly 
            580                 585                 590         


Leu Ala Asn Pro Lys Arg Phe Leu Ser Gln Leu Thr Thr Tyr His Asp 
        595                 600                 605             


Phe Cys Lys Leu Leu Pro Asp Asp Ile Gln Arg Ala Asp Arg Gln Ala 
    610                 615                 620                 


Asp Ile Glu Lys Ile Ile Asn Trp Ser Thr Ile Phe Glu Asp Thr Ala 
625                 630                 635                 640 


Ile Phe Lys Glu Lys Leu Arg Asp Ile Lys Trp Leu Ser Ala Asp Gln 
                645                 650                 655     


Ile Arg Arg Leu Ser Ser Arg Pro Arg Tyr Arg Gly Trp Gly Arg Phe 
            660                 665                 670         


Ser Ala Lys Leu Leu Cys Gly Leu His Asp Arg Gln Gly Asn Thr Val 
        675                 680                 685             


Met Asp Glu Leu Trp Gln Thr Ser Arg Asn Phe Met Gln Ile Ile Arg 
    690                 695                 700                 


Gln Ser Asp Phe Gln Thr Ala Ile Ala Ala Val Asn Gln Gln Asp Leu 
705                 710                 715                 720 


Gly Arg Gln Asp Leu Gln Arg Val Ile Asp Asp Leu Tyr Thr Ser Pro 
                725                 730                 735     


Gln Asn Lys Lys Ala Ile Arg Gln Val Leu Ala Val Val Thr Asp Ile 
            740                 745                 750         


Gln Lys Ala Met His Gly Lys Ala Pro Ser Trp Leu Phe Val Glu Val 
        755                 760                 765             


Ala Lys Gly Ala Gln Lys Asn Pro Gln Arg Thr Gln Ser Arg Gln Ser 
    770                 775                 780                 


Lys Leu Ala Glu Ala Tyr Arg Glu Ser Ala Arg Glu Leu Val Asp Glu 
785                 790                 795                 800 


Ala Val Lys Glu Gln Leu Leu Thr Leu Pro Asp Asp Arg Gln Thr Phe 
                805                 810                 815     


Ser Asp Arg Leu Val Leu Tyr Phe Gln Gln Asn Gly Arg Asp Ile Tyr 
            820                 825                 830         


Thr Gly Glu Pro Leu Asp Phe Glu Arg Leu Ser Asn Tyr His Ile Asp 
        835                 840                 845             


His Ile Ile Pro Gln Ala Val Val Lys Asp Asp Ser Leu Asp Asn Arg 
    850                 855                 860                 


Val Leu Thr Ser Asn Gln Ala Asn Leu Glu Lys Gly Ala His Val Pro 
865                 870                 875                 880 


Thr Ile Phe Arg Asp Lys Gln Glu Pro Val Trp Gln Glu Leu Tyr Arg 
                885                 890                 895     


Val Gly Leu Ile Ser His Arg Lys Leu Arg Asn Leu Leu Thr Thr Pro 
            900                 905                 910         


Asp Asp Leu Asp Asp Arg Gly Arg Ala Thr Gly Phe Ile Ala Arg Gln 
        915                 920                 925             


Leu Val Glu Thr Arg Gln Val Ile Lys Leu Val Met Glu Val Leu Ser 
    930                 935                 940                 


Gln Tyr Tyr Asp Thr Thr Glu Thr Glu Leu Ile Ser Ile Lys Ala Gly 
945                 950                 955                 960 


Leu Ser His Gln Phe Arg Gln Glu Leu Asn Leu Pro Lys Leu Arg Ala 
                965                 970                 975     


Leu Asn Asp Tyr His His Ala Gln Asp Ala Tyr Leu Ala Ala Arg Ile 
            980                 985                 990         


Gly Thr Tyr Leu Leu Gln Arg Tyr  Pro Asn Leu Lys Arg  Phe Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Phe Lys Lys Thr  His Phe Asp Leu Gln  Arg Arg Phe 
    1010                 1015                 1020             


Asn Phe  Ile Gly Gly Met Ile  Ser Arg Glu Lys Gln  Pro Tyr Asn 
    1025                 1030                 1035             


Leu Ile  Lys Asn Pro Val Thr  His Gln Val Ile Trp  Asp Glu Glu 
    1040                 1045                 1050             


Met Glu  Ile Gly Asp Cys Gln  Lys Lys Asn Pro Glu  Gln Pro Lys 
    1055                 1060                 1065             


His Asp  Ala Thr Val Lys Met  Met Asn Leu Thr Lys  Ile Met Asn 
    1070                 1075                 1080             


Leu Lys  His Ile Leu Val Thr  His Glu Val Tyr Glu  Asn His Gly 
    1085                 1090                 1095             


Ala Leu  Phe Asn Gln Thr Ile  Tyr Pro Ala Ser Gln  Ala His Lys 
    1100                 1105                 1110             


Lys Lys  Leu Ile Pro Gln Lys  Thr Glu Lys Pro Ile  Glu Leu Tyr 
    1115                 1120                 1125             


Gly Gly  His Ser Ser Glu Gln  Ala Gly Tyr Met Ala  Ile Ile Arg 
    1130                 1135                 1140             


Leu Leu  Gly Lys Arg Pro Gly  Tyr Lys Val Met Arg  Val Pro Val 
    1145                 1150                 1155             


Arg Tyr  Val Ser Gln Leu Gln  His Gln Asp Val Ala  Glu Gln Lys 
    1160                 1165                 1170             


Lys Leu  Leu Tyr Lys Ile Phe  Val Ser Glu Leu Thr  Ser Arg Asn 
    1175                 1180                 1185             


Lys Val  Lys Pro Phe Glu Val  Val Val Pro His Val  Cys Leu Asn 
    1190                 1195                 1200             


Gln Pro  Val Glu Asp Met Ile  Lys Gly Gln Trp His  Arg Phe Ala 
    1205                 1210                 1215             


Leu Arg  Thr Asn Gly Tyr Tyr  Gln Asn Gln Gln Gln  Leu Ile Leu 
    1220                 1225                 1230             


Ser Leu  Val Ala Gln Arg Thr  Leu Ala Gly Tyr Lys  Pro Lys Asp 
    1235                 1240                 1245             


Asp Glu  Leu Met Ala Val Tyr  His Glu Ile Cys Lys  Gln Val Lys 
    1250                 1255                 1260             


Tyr Tyr  Phe Pro Leu Tyr Lys  Met Asn Lys Phe Gln  Ser Arg Leu 
    1265                 1270                 1275             


Phe Ala  Ala Glu Glu Leu Leu  Gln Lys Arg Ser Thr  Val Asn Ser 
    1280                 1285                 1290             


Phe Ser  Ile Thr Ile Gly Lys  Asn Gly Lys Glu Asn  Lys Thr Leu 
    1295                 1300                 1305             


Asn Leu  Gly Lys Arg Glu Leu  Leu Leu Arg Leu Leu  Gly Gly Leu 
    1310                 1315                 1320             


His Ala  Asn Ala Thr Arg Val  Asp Leu Lys Asp Ile  Gly Cys Thr 
    1325                 1330                 1335             


Gly Gln  Phe Gly Gln Leu Gln  Val Lys Asn Gly Ile  Lys Leu Thr 
    1340                 1345                 1350             


Glu Gln  Ala Val Leu Ile His  Glu Ser Pro Thr Gly  Leu Phe His 
    1355                 1360                 1365             


Arg Arg  Val Arg Leu Asn Asp  Leu 
    1370                 1375     


<210>  1073
<211>  1377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1073

Met Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Thr Lys Val Asp Glu 
                85                  90                  95      


Ser Phe Phe Tyr Arg Leu Asp Glu Ser Phe Leu Thr Thr Asp Glu Lys 
            100                 105                 110         


Asp Phe Glu Arg His Pro Ile Phe Gly Asn Lys Ala Asp Glu Ile Lys 
        115                 120                 125             


Tyr His Gln Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn 
                165                 170                 175     


Ala Glu Asn Thr Asp Val Gln Lys Ile Phe Ala Asp Phe Val Gly Val 
            180                 185                 190         


Tyr Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp 
        195                 200                 205             


Ala Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Leu Ile Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly 
225                 230                 235                 240 


Asn Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Met Asn 
                245                 250                 255     


Phe Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr 
            260                 265                 270         


Asn Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Lys Arg Tyr Ala Glu His His Glu Asp Leu Glu Lys Leu 
                325                 330                 335     


Lys Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe 
            340                 345                 350         


Lys Asp Glu Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val 
        355                 360                 365             


Lys Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Asn Thr Leu Ser Lys Ile 
    370                 375                 380                 


Ala Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ser 
    450                 455                 460                 


Trp Ala Glu Tyr His Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Lys Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser His Val Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr Lys Ile 
        515                 520                 525             


Lys Tyr Val Asn Glu Gln Gly Lys Asp Ser Phe Phe Asp Ser Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 


Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg 
                565                 570                 575     


Ile Lys Asp Leu Ile Gly Leu Asp Lys Glu Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys Ala Phe 
        595                 600                 605             


Leu Asp Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu Gln Lys 
625                 630                 635                 640 


Tyr Ser Asp Ile Phe Thr Ala Asp Gln Leu Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asn Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Ser Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu 
    690                 695                 700                 


Pro Phe Lys Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp Val Asp 
705                 710                 715                 720 


Asp Ile Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val 
            740                 745                 750         


Met Gly Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys 
    770                 775                 780                 


Leu Gln Asn Ser Leu Lys Glu Leu Gly Ser Asn Ile Leu Asn Glu Glu 
785                 790                 795                 800 


Lys Pro Ser Tyr Ile Glu Asp Lys Val Glu Asn Ser His Leu Gln Asn 
                805                 810                 815     


Asp Gln Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr Thr 
            820                 825                 830         


Gly Asp Glu Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile Asp His 
        835                 840                 845             


Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val 
    850                 855                 860                 


Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser 
865                 870                 875                 880 


Leu Asp Ile Val Arg Ala Arg Lys Ala Glu Trp Val Arg Leu Tyr Lys 
                885                 890                 895     


Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu 
            900                 905                 910         


Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala Gly Phe Ile Lys Arg Gln 
        915                 920                 925             


Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp 
    930                 935                 940                 


Ala Arg Phe Asn Thr Glu Ser Asp Glu Asn Asp Lys Val Ile Arg Asp 
945                 950                 955                 960 


Val Lys Val Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys 
                965                 970                 975     


Asp Phe Glu Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala 
            980                 985                 990         


His Asp Ala Tyr Leu Asn Ala Val  Val Gly Thr Ala Leu  Leu Lys Lys 
        995                 1000                 1005             


Tyr Pro  Lys Leu Ala Ser Glu  Phe Val Tyr Gly Glu  Tyr Lys Lys 
    1010                 1015                 1020             


Tyr Asp  Val His Lys Leu Ile  Ala Lys Ser Ser Asp  Asp His Ser 
    1025                 1030                 1035             


Glu Met  Gly Lys Ala Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Leu 
    1040                 1045                 1050             


Met Asn  Phe Phe Lys Arg Val  Ile Arg Tyr Ser Asn  Gly Lys Val 
    1055                 1060                 1065             


Ile Val  Arg Pro Val Val Glu  Tyr Ser Lys Asp Thr  Glu Asp Ile 
    1070                 1075                 1080             


Ala Trp  Asp Lys Lys Ser Asn  Phe Arg Thr Ile Cys  Lys Val Leu 
    1085                 1090                 1095             


Ser Tyr  Pro Gln Val Asn Ile  Val Lys Lys Val Glu  Thr Gln Thr 
    1100                 1105                 1110             


Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro Lys Gly  Asp Ser Asp 
    1115                 1120                 1125             


Lys Leu  Ile Pro Arg Lys Thr  Lys Lys Ala Tyr Trp  Asp Thr Lys 
    1130                 1135                 1140             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Val Ala Tyr  Ser Val Phe 
    1145                 1150                 1155             


Val Val  Ala Asp Val Glu Lys  Gly Lys Ala Lys Lys  Leu Lys Thr 
    1160                 1165                 1170             


Val Lys  Glu Leu Val Gly Ile  Ser Ile Met Glu Arg  Ser Phe Phe 
    1175                 1180                 1185             


Glu Glu  Asn Pro Val Glu Phe  Leu Glu Asn Lys Gly  Tyr His Asn 
    1190                 1195                 1200             


Ile Arg  Glu Asp Lys Leu Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe 
    1205                 1210                 1215             


Glu Phe  Glu Gly Gly Lys Arg  Arg Leu Leu Ala Ser  Ala Ser Glu 
    1220                 1225                 1230             


Leu Gln  Lys Gly Asn Glu Met  Val Ile Pro Gly His  Leu Val Lys 
    1235                 1240                 1245             


Leu Leu  Tyr His Ala Gln Arg  Ile Asn Ser Phe Asn  Ser Thr Lys 
    1250                 1255                 1260             


Tyr Leu  Asp Tyr Val Ser Ala  His Lys Lys Glu Phe  Glu Lys Val 
    1265                 1270                 1275             


Leu Ser  Cys Val Glu Asp Phe  Ala Asn Leu Tyr Val  Asp Val Glu 
    1280                 1285                 1290             


Lys Asn  Leu Ser Lys Ile Arg  Ala Val Ala Asp Ser  Met Asp Asn 
    1295                 1300                 1305             


Phe Ser  Ile Glu Glu Ile Ser  Asn Ser Phe Ile Asn  Leu Leu Thr 
    1310                 1315                 1320             


Leu Thr  Ala Leu Gly Ala Pro  Ala Asp Phe Asn Phe  Leu Gly Glu 
    1325                 1330                 1335             


Lys Ile  Pro Arg Lys Arg Tyr  Thr Ser Thr Lys Glu  Cys Leu Asn 
    1340                 1345                 1350             


Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1355                 1360                 1365             


Ile Asp  Leu Ser Lys Ile Gly  Glu Glu 
    1370                 1375         


<210>  1074
<211>  1377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1074

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ser Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Leu Asp Ser Ser 
                85                  90                  95      


Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Glu Tyr 
        115                 120                 125             


His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp Ile 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         


Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             


Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Thr Lys Arg Glu Arg 
    210                 215                 220                 


Val Leu Lys Leu Phe Ser Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     


Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Gly Phe Ala Asp 
        275                 280                 285             


Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Glu Arg Tyr Glu Asn His Gln Glu Asp Leu Ala Ala Leu Lys 
                325                 330                 335     


Gln Phe Ile Lys Asn Asn Leu Ser Glu Lys Tyr Ala Glu Val Phe Ser 
            340                 345                 350         


Asp Gln Ser Lys Asp Gly Tyr Ala Gly Phe Ile Asp Gly Lys Thr Thr 
        355                 360                 365             


Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Leu Glu 
    370                 375                 380                 


Gly Ala Asp Tyr Phe Leu Asn Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Leu Phe 
            420                 425                 430         


Leu Lys Glu Asn Arg Glu Lys Ile Glu Lys Ile Leu Ala Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 


Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     


Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 


Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 


Glu Lys Asp Ile Ile His Tyr Leu His Asn Val Asp Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp Asn 
        595                 600                 605             


Pro Lys Asn Gly Glu Ile Leu Glu Asn Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp Thr 
625                 630                 635                 640 


Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         


Gln Thr Gly Lys Thr Ile Leu Glu Tyr Leu Ile Asp Asp Gly Asp Cys 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe Lys 
    690                 695                 700                 


Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asp Asp Val Lys 
705                 710                 715                 720 


Gln Val Val Gln Glu Ile Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         


Asn Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp 
    770                 775                 780                 


Ala Leu Lys Asn Leu Ala Pro Gly Leu Asp Ser Asn Ile Leu Lys Glu 
785                 790                 795                 800 


Asn Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr 
                805                 810                 815     


Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Lys Ala Ile Asp Ile 
            820                 825                 830         


Asn Gln Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe 
        835                 840                 845             


Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys Asp 
    850                 855                 860                 


Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys 
865                 870                 875                 880 


Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu 
                885                 890                 895     


Arg Lys Phe Asn Asn Leu Thr Lys Ala Lys Arg Gly Gly Leu Asp Glu 
            900                 905                 910         


Arg Asp Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln 
        915                 920                 925             


Ile Thr Lys His Val Ala Gln Leu Leu Asp Thr Arg Phe Asn Thr Glu 
    930                 935                 940                 


Val Asn Glu Glu Asn Gln Lys Ile Arg Thr Val Lys Ile Ile Thr Leu 
945                 950                 955                 960 


Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly Leu Tyr Lys 
                965                 970                 975     


Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn 
            980                 985                 990         


Ala Val Val Ala Lys Ala Ile Leu  Lys Lys Tyr Pro Lys  Leu Glu Pro 
        995                 1000                 1005             


Glu Phe  Val Tyr Gly Asp Tyr  Gln Lys Tyr Asp Leu  Lys Arg Tyr 
    1010                 1015                 1020             


Ile Leu  Lys Ser Lys Ala Ser  Asn Thr Ile Asp Lys  Ala Thr Glu 
    1025                 1030                 1035             


Lys Tyr  Phe Phe Tyr Ser Asn  Leu Leu Asn Phe Phe  Lys Glu Lys 
    1040                 1045                 1050             


Val Arg  Tyr Ala Asp Gly Thr  Ile Lys Lys Arg Glu  Asn Ile Glu 
    1055                 1060                 1065             


Tyr Ser  Asn Asp Thr Gly Glu  Ile Ala Trp Asn Lys  Glu Lys Asp 
    1070                 1075                 1080             


Phe Ala  Thr Ile Lys Lys Val  Leu Ser Leu Pro Gln  Val Asn Ile 
    1085                 1090                 1095             


Val Lys  Lys Thr Glu Glu Gln  Thr Val Gly Gln Asn  Gly Gly Leu 
    1100                 1105                 1110             


Phe Asp  Asn Asn Ile Val Ser  Lys Lys Lys Val Val  Asp Ala Ser 
    1115                 1120                 1125             


Lys Leu  Ile Pro Ile Lys Ser  Ser Leu Ser Pro Glu  Lys Tyr Gly 
    1130                 1135                 1140             


Gly Tyr  Ala Arg Pro Thr Ile  Ala Tyr Ser Val Leu  Val Ile Ala 
    1145                 1150                 1155             


Asp Ile  Glu Lys Gly Lys Gly  Lys Ala Lys Lys Leu  Lys Arg Ile 
    1160                 1165                 1170             


Lys Glu  Ile Val Gly Ile Thr  Ile Gln Asp Lys Lys  Lys Phe Glu 
    1175                 1180                 1185             


Ser Asn  Pro Val Thr Tyr Leu  Glu Glu Cys Gly Tyr  Lys Asn Ile 
    1190                 1195                 1200             


Asn Ser  Asn Leu Ile Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu 
    1205                 1210                 1215             


Phe Asn  Asp Gly Gln Arg Arg  Leu Leu Ala Ser Ser  Ile Glu Leu 
    1220                 1225                 1230             


Gln Lys  Gly Asn Glu Leu Ile  Leu Pro Tyr His Leu  Thr Ala Leu 
    1235                 1240                 1245             


Leu Tyr  His Ala Gln Arg Ile  Asn Lys Ile Ser Glu  Pro Ile His 
    1250                 1255                 1260             


Lys Gln  Tyr Val Glu Ala His  Gln Asn Glu Phe Lys  Glu Leu Leu 
    1265                 1270                 1275             


Thr Thr  Ile Ile Ser Leu Ser  Lys Lys Tyr Ile Gln  Lys Pro Asn 
    1280                 1285                 1290             


Val Glu  Leu Leu Leu Gln Gln  Ala Phe Asp Gln Ala  Asp Lys Asp 
    1295                 1300                 1305             


Ile Tyr  Gln Leu Ser Glu Ser  Phe Ile Ser Leu Leu  Lys Leu Thr 
    1310                 1315                 1320             


Ser Phe  Gly Ala Pro Gly Ala  Phe Lys Phe Leu Gly  Val Glu Ile 
    1325                 1330                 1335             


Ser Gln  Ser Ser Val Arg Tyr  Lys Pro Asn Ser Gln  Phe Leu Asp 
    1340                 1345                 1350             


Thr Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1355                 1360                 1365             


Ile Asp  Leu Ser Lys Leu Gly  Glu Asp 
    1370                 1375         


<210>  1075
<211>  1377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1075

Met Asn Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Arg Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Ala Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Ile Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Val Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Val Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ala Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Thr Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Glu Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Val Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Val Leu 
                325                 330                 335     


Lys Gln Phe Ile Lys Thr Asn Leu Pro Glu Lys Tyr Val Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Asp Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Val Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Thr Ile Leu Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr His Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Met Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Lys Ile Ile Asn Gln Leu Phe Lys Lys Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Asp Asp Ile Ile Asp Cys Leu Gln Lys Ile Asp Asn Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Ile Leu Lys Ile Ile Arg Asp Lys Glu Phe Met Asp 
        595                 600                 605             


Asp Pro Lys Asn Gly Asp Ile Leu Glu Asn Val Ile His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asn 
625                 630                 635                 640 


Ser Ile Phe Asp Glu Lys Val Ile Lys Ala Leu Ser Arg Lys His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asp Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Thr Gly Lys Thr Ile Leu Asp Tyr Leu Met Asp Asp Gly Lys 
        675                 680                 685             


Ser Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Asp Glu Leu Ser Phe 
    690                 695                 700                 


Thr Glu Ile Ile Gln Lys Ala Gln Val Val Gly Asp Thr Asp Asn Leu 
705                 710                 715                 720 


Arg Gln Val Val Gln Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Ala Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ser Leu Lys Asn Leu Ala Ser Gly Leu Asp Ser Asn Ile Leu Lys 
785                 790                 795                 800 


Glu Asn Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asn 
            820                 825                 830         


Ile Asn Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val Val Glu 
865                 870                 875                 880 


Lys Met Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Glu Arg Lys Tyr Asn Asn Leu Thr Lys Ala Glu Arg Glu Arg Gly Gly 
            900                 905                 910         


Leu Asn Glu Leu Asp Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe 
    930                 935                 940                 


Asn Lys Glu Val Thr Glu Lys Asn Lys Lys Asn Arg Asn Val Lys Ile 
945                 950                 955                 960 


Ile Thr Leu Lys Ser Asn Met Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Leu Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Lys Lys  Tyr Pro Lys 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Asp Tyr Gln Lys  Tyr Asp Leu 
    1010                 1015                 1020             


Lys Arg  Tyr Ile Ser Arg Ser  Lys Asp Pro Lys Glu  Val Glu Lys 
    1025                 1030                 1035             


Ala Thr  Glu Lys Tyr Phe Phe  Tyr Ser Asn Leu Leu  Asn Phe Phe 
    1040                 1045                 1050             


Lys Glu  Glu Val His Tyr Ala  Asp Gly Thr Ile Val  Lys Arg Glu 
    1055                 1060                 1065             


Asn Ile  Glu Tyr Ser Lys Asp  Thr Gly Glu Ile Ala  Trp Asn Lys 
    1070                 1075                 1080             


Glu Lys  Asp Phe Ala Thr Ile  Lys Lys Val Leu Ser  Leu Pro Gln 
    1085                 1090                 1095             


Val Asn  Ile Val Lys Lys Thr  Glu Glu Gln Thr Val  Gly Gln Asn 
    1100                 1105                 1110             


Gly Gly  Leu Phe Asp Asn Asn  Ile Val Ser Lys Lys  Lys Val Val 
    1115                 1120                 1125             


Asp Ala  Ser Lys Leu Ile Pro  Ile Lys Ser Gly Leu  Ser Pro Glu 
    1130                 1135                 1140             


Lys Tyr  Gly Gly Tyr Ala Arg  Pro Thr Ile Ala Tyr  Ser Val Leu 
    1145                 1150                 1155             


Val Thr  Tyr Lys Gln Asn Asn  Lys Ile Lys Asn Thr  Met Val Gly 
    1160                 1165                 1170             


Ile Pro  Val Leu Thr Lys Leu  Glu Phe Glu Arg Asp  Pro Asn Leu 
    1175                 1180                 1185             


Tyr Leu  Arg Asn Leu Gly Ile  Asn Asn Val Ser His  Val Ile Lys 
    1190                 1195                 1200             


Leu Val  Lys Tyr Thr Val Leu  Glu Phe Lys His Lys  Tyr Gly Thr 
    1205                 1210                 1215             


Tyr Arg  Arg Phe Ile Val Ser  Asn Gln Glu Leu Lys  Arg Ala Asn 
    1220                 1225                 1230             


Gln Ile  Phe Val Thr Thr Lys  Gln Met Lys Leu Ile  Ser Gln Tyr 
    1235                 1240                 1245             


Thr Thr  Leu Asp Lys Asn Glu  His Asp Ser Leu Leu  Lys Asn Asn 
    1250                 1255                 1260             


Gln Gln  Leu Ile Gln Ser Ile  Trp Asn Gln Phe Leu  Glu Phe Met 
    1265                 1270                 1275             


Leu Ala  Thr Asp Leu Val Asn  Lys Lys Gln Tyr Asp  Glu Leu Ile 
    1280                 1285                 1290             


Leu Thr  Asn Asn Gln Thr Ile  Glu Glu Cys Arg Asn  Ile Met Asn 
    1295                 1300                 1305             


Arg Val  Ile Thr Thr Leu Ser  Phe Thr Asn Ile Asn  Ala Gly Gln 
    1310                 1315                 1320             


Lys Lys  Ile Ile Phe Asp Asp  Thr Leu Ser Leu Pro  Ile Lys Arg 
    1325                 1330                 1335             


Trp Gln  Phe Thr Asp Ser Asp  Lys Met Val Lys Lys  Ile Ile Glu 
    1340                 1345                 1350             


Ser Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1355                 1360                 1365             


Ile Asp  Leu Ser Lys Leu Gly  Glu Asp 
    1370                 1375         


<210>  1076
<211>  1377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1076

Met Glu Ser Asn His Asp Gly Glu Gln Val Lys Tyr Thr Leu Gly Leu 
1               5                   10                  15      


Asp Ile Gly Thr Ser Ser Ile Gly Tyr Ala Ala Ile Asp Lys His Gln 
            20                  25                  30          


Lys Pro Ile Arg Ala Lys Gly Lys His Val Ile Gly Val Arg Leu Phe 
        35                  40                  45              


Gln Glu Gly Gln Thr Ala Ala Asp Arg Arg Ala Phe Arg Thr Thr Arg 
    50                  55                  60                  


Arg Arg Leu Lys Arg Arg Lys Trp Arg Leu Asn Leu Leu Asn Arg Leu 
65                  70                  75                  80  


Phe Asp Pro Tyr Leu Ser Glu Val Asp Pro Asn Phe Leu Pro Arg Leu 
                85                  90                  95      


Arg Gln Ser Asn Leu Ser Pro Lys Asp Asp Asn Lys His Phe Asn Gly 
            100                 105                 110         


Ser Thr Leu Phe Pro Asp Lys Thr Asp Ala Ala Phe Tyr Arg Gln Tyr 
        115                 120                 125             


Pro Thr Met Tyr His Leu Arg Tyr Ala Leu Met Thr Glu Lys Arg Lys 
    130                 135                 140                 


Phe Asp Ile Arg Glu Val Tyr Leu Ala Ile His His Ile Val Lys Tyr 
145                 150                 155                 160 


Arg Gly Asn Phe Leu Asn Ser Ala Thr Val Asp Ser Phe Lys Thr Ser 
                165                 170                 175     


Asn Ile Asp Phe Thr Ser Gln Phe Asp Arg Leu Asn Glu Leu Tyr Arg 
            180                 185                 190         


Gln Val Ile Leu Asp Glu Pro Phe Gln Ile Asn Met Asp Gln Val Asp 
        195                 200                 205             


Glu Met Thr Asn Lys Leu Leu Asp Asn Asp Ala Leu Lys Leu Asp Thr 
    210                 215                 220                 


Gln Lys Gln Val Ala Lys Leu Leu Pro Val Ile Tyr Asn Asp Lys Pro 
225                 230                 235                 240 


Ile Asp Lys Gln Tyr Thr Arg Ile Ala Thr Glu Phe Ser Lys Ala Ile 
                245                 250                 255     


Leu Gly Tyr Lys Thr Lys Leu Asp Val Ile Leu Asn Leu Asp Thr Gln 
            260                 265                 270         


Asn Ala Lys Asp Trp Val Ile Arg Leu Asp Asp Glu Asp Ile Asp Asp 
        275                 280                 285             


Lys Leu Pro Ala Leu Val Glu Asn Leu Asp Glu Ser Arg Gln Glu Ile 
    290                 295                 300                 


Val Thr Ile Ile Arg Asp Leu Tyr Ala Gln Ile Thr Leu Asn Ala Ile 
305                 310                 315                 320 


Val Pro Lys Gly Lys Ser Leu Ser Glu Ser Met Val Asp Lys Tyr Asn 
                325                 330                 335     


Asp His Lys Asp His Leu Asp Leu Leu Met Gly Leu Ile His Glu Leu 
            340                 345                 350         


Gly Asp Asp Ser Ala Lys Gly Ile Lys Leu Arg Glu Ala Tyr Thr Gln 
        355                 360                 365             


Tyr Val Gly Lys Ser Asp Asp Lys Thr Leu Asn Gln Asp Asp Phe Tyr 
    370                 375                 380                 


Ala Ala Ile Lys Lys Asn Leu Asp Glu Asp Ser Lys Arg Ser Pro Lys 
385                 390                 395                 400 


Ile Gln Arg Leu Ile Glu Gln Ala Ser Phe Met Pro Lys Gln Arg Thr 
                405                 410                 415     


Ser Ala Asn Gly Val Ile Pro His Gln Leu His Gln Leu Glu Leu Asp 
            420                 425                 430         


Arg Ile Ile Glu Asn Gln Gly Arg Tyr Tyr Pro Phe Leu Lys Lys Pro 
        435                 440                 445             


Asn Pro Asn Thr His Lys Pro Asn Gly Gly Lys Tyr Lys Leu Asp Glu 
    450                 455                 460                 


Leu Val Ala Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ile Thr Lys 
465                 470                 475                 480 


Arg Asp Gln Glu Lys Thr Ser Gly Ala Asp Phe Ala Trp Met Ile Arg 
                485                 490                 495     


Lys Pro Gly Gln Glu Gly Glu Ile Thr Pro Trp Asn Phe Asp Gln Lys 
            500                 505                 510         


Val Asp Arg Met Ala Ser Ala Asn Arg Phe Ile Arg Arg Met Thr Thr 
        515                 520                 525             


Lys Asp Ser Tyr Leu Val Ala Glu Asp Val Leu Pro Asp Ala Ser Leu 
    530                 535                 540                 


Ile Tyr Glu Lys Phe Lys Ala Leu Asn Glu Leu Asn Met Leu Lys Val 
545                 550                 555                 560 


Asn Asp Ser Arg Leu Ser Pro Ser Gln Lys Gln Asp Leu Phe Asn Asn 
                565                 570                 575     


Leu Phe Lys Lys Gln Lys Thr Ile Arg Thr Lys Lys Leu Gln Thr Tyr 
            580                 585                 590         


Ile Arg Thr Asn Trp Glu Leu Pro Ser Val Met Ile Ser Gly Leu Ala 
        595                 600                 605             


Asp Pro Asp Lys Phe Asn Ser Ser Leu Ser Thr Tyr Ile Asp Phe Arg 
    610                 615                 620                 


Asp Ile Phe Gly Glu Lys Val Asp Asp Pro Asn Arg Gln Ala Asp Tyr 
625                 630                 635                 640 


Glu Glu Ile Ile Glu Trp Ser Thr Val Phe Glu Asp Arg Lys Ile Tyr 
                645                 650                 655     


Gln Ala Lys Leu Asn Gln Leu Asp Trp Leu Thr Glu Pro Gln Arg Lys 
            660                 665                 670         


Ala Leu Leu Thr Lys Arg Tyr Thr Gly Trp Gly Arg Leu Ser Lys Lys 
        675                 680                 685             


Leu Leu Thr Gly Leu Lys Asp Gln Asn Gly Asp Ser Ile Leu Asp Gln 
    690                 695                 700                 


Leu Trp Lys Thr Asn Asp Asn Phe Met Gln Ile Gln Ser Arg Asp Glu 
705                 710                 715                 720 


Phe Ala Lys Gln Ile His Asp Glu Asn Ala Lys Gln Phe His Glu Ala 
                725                 730                 735     


Asn Asn Val Asp Asp Ile Leu Asp Asp Ala Phe Thr Ser Pro Gln Asn 
            740                 745                 750         


Lys Lys Ala Ile Arg Gln Val Asp Lys Val Val Gln Asp Val Val Lys 
        755                 760                 765             


Ala Val Gly Tyr Ala Pro Asp Lys Ile Ala Ile Glu Phe Thr Arg Ser 
    770                 775                 780                 


Leu Glu Asn Asn Pro Gln Arg Thr Val Ser Arg Gln Arg Gln Leu Gln 
785                 790                 795                 800 


Ala Ala Tyr Lys Asn Ile Pro Arg Glu Ile Met Lys Ser Gly Leu Met 
                805                 810                 815     


Ala Glu Leu Gly Ser Val Ala Asp Ser Lys Lys Val Leu Ser Asp Lys 
            820                 825                 830         


Leu Tyr Leu Tyr Phe Thr Gln Met Gly Lys Asp Met Tyr Glu Asp Lys 
        835                 840                 845             


Thr Ile Asn Ile Asp Asn Leu Pro Asn Tyr Asp Ile Asp His Ile Leu 
    850                 855                 860                 


Pro Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Ile 
865                 870                 875                 880 


Ser Lys Glu Glu Asn Ala Arg Lys Ser Asn Arg Val Pro Leu Lys Phe 
                885                 890                 895     


Phe Gly Ala Lys Arg Met Gly Phe Trp Lys Asp Leu Leu Asn Asn Gly 
            900                 905                 910         


Leu Ile Ser Asn Arg Lys Phe Gln Asn Leu Thr Thr Asp Pro Glu Lys 
        915                 920                 925             


Ile Ser Lys Tyr Ser Ala Lys Gly Phe Ile His Arg Gln Leu Val Glu 
    930                 935                 940                 


Thr Ser Gln Val Ile Arg Leu Val Ala Asn Ile Leu Gly Asn Glu Tyr 
945                 950                 955                 960 


Ser Gln Ala Gly Thr Thr Ile Ile Glu Val Ser Ala Lys Met Asn Arg 
                965                 970                 975     


Gln Leu Arg Glu Asp Phe His Leu Ile Lys Asn Arg Asn Val Asn Asp 
            980                 985                 990         


Tyr His His Ala Met Asp Ala Tyr  Leu Thr Ala Tyr Val  Gly Asp Tyr 
        995                 1000                 1005             


Leu Tyr  Leu Arg Tyr Pro Lys  Leu Arg Pro Tyr Phe  Val Tyr Gly 
    1010                 1015                 1020             


Asp Phe  Lys Lys Ala Glu Asp  Gln Asp Leu Asn Ile  Arg Asn Phe 
    1025                 1030                 1035             


Asn Phe  Leu His Asp Leu Lys  Asp Asn Glu Ser Leu  Asp Lys Ile 
    1040                 1045                 1050             


Val Asp  Ser Glu Thr Gly Glu  Ile Val Trp Glu Lys  His Gly Ser 
    1055                 1060                 1065             


Val Lys  Gln Leu Lys Lys Val  Tyr His Tyr Lys Phe  Met Leu Val 
    1070                 1075                 1080             


Ser Gln  Glu Val Tyr Thr Arg  Gln Asp Ala Leu Phe  Asn Gln Thr 
    1085                 1090                 1095             


Ile Tyr  Pro Ala Ser Asp Ala  Asp Lys Arg Lys Leu  Ile Pro Ile 
    1100                 1105                 1110             


Lys Asn  Ser Lys Pro Val Asp  Val Tyr Gly Gly Tyr  Ser Gly Asn 
    1115                 1120                 1125             


Ile Asp  Ala Tyr Met Ala Ile  Val Arg Val His Gly  Lys Lys Glu 
    1130                 1135                 1140             


Asp Asn  Tyr Lys Val Val Gly  Ile Pro Met Arg Ala  Val Thr Lys 
    1145                 1150                 1155             


Leu Lys  Lys Ala Glu Lys Glu  Gly His Asp Ser Tyr  Leu Asn Ala 
    1160                 1165                 1170             


Val His  Glu Val Leu Lys Pro  Gln Phe Thr Lys Lys  Lys Lys Asn 
    1175                 1180                 1185             


Arg Lys  Thr Gly Glu Ile Thr  Glu Thr Val Asp Glu  Phe Asp Val 
    1190                 1195                 1200             


Leu Leu  Gly Lys Val Tyr Tyr  Arg Gln Leu Ile Val  Asp Gly Asn 
    1205                 1210                 1215             


Lys Lys  Phe Met Leu Gly Ser  Ser Ser Tyr Gln Tyr  Asn Ala Lys 
    1220                 1225                 1230             


Gln Leu  Val Leu Ser Asp Lys  Ala Met Gln Val Leu  Ser Lys Asn 
    1235                 1240                 1245             


Gly Lys  Val Lys Ser Gln Asp  Glu Asn Gln Asn Leu  Ile Asp Val 
    1250                 1255                 1260             


Tyr Asp  Glu Ile Leu Glu Lys  Val Asp Gln Tyr Phe  Glu Leu Tyr 
    1265                 1270                 1275             


Asp Gln  Arg His Tyr Arg Glu  Ser Leu His Lys Gly  Arg Gly Ile 
    1280                 1285                 1290             


Phe Val  Asn Leu Pro Leu Ser  Asn Gln Tyr Asp Gly  Ser Lys Leu 
    1295                 1300                 1305             


Thr Ser  Tyr Gly Lys Tyr Glu  Thr Ile Asp Ala Ile  Leu Asn Gly 
    1310                 1315                 1320             


Leu His  Ala Asn Ala Thr Met  Ser Asn Leu Arg Tyr  Leu Gly Ile 
    1325                 1330                 1335             


Ser Ser  Pro Phe Gly Lys Met  Gln Ala Ser Asn Gly  Leu Thr Ile 
    1340                 1345                 1350             


Asp Pro  Asp Ser Ile Ile Cys  Tyr Gln Ser Pro Thr  Gly Leu Phe 
    1355                 1360                 1365             


Glu Arg  Gln Ile Lys Leu Ser  Asp Leu 
    1370                 1375         


<210>  1077
<211>  1377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1077

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Tyr Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Arg Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Lys Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn His Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Gln Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Val Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His His Glu Asp Leu Lys His Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Asn Tyr Arg Glu Val Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Leu Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Phe Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Arg Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Phe Leu Ala Glu Gly Phe Lys Asp Phe Gln Phe Leu Asn Arg Lys 
    530                 535                 540                 


Gln Lys Glu Thr Ile Phe Asn Ser Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Ser Phe Leu Asn Lys Val Asp Gly Tyr Glu 
                565                 570                 575     


Gly Ile Ala Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Gly Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Thr Asp Asn Glu Leu Ile Leu Glu Asp Ile Val Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Lys Cys Leu Asp Ile Tyr Lys Asp 
625                 630                 635                 640 


Phe Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asp Leu Ser Phe Lys 
    690                 695                 700                 


Pro Ile Ile Asp Lys Ala Arg Thr Gly Ser His Ser Asp Asn Leu Lys 
705                 710                 715                 720 


Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Leu Ser Arg Ser Arg Gln Arg Leu Thr Thr Leu Arg Glu 
    770                 775                 780                 


Ser Leu Ala Asn Leu Lys Ser Asn Ile Leu Glu Glu Lys Lys Pro Lys 
785                 790                 795                 800 


Tyr Val Lys Asp Gln Val Glu Asn His His Leu Ser Asp Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Lys Lys Ala 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Ile Glu Ile 
865                 870                 875                 880 


Val Lys Ala Arg Lys Met Phe Trp Lys Asn Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Val Asp Asn Gly Lys Lys Ile Cys Lys Val Lys Ile Val 
945                 950                 955                 960 


Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly Phe 
                965                 970                 975     


Tyr Lys Ile Arg Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr 
            980                 985                 990         


Leu Asn Ala Val Val Ala Lys Ala  Ile Leu Thr Lys Tyr  Pro Gln Leu 
        995                 1000                 1005             


Glu Pro  Glu Phe Val Tyr Gly  Met Tyr Arg Gln Lys  Lys Leu Ser 
    1010                 1015                 1020             


Lys Ile  Val His Glu Asp Lys  Glu Glu Lys Tyr Ser  Glu Ala Thr 
    1025                 1030                 1035             


Arg Lys  Met Phe Phe Tyr Ser  Asn Leu Met Asn Met  Phe Lys Arg 
    1040                 1045                 1050             


Val Val  Arg Leu Ala Asp Gly  Ser Ile Val Val Arg  Pro Val Ile 
    1055                 1060                 1065             


Glu Thr  Gly Arg Tyr Met Arg  Lys Thr Ala Trp Asp  Lys Lys Lys 
    1070                 1075                 1080             


His Phe  Ala Thr Val Arg Lys  Val Leu Ser Tyr Pro  Gln Asn Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Thr Glu Ile  Gln Thr Gly Gly Phe  Ser Lys Glu 
    1100                 1105                 1110             


Ser Ile  Leu Ala His Gly Asn  Ser Asp Lys Leu Ile  Pro Arg Lys 
    1115                 1120                 1125             


Thr Lys  Asp Ile Tyr Leu Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp 
    1130                 1135                 1140             


Ser Pro  Ile Val Ala Tyr Ser  Val Leu Val Val Ala  Asp Ile Lys 
    1145                 1150                 1155             


Lys Gly  Lys Ala Gln Lys Leu  Lys Thr Val Thr Glu  Leu Leu Gly 
    1160                 1165                 1170             


Ile Thr  Ile Met Glu Arg Ser  Arg Phe Glu Lys Asn  Pro Ser Ala 
    1175                 1180                 1185             


Phe Leu  Glu Ser Lys Gly Tyr  Leu Asn Ile Arg Asp  Asp Lys Leu 
    1190                 1195                 1200             


Met Ile  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1205                 1210                 1215             


Arg Arg  Leu Leu Ala Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu 
    1220                 1225                 1230             


Leu Ala  Leu Pro Thr Gln Phe  Met Lys Phe Leu Tyr  Leu Ala Ser 
    1235                 1240                 1245             


Arg Tyr  Asn Glu Ser Lys Gly  Lys Pro Glu Glu Ile  Glu Lys Lys 
    1250                 1255                 1260             


Gln Glu  Phe Val Asn Gln His  Val Ser Tyr Phe Asp  Asp Ile Leu 
    1265                 1270                 1275             


Gln Leu  Ile Asn Asp Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala 
    1280                 1285                 1290             


Asn Leu  Glu Lys Ile Asn Lys  Leu Tyr Gln Asp Asn  Lys Glu Asn 
    1295                 1300                 1305             


Ile Pro  Val Asp Glu Leu Ala  Asn Asn Ile Ile Asn  Leu Phe Thr 
    1310                 1315                 1320             


Phe Thr  Ser Leu Gly Ala Pro  Ala Ala Phe Lys Phe  Phe Asp Lys 
    1325                 1330                 1335             


Ile Val  Asp Arg Lys Arg Tyr  Thr Ser Thr Lys Glu  Val Leu Asn 
    1340                 1345                 1350             


Ser Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1355                 1360                 1365             


Ile Asp  Leu Gly Lys Leu Gly  Glu Asp 
    1370                 1375         


<210>  1078
<211>  1377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1078

Met Glu Lys Thr Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Ala Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Lys Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         


Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Thr Tyr 
        115                 120                 125             


His Gln Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ser Pro Glu Lys Val Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Gln Leu Asn Ala 
                165                 170                 175     


Glu Asn Thr Asp Val Gln Lys Ile Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         


Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             


Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Lys Gln Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Val Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     


Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             


Leu Phe Thr Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Glu Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     


Thr Phe Ile Lys Val Asn Asn Phe Asp Lys Tyr His Glu Ile Phe Lys 
            340                 345                 350         


Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             


Gln Asp Ile Phe Tyr Lys His Leu Lys Ser Ile Ile Ser Glu Lys Asn 
    370                 375                 380                 


Gly Gly Gln Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Thr Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         


Leu Lys Glu Asn Gln Ala Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Ala Lys Tyr His Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 


Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     


Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Tyr Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             


Tyr Val Asn Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 


Ala Lys Leu Leu Ser Tyr Leu Asn Asn Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     


Asn Asp Leu Ile Gly Leu Asp Lys Asp Ser Lys Ser Phe Asn Ala Ser 
            580                 585                 590         


Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys Ser Phe Leu 
        595                 600                 605             


Asp Asp Lys Thr Asn Glu Gln Ile Ile Glu Asp Ile Val Leu Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr 
625                 630                 635                 640 


Ser Asp Ile Phe Thr Ser Gln Gln Leu Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Asn Asn Lys Thr Ile Leu Asp Phe Leu Ile Asp Asp Gly 
        675                 680                 685             


Asp Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser 
    690                 695                 700                 


Phe Lys Thr Thr Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp 
705                 710                 715                 720 


Ile Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly His Asn Pro Gln Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Ile Thr Gly Tyr Gly Arg Asn Arg Ser Asn Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 


Gln Asp Ser Leu Lys Glu Phe Gly Ser Asp Ile Leu Ser Lys Lys Lys 
785                 790                 795                 800 


Pro Ser Tyr Val Asp Ser Lys Val Glu Asn Ser His Leu Gln Asn Asp 
                805                 810                 815     


Arg Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr Thr Gly 
            820                 825                 830         


Glu Glu Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile Asp His Ile 
        835                 840                 845             


Ile Pro Gln Ala Phe Ile Lys Asp Asn Ser Ile Asp Asn Arg Val Leu 
    850                 855                 860                 


Thr Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Ile 
865                 870                 875                 880 


Glu Ile Val Arg Asn Arg Lys Ser Tyr Trp Tyr Lys Leu Tyr Lys Ser 
                885                 890                 895     


Gly Leu Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
            900                 905                 910         


Gly Gly Leu Thr Glu Thr Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala 
    930                 935                 940                 


Arg Phe Asn Thr Lys Cys Asp Glu Asn Asp Lys Val Ile Arg Asp Val 
945                 950                 955                 960 


Lys Val Ile Thr Leu Lys Ser Ser Leu Val Ser Gln Phe Arg Lys Glu 
                965                 970                 975     


Phe Lys Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Gly Thr Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Lys  Leu Ala Pro Glu Phe  Val Tyr Gly Glu Tyr  Lys Lys Tyr 
    1010                 1015                 1020             


Asp Val  Arg Lys Leu Val Ala  Lys Ser Ser Asp Asn  His Ser Glu 
    1025                 1030                 1035             


Leu Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe Tyr Ser  Asn Leu Met 
    1040                 1045                 1050             


Asn Phe  Phe Lys Thr Glu Val  Lys Tyr Ala Asp Gly  Arg Val Phe 
    1055                 1060                 1065             


Glu Arg  Pro Asp Ile Glu Thr  Asn Ala Asp Gly Glu  Val Val Trp 
    1070                 1075                 1080             


Asn Lys  Gln Arg Asp Phe Asn  Ile Val Arg Lys Val  Leu Ser Tyr 
    1085                 1090                 1095             


Pro Gln  Val Asn Ile Val Lys  Lys Val Glu Val Gln  Thr Gly Gly 
    1100                 1105                 1110             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly Asp Ser  Asp Lys Leu 
    1115                 1120                 1125             


Ile Pro  Arg Lys Thr Lys Lys  Leu Gln Trp Glu Thr  Gln Lys Tyr 
    1130                 1135                 1140             


Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr Ser Val  Leu Val Val 
    1145                 1150                 1155             


Ala Asp  Val Glu Lys Gly Lys  Thr Arg Lys Leu Lys  Thr Val Lys 
    1160                 1165                 1170             


Glu Leu  Val Gly Ile Ser Ile  Met Glu Arg Ser Ser  Phe Glu Glu 
    1175                 1180                 1185             


Asn Pro  Val Ser Phe Leu Glu  Lys Lys Gly Tyr His  Asn Val Gln 
    1190                 1195                 1200             


Glu Asp  Lys Leu Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Phe 
    1205                 1210                 1215             


Glu Gly  Gly Arg Arg Arg Leu  Leu Ala Ser Ala Thr  Glu Leu Gln 
    1220                 1225                 1230             


Lys Gly  Asn Glu Val Val Leu  Pro Gln Tyr Met Val  Asn Leu Leu 
    1235                 1240                 1245             


Tyr His  Ser Gln His Val Asn  Asn Ser His Lys Pro  Glu His Leu 
    1250                 1255                 1260             


Asn Tyr  Val Lys Gln His Lys  Asp Glu Phe Lys Asp  Ile Phe Asn 
    1265                 1270                 1275             


Leu Ile  Ile Ser Ile Ala Arg  Ile Asn Ile Leu Lys  Pro Lys Val 
    1280                 1285                 1290             


Val Asp  Asn Leu Ile Asn Glu  Phe Thr Glu Tyr Gly  Gln Glu Asp 
    1295                 1300                 1305             


Ile Ser  Ser Leu Ser Glu Ser  Phe Ile Asn Leu Leu  Lys Phe Ile 
    1310                 1315                 1320             


Ser Phe  Gly Ala Pro Gly Ala  Phe Lys Phe Leu Lys  Leu Asp Val 
    1325                 1330                 1335             


Lys Gln  Ser Asn Leu Arg Tyr  Lys Ser Thr Thr Glu  Ala Leu Ser 
    1340                 1345                 1350             


Ala Thr  Leu Ile His Gln Ser  Val Thr Gly Leu Tyr  Glu Thr Arg 
    1355                 1360                 1365             


Ile Asp  Leu Ser Lys Leu Gly  Glu Glu 
    1370                 1375         


<210>  1079
<211>  1378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1079

Met Lys Thr Phe Ala Glu Glu Tyr Val Met Asp Lys Ser Lys Pro Tyr 
1               5                   10                  15      


Gly Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly Phe Val Ala Thr 
            20                  25                  30          


Asp Ala Glu Gly His Leu Ile Arg Leu Lys Gly Lys Thr Val Ile Gly 
        35                  40                  45              


Ala Tyr Leu Phe Asn Ala Gly Ile Ser Ala Ala Glu Arg Arg Gly Phe 
    50                  55                  60                  


Arg Thr Thr Arg Arg Arg Leu Ser Arg Val Lys Trp Arg Leu Gly Leu 
65                  70                  75                  80  


Leu Arg Glu Ile Phe Glu Thr His Phe Gln Glu Ser Met Gly Glu Asn 
                85                  90                  95      


Glu Asp Asn Asp Phe Phe Leu Arg Phe Lys Tyr Ser Asn Ile Ser Pro 
            100                 105                 110         


Lys Asp Pro Gln Phe Ser Thr Ala Lys Gly Leu Phe Asn Asp Arg Thr 
        115                 120                 125             


Asp Lys Glu Phe Tyr Asp Gln Tyr Pro Thr Ile Tyr His Leu Arg Arg 
    130                 135                 140                 


Ala Leu Met Thr Glu Asp His Gln Phe Asp Ile Arg Glu Ile Tyr Ile 
145                 150                 155                 160 


Ala Met His His Ile Val Lys Tyr Arg Gly His Phe Leu Lys Glu Gly 
                165                 170                 175     


Arg Ala Lys Asp Phe Lys Val Gly Asp Leu Arg Leu Leu Asp Asn Phe 
            180                 185                 190         


Lys Met Met Asn Glu Gln Ile Glu Glu Ile Asn Pro Leu Trp Gln Leu 
        195                 200                 205             


Lys Leu Pro Thr Asp Asp Ala Ser Ile Lys Ser Ile Thr Ala Ile Leu 
    210                 215                 220                 


Leu Asp Asn Thr Gln Ser Gln Asn Asp Arg Gln Lys Ala Val Thr Lys 
225                 230                 235                 240 


Val Ile Leu Ala Thr Leu Val Lys Ala Ser Asp Lys Asp Ile Asn Ala 
                245                 250                 255     


Ala Arg Lys Arg Phe Val Gly Glu Leu Ser Lys Ala Met Val Gly Leu 
            260                 265                 270         


Lys Thr Lys Leu Trp Val Leu Ala Asp Val Ser Gln Asn Gly Asp Trp 
        275                 280                 285             


Glu Ile Lys Tyr Glu Asn Tyr Ala Asp Phe Ala Glu Thr Ile Gly Ser 
    290                 295                 300                 


Gly Glu Ser Asp Thr Ile Gln Ser Leu Phe Asn Glu Ile Asn Asp Leu 
305                 310                 315                 320 


Tyr Gly Val Ile Thr Leu Ala Gly Ile Ile Pro Lys Glu Ala Glu Ser 
                325                 330                 335     


Phe Ser Asp Gly Met Val Arg Lys Tyr Glu His His Arg Lys Asn Leu 
            340                 345                 350         


Glu Leu Leu Lys Val Tyr Cys Ala Glu Gln Ser Asp Gly Lys Arg Gly 
        355                 360                 365             


Arg Gln Ile Arg Gln Thr Tyr Asp Lys Tyr Ile Asp Gly Val Asp Ser 
    370                 375                 380                 


Lys Gln Phe Thr Gln Glu Asp Phe Tyr Lys Ala Leu Ser Lys Phe Thr 
385                 390                 395                 400 


Ala Lys Asp Glu Ala Thr Ser Glu Asn Ala Lys Leu Ile Ala Gln Glu 
                405                 410                 415     


Ile Ala Val Gly Thr Phe Met Pro Lys Leu Arg Thr Lys Ala Asn Gly 
            420                 425                 430         


Thr Ile Pro His Gln Leu His Gln Lys Glu Leu Asp Ala Ile Ile Glu 
        435                 440                 445             


Asn Gln Lys Lys Tyr Tyr Pro Trp Leu Gly Glu Val Asn Pro Val Glu 
    450                 455                 460                 


Ser His Arg Arg Ala Leu Pro Tyr Lys Leu Asp Glu Leu Val Ser Phe 
465                 470                 475                 480 


Arg Ile Pro Tyr Tyr Val Gly Pro Met Val Thr Pro Thr Lys Gly Asp 
                485                 490                 495     


Pro Glu Lys Ser Lys Phe Ala Trp Met Val Arg Lys Glu Pro Gly Thr 
            500                 505                 510         


Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Ser Ala Ser Gly 
        515                 520                 525             


Glu Ala Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Phe Leu Ile Gly 
    530                 535                 540                 


Glu Asp Val Leu Pro Gln Gln Ser Leu Leu Tyr Gln Lys Phe Glu Val 
545                 550                 555                 560 


Leu Asn Glu Leu Asn Lys Ile Met Ile Asn Gly Lys Pro Ile Cys Arg 
                565                 570                 575     


Glu Gln Lys Gln Arg Leu Phe Lys Gln Leu Phe Met Gln Tyr Lys Thr 
            580                 585                 590         


Val Thr Val Lys Lys Val Gln Gln Asn Leu Ile Ala Asn Gly Glu Glu 
        595                 600                 605             


Ser Glu Asn Val Pro Ile Thr Gly Leu Ser Asp Pro Leu Arg Phe Asn 
    610                 615                 620                 


Ser Ser Phe Ser Thr Tyr Ile Asp Tyr Lys Asp Ile Leu Gly Thr Ala 
625                 630                 635                 640 


Ala Val Asn Asp Asn Ala Lys Gln Ser Asp Ile Glu Gln Ile Ile Ala 
                645                 650                 655     


Trp Ser Thr Ile Phe Glu Asp Ala Ala Ile Phe Arg Glu Lys Leu Asn 
            660                 665                 670         


Asp Ile Thr Trp Leu Asn Asp Asp Gln Arg Asn Lys Leu Ser His Lys 
        675                 680                 685             


Arg Tyr Arg Gly Trp Gly Arg His Ser Arg Lys Leu Leu Ala Gly Leu 
    690                 695                 700                 


Arg Asp Gly Glu Gly Gln Thr Ile Ile Glu Arg Leu Trp Asn Thr Asn 
705                 710                 715                 720 


Asp Asn Phe Met Gln Ile Gln Asn Asp Ser Glu Ile Ala Arg Gln Ile 
                725                 730                 735     


Thr Glu Ala Asn Ser Ser Lys Met Ala Thr Ala Glu Gly Thr Asp Glu 
            740                 745                 750         


Ile Ile Asp Gly Phe Tyr Thr Ser Pro Glu Asn Lys Lys Ala Leu Arg 
        755                 760                 765             


Glu Val Met Lys Val Val Lys Asp Ile Gln Arg Ala His His Gly Gln 
    770                 775                 780                 


Ala Pro Ala Trp Val Tyr Ile Glu Ser Pro Arg Glu Thr Pro Arg Pro 
785                 790                 795                 800 


Gly Gln Arg Thr Val Ser Arg Glu Gln Gln Leu Thr Asp Leu Tyr Glu 
                805                 810                 815     


Gly Ala Ala Lys Glu Ile Val Asp Asp Ala Val Leu Asn Glu Leu Lys 
            820                 825                 830         


Asp Lys Val Lys Ser Lys Glu Asn Phe Thr Asp Lys Leu Val Leu Tyr 
        835                 840                 845             


Phe Leu Gln Asn Gly His Asp Ile Tyr Ala Asn Asp Ser Ile Asn Ile 
    850                 855                 860                 


Asp Asn Leu Asn Ala Tyr Asp Ile Asp His Val Leu Pro Gln Ser Leu 
865                 870                 875                 880 


Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Thr His Glu Arg 
                885                 890                 895     


Asn Leu Lys Lys Ser Asn Arg Phe Ala Thr Glu Leu Phe Ala Asp Gln 
            900                 905                 910         


Arg Lys Lys Trp Glu Lys Trp His Arg Leu Gly Leu Ile Ser Ser Arg 
        915                 920                 925             


Lys Leu Lys His Leu Thr Met Gln Pro Asn Ser Val Glu Lys Phe Ala 
    930                 935                 940                 


His Gly Phe Ile Ala Arg Gln Leu Thr Glu Thr Arg Gln Ile Ile His 
945                 950                 955                 960 


Leu Thr Ala Asn Val Leu Ser Asn Leu Tyr Gln Glu Asn Asp Thr Lys 
                965                 970                 975     


Ile Val Met Ile Lys Ala Gly Leu Asn Ser Glu Phe Arg Arg Thr Phe 
            980                 985                 990         


Asp Phe Pro Lys Asn Arg Ser Val  Asn Asp Tyr His His  Ala Phe Asp 
        995                 1000                 1005             


Ala Phe  Leu Thr Ala Lys Ile  Gly Arg Tyr Leu Leu  Ala Arg Tyr 
    1010                 1015                 1020             


Pro Lys  Leu Glu Pro Phe Phe  Val Tyr Gly Asn Phe  Val Lys Asn 
    1025                 1030                 1035             


Pro Lys  Ala Met Lys Arg Leu  Ser Ser Phe Asp Phe  Ile Ala Gln 
    1040                 1045                 1050             


Leu Ala  Ala Lys Thr Asp Asp  Thr Ser His Ile Asp  Gln Arg Ser 
    1055                 1060                 1065             


Leu Lys  Gln Val Pro Val Val  Asn Glu Glu Thr Gly  Glu Ile Val 
    1070                 1075                 1080             


Trp Asp  Lys Asp Ile Glu Leu  Ala Glu Leu Asp Lys  Thr Tyr Asn 
    1085                 1090                 1095             


Tyr Lys  Thr Met Leu Val Lys  Arg Ala Gln Thr Glu  Asn Asn Ala 
    1100                 1105                 1110             


Gln Met  Phe Lys Gln Thr Val  Phe Lys Ala Arg Asp  Asn Gln Asn 
    1115                 1120                 1125             


Lys Thr  Leu Ile Pro Val Lys  Asn Gly Leu Ser Thr  Asp Val Tyr 
    1130                 1135                 1140             


Gly Gly  His Ser Gln Gln Ala  Ile Ser Tyr Leu Cys  Ile Val Trp 
    1145                 1150                 1155             


Val Gly  Gln Lys Lys Lys Tyr  Arg Val Leu Gly Ile  Ser Thr Ala 
    1160                 1165                 1170             


His Ala  Gly Ile Leu Asn Asn  Phe Glu Lys Asn Tyr  Gly Arg Phe 
    1175                 1180                 1185             


Glu Ala  Lys Lys Lys Leu Gln  Glu Ile Val Ser Asn  Thr Leu Asp 
    1190                 1195                 1200             


Asn Ala  Asp Arg Asn Asp Phe  Lys Ile Val Ala Pro  Lys Val Leu 
    1205                 1210                 1215             


Phe Glu  Gln Val Val Glu Asp  Asp Asn Met Lys Phe  Gly Leu Gly 
    1220                 1225                 1230             


Ser Ala  Ser Asp Tyr Arg Asn  Val Gln Gln Leu Phe  Leu Ser Arg 
    1235                 1240                 1245             


Lys Asn  Gln Leu Leu Leu Ala  Asn Met Met Thr Asp  Gln Ile His 
    1250                 1255                 1260             


Asp Gln  Asp Leu Val His Leu  Phe Asp Glu Ile Val  Gly Gln Met 
    1265                 1270                 1275             


Asn Ala  His Phe Pro Ile Phe  Asp Arg Gly Gly Tyr  Arg Ser Ser 
    1280                 1285                 1290             


Leu Thr  Gln Ser Arg Asp Lys  Phe Leu Lys Leu Pro  Phe Lys Lys 
    1295                 1300                 1305             


Asn Glu  Asp Leu Ile Thr Lys  Gln Glu Val Ile Arg  Arg Ile Leu 
    1310                 1315                 1320             


Asp Gly  Leu His Ala Asn Ala  Asn Arg Lys Asp Leu  Lys Ile Ile 
    1325                 1330                 1335             


Gly Ser  Lys Gly Asp Phe Gly  Arg Leu Gly Thr Lys  Lys Ile Tyr 
    1340                 1345                 1350             


Leu Ser  Lys Asp Ala Lys Leu  Ile Tyr Thr Ser Pro  Thr Cys Leu 
    1355                 1360                 1365             


Phe Thr  Arg Thr Val Pro Leu  Ser Ser Leu 
    1370                 1375             


<210>  1080
<211>  1378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1080

Met Thr Asn Glu Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Phe Ala Val Val Asn Asp Asn Asn Arg Val Ile Arg Val Lys 
            20                  25                  30          


Gly Lys Asn Ala Ile Gly Val Arg Leu Phe Asp Glu Gly Lys Ala Ala 
        35                  40                  45              


Ala Asp Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Arg Trp Arg Leu Lys Leu Leu Arg Glu Ile Phe Asp Ala Tyr Ile Thr 
65                  70                  75                  80  


Pro Val Asp Glu Ala Phe Phe Ile Arg Leu Lys Glu Ser Asn Leu Ser 
                85                  90                  95      


Pro Lys Asp Ser Lys Lys Gln Tyr Ser Gly Asp Ile Leu Phe Asn Asp 
            100                 105                 110         


Arg Ser Asp Lys Asp Phe Tyr Glu Lys Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Asn Ala Leu Met Thr Glu His Arg Lys Phe Asp Val Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Met Lys Phe Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Ala Thr Pro Ala Asn Asn Phe Lys Val Gly Arg Leu Asn Leu Glu Glu 
                165                 170                 175     


Lys Phe Glu Glu Leu Asn Asp Ile Tyr Gln Arg Val Phe Pro Asp Glu 
            180                 185                 190         


Ser Ile Glu Phe Arg Thr Asp Asn Leu Glu Gln Ile Lys Glu Val Leu 
        195                 200                 205             


Leu Asp Asn Lys Arg Ser Arg Ala Asp Arg Gln Arg Thr Leu Val Ser 
    210                 215                 220                 


Asp Ile Tyr Gln Ser Ser Glu Asp Lys Asp Ile Glu Lys Arg Asn Lys 
225                 230                 235                 240 


Ala Val Ala Thr Glu Ile Leu Lys Ala Ser Leu Gly Asn Lys Ala Lys 
                245                 250                 255     


Leu Asn Val Ile Thr Asn Val Glu Val Asp Lys Glu Ala Ala Lys Glu 
            260                 265                 270         


Trp Ser Ile Thr Phe Asp Ser Glu Ser Ile Asp Asp Asp Leu Ala Lys 
        275                 280                 285             


Ile Glu Gly Gln Met Thr Asp Asp Gly His Glu Ile Ile Glu Val Leu 
    290                 295                 300                 


Arg Ser Leu Tyr Ser Gly Ile Thr Leu Ser Ala Ile Val Pro Glu Asn 
305                 310                 315                 320 


His Thr Leu Ser Gln Ser Met Val Ala Lys Tyr Asp Leu His Lys Asp 
                325                 330                 335     


His Leu Lys Leu Phe Lys Lys Leu Ile Asn Gly Met Thr Asp Thr Lys 
            340                 345                 350         


Lys Ala Lys Asn Leu Arg Ala Ala Tyr Asp Gly Tyr Ile Asp Gly Val 
        355                 360                 365             


Lys Gly Lys Val Leu Pro Gln Glu Asp Phe Tyr Lys Gln Val Gln Val 
    370                 375                 380                 


Asn Leu Asp Asp Ser Ala Glu Ala Asn Glu Ile Gln Thr Tyr Ile Asp 
385                 390                 395                 400 


Gln Asp Ile Phe Met Pro Lys Gln Arg Thr Lys Ala Asn Gly Ser Ile 
                405                 410                 415     


Pro His Gln Leu Gln Gln Gln Glu Leu Asp Gln Ile Ile Glu Asn Gln 
            420                 425                 430         


Lys Ala Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Pro Asp Lys 
        435                 440                 445             


Lys Arg Gln Gln Leu Ala Lys Tyr Lys Leu Asp Glu Leu Val Thr Phe 
    450                 455                 460                 


Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Ala Lys Asp Gln Lys 
465                 470                 475                 480 


Asn Gln Ser Gly Ala Glu Phe Ala Trp Met Ile Arg Lys Glu Pro Gly 
                485                 490                 495     


Asn Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Met Ala Thr 
            500                 505                 510         


Ala Asn Gln Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu 
        515                 520                 525             


Gly Glu Asp Val Leu Pro Ala Gln Ser Leu Leu Tyr Gln Lys Phe Glu 
    530                 535                 540                 


Val Leu Asn Glu Leu Asn Lys Ile Arg Ile Asp His Lys Pro Ile Ser 
545                 550                 555                 560 


Ile Glu Gln Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Phe Lys 
                565                 570                 575     


Asn Val Thr Ile Lys His Leu Gln Asp Tyr Leu Val Ser Gln Gly Gln 
            580                 585                 590         


Tyr Ser Lys Arg Pro Leu Ile Glu Gly Leu Ala Asp Glu Lys Arg Phe 
        595                 600                 605             


Asn Ser Ser Leu Ser Thr Tyr Ser Asp Leu Cys Gly Ile Phe Gly Ala 
    610                 615                 620                 


Lys Leu Val Glu Glu Asn Asp Arg Gln Glu Asp Leu Glu Lys Ile Ile 
625                 630                 635                 640 


Glu Trp Ser Thr Ile Phe Glu Asp Lys Lys Ile Tyr Arg Ala Lys Leu 
                645                 650                 655     


Asn Asp Leu Thr Trp Leu Thr Asp Asp Gln Lys Glu Lys Leu Ala Thr 
            660                 665                 670         


Lys Arg Tyr Gln Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Val Gly 
        675                 680                 685             


Leu Lys Asn Ser Glu His Arg Asn Ile Met Asp Ile Leu Trp Ile Thr 
    690                 695                 700                 


Asn Glu Asn Phe Met Gln Ile Gln Ala Glu Pro Asp Phe Ala Lys Leu 
705                 710                 715                 720 


Val Thr Asp Ala Asn Lys Gly Met Leu Glu Lys Thr Asp Ser Gln Asp 
                725                 730                 735     


Val Ile Asn Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Ile Leu Leu Val Val His Asp Ile Gln Asn Ala Met His Gly Gln 
        755                 760                 765             


Ala Pro Ala Lys Ile His Val Glu Phe Ala Arg Gly Glu Glu Arg Asn 
    770                 775                 780                 


Pro Arg Arg Ser Val Gln Arg Gln Arg Gln Val Glu Ala Ala Tyr Glu 
785                 790                 795                 800 


Lys Val Ser Asn Glu Leu Val Ser Ala Lys Val Arg Gln Glu Phe Lys 
                805                 810                 815     


Glu Ala Ile Asn Asn Lys Arg Asp Phe Lys Asp Arg Leu Leu Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Ile Asp Ile Tyr Thr Gly Lys Gln Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser Ser Tyr Gln Ile Asp His Ile Leu Pro Gln Ala Phe 
    850                 855                 860                 


Val Lys Asp Asp Ser Leu Thr Asn Arg Val Leu Thr Asn Glu Asn Gln 
865                 870                 875                 880 


Val Lys Ala Asp Ser Val Pro Ile Asp Ile Phe Gly Lys Lys Met Leu 
                885                 890                 895     


Ser Val Trp Gly Arg Met Lys Asp Gln Gly Leu Ile Ser Lys Gly Lys 
            900                 905                 910         


Tyr Arg Asn Leu Thr Met Asn Pro Glu Asn Ile Ser Ala His Thr Glu 
        915                 920                 925             


Asn Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val Ile Lys 
    930                 935                 940                 


Leu Ala Val Asn Ile Leu Ala Asp Glu Tyr Gly Asp Ser Thr Gln Ile 
945                 950                 955                 960 


Ile Ser Val Lys Ala Asp Leu Ser His Gln Met Arg Glu Asp Phe Glu 
                965                 970                 975     


Leu Leu Lys Asn Arg Asp Val Asn Asp Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Tyr Leu Ala Ala Phe Ile Gly Asn  Tyr Leu Leu Lys Arg  Tyr Pro Lys 
        995                 1000                 1005             


Leu Glu  Ser Tyr Phe Val Tyr  Gly Asp Phe Lys Lys  Phe Thr Gln 
    1010                 1015                 1020             


Lys Glu  Thr Lys Met Arg Arg  Phe Asn Phe Ile Tyr  Asp Leu Lys 
    1025                 1030                 1035             


His Cys  Asp Gln Val Val Asn  Lys Glu Thr Gly Glu  Ile Leu Trp 
    1040                 1045                 1050             


Thr Lys  Asp Glu Asp Ile Lys  Tyr Ile Arg His Leu  Phe Ala Tyr 
    1055                 1060                 1065             


Lys Lys  Ile Leu Val Ser His  Glu Val Arg Glu Lys  Arg Gly Ala 
    1070                 1075                 1080             


Leu Tyr  Asn Gln Thr Ile Tyr  Lys Ala Lys Asp Asp  Lys Gly Ser 
    1085                 1090                 1095             


Gly Gln  Gly Ser Lys Lys Leu  Ile Arg Ile Lys Asp  Asp Lys Glu 
    1100                 1105                 1110             


Thr Lys  Ile Tyr Gly Gly Tyr  Ser Gly Lys Ser Leu  Ala Tyr Met 
    1115                 1120                 1125             


Thr Ile  Val Gln Ile Thr Lys  Lys Asn Lys Val Ser  Tyr Arg Val 
    1130                 1135                 1140             


Ile Gly  Ile Pro Thr Leu Ala  Leu Ala Arg Leu Asn  Lys Leu Glu 
    1145                 1150                 1155             


Asn Asp  Ser Thr Glu Asn Asn  Gly Glu Leu Tyr Lys  Ile Ile Lys 
    1160                 1165                 1170             


Pro Gln  Phe Thr His Tyr Lys  Val Asp Lys Lys Asn  Gly Glu Ile 
    1175                 1180                 1185             


Ile Glu  Thr Thr Asp Asp Phe  Lys Ile Val Val Ser  Lys Val Arg 
    1190                 1195                 1200             


Phe Gln  Gln Leu Ile Asp Asp  Ala Gly Gln Phe Phe  Met Leu Ala 
    1205                 1210                 1215             


Ser Asp  Thr Tyr Lys Asn Asn  Ala Gln Gln Leu Val  Ile Ser Asn 
    1220                 1225                 1230             


Asn Ala  Leu Lys Ala Ile Asn  Asn Thr Asn Ile Thr  Asp Cys Pro 
    1235                 1240                 1245             


Arg Asp  Asp Leu Glu Arg Leu  Asp Asn Leu Arg Leu  Asp Ser Ala 
    1250                 1255                 1260             


Phe Asp  Glu Ile Val Lys Lys  Met Asp Lys Tyr Phe  Ser Ala Tyr 
    1265                 1270                 1275             


Asp Ala  Asn Asn Phe Arg Glu  Lys Ile Arg Asn Ser  Asn Leu Ile 
    1280                 1285                 1290             


Phe Tyr  Gln Leu Pro Val Glu  Asp Gln Trp Glu Asn  Asn Lys Ile 
    1295                 1300                 1305             


Thr Glu  Leu Gly Lys Arg Thr  Val Leu Thr Arg Ile  Leu Gln Gly 
    1310                 1315                 1320             


Leu His  Ala Asn Ala Thr Thr  Thr Asp Met Ser Ile  Phe Lys Ile 
    1325                 1330                 1335             


Lys Thr  Pro Phe Gly Gln Leu  Arg Gln Arg Ser Gly  Ile Ser Leu 
    1340                 1345                 1350             


Ser Glu  Asn Ala Gln Leu Ile  Tyr Gln Ser Pro Thr  Gly Leu Phe 
    1355                 1360                 1365             


Glu Arg  Arg Val Gln Leu Asn  Lys Ile Lys 
    1370                 1375             


<210>  1081
<211>  1378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1081

Met Lys Asn Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Phe Ala Val Val Asp Glu Asn Asn His Val Met Arg Val Lys 
            20                  25                  30          


Gly Lys Asn Ala Ile Gly Val Arg Leu Phe Asp Glu Gly Lys Pro Ala 
        35                  40                  45              


Ala Asp Arg Arg Ala Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Arg Trp Arg Leu Lys Leu Leu Arg Glu Ile Phe Asp Ser Tyr Ile Thr 
65                  70                  75                  80  


Pro Val Asp Glu Ala Phe Phe Ile Arg Leu Lys Glu Ser Asn Leu Ser 
                85                  90                  95      


Pro Lys Asp Ser Lys Lys Gln Tyr Ser Gly Asp Ile Leu Phe Asn Asp 
            100                 105                 110         


Arg Ser Asp Lys Asp Phe Tyr Thr Lys Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Asn Ala Leu Met Thr Glu His Gln Arg Phe Asp Ile Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Val Lys Phe Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Ala Thr Pro Ala Asn Asn Phe Gln Val Gly Lys Leu Asp Leu Asp Gln 
                165                 170                 175     


Lys Phe Glu Asp Leu Asn Asp Ile Tyr Gln Arg Val Phe Pro Asp Asp 
            180                 185                 190         


Ser Ile Glu Phe Arg Ile Asp Asn Leu Glu Gln Ile Lys Ala Val Leu 
        195                 200                 205             


Leu Asp Ser Lys Arg Ser Arg Ser Asp Arg Gln Arg Ser Leu Val Asp 
    210                 215                 220                 


Asp Val Tyr Gln Thr Ser Glu Asp Lys Glu Val Glu Lys Gln Asn Lys 
225                 230                 235                 240 


Ala Val Ala Thr Glu Val Leu Lys Ala Ile Leu Gly Asn Lys Ala Lys 
                245                 250                 255     


Leu Asn Ile Ile Ala Asn Val Glu Val Asp Lys Glu Ala Ala Lys Glu 
            260                 265                 270         


Trp Thr Ile Thr Phe Asp Ser Glu Ser Ile Asp Asp Asp Leu Ala Lys 
        275                 280                 285             


Ile Asp Gly Gln Met Thr Asp Asp Gly Arg Ala Ile Val Glu Ile Leu 
    290                 295                 300                 


Arg Ser Leu Tyr Ser Gly Ile Thr Leu Ser Ala Ile Val Pro Glu Asn 
305                 310                 315                 320 


His Thr Leu Ser Gln Ala Met Val Ala Lys Tyr Asp Leu His Lys Lys 
                325                 330                 335     


His Leu Thr Leu Leu Lys Lys Leu Ile Asn Ser Met Thr Asp Thr Lys 
            340                 345                 350         


Lys Ala Lys Asn Leu Arg Ala Ala Tyr Asp Gly Tyr Val Asp Gly Val 
        355                 360                 365             


Lys Gly Lys Val Leu Thr Gln Asp Glu Phe Tyr Lys Gln Ile Gln Ser 
    370                 375                 380                 


Asn Leu Asp Glu Ser Val Glu Ala Ser Glu Ile Gln Thr Tyr Ile Asp 
385                 390                 395                 400 


Gln Asp Ile Phe Met Pro Lys Gln Arg Thr Lys Ala Asn Gly Ser Ile 
                405                 410                 415     


Pro His Gln Leu Gln Gln Gln Glu Leu Asp Gln Ile Ile Glu Asn Gln 
            420                 425                 430         


Lys Glu Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Pro Asp Lys 
        435                 440                 445             


Lys Arg Gln Gln Leu Ala Lys Tyr Lys Leu Asp Glu Leu Val Thr Phe 
    450                 455                 460                 


Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Ala Glu Ala Gln Lys 
465                 470                 475                 480 


Lys Gln Ser Gly Ala Glu Phe Ala Trp Met Ile Arg Lys Glu Ser Gly 
                485                 490                 495     


Asn Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Met Ala Thr 
            500                 505                 510         


Ala Asn Gln Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu 
        515                 520                 525             


Gly Glu Asp Val Leu Pro Ser Gln Ser Leu Leu Tyr Gln Arg Phe Glu 
    530                 535                 540                 


Val Leu Asn Glu Leu Asn Lys Ile Arg Ile Asp Arg Lys Pro Ile Ser 
545                 550                 555                 560 


Val Glu Leu Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Lys Phe Lys 
                565                 570                 575     


Asn Val Thr Ile Lys His Leu Gln Asp Tyr Leu Val Ala Gln Gly Gln 
            580                 585                 590         


Tyr Ser Lys Arg Pro Leu Ile Glu Gly Leu Ala Asp Glu Lys Arg Phe 
        595                 600                 605             


Asn Ser Gly Leu Ser Thr Tyr Asn Asp Phe Cys Gly Ile Phe Gly Ala 
    610                 615                 620                 


Lys Leu Val Asp Glu Asn Asp Arg Gln Ala Asp Leu Glu Lys Ile Ile 
625                 630                 635                 640 


Glu Trp Ser Thr Ile Phe Glu Asp Lys Lys Ile Tyr Arg Ala Lys Leu 
                645                 650                 655     


Asn Asp Leu Ala Trp Leu Thr Asp Glu Gln Lys Asp Lys Leu Ala Thr 
            660                 665                 670         


Lys Arg Tyr Gln Gly Trp Gly Arg Leu Ser Ser Lys Leu Leu Ile Gly 
        675                 680                 685             


Leu Gln Asn Ser Glu His Arg Asn Ile Met Asp Leu Leu Trp Ile Gly 
    690                 695                 700                 


Asn Glu Asn Phe Met Gln Ile Gln Ala Asp Ser Asp Phe Ala Lys Leu 
705                 710                 715                 720 


Ile Lys Asp Ala Asn Gln Asn Ala Leu Gln Asn Asn Asp Ser Gln Asp 
                725                 730                 735     


Val Ile Asn Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Ile Leu Leu Val Val His Asp Ile Gln Asn Ala Met His Gly Gln 
        755                 760                 765             


Ala Pro Thr Lys Ile His Val Glu Phe Ala Lys Gly Glu Glu Arg Asn 
    770                 775                 780                 


Pro Arg Arg Ser Val Gln Arg Gln Arg Gln Val Glu Ala Ala Tyr Glu 
785                 790                 795                 800 


Lys Ala Ser Asn Glu Leu Val Gly Ala Asn Val Arg Glu Glu Leu Lys 
                805                 810                 815     


Asp Ala Ile Thr Asn Lys Arg Asp Phe Lys Asp Arg Leu Phe Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Val Asp Met Tyr Ser Gly Glu Pro Ile Asn Ile 
        835                 840                 845             


Asp Asn Leu Ser Arg Tyr Asp Ile Asp His Ile Leu Pro Gln Ala Phe 
    850                 855                 860                 


Val Lys Asp Asp Ser Leu Asn Asn Arg Val Leu Thr Asp Ala Glu Lys 
865                 870                 875                 880 


Asn Arg Arg Lys Ser Asp Ala Val Pro Asn Glu Leu Phe Gly Ala Lys 
                885                 890                 895     


Met Arg Val Thr Trp Glu Arg Met Lys Glu Gln Gly Leu Ile Ser Lys 
            900                 905                 910         


Gly Lys Tyr Arg Asn Leu Thr Met Asn Pro Glu Asn Ile Asn Lys Tyr 
        915                 920                 925             


Thr Glu Asn Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val 
    930                 935                 940                 


Ile Lys Leu Ala Val Asn Ile Leu Ala Asp Glu Tyr Lys Glu Asn Thr 
945                 950                 955                 960 


Gln Ile Ile Ser Val Lys Ala Asp Leu Ser His Gln Met Arg Glu Asp 
                965                 970                 975     


Phe Glu Leu Leu Lys Asn Arg Asp Val Asn Asp Tyr His His Ala Phe 
            980                 985                 990         


Asp Ala Tyr Leu Ala Ala Phe Met  Gly Asn Tyr Leu Leu  Lys Arg Tyr 
        995                 1000                 1005             


Pro Lys  Leu Arg Ser Tyr Phe  Val Tyr Gly Asp Phe  Lys Lys Phe 
    1010                 1015                 1020             


Thr Gln  Lys Glu Ile Lys Leu  Arg Arg Phe Asn Phe  Ile Tyr Asp 
    1025                 1030                 1035             


Leu Lys  His Glu Asp Gln Val  Ile Asp Lys Glu Thr  Gly Glu Ile 
    1040                 1045                 1050             


Leu Trp  Asn Lys Asn Glu Asp  Ile Ala Tyr Ile Arg  His Leu Phe 
    1055                 1060                 1065             


Ala Tyr  Lys Lys Ile Leu Ile  Ser His Glu Val Ser  Glu Arg Arg 
    1070                 1075                 1080             


Gly Ala  Leu Tyr Asn Gln Thr  Val Tyr Lys Ala Ser  Asp Asp Lys 
    1085                 1090                 1095             


Ala Ser  Gly Gln Gly Ser Lys  Lys Leu Ile Pro Ile  Lys Ser Asp 
    1100                 1105                 1110             


Lys Pro  Thr Glu Ile Tyr Gly  Gly Tyr Thr Gly Arg  Thr Leu Ala 
    1115                 1120                 1125             


Tyr Met  Thr Ile Val Lys Val  Thr Lys Gly Lys Lys  Ile Ser Tyr 
    1130                 1135                 1140             


Lys Val  Val Gly Val Pro Thr  Ala Val Leu Ala Lys  Met Ala Gly 
    1145                 1150                 1155             


Val Arg  Gln Gly Ser Gln Glu  Glu Gln Asp Lys Leu  Tyr Gly Val 
    1160                 1165                 1170             


Leu Lys  Pro Gln Phe Thr His  Gln Lys Glu Asn Lys  Lys Thr Gly 
    1175                 1180                 1185             


Glu Ile  Thr Glu Thr Val Asp  Lys Phe Asp Ile Ile  Val Pro Lys 
    1190                 1195                 1200             


Val Lys  Tyr Gln Gln Leu Val  Asn Asp Asn Gly Gln  Leu Phe Met 
    1205                 1210                 1215             


Ile Gly  Ser Asp Thr Leu Pro  Tyr Asn Ala Glu Gln  Leu Val Leu 
    1220                 1225                 1230             


Ser Lys  Glu Ser Val Gln Thr  Ile Asn Asn Pro Gly  Ile Lys Asp 
    1235                 1240                 1245             


Glu Gly  Ser Asp Glu Glu Ser  Glu Asn Lys Lys Leu  Asp Asn Val 
    1250                 1255                 1260             


Tyr Asp  Glu Ile Val Leu Cys  Met Asn Lys Tyr Phe  Pro Leu Tyr 
    1265                 1270                 1275             


Asp Asn  Arg Gly Phe Arg Asn  Ser Ile Thr Asn Gly  Lys Asn Lys 
    1280                 1285                 1290             


Phe Leu  Lys Leu Pro Ile Lys  Asn Ile Tyr Lys Asn  Gly Lys Leu 
    1295                 1300                 1305             


Ile Asn  Val Gly Lys Arg Glu  Val Ile Asn Arg Ile  Leu Gln Gly 
    1310                 1315                 1320             


Leu His  Ala Asn Ala Ser Val  Thr Asp Leu Ser Asn  Leu Gly Ile 
    1325                 1330                 1335             


Lys Ser  Pro Phe Gly Phe Leu  Gln Lys Gly Ala Gly  Leu Ser Leu 
    1340                 1345                 1350             


Ser Gly  Glu Ala Gln Leu Ile  His Gln Ser Ala Thr  Gly Leu Phe 
    1355                 1360                 1365             


Glu His  Arg Val Gln Leu Asn  Lys Ile Lys 
    1370                 1375             


<210>  1082
<211>  1378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1082

Met Ser Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Gly Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Asn Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Met Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Glu Gln Asn Val Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Glu Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Gly Phe Ala 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ser Leu 
                325                 330                 335     


Lys Gln Phe Ile Lys Asn Asn Leu Pro Ala Glu Lys Cys Val Glu Val 
            340                 345                 350         


Phe Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys 
        355                 360                 365             


Thr Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys 
    370                 375                 380                 


Leu Glu Gly Ala Tyr Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe 
385                 390                 395                 400 


Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile 
                405                 410                 415     


His Leu Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr 
            420                 425                 430         


Pro Phe Leu Lys Glu Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe 
        435                 440                 445             


Arg Ile Pro Tyr His Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe 
    450                 455                 460                 


Ala Trp Leu Thr Arg Asn Ser Asp Glu Ala Ile Arg Pro Trp Asn Phe 
465                 470                 475                 480 


Glu Glu Ile Val Asp Gln Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys 
                485                 490                 495     


Met Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys 
            500                 505                 510         


His Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys 
        515                 520                 525             


Val Lys Phe Ile Ala Glu Gly Leu Thr Arg Tyr Gln Phe Leu Asp Lys 
    530                 535                 540                 


Lys Gln Lys Lys Asp Ile Phe Tyr Thr Phe Phe Lys Ala Glu Asp Lys 
545                 550                 555                 560 


Arg Lys Val Thr Glu Lys Asp Ile Ile His Tyr Leu His Asn Val Asp 
                565                 570                 575     


Gly Tyr Asp Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala 
            580                 585                 590         


Ser Leu Ser Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu 
        595                 600                 605             


Phe Met Asp Asp Pro Lys Asn Glu Glu Ile Leu Glu Asn Ile Val His 
    610                 615                 620                 


Thr Leu Thr Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala 
625                 630                 635                 640 


Gln Tyr Asp Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg 
                645                 650                 655     


Arg His Tyr Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly 
            660                 665                 670         


Ile Cys Asp Lys Gln Thr Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp 
        675                 680                 685             


Asp Gly Lys Ile Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly 
    690                 695                 700                 


Leu Ser Phe Lys Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr 
705                 710                 715                 720 


Tyr Asp Val Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile 
                725                 730                 735     


Lys Lys Gly Ile Leu Gln Ser Val Lys Leu Val Asp Glu Leu Val Lys 
            740                 745                 750         


Val Met Gly His Ala Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu 
        755                 760                 765             


Asn Gln Thr Thr Ala Lys Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys 
    770                 775                 780                 


Arg Ile Glu Asp Ala Ile Lys Asn Leu Ala Pro Gly Leu Asp Ser Thr 
785                 790                 795                 800 


Ile Leu Lys Glu His Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg 
                805                 810                 815     


Leu Phe Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Lys 
            820                 825                 830         


Pro Leu Glu Ile Asn Gln Leu Ser Asn Tyr Asp Ile Asp His Ile Ile 
        835                 840                 845             


Pro Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr 
    850                 855                 860                 


Ser Ser Lys Glu Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu 
865                 870                 875                 880 


Val Val Glu Lys Met Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys 
                885                 890                 895     


Leu Ile Ser Glu Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly 
            900                 905                 910         


Gly Leu Asp Glu Leu Asp Lys Val Gly Phe Ile Lys Arg Gln Leu Val 
        915                 920                 925             


Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg 
    930                 935                 940                 


Phe Asn Lys Glu Val Thr Glu Lys Asn Lys Lys Asn Arg Asn Val Lys 
945                 950                 955                 960 


Ile Ile Thr Leu Lys Ser Asn Met Val Ser Asn Phe Arg Lys Glu Phe 
                965                 970                 975     


Gly Leu Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp 
            980                 985                 990         


Ala Tyr Leu Asn Ala Val Val Ala  Lys Ala Ile Leu Lys  Lys Tyr Pro 
        995                 1000                 1005             


Lys Leu  Glu Pro Glu Phe Val  Tyr Gly Asp Tyr Gln  Lys Tyr Asp 
    1010                 1015                 1020             


Leu Lys  Arg Tyr Ile Ser Lys  Ser Lys Asp Pro Lys  Glu Val Glu 
    1025                 1030                 1035             


Lys Ala  Thr Glu Lys Tyr Phe  Phe Tyr Ser Asn Leu  Leu Asn Phe 
    1040                 1045                 1050             


Phe Lys  Glu Glu Val His Tyr  Ala Asp Gly Thr Ile  Val Lys Arg 
    1055                 1060                 1065             


Glu Asn  Ile Glu Tyr Ser Lys  Asp Thr Gly Glu Ile  Ala Trp Asn 
    1070                 1075                 1080             


Lys Glu  Lys Asp Phe Ala Thr  Ile Lys Lys Val Leu  Ser Phe Pro 
    1085                 1090                 1095             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Glu Gln Thr  Val Gly Gln 
    1100                 1105                 1110             


Asn Gly  Gly Leu Phe Asp Asn  Asn Ile Val Ser Lys  Glu Lys Val 
    1115                 1120                 1125             


Val Asp  Ala Ser Lys Leu Ile  Pro Ile Lys Ser Gly  Leu Ser Pro 
    1130                 1135                 1140             


Glu Lys  Tyr Gly Gly Tyr Ala  Arg Pro Thr Ile Ala  Tyr Ser Val 
    1145                 1150                 1155             


Leu Val  Thr Tyr Lys Gln Asn  Asn Lys Ile Lys Asn  Thr Met Val 
    1160                 1165                 1170             


Gly Ile  Pro Val Leu Thr Lys  Leu Glu Phe Glu Arg  Asp Pro Asn 
    1175                 1180                 1185             


Leu Tyr  Leu Arg Asn Leu Gly  Ile Asn Asn Val Ser  His Val Ile 
    1190                 1195                 1200             


Lys Leu  Val Lys His Thr Val  Leu Glu Phe Lys His  Lys Tyr Gly 
    1205                 1210                 1215             


Thr Tyr  Arg Arg Phe Ile Val  Ser Asn Gln Glu Leu  Lys Arg Ala 
    1220                 1225                 1230             


Asn Gln  Ile Phe Val Thr Thr  Lys Gln Met Lys Leu  Ile Ser Gln 
    1235                 1240                 1245             


Tyr Thr  Thr Leu Asp Lys Asn  Glu His Asp Ser Leu  Leu Lys Asn 
    1250                 1255                 1260             


Asn Gln  Gln Leu Ile Gln Ser  Ile Trp Asn Gln Phe  Leu Glu Phe 
    1265                 1270                 1275             


Met Leu  Ala Thr Asp Leu Val  Asn Lys Lys Gln Tyr  Asp Glu Leu 
    1280                 1285                 1290             


Ile Leu  Thr Asn Asn Gln Thr  Ile Glu Glu Cys Arg  Asn Ile Met 
    1295                 1300                 1305             


Asn Arg  Val Ile Thr Thr Leu  Ser Phe Thr Asn Ile  Asn Ala Gly 
    1310                 1315                 1320             


Gln Lys  Lys Ile Ile Phe Asp  Asp Thr Leu Ser Leu  Pro Ile Lys 
    1325                 1330                 1335             


Arg Trp  Gln Phe Thr Asp Ser  Asp Lys Met Val Lys  Lys Ile Ile 
    1340                 1345                 1350             


Glu Ser  Thr Leu Ile His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr 
    1355                 1360                 1365             


Arg Ile  Asp Leu Asn Lys Leu  Gly Glu Asp 
    1370                 1375             


<210>  1083
<211>  1378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1083

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Met Gln Glu Glu Lys Tyr Tyr 
        115                 120                 125             


His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Arg Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Gln Lys Gln Tyr Gln Ala Phe Leu Glu Ile 
            180                 185                 190         


Phe Asp Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Arg Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Ile Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His His Glu Asp Leu Lys His Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Asn Tyr Arg Glu Val Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Ser Gln Glu Ala Phe Tyr Lys Tyr Leu Leu Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Gly Ser Glu Tyr Leu Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Arg Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Leu Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Phe Leu Ala Glu Gly Phe Lys Asp Phe Gln Phe Leu Asn Arg Lys 
    530                 535                 540                 


Gln Lys Glu Thr Ile Phe Asn Ser Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Ser Phe Leu Asn Lys Val Asp Gly Tyr Glu 
                565                 570                 575     


Gly Ile Ala Ile Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Lys Lys Ile Leu Gly Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Thr Asp Asn Glu Leu Ile Leu Glu Asp Ile Ile Gln Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Asp Ile Tyr Lys Asp 
625                 630                 635                 640 


Phe Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         


Glu Asn Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile Lys Asp Ala Gly Leu Ser Phe Lys 
    690                 695                 700                 


Pro Ile Ile Asp Lys Ala Arg Thr Gly Ser His Ser Asp Asn Leu Lys 
705                 710                 715                 720 


Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         


Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             


Ala Lys Gly Leu Ser Arg Ser Arg Gln Arg Leu Thr Thr Leu Arg Glu 
    770                 775                 780                 


Ser Leu Gly Asn Leu Lys Ser Asn Ile Leu Glu Glu Lys Lys Pro Lys 
785                 790                 795                 800 


Tyr Val Lys Asp Gln Val Glu Asn His His Leu Ser Asp Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Asp Glu 
            820                 825                 830         


Leu Asp Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Ile Glu Ile 
865                 870                 875                 880 


Val Lys Ala Arg Lys Met Phe Trp Lys Asn Leu Leu Asp Ala Lys Leu 
                885                 890                 895     


Met Ser Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe 
    930                 935                 940                 


Asn Asn Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile 
945                 950                 955                 960 


Val Thr Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly 
                965                 970                 975     


Phe Tyr Lys Ile Arg Glu Val Asn Asn Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Ala Lys  Ala Ile Leu Thr Lys  Tyr Pro Gln 
        995                 1000                 1005             


Leu Glu  Pro Glu Phe Val Tyr  Gly Leu Tyr Arg Arg  Lys Lys Leu 
    1010                 1015                 1020             


Ser Lys  Ile Val Arg Glu Asp  Lys Glu Glu Lys Tyr  Ser Glu Ala 
    1025                 1030                 1035             


Thr Arg  Lys Met Phe Phe Tyr  Ser Asn Leu Met Asn  Met Phe Lys 
    1040                 1045                 1050             


Arg Val  Val Arg Leu Ala Asp  Gly Ser Ile Val Val  Arg Pro Val 
    1055                 1060                 1065             


Ile Glu  Thr Gly Arg Tyr Met  Gly Lys Thr Ala Trp  Asp Lys Lys 
    1070                 1075                 1080             


Lys His  Phe Ala Thr Val Arg  Lys Val Leu Ser Tyr  Pro Gln Asn 
    1085                 1090                 1095             


Asn Ile  Val Lys Lys Thr Glu  Ile Gln Thr Gly Gly  Phe Ser Lys 
    1100                 1105                 1110             


Glu Ser  Ile Leu Ala His Gly  Asn Ser Asp Lys Leu  Ile Pro Arg 
    1115                 1120                 1125             


Lys Thr  Lys Asp Ile Tyr Leu  Asp Pro Lys Lys Tyr  Gly Gly Phe 
    1130                 1135                 1140             


Asp Ser  Pro Ile Val Ala Tyr  Ser Val Leu Val Val  Ala Asp Ile 
    1145                 1150                 1155             


Lys Lys  Gly Lys Ala Gln Lys  Leu Lys Thr Val Thr  Glu Leu Leu 
    1160                 1165                 1170             


Gly Ile  Thr Ile Met Glu Arg  Ser Arg Phe Glu Lys  Asn Thr Ser 
    1175                 1180                 1185             


Ala Phe  Leu Glu Ser Lys Gly  Tyr Leu Asn Ile Arg  Asp Asp Lys 
    1190                 1195                 1200             


Leu Met  Ile Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Glu Asn Gly 
    1205                 1210                 1215             


Arg Arg  Arg Leu Leu Ala Ser  Ala Gly Glu Leu Gln  Lys Gly Asn 
    1220                 1225                 1230             


Glu Leu  Ala Leu Pro Thr Gln  Phe Met Lys Phe Leu  Tyr Leu Ala 
    1235                 1240                 1245             


Ser Arg  Tyr Asn Glu Ser Lys  Gly Lys Pro Glu Glu  Ile Glu Lys 
    1250                 1255                 1260             


Lys Gln  Glu Phe Val Asn Gln  His Val Ser Tyr Phe  Asp Asp Ile 
    1265                 1270                 1275             


Leu Gln  Leu Ile Asn Asp Phe  Ser Asn Arg Val Ile  Leu Ala Asp 
    1280                 1285                 1290             


Ala Asn  Leu Glu Lys Ile Asn  Lys Leu Tyr Gln Asp  Asn Lys Glu 
    1295                 1300                 1305             


Asn Ile  Pro Val Asp Glu Leu  Ala Asn Asn Ile Ile  Asn Leu Phe 
    1310                 1315                 1320             


Thr Phe  Thr Ser Leu Gly Ala  Pro Ala Ala Phe Lys  Phe Phe Asp 
    1325                 1330                 1335             


Lys Ile  Val Asp Arg Lys Arg  Tyr Thr Ser Thr Lys  Glu Val Leu 
    1340                 1345                 1350             


Asn Ser  Thr Leu Ile His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr 
    1355                 1360                 1365             


Arg Ile  Asp Leu Gly Lys Leu  Gly Glu Gly 
    1370                 1375             


<210>  1084
<211>  1378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1084

Met Thr Asn Glu Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Phe Ala Val Val Asn Asp Asn Asn Arg Val Ile Arg Val Lys 
            20                  25                  30          


Gly Lys Asn Ala Ile Gly Val Arg Leu Phe Asp Glu Gly Lys Ala Ala 
        35                  40                  45              


Ala Asp Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Arg Trp Arg Leu Lys Leu Leu Arg Glu Ile Phe Asp Ala Tyr Ile Thr 
65                  70                  75                  80  


Pro Val Asp Glu Ala Phe Phe Ile Arg Leu Lys Glu Ser Asn Leu Ser 
                85                  90                  95      


Pro Lys Asp Ser Lys Lys Gln Tyr Ser Gly Asp Ile Leu Phe Asn Asp 
            100                 105                 110         


Arg Ser Asp Lys Asp Phe Tyr Glu Lys Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Asn Ala Leu Met Thr Glu His Arg Lys Phe Asp Val Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Met Lys Phe Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Ala Thr Pro Ala Asn Asn Phe Lys Val Gly Arg Leu Asn Leu Glu Glu 
                165                 170                 175     


Lys Phe Glu Glu Leu Asn Asp Ile Tyr Gln Arg Val Phe Pro Asp Glu 
            180                 185                 190         


Ser Ile Glu Phe Arg Thr Asp Asn Leu Glu Gln Ile Lys Glu Val Leu 
        195                 200                 205             


Leu Asp Asn Lys Arg Ser Arg Ala Asp Arg Gln Arg Thr Leu Val Ser 
    210                 215                 220                 


Asp Ile Tyr Gln Ser Ser Glu Asp Lys Asp Ile Glu Lys Arg Asn Lys 
225                 230                 235                 240 


Ala Val Ala Thr Glu Ile Leu Lys Ala Ser Leu Gly Asn Lys Ala Lys 
                245                 250                 255     


Leu Asn Val Ile Thr Asn Val Glu Val Asp Lys Glu Ala Ala Lys Glu 
            260                 265                 270         


Trp Ser Ile Thr Phe Asp Ser Glu Ser Ile Asp Asp Asp Leu Ala Lys 
        275                 280                 285             


Ile Glu Gly Gln Met Thr Asp Asp Gly His Glu Ile Ile Glu Val Leu 
    290                 295                 300                 


Arg Ser Leu Tyr Ser Gly Ile Thr Leu Ser Ala Ile Val Pro Glu Asn 
305                 310                 315                 320 


His Thr Leu Ser Gln Ser Met Val Ala Lys Tyr Asp Leu His Lys Asp 
                325                 330                 335     


His Leu Lys Leu Phe Lys Lys Leu Ile Asn Gly Met Thr Asp Thr Lys 
            340                 345                 350         


Lys Ala Lys Asn Leu Arg Ala Ala Tyr Asp Gly Tyr Ile Asp Gly Val 
        355                 360                 365             


Lys Gly Lys Val Leu Pro Gln Glu Asp Phe Tyr Lys Gln Val Gln Val 
    370                 375                 380                 


Asn Leu Asp Asp Ser Ala Glu Ala Asn Glu Ile Gln Thr Tyr Ile Asp 
385                 390                 395                 400 


Gln Asp Ile Phe Met Pro Lys Gln Arg Thr Lys Ala Asn Gly Ser Ile 
                405                 410                 415     


Pro His Gln Leu Gln Gln Gln Glu Leu Asp Gln Ile Ile Glu Asn Gln 
            420                 425                 430         


Lys Ala Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Pro Asp Lys 
        435                 440                 445             


Lys Arg Gln Gln Leu Ala Lys Tyr Lys Leu Asp Glu Leu Val Thr Phe 
    450                 455                 460                 


Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Ala Lys Asp Gln Lys 
465                 470                 475                 480 


Asn Gln Ser Gly Ala Glu Phe Ala Trp Met Ile Arg Lys Glu Pro Gly 
                485                 490                 495     


Asn Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Met Ala Thr 
            500                 505                 510         


Ala Asn Gln Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu 
        515                 520                 525             


Gly Glu Asp Val Leu Pro Ala Gln Ser Leu Leu Tyr Gln Lys Phe Glu 
    530                 535                 540                 


Val Leu Asn Glu Leu Asn Lys Ile Arg Ile Asp His Lys Pro Ile Ser 
545                 550                 555                 560 


Ile Glu Gln Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Phe Lys 
                565                 570                 575     


Asn Val Thr Ile Lys His Leu Gln Asp Tyr Leu Val Ser Gln Gly Gln 
            580                 585                 590         


Tyr Ser Lys Arg Pro Leu Ile Glu Gly Leu Ala Asp Glu Lys Arg Phe 
        595                 600                 605             


Asn Ser Ser Leu Ser Thr Tyr Ser Asp Leu Cys Gly Ile Phe Gly Ala 
    610                 615                 620                 


Lys Leu Val Glu Glu Asn Asp Arg Gln Glu Asp Leu Glu Lys Ile Ile 
625                 630                 635                 640 


Glu Trp Ser Thr Ile Phe Glu Asp Lys Lys Ile Tyr Arg Ala Lys Leu 
                645                 650                 655     


Asn Asp Leu Thr Trp Leu Thr Asp Asp Gln Lys Glu Lys Leu Ala Thr 
            660                 665                 670         


Lys Arg Tyr Gln Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Val Gly 
        675                 680                 685             


Leu Lys Asn Ser Glu His Arg Asn Ile Met Asp Ile Leu Trp Ile Thr 
    690                 695                 700                 


Asn Glu Asn Phe Met Gln Ile Gln Ala Glu Pro Asp Phe Ala Lys Leu 
705                 710                 715                 720 


Val Thr Asp Ala Asn Lys Gly Met Leu Glu Lys Thr Asp Ser Gln Asp 
                725                 730                 735     


Val Ile Asn Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Ile Leu Leu Val Val His Asp Ile Gln Asn Ala Met His Gly Gln 
        755                 760                 765             


Ala Pro Ala Lys Ile His Val Glu Phe Ala Arg Gly Glu Glu Arg Asn 
    770                 775                 780                 


Pro Arg Arg Ser Val Gln Arg Gln Arg Gln Val Glu Ala Ala Tyr Glu 
785                 790                 795                 800 


Lys Val Ser Asn Glu Leu Val Ser Ala Lys Val Arg Gln Glu Phe Lys 
                805                 810                 815     


Glu Ala Ile Asn Asn Lys Arg Asp Phe Lys Asp Arg Leu Phe Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Ile Asp Ile Tyr Thr Gly Lys Gln Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser Ser Tyr Gln Ile Asp His Ile Leu Pro Gln Ala Phe 
    850                 855                 860                 


Val Lys Asp Asp Ser Leu Thr Asn Arg Val Leu Thr Asn Glu Asn Gln 
865                 870                 875                 880 


Val Lys Ala Asp Ser Val Pro Ile Asp Ile Phe Gly Lys Lys Met Leu 
                885                 890                 895     


Ser Val Trp Gly Arg Met Lys Asp Gln Gly Leu Ile Ser Lys Gly Lys 
            900                 905                 910         


Tyr Arg Asn Leu Thr Met Asn Pro Glu Asn Ile Ser Ala His Thr Glu 
        915                 920                 925             


Asn Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val Ile Lys 
    930                 935                 940                 


Leu Ala Val Asn Ile Leu Ala Asp Glu Tyr Gly Asp Ser Thr Gln Ile 
945                 950                 955                 960 


Ile Ser Val Lys Ala Asp Leu Ser His Gln Met Arg Glu Asp Phe Glu 
                965                 970                 975     


Leu Leu Lys Asn Arg Asp Val Asn Asp Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Tyr Leu Ala Ala Phe Ile Gly Asn  Tyr Leu Leu Lys Arg  Tyr Pro Lys 
        995                 1000                 1005             


Leu Glu  Ser Tyr Phe Val Tyr  Gly Asp Phe Lys Lys  Phe Thr Gln 
    1010                 1015                 1020             


Lys Glu  Thr Lys Met Arg Arg  Phe Asn Phe Ile Tyr  Asp Leu Lys 
    1025                 1030                 1035             


His Cys  Asp Gln Val Val Asn  Lys Glu Thr Gly Glu  Ile Leu Trp 
    1040                 1045                 1050             


Thr Lys  Asp Glu Asp Ile Lys  Tyr Ile Arg His Ser  Phe Ala Tyr 
    1055                 1060                 1065             


Lys Lys  Ile Leu Val Ser His  Glu Val Arg Glu Lys  Arg Gly Ala 
    1070                 1075                 1080             


Leu Tyr  Asn Gln Thr Ile Tyr  Lys Ala Lys Asp Asp  Lys Gly Ser 
    1085                 1090                 1095             


Gly Gln  Gly Ser Lys Lys Leu  Ile Arg Ile Lys Asp  Asp Lys Glu 
    1100                 1105                 1110             


Thr Lys  Ile Tyr Gly Gly Tyr  Ser Gly Lys Ser Leu  Ala Tyr Met 
    1115                 1120                 1125             


Thr Ile  Val Gln Ile Thr Lys  Lys Asn Lys Val Ser  Tyr Arg Val 
    1130                 1135                 1140             


Ile Gly  Ile Pro Thr Leu Ala  Leu Ala Arg Leu Asn  Lys Leu Glu 
    1145                 1150                 1155             


Asn Asp  Ser Thr Glu Asn Asn  Gly Glu Leu Tyr Lys  Ile Ile Lys 
    1160                 1165                 1170             


Pro Gln  Phe Thr His Tyr Lys  Val Asp Lys Lys Asn  Gly Glu Ile 
    1175                 1180                 1185             


Ile Glu  Thr Thr Asp Asp Phe  Lys Ile Val Val Ser  Lys Val Arg 
    1190                 1195                 1200             


Phe Gln  Gln Leu Ile Asp Asp  Ala Gly Gln Phe Phe  Met Leu Ala 
    1205                 1210                 1215             


Ser Asp  Thr Tyr Lys Asn Asn  Ala Gln Gln Leu Val  Ile Ser Asn 
    1220                 1225                 1230             


Asn Ala  Leu Lys Ala Ile Asn  Asn Thr Asn Ile Thr  Asp Cys Pro 
    1235                 1240                 1245             


Arg Asp  Asp Leu Glu Arg Leu  Asp Asn Leu Arg Leu  Asp Ser Ala 
    1250                 1255                 1260             


Phe Asp  Glu Ile Val Lys Lys  Met Asp Lys Tyr Phe  Ser Ala Tyr 
    1265                 1270                 1275             


Asp Ala  Asn Asn Phe Arg Glu  Lys Ile Arg Asn Ser  Asn Leu Ile 
    1280                 1285                 1290             


Phe Tyr  Gln Leu Pro Val Glu  Asp Gln Trp Glu Asn  Asn Lys Ile 
    1295                 1300                 1305             


Thr Glu  Leu Gly Lys Arg Thr  Val Leu Thr Arg Ile  Leu Gln Gly 
    1310                 1315                 1320             


Leu His  Ala Asn Ala Thr Thr  Thr Asp Met Ser Ile  Phe Lys Ile 
    1325                 1330                 1335             


Lys Thr  Pro Phe Gly Gln Leu  Arg Gln Arg Ser Gly  Ile Ser Leu 
    1340                 1345                 1350             


Ser Glu  Asn Ala Gln Leu Ile  Tyr Gln Ser Pro Thr  Gly Leu Phe 
    1355                 1360                 1365             


Glu Arg  Arg Val Gln Leu Asn  Lys Ile Lys 
    1370                 1375             


<210>  1085
<211>  1378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1085

Met Thr Asn Glu Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Ile Gly Phe Ala Val Val Asn Asp Asn Asn Arg Val Ile Arg Val Lys 
            20                  25                  30          


Gly Lys Asn Ala Ile Gly Val Arg Leu Phe Asp Glu Gly Lys Ala Ala 
        35                  40                  45              


Ala Asp Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
    50                  55                  60                  


Arg Trp Arg Leu Lys Leu Leu Arg Glu Ile Phe Asp Ala Tyr Ile Thr 
65                  70                  75                  80  


Pro Val Asp Glu Ala Phe Phe Ile Arg Leu Lys Glu Ser Asn Leu Ser 
                85                  90                  95      


Pro Lys Asp Ser Lys Lys Gln Tyr Ser Gly Asp Ile Leu Phe Asn Asp 
            100                 105                 110         


Arg Ser Asp Lys Asp Phe Tyr Glu Lys Tyr Pro Thr Ile Tyr His Leu 
        115                 120                 125             


Arg Asn Ala Leu Met Thr Glu His Arg Lys Phe Asp Val Arg Glu Ile 
    130                 135                 140                 


Tyr Leu Ala Ile His His Ile Met Lys Phe Arg Gly His Phe Leu Asn 
145                 150                 155                 160 


Ala Thr Pro Ala Asn Asn Phe Lys Val Gly Arg Leu Asn Leu Glu Glu 
                165                 170                 175     


Lys Phe Glu Glu Leu Asn Asp Ile Tyr Gln Arg Val Phe Pro Asp Glu 
            180                 185                 190         


Ser Ile Glu Phe Arg Thr Asp Asn Leu Glu Gln Ile Lys Glu Val Leu 
        195                 200                 205             


Leu Asp Asn Lys Arg Ser Arg Ala Asp Arg Gln Arg Thr Leu Val Ser 
    210                 215                 220                 


Asp Ile Tyr Gln Ser Ser Glu Asp Lys Asp Ile Glu Lys Arg Asn Lys 
225                 230                 235                 240 


Ala Val Ala Thr Glu Ile Leu Lys Ala Ser Leu Gly Asn Lys Ala Lys 
                245                 250                 255     


Leu Asn Val Ile Thr Asn Val Glu Val Asp Lys Glu Ala Ala Lys Glu 
            260                 265                 270         


Trp Ser Ile Thr Phe Asp Ser Glu Ser Ile Asp Asp Asp Leu Ala Lys 
        275                 280                 285             


Ile Glu Gly Gln Met Thr Asp Asp Gly His Glu Ile Ile Glu Val Leu 
    290                 295                 300                 


Arg Ser Leu Tyr Ser Gly Ile Thr Leu Ser Ala Ile Val Pro Glu Asn 
305                 310                 315                 320 


His Thr Leu Ser Gln Ser Met Val Ala Lys Tyr Asp Leu His Lys Asp 
                325                 330                 335     


His Leu Lys Leu Phe Lys Lys Leu Ile Asn Gly Met Thr Asp Thr Lys 
            340                 345                 350         


Lys Ala Lys Asn Leu Arg Ala Ala Tyr Asp Gly Tyr Ile Asp Gly Val 
        355                 360                 365             


Lys Gly Lys Val Leu Pro Gln Glu Asp Phe Tyr Lys Gln Val Gln Val 
    370                 375                 380                 


Asn Leu Asp Asp Ser Ala Glu Ala Asn Glu Ile Gln Thr Tyr Ile Asp 
385                 390                 395                 400 


Gln Asp Ile Phe Met Pro Lys Gln Arg Thr Lys Ala Asn Gly Ser Ile 
                405                 410                 415     


Pro His Gln Leu Gln Gln Gln Glu Leu Asp Gln Ile Ile Glu Asn Gln 
            420                 425                 430         


Lys Ala Tyr Tyr Pro Trp Leu Ala Glu Leu Asn Pro Asn Pro Asp Lys 
        435                 440                 445             


Lys Arg Gln Gln Leu Ala Lys Tyr Lys Leu Asp Glu Leu Val Thr Phe 
    450                 455                 460                 


Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Ala Lys Asp Gln Lys 
465                 470                 475                 480 


Asn Gln Ser Gly Ala Glu Phe Ala Trp Met Ile Arg Lys Glu Pro Gly 
                485                 490                 495     


Asn Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Met Ala Thr 
            500                 505                 510         


Ala Asn Gln Phe Ile Lys Arg Met Thr Thr Thr Asp Thr Tyr Leu Leu 
        515                 520                 525             


Gly Glu Asp Val Leu Pro Ala Gln Ser Leu Leu Tyr Gln Lys Phe Glu 
    530                 535                 540                 


Val Leu Asn Glu Leu Asn Lys Ile Arg Ile Asp His Lys Pro Ile Ser 
545                 550                 555                 560 


Ile Glu Gln Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Phe Lys 
                565                 570                 575     


Asn Val Thr Ile Lys His Leu Gln Asp Tyr Leu Val Ser Gln Gly Gln 
            580                 585                 590         


Tyr Ser Lys Arg Pro Leu Ile Glu Gly Leu Ala Asp Glu Lys Arg Phe 
        595                 600                 605             


Asn Ser Ser Leu Ser Thr Tyr Ser Asp Leu Cys Gly Ile Phe Gly Ala 
    610                 615                 620                 


Lys Leu Val Glu Glu Asn Asp Arg Gln Glu Asp Leu Glu Lys Ile Ile 
625                 630                 635                 640 


Glu Trp Ser Thr Ile Phe Glu Asp Lys Lys Ile Tyr Arg Ala Lys Leu 
                645                 650                 655     


Asn Asp Leu Thr Trp Leu Thr Asp Asp Gln Lys Glu Lys Leu Ala Thr 
            660                 665                 670         


Lys Arg Tyr Gln Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Val Gly 
        675                 680                 685             


Leu Lys Asn Ser Glu His Arg Asn Ile Met Asp Ile Leu Trp Ile Thr 
    690                 695                 700                 


Asn Glu Asn Phe Met Gln Ile Gln Ala Glu Pro Asp Phe Ala Lys Leu 
705                 710                 715                 720 


Val Thr Asp Ala Asn Lys Gly Met Leu Glu Lys Thr Asp Ser Gln Asp 
                725                 730                 735     


Val Ile Asn Asp Leu Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
            740                 745                 750         


Gln Ile Leu Leu Val Val His Asp Ile Gln Asn Ala Met His Gly Gln 
        755                 760                 765             


Ala Pro Ala Lys Ile His Val Glu Phe Ala Arg Gly Glu Glu Arg Asn 
    770                 775                 780                 


Pro Arg Arg Ser Val Gln Arg Gln Arg Gln Val Glu Ala Ala Tyr Glu 
785                 790                 795                 800 


Lys Val Ser Asn Glu Leu Val Ser Ala Lys Val Arg Gln Glu Phe Lys 
                805                 810                 815     


Glu Ala Ile Asn Asn Lys Arg Asp Phe Lys Asp Arg Leu Phe Leu Tyr 
            820                 825                 830         


Phe Met Gln Gly Gly Ile Asp Ile Tyr Thr Gly Lys Gln Leu Asn Ile 
        835                 840                 845             


Asp Gln Leu Ser Ser Tyr Gln Ile Asp His Ile Leu Pro Gln Ala Phe 
    850                 855                 860                 


Val Lys Asp Asp Ser Leu Thr Asn Arg Val Leu Thr Asn Glu Asn Gln 
865                 870                 875                 880 


Val Lys Ala Asp Ser Val Pro Ile Asp Ile Phe Gly Lys Lys Met Leu 
                885                 890                 895     


Ser Val Trp Gly Arg Met Lys Asp Gln Gly Leu Ile Ser Lys Gly Lys 
            900                 905                 910         


Tyr Arg Asn Leu Thr Met Asn Pro Glu Asn Ile Ser Ala His Thr Glu 
        915                 920                 925             


Asn Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Val Ile Lys 
    930                 935                 940                 


Leu Ala Val Asn Ile Leu Ala Asp Glu Tyr Gly Asp Ser Thr Gln Ile 
945                 950                 955                 960 


Ile Ser Val Lys Ala Asp Leu Ser His Gln Met Arg Glu Asp Phe Glu 
                965                 970                 975     


Leu Leu Lys Asn Arg Asp Val Asn Asp Tyr His His Ala Phe Asp Ala 
            980                 985                 990         


Tyr Leu Ala Ala Phe Ile Gly Asn  Tyr Leu Leu Lys Arg  Tyr Pro Lys 
        995                 1000                 1005             


Leu Glu  Ser Tyr Phe Val Tyr  Gly Asp Phe Lys Lys  Phe Thr Gln 
    1010                 1015                 1020             


Lys Glu  Thr Lys Met Arg Arg  Phe Asn Phe Ile Tyr  Asp Leu Lys 
    1025                 1030                 1035             


His Cys  Asp Gln Val Val Asn  Lys Glu Thr Gly Glu  Ile Leu Trp 
    1040                 1045                 1050             


Thr Lys  Asp Glu Asp Ile Lys  Tyr Ile Arg His Leu  Phe Ala Tyr 
    1055                 1060                 1065             


Lys Lys  Ile Leu Val Ser His  Glu Val Arg Glu Lys  Arg Gly Ala 
    1070                 1075                 1080             


Leu Tyr  Asn Gln Thr Ile Tyr  Lys Ala Lys Asp Asp  Lys Gly Ser 
    1085                 1090                 1095             


Gly Gln  Gly Ser Lys Lys Leu  Ile Arg Ile Lys Asp  Asp Lys Glu 
    1100                 1105                 1110             


Thr Lys  Ile Tyr Gly Gly Tyr  Ser Gly Lys Ser Leu  Ala Tyr Met 
    1115                 1120                 1125             


Thr Ile  Val Gln Ile Thr Lys  Lys Asn Lys Val Ser  Tyr Arg Val 
    1130                 1135                 1140             


Ile Gly  Ile Pro Thr Leu Ala  Leu Ala Arg Leu Asn  Lys Leu Glu 
    1145                 1150                 1155             


Asn Asp  Ser Thr Glu Asn Asn  Gly Glu Leu Tyr Lys  Ile Ile Lys 
    1160                 1165                 1170             


Pro Gln  Phe Thr His Tyr Lys  Val Asp Lys Lys Asn  Gly Glu Ile 
    1175                 1180                 1185             


Ile Glu  Thr Thr Asp Asp Phe  Lys Ile Val Val Ser  Lys Val Arg 
    1190                 1195                 1200             


Phe Gln  Gln Leu Ile Asp Asp  Ala Gly Gln Phe Phe  Met Leu Ala 
    1205                 1210                 1215             


Ser Asp  Thr Tyr Lys Asn Asn  Ala Gln Gln Leu Val  Ile Ser Asn 
    1220                 1225                 1230             


Asn Ala  Leu Lys Ala Ile Asn  Asn Thr Asn Ile Thr  Asp Cys Pro 
    1235                 1240                 1245             


Arg Asp  Asp Leu Glu Arg Leu  Asp Asn Leu Arg Leu  Asp Ser Ala 
    1250                 1255                 1260             


Phe Asp  Glu Ile Val Lys Lys  Met Asp Lys Tyr Phe  Ser Ala Tyr 
    1265                 1270                 1275             


Asp Ala  Asn Asn Phe Arg Glu  Lys Ile Arg Asn Ser  Asn Leu Ile 
    1280                 1285                 1290             


Phe Tyr  Gln Leu Pro Val Glu  Asp Gln Trp Glu Asn  Asn Lys Ile 
    1295                 1300                 1305             


Thr Glu  Leu Gly Lys Arg Thr  Val Leu Thr Arg Ile  Leu Gln Gly 
    1310                 1315                 1320             


Leu His  Ala Asn Ala Thr Thr  Thr Asp Met Ser Ile  Phe Lys Ile 
    1325                 1330                 1335             


Lys Thr  Pro Phe Gly Gln Leu  Arg Gln Arg Ser Gly  Ile Ser Leu 
    1340                 1345                 1350             


Ser Glu  Asn Ala Gln Leu Ile  Tyr Gln Ser Pro Thr  Gly Leu Phe 
    1355                 1360                 1365             


Glu Arg  Arg Val Gln Leu Asn  Lys Ile Lys 
    1370                 1375             


<210>  1086
<211>  1379
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1086

Met Lys Gln Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Arg Glu Ala Glu Asn Lys Asp Glu Gln Ser Ser Ile Val 
            20                  25                  30          


Arg Ile Gly Val Arg Val Asn Pro Leu Thr Ser Asp Glu Gln Lys Asn 
        35                  40                  45              


Phe Glu Gln Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Asp His 
65                  70                  75                  80  


Leu Lys Asp Cys Leu Gln Lys Asn Gly Ile Ile Thr Asp Lys Ala Val 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Tyr Thr Thr Phe Gln Thr Tyr Tyr Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Asn Glu Lys Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Met 
    130                 135                 140                 


Lys Ser Ala Asp Glu Gly Gln Leu Ile Asp Gly Met Ser Val Ala Lys 
145                 150                 155                 160 


Arg Leu Tyr Asn Asp Asn Ile Thr Pro Gly Gln Tyr Val Leu Ser Leu 
                165                 170                 175     


Leu Glu Glu Gly Arg Lys Tyr Ile Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Leu Asp Glu Leu Asp Lys Ile Trp Thr Phe Gln Gln Cys Phe Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Pro Glu Cys Arg Lys Leu Ile Ala Gly Lys Gly Lys 
    210                 215                 220                 


Gln Ala Thr Ala Lys Ile Phe Leu Gly Lys Tyr Lys Ile Tyr Thr Ala 
225                 230                 235                 240 


Asp Asn Lys Ser Lys Asp Lys Arg Leu Gln Ala Tyr Gln Trp Arg Val 
                245                 250                 255     


Glu Ala Leu Ser Gly Gln Leu Pro Ile Glu Val Val Ala Phe Val Val 
            260                 265                 270         


Cys Glu Ile Asn Gly Ala Ile Gln Ala Ser Ser Gly Tyr Leu Gly Ala 
        275                 280                 285             


Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Glu Gly Leu Thr Val Gly 
    290                 295                 300                 


Gln Tyr Leu Val Lys Gln Leu Glu Lys Asn Pro Asp Thr Ser Leu Lys 
305                 310                 315                 320 


Asn Lys Val Phe Tyr Arg Gln Asp Tyr Leu Asn Glu Phe Glu Lys Ile 
                325                 330                 335     


Trp Glu Lys Gln Ala Glu Tyr His Thr Glu Leu Thr Pro Gln Leu Lys 
            340                 345                 350         


Lys Glu Val Arg Asp Ile Ile Ile Phe Tyr Gln Arg Thr Leu Lys Ser 
        355                 360                 365             


Gln Lys Gly Leu Val Gly Phe Cys Glu Phe Glu Ser Arg Gln Ile Glu 
    370                 375                 380                 


Val Met Ile Asp Gly Lys Leu Lys Lys Lys Thr Val Gly Cys Arg Val 
385                 390                 395                 400 


Cys Pro Lys Ser Ser Pro Leu Phe Gln Glu Tyr Lys Ile Trp His Asn 
                405                 410                 415     


Leu Asn Asn Leu Lys Ile Val Glu Lys Glu Leu Gly Lys Ser Arg Phe 
            420                 425                 430         


Leu Asn Gln Asp Glu Lys Glu Ser Leu Phe Gln Glu Leu Asn Ile Lys 
        435                 440                 445             


Glu Lys Leu Ser Lys Thr Asp Ile Leu Lys Leu Leu Cys Leu Lys Ala 
    450                 455                 460                 


Lys Glu Phe Asp Leu Asn Phe Glu Lys Val Glu Gly Asn Arg Thr Gln 
465                 470                 475                 480 


Ala Glu Leu Phe Arg Ala Tyr Gln Ala Ile Ile Glu Met Ser Gly Asn 
                485                 490                 495     


Gly Asp Tyr Asp Phe Ser Lys Met Thr Ser Ser Glu Ile Gln Asp Thr 
            500                 505                 510         


Val Thr Gly Val Phe Arg Gly Leu Gly Tyr Asn Thr Asp Ile Leu Ser 
        515                 520                 525             


Phe Asp Ala Glu Lys Glu Glu Lys Thr Phe Asp Lys Gln Pro Met Tyr 
    530                 535                 540                 


Leu Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Ser Ser Asp Thr 
545                 550                 555                 560 


Gly Asn Lys Ala Leu Ile Thr Lys Ile Lys Lys Leu Cys Gly Phe Glu 
                565                 570                 575     


Ser Glu Glu Tyr Ala Arg Ile Leu Thr Asp Val Thr Phe Gln Ser Glu 
            580                 585                 590         


Tyr Gly Asn Leu Ser Thr Lys Ala Ile Arg Arg Ile Leu Pro Phe Met 
        595                 600                 605             


Lys Lys Gly Ser Glu Tyr Ser Glu Ala Cys Arg His Ala Gly Tyr Arg 
    610                 615                 620                 


His Ser Glu Arg Ser Leu Thr Lys Glu Glu Leu Asp Lys Lys Val Tyr 
625                 630                 635                 640 


Lys Asp His Leu Glu Leu Leu Pro Arg Asn Ser Leu Arg Asn Pro Val 
                645                 650                 655     


Val Glu Lys Ile Leu Asn Gln Met Ile His Val Val Asn Thr Val Val 
            660                 665                 670         


Asp Thr Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu 
        675                 680                 685             


Leu Lys Lys Ser Ala Lys Glu Arg Asp Glu Met Thr Ala Ser Ile Asn 
    690                 695                 700                 


Lys Thr Ala Ala Glu His Lys Lys Tyr Cys Glu Val Leu Gln Lys Glu 
705                 710                 715                 720 


Phe Gly Phe Thr His Val Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu 
                725                 730                 735     


Tyr Leu Glu Leu Glu Asn Asn Gly Tyr Lys Thr Leu Tyr Ser Asp Thr 
            740                 745                 750         


Tyr Ile Pro Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His 
        755                 760                 765             


Ile Ile Pro Gln Ser Lys Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr 
    770                 775                 780                 


Leu Glu Cys Arg Ser Val Asn Ile Glu Lys Ser Asn Asp Thr Ala Tyr 
785                 790                 795                 800 


Asp Tyr Val Leu Asn Lys Tyr Gly Glu Gln Gly Ile Leu Asp Tyr Lys 
                805                 810                 815     


Asn Arg Ile Glu Asn Leu Tyr Lys Asp Ser Lys Ile Ser Arg Thr Lys 
            820                 825                 830         


Tyr Gln Lys Leu Met Met Lys Asn Ser Asp Ile Ser Gly Asn Phe Ile 
        835                 840                 845             


Glu Arg Glu Leu Arg Asp Ser Gln Tyr Ile Ala Lys Lys Ala Arg Glu 
    850                 855                 860                 


Ile Leu Glu Glu Leu Val Lys Phe Val Val Pro Thr Thr Gly Ser Val 
865                 870                 875                 880 


Thr Ala Arg Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Gln Glu 
                885                 890                 895     


Leu Asn Trp Lys Lys Tyr Asp Lys Leu Gly Leu Thr Asp Val Arg Tyr 
            900                 905                 910         


Asp Lys Asp Gly Arg Glu Ile Arg Tyr Ile Lys Asp Trp Ser Lys Arg 
        915                 920                 925             


Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr 
    930                 935                 940                 


Lys Arg Ser Ile Ile Gln Tyr Leu Asn Asn Leu Asn Ala Lys Ser Asp 
945                 950                 955                 960 


Lys Ser Gly Ser Ile Tyr Ala Ile Glu Gln Lys Glu Leu Phe Arg Asp 
                965                 970                 975     


Lys Arg Gly Lys Leu Lys Phe Ile Pro Pro Met Pro Cys Asn Ile Phe 
            980                 985                 990         


Arg Ala Glu Ala Lys Arg His Leu  Glu Asn Val Leu Val  Ser Val Lys 
        995                 1000                 1005             


Ala Lys  Asn Lys Val Val Thr  Pro Asn Ile Asn Arg  Ser Lys Gly 
    1010                 1015                 1020             


Lys Asn  Gly Ile Asn Lys Lys  Ile Gln Leu Thr Pro  Arg Gly Gln 
    1025                 1030                 1035             


Leu His  Asn Glu Thr Ile Tyr  Gly Ser Met Lys Arg  Tyr Glu Thr 
    1040                 1045                 1050             


Lys Trp  Glu Lys Ile Gly Ser  Gly Phe Asp Glu Arg  Gln Ile Ala 
    1055                 1060                 1065             


Lys Val  Ala Asn Lys Lys Phe  Arg Glu Ala Leu Leu  Ser Arg Leu 
    1070                 1075                 1080             


Ala Glu  Phe Gly Asn Asp Pro  Gln Lys Ala Phe Thr  Gly Lys Asn 
    1085                 1090                 1095             


Ser Leu  Glu Lys Asn Pro Leu  Tyr Val Asp Glu Leu  His Thr Gln 
    1100                 1105                 1110             


Lys Val  Pro Glu Lys Val Lys  Ile Val Thr Leu Glu  Asn Leu Tyr 
    1115                 1120                 1125             


Thr Ile  Arg Lys Glu Ile Thr  Pro Asp Leu Lys Ile  Asp Lys Val 
    1130                 1135                 1140             


Val Asp  Ala Gly Val Arg Arg  Val Leu Gln Glu Arg  Leu Asp Glu 
    1145                 1150                 1155             


Phe Asp  Asp Asp Ser Lys Lys  Ala Phe Ser Asn Leu  Asp Glu Asn 
    1160                 1165                 1170             


Pro Ile  Trp Leu Asn Gly Lys  Lys Met Lys Arg Val  Thr Ile Met 
    1175                 1180                 1185             


Gly Val  Ser Asn Ala Val Pro  Leu His Asp Lys Lys  Asp Tyr Lys 
    1190                 1195                 1200             


Gly Asn  Leu Val Phe Asp Ser  Lys Gly Arg Lys Gln  Pro Val Asp 
    1205                 1210                 1215             


Phe Val  Asn Thr Gly Ser Asn  His His Val Ala Ile  Tyr Arg Asp 
    1220                 1225                 1230             


Glu Ser  Gly Glu Leu Gln Asp  Ile Val Val Ser Phe  Tyr Glu Ala 
    1235                 1240                 1245             


Thr Thr  Arg Ser Thr Leu Gly  Ile Pro Val Ile Asp  Lys Glu Tyr 
    1250                 1255                 1260             


Lys Lys  Ala Glu Gly Trp Asn  Phe Leu Phe Ser Met  Lys Gln Asn 
    1265                 1270                 1275             


Glu Tyr  Phe Val Phe Pro Asn  Glu Glu Thr Gly Phe  Asn Pro Lys 
    1280                 1285                 1290             


Glu Ile  Asp Leu Met Asn Pro  Asp Asn Tyr Thr Thr  Ile Ser Pro 
    1295                 1300                 1305             


Asn Leu  Tyr Arg Val Gln Lys  Phe Ser Lys Cys Met  Tyr Gly Asn 
    1310                 1315                 1320             


Ser Ser  Val Arg Glu Tyr Val  Phe Arg His His Leu  Glu Thr Ser 
    1325                 1330                 1335             


Val Asn  Asp Ser Lys Gly Leu  Lys Asn Ile Ala Tyr  Lys Asn Val 
    1340                 1345                 1350             


Lys Ser  Leu Val Tyr Leu Glu  Gly Ile Val Lys Val  Arg Ile Asn 
    1355                 1360                 1365             


His Ile  Gly Gln Ile Val Ser  Val Gly Glu Tyr 
    1370                 1375                 


<210>  1087
<211>  1379
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1087

Met Arg Ile Asp Asn Gly Leu Glu Glu Leu Lys Val Asn Asn Arg Ile 
1               5                   10                  15      


Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser Val Gly Phe Ala Val 
            20                  25                  30          


Thr Asp Ala Gln Asp Lys Leu Val Arg Val Lys Gly Lys Thr Ala Ile 
        35                  40                  45              


Gly Val Arg Leu Phe Glu Glu Gly His Thr Ala Ala Glu Arg Arg Gly 
    50                  55                  60                  


Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg Lys Trp Arg Ile Asn 
65                  70                  75                  80  


Leu Leu Asn Gln Ile Phe Glu Pro Tyr Ile Ser Glu Ile Asp Pro Thr 
                85                  90                  95      


Phe Phe Ala Arg Leu Lys Asn Ser Ser Leu Ala Gly Lys Asp Lys Lys 
            100                 105                 110         


Phe Glu Gly Ser Leu Leu Phe Pro Asp Arg Thr Asp Tyr Gln Phe Tyr 
        115                 120                 125             


Lys Asp Asn Pro Thr Met Tyr His Leu Arg Lys His Leu Met Glu Lys 
    130                 135                 140                 


Asp Arg Gln Phe Asp Leu Arg Glu Ile Tyr Leu Ala Met His His Ile 
145                 150                 155                 160 


Ile Lys Tyr Arg Gly Asn Phe Leu Asn Thr Thr Pro Met Asn His Phe 
                165                 170                 175     


Lys Asn Glu Glu Ile Asp Phe Ser Ala Leu Phe Ser Arg Leu Asn Glu 
            180                 185                 190         


Leu Tyr Gln Glu Ile Asp Asp Glu Asn Ile Phe Glu Leu Asn Ile Glu 
        195                 200                 205             


Asn Val Asp Glu Ile Lys Lys Ile Leu Leu Asn His Asp Leu Phe Lys 
    210                 215                 220                 


Met Glu Lys Gln Lys Gln Val Ala Lys Leu Leu Asn Val Asp Thr Asp 
225                 230                 235                 240 


Asp Lys Gln Leu Lys Lys Glu Asn Thr Ala Leu Ala Ser Asn Phe Ser 
                245                 250                 255     


Lys Ala Ile Leu Gly Tyr Lys Phe Asn Leu Ala Leu Val Thr Lys Ile 
            260                 265                 270         


Pro Asp Gln Asp Lys Lys Glu Trp Thr Ile Ser Leu Gly Asp Glu Asp 
        275                 280                 285             


Phe Asp Asp Arg Leu Asp Asn Val Pro Val Ala Leu Thr Glu Ser Gln 
    290                 295                 300                 


Leu Glu Val Val Glu Val Leu His Ser Ile Phe Asn Ser Ile Ser Leu 
305                 310                 315                 320 


Asn Glu Ile Val Glu Asn Gly Glu Thr Leu Ser Ser Ala Met Val Arg 
                325                 330                 335     


Lys Tyr Asn Asp His Lys His Asp Leu Lys Leu Leu Lys Asn Val Met 
            340                 345                 350         


Thr Thr Leu Glu Asp Arg Lys Lys Ala Lys Lys Ile Gln Gly Ile Tyr 
        355                 360                 365             


Asn Val Phe Val Gly Lys Asp Thr Asp Leu Ile Ser Asp Lys Asp Lys 
    370                 375                 380                 


Asn Lys Tyr Lys Asp Lys Asp Glu Phe Tyr Lys Glu Ile Gln Lys Asn 
385                 390                 395                 400 


Leu Asp Asn Ser Ser Asp Ala Glu Glu Ile Ile Lys Arg Ile Lys Gln 
                405                 410                 415     


Asp Gln Phe Leu Pro Lys Gln Arg Thr Ser Lys Asn Gly Val Ile Pro 
            420                 425                 430         


His Gln Leu His Gln Arg Glu Leu Asp Leu Ile Ile Glu Asn Gln Ala 
        435                 440                 445             


Lys Tyr Tyr Pro Phe Leu Lys Glu Ala Asn Pro Asn Lys Lys Arg Leu 
    450                 455                 460                 


Lys Gln Ala Lys Tyr Lys Leu Asp Glu Leu Ile Ala Phe Lys Ile Pro 
465                 470                 475                 480 


Tyr Tyr Ile Gly Pro Thr Ser Gly Ala Glu Asn Ser Asp Phe Ser Trp 
                485                 490                 495     


Leu Lys Arg Lys Lys Asp Gly Gln Ile Thr Pro Trp Asn Phe Asp Glu 
            500                 505                 510         


Lys Val Asp Arg Ile Glu Ser Ala Asn Arg Phe Ile Arg Arg Met Thr 
        515                 520                 525             


Thr Lys Asp Thr Tyr Leu Leu Ala Glu Asp Val Leu Pro Leu Glu Ser 
    530                 535                 540                 


Leu Ile Tyr Glu Lys Tyr Ser Val Leu Asn Glu Leu Asn Asn Val Arg 
545                 550                 555                 560 


Ile Asn Arg Gln Lys Leu Ser Val Asn Asp Lys Gln Asp Ile Tyr Asn 
                565                 570                 575     


Glu Leu Phe Lys Asn Ser Lys Thr Val Thr Leu Lys Gln Leu Gln Lys 
            580                 585                 590         


Tyr Phe Glu Val Thr Lys Lys Leu Lys Thr Val Val Val Glu Gly Leu 
        595                 600                 605             


Ala Asp Pro Lys Lys Phe Leu Ser Asn Leu Gly Thr Tyr His Asp Phe 
    610                 615                 620                 


Lys Leu Ile Phe Gly Asp Ile Val Asp Asp Pro Glu Tyr Gln Thr Asp 
625                 630                 635                 640 


Leu Glu Asn Ile Ile Glu Trp Ser Thr Ile Phe Glu Asp Arg Gly Ile 
                645                 650                 655     


Phe Asn Ser Lys Leu Asn Glu Ile Thr Trp Leu Thr Asp Glu Gln Arg 
            660                 665                 670         


Lys Lys Val Leu Ser Lys Arg Tyr Gln Gly Trp Gly Arg Phe Ser Lys 
        675                 680                 685             


Lys Leu Leu Val Gly Leu Lys Asn Ser Gln Gly Asn Ser Ile Leu Asp 
    690                 695                 700                 


Glu Leu Trp Asn Thr Asn Leu Asn Phe Met Gln Leu Gln Ser Gln Asp 
705                 710                 715                 720 


Asp Phe Ala Glu Leu Ile His Ala Glu Asn Ala Lys Gln Phe Lys Ala 
                725                 730                 735     


Asn Asp Pro Asp Asn Pro Trp Asn Gly Ile Asp Ala Ile Leu Asn Asp 
            740                 745                 750         


Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Val Lys 
        755                 760                 765             


Val Val Gln Asp Ile Glu Lys Ala Met Gly Asn Lys Pro Gln Lys Ile 
    770                 775                 780                 


Ala Ile Glu Phe Thr Arg Ser Ala Asp Lys Asn Pro Arg Arg Thr Asp 
785                 790                 795                 800 


Ser Arg Ile Lys Arg Leu Ser Asp Ile Tyr Lys Glu Ala Ala Glu Lys 
                805                 810                 815     


Val Val Gly Glu Ser Leu Lys Leu Glu Leu Asn Glu Tyr Leu Thr Ser 
            820                 825                 830         


Lys Lys Lys Met Thr Asp Lys Met Tyr Leu Tyr Phe Thr Gln Leu Gly 
        835                 840                 845             


Thr Asp Ile Tyr Thr Gly Lys Lys Ile Asp Ile Asp Gln Leu Asn Asn 
    850                 855                 860                 


Tyr Asp Ile Asp His Val Leu Pro Gln Ser Phe Leu Lys Asp Asp Ser 
865                 870                 875                 880 


Leu Asp Asn Arg Val Leu Thr Asn Arg Ser Asp Asn Ser Glu Lys Ser 
                885                 890                 895     


Asp Thr Val Pro Leu His Lys Phe Gly Ala Lys Met Gly Asn Phe Trp 
            900                 905                 910         


Arg Gln Leu Ala Asp Glu Gly Leu Ile Thr Lys Arg Lys Tyr Lys Asn 
        915                 920                 925             


Leu Thr Thr Asp Pro Ala Thr Ile Asp Lys Tyr Thr Ile Gln Gly Phe 
    930                 935                 940                 


Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Thr Ala 
945                 950                 955                 960 


Asn Ile Leu Gly Gly Leu Tyr Pro Glu Glu Thr Glu Ile Leu Glu Ile 
                965                 970                 975     


Pro Ala Lys Leu Asn His Gln Phe Arg Asp Met Phe Asp Leu Val Lys 
            980                 985                 990         


Val Arg Glu Val Asn Asp Tyr His  His Ala Phe Asp Ala  Tyr Ile Thr 
        995                 1000                 1005             


Val Phe  Val Gly Asn Phe Leu  Tyr Arg Arg Tyr Pro  Lys Leu Arg 
    1010                 1015                 1020             


Ser Tyr  Phe Val Tyr Gly Asp  Phe Lys Lys Leu Asn  Lys Thr Asp 
    1025                 1030                 1035             


Asp Asp  Ile Arg Lys Phe Asn  Phe Leu Asn Leu Phe  Asn Lys Asp 
    1040                 1045                 1050             


Lys Val  Glu Asp Asn Glu Thr  Asp Glu Ile Leu Trp  Ser Lys Ala 
    1055                 1060                 1065             


Glu Ser  Ile Arg Asp Ile Lys  Asn Ile Tyr Asn Tyr  Lys Phe Met 
    1070                 1075                 1080             


Leu Val  Ser Arg Glu Val Tyr  Thr Arg His Gly Ala  Met Phe Asn 
    1085                 1090                 1095             


Gln Thr  Ile Tyr Pro Ala Ser  Lys Asn Asp Ser Lys  Lys Leu Ile 
    1100                 1105                 1110             


Pro Ile  Lys Lys Gly Lys Asp  Thr Ala Ile Tyr Gly  Gly Tyr Ser 
    1115                 1120                 1125             


Gly Asn  Thr Asp Ala Tyr Met  Ala Ile Val Ala Val  Glu Asp Lys 
    1130                 1135                 1140             


Lys Gly  Glu Lys Phe Lys Val  Val Gly Val Pro Thr  Arg Ala Val 
    1145                 1150                 1155             


Ser Arg  Leu Glu Gln Leu Ala  Ala Asp Asp His Asp  Gln Tyr Leu 
    1160                 1165                 1170             


Thr Ala  Leu His Asp Val Leu  Lys Pro Asn Phe Thr  Lys Ile Lys 
    1175                 1180                 1185             


Thr Asn  Arg Lys Thr Lys Ala  Lys Glu Glu Val Thr  Thr Pro Phe 
    1190                 1195                 1200             


Arg Ile  Leu Val Gly Lys Val  Gly Tyr Arg Gln Leu  Ile Gln Asp 
    1205                 1210                 1215             


Gly Gln  Val Lys Met Met Leu  Gly Ser Ser Ala Tyr  Lys Tyr Asn 
    1220                 1225                 1230             


Ala Lys  Gln Leu Val Leu Ser  Glu Lys Ser Leu Lys  Val Ile Lys 
    1235                 1240                 1245             


Asn Asn  Lys Lys Tyr Asn Pro  Asp Asp Glu Asn Gln  Asp Leu Ile 
    1250                 1255                 1260             


Asp Val  Tyr Asp Glu Ile Leu  Gly Val Val Asn Arg  Ala Phe Asn 
    1265                 1270                 1275             


Leu Tyr  Asp Thr Asn Gly Phe  Arg Lys Lys Leu Asn  Asp Asn Arg 
    1280                 1285                 1290             


Val Lys  Phe Val Glu Leu Pro  Ile Asn Asn Ile Phe  Asp Gly Thr 
    1295                 1300                 1305             


Lys Leu  Val Gln Met Gly Lys  Arg Ala Val Leu Thr  Gln Ile Leu 
    1310                 1315                 1320             


Asn Gly  Leu His Ala Asn Ala  Ala Leu Gly Asp Leu  Lys Ala Leu 
    1325                 1330                 1335             


Gly Ile  Ser Thr Pro Phe Gly  Lys Met Gln Ser Lys  Ser Gly Ile 
    1340                 1345                 1350             


Pro Leu  Ser Met Asp Ala Ser  Leu Val Tyr Gln Ser  Pro Thr Gly 
    1355                 1360                 1365             


Leu Phe  Glu Arg Lys Leu Asn  Leu Ser Asp Leu 
    1370                 1375                 


<210>  1088
<211>  1379
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1088

Met Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ala Asp Glu Met Ala Lys Val Asp Glu 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Arg Trp Asp Asp Asp 
            100                 105                 110         


Asn Lys Lys Leu Gly Arg Tyr Pro Ile Phe Gly Asn Lys Ala Asp Val 
        115                 120                 125             


Val Lys Tyr His Gln Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His 
    130                 135                 140                 


Leu Ala Asp Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala 
145                 150                 155                 160 


Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu 
                165                 170                 175     


Leu Asn Ala Glu Asn Thr Asp Val Gln Lys Ile Phe Ser Asp Phe Val 
            180                 185                 190         


Arg Val Tyr Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr 
        195                 200                 205             


Val Asp Ala Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg 
    210                 215                 220                 


Leu Glu Asn Leu Ile Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu 
225                 230                 235                 240 


Phe Gly Asn Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys 
                245                 250                 255     


Met Asn Phe Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp 
            260                 265                 270         


Ser Tyr Asn Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp 
        275                 280                 285             


Tyr Ala Asp Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu 
    290                 295                 300                 


Leu Ser Gly Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu 
305                 310                 315                 320 


Ser Ala Ser Met Ile Lys Arg Tyr Ala Glu His His Glu Asp Leu Glu 
                325                 330                 335     


Lys Leu Lys Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp 
            340                 345                 350         


Ile Phe Lys Asp Glu Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn 
        355                 360                 365             


Gly Val Lys Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Asn Thr Leu Ser 
    370                 375                 380                 


Lys Ile Ala Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp 
385                 390                 395                 400 


Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln 
                405                 410                 415     


Ile His Leu Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr 
            420                 425                 430         


Tyr Pro Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr 
        435                 440                 445             


Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg 
    450                 455                 460                 


Phe Ser Trp Ala Glu Tyr His Ser Asp Glu Lys Ile Thr Pro Trp Asn 
465                 470                 475                 480 


Phe Asp Lys Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr 
                485                 490                 495     


Arg Met Thr Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro 
            500                 505                 510         


Lys His Ser His Val Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr 
        515                 520                 525             


Lys Ile Lys Tyr Val Asn Glu Gln Gly Lys Asp Ser Phe Phe Asp Ser 
    530                 535                 540                 


Asn Met Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys 
545                 550                 555                 560 


Val Thr Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu 
                565                 570                 575     


Tyr Arg Ile Lys Asp Leu Ile Gly Leu Asp Lys Glu Asn Lys Ser Phe 
            580                 585                 590         


Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Leu Asp Lys 
        595                 600                 605             


Ala Phe Leu Asp Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile 
    610                 615                 620                 


Lys Thr Leu Thr Leu Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu 
625                 630                 635                 640 


Gln Lys Tyr Ser Asp Ile Phe Thr Ala Asp Gln Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn 
            660                 665                 670         


Gly Ile Arg Asn Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile 
        675                 680                 685             


Asp Asp Gly Ser Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp 
    690                 695                 700                 


Thr Leu Pro Phe Lys Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp 
705                 710                 715                 720 


Val Asp Asp Ile Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Lys Val Met Gly Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu 
    770                 775                 780                 


Lys Lys Leu Gln Asn Ser Leu Lys Glu Leu Gly Ser Asn Ile Leu Asn 
785                 790                 795                 800 


Glu Glu Lys Pro Ser Tyr Ile Glu Asp Lys Val Glu Asn Ser His Leu 
                805                 810                 815     


Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met 
            820                 825                 830         


Tyr Thr Gly Asp Glu Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile 
        835                 840                 845             


Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp Ser Ile Asp Asn 
    850                 855                 860                 


Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val 
865                 870                 875                 880 


Pro Ser Leu Asp Ile Val Arg Ala Arg Lys Ala Glu Trp Val Arg Leu 
                885                 890                 895     


Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys 
            900                 905                 910         


Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala Gly Phe Ile Lys 
        915                 920                 925             


Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile 
    930                 935                 940                 


Leu Asp Ala Arg Phe Asn Thr Glu Ser Asp Glu Asn Asp Lys Val Ile 
945                 950                 955                 960 


Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe 
                965                 970                 975     


Arg Lys Asp Phe Glu Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His 
            980                 985                 990         


His Ala His Asp Ala Tyr Leu Asn  Ala Val Val Gly Thr  Ala Leu Leu 
        995                 1000                 1005             


Lys Lys  Tyr Pro Lys Leu Ala  Ser Glu Phe Val Tyr  Gly Glu Tyr 
    1010                 1015                 1020             


Lys Lys  Tyr Asp Val His Lys  Leu Ile Ala Lys Ser  Ser Asp Asp 
    1025                 1030                 1035             


His Ser  Glu Met Gly Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser 
    1040                 1045                 1050             


Asn Leu  Met Asn Phe Phe Lys  Arg Val Ile Arg Tyr  Ser Asn Gly 
    1055                 1060                 1065             


Lys Val  Ile Val Arg Pro Val  Val Glu Tyr Ser Lys  Asp Thr Glu 
    1070                 1075                 1080             


Asp Ile  Ala Trp Asp Lys Lys  Ser Asn Phe Arg Thr  Ile Cys Lys 
    1085                 1090                 1095             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Val Glu Thr 
    1100                 1105                 1110             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Pro  Lys Gly Asp 
    1115                 1120                 1125             


Ser Asp  Lys Leu Ile Pro Arg  Lys Thr Lys Lys Ala  Tyr Trp Asp 
    1130                 1135                 1140             


Thr Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser 
    1145                 1150                 1155             


Val Phe  Val Val Ala Asp Val  Glu Lys Gly Lys Ala  Lys Lys Leu 
    1160                 1165                 1170             


Lys Thr  Val Lys Glu Leu Val  Gly Ile Ser Ile Met  Glu Arg Ser 
    1175                 1180                 1185             


Phe Phe  Glu Glu Asn Pro Val  Glu Phe Leu Glu Asn  Lys Gly Tyr 
    1190                 1195                 1200             


His Asn  Ile Arg Glu Asp Lys  Leu Ile Lys Leu Pro  Lys Tyr Ser 
    1205                 1210                 1215             


Leu Phe  Glu Phe Glu Gly Gly  Lys Arg Arg Leu Leu  Ala Ser Ala 
    1220                 1225                 1230             


Ser Glu  Leu Gln Lys Gly Asn  Glu Met Val Ile Pro  Gly His Leu 
    1235                 1240                 1245             


Val Lys  Leu Leu Tyr His Ala  Gln Arg Ile Asn Ser  Phe Asn Ser 
    1250                 1255                 1260             


Thr Lys  Tyr Leu Asp Tyr Val  Ser Ala His Lys Lys  Glu Phe Glu 
    1265                 1270                 1275             


Lys Val  Leu Ser Cys Val Glu  Asp Phe Ala Asn Leu  Tyr Val Asp 
    1280                 1285                 1290             


Val Glu  Lys Asn Leu Ser Lys  Ile Arg Ala Val Ala  Asp Ser Met 
    1295                 1300                 1305             


Asp Asn  Phe Ser Ile Glu Glu  Ile Ser Asn Ser Phe  Ile Asn Leu 
    1310                 1315                 1320             


Leu Thr  Leu Thr Ala Leu Gly  Ala Pro Ala Asp Phe  Asn Phe Leu 
    1325                 1330                 1335             


Gly Glu  Lys Ile Pro Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Cys 
    1340                 1345                 1350             


Leu Asn  Ala Thr Leu Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu 
    1355                 1360                 1365             


Thr Arg  Ile Asp Leu Ser Lys  Ile Gly Glu Glu 
    1370                 1375                 


<210>  1089
<211>  1380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1089

Met Gly Lys Tyr Asn Leu Gly Leu Asp Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Val Asp Glu Asn Asn Gln Ile Val Lys Lys Gly Gly Lys 
            20                  25                  30          


Ala Leu Trp Gly Val Arg Met Phe Asp Glu Ala Gln Ser Ala Lys Glu 
        35                  40                  45              


Thr Arg Ile Tyr Arg Asn Ser Arg Arg Arg Ile Val Arg Arg Asn Gln 
    50                  55                  60                  


Arg Leu Leu Leu Leu Gln Lys Glu Phe Tyr Asp Glu Ile Ile Lys Val 
65                  70                  75                  80  


Asp Gln Thr Phe Phe Gln Arg Leu Lys Asp Ser Phe Phe Lys Lys Glu 
                85                  90                  95      


Asp Lys Leu Asn Lys Asn Asn Tyr Thr Phe Phe Asp Asp Asp Ile Thr 
            100                 105                 110         


Asp Arg Glu Tyr Phe Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Ser 
        115                 120                 125             


His Leu Leu Tyr Asn Asp Glu Lys Ile Asp Ile Arg Met Leu Tyr Leu 
    130                 135                 140                 


Ala Leu His His Ile Val Lys Tyr Arg Gly His Phe Leu Asp Ser Gly 
145                 150                 155                 160 


Glu Val Asn Glu Ser Asn Phe Ser Lys Ile Lys Asp Val Leu Asp Thr 
                165                 170                 175     


Phe Asn Asn Lys Met Ser Glu Met Ser Leu Glu Phe Glu Asp Asn Glu 
            180                 185                 190         


Asp Tyr Phe Cys Lys Ile Glu Phe Asp Glu Asn Phe Glu Asn Lys Leu 
        195                 200                 205             


Lys Asp Ile Met Leu Ser Gln Leu Ser Lys Lys Glu Lys Arg Gln Glu 
    210                 215                 220                 


Leu Ile Asp Leu Phe Lys Val Asp Lys Lys Ser Leu Val Asn Glu Leu 
225                 230                 235                 240 


Leu Ile Glu Leu Leu Val Asn Lys Lys Val Lys Leu Asn Ser Leu Ser 
                245                 250                 255     


Ile Thr Asn Gly Lys Lys Tyr Glu Val Cys Glu Ile Asp Phe Glu Lys 
            260                 265                 270         


Glu Glu Leu Phe Glu Lys Leu Glu Asp Ala Lys Lys Ile Ile Thr Glu 
        275                 280                 285             


Leu Phe Asp Ile Leu Asp Glu Ile Val Asn Ile Lys Lys Ile Val Asp 
    290                 295                 300                 


Tyr Tyr Tyr Val Leu Lys Ile Ile Gly Asp Asn Glu Tyr Leu Ser Glu 
305                 310                 315                 320 


Ala Met Val Lys Lys Tyr Asp Lys His Asp Lys Asp Leu Arg Glu Leu 
                325                 330                 335     


Lys Ser Phe Ile Lys Thr Tyr Cys Pro Asn Lys Tyr Tyr Glu Cys Phe 
            340                 345                 350         


Arg Lys Thr Ser Asn Pro Asn Leu Cys Asn Tyr Pro Lys Tyr Val Gly 
        355                 360                 365             


Ile Asn Ser Val Asn Lys Lys Ile Glu Arg Phe Ala His Cys Lys Lys 
    370                 375                 380                 


Glu Glu Phe Tyr Lys Tyr Leu Lys Ser Leu Phe Asn Thr Ile Lys Asp 
385                 390                 395                 400 


Glu Asn Ala Lys Leu Lys Ile Asp Leu Phe Gln Lys Asp Met Glu Asn 
                405                 410                 415     


Asp Asp Phe Leu Asn Arg Gln Asn Ser Asn His Asn Ser Ser Ile Pro 
            420                 425                 430         


Met Gln Leu Asn Phe Cys Glu Leu Lys Lys Ile Leu Glu Asn Gln Ser 
        435                 440                 445             


Lys Tyr Tyr Pro Phe Leu Met Leu Lys Asn Ser Asp Gly Tyr Ser Thr 
    450                 455                 460                 


Ser Asp Arg Ile Ile Ala Ile Phe Lys Tyr Lys Ile Pro Tyr Tyr Val 
465                 470                 475                 480 


Gly Pro Leu Asn Lys Asn Ser Lys Tyr Ser Trp Leu Val Lys Lys Ser 
                485                 490                 495     


Asn Glu Asn Ile Thr Pro Trp Asn Tyr Glu Lys Val Ile Asp Leu Asp 
            500                 505                 510         


Ala Ser Ala Lys Glu Phe Ile Gln Arg Met Gln Asn Lys Cys Thr Tyr 
        515                 520                 525             


Leu Lys Gly Glu Asn Asp Tyr Cys Leu Pro Lys Lys Ser Ile Leu Phe 
    530                 535                 540                 


Ser Glu Tyr Asn Cys Leu Gln Tyr Leu Asn Arg Leu Ile Ile Asn Gly 
545                 550                 555                 560 


Ala Leu Ile Glu Asn Lys Leu Lys Asn Glu Ile Tyr Glu Asn Ile Phe 
                565                 570                 575     


Leu Cys Tyr Lys Lys Pro Thr Lys Lys Ser Ile Val Glu Tyr Leu Lys 
            580                 585                 590         


Thr Asn Tyr Asn Tyr Asp Glu Thr Thr Leu Lys Ser Asn Ile Leu Glu 
        595                 600                 605             


Val Asn Cys Asp Met Ser Ser Tyr Ile Gln Phe Lys Glu Ile Tyr Gly 
    610                 615                 620                 


Glu Asp Phe Glu Gln Asn Leu Asn Ile Val Glu Asp Ile Ile Lys Asp 
625                 630                 635                 640 


Ile Thr Ile Phe Glu Asp Lys Lys Ile Leu Glu Lys Arg Leu Arg Glu 
                645                 650                 655     


Ile Tyr His Leu Ser Glu Asp Lys Val Gln Lys Ile Lys Gly Leu Asn 
            660                 665                 670         


Tyr Lys Gly Tyr Ala Thr Leu Ser Lys His Phe Leu Asn Asn Phe Tyr 
        675                 680                 685             


Ser Val Asn Glu Glu Thr Gly Glu Ile Tyr Gly Pro Ile Ile Gln Ile 
    690                 695                 700                 


Met Arg Asp Thr Asn Tyr Asn Leu Gln Gln Val Ile Tyr Asn Asp Arg 
705                 710                 715                 720 


Tyr Asn Phe Met Lys Ala Ile Asp Lys Tyr Asn Lys Lys Asn Leu Asp 
                725                 730                 735     


Thr Glu Lys Lys Leu Asn Val Ser Asp Phe Ile Asp Glu Tyr Ile Tyr 
            740                 745                 750         


Val Ser Pro Ile Met Lys Arg Ser Leu Ile Gln Ser Tyr Asn Ile Ile 
        755                 760                 765             


Gln Glu Leu Glu Lys Ile Leu Gly Glu Ser Ile Ser Lys Tyr Tyr Ile 
    770                 775                 780                 


Glu Cys Ala Arg Val Arg Ser Asn Ser Lys Asp Val Ile Asn Ser Arg 
785                 790                 795                 800 


Tyr Arg Gln Leu Ser Glu Leu Tyr Lys Asp Cys Glu His Gln Val Ser 
                805                 810                 815     


Asp Ile Ser Lys Asn Tyr Ile Asn Ile Asn Lys Leu Lys Lys Gln Leu 
            820                 825                 830         


Glu Asp Asn Lys Asn Glu Leu Arg Ala Asp Lys Leu Tyr Leu Tyr Phe 
        835                 840                 845             


Thr Gln Phe Gly Lys Cys Met Tyr Thr Leu Lys Asp Ile Asp Leu Asp 
    850                 855                 860                 


Asp Val Phe Leu Gly Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser 
865                 870                 875                 880 


Leu Ile Lys Asp Asp Ser Leu Asn Asn Arg Val Leu Ala Ser Lys Glu 
                885                 890                 895     


Lys Asn Gly Lys Lys Asp Asn Gln Phe Ile Phe Asn Ile Pro Glu Phe 
            900                 905                 910         


Leu Pro Lys Asn Ala Glu Leu Phe Tyr Lys Lys Leu Leu Glu Cys Lys 
        915                 920                 925             


Leu Ile Thr Lys Glu Lys Tyr Arg Arg Leu Thr Met Lys Lys Ile Asp 
    930                 935                 940                 


Asp Asp Glu Leu Asp Ile Phe Val Asn Arg Gln Leu Val Ala Thr Asn 
945                 950                 955                 960 


Gln Ser Val Lys Gly Leu Val Asp Val Leu Lys Ile Tyr Lys Gly Val 
                965                 970                 975     


Lys Pro Thr Asn Ile Val Tyr Ser Lys Ala Val Asn Ile Ser Asp Phe 
            980                 985                 990         


Arg Asn Asn Tyr Asn Leu Tyr Lys  Ser Arg Thr Ala Asn  Asn Tyr His 
        995                 1000                 1005             


His Ala  His Asp Ala Tyr Leu  Asn Val Val Ile Gly  Lys Ala Leu 
    1010                 1015                 1020             


Asp Val  Tyr Tyr Gln Cys Asn  Tyr Phe Gln Asn Tyr  Lys Asp Val 
    1025                 1030                 1035             


Ile Lys  Leu Lys Asp Arg Asn  Ile Thr Ile Asn Pro  Glu Ile Ile 
    1040                 1045                 1050             


Leu Ser  Lys Asn Arg Tyr Val  Asn Arg Asn Gly Glu  Lys Lys Val 
    1055                 1060                 1065             


Ile Trp  Asp Leu Asp Asn Thr  Leu Lys Leu Ile Lys  Asn Asn Leu 
    1070                 1075                 1080             


Tyr Asn  Arg Phe Asp Ile Thr  Glu Thr Ile Met Thr  Arg Lys Ser 
    1085                 1090                 1095             


Asn Lys  Met Phe Ser Lys Ser  Ser Ile Ser Pro Ala  Lys Asn Ser 
    1100                 1105                 1110             


Lys Asn  Leu Ile Pro Ile Lys  Thr Ser Asp Pro Arg  Cys Asn Val 
    1115                 1120                 1125             


Leu Lys  Tyr Gly Gly Ile Lys  Ser Asn Ser Tyr Asn  Phe Tyr Cys 
    1130                 1135                 1140             


Ile Val  Glu Leu Tyr Asp Lys  Lys Gly Lys Lys Ser  Tyr Ile Leu 
    1145                 1150                 1155             


Glu Ala  Ile Pro Lys Thr His  Ser Asp Lys Leu Glu  Glu Tyr Ile 
    1160                 1165                 1170             


Lys Asn  Ser Ile Asp Glu Lys  Asn Val Ser Asn Phe  Lys Ile Ile 
    1175                 1180                 1185             


Asn Lys  Asn Ile Lys Ser Asn  Ile Ile Ile Glu Tyr  Lys Lys Leu 
    1190                 1195                 1200             


Arg Tyr  Val Ile Thr Gly Lys  Ser Gly Lys Tyr Tyr  Leu Ile Lys 
    1205                 1210                 1215             


Asn Phe  Lys Asp Arg Asn Phe  Ser Tyr Asn Ala Ile  Lys Thr Ile 
    1220                 1225                 1230             


Lys Tyr  Ile Glu Lys Tyr Asn  Tyr Gln Lys Lys Tyr  Glu Ile Pro 
    1235                 1240                 1245             


Phe Glu  Glu Thr Lys Glu Lys  Ile Ile Leu Ala Pro  Lys Lys Lys 
    1250                 1255                 1260             


Asp Thr  Lys Glu Ile Ser Leu  Thr Leu Asp Asn Cys  Gln Ala Leu 
    1265                 1270                 1275             


Leu Asp  Glu Ile Ile Ser Leu  Phe Lys Lys Asp Ile  Tyr Asn Tyr 
    1280                 1285                 1290             


Asp Ile  Ile Tyr Lys Ile Thr  Ser Lys Leu Glu Glu  Asn Lys Gln 
    1295                 1300                 1305             


Asn Leu  Thr Leu Thr Asn Leu  Ile Lys Val Leu Thr  Glu Ile Leu 
    1310                 1315                 1320             


Ile Leu  Leu Lys Thr Asn Glu  Arg Gly Thr Ala Asp  Leu Thr Leu 
    1325                 1330                 1335             


Ile Asp  Met Ser Lys Asp Thr  Gly Thr Leu Cys Ile  Ser Lys Thr 
    1340                 1345                 1350             


Leu Lys  Pro Gly Met Lys Phe  Ile Ser Glu Ser Pro  Thr Gly Tyr 
    1355                 1360                 1365             


Tyr Arg  Lys Ile Ile Phe Glu  Val Pro Asn Gly Ile  
    1370                 1375                 1380 


<210>  1090
<211>  1380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1090

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Asn Asn Glu Ala Ser Ser Ile Val 
            20                  25                  30          


Arg Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Lys Ser Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ala Ile Thr Thr Asn Ala Asp Arg Gln Leu Arg 
    50                  55                  60                  


His Gly Ala Arg Ile Asn Leu Gln Arg Tyr Lys Leu Arg Arg Gln Asn 
65                  70                  75                  80  


Leu His Asp Cys Leu Gln Lys Gln Gly Trp Leu Gly Thr Glu Ala Met 
                85                  90                  95      


Tyr Glu Glu Gly Lys Ala Ser Thr Phe Glu Thr Tyr Lys Leu Arg Ala 
            100                 105                 110         


Lys Ala Ala Glu Glu Glu Ile Ser Leu His Glu Phe Ala Arg Val Leu 
        115                 120                 125             


Phe Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Asn Arg Lys Ala Asn 
    130                 135                 140                 


Asn Lys Glu Asp Gly Gln Leu Phe Asp Gly Met Thr Ile Ala Lys Lys 
145                 150                 155                 160 


Leu Tyr Glu Glu His Leu Thr Pro Ala Glu Tyr Ser Leu Gln Leu Leu 
                165                 170                 175     


Asn Lys Gly Lys Lys Phe Thr Gln Gly Tyr Tyr Arg Ser Asp Leu Asn 
            180                 185                 190         


Ala Glu Leu Glu Arg Ile Trp Asp Glu Gln Lys Lys Tyr Tyr Pro Glu 
        195                 200                 205             


Ile Leu Thr Asp Glu Phe Lys Gln Gln Leu Glu Gly Lys Thr Lys Thr 
    210                 215                 220                 


Asn Thr Ser Lys Ile Phe Leu Ala Lys Tyr Gly Ile Tyr Ser Ala Asp 
225                 230                 235                 240 


Leu Lys Gly Leu Asp Arg Lys Phe Gln Pro Leu Lys Trp Arg Val Glu 
                245                 250                 255     


Ala Leu Gln Gln Gln Val Asp Lys Glu Val Leu Ala Phe Val Ile Ser 
            260                 265                 270         


Asp Leu Lys Gly Gln Ile Ala Asn Thr Ser Gly Leu Leu Gly Ala Ile 
        275                 280                 285             


Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln 
    290                 295                 300                 


Tyr Leu Trp Ala Ser Leu Glu Glu Asn Pro His Ile Ser Ile Lys Asn 
305                 310                 315                 320 


Lys Pro Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Lys Ile Trp 
                325                 330                 335     


Glu Thr Gln Ala Ala Phe His Lys Gln Leu Thr Pro Glu Leu Lys Gln 
            340                 345                 350         


Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
        355                 360                 365             


Lys Ser Leu Ile Ser Val Cys Glu Leu Glu Gln Arg Lys Val Lys Ala 
    370                 375                 380                 


Thr Ile Asp Gly Lys Glu Lys Glu Ile Thr Ile Gly Pro Lys Val Ala 
385                 390                 395                 400 


Pro Lys Ser Ser Pro Val Phe Gln Glu Phe Arg Ile Trp Gln Asn Leu 
                405                 410                 415     


Asn Asn Val Leu Leu Ile Asp Asn Asp Thr Asn Glu Lys Arg Pro Leu 
            420                 425                 430         


Asp Glu Val Glu Arg Asn Leu Leu Tyr Lys Glu Leu Ser Ile Lys Ala 
        435                 440                 445             


Lys Leu Ser Lys Thr Glu Ala Leu Lys Ile Leu Asn Lys Lys Gly Lys 
    450                 455                 460                 


Gln Trp Asp Leu Asn Tyr Arg Glu Leu Glu Gly Asn Arg Thr Gln Ala 
465                 470                 475                 480 


Ile Leu Phe Asp Cys Tyr Asn Arg Ile Ile Thr Leu Thr Gly His Glu 
                485                 490                 495     


Glu Cys Asp Phe Lys Lys Ile Lys Ala Ser Glu Ile Arg His Tyr Val 
            500                 505                 510         


Ser Thr Ile Phe Lys Asn Leu Gly Phe Ser Thr Glu Ile Leu Asp Phe 
        515                 520                 525             


Asp Pro Ser Leu Lys Lys His Glu Leu Glu Lys Gln Pro Met Tyr Gln 
    530                 535                 540                 


Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asn Ser Arg Thr Gly 
545                 550                 555                 560 


Asn Glu Ser Leu Leu Arg Lys Leu Glu Thr Thr Phe Gly Phe Pro Glu 
                565                 570                 575     


Glu Tyr Ala Thr Val Leu Cys Asp Val Val Phe Glu Glu Asp Tyr Gly 
            580                 585                 590         


Asn Leu Ser Val Lys Ala Met Arg Glu Ile Leu Pro Tyr Leu Gln Ala 
        595                 600                 605             


Gly Asn Asp Tyr Ser Gln Ala Cys Ala Tyr Ala Gly Tyr Asn His Ser 
    610                 615                 620                 


Arg His Ser Leu Thr Lys Glu Glu Leu Asp Gln Lys Val Tyr Lys Glu 
625                 630                 635                 640 


Arg Leu Glu Leu Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
                645                 650                 655     


Lys Ile Leu Asn Gln Met Ile Asn Val Ile Asn Ala Ile Ile Asp Glu 
            660                 665                 670         


Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Met Ala Arg Glu Leu Lys 
        675                 680                 685             


Ser Ser Ala Ala Asp Arg Lys Lys Thr Thr His Ala Ile Ser Gln Gly 
    690                 695                 700                 


Asn Ala Glu Asn Gln Arg Ile Arg Glu Ile Leu Glu Lys Glu Phe Ser 
705                 710                 715                 720 


Leu Ser Tyr Ile Ser Arg Asn Asp Ile Ile Lys Tyr Lys Leu Tyr Glu 
                725                 730                 735     


Glu Leu Glu Pro Asn Tyr Tyr Lys Thr Leu Tyr Ser Asp Thr Tyr Ile 
            740                 745                 750         


Thr Lys Asp Lys Leu Phe Ser Lys Asp Phe Asp Ile Glu His Ile Ile 
        755                 760                 765             


Pro Lys Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
    770                 775                 780                 


Ala Arg Asn Ile Asn Leu Glu Lys Ser Asn Lys Thr Ala Phe Asp Phe 
785                 790                 795                 800 


Ile Lys Glu Lys Tyr Gly Glu Asp Gly Ala Glu Ala Tyr Lys Lys Lys 
                805                 810                 815     


Leu Asp Met Leu Leu Glu Asn Asp Ala Ile Ser Arg Pro Lys Tyr Asn 
            820                 825                 830         


Asn Leu Leu Arg Ala Glu Ala Asp Ile Pro Ser Asp Phe Ile Asn Arg 
        835                 840                 845             


Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala Cys Glu Ile Leu 
    850                 855                 860                 


Gly Glu Leu Val Lys Thr Val Thr Pro Thr Thr Gly Lys Ile Thr Asn 
865                 870                 875                 880 


Arg Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn 
                885                 890                 895     


Phe Glu Lys Tyr Glu Lys Leu Gly Leu Thr Glu Ile Val Glu Asp Arg 
            900                 905                 910         


Asp Gly Arg Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp 
        915                 920                 925             


His Arg His His Ala Met Asp Ala Leu Ala Ile Ala Phe Thr Lys Pro 
    930                 935                 940                 


Ser Phe Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Ser Asn Lys Gly 
945                 950                 955                 960 


Asp Ser Ile Tyr Ala Ile Glu Asn Lys Glu Leu His Tyr Glu Glu Gly 
                965                 970                 975     


Lys Leu Arg Phe Asn Ala Pro Ile Pro Val Asn Glu Phe Arg Ala Glu 
            980                 985                 990         


Ala Lys Arg His Leu Ser Ala Ile  Leu Val Ser Ile Lys  Ala Lys Asn 
        995                 1000                 1005             


Lys Val  Met Thr Gln Asn Val  Asn Lys Ile Lys Thr  Lys His Gly 
    1010                 1015                 1020             


Ile Ile  Lys Lys Ile Gln Leu  Thr Pro Arg Gly Pro  Leu His Asn 
    1025                 1030                 1035             


Glu Thr  Ile Tyr Gly Thr Lys  Met Arg Pro Ile Ile  Lys Met Val 
    1040                 1045                 1050             


Lys Val  Gly Ala Ala Leu Asp  Glu Ala Thr Ile Asn  Lys Val Ser 
    1055                 1060                 1065             


Ser Pro  Ala Ile Arg Glu Ala  Leu Leu Lys Arg Leu  Asn Glu Tyr 
    1070                 1075                 1080             


Ser Gly  Asn Ala Lys Lys Ala  Phe Thr Gly Lys Asn  Thr Leu Glu 
    1085                 1090                 1095             


Lys Asn  Pro Ile Tyr Leu Asn  Ala Gly Arg Thr Lys  Thr Val Pro 
    1100                 1105                 1110             


Ser Leu  Val Lys Thr Val Glu  Trp Glu Ser Phe His  Pro Thr Arg 
    1115                 1120                 1125             


Lys Leu  Ile Asp Lys Asp Leu  Asn Val Asp Lys Val  Val Asp Lys 
    1130                 1135                 1140             


Gly Ile  Arg Glu Ile Leu Lys  Ala Arg Leu Glu Glu  Phe Asn Gly 
    1145                 1150                 1155             


Asp Ala  Lys Lys Ala Phe Ser  Asn Leu Glu Glu Asn  Pro Ile Tyr 
    1160                 1165                 1170             


Leu Asp  Glu Ala Lys Lys Ile  Ala Leu Lys Arg Val  Ser Ile Glu 
    1175                 1180                 1185             


Gly Val  Leu Ser Ala Ile Pro  Leu His Thr Leu Lys  Asn Gln Ala 
    1190                 1195                 1200             


Gly Lys  Pro Ile Thr Gly Lys  Asp Gly Lys Pro Val  Leu Gly Asn 
    1205                 1210                 1215             


Tyr Val  Gln Thr Ser Asn Asn  His His Ile Ala Phe  Tyr Tyr Asp 
    1220                 1225                 1230             


Glu Asp  Gly Asn Leu Gln Asp  Asn Ala Val Ser Phe  Phe Glu Ala 
    1235                 1240                 1245             


Ala Glu  Arg Lys Ser Gln Gly  Ile Pro Val Ile Asp  Lys Asp Tyr 
    1250                 1255                 1260             


Asn Arg  Asp Lys Gly Trp Arg  Phe Leu Phe Thr Met  Lys Gln Asn 
    1265                 1270                 1275             


Glu Tyr  Phe Val Phe Pro Asn  Glu Ala Thr Gly Phe  Ile Pro Ser 
    1280                 1285                 1290             


Glu Val  Asp Leu Thr Asp Glu  Ala Asn Tyr Gly Ile  Ile Ser Pro 
    1295                 1300                 1305             


Asn Leu  Tyr Arg Val Gln Lys  Val Ser Arg Ile Asp  Lys Gly Thr 
    1310                 1315                 1320             


Ser Ala  Ser Arg Asp Tyr Trp  Phe Arg His His Leu  Glu Thr Ile 
    1325                 1330                 1335             


Leu Asn  Asp Asp Ala Lys Leu  Lys Asn Leu Ala Phe  Lys Arg Ile 
    1340                 1345                 1350             


Arg Gly  Leu Leu Glu Leu Lys  Asp Ile Ile Lys Val  Arg Ile Asn 
    1355                 1360                 1365             


Ser Thr  Gly Lys Ile Val Ala  Val Gly Glu Tyr Asp  
    1370                 1375                 1380 


<210>  1091
<211>  1380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1091

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Thr Glu Ser Glu Lys Ser Ser Ile Ile 
            20                  25                  30          


Arg Leu Gly Val Arg Val Asn Pro Leu Thr Ile Asp Glu Lys Gly Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ala Ile Thr Thr Asn Ser Asp Arg Gln Gln Arg 
    50                  55                  60                  


His Ser Ala Arg Ile Asn Leu Gln Arg Tyr Lys Leu Arg Arg Gln Asn 
65                  70                  75                  80  


Leu Cys Asp Cys Leu Gln Ile Gly Gly Leu Leu Gly Ser Glu Ser Met 
                85                  90                  95      


Tyr Glu Glu Gly Lys Glu Ser Thr Phe Glu Thr Tyr Lys Leu Arg Ala 
            100                 105                 110         


Lys Ala Ala Thr Glu Lys Val Ala Leu His Glu Phe Ala Arg Ile Leu 
        115                 120                 125             


Phe Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Asn Arg Lys Ala Asn 
    130                 135                 140                 


Ser Lys Glu Asp Gly Gln Ala Phe Asp Gly Met Thr Ile Ala Lys Lys 
145                 150                 155                 160 


Leu Tyr Glu Glu Asn Leu Thr Pro Ala Glu Tyr Ser Leu Gln Leu Leu 
                165                 170                 175     


Asn Lys Gly Lys Lys Phe Ser Pro Ser Tyr Tyr Arg Ser Asp Leu Glu 
            180                 185                 190         


Ser Glu Leu Asn Arg Ile Trp Glu Glu Gln Lys Lys Tyr Tyr Pro Glu 
        195                 200                 205             


Ile Leu Thr Asp Asp Phe Tyr Gln Gln Leu Glu Gly Lys Thr Lys Val 
    210                 215                 220                 


Asn Thr Thr Lys Ile Phe Leu Ala Lys Tyr Gly Ile Tyr Thr Ala Asp 
225                 230                 235                 240 


Leu Lys Gly Leu Asp Lys Lys Met Gln Pro Leu Lys Trp Arg Ile Glu 
                245                 250                 255     


Ala Leu Asn Lys Gln Val Asp Lys Glu Val Leu Thr Phe Val Val Ser 
            260                 265                 270         


Asp Leu Lys Gly Gln Ile Glu Lys Thr Ser Gly Leu Leu Gly Glu Ile 
        275                 280                 285             


Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln 
    290                 295                 300                 


Tyr Val Trp Ala Ser Leu Lys Glu Asn Pro His Val Ser Ile Lys Asn 
305                 310                 315                 320 


Lys Pro Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Lys Ile Trp 
                325                 330                 335     


Glu Thr Gln Ala Ala Phe His Lys Glu Leu Thr Pro Glu Leu Lys Lys 
            340                 345                 350         


Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
        355                 360                 365             


Lys Gly Leu Ile Ser Ile Cys Glu Leu Glu Gln Lys Lys Glu Lys Val 
    370                 375                 380                 


Ile Ile Glu Gly Lys Glu Lys Glu Ile Leu Ile Gly Ser Lys Val Ala 
385                 390                 395                 400 


Pro Lys Ser Ser Pro Met Tyr Gln Glu Phe Arg Ile Trp Gln Asn Leu 
                405                 410                 415     


Asn Asn Val Leu Leu Ile Asp Thr Glu Ile Asn Glu Ser Arg Pro Leu 
            420                 425                 430         


Asp Glu Glu Glu Lys Ala Leu Leu Tyr Asn Glu Leu Ser Val Arg Ala 
        435                 440                 445             


Lys Leu Ser Glu Thr Glu Ala Leu Lys Thr Ile Asn Lys Lys Gly Lys 
    450                 455                 460                 


His Trp Asp Leu Asn Phe Lys Glu Leu Glu Gly Asn Arg Thr Gln Ala 
465                 470                 475                 480 


Val Leu Phe Glu Cys Tyr Asn Arg Ile Ile Thr Gln Thr Gly His Glu 
                485                 490                 495     


Glu Cys Asn Phe Lys Lys Ile Ser Ala Glu Asp Ile Arg His Tyr Val 
            500                 505                 510         


Ala Thr Ile Phe Lys Asn Leu Gly Phe Asn Thr Asp Ile Leu Asp Phe 
        515                 520                 525             


Asn Pro Ser Leu Glu Lys His Glu Leu Glu Lys Gln Pro Met Tyr Gln 
    530                 535                 540                 


Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Lys Ser Arg Thr Gly 
545                 550                 555                 560 


Asn Glu Ser Leu Leu Asn Lys Leu Glu Ala Asn Phe Gly Phe Pro Gln 
                565                 570                 575     


Glu Tyr Ala Ser Val Leu Ala Asp Ala Thr Phe Glu Asp Asp Tyr Gly 
            580                 585                 590         


Asn Ile Ser Val Lys Ala Ile Arg Lys Ile Leu Pro His Leu His Asp 
        595                 600                 605             


Gly Tyr Asp Tyr Ser Glu Ala Cys Glu Arg Ala Gly Tyr Asn His Ser 
    610                 615                 620                 


Val Arg Ser Leu Thr Lys Glu Glu Leu Ala Gln Lys Val Tyr Lys Asp 
625                 630                 635                 640 


Arg Leu Asp Leu Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
                645                 650                 655     


Lys Ile Leu Asn Gln Met Ile Asn Val Ile Asn Ala Ile Ile Val Glu 
            660                 665                 670         


Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Met Ala Arg Glu Leu Lys 
        675                 680                 685             


Ser Ser Ala Ala Lys Arg Glu Glu Arg Thr Lys Ser Ile Asn Gln Asn 
    690                 695                 700                 


Ala Ala Glu Asn Lys Arg Ile Thr Glu Ile Leu Gln Thr Glu Phe Gly 
705                 710                 715                 720 


Leu Ser Tyr Ile Ser Arg Asn Asp Ile Val Lys Tyr Lys Leu Tyr Gln 
                725                 730                 735     


Glu Leu Glu Ala Asn Gly Phe Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
            740                 745                 750         


Pro Lys Glu Lys Leu Phe Ser Lys Asp Phe Asp Ile Glu His Ile Val 
        755                 760                 765             


Pro Lys Ala Arg Leu Phe Asp Asp Ser Phe Gly Asn Lys Thr Ile Glu 
    770                 775                 780                 


Ala Arg Asp Val Asn Leu Ala Lys Ser Asn Lys Thr Ala Phe Asp Phe 
785                 790                 795                 800 


Val Lys Glu Lys Tyr Gly Glu Gln Gly Ile Glu Glu Tyr Lys Lys Lys 
                805                 810                 815     


Leu Asp Thr Leu Leu Lys Glu Asn Ala Ile Ser Ser Gly Lys Tyr Lys 
            820                 825                 830         


Lys Leu Met Met Ser Glu Ala Asp Ile Pro Ser Asp Phe Ile Asn Arg 
        835                 840                 845             


Asp Leu Arg Asn Thr Gln Tyr Ile Ala Arg Lys Ala Tyr Glu Ile Leu 
    850                 855                 860                 


Gly Glu Leu Val Lys Thr Ile Thr Pro Thr Thr Gly Lys Ile Thr Asp 
865                 870                 875                 880 


Arg Leu Arg Glu Asp Trp Gln Leu Ile Asp Val Met Lys Glu Leu Asn 
                885                 890                 895     


Phe Glu Lys Tyr Asp Arg Leu Gly Leu Thr Glu Val Ile Glu Asp Arg 
            900                 905                 910         


Asp Gly Arg Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp 
        915                 920                 925             


His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr Lys Pro 
    930                 935                 940                 


Ser Tyr Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Ser Asp Lys Ser 
945                 950                 955                 960 


Gly Ser Ile Tyr Ala Ile Glu Thr Lys Glu Leu His Arg Glu Asn Asn 
                965                 970                 975     


Thr Leu Arg Phe Asn Ala Pro Met Pro Leu Asn Glu Phe Arg Lys Glu 
            980                 985                 990         


Ala Lys Arg His Leu Ser Ala Ile  Leu Val Ser Ile Lys  Ala Lys Asn 
        995                 1000                 1005             


Lys Val  Met Thr Gln Asn Val  Asn Lys Ile Lys Thr  Lys Asn Gly 
    1010                 1015                 1020             


Ile Ile  Lys Lys Val Gln Leu  Thr Pro Arg Gly Ala  Leu His Asn 
    1025                 1030                 1035             


Glu Thr  Ile Tyr Gly Thr Lys  Met Arg Pro Ile Ile  Lys Met Val 
    1040                 1045                 1050             


Lys Ile  Gly Ala Ala Leu Asp  Glu Ala Thr Ile Asn  Lys Val Ser 
    1055                 1060                 1065             


Ser Pro  Thr Ile Arg Glu Ala  Leu Leu Lys Arg Leu  Asn Glu Tyr 
    1070                 1075                 1080             


Ser Gly  Asp Ala Lys Lys Ala  Phe Ala Gly Lys Asn  Ala Ile Thr 
    1085                 1090                 1095             


Lys Asn  Pro Ile Tyr Leu Asn  Ala Glu His Thr Lys  Thr Ala Pro 
    1100                 1105                 1110             


Ala Met  Val Lys Ile Val Glu  Trp Glu Met Tyr Tyr  Pro Ile Arg 
    1115                 1120                 1125             


Lys Leu  Ile Thr Pro Asp Leu  Lys Ile Glu Lys Val  Ile Asp Asn 
    1130                 1135                 1140             


Gly Ile  Lys Glu Ile Leu Lys  Lys Arg Leu Lys Glu  Phe Gly Gly 
    1145                 1150                 1155             


Asp Ala  Lys Lys Ala Phe Ser  Asn Leu Asp Glu Cys  Pro Ile Tyr 
    1160                 1165                 1170             


Leu Asp  Glu Glu Lys Asn Ile  Thr Leu Lys Arg Val  Ser Ile Glu 
    1175                 1180                 1185             


Gly Val  Leu Ser Ala Ile Pro  Val His Thr Leu Lys  Asn His Ala 
    1190                 1195                 1200             


Gly Lys  Glu Ile Lys Asp Glu  Asp Gly Lys Ala Val  Leu Gly Asp 
    1205                 1210                 1215             


Tyr Val  Gln Thr Ser Asn Asn  His His Val Ala Ile  Tyr Gln Asp 
    1220                 1225                 1230             


Ala Lys  Gly Asn Leu Gln Asp  Asn Val Ile Ser Phe  Phe Glu Ala 
    1235                 1240                 1245             


Ala Glu  Arg Lys Ser Gln Asn  Val Pro Ile Val Asp  Lys Asn Leu 
    1250                 1255                 1260             


Asn Arg  Asp Lys Gly Trp His  Phe Leu Tyr Thr Met  Lys Gln Asn 
    1265                 1270                 1275             


Glu Tyr  Phe Val Phe Pro Asn  Glu Glu Thr Gly Phe  Val Pro Ser 
    1280                 1285                 1290             


Glu Ile  Asp Leu Met Asp Glu  Asn Asn Tyr Glu Met  Ile Ser Pro 
    1295                 1300                 1305             


Asn Leu  Phe Arg Val Gln Thr  Met Ser Arg Val Asp  Tyr Gly Lys 
    1310                 1315                 1320             


Thr Ile  Ile Arg Glu Tyr Val  Phe Arg Asn His Leu  Glu Thr Ile 
    1325                 1330                 1335             


Leu Asn  Asp Asn Leu Lys Leu  Lys Gly Leu Val Phe  Lys Lys Ile 
    1340                 1345                 1350             


Lys Ser  Leu Lys Ala Leu Glu  Gly Ile Val Lys Val  Arg Val Asn 
    1355                 1360                 1365             


Ala Ile  Gly Lys Ile Val Ala  Val Gly Glu Tyr Asp  
    1370                 1375                 1380 


<210>  1092
<211>  1380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1092

Met Lys Lys Lys Tyr Ala Ile Gly Ile Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Val Val Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Phe Gly Asn Thr Glu Lys Arg Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Thr Leu Leu Phe Asp Glu Gly Asn Thr Ala Glu Asn Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Lys Ile Asp Asp Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Ile Val Glu Asp Lys Gln 
            100                 105                 110         


Gly Ser Lys His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys Glu Tyr 
        115                 120                 125             


His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala Asp 
    130                 135                 140                 


Ser Ser Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Lys Ser 
                165                 170                 175     


Glu Asn Lys Asp Val Gln His Leu Phe Asn Asp Phe Val Glu Met Phe 
            180                 185                 190         


Asp Lys Thr Val Glu Gly Ser Tyr Leu Ser Glu Asn Leu Pro Asn Val 
        195                 200                 205             


Ala Asp Val Leu Val Glu Lys Val Ser Lys Ser Arg His Leu Glu Asn 
    210                 215                 220                 


Ile Leu His Tyr Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Phe Leu Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     


Glu Leu Ala Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         


Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             


Leu Phe Ile Ala Thr Lys Ser Leu Tyr Asp Gly Ile Leu Leu Ala Gly 
    290                 295                 300                 


Ile Leu Ser Thr Thr Asn Ser Thr Thr Lys Ala Pro Leu Ser Ser Ser 
305                 310                 315                 320 


Met Val Asn Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu Lys 
                325                 330                 335     


Asn Phe Ile His Gln Asn Leu Ser Asp Ser Tyr Lys Glu Val Phe Asn 
            340                 345                 350         


Asp Lys Leu Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             


Gln Glu Asn Phe Tyr Arg Phe Ile Lys Lys Ala Ile Glu Lys Ile Asp 
    370                 375                 380                 


Gly Ser Asn Tyr Phe Ile Glu Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Arg Glu Met Gln Ala Ile Ile Arg Arg Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         


Leu Glu Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Glu Phe Ala Trp 
    450                 455                 460                 


Leu Asn Arg Lys Ser Asp Glu Lys Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 


Met Val Asp Lys Glu Thr Ser Ala Glu Asn Phe Ile Thr Arg Met Thr 
                485                 490                 495     


Asn Tyr Asp Gln Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             


Tyr Val Thr Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp Arg Thr Phe Lys Val Tyr Arg Lys Val Thr Lys 
545                 550                 555                 560 


Glu Lys Leu Met Asp Phe Leu Ser Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     


Val Asp Leu Leu Gly Leu Asp Lys Asp Asn Lys Ser Phe Asn Ala Ser 
            580                 585                 590         


Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Val Ser Glu Asp Phe Leu 
        595                 600                 605             


Asp Asn Ser Glu Asn Glu Asp Ile Leu Glu Asn Val Val Leu Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr 
625                 630                 635                 640 


Lys Asp Val Leu Thr Glu Glu Gln Arg Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg 
            660                 665                 670         


Asp Lys Val Thr Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
        675                 680                 685             


Thr Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser 
    690                 695                 700                 


Phe Val Asp Glu Ile Arg Leu Ala Gln Gly Ser Gly Lys Ala Glu Asp 
705                 710                 715                 720 


Tyr Arg Ala Glu Val Gln Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Ile Glu Val Met 
            740                 745                 750         


Gly Tyr Asp Pro Glu His Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Lys Ile 
    770                 775                 780                 


Glu Asn Ala Ile Lys Asn Leu Asp Ser Asn Leu Asn Ser Lys Ile Leu 
785                 790                 795                 800 


Lys Glu Tyr Pro Thr Asn Asn Gln Ala Leu Gln Asn Asp Arg Leu Phe 
                805                 810                 815     


Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Glu Glu Leu 
            820                 825                 830         


Asp Ile Asp Gln Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln 
        835                 840                 845             


Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Thr Lys Ser 
    850                 855                 860                 


Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile Val 
865                 870                 875                 880 


Tyr Lys Lys Lys Asn Phe Trp Lys Gln Leu Leu Asp Ser Gln Leu Ile 
                885                 890                 895     


Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu 
            900                 905                 910         


Thr Asn Glu Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr 
        915                 920                 925             


Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Thr Arg Phe Asn 
    930                 935                 940                 


Thr Lys Leu Asp Glu Ala Gly Asn Arg Ile Arg Asp Pro Lys Val Asn 
945                 950                 955                 960 


Ile Met Thr Leu Lys Ser Ser Leu Val Ser Gln Phe Arg Lys Asp Tyr 
                965                 970                 975     


Gln Leu Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp 
            980                 985                 990         


Ala Tyr Leu Asn Ala Val Val Ala  Thr Ala Leu Leu Lys  Lys Tyr Pro 
        995                 1000                 1005             


Gln Leu  Ala Pro Glu Phe Val  Tyr Gly Asp Tyr Pro  Lys Tyr Asn 
    1010                 1015                 1020             


Ser Tyr  Lys Ser Arg Lys Ser  Ala Thr Glu Lys Val  Leu Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Arg Val Leu Val  Tyr Ser Lys 
    1040                 1045                 1050             


Thr Gly  Glu Val Arg Ile Arg  Pro Val Ile Glu Val  Asn Lys Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Lys Ser Asp Phe  Arg Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Tyr Pro  Gln Val Asn Val Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Asn His Gly Leu  Asp Arg Gly Lys Pro  Lys Gly Phe 
    1100                 1105                 1110             


Tyr Asn  Ala Asn Leu Ser Pro  Arg Pro Lys Glu Gly  Ser Val Glu 
    1115                 1120                 1125             


Asn Leu  Val Pro Ala Lys Gln  Ser Phe Asp Thr Lys  Arg Tyr Gly 
    1130                 1135                 1140             


Gly Tyr  Ala Gly Ile Ser Asn  Ser Tyr Ala Val Leu  Val Asn Gly 
    1145                 1150                 1155             


Ile Ile  Glu Lys Gly Lys Lys  Lys Ser Lys Ala Glu  Val Thr Glu 
    1160                 1165                 1170             


Phe Gln  Gly Ile Ser Ile Leu  Ala Arg Lys Asp Phe  Asp Lys Asn 
    1175                 1180                 1185             


Pro Lys  Gln Tyr Leu Leu Asn  Leu Gly Tyr Lys Asp  Ile Lys Ser 
    1190                 1195                 1200             


Ile Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Glu Asn Gly 
    1205                 1210                 1215             


Ser Arg  Arg Met Leu Ala Ser  Ile Leu Ser Thr Asn  Asn Lys Arg 
    1220                 1225                 1230             


Gly Glu  Ile His Lys Gly Asn  Glu Met Tyr Leu Pro  Asp Lys Phe 
    1235                 1240                 1245             


Val Thr  Leu Leu Tyr His Ala  Met Arg Val Asn Arg  Thr Leu Glu 
    1250                 1255                 1260             


Pro Gly  His Lys Lys Tyr Val  Glu Ala His Arg His  Val Phe Asp 
    1265                 1270                 1275             


Glu Leu  Leu Thr Tyr Ile Leu  Glu Tyr Asn Glu Lys  Ile Val Gly 
    1280                 1285                 1290             


Ala Lys  Ala Asn Gly Lys Arg  Ile Val Glu Ala Tyr  Ser Ala Arg 
    1295                 1300                 1305             


Lys Asp  Thr Asp Asn Leu Glu  Glu Leu Cys Asn Ser  Phe Ile Asn 
    1310                 1315                 1320             


Leu Leu  Asn Leu Thr Ala Leu  Gly Ser Ala Val Asp  Phe Glu Phe 
    1325                 1330                 1335             


Leu Gly  Thr Lys Ile Pro Arg  Tyr Arg Asp Tyr Thr  Pro Ser Ser 
    1340                 1345                 1350             


Leu Leu  Lys Ala Thr Leu Ile  His Gln Ser Val Thr  Gly Leu Tyr 
    1355                 1360                 1365             


Glu Thr  Arg Ile Asp Leu Ser  Lys Leu Gly Gly Asp  
    1370                 1375                 1380 


<210>  1093
<211>  1380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1093

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Tyr Glu Ser Glu Asn Ala Asp Glu Lys Ser Ser Ile Val 
            20                  25                  30          


Arg Leu Gly Val Arg Val Asn Pro Leu Ser Val Asp Glu Lys Gly Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Gln Gln Thr 
    50                  55                  60                  


His Gly Ala Arg Ile Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Asn 
65                  70                  75                  80  


Leu Lys Asp Cys Leu Gln Lys Gln Gly Trp Ile Gly Thr Glu Ala Leu 
                85                  90                  95      


Tyr Glu Glu Gly Lys Thr Ser Thr Phe Glu Thr Tyr Met Leu Arg Ala 
            100                 105                 110         


Lys Ala Ala Glu Glu Glu Ile Ser Leu His Glu Leu Cys Arg Val Leu 
        115                 120                 125             


Phe Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Asn Arg Lys Ala Asn 
    130                 135                 140                 


Asn Lys Glu Asp Gly Lys Leu Phe Asp Gly Met Thr Val Ala Lys Lys 
145                 150                 155                 160 


Leu Tyr Glu Glu His Leu Thr Pro Ala Glu Tyr Ser Leu Lys Leu Leu 
                165                 170                 175     


Ser Ser Gly Lys Lys Phe Lys Gln Glu Tyr Tyr Arg Ser Asp Leu Lys 
            180                 185                 190         


Glu Glu Phe Asp Arg Ile Trp Asn Glu Gln Gln Lys Tyr Tyr Pro Glu 
        195                 200                 205             


Ile Leu Thr Asp Asp Phe Lys Gln Gln Leu Gln Gly Lys Ser Lys Gly 
    210                 215                 220                 


Asn Thr Ser Lys Ile Phe Leu Gly Lys Tyr Gly Ile Tyr Ser Ala Asp 
225                 230                 235                 240 


Leu Lys Gly Leu Asp Arg Lys Leu Gln Pro Leu Lys Trp Arg Val Glu 
                245                 250                 255     


Ala Leu Lys Gln Lys Val Asp Lys Glu Val Leu Ala Phe Val Ile Ser 
            260                 265                 270         


Asp Leu Asn Gly Gln Ile Ala Ser Thr Ser Gly Leu Leu Gly Ala Ile 
        275                 280                 285             


Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln 
    290                 295                 300                 


Tyr Leu Trp Thr Ser Leu Lys Glu Ser Pro His Val Ser Ile Lys Asn 
305                 310                 315                 320 


Lys Pro Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Lys Ile Trp 
                325                 330                 335     


Glu Thr Gln Ala Thr Tyr His Lys Glu Leu Thr Pro Glu Leu Lys Gln 
            340                 345                 350         


Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
        355                 360                 365             


Lys Gly Leu Ile Ser Val Cys Glu Leu Glu Gln Arg Lys Val Lys Val 
    370                 375                 380                 


Thr Ile Asp Gly Lys Glu Lys Glu Ile Ser Ile Gly Pro Lys Val Ala 
385                 390                 395                 400 


Pro Lys Ser Ser Pro Met Phe Gln Glu Phe Arg Ile Trp Gln Asn Leu 
                405                 410                 415     


Asn Asn Ile Leu Leu Ile Asp Lys Gly Asn Asn Ser Asn Arg Pro Leu 
            420                 425                 430         


Tyr Glu Glu Glu Arg Asn Leu Leu Tyr Glu Glu Leu Ser Val Lys Glu 
        435                 440                 445             


Lys Leu Ser Lys Ala Glu Ala Leu Lys Val Leu Asn Lys Asn Gly Lys 
    450                 455                 460                 


Gln Trp Asp Leu Asn Tyr Lys Glu Ile Glu Gly Asn Arg Thr Gln Ala 
465                 470                 475                 480 


Val Leu Tyr Asp Cys Tyr Asn Arg Ile Ile Gly Met Thr Gly His Glu 
                485                 490                 495     


Glu Cys Asn Phe Lys Lys Leu Lys Ala Ser Asp Ile Arg Tyr Asn Val 
            500                 505                 510         


Lys Thr Ile Phe Lys Asn Leu Gly Phe Ser Thr Asp Ile Leu Asp Phe 
        515                 520                 525             


Asp Ser Ser Leu Gln Gly His Glu Leu Glu Arg Gln Pro Ser Tyr Gln 
    530                 535                 540                 


Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asn Ser Arg Thr Gly 
545                 550                 555                 560 


Asn Asp Ala Leu Leu Arg Lys Leu Glu Thr Ala Phe Ser Ile Pro Lys 
                565                 570                 575     


Glu Tyr Ala Ala Val Leu Ser Asp Val Val Phe Glu Asp Ser Tyr Gly 
            580                 585                 590         


Asn Leu Ser Val Lys Ala Met Arg Asn Ile Leu Pro His Leu Gln Ala 
        595                 600                 605             


Gly Leu Asp Tyr Ser Gln Ala Cys Glu Lys Ala Gly Tyr Lys His Ser 
    610                 615                 620                 


Arg Arg Ser Leu Thr Lys Asn Glu Leu Asp Lys Lys Glu Tyr Lys Glu 
625                 630                 635                 640 


Lys Leu Glu Leu Leu Pro Lys Ser Ser Leu His Asn Pro Val Val Glu 
                645                 650                 655     


Lys Ile Leu Asn Gln Met Ile Asn Val Ile Asn Ala Val Ile Asp Glu 
            660                 665                 670         


Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys 
        675                 680                 685             


Ile Gly Ala Ala Glu Arg Glu Asn Met Thr Arg Ser Ile Asn Gln Gly 
    690                 695                 700                 


Asn Ala Glu Asn Lys Arg Ile Arg Glu Ile Leu Glu Lys Glu Phe Ala 
705                 710                 715                 720 


Leu Ser Tyr Ile Ser Arg Asn Asp Ile Ile Lys Tyr Lys Leu Tyr Glu 
                725                 730                 735     


Glu Leu Lys Ser Asn Gly Phe Lys Thr Leu Tyr Ser Asp Thr Tyr Ile 
            740                 745                 750         


Ser Lys Asp Lys Leu Phe Ser Lys Asp Phe Asp Ile Glu His Ile Ile 
        755                 760                 765             


Pro Lys Ala Arg Leu Phe Asp Asp Ser Leu Ser Asn Lys Thr Ile Glu 
    770                 775                 780                 


Ala Arg Asp Val Asn Ile Ala Lys Ser Asn Lys Thr Ala Phe Asp Phe 
785                 790                 795                 800 


Ile Ser Glu Lys Tyr Asp Gln Gln Gly Ile Glu Ala Tyr Lys Lys Lys 
                805                 810                 815     


Leu Asp Ile Leu Leu Gln Asp Gly Ser Ile Ser Lys Ala Lys Tyr Asn 
            820                 825                 830         


Arg Leu Leu Met Lys Glu Ala Asp Ile Pro Ser Gly Phe Ile Asp Arg 
        835                 840                 845             


Asp Leu Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Tyr Glu Ile Leu 
    850                 855                 860                 


Ser Asp Leu Val Arg Asn Val Thr Pro Thr Thr Gly Ala Val Thr Ser 
865                 870                 875                 880 


Arg Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn 
                885                 890                 895     


Phe Glu Lys Tyr Gln Lys Leu Gly Leu Val Glu Val Ile Glu Asp Arg 
            900                 905                 910         


Asp Gly Arg Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp 
        915                 920                 925             


His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr Lys Pro 
    930                 935                 940                 


Ser Tyr Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Ser Asp Lys Asn 
945                 950                 955                 960 


Ser Ser Ile Tyr Gly Ile Glu Asn Lys Glu Leu His Arg Glu Asp Asn 
                965                 970                 975     


Lys Leu Arg Phe Asn Ala Pro Leu Pro Ile Asn Glu Phe Arg Ala Glu 
            980                 985                 990         


Ala Arg Arg His Leu Lys Ser Ile  Leu Val Ser Val Lys  Ala Lys Asn 
        995                 1000                 1005             


Lys Val  Met Thr Gln Asn Val  Asn Val Ile Lys Thr  Lys His Gly 
    1010                 1015                 1020             


Val Ile  Lys Lys Val Gln Leu  Thr Pro Arg Gly Glu  Leu His Asn 
    1025                 1030                 1035             


Ala Thr  Phe Tyr Gly Thr Lys  Lys Arg Pro Ile Ile  Lys Met Val 
    1040                 1045                 1050             


Lys Val  Gly Ala Ala Leu Asp  Glu Val Thr Ile Lys  Lys Val Ser 
    1055                 1060                 1065             


Ser Ser  Phe Ile Arg Glu Ala  Leu Leu Asp Arg Leu  His Glu Tyr 
    1070                 1075                 1080             


Asn Gly  Asp Ala Lys Lys Ala  Phe Thr Gly Arg Asn  Ser Ile Asp 
    1085                 1090                 1095             


Lys Asn  Pro Ile Tyr Ile Asp  Glu Glu His Lys Lys  Thr Val Pro 
    1100                 1105                 1110             


Ala Leu  Val Lys Thr Ile Glu  Trp Glu Met Tyr His  Pro Thr Arg 
    1115                 1120                 1125             


Lys Met  Ile Ser Pro Asp Leu  Lys Ile Asp Lys Val  Ile Asp Lys 
    1130                 1135                 1140             


Gly Ile  Arg Glu Ile Leu Arg  Thr Arg Leu Ala Lys  Phe Asn Gly 
    1145                 1150                 1155             


Asp Ala  Gln Lys Ala Phe Ser  Asn Leu Asp Glu Asn  Pro Ile Tyr 
    1160                 1165                 1170             


Lys Asp  Glu Ala Lys Lys Ile  Thr Leu Lys Arg Val  Ser Leu Glu 
    1175                 1180                 1185             


Gly Val  Ser Ser Val Ile Pro  Leu His Lys Leu Lys  Asp Met Gly 
    1190                 1195                 1200             


Gly Lys  Pro Val Val Gly Lys  Asp Gly Lys Pro Val  Trp Gly Asp 
    1205                 1210                 1215             


Tyr Val  Gln Thr Ser Asn Asn  His His Ile Ala Phe  Tyr Met Asp 
    1220                 1225                 1230             


Glu Asn  Gly Asn Leu Gln Asp  Asp Val Val Thr Phe  Phe Lys Ala 
    1235                 1240                 1245             


Ala Arg  Arg Lys Ser Gln Gly  Val Pro Val Ile Asp  Lys Asn Tyr 
    1250                 1255                 1260             


Asn His  Asp Lys Gly Trp His  Phe Leu Phe Thr Met  Lys Gln Asn 
    1265                 1270                 1275             


Glu Tyr  Phe Val Phe Pro Asn  Glu Ala Thr Gly Phe  Ile Pro Thr 
    1280                 1285                 1290             


Glu Val  Asp Leu Met Asp Glu  Ala Asn Tyr Asp Met  Ile Ser Pro 
    1295                 1300                 1305             


Asn Leu  Tyr Arg Val Gln Lys  Met Ser Lys Thr Lys  Gln Gly Ile 
    1310                 1315                 1320             


Thr Asp  Lys Arg Asp Tyr Trp  Phe Arg His His Leu  Glu Thr Val 
    1325                 1330                 1335             


Leu Asn  Asn Asp Leu Lys Val  Arg Asn Ser Ile Phe  Lys Gln Ile 
    1340                 1345                 1350             


Asn Ser  Leu Lys Pro Leu Glu  Gly Ile Val Lys Val  Arg Ile Asn 
    1355                 1360                 1365             


Ala Ile  Gly Lys Ile Val Ala  Ile Gly Glu Tyr Asp  
    1370                 1375                 1380 


<210>  1094
<211>  1380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1094

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Ser Asn Asn Glu Ala Ser Ser Ile Val 
            20                  25                  30          


Arg Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Lys Ser Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ala Ile Thr Thr Asn Ala Asp Arg Gln Leu Arg 
    50                  55                  60                  


His Gly Ala Arg Ile Asn Leu Gln Arg Tyr Lys Leu Arg Arg Gln Asn 
65                  70                  75                  80  


Leu His Asp Cys Leu Gln Lys Gln Gly Trp Leu Gly Thr Glu Ala Met 
                85                  90                  95      


Tyr Glu Glu Gly Lys Ala Ser Thr Phe Glu Thr Tyr Lys Leu Arg Ala 
            100                 105                 110         


Lys Ala Ala Glu Glu Glu Ile Ser Leu His Glu Phe Ala Arg Val Leu 
        115                 120                 125             


Phe Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Asn Arg Lys Ala Asn 
    130                 135                 140                 


Asn Lys Glu Asp Gly Gln Leu Phe Asp Gly Met Thr Ile Ala Lys Lys 
145                 150                 155                 160 


Leu Tyr Glu Glu His Leu Thr Pro Ala Glu Tyr Ser Leu Gln Leu Leu 
                165                 170                 175     


Asn Lys Gly Lys Lys Phe Thr Gln Gly Tyr Tyr Arg Ser Asp Leu Asn 
            180                 185                 190         


Ala Glu Leu Glu Arg Ile Trp Asp Glu Gln Lys Lys Tyr Tyr Pro Glu 
        195                 200                 205             


Ile Leu Thr Asp Glu Phe Lys Gln Gln Leu Glu Gly Lys Thr Lys Thr 
    210                 215                 220                 


Asn Thr Ser Lys Ile Phe Leu Ala Lys Tyr Gly Ile Tyr Ser Ala Asp 
225                 230                 235                 240 


Leu Lys Gly Leu Asp Arg Lys Leu Gln Pro Leu Lys Trp Arg Val Glu 
                245                 250                 255     


Ala Leu Lys Gln Gln Val Asp Lys Glu Val Leu Ala Phe Val Ile Ser 
            260                 265                 270         


Asp Leu Lys Gly Gln Ile Ala Asn Thr Ser Gly Leu Leu Gly Ala Ile 
        275                 280                 285             


Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln 
    290                 295                 300                 


Tyr Leu Trp Ala Ser Leu Glu Glu Asn Pro His Ile Ser Ile Lys Asn 
305                 310                 315                 320 


Lys Pro Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Lys Ile Trp 
                325                 330                 335     


Glu Thr Gln Ala Thr Phe His Lys Gln Leu Thr Pro Glu Leu Lys Gln 
            340                 345                 350         


Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
        355                 360                 365             


Lys Ser Leu Ile Ser Val Cys Glu Leu Glu Gln Arg Lys Val Lys Ala 
    370                 375                 380                 


Thr Ile Asp Gly Lys Glu Lys Glu Ile Thr Ile Gly Pro Lys Val Ala 
385                 390                 395                 400 


Pro Lys Ser Ser Pro Val Phe Gln Glu Phe Arg Ile Trp Gln Asn Leu 
                405                 410                 415     


Asn Asn Val Leu Leu Ile Asp Asn Asp Thr Asn Glu Lys Arg Pro Leu 
            420                 425                 430         


Asp Glu Asp Glu Arg Tyr Leu Leu Tyr Lys Glu Leu Ser Ile Lys Ala 
        435                 440                 445             


Lys Leu Ser Lys Thr Glu Ala Leu Lys Ile Leu Asn Lys Lys Gly Lys 
    450                 455                 460                 


Gln Trp Asp Leu Asn Tyr Lys Glu Leu Glu Gly Asn Arg Thr Gln Ala 
465                 470                 475                 480 


Ile Leu Phe Asp Cys Tyr Asn Arg Ile Ile Thr Leu Thr Gly His Glu 
                485                 490                 495     


Glu Cys Asp Phe Lys Lys Ile Lys Ala Ser Glu Ile Arg His Tyr Val 
            500                 505                 510         


Ser Thr Ile Phe Lys Asn Leu Gly Phe Ser Thr Glu Ile Leu Asp Phe 
        515                 520                 525             


Asp Pro Ser Leu Glu Lys His Glu Leu Glu Lys Gln Pro Met Tyr Gln 
    530                 535                 540                 


Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asn Ser Arg Thr Gly 
545                 550                 555                 560 


Asn Glu Ser Leu Leu Arg Lys Leu Glu Thr Thr Phe Gly Phe Pro Glu 
                565                 570                 575     


Glu Tyr Ala Thr Val Leu Cys Asp Val Val Phe Glu Glu Asp Tyr Gly 
            580                 585                 590         


Asn Leu Ser Val Lys Ala Met Arg Lys Ile Leu Pro Tyr Leu Gln Ala 
        595                 600                 605             


Gly Asn Asp Tyr Ser Gln Ala Cys Ala Tyr Ala Gly Tyr Asn His Ser 
    610                 615                 620                 


Arg His Ser Leu Thr Lys Glu Glu Leu Asp Gln Lys Val Tyr Lys Glu 
625                 630                 635                 640 


Arg Leu Glu Leu Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
                645                 650                 655     


Lys Ile Leu Asn Gln Met Ile Asn Val Ile Asn Ala Ile Ile Asp Glu 
            660                 665                 670         


Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Met Ala Arg Glu Leu Lys 
        675                 680                 685             


Ser Ser Ala Ala Asp Arg Lys Lys Thr Thr His Ala Ile Asn Gln Gly 
    690                 695                 700                 


Asn Asp Glu Asn Gln Cys Ile Arg Glu Ile Leu Glu Lys Glu Phe Ala 
705                 710                 715                 720 


Leu Ser Tyr Ile Ser Arg Asn Asp Ile Ile Lys Tyr Lys Leu Tyr Glu 
                725                 730                 735     


Glu Leu Lys Pro Asn Gly Phe Lys Thr Leu Tyr Ser Asp Thr Tyr Ile 
            740                 745                 750         


Pro Lys Asp Lys Leu Phe Ser Lys Asp Phe Asp Ile Glu His Ile Ile 
        755                 760                 765             


Pro Lys Ala Arg Leu Phe Asp Asp Ser Tyr Ser Asn Lys Thr Leu Glu 
    770                 775                 780                 


Ala Arg Asp Ile Asn Leu Ala Lys Ser Asn Lys Thr Ala Phe Asp Phe 
785                 790                 795                 800 


Val Lys Glu Lys Tyr Gly Glu Gln Gly Ala Glu Ala Tyr Lys Lys Lys 
                805                 810                 815     


Leu Asp Met Leu Leu Gly Asn Asn Val Ile Ser Lys Pro Lys Tyr Asn 
            820                 825                 830         


Lys Leu Leu Met Thr Glu Ala Asp Ile Pro Ser Asp Phe Ile Asn Arg 
        835                 840                 845             


Asp Leu Arg Asn Thr Gln Tyr Ile Ala Arg Lys Ala Cys Glu Ile Leu 
    850                 855                 860                 


Gly Glu Leu Val Arg Thr Val Thr Pro Thr Thr Gly Lys Ile Thr Asn 
865                 870                 875                 880 


Arg Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn 
                885                 890                 895     


Phe Glu Lys Tyr Glu Lys Leu Gly Leu Thr Glu Ile Val Glu Asp Arg 
            900                 905                 910         


Asp Gly Arg Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp 
        915                 920                 925             


His Arg His His Ala Met Asp Ala Leu Ala Ile Ala Phe Thr Lys Pro 
    930                 935                 940                 


Ser Tyr Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Ser Asn Lys Gly 
945                 950                 955                 960 


Asp Ser Ile Tyr Ala Ile Glu Asn Lys Glu Leu His Tyr Glu Glu Gly 
                965                 970                 975     


Lys Leu Arg Phe Asn Ala Pro Ile Pro Val Asn Val Phe Arg Ala Glu 
            980                 985                 990         


Ala Lys Arg His Leu Ser Ala Ile  Leu Val Ser Ile Lys  Ala Lys Asn 
        995                 1000                 1005             


Lys Val  Met Thr Gln Asn Val  Asn Lys Ile Lys Thr  Lys His Gly 
    1010                 1015                 1020             


Ile Ile  Lys Lys Ile Gln Leu  Thr Pro Arg Gly Pro  Leu His Asn 
    1025                 1030                 1035             


Glu Thr  Ile Tyr Gly Thr Lys  Met Arg Pro Ile Ile  Lys Lys Val 
    1040                 1045                 1050             


Lys Val  Gly Ala Ala Leu Asp  Glu Ala Thr Ile Asn  Lys Val Ser 
    1055                 1060                 1065             


Ser Pro  Ala Ile Arg Glu Val  Leu Leu Lys Arg Leu  Asn Glu Tyr 
    1070                 1075                 1080             


Ser Gly  Asn Ala Lys Lys Ala  Phe Thr Gly Lys Asn  Thr Leu Asp 
    1085                 1090                 1095             


Lys Asn  Pro Ile Tyr Leu Asn  Ala Glu Arg Thr Lys  Thr Val Pro 
    1100                 1105                 1110             


Ala Ser  Val Lys Thr Val Glu  Trp Glu Asn Tyr His  Pro Ile Arg 
    1115                 1120                 1125             


Lys Pro  Ile Thr Pro Asp Leu  Lys Val Asp Lys Val  Ile Asp Lys 
    1130                 1135                 1140             


Gly Ile  Gly Glu Ile Leu Lys  Ala Arg Leu Glu Glu  Phe Asn Gly 
    1145                 1150                 1155             


Asp Ala  Lys Lys Ala Phe Ser  Asn Leu Glu Glu Asn  Pro Ile Tyr 
    1160                 1165                 1170             


Leu Asp  Gln Thr Lys Lys Ile  Ala Leu Lys Arg Val  Ser Ile Glu 
    1175                 1180                 1185             


Gly Val  Leu Ser Thr Ile Pro  Leu His Thr Leu Lys  Asn Gln Ala 
    1190                 1195                 1200             


Gly Lys  Pro Ile Met Gly Lys  Asp Gly Lys Pro Val  Leu Gly Asn 
    1205                 1210                 1215             


Tyr Val  Gln Thr Ser Asn Asn  His His Ile Ala Phe  Tyr Tyr Asp 
    1220                 1225                 1230             


Glu Asp  Gly Asn Leu Gln Asp  Asn Ala Val Ser Phe  Phe Glu Ala 
    1235                 1240                 1245             


Ala Glu  Arg Lys Ser Gln Gly  Ile Ser Val Ile Asp  Lys Asp Tyr 
    1250                 1255                 1260             


Asn Arg  Asp Lys Gly Trp Arg  Phe Leu Phe Thr Met  Lys Gln Asn 
    1265                 1270                 1275             


Glu Tyr  Phe Val Phe Pro Asn  Glu Ala Thr Gly Phe  Ile Pro Ser 
    1280                 1285                 1290             


Glu Val  Asp Leu Thr Asp Glu  Ala Asn Tyr Gly Ile  Ile Ser Pro 
    1295                 1300                 1305             


Asn Leu  Tyr Arg Val Gln Lys  Met Ser Arg Ile Glu  Lys Gly Thr 
    1310                 1315                 1320             


Ser Val  Ser Arg Asp Tyr Trp  Phe Arg His His Leu  Glu Thr Ile 
    1325                 1330                 1335             


Leu Asn  Asp Asp Ser Arg Leu  Lys Asn Ser Ala Phe  Lys Arg Ile 
    1340                 1345                 1350             


Asn Ser  Leu Lys Pro Leu Glu  Gly Ile Ile Lys Val  Arg Ile Asn 
    1355                 1360                 1365             


Ser Ile  Gly Lys Ile Val Ala  Val Gly Glu Tyr Asp  
    1370                 1375                 1380 


<210>  1095
<211>  1380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1095

Met Lys Lys Gly Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Phe Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Arg Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Ala Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser Ser 
                85                  90                  95      


Phe Phe His Arg Leu Asp Asp Ser Phe Leu Ile Pro Glu Asp Lys Arg 
            100                 105                 110         


Glu Ser Lys Tyr Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Glu Tyr 
        115                 120                 125             


His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ser Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Asp Thr Phe Asp Ile 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         


Asp Asn Ala Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             


Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 


Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     


Asp Leu Glu Asp Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             


Leu Phe Val Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Glu Arg Tyr Glu Asn His Gln Asn Asp Leu Val Val Leu Lys 
                325                 330                 335     


Gln Phe Ile Lys Asn Asn Leu Pro Glu Asn Tyr Val Glu Val Phe Ser 
            340                 345                 350         


Asp Asp Ser Lys Asn Gly Tyr Ala Gly Tyr Val Gly Val Asp Ser Lys 
        355                 360                 365             


Ile Arg Lys Arg Asn Ala Lys Leu Ala Thr Glu Glu Glu Phe Tyr Lys 
    370                 375                 380                 


Tyr Leu Lys Asn Ile Leu Asn Asn Val Lys Gly Ala Asp Tyr Phe Leu 
385                 390                 395                 400 


Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg Lys Gln Arg Thr Phe Asp 
                405                 410                 415     


Asn Gly Ser Ile Pro His Gln Ile His Leu Gln Glu Met Asn Ala Ile 
            420                 425                 430         


Leu Arg Arg Gln Gly Glu His Tyr Pro Phe Leu Lys Glu Asn Arg Glu 
        435                 440                 445             


Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
    450                 455                 460                 


Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp Leu Thr Arg Asn Ser Asp 
465                 470                 475                 480 


Gln Ala Ile Arg Pro Trp Asn Phe Glu Glu Ile Val Asp Lys Ala Ser 
                485                 490                 495     


Ser Ala Glu Asp Phe Ile Asn Lys Met Thr Asn Tyr Asp Leu Tyr Leu 
            500                 505                 510         


Pro Glu Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Lys Phe 
        515                 520                 525             


Ala Val Tyr Asn Glu Leu Thr Lys Val Lys Phe Ile Ala Glu Gly Leu 
    530                 535                 540                 


Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln Lys Lys Gln Ile Val Asn 
545                 550                 555                 560 


Gln Leu Phe Lys Glu Lys Arg Lys Val Thr Glu Lys Asp Ile Ile His 
                565                 570                 575     


Tyr Leu His Thr Val Asp Gly Tyr Asp Gly Ile Glu Leu Lys Gly Ile 
            580                 585                 590         


Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr Tyr His Asp Leu Leu Lys 
        595                 600                 605             


Ile Ile Lys Asp Lys Glu Phe Met Asp Asp Pro Lys Asn Glu Glu Ile 
    610                 615                 620                 


Leu Glu Asn Ile Val His Thr Leu Thr Ile Phe Glu Asp Arg Glu Met 
625                 630                 635                 640 


Ile Lys Gln Arg Leu Ala Gln Tyr Asp Ser Leu Phe Asp Glu Lys Val 
                645                 650                 655     


Ile Lys Ser Leu Thr Arg Arg His Tyr Thr Gly Trp Gly Lys Leu Ser 
            660                 665                 670         


Ala Met Leu Ile Asn Gly Ile Arg Asp Lys Gln Thr Gly Lys Thr Ile 
        675                 680                 685             


Leu Asp Tyr Leu Ile Asp Asp Ala Tyr Tyr Asn Arg Asn Phe Met Gln 
    690                 695                 700                 


Leu Ile Asn Asp Asp Gly Leu Ser Phe Lys Glu Ile Ile Gln Lys Ala 
705                 710                 715                 720 


Gln Val Val Gly Lys Thr Asp Asp Val Lys Gln Val Val Gln Glu Leu 
                725                 730                 735     


Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Ser Ile Lys Ile 
            740                 745                 750         


Val Asp Glu Leu Val Lys Ile Met Arg His Asn Pro Glu Ser Ile Val 
        755                 760                 765             


Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Ala Arg Gly Lys Lys Asn 
    770                 775                 780                 


Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp Ala Leu Lys Asn Leu Ala 
785                 790                 795                 800 


Pro Gly Leu Asp Ser Asn Ile Leu Lys Glu His Pro Thr Asp Asn Val 
                805                 810                 815     


Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn Gly Lys 
            820                 825                 830         


Asp Met Tyr Thr Gly Glu Ala Leu Asp Ile Asn Gln Leu Ser Ser Tyr 
        835                 840                 845             


Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp Ser Phe 
    850                 855                 860                 


Asp Asn Arg Val Leu Thr Ser Ser Lys Asp Asn Arg Gly Lys Ser Asp 
865                 870                 875                 880 


Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys Ala Phe Trp Gln 
                885                 890                 895     


Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys Phe Asn Asn Leu 
            900                 905                 910         


Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu Arg Asp Lys Val Gly Phe 
        915                 920                 925             


Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala 
    930                 935                 940                 


Gln Ile Leu Asp Ala Arg Tyr Asn Thr Glu Val Asn Glu Lys Asp Lys 
945                 950                 955                 960 


Lys Asn Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Asn Leu Val Ser 
                965                 970                 975     


Asn Phe Arg Lys Glu Phe Arg Leu Tyr Lys Val Arg Glu Ile Asn Asp 
            980                 985                 990         


Tyr His His Ala His Asp Ala Tyr  Leu Asn Ala Val Val  Ala Lys Ala 
        995                 1000                 1005             


Ile Leu  Lys Lys Tyr Pro Lys  Leu Glu Pro Glu Phe  Val Tyr Gly 
    1010                 1015                 1020             


Glu Tyr  Gln Lys Tyr Asp Leu  Lys Arg Tyr Ile Ser  Arg Ser Lys 
    1025                 1030                 1035             


Asp Pro  Lys Glu Val Glu Lys  Ala Thr Glu Lys Tyr  Phe Phe Tyr 
    1040                 1045                 1050             


Ser Asn  Leu Leu Asn Phe Phe  Lys Glu Glu Val His  Tyr Ala Asp 
    1055                 1060                 1065             


Gly Thr  Ile Val Lys Arg Glu  Asn Ile Glu Tyr Ser  Lys Asp Thr 
    1070                 1075                 1080             


Gly Glu  Ile Ala Trp Asn Lys  Glu Lys Asp Phe Ala  Ile Ile Lys 
    1085                 1090                 1095             


Lys Val  Leu Ser Leu Pro Gln  Val Asn Ile Val Lys  Lys Arg Glu 
    1100                 1105                 1110             


Val Gln  Thr Gly Gly Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly 
    1115                 1120                 1125             


Asn Ser  Asp Lys Leu Ile Pro  Arg Lys Thr Lys Asp  Ile Leu Trp 
    1130                 1135                 1140             


Asp Thr  Thr Lys Tyr Gly Gly  Phe Asp Ser Pro Val  Ile Ala Tyr 
    1145                 1150                 1155             


Ser Ile  Leu Leu Ile Ala Asp  Ile Glu Lys Gly Lys  Ala Lys Lys 
    1160                 1165                 1170             


Leu Lys  Thr Val Lys Thr Leu  Val Gly Ile Thr Ile  Met Glu Lys 
    1175                 1180                 1185             


Ala Ala  Phe Glu Asp Asn Pro  Ile Thr Phe Leu Glu  Asn Lys Gly 
    1190                 1195                 1200             


Tyr His  Asn Val Arg Lys Glu  Asn Ile Leu Cys Leu  Pro Lys Tyr 
    1205                 1210                 1215             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Arg Arg Leu  Leu Ala Ser 
    1220                 1225                 1230             


Ala Lys  Glu Leu Gln Lys Gly  Asn Glu Ile Val Leu  Pro Val Tyr 
    1235                 1240                 1245             


Leu Thr  Thr Leu Leu Tyr His  Ser Lys Asn Val His  Lys Leu Asp 
    1250                 1255                 1260             


Glu Pro  Gly His Leu Glu Tyr  Ile Gln Lys His Arg  Asn Glu Phe 
    1265                 1270                 1275             


Lys Asp  Leu Leu Asn Leu Val  Ser Glu Phe Ser Gln  Lys Tyr Val 
    1280                 1285                 1290             


Leu Ala  Asp Ala Asn Leu Glu  Lys Ile Lys Ser Leu  Tyr Ala Asp 
    1295                 1300                 1305             


Asn Glu  Gln Ala Asp Ile Glu  Ile Leu Ala Asn Ser  Phe Ile Asn 
    1310                 1315                 1320             


Leu Leu  Thr Phe Thr Ala Leu  Gly Ala Pro Ala Ala  Phe Lys Phe 
    1325                 1330                 1335             


Phe Gly  Lys Asp Ile Asp Arg  Lys Arg Tyr Thr Thr  Val Ser Glu 
    1340                 1345                 1350             


Ile Leu  Asn Ala Thr Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr 
    1355                 1360                 1365             


Glu Thr  Arg Ile Asp Leu Ser  Lys Leu Gly Glu Asp  
    1370                 1375                 1380 


<210>  1096
<211>  1380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1096

Met Asn Leu Arg His Val Gly Asn Tyr Asn Ile Gly Leu Asp Met Gly 
1               5                   10                  15      


Thr Ala Ser Val Gly Trp Ala Ala Ile Asp Glu Gln Gly Glu Leu Leu 
            20                  25                  30          


His Phe Lys Gly Lys Pro Thr Trp Gly Ser Arg Leu Phe Asp Ser Ala 
        35                  40                  45              


Asn Thr Ala Ala Thr Ala Arg Met Pro Arg Gly Gln Arg Arg Arg Tyr 
    50                  55                  60                  


Ile Arg Arg Arg Trp Arg Leu Asp Leu Leu Gln Lys Phe Phe Ser Glu 
65                  70                  75                  80  


Glu Met Ala Glu Lys Asp Pro Glu Phe Phe Ile Arg Leu Arg Gln Ser 
                85                  90                  95      


Ser Leu Trp Pro Glu Asp Arg Glu Gly Gly His Thr Ala Tyr His Trp 
            100                 105                 110         


Pro Leu Phe Asn Asp Ser Asp Phe Asn Glu Lys Glu Tyr Tyr Glu Lys 
        115                 120                 125             


Phe Pro Thr Ile Phe His Leu Arg Gln Trp Leu Met Glu Thr Asp Glu 
    130                 135                 140                 


Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Phe His Asn Ile Val Lys 
145                 150                 155                 160 


His Arg Gly Asn Phe Leu Gln Glu Asn Asn Asp Ser Leu Ser Ala Gln 
                165                 170                 175     


Asn Ala Asn Met Arg Glu Ser Leu Glu Ser Phe Cys Ile Ala Leu Glu 
            180                 185                 190         


Glu Trp Cys Asp Glu Lys Glu Leu Thr Cys Ser Cys Asp Pro Gln Lys 
        195                 200                 205             


Leu Glu His Leu Phe Lys Glu Val Lys Tyr Arg Ser Asp Ile Arg Asp 
    210                 215                 220                 


Gly Phe Ala Ala Leu Leu Gly Leu Ala Cys Ser Gly Glu Asn Glu Val 
225                 230                 235                 240 


Asp Val Lys Ala Met Ala Lys Glu Met Gly Lys Ala Ile Val Gly Tyr 
                245                 250                 255     


Lys Ala Asp Phe Ser Lys Ile Phe Thr Glu Cys Asp Lys Glu Gly Ser 
            260                 265                 270         


Ser Phe Arg Leu Ser Asp Asp Glu Gly Pro Glu Ala Phe Leu Asp Ile 
        275                 280                 285             


Cys Pro Asp Asp Gly Ile Ala Leu Phe Glu Ala Ile Gln Met Val Tyr 
    290                 295                 300                 


Ser Ser Phe Ile Leu Thr Gly Met Leu Thr Glu Lys Gly Leu Ser Leu 
305                 310                 315                 320 


Ser Lys Ile Ser Ala Tyr Asn Gln Tyr Gly Gln Asp Leu Glu Ser Leu 
                325                 330                 335     


Lys Ala Leu Val Lys Glu Tyr Val Pro Asn Glu Tyr Ser Ser Phe Phe 
            340                 345                 350         


Arg Gly Pro Thr Tyr Ser Gly Ser Thr Glu Tyr Asp Pro Ser Lys Ala 
        355                 360                 365             


Gln Gly Tyr Thr Arg Tyr Asn Leu Gly Pro Ser Lys Lys Gly Ser Gly 
    370                 375                 380                 


Ala Lys Pro Met Ala Tyr Glu Asp Phe Lys Lys Ser Val Glu Gln Leu 
385                 390                 395                 400 


Phe Ala Asn Thr Asn Ala Ile Asn Asp Glu Arg Tyr Lys Glu Met Met 
                405                 410                 415     


Gly Arg Phe Ala Glu Glu Lys Phe Leu Arg Arg Leu Arg Thr Ser Asp 
            420                 425                 430         


Asn Gly Arg Ile Pro Phe Gln Leu Asn Leu Glu Glu Met Thr Ala Ile 
        435                 440                 445             


Ile Ile Asn Gln Gly Lys Tyr Tyr Pro Phe Leu Leu Glu Gln Gln Glu 
    450                 455                 460                 


Lys Leu Glu Ser Leu Val Arg Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
465                 470                 475                 480 


Leu Thr Arg Ile Asn Ala Arg Leu Asp Ser Lys Gly Asn Ala Cys Phe 
                485                 490                 495     


Ala Trp Ser Lys Arg Lys Asp Gly Met Asp Ser Ala Ser Ile Lys Pro 
            500                 505                 510         


Trp Asn Trp Asp Glu Val Ile Asp Lys His Ala Ser Ala Gln Ala Phe 
        515                 520                 525             


Ile Glu Arg Met Thr Gly Thr Cys Thr Tyr Leu Arg Gly Glu Pro Val 
    530                 535                 540                 


Leu Pro Arg Cys Ser Leu Leu Tyr Glu Glu Tyr Cys Val Leu Asn Glu 
545                 550                 555                 560 


Leu Asn Gly Ala Arg Trp Thr Gln Asp Gly Asp Lys Phe Ile Arg Phe 
                565                 570                 575     


Asp Tyr Lys Asp Arg Ser Asp Ile Ile Glu Glu Leu Phe Lys Lys Arg 
            580                 585                 590         


Arg Gly Arg Ile Gln Tyr Ala Ala Val Ala Asp Trp Met Lys Gln Glu 
        595                 600                 605             


Arg Ser His Ala Asn Val His Val Ser Gly Gly Gln Gly Glu Asn Ala 
    610                 615                 620                 


Phe Glu Ser Arg Leu Ser Ser Tyr His Phe Phe Cys Asn Asp Val Phe 
625                 630                 635                 640 


Gly Val Asp Glu Leu Pro Gly Ser Tyr Val Asp Met Val Glu Glu Ile 
                645                 650                 655     


Ile Leu Trp Ser Thr Leu Phe Glu Asp Arg Ser Ile Leu Arg Glu Glu 
            660                 665                 670         


Ile Gln Arg Lys Tyr Gly Asp Val Leu Asn Glu Glu Gln Ile Lys Thr 
        675                 680                 685             


Ile Cys Lys Lys Arg Phe Ser Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    690                 695                 700                 


Leu Cys Ser Leu Lys Ala Gln Thr Asp Asn Gly Pro Lys Ser Ile Met 
705                 710                 715                 720 


Asp Ile Leu Gln Glu Gly Asp Gln Asn Asn Gly Arg Val Gly Arg Ala 
                725                 730                 735     


Met Val Phe Gln Glu Ile Leu His Asp Glu Gln Leu Gly Phe Glu Gln 
            740                 745                 750         


Lys Ile Glu Glu Glu Asn Thr Gln Ser Met Leu Glu Gly Glu Asn Leu 
        755                 760                 765             


Leu Ile Glu Asp Leu Pro Gly Ser Pro Ala Ile Arg Arg Ser Ile Asn 
    770                 775                 780                 


Gln Ala Val Arg Ile Val Asp Glu Ile Val Gly Ile Ala Gly His Ala 
785                 790                 795                 800 


Pro Asp Asn Ile Phe Ile Glu Val Thr Arg Glu Asp Asp Thr Lys Lys 
                805                 810                 815     


Lys Gly Lys Arg Thr Thr Thr Arg Tyr Ser Asn Ile Gln Lys Ala Leu 
            820                 825                 830         


Glu Ala Leu Lys Glu Glu Gly Ala Glu Ala Leu Arg Glu Leu Lys Thr 
        835                 840                 845             


Tyr Lys His Asn Glu Leu Asp Lys Arg Leu Ser Leu Tyr Phe Met Gln 
    850                 855                 860                 


Asn Gly Lys Ser Leu Tyr Ser Gly Ile Pro Leu Asp Ile Arg Arg Leu 
865                 870                 875                 880 


Ser Glu Tyr Gln Ile Asp His Ile Ile Pro Gln Ser Tyr Ile Lys Asp 
                885                 890                 895     


Asp Ser Tyr Glu Asn Met Ala Leu Val Leu Pro Gly Glu Asn Gln Ser 
            900                 905                 910         


Lys Ala Asp Asn Leu Leu Ile Ser Gln Asp Ile Arg Ile Lys Met Lys 
        915                 920                 925             


Ala Thr Trp Thr Ala Leu His Lys Ala Gly Leu Met Gly Asp Lys Lys 
    930                 935                 940                 


Tyr Asn Asn Leu Met Asn Ser Ser Ile Ser Asp Ala Lys Met Lys Gly 
945                 950                 955                 960 


Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Ser Val Lys Leu Thr 
                965                 970                 975     


Gln Met Met Leu Ser Gln Lys Tyr Pro Gly Ala Lys Val Arg Pro Ile 
            980                 985                 990         


Lys Ala Ser Ile Ser Ser Gln Leu  Arg Asp Ala Cys Asp  Leu Val Lys 
        995                 1000                 1005             


Cys Arg  Glu Ala Asn Asp Tyr  His His Ala His Asp  Ala Tyr Leu 
    1010                 1015                 1020             


Ala Cys  Gln Val Gly Arg Phe  Val Leu Lys Arg His  Ser Ser Met 
    1025                 1030                 1035             


Phe Asp  Asn Pro Ile Gly Met  Thr Lys Val Ile Arg  Asp Phe Val 
    1040                 1045                 1050             


Lys Arg  Gln Gly Gln Glu Phe  Lys Arg Asn Gly Lys  Leu Pro Ala 
    1055                 1060                 1065             


Tyr Ala  Ser Arg Ser Gly Phe  Ile Val Gln Ser Phe  Met Arg Ser 
    1070                 1075                 1080             


Gly Phe  Asp Ala Glu Thr Gly  Glu Leu Leu Arg Asp  Asp Trp Asn 
    1085                 1090                 1095             


Ala Pro  Phe Glu Ile Ala Arg  Ile Lys Arg Cys Leu  Asn Tyr Arg 
    1100                 1105                 1110             


Asp Cys  Tyr Ile Ser Arg Met  Pro Met Glu Asp Ser  Gly Ala Phe 
    1115                 1120                 1125             


Trp Asp  Ala Thr Ile Tyr Ser  Pro Lys Ala Gly Lys  Lys Gly Ala 
    1130                 1135                 1140             


Leu Pro  Val Lys Gln Gly Leu  Pro Val Glu Lys Tyr  Gly Ser Tyr 
    1145                 1150                 1155             


Ser Arg  Glu Gln Phe Ala Tyr  Phe Phe Val Tyr Lys  Ala Tyr Asn 
    1160                 1165                 1170             


Pro Lys  Lys Lys Ala Tyr Arg  Leu Glu Phe Glu Ala  Val Pro Val 
    1175                 1180                 1185             


Arg Val  Ala Ser Ala Val Glu  Thr Arg Glu Asn Ala  Leu Glu Glu 
    1190                 1195                 1200             


Tyr Ala  Lys Ser Val Ala Lys  Gln Ala Gly Leu Glu  Phe Val Glu 
    1205                 1210                 1215             


Val Ala  Arg Pro Lys Val Tyr  Lys Tyr Gln Leu Ile  Glu Leu Gly 
    1220                 1225                 1230             


Glu Asp  Arg Leu Tyr Ile Thr  Gly Lys Arg Glu Val  Arg Asn Ala 
    1235                 1240                 1245             


Ser Gln  Leu Ala Phe Ser Leu  Asp Glu Ile Asp Leu  Leu Lys Arg 
    1250                 1255                 1260             


Leu Ala  Glu Gly Glu Glu Thr  Ser Ser Glu Glu Arg  Glu Arg Leu 
    1265                 1270                 1275             


Phe Asp  Val Leu Ile Glu Lys  Tyr Ala Arg Tyr Ala  Pro Arg Leu 
    1280                 1285                 1290             


Ala Arg  Gln Leu Ser Val Asp  Ser Met Ser Ala Thr  Phe Glu Thr 
    1295                 1300                 1305             


Leu Asn  Ala Glu Glu Gln Leu  Gly Ile Leu Leu Ser  Leu Val Glu 
    1310                 1315                 1320             


Ile Ala  Asn Gly Lys Arg Asn  Met Ile Asp Leu Ser  Ser Ala Gly 
    1325                 1330                 1335             


Gly Ser  Lys Asn Ala Gly Cys  Met Asn Pro Thr Phe  Ser Ser Glu 
    1340                 1345                 1350             


Leu Ser  Ser Pro Gly Ser Asn  Phe Thr Phe Ile Asp  Gln Ser Val 
    1355                 1360                 1365             


Thr Gly  Met Phe Glu Arg Arg  Gln Arg Leu Glu Leu  
    1370                 1375                 1380 


<210>  1097
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1097

Met Val Lys Lys Lys Tyr Ala Ile Gly Ile Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Ile Val Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asn Thr Glu Lys Arg Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Thr Leu Leu Phe Asp Glu Gly Asn Thr Ala Glu Asn Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile 
65                  70                  75                  80  


Leu Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Lys Ile Asp Asp 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Ile Val Glu Asp Lys 
            100                 105                 110         


Gln Gly Ser Lys His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Ser Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Lys 
                165                 170                 175     


Ser Glu Asn Lys Asp Val Gln His Leu Phe Asn Asp Phe Val Glu Met 
            180                 185                 190         


Phe Asp Lys Thr Val Glu Gly Ser Tyr Leu Ser Glu Asn Leu Pro Asn 
        195                 200                 205             


Val Ala Asp Val Leu Val Glu Lys Val Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Ile Leu His Tyr Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly 
225                 230                 235                 240 


Asn Phe Leu Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Glu Leu Ala Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Ile Ala Thr Lys Ser Leu Tyr Asp Gly Ile Leu Leu Ala 
    290                 295                 300                 


Gly Ile Leu Ser Thr Thr Asp Ser Thr Thr Lys Ala Pro Leu Ser Ser 
305                 310                 315                 320 


Ser Met Val Asn Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu 
                325                 330                 335     


Lys Asn Phe Ile His Gln Asn Leu Ser Asp Ser Tyr Lys Glu Val Phe 
            340                 345                 350         


Asn Asp Lys Leu Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Asn Phe Tyr Arg Phe Ile Lys Lys Ala Ile Glu Lys Ile 
    370                 375                 380                 


Glu Gly Ser Asn Tyr Phe Ile Asp Lys Ile Asp Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro 
            420                 425                 430         


Phe Leu Val Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Glu Phe Ala 
    450                 455                 460                 


Trp Leu Asn Arg Lys Ser Asp Glu Lys Ile Arg Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Glu Met Val Asp Lys Glu Thr Ser Ala Glu Asn Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Asn Tyr Asp Arg Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Met Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Asp Ile Val Lys Thr Leu Phe Lys Thr Lys Arg Lys Val 
545                 550                 555                 560 


Thr Ala Lys Asp Ile Lys Ala Tyr Leu Glu Asn Ser Asn Gly Tyr Ala 
                565                 570                 575     


Gly Val Glu Leu Lys Gly Leu Glu Glu Gln Phe Asn Ala Ser Leu Pro 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Leu Arg Asp Lys Ala Phe Ile Asp 
        595                 600                 605             


Ala Glu Glu Asn Gln Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Lys 
625                 630                 635                 640 


Asp Ile Leu Thr Glu Glu Gln Arg Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Leu Asp 
            660                 665                 670         


Lys Val Thr Arg Lys Thr Ile Leu Gly Tyr Leu Ile Asp Asp Gly Thr 
        675                 680                 685             


Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe 
    690                 695                 700                 


Val Asp Glu Ile Arg Leu Ala Gln Gly Ser Gly Glu Ala Glu Asp Tyr 
705                 710                 715                 720 


Arg Ala Glu Val Gln Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Ile Glu Val Met Gly 
            740                 745                 750         


Tyr Glu Pro Glu His Ile Val Val Glu Met Ala Arg Glu Asn Gln Phe 
        755                 760                 765             


Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Ile Asn Arg Leu Glu 
    770                 775                 780                 


Asn Ala Leu Lys Asp Phe Gly Ser Lys Ile Leu Glu Glu Gln Lys Pro 
785                 790                 795                 800 


Tyr Gly Val Ser Lys Val Asn Asn Ile His Leu Gln Ser Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Glu Glu 
            820                 825                 830         


Leu Asp Ile Asp His Leu Ser Glu Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Thr Lys 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Met Glu Ile 
865                 870                 875                 880 


Val His Asn Arg Lys Asn Phe Trp Arg Gln Leu Leu Lys Ser Lys Leu 
                885                 890                 895     


Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Asn Glu Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Met Leu Asp Thr Arg Phe 
    930                 935                 940                 


Asn Thr Lys Leu Asp Glu Ala Gly Asn Arg Ile Arg Asp Pro Lys Val 
945                 950                 955                 960 


Asn Ile Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Tyr Gln Leu Tyr Lys Ile Arg Glu Ile Asn Asn Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Ile Asn Ala Val Val  Ala Thr Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Gln  Leu Ala Pro Glu Phe  Val Tyr Gly Glu Tyr  Pro Thr Tyr 
    1010                 1015                 1020             


Asn Ser  Tyr Asn Ser Arg Lys  Ser Ala Thr Glu Lys  Val Leu Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Arg Ile Val  Val His Ser 
    1040                 1045                 1050             


Lys Thr  Gly Glu Val Arg Ile  Arg Pro Val Ile Glu  Val Asn Lys 
    1055                 1060                 1065             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Lys Ser Asp  Phe Arg Thr 
    1070                 1075                 1080             


Val Arg  Lys Val Leu Ser Tyr  Pro Gln Val Asn Val  Val Lys Lys 
    1085                 1090                 1095             


Thr Glu  Val Gln Asn His Gly  Leu Asp Arg Gly Lys  Pro Lys Gly 
    1100                 1105                 1110             


Phe Tyr  Asn Ala Asn Leu Ser  Pro Arg Pro Lys Glu  Gly Ser Val 
    1115                 1120                 1125             


Glu Asn  Leu Val Pro Ala Lys  Gln Ser Phe Asp Thr  Lys Arg Tyr 
    1130                 1135                 1140             


Gly Gly  Tyr Ala Gly Ile Ser  Asn Ser Tyr Ala Val  Leu Val Asn 
    1145                 1150                 1155             


Gly Ile  Ile Glu Lys Gly Lys  Lys Lys Ser Lys Ala  Glu Val Thr 
    1160                 1165                 1170             


Glu Phe  Gln Gly Ile Ser Ile  Leu Ala Arg Lys Asp  Phe Asp Lys 
    1175                 1180                 1185             


Asn Pro  Lys Gln Tyr Leu Leu  Asn Leu Gly Tyr Lys  Asp Ile Lys 
    1190                 1195                 1200             


Ser Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn 
    1205                 1210                 1215             


Gly Ser  Arg Arg Met Leu Ala  Ser Ile Leu Ser Thr  Asn Asn Lys 
    1220                 1225                 1230             


Arg Gly  Glu Ile His Lys Gly  Asn Glu Met Tyr Leu  Pro Asp Lys 
    1235                 1240                 1245             


Phe Val  Thr Leu Leu Tyr His  Ala Met Arg Val Asn  Arg Thr Leu 
    1250                 1255                 1260             


Glu Pro  Gly His Lys Lys Tyr  Val Glu Thr His Arg  His Val Phe 
    1265                 1270                 1275             


Asp Glu  Leu Leu Ile Tyr Ile  Leu Glu Tyr Asn Glu  Lys Ile Val 
    1280                 1285                 1290             


Gly Ala  Lys Ala Asn Gly Lys  Arg Ile Val Glu Ala  Tyr Ser Ala 
    1295                 1300                 1305             


Arg Lys  Asp Thr Asp Asn Leu  Glu Glu Leu Cys Asn  Ser Phe Ile 
    1310                 1315                 1320             


Asn Leu  Leu Asn Leu Thr Ala  Leu Gly Ser Ala Val  Asp Phe Glu 
    1325                 1330                 1335             


Phe Leu  Gly Thr Lys Ile Pro  Arg Tyr Arg Asp Tyr  Thr Pro Ser 
    1340                 1345                 1350             


Ser Leu  Leu Lys Ala Thr Leu  Ile His Gln Ser Val  Thr Gly Leu 
    1355                 1360                 1365             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu Gly Gly  Asp 
    1370                 1375                 1380     


<210>  1098
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1098

Met Asn Tyr Val Lys Glu Tyr His Ile Gly Leu Asp Ile Gly Thr Ser 
1               5                   10                  15      


Ser Ile Gly Trp Ala Val Thr Asp Ser Gln Phe Lys Leu Met Arg Ile 
            20                  25                  30          


Lys Gly Lys Thr Ala Ile Gly Val Arg Leu Phe Glu Glu Gly Lys Thr 
        35                  40                  45              


Ala Ala Glu Arg Arg Thr Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg 
    50                  55                  60                  


Arg Lys Trp Arg Leu His Tyr Leu Asp Glu Ile Phe Ala Pro His Leu 
65                  70                  75                  80  


Gln Glu Val Asp Glu Asn Phe Leu Arg Arg Leu Lys Gln Ser Asn Ile 
                85                  90                  95      


His Pro Glu Asp Pro Ala Lys Asn Gln Ala Phe Ile Gly Lys Leu Leu 
            100                 105                 110         


Phe Pro Asp Leu Leu Lys Lys Asn Glu Arg Gly Tyr Pro Thr Leu Ile 
        115                 120                 125             


Lys Met Arg Asp Glu Leu Pro Val Glu Gln Arg Ala His Tyr Pro Val 
    130                 135                 140                 


Thr Asn Ile Tyr Lys Leu Arg Glu Ala Met Ile Asn Glu Asp Arg Gln 
145                 150                 155                 160 


Phe Asp Leu Arg Glu Val Tyr Leu Ala Val His His Ile Val Lys Tyr 
                165                 170                 175     


Arg Gly His Phe Leu Asn Asn Ala Ser Val Asp Lys Phe Lys Val Gly 
            180                 185                 190         


Arg Ile Asp Phe Asp Lys Ser Phe Asn Val Leu Asn Glu Ala Tyr Glu 
        195                 200                 205             


Glu Leu Gln Asn Gly Glu Gly Ser Phe Thr Ile Glu Pro Ser Lys Val 
    210                 215                 220                 


Glu Lys Ile Gly Gln Leu Leu Leu Asp Thr Lys Met Arg Lys Leu Asp 
225                 230                 235                 240 


Arg Gln Lys Ala Val Ala Lys Phe Leu Glu Val Lys Val Ala Asp Lys 
                245                 250                 255     


Glu Glu Thr Lys Arg Asn Lys Gln Ile Ala Thr Ala Ile Ser Lys Leu 
            260                 265                 270         


Val Leu Gly Tyr Lys Ala Asp Phe Ala Thr Val Ala Met Ala Asn Gly 
        275                 280                 285             


Asn Glu Trp Lys Ile Asp Leu Ser Ser Glu Thr Ser Glu Asp Glu Ile 
    290                 295                 300                 


Glu Lys Phe Arg Glu Glu Leu Ser Asp Ala Gln Asn Asp Ile Leu Thr 
305                 310                 315                 320 


Glu Ile Thr Ser Leu Phe Ser Gln Ile Met Leu Asn Glu Ile Val Pro 
                325                 330                 335     


Asn Gly Met Ser Ile Ser Glu Ser Met Met Asp Arg Tyr Trp Thr His 
            340                 345                 350         


Glu Arg Gln Leu Ala Glu Val Lys Glu Tyr Leu Ala Thr Gln Pro Ala 
        355                 360                 365             


Ser Ala Arg Lys Glu Phe Asp Gln Val Tyr Asn Lys Tyr Ile Gly Gln 
    370                 375                 380                 


Ala Pro Lys Glu Lys Gly Phe Asp Leu Glu Lys Gly Leu Lys Lys Ile 
385                 390                 395                 400 


Leu Ser Lys Lys Glu Asn Trp Lys Glu Ile Asp Glu Leu Leu Lys Ala 
                405                 410                 415     


Gly Asp Phe Leu Pro Lys Gln Arg Thr Ser Ala Asn Val Val Ile Pro 
            420                 425                 430         


His Gln Met His Gln Gln Glu Leu Asp Arg Ile Ile Glu Lys Gln Ala 
        435                 440                 445             


Lys Tyr Tyr Pro Trp Leu Ala Thr Glu Asn Pro Ala Thr Gly Glu Arg 
    450                 455                 460                 


Asp Arg His Gln Ala Lys Tyr Glu Leu Asp Gln Leu Val Ser Phe Arg 
465                 470                 475                 480 


Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Pro Glu Val Gln Lys Ala 
                485                 490                 495     


Thr Ser Gly Ala Lys Phe Ala Trp Ala Lys Arg Lys Glu Asp Gly Glu 
            500                 505                 510         


Ile Thr Pro Trp Asn Leu Trp Asp Lys Ile Asp Arg Ala Glu Ser Ala 
        515                 520                 525             


Glu Ala Phe Ile Lys Arg Met Thr Val Lys Asp Thr Tyr Leu Leu Asn 
    530                 535                 540                 


Glu Asp Val Leu Pro Ala Asn Ser Leu Leu Tyr Gln Lys Tyr Asn Val 
545                 550                 555                 560 


Leu Asn Glu Leu Asn Asn Val Arg Val Asn Gly Arg Arg Leu Ser Val 
                565                 570                 575     


Gly Ile Lys Gln Asp Ile Tyr Ser Glu Leu Phe Lys Lys Lys Lys Thr 
            580                 585                 590         


Val Lys Ala Ser Asp Val Ala Ser Leu Val Met Ala Lys Thr Arg Gly 
        595                 600                 605             


Val Asn Lys Pro Ser Val Glu Gly Leu Ser Asp Pro Lys Lys Phe Asn 
    610                 615                 620                 


Ser Asn Leu Ala Thr Tyr Leu Asp Leu Lys Ser Ile Val Gly Asp Lys 
625                 630                 635                 640 


Val Asp Asp Asn Cys Tyr Gln Thr Asp Leu Glu Asn Ile Ile Glu Trp 
                645                 650                 655     


Arg Ser Val Phe Glu Asp Gly Glu Ile Phe Ala Asp Lys Leu Thr Glu 
            660                 665                 670         


Val Glu Trp Leu Thr Asp Glu Gln Arg Ser Ala Leu Val Lys Lys Arg 
        675                 680                 685             


Tyr Lys Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gly Ile Val 
    690                 695                 700                 


Asp Glu Asn Gly Gln Arg Ile Ile Asp Leu Met Trp Asn Thr Asp Gln 
705                 710                 715                 720 


Asn Phe Met Gln Ile Val Asn Gln Pro Val Phe Lys Glu Gln Ile Asp 
                725                 730                 735     


Gln Leu Asn Gln Asn Ala Ile Thr Asn Asp Gly Met Thr Leu Arg Glu 
            740                 745                 750         


Arg Val Glu Ser Val Leu Asp Asp Ala Tyr Thr Ser Pro Gln Asn Lys 
        755                 760                 765             


Lys Ala Ile Trp Gln Val Val Arg Val Val Glu Asp Ile Val Lys Ala 
    770                 775                 780                 


Val Gly Asn Ala Pro Lys Ser Ile Ser Ile Glu Phe Ala Arg Asn Glu 
785                 790                 795                 800 


Gly Asn Lys Gly Glu Ile Thr Arg Ser Arg Arg Thr Gln Leu Gln Lys 
                805                 810                 815     


Leu Phe Glu Asp Gln Ala His Glu Leu Val Lys Asp Thr Ser Leu Thr 
            820                 825                 830         


Glu Glu Leu Glu Lys Ala Leu Asp Leu Ser Asp Arg Tyr Tyr Leu Tyr 
        835                 840                 845             


Phe Thr Gln Gly Gly Lys Asp Met Tyr Thr Gly Asp Pro Ile Asn Phe 
    850                 855                 860                 


Asp Glu Ile Ser Thr Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ser 
865                 870                 875                 880 


Phe Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Ala 
                885                 890                 895     


Glu Asn Asn Lys Lys Ser Asp Arg Val Pro Ala Lys Leu Tyr Ala Ala 
            900                 905                 910         


Lys Met Lys Pro Tyr Trp Asn Gln Leu Leu Lys Gln Gly Leu Ile Thr 
        915                 920                 925             


Gln Arg Lys Phe Glu Asn Leu Thr Met Asp Val Asp Gln Asn Ile Lys 
    930                 935                 940                 


Tyr Arg Ser Leu Gly Phe Val Lys Arg Gln Leu Val Glu Thr Arg Gln 
945                 950                 955                 960 


Val Ile Lys Leu Thr Ala Asn Ile Leu Gly Ser Met Tyr Gln Glu Ala 
                965                 970                 975     


Gly Thr Asp Ile Ile Glu Thr Arg Ala Gly Leu Thr Lys Gln Leu Arg 
            980                 985                 990         


Glu Glu Phe Asp Leu Pro Lys Val  Arg Glu Val Asn Asp  Tyr His His 
        995                 1000                 1005             


Ala Val  Asp Ala Tyr Leu Thr  Thr Phe Ala Gly Gln  Tyr Leu Asn 
    1010                 1015                 1020             


Arg Arg  Tyr Pro Lys Leu Arg  Ser Phe Phe Val Tyr  Gly Glu Tyr 
    1025                 1030                 1035             


Met Lys  Phe Lys Arg Gly Ser  Asp Leu Lys Leu Arg  Asn Phe Asn 
    1040                 1045                 1050             


Phe Phe  His Glu Leu Met Glu  Gly Asp Lys Ser Gln  Gly Lys Val 
    1055                 1060                 1065             


Val Asp  Gln Gln Thr Gly Glu  Leu Ile Thr Thr Arg  Asp Glu Val 
    1070                 1075                 1080             


Ala Lys  Ser Phe Asp Arg Leu  Leu Asn Met Lys Tyr  Met Leu Val 
    1085                 1090                 1095             


Ser Lys  Glu Val His Asp Arg  Ser Asp Gln Leu Tyr  Gly Ala Thr 
    1100                 1105                 1110             


Ile Val  Thr Ala Lys Glu Ser  Gly Lys Leu Thr Ser  Pro Ile Glu 
    1115                 1120                 1125             


Ile Lys  Lys Asn Arg Pro Val  Asp Leu Tyr Gly Ala  Tyr Thr Asn 
    1130                 1135                 1140             


Gly Thr  Ser Ala Phe Met Thr  Ile Ile Lys Phe Thr  Gly Asn Lys 
    1145                 1150                 1155             


Pro Lys  Tyr Lys Val Ile Gly  Val Pro Thr Thr Ser  Ala Val Gly 
    1160                 1165                 1170             


Leu Lys  Arg Val Gly Lys Pro  Gly Ser Glu Ser Tyr  Asn Gln Glu 
    1175                 1180                 1185             


Leu His  Arg Ile Ile Lys Ser  Asn Pro Lys Val Lys  Lys Asp Phe 
    1190                 1195                 1200             


Glu Ile  Val Val Pro His Val  Ser Tyr Ser Gln Leu  Ile Val Asp 
    1205                 1210                 1215             


Gly Asp  Cys Lys Phe Thr Leu  Ala Ser Asp Thr Tyr  Gln His Pro 
    1220                 1225                 1230             


Ala Thr  Gln Leu Val Leu Ser  Lys Glu Ser Met Glu  Ile Ile Ala 
    1235                 1240                 1245             


Asp Asp  Phe Lys Phe Val Lys  Glu Asn Pro Ala Thr  Ala Asp Glu 
    1250                 1255                 1260             


Gln Leu  Val Arg Val Phe Asp  Glu Ile Val Asn Gln  Met Asn His 
    1265                 1270                 1275             


Tyr Phe  Thr Ile Phe Asp Gln  Arg Ser Asn Arg Gln  Lys Val Thr 
    1280                 1285                 1290             


Lys Ala  Arg Asp Lys Phe Val  Ser Leu Pro Thr Glu  Ser Glu Tyr 
    1295                 1300                 1305             


Glu Gly  Ala Lys Lys Thr Gln  Val Gly Lys Thr Glu  Val Ile Thr 
    1310                 1315                 1320             


Asn Leu  Leu Met Gly Leu His  Ala Asn Ala Ala Gln  Gly Asp Leu 
    1325                 1330                 1335             


Lys Val  Leu Gly Leu Ser Thr  Phe Gly Phe Phe Gln  Ser Ser Gly 
    1340                 1345                 1350             


Gly Leu  Asn Leu Ser Glu Asp  Ala Met Ile Val Tyr  Gln Ser Pro 
    1355                 1360                 1365             


Thr Gly  Leu Phe Glu Arg Arg  Ile Cys Leu Lys Asp  Ile 
    1370                 1375                 1380     


<210>  1099
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1099

Met Lys Gln Ile Leu Lys Lys Val Gln Gly Tyr Asn Ile Gly Leu Asp 
1               5                   10                  15      


Ile Gly Thr Asn Ser Val Gly Trp Val Val Thr Asp Glu Asn Ala Asn 
            20                  25                  30          


Leu Leu Gln Phe Arg Lys Lys Asn Met Trp Gly Ala Arg Leu Phe Glu 
        35                  40                  45              


Lys Gly Asp Thr Ala Gln Gly Arg Arg Leu Ser Arg Ser Thr Arg Arg 
    50                  55                  60                  


Arg Tyr Asp Arg Arg Arg Gln Arg Ile His Tyr Leu Gln Glu Met Leu 
65                  70                  75                  80  


Ala Thr Met Val Leu Pro Ile Asp Glu Ser Phe Phe Ile Arg Leu Asn 
                85                  90                  95      


Glu Ala Phe Leu Trp Lys Glu Asp Lys Ser Val Gln Arg Asp Tyr Ile 
            100                 105                 110         


Leu Phe Ala Asp Lys Gln Phe Thr Asp Arg Asp Phe Tyr Lys Gln Phe 
        115                 120                 125             


Lys Thr Ile Tyr His Leu Arg Lys Phe Leu Leu Asp Thr Lys Glu Lys 
    130                 135                 140                 


Ala Asp Pro Arg Phe Ile Tyr Leu Ala Leu His His Met Ile Lys Tyr 
145                 150                 155                 160 


Arg Gly Asn Phe Leu Tyr Gln Gly Gln Ser Phe Asp Val Val Glu Asn 
                165                 170                 175     


Val Gln Glu Ser Phe Arg Val Leu Leu Glu Glu Met Tyr Glu Gln Leu 
            180                 185                 190         


Gly Lys Glu Trp Glu Tyr Gly Glu His Phe Asp Asp Ile Gln Arg Ile 
        195                 200                 205             


Leu Glu Asn Thr Thr Ile Gly Lys Lys Ser Lys Gln Glu Gln Ile Ala 
    210                 215                 220                 


Ala Ile Leu Thr Glu Gln Tyr Gly Asn Lys Lys Leu Ala Asn Glu Val 
225                 230                 235                 240 


Thr Arg Ala Cys Leu Gly Tyr Ser Phe Asp Leu Thr Val Val Met Glu 
                245                 250                 255     


Asn Glu Asn Leu Leu Asp Glu Ser Gly Lys Lys Ile Lys Ile Ser Phe 
            260                 265                 270         


Ser Asp Ser Lys Tyr Glu Glu Gln Glu Leu Glu Leu Gln Asn Arg Leu 
        275                 280                 285             


Glu Glu Lys Phe Ala Val Ile Glu Ala Leu Lys Arg Ile Tyr Asn Trp 
    290                 295                 300                 


Leu Val Leu Lys Asp Thr Leu Arg Gly Lys Lys Tyr Ile Ser Glu Ala 
305                 310                 315                 320 


Met Val Asn Lys Tyr Glu Ala His His Glu Asp Leu Val Lys Leu Lys 
                325                 330                 335     


Arg Leu Phe Arg Thr Tyr Leu Ser Thr Glu Glu Tyr Ser Cys Phe Phe 
            340                 345                 350         


Arg Lys Glu Lys Gln Lys Asp Lys Tyr Ile Ser Asn Tyr Val Asn Tyr 
        355                 360                 365             


Arg Lys Gly Ala Glu Arg Cys Ser Lys Glu Lys Leu Tyr Lys Ala Ile 
    370                 375                 380                 


His Glu Leu Phe Asp Thr Val Ala Lys Asp Asp Val Val Tyr Gln Glu 
385                 390                 395                 400 


Ile Val Glu Lys Met Asp Gln Gly Glu Phe Leu Thr Lys Gln Asn Glu 
                405                 410                 415     


Val Ser Asn Ala Tyr Ile Pro Tyr Gln Cys Asn Glu Val Glu Met Lys 
            420                 425                 430         


Arg Ile Leu Lys Gln Gln Gly Glu Phe Tyr Pro Glu Leu Lys Glu Asn 
        435                 440                 445             


Ala Asp Lys Ile Leu Gln Leu Leu Leu Phe Arg Ile Pro Tyr Tyr Val 
    450                 455                 460                 


Gly Pro Leu Val Asp Glu Lys Gln Ser Lys Phe Ala Trp Met Lys Arg 
465                 470                 475                 480 


Arg Thr Gly Met Glu Gln Val Lys Ile Tyr Pro Trp Asn Phe Glu Gln 
                485                 490                 495     


Val Val Asp Ile Asp Gln Thr Ala Glu Ala Phe Ile Thr Arg Met Thr 
            500                 505                 510         


Asn Tyr Cys Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys Gln Ser 
        515                 520                 525             


Leu Leu Tyr Ser Glu Tyr Glu Leu Leu Gln Glu Leu Asn Lys Ile Lys 
    530                 535                 540                 


Ile Asn Gly Lys Phe Ser Leu Glu Thr Arg Asn Lys Ile Ile Glu Gln 
545                 550                 555                 560 


Leu Phe Lys Lys Gln Lys Lys Val Thr Glu Lys Gln Leu Lys Lys Trp 
                565                 570                 575     


Leu Val Arg Glu Gln Tyr Cys Thr Thr Lys Val Thr Glu Lys Met Val 
            580                 585                 590         


Lys Leu Asp Glu Asn Ala Tyr Tyr Ile Thr Gly Phe Gln Lys Glu Asn 
        595                 600                 605             


Glu Phe Ala Ser Ser Leu Thr Ser Tyr Ile Asp Phe Thr Lys Ile Leu 
    610                 615                 620                 


Gly Glu Ile Thr Glu Arg Asn Arg Lys Met Ile Glu Glu Ile Ile Tyr 
625                 630                 635                 640 


Trp Leu Thr Val Phe Glu Glu Lys Ser Ile Val Lys Arg Lys Ile Gln 
                645                 650                 655     


Lys Lys Tyr Gly Asn Glu Ile Asn Asp Lys Gln Leu Ala Gln Ile Leu 
            660                 665                 670         


Lys Leu Asn Tyr Ser Gly Trp Gly Arg Phe Ser Glu Lys Phe Leu Asn 
        675                 680                 685             


Gly Ile Lys Gly Phe Asp Arg Gln Gly Gln Lys Lys Thr Ile Met Glu 
    690                 695                 700                 


Leu Leu Arg Glu Asp Ser Arg Leu Leu Asn Ile Met Gln Ile Ile Asn 
705                 710                 715                 720 


Asn Glu Glu Met Gly Phe Cys Lys Ile Ile Glu Ala Asn Gln Lys Lys 
                725                 730                 735     


Leu Tyr Gly Asn Glu Asn Leu Leu Asp Val Val Gln Glu Leu His Gly 
            740                 745                 750         


Ser Pro Ala Ile Lys Arg Gly Ile Trp Gln Ser Leu Lys Ile Val Glu 
        755                 760                 765             


Glu Ile Ile Ser Ile Met Gly Gly Val Pro Gln Asn Ile Phe Ile Glu 
    770                 775                 780                 


Phe Ala Arg Glu Asp Asp Asp Arg Val Arg Thr Asn Ser Arg Val Lys 
785                 790                 795                 800 


Gln Ile Glu Arg Leu Tyr Lys Lys Phe Lys Glu Glu Val Asn Asp Phe 
                805                 810                 815     


Asn Glu His Ala Ala Asn Glu Leu Lys Glu Lys Arg Tyr Gln Asn Arg 
            820                 825                 830         


Ile Asp Glu Glu Lys Val Phe Leu Tyr Phe Met Gln Asn Gly Lys Ser 
        835                 840                 845             


Leu Tyr Ser Gly Val Thr Leu Asp Ile Asp Arg Leu Asn Glu Tyr Glu 
    850                 855                 860                 


Val Asp His Ile Ile Pro Arg Thr Leu Val Lys Asp Asp Ser Leu Glu 
865                 870                 875                 880 


Asn Lys Ala Leu Val Leu Lys Gly Glu Asn Gln Arg Lys Gly Glu Gly 
                885                 890                 895     


Leu Ile Pro His Glu Trp Gln Asn Arg Gln Lys Glu Val Trp Lys Lys 
            900                 905                 910         


Leu Leu Ser Cys Gly Leu Met Gly Lys Lys Lys Tyr Ala Asn Leu Cys 
        915                 920                 925             


Lys Glu Ser Phe Gly Asn Glu Lys Glu Gln Lys His Phe Ile Ala Arg 
    930                 935                 940                 


Gln Ile Val Glu Thr Arg Gln Ile Ile Lys Gln Val Ala Asn Ile Leu 
945                 950                 955                 960 


Ala Gln Tyr Tyr Gln Gly Val Asn Val Ala Ala Val Lys Ala Ser Leu 
                965                 970                 975     


Gly Ser Glu Leu Arg Glu Asn Tyr Thr Ile Glu Lys Lys Asp Asp Asn 
            980                 985                 990         


Gly Phe Phe Met Glu Asn Lys Ser  Tyr Thr Met Leu Lys  Ser Arg Lys 
        995                 1000                 1005             


Ile Asn  Asp Phe His His Ala  Gln Asp Ala Tyr Leu  Ala Cys Ala 
    1010                 1015                 1020             


Val Gly  Leu Tyr Ile Lys Ala  Ala Phe Pro Lys Tyr  Glu Lys Glu 
    1025                 1030                 1035             


Ile Asp  Tyr Glu Gly Tyr Arg  Lys Tyr Tyr Gln Lys  Lys Asn Gln 
    1040                 1045                 1050             


Gln Lys  Asp Gly Val Arg Asn  Glu Lys Arg Gln Phe  Phe Ser Tyr 
    1055                 1060                 1065             


Val Met  Ser Arg Phe Ser Glu  Gln Ile Val Asp Lys  Glu Thr Gly 
    1070                 1075                 1080             


Glu Ile  Leu Trp Asp Gly Thr  Gln Lys Leu Ala Tyr  Ile Lys Lys 
    1085                 1090                 1095             


Ile Phe  Gly Tyr Arg Asp Cys  Leu Ile Thr Lys Lys  Leu Glu Glu 
    1100                 1105                 1110             


Asn Thr  Gly Glu Phe Tyr Lys  Gln Thr Ile Asn Ala  Lys Pro Glu 
    1115                 1120                 1125             


Lys Gly  Lys Glu Asn Asn Thr  Lys Leu Leu Pro Ile  Lys Lys Gly 
    1130                 1135                 1140             


Leu Ser  Val Glu Lys Tyr Gly  Gly Tyr Gln Gly Gly  Glu Ala Ala 
    1145                 1150                 1155             


Tyr Ser  Cys Leu Val Arg Tyr  Gln Gln Gly Arg Lys  Thr Lys Leu 
    1160                 1165                 1170             


Ser Leu  Glu Lys Ile Ser Val  Ile Ile Ala Lys Lys  Ile Ala Glu 
    1175                 1180                 1185             


Lys Glu  Val Ser Leu Asp Arg  Tyr Leu Lys Glu Lys  Leu Gly Val 
    1190                 1195                 1200             


Glu Glu  Ile Val Ile Leu Arg  Lys Lys Val Met Lys  Asn Gln Leu 
    1205                 1210                 1215             


Ile Glu  Asp Ser Asp Gly Asn  Arg Phe Tyr Leu Thr  Ala Ala Thr 
    1220                 1225                 1230             


Glu Val  Val Asn Ala Lys Gln  Phe Ile Leu Asp Gly  Lys His Gln 
    1235                 1240                 1245             


His Leu  Leu Arg Thr Ile Ala  Ile Met Glu Lys Gly  Glu Tyr Tyr 
    1250                 1255                 1260             


Gly Tyr  Arg Leu Asp Glu Leu  Glu Asn Ser Met Asp  Glu Leu Tyr 
    1265                 1270                 1275             


Ala Tyr  Tyr Arg Met Lys Ile  Gln Gln Gln Tyr Pro  Ala Tyr Glu 
    1280                 1285                 1290             


Ser Ile  Val Lys Lys Ile Val  Glu Ser His Val Tyr  Glu Ser Leu 
    1295                 1300                 1305             


His Val  Glu Glu Lys Ile Lys  Ala Val Gln Glu Lys  Gly Lys Phe 
    1310                 1315                 1320             


Leu Ile  Gln Leu Leu Arg Leu  Thr Glu Ala Asn Ala  Glu Cys Ile 
    1325                 1330                 1335             


Asp Leu  Lys Thr Ile Ser Asp  Gly Arg Leu Ala Asp  Arg Ile Gly 
    1340                 1345                 1350             


Arg Lys  Asn His Tyr Thr Ile  Lys Glu Gly Ile Lys  Phe Ile Asp 
    1355                 1360                 1365             


Gln Ser  Ile Thr Gly Leu Tyr  Glu Arg Tyr Met Thr  Val 
    1370                 1375                 1380     


<210>  1100
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1100

Met Asp Tyr Val Lys Glu Tyr His Ile Gly Leu Asp Ile Gly Thr Ser 
1               5                   10                  15      


Ser Ile Gly Trp Ala Val Thr Asp Ser Gln Phe Lys Leu Met Arg Ile 
            20                  25                  30          


Lys Gly Lys Thr Ala Ile Gly Val Arg Leu Phe Glu Glu Gly Lys Thr 
        35                  40                  45              


Ala Ala Glu Arg Arg Thr Phe Arg Thr Thr Arg Arg Arg Leu Lys Arg 
    50                  55                  60                  


Arg Lys Trp Arg Leu His Tyr Leu Asp Lys Ile Phe Ala Pro His Leu 
65                  70                  75                  80  


Gln Glu Val Asp Glu Asn Phe Leu Arg Arg Leu Lys Gln Ser Asn Ile 
                85                  90                  95      


His Pro Glu Asp Pro Ala Lys Asn Gln Ala Phe Ile Gly Lys Leu Leu 
            100                 105                 110         


Phe Pro Asp Leu Leu Lys Lys Asn Glu Arg Gly Tyr Pro Thr Leu Ile 
        115                 120                 125             


Lys Met Arg Asp Glu Leu Pro Val Glu Gln Arg Ala His Tyr Pro Val 
    130                 135                 140                 


Thr Asn Ile Tyr Lys Leu Arg Glu Ala Met Ile Asn Glu Asp Arg Gln 
145                 150                 155                 160 


Phe Asp Leu Arg Glu Val Tyr Leu Ala Val His His Ile Val Lys Tyr 
                165                 170                 175     


Arg Gly His Phe Leu Asn Asn Ala Ser Val Asp Lys Phe Lys Val Gly 
            180                 185                 190         


Arg Ile Asp Phe Asp Lys Ser Phe Asn Val Leu Asn Glu Ala Tyr Glu 
        195                 200                 205             


Glu Leu Gln Asn Gly Glu Gly Ser Phe Thr Ile Glu Pro Ser Lys Val 
    210                 215                 220                 


Glu Lys Ile Gly Gln Leu Leu Leu Asp Thr Lys Met Arg Lys Leu Asp 
225                 230                 235                 240 


Arg Gln Lys Ala Val Ala Lys Leu Leu Glu Val Lys Val Ala Asp Lys 
                245                 250                 255     


Glu Glu Thr Lys Arg Asn Lys Gln Ile Ala Thr Ala Met Ser Lys Leu 
            260                 265                 270         


Val Leu Gly Tyr Lys Ala Asp Phe Ala Thr Val Ala Met Ala Asn Gly 
        275                 280                 285             


Asn Glu Trp Lys Ile Asp Leu Ser Ser Glu Thr Ser Glu Asp Glu Ile 
    290                 295                 300                 


Glu Lys Phe Arg Glu Glu Leu Ser Asp Ala Gln Asn Asp Ile Leu Thr 
305                 310                 315                 320 


Glu Ile Thr Ser Leu Phe Ser Gln Ile Met Leu Asn Ala Ile Val Pro 
                325                 330                 335     


Asn Gly Met Ser Ile Ser Glu Ser Met Met Asp Arg Tyr Trp Thr His 
            340                 345                 350         


Glu Arg Gln Leu Ala Glu Val Lys Glu Tyr Leu Ala Thr Gln Pro Ala 
        355                 360                 365             


Ser Ala Arg Lys Glu Phe Asp Gln Val Tyr Asn Lys Tyr Ile Gly Gln 
    370                 375                 380                 


Val Pro Lys Glu Lys Gly Phe Asp Leu Glu Lys Gly Leu Lys Lys Ile 
385                 390                 395                 400 


Leu Ser Lys Lys Glu Asn Trp Lys Glu Ile Asp Glu Leu Leu Lys Ala 
                405                 410                 415     


Gly Asp Phe Leu Pro Lys Gln Arg Thr Ser Ala Asn Gly Val Ile Pro 
            420                 425                 430         


His Gln Met His Gln Gln Glu Leu Asp Arg Ile Ile Glu Lys Gln Ala 
        435                 440                 445             


Lys Tyr Tyr Pro Trp Leu Ala Thr Glu Asn Pro Ala Thr Gly Glu Arg 
    450                 455                 460                 


Asp Arg His Gln Ala Lys Tyr Glu Leu Asp Gln Leu Val Ser Phe Arg 
465                 470                 475                 480 


Ile Pro Tyr Tyr Val Gly Pro Leu Val Thr Pro Glu Val Gln Lys Ala 
                485                 490                 495     


Thr Ser Gly Ala Lys Phe Ala Trp Ala Lys Arg Lys Glu Asp Gly Glu 
            500                 505                 510         


Ile Thr Pro Trp Asn Leu Trp Asp Lys Ile Asp Arg Ala Glu Ser Ala 
        515                 520                 525             


Glu Ala Phe Ile Lys Arg Met Thr Val Lys Asp Thr Tyr Leu Leu Asn 
    530                 535                 540                 


Glu Asp Val Leu Pro Ala Asn Ser Leu Leu Tyr Gln Lys Tyr Asn Val 
545                 550                 555                 560 


Leu Asn Glu Leu Asn Asn Val Arg Val Asn Gly Arg Arg Leu Ser Val 
                565                 570                 575     


Gly Ile Lys Gln Asp Ile Tyr Thr Glu Leu Phe Lys Lys Lys Lys Thr 
            580                 585                 590         


Val Lys Ala Gly Asp Val Ala Ser Leu Val Met Ala Lys Thr Arg Gly 
        595                 600                 605             


Val Asn Lys Pro Ser Val Glu Gly Leu Ser Asp Pro Lys Lys Phe Asn 
    610                 615                 620                 


Ser Asn Leu Ala Thr Tyr Leu Asp Leu Lys Ser Ile Met Gly Asp Lys 
625                 630                 635                 640 


Val Asp Asp Asn Arg Tyr Gln Met Asp Leu Glu Asn Ile Ile Glu Trp 
                645                 650                 655     


Arg Ser Val Phe Glu Asp Gly Glu Ile Phe Ala Asp Lys Leu Thr Glu 
            660                 665                 670         


Val Glu Trp Leu Thr Asp Glu Gln Arg Ser Ala Leu Val Lys Lys Arg 
        675                 680                 685             


Tyr Lys Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gly Ile Val 
    690                 695                 700                 


Asp Glu Asn Gly Gln Arg Ile Ile Asp Leu Met Trp Asn Thr Asp Gln 
705                 710                 715                 720 


Asn Phe Met Gln Ile Val Asn Gln Pro Val Phe Lys Glu Gln Ile Asp 
                725                 730                 735     


Gln Leu Asn Gln Lys Ala Ile Thr Asn Asp Gly Met Thr Leu Arg Glu 
            740                 745                 750         


Arg Val Glu Ser Val Leu Asp Asp Ala Tyr Thr Ser Pro Gln Asn Lys 
        755                 760                 765             


Lys Ala Ile Trp Gln Val Val Arg Val Val Glu Asp Ile Val Lys Ala 
    770                 775                 780                 


Val Gly Asn Ala Pro Lys Ser Ile Ser Ile Glu Phe Ala Arg Asn Glu 
785                 790                 795                 800 


Gly Asn Lys Gly Glu Ile Thr Arg Ser Arg Arg Thr Gln Leu Gln Lys 
                805                 810                 815     


Leu Phe Glu Asp Gln Ala His Glu Leu Val Lys Asp Thr Ser Leu Thr 
            820                 825                 830         


Glu Glu Leu Glu Lys Ala Pro Asp Leu Ser Asp Arg Tyr Tyr Phe Tyr 
        835                 840                 845             


Phe Thr Gln Gly Gly Lys Asp Met Tyr Thr Gly Asp Pro Ile Asn Phe 
    850                 855                 860                 


Asp Glu Ile Ser Thr Lys Tyr Asp Ile Asp His Ile Leu Pro Gln Ser 
865                 870                 875                 880 


Phe Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Ala 
                885                 890                 895     


Glu Asn Asn Lys Lys Ser Asp Arg Val Pro Ala Lys Leu Tyr Ala Ala 
            900                 905                 910         


Lys Met Lys Pro Tyr Trp Asn Gln Leu Leu Lys Gln Gly Leu Ile Thr 
        915                 920                 925             


Gln Arg Lys Phe Glu Asn Leu Thr Met Asp Val Asp Gln Asn Ile Lys 
    930                 935                 940                 


Tyr Arg Ser Leu Gly Phe Val Lys Arg Gln Leu Val Glu Thr Arg Gln 
945                 950                 955                 960 


Val Ile Lys Leu Thr Ala Asn Ile Leu Gly Ser Met Tyr Gln Glu Ala 
                965                 970                 975     


Gly Thr Asp Ile Ile Glu Thr Arg Ala Gly Leu Thr Lys Gln Leu Arg 
            980                 985                 990         


Glu Glu Phe Asp Leu Pro Lys Val  Arg Glu Val Asn Asp  Tyr His His 
        995                 1000                 1005             


Ala Val  Asp Ala Tyr Leu Thr  Thr Phe Ala Gly Gln  Tyr Leu Asn 
    1010                 1015                 1020             


Arg Arg  Tyr Pro Lys Leu Arg  Ser Phe Phe Val Tyr  Gly Glu Tyr 
    1025                 1030                 1035             


Met Lys  Phe Lys His Gly Ser  Asp Leu Lys Leu Arg  Asn Phe Asn 
    1040                 1045                 1050             


Phe Phe  His Glu Leu Met Glu  Gly Asp Lys Ser Gln  Gly Lys Val 
    1055                 1060                 1065             


Val Asp  Gln Gln Thr Gly Glu  Leu Ile Thr Thr Arg  Asp Glu Val 
    1070                 1075                 1080             


Ala Lys  Ser Phe Asp Arg Leu  Leu Asn Met Lys Tyr  Met Leu Val 
    1085                 1090                 1095             


Ser Lys  Glu Val His Asp Arg  Ser Asp Gln Leu Tyr  Gly Ala Thr 
    1100                 1105                 1110             


Ile Val  Thr Ala Lys Glu Ser  Gly Lys Leu Thr Ser  Pro Ile Glu 
    1115                 1120                 1125             


Ile Lys  Lys Asn Arg Pro Val  Asp Leu Tyr Gly Ala  Tyr Thr Asn 
    1130                 1135                 1140             


Gly Thr  Ser Ala Phe Met Thr  Ile Ile Lys Phe Thr  Gly Asn Lys 
    1145                 1150                 1155             


Pro Lys  Tyr Lys Val Ile Gly  Val Pro Thr Thr Ser  Ala Val Gly 
    1160                 1165                 1170             


Leu Lys  Arg Val Gly Lys Pro  Gly Ser Glu Ser Tyr  Asn Gln Glu 
    1175                 1180                 1185             


Leu His  Arg Ile Ile Lys Ser  Asn Pro Lys Val Lys  Lys Asp Phe 
    1190                 1195                 1200             


Glu Ile  Val Val Pro His Val  Ser Tyr Ser Gln Leu  Ile Val Asp 
    1205                 1210                 1215             


Gly Asp  Cys Lys Phe Thr Leu  Ala Ser Asp Thr Tyr  Gln His Pro 
    1220                 1225                 1230             


Ala Thr  Gln Leu Val Leu Ser  Lys Glu Ser Met Glu  Ile Ile Ala 
    1235                 1240                 1245             


Asp Asp  Phe Lys Phe Val Lys  Glu Asn Pro Ala Thr  Ala Asp Glu 
    1250                 1255                 1260             


Gln Leu  Val Arg Val Phe Asp  Glu Ile Val Asn Gln  Met Asn His 
    1265                 1270                 1275             


Tyr Phe  Thr Ile Phe Asp Gln  Arg Ser Asn Arg Gln  Lys Val Thr 
    1280                 1285                 1290             


Lys Ala  Arg Asp Lys Phe Val  Ser Leu Pro Thr Glu  Ser Glu Tyr 
    1295                 1300                 1305             


Glu Gly  Ala Lys Lys Thr Gln  Val Gly Lys Thr Glu  Val Ile Thr 
    1310                 1315                 1320             


Asn Leu  Leu Met Gly Leu His  Ala Asn Ala Ala Gln  Gly Asp Leu 
    1325                 1330                 1335             


Lys Val  Leu Gly Leu Ser Thr  Phe Gly Phe Phe Gln  Ser Ser Gly 
    1340                 1345                 1350             


Gly Leu  Asn Leu Ser Glu Asp  Ala Met Ile Val Tyr  Gln Ser Pro 
    1355                 1360                 1365             


Thr Gly  Leu Phe Glu Arg Arg  Ile Cys Leu Lys Asp  Ile 
    1370                 1375                 1380     


<210>  1101
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1101

Met Lys Lys Ser Tyr Ala Ile Gly Ile Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly His Cys Val Met Tyr Thr Glu Asn Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asn Thr Glu Lys Arg Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Thr Leu Leu Phe Asp Glu Gly Asn Thr Ala Glu Asn Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile 
65                  70                  75                  80  


Leu Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Lys Ile Asp Asp 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Ile Val Glu Asp Lys 
            100                 105                 110         


Gln Gly Ser Lys His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Ser Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Lys 
                165                 170                 175     


Ser Glu Asn Lys Asp Val Gln His Leu Phe Asn Asp Phe Val Glu Met 
            180                 185                 190         


Phe Asp Lys Thr Val Glu Gly Ser Tyr Leu Ser Glu Asn Leu Pro Asn 
        195                 200                 205             


Val Ala Asp Val Leu Val Glu Lys Val Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Ile Leu His Tyr Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly 
225                 230                 235                 240 


Asn Phe Leu Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Glu Leu Ala Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Ile Ala Thr Lys Ser Leu Tyr Asp Gly Ile Leu Leu Ala 
    290                 295                 300                 


Gly Ile Leu Ser Thr Thr Asp Ser Thr Thr Lys Ala Pro Leu Ser Ser 
305                 310                 315                 320 


Ser Met Val Asn Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu 
                325                 330                 335     


Lys Asp Phe Ile Arg Gln Asn Leu Ser Asp Ser Tyr Lys Glu Phe Phe 
            340                 345                 350         


Asn Asp Thr Leu Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Asn Phe Tyr Arg Phe Ile Lys Lys Ala Ile Glu Lys Ile 
    370                 375                 380                 


Glu Gly Ser Asn Tyr Phe Ile Asp Lys Ile Asp Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro 
            420                 425                 430         


Phe Leu Val Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Glu Phe Ala 
    450                 455                 460                 


Trp Leu Lys Arg Lys Ser Asp Glu Lys Ile Arg Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Glu Met Val Asp Lys Glu Thr Ser Ala Glu Asn Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Asn Tyr Asp Gln Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Val Thr Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr 
545                 550                 555                 560 


Lys Glu Lys Leu Met Asp Phe Leu Gly Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Leu Met Gly Leu Asp Lys Asp Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Val Ser Lys Asp Leu 
        595                 600                 605             


Leu Asp Asn Pro Glu Asn Glu Asp Ile Leu Glu Asn Val Val Leu Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys 
625                 630                 635                 640 


Tyr Lys Asp Val Leu Thr Glu Glu Gln Arg Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asn Lys Thr Thr Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Thr Ser Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Thr Leu 
    690                 695                 700                 


Ser Phe Val Asp Glu Ile Arg Leu Ala Gln Gly Ser Gly Glu Ala Glu 
705                 710                 715                 720 


Asp Tyr Arg Thr Glu Val Gln Asn Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Ile Gly Val 
            740                 745                 750         


Met Gly Tyr Asn Pro Glu His Val Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Phe Thr Asn Gln Gly Arg Arg Ser Ser Gln Gln Arg Tyr Lys Lys 
    770                 775                 780                 


Ile Glu Asn Ala Ile Lys Asn Leu Asp Ser Asn Leu Asn Ser Lys Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asn Asn Gln Ala Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Glu Glu 
            820                 825                 830         


Leu Asp Ile Asp Gln Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Thr Lys 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val His Lys Lys Lys Asn Phe Trp Lys Gln Leu Leu Asp Ser Gln Leu 
                885                 890                 895     


Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Asn Glu Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Thr Arg Phe 
    930                 935                 940                 


Asn Thr Lys Leu Asp Asp Ala Gly Asn Arg Ile Arg Asp Pro Lys Val 
945                 950                 955                 960 


Asn Ile Ile Thr Leu Lys Ser Ser Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Tyr Gln Leu Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Thr Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Gln  Leu Ala Pro Glu Phe  Val Tyr Gly Asp Tyr  Pro Lys Tyr 
    1010                 1015                 1020             


Asn Ser  Tyr Lys Ser Arg Lys  Ser Ala Thr Glu Arg  Val Leu Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Arg Val Val  Lys Ser Ser 
    1040                 1045                 1050             


Lys Thr  Gly Thr Val Lys Ile  Arg Pro Ile Ile Glu  Val Asn Lys 
    1055                 1060                 1065             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Lys Ser Asp  Phe Arg Thr 
    1070                 1075                 1080             


Val Arg  Lys Val Leu Ser Tyr  Pro Gln Val Asn Val  Val Lys Lys 
    1085                 1090                 1095             


Thr Glu  Val Gln Asn His Gly  Leu Asp Arg Gly Lys  Pro Lys Gly 
    1100                 1105                 1110             


Phe Tyr  Asn Ala Asn Leu Ser  Pro Arg Pro Lys Glu  Gly Ser Val 
    1115                 1120                 1125             


Glu Asn  Leu Val Pro Ala Lys  Gln Ser Phe Asp Thr  Lys Arg Tyr 
    1130                 1135                 1140             


Gly Gly  Tyr Ala Gly Ile Ser  Asn Ser Tyr Ala Val  Leu Val Asn 
    1145                 1150                 1155             


Gly Ile  Ile Glu Lys Gly Lys  Lys Lys Ser Lys Ala  Glu Val Thr 
    1160                 1165                 1170             


Glu Phe  Gln Gly Ile Ser Ile  Leu Ala Arg Lys Asp  Phe Glu Lys 
    1175                 1180                 1185             


Asn Pro  Lys Gln Tyr Leu Leu  Asn Leu Gly Tyr Lys  Asp Ile Lys 
    1190                 1195                 1200             


Ser Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn 
    1205                 1210                 1215             


Gly Ser  Arg Arg Met Leu Ala  Ser Ile Leu Ser Thr  Asn Asn Lys 
    1220                 1225                 1230             


Arg Gly  Glu Ile His Lys Gly  Asn Glu Met Tyr Leu  Pro Asp Lys 
    1235                 1240                 1245             


Phe Val  Thr Leu Leu Tyr His  Ala Met Arg Val Asn  Arg Thr Leu 
    1250                 1255                 1260             


Glu Pro  Gly His Lys Lys Tyr  Val Glu Thr His Arg  His Val Phe 
    1265                 1270                 1275             


Asp Glu  Leu Leu Thr Tyr Ile  Leu Glu Tyr Asn Glu  Lys Ile Val 
    1280                 1285                 1290             


Gly Ala  Lys Ala Asn Gly Lys  Arg Ile Val Glu Ala  Tyr Ser Ala 
    1295                 1300                 1305             


Arg Lys  Asp Thr Asp Asn Leu  Glu Glu Leu Cys Asn  Ser Phe Ile 
    1310                 1315                 1320             


Asn Leu  Leu Asn Leu Thr Ala  Leu Gly Ser Ala Val  Asp Phe Glu 
    1325                 1330                 1335             


Phe Leu  Gly Thr Lys Ile Pro  Arg Tyr Arg Asp Tyr  Thr Pro Ser 
    1340                 1345                 1350             


Ser Leu  Leu Lys Ala Thr Leu  Ile His Gln Ser Val  Thr Gly Leu 
    1355                 1360                 1365             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu Gly Gly  Asp 
    1370                 1375                 1380     


<210>  1102
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1102

Met Lys Lys Ile Ile Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Tyr Val His Glu Ala Glu Ser Ala Gln Glu Arg Ser Glu Ile Val 
            20                  25                  30          


Arg Leu Gly Ala Arg Val Val Pro Leu Thr Thr Asp Glu Gln Asp Glu 
        35                  40                  45              


Phe Lys Lys Gly Lys Ser Ile Thr Thr Asn Ala Ala Arg Leu Ser Lys 
    50                  55                  60                  


Arg Ser Leu Arg Arg Asn Ala Asp Arg Phe Gln Leu Arg Arg Asp Glu 
65                  70                  75                  80  


Leu Ala Gly Val Met Lys Glu Ala Gly Trp Ile Asp Glu Thr Thr Val 
                85                  90                  95      


Leu Ala Glu Gln Gly Pro Arg Ser Thr Phe Glu Thr Leu Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Ser Glu Glu Val Thr Leu Ala Glu Phe Ala Arg Ile 
        115                 120                 125             


Leu Leu Gln Ile Asn Arg Lys Arg Gly Tyr Lys Ser Asn Arg Lys Thr 
    130                 135                 140                 


Asp Gly Asp Glu Glu Gly Ala Leu Ile Asp Gly Met Asp Val Ser Lys 
145                 150                 155                 160 


Gln Leu Asn Ala Ser Gly Gln Thr Pro Gly Glu Trp Val Leu Ala Arg 
                165                 170                 175     


Met Gln Glu Gly Asn Ala Val Ile Pro Ala Phe Tyr Cys Ser Asp Leu 
            180                 185                 190         


Ser Glu Glu Leu Asp Arg Ile Trp Arg Lys Gln Gln Ala Ser His Gln 
        195                 200                 205             


Ala Trp Met Thr Glu Glu Leu His Thr Val Leu Arg His Ala Asp Gly 
    210                 215                 220                 


Arg Ser Ala Ile Arg Leu Met Asp Lys Ala Gly Val Val Pro Asp Ser 
225                 230                 235                 240 


Val Asn Val Lys Glu Arg Arg Arg Val Met Tyr Glu Trp Arg Ala Ala 
                245                 250                 255     


Ala Val Arg Asp Cys Leu Pro Ala Gly Arg Val Ala Ala Ala Leu Met 
            260                 265                 270         


Gln Val Ser Lys Glu Ile Gly Arg Ala Asp Ser Tyr Leu Gly Arg Ile 
        275                 280                 285             


Gly Asp Arg Ser Lys Glu Leu Tyr Phe His Arg Gln Thr Val Gly Gln 
    290                 295                 300                 


Tyr Leu Trp Arg Leu Ile Cys Asp Asn Pro Asn Gln Ser Leu Lys Asn 
305                 310                 315                 320 


Leu Val Phe Tyr Arg Gln Asp Tyr Gln His Glu Phe Glu Thr Leu Trp 
                325                 330                 335     


Ser Ile Gln Thr Lys Tyr His Val Glu Leu Thr Glu Asp Leu Arg Lys 
            340                 345                 350         


Arg Val Gly Arg Ile Ile Phe Phe Gln Arg Pro Leu Lys Ser Lys Lys 
        355                 360                 365             


Gly Leu Val Asn Arg Cys Glu Leu Val His Tyr Ser Val Cys Glu Asn 
    370                 375                 380                 


Ser Gly Asp Asp Leu Thr Glu Lys Val Asp Val Gly Pro Lys Val Cys 
385                 390                 395                 400 


Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Val Trp Gln Val Leu 
                405                 410                 415     


Asn Asn Val Glu Val Ile His Pro Ser Thr Gln Glu Ala Arg Arg Leu 
            420                 425                 430         


Glu Met Glu Glu Lys Glu Leu Leu Ala Ser Glu Leu Leu Phe Arg Asp 
        435                 440                 445             


Arg Met Lys Lys Ile Asp Val Leu Lys Leu Leu Phe Ser Asn Trp Arg 
    450                 455                 460                 


Asp Leu Asp Leu Asn Tyr Lys Glu Leu Glu Gly Asn Arg Thr Met Ala 
465                 470                 475                 480 


Ala Phe Tyr Arg Val Tyr Glu Gln Val Ile Ala Gln Thr Gly His Gly 
                485                 490                 495     


Asp Tyr Asp Phe Ala Lys Met Arg Ala Ala Lys Val Lys Glu Leu Ala 
            500                 505                 510         


Glu Gly Val Phe Ala Gly Leu Gly Phe Glu Val Gly Leu Leu Thr Phe 
        515                 520                 525             


Asp Ala Glu Leu Asp Ser Pro Ala Tyr Glu Gln Gln Pro Cys Tyr Arg 
    530                 535                 540                 


Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Lys Ser Ala Thr Gly 
545                 550                 555                 560 


Asn Lys Cys Leu Leu Lys Lys Leu Cys Arg Leu Thr Gly Met Asn Ala 
                565                 570                 575     


Glu Leu Val Gly Leu Phe Met Gln Ile Arg Phe Ser Gly Asp Tyr Gly 
            580                 585                 590         


Ser Leu Ser Thr Lys Ala Met Arg Arg Ile Leu Pro Tyr Leu Lys Ala 
        595                 600                 605             


Gly Asn Thr Tyr Asp Val Ala Cys Glu Tyr Ala Gly Phe Arg His Ser 
    610                 615                 620                 


Ala Arg Ser Leu Thr Arg Glu Glu Leu Ala Gln Lys Glu Tyr Lys Asp 
625                 630                 635                 640 


Tyr Leu Asp Leu Leu Pro Arg Asn Ser Leu Arg Asn Pro Val Val Glu 
                645                 650                 655     


Lys Ile Leu Asn Gln Met Ile His Val Val Asn Gly Leu Ser Ser Thr 
            660                 665                 670         


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Met Ala Arg Glu Leu Lys 
        675                 680                 685             


Lys Ser Ala Arg Glu Arg Glu Glu Met Thr Ser Ala Ile Ser Lys Thr 
    690                 695                 700                 


Thr Lys Glu Asn Ala Glu Ile Thr Ala Ile Leu Gln Arg Asp Phe Gly 
705                 710                 715                 720 


Leu Arg Tyr Val Ser Arg Asn Asp Ile Leu Arg Tyr Lys Leu Tyr Cys 
                725                 730                 735     


Glu Leu Arg Ile Lys Gly Asn Gly Cys Arg Thr Leu Tyr Ser Asn Thr 
            740                 745                 750         


Tyr Ile Asn Pro Asn Glu Leu Phe Ser Arg Glu Phe Asp Ile Glu His 
        755                 760                 765             


Ile Ile Pro Gln Ser Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr 
    770                 775                 780                 


Leu Glu Ser Arg Thr Ala Asn Leu Glu Lys Gly Asp Arg Thr Ala Trp 
785                 790                 795                 800 


Asp Tyr Val Asn Glu Lys Tyr Gly Ala Glu Arg Ala Asp Glu Tyr Arg 
                805                 810                 815     


Ala Gln Val Glu Ala Leu Tyr Arg Asp Gly Thr Ile Ser Lys Ala Lys 
            820                 825                 830         


Arg Asp Lys Leu Leu Met Pro Gly Asp Lys Ile Pro Glu Asp Phe Ile 
        835                 840                 845             


Asp Arg Asp Ile Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala Leu Glu 
    850                 855                 860                 


Ile Phe Glu Asp Phe Val Pro Ser Val Val Ala Thr Ser Gly Asn Val 
865                 870                 875                 880 


Thr Ala Arg Leu Arg Glu Asp Trp Gly Leu Val Asp Val Met Lys Glu 
                885                 890                 895     


Leu Asn Trp Ser Lys Tyr Glu Ser Gln Gly Met Val His Ile Glu Tyr 
            900                 905                 910         


Asp Arg Asp Gly Arg Pro Ile Arg Arg Ile Val Asp Trp Thr Lys Arg 
        915                 920                 925             


Asn Asp His Arg His His Ala Met Asp Ala Leu Ala Val Ala Phe Thr 
    930                 935                 940                 


Lys Arg Ser Tyr Ile Gln Tyr Leu Asn His Leu Asn Ala Arg Lys Glu 
945                 950                 955                 960 


Ala Thr Ser Val Ala Tyr Ala Ile Gln His Lys Glu Met Glu Leu Asp 
                965                 970                 975     


Gly Ser Asn Arg Trp Arg Phe Lys Cys Pro Phe Gly Asp Arg Ser Ala 
            980                 985                 990         


Phe Arg Arg Glu Ala Lys Ala Gln  Leu Glu Arg Ile Leu  Val Ser Ile 
        995                 1000                 1005             


Lys Ser  Lys Asn Lys Val Leu  Thr Pro Ser Val Asn  Arg Thr Lys 
    1010                 1015                 1020             


Lys Arg  Gly Gly Phe Asn Lys  Lys Val Glu Trp Thr  Pro Arg Gly 
    1025                 1030                 1035             


Ala Leu  His Asn Glu Thr Val  Tyr Gly Arg Ile Arg  Arg Tyr Asp 
    1040                 1045                 1050             


Val Lys  Glu Leu Arg Val Gly  Ser Ser Phe Thr Ala  Glu Val Ile 
    1055                 1060                 1065             


Gly Lys  Val Ala Asp Ser Arg  Tyr Arg Ala Ala Leu  Met His Arg 
    1070                 1075                 1080             


Leu Ala  Glu Phe Gly Gly Asp  Pro Lys Lys Ala Phe  Thr Gly Lys 
    1085                 1090                 1095             


Asn Ser  Leu Glu Lys Asn Pro  Leu Tyr Thr Asp Glu  Ala His Thr 
    1100                 1105                 1110             


Ala Cys  Val Pro Ser Ser Val  Lys Val Val Ser Phe  Val Glu Ile 
    1115                 1120                 1125             


Gly Thr  Ile Arg Lys Gly Val  Asp Pro Asn Leu Asn  Val Asp Lys 
    1130                 1135                 1140             


Val Val  Asp Thr His Ile Lys  Asn Ile Leu Leu Ala  Arg Leu Ser 
    1145                 1150                 1155             


Ala Tyr  Gly Gly Asp Ala Lys  Lys Ala Phe Ser Asn  Leu Asp Ala 
    1160                 1165                 1170             


Asp Pro  Ile Trp Leu Asn Lys  Glu Lys Gly Ile Ala  Ile Lys Arg 
    1175                 1180                 1185             


Val Thr  Ile Ser Asp Asp Ser  Glu Val Arg Pro Ile  His Val Lys 
    1190                 1195                 1200             


Arg Asp  Met Arg Gly Arg Leu  Val Cys Asp Glu His  Gly Arg Pro 
    1205                 1210                 1215             


Val Pro  Ser Asp Tyr Val Gln  Leu Arg Asn Asn His  His Val Ala 
    1220                 1225                 1230             


Ile Phe  Arg Asp Ala Asn Gly  Glu Leu Lys Glu Arg  Val Val Thr 
    1235                 1240                 1245             


Phe Phe  Glu Ala Val Glu Arg  Val Arg Gln Leu Arg  Ser Glu Gly 
    1250                 1255                 1260             


Arg Ala  Val Glu Val Ile Asp  Lys Asn Tyr Lys Cys  Glu Glu Gly 
    1265                 1270                 1275             


Trp Thr  Phe Leu Phe Ser Met  Lys Ile Asn Glu Tyr  Phe Val Phe 
    1280                 1285                 1290             


Pro Asp  Ala Ala Ser Gly Phe  Asp Pro Arg Glu Ile  Asp Leu Thr 
    1295                 1300                 1305             


Asp Pro  Ala Asn Tyr Ala Leu  Ile Ser Pro His Leu  Phe Arg Val 
    1310                 1315                 1320             


Gln Lys  Leu Ser Ser Lys Asp  Tyr Tyr Phe Arg His  His Leu Glu 
    1325                 1330                 1335             


Thr Thr  Val Asn Asp Val Lys  Gln Leu Arg Asp Ile  Thr Trp Lys 
    1340                 1345                 1350             


Arg Ile  Ser Ser Leu Gln Asp  Leu Ala Gly Thr Val  Lys Val Arg 
    1355                 1360                 1365             


Val Asn  His Ile Gly Gln Ile  Val Ala Val Gly Glu  Tyr 
    1370                 1375                 1380     


<210>  1103
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1103

Met Val Lys Lys Lys Tyr Ala Ile Gly Ile Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Ile Val Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asn Thr Glu Lys Arg Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Thr Leu Leu Phe Asp Glu Gly Asn Thr Ala Glu Asn Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile 
65                  70                  75                  80  


Leu Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Lys Ile Asp Asp 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Ile Val Glu Asp Lys 
            100                 105                 110         


Gln Gly Ser Lys His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Ser Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Lys 
                165                 170                 175     


Ser Glu Asn Lys Asp Val Gln His Leu Phe Asn Asp Phe Val Glu Met 
            180                 185                 190         


Phe Asp Lys Thr Val Glu Gly Ser Tyr Leu Ser Glu Asn Leu Pro Asn 
        195                 200                 205             


Val Ala Asp Val Leu Val Glu Lys Val Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Ile Leu His Tyr Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly 
225                 230                 235                 240 


Asn Phe Leu Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Glu Leu Ala Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Ile Ala Thr Lys Ser Leu Tyr Asp Gly Ile Leu Leu Ala 
    290                 295                 300                 


Gly Ile Leu Ser Thr Thr Asp Ser Thr Thr Lys Ala Pro Leu Ser Ser 
305                 310                 315                 320 


Ser Met Val Asn Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu 
                325                 330                 335     


Lys Asn Phe Ile His Gln Asn Leu Ser Asp Ser Tyr Lys Glu Val Phe 
            340                 345                 350         


Asn Asp Lys Leu Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Asn Phe Tyr Arg Phe Ile Lys Lys Ala Ile Glu Lys Ile 
    370                 375                 380                 


Glu Gly Ser Asn Tyr Phe Ile Asp Lys Ile Asp Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met His Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro 
            420                 425                 430         


Phe Leu Val Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Glu Phe Ala 
    450                 455                 460                 


Trp Leu Asn Arg Lys Ser Asp Glu Lys Ile Arg Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Glu Met Val Asp Lys Glu Thr Ser Ala Glu Asn Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Asn Tyr Asp Gln Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Val Thr Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Arg Thr Phe Lys Val Tyr Arg Lys Val Thr 
545                 550                 555                 560 


Lys Glu Lys Leu Met Asp Phe Leu Ser Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Leu Leu Gly Leu Asp Lys Asp Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Val Ser Glu Asp Phe 
        595                 600                 605             


Leu Asp Asn Ser Glu Asn Glu Asp Ile Leu Glu Asn Val Val Leu Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys 
625                 630                 635                 640 


Tyr Lys Asp Val Leu Thr Glu Glu Gln Arg Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Val Thr Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Thr Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu 
    690                 695                 700                 


Ser Phe Val Asp Glu Ile Arg Leu Ala Gln Gly Ser Gly Lys Ala Glu 
705                 710                 715                 720 


Asp Tyr Arg Ala Glu Val Gln Asn Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Ile Glu Val 
            740                 745                 750         


Met Gly Tyr Asp Pro Glu His Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Lys 
    770                 775                 780                 


Ile Glu Asn Ala Ile Lys Asn Leu Asp Ser Asn Leu Asn Ser Lys Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asn Asn Gln Ala Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Glu Glu 
            820                 825                 830         


Leu Asp Ile Asp Gln Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Thr Lys 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val Tyr Lys Lys Lys Asn Phe Trp Gln Gln Leu Leu Asp Ser Gln Leu 
                885                 890                 895     


Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Asn Glu Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Thr Arg Phe 
    930                 935                 940                 


Asn Thr Lys Leu Asp Glu Ala Gly Asn Arg Ile Arg Asp Pro Lys Val 
945                 950                 955                 960 


Asn Ile Ile Ile Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Tyr Gln Leu Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Thr Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Gln  Leu Ala Pro Glu Phe  Val Tyr Gly Glu Tyr  Pro Thr Tyr 
    1010                 1015                 1020             


Asn Ser  Tyr Asn Ser Arg Lys  Ser Ala Thr Glu Lys  Val Leu Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Arg Ile Val  Val His Ser 
    1040                 1045                 1050             


Lys Thr  Gly Glu Val Arg Ile  Arg Pro Val Ile Glu  Val Asn Lys 
    1055                 1060                 1065             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Lys Ser Asn  Phe Arg Thr 
    1070                 1075                 1080             


Val Arg  Lys Val Leu Ser Tyr  Pro Gln Val Asn Val  Val Lys Lys 
    1085                 1090                 1095             


Thr Glu  Val Gln Asn His Gly  Leu Asp Arg Gly Lys  Pro Lys Gly 
    1100                 1105                 1110             


Phe Tyr  Asn Ala Asn Leu Ser  Pro Arg Pro Lys Glu  Gly Ser Val 
    1115                 1120                 1125             


Glu Asn  Leu Val Pro Ala Lys  Gln Ser Phe Asp Thr  Lys Arg Tyr 
    1130                 1135                 1140             


Gly Gly  Tyr Ala Gly Ile Ser  Asn Ser Tyr Ala Val  Leu Val Asn 
    1145                 1150                 1155             


Gly Ile  Ile Glu Lys Gly Lys  Lys Lys Ser Lys Ala  Glu Val Thr 
    1160                 1165                 1170             


Glu Phe  Gln Gly Ile Ser Ile  Leu Ala Arg Lys Asp  Phe Asp Lys 
    1175                 1180                 1185             


Asn Pro  Lys Gln Tyr Leu Leu  Asn Leu Gly Tyr Lys  Asp Ile Lys 
    1190                 1195                 1200             


Ser Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn 
    1205                 1210                 1215             


Gly Ser  Arg Arg Met Leu Ala  Ser Ile Leu Ser Thr  Asn Asn Lys 
    1220                 1225                 1230             


Arg Gly  Glu Ile His Lys Gly  Asn Glu Met Tyr Leu  Pro Asp Lys 
    1235                 1240                 1245             


Phe Val  Thr Leu Leu Tyr His  Ala Met Arg Val Asn  Arg Thr Leu 
    1250                 1255                 1260             


Glu Pro  Gly His Lys Lys Tyr  Val Glu Ala His Arg  His Val Phe 
    1265                 1270                 1275             


Asp Glu  Leu Leu Thr Tyr Ile  Leu Glu Tyr Asn Glu  Lys Ile Val 
    1280                 1285                 1290             


Gly Ala  Lys Ala Asn Gly Lys  Arg Ile Val Glu Ala  Tyr Ser Ala 
    1295                 1300                 1305             


Arg Lys  Asp Thr Asp Asn Leu  Glu Glu Leu Cys Asn  Ser Phe Ile 
    1310                 1315                 1320             


Asn Leu  Leu Asn Leu Thr Ala  Leu Gly Ser Ala Val  Asp Phe Glu 
    1325                 1330                 1335             


Phe Leu  Gly Thr Lys Ile Pro  Arg Tyr Arg Asp Tyr  Thr Pro Ser 
    1340                 1345                 1350             


Ser Leu  Leu Lys Ala Thr Leu  Ile His Gln Ser Val  Thr Gly Leu 
    1355                 1360                 1365             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu Gly Gly  Asp 
    1370                 1375                 1380     


<210>  1104
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1104

Met Val Lys Lys Lys Tyr Ala Ile Gly Ile Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Val Val Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asn Thr Glu Lys Arg Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Thr Leu Leu Phe Asp Glu Gly Asn Thr Ala Glu Asn Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile 
65                  70                  75                  80  


Leu Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Lys Ile Asp Asp 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Ile Val Glu Asp Lys 
            100                 105                 110         


Gln Gly Ser Lys His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Ser Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Lys 
                165                 170                 175     


Ser Asp Asn Lys Asp Val Gln His Leu Phe Asn Glu Phe Val Glu Met 
            180                 185                 190         


Phe Asp Lys Thr Val Glu Gly Ser Tyr Leu Ser Glu Asn Ile Pro Asp 
        195                 200                 205             


Val Ala Asp Ile Leu Val Glu Lys Val Ser Lys Ser Arg Arg Leu Glu 
    210                 215                 220                 


Asn Val Leu Gln Tyr Phe Pro Asn Glu Lys Lys Asn Gly Leu Leu Gly 
225                 230                 235                 240 


Asn Phe Leu Ser Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Glu Leu Ala Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Ile Ala Thr Lys Ser Leu Tyr Asp Gly Ile Leu Leu Ala 
    290                 295                 300                 


Gly Ile Leu Ser Thr Thr Asp Ser Thr Thr Lys Ala Pro Leu Ser Ser 
305                 310                 315                 320 


Ser Met Val Asn Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu 
                325                 330                 335     


Lys Asp Phe Ile Arg Gln Asn Leu Ser Asp Ser Tyr Lys Glu Val Phe 
            340                 345                 350         


Asn Asp Ala Leu Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Thr Ile Glu Lys Ile 
    370                 375                 380                 


Glu Gly Ser Asp Tyr Phe Ile Asp Lys Ile Asp Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Lys Glu Met Gln Ala Ile Ile Ile Arg Gln Ala Lys Phe Tyr Pro 
            420                 425                 430         


Phe Leu Glu Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Glu Phe Ala 
    450                 455                 460                 


Trp Leu Asn Arg Lys Ser Asp Glu Lys Ile Arg Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Glu Ile Val Asp Lys Glu Thr Ser Ala Glu Asn Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Asn Tyr Asp Gln Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Val Thr Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr 
545                 550                 555                 560 


Lys Glu Lys Leu Met Asp Phe Leu Gly Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Leu Leu Gly Leu Asp Lys Asp Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Val Ser Lys Asp Leu 
        595                 600                 605             


Leu Asp Asn Pro Glu Asn Glu Asp Ile Leu Glu Asn Val Val Leu Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys 
625                 630                 635                 640 


Tyr Lys Glu Val Leu Thr Glu Glu Gln Arg Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Val Thr Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Thr Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu 
    690                 695                 700                 


Ser Phe Val Asp Glu Ile Arg Leu Ala Gln Gly Ser Gly Glu Ala Glu 
705                 710                 715                 720 


Asp Tyr Arg Ala Glu Val Gln Asn Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Ile Glu Val 
            740                 745                 750         


Met Gly Tyr Glu Pro Glu His Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Lys 
    770                 775                 780                 


Ile Glu Asn Ala Ile Lys Asn Leu Asp Ser Asn Leu Asn Ser Lys Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asn Asn Gln Ala Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Glu Glu 
            820                 825                 830         


Leu Asp Ile Asp Gln Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Thr Lys 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val Tyr Lys Lys Lys Ser Phe Trp Lys Gln Leu Leu Asp Ser Gln Leu 
                885                 890                 895     


Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Asn Glu Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Met Leu Asp Thr Arg Phe 
    930                 935                 940                 


Asn Thr Lys Leu Asp Glu Ala Gly Asn Arg Ile Arg Asp Pro Lys Val 
945                 950                 955                 960 


Asn Ile Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Tyr Gln Leu Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Thr Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Gln  Leu Ala Pro Glu Phe  Val Tyr Gly Glu Tyr  Pro Thr Tyr 
    1010                 1015                 1020             


Asn Ser  Tyr Asn Ser Arg Lys  Ser Ala Thr Glu Lys  Val Leu Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Arg Ile Val  Val His Ser 
    1040                 1045                 1050             


Lys Thr  Gly Glu Val Arg Ile  Arg Pro Val Ile Glu  Val Asn Lys 
    1055                 1060                 1065             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Lys Ser Asp  Phe Arg Thr 
    1070                 1075                 1080             


Val Arg  Lys Val Leu Ser Tyr  Pro Gln Val Asn Val  Val Lys Lys 
    1085                 1090                 1095             


Thr Glu  Val Gln Asn His Gly  Leu Asp Arg Gly Lys  His Lys Gly 
    1100                 1105                 1110             


Phe Tyr  Asn Ala Asn Leu Ser  Pro Arg Pro Lys Glu  Gly Ser Val 
    1115                 1120                 1125             


Glu Asn  Leu Val Pro Ala Lys  Gln Ser Phe Asp Thr  Lys Arg Tyr 
    1130                 1135                 1140             


Gly Gly  Tyr Ala Gly Ile Ser  Asn Ser Tyr Ala Val  Leu Val Asn 
    1145                 1150                 1155             


Gly Ile  Ile Glu Lys Gly Lys  Lys Lys Ser Lys Ala  Glu Val Thr 
    1160                 1165                 1170             


Glu Phe  Gln Gly Ile Ser Ile  Leu Ala Arg Lys Asp  Phe Asp Lys 
    1175                 1180                 1185             


Asn Pro  Lys Gln Tyr Leu Leu  Asn Leu Gly Tyr Lys  Asp Ile Lys 
    1190                 1195                 1200             


Ser Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn 
    1205                 1210                 1215             


Gly Ser  Arg Arg Met Leu Ala  Ser Ile Leu Ser Thr  Asn Asn Lys 
    1220                 1225                 1230             


Arg Gly  Glu Ile His Lys Gly  Asn Glu Met Tyr Leu  Pro Asp Lys 
    1235                 1240                 1245             


Phe Val  Thr Leu Leu Tyr His  Ala Met Arg Val Asn  Arg Thr Leu 
    1250                 1255                 1260             


Glu Pro  Gly His Lys Lys Tyr  Val Glu Thr His Arg  His Val Phe 
    1265                 1270                 1275             


Asp Glu  Leu Leu Ile Tyr Ile  Leu Glu Tyr Asn Glu  Lys Ile Val 
    1280                 1285                 1290             


Gly Ala  Lys Ala Asn Gly Lys  Arg Ile Val Glu Ala  Tyr Ser Ala 
    1295                 1300                 1305             


Arg Lys  Asp Thr Asp Asn Leu  Glu Glu Leu Cys Asn  Ser Phe Ile 
    1310                 1315                 1320             


Asn Leu  Leu Asn Leu Thr Ala  Leu Gly Ser Ala Val  Asp Phe Glu 
    1325                 1330                 1335             


Phe Leu  Gly Thr Lys Ile Pro  Arg Tyr Arg Asp Tyr  Thr Pro Ser 
    1340                 1345                 1350             


Ser Leu  Leu Lys Ala Thr Leu  Ile His Gln Ser Val  Thr Gly Leu 
    1355                 1360                 1365             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu Gly Gly  Asp 
    1370                 1375                 1380     


<210>  1105
<211>  1381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1105

Met Val Lys Lys Lys Tyr Ala Ile Gly Ile Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ser Val Val Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asn Thr Glu Lys Arg Tyr Ile Lys Lys Asn Leu 
        35                  40                  45              


Leu Gly Thr Leu Leu Phe Asp Glu Gly Asn Thr Ala Glu Asn Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile 
65                  70                  75                  80  


Leu Tyr Leu Gln Glu Ile Phe Ala Glu Glu Ile Asn Lys Ile Asp Asp 
                85                  90                  95      


Ser Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Ile Val Glu Asp Lys 
            100                 105                 110         


Gln Gly Ser Lys His Pro Ile Phe Gly Thr Leu Gln Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Ser Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Ile Ile Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Asp Leu Lys 
                165                 170                 175     


Ser Glu Asn Lys Asp Val Gln His Leu Phe Asn Asp Phe Val Glu Met 
            180                 185                 190         


Phe Asp Lys Thr Val Glu Gly Ser Tyr Leu Ser Glu Asn Leu Pro Asn 
        195                 200                 205             


Val Ala Asp Val Leu Val Glu Lys Val Ser Lys Ser Arg His Leu Glu 
    210                 215                 220                 


Asn Ile Leu His Tyr Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly 
225                 230                 235                 240 


Asn Phe Leu Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn 
                245                 250                 255     


Phe Glu Leu Ala Glu Asp Ala Lys Ile Gln Phe Ser Lys Glu Thr Tyr 
            260                 265                 270         


Glu Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala 
        275                 280                 285             


Asp Leu Phe Ile Ala Thr Lys Ser Leu Tyr Asp Gly Ile Leu Leu Ala 
    290                 295                 300                 


Gly Ile Leu Ser Thr Thr Asn Ser Thr Thr Lys Ala Pro Leu Ser Ser 
305                 310                 315                 320 


Ser Met Val Asn Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu 
                325                 330                 335     


Lys Asn Phe Ile His Gln Asn Leu Ser Asp Ser Tyr Lys Glu Val Phe 
            340                 345                 350         


Asn Asp Lys Leu Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Asn Phe Tyr Arg Phe Ile Lys Lys Ala Ile Glu Lys Ile 
    370                 375                 380                 


Asp Gly Ser Asn Tyr Phe Ile Glu Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Gln Ala Ile Ile Arg Arg Gln Ala Lys Phe Tyr Pro 
            420                 425                 430         


Phe Leu Glu Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Glu Phe Ala 
    450                 455                 460                 


Trp Leu Asn Arg Lys Ser Asp Glu Lys Ile Arg Pro Trp Asn Phe Asp 
465                 470                 475                 480 


Glu Met Val Asp Lys Glu Thr Ser Ala Lys Asn Phe Ile Thr Arg Met 
                485                 490                 495     


Thr Asn Tyr Asp Gln Tyr Leu Pro Asp Gln Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Lys Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Val Thr Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr 
545                 550                 555                 560 


Lys Glu Lys Leu Met Asp Phe Leu Gly Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Leu Leu Gly Leu Asp Lys Glu Asn Lys Ser Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Lys Lys Ile Val Ser Lys Asp Phe 
        595                 600                 605             


Leu Asp Asn Ser Glu Asn Glu Asp Ile Leu Glu Asn Val Val Leu Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys 
625                 630                 635                 640 


Tyr Lys Asp Ile Leu Thr Glu Glu Gln Arg Lys Lys Leu Glu Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Val Thr Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Thr Ser Asn Cys Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu 
    690                 695                 700                 


Ser Phe Val Asp Glu Ile Arg Leu Ala Gln Gly Ser Gly Glu Ala Glu 
705                 710                 715                 720 


Asp Tyr Arg Ala Glu Val Gln Asn Leu Ala Gly Ser Pro Ala Ile Lys 
                725                 730                 735     


Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Ile Glu Val 
            740                 745                 750         


Met Gly Tyr Asp Pro Glu His Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Lys 
    770                 775                 780                 


Ile Glu Asn Ala Ile Lys Asn Leu Asp Ser Asn Leu Asn Ser Lys Ile 
785                 790                 795                 800 


Leu Lys Glu Tyr Pro Thr Asn Asn Gln Ala Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Asp Glu Glu 
            820                 825                 830         


Leu Asp Ile Asp Gln Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ala Phe Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Thr Lys 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile 
865                 870                 875                 880 


Val Tyr Lys Lys Lys Ser Phe Trp Lys Gln Leu Leu Asp Ser Gln Leu 
                885                 890                 895     


Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
            900                 905                 910         


Leu Thr Asn Glu Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys His Val Ala Arg Met Leu Asp Thr Arg Phe 
    930                 935                 940                 


Asn Thr Lys Leu Asp Glu Ala Gly Asn Arg Ile Arg Asp Pro Lys Val 
945                 950                 955                 960 


Asn Ile Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Tyr Gln Leu Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Thr Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Gln  Leu Ala Pro Glu Phe  Val Tyr Gly Glu Tyr  Pro Thr Tyr 
    1010                 1015                 1020             


Asn Ser  Tyr Asn Ser Arg Lys  Ser Ala Thr Glu Lys  Val Leu Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Arg Ile Val  Val His Ser 
    1040                 1045                 1050             


Lys Thr  Gly Glu Val Arg Ile  Arg Pro Val Ile Glu  Val Asn Lys 
    1055                 1060                 1065             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Lys Ser Asp  Phe Arg Thr 
    1070                 1075                 1080             


Val Arg  Lys Val Leu Ser Tyr  Pro Gln Val Asn Val  Val Lys Lys 
    1085                 1090                 1095             


Thr Glu  Val Gln Asn His Gly  Leu Asp Arg Gly Lys  Pro Lys Gly 
    1100                 1105                 1110             


Phe Tyr  Asn Ala Asn Leu Ser  Pro Arg Pro Lys Glu  Gly Ser Val 
    1115                 1120                 1125             


Glu Asn  Leu Val Pro Ala Lys  Gln Ser Phe Asp Thr  Lys Arg Tyr 
    1130                 1135                 1140             


Gly Gly  Tyr Ala Gly Ile Ser  Asn Ser Tyr Ala Val  Leu Val Asn 
    1145                 1150                 1155             


Gly Ile  Ile Glu Lys Gly Lys  Lys Lys Ser Lys Ala  Glu Val Thr 
    1160                 1165                 1170             


Glu Phe  Gln Gly Ile Ser Ile  Leu Ala Arg Lys Asp  Phe Asp Lys 
    1175                 1180                 1185             


Asn Pro  Lys Gln Tyr Leu Leu  Asn Leu Gly Tyr Lys  Asp Ile Lys 
    1190                 1195                 1200             


Ser Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn 
    1205                 1210                 1215             


Gly Ser  Arg Arg Met Leu Ala  Ser Ile Leu Ser Thr  Asn Asn Lys 
    1220                 1225                 1230             


Arg Gly  Glu Ile His Lys Gly  Asn Glu Met Tyr Leu  Pro Asp Lys 
    1235                 1240                 1245             


Phe Val  Thr Leu Leu Tyr His  Ala Met Arg Val Asn  Arg Thr Leu 
    1250                 1255                 1260             


Glu Pro  Gly His Lys Lys Tyr  Val Glu Thr His Arg  His Val Phe 
    1265                 1270                 1275             


Asp Glu  Leu Leu Ile Tyr Ile  Leu Glu Tyr Asn Glu  Lys Ile Val 
    1280                 1285                 1290             


Gly Ala  Lys Ala Asn Gly Lys  Arg Ile Val Glu Ala  Tyr Ser Ala 
    1295                 1300                 1305             


Arg Lys  Asp Thr Asp Asn Leu  Glu Glu Leu Cys Asn  Ser Phe Ile 
    1310                 1315                 1320             


Asn Leu  Leu Asn Leu Thr Ala  Leu Gly Ser Ala Val  Asp Phe Glu 
    1325                 1330                 1335             


Phe Leu  Gly Thr Lys Ile Pro  Arg Tyr Arg Asp Tyr  Thr Pro Ser 
    1340                 1345                 1350             


Ser Leu  Leu Lys Ala Thr Leu  Ile His Gln Ser Val  Thr Gly Leu 
    1355                 1360                 1365             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu Gly Gly  Asp 
    1370                 1375                 1380     


<210>  1106
<211>  1382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1106

Met Lys Lys Ile Val Asn Tyr Asn Leu Gly Leu Asp Ile Gly Thr Ser 
1               5                   10                  15      


Ser Ile Gly Tyr Ala Ala Ile Asp Asp Gln Gly Arg Pro Val Arg His 
            20                  25                  30          


Gln Asn Lys Thr Val Met Gly Val Arg Leu Phe Lys Glu Gly Lys Thr 
        35                  40                  45              


Ala Glu Ser Thr Arg Ile Phe Arg Gly Thr Arg Arg Arg Leu Ser His 
    50                  55                  60                  


Arg His Trp Arg Leu Gly Tyr Leu Asp Arg Ile Phe Asp Asn Glu Ile 
65                  70                  75                  80  


Ala Lys Val Asp Glu Asn Phe Leu Pro Arg Leu Lys Ala Ser Lys Arg 
                85                  90                  95      


Ala Arg Ser Asp Glu Lys Arg Ser Phe Arg Gly Ser Leu Leu Phe Pro 
            100                 105                 110         


Glu Thr Gly Asp Asp Ala Tyr Tyr Gln Lys Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Tyr Ala Leu Ile Thr Gln Lys Lys Gln Phe Asp Ile Arg Leu 
    130                 135                 140                 


Ile Tyr Leu Ala Leu His His Met Ile Lys Tyr Arg Gly Asn Phe Leu 
145                 150                 155                 160 


Asp Thr Thr Pro Val Ser Ala Phe Asn Ile His Asp Ile Asp Leu Thr 
                165                 170                 175     


Asp Gln Phe Ala Ser Leu Asn Asp Cys Tyr Gln Leu Leu Asp Met Asp 
            180                 185                 190         


Phe Glu Phe Gln Phe Asp Leu Asn Asn Ile Glu Gln Val Gln Lys Leu 
        195                 200                 205             


Leu Leu Asp Glu Thr Leu Ser Lys Thr Lys Arg Gln Ala Ala Val Ser 
    210                 215                 220                 


Lys Val Leu Val Met Ser Thr Gly Asn Lys Ala Phe Asp Asp Gln Gln 
225                 230                 235                 240 


Asn Gly Ile Ala Ser Glu Phe Ile Lys Ala Leu Leu Gly Gly Pro Phe 
                245                 250                 255     


Asp Leu Ser Lys Ile Leu Asn Leu Ser Val Ala Asp Val Lys Ser Trp 
            260                 265                 270         


Lys Leu His Met Thr Asp Lys Asp Val Asp Asp Thr Leu Asn Ser Leu 
        275                 280                 285             


Val Glu Glu Leu Asp Glu Asn His Lys Gln Ile Val Asn Leu Leu Gln 
    290                 295                 300                 


Met Ile Tyr Ser Gln Val Met Met Leu Gly Leu Leu Asn Gly Gln Gln 
305                 310                 315                 320 


Ser Ile Ser Ala Ser Met Ile Glu Arg Tyr Asp Asp His Ala Lys His 
                325                 330                 335     


Trp Ala Ala Leu Lys Lys Leu Leu Pro Ala Leu Gly Glu Gln Glu Gln 
            340                 345                 350         


Ser Thr Ile Ile His Ala Tyr Thr Ala Tyr Thr Gly His Ser Arg Thr 
        355                 360                 365             


Lys Gln Leu Ser Arg Ala Glu Phe Tyr Thr Lys Val Asn Ser Ile Leu 
    370                 375                 380                 


Lys Lys His Glu Asn Ile Ala Glu Ala Thr Gly Leu Ile Glu Leu Ile 
385                 390                 395                 400 


Asp Arg Asn Glu Phe Met Pro Lys Gln Arg Asp Ala Val Asn Gly Val 
                405                 410                 415     


Ile Pro His Gln Leu His Gln Arg Glu Leu Gln Leu Ile Ile Gln Asn 
            420                 425                 430         


Gln Gln Lys Tyr Tyr Pro Phe Leu Ala Ala Lys Ala Tyr Pro Asp Ala 
        435                 440                 445             


Asp Glu Thr Met Ile Glu Gln Leu Leu Ser Phe Arg Val Pro Tyr Tyr 
    450                 455                 460                 


Ala Gly Pro Leu Val Asp Arg Asp Phe Ile Asn Glu Ser Ser Asp Gln 
465                 470                 475                 480 


Glu Gln Ala Met Gln Asn Thr Lys Phe Ser Trp Leu Lys Arg Arg Glu 
                485                 490                 495     


Ala Gly Glu Ile Thr Pro Trp Asn Phe Glu Gln Lys Val Asp Val Gln 
            500                 505                 510         


Glu Thr Ala Asn Arg Phe Ile Lys Arg Leu Thr Thr Lys Asp Thr Tyr 
        515                 520                 525             


Leu Met Thr Glu Asp Val Leu Pro Lys Glu Ser Leu Ile Tyr Gln Arg 
    530                 535                 540                 


Phe Glu Val Leu Asn Glu Leu Asn Ala Ile Arg Ile Asn Asp Arg Arg 
545                 550                 555                 560 


Leu Thr Ala Glu Asn Lys Gln Leu Ala Phe Gln Gln Leu Phe Met Ala 
                565                 570                 575     


Gly Asn Thr Lys Asn Val Ser Ile Lys Lys Phe Arg Asn Phe Val Phe 
            580                 585                 590         


Asp Ser Leu Lys Phe Pro Glu Lys Val Lys Ile Thr Gly Phe Ala Gly 
        595                 600                 605             


Gln His Asn Phe Leu Ala Lys Leu Ser Thr Tyr His Asp Phe Lys Lys 
    610                 615                 620                 


Ile Phe Gly Glu Arg Val Asp His Thr Asp Leu Gln Pro Asp Phe Glu 
625                 630                 635                 640 


Arg Ile Val Glu Trp Cys Thr Ile Phe Glu Asp Arg His Ile Tyr Arg 
                645                 650                 655     


Glu Lys Leu Lys Glu Ile Gln Trp Leu Ser Gln Ser Glu Ile Asp Gln 
            660                 665                 670         


Leu Val Leu Ile Arg Asn His Gly Trp Gly Arg Leu Ser Lys Arg Leu 
        675                 680                 685             


Leu Thr Gly Ile Arg Asp Ser Asp Gly Arg Thr Val Met Asp Gln Leu 
    690                 695                 700                 


Trp Phe Gly Thr Asp Asn Phe Met Arg Ile Ile Ser Gln Ser Asp Phe 
705                 710                 715                 720 


Ala Ala Ala Ile Ala Asn Glu Asn Arg Thr Gln Leu Asn Gln His Gly 
                725                 730                 735     


Leu Glu Asp Val Leu Asp Ser Ala Phe Met Ser Pro Gln Asn Lys Lys 
            740                 745                 750         


Ala Ile Arg Gln Val Leu Lys Val Val Asp Asp Ile Glu Gln Thr Leu 
        755                 760                 765             


Gly Cys Pro Pro Lys Lys Val Met Ile Glu Phe Thr Arg Ser Pro Asp 
    770                 775                 780                 


Lys Lys Lys Gln Arg Thr Val Thr Arg Leu Glu Lys Val Gln Gln Ala 
785                 790                 795                 800 


Tyr Lys Ala Val Ala Asp Gln Leu Ala Gly Gln Ser Ile Val Ser Glu 
                805                 810                 815     


Leu Asp Gln Tyr Ala Asn Thr His Ser Lys Leu Ser Asp Thr Tyr Tyr 
            820                 825                 830         


Leu Tyr Phe Met Gln Leu Gly Arg Asp Leu Tyr Ser Gly Lys Pro Ile 
        835                 840                 845             


Asp Ile Asp Ser Pro Met Leu Asp Asp Asp His Ile Leu Pro Arg Ala 
    850                 855                 860                 


Phe Met Lys Asp Asn Ser Leu Asp Asn Arg Ala Leu Thr Glu Lys Pro 
865                 870                 875                 880 


Ile Asn Asn Thr Lys Ala Ala Lys Phe Pro Ser Lys Val Phe Gly Gln 
                885                 890                 895     


Gln Lys Glu Val Thr Asp Phe Trp Lys Leu Leu Arg Arg Cys Gly Leu 
            900                 905                 910         


Met Ser Asn Ile Lys Glu Lys His Leu Tyr Leu Asp Pro Asp Asn Val 
        915                 920                 925             


Asp Lys Trp Thr Arg Arg Gly Phe Ile His Arg Gln Leu Val Glu Thr 
    930                 935                 940                 


Ser Gln Val Ile Lys Ile Val Ala Asn Ile Leu Asn Asp Arg Tyr Gln 
945                 950                 955                 960 


Gln Ser Gln Thr Glu Val Leu Glu Val Pro Ala Arg Leu Gly Thr Glu 
                965                 970                 975     


Leu Arg Glu Cys Phe Gly Trp Tyr Lys Val Arg Glu Val Asn Asp Tyr 
            980                 985                 990         


His His Val Phe Asp Ala Tyr Leu  Ala Ala Phe Ile Gly  Asn Tyr Leu 
        995                 1000                 1005             


Tyr Arg  Arg Tyr Pro Lys Leu  Gln Pro Tyr Phe Val  Tyr Gly Asn 
    1010                 1015                 1020             


Phe Ala  Glu Leu Asn Lys Ala  Gln Leu Lys Asn Ile  Lys Ser Phe 
    1025                 1030                 1035             


Asn Phe  Leu Tyr Asn Ile Thr  Asp Asn Lys Arg Val  Val Lys Asp 
    1040                 1045                 1050             


Ile Glu  Thr Gly Gln Thr Leu  Trp Asp Asp Gln Ile  Arg Gln Gln 
    1055                 1060                 1065             


Ile Gln  His Ile Tyr Glu Tyr  Lys Tyr Met Leu Thr  Ser His Glu 
    1070                 1075                 1080             


Ala Phe  Thr Gln His Lys Glu  Leu Tyr Gly Gln Thr  Ile Tyr Pro 
    1085                 1090                 1095             


Arg Arg  Glu Phe Lys Pro Gly  Thr Leu Lys Ala Ile  Lys Gln Asp 
    1100                 1105                 1110             


Gln Pro  Gln Glu Leu Tyr Gly  Gly Tyr Lys Asn Leu  Thr Ala Ala 
    1115                 1120                 1125             


Tyr Met  Cys Ile Val Lys Ile  Gln Ala Arg Lys Arg  Arg Ala Ala 
    1130                 1135                 1140             


Glu Tyr  Arg Val Val Arg Val  Ala Thr Glu Ser Leu  Ser Ala Leu 
    1145                 1150                 1155             


Gln Gln  Ala Lys Arg Leu Gly  Val Gln Ala Tyr Glu  Arg Cys Leu 
    1160                 1165                 1170             


Arg Gln  Glu Leu Val Arg Asn  Asn Glu Ile Leu Arg  Thr Asn Ala 
    1175                 1180                 1185             


Asp Pro  Asn Ser Ala Pro Arg  Glu Phe Glu Val Leu  Leu Asp Arg 
    1190                 1195                 1200             


Val Leu  Cys His Gln Met Ile  Ile Ser Asp Gly Gln  Lys Tyr Leu 
    1205                 1210                 1215             


Leu Gly  Ser His Ala Tyr Lys  Ile Asn Cys Met Gln  Leu Val Leu 
    1220                 1225                 1230             


Ser Asn  Asn Ser Met Lys Ile  Leu Ser Thr Ser Ile  Val Lys Leu 
    1235                 1240                 1245             


Lys Gln  Met Thr Asn Glu Pro  Leu Val Gln Val Tyr  Arg Glu Ile 
    1250                 1255                 1260             


Leu Asp  Val Val Asn Lys Tyr  Phe Thr Leu Tyr Asp  Val Asn Ser 
    1265                 1270                 1275             


Phe Arg  Glu Asn Leu Asn Gln  Phe Phe Asp Lys Phe  Arg Thr Leu 
    1280                 1285                 1290             


Pro Ile  Thr Asn Glu Tyr Glu  Ser Ser Lys Ser Gly  Lys Lys Leu 
    1295                 1300                 1305             


Ile His  Arg Gly Gln Leu Glu  Thr Leu Thr Leu Ile  Leu Arg Gly 
    1310                 1315                 1320             


Leu His  Ala Asn Ser Asp Lys  Val Gln Tyr Gly Asp  Gln Ser Thr 
    1325                 1330                 1335             


Leu Leu  Gly Gly Phe Gln Thr  Lys Asp Gly Ile Lys  Leu Ser Lys 
    1340                 1345                 1350             


Asp Ser  Val Ile Gln Tyr Thr  Ser Pro Ala Gly Leu  Asn Val Arg 
    1355                 1360                 1365             


Thr Val  Lys Leu Arg Asp Leu  Glu Gln Lys Glu Ala  Gln Lys 
    1370                 1375                 1380         


<210>  1107
<211>  1382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1107

Met Val Asp Glu Lys Ile Phe Trp Gly Leu Asp Ile Gly Thr Asp Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Thr Asn Ser Glu Tyr Lys Leu Lys Lys Tyr Lys 
            20                  25                  30          


Asn Asn Leu Met Trp Gly Val His Leu Phe Asp Glu Ala Lys Gln Ser 
        35                  40                  45              


Ala Glu Arg Arg Ser Phe Arg Thr Ala Arg Arg Arg Leu Asp Arg Arg 
    50                  55                  60                  


Lys Gln Arg Ile Ile Leu Leu Gln Glu Phe Phe Val Arg Ala Val Cys 
65                  70                  75                  80  


Glu Lys Asp Glu Asn Phe Phe Arg Arg Leu Lys Glu Ser Ala Leu Leu 
                85                  90                  95      


Pro Glu Asp Ala Glu His Arg Thr Asn Asn Ile Phe Phe Asp Asp Pro 
            100                 105                 110         


Asp Tyr Thr Asp Lys Asp Tyr Phe Glu Glu Tyr Pro Thr Ile His His 
        115                 120                 125             


Leu Ile Cys Glu Leu Met Glu Ser Lys Glu Pro His Asp Val Arg Leu 
    130                 135                 140                 


Val Tyr Leu Ala Cys Val Tyr Leu Leu Ala His Arg Gly His Phe Leu 
145                 150                 155                 160 


Leu Pro Val Ser Glu Asp Asp Ile Ser Lys Val Thr Glu Phe Glu Pro 
                165                 170                 175     


Leu Tyr Glu Ser Phe Tyr Lys Ala Leu Glu Glu Lys Leu Asp Asp Glu 
            180                 185                 190         


Pro Pro Phe Asp Arg Ser Ala Asp Asp Phe Ala Glu Ile Leu Lys Ser 
        195                 200                 205             


His Lys Thr Val Ser Ala Lys Asn Lys Asp Phe Asp Lys Leu Leu Phe 
    210                 215                 220                 


Gly Gly Lys Val Lys Thr Tyr Asp Asn Glu Asn Ile Ser Tyr Ser Ala 
225                 230                 235                 240 


Leu Ile Lys Leu Leu Ser Gly Gly Thr Glu Lys Leu Ser Lys Phe Phe 
                245                 250                 255     


Ala Asn Glu Glu Tyr Thr Asp Leu Glu Lys Asp Ser Val Cys Val Arg 
            260                 265                 270         


Asn Ala Asp Phe Gly Asp Thr Leu Glu Met Leu Glu Gly Gln Ile Asp 
        275                 280                 285             


Glu Leu Asp Phe Ala Leu Leu Lys Ser Val Lys Ser Leu Tyr Asp Trp 
    290                 295                 300                 


Ser Leu Leu Val Asp Ile Leu Glu Gly Lys Ser Leu Ile Ser Glu Ala 
305                 310                 315                 320 


Lys Lys Asp Lys Tyr Asp Glu His Gly Tyr Asp Leu Asp Ala Leu Lys 
                325                 330                 335     


Tyr Leu Phe Arg Glu Tyr Leu Thr Lys Asp Asp Tyr Asn Glu Met Phe 
            340                 345                 350         


Lys Glu Val Ser Gly Lys Gln Asn Tyr Ala Ser Tyr Val Tyr Asn Ala 
        355                 360                 365             


Ser Ser Asp Lys Thr Arg Asp Ser Lys Tyr Lys Lys Cys Ser Gln Lys 
    370                 375                 380                 


Asp Phe Cys Lys Phe Thr Lys Lys Phe Leu Ser Lys Ile Lys Pro Asn 
385                 390                 395                 400 


Glu Lys Asp Lys Pro Cys Leu Asp Lys Leu Leu Glu Lys Cys Glu Gln 
                405                 410                 415     


Asn Ser Leu Cys Pro Lys Gln Val Thr Thr Asp Asn Arg Val Ile Pro 
            420                 425                 430         


Tyr Gln Leu Tyr Tyr Val Glu Leu Lys Lys Ile Leu Glu Asn Ala Cys 
        435                 440                 445             


Gly Tyr Leu Pro Phe Leu Asn Glu Arg Asp Glu Tyr Gly Thr Val Ala 
    450                 455                 460                 


Asp Lys Ile Leu Ser Ile Met Lys Phe Arg Val Pro Tyr Tyr Val Gly 
465                 470                 475                 480 


Pro Leu Val Asp Ser Lys Lys Ser Pro Asn Ala Trp Leu Val Arg Lys 
                485                 490                 495     


Leu Asp Gly Lys Ile Thr Pro Trp Asn Phe Thr Asp Met Val Asn Glu 
            500                 505                 510         


Asp Glu Ser Glu Asn Ala Phe Ile Arg Arg Met Thr Cys Lys Cys Thr 
        515                 520                 525             


Tyr Val Ala Gly Gln Asp Val Leu Pro Lys Tyr Ser Leu Leu Tyr Ser 
    530                 535                 540                 


Lys Phe Ser Val Leu Asn Glu Ile Asn Asn Ile Lys Leu Asn Gly Glu 
545                 550                 555                 560 


Pro Ile Ser Val Gln Thr Lys Gln Glu Ile Tyr Thr Glu Leu Phe Glu 
                565                 570                 575     


Arg Asn Lys Ser Arg Val Ser Lys Lys Lys Ile Arg Asp Cys Leu Ile 
            580                 585                 590         


Ser His Gly Tyr Ala Ala Asp Ser Asp Glu Val Thr Gly Ile Asp Asp 
        595                 600                 605             


Ile Ala Lys Ser Ala Leu Arg Ser Tyr His Asp Phe Lys Lys Met Leu 
    610                 615                 620                 


Ser Asn Gly Ile Leu Thr Glu Gln Gln Val Glu Glu Ile Ile Glu His 
625                 630                 635                 640 


Ile Thr Val Thr Thr Asp Asn Ile Arg Leu Lys Lys Trp Leu Lys Thr 
                645                 650                 655     


Gln Phe Pro Met Leu Ala Asp Glu Asp Val Lys Tyr Ile Thr Lys Leu 
            660                 665                 670         


Lys Tyr Lys Asp Tyr Gly Arg Leu Ser Arg Cys Phe Leu Glu Asp Val 
        675                 680                 685             


Leu Pro Val Asp Thr Lys Thr Gly Glu Ala Glu Ser Asp Lys Asn Ile 
    690                 695                 700                 


Ile Thr Met Leu Trp Glu Thr Asn Glu Asn Ile Met Gln Leu Leu Ser 
705                 710                 715                 720 


Ser Lys Tyr Arg Tyr Ser Glu Asn Ile Glu His Met Asn Arg Gln Tyr 
                725                 730                 735     


Tyr Ala Leu Pro Glu Asn His Lys Ser Met Ser Glu Arg Leu Lys Asp 
            740                 745                 750         


Met Tyr Val Pro Thr Ala Val Arg Arg Ala Val Thr Arg Thr Phe Asp 
        755                 760                 765             


Ile Val Lys Glu Leu Lys Lys Ile Gln Gly Arg Asp Pro Asp Lys Ile 
    770                 775                 780                 


Phe Ile Glu Met Ala Arg Gly Thr Gly Glu Thr Pro Lys Gly Lys Arg 
785                 790                 795                 800 


Thr Asn Ser Arg Lys Asp Gln Ile Leu Glu His Trp His Gly Leu Asp 
                805                 810                 815     


Asn Lys Asp Ile Asn Asp Leu Lys Lys Ser Gly Ile Trp Glu His Leu 
            820                 825                 830         


Asp Thr Ile Asp Asp Ala Lys Leu Arg Ser Asp Lys Tyr Phe Leu Tyr 
        835                 840                 845             


Phe Met Gln Leu Gly Arg Cys Met Tyr Thr Glu Lys Pro Ile Pro Phe 
    850                 855                 860                 


Glu Glu Val Glu Asn Glu His Lys Trp Asn Ile Asp His Ile Trp Pro 
865                 870                 875                 880 


Gln Ala Lys Ile Lys Asp Asp Ser Leu Asp Asn Lys Val Leu Val Ser 
                885                 890                 895     


Ser Asn Glu Asn Gly Lys Lys Ser Asp Ser Tyr Pro Ile Ser Asp Asp 
            900                 905                 910         


Ile Arg His Ser Met Ala Gly Leu Trp His Ser Leu Tyr Lys Lys Gly 
        915                 920                 925             


Leu Ile Ser Glu Lys Lys Tyr Gln Arg Leu Thr Arg Ser Thr Pro Phe 
    930                 935                 940                 


Thr Asp Asp Glu Leu Ser Gly Phe Ile Ala Arg Gln Leu Val Glu Thr 
945                 950                 955                 960 


Arg Gln Ser Thr Lys Ala Val Ala Thr Leu Leu Lys Glu Gln Phe Pro 
                965                 970                 975     


Asn Thr Glu Ile Val Tyr Val Lys Ala Gly Leu Val Ser Asp Phe Arg 
            980                 985                 990         


Gln Glu Met Gly Met Leu Lys Cys  Arg Glu Val Asn Asp  Leu His His 
        995                 1000                 1005             


Ala Lys  Asp Ala Tyr Leu Asn  Ile Val Leu Gly Asn  Val Tyr Asn 
    1010                 1015                 1020             


Thr Arg  Phe Thr Lys Asp Pro  Leu Asn Phe Val Lys  Asn Asn Glu 
    1025                 1030                 1035             


Lys Tyr  Ser Ile Lys Ile Phe  Gln Lys Asn Ser Asp  Gly Lys Lys 
    1040                 1045                 1050             


Thr Gly  Val Ile Thr Arg Lys  Val Glu Arg Gly Gly  Glu Val Ala 
    1055                 1060                 1065             


Trp Asp  Pro Glu Thr Ser Phe  Ala Ile Val Arg Lys  Met Met Ser 
    1070                 1075                 1080             


Lys Asn  Ser Ile Arg Tyr Val  Arg Tyr Ala Tyr Met  Arg Lys Ser 
    1085                 1090                 1095             


Gly Gln  Asn Gly Gly Phe Phe  Asp Gln Asn Pro Leu  Arg Lys Gly 
    1100                 1105                 1110             


Lys Gly  Met Val Ser Ile Lys  His Asn Leu Ser Ile  Glu Lys Tyr 
    1115                 1120                 1125             


Gly Gly  Tyr Asp Asn Ala Thr  Ile Ser Phe Phe Ser  Leu Val Ser 
    1130                 1135                 1140             


Ile Lys  Asp Phe Gly Val Ile  Leu Leu Ala Val Glu  Leu Leu Ser 
    1145                 1150                 1155             


Ala Asn  Lys Phe Leu Lys Asn  Ile Ser Glu Ala Lys  Glu Tyr Ala 
    1160                 1165                 1170             


Ala Asp  Lys Leu Ser Lys Glu  Leu Asn Val Lys Ile  Val Pro Asn 
    1175                 1180                 1185             


Asp Val  Ala Phe Pro Leu Asn  Asn Arg Tyr Asn Val  Val Lys Ile 
    1190                 1195                 1200             


Asn Ala  Val Leu Glu Leu Asp  Gly Tyr Arg Val Met  Ile Ala Ala 
    1205                 1210                 1215             


Lys Gln  Asn Ser Ser Ile Lys  Phe Val Ser Asn Glu  Ser Leu Ile 
    1220                 1225                 1230             


Leu Ser  Ser Gln His Met Ala  Tyr Val Lys Lys Ile  Thr Lys Phe 
    1235                 1240                 1245             


Ile Glu  Lys Arg Lys Gln Asp  Lys Val Cys Met Ile  Asn Ala Phe 
    1250                 1255                 1260             


Ser Gly  Ile Ser Ile Glu Asp  Asn Ile Glu Leu Tyr  Asp Val Leu 
    1265                 1270                 1275             


Val Ser  Lys Cys Asn Ile Lys  Pro Phe Asn Ile Met  Tyr Glu Asn 
    1280                 1285                 1290             


Leu Gly  Ile Lys Leu Ser Asp  Asn Arg Asp Asn Phe  Ile Lys Lys 
    1295                 1300                 1305             


Ser Val  Glu Asn Gln Val Leu  Ile Leu Ser Glu Met  Val Arg Leu 
    1310                 1315                 1320             


Phe Lys  Thr Gly Arg Lys Ala  Gly Val Asn Leu Glu  Glu Val Gly 
    1325                 1330                 1335             


Gly Arg  Lys Asn Ser Gly Val  Lys Thr Leu Asn Thr  Cys Leu Tyr 
    1340                 1345                 1350             


Lys Val  Lys Asp Tyr Ser Asn  Ile Phe Leu Val Asp  Gln Ser Pro 
    1355                 1360                 1365             


Thr Gly  Leu Ile Glu His Lys  Ser Pro Asn Leu Leu  Asp Leu 
    1370                 1375                 1380         


<210>  1108
<211>  1384
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1108

Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Ile Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Asn Thr Ala Ala Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Glu Glu Asp Lys Arg 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Gln Glu Glu Lys Asp Tyr 
        115                 120                 125             


His Glu Lys Phe Ser Thr Ile Tyr His Leu Arg Lys Glu Leu Ala Asp 
    130                 135                 140                 


Lys Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Ile Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Ser Phe Asp 
                165                 170                 175     


Val Arg Asn Thr Asp Ile Ser Lys Gln Tyr Gln Asp Phe Leu Glu Ile 
            180                 185                 190         


Phe Asn Thr Thr Phe Glu Asn Asn Asp Leu Leu Ser Gln Asn Val Asp 
        195                 200                 205             


Val Glu Ala Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp 
    210                 215                 220                 


Arg Ile Leu Ala Gln Tyr Pro Asn Gln Lys Ser Thr Gly Ile Phe Ala 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr 
                245                 250                 255     


Phe Asn Leu Glu Asp Lys Thr Pro Leu Gln Phe Ala Lys Asp Ser Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Phe Ala 
        275                 280                 285             


Asp Leu Phe Ser Ala Ala Lys Lys Leu Tyr Asp Ser Val Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Ile Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Gln Arg Tyr Asp Glu His Arg Glu Asp Leu Lys Gln Leu 
                325                 330                 335     


Lys Gln Phe Val Lys Ala Ser Leu Pro Glu Lys Tyr Gln Glu Ile Phe 
            340                 345                 350         


Ala Asp Ser Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr 
        355                 360                 365             


Asn Gln Glu Ala Phe Tyr Lys Tyr Leu Ser Lys Leu Leu Thr Lys Gln 
    370                 375                 380                 


Glu Asp Ser Glu Asn Phe Leu Glu Lys Ile Lys Asn Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His 
                405                 410                 415     


Leu Thr Glu Leu Lys Ala Ile Ile Arg Arg Gln Ser Glu Tyr Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Ile Gly Pro Leu Ala Arg Glu Lys Ser Asp Phe Ala 
    450                 455                 460                 


Trp Met Thr Arg Lys Thr Asp Asp Ser Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Asp Leu Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile His Arg Met 
                485                 490                 495     


Thr Asn Asn Asp Phe Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Ile Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Arg Tyr Lys Asn Glu Gln Gly Glu Thr Tyr Phe Phe Asp Ser Asn Ile 
    530                 535                 540                 


Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Glu His Arg Lys Val Ser 
545                 550                 555                 560 


Lys Lys Lys Leu Leu Asp Phe Leu Ala Lys Glu Tyr Glu Glu Phe Arg 
                565                 570                 575     


Ile Val Asp Val Ile Gly Leu Asp Lys Glu Asn Lys Ala Phe Asn Ala 
            580                 585                 590         


Ser Leu Gly Thr Tyr His Asp Leu Glu Lys Ile Leu Asp Lys Asp Phe 
        595                 600                 605             


Leu Asp Asn Pro Asp Asn Glu Ser Ile Leu Glu Asp Ile Val Gln Thr 
    610                 615                 620                 


Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Asn 
625                 630                 635                 640 


Tyr Lys Asp Leu Phe Thr Glu Ser Gln Leu Lys Lys Leu Tyr Arg Arg 
                645                 650                 655     


His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile 
            660                 665                 670         


Arg Asp Lys Glu Ser Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp 
        675                 680                 685             


Gly Arg Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Arg 
    690                 695                 700                 


Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
705                 710                 715                 720 


Lys Ser Ile Ile Ser Lys Ala Gln Ala Gly Ser His Ser Asp Asn Leu 
                725                 730                 735     


Lys Glu Val Val Gly Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
            740                 745                 750         


Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
        755                 760                 765             


Tyr Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr 
    770                 775                 780                 


Thr Asn Gln Gly Arg Arg Asn Ser Arg Gln Arg Tyr Lys Leu Leu Asp 
785                 790                 795                 800 


Asp Gly Val Lys Asn Leu Ala Ser Asp Leu Asn Gly Asn Ile Leu Lys 
                805                 810                 815     


Glu Tyr Pro Thr Asp Asn Gln Ala Leu Gln Asn Glu Arg Leu Phe Leu 
            820                 825                 830         


Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Ala Leu Asp 
        835                 840                 845             


Ile Asp Asn Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala 
    850                 855                 860                 


Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala 
865                 870                 875                 880 


Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Ile Val Lys 
                885                 890                 895     


Asp Cys Lys Val Phe Trp Lys Lys Leu Leu Asp Ala Lys Leu Met Ser 
            900                 905                 910         


Gln Arg Lys Tyr Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
        915                 920                 925             


Ser Asp Asp Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg 
    930                 935                 940                 


Gln Ile Thr Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Asn 
945                 950                 955                 960 


Glu Leu Asp Ser Lys Gly Arg Arg Ile Arg Lys Val Lys Ile Val Thr 
                965                 970                 975     


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Gly Phe Tyr 
            980                 985                 990         


Lys Ile Arg Glu Val Asn Asn Tyr  His His Ala His Asp  Ala Tyr Leu 
        995                 1000                 1005             


Asn Ala  Val Val Ala Lys Ala  Ile Leu Thr Lys Tyr  Pro Gln Leu 
    1010                 1015                 1020             


Glu Pro  Glu Phe Val Tyr Gly  Asp Tyr Pro Lys Tyr  Asn Ser Tyr 
    1025                 1030                 1035             


Lys Thr  Arg Lys Ser Ala Thr  Glu Lys Leu Phe Phe  Tyr Ser Asn 
    1040                 1045                 1050             


Ile Met  Asn Phe Phe Lys Thr  Lys Val Thr Leu Ala  Asp Gly Thr 
    1055                 1060                 1065             


Val Val  Val Lys Asp Asp Ile  Glu Val Asn Asn Asp  Thr Gly Glu 
    1070                 1075                 1080             


Ile Val  Trp Asp Lys Lys Lys  His Phe Ala Thr Val  Arg Lys Val 
    1085                 1090                 1095             


Leu Ser  Tyr Pro Gln Asn Asn  Ile Val Lys Lys Thr  Glu Ile Gln 
    1100                 1105                 1110             


Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu Ala His  Gly Asn Ser 
    1115                 1120                 1125             


Asp Lys  Leu Ile Pro Arg Lys  Thr Lys Asp Ile Tyr  Leu Asp Pro 
    1130                 1135                 1140             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Ile Val Ala  Tyr Ser Val 
    1145                 1150                 1155             


Leu Val  Val Ala Asp Ile Lys  Lys Gly Lys Ala Gln  Lys Leu Lys 
    1160                 1165                 1170             


Thr Val  Thr Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Arg 
    1175                 1180                 1185             


Phe Glu  Lys Asn Pro Ser Ala  Phe Leu Glu Ser Lys  Gly Tyr Leu 
    1190                 1195                 1200             


Asn Ile  Arg Ala Asp Lys Leu  Ile Ile Leu Pro Lys  Tyr Ser Leu 
    1205                 1210                 1215             


Phe Glu  Leu Glu Asn Gly Arg  Arg Arg Leu Leu Ala  Ser Ala Gly 
    1220                 1225                 1230             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Thr  Gln Phe Met 
    1235                 1240                 1245             


Lys Phe  Leu Tyr Leu Ala Ser  Arg Tyr Asn Glu Ser  Lys Gly Lys 
    1250                 1255                 1260             


Pro Glu  Glu Ile Glu Lys Lys  Gln Glu Phe Val Asn  Gln His Val 
    1265                 1270                 1275             


Ser Tyr  Phe Asp Asp Ile Leu  Gln Leu Ile Asn Asp  Phe Ser Lys 
    1280                 1285                 1290             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Glu Lys Ile  Asn Lys Leu 
    1295                 1300                 1305             


Tyr Gln  Asp Asn Lys Glu Asn  Ile Ser Val Asp Glu  Leu Ala Asn 
    1310                 1315                 1320             


Asn Ile  Ile Asn Leu Phe Thr  Phe Thr Ser Leu Gly  Ala Pro Ala 
    1325                 1330                 1335             


Ala Phe  Lys Phe Phe Asp Lys  Ile Val Asp Arg Lys  Arg Tyr Thr 
    1340                 1345                 1350             


Ser Thr  Lys Glu Val Leu Asn  Ser Thr Leu Ile His  Gln Ser Ile 
    1355                 1360                 1365             


Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Gly Lys  Leu Gly Glu 
    1370                 1375                 1380             


Asp 
    


<210>  1109
<211>  1384
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1109

Met Lys Leu Arg Gly Ile Glu Asp Asp Tyr Ser Ile Gly Leu Asp Met 
1               5                   10                  15      


Gly Thr Ser Ser Val Gly Trp Ala Val Thr Asp Glu Arg Gly Thr Leu 
            20                  25                  30          


Ala His Phe Lys Arg Lys Pro Thr Trp Gly Ser Arg Leu Phe Arg Glu 
        35                  40                  45              


Ala Gln Thr Ala Ala Val Ala Arg Met Pro Arg Gly Gln Arg Arg Arg 
    50                  55                  60                  


Tyr Val Arg Arg Arg Trp Arg Leu Asp Leu Leu Gln Lys Leu Phe Glu 
65                  70                  75                  80  


Gln Gln Met Glu Gln Ala Asp Pro Asp Phe Phe Ile Arg Leu Arg Gln 
                85                  90                  95      


Ser Arg Leu Leu Arg Asp Asp Arg Ala Glu Glu His Ala Asp Tyr Arg 
            100                 105                 110         


Trp Pro Leu Phe Asn Asp Cys Lys Phe Thr Glu Arg Asp Tyr Tyr Gln 
        115                 120                 125             


Arg Phe Pro Thr Ile Tyr His Val Arg Ser Trp Leu Met Glu Thr Asp 
    130                 135                 140                 


Glu Gln Ala Asp Ile Arg Leu Ile Tyr Leu Ala Leu His Asn Ile Val 
145                 150                 155                 160 


Lys His Arg Gly Asn Phe Leu Arg Glu Gly Gln Ser Leu Ser Ala Lys 
                165                 170                 175     


Ser Ala Arg Pro Asp Glu Ala Leu Asn His Leu Arg Glu Thr Leu Arg 
            180                 185                 190         


Val Trp Ser Ser Glu Arg Gly Phe Glu Cys Ser Ile Ala Asp Asn Gly 
        195                 200                 205             


Ser Ile Leu Ala Met Leu Thr His Pro Asp Leu Ser Pro Ser Asp Arg 
    210                 215                 220                 


Arg Lys Lys Ile Ala Pro Leu Phe Asp Val Lys Ser Asp Asp Ala Ala 
225                 230                 235                 240 


Ala Asp Lys Lys Leu Gly Ile Ala Leu Ala Gly Ala Val Ile Gly Leu 
                245                 250                 255     


Lys Thr Glu Phe Lys Asn Ile Phe Gly Asp Phe Pro Cys Glu Asp Ser 
            260                 265                 270         


Ser Ile Tyr Leu Ser Asn Asp Glu Ala Val Asp Ala Val Arg Ser Ala 
        275                 280                 285             


Cys Pro Asp Asp Cys Ala Glu Leu Phe Asp Arg Leu Cys Glu Val Tyr 
    290                 295                 300                 


Ser Ala Tyr Val Leu Gln Gly Leu Leu Ser Tyr Ala Pro Gly Gln Thr 
305                 310                 315                 320 


Ile Ser Ala Asn Met Val Glu Lys Tyr Arg Arg Tyr Gly Glu Asp Leu 
                325                 330                 335     


Ala Leu Leu Lys Lys Leu Val Lys Ile Tyr Ala Pro Asp Gln Tyr Arg 
            340                 345                 350         


Met Phe Phe Ser Gly Ala Thr Tyr Pro Gly Thr Gly Ile Tyr Asp Ala 
        355                 360                 365             


Ala Gln Ala Arg Gly Tyr Thr Lys Tyr Asn Leu Gly Pro Lys Lys Ser 
    370                 375                 380                 


Glu Tyr Lys Pro Ser Glu Ser Met Gln Tyr Asp Asp Phe Arg Lys Ala 
385                 390                 395                 400 


Val Glu Lys Leu Phe Ala Lys Thr Asp Ala Arg Ala Asp Glu Arg Tyr 
                405                 410                 415     


Arg Met Met Met Asp Arg Phe Asp Lys Gln Gln Phe Leu Arg Arg Leu 
            420                 425                 430         


Lys Thr Ser Asp Asn Gly Ser Ile Tyr His Gln Leu His Leu Glu Glu 
        435                 440                 445             


Leu Lys Ala Ile Val Glu Asn Gln Gly Arg Phe Tyr Pro Phe Leu Lys 
    450                 455                 460                 


Arg Asp Ala Asp Lys Leu Val Ser Leu Val Ser Phe Arg Ile Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ser Thr Arg Asn Ala Arg Thr Asp Gln His Gly 
                485                 490                 495     


Glu Asn Arg Phe Ala Trp Ser Glu Arg Lys Pro Gly Met Gln Asp Glu 
            500                 505                 510         


Pro Ile Phe Pro Trp Asn Trp Glu Ser Ile Ile Asp Arg Ser Lys Ser 
        515                 520                 525             


Ala Glu Lys Phe Ile Leu Arg Met Thr Gly Met Cys Thr Tyr Leu Gln 
    530                 535                 540                 


Gln Glu Pro Val Leu Pro Lys Ser Ser Leu Leu Tyr Glu Glu Phe Cys 
545                 550                 555                 560 


Val Leu Asn Glu Leu Asn Gly Ala His Trp Ser Ile Asp Gly Asp Asp 
                565                 570                 575     


Glu His Arg Phe Asp Ala Ala Asp Arg Glu Gly Ile Ile Glu Glu Leu 
            580                 585                 590         


Phe Arg Arg Lys Arg Thr Val Ser Tyr Gly Asp Val Ala Gly Trp Met 
        595                 600                 605             


Glu Arg Glu Arg Asn Gln Ile Gly Ala His Val Cys Gly Gly Gln Gly 
    610                 615                 620                 


Glu Lys Gly Phe Glu Ser Lys Leu Gly Ser Tyr Ile Phe Phe Cys Lys 
625                 630                 635                 640 


Asp Val Phe Lys Val Glu Arg Leu Glu Gln Ser Asp Tyr Pro Met Ile 
                645                 650                 655     


Glu Arg Ile Ile Leu Trp Asn Thr Leu Phe Glu Asp Arg Lys Ile Leu 
            660                 665                 670         


Ser Gln Arg Leu Lys Glu Glu Tyr Gly Ser Arg Leu Ser Ala Glu Gln 
        675                 680                 685             


Ile Lys Thr Ile Cys Lys Lys Arg Phe Thr Gly Trp Gly Arg Leu Ser 
    690                 695                 700                 


Glu Lys Phe Leu Thr Gly Ile Thr Val Gln Val Asp Glu Asp Ser Val 
705                 710                 715                 720 


Ser Ile Met Asp Val Leu Arg Glu Gly Cys Pro Val Ser Gly Lys Arg 
                725                 730                 735     


Gly Arg Ala Met Val Met Met Glu Ile Leu Arg Asp Glu Glu Leu Gly 
            740                 745                 750         


Phe Gln Lys Lys Val Asp Asp Phe Asn Arg Ala Phe Phe Ala Glu Asn 
        755                 760                 765             


Ala Gln Ala Leu Gly Val Asn Glu Leu Pro Gly Ser Pro Ala Val Arg 
    770                 775                 780                 


Arg Ser Leu Asn Gln Ser Ile Arg Ile Val Asp Glu Ile Ala Ser Ile 
785                 790                 795                 800 


Ala Gly Lys Ala Pro Ala Asn Ile Phe Ile Glu Val Thr Arg Asp Glu 
                805                 810                 815     


Asp Pro Lys Lys Lys Gly Arg Arg Thr Lys Arg Arg Tyr Asn Asp Leu 
            820                 825                 830         


Lys Asp Ala Leu Glu Ala Phe Lys Lys Glu Asp Pro Glu Leu Trp Arg 
        835                 840                 845             


Glu Leu Cys Glu Thr Ala Pro Asn Asp Met Asp Glu Arg Leu Ser Leu 
    850                 855                 860                 


Tyr Phe Met Gln Arg Gly Lys Cys Leu Tyr Ser Gly Arg Ala Ile Asp 
865                 870                 875                 880 


Ile His Gln Leu Ser Asn Ala Gly Ile Tyr Glu Val Asp His Ile Ile 
                885                 890                 895     


Pro Arg Thr Tyr Val Lys Asp Asp Ser Leu Glu Asn Lys Ala Leu Val 
            900                 905                 910         


Tyr Arg Glu Glu Asn Gln Arg Lys Thr Asp Met Leu Leu Ile Asp Pro 
        915                 920                 925             


Glu Ile Arg Arg Arg Met Ser Gly Tyr Trp Arg Met Leu His Glu Ala 
    930                 935                 940                 


Lys Leu Ile Gly Asp Lys Lys Phe Arg Asn Leu Leu Arg Ser Arg Ile 
945                 950                 955                 960 


Asp Asp Lys Ala Leu Lys Gly Phe Ile Ala Arg Gln Leu Val Glu Thr 
                965                 970                 975     


Gly Gln Met Val Lys Leu Val Arg Ser Leu Leu Glu Ala Arg Tyr Pro 
            980                 985                 990         


Glu Thr Asn Ile Ile Ser Val Lys  Ala Ser Ile Ser His  Asp Leu Arg 
        995                 1000                 1005             


Thr Ala  Ala Glu Leu Val Lys  Cys Arg Glu Ala Asn  Asp Phe His 
    1010                 1015                 1020             


His Ala  His Asp Ala Phe Leu  Ala Cys Arg Val Gly  Leu Phe Ile 
    1025                 1030                 1035             


Gln Lys  Arg His Pro Cys Val  Tyr Glu Asn Pro Ile  Gly Leu Ser 
    1040                 1045                 1050             


Gln Val  Val Arg Asn Tyr Val  Arg Gln Gln Ala Asp  Ile Phe Lys 
    1055                 1060                 1065             


Arg Cys  Arg Thr Ile Pro Gly  Ser Ser Gly Phe Ile  Val Asn Ser 
    1070                 1075                 1080             


Phe Met  Thr Ser Gly Phe Asp  Lys Glu Thr Gly Glu  Ile Phe Lys 
    1085                 1090                 1095             


Asp Asp  Trp Asp Ala Glu Ala  Glu Val Glu Gly Ile  Arg Arg Ser 
    1100                 1105                 1110             


Leu Asn  Phe Arg Gln Cys Phe  Ile Ser Arg Met Pro  Phe Glu Asp 
    1115                 1120                 1125             


His Gly  Val Phe Trp Asp Ala  Thr Ile Tyr Ser Pro  Arg Ala Lys 
    1130                 1135                 1140             


Lys Thr  Ala Ala Leu Pro Leu  Lys Gln Gly Leu Asn  Pro Ser Arg 
    1145                 1150                 1155             


Tyr Gly  Ser Phe Ser Arg Glu  Gln Phe Ala Tyr Phe  Phe Ile Tyr 
    1160                 1165                 1170             


Lys Ala  Arg Asn Pro Arg Lys  Glu Gln Thr Leu Phe  Glu Phe Ala 
    1175                 1180                 1185             


Gln Val  Pro Val Arg Leu Ser  Ala Gln Ile Arg Gln  Asp Glu Asn 
    1190                 1195                 1200             


Ala Leu  Glu Arg Tyr Ala Arg  Glu Leu Ala Lys Asp  Gln Gly Leu 
    1205                 1210                 1215             


Glu Phe  Ile Arg Ile Glu Arg  Ser Lys Ile Leu Lys  Asn Gln Leu 
    1220                 1225                 1230             


Ile Glu  Ile Asp Gly Asp Arg  Leu Cys Ile Thr Gly  Lys Glu Glu 
    1235                 1240                 1245             


Val Arg  Asn Ala Cys Glu Leu  Ala Phe Ala Gln Asp  Glu Met Arg 
    1250                 1255                 1260             


Val Ile  Arg Met Leu Val Ser  Glu Lys Pro Val Ser  Arg Glu Cys 
    1265                 1270                 1275             


Val Ile  Ser Leu Phe Asn Arg  Ile Leu Leu His Gly  Asp Gln Ala 
    1280                 1285                 1290             


Ser Arg  Arg Leu Ser Lys Gln  Leu Lys Leu Ala Leu  Leu Ser Glu 
    1295                 1300                 1305             


Ala Phe  Ser Glu Ala Ser Asp  Asn Val Gln Arg Asn  Val Val Leu 
    1310                 1315                 1320             


Gly Leu  Ile Ala Ile Phe Asn  Gly Ser Thr Asn Met  Val Asn Leu 
    1325                 1330                 1335             


Ser Asp  Ile Gly Gly Ser Lys  Phe Ala Gly Asn Val  Arg Ile Lys 
    1340                 1345                 1350             


Tyr Lys  Lys Glu Leu Ala Ser  Pro Lys Val Asn Val  His Leu Ile 
    1355                 1360                 1365             


Asp Gln  Ser Val Thr Gly Met  Phe Glu Arg Arg Thr  Lys Ile Gly 
    1370                 1375                 1380             


Leu 
    


<210>  1110
<211>  1385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1110

Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         


Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Val Tyr 
        115                 120                 125             


His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ser Ser Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Phe Asn Ser 
                165                 170                 175     


Lys Asn Asn Asp Leu Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         


Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             


Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 


Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Arg Lys Tyr Phe 
                245                 250                 255     


Asn Leu Asp Glu Lys Thr Ser Leu Gln Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Thr Leu Leu Gly His Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             


Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Asn Gly Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 


Met Ile Met Arg Tyr Lys Glu His Glu Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     


Ala Tyr Ile Arg Asn Ile Ser Leu Glu Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         


Asp Asn Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             


Gln Glu Asp Phe Tyr Val Tyr Leu Lys Arg Leu Leu Ala Gly Leu Glu 
    370                 375                 380                 


Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile Tyr Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 


Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 


Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Asn Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             


Phe Ile Ala Glu Gly Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 


Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Gly Lys Arg Lys Val Thr 
545                 550                 555                 560 


Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Asp Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             


Ser Ser Asn Glu Thr Ile Ile Glu Glu Ile Ile His Thr Leu Thr Met 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Asp Asn 
625                 630                 635                 640 


Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         


Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Val Ser 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 


Lys Lys Ile Lys Lys Ala Gln Ile Ile Gly Asp Lys Asp Asn Ile Lys 
705                 710                 715                 720 


Gln Val Val Lys Ser Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         


Glu Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln Tyr Thr 
        755                 760                 765             


Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 


Ser Leu Lys Gly Leu Gly Ser Lys Ile Leu Lys Glu Asn Val Pro Thr 
785                 790                 795                 800 


Arg Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp Arg Leu Tyr 
                805                 810                 815     


Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu 
            820                 825                 830         


Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln 
        835                 840                 845             


Ala Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Val Ser Ser 
    850                 855                 860                 


Ala Ser Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Val Val 
865                 870                 875                 880 


Lys Lys Arg Lys Thr Leu Trp Tyr Gln Leu Leu Lys Ser Lys Leu Ile 
                885                 890                 895     


Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu 
            900                 905                 910         


Ser Gln Glu Glu Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr 
        915                 920                 925             


Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu Arg Phe Asn 
    930                 935                 940                 


Asn Lys Lys Asp Glu Asn Asn Arg Thr Leu Arg Thr Val Lys Ile Ile 
945                 950                 955                 960 


Thr Leu Lys Ser Ser Leu Val Ser Gln Phe Arg Lys Asp Phe Glu Leu 
                965                 970                 975     


Tyr Lys Val Arg Glu Ile Asn Asp Phe His His Ala His Asp Ala Tyr 
            980                 985                 990         


Leu Asn Ala Val Val Ala Ser Ala  Leu Leu Lys Lys Tyr  Pro Lys Leu 
        995                 1000                 1005             


Glu Pro  Glu Phe Val Tyr Gly  Asp Tyr Pro Lys Tyr  Asn Ser Phe 
    1010                 1015                 1020             


Arg Glu  Arg Lys Ser Ala Thr  Glu Lys Val Tyr Phe  Tyr Ser Asn 
    1025                 1030                 1035             


Ile Met  Asn Ile Phe Lys Lys  Ser Ile Pro Leu Ala  Asp Gly Thr 
    1040                 1045                 1050             


Val Ile  Asp Arg Pro Leu Ile  Glu Val Asn Glu Glu  Thr Gly Glu 
    1055                 1060                 1065             


Ser Val  Trp Asn Lys Val Ala  Asp Leu Asn Thr Val  Arg Lys Val 
    1070                 1075                 1080             


Leu Ser  Tyr Ser Gln Val Asn  Ile Val Lys Lys Val  Glu Glu Gln 
    1085                 1090                 1095             


Asn His  Gly Leu Asp Arg Gly  Lys Pro Lys Gly Leu  Phe Asn Ala 
    1100                 1105                 1110             


Asn Leu  Ser Ser Lys Pro Lys  Pro Asn Ser Lys Glu  Asn Leu Val 
    1115                 1120                 1125             


Gly Ala  Lys Glu Tyr Leu Asp  Pro Lys Lys Tyr Gly  Gly Tyr Ala 
    1130                 1135                 1140             


Gly Ile  Ser Asn Ser Phe Ala  Ile Leu Val Lys Gly  Thr Ile Glu 
    1145                 1150                 1155             


Lys Gly  Ala Lys Lys Lys Ile  Thr Asn Val Leu Glu  Phe Gln Gly 
    1160                 1165                 1170             


Ile Ser  Ile Leu Asp Arg Ile  Tyr Tyr Arg Lys Asp  Lys Leu Asn 
    1175                 1180                 1185             


Phe Leu  Leu Glu Lys Gly Tyr  Lys Asp Ile Glu Leu  Ile Ile Glu 
    1190                 1195                 1200             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Ser Asp Gly  Ser Arg Arg 
    1205                 1210                 1215             


Met Leu  Ala Ser Ile Leu Ser  Thr Asn Asn Lys Arg  Gly Glu Ile 
    1220                 1225                 1230             


His Lys  Gly Asn Gln Ile Phe  Ile Ser Gln Lys Phe  Val Lys Leu 
    1235                 1240                 1245             


Leu Tyr  His Ala Lys Arg Ile  Ser Ser Thr Phe Asn  Glu Asn His 
    1250                 1255                 1260             


Arg Lys  Tyr Val Glu Asn His  Lys Lys Glu Phe Glu  Glu Leu Phe 
    1265                 1270                 1275             


Tyr Tyr  Ile Leu Glu Phe Asn  Glu Asn Tyr Val Gly  Ala Lys Lys 
    1280                 1285                 1290             


Asn Gly  Glu Leu Leu Lys Ser  Ala Phe Gln Ser Trp  Gln Asn His 
    1295                 1300                 1305             


Ser Ile  Asp Glu Leu Cys Ser  Ser Phe Ile Gly Pro  Thr Gly Ser 
    1310                 1315                 1320             


Glu Arg  Lys Gly Leu Phe Glu  Leu Thr Ser Arg Gly  Gly Ala Ala 
    1325                 1330                 1335             


Asp Phe  Glu Phe Leu Gly Val  Lys Ile Pro Arg Tyr  Arg Asp Tyr 
    1340                 1345                 1350             


Thr Pro  Ser Ser Leu Leu Lys  Asp Ala Thr Leu Ile  His Gln Ser 
    1355                 1360                 1365             


Val Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1370                 1375                 1380             


Glu Asp  
    1385 


<210>  1111
<211>  1385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1111

Met Gln Thr Tyr Phe Gly Met Lys Lys Ile Leu Gly Leu Asp Leu Gly 
1               5                   10                  15      


Thr Asn Ser Ile Gly Trp Ala Leu Val Asn Glu Ala Asn Asp Asp Asn 
            20                  25                  30          


Glu Lys Ser Ser Ile Ile Lys Leu Gly Val Arg Val Asn Pro Leu Thr 
        35                  40                  45              


Val Asp Glu Gln Ser Asn Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn 
    50                  55                  60                  


Ala Glu Arg Thr Ile Lys Arg Ser Ile Arg Arg Ser Leu Gln Arg Tyr 
65                  70                  75                  80  


Lys Leu Arg Arg Glu Asn Leu Ile Glu Ile Leu Lys Glu His Asn Phe 
                85                  90                  95      


Ile Thr Asp Asp Thr Ile Leu Ser Glu Asp Gly Asn Ala Ser Thr Phe 
            100                 105                 110         


Glu Thr Tyr Arg Leu Arg Ala Lys Ala Ala Ser Glu Lys Ile Ser Leu 
        115                 120                 125             


Glu Glu Leu Gly Arg Val Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr 
    130                 135                 140                 


Lys Ser Ser Arg Lys Ala Lys Asn Gln Glu Glu Gly Gln Leu Ile Asp 
145                 150                 155                 160 


Gly Met Glu Val Ala Lys Glu Leu Tyr Glu Lys Asn Ile Thr Pro Gly 
                165                 170                 175     


Gln Phe Val Phe Gln Leu Leu Lys Gln Gly Gly Lys Phe Ile Pro Asp 
            180                 185                 190         


Phe Tyr Arg Ser Asp Leu Gln Ala Glu Phe Asp Lys Ile Trp Glu Phe 
        195                 200                 205             


Gln Lys Leu Phe His Ala Glu Ile Leu Val Asp Asp Phe Lys Lys Gln 
    210                 215                 220                 


Leu Glu Arg Lys Gly Lys Leu Asn Ser Ser Lys Ile Phe Leu Gly Arg 
225                 230                 235                 240 


Phe Gly Ile Tyr Thr Ala Glu Asn Lys Gly Asn Asp Lys Arg Leu Gln 
                245                 250                 255     


Ala Tyr Gln Trp Arg Val Asp Ala Leu Thr Lys Glu Leu Thr Lys Glu 
            260                 265                 270         


Glu Leu Ala Tyr Val Ile Ser Asp Leu Asn Gly Ile Ile Ser Ser Ser 
        275                 280                 285             


Ser Gly Tyr Leu Gly Ser Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe 
    290                 295                 300                 


Asn Asn Gln Thr Val Gly Gln Phe Leu Met Ala Gln Leu Asp His Ser 
305                 310                 315                 320 


Pro His Thr Ser Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu 
                325                 330                 335     


Asp Glu Phe Asn Thr Leu Trp Glu Asn Gln Ala His Tyr His Lys Val 
            340                 345                 350         


Leu Thr Ser Asp Leu Lys Ser Glu Ile Arg Asp Val Ile Ile Phe Tyr 
        355                 360                 365             


Gln Arg Arg Leu Lys Thr Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe 
    370                 375                 380                 


Glu Ser Arg Lys Ile Glu Val Glu Ile Asp Gly Lys Lys Lys Ile Lys 
385                 390                 395                 400 


Asn Val Gly Ser Arg Val Cys Pro Lys Ser Ser Pro Ile Phe Gln Glu 
                405                 410                 415     


Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Leu Leu Glu Lys Ser 
            420                 425                 430         


Ser Lys Ala Lys Arg Phe Leu Asp Gln Glu Glu Lys Glu Leu Leu Phe 
        435                 440                 445             


Lys Glu Leu Ser Ile Lys Ser Lys Ile Ser Lys Ser Asp Ala Leu Lys 
    450                 455                 460                 


Leu Leu Phe Lys Asn Ser Arg Glu Leu Asp Leu Asn Tyr Lys Glu Ile 
465                 470                 475                 480 


Glu Gly Asn Arg Thr Gln Ser Ala Leu Phe Asp Ala Tyr Ser Lys Ile 
                485                 490                 495     


Ile Glu Leu Ser Gly His Gly Glu Tyr Asp Phe Ser Lys Met Glu Ala 
            500                 505                 510         


Ser Asn Ile Ile Glu Leu Val Gln Glu Val Phe Thr Gly Leu Asn Tyr 
        515                 520                 525             


Asn Thr Glu Ile Leu Tyr Phe Asp Ser Ser Leu Glu Gly Lys Asp Phe 
    530                 535                 540                 


Glu Asn Gln Pro Met Tyr Arg Leu Trp His Leu Leu Tyr Ser Phe Glu 
545                 550                 555                 560 


Gly Asp Asp Ser Lys Thr Gly Asn Glu Lys Leu Ile Asn Lys Leu Ser 
                565                 570                 575     


Glu Leu Tyr Gly Phe Glu Lys Lys Tyr Ser Ile Val Leu Ala Asn Val 
            580                 585                 590         


Ser Phe Gln Glu Asp Tyr Gly Ser Leu Ser Thr Lys Ala Ile Arg Lys 
        595                 600                 605             


Ile His Pro Tyr Leu Lys Glu Gly Ile Gln Tyr Asp Val Ala Cys Glu 
    610                 615                 620                 


Phe Ala Gly Tyr Arg His Ser Lys Ser Ser Leu Thr Lys Glu Glu Ile 
625                 630                 635                 640 


Glu Asn Lys Ile Leu Lys Asp Gln Leu Glu Ile Leu Pro Lys Asn Ser 
                645                 650                 655     


Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Met Ile Asn Val 
            660                 665                 670         


Val Asn Ala Leu Asn Glu Ser Tyr Gly Lys Pro Asp Glu Ile Arg Ile 
        675                 680                 685             


Glu Leu Ala Arg Glu Leu Lys Lys Ser Ala Lys Glu Arg Glu Glu Leu 
    690                 695                 700                 


Thr Ala Ser Ile Gly Glu Thr Thr Ile Lys His Glu Lys Tyr Arg Ala 
705                 710                 715                 720 


Ile Leu Glu Asn Glu Phe Gly Ile Ser His Val Ser Arg Asn Asp Ile 
                725                 730                 735     


Ile Arg Tyr Lys Leu Tyr Leu Glu Leu Glu Asn Arg Gly Tyr Lys Thr 
            740                 745                 750         


Leu Tyr Ser Asn Thr Tyr Val Ser Gln Glu Lys Leu Phe Ser Lys Glu 
        755                 760                 765             


Phe Asp Ile Asp His Ile Ile Pro Gln Ser Cys Leu Phe Asp Asp Ser 
    770                 775                 780                 


Phe Ser Asn Lys Thr Leu Glu Ser Lys Ser Val Asn Ile Glu Lys Gly 
785                 790                 795                 800 


Asn Asp Thr Ala Phe Asp Tyr Val Phe Ala Lys Tyr Gly Glu Ala Gly 
                805                 810                 815     


Leu Ser Asp Tyr Ile Ile His Ile Glu Glu Leu Phe Lys Asp Gly Lys 
            820                 825                 830         


Ile Ser Lys Thr Lys Leu Asn Lys Leu Lys Met Lys Gly Asn Glu Ile 
        835                 840                 845             


Pro Ser Asp Phe Ile Ala Arg Asp Ile Arg Asp Thr Gln Tyr Ile Ala 
    850                 855                 860                 


Arg Ile Ala Lys Asn Ile Leu Glu Glu Thr Val Arg Asn Val Val Ser 
865                 870                 875                 880 


Thr Ser Gly Ser Ile Thr Asp Arg Leu Arg Glu Asp Trp Gln Leu Ile 
                885                 890                 895     


Asp Val Met Gln Glu Leu Asn Trp Glu Lys Tyr Ser Ala Leu Gly Leu 
            900                 905                 910         


Thr Glu Ile Tyr Glu Asn Arg Glu Gly His Thr Ile Arg Arg Ile Lys 
        915                 920                 925             


Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu 
    930                 935                 940                 


Thr Ile Ala Phe Thr Lys Arg Ser His Ile Gln Tyr Leu Asn Asn Leu 
945                 950                 955                 960 


Asn Ala Arg Ser Asp Lys Ser Gly Ser Ile Tyr Gly Ile Glu Thr Lys 
                965                 970                 975     


Glu Leu Tyr Arg Asp Asp His Gly Lys Leu Lys Phe Lys Pro Pro Ile 
            980                 985                 990         


Pro Leu Asn Glu Phe Arg Ala Glu  Ala Lys Lys His Leu  Glu Asn Thr 
        995                 1000                 1005             


Leu Ile  Ser Ile Lys Ala Lys  Asn Lys Val Ala Thr  Ile Asn Ile 
    1010                 1015                 1020             


Asn Thr  Thr Ser Thr Lys Glu  Gly Lys Asn Lys Lys  Arg Gln Leu 
    1025                 1030                 1035             


Thr Pro  Arg Gly Gln Leu His  Leu Glu Thr Ile Tyr  Gly Ser Ile 
    1040                 1045                 1050             


Gln Gln  Tyr Val Thr Lys Gln  Glu Lys Val Gly Ala  Ser Phe Asn 
    1055                 1060                 1065             


Glu Glu  Gln Ile Ser Lys Val  Ala Lys Gln Lys Tyr  Arg Gln Ala 
    1070                 1075                 1080             


Leu Leu  Asn Arg Leu Arg Glu  Phe Asp Asn Asp Pro  Lys Lys Ala 
    1085                 1090                 1095             


Phe Thr  Gly Asn Asn Ser Phe  Glu Lys Asn Pro Ile  Tyr Leu Asp 
    1100                 1105                 1110             


Val Leu  Gln Thr Gln Lys Val  Pro Asp Lys Val Glu  Thr Val Ser 
    1115                 1120                 1125             


Lys Glu  Ile Ile Tyr Thr Ile  Arg Lys Asp Ile Thr  Pro Glu Leu 
    1130                 1135                 1140             


Lys Ile  Glu Lys Val Ile Asp  Lys Lys Ile Lys Thr  Ile Leu Gln 
    1145                 1150                 1155             


Ser Arg  Leu Asn Glu Tyr Ser  Gly Asp Ser Lys Arg  Ala Phe Ser 
    1160                 1165                 1170             


Asn Leu  Lys Glu Asn Pro Ile  Trp Leu Asn Gln Glu  Lys Gly Ile 
    1175                 1180                 1185             


Glu Ile  Lys Arg Val Thr Ile  Ser Gly Ile Ser Asn  Ala Val Ala 
    1190                 1195                 1200             


Ile His  Asp Lys Lys Asp Lys  Asn Gly Asn Leu Ile  Leu Asp Asn 
    1205                 1210                 1215             


Glu Gly  Ser Thr Gln Ala Val  Asp Phe Val Asn Thr  Gly Asn Asn 
    1220                 1225                 1230             


His His  Val Ala Ile Tyr Lys  Asp Glu Arg Gly Asn  Leu His Glu 
    1235                 1240                 1245             


Ser Val  Val Ser Phe Phe Glu  Ala Cys Thr Arg Val  Asn Leu Gly 
    1250                 1255                 1260             


Gly Pro  Val Ile Phe Lys Asn  Tyr Asn Lys Ser Asp  Gly Trp Glu 
    1265                 1270                 1275             


Phe Leu  Phe Ser Met Lys Gln  Asn Glu Tyr Phe Val  Phe Pro Asn 
    1280                 1285                 1290             


Glu Lys  Thr Gly Phe Asn Pro  Asn Glu Ile Asp Leu  Leu Asp Pro 
    1295                 1300                 1305             


Asp Asn  Tyr Arg Leu Ile Ser  Pro Asn Leu Phe Arg  Val Gln Lys 
    1310                 1315                 1320             


Ile Ser  Ser Lys Asn Tyr Val  Phe Asn His His Leu  Glu Thr Ser 
    1325                 1330                 1335             


Ala Ile  Thr Gly Glu Thr Leu  Lys Asn Lys Lys Gln  Leu Ser Ser 
    1340                 1345                 1350             


Val Thr  Tyr His Phe Ile Gln  Ser Leu Pro Pro Ile  Lys Asp Ile 
    1355                 1360                 1365             


Val Lys  Val Arg Ile Asn Asn  Ile Gly Gln Ile Val  Trp Val Gly 
    1370                 1375                 1380             


Glu Tyr  
    1385 


<210>  1112
<211>  1385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1112

Met Asp Glu Leu Gln Asn Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn 
1               5                   10                  15      


Ala Asp Arg Thr Ala Gly Arg Ser Met Arg Arg Asn Leu Gln Arg Tyr 
            20                  25                  30          


Lys Leu Arg Arg Glu Asn Leu Ile Glu Val Leu Lys Glu Tyr Asn Phe 
        35                  40                  45              


Ile Thr Asp Glu Met Ile Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe 
    50                  55                  60                  


Glu Thr Tyr Arg Leu Arg Ala Lys Ala Ala Val Glu Glu Ile Ser Leu 
65                  70                  75                  80  


Glu Gln Phe Ala Arg Val Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr 
                85                  90                  95      


Lys Ser Ser Arg Lys Ala Lys Gly Ala Glu Asn Gly Thr Leu Ile Asp 
            100                 105                 110         


Gly Met Glu Val Ala Lys Lys Met Tyr Asp Glu Gly Ile Thr Pro Gly 
        115                 120                 125             


Glu Leu Cys Leu Gln Leu Leu Thr Ala Gly Lys Arg Tyr Phe Pro Asp 
    130                 135                 140                 


Phe Tyr Arg Ser Asp Leu Gln Ala Glu Phe Asp Arg Ile Trp Asn Phe 
145                 150                 155                 160 


Gln Lys Gln Phe Tyr Pro Asp Ser Leu Ile Asp Lys Val Lys Asp Glu 
                165                 170                 175     


Val Arg Gly Lys Asn Lys Ser Gln Thr Trp Ala Ile Leu Ala Lys Tyr 
            180                 185                 190         


Phe Val Trp Lys Glu Val Glu Asn Ser Trp Asn Glu Glu Glu Ala Gln 
        195                 200                 205             


Thr Arg Arg Val Glu Lys Glu Tyr Arg Leu Val Gly Ile Lys Arg Gly 
    210                 215                 220                 


Val Lys Arg Glu Glu Leu Lys Leu Glu Asn Phe Gln Trp Arg Val Lys 
225                 230                 235                 240 


Ala Leu Ser Glu Arg Met Asn Pro Glu Glu Leu Ala Ile Val Leu Gln 
                245                 250                 255     


Glu Ile Asn Glu Gln Ile Ser Asn Ser Ser Gly Tyr Leu Gly Ala Ile 
            260                 265                 270         


Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Arg Gln Thr Val Gly Gln 
        275                 280                 285             


Tyr Gln Met Ala Val Leu Asp Asn Asn Pro Asn Ile Gly Leu Arg Asn 
    290                 295                 300                 


Met Val Phe Tyr Arg Gln Gly Tyr Leu Gly Glu Phe Asn Thr Ile Trp 
305                 310                 315                 320 


Glu Lys Gln Ala Glu Phe His Lys Glu Leu Thr Glu Asp Leu Lys Lys 
                325                 330                 335     


Glu Ile Arg Asp Ile Val Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln 
            340                 345                 350         


Lys Gly Leu Ile Asn Ile Cys Glu Phe Glu Arg Arg Gln Ile Glu Val 
        355                 360                 365             


Glu Ile Asp Gly Lys Lys Lys Ile Lys Thr Ile Gly Ser Arg Val Ile 
    370                 375                 380                 


Pro Arg Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Thr Leu 
385                 390                 395                 400 


Asn Asp Ile Glu Val Ser Val Leu Gly Val Lys Asn Lys Arg Lys Lys 
                405                 410                 415     


Gln Asp Asp Asn Ser Thr Thr Leu Leu Asp Ser Ala Glu Asn Ile Asp 
            420                 425                 430         


Ser Leu Lys Leu Asn Val Ser Arg Pro Leu Asp Ala Asp Glu Lys Ser 
        435                 440                 445             


Leu Leu Ala Lys Glu Leu Phe Ile Arg Asp Lys Leu Thr Lys Ser Asp 
    450                 455                 460                 


Val Leu Lys Leu Leu Phe Asn Asn Pro Gln Asn Leu Asn Leu Asn Phe 
465                 470                 475                 480 


Lys Asn Ile Asp Gly Asn Arg Thr Gly Ser Ala Leu Tyr Gln Ala Phe 
                485                 490                 495     


Ser Lys Ile Leu Glu Ile Ser Gly His Glu Ser Ile Asn Phe Lys Lys 
            500                 505                 510         


Ser Ala Asp Glu Ile Val Glu Gln Val Lys Thr Ile Phe Ser Ala Leu 
        515                 520                 525             


Gly Trp Asn Thr Glu Val Leu Cys Phe Asp Ser Glu Lys Glu Leu Asp 
    530                 535                 540                 


Lys Gln Pro Tyr Phe Lys Leu Trp His Leu Leu Tyr Ser Phe Glu Gly 
545                 550                 555                 560 


Asp Ser Thr Pro Thr Gly Asp Gly Asn Leu Ile Gln Lys Ile Ala Asp 
                565                 570                 575     


Leu Cys Gly Phe Glu Lys Asp Tyr Ala Ser Ile Leu Ala Asn Ile Thr 
            580                 585                 590         


Phe Gln Glu Asp Tyr Gly Ser Leu Ser Thr Lys Ala Ile Arg Lys Ile 
        595                 600                 605             


Leu Pro His Leu Lys Asp Gly Asn Gln Tyr Asp Val Ala Cys Glu Tyr 
    610                 615                 620                 


Ala Gly Tyr Lys His Ser Lys Ser Ser Leu Lys Lys Glu Glu Ile Lys 
625                 630                 635                 640 


Asn Lys Val Leu Lys Asp Lys Leu Glu Leu Leu Pro Lys Asn Ser Leu 
                645                 650                 655     


Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Met Val Asn Val Ile 
            660                 665                 670         


Asn Ala Ile Ile Thr Thr Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu 
        675                 680                 685             


Leu Ala Arg Glu Leu Lys Lys Lys Ala Lys Lys Arg Glu Glu Leu Thr 
    690                 695                 700                 


Lys Thr Ile Val Asp Thr Thr Lys Thr His Glu Gly Ile Arg Ala Ile 
705                 710                 715                 720 


Leu Gln Asn Asp Phe Gly Met Ser His Val Ser Arg Asn Ala Ile Ile 
                725                 730                 735     


Arg Tyr Lys Leu Tyr Glu Glu Leu Lys Asp Asn Gly Tyr Lys Thr Leu 
            740                 745                 750         


Tyr Ser Asn Thr Tyr Ile Pro Lys Glu Lys Leu Phe Ser Lys Asp Phe 
        755                 760                 765             


Asp Ile Glu His Ile Ile Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe 
    770                 775                 780                 


Ser Asn Lys Thr Leu Glu Val Lys Ser Ile Asn Ile Glu Lys Gly Ser 
785                 790                 795                 800 


Lys Thr Ala Tyr Asp Phe Val Glu Glu Lys Tyr Gly Glu Gln Gly Leu 
                805                 810                 815     


Gln Glu Tyr Leu Asn Arg Cys Glu Val Leu Phe Arg Asp Lys Lys Thr 
            820                 825                 830         


Lys Leu Arg Lys Leu Lys Met Gln Glu Ser Glu Ile Pro Glu Gly Phe 
        835                 840                 845             


Ile Asp Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala Leu 
    850                 855                 860                 


Ser Met Leu Asn Glu Ile Cys Arg Arg Val Val Ala Thr Thr Gly Ala 
865                 870                 875                 880 


Ile Thr Asp Glu Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Lys 
                885                 890                 895     


Glu Leu Asn Trp Glu Lys Tyr Lys Val Leu Gly Leu Val Glu Tyr Phe 
            900                 905                 910         


Glu Asp Asn Asp Gly Arg Leu Ile Gly Arg Ile Lys Asp Trp Thr Lys 
        915                 920                 925             


Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala Phe 
    930                 935                 940                 


Thr Lys Asp Val Phe Ile Gln Tyr Phe Asn Asn Lys Asn Ala Ser Leu 
945                 950                 955                 960 


Lys Pro Asp Thr Asn Glu Ala Ala Ile Arg Asn Lys Tyr Phe Gln Asn 
                965                 970                 975     


Gly Lys Ala Ile Pro Pro Met Ser Leu Asp Glu Phe Arg Ala Glu Ala 
            980                 985                 990         


Lys Lys His Leu Asn Asp Thr Leu  Ile Ser Ile Lys Thr  Lys Asn Lys 
        995                 1000                 1005             


Val Val  Thr Ile Asn Leu Asn  Arg Thr Lys Lys Lys  Asn Gly Ile 
    1010                 1015                 1020             


Asn Lys  Lys Gln Gln Gln Thr  Pro Arg Gly Gln Leu  His Leu Glu 
    1025                 1030                 1035             


Thr Val  Tyr Gly Ser His Lys  Gln Tyr Val Val Lys  Glu Glu Lys 
    1040                 1045                 1050             


Val Asn  Ala Ser Phe Asp Ala  Ala Lys Ile Ala Thr  Val Asn Lys 
    1055                 1060                 1065             


Pro Ala  Tyr Arg Asp Ala Leu  Leu Arg Arg Leu Gln  Glu Asn Gly 
    1070                 1075                 1080             


Asn Asp  Ser Lys Lys Ala Phe  Thr Gly Lys Asn Ser  Leu Asp Lys 
    1085                 1090                 1095             


Gln Pro  Val Trp Leu Asp Ala  Lys Gln Asn Glu Cys  Val Pro Glu 
    1100                 1105                 1110             


Lys Val  Lys Thr Val Met Leu  Glu Thr Val Tyr Thr  Val Arg Lys 
    1115                 1120                 1125             


Pro Ile  Asp Glu Lys Leu Asn  Val Asp Lys Val Val  Asp Ala Arg 
    1130                 1135                 1140             


Ile Arg  Lys Leu Leu Gln Asp  Arg Ile Lys Glu Tyr  Gly Asn Ala 
    1145                 1150                 1155             


Lys Ser  Ala Phe Ala Asn Leu  Asp Glu Asn Pro Ile  Trp Leu Asn 
    1160                 1165                 1170             


Lys Glu  Lys Gly Ile Thr Ile  Lys Arg Val Ser Val  Ser Gly Ile 
    1175                 1180                 1185             


Ser Lys  Ala Gln Ser Leu His  Glu Lys Lys Asp Lys  Asp Gly Asn 
    1190                 1195                 1200             


Phe Ile  Leu Asp Lys Asp Ser  Arg Arg Ile Pro Val  Asp Phe Val 
    1205                 1210                 1215             


Asn Thr  Gly Asn Asn His His  Val Ala Ile Tyr Arg  Lys Pro Val 
    1220                 1225                 1230             


Leu Asp  Lys Lys Gly Gln Ala  Val Phe Asp Glu Asn  Gly Glu Leu 
    1235                 1240                 1245             


Lys Phe  Glu Leu Glu Glu Val  Val Val Ser Phe Phe  Asp Ala Val 
    1250                 1255                 1260             


Thr Arg  Val Asn Leu Ser Gln  Pro Ile Ile Asp Lys  Asp Tyr Lys 
    1265                 1270                 1275             


Arg Ser  Glu Gly Trp Lys Phe  Leu Phe Ser Met Lys  Gln Asn Glu 
    1280                 1285                 1290             


Tyr Phe  Val Phe Pro Asn Glu  Lys Thr Gly Phe Asp  Pro Lys Glu 
    1295                 1300                 1305             


Ile Asp  Leu Leu Asn Pro Glu  Asn Tyr Ala Leu Ile  Ser Pro Asn 
    1310                 1315                 1320             


Leu Phe  Arg Val Gln Lys Ile  Ala Ser Lys Asp Tyr  Val Phe Arg 
    1325                 1330                 1335             


His His  Leu Glu Thr Val Val  Ala Asp Arg Lys Glu  Leu Gln Glu 
    1340                 1345                 1350             


Ile Thr  Trp Lys Arg Ile Ser  Ser Leu Ser Ile Leu  Asn Arg Ile 
    1355                 1360                 1365             


Ile Lys  Val Arg Val Asn His  Ile Gly Gln Ile Val  Met Ile Gly 
    1370                 1375                 1380             


Glu Tyr  
    1385 


<210>  1113
<211>  1385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1113

Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         


Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             


His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 


Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         


Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             


Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 


Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr Phe 
                245                 250                 255     


Asn Leu Asp Glu Lys Ala Pro Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             


Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Asn Gly Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 


Met Ile Met Arg Tyr Lys Glu His Glu Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     


Ala Tyr Ile Arg Asn Ile Ser Leu Glu Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         


Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             


Gln Glu Asp Phe Tyr Val His Leu Lys Lys Leu Leu Val Gly Leu Glu 
    370                 375                 380                 


Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 


Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 


Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             


Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 


Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asn Lys Arg Arg Val Thr 
545                 550                 555                 560 


Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Asp Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             


Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
625                 630                 635                 640 


Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         


Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Val Ser 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 


Lys Lys Ile Lys Lys Ala Gln Ile Ile Gly Asp Lys Asp Asn Ile Lys 
705                 710                 715                 720 


Gln Val Val Gln Ala Leu Pro Gly Arg Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         


Arg Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln Tyr Thr 
        755                 760                 765             


Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 


Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn Ile Pro Ala 
785                 790                 795                 800 


Lys Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp Arg Leu Tyr 
                805                 810                 815     


Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu 
            820                 825                 830         


Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln 
        835                 840                 845             


Ala Phe Leu Lys Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser 
    850                 855                 860                 


Ala Ser Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Val Val 
865                 870                 875                 880 


Lys Lys Arg Lys Thr Leu Trp Tyr Gln Leu Leu Lys Ser Lys Leu Ile 
                885                 890                 895     


Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu 
            900                 905                 910         


Ser Pro Glu Ala Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr 
        915                 920                 925             


Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu Arg Phe Asn 
    930                 935                 940                 


Asn Lys Lys Asp Glu Asp Asn Lys Thr Leu Arg Thr Val Lys Ile Ile 
945                 950                 955                 960 


Thr Leu Lys Ser Ser Leu Val Ser Gln Phe Arg Lys Asp Phe Glu Leu 
                965                 970                 975     


Tyr Lys Val Arg Glu Ile Asn Asp Phe His His Ala His Asp Ala Tyr 
            980                 985                 990         


Leu Asn Ala Val Val Ala Ser Ala  Leu Leu Lys Lys Tyr  Pro Lys Leu 
        995                 1000                 1005             


Glu Pro  Glu Phe Val Tyr Gly  Asp Tyr Pro Lys Tyr  Asn Ser Phe 
    1010                 1015                 1020             


Arg Glu  Arg Lys Ser Ala Thr  Glu Lys Val Tyr Phe  Tyr Ser Asn 
    1025                 1030                 1035             


Ile Met  Asn Ile Phe Lys Lys  Ser Ile Ser Leu Ala  Asp Gly Thr 
    1040                 1045                 1050             


Val Ile  Glu Arg Pro Leu Ile  Glu Val Asn Glu Glu  Thr Gly Glu 
    1055                 1060                 1065             


Ser Val  Trp Asn Lys Val Ala  Asp Leu Asn Thr Val  Arg Lys Val 
    1070                 1075                 1080             


Leu Ser  Tyr Ser Gln Val Asn  Ile Val Lys Lys Val  Glu Glu Gln 
    1085                 1090                 1095             


Asn His  Gly Leu Asp Arg Gly  Lys Pro Lys Gly Leu  Phe Asn Ala 
    1100                 1105                 1110             


Asn Leu  Ser Ser Lys Pro Lys  Pro Asn Ser Lys Glu  Asn Leu Val 
    1115                 1120                 1125             


Gly Ala  Lys Glu Tyr Leu Asp  Pro Lys Lys Tyr Gly  Gly Tyr Ala 
    1130                 1135                 1140             


Gly Ile  Ser Asn Ser Phe Ala  Val Leu Val Lys Gly  Thr Ile Glu 
    1145                 1150                 1155             


Lys Gly  Ala Lys Lys Lys Ile  Thr Asn Ile Leu Glu  Phe Gln Gly 
    1160                 1165                 1170             


Ile Ser  Ile Leu Asp Arg Ile  Thr Tyr Arg Lys Asp  Lys Phe Asn 
    1175                 1180                 1185             


Phe Leu  Leu Glu Lys Gly Tyr  Lys Asp Ile Asp Leu  Ile Ile Glu 
    1190                 1195                 1200             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Ser Asp Gly  Ser Arg Arg 
    1205                 1210                 1215             


Met Leu  Ala Ser Ile Leu Ser  Thr Asn Asn Lys Arg  Gly Glu Ile 
    1220                 1225                 1230             


His Lys  Gly Asn Gln Ile Phe  Ile Ser Gln Lys Tyr  Val Lys Leu 
    1235                 1240                 1245             


Leu Tyr  His Ala Lys Arg Ile  Ser Asn Thr Phe Asn  Asp Asn His 
    1250                 1255                 1260             


Arg Lys  Tyr Val Glu Asn His  Lys Lys Glu Phe Glu  Glu Leu Phe 
    1265                 1270                 1275             


Tyr Tyr  Ile Leu Glu Phe Asn  Glu Asn Tyr Val Gly  Ala Lys Lys 
    1280                 1285                 1290             


Asn Gly  Glu Leu Leu Lys Ser  Ala Phe Gln Ser Trp  Gln Asn His 
    1295                 1300                 1305             


Ser Ile  Asp Glu Leu Cys Ser  Ser Phe Ile Gly Pro  Thr Gly Ser 
    1310                 1315                 1320             


Glu Arg  Lys Gly Leu Phe Glu  Leu Thr Ser Arg Gly  Gly Ala Ala 
    1325                 1330                 1335             


Asp Phe  Asp Phe Leu Gly Val  Lys Ile Pro Arg Tyr  Arg Asp Tyr 
    1340                 1345                 1350             


Thr Pro  Ser Ser Leu Leu Lys  Asp Ala Thr Leu Ile  His Gln Ser 
    1355                 1360                 1365             


Val Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Gly  Lys Leu Gly 
    1370                 1375                 1380             


Glu Asp  
    1385 


<210>  1114
<211>  1385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1114

Met Lys Asn Lys Thr Trp Tyr Leu Gly Leu Gly Val Gly Pro Thr Ser 
1               5                   10                  15      


Ile Gly Trp Ala Ala Thr Asp Ser Asn Tyr Lys Leu Leu Arg Lys Asn 
            20                  25                  30          


Lys Lys Arg Leu Trp Gly Val Arg Leu Phe Glu Glu Ala Lys Thr Ala 
        35                  40                  45              


Val Asp Arg Arg Thr Phe Arg Ala Glu Arg Arg Arg Leu Ala Arg Arg 
    50                  55                  60                  


Lys Trp Arg Ile Ser Leu Leu Glu Glu Leu Phe Ala Lys Glu Val Ser 
65                  70                  75                  80  


Lys Asn Asp Pro Asn Phe Phe Leu Arg Leu Lys Glu Ser Lys Tyr His 
                85                  90                  95      


Phe Glu Asp Lys Ser Leu Met Asn Glu Tyr Ile Leu Phe Asn Asp Lys 
            100                 105                 110         


Asp Tyr Thr Asp Lys Asp Tyr Tyr Lys Glu Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Leu Ser Leu Thr Thr Glu Glu Asn Pro Asp Ile Arg Lys Val 
    130                 135                 140                 


Tyr Leu Ala Val His Ser Met Leu Lys Asn Arg Gly His Phe Leu Leu 
145                 150                 155                 160 


Gln Gly Gln Ser Phe Asn Asp Ser Asn Ile Asp Ser Val Ile Lys Glu 
                165                 170                 175     


Leu Leu Glu Leu Glu Thr Val Lys Ile Asn Phe Glu Val Thr Asp Glu 
            180                 185                 190         


Val Ile His Ser Leu Lys Lys Ile Ala Leu Glu Lys Lys Asn Leu Ser 
        195                 200                 205             


Asp Lys Asn Asn Asp Val Lys Lys Leu Tyr Pro Lys Glu Lys Gln Leu 
    210                 215                 220                 


Gln Glu Ile Phe Lys Leu Val Phe Gly Gly Lys Thr Ser Leu Asp Lys 
225                 230                 235                 240 


Leu Tyr Asp Ile Glu Glu Tyr Lys Glu Leu Asp Asn Ser Val Lys Ser 
                245                 250                 255     


Ile Ser Phe Lys Glu Lys Val Tyr Glu Glu Val Arg Pro Asp Tyr Glu 
            260                 265                 270         


Gln Ile Leu Ser Asn Tyr Ile Glu Leu Leu Asp Leu Ala Lys Leu Val 
        275                 280                 285             


Tyr Asp Ser Ile Val Leu Ala Asp Ile Lys Lys Glu Gly Lys Thr Ile 
    290                 295                 300                 


Ser Glu Ser Lys Val Glu Leu Tyr Asn Lys His Lys Asp Asp Leu Ala 
305                 310                 315                 320 


Asn Leu Lys Lys Leu Val Lys Ile Asp Ser Lys Leu Ser Glu Asp Lys 
                325                 330                 335     


Lys Lys Glu Val Tyr Phe Thr Ile Phe Lys Glu Asp Lys Glu Lys Gly 
            340                 345                 350         


Thr Asn Tyr Val Asn Tyr Thr His Arg Ser Gln Glu Gly Lys Ser Cys 
        355                 360                 365             


Ser Tyr Glu Asp Phe Lys Lys Phe Leu Lys Lys Glu Leu Glu Lys Leu 
    370                 375                 380                 


Glu Asp Ser Pro Thr Lys Thr Thr Ile Leu Glu Glu Leu Asp Leu Glu 
385                 390                 395                 400 


Thr Phe Leu Pro Leu Gln Arg Thr Lys Asp Asn Ser Val Val Pro Tyr 
                405                 410                 415     


Gln Ile His Lys Glu Glu Leu Val Lys Ile Leu Asp Asn Ala Ala Lys 
            420                 425                 430         


Tyr His Ser Phe Leu Ala Glu Lys Asp Asp Ser Gly Tyr Ser Thr Arg 
        435                 440                 445             


Glu Lys Val Val Gln Leu Phe Glu Phe Arg Ile Pro Tyr Tyr Ile Gly 
    450                 455                 460                 


Pro Leu Asn Ala His His Asn Thr Glu Asn Gly Gly Tyr Ser Trp Ala 
465                 470                 475                 480 


Ile Arg Lys Lys Gly Met Glu Ser Val Pro Val Thr Pro Trp Asn Phe 
                485                 490                 495     


Lys Asp Val Ile Asp Glu Ser Ala Ser Ala Glu Ala Phe Val Asn Asn 
            500                 505                 510         


Leu Lys Asn Lys Cys Ala Tyr Leu Ser Gly Glu Asp Val Leu Pro Lys 
        515                 520                 525             


Asn Ser Leu Leu Tyr Ser Glu Tyr Ala Leu Leu Asn Glu Leu Asn Ala 
    530                 535                 540                 


Leu Arg Tyr Glu Gly Asn Arg Ile Ser Val Glu Ala Arg Asp Val Ile 
545                 550                 555                 560 


Val Thr Lys Leu Phe Lys Glu Gln Gly Lys Lys Val Thr Lys Lys Thr 
                565                 570                 575     


Ile Lys Glu Leu Leu Lys Thr Glu Gly Phe Ile Asp Gly Lys Gly Glu 
            580                 585                 590         


Ile Val Gly Ile Asp Asn Ile Val Lys Asn Asp Leu Lys Ser Tyr Arg 
        595                 600                 605             


Asp Phe Lys Lys Ile Leu Ala Asp Lys Phe Asp Lys Glu Arg Val Glu 
    610                 615                 620                 


Asn Ile Ile Leu Trp Ile Thr Leu Tyr Gly Glu Ser Arg Lys Leu Ile 
625                 630                 635                 640 


Lys Glu Lys Ile Thr Lys Val Tyr Gly Asp Phe Tyr Thr Val Asp Glu 
                645                 650                 655     


Ile Glu Lys Met Ser Arg Leu Thr Tyr Lys Asp Trp Gly Ile Leu Ser 
            660                 665                 670         


Arg Lys Leu Leu Thr Glu Leu Val Ser Glu Lys Leu Tyr Asn Glu Glu 
        675                 680                 685             


Thr Gly Glu Cys Leu Asn Ile Ile Gly Ala Met Arg Gln Gly Thr Ile 
    690                 695                 700                 


Leu Phe Met Glu Leu Leu Ala Asp Lys Phe Asp Tyr Met Ser Gln Ile 
705                 710                 715                 720 


Lys Asn His Asn Lys Glu Asn Lys Asp Asp Ile Thr Glu Ile Thr Pro 
                725                 730                 735     


Asp Ile Leu Glu Asn Leu His Ala Ser Pro Ala Val Lys Arg Ser Ile 
            740                 745                 750         


Trp Gln Thr Val Arg Ile Val Glu Glu Leu Lys Thr Ile Ile Gly Cys 
        755                 760                 765             


Val Pro Thr Lys Ile Phe Ile Glu Thr Thr Lys Arg Asn Asn Ala Pro 
    770                 775                 780                 


Tyr Lys Val Pro Thr Leu Asn Arg Asn Asp Leu Val Thr Lys Tyr Lys 
785                 790                 795                 800 


Thr Ile Arg Glu His Glu Ile Phe Glu Leu Glu Lys Glu Leu Lys Asn 
                805                 810                 815     


Thr Leu Asp Phe Thr Leu Asn Lys Asn Arg Leu Ile Asn Glu Glu Pro 
            820                 825                 830         


Ser Lys Leu Lys Ala Thr Lys Leu Tyr Leu Tyr Tyr Thr Gln Leu Gly 
        835                 840                 845             


Arg Cys Met Tyr Thr Gly Lys Lys Ile Asp Leu Gly Glu Leu Phe Asp 
    850                 855                 860                 


Asn Asn Asn Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val 
865                 870                 875                 880 


Lys Asp Gly Ser Phe Asn Asn Ile Val Leu Val Thr Lys Ala Ser Asn 
                885                 890                 895     


Asp Met Lys Ala Asp Val Phe Pro Leu Lys Ser Lys Phe Gln Thr Lys 
            900                 905                 910         


Glu Asn Lys Ile Phe Trp Arg Phe Leu Lys Glu Lys Lys Leu Ile Thr 
        915                 920                 925             


Glu Glu Lys Tyr Asn Arg Leu Val Arg Thr Glu Glu Phe Ser Asn Gly 
    930                 935                 940                 


Glu Leu Thr Gly Phe Ile Thr Arg Gln Leu Val Glu Thr Ser Gln Ala 
945                 950                 955                 960 


Ile Lys Val Ile Ser Asn Val Leu Arg Glu Leu Asn Pro Glu Thr Thr 
                965                 970                 975     


Ile Cys Tyr Ser Lys Ala Glu Asn Val Ser Ser Phe Arg Gln Ser Phe 
            980                 985                 990         


Gly Lys Ile Lys Glu Ala Asn Arg  Lys Ala Glu Asn Asn  Glu Lys Leu 
        995                 1000                 1005             


Ile Arg  Val Gln Glu Ile Asn  Asp Tyr Tyr Asn Ala  Lys Asp Ala 
    1010                 1015                 1020             


Tyr Leu  Ser Ile Val Val Gly  Asn Val Tyr Asp Val  Lys Phe Thr 
    1025                 1030                 1035             


Arg Asn  Val His Asn Phe Ile  Lys Asn Asn Lys Asp  Ala Arg Arg 
    1040                 1045                 1050             


Tyr Ser  Leu Asn Gly Leu Phe  Tyr Thr Asp Val Lys  Asn Ala Asn 
    1055                 1060                 1065             


Thr Ile  Ala Trp Glu Met Asp  Lys Thr Val His Val  Val Glu Lys 
    1070                 1075                 1080             


Thr Met  Asn Asn Asn Asn Ile  Leu Ile Thr Arg Arg  Thr Ser Glu 
    1085                 1090                 1095             


Gln Lys  Gly Ala Leu Tyr Asp  Ala Thr Leu Tyr Lys  Ala Lys Val 
    1100                 1105                 1110             


Ala Glu  Lys Ala Lys Glu Gly  Thr Tyr Tyr Pro Leu  Lys Thr Thr 
    1115                 1120                 1125             


Asp Ser  Lys Ile Lys Asp Val  Gly Lys Tyr Gly Gly  Phe Thr Lys 
    1130                 1135                 1140             


Ile Lys  Ile Ala Tyr Tyr Ser  Ile Phe Glu Tyr Thr  Leu Asp Gly 
    1145                 1150                 1155             


Lys Lys  Gly Lys Gln Lys Ile  Thr Arg Ile Val Pro  Ile Pro Ile 
    1160                 1165                 1170             


Tyr Ile  Ser Gln Asn Ile Lys  Asp Asp Lys Ala Leu  Leu Glu Tyr 
    1175                 1180                 1185             


Ala Gln  Thr Arg Leu Pro Gly  Lys Asp Leu Lys Ile  Lys Tyr Arg 
    1190                 1195                 1200             


Lys Leu  Cys Ile Gly Ser Leu  Val Lys Ile Asn Asn  Phe Asn Tyr 
    1205                 1210                 1215             


Tyr Ile  Gly Gly Lys Thr Asn  Asn Asn Phe Thr Phe  Asp Ser Ala 
    1220                 1225                 1230             


Val Gln  Val Ile Leu Asp Lys  Asp Ser Glu Ser Tyr  Ile Lys Glu 
    1235                 1240                 1245             


Leu Ala  Lys Tyr Leu Asn Trp  Lys Lys Glu Asn Lys  Asp Gly Lys 
    1250                 1255                 1260             


Leu Trp  Glu Ser Ile Thr Glu  Asp Lys Asn Ile Gln  Leu Tyr Asp 
    1265                 1270                 1275             


Ser Leu  Val Lys Lys Met Asn  Thr Gly Ile Phe Ile  Arg Lys Thr 
    1280                 1285                 1290             


Pro Asn  Lys Tyr Thr Glu Leu  Leu Thr Leu Glu Val  Lys Asn Lys 
    1295                 1300                 1305             


Phe Ile  Asn Ile Asp Ile Asp  Glu Gln Val Lys Leu  Leu Leu Glu 
    1310                 1315                 1320             


Ile Leu  Asn Leu Leu Thr Asn  Lys Lys Phe Thr Ser  Lys Leu Asn 
    1325                 1330                 1335             


Glu Ile  Gly Ile Thr Ala Ser  Arg Gly Val Cys Ser  Phe Asn Leu 
    1340                 1345                 1350             


Ser Asn  Gln Ser Gln Phe Ser  Ile Ile Glu Gln Ser  Val Thr Gly 
    1355                 1360                 1365             


Val Tyr  Glu Lys Glu Lys Leu  Ile Ile Glu Lys Lys  Lys Glu Gly 
    1370                 1375                 1380             


Ile Leu  
    1385 


<210>  1115
<211>  1386
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1115

Met Asn Gln Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Met Gln Val Asp Glu Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         


Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Ala Glu Val Lys Tyr 
        115                 120                 125             


His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ile Ser Gln Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Gln Leu Lys Ala 
                165                 170                 175     


Glu Asn Thr Asn Val Gln Ala Leu Phe Lys Asp Phe Val Glu Val Tyr 
            180                 185                 190         


Asp Lys Thr Val Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             


Leu Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala His Tyr Pro Ala Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Phe Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     


Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Asp Leu Glu Gly Leu Leu Gly Glu Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             


Leu Phe Ala Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Val Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Lys Lys Phe Glu 
                325                 330                 335     


Asp Phe Ile Lys Val Asn Ala Leu Asp Gln Tyr Asn Ala Ile Phe Lys 
            340                 345                 350         


Asp Lys Asn Lys Lys Gly Tyr Ala Gly Tyr Ile Glu Ser Gly Val Lys 
        355                 360                 365             


Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Leu Gln Ile Asn 
    370                 375                 380                 


Gly Ser Gly Asp Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         


Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 


Ala Glu Tyr Lys Ala Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 


Ile Leu Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     


Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Asn Glu Gln Gly Glu Ala Lys Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 


Asp Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     


Val Asn Leu Thr Gly Leu Asp Lys Glu Asn Lys Val Phe Asn Ser Ser 
            580                 585                 590         


Leu Gly Thr Tyr His Asp Leu Arg Lys Ile Leu Asn Lys Ser Phe Leu 
        595                 600                 605             


Asp Asn Lys Glu Asn Ala Gln Ile Ile Glu Asp Ile Ile Gln Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr 
625                 630                 635                 640 


Ser Asp Ile Phe Thr Lys Ala Gln Leu Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Asn Lys Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
        675                 680                 685             


Tyr Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser 
    690                 695                 700                 


Phe Lys Glu Glu Ile Ala Lys Ala Gln Ile Ile Gly Asp Val Asp Asp 
705                 710                 715                 720 


Ile Ala Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly His Asn Pro Ala Asn Ile Ile Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Asp Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Leu Leu 
    770                 775                 780                 


Gln Asp Ser Leu Lys Asn Leu Asp Asn Pro Val Asn Ile Lys Asn Val 
785                 790                 795                 800 


Glu Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln 
                805                 810                 815     


Asn Gly Lys Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asn Asn Leu 
            820                 825                 830         


Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Tyr Ile Lys Asp 
        835                 840                 845             


Asp Ser Phe Asp Asn Arg Val Leu Thr Ser Ser Ser Glu Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Cys Ala Arg Lys Ala 
865                 870                 875                 880 


Asp Trp Met Arg Leu Arg Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asn Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Val Leu Asp Ala Arg Phe Asn Ala Lys His Asp Glu 
    930                 935                 940                 


Asn Lys Lys Val Ile Arg Asp Val Lys Ile Ile Thr Leu Lys Ser Asn 
945                 950                 955                 960 


Leu Val Ser Gln Phe Arg Lys Asp Phe Lys Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Ile 
            980                 985                 990         


Gly Thr Ala Leu Leu Lys Lys Tyr  Pro Lys Leu Ala Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Glu Phe Lys Lys Tyr  Asp Val Arg Lys Phe  Ile Ala Lys 
    1010                 1015                 1020             


Ser Asp  Lys Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Leu Met Asn Phe Phe  Lys Lys Glu Val Lys  Phe Ala Asp 
    1040                 1045                 1050             


Gly Thr  Val Val Glu Arg Pro  Asp Ile Glu Thr Ser  Glu Asp Gly 
    1055                 1060                 1065             


Glu Ile  Ala Trp Asn Lys Gln  Thr Asp Phe Lys Ile  Val Arg Lys 
    1070                 1075                 1080             


Val Leu  Ser Tyr Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Val 
    1085                 1090                 1095             


Gln Thr  His Gly Leu Asp Arg  Gly Lys Pro Arg Gly  Leu Phe Asn 
    1100                 1105                 1110             


Ala Asn  Pro Ser Pro Lys Pro  Lys Pro Asp Ser Ser  Glu Asn Leu 
    1115                 1120                 1125             


Val Gly  Val Lys Arg Asn Leu  Asp Pro Lys Lys Tyr  Gly Gly Tyr 
    1130                 1135                 1140             


Ala Gly  Ile Ser Asn Ser Tyr  Ala Val Leu Val Lys  Ala Ile Ile 
    1145                 1150                 1155             


Glu Lys  Gly Val Lys Lys Lys  Glu Thr Met Val Leu  Glu Phe Gln 
    1160                 1165                 1170             


Gly Ile  Ser Ile Leu Asp Arg  Ile Thr Phe Glu Lys  Asp Lys Arg 
    1175                 1180                 1185             


Ala Phe  Leu Leu Gly Lys Gly  Tyr Lys Asp Ile Lys  Lys Ile Ile 
    1190                 1195                 1200             


Glu Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu Lys Asp  Gly Ser Arg 
    1205                 1210                 1215             


Arg Met  Leu Ala Ser Ile Leu  Ser Thr Asn Asn Lys  Arg Gly Glu 
    1220                 1225                 1230             


Ile His  Lys Gly Asn Glu Leu  Phe Val Pro Gln Lys  Phe Thr Thr 
    1235                 1240                 1245             


Leu Leu  Tyr His Ala Lys Arg  Ile Asn Asn Pro Ile  Asn Lys Asp 
    1250                 1255                 1260             


His Ile  Glu Tyr Val Lys Lys  His Arg Asp Asp Phe  Lys Glu Leu 
    1265                 1270                 1275             


Leu Asn  Tyr Val Leu Glu Phe  Asn Glu Lys Tyr Val  Gly Ala Thr 
    1280                 1285                 1290             


Lys Asn  Gly Glu Arg Leu Lys  Glu Ala Val Ala Asp  Phe Asp Ser 
    1295                 1300                 1305             


Lys Ser  Asn Glu Glu Ile Cys  Thr Ser Phe Leu Gly  Ala Val Asn 
    1310                 1315                 1320             


Ser Lys  Asn Ala Gly Leu Phe  Glu Leu Thr Ser Leu  Gly Ser Ala 
    1325                 1330                 1335             


Ser Asp  Phe Glu Phe Leu Gly  Val Lys Ile Pro Arg  Tyr Arg Asp 
    1340                 1345                 1350             


Tyr Thr  Pro Ser Ser Leu Leu  Lys Asp Ser Thr Leu  Ile His Gln 
    1355                 1360                 1365             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu 
    1370                 1375                 1380             


Gly Glu  Asp 
    1385     


<210>  1116
<211>  1386
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1116

Met Thr Lys Ile Thr Glu Asp Tyr Ile Val Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asn Ser Cys Gly Trp Val Ala Met Asp Ser Lys Asn Thr Ile Leu Arg 
            20                  25                  30          


Met His Gly Lys Thr Ala Ile Gly Ser His Leu Phe Asp Ala Gly Asn 
        35                  40                  45              


Ser Ala Ala Asp Arg Arg Ala Phe Arg Thr Thr Arg Arg Arg Ile Lys 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Lys Leu Leu Glu Glu Ile Phe Asp Ser His 
65                  70                  75                  80  


Leu Ala Lys Val Asp Pro Tyr Phe Phe Ala Arg Leu Lys Glu Ser Gly 
                85                  90                  95      


Leu Ser Pro Leu Asp Thr Arg Lys Asn Val Ser Ser Ile Ile Tyr Pro 
            100                 105                 110         


Thr Ser Leu Glu Asp Lys Lys Phe Tyr Val Asp Asn Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Tyr Lys Leu Met Thr Glu Asp Arg Lys Phe Asp Leu Arg 
    130                 135                 140                 


Glu Val Tyr Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn Phe 
145                 150                 155                 160 


Leu Tyr Asn Thr Pro Val Lys Ala Phe Glu Ala Ser Lys Ile Asp Val 
                165                 170                 175     


Lys Gly Ser Leu Lys Lys Leu Asn Glu Leu Tyr Glu Gly Leu Asp Ser 
            180                 185                 190         


Glu Phe Asn Val Glu Leu Asp Ser Asn Asn Ala Leu Glu Ile Glu Asn 
        195                 200                 205             


Ile Ile Arg Asp Lys Lys Ile Phe Lys Ile Asn Lys Val Lys Glu Ile 
    210                 215                 220                 


Asn Gln Leu Leu Ser Leu Lys Thr Glu Asp Lys Glu Arg Thr Lys Leu 
225                 230                 235                 240 


Met Lys Asp Val Ser Lys Gln Ile Val Asn Ala Ile Leu Gly Tyr Lys 
                245                 250                 255     


Thr Lys Phe Glu Thr Ile Leu Leu Lys Asp Val Ser Lys Asp Glu Ala 
            260                 265                 270         


Asp Asp Trp Glu Phe Lys Leu Thr Asp Val Asp Ala Asp Asn Lys Phe 
        275                 280                 285             


Glu Asn Leu Ile Gly Asp Leu Asn Glu Asn Glu Gln Glu Ile Leu Leu 
    290                 295                 300                 


Val Ile Arg Lys Leu Ala Asn Ala Ile Thr Leu Ser Asn Ile Val Glu 
305                 310                 315                 320 


Glu Gly Lys Thr Leu Ser Glu Ser Met Lys Asp Lys Tyr Lys Lys His 
                325                 330                 335     


Ser Asp Asp Leu Lys Leu Leu Lys Gln Val Ile Ser Asp His Pro Asp 
            340                 345                 350         


Arg Asp Lys Ala Lys Lys Leu Ala Leu Ala Tyr Asp Leu Tyr Val Asn 
        355                 360                 365             


Asn Arg His Gly Lys Ile Leu Gln Ala Lys Asp Ile Leu Glu Lys Lys 
    370                 375                 380                 


Lys Thr Leu Ser Lys Glu Asp Phe Tyr Lys Glu Ile Lys Lys Asn Leu 
385                 390                 395                 400 


Asp Asp Ser Lys Glu Ser Gln Glu Ile Leu Asp Ala Ile Ser Leu Asp 
                405                 410                 415     


Ser Phe Met Pro Lys Gln Arg Thr Asn Glu Asn Gly Val Ile Pro Tyr 
            420                 425                 430         


Gln Leu His Gln Leu Glu Leu Asp Lys Ile Ile Glu Asn Gln Gly Lys 
        435                 440                 445             


Tyr Tyr Pro Phe Leu Lys Glu Val Asn Pro Val Ser Ser His Leu Lys 
    450                 455                 460                 


Gln Ala Pro Tyr Lys Leu Asp Glu Leu Ile Arg Phe Arg Val Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ile Ser Pro Asn Glu Ser Thr Lys Asn Lys Gln 
                485                 490                 495     


Thr Lys Lys Asn Gln Asn Phe Ala Trp Met Ile Arg Lys Glu Gln Gly 
            500                 505                 510         


Gln Ile Thr Pro Trp Asn Phe Asp Gln Lys Val Asp Arg Met Ala Ser 
        515                 520                 525             


Ala Asn Lys Phe Ile Lys Arg Met Thr Thr Ile Asp Thr Tyr Leu Leu 
    530                 535                 540                 


Gly Glu Asp Val Leu Pro Ala Asn Ser Leu Leu Tyr Gln Lys Phe Thr 
545                 550                 555                 560 


Val Leu Asn Glu Leu Asn Asn Val Lys Ile Asn Gly Lys Arg Ile Ser 
                565                 570                 575     


Val Pro Leu Lys Gln Glu Leu Tyr Asn Asn Leu Phe Lys Lys Asn Ser 
            580                 585                 590         


Thr Val Thr Thr Asn Lys Leu Lys Ser Tyr Leu Lys Glu Asn Tyr Lys 
        595                 600                 605             


Leu Ile Lys Val Glu Ile Lys Gly Leu Ser Asp Glu Lys Lys Phe Asn 
    610                 615                 620                 


Ser Gly Leu Thr Thr Tyr Asn Lys Leu Lys Asn Leu Lys Ile Phe Asp 
625                 630                 635                 640 


Gln Gln Ile Asp Asp Pro Asn Tyr Asn Lys Asp Phe Glu Arg Ile Ile 
                645                 650                 655     


Glu Trp Ser Thr Ile Phe Glu Asp Lys Ala Ile Tyr Lys Glu Lys Leu 
            660                 665                 670         


Lys Thr Ile Asp Trp Leu Ser Asp Arg Gln Ile Asp Lys Leu Ser Lys 
        675                 680                 685             


Ile Arg Met Gln Gly Trp Gly Gln Leu Ser Lys Lys Leu Leu Ser Gln 
    690                 695                 700                 


Leu Thr Asp Asn Asn Gly Gln Thr Ile Ile Glu Gln Leu Trp Asp Ser 
705                 710                 715                 720 


Gln Asn Asn Phe Met Gln Ile Val Asn Gln Ala Asp Phe Lys Asp Ala 
                725                 730                 735     


Ile Ala Val Ala Asn Gln Asn Leu Leu Val Asp Thr Ser Val Glu Asp 
            740                 745                 750         


Ile Leu Asn Glu Ala Tyr Thr Ser Pro Ala Asn Lys Lys Ala Ile Arg 
        755                 760                 765             


Gln Val Val Lys Val Val Asp Asp Ile Val Lys Ala Thr Ser Gly Lys 
    770                 775                 780                 


Val Pro Lys Gln Ile Ala Ile Glu Phe Thr Arg Asp Ala Asp Asp Asn 
785                 790                 795                 800 


Ala Lys Ile Ser Gln Ser Arg Ala Asn Lys Leu Arg Lys Val Tyr Lys 
                805                 810                 815     


Glu Met Ser Asn Glu Leu Ala Ser Glu Thr Ile Arg Asn Glu Leu Glu 
            820                 825                 830         


Arg Val Ala Lys Asp Gln Lys Leu Leu Lys Asp Lys His Tyr Leu Tyr 
        835                 840                 845             


Phe Met Gln Leu Gly Arg Asp Ala Tyr Thr Gly Glu Leu Ile Asp Ile 
    850                 855                 860                 


Asp Asn Leu Glu Gln Tyr Asp Ile Asp His Val Leu Pro Gln Ser Phe 
865                 870                 875                 880 


Ile Lys Asp Asp Ser Leu Glu Asn Arg Val Leu Val Lys Lys Ala Val 
                885                 890                 895     


Asn Asn Gly Lys Ser Asp Asn Val Pro Val Lys Leu Tyr Gly Asn Arg 
            900                 905                 910         


Met Ala Thr Asp Leu Gly Met Thr Ile Arg His Met Trp Glu Lys Trp 
        915                 920                 925             


Lys Glu Gln Gly Leu Ile Thr Lys Thr Lys Tyr Asn Asn Leu Ile Ile 
    930                 935                 940                 


Asp Pro Asp Lys Ile Asn Lys Tyr Glu Ser Ser Gly Phe Ile His Arg 
945                 950                 955                 960 


Gln Leu Val Glu Thr Ser Gln Ile Ile Lys Leu Val Ser Thr Ile Leu 
                965                 970                 975     


Gln Ser Lys Tyr Pro Asp Thr Glu Ile Ile Val Val Lys Ala Arg Tyr 
            980                 985                 990         


Asn His Tyr Leu Arg Lys Arg Leu  Asn Leu Tyr Lys Ser  Arg Glu Val 
        995                 1000                 1005             


Asn Asp  Tyr His His Ala Ile  Asp Ala Tyr Leu Ser  Ala Ile Cys 
    1010                 1015                 1020             


Gly Asn  Leu Leu Tyr Gln Val  Tyr Pro Tyr Leu Arg  Pro Phe Phe 
    1025                 1030                 1035             


Val Tyr  Gly Gln Tyr Lys Lys  Phe Ser Ser Glu Pro  Glu Lys Glu 
    1040                 1045                 1050             


Lys Ile  Ile Tyr Asp Lys Thr  Arg Arg Tyr Asn Phe  Ile Ser Gln 
    1055                 1060                 1065             


Ile Phe  Glu Asn Lys Asp Asn  Asp Ile Ile Ser Arg  Glu Thr Lys 
    1070                 1075                 1080             


Lys Lys  Val Phe Asp Lys Lys  Asp Ile Ile Glu Lys  Leu Lys Arg 
    1085                 1090                 1095             


Ala Tyr  Asn Tyr Lys Tyr Met  Leu Val Ser Arg Glu  Thr Glu Thr 
    1100                 1105                 1110             


His Asp  Gln Glu Met Phe Lys  Met Thr Val Tyr Pro  Arg Leu Ser 
    1115                 1120                 1125             


Arg Asp  Thr Lys Lys Ser Arg  Asn Leu Ile Pro Lys  Lys Lys Asp 
    1130                 1135                 1140             


Met Pro  Thr Glu Ile Tyr Gly  Gly Tyr Thr Asn Asn  Ser Asp Ala 
    1145                 1150                 1155             


Tyr Met  Ile Ile Ala Arg Ile  Asn Lys Lys Lys Gly  Pro Glu Tyr 
    1160                 1165                 1170             


Arg Val  Phe Gly Val Pro Met  Arg Glu Leu Val Asn  Leu Arg Lys 
    1175                 1180                 1185             


Ala Glu  Lys Asn Gly His Tyr  Lys Ala Tyr Leu Lys  Gln Val Leu 
    1190                 1195                 1200             


Glu Pro  Glu Ile Met Tyr Ser  Lys Asn Gly Lys Lys  Asn Lys Thr 
    1205                 1210                 1215             


Ile Ser  Ser Phe Glu Ile Val  Lys Ala Lys Val Pro  Tyr Lys Gln 
    1220                 1225                 1230             


Val Ile  Leu Asp Gly Asp Lys  Lys Phe Met Leu Ala  Ser Ser Thr 
    1235                 1240                 1245             


Tyr Val  Gln Asn Ala Lys Gln  Leu Thr Leu Ser Gln  Tyr Ser Met 
    1250                 1255                 1260             


Gln Ala  Ile Thr Asp Asn Cys  Glu Asn Asp Thr Glu  Glu Glu Lys 
    1265                 1270                 1275             


Ala Leu  Val Glu Ala Tyr Asp  Glu Ile Leu Thr Asn  Ile Asp Lys 
    1280                 1285                 1290             


Tyr Leu  Pro Leu Phe Asp Ile  Asn Lys Phe Arg Glu  Lys Leu His 
    1295                 1300                 1305             


Ala Gly  Arg Glu Lys Phe Val  Asp Leu Pro Leu Ala  Glu Lys Lys 
    1310                 1315                 1320             


Glu Thr  Ile Leu Gln Val Leu  Asn Gly Leu His Asp  Asn Ala Val 
    1325                 1330                 1335             


Met Pro  Lys Ile Lys Ser Leu  Gly Leu Ser Thr Pro  Leu Gly Phe 
    1340                 1345                 1350             


Met Gln  Phe His Thr Gly Val  Ser Leu Ser Glu Asn  Ala Lys Leu 
    1355                 1360                 1365             


Ile Tyr  Gln Ser Pro Thr Gly  Leu Phe Glu Lys Arg  Leu Lys Ile 
    1370                 1375                 1380             


Ser Asp  Leu 
    1385     


<210>  1117
<211>  1387
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1117

Met Ser Asp Ser Lys Lys Phe Glu Gly Glu Asn Val Lys Ser Gly Asp 
1               5                   10                  15      


Lys Lys Thr Asn Lys Asn His Gly Lys Val Leu Lys Ser Ser Pro Lys 
            20                  25                  30          


Glu Ile Arg Lys Asn Thr Glu Tyr Phe Ile Gly Leu Asp Ile Gly Thr 
        35                  40                  45              


Ser Ser Val Gly Trp Ala Val Thr Asn Thr Asp Tyr Glu Ile Leu Lys 
    50                  55                  60                  


Phe Asn Gly Lys Arg Met Trp Gly Ser Arg Leu Phe Asn Glu Ala Ser 
65                  70                  75                  80  


Thr Ala Glu Glu Arg Arg Gly His Arg Val Asn Arg Arg Arg Ile Gln 
                85                  90                  95      


Arg Arg Arg Asp Arg Leu Asn Leu Leu Glu Glu Ile Phe Ser Glu Glu 
            100                 105                 110         


Met Ala Lys Val Asp Pro Glu Phe Phe Met Arg Leu Lys Glu Ser Lys 
        115                 120                 125             


Phe His Leu Glu Asp Lys Asp Glu Arg Ile Leu Gly Glu Ser Asn Ile 
    130                 135                 140                 


Ile Phe Lys Gly Ser Glu Asp Gly Asp Lys Lys Tyr Tyr Glu Lys Tyr 
145                 150                 155                 160 


Pro Thr Ile Tyr His Leu Arg His Lys Leu Met Gln Ser Cys Glu Asp 
                165                 170                 175     


Val Asp Ile Arg Glu Leu Tyr Met Ala Leu His His Ile Ile Lys Tyr 
            180                 185                 190         


Arg Gly His Phe Leu Phe Lys Gly Asp Phe Glu Glu Thr Gly Thr Ser 
        195                 200                 205             


Ile Glu Asn Ile Ile Ile Glu Leu Leu Glu Leu Ile Asn Asn Asn Leu 
    210                 215                 220                 


Gly Val Lys Lys Phe Glu Tyr Asn Glu Ser Leu Ile Asn Glu Leu Lys 
225                 230                 235                 240 


Asn Ile Val Ser Asn Thr Ser Asn Thr Ile Thr Asn Lys Lys Lys Leu 
                245                 250                 255     


Leu Gly Glu Phe Phe Asn Lys Lys Glu Tyr Lys Ala His Lys Ser Leu 
            260                 265                 270         


Phe Ile Leu Ser Ile Gly Ala Ser Val Lys Pro Val Asp Leu Phe Leu 
        275                 280                 285             


Glu Glu Glu Tyr Lys Asp Ile Asp Asp Ile Lys Asn Ile Ser Phe Lys 
    290                 295                 300                 


Asn Leu Glu Tyr Asp Gln Val Arg Asp Thr Tyr Glu Ser Ile Phe Leu 
305                 310                 315                 320 


Asp Lys Ile Glu Phe Ile Asp Val Ala Lys Arg Leu Tyr Asp Ala Thr 
                325                 330                 335     


Ile Leu Leu Ser Ile Lys Pro Glu Gly Lys Thr Leu Ser Glu Ala Lys 
            340                 345                 350         


Val Asp Ile Tyr Asn Lys His His Asn Asp Leu Glu Leu Leu Lys Lys 
        355                 360                 365             


Val Leu Lys Thr Asp Lys Glu Lys Tyr Asp Ser Ile Leu Lys Leu Asp 
    370                 375                 380                 


Ile Lys Lys Gly Asn Asn Tyr Val Asn Tyr Val Lys Lys Ser Lys Asn 
385                 390                 395                 400 


Gly Ile Ser Cys Ser Lys Glu Glu Phe His Asn Ala Leu Lys Lys Val 
                405                 410                 415     


Leu Val Lys Ile Glu Lys Thr Asp Asp Ile Lys Tyr Ile Leu Asn Glu 
            420                 425                 430         


Ile Glu Leu Glu Arg Phe Leu Pro Leu Gln Arg Val Lys Asp Asn Ser 
        435                 440                 445             


Val Ile Pro Tyr Gln Ile His Arg Tyr Glu Leu Lys Lys Ile Leu Glu 
    450                 455                 460                 


Asn Ala Ser Lys Lys Phe Thr Phe Leu Asn Glu Glu Ser Asp Gly His 
465                 470                 475                 480 


Thr Asn Ile Glu Lys Ile Ile Lys Ile Met Glu Tyr Arg Ile Pro Tyr 
                485                 490                 495     


Tyr Val Gly Pro Leu Asn Asp His His Ser Val Tyr Asn Asn Gly Phe 
            500                 505                 510         


Ala Trp Val Ser Arg Lys Arg Ser Gly Arg Ile Thr Pro Trp Asn Phe 
        515                 520                 525             


Asp Glu Met Val Asp Lys Glu Glu Ser Ala Glu Arg Phe Ile Glu Asn 
    530                 535                 540                 


Leu Thr Asn Lys Cys Ser Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys 
545                 550                 555                 560 


Glu Ser Leu Leu Tyr Ser Glu Phe Ser Leu Leu Asn Glu Leu Asn Asn 
                565                 570                 575     


Ile Lys Tyr Asp Gly Ile Ser Leu Pro Ile Asp Ile Lys Lys Ser Ile 
            580                 585                 590         


Val Glu Lys Ile Phe Arg Val Glu Lys Lys Arg Val Thr Lys Lys Tyr 
        595                 600                 605             


Ile Lys Glu Phe Leu Lys Gln Glu Gly Tyr Ser Asp Gly Lys Gly Glu 
    610                 615                 620                 


Val Ser Gly Ile Asp Ile Asp Ile Lys Ser Asp Leu Lys Ser Tyr Arg 
625                 630                 635                 640 


Asp Met Val Lys Ile Phe Gly Thr Glu Ile Asp Tyr Arg Met Ala Glu 
                645                 650                 655     


Asn Ile Ile Lys Trp Val Thr Leu Phe Gly Asp Ser Lys Asp Leu Leu 
            660                 665                 670         


Lys Thr Lys Ile Asn Lys Glu Tyr Gly Glu Lys Val Ser Lys Asp Gln 
        675                 680                 685             


Leu Lys Lys Val Cys Lys Leu Lys Tyr Arg Asp Trp Gly Arg Leu Ser 
    690                 695                 700                 


Lys Lys Phe Leu Thr Gly Ile Arg Ser Ser Val Leu Val Asn Asp Ala 
705                 710                 715                 720 


Thr Gly Glu Gly Tyr Thr Ile Ile Glu Ala Met Arg Asn Thr Asp Asn 
                725                 730                 735     


Asn Leu Met Gln Leu Leu Arg Asn Glu Tyr Asp Tyr Leu Lys Lys Ile 
            740                 745                 750         


Asp Gln Ile Asn Met Leu Ser Arg Lys Asp Ile Asp Glu Ile Ser His 
        755                 760                 765             


Asp Ile Leu Asp Asp Leu Tyr Cys Ser Pro Ala Val Lys Arg Ser Leu 
    770                 775                 780                 


Trp Gln Thr Ile Lys Ile Val Glu Glu Ile Lys Lys Ile Met Gly His 
785                 790                 795                 800 


Glu Pro Lys Lys Val Phe Val Glu Val Thr Arg Thr Asn Lys Ala Asp 
                805                 810                 815     


Lys Lys Thr Thr Ile Thr Arg Lys Asn Arg Leu Leu Glu Leu Tyr Ser 
            820                 825                 830         


Ala Ile Asn Ala Glu Ser Arg Asp Trp Val Asn Glu Leu Asn Ser Thr 
        835                 840                 845             


Glu Val Ser Lys Phe Asn Ser Lys Lys Leu Tyr Leu Tyr Tyr Thr Gln 
    850                 855                 860                 


Met Gly Lys Cys Met Tyr Thr Gly Lys Asn Ile Asp Ile Glu Ser Leu 
865                 870                 875                 880 


Phe Thr Asp Met Tyr Asp Ile Asp His Ile Phe Pro Arg Ser Lys Thr 
                885                 890                 895     


Lys Asp Asn Ser Phe Glu Asn Leu Val Leu Val Thr Arg Lys Ala Asn 
            900                 905                 910         


Ala Asp Lys Arg Asp Val Tyr Pro Ile Asp Pro Lys Ile Gln Thr Glu 
        915                 920                 925             


Arg Lys Ser Leu Trp Asp Met Leu His Asp Lys Lys Leu Ile Gly Asp 
    930                 935                 940                 


Lys Lys Tyr Asp Arg Leu Thr Arg Thr Thr Gly Phe Thr Asp Asp Glu 
945                 950                 955                 960 


Met Ser Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Ser Gln Ala Ser 
                965                 970                 975     


Lys Ala Ala Ala Glu Met Leu Lys Leu Ile Asn Asn Asp Ser Glu Ile 
            980                 985                 990         


Val Tyr Val Lys Ala Glu Asn Val  Ser Asp Phe Arg His  Glu Asn Glu 
        995                 1000                 1005             


Phe Ile  Lys Val Arg Glu Ile  Asn Asp His His His  Ala Lys Asp 
    1010                 1015                 1020             


Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val Tyr Asn  Glu Lys Phe 
    1025                 1030                 1035             


Thr Lys  Asn Pro Ala Asn Tyr  Ile Lys Lys Gly Gly  Gly Asn Asn 
    1040                 1045                 1050             


Tyr Ser  Leu Asn Cys Ile Phe  Asn Arg Asn Leu Asp  Arg Asn Gly 
    1055                 1060                 1065             


Lys Arg  Ile Trp Asp Glu Lys  Lys Ser Ile Asp Thr  Val Asn Arg 
    1070                 1075                 1080             


Met Met  Lys Ser Asn Asp Val  Arg Val Thr Phe Lys  Asn Arg Val 
    1085                 1090                 1095             


Asp Lys  Gly Pro Ile Leu Asn  Lys Val Thr Ile His  Lys Ala Ser 
    1100                 1105                 1110             


Glu Phe  Met Asp Asp Gly Lys  Lys Asn Lys Lys Gly  Phe Pro Ile 
    1115                 1120                 1125             


Lys Ser  Ser Glu Lys Asn Gly  Asn Asn Arg Leu Cys  Asn Leu Tyr 
    1130                 1135                 1140             


Lys Tyr  Gly Gly Tyr Lys Asp  Leu Asn Tyr Ser His  Tyr Thr Ile 
    1145                 1150                 1155             


Val Arg  Tyr Asn Val Tyr Glu  Lys Lys Gly Leu Asp  Ile Lys Tyr 
    1160                 1165                 1170             


Tyr Met  Ile Pro Ile Pro Tyr  Tyr Leu Cys Leu Cys  Asn Ile Asn 
    1175                 1180                 1185             


Gln His  Asp Tyr Val Tyr Asp  Tyr Ile Ser Asn Arg  Ser Lys Lys 
    1190                 1195                 1200             


Asp Val  Asn Asn Ile Glu Ile  Ile Val Asn Glu Leu  Lys Met Gly 
    1205                 1210                 1215             


Ser Leu  Ile Asn Ile Asp Gly  Tyr Asn Tyr Tyr Leu  Gly Gly Ser 
    1220                 1225                 1230             


Lys Pro  Lys Thr Phe Tyr Val  Asp Pro Ala Tyr Gln  Ile Ile Leu 
    1235                 1240                 1245             


Asp Lys  Glu Ile Glu Arg Cys  Ile Lys Glu Ile Val  Lys Ala Lys 
    1250                 1255                 1260             


Asn Asn  Glu Tyr Arg Phe Ile  Asn Thr Asp Lys Ile  Asn Lys Asn 
    1265                 1270                 1275             


Leu Asn  Met Lys Ile Tyr Lys  Tyr Ile Val Asn Lys  Lys Ile Ile 
    1280                 1285                 1290             


Gly Val  Phe Arg Asn Arg Lys  Trp Asp Lys Gly Glu  Tyr Leu Asn 
    1295                 1300                 1305             


Ser Ser  Glu Thr Ile Glu Lys  Phe Ser Gly Leu Ser  Val Glu Ser 
    1310                 1315                 1320             


Gln Cys  Asn Val Ile Leu Arg  Leu Ile Asn Asp Met  Arg Gly Ser 
    1325                 1330                 1335             


Asn Lys  Gly Gly Ala Asn Ser  Lys Phe Asp Glu Leu  Glu Met Thr 
    1340                 1345                 1350             


Ile Ser  Arg Ala Glu Ile Pro  Gln Thr Leu Ser Asn  Val Ser Ser 
    1355                 1360                 1365             


Phe Lys  Ile Ile Asn Gln Ser  Ile Thr Gly Leu Phe  Glu Asn Glu 
    1370                 1375                 1380             


Val Thr  Ile Val 
    1385         


<210>  1118
<211>  1388
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1118

Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         


Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             


His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 


Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         


Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             


Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu Glu Lys Lys Asp Arg 
    210                 215                 220                 


Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr Phe 
                245                 250                 255     


Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             


Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Asn Gly Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 


Met Ile Met Arg Tyr Lys Glu His Glu Glu Asp Leu Gly Leu Leu Lys 
                325                 330                 335     


Ala Tyr Ile Arg Asn Ile Ser Leu Glu Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         


Asp Asn Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             


Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu Leu Ser Asn Phe Glu 
    370                 375                 380                 


Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Ile Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Asn Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 


Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 


Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             


Phe Ile Ala Glu Ser Met Ser Lys Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 


Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 


Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Asp Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             


Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
625                 630                 635                 640 


Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         


Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 


Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Glu Asp Lys Gly Asn 
705                 710                 715                 720 


Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg 
    770                 775                 780                 


Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn 
785                 790                 795                 800 


Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp 
                805                 810                 815     


Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly 
            820                 825                 830         


Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile 
        835                 840                 845             


Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile Asp Asn Lys Val Leu 
    850                 855                 860                 


Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu 
865                 870                 875                 880 


Glu Val Val Lys Lys Arg Lys Thr Leu Trp Tyr Gln Leu Leu Lys Ser 
                885                 890                 895     


Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
            900                 905                 910         


Gly Gly Leu Ser Arg Glu Asp Lys Ala Ser Phe Ile Gln Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu 
    930                 935                 940                 


Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg Ala Val Arg Thr Val 
945                 950                 955                 960 


Lys Ile Ile Thr Leu Lys Ser Thr Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Phe Glu Leu Tyr Lys Val Arg Glu Ile Asn Asp Leu His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Ser Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Lys  Leu Glu Pro Glu Phe  Val Tyr Gly Asp Tyr  Pro Lys Tyr 
    1010                 1015                 1020             


Asn Ser  Phe Arg Glu Arg Lys  Ser Ala Thr Glu Lys  Val Tyr Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Ile  Phe Lys Lys Ser Ile  Ser Leu Ala 
    1040                 1045                 1050             


Asp Gly  Thr Val Ile Glu Lys  Pro Leu Ile Glu Val  Asn Glu Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ser Val Trp Asn  Lys Glu Ser Asp Leu  Ala Thr Val 
    1070                 1075                 1080             


Arg Arg  Val Leu Ser Tyr Pro  Gln Val Asn Val Val  Lys Lys Val 
    1085                 1090                 1095             


Glu Lys  Gln Asn His Gly Leu  Asp Arg Gly Lys Pro  Lys Gly Leu 
    1100                 1105                 1110             


Phe Asn  Ala Asn Leu Ser Ser  Lys Pro Lys Pro Asn  Ser Asn Glu 
    1115                 1120                 1125             


Asn Leu  Val Gly Ala Lys Glu  Tyr Leu Asp Pro Lys  Lys Tyr Gly 
    1130                 1135                 1140             


Gly Tyr  Ala Gly Ile Ser Asn  Ser Phe Ala Val Leu  Val Lys Gly 
    1145                 1150                 1155             


Thr Ile  Glu Lys Gly Ala Lys  Lys Lys Ile Thr Asn  Val Leu Glu 
    1160                 1165                 1170             


Phe Gln  Gly Ile Ser Ile Leu  Asp Arg Ile Asn Tyr  Arg Lys Asp 
    1175                 1180                 1185             


Lys Leu  Asn Phe Leu Leu Glu  Lys Gly Tyr Lys Asp  Ile Glu Leu 
    1190                 1195                 1200             


Ile Ile  Glu Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Ser Asp Gly 
    1205                 1210                 1215             


Ser Arg  Arg Met Leu Ala Ser  Ile Leu Ser Thr Asn  Asn Lys Arg 
    1220                 1225                 1230             


Gly Glu  Ile His Lys Gly Asn  Gln Ile Phe Leu Ser  Gln Lys Phe 
    1235                 1240                 1245             


Val Lys  Leu Leu Tyr His Ala  Lys Arg Ile Ser Asn  Thr Ile Asn 
    1250                 1255                 1260             


Glu Asn  His Arg Lys Tyr Val  Glu Asn His Lys Lys  Glu Phe Glu 
    1265                 1270                 1275             


Glu Leu  Phe Tyr Tyr Ile Leu  Glu Phe Asn Glu Asn  Tyr Val Gly 
    1280                 1285                 1290             


Ala Lys  Lys Asn Gly Lys Leu  Leu Asn Ser Ala Phe  Gln Ser Trp 
    1295                 1300                 1305             


Gln Asn  His Ser Ile Asp Glu  Leu Cys Ser Ser Phe  Ile Gly Pro 
    1310                 1315                 1320             


Thr Gly  Ser Glu Arg Lys Gly  Leu Phe Glu Leu Thr  Ser Arg Gly 
    1325                 1330                 1335             


Ser Ala  Ala Asp Phe Glu Phe  Leu Gly Val Lys Ile  Pro Arg Tyr 
    1340                 1345                 1350             


Arg Asp  Tyr Thr Pro Ser Ser  Leu Leu Lys Asp Ala  Thr Leu Ile 
    1355                 1360                 1365             


His Gln  Ser Val Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ala 
    1370                 1375                 1380             


Lys Leu  Gly Glu Gly 
    1385             


<210>  1119
<211>  1388
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1119

Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asn Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Val Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         


Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Val Tyr 
        115                 120                 125             


His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 


Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         


Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             


Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu Glu Lys Lys Asp Arg 
    210                 215                 220                 


Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Arg Lys Cys Phe 
                245                 250                 255     


Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             


Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Phe Leu Thr Val Thr Asp Asn Glu Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asn Glu His Lys Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     


Glu Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         


Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             


Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu Leu Ala Glu Phe Glu 
    370                 375                 380                 


Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 


Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 


Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             


Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 


Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 


Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Tyr Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             


Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
625                 630                 635                 640 


Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         


Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 


Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Glu Asp Lys Gly Asn 
705                 710                 715                 720 


Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg 
    770                 775                 780                 


Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn 
785                 790                 795                 800 


Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp 
                805                 810                 815     


Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly 
            820                 825                 830         


Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile 
        835                 840                 845             


Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile Asp Asn Lys Val Leu 
    850                 855                 860                 


Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp Asp Phe Pro Ser Leu 
865                 870                 875                 880 


Glu Val Val Lys Lys Arg Lys Thr Phe Trp Tyr Gln Leu Leu Lys Ser 
                885                 890                 895     


Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
            900                 905                 910         


Gly Gly Leu Leu Pro Glu Asp Lys Ala Gly Phe Ile Gln Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu 
    930                 935                 940                 


Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg Ala Val Arg Thr Val 
945                 950                 955                 960 


Lys Ile Ile Thr Leu Lys Ser Thr Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Phe Glu Leu Tyr Lys Val Arg Glu Ile Asn Asp Phe His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Ile  Ala Ser Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Lys  Leu Glu Pro Glu Phe  Val Tyr Gly Asp Tyr  Pro Lys Tyr 
    1010                 1015                 1020             


Asn Ser  Phe Arg Glu Arg Lys  Ser Ala Thr Glu Lys  Val Tyr Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Ile  Phe Lys Lys Ser Ile  Ser Leu Ala 
    1040                 1045                 1050             


Asp Gly  Arg Val Ile Glu Arg  Pro Leu Ile Glu Val  Asn Glu Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ser Val Trp Asn  Lys Glu Ser Asp Leu  Ala Thr Val 
    1070                 1075                 1080             


Arg Arg  Val Leu Ser Tyr Pro  Gln Val Asn Val Val  Lys Lys Val 
    1085                 1090                 1095             


Glu Glu  Gln Asn His Gly Leu  Asp Arg Gly Lys Pro  Lys Gly Leu 
    1100                 1105                 1110             


Phe Asn  Ala Asn Leu Ser Ser  Lys Pro Lys Pro Asn  Ser Asn Glu 
    1115                 1120                 1125             


Asn Leu  Val Gly Ala Lys Glu  Tyr Leu Asp Pro Lys  Lys Tyr Gly 
    1130                 1135                 1140             


Gly Tyr  Ala Gly Ile Ser Asn  Ser Phe Ala Val Leu  Val Lys Gly 
    1145                 1150                 1155             


Thr Ile  Glu Lys Gly Ala Lys  Lys Lys Ile Thr Asn  Val Leu Glu 
    1160                 1165                 1170             


Phe Gln  Gly Ile Ser Ile Leu  Asp Arg Ile Asn Tyr  Arg Lys Asp 
    1175                 1180                 1185             


Lys Leu  Asn Phe Leu Leu Glu  Lys Gly Tyr Lys Asp  Ile Glu Leu 
    1190                 1195                 1200             


Ile Ile  Glu Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Ser Asp Gly 
    1205                 1210                 1215             


Ser Arg  Arg Met Leu Ala Ser  Ile Leu Ser Thr Asn  Asn Lys Arg 
    1220                 1225                 1230             


Gly Glu  Ile His Lys Gly Asn  Gln Ile Phe Leu Ser  Gln Lys Phe 
    1235                 1240                 1245             


Val Lys  Leu Leu Tyr His Ala  Lys Arg Ile Ser Asn  Thr Ile Asn 
    1250                 1255                 1260             


Glu Asn  His Arg Lys Tyr Val  Glu Asn His Lys Lys  Glu Phe Glu 
    1265                 1270                 1275             


Glu Leu  Phe Tyr Tyr Ile Leu  Glu Phe Asn Glu Asn  Tyr Val Gly 
    1280                 1285                 1290             


Ala Lys  Lys Asn Gly Lys Leu  Leu Asn Ser Ala Phe  Gln Ser Trp 
    1295                 1300                 1305             


Gln Asn  His Ser Ile Asp Glu  Leu Cys Ser Ser Phe  Ile Gly Pro 
    1310                 1315                 1320             


Thr Gly  Ser Glu Arg Lys Gly  Leu Phe Glu Leu Thr  Ser Arg Gly 
    1325                 1330                 1335             


Ser Ala  Ala Asp Phe Glu Phe  Leu Gly Val Lys Ile  Pro Arg Tyr 
    1340                 1345                 1350             


Arg Asp  Tyr Thr Pro Ser Ser  Leu Leu Lys Asp Ala  Thr Leu Ile 
    1355                 1360                 1365             


His Gln  Ser Val Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ala 
    1370                 1375                 1380             


Lys Leu  Gly Glu Gly 
    1385             


<210>  1120
<211>  1388
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1120

Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asn Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Val Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         


Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             


His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 


Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         


Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             


Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu Glu Lys Lys Asp Arg 
    210                 215                 220                 


Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Arg Lys Cys Phe 
                245                 250                 255     


Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             


Val Phe Ile Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Phe Leu Thr Val Thr Asp Asn Glu Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asn Glu His Lys Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     


Glu Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         


Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             


Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu Leu Ala Glu Phe Glu 
    370                 375                 380                 


Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 


Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 


Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             


Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 


Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 


Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Tyr Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             


Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
625                 630                 635                 640 


Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         


Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 


Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Glu Asp Lys Gly Asn 
705                 710                 715                 720 


Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg 
    770                 775                 780                 


Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn 
785                 790                 795                 800 


Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp 
                805                 810                 815     


Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly 
            820                 825                 830         


Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile 
        835                 840                 845             


Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile Asp Asn Lys Val Leu 
    850                 855                 860                 


Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp Asp Phe Pro Ser Leu 
865                 870                 875                 880 


Glu Val Val Lys Lys Arg Lys Thr Phe Trp Tyr Gln Leu Leu Lys Ser 
                885                 890                 895     


Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
            900                 905                 910         


Gly Gly Leu Leu Pro Glu Asp Lys Ala Gly Phe Ile Gln Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu 
    930                 935                 940                 


Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg Ala Val Arg Thr Val 
945                 950                 955                 960 


Lys Ile Ile Thr Leu Lys Ser Thr Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Phe Glu Leu Tyr Lys Val Arg Glu Ile Asn Asp Phe His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Ile  Ala Ser Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Lys  Leu Glu Pro Glu Phe  Val Tyr Gly Asp Tyr  Pro Lys Tyr 
    1010                 1015                 1020             


Asn Ser  Phe Arg Glu Arg Lys  Ser Ala Thr Glu Lys  Val Tyr Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Ile  Phe Lys Lys Ser Ile  Ser Leu Ala 
    1040                 1045                 1050             


Asp Gly  Arg Val Ile Glu Arg  Pro Leu Ile Glu Val  Asn Glu Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ser Val Trp Asn  Lys Glu Ser Asp Leu  Ala Thr Val 
    1070                 1075                 1080             


Arg Arg  Val Leu Ser Tyr Pro  Gln Val Asn Val Val  Lys Lys Val 
    1085                 1090                 1095             


Glu Glu  Gln Asn His Gly Leu  Asp Arg Gly Lys Pro  Lys Gly Leu 
    1100                 1105                 1110             


Phe Asn  Ala Asn Leu Ser Ser  Lys Pro Lys Pro Asn  Ser Asn Glu 
    1115                 1120                 1125             


Asn Leu  Val Gly Ala Lys Glu  Tyr Leu Asp Pro Lys  Lys Tyr Gly 
    1130                 1135                 1140             


Gly Tyr  Ala Gly Ile Ser Asn  Ser Phe Ala Val Leu  Val Lys Gly 
    1145                 1150                 1155             


Thr Ile  Glu Lys Gly Ala Lys  Lys Lys Ile Thr Asn  Val Leu Glu 
    1160                 1165                 1170             


Phe Gln  Gly Ile Ser Ile Leu  Asp Arg Ile Asn Tyr  Arg Lys Asp 
    1175                 1180                 1185             


Lys Leu  Asn Phe Leu Leu Glu  Lys Gly Tyr Lys Asp  Ile Glu Leu 
    1190                 1195                 1200             


Ile Ile  Glu Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Ser Asp Gly 
    1205                 1210                 1215             


Ser Arg  Arg Met Leu Ala Ser  Ile Leu Ser Thr Asn  Asn Lys Arg 
    1220                 1225                 1230             


Gly Glu  Ile His Lys Gly Asn  Gln Ile Phe Leu Ser  Gln Lys Phe 
    1235                 1240                 1245             


Val Lys  Leu Leu Tyr His Ala  Lys Arg Ile Ser Asn  Thr Ile Asn 
    1250                 1255                 1260             


Glu Asn  His Arg Lys Tyr Val  Glu Asn His Lys Lys  Glu Phe Glu 
    1265                 1270                 1275             


Glu Leu  Phe Tyr Tyr Ile Leu  Glu Phe Asn Glu Asn  Tyr Val Gly 
    1280                 1285                 1290             


Ala Lys  Lys Asn Gly Lys Leu  Leu Asn Ser Ala Phe  Gln Ser Trp 
    1295                 1300                 1305             


Gln Asn  His Ser Ile Asp Glu  Leu Cys Ser Ser Phe  Ile Gly Pro 
    1310                 1315                 1320             


Thr Gly  Ser Glu Arg Lys Gly  Leu Phe Glu Leu Thr  Ser Arg Gly 
    1325                 1330                 1335             


Ser Ala  Ala Asp Phe Glu Phe  Leu Gly Val Lys Ile  Gln Arg Tyr 
    1340                 1345                 1350             


Arg Asp  Tyr Thr Pro Ser Ser  Leu Leu Lys Asp Ala  Thr Leu Ile 
    1355                 1360                 1365             


His Gln  Ser Val Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ala 
    1370                 1375                 1380             


Lys Leu  Gly Glu Gly 
    1385             


<210>  1121
<211>  1388
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1121

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Glu Lys Glu Ile Ser Lys Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Ile Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Arg Pro Leu Ser Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Asn 
65                  70                  75                  80  


Leu Ile Glu Ile Leu Ile Asp Asn His Ile Ile Thr Lys Glu Thr Pro 
                85                  90                  95      


Leu Thr Glu Ile Gly Lys Gly Thr Thr Tyr Gln Thr Leu Ser Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Arg Glu Arg Ile Asp Leu Glu Asp Phe Ala Lys Val 
        115                 120                 125             


Leu Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Ser Asn Asp Glu Glu Gly Gln Ala Ile Asp Gly Met Ala Ile Ala Lys 
145                 150                 155                 160 


Glu Leu Tyr Glu Arg Gly Ile Thr Pro Gly Gln Tyr Val Leu Glu Leu 
                165                 170                 175     


Leu Glu Asn Asp Arg Lys Tyr Ile Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Lys Gln Glu Phe Asn Lys Ile Trp Asn Asn Gln Lys Thr Phe Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Glu Leu Phe Lys Glu Leu Asp Gly Lys Asn Lys 
    210                 215                 220                 


Ser Gln Thr Trp Ala Ile Cys Gln Lys Pro Phe Asn Ile Lys Gly Ile 
225                 230                 235                 240 


Lys Arg Ile Thr Lys Gly Arg Glu Lys Thr Ile Glu Asn Tyr His Trp 
                245                 250                 255     


Arg Val Lys Gly Leu Ser Gly Lys Leu Asn Leu Glu Lys Leu Ala Ile 
            260                 265                 270         


Ile Leu Gln Glu Ile Asn Asn Asp Ile Asn Gln Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Gly Ile Ser Asp Lys Ser Lys Lys Leu Tyr Leu Asn Lys Glu Thr 
    290                 295                 300                 


Val Gly Glu Phe Leu Tyr Lys Gln Ile Ala Lys Asn Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg His Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Lys Thr Gln Ser Asn Phe Asn Ala Asn Leu Thr Asp Glu 
            340                 345                 350         


Leu Lys Gln Glu Ile Arg Asp Val Ile Ile Phe Tyr Gln Arg Lys Ile 
        355                 360                 365             


Lys Ser Gln Lys Ser Leu Ile Ser Phe Cys Gln Phe Glu Ser Trp Glu 
    370                 375                 380                 


Gln Glu Tyr Phe Asp Lys Glu Thr Gly Lys Thr Lys Thr Arg Thr Val 
385                 390                 395                 400 


Gly Arg Arg Val Ile Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys 
                405                 410                 415     


Ile Trp Gln Asn Leu Asn His Leu Val Phe Thr Asn Ser Glu Thr Asp 
            420                 425                 430         


Glu Lys Ile Glu Ile Lys Glu Val Glu Gln Glu Ile Arg Lys Glu Leu 
        435                 440                 445             


Phe Glu Glu Leu Asn Leu Arg Gly Asp Leu Asn Pro Asn Asp Leu Leu 
    450                 455                 460                 


Lys Ile Leu Arg Ser Gln Phe Lys Ile Gly Lys Leu Ser Asn Trp Lys 
465                 470                 475                 480 


Cys Asn Phe Glu Lys Ile Glu Gly Asn Arg Thr Asn Ala Ala Leu Phe 
                485                 490                 495     


Asn Ile Tyr Gln Lys Ile Ser Asp Phe Glu Gly Tyr Gly Ile Asp Trp 
            500                 505                 510         


Asn Lys Ser Asn Ala Lys Lys Ile Arg Lys Glu Leu Lys Ala Thr Phe 
        515                 520                 525             


Pro Glu Ile Gly Ile Asp Ala Ser Ile Leu Glu Phe Asp Ala Tyr Lys 
    530                 535                 540                 


Lys Gln Leu Thr Asn Gln Ser Ser Tyr Glu Leu Trp His Leu Leu Tyr 
545                 550                 555                 560 


Ser Ala Glu Glu Asp Phe Lys Ile Ser Glu Gln Asp Lys Leu Ile Tyr 
                565                 570                 575     


Gly Asn Ser Ser Val Ser Leu Lys Lys Lys Leu His Leu Lys Phe Gly 
            580                 585                 590         


Phe Asn Pro Glu Tyr Ala Lys Met Leu Ala Asn Leu Ser Leu Met Ser 
        595                 600                 605             


Asp Tyr Gly Asn Leu Ser Ala Lys Ala Ile Lys Lys Ile Leu Pro Tyr 
    610                 615                 620                 


Leu Glu Asp Gly His Leu Tyr Ser Glu Ala Cys Arg Leu Ala Gly Tyr 
625                 630                 635                 640 


Asn His Ser Lys Ser Leu Thr Ala Glu Glu Leu Glu Lys Phe Glu Pro 
                645                 650                 655     


Lys Ala Lys Leu Asp Leu Leu Lys Lys Asn Ser Leu Arg Asn Pro Val 
            660                 665                 670         


Val Glu Lys Ile Leu Asn Gln Met Val Asn Val Val Asn Gln Val Ile 
        675                 680                 685             


Asp Thr Tyr Gly Lys Pro Asp Gly Val Arg Ile Glu Leu Ala Arg Glu 
    690                 695                 700                 


Leu Lys Lys Ser Ala Lys Glu Arg Ala Glu Met Thr Lys Gly Ile Ala 
705                 710                 715                 720 


Asp Gly Thr Arg Arg Asn Glu Glu Ile Lys Lys Ile Ile Thr Gln Lys 
                725                 730                 735     


Phe Gly Ile Pro Asn Pro Thr Lys Ser Asp Leu Val Arg Tyr Arg Leu 
            740                 745                 750         


Trp Gln Glu Leu Glu Ser Arg Gly Tyr Lys Asp Ile Phe Thr Asp Lys 
        755                 760                 765             


Tyr Ile Ser Pro Asp Lys Ile Phe Ser Ser Glu Ile Asp Ile Glu His 
    770                 775                 780                 


Ile Ile Pro Lys Ala Leu Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr 
785                 790                 795                 800 


Leu Ser Phe Arg Asn Val Asn Leu Lys Lys Ala Asn Arg Thr Ala Leu 
                805                 810                 815     


Asp Phe Ile Thr Gln Asp Tyr Gln Ser Asn Val Glu Ala Tyr Lys Glu 
            820                 825                 830         


Arg Val Glu Thr Trp Asn Lys Asp Asn His Ile Ser Lys Gly Lys Arg 
        835                 840                 845             


Asn Lys Leu Leu Met Ser Leu Lys Asp Ile Pro Asp Gly Phe Ile Glu 
    850                 855                 860                 


Arg Asp Leu Arg Asn Ser Gln Tyr Ile Ala Arg Lys Ala Lys Arg Met 
865                 870                 875                 880 


Leu Tyr Glu Val Phe Pro Asn Glu Val Leu Ala Thr Ser Gly Ser Ile 
                885                 890                 895     


Thr Asp Lys Leu Arg Ser Asp Trp Asp Leu Ile Asn Val Met Lys Glu 
            900                 905                 910         


Leu Asn Leu Pro Lys Tyr Lys Ala Leu Gly Leu Thr Glu Tyr Glu Glu 
        915                 920                 925             


Arg Leu Asp Lys Gly Thr Glu Glu Leu Phe Lys His Glu Val Ile Lys 
    930                 935                 940                 


Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu 
945                 950                 955                 960 


Ala Val Ala Phe Thr Thr His Asn His Ile Gln Tyr Ile Asn Asn Leu 
                965                 970                 975     


Asn Ala Arg Lys Asp Ile Thr Gln Asp Asn His Pro Ile Ile Lys Asn 
            980                 985                 990         


Ile Glu Asn Leu Ile Thr Glu Val  Asn Ser Lys Gly Lys  Arg Val Phe 
        995                 1000                 1005             


Lys Ala  Pro Met Ser Ser Phe  Arg Glu Ser Ala Lys  Glu Gln Ile 
    1010                 1015                 1020             


Glu Ala  Ile Leu Val Ser Phe  Lys Asn Lys Asn Lys  Val Val Thr 
    1025                 1030                 1035             


Lys Asn  Ile Asn Gln Thr Lys  Lys Glu Gly Gly Phe  Asn Tyr Gln 
    1040                 1045                 1050             


Asn Ala  Lys Thr Pro Arg Gly  Gln Leu His Lys Glu  Thr Ile Tyr 
    1055                 1060                 1065             


Gly Lys  Ser Lys Gln Pro Leu  Glu Lys Pro Val Lys  Ile Asn Asn 
    1070                 1075                 1080             


Arg Phe  Cys Lys Lys Gln Val  Gly Leu Ile Ile Asn  Phe Lys Glu 
    1085                 1090                 1095             


Gln Glu  Ala Val Leu His His  Leu Lys Lys Tyr Asp  Asn Asn Glu 
    1100                 1105                 1110             


Lys Ile  Ala Phe Ser Asn Lys  Thr Leu Lys Ser Asp  Pro Ile Gln 
    1115                 1120                 1125             


Val Asn  Gly Ser Pro Leu Arg  Glu Val Lys Ile Phe  Glu Glu Ile 
    1130                 1135                 1140             


Phe Thr  Ile Arg Lys Asp Ile  Asn Pro Asp Asn Phe  Lys Thr Gln 
    1145                 1150                 1155             


Lys His  Leu Asp Lys Ile Ile  Asp Lys Gly Ile Lys  Glu Val Met 
    1160                 1165                 1170             


Lys Lys  Arg Leu Glu Glu Tyr  Asn Gly Asp Phe Lys  Thr Ala Phe 
    1175                 1180                 1185             


Ser Asp  Leu Asp Lys Asn Pro  Ile Trp Leu Asn Gln  Ser Lys Gly 
    1190                 1195                 1200             


Ile Thr  Ile Lys Arg Ala Thr  Ile Ser Gly Val Thr  Asn Ala Glu 
    1205                 1210                 1215             


Pro Leu  His Gln Ala Lys Asp  His Leu Gly Asn Ile  Ile Leu Asp 
    1220                 1225                 1230             


Asp Asn  Gly Lys Glu Ile Pro  Ile Asp Tyr Val Ser  Thr Gly Asn 
    1235                 1240                 1245             


Asn His  His Val Ala Ile Tyr  Gln Asp Glu Ser Gly  Asn Leu Gln 
    1250                 1255                 1260             


Glu Glu  Val Val Ser Phe Tyr  Glu Ala Val Ile Arg  Lys Asn Gln 
    1265                 1270                 1275             


Asp Gln  Pro Ile Ile Lys Lys  Arg His Pro Lys Gly  Trp Lys Phe 
    1280                 1285                 1290             


Leu Phe  Thr Met Lys Gln Asn  Glu Met Phe Val Phe  Ser Ser Glu 
    1295                 1300                 1305             


Asn Phe  Asn Pro Ser Glu Ile  Asp Leu Leu Asp Glu  Lys Asn Ala 
    1310                 1315                 1320             


Ala Ile  Ile Ser Pro Asn Leu  Phe Arg Val Gln Lys  Ile Ala Thr 
    1325                 1330                 1335             


Lys Asn  Tyr Met Phe Arg His  His Leu Glu Thr Glu  Val Thr Asn 
    1340                 1345                 1350             


Asn Leu  Asp Phe Thr Tyr Arg  His Phe Gln Ala Thr  Glu Trp Leu 
    1355                 1360                 1365             


Arg Asn  Leu Cys Lys Val Arg  Ile Asp His Ile Gly  Arg Ile Val 
    1370                 1375                 1380             


Gln Ile  Gly Glu Tyr 
    1385             


<210>  1122
<211>  1389
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1122

Met Ile Ile Arg Thr Tyr Glu Leu Lys Thr Ile Thr Thr Ile Ala Ile 
1               5                   10                  15      


Leu Lys Met Phe Gln Leu Leu Thr Gly Gly Leu Ser Met Thr Lys Leu 
            20                  25                  30          


Gly Lys Pro Tyr Gly Ile Gly Val Asp Ile Gly Ser Asn Ser Ile Gly 
        35                  40                  45              


Phe Ala Ala Val Asp Glu Asn Ser His Leu Ile Arg Leu Lys Gly Lys 
    50                  55                  60                  


Thr Val Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys Ala Ala Ala Asp 
65                  70                  75                  80  


Arg Arg Ala Ser Arg Thr Thr Arg Arg Arg Leu Ser Arg Asn Arg Trp 
                85                  90                  95      


Arg Leu Ser Phe Leu Arg Asp Phe Phe Glu Ser His Ile Thr Pro Thr 
            100                 105                 110         


Gly Pro Asn Phe Phe Met Arg Gln Lys Tyr Ser Glu Ile Ser Pro Lys 
        115                 120                 125             


Asp Lys Ala Arg Tyr Lys Tyr Glu Lys Arg Leu Phe Asn Asp Arg Thr 
    130                 135                 140                 


Asp Ala Glu Phe Tyr Gln Gln Tyr Pro Thr Met Tyr His Leu Arg Asn 
145                 150                 155                 160 


Arg Leu Leu Thr Asp Pro Ser Lys Ala Asp Val Arg Glu Ile Tyr Phe 
                165                 170                 175     


Ala Ile His His Ile Leu Lys Ser Arg Gly His Phe Leu Thr Ser Gly 
            180                 185                 190         


Tyr Ala Lys Asp Phe Asn Thr Asn Lys Val Ala Leu Asn Glu Ile Phe 
        195                 200                 205             


Pro Ala Leu Gln Asp Ala Tyr Ala Gln Val Tyr Pro Asp Leu Asp Ile 
    210                 215                 220                 


Thr Phe Asp Glu Asn Lys Val Asn Glu Phe Lys Thr Val Leu Leu Asn 
225                 230                 235                 240 


Glu Lys Ala Thr Pro Ser Asp Thr Gln Arg Ala Leu Val Asn Leu Leu 
                245                 250                 255     


Leu Ala Glu Asp Gly Asp Lys Asp Ile Leu Lys Gln Gln Lys Gln Val 
            260                 265                 270         


Leu Thr Glu Phe Ala Lys Ala Val Val Gly Leu Lys Thr Lys Leu Asn 
        275                 280                 285             


Val Ala Leu Gly Thr Glu Val Asp Ser Ser Glu Ala Thr Val Trp Asn 
    290                 295                 300                 


Phe Ser Leu Gly Gln Leu Asp Asp Lys Trp Ala Gly Ile Glu Ser Ala 
305                 310                 315                 320 


Met Thr Asp Glu Gly Thr Glu Ile Leu Asp Gln Ile Arg Asp Leu Tyr 
                325                 330                 335     


Arg Ala Arg Leu Leu Asn Gly Ile Val Pro Ala Gly Lys Thr Leu Ser 
            340                 345                 350         


Gln Ala Lys Val Asp Asp Tyr Ala Gln His His Glu Asp Leu Glu Leu 
        355                 360                 365             


Phe Lys Asp Tyr Leu Lys Gln Leu Glu Asp Asp Gly Thr Ala Lys Ala 
    370                 375                 380                 


Ile Arg Gln Leu Tyr Asp Arg Tyr Ile Asp Gly Asp Asp Ala Lys Pro 
385                 390                 395                 400 


Phe Val Arg Glu Asp Phe Val Lys Ala Leu Thr Lys Glu Val Thr Ala 
                405                 410                 415     


His Pro Asn Ala Lys Ser Pro Glu Leu Leu Glu Arg Leu Ala Gln Pro 
            420                 425                 430         


Asp Phe Met Leu Lys Gln Arg Asn Lys Ala Asn Gly Ala Ile Pro Val 
        435                 440                 445             


Gln Met Gln Gln Arg Glu Leu Asp Gln Ile Ile Lys Asn Gln Ser Val 
    450                 455                 460                 


Tyr Tyr Asp Trp Leu Ala Ala Pro Asn Pro Val Glu Lys His Arg Lys 
465                 470                 475                 480 


Ser Met Pro Tyr Gln Leu Asp Glu Leu Leu Asn Phe Arg Ile Pro Tyr 
                485                 490                 495     


Tyr Val Gly Pro Leu Val Thr Ala Lys Glu Gln Lys Ala Ala Gln Gly 
            500                 505                 510         


Gly Val Phe Ala Trp Met Val Arg Lys Asp Pro Asp Gly Asn Ile Thr 
        515                 520                 525             


Pro Tyr Asn Phe Asp Glu Lys Val Asp Arg Glu Ala Ser Ala Asn Thr 
    530                 535                 540                 


Phe Ile Gln Arg Met Lys Thr Thr Asp Thr Tyr Leu Ile Gly Glu Asp 
545                 550                 555                 560 


Val Leu Pro Lys Gln Ser Leu Leu Tyr Gln Arg Tyr Glu Val Leu Asn 
                565                 570                 575     


Glu Leu Asn Asn Val Arg Val Asn Asn Glu Lys Leu Ser Ile Glu Gln 
            580                 585                 590         


Lys Gln Gln Val Ile Arg Glu Leu Phe Glu Arg His Asn Ser Val Thr 
        595                 600                 605             


Ile Lys Gln Phe Ala Glu Asn Leu Arg Ala His Gly Asp Tyr Ala His 
    610                 615                 620                 


Ile Pro Glu Ile Arg Gly Leu Ala Asp Glu Lys Arg Phe Leu Ser Ser 
625                 630                 635                 640 


Leu Ser Thr Tyr Arg Gln Leu Lys Ser Leu Leu Pro Asp Ala Ile Asp 
                645                 650                 655     


Asp Pro Ala Lys Gln Ala Asp Ile Glu Asn Ile Ile Ala Trp Ser Thr 
            660                 665                 670         


Val Phe Glu Asp Ala Ala Ile Phe Lys Thr Lys Leu Lys Glu Ile Asn 
        675                 680                 685             


Trp Leu Asp Ser Gln Thr Ile Thr Lys Leu Ser Asn Ile Arg Tyr Arg 
    690                 695                 700                 


Gly Trp Gly Gln Phe Ser His Lys Phe Leu Asn Gly Leu Thr Leu Gly 
705                 710                 715                 720 


Asn Gly His Thr Ile Ile Gln Glu Leu Phe Leu Ser Asn Asn Asn Leu 
                725                 730                 735     


Met Gln Ile Leu Thr Asp Glu Thr Leu Gln Lys Lys Met Thr Glu Leu 
            740                 745                 750         


Asn Ala Asp Lys Leu Lys Ala Ala Asn Ile Asn Asp Ala Ile Asp Asp 
        755                 760                 765             


Ala Tyr Thr Ser Pro Ser Asn Lys Lys Ala Leu Arg Gln Val Leu Arg 
    770                 775                 780                 


Val Ile Glu Asp Ile Lys Arg Ala Ala Asp Gly Gln Asp Pro Ser Trp 
785                 790                 795                 800 


Leu Tyr Val Glu Thr Ala Asp Gly Gly Gly Thr Pro Gly Lys Arg Thr 
                805                 810                 815     


Arg Ala Arg Gln His Gln Leu Gln Glu Ile Tyr Ala Asn Ala Ala His 
            820                 825                 830         


Glu Leu Ile Asp Ser Ala Val Arg Gly Glu Leu Glu Asp Lys Ile Ala 
        835                 840                 845             


Asp Lys Ala Asp Phe Asn Asp Arg Leu Val Leu Tyr Phe Met Gln Gly 
    850                 855                 860                 


Gly Arg Asp Ile Tyr Thr Gly Thr Pro Leu Asn Ile Asp Gln Leu Ser 
865                 870                 875                 880 


Ser Tyr Asp Ile Asp His Ile Leu Pro Gln Ser Leu Ile Lys Asp Asn 
                885                 890                 895     


Ser Leu Asp Asn Arg Val Leu Val His Ala Ile Ile Asn Arg Glu Lys 
            900                 905                 910         


Asn Ala Thr Phe Ala Ser Thr Ile Tyr Ala Gln Lys Met Asn Ala Thr 
        915                 920                 925             


Trp Arg Gln Trp His Glu Ala Gly Leu Ile Ser Gly Arg Lys Leu Arg 
    930                 935                 940                 


Asn Leu Gln Met Arg Pro Asp Gln Ile Asp Lys Tyr Ala Ser Gly Phe 
945                 950                 955                 960 


Val Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys Leu Thr Glu 
                965                 970                 975     


Gln Ile Val Ala Ala Gln Tyr Pro Glu Thr Lys Ile Ile Ala Val Lys 
            980                 985                 990         


Ala Gly Leu Ser His Gln Leu Arg  Glu Glu Leu Glu Phe  Pro Lys Asn 
        995                 1000                 1005             


Arg Asp  Val Asn His Tyr His  His Ala Phe Asp Ala  Phe Leu Ala 
    1010                 1015                 1020             


Ala Arg  Ile Gly Thr Tyr Leu  Leu Lys Arg Phe Pro  Asn Leu Gln 
    1025                 1030                 1035             


Ala Phe  Phe Thr Tyr Gly Lys  Phe Lys Lys Ala Asp  Val Lys Lys 
    1040                 1045                 1050             


Leu Arg  Gly Phe Asn Phe Ile  Arg Asp Ile Thr His  Ala Glu Asp 
    1055                 1060                 1065             


Lys Ile  Val Val Lys Asp Thr  Gly Glu Val Ile Trp  Asp Lys Lys 
    1070                 1075                 1080             


Arg Asp  Val Asp Glu Leu Asp  Arg Ile Tyr Asn Phe  Lys Arg Met 
    1085                 1090                 1095             


Leu Ile  Thr His Glu Val His  Phe Glu Lys Ala Asp  Leu Phe Lys 
    1100                 1105                 1110             


Gln Thr  Val Tyr Gly Ala Lys  Asp Ser Lys Glu Ala  Gly Gly Lys 
    1115                 1120                 1125             


Lys Gln  Leu Ile Ser Lys Lys  Lys Gly Tyr Pro Val  Asp Ile Tyr 
    1130                 1135                 1140             


Gly Gly  Tyr Thr Gln Glu Thr  Gly Ser Tyr Leu Ser  Val Val Arg 
    1145                 1150                 1155             


Leu Thr  Lys Lys Ala Met Tyr  Ala Val Val Lys Val  Ser Thr Arg 
    1160                 1165                 1170             


Asp Ala  Ala Lys Leu Ala Val  Ala Lys Ser Ile Ser  Glu Gln Lys 
    1175                 1180                 1185             


Glu Asn  Glu Thr Leu Asn Asp  Ile Ile Asp Glu Lys  Leu Ser Lys 
    1190                 1195                 1200             


Ile Ser  Lys Asn Gly Lys Ile  Thr His Glu Leu Phe  Glu Val Val 
    1205                 1210                 1215             


Leu Pro  Arg Val Gly Gln Lys  Thr Leu Phe Lys Asn  Ser Lys Tyr 
    1220                 1225                 1230             


Asn Leu  Phe Leu Val Asn Ser  Asp Thr Tyr Met His  Asn Tyr Gln 
    1235                 1240                 1245             


Glu Leu  Trp Met Pro Arg Glu  Tyr Gln Lys Met Trp  Lys Asp Ile 
    1250                 1255                 1260             


Ser Leu  Ser Lys Tyr Gly Asp  Ala Gln Thr Glu Asp  Gln Leu Asp 
    1265                 1270                 1275             


Gln Ile  Phe Gly Phe Ile Val  Ser Gln Val Asn Ser  Tyr Phe Asn 
    1280                 1285                 1290             


Leu Tyr  Asp Ile Asn Gln Phe  Arg Lys Lys Leu Asn  Asp Ala Ala 
    1295                 1300                 1305             


Asp Lys  Phe Ala Thr Leu Pro  Met Arg Asp Thr Asp  Asp Met Gln 
    1310                 1315                 1320             


Gly Lys  Ile Ala Thr Ile Gly  Gln Leu Leu Ile Gly  Leu Gln Ala 
    1325                 1330                 1335             


Asn Ala  Ala Arg Ser Asp Leu  Arg Asn Leu Asp Ile  Lys Thr Pro 
    1340                 1345                 1350             


Leu Gly  Leu Leu Gln Ile Pro  Asn Gly Ile Ser Leu  Asp Ser Asp 
    1355                 1360                 1365             


Thr Ser  Val Val Tyr Gln Ser  Pro Thr Gly Leu Leu  Glu Arg Glu 
    1370                 1375                 1380             


Val His  Leu Ser Asp Leu 
    1385                 


<210>  1123
<211>  1389
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1123

Met Ala Arg Asp Tyr Ser Val Gly Leu Asp Ile Gly Thr Ser Ser Val 
1               5                   10                  15      


Gly Trp Ala Ala Ile Asp Asn Lys Tyr His Leu Ile Arg Ala Lys Ser 
            20                  25                  30          


Lys Asn Leu Ile Gly Val Arg Leu Phe Asp Ser Ala Val Thr Ala Glu 
        35                  40                  45              


Lys Arg Arg Gly Tyr Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg His 
    50                  55                  60                  


Trp Arg Leu Arg Leu Leu Asn Asp Ile Phe Ala Gly Pro Leu Thr Asp 
65                  70                  75                  80  


Phe Gly Asp Glu Asn Phe Leu Ala Arg Leu Lys Tyr Ser Trp Val His 
                85                  90                  95      


Pro Gln Asp Gln Ser Asn Gln Ala His Phe Ala Ala Gly Leu Leu Phe 
            100                 105                 110         


Asp Ser Lys Glu Gln Asp Lys Asp Phe Tyr Arg Lys Tyr Pro Thr Ile 
        115                 120                 125             


Tyr His Leu Arg Leu Ala Leu Met Asn Asp Asp Gln Lys His Asp Leu 
    130                 135                 140                 


Arg Glu Val Tyr Leu Ala Ile His His Leu Val Lys Tyr Arg Gly His 
145                 150                 155                 160 


Phe Leu Ile Glu Gly Asp Val Lys Ala Asp Ser Ala Phe Asp Val His 
                165                 170                 175     


Thr Phe Ala Asp Ala Ile Gln Arg Tyr Ala Glu Ser Asn Asn Ser Asp 
            180                 185                 190         


Glu Asn Leu Leu Gly Lys Ile Asp Glu Lys Lys Leu Ser Ala Ala Leu 
        195                 200                 205             


Thr Asp Lys His Gly Ser Lys Ser Gln Arg Ala Glu Thr Ala Glu Thr 
    210                 215                 220                 


Ala Phe Asp Ile Leu Asp Leu Gln Ser Lys Lys Gln Ile Gln Ala Ile 
225                 230                 235                 240 


Leu Lys Ser Val Val Gly Asn Gln Ala Asn Leu Met Ala Ile Phe Gly 
                245                 250                 255     


Leu Asp Ser Ser Ala Ile Ser Lys Asp Glu Gln Lys Asn Tyr Lys Phe 
            260                 265                 270         


Ser Phe Asp Asp Ala Asp Ile Asp Glu Lys Ile Ala Asp Ser Glu Ala 
        275                 280                 285             


Leu Leu Ser Asp Thr Glu Phe Glu Phe Leu Cys Asp Leu Lys Ala Ala 
    290                 295                 300                 


Phe Asp Gly Leu Thr Leu Lys Met Leu Leu Gly Asp Asp Lys Thr Val 
305                 310                 315                 320 


Ser Ala Ala Met Val Arg Arg Phe Asn Glu His Gln Lys Asp Trp Glu 
                325                 330                 335     


Tyr Ile Lys Ser His Ile Arg Asn Ala Lys Asn Ala Gly Asn Gly Leu 
            340                 345                 350         


Tyr Glu Lys Ser Lys Lys Phe Asp Gly Ile Asn Ala Ala Tyr Leu Ala 
        355                 360                 365             


Leu Gln Ser Asp Asn Glu Asp Asp Arg Lys Lys Ala Lys Lys Ile Phe 
    370                 375                 380                 


Gln Asp Glu Ile Ser Ser Ala Asp Ile Pro Asp Asp Val Lys Ala Asp 
385                 390                 395                 400 


Phe Leu Lys Lys Ile Asp Asp Asp Gln Phe Leu Pro Ile Gln Arg Thr 
                405                 410                 415     


Lys Asn Asn Gly Thr Ile Pro His Gln Leu His Arg Asn Glu Leu Glu 
            420                 425                 430         


Gln Ile Ile Glu Lys Gln Gly Ile Tyr Tyr Pro Phe Leu Lys Asp Thr 
        435                 440                 445             


Tyr Gln Glu Asn Ser His Glu Leu Asn Lys Ile Thr Ala Leu Ile Asn 
    450                 455                 460                 


Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Val Glu Glu Glu Gln Lys 
465                 470                 475                 480 


Ile Ala Asp Asp Gly Lys Asn Ile Pro Asp Pro Thr Asn His Trp Met 
                485                 490                 495     


Val Arg Lys Ser Asn Asp Thr Ile Thr Pro Trp Asn Leu Ser Gln Val 
            500                 505                 510         


Val Asp Leu Asp Lys Ser Gly Arg Arg Phe Ile Glu Arg Leu Thr Gly 
        515                 520                 525             


Thr Asp Thr Tyr Leu Ile Gly Glu Pro Thr Leu Pro Lys Asn Ser Leu 
    530                 535                 540                 


Leu Tyr Gln Lys Phe Asp Val Leu Gln Glu Leu Asn Asn Ile Arg Val 
545                 550                 555                 560 


Ser Gly Arg Arg Leu Asp Ile Arg Ala Lys Gln Asp Ala Phe Glu His 
                565                 570                 575     


Leu Phe Lys Val Gln Lys Thr Val Ser Ala Thr Asn Leu Lys Asp Phe 
            580                 585                 590         


Leu Val Gln Ala Gly Tyr Ile Ser Glu Asp Thr Gln Ile Glu Gly Leu 
        595                 600                 605             


Ala Asp Val Asn Gly Lys Asn Phe Asn Asn Ala Leu Thr Thr Tyr Asn 
    610                 615                 620                 


Tyr Leu Val Ser Val Leu Gly Arg Glu Phe Val Glu Asn Pro Ser Asn 
625                 630                 635                 640 


Glu Glu Leu Leu Glu Glu Ile Thr Glu Leu Gln Thr Val Phe Glu Asp 
                645                 650                 655     


Lys Lys Val Leu Arg Arg Gln Leu Asp Gln Leu Asp Gly Leu Ser Asp 
            660                 665                 670         


His Asn Arg Glu Lys Leu Ser Arg Lys His Tyr Thr Gly Trp Gly Arg 
        675                 680                 685             


Ile Ser Lys Lys Leu Leu Thr Thr Lys Ile Val Gln Asn Ala Asp Lys 
    690                 695                 700                 


Ile Asp Asn Gln Thr Phe Asp Val Pro Arg Met Asn Gln Ser Ile Ile 
705                 710                 715                 720 


Asp Thr Leu Tyr Asn Thr Lys Met Asn Leu Met Glu Ile Ile Asn Asn 
                725                 730                 735     


Ala Glu Asp Asp Phe Gly Val Arg Ala Trp Ile Asp Lys Gln Asn Thr 
            740                 745                 750         


Thr Asp Gly Asp Glu Gln Asp Val Tyr Ser Leu Ile Asp Glu Leu Ala 
        755                 760                 765             


Gly Pro Lys Glu Ile Lys Arg Gly Ile Val Gln Ser Phe Arg Ile Leu 
    770                 775                 780                 


Asp Asp Ile Thr Lys Ala Val Gly Tyr Ala Pro Lys Arg Val Tyr Leu 
785                 790                 795                 800 


Glu Phe Ala Arg Lys Thr Gln Glu Ser His Leu Thr Asn Ser Arg Lys 
                805                 810                 815     


Asn Gln Leu Ser Thr Leu Leu Lys Asn Ala Gly Leu Ser Glu Leu Val 
            820                 825                 830         


Thr Gln Val Ser Gln Tyr Asp Ala Ala Ala Leu Gln Asn Asp Arg Leu 
        835                 840                 845             


Tyr Leu Tyr Phe Leu Gln Gln Gly Lys Asp Met Tyr Ser Gly Glu Lys 
    850                 855                 860                 


Leu Asn Leu Asp Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro 
865                 870                 875                 880 


Gln Ala Tyr Thr Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val Ser 
                885                 890                 895     


Asn Ile Thr Asn Arg Arg Lys Ser Asp Ser Ser Asn Tyr Leu Pro Ala 
            900                 905                 910         


Leu Ile Asp Lys Met Arg Pro Phe Trp Ser Val Leu Ser Lys Gln Gly 
        915                 920                 925             


Leu Leu Ser Lys His Lys Phe Ala Asn Leu Thr Arg Thr Arg Asp Phe 
    930                 935                 940                 


Asp Asp Met Glu Lys Glu Arg Phe Ile Ala Arg Ser Leu Val Glu Thr 
945                 950                 955                 960 


Arg Gln Ile Ile Lys Asn Val Ala Ser Leu Ile Asp Ser His Phe Gly 
                965                 970                 975     


Gly Glu Thr Lys Ala Val Ala Ile Arg Ser Ser Leu Thr Ala Asp Met 
            980                 985                 990         


Arg Arg Tyr Val Asp Ile Pro Lys  Asn Arg Asp Ile Asn  Asp Tyr His 
        995                 1000                 1005             


His Ala  Phe Asp Ala Leu Leu  Phe Ser Thr Val Gly  Gln Tyr Thr 
    1010                 1015                 1020             


Glu Asn  Ser Gly Leu Met Lys  Lys Gly Gln Leu Ser  Asp Ser Ala 
    1025                 1030                 1035             


Gly Asn  Gln Tyr Asn Arg Tyr  Ile Lys Glu Trp Ile  His Ala Ala 
    1040                 1045                 1050             


Arg Leu  Asn Ala Gln Ser Gln  Arg Val Asn Pro Phe  Gly Phe Val 
    1055                 1060                 1065             


Val Gly  Ser Met Arg Asn Ala  Ala Pro Gly Lys Leu  Asn Pro Glu 
    1070                 1075                 1080             


Thr Gly  Glu Ile Thr Pro Glu  Glu Asn Ala Asp Trp  Ser Ile Ala 
    1085                 1090                 1095             


Asp Leu  Asp Tyr Leu His Lys  Val Met Asn Phe Arg  Lys Ile Thr 
    1100                 1105                 1110             


Val Thr  Arg Arg Leu Lys Asp  Gln Lys Gly Gln Leu  Tyr Asp Glu 
    1115                 1120                 1125             


Ser Arg  Tyr Pro Ser Val Leu  His Asp Ala Lys Ser  Lys Ala Ser 
    1130                 1135                 1140             


Ile Asn  Phe Asp Lys His Lys  Pro Val Asp Leu Tyr  Gly Gly Phe 
    1145                 1150                 1155             


Ser Ser  Ala Lys Pro Ala Tyr  Ala Ala Leu Ile Lys  Phe Lys Asn 
    1160                 1165                 1170             


Lys Phe  Arg Leu Val Asn Val  Leu Arg Gln Trp Thr  Tyr Ser Asp 
    1175                 1180                 1185             


Lys Asn  Ser Glu Asp Tyr Ile  Leu Glu Gln Ile Arg  Gly Lys Tyr 
    1190                 1195                 1200             


Pro Lys  Ala Glu Met Val Leu  Ser His Ile Pro Tyr  Gly Gln Leu 
    1205                 1210                 1215             


Val Lys  Lys Asp Gly Ala Leu  Val Thr Ile Ser Ser  Ala Thr Glu 
    1220                 1225                 1230             


Leu His  Asn Phe Glu Gln Leu  Trp Leu Pro Leu Ala  Asp Tyr Lys 
    1235                 1240                 1245             


Leu Ile  Asn Thr Leu Leu Lys  Thr Lys Glu Asp Asn  Leu Val Asp 
    1250                 1255                 1260             


Ile Leu  His Asn Arg Leu Asp  Leu Pro Glu Met Thr  Ile Glu Ser 
    1265                 1270                 1275             


Ala Phe  Tyr Lys Ala Phe Asp  Ser Ile Leu Ser Phe  Ala Phe Asn 
    1280                 1285                 1290             


Arg Tyr  Ala Leu His Gln Asn  Ala Leu Val Lys Leu  Gln Ala His 
    1295                 1300                 1305             


Arg Asp  Asp Phe Asn Ala Leu  Asn Tyr Glu Asp Lys  Gln Gln Thr 
    1310                 1315                 1320             


Leu Glu  Arg Ile Leu Asp Ala  Leu His Ala Ser Pro  Ala Ser Ser 
    1325                 1330                 1335             


Asp Leu  Lys Lys Ile Asn Leu  Ser Ser Gly Phe Gly  Arg Leu Phe 
    1340                 1345                 1350             


Ser Pro  Ser His Phe Thr Leu  Ala Asp Thr Asp Glu  Phe Ile Phe 
    1355                 1360                 1365             


Gln Ser  Val Thr Gly Leu Phe  Ser Thr Gln Lys Thr  Val Ala Gln 
    1370                 1375                 1380             


Leu Tyr  Gln Glu Thr Lys 
    1385                 


<210>  1124
<211>  1389
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1124

Met Lys Asn Tyr Asn Ile Ser Leu Val Pro Ser Pro Ser Gly Val Gly 
1               5                   10                  15      


Tyr Ala Val Thr Asp Asp Asn Leu Asn Ile Ile Lys Pro Val His His 
            20                  25                  30          


Val Ser Gly Ile Gly Ser Arg Leu Phe Asn Glu Gly Ser Ser Lys Gln 
        35                  40                  45              


Asp Arg Arg Asn Ser Arg Ser Ala Arg Arg Ser Arg His Arg Gln Gln 
    50                  55                  60                  


Gly Arg Ile Lys Ala Leu Asn Ser Ile Met Lys Asp Glu Ile Ile Lys 
65                  70                  75                  80  


Val Asp Pro Thr Phe Phe Asp Arg Leu Tyr Gln Ser Glu Leu Ser Pro 
                85                  90                  95      


Leu Asp Lys Asn Lys Lys Tyr Arg Asn Ile Ile Phe Asp Lys Pro Ser 
            100                 105                 110         


Val Glu Ser Tyr Tyr Tyr Lys Lys Phe Pro Thr Ile Tyr His Leu Glu 
        115                 120                 125             


Lys Tyr Leu Ile Asn Thr Asp Gly Lys Ala Asp Ile Arg Leu Ile Tyr 
    130                 135                 140                 


Trp Ala Leu His Ser Leu Leu Thr His Arg Gly His Phe Tyr Asn Thr 
145                 150                 155                 160 


Thr Pro Val Ser Gln Phe Lys Pro Gly Ala Leu Asn Ile Lys Asp Lys 
                165                 170                 175     


Phe Ile Glu Leu Asn Gln Leu Asn Gln Val Lys Thr Phe Glu Phe Ser 
            180                 185                 190         


Val Ala Asn Thr Pro Glu Ile Glu His Ile Leu Lys Asp Arg Tyr Ile 
        195                 200                 205             


Ser Lys Gln Asn Lys Ile Asn Gln Leu Ala Glu Leu Leu Phe Glu Gln 
    210                 215                 220                 


Glu Pro Glu Lys Asp Ile Gln Lys Ala Asn Thr Lys Val Ala Lys Thr 
225                 230                 235                 240 


Ile Ala Lys Ala Ile Leu Gly Asn Lys Phe Gln Leu Asp Glu Ile Ile 
                245                 250                 255     


Gly Leu Asp Val Asn Lys Ser Glu Lys Lys Glu Trp Ser Leu Gln Leu 
            260                 265                 270         


Ser Asp Leu Asn Leu Asp Ser Lys Ile Asp Glu Leu Ser Asp Ser Leu 
        275                 280                 285             


Thr Asp Gln Gln Leu Arg Ile Leu Glu Ile Leu Gln Glu Met Tyr Ala 
    290                 295                 300                 


Ala Thr Thr Leu Leu Asp Ile Leu Gln Gly Ser Asp Ser Leu Leu Asp 
305                 310                 315                 320 


Ala Lys Ile Lys Ser Tyr Asn Glu Tyr Lys Gln Asn Arg Leu Leu Phe 
                325                 330                 335     


Phe Asp Leu Leu Lys Thr Leu Pro Asp Lys Lys Ala His Leu Leu Arg 
            340                 345                 350         


Gln Gly Tyr Thr Leu Tyr Leu Asn Asn His His Arg Asp Leu Ala Ser 
        355                 360                 365             


Ala Arg Lys Asp Leu Gly Ile Val Ala Ala Thr Asn Phe Ser Lys Thr 
    370                 375                 380                 


Asp Leu Tyr Ala Leu Ile Lys Lys Thr Leu Lys Asp Glu Pro Glu Thr 
385                 390                 395                 400 


Pro Glu Ser Lys Lys Val Leu Gly Leu Ile Asp Lys Gly Glu Phe Leu 
                405                 410                 415     


Asn Lys Gln Arg Thr Asp Leu Asn Thr Tyr Ile Pro Tyr Gln Leu Asn 
            420                 425                 430         


Ala Leu Ile Leu Asn Lys Ile Leu Lys Asn Gln Gly Lys His Tyr Pro 
        435                 440                 445             


Phe Leu Ile Lys Glu Asn Pro Ala Glu Pro Asp Arg Lys Glu Ala Pro 
    450                 455                 460                 


Tyr Glu Ile Ser Gln Leu Met Gln Phe Thr Ile Pro Tyr Tyr Cys Gly 
465                 470                 475                 480 


Pro Leu Ala Thr Pro Glu Glu Gln Lys Asn Leu Pro Glu Ser Ser Arg 
                485                 490                 495     


Phe Gly Trp Leu Val Arg Lys Glu Glu Gly Arg Ile Thr Pro Trp Asn 
            500                 505                 510         


Phe Tyr Asn Lys Ile Asn Val Ile Glu Thr Ala Asn Ala Phe Ile Lys 
        515                 520                 525             


Arg Ser Ile Gly Lys Asp Thr Tyr Leu Leu Ser Glu Pro Val Leu Pro 
    530                 535                 540                 


Asp Asn Ser Leu Leu Tyr Gln Lys Tyr Lys Val Leu Asn Glu Leu Ser 
545                 550                 555                 560 


Ser Ile Ser Leu Val Asp Ala Asn Thr Lys Lys Glu Lys Lys Leu Thr 
                565                 570                 575     


Ala Lys Ile Lys Gln Leu Leu Tyr Lys Glu Gly Phe Lys Lys His Val 
            580                 585                 590         


Thr Val Ser Lys Thr Met Ala Leu Lys Ile Leu Asn Asp Asn Asn Val 
        595                 600                 605             


Pro Thr Ser Asp Ile Leu Gly Leu Ser Thr Gly Lys Lys Phe Asp Asn 
    610                 615                 620                 


Ser Leu Ser Thr Tyr Asn Ser Trp Lys Lys Ala Phe Pro Glu Gln Ile 
625                 630                 635                 640 


Asp Asn Pro His Tyr Arg Gln Asp Leu Glu Tyr Met Ile Glu Trp Ser 
                645                 650                 655     


Thr Val Phe Thr Asp Arg Lys Ile Leu Val Glu Lys Leu Lys Glu Ile 
            660                 665                 670         


Glu Trp Leu Arg Pro Glu Gln Ile Lys Phe Val Leu Glu His Arg Leu 
        675                 680                 685             


Thr Gly Trp Gly Lys Leu Ser Lys Arg Leu Leu Val Glu Leu Lys Asp 
    690                 695                 700                 


Asn Lys Gly Lys Ser Asn Ile Asp Asn Leu Phe Asn Thr Lys Lys Asn 
705                 710                 715                 720 


Phe Met Gln Ile Val Ser Gln Gln Val Tyr Asn Thr Gln Ile Glu Ala 
                725                 730                 735     


Ile Ala Leu Lys Thr Thr Lys Asn Gln Ser Leu Asp Asn Ile Leu Glu 
            740                 745                 750         


Ala Ala Tyr Thr Ser Pro Ala Asn Arg Lys Ala Ile Arg Gln Thr Met 
        755                 760                 765             


Lys Val Val Asn Glu Ile Val Thr Leu Ala Gly Lys Gly Thr His Pro 
    770                 775                 780                 


Asp Lys Ile Phe Leu Thr Phe Gln Arg Ser Lys Glu Gln Glu Gly Lys 
785                 790                 795                 800 


Leu Thr Thr Asp Arg Ala Ser Ser Leu Leu Lys Ile Tyr Arg Gly Ile 
                805                 810                 815     


Lys Asp Pro Leu Cys Thr Lys Glu Leu Val Asp Glu Leu Ser Lys Asn 
            820                 825                 830         


Val Arg Ala Asn Leu Ile Lys Thr Lys Glu Tyr Leu Tyr Tyr Gln Gln 
        835                 840                 845             


Leu Gly Arg Asp Ala Leu Thr Gly Glu Ile Ile Asp Lys Thr Asp Leu 
    850                 855                 860                 


Arg Asn Tyr Ala Val Leu His Ile Ile Pro Arg Ser Val Val Val Asp 
865                 870                 875                 880 


Asp Ser Ile Asn Asn Leu Val Leu Thr Lys Val Lys Asn Ala Lys Glu 
                885                 890                 895     


Ser Ile Val Val Gln Tyr Gly Ser Lys Asn Ile Ser Asp Ile Ser Met 
            900                 905                 910         


Ser Val Lys Asp Phe Trp Asn Arg Leu Gln Lys Leu Gly Leu Phe Ser 
        915                 920                 925             


Lys Gly Lys Leu Arg Asn Leu Thr Thr Asp Leu Thr Glu Leu Asn Gln 
    930                 935                 940                 


Tyr Glu Lys Gln Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Asn Gln 
945                 950                 955                 960 


Ile Val Lys Leu Leu Ala Thr Ile Leu Gln Gly Gln Phe Pro His Thr 
                965                 970                 975     


Lys Ile Ile Glu Val Arg Gln Glu Gln Ile Ala Asn Ile Arg Tyr Gln 
            980                 985                 990         


Leu Asp Ile Tyr Arg Leu Arg Asp  Leu Asn Val Tyr Tyr  Arg Gly Met 
        995                 1000                 1005             


Asp Ala  Tyr Leu Ala Ala Val  Val Gly Thr Tyr Leu  Tyr Thr Val 
    1010                 1015                 1020             


Tyr Pro  Lys Ala Arg Arg Phe  Phe Val Tyr Gly Lys  Tyr Leu Lys 
    1025                 1030                 1035             


Gln Asp  Lys Asn Asn Arg Glu  Glu Gly Gln Phe Lys  Thr Ser Gly 
    1040                 1045                 1050             


His Ser  Phe Asn Phe Leu Trp  Lys Leu Phe Tyr Gly  Gly Glu Asp 
    1055                 1060                 1065             


Glu Ile  Tyr Val Gln Gly Thr  Asn Asn Ile Ala Phe  Asn Lys Lys 
    1070                 1075                 1080             


Asp Leu  Leu Gln Lys Ile Lys  Glu Val Asn Asn Tyr  Lys Phe Gln 
    1085                 1090                 1095             


Asn Val  Ser Leu Ala Thr Gly  Thr Arg Arg Ser Asp  Leu Phe Lys 
    1100                 1105                 1110             


Gln Thr  Ile Tyr Pro Arg Gln  Glu Arg Asp Val Lys  Lys Ser Arg 
    1115                 1120                 1125             


Thr Leu  Ile Thr Lys Lys Asn  Gly Leu Phe Thr Asp  Ile Tyr Gly 
    1130                 1135                 1140             


Gly Tyr  Thr Gly Asn Val Asn  Ser Tyr Phe Val Leu  Val Gln Thr 
    1145                 1150                 1155             


Lys Tyr  Lys Lys Asp Pro Asp  Lys Phe Asn Leu Tyr  Gly Val Pro 
    1160                 1165                 1170             


Asn Arg  Tyr Val Ala Met Leu  Glu Gln Ser Lys Ser  Lys Gly Asn 
    1175                 1180                 1185             


Tyr Glu  Thr Lys Leu Lys Glu  Val Met Glu Pro Ile  Ile Lys Asn 
    1190                 1195                 1200             


Ser Asn  Lys Arg Met Ile Gly  Tyr Lys Ile Leu Lys  Asp Arg Ile 
    1205                 1210                 1215             


Leu Tyr  Asn Gln Ile Ile Glu  Asp Asn Gly Tyr Lys  Phe Phe Ile 
    1220                 1225                 1230             


Thr Ser  Ser Gln Tyr Arg His  Asn Phe Arg Gln Met  Ile Leu Ser 
    1235                 1240                 1245             


Asp Gln  Ser Lys Lys Ile Leu  Met Asp Tyr Val Phe  Asp Pro Asn 
    1250                 1255                 1260             


Phe Tyr  Arg His Lys Lys Thr  Glu Asp Val Asn Asn  Asp Lys Asp 
    1265                 1270                 1275             


Ala Lys  Leu Ile Lys Val Tyr  Asp Glu Ile Leu Tyr  Gln Ile Asp 
    1280                 1285                 1290             


His Tyr  Leu Gln Ile Phe Thr  Ile Gly His His Ile  Asp Lys Leu 
    1295                 1300                 1305             


Lys Lys  Ala Arg Pro Leu Phe  Glu Lys Cys Thr Leu  Ala Glu Lys 
    1310                 1315                 1320             


Ala Gln  Val Leu Asn Asp Ile  Leu Ile Ile Ala Gly  Ser Asn Gln 
    1325                 1330                 1335             


Thr Glu  Leu Ala Leu Lys Pro  Leu Lys Thr Thr Lys  Leu Thr Phe 
    1340                 1345                 1350             


Ser Lys  Gln Asn Cys Pro Leu  Thr Lys Asp Ala Ile  Phe Tyr Tyr 
    1355                 1360                 1365             


Gln Ser  Pro Thr Gly Met Ile  Thr Arg Lys Glu Ser  Ile Lys Asp 
    1370                 1375                 1380             


Ile Leu  Lys Gln Asn Ile 
    1385                 


<210>  1125
<211>  1390
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1125

Met Ser Arg Lys Asn Ile Val Glu Asp Tyr Ala Ile Ser Leu Asp Ile 
1               5                   10                  15      


Gly Asn Ala Ser Val Gly Trp Val Ala Phe Thr Pro Asn Tyr Arg Leu 
            20                  25                  30          


Val Arg Ala Lys Gly His Glu Leu Ile Gly Ala Arg Leu Phe Glu Pro 
        35                  40                  45              


Ala Ser Thr Ala Glu Glu Arg Arg Met Ala Arg Thr Thr Arg Arg Arg 
    50                  55                  60                  


Tyr Ser Arg Arg Arg Trp Arg Leu Arg Met Leu Asp Ser Met Phe Asp 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Val Asp Pro Ser Phe Leu Met Arg Arg Lys Tyr 
                85                  90                  95      


Ser Trp Val His Pro Glu Asp Lys Asn Asn Glu Ala His Trp Phe Gly 
            100                 105                 110         


Gly Val Leu Phe Asp Ser Asn Glu Gln Asp Lys Arg Phe Tyr Glu Lys 
        115                 120                 125             


Tyr Pro Thr Ile Tyr His Leu Arg Lys Ser Leu Met Glu Asp Asp Glu 
    130                 135                 140                 


Arg His Asp Ile Arg Glu Val Tyr Leu Ala Ile His His Met Met Lys 
145                 150                 155                 160 


Tyr Arg Gly Asn Phe Leu Thr Glu Gly Glu Leu Ala Pro Ser Ser Val 
                165                 170                 175     


Phe Asp Val Glu Asp Phe Leu Lys Leu Leu Thr Glu Ile Leu Gln Tyr 
            180                 185                 190         


Ala Asp Glu Gly Glu Thr Asp Glu Asp Leu Glu Leu Gln Ala Asp Ala 
        195                 200                 205             


Asn Gly Leu Thr Glu Ala Leu Cys Thr Thr Lys Gly Ser Arg Ser Ile 
    210                 215                 220                 


Arg Ala Lys Asn Ala Ile Ser Ile Leu Lys Glu Gly Ala Ala Leu Ser 
225                 230                 235                 240 


Lys Arg Gln Glu Ala Ile Ile Ser Ala Val Phe Ala Gly Leu Glu Gly 
                245                 250                 255     


Asn Lys Leu Asp Leu Thr Lys Val Phe Pro Asn Lys Glu Tyr Thr Pro 
            260                 265                 270         


Glu Asp Lys Lys Thr Leu Ala Leu Tyr Phe Asn Lys Ala Asp Tyr Asp 
        275                 280                 285             


Glu Lys Arg Val Ala Ile Gly Asp Ser Gly Leu Leu Glu Asp Asn Glu 
    290                 295                 300                 


Cys Asp Phe Leu Asp Cys Ile Arg Arg Gln Tyr Ser Ala Ile Ala Leu 
305                 310                 315                 320 


Lys Gln Leu Leu Gly Asp Phe Thr Ser Ile Ser Glu Ser Met Cys Ala 
                325                 330                 335     


Arg Tyr Glu Gln His Gln Arg Asn Trp Asp Leu Ile Lys Arg Tyr Cys 
            340                 345                 350         


Arg Glu Glu Ser Asn Ala Glu Asn Ile Asn Lys Asn Tyr Gly Ala Leu 
        355                 360                 365             


Val Gly Trp Glu Ile Val Gly Gly Gln Arg Arg Ser Ile Arg Gly Ala 
    370                 375                 380                 


Lys Asp Tyr Glu Asn Lys Arg Lys Ala Ala Asn Lys Phe Phe Glu Glu 
385                 390                 395                 400 


Leu Ile Lys Gly Ser Ala Leu Pro Asp Gln Glu Lys Glu Arg Leu Leu 
                405                 410                 415     


His Asp Ile Glu Asn Asp Glu Leu Phe Pro Val Gln Arg Asp Ser Gly 
            420                 425                 430         


Asn Gly Val Ile Pro Tyr Gln Leu His Leu Asn Glu Leu Lys Lys Ile 
        435                 440                 445             


Ile Gln Lys Gln Gly Arg Tyr Tyr Pro Phe Leu Leu Glu Thr Tyr Ile 
    450                 455                 460                 


Lys Asp Gly Lys Glu Val Asn Lys Leu Glu Gly Leu Leu Ala Phe Arg 
465                 470                 475                 480 


Val Pro Tyr Tyr Val Gly Pro Leu Val Ser Arg Asp Asp Met Gln Ser 
                485                 490                 495     


Ser Asp Asn Arg Glu Asn His Trp Met Ile Arg Arg Lys Lys Gly Val 
            500                 505                 510         


Ile Thr Pro Trp Asn Phe Asp Glu Met Val Asp Lys Asp Lys Ser Gly 
        515                 520                 525             


His Glu Phe Ile Lys Ser Leu Val Gly Thr Asp Ser Tyr Leu Leu Gly 
    530                 535                 540                 


Glu Thr Thr Leu Pro Lys Asn Ser Leu Leu Tyr Gln Glu Phe Glu Val 
545                 550                 555                 560 


Leu Asn Glu Leu Asn Asn Val Arg Leu Ser Ile Arg Thr Gly Asn His 
                565                 570                 575     


Trp Ala Asp Lys Arg Arg Val Arg Leu Gly His Asn Glu Lys Lys Leu 
            580                 585                 590         


Leu Leu Glu Glu Leu Phe Ala Lys Gln Ala Thr Val Thr Lys Lys Ala 
        595                 600                 605             


Ala Glu Asn Leu Leu Arg Arg Thr Tyr Gly Asn Thr Tyr Glu Ile Ser 
    610                 615                 620                 


Gly Leu Ala Asp Glu Lys Lys Phe Val Ser Ser Leu Ser Ser Tyr Gly 
625                 630                 635                 640 


Lys Met Cys Arg Thr Leu Lys Ala Ser Tyr Val Asn Ser His Lys Asp 
                645                 650                 655     


Val Val Glu Lys Ile Ile Glu Leu Gln Thr Val Phe Glu Asp Lys Lys 
            660                 665                 670         


Thr Leu Lys His Gln Leu Gln Leu Leu Gly Glu Leu Ala Glu Thr Asn 
        675                 680                 685             


Cys Glu Leu Leu Ser Asn Thr His Tyr Thr Gly Trp Gly Lys Leu Ser 
    690                 695                 700                 


Arg Lys Leu Leu Thr Ser Lys Val Val Val Cys Lys Ile Gly Asn Asp 
705                 710                 715                 720 


Phe Ala Pro Ala Lys His Ser Ile Ile Glu Ile Met Arg Glu Thr Asp 
                725                 730                 735     


Arg Asn Phe Met Glu Ile Ile Thr Asp Lys Asp Leu Gly Val Gly Glu 
            740                 745                 750         


Trp Ile Asn Gln Gln Asn Val Gly Ala Glu Asp Gly Asn Ser Tyr Gln 
        755                 760                 765             


Asp Ile Ile Asp Asp Leu Arg Val Ser Pro Lys Val Lys Arg Gly Ile 
    770                 775                 780                 


Asn Gln Ala Ile Arg Val Ile Asp Asp Ile Ser Lys Ala Val Gly Lys 
785                 790                 795                 800 


Glu Pro Ser Arg Ile Phe Leu Glu Leu Ala Gly Asp Val Gln Gln Ser 
                805                 810                 815     


Ala Arg Thr Val Ser Arg Lys Ser Arg Leu Gln Gly Leu Tyr Lys Ser 
            820                 825                 830         


Ala Gly Leu Gln Lys Glu Phe Arg Asp Leu Ala Glu Ser Leu Asn Glu 
        835                 840                 845             


Cys Ser Asp Asn Asp Leu Arg Asp Asp Arg Leu Phe Leu Tyr Tyr Thr 
    850                 855                 860                 


Gln Leu Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asn Leu Asp Arg 
865                 870                 875                 880 


Leu Ser Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Val Thr 
                885                 890                 895     


Gln Asn Asp Ser Leu Asp Asn Arg Val Leu Val Ala Arg Val Ala Asn 
            900                 905                 910         


Ala Arg Lys Thr Asp Ser Phe Gln Tyr Leu Pro Glu Ile Ala Asn Arg 
        915                 920                 925             


Met Arg Asn Phe Trp Gln Glu Leu Leu Asp Ala Gly Leu Ile Ser Arg 
    930                 935                 940                 


Val Lys Phe Asp Arg Leu Asn Arg Gln Asn Asp Phe Ser Ser His Glu 
945                 950                 955                 960 


Lys Glu Arg Phe Val Gln Arg Ser Leu Val Glu Thr Arg Gln Ile Met 
                965                 970                 975     


Lys Asn Val Ala Thr Ile Met Arg Lys Arg Tyr Gly Asn Ser Ala Ala 
            980                 985                 990         


Val Ile Gly Leu Asn Ser Glu Leu  Thr Lys Glu Met Arg  Gln Tyr Leu 
        995                 1000                 1005             


Gly Phe  Ser His Lys Asn Arg  Asp Ile Asn Asp Tyr  His His Ala 
    1010                 1015                 1020             


Gln Asp  Ala Leu Cys Leu Gly  Ile Ala Gly Gln Phe  Ala Val Asn 
    1025                 1030                 1035             


Arg Gly  Phe Phe Ala Asn Gly  Met Val Ser Asp Gly  Ala Val Asn 
    1040                 1045                 1050             


Ser Tyr  Asn Leu Tyr Leu Gln  Asp Tyr Leu Arg Gly  Tyr Arg Glu 
    1055                 1060                 1065             


Lys Met  Ser Ala Gly Glu Arg  Arg Arg Asn Lys Ala  Phe Gly Phe 
    1070                 1075                 1080             


Ile Val  Gly Ser Met Asn Ser  Gln Asp Ser Asp Lys  Arg Ile Asn 
    1085                 1090                 1095             


Pro Lys  Thr Gly Glu Ile Ala  Trp Asp Asp Glu Asp  Lys Val Tyr 
    1100                 1105                 1110             


Leu Arg  Lys Val Met Asn Tyr  Arg Lys Met Leu Ile  Thr Gln Lys 
    1115                 1120                 1125             


Val Gly  Asp Ser Phe Gly Ala  Leu Tyr Asn Glu Thr  Arg Tyr Gly 
    1130                 1135                 1140             


Thr Ser  Ala Lys Glu Gly Val  Asn Gly Ile Pro Phe  Asp Lys His 
    1145                 1150                 1155             


Lys Gln  Asp Thr Ala Leu Tyr  Gly Gly Phe Ser Ser  Ala Lys Thr 
    1160                 1165                 1170             


Val Tyr  Val Val Leu Ile Glu  Ala Lys Lys Lys Thr  Arg Leu Val 
    1175                 1180                 1185             


Asn Ile  Thr Met Gln Glu Tyr  Ala Glu Leu Gly Asp  His Pro Ser 
    1190                 1195                 1200             


Asp Asp  Ala Leu Arg Ala Val  Leu Ala Lys Lys Lys  Pro Glu Tyr 
    1205                 1210                 1215             


Gly Lys  Ala Arg Ile Leu Leu  Arg His Ile Pro Pro  Met Gln Leu 
    1220                 1225                 1230             


Ile Arg  Tyr Gly Gly Ala Leu  Val Thr Val Lys Ser  Ala Thr Glu 
    1235                 1240                 1245             


Leu Asn  Asn Ala Gln Gln Leu  Trp Leu Pro Tyr Lys  Glu Tyr Cys 
    1250                 1255                 1260             


Leu Phe  Asp Asp Val Ile Ser  Ser Ala Ile Asp Val  Asp Glu Ala 
    1265                 1270                 1275             


Arg Leu  Cys Gln Met Leu Asp  Val Ile Leu Asp Ala  Ile Gln Lys 
    1280                 1285                 1290             


Tyr Tyr  Pro Lys Gln Arg Phe  Asp Glu Thr Gly Leu  Ser Ala Phe 
    1295                 1300                 1305             


His Asn  Ala Phe Asn Glu Val  Ser Ala Glu Asp Lys  Lys Arg Ile 
    1310                 1315                 1320             


Val Gly  Glu Met Val Gly Met  Leu His Ala Asp Ala  Lys Asn Ala 
    1325                 1330                 1335             


Asn Leu  Lys Ala Leu Gly Met  Ser Gly Arg Trp Gly  Arg Met Ser 
    1340                 1345                 1350             


Asn Gln  Ser Gly Tyr Thr Phe  Ala Asp Ser Asp Glu  Phe Ile Phe 
    1355                 1360                 1365             


Gln Ser  Pro Ser Gly Leu Phe  Glu Lys Arg Ile Ser  Val Gly Glu 
    1370                 1375                 1380             


Leu Lys  Lys Arg Arg Ala Arg  
    1385                 1390 


<210>  1126
<211>  1390
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1126

Met Lys Glu Val Lys Asp Tyr Ile Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Cys Gly Tyr Val Val Thr Asp Lys Gln Asn Asn Ile Leu Lys Leu 
            20                  25                  30          


Lys Gly Lys Thr Ala Ile Gly Ala Arg Leu Phe Lys Glu Gly Glu Ala 
        35                  40                  45              


Ala Ala Asp Arg Arg Ala Phe Arg Thr Thr Arg Arg Arg Leu Ala Arg 
    50                  55                  60                  


Arg Arg Trp Arg Leu Gly Leu Leu Glu Glu Ile Phe Asp Glu Glu Met 
65                  70                  75                  80  


Ala Lys Val Asp Ser Ser Phe Phe Arg Arg Leu Lys Glu Ser Asp Tyr 
                85                  90                  95      


Ser Pro Lys Asp Ser Arg Lys Gln Phe Asn Ser Ile Val Phe Glu Ser 
            100                 105                 110         


Ser Glu Ala Asp Lys Asp Phe Tyr Asp Lys Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Asn Ala Leu Met His Asp Asp Gln Lys His Asp Leu Arg Glu 
    130                 135                 140                 


Ile Phe Leu Ala Val His His Ile Val Lys Tyr Arg Gly Asn Phe Leu 
145                 150                 155                 160 


Arg Glu Asp Ser Val Lys Ala Phe Lys Ala Ala Lys Phe Thr Leu Gln 
                165                 170                 175     


Gly Glu Asp Gly Ile Gly Pro Val Asp Lys Leu Asn Asp Leu Leu Lys 
            180                 185                 190         


Glu Ile Tyr Ile Glu Ala Ala Pro Glu Leu Ala Thr Asp Asn Leu Ser 
        195                 200                 205             


Lys Ile Glu Glu Ile Ile Lys Asp Lys Lys Leu Tyr Lys Gln Asp Lys 
    210                 215                 220                 


Leu Lys Gln Val Ala Thr Leu Leu Thr Lys Glu Ala Asp Thr Lys Asp 
225                 230                 235                 240 


Lys Ala Lys Leu Asn Lys Asp Val Ala Lys Gln Val Ala Asn Ala Phe 
                245                 250                 255     


Met Gly Tyr Met Ile Ile Phe Asp Thr Ile Phe Asn Leu Ser Asp Val 
            260                 265                 270         


Asp Ser Lys Asp Tyr Lys Val Lys Phe Ser Asp Ala Asn Ile Asp Glu 
        275                 280                 285             


Lys Leu Asp Thr Leu Thr Ser Leu Leu Thr Asp Lys Gln Thr Glu Phe 
    290                 295                 300                 


Val Leu Glu Leu Gln Asn Ile Tyr Asn Thr Ile Val Leu Asn Glu Ile 
305                 310                 315                 320 


Val Pro Asp Gly Lys Ser Leu Ser Glu Ser Met Val Lys Lys Tyr Asp 
                325                 330                 335     


Asp His Lys Glu Asp Leu Lys Leu Phe Lys Ala Tyr Leu Asn Ser Leu 
            340                 345                 350         


Thr Asp Val Lys Lys Val Lys Glu Leu Lys Ala Ala Tyr Val Leu Tyr 
        355                 360                 365             


Val Asn Asn Arg Lys Ala Asn Leu Leu Ala Ala Lys Lys Leu Leu Gly 
    370                 375                 380                 


Lys Lys Ala Asp Asn Met Asn Asn Phe Glu Ile Phe Ser Lys Phe Val 
385                 390                 395                 400 


Ala Asp Asn Leu Asp Asp Ser Glu Leu Ala Asn Lys Ile Arg Ala Arg 
                405                 410                 415     


Leu Asp Leu Gly Glu Phe Leu Pro Lys Gln Arg Thr Asn Gln Asn Gly 
            420                 425                 430         


Val Ile Pro Tyr Gln Leu His Gln Val Glu Leu Thr Gln Ile Leu Glu 
        435                 440                 445             


Lys Gln Gly Lys Tyr Tyr Pro Phe Leu Ile Ala Pro Asn Pro Val Glu 
    450                 455                 460                 


Ser His Arg Asn Asn Ala Pro Tyr Arg Ile Ser Glu Leu Val Ser Phe 
465                 470                 475                 480 


Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Asp Pro Asn Asn Ala Glu 
                485                 490                 495     


Ser Glu Gln Asp Lys Ala Asn Ser Ser Phe Ala Trp Met Val Arg Gln 
            500                 505                 510         


Lys Gln Gly Gln Ile Thr Pro Trp Asn Phe Glu Glu Met Val Asp Thr 
        515                 520                 525             


Thr Glu Ser Ala Asn Lys Phe Ile Lys Arg Met Thr Thr Lys Asp Thr 
    530                 535                 540                 


Tyr Leu Leu Gly Glu Asp Val Leu Pro Ala Ser Ser Leu Ile Tyr Gln 
545                 550                 555                 560 


Lys Phe Val Ile Leu Asn Glu Leu Asn Asn Ile Lys Val Asn Gly Ile 
                565                 570                 575     


Lys Leu Thr Ser Glu Glu Lys His Gln Ile Phe Asp Glu Leu Phe Lys 
            580                 585                 590         


Val Gln Lys Ser Val Ser Leu Lys Asn Leu Val Asn Phe Leu Thr Ala 
        595                 600                 605             


His Gly Cys Val Ala Pro Asn Val Thr Gly Leu Ser Asp Gly Thr Lys 
    610                 615                 620                 


Phe Asn Ser Ser Tyr Ser Thr Tyr Ile Asp Tyr Arg Lys Ile Phe Gly 
625                 630                 635                 640 


Ala Glu Ile Asp Asn Lys Asp Lys Gln Ala Asp Phe Glu Lys Met Ile 
                645                 650                 655     


Glu Trp Ser Thr Val Phe Glu Asp Arg Lys Ile Phe Lys Arg Lys Leu 
            660                 665                 670         


Glu Glu Ile Thr Trp Leu Thr Pro Asp Gln Ile Lys Gln Val Ser Ser 
        675                 680                 685             


Lys Arg Tyr Ser Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr Gln 
    690                 695                 700                 


Ile Thr Asp Glu Asn Gly Val Asn Ile Leu Gln Arg Leu Trp Asn Glu 
705                 710                 715                 720 


Ser Glu Asn Leu Met Gln Ile Leu Ser Asp Pro Val Ile Lys Glu Lys 
                725                 730                 735     


Ile Val Ala Ala Asn Ser Ser Phe Thr Lys Ala Thr Asp Met Glu Asp 
            740                 745                 750         


Ile Leu Ala Glu Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg 
        755                 760                 765             


Gln Val Met Arg Val Val Asp Asp Ile Ile Ala Ala Ala His Gly Lys 
    770                 775                 780                 


Lys Pro Ser Gln Ile Ala Ile Glu Phe Ala Arg Glu Asp Gln Asp Asn 
785                 790                 795                 800 


Pro Gln Met Thr Lys Ser Arg Lys Ala Gln Leu Asp Gln Ile Tyr Ala 
                805                 810                 815     


Lys Ile Ser Asp Glu Phe Leu Asp Asn Ala Val Lys Asn Glu Leu Lys 
            820                 825                 830         


Asn Met Lys Asp Asn Lys Pro Leu Ala Lys Asp Lys Val Phe Leu Tyr 
        835                 840                 845             


Phe Lys Gln Met Gly Arg Asp Ala Tyr Thr Gly Glu Lys Leu Ser Leu 
    850                 855                 860                 


Asp Asn Leu Gln Asp Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Phe 
865                 870                 875                 880 


Ile Lys Asp Asp Ser Phe Asp Asn Arg Val Leu Val Gln Arg Ala Val 
                885                 890                 895     


Asn Asn Gly Lys Ser Asp Asn Val Pro Val Leu Leu Phe Gly Asn Lys 
            900                 905                 910         


Gln Ala Ala Asp Leu Gly Met Thr Ile Arg Gln Met Trp Lys Lys Trp 
        915                 920                 925             


Leu Glu Leu Gly Leu Ile Ser Lys Arg Lys Phe Asn Asn Leu Ile Thr 
    930                 935                 940                 


Asp Pro Glu Lys Ile Ser Ser Tyr Met Ala Ser Gly Phe Val Asn Arg 
945                 950                 955                 960 


Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Val Ala Asn Ile Leu 
                965                 970                 975     


Gln Ala Lys Leu Pro Asp Thr Glu Ile Ile Glu Val Lys Ala Ser Tyr 
            980                 985                 990         


Asn Ser Ile Leu Arg Lys Glu Phe  Asn Leu Tyr Lys Ser  Arg Glu Val 
        995                 1000                 1005             


Asn Asp  Tyr His His Ala Ile  Asp Ala Tyr Leu Thr  Thr Ile Val 
    1010                 1015                 1020             


Gly Asn  Tyr Leu Tyr Gln Val  Tyr Pro Lys Leu Arg  Pro Tyr Phe 
    1025                 1030                 1035             


Val Tyr  Gly Gln Phe Lys Lys  Phe Gly Lys Ala Glu  Ser Lys Glu 
    1040                 1045                 1050             


Asp Asp  Pro Val Lys Lys Ile  Lys Gln Phe Asn Phe  Ile Tyr Gln 
    1055                 1060                 1065             


Leu Leu  Trp Gly Lys Asp Asp  Ala Ile Cys Ile Ser  His Thr Asp 
    1070                 1075                 1080             


Gln Gln  Val Phe Ser Arg Ser  Asp Ile Ile Asn Lys  Leu Thr Thr 
    1085                 1090                 1095             


Ala Tyr  Asn Phe Lys Tyr Met  Asn Val Ser Gln Glu  Val Tyr Thr 
    1100                 1105                 1110             


Asn Ser  Asn Asn Val Phe Lys  Gln Thr Val Phe Pro  Ile Ile Glu 
    1115                 1120                 1125             


Arg Asp  Thr Ala Lys Ser Arg  Ser Leu Ile Pro Lys  Lys Leu Asp 
    1130                 1135                 1140             


Arg Pro  Thr Asp Ile Tyr Gly  Gly Tyr Ser Asn Asn  Asn Asp Ala 
    1145                 1150                 1155             


Tyr Leu  Ala Leu Val Lys Val  Asp Lys Lys Lys Gly  Ser Glu Tyr 
    1160                 1165                 1170             


Arg Val  Val Gly Ile Pro Met  Arg Ala Ala Ala Met  Leu Lys Ala 
    1175                 1180                 1185             


Ser Lys  Asn Tyr Asp Glu Asp  Leu Arg Lys Val Ile  Glu Pro Met 
    1190                 1195                 1200             


Val Met  Leu Asp Lys Asn Gly  Lys Ala Lys Arg Gly  Ile Glu Gly 
    1205                 1210                 1215             


Phe Arg  Val Leu Val Gly Lys  Met Pro Phe Lys Gln  Ala Val Leu 
    1220                 1225                 1230             


Asp Gly  Asp Lys Lys Phe Met  Leu Thr Gly Thr Asn  Asp Met Pro 
    1235                 1240                 1245             


Asn Leu  Lys Gln Leu Val Leu  Ser Gln Gln Asn Met  Lys Tyr Ile 
    1250                 1255                 1260             


Leu Asp  Tyr Ile Asp Asp Ile  Asp Cys Arg Lys His  Lys Phe Gly 
    1265                 1270                 1275             


Asn Lys  Thr Gln Asp Gly Asn  Lys Cys Leu Leu Glu  Val Phe Asp 
    1280                 1285                 1290             


Glu Ile  Ile Gln Ile Val Asp  Lys His Phe Glu Leu  Phe Asp Asn 
    1295                 1300                 1305             


Arg Ser  Phe Arg Glu Lys Leu  Arg Asn Ser Arg Val  Asn Phe Ile 
    1310                 1315                 1320             


Asn Leu  Ala Leu Ser Lys Lys  Ala Glu Ala Leu Leu  Ala Ile Leu 
    1325                 1330                 1335             


Asn Gly  Leu His Ala Asn Ser  Met Arg Thr Thr Ile  Lys Glu Leu 
    1340                 1345                 1350             


Lys Ile  Ser Asp Leu Gly Arg  Ile Gln Ser Ser Val  Ile Ser Pro 
    1355                 1360                 1365             


Glu Ala  Gln Leu Ile Tyr Gln  Ser Pro Thr Gly Leu  Phe Glu Arg 
    1370                 1375                 1380             


Arg Val  Lys Val Lys Asp Leu  
    1385                 1390 


<210>  1127
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1127

Met Glu Asn Lys Gln Tyr Tyr Ile Gly Leu Asp Val Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Thr Asp Thr Ser Tyr Asn Leu Leu Arg Ala Lys 
            20                  25                  30          


Gly Lys Asp Met Trp Gly Ala Arg Leu Phe Glu Lys Ala Asn Thr Ala 
        35                  40                  45              


Ala Glu Arg Arg Thr Lys Arg Thr Ser Arg Arg Arg Ser Glu Arg Glu 
    50                  55                  60                  


Lys Ala Arg Lys Ala Met Leu Lys Glu Leu Phe Ala Asp Glu Ile Asn 
65                  70                  75                  80  


Arg Val Asp Pro Ser Phe Phe Ile Arg Leu Glu Glu Ser Lys Phe Phe 
                85                  90                  95      


Leu Asp Asp Arg Ser Glu Asn Asn Arg Gln Arg Tyr Thr Leu Phe Asn 
            100                 105                 110         


Asp Ala Thr Phe Thr Asp Lys Asp Tyr Tyr Glu Lys Tyr Lys Thr Ile 
        115                 120                 125             


Phe His Leu Arg Ser Ala Leu Ile Asn Ser Asp Glu Lys Phe Asp Val 
    130                 135                 140                 


Arg Leu Val Phe Leu Ala Ile Leu Asn Leu Phe Ser His Arg Gly His 
145                 150                 155                 160 


Phe Leu Asn Ala Ser Leu Lys Gly Asp Gly Asp Ile Gln Gly Met Asp 
                165                 170                 175     


Val Phe Tyr Asn Asp Leu Val Glu Ser Cys Glu Tyr Phe Glu Ile Glu 
            180                 185                 190         


Leu Pro Arg Ile Thr Asn Ile Asp Asn Phe Glu Lys Ile Leu Ser Gln 
        195                 200                 205             


Lys Gly Lys Ser Arg Thr Lys Ile Leu Glu Glu Leu Ser Glu Glu Leu 
    210                 215                 220                 


Ser Ile Ser Lys Lys Asp Lys Ser Lys Tyr Asn Leu Ile Lys Leu Ile 
225                 230                 235                 240 


Ser Gly Leu Glu Ala Ser Val Val Glu Leu Tyr Asn Ile Glu Asp Ile 
                245                 250                 255     


Gln Asp Glu Asn Lys Lys Ile Lys Ile Gly Phe Arg Glu Ser Asp Tyr 
            260                 265                 270         


Glu Glu Ser Ser Leu Lys Val Lys Glu Ile Ile Gly Asp Glu Tyr Phe 
        275                 280                 285             


Asp Leu Val Glu Arg Ala Lys Ser Val His Asp Met Gly Leu Leu Ser 
    290                 295                 300                 


Asn Ile Ile Gly Asn Ser Lys Tyr Leu Cys Glu Ala Arg Val Glu Ala 
305                 310                 315                 320 


Tyr Glu Asn His His Lys Asp Leu Leu Lys Ile Lys Glu Leu Leu Lys 
                325                 330                 335     


Lys Tyr Asp Lys Lys Ala Tyr Asn Asp Met Phe Arg Lys Met Thr Asp 
            340                 345                 350         


Lys Asn Tyr Ser Ala Tyr Val Gly Ser Val Asn Ser Asn Ile Ala Lys 
        355                 360                 365             


Glu Arg Arg Ser Val Asp Lys Arg Lys Ile Glu Asp Leu Tyr Lys Tyr 
    370                 375                 380                 


Ile Glu Asp Thr Ala Leu Lys Asn Ile Pro Asp Asp Asn Lys Asp Lys 
385                 390                 395                 400 


Ile Glu Ile Leu Glu Lys Ile Lys Leu Gly Glu Phe Leu Lys Lys Gln 
                405                 410                 415     


Leu Thr Ala Ser Asn Gly Val Ile Pro Asn Gln Leu Gln Ser Arg Glu 
            420                 425                 430         


Leu Arg Ala Ile Leu Lys Lys Ala Glu Asn Tyr Leu Pro Phe Leu Lys 
        435                 440                 445             


Glu Lys Gly Glu Lys Asn Leu Thr Val Ser Glu Met Ile Ile Gln Leu 
    450                 455                 460                 


Phe Glu Phe Gln Ile Pro Tyr Tyr Val Gly Pro Leu Asp Lys Asn Pro 
465                 470                 475                 480 


Lys Lys Asp Asn Lys Ala Asn Ser Trp Ala Lys Ile Lys Gln Gly Gly 
                485                 490                 495     


Arg Ile Leu Pro Trp Asn Phe Glu Asp Lys Val Asp Val Lys Gly Ser 
            500                 505                 510         


Arg Lys Glu Phe Ile Glu Lys Met Val Arg Lys Cys Thr Tyr Ile Ser 
        515                 520                 525             


Asp Glu His Thr Leu Pro Lys Gln Ser Leu Leu Tyr Glu Lys Phe Met 
    530                 535                 540                 


Val Leu Asn Glu Ile Asn Asn Ile Lys Ile Asp Gly Glu Lys Ile Ser 
545                 550                 555                 560 


Val Glu Ala Lys Gln Lys Ile Tyr Asn Asp Leu Phe Val Lys Gly Lys 
                565                 570                 575     


Lys Val Ser Gln Lys Asp Ile Lys Lys Glu Leu Ile Ser Leu Asn Ile 
            580                 585                 590         


Met Asp Lys Asp Ser Val Leu Ser Gly Thr Asp Thr Val Cys Asn Ala 
        595                 600                 605             


Tyr Leu Ser Ser Ile Gly Lys Phe Thr Gly Val Phe Lys Glu Glu Ile 
    610                 615                 620                 


Asn Lys Gln Ser Ile Val Asp Met Ile Glu Asp Ile Ile Phe Leu Lys 
625                 630                 635                 640 


Thr Val Tyr Gly Asp Glu Lys Arg Phe Val Lys Glu Glu Ile Val Glu 
                645                 650                 655     


Lys Tyr Gly Asp Glu Ile Asp Lys Asp Lys Ile Lys Arg Ile Leu Gly 
            660                 665                 670         


Phe Lys Phe Ser Asn Trp Gly Asn Leu Ser Lys Ser Phe Leu Glu Leu 
        675                 680                 685             


Glu Gly Ala Asp Val Gly Thr Gly Glu Val Arg Ser Ile Ile Gln Ser 
    690                 695                 700                 


Leu Trp Glu Thr Asn Phe Asn Leu Met Glu Leu Leu Ser Ser Arg Phe 
705                 710                 715                 720 


Thr Tyr Met Asp Glu Leu Glu Lys Arg Val Lys Lys Leu Glu Lys Pro 
                725                 730                 735     


Leu Ser Glu Trp Thr Ile Glu Asp Leu Asp Asp Met Tyr Leu Ser Ser 
            740                 745                 750         


Pro Val Lys Arg Met Ile Trp Gln Ser Met Lys Ile Val Asp Glu Ile 
        755                 760                 765             


Gln Thr Val Ile Gly Tyr Ala Pro Lys Arg Ile Phe Val Glu Met Thr 
    770                 775                 780                 


Arg Ser Glu Gly Glu Lys Val Arg Thr Lys Ser Arg Lys Asp Arg Leu 
785                 790                 795                 800 


Lys Glu Leu Tyr Asn Gly Ile Lys Glu Asp Ser Lys Gln Trp Val Lys 
                805                 810                 815     


Glu Leu Asp Ser Lys Asp Glu Ser Tyr Phe Arg Ser Lys Lys Met Tyr 
            820                 825                 830         


Leu Tyr Tyr Leu Gln Lys Gly Arg Cys Met Tyr Ser Gly Glu Val Ile 
        835                 840                 845             


Glu Leu Asp Lys Leu Met Asp Asp Asn Leu Tyr Asp Ile Asp His Ile 
    850                 855                 860                 


Tyr Pro Arg Ser Phe Val Lys Asp Asp Ser Leu Asp Asn Leu Val Leu 
865                 870                 875                 880 


Val Lys Lys Glu Ile Asn Asn Arg Lys Gln Asn Asp Pro Ile Thr Pro 
                885                 890                 895     


Gln Ile Gln Ala Ser Cys Gln Gly Phe Trp Lys Ile Leu His Asp Gln 
            900                 905                 910         


Gly Phe Met Ser Asn Glu Lys Tyr Ser Arg Leu Thr Arg Lys Thr Gln 
        915                 920                 925             


Glu Phe Ser Asp Glu Glu Lys Leu Ser Phe Ile Asn Arg Gln Ile Val 
    930                 935                 940                 


Glu Thr Gly Gln Ala Thr Lys Cys Met Ala Gln Ile Leu Gln Lys Ser 
945                 950                 955                 960 


Met Gly Glu Asp Val Asp Val Val Phe Ser Lys Ala Arg Leu Val Ser 
                965                 970                 975     


Glu Phe Arg His Lys Phe Glu Leu Phe Lys Ser Arg Leu Ile Asn Asp 
            980                 985                 990         


Phe His His Ala Asn Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Ser 
        995                 1000                 1005             


Tyr Phe  Val Lys Phe Thr Arg  Asn Pro Ala Asn Phe  Ile Lys Asp 
    1010                 1015                 1020             


Ala Arg  Lys Asn Pro Asp Asn  Pro Val Tyr Lys Tyr  His Met Asp 
    1025                 1030                 1035             


Arg Phe  Phe Glu Arg Asp Val  Lys Ser Lys Ser Glu  Val Ala Trp 
    1040                 1045                 1050             


Ile Gly  Gln Ser Glu Gly Asn  Ser Gly Thr Ile Val  Ile Val Lys 
    1055                 1060                 1065             


Lys Thr  Met Ala Lys Asn Ser  Pro Leu Ile Thr Lys  Lys Val Glu 
    1070                 1075                 1080             


Glu Gly  His Gly Ser Ile Thr  Lys Glu Thr Ile Val  Gly Val Lys 
    1085                 1090                 1095             


Glu Ile  Lys Phe Gly Arg Asn  Lys Val Glu Lys Ala  Asp Lys Thr 
    1100                 1105                 1110             


Pro Lys  Lys Pro Asn Leu Gln  Ala Tyr Arg Pro Ile  Lys Thr Ser 
    1115                 1120                 1125             


Asp Glu  Arg Leu Cys Asn Ile  Leu Arg Tyr Gly Gly  Arg Thr Ser 
    1130                 1135                 1140             


Ile Ser  Ile Ser Gly Tyr Cys  Leu Val Glu Tyr Val  Lys Lys Arg 
    1145                 1150                 1155             


Lys Thr  Ile Arg Ser Leu Glu  Ala Ile Pro Val Tyr  Leu Gly Arg 
    1160                 1165                 1170             


Lys Asp  Ser Leu Ser Glu Glu  Lys Leu Leu Asn Tyr  Phe Arg Tyr 
    1175                 1180                 1185             


Asn Leu  Asn Asp Gly Gly Lys  Asp Ser Val Ser Asp  Ile Arg Leu 
    1190                 1195                 1200             


Cys Leu  Pro Phe Ile Ser Thr  Asn Ser Leu Val Lys  Ile Asp Gly 
    1205                 1210                 1215             


Tyr Leu  Tyr Tyr Leu Gly Gly  Lys Asn Asp Asp Arg  Ile Gln Leu 
    1220                 1225                 1230             


Tyr Asn  Ala Tyr Gln Leu Lys  Met Lys Lys Glu Glu  Val Glu Tyr 
    1235                 1240                 1245             


Ile Arg  Lys Ile Glu Lys Ala  Val Ser Met Ser Lys  Phe Asp Glu 
    1250                 1255                 1260             


Ile Asp  Arg Glu Lys Asn Pro  Val Leu Thr Glu Glu  Lys Asn Ile 
    1265                 1270                 1275             


Glu Leu  Tyr Asn Lys Ile Gln  Asp Lys Phe Glu Asn  Thr Val Phe 
    1280                 1285                 1290             


Ser Lys  Arg Met Ser Leu Val  Lys Tyr Asn Lys Lys  Asp Leu Ser 
    1295                 1300                 1305             


Phe Gly  Asp Phe Leu Lys Asn  Lys Lys Ser Lys Phe  Glu Glu Ile 
    1310                 1315                 1320             


Asp Leu  Glu Lys Gln Cys Lys  Val Leu Tyr Asn Ile  Ile Phe Asn 
    1325                 1330                 1335             


Leu Ser  Asn Leu Lys Glu Val  Asp Leu Ser Asp Ile  Gly Gly Ser 
    1340                 1345                 1350             


Lys Ser  Thr Gly Lys Cys Arg  Cys Lys Lys Asn Ile  Thr Asn Tyr 
    1355                 1360                 1365             


Lys Glu  Phe Lys Leu Ile Gln  Gln Ser Ile Thr Gly  Leu Tyr Ser 
    1370                 1375                 1380             


Cys Glu  Lys Asp Leu Met Thr  Ile 
    1385                 1390     


<210>  1128
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1128

Met Lys Glu Ile Lys Asp Tyr Ile Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Cys Gly Tyr Val Val Thr Asp Lys Gln Asn Asn Ile Leu Lys Leu 
            20                  25                  30          


Lys Gly Lys Thr Ala Ile Gly Ala Arg Leu Phe Lys Glu Gly Glu Ala 
        35                  40                  45              


Ala Ala Asp Arg Arg Ala Phe Arg Thr Thr Arg Arg Arg Leu Ala Arg 
    50                  55                  60                  


Arg Arg Trp Arg Leu Ser Leu Leu Glu Glu Ile Phe Asp Glu Glu Met 
65                  70                  75                  80  


Asn Lys Val Asp Pro Ser Phe Phe Arg Arg Leu Lys Glu Ser Asp Tyr 
                85                  90                  95      


Ser Pro Lys Asp Arg Arg Lys Gln Phe Asn Ser Ile Val Phe Glu Ser 
            100                 105                 110         


Ser Glu Ala Asp Lys Ala Phe Tyr Glu Lys Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Asn Ala Leu Met His Asp Asn Gln Lys His Asp Leu Arg Glu 
    130                 135                 140                 


Ile Phe Leu Ala Val His His Ile Val Lys Tyr Arg Gly Asn Phe Leu 
145                 150                 155                 160 


Arg Glu Asp Ser Val Lys Ala Phe Lys Ala Ala Lys Phe Thr Leu Gln 
                165                 170                 175     


Gly Glu Asp Gly Ile Gly Pro Val Asp Lys Leu Asn Asp Leu Leu Lys 
            180                 185                 190         


Glu Ile Tyr Ile Glu Ala Ala Pro Glu Leu Ala Thr Asp Asn Leu Ser 
        195                 200                 205             


Lys Ile Glu Asp Ile Ile Lys Asp Lys Lys Leu Tyr Lys Gln Asp Lys 
    210                 215                 220                 


Leu Lys Gln Ile Ala Asn Leu Leu Pro Lys Ala Val Asp Ser Lys Asp 
225                 230                 235                 240 


Lys Ala Lys Leu Asn Lys Asp Ile Ala Lys Gln Val Ala Asn Ala Leu 
                245                 250                 255     


Met Gly Tyr Met Phe Arg Leu Asp Thr Leu Phe Ser Phe Thr Asp Val 
            260                 265                 270         


Asp Val Lys Asp Tyr Lys Leu Lys Phe Ser Asp Ala Asn Ile Asp Glu 
        275                 280                 285             


Ser Leu Asp Ala Leu Thr Ser Leu Leu Thr Asp Ala Gln Ile Glu Phe 
    290                 295                 300                 


Val Leu Glu Leu Gln Ser Ile Tyr Asn Thr Ile Val Leu Asn Glu Ile 
305                 310                 315                 320 


Val Pro Asp Gly Met Ser Leu Ser Glu Ser Met Val Lys Lys Tyr Asp 
                325                 330                 335     


Asp His Lys Lys Asp Leu Lys Leu Tyr Lys Glu Tyr Ile Asp Ser Leu 
            340                 345                 350         


Ser Asp Gln Lys Lys Ala Lys Gln Leu Glu Ala Ala Tyr Ala Leu Tyr 
        355                 360                 365             


Val Asn Tyr Arg Lys Ala Asp Leu Leu Ala Ala Lys Lys Leu Phe Glu 
    370                 375                 380                 


Lys Lys Lys Ile Gly Asp Lys Lys Phe Val Asp Val Ile Ser Asn Phe 
385                 390                 395                 400 


Glu Val Phe Gly Lys Phe Val Ser Asp Asn Leu Asp Asp Ser Glu Leu 
                405                 410                 415     


Ala Asn Lys Ile Lys Ala Arg Leu Asp Leu Gly Glu Phe Leu Pro Lys 
            420                 425                 430         


Gln Arg Thr Asn Gln Asn Gly Val Ile Pro Tyr Gln Leu His Gln Val 
        435                 440                 445             


Glu Leu Thr Gln Ile Leu Glu Lys Gln Gly Lys Tyr Tyr Pro Phe Leu 
    450                 455                 460                 


Ile Thr Pro Asn Pro Val Glu Ser His Arg Asn Asn Ala Pro Tyr Glu 
465                 470                 475                 480 


Ile Ser Glu Leu Val Ser Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu 
                485                 490                 495     


Ile Asp Asn Gln Ser Ile Lys Asp Lys Gln Asn Lys Asn Lys Phe Ala 
            500                 505                 510         


Trp Met Val Arg Gln Lys Gln Gly Gln Ile Thr Pro Trp Asn Phe Glu 
        515                 520                 525             


Glu Met Val Asp Thr Thr Glu Ser Ala Asn Gln Phe Ile Lys Arg Met 
    530                 535                 540                 


Thr Arg Lys Asp Thr Tyr Leu Leu Ala Glu Asp Val Leu Pro Lys Ser 
545                 550                 555                 560 


Ser Leu Ile Tyr Gln Lys Phe Met Ile Leu Asp Glu Leu Asn Arg Ile 
                565                 570                 575     


Lys Ile Asp Gly Lys Lys Leu Thr Ser Lys Leu Lys His Asp Ile Phe 
            580                 585                 590         


Glu Lys Leu Phe Lys Lys Gln Lys Ser Ile Asn Leu Asp Asn Leu Lys 
        595                 600                 605             


Asn Tyr Leu Leu Ala Glu Gly Asn Ile Pro Gly Leu Ile Glu Gly Leu 
    610                 615                 620                 


Ser Asp Gly Ile Asn Phe Asn Asn Ser Phe Ser Thr Tyr Ile Asp Tyr 
625                 630                 635                 640 


Arg Asn Ile Phe Gly Asp Glu Ile Asp Asn Pro Asn Lys Gln Ala Asp 
                645                 650                 655     


Phe Glu Lys Met Ile Glu Trp Ser Thr Val Phe Glu Asp Arg Lys Ile 
            660                 665                 670         


Phe Lys Arg Lys Leu Lys Glu Ile Thr Trp Leu Thr Pro Glu Gln Ile 
        675                 680                 685             


Lys Gln Val Ser Ser Lys Arg Tyr Ser Gly Trp Gly Arg Leu Ser Lys 
    690                 695                 700                 


Lys Leu Leu Thr Gln Ile Thr Asp Glu Asn Gly Val Asn Ile Leu Gln 
705                 710                 715                 720 


Arg Leu Trp Asn Glu Pro Glu Thr Leu Thr Glu Val Leu Ala Asn Pro 
                725                 730                 735     


Val Ile Lys Arg Lys Ile Ser Glu Ala Asn Ser Leu Phe Val Gln Ile 
            740                 745                 750         


Asn Lys Val Glu Asn Ile Leu Asp Asp Ala Tyr Thr Ser Pro Gln Asn 
        755                 760                 765             


Lys Lys Ala Ile Arg Gln Val Ile Arg Val Val Asp Asp Ile Ile Ala 
    770                 775                 780                 


Ala Ala His Gly Lys Lys Pro Ser Gln Ile Ala Ile Glu Phe Ala Arg 
785                 790                 795                 800 


Ser Ser Gln Asn Lys Ala Lys Val Pro Asp Thr Arg Lys Lys Gln Leu 
                805                 810                 815     


Asp Lys Ile Tyr Asn Lys Ile Ser Ser Glu Ile Leu Asp Ser Ser Ile 
            820                 825                 830         


Lys Asn Glu Leu Lys Asn Leu Lys Ser Asn Lys Tyr Leu Ser Lys Asp 
        835                 840                 845             


Lys Leu Phe Leu Tyr Phe Lys Gln Met Gly Arg Asp Ala Tyr Thr Gly 
    850                 855                 860                 


Asp Lys Leu Ser Leu Asp Gln Leu Gln Asn Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Phe Pro Arg Ser Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu 
                885                 890                 895     


Thr Gln Lys Pro Ile Asn Ala Lys Lys Ser Asp Tyr Gly Ile Pro Ala 
            900                 905                 910         


Leu Glu Phe Gly Asn Lys Tyr Val Pro Asp Leu Gly Ile Thr Val Lys 
        915                 920                 925             


Glu Met Trp Lys Leu Trp Gln Glu Asn Gly Leu Ile Ser Lys Ser Lys 
    930                 935                 940                 


Leu Ile Asn Leu Cys Thr Asn Pro Lys Lys Ile Gly Ser Lys Arg Ala 
945                 950                 955                 960 


Ser Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys 
                965                 970                 975     


Leu Val Ala Ile Ile Leu Gln Ala Glu Phe Pro Asp Thr Glu Ile Ile 
            980                 985                 990         


Glu Val Lys Ala Leu Gln Asn Thr  Thr Leu Arg Glu Ser  Phe His Leu 
        995                 1000                 1005             


Tyr Lys  Asn Arg Ser Val Asn  Asp Tyr His His Ala  Ile Asp Ala 
    1010                 1015                 1020             


Tyr Leu  Thr Thr Ile Val Gly  Asn Tyr Leu Tyr Gln  Val Tyr Pro 
    1025                 1030                 1035             


Lys Leu  Arg Pro Tyr Phe Val  Tyr Gly Gln Phe Lys  Lys Phe Asn 
    1040                 1045                 1050             


Gln Glu  Lys Asn Ile Asp Ile  Leu Lys Arg Leu Lys  Asn Phe Asn 
    1055                 1060                 1065             


Phe Leu  Arg Gln Leu Ile Phe  Asn Thr Asp Asp Asn  Ile Tyr Ile 
    1070                 1075                 1080             


Ser Gly  Thr Lys Glu Ile Val  Phe Asn Lys Lys Asp  Ile Ile His 
    1085                 1090                 1095             


Lys Leu  Glu Thr Ala Tyr Gly  Tyr Lys Tyr Met Asn  Ile Ser Arg 
    1100                 1105                 1110             


Glu Cys  Tyr Gln Glu Thr Ser  Ser Leu Phe Asn Gln  Thr Leu Tyr 
    1115                 1120                 1125             


Ala His  Asn Ser Ser Val Lys  Asn Ser Leu Ile Pro  Lys Lys Lys 
    1130                 1135                 1140             


Gly Leu  Pro Thr Glu Ile Tyr  Gly Gly Tyr Ser Gly  Asn Lys Asp 
    1145                 1150                 1155             


Ser Phe  Phe Val Leu Val Lys  Ile Val Lys Lys Arg  Thr Asn Leu 
    1160                 1165                 1170             


Tyr Arg  Ile Val Gly Ile Pro  Thr Arg Glu Leu Ala  Lys Leu Asn 
    1175                 1180                 1185             


Ser Ser  Asn Asn Tyr Asn Gln  Ala Leu Asn Lys Ile  Val Glu Ser 
    1190                 1195                 1200             


Lys Leu  Cys Leu Lys Glu Thr  Glu Ser Phe Lys Ile  Leu Ile Lys 
    1205                 1210                 1215             


Arg Leu  Leu Tyr Gly Thr Leu  Ile Val Asp Asn Gly  Gln Lys Phe 
    1220                 1225                 1230             


Arg Ile  Gly Ser Phe Lys Glu  Lys His Asn Val Gln  Gln Leu Val 
    1235                 1240                 1245             


Leu Gln  Leu Lys Ser Met Lys  Tyr Ile Lys Phe Tyr  Ile Asp Gly 
    1250                 1255                 1260             


Gly Gln  Asn Tyr Phe Thr Asp  Val Glu Arg Lys Lys  Leu Glu Lys 
    1265                 1270                 1275             


Gln Asp  Arg Asp Lys Cys Leu  Leu Tyr Val Phe Asp  Asp Ile Met 
    1280                 1285                 1290             


Asn Val  Val Asn Lys Arg Phe  Thr Leu Phe Asp Met  Ser Lys Tyr 
    1295                 1300                 1305             


Glu Lys  Asp Gly Asp Ser Leu  Arg Glu Lys Phe Asn  Cys Leu Asp 
    1310                 1315                 1320             


Phe Asn  Asp Lys Val Ser Ile  Leu Ser Asp Leu Leu  Lys Ala Phe 
    1325                 1330                 1335             


His Ala  Asn Ser Asp Arg Thr  Ser Ile Thr Lys Leu  Lys Ile Thr 
    1340                 1345                 1350             


Asn Leu  Gly Arg His Gln Ala  Gly Lys Asn Gly Ile  Thr Leu Thr 
    1355                 1360                 1365             


Thr Asn  Ala Gln Ile Ile Tyr  Gln Ser Pro Thr Gly  Leu Phe Glu 
    1370                 1375                 1380             


Arg Arg  Ile Lys Ile Lys Asp  Leu 
    1385                 1390     


<210>  1129
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1129

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu His Glu His Glu Gln Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Ser Val Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Arg Pro Leu Ser Ile Asn Ala Asp Arg Thr Ser Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Phe Lys Leu Arg Arg Lys Asn 
65                  70                  75                  80  


Leu Ile Asn Leu Leu Leu Lys His His Ile Ile Ser Asn Glu Thr Ile 
                85                  90                  95      


Leu Thr Glu Val Gly Lys Asn Thr Thr His Gln Thr Leu Lys Leu Arg 
            100                 105                 110         


Ser Lys Ala Thr Gln Glu Lys Ile Ser Leu Glu Glu Phe Ala Arg Ile 
        115                 120                 125             


Leu Leu Ser Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Glu 
    130                 135                 140                 


Lys Asn Glu Asp Glu Gly Phe Thr Ile Asp Gly Met Ala Ile Ala Lys 
145                 150                 155                 160 


Glu Leu Tyr Asp Asn Gln Leu Thr Pro Gly Gln Tyr Val Tyr Asn Ile 
                165                 170                 175     


Leu Gln Asp Gly Lys Asn Tyr Ile Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asp Asp Val Trp Gln Phe Gln Lys Lys Phe Tyr Ser 
        195                 200                 205             


Ser Ile Leu Thr Asp Glu Phe Tyr Leu Lys Leu Lys Gly Gln Gly Lys 
    210                 215                 220                 


Glu Asn Thr Arg Lys Leu Phe Leu Ala Ile His Gln Val Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Lys Arg Asp Ala Val Lys Leu Gln Tyr Tyr Lys Trp 
                245                 250                 255     


Arg Ser Glu Ala Val Lys Lys Gln Leu Ser Ile Glu Glu Val Ala Tyr 
            260                 265                 270         


Val Leu Val Glu Ile Asn Asn Asn Ile Asn Lys Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Ala Leu Tyr Phe Asn Asn Glu Thr 
    290                 295                 300                 


Val Gly Glu Tyr Leu Tyr Gly Gln Leu Val Lys Asn Arg His Asn Ser 
305                 310                 315                 320 


Leu Arg Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Lys Ile Trp Glu Thr Gln Ala Lys Phe Tyr Asp Glu Leu Thr Asn Glu 
            340                 345                 350         


Leu Lys Gln Glu Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys His Leu Ile Ser Asp Cys Leu Phe Glu Lys His His 
    370                 375                 380                 


Lys Ala Ile Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp 
385                 390                 395                 400 


Gln Asn Leu Asn Tyr Leu Glu Phe Val His Thr Gln Ser Lys Glu Lys 
                405                 410                 415     


Ile Phe Phe Ser Glu Leu Asp Asp Glu Thr Arg Gln Phe Val Phe Ala 
            420                 425                 430         


Glu Leu Asn Ile Arg Gly Thr Leu Lys Ile Lys Glu Ile Lys Lys Leu 
        435                 440                 445             


Ile Gly Leu Glu Thr Pro Val Tyr Trp Lys Ser Asn Phe Asp Glu Ile 
    450                 455                 460                 


Glu Gly Asn Arg Thr Asn Gln Val Leu Tyr Asn Val Tyr Gln Asn Ile 
465                 470                 475                 480 


Ala Asp Asp Leu Gly Tyr Gly Phe Asp Trp Ser Lys Lys His Ala Phe 
                485                 490                 495     


Glu Ile Asn Ala Glu Leu Gln Ala Ile Phe Lys Asp Val Gly Ile Asp 
            500                 505                 510         


Thr Ala Ile Leu Asn Phe Asp Ala Ser Ile Glu Glu Asn Asp Phe Asp 
        515                 520                 525             


Lys Gln Lys Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Ala Glu Asp 
    530                 535                 540                 


Thr Asp Lys Ala Thr Glu Glu Asp Lys Leu Ile Tyr Gly Asn Ile Asn 
545                 550                 555                 560 


Thr Lys Leu Lys Lys Gln Leu His Gln Lys Tyr Gly Phe Glu Ala Lys 
                565                 570                 575     


Tyr Gly Asn Leu Leu Ala Asn Ile Ser Leu Gln Gln Asp Tyr Gly Asn 
            580                 585                 590         


Leu Ser Ala Lys Ala Ile Lys Lys Ile Leu Pro Tyr Leu Glu Ser Gly 
        595                 600                 605             


Asn Ser Phe Tyr Glu Ala Cys Thr Ile Val Gly Tyr Asn Pro Ser Asn 
    610                 615                 620                 


Ser Leu Thr Lys Glu Gln Gln Gln Asn Arg Lys Leu Lys Asp Lys Leu 
625                 630                 635                 640 


Glu Leu Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile 
                645                 650                 655     


Leu Asn Gln Lys Val Asn Leu Ile Asn Gln Leu Ile Asp Glu Tyr Gly 
            660                 665                 670         


Lys Pro Asp Glu Val Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Ser 
        675                 680                 685             


Ala Lys Gln Arg Ala Asp Leu Ser Lys His Ile Asn Asp Ala Thr Leu 
    690                 695                 700                 


Lys Asn Gln Asn Ile Arg Lys Thr Leu Gln Lys Asp Phe Gly Ile Gln 
705                 710                 715                 720 


Asn Pro Ser Lys Asn Asp Val Ile Arg Tyr Lys Leu Tyr Asp Glu Leu 
                725                 730                 735     


Lys Asp Asn Gly Tyr Lys Thr Leu Phe Thr Asn Lys Tyr Ile Pro Lys 
            740                 745                 750         


Glu Gln Leu Phe Ser Lys Asp Val Asp Ile Glu His Ile Ile Pro Lys 
        755                 760                 765             


Ala Ile Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Ala Tyr Arg 
    770                 775                 780                 


Asn Val Asn Leu Ala Lys Ala Asp Ala Thr Ala Phe Asp Phe Ile Ser 
785                 790                 795                 800 


Ser Lys His Leu Asp Asp Leu Asp Asn Tyr Lys Ala Arg Val Glu Thr 
                805                 810                 815     


Leu Phe Gln Ser Lys Arg Ile Ser Lys Gly Lys Tyr Lys Lys Leu Leu 
            820                 825                 830         


Met Thr Arg Gln Asn Leu Pro Glu Asp Phe Ile Glu Arg Asp Leu Arg 
        835                 840                 845             


Asn Ser Gln Tyr Ile Ala Lys Lys Ala Thr Ala Met Leu Lys Glu Val 
    850                 855                 860                 


Phe Arg Ser Val Val Pro Thr Ser Gly Arg Ile Thr Asp Lys Leu Arg 
865                 870                 875                 880 


Glu Asp Trp Asp Leu Val Asn Val Met Lys Glu Leu Asn Leu Pro Lys 
                885                 890                 895     


Tyr Arg Thr Leu Gly Leu Thr Glu Phe Ile Glu Arg Lys Asn Gly Asn 
            900                 905                 910         


Lys Ile Glu Gln Ile Ile Asp Trp Thr Lys Arg Asn Asp His Arg His 
        915                 920                 925             


His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Thr His Asn His Ile 
    930                 935                 940                 


Gln Tyr Leu Asn Asn Leu Asn Val Thr His Gly Leu Asn Gly Val Asp 
945                 950                 955                 960 


Thr Asp His Glu Lys Ser Arg Leu Tyr Ala Ile Lys Gln Ala Ile Thr 
                965                 970                 975     


Lys Val Tyr Ile Val Gly Lys Thr Lys Lys Arg Lys Phe Ile Pro Pro 
            980                 985                 990         


Met Glu Asn Phe Arg Ser His Ala  Lys Asn His Leu Glu  Gly Ile Leu 
        995                 1000                 1005             


Ile Ser  Phe Lys Asn Lys Asn  Lys Val Val Thr Lys  Asn Lys Asn 
    1010                 1015                 1020             


Arg Thr  Lys Thr Lys Thr Lys  Gly Val Phe Asn Glu  Lys Ile Gln 
    1025                 1030                 1035             


Leu Thr  Pro Arg Gly Gln Leu  His Lys Glu Thr Ile  Tyr Gly His 
    1040                 1045                 1050             


Ala Lys  Lys Val Ile Val Thr  Lys Glu Lys Val Ser  Ser Lys Phe 
    1055                 1060                 1065             


Thr His  Glu Lys Ile Leu Gln  Val Thr Lys Pro Ala  Tyr Arg Glu 
    1070                 1075                 1080             


Ala Leu  Leu Lys Arg Leu Ala  Ser Phe Lys Asn Asp  Pro Lys Lys 
    1085                 1090                 1095             


Ala Phe  Ser Gly Lys Asn Ala  Leu Ser Lys Asn Pro  Leu Tyr Phe 
    1100                 1105                 1110             


Asp Ile  Thr Ser Gly Lys Lys  Val Pro Asp Thr Val  Lys Leu Ser 
    1115                 1120                 1125             


Tyr Phe  Glu Asn Ser Tyr Val  Ile Arg Lys Glu Val  Asn Ser Asp 
    1130                 1135                 1140             


Asn Phe  Lys Asn Glu Lys Ser  Leu Gln Lys Val Leu  Asp Val Gly 
    1145                 1150                 1155             


Ile Arg  Arg Ile Leu Glu Lys  Arg Leu Ala Asp Tyr  Asn Gly Asn 
    1160                 1165                 1170             


Ala Lys  Glu Ala Phe Ser Asn  Leu Glu Lys Asn Pro  Ile Trp Lys 
    1175                 1180                 1185             


Asp Lys  Glu Lys Gly Val Cys  Ile Lys Lys Val Arg  Ile Glu Ala 
    1190                 1195                 1200             


Leu Lys  Asn Ala Glu Pro Leu  His Val Lys Lys Asp  His Leu Gly 
    1205                 1210                 1215             


Lys Ile  Ile Leu Asp Asp Glu  Asn Lys Ser Thr Pro  Val Asp Phe 
    1220                 1225                 1230             


Ile Ser  Thr Gly Asn Asn His  His Val Ala Ile Tyr  Glu Asp Lys 
    1235                 1240                 1245             


Asp Gly  Lys Leu His Asp Lys  Val Val Ser Phe Tyr  Glu Val Val 
    1250                 1255                 1260             


Ser Arg  Val Asn Leu Gly Leu  Pro Ile Ile Asp Lys  Ser Phe Asn 
    1265                 1270                 1275             


Ser His  Leu Asp Trp Lys Phe  Leu Phe Ser Met Lys  Gln Asn Glu 
    1280                 1285                 1290             


Met Phe  Val Phe Pro Ser Glu  Asp Phe Asn Pro Lys  Glu Ile Asp 
    1295                 1300                 1305             


Ile Gly  Asn Pro Lys Asn Asn  Glu Leu Ile Ser Lys  Tyr Leu Phe 
    1310                 1315                 1320             


Arg Val  Gln Lys Phe Ser Lys  Val Thr Tyr Gly Asn  Ser Ala Val 
    1325                 1330                 1335             


Arg Asp  Tyr Val Phe Arg His  His Leu Glu Thr Glu  Leu Ile Asp 
    1340                 1345                 1350             


Lys Lys  Glu Leu Lys Gly Ile  Thr Tyr Tyr Gln Leu  Lys Ser Ile 
    1355                 1360                 1365             


Gly Met  Leu Glu Lys Leu Val  Lys Val Arg Ile Asn  His Ile Gly 
    1370                 1375                 1380             


Lys Ile  Val His Ile Gly Glu  Tyr 
    1385                 1390     


<210>  1130
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1130

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Glu Ala Glu Asn Glu Asn Glu Val Ser Asp Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Gly Gly Lys Ala Tyr Ser Asn Asn Ala Glu Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Asn 
65                  70                  75                  80  


Leu Ile Glu Ile Leu Leu Lys Asn Gly Leu Ile Asn Lys Asp Thr Ala 
                85                  90                  95      


Leu Thr Glu Val Gly Lys Glu Thr Thr His Gln Thr Leu Lys Leu Arg 
            100                 105                 110         


Ser Lys Ser Ala Asn Glu Lys Val Ser Leu Glu Asp Leu Ala Lys Ile 
        115                 120                 125             


Leu Leu Ser Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Val 
    130                 135                 140                 


Asn Ala Glu Asp Asp Gly Val Ala Ile Asp Gly Met Glu Val Ala Lys 
145                 150                 155                 160 


Leu Leu Tyr Asp Glu Asn Met Thr Pro Gly Glu Tyr Val Leu Arg Leu 
                165                 170                 175     


Leu Glu Ser Gly Lys Lys His Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Glu Glu Phe Asn Leu Val Trp Lys Lys Gln Ser Glu Phe Tyr Pro 
        195                 200                 205             


Leu Ile Leu Thr Lys Glu Leu Phe Phe Glu Leu His Gly Lys Asn Lys 
    210                 215                 220                 


Ser Gln Thr Trp Ala Ile Cys Lys Glu Pro Leu Gly Ile Val Gly Val 
225                 230                 235                 240 


Lys Leu Lys Gly Ser Ala Lys Glu Gln Lys Phe Ala Arg Tyr Arg Leu 
                245                 250                 255     


Arg Lys Glu Ala Leu Thr Gln Gln Ile Glu Leu Glu His Leu Ala Ile 
            260                 265                 270         


Val Leu Gln Asp Ile Asn Asn Asp Ser Asn Lys Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Phe Phe Asn Lys Glu Thr 
    290                 295                 300                 


Val Gly Gln Tyr Gln Tyr Lys Gln Leu Gln Lys Asp Pro Asn Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Val Ile Trp Arg Asn Gln Ala Lys Phe Tyr Pro Gln Leu Thr Asn Glu 
            340                 345                 350         


Leu Lys Gln Glu Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys Asn Leu Ile Ser Phe Cys Gln Phe Glu Ser Thr Gln 
    370                 375                 380                 


Gln Asn Tyr Val Glu Lys Glu Thr Gly Lys Thr Lys Ile Arg Thr Ile 
385                 390                 395                 400 


Gly Arg Arg Val Ile Pro Arg Ser Ser Pro Leu Phe Gln Glu Phe Lys 
                405                 410                 415     


Ile Trp Gln Ser Leu Asn Asn Leu Lys Phe Val Asn Ser Glu Ser Asn 
            420                 425                 430         


Gln Ile Ile Gln Phe Ile Glu Leu Glu Asp Asp Val Lys Lys Val Val 
        435                 440                 445             


Phe Asp Glu Leu Asn Leu Arg Gly Asp Leu Lys Pro Asn Gln Val Leu 
    450                 455                 460                 


Lys Ile Leu Ser Lys Tyr Met Lys Ile Gly Lys Leu Thr Gln Trp Lys 
465                 470                 475                 480 


Cys Asn Leu Glu Glu Ile Glu Gly Asn Arg Thr Asn Ser Ala Leu Tyr 
                485                 490                 495     


Asn Val Phe Gln Met Ile Ala Glu Glu Glu Gly Tyr Gly Phe Asn Trp 
            500                 505                 510         


Gly Lys Lys Ser Ala Leu Glu Ile Lys Ser Glu Leu Lys Ala Ile Phe 
        515                 520                 525             


Ser Ser Ile Gly Ile Asn Asp Ser Ile Leu Asp Phe Asp Phe Ser Lys 
    530                 535                 540                 


Lys Leu Asp Gln Gln Leu Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser 
545                 550                 555                 560 


Ala Glu Glu Gly Asn Ser Lys Ile Thr Glu Glu Asp Lys Phe Thr Tyr 
                565                 570                 575     


Gly Asn Thr Gly Val Ala Leu Lys Lys Lys Leu His Leu Lys Phe Gly 
            580                 585                 590         


Phe Lys Pro Glu Tyr Ser Thr Leu Ile Ser Asn Leu Arg Phe Thr Ser 
        595                 600                 605             


Asp Tyr Gly Asn Leu Ser Ser Lys Ala Ile Ser Lys Ile Leu Pro Phe 
    610                 615                 620                 


Leu Arg Asp Gly Ser Asp Tyr Ser Lys Ala Cys Gln Tyr Ala Gly Tyr 
625                 630                 635                 640 


Asn His Ser Asn Ser Leu Ser Ala Asp Glu Leu Asp Asn Arg Glu Leu 
                645                 650                 655     


Lys Pro Lys Leu Glu Leu Leu Lys Lys Asn Ser Leu Arg Asn Pro Val 
            660                 665                 670         


Val Glu Lys Ile Leu Asn Gln Met Val Asn Val Val Asn Gln Val Ile 
        675                 680                 685             


Asp Asn Tyr Gly Lys Pro Asp Glu Val Arg Ile Glu Leu Ala Arg Glu 
    690                 695                 700                 


Leu Lys Lys Ser Ala Lys Glu Arg Ser Gln Met Thr Lys Asn Ile Ser 
705                 710                 715                 720 


Asp Ala Thr Arg Arg Asn Ala Glu Ile Lys Lys Tyr Leu Ser Lys Glu 
                725                 730                 735     


Phe Gly Ile Pro Asn Pro Thr Lys Gly Asp Val Ile Arg Tyr Arg Leu 
            740                 745                 750         


Trp Glu Glu Leu Ala Ser Asn Gly Tyr Lys Asp Leu Phe Arg Asp Lys 
        755                 760                 765             


Tyr Ile Pro Lys Glu Lys Leu Phe Ser Asn Glu Ile Asp Ile Glu His 
    770                 775                 780                 


Ile Ile Pro Lys Ala Leu Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr 
785                 790                 795                 800 


Leu Ala Phe Lys Val Asp Asn Leu Lys Lys Ala Asp Arg Thr Ala Phe 
                805                 810                 815     


Asp Phe Met Ser Thr Asp Phe Ser Lys Asp Val Asp Ser Tyr Ile Gly 
            820                 825                 830         


Arg Ile Glu Thr Leu His Lys Asp Gly Lys Ile Ser Ser Ser Lys Tyr 
        835                 840                 845             


Lys Lys Leu Met Ile Pro Lys Ser Lys Leu Val Asp Gly Phe Ile Glu 
    850                 855                 860                 


Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Gln Ala Arg Lys Met 
865                 870                 875                 880 


Leu Leu Glu Val Phe Lys Thr Val Val Pro Thr Thr Gly Lys Ile Thr 
                885                 890                 895     


Asp Lys Leu Arg Glu Asp Trp Asp Leu Ile Asn Val Met Lys Glu Leu 
            900                 905                 910         


Asn Phe Pro Lys Tyr Lys Ala Leu Gly Leu Thr Lys Lys Ile Glu Arg 
        915                 920                 925             


Phe Asp Val Gly Ser Glu Lys Ile Lys Ser Ile Asn Val Ile Glu Gly 
    930                 935                 940                 


Trp Ser Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Ala 
945                 950                 955                 960 


Val Ala Phe Thr Thr His Asn His Ile Gln Tyr Leu Asn Tyr Leu Asn 
                965                 970                 975     


Ala Arg Lys Asp Ile His His Glu Met His Gly Thr Ile Lys Ser Ile 
            980                 985                 990         


Glu Asn Gln Ile Ile Gly Lys Asp  Val Met Gly Lys Arg  Arg Phe Ile 
        995                 1000                 1005             


Pro Pro  Ile Ser Asp Leu Arg  Met Lys Ala Lys Leu  Gln Ile Glu 
    1010                 1015                 1020             


Ser Ile  Leu Ile Ser Phe Lys  Asn Lys Asn Lys Val  Val Thr Trp 
    1025                 1030                 1035             


Ser Val  Asn Lys Thr Lys Leu  Lys Gly Lys Asp Asn  Tyr Asn Lys 
    1040                 1045                 1050             


Arg Arg  Gln Leu Thr Pro Arg  Gly Gln Leu His Lys  Glu Thr Val 
    1055                 1060                 1065             


Tyr Gly  Lys Ile Lys Leu Ile  Asp Pro Lys Pro Leu  Lys Leu Asn 
    1070                 1075                 1080             


Asn Lys  Phe Thr Leu Val Arg  Ala Ser Met Ile Val  Asp Lys Val 
    1085                 1090                 1095             


His Arg  Glu Ile Val Leu Gln  His Leu Ser Lys Tyr  Asp Asn Asn 
    1100                 1105                 1110             


Ser Glu  Val Ala Phe Asp Ser  Lys Thr Ile Lys Ile  Asp Pro Ile 
    1115                 1120                 1125             


Leu Ile  Glu Gly Ile Gly Leu  Lys Glu Val Val Cys  Tyr Glu Asp 
    1130                 1135                 1140             


Val Phe  Lys Ile Arg Lys Lys  Ile Ser Gly Asp Asn  Phe Lys Asn 
    1145                 1150                 1155             


Glu Lys  His Leu Glu Lys Val  Ile Asp Glu Lys Val  Lys Gln Ile 
    1160                 1165                 1170             


Leu Lys  Asp Arg Val Ile Lys  Phe Gln Gly Asn Phe  Lys Glu Ala 
    1175                 1180                 1185             


Phe Ser  Asn Leu Glu Glu Asn  Pro Ile Trp Leu Asn  Lys Ser Lys 
    1190                 1195                 1200             


Gly Ile  Ala Val Lys Ser Val  Ala Ile Lys Gly Val  Asn Asn Ala 
    1205                 1210                 1215             


Glu Ser  Leu His Glu Gly Lys  Asp His Phe Gly Asp  Gln Ile Leu 
    1220                 1225                 1230             


Asp Lys  Asn Lys Lys Gln Ile  Pro Val Asp Phe Val  Ser Thr Gly 
    1235                 1240                 1245             


Asn Asn  His His Val Gly Ile  Tyr Glu Asp Lys Asp  Gly Asn Leu 
    1250                 1255                 1260             


Gln Glu  Arg Val Val Ser Phe  Phe Glu Ala Val Thr  Arg Val Asn 
    1265                 1270                 1275             


Glu Gly  Leu Pro Ile Ile Asp  Lys Thr Tyr Asn Ser  Asp Ile Gly 
    1280                 1285                 1290             


Trp Lys  Phe Lys Phe Thr Met  Lys Gln Asn Glu Met  Phe Val Phe 
    1295                 1300                 1305             


Pro Ser  Asp Asp Phe Asp Pro  Asn Glu Phe Asp Leu  Thr Asp Gly 
    1310                 1315                 1320             


Lys Asn  Ala Ala Lys Ile Ser  Pro Asn Leu Tyr Arg  Val Gln Lys 
    1325                 1330                 1335             


Ile Ala  Thr Lys Asn Tyr Met  Phe Arg His His Leu  Glu Thr Val 
    1340                 1345                 1350             


Val Thr  Asn Asp Leu Glu Phe  Thr Tyr Lys Ser Ile  Arg Ser Thr 
    1355                 1360                 1365             


Gly Trp  Leu Arg Asn Leu Val  Lys Val Arg Ile Asn  His Leu Gly 
    1370                 1375                 1380             


Glu Ile  Val Tyr Val Gly Glu  Tyr 
    1385                 1390     


<210>  1131
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1131

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Lys Glu Asn Asn Glu Glu Leu Ser Ser Ile Ile 
            20                  25                  30          


Arg Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Lys Ser Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Asp Ile Thr Thr Asn Ala Asp Arg Thr Lys Cys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Phe Lys Leu Arg Arg Glu Ala 
65                  70                  75                  80  


Leu Ala Asp Cys Leu Lys Arg His Gly Leu Met Thr Asp Glu Thr Pro 
                85                  90                  95      


Leu Cys Glu Thr Asn Asn Ser Ile Ile Phe Lys Thr Tyr Ser Asn Arg 
            100                 105                 110         


Ala Lys Ala Val Cys Glu Lys Ile Ala Leu Asp Asp Phe Val Arg Val 
        115                 120                 125             


Leu Phe Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Leu 
    130                 135                 140                 


Asn Thr Asp Asp Glu Gly Thr Leu Val Asp Gly Met Asp Val Ala Lys 
145                 150                 155                 160 


Leu Leu Tyr Asn Glu Gln Ile Thr Pro Gly Glu Tyr Leu Leu Arg Leu 
                165                 170                 175     


Phe Lys Lys Gly Lys Lys Thr Arg Pro Asp Phe Tyr Leu Ser Asp Leu 
            180                 185                 190         


Lys Ala Glu Leu Glu Arg Ile Trp Asp Phe Gln Arg Thr Phe Tyr Pro 
        195                 200                 205             


Glu Ile Leu Cys Asn Ala Leu Lys Glu Asp Leu Lys Gly Lys Ser Arg 
    210                 215                 220                 


Thr Gln Ser Ser Ala Ile Phe Tyr Ala Lys Tyr Lys Val Ser Thr Ala 
225                 230                 235                 240 


Lys Ile Ala Gly Lys Asp Lys Arg Leu Gln Glu Leu Thr Trp Arg Thr 
                245                 250                 255     


Asp Ala Leu Arg Lys Gln Leu Thr Ile Asp Glu Val Ala Tyr Val Ile 
            260                 265                 270         


Cys Ser Ile Asn Gly Glu Val Asn Gly Ser Ser Gln Arg Leu Ser Met 
        275                 280                 285             


Ile Ser Asp Arg Ser Lys Leu Leu Tyr Phe Asn His Gln Thr Ile Gly 
    290                 295                 300                 


Gln Tyr Leu Met Gln Arg Leu Glu Glu Asn Pro Asn Ala Gly Leu Lys 
305                 310                 315                 320 


Asn Ile Ile Phe Tyr Arg Gln Asp Tyr Glu Asp Glu Phe Asn Asp Ile 
                325                 330                 335     


Trp Asn Ser Gln Ala Thr Tyr His Lys Glu Leu Thr Pro Glu Leu Lys 
            340                 345                 350         


Lys Glu Val Lys Asp Ile Ile Phe Tyr Gln Arg Gly Leu Lys Ser Cys 
        355                 360                 365             


Lys Trp Leu Ile Asn Tyr Cys Glu Leu Glu His Glu Glu Ile Glu Val 
    370                 375                 380                 


Lys Thr Pro Glu Gly Lys Leu Arg Lys Ile Thr Ile Gly Ser Lys Thr 
385                 390                 395                 400 


Cys Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Val 
                405                 410                 415     


Leu Asn Asn Leu Gln Val Ser Pro Leu Asn Ser Arg Arg Lys Ala Lys 
            420                 425                 430         


Arg Lys Lys Asp Glu Glu Pro Ser Leu Phe Ser Asp Glu Asp Met Asn 
        435                 440                 445             


Thr Ala Cys Arg Tyr Leu Asn Glu Glu Glu Lys Leu Thr Leu Phe Lys 
    450                 455                 460                 


Glu Leu Ser Ile Lys Lys Glu Leu Lys Lys Lys Asp Ala Leu Lys Leu 
465                 470                 475                 480 


Leu Phe Glu Asn Tyr Lys Glu Leu Asp Leu Asn Tyr Lys Val Ile Ser 
                485                 490                 495     


Gly Asn Val Thr Met Ala Asn Leu Tyr Arg Ala Phe Gln Asp Ile Ile 
            500                 505                 510         


Ser Leu Thr Gly Asn Gly Glu Phe Asp Phe Gln Ala Met Pro Ala Glu 
        515                 520                 525             


Glu Ala Glu Lys Thr Val Glu Glu Ile Phe Gly Gly Leu Gly Tyr Lys 
    530                 535                 540                 


Thr Ala Phe Leu His Phe Asp Ala Ser Leu His Asp Lys Glu Phe Glu 
545                 550                 555                 560 


Arg Gln Pro Ala Tyr Arg Leu Trp His Leu Leu Tyr Ser Tyr Ala Gly 
                565                 570                 575     


Asp Gln Ser Lys Thr Gly Ile Glu Ala Leu Val Lys Arg Ile Gln Glu 
            580                 585                 590         


Leu Cys Gly Phe Glu Pro Glu Tyr Ala Arg Ile Leu Ala Asn Ile Ser 
        595                 600                 605             


Phe Ile Pro Asp Tyr Gly Asn Leu Ser Ser Lys Ala Met Arg Lys Ile 
    610                 615                 620                 


Leu Pro Tyr Met Gln Asp Gly Tyr Glu Tyr Ser Glu Ala Cys Ser Lys 
625                 630                 635                 640 


Ala Gly Tyr Arg His Ser Ala Ser Ser Leu Thr Lys Glu Glu Ile Asn 
                645                 650                 655     


Lys Lys Asn Tyr Lys Thr His Leu Asp Glu Ile Lys Arg Asn Ser Leu 
            660                 665                 670         


Arg Asn Pro Ile Val Glu Lys Ile Leu Asn Gln Met Val Asn Val Val 
        675                 680                 685             


Asn Ser Val Ile Asp Thr Tyr Ser Asn Ile Asp Glu Ile Arg Ile Glu 
    690                 695                 700                 


Met Ala Arg Glu Leu Lys Lys Ser Lys Pro Glu Arg Glu Asp Met Ser 
705                 710                 715                 720 


Phe Ala Ile Ser Lys Ala Thr Lys Glu Thr Glu Arg Ile Lys Gly Ile 
                725                 730                 735     


Leu Glu Gln Glu Phe Lys Ile Gln His Val Thr Arg Asn Asp Ile Ile 
            740                 745                 750         


Arg Tyr Arg Leu Tyr Glu Glu Leu Lys Pro Asn Gly Tyr Lys Thr Leu 
        755                 760                 765             


Tyr Ser Asn Thr Tyr Ile Pro Arg Glu Asp Leu Phe Ser Lys Lys Phe 
    770                 775                 780                 


Asp Ile Glu His Ile Ile Pro Lys Ala Arg Leu Phe Asp Asp Ser Tyr 
785                 790                 795                 800 


Ser Asn Lys Thr Leu Glu Leu Arg Asp Ile Asn Ile Glu Lys Gly Asn 
                805                 810                 815     


Gln Thr Ala Tyr Asp Phe Val Leu Ser Lys Tyr Gly Glu Glu Gly Leu 
            820                 825                 830         


Tyr Lys Tyr Glu Gln Arg Val Glu Glu Phe Lys Lys Asn Asn Glu Gln 
        835                 840                 845             


Ala Gly Thr Lys Cys Arg Lys Leu Leu Met Arg Glu Ala Asp Ile Pro 
    850                 855                 860                 


Ser Asp Phe Leu Asn Arg Asp Leu Asn Asp Ser Gln Tyr Ile Ala Lys 
865                 870                 875                 880 


Lys Ala His Glu Leu Leu Arg Glu Ile Val Pro Thr Val Thr Ala Thr 
                885                 890                 895     


Thr Gly Ser Ile Thr Asn Arg Leu Arg Glu Asp Trp Lys Leu Val Asn 
            900                 905                 910         


Val Met Lys Glu Leu Asn Trp Glu Lys Tyr Asp Lys Leu Gly Leu Thr 
        915                 920                 925             


Glu Glu Tyr Lys Asp Lys Asp Gly Asn Thr Val Arg His Ile Lys Asp 
    930                 935                 940                 


Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr 
945                 950                 955                 960 


Ile Ala Phe Thr Lys Pro Ser Phe Ile Gln Tyr Leu Asn Asn Leu Asn 
                965                 970                 975     


Ala Arg Ser Asn Arg Gly Gly Ser Ile Tyr Gly Ile Glu Gln Lys Glu 
            980                 985                 990         


Leu Glu Arg Glu Asn Asn Lys Leu  Val Phe Ile Pro Pro  Ile Asp Pro 
        995                 1000                 1005             


Lys Ile  Phe Arg Lys Gln Ala  Lys Glu His Leu Ser  Asn Ile Leu 
    1010                 1015                 1020             


Ile Ser  Ile Lys Ala Lys Asn  Lys Val Val Thr Lys  Asn Val Asn 
    1025                 1030                 1035             


Lys Thr  Lys Ser Lys Ser Gly  Thr Asn Arg Lys Val  Gln Leu Thr 
    1040                 1045                 1050             


Pro Arg  Gly Gln Leu His Leu  Glu Thr Val Tyr Gly  Lys Ile Arg 
    1055                 1060                 1065             


Thr Tyr  Lys Thr Glu Glu Ile  Lys Val Gly Thr Lys  Leu Thr Glu 
    1070                 1075                 1080             


Glu Leu  Ile Ala Lys Val Ala  Asp Lys Lys Ile Arg  Glu Ala Leu 
    1085                 1090                 1095             


Met Gln  Arg Leu Ser Glu Tyr  Gly Gly Asn Ile Lys  Lys Ala Phe 
    1100                 1105                 1110             


Gln Asn  Ile Ser Lys His Pro  Ile Trp Leu Asn Gly  Ser His Thr 
    1115                 1120                 1125             


Lys His  Val Pro Glu Lys Val  Lys Ile Val Phe Phe  Glu Asp Val 
    1130                 1135                 1140             


Phe Thr  Ile Arg Lys Ala Ile  Thr Pro Asp Ile Lys  Ile Asp Lys 
    1145                 1150                 1155             


Val Ile  Asp Ser Lys Ile Arg  Arg Ile Leu Gln Glu  Arg Leu Asn 
    1160                 1165                 1170             


Glu Phe  Gly Gly Glu Ala Ser  Lys Ala Phe Ser Asn  Leu Asp Glu 
    1175                 1180                 1185             


Asn Pro  Ile Trp Phe Asn Arg  Asp Lys Gly Ile Ala  Ile Lys Ser 
    1190                 1195                 1200             


Val Lys  Ile Arg Gly Ile Ala  Lys Gly Thr Ala Ile  Arg Val Lys 
    1205                 1210                 1215             


Lys Asp  Ile His Gly Lys Thr  Ile Ile Gly Asn Asn  Gly Tyr Pro 
    1220                 1225                 1230             


Val Ala  Asn Asp Tyr Val Asn  Thr Gly Asn Asn His  His Val Ala 
    1235                 1240                 1245             


Ile Phe  Arg Asp Lys Asp Gly  Asn Leu Gln Glu His  Ile Val Ser 
    1250                 1255                 1260             


Phe Tyr  Glu Ala Thr Ala Arg  Ala Ile Gln Asn Leu  Pro Ile Val 
    1265                 1270                 1275             


Asp Arg  Asn Tyr Asn Ser Glu  Lys Asp Trp Lys Phe  Leu Phe Ser 
    1280                 1285                 1290             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Pro  Asn Thr Gly 
    1295                 1300                 1305             


Phe Asn  Pro Ser Glu Ile Ser  Leu Lys Asp Glu Lys  Asn Tyr Ala 
    1310                 1315                 1320             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Leu  Ser His Tyr 
    1325                 1330                 1335             


Tyr Tyr  Val Phe Arg His His  Leu Glu Thr Asn Val  Asp Thr Asn 
    1340                 1345                 1350             


Leu Gln  Leu Lys Asp Ile Thr  Trp Lys Arg Ile Thr  Ala Val Asn 
    1355                 1360                 1365             


Asn Leu  Thr Gly Ala Val Lys  Val Arg Ile Asp His  Leu Gly Arg 
    1370                 1375                 1380             


Ile Val  Asp Val Gly Glu Tyr  Lys 
    1385                 1390     


<210>  1132
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1132

Met Lys Asn Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser Val Gly 
1               5                   10                  15      


Phe Ala Ala Arg Asp Asp Asp Asn Asn Leu Ile Arg Val Lys Gly Lys 
            20                  25                  30          


Asn Val Ile Gly Ala Arg Leu Phe Ser Glu Gly Gln Thr Ala Gln Glu 
        35                  40                  45              


Arg Arg Met Ala Arg Thr Ser Arg Arg Arg Tyr Asn Arg Arg Lys Trp 
    50                  55                  60                  


Arg Leu Arg Leu Leu Asp Glu Ile Phe His Asp Glu Ile Ile His Lys 
65                  70                  75                  80  


Asp Ala Glu Phe Phe Asn Arg Leu Lys Gln Ser Phe Leu Ser Pro Lys 
                85                  90                  95      


Asp Glu Arg Lys Gln Tyr Phe Gly Ser Leu Leu Phe Pro Glu Thr Gly 
            100                 105                 110         


Asp Ser Glu Tyr Tyr His Asn Gly Asn Gln Thr Ile Tyr His Leu Arg 
        115                 120                 125             


His Arg Leu Ile Thr Gln Asp Glu Lys Ala Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Leu Arg His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Asp Asp 
145                 150                 155                 160 


Thr Pro Val Ser Ser Phe Glu Ala Ser Asp Leu Lys Leu Ala Ser Phe 
                165                 170                 175     


Val Pro Thr Ile Asn Asp Leu Phe Glu Thr Ile Asp Val Asn Tyr Glu 
            180                 185                 190         


Phe Ser Met Asp Asn Ile Asn Glu Ile Glu Asp Ile Leu Leu Asn Asn 
        195                 200                 205             


Lys Leu Arg Asn Ser Asp Lys Ser Lys Gln Leu Thr Asp Leu Leu Thr 
    210                 215                 220                 


Glu Pro Ile Val Asn Ile Asp Asp Lys Ser Leu Glu Lys Gln Phe Asn 
225                 230                 235                 240 


Lys Glu Arg Thr Asp Ile Ser Lys Glu Ile Ala Lys Ser Val Val Gly 
                245                 250                 255     


Ser Lys Met Asn Val Asn Lys Leu Leu Ser Phe Glu Ser Ser Glu Gly 
            260                 265                 270         


Ser Lys Ile Ala Phe Ser Phe Ser Asp Ala Ser Ser Glu Asp Lys Met 
        275                 280                 285             


Met Glu Leu Ser Asp Ile Leu Asp Asp Ser Arg Met Asn Ile Leu Asn 
    290                 295                 300                 


Ile Leu Lys Val Ile Tyr Ser Arg Val Arg Leu Asn Gln Ile Ile Pro 
305                 310                 315                 320 


Ser Gly Met Gly Leu Ser Glu Ser Met Val Ala Ala Tyr Asp Ser His 
                325                 330                 335     


Lys Gln Gln Leu Lys Asp Leu Lys Thr Leu Leu Asn Val Leu Pro Phe 
            340                 345                 350         


Asn Glu Arg Tyr Ala Val Lys Ile Ala Tyr Ala Ala Tyr Ile Gly Asn 
        355                 360                 365             


Ile Asp Lys Gln Lys Phe Thr Lys Ser Glu Ile Lys Ala Ile Asp Lys 
    370                 375                 380                 


Leu Lys Glu Lys Ser Ala Ile Met Asn Val Leu Asp Phe Lys Ser Gly 
385                 390                 395                 400 


His Ile Thr Asn Glu Glu Leu Ser Asn Val Ile Lys Ala Val Ile Val 
                405                 410                 415     


Asp Lys Tyr Leu Asn Asp Glu Asp Phe Glu Tyr His Gly Ile Val Glu 
            420                 425                 430         


Lys Ile Asn Gln Asp Ile Ser Asn Gly Ser Tyr Leu Leu Lys Gln Arg 
        435                 440                 445             


Asn Asn Gln Asn Gly Asn Ile Pro His Gln Leu His Gln Met Glu Leu 
    450                 455                 460                 


Asp Gln Ile Ile Glu Asn Gln Ser Lys Tyr Tyr His Phe Leu Gly Glu 
465                 470                 475                 480 


Leu Asn Pro Asn Ser Asn Arg Asn His Ile Ala Lys Tyr Lys Ile Ser 
                485                 490                 495     


Glu Leu Val Ala Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr 
            500                 505                 510         


Pro Glu Glu Gln Glu Lys Thr Ser Gly Ala Ser Phe Ala Trp Met Lys 
        515                 520                 525             


Arg Lys Lys Asp Gly Ala Ile Thr Pro Trp Asn Phe Glu Glu Lys Val 
    530                 535                 540                 


Asp Lys Glu Glu Thr Ala Ser Arg Phe Ile Lys Arg Leu Thr Val Lys 
545                 550                 555                 560 


Asp Thr Tyr Leu Leu Asn Glu Asp Val Leu Pro Asp Asn Ser Leu Leu 
                565                 570                 575     


Tyr Glu Lys Phe Lys Val Leu Asn Glu Leu Asn Val Val Lys Ala Asn 
            580                 585                 590         


Asn Lys Arg Leu Thr Val Glu Glu Lys Gln Gly Ala Phe Asp Asn Leu 
        595                 600                 605             


Phe Lys Lys Met Lys Thr Val Thr Ala Lys Arg Leu Lys Asn Tyr Leu 
    610                 615                 620                 


Val Ser Asn His Asn His Ile Asn Ser Val Glu Ile Thr Gly Leu Ser 
625                 630                 635                 640 


Asp Gly Asp Lys Phe Asn Asn Ser Leu Ser Thr Tyr Ile Asp Phe Lys 
                645                 650                 655     


Lys Ile Phe Gly Asp Lys Ile Asp Asn Pro Gly Leu Leu Asp Asp Phe 
            660                 665                 670         


Glu Asp Ile Ile Glu Trp Ser Thr Val Phe Glu Asp Arg Lys Ile Phe 
        675                 680                 685             


Glu Glu Lys Val Arg Lys Asp Ile Ser Trp Ile Ser Glu Ser Glu Ile 
    690                 695                 700                 


Lys Ser Val Val Ser Lys Arg Tyr Ser Gly Trp Gly Arg Leu Ser Lys 
705                 710                 715                 720 


Lys Leu Leu Asn Cys Ile Thr Asn Asn Glu Gly Arg Asn Ile Ile Asp 
                725                 730                 735     


Glu Leu Trp Glu Glu Ser Glu Thr Phe Met Glu Ala Val Ser Lys Pro 
            740                 745                 750         


Glu Val Lys Glu Gln Ile Asp Gly Ile Asn Ser Asp Phe Ile Lys Asn 
        755                 760                 765             


Thr Asp Ile Glu Ser Val Leu Ala Asp Ala Tyr Thr Ser Pro Gln Asn 
    770                 775                 780                 


Lys Lys Ala Ile Arg Glu Thr His Lys Leu Val Lys Asp Ile Gln Lys 
785                 790                 795                 800 


Ala Met Gly Gly Gln Ala Pro Ala Ser Ile Ser Ile Glu Phe Thr Arg 
                805                 810                 815     


Asn Pro Gln Asp Lys Gly Gln Leu Thr Arg Ser Arg His Ser Glu Val 
            820                 825                 830         


Ser Arg Lys Tyr Lys Glu Ile Ser Lys Glu Ile Ser Lys Thr Leu Asn 
        835                 840                 845             


Asp Glu Leu Lys Ser Lys Lys Asn Ser Met Asp Val Asp Lys Tyr Tyr 
    850                 855                 860                 


Leu Tyr Phe Met Gln Met Gly Ile Asp Leu Tyr Asp Gly Ser Ser Ile 
865                 870                 875                 880 


Asp Ile Asp Asp Leu Asn Asn Tyr Gln Ile Asp His Ile Ile Pro Gln 
                885                 890                 895     


Ser Phe Tyr Lys Asp Asp Ser Phe Asp Asn Arg Val Leu Thr His Tyr 
            900                 905                 910         


Ser Asn Asn Gln Lys Lys Gly Asn Asn Thr Pro Leu Asn Gly Leu Asn 
        915                 920                 925             


Ile Asn Asp Lys Thr Arg Ala Ile Trp Asn His Leu Arg Asp Leu Gly 
    930                 935                 940                 


Leu Ile Thr Lys Lys Lys Tyr Asn Gly Leu Thr Leu Lys Gly Met Asp 
945                 950                 955                 960 


Gln Leu Ser Lys Tyr Thr Lys His Gly Phe Ile Arg Arg Gln Leu Val 
                965                 970                 975     


Glu Thr Ser Gln Val Ile Lys Leu Val Ala Asn Ile Leu Ser Asp Glu 
            980                 985                 990         


Phe Ser Thr Asp Asp Thr Lys Ile  Ile Glu Val Lys Ala  Lys Met Asn 
        995                 1000                 1005             


Ser Gln  Ile Arg Gln Val Phe  Asn Leu Tyr Lys Val  Arg Glu Leu 
    1010                 1015                 1020             


Asn Asp  Tyr His His Ala Val  Asp Ala Tyr Leu Thr  Thr Phe Ile 
    1025                 1030                 1035             


Gly Gly  Tyr Leu Tyr Asn Arg  Tyr Pro Asn Leu Arg  Asn Met Phe 
    1040                 1045                 1050             


Val Tyr  Gly Asp Phe Lys Lys  Phe Ser Ala Asp Val  Asp Asn Leu 
    1055                 1060                 1065             


Lys Thr  Phe Asn Phe Leu His  Glu Ile Thr Asn Pro  Ser Asp Lys 
    1070                 1075                 1080             


Phe Glu  Asp Arg Ile Phe Asp  Glu Lys Ser Gly Glu  Leu Ile Leu 
    1085                 1090                 1095             


Asp Arg  Lys His Ala Ile Glu  Thr Ile Lys Lys Ile  Tyr Gln Tyr 
    1100                 1105                 1110             


Lys Tyr  Met Leu Ile Thr Arg  Glu Val Ser Thr Lys  Asn Gly Gln 
    1115                 1120                 1125             


Leu Ser  Asp Gln Thr Val Tyr  Ser Ala Lys Arg Ile  Asn Asn Val 
    1130                 1135                 1140             


Lys Ala  Pro Ile Pro Val Lys  Lys Asp Phe Asn Pro  Glu Leu Tyr 
    1145                 1150                 1155             


Gly Phe  Arg Thr Gly Asn Val  Asp His His Met Val  Ile Val Lys 
    1160                 1165                 1170             


Tyr Pro  Lys Lys Asp Ser Tyr  Asp Tyr Lys Val Val  Gly Ile Pro 
    1175                 1180                 1185             


Leu Arg  Phe Val Gln Lys Leu  Lys Glu Thr Lys Asp  Asn Lys Asp 
    1190                 1195                 1200             


Tyr Glu  Asn Leu Leu Asn Gln  Ile Val Gln Met Lys  Leu Asn Arg 
    1205                 1210                 1215             


Lys Lys  Gln Asp Phe Ile Val  Val Val Asp Asn Val  Met Tyr Gly 
    1220                 1225                 1230             


Gln Leu  Ile Gln Asp Gly Gly  Gln Lys Phe Thr Leu  Gly Ser Ser 
    1235                 1240                 1245             


Thr Tyr  Arg Tyr Asn Ala Lys  Gln Leu Val Ile Asp  Asn Gly Tyr 
    1250                 1255                 1260             


Leu Lys  Leu Ile Ala Asp Glu  Arg Tyr Lys Asn Ser  Leu Thr Asp 
    1265                 1270                 1275             


Glu Glu  Lys Ser Asn Gln Tyr  Ile Thr Leu Tyr Asn  Glu Ile Leu 
    1280                 1285                 1290             


Lys Val  Val Asn Gly Arg Phe  Asp Val Phe Asp Val  Asn Ser Phe 
    1295                 1300                 1305             


Arg Lys  Lys Leu Asn Asn Gly  Val Asp Lys Phe Ile  Lys Leu Asn 
    1310                 1315                 1320             


Thr Val  Asp Lys Arg Ile Ile  Ile Met Lys Ile Leu  Ser Gly Leu 
    1325                 1330                 1335             


His Cys  Gly Pro Ser Ile Val  Pro Leu Lys Glu Leu  Gly Leu Thr 
    1340                 1345                 1350             


Thr Pro  Leu Gly Gln Leu Gln  Ser Thr Ser Gly Ile  Ser Leu Ser 
    1355                 1360                 1365             


Glu Asn  Thr Ile Ile Ile Tyr  Gln Ser Pro Ser Gly  Leu Phe Glu 
    1370                 1375                 1380             


Arg Lys  Val Arg Leu Gly Asp  Leu 
    1385                 1390     


<210>  1133
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1133

Met Lys Asn Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser Val Gly 
1               5                   10                  15      


Phe Ala Ala Arg Asp Asp Asp Asn Asn Leu Ile Arg Val Lys Gly Lys 
            20                  25                  30          


Asn Val Ile Gly Ala Arg Leu Phe Ser Glu Gly Lys Thr Ala Gln Glu 
        35                  40                  45              


Arg Arg Ile Ser Arg Thr Ser Arg Arg Arg Tyr Asn Arg Arg Lys Trp 
    50                  55                  60                  


Arg Leu Arg Leu Leu Asp Glu Ile Phe His Asp Ala Ile Val His Lys 
65                  70                  75                  80  


Asp Ala Glu Phe Phe Asn Arg Leu Lys Gln Ser Ser Leu Ser Pro Lys 
                85                  90                  95      


Asp Glu Arg Lys Gln Tyr Phe Gly Ser Leu Leu Phe Pro Glu Val Gly 
            100                 105                 110         


Asp Gly Asp Tyr Phe Arg Asp Gly Asn Gln Thr Ile Tyr His Leu Arg 
        115                 120                 125             


His Arg Leu Met Thr Gln Asp Glu Lys Ala Asp Ile Arg Glu Val Tyr 
    130                 135                 140                 


Leu Ala Leu Arg His Ile Ile Lys Tyr Arg Gly Asn Phe Leu Asp Asp 
145                 150                 155                 160 


Thr Pro Val Ser Ser Phe Glu Ala Ser Asp Leu Lys Leu Ala Ser Phe 
                165                 170                 175     


Val Pro Thr Leu Asn Asp Leu Phe Glu Thr Ile Asn Val Asn Tyr Glu 
            180                 185                 190         


Leu Ser Ile Asp Asn Val Asn Gln Ile Glu Asn Ile Leu Leu Asn Asn 
        195                 200                 205             


Lys Leu Arg Asn Ser Asp Lys Ser Lys Gln Leu Thr Asp Leu Ile Ala 
    210                 215                 220                 


Glu Pro Val Thr Asn Ile Asp Asp Lys Ser Leu Lys Lys Gln Phe Asp 
225                 230                 235                 240 


Lys Glu Arg Lys Asp Ile Ser Lys Glu Ile Ala Lys Ala Val Val Gly 
                245                 250                 255     


Ser Lys Met Asn Val Asn Lys Leu Leu Ser Phe Glu Ser Ser Asp Gly 
            260                 265                 270         


Ser Lys Ile Ser Phe Ser Phe Ser Asp Ala Ser Ser Glu Asp Lys Met 
        275                 280                 285             


Leu Glu Leu Ser Asp Ile Leu Asp Asp Ser Arg Met Asn Ile Leu Asn 
    290                 295                 300                 


Val Leu Lys Val Ile Tyr Ser Arg Val Arg Leu Asn Gln Ile Ile Pro 
305                 310                 315                 320 


Ser Gly Met Gly Leu Ser Glu Ser Met Ile Ala Ala Tyr Asp Leu His 
                325                 330                 335     


Lys Gln Gln Leu Lys Asp Leu Lys Thr Leu Leu Asp Val Val Pro Phe 
            340                 345                 350         


Asn Glu Lys Tyr Ala Val Lys Ile Ala Tyr Ala Ala Tyr Ile Gly Asn 
        355                 360                 365             


Ile Asp Lys Gln Arg Phe Thr Lys Ala Glu Val Lys Thr Ile Asp Lys 
    370                 375                 380                 


Leu Arg Asp Lys Lys Ser Ile Thr Ser Val Leu Asp Phe Lys Ser Gly 
385                 390                 395                 400 


His Ile Thr Asn Glu Glu Leu Asn Asn Val Ile Lys Glu Val Ile Val 
                405                 410                 415     


Asp Lys Tyr Leu Asn Asp Glu Thr Phe Glu Tyr His Asp Ile Val Glu 
            420                 425                 430         


Lys Ile Asn Gln Asp Ile Ser Asn Gly Asn Tyr Leu Leu Lys Gln Arg 
        435                 440                 445             


Asn Asn Gln Asn Gly Asn Ile Pro His Gln Leu His Gln Met Glu Leu 
    450                 455                 460                 


Asp Gln Ile Ile Glu Lys Gln Ser Lys Tyr Tyr Pro Phe Leu Gly Lys 
465                 470                 475                 480 


Leu Asn Pro Asn Ser Lys Arg Ser His Ile Ala Lys Tyr Lys Ile Ser 
                485                 490                 495     


Glu Leu Val Ala Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr 
            500                 505                 510         


Pro Glu Glu Gln Glu Lys Thr Ser Gly Ala Ser Phe Ala Trp Met Lys 
        515                 520                 525             


Arg Lys Glu Asp Gly Ala Ile Thr Pro Trp Asn Phe Glu Lys Met Val 
    530                 535                 540                 


Asp Lys Glu Ser Thr Ala Ser Arg Phe Ile Lys Arg Leu Thr Val Lys 
545                 550                 555                 560 


Asp Thr Tyr Leu Leu Asn Glu Asp Val Leu Pro Asp Asn Ser Leu Leu 
                565                 570                 575     


Tyr Glu Lys Phe Lys Val Leu Asn Glu Leu Asn Val Val Lys Ala Asn 
            580                 585                 590         


Asn Lys Arg Leu Thr Val Glu Glu Lys Gln Gly Ala Phe Asp Asn Leu 
        595                 600                 605             


Phe Lys Gln Met Lys Thr Val Thr Ala Lys Arg Leu Lys Asn Tyr Leu 
    610                 615                 620                 


Val Ser Asn His Asn His Ile Asn Ser Val Lys Ile Thr Gly Leu Ser 
625                 630                 635                 640 


Asp Gly Glu Lys Phe Asn Asn Ser Leu Ser Val Tyr Ile Asp Phe Lys 
                645                 650                 655     


Lys Ile Phe Gly Asp Lys Ile Asp Asp Ser Lys Leu Phe Asp Asp Phe 
            660                 665                 670         


Glu Asn Ile Ile Glu Trp Ser Thr Val Phe Glu Asp Arg Asp Ile Phe 
        675                 680                 685             


Glu Glu Lys Val Arg Lys Asp Ile Ser Trp Ile Ser Glu Ser Glu Ile 
    690                 695                 700                 


Asn Ala Val Val Ser Lys Arg Tyr Ser Gly Trp Gly Arg Leu Ser Lys 
705                 710                 715                 720 


Lys Leu Leu Asn Gly Ile Thr Asn Asp Glu Gly Arg Asn Ile Ile Asp 
                725                 730                 735     


Glu Leu Trp Glu Glu Ser Glu Thr Phe Met Glu Ala Val Ser Lys Pro 
            740                 745                 750         


Glu Val Lys Glu Lys Ile Asp Glu Ile Asn Ser Asn Phe Ile Lys Asn 
        755                 760                 765             


Thr Asp Ile Glu Ser Val Leu Ala Asp Ala Tyr Thr Ser Pro Gln Asn 
    770                 775                 780                 


Lys Lys Ala Ile Arg Glu Thr Tyr Lys Leu Val Lys Asp Ile Gln Lys 
785                 790                 795                 800 


Ala Met Gly Gly Gln Ala Pro Ser Ser Ile Ser Ile Glu Phe Thr Arg 
                805                 810                 815     


Asn Pro Gln Asp Lys Gly Glu Leu Thr Arg Ser Arg His Ser Glu Ile 
            820                 825                 830         


Ala Lys Lys Tyr Lys Glu Leu Ser Lys Glu Val Ser Lys Lys Leu Asn 
        835                 840                 845             


Asp Glu Leu Lys Ser Lys Lys Asn Ser Met Asp Val Asp Lys Tyr Tyr 
    850                 855                 860                 


Leu Tyr Phe Met Gln Met Gly Ile Asp Leu Tyr Asp Gly Thr Ser Ile 
865                 870                 875                 880 


Asp Ile Asp Asp Leu Asn Asn Tyr Gln Ile Asp His Ile Val Pro Gln 
                885                 890                 895     


Ser Phe Tyr Lys Asp Asp Ser Phe Asp Asn Arg Val Leu Thr His Tyr 
            900                 905                 910         


Ser Asn Asn Gln Lys Lys Gly Asn Asn Thr Pro Leu Asn Gly Leu Asn 
        915                 920                 925             


Ile Asp Ser Asn Thr Arg Glu Val Trp Asn His Leu Ile Gly Leu Gly 
    930                 935                 940                 


Leu Ile Thr Lys Arg Lys Tyr Asn Asn Leu Thr Leu Lys Gly Met Asp 
945                 950                 955                 960 


Gln Leu Ser Lys Tyr Thr Lys His Gly Phe Ile Arg Arg Gln Leu Val 
                965                 970                 975     


Glu Thr Ser Gln Val Ile Lys Leu Val Ala Asn Ile Leu Ser Asp Glu 
            980                 985                 990         


Tyr Ser Lys Asp Asp Thr Lys Ile  Ile Glu Val Lys Ala  Lys Leu Asn 
        995                 1000                 1005             


Ser Gln  Ile Arg Gln Val Phe  Asn Leu Tyr Lys Ile  Arg Glu Leu 
    1010                 1015                 1020             


Asn Asp  Tyr His His Ala Leu  Asp Ala Tyr Leu Thr  Thr Phe Ile 
    1025                 1030                 1035             


Gly Gly  Tyr Leu Tyr Asn Arg  Tyr Pro Asn Leu Arg  Asn Met Phe 
    1040                 1045                 1050             


Val Tyr  Gly Asp Phe Lys Lys  Phe Ser Ala Lys Ile  Asp Asn Leu 
    1055                 1060                 1065             


Glu Thr  Phe Asn Phe Leu His  Asp Ile Thr Asn Pro  Ser Asp Lys 
    1070                 1075                 1080             


Phe Glu  Asp Arg Ile Ile Asp  Glu Lys Ser Gly Glu  Val Ile Leu 
    1085                 1090                 1095             


Asp Arg  Arg His Ala Ile Glu  Thr Ile Lys Lys Ile  Tyr Gln Tyr 
    1100                 1105                 1110             


Asn Tyr  Met Leu Ile Thr Arg  Glu Val Ser Thr Lys  Asn Gly Gln 
    1115                 1120                 1125             


Leu Ser  Asp Gln Thr Val Tyr  Ser Ala Arg Arg Ile  Asn Lys Val 
    1130                 1135                 1140             


Asn Ser  Pro Ile Pro Val Lys  Lys Asp Ser Asn Pro  Glu Leu Tyr 
    1145                 1150                 1155             


Gly Phe  Arg Thr Gly Asn Val  Asp His His Met Val  Ile Val Lys 
    1160                 1165                 1170             


Tyr Leu  Asn Lys Asp Lys Tyr  Asp Tyr Lys Ile Ile  Gly Ile Pro 
    1175                 1180                 1185             


Leu Arg  Phe Val Gln Val Leu  Lys Asn Ser Asn Asp  Asn Thr Glu 
    1190                 1195                 1200             


Tyr Lys  Asn Ile Leu Asn Lys  Ile Val Gln Ser Lys  Leu Lys Gly 
    1205                 1210                 1215             


Lys Ser  Thr Asp Phe Arg Val  Ile Leu Asp Asn Val  Met Tyr Gly 
    1220                 1225                 1230             


Gln Leu  Ile Gln Asp Gly Gly  Gln Lys Phe Thr Leu  Gly Ser Ser 
    1235                 1240                 1245             


Thr Tyr  Lys Tyr Asn Ala Lys  Gln Leu Val Ile Asn  Met Asp Asp 
    1250                 1255                 1260             


Ile Arg  Ile Leu Val Asp Asn  Arg Tyr Phe Asn Ser  Leu Ser Asp 
    1265                 1270                 1275             


Asp Asp  Ala Ser Gly Glu Phe  Ile Lys Ile Tyr Asn  Glu Ile Leu 
    1280                 1285                 1290             


Lys Val  Ile Asn Ala Arg Phe  Asp Leu Phe Asp Ile  Asn Lys Phe 
    1295                 1300                 1305             


Arg Glu  Lys Leu Asn Asn Gly  Lys Glu Tyr Phe Glu  Asn Leu Ser 
    1310                 1315                 1320             


Ile Asp  Glu Lys Asn Gln Val  Leu Leu Lys Met Leu  Ser Gly Leu 
    1325                 1330                 1335             


His Cys  Gly Ser Ala Asn Val  Pro Ile Lys Glu Leu  Gly Leu Thr 
    1340                 1345                 1350             


Thr Pro  Leu Gly Lys Leu Gln  Ile Pro Ser Gly Ile  Val Leu Ser 
    1355                 1360                 1365             


Glu Asp  Ala Ile Val Ile Tyr  Gln Ser Pro Ser Gly  Leu Phe Glu 
    1370                 1375                 1380             


Arg Lys  Ile Arg Leu Lys Asp  Ile 
    1385                 1390     


<210>  1134
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1134

Met Asn Leu Arg Asn Tyr Pro Gly Glu Tyr Ser Ile Gly Leu Asp Met 
1               5                   10                  15      


Gly Thr Gly Ser Val Gly Trp Ser Val Val Asp Glu Lys Gly Asn Leu 
            20                  25                  30          


Leu His Phe Lys Lys Lys Pro Thr Trp Gly Ser Arg Leu Phe Asp Ser 
        35                  40                  45              


Val Met Pro Ala Ser Glu Ala Arg Val His Arg Gly Gln Arg Arg Arg 
    50                  55                  60                  


Tyr Glu Arg Arg Arg Trp Arg Leu Asp Leu Leu Gln Gly Leu Phe Lys 
65                  70                  75                  80  


Asn Glu Met Asn Lys Val Asp Pro Glu Phe Phe Met Arg Leu Asn Gln 
                85                  90                  95      


Ser Arg Thr Ile Glu Gly Asp Pro Ile Phe Thr Lys Ser Phe Thr Lys 
            100                 105                 110         


Ala Asp Tyr Tyr Asp Arg Phe Pro Thr Ile Tyr His Leu Arg Ala Tyr 
        115                 120                 125             


Leu Met Glu Ser Asn Glu Pro Ala Asp Leu Arg Leu Ile Tyr Leu Ala 
    130                 135                 140                 


Met His Asn Ile Ile Lys His Arg Gly Asn Phe Leu Arg Glu Glu Glu 
145                 150                 155                 160 


Lys Ser Leu Ser Ala Lys His Ala Asp Ser Lys Lys Ala Val Thr Gln 
                165                 170                 175     


Phe Val Asp Ala Leu Lys Glu Trp Cys Phe Ser Arg Gly Tyr Glu Phe 
            180                 185                 190         


Gly Lys Asp Pro Ala Lys Ser Ile Glu Lys Cys Leu Ser Asp Ser Lys 
        195                 200                 205             


Ser Ser Arg Ser Gln Leu Ala Ser Glu Ile Ala Lys Ala Leu Ala Val 
    210                 215                 220                 


Ser Thr Asp Ala Ala Ala Ala Asp Lys Lys Leu Ala Asp Asn Leu Ala 
225                 230                 235                 240 


Lys Ala Met Val Gly Leu Gln Cys Glu Phe Lys Tyr Val Phe Gly Glu 
                245                 250                 255     


Phe Asp Cys Glu Ser Thr Lys Leu Ala Leu Ser Asp Asp Glu Lys Leu 
            260                 265                 270         


Glu Leu Leu Arg Ala Ala Cys Pro Asp Asp Ser Val Glu Leu Leu Asp 
        275                 280                 285             


Ala Leu Cys Ala Val Tyr Ser Ala Tyr Val Leu Gln Gly Leu Leu Ser 
    290                 295                 300                 


Tyr Ala Pro Gly Gln Thr Ile Ser His Asn Met Ile Ala Lys Tyr Asp 
305                 310                 315                 320 


Leu Tyr Lys Thr Asp Leu Lys Thr Leu Arg Ala Leu Val Arg Glu Tyr 
                325                 330                 335     


Ala Pro Gly Ala Tyr Asp Glu Phe Phe Arg Gly Ala Thr Tyr His Asp 
            340                 345                 350         


Ala Asn Pro Asp Asp Ser Ser Lys Asp Tyr Ser Tyr Ala Asp Ala Glu 
        355                 360                 365             


Gly Tyr Thr Ala Tyr Asn Leu His Lys Leu Ser Tyr Asp Asp Phe Ala 
    370                 375                 380                 


Lys Ala Val Lys Lys Leu Leu Gly Gly Thr Asp Ala Val Thr Asp Glu 
385                 390                 395                 400 


Arg Tyr Leu Asn Met Met Glu Arg Phe Glu Lys Gln Gln Phe Leu Arg 
                405                 410                 415     


Arg Leu Lys Thr Ser Asp Asn Gly Ala Ile Tyr Tyr Gln Leu His Leu 
            420                 425                 430         


Glu Glu Leu Thr Ala Ile Ile Lys Asn Gln Gly Arg Phe Tyr Ser Phe 
        435                 440                 445             


Leu Lys Glu Tyr Glu Gly Lys Phe Glu Ser Leu Val Ser Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Thr Ser Val Asn Ala Ala Gln Asp Ser 
465                 470                 475                 480 


His Asn His Asn Arg Phe Gln Trp Ser Glu Arg Lys Pro Gly Met Glu 
                485                 490                 495     


Arg Glu Val Ile Thr Pro Trp Asn Trp Asp Thr Ile Ile Asp Lys Asn 
            500                 505                 510         


Lys Ser Ala Glu Asn Phe Ile Met Arg Met Thr Gly Asp Cys Thr Tyr 
        515                 520                 525             


Leu Ala Gly Glu Lys Val Leu Pro Lys Gln Ser Leu Leu Tyr Glu Glu 
    530                 535                 540                 


Phe Cys Val Leu Asn Glu Leu Asn Gly Val Arg Trp Ser Asp Asp Gly 
545                 550                 555                 560 


Asp Asp Trp Gln Arg Phe Asp Ala Ala Gln Arg Glu Gly Ile Val Arg 
                565                 570                 575     


Glu Leu Phe His Lys Tyr Arg Arg Val Thr Tyr Lys Met Leu Glu Asp 
            580                 585                 590         


Trp Leu Val Arg Glu Gly Asp Ala Asn His Pro Ile Val Lys Gly Gly 
        595                 600                 605             


Gln Gly Glu Thr Gly Leu Glu Ser Lys Met Gly Ser Tyr Leu Phe Phe 
    610                 615                 620                 


Ala Lys Gly Ile Phe Gly Leu Gly Glu Leu Ser Pro Ala Glu Tyr Pro 
625                 630                 635                 640 


Ala Ile Glu Lys Met Ile Lys Trp Asn Thr Leu Phe Glu Asp Arg Ser 
                645                 650                 655     


Ile Leu Lys Glu Lys Leu Glu Asp Glu Phe Gly Glu Arg Gly Asn Gly 
            660                 665                 670         


Met Leu Asp Ala Ser Gln Ile Lys Ala Ile Cys Lys Lys Arg Phe Thr 
        675                 680                 685             


Gly Trp Gly Arg Leu Ser Glu Gln Phe Leu Thr Gly Leu Lys Val Glu 
    690                 695                 700                 


Ser Pro Ala Gly Arg Ala Val Ser Ile Met Asp Val Leu Arg Glu Gly 
705                 710                 715                 720 


Asn Pro Cys Ser Glu Arg Arg Gln Gly Glu Thr Val Val Met Met Glu 
                725                 730                 735     


Ala Leu Arg Asp Glu Gly Leu Gly Phe Gln Lys Lys Ile Asp Glu His 
            740                 745                 750         


Asn Arg Asn Tyr Tyr Gly Gln His Gly Lys Asp Leu Gly Val Asn Glu 
        755                 760                 765             


Leu Pro Gly Ser Pro Ala Ile Arg Arg Ser Leu Asn Gln Ala Ile Arg 
    770                 775                 780                 


Ile Val Glu Glu Ile Ala Ser Ile Ala Gly His Ala Pro Ala Asn Ile 
785                 790                 795                 800 


Phe Ile Glu Val Thr Arg Gly Glu Asp Ala Gly Lys Lys Gly Lys Arg 
                805                 810                 815     


Thr Val Lys Arg Trp Asp Lys Ile Gly Glu Ala Leu Lys Lys Leu Lys 
            820                 825                 830         


Glu Glu Gly Val Ala Val Glu Val Met Asp Asp Phe Lys Glu Gln Lys 
        835                 840                 845             


Ala Ile Asn Ala Asn Leu Asp Glu Arg Leu Ser Leu Tyr Leu Met Gln 
    850                 855                 860                 


Asn Gly Lys Cys Leu Tyr Ser Gly Lys Ala Ile Asn Leu Glu Lys Leu 
865                 870                 875                 880 


Leu Ala Gly Ser Gly Glu Tyr Glu Val Asp His Ile Ile Pro Arg Ala 
                885                 890                 895     


Tyr Ile Lys Asp Asp Ser Ile Glu Asn Lys Ala Leu Val Leu Arg Glu 
            900                 905                 910         


Glu Asn Gln His Lys Thr Asp Gln Leu Leu Ile Asp Lys Ser Ile Arg 
        915                 920                 925             


Trp Lys Met Arg Glu Thr Trp Ser Gln Leu His Asp Ala Gly Leu Ile 
    930                 935                 940                 


Gly Asp Lys Lys Tyr His Asn Leu Leu Arg Ser Asn Ile Asn Glu Asn 
945                 950                 955                 960 


Gln Leu Arg Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Ser Gln Met 
                965                 970                 975     


Val Lys Leu Ala Gln Ala Leu Leu Glu Ala Arg Phe Ala Asn Glu Glu 
            980                 985                 990         


Thr Arg Ile Val Pro Val Lys Ala  Ser Met Ser His Asn  Leu Arg Glu 
        995                 1000                 1005             


Ala Ala  Asp Leu Ala Lys Cys  Arg Glu Ala Asn Asp  Phe His His 
    1010                 1015                 1020             


Ala His  Asp Ala Phe Leu Ala  Ser Arg Ile Gly Leu  Phe Val Gln 
    1025                 1030                 1035             


Met Arg  His Pro Gly Ile Tyr  Glu Asn Pro Ile Gly  Tyr Ser His 
    1040                 1045                 1050             


Val Ile  Lys Lys Tyr Val Gln  Glu Gln Ser Glu Val  Phe Lys Lys 
    1055                 1060                 1065             


Thr His  Arg Met Pro Gly Ser  Ala Gly Phe Val Ile  Asn Ser Phe 
    1070                 1075                 1080             


Met Ser  Ser Gly Phe Asp Lys  Asp Thr Gly Glu Val  Phe Lys Asp 
    1085                 1090                 1095             


Ser Trp  Asp Ala Glu Ala Glu  Val Asp Gly Ile Arg  Lys Ala Leu 
    1100                 1105                 1110             


Asn Tyr  Arg Gln Cys Tyr Ile  Ser Arg Met Pro Gln  Gln Asp Thr 
    1115                 1120                 1125             


Gly Ala  Phe Trp Asn Ala Thr  Val Tyr Ser Pro Arg  Asp Pro Lys 
    1130                 1135                 1140             


Met Gly  Pro Lys Leu Ala Leu  Ser Thr Lys Gln Gly  Leu Asp Pro 
    1145                 1150                 1155             


Lys Leu  Tyr Gly Gly Phe Ser  Ser Gln Gln Phe Ala  Tyr Phe Phe 
    1160                 1165                 1170             


Ile Tyr  Glu Ala Lys Lys Lys  Gly Lys Pro Thr Phe  Arg Phe Ser 
    1175                 1180                 1185             


Gln Val  Pro Val Trp Leu Ala  Ala Arg Ile Glu Ala  Asn Pro Leu 
    1190                 1195                 1200             


Ala Leu  Glu Glu Tyr Ala Arg  Gly Leu Ala Lys Gly  Glu Gly Leu 
    1205                 1210                 1215             


Glu Phe  Val Ser Ile Gln Arg  Pro Lys Ile Tyr Lys  Lys Gln Leu 
    1220                 1225                 1230             


Ile Glu  Val Asn Gly Asp Lys  Leu Ile Ile Thr Gly  Ala Lys Glu 
    1235                 1240                 1245             


Val Arg  Ser Gly Ala Glu Leu  Ala Phe Asn Gln Asp  Glu Ile Ser 
    1250                 1255                 1260             


Ala Ile  Ser Met Cys Ser Lys  Leu Arg Ala Ala Glu  Ala Gly Leu 
    1265                 1270                 1275             


Asp Gly  Ala Phe Ser Glu Glu  Val Ser Ala Ile Trp  Asn Arg Ile 
    1280                 1285                 1290             


Ser Asn  Ala Pro Val Gln Ser  Cys Gly Arg Leu Leu  Arg Gln Leu 
    1295                 1300                 1305             


Thr Leu  Pro Lys Tyr Gln Ser  Asn Phe Asp Ser Ser  Ser Asn Glu 
    1310                 1315                 1320             


Glu Lys  Ala Ala Val Val Leu  Gly Leu Ile Ala Leu  Leu Asn Gly 
    1325                 1330                 1335             


Asn Asn  Arg Ile Val Asp Leu  Ser Gly Val Gly Gly  Ser Ser His 
    1340                 1345                 1350             


Ala Gly  Ala Met Met Met Ser  Tyr Pro Lys Leu Leu  Asp Asp Pro 
    1355                 1360                 1365             


Lys Val  Pro Phe Phe Ile Ile  Asp Gln Ser Val Thr  Gly Met Phe 
    1370                 1375                 1380             


Glu Arg  Lys Thr Arg Val Gly  Leu 
    1385                 1390     


<210>  1135
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1135

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Lys Glu Asn Asn Glu Glu Gln Ser Ser Ile Ile 
            20                  25                  30          


Arg Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Lys Ser Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Asp Ile Thr Thr Asn Ala Asp Arg Thr Lys Cys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Phe Lys Leu Arg Arg Glu Ala 
65                  70                  75                  80  


Leu Ala Asp Cys Leu Lys Arg His Gly Leu Met Thr Asp Glu Thr Pro 
                85                  90                  95      


Leu Cys Glu Thr Asn Asn Ser Ile Ile Phe Lys Thr Tyr Ser Asn Arg 
            100                 105                 110         


Ala Lys Ala Val Cys Glu Lys Ile Ala Leu Asp Asp Phe Val Arg Val 
        115                 120                 125             


Leu Phe Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Leu 
    130                 135                 140                 


Asn Thr Asp Asp Glu Gly Thr Leu Val Asp Gly Met Asp Val Ala Lys 
145                 150                 155                 160 


Leu Leu Tyr Asn Glu Gln Ile Thr Pro Gly Glu Tyr Leu Leu Arg Leu 
                165                 170                 175     


Phe Lys Lys Gly Lys Lys Thr Arg Pro Asp Phe Tyr Leu Ser Asp Leu 
            180                 185                 190         


Lys Ala Glu Leu Glu Arg Ile Trp Asp Phe Gln Arg Thr Phe Tyr Pro 
        195                 200                 205             


Glu Ile Leu Cys Asp Ala Leu Lys Glu Asp Leu Lys Gly Lys Ser Arg 
    210                 215                 220                 


Thr Gln Ser Ser Ala Ile Phe Tyr Ala Lys Tyr Lys Val Ser Thr Ala 
225                 230                 235                 240 


Lys Ile Ala Gly Lys Asp Lys Arg Leu Gln Glu Leu Thr Trp Arg Thr 
                245                 250                 255     


Asp Ala Leu Arg Lys Gln Leu Thr Ile Asp Glu Val Ala Tyr Val Ile 
            260                 265                 270         


Cys Ser Ile Asn Gly Glu Ile Asn Gly Ser Ser Gln Arg Leu Ser Met 
        275                 280                 285             


Ile Ser Asp Arg Ser Lys Leu Leu Tyr Phe Asn His Gln Thr Ile Gly 
    290                 295                 300                 


Gln Tyr Leu Met Gln Arg Leu Glu Glu Asn Pro Asn Ala Gly Leu Lys 
305                 310                 315                 320 


Asn Ile Ile Phe Tyr Arg Gln Asp Tyr Glu Asp Glu Phe Asn Asp Ile 
                325                 330                 335     


Trp Asn Ser Gln Ala Thr Tyr His Lys Glu Leu Thr Pro Glu Leu Lys 
            340                 345                 350         


Lys Glu Leu Lys Asp Ile Ile Phe Tyr Gln Arg Gly Leu Lys Ser Cys 
        355                 360                 365             


Lys Trp Leu Ile Asn Tyr Cys Glu Leu Glu His Glu Glu Ile Glu Val 
    370                 375                 380                 


Lys Thr Pro Glu Gly Lys Leu Arg Lys Ile Thr Ile Gly Ser Lys Thr 
385                 390                 395                 400 


Cys Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Val 
                405                 410                 415     


Leu Asn Asn Leu Gln Val Ser Pro Leu Asn Ser Arg Arg Lys Ala Lys 
            420                 425                 430         


Arg Lys Lys Asp Glu Glu Pro Ser Leu Phe Ser Asp Glu Asp Met Asn 
        435                 440                 445             


Thr Ala Cys Arg Tyr Leu Asn Glu Glu Glu Lys Leu Thr Leu Phe Lys 
    450                 455                 460                 


Glu Leu Ser Ile Lys Lys Glu Leu Lys Lys Lys Asp Ala Leu Lys Leu 
465                 470                 475                 480 


Leu Phe Glu Asn Tyr Lys Glu Leu Asp Leu Asn Tyr Lys Val Ile Ser 
                485                 490                 495     


Gly Asn Val Thr Met Ala Ser Leu Tyr Arg Ala Phe Gln Asp Ile Ile 
            500                 505                 510         


Ser Leu Thr Gly Asn Gly Glu Phe Asp Phe Gln Ala Met Pro Ala Glu 
        515                 520                 525             


Glu Ala Glu Lys Thr Val Glu Glu Ile Phe Gly Gly Leu Gly Tyr Lys 
    530                 535                 540                 


Thr Ala Phe Leu His Phe Asp Ala Ser Leu His Gly Lys Glu Phe Glu 
545                 550                 555                 560 


Arg Gln Pro Ala Tyr Arg Leu Trp His Leu Leu Tyr Ser Tyr Ala Gly 
                565                 570                 575     


Asp Gln Ser Lys Thr Gly Ile Glu Ala Leu Val Lys Arg Ile Gln Glu 
            580                 585                 590         


Leu Cys Gly Phe Glu Pro Glu Tyr Ala Arg Ile Leu Ala Asn Ile Ser 
        595                 600                 605             


Phe Ile Pro Asp Tyr Gly Asn Leu Ser Ser Lys Ala Met Arg Lys Ile 
    610                 615                 620                 


Leu Pro Tyr Met Gln Asp Gly Tyr Glu Tyr Ser Glu Ala Cys Ser Lys 
625                 630                 635                 640 


Ala Gly Tyr Arg His Ser Ala Ser Ser Leu Thr Lys Glu Glu Ile Asn 
                645                 650                 655     


Lys Lys Thr Tyr Lys Thr His Leu Asp Glu Ile Lys Arg Asn Ser Leu 
            660                 665                 670         


Arg Asn Pro Ile Val Glu Lys Ile Leu Asn Gln Met Val Asn Val Val 
        675                 680                 685             


Asn Ser Val Ile Asp Thr Tyr Gly Ser Ile Asp Glu Ile Arg Ile Glu 
    690                 695                 700                 


Met Ala Arg Glu Leu Lys Lys Ser Lys Pro Glu Arg Glu Asp Met Ser 
705                 710                 715                 720 


Phe Ala Ile Asn Lys Ala Thr Lys Glu Thr Glu Arg Ile Lys Gly Ile 
                725                 730                 735     


Leu Glu Gln Glu Phe Lys Ile Gln His Val Thr Arg Asn Asp Ile Ile 
            740                 745                 750         


Arg Tyr Arg Leu Tyr Glu Glu Leu Lys Pro Asn Gly Tyr Lys Thr Leu 
        755                 760                 765             


Tyr Ser Asn Thr Tyr Ile Pro Arg Glu Asp Leu Phe Ser Lys Lys Phe 
    770                 775                 780                 


Asp Ile Glu His Ile Ile Pro Lys Ala Arg Leu Phe Asp Asp Ser Tyr 
785                 790                 795                 800 


Ser Asn Lys Thr Leu Glu Leu Arg Asp Ile Asn Ile Glu Lys Gly Asn 
                805                 810                 815     


Gln Thr Ala Tyr Asp Phe Val Leu Ser Lys Tyr Gly Glu Glu Gly Leu 
            820                 825                 830         


Tyr Lys Tyr Glu Gln Arg Val Glu Glu Phe Lys Lys Asn Asn Glu Gln 
        835                 840                 845             


Ala Gly Ser Lys Cys Arg Lys Leu Leu Met Arg Glu Ala Asp Ile Pro 
    850                 855                 860                 


Ser Asp Phe Leu Asn Arg Asp Leu Asn Asp Ser Gln Tyr Ile Ala Lys 
865                 870                 875                 880 


Lys Ala His Glu Leu Leu Arg Glu Ile Val Pro Thr Val Thr Ala Thr 
                885                 890                 895     


Thr Gly Ser Ile Thr Asn Arg Leu Arg Glu Asp Trp Lys Leu Val Asn 
            900                 905                 910         


Val Met Lys Glu Leu Asn Trp Glu Lys Tyr Asp Lys Leu Gly Leu Thr 
        915                 920                 925             


Glu Glu Tyr Lys Asp Lys Asp Gly Asn Thr Val Arg His Ile Lys Asp 
    930                 935                 940                 


Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr 
945                 950                 955                 960 


Ile Ala Phe Thr Lys Pro Ser Phe Ile Gln Tyr Leu Asn Asn Leu Asn 
                965                 970                 975     


Ala Arg Ser Asn Lys Gly Gly Ser Ile Tyr Gly Ile Glu Gln Lys Glu 
            980                 985                 990         


Leu Glu Arg Glu Asn Asn Lys Leu  Val Phe Ile Pro Pro  Ile Asp Pro 
        995                 1000                 1005             


Lys Ile  Phe Arg Lys Gln Ala  Lys Glu His Leu Ser  Asn Ile Leu 
    1010                 1015                 1020             


Ile Ser  Ile Lys Ala Lys Asn  Lys Val Val Thr Lys  Asn Val Asn 
    1025                 1030                 1035             


Lys Thr  Lys Ser Lys Ser Gly  Thr Asn Arg Lys Val  Gln Leu Thr 
    1040                 1045                 1050             


Pro Arg  Gly Gln Leu His Leu  Glu Thr Val Tyr Gly  Lys Ile Arg 
    1055                 1060                 1065             


Thr Tyr  Lys Thr Glu Glu Ile  Lys Val Gly Thr Lys  Leu Thr Glu 
    1070                 1075                 1080             


Glu Leu  Ile Ala Lys Val Ala  Asp Lys Lys Ile Arg  Glu Ala Leu 
    1085                 1090                 1095             


Met Gln  Arg Leu Ser Glu Tyr  Gly Gly Asn Ile Lys  Lys Ala Phe 
    1100                 1105                 1110             


Gln Asn  Ile Ser Lys His Pro  Ile Trp Leu Asn Ser  Ser His Thr 
    1115                 1120                 1125             


Lys His  Val Pro Glu Lys Val  Lys Ile Val Phe Phe  Glu Asp Val 
    1130                 1135                 1140             


Phe Thr  Ile Arg Lys Ala Ile  Thr Pro Asp Ile Lys  Ile Asp Lys 
    1145                 1150                 1155             


Val Ile  Asn Ser Lys Ile Arg  Asn Ile Leu Gln Glu  Arg Leu Asn 
    1160                 1165                 1170             


Glu Phe  Gly Gly Glu Ala Ala  Lys Ala Phe Ser Asn  Leu Asp Gln 
    1175                 1180                 1185             


Asn Pro  Ile Trp Phe Asn Lys  Asp Lys Gly Ile Ala  Ile Lys Ser 
    1190                 1195                 1200             


Val Lys  Ile Arg Gly Ile Ala  Asn Gly Thr Ala Ile  Arg Val Lys 
    1205                 1210                 1215             


Lys Asp  Ile His Gly Asn Thr  Ile Ile Gly Asn Asn  Gly Tyr Pro 
    1220                 1225                 1230             


Ile Pro  Asn Asp Tyr Val Asn  Thr Gly Asn Asn His  His Val Ala 
    1235                 1240                 1245             


Ile Phe  Arg Asp Arg Asp Gly  Asn Leu Gln Glu His  Ile Val Ser 
    1250                 1255                 1260             


Phe Tyr  Glu Ala Thr Ala Arg  Ala Ile Gln Asn Leu  Pro Ile Val 
    1265                 1270                 1275             


Asp Arg  Asn Tyr Asn Ser Glu  Lys Asp Trp Glu Phe  Leu Phe Ser 
    1280                 1285                 1290             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Pro  Asn Thr Gly 
    1295                 1300                 1305             


Phe Asn  Pro Leu Glu Ile Asp  Leu Arg Asn Glu Glu  Asn Tyr Ala 
    1310                 1315                 1320             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Leu  Ser His Tyr 
    1325                 1330                 1335             


Tyr Tyr  Val Phe Arg His His  Leu Glu Thr Asn Val  Asp Thr Asn 
    1340                 1345                 1350             


Leu Gln  Leu Lys Asp Ile Thr  Trp Lys Arg Ile Lys  Ala Ile Asn 
    1355                 1360                 1365             


Asn Leu  Thr Gly Val Val Lys  Val Arg Ile Asp His  Leu Gly Arg 
    1370                 1375                 1380             


Ile Val  Asp Val Gly Glu Tyr  Lys 
    1385                 1390     


<210>  1136
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1136

Met Lys Lys Arg Ile Asp Asp Tyr Ile Leu Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asn Ser Cys Gly Trp Ala Val Thr Asp Lys Lys Asn Asn Leu Leu Lys 
            20                  25                  30          


Leu Arg Gly Lys Thr Ala Ile Gly Ser His Leu Phe Glu Glu Gly His 
        35                  40                  45              


Thr Ala Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Arg Leu Leu Glu Glu Ile Phe Ala Glu Pro 
65                  70                  75                  80  


Met Ala Glu Val Asp Pro Gly Phe Phe Val Arg Leu His Gln Ser Trp 
                85                  90                  95      


Val Ser Pro Leu Asp Lys Ser Arg Lys Lys Tyr Gln Ala Ile Val Phe 
            100                 105                 110         


Pro Thr Ala Lys Glu Asp Gln Glu Tyr Tyr Gln Lys Tyr Pro Thr Ile 
        115                 120                 125             


Tyr His Leu Arg Asp Ala Leu Met Thr Gln Asp Arg Gln Phe Asp Leu 
    130                 135                 140                 


Arg Glu Ile Phe Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn 
145                 150                 155                 160 


Phe Leu Gln Asp Thr Ala Val Lys Asp Phe Glu Ala Ser Lys Ile Glu 
                165                 170                 175     


Val Gly Pro Ile Leu Thr Asn Ile Asn Asn Ala Phe Ala Asp Lys Ile 
            180                 185                 190         


Val Glu Gly Gln Asp Leu Ile Glu Leu Asn Val Ala Asn Ala Ala Glu 
        195                 200                 205             


Ile Glu Ala Val Ile Arg Gly Lys Asp Ala Asp Lys Thr Leu Tyr Lys 
    210                 215                 220                 


Met Asp Lys Val Lys Lys Ile Ala Lys Leu Leu Thr Asp Ser Thr Ala 
225                 230                 235                 240 


Lys Glu Gln Lys Asn Val Ala Lys Gln Ile Ala Asn Ala Ile Met Gly 
                245                 250                 255     


Tyr Lys Thr Gln Leu Glu Thr Ile Leu Gly Lys Glu Ile Asp Lys Asn 
            260                 265                 270         


Asp Lys Ala Gln Trp Glu Phe Lys Leu Ser Asp Ala Asp Asp Lys Leu 
        275                 280                 285             


Asp Ala Leu Leu Pro Asp Leu Asp Glu Ser Asp Gln Thr Val Val Ala 
    290                 295                 300                 


Glu Ile Glu Lys Leu Phe Ser Ala Ile Thr Leu Ser Thr Ile Val Asp 
305                 310                 315                 320 


Glu Asn Lys Ser Leu Ser Gln Ser Met Val Glu Lys Tyr Asn Lys His 
                325                 330                 335     


His Gln Asp Tyr Lys Leu Leu Lys Ala Tyr Ile Glu Ser Leu Pro Asp 
            340                 345                 350         


Arg Ala Gln Ala Lys Lys Leu Leu Leu Ala Tyr Asp Leu Tyr Val Asn 
        355                 360                 365             


Asn Arg His Gly His Leu Leu Glu Ala Lys Thr Glu Phe Lys Gln Asp 
    370                 375                 380                 


Ser Thr Leu Thr Lys Glu Asp Phe Tyr Lys Thr Val Lys Asp Asn Leu 
385                 390                 395                 400 


Asp Asp Ser Glu Leu Ala His Lys Ile Ala Gln Glu Ile Ala Ala Asp 
                405                 410                 415     


Asn Phe Met Pro Lys Gln Arg Thr Asn Ser Asn Gly Val Ile Pro Phe 
            420                 425                 430         


Gln Leu His Gln Ile Glu Leu Asp Lys Ile Ile Ala Asn Gln Ser Lys 
        435                 440                 445             


Tyr Tyr Pro Phe Leu Ala Ala Glu Asn Pro Val Glu Asp His Arg Lys 
    450                 455                 460                 


Gln Ala Pro Tyr Lys Leu Asp Glu Leu Val Arg Phe Arg Val Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Met Ile Thr Ala Asp Glu Gln Glu Lys Thr Ser Gly 
                485                 490                 495     


Lys Ser Phe Ala Trp Met Val Arg Lys Glu Gln Gly Gln Ile Thr Pro 
            500                 505                 510         


Trp Asn Phe Glu Gln Lys Val Asp Arg Gln Glu Ser Ala Asn Lys Phe 
        515                 520                 525             


Ile Lys Arg Met Thr Ile Lys Asp Thr Tyr Leu Leu Gly Glu Asp Val 
    530                 535                 540                 


Leu Pro Ala Asn Ser Leu Leu Tyr Gln Arg Phe Glu Val Leu Asn Glu 
545                 550                 555                 560 


Leu Asn Asn Ile Arg Val Asn Gly Ser Arg Ile Ser Val Asp Leu Lys 
                565                 570                 575     


Gln Gln Ile Phe Asn Asp Leu Phe Glu Glu Lys Lys Thr Val Thr Glu 
            580                 585                 590         


Lys Ala Leu Ile Ser Tyr Leu Lys Gln Asn Leu His Leu Pro Thr Val 
        595                 600                 605             


Glu Ile Lys Gly Leu Ala Asp Pro Thr Lys Phe Asn Ser Ser Leu Ala 
    610                 615                 620                 


Ser Tyr Tyr His Leu Lys Asn Leu His Val Phe Asp Lys Glu Leu Ala 
625                 630                 635                 640 


Asp Pro Gln Tyr Gln Lys Glu Phe Glu Lys Ile Ile Glu Tyr Ser Ser 
                645                 650                 655     


Ile Phe Glu Asp Lys Lys Ile Phe Gln Asp Lys Leu His Ala Glu Phe 
            660                 665                 670         


Lys Trp Leu Thr Pro Ala Gln Phe Lys Ala Ile Ser Thr Trp Arg Leu 
        675                 680                 685             


Gln Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Val Glu Leu His Asp 
    690                 695                 700                 


Thr Asn Gly Gln Asn Ile Ile Glu Gln Leu Trp Asp Ser Gln Lys Asn 
705                 710                 715                 720 


Phe Met Gln Ile Val Thr Glu Pro Asp Phe Lys Glu Ala Ile Ala Lys 
                725                 730                 735     


Glu Asn Gln Asn Ile Thr Gln Glu Asn Gly Val Glu Glu Ile Leu Ala 
            740                 745                 750         


Asp Ala Tyr Thr Ser Pro Ala Asn Lys Lys Ala Ile Arg Gln Val Val 
        755                 760                 765             


Lys Val Val Ala Asp Val Val Lys Ala Ala Ser Gly Lys Ala Pro Ala 
    770                 775                 780                 


Gln Phe Ala Ile Glu Phe Thr Arg Asp Pro Asp Lys Asn Pro Gln Leu 
785                 790                 795                 800 


Ser His Ile Arg Gly Thr Lys Leu Leu Lys Ala Tyr Gln Glu Thr Ala 
                805                 810                 815     


Gly Glu Leu Val Asp Gln Asn Leu Thr Asn Ser Leu Lys Glu Ala Met 
            820                 825                 830         


Thr Ser Arg Lys Leu Leu Lys Asp Lys Tyr Phe Leu Tyr Phe Met Gln 
        835                 840                 845             


Thr Gly Arg Asp Ala Tyr Thr Gly Lys Lys Ile Asn Ile Asp Glu Val 
    850                 855                 860                 


Ser Thr Asn Tyr Gln Ile Asp His Ile Leu Pro Gln Ser Phe Ile Lys 
865                 870                 875                 880 


Asp Asp Ser Phe Asp Asn Arg Val Leu Thr Ala Thr Pro Leu Asn Ala 
                885                 890                 895     


Glu Lys Ser Asp Asp Val Pro Tyr Lys Arg Phe Ala Asn Asn Tyr Val 
            900                 905                 910         


Ser Asp Leu Lys Met Thr Val Gly Glu Met Trp Lys His Trp Gln Lys 
        915                 920                 925             


Ala Gly Ile Ile Asn Lys His Lys Leu Gly Asn Leu Leu Leu Asp Pro 
    930                 935                 940                 


Asp Arg Leu Ser Lys Phe Gln Lys Ser Gly Phe Ile Asn Arg Gln Leu 
945                 950                 955                 960 


Val Glu Thr Ser Gln Ile Val Lys Leu Val Ser Val Ile Leu Gln Asn 
                965                 970                 975     


Lys Tyr Pro Asp Ala Glu Ile Ile Thr Val Lys Ala Gly Asp Asn Ser 
            980                 985                 990         


Ala Leu Arg Gln Arg Leu Asp Leu  Tyr Lys Ser Arg Asp  Val Asn Asp 
        995                 1000                 1005             


Tyr His  His Ala Ile Asp Ala  Tyr Leu Ser Ile Val  Cys Gly Asn 
    1010                 1015                 1020             


Phe Leu  Tyr Gln Val Tyr Pro  Lys Tyr Arg Pro Tyr  Phe Val Tyr 
    1025                 1030                 1035             


Gly Gln  Phe Lys Lys Phe Ser  Gln Asp Pro Asp Leu  Gln Lys Asp 
    1040                 1045                 1050             


Leu Ile  Lys His Phe Lys Gly  Phe Thr Phe Met Trp  Pro Leu Leu 
    1055                 1060                 1065             


Gln Lys  Asp Asn Ser Glu Arg  Lys Ala Leu Glu Glu  Ile Lys Glu 
    1070                 1075                 1080             


Asn Asn  Ser Asp Arg Ile Val  Phe Tyr Lys His Pro  Asp Ile Phe 
    1085                 1090                 1095             


Asp Lys  Leu Arg Lys Ala Tyr  Asn Tyr Lys Tyr Met  Leu Val Ser 
    1100                 1105                 1110             


Arg Glu  Thr Ser Thr Glu Ser  Ser Ser Met Phe Asn  Met Thr Ile 
    1115                 1120                 1125             


Tyr Pro  Arg Gly Glu Arg Asp  Arg Lys Lys Thr Arg  Lys Leu Ile 
    1130                 1135                 1140             


Pro Lys  Gly Asn Gly Leu Asp  Pro Lys Ile Tyr Gly  Gly Tyr Ser 
    1145                 1150                 1155             


Gly Asn  Thr Asp Ala Tyr Met  Val Ile Val Lys Ile  Glu Lys Ala 
    1160                 1165                 1170             


Lys Glu  Ser Ile Tyr Lys Val  Ile Gly Ile Pro Met  Arg Ala Leu 
    1175                 1180                 1185             


Ala Gly  Leu Lys Gln Ala Lys  Asn Gln Gly Asn Tyr  Lys Glu Glu 
    1190                 1195                 1200             


Leu His  Lys Val Val Glu Pro  Gln Ile Met Leu Asp  Lys Asn Gly 
    1205                 1210                 1215             


Lys Pro  Lys Arg Gly Val Glu  Gly Phe Arg Val Val  Lys Asp His 
    1220                 1225                 1230             


Val Pro  Phe Lys Gln Val Val  Leu Asp Gly Asp Lys  Lys Phe Met 
    1235                 1240                 1245             


Leu Tyr  Ser Ala Lys Tyr Glu  Thr Asn Ala Lys Gln  Leu Thr Leu 
    1250                 1255                 1260             


Ser Gln  Glu Thr Met Arg Ile  Val Thr Asp Asn Leu  Lys Lys Ser 
    1265                 1270                 1275             


Glu Asp  Gln Asp Lys Leu Leu  Val Lys Ala Tyr Asp  Glu Ile Leu 
    1280                 1285                 1290             


Gln Lys  Val Asp Gln Tyr Leu  Pro Leu Phe Asp Val  Asn Lys Phe 
    1295                 1300                 1305             


Arg Asn  Ser Leu His Leu Gly  Arg Ala Lys Phe Leu  Asp Leu Ala 
    1310                 1315                 1320             


Val Asn  Asp Lys Lys Ile Thr  Leu Ser Asn Ile Leu  Asn Gly Leu 
    1325                 1330                 1335             


His Asp  Asn Leu Val Val Pro  Asn Leu Lys Asn Ile  Gly Ile Lys 
    1340                 1345                 1350             


Thr Pro  Phe Gly Leu Met Gln  Ser Pro Ser Gly Ile  Thr Leu Ser 
    1355                 1360                 1365             


Ser Asn  Ala Ile Leu Ile Phe  Gln Ser Pro Thr Gly  Leu Phe Glu 
    1370                 1375                 1380             


Lys Arg  Val Arg Ile Ala Asp  Leu 
    1385                 1390     


<210>  1137
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1137

Met Glu Asn Lys Gln Tyr Tyr Ile Gly Leu Asp Val Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Thr Asp Thr Ser Tyr Asn Leu Leu Arg Ala Lys 
            20                  25                  30          


Gly Lys Asp Met Trp Gly Ala Arg Leu Phe Asp Glu Ala Lys Thr Ala 
        35                  40                  45              


Val Glu Arg Arg Thr Lys Arg Thr Ser Arg Arg Arg Ser Glu Arg Glu 
    50                  55                  60                  


Lys Ala Arg Lys Ala Met Leu Lys Glu Leu Phe Ala Asp Glu Ile Asn 
65                  70                  75                  80  


Lys Val Asp Pro Asn Phe Phe Ile Arg Leu Glu Glu Ser Lys Phe Phe 
                85                  90                  95      


Leu Asp Asp Arg Ser Glu Asn Asn Arg Gln Arg Tyr Thr Leu Phe Asn 
            100                 105                 110         


Asp Ala Lys Phe Thr Asp Lys Asp Tyr Tyr Glu Lys Tyr Lys Thr Ile 
        115                 120                 125             


Phe His Leu Arg Ser Ala Leu Ile Asn Ser Asp Glu Lys Phe Asp Val 
    130                 135                 140                 


Arg Leu Val Phe Leu Ala Ile Leu Asn Leu Phe Ser His Arg Gly His 
145                 150                 155                 160 


Phe Leu Asn Ala Ser Leu Lys Gly Asp Gly Glu Ile Gln Gly Val Asp 
                165                 170                 175     


Ile Phe Tyr Asn Asp Leu Tyr Glu Val Cys Glu Glu Phe Glu Ile Asp 
            180                 185                 190         


Leu Pro Gln Ile Ser Asn Ile Asp Asn Phe Glu Lys Ile Leu Ser Gln 
        195                 200                 205             


Lys Gly Lys Ser Arg Thr Lys Ile Leu Glu Glu Leu Ser Glu Glu Leu 
    210                 215                 220                 


Ser Ile Ser Lys Lys Glu Lys Ile Lys Tyr Ser Leu Leu Lys Leu Ile 
225                 230                 235                 240 


Cys Gly Met Lys Ile Lys Val Val Asp Ile Tyr Ala Asp Glu Asp Ile 
                245                 250                 255     


Gln Glu Asp Asp Lys Lys Lys Asp Leu Gly Phe Arg Glu Ser Asp Tyr 
            260                 265                 270         


Glu Lys Ser Ser Leu Glu Val Lys Glu Ile Ile Gly Asp Asp His Phe 
        275                 280                 285             


Glu Leu Ile Glu Arg Ala Lys Ala Val His Asp Ile Gly Leu Leu Ala 
    290                 295                 300                 


Asn Ile Ile Gly Asn Ser Lys Tyr Leu Cys Glu Ala Arg Val Glu Ala 
305                 310                 315                 320 


Tyr Asp Asn His His Lys Asp Leu Gln Lys Ile Lys Glu Leu Leu Lys 
                325                 330                 335     


Lys Tyr Asp Ile Lys Ala Tyr Thr Asp Met Phe Arg Lys Met Thr Asp 
            340                 345                 350         


Lys Asn Tyr Ser Ala Tyr Val Gly Ser Val Asn Ser Asn Ile Ala Lys 
        355                 360                 365             


Glu Arg Arg Ser Val Asp Asn Arg Lys Ile Glu Asp Leu Tyr Glu Tyr 
    370                 375                 380                 


Ile Lys Lys Ile Ala Leu Lys Asn Ile Ser Asp Asp Asn Glu Asp Lys 
385                 390                 395                 400 


Ile Glu Ile Leu Lys Lys Ile Glu Thr Gly Glu Phe Leu Lys Lys Gln 
                405                 410                 415     


Leu Thr Ala Ser Asn Gly Val Ile Pro Asn Gln Leu Gln Ser Arg Glu 
            420                 425                 430         


Leu Arg Ala Ile Leu Lys Lys Ala Glu Asn Tyr Leu Pro Phe Leu Lys 
        435                 440                 445             


Glu Lys Gly Glu Lys Asn Leu Thr Val Ser Glu Met Ile Ile Gln Leu 
    450                 455                 460                 


Phe Glu Phe Gln Ile Pro Tyr Tyr Val Gly Pro Leu Asp Lys Asn Pro 
465                 470                 475                 480 


Lys Lys Asp Lys Asn Ala Asn Ser Trp Ala Val Ile Pro Ser Gly Gly 
                485                 490                 495     


Arg Ile Leu Pro Trp Asn Phe Glu Asp Lys Val Asp Val Lys Gly Ser 
            500                 505                 510         


Arg Lys Glu Phe Ile Glu Lys Met Val Arg Lys Cys Thr Tyr Leu Ser 
        515                 520                 525             


Glu Glu His Thr Leu Pro Lys Gln Ser Leu Leu Tyr Glu Lys Phe Met 
    530                 535                 540                 


Val Leu Asn Glu Ile Asn Asn Ile Lys Ile Asp Gly Glu Lys Ile Ser 
545                 550                 555                 560 


Val Glu Ala Lys Gln Lys Ile Tyr Asn Asp Leu Phe Met Lys Gly Lys 
                565                 570                 575     


Lys Val Ser Gln Lys Ala Ile Lys Gln Glu Leu Ile Ser Leu Asn Ile 
            580                 585                 590         


Met Ser Lys Asp Ser Val Leu Ser Gly Ile Asp Thr Val Cys Asn Ala 
        595                 600                 605             


Tyr Leu Ser Ser Ile Ala Lys Phe Arg Gly Val Phe Asp Glu Asn Val 
    610                 615                 620                 


Asn Asn Gln Ser Thr Ile Asp Met Ile Glu Asp Ile Ile Phe Leu Lys 
625                 630                 635                 640 


Thr Val Tyr Ser Asp Glu Lys Arg Phe Val Lys Glu Glu Ile Val Glu 
                645                 650                 655     


Lys Tyr Gly Asp Glu Ile Asp Lys Asp Lys Ile Lys Arg Ile Leu Gly 
            660                 665                 670         


Phe Lys Phe Ser Asn Trp Gly Asn Ile Ser Lys Ser Phe Leu Glu Leu 
        675                 680                 685             


Glu Gly Ala Asp Val Gly Thr Gly Glu Ile Arg Ser Ile Ile Gln Ser 
    690                 695                 700                 


Leu Trp Glu Thr Asn Phe Asn Leu Met Glu Leu Leu Ser Ser Arg Phe 
705                 710                 715                 720 


Thr Tyr Met Asp Glu Leu Glu Lys Arg Val Lys Lys Leu Glu Lys Pro 
                725                 730                 735     


Leu Ser Glu Trp Thr Ile Glu Asp Leu Asp Asp Met Tyr Leu Ser Ser 
            740                 745                 750         


Pro Val Lys Arg Met Ile Trp Gln Ser Met Lys Ile Val Asp Glu Ile 
        755                 760                 765             


Gln Thr Val Ile Gly Tyr Ala Pro Lys Arg Ile Phe Val Glu Met Thr 
    770                 775                 780                 


Arg Ser Glu Gly Glu Lys Val Arg Thr Lys Ser Arg Lys Asp Arg Leu 
785                 790                 795                 800 


Lys Glu Leu Tyr Ser Ala Ile Lys Glu Asp Ser Lys Gln Trp Val Lys 
                805                 810                 815     


Glu Leu Asp Ala Lys Asp Glu Ser Tyr Phe Arg Ser Lys Lys Met Tyr 
            820                 825                 830         


Leu Tyr Tyr Leu Gln Lys Gly Arg Cys Met Tyr Ser Gly Glu Val Ile 
        835                 840                 845             


Glu Leu Asp Lys Leu Met Asp Asp Asn Leu Tyr Asp Ile Asp His Ile 
    850                 855                 860                 


Tyr Pro Arg Ser Phe Val Lys Asp Asp Ser Leu Asp Asn Leu Val Leu 
865                 870                 875                 880 


Val Lys Lys Glu Ile Asn Asn Arg Lys Gln Asn Asp Pro Ile Thr Pro 
                885                 890                 895     


Gln Ile Gln Ala Ser Cys Gln Gly Phe Trp Lys Val Leu His Glu Gln 
            900                 905                 910         


Gly Phe Ile Ser Asn Glu Lys Tyr Ser Arg Leu Thr Arg Lys Thr Gln 
        915                 920                 925             


Glu Phe Ser Asp Glu Glu Lys Leu Ser Phe Ile Asn Arg Gln Ile Val 
    930                 935                 940                 


Glu Thr Gly Gln Ala Thr Lys Cys Met Ala Gln Ile Leu Gln Lys Ser 
945                 950                 955                 960 


Met Gly Glu Asp Val Asp Val Val Phe Ser Lys Ala Arg Leu Val Ser 
                965                 970                 975     


Glu Phe Arg His Lys Phe Glu Leu Phe Lys Ser Arg Leu Ile Asn Asp 
            980                 985                 990         


Phe His His Ala Asn Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Ser 
        995                 1000                 1005             


Tyr Phe  Val Lys Phe Thr Arg  Asn Pro Ala Asn Phe  Ile Lys Asp 
    1010                 1015                 1020             


Ala Arg  Lys Asn Pro Asp Asn  Pro Ala Tyr Lys Tyr  His Met Asp 
    1025                 1030                 1035             


Arg Phe  Phe Glu Arg Asp Val  Lys Ser Lys Ser Glu  Val Ala Trp 
    1040                 1045                 1050             


Ile Gly  Gln Ser Glu Gly Asn  Ala Gly Thr Ile Ala  Ile Val Lys 
    1055                 1060                 1065             


Lys Thr  Met Ser Lys Asn Ser  Pro Leu Ile Thr Lys  Lys Val Glu 
    1070                 1075                 1080             


Glu Gly  His Gly Ser Ile Thr  Lys Glu Thr Ile Val  Gly Val Lys 
    1085                 1090                 1095             


Glu Ile  Lys Phe Gly Arg Asn  Lys Val Glu Lys Ala  Asp Lys Thr 
    1100                 1105                 1110             


Pro Lys  Lys Ala Asn Leu Gln  Ala Tyr Arg Pro Ile  Lys Thr Ser 
    1115                 1120                 1125             


Asp Glu  Arg Leu Cys Asn Ile  Leu Arg Tyr Gly Gly  Arg Thr Ser 
    1130                 1135                 1140             


Ile Ser  Ile Ser Gly Tyr Cys  Leu Val Glu Tyr Val  Lys Lys Arg 
    1145                 1150                 1155             


Lys Thr  Ile Arg Ser Leu Glu  Ala Ile Pro Val Tyr  Leu Gly Arg 
    1160                 1165                 1170             


Lys Asp  Ser Leu Ser Glu Glu  Lys Leu Leu Asn Tyr  Phe Arg Tyr 
    1175                 1180                 1185             


Asn Leu  Asn Asp Gly Gly Lys  Asp Ser Val Ser Asp  Ile Arg Leu 
    1190                 1195                 1200             


Cys Leu  Pro Phe Ile Ser Thr  Asn Ser Leu Val Lys  Ile Asp Gly 
    1205                 1210                 1215             


Tyr Leu  Tyr Tyr Leu Gly Gly  Lys Asn Asp Asp Arg  Ile Gln Leu 
    1220                 1225                 1230             


Tyr Asn  Ala Tyr Gln Leu Lys  Met Lys Lys Glu Glu  Val Glu Tyr 
    1235                 1240                 1245             


Ile Arg  Lys Ile Glu Lys Ala  Val Ser Met Ser Lys  Phe Asp Glu 
    1250                 1255                 1260             


Ile Asp  Arg Glu Lys Asn Pro  Val Leu Thr Glu Glu  Lys Asn Ile 
    1265                 1270                 1275             


Glu Leu  Tyr Asn Lys Ile Gln  Asp Lys Phe Glu Asn  Thr Val Phe 
    1280                 1285                 1290             


Ser Lys  Arg Met Ser Leu Val  Lys Tyr Asn Lys Lys  Asp Leu Ser 
    1295                 1300                 1305             


Phe Gly  Asp Phe Leu Lys Asn  Lys Lys Ser Lys Phe  Glu Glu Ile 
    1310                 1315                 1320             


Asp Leu  Glu Lys Gln Cys Lys  Val Leu Tyr Asn Ile  Ile Phe Asn 
    1325                 1330                 1335             


Leu Ser  Asn Leu Lys Glu Val  Asp Leu Ser Asp Ile  Gly Gly Ser 
    1340                 1345                 1350             


Lys Ser  Thr Gly Lys Cys Arg  Cys Lys Lys Asn Ile  Thr Asn Tyr 
    1355                 1360                 1365             


Lys Glu  Phe Lys Leu Ile Gln  Gln Ser Ile Thr Gly  Leu Tyr Ser 
    1370                 1375                 1380             


Cys Glu  Lys Asp Leu Met Thr  Ile 
    1385                 1390     


<210>  1138
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1138

Met Lys Arg Asn Asn Asp Tyr Tyr Ile Gly Leu Asp Met Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Ala Thr Thr Asn Pro Gln Gly Arg Leu Leu Arg Phe 
            20                  25                  30          


Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Glu Glu Ala Lys Thr 
        35                  40                  45              


Ala Ala Lys Thr Arg Thr Phe Arg Thr Ser Arg Arg Arg Tyr Asp Arg 
    50                  55                  60                  


Val Arg Gln Arg Ile Glu Phe Leu Gln Asp Ile Phe Ala Pro Ile Ile 
65                  70                  75                  80  


Ala Lys Lys Asp Phe Glu Phe Phe Met Arg Leu Lys Glu Ser Lys Phe 
                85                  90                  95      


His Leu Glu Asp Lys Lys Thr Asp Gly Lys Phe Ala Leu Phe Asn Asp 
            100                 105                 110         


Ala Asp Phe Thr Asp Val Glu Tyr Phe Asp Arg Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Ala Ala Leu Ile Lys Pro Asn His Ile Thr Asp Pro Arg 
    130                 135                 140                 


Leu Leu Tyr Leu Ala Ile His His Ile Ile Lys Asn Arg Gly His Phe 
145                 150                 155                 160 


Leu Phe Ala Gly Thr Asp Met Glu Ser Ile Arg Ser Phe Gln Ile Val 
                165                 170                 175     


Tyr Thr Gln Leu Ser Glu His Leu Lys Ser Ile Asn Leu Asp Phe Glu 
            180                 185                 190         


Leu Pro Val Asp Ala Gln Asp Lys Phe Ala Ala Ile Leu Leu Asn Glu 
        195                 200                 205             


Lys Leu Asn Lys Arg Asp Lys Lys Lys Gln Leu Lys Glu Leu Cys Pro 
    210                 215                 220                 


His Lys Asp Lys Arg Leu Glu Tyr Ala Phe Asn Ala Ile Ile Gly Gly 
225                 230                 235                 240 


Lys Thr Lys Leu Lys Asp Ile Phe Gly Val Glu Glu Tyr Asp Glu Gly 
                245                 250                 255     


Thr Lys Lys Ala Val Asp Phe Glu Lys Ala Asp Tyr Asp Glu Asp Lys 
            260                 265                 270         


Asp Glu Ile Glu Ser Val Ile Lys Glu Asn Ile His Val Leu Asn Ala 
        275                 280                 285             


Leu Lys Ala Val Tyr Asp Trp Thr Ile Leu Thr Asn Ile Leu Gly Asp 
    290                 295                 300                 


Tyr Ala Tyr Ile Ser Glu Ser Gln Val Ala Leu Tyr Glu Ala His Lys 
305                 310                 315                 320 


Lys Asp Leu Arg Ile Leu Lys Ser Leu Ile Lys Lys Tyr Ala Lys Ser 
                325                 330                 335     


Gln Tyr Ala Asp Ala Phe Arg His Glu Asn Val Lys Gly Asn Tyr Ala 
            340                 345                 350         


Asn Tyr Val Gly Ser Thr Asn Tyr Asp Asn Lys Gln Pro Val Ile Asp 
        355                 360                 365             


Asn Pro Ser Val Thr Gln Glu Asp Ile Asn Lys Tyr Phe Ala Asp Ile 
    370                 375                 380                 


Leu Leu Ser Ile Thr Pro Asp Glu Ser Asp Arg Glu Ser Tyr Gln Tyr 
385                 390                 395                 400 


Ile Thr Glu Arg Leu His Lys Gly Glu Ala Leu Pro Lys Leu Arg Thr 
                405                 410                 415     


Lys Ala Asn Ser Val Leu Pro His Gln Ile His Lys Ile Glu Leu Glu 
            420                 425                 430         


Lys Ile Leu Ala Asn Ala Ala Lys Asn Phe Pro Phe Leu Gln Glu Lys 
        435                 440                 445             


Asp Ser Ser Gly Tyr Thr Ala Ala Gln Lys Ile Val Lys Ile Met Met 
    450                 455                 460                 


Phe Arg Ile Glu Tyr Tyr Val Gly Pro Leu Asn Thr Tyr His His Val 
465                 470                 475                 480 


Asp Gln Gly Gly His Ala Trp Met Val Arg Lys Ala Glu Gly Lys Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Glu Lys Val Asp Lys Glu Ala Ser Ala Glu 
            500                 505                 510         


Lys Phe Ile Arg Asn Met Thr Asn Lys Cys Thr Tyr Leu Ile Gly Lys 
        515                 520                 525             


Asp Val Ile Pro Lys Gln Ser Leu Leu Tyr Ser Arg Tyr Lys Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Asn Leu Lys Val Asn Gly Ala Pro Ile Ser Val Ala 
545                 550                 555                 560 


Leu Lys Gln Lys Ile Tyr Glu Glu Leu Phe Lys Lys Gln Val Asn Val 
                565                 570                 575     


Thr Phe Asn Gln Leu Lys Thr Phe Leu Lys Thr Gln Gly Glu Asp Ala 
            580                 585                 590         


Asp Glu Ile Ile Leu Ser Gly Val Asp Glu Ala Ala His Lys Phe Thr 
        595                 600                 605             


Asn Asn Leu Gln Thr Tyr His Lys Phe Arg Ala Ile Leu Arg Asp Lys 
    610                 615                 620                 


Ile Glu Phe Glu Pro Gln Arg Ser Met Val Glu Asp Ile Ile Arg Trp 
625                 630                 635                 640 


Lys Cys Leu Met Gly Asp Asp Ile Thr Met Trp Lys His Lys Ile Glu 
                645                 650                 655     


Lys Glu Tyr Gly Asp Gln Leu Ser Glu Glu Gln Leu Lys Lys Ile Thr 
            660                 665                 670         


Arg Leu Asn Phe Asn Gly Trp Ser Arg Leu Ser Gln Glu Phe Leu Thr 
        675                 680                 685             


Asp Val Pro Gly Val Asp Ile Glu Ser Gly Glu Val Tyr Ser Gly Ile 
    690                 695                 700                 


Met Asp Ala Leu Trp Arg Thr Asn Glu Asn Ile Met Gln Leu Leu Ser 
705                 710                 715                 720 


Ser Arg Phe Thr Phe Ala Ala Glu Val Lys Lys Gln Asn Gln Gly Thr 
                725                 730                 735     


Gly Arg Ile Glu Lys Ile Thr Tyr Asp Glu Val Met Lys Asp Thr Tyr 
            740                 745                 750         


Leu Ser Pro Ala Val Lys Arg Ser Val Trp Gln Ala Ile Thr Leu Val 
        755                 760                 765             


Glu Glu Ile Arg Lys Ile Arg Lys Ala Asp Pro Lys Arg Ile Phe Val 
    770                 775                 780                 


Glu Met Thr Arg Gln Pro Asp Ala Lys Lys Glu Arg Lys Leu Ser Arg 
785                 790                 795                 800 


Arg Thr Gln Leu Leu Asn Ala Tyr Arg Ala Ile Gly Asn Asp Ile Ala 
                805                 810                 815     


Glu Tyr Glu Lys Glu His Val Ser Thr Leu Ile Thr Gln Leu Glu Ser 
            820                 825                 830         


Lys Lys Asp Glu Asp Leu Arg Arg Lys Lys Leu Tyr Leu Tyr Phe Thr 
        835                 840                 845             


Gln Met Gly Arg Cys Met Tyr Thr Gly Glu Arg Ile Asp Leu Ser Ser 
    850                 855                 860                 


Leu Leu Arg Asp Thr Asn Ser Asn Ile Tyr Asp Ile Asp His Ile Tyr 
865                 870                 875                 880 


Pro Arg Ser Leu Thr Lys Asp Asp Ser Leu Asp Asn Thr Val Leu Cys 
                885                 890                 895     


Lys Lys Glu Ile Asn Leu Lys Lys Gly Asn Lys Tyr Ile His Arg Val 
            900                 905                 910         


Phe Thr Asp Lys Glu Leu Lys Lys Gln Tyr Gly Tyr Trp Lys Ala Leu 
        915                 920                 925             


Lys Glu Lys Lys Leu Ile Ser Glu Arg Lys Phe Ser Arg Leu Met Arg 
    930                 935                 940                 


Thr Thr Asp Leu Thr Ala Asp Glu Leu Ala Gly Phe Ile Ser Arg Gln 
945                 950                 955                 960 


Leu Val Glu Thr Ser Gln Met Val Lys Ala Thr Ala Asp Ile Leu Glu 
                965                 970                 975     


Lys Ile Tyr Pro Asn Val Thr Ile Val Tyr Val Lys Ala Arg Thr Val 
            980                 985                 990         


Ser Asp Phe Arg His Asp Tyr Glu  Leu Pro Lys Val Arg  Ala Leu Asn 
        995                 1000                 1005             


Asp Cys  His His Ala His Asp  Ala Tyr Leu Asn Ile  Val Val Gly 
    1010                 1015                 1020             


Asn Val  Tyr Tyr Glu Lys Phe  Thr Lys Ser Pro Phe  Arg Phe Ile 
    1025                 1030                 1035             


Ala Asn  Gln Lys Gly Lys Gly  Asp Ile Lys Tyr Asn  Leu Tyr Arg 
    1040                 1045                 1050             


Leu Phe  Asp Lys Asp Val Lys  Val Gly Asp Arg Ile  Ile Trp Asn 
    1055                 1060                 1065             


Ala Lys  Glu Asp Leu Ala Arg  Val Lys Ala Thr Leu  Glu Arg Asp 
    1070                 1075                 1080             


Thr Val  Asn Val Val Arg Met  Pro Val Glu Gln Arg  Gly Gln Phe 
    1085                 1090                 1095             


Phe Asp  Ala Thr Ile Gln Lys  Lys Gly Thr Thr Lys  Asn Met Leu 
    1100                 1105                 1110             


Val Leu  Pro Leu Lys Gly Ser  Asp Glu Arg Leu Gln  Asp Thr Ser 
    1115                 1120                 1125             


Lys Tyr  Gly Tyr Tyr Val Arg  Pro Ser Gly Met Ser  Phe Met Val 
    1130                 1135                 1140             


Val Gln  His Glu Val Lys Gly  Lys Ser Arg Val Thr  Ile Glu Pro 
    1145                 1150                 1155             


Ala Leu  Leu Val Tyr Lys Asn  Lys Leu Lys Ser Glu  Asp Asp Tyr 
    1160                 1165                 1170             


Ala Lys  Tyr Cys Glu Asp Val  Leu Lys Leu Gln Lys  Pro Lys Val 
    1175                 1180                 1185             


Leu Phe  Lys Asn Ile Lys Phe  Gly Ser Lys Ile Leu  Phe Asp Gly 
    1190                 1195                 1200             


Tyr Pro  Phe Leu Leu Lys Gly  Arg Thr Glu Asn Arg  Ile Ile Met 
    1205                 1210                 1215             


Ala Asp  Ala Gln Ser Leu Phe  Leu Gly Asn Gln Glu  Leu Lys Ile 
    1220                 1225                 1230             


Leu Lys  Arg Val Thr Lys Ile  Ser Asp Asp Ile Asp  His Lys Val 
    1235                 1240                 1245             


Glu Ser  Arg Ile Lys Ile Thr  Asp Glu Glu Trp Thr  Thr Leu Trp 
    1250                 1255                 1260             


Lys Ser  Val Val Glu Gln Ile  Val Thr Gly Lys Phe  Arg Asn Arg 
    1265                 1270                 1275             


Leu Gly  Thr Leu Asn Gly Ile  Leu Gln Gly Arg Lys  Ile Lys Leu 
    1280                 1285                 1290             


Ser Lys  Asp Glu Glu Ala Arg  Leu Asn Glu Leu Asp  Ser Lys Asp 
    1295                 1300                 1305             


Gln Glu  Thr Phe Leu Ser Gln  Ile Leu Leu Glu Arg  Phe Leu Gln 
    1310                 1315                 1320             


Phe Ser  Thr Glu Glu Lys Thr  Arg Leu Leu Leu Gln  Val Ile Ser 
    1325                 1330                 1335             


Phe Ser  Gly Asn Thr Thr Gly  Val Asp Leu Arg Leu  Ile Gly Gly 
    1340                 1345                 1350             


Ser Ser  Gly Thr Gly Thr Ile  Lys Thr Asn Lys Asn  Ile Thr Asp 
    1355                 1360                 1365             


His Ser  Val Ile Leu Ile Asn  Glu Ser Pro Thr Gly  Leu Phe Thr 
    1370                 1375                 1380             


Asn Arg  Thr Val Leu Asn Gln  Pro 
    1385                 1390     


<210>  1139
<211>  1391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1139

Met Lys Arg Asn Asn Asp Tyr Tyr Ile Gly Leu Asp Met Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Ala Thr Thr Asn Pro Gln Gly Arg Leu Leu Arg Phe 
            20                  25                  30          


Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Glu Glu Ala Lys Thr 
        35                  40                  45              


Ala Ala Lys Thr Arg Thr Phe Arg Thr Ser Arg Arg Arg Tyr Asp Arg 
    50                  55                  60                  


Val Arg Gln Arg Ile Glu Phe Leu Gln Asp Ile Phe Ala Pro Ile Ile 
65                  70                  75                  80  


Ala Lys Lys Asp Phe Glu Phe Phe Met Arg Leu Lys Glu Ser Lys Phe 
                85                  90                  95      


His Leu Glu Asp Lys Lys Thr Asp Gly Lys Phe Ala Leu Phe Asn Asp 
            100                 105                 110         


Ala Asp Phe Thr Asp Val Glu Tyr Phe Asp Arg Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Ala Ala Leu Ile Lys Pro Asn His Ile Thr Asp Pro Arg 
    130                 135                 140                 


Leu Leu Tyr Leu Ala Ile His His Ile Ile Lys Asn Arg Gly His Phe 
145                 150                 155                 160 


Leu Phe Ala Gly Thr Asp Met Glu Ser Ile Arg Ser Phe Gln Ile Val 
                165                 170                 175     


Tyr Thr Gln Leu Ser Glu His Leu Lys Ser Ile Asn Leu Asp Phe Glu 
            180                 185                 190         


Leu Pro Val Asp Ala Gln Asp Lys Phe Ala Ala Ile Leu Leu Asn Glu 
        195                 200                 205             


Lys Leu Asn Lys Arg Asp Lys Lys Lys Gln Leu Lys Glu Leu Cys Pro 
    210                 215                 220                 


His Lys Asp Lys Arg Leu Glu Tyr Ala Phe Asn Ala Ile Ile Gly Gly 
225                 230                 235                 240 


Lys Thr Lys Leu Lys Asp Ile Phe Gly Val Glu Glu Tyr Asp Glu Gly 
                245                 250                 255     


Thr Lys Lys Ala Val Asp Phe Glu Lys Ala Asp Tyr Asp Glu Asp Lys 
            260                 265                 270         


Asp Glu Ile Glu Ser Val Ile Lys Glu Asn Ile His Val Leu Asn Ala 
        275                 280                 285             


Leu Lys Ala Val Tyr Asp Trp Thr Ile Leu Thr Asn Ile Leu Gly Asp 
    290                 295                 300                 


Tyr Ala Tyr Ile Ser Glu Ser Gln Val Ala Leu Tyr Glu Ala His Lys 
305                 310                 315                 320 


Lys Asp Leu Arg Ile Leu Lys Ser Leu Ile Lys Lys Tyr Ala Lys Ser 
                325                 330                 335     


Gln Tyr Ala Asp Ala Phe Arg His Glu Asn Val Lys Gly Asn Tyr Ala 
            340                 345                 350         


Asn Tyr Val Gly Ser Thr Asn Tyr Asp Asn Lys Gln Pro Val Ile Asp 
        355                 360                 365             


Asn Pro Ser Val Thr Gln Glu Asp Ile Asn Lys Tyr Phe Ala Asp Ile 
    370                 375                 380                 


Leu Leu Ser Ile Thr Pro Asp Glu Ser Asp Arg Glu Ser Tyr Gln Tyr 
385                 390                 395                 400 


Ile Thr Glu Arg Leu His Lys Gly Glu Ala Leu Pro Lys Leu Arg Thr 
                405                 410                 415     


Lys Ala Asn Ser Val Leu Pro His Gln Ile His Lys Ile Glu Leu Glu 
            420                 425                 430         


Lys Ile Leu Ala Asn Ala Ala Lys Asn Phe Pro Phe Leu Gln Glu Lys 
        435                 440                 445             


Asp Ser Ser Gly Tyr Thr Ala Ala Gln Lys Ile Val Lys Ile Met Met 
    450                 455                 460                 


Phe Arg Ile Glu Tyr Tyr Val Gly Pro Leu Asn Thr Tyr His His Val 
465                 470                 475                 480 


Asp Gln Gly Gly His Ala Trp Met Val Arg Lys Ala Glu Gly Lys Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Asp Glu Lys Val Asp Lys Glu Ala Ser Ala Glu 
            500                 505                 510         


Lys Phe Ile Arg Asn Met Thr Asn Lys Cys Thr Tyr Leu Ile Gly Lys 
        515                 520                 525             


Asp Val Ile Pro Lys Gln Ser Leu Leu Tyr Ser Arg Tyr Lys Val Leu 
    530                 535                 540                 


Asn Glu Leu Asn Asn Leu Lys Val Asn Gly Val Pro Ile Ser Val Ala 
545                 550                 555                 560 


Leu Lys Gln Lys Ile Tyr Glu Glu Leu Phe Lys Lys Gln Val Asn Val 
                565                 570                 575     


Thr Phe Asn Gln Leu Lys Thr Phe Leu Lys Thr Gln Gly Glu Asp Ala 
            580                 585                 590         


Asp Glu Ile Ile Leu Ser Gly Val Asp Glu Ala Ala His Lys Phe Thr 
        595                 600                 605             


Asn Asn Leu Gln Thr Tyr His Lys Phe Arg Ala Ile Leu Arg Asp Lys 
    610                 615                 620                 


Ile Glu Phe Glu Pro Gln Arg Ser Met Val Glu Asp Ile Ile Arg Trp 
625                 630                 635                 640 


Lys Cys Leu Met Gly Asp Asp Ile Thr Met Trp Lys His Lys Ile Glu 
                645                 650                 655     


Lys Glu Tyr Gly Asp Gln Leu Ser Glu Glu Gln Leu Lys Lys Ile Thr 
            660                 665                 670         


Arg Leu Asn Phe Asn Gly Trp Ser Arg Leu Ser Gln Glu Phe Leu Thr 
        675                 680                 685             


Asp Val Pro Gly Val Asp Ile Glu Ser Gly Glu Val Tyr Ser Gly Ile 
    690                 695                 700                 


Met Asp Ala Leu Trp Arg Thr Asn Glu Asn Ile Met Gln Leu Leu Ser 
705                 710                 715                 720 


Ser Arg Phe Thr Phe Ala Ala Glu Val Lys Lys Gln Asn Gln Gly Thr 
                725                 730                 735     


Gly Arg Ile Glu Lys Ile Thr Tyr Asp Glu Val Met Lys Asp Thr Tyr 
            740                 745                 750         


Leu Ser Pro Ala Val Lys Arg Ser Val Trp Gln Ala Ile Thr Leu Val 
        755                 760                 765             


Glu Glu Ile Arg Lys Ile Arg Lys Ala Asp Pro Lys Arg Ile Phe Val 
    770                 775                 780                 


Glu Met Thr Arg Gln Pro Asp Ala Lys Lys Glu Arg Lys Leu Ser Arg 
785                 790                 795                 800 


Arg Thr Gln Leu Leu Asn Ala Tyr Arg Ala Ile Gly Asn Asp Ile Ala 
                805                 810                 815     


Glu Tyr Glu Lys Glu His Val Ser Thr Leu Ile Thr Gln Leu Glu Ser 
            820                 825                 830         


Lys Lys Asp Glu Asp Leu Arg Arg Lys Lys Leu Tyr Leu Tyr Phe Thr 
        835                 840                 845             


Gln Met Gly Arg Cys Met Tyr Thr Gly Glu Arg Ile Asp Leu Ser Ser 
    850                 855                 860                 


Leu Leu Arg Asp Thr Asn Ser Asn Ile Tyr Asp Ile Asp His Ile Tyr 
865                 870                 875                 880 


Pro Arg Ser Leu Thr Lys Asp Asp Ser Leu Asp Asn Thr Val Leu Cys 
                885                 890                 895     


Lys Lys Glu Ile Asn Leu Lys Lys Gly Asn Lys Tyr Ile His Arg Val 
            900                 905                 910         


Phe Thr Asp Lys Glu Leu Lys Lys Gln Tyr Gly Tyr Trp Lys Ala Leu 
        915                 920                 925             


Lys Glu Lys Lys Leu Ile Ser Glu Arg Lys Phe Ser Arg Leu Met Arg 
    930                 935                 940                 


Thr Thr Asp Leu Thr Ala Asp Glu Leu Ala Gly Phe Ile Ser Arg Gln 
945                 950                 955                 960 


Leu Val Glu Thr Ser Gln Met Val Lys Ala Thr Ala Asp Ile Leu Glu 
                965                 970                 975     


Lys Ile Tyr Pro Asn Val Thr Ile Val Tyr Val Lys Ala Arg Thr Val 
            980                 985                 990         


Ser Asp Phe Arg His Asp Tyr Glu  Leu Pro Lys Val Arg  Ala Leu Asn 
        995                 1000                 1005             


Asp Cys  His His Ala His Asp  Ala Tyr Leu Asn Ile  Val Val Gly 
    1010                 1015                 1020             


Asn Val  Tyr Tyr Glu Lys Phe  Thr Lys Ser Pro Phe  Arg Phe Ile 
    1025                 1030                 1035             


Ala Asn  Gln Lys Gly Lys Gly  Asp Ile Lys Tyr Asn  Leu Tyr Arg 
    1040                 1045                 1050             


Leu Phe  Asp Lys Asp Val Lys  Val Gly Asp Arg Ile  Ile Trp Asn 
    1055                 1060                 1065             


Ala Lys  Glu Asp Leu Ala Arg  Val Lys Ala Thr Leu  Glu Arg Asp 
    1070                 1075                 1080             


Thr Val  Asn Val Val Arg Met  Pro Val Glu Gln Arg  Gly Gln Phe 
    1085                 1090                 1095             


Phe Asp  Ala Thr Ile Gln Lys  Lys Gly Thr Thr Lys  Asn Met Leu 
    1100                 1105                 1110             


Val Leu  Pro Leu Lys Gly Ser  Asp Glu Arg Leu Gln  Asp Thr Ser 
    1115                 1120                 1125             


Lys Tyr  Gly Tyr Tyr Val Arg  Pro Ser Gly Met Ser  Phe Met Val 
    1130                 1135                 1140             


Val Gln  His Glu Val Lys Gly  Lys Ser Arg Val Thr  Ile Glu Pro 
    1145                 1150                 1155             


Ala Leu  Leu Val Tyr Lys Asn  Lys Leu Lys Ser Glu  Asp Asp Tyr 
    1160                 1165                 1170             


Ala Lys  Tyr Cys Glu Asp Val  Leu Lys Leu Gln Lys  Pro Lys Val 
    1175                 1180                 1185             


Leu Phe  Lys Asn Ile Lys Phe  Gly Ser Lys Ile Leu  Phe Asp Gly 
    1190                 1195                 1200             


Tyr Pro  Phe Leu Leu Lys Gly  Arg Thr Glu Asn Arg  Ile Ile Met 
    1205                 1210                 1215             


Ala Asp  Ala Gln Ser Leu Phe  Leu Gly Asn Gln Glu  Leu Lys Ile 
    1220                 1225                 1230             


Leu Lys  Arg Val Thr Lys Ile  Ser Asp Asp Ile Asp  His Lys Val 
    1235                 1240                 1245             


Glu Ser  Arg Ile Lys Ile Thr  Asp Glu Glu Trp Thr  Thr Leu Trp 
    1250                 1255                 1260             


Lys Ser  Val Val Glu Gln Ile  Val Thr Gly Lys Phe  Arg Asn Arg 
    1265                 1270                 1275             


Leu Gly  Thr Leu Asn Gly Ile  Leu Gln Gly Arg Lys  Ile Lys Leu 
    1280                 1285                 1290             


Ser Lys  Asp Glu Glu Ala Arg  Leu Asn Glu Leu Asp  Ser Lys Asp 
    1295                 1300                 1305             


Gln Glu  Thr Phe Leu Ser Gln  Ile Leu Leu Glu Arg  Phe Leu Gln 
    1310                 1315                 1320             


Phe Ser  Thr Glu Glu Lys Thr  Arg Leu Leu Leu Gln  Val Ile Ser 
    1325                 1330                 1335             


Phe Ser  Gly Asn Thr Thr Gly  Val Asp Leu Arg Leu  Ile Gly Gly 
    1340                 1345                 1350             


Ser Ser  Gly Thr Gly Thr Ile  Lys Thr Asn Lys Asn  Ile Thr Asp 
    1355                 1360                 1365             


His Ser  Val Ile Leu Ile Asn  Glu Ser Pro Thr Gly  Leu Phe Thr 
    1370                 1375                 1380             


Asn Arg  Thr Val Leu Asn Gln  Pro 
    1385                 1390     


<210>  1140
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1140

Met Lys Ser Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Glu Ser Glu Asn Asp Asn Glu Lys Ser Glu Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Leu Val Pro Leu Thr Thr Asp Glu Arg Thr Asp 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Ser Lys 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Ala 
65                  70                  75                  80  


Leu Val Ser Glu Leu Lys Lys Glu His Trp Ile Asp Glu Gly Tyr Arg 
                85                  90                  95      


Phe Ser Glu Asp Gly Pro Gly Ser Thr Phe Ser Thr Leu Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Glu Glu Glu Ile Ser Leu Gln Asp Leu Ala Lys Val 
        115                 120                 125             


Leu Leu Gln Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Leu 
    130                 135                 140                 


Asn Ser Ala Glu Asp Gly Gln Ala Ile Asp Gly Met Ser Val Ala Lys 
145                 150                 155                 160 


Val Leu Tyr Asp Arg Gly Leu Thr Pro Gly Glu Tyr Val Tyr Gln Arg 
                165                 170                 175     


Met Leu Asp Gly Gln Tyr Ala Ile Pro Glu Phe Tyr Lys Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asp Lys Ile Trp Asn Leu Gln Lys Ser Tyr Tyr Pro 
        195                 200                 205             


Glu Leu Thr Ala Glu Leu Tyr Asp Lys Leu Thr Asp Lys Asn Lys Ser 
    210                 215                 220                 


Gln Thr Trp Ala Ile Cys Arg Asp Glu Leu Gly Val Gln Gly Phe Lys 
225                 230                 235                 240 


Ser Ser Phe Thr Gly Lys Glu Arg Leu Lys Glu Ala Tyr Phe Asn Arg 
                245                 250                 255     


Gln Gln Ala Ser Lys Gln Lys Val Asp Leu Glu Val Leu Thr Cys Val 
            260                 265                 270         


Leu Gln Asp Ile Asn Gly Gln Lys Ala Ser Leu Ser Gly Leu Leu Ser 
        275                 280                 285             


Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Arg Met Thr Val 
    290                 295                 300                 


Gly Gln Met Leu Val Arg Gln Ile Gln Glu Asp Pro Asn Arg Ser Leu 
305                 310                 315                 320 


Arg Asn Thr Val Phe Tyr Arg Gln Asp Tyr Ile Asp Glu Phe Glu Lys 
                325                 330                 335     


Ile Trp Ser Val Gln Ser Arg Phe His Pro Glu Leu Thr Gln Glu Arg 
            340                 345                 350         


Lys Lys Lys Leu Lys Asp Ile Ile Ile Phe Tyr Gln Arg Pro Leu Lys 
        355                 360                 365             


Ser Gln Lys Gly Leu Ile Ser Leu Cys Glu Phe Glu Ser Arg Glu Val 
    370                 375                 380                 


Asp Ile Ser Val Asp Gly Val Ile Lys Lys Lys Lys Ile Gly Pro Arg 
385                 390                 395                 400 


Val Cys Pro Arg Ser Ser Pro Leu Phe Gln Glu Phe Lys Val Tyr Gln 
                405                 410                 415     


Thr Val Asn Asn Ile Leu Val Asn Gly Glu Pro Leu Asp Asp Asn Asp 
            420                 425                 430         


Arg Glu Lys Leu Val Gly Glu Leu Ala Phe Arg Glu Lys Met Lys Gly 
        435                 440                 445             


Gln Asp Val Leu Lys Leu Leu Tyr Lys Thr Ser Lys Gly Met Arg Ile 
    450                 455                 460                 


Asn Phe Glu Glu Val Glu Gly Asn Arg Thr Met Ala Thr Ile Leu Lys 
465                 470                 475                 480 


Ala Cys Glu Lys Ile Val Glu Gly Leu Gly Tyr Asp Thr Arg Asp Leu 
                485                 490                 495     


Ser Lys Ile Thr Ala Ser Glu Lys Val Ser Arg Ile Glu Glu Ile Phe 
            500                 505                 510         


Ala Lys Glu Gly Phe Lys Ser Asp Phe Leu His Phe Asp Ser Ser Leu 
        515                 520                 525             


Pro Ser Pro Glu Phe Glu Ser Gln Ala Ser Tyr Ser Leu Trp His Leu 
    530                 535                 540                 


Leu Tyr Ser Tyr Glu Asn Asp Lys Ser Val Ser Gly Asn Glu Ser Leu 
545                 550                 555                 560 


Val Glu Lys Ile Lys Lys Leu Cys Ser Met Asp Glu Ala Ser Ala Lys 
                565                 570                 575     


Ile Leu Ser Gly Cys Thr Phe Leu Ser Asp Tyr Gly Ser Leu Ser Ala 
            580                 585                 590         


Arg Ala Met Arg Lys Ile Leu Pro Tyr Met Lys Asp Gly Leu Glu Tyr 
        595                 600                 605             


Ser Ala Ala Cys Ala Met Ala Gly Tyr Arg His Ser Lys Arg Ser Leu 
    610                 615                 620                 


Thr Lys Glu Glu Leu Asp Ser Arg Val Tyr Val Asp Arg Leu Glu Leu 
625                 630                 635                 640 


Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn 
                645                 650                 655     


Gln Met Ile Asn Val Val Asn Ser Val Val Asp Thr Tyr Gly Lys Pro 
            660                 665                 670         


Asp Glu Ile Arg Ile Glu Met Ala Arg Glu Leu Lys Lys Ser Ala Ser 
        675                 680                 685             


Glu Arg Glu Lys Ala Gln Lys Asp Ile Ala Ala Ala Ser Lys Val Asn 
    690                 695                 700                 


Glu Ser Ile Val Ala Thr Leu Lys Gly Ser Pro Phe Phe Ile Asp Ser 
705                 710                 715                 720 


Pro Ser Arg Asn Asp Ile Ile Arg Tyr Arg Leu Trp Glu Glu Leu Lys 
                725                 730                 735     


Pro Asn Gly Tyr Lys Thr Leu Tyr Ser Gly Thr Tyr Ile Pro Lys Glu 
            740                 745                 750         


Arg Leu Phe Ser Lys Asp Phe Asp Ile Glu His Ile Ile Pro Gln Ala 
        755                 760                 765             


Leu Leu Phe Asp Asp Ser Phe Ala Asn Lys Thr Ile Glu Ser Arg Gln 
    770                 775                 780                 


Cys Asn Ile Glu Lys Ser Asn Arg Thr Ala Met Asp Tyr Val Lys Ser 
785                 790                 795                 800 


Lys Tyr Asp Ser Phe Gly Val Gln Ala Tyr Ile Asp Arg Val Lys Gly 
                805                 810                 815     


Leu Thr Gly Val Ser Lys Thr Lys Ile Thr Arg Leu Leu Met Glu Ser 
            820                 825                 830         


Lys Asp Ile Pro Glu Asp Phe Ile Asn Arg Glu Leu Arg Asp Ser Gln 
        835                 840                 845             


Tyr Ile Ala Arg Lys Ala Arg Glu Ile Leu Glu Ser Met Val Lys Phe 
    850                 855                 860                 


Val Val Pro Thr Thr Gly Ser Val Thr Ala Arg Leu Arg Glu Asp Trp 
865                 870                 875                 880 


Gln Leu Val Asn Val Met Arg Glu Leu Asn Trp Asp Lys Tyr Glu Lys 
                885                 890                 895     


Leu Gly Leu Thr Ser Phe Arg Glu Asp Arg Asp Gly Arg Glu Ile Pro 
            900                 905                 910         


Val Ile Glu Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met 
        915                 920                 925             


Asp Ala Leu Thr Ile Ala Phe Thr Arg Arg Gln Phe Ile Gln Tyr Leu 
    930                 935                 940                 


Asn His Leu Asn Ala Lys Ile Asp Thr Val Asp Lys Thr Asp Ile Asp 
945                 950                 955                 960 


Leu Arg Asp Tyr Asn Leu Glu Asp Ile Ser Phe Asp Gly Leu Arg Asp 
                965                 970                 975     


Gly Asp Lys Phe Gly Ile Val Lys Ala Leu Gln Lys Lys Tyr Leu Tyr 
            980                 985                 990         


Lys Asp Asp Lys Gln Lys Tyr Arg  Phe Val Pro Pro Met  Pro Leu Asp 
        995                 1000                 1005             


Glu Phe  Arg Ile Glu Ala Lys  Arg Gln Ile Glu Asp  Val Leu Val 
    1010                 1015                 1020             


Ser Phe  Lys Ala Lys Asn Lys  Val Cys Thr Lys Asn  Val Asn Thr 
    1025                 1030                 1035             


Thr Lys  Val Arg Gly Gly Gln  Gln Arg Lys Ile Gln  Leu Thr Pro 
    1040                 1045                 1050             


Arg Gly  Pro Leu His Glu Asp  Thr Ile Tyr Gly Lys  Arg Leu Gln 
    1055                 1060                 1065             


Tyr Val  Thr Lys Glu Glu Lys  Val Gly Gly Ser Phe  Asp Glu Ala 
    1070                 1075                 1080             


Lys Ile  Ala Ser Val Cys Lys  Lys Ser Tyr Arg Glu  Ala Leu Met 
    1085                 1090                 1095             


Arg Arg  Leu Met Glu Cys Gly  Gly Asp Pro Lys Lys  Ala Phe Thr 
    1100                 1105                 1110             


Gly Lys  Asn Ser Leu Glu Lys  Asn Pro Val Trp Val  Asp Glu Phe 
    1115                 1120                 1125             


His Ser  Gly Glu Val Pro Leu  Arg Val Arg Thr Val  Ser Met Ala 
    1130                 1135                 1140             


Ser Val  Tyr Thr Val Arg Lys  Ser Val Gly Gln Ser  Leu Asn Ile 
    1145                 1150                 1155             


Glu Lys  Val Leu Asp Lys His  Ile Lys Glu Ile Leu  Arg Ala Arg 
    1160                 1165                 1170             


Leu Glu  Glu Tyr Gly Gly Asn  Ala Ser Lys Ala Phe  Ser Asn Leu 
    1175                 1180                 1185             


Asp Glu  Asn Pro Ile Trp Leu  Asn Lys Glu Lys Gly  Ile Ala Val 
    1190                 1195                 1200             


Lys Arg  Val Thr Ile Asp Ser  Gly Lys Asn Pro Ile  Pro Leu Arg 
    1205                 1210                 1215             


Ser Lys  Leu Asp Lys Asp Gly  Gln Ile Ile Tyr Gly  Glu Gly Gln 
    1220                 1225                 1230             


Asn Pro  Gln Glu Ser Asp Phe  Val Val Pro Gly Gly  Asn His His 
    1235                 1240                 1245             


Ala Ala  Ile Tyr Arg Thr Pro  Ser Gly Lys Leu Gln  Asp Val Val 
    1250                 1255                 1260             


Val Ser  Phe Tyr Glu Ala Val  Ser Arg Lys Asn Leu  Gly Leu Glu 
    1265                 1270                 1275             


Val Ile  Asp Arg Asn Phe Lys  Lys Asp Glu Gly Trp  Glu Phe Leu 
    1280                 1285                 1290             


Phe Thr  Leu Lys Gln Asn Glu  Tyr Phe Val Phe Pro  Asn Pro Ala 
    1295                 1300                 1305             


Thr Gly  Phe Ser Pro Glu Asp  Tyr Asp Leu Thr Asp  Pro Ala Asn 
    1310                 1315                 1320             


Asn Ala  Ile Ile Ser Pro Asn  Leu Tyr Arg Val Gln  Thr Val Ser 
    1325                 1330                 1335             


Val Gly  Asp Tyr Arg Phe Arg  His His Leu Glu Thr  Thr Val Glu 
    1340                 1345                 1350             


Asp Asn  Glu Lys Leu Arg Asp  Ile Ala Trp Lys Arg  Ile Gly Pro 
    1355                 1360                 1365             


Ser Gly  Leu Val Gly Ala Val  Lys Val Arg Ile Asp  Asn Leu Gly 
    1370                 1375                 1380             


Arg Ile  Leu Ala Val Gly Glu  Tyr Asp 
    1385                 1390         


<210>  1141
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1141

Met Glu Asn Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Phe Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Ala Thr Ala Glu Asp Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Leu Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Lys Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Ser Asp Glu Lys Ser Thr Ser Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Gly Phe Thr 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Ser His Gln Lys Asp Leu Ala Ala Leu 
                325                 330                 335     


Lys Gln Phe Ile Lys Asn Asn Leu Pro Lys Arg Tyr Asn Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Lys Ala Ser Ser Ala Glu Glu Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile His Tyr Leu His Asn Val Asp Gly Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             


Asp Pro Lys Asn Glu Glu Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 


Ser Ile Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Cys Asp 
            660                 665                 670         


Lys Gln Thr Gly Asp Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Lys 
        675                 680                 685             


Ile Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 


Lys Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asp Asp Val 
705                 710                 715                 720 


Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Tyr Ala Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ser Leu Lys Asn Leu Ala Pro Gly Leu Asp Ser Asn Ile Leu Lys 
785                 790                 795                 800 


Glu Asn Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Lys Pro Leu Asp 
            820                 825                 830         


Ile Asp Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val Val Gln 
865                 870                 875                 880 


Lys Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Glu Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp 
            900                 905                 910         


Glu Arg Asp Lys Val Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Ser Phe Asn Thr 
    930                 935                 940                 


Glu Val Asn Glu Lys Asn Gln Lys Ile Arg Thr Val Lys Ile Ile Thr 
945                 950                 955                 960 


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Ala Val Val Ala Lys Ala Ile  Leu Lys Lys Tyr Pro  Lys Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr Gln Lys Tyr Asp  Leu Lys Arg 
    1010                 1015                 1020             


Tyr Ile  Ser Arg Phe Lys Pro  Ser Lys Glu Ile Glu  Lys Ala Thr 
    1025                 1030                 1035             


Glu Lys  Tyr Phe Phe Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Glu 
    1040                 1045                 1050             


Glu Val  His Tyr Ala Asp Gly  Ile Ile Val Lys Arg  Glu Asn Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Lys Glu Lys 
    1070                 1075                 1080             


Asp Phe  Ala Thr Ile Lys Lys  Val Leu Ser Tyr Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Thr Glu Ile  Gln Thr His Gly Leu  Asp Arg Gly 
    1100                 1105                 1110             


Lys Pro  Lys Gly Leu Phe Asn  Ser Asn Pro Ser Pro  Lys Pro Ser 
    1115                 1120                 1125             


Glu Asp  Ser Lys Glu Asn Leu  Val Pro Ile Lys Gln  Gly Leu Asp 
    1130                 1135                 1140             


Pro Arg  Lys Tyr Gly Gly Tyr  Ala Gly Ile Ser Asn  Ser Tyr Ala 
    1145                 1150                 1155             


Val Leu  Val Lys Ala Ile Val  Glu Lys Gly Ala Lys  Lys Gln Gln 
    1160                 1165                 1170             


Lys Thr  Ile Leu Glu Phe Gln  Gly Ile Ser Ile Leu  Asp Lys Ile 
    1175                 1180                 1185             


Asn Phe  Glu Asn Asn Lys Glu  Asn Tyr Leu Leu Lys  Lys Arg Tyr 
    1190                 1195                 1200             


Ile Glu  Ile Leu Ser Thr Ile  Thr Leu Pro Lys Tyr  Ser Leu Phe 
    1205                 1210                 1215             


Glu Phe  Pro Asp Gly Thr Arg  Arg Arg Leu Ala Ser  Ile Leu Ser 
    1220                 1225                 1230             


Thr Asn  Asn Lys Arg Gly Glu  Ile His Lys Gly Asn  Glu Leu Val 
    1235                 1240                 1245             


Leu Pro  Gly Lys Tyr Thr Thr  Leu Leu Tyr His Ala  Lys Asn Ile 
    1250                 1255                 1260             


Asn Lys  Lys Leu Glu Pro Glu  His Leu Glu Tyr Val  Glu Lys His 
    1265                 1270                 1275             


Arg Asn  Asp Phe Ala Lys Leu  Leu Glu Cys Val Leu  Asn Phe Asn 
    1280                 1285                 1290             


Asp Lys  Tyr Val Gly Ala Leu  Lys Asn Gly Glu Arg  Ile Arg Gln 
    1295                 1300                 1305             


Ala Phe  Thr Asp Trp Glu Thr  Val Asp Ile Glu Lys  Leu Cys Phe 
    1310                 1315                 1320             


Ser Phe  Ile Gly Pro Glu Asn  Ser Lys Asn Ala Gly  Leu Phe Glu 
    1325                 1330                 1335             


Leu Thr  Ser Gln Gly Ser Ala  Ser Asp Phe Glu Phe  Leu Gly Val 
    1340                 1345                 1350             


Lys Ile  Pro Arg Tyr Arg Asp  Tyr Ala Pro Ser Ser  Leu Leu Lys 
    1355                 1360                 1365             


Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1370                 1375                 1380             


Ile Asp  Leu Ser Lys Leu Gly  Glu Asp 
    1385                 1390         


<210>  1142
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1142

Met Asn Asn Asn Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Ala Thr Ala Glu Asp Arg Arg 
    50                  55                  60                  


Phe Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Ser Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Ile Leu Lys Leu Phe Ala Tyr Glu Lys Ser Thr Asp Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Ser Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu 
                325                 330                 335     


Lys Gln Phe Ile Gln Asn Asn Leu Gln Glu Lys Tyr Asp Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Arg Tyr Ile Asn Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Ile Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Tyr Lys Glu Lys Ile Glu Thr Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asn Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Gln Ala Ser Ser Ala Glu Glu Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ser Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Gln Tyr Leu His Asn Val Asp Gly Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             


Asp Pro Lys Asn Glu Glu Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Ala 
625                 630                 635                 640 


Ser Ile Phe Asp Lys Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Cys Asp 
            660                 665                 670         


Lys Lys Thr Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Tyr 
        675                 680                 685             


Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 


Lys Asp Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asn Asp Val 
705                 710                 715                 720 


Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Leu Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Ala Pro Glu Ser Ile Val Ile Glu Ile Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ala Leu Lys Asn Leu Ala Pro Gly Leu Asp Ser Asn Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp 
            820                 825                 830         


Ile Asn Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Val Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val Val Gln 
865                 870                 875                 880 


Lys Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Glu His Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp 
            900                 905                 910         


Glu Arg Asp Lys Val Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr 
    930                 935                 940                 


Glu Val Asn Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr 
945                 950                 955                 960 


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Ala Val Val Ala Lys Ala Ile  Leu Lys Lys Tyr Pro  Lys Leu Glu 
        995                 1000                 1005             


Pro Gly  Phe Val Tyr Gly Asp  Tyr Gln Lys Tyr Asp  Ile Lys Arg 
    1010                 1015                 1020             


Tyr Ile  Ser Arg Ser Lys Asp  Pro Lys Glu Val Glu  Lys Ala Thr 
    1025                 1030                 1035             


Glu Lys  Tyr Phe Phe Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Glu 
    1040                 1045                 1050             


Glu Val  His Tyr Ala Asp Gly  Thr Ile Val Lys Arg  Glu Asn Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Lys Glu Lys 
    1070                 1075                 1080             


Asp Phe  Ala Thr Ile Lys Lys  Val Leu Ser Leu Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Thr Glu Ile  Gln Thr His Gly Leu  Asp Arg Gly 
    1100                 1105                 1110             


Lys Pro  Arg Gly Leu Phe Asn  Ser Asn Pro Ser Pro  Lys Pro Ser 
    1115                 1120                 1125             


Glu Asp  Arg Lys Glu Asn Leu  Val Pro Ile Lys Gln  Gly Leu Asp 
    1130                 1135                 1140             


Pro Arg  Lys Tyr Gly Gly Tyr  Ala Gly Ile Ser Asn  Ser Tyr Ala 
    1145                 1150                 1155             


Val Leu  Val Lys Ala Ile Ile  Glu Lys Gly Ala Lys  Lys Gln Gln 
    1160                 1165                 1170             


Lys Thr  Val Leu Glu Phe Gln  Gly Ile Ser Ile Leu  Asp Lys Ile 
    1175                 1180                 1185             


Asn Phe  Glu Lys Asn Lys Glu  Asn Tyr Leu Leu Glu  Lys Gly Tyr 
    1190                 1195                 1200             


Ile Lys  Ile Leu Ser Thr Ile  Thr Leu Pro Lys Tyr  Ser Leu Phe 
    1205                 1210                 1215             


Glu Phe  Pro Asp Gly Thr Arg  Arg Arg Leu Ala Ser  Ile Leu Ser 
    1220                 1225                 1230             


Thr Asn  Asn Lys Arg Gly Glu  Ile His Lys Gly Asn  Glu Leu Val 
    1235                 1240                 1245             


Ile Pro  Glu Lys Tyr Thr Thr  Leu Leu Tyr His Ala  Lys Asn Ile 
    1250                 1255                 1260             


Asn Lys  Thr Leu Glu Pro Glu  His Leu Glu Tyr Val  Glu Lys His 
    1265                 1270                 1275             


Arg Asn  Asp Phe Ala Lys Leu  Leu Glu Tyr Val Leu  Asn Phe Asn 
    1280                 1285                 1290             


Asp Lys  Tyr Val Gly Ala Leu  Lys Asn Gly Glu Arg  Ile Arg Gln 
    1295                 1300                 1305             


Ala Phe  Ile Asp Trp Glu Thr  Val Asp Ile Glu Lys  Leu Cys Phe 
    1310                 1315                 1320             


Ser Phe  Ile Gly Pro Arg Asn  Ser Lys Asn Ala Gly  Leu Phe Glu 
    1325                 1330                 1335             


Leu Thr  Ser Gln Gly Ser Ala  Ser Asp Phe Glu Phe  Leu Gly Val 
    1340                 1345                 1350             


Lys Ile  Pro Arg Tyr Arg Asp  Tyr Thr Pro Ser Ser  Leu Leu Asn 
    1355                 1360                 1365             


Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1370                 1375                 1380             


Ile Asp  Leu Ser Lys Leu Gly  Glu Asp 
    1385                 1390         


<210>  1143
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1143

Met Lys Asn Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser Val Gly 
1               5                   10                  15      


Phe Ala Ala Arg Asp Asp Glu Asn Asn Leu Ile Arg Val Lys Gly Lys 
            20                  25                  30          


Asn Val Ile Gly Ala Arg Leu Phe Ala Glu Gly Lys Thr Ala Gln Glu 
        35                  40                  45              


Arg Arg Met Ala Arg Thr Ser Arg Arg Arg Tyr Asn Arg Arg Lys Trp 
    50                  55                  60                  


Arg Leu Arg Leu Leu Asn Asp Ile Phe His Asp Glu Ile Val His Lys 
65                  70                  75                  80  


Asp Lys Asp Phe Phe Asn Arg Leu Lys Gln Ser Ser Ile Ser Pro Lys 
                85                  90                  95      


Asp Asp Arg Lys Lys Tyr Phe Gly Ser Leu Leu Phe Pro Glu Leu Ser 
            100                 105                 110         


Asp Ser Glu Phe Tyr His Asn Gly Asn Gln Thr Ile Tyr His Leu Arg 
        115                 120                 125             


His Arg Leu Met Thr Ser Asn Glu Lys Ala Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Leu Arg His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asp Asp 
145                 150                 155                 160 


Thr Pro Val Ser Ser Phe Glu Ala Ser Asp Leu Lys Leu Gly Ser Phe 
                165                 170                 175     


Ile Pro Thr Leu Asn Asp Leu Phe Glu Thr Ile Asp Val Asn Tyr Val 
            180                 185                 190         


Phe Asn Ser Glu Asn Thr Asn Gln Ile Glu Ser Ile Leu Leu Asn Asn 
        195                 200                 205             


Asp Leu Arg Asn Ser Asp Lys Ser Lys Gln Leu Val Asp Leu Leu Tyr 
    210                 215                 220                 


Arg Asn Val Ser Asp Val Asp Asp Lys Ser Leu Glu Lys Gln Phe Asn 
225                 230                 235                 240 


Lys Phe Arg Lys Asp Ile Ser Lys Glu Ile Ala Lys Ser Phe Val Gly 
                245                 250                 255     


Ser Lys Phe Asn Val Asn Lys Leu Leu Ala Tyr Glu Thr Asp Asp Gly 
            260                 265                 270         


Ser Lys Ile Ser Leu Ser Phe Ser Asp Ala Ser Ser Asp Asp Lys Met 
        275                 280                 285             


Leu Glu Leu Ala Asp Asn Leu Asp Asp Glu Arg Ile Asn Ile Leu Asn 
    290                 295                 300                 


Ala Leu Lys Val Ile Tyr Ser Arg Val Arg Leu Asn Gln Ile Ile Pro 
305                 310                 315                 320 


Asn Gly Met Gly Leu Ser Glu Ser Met Ile Ala Ser Tyr Glu Leu His 
                325                 330                 335     


Lys Lys Gln Leu Asn Asp Leu Lys Lys Leu Leu Asn Val Leu Pro Phe 
            340                 345                 350         


His Glu Lys Tyr Ala Val Lys Val Ala Tyr Ala Ala Tyr Ile Gly Asn 
        355                 360                 365             


Met Asp Ala Gln Lys Phe Thr Gln Ala Glu Leu Ser Thr Ile Glu Ser 
    370                 375                 380                 


Leu Lys Glu Lys Asp Ser Ile Lys Lys Val Ile Asp Cys Lys Thr Gly 
385                 390                 395                 400 


His Ile Thr Asn Glu Ser Leu Ile Ser Val Ile Asn Gln Ile Val Val 
                405                 410                 415     


Asn Pro Tyr Leu Glu Asn Ser Asp Phe Glu Leu Ile Asp Ile Val Lys 
            420                 425                 430         


Lys Ile Asp Gln Asp Ile Lys Asn Gly Glu Tyr Leu Leu Lys Gln Arg 
        435                 440                 445             


Asn Asn Gln Asn Gly Asn Ile Pro His Gln Leu His Gln Met Glu Met 
    450                 455                 460                 


Asp Gln Ile Ile Glu Lys Gln Ser Lys Tyr Tyr Pro Phe Leu Gly Glu 
465                 470                 475                 480 


Leu Asn Pro Asn Glu Asn Arg Arg His Ile Ala Lys Tyr Lys Ile Ser 
                485                 490                 495     


Glu Leu Val Ala Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr 
            500                 505                 510         


Pro Glu Glu Gln Ala Lys Thr Ser Gly Ala Ser Phe Ala Trp Met Lys 
        515                 520                 525             


Arg Lys Glu Asp Gly Glu Ile Thr Pro Trp Asn Phe Asp Gln Lys Val 
    530                 535                 540                 


Asp Arg Glu Ala Thr Ala Ser Arg Phe Ile Lys Arg Leu Thr Val Lys 
545                 550                 555                 560 


Asp Thr Tyr Leu Phe Asn Glu Asp Val Leu Pro Asp Asn Ser Leu Leu 
                565                 570                 575     


Tyr Gln Gln Phe Lys Val Leu Asn Glu Leu Asn Val Val Lys Val Asn 
            580                 585                 590         


Asn Lys Arg Leu Ser Gln Ala Glu Lys Gln Gly Ala Phe Asn Asp Leu 
        595                 600                 605             


Phe Lys Val Thr Lys Thr Val Thr Ala Lys Arg Leu Lys Asn Tyr Leu 
    610                 615                 620                 


Val Ser Asn Phe Asn His Leu Asp Thr Ile Glu Ile Thr Gly Leu Ser 
625                 630                 635                 640 


Asp Gly Glu Lys Phe Asn Asn Gly Leu Thr Ser Tyr Val Asp Phe Gln 
                645                 650                 655     


Lys Ile Phe Gly Asp Lys Ile Asn Asn Leu Glu Leu Met Asp Asp Phe 
            660                 665                 670         


Glu Ser Leu Ile Glu Trp Ser Thr Val Phe Glu Asp Arg His Ile Phe 
        675                 680                 685             


Glu Glu Lys Val Arg Asn Glu Ile Asn Trp Ile Ser Glu Ser Glu Leu 
    690                 695                 700                 


Lys Gln Leu Val Ala Lys Arg Tyr Ser Gly Trp Gly Arg Leu Ser Ser 
705                 710                 715                 720 


Lys Leu Leu Val Gly Ile Asn Asn Asp Glu Gly Arg Asn Ile Ile Glu 
                725                 730                 735     


Gln Leu Phe Glu Thr Ser Glu Thr Phe Met Glu Ala Ile Ser Arg Pro 
            740                 745                 750         


Glu Val Lys Lys Gln Ile Asp Glu Ile Asn Ser Asp Phe Ile Lys Asn 
        755                 760                 765             


Thr Asp Ile Glu Ser Val Leu Ala Asp Ala Tyr Thr Ser Pro Gln Asn 
    770                 775                 780                 


Lys Lys Ala Ile Arg Glu Thr Tyr Lys Ile Val Lys Asp Ile Gln Lys 
785                 790                 795                 800 


Ala Met Gly Gly Lys Ala Pro Glu Ser Ile Ser Ile Glu Phe Thr Arg 
                805                 810                 815     


Asn Pro Glu Asp Lys Gly Glu Leu Thr Arg Ser Arg His Ser Glu Ile 
            820                 825                 830         


Ser Arg Lys Tyr Lys Glu Leu Ser Lys Glu Ile Ser Lys Glu Leu Asn 
        835                 840                 845             


Asp Glu Leu Lys Ser Lys Lys Asn Ser Met Asp Ile Asp Lys Tyr Tyr 
    850                 855                 860                 


Leu Tyr Phe Met Gln Met Gly Lys Asp Leu Tyr Asp Gly Lys Ser Ile 
865                 870                 875                 880 


Asp Ile Asp Asp Leu Asn Asn Tyr Gln Ile Asp His Ile Val Pro Gln 
                885                 890                 895     


Ser Phe Tyr Lys Asp Asp Ser Phe Asp Asn Arg Val Leu Thr Tyr Tyr 
            900                 905                 910         


Thr Asn Asn Gln Lys Lys Gly Asn Asn Thr Pro Leu Asn Gly Leu Asn 
        915                 920                 925             


Ile Ser Asn Glu Thr Arg Lys Leu Trp Ser His Leu His Asp Leu Gly 
    930                 935                 940                 


Leu Ile Ser Lys Arg Lys Tyr Leu Asn Leu Thr Met Gln Gly Leu Asp 
945                 950                 955                 960 


Ser Leu Ser Lys Tyr Thr Lys His Gly Phe Ile Arg Arg Gln Leu Val 
                965                 970                 975     


Glu Thr Ser Gln Val Ile Lys Leu Val Ala Asn Ile Leu Ser Asp Glu 
            980                 985                 990         


Phe Ser Ala Asp Asn Thr Lys Ile  Ile Glu Val Lys Ala  Lys Met Asn 
        995                 1000                 1005             


Ser Gln  Met Arg His Ser Phe  Asn Leu Tyr Lys Ile  Arg Glu Leu 
    1010                 1015                 1020             


Asn Asp  Tyr His His Ala Leu  Asp Ala Tyr Leu Thr  Thr Phe Ile 
    1025                 1030                 1035             


Gly Gly  Tyr Leu Tyr Asn Arg  Tyr Pro Lys Leu Arg  Gly Met Phe 
    1040                 1045                 1050             


Val Tyr  Gly Asp Phe Lys Lys  Phe Asp Val Asn Asp  Ala Asn Asn 
    1055                 1060                 1065             


Val Lys  Thr Leu Asn Phe Leu  His Asp Ile Thr Asn  Pro Ser Asp 
    1070                 1075                 1080             


Lys Phe  Val Asn Gln Ile Tyr  Asp Glu Gln Ser Gly  Glu Leu Ile 
    1085                 1090                 1095             


Leu Asp  Arg Lys His Ala Ile  Glu Thr Ile Lys Lys  Ile Tyr Gln 
    1100                 1105                 1110             


Tyr Lys  Phe Met Leu Ile Thr  Arg Glu Val Ser Thr  Lys Asp Asp 
    1115                 1120                 1125             


Lys Leu  Ser Asp Gln Thr Val  Tyr Ser Ala Lys Arg  Ile Asn Asn 
    1130                 1135                 1140             


Val Ala  Ser Pro Ile Pro Val  Lys Lys Asp Ser Asn  Pro Glu Leu 
    1145                 1150                 1155             


Tyr Gly  Phe Arg Thr Gly Asn  Val Asp His His Met  Val Ile Val 
    1160                 1165                 1170             


Lys Tyr  Pro Lys Lys Asp Ser  Phe Val Tyr Lys Val  Val Gly Ile 
    1175                 1180                 1185             


Pro Ile  Arg Phe Met Gln Glu  Leu Arg Ser Ser Lys  Asn Gln Ser 
    1190                 1195                 1200             


Glu Tyr  Glu Asn Thr Leu Asn  Gln Ile Val Gln Ala  Lys Leu Asn 
    1205                 1210                 1215             


Arg Lys  Lys Phe Asp Phe Arg  Val Val Val Asp Asn  Val Met Tyr 
    1220                 1225                 1230             


Gly Gln  Leu Ile Gln Asp Gly  Gly Gln Lys Phe Thr  Leu Gly Ser 
    1235                 1240                 1245             


Ser Thr  Tyr Arg Tyr Asn Ala  Lys Gln Leu Val Leu  Asp Met Asp 
    1250                 1255                 1260             


Ser Leu  Arg Ile Leu Ser Asp  Glu Lys Tyr Arg Asn  Ser Leu Thr 
    1265                 1270                 1275             


Asp Asp  Glu Lys Asn Ser Glu  Phe Ile Asn Ile Tyr  Gln Gln Val 
    1280                 1285                 1290             


Leu Asn  Val Val Asn Glu Lys  Phe Asp Val Phe Asp  Val Arg Gly 
    1295                 1300                 1305             


Thr Arg  Asp Lys Leu Thr Lys  Gly Leu Asp Lys Phe  Leu Asn Ile 
    1310                 1315                 1320             


Thr Ser  Asn Asp Lys Glu Ile  Val Leu Leu Lys Ile  Leu Ser Gly 
    1325                 1330                 1335             


Leu His  Cys Asn Ala Ala Arg  Val Lys Ile Asn Glu  Ile Gly Ile 
    1340                 1345                 1350             


Asn Thr  Ser Phe Gly Glu Leu  Gln Thr Thr His Gly  Ile Val Leu 
    1355                 1360                 1365             


Ser Glu  Asn Ser Tyr Ile Ile  Tyr Gln Ser Pro Ser  Gly Leu Phe 
    1370                 1375                 1380             


Glu Arg  Lys Val Arg Leu Lys  Asp Leu 
    1385                 1390         


<210>  1144
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1144

Met Ala Asn Lys Thr Trp Tyr Leu Gly Leu Asp Ile Gly Ser Ala Ser 
1               5                   10                  15      


Val Gly Trp Ala Ala Thr Asp Thr Gly Tyr Lys Ile Ile Arg Lys Asn 
            20                  25                  30          


Lys Lys Arg Leu Trp Gly Val Arg Leu Phe Glu Glu Ala Lys Thr Ala 
        35                  40                  45              


Glu Asp Arg Arg Gly Tyr Arg Ser Ser Arg Arg Arg Leu Ala Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Asn Leu Leu Glu Glu Leu Phe Thr Ser Glu Val Lys 
65                  70                  75                  80  


Lys Val Asp Glu Asn Phe Phe Leu Arg Leu Lys Glu Ser Gln Tyr His 
                85                  90                  95      


Tyr Glu Asp Lys Thr His Lys Val Pro Tyr Ala Ile Phe Asn Asp Lys 
            100                 105                 110         


Asp Tyr Thr Asp Lys Asp Tyr Tyr Lys Asp Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Ser Lys Leu Met Thr Glu Glu Asn Pro Asp Ile Arg Lys Val 
    130                 135                 140                 


Phe Leu Ala Ile His His Ile Ile Lys Asn Arg Gly His Phe Leu Leu 
145                 150                 155                 160 


Gln Gly Gln Ser Phe Lys Asp Gly Asn Leu Asn Asn Leu Ile Lys Glu 
                165                 170                 175     


Leu Leu Glu Leu Asp Ile Leu His Val Gly Phe Glu Val Thr Glu Glu 
            180                 185                 190         


Val Val Asp Lys Ile Ala Asp Ile Ser Leu Glu Lys Lys Thr Ser Lys 
        195                 200                 205             


Asp Lys Leu Asn Asp Ile Lys Glu Leu Tyr Pro Lys Glu Lys Gln Leu 
    210                 215                 220                 


Leu Glu Val Phe Arg Leu Ile Phe Gly Gly Lys Thr Ser Leu Asp Lys 
225                 230                 235                 240 


Leu Phe Ala Ile Asp Glu Tyr Lys Glu Leu Asp Ala Ala Ile Lys Ser 
                245                 250                 255     


Val Ser Phe Lys Glu Lys Ile Tyr Glu Glu Val Arg His Asp Tyr Glu 
            260                 265                 270         


Gln Val Leu Ser Asn Tyr Ile Glu Leu Leu Asp Leu Ala Lys Leu Val 
        275                 280                 285             


Tyr Asp Ser Ile Ile Leu Ser Asp Ile Lys Lys Glu Gly Lys Thr Leu 
    290                 295                 300                 


Ser Glu Ser Lys Val Glu Leu Phe Glu Lys His Arg Asp Asp Leu Leu 
305                 310                 315                 320 


Lys Leu Lys Asn Leu Val Lys Asn Asp Ser Lys Leu Ser Glu Asp Lys 
                325                 330                 335     


Lys Val Glu Leu Tyr Ala Leu Ile Phe Lys Glu Asp Lys Asp Lys Gly 
            340                 345                 350         


Thr Asn Tyr Val Asn Tyr Ile Arg Lys Ser Glu Glu Gly Lys Gly Cys 
        355                 360                 365             


Asn Tyr Glu Asp Phe Lys Lys Phe Leu Val Lys Glu Leu Ala Lys Leu 
    370                 375                 380                 


Glu Glu Ser Ser Val Lys Thr Glu Ile Ile Lys Asp Leu Glu Leu Glu 
385                 390                 395                 400 


Gln Phe Leu Pro Leu Gln Arg Thr Lys Asp Asn Ser Val Val Pro Tyr 
                405                 410                 415     


Gln Ile His Lys Glu Glu Leu Val Lys Ile Leu Asp Asn Ala Ala Arg 
            420                 425                 430         


Tyr His Ser Phe Leu Asn Glu Lys Asp Glu Ser Gly Tyr Asn Val Arg 
        435                 440                 445             


Glu Lys Val Ile Gln Leu Leu Glu Phe Arg Ile Pro Tyr Tyr Val Gly 
    450                 455                 460                 


Pro Leu Asn Ser Ser Lys Lys Ala Lys Glu Gly Gly Phe Ala Trp Ser 
465                 470                 475                 480 


Val Arg Asn Thr Gly Tyr Glu Asn Thr Pro Val Thr Pro Trp Asn Tyr 
                485                 490                 495     


Ser Lys Val Ile Asp Glu Ser Ala Ser Ala Glu Lys Phe Ile Thr Asn 
            500                 505                 510         


Leu Thr Asn Lys Cys Thr Tyr Leu Lys Gly Glu Asp Val Leu Pro Lys 
        515                 520                 525             


Ser Ser Leu Leu Tyr Ser Glu Phe Ala Leu Leu Asn Glu Leu Asn Ala 
    530                 535                 540                 


Leu Lys Tyr Asp Gly Asn Arg Ile Ser Leu Glu Ala Arg Asn Thr Ile 
545                 550                 555                 560 


Ile Glu Lys Leu Phe Lys Gln Gln Gly Lys Lys Val Thr Lys Thr Ser 
                565                 570                 575     


Ile Lys Asn Leu Leu Lys Ala Glu Gly Tyr Ile Asp Gly Lys Gly Glu 
            580                 585                 590         


Ile Thr Gly Ile Asp Ile Thr Val Lys Asn Asp Leu Lys Ser Tyr Arg 
        595                 600                 605             


Asp Phe Lys Lys Ile Leu Gly Asn Lys Phe Asn Ser Glu His Val Glu 
    610                 615                 620                 


Asn Ile Ile Leu Trp Ile Thr Leu Tyr Gly Glu Ser Arg Lys Leu Ile 
625                 630                 635                 640 


Lys Ala Lys Ile Glu Ser Val Tyr Gly Glu Val Tyr Ser Lys Asp Glu 
                645                 650                 655     


Ile Thr Lys Met Ser Arg Leu Val Tyr Lys Asp Trp Gly Arg Phe Ser 
            660                 665                 670         


Arg Lys Leu Leu Thr Glu Leu Val Ser Lys Lys Leu Tyr Asn Glu Glu 
        675                 680                 685             


Thr Gly Glu Cys Leu Asn Ile Ile Gly Ala Met Arg Gln Asn Asn Ile 
    690                 695                 700                 


Leu Phe Met Glu Leu Leu Ala Asp Arg Phe Asp Tyr Ser Lys Gln Ile 
705                 710                 715                 720 


Val Glu Phe Asn Lys Glu Leu Gln Glu Asp Val Thr Glu Ile Ala Pro 
                725                 730                 735     


Glu Ile Leu Asp Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ser Ile 
            740                 745                 750         


Trp Gln Thr Val Arg Ile Val Glu Glu Leu Lys Lys Ile Ile Gly Cys 
        755                 760                 765             


Ala Pro Ala Lys Ile Phe Val Glu Thr Thr Arg Ser Asn Gln Glu Lys 
    770                 775                 780                 


Lys Lys Pro Thr Asp Ser Arg Lys Lys Gln Leu Glu Leu Ala Tyr Lys 
785                 790                 795                 800 


Ala Val Lys Lys Asp Val Lys Glu Leu Glu Lys Glu Ile Gly Thr Ile 
                805                 810                 815     


Asn Phe Asp Val Leu Asn Asp Arg Leu Ser Ser Val Glu Pro Ser Lys 
            820                 825                 830         


Leu Lys Ala Lys Lys Leu Tyr Leu Tyr Tyr Thr Gln Leu Gly Arg Cys 
        835                 840                 845             


Met Tyr Ser Glu Glu Pro Ile Asn Ile Ser Glu Leu Phe Asp Asn Asn 
    850                 855                 860                 


Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp 
865                 870                 875                 880 


Ser Phe Thr Asn Thr Val Leu Val Lys Arg Glu Ser Asn Ala Ala Lys 
                885                 890                 895     


Thr Asp Arg Tyr Pro Leu Gly Ser Asp Ile Gln Thr Pro Ser Asn Arg 
            900                 905                 910         


Arg Phe Trp Lys Phe Leu Lys Glu Lys Gly Leu Ile Ser Asp Glu Lys 
        915                 920                 925             


Tyr Asn Arg Leu Val Arg Thr Glu Glu Phe Ser Asp Glu Glu Leu Ser 
    930                 935                 940                 


Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Ser Gln Ser Val Lys Ala 
945                 950                 955                 960 


Val Ala Ser Ile Leu Gly Glu Leu Asn Pro Glu Thr Ser Ile Cys Tyr 
                965                 970                 975     


Ser Lys Ala Glu Asn Val Ser Ser Phe Arg Gln Asn Phe Gly Lys Ile 
            980                 985                 990         


Lys Lys Gly Asp Arg Asp Ser Lys  Asn Asn Asp Gln Leu  Ile Lys Val 
        995                 1000                 1005             


Arg Glu  Ile Asn Asp Tyr His  His Ala Lys Asp Ala  Tyr Leu Asn 
    1010                 1015                 1020             


Val Val  Val Gly Asn Val Tyr  Asp Thr Lys Phe Thr  Arg Asn Val 
    1025                 1030                 1035             


Phe Asn  Phe Ile Lys Asp Lys  Lys Asp Gly Arg Lys  Tyr Ser Leu 
    1040                 1045                 1050             


Asn Lys  Val Phe Tyr Glu Asn  Val Ser Asp Ser Lys  Thr Val Ala 
    1055                 1060                 1065             


Trp Glu  Met Asp Lys Thr Ile  His Gln Val Glu Lys  Val Met Asn 
    1070                 1075                 1080             


Asn Asn  Asn Ile Met Val Thr  Arg Arg Thr Ser Glu  Gln Lys Gly 
    1085                 1090                 1095             


Gly Leu  Phe Asp Ala Thr Val  Tyr Lys Ala Lys Val  Ala Ala Lys 
    1100                 1105                 1110             


Ala Lys  Asp Gly Val Tyr Tyr  Pro Leu Lys Thr Ser  Asn Ser Val 
    1115                 1120                 1125             


Val Lys  Asp Val Ala Lys Tyr  Gly Gly Tyr Thr Ser  Ile Gly Ile 
    1130                 1135                 1140             


Ala Tyr  Tyr Ser Ile Phe Glu  Tyr Thr Ile Val Asn  Lys Lys Gly 
    1145                 1150                 1155             


Glu Glu  Lys Ile Thr Arg Ile  Ile Pro Ile Pro Ile  Tyr Ile Ser 
    1160                 1165                 1170             


Gln Asn  Ile Lys Asp Ser Thr  Ala Leu Ile Glu Phe  Gly Lys Ser 
    1175                 1180                 1185             


Gln Ile  Phe Cys Arg Ala Tyr  Glu Lys Ile Lys Asp  Phe Lys Leu 
    1190                 1195                 1200             


Leu Tyr  Arg Lys Leu Cys Ile  Gly Ser Lys Ile Asn  Ile Asn Asn 
    1205                 1210                 1215             


Tyr Gln  Tyr Tyr Ile Gly Gly  Lys Ser Asp Glu Arg  Phe Tyr Tyr 
    1220                 1225                 1230             


Asp Ser  Ala Ile Gln Val Thr  Leu Asp Lys Lys Ser  Glu Lys Tyr 
    1235                 1240                 1245             


Leu Lys  Ile Leu Ser Lys Tyr  Gln Asn Trp Lys Lys  Glu Asn Lys 
    1250                 1255                 1260             


Asp Gly  Glu Leu Trp Glu Asn  Ile Thr Lys Glu Asn  Asn Ile Glu 
    1265                 1270                 1275             


Leu Tyr  Asn Lys Leu Val Glu  Lys Met Asn Ser Glu  Ile Phe Thr 
    1280                 1285                 1290             


Lys Lys  Lys Ser Asn Lys Tyr  Asn Glu Leu Asn Ser  Glu Glu Ile 
    1295                 1300                 1305             


Arg Asn  Asn Phe Ile Lys Ile  Ser Val Glu Glu Gln  Ala Lys Val 
    1310                 1315                 1320             


Leu Leu  Glu Ile Leu Asn Leu  Leu Thr Asn Lys Lys  Ser Ile Phe 
    1325                 1330                 1335             


Asp Leu  Lys Ser Ile Asp Ile  Lys Ala Ala Arg Gly  Lys Ile Ser 
    1340                 1345                 1350             


Phe Asn  Leu Thr Ser Leu Ile  Gln Phe Ser Ile Ile  Glu Gln Ser 
    1355                 1360                 1365             


Ile Thr  Gly Phe Tyr Glu Lys  Glu Ile Thr Ile Ile  Gly Asp Lys 
    1370                 1375                 1380             


Gly Asn  Asp Met Glu Asn Asn  Asn Ser 
    1385                 1390         


<210>  1145
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1145

Met Asn Asn Asn Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg 
    50                  55                  60                  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ser Pro Glu Ile Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Asn Phe Pro Thr Ile Tyr His Leu Arg Lys Gln Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Glu Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Asp Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Gly Phe Ala 
        275                 280                 285             


Glu Leu Phe Val Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu 
                325                 330                 335     


Lys Gln Phe Ile Gln Asn Asn Leu Gln Glu Lys Tyr Asp Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asn Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Lys Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Ile Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Gln Glu Asn Lys Glu Lys Ile Lys Lys Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Gly Asp Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Gln Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Thr Gln Tyr Leu His Asn Val Asp Gly Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Met Asp 
        595                 600                 605             


Asp Ala Glu Asn Glu Ala Thr Leu Glu Asn Ile Ile His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 


Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Ile Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Cys Asp 
            660                 665                 670         


Lys Lys Thr Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Tyr 
        675                 680                 685             


Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 


Lys Asp Ile Ile Gln Lys Ala Gln Val Val Gly Arg Thr Asn Asp Val 
705                 710                 715                 720 


Lys Gln Ile Val His Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Ile Met Gly 
            740                 745                 750         


His Thr Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ala Leu Lys Asn Leu Ala Pro Gly Leu Asp Ser Asn Ile Leu Lys 
785                 790                 795                 800 


Glu Tyr Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp 
            820                 825                 830         


Ile Asn Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Val Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val Val Gln 
865                 870                 875                 880 


Lys Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Glu Arg Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp 
            900                 905                 910         


Glu Arg Asp Lys Val Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr 
    930                 935                 940                 


Glu Val Thr Glu Lys Asp Lys Lys Asn Arg Asn Val Lys Ile Ile Thr 
945                 950                 955                 960 


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Ala Val Val Ala Lys Ala Ile  Leu Lys Lys Tyr Pro  Lys Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr Gln Lys Tyr Asp  Leu Lys Arg 
    1010                 1015                 1020             


Tyr Ile  Ser Arg Ser Lys Asp  Pro Lys Asp Val Glu  Lys Ala Thr 
    1025                 1030                 1035             


Glu Lys  Tyr Phe Phe Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Glu 
    1040                 1045                 1050             


Glu Val  His Tyr Ala Asp Gly  Thr Ile Val Lys Arg  Glu Asn Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Lys Glu Lys 
    1070                 1075                 1080             


Asp Phe  Ala Thr Ile Lys Lys  Val Leu Ser Leu Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Thr Glu Ile  Gln Thr His Gly Leu  Asp Arg Gly 
    1100                 1105                 1110             


Lys Pro  Arg Gly Leu Phe Asn  Ser Asn Pro Ser Pro  Lys Pro Ser 
    1115                 1120                 1125             


Glu Asp  Ser Lys Glu Asn Leu  Val Pro Ile Lys Gln  Gly Leu Asp 
    1130                 1135                 1140             


Pro Arg  Lys Tyr Gly Gly Tyr  Ala Gly Ile Ser Asn  Ser Tyr Ala 
    1145                 1150                 1155             


Val Leu  Val Lys Ala Ile Ile  Glu Lys Gly Ala Lys  Lys Gln Gln 
    1160                 1165                 1170             


Lys Thr  Val Leu Glu Phe Gln  Gly Ile Ser Ile Leu  Asp Lys Ile 
    1175                 1180                 1185             


Asn Phe  Glu Lys Asn Lys Glu  Asn Tyr Leu Leu Glu  Lys Gly Tyr 
    1190                 1195                 1200             


Ile Lys  Ile Leu Ser Thr Ile  Thr Leu Pro Lys Tyr  Ser Leu Phe 
    1205                 1210                 1215             


Glu Phe  Pro Asp Gly Thr Arg  Arg Arg Leu Ala Ser  Ile Leu Ser 
    1220                 1225                 1230             


Thr Asn  Asn Lys Arg Gly Glu  Ile His Lys Gly Asn  Glu Leu Val 
    1235                 1240                 1245             


Ile Ser  Glu Lys Tyr Thr Thr  Leu Leu Tyr His Ala  Lys Asn Ile 
    1250                 1255                 1260             


Asn Lys  Thr Leu Glu Pro Glu  His Leu Glu Tyr Val  Glu Lys His 
    1265                 1270                 1275             


Arg Asn  Asp Phe Ala Lys Leu  Leu Glu Ser Val Leu  Asp Phe Asn 
    1280                 1285                 1290             


Asp Lys  Tyr Val Gly Ala Leu  Lys Asn Gly Glu Arg  Ile Arg Gln 
    1295                 1300                 1305             


Ala Phe  Ile Asp Trp Glu Thr  Val Asp Ile Glu Lys  Leu Cys Phe 
    1310                 1315                 1320             


Ser Phe  Ile Gly Pro Arg Asn  Ser Lys Asn Ala Gly  Leu Phe Glu 
    1325                 1330                 1335             


Leu Thr  Ser Gln Gly Ser Ala  Ser Asp Phe Glu Phe  Leu Gly Val 
    1340                 1345                 1350             


Lys Ile  Pro Arg Tyr Arg Asp  Tyr Thr Pro Ser Ser  Leu Leu Asn 
    1355                 1360                 1365             


Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1370                 1375                 1380             


Ile Asp  Leu Ser Lys Leu Gly  Glu Asp 
    1385                 1390         


<210>  1146
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1146

Met Asn Asn Asn Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys 
            20                  25                  30          


Met Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu 
        35                  40                  45              


Ile Gly Ala Leu Leu Phe Asp Glu Gly Ala Thr Ala Glu Asp Arg Arg 
    50                  55                  60                  


Phe Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu 
65                  70                  75                  80  


Arg Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser 
                85                  90                  95      


Ser Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys 
            100                 105                 110         


Arg Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu 
        115                 120                 125             


Tyr His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala 
    130                 135                 140                 


Asp Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
145                 150                 155                 160 


His Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp 
                165                 170                 175     


Ile Lys Asn Asn Asp Ile Gln Lys Ile Phe Ser Glu Phe Ile Ser Ile 
            180                 185                 190         


Tyr Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln 
        195                 200                 205             


Val Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu 
    210                 215                 220                 


Arg Ile Leu Lys Leu Phe Ala Tyr Glu Lys Ser Thr Asp Leu Phe Ser 
225                 230                 235                 240 


Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His 
                245                 250                 255     


Phe Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr 
            260                 265                 270         


Asp Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala 
        275                 280                 285             


Asp Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser 
    290                 295                 300                 


Gly Ile Leu Thr Val Thr Asp Ser Ser Thr Lys Ala Pro Leu Ser Ala 
305                 310                 315                 320 


Ser Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu 
                325                 330                 335     


Lys Gln Phe Ile Gln Asn Asn Leu Gln Glu Lys Tyr Asp Glu Val Phe 
            340                 345                 350         


Ser Asp Gln Ser Lys Asp Gly Tyr Ala Arg Tyr Ile Asn Gly Lys Thr 
        355                 360                 365             


Thr Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe 
    370                 375                 380                 


Glu Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu 
385                 390                 395                 400 


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
                405                 410                 415     


Leu Gln Glu Met Asn Ala Ile Ile Arg Arg Gln Gly Glu His Tyr Pro 
            420                 425                 430         


Phe Leu Lys Glu Tyr Lys Glu Lys Ile Glu Thr Ile Leu Thr Phe Arg 
        435                 440                 445             


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asn Phe Ala 
    450                 455                 460                 


Trp Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu 
465                 470                 475                 480 


Glu Ile Val Asp Gln Ala Ser Ser Ala Glu Glu Phe Ile Asn Lys Met 
                485                 490                 495     


Thr Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His 
            500                 505                 510         


Ser Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val 
        515                 520                 525             


Lys Phe Ile Ser Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly 
    530                 535                 540                 


Gln Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val 
545                 550                 555                 560 


Thr Glu Lys Asp Ile Ile Gln Tyr Leu His Asn Val Asp Gly Tyr Asp 
                565                 570                 575     


Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             


Asp Pro Lys Asn Glu Glu Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 


Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Ala 
625                 630                 635                 640 


Ser Ile Phe Asp Lys Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     


Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Cys Asp 
            660                 665                 670         


Lys Lys Thr Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Tyr 
        675                 680                 685             


Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 


Lys Asp Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asn Asp Val 
705                 710                 715                 720 


Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Ser Ile Lys Leu Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Ala Pro Glu Ser Ile Val Ile Glu Ile Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu 
    770                 775                 780                 


Asp Ala Leu Lys Asn Leu Ala Pro Gly Leu Asp Ser Asn Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp 
            820                 825                 830         


Ile Asn Gln Leu Ser Ser Tyr Asp Ile Asp His Ile Val Pro Gln Ala 
        835                 840                 845             


Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys 
    850                 855                 860                 


Asp Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Leu Glu Val Val Gln 
865                 870                 875                 880 


Lys Arg Lys Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser 
                885                 890                 895     


Glu His Lys Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp 
            900                 905                 910         


Glu Arg Asp Lys Val Gly Phe Ile Arg Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr 
    930                 935                 940                 


Glu Val Asn Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr 
945                 950                 955                 960 


Leu Lys Ser Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr 
                965                 970                 975     


Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Ala Val Val Ala Lys Ala Ile  Leu Lys Lys Tyr Pro  Lys Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr Gln Lys Tyr Asp  Ile Lys Arg 
    1010                 1015                 1020             


Tyr Ile  Ser Arg Ser Lys Asp  Pro Lys Glu Val Glu  Lys Ala Thr 
    1025                 1030                 1035             


Glu Lys  Tyr Phe Phe Tyr Ser  Asn Leu Leu Asn Phe  Phe Lys Glu 
    1040                 1045                 1050             


Glu Val  His Tyr Ala Asp Gly  Thr Ile Val Lys Arg  Glu Asn Ile 
    1055                 1060                 1065             


Glu Tyr  Ser Lys Asp Thr Gly  Glu Ile Ala Trp Asn  Lys Glu Lys 
    1070                 1075                 1080             


Asp Phe  Ala Thr Ile Lys Lys  Val Leu Ser Leu Pro  Gln Val Asn 
    1085                 1090                 1095             


Ile Val  Lys Lys Thr Glu Ile  Gln Thr His Gly Leu  Asp Arg Gly 
    1100                 1105                 1110             


Lys Pro  Arg Gly Leu Phe Asn  Ser Asn Pro Ser Pro  Lys Pro Ser 
    1115                 1120                 1125             


Glu Asp  Arg Lys Glu Asn Leu  Val Pro Ile Lys Gln  Gly Leu Asp 
    1130                 1135                 1140             


Pro Arg  Lys Tyr Gly Gly Tyr  Ala Gly Ile Ser Asn  Ser Tyr Ala 
    1145                 1150                 1155             


Val Leu  Val Lys Ala Ile Ile  Glu Lys Gly Ala Lys  Lys Gln Gln 
    1160                 1165                 1170             


Lys Thr  Val Leu Glu Phe Gln  Gly Ile Ser Ile Leu  Asp Lys Ile 
    1175                 1180                 1185             


Asn Phe  Glu Lys Asn Lys Glu  Asn Tyr Leu Leu Glu  Lys Gly Tyr 
    1190                 1195                 1200             


Ile Lys  Ile Leu Ser Thr Ile  Thr Leu Pro Lys Tyr  Ser Leu Phe 
    1205                 1210                 1215             


Glu Phe  Pro Asp Gly Thr Arg  Arg Arg Leu Ala Ser  Ile Leu Ser 
    1220                 1225                 1230             


Thr Asn  Asn Lys Arg Gly Glu  Ile His Lys Gly Asn  Glu Leu Val 
    1235                 1240                 1245             


Ile Pro  Glu Lys Tyr Thr Thr  Leu Leu Tyr His Ala  Lys Asn Ile 
    1250                 1255                 1260             


Asn Lys  Thr Leu Glu Pro Glu  His Leu Glu Tyr Val  Glu Lys His 
    1265                 1270                 1275             


Arg Asn  Asp Phe Ala Lys Leu  Leu Glu Tyr Val Leu  Asn Phe Asn 
    1280                 1285                 1290             


Asp Lys  Tyr Val Gly Ala Leu  Lys Asn Gly Glu Arg  Ile Arg Gln 
    1295                 1300                 1305             


Ala Phe  Ile Asp Trp Glu Thr  Val Asp Ile Glu Lys  Leu Cys Phe 
    1310                 1315                 1320             


Ser Phe  Ile Gly Pro Arg Asn  Ser Lys Asn Ala Gly  Leu Phe Glu 
    1325                 1330                 1335             


Leu Thr  Ser Gln Gly Ser Ala  Ser Asp Phe Glu Phe  Leu Gly Val 
    1340                 1345                 1350             


Lys Ile  Pro Arg Tyr Arg Asp  Tyr Thr Pro Ser Ser  Leu Leu Asn 
    1355                 1360                 1365             


Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1370                 1375                 1380             


Ile Asp  Leu Ser Lys Leu Gly  Glu Asp 
    1385                 1390         


<210>  1147
<211>  1393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1147

Met Lys Arg Val Thr Glu Asp Tyr Ile Leu Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asn Ser Cys Gly Trp Ala Val Thr Asp Lys Lys Asn Asn Leu Leu Lys 
            20                  25                  30          


Leu Arg Gly Lys Thr Ala Ile Gly Ser His Leu Phe Glu Glu Gly His 
        35                  40                  45              


Thr Ala Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Arg Leu Leu Glu Glu Ile Phe Ala Glu Pro 
65                  70                  75                  80  


Met Ala Lys Val Asp Pro Gly Phe Phe Val Arg Leu His Gln Ser Trp 
                85                  90                  95      


Val Ser Pro Leu Asp Lys Ser Arg Lys Lys Tyr His Ala Ile Val Phe 
            100                 105                 110         


Pro Thr Ala Lys Glu Asp Lys Ala Phe Tyr Gln Lys Tyr Pro Thr Ile 
        115                 120                 125             


Tyr His Leu Arg Asp Ala Leu Met Thr Gln Asp Arg Gln Phe Asp Leu 
    130                 135                 140                 


Arg Glu Ile Phe Leu Ala Ile His His Ile Val Lys Phe Arg Gly Asn 
145                 150                 155                 160 


Phe Leu Gln Asp Thr Pro Val Lys Asp Phe Glu Ala Ser Lys Ile Glu 
                165                 170                 175     


Val Gly Pro Ile Leu Ser Lys Ile Asn Gly Ala Phe Ala Glu Lys Ile 
            180                 185                 190         


Val Glu Asp Gln Asp Pro Ile Glu Leu Asn Val Ala Asn Ala Ala Glu 
        195                 200                 205             


Ile Glu Ala Val Ile Arg Gly Lys Asp Ala Asp Lys Thr Leu Tyr Lys 
    210                 215                 220                 


Met Asp Lys Val Lys Lys Ile Ala Lys Leu Leu Thr Asp Ser Thr Ala 
225                 230                 235                 240 


Lys Glu Glu Lys Asn Val Ala Lys Gln Ile Ala Asn Ala Val Met Gly 
                245                 250                 255     


Tyr Lys Thr Gln Phe Glu Thr Ile Leu Gly Lys Glu Ile Asp Lys Ser 
            260                 265                 270         


Asp Lys Ala Gln Trp Glu Phe Lys Leu Thr Asp Gly Asp Ala Asp Asp 
        275                 280                 285             


Lys Leu Glu Ala Leu Leu Pro Asp Leu Asp Glu Asn Asp Gln Thr Ile 
    290                 295                 300                 


Ile Ala Glu Ile Lys Lys Leu Phe Ser Ala Ile Thr Leu Ser Thr Ile 
305                 310                 315                 320 


Val Asp Glu Asn Lys Ser Leu Ser Gln Ser Met Val Ala Lys Tyr Asn 
                325                 330                 335     


Lys His His Gln Asp Tyr Lys Leu Leu Lys Ala Tyr Ile Asp Thr Leu 
            340                 345                 350         


Gln Asp Arg Ser Ile Ala Lys Lys Leu Leu Leu Ala Tyr Asp Leu Tyr 
        355                 360                 365             


Val Asn Asn Arg His Gly Arg Leu Leu Glu Ala Lys Thr Glu Phe Lys 
    370                 375                 380                 


Gln Asp Gly Thr Leu Thr Lys Glu Asp Phe Tyr Lys Thr Val Lys Asp 
385                 390                 395                 400 


Asn Leu Asp Asp Ser Glu Leu Ala Arg Lys Ile Ala Gln Lys Ile Ala 
                405                 410                 415     


Ala Asp Asn Phe Met Pro Lys Gln Arg Thr Asn Ser Asn Gly Val Ile 
            420                 425                 430         


Pro Phe Gln Leu His Gln Ile Glu Leu Asp Lys Ile Ile Ala Asn Gln 
        435                 440                 445             


Ser Lys Tyr Tyr Pro Phe Leu Ala Ala Glu Asn Pro Val Glu Asp His 
    450                 455                 460                 


Arg Lys Gln Ala Pro Tyr Lys Leu Asp Glu Leu Val Arg Phe Arg Val 
465                 470                 475                 480 


Pro Tyr Tyr Val Gly Pro Met Ile Thr Pro Asp Glu Gln Glu Lys Thr 
                485                 490                 495     


Ser Gly Lys Ser Phe Ala Trp Met Val Arg Lys Glu Asp Gly Gln Ile 
            500                 505                 510         


Thr Pro Trp Asn Phe Glu Gln Lys Val Asp Arg Gln Glu Ser Ala Asn 
        515                 520                 525             


Lys Phe Ile Lys Arg Met Thr Ile Lys Asp Thr Tyr Leu Leu Gly Glu 
    530                 535                 540                 


Asp Val Leu Pro Ala Asn Ser Leu Leu Tyr Gln Arg Phe Glu Val Leu 
545                 550                 555                 560 


Asn Glu Leu Asn Asn Ile Arg Ile Asn Gly Ser Arg Ile Ser Ile Asp 
                565                 570                 575     


Leu Lys Gln Gln Ile Phe Asn Asp Leu Phe Glu Glu Lys Lys Thr Val 
            580                 585                 590         


Thr Glu Lys Ser Leu Thr Ser Tyr Leu Lys Gln Asn Leu His Leu Pro 
        595                 600                 605             


Thr Val Glu Ile Lys Gly Leu Ala Asp Pro Thr Lys Phe Asn Ser Ser 
    610                 615                 620                 


Leu Ala Ser Tyr Tyr His Leu Lys Ser Leu His Val Phe Asp Lys Glu 
625                 630                 635                 640 


Leu Ala Asp Pro Gln Tyr Gln Lys Asp Phe Glu Lys Ile Ile Glu Tyr 
                645                 650                 655     


Ser Ser Ile Phe Glu Asp Lys Lys Ile Tyr Gln Asp Lys Leu His Ala 
            660                 665                 670         


Glu Phe Lys Trp Leu Thr Pro Glu Gln Phe Lys Ala Ile Ser Thr Trp 
        675                 680                 685             


Arg Leu Gln Gly Trp Gly Arg Leu Ser Cys Arg Leu Leu Val Glu Leu 
    690                 695                 700                 


His Asp Thr Asn Gly Gln Asn Ile Met Glu Gln Leu Trp Asp Ser Gln 
705                 710                 715                 720 


Lys Asn Phe Met Gln Ile Val Thr Glu Pro Asp Phe Lys Asp Ala Ile 
                725                 730                 735     


Ala Lys Glu Asn Gln Asn Val Thr Gln Val Asn Gly Val Glu Glu Ile 
            740                 745                 750         


Leu Ala Asp Ala Tyr Thr Ser Pro Ala Asn Lys Lys Ala Ile Arg Gln 
        755                 760                 765             


Val Val Lys Val Val Ala Asp Val Val Lys Ala Ala Gly Gly Lys Lys 
    770                 775                 780                 


Pro Ala Gln Phe Ala Ile Glu Phe Thr Arg Asp Pro Asp Lys Asn Pro 
785                 790                 795                 800 


Gln Leu Ser His Ile Arg Gly Thr Lys Leu Leu Lys Ala Tyr Gln Glu 
                805                 810                 815     


Thr Ala Gly Glu Leu Val Asp Gln Lys Leu Thr Asp Ser Leu Lys Glu 
            820                 825                 830         


Ala Met Thr Ser Arg Lys Leu Leu Lys Asp Lys Tyr Phe Leu Tyr Phe 
        835                 840                 845             


Met Gln Thr Gly Arg Asp Ala Tyr Thr Gly Lys Lys Ile Asn Ile Asp 
    850                 855                 860                 


Glu Val Thr Thr Asn Tyr Gln Ile Asp His Ile Leu Pro Gln Ser Phe 
865                 870                 875                 880 


Ile Lys Asp Asp Ser Phe Asp Asn Arg Val Leu Thr Ala Thr Pro Leu 
                885                 890                 895     


Asn Ala Glu Lys Ser Asp Asp Val Pro Tyr Lys Arg Phe Ala Asn Asn 
            900                 905                 910         


Tyr Val Ser Asp Leu Lys Met Thr Val Gly Glu Met Trp Lys His Trp 
        915                 920                 925             


Gln Lys Ala Gly Ile Ile Asn Lys His Lys Leu Gly Asn Leu Leu Leu 
    930                 935                 940                 


Asp Pro Asp Arg Leu Asn Lys Phe Gln Lys Ser Gly Phe Ile Asn Arg 
945                 950                 955                 960 


Gln Leu Val Glu Thr Ser Gln Ile Val Lys Leu Val Ser Val Ile Leu 
                965                 970                 975     


Gln Asn Lys Tyr Pro Asp Ala Glu Ile Ile Thr Val Lys Ala Gly Asp 
            980                 985                 990         


Asn Ser Ala Leu Arg Gln Arg Leu  Asn Leu Tyr Lys Ser  Arg Asp Val 
        995                 1000                 1005             


Asn Asp  Tyr His His Ala Ile  Asp Ala Tyr Leu Ser  Ile Ile Cys 
    1010                 1015                 1020             


Gly Asn  Phe Leu Tyr Gln Val  Tyr Pro Lys Tyr Arg  Pro Tyr Phe 
    1025                 1030                 1035             


Val Tyr  Gly Lys Tyr Lys Lys  Phe Ser Gln Asp Pro  Asp Leu Gln 
    1040                 1045                 1050             


Lys Asp  Leu Ile Lys His Phe  Lys Gly Phe Thr Phe  Met Trp Pro 
    1055                 1060                 1065             


Leu Leu  Gln Lys Asp Asn Ser  Glu Arg Lys Ala Pro  Glu Glu Ile 
    1070                 1075                 1080             


Lys Glu  Asn Asn Ser Glu Arg  Ile Val Phe Tyr Lys  His Pro Asp 
    1085                 1090                 1095             


Ile Phe  Asp Lys Leu Arg Lys  Ala Tyr Asn Tyr Lys  Tyr Met Leu 
    1100                 1105                 1110             


Val Ser  Arg Glu Thr Thr Thr  Glu Ser Ser Ser Met  Phe Lys Met 
    1115                 1120                 1125             


Thr Ile  Phe Pro Arg Gly Glu  Arg Asp Arg Lys Lys  Thr Arg Lys 
    1130                 1135                 1140             


Leu Ile  Pro Lys Gly Asn Gly  Leu Asp Pro Lys Ile  Tyr Gly Gly 
    1145                 1150                 1155             


Tyr Ser  Gly Asn Thr Asp Ala  Tyr Met Val Ile Val  Lys Ile Asp 
    1160                 1165                 1170             


Lys Ala  Lys Glu Ser Ile Tyr  Lys Val Ile Gly Val  Pro Met Arg 
    1175                 1180                 1185             


Ala Leu  Thr Arg Leu Asn Gln  Ala Lys Lys Gln Gly  Asn Tyr Gln 
    1190                 1195                 1200             


Glu Glu  Leu His Gln Val Leu  Glu Pro Gln Ile Met  Leu Asp Lys 
    1205                 1210                 1215             


Asn Gly  Lys Pro Lys Arg Gly  Val Lys Gly Phe Arg  Val Ile Lys 
    1220                 1225                 1230             


Asp His  Val Pro Phe Lys Gln  Val Val Leu Asp Gly  Asp Lys Lys 
    1235                 1240                 1245             


Phe Met  Leu Tyr Ser Ala Gln  Tyr Lys Thr Asn Ala  Lys Gln Leu 
    1250                 1255                 1260             


Ala Leu  Ser Gln Glu Thr Met  Arg Ile Val Thr Asp  Asn Leu Lys 
    1265                 1270                 1275             


Lys Gly  Glu Asp Gln Asp Lys  Leu Leu Val Lys Ala  Tyr Asp Glu 
    1280                 1285                 1290             


Ile Leu  Gln Lys Val Asp Cys  Tyr Leu Ser Leu Phe  Asp Thr Ser 
    1295                 1300                 1305             


Asn Phe  Arg Asn Gly Leu His  Leu Gly Arg Asn Lys  Phe Ile Asp 
    1310                 1315                 1320             


Leu Glu  Val Asn Asp Lys Lys  Ile Thr Leu Ser Asn  Ile Leu Asn 
    1325                 1330                 1335             


Gly Leu  His Asp Asn Met Val  Leu Gly Asn Leu Lys  Asn Ile Gly 
    1340                 1345                 1350             


Ile Gly  Thr Pro Leu Gly Gln  Met Gln Glu Lys Ser  Gly Ile Thr 
    1355                 1360                 1365             


Leu Ser  Pro Glu Ala Val Leu  Ile Phe Gln Ser Pro  Thr Gly Leu 
    1370                 1375                 1380             


Phe Glu  Lys Arg Val Lys Ile  Ala Asp Leu 
    1385                 1390             


<210>  1148
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1148

Met Lys Lys Glu Ile Lys Asp Tyr Phe Leu Gly Leu Asp Val Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asp Thr Asp Tyr Lys Leu Leu Lys 
            20                  25                  30          


Ala Asn Arg Lys Asp Leu Trp Gly Met Arg Cys Phe Glu Thr Ala Glu 
        35                  40                  45              


Thr Ala Glu Val Arg Arg Leu His Arg Gly Ala Arg Arg Arg Ile Glu 
    50                  55                  60                  


Arg Arg Lys Lys Arg Ile Lys Leu Leu Gln Glu Leu Phe Ser Gln Glu 
65                  70                  75                  80  


Ile Ala Lys Ile Asp Glu Gly Phe Phe Gln Arg Met Lys Glu Ser Pro 
                85                  90                  95      


Phe Tyr Ala Glu Asp Lys Thr Ile Leu Gln Glu Asn Ala Leu Phe Asn 
            100                 105                 110         


Asp Lys Asp Phe Thr Asp Lys Thr Tyr His Lys Ala Tyr Pro Thr Ile 
        115                 120                 125             


Asn His Leu Ile Lys Ala Trp Ile Glu Asn Lys Val Lys Pro Asp Pro 
    130                 135                 140                 


Arg Leu Leu Tyr Leu Ala Cys His Asn Ile Ile Lys Lys Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Glu Gly Asp Phe Asp Ser Glu Asn Gln Phe Asp Thr Ser 
                165                 170                 175     


Ile Gln Ala Leu Phe Glu Tyr Leu Arg Glu Asp Met Glu Val Asp Ile 
            180                 185                 190         


Asp Ala Asp Ser Gln Lys Val Lys Glu Ile Leu Lys Asp Ser Ser Leu 
        195                 200                 205             


Lys Asn Ser Glu Lys Gln Ser Arg Leu Asn Lys Ile Leu Gly Leu Lys 
    210                 215                 220                 


Pro Ser Asp Lys Gln Lys Lys Ala Ile Thr Asn Leu Ile Ser Gly Asn 
225                 230                 235                 240 


Lys Ile Asn Phe Ala Asp Leu Tyr Asp Asn Pro Asp Leu Lys Asp Ala 
                245                 250                 255     


Glu Lys Asn Ser Ile Ser Phe Ser Lys Asp Asp Phe Asp Ala Leu Ser 
            260                 265                 270         


Asp Asp Leu Ala Ser Ile Leu Gly Asp Ser Phe Glu Leu Leu Leu Lys 
        275                 280                 285             


Ala Lys Ala Val Tyr Asn Cys Ser Val Leu Ser Lys Val Ile Gly Asp 
    290                 295                 300                 


Glu Gln Tyr Leu Ser Phe Ala Lys Val Lys Ile Tyr Glu Lys His Lys 
305                 310                 315                 320 


Thr Asp Leu Thr Lys Leu Lys Ala Val Ile Lys Lys His Phe Pro Asn 
                325                 330                 335     


Asn Tyr Lys Lys Val Phe Gly Tyr Asn Gln Asp Asn Glu Ser Lys Asn 
            340                 345                 350         


Asn Tyr Ser Gly Tyr Val Gly Val Cys Lys Thr Lys Asn Lys Lys Leu 
        355                 360                 365             


Val Ile Asn Asn Ser Val Asn Gln Glu Asp Phe Tyr Lys Phe Leu Lys 
    370                 375                 380                 


Pro Ile Leu Ser Ala Lys Ser Glu Ile Lys Glu Val Asn Asp Ile Leu 
385                 390                 395                 400 


Ala Glu Ile Glu Thr Gly Asn Phe Leu Pro Lys Gln Ile Ser Lys Ser 
                405                 410                 415     


Asn Ala Glu Ile Pro Tyr Gln Leu Arg Lys Met Glu Leu Glu Lys Ile 
            420                 425                 430         


Leu Ser Asn Ala Glu Lys His Phe Ser Phe Leu Lys Gln Lys Asp Glu 
        435                 440                 445             


Lys Gly Leu Ser His Ser Glu Lys Ile Ile Met Leu Leu Thr Phe Lys 
    450                 455                 460                 


Ile Pro Tyr Tyr Ile Gly Pro Ile Asn Asp Asn His Lys Lys Ser Phe 
465                 470                 475                 480 


Pro Asp Arg Cys Trp Val Val Lys Lys Glu Lys Ser Pro Ser Gly Lys 
                485                 490                 495     


Thr Thr Pro Trp Asn Phe Phe Asp His Ile Asp Lys Glu Lys Thr Ala 
            500                 505                 510         


Glu Glu Phe Ile Thr Ser Arg Thr Asn Phe Cys Thr Tyr Leu Ile Gly 
        515                 520                 525             


Glu Ser Val Leu Pro Lys Asn Ser Leu Leu Tyr Ser Glu Tyr Thr Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Leu Gln Ile Ile Ile Asp Gly Lys Asn Ile 
545                 550                 555                 560 


Cys Asp Thr Lys Leu Lys Gln Lys Ile Tyr Glu Asp Leu Phe Lys Lys 
                565                 570                 575     


Tyr Lys Lys Ile Thr Gln Lys Gln Ile Ser Thr Phe Ile Lys His Glu 
            580                 585                 590         


Gly Ile Cys Asn Lys Thr Asp Glu Val Ile Ile Leu Gly Ile Asp Lys 
        595                 600                 605             


Glu Cys Thr Ser Ser Leu Lys Ser Tyr Ile Glu Leu Lys Asn Ile Phe 
    610                 615                 620                 


Gly Lys Gln Val Asp Glu Ile Ser Thr Lys Asn Met Leu Glu Glu Ile 
625                 630                 635                 640 


Ile Arg Trp Ala Thr Ile Tyr Asp Glu Gly Glu Gly Lys Thr Ile Leu 
                645                 650                 655     


Lys Thr Lys Ile Lys Ala Glu Tyr Gly Lys Tyr Cys Ser Asp Glu Gln 
            660                 665                 670         


Ile Lys Lys Ile Leu Asn Leu Lys Phe Ser Gly Trp Gly Arg Leu Ser 
        675                 680                 685             


Arg Lys Phe Leu Glu Thr Val Thr Ser Glu Met Pro Gly Phe Ser Glu 
    690                 695                 700                 


Pro Val Asn Ile Ile Thr Ala Met Arg Glu Thr Gln Asn Asn Leu Met 
705                 710                 715                 720 


Glu Leu Leu Ser Ser Glu Phe Lys Phe Thr Glu Asn Ile Lys Lys Ile 
                725                 730                 735     


Asn Ser Gly Phe Glu Asp Ala Glu Lys Gln Phe Ser Tyr Asp Gly Leu 
            740                 745                 750         


Val Lys Pro Leu Phe Leu Ser Pro Ser Val Lys Lys Met Leu Trp Gln 
        755                 760                 765             


Thr Leu Lys Leu Val Lys Glu Ile Ser His Ile Thr Gln Ala Pro Pro 
    770                 775                 780                 


Lys Lys Ile Phe Ile Glu Met Ala Lys Gly Ala Glu Leu Glu Pro Ala 
785                 790                 795                 800 


Arg Thr Lys Thr Arg Leu Lys Ile Leu Gln Asp Leu Tyr Ser Ser Cys 
                805                 810                 815     


Lys Asn Asp Ala Asp Ala Phe Ser Ser Glu Ile Gln Tyr Leu Ser Gly 
            820                 825                 830         


Lys Ile Glu Asn Glu Asp Asn Leu Arg Leu Arg Ser Asp Lys Leu Tyr 
        835                 840                 845             


Leu Tyr Tyr Thr Gln Leu Gly Lys Cys Met Tyr Cys Gly Lys Pro Ile 
    850                 855                 860                 


Glu Ile Gly His Val Phe Asp Thr Ser Asn Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile Ser Asn Arg Val Leu 
                885                 890                 895     


Val Cys Ser Ser Cys Asn Lys Asn Lys Glu Asp Lys Tyr Pro Leu Lys 
            900                 905                 910         


Ser Glu Ile Gln Ser Lys Gln Arg Gly Phe Trp Asn Phe Leu Gln Arg 
        915                 920                 925             


Asn Asn Phe Ile Ser Leu Glu Lys Leu Asn Arg Leu Thr Arg Thr Thr 
    930                 935                 940                 


Pro Ile Ser Asp Asp Glu Thr Ala Lys Phe Ile Ala Arg Gln Leu Val 
945                 950                 955                 960 


Glu Thr Arg Gln Ala Thr Lys Val Ala Ala Lys Val Leu Glu Lys Met 
                965                 970                 975     


Phe Pro Glu Thr Lys Ile Val Tyr Ser Lys Ala Glu Thr Val Ser Met 
            980                 985                 990         


Phe Arg Asn Lys Phe Asp Ile Ile  Lys Cys Arg Glu Ile  Asn Asp Phe 
        995                 1000                 1005             


His His  Ala His Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val 
    1010                 1015                 1020             


Tyr Asn  Thr Lys Phe Thr Asn  Asn Pro Trp Asn Phe  Ile Lys Glu 
    1025                 1030                 1035             


Lys Arg  Asp Asn Pro Lys Ile  Ala Asp Thr Tyr Asn  Tyr Tyr Lys 
    1040                 1045                 1050             


Val Phe  Asp Tyr Asp Val Lys  Arg Asn Asn Ile Thr  Ala Trp Glu 
    1055                 1060                 1065             


Lys Gly  Lys Thr Ile Ile Thr  Val Lys Asp Met Leu  Lys Arg Asn 
    1070                 1075                 1080             


Thr Pro  Ile Tyr Thr Arg Gln  Ala Ala Cys Lys Lys  Gly Gly Leu 
    1085                 1090                 1095             


Phe Asp  Gln Thr Ile Met Lys  Lys Gly Leu Gly Gln  His Pro Leu 
    1100                 1105                 1110             


Lys Lys  Glu Gly Pro Phe Ser  Asn Ile Ser Lys Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Asn Lys  Val Ser Ala Ala Tyr  Tyr Thr Leu Ile Glu  Tyr Glu Glu 
    1130                 1135                 1140             


Lys Gly  Asn Lys Ile Arg Ser  Leu Glu Thr Ile Pro  Leu Tyr Leu 
    1145                 1150                 1155             


Val Lys  Asp Ile Gln Lys Asp  Gln Asp Val Leu Lys  Ser Tyr Leu 
    1160                 1165                 1170             


Thr Asp  Leu Leu Gly Lys Lys  Glu Phe Lys Ile Leu  Val Pro Lys 
    1175                 1180                 1185             


Ile Lys  Ile Asn Ser Leu Leu  Lys Ile Asn Gly Phe  Pro Cys His 
    1190                 1195                 1200             


Ile Thr  Gly Lys Thr Asn Asp  Ser Phe Leu Leu Arg  Pro Ala Val 
    1205                 1210                 1215             


Gln Phe  Cys Cys Ser Asn Asp  Glu Val Leu Tyr Phe  Lys Lys Ile 
    1220                 1225                 1230             


Ile Arg  Phe Ser Glu Ile Arg  Ser Gln Arg Glu Lys  Ile Gly Lys 
    1235                 1240                 1245             


Thr Ile  Ser Pro Tyr Glu Asp  Leu Ser Phe Arg Ser  Tyr Ile Lys 
    1250                 1255                 1260             


Glu Asn  Leu Cys Lys Lys Thr  Lys Asn Asp Glu Ile  Gly Glu Lys 
    1265                 1270                 1275             


Glu Phe  Tyr Asp Leu Leu Gln  Lys Lys Asn Leu Glu  Ile Tyr Asp 
    1280                 1285                 1290             


Met Leu  Leu Thr Lys His Lys  Asp Thr Ile Tyr Lys  Lys Arg Pro 
    1295                 1300                 1305             


Asn Ser  Ala Thr Ile Asp Ile  Leu Val Lys Gly Lys  Glu Lys Phe 
    1310                 1315                 1320             


Lys Ser  Leu Ile Ile Glu Asn  Gln Phe Glu Val Ile  Leu Glu Ile 
    1325                 1330                 1335             


Leu Lys  Leu Phe Ser Ala Thr  Arg Asn Val Ser Glu  Leu Gln His 
    1340                 1345                 1350             


Ile Gly  Gly Ser Lys Tyr Ser  Gly Val Ala Lys Ile  Gly Asn Lys 
    1355                 1360                 1365             


Ile Ser  Ser Leu Asp Asn Cys  Ile Leu Ile Tyr Gln  Ser Ile Thr 
    1370                 1375                 1380             


Gly Ile  Phe Glu Lys Arg Ile  Asp Leu Leu Lys Val  
    1385                 1390                 1395 


<210>  1149
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1149

Met Lys Lys Glu Ile Lys Asp Tyr Phe Leu Gly Leu Asp Val Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asp Thr Asp Tyr Lys Leu Leu Lys 
            20                  25                  30          


Ala Asn Arg Lys Asp Leu Trp Gly Met Arg Cys Phe Glu Thr Ala Glu 
        35                  40                  45              


Thr Ala Glu Val Arg Arg Leu His Arg Gly Ala Arg Arg Arg Ile Glu 
    50                  55                  60                  


Arg Arg Lys Lys Arg Ile Lys Leu Leu Gln Glu Leu Phe Ser Gln Glu 
65                  70                  75                  80  


Ile Ala Lys Ile Asp Glu Gly Phe Phe Gln Arg Met Lys Glu Ser Pro 
                85                  90                  95      


Phe Tyr Ala Glu Asp Lys Thr Ile Leu Gln Glu Asn Thr Leu Phe Asn 
            100                 105                 110         


Asp Lys Asp Phe Thr Asp Lys Thr Tyr His Lys Ala Tyr Pro Thr Ile 
        115                 120                 125             


Asn His Leu Ile Lys Ala Trp Ile Glu Asn Lys Val Lys Pro Asp Pro 
    130                 135                 140                 


Arg Leu Leu Tyr Leu Ala Cys His Asn Ile Ile Lys Lys Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Glu Gly Asp Phe Asp Ser Glu Asn Gln Phe Asp Thr Ser 
                165                 170                 175     


Ile Gln Ala Leu Phe Glu Tyr Leu Arg Glu Asp Met Glu Val Asp Ile 
            180                 185                 190         


Asp Ala Asp Ser Gln Lys Val Lys Glu Ile Leu Lys Asp Ser Ser Leu 
        195                 200                 205             


Lys Asn Ser Glu Lys Gln Ser Arg Leu Asn Lys Ile Leu Gly Leu Lys 
    210                 215                 220                 


Pro Ser Asp Lys Gln Lys Lys Ala Ile Thr Asn Leu Ile Ser Gly Asn 
225                 230                 235                 240 


Lys Ile Asn Phe Ala Asp Leu Tyr Asp Asn Pro Asp Leu Lys Asp Ala 
                245                 250                 255     


Glu Lys Asn Ser Ile Ser Phe Ser Lys Asp Asp Phe Asp Ala Leu Ser 
            260                 265                 270         


Asp Asp Leu Ala Ser Ile Leu Gly Asp Ser Phe Glu Leu Leu Leu Lys 
        275                 280                 285             


Ala Lys Ala Val Tyr Asn Cys Ser Val Leu Ser Lys Val Ile Gly Asp 
    290                 295                 300                 


Glu Gln Tyr Leu Ser Phe Ala Lys Val Lys Ile Tyr Glu Lys His Lys 
305                 310                 315                 320 


Thr Asp Leu Thr Lys Leu Lys Asn Val Ile Lys Lys His Phe Pro Lys 
                325                 330                 335     


Asp Tyr Lys Lys Val Phe Gly Tyr Asn Lys Asn Glu Lys Asn Asn Asn 
            340                 345                 350         


Asn Tyr Ser Gly Tyr Val Gly Val Cys Lys Thr Lys Ser Lys Lys Leu 
        355                 360                 365             


Ile Ile Asn Asn Ser Val Asn Gln Glu Asp Phe Tyr Lys Phe Leu Lys 
    370                 375                 380                 


Thr Ile Leu Ser Ala Lys Ser Glu Ile Lys Glu Val Asn Asp Ile Leu 
385                 390                 395                 400 


Thr Glu Ile Glu Thr Gly Thr Phe Leu Pro Lys Gln Ile Ser Lys Ser 
                405                 410                 415     


Asn Ala Glu Ile Pro Tyr Gln Leu Arg Lys Met Glu Leu Glu Lys Ile 
            420                 425                 430         


Leu Ser Asn Ala Glu Lys His Phe Ser Phe Leu Lys Gln Lys Asp Glu 
        435                 440                 445             


Lys Gly Leu Ser His Ser Glu Lys Ile Ile Met Leu Leu Thr Phe Lys 
    450                 455                 460                 


Ile Pro Tyr Tyr Ile Gly Pro Ile Asn Asp Asn His Lys Lys Ser Phe 
465                 470                 475                 480 


Pro Asp Arg Cys Trp Val Ile Lys Lys Glu Lys Ser Pro Ser Gly Lys 
                485                 490                 495     


Thr Thr Pro Trp Asn Phe Phe Asp His Ile Asp Lys Asp Lys Thr Ala 
            500                 505                 510         


Glu Ala Phe Ile Thr Ser Arg Thr Asn Phe Cys Thr Tyr Leu Val Gly 
        515                 520                 525             


Glu Ser Val Leu Pro Lys Ser Ser Leu Leu Tyr Ser Glu Tyr Thr Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Leu Gln Ile Ile Ile Asp Gly Lys Asn Ile 
545                 550                 555                 560 


Cys Asp Ile Lys Leu Lys Gln Lys Ile Tyr Glu Glu Leu Phe Lys Lys 
                565                 570                 575     


Tyr Lys Lys Ile Thr Gln Lys Gln Ile Ser Thr Phe Ile Lys His Glu 
            580                 585                 590         


Gly Ile Cys Asn Lys Thr Asp Glu Val Ile Ile Leu Gly Ile Asp Lys 
        595                 600                 605             


Glu Cys Thr Ser Ser Leu Lys Ser Tyr Ile Glu Leu Lys Asn Ile Phe 
    610                 615                 620                 


Gly Lys Gln Val Asp Glu Ile Ser Thr Lys Asn Met Leu Glu Glu Ile 
625                 630                 635                 640 


Ile Arg Trp Ala Thr Ile Tyr Asp Glu Gly Glu Gly Lys Thr Ile Leu 
                645                 650                 655     


Lys Thr Lys Ile Lys Ala Glu Tyr Gly Lys Tyr Cys Ser Asp Glu Gln 
            660                 665                 670         


Ile Lys Lys Ile Leu Asn Leu Lys Phe Ser Gly Trp Gly Arg Leu Ser 
        675                 680                 685             


Arg Lys Phe Leu Glu Thr Val Thr Ser Glu Met Pro Gly Phe Ser Glu 
    690                 695                 700                 


Pro Val Asn Ile Ile Thr Ala Met Arg Glu Thr Gln Asn Asn Leu Met 
705                 710                 715                 720 


Glu Leu Leu Ser Ser Glu Phe Lys Phe Thr Glu Asn Ile Lys Lys Ile 
                725                 730                 735     


Asn Ser Gly Phe Glu Asp Ala Glu Lys Gln Phe Ser Tyr Asn Gly Leu 
            740                 745                 750         


Val Lys Pro Leu Phe Leu Ser Pro Ser Val Lys Lys Met Leu Trp Gln 
        755                 760                 765             


Thr Leu Lys Leu Val Lys Glu Ile Ser His Ile Thr Gln Ala Pro Pro 
    770                 775                 780                 


Lys Lys Ile Phe Ile Glu Met Ala Lys Gly Ala Glu Leu Glu Pro Ala 
785                 790                 795                 800 


Arg Thr Lys Thr Arg Leu Lys Ile Leu Gln Asp Leu Tyr Asn Asn Cys 
                805                 810                 815     


Lys Asn Asp Ala Asp Ala Phe Ser Ser Glu Ile Lys Asp Leu Ser Gly 
            820                 825                 830         


Lys Ile Glu Asn Glu Asp Asn Leu Arg Leu Arg Ser Asp Lys Leu Tyr 
        835                 840                 845             


Leu Tyr Tyr Thr Gln Leu Gly Lys Cys Met Tyr Cys Gly Lys Pro Ile 
    850                 855                 860                 


Glu Ile Gly His Val Phe Asp Thr Ser Asn Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile Ser Asn Arg Val Leu 
                885                 890                 895     


Val Cys Ser Ser Cys Asn Lys Asn Lys Glu Asp Lys Tyr Pro Leu Lys 
            900                 905                 910         


Ser Glu Ile Gln Ser Lys Gln Arg Gly Phe Trp Asn Phe Leu Gln Arg 
        915                 920                 925             


Asn Asn Phe Ile Ser Leu Glu Lys Leu Asn Arg Leu Thr Arg Thr Thr 
    930                 935                 940                 


Pro Ile Ser Asp Asp Glu Thr Ala Lys Phe Ile Ala Arg Gln Leu Val 
945                 950                 955                 960 


Glu Thr Arg Gln Ala Thr Lys Val Ala Ala Lys Val Leu Glu Lys Met 
                965                 970                 975     


Phe Pro Glu Thr Lys Ile Val Tyr Ser Lys Ala Glu Thr Val Ser Met 
            980                 985                 990         


Phe Arg Asn Lys Phe Asp Ile Ile  Lys Cys Arg Glu Ile  Asn Asp Phe 
        995                 1000                 1005             


His His  Ala His Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val 
    1010                 1015                 1020             


Tyr Asn  Thr Lys Phe Thr Asn  Asn Pro Trp Asn Phe  Ile Lys Glu 
    1025                 1030                 1035             


Lys Arg  Asp Asn Pro Lys Ile  Ala Asp Thr Tyr Asn  Tyr Tyr Lys 
    1040                 1045                 1050             


Val Phe  Asp Tyr Asp Val Lys  Arg Asn Asn Ile Thr  Ala Trp Glu 
    1055                 1060                 1065             


Lys Gly  Lys Thr Ile Ile Thr  Val Lys Asp Met Leu  Lys Arg Asn 
    1070                 1075                 1080             


Thr Pro  Ile Tyr Thr Arg Gln  Ala Ala Cys Lys Lys  Gly Gly Leu 
    1085                 1090                 1095             


Phe Asp  Gln Thr Ile Met Lys  Lys Gly Leu Gly Gln  His Pro Leu 
    1100                 1105                 1110             


Lys Lys  Glu Gly Ala Phe Ser  Asn Ile Ser Lys Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Asn Lys  Val Ser Ala Ala Tyr  Tyr Thr Leu Ile Glu  Tyr Glu Glu 
    1130                 1135                 1140             


Lys Gly  Asn Lys Ile Arg Ser  Leu Glu Thr Ile Pro  Leu Tyr Leu 
    1145                 1150                 1155             


Val Lys  Asp Ile Gln Lys Asp  Gln Asp Val Leu Lys  Ser Tyr Leu 
    1160                 1165                 1170             


Thr Asp  Leu Leu Gly Lys Lys  Glu Ile Lys Ile Leu  Val Pro Lys 
    1175                 1180                 1185             


Ile Lys  Ile Asn Ser Leu Leu  Lys Ile Asn Gly Phe  Pro Cys His 
    1190                 1195                 1200             


Ile Thr  Gly Lys Thr Gly Asp  Ser Phe Val Leu Arg  Pro Ala Val 
    1205                 1210                 1215             


Gln Phe  Cys Cys Ser Asn Asp  Glu Val Leu Tyr Phe  Lys Lys Ile 
    1220                 1225                 1230             


Ile Arg  Phe Asn Glu Ile Arg  Ser Gln Arg Glu Lys  Met Gly Lys 
    1235                 1240                 1245             


Thr Ile  Ser Pro Tyr Glu Asp  Leu Ser Phe Arg Ser  Tyr Ile Lys 
    1250                 1255                 1260             


Glu Asn  Leu Cys Lys Lys Thr  Lys Ala Asp Glu Ile  Gly Glu Lys 
    1265                 1270                 1275             


Glu Phe  Tyr Asp Leu Leu Gln  Lys Lys Asn Leu Glu  Ile Tyr Asp 
    1280                 1285                 1290             


Met Leu  Leu Thr Lys His Lys  Asp Thr Ile Tyr Asn  Lys Arg Pro 
    1295                 1300                 1305             


Asn Ser  Thr Thr Leu Gly Met  Leu Thr Lys Gly Arg  Tyr Glu Phe 
    1310                 1315                 1320             


Ile Asn  Leu Lys Pro Lys Asp  Gln Ile Ile Val Met  Leu Glu Ile 
    1325                 1330                 1335             


Leu Lys  Leu Phe Cys Thr Thr  Arg Glu Ala Ile Asp  Leu Gly Leu 
    1340                 1345                 1350             


Ile Lys  Gly Lys Pro Ala Ala  Gly Val Ala Thr Leu  Gly Lys Lys 
    1355                 1360                 1365             


Ile Ser  Asn Pro Asp Asn Cys  Ile Leu Ile Tyr Gln  Ser Ile Thr 
    1370                 1375                 1380             


Gly Ile  Phe Glu Lys Arg Ile  Asp Leu Leu Lys Val  
    1385                 1390                 1395 


<210>  1150
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1150

Met Gly Asp Thr Val Lys Asn Tyr His Ile Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Ser Ser Val Gly Phe Ala Ala Arg Asp Asp Asp Asn Asn Leu Ile Arg 
            20                  25                  30          


Val Lys Gly Lys Asn Val Ile Gly Thr Arg Leu Phe Ser Glu Gly Gln 
        35                  40                  45              


Thr Ala Gln Glu Arg Arg Met Ala Arg Ala Ser Arg Arg Arg Tyr Asn 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Arg Leu Leu Asp Glu Ile Phe His Asp Glu 
65                  70                  75                  80  


Ile Ile His Lys Asp Ala Glu Phe Phe Asn Arg Leu Lys Gln Ser Ser 
                85                  90                  95      


Leu Ser Pro Lys Asp Glu Arg Lys Gln Tyr Phe Gly Ser Leu Leu Phe 
            100                 105                 110         


Pro Glu Val Gly Asp Ser Glu Tyr Tyr His Asp Gly Asn Gln Thr Ile 
        115                 120                 125             


Tyr His Leu Arg His Arg Leu Met Thr Gln Asp Glu Lys Ala Asp Ile 
    130                 135                 140                 


Arg Glu Ile Tyr Leu Ala Leu Arg His Ile Ile Lys Tyr Arg Gly Asn 
145                 150                 155                 160 


Phe Leu Asp Asp Thr Pro Val Ser Ser Phe Glu Ala Ser Asp Leu Lys 
                165                 170                 175     


Leu Ala Ser Phe Val Pro Thr Leu Asn Asp Leu Phe Glu Thr Ile Asp 
            180                 185                 190         


Val Asn Tyr Glu Phe Ser Met Asp Asn Ile Asn Glu Ile Glu Asp Ile 
        195                 200                 205             


Leu Leu Asn Asn Lys Leu Arg Asn Ser Asp Lys Ser Lys Gln Leu Pro 
    210                 215                 220                 


Asp Leu Leu Thr Glu Pro Ile Val Asn Ile Asp Asp Lys Ser Leu Glu 
225                 230                 235                 240 


Lys Gln Phe Asn Lys Glu Arg Thr Asp Ile Ser Lys Glu Ile Ala Lys 
                245                 250                 255     


Ser Val Val Gly Ser Lys Met Asn Val Asn Lys Leu Leu Ser Phe Glu 
            260                 265                 270         


Ser Ser Gly Gly Tyr Lys Ile Ala Phe Ser Phe Ser Asp Ala Ser Ser 
        275                 280                 285             


Glu Asp Lys Met Met Glu Leu Ser Asp Val Leu Asp Asp Ser Arg Met 
    290                 295                 300                 


Asn Ile Leu Asn Ile Leu Lys Val Ile Tyr Ser Arg Val Arg Leu Asn 
305                 310                 315                 320 


Gln Ile Ile Pro Ser Gly Met Gly Leu Ser Glu Ser Met Val Ala Ala 
                325                 330                 335     


Tyr Asp Leu His Lys Gln Gln Leu Lys Asp Leu Lys Lys Leu Leu Asn 
            340                 345                 350         


Val Leu Pro Phe Asn Glu Arg Tyr Ala Val Lys Ile Ala Tyr Ala Ala 
        355                 360                 365             


Tyr Ile Gly Asn Ile Asp Lys Gln Lys Phe Thr Lys Ser Glu Val Lys 
    370                 375                 380                 


Thr Ile Asp Lys Leu Lys Glu Lys Ser Ala Ile Met Asn Val Leu Asp 
385                 390                 395                 400 


Phe Lys Ser Gly His Ile Thr Asn Glu Glu Leu Ser Asn Val Ile Lys 
                405                 410                 415     


Val Val Ile Val Asp Lys Tyr Leu Asn Asp Glu Asn Phe Glu Tyr His 
            420                 425                 430         


Ser Ile Val Glu Lys Ile Asn Gln Asp Ile Ser Asn Gly Ser Tyr Leu 
        435                 440                 445             


Leu Lys Gln Arg Asn Asn Gln Asn Gly Asn Ile Pro His Gln Leu His 
    450                 455                 460                 


Gln Met Glu Leu Asp Gln Ile Ile Glu Asn Gln Ser Lys Tyr Tyr Pro 
465                 470                 475                 480 


Phe Leu Gly Glu Leu Asn Pro Asn Ser Asn Arg Asn His Ile Ala Lys 
                485                 490                 495     


Tyr Lys Ile Ser Glu Leu Val Ala Phe Arg Val Pro Tyr Tyr Val Gly 
            500                 505                 510         


Pro Leu Ile Thr Pro Glu Glu Gln Glu Lys Thr Ser Gly Ala Ser Phe 
        515                 520                 525             


Ala Trp Met Lys Arg Lys Glu Asp Gly Ala Ile Thr Pro Trp Asn Phe 
    530                 535                 540                 


Glu Glu Lys Val Asp Lys Glu Glu Thr Ala Ser Arg Phe Ile Lys Arg 
545                 550                 555                 560 


Leu Thr Val Lys Asp Thr Tyr Leu Leu Asn Glu Asp Val Leu Pro Asp 
                565                 570                 575     


Asn Ser Leu Leu Tyr Glu Lys Phe Lys Val Leu Asn Glu Leu Asn Val 
            580                 585                 590         


Val Lys Ala Asn Asn Lys Arg Leu Thr Val Glu Glu Lys Gln Gly Ala 
        595                 600                 605             


Phe Asp Asn Leu Phe Lys Lys Met Lys Thr Val Thr Ala Lys Lys Leu 
    610                 615                 620                 


Lys Asn Tyr Leu Val Ser Asn His Asn His Ile Asn Ser Val Glu Ile 
625                 630                 635                 640 


Thr Gly Leu Ser Asp Val Asp Lys Phe Asn Asn Ser Leu Ser Thr Tyr 
                645                 650                 655     


Ile Asp Phe Lys Lys Ile Phe Asp Asp Lys Ile Asp Asn Pro Asp Leu 
            660                 665                 670         


Leu Asp Asp Phe Glu Asp Ile Ile Glu Trp Ser Thr Val Phe Glu Asp 
        675                 680                 685             


Arg Lys Ile Phe Glu Glu Lys Val Arg Lys Asp Ile Ser Trp Ile Ser 
    690                 695                 700                 


Glu Ser Glu Ile Lys Ala Val Val Ser Lys Arg Tyr Ser Gly Trp Gly 
705                 710                 715                 720 


Arg Leu Ser Lys Lys Leu Leu Asn Gly Ile Thr Asn Asn Glu Gly Arg 
                725                 730                 735     


Asn Ile Ile Asp Glu Leu Trp Glu Glu Ser Glu Thr Phe Met Glu Ala 
            740                 745                 750         


Val Ser Lys Pro Glu Val Lys Glu Gln Ile Asp Gly Ile Asn Ser Asp 
        755                 760                 765             


Phe Ile Lys Asn Thr Asp Ile Glu Ser Val Leu Ala Asp Ala Tyr Thr 
    770                 775                 780                 


Ser Pro Gln Asn Lys Lys Ala Ile Arg Glu Thr His Lys Leu Val Lys 
785                 790                 795                 800 


Asp Ile Gln Lys Ala Met Gly Gly Gln Ala Pro Ala Ser Ile Ser Ile 
                805                 810                 815     


Glu Phe Thr Arg Asn Pro Gln Asp Lys Gly Gln Leu Thr Arg Ser Arg 
            820                 825                 830         


His Ser Glu Val Ser Arg Lys Tyr Lys Glu Ile Ser Lys Glu Val Ser 
        835                 840                 845             


Lys Thr Leu Asn Asp Glu Leu Lys Ser Lys Lys Asn Ser Met Asp Val 
    850                 855                 860                 


Asp Lys Tyr Tyr Leu Tyr Phe Met Gln Met Gly Ile Asp Leu Tyr Asp 
865                 870                 875                 880 


Gly Ser Ser Ile Asp Ile Asp Asp Leu Asn Asn Tyr Gln Ile Asp His 
                885                 890                 895     


Ile Ile Pro Gln Ser Phe Tyr Lys Asp Asp Ser Phe Asp Asn Arg Val 
            900                 905                 910         


Leu Thr His Tyr Ser Asn Asn Gln Lys Lys Gly Asn Asn Thr Pro Leu 
        915                 920                 925             


Asn Gly Leu Asn Ile Asp Asp Lys Thr Arg Ala Ile Trp Asn His Leu 
    930                 935                 940                 


Arg Asp Leu Gly Leu Ile Thr Lys Lys Lys Tyr Asn Gly Leu Thr Leu 
945                 950                 955                 960 


Lys Gly Met Asp Gln Leu Ser Lys Tyr Thr Lys His Gly Phe Ile Arg 
                965                 970                 975     


Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Val Ala Asn Ile 
            980                 985                 990         


Leu Ser Asp Glu Phe Ser Thr Asp  Asp Thr Lys Ile Ile  Glu Val Lys 
        995                 1000                 1005             


Ala Lys  Met Asn Ser Gln Ile  Arg Gln Val Phe Asn  Leu Tyr Lys 
    1010                 1015                 1020             


Val Arg  Glu Leu Asn Asp Tyr  His His Ala Val Asp  Ala Tyr Leu 
    1025                 1030                 1035             


Thr Thr  Phe Ile Gly Gly Tyr  Leu Tyr Asn Arg Tyr  Pro Asn Leu 
    1040                 1045                 1050             


Arg Asn  Met Phe Val Tyr Gly  Asp Phe Lys Lys Phe  Ser Ala Asp 
    1055                 1060                 1065             


Val Asp  Asn Leu Lys Thr Phe  Asn Phe Leu His Asp  Ile Thr Asn 
    1070                 1075                 1080             


Pro Ser  Asp Lys Phe Glu Asp  Arg Ile Phe Asp Glu  Lys Ser Gly 
    1085                 1090                 1095             


Glu Leu  Ile Leu Asp Arg Lys  His Ala Ile Glu Thr  Ile Lys Lys 
    1100                 1105                 1110             


Ile Tyr  Gln Tyr Lys Tyr Met  Leu Ile Thr Arg Glu  Val Ser Thr 
    1115                 1120                 1125             


Lys Asn  Gly Gln Leu Ser Asp  Gln Thr Val Tyr Ser  Ala Lys Arg 
    1130                 1135                 1140             


Ile Asn  Asn Val Lys Ala Pro  Ile Pro Val Lys Lys  Asp Ser Asn 
    1145                 1150                 1155             


Pro Glu  Leu Tyr Gly Phe Arg  Thr Gly Asn Val Asp  His His Met 
    1160                 1165                 1170             


Val Ile  Val Lys Tyr Pro Lys  Lys Asp Ser Tyr Asp  Tyr Lys Val 
    1175                 1180                 1185             


Val Gly  Ile Pro Leu Arg Phe  Val Gln Lys Leu Lys  Glu Thr Lys 
    1190                 1195                 1200             


Asp Asn  Lys Asp Tyr Glu Asn  Leu Leu Asn Gln Ile  Val Gln Met 
    1205                 1210                 1215             


Lys Leu  Asn Arg Lys Lys Gln  Asp Phe Ile Val Val  Val Asp Asn 
    1220                 1225                 1230             


Val Met  Tyr Gly Gln Leu Ile  Gln Asp Gly Gly Glu  Lys Tyr Thr 
    1235                 1240                 1245             


Leu Gly  Ser Ser Thr Tyr Lys  Tyr Asn Ala Lys Gln  Leu Val Ile 
    1250                 1255                 1260             


Asn Met  Asp Lys Thr Arg Ile  Leu Glu Asp Asn Arg  Tyr Leu Asn 
    1265                 1270                 1275             


Ser Leu  Asp Glu Gly Glu Val  Ser Glu Lys Leu Val  Asn Ile Tyr 
    1280                 1285                 1290             


Asn Asp  Ile Met Lys Val Val  Asn Ser Arg Phe Asp  Leu Phe Asp 
    1295                 1300                 1305             


Ile Asn  Lys Phe Arg Glu Lys  Leu Asn Asp Gly Leu  Asn Lys Phe 
    1310                 1315                 1320             


Lys Asn  Ile Ser Asn Glu Gln  Lys Glu Glu Thr Leu  Leu Lys Ile 
    1325                 1330                 1335             


Leu Ser  Gly Leu His Cys Gly  Ser Ala Asn Val Pro  Ile Lys Glu 
    1340                 1345                 1350             


Leu Gly  Leu Thr Thr Pro Leu  Gly Lys Leu Gln Leu  Pro Ser Gly 
    1355                 1360                 1365             


Ile Val  Leu Ser Glu Glu Ala  Val Val Ile Tyr Gln  Ser Pro Ser 
    1370                 1375                 1380             


Gly Leu  Phe Glu Asn Lys Val  Arg Leu Lys Asp Leu  
    1385                 1390                 1395 


<210>  1151
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1151

Met Lys Lys Glu Ile Lys Asp Tyr Phe Leu Gly Leu Asp Val Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asp Thr Asp Tyr Lys Leu Leu Lys 
            20                  25                  30          


Ala Asn Arg Lys Asp Leu Trp Gly Met Arg Cys Phe Glu Thr Ala Glu 
        35                  40                  45              


Thr Ala Glu Val Arg Arg Leu His Arg Gly Ala Arg Arg Arg Ile Glu 
    50                  55                  60                  


Arg Arg Lys Lys Arg Ile Lys Leu Leu Gln Glu Leu Phe Ser Gln Glu 
65                  70                  75                  80  


Ile Ala Lys Thr Asp Glu Gly Phe Phe Gln Arg Met Lys Glu Ser Pro 
                85                  90                  95      


Phe Tyr Ala Glu Asp Lys Thr Ile Leu Gln Glu Asn Thr Leu Phe Asn 
            100                 105                 110         


Asp Lys Asp Phe Ala Asp Lys Thr Tyr His Lys Ala Tyr Pro Thr Ile 
        115                 120                 125             


Asn His Leu Ile Lys Ala Trp Ile Glu Asn Lys Val Lys Pro Asp Pro 
    130                 135                 140                 


Arg Leu Leu Tyr Leu Ala Cys His Asn Ile Ile Lys Lys Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Glu Gly Asp Phe Asp Ser Glu Asn Gln Phe Asp Thr Ser 
                165                 170                 175     


Ile Gln Ala Leu Phe Glu Tyr Leu Arg Glu Asp Met Glu Val Asp Ile 
            180                 185                 190         


Asp Ala Asp Ser Gln Lys Val Lys Glu Ile Leu Lys Asp Ser Ser Leu 
        195                 200                 205             


Lys Asn Ser Glu Lys Gln Ser Arg Leu Asn Lys Ile Leu Gly Leu Lys 
    210                 215                 220                 


Pro Ser Asp Lys Gln Lys Lys Ala Ile Thr Asn Leu Ile Ser Gly Asn 
225                 230                 235                 240 


Lys Ile Asn Phe Ala Asp Leu Tyr Asp Asn Pro Asp Leu Lys Asp Ala 
                245                 250                 255     


Glu Lys Asn Ser Ile Ser Phe Ser Lys Asp Asp Phe Asp Ala Leu Ser 
            260                 265                 270         


Asp Asp Leu Ala Ser Ile Leu Gly Asp Ser Phe Glu Leu Leu Leu Lys 
        275                 280                 285             


Ala Lys Ala Val Tyr Asn Cys Ser Val Leu Ser Lys Val Ile Gly Asp 
    290                 295                 300                 


Glu Gln Tyr Leu Ser Phe Ala Lys Val Lys Ile Tyr Glu Lys His Lys 
305                 310                 315                 320 


Thr Asp Leu Thr Lys Leu Lys Asn Val Ile Lys Lys His Phe Pro Lys 
                325                 330                 335     


Asp Tyr Lys Lys Val Phe Gly Tyr Asn Lys Asn Glu Lys Asn Asn Asn 
            340                 345                 350         


Asn Tyr Ser Gly Tyr Val Gly Val Cys Lys Thr Lys Ser Lys Lys Leu 
        355                 360                 365             


Ile Ile Asn Asn Ser Val Asn Gln Glu Asp Phe Tyr Lys Phe Leu Lys 
    370                 375                 380                 


Thr Ile Leu Ser Ala Lys Ser Glu Ile Lys Glu Val Asn Asp Ile Leu 
385                 390                 395                 400 


Thr Glu Ile Glu Thr Gly Thr Phe Leu Pro Lys Gln Ile Ser Lys Ser 
                405                 410                 415     


Asn Ala Glu Ile Pro Tyr Gln Leu Arg Lys Met Glu Leu Glu Lys Ile 
            420                 425                 430         


Leu Ser Asn Ala Glu Lys His Phe Ser Phe Leu Lys Gln Lys Asp Glu 
        435                 440                 445             


Lys Gly Leu Ser His Ser Glu Lys Ile Ile Met Leu Leu Thr Phe Lys 
    450                 455                 460                 


Ile Pro Tyr Tyr Ile Gly Pro Ile Asn Asp Asn His Lys Lys Phe Phe 
465                 470                 475                 480 


Pro Asp Arg Cys Trp Val Val Lys Lys Glu Lys Ser Pro Ser Gly Lys 
                485                 490                 495     


Thr Thr Pro Trp Asn Phe Phe Asp His Ile Asp Lys Glu Lys Thr Ala 
            500                 505                 510         


Glu Ala Phe Ile Thr Ser Arg Thr Asn Phe Cys Thr Tyr Leu Val Gly 
        515                 520                 525             


Glu Ser Val Leu Pro Lys Ser Ser Leu Leu Tyr Ser Glu Tyr Thr Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Leu Gln Ile Ile Ile Asp Gly Lys Asn Ile 
545                 550                 555                 560 


Cys Asp Ile Lys Leu Lys Gln Lys Ile Tyr Glu Asp Leu Phe Lys Lys 
                565                 570                 575     


Tyr Lys Lys Ile Thr Gln Lys Gln Ile Ser Thr Phe Ile Lys His Glu 
            580                 585                 590         


Gly Ile Cys Asn Lys Thr Asp Glu Val Ile Ile Leu Gly Ile Asp Lys 
        595                 600                 605             


Glu Cys Thr Ser Ser Leu Lys Ser Tyr Ile Glu Leu Lys Asn Ile Phe 
    610                 615                 620                 


Gly Lys Gln Val Asp Glu Ile Ser Thr Lys Asn Met Leu Glu Glu Ile 
625                 630                 635                 640 


Ile Arg Trp Ala Thr Ile Tyr Asp Glu Gly Glu Gly Lys Thr Ile Leu 
                645                 650                 655     


Lys Thr Lys Ile Lys Ala Glu Tyr Gly Lys Tyr Cys Ser Asp Glu Gln 
            660                 665                 670         


Ile Lys Lys Ile Leu Asn Leu Lys Phe Ser Gly Trp Gly Arg Leu Ser 
        675                 680                 685             


Arg Lys Phe Leu Glu Thr Val Thr Ser Glu Met Pro Gly Phe Ser Glu 
    690                 695                 700                 


Pro Val Asn Ile Ile Thr Ala Met Arg Glu Thr Gln Asn Asn Leu Met 
705                 710                 715                 720 


Glu Leu Leu Ser Ser Glu Phe Thr Phe Thr Glu Asn Ile Lys Lys Ile 
                725                 730                 735     


Asn Ser Gly Phe Glu Asp Ala Glu Lys Gln Phe Ser Tyr Asp Gly Leu 
            740                 745                 750         


Val Lys Pro Leu Phe Leu Ser Pro Ser Val Lys Lys Met Leu Trp Gln 
        755                 760                 765             


Thr Leu Lys Leu Val Lys Glu Ile Ser His Ile Thr Gln Ala Pro Pro 
    770                 775                 780                 


Lys Lys Ile Phe Ile Glu Met Ala Lys Gly Ala Glu Leu Glu Pro Ala 
785                 790                 795                 800 


Arg Thr Lys Thr Arg Leu Lys Ile Leu Gln Asp Leu Tyr Asn Asn Cys 
                805                 810                 815     


Lys Asn Asp Ala Asp Ala Phe Ser Ser Glu Ile Lys Asp Leu Ser Gly 
            820                 825                 830         


Lys Ile Glu Asn Glu Asp Asn Leu Arg Leu Arg Ser Asp Lys Leu Tyr 
        835                 840                 845             


Leu Tyr Tyr Thr Gln Leu Gly Lys Cys Met Tyr Cys Gly Lys Pro Ile 
    850                 855                 860                 


Glu Ile Gly His Val Phe Asp Thr Ser Asn Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile Ser Asn Arg Val Leu 
                885                 890                 895     


Val Cys Ser Ser Cys Asn Lys Asn Lys Glu Asp Lys Tyr Pro Leu Lys 
            900                 905                 910         


Ser Glu Ile Gln Ser Lys Gln Arg Gly Phe Trp Asn Phe Leu Gln Arg 
        915                 920                 925             


Asn Asn Phe Ile Ser Leu Glu Lys Leu Asn Arg Leu Thr Arg Ala Thr 
    930                 935                 940                 


Pro Ile Ser Asp Asp Glu Thr Ala Lys Phe Ile Ala Arg Gln Leu Val 
945                 950                 955                 960 


Glu Thr Arg Gln Ala Thr Lys Val Ala Ala Lys Val Leu Glu Lys Met 
                965                 970                 975     


Phe Pro Glu Thr Lys Ile Val Tyr Ser Lys Ala Glu Thr Val Ser Met 
            980                 985                 990         


Phe Arg Asn Lys Phe Asp Ile Val  Lys Cys Arg Glu Ile  Asn Asp Phe 
        995                 1000                 1005             


His His  Ala His Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val 
    1010                 1015                 1020             


Tyr Asn  Thr Lys Phe Thr Asn  Asn Pro Trp Asn Phe  Ile Lys Glu 
    1025                 1030                 1035             


Lys Arg  Asp Asn Pro Lys Ile  Ala Asp Thr Tyr Asn  Tyr Tyr Lys 
    1040                 1045                 1050             


Val Phe  Asp Tyr Asp Val Lys  Arg Asn Asn Ile Thr  Ala Trp Glu 
    1055                 1060                 1065             


Lys Gly  Lys Thr Ile Ile Thr  Val Lys Asp Met Leu  Lys Arg Asn 
    1070                 1075                 1080             


Thr Pro  Ile Tyr Thr Arg Gln  Ala Ala Cys Lys Lys  Gly Glu Leu 
    1085                 1090                 1095             


Phe Asn  Gln Thr Ile Met Lys  Lys Gly Leu Gly Gln  His Pro Leu 
    1100                 1105                 1110             


Lys Lys  Glu Gly Pro Phe Ser  Asn Ile Ser Lys Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Asn Lys  Val Ser Ala Ala Tyr  Tyr Thr Leu Ile Glu  Tyr Glu Glu 
    1130                 1135                 1140             


Lys Gly  Asn Lys Ile Arg Ser  Leu Glu Thr Ile Pro  Leu Tyr Leu 
    1145                 1150                 1155             


Val Lys  Asp Ile Gln Lys Asp  Gln Asp Val Leu Lys  Ser Tyr Leu 
    1160                 1165                 1170             


Thr Asp  Leu Leu Gly Lys Lys  Glu Phe Lys Ile Leu  Val Pro Lys 
    1175                 1180                 1185             


Ile Lys  Ile Asn Ser Leu Leu  Lys Ile Asn Gly Phe  Pro Cys His 
    1190                 1195                 1200             


Ile Thr  Gly Lys Thr Asn Asp  Ser Phe Leu Leu Arg  Pro Ala Val 
    1205                 1210                 1215             


Gln Phe  Cys Cys Ser Asn Asn  Glu Val Leu Tyr Phe  Lys Lys Ile 
    1220                 1225                 1230             


Ile Arg  Phe Ser Glu Ile Arg  Ser Gln Arg Glu Lys  Ile Gly Lys 
    1235                 1240                 1245             


Thr Ile  Ser Pro Tyr Glu Asp  Leu Ser Phe Arg Ser  Tyr Ile Lys 
    1250                 1255                 1260             


Glu Asn  Leu Trp Lys Lys Thr  Lys Asn Asp Glu Ile  Gly Glu Lys 
    1265                 1270                 1275             


Glu Phe  Tyr Asp Leu Leu Gln  Lys Lys Asn Leu Glu  Ile Tyr Asp 
    1280                 1285                 1290             


Met Leu  Leu Thr Lys His Lys  Asp Thr Ile Tyr Lys  Lys Arg Pro 
    1295                 1300                 1305             


Asn Ser  Ala Thr Ile Asp Ile  Leu Val Lys Gly Lys  Glu Lys Phe 
    1310                 1315                 1320             


Lys Ser  Leu Ile Ile Glu Asn  Gln Phe Glu Val Ile  Leu Glu Ile 
    1325                 1330                 1335             


Leu Lys  Leu Phe Ser Ala Thr  Arg Asn Val Ser Asp  Leu Gln His 
    1340                 1345                 1350             


Ile Gly  Gly Ser Lys Tyr Ser  Gly Val Ala Lys Ile  Gly Asn Lys 
    1355                 1360                 1365             


Ile Ser  Ser Leu Asp Asn Cys  Ile Leu Ile Tyr Gln  Ser Ile Thr 
    1370                 1375                 1380             


Gly Ile  Phe Glu Lys Arg Ile  Asp Leu Leu Lys Val  
    1385                 1390                 1395 


<210>  1152
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1152

Met Asn Ala Asn Arg Glu Met Ala Tyr Tyr Ile Gly Ile Asp Met Gly 
1               5                   10                  15      


Thr Gly Ser Val Gly Met Ala Ile Thr Asp Glu Asn Tyr Asn Leu Leu 
            20                  25                  30          


Lys Val Lys Gly Gln Asp Tyr Trp Phe Val Arg Glu Phe Glu Thr Ala 
        35                  40                  45              


Asn Thr Gln Ile Lys Arg Arg Met His Arg Ile Ser Lys Arg Arg Leu 
    50                  55                  60                  


Arg Arg His Gln Val Arg Val Ala Leu Ile Arg Ser Tyr Phe Ala Glu 
65                  70                  75                  80  


Glu Ile Leu Lys Asn Asp Pro Leu Phe Phe Ile Arg Gln Asp Asn Ser 
                85                  90                  95      


Ile Tyr Tyr Leu Glu Asp Lys Asp Ser Val Leu Lys Thr Lys Asp Cys 
            100                 105                 110         


Leu Phe Ala Asp Tyr Asn Tyr His Asp Lys Asn Tyr Tyr Lys Asp Tyr 
        115                 120                 125             


Pro Thr Ile Phe His Leu Arg Gln Ala Phe Ile Lys Asp Thr Val Lys 
    130                 135                 140                 


Asp Asp Glu Asn His Thr Arg Tyr Ala Arg Leu Leu Tyr Leu Ala Ile 
145                 150                 155                 160 


Ile Asn Met Phe Glu His Arg Gly His Phe Leu Leu Asn Ser Val Ser 
                165                 170                 175     


Glu Asp Ile Asn Lys Glu Met Val Lys Asp Ala Gly Glu Gly Phe Thr 
            180                 185                 190         


Leu Tyr Leu Gln Glu Arg Ile Lys Glu Ile Lys Pro Val Ser Tyr Ser 
        195                 200                 205             


Asp Ile Leu Asp Val Leu Val Glu Lys Asn Ile Ser Arg Ser Glu Lys 
    210                 215                 220                 


Lys Glu Arg Ile Val Glu Leu Phe Gly Ile Lys Lys Ser Cys Lys Asn 
225                 230                 235                 240 


Gly Met Glu Ile Ile Lys Cys Leu Cys Gly Leu Glu Ala Asn Val Ala 
                245                 250                 255     


Ile Met Phe Asn Leu Asp Asp Ile Glu Asn Lys Leu Thr Ile Val Val 
            260                 265                 270         


Ser Lys Asp Asp Tyr Glu Asp Lys Leu Ala Glu Val Gln Pro Leu Val 
        275                 280                 285             


Asp Glu Glu Ser Phe Glu Leu Leu Ser Arg Ile Lys Gln Leu Tyr Asp 
    290                 295                 300                 


Tyr Ser Gln Leu Gln Ser Ile Leu Lys Gly Tyr Lys Glu Lys Glu Asp 
305                 310                 315                 320 


Gly Glu Asp Lys Tyr Leu Ser Asp Val Arg Val Glu Leu Tyr Glu Lys 
                325                 330                 335     


His Lys Ala Asp Leu Lys Leu Leu Lys Lys Val Tyr Lys Arg Asp Leu 
            340                 345                 350         


Asp Lys Lys Gly Lys Ile Cys Glu Gly Lys Asn Ile Gln Arg Ser Ser 
        355                 360                 365             


Lys Tyr Asn Phe Met Phe Arg Ser Gly Lys Asp Gly Thr Tyr Ser Ala 
    370                 375                 380                 


Tyr Thr Asn Ser Leu Asn Ser Phe Cys Ser Lys Thr Ile Glu Asn Lys 
385                 390                 395                 400 


Tyr Arg Arg Asn Met Lys Asn Arg Asp Arg Ala Ser Leu Tyr Asp Thr 
                405                 410                 415     


Ile Lys Lys Asp Leu Ser Gly Ile Glu Asn Asp Asp Val Lys Lys Ile 
            420                 425                 430         


Leu Arg Asp Ile Glu Thr Glu Lys Phe Leu Pro Lys Gln Ile Thr Gly 
        435                 440                 445             


Glu Asn Gly Val Ile Pro Asn Gln Ile His Ala Lys Glu Leu Lys Ala 
    450                 455                 460                 


Ile Leu Lys Asn Ala Glu Lys His Leu Pro Phe Leu Thr Glu Lys Asp 
465                 470                 475                 480 


Asp Ser Gly Leu Thr Val Ser Glu Arg Ile Ile Ser Leu Phe Arg Tyr 
                485                 490                 495     


Cys Ile Pro Tyr Tyr Ile Gly Pro Val Gly Lys Asp Ser Arg Thr Gly 
            500                 505                 510         


Trp Ala Ser Ile Lys Glu Glu Gly Gln Ile Phe Pro Trp Asn Ile Glu 
        515                 520                 525             


Gln Lys Ile Asp Val Asp Lys Thr Ser Glu Glu Phe Ile Thr Arg Leu 
    530                 535                 540                 


Ile Lys Thr Cys Thr Tyr Leu Ser Asn Glu Lys Val Val Pro Lys Gln 
545                 550                 555                 560 


Ser Leu Val Tyr Glu Arg Tyr Cys Val Leu Asn Glu Ile Asn Asn Ile 
                565                 570                 575     


Lys Ile Arg Gly Lys Arg Ile Asp Pro Gln Leu Lys Gln Glu Ile Phe 
            580                 585                 590         


Lys Glu Leu Tyr Ile Gly Ser Lys Cys Gly Lys Lys Val Thr Arg Lys 
        595                 600                 605             


Asn Leu Cys Gln Tyr Leu Val Asn Lys Gly Ala Ile Glu Glu Asp Ser 
    610                 615                 620                 


Glu Ile Ser Gly Ile Asp Lys Asp Leu Asn Thr Tyr Met Ser Ser Tyr 
625                 630                 635                 640 


Gly Lys Met Tyr Gln Ile Phe Gly Asp Lys Ile Lys Arg Asp Asp Ile 
                645                 650                 655     


Lys Glu Val Ala Glu Glu Ile Ile Tyr Tyr Gly Thr Ile Tyr Gly Gly 
            660                 665                 670         


Ala Lys Glu Met Phe Lys Lys Lys Ile Glu Gln Tyr Val Ser Glu Asp 
        675                 680                 685             


Ile Leu Asp Glu Ala Gln Met Asn Arg Ile Leu Gly Phe Lys Phe Lys 
    690                 695                 700                 


Asp Trp Ala Arg Leu Ser Lys Lys Phe Leu Tyr Leu Glu Gly Tyr Asp 
705                 710                 715                 720 


Lys Gly Asn Gln Asn Gln Leu Thr Thr Leu Met Gln Ala Leu Trp Asp 
                725                 730                 735     


Tyr Asn Leu Asn Thr Met Glu Leu Met Asn Ser Pro Met Phe Thr Phe 
            740                 745                 750         


Lys Glu Thr Leu Glu Lys Gln Lys Asn Lys Met Glu Lys Val Leu Cys 
        755                 760                 765             


Asp Phe Glu Tyr Glu Asp Leu Asp Glu Tyr Tyr Phe Ser Ala Pro Val 
    770                 775                 780                 


Lys Arg Met Val Trp Gln Thr Ile Ala Cys Leu Lys Glu Val Val Gln 
785                 790                 795                 800 


Ile Met Gly His Asn Pro Lys Arg Ile Phe Ile Glu Met Pro Arg Ala 
                805                 810                 815     


Asp Glu Glu Lys Gly Glu Gln Gly Arg Lys Ala Ser Arg Lys Asn Arg 
            820                 825                 830         


Leu Leu Ser Leu Tyr Glu Glu Ile Glu Asp Glu Arg Pro Trp Lys Lys 
        835                 840                 845             


Glu Ile Glu Asn Ala Ala Glu Ser Gly Arg Leu Asn Ser Lys Arg Met 
    850                 855                 860                 


Tyr Leu Tyr Tyr Leu Gln Met Gly Lys Asp Ala Tyr Thr Gly Glu Asp 
865                 870                 875                 880 


Ile Asp Ile Glu Asp Leu Phe Asp Asn Ser Lys Tyr Asp Ile Asp His 
                885                 890                 895     


Ile Tyr Pro Arg Ser Leu Thr Asp Asp Asn Ser Leu Glu Asn Asn Leu 
            900                 905                 910         


Val Leu Val Ser Ala Thr Ile Asn Gln Asp Val Lys Lys Asn Asp Tyr 
        915                 920                 925             


Pro Ile Pro Tyr Asp Lys Ile Arg Gly Asn Ser Lys Val Arg Glu Leu 
    930                 935                 940                 


Trp Arg Phe Leu His Glu Lys Lys Phe Met Asn Asp Glu Lys Tyr Asn 
945                 950                 955                 960 


Arg Leu Met Ser Asp Val Pro Leu Thr Gln Glu Gln Leu Ala Gly Tyr 
                965                 970                 975     


Ile Ala Arg Gln Leu Val Glu Thr Ala Gln Gly Thr Lys Gly Ile Ala 
            980                 985                 990         


Asp Ile Ile Lys Ser Val Ile Pro  Asp Ala Asp Ile Ile  Tyr Ser Lys 
        995                 1000                 1005             


Ala Lys  Asn Val Ser Asp Phe  Arg Lys Arg Tyr Gly  Met Leu Lys 
    1010                 1015                 1020             


Ser Arg  Leu Thr Asn Asp Phe  His His Ala Lys Asp  Ala Tyr Leu 
    1025                 1030                 1035             


Asn Val  Val Val Gly Asn Val  Tyr Phe Thr Lys Phe  Thr Gln Asn 
    1040                 1045                 1050             


Pro Arg  Lys Tyr Ile Lys Asp  Glu Asn Asp Ile Glu  Asn Lys Lys 
    1055                 1060                 1065             


Tyr Asn  Tyr Asn Leu Ser Lys  Met Tyr Asp Lys Asp  Val Ile Arg 
    1070                 1075                 1080             


Asn Gly  Lys Val Ala Trp Ile  Ala Gln Asn Gly Lys  Lys Ala Gly 
    1085                 1090                 1095             


Thr Ile  Gln Thr Ile Lys Lys  Val Met Asp Arg Asn  Thr Pro Ile 
    1100                 1105                 1110             


Ile Thr  Arg Gln Ala Phe Glu  Gln Arg Gly Glu Leu  Tyr Asp Leu 
    1115                 1120                 1125             


Gln Pro  Ile Ser Lys His Lys  Ala Lys Lys Glu Asn  Tyr Val Pro 
    1130                 1135                 1140             


Ile Lys  Ala Thr Asn Glu Lys  Met Lys Asp Val Thr  Lys Tyr Gly 
    1145                 1150                 1155             


Gly Phe  Thr Ser Leu Asn Pro  Ala Tyr Phe Val Phe  Val Glu Tyr 
    1160                 1165                 1170             


Gly Ser  Ala Lys Lys Arg Gln  Arg Cys Phe Ala Val  Val His Ser 
    1175                 1180                 1185             


Tyr Trp  Ala Ser Ser Ile Lys  Asn Asn Glu Asp Leu  Val Thr His 
    1190                 1195                 1200             


Leu Lys  Asp Arg Gly Tyr Glu  Asn Pro Ile Val Ile  Ala Asp Lys 
    1205                 1210                 1215             


Ile Lys  Lys Asn Ser Leu Ile  Lys Tyr Asn Gly Tyr  Phe Leu Tyr 
    1220                 1225                 1230             


Ile Val  Gly Met Asp Ala Arg  Lys Gln Val Glu Phe  Ser Asn Ala 
    1235                 1240                 1245             


Thr Ser  Met Cys Leu Asn Asn  Asn Phe Ile Gln Tyr  Ile Gln Lys 
    1250                 1255                 1260             


Ile Glu  Lys Ala Asn Thr Leu  Ile Ala Glu Ala Lys  Lys Arg Arg 
    1265                 1270                 1275             


Pro Tyr  Ile Asp Glu Ser Ile  Thr Ala Glu Arg Asn  Ile Ala Leu 
    1280                 1285                 1290             


Tyr Asn  Glu Leu Lys Lys Lys  His Leu Asp Ser Ile  Phe Lys Asn 
    1295                 1300                 1305             


His Pro  Lys Gly Ile Gly Ser  Val Leu Glu Ser Gly  Glu Gly Ala 
    1310                 1315                 1320             


Phe Val  Gln Leu Ser Leu Glu  Gln Gln Ile Gly Ile  Leu Tyr Ser 
    1325                 1330                 1335             


Ile Ile  Gln Phe Ser Ser Phe  Lys Lys Gly Thr Phe  Ser Leu Thr 
    1340                 1345                 1350             


Leu Ile  Gly Gly Pro Lys Glu  Val Gly Arg Ile Arg  Ile Ser Gly 
    1355                 1360                 1365             


Asn Met  Pro Lys Ala Arg Glu  Leu Lys Leu Ile His  Tyr Ser Ile 
    1370                 1375                 1380             


Thr Gly  Met Tyr Lys Lys Glu  Ile Asn Leu Leu Asp  
    1385                 1390                 1395 


<210>  1153
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1153

Met Glu Thr Gln Ala Ser Asn Gln Leu Ile Ser Ser His Leu Lys Gly 
1               5                   10                  15      


Tyr Pro Ile Lys Asp Tyr Phe Val Gly Leu Asp Ile Gly Thr Ser Ser 
            20                  25                  30          


Val Gly Trp Ala Val Thr Asn Lys Ala Tyr Glu Leu Leu Lys Phe Arg 
        35                  40                  45              


Ser His Lys Met Trp Gly Ser Arg Leu Phe Asp Glu Gly Glu Ser Ala 
    50                  55                  60                  


Val Ala Arg Arg Gly Phe Arg Ser Met Arg Arg Arg Leu Glu Arg Arg 
65                  70                  75                  80  


Lys Leu Arg Leu Lys Leu Leu Glu Glu Leu Phe Ala Asp Ala Met Ala 
                85                  90                  95      


Gln Val Asp Pro Thr Phe Phe Met Arg Leu Arg Glu Ser Lys Tyr His 
            100                 105                 110         


Tyr Glu Asp Lys Thr Thr Gly His Ser Ser Lys His Ile Leu Phe Ile 
        115                 120                 125             


Asp Lys Asn Tyr Thr Asp Gln Asp Tyr Tyr Lys Glu Tyr Pro Thr Ile 
    130                 135                 140                 


Tyr His Leu Arg Val Ala Leu Met Lys Ser Gly Thr Asp Asp Ile Arg 
145                 150                 155                 160 


Lys Leu Phe Leu Ala Val His His Ile Leu Lys Tyr Arg Gly Asn Phe 
                165                 170                 175     


Leu Tyr Glu Gly Ala Thr Phe Asp Ser Asn Ala Ser Thr Leu Asp Asp 
            180                 185                 190         


Val Leu Lys Gln Ala Leu Glu Asn Ile Thr Phe Asn Cys Phe Asp Ser 
        195                 200                 205             


Ser Ala Ala Ile Gly Ser Ile Gly Gln Ile Leu Met Glu Leu Gly Lys 
    210                 215                 220                 


Thr Lys Ser Asp Lys Ala Lys Ala Ile Glu Arg Leu Val Asp Thr Tyr 
225                 230                 235                 240 


Ile Ile Thr Asp Glu Val Asp Thr Ser Gly Lys Thr Gln Lys Glu Gln 
                245                 250                 255     


Val Lys Glu Asp Lys Lys Arg Leu Lys Ser Phe Ala Asn Leu Val Leu 
            260                 265                 270         


Gly Leu Ser Ala Ser Leu Thr Asp Leu Phe Gly Ser Val Glu Asp Leu 
        275                 280                 285             


Asp Glu Asp Leu Lys Lys Leu Gln Ile Ile Gly Asp Thr Tyr Asp Glu 
    290                 295                 300                 


Arg Arg Asp Asp Leu Ala Lys Val Trp Gly Asp Glu Ile His Ile Ile 
305                 310                 315                 320 


Asp Asp Cys Lys Ser Val Tyr Asp Ala Ile Ile Leu Met Ser Ile Lys 
                325                 330                 335     


Glu Pro Gly Leu Thr Ile Ser Glu Ser Lys Val Lys Ala Phe Lys Lys 
            340                 345                 350         


His Lys Asp Asp Leu Ala Ile Leu Lys Ser Leu Leu Lys Ser Asp Arg 
        355                 360                 365             


Ser Ile Tyr Asn Thr Met Phe Lys Val Asp Glu Lys Gly Leu His Asn 
    370                 375                 380                 


Tyr Val His Tyr Ile Lys Gln Gly Arg Thr Glu Glu Thr Ser Cys Asn 
385                 390                 395                 400 


Arg Glu Asp Phe Tyr Lys Tyr Thr Lys Lys Val Val Glu Gly Leu Pro 
                405                 410                 415     


Asp Ser Lys Asp Lys Glu Tyr Ile Leu Ser Glu Ile Glu Leu Gln Thr 
            420                 425                 430         


Leu Leu Pro Leu Gln Arg Ile Lys Asp Asn Gly Val Ile Pro Tyr Gln 
        435                 440                 445             


Leu His Leu Glu Glu Leu Lys Val Ile Leu Ala Lys Cys Gly Pro Lys 
    450                 455                 460                 


Phe Pro Phe Leu Asn Glu Val Ala Asp Gly Phe Ser Val Ala Glu Lys 
465                 470                 475                 480 


Leu Ile Lys Met Leu Glu Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu 
                485                 490                 495     


Asn Thr His His Asn Val Asp Asn Gly Gly Phe Ala Trp Ala Val Arg 
            500                 505                 510         


Lys Ala Ser Gly Arg Val Thr Pro Trp Asn Phe Asp Asp Lys Ile Asp 
        515                 520                 525             


Arg Glu Lys Ser Ala Ala Ala Phe Ile Lys Asn Leu Thr Asn Lys Cys 
    530                 535                 540                 


Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Lys Ser Ser Leu Leu Tyr 
545                 550                 555                 560 


Ser Glu Phe Met Leu Leu Asn Glu Leu Asn Asn Val Arg Ile Asp Gly 
                565                 570                 575     


Lys Pro Leu Glu Lys Ala Val Lys Glu His Leu Ile Glu Ala Val Phe 
            580                 585                 590         


Lys Gln Asp His Lys Lys Met Thr Lys Asn Arg Ile Glu Gln Phe Leu 
        595                 600                 605             


Lys Asp Asn Asn Tyr Ile Pro Glu Lys His Lys Pro Glu Ile Thr Gly 
    610                 615                 620                 


Leu Asp Gly Glu Ile Lys Asn Asp Leu Ala Ser Tyr Arg Asp Met Val 
625                 630                 635                 640 


Arg Ile Leu Gly Asn Asn Phe Asp Leu Ser Met Ala Glu Glu Ile Ile 
                645                 650                 655     


Thr Asp Ile Thr Ile Phe Gly Glu Ser Lys Lys Met Leu Arg Glu Thr 
            660                 665                 670         


Leu Arg Asn Lys Phe Ser Ser Arg Leu Asp Asp Glu Ala Ile Lys Lys 
        675                 680                 685             


Leu Ser Lys Leu Arg Tyr Arg Asp Trp Gly Arg Leu Ser Arg Lys Leu 
    690                 695                 700                 


Leu Asn Val Ile Glu Gly Cys Asp Lys Ala Gly Asp Gly Thr Pro Glu 
705                 710                 715                 720 


Thr Ile Ile Ile Leu Met Arg Asn Phe Ser Tyr Asn Leu Met Glu Leu 
                725                 730                 735     


Leu Gly Asp Lys Phe Ser Phe Met Glu Arg Ile Gln Glu Ile Asn Ala 
            740                 745                 750         


Lys Leu Ile Glu Gly Gln Ile Val Asn Pro His Asp Ile Ile Asp Asp 
        755                 760                 765             


Leu Ala Leu Ser Pro Ala Val Lys Arg Ala Val Trp Gln Ala Leu Arg 
    770                 775                 780                 


Ile Val Asp Glu Ile Val His Ile Lys Lys Thr Leu Pro Ala Arg Ile 
785                 790                 795                 800 


Phe Val Glu Val Thr Arg Ser Asn Lys Ala Glu Lys Lys Lys Lys Asp 
                805                 810                 815     


Ser Arg Gln Lys Arg Leu Ser Asp Leu Tyr Ala Ala Ile Lys Lys Asp 
            820                 825                 830         


Asp Ala Leu Leu Ser Gly Leu Thr Asn Glu Glu Phe Gly Gly Leu Lys 
        835                 840                 845             


Ser Gly Leu Ala Lys Tyr Asp Asp Ala Ala Leu Arg Ser Lys Lys Leu 
    850                 855                 860                 


Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Cys Ala Tyr Thr Gly Glu Val 
865                 870                 875                 880 


Ile Asp Leu Ser Gln Leu Asn Thr Asp Asn Tyr Asp Ile Asp His Ile 
                885                 890                 895     


Tyr Pro Arg Ser Leu Thr Lys Asp Asp Ser Phe Asp Asn Leu Val Leu 
            900                 905                 910         


Cys Lys Arg Thr Ala Asn Ala Gln Lys Ser Asp Thr Tyr Pro Ile Ser 
        915                 920                 925             


Lys Glu Ile Gln Lys Ala Gln Lys Ala Phe Trp Ile Phe Leu Lys Gln 
    930                 935                 940                 


Gln Gly Leu Ile Ser Glu Arg Lys Tyr Glu Arg Leu Thr Arg Ile Thr 
945                 950                 955                 960 


Pro Leu Thr Ala Asp Asp Leu Ser Gly Phe Ile Ala Arg Gln Leu Val 
                965                 970                 975     


Glu Thr Asn Gln Ser Val Lys Ala Ala Thr Thr Leu Leu Arg Arg Leu 
            980                 985                 990         


Tyr Pro Gly Val Asp Val Val Phe  Val Lys Ala Glu Asn  Val Thr Asp 
        995                 1000                 1005             


Phe Arg  His Asp Asn Asp Phe  Ile Lys Val Arg Ser  Leu Asn His 
    1010                 1015                 1020             


His His  His Ala Lys Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn 
    1025                 1030                 1035             


Val Tyr  His Glu Arg Phe Thr  Arg Asn Phe Arg Ala  Phe Phe Lys 
    1040                 1045                 1050             


Lys Asn  Gly Ala Asn Arg Thr  Tyr Asn Leu Ala Lys  Met Phe Asn 
    1055                 1060                 1065             


Tyr Asp  Val Lys Cys Thr Asn  Ala Lys Asp Gly Lys  Ala Trp Ala 
    1070                 1075                 1080             


Val Lys  Thr Ser Met Asp Thr  Val Lys Lys Met Met  Asp Ser Asn 
    1085                 1090                 1095             


Asp Val  Arg Val Thr Lys Arg  Val Tyr Glu Gln Lys  Gly Gln Leu 
    1100                 1105                 1110             


Ser Asp  Ile Asn Leu Thr Lys  Ala Ser Lys Val Lys  Ala Asp Ile 
    1115                 1120                 1125             


Tyr Leu  Pro Ile Lys Ala Asn  Asp Pro Ile Met Ser  Gln Asn Met 
    1130                 1135                 1140             


Lys Tyr  Gly Gly Tyr Ser Asp  Ile Lys Asn Ala Tyr  Tyr Thr Ile 
    1145                 1150                 1155             


Ile Glu  Tyr Ser Asp Lys Lys  Asn Asn Ser Ile Lys  Ala Phe Ile 
    1160                 1165                 1170             


Pro Ile  Pro Ile Phe Ile Ala  Thr Lys Thr Leu Thr  Lys Gln Ala 
    1175                 1180                 1185             


Leu Leu  Glu Tyr Ile Lys Thr  Val Leu Pro Lys Ala  Asn Asn Leu 
    1190                 1195                 1200             


Arg Ile  Ile Tyr Lys Lys Val  Tyr Asn Asp Gln Leu  Leu Lys Ile 
    1205                 1210                 1215             


Asp Gly  Phe Asn Tyr Tyr Leu  Gly Gly Arg Ser Asn  Asp Arg Phe 
    1220                 1225                 1230             


Cys Ile  Asp Asn Ala Ile Gln  Leu Ile Val Pro Leu  Glu Ile Gln 
    1235                 1240                 1245             


Phe Tyr  Ile Lys Leu Val Glu  Lys Phe Thr Ala Ile  Ile Ser Glu 
    1250                 1255                 1260             


Asn Lys  Asn Ile Ala Leu Lys  Ala Ser Ser Val Lys  Ser Arg Asn 
    1265                 1270                 1275             


Pro Ile  Asn Asn Gln Val Glu  Asn Ile Thr Leu Glu  Tyr Thr Gln 
    1280                 1285                 1290             


Lys Leu  Phe Asp Tyr Leu Val  Ser Lys Phe Asn Glu  Leu Ile Tyr 
    1295                 1300                 1305             


Leu Lys  Met Arg Gly Asn Lys  Leu Thr Asp Val Asn  Asn Ile Gly 
    1310                 1315                 1320             


Arg Lys  Gln Phe Ile Lys Leu  Ser Leu Glu Glu Arg  Cys Lys Ala 
    1325                 1330                 1335             


Leu Ala  Glu Leu Leu Asn Ile  Phe Thr Asn Met Asn  Lys Lys Leu 
    1340                 1345                 1350             


Ser Leu  Ser Thr Ile Asn Val  Ser Ala Ser Arg Lys  Val Val Asn 
    1355                 1360                 1365             


Phe Lys  Ile Ser Ser Leu Asp  Glu Phe Lys Ile Val  Asn Glu Ser 
    1370                 1375                 1380             


Ile Thr  Gly Leu Tyr Ser His  Glu Ile Thr Ile Val  
    1385                 1390                 1395 


<210>  1154
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1154

Met Asn Leu Arg Asn Thr Thr Asp Glu Tyr Asn Ile Gly Leu Asp Met 
1               5                   10                  15      


Gly Thr Asn Ser Val Gly Trp Ala Val Thr Asp Lys Val Gly Ala Ile 
            20                  25                  30          


Ala His Phe Lys Gly Met Pro Thr Leu Gly Ser Arg Leu Phe Asp Ala 
        35                  40                  45              


Ala Gln Thr Ala Ala Glu Ala Arg Ala His Arg Gly Gln Arg Arg Arg 
    50                  55                  60                  


Tyr Val Arg Arg Arg Trp Arg Leu Asp Leu Leu Gln Ser Leu Phe Gln 
65                  70                  75                  80  


Asp Glu Ile Asp Lys Val Asp Ser Glu Phe Phe Ile Arg Leu Arg Gln 
                85                  90                  95      


Ser Arg Leu His Val Glu Asp Arg Glu Asp Gly His Ala Asp Tyr Lys 
            100                 105                 110         


Trp Pro Ile Phe Asn Asp Ser Asp Phe Thr Glu Val Asp Tyr Tyr Lys 
        115                 120                 125             


Arg Phe Pro Thr Ile Tyr His Leu Arg Lys Trp Leu Ile Glu Thr Asp 
    130                 135                 140                 


Gln Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Val His Asn Ile Val 
145                 150                 155                 160 


Lys His Arg Gly Asn Phe Leu Arg Glu Asn Asn Lys Asn Leu Ser Ser 
                165                 170                 175     


Arg Asn Ala Asp Pro Ala Glu Ala Val Gly His Leu Arg Leu Ala Leu 
            180                 185                 190         


Asn Asn Trp Cys Leu Glu Arg Gly Tyr Glu Cys Ala Trp Lys Ser Gly 
        195                 200                 205             


Gly Asn Asp Asn Ala Ile Leu Asn Val Leu Ile Asp Gly Glu Lys Arg 
    210                 215                 220                 


Arg Ser Asp Gln Val Arg Glu Ile Ala Lys Leu Thr Gly Ile Asp Cys 
225                 230                 235                 240 


Gly Asp Ala Lys Gln Ser Arg Gln Cys Asn Glu Ala Ile Ala Lys Ala 
                245                 250                 255     


Ile Val Gly Leu Lys Ala Glu Met Lys Asn Ile Phe Gly Glu Phe Pro 
            260                 265                 270         


Ala Glu Lys Thr Leu Ile Tyr Leu Ser Ser Asp Asp Asp Val Glu Val 
        275                 280                 285             


Val Arg Asp Ala Cys Pro Asp Asp Ser Val Glu Leu Phe Gln Ala Leu 
    290                 295                 300                 


Cys Glu Leu Tyr Ala Ala Tyr Ile Leu Gln Gly Leu Leu Ser Tyr Ala 
305                 310                 315                 320 


Pro Gly Gln Thr Ile Ser Val Asn Met Val Lys Lys Tyr Asp Arg Tyr 
                325                 330                 335     


Gly Glu Asp Leu His Leu Leu Lys Glu Leu Thr Arg Thr Tyr Ala Ser 
            340                 345                 350         


Lys Arg Tyr Asp Ala Phe Phe Arg Gly Glu Thr Tyr Glu Gly Ser Ser 
        355                 360                 365             


Asp Tyr Val Val Asp Lys Ala Gln Gly Tyr Thr Arg Tyr Asn Leu Gly 
    370                 375                 380                 


Thr Thr Gln Leu Ser Tyr Asp Asp Phe Gln Lys Ala Val Lys Glu Leu 
385                 390                 395                 400 


Phe Lys Gly Thr Asp Ala Leu Gln Asp Glu Arg Tyr Gln Gly Met Met 
                405                 410                 415     


Val Ala Phe Gly Glu Gln Arg Phe Leu Arg Arg Leu Lys Thr Ser Asp 
            420                 425                 430         


Asn Gly Ser Ile Tyr Tyr Gln Leu His Leu Glu Glu Leu His Ala Ile 
        435                 440                 445             


Leu Glu Asn Gln Gly Lys His Tyr Pro Phe Leu Leu Ala Glu Glu Asp 
    450                 455                 460                 


Lys Ile Glu Ser Leu Val Ser Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
465                 470                 475                 480 


Leu Thr Arg Glu Asn Ala Ala Val Gly His Asp Gly Lys Glu Arg Phe 
                485                 490                 495     


Ala Trp Ser Ser Arg Arg Asp Gly Met Glu His Ala Val Ile Thr Pro 
            500                 505                 510         


Trp Asn Trp Glu Glu Ile Ile Asp Arg Asp Lys Ser Ala Glu Asp Phe 
        515                 520                 525             


Ile Ile Arg Met Thr Gly Asp Cys Thr Tyr Leu Gln Gly Glu Pro Val 
    530                 535                 540                 


Leu Pro Lys Cys Ser Leu Leu Tyr Glu Glu Phe Cys Ala Leu Asn Glu 
545                 550                 555                 560 


Leu Asn Gly Ala His Trp Thr Ile Asp Glu Asp Asp Glu Arg Arg Phe 
                565                 570                 575     


Asp Ala Ala Gln Arg Glu Arg Ile Met Cys Asp Leu Phe Arg Ser Thr 
            580                 585                 590         


Arg Lys Val Thr Tyr Lys Lys Val Ser Asp Trp Leu Glu Arg Glu Asp 
        595                 600                 605             


Ser Phe Val Gly Ala Arg Val His Gly Gly Gln Gly Glu His Gly Phe 
    610                 615                 620                 


Glu Ser Gln Leu Gly Ser Tyr Ile Phe Phe Cys Lys Asp Val Phe Lys 
625                 630                 635                 640 


Thr Asp Ser Leu Asp Glu Ala Asp Tyr Pro Met Ile Glu Gln Ile Ile 
                645                 650                 655     


Leu Trp Ser Thr Leu Phe Glu Asp Arg Asp Ile Leu Arg Arg Lys Ile 
            660                 665                 670         


Glu Glu Ala Tyr Gly Asp Arg Leu Thr Thr Glu Gln Ile Lys Val Ile 
        675                 680                 685             


Cys Lys Lys Arg Phe Ala Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu 
    690                 695                 700                 


Thr Gly Ile Lys Val Asp Thr Asp Thr Gly Arg Lys Ser Ile Ile Asp 
705                 710                 715                 720 


Val Leu Arg Glu Gly Asn Pro Asn Asp Gly Gln Arg Ser Arg Thr Met 
                725                 730                 735     


Val Phe Met Glu Val Leu Arg Asp Glu Gly Leu Gly Phe Gln Lys Ala 
            740                 745                 750         


Val Asp Asp Phe Asn Arg Asp Tyr Phe Ser Glu His Asn Asn Gly Leu 
        755                 760                 765             


Met Asp Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Arg Arg Ser Ile 
    770                 775                 780                 


Asn Gln Ala Val Arg Ile Val Asp Glu Ile Val Ser Ile Ala Lys Lys 
785                 790                 795                 800 


Glu Pro Ala Asn Ile Phe Ile Glu Val Thr Arg Glu Glu Asp Asp Pro 
                805                 810                 815     


Arg Lys Lys Gly Lys Arg Thr Lys Arg Arg Tyr Asp Ala Ile Lys Ser 
            820                 825                 830         


Ala Leu Glu Val Phe Lys Lys Asp Asp Pro Gln Leu Trp Lys Glu Leu 
        835                 840                 845             


Thr Lys Lys Asp Ser Asn Asn Met Asp Glu Arg Leu Ser Leu Tyr Phe 
    850                 855                 860                 


Met Gln Arg Gly Lys Cys Met Tyr Ser Gly Arg Pro Ile Lys Ile Glu 
865                 870                 875                 880 


Glu Leu His Thr Gly Lys Tyr Glu Val Asp His Ile Ile Pro Arg Ser 
                885                 890                 895     


Tyr Ile Lys Asp Asp Ser Leu Glu Asn Lys Val Leu Val Tyr Arg Glu 
            900                 905                 910         


Glu Asn Gln His Lys Thr Asp Thr Leu Leu Ile Asp Ser Ser Ile Arg 
        915                 920                 925             


Arg Lys Met Gly Lys Tyr Trp Arg Met Leu His Asn Ala Lys Leu Ile 
    930                 935                 940                 


Gly Asp Lys Lys Phe Arg Asn Leu Phe Arg Asp His Ile Asp Asp Lys 
945                 950                 955                 960 


Thr Met Lys Gly Phe Val Ala Arg Gln Ile Val Glu Thr Ser Gln Met 
                965                 970                 975     


Val Lys Leu Val Gln Thr Leu Leu Glu Ala Arg Tyr Pro Gly Ala Arg 
            980                 985                 990         


Ile Val Pro Val Lys Ala Asn Ile  Ser His Asp Leu Arg  Asp Ala Ala 
        995                 1000                 1005             


Gly Leu  Val Lys Cys Arg Glu  Ala Asn Asp Phe His  His Ala His 
    1010                 1015                 1020             


Asp Ala  Phe Leu Ala Cys Arg  Val Gly Leu Phe Ile  Gln Lys Arg 
    1025                 1030                 1035             


His Pro  Ser Val Tyr Asp Asn  Pro Ile Gly Leu Ala  His Ile Met 
    1040                 1045                 1050             


Arg Asp  Tyr Ala Arg Ala Gln  Ala Arg Glu Tyr Lys  Lys Ser His 
    1055                 1060                 1065             


Tyr Leu  Ala His Thr Lys Gly  Phe Ile Val Asn Ser  Phe Leu Ser 
    1070                 1075                 1080             


Ser Gly  Phe Asp Lys Glu Thr  Gly Glu Val Phe Lys  Asp Asp Trp 
    1085                 1090                 1095             


Asp Ala  Glu Ala Glu Val Glu  Gly Ile Arg Arg Val  Leu Asn Tyr 
    1100                 1105                 1110             


Arg Gln  Cys Phe Ile Ser Arg  Met Pro Val Glu Ala  Thr Gly Thr 
    1115                 1120                 1125             


Phe Trp  Asp Gln Thr Ile Tyr  Ser Pro Lys Cys Gly  Lys Lys Leu 
    1130                 1135                 1140             


Thr Leu  Pro Leu Lys Arg Gly  Leu Asp Pro Asp Arg  Tyr Gly Ser 
    1145                 1150                 1155             


Tyr Ser  Arg Glu Gln Phe Ala  Tyr Phe Phe Ile Tyr  Glu Ala Arg 
    1160                 1165                 1170             


Asp Leu  Lys His Glu Arg His  Ile Leu Gln Phe Ala  Gln Val Pro 
    1175                 1180                 1185             


Val Arg  Val Ala Ala His Ile  Ala Lys Asp Pro Ser  Gln Leu Glu 
    1190                 1195                 1200             


Ser Tyr  Ala Val Lys Leu Ala  Gly Gln Ala Asn Leu  Ala Phe Val 
    1205                 1210                 1215             


Arg Ile  Val Arg Arg Arg Ile  Leu Lys Lys Gln Leu  Leu Glu Leu 
    1220                 1225                 1230             


Asn Gly  Asp Arg Leu Ile Ile  Thr Gly Val Arg Glu  Val Arg Asn 
    1235                 1240                 1245             


Ala Thr  Glu Phe Ala Phe Ser  Leu Asp Glu Glu Thr  Leu Ile Glu 
    1250                 1255                 1260             


Arg Cys  Val Gln Ile Ala Lys  Val Gly Ser Leu Ile  Gly Val Val 
    1265                 1270                 1275             


Asn Asp  Glu Asp Glu Glu Gln  Leu Ser Ala Asp Leu  Asn Glu Leu 
    1280                 1285                 1290             


Phe Ser  Ser Leu Val Lys Arg  Gly Pro Lys Leu Cys  Asn Arg Leu 
    1295                 1300                 1305             


Tyr Ala  Gln Leu Lys Leu Asp  Lys Tyr Leu Ser Lys  Phe Asp Glu 
    1310                 1315                 1320             


Leu Thr  Leu Thr Asp Lys Ala  Lys Val Val Leu Gln  Leu Ile Ala 
    1325                 1330                 1335             


Ile Thr  Asn Gly Ser Ile Asn  Met Ala Asp Leu Ser  Ala Leu Gly 
    1340                 1345                 1350             


Ser Ser  Lys Tyr Ala Gly Cys  Met Gln Val Ser Tyr  Phe Lys Gln 
    1355                 1360                 1365             


Leu Asn  Ala Pro Ile Gly Glu  Val Asn Ile Ile Asp  Gln Ser Val 
    1370                 1375                 1380             


Thr Gly  Met Phe Glu Arg Arg  Thr His Ile Gly Leu  
    1385                 1390                 1395 


<210>  1155
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1155

Met Lys Glu Ile Lys Asp Tyr Ile Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Cys Gly Tyr Val Val Thr Asp Lys Gln Asn Asn Ile Leu Lys Leu 
            20                  25                  30          


Lys Gly Lys Thr Ala Ile Gly Ala Arg Leu Phe Lys Glu Gly Gln Ala 
        35                  40                  45              


Ala Ala Asp Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ala Arg 
    50                  55                  60                  


Arg His Trp Arg Leu Gly Leu Leu Glu Glu Ile Phe Asp Pro Glu Met 
65                  70                  75                  80  


Glu Lys Ile Asp Pro Asn Phe Phe Arg Arg Leu Lys Glu Ser Asp Tyr 
                85                  90                  95      


Ser Pro Lys Asp Ser Arg Lys Gln Phe Asn Ala Ile Val Phe Lys Ser 
            100                 105                 110         


Ile Lys Ala Asp Lys Arg Phe Tyr Lys Lys Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Asn Ala Leu Met His Asp Asn Gln Lys His Asp Leu Arg Glu 
    130                 135                 140                 


Ile Phe Met Ala Val His His Ile Val Lys Tyr Arg Gly Asn Phe Leu 
145                 150                 155                 160 


Arg Glu Asp Ser Val Asn Ala Phe Lys Ala Ala Lys Phe Ser Leu Arg 
                165                 170                 175     


Gly Glu Asp Gly Ile Gly Pro Val Asp Lys Leu Asn Asp Leu Leu Lys 
            180                 185                 190         


Glu Ile Tyr Ser Glu Tyr Ser Pro Glu Leu Ala Thr Asn Asn Leu Ser 
        195                 200                 205             


Lys Ile Glu Asp Ile Ile Lys Asp Lys Asn Leu Tyr Lys Gln Asp Lys 
    210                 215                 220                 


Leu Lys Gln Val Ala Thr Leu Leu Ile Lys Glu Ala Asp Ser Lys Asp 
225                 230                 235                 240 


Lys Ala Lys Leu Asn Lys Asp Ile Ala Lys Gln Val Ala Asn Ser Phe 
                245                 250                 255     


Met Gly Tyr Met Phe Arg Leu Asp Thr Leu Phe Ser Leu Thr Asp Val 
            260                 265                 270         


Asp Val Lys Asp Tyr Lys Leu Lys Phe Ser Asp Ala Asn Ile Asp Glu 
        275                 280                 285             


Ser Leu Asp Asp Leu Thr Ser Leu Leu Thr Asp Ala Gln Ile Glu Phe 
    290                 295                 300                 


Val Leu Glu Leu Gln Ser Ile Tyr Asn Thr Ile Val Leu Asn Glu Ile 
305                 310                 315                 320 


Val Pro Asp Gly Met Ser Leu Ser Glu Ser Met Ile Lys Lys Tyr Asp 
                325                 330                 335     


Asp His Lys Glu Asp Leu Lys Ile Phe Lys Glu Tyr Ile Asp Ser Leu 
            340                 345                 350         


Ser Asp Gln Lys Lys Ala Lys Lys Leu Leu Ser Ala Tyr Asn Leu Tyr 
        355                 360                 365             


Val Asn Tyr Arg Lys Ser Asp Leu Val Glu Ala Lys Asn Leu Phe Lys 
    370                 375                 380                 


Asn Lys Lys Val Gly Asp Lys Asn Phe Ala Asp Val Ile Ser Asn Phe 
385                 390                 395                 400 


Glu Val Phe Gly Lys Phe Val Ser Asp Asn Leu Asp Asp Ser Glu Leu 
                405                 410                 415     


Ala Asn Lys Ile Lys Ala Arg Leu Asp Leu Gly Glu Phe Leu Pro Lys 
            420                 425                 430         


Gln Arg Thr Asn Gln Asn Gly Val Ile Pro Tyr Gln Leu His Gln Val 
        435                 440                 445             


Glu Leu Thr Gln Ile Leu Glu Lys Gln Gly Lys Tyr Tyr Pro Phe Leu 
    450                 455                 460                 


Ile Thr Pro Asn Pro Val Glu Ser His Arg Asn Asn Ala Pro Tyr Glu 
465                 470                 475                 480 


Ile Ser Glu Leu Val Ser Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu 
                485                 490                 495     


Ile Asp Asn Gln Ser Ile Lys Asp Lys Gln Asn Lys Asn Lys Phe Ala 
            500                 505                 510         


Trp Met Val Arg Gln Lys Gln Gly Gln Ile Thr Pro Trp Asn Leu Glu 
        515                 520                 525             


Glu Met Val Asp Thr Thr Glu Ser Ala Asn Gln Phe Ile Lys Arg Met 
    530                 535                 540                 


Thr Arg Lys Asp Thr Tyr Leu Leu Ala Glu Asp Val Leu Pro Lys Ser 
545                 550                 555                 560 


Ser Leu Val Tyr Gln Lys Phe Met Ile Leu Asp Glu Leu Asn Arg Ile 
                565                 570                 575     


Lys Ile Asp Gly Lys Lys Leu Thr Ser Glu Leu Lys His Asp Ile Phe 
            580                 585                 590         


Glu Lys Leu Phe Lys Lys Gln Lys Ser Ile Asn Leu Asp Asn Leu Lys 
        595                 600                 605             


Asn Tyr Leu Leu Val Glu Gly Asn Ile Pro Gly Leu Ile Glu Gly Leu 
    610                 615                 620                 


Ser Asp Gly Ile Asn Phe Asn Asn Ser Phe Ser Thr Tyr Ile Asp Tyr 
625                 630                 635                 640 


Arg Asn Ile Phe Gly Asp Glu Ile Asp Asn Pro Asn Lys Gln Ala Asp 
                645                 650                 655     


Phe Glu Lys Met Ile Glu Trp Ser Thr Val Phe Glu Asp Arg Lys Ile 
            660                 665                 670         


Phe Lys Arg Lys Leu Lys Glu Ile Thr Trp Leu Thr Ser Glu Gln Ile 
        675                 680                 685             


Lys Gln Val Ser Ser Lys Arg Tyr Ser Gly Trp Gly Arg Leu Ser Lys 
    690                 695                 700                 


Lys Leu Leu Thr Gln Ile Thr Asp Glu Asn Gly Val Asn Ile Leu Gln 
705                 710                 715                 720 


Arg Leu Trp Asn Glu Pro Glu Thr Leu Thr Glu Val Leu Ala Asn Pro 
                725                 730                 735     


Val Ile Lys Arg Lys Ile Val Glu Ala Asn Ser Ser Phe Thr Lys Ala 
            740                 745                 750         


Lys Asp Met Lys Asp Ile Leu Val Glu Ala Tyr Thr Ser Pro Gln Asn 
        755                 760                 765             


Lys Lys Ala Ile Arg Gln Val Met Arg Val Val Asp Asp Ile Ile Ala 
    770                 775                 780                 


Ala Ala His Gly Lys Lys Pro Ser Gln Ile Ala Ile Glu Phe Ala Arg 
785                 790                 795                 800 


Glu Asp Gln Asp Asn Pro Gln Met Thr Lys Ser Arg Lys Ala Gln Leu 
                805                 810                 815     


Asp Gln Ile Tyr Ala Lys Ile Ser Asp Glu Phe Leu Asp Asp Ala Val 
            820                 825                 830         


Lys Asn Glu Leu Lys Asn Met Lys Asp Asn Lys Pro Leu Ala Lys Asp 
        835                 840                 845             


Lys Val Phe Leu Tyr Phe Lys Gln Met Gly Arg Asp Ala Tyr Ser Gly 
    850                 855                 860                 


Glu Lys Leu Ser Leu Asp Asn Leu Gln Asp Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Tyr Pro Gln Ser Phe Ile Lys Asp Asp Ser Phe Asp Asn Arg Val Leu 
                885                 890                 895     


Val Gln Arg Ala Tyr Asn Asn Gly Lys Ser Asp Asn Val Pro Val Leu 
            900                 905                 910         


Leu Phe Gly Asn Lys Ile Ala Ala Gly Leu Gly Ile Thr Ile Arg Gln 
        915                 920                 925             


Met Trp Lys Lys Trp Leu Glu Leu Gly Leu Ile Ser Lys Arg Lys Phe 
    930                 935                 940                 


Asn Asn Leu Ile Thr Asp Pro Glu Lys Ile Ser Arg Asn Met Ala Ser 
945                 950                 955                 960 


Gly Phe Val Asn Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu 
                965                 970                 975     


Val Ala Asn Ile Leu Gln Ala Lys Leu Pro Asp Thr Glu Ile Ile Glu 
            980                 985                 990         


Val Lys Ala Ser Tyr Asn Ser Ile  Leu Arg Lys Glu Phe  Asn Leu Tyr 
        995                 1000                 1005             


Lys Ser  Arg Glu Val Asn Asp  Tyr His His Ala Ile  Asp Ala Tyr 
    1010                 1015                 1020             


Leu Thr  Thr Ile Val Gly Asn  Tyr Leu Tyr Gln Val  Tyr Pro Lys 
    1025                 1030                 1035             


Leu Arg  Pro Tyr Phe Val Tyr  Gly Gln Phe Lys Lys  Phe Gly Lys 
    1040                 1045                 1050             


Ala Glu  Asn Gln Glu Asp Asp  Pro Val Lys Lys Ile  Lys Lys Phe 
    1055                 1060                 1065             


Asn Phe  Ile Tyr Gln Leu Ile  Trp Gly Lys Asp Asp  Ala Ile Cys 
    1070                 1075                 1080             


Ile Ser  His Thr Asn Gln Gln  Val Phe Ser Lys Lys  Asp Ile Ile 
    1085                 1090                 1095             


Asp Lys  Leu Thr Thr Ala Tyr  Asn Phe Lys Tyr Met  Asn Ile Ser 
    1100                 1105                 1110             


His Ala  Val Tyr Thr Arg Asn  Asn Asn Met Phe Lys  Gln Thr Leu 
    1115                 1120                 1125             


Phe Pro  Ile Ile Glu Arg Thr  Thr Ala Lys Thr Lys  Ser Leu Ile 
    1130                 1135                 1140             


Pro Lys  Lys Leu Asp Arg Pro  Thr Asp Ile Tyr Gly  Gly Tyr Ser 
    1145                 1150                 1155             


Asn Asn  Asn Asp Ala Tyr Leu  Ala Leu Val Lys Val  Asp Lys Lys 
    1160                 1165                 1170             


Arg Gly  Ser Glu Tyr Arg Val  Val Gly Ile Pro Met  Arg Ala Ala 
    1175                 1180                 1185             


Ala Met  Leu Lys Ala Ser Lys  Asn Tyr Asp Glu Asp  Leu Arg Lys 
    1190                 1195                 1200             


Val Ile  Glu Pro Met Val Met  Phe Asp Lys Asn Gly  Lys Ala Lys 
    1205                 1210                 1215             


Arg Gly  Ile Glu Gly Phe Arg  Val Leu Val Gly Lys  Met Pro Phe 
    1220                 1225                 1230             


Lys Gln  Ala Val Leu Asp Gly  Asn Lys Lys Phe Met  Leu Thr Gly 
    1235                 1240                 1245             


Thr Val  Asp Thr Pro Asn Leu  Lys Gln Leu Val Leu  Ser Gln Gln 
    1250                 1255                 1260             


Asn Met  Lys Tyr Ile Leu Asp  Tyr Ile Glu Asp Val  Asp Cys Arg 
    1265                 1270                 1275             


Lys His  Lys Phe Gly Asp Lys  Val Gln Asn Pro Asp  Lys Cys Leu 
    1280                 1285                 1290             


Leu Glu  Val Phe Asp Glu Ile  Ile Glu Ile Val Asp  Lys Tyr Phe 
    1295                 1300                 1305             


Glu Leu  Phe Asp Ser Arg Asp  Phe Arg Glu Lys Leu  Arg Asn Ser 
    1310                 1315                 1320             


Arg Asn  Lys Phe Ile Lys Leu  Glu Ala Ser Lys Lys  Ala Asp Thr 
    1325                 1330                 1335             


Ile Leu  Ala Ile Leu Lys Gly  Leu Gln Ala Lys Ser  Thr Arg Ala 
    1340                 1345                 1350             


Thr Ile  Lys Glu Leu Asn Ile  Ser Asp Phe Gly Arg  Ile Ile Thr 
    1355                 1360                 1365             


Ser Ser  Ile Ser Pro Glu Ala  Gln Leu Ile Tyr Gln  Ser Pro Thr 
    1370                 1375                 1380             


Gly Leu  Phe Glu Arg Arg Val  Lys Val Lys Asp Leu  
    1385                 1390                 1395 


<210>  1156
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1156

Met Ser Lys Lys Tyr Gln Asn Thr Lys Tyr Tyr Val Gly Leu Asp Ile 
1               5                   10                  15      


Gly Thr Ser Ser Val Gly Trp Ala Val Thr Asp Ala Asn Tyr Glu Leu 
            20                  25                  30          


Leu Lys Val Asn Gly His Lys Thr Trp Gly Ser Arg Leu Phe Thr Glu 
        35                  40                  45              


Ala Glu Thr Ala Leu Glu Arg Arg Thr His Arg Ala Thr Arg Arg Arg 
    50                  55                  60                  


Leu Gln Arg Arg Lys Gln Arg Leu Asp Leu Leu Asp Glu Leu Phe Ala 
65                  70                  75                  80  


Glu Glu Leu Ala Lys Val Asp Pro Gln Phe Leu Leu Arg Leu Lys Glu 
                85                  90                  95      


Ser Gln Tyr His Met Glu Asp Lys Ser Thr Asp Asp Lys Tyr Val Leu 
            100                 105                 110         


Phe Asn Asp Gln Asn Arg Thr Asp Gly Glu Phe Phe Thr Glu Tyr Pro 
        115                 120                 125             


Thr Ile Tyr His Leu Arg Ser Glu Leu Met Gln Gly Lys Lys Glu Asp 
    130                 135                 140                 


Ile Arg Ala Val Tyr Leu Ala Leu His His Ile Val Lys Tyr Arg Gly 
145                 150                 155                 160 


His Phe Leu Leu Pro Gly Asp Asn Tyr Asp Met Gln Gly Asn Leu Ser 
                165                 170                 175     


Glu Leu Ile Lys Arg Leu Phe Lys Phe Ile Glu Glu Thr Leu Phe Ile 
            180                 185                 190         


Glu Asp Leu Gly Leu Thr Asp Glu Ile Gln Ser Lys Ile Glu Glu Ile 
        195                 200                 205             


Leu Leu Thr Lys Thr Lys Thr Arg Leu Asp Arg Gln Lys Glu Ile Val 
    210                 215                 220                 


Lys Leu Phe Asn Thr Lys His Gln Lys Leu Asp Asp Lys Gln Lys Lys 
225                 230                 235                 240 


Leu Leu Glu Ala Ile Phe Lys Leu Cys Leu Gly Leu Lys Gly Asn Met 
                245                 250                 255     


Val Asn Ile Phe Gly Asp Lys Gly Glu Tyr Gly Ile Asp Phe Thr Asp 
            260                 265                 270         


Val Glu Asp Lys Lys Lys Lys Val Glu Phe Ser Ala Asp Tyr Asp Asn 
        275                 280                 285             


Asn Ile Asn Asp Tyr Val Asp Phe Phe Gly Glu Tyr Phe Glu Leu Leu 
    290                 295                 300                 


Glu Ile Thr Lys Lys Ile Asn Asp Thr Val Ile Phe Ser Glu Ile Leu 
305                 310                 315                 320 


Val Asp Gly Lys Gly Phe Ser Glu Ser Lys Val Ile Ser Tyr Glu Lys 
                325                 330                 335     


His Gln Gln Asp Leu Arg Lys Leu Lys Thr Leu Leu Lys Ser Leu Asp 
            340                 345                 350         


Lys Thr Leu Gly Tyr Lys Tyr Gly Glu Gly Leu Tyr Gln Lys Ile Leu 
        355                 360                 365             


Leu Asp Asp Val Lys Ser Asn Tyr Val Asn Tyr Ser Gly Asn Ser Gln 
    370                 375                 380                 


Lys Leu Lys Lys Cys Thr Lys Asp Glu Phe Tyr Gly Phe Ile Lys Ser 
385                 390                 395                 400 


Lys Leu Asn Ser Val Thr Lys Lys Glu Lys Val Ser Glu Ala Gln Gln 
                405                 410                 415     


Lys Glu Ile Ala Gln Ile Leu Ala Asp Met Glu Phe Gly Asp Tyr Leu 
            420                 425                 430         


Pro Leu Gln Arg Thr Thr Asp Asn Gly Val Ile Pro Asn Gln Leu His 
        435                 440                 445             


Lys Lys Glu Leu Ile Glu Ile Ile Glu Gln Gln Thr Lys Tyr His Asp 
    450                 455                 460                 


Phe Phe Asn Asp Val Asp Glu Asn Gly Leu Thr Ile Lys Asp Lys Ile 
465                 470                 475                 480 


Ile Ser Leu Met Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn 
                485                 490                 495     


Asp Tyr His Lys Val Ala Asp Gly Thr Gly His Ala Trp Ile Glu Lys 
            500                 505                 510         


Lys Lys Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Glu Met Val Asp 
        515                 520                 525             


Thr Glu Ala Thr Ala Glu Lys Phe Ile Lys Asn Leu Thr Asn Lys Cys 
    530                 535                 540                 


Thr Tyr Leu Lys Gly Glu Asp Val Leu Pro Lys Glu Ser Lys Leu Tyr 
545                 550                 555                 560 


Ser Glu Phe Met Leu Leu Asn Glu Leu Asn Asn Ile Lys Ile Asp Asn 
                565                 570                 575     


Gln Lys Leu Asp Pro Lys Leu Lys Met Lys Met Val Ser Glu Leu Phe 
            580                 585                 590         


Met Lys Lys Asn Glu Lys Ile Thr Lys Lys Lys Ile Val Asp Tyr Leu 
        595                 600                 605             


Arg Ser Ile Gly Gln Ala Asn Gly Asn Glu Met Val Ser Gly Ile Asp 
    610                 615                 620                 


Arg Glu Ile Lys Ser Asp Leu Lys Thr Ile His Lys Ile Gln His Tyr 
625                 630                 635                 640 


Tyr Gln Asp Ala Ser Leu Glu Met Ala Glu Asn Ile Ile Arg Trp Asn 
                645                 650                 655     


Thr Ile Phe Asn Asp Ser Thr Ser Asp Ile Leu Lys Asp Lys Ile Thr 
            660                 665                 670         


Lys Ala Tyr Gly Asp Val Leu Thr Pro Gly Gln Ile Lys Gly Leu Ser 
        675                 680                 685             


Asn Leu Ala Cys Lys Asp Trp Gly Lys Leu Ser Glu Lys Phe Leu Thr 
    690                 695                 700                 


Gly Ile Arg Cys Asp Val Gly Glu Phe Lys Asn Ile Ser Ile Ile Gln 
705                 710                 715                 720 


Ala Met Arg Asp Thr Asn Asn Asn Leu Met Glu Leu Leu Ser Asn Lys 
                725                 730                 735     


Tyr Glu Phe Met Asp Lys Ile Gln Glu Tyr Asn Lys Glu Tyr Tyr Gly 
            740                 745                 750         


Asp Phe Thr Phe Lys Ala Glu Pro Ser Ile Met Asp Asp Leu Tyr Val 
        755                 760                 765             


Gly Pro Gly Pro Lys Arg Ala Ile Trp Gln Ala Val Arg Ile Val Glu 
    770                 775                 780                 


Glu Ile Lys Arg Ile Met Gly His Asp Pro Glu Lys Ile Phe Leu Glu 
785                 790                 795                 800 


Thr Thr Arg Thr Asn Arg Ala Pro Lys Val Lys Asn Thr Lys Arg Tyr 
                805                 810                 815     


Asp Ala Leu Glu Lys Lys Tyr Glu Ser Val Lys Glu His Glu Tyr Ala 
            820                 825                 830         


Glu Val Lys Lys Glu Phe Glu Ile Glu Lys Asn Asp Ser Leu Asn Ile 
        835                 840                 845             


Lys Lys Leu Phe Leu Tyr Phe Thr Gln Leu Gly His Cys Met Tyr Ser 
    850                 855                 860                 


Asp Glu Lys Ile Pro Tyr Asn Glu Leu Phe Thr Asn Arg Tyr Asp Ile 
865                 870                 875                 880 


Asp His Ile Tyr Pro Arg Ser Lys Thr Arg Asp Asn Ser Met Asp Asn 
                885                 890                 895     


Leu Val Leu Val Lys Ala Glu Cys Asn Arg Asp Lys Lys Asp Asn Tyr 
            900                 905                 910         


Pro Ile Lys Leu Lys Thr Asn Ile Gln Gln Lys Cys Gln Val Arg Trp 
        915                 920                 925             


Ala Tyr Leu Arg Asp Lys Lys Leu Ile Thr Glu Glu Lys Tyr Asn Arg 
    930                 935                 940                 


Leu Val Arg Lys Thr Pro Leu Thr Glu Asp Glu Leu Ser Gln Phe Ile 
945                 950                 955                 960 


Ala Arg Gln Leu Val Phe Thr Ser Gln Ser Val Lys Ala Thr Ala Glu 
                965                 970                 975     


Val Phe Lys Arg Leu Tyr Glu Asp Ser Lys Ile Cys Tyr Val Lys Ala 
            980                 985                 990         


Glu Asn Val Ser Asp Phe Arg Tyr  Gly Ser Thr Arg Lys  Met Ser Lys 
        995                 1000                 1005             


Asp Asp  Gln Pro Gln Pro Phe  Ile Lys Asn Arg Asp  Val Asn Asp 
    1010                 1015                 1020             


Phe His  His Ala Lys Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn 
    1025                 1030                 1035             


Val Tyr  Asp Val Lys Phe Thr  Gln Asn Pro Thr Arg  Phe Ile Lys 
    1040                 1045                 1050             


Ala Asn  Lys Asp Tyr Ser Leu  Asn Tyr Ile Phe Arg  Tyr Pro Val 
    1055                 1060                 1065             


Asn Lys  Lys Gly Glu Asn Val  Trp Asp Pro Glu Gln  Ser Met Lys 
    1070                 1075                 1080             


Ile Val  Glu Lys Thr Leu Lys  Ser Asn Asp Val Arg  Val Thr Lys 
    1085                 1090                 1095             


Leu Val  Lys Pro Ile Thr Ser  Pro Ile Ser Asn Glu  Thr Leu His 
    1100                 1105                 1110             


Lys Ala  Leu Lys Cys Lys Pro  Gly Ser Tyr Leu Pro  Arg Lys Ser 
    1115                 1120                 1125             


Ser Lys  Gly Thr Pro Leu Ala  Asp Val Thr Lys Tyr  Gly Gly Tyr 
    1130                 1135                 1140             


Glu Lys  Ile Lys Asn Ala Tyr  Tyr Ser Ile Tyr Lys  Tyr Thr Tyr 
    1145                 1150                 1155             


Met Lys  Lys Lys Glu Cys Met  Glu Glu Phe Leu Leu  Val Pro Ile 
    1160                 1165                 1170             


Pro Ile  His Leu Val Asn Asn  Asp Glu Glu Leu Pro  Lys Tyr Ile 
    1175                 1180                 1185             


Asn Asp  Lys Ile Ala Glu Arg  Ala Lys Lys Lys Lys  Leu Glu Thr 
    1190                 1195                 1200             


Val Glu  Cys Ile Val Lys Glu  Leu Tyr Gln Gly Thr  Leu Val Arg 
    1205                 1210                 1215             


Ile Asn  Gly Tyr Asp Tyr Arg  Ile Ala Gly Ser Ser  Gly Lys Gln 
    1220                 1225                 1230             


Phe Glu  Leu His Asn Gly Ile  Asn Leu Ile Val Glu  Pro Trp Val 
    1235                 1240                 1245             


Glu Lys  Val Leu Lys Glu Thr  Asp Lys Phe Val Arg  Lys Ser Asn 
    1250                 1255                 1260             


Ser Lys  Leu Asp Lys Lys Gln  Gln Lys Glu Asn Thr  Thr Glu Thr 
    1265                 1270                 1275             


Leu Ile  Glu Lys Gln Leu Asn  Asp Val Phe Glu Tyr  Leu Cys Arg 
    1280                 1285                 1290             


Lys Leu  Asn Gln Gly Ile Phe  Lys Asn Asn Lys His  Thr Leu Pro 
    1295                 1300                 1305             


Ala Lys  Leu Leu Gly Asp Ser  Ile Gln Lys Arg Phe  Glu Asp Ala 
    1310                 1315                 1320             


Ser Ile  Glu Asp Glu Phe Glu  Val Ile Lys Glu Leu  Ile Ser Ala 
    1325                 1330                 1335             


Ile Ala  Lys Asn Pro Gln Asn  Phe Lys Thr Ile Gln  Lys Val Leu 
    1340                 1345                 1350             


Asp Ile  Ser Gly Ser Ser Ile  Pro Arg Phe Asn Leu  Lys Leu Thr 
    1355                 1360                 1365             


Lys Asn  Asn Val Asp Glu Phe  Ile Ile Lys Asn Glu  Ser Val Thr 
    1370                 1375                 1380             


Gly Leu  Phe Thr His Glu Lys  Val Ile Phe Arg Lys  
    1385                 1390                 1395 


<210>  1157
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1157

Met Lys Lys Glu Ile Lys Asp Tyr Phe Leu Gly Leu Asp Val Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asp Thr Asp Tyr Lys Leu Leu Lys 
            20                  25                  30          


Ala Asn Arg Lys Asp Leu Trp Gly Met Arg Cys Phe Glu Thr Ala Glu 
        35                  40                  45              


Thr Ala Glu Val Arg Arg Leu His Arg Gly Ala Arg Arg Arg Ile Glu 
    50                  55                  60                  


Arg Arg Lys Lys Arg Ile Lys Leu Leu Gln Glu Leu Phe Ser Gln Glu 
65                  70                  75                  80  


Ile Ala Lys Ile Asp Glu Gly Phe Phe Gln Arg Met Lys Glu Ser Pro 
                85                  90                  95      


Leu Tyr Ala Glu Asp Lys Thr Ile Leu Gln Glu Asn Thr Leu Phe Asn 
            100                 105                 110         


Asp Lys Asp Phe Thr Asp Lys Thr Tyr His Lys Ala Tyr Pro Thr Ile 
        115                 120                 125             


Asn His Leu Ile Lys Ala Trp Ile Glu Asn Lys Val Lys Pro Asp Pro 
    130                 135                 140                 


Arg Leu Leu Tyr Leu Ala Cys His Asn Ile Ile Lys Lys Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Glu Gly Asp Phe Asp Ser Glu Asn Gln Phe Asp Thr Ser 
                165                 170                 175     


Ile Gln Ala Leu Phe Glu Tyr Leu Arg Glu Asp Met Glu Leu Asp Ile 
            180                 185                 190         


Asp Ala Asp Ser Gln Lys Val Lys Glu Ile Leu Lys Asp Ser Ser Leu 
        195                 200                 205             


Lys Asn Ser Glu Lys Gln Ser Arg Leu Asn Lys Met Leu Gly Leu Lys 
    210                 215                 220                 


Pro Ser Asp Lys Gln Lys Lys Ala Ile Thr Asn Leu Ile Ser Gly Asn 
225                 230                 235                 240 


Lys Ile Asn Phe Ala Asp Leu Tyr Asp Asn Pro Asp Leu Lys Asp Ala 
                245                 250                 255     


Glu Lys Asn Ser Ile Ser Phe Ser Lys Asp Asp Phe Asp Ala Leu Ser 
            260                 265                 270         


Asp Asp Leu Ala Ser Ile Leu Gly Asp Ser Phe Glu Leu Leu Leu Lys 
        275                 280                 285             


Ala Lys Ala Val Tyr Asn Cys Ser Val Leu Ser Lys Val Ile Gly Asp 
    290                 295                 300                 


Glu Gln Tyr Leu Ser Phe Ala Lys Val Lys Ile Tyr Glu Lys His Lys 
305                 310                 315                 320 


Thr Asp Leu Thr Lys Leu Lys Asn Val Ile Lys Lys His Phe Pro Lys 
                325                 330                 335     


Asp Tyr Lys Lys Val Phe Gly Tyr Asn Lys Asn Glu Lys Asn Asn Asn 
            340                 345                 350         


Asn Tyr Ser Ala Tyr Val Gly Val Cys Lys Ile Thr His Lys Lys Leu 
        355                 360                 365             


Val Ile Lys Asn Ser Ala Ser Gln Glu Asp Phe Tyr Lys Phe Leu Lys 
    370                 375                 380                 


Thr Ile Leu Ser Ala Lys Ser Glu Ile Lys Glu Val Asn Asp Ile Leu 
385                 390                 395                 400 


Thr Glu Ile Glu Thr Gly Thr Phe Leu Pro Lys Gln Ile Ser Lys Ser 
                405                 410                 415     


Asn Ala Glu Ile Pro Tyr Gln Leu Arg Lys Met Glu Leu Glu Lys Ile 
            420                 425                 430         


Leu Ser Asn Ala Glu Lys His Phe Ser Phe Leu Lys Gln Lys Asp Glu 
        435                 440                 445             


Lys Gly Leu Ser His Ser Glu Lys Ile Ile Met Leu Leu Thr Phe Lys 
    450                 455                 460                 


Ile Pro Tyr Tyr Ile Gly Pro Ile Asn Asp Asn His Lys Lys Ser Phe 
465                 470                 475                 480 


Pro Asp Arg Cys Trp Val Val Lys Lys Glu Lys Ser Pro Ser Gly Lys 
                485                 490                 495     


Thr Thr Pro Trp Asn Phe Phe Asp His Ile Asp Lys Glu Lys Thr Ala 
            500                 505                 510         


Glu Glu Phe Ile Thr Ser Arg Thr Asn Phe Cys Thr Tyr Leu Ile Gly 
        515                 520                 525             


Glu Ser Val Leu Pro Lys Ser Ser Leu Leu Tyr Ser Glu Tyr Thr Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Leu Gln Ile Ile Ile Asp Gly Lys Asn Ile 
545                 550                 555                 560 


Cys Asp Ile Lys Leu Lys Gln Lys Ile Tyr Glu Glu Leu Phe Lys Lys 
                565                 570                 575     


Tyr Lys Lys Ile Thr Gln Lys Gln Ile Ser Thr Phe Ile Lys His Glu 
            580                 585                 590         


Gly Ile Cys Asn Lys Thr Asp Glu Val Ile Ile Leu Gly Ile Asp Lys 
        595                 600                 605             


Glu Cys Thr Ser Ser Leu Lys Ser Tyr Ile Glu Leu Lys Asn Ile Phe 
    610                 615                 620                 


Gly Lys Gln Val Asp Glu Ile Ser Thr Lys Asn Met Leu Glu Glu Ile 
625                 630                 635                 640 


Ile Arg Trp Ala Thr Ile Tyr Asp Glu Gly Glu Gly Lys Thr Ile Leu 
                645                 650                 655     


Lys Thr Lys Ile Lys Ala Glu Tyr Gly Lys Tyr Cys Ser Asp Glu Gln 
            660                 665                 670         


Ile Lys Lys Ile Leu Asn Leu Lys Phe Ser Gly Trp Gly Arg Leu Ser 
        675                 680                 685             


Arg Lys Phe Leu Glu Thr Val Thr Ser Glu Met Pro Gly Phe Ser Glu 
    690                 695                 700                 


Pro Val Asn Ile Ile Thr Ala Met Arg Glu Thr Gln Asn Asn Leu Met 
705                 710                 715                 720 


Glu Leu Leu Ser Ser Glu Phe Lys Phe Thr Glu Asn Ile Lys Lys Ile 
                725                 730                 735     


Asn Ser Gly Phe Glu Asp Ala Glu Lys Gln Phe Ser Tyr Asn Gly Leu 
            740                 745                 750         


Val Lys Pro Leu Phe Leu Ser Pro Ser Val Lys Lys Met Leu Trp Gln 
        755                 760                 765             


Thr Leu Lys Leu Val Lys Glu Ile Ser His Ile Thr Gln Ala Pro Pro 
    770                 775                 780                 


Lys Lys Ile Phe Ile Glu Met Ala Lys Gly Ala Glu Leu Glu Pro Ala 
785                 790                 795                 800 


Arg Thr Lys Thr Arg Leu Lys Ile Leu Gln Asp Leu Tyr Ser Ser Cys 
                805                 810                 815     


Lys Asn Asp Ala Asp Ala Phe Ser Ser Glu Ile Gln Tyr Leu Ser Gly 
            820                 825                 830         


Lys Ile Glu Asn Glu Asp Asn Leu Arg Leu Arg Ser Asp Lys Leu Tyr 
        835                 840                 845             


Leu Tyr Tyr Thr Gln Leu Gly Lys Cys Met Tyr Cys Gly Lys Pro Ile 
    850                 855                 860                 


Glu Ile Gly His Val Phe Asp Thr Ser Asn Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile Ser Asn Arg Val Leu 
                885                 890                 895     


Val Cys Ser Ser Cys Asn Lys Asn Lys Glu Asp Lys Tyr Pro Leu Lys 
            900                 905                 910         


Ala Glu Ile Gln Ser Lys Gln Arg Gly Phe Trp Asn Phe Leu Gln Arg 
        915                 920                 925             


Asn Asn Phe Ile Ser Leu Glu Lys Leu Asn Arg Leu Thr Arg Thr Ser 
    930                 935                 940                 


Pro Ile Ser Asp Asp Glu Thr Ala Lys Phe Ile Ala Arg Gln Leu Val 
945                 950                 955                 960 


Glu Thr Arg Gln Ala Thr Lys Val Ala Ala Lys Val Leu Glu Lys Met 
                965                 970                 975     


Phe Pro Glu Thr Lys Ile Val Tyr Ser Lys Ala Glu Thr Val Ser Met 
            980                 985                 990         


Phe Arg Asn Lys Phe Asp Ile Ile  Lys Cys Arg Glu Ile  Asn Asp Phe 
        995                 1000                 1005             


His His  Ala His Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val 
    1010                 1015                 1020             


Tyr Asn  Thr Lys Phe Thr Asn  Asn Pro Trp Asn Phe  Ile Lys Glu 
    1025                 1030                 1035             


Lys Arg  Asp Asn Pro Lys Ile  Ala Asp Thr Tyr Asn  Tyr Tyr Lys 
    1040                 1045                 1050             


Val Phe  Asp Tyr Asp Val Lys  Arg Asn Asn Ile Thr  Ala Trp Glu 
    1055                 1060                 1065             


Lys Gly  Lys Thr Ile Ile Thr  Val Lys Asp Met Leu  Lys Arg Asn 
    1070                 1075                 1080             


Thr Pro  Ile Tyr Thr Arg Gln  Ala Ala Cys Lys Lys  Gly Glu Leu 
    1085                 1090                 1095             


Phe Asn  Gln Thr Ile Met Lys  Lys Gly Leu Gly Gln  His Pro Leu 
    1100                 1105                 1110             


Lys Lys  Glu Gly Pro Leu Ser  Asn Ile Ser Lys Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Asn Lys  Val Ser Ala Ala Tyr  Tyr Thr Leu Ile Glu  Tyr Glu Glu 
    1130                 1135                 1140             


Lys Gly  Ser Lys Ile Arg Ser  Leu Glu Thr Ile Pro  Leu Tyr Leu 
    1145                 1150                 1155             


Val Lys  Asp Ile Gln Lys Asn  Lys Asp Gly Leu Lys  Ser Tyr Leu 
    1160                 1165                 1170             


Thr Asp  Gln Ile Gly Lys Lys  Glu Ile Lys Ile Leu  Ile Pro Lys 
    1175                 1180                 1185             


Ile Lys  Ile Asn Ser Leu Leu  Lys Ile Asn Gly Phe  Pro Cys His 
    1190                 1195                 1200             


Ile Thr  Gly Lys Met Gly Glu  Ala Phe Val Leu Arg  Pro Ala Val 
    1205                 1210                 1215             


Gln Phe  Cys Cys Ser Asn Asp  Glu Ala Leu Tyr Phe  Lys Lys Ile 
    1220                 1225                 1230             


Ile Arg  Phe Asn Glu Ile Arg  Lys Gln Arg Glu Lys  Met Gly Lys 
    1235                 1240                 1245             


Thr Ile  Ser Pro Tyr Glu Asp  Leu Ser Phe Arg Ser  Tyr Ile Lys 
    1250                 1255                 1260             


Asp Asn  Leu Cys Lys Lys Thr  Lys Ala Asp Glu Ile  Glu Glu Lys 
    1265                 1270                 1275             


Glu Phe  Tyr Asp Leu Leu Gln  Lys Lys Asn Leu Glu  Ile Tyr Asp 
    1280                 1285                 1290             


Met Leu  Leu Thr Lys His Lys  Asp Thr Ile Tyr Lys  Lys Arg Pro 
    1295                 1300                 1305             


Asn Ser  Ala Thr Ile Asp Ile  Leu Val Lys Gly Lys  Glu Lys Phe 
    1310                 1315                 1320             


Lys Ser  Leu Ile Ile Glu Asn  Gln Phe Glu Val Ile  Leu Glu Ile 
    1325                 1330                 1335             


Leu Lys  Leu Phe Ser Ala Thr  Arg Asn Val Ser Asp  Leu Gln His 
    1340                 1345                 1350             


Ile Gly  Gly Ser Lys Asn Ala  Gly Asn Ala Arg Val  Gly Asn Lys 
    1355                 1360                 1365             


Ile Ser  Ser Leu Asp Asn Cys  Ile Leu Ile Tyr Gln  Ser Ile Thr 
    1370                 1375                 1380             


Gly Ile  Phe Glu Lys Arg Ile  Asp Leu Leu Lys Ile  
    1385                 1390                 1395 


<210>  1158
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1158

Met Ser Phe Lys Lys Ile Leu Gly Leu Asp Val Gly Val Ala Ser Ile 
1               5                   10                  15      


Gly Trp Ala Val Val Arg Glu Asn Glu Glu Asn Pro Asn Phe Asn Thr 
            20                  25                  30          


Ile Thr Lys Leu Gly Val Arg Val Val Pro Ile Thr Ser Asp Glu Lys 
        35                  40                  45              


Leu Asn Phe Glu Lys Gly Lys Ser Ile Thr Ser Asn Ala Glu Arg Thr 
    50                  55                  60                  


Leu Ala Arg Ser Ser Arg Arg Asn Leu Gln Arg Phe Arg Leu Arg Arg 
65                  70                  75                  80  


Lys Asn Leu Ile Ala Ile Leu Leu Gln Asn Gln Ile Ile Thr Pro Thr 
                85                  90                  95      


Thr Pro Leu Thr Glu Lys Gly Lys Phe Thr Thr Phe Glu Thr Leu Lys 
            100                 105                 110         


Ile Arg Ala Lys Ala Thr Lys Glu Lys Ile Glu Leu Glu Glu Leu Ala 
        115                 120                 125             


Arg Val Leu Leu His Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg 
    130                 135                 140                 


Lys Val Lys Asn Ser Glu Asp Gly Lys Ala Ile Asp Gly Met Glu Ala 
145                 150                 155                 160 


Ala Lys Ile Leu Tyr Glu Asn Lys Leu Thr Pro Gly Gln Tyr Thr Tyr 
                165                 170                 175     


Gln Leu Leu Glu Lys Gly Ser Asn Lys Ile Pro Asp Phe Tyr Cys Ser 
            180                 185                 190         


Asp Leu Gln Ile Glu Trp Glu Val Ile Trp Asn Phe Gln Lys Gln Phe 
        195                 200                 205             


Tyr Pro Asn Leu Leu Thr Glu Asp Phe Lys Lys Glu Leu Lys Gly Ile 
    210                 215                 220                 


Gly Gln Arg Ala Thr Ala Ala Leu Phe Leu Lys Lys Tyr Gly Ile Tyr 
225                 230                 235                 240 


Thr Ala Leu Asn Lys Gly Lys Arg Glu Glu Lys Lys Leu Gln Ala Tyr 
                245                 250                 255     


Lys Trp Arg Ser Lys Ser Val Ser Glu Lys Ile Pro Val Glu Glu Leu 
            260                 265                 270         


Ala Tyr Val Ile Ala Glu Ile Asn Ser His Ile Thr Asn Ser Ser Gly 
        275                 280                 285             


Tyr Leu Gly Thr Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Gln 
    290                 295                 300                 


Gln Thr Ile Gly Glu Asn Leu Tyr Ala Gln Val Ala Lys Asn Thr His 
305                 310                 315                 320 


Ala Lys Leu Lys Asn Gln Val Phe Leu Arg Gln Asp Tyr Ser Ala Glu 
                325                 330                 335     


Phe Glu Gln Ile Trp Glu Thr Gln Ala Lys Phe His Pro Gln Leu Thr 
            340                 345                 350         


Pro Glu Leu Lys Lys Lys Ile Arg Asp Ser Ile Ile Phe Tyr Gln Arg 
        355                 360                 365             


Lys Leu Arg Ser Gln Lys Arg Leu Ile Ser Ile Cys Glu Phe Glu Asn 
    370                 375                 380                 


Arg Glu Ile Glu Ile Ile Lys Asn Gly Lys Lys Lys Lys Lys Lys Ile 
385                 390                 395                 400 


Gly Leu Lys Ala Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys 
                405                 410                 415     


Ile Trp Gln Val Leu Asn Asn Ile Val Ile Lys Gln Glu Gly Lys Asp 
            420                 425                 430         


Arg Leu Leu Thr Trp Glu Glu Lys Glu Leu Leu Phe Thr Glu Leu Asn 
        435                 440                 445             


Ile Lys Gly Asn Ile Ser Gly Gln Lys Ala Leu Ala Leu Leu Lys Leu 
    450                 455                 460                 


Ser Pro Lys Asp Thr Lys Ile Asn Val Thr Ile Leu Glu Gly Asn His 
465                 470                 475                 480 


Thr Gln Glu Gln Leu Tyr Arg Val Tyr Leu Arg Ile Leu Gly Met Glu 
                485                 490                 495     


Gly Tyr Asp Val Phe Asp Leu Leu Lys Ile Pro Glu Asn Gln Asp Glu 
            500                 505                 510         


Ile Lys Pro Ala Asp Ser Lys Ile Ser Ala Ala Glu Ile Lys Glu Thr 
        515                 520                 525             


Val Lys Ser Val Phe Lys Thr Cys Ser Ile Ser Pro Ser Ile Leu Asp 
    530                 535                 540                 


Phe Asp Pro Leu Leu Pro Asn Pro Glu Phe Glu Asn Gln Ser Ser Tyr 
545                 550                 555                 560 


Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Ser Ser Pro Ser 
                565                 570                 575     


Gly Asn Glu Lys Leu Leu Glu Leu Leu Lys Asn Lys Phe Gly Phe Lys 
            580                 585                 590         


Lys Glu His Ala Leu Leu Leu Ala Ser Ile Ser Tyr Thr Tyr Ile Tyr 
        595                 600                 605             


Gly Asn Leu Ser Ser Lys Ala Met Arg Lys Ile Tyr Pro Tyr Ser Lys 
    610                 615                 620                 


Glu Asn Thr Tyr Ser Glu Ala Cys Met Leu Ala Gly Tyr Arg His Ser 
625                 630                 635                 640 


Lys Asn Ala Leu Pro Arg Glu Glu Leu Val Lys Tyr Gln Leu Lys Asp 
                645                 650                 655     


Arg Leu Glu Ile Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
            660                 665                 670         


Lys Val Leu Asn Gln Leu Ile Asn Leu Val Asn Ser Leu Met Asp Glu 
        675                 680                 685             


Tyr Gly Ala Phe Asp Glu Ile Arg Leu Glu Met Ala Arg Glu Leu Lys 
    690                 695                 700                 


Lys Asn Ala Lys Glu Arg Glu Lys Trp Ser Lys Asn Ile Ala Asp Ala 
705                 710                 715                 720 


Thr Ser Arg Asn Gln Lys His Ala Glu Ile Leu Lys Lys Asp Phe Lys 
                725                 730                 735     


Ile Ser Asn Pro Ser Lys Asn Asp Leu Ile Arg Tyr Arg Leu Tyr Leu 
            740                 745                 750         


Glu Leu Lys Ala Asn Gly Tyr Asn Asp Leu Tyr Thr Asn Thr Tyr Ile 
        755                 760                 765             


Asp Ile Asn Tyr Leu Phe Thr His Arg Tyr Asp Ile Asp His Ile Ile 
    770                 775                 780                 


Pro Gln Ser Lys Val Leu Asp Asp Ser Phe Ser Asn Lys Val Leu Val 
785                 790                 795                 800 


Pro Arg Gln Ala Asn Leu Glu Lys Ser Asn Leu Thr Ala Phe Asp Tyr 
                805                 810                 815     


Ile Ser His Gln Gly Lys Glu Lys Glu Glu His Tyr Leu Asn Val Ile 
            820                 825                 830         


Thr Glu Leu Tyr Lys Arg Gly Ser Ile Ser Glu Thr Lys Tyr Lys Asn 
        835                 840                 845             


Leu Gln Lys Lys Gly Asn Glu Ile Gly Glu Gly Phe Ile Asn Arg Asp 
    850                 855                 860                 


Leu Lys Asn Thr Gln Tyr Ile Val Arg Lys Ala Arg Glu Ile Leu Ser 
865                 870                 875                 880 


Leu Ile Thr Pro Asn Val Val Thr Thr Thr Gly Glu Ile Thr Ala Lys 
                885                 890                 895     


Leu Arg Lys Asp Trp Asn Leu Ile Asn Ser Met Lys Glu Leu Asn Met 
            900                 905                 910         


Asp Lys Phe Arg Asn Ala Arg Leu Thr Glu Leu Val Glu Asn Ser Arg 
        915                 920                 925             


Gly Glu Lys Lys Glu Ile Ile Ile Asp Trp Asn Lys Arg Asn Asp Gln 
    930                 935                 940                 


Arg His His Ala Ile Asp Ala Leu Val Val Ala Phe Thr Thr Arg Asn 
945                 950                 955                 960 


His Val Gln Tyr Leu Asn Tyr Met Asn Ala Arg Glu Asp Glu Ser His 
                965                 970                 975     


Lys Gln His Ser His Ile Leu Ser Ile Glu Lys Lys Ile Lys Tyr Asp 
            980                 985                 990         


Lys Phe His Glu Asn Gly Ser Arg  Gln Ser Arg Phe Ile  Tyr Pro Thr 
        995                 1000                 1005             


Ser Asn  Phe Arg Glu Glu Ala  Lys Lys Lys Leu Lys  Glu Ile Leu 
    1010                 1015                 1020             


Val Ser  Tyr Lys Thr Lys Asn  Lys Val Val Thr Val  Asn Ile Asn 
    1025                 1030                 1035             


Lys Pro  Lys Lys Lys Gly Gly  Tyr Ser Pro Lys His  Glu Leu Thr 
    1040                 1045                 1050             


Pro Arg  Gly Pro Leu His Lys  Glu Thr Ile Tyr Gly  Met Ile Arg 
    1055                 1060                 1065             


Ile Leu  His Thr Lys Glu Ile  Lys Val Ser Ser Lys  Leu Asn Phe 
    1070                 1075                 1080             


Glu Thr  Val Val Arg Ile Gln  Asn Pro Arg Tyr Arg  Asn Ala Leu 
    1085                 1090                 1095             


Leu Glu  Arg Leu Lys Asp Asn  Phe Asn Asp Pro Lys  Lys Ala Phe 
    1100                 1105                 1110             


Ser Gly  Thr Asn Ser Leu Thr  Lys Asn Pro Val Phe  Leu Thr Ala 
    1115                 1120                 1125             


Asn Ser  Thr Glu Thr Val Pro  Glu Lys Val Thr Ile  Gln Trp Phe 
    1130                 1135                 1140             


Glu Lys  Val Tyr Thr Ile Arg  Lys Pro Ile Asn Pro  Thr Asn Phe 
    1145                 1150                 1155             


Lys Asn  Glu Lys Asn Ile Glu  Lys Val Leu Asp Pro  Thr Ile Arg 
    1160                 1165                 1170             


Thr Leu  Leu Lys Lys Arg Leu  Gln Glu Tyr Asn Gly  Asn Ala Lys 
    1175                 1180                 1185             


Glu Ala  Phe Ser Asp Leu Glu  Lys Asn Pro Ile Trp  Leu Asn Gln 
    1190                 1195                 1200             


Glu Lys  Gly Ile Cys Ile Lys  Thr Val Thr Ile Thr  Gly Ile Ser 
    1205                 1210                 1215             


Asn Val  Glu Ser Leu His Thr  Lys Lys Asp His Phe  Gly Lys Glu 
    1220                 1225                 1230             


Leu Leu  Asp Ile Asn Gly Asn  Pro Ile Pro Val Asp  Phe Val Ser 
    1235                 1240                 1245             


Thr Gly  Asn Asn His His Val  Ala Ile Tyr Glu Asp  Ser Glu Gly 
    1250                 1255                 1260             


Asn Leu  Gln Glu Lys Val Val  Ser Phe Tyr Glu Ala  Val Glu Arg 
    1265                 1270                 1275             


Val Asn  Gln Gly Leu Pro Val  Ile Asp Thr Leu Tyr  Asn Leu Asp 
    1280                 1285                 1290             


Leu Gly  Trp Lys Phe Leu Phe  Thr Met Lys Gln Asn  Glu Met Phe 
    1295                 1300                 1305             


Leu Phe  Pro Ala Asp Asp Phe  Asp Pro Asn Asp Ile  Asp Ile Tyr 
    1310                 1315                 1320             


Asp Glu  Lys Asn Leu Ile Leu  Ile Ser Lys His Leu  Phe Arg Val 
    1325                 1330                 1335             


Gln Lys  Leu Ser Ser Lys Asp  Tyr Cys Phe Arg His  His Leu Glu 
    1340                 1345                 1350             


Thr Ser  Ile Asn Phe Asn Asp  Pro Ala Leu Lys Gly  Ile Thr Trp 
    1355                 1360                 1365             


Arg Arg  Glu Ser Pro Asn Gly  Leu Arg Asn Leu Val  Lys Val Arg 
    1370                 1375                 1380             


Leu Asn  His Leu Gly Lys Ile  Val His Ile Gly Glu  
    1385                 1390                 1395 


<210>  1159
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1159

Met Lys Lys Glu Ile Lys Asp Tyr Phe Leu Gly Leu Asp Val Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asp Thr Asp Tyr Lys Leu Leu Lys 
            20                  25                  30          


Ala Asn His Lys Asp Leu Trp Gly Met Arg Cys Phe Glu Thr Ala Glu 
        35                  40                  45              


Thr Ala Glu Lys Arg Arg Leu Phe Arg Gly Ala Arg Arg Arg Ile Glu 
    50                  55                  60                  


Arg Arg Arg Lys Arg Ile Lys Leu Leu Gln Glu Leu Phe Ser Gln Glu 
65                  70                  75                  80  


Ile Ala Lys Ile Asp Glu Asp Phe Phe Gln Arg Met Glu Glu Ser Pro 
                85                  90                  95      


Phe Tyr Ala Glu Asp Lys Thr Ile Leu Gln Glu Asn Thr Leu Phe Asn 
            100                 105                 110         


Asp Arg Asp Phe Thr Asp Lys Thr Tyr His Lys Ala Tyr Pro Thr Ile 
        115                 120                 125             


Asn His Leu Ile Lys Ala Trp Ile Glu Asn Lys Val Lys Pro Asp Pro 
    130                 135                 140                 


Arg Leu Leu Tyr Leu Ala Cys His Asn Ile Ile Lys Lys Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Glu Gly Asp Phe Asp Ser Glu Asn Gln Phe Asp Thr Ser 
                165                 170                 175     


Val Gln Ala Leu Phe Glu Tyr Leu Arg Glu Asp Met Glu Leu Asp Ile 
            180                 185                 190         


Asp Ala Asp Ser Lys Lys Ile Lys Glu Ile Leu Lys Asp Ser Ala Leu 
        195                 200                 205             


Lys Asn Ser Asp Lys Gln Asn Arg Leu Asn Lys Leu Leu Gly Leu Lys 
    210                 215                 220                 


Ser Ser Asp Lys Gln Lys Lys Ala Leu Thr Asn Leu Ile Ser Gly Asn 
225                 230                 235                 240 


Lys Ala Asn Phe Ala Asp Leu Tyr Asp Asn Pro Asp Leu Lys Asp Ala 
                245                 250                 255     


Glu Lys Asn Ser Ile Ser Phe Ser Lys Asp Asp Phe Asp Ala Leu Ser 
            260                 265                 270         


Asp Glu Leu Ala Ser Val Leu Gly Asp Ser Phe Glu Leu Leu Leu Arg 
        275                 280                 285             


Ala Lys Ala Val Tyr Asn Cys Ser Val Leu Ser Lys Val Ile Gly Asp 
    290                 295                 300                 


Glu Gln Tyr Leu Ser Phe Ala Lys Val Lys Ile Tyr Glu Lys His Lys 
305                 310                 315                 320 


Asn Asp Leu Thr Lys Leu Lys Glu Val Val Lys Lys Tyr Phe Pro Ile 
                325                 330                 335     


Glu Tyr Arg Asn Ile Phe Gly Tyr Lys Gln Ser Glu Asn Asn Asn Cys 
            340                 345                 350         


Asn Tyr Ser Arg Tyr Val Gly Val Cys Lys Ser Gly Ser Lys Lys Lys 
        355                 360                 365             


Val Ile Asn Lys Thr Ala Ser Gln Glu Asp Phe Tyr Lys Phe Leu Lys 
    370                 375                 380                 


Asp Ile Leu Leu Lys Arg Ser Asp Val Pro Glu Ala Asn Gly Ile Leu 
385                 390                 395                 400 


Thr Glu Ile Glu Thr Gly Thr Phe Leu Pro Lys Gln Ile Ser Lys Asn 
                405                 410                 415     


Asn Ala Glu Ile Pro Tyr Gln Leu Arg Lys Ile Glu Leu Glu Lys Ile 
            420                 425                 430         


Leu Thr Asn Ala Glu Lys Phe Phe Ser Phe Leu Lys Gln Lys Asp Glu 
        435                 440                 445             


Asn Gly Leu Ser His Ser Glu Lys Ile Ile Met Leu Leu Thr Phe Lys 
    450                 455                 460                 


Ile Pro Tyr Tyr Ile Gly Pro Ile Asn Thr Ser His Lys Gln Tyr Phe 
465                 470                 475                 480 


Pro Asp Arg Ser Trp Val Ile Lys Lys Glu Asn Ala Pro Gln Glu Lys 
                485                 490                 495     


Ile Thr Pro Trp Asn Phe Phe Asp His Ile Asp Lys Glu Lys Thr Ala 
            500                 505                 510         


Glu Ala Phe Ile Thr Ser Arg Thr Asn Phe Cys Thr Tyr Leu Ile Gly 
        515                 520                 525             


Glu Ser Val Leu Pro Lys Asn Ser Leu Leu Tyr Ser Glu Tyr Thr Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Leu Gln Ile Ile Ile Asp Gly Lys Asn Ile 
545                 550                 555                 560 


Cys Asp Val Lys Leu Lys Gln Lys Ile Tyr Glu Glu Leu Phe Lys Lys 
                565                 570                 575     


Tyr Lys Lys Val Thr Gln Lys Gln Ile Ser Thr Phe Ile Lys His Glu 
            580                 585                 590         


Gly Ile Cys Asn Lys Thr Asp Glu Val Ile Ile Leu Gly Ile Asp Lys 
        595                 600                 605             


Glu Cys Thr Ser Ser Leu Lys Ser Tyr Ile Glu Leu Lys Asn Ile Phe 
    610                 615                 620                 


Gly Lys Gln Val Asp Glu Ile Ser Thr Lys Asn Met Leu Glu Asp Ile 
625                 630                 635                 640 


Ile Arg Trp Ala Thr Ile Tyr Asp Glu Gly Glu Gly Lys Thr Ile Leu 
                645                 650                 655     


Lys Thr Lys Ile Lys Ala Glu Tyr Glu Lys Tyr Tyr Ser Asp Glu Gln 
            660                 665                 670         


Ile Lys Lys Ile Leu Asn Leu Lys Leu Ser Gly Trp Gly Arg Leu Ser 
        675                 680                 685             


Arg Lys Phe Leu Glu Ser Ile Thr Ser Glu Ile Pro Gly Phe Ile Glu 
    690                 695                 700                 


Pro Val Asn Ile Ile Thr Ala Met Arg Glu Thr Gln Asn Asn Leu Met 
705                 710                 715                 720 


Glu Leu Leu Ser Ser Lys Phe Lys Phe Asn Glu Asn Ile Lys Lys Ile 
                725                 730                 735     


Asn Ser Gly Phe Glu Asp Thr Asp Lys Arg Phe Ser Tyr Asn Ser Leu 
            740                 745                 750         


Val Lys Pro Leu Phe Leu Ser Pro Ser Val Lys Lys Met Leu Trp Gln 
        755                 760                 765             


Thr Leu Lys Leu Val Lys Glu Ile Ser His Ile Thr Gln Ala Pro Pro 
    770                 775                 780                 


Lys Lys Ile Phe Ile Glu Met Ala Lys Gly Ala Glu Leu Glu Pro Ala 
785                 790                 795                 800 


Arg Thr Lys Thr Arg Leu Lys Ile Leu Gln Asp Leu Tyr Asn Ser Cys 
                805                 810                 815     


Lys Asn Asp Ala Asp Ala Phe Ser Ser Glu Ile Gln Tyr Leu Leu Gly 
            820                 825                 830         


Lys Ile Glu Asn Glu Asp Asn Leu Arg Leu Arg Ser Asp Lys Leu Tyr 
        835                 840                 845             


Leu Tyr Tyr Thr Gln Leu Gly Lys Cys Met Tyr Cys Gly Lys Pro Ile 
    850                 855                 860                 


Glu Ile Gly His Val Phe Asp Thr Ser Lys Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile Asn Asn Arg Val Leu 
                885                 890                 895     


Val Cys Ser Ser Cys Asn Lys Asn Lys Glu Asp Lys Tyr Pro Leu Lys 
            900                 905                 910         


Pro Glu Ile Gln Leu Lys Gln Lys Lys Phe Trp Asn Phe Leu Gln Arg 
        915                 920                 925             


Asn Asn Phe Ile Ser Ile Glu Lys Phe Asn Arg Leu Thr Arg Thr Thr 
    930                 935                 940                 


Pro Ile Ser Asp Asp Glu Thr Ala Lys Phe Ile Ala Arg Gln Leu Val 
945                 950                 955                 960 


Glu Thr Arg Gln Ala Thr Lys Val Ala Ala Lys Val Leu Glu Lys Ile 
                965                 970                 975     


Phe Pro Glu Thr Asn Ile Val Tyr Ser Lys Ala Glu Thr Val Ser Met 
            980                 985                 990         


Phe Arg Asn Lys Phe Asp Ile Ile  Lys Cys Arg Glu Ile  Asn Asp Phe 
        995                 1000                 1005             


His His  Ala His Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val 
    1010                 1015                 1020             


Tyr Asn  Thr Lys Phe Thr Asn  Asn Pro Trp Asn Phe  Ile Lys Glu 
    1025                 1030                 1035             


Lys Arg  Asp Asn Pro Lys Ile  Gly Asp Thr Tyr Asn  Tyr Tyr Lys 
    1040                 1045                 1050             


Val Phe  Asp Tyr Asp Val Lys  Arg Asn Asn Ile Thr  Ala Trp Glu 
    1055                 1060                 1065             


Lys Glu  Lys Thr Ile Ile Thr  Val Lys Thr Met Leu  Lys Arg Asn 
    1070                 1075                 1080             


Thr Pro  Ile Tyr Thr Arg Gln  Ala Ala Cys Lys Lys  Gly Glu Leu 
    1085                 1090                 1095             


Phe Asn  Gln Thr Ile Met Lys  Lys Gly Leu Gly Gln  His Pro Leu 
    1100                 1105                 1110             


Lys Lys  Glu Cys Pro Phe Ser  Asn Ile Ser Lys Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Asn Lys  Val Ser Ala Ala Tyr  Tyr Thr Phe Ile Glu  Tyr Glu Glu 
    1130                 1135                 1140             


Lys Gly  Asn Lys Ile Arg Ser  Leu Glu Thr Ile Pro  Leu Tyr Leu 
    1145                 1150                 1155             


Glu Lys  Asp Ile Gln Lys Ser  Gln Asp Gly Leu Lys  Gly Tyr Leu 
    1160                 1165                 1170             


Thr Asp  Gln Leu Gly Lys Lys  Glu Ile Lys Ile Leu  Val Pro Lys 
    1175                 1180                 1185             


Ile Lys  Ile Asn Ser Leu Leu  Lys Ile Asn Gly Phe  Pro Cys His 
    1190                 1195                 1200             


Ile Thr  Gly Lys Thr Gly Ala  Ser Phe Val Leu Arg  Pro Ala Val 
    1205                 1210                 1215             


Gln Phe  Cys Cys Ser Asn Asp  Glu Val Leu Tyr Phe  Lys Lys Ile 
    1220                 1225                 1230             


Ile Arg  Phe Asn Glu Ile Arg  Ser Gln Arg Glu Lys  Met Gly Lys 
    1235                 1240                 1245             


Thr Ile  Ser Pro Tyr Glu Asp  Leu Ser Phe Arg Ser  Tyr Ile Lys 
    1250                 1255                 1260             


Glu Asn  Leu Cys Lys Lys Thr  Lys Asn Asp Glu Ile  Gly Glu Lys 
    1265                 1270                 1275             


Glu Phe  Tyr Gly Phe Leu Gln  Lys Lys Asn Leu Glu  Ile Tyr Asp 
    1280                 1285                 1290             


Met Leu  Leu Ile Lys His Lys  Asp Thr Ile Tyr Ser  Lys Arg Pro 
    1295                 1300                 1305             


Asn Ser  Thr Thr Leu Asp Met  Leu Ile Lys Gly Arg  Tyr Glu Phe 
    1310                 1315                 1320             


Ile Asn  Leu Lys Pro Glu Asp  Gln Ile Arg Val Met  Leu Glu Ile 
    1325                 1330                 1335             


Leu Lys  Leu Phe Cys Thr Thr  Arg Glu Val Ile Asp  Leu Gly Leu 
    1340                 1345                 1350             


Ile Lys  Gly Lys Ser Glu Ala  Gly Val Thr Thr Leu  Gly Lys Lys 
    1355                 1360                 1365             


Ile Ser  Asn Leu Asp Ser Cys  Ile Leu Ile Tyr Gln  Ser Ile Thr 
    1370                 1375                 1380             


Gly Ile  Phe Glu Lys Arg Ile  Asp Leu Leu Lys Ile  
    1385                 1390                 1395 


<210>  1160
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1160

Met Lys Lys Glu Ile Lys Asp Tyr Phe Leu Gly Leu Asp Val Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asp Thr Asp Tyr Lys Leu Leu Lys 
            20                  25                  30          


Ala Asn Arg Lys Asp Leu Trp Gly Met Arg Cys Phe Glu Thr Ala Glu 
        35                  40                  45              


Thr Ala Glu Val Arg Arg Leu His Arg Gly Ala Arg Arg Arg Ile Glu 
    50                  55                  60                  


Arg Arg Lys Lys Arg Ile Lys Leu Leu Gln Glu Leu Phe Ser Gln Glu 
65                  70                  75                  80  


Ile Ala Lys Thr Asp Glu Gly Phe Phe Gln Arg Met Lys Glu Ser Pro 
                85                  90                  95      


Phe Tyr Ala Glu Asp Lys Thr Ile Leu Gln Glu Asn Ala Leu Phe Asn 
            100                 105                 110         


Asp Arg Asp Phe Thr Asp Lys Thr Tyr His Lys Ala Tyr Pro Thr Ile 
        115                 120                 125             


Asn His Leu Ile Lys Ala Trp Ile Glu Asn Lys Val Lys Pro Asp Pro 
    130                 135                 140                 


Arg Leu Leu Tyr Leu Ala Cys His Asn Ile Ile Lys Lys Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Glu Gly Asp Phe Asp Ser Glu Asn Gln Phe Asp Thr Ser 
                165                 170                 175     


Ile Gln Ala Leu Phe Glu Tyr Leu Arg Glu Asp Met Glu Val Asp Ile 
            180                 185                 190         


Asp Ala Asp Ser Gln Lys Val Lys Glu Ile Leu Lys Asp Ser Ser Leu 
        195                 200                 205             


Lys Asn Ser Glu Lys Gln Ser Arg Leu Asn Lys Met Leu Gly Leu Lys 
    210                 215                 220                 


Pro Ser Asp Lys Gln Lys Lys Ala Ile Thr Asn Leu Ile Ser Gly Asn 
225                 230                 235                 240 


Lys Ile Asn Phe Ala Asp Leu Tyr Asp Asn Pro Asp Leu Lys Asp Ala 
                245                 250                 255     


Glu Lys Asn Ser Ile Ser Phe Ser Lys Asp Asp Phe Asp Ala Leu Ser 
            260                 265                 270         


Asp Asp Leu Ala Ser Ile Leu Gly Asp Ser Phe Glu Leu Leu Leu Lys 
        275                 280                 285             


Ala Lys Ala Val Tyr Asn Cys Ser Val Leu Ser Lys Val Ile Gly Asp 
    290                 295                 300                 


Glu Gln Tyr Leu Ser Phe Ala Lys Val Lys Ile Tyr Glu Lys His Lys 
305                 310                 315                 320 


Thr Asp Leu Thr Lys Leu Lys Asn Val Ile Lys Lys His Phe Pro Asn 
                325                 330                 335     


Asp Tyr Lys Lys Val Phe Gly Tyr Asn Lys Asn Glu Lys Asn Asn Asn 
            340                 345                 350         


Asn Tyr Ser Ala Tyr Val Gly Val Cys Lys Thr Lys Ser Lys Lys Leu 
        355                 360                 365             


Val Ile Asn Asn Thr Val Asn Gln Glu Asp Phe Tyr Lys Phe Leu Lys 
    370                 375                 380                 


Pro Ile Leu Leu Ala Lys Ser Glu Ile Lys Glu Val Asn Asp Ile Leu 
385                 390                 395                 400 


Thr Glu Ile Glu Thr Gly Thr Phe Leu Pro Lys Gln Ile Ser Lys Ser 
                405                 410                 415     


Asn Ala Glu Ile Pro Tyr Gln Leu Arg Lys Met Glu Leu Glu Lys Ile 
            420                 425                 430         


Leu Ser Asn Ala Glu Lys His Phe Ser Phe Leu Lys Gln Lys Asp Glu 
        435                 440                 445             


Lys Gly Leu Ser His Ser Glu Lys Ile Ile Met Leu Leu Thr Phe Lys 
    450                 455                 460                 


Ile Pro Tyr Tyr Ile Gly Pro Ile Asn Asp Asn His Lys Lys Ser Phe 
465                 470                 475                 480 


Pro Asp Arg Cys Trp Val Ile Lys Lys Glu Lys Ser Pro Ser Gly Lys 
                485                 490                 495     


Thr Thr Pro Trp Asn Phe Phe Asp His Ile Asp Lys Asp Lys Thr Ala 
            500                 505                 510         


Glu Ala Phe Ile Thr Ser Arg Thr Asn Phe Cys Thr Tyr Leu Val Gly 
        515                 520                 525             


Glu Ser Val Leu Pro Lys Asn Ser Leu Leu Tyr Ser Glu Tyr Thr Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Leu Gln Ile Ile Ile Asp Gly Lys Asn Ile 
545                 550                 555                 560 


Cys Asp Ile Lys Leu Lys Gln Lys Ile Tyr Glu Glu Leu Phe Lys Lys 
                565                 570                 575     


Tyr Lys Lys Ile Thr Gln Lys Gln Ile Ser Thr Phe Ile Lys His Glu 
            580                 585                 590         


Gly Ile Cys Asn Lys Thr Asp Glu Val Ile Ile Leu Gly Ile Asp Lys 
        595                 600                 605             


Glu Cys Thr Ser Ser Leu Lys Ser Tyr Ile Glu Leu Lys Asn Ile Phe 
    610                 615                 620                 


Gly Lys Gln Val Asp Glu Ile Ser Thr Lys Asn Met Leu Glu Glu Ile 
625                 630                 635                 640 


Ile Arg Trp Ala Thr Ile Tyr Asp Glu Gly Glu Gly Lys Thr Ile Leu 
                645                 650                 655     


Lys Thr Lys Ile Lys Ala Glu Tyr Gly Lys Tyr Cys Ser Asp Glu Gln 
            660                 665                 670         


Ile Lys Lys Ile Leu Asn Leu Lys Phe Ser Gly Trp Gly Arg Leu Ser 
        675                 680                 685             


Arg Lys Phe Leu Glu Thr Val Thr Ser Glu Met Pro Gly Phe Ser Glu 
    690                 695                 700                 


Pro Val Asn Ile Ile Thr Ala Met Arg Glu Thr Gln Asn Asn Leu Met 
705                 710                 715                 720 


Glu Leu Leu Ser Ser Glu Phe Lys Phe Thr Glu Asn Ile Lys Lys Ile 
                725                 730                 735     


Asn Ser Gly Phe Glu Asp Val Glu Lys Gln Phe Ser Tyr Asp Ser Leu 
            740                 745                 750         


Val Lys Pro Leu Phe Leu Ser Pro Ser Val Lys Lys Met Leu Trp Gln 
        755                 760                 765             


Thr Leu Lys Leu Val Lys Glu Ile Ser His Ile Thr Gln Ala Pro Pro 
    770                 775                 780                 


Lys Lys Ile Phe Ile Glu Met Ala Lys Gly Ala Glu Leu Asp Pro Val 
785                 790                 795                 800 


Arg Thr Lys Thr Arg Leu Lys Ile Leu Gln Asp Leu Tyr Asn Asn Cys 
                805                 810                 815     


Lys Asn Asp Ala Asp Ala Phe Ser Ser Glu Ile Lys Asp Leu Ser Gly 
            820                 825                 830         


Lys Ile Glu Asn Glu Asp Asn Leu Arg Leu Arg Ser Asp Lys Leu Tyr 
        835                 840                 845             


Leu Tyr Tyr Thr Gln Leu Gly Lys Cys Met Tyr Cys Gly Lys Pro Ile 
    850                 855                 860                 


Glu Ile Gly His Val Phe Asp Thr Ser Asn Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile Ser Asn Arg Val Leu 
                885                 890                 895     


Val Cys Ser Ser Cys Asn Lys Asn Lys Glu Asp Lys Tyr Pro Leu Lys 
            900                 905                 910         


Ala Glu Ile Gln Ser Lys Gln Arg Gly Phe Trp Asn Phe Leu Gln Arg 
        915                 920                 925             


Asn Asn Phe Ile Ser Leu Glu Lys Leu Asn Arg Leu Thr Arg Thr Ser 
    930                 935                 940                 


Pro Ile Ser Asp Asp Glu Thr Ala Lys Phe Ile Ala Arg Gln Leu Val 
945                 950                 955                 960 


Glu Thr Arg Gln Ala Thr Lys Val Ala Ala Lys Val Leu Glu Lys Met 
                965                 970                 975     


Phe Pro Glu Thr Lys Ile Val Tyr Ser Lys Ala Glu Thr Val Ser Met 
            980                 985                 990         


Phe Arg Asn Lys Phe Asp Ile Ile  Lys Cys Arg Glu Ile  Asn Asp Phe 
        995                 1000                 1005             


His His  Ala His Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val 
    1010                 1015                 1020             


Tyr Asn  Thr Lys Phe Thr Asn  Asn Pro Trp Asn Phe  Ile Lys Glu 
    1025                 1030                 1035             


Lys Arg  Asp Asn Pro Lys Ile  Ala Asp Thr Tyr Asn  Tyr Tyr Lys 
    1040                 1045                 1050             


Val Phe  Asp Tyr Asp Val Lys  Arg Asn Asn Ile Thr  Ala Trp Glu 
    1055                 1060                 1065             


Lys Gly  Lys Thr Ile Ile Thr  Val Lys Asp Met Leu  Lys Arg Asn 
    1070                 1075                 1080             


Thr Pro  Ile Tyr Thr Arg Gln  Ala Ala Cys Lys Lys  Gly Glu Leu 
    1085                 1090                 1095             


Phe Asn  Gln Thr Ile Met Lys  Lys Gly Leu Gly Gln  His Pro Leu 
    1100                 1105                 1110             


Lys Lys  Glu Gly Pro Leu Ser  Asn Ile Ser Lys Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Asn Lys  Val Ser Ala Ala Tyr  Tyr Thr Leu Ile Glu  Tyr Glu Glu 
    1130                 1135                 1140             


Lys Gly  Ser Lys Ile Arg Ser  Leu Glu Thr Ile Pro  Leu Tyr Leu 
    1145                 1150                 1155             


Val Lys  Asp Ile Gln Lys Asn  Lys Asp Gly Leu Lys  Ser Tyr Leu 
    1160                 1165                 1170             


Thr Asp  Gln Ile Gly Lys Lys  Glu Ile Lys Ile Leu  Ile Pro Lys 
    1175                 1180                 1185             


Ile Lys  Ile Asn Ser Leu Leu  Lys Ile Asn Gly Phe  Pro Cys His 
    1190                 1195                 1200             


Ile Thr  Gly Lys Met Gly Glu  Ala Phe Val Leu Arg  Pro Ala Val 
    1205                 1210                 1215             


Gln Phe  Cys Cys Ser Asn Asp  Glu Ala Leu Tyr Phe  Lys Lys Ile 
    1220                 1225                 1230             


Ile Arg  Phe Asn Glu Ile Arg  Lys Gln Arg Glu Lys  Met Gly Lys 
    1235                 1240                 1245             


Thr Ile  Ser Pro Tyr Glu Asp  Leu Ser Phe Arg Ser  Tyr Ile Lys 
    1250                 1255                 1260             


Asp Asn  Leu Cys Lys Lys Thr  Lys Ala Asp Glu Ile  Glu Glu Lys 
    1265                 1270                 1275             


Glu Phe  Tyr Asp Leu Leu Gln  Lys Lys Asn Leu Glu  Ile Tyr Asp 
    1280                 1285                 1290             


Met Leu  Leu Thr Lys His Lys  Asp Thr Ile Tyr Lys  Lys Arg Pro 
    1295                 1300                 1305             


Asn Ser  Ala Thr Ile Asp Ile  Leu Val Lys Gly Lys  Glu Lys Phe 
    1310                 1315                 1320             


Lys Ser  Leu Ile Ile Glu Asn  Gln Phe Glu Val Ile  Leu Glu Ile 
    1325                 1330                 1335             


Leu Lys  Leu Phe Ser Ala Thr  Arg Asn Val Ser Asp  Leu Gln His 
    1340                 1345                 1350             


Ile Gly  Gly Ser Lys Asn Ala  Gly Asn Ala Arg Val  Gly Asn Lys 
    1355                 1360                 1365             


Ile Ser  Ser Leu Asp Asn Cys  Ile Leu Ile Tyr Gln  Ser Ile Thr 
    1370                 1375                 1380             


Gly Ile  Phe Glu Lys Arg Ile  Asp Leu Leu Lys Ile  
    1385                 1390                 1395 


<210>  1161
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1161

Met Lys Lys Glu Ile Lys Asp Tyr Phe Leu Gly Leu Asp Val Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asp Thr Asp Tyr Lys Leu Leu Lys 
            20                  25                  30          


Ala Asn Arg Lys Asp Leu Trp Gly Met Arg Cys Phe Glu Thr Ala Glu 
        35                  40                  45              


Thr Ala Glu Val Arg Arg Leu His Arg Gly Ala Arg Arg Arg Ile Glu 
    50                  55                  60                  


Arg Arg Lys Lys Arg Ile Lys Leu Leu Gln Glu Leu Phe Ser Gln Glu 
65                  70                  75                  80  


Ile Ala Lys Ile Asp Glu Gly Phe Phe Gln Arg Met Lys Glu Ser Pro 
                85                  90                  95      


Phe Tyr Ala Glu Asp Lys Thr Ile Leu Gln Glu Asn Ala Leu Phe Asn 
            100                 105                 110         


Asp Lys Asp Phe Thr Asp Lys Thr Tyr His Lys Ala Tyr Pro Thr Ile 
        115                 120                 125             


Asn His Leu Ile Lys Ala Trp Ile Glu Asn Lys Val Lys Pro Asp Pro 
    130                 135                 140                 


Arg Leu Leu Tyr Leu Ala Cys His Asn Ile Ile Lys Lys Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Glu Gly Asp Phe Asp Ser Glu Asn Gln Phe Asp Thr Ser 
                165                 170                 175     


Ile Gln Ala Leu Phe Glu Tyr Leu Arg Glu Asp Met Glu Val Asp Ile 
            180                 185                 190         


Asp Ala Asp Ser Gln Lys Val Lys Glu Ile Leu Lys Asp Ser Ser Leu 
        195                 200                 205             


Lys Asn Ser Glu Lys Gln Ser Arg Leu Asn Lys Ile Leu Gly Leu Lys 
    210                 215                 220                 


Pro Ser Asp Lys Gln Lys Lys Ala Ile Thr Asn Leu Ile Ser Gly Asn 
225                 230                 235                 240 


Lys Ile Asn Phe Ala Asp Leu Tyr Asp Asn Pro Asp Leu Lys Asp Ala 
                245                 250                 255     


Glu Lys Asn Ser Ile Ser Phe Ser Lys Asp Asp Phe Asp Ala Leu Ser 
            260                 265                 270         


Asp Asp Leu Ala Ser Ile Leu Gly Asp Ser Phe Glu Leu Leu Leu Lys 
        275                 280                 285             


Ala Lys Ala Val Tyr Asn Cys Ser Val Leu Ser Lys Val Ile Gly Asp 
    290                 295                 300                 


Glu Gln Tyr Leu Ser Phe Ala Lys Val Lys Ile Tyr Glu Lys His Lys 
305                 310                 315                 320 


Thr Asp Leu Thr Lys Leu Lys Ala Val Ile Lys Lys His Phe Pro Asn 
                325                 330                 335     


Asn Tyr Lys Lys Val Phe Gly Tyr Asn Gln Asp Asn Glu Ser Lys Asn 
            340                 345                 350         


Asn Tyr Ser Gly Tyr Val Gly Val Cys Lys Thr Lys Asn Lys Lys Leu 
        355                 360                 365             


Val Ile Asn Asn Ser Val Asn Gln Glu Asp Phe Tyr Lys Phe Leu Lys 
    370                 375                 380                 


Pro Ile Leu Ser Ala Lys Ser Glu Ile Lys Glu Val Asn Asp Ile Leu 
385                 390                 395                 400 


Ala Glu Ile Glu Thr Gly Asn Phe Leu Pro Lys Gln Ile Ser Lys Ser 
                405                 410                 415     


Asn Ala Glu Ile Pro Tyr Gln Leu Arg Lys Met Glu Leu Glu Lys Ile 
            420                 425                 430         


Leu Ser Asn Ala Glu Lys His Phe Ser Phe Leu Lys Gln Lys Asp Glu 
        435                 440                 445             


Lys Gly Leu Ser His Ser Glu Lys Ile Ile Met Leu Leu Thr Phe Lys 
    450                 455                 460                 


Ile Pro Tyr Tyr Ile Gly Pro Ile Asn Asp Asn His Lys Lys Ser Phe 
465                 470                 475                 480 


Pro Asp Arg Cys Trp Val Val Lys Lys Glu Lys Ser Pro Ser Gly Lys 
                485                 490                 495     


Thr Thr Pro Trp Asn Phe Phe Asp His Ile Asp Lys Glu Lys Thr Ala 
            500                 505                 510         


Glu Glu Phe Ile Thr Ser Arg Thr Asn Phe Cys Thr Tyr Leu Ile Gly 
        515                 520                 525             


Glu Ser Val Leu Pro Lys Asn Ser Leu Leu Tyr Ser Glu Tyr Thr Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Leu Gln Ile Ile Ile Asp Gly Lys Asn Ile 
545                 550                 555                 560 


Cys Asp Thr Lys Leu Lys Gln Lys Ile Tyr Glu Asp Leu Phe Lys Lys 
                565                 570                 575     


Tyr Lys Lys Ile Thr Gln Lys Gln Ile Ser Thr Phe Ile Lys His Glu 
            580                 585                 590         


Gly Ile Cys Asn Lys Thr Asp Glu Val Ile Ile Leu Gly Ile Asp Lys 
        595                 600                 605             


Glu Cys Thr Ser Ser Leu Lys Ser Tyr Ile Glu Leu Lys Asn Ile Phe 
    610                 615                 620                 


Gly Lys Gln Val Asp Glu Ile Ser Thr Lys Asn Met Leu Glu Glu Ile 
625                 630                 635                 640 


Ile Arg Trp Ala Thr Ile Tyr Asp Glu Gly Glu Gly Lys Thr Ile Leu 
                645                 650                 655     


Lys Thr Lys Ile Lys Ala Glu Tyr Gly Lys Tyr Cys Ser Asp Glu Gln 
            660                 665                 670         


Ile Lys Lys Ile Leu Asn Leu Lys Phe Ser Gly Trp Gly Arg Leu Ser 
        675                 680                 685             


Arg Lys Phe Leu Glu Thr Val Thr Ser Glu Met Pro Gly Phe Ser Glu 
    690                 695                 700                 


Pro Val Asn Ile Ile Thr Ala Met Arg Glu Thr Gln Asn Asn Leu Met 
705                 710                 715                 720 


Glu Leu Leu Ser Ser Glu Phe Lys Phe Thr Glu Asn Ile Lys Lys Ile 
                725                 730                 735     


Asn Ser Gly Phe Glu Asp Ala Glu Lys Gln Phe Ser Tyr Asp Gly Leu 
            740                 745                 750         


Val Lys Pro Leu Phe Leu Ser Pro Ser Val Lys Lys Met Leu Trp Gln 
        755                 760                 765             


Thr Leu Lys Leu Val Lys Glu Ile Ser His Ile Thr Gln Ala Pro Pro 
    770                 775                 780                 


Lys Lys Ile Phe Ile Glu Met Ala Lys Gly Ala Glu Leu Glu Pro Ala 
785                 790                 795                 800 


Arg Thr Lys Thr Arg Leu Lys Ile Leu Gln Asp Leu Tyr Ser Ser Cys 
                805                 810                 815     


Lys Asn Asp Ala Asp Ala Phe Ser Ser Glu Ile Gln Tyr Leu Ser Gly 
            820                 825                 830         


Lys Ile Glu Asn Glu Asp Asn Leu Arg Leu Arg Ser Asp Lys Leu Tyr 
        835                 840                 845             


Leu Tyr Tyr Thr Gln Leu Gly Lys Cys Met Tyr Cys Gly Lys Pro Ile 
    850                 855                 860                 


Glu Ile Gly His Val Phe Asp Thr Ser Asn Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile Ser Asn Arg Val Leu 
                885                 890                 895     


Val Cys Ser Ser Cys Asn Lys Asn Lys Glu Asp Lys Tyr Pro Leu Lys 
            900                 905                 910         


Ser Glu Ile Gln Ser Lys Gln Arg Gly Phe Trp Asn Phe Leu Gln Arg 
        915                 920                 925             


Asn Asn Phe Ile Ser Leu Glu Lys Leu Asn Arg Leu Thr Arg Thr Thr 
    930                 935                 940                 


Pro Ile Ser Asp Asp Glu Thr Ala Lys Phe Ile Ala Arg Gln Leu Val 
945                 950                 955                 960 


Glu Thr Arg Gln Ala Thr Lys Val Ala Ala Lys Val Leu Glu Lys Met 
                965                 970                 975     


Phe Pro Glu Thr Lys Ile Val Tyr Ser Lys Ala Glu Thr Val Ser Met 
            980                 985                 990         


Phe Arg Asn Lys Phe Asp Ile Ile  Lys Cys Arg Glu Ile  Asn Asp Phe 
        995                 1000                 1005             


His His  Ala His Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val 
    1010                 1015                 1020             


Tyr Asn  Thr Lys Phe Thr Asn  Asn Pro Trp Asn Phe  Ile Lys Glu 
    1025                 1030                 1035             


Lys Arg  Asp Asn Pro Lys Ile  Ala Asp Thr Tyr Asn  Tyr Tyr Lys 
    1040                 1045                 1050             


Val Phe  Asp Tyr Asp Val Lys  Arg Asn Asn Ile Thr  Ala Trp Glu 
    1055                 1060                 1065             


Lys Gly  Lys Thr Ile Ile Thr  Val Lys Asp Met Leu  Lys Arg Asn 
    1070                 1075                 1080             


Thr Pro  Ile Tyr Thr Arg Gln  Ala Ala Cys Lys Lys  Gly Glu Leu 
    1085                 1090                 1095             


Phe Asn  Gln Thr Ile Met Lys  Lys Gly Leu Gly Gln  His Pro Leu 
    1100                 1105                 1110             


Lys Lys  Glu Gly Pro Phe Ser  Asn Ile Ser Lys Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Asn Lys  Val Ser Ala Ala Tyr  Tyr Thr Leu Ile Glu  Tyr Glu Glu 
    1130                 1135                 1140             


Lys Val  Asn Lys Ile Arg Ser  Leu Glu Thr Ile Pro  Leu Tyr Leu 
    1145                 1150                 1155             


Val Lys  Asp Ile Gln Lys Asp  Gln Asp Val Leu Lys  Ser Tyr Leu 
    1160                 1165                 1170             


Thr Asp  Leu Leu Gly Lys Lys  Glu Ile Lys Ile Leu  Val Pro Lys 
    1175                 1180                 1185             


Ile Lys  Ile Asn Ser Leu Leu  Lys Ile Asn Gly Phe  Pro Cys His 
    1190                 1195                 1200             


Ile Thr  Gly Lys Thr Gly Asp  Ser Phe Val Leu Arg  Pro Ala Val 
    1205                 1210                 1215             


Gln Phe  Cys Cys Ser Asn Asp  Glu Val Leu Tyr Phe  Lys Lys Ile 
    1220                 1225                 1230             


Ile Arg  Phe Asn Glu Ile Arg  Ser Gln Arg Glu Lys  Met Gly Lys 
    1235                 1240                 1245             


Thr Ile  Ser Pro Tyr Glu Asp  Leu Ser Phe Arg Ser  Tyr Ile Lys 
    1250                 1255                 1260             


Glu Asn  Leu Cys Lys Lys Thr  Lys Ala Asp Glu Ile  Gly Glu Lys 
    1265                 1270                 1275             


Glu Phe  Tyr Asp Leu Leu Gln  Lys Lys Asn Leu Glu  Ile Tyr Asp 
    1280                 1285                 1290             


Met Leu  Leu Thr Lys His Lys  Asp Thr Ile Tyr Asn  Lys Arg Pro 
    1295                 1300                 1305             


Asn Ser  Thr Thr Leu Gly Met  Leu Thr Lys Gly Arg  Tyr Glu Phe 
    1310                 1315                 1320             


Ile Asn  Leu Lys Pro Lys Asp  Gln Ile Ile Val Met  Leu Glu Ile 
    1325                 1330                 1335             


Leu Lys  Leu Phe Cys Thr Thr  Arg Glu Ala Ile Asp  Leu Gly Leu 
    1340                 1345                 1350             


Ile Lys  Gly Lys Pro Ala Ala  Gly Val Ala Thr Leu  Gly Lys Lys 
    1355                 1360                 1365             


Ile Ser  Asn Leu Asp Asn Cys  Ile Leu Ile Tyr Gln  Ser Ile Thr 
    1370                 1375                 1380             


Gly Ile  Phe Glu Lys Arg Ile  Asp Leu Leu Lys Val  
    1385                 1390                 1395 


<210>  1162
<211>  1395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1162

Met Lys Lys Glu Ile Lys Asp Tyr Phe Leu Gly Leu Asp Val Gly Thr 
1               5                   10                  15      


Gly Ser Val Gly Trp Ala Val Thr Asp Thr Asp Tyr Lys Leu Leu Lys 
            20                  25                  30          


Ala Asn Arg Lys Asp Leu Trp Gly Met Arg Cys Phe Glu Thr Ala Glu 
        35                  40                  45              


Thr Ala Glu Val Arg Arg Leu His Arg Gly Ala Arg Arg Arg Ile Glu 
    50                  55                  60                  


Arg Arg Lys Lys Arg Ile Lys Leu Leu Gln Glu Leu Phe Ser Gln Glu 
65                  70                  75                  80  


Ile Ala Lys Ile Asp Glu Gly Phe Phe Gln Arg Met Lys Glu Ser Pro 
                85                  90                  95      


Leu Tyr Ala Glu Asp Lys Thr Ile Leu Gln Glu Asn Thr Leu Phe Asn 
            100                 105                 110         


Asp Lys Asp Phe Thr Asp Lys Thr Tyr His Lys Ala Tyr Pro Thr Ile 
        115                 120                 125             


Asn His Leu Ile Lys Ala Trp Ile Glu Asn Lys Val Lys Pro Asp Pro 
    130                 135                 140                 


Arg Leu Leu Tyr Leu Ala Cys His Asn Ile Ile Lys Lys Arg Gly His 
145                 150                 155                 160 


Phe Leu Phe Glu Gly Asp Phe Asp Ser Glu Asn Gln Phe Asp Thr Ser 
                165                 170                 175     


Ile Gln Ala Leu Phe Glu Tyr Leu Arg Glu Asp Met Glu Val Asp Ile 
            180                 185                 190         


Asp Ala Asp Ser Gln Lys Val Lys Glu Ile Leu Lys Asp Ser Ser Leu 
        195                 200                 205             


Lys Asn Ser Glu Lys Gln Ser Arg Leu Asn Lys Met Leu Gly Leu Lys 
    210                 215                 220                 


Pro Ser Asp Lys Gln Lys Lys Ala Ile Thr Asn Leu Ile Ser Gly Asn 
225                 230                 235                 240 


Lys Ile Asn Phe Ala Asp Leu Tyr Asp Asn Pro Asp Leu Lys Asp Ala 
                245                 250                 255     


Glu Lys Asn Ser Ile Ser Phe Ser Lys Asp Asp Phe Asp Ala Leu Ser 
            260                 265                 270         


Asp Asp Leu Ala Ser Ile Leu Gly Asp Ser Phe Glu Leu Leu Leu Lys 
        275                 280                 285             


Ala Lys Ala Val Tyr Asn Cys Ser Val Leu Ser Lys Val Ile Gly Asp 
    290                 295                 300                 


Glu Gln Tyr Leu Ser Phe Ala Lys Val Lys Ile Tyr Glu Lys His Lys 
305                 310                 315                 320 


Thr Asp Leu Thr Lys Leu Lys Asn Val Ile Lys Lys His Phe Pro Lys 
                325                 330                 335     


Asp Tyr Lys Lys Val Phe Gly Tyr Asn Lys Asn Glu Lys Asn Asn Asn 
            340                 345                 350         


Asn Tyr Ser Gly Tyr Val Gly Val Cys Lys Thr Lys Ser Lys Lys Leu 
        355                 360                 365             


Ile Ile Asn Asn Ser Val Asn Gln Glu Asp Phe Tyr Lys Phe Leu Lys 
    370                 375                 380                 


Thr Ile Leu Ser Ala Lys Ser Glu Ile Lys Glu Val Asn Asp Ile Leu 
385                 390                 395                 400 


Thr Glu Ile Glu Thr Gly Thr Phe Leu Pro Lys Gln Ile Ser Lys Ser 
                405                 410                 415     


Asn Ala Glu Ile Pro Tyr Gln Leu Arg Lys Met Glu Leu Glu Lys Ile 
            420                 425                 430         


Leu Ser Asn Ala Glu Lys His Phe Ser Phe Leu Lys Gln Lys Asp Glu 
        435                 440                 445             


Lys Gly Leu Ser His Ser Glu Lys Ile Ile Met Leu Leu Thr Phe Lys 
    450                 455                 460                 


Ile Pro Tyr Tyr Ile Gly Pro Ile Asn Asp Asn His Lys Lys Ser Phe 
465                 470                 475                 480 


Pro Asp Arg Cys Trp Val Val Lys Lys Glu Lys Ser Pro Ser Gly Lys 
                485                 490                 495     


Thr Thr Pro Trp Asn Phe Phe Asp His Ile Asp Lys Glu Lys Thr Ala 
            500                 505                 510         


Glu Glu Phe Ile Thr Ser Arg Thr Asn Phe Cys Thr Tyr Leu Ile Gly 
        515                 520                 525             


Glu Ser Val Leu Pro Lys Asn Ser Leu Leu Tyr Ser Glu Tyr Thr Val 
    530                 535                 540                 


Leu Asn Glu Ile Asn Asn Leu Gln Ile Ile Ile Asp Gly Lys Asn Ile 
545                 550                 555                 560 


Cys Asp Thr Lys Leu Lys Gln Lys Ile Tyr Glu Glu Leu Phe Lys Lys 
                565                 570                 575     


Tyr Lys Lys Ile Thr Gln Lys Gln Ile Ser Thr Phe Ile Lys His Glu 
            580                 585                 590         


Gly Ile Cys Asn Lys Thr Asp Glu Val Ile Ile Leu Gly Ile Asp Lys 
        595                 600                 605             


Glu Cys Thr Ser Ser Leu Lys Ser Tyr Ile Glu Leu Lys Asn Ile Phe 
    610                 615                 620                 


Gly Lys Gln Val Asp Glu Ile Ser Thr Lys Asn Met Leu Glu Glu Ile 
625                 630                 635                 640 


Ile Arg Trp Ala Thr Ile Tyr Asp Glu Gly Glu Gly Lys Thr Ile Leu 
                645                 650                 655     


Lys Thr Lys Ile Lys Ala Glu Tyr Gly Lys Tyr Cys Ser Asp Glu Gln 
            660                 665                 670         


Ile Lys Lys Ile Leu Asn Leu Lys Phe Ser Gly Trp Gly Arg Leu Ser 
        675                 680                 685             


Arg Lys Phe Leu Glu Thr Val Thr Ser Glu Met Pro Gly Phe Ser Glu 
    690                 695                 700                 


Pro Val Asn Ile Ile Thr Ala Met Arg Glu Thr Gln Asn Asn Leu Met 
705                 710                 715                 720 


Glu Leu Leu Ser Ser Glu Phe Lys Phe Thr Glu Asn Ile Lys Lys Ile 
                725                 730                 735     


Asn Ser Gly Phe Glu Asp Val Glu Lys Gln Phe Ser Tyr Asp Gly Leu 
            740                 745                 750         


Val Lys Pro Leu Phe Leu Ser Pro Ser Val Lys Lys Met Leu Trp Gln 
        755                 760                 765             


Thr Leu Lys Leu Val Lys Glu Ile Ser His Ile Thr Gln Ala Pro Pro 
    770                 775                 780                 


Lys Lys Ile Phe Ile Glu Met Ala Lys Gly Ala Glu Leu Asp Pro Val 
785                 790                 795                 800 


Arg Thr Lys Thr Arg Leu Lys Ile Leu Gln Asp Leu Tyr Asn Asn Cys 
                805                 810                 815     


Lys Asn Asp Ala Asp Ala Phe Ser Ser Glu Ile Lys Asp Leu Ser Gly 
            820                 825                 830         


Lys Ile Glu Asn Glu Asp Asn Leu Arg Leu Arg Ser Asp Lys Leu Tyr 
        835                 840                 845             


Leu Tyr Tyr Thr Gln Leu Gly Lys Cys Met Tyr Cys Gly Lys Pro Ile 
    850                 855                 860                 


Glu Ile Gly His Val Phe Asp Thr Ser Asn Tyr Asp Ile Asp His Ile 
865                 870                 875                 880 


Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile Ser Asn Arg Val Leu 
                885                 890                 895     


Val Cys Ser Ser Cys Asn Lys Asn Lys Glu Asp Lys Tyr Pro Leu Lys 
            900                 905                 910         


Ala Glu Ile Gln Ser Lys Gln Arg Gly Phe Trp Asn Phe Leu Gln Arg 
        915                 920                 925             


Asn Asn Phe Ile Ser Leu Glu Lys Phe Asn Arg Leu Thr Arg Ala Thr 
    930                 935                 940                 


Pro Ile Ser Asp Asp Glu Thr Ala Lys Phe Ile Ala Arg Gln Leu Val 
945                 950                 955                 960 


Glu Thr Arg Gln Ala Thr Lys Val Ala Ala Arg Val Leu Glu Lys Met 
                965                 970                 975     


Phe Pro Glu Thr Lys Ile Val Tyr Ser Lys Ala Glu Thr Val Ser Met 
            980                 985                 990         


Phe Arg Asn Lys Phe Asp Ile Val  Lys Cys Arg Glu Ile  Asn Asp Phe 
        995                 1000                 1005             


His His  Ala His Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val 
    1010                 1015                 1020             


Tyr Asn  Thr Lys Phe Thr Asn  Asn Leu Trp Asn Phe  Ile Lys Glu 
    1025                 1030                 1035             


Lys Arg  Asp Asn Pro Lys Ile  Ala Asp Thr Tyr Asn  Tyr Tyr Lys 
    1040                 1045                 1050             


Val Phe  Asp Tyr Asp Val Lys  Arg Asn Asn Ile Thr  Ala Trp Glu 
    1055                 1060                 1065             


Lys Gly  Lys Thr Ile Ile Thr  Val Lys Asp Met Leu  Lys Arg Asn 
    1070                 1075                 1080             


Thr Pro  Ile Tyr Thr Arg Gln  Ala Ala Cys Lys Lys  Gly Gly Leu 
    1085                 1090                 1095             


Phe Asp  Gln Thr Ile Met Lys  Lys Gly Leu Gly Gln  His Pro Leu 
    1100                 1105                 1110             


Lys Lys  Glu Gly Pro Phe Ser  Asn Ile Ser Lys Tyr  Gly Gly Tyr 
    1115                 1120                 1125             


Asn Lys  Val Ser Ala Ala Tyr  Tyr Thr Leu Ile Glu  Tyr Glu Glu 
    1130                 1135                 1140             


Lys Gly  Asn Lys Ile Arg Ser  Leu Glu Thr Ile Pro  Leu Tyr Leu 
    1145                 1150                 1155             


Val Lys  Asp Ile Gln Lys Asp  Gln Asp Val Leu Lys  Ser Tyr Leu 
    1160                 1165                 1170             


Thr Asp  Leu Leu Gly Lys Lys  Glu Phe Lys Ile Leu  Val Pro Lys 
    1175                 1180                 1185             


Ile Lys  Ile Asn Ser Leu Leu  Lys Ile Asn Gly Phe  Pro Cys His 
    1190                 1195                 1200             


Ile Thr  Gly Lys Thr Asn Asp  Ser Phe Leu Leu Arg  Pro Ala Val 
    1205                 1210                 1215             


Gln Phe  Cys Cys Ser Asn Asp  Glu Val Leu Tyr Phe  Lys Lys Ile 
    1220                 1225                 1230             


Ile Arg  Phe Ser Glu Ile Arg  Ser Gln Arg Glu Lys  Ile Gly Lys 
    1235                 1240                 1245             


Thr Ile  Ser Pro Tyr Glu Asp  Leu Ser Phe Arg Ser  Tyr Ile Lys 
    1250                 1255                 1260             


Glu Asn  Leu Cys Lys Lys Thr  Lys Asn Asp Glu Ile  Gly Glu Lys 
    1265                 1270                 1275             


Glu Phe  Tyr Asp Leu Leu Gln  Lys Lys Asn Leu Glu  Ile Tyr Asp 
    1280                 1285                 1290             


Met Leu  Leu Thr Lys His Lys  Asp Thr Ile Tyr Lys  Lys Arg Pro 
    1295                 1300                 1305             


Asn Ser  Ala Thr Ile Asp Ile  Leu Val Lys Gly Lys  Glu Lys Phe 
    1310                 1315                 1320             


Lys Ser  Leu Ile Ile Glu Asn  Gln Phe Glu Val Ile  Leu Glu Ile 
    1325                 1330                 1335             


Leu Lys  Leu Phe Ser Ala Thr  Arg Asn Val Ser Asp  Leu Gln His 
    1340                 1345                 1350             


Ile Gly  Gly Ser Lys Tyr Ser  Gly Val Ala Lys Ile  Gly Asn Lys 
    1355                 1360                 1365             


Ile Ser  Ser Leu Asp Asn Cys  Ile Leu Ile Tyr Gln  Ser Ile Thr 
    1370                 1375                 1380             


Gly Ile  Phe Glu Lys Arg Ile  Asp Leu Leu Lys Val  
    1385                 1390                 1395 


<210>  1163
<211>  1396
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1163

Met Asp Ser Ser Lys Gln Gly Leu Arg Arg Tyr His Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Thr Asn Ser Ile Gly Trp Thr Ala Ile Asp Asp His Phe Ser 
            20                  25                  30          


Leu Leu Arg Val Lys Gly Lys Asn Ala Ile Gly Val Arg Thr Phe Lys 
        35                  40                  45              


Glu Gly Glu Thr Ala Ala Asp Arg Arg Gly Phe Arg Thr Ala Arg Arg 
    50                  55                  60                  


Arg Leu Ser Arg Arg Arg Trp Arg Leu Gln Phe Leu Asp Glu Phe Phe 
65                  70                  75                  80  


Ala Pro Tyr Leu Ala Glu Val Asp Pro Asn Phe Leu Ala Arg Leu Lys 
                85                  90                  95      


Gln Ser Asp Ile Ser Ala Lys Asp Pro Ala Lys Asn Gln Glu Tyr Met 
            100                 105                 110         


Gly Lys Leu Leu Phe Pro Asp Gln Glu Ala Ala Ser Asp Gly Asn Gly 
        115                 120                 125             


Tyr Pro Thr Leu Ile Gln Met Arg Lys Ala Met Pro Ala Glu Lys Ala 
    130                 135                 140                 


Ala Asp Phe Pro Val Phe Asn Ile Tyr Gln Leu Arg Tyr Ala Leu Met 
145                 150                 155                 160 


Asn Glu Lys Arg Lys Phe Asp Leu Arg Glu Ile Tyr Leu Ala Val His 
                165                 170                 175     


His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asn Asn Thr Ser Val Asp 
            180                 185                 190         


Lys Phe Lys Ala Asp Gln Ile Asp Phe Asn Lys Ser Phe Glu Thr Leu 
        195                 200                 205             


Asn Asp Leu Phe Arg Gln Val Gln Pro Ile Ala Pro Phe Gln Ile Asp 
    210                 215                 220                 


Leu Thr Lys Val Asp Glu Leu Gly Lys Leu Leu Leu Asp Thr Lys Gln 
225                 230                 235                 240 


Lys Lys Leu Asp Arg Gln Lys Ala Val Ala Lys Leu Leu Pro Leu Ile 
                245                 250                 255     


Glu Asp Gly Thr Asp Lys Glu Ile Val Lys Ala His Gln Gln Thr Ala 
            260                 265                 270         


Arg Glu Ile Ser Lys Leu Ile Leu Gly Tyr Lys Ala Lys Val Gly Leu 
        275                 280                 285             


Ile Leu Gln Asn Asn Leu Asp Gln Thr Leu Asp Met Ser Thr Glu Asn 
    290                 295                 300                 


Ser Asp Asp Gln Leu Met Gln Ile Ala Glu Glu Leu Asp Asp Tyr Gln 
305                 310                 315                 320 


Met Glu Leu Ile Asn Gln Leu Ser Leu Leu Tyr Ser Gln Ile Met Leu 
                325                 330                 335     


Asn Glu Ile Val Pro Asn Gly Glu Thr Val Ser Ala Ser Met Leu Asn 
            340                 345                 350         


Arg Tyr Tyr Lys His Arg Cys Gln Leu Lys Glu Leu Lys Asp Tyr Gly 
        355                 360                 365             


Ala Ala Gln Asp Lys Lys Thr Arg Glu Gln Leu Asp His Leu Tyr Ala 
    370                 375                 380                 


Glu Tyr Ile Gly Gln Ile Pro Lys Asp Ser Lys Phe Asp Phe Thr Lys 
385                 390                 395                 400 


Asp Leu Lys Lys Leu Met Asp Lys Ser Asp Leu Gly Gln Lys Ile Lys 
                405                 410                 415     


Asp Glu Ile Glu Ala Gly Asp Tyr Leu Pro Lys Gln Arg Thr Ser Ala 
            420                 425                 430         


Asn Gly Val Ile Pro His Gln Met His Gln Gln Glu Leu Asp Gln Ile 
        435                 440                 445             


Ile Glu Asn Gln Lys Glu Tyr Tyr Pro Trp Leu Ala Glu Pro Asn Pro 
    450                 455                 460                 


Val Glu Lys Asn Ile Lys Asn Ala Lys Tyr Lys Leu Asp Gln Leu Val 
465                 470                 475                 480 


Ser Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Leu Ala Asp 
                485                 490                 495     


Gln Gln Lys Thr Ser Lys Ala Ser Phe Ala Trp Ala Val Arg Lys Lys 
            500                 505                 510         


Ala Gly Lys Ile Thr Pro Trp Asn Phe Tyr Asp Met Ile Asp Arg Glu 
        515                 520                 525             


Lys Ser Ala Asn Asn Phe Ile Lys Arg Met Thr Asn Lys Asp Thr Tyr 
    530                 535                 540                 


Leu Leu Ser Glu Asp Val Leu Pro Ala Ser Ser Leu Leu Tyr Gln Lys 
545                 550                 555                 560 


Phe Thr Val Leu Asn Glu Leu Asn Asn Ile Arg Val Asn Gly Arg Lys 
                565                 570                 575     


Leu Gln Pro Ala Leu Lys Gln Ala Ile Tyr Thr Asp Leu Phe Gln Lys 
            580                 585                 590         


Arg Lys Thr Val Asn Leu Lys Thr Leu Thr Asn Phe Leu Ala Ile His 
        595                 600                 605             


Glu Pro Thr Leu Thr Lys Pro Lys Ile Glu Gly Leu Ser Asn Gly Arg 
    610                 615                 620                 


Ser Phe Asn Ser Ser Leu Gly Thr Tyr Ile Asp Met Ser Lys Ile Leu 
625                 630                 635                 640 


Gly Asp Ser Leu Asp Asp Leu Asn Tyr Arg Asp Asp Ile Glu Lys Ile 
                645                 650                 655     


Ile Glu Trp Ser Thr Val Phe Glu Asp Ser Glu Ile Tyr Gly Val Lys 
            660                 665                 670         


Leu Gln Glu Ile Gln Trp Leu Thr Asn Glu Gln Arg Glu Lys Leu Val 
        675                 680                 685             


Arg Lys Arg Tyr Gln Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Thr 
    690                 695                 700                 


Gly Ile Thr Asp Glu Asn Gly Gln Arg Ile Ile Asp Leu Met Trp Asp 
705                 710                 715                 720 


Thr Ser Lys Asn Phe Met Gln Ile Val His Gln Pro Val Phe Glu Glu 
                725                 730                 735     


Gln Ile Ala Glu Leu Asn Gln Gln Leu Leu Gln Asp Ala Asp Lys Ala 
            740                 745                 750         


Pro Leu Glu Val Val Asp Asp Ile Leu Ala Asp Ala Tyr Thr Ser Pro 
        755                 760                 765             


Gln Asn Lys Lys Ala Ile Arg Gln Val Val Lys Val Val Ala Asp Val 
    770                 775                 780                 


Val Lys Ala Val Gly Asn Ala Pro Ala Ser Ile Ser Ile Glu Phe Ala 
785                 790                 795                 800 


Arg Asn Glu Asp Arg Asn Lys Glu Leu Lys Asn Ser Arg Arg His Ser 
                805                 810                 815     


Ile Glu Lys Leu Tyr His Glu Thr Ala Glu Lys Leu Val Lys Gln Thr 
            820                 825                 830         


Asn Leu Leu Glu Glu Leu Gly Asn Val Lys Asp Leu Asn Asp Arg Tyr 
        835                 840                 845             


Tyr Leu Tyr Phe Thr Gln Ala Gly Arg Asp Met Tyr Asp Gly Thr Pro 
    850                 855                 860                 


Ile Asn Ile Asp Glu Ile Ser Thr His Tyr Glu Ile Asp His Ile Leu 
865                 870                 875                 880 


Pro Gln Ser Phe Leu Lys Asp Asn Ser Leu Asp Asn Arg Val Leu Val 
                885                 890                 895     


Lys Lys Ser Glu Asn Ala Ala Lys Ser Asp Arg Val Pro Ala Lys Leu 
            900                 905                 910         


Tyr Gly Ala Lys Met Arg Ala Phe Trp Lys Gln Leu Lys Glu Ser Gln 
        915                 920                 925             


Leu Ile Ser Asn Arg Lys Tyr Leu Asn Leu Thr Thr Asp Pro Glu Gln 
    930                 935                 940                 


Val Asp Lys Tyr Gln Met Gln Gly Phe Val His Arg Gln Leu Val Glu 
945                 950                 955                 960 


Thr Arg Gln Ile Ile Lys Leu Ala Ala Asn Ile Phe Gly Ser Leu Tyr 
                965                 970                 975     


Pro Asp Ser Ala Val Ile Glu Thr Arg Ala Glu Leu Thr Lys Gln Leu 
            980                 985                 990         


Arg Glu Lys Phe Ser Leu Tyr Lys  Val Arg Asp Val Asn  Asp Tyr His 
        995                 1000                 1005             


His Ala  Val Asp Ala Tyr Leu  Thr Ala Phe Ala Gly  His Tyr Leu 
    1010                 1015                 1020             


Tyr Gln  Arg Tyr Pro Lys Leu  Arg Pro Met Phe Val  Tyr Gly Asp 
    1025                 1030                 1035             


Tyr Ala  Lys Ile Tyr Ala Asp  Asp Leu Lys Arg Leu  Arg Ser Val 
    1040                 1045                 1050             


Asn Leu  Phe His Asp Leu Glu  Lys Asp Gln Tyr Asn  Ser Gly Thr 
    1055                 1060                 1065             


Ala Val  Asn Pro Glu Thr Lys  Glu Lys Ile Val Asp  Val Asp Trp 
    1070                 1075                 1080             


Leu Ser  Asp Tyr Val Ser Lys  Ile Gln His Phe Lys  Tyr Met Leu 
    1085                 1090                 1095             


Ile Ser  Lys Ala Val Tyr Thr  Gln His Gly Gln Leu  Tyr Gly Ala 
    1100                 1105                 1110             


Thr Ile  Leu Pro Val Ala Gln  Val Asp Lys Ile Ser  Ala Pro Ile 
    1115                 1120                 1125             


Gly Ile  Lys Asp Asp Lys Pro  Val Asp Ile Tyr Gly  Ala Tyr Thr 
    1130                 1135                 1140             


Asn Glu  Val Ala Ala Tyr Met  Ala Ile Val Arg Val  Asn Gly Lys 
    1145                 1150                 1155             


Lys Pro  Lys Tyr Lys Val Val  Ser Val Pro Val Arg  Met Leu Gln 
    1160                 1165                 1170             


Arg Leu  Lys Met Ala Glu Gln  Lys Gly His Asp Gln  Tyr Met Gln 
    1175                 1180                 1185             


Thr Leu  His Asp Val Leu Leu  Arg Asn Phe Thr Ser  Leu Lys Lys 
    1190                 1195                 1200             


Asn Arg  Lys Thr Gly Leu Lys  Glu Ala Val Val Lys  Asp Phe Asp 
    1205                 1210                 1215             


Val Leu  Val Pro Cys Val Arg  Tyr Asn Gln Leu Val  Ile Asp Gly 
    1220                 1225                 1230             


Asp Thr  Lys Phe Thr Leu Gly  Ser Ala Thr Tyr Gln  His Asn Ala 
    1235                 1240                 1245             


Lys Gln  Leu Val Leu Cys Asn  His Ser Leu Glu Thr  Leu Ala Asn 
    1250                 1255                 1260             


Asn Phe  Glu Tyr Val Arg Lys  His Pro Asp Glu Ala  Asp Glu Arg 
    1265                 1270                 1275             


Met Ile  Met Leu Tyr Asp Asp  Ile Leu Asp Gln Val  Asn His Tyr 
    1280                 1285                 1290             


Phe Thr  Leu Tyr Asp Lys Asn  Lys Phe Arg Glu Lys  Leu Asn Ala 
    1295                 1300                 1305             


Gly Arg  Asp Lys Tyr Leu Lys  Leu Pro Thr Phe Ser  Gln Phe Glu 
    1310                 1315                 1320             


Asn Asn  Lys Lys Thr Ala Ile  Gly Lys Ser Glu Ala  Ile Ile Asn 
    1325                 1330                 1335             


Ile Leu  Arg Gly Leu His Ala  Asn Ala Ala Ile Thr  Asn Leu Lys 
    1340                 1345                 1350             


Cys Leu  Gly Val Ser Thr Pro  Phe Gly Gln Leu Gln  Ser Pro Ser 
    1355                 1360                 1365             


Gly Ile  Glu Leu Ser Glu Asn  Ala Lys Leu Val Tyr  Gln Ser Pro 
    1370                 1375                 1380             


Thr Gly  Leu Phe Ser Arg Glu  Val Leu Leu Lys Asp  Leu 
    1385                 1390                 1395     


<210>  1164
<211>  1396
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1164

Met Ala Asn Leu Arg Asn Tyr Lys Gly Asp Tyr Asn Ile Gly Leu Asp 
1               5                   10                  15      


Met Gly Thr Gly Ser Val Gly Trp Ala Val Thr Asp Ser Glu Gly Lys 
            20                  25                  30          


Leu Leu His Phe Lys Lys Gln Pro Thr Trp Gly Ser Arg Leu Phe Asp 
        35                  40                  45              


Ser Ala Glu Thr Ala Ala Ser Ala Arg Ile Pro Arg Gly Gln Arg Arg 
    50                  55                  60                  


Arg Tyr Val Arg Arg Arg Trp Arg Leu Asp Leu Leu Gln Gln Leu Phe 
65                  70                  75                  80  


Gln Asp Glu Val Ser Lys Ala Asp Pro Glu Phe Phe Ile Arg Leu Arg 
                85                  90                  95      


Gln Ser Arg Leu Leu Lys Glu Asp Arg Glu Glu Gly His Arg Asp Tyr 
            100                 105                 110         


Asn His Pro Leu Phe Asn Asp Ser Asp Phe Thr Glu Thr Asp Tyr Tyr 
        115                 120                 125             


Asn Arg Phe Pro Thr Ile Tyr His Leu Arg Lys Trp Leu Met Glu Thr 
    130                 135                 140                 


Asp Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Ala His Asn Ile 
145                 150                 155                 160 


Val Lys His Arg Gly Asn Phe Leu Arg Gln Glu Asn Thr Ser Leu Ser 
                165                 170                 175     


Ala Lys Asn Ala Arg Pro Leu Asp Ala Leu Arg Glu Phe Arg Ser Ala 
            180                 185                 190         


Leu Val Asp Trp Cys Ala Glu Leu Gly Tyr Glu Cys Asn Pro Asp Glu 
        195                 200                 205             


Ser Gly Glu Ile Ser Asn Glu Ile Leu Ser Val Leu Glu Glu Glu His 
    210                 215                 220                 


Val Ala Asn Ser Asp Lys Lys Lys Arg Ile Ser Ser Leu Ile Gly Val 
225                 230                 235                 240 


Ala Met Asn Glu Gly Ala Lys Glu Ser Lys Ala Cys Asn Asp Ala Leu 
                245                 250                 255     


Ala Gly Ala Met Val Gly Leu Lys Ala Glu Phe Lys Asn Val Phe Gly 
            260                 265                 270         


Glu Ile Ser Ala Glu Gln Thr Ser Ile Tyr Leu Ser Asn Asp Glu Ala 
        275                 280                 285             


Val Asp Ala Leu Arg Glu Gly Ile Pro Asp Glu Gly Leu Glu Leu Phe 
    290                 295                 300                 


Gly Lys Leu Cys Ser Val Tyr Ser Ala Tyr Val Leu Gln Gly Leu Leu 
305                 310                 315                 320 


Ser Tyr Ala Pro Gly Gln Thr Ile Ser Val Asn Met Val Ala Lys Tyr 
                325                 330                 335     


Glu Lys Tyr Gly Asn Asp Leu Lys Thr Leu Lys Ala Leu Val Cys Glu 
            340                 345                 350         


Tyr Ala Arg Ala Glu Tyr Asp Ser Phe Phe Arg Gly Glu Phe Tyr Arg 
        355                 360                 365             


Asp Gln Asp Gly His Glu Leu Asn Ile Tyr Asn Ala Ser Lys Ala Gln 
    370                 375                 380                 


Gly Tyr Thr Arg Tyr Asn Leu Gly Thr Ser Lys Met Ser Tyr Glu Asp 
385                 390                 395                 400 


Phe Glu Lys Ala Val Lys Lys Leu Phe Ala Gly Thr Ala Ala Glu Glu 
                405                 410                 415     


Asp Gly Arg Tyr Leu Gln Met Met Lys Glu Phe Gly Glu Gln Arg Phe 
            420                 425                 430         


Leu Arg Arg Leu Lys Thr Ser Asp Asn Gly Ser Ile Tyr Tyr Gln Leu 
        435                 440                 445             


His Leu Glu Glu Leu Thr Ala Ile Leu Ser Asn Gln Gly Lys His Tyr 
    450                 455                 460                 


Pro Phe Leu Val Glu Asp Ala Glu Lys Ile Lys Ser Leu Val Ser Phe 
465                 470                 475                 480 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Thr Asp Arg Asn Ala Arg Lys 
                485                 490                 495     


Asp Lys His Gly Lys Asn Arg Phe Ala Trp Ser Glu Arg Lys Ser Gly 
            500                 505                 510         


Met Glu Asp Ala Val Ile Thr Pro Trp Asn Trp Asp Glu Val Ile Asp 
        515                 520                 525             


Arg Gly Lys Ser Ala Glu Ala Phe Ile Thr Arg Met Thr Gly Met Cys 
    530                 535                 540                 


Thr Tyr Leu Gln Gly Glu Asp Val Leu Pro Lys Cys Ser Leu Leu Tyr 
545                 550                 555                 560 


Glu Glu Phe Cys Val Leu Asn Glu Leu Asn Gly Ser Arg Trp Gly Met 
                565                 570                 575     


Asp Gly Asp Lys Glu His Arg Phe Asp Ala Ala Asp Arg Glu Ala Ile 
            580                 585                 590         


Ile Glu Glu Leu Phe Lys Lys Lys Lys Thr Val Lys Tyr Lys Asp Val 
        595                 600                 605             


Ala Asn Trp Leu Glu Arg Glu Ala Gly Arg Met Asn Val His Val Met 
    610                 615                 620                 


Gly Gly Gln Gly Glu Thr Gly Tyr Glu Ser Lys Leu Ser Ser Tyr Tyr 
625                 630                 635                 640 


Phe Phe Arg Arg Asp Ile Phe Asp Val Glu Glu Leu Asp Arg Ala Asp 
                645                 650                 655     


Tyr Pro Met Ile Glu Gln Ile Ile Leu Trp Asn Thr Leu Phe Glu Asp 
            660                 665                 670         


Arg Arg Ile Leu Lys Glu Lys Leu Glu Asp Ala Tyr Gly Pro Lys Gly 
        675                 680                 685             


Asn Gly Arg Leu Asp Asp Asp Gln Ile Lys Lys Ile Cys Arg Lys Arg 
    690                 695                 700                 


Phe Thr Gly Trp Gly Arg Leu Ser Lys Lys Phe Leu Thr Gly Ile Lys 
705                 710                 715                 720 


Val Asp Thr Asp Cys Gly Pro Arg Ser Ile Met Asp Val Leu Arg Glu 
                725                 730                 735     


Gly Asp Pro Asn Ala Gly Phe Arg Gly Lys Thr Met Val Met Met Glu 
            740                 745                 750         


Val Leu Arg Asp Glu Asn Leu Gly Phe Gln Gln Lys Val Asp Glu Phe 
        755                 760                 765             


Asn Lys Asn Tyr Phe Gly Gln Lys Gly Lys Ala Leu Glu Val Asn Glu 
    770                 775                 780                 


Leu Pro Gly Ser Pro Ala Ile Arg Arg Ser Leu Asn Gln Ala Ile Arg 
785                 790                 795                 800 


Ile Val Asp Glu Ile Ala Lys Ile Ala Gly Lys Asp Pro Glu Ser Ile 
                805                 810                 815     


Phe Ile Glu Val Thr Arg Asp Asp Glu Leu Asp Lys Arg Gly Lys Arg 
            820                 825                 830         


Thr Lys Arg Arg Tyr Asp Ala Leu Lys Glu Ala Leu Thr Val Phe Lys 
        835                 840                 845             


Lys Glu Asp Pro Glu Ile Trp Ala Glu Leu Gln Ala Thr Ser Pro Ser 
    850                 855                 860                 


Asp Leu Asp Glu Arg Leu Thr Leu Tyr Phe Met Gln Arg Gly Lys Cys 
865                 870                 875                 880 


Leu Tyr Ser Gly Glu Pro Ile Asp Ile Asn Lys Leu Ser Ser Gly Glu 
                885                 890                 895     


Tyr Glu Val Asp His Ile Ile Pro Arg Ser Tyr Ile Lys Asp Asp Ser 
            900                 905                 910         


Leu Glu Asn Lys Ala Leu Val Cys Arg Lys Tyr Asn Gln Ser Lys Thr 
        915                 920                 925             


Asp Ala Met Leu Leu Asp Pro Ser Ile Arg Arg Lys Met Arg Ser Tyr 
    930                 935                 940                 


Trp Asp Met Leu Leu Asp Ala Lys Leu Ile Gly Pro Lys Lys His Arg 
945                 950                 955                 960 


Asn Leu Leu Arg Asp His Ile Asp Glu Lys Ala Met Lys Gly Phe Val 
                965                 970                 975     


Ala Arg Gln Leu Val Glu Thr Ser Gln Ser Val Lys Leu Ala Gly Ser 
            980                 985                 990         


Leu Leu Ser Val Arg Tyr Pro Asn  Ala Asn Ile Val Pro  Val Lys Ala 
        995                 1000                 1005             


Ser Met  Ser His Asp Leu Arg  Glu Ala Ala Gly Phe  Val Lys Cys 
    1010                 1015                 1020             


Arg Glu  Ala Asn Asp Phe His  His Ala His Asp Ala  Tyr Leu Ala 
    1025                 1030                 1035             


Cys Arg  Met Gly Leu Phe Ile  Gln Met Arg His Pro  Lys Val Tyr 
    1040                 1045                 1050             


Glu Asn  Pro Ile Ala Met Thr  Arg Val Met Gln Lys  Tyr Ala Gln 
    1055                 1060                 1065             


Gln Gln  Ser Lys Glu Phe Asn  Arg Asn His Arg Val  Pro Gly Thr 
    1070                 1075                 1080             


Ser Gly  Phe Ile Val Asn Ser  Phe Met Ser Ser Gly  Phe Asp Lys 
    1085                 1090                 1095             


Glu Thr  Gly Glu Ile Phe Lys  Asp Thr Trp Asn Ala  Glu Thr Glu 
    1100                 1105                 1110             


Ile Glu  Gly Ile Arg Lys Ala  Leu Asn Tyr Arg Gln  Cys Tyr Ile 
    1115                 1120                 1125             


Ser Arg  Met Pro Met Glu Asp  Thr Gly Ala Phe Trp  Asn Ala Thr 
    1130                 1135                 1140             


Val Tyr  Ser Pro Arg Asp Pro  Lys Met Gly Pro Lys  Leu Ala Leu 
    1145                 1150                 1155             


Pro Leu  Lys Lys Gly Leu Asp  Pro Lys Lys Tyr Gly  Gly Phe Ser 
    1160                 1165                 1170             


Ser Gln  Gln Phe Ala Tyr Phe  Phe Val Tyr Glu Ala  Arg Asp Lys 
    1175                 1180                 1185             


Lys Asp  Lys Gln Val Phe Arg  Phe Ala Glu Val Pro  Val Trp Leu 
    1190                 1195                 1200             


Ala Ser  Gln Ile Glu Ser Asp  Lys Ile Ala Leu Glu  Arg Tyr Ala 
    1205                 1210                 1215             


Thr Gly  Leu Ala Glu Ala Glu  Lys Leu Lys Phe Val  Arg Ile Glu 
    1220                 1225                 1230             


Arg Ala  Lys Ile Leu Lys Lys  Gln Leu Ile Glu Ile  Asp Gly Asp 
    1235                 1240                 1245             


Arg Phe  Ile Leu Thr Gly Lys  Lys Glu Met Arg Asn  Gly Arg Glu 
    1250                 1255                 1260             


Leu Ala  Leu Ser Gln Lys Glu  Thr Leu Asn Leu Ile  Val Ala Gly 
    1265                 1270                 1275             


Asp Glu  Asn Ala Ser Leu Asp  Asp Arg Gly Asn Ala  Ser Asp Ala 
    1280                 1285                 1290             


Val Phe  Asp Ala Ile Val Arg  Gln Gly Asp Glu Ile  Gly Leu Arg 
    1295                 1300                 1305             


Leu Tyr  Lys Gln Leu Lys Ile  Ala Glu Ile Ala Glu  Arg Tyr Arg 
    1310                 1315                 1320             


Ser Val  Gly Ala Asp Ala Lys  Val Gly Ala Val Met  Ser Leu Leu 
    1325                 1330                 1335             


Ser Ser  Ile Asn Ala Ser Ser  Asn Met Val Asn Leu  Ser Met Val 
    1340                 1345                 1350             


Gly Gly  Ser Lys Tyr Ala Gly  Asn Leu Gln Pro Asn  Tyr Ser Asn 
    1355                 1360                 1365             


Leu Leu  Ser Asp Pro Glu Thr  Asn Phe Val Ile Ile  Asp Gln Ser 
    1370                 1375                 1380             


Val Thr  Gly Met Phe Glu Arg  Arg Thr Arg Ile Gly  Leu 
    1385                 1390                 1395     


<210>  1165
<211>  1396
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1165

Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asn Arg Asp Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Gly Gly Glu Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Met Gln Val Asp Glu Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Glu Glu Asp Lys Gln 
            100                 105                 110         


Gly Ser Lys Tyr Pro Ile Phe Gly Thr Leu Lys Glu Glu Lys Glu Tyr 
        115                 120                 125             


His Lys Lys Phe Lys Thr Ile Tyr His Leu Arg Glu Glu Leu Ala Asn 
    130                 135                 140                 


Ser Lys Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Tyr Glu Gly Asp Leu Lys Ala 
                165                 170                 175     


Glu Asn Thr Asp Val Gln Ala Leu Phe Lys Asp Phe Val Glu Glu Tyr 
            180                 185                 190         


Asp Lys Thr Ile Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             


Leu Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Ser Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Gln Ala Asn Phe Lys Thr Asn Phe 
                245                 250                 255     


Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Asp Leu Glu Gly Leu Leu Gly Glu Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             


Leu Phe Val Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Val Lys Arg Tyr Lys Glu His Lys Glu Glu Leu Ala Ala Phe Lys 
                325                 330                 335     


Arg Phe Ile Lys Glu Lys Leu Pro Lys Lys Tyr Glu Glu Ile Phe Lys 
            340                 345                 350         


Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Val Gly Ala Asp Lys Lys 
        355                 360                 365             


Leu Arg Lys Arg Ser Gly Lys Leu Ala Thr Glu Glu Glu Phe Tyr Lys 
    370                 375                 380                 


Tyr Val Lys Gly Ile Leu Asn Lys Val Glu Gly Ala Asp Tyr Phe Leu 
385                 390                 395                 400 


Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg Lys Gln Arg Thr Phe Asp 
                405                 410                 415     


Asn Gly Ser Ile Pro His Gln Ile His Leu Gln Glu Met His Ala Ile 
            420                 425                 430         


Leu Arg Arg Gln Gly Glu His Tyr Pro Phe Leu Lys Glu Asn Gln Asp 
        435                 440                 445             


Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
    450                 455                 460                 


Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp Ala Glu Tyr Lys Ala Asp 
465                 470                 475                 480 


Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp Ile Leu Asp Lys Glu Lys 
                485                 490                 495     


Ser Ala Glu Lys Phe Ile Thr Arg Met Thr Leu Asn Asp Leu Tyr Leu 
            500                 505                 510         


Pro Glu Glu Lys Val Leu Pro Lys His Ser Pro Leu Tyr Glu Thr Phe 
        515                 520                 525             


Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Asn Glu Gln Gly 
    530                 535                 540                 


Glu Ala Lys Phe Phe Asp Thr Asn Met Lys Gln Glu Ile Phe Asp His 
545                 550                 555                 560 


Val Phe Lys Glu Asn Arg Lys Val Thr Lys Asp Lys Leu Leu Asn Tyr 
                565                 570                 575     


Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile Val Asn Leu Thr Gly Leu 
            580                 585                 590         


Asp Lys Glu Asn Lys Ala Phe Asn Ala Ser Leu Gly Thr Tyr His Asp 
        595                 600                 605             


Leu Arg Lys Ile Leu Asp Lys Ser Phe Leu Asp Asp Lys Val Asn Glu 
    610                 615                 620                 


Lys Ile Ile Glu Asp Ile Ile Gln Thr Leu Thr Leu Phe Glu Asp Arg 
625                 630                 635                 640 


Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr Ser Asp Ile Phe Thr Thr 
                645                 650                 655     


Gln Gln Leu Lys Glu Leu Glu Arg Arg His Tyr Thr Gly Trp Gly Arg 
            660                 665                 670         


Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys Glu Asn Lys Lys 
        675                 680                 685             


Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Tyr Ala Asn Arg Asn Phe 
    690                 695                 700                 


Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys Glu Glu Ile Ala 
705                 710                 715                 720 


Arg Ala Gln Ile Ile Gly Asp Val Asp Asp Ile Ala Asn Val Val His 
                725                 730                 735     


Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Ser Val 
            740                 745                 750         


Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His Asn Pro Thr Asn 
        755                 760                 765             


Ile Ile Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Asp Lys Gly Arg 
    770                 775                 780                 


Arg Asn Ser Gln Gln Arg Leu Lys Leu Leu Gln Asp Ser Leu Lys Asn 
785                 790                 795                 800 


Leu Asp Asn Pro Val Asn Ile Lys Asn Val Glu Asn Gln Gln Leu Gln 
                805                 810                 815     


Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr 
            820                 825                 830         


Thr Gly Glu Thr Leu Asp Ile Asn Asn Leu Ser Gln Tyr Asp Ile Asp 
        835                 840                 845             


His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn Ser Leu Asp Asn Arg 
    850                 855                 860                 


Val Leu Thr Arg Ser Asp Lys Asn Arg Gly Lys Ser Asp Asp Val Pro 
865                 870                 875                 880 


Ser Ile Glu Val Val His Glu Met Lys Ser Phe Trp Ser Lys Leu Leu 
                885                 890                 895     


Ser Val Lys Leu Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala 
            900                 905                 910         


Glu Arg Gly Gly Leu Thr Glu Asn Asp Lys Ala Gly Phe Ile Lys Arg 
        915                 920                 925             


Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Val Leu 
    930                 935                 940                 


Asp Ala Arg Phe Asn Ala Lys His Asp Glu Asn Lys Lys Val Ile Arg 
945                 950                 955                 960 


Asp Val Lys Ile Ile Thr Leu Lys Ser Asn Leu Val Ser Gln Phe Arg 
                965                 970                 975     


Lys Asp Phe Lys Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His 
            980                 985                 990         


Ala His Asp Ala Tyr Leu Asn Ala  Val Ile Gly Thr Ala  Leu Leu Lys 
        995                 1000                 1005             


Lys Tyr  Pro Lys Leu Ala Ser  Glu Phe Val Tyr Gly  Glu Phe Lys 
    1010                 1015                 1020             


Lys Tyr  Asp Val Arg Lys Phe  Ile Ala Lys Ser Asp  Lys Glu Ile 
    1025                 1030                 1035             


Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr Ser Asn  Leu Met Asn 
    1040                 1045                 1050             


Phe Phe  Lys Lys Glu Val Lys  Phe Ala Asp Gly Thr  Val Val Glu 
    1055                 1060                 1065             


Arg Pro  Asp Ile Glu Thr Ser  Glu Asp Gly Glu Ile  Ala Trp Asn 
    1070                 1075                 1080             


Lys Gln  Thr Asp Phe Lys Ile  Val Arg Lys Val Leu  Ser Tyr Pro 
    1085                 1090                 1095             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Val Gln Thr  His Gly Leu 
    1100                 1105                 1110             


Asp Arg  Gly Lys Pro Arg Gly  Leu Phe Asn Ala Asn  Pro Ser Pro 
    1115                 1120                 1125             


Lys Pro  Lys Pro Asp Ser Ser  Glu Asn Leu Val Gly  Ile Lys Arg 
    1130                 1135                 1140             


Asn Leu  Asp Pro Lys Lys Tyr  Gly Gly Tyr Ala Gly  Ile Ser Asn 
    1145                 1150                 1155             


Ser Tyr  Ala Val Leu Val Lys  Ala Ile Ile Glu Lys  Gly Val Lys 
    1160                 1165                 1170             


Lys Lys  Glu Thr Met Val Leu  Glu Phe Gln Gly Ile  Ser Ile Leu 
    1175                 1180                 1185             


Asp Arg  Ile Thr Phe Glu Lys  Asp Lys Arg Ala Phe  Leu Leu Gly 
    1190                 1195                 1200             


Lys Gly  Tyr Lys Asp Ile Lys  Lys Ile Ile Glu Leu  Pro Lys Tyr 
    1205                 1210                 1215             


Ser Leu  Phe Glu Leu Lys Asp  Gly Ser Arg Arg Met  Leu Ala Ser 
    1220                 1225                 1230             


Ile Leu  Ser Thr Asn Asn Lys  Arg Gly Glu Ile His  Lys Gly Asn 
    1235                 1240                 1245             


Glu Leu  Phe Val Pro Gln Lys  Phe Thr Thr Leu Leu  Tyr His Ala 
    1250                 1255                 1260             


Lys Arg  Ile Asn Asn Pro Ile  Asn Lys Asp His Ile  Glu Tyr Val 
    1265                 1270                 1275             


Lys Lys  His Arg Asp Asp Phe  Lys Glu Leu Leu Asn  Tyr Val Leu 
    1280                 1285                 1290             


Glu Phe  Asn Glu Lys Tyr Val  Gly Ala Thr Lys Asn  Gly Glu Arg 
    1295                 1300                 1305             


Leu Lys  Glu Ala Val Ala Asp  Phe Asp Ser Lys Ser  Asn Glu Glu 
    1310                 1315                 1320             


Ile Cys  Thr Ser Phe Leu Gly  Ala Val Asn Ser Lys  Asn Ala Gly 
    1325                 1330                 1335             


Leu Phe  Glu Leu Thr Ser Leu  Gly Ser Ala Ser Asp  Phe Glu Phe 
    1340                 1345                 1350             


Leu Gly  Val Lys Ile Pro Arg  Tyr Arg Asp Tyr Thr  Pro Ser Ser 
    1355                 1360                 1365             


Leu Leu  Lys Asp Ser Ile Leu  Ile His Gln Ser Ile  Thr Gly Leu 
    1370                 1375                 1380             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Lys Leu Gly Glu  Asp 
    1385                 1390                 1395     


<210>  1166
<211>  1396
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1166

Met Lys Arg Val Asn Glu Asp Tyr Ile Leu Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asn Ser Cys Gly Trp Ala Val Thr Asp Lys Lys Asn Asn Leu Leu Lys 
            20                  25                  30          


Leu Arg Gly Lys Thr Ala Ile Gly Ser His Leu Phe Glu Glu Gly His 
        35                  40                  45              


Thr Ala Ala Asp Arg Arg Gly Phe Arg Thr Thr Arg Arg Arg Leu Lys 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Arg Leu Leu Glu Glu Ile Phe Ala Glu Pro 
65                  70                  75                  80  


Met Ala Lys Val Asp Pro Gly Phe Phe Val Arg Leu His Gln Ser Trp 
                85                  90                  95      


Val Ser Pro Leu Asp Lys Asp Arg Lys Lys Tyr Asn Ala Ile Val Phe 
            100                 105                 110         


Pro Thr Ala Lys Glu Asp Gln Ala Phe Tyr Lys His Tyr Ala Thr Ile 
        115                 120                 125             


Tyr His Leu Arg Asp Glu Leu Met Thr Gln Asp Arg Gln Phe Asp Leu 
    130                 135                 140                 


Arg Glu Ile Phe Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn 
145                 150                 155                 160 


Phe Leu Gln Asp Thr Pro Val Lys Asp Phe Glu Ala Ser Lys Ile Glu 
                165                 170                 175     


Val Gly Pro Ile Leu Ser His Ile Asn Asn Ala Phe Ala Glu Lys Ile 
            180                 185                 190         


Val Glu Asp Gln Asp Pro Ile Glu Leu Asn Val Ala Asn Ala Ala Asp 
        195                 200                 205             


Ile Glu Asp Val Ile Arg Gly Lys Asp Ala Glu Lys Thr Val Tyr Lys 
    210                 215                 220                 


Leu Asp Lys Val Lys Lys Ile Ala Lys Leu Leu Thr Asp Ser Thr Ala 
225                 230                 235                 240 


Lys Glu Glu Lys Asn Val Ala Lys Gln Ile Ala Asn Ala Ile Met Gly 
                245                 250                 255     


Tyr Lys Thr Gln Phe Glu Thr Ile Leu Asp Lys Glu Ile Asp Lys Ser 
            260                 265                 270         


Asp Lys Ala Gln Trp Glu Phe Lys Leu Ser Asp Ala Asp Ala Asp Asp 
        275                 280                 285             


Lys Leu Asp Ala Leu Leu Pro Asp Leu Asp Glu Thr Asp Gln Thr Val 
    290                 295                 300                 


Val Ala Glu Ile Glu Lys Leu Phe Ser Ala Ile Thr Leu Ser Thr Ile 
305                 310                 315                 320 


Val Asp Glu Asn Lys Ser Leu Ser Gln Ser Met Val Glu Lys Tyr Lys 
                325                 330                 335     


Lys His Lys Lys Asp Tyr Lys Lys Leu Lys Lys Tyr Ile Asn Thr Leu 
            340                 345                 350         


Gln Asp Gln Thr Lys Ala Lys Lys Leu Leu Leu Ala Tyr Asp Leu Tyr 
        355                 360                 365             


Val Asn Asn Arg His Gly Arg Leu Leu Glu Ala Lys Lys Thr Phe Glu 
    370                 375                 380                 


Asp Lys Lys Lys Lys Lys Ala Leu Thr Lys Asp Glu Phe Tyr Lys Ile 
385                 390                 395                 400 


Val Lys Asp Asn Leu Asp Asp Ser Asp Leu Ala His Glu Ile Gln Gln 
                405                 410                 415     


Glu Ile Ala Ala Asp Asn Phe Met Pro Lys Gln Arg Thr Asn Ser Asn 
            420                 425                 430         


Gly Val Ile Pro Phe Gln Leu His Gln Ile Glu Leu Asp Lys Ile Ile 
        435                 440                 445             


Ala Asn Gln Gly Lys Tyr Tyr Pro Phe Leu Ala Ala Glu Asn Pro Val 
    450                 455                 460                 


Glu Asp His Arg Lys Gln Ala Pro Tyr Lys Leu Asp Glu Leu Val Arg 
465                 470                 475                 480 


Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Ala Asp Glu Gln 
                485                 490                 495     


Glu Lys Thr Ser Gly Lys Ser Phe Ala Trp Met Val Arg Lys Glu Asp 
            500                 505                 510         


Gly Gln Ile Thr Pro Trp Asn Phe Glu Gln Lys Val Asp Arg Gln Glu 
        515                 520                 525             


Ser Ala Asn Lys Phe Ile Lys Arg Met Thr Ile Lys Asp Thr Tyr Leu 
    530                 535                 540                 


Leu Ser Glu Asp Val Leu Pro Ala Asn Ser Leu Leu Tyr Gln Arg Phe 
545                 550                 555                 560 


Glu Val Leu Asn Glu Leu Asn Asn Ile Arg Ile Asn Gly Ser Arg Ile 
                565                 570                 575     


Ser Val Asp Leu Lys Gln Gln Ile Phe Asn Asp Leu Phe Glu Glu Lys 
            580                 585                 590         


Lys Thr Val Thr Glu Lys Ser Leu Thr Ser Tyr Leu Lys Gln Asn Leu 
        595                 600                 605             


His Leu Pro Thr Val Glu Ile Lys Gly Leu Ala Asp Pro Thr Lys Phe 
    610                 615                 620                 


Asn Ser Ser Leu Ala Ser Tyr Tyr His Leu Lys Ser Leu His Val Phe 
625                 630                 635                 640 


Asp Lys Glu Leu Ala Asp Pro Gln Tyr Gln Lys Asp Phe Glu Lys Ile 
                645                 650                 655     


Ile Glu Tyr Ser Ser Ile Phe Glu Asp Lys Lys Ile Phe Gln Asp Lys 
            660                 665                 670         


Leu His Ala Glu Phe Lys Trp Leu Thr Pro Glu Gln Phe Lys Ala Ile 
        675                 680                 685             


Ser Thr Trp Arg Leu Gln Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu 
    690                 695                 700                 


Val Glu Leu His Asp Thr Asn Gly Gln Asn Ile Met Glu Gln Leu Trp 
705                 710                 715                 720 


Asp Ser Gln Lys Asn Phe Met Gln Ile Val Thr Glu Pro Asp Phe Lys 
                725                 730                 735     


Asp Ala Ile Ala Lys Glu Asn Gln Asn Val Thr Arg Ala Asn Gly Val 
            740                 745                 750         


Glu Glu Ile Leu Ala Asp Ala Tyr Thr Ser Pro Ala Asn Lys Lys Ala 
        755                 760                 765             


Ile Arg Gln Val Val Lys Val Val Ala Asp Val Val Lys Ala Ala Gly 
    770                 775                 780                 


Gly Lys Lys Pro Ala Gln Phe Ala Ile Glu Phe Thr Arg Asp Pro Asp 
785                 790                 795                 800 


Lys Asn Pro Gln Leu Ser His Ile Arg Gly Thr Lys Leu Leu Lys Ala 
                805                 810                 815     


Tyr Gln Glu Thr Ala Gly Glu Leu Val Asp Gln Lys Leu Thr Asp Ser 
            820                 825                 830         


Leu Lys Glu Ala Met Thr Ser Arg Lys Leu Leu Lys Asp Lys Tyr Phe 
        835                 840                 845             


Leu Tyr Phe Met Gln Ala Gly Arg Asp Ala Tyr Thr Gly Gln Lys Ile 
    850                 855                 860                 


Asn Ile Asp Glu Val Ser Thr Asn Tyr Gln Ile Asp His Ile Leu Pro 
865                 870                 875                 880 


Gln Ser Phe Ile Lys Asp Asp Ser Phe Asp Asn Arg Val Leu Thr Ala 
                885                 890                 895     


Thr Pro Leu Asn Ala Glu Lys Ser Asp Asp Val Pro Tyr Lys Arg Phe 
            900                 905                 910         


Ala Asn Asn Tyr Val Ser Asp Met Lys Met Thr Val Gly Glu Met Trp 
        915                 920                 925             


Lys His Trp Gln Lys Ala Gly Ile Ile Asn Lys His Lys Leu Gly Asn 
    930                 935                 940                 


Leu Leu Leu Asp Pro Asp Arg Leu Asn Lys Phe Gln Lys Ser Gly Phe 
945                 950                 955                 960 


Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Ile Ile Lys Leu Val Ser 
                965                 970                 975     


Val Ile Leu Gln Asn Lys Tyr Pro Asp Ala Glu Ile Ile Thr Val Lys 
            980                 985                 990         


Ala Gly Asp Asn Ser Ala Leu Arg  Gln Arg Leu Asn Leu  Tyr Lys Ser 
        995                 1000                 1005             


Arg Asp  Val Asn Asp Tyr His  His Ala Ile Asp Ala  Tyr Leu Ser 
    1010                 1015                 1020             


Ile Ile  Cys Gly Asn Phe Leu  Tyr Gln Val Tyr Pro  Lys Tyr Arg 
    1025                 1030                 1035             


Pro Tyr  Phe Val Tyr Gly Lys  Tyr Lys Lys Phe Ser  Gln Asp Pro 
    1040                 1045                 1050             


Asp Leu  Gln Lys Glu Val Ile  Lys His Phe Lys Gly  Phe Thr Phe 
    1055                 1060                 1065             


Met Trp  Pro Leu Leu Gln Lys  Asp Asn Ser Glu Arg  Lys Ala Pro 
    1070                 1075                 1080             


Glu Lys  Ile Lys Glu Asn Asn  Ser Asp Arg Ile Val  Phe Tyr Lys 
    1085                 1090                 1095             


His Pro  Asp Ile Phe Asp Lys  Leu Arg Lys Ala Tyr  Asn Tyr Lys 
    1100                 1105                 1110             


Tyr Met  Leu Val Ser Arg Glu  Thr Thr Thr Glu Asn  Ser Gly Leu 
    1115                 1120                 1125             


Phe Asp  Val Thr Ile Tyr Pro  Arg Gly Glu Arg Asp  Leu Ala Lys 
    1130                 1135                 1140             


Thr Arg  Lys Leu Ile Pro Lys  Ser Asn Gly Leu Asp  Pro Lys Ile 
    1145                 1150                 1155             


Tyr Gly  Gly Tyr Ser Gly Asn  Thr Asp Ala Tyr Met  Val Ile Val 
    1160                 1165                 1170             


Lys Ile  Asp Lys Gly Lys Glu  Ser Ile Tyr Lys Val  Ile Gly Val 
    1175                 1180                 1185             


Pro Met  Arg Ala Leu Ala Ser  Leu Asn Arg Ala Lys  Lys Gln Gly 
    1190                 1195                 1200             


Asn Tyr  Lys Glu Glu Leu His  Gln Val Leu Glu Pro  Gln Ile Met 
    1205                 1210                 1215             


Phe Asp  Lys Asn Gly Lys Pro  Lys Arg Ser Val Lys  Gly Phe Arg 
    1220                 1225                 1230             


Ile Ile  Lys Asp His Val Pro  Phe Lys Gln Val Val  Leu Asp Gly 
    1235                 1240                 1245             


Asp Lys  Lys Phe Met Leu Asn  Ser Ser Thr Tyr Glu  Ile Asn Ala 
    1250                 1255                 1260             


Lys Gln  Leu Thr Leu Thr Pro  Glu Thr Met Arg Ile  Val Thr Asp 
    1265                 1270                 1275             


Asn Leu  Lys Lys Gly Glu Asp  Gln Asp Gln Leu Leu  Val Lys Ala 
    1280                 1285                 1290             


Tyr Asp  Glu Ile Leu Gln Lys  Val Asp Gln Tyr Leu  Pro Leu Phe 
    1295                 1300                 1305             


Asp Val  Asn Lys Phe Arg Asn  Ser Leu His Leu Gly  Arg Ala Lys 
    1310                 1315                 1320             


Phe Leu  Asp Leu Ala Val Asn  Asp Lys Lys Ile Thr  Leu Thr Asn 
    1325                 1330                 1335             


Ile Leu  Asn Gly Leu His Asp  Asn Leu Val Thr Pro  Asp Leu Lys 
    1340                 1345                 1350             


Asn Ile  Gly Ile Lys Thr Pro  Leu Gly Lys Leu Gln  Val Pro Ser 
    1355                 1360                 1365             


Gly Ile  Val Leu Ser Ser Glu  Ala Ile Leu Ile Phe  Gln Ser Pro 
    1370                 1375                 1380             


Thr Gly  Leu Phe Glu Lys Arg  Val Arg Ile Ala Asp  Leu 
    1385                 1390                 1395     


<210>  1167
<211>  1397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1167

Met Lys Asp Tyr Asn Ile Gly Leu Asp Ile Gly Thr Ser Ser Ile Gly 
1               5                   10                  15      


Phe Ala Ala Ile Asp Asp Asp Asn Asn Leu Ile Arg Val Lys Gly Lys 
            20                  25                  30          


Asn Val Ile Gly Ser Arg Leu Phe Ala Glu Gly Lys Thr Ala Ala Glu 
        35                  40                  45              


Arg Arg Gly Phe Arg Thr Ala Arg Arg Arg Tyr Asn Arg Arg Lys Trp 
    50                  55                  60                  


Arg Leu Lys Leu Leu Asp Glu Ile Phe Tyr Glu Glu Ile Ile His Ile 
65                  70                  75                  80  


Asp Pro Glu Phe Phe Asn Arg Leu Lys Gln Ser Ser Ile Ser Pro Lys 
                85                  90                  95      


Asp Glu Ser Lys Lys Tyr Phe Gly Ser Leu Leu Phe Pro Asp Lys Thr 
            100                 105                 110         


Asp Ser Ala Tyr Tyr His Asp Gly Asn Gln Thr Ile Tyr His Leu Arg 
        115                 120                 125             


Asn Arg Leu Val His Gln Lys Glu Lys Ala Asp Ile Arg Glu Ile Tyr 
    130                 135                 140                 


Leu Ala Leu Arg His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asp Ser 
145                 150                 155                 160 


Thr Pro Val Ser Ser Phe Glu Ala Asp Asn Leu Lys Leu Asp Glu Leu 
                165                 170                 175     


Val Pro Val Ile Asn Asp Met Tyr Glu Asn Ile Asp Ile Asp Phe Ser 
            180                 185                 190         


Ile Asn Val Asp Asn Ile Ser Glu Ile Glu Lys Val Leu Leu Asp Asn 
        195                 200                 205             


His Leu Lys Ser Ala Asp Lys Gln Asp Arg Leu Lys Glu Leu Leu Tyr 
    210                 215                 220                 


Asp Asn Pro Glu Tyr Pro Asp Asp Lys Val Lys Gln Lys Glu Ile Ser 
225                 230                 235                 240 


Lys Phe Arg Lys Asp Leu Asp Lys Glu Ile Ile Lys Val Ile Val Gly 
                245                 250                 255     


Tyr Lys Phe Asn Ala Tyr Lys Ile Val Asn Tyr Ile Pro Asp Asp Gly 
            260                 265                 270         


Ser Lys Phe Ser Phe Ser Phe Ser Asp Glu Ser Ser Asp Asp Lys Leu 
        275                 280                 285             


Asp Asp Leu Ser Glu Val Leu Asp Asp Ser Gln Met Glu Ile Leu Asn 
    290                 295                 300                 


Thr Leu Lys Val Ile Tyr Ser Arg Val Arg Leu Asn Gln Ile Ile Pro 
305                 310                 315                 320 


Asn Gly Met Gly Leu Ser Glu Ser Met Val Ala Lys Tyr Asn Arg His 
                325                 330                 335     


His Glu Gln Leu Glu Asp Leu Lys Glu Leu Leu Asp Val Leu Pro Asn 
            340                 345                 350         


Asp Glu Lys Arg Ser Ile Glu Tyr Ala Tyr Thr Ala Tyr Ile Gly Asn 
        355                 360                 365             


Val Asp Lys Gln Lys Tyr Asn Asn Asn Leu Val Ala Ser Leu Lys Asp 
    370                 375                 380                 


Leu Pro Ile Lys Gly Asp Ile Asn Lys Lys Ile Gln Ala Val Phe Glu 
385                 390                 395                 400 


Tyr Ala Lys Gly His Ile Ser Ser Gln Asp Leu Tyr Asp Val Ile Lys 
                405                 410                 415     


Thr Ile Ile Ile Asp Lys Tyr Leu Glu Asp Glu Ser Phe Glu Tyr His 
            420                 425                 430         


Asp Leu Val Glu Arg Ile Ser Asn Asp Ile Asp Asn Lys Asp Tyr Leu 
        435                 440                 445             


Leu Lys Gln Arg Ser Ser Gln Asn Gly Asn Ile Pro His Gln Val His 
    450                 455                 460                 


Gln Met Glu Met Asp Lys Ile Ile Ala Asn Gln Ser Gln Tyr Tyr Pro 
465                 470                 475                 480 


Phe Leu Gly Val Leu Asn Pro Asn Glu Ser Arg Arg His Ile Ala Lys 
                485                 490                 495     


Tyr Lys Ile Ser Glu Leu Val Ser Phe Arg Val Pro Tyr Tyr Val Gly 
            500                 505                 510         


Pro Leu Ile Thr Pro Glu Glu Gln Glu Lys Thr Ser Gly Lys His Phe 
        515                 520                 525             


Ala Trp Met Lys Arg Lys Glu Ala Gly Lys Ile Thr Pro Trp Asn Phe 
    530                 535                 540                 


Glu Glu Lys Val Asp Lys Glu Ala Thr Ala Ala Lys Phe Ile Lys Arg 
545                 550                 555                 560 


Leu Thr Val Lys Asp Thr Tyr Leu Leu Asn Glu Asp Val Leu Pro Asp 
                565                 570                 575     


Asn Ser Leu Leu Tyr Gln Gln Phe Lys Val Leu Asn Glu Leu Asn Ile 
            580                 585                 590         


Val Lys Val Asn Asn Lys Arg Leu Thr Leu Ser Glu Lys Gln Gly Val 
        595                 600                 605             


Tyr Asn Asp Leu Phe Lys Asn Thr Lys Ser Ile Thr Val Lys Arg Leu 
    610                 615                 620                 


Lys Asn Tyr Leu Ile Ser Asn Tyr Lys His Leu Glu Asn Ile Glu Ile 
625                 630                 635                 640 


Thr Gly Leu Ser Asp Gly Asp Lys Phe Asn Asn Ser Leu Gly Thr Tyr 
                645                 650                 655     


Asn Asp Phe Lys Lys Ile Phe Gly Ala Arg Ile Asp Asp Val Asn Tyr 
            660                 665                 670         


Ser Ala Asp Phe Glu Asn Ile Ile Glu Trp Ser Thr Val Phe Glu Asp 
        675                 680                 685             


Arg Ser Ile Leu Lys Glu Lys Leu Glu Lys Asp Ile Asp Trp Ile Thr 
    690                 695                 700                 


Pro Glu Glu Ile Lys Ala Val Val Ala Lys Arg Tyr Ser Gly Trp Gly 
705                 710                 715                 720 


Arg Leu Ser Ala Lys Leu Leu Thr Glu Leu Leu Asp Asp Asp Gly Gln 
                725                 730                 735     


Arg Ile Ile Asp Ile Leu Trp Asn Asp Ser Ile Thr Phe Met Glu Ala 
            740                 745                 750         


Val Ser Lys Pro Glu Ile Lys Ala Gln Ile Gln Asn Ile Asn Arg Asp 
        755                 760                 765             


Phe Ile Lys Ala Thr Asp Ile Glu Thr Val Leu Asp Glu Ala Tyr Thr 
    770                 775                 780                 


Ser Pro Gln Asn Lys Lys Ala Ile Arg Glu Val Tyr Lys Leu Val Lys 
785                 790                 795                 800 


Asp Ile Gln Lys Ala Met Gly Gly Lys Ala Pro Ser Ser Ile Ser Ile 
                805                 810                 815     


Glu Phe Thr Arg Ser Pro Glu Asp Asn Gly Ser Val Thr Lys Thr Arg 
            820                 825                 830         


Tyr Asn Asn Met Gln Asn Lys Tyr Lys Glu Leu Gly Lys Glu Leu Ser 
        835                 840                 845             


Asp Asp Leu Ser Ala Gln Leu Lys Glu Tyr Lys Gly Arg Met Asp Gln 
    850                 855                 860                 


Asp Lys Phe Tyr Leu Tyr Phe Met Gln Met Gly Lys Asp Leu Tyr Asp 
865                 870                 875                 880 


Gly Ser Thr Ile Asn Leu Asp Asp Leu Asn Ser Tyr Gln Ile Asp His 
                885                 890                 895     


Ile Val Pro Gln Ser Phe Tyr Lys Asp Asp Ser Ile Asp Asn Arg Val 
            900                 905                 910         


Leu Thr His Tyr Thr Asn Asn Gln Lys Lys Gly Asn His Thr Pro Leu 
        915                 920                 925             


Asn Gly Leu Asn Ile Asp Ala Gln Thr Gln Asn Leu Trp Lys His Leu 
    930                 935                 940                 


Asn Lys Leu Gly Leu Ile Ser Lys Lys Lys Leu Gln Asn Leu Cys Phe 
945                 950                 955                 960 


Lys Asp Ile Asn Gln Leu Ser Lys Tyr Thr Lys His Gly Phe Val Arg 
                965                 970                 975     


Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Val Ala Asn Ile 
            980                 985                 990         


Leu Ser Asp Glu Phe Ala Asn Asp  Gly Thr Lys Ile Ile  Glu Val Lys 
        995                 1000                 1005             


Ala Lys  Met Asn Ser Leu Met  Arg Asp Thr Phe Asn  Leu Tyr Lys 
    1010                 1015                 1020             


Val Arg  Gln Leu Asn Asp Tyr  His His Ala Val Asp  Ala Tyr Leu 
    1025                 1030                 1035             


Thr Thr  Phe Val Gly Lys Tyr  Leu Tyr Lys Arg Tyr  Pro Lys Leu 
    1040                 1045                 1050             


Arg Ser  Met Phe Val Tyr Gly  Asp Phe Lys Lys Phe  Ser Ala Glu 
    1055                 1060                 1065             


Asp Asp  Leu Lys Asp Ile Lys  Thr Phe Asn Phe Leu  His Asp Ile 
    1070                 1075                 1080             


Thr Asn  Pro Lys Glu Ser Asn  Lys Glu Lys Ile Tyr  Asp Glu Lys 
    1085                 1090                 1095             


Ser Gly  Glu Leu Ile Leu Asp  Arg Lys His Ala Ile  Glu Thr Ile 
    1100                 1105                 1110             


Lys Lys  Ile Tyr Gln Tyr Lys  Phe Met Leu Ile Val  Arg Glu Val 
    1115                 1120                 1125             


Ser Thr  Lys Ser Gly Gln Leu  Phe Lys Gln Thr Ile  Tyr Ser Ala 
    1130                 1135                 1140             


Lys Lys  Thr Asn Gly Leu Lys  Ser Ala Ile Pro Val  Lys Ser Asn 
    1145                 1150                 1155             


Leu Glu  Pro Ser Ile Tyr Gly  Tyr Arg Ser Gly Asn  Val Asp His 
    1160                 1165                 1170             


His Met  Val Ile Val Lys Ile  Lys Lys Gly Thr Lys  Tyr Ser Phe 
    1175                 1180                 1185             


Lys Val  Ile Gly Ile Ser Ala  Arg Ala Ile Gln Glu  Leu Glu Ile 
    1190                 1195                 1200             


Ala Lys  Lys Ile Gly Asn Tyr  Arg Asp Lys Leu Tyr  Glu Val Val 
    1205                 1210                 1215             


Ser Asp  Arg Leu Ser Gln Ser  Met Lys Ser Val Glu  Ser Phe Arg 
    1220                 1225                 1230             


Ile Ile  Leu Asp Asn Ile Met  Tyr Gly Gln Leu Ile  Val Asp Gly 
    1235                 1240                 1245             


Asn Asp  Lys Phe Thr Leu Gly  Ser Ala Asp Tyr Arg  Lys Asn Ser 
    1250                 1255                 1260             


Arg Gln  Leu Val Leu Cys Thr  Asn Ser Leu Lys Ile  Leu Lys Asp 
    1265                 1270                 1275             


Glu Asp  Tyr Met Asn Arg Leu  Ser Asp Ile Gln Arg  Ser Lys Leu 
    1280                 1285                 1290             


Met Val  Gln Val Tyr Asp Glu  Ile Ile Lys Ile Met  Lys Asn Lys 
    1295                 1300                 1305             


Tyr Ser  Leu Phe Ile Ser Asn  Thr Glu Lys Leu Leu  Glu Lys Arg 
    1310                 1315                 1320             


Asp Ile  Phe Cys Gly Leu Ser  Phe Asp Glu Gln Lys  Ile Val Ile 
    1325                 1330                 1335             


Trp Lys  Ile Ile Ile Asp Leu  His Asn Gly Pro Phe  Thr Lys Ile 
    1340                 1345                 1350             


Gln Glu  Leu Gly Ile Asn Asn  Lys Met Gly Thr Phe  Gln Thr Lys 
    1355                 1360                 1365             


His Gly  Ile Ile Leu Thr Glu  Asn Ala Ile Val Ile  Tyr Gln Ser 
    1370                 1375                 1380             


Pro Ser  Gly Leu Phe Glu Arg  Lys Val Arg Leu Lys  Asp Leu 
    1385                 1390                 1395         


<210>  1168
<211>  1397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1168

Met Lys Lys Val Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Thr Lys Glu Glu Lys Lys Glu Gly Lys Ser 
            20                  25                  30          


Ser Glu Ile Ile Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp 
        35                  40                  45              


Glu Lys Thr Asn Phe Glu Lys Gly Arg Pro Leu Ser Thr Asn Ala Asp 
    50                  55                  60                  


Arg Thr Leu Lys Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu 
65                  70                  75                  80  


Arg Arg Arg Asn Leu Ile Glu Ile Leu Ile Lys Asn Gly Phe Ile Thr 
                85                  90                  95      


Lys Asp Thr Pro Leu Thr Glu Thr Gly Lys Asp Thr Thr His Gln Thr 
            100                 105                 110         


Leu Lys Leu Arg Ala Lys Ser Ala Lys Glu Lys Val Asp Leu Lys Asp 
        115                 120                 125             


Phe Ala Lys Ile Leu Leu Thr Ile Asn Lys Lys Arg Gly Tyr Lys Ser 
    130                 135                 140                 


Ser Arg Lys Val Asn Ser Asp Glu Asp Gly Leu Ala Ile Asp Gly Met 
145                 150                 155                 160 


Ala Val Ala Lys His Leu Tyr Glu Gln Asn Leu Thr Pro Gly Gln Tyr 
                165                 170                 175     


Val Leu Asn Leu Leu Gln Lys Asn Lys Lys Tyr Val Pro Asp Phe Tyr 
            180                 185                 190         


Arg Ser Asp Leu Gln Glu Glu Phe Lys Asn Ile Trp Asn Asn Gln Lys 
        195                 200                 205             


Thr Tyr Tyr Phe Asp Ile Leu Thr Asp Thr Leu Phe Glu Glu Leu Lys 
    210                 215                 220                 


Asp Lys Asn Lys Ser Gln Thr Trp Ala Ile Cys Lys Lys Pro Phe Asp 
225                 230                 235                 240 


Ile Ala Gly Ile Lys Gln Ile Gly Lys Ala Ser Asp Lys Arg Leu Glu 
                245                 250                 255     


Arg Tyr Lys Trp Arg Thr Glu Gly Leu Thr Gln Lys Leu Asn Leu Glu 
            260                 265                 270         


Tyr Leu Ala Ile Ala Leu Gln Glu Ile Asn Asn Asp Ile Asn Lys Ser 
        275                 280                 285             


Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Phe Phe 
    290                 295                 300                 


Asn Lys Glu Thr Val Gly Glu Asn Leu Tyr Arg Gln Leu Val Lys Thr 
305                 310                 315                 320 


Pro His Thr Ser Leu Arg Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu 
                325                 330                 335     


Asp Glu Phe Glu Thr Ile Trp Glu Thr Gln Ala Lys Phe Tyr Pro Gln 
            340                 345                 350         


Leu Thr His Thr Leu Lys Glu Glu Ile Arg Asp Val Ile Ile Phe Tyr 
        355                 360                 365             


Gln Arg Lys Leu Lys Ser Gln Lys Ser Leu Ile Ser Phe Cys Gln Phe 
    370                 375                 380                 


Glu Ser Trp Glu Gln Thr Tyr Ile Glu Lys Glu Thr Gly Lys Ser Lys 
385                 390                 395                 400 


Thr Arg Thr Val Gly Arg Lys Val Ile Pro Lys Ser Ser Pro Leu Phe 
                405                 410                 415     


Gln Glu Phe Lys Ile Trp Gln Asn Leu Asn Ser Leu Glu Phe Arg Asn 
            420                 425                 430         


Ser Asp Glu Gly Glu Ile Ile Glu Phe Ile Lys Leu Asp Glu Asp Ile 
        435                 440                 445             


Arg Gln Thr Val Phe Glu Glu Leu Asn Leu Arg Gly Asn Leu Lys Pro 
    450                 455                 460                 


Asn Asp Val Leu Lys Ile Leu Gly Asn Tyr Ile Ser Leu Gly Lys Val 
465                 470                 475                 480 


Ser Gln Trp Lys Cys Asn Phe Glu Glu Ile Glu Gly Asn Arg Thr Asn 
                485                 490                 495     


Ala Ala Leu Tyr Asn Val Tyr Gln Ile Ile Ala Glu Asn Glu Gly Tyr 
            500                 505                 510         


Gly Phe Asp Trp Ala Lys Lys Thr Ala Ser Glu Ile Asn Lys Glu Leu 
        515                 520                 525             


Lys Asn Ile Phe Pro Gln Leu Gly Ile Asp Ala Ser Ile Leu Asp Phe 
    530                 535                 540                 


Asp Phe Thr Gln Ala Asn Phe Asp Lys Gln Thr Ser Tyr Gln Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Ala Glu Glu Asp Phe Lys Ile Ser Glu Glu Asp 
                565                 570                 575     


Lys Val Leu Tyr Gly Asn Ser Ser Val Ser Leu Lys Lys Lys Leu His 
            580                 585                 590         


Gln Lys Tyr Gly Phe Lys Pro Glu Tyr Thr Lys Leu Leu Thr Asn Ile 
        595                 600                 605             


Ser Leu Gln Pro Asp Tyr Gly Asn Leu Ser Ala Lys Ala Ile Cys Asn 
    610                 615                 620                 


Ile Leu Pro Tyr Leu Lys Glu Asn Asn Val Tyr Ser Glu Ala Cys Asp 
625                 630                 635                 640 


Leu Thr Gly Tyr Asn His Ser Asn Phe Leu Thr Ala Glu Glu Gln Ala 
                645                 650                 655     


Lys Arg Pro Leu Lys Pro Ile Leu Glu Leu Leu Lys Lys Asn Ser Leu 
            660                 665                 670         


Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Met Val Asn Val Val 
        675                 680                 685             


Asn Gln Val Ile Glu Thr Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu 
    690                 695                 700                 


Leu Thr Arg Glu Leu Lys Lys Ser Ala Lys Glu Arg Ala Asn Met Thr 
705                 710                 715                 720 


Lys Ser Ile Ala Asp Ala Thr Arg Arg Asn Asp Asp Tyr Arg Lys Thr 
                725                 730                 735     


Ile Ile Lys Glu Phe Gly Ile Pro Asn Pro Thr Lys Ser Asp Val Val 
            740                 745                 750         


Arg Tyr Arg Leu Trp Gln Glu Leu Ala Ser Arg Gly Tyr Lys Asp Ile 
        755                 760                 765             


Phe Thr Asp Thr Glu Ile Lys Pro Gln Glu Leu Phe Ser Asn Lys Ile 
    770                 775                 780                 


Asp Ile Glu His Ile Ile Pro Lys Ala Leu Leu Phe Asp Asp Ser Phe 
785                 790                 795                 800 


Ser Asn Lys Thr Leu Ala Phe Lys Lys Asp Asn Leu Lys Lys Ala Asn 
                805                 810                 815     


Arg Thr Ala Tyr Asp Phe Ile Ala Asn Asp Tyr Ala Pro Asn Leu Glu 
            820                 825                 830         


Asn Tyr Ile Glu Arg Val Glu Ser Leu His Lys Asp Arg Lys Ile Thr 
        835                 840                 845             


Arg Ala Lys Arg Asn Lys Leu Leu Met Leu Gln Ser Lys Leu Pro Asp 
    850                 855                 860                 


Gly Phe Ile Glu Arg Asp Leu Arg Asn Ser Gln Tyr Ile Thr Lys Glu 
865                 870                 875                 880 


Ala Lys Lys Met Leu Tyr Glu Val Cys Arg Thr Val Val Ser Thr Ser 
                885                 890                 895     


Gly Ser Ile Thr Asp Lys Leu Arg Glu Asp Trp Asp Leu Ile Asn Val 
            900                 905                 910         


Met Lys Glu Leu Asn Phe Pro Lys Tyr Lys Ala Leu Gly Leu Thr Glu 
        915                 920                 925             


Lys Ile Glu Arg Tyr Asp Ile Gly Ala Glu Lys Met Lys Glu Val Glu 
    930                 935                 940                 


Ile Ile Glu Asp Trp Asn Lys Arg Asn Asp His Arg His His Ala Ile 
945                 950                 955                 960 


Asp Ala Leu Thr Val Ala Phe Ser Thr His Ser His Ile Gln Tyr Leu 
                965                 970                 975     


Asn His Leu Asn Ala Arg Arg Asp Glu Gly His Lys Lys His Ala Ala 
            980                 985                 990         


Ile Ile Ala Ile Glu Ser Thr Ile  Thr Glu Lys Glu Gly  Asn Lys Lys 
        995                 1000                 1005             


Arg Arg  Phe Ile Ala Pro Leu  Pro Asn Phe Arg Leu  Gln Ala Lys 
    1010                 1015                 1020             


Glu His  Ile Glu Ser Ile Leu  Val Ser Phe Lys Asn  Lys Asn Lys 
    1025                 1030                 1035             


Val Val  Thr Asn Asn Ile Asn  Lys Thr Lys Arg Ile  Lys Lys Asp 
    1040                 1045                 1050             


Lys Phe  Asn Thr Lys Val Gln  Leu Thr Pro Arg Gly  Gln Leu His 
    1055                 1060                 1065             


Lys Glu  Thr Ile Tyr Gly Lys  Ile Lys Gln Pro Leu  Glu Lys His 
    1070                 1075                 1080             


Ile Lys  Leu Asn Lys Ser Phe  Thr Leu Glu Gln Ala  Lys Leu Ile 
    1085                 1090                 1095             


Ile Asp  Lys Arg Gln Arg Glu  Lys Val Leu Asn His  Leu Glu Lys 
    1100                 1105                 1110             


Tyr Ala  His Asn Ser Asp Ile  Ala Phe Asn Ser Lys  Thr Leu Lys 
    1115                 1120                 1125             


Lys Asn  Pro Leu Leu Ile Ser  Asp Glu Pro Leu Lys  Glu Val Leu 
    1130                 1135                 1140             


Cys Phe  Glu Glu Val Phe Thr  Ile Arg Lys Asp Ile  Ser Tyr Asp 
    1145                 1150                 1155             


Asn Phe  Lys Asn Glu Lys Gln  Leu Asp Lys Val Ile  Asp Glu Lys 
    1160                 1165                 1170             


Val Lys  Gln Ala Leu Lys Glu  Arg Ile Asn Gln Tyr  Asn Gly Asp 
    1175                 1180                 1185             


Phe Arg  Glu Ala Phe Ser Asp  Ile Glu Arg Asn Pro  Ile Trp Leu 
    1190                 1195                 1200             


Asn Lys  Ala Lys Gly Ile Cys  Val Lys Arg Ala Thr  Ile Lys Gly 
    1205                 1210                 1215             


Val Ser  Asn Ala Glu Pro Leu  His Thr Ala Lys Asn  His Leu Gly 
    1220                 1225                 1230             


Glu Glu  Val Leu Thr Glu Asn  Lys Glu Gln Ile Pro  Val Asp Tyr 
    1235                 1240                 1245             


Val Ser  Thr Gly Asn Asn His  His Val Ala Ile Tyr  Glu Asp Glu 
    1250                 1255                 1260             


Asp Gly  Asn Leu Gln Glu Lys  Val Val Ser Phe Tyr  Glu Ala Val 
    1265                 1270                 1275             


Ile Arg  Val Asn Glu Gly Leu  Pro Ile Ile Asp Lys  Glu Tyr Asn 
    1280                 1285                 1290             


Lys Ser  Leu Gly Trp Thr Phe  Lys Phe Thr Met Lys  Gln Asn Glu 
    1295                 1300                 1305             


Met Phe  Val Phe Pro Ser Glu  Asp Phe Asn Pro Thr  Glu Ile Asp 
    1310                 1315                 1320             


Leu Leu  Asp Gln Lys Asn Ala  Ser Leu Ile Ser Pro  Asn Leu Phe 
    1325                 1330                 1335             


Arg Val  Gln Lys Phe Thr Ile  Lys Asp Tyr Phe Phe  Arg His His 
    1340                 1345                 1350             


Leu Glu  Thr Thr Val Glu Asn  Asn Asn Thr Leu Lys  Asn Thr Thr 
    1355                 1360                 1365             


Trp Arg  Arg Glu Gly Leu Ser  Gly Ile Tyr Gly Ile  Ile Lys Ile 
    1370                 1375                 1380             


Arg Leu  Asn His Leu Gly Glu  Ile Val Gln Val Gly  Glu Tyr 
    1385                 1390                 1395         


<210>  1169
<211>  1397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1169

Met Asn Glu Asn Lys Glu Trp Phe Leu Gly Leu Asp Ile Gly Thr Ala 
1               5                   10                  15      


Ser Val Gly Trp Ala Ala Thr Asp Thr Asn Tyr Asn Leu Leu Lys Leu 
            20                  25                  30          


Lys Gly Lys Lys Ser Trp Gly Val Arg Leu Phe Asp Glu Ala Gln Thr 
        35                  40                  45              


Ala Lys Glu Arg Arg Asp Cys Arg Ser Lys Arg Arg Arg Leu Ala Arg 
    50                  55                  60                  


Arg Lys Trp Arg Leu Gly Leu Leu Glu Glu Leu Phe Ala Glu Glu Ile 
65                  70                  75                  80  


Asn Lys Ile Asp Pro Asn Phe Phe Met Arg Leu Arg Glu Ser Lys Tyr 
                85                  90                  95      


Lys Val Ser Asp Lys Thr Gly Asn Val Pro Ser Ile Leu Phe Asn Glu 
            100                 105                 110         


Lys Asn Tyr Thr Asp Lys Glu Tyr Tyr Arg Asn Tyr Lys Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Ala Asp Leu Ile His Lys Asn Asn Pro Asp Ile Arg Glu 
    130                 135                 140                 


Leu Phe Leu Ala Ile His His Ile Leu Lys Tyr Arg Gly His Phe Leu 
145                 150                 155                 160 


Phe Ala Gly Gln Gln Phe Lys Gly Ser Glu Ile Lys Asp Asn Ile Arg 
                165                 170                 175     


Glu Leu Leu Gln Tyr Asn Ser Leu Asn Ile Lys Ile Asp Ile Thr Asp 
            180                 185                 190         


Glu Leu Ile Asn Asn Ile Ala Glu Ile Leu Leu Lys Lys Lys Asn Ser 
        195                 200                 205             


Thr Asp Lys Ile Ile Glu Phe Lys Lys Leu Phe Ala Asp Glu Lys Gln 
    210                 215                 220                 


Leu Ile Glu Ala Leu Arg Leu Ala Leu Gly Asn Lys Thr Gln Leu Ala 
225                 230                 235                 240 


Lys Leu Phe Asp Ile Glu Ala Tyr Lys Glu Leu Asp Ile Ser Ile Lys 
                245                 250                 255     


Thr Ile Ser Phe Lys Glu Lys Ile Tyr Glu Asp Val Arg Ser Asn Tyr 
            260                 265                 270         


Glu Glu Ile Leu Gly Glu Tyr Ile Ile Val Leu Asp Thr Ala Lys Leu 
        275                 280                 285             


Ile Tyr Asp Ala Ile Ile Leu Ser Lys Ile Lys Ile Lys Asn Met Thr 
    290                 295                 300                 


Ile Ser Glu Ser Lys Val Val Ser Tyr Glu Lys His Gly Lys Asp Leu 
305                 310                 315                 320 


Lys Gln Leu Lys His Leu Ile Lys Glu Asp Cys Lys Leu Ser Lys Lys 
                325                 330                 335     


Glu Lys Arg Gln Leu Phe Glu Asn Ile Phe Val Lys Glu Asp Glu Lys 
            340                 345                 350         


Gly Ala Asn Tyr Val Asn Tyr Ile Gly Lys Asn Asn Ala Gly Ser Ser 
        355                 360                 365             


Lys Ser Ser Cys Ser Tyr Glu Asp Phe Lys Lys Phe Leu Thr Lys Glu 
    370                 375                 380                 


Leu Lys Cys Cys Glu Glu Ser Lys Leu Lys Gln Asp Ile Leu Ala Glu 
385                 390                 395                 400 


Leu Glu Leu Glu Thr Phe Leu Pro Arg Gln Arg Thr Lys Asp Asn Ser 
                405                 410                 415     


Val Ile Leu Tyr Gln Ile His Lys Glu Glu Leu Glu Lys Ile Leu Asn 
            420                 425                 430         


Asn Ala Val Gln Tyr His Pro Phe Leu Asn Asn Thr Asp Glu Tyr Gly 
        435                 440                 445             


Ile Ser Ile Lys Glu Lys Ile Ile Gln Leu Leu Glu Phe Arg Ile Pro 
    450                 455                 460                 


Tyr Tyr Val Gly Pro Leu Asn Thr Ser His Lys Val Gly Asp Gly Gly 
465                 470                 475                 480 


Phe Ser Trp Ser Val Arg Asn Lys Gly Tyr Glu His Ile Ala Val Thr 
                485                 490                 495     


Pro Trp Asn Phe Asn Lys Val Ile Asn Lys Glu Glu Ser Ala Lys Asn 
            500                 505                 510         


Phe Ile Glu Asn Leu Thr Asn Gln Cys Thr Tyr Leu Leu Tyr Glu Asp 
        515                 520                 525             


Val Leu Pro Lys His Ser Leu Leu Tyr Ser Glu Phe Ala Leu Leu Asn 
    530                 535                 540                 


Glu Leu Asn Ser Ile Asn Tyr Gly Gly Lys Arg Leu Thr Val Glu His 
545                 550                 555                 560 


Arg Asn Leu Ile Ile Glu Arg Phe Phe Lys Gln Ser Ala Pro Lys Ala 
                565                 570                 575     


Thr Gly Lys Glu Leu Lys Lys Phe Leu Met Ser Glu Gly Val Ile Asp 
            580                 585                 590         


Gly Lys Asp Glu Leu Ser Gly Phe Asp Val Ser Ile Lys Asn Asp Leu 
        595                 600                 605             


Lys Ser Tyr Arg Asp Phe Lys Lys Ile Leu Gly Asp Lys Phe Asp Ser 
    610                 615                 620                 


Glu Lys Val Glu Lys Met Ile Arg Trp Ile Thr Leu Phe Gly Glu Ser 
625                 630                 635                 640 


Lys Gln Leu Leu Val Asp Lys Ile Thr Lys Glu Tyr Gly Asp Ile Tyr 
                645                 650                 655     


Ser Leu Glu Glu Ile Lys Lys Ile Ser Gly Leu Thr Tyr Lys Asp Trp 
            660                 665                 670         


Gly Arg Leu Ser Glu Lys Leu Leu Thr Glu Ile Val Ser Ser Gln Leu 
        675                 680                 685             


Tyr Asp Glu Ser Thr Gly Glu Cys Phe Asn Ile Ile Asn Ala Met Arg 
    690                 695                 700                 


Asn Asn Thr Leu Leu Phe Met Glu Leu Leu Gly Thr Asn Tyr Asp Tyr 
705                 710                 715                 720 


Phe Glu Lys Ile Asn Ile Tyr Asn Arg Arg Leu Arg Lys Glu Ile Thr 
                725                 730                 735     


Glu Ile Thr Pro His Ile Leu Asp Asp Leu Tyr Val Ser Pro Ala Val 
            740                 745                 750         


Lys Arg Ser Ile Trp Gln Val Val Arg Ile Val Glu Glu Leu Lys Lys 
        755                 760                 765             


Ile Ile Gly Ser Glu Pro Lys Arg Ile Phe Val Glu Thr Thr Arg Ser 
    770                 775                 780                 


Asn Lys Glu Lys Lys Gly Asp Asp Gly Arg Lys Asp Ser Arg Lys Lys 
785                 790                 795                 800 


Ala Leu Leu Glu Leu Tyr Lys Lys Ile Lys Thr Ser Asp Ile Val Glu 
                805                 810                 815     


Leu Glu Lys Ala Leu Gly His Thr Val Asp Phe Asp Lys Lys Lys Gln 
            820                 825                 830         


Asp Leu Thr Asp Ile Asp Asn Asn Lys Leu Lys Ala Arg Lys Leu Tyr 
        835                 840                 845             


Leu Tyr Tyr Thr Gln Leu Gly Arg Cys Leu Tyr Thr Gly Lys Ala Ile 
    850                 855                 860                 


Asp Leu Asn Asn Leu Leu Ser Ser Asn Ser Glu Tyr Asp Val Asp His 
865                 870                 875                 880 


Ile Tyr Pro Gln Ser Lys Thr Lys Asp Gly Ser Trp Asn Asn Leu Val 
                885                 890                 895     


Leu Val Asn Lys Thr Glu Asn Ser Lys Lys Ser Asn Gly Leu Ile Ser 
            900                 905                 910         


Glu Ser Ile Gln Arg Lys Asn Lys His Phe Trp Arg Phe Leu Lys Ala 
        915                 920                 925             


Lys Asn Leu Ile Ser Glu Glu Lys Tyr Lys Arg Leu Thr Arg Thr Glu 
    930                 935                 940                 


Asp Phe Thr Asp Asp Glu Leu Ser Gly Phe Ile Ala Arg Gln Leu Ile 
945                 950                 955                 960 


Glu Thr Ser Gln Ser Val Lys Ala Val Ser Gln Ile Leu Gln Asn Leu 
                965                 970                 975     


Asn Pro Thr Thr Glu Ile Cys Phe Ser Lys Ala Glu Asn Val His Asp 
            980                 985                 990         


Phe Arg Gln Glu Phe Gly Lys Ile  Lys Val Gly Lys Arg  Arg Asn Leu 
        995                 1000                 1005             


Thr Glu  Glu Gln Gln Lys Glu  Ala Lys Glu Gly Gln  Leu Ile Lys 
    1010                 1015                 1020             


Val Arg  Asp Leu Asn Asp Leu  His His Ala Lys Asp  Ala Tyr Leu 
    1025                 1030                 1035             


Asn Ile  Val Val Gly Asn Val  Tyr His Ile Lys Phe  Thr Arg Asn 
    1040                 1045                 1050             


Pro Lys  Asn Phe Ile Arg Lys  Asn Ser Lys Asn Arg  Glu Tyr Thr 
    1055                 1060                 1065             


Leu Asn  Thr Leu Phe Tyr Ser  Asp Lys Ile Glu Arg  Asn Gly Glu 
    1070                 1075                 1080             


Val Ala  Trp Glu Lys Gly Lys  Thr Glu Lys Ile Val  Ser Ser Val 
    1085                 1090                 1095             


Met Cys  Asn Asn Leu Ile Asn  Val Thr Arg Arg Thr  Ser Glu Gln 
    1100                 1105                 1110             


Lys Gly  Ala Leu Tyr Asp Ala  Thr Ile Tyr Lys Ala  Asn Ala Ala 
    1115                 1120                 1125             


Lys Glu  Gly Ser Tyr Tyr Pro  Val Lys Thr Lys Asn  Lys Asn Leu 
    1130                 1135                 1140             


Lys Asp  Val Thr Arg Tyr Gly  Gly Phe Thr Ser Ile  Lys Ile Ala 
    1145                 1150                 1155             


Tyr Tyr  Ser Ile Phe Lys Tyr  Asp Ile Arg Asn Lys  Asp Lys Val 
    1160                 1165                 1170             


Lys Glu  Asn Ile Lys Ile Ile  Pro Ile Pro Ile Tyr  Ile Ser Gln 
    1175                 1180                 1185             


Asn Ile  Lys Thr Asp Leu Asp  Leu Ile Asn Phe Ala  Glu Lys Gln 
    1190                 1195                 1200             


Ile Lys  Leu Lys Lys Gly Glu  Asn Ile Glu Asn Leu  Gln Leu Ile 
    1205                 1210                 1215             


Tyr Lys  Lys Leu Asn Ile Gly  Ala Leu Val Lys Ile  Asp Tyr Tyr 
    1220                 1225                 1230             


Pro Tyr  Tyr Val Gly Gly Lys  Thr Asn Asp Asn Phe  Tyr Ile Asp 
    1235                 1240                 1245             


Gly Ala  Val Gln Val Leu Leu  Ser Ser Asp Ile Glu  Arg Tyr Leu 
    1250                 1255                 1260             


Lys Glu  Val Val Lys Phe Val  Asn Phe Gln Arg Glu  Asn Lys Glu 
    1265                 1270                 1275             


Ala Lys  Val Ser Lys Glu His  Ile Ser Ser Glu Glu  Asn Ile Glu 
    1280                 1285                 1290             


Leu Tyr  Asn Thr Leu Leu Cys  Lys Met Thr Ala Lys  Ile Phe Thr 
    1295                 1300                 1305             


His Thr  Lys Asn Asn Lys Tyr  Asn Asp Leu Ala Gln  Asp Lys Ala 
    1310                 1315                 1320             


Lys Asn  Thr Phe Lys Asn Leu  Ser Leu Glu Glu Gln  Cys Lys Val 
    1325                 1330                 1335             


Leu Leu  Glu Ile Leu Asn Leu  Leu Thr Asn Lys Lys  Thr Thr Phe 
    1340                 1345                 1350             


Asp Leu  Ser Leu Ile Asn Glu  Lys Ala Ser Arg Val  Lys Cys Gly 
    1355                 1360                 1365             


Phe Asn  Leu Leu Asn Gln Lys  Gln Phe Ser Ile Ile  Glu Gln Ser 
    1370                 1375                 1380             


Val Thr  Gly Leu Tyr Glu Lys  Glu Ile Lys Ile Ile  Glu Asp 
    1385                 1390                 1395         


<210>  1170
<211>  1398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1170

Met Glu Thr Gln Ala Ser Asn Gln Leu Ile Ser Ser His Leu Lys Gly 
1               5                   10                  15      


Tyr Pro Ile Lys Asp Tyr Phe Val Gly Leu Asp Ile Gly Thr Ser Ser 
            20                  25                  30          


Val Gly Trp Ala Val Thr Asn Lys Ala Tyr Glu Leu Leu Lys Phe Arg 
        35                  40                  45              


Ser His Lys Met Trp Gly Ser Arg Leu Phe Asp Glu Gly Glu Ser Ala 
    50                  55                  60                  


Val Ala Arg Arg Gly Phe Arg Ser Met Arg Arg Arg Leu Glu Arg Arg 
65                  70                  75                  80  


Lys Leu Arg Leu Lys Leu Leu Glu Glu Leu Phe Ala Asp Ala Met Ala 
                85                  90                  95      


Gln Val Asp Pro Thr Phe Phe Met Arg Leu Arg Glu Ser Lys Tyr His 
            100                 105                 110         


Tyr Glu Asp Lys Thr Thr Gly His Ser Ser Lys His Ile Leu Phe Ile 
        115                 120                 125             


Asp Lys Asn Tyr Asn Asp Gln Asp Tyr Phe Lys Glu Tyr Pro Thr Val 
    130                 135                 140                 


Tyr His Leu Arg Ser Glu Leu Met Lys Ser Gly Thr Asp Asp Ile Arg 
145                 150                 155                 160 


Lys Leu Phe Leu Ala Val His His Ile Leu Lys Tyr Arg Gly Asn Phe 
                165                 170                 175     


Leu Tyr Glu Gly Ala Thr Phe Asp Ser Asn Ala Ser Thr Leu Asp Asp 
            180                 185                 190         


Val Ile Lys Gln Ala Leu Glu Asn Ile Thr Phe Asn Cys Phe Asp Cys 
        195                 200                 205             


Asn Ser Ala Ile Ser Ser Ile Gly Gln Ile Leu Met Glu Ala Gly Lys 
    210                 215                 220                 


Thr Lys Ser Asp Lys Ala Lys Ala Ile Glu His Leu Val Asp Thr Tyr 
225                 230                 235                 240 


Ile Ala Thr Asp Thr Val Asp Thr Ser Ser Lys Thr Gln Lys Asp Gln 
                245                 250                 255     


Val Lys Glu Asp Lys Lys Arg Leu Lys Ala Phe Ala Asn Leu Val Leu 
            260                 265                 270         


Gly Leu Asn Ala Ser Leu Ile Asp Leu Phe Gly Ser Val Glu Glu Leu 
        275                 280                 285             


Glu Glu Asp Leu Lys Lys Leu Gln Ile Thr Gly Asp Thr Tyr Asp Asp 
    290                 295                 300                 


Lys Arg Asp Glu Leu Ala Lys Ala Trp Ser Asp Glu Ile Tyr Ile Ile 
305                 310                 315                 320 


Asp Asp Cys Lys Ser Val Tyr Asp Ala Ile Ile Leu Leu Ser Ile Lys 
                325                 330                 335     


Glu Pro Gly Leu Thr Ile Ser Glu Ser Lys Val Lys Ala Phe Asn Lys 
            340                 345                 350         


His Lys Asp Asp Leu Ala Ile Leu Lys Ser Leu Leu Lys Ser Asp Arg 
        355                 360                 365             


Ser Ile Tyr Asn Thr Met Phe Lys Val Asp Glu Lys Gly Leu His Asn 
    370                 375                 380                 


Tyr Val His Tyr Ile Lys Gln Gly Arg Thr Glu Glu Thr Ser Cys Asn 
385                 390                 395                 400 


Arg Glu Asp Phe Tyr Lys Tyr Thr Lys Lys Ile Val Glu Gly Leu Ser 
                405                 410                 415     


Asp Ser Lys Asp Lys Glu Tyr Ile Leu Ser Gln Ile Glu Leu Gln Ile 
            420                 425                 430         


Leu Leu Pro Leu Gln Arg Ile Lys Asp Asn Gly Val Ile Pro Tyr Gln 
        435                 440                 445             


Leu His Leu Glu Glu Leu Lys Ala Ile Leu Ala Lys Cys Gly Pro Lys 
    450                 455                 460                 


Phe Pro Phe Leu Asn Glu Val Ala Asp Gly Phe Ser Val Ala Glu Lys 
465                 470                 475                 480 


Leu Ile Lys Met Leu Glu Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu 
                485                 490                 495     


Asn Thr His His Asn Val Asp Asn Gly Gly Phe Ala Trp Ala Val Arg 
            500                 505                 510         


Lys Ala Ser Gly Arg Val Thr Pro Trp Asn Phe Asp Asp Lys Ile Asp 
        515                 520                 525             


Arg Glu Lys Ser Ala Ala Ala Phe Ile Lys Asn Leu Thr Asn Lys Cys 
    530                 535                 540                 


Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Lys Ser Ser Leu Leu Tyr 
545                 550                 555                 560 


Ser Glu Phe Met Leu Leu Asn Glu Leu Asn Asn Val Arg Ile Asp Gly 
                565                 570                 575     


Lys Pro Leu Glu Lys Val Val Lys Glu His Leu Ile Glu Ala Val Phe 
            580                 585                 590         


Lys Gln Asp His Lys Lys Met Thr Lys Asn Arg Ile Glu Gln Phe Leu 
        595                 600                 605             


Lys Asp Asn Gly Tyr Ile Ser Glu Thr His Lys His Glu Ile Thr Gly 
    610                 615                 620                 


Leu Asp Gly Glu Ile Lys Asn Asp Leu Ala Ser Tyr Arg Asp Met Val 
625                 630                 635                 640 


Arg Ile Leu Gly Asp Gly Phe Asp Arg Ser Met Ala Glu Glu Ile Ile 
                645                 650                 655     


Thr Asp Ile Thr Ile Phe Gly Glu Ser Lys Lys Met Leu Arg Glu Thr 
            660                 665                 670         


Leu Arg Lys Lys Phe Ala Ser Cys Leu Asp Asp Glu Ala Ile Lys Lys 
        675                 680                 685             


Leu Thr Lys Leu Arg Tyr Arg Asp Trp Gly Arg Leu Ser Gln Lys Leu 
    690                 695                 700                 


Leu Asn Gly Ile Glu Gly Cys Asp Lys Ala Gly Asp Gly Thr Pro Glu 
705                 710                 715                 720 


Thr Ile Ile Ile Leu Met Arg Asn Phe Ser Tyr Asn Leu Met Glu Leu 
                725                 730                 735     


Leu Gly Asp Lys Phe Ser Phe Met Glu Arg Ile Gln Glu Ile Asn Ala 
            740                 745                 750         


Lys Leu Thr Glu Gly Gln Ile Val Asn Pro His Asp Ile Ile Asp Asp 
        755                 760                 765             


Leu Ala Leu Ser Pro Ala Val Lys Arg Ala Val Trp Gln Ala Leu Arg 
    770                 775                 780                 


Ile Val Asp Glu Val Ala His Ile Lys Lys Ala Leu Pro Ala Arg Ile 
785                 790                 795                 800 


Phe Val Glu Val Thr Arg Ser Asn Lys Asn Glu Lys Lys Lys Lys Asp 
                805                 810                 815     


Ser Arg Gln Lys Arg Leu Ser Asp Leu Tyr Ala Ala Ile Lys Lys Asp 
            820                 825                 830         


Asp Val Leu Leu Asn Gly Leu Asn Asn Glu Ile Phe Gly Glu Leu Lys 
        835                 840                 845             


Ser Ser Leu Ala Lys Tyr Asp Asp Ala Ala Leu Arg Ser Lys Lys Leu 
    850                 855                 860                 


Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Cys Ala Tyr Thr Gly Glu Ile 
865                 870                 875                 880 


Ile Glu Leu Ser Leu Leu Asn Thr Asp Asn Tyr Asp Ile Asp His Ile 
                885                 890                 895     


Tyr Pro Arg Ser Leu Thr Lys Asp Asp Ser Phe Asp Asn Leu Val Leu 
            900                 905                 910         


Cys Lys Arg Thr Ala Asn Ala Gln Lys Ser Asp Ala Tyr Pro Ile Ser 
        915                 920                 925             


Glu Glu Ile Gln Lys Thr Gln Lys Pro Phe Trp Thr Phe Leu Lys Gln 
    930                 935                 940                 


Gln Gly Leu Ile Ser Glu Arg Lys Tyr Glu Arg Leu Thr Arg Ile Thr 
945                 950                 955                 960 


Pro Leu Thr Ala Asp Asp Leu Ser Gly Phe Ile Ala Arg Gln Leu Val 
                965                 970                 975     


Glu Thr Asn Gln Ser Val Lys Ala Ala Thr Thr Leu Leu Arg Arg Leu 
            980                 985                 990         


Tyr Pro Gly Val Asp Val Val Phe  Val Lys Ala Glu Asn  Val Thr Asp 
        995                 1000                 1005             


Phe Arg  His Asp Asn Asn Phe  Ile Lys Val Arg Ser  Leu Asn His 
    1010                 1015                 1020             


His His  His Ala Lys Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn 
    1025                 1030                 1035             


Val Tyr  His Glu Arg Phe Thr  Arg Asn Phe Arg Ala  Phe Phe Lys 
    1040                 1045                 1050             


Lys Asn  Gly Ala Asn Arg Thr  Tyr Asn Leu Ala Lys  Met Phe Asn 
    1055                 1060                 1065             


Tyr Asp  Val Asn Cys Thr Asn  Ala Lys Asp Gly Lys  Ala Trp Asp 
    1070                 1075                 1080             


Val Lys  Thr Ser Met Asp Thr  Val Lys Lys Met Met  Asp Ser Asn 
    1085                 1090                 1095             


Asp Val  Arg Val Thr Lys Arg  Leu Leu Glu Gln Thr  Gly Ala Leu 
    1100                 1105                 1110             


Ala Asp  Ala Thr Ile Tyr Lys  Ala Thr Val Ala Gly  Lys Ala Lys 
    1115                 1120                 1125             


Asp Gly  Ala Tyr Ile Gly Met  Lys Thr Lys Ser Ser  Val Phe Ala 
    1130                 1135                 1140             


Asp Val  Ser Lys Tyr Gly Gly  Met Thr Lys Ile Lys  Asn Ala Tyr 
    1145                 1150                 1155             


Ser Ile  Ile Val Gln Tyr Thr  Gly Lys Lys Gly Glu  Val Ile Lys 
    1160                 1165                 1170             


Glu Ile  Val Pro Leu Pro Ile  Tyr Leu Thr Asn Arg  Asn Thr Thr 
    1175                 1180                 1185             


Asp Gln  Asp Leu Ile Asn Tyr  Val Ala Ser Ile Ile  Pro Gln Ala 
    1190                 1195                 1200             


Lys Asp  Ile Ser Ile Ile Tyr  Gly Lys Leu Cys Ile  Asn Gln Leu 
    1205                 1210                 1215             


Val Lys  Val Asn Gly Phe Tyr  Tyr Tyr Leu Gly Gly  Lys Thr Asn 
    1220                 1225                 1230             


Ser Lys  Phe Cys Ile Asp Asn  Ala Ile Gln Val Ile  Val Ser Asn 
    1235                 1240                 1245             


Glu Trp  Ile Pro Tyr Leu Lys  Val Leu Glu Lys Phe  Asn Asn Met 
    1250                 1255                 1260             


Arg Lys  Asp Asn Lys Asp Leu  Lys Ala Asn Val Val  Ser Thr Arg 
    1265                 1270                 1275             


Ala Leu  Asp Asn Lys His Thr  Ile Glu Val Arg Ile  Val Glu Glu 
    1280                 1285                 1290             


Lys Asn  Ile Glu Phe Phe Asp  Tyr Leu Val Ser Lys  Leu Lys Met 
    1295                 1300                 1305             


Pro Ile  Tyr Gln Lys Met Lys  Gly Asn Lys Ala Ala  Glu Leu Ser 
    1310                 1315                 1320             


Glu Lys  Gly Tyr Gly Leu Phe  Lys Lys Met Ser Leu  Glu Glu Gln 
    1325                 1330                 1335             


Ser Ile  His Leu Ile Glu Leu  Leu Asn Leu Leu Thr  Asn Gln Lys 
    1340                 1345                 1350             


Thr Thr  Phe Glu Val Lys Pro  Leu Gly Ile Thr Ala  Ser Arg Ser 
    1355                 1360                 1365             


Thr Val  Gly Ser Lys Ile Ser  Asn Gln Asp Glu Phe  Lys Val Ile 
    1370                 1375                 1380             


Asn Glu  Ser Ile Thr Gly Leu  Tyr Ser Asn Glu Val  Thr Ile Val 
    1385                 1390                 1395             


<210>  1171
<211>  1398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1171

Met Glu Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ser Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Arg Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ala Lys Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         


Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             


His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 


Ser Gln Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Tyr Asp Asn Phe Asn Asp Asp 
                165                 170                 175     


Asn Phe Asp Trp Arg Asn Ile Asp Ile Gln Lys Arg Tyr Glu Glu Phe 
            180                 185                 190         


Ile Glu Thr Tyr Asp Ser Thr Leu Gly Glu Ser Tyr Leu Ala Asp Ile 
        195                 200                 205             


Ser Val Asp Ala Ala Ser Ile Leu Glu Glu Lys Val Ser Lys Thr Glu 
    210                 215                 220                 


Arg Leu Glu Asn Leu Leu Lys Tyr Tyr Pro Thr Glu Lys Lys Thr Thr 
225                 230                 235                 240 


Phe Phe Gly Asn Leu Ile Lys Leu Ile Leu Gly Gln Gln Ala Lys Phe 
                245                 250                 255     


Lys Ala Ile Phe Asn Leu Glu Asp Glu Ile Ser Leu Gln Phe Ala Thr 
            260                 265                 270         


Pro Thr Tyr Asp Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Asp Asn 
        275                 280                 285             


Gly Asp Ser Tyr Ser Glu Leu Phe Val Ala Ala Gln Asn Leu Tyr Asn 
    290                 295                 300                 


Thr Ile Leu Leu Ala Ser Phe Leu Lys Thr Asp Asn Lys Ser Ala Lys 
305                 310                 315                 320 


Ala Pro Leu Ser Thr Ser Met Ile Glu Arg Tyr Glu Asn His Lys Lys 
                325                 330                 335     


Asp Leu Ala Lys Leu Lys Asp Phe Val Lys Lys Asn Cys Pro Asp Gln 
            340                 345                 350         


Tyr His Asp Ile Phe Arg Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Asn Gly Val Ser Gln Asn Asp Phe Tyr Thr Phe Ile Gly Lys 
    370                 375                 380                 


Cys Leu Glu Glu Ser Leu Lys Lys Asp Lys Gly Ala Gln Tyr Phe Leu 
385                 390                 395                 400 


Asp Lys Ile Asp Arg Asp Asp Phe Leu Arg Lys Gln Arg Thr Phe Asp 
                405                 410                 415     


Asn Gly Ala Phe Pro Tyr Gln Ile His Leu Gln Glu Met His Ala Ile 
            420                 425                 430         


Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe Leu Lys Glu Asn Gln Asp 
        435                 440                 445             


Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro 
    450                 455                 460                 


Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp Ala Asn Tyr Arg Ser Asp 
465                 470                 475                 480 


Glu Ala Ile Thr Pro Trp Asn Phe Asp Glu Val Val Asp Lys Glu Lys 
                485                 490                 495     


Ser Ala Glu Lys Phe Ile Thr Arg Met Thr Leu Asn Asp Leu Tyr Leu 
            500                 505                 510         


Pro Glu Glu Lys Val Leu Pro Lys His Ser Leu Ile Tyr Glu Thr Phe 
        515                 520                 525             


Thr Val Tyr Asn Glu Leu Thr Asn Ile Lys Tyr Val Asn Asp Gln Gly 
    530                 535                 540                 


Asn Ala Ile His Phe Asp Ser Glu Leu Lys Glu Lys Ile Phe Asn Gln 
545                 550                 555                 560 


Leu Phe Lys Glu Asn Arg Lys Val Ser Lys Lys Thr Leu Ile Asp Phe 
                565                 570                 575     


Leu Asn Asn Ser Glu Gly Ile Tyr Thr Asp Lys Leu Val Gly Ile Asp 
            580                 585                 590         


Glu Glu Val Lys Tyr Leu Asn Ala Ser Leu Gly Thr Tyr His Asp Leu 
        595                 600                 605             


Lys Lys Ile Leu Glu Ser Phe Met Asp Asp Glu Ile Asn Glu Lys Ile 
    610                 615                 620                 


Ile Glu Asp Ile Ile Gln Thr Leu Thr Leu Phe Glu Asp Ile Glu Met 
625                 630                 635                 640 


Lys Arg Gln Arg Leu Gln Lys Tyr Asp Asp Ile Phe Thr Pro Lys Gln 
                645                 650                 655     


Leu Lys Glu Leu Ala Arg Arg Asn Tyr Thr Gly Trp Gly Arg Leu Ser 
            660                 665                 670         


Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys Glu Asn Asn Lys Thr Ile 
        675                 680                 685             


Leu Asp Tyr Leu Lys Asn Gly Asn Arg Asn Phe Met Gln Leu Ile Asn 
    690                 695                 700                 


Asp Asp Arg Leu Ser Phe Lys Gln Ile Ile Ile Asp Ala Arg Lys Ile 
705                 710                 715                 720 


Glu Lys Leu Asp Asn Ile Glu Ser Val Val Tyr Asn Leu Pro Gly Ser 
                725                 730                 735     


Pro Ala Ile Lys Lys Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu 
            740                 745                 750         


Leu Val Lys Val Met Gly His Asn Pro Asp Asn Ile Val Ile Glu Met 
        755                 760                 765             


Ala Arg Glu Asn Gln Thr Thr Asn Gln Gly Lys Asn Arg Ser Gln Gln 
    770                 775                 780                 


Arg Leu Lys Arg Leu Gln Asp Ser Met Ser Asn Phe Lys Asp Ser Ser 
785                 790                 795                 800 


Ile Ser Leu Lys Asp Val Asp Asn Ser Asp Leu Gln Asn Asp Arg Leu 
                805                 810                 815     


Phe Leu Tyr Tyr Ile Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu 
            820                 825                 830         


Leu Asp Ile Asp His Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro 
        835                 840                 845             


Gln Ser Phe Ile Lys Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser 
    850                 855                 860                 


Ser Ala Lys Asn Arg Gly Lys Ser Asp Asp Val Pro Gly Arg Asp Val 
865                 870                 875                 880 


Val Leu Lys Met Lys Pro Phe Trp Lys Lys Leu Tyr Asp Val Lys Leu 
                885                 890                 895     


Ile Ser Lys Arg Lys Phe Asp Asn Leu Thr Lys Ser Glu His Gly Gly 
            900                 905                 910         


Leu Thr Glu Ser Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu 
        915                 920                 925             


Thr Arg Gln Ile Thr Lys Tyr Val Ala Gln Ile Leu Asp Gly Arg Phe 
    930                 935                 940                 


Asn Thr Lys Arg Asp Asp Asn Asn Lys Val Ile Arg Asp Val Lys Val 
945                 950                 955                 960 


Ile Thr Leu Lys Ser Ser Leu Val Ser Gln Phe Arg Lys Asp Phe Gly 
                965                 970                 975     


Phe Tyr Lys Val Arg Glu Ile Asn Asp Tyr His His Ala His Asp Ala 
            980                 985                 990         


Tyr Leu Asn Ala Val Val Gly Thr  Ala Ile Leu Lys Lys  Tyr Pro Lys 
        995                 1000                 1005             


Leu Ala  Pro Glu Phe Val Tyr  Gly Glu Tyr Lys Lys  Cys Asp Val 
    1010                 1015                 1020             


Arg Lys  Leu Ile Ala Lys Ser  Gly Asp Lys Ser Glu  Ile Gly Lys 
    1025                 1030                 1035             


Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn Leu Met  Asn Phe Phe 
    1040                 1045                 1050             


Lys Arg  Val Ile Arg Tyr Ser  Asn Gly Met Ile Val  Val Arg Pro 
    1055                 1060                 1065             


Val Ile  Glu Tyr Ser Lys Asp  Thr Gly Glu Ile Ala  Trp Asp Lys 
    1070                 1075                 1080             


Glu Lys  Asp Phe Lys Thr Val  Cys Lys Val Leu Ser  Cys Pro Gln 
    1085                 1090                 1095             


Val Asn  Ile Val Lys Lys Val  Glu Lys Gln Ser His  Gly Leu Asp 
    1100                 1105                 1110             


Arg Gly  Lys Pro Lys Gly Phe  Tyr Asn Ala Asn Pro  Ser Pro Lys 
    1115                 1120                 1125             


Pro Lys  Lys Gly Ser Lys Val  Asn Leu Val Pro Ile  Lys Ala Asn 
    1130                 1135                 1140             


Leu Asn  Pro Lys Asn Tyr Gly  Gly Tyr Ala Gly Ile  Ser Asn Ser 
    1145                 1150                 1155             


Tyr Ala  Val Leu Val Asp Ala  Thr Ile Glu Lys Gly  Ala Lys Lys 
    1160                 1165                 1170             


Lys Leu  Thr Arg Ile Gln Glu  Phe Gln Gly Ile Ser  Ile Ile Asp 
    1175                 1180                 1185             


Arg Glu  Lys Tyr Glu Lys Asn  Lys Val Glu Phe Leu  Lys Gly Leu 
    1190                 1195                 1200             


Gly Tyr  Lys Glu Ile Tyr Ser  Ile Ile Thr Leu Pro  Lys Tyr Ser 
    1205                 1210                 1215             


Leu Phe  Glu Leu Ala Asp Gly  Ser Arg Arg Met Leu  Ala Ser Ile 
    1220                 1225                 1230             


Leu Ser  Thr Asn Asn Lys Arg  Gly Glu Ile His Lys  Gly Asn Glu 
    1235                 1240                 1245             


Leu Val  Leu Pro Ala Lys Tyr  Ile Pro Leu Leu Tyr  His Ala Asn 
    1250                 1255                 1260             


Arg Ile  His Asn Thr Phe Glu  Thr Gly His Arg Glu  Tyr Val Glu 
    1265                 1270                 1275             


Lys His  Ile Ala Glu Phe Lys  Glu Ile Ala Glu Ile  Ile Leu Glu 
    1280                 1285                 1290             


Phe Asn  Asn Lys Tyr Val Asn  Ala Lys Lys Asn Ser  Ser Ile Ile 
    1295                 1300                 1305             


Glu Lys  Ala Leu Glu Ser Phe  Asp Ser Phe Ser Leu  Asp Glu Ile 
    1310                 1315                 1320             


Cys Asp  Ser Phe Val Gly Lys  Leu Lys Lys Asn Asn  Thr Lys Lys 
    1325                 1330                 1335             


Asn Ser  Gly Leu Phe Glu Leu  Val Ser Leu Gly Ser  Ala Ser Asp 
    1340                 1345                 1350             


Phe Glu  Phe Leu Glu Thr Lys  Val Pro Arg Tyr Arg  Asp Tyr Thr 
    1355                 1360                 1365             


Pro Ser  Ser Leu Leu Asn Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1370                 1375                 1380             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Lys Leu  Gly Glu Glu 
    1385                 1390                 1395             


<210>  1172
<211>  1398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1172

Met Glu Thr Gln Ala Ser Asn Gln Leu Ile Ser Ser His Leu Lys Gly 
1               5                   10                  15      


Tyr Pro Ile Lys Asp Tyr Phe Val Gly Leu Asp Ile Gly Thr Ser Ser 
            20                  25                  30          


Val Gly Trp Ala Val Thr Asn Lys Ala Tyr Glu Leu Leu Lys Phe Arg 
        35                  40                  45              


Ser His Lys Met Trp Gly Ser Arg Leu Phe Asp Glu Gly Glu Ser Ala 
    50                  55                  60                  


Val Ala Arg Arg Gly Phe Arg Ser Met Arg Arg Arg Leu Glu Arg Arg 
65                  70                  75                  80  


Lys Leu Arg Leu Lys Leu Leu Glu Glu Leu Phe Ala Asp Ala Met Ala 
                85                  90                  95      


Gln Val Asp Pro Thr Phe Phe Met Arg Leu Arg Glu Ser Lys Tyr His 
            100                 105                 110         


Tyr Glu Asp Lys Thr Thr Gly His Ser Ser Lys His Ile Leu Phe Ile 
        115                 120                 125             


Asp Lys Asn Tyr Thr Asp Gln Asp Tyr Tyr Lys Ala Tyr Pro Thr Ile 
    130                 135                 140                 


Tyr His Leu Arg Ala Glu Leu Met Lys Asp Gly Thr Tyr Asp Ile Arg 
145                 150                 155                 160 


Lys Leu Phe Leu Ala Val His His Ile Leu Lys Tyr Arg Gly Asn Phe 
                165                 170                 175     


Leu Tyr Glu Gly Ala Ser Phe Asp Ser Asn Ala Ser Thr Leu Asp Asp 
            180                 185                 190         


Val Leu Lys Gln Ala Leu Glu Asn Ile Thr Phe Asn Cys Phe Asp Ser 
        195                 200                 205             


Ser Ala Ala Ile Gly Ser Ile Gly Gln Ile Leu Met Glu Thr Gly Lys 
    210                 215                 220                 


Thr Lys Ser Asp Lys Ala Lys Thr Ile Glu Arg Leu Val Asp Thr Tyr 
225                 230                 235                 240 


Ile Ile Thr Asp Glu Val Asn Ala Ser Gly Lys Thr Gln Lys Asp Gln 
                245                 250                 255     


Val Lys Glu Asp Lys Lys Arg Leu Lys Ala Phe Ala Asn Leu Val Leu 
            260                 265                 270         


Gly Leu Asn Ala Ser Leu Ile Asp Leu Phe Gly Ser Val Glu Glu Leu 
        275                 280                 285             


Glu Glu Asp Leu Lys Lys Leu Gln Ile Thr Gly Asp Thr Tyr Asp Asp 
    290                 295                 300                 


Lys Arg Asp Glu Leu Ala Lys Ala Trp Ser Asp Glu Ile Tyr Ile Ile 
305                 310                 315                 320 


Asp Asp Cys Lys Ser Val Tyr Asp Ala Ile Ile Leu Leu Ser Ile Lys 
                325                 330                 335     


Glu Pro Gly Leu Thr Ile Ser Glu Ser Lys Val Lys Ala Phe Asn Lys 
            340                 345                 350         


His Lys Asp Asp Leu Ala Ile Leu Lys Ser Leu Leu Lys Ser Asp Arg 
        355                 360                 365             


Ser Ile Tyr Asn Ala Met Phe Lys Val Asp Glu Lys Gly Leu His Asn 
    370                 375                 380                 


Tyr Val His Tyr Ile Lys Gln Gly Arg Thr Glu Glu Thr Ser Cys Asn 
385                 390                 395                 400 


Arg Glu Asp Phe Tyr Lys Tyr Thr Lys Lys Val Val Glu Gly Leu Pro 
                405                 410                 415     


Asp Ser Lys Asp Lys Glu Tyr Ile Leu Ser Glu Ile Glu Leu Gln Thr 
            420                 425                 430         


Leu Leu Pro Leu Gln Arg Ile Lys Asp Asn Gly Val Ile Pro Tyr Gln 
        435                 440                 445             


Leu His Leu Glu Glu Leu Lys Val Ile Leu Ala Lys Cys Gly Pro Lys 
    450                 455                 460                 


Phe Pro Phe Leu Asn Glu Val Ala Asp Gly Phe Ser Val Ala Glu Lys 
465                 470                 475                 480 


Leu Ile Lys Met Leu Glu Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu 
                485                 490                 495     


Asn Thr Ser His Asn Val Asp Asn Gly Gly Phe Ala Trp Ala Val Arg 
            500                 505                 510         


Lys Ala Ser Gly Arg Val Thr Pro Trp Asn Phe Asp Asp Lys Ile Asp 
        515                 520                 525             


Arg Glu Lys Ser Ala Ala Ala Phe Ile Lys Asn Leu Thr Asn Lys Cys 
    530                 535                 540                 


Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Lys Ser Ser Leu Leu Tyr 
545                 550                 555                 560 


Ser Glu Phe Met Leu Leu Asn Glu Leu Asn Asn Val Arg Ile Asp Gly 
                565                 570                 575     


Lys Pro Leu Glu Thr Lys Val Lys Ala His Leu Ile Lys Ala Val Phe 
            580                 585                 590         


Lys Gln Asp His Lys Lys Met Thr Lys Asn Arg Ile Glu Gln Phe Leu 
        595                 600                 605             


Lys Asp Asn Asn Tyr Ile Pro Glu Lys His Lys Pro Glu Ile Thr Gly 
    610                 615                 620                 


Leu Asp Gly Glu Ile Lys Asn Asp Leu Ala Ser Tyr Arg Asp Met Val 
625                 630                 635                 640 


Arg Ile Leu Gly Asn Asn Phe Asp Leu Ser Met Ala Glu Glu Ile Ile 
                645                 650                 655     


Thr Asp Ile Thr Ile Phe Gly Glu Ser Lys Lys Met Leu Arg Glu Thr 
            660                 665                 670         


Leu Arg Lys Lys Phe Ala Ser Cys Leu Asp Asp Glu Ala Ile Lys Lys 
        675                 680                 685             


Leu Ser Lys Leu Arg Tyr Arg Asp Trp Gly Arg Leu Ser Lys Lys Leu 
    690                 695                 700                 


Leu Asn Gly Ile Asp Gly Cys Asp Lys Ala Gly Asp Gly Thr Ser Val 
705                 710                 715                 720 


Thr Ile Ile Glu Ala Met Arg Asp Phe Ser Tyr Asn Leu Met Glu Leu 
                725                 730                 735     


Leu Gly Asp Arg Phe Leu Phe Met Glu Arg Ile Gln Glu Ile Asn Asp 
            740                 745                 750         


Lys Leu Thr Glu Gly Gln Ile Val Asn Pro His Asp Ile Ile Asp Asp 
        755                 760                 765             


Leu Ala Leu Ser Pro Ala Val Lys Arg Ala Val Trp Gln Ala Leu Arg 
    770                 775                 780                 


Ile Val Asp Glu Ile Val His Ile Lys Lys Ala Leu Pro Ala Arg Ile 
785                 790                 795                 800 


Phe Val Glu Val Thr Arg Ser Asn Lys Ala Glu Lys Lys Lys Lys Asp 
                805                 810                 815     


Ser Arg Gln Lys Arg Leu Ser Asp Leu Tyr Ala Ala Ile Lys Lys Asp 
            820                 825                 830         


Asp Ala Leu Leu Ser Gly Leu Thr Asn Glu Glu Phe Gly Gly Leu Lys 
        835                 840                 845             


Ser Glu Leu Ala Lys Tyr Asp Asp Ala Ala Leu Arg Ser Lys Lys Leu 
    850                 855                 860                 


Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Cys Ala Tyr Thr Gly Glu Val 
865                 870                 875                 880 


Ile Asp Leu Ser Gln Leu Asn Thr Asp Asn Tyr Asp Ile Asp His Ile 
                885                 890                 895     


Tyr Pro Arg Ser Leu Thr Lys Asp Asp Ser Phe Asp Asn Leu Val Leu 
            900                 905                 910         


Cys Lys Arg Thr Ala Asn Ala Gln Lys Ser Asp Thr Tyr Pro Ile Ser 
        915                 920                 925             


Lys Glu Ile Gln Lys Ala Gln Lys Ala Phe Trp Ile Phe Leu Lys Gln 
    930                 935                 940                 


Gln Gly Leu Ile Ser Glu Arg Lys Tyr Glu Arg Leu Thr Arg Ile Thr 
945                 950                 955                 960 


Pro Leu Thr Ala Asp Asp Leu Ser Gly Phe Ile Ala Arg Gln Leu Val 
                965                 970                 975     


Glu Thr Asn Gln Ser Val Lys Ala Ala Thr Thr Leu Leu Arg Arg Leu 
            980                 985                 990         


Tyr Pro Asp Ile Asp Val Val Phe  Val Lys Ala Glu Asn  Val Thr Asp 
        995                 1000                 1005             


Phe Arg  His Asp Asn Asn Phe  Ile Lys Val Arg Ser  Leu Asn His 
    1010                 1015                 1020             


His His  His Ala Lys Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn 
    1025                 1030                 1035             


Val Tyr  His Glu Lys Phe Thr  Arg Asn Phe Arg Ala  Phe Phe Lys 
    1040                 1045                 1050             


Lys Asn  Gly Ala Asn Arg Thr  Tyr Asn Leu Ala Lys  Met Phe Asn 
    1055                 1060                 1065             


Tyr Asp  Val Lys Cys Thr Asn  Ala Lys Asp Gly Lys  Ala Trp Asp 
    1070                 1075                 1080             


Val Lys  Thr Ser Met Asp Thr  Val Asn Lys Met Met  Thr Ser Asn 
    1085                 1090                 1095             


Asp Val  Arg Val Thr Lys Arg  Leu Leu Glu Gln Ser  Gly Ala Leu 
    1100                 1105                 1110             


Ala Asp  Ala Thr Val Tyr Lys  Ala Thr Val Ala Ala  Lys Ala Lys 
    1115                 1120                 1125             


Asp Gly  Ala Tyr Ile Gly Met  Lys Thr Lys Ser Ser  Val Phe Ala 
    1130                 1135                 1140             


Asp Val  Thr Lys Tyr Gly Gly  Met Thr Lys Ile Lys  Asn Ala Tyr 
    1145                 1150                 1155             


Ser Ile  Ile Val Gln Tyr Ile  Gly Lys Lys Gly Glu  Val Val Lys 
    1160                 1165                 1170             


Glu Ile  Val Pro Leu Pro Ile  Tyr Leu Thr Asn Arg  Asn Ile Thr 
    1175                 1180                 1185             


Asp Gln  Asp Leu Ile Asp Tyr  Val Ala Ser Ile Thr  Pro Gln Ala 
    1190                 1195                 1200             


Lys Asp  Ile Ser Ile Ile Tyr  Arg Lys Leu Cys Ile  Asn Gln Leu 
    1205                 1210                 1215             


Val Lys  Val Asn Gly Phe Tyr  Tyr Tyr Leu Gly Gly  Lys Thr Asn 
    1220                 1225                 1230             


Ser Asn  Ile Tyr Ile Asp Asn  Ala Val Glu Val Val  Val Pro Ala 
    1235                 1240                 1245             


Asn Ile  Ala Val Tyr Ile Lys  Val Leu Glu Lys Phe  Ala Leu Leu 
    1250                 1255                 1260             


His Lys  Asp Asn Asn Asn Ile  Lys Ala Ser Thr Val  Thr Thr Arg 
    1265                 1270                 1275             


Ile Tyr  Asn Glu Gly Ser Thr  Asn Ile Val Ser Ile  Thr Asp Lys 
    1280                 1285                 1290             


Asp Gly  Leu Ile Val Tyr Asp  Tyr Leu Met Ser Lys  Leu Arg Thr 
    1295                 1300                 1305             


Pro Leu  Tyr Met Lys Met Lys  Gly Asn Lys Val Asp  Glu Leu Ser 
    1310                 1315                 1320             


Thr Val  Gly Arg Ser Lys Phe  Met Asn Met Thr Leu  Glu Glu Gln 
    1325                 1330                 1335             


Ser Ile  Tyr Leu Leu Glu Val  Leu Asn Leu Leu Thr  Asn Ser Lys 
    1340                 1345                 1350             


Thr Thr  Phe Asp Val Lys Ala  Leu Gly Ile Thr Ala  Ser Arg Ser 
    1355                 1360                 1365             


Thr Ile  Gly Val Lys Ile His  Thr Leu Asp Glu Phe  Lys Ile Ile 
    1370                 1375                 1380             


Asn Gln  Ser Ile Thr Gly Leu  Tyr Ser Asn Glu Ile  Thr Ile Val 
    1385                 1390                 1395             


<210>  1173
<211>  1398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1173

Met Glu Thr Gln Thr Ser Asn Gln Leu Ile Thr Ser His Leu Lys Asp 
1               5                   10                  15      


Tyr Pro Lys Gln Asp Tyr Phe Val Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Val Thr Asn Thr Ser Tyr Glu Leu Leu Lys Phe His 
        35                  40                  45              


Ser His Lys Met Trp Gly Ser Arg Leu Phe Glu Glu Gly Glu Ser Ala 
    50                  55                  60                  


Val Thr Arg Arg Gly Phe Arg Ser Met Arg Arg Arg Leu Glu Arg Arg 
65                  70                  75                  80  


Lys Leu Arg Leu Lys Leu Leu Glu Glu Leu Phe Ala Asp Ala Met Ala 
                85                  90                  95      


Gln Val Asp Ser Thr Phe Phe Ile Arg Leu His Glu Ser Lys Tyr His 
            100                 105                 110         


Tyr Glu Asp Lys Thr Thr Gly His Ser Ser Lys His Ile Leu Phe Ile 
        115                 120                 125             


Asp Glu Asp Tyr Thr Asp Gln Asp Tyr Phe Thr Glu Tyr Pro Thr Ile 
    130                 135                 140                 


Tyr His Leu Arg Lys Asp Leu Met Glu Asn Gly Thr Asp Asp Ile Arg 
145                 150                 155                 160 


Lys Leu Phe Leu Ala Val His His Ile Leu Lys Tyr Arg Gly Asn Phe 
                165                 170                 175     


Leu Tyr Glu Gly Ala Thr Phe Asn Ser Asn Ala Phe Thr Phe Glu Asp 
            180                 185                 190         


Val Leu Lys Gln Ala Leu Val Asn Ile Thr Phe Asn Cys Phe Asp Thr 
        195                 200                 205             


Asn Ser Ala Ile Ser Ser Ile Ser Asn Ile Leu Met Glu Ser Gly Lys 
    210                 215                 220                 


Thr Lys Ser Asp Lys Ala Lys Ala Ile Glu Arg Leu Val Asp Thr Tyr 
225                 230                 235                 240 


Thr Val Phe Asp Glu Val Asn Thr Pro Asp Lys Pro Gln Lys Glu Gln 
                245                 250                 255     


Val Lys Glu Asp Lys Lys Thr Leu Lys Ala Phe Ala Asn Leu Val Leu 
            260                 265                 270         


Gly Leu Ser Ala Asn Leu Ile Asp Leu Phe Gly Ser Val Glu Asp Ile 
        275                 280                 285             


Asp Asp Asp Leu Lys Lys Leu Gln Ile Val Gly Asp Thr Tyr Asp Glu 
    290                 295                 300                 


Lys Arg Asp Glu Leu Ala Lys Val Trp Gly Asp Glu Ile His Ile Ile 
305                 310                 315                 320 


Asp Asp Cys Lys Ser Val Tyr Asp Ala Ile Ile Leu Met Ser Ile Lys 
                325                 330                 335     


Glu Pro Gly Leu Thr Ile Ser Gln Ser Lys Val Lys Ala Phe Asp Lys 
            340                 345                 350         


His Lys Glu Asp Leu Val Ile Leu Lys Ser Leu Leu Lys Leu Asp Arg 
        355                 360                 365             


Asn Val Tyr Asn Glu Met Phe Lys Ser Asp Lys Lys Gly Leu His Asn 
    370                 375                 380                 


Tyr Val His Tyr Ile Lys Gln Gly Arg Thr Glu Glu Thr Ser Cys Ser 
385                 390                 395                 400 


Arg Glu Asp Phe Tyr Lys Tyr Thr Lys Lys Ile Val Glu Gly Leu Ala 
                405                 410                 415     


Asp Ser Lys Asp Lys Glu Tyr Ile Leu Asn Glu Ile Glu Leu Gln Thr 
            420                 425                 430         


Leu Leu Pro Leu Gln Arg Ile Lys Asp Asn Gly Val Ile Pro Tyr Gln 
        435                 440                 445             


Leu His Leu Glu Glu Leu Lys Val Ile Leu Asp Lys Cys Gly Pro Lys 
    450                 455                 460                 


Phe Pro Phe Leu His Thr Val Ser Asp Gly Phe Ser Val Thr Glu Lys 
465                 470                 475                 480 


Leu Ile Lys Met Leu Glu Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu 
                485                 490                 495     


Asn Thr His His Asn Ile Asp Asn Gly Gly Phe Ser Trp Ala Val Arg 
            500                 505                 510         


Lys Gln Ala Gly Arg Val Thr Pro Trp Asn Phe Glu Glu Lys Ile Asp 
        515                 520                 525             


Arg Glu Lys Ser Ala Ala Ala Phe Ile Lys Asn Leu Thr Asn Lys Cys 
    530                 535                 540                 


Thr Tyr Leu Phe Gly Glu Asp Val Leu Pro Lys Ser Ser Leu Leu Tyr 
545                 550                 555                 560 


Ser Glu Phe Met Leu Leu Asn Glu Leu Asn Asn Val Arg Ile Asp Gly 
                565                 570                 575     


Lys Ala Leu Ala Gln Gly Val Lys Gln His Leu Ile Asp Ser Ile Phe 
            580                 585                 590         


Lys Gln Asp His Lys Lys Met Thr Lys Asn Arg Ile Glu Leu Phe Leu 
        595                 600                 605             


Lys Asp Asn Asn Tyr Ile Thr Lys Lys His Lys Pro Glu Ile Thr Gly 
    610                 615                 620                 


Leu Asp Gly Glu Ile Lys Asn Asp Leu Thr Ser Tyr Arg Asp Met Val 
625                 630                 635                 640 


Arg Ile Leu Gly Asn Asn Phe Asp Val Ser Met Ala Glu Asp Ile Ile 
                645                 650                 655     


Thr Asp Ile Thr Ile Phe Gly Glu Ser Lys Lys Met Leu Arg Gln Thr 
            660                 665                 670         


Leu Arg Asn Lys Phe Gly Ser Gln Leu Asn Asp Glu Thr Ile Lys Lys 
        675                 680                 685             


Leu Ser Lys Leu Arg Tyr Arg Asp Trp Gly Arg Leu Ser Lys Lys Leu 
    690                 695                 700                 


Leu Lys Gly Ile Asp Gly Cys Asp Lys Ala Gly Asn Gly Ala Pro Lys 
705                 710                 715                 720 


Thr Ile Ile Glu Leu Met Arg Asn Asp Ser Tyr Asn Leu Met Glu Ile 
                725                 730                 735     


Leu Gly Asp Lys Phe Ser Phe Met Glu Cys Ile Glu Glu Glu Asn Ala 
            740                 745                 750         


Lys Leu Ala Gln Gly Gln Val Val Asn Pro His Asp Ile Ile Asp Glu 
        755                 760                 765             


Leu Ala Leu Ser Pro Ala Val Lys Arg Ala Val Trp Gln Ala Leu Arg 
    770                 775                 780                 


Ile Val Asp Glu Val Ala His Ile Lys Lys Ala Leu Pro Ser Arg Ile 
785                 790                 795                 800 


Phe Val Glu Val Ala Arg Thr Asn Lys Ser Glu Lys Lys Lys Lys Asp 
                805                 810                 815     


Ser Arg Gln Lys Arg Leu Ser Asp Leu Tyr Ser Ala Ile Lys Lys Asp 
            820                 825                 830         


Asp Val Leu Gln Ser Gly Leu Gln Asp Lys Glu Phe Gly Ala Leu Lys 
        835                 840                 845             


Ser Gly Leu Ala Asn Tyr Asp Asp Ala Ala Leu Arg Ser Lys Lys Leu 
    850                 855                 860                 


Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Cys Ala Tyr Thr Gly Asn Ile 
865                 870                 875                 880 


Ile Asp Leu Asn Gln Leu Asn Thr Asp Asn Tyr Asp Ile Asp His Ile 
                885                 890                 895     


Tyr Pro Arg Ser Leu Thr Lys Asp Asp Ser Phe Asp Asn Leu Val Leu 
            900                 905                 910         


Cys Glu Arg Thr Ala Asn Ala Lys Lys Ser Asp Ile Tyr Pro Ile Asp 
        915                 920                 925             


Asn Arg Ile Gln Thr Lys Gln Lys Pro Phe Trp Ala Phe Leu Lys His 
    930                 935                 940                 


Gln Gly Leu Ile Ser Glu Arg Lys Tyr Glu Arg Leu Thr Arg Ile Ala 
945                 950                 955                 960 


Pro Leu Thr Ala Asp Asp Leu Ser Gly Phe Ile Ala Arg Gln Leu Val 
                965                 970                 975     


Glu Thr Asn Gln Ser Val Lys Ala Thr Thr Thr Leu Leu Arg Arg Leu 
            980                 985                 990         


Tyr Pro Asp Ile Asp Val Val Phe  Val Lys Ala Glu Asn  Val Ser Asp 
        995                 1000                 1005             


Phe Arg  His Asn Asn Asn Phe  Ile Lys Val Arg Ser  Leu Asn His 
    1010                 1015                 1020             


His His  His Ala Lys Asp Ala  Tyr Leu Asn Ile Val  Val Gly Asn 
    1025                 1030                 1035             


Val Tyr  His Glu Lys Phe Thr  Arg Asn Phe Arg Leu  Phe Phe Lys 
    1040                 1045                 1050             


Lys Asn  Gly Ala Asn Arg Thr  Tyr Asn Leu Ala Lys  Met Phe Asn 
    1055                 1060                 1065             


Tyr Asp  Val Ile Cys Thr Asn  Ala Gln Asp Gly Lys  Ala Trp Asp 
    1070                 1075                 1080             


Val Lys  Thr Ser Met Asn Thr  Val Lys Lys Met Met  Ala Ser Asn 
    1085                 1090                 1095             


Asp Val  Arg Val Thr Arg Arg  Leu Leu Glu Gln Ser  Gly Ala Leu 
    1100                 1105                 1110             


Ala Asp  Ala Thr Ile Tyr Lys  Ala Ser Val Ala Ala  Lys Ala Lys 
    1115                 1120                 1125             


Asp Gly  Ala Tyr Ile Gly Met  Lys Thr Lys Tyr Ser  Val Phe Ala 
    1130                 1135                 1140             


Asp Val  Thr Lys Tyr Gly Gly  Met Thr Lys Ile Lys  Asn Ala Tyr 
    1145                 1150                 1155             


Ser Ile  Ile Val Gln Tyr Thr  Gly Lys Lys Gly Glu  Glu Ile Lys 
    1160                 1165                 1170             


Glu Ile  Val Pro Leu Pro Ile  Tyr Leu Ile Asn Arg  Asn Ala Thr 
    1175                 1180                 1185             


Asp Ile  Glu Leu Ile Asp Tyr  Val Lys Ser Val Ile  Pro Lys Ala 
    1190                 1195                 1200             


Lys Asp  Ile Ser Ile Lys Tyr  Arg Lys Leu Cys Ile  Asn Gln Leu 
    1205                 1210                 1215             


Val Lys  Val Asn Gly Phe Tyr  Tyr Tyr Leu Gly Gly  Lys Thr Asn 
    1220                 1225                 1230             


Asp Lys  Ile Tyr Ile Asp Asn  Ala Ile Glu Leu Val  Val Pro His 
    1235                 1240                 1245             


Asp Ile  Ala Thr Tyr Ile Lys  Leu Leu Asp Lys Tyr  Asp Leu Leu 
    1250                 1255                 1260             


Arg Lys  Glu Asn Lys Thr Leu  Lys Ala Ser Ser Ile  Thr Thr Ser 
    1265                 1270                 1275             


Ile Tyr  Asn Ile Asn Thr Ser  Thr Val Val Ser Leu  Ser Asn Lys 
    1280                 1285                 1290             


Val Gly  Ile Asp Val Phe Asp  Tyr Phe Met Ser Lys  Leu Arg Thr 
    1295                 1300                 1305             


Pro Leu  Tyr Met Lys Met Lys  Gly Asn Lys Val Asp  Glu Leu Ser 
    1310                 1315                 1320             


Ser Thr  Gly Arg Ser Lys Phe  Ile Lys Met Thr Leu  Glu Glu Gln 
    1325                 1330                 1335             


Ser Ile  Tyr Leu Leu Glu Val  Leu Asn Leu Leu Thr  Asn Ser Lys 
    1340                 1345                 1350             


Thr Thr  Phe Asp Val Lys Pro  Leu Gly Ile Thr Gly  Ser Arg Ser 
    1355                 1360                 1365             


Thr Ile  Gly Val Lys Ile His  Asn Leu Asp Glu Phe  Lys Ile Ile 
    1370                 1375                 1380             


Asn Glu  Ser Ile Thr Gly Leu  Tyr Ser Asn Glu Val  Thr Ile Val 
    1385                 1390                 1395             


<210>  1174
<211>  1399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1174

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Glu Ala Glu Pro Phe Ser Asp Glu Lys Ser Glu Ile 
            20                  25                  30          


Val Arg Leu Gly Val Arg Val Ile Pro Leu Thr Val Asp Glu Arg Thr 
        35                  40                  45              


Ser Phe Glu Asn Gly Lys Ser Ile Thr Thr Asn Ala Leu Arg Thr Gln 
    50                  55                  60                  


Lys Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Gln 
65                  70                  75                  80  


Ala Leu Ile Glu Glu Leu Lys Ser Thr Gly Trp Ile Gly Asn Asp Phe 
                85                  90                  95      


Ile Phe Ser Glu Thr Gly Pro Gly Ser Thr Phe Arg Thr Leu Arg Leu 
            100                 105                 110         


Arg Ala Lys Ser Ala Ser Glu Glu Val Thr Leu Gly Asp Leu Ala Arg 
        115                 120                 125             


Ile Leu Leu Gln Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys 
    130                 135                 140                 


Met Val Ser Ser Asp Asp Gly Gln Ala Ile Asp Gly Met Ala Val Ala 
145                 150                 155                 160 


Lys Glu Met Tyr Asp Lys Gly Leu Thr Pro Gly Glu Tyr Val Tyr Gln 
                165                 170                 175     


Arg Met Leu Glu Gly Lys Tyr Ala Ile Pro Glu Phe Tyr Lys Ser Asp 
            180                 185                 190         


Leu Gln Glu Glu Phe Asp Arg Ile Trp Ala Leu Gln Lys Ser Phe His 
        195                 200                 205             


Ile Glu Leu Thr Asp Glu Leu Tyr Arg Lys Leu Ser Asp Arg Asn Lys 
    210                 215                 220                 


Ser Gln Thr Trp Ala Ile Cys Arg Asp Glu Leu Gly Ile Gln Gly Leu 
225                 230                 235                 240 


Lys Ser Ser Phe Ile Gly Lys Glu Arg Leu Lys Glu Ala Tyr Arg Tyr 
                245                 250                 255     


Arg His Leu Ala Ala Thr Gln Glu Val Asn Met Glu Val Leu Thr Cys 
            260                 265                 270         


Ala Leu Gln Glu Ile Asn Gly Gln Lys Ala Ser Leu Ser Gly Leu Leu 
        275                 280                 285             


Ser Lys Ile Ser Asp Arg Ser Lys Glu Leu Tyr Leu Asn Asn Leu Thr 
    290                 295                 300                 


Val Gly Gln Met Leu Leu Lys Arg Ile Glu Asp Asn Pro Asn Asn Ser 
305                 310                 315                 320 


Leu Lys Asn Ile Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Arg Ile Trp Thr Val Gln Ser Lys Tyr His Thr Glu Leu Thr His Glu 
            340                 345                 350         


Leu Lys Lys Arg Leu Arg Asp Ile Ile Ile Phe Tyr Gln Arg Pro Leu 
        355                 360                 365             


Lys Ser Gln Lys Gly Leu Ile Ser Leu Cys Glu Leu Glu Thr Lys Asp 
    370                 375                 380                 


Ile Glu Val Ala Ile Asp Gly Val Lys Lys Met Lys Asn Ile Gly Pro 
385                 390                 395                 400 


Arg Val Cys Pro Arg Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp 
                405                 410                 415     


Gln Thr Ile Asn Asn Ile Phe Val Asn Gly Ala Ala Leu Asp Asp Glu 
            420                 425                 430         


Asp Lys Ser Val Ile Ala Lys Glu Leu Met Phe Lys Asp Lys Leu Lys 
        435                 440                 445             


Asp Ser Asp Leu Leu Lys Leu Leu Phe Lys Thr Thr Lys Gly Gln Ser 
    450                 455                 460                 


Val Asn Phe Lys Glu Val Asp Gly Asn Arg Thr Met Ala Thr Leu Met 
465                 470                 475                 480 


Lys Ala Cys Phe Lys Ile Ala Glu Asn Leu Gly Tyr Asp Asp Ile Gly 
                485                 490                 495     


Leu Ser Lys Leu Ser Ser Glu Asp Gln Met Val Arg Ile Glu Gly Ile 
            500                 505                 510         


Phe Lys Glu Gln Gly Phe Lys Thr Gly Phe Leu His Phe Asp Ser Ser 
        515                 520                 525             


Leu Ala Ser Pro Glu Phe Glu Asn Gln Asp Ser Tyr Arg Leu Trp His 
    530                 535                 540                 


Leu Leu Tyr Ser Tyr Glu Gly Asp Lys Ser Ala Ser Gly Asn Glu Ser 
545                 550                 555                 560 


Leu Val Lys Cys Ile Met Asp Leu Cys Ser Met Asp Glu Gly Ser Ala 
                565                 570                 575     


Lys Ile Leu Ala Ser Cys Thr Phe Leu Pro Asp Tyr Gly Ser Leu Ser 
            580                 585                 590         


Ala Lys Ala Met Arg Lys Ile Leu Pro Tyr Met Lys Asp Gly Leu Glu 
        595                 600                 605             


Tyr Thr Ala Ala Cys Ala Met Ala Gly Tyr Arg His Ser Lys Arg Ser 
    610                 615                 620                 


Leu Thr Lys Glu Glu Leu Asp Thr Lys Glu Tyr Ser Asp Arg Leu Ser 
625                 630                 635                 640 


Leu Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu 
                645                 650                 655     


Asn Gln Met Ile Asn Val Val Asn Ser Val Ile Asp Val Tyr Gly Lys 
            660                 665                 670         


Pro Asp Glu Ile Arg Ile Glu Met Ala Arg Glu Leu Lys Lys Ser Ala 
        675                 680                 685             


Lys Glu Arg Glu Glu Ala Gln Arg Asp Ile Ala Ser Ser Thr Ala Glu 
    690                 695                 700                 


Ser Gln Lys Val Ile Glu Ile Leu Lys Gly Glu Pro Phe Phe Leu Gln 
705                 710                 715                 720 


Phe Pro Thr Arg Asn Asp Ile Ile Arg Tyr Arg Leu Tyr Gln Glu Leu 
                725                 730                 735     


Ala Gln Asn Gly Phe Lys Thr Leu Tyr Ser Asn Thr Tyr Ile Pro Lys 
            740                 745                 750         


Asp Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile Pro Gln 
        755                 760                 765             


Ala Leu Ile Phe Asn Asp Ser Phe Ala Asn Lys Thr Leu Glu Ser Arg 
    770                 775                 780                 


Gln Cys Asn Leu Glu Lys Gly Lys Glu Thr Ala Met Asp Tyr Val Lys 
785                 790                 795                 800 


Ser Lys Tyr Asp Glu Thr Gly Val Gln Thr Tyr Cys Asp Arg Val Asn 
                805                 810                 815     


Gly Leu Ile Gly Lys Gly Ile Ser Lys Thr Lys Ala Lys Trp Leu Leu 
            820                 825                 830         


Met Glu Gly Lys Asp Ile Pro Glu Asp Phe Ile Asn Arg Glu Leu Arg 
        835                 840                 845             


Asp Ser Gln Tyr Ile Ala Lys Lys Ala Arg Glu Ile Leu Glu Ser Leu 
    850                 855                 860                 


Val Lys Phe Val Val Pro Thr Thr Gly Ser Val Thr Ala Arg Leu Arg 
865                 870                 875                 880 


Glu Asp Trp Gln Leu Val Asn Val Met Gln Glu Leu Asn Trp Glu Lys 
                885                 890                 895     


Tyr Ala Arg Leu Gly Leu Thr Gly Val Arg Glu Asp Lys Asp Gly Arg 
            900                 905                 910         


Arg Ile Pro Phe Ile Gln Asp Trp Thr Lys Arg Asn Asp His Arg His 
        915                 920                 925             


His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr Arg Arg Gln Phe Ile 
    930                 935                 940                 


Gln Tyr Leu Asn His Leu Asn Ser Lys Ile Asp Val Val Ser Trp Asp 
945                 950                 955                 960 


Lys Lys Asp Leu Asp Leu Arg Asp Tyr Asp Leu Glu Asp Ile Lys Phe 
                965                 970                 975     


Gly Asn Leu Ser Ala Gly Asp Arg Tyr Gly Ile Val Lys Ala Leu Gln 
            980                 985                 990         


Asp Lys Phe Leu Tyr Lys Asp Gly  Asn Asp Lys Tyr Arg  Phe Val Pro 
        995                 1000                 1005             


Pro Ile  Pro Leu Asp Glu Phe  Arg Arg Gln Ala Lys  Glu Gln Leu 
    1010                 1015                 1020             


Ser Asp  Ile Leu Ile Ser Phe  Lys Ala Lys Asn Lys  Val Cys Thr 
    1025                 1030                 1035             


Arg Asn  Val Asn Val Thr Lys  Asn Lys Gly Gly Ala  Asn Arg Lys 
    1040                 1045                 1050             


Ala Gln  Leu Thr Pro Arg Gly  Pro Leu His Asn Glu  Thr Ile Tyr 
    1055                 1060                 1065             


Gly Ser  Ser Leu Glu Tyr Val  Thr Lys Glu Asn Glu  Lys Ile Gly 
    1070                 1075                 1080             


Ser Ser  Phe Asp Thr Glu Arg  Ile Thr Thr Val Cys  Lys Lys Lys 
    1085                 1090                 1095             


Phe Arg  Asp Ala Leu Ala Arg  Arg Leu Glu Glu Phe  Gly Gly Asp 
    1100                 1105                 1110             


Pro Lys  Lys Ala Phe Thr Gly  Lys Asn Ser Pro Glu  Lys Asn Pro 
    1115                 1120                 1125             


Ile Trp  Val Asp Glu His His  Ser Glu Gln Val Pro  Ala Lys Val 
    1130                 1135                 1140             


Arg Thr  Val Thr Met Gly Gln  Arg Phe Thr Gly Arg  Lys Pro Ile 
    1145                 1150                 1155             


Asp Ala  Thr Leu Lys Ile Glu  Lys Val Ile Asp Lys  Arg Ile Arg 
    1160                 1165                 1170             


Glu Ile  Leu Gln Ala Arg Leu  Asp Glu Phe Asp Gly  Lys Ala Ala 
    1175                 1180                 1185             


Lys Ala  Phe Ser Asn Leu Asp  Glu Asn Pro Ile Trp  Leu Asn Lys 
    1190                 1195                 1200             


Glu Lys  Gly Ile Ala Ile Lys  Arg Val Thr Val Ser  Gly Pro Ala 
    1205                 1210                 1215             


Asn Pro  Val Pro Val Arg Phe  Lys Arg Asp Lys Asp  Gly Lys Pro 
    1220                 1225                 1230             


Ile Ile  Asp Asp Ala Gly Lys  Thr Ile Gly Ala Asp  Phe Val Thr 
    1235                 1240                 1245             


Pro Gly  Asn Asn His His Ile  Ala Ile Phe Arg Asp  Ser Ser Gly 
    1250                 1255                 1260             


Lys Leu  Gln Glu His Pro Val  Ser Phe Leu Glu Ala  Thr Ile Ala 
    1265                 1270                 1275             


Lys Ser  His Gly Leu Asp Val  Ile Asp Arg Asn Tyr  Asn Lys Asp 
    1280                 1285                 1290             


Glu Gly  Trp Glu Phe Leu Phe  Thr Leu Lys Gln Asn  Glu Tyr Phe 
    1295                 1300                 1305             


Val Phe  Pro Asn Ser Glu Thr  Gly Phe Asn Pro Leu  Asp Tyr Asp 
    1310                 1315                 1320             


Leu Thr  Asp Pro Arg Asn Tyr  Ala Glu Ile Ser Pro  Asn Leu Tyr 
    1325                 1330                 1335             


Arg Val  Gln Ser Ile Ser Thr  Asn Asp Tyr Phe Phe  Arg His His 
    1340                 1345                 1350             


Leu Glu  Thr Thr Ser Glu Lys  Asn Asn Ser Leu Tyr  Gly Ile Thr 
    1355                 1360                 1365             


Trp Lys  Arg Ile Arg Asn Ala  Ser Ala Leu Glu Gly  Leu Val Lys 
    1370                 1375                 1380             


Val Arg  Ile Asp Asn Leu Gly  Arg Ile Val Ala Val  Gly Glu Tyr 
    1385                 1390                 1395             


Asp 
    


<210>  1175
<211>  1399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1175

Met Glu Thr Gln Ala Thr Ala Gln Leu Ile Ser Gly His Leu Lys Gly 
1               5                   10                  15      


Tyr Pro Ile Tyr Asp Tyr Phe Val Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Val Thr Asn Arg Ile Tyr Glu Leu Leu Lys Phe Arg 
        35                  40                  45              


Ser His Lys Met Trp Gly Ser Arg Leu Phe Glu Glu Gly Gln Thr Ala 
    50                  55                  60                  


Val Val Arg Arg Gly Phe Arg Ser Ile Arg Arg Arg Leu Glu Arg Arg 
65                  70                  75                  80  


Arg Leu Arg Leu Lys Leu Leu Glu Ala Leu Phe Ala Asp Ala Met Ala 
                85                  90                  95      


Arg Val Asp Ser Thr Phe Phe Ile Arg Leu Arg Glu Ser Lys Tyr His 
            100                 105                 110         


Tyr Glu Asp Lys Thr Thr Gly His Lys Ser Lys His Ile Leu Phe Ile 
        115                 120                 125             


Asp Asp Asn Tyr Thr Asp Gln Asp Tyr Phe Ala Glu Tyr Pro Thr Ile 
    130                 135                 140                 


Tyr His Leu Arg Ala Asp Leu Met Glu Asn Gly Thr Ser Asp Ile Arg 
145                 150                 155                 160 


Lys Leu Phe Leu Ala Val His His Ile Leu Lys Tyr Arg Gly Asn Phe 
                165                 170                 175     


Leu Tyr Glu Gly Ala Thr Phe Asp Ser Asn Ser Ser Leu Asp Thr Val 
            180                 185                 190         


Leu Lys Arg Ala Leu Glu Gln Ile Glu Phe His Ile Phe Asn Ala Thr 
        195                 200                 205             


Ser Glu Asp Ala Leu His Ser Ile Leu Asp Ile Val Lys Leu Gln Asp 
    210                 215                 220                 


Lys Thr Lys Ser Asp Lys Ala Lys Ala Ile Glu Ser Leu Val Asp Thr 
225                 230                 235                 240 


Tyr Ile Thr Asp Lys Val Glu Ser Ser Cys Thr Asp Lys Glu Lys Lys 
                245                 250                 255     


Asp Leu Leu Lys Ala Asp Lys Lys Arg Leu Arg Ala Phe Ala Asn Leu 
            260                 265                 270         


Ile Leu Gly Leu Asn Ala Asn Leu Thr Asp Leu Phe Gly Thr Ile Glu 
        275                 280                 285             


Glu Ile Glu Glu Asp Phe Val Lys Leu Gln Ile Thr Gly Asp Thr Tyr 
    290                 295                 300                 


Glu Glu Lys Arg Asp Asp Tyr Ala Lys Val Trp Gly Asp Thr Ile Arg 
305                 310                 315                 320 


Ile Ile Asp Asp Cys Lys Ser Val Tyr Asp Ser Ile Ile Leu Met Ser 
                325                 330                 335     


Ile Lys Glu Pro Gly Leu Thr Ile Ser Gln Ser Lys Val Lys Ala Phe 
            340                 345                 350         


Asn Lys His Lys Glu Asp Leu Ala Ile Leu Lys Arg Leu Leu Lys Ser 
        355                 360                 365             


Asp Lys Tyr Ile Tyr Lys Asn Met Phe Lys Ala Asp Glu Lys Gly Leu 
    370                 375                 380                 


Ala Asn Tyr Val His Tyr Ile Lys Gln Gly Arg Lys Asn Glu Thr Gly 
385                 390                 395                 400 


Cys Ser Cys Glu Asp Phe Tyr Lys Tyr Thr Lys Lys Val Val Glu Ser 
                405                 410                 415     


Leu Pro Asp Ser Ser Asp Lys Ala Tyr Ile Leu Asn Glu Ile Glu Leu 
            420                 425                 430         


Gln Lys Leu Leu Pro Leu Gln Arg Ile Lys Asp Asn Gly Val Ile Pro 
        435                 440                 445             


Tyr Gln Leu His Leu Glu Glu Leu Lys Ala Ile Leu Glu Lys Cys Lys 
    450                 455                 460                 


Asp Thr Phe Pro Phe Leu Asn Thr Val Ser Asp Gly Phe Thr Val Ala 
465                 470                 475                 480 


Glu Lys Leu Ile Lys Met Leu Glu Phe Arg Ile Pro Tyr Tyr Val Gly 
                485                 490                 495     


Pro Leu Asn Pro Ser His Ser Ile Asp Lys Gly Gly Phe Ala Trp Val 
            500                 505                 510         


Glu Arg Lys Glu Pro Gly Gln Val Thr Pro Trp Asn Phe Glu Asp Lys 
        515                 520                 525             


Ile Asp Arg Glu Lys Ser Ala Glu Ala Phe Ile Lys Asn Leu Thr Asn 
    530                 535                 540                 


Lys Cys Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Ser Ser Leu 
545                 550                 555                 560 


Leu Tyr Ser Glu Phe Met Leu Leu Asn Glu Leu Asn Asn Leu Arg Ile 
                565                 570                 575     


Asp Gly Lys Pro Leu Glu Gln Ala Val Lys Met His Leu Ile Asp Ala 
            580                 585                 590         


Val Phe Lys Lys Glu His Lys Lys Gln Thr Lys Asn Arg Ile Ile Glu 
        595                 600                 605             


Phe Leu Lys Asp Asn Asn Tyr Ile Leu Gly Lys His Lys Pro Glu Val 
    610                 615                 620                 


Asp Gly Leu Asp Glu Ser Ile Lys Gly Asp Leu Ala Ser Tyr Arg Asp 
625                 630                 635                 640 


Met Lys Arg Ile Leu Gly Asp Asp Phe Asp Val Ser Met Ala Glu Ser 
                645                 650                 655     


Ile Ile Thr Ser Ile Thr Ile Phe Gly Glu Ser Lys Asn Met Leu Arg 
            660                 665                 670         


Glu Thr Leu Arg Lys Lys Phe Gly Ser Ile Leu Ser Glu Asp Gln Ile 
        675                 680                 685             


Arg Ser Leu Ala Lys Leu Arg Tyr Arg Asp Trp Gly Arg Leu Ser Lys 
    690                 695                 700                 


Lys Phe Leu Ile Gly Ile Asp Gly Cys Val Lys Gly Glu Asp Gly Thr 
705                 710                 715                 720 


Pro Gly Thr Ile Ile Asp Met Met Arg Asn Thr Ser His Asn Leu Met 
                725                 730                 735     


Glu Leu Leu Gly Asp Thr Phe Ser Phe Met Asp Val Ile Ile Glu Glu 
            740                 745                 750         


Asn Asp Lys Leu Thr Lys Gly Gln Val Val Asp Pro His Glu Ile Ile 
        755                 760                 765             


Glu Glu Leu Gly Leu Ser Pro Ala Val Lys Arg Ala Val Trp Gln Ala 
    770                 775                 780                 


Leu Arg Ile Val Asp Glu Val Ile His Ile Lys Lys Ala Leu Pro Ser 
785                 790                 795                 800 


Arg Ile Phe Val Glu Val Ala Arg Thr Asn Lys Asp Glu Lys Lys Lys 
                805                 810                 815     


Lys Asp Ser Arg Lys Lys Arg Leu Gln Glu Leu Tyr Lys Ser Ile Gln 
            820                 825                 830         


Lys Glu Ser Pro Glu Trp Leu Ser Val Thr Arg Asp Glu Ser Asp Lys 
        835                 840                 845             


Leu Ile Ser Asn Leu Asp Arg Ala Asp Asp Ser Ser Leu Arg Ser Lys 
    850                 855                 860                 


Lys Leu Tyr Leu Tyr Tyr Thr Gln Met Gly Arg Cys Met Tyr Ser Gly 
865                 870                 875                 880 


Arg Val Ile Asp Leu Asp Gln Leu Asn Thr Asp Lys Tyr Asp Ile Asp 
                885                 890                 895     


His Ile Tyr Pro Arg Ser Leu Thr Lys Asp Asp Ser Phe Asp Asn Leu 
            900                 905                 910         


Val Leu Cys Glu Arg Gln Glu Asn Ala Lys Lys Ser Asp Asn Tyr Pro 
        915                 920                 925             


Ile Asp Pro Asp Ile Gln Asn Ala Arg Lys Pro Phe Trp Thr Phe Leu 
    930                 935                 940                 


Lys Asp Gln Asn Leu Ile Ser Ser Arg Lys Tyr Glu Arg Leu Thr Arg 
945                 950                 955                 960 


Val Leu Pro Leu Glu Ala Asp Asp Leu Thr Gly Phe Ile Ala Arg Gln 
                965                 970                 975     


Leu Val Glu Thr Asn Gln Ser Val Lys Ala Val Thr Thr Leu Leu Arg 
            980                 985                 990         


Arg Leu Tyr Pro Gly Val Asp Val  Val Phe Val Lys Ala  Glu Asn Val 
        995                 1000                 1005             


Thr Asp  Phe Arg His Asp Asn  Asp Phe Ile Lys Val  Arg Ser Leu 
    1010                 1015                 1020             


Asn His  His His His Ala Lys  Asp Ala Tyr Leu Asn  Ile Val Val 
    1025                 1030                 1035             


Gly Asn  Val Tyr His Glu Lys  Phe Thr Arg Asn Phe  Arg Lys Phe 
    1040                 1045                 1050             


Leu Lys  Lys Asn Gly Ala Lys  Arg Thr Tyr Asn Leu  Ala Arg Met 
    1055                 1060                 1065             


Phe Asp  Phe Asp Val Met Ser  Thr Asn Ala Lys Asp  Gly Lys Ala 
    1070                 1075                 1080             


Trp Asp  Ile Lys Thr Ser Met  Asn Ile Val Lys Lys  Met Met Ala 
    1085                 1090                 1095             


Ser Asn  Asp Val Arg Val Thr  Lys Arg Leu Met Glu  Gln Lys Gly 
    1100                 1105                 1110             


Ala Leu  Ser Asp Ala Thr Val  Tyr Lys Ala Ser Val  Ala Gly Lys 
    1115                 1120                 1125             


Ala Lys  Gly Gly Ala Tyr Ile  Gly Met Lys Thr Lys  Asp Ser Val 
    1130                 1135                 1140             


Phe Ser  Asp Val Ser Lys Tyr  Gly Gly Arg Thr Ser  Ile Lys Asn 
    1145                 1150                 1155             


Ala Tyr  Ser Ile Val Val Glu  Tyr Thr Gly Lys Lys  Asn Glu Ile 
    1160                 1165                 1170             


Ile Lys  Glu Ile Val Pro Leu  Pro Val Tyr Leu Ile  Asn Arg Asn 
    1175                 1180                 1185             


Lys Thr  Asp Gln Glu Leu Ile  His Tyr Ile Val Ser  Ile Ile Pro 
    1190                 1195                 1200             


Gln Ala  Lys Asn Val Ser Ile  Ile Tyr Lys Lys Leu  Cys Ile Asn 
    1205                 1210                 1215             


Gln Leu  Val Lys Val Asn Gly  Phe Tyr Tyr Tyr Leu  Gly Gly Lys 
    1220                 1225                 1230             


Thr Asn  Asn Tyr Phe Trp Ile  Asp Ser Ala Ile Gln  Val Val Ile 
    1235                 1240                 1245             


Ala Asp  Glu Trp Ile Pro Tyr  Leu Lys Val Leu Glu  Lys Phe Asp 
    1250                 1255                 1260             


Val Met  Arg Lys Gln Asn Lys  Glu Leu Lys Val Gly  Thr Val Ser 
    1265                 1270                 1275             


Thr Ser  Ile Gln Asn Gln Gly  Lys Lys Val Val Ile  Lys Ile Leu 
    1280                 1285                 1290             


Glu Glu  Arg Asn Val Glu Phe  Phe Ser Tyr Leu Val  Gly Lys Leu 
    1295                 1300                 1305             


His Ser  Ser Ile Tyr Ser Lys  Met Lys Gly Asn Lys  Ala Gly Glu 
    1310                 1315                 1320             


Leu Ala  Thr Lys Ser Leu Glu  Ile Phe Val Asn Met  Ser Leu Glu 
    1325                 1330                 1335             


Asn Gln  Cys Ile Tyr Leu Met  Glu Leu Leu Asn Leu  Leu Thr Asn 
    1340                 1345                 1350             


Lys Lys  Ala Pro Asp Val Lys  Pro Leu Gly Ile Thr  Ala Ser Arg 
    1355                 1360                 1365             


Gly Thr  Ile Gly Ser Lys Val  Ser Asn Gln Asp Glu  Phe Lys Ile 
    1370                 1375                 1380             


Ile Asn  Glu Ser Ile Thr Gly  Leu Tyr Ser Asn Glu  Val Thr Ile 
    1385                 1390                 1395             


Val 
    


<210>  1176
<211>  1399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1176

Met Asn Leu Arg Asn Thr Thr Asp Glu Tyr Asn Ile Gly Leu Asp Met 
1               5                   10                  15      


Gly Thr Asn Ser Val Gly Trp Ala Val Thr Asp Lys Ala Gly Ala Ile 
            20                  25                  30          


Ala His Phe Lys Gly Lys Pro Thr Leu Gly Ser Arg Leu Phe Asp Ala 
        35                  40                  45              


Ala Gln Thr Ala Ala Gly Ala Arg Ile His Arg Ser Gln Arg Arg Arg 
    50                  55                  60                  


Tyr Val Arg Arg Arg Trp Arg Leu Asp Leu Leu Gln Ser Leu Phe Gln 
65                  70                  75                  80  


Asp Glu Val Ser Lys Val Asp Ser Glu Phe Phe Cys Arg Leu Arg Gln 
                85                  90                  95      


Ser Arg Leu Tyr Val Gly Asp Arg Glu Lys Gly His Ala Asp Tyr Thr 
            100                 105                 110         


Trp Pro Ile Phe Asn Asp Ser Asp Phe Asn Glu Val Asp Tyr Tyr Lys 
        115                 120                 125             


Arg Phe Pro Thr Ile Tyr His Leu Arg Lys Trp Leu Met Glu Thr Asp 
    130                 135                 140                 


Glu Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Thr His Asn Ile Val 
145                 150                 155                 160 


Lys His Arg Gly Asn Phe Leu Arg Glu Asn Asn Lys Ser Leu Ser Ser 
                165                 170                 175     


Arg Asp Ala Asn Pro Ala Gln Ala Val Lys Ser Leu Arg Leu Ala Leu 
            180                 185                 190         


Asn Asp Trp Cys Ser Glu Arg Asp Tyr Glu Cys Ala Trp Lys Gly Glu 
        195                 200                 205             


Glu Asp Asp Gly Ala Ile Asp Glu Ala Ile Leu Asn Val Leu Ala Asp 
    210                 215                 220                 


Gly Glu Lys Gly Arg Ser Asp Gln Ala Lys Glu Ile Ala Lys Leu Thr 
225                 230                 235                 240 


Gly Val Asp Cys Gly Asp Ala Lys Gln Ser Lys Gln Cys Asn Glu Ala 
                245                 250                 255     


Ile Ala Lys Ala Ile Val Gly Leu Lys Val Glu Met Lys Asn Ile Phe 
            260                 265                 270         


Gly Glu Phe Pro Ala Glu Lys Thr Ser Tyr Ile Tyr Leu Ser Ser Asp 
        275                 280                 285             


Glu Asp Val Glu Ala Val Arg Glu Ser Cys Pro Asp Asp Ser Gly Glu 
    290                 295                 300                 


Leu Phe Gln Ala Leu Cys Lys Leu Tyr Ser Ala Tyr Ile Leu Gln Glu 
305                 310                 315                 320 


Leu Leu Ser Tyr Ala Asp Pro Ser Gly Gln Thr Ile Ser Ala Asn Met 
                325                 330                 335     


Val Ala Lys Tyr Lys Gln Tyr Gly Arg Asp Leu Arg Leu Leu Lys Glu 
            340                 345                 350         


Leu Thr Arg Thr Tyr Ala Pro Gln Lys Tyr Asp Leu Phe Phe Arg Gly 
        355                 360                 365             


Glu Thr Tyr Ala Gly Ser Ser Asp Tyr Val Val Asp Glu Ala Arg Gly 
    370                 375                 380                 


Tyr Thr Arg Tyr Asn Leu Gly Thr Thr Lys Ala Asn Tyr Asp Asp Phe 
385                 390                 395                 400 


Gln Lys Ser Val Lys Glu Leu Phe Lys Gly Thr Asp Ala Leu Gln Asp 
                405                 410                 415     


Glu Arg Tyr Gln Glu Met Met Val Ala Phe Gly Glu Gln Lys Phe Leu 
            420                 425                 430         


Arg Arg Leu Lys Thr Ser Asp Asn Gly Ser Ile Tyr Tyr Gln Leu His 
        435                 440                 445             


Leu Glu Glu Leu His Thr Ile Leu Glu Asn Gln Gly Lys His Tyr Pro 
    450                 455                 460                 


Phe Leu Leu Ser Glu Glu Asp Lys Ile Glu Ser Leu Val Ser Phe Arg 
465                 470                 475                 480 


Ile Pro Tyr Tyr Val Gly Pro Leu Thr Arg Lys Asn Ala Ala Ile Gly 
                485                 490                 495     


His Asp Gly Lys Glu Arg Phe Ala Trp Ser Ser Arg Lys Asp Gly Met 
            500                 505                 510         


Glu His Ala Val Ile Thr Pro Trp Asn Trp Glu Glu Ile Ile Asp Arg 
        515                 520                 525             


Asp Lys Ser Ala Glu Asp Phe Ile Met Arg Met Thr Gly Asp Cys Thr 
    530                 535                 540                 


Tyr Leu Gln Gly Glu Pro Val Leu Pro Lys Cys Ser Leu Leu Tyr Glu 
545                 550                 555                 560 


Glu Phe Cys Ala Leu Asn Glu Phe Asn Gly Ala His Trp Thr Val Asp 
                565                 570                 575     


Gly Asp Asp Glu Arg Arg Phe Asp Ala Ala Gln Arg Glu Arg Ile Met 
            580                 585                 590         


Cys Asp Leu Phe Arg Arg Thr Arg Ser Val Thr Tyr Asn Lys Val Ser 
        595                 600                 605             


Asp Trp Leu Glu Arg Lys Tyr Asn Phe Thr Gly Ala Arg Val Arg Gly 
    610                 615                 620                 


Gly Gln Gly Glu His Gly Phe Glu Ser Lys Leu Gly Ser Tyr Ile Phe 
625                 630                 635                 640 


Phe Cys Lys Asp Val Phe Lys Thr Asp Gly Leu Asp Glu Ala Asp Tyr 
                645                 650                 655     


Thr Met Ile Glu Gln Ile Ile Leu Trp Ser Thr Leu Phe Glu Asp Arg 
            660                 665                 670         


Asp Ile Leu Lys Arg Lys Ile Glu Glu Ala Tyr Gly Gly Asp Arg Leu 
        675                 680                 685             


Thr Ser Glu Gln Ile Lys Val Ile Cys Lys Lys Arg Phe Thr Gly Trp 
    690                 695                 700                 


Gly Arg Leu Ser Lys Arg Leu Leu Thr Asp Ile Lys Val Asn Thr Asp 
705                 710                 715                 720 


Thr Gly Arg Lys Ser Ile Met Asp Val Leu Arg Glu Gly Asn Pro Asn 
                725                 730                 735     


Asn Gly Gln Arg Ser Arg Thr Met Val Phe Met Glu Val Leu Arg Asp 
            740                 745                 750         


Glu Asp Leu Gly Phe Gln Lys Ala Ile Asp Gly Leu Asn Arg Asp Tyr 
        755                 760                 765             


Phe Arg Glu His Asn Asn Gly Leu Met Asp Val Asn Asp Leu Pro Gly 
    770                 775                 780                 


Ser Pro Ala Ile Arg Arg Ser Ile Asn Gln Ala Val Arg Ile Val Asp 
785                 790                 795                 800 


Glu Ile Ala Ser Ile Ala Arg Lys Glu Pro Thr Asn Ile Phe Ile Glu 
                805                 810                 815     


Val Thr Arg Glu Glu Asp Asp Pro Arg Lys Lys Gly Lys Arg Thr Lys 
            820                 825                 830         


Arg Arg Tyr Asp Ala Ile Lys Ser Ala Leu Glu Lys Phe Lys Lys Asp 
        835                 840                 845             


Asp Pro Ser Leu Trp Lys Glu Leu Cys Glu Lys Ala Pro Gly Asp Met 
    850                 855                 860                 


Asp Glu Arg Leu Ser Leu Tyr Phe Met Gln Arg Gly Lys Cys Met Tyr 
865                 870                 875                 880 


Ser Gly Arg Pro Ile Lys Ile Glu Glu Leu His Thr Gly Lys Tyr Glu 
                885                 890                 895     


Val Asp His Ile Ile Pro Arg Ser Tyr Ile Lys Asp Asp Ser Phe Glu 
            900                 905                 910         


Asn Lys Val Leu Val Tyr Arg Glu Glu Asn Gln His Lys Thr Asp Ala 
        915                 920                 925             


Leu Leu Ile Asp Ser Ser Ile Arg Gln Lys Met Gly Gly Tyr Trp Arg 
    930                 935                 940                 


Met Leu His Asp Ala Lys Leu Val Gly Asp Lys Lys Phe Arg Asn Leu 
945                 950                 955                 960 


Phe Arg Asn His Ile Asp Asp Lys Ala Met Lys Gly Phe Val Ala Arg 
                965                 970                 975     


Gln Ile Val Glu Thr Ser Gln Met Val Lys Leu Val Gln Thr Leu Phe 
            980                 985                 990         


Glu Ala Arg Tyr Pro Asn Thr Arg  Ile Val Pro Val Lys  Ala Gly Ile 
        995                 1000                 1005             


Ser His  Asp Leu Arg Glu Ala  Ala Gly Leu Val Lys  Cys Arg Glu 
    1010                 1015                 1020             


Ala Asn  Asp Phe His His Ala  His Asp Ala Phe Leu  Ala Cys Arg 
    1025                 1030                 1035             


Ile Gly  Leu Phe Ile Gln Lys  Arg His Pro Ser Val  Tyr Asp Asn 
    1040                 1045                 1050             


Pro Ile  Gly Leu Ala Arg Ile  Met Arg Asp Tyr Ala  Arg Met Gln 
    1055                 1060                 1065             


Ala Arg  Glu Tyr Lys Lys Ser  His Cys Leu Ala His  Thr Gln Gly 
    1070                 1075                 1080             


Phe Ile  Val Asn Ser Phe Leu  Ser Ser Gly Phe Asp  Glu Glu Thr 
    1085                 1090                 1095             


Gly Glu  Ile Phe Lys Asp Asp  Trp Asp Ala Lys Ala  Glu Val Glu 
    1100                 1105                 1110             


Gly Ile  Arg Arg Val Leu Asn  Cys Arg Gln Cys Phe  Ile Ser Arg 
    1115                 1120                 1125             


Met Pro  Val Glu Gly Asp Thr  Arg Tyr Gly Gly Arg  Tyr Trp Gly 
    1130                 1135                 1140             


Glu Thr  Ile Tyr Ser Pro Lys  Gly Lys His Lys His  Glu Ile Pro 
    1145                 1150                 1155             


Leu Lys  Gln Gly Leu Asp Pro  Asn Leu Tyr Gly Gly  Tyr Ser Asp 
    1160                 1165                 1170             


Leu Leu  Tyr Ser Tyr Phe Leu  Ile Tyr Glu Ala Ser  Asp Val Ser 
    1175                 1180                 1185             


Ser Gly  Glu Arg Val Leu Arg  Phe Ile Gly Ile Pro  Thr Ser Ile 
    1190                 1195                 1200             


Ala Ala  Arg Met Ala Asn Gln  Lys Arg Leu Glu Glu  Tyr Val Ser 
    1205                 1210                 1215             


Arg Leu  Ala Leu Gln Glu Asn  Leu Glu His Val Arg  Met Val Arg 
    1220                 1225                 1230             


Lys Ile  Met Lys Lys Gln Leu  Ile Glu Val Asp Asn  Cys Arg Leu 
    1235                 1240                 1245             


Leu Val  Thr Ala Ala Asp Ala  Val Thr Asn Ala Thr  Glu Leu Ala 
    1250                 1255                 1260             


Phe Ser  Leu Asp Glu Met Lys  Ile Ile Ser Thr Cys  Ala Lys Cys 
    1265                 1270                 1275             


Ile Asn  Gly Ala Asp Pro Ser  Ala Ile Asp Lys Ile  Gly Asn Ala 
    1280                 1285                 1290             


Gln Asp  Ser Phe Phe Asp Asn  Leu Lys Arg Arg Gly  Ser Asp Arg 
    1295                 1300                 1305             


Cys Ala  Leu Leu Phe Ser Gln  Leu Lys Leu Asp Lys  Leu Ser Gln 
    1310                 1315                 1320             


Asn Phe  Tyr Met Ile Ser Ser  Asp Asp Lys Ser Lys  Ile Leu Ile 
    1325                 1330                 1335             


Ser Ile  Leu Asp Arg Ile Arg  Ala Lys Ser Asn Ser  Val Asp Leu 
    1340                 1345                 1350             


Ser Leu  Val Gly Gly Ala Lys  His Ala Ala Gln Met  Lys Phe Asp 
    1355                 1360                 1365             


Phe Asn  Ala Lys Leu Asn Asn  Pro Ala Ser Ser Phe  Val Leu Ile 
    1370                 1375                 1380             


Asp Gln  Ser Val Thr Gly Met  Phe Glu Arg Arg Thr  Arg Ile Gly 
    1385                 1390                 1395             


Leu 
    


<210>  1177
<211>  1400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1177

Met Asn Leu Arg Asn Ala Thr Gly Asp Tyr Ser Ile Gly Leu Asp Leu 
1               5                   10                  15      


Gly Thr Asn Ser Val Gly Trp Ala Val Thr Asp Ser Asn Gly Ser Met 
            20                  25                  30          


Ala His Phe Lys Gly Lys Pro Thr Leu Gly Ser Arg Leu Phe Asp Ala 
        35                  40                  45              


Ala Glu Thr Ala Ala Gly Ala Arg Gln His Arg Gly Gln Arg Arg Arg 
    50                  55                  60                  


Tyr Val Arg Arg Arg Trp Arg Leu Asp Leu Leu Gln Gly Leu Phe Gln 
65                  70                  75                  80  


Asp Glu Val Asn Lys Val Asp Ser Glu Phe Phe Ile Arg Leu Arg Gln 
                85                  90                  95      


Ser Arg Leu His Val Glu Asp Arg Ala Glu Gly His Ala Gly Tyr Arg 
            100                 105                 110         


Trp Pro Leu Phe Asn Asp Thr Asp Phe Thr Glu Lys Asp Tyr Tyr Lys 
        115                 120                 125             


Arg Phe Pro Thr Ile Tyr His Leu Arg Lys Trp Leu Met Glu Thr Asp 
    130                 135                 140                 


Glu Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Ala His Asn Ile Val 
145                 150                 155                 160 


Lys His Arg Gly Asn Phe Leu Arg Glu Asp Asn Lys Asn Leu Ser Ser 
                165                 170                 175     


His Asn Ala Asn Pro Ala Glu Ala Val Arg Ser Leu Arg Gln Ala Leu 
            180                 185                 190         


Asp Asp Trp Cys Ala Glu Arg Gly Tyr Val Cys Ala Trp Thr Ser Val 
        195                 200                 205             


Asp Asp Leu Ala Glu Ala Asp Ser His Ile Leu Asp Ile Leu Thr Asn 
    210                 215                 220                 


Ser Lys Glu Arg Arg Ala Asp Gln Ala Lys Glu Leu Ala Lys Leu Phe 
225                 230                 235                 240 


Gly Val Asp Cys Gly Asp Thr Lys Gln Ser Lys Ser Cys Asn Ser Ala 
                245                 250                 255     


Leu Ala Lys Ala Val Val Gly Leu Lys Thr Glu Leu Lys Asp Val Phe 
            260                 265                 270         


Gly Glu Phe Cys Ala Glu Lys Thr Ala Ile His Leu Ser Ser Asp Asp 
        275                 280                 285             


Asp Val Glu Ala Ile Arg Asp Ala Ile Pro Asp Asp Ser Gly Glu Leu 
    290                 295                 300                 


Phe Gln Val Leu Cys Ala Val Tyr Ser Ala Tyr Val Leu Gln Glu Leu 
305                 310                 315                 320 


Leu Ser Tyr Ala Pro Gly Gln Thr Ile Ser Ala Asn Met Val Ala Lys 
                325                 330                 335     


Tyr Asp Gln Tyr Gly Lys Asp Leu Lys Leu Leu Lys Asn Leu Ala Arg 
            340                 345                 350         


Thr Tyr Val Ser Arg Glu Asn Tyr Asp Ala Phe Phe Arg Gly Gln Thr 
        355                 360                 365             


Tyr Glu Asp Ser Asn Asp Tyr Asn Val Asp Lys Ala Gln Gly Tyr Thr 
    370                 375                 380                 


Arg Tyr Asn Leu Gly Thr Thr Lys Ala Ser Tyr Asp Asp Phe Lys Lys 
385                 390                 395                 400 


Ser Val Glu Lys Leu Phe Lys Gly Thr Gln Ala Glu Gln Asp Lys Arg 
                405                 410                 415     


Tyr Gln Gly Met Met Lys Arg Phe Ala Glu Glu Lys Phe Leu Arg Arg 
            420                 425                 430         


Leu Lys Thr Ser Asp Asn Gly Ser Ile Tyr Tyr Gln Leu His Leu Glu 
        435                 440                 445             


Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys His His Pro Phe Leu 
    450                 455                 460                 


Leu Glu Glu Glu Lys Lys Ile Glu Ser Leu Val Ser Phe Arg Ile Pro 
465                 470                 475                 480 


Tyr Tyr Val Gly Pro Leu Ser Ser Lys Asn Ala Ser Leu Asp His Lys 
                485                 490                 495     


Gly Lys Gln Arg Phe Ala Trp Ser Glu Arg Arg Pro Gly Met Lys Asp 
            500                 505                 510         


Thr Val Ile Thr Pro Trp Asn Trp Asp Gln Ile Ile Asp Cys Asp Gln 
        515                 520                 525             


Ser Ala Glu Lys Phe Ile Leu Arg Met Thr Gly Asp Cys Thr Tyr Leu 
    530                 535                 540                 


Gln Gly Glu Pro Val Leu Pro Lys Cys Ser Leu Leu Tyr Glu Glu Tyr 
545                 550                 555                 560 


Cys Val Leu Asn Glu Leu Asn Gly Ala Arg Trp Thr Ile Asp Gly Asp 
                565                 570                 575     


Asp Trp Arg Arg Phe Asp Val Ala Gln Arg Glu Gly Ile Leu His Asp 
            580                 585                 590         


Leu Phe Arg Lys Thr Arg Ser Val Ala Tyr Asn Lys Val Ala Asp Trp 
        595                 600                 605             


Leu Glu Arg Glu Glu Asn Trp Ala Asn Ala His Val Arg Gly Gly Gln 
    610                 615                 620                 


Gly Glu Asn Ser Phe Glu Ser Lys Leu Gly Ser Tyr Ile Phe Phe Cys 
625                 630                 635                 640 


Lys Asp Val Phe Lys Thr Ala Ala Leu Asp Glu Ala Asp Tyr Pro Met 
                645                 650                 655     


Ile Glu Gln Ile Ile Leu Trp Asn Thr Leu Phe Glu Asp Arg Ala Ile 
            660                 665                 670         


Leu Lys Arg Lys Ile Glu Gln Ala Tyr Gly Glu His His Gly Gly Lys 
        675                 680                 685             


Leu Thr Asp Glu Gln Ile Lys Val Ile Cys Lys Lys Arg Phe Thr Gly 
    690                 695                 700                 


Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Lys Val Asp Ala 
705                 710                 715                 720 


Asp Thr Gly Arg Val Ser Ile Met Asp Val Leu Arg Glu Gly Asn Pro 
                725                 730                 735     


Asn Glu Gly Gln Arg Ser Arg Thr Met Val Leu Met Glu Val Leu Arg 
            740                 745                 750         


Asp Glu Asp Leu Gly Phe Gln Lys Lys Ile Asp Asp Phe Asn Lys Ala 
        755                 760                 765             


Tyr Phe Ala Gly Gln Gly Glu Gly Leu Met Ser Val Asn Asp Leu Pro 
    770                 775                 780                 


Gly Ser Pro Ala Ile Arg Arg Ser Ile Asn Gln Ala Ile Arg Ile Val 
785                 790                 795                 800 


Asp Glu Ile Ala Ser Ile Ala Gly Lys Ala Pro Ser Asn Val Phe Ile 
                805                 810                 815     


Glu Val Thr Arg Glu Glu Asp Asp Pro Arg Lys Lys Gly Arg Arg Thr 
            820                 825                 830         


Asn Arg Arg Tyr Glu Asp Ile Lys Asn Ala Leu Glu Asn Phe Lys Thr 
        835                 840                 845             


Glu Asp Gln Glu Leu Trp Ser Glu Leu Leu Gly Lys Ala Pro Asn Asp 
    850                 855                 860                 


Leu Asp Glu Arg Leu Ser Leu Tyr Phe Met Gln Arg Gly Lys Cys Met 
865                 870                 875                 880 


Tyr Ser Gly Arg Pro Ile Lys Leu Glu Glu Leu His Thr Gly Lys Tyr 
                885                 890                 895     


Glu Val Asp His Ile Ile Pro Arg Ser Tyr Ile Lys Asp Asp Ser Phe 
            900                 905                 910         


Glu Asn Lys Val Leu Val Cys Arg Glu Glu Asn Gln Arg Lys Thr Asn 
        915                 920                 925             


Glu Met Leu Ile Asp Pro Ala Ile Arg Arg Arg Met Gly Asp Tyr Trp 
    930                 935                 940                 


Arg Met Leu His Lys Ala Lys Leu Ile Gly Asp Lys Lys Phe Asn Asn 
945                 950                 955                 960 


Leu Phe Met Arg Asp Arg Ile Asp Asp Lys Thr Leu Lys Gly Phe Val 
                965                 970                 975     


Ala Arg Gln Leu Val Glu Thr Ser Gln Ser Val Lys Leu Val Gln Thr 
            980                 985                 990         


Leu Leu Glu Val Arg Tyr Pro Asn  Thr Lys Ile Val Pro  Val Lys Ala 
        995                 1000                 1005             


Asn Ile  Ser His Asp Leu Arg  Glu Ala Ala Asp Leu  Val Lys Cys 
    1010                 1015                 1020             


Arg Glu  Ala Asn Asp Phe His  His Ala His Asp Ala  Tyr Leu Ala 
    1025                 1030                 1035             


Cys Arg  Val Gly Leu Phe Ile  Gln Lys Arg His Pro  Ser Val Tyr 
    1040                 1045                 1050             


Ala Asn  Pro Ile Gly Leu Ser  Arg Val Met Arg Asp  Tyr Ala Arg 
    1055                 1060                 1065             


Lys Gln  Ala Gly Glu Phe Asn  Lys Thr His Ser Met  Ala His Thr 
    1070                 1075                 1080             


Lys Gly  Phe Ile Val Asn Ser  Phe Met Thr Ser Glu  Phe Asp Lys 
    1085                 1090                 1095             


Glu Thr  Gly Glu Ile Phe Trp  Asp Ala Glu Ala Ala  Val Glu Asp 
    1100                 1105                 1110             


Ile Arg  Arg Val Leu Asn Tyr  Arg Gln Cys Phe Ile  Ser Arg Met 
    1115                 1120                 1125             


Pro Val  Glu Asp Thr Gly Ala  Phe Trp Asp Gln Thr  Ile Tyr Ser 
    1130                 1135                 1140             


Pro Arg  Ser Asn Lys Arg Leu  Glu Leu Pro Leu Lys  Asp Asp Leu 
    1145                 1150                 1155             


Asp Pro  Asn Lys Tyr Gly Ser  Tyr Ser Arg Glu Gln  Phe Ala Tyr 
    1160                 1165                 1170             


Phe Phe  Ile Tyr Glu Ala Arg  Asp Ala Lys Lys Ser  Lys Thr Val 
    1175                 1180                 1185             


Leu Arg  Phe Ala Gln Val Pro  Val Arg Val Thr Ser  Ala Ile Ala 
    1190                 1195                 1200             


Arg Asp  Ser Ser Lys Leu Asp  Glu Tyr Ala Ser Gly  Leu Ala Val 
    1205                 1210                 1215             


Gln Glu  Gly Leu Glu Phe Thr  Lys Ile Ile Arg Arg  Arg Ile Leu 
    1220                 1225                 1230             


Lys Lys  Gln Leu Leu Glu Leu  Asn Gly Asp Arg Leu  Val Ile Thr 
    1235                 1240                 1245             


Gly Ala  Lys Glu Val Arg Asn  Ala Thr Glu Phe Ala  Phe Thr Leu 
    1250                 1255                 1260             


Glu Glu  Thr Ala Leu Val Ala  Met Cys Ser Glu Ile  Ala Arg Glu 
    1265                 1270                 1275             


Gly Val  Pro Ser Glu Ile Ala  Ser Asp Val Gln Ser  Ile Ser Ser 
    1280                 1285                 1290             


Gln Leu  Ser Asp Leu Phe Leu  Ala Leu Ile Glu Arg  Gly Leu Thr 
    1295                 1300                 1305             


Ile Gly  Val Arg Leu His Gly  Gln Leu Lys Leu Gly  Gln Tyr Leu 
    1310                 1315                 1320             


Arg Gln  Phe Glu Asp Ala Ala  Leu Arg Asp Lys Ala  Lys Val Ile 
    1325                 1330                 1335             


Ile Gly  Ile Ile Ser Ile Thr  Asn Gly Ala Thr Asn  Thr Leu Asp 
    1340                 1345                 1350             


Leu Ser  Ser Leu Asn Gly Ser  Lys Asn Val Gly Gln  Val Arg Ile 
    1355                 1360                 1365             


Thr His  Asn Lys Leu Leu Asn  Asp Lys Ser Thr Ser  Tyr Val Leu 
    1370                 1375                 1380             


Ile Asp  Gln Ser Val Thr Gly  Met Phe Glu Arg Arg  Thr Arg Ile 
    1385                 1390                 1395             


Gly Leu  
    1400 


<210>  1178
<211>  1400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1178

Met Lys Thr Ile Thr Glu Asp Tyr Ile Leu Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asn Ser Cys Gly Trp Ala Val Met Asp Lys Lys Asn Arg Leu Leu Arg 
            20                  25                  30          


Leu Arg Gly Lys Arg Ala Ile Gly Ser His Leu Phe Glu Glu Gly His 
        35                  40                  45              


Ser Ala Ala Asp Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Lys 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Arg Leu Leu Glu Glu Phe Phe Ala Glu Pro 
65                  70                  75                  80  


Met Ala Lys Val Asp Pro Ala Phe Phe Val Arg Leu His Gln Ser Trp 
                85                  90                  95      


Val Ser Pro Leu Asp Lys Asp Arg Lys Lys Tyr His Ala Ile Val Phe 
            100                 105                 110         


Pro Thr Ala Lys Glu Asp His Glu Phe Tyr His Asp Tyr Pro Thr Ile 
        115                 120                 125             


Tyr His Leu Arg Asp Ala Leu Met Arg Glu Asp Arg Gln Phe Asp Leu 
    130                 135                 140                 


Arg Glu Ile Phe Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn 
145                 150                 155                 160 


Phe Leu Gln Asp Thr Pro Val Asn Ser Phe Val Ala Ser Lys Ile Asp 
                165                 170                 175     


Val Ala Ser Phe Leu Thr Asn Val Asn Gln Ala Tyr Ala Asp Lys Tyr 
            180                 185                 190         


Gly Glu Glu Gln Asn Phe Ile Glu Leu Asn Glu Asn Asn Ala Ala Ala 
        195                 200                 205             


Ile Glu Ala Val Ile Arg Gly Lys Asp Lys Asp Lys Thr Thr Lys Gln 
    210                 215                 220                 


Leu Asp Lys Ala Lys Lys Ile Thr Lys Leu Leu Gln Ser Ser Thr Asn 
225                 230                 235                 240 


Lys Tyr Glu Lys Asn Val Thr Arg Gln Ile Ala Asn Ala Ile Ile Gly 
                245                 250                 255     


Tyr Lys Thr Gln Phe Glu Thr Ile Leu Gly Lys Asp Ile Asp Lys Asn 
            260                 265                 270         


Asp Lys Ala Asp Trp Glu Phe Lys Leu Ser Asp Gly Asp Ala Asp Asp 
        275                 280                 285             


Lys Leu Glu Asn Leu Leu Pro Glu Phe Asp Asp Asn Asp Gln Tyr Ile 
    290                 295                 300                 


Val Ala Glu Ile Lys Lys Leu Phe Gly Ser Ile Ala Leu Ser Gln Ile 
305                 310                 315                 320 


Val Tyr Glu Asn Met Thr Leu Ser Gln Ser Met Lys Cys Lys Tyr Lys 
                325                 330                 335     


Asp His Gly Glu Asp Tyr Asp Leu Leu Lys Gln Tyr Ile Ala Thr Leu 
            340                 345                 350         


Ser Asp His Ser Gln Val Arg Lys Leu Leu Leu Ala Tyr Asp Leu Tyr 
        355                 360                 365             


Val Asn Asn Arg His Gly Arg Leu Leu Glu Ala Lys Lys Glu Phe Lys 
    370                 375                 380                 


Gln Asp Lys Ala Leu Thr Gln Asp Glu Phe Tyr Lys Ile Val Lys Glu 
385                 390                 395                 400 


Asn Leu Asp Asp Ser Gln Leu Ala Gln Lys Ile Leu Gln Lys Ile Asp 
                405                 410                 415     


Glu Gly Lys Phe Met Pro Lys Gln Arg Val Asn Lys Asn Gly Tyr Ile 
            420                 425                 430         


Pro Tyr Gln Leu His Gln Ile Glu Leu Asp Gln Ile Ile Ala Asn Gln 
        435                 440                 445             


Ser Lys Tyr Tyr Pro Phe Leu Ala Glu Glu Asn Pro Val Lys Glu His 
    450                 455                 460                 


Arg Lys Gln Ala Pro Tyr Lys Leu Asp Glu Leu Val Arg Phe Arg Val 
465                 470                 475                 480 


Pro Tyr Tyr Val Gly Pro Leu Ile Ser Pro Asp Asp Lys Arg Lys Asp 
                485                 490                 495     


Glu Gln Gln Asn Lys Asn His Glu Met Phe Lys Phe Ala Trp Met Lys 
            500                 505                 510         


Arg Lys Asp Asp Asn Gly Ala Ile Thr Pro Trp Asn Phe Ala Gln Lys 
        515                 520                 525             


Val Asp Arg Glu Thr Ser Ala Asn Arg Phe Ile Lys Arg Met Thr Val 
    530                 535                 540                 


Lys Asp Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Ala Arg Ser Leu 
545                 550                 555                 560 


Leu Tyr Gln Lys Phe Glu Val Leu Asn Glu Leu Asn Asn Ile Arg Val 
                565                 570                 575     


Asn Gly Lys Arg Leu Glu Pro Lys Phe Lys Gln Lys Val Phe Asn Glu 
            580                 585                 590         


Leu Phe Lys Gln Lys Asn Lys Val Thr Lys Lys Ala Phe Lys Glu Phe 
        595                 600                 605             


Ile Leu Gln Asn Thr Gly Met Lys Thr Ala Glu Ile Asp Gly Leu Ala 
    610                 615                 620                 


Asp Pro Thr Lys Phe Asn Ser Ser Leu Ser Ser Tyr Tyr His Leu Lys 
625                 630                 635                 640 


Ser Thr Lys Val Phe Asp Lys Gln Leu Asp Asp Pro Gln Tyr Gln Ala 
                645                 650                 655     


Asp Phe Glu Lys Ile Ile Glu Tyr Ser Ser Ile Phe Glu Asp Ala Glu 
            660                 665                 670         


Ile Tyr Gln Glu Lys Leu His Lys Glu Ile Lys Trp Leu Thr Pro Ala 
        675                 680                 685             


Gln Phe Lys Ala Ile Ala Asn Trp Arg Leu Gln Gly Trp Gly Arg Leu 
    690                 695                 700                 


Ser Arg Lys Leu Leu Val Glu Leu Lys Gly Lys Gly Gly Glu Asn Ile 
705                 710                 715                 720 


Ile Tyr Gln Leu Trp Thr Thr Gln Lys Asn Phe Met Gln Ile Val Asn 
                725                 730                 735     


Glu Pro Glu Leu Lys Lys Ala Ile Val Lys Glu Asn His Gln Ala Thr 
            740                 745                 750         


Glu Glu Lys Gly Ile Glu Lys Ile Leu Asp Asp Ser Phe Thr Ser Pro 
        755                 760                 765             


Ala Asn Lys Lys Ala Ile Arg Gln Val Ile Lys Val Val Asp Asp Val 
    770                 775                 780                 


Val Lys Ala Ala Gly Gly Lys Lys Pro Ala Gln Phe Ala Ile Glu Phe 
785                 790                 795                 800 


Thr Arg Glu Pro Lys Trp Asn Pro His Leu Ser Asn Ile Arg Gly Thr 
                805                 810                 815     


Lys Leu Leu Gln Val Tyr Glu Asp Thr Ala Lys Lys Leu Val Asp Gln 
            820                 825                 830         


Ser Leu Thr Asp Ser Leu Arg Glu Asn Met Thr Asn Arg Asn Leu Leu 
        835                 840                 845             


Lys Asp Glu Tyr Phe Leu Tyr Phe Ile Gln Gly Gly Arg Asp Ala Tyr 
    850                 855                 860                 


Thr Gly Lys Lys Ile Asp Ile Asp Asp Ile Pro Lys Lys Tyr Gln Ile 
865                 870                 875                 880 


Asp His Ile Leu Pro Gln Ser Phe Ile Thr Asp Asp Ser Leu Asp Asn 
                885                 890                 895     


Arg Val Leu Thr Glu Ile Glu Ile Asn Gln Lys Lys Gly Lys Lys Tyr 
            900                 905                 910         


Ala Ser His Leu Phe Ala Pro Lys Pro Ala Phe Gly Leu Asn Gln Ser 
        915                 920                 925             


Ile Ser Glu Met Trp Lys Thr Trp Gln Lys Ala Gly Leu Ile Ser Gln 
    930                 935                 940                 


Arg Lys Leu Asn Asn Leu Leu Phe Asp Pro Asp Leu Val Lys Asp Lys 
945                 950                 955                 960 


Gln Lys Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Ile 
                965                 970                 975     


Ile Lys Leu Val Ser Ile Ile Leu Gln Asp Lys Phe Pro Asp Thr Glu 
            980                 985                 990         


Ile Ile Thr Val Lys Ala Gly Tyr  Asn Thr Ala Leu Arg  Lys Glu Leu 
        995                 1000                 1005             


Lys Leu  Tyr Lys Ser Arg Glu  Ala Asn Asp Tyr His  His Ala Ile 
    1010                 1015                 1020             


Asp Ala  Tyr Leu Thr Ala Ile  Cys Asp Asn Phe Leu  Tyr Gln Val 
    1025                 1030                 1035             


Tyr Pro  Asn Phe Arg Pro Phe  Phe Val Tyr Gly Gln  Phe Lys Lys 
    1040                 1045                 1050             


Phe Ser  Gln Asp Thr Asp Lys  Gln Lys Lys Ala Val  Lys Tyr Tyr 
    1055                 1060                 1065             


Gly Leu  Lys Asn Phe Leu Leu  Pro Leu Leu Asp Asp  Ser Thr Gly 
    1070                 1075                 1080             


Asp Glu  Val Met Val Asn His  Thr Lys His Val Val  Phe His Lys 
    1085                 1090                 1095             


Gln Arg  Asp Ile Phe Asn Arg  Leu Ile Gln Ala Tyr  Asn Tyr Lys 
    1100                 1105                 1110             


Tyr Met  Leu Val Ser Arg Glu  Thr Thr Thr Glu Asn  Ser Asn Met 
    1115                 1120                 1125             


Phe Asn  Met Thr Ile Tyr Pro  Arg Gly Glu Arg Asp  Arg Lys Lys 
    1130                 1135                 1140             


Thr Arg  Lys Leu Ile Pro Lys  Gly Lys Asp Leu Asp  Pro Ala Ile 
    1145                 1150                 1155             


Tyr Gly  Gly Tyr Ser Gly Asn  Thr Asp Ala Tyr Met  Val Ile Val 
    1160                 1165                 1170             


Lys Ile  Glu Lys Thr Lys Glu  Thr Leu Tyr Lys Val  Val Gly Val 
    1175                 1180                 1185             


Pro Met  Arg Ala Leu Ala Ser  Leu Lys Lys Ala Gln  Lys Glu Gly 
    1190                 1195                 1200             


Asn Tyr  Asp Gln Glu Leu Asn  Lys Val Leu Glu Pro  Leu Ile Met 
    1205                 1210                 1215             


Phe Asn  Lys Asn Gly Lys Ser  Lys Ala Gly Ile Lys  Gly Phe Thr 
    1220                 1225                 1230             


Val Val  Lys Asp His Val His  Phe Lys Gln Val Val  Ile Asp Gly 
    1235                 1240                 1245             


Asp Ser  Lys Phe Met Leu Tyr  Ser Ala Gln Tyr Arg  Ala Asn Ala 
    1250                 1255                 1260             


Lys Gln  Leu Thr Leu Ser Gln  Glu Thr Met Lys Val  Val Thr Asp 
    1265                 1270                 1275             


Asn Thr  Asp Asp Met Asp Gln  Lys Asn Ala Asp Lys  Asp Gln Leu 
    1280                 1285                 1290             


Leu Leu  Asn Ala Tyr Asp Glu  Ile Leu Glu Lys Val  Asp Gln Tyr 
    1295                 1300                 1305             


Leu Pro  Leu Phe Asp Ile Asn  Gly Phe Arg Gln Gly  Leu His Asp 
    1310                 1315                 1320             


Gly Arg  Ala Lys Phe Ala Ala  Leu Lys Thr Ser Glu  Lys Lys Tyr 
    1325                 1330                 1335             


Thr Leu  Lys Asn Val Leu Asn  Gly Leu His Asp Asn  Leu Val Thr 
    1340                 1345                 1350             


Pro Asn  Leu Lys Asn Ile Gly  Ile Lys Thr Pro Phe  Gly Leu Met 
    1355                 1360                 1365             


Gln Ser  Ser Ser Gly Ile Thr  Leu Ser Pro Asn Ala  Lys Leu Val 
    1370                 1375                 1380             


Phe Gln  Ser Pro Thr Gly Leu  Phe Glu Lys Arg Val  Lys Leu Thr 
    1385                 1390                 1395             


Asn Leu  
    1400 


<210>  1179
<211>  1400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1179

Met Gly Lys Gln Lys Phe Asn Asp Tyr Tyr Leu Gly Phe Asp Ile Gly 
1               5                   10                  15      


Thr Asn Ser Ala Gly Trp Ala Val Thr Asp Leu Gln Tyr Lys Leu Leu 
            20                  25                  30          


Arg Phe Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Asp Glu Ala 
        35                  40                  45              


Gln Thr Ser Lys Glu Arg Arg Val Tyr Arg Asn Ser Arg Arg Arg Leu 
    50                  55                  60                  


Asp Arg Arg Lys Lys Arg Leu Lys Leu Leu Glu Glu Leu Phe Leu Asp 
65                  70                  75                  80  


Glu Ile Ser Lys Ile Asp Asn Thr Phe Phe Lys Arg Leu Lys Glu Ser 
                85                  90                  95      


Asn Leu Tyr Asn Glu Asp Lys Ser Ile Lys Phe Asn Tyr Pro Leu Phe 
            100                 105                 110         


Asn Asp Lys Asp Tyr Thr Asp Ile Asp Phe Tyr Asn Glu Tyr Lys Thr 
        115                 120                 125             


Ile Tyr His Leu Arg Lys Asp Leu Ile Phe Glu Asp Lys Lys Phe Asp 
    130                 135                 140                 


Ile Arg Leu Val Phe Ile Ala Leu His His Ile Leu Lys Tyr Arg Gly 
145                 150                 155                 160 


His Phe Ile Phe Glu Gly Gln Ser Phe Glu Glu Ile Lys Ser Phe Asp 
                165                 170                 175     


Ser Ile Phe Thr Gln Leu Ser Ser Tyr Phe Leu Glu Asn Phe Asp Phe 
            180                 185                 190         


Asn Ile Asp Val Ser Ile Lys Glu Lys Leu Glu Glu Ile Ile Leu Ser 
        195                 200                 205             


Asp Asn Thr Lys Ser Ile Lys Lys Thr Glu Phe Lys Lys Ile Phe Ser 
    210                 215                 220                 


Asp Asn Lys Val Leu Ser Val Leu Asn Met Ala Ile Gly Ser Lys Val 
225                 230                 235                 240 


Ser Leu Lys Asp Val Phe Asn Asn Glu Glu Leu Lys Glu Gln Lys Ile 
                245                 250                 255     


Ser Phe Ser Glu Ile Asp Tyr Glu Asn Glu Lys Arg Asp Glu Phe Tyr 
            260                 265                 270         


Gln Leu Leu Gly Glu Asp Ile Phe Leu Ile Asp Leu Ala Lys Lys Ile 
        275                 280                 285             


Tyr Asp Phe Met Val Leu Lys Lys Ile Leu Lys Asn Thr Asp Ser Phe 
    290                 295                 300                 


Ser Ala Ser Lys Val Ala Leu Tyr Cys Glu His Lys Lys Asp Leu Asp 
305                 310                 315                 320 


Asp Leu Lys Tyr Ile Phe Lys Lys Tyr Leu Thr Lys Glu Asp Tyr Phe 
                325                 330                 335     


Lys Phe Phe Arg Ser Asn Val Glu Lys Ile Asn Tyr Pro Ser Tyr Ile 
            340                 345                 350         


Gly Leu Asn Lys Ile Lys Ser Lys Lys Ile Ile Lys Glu Asn Lys Cys 
        355                 360                 365             


Ser Ile Ser Asp Ile Asn Lys Glu Leu Lys Asn Lys Tyr Leu Lys Asp 
    370                 375                 380                 


Ile Lys Leu Glu Ala Lys Asp Lys Glu Leu Phe Asn Arg Ile Phe Asn 
385                 390                 395                 400 


Lys Leu Asp Glu Phe Lys Phe Leu Glu Lys Leu Lys Ser Ser Glu Asn 
                405                 410                 415     


Ser Thr Val Pro Tyr Gln Val His Glu Ile Glu Leu Lys Lys Ile Leu 
            420                 425                 430         


Asp Asn Gln Arg Lys Tyr Tyr Pro Phe Leu Asn Glu Lys Ile Asp Gly 
        435                 440                 445             


Lys Ser Val Tyr Glu Lys Ile Ile Gln Leu Phe Glu Phe Lys Ile Pro 
    450                 455                 460                 


Tyr Tyr Ile Gly Pro Leu Asn Thr Asp Pro Asn Val Lys Gly Lys Asn 
465                 470                 475                 480 


Thr Trp Val Val Arg Lys Asp Glu Asn Asn Lys Thr Pro Ile Thr Pro 
                485                 490                 495     


Trp Asn Phe Glu Gln Lys Val Asp Met Gln Lys Ser Ala Glu Glu Phe 
            500                 505                 510         


Ile Lys Arg Met Thr Asn Arg Cys Thr Tyr Leu Lys Ala Glu Asp Val 
        515                 520                 525             


Ile Ala Lys Asn Ser Leu Leu Tyr Glu Glu Phe Ile Leu Leu Asn Glu 
    530                 535                 540                 


Leu Asn Lys Val Thr Val Asp Asn Glu Ile Leu His Pro Thr Leu Lys 
545                 550                 555                 560 


Thr Glu Ile Ile Asn Glu Leu Phe Lys Thr Thr Val Lys Val Thr Lys 
                565                 570                 575     


Lys Lys Phe Ile Glu Phe Leu Lys Lys Lys Gln Ala Val Asn Asp Val 
            580                 585                 590         


Glu Ser Ile Thr Gly Ile Gly Val Glu Phe Asn Cys Lys Leu Gln Gly 
        595                 600                 605             


Tyr Asn Thr Phe Arg Lys Ile Leu Gly Lys Lys Ile Glu Asn Val Asn 
    610                 615                 620                 


Val Lys Asn Phe Val Glu Lys Cys Ile Glu Leu Lys Cys Leu Tyr Gly 
625                 630                 635                 640 


Ser Asp Lys Lys Leu Phe Glu Asn Ala Ile Gly Lys Ile Tyr Arg Glu 
                645                 650                 655     


Tyr Asn Ile Thr Asp Glu Glu Phe Asn Lys Ile Asn Arg Leu Asn Phe 
            660                 665                 670         


Asn Lys Trp Ala Arg Leu Ser Lys Lys Phe Leu Thr Glu Ile Ser Phe 
        675                 680                 685             


Glu Lys Thr Asp Gly Thr Lys Arg Phe Glu Ser Val Ile Glu Ala Leu 
    690                 695                 700                 


Arg Ser Thr Asn Tyr Asn Leu Met Glu Leu Leu Ser Asp Lys Phe Asn 
705                 710                 715                 720 


Leu Gln Ser Lys Ile Asp Glu Ile Asn Gly Glu Asp Asp Phe Ser Asn 
                725                 730                 735     


Ile Asp Ile Lys Glu Tyr Val Asp Lys Leu Tyr Val Ser Pro Ser Val 
            740                 745                 750         


Lys Arg Ser Ile Ile Gln Thr Tyr Lys Ile Ala Met Glu Ile Lys Lys 
        755                 760                 765             


Ile Thr Lys Lys Asp Pro Lys Lys Ile Phe Ile Glu Met Ala Lys Asp 
    770                 775                 780                 


Lys Gly Glu Lys Gly Lys Thr Thr Glu Lys Arg Lys Asp Lys Ile Lys 
785                 790                 795                 800 


Lys Leu Tyr Lys Asn Ile Lys Asp Thr Leu Ser Asp Tyr Cys Asn Asn 
                805                 810                 815     


Leu Gln Asp Leu Thr Leu Gln Val Glu Lys Tyr Asp Asp Asn Arg Leu 
            820                 825                 830         


Arg Gln Lys Lys Leu Phe Leu Tyr Phe Met Gln Met Gly Lys Ser Met 
        835                 840                 845             


Tyr Thr Gly Arg Cys Ile Asp Leu Glu Asp Leu Phe Asn Asn Asn Val 
    850                 855                 860                 


Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Lys Asp Asp Ser 
865                 870                 875                 880 


Phe Asp Asn Ile Val Leu Val Glu Lys Glu Glu Asn His Arg Lys Ser 
                885                 890                 895     


Asn Glu Leu Leu Lys Glu Glu Ile Gln Ser Arg Met Asn Asn Tyr Trp 
            900                 905                 910         


Ile Phe Leu Arg Glu Asn Lys Phe Ile Ser Lys Glu Lys Tyr Asn Arg 
        915                 920                 925             


Leu Thr Arg Lys Lys Asp Phe Thr Asp Glu Glu Leu Gly Asp Phe Val 
    930                 935                 940                 


Ala Arg Gln Leu Val Thr Thr Arg Gln Ser Thr Lys Glu Ile Ala Lys 
945                 950                 955                 960 


Phe Phe Glu Lys Ile Phe Glu Lys Thr Thr Val Val Tyr Ser Lys Ala 
                965                 970                 975     


Ser Leu Val Ser Glu Phe Arg Glu Lys Tyr Asp Ile Ile Lys Val Arg 
            980                 985                 990         


Glu Val Asn Asp Ala His His Ala  Gln Asp Ala Tyr Leu  Asn Ile Val 
        995                 1000                 1005             


Val Gly  Asn Val Phe Asn Thr  Lys Phe Thr Lys Asn  Tyr Trp Lys 
    1010                 1015                 1020             


Phe Thr  Lys Asp Ile Lys Arg  Gln Asn Glu Glu Asn  Pro Asn Glu 
    1025                 1030                 1035             


Lys Lys  Gly Tyr Asn Leu Arg  Lys Ile Tyr Glu Ser  Asp Val Phe 
    1040                 1045                 1050             


Cys Asn  Asp Ser Val Ala Trp  Val Tyr Ser Lys Glu  Asn Pro Lys 
    1055                 1060                 1065             


Tyr Asn  Thr Ile Asn Thr Val  Lys Asp Met Val Tyr  Arg Lys Thr 
    1070                 1075                 1080             


Ala Asn  Ile Thr Arg Met Leu  Val Glu Gly Lys Gly  Gln Leu Phe 
    1085                 1090                 1095             


Asn Leu  Thr Ile Lys Asp Lys  Thr Gln Leu Lys Lys  Asn Val Gly 
    1100                 1105                 1110             


Asn Ile  Val Ala Asn Thr Pro  Ile Ser Leu Lys Asn  Arg Lys Leu 
    1115                 1120                 1125             


His Leu  Asn Ser Val Glu Ile  Ala Lys Lys Tyr Gly  Tyr Phe Asp 
    1130                 1135                 1140             


Ser Leu  Asn Asn Ala Tyr Phe  Val Leu Phe Lys Tyr  Glu Glu Lys 
    1145                 1150                 1155             


Gly Lys  Glu Lys Ile Gly Phe  Asp Ser Ile Thr Ile  Ala Tyr Lys 
    1160                 1165                 1170             


Glu Arg  Leu Lys Lys Asn Leu  Asp Ile Glu Lys Phe  Leu Glu Asn 
    1175                 1180                 1185             


Lys Gly  Tyr Lys Asn Ser Lys  Leu Ile Arg Lys Ile  Phe Lys Asn 
    1190                 1195                 1200             


Gln Lys  Val Leu Ile Asn Gly  Tyr Pro Tyr Leu Leu  Lys Ser Phe 
    1205                 1210                 1215             


Asn Lys  Gln Ser Gln Ile Lys  Phe Asn Asn Phe Lys  Ile Leu Tyr 
    1220                 1225                 1230             


Leu Asn  Glu Asn Asn Leu Lys  Phe Ala Lys Asp Ala  Ile Lys Phe 
    1235                 1240                 1245             


Glu Lys  Asn Asn Ala Asn Val  Asp Leu Lys Lys Gln  Lys Tyr Ile 
    1250                 1255                 1260             


Val Ser  Phe Leu Lys Lys Lys  Lys Gln Asn Glu Ile  Asn Glu Thr 
    1265                 1270                 1275             


Tyr Glu  Glu Cys Ile Asp Arg  Leu Asn Asp Glu Phe  Asp Thr Leu 
    1280                 1285                 1290             


Phe Leu  Lys Leu Cys Asp Ile  Gln Lys Ser Ser Ile  Tyr Thr Asn 
    1295                 1300                 1305             


Tyr Lys  Leu Gln Asp Phe Ser  Ser Asn Lys Lys Ser  Ile Glu Ile 
    1310                 1315                 1320             


Lys Asp  Lys Phe Asp Ile Phe  Lys Ser Leu Asp Leu  Tyr Lys Lys 
    1325                 1330                 1335             


Ser Lys  Leu Ile Lys Glu Phe  Leu Asn Tyr Tyr Lys  Tyr Glu Lys 
    1340                 1345                 1350             


Asn Asp  Leu Asp Phe Ser Leu  Ile Asp Ile Asp Lys  Lys Ala Asn 
    1355                 1360                 1365             


Cys Ile  Gln Lys His Phe Thr  Glu Asp Leu Val Lys  Asp Phe Val 
    1370                 1375                 1380             


Ile Val  Asp Glu Ser Val Thr  Gly Leu Phe Val Lys  Lys Glu Arg 
    1385                 1390                 1395             


Leu Lys  
    1400 


<210>  1180
<211>  1400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1180

Met Lys Thr Ile Thr Glu Asp Tyr Ile Leu Gly Leu Asp Ile Gly Thr 
1               5                   10                  15      


Asn Ser Cys Gly Trp Ala Val Met Asp Lys Lys Asn Arg Leu Leu Arg 
            20                  25                  30          


Leu Arg Gly Lys Arg Ala Ile Gly Ser His Leu Phe Glu Glu Gly His 
        35                  40                  45              


Ser Ala Ala Asp Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Lys 
    50                  55                  60                  


Arg Arg Lys Trp Arg Leu Arg Leu Leu Glu Glu Ile Phe Ala Glu Pro 
65                  70                  75                  80  


Met Ala Lys Val Asp Pro Ala Phe Phe Val Arg Leu His Gln Ser Trp 
                85                  90                  95      


Val Ser Pro Leu Asp Lys Asp Arg Lys Lys Tyr His Ala Ile Val Phe 
            100                 105                 110         


Pro Thr Ala Lys Glu Asp His Glu Phe Tyr His Asp Tyr Pro Thr Ile 
        115                 120                 125             


Tyr His Leu Arg Asp Ala Leu Met Arg Glu Asp Arg Gln Phe Asp Leu 
    130                 135                 140                 


Arg Glu Ile Phe Leu Ala Ile His His Ile Val Lys Tyr Arg Gly Asn 
145                 150                 155                 160 


Phe Leu Gln Asp Thr Pro Val Asn Ser Phe Val Ala Ser Lys Ile Asp 
                165                 170                 175     


Val Ala Ser Phe Leu Thr Asn Val Asn Gln Ala Tyr Ala Asp Lys Tyr 
            180                 185                 190         


Gly Glu Glu Gln Asn Phe Ile Glu Leu Asn Glu Asn Asn Ala Ala Ala 
        195                 200                 205             


Ile Glu Ala Val Ile Arg Gly Lys Asp Lys Asp Lys Thr Thr Lys Gln 
    210                 215                 220                 


Leu Asp Lys Ala Lys Lys Ile Thr Lys Leu Leu Gln Ser Ser Thr Asn 
225                 230                 235                 240 


Lys Tyr Glu Lys Asn Val Thr Arg Gln Ile Ala Asn Ala Ile Ile Gly 
                245                 250                 255     


Tyr Lys Thr Gln Phe Glu Thr Ile Leu Gly Lys Asp Ile Asp Lys Asn 
            260                 265                 270         


Asp Lys Ala Asp Trp Glu Phe Lys Leu Ser Asp Gly Asp Ala Asp Asp 
        275                 280                 285             


Lys Leu Glu Asn Leu Leu Pro Glu Phe Asp Asp Asn Asp Gln Tyr Ile 
    290                 295                 300                 


Val Ala Glu Ile Lys Lys Leu Phe Gly Ser Ile Ala Leu Ser Gln Ile 
305                 310                 315                 320 


Val Tyr Glu Asn Met Thr Leu Ser Gln Ser Met Lys Cys Lys Tyr Lys 
                325                 330                 335     


Asp His Gly Asp Asp Tyr His Leu Leu Lys Gln Tyr Ile Ala Thr Leu 
            340                 345                 350         


Ser Asp His Gly Gln Val Arg Lys Leu Leu Leu Ala Tyr Asp Leu Tyr 
        355                 360                 365             


Val Asn Asn Arg His Gly Arg Leu Leu Glu Ala Lys Lys Glu Phe Lys 
    370                 375                 380                 


Gln Asp Lys Ala Leu Thr Gln Asp Glu Phe Tyr Lys Ile Val Arg Glu 
385                 390                 395                 400 


Asn Leu Asp Asn Ser Gln Leu Ala Gln Lys Ile Leu Gln Lys Ile Glu 
                405                 410                 415     


Glu Glu Lys Phe Met Pro Lys Gln Arg Val Asn Lys Asn Gly Phe Ile 
            420                 425                 430         


Pro Tyr Gln Leu His Gln Ile Glu Leu Asp Gln Ile Ile Ala Asn Gln 
        435                 440                 445             


Ser Lys Tyr Tyr Pro Phe Leu Ala Glu Glu Asn Pro Val Lys Glu His 
    450                 455                 460                 


Arg Lys Gln Ala Pro Tyr Lys Leu Asp Glu Leu Val Arg Phe Arg Val 
465                 470                 475                 480 


Pro Tyr Tyr Val Gly Pro Leu Ile Ser Pro Asp Asp Lys Arg Lys Asp 
                485                 490                 495     


Lys Gln Gln Asn Lys Asn His Glu Met Phe Lys Phe Ala Trp Met Lys 
            500                 505                 510         


Arg Lys Asp Asn Asn Gly Ala Ile Thr Pro Trp Asn Phe Ala Gln Lys 
        515                 520                 525             


Val Asp Arg Glu Thr Ser Ala Asn Arg Phe Ile Lys Arg Met Thr Val 
    530                 535                 540                 


Lys Asp Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Ala Arg Ser Leu 
545                 550                 555                 560 


Leu Tyr Gln Lys Phe Glu Val Leu Asn Glu Leu Asn Asn Ile Arg Val 
                565                 570                 575     


Asn Gly Lys Arg Leu Glu Pro Lys Phe Lys Gln Lys Val Phe Asn Glu 
            580                 585                 590         


Leu Phe Lys Gln Lys Asn Lys Val Thr Lys Lys Ala Phe Lys Glu Phe 
        595                 600                 605             


Ile Leu Gln Asn Thr Gly Met Lys Thr Ala Glu Ile Asp Gly Leu Ala 
    610                 615                 620                 


Asp Pro Thr Lys Phe Asn Ser Ser Leu Ser Ser Tyr Tyr His Leu Lys 
625                 630                 635                 640 


Ser Thr Lys Val Phe Asp Lys Gln Leu Asp Asp Pro Glu Tyr Gln Ala 
                645                 650                 655     


Asp Phe Glu Lys Ile Ile Glu Tyr Ser Ser Ile Phe Glu Asp Ala Glu 
            660                 665                 670         


Ile Tyr Gln Glu Lys Leu His Lys Glu Ile Lys Trp Leu Ile Pro Ala 
        675                 680                 685             


Gln Phe Lys Ala Ile Ala Asn Trp Arg Leu Gln Gly Trp Gly Arg Leu 
    690                 695                 700                 


Ser Arg Lys Leu Leu Val Glu Leu Lys Gly Lys Gly Gly Glu Asn Ile 
705                 710                 715                 720 


Ile Tyr Gln Leu Trp Thr Thr Gln Lys Asn Phe Met Gln Ile Val Asn 
                725                 730                 735     


Glu Pro Glu Leu Lys Lys Ala Ile Val Lys Glu Asn His Gln Ala Thr 
            740                 745                 750         


Glu Glu Lys Gly Ile Glu Lys Ile Leu Asp Asp Ser Phe Thr Ser Pro 
        755                 760                 765             


Ala Asn Lys Lys Ala Ile Arg Gln Val Ile Lys Val Val Asp Asp Val 
    770                 775                 780                 


Val Lys Ala Ala Gly Gly Lys Lys Pro Ala Gln Phe Ala Ile Glu Phe 
785                 790                 795                 800 


Thr Arg Glu Pro Lys Trp Asn Pro Gln Leu Ser His Ile Arg Gly Thr 
                805                 810                 815     


Lys Leu Leu Gln Val Tyr Glu Asp Thr Ala Lys Lys Leu Val Asp Gln 
            820                 825                 830         


Ser Leu Thr Asp Ser Leu Lys Glu Asn Met Thr Asn Arg Asn Leu Leu 
        835                 840                 845             


Lys Asp Glu Tyr Phe Leu Tyr Phe Ile Gln Cys Gly Arg Asp Ala Tyr 
    850                 855                 860                 


Thr Gly Lys Lys Ile Asp Ile Ala Asp Ile His Lys Lys Tyr Gln Ile 
865                 870                 875                 880 


Asp His Ile Leu Pro Gln Ser Phe Ile Thr Asp Asp Ser Leu Asp Asn 
                885                 890                 895     


Arg Val Leu Thr Glu Ile Glu Ile Asn Gln Lys Lys His Lys Lys Tyr 
            900                 905                 910         


Ala Ser His Leu Phe Ala Thr Lys Pro Ala Phe Gly Leu Asn Gln Ser 
        915                 920                 925             


Ile Ser Glu Met Trp Lys Thr Trp Gln Lys Ala Gly Leu Ile Ser Gln 
    930                 935                 940                 


Arg Lys Leu Asn Asn Leu Leu Phe Asp Pro Asp Leu Val Lys Asp Lys 
945                 950                 955                 960 


Gln Lys Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Ile 
                965                 970                 975     


Ile Lys Leu Val Ser Ile Ile Leu Gln Asp Lys Phe Pro Asp Thr Glu 
            980                 985                 990         


Ile Ile Thr Val Lys Ala Gly Tyr  Asn Thr Ala Leu Arg  Lys Glu Leu 
        995                 1000                 1005             


Lys Leu  Tyr Lys Ser Arg Glu  Ala Asn Asp Tyr His  His Ala Ile 
    1010                 1015                 1020             


Asp Ala  Tyr Leu Thr Ala Ile  Asn Asp Asn Phe Leu  Tyr Gln Val 
    1025                 1030                 1035             


Tyr Pro  Asn Phe Arg Pro Phe  Phe Val Tyr Gly Gln  Phe Lys Lys 
    1040                 1045                 1050             


Phe Ser  Gln Asp Thr Asp Lys  Gln Lys Glu Ala Ile  Lys Tyr Tyr 
    1055                 1060                 1065             


Gly Leu  Lys Asn Phe Leu Glu  Pro Leu Leu Asp Asp  Ser Thr Gly 
    1070                 1075                 1080             


Asp Glu  Val Met Val Asn His  Thr Lys His Val Val  Phe His Lys 
    1085                 1090                 1095             


Gln Arg  Asp Ile Phe Asn Arg  Leu Ile Gln Ala Tyr  Asn Tyr Lys 
    1100                 1105                 1110             


Tyr Met  Leu Val Ser Arg Glu  Thr Thr Thr Glu Asn  Ser Asn Met 
    1115                 1120                 1125             


Phe Asn  Met Thr Ile Tyr Pro  Arg Gly Glu Arg Asp  Arg Lys Lys 
    1130                 1135                 1140             


Thr Arg  Lys Leu Ile Pro Lys  Gly Lys Asp Leu Asp  Pro Ala Ile 
    1145                 1150                 1155             


Tyr Gly  Gly Tyr Ser Gly Asn  Thr Asp Ala Tyr Met  Val Ile Val 
    1160                 1165                 1170             


Lys Ile  Glu Lys Thr Lys Glu  Thr Leu Tyr Lys Val  Val Gly Val 
    1175                 1180                 1185             


Pro Met  Arg Ala Leu Ala Ser  Leu Lys Lys Ala Gln  Lys Glu Gly 
    1190                 1195                 1200             


Asn Tyr  Asp Gln Glu Leu Asn  Lys Val Leu Glu Pro  Leu Ile Met 
    1205                 1210                 1215             


Phe Asn  Lys Asn Gly Lys Ser  Lys Ala Gly Ile Lys  Gly Phe Thr 
    1220                 1225                 1230             


Val Val  Lys Asp His Val His  Phe Lys Gln Val Val  Ile Asp Gly 
    1235                 1240                 1245             


Asp Ser  Lys Phe Met Leu Tyr  Ser Ala Gln Tyr Arg  Ala Asn Ala 
    1250                 1255                 1260             


Lys Gln  Leu Thr Leu Ser Gln  Glu Thr Met Lys Val  Val Thr Asp 
    1265                 1270                 1275             


Asn Thr  Asp Asp Met Asp Gln  Lys Asn Ala Asp Lys  Asp Gln Leu 
    1280                 1285                 1290             


Leu Leu  Asn Ala Tyr Asp Glu  Ile Leu Glu Lys Val  Asp Gln Tyr 
    1295                 1300                 1305             


Leu Pro  Leu Phe Asp Ile Asn  Gly Phe Arg Gln Gly  Leu His Asp 
    1310                 1315                 1320             


Gly Arg  Ala Lys Phe Ala Ala  Leu Lys Thr Ser Glu  Lys Lys Tyr 
    1325                 1330                 1335             


Thr Leu  Lys Asn Ile Leu Asn  Gly Leu His Asp Asn  Leu Val Thr 
    1340                 1345                 1350             


Pro Asn  Leu Lys Asn Ile Gly  Ile Lys Thr Pro Phe  Gly Leu Met 
    1355                 1360                 1365             


Gln Ser  Ser Ser Gly Ile Thr  Leu Ser Pro Asn Ala  Lys Leu Val 
    1370                 1375                 1380             


Phe Gln  Ser Pro Thr Gly Leu  Phe Glu Lys Arg Val  Lys Leu Thr 
    1385                 1390                 1395             


Asn Leu  
    1400 


<210>  1181
<211>  1400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1181

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Glu Thr Glu Asn Ser Asn Glu Lys Ser Glu Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Ala Gly Arg Pro Leu Ser Thr Asn Ala Asp Arg Thr Ala Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Lys Asn 
65                  70                  75                  80  


Leu Ile Asp Leu Leu Ile Lys His Arg Leu Ile Asp Lys Asp Thr Pro 
                85                  90                  95      


Leu Thr Glu Ile Gly Lys Asn Thr Thr His Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Arg Glu Arg Ile Glu Leu Glu Asp Leu Ala Arg Val 
        115                 120                 125             


Phe Leu Ala Ile Asn Lys Lys Arg Gly Tyr Arg Ser Ser Arg Lys Val 
    130                 135                 140                 


Asn Asn Glu Glu Glu Gly Gln Val Val Asp Gly Met Ala Val Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Asp Glu Asn Leu Thr Pro Gly Gln Tyr Ala Tyr Glu Leu 
                165                 170                 175     


Leu Ser Lys Gly Lys Lys Tyr Val Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asp Ser Ile Trp Glu Tyr Gln Lys Gln Phe Tyr Ala 
        195                 200                 205             


Asp Ile Leu Asp Asp Glu Leu Tyr Asn Ala Leu Lys Gly Gln Gly Gln 
    210                 215                 220                 


Gln Asn Ser Arg Lys Arg Phe Leu Ala Ile Lys Gly Val Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Lys Arg Asp Glu Val Lys Leu Gln His Tyr Lys Trp 
                245                 250                 255     


Arg Ser Glu Ala Ile Thr Gln Lys Leu Ser Ile Glu Glu Val Ala Tyr 
            260                 265                 270         


Val Leu Val Glu Ile Asn Asn Asp Leu Asn Lys Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Glu Thr 
    290                 295                 300                 


Val Gly Glu Asn Leu Trp Lys Gln Ile Gln Lys Asn Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Glu Thr Gln Ala Gln Phe His Pro Gln Leu Thr Leu Ala 
            340                 345                 350         


Leu Lys Glu Gln Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys Gly Leu Leu Ser Phe Cys Gln Phe Glu Ser Trp Glu 
    370                 375                 380                 


Ile Glu Arg Lys Asp Glu Asn Gly Asn Val Ile Leu Asn Lys Thr Thr 
385                 390                 395                 400 


Gln Leu Pro Lys Arg Gln Thr Val Gly Arg Arg Val Ala Pro Lys Ser 
                405                 410                 415     


Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Asn Ile Asn Asn Leu 
            420                 425                 430         


Glu Ile Ala Lys Ile Ser Asp Gly Asn Ser Lys Asn Lys Lys Glu Thr 
        435                 440                 445             


Glu Ala Leu Ser Asp Asp Glu Arg Lys Leu Leu Phe Glu Glu Leu Asn 
    450                 455                 460                 


Leu Arg Gly Asn Leu Ser Gln Lys Glu Val Leu Gln Ile Leu Gly Leu 
465                 470                 475                 480 


Lys Asp Lys Glu Tyr Lys Thr Asn Phe Pro Glu Gly Leu Glu Gly Asn 
                485                 490                 495     


Arg Thr Asn Ala Ala Leu Phe Asn Ile Tyr Gln Gln Ile Ala Glu Asn 
            500                 505                 510         


Glu Gly Tyr Gly Asp Trp Thr Lys Lys Ser Ala Gln Glu Ile Lys Glu 
        515                 520                 525             


Glu Leu Lys Ala Val Phe Pro Gln Ile Gly Ile Gln Ala Asp Ile Leu 
    530                 535                 540                 


Asp Phe Asn Ala Glu Leu Asp Gly Lys Glu Phe Glu Asn Gln Ala Ser 
545                 550                 555                 560 


Tyr Gln Leu Trp His Leu Leu Tyr Ser Ala Glu Glu Asp Asp Lys Ile 
                565                 570                 575     


Asn Glu Glu Asp Gln Ile Ile Tyr Gly Asn Ser Ala Val Ser Leu Lys 
            580                 585                 590         


Lys Lys Leu Cys Glu Lys Phe Gly Phe Thr Pro Glu Tyr Ala Lys Trp 
        595                 600                 605             


Ile Ala Asn Val Ser Leu Gln Asp Asp Tyr Gly Asn Leu Ser Thr Lys 
    610                 615                 620                 


Ala Met Arg Lys Ile Ile Pro Tyr Leu Ile Asp Gly Asn Asp Tyr Ser 
625                 630                 635                 640 


Glu Ala Cys Ala Leu Ala Gly Tyr Asn His Ser Asn Ser Leu Thr Lys 
                645                 650                 655     


Glu Glu Asn Asp Asn Arg Glu Arg Leu Asn Lys Leu Glu Leu Leu Pro 
            660                 665                 670         


Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Met 
        675                 680                 685             


Val Asn Val Val Asn Gln Val Ile Glu Thr Tyr Gly Lys Pro Asp Glu 
    690                 695                 700                 


Val Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Ser Ala Glu Glu Arg 
705                 710                 715                 720 


Ala Glu Met Thr Lys Gly Ile Asn Glu Ala Thr Arg Arg Asn Glu Asp 
                725                 730                 735     


Ile Lys Lys Leu Ile Thr Lys Asn Phe Gly Ile Pro Asn Pro Thr Lys 
            740                 745                 750         


Asn Asp Val Val Arg Tyr Arg Leu Trp Glu Glu Leu Ala Pro Leu Ala 
        755                 760                 765             


Tyr Lys Asp Val Phe Thr Gly Arg Gln Ile Lys Lys Glu Asp Leu Phe 
    770                 775                 780                 


Ser Ser Lys Ile Asp Ile Glu His Ile Ile Pro Lys Ala Leu Leu Phe 
785                 790                 795                 800 


Asp Asp Ser Phe Ser Asn Lys Thr Leu Ala Phe Arg Glu Thr Asn Leu 
                805                 810                 815     


Lys Lys Ala Asp Arg Thr Ala Tyr Gly Phe Ile Glu Ser Asp Tyr Asn 
            820                 825                 830         


Ala Thr Leu Asp Asp Tyr Ile Gln Arg Val Glu Thr Leu Tyr Asn Asn 
        835                 840                 845             


Ala Lys Gly Thr Ile Ser Lys Gly Lys Arg Asn Lys Leu Leu Met Ala 
    850                 855                 860                 


Gln Lys Asn Leu Pro Asp Gly Phe Ile Glu Arg Asp Leu Arg Asn Ser 
865                 870                 875                 880 


Gln Tyr Ile Ala Lys Lys Ala Lys Ser Met Ile Glu Lys Val Phe Asn 
                885                 890                 895     


Asp Val Asn Val Thr Ser Gly Ser Ile Thr Asp Arg Leu Arg Glu Asp 
            900                 905                 910         


Trp Asp Leu Ile Asn Val Met Lys Glu Leu Asn Phe Pro Lys Tyr Lys 
        915                 920                 925             


Ala Leu Gly Leu Thr Glu Ile Glu Glu Arg Tyr Asp Ile Gly Gln Glu 
    930                 935                 940                 


Lys Thr Lys Lys Val Glu Val Ile Thr Asp Trp Thr Lys Arg Asn Asp 
945                 950                 955                 960 


His Arg His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Thr Lys 
                965                 970                 975     


Ser His Val Gln Tyr Leu Asn Asn Leu Asn Thr Ile His Ser Leu Lys 
            980                 985                 990         


Gly Asp Glu Val Asp Leu Glu Leu  Ser Lys Leu Tyr Gly  Leu Lys Asn 
        995                 1000                 1005             


Thr Ile  Thr Glu Val Val Asn  Lys Lys Arg Lys Phe  Ile Pro Pro 
    1010                 1015                 1020             


Met Pro  Asn Phe Arg Glu Glu  Ala Lys Lys His Ile  Glu Thr Ile 
    1025                 1030                 1035             


Leu Val  Ser Ile Lys Asn Lys  Asn Lys Val Tyr Thr  Lys Asn Ile 
    1040                 1045                 1050             


Asn Lys  Thr Lys Lys Glu Ser  Gly Tyr His Thr Lys  Glu Gln Leu 
    1055                 1060                 1065             


Thr Pro  Arg Gly Gln Leu His  Lys Glu Thr Val Tyr  Gly Lys Ser 
    1070                 1075                 1080             


Lys Arg  Pro Met Asn Lys Pro  Thr Lys Ile Asn Lys  Asn Phe Ser 
    1085                 1090                 1095             


Leu Glu  Gln Ala Gln Leu Ile  Ile Asn Val Gln Glu  Lys Glu Leu 
    1100                 1105                 1110             


Val Leu  Arg His Leu Ala Gln  Phe Asp Asn Asn Pro  Ala Val Ala 
    1115                 1120                 1125             


Phe Asp  Thr Lys Thr Leu Lys  Lys Met Pro Leu Leu  Lys Asn Gly 
    1130                 1135                 1140             


Glu Pro  Leu Lys Glu Val Leu  Cys Phe Glu Glu Ile  Phe Thr Ile 
    1145                 1150                 1155             


Arg Lys  Asp Ile Thr Pro Asp  Leu Lys Ile Glu Lys  Val Val Asp 
    1160                 1165                 1170             


Glu Lys  Ile Lys Ala Ile Leu  Glu Gln Arg Lys Lys  Asp Phe Gly 
    1175                 1180                 1185             


Gly Asn  Ala Lys Glu Ala Phe  Ser Asp Leu Asp Lys  Asn Pro Ile 
    1190                 1195                 1200             


Trp Leu  Asn Lys Glu Lys Gly  Ile Cys Ile Lys Arg  Val Thr Ile 
    1205                 1210                 1215             


Thr Gly  Val Ser Asn Ala Glu  Ala Leu His His Gln  Lys Asp His 
    1220                 1225                 1230             


Leu Gly  Arg Glu Ile Leu Asp  Asp Asn Gly Asn Pro  Ile Pro Ala 
    1235                 1240                 1245             


Asp Phe  Val Ser Thr Gly Asn  Asn His His Val Ala  Ile Tyr Glu 
    1250                 1255                 1260             


Asp Glu  Lys Gly Lys Leu Gln  Glu Lys Val Val Ser  Leu Tyr Glu 
    1265                 1270                 1275             


Ala Val  Ala Arg Ala Asn Gln  Gly Leu Pro Val Val  Asp Lys Gly 
    1280                 1285                 1290             


Leu Asn  Glu Asp Leu Gly Trp  Lys Phe Leu Phe Thr  Met Lys Gln 
    1295                 1300                 1305             


Asn Glu  Met Phe Val Phe Pro  Asn Asp Asp Phe Asp  Val Asn Asp 
    1310                 1315                 1320             


Ile Asp  Leu Leu Asp Glu Asn  Asn Ala Lys Ile Ile  Ser Gln His 
    1325                 1330                 1335             


Leu Phe  Arg Val Gln Lys Leu  Ala Thr Lys Asn Tyr  Met Phe Arg 
    1340                 1345                 1350             


His His  Leu Glu Thr Asn Val  Gln Glu Val Lys Glu  Leu Asn Asp 
    1355                 1360                 1365             


Ile Ala  Tyr Lys Ser Ile Arg  Ser Thr Glu Pro Leu  Lys Asn Ile 
    1370                 1375                 1380             


Lys Lys  Val Arg Ile Asn His  Ile Gly Lys Ile Val  Gln Val Gly 
    1385                 1390                 1395             


Glu Tyr  
    1400 


<210>  1182
<211>  1402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1182

Met Ala Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser 
1               5                   10                  15      


Val Gly Trp Ala Ile Ile Asp Asp Asn Leu Lys Leu Leu Lys Arg Asn 
            20                  25                  30          


Met Thr Ile Lys Gly Asn Thr Asp Lys Lys Ser Val Lys Arg Asp Leu 
        35                  40                  45              


Trp Gly Ser Leu Leu Tyr Ser Gly Asn Ser Asp Lys Thr Thr Ser Ala 
    50                  55                  60                  


Ala Asp Ala Arg Ser Lys Arg Gly Leu Arg Arg Arg Leu Arg Arg Arg 
65                  70                  75                  80  


Lys Tyr Arg Leu Asp Arg Leu Lys Gln Ile Phe Ser Glu Ile Ile Asn 
                85                  90                  95      


Asp Lys Ala Pro Asn Phe Phe Asp Lys Leu Asn Glu Ser Phe Leu Asn 
            100                 105                 110         


Pro Lys Asp Lys Lys Tyr Gly Lys Tyr Gln Ile Phe Asp Thr Glu Lys 
        115                 120                 125             


Glu Glu Lys Asp Tyr Tyr Arg Arg Tyr Pro Thr Ile Tyr His Leu Arg 
    130                 135                 140                 


Lys Asp Leu Ile Glu Ser Ser Lys Lys Gln Asp Ile Arg Leu Val Tyr 
145                 150                 155                 160 


Leu Ala Leu Ala His Ile Leu Lys Ser Arg Gly Asn Phe Leu Phe Glu 
                165                 170                 175     


Gly Asn Ile Asp Asp Leu Lys Asn Asp Phe Ala Gly Ile Tyr Glu Glu 
            180                 185                 190         


Val Val Glu Leu Cys Met Thr Ile Asn Ala Glu Asp Val Asp Leu Glu 
        195                 200                 205             


Phe Glu Glu Val Asp Lys Gln Ser Leu Asn Ser Ile Ile Lys Asn Glu 
    210                 215                 220                 


Asp Ile Ser Glu Ile Glu Gln Gly Leu Glu Asn Phe Ala Asp Glu His 
225                 230                 235                 240 


Val Ile Phe Lys Glu Gln Asn Lys Lys Lys Asn Asp Leu Phe Ser Asn 
                245                 250                 255     


Cys Cys Lys Ile Ile Cys Gly His Thr Val Lys Ala Asn Lys Phe Ala 
            260                 265                 270         


Ser Glu Leu Asp Ser Glu Leu Phe Ile Ser Phe Lys Ser Asp Asp Tyr 
        275                 280                 285             


Val Asp Val Ile Asp Val Ile Gln Ser Gly Asn Glu Asn Ile Ala Asn 
    290                 295                 300                 


Leu Leu Leu Ala Cys Arg Lys Ala Tyr Asp Tyr Ile Met Phe Asn Arg 
305                 310                 315                 320 


Leu Val Asp Leu Asn Ile Asp Ser Pro Ala Lys Leu Ser Ser Asn Met 
                325                 330                 335     


Val Ser Leu Tyr Asn Gln His Glu Lys Asp Leu Lys Ala Tyr Lys Lys 
            340                 345                 350         


Leu Ile Lys Glu Phe Asn Lys Phe Lys Arg Ser Asn Gly Cys Lys Asp 
        355                 360                 365             


Leu Glu Met Ile Ile Leu Thr Ala Asp Asp Ile Asp Ser Phe Arg Lys 
    370                 375                 380                 


Lys Val Asp Lys Lys Glu Gly Lys Leu Asn Gly Ile Asn Lys Lys Ile 
385                 390                 395                 400 


Thr His Glu Gln Ala Leu Lys Lys Gln Leu Lys Asp Met Lys Lys Ile 
                405                 410                 415     


Leu Glu Asp Lys Asn Thr Glu Ala Glu Asp Lys Gln Ile Asn Asp Ile 
            420                 425                 430         


Leu Lys Met Ile Thr Ser Ile Glu Glu Arg Val Asn Lys Ser Cys Phe 
        435                 440                 445             


Leu Lys Asn Leu Arg Ser Thr Asp Asn Ala Ser Ile Pro Asn Gln Ile 
    450                 455                 460                 


Gln Arg Gln Glu Met Glu Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr 
465                 470                 475                 480 


Pro Phe Leu Asn Glu His Lys Asp Glu Leu Leu Gln Leu Leu Ser Phe 
                485                 490                 495     


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Val Asn Lys Lys Tyr Ser Arg 
            500                 505                 510         


Phe Ala Trp Leu Val Arg Lys Glu Gly Gln Val Gln Lys Ile Thr Pro 
        515                 520                 525             


Thr Asn Phe Asp Gly Val Val Asp Lys His Lys Thr Ala Glu Lys Phe 
    530                 535                 540                 


Met Glu Arg Leu Ile Gly Lys Asp Val Tyr Leu Pro Asn Glu Arg Val 
545                 550                 555                 560 


Leu Pro Lys Ala Ser Leu Leu Tyr Gln Glu Tyr Cys Ile Phe Asn Glu 
                565                 570                 575     


Leu Thr Lys Val Ala Tyr Ile Asp Ser Thr Gly Lys Lys Asn Asn Phe 
            580                 585                 590         


Ser Ser Glu Glu Lys Leu Asn Ile Phe Glu Lys Leu Phe Lys Thr Lys 
        595                 600                 605             


Arg Glu Val Thr Lys Thr Asp Leu Cys Lys Cys Leu Asn Asn Val Cys 
    610                 615                 620                 


Lys Leu Lys Glu Lys Val Lys Glu Thr Glu Ile Ile Gly Ile Lys Ala 
625                 630                 635                 640 


Lys Phe Asn Ala Lys Tyr Ser Thr Tyr His Asp Leu Lys Lys Ile Asn 
                645                 650                 655     


Gly Met Glu Gln Leu Ile Ala Asp Glu Glu Gly Lys Pro Leu Cys Glu 
            660                 665                 670         


Asp Ile Ile Ser Ile Leu Thr Ile Phe Glu Asp Lys Asp Ile Arg Leu 
        675                 680                 685             


Val Arg Leu Lys Glu Leu Leu Cys Gln Asn Lys Asp Leu Ile Asn Lys 
    690                 695                 700                 


Phe Ser Leu Ser Ala Glu Lys Leu Ala Lys Val Leu Ser Thr Lys His 
705                 710                 715                 720 


Tyr Lys Gly Phe Gly Asn Val Ser Ala Lys Leu Ile Asn Gly Ile Arg 
                725                 730                 735     


Asp Lys Asn Cys Lys Thr Ile Leu Asp Tyr Leu Ile Glu Asp Asp Lys 
            740                 745                 750         


Glu Ala Tyr Tyr Gly Arg Asn Asn Pro Asn Arg Asn Leu Met Gln Leu 
        755                 760                 765             


Val Asn Asp Ser Arg Leu Ala Phe Lys Gly Gln Ile Asp Arg Glu Gln 
    770                 775                 780                 


Asn Thr His Leu Glu Asp Leu Ser Leu Asp Glu Phe Leu Asp Asp Leu 
785                 790                 795                 800 


Tyr Val Ser Pro Ser Ile Arg Arg Gly Ile Arg Leu Thr Ile Arg Leu 
                805                 810                 815     


Val Asp Glu Leu Val Glu Ile Met Gly Tyr Leu Pro Lys Asn Ile Val 
            820                 825                 830         


Ile Glu Met Pro Arg Glu Asp Gly Glu Lys Gly Lys Ile Ala Asp Thr 
        835                 840                 845             


Arg Tyr Ser Lys Leu Glu Lys Met Leu Lys Lys Asp Ala Ala Leu Glu 
    850                 855                 860                 


Asp Leu Tyr Arg Val Leu Lys Thr Tyr Glu Lys Asn Lys Lys Ala Leu 
865                 870                 875                 880 


Ala Asn Asp Ala Leu Tyr Leu Tyr Phe Leu Gln Asn Gly Arg Asp Met 
                885                 890                 895     


Tyr Thr Gly Lys Glu Ile Asn Leu Ser Glu Leu His Ser Tyr Asp Ile 
            900                 905                 910         


Asp His Ile Ile Pro Lys Ser Phe Lys Tyr Asp Asp Ser Leu Asp Asn 
        915                 920                 925             


Lys Val Leu Thr Ala Lys Lys Met Asn Met Asp Lys Arg Thr Gly Ala 
    930                 935                 940                 


Leu Asp His Asn Ile Ile Glu Asn Gln Cys Gly Phe Trp Arg Val Leu 
945                 950                 955                 960 


Leu Gln Gln Asp Lys Ile Ser Leu Glu Lys Tyr Thr Asn Leu Met Lys 
                965                 970                 975     


Thr Glu Phe Thr Glu Ala Asp Lys Ala Gly Phe Ile Met Arg Gln Leu 
            980                 985                 990         


Val Glu Thr Arg Gln Ile Thr Lys  Phe Val Ala Arg Tyr  Leu Asp Asn 
        995                 1000                 1005             


Lys Phe  Asn Gly Leu Ile Ser  Asp Pro Asn Asp Lys  Val Asn Ile 
    1010                 1015                 1020             


Leu Leu  Pro Arg Ala Ser Leu  Cys His Gln Phe Arg  Glu Thr Phe 
    1025                 1030                 1035             


Gly Phe  Tyr Lys Val Arg Glu  Leu Asn Asp Met His  His Ala His 
    1040                 1045                 1050             


Asp Ala  Tyr Leu Asn Ala Val  Ile Ala Asn Thr Leu  Asn Lys Asn 
    1055                 1060                 1065             


Ala Tyr  Leu Ser Asp Leu Leu  Lys Tyr Gly Ala Tyr  Ser Lys Tyr 
    1070                 1075                 1080             


Lys Lys  Asn Gly Phe Asn Asn  Ser Asn Gly Ile Met  Asp Tyr Phe 
    1085                 1090                 1095             


Gly Asn  Thr Gln Phe Asn Cys  Leu Phe Val Val Glu  Arg Thr Leu 
    1100                 1105                 1110             


Asp Lys  Cys Arg Val Asn Ile  Val Lys His Pro Glu  Thr Ala Ser 
    1115                 1120                 1125             


Gly Glu  Phe Tyr Asn Glu Thr  Ile Gln Lys Asn Lys  Val Asn Gly 
    1130                 1135                 1140             


Gly Ser  Ser Thr Arg Ser Leu  Lys Ser Ser Val Lys  Val Leu Gln 
    1145                 1150                 1155             


Asn Thr  Glu Gln Tyr Gly Gly  Phe Thr Asn Val Asn  Asn Ala Tyr 
    1160                 1165                 1170             


Phe Ile  Leu Phe Asp Tyr Lys  Ala Lys Ser Lys Leu  Lys Arg Lys 
    1175                 1180                 1185             


Leu Ile  Gly Val Pro Ile Val  Asp Arg Gln Lys Phe  Glu Gln Asp 
    1190                 1195                 1200             


Pro Val  Thr Tyr Leu Glu Ala  Lys Gly Phe Asp Glu  Pro Lys Leu 
    1205                 1210                 1215             


Val Gln  Lys Leu Leu Lys Tyr  Thr Leu Leu Glu Tyr  Glu Asp Gly 
    1220                 1225                 1230             


Lys Arg  Arg Tyr Leu Thr Gly  Val Thr Gly Lys Arg  Cys Glu Leu 
    1235                 1240                 1245             


Val Arg  Ala Asn Gln Leu Leu  Leu Pro Arg Asn Met  Met Ala Leu 
    1250                 1255                 1260             


Leu His  His Leu Gln Glu Trp  Gln Lys His Asp Phe  Gly Ile Lys 
    1265                 1270                 1275             


Glu Met  Thr Lys Val Ile Lys  Asn Thr Asn Asn Ile  Glu Ala Lys 
    1280                 1285                 1290             


Phe Asp  Lys Leu Phe Glu His  Met Met Lys Phe Ile  Asp Lys Tyr 
    1295                 1300                 1305             


Ser Glu  Pro Pro Lys Ile Val  Ser Ser Lys Ile Ser  Glu Glu Tyr 
    1310                 1315                 1320             


His Lys  Leu Arg Glu Ser Leu  Cys Gln Asp Asp Asn  Lys Ile Lys 
    1325                 1330                 1335             


Ile Tyr  Ala Glu Ile Gly Lys  Ala Leu Leu Ser Leu  Leu His Leu 
    1340                 1345                 1350             


Val Asp  Ser Lys Ser Ala Cys  Val Phe Lys Phe Ser  Gly Leu Glu 
    1355                 1360                 1365             


Ile Asn  Arg Ile Arg Tyr Gln  Ser Ile Asn Glu Lys  Lys Glu Pro 
    1370                 1375                 1380             


Val Ile  Ile Phe Gln Ser Leu  Ser Gly Leu Arg Glu  Ser Arg Tyr 
    1385                 1390                 1395             


Lys Tyr  Asn Gln 
    1400         


<210>  1183
<211>  1402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1183

Met Lys Leu Arg Asn Ala Lys Asp Tyr Ser Ile Gly Leu Asp Val Gly 
1               5                   10                  15      


Thr Gly Ser Thr Gly Trp Ala Val Thr Thr Leu Asp Gly Asp Leu Phe 
            20                  25                  30          


Thr Phe Asn His Arg Pro Thr Leu Gly Ser Arg Leu Phe Glu Ser Ala 
        35                  40                  45              


Ser Thr Ala Gln Glu Thr Arg Glu Ala Arg Gly Ala Arg Arg Arg Leu 
    50                  55                  60                  


Lys Arg Arg Arg Trp Arg Ile Ser Leu Leu Gln Glu Gln Phe Ala Ala 
65                  70                  75                  80  


Glu Met Glu Ser Cys Asp Ser Asn Phe Phe Ile Arg Leu Asn Gln Ser 
                85                  90                  95      


Arg Leu Trp Gln Glu Asp Arg Thr Glu Gln Gly Lys Gly Tyr Leu Phe 
            100                 105                 110         


Gly Gly Lys Pro Ile Leu Cys Gly Ala Gln Ile Phe Ala Asn Glu Gln 
        115                 120                 125             


Glu Tyr Tyr Gln Gln Phe Pro Thr Ile Phe His Leu Arg Lys Phe Leu 
    130                 135                 140                 


Val Glu Cys Ser Glu Gln Val Asp Leu Arg Leu Ile Tyr Leu Ala Leu 
145                 150                 155                 160 


His Asn Ile Val Lys Tyr Arg Gly Asn Phe Leu Tyr Gln Asp Asn Lys 
                165                 170                 175     


Thr Leu Asn Val Arg Asn Phe Asn Leu Asp Ser Gln Ile Ala Asp Phe 
            180                 185                 190         


Leu Asp Gln Leu Gln Leu Trp Cys Glu Asn Asn Asp Leu Cys Phe His 
        195                 200                 205             


Glu Ile Asp Gly Thr Thr Thr Leu Ala Ser Gln Leu Asn Thr Cys Phe 
    210                 215                 220                 


His Gln Gln Ser Asn Gln Gln Lys Lys Asp Thr Leu Lys Thr Phe Phe 
225                 230                 235                 240 


Ala Thr Cys Ile Glu Asn Asp Lys Ile Ala Gly Glu Leu Thr Lys Ala 
                245                 250                 255     


Leu Leu Gly Tyr Lys Thr Asp Phe Lys Ile Ile Phe Asn Ile Glu Gly 
            260                 265                 270         


Glu Lys Asp Glu Tyr Lys Ala Lys Cys Asn Glu Glu Glu Lys Ile Leu 
        275                 280                 285             


Lys Leu Gln Glu Thr Ile Asp Asp Ala Asn Gln Val Leu Leu Gln Ser 
    290                 295                 300                 


Ile Leu Asn Leu Tyr Ser Gly Ile Ile Val Glu Lys Ile Leu Asn Asn 
305                 310                 315                 320 


Thr Ala Val Lys Asn Glu Ser Ala Gln Cys Thr Leu Ser Ala Tyr His 
                325                 330                 335     


Cys Tyr Ser Tyr Ala Leu Tyr Ser Lys Gln Leu Arg Thr Leu Lys Lys 
            340                 345                 350         


Leu Val Arg Lys Tyr Asn Pro Gln Arg Tyr Asn Glu Cys Phe Asn Leu 
        355                 360                 365             


Ser Ala Glu Asp Pro Lys Ala Lys Lys Gln Leu Cys Thr Tyr Ala Arg 
    370                 375                 380                 


Tyr Asn Ala Gln His Ser Thr Asp Tyr Asp Glu Phe Lys Lys Tyr Ile 
385                 390                 395                 400 


Lys Asp Leu Phe Glu Asn Thr Asp Ala Val Asn Asp Ala Ala Tyr Gln 
                405                 410                 415     


Gln Met Lys Gln Asp Phe Asp Glu Glu Lys Phe Leu Arg Arg Gln Lys 
            420                 425                 430         


Thr Ser Asp Asn Gly Val Tyr Pro Tyr Gln Phe His Leu Glu Glu Met 
        435                 440                 445             


Gln Leu Ile Ile Glu Asn Gln Ala Gln His Tyr Pro Phe Leu Thr Lys 
    450                 455                 460                 


Ile Lys Pro Lys Leu Glu Ser Leu Val Thr Phe Arg Ile Pro Tyr Tyr 
465                 470                 475                 480 


Val Gly Pro Leu Thr Gln Lys Gly Ile Gln Arg Asn Gly Arg Lys Phe 
                485                 490                 495     


Ala Trp Ser Lys Arg Asn Lys Asp Ala Glu Asn Gln Arg Ile Tyr Pro 
            500                 505                 510         


Trp Asn Trp Asp Ser Ile Ile Asp Lys Ser Ala Ser Ala Glu Ala Phe 
        515                 520                 525             


Ile Leu Arg Met Thr Gly Lys Cys Thr Tyr Leu Tyr Gly Glu Asp Val 
    530                 535                 540                 


Leu Pro Lys Glu Ser Leu Leu Tyr Gln Glu Phe Cys Val Leu Asn Glu 
545                 550                 555                 560 


Leu Asn Gly Ala Arg Trp Asn Cys Asp Gly Glu Arg Arg Met Pro Leu 
                565                 570                 575     


Ser Asn Lys Asp Cys Lys Ala Ile Tyr Arg Gln Leu Phe Leu Lys Lys 
            580                 585                 590         


Gln Lys Val Ser Tyr Ala Asp Val Ser Asn Tyr Leu Lys Ala Gln Gly 
        595                 600                 605             


His Ile Asn Asn Ser Val Ala Gly Gly Gln Gly Glu His Gly Phe Glu 
    610                 615                 620                 


Ser Thr Leu Ser Ser His His Phe Phe Arg Lys Leu Phe Asn Val Asp 
625                 630                 635                 640 


Ala Ala Asn Asp Leu Thr Ala Gln Gln Ile Glu Thr Ala Glu Gln Leu 
                645                 650                 655     


Ile Leu Trp Gly Thr Ile Phe Glu Asp Lys Glu Ile Phe Glu Gln Lys 
            660                 665                 670         


Ile Lys Glu Arg Phe Gly Asp Met Phe Ser Lys Asp Glu Ile Lys Thr 
        675                 680                 685             


Ile Ala Arg Arg Lys Phe Ser Gly Trp Gly Lys Cys Ser Lys Gln Leu 
    690                 695                 700                 


Leu Ser Gly Leu Thr Val Glu Thr Pro Arg Gly Arg Glu Ser Ile Ile 
705                 710                 715                 720 


Asp Val Leu Arg Asn Gly His Pro Glu Asn Gly Arg Thr Leu Val Phe 
                725                 730                 735     


Met Gln Val Ile Thr Asp Lys Lys Leu Glu Phe Asp Lys Lys Ile Glu 
            740                 745                 750         


Glu Tyr Asn Asn Glu His Leu Ala Lys Thr Asn Glu Ser Val Leu Asp 
        755                 760                 765             


Thr Ala Ile Met Ser Pro Ala Ile Lys Arg Gly Val Asn Gln Ala Leu 
    770                 775                 780                 


His Ile Val Gly Glu Ile Arg Arg Ile Thr Lys His Asp Pro Thr Cys 
785                 790                 795                 800 


Ile Tyr Ile Glu Thr Thr Arg Asp Glu Lys Asp Gly Lys His Asn Thr 
                805                 810                 815     


Arg Thr Ser Ser Arg Tyr Ser Ser Leu Lys Lys Asn Leu Glu Thr Leu 
            820                 825                 830         


Lys Ser Ile Asp Lys Ala Asn Ala Pro Ala Ile Lys His Ser Leu Glu 
        835                 840                 845             


Leu Leu Glu Thr Tyr Lys Lys Gln Asn Ile Asp Asp Lys Leu Phe Leu 
    850                 855                 860                 


Tyr Phe Thr Gln Asn Gly Lys Cys Met Tyr Ser Gly Lys Ser Leu Asp 
865                 870                 875                 880 


Ile Asn Asn Leu Ser Ser Tyr Gln Ile Asp His Val Ile Pro Gln Ser 
                885                 890                 895     


Tyr Ile Thr Asp Asn Ser Phe Asp Asn Arg Val Leu Val Tyr Ala Ser 
            900                 905                 910         


Glu Asn Gln Arg Lys Ser Asp Ser Leu Leu Leu Ser Ser Val Ile Gln 
        915                 920                 925             


Ala Lys Gln Glu Pro Phe Trp Thr Ser Leu Glu Lys Ala Gly Leu Ile 
    930                 935                 940                 


Ser Glu Lys Lys His Asn Asn Leu Lys Arg Lys Ser Leu Ser Asp Ala 
945                 950                 955                 960 


Gln Ile Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Ile 
                965                 970                 975     


Val Lys Val Leu Arg Asp Glu Leu Ser Arg Ile Leu Pro Asp Thr Lys 
            980                 985                 990         


Ile Ile Pro Val Lys Ala Ser Leu  Ser His Asp Val Arg  Ile Ala Cys 
        995                 1000                 1005             


Asn Leu  Tyr Lys Ser Arg Thr  Ala Asn Ser Phe His  His Ala His 
    1010                 1015                 1020             


Asp Ala  Leu Leu Ala Ser Thr  Met Gly Arg Phe Val  Gln Thr Arg 
    1025                 1030                 1035             


Phe Pro  Asp Met Tyr Glu Asn  Pro Val Arg Tyr Val  Lys Ile Leu 
    1040                 1045                 1050             


Arg Ala  Tyr Ile Ala Gln Ser  Lys Leu Gln His Ser  Ala Gln Thr 
    1055                 1060                 1065             


Arg Asn  Phe Asp Ser Arg Ser  Phe Met Ala His Ser  Phe Phe Cys 
    1070                 1075                 1080             


Asp Tyr  Ser Asn Glu Asn Gly  Asp Thr Ile Trp Asn  Ser Ala Ser 
    1085                 1090                 1095             


Glu Ile  Glu Arg Leu Arg Arg  Ala Leu Ser Tyr Lys  Asn Leu Tyr 
    1100                 1105                 1110             


Leu Thr  Arg Arg Pro Phe Ile  Asp Ala Gly Gln Phe  Trp Asp Gln 
    1115                 1120                 1125             


Thr Ile  Tyr Ser Pro His Asp  Lys Lys Lys Thr Pro  Asp Ser Leu 
    1130                 1135                 1140             


Ile Pro  Leu Lys Lys Asn Leu  Asp Thr Gln Lys Tyr  Gly Gly Tyr 
    1145                 1150                 1155             


Ser Gly  Lys Asp Tyr Ala His  Phe Phe Ile Tyr Ser  Val Leu Asn 
    1160                 1165                 1170             


Lys Gln  Gly Asp Val Glu Tyr  Arg Phe Asp Gly Val  Pro Leu Tyr 
    1175                 1180                 1185             


Ile Ala  Ala Asn Ile Asn Arg  Asp Asp Arg Gly Ala  Leu Ala Gln 
    1190                 1195                 1200             


Tyr Ala  Lys Gln Leu Val Glu  His Glu Asn Val Pro  Asn Ala Lys 
    1205                 1210                 1215             


Arg Lys  Thr Lys Asp Leu Val  Phe Gln Arg Ile Glu  Arg Ala Cys 
    1220                 1225                 1230             


Val Pro  Lys Tyr Gln Leu Ile  Cys Ile Ser Gln Cys  Ser Gly Asp 
    1235                 1240                 1245             


Ser Asp  Arg Ser Val Ser Asp  Tyr Tyr Tyr Ile Thr  Gly Lys Lys 
    1250                 1255                 1260             


Glu Val  Arg Asn Ala Leu Gln  Leu Ala Phe Pro Met  His Leu Tyr 
    1265                 1270                 1275             


Gly Ala  Ile Asn Ala Ile Glu  Asp Lys Lys Glu Leu  Asp Asp Ser 
    1280                 1285                 1290             


Ala Leu  Glu Glu Leu Val Val  Tyr Ile Leu Arg Asn  Ala Pro Arg 
    1295                 1300                 1305             


Ile Ala  Pro Asp Val Phe Arg  Arg Leu Asn Leu Thr  Glu Lys Leu 
    1310                 1315                 1320             


Ala Asp  Cys Leu Asp Ile Pro  Asp Arg Ile Glu Leu  Gln Asn Ser 
    1325                 1330                 1335             


Ile Tyr  Gly Leu Phe Asn Leu  Ile Asn Ala Glu Ser  Asn Val Val 
    1340                 1345                 1350             


Asn Leu  Gln Ile Phe Gly Gly  Ala Lys Thr Ala Gly  Val Lys Leu 
    1355                 1360                 1365             


Ile Asn  Leu Asn Ser Leu Leu  Asn Asp Pro Ala Thr  Thr Val Thr 
    1370                 1375                 1380             


Phe Ile  Asp Gln Ser Val Thr  Gly Met Phe Glu Arg  Lys Glu Thr 
    1385                 1390                 1395             


Ile Gly  Ile Ser 
    1400         


<210>  1184
<211>  1402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1184

Met Gly Lys Gln Glu Phe Asn Asp Tyr Tyr Leu Gly Phe Asp Ile Gly 
1               5                   10                  15      


Thr Asn Ser Val Gly Trp Ala Val Thr Asp Gln Gln Tyr Lys Leu Leu 
            20                  25                  30          


Arg Phe Asn Lys Lys Asp Met Trp Gly Ser Arg Leu Phe Glu Glu Ala 
        35                  40                  45              


Lys Thr Ala Lys Glu Arg Arg Val Tyr Arg Asn Ser Arg Arg Arg Leu 
    50                  55                  60                  


Asp Arg Arg Lys Arg Arg Leu Lys Leu Leu Glu Glu Leu Phe Leu Asp 
65                  70                  75                  80  


Glu Ile Leu Lys Val Asp Ser Thr Phe Phe Lys Arg Leu Glu Glu Ser 
                85                  90                  95      


Asn Leu Tyr Asn Glu Asp Lys Ser Ile Lys Phe Asn Tyr Pro Leu Phe 
            100                 105                 110         


Asn Asp Lys Asp Tyr Thr Asp Ile Asp Phe Tyr Lys Glu Tyr Lys Thr 
        115                 120                 125             


Ile Tyr His Leu Arg Lys Ala Leu Val Phe Glu Asp Arg Lys Phe Asp 
    130                 135                 140                 


Ile Arg Leu Leu Phe Ile Ala Leu His His Ile Leu Lys Tyr Arg Gly 
145                 150                 155                 160 


His Phe Ile Phe Glu Gly Gln Ser Phe Glu Asp Ile Lys Ser Phe Asp 
                165                 170                 175     


Tyr Ile Phe Thr Glu Ile Gln Asp Trp Ile Leu Glu Asn Phe Glu Ile 
            180                 185                 190         


Asn Ile Asp Ile Asn Leu Lys Glu Lys Leu Glu Glu Ile Ile Leu Ser 
        195                 200                 205             


Asn Asn Thr Lys Ser Ile Lys Lys Ile Glu Phe Lys Lys Ile Phe Ser 
    210                 215                 220                 


Asp Asn Arg Ile Leu Ala Ile Leu Asn Met Gly Val Gly Cys Lys Val 
225                 230                 235                 240 


Asp Leu Lys Asp Val Phe Asn Asn Glu Glu Leu Lys Asp Gln Lys Ile 
                245                 250                 255     


Ser Phe Thr Glu Ile Asp Tyr Glu Asn Glu Lys Arg Asp Glu Phe Tyr 
            260                 265                 270         


Gln Leu Leu Gly Glu Gly Ile Phe Ile Val Asp Leu Ala Lys Lys Ile 
        275                 280                 285             


Tyr Asp Phe Met Val Leu Lys Lys Ile Leu Lys Asn Ser Asp Tyr Phe 
    290                 295                 300                 


Ser Glu Ser Lys Val Ala Leu Tyr Glu Glu His Lys Glu Asp Leu Asn 
305                 310                 315                 320 


Arg Leu Lys Tyr Ile Phe Lys Lys Tyr Leu Lys Ser Ser Asp Tyr Tyr 
                325                 330                 335     


Lys Phe Phe Arg Ser Asn Ser Glu Lys Thr Asn Tyr Pro Ser Tyr Ile 
            340                 345                 350         


Gly Leu Asn Lys Ile Lys Thr Lys Arg Leu Ile Glu Asn Asn Lys Cys 
        355                 360                 365             


Ser Ile Ser Asp Ile Asn Lys Glu Leu Lys Asn Lys Tyr Leu Lys Asp 
    370                 375                 380                 


Ile Gln Val Glu Ala Glu Asp Glu Glu Leu Phe Asn Arg Ile Leu His 
385                 390                 395                 400 


Lys Ile Asn Glu Asn Lys Phe Leu Glu Lys Leu Lys Ser Ser Glu Asn 
                405                 410                 415     


Ser Thr Val Pro Tyr Gln Val His Glu Ile Glu Leu Lys Lys Ile Leu 
            420                 425                 430         


Asp Asn Gln Arg Lys Tyr His Ser Phe Leu Asn Glu Lys Ser Asp Gly 
        435                 440                 445             


Met Thr Leu Cys Glu Lys Ile Ile Lys Leu Phe Glu Phe Arg Ile Pro 
    450                 455                 460                 


Tyr Tyr Val Gly Pro Leu Asn Thr Asn Pro Asn Met Asn Lys Lys Asn 
465                 470                 475                 480 


Lys Asn Thr Trp Val Val Lys Lys Glu Glu Asn Ser Lys Ser Ser Ile 
                485                 490                 495     


Thr Pro Trp Asn Phe Glu Lys Lys Val Asp Glu Gln Lys Ser Ala Glu 
            500                 505                 510         


Glu Phe Ile Lys Arg Met Thr Asn Arg Cys Thr Tyr Leu Arg Gly Glu 
        515                 520                 525             


Asp Val Ile Pro Lys Asn Ser Leu Leu Tyr Glu Glu Phe Val Leu Leu 
    530                 535                 540                 


Asn Glu Leu Asn Lys Val Thr Val Asp Asn Glu Leu Leu His Pro Thr 
545                 550                 555                 560 


Leu Lys Asn Glu Ile Ile Asp Glu Leu Phe Lys Thr Thr Val Lys Val 
                565                 570                 575     


Thr Lys Lys Lys Ile Thr Glu Phe Leu Lys Lys Lys Gln Ala Ile Ser 
            580                 585                 590         


Asp Val Glu Ser Ile Thr Gly Ile Asp Val Glu Phe Asn Gly Lys Leu 
        595                 600                 605             


Gln Gly Tyr Asn Thr Phe Arg Lys Ile Leu Gly Glu Lys Ile Glu Asn 
    610                 615                 620                 


Leu Asn Ile Lys Lys Phe Val Glu Arg Cys Ile Glu Leu Lys Cys Leu 
625                 630                 635                 640 


Tyr Gly Ser Asp Lys Lys Leu Phe Lys Asn Ala Val Gly Lys Leu Tyr 
                645                 650                 655     


Lys Glu Tyr Asn Val Thr Asp Glu Glu Phe Thr Lys Ile Asn Lys Leu 
            660                 665                 670         


Asn Phe Asn Lys Trp Gly Arg Leu Ser Lys Glu Phe Leu Thr Gly Ile 
        675                 680                 685             


Arg Phe Glu Lys Thr Asp Gly Thr Glu Arg Phe Glu Ser Val Ile Ala 
    690                 695                 700                 


Ala Leu Arg Ser Thr Asn Tyr Asn Leu Met Glu Leu Leu Ser Asp Lys 
705                 710                 715                 720 


Phe Asn Leu Gln Ser Lys Ile Asp Glu Ile Asn Gly Glu Asp Asp Phe 
                725                 730                 735     


Ser Asn Ile Asp Ile Lys Glu Tyr Val Asn Glu Leu Tyr Val Ser Pro 
            740                 745                 750         


Ser Val Lys Arg Ser Ile Ile Gln Thr Tyr Lys Ile Ala Met Glu Ile 
        755                 760                 765             


Lys Lys Ile Thr Gly Lys Asn Pro Lys Lys Val Phe Ile Glu Met Ala 
    770                 775                 780                 


Lys Gly Gly Gly Glu Lys Gly Lys Arg Thr Glu Arg Arg Lys Asp Lys 
785                 790                 795                 800 


Ile Lys Glu Leu Tyr Lys Asn Met Lys Asp Ser Leu Ser Asp Tyr Cys 
                805                 810                 815     


Asn Asn Leu Gln Asp Leu Ser Ser Gln Val Glu Lys Tyr Asp Asp Asn 
            820                 825                 830         


Arg Leu Arg Gln Lys Lys Leu Phe Leu Tyr Phe Met Gln Met Gly Lys 
        835                 840                 845             


Ser Met Tyr Thr Gly Asn Asp Ile Asp Leu Ser Glu Leu Phe Asn Asn 
    850                 855                 860                 


Asn Ile Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Lys Asp 
865                 870                 875                 880 


Asp Ser Phe Asp Asn Ile Val Leu Val Glu Lys Gln Glu Asn His Ser 
                885                 890                 895     


Lys Ser Asn Glu Leu Leu Lys Pro Glu Ile Gln Ser Lys Met Asn Asn 
            900                 905                 910         


Tyr Trp Ile Phe Leu Arg Glu Asn Lys Phe Ile Ser Lys Glu Lys Tyr 
        915                 920                 925             


Asn Arg Leu Thr Arg Lys Lys Asn Phe Thr Asp Glu Glu Leu Gly Asp 
    930                 935                 940                 


Phe Val Ala Arg Gln Leu Val Thr Thr Arg Gln Ser Thr Lys Glu Val 
945                 950                 955                 960 


Ala Lys Leu Phe Glu Lys Ile Phe Asp Gly Thr Glu Val Val Tyr Ser 
                965                 970                 975     


Lys Ala Ser Leu Val Ser Asp Phe Arg Glu Arg Phe Asp Ile Ile Lys 
            980                 985                 990         


Val Arg Glu Val Asn Asp Ala His  His Ala Gln Asp Ala  Tyr Leu Asn 
        995                 1000                 1005             


Ile Val  Val Gly Asn Val Phe  Asn Thr Lys Phe Thr  Lys Asn Tyr 
    1010                 1015                 1020             


Trp Lys  Phe Ile Lys Glu Asn  Asn Arg Lys Asn Glu  Glu Asn Pro 
    1025                 1030                 1035             


Asn Lys  Lys Glu Gly Tyr Asn  Phe Lys Asn Ile Tyr  Glu Ser Asp 
    1040                 1045                 1050             


Val Phe  Arg Asn Glu Asp Val  Ala Trp Val Phe Ser  Lys Glu Asn 
    1055                 1060                 1065             


Lys Glu  Ile Asn Thr Ile Lys  Thr Val Asn Lys Met  Val Tyr Arg 
    1070                 1075                 1080             


Lys Thr  Val Asn Ile Thr Glu  Met Leu Val Glu Gly  Lys Gly Gln 
    1085                 1090                 1095             


Leu Phe  Asn Leu Thr Ile Lys  Asp Arg Thr Gln Leu  Lys Lys Lys 
    1100                 1105                 1110             


Val Gly  Asn Ile Val Ala Asn  Ile Pro Lys Ser Leu  Lys Asn Arg 
    1115                 1120                 1125             


Asn Thr  Lys Leu Lys Ser Asp  Glu Leu Ala Lys Lys  Tyr Gly Tyr 
    1130                 1135                 1140             


Phe Asp  Ser Leu Asn Asn Ser  Tyr Phe Val Leu Phe  Lys Tyr Val 
    1145                 1150                 1155             


Glu Lys  Glu Lys Glu Lys Ile  Gly Phe Asp Gly Ile  Thr Ile Val 
    1160                 1165                 1170             


Asp Lys  Glu Lys Leu Lys Lys  Asn Ser Asp Ile Glu  Arg Phe Leu 
    1175                 1180                 1185             


Glu Asn  Lys Gly Asn Lys Asn  Ala Lys Leu Ile Arg  Lys Ile Phe 
    1190                 1195                 1200             


Lys Asn  Gln Lys Val Leu Ile  Asp Gly Tyr Pro Tyr  Leu Leu Lys 
    1205                 1210                 1215             


Ser Cys  Asn Lys Asp Lys Ala  Ile Arg Phe Asp Asn  Phe Lys Ser 
    1220                 1225                 1230             


Leu Tyr  Leu Asp Glu Asn Asp  Leu Lys Phe Thr Arg  Asp Ala Ile 
    1235                 1240                 1245             


Lys Phe  Glu Lys Asn Asn Ala  Asp Ile Asp Leu Lys  Lys Gln Lys 
    1250                 1255                 1260             


Tyr Ile  Ile Ser Phe Leu Lys  Lys Lys Lys Gln Asn  Glu Ile Asn 
    1265                 1270                 1275             


Glu Thr  Tyr Glu Glu Cys Ile  Glu Arg Leu Asn Asn  Glu Phe Asp 
    1280                 1285                 1290             


Thr Leu  Phe Leu Asn Leu Cys  Asp Ile Gln Lys Ser  Pro Ile Tyr 
    1295                 1300                 1305             


Lys Asn  Tyr Lys Leu Pro Asp  Phe Ile Ser Lys Lys  Asn Val Val 
    1310                 1315                 1320             


Glu Val  Lys Asp Lys Phe Glu  Val Phe Lys Asn Leu  Glu Leu Tyr 
    1325                 1330                 1335             


Asn Lys  Ser Lys Leu Ile Lys  Asn Phe Leu Asn Tyr  Phe Lys Tyr 
    1340                 1345                 1350             


Asp Asn  Asn Lys Leu Asp Leu  Ile Ala Ile Gly Ile  Asp Ser Lys 
    1355                 1360                 1365             


Ala Asn  Cys Ile Gln Lys His  Phe Thr Glu Asp Leu  Ile Lys Asp 
    1370                 1375                 1380             


Phe Val  Ile Val Asp Glu Ser  Val Thr Gly Leu Phe  Ala Lys Lys 
    1385                 1390                 1395             


Glu Arg  Leu Glu 
    1400         


<210>  1185
<211>  1402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1185

Met Thr Tyr Arg Asn Tyr His Ile Gly Leu Asp Ile Gly Thr Ser Ser 
1               5                   10                  15      


Val Gly Phe Ala Val Arg Tyr Asp Asn Gly Glu Leu Val His Lys Lys 
            20                  25                  30          


Gly Ser Asn Leu Val Gly Ala Arg Leu Phe Lys Glu Gly Glu Thr Ala 
        35                  40                  45              


Glu Glu Arg Arg Leu Asn Arg Gly Ala Arg Arg Arg Tyr Asn Arg Arg 
    50                  55                  60                  


Lys Trp Arg Leu Asn Leu Leu Asn Gln Ile Phe Lys Pro Leu Ile Glu 
65                  70                  75                  80  


Val Asn Asp Pro Thr Phe Phe Lys Arg Leu Glu Gln Ser Asn Leu Val 
                85                  90                  95      


Asn Tyr Asp Lys Ser Glu Ser Phe Ser Leu Leu Asn Asn Gln Ser Asp 
            100                 105                 110         


Asp Lys Leu His Gln Gln Phe Pro Thr Ile Tyr His Leu Arg Asp Tyr 
        115                 120                 125             


Leu Met Lys Ser Thr Glu Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala 
    130                 135                 140                 


Val His His Ile Val Lys Tyr Arg Gly Asn Phe Leu Asp Ala Ser Pro 
145                 150                 155                 160 


Val Asp Ser Phe Glu Ala Ser Asn Leu Lys Val Asp Glu Ile Leu Thr 
                165                 170                 175     


Gln Ile Asn Leu Leu Leu Asp Asn Leu Thr Asp Asp Leu Arg Leu Asn 
            180                 185                 190         


Thr Gln Tyr Val Thr Asp Ile Glu Gln Thr Leu Leu Ser Asn Thr Glu 
        195                 200                 205             


Ser Asn Lys Asp Lys Glu Lys Val Ile Gln Lys Leu Leu Leu His Lys 
    210                 215                 220                 


Glu Ala Ile Lys Ile Asp Lys Asp Arg Thr Ala Gln Ile Ala Lys Leu 
225                 230                 235                 240 


Val Leu Gly Tyr Lys Ala Asp Ile Ala Lys Leu Phe Gly Ile Thr Glu 
                245                 250                 255     


Lys Leu Gln Thr Pro Lys Leu Glu Leu Ser Ala Ala Asn Ser Asp Asp 
            260                 265                 270         


Ala Leu Met Glu Leu Ser Ala Glu Leu Asn Glu Asp Gln Ile Gly Ile 
        275                 280                 285             


Leu Leu Gln Leu Lys Thr Leu Tyr Ser Arg Val Arg Leu Asn Gln Ile 
    290                 295                 300                 


Ile Pro Ser Gly Thr Ser Leu Ser Lys Ser Met Ile Glu Lys Tyr Glu 
305                 310                 315                 320 


Leu His His Thr Gln Leu Lys Ser Phe Lys Glu Asn Ile Leu Leu Lys 
                325                 330                 335     


Leu Arg Arg Asn Thr Ala Val Arg Arg Asn Leu Glu Leu Ala Tyr Ala 
            340                 345                 350         


Leu Tyr Val Gly Asn Ala Gln Thr Lys Gln Glu Val Phe Glu Pro Lys 
        355                 360                 365             


Lys Ala Arg Phe Thr Ser Asp Asp Phe Ala Ser Leu Lys Ala Asn Ala 
    370                 375                 380                 


Lys Ser Ala Ser Ser Val Lys Glu Ala Pro Lys Asn Thr Lys Thr Lys 
385                 390                 395                 400 


Leu Thr Thr Asp Asp Phe Tyr Asn Ile Leu Thr Glu Leu Phe Ala Thr 
                405                 410                 415     


Phe Glu Ser Glu Leu Ser Ala Asp Glu Asp Met Ile Lys Glu Val Glu 
            420                 425                 430         


Gln Ile Lys Lys Ala Ile Thr Gly Asp Asn Tyr Leu Pro Lys Gln Arg 
        435                 440                 445             


Thr Asp Gln Asn Gly Asn Ile Pro His Gln Leu His Gln Ile Glu Leu 
    450                 455                 460                 


Arg Gln Ile Leu Gln Asn Gln Leu Pro His Tyr Pro Glu Met Asp Lys 
465                 470                 475                 480 


Ile Asn Glu Gln Lys Pro Ile Glu Lys Leu Val Thr Phe Arg Val Pro 
                485                 490                 495     


Tyr Tyr Ile Gly Pro Leu Ile Thr Pro Glu Asp Gln Gln Lys Ser Ser 
            500                 505                 510         


Ala Ala Asn Phe Ala Trp Met Lys Arg Lys Glu Gln Gly Ala Ile Thr 
        515                 520                 525             


Pro Trp Asn Phe Asp Asp Lys Val Asp Arg Met Gln Ser Ala Thr Asn 
    530                 535                 540                 


Phe Ile Lys Arg Met Thr Val Lys Asp Thr Tyr Leu Ile Asn Glu Asp 
545                 550                 555                 560 


Val Leu Pro Asp Arg Ser Leu Leu Tyr Gln Arg Phe Lys Val Leu Asn 
                565                 570                 575     


Glu Leu Asn Leu Val Lys Val Asn Asn Gln Leu Leu Ser Ile Gly Glu 
            580                 585                 590         


Lys Gln Ala Ile Tyr His Asp Leu Phe Glu Lys Gln Lys Thr Val Ser 
        595                 600                 605             


Thr Lys Lys Leu Gln Ser Tyr Leu Gln Ser Glu Gln Gly Tyr Gly Asp 
    610                 615                 620                 


Asn Ile Glu Ile Ser Gly Leu Ser Asn Pro Asp Lys Phe Asn Asn Gly 
625                 630                 635                 640 


Leu Thr Thr Tyr Ile Asp Phe Lys Asn Ile Phe Gly Asp Leu Val Glu 
                645                 650                 655     


Asp Thr Thr Lys Gln Lys Glu Phe Glu Gln Ile Ile Glu Trp Ser Thr 
            660                 665                 670         


Val Phe Glu Asp Arg Ala Ile Phe Lys Ala Lys Leu Gln Glu Val Asn 
        675                 680                 685             


Trp Leu Thr Glu Glu Gln Ile Lys Ala Leu Val Ala Lys Arg Tyr Ser 
    690                 695                 700                 


Gly Trp Gly Arg Leu Ser Tyr Arg Leu Leu Ala Glu Leu Val Asn Asp 
705                 710                 715                 720 


Asn Gly Glu Arg Ile Ile Asp Ile Leu Trp Asn Asn Lys Gly Ser Asn 
                725                 730                 735     


Leu Met Ser Ile Val Ser Gln Asp Glu Phe Gln Gln Gln Ile Ala Arg 
            740                 745                 750         


Ile Asn Ser His Glu Val Gln Glu Leu Gly Met Glu Ser Val Leu Ala 
        755                 760                 765             


Asn Ala Tyr Thr Ser Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Met 
    770                 775                 780                 


Lys Val Val Asp Asp Ile Gln Leu Ala Met Asn Gly Gln Ala Pro Thr 
785                 790                 795                 800 


Ser Ile Ser Ile Glu Phe Thr Arg Lys Pro Glu Glu Asn Ser Glu Ile 
                805                 810                 815     


Ser Lys Ser Arg Tyr Glu Thr Ile Thr Ser Ile Tyr Gln Ser Leu Ser 
            820                 825                 830         


Lys Asp Ile Glu Lys Glu Val Lys Asp Asn Leu Asn Lys Asn Lys Ser 
        835                 840                 845             


Ser Leu Lys Lys Asn Leu Ser Asp Lys Tyr Tyr Leu Tyr Phe Thr Gln 
    850                 855                 860                 


Leu Gly Arg Asp Ile Tyr Thr Asn Gln Pro Ile Pro Phe Asn Glu Ile 
865                 870                 875                 880 


Pro Tyr Tyr Asp Ile Asp His Ile Val Pro Arg Ser Val Glu Met Asn 
                885                 890                 895     


Asp Ser Leu Asn Asn Arg Val Leu Val Ala Lys Ser Ile Asn Gln Ile 
            900                 905                 910         


Lys Lys Asn Asn Ala Pro Ile Asp Ser Ser Leu Lys Ile Thr Ser Glu 
        915                 920                 925             


Thr Arg Gln Leu Trp Lys Lys Leu Leu Asp Ser Gly Leu Ile Thr Lys 
    930                 935                 940                 


Gln Lys Tyr Tyr Lys Leu Ser Ser Ser Ile Lys Asn Thr Val Ser Pro 
945                 950                 955                 960 


Tyr Val Lys Gln Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Ser Gln 
                965                 970                 975     


Val Ile Lys Leu Val Ala Asn Ile Leu Gly Thr Lys Tyr Gln Asn Asp 
            980                 985                 990         


Asp Thr Glu Ile Ile Glu Val Lys  Ala His Leu Asn Ser  Glu Ile Arg 
        995                 1000                 1005             


Lys Thr  Phe Glu Phe Tyr Lys  Leu Arg Glu Leu Asn  Asp Tyr His 
    1010                 1015                 1020             


His Gly  Phe Asp Ala Tyr Leu  Thr Thr Leu Val Gly  Gly Tyr Leu 
    1025                 1030                 1035             


Tyr Asn  Arg Tyr Pro Lys Leu  Arg Ser Tyr Phe Val  Tyr Gly Asp 
    1040                 1045                 1050             


His Lys  Arg Leu Asp Lys Ala  Gln Leu Ser Glu Leu  Lys Asn Phe 
    1055                 1060                 1065             


Asn Phe  Leu His Gly Leu Thr  Asn Pro Lys Ser Gly  Ser Asp Gly 
    1070                 1075                 1080             


Leu Ile  Thr Ala Ser Gly Ser  Gln Gln Val Val Leu  Asp Arg Asn 
    1085                 1090                 1095             


Glu Thr  Ile Lys Arg Leu Asn  Lys Ile Tyr Arg Tyr  Lys Tyr Met 
    1100                 1105                 1110             


Leu Ile  Ser Arg Glu Val Phe  Thr Lys Gln Gly Gln  Phe Tyr Asn 
    1115                 1120                 1125             


Glu Thr  Ile Tyr Pro Ala Lys  Pro Asn Thr Ala Lys  Met Ile Pro 
    1130                 1135                 1140             


Leu Lys  Asp Asn Lys Pro Ser  Glu Val Tyr Gly Gly  Tyr Ser Ser 
    1145                 1150                 1155             


Ser Asn  Val Ser Lys Ile Ala  Leu Ile Lys Glu Lys  Asn Thr Asp 
    1160                 1165                 1170             


Asn Tyr  Gln Phe Ile Gly Ile  Pro Ile Arg Ser Ile  Ala Lys Leu 
    1175                 1180                 1185             


Lys Gln  Ala Lys Ala Asn Ser  Lys Thr Val Tyr Gln  Thr Glu Leu 
    1190                 1195                 1200             


Asn Arg  Val Ile Lys Ser Val  Leu Ala Ser Gln Lys  Thr Lys Lys 
    1205                 1210                 1215             


Gln Lys  Glu Phe Glu Val Ile  Leu Asp Asn Val Lys  Tyr Asn Gln 
    1220                 1225                 1230             


Val Val  Val Asp Gln Gly Glu  Lys Tyr Tyr Leu Gly  Ser Pro Tyr 
    1235                 1240                 1245             


Tyr Lys  Lys Asn Ala Lys Gln  Leu Val Ile Ser Pro  Glu Ser Val 
    1250                 1255                 1260             


Lys Ile  Leu Ala Asp Lys Ala  Thr Ala Asp Leu Pro  Glu Gln Glu 
    1265                 1270                 1275             


Ala His  Asn Lys Leu Met Thr  Val Tyr Gln Glu Ile  Val Asp Val 
    1280                 1285                 1290             


Leu Asn  Lys His Phe Ala Leu  Tyr Asp Ile Asn Asn  Val Arg Lys 
    1295                 1300                 1305             


Lys Leu  Asn Asp Gly Leu Ile  Lys Phe Ser Gln Leu  Pro Asn Tyr 
    1310                 1315                 1320             


Asp Glu  Thr Ser Lys Asn Arg  Phe Asp Ile Ser Lys  Ser Ile Lys 
    1325                 1330                 1335             


Thr Lys  Tyr Ala Val Ile Met  Arg Ile Leu Ser Gly  Ile Lys Asn 
    1340                 1345                 1350             


Gln Thr  Ser Glu Val Pro Glu  Leu Gly Leu Lys Ser  Pro Leu Asn 
    1355                 1360                 1365             


Lys Met  Gln Thr Lys Gly Gly  Ile Lys Ile Ser Pro  Glu Ala Lys 
    1370                 1375                 1380             


Ile Tyr  Tyr Ser Ser Pro Thr  Gly Ile Phe Ser His  Phe Lys Gln 
    1385                 1390                 1395             


Val Lys  Asn Leu 
    1400         


<210>  1186
<211>  1402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1186

Met Glu Arg Ile Leu Gly Leu Asp Leu Gly Val Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Glu Glu Asp Val Lys Asn Pro Glu Asn Asn Lys Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Ile Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Gly Arg Thr Ser Ala 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Phe Lys Leu Arg Arg Lys Lys 
65                  70                  75                  80  


Leu Leu Glu Ile Leu Ile His Tyr Lys Ile Ile Lys Asn Asp Ala Val 
                85                  90                  95      


Leu Ala Glu Thr Gly Lys Asn Thr Thr Phe Gln Thr Gln Tyr Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Arg Glu Lys Ile Glu Leu Asp Glu Leu Ala Arg Val 
        115                 120                 125             


Leu Leu Leu Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Val 
    130                 135                 140                 


Gln Asn Asp Asp Asp Gly Lys Ala Ile Asp Gly Leu Ser Val Ala Lys 
145                 150                 155                 160 


Glu Leu Tyr Glu Lys Asn Tyr Thr Pro Gly Gln Tyr Ala Tyr Asp Leu 
                165                 170                 175     


Leu Lys Arg Gly Lys Lys Gln Ile Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Phe Gln Lys Gln Tyr His Pro 
        195                 200                 205             


Asp Ile Leu Thr Asp Glu Phe Lys Lys Glu Leu Glu Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ala Ala Ile Phe Leu Asn Arg Tyr Lys Ile Tyr Thr Ala 
225                 230                 235                 240 


Asp Asn Lys Gly Thr Arg Glu Glu Lys Lys Ile Gln Ala Tyr Gln Trp 
                245                 250                 255     


Arg Val Glu Ala Leu Ser Gln Pro Leu Asp Val Lys Glu Val Ala Tyr 
            260                 265                 270         


Val Ile Ala Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Lys Leu Tyr Phe Asn Gly Gln Thr 
    290                 295                 300                 


Ile Gly Glu Tyr Leu Phe Glu Glu Leu Ser Ala Asn Pro His Thr Arg 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Gln Asp Glu Phe Glu 
                325                 330                 335     


Lys Ile Trp Lys Ile Gln Thr Lys Phe His Pro Glu Leu Thr Lys Glu 
            340                 345                 350         


Leu Lys Glu Gln Val Arg Asp Ile Val Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Arg Glu 
    370                 375                 380                 


Ile Glu Ile Glu Glu Asn Gly Lys Arg Lys Arg Lys Lys Val Gly Leu 
385                 390                 395                 400 


Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp 
                405                 410                 415     


Gln Val Leu Asn Asn Ile Glu Ile Lys Asn Glu Asp Glu Arg Arg Tyr 
            420                 425                 430         


Leu Glu Pro Glu Glu Lys Glu Leu Leu Phe Asn Glu Leu Asn Cys Lys 
        435                 440                 445             


Gly Asn Leu Thr Ser Gln Lys Cys Leu Glu Leu Leu Gly Phe Lys Ser 
    450                 455                 460                 


Lys Asp Ala Lys Leu Asn Phe Lys Val Val Glu Gly Asn Arg Thr Asn 
465                 470                 475                 480 


Glu Lys Leu Tyr Asp Ala Phe Leu Lys Ile Leu Glu Leu Gln Gly Tyr 
                485                 490                 495     


Asn Val Phe Glu Leu Leu Lys Ile Lys Glu Asp Lys Asp Glu Ala Lys 
            500                 505                 510         


Leu Ser Glu Leu Lys Ala Ser Ala Asp Glu Ile Lys Gly Met Ile Lys 
        515                 520                 525             


Glu Ile Phe Asn Ala Asn Leu Ile Asn Thr Ser Ile Leu Asp Phe Asn 
    530                 535                 540                 


Ala Glu Leu Glu Gly Lys Asp Phe Glu Asn Gln Ala Ser Tyr Gln Phe 
545                 550                 555                 560 


Trp His Leu Met Tyr Ser Tyr Glu Gly Asp Asn Ser Pro Ser Gly Asn 
                565                 570                 575     


Glu Lys Leu Tyr Glu Leu Leu Glu Lys Lys Phe Gly Phe Lys Lys Glu 
            580                 585                 590         


His Ser Lys Ile Leu Ser Thr Ile Val Phe Ser Gln Glu Tyr Gly Ser 
        595                 600                 605             


Leu Ser Thr Lys Ala Met Arg Arg Ile Tyr Pro Tyr Ile Lys Asp Asn 
    610                 615                 620                 


Lys Tyr Ser Glu Ala Cys Leu Leu Ala Gly Tyr Asn His Ser Lys His 
625                 630                 635                 640 


Ser Leu Thr Lys Glu Glu Leu Glu Asn Arg Lys Leu Lys Glu Gln Leu 
                645                 650                 655     


Glu Val Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile 
            660                 665                 670         


Leu Asn Gln Met Ile Asn Leu Val Asn Ala Thr Ile Ser Glu Tyr Gly 
        675                 680                 685             


Lys Leu Asp Gly Val Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Ser 
    690                 695                 700                 


Ala Gln Glu Arg Glu Asp Met Ala Lys Ser Ile Asn Glu Ala Thr Leu 
705                 710                 715                 720 


Leu His Gln Lys Tyr Ala Thr Ile Leu Lys Gln Glu Phe Gly Val Val 
                725                 730                 735     


Asn Pro Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Met Glu Leu 
            740                 745                 750         


Thr Val Asn Gly Tyr Lys Asp Leu Tyr Thr Asn Thr Lys Ile Glu Lys 
        755                 760                 765             


Glu Asn Leu Phe Thr Asp Lys Tyr Asp Ile Asp His Ile Ile Pro Gln 
    770                 775                 780                 


Ser Arg Phe Phe Asp Asp Ser Phe Ser Asn Lys Val Leu Val Pro Arg 
785                 790                 795                 800 


Gln Ser Asn Leu Asp Lys Gly Asn Leu Thr Ala Tyr Asp Phe Met Ser 
                805                 810                 815     


Asn Lys Gly Ser Glu Arg Glu Glu His Phe Leu Asn Val Ile Lys Glu 
            820                 825                 830         


Leu Leu Asp Lys Gly Ser Ile Ser Lys Ala Lys Tyr Glu Lys Leu Lys 
        835                 840                 845             


Lys Lys Gly Leu Glu Ile Gly Asp Gly Phe Ile Glu Arg Asp Leu Arg 
    850                 855                 860                 


Asp Thr Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Phe Glu Ile 
865                 870                 875                 880 


Thr Asn Ser Val Val Thr Thr Ser Gly Arg Ile Thr Asp Lys Leu Arg 
                885                 890                 895     


Lys Asp Trp Asp Leu Val Asn Ile Met Lys Glu Leu Asn Leu Asp Asn 
            900                 905                 910         


Tyr Arg Arg Leu Gly Leu Thr Glu Ile Val Arg Asn Ser Lys Gly Glu 
        915                 920                 925             


Glu Lys Glu Leu Ile Pro Asp Trp Thr Lys Arg Asn Asp His Arg His 
    930                 935                 940                 


His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Thr Arg Asn His Ile 
945                 950                 955                 960 


Gln Tyr Leu Asn Tyr Leu Asn Ala Arg Lys Asp Glu Lys His Lys Glu 
                965                 970                 975     


His Gln Asn Ile Tyr Ala Ile Glu Asn Ile Ile Thr Glu Val Ile Glu 
            980                 985                 990         


Lys Lys Asn Gly Ser Lys Thr Arg  Arg Phe Lys Ala Pro  Met Asn Ser 
        995                 1000                 1005             


Phe Arg  His Glu Ala Lys Gln  His Leu Lys Glu Ile  Leu Val Ser 
    1010                 1015                 1020             


His Lys  Thr Lys Asn Lys Val  Val Thr Ser Asn Ile  Asn Lys Thr 
    1025                 1030                 1035             


Lys Lys  Lys Gly Gly Ile His  Lys Lys Thr Glu Leu  Thr Pro Arg 
    1040                 1045                 1050             


Gly Gln  Leu His Lys Glu Thr  Val Tyr Gly Ser Lys  Lys Phe Leu 
    1055                 1060                 1065             


Gln Ser  Arg Glu Glu Lys Val  Ser Ala Lys Phe Asp  Tyr Glu Thr 
    1070                 1075                 1080             


Ile Leu  Met Val Val Asn Pro  Ile His Arg Asn Ala  Leu Leu Asn 
    1085                 1090                 1095             


Arg Leu  Lys Glu Tyr Gly Asn  Asp Pro Lys Lys Ala  Phe Ser Gly 
    1100                 1105                 1110             


Lys Asn  Ala Ile Asn Lys Asn  Pro Val Tyr Leu Asp  Asp Ser Lys 
    1115                 1120                 1125             


Thr Asp  Ile Leu Ser Glu Lys  Val Thr Leu Ser Trp  Tyr Glu Thr 
    1130                 1135                 1140             


Gly Tyr  Thr Ile Arg Lys Ser  Val Thr Pro Asp Asn  Phe Lys Asp 
    1145                 1150                 1155             


Tyr Lys  Asn Leu Glu Lys Ile  Thr Asp Leu Gly Ile  Lys Lys Ile 
    1160                 1165                 1170             


Leu Lys  Glu Arg Leu Asp Gln  Phe Lys Gly Asn Ala  Lys Glu Ala 
    1175                 1180                 1185             


Phe Ser  Asn Leu Asp Lys Asp  Pro Ile Trp Leu Asn  Glu Glu Lys 
    1190                 1195                 1200             


Gly Ile  Ala Ile Lys Thr Val  Thr Ile Thr Gly Val  Ser Asn Ala 
    1205                 1210                 1215             


Glu Ser  Leu His Phe Lys Lys  Asp His Leu Gly Lys  Glu Ile Leu 
    1220                 1225                 1230             


Asp Glu  Asn Gly Asn Lys Ile  Pro Gly Asp Phe Val  Ser Thr Gly 
    1235                 1240                 1245             


Asn Asn  His His Leu Ala Ile  Tyr Leu Asp Glu Glu  Glu Lys Leu 
    1250                 1255                 1260             


Asp Asp  Lys Met Val Thr Phe  Tyr Glu Ala Val Leu  Arg Val Asn 
    1265                 1270                 1275             


Gln Gly  Leu Pro Val Ile Asp  Lys Asp Tyr Asn Lys  Glu Lys Gly 
    1280                 1285                 1290             


Tyr Lys  Phe Leu Met Thr Leu  Lys Gln Asn Glu Met  Phe Val Phe 
    1295                 1300                 1305             


Pro Asn  Glu Asp Phe Asp Pro  Asn Glu Ile Asp Leu  Leu Asp Glu 
    1310                 1315                 1320             


Arg Asn  Leu Glu Arg Ile Ser  Lys His Leu Phe Arg  Val Gln Lys 
    1325                 1330                 1335             


Ile Ser  Lys Val Gly Tyr Gly  Asn Ser Phe Val Arg  Asp Phe Val 
    1340                 1345                 1350             


Phe Arg  His His Leu Glu Thr  Thr Val Glu Glu Arg  Lys Glu Leu 
    1355                 1360                 1365             


Arg Asn  Ile Thr Tyr Ile Gln  Leu Lys Ser Leu Glu  Gly Leu Arg 
    1370                 1375                 1380             


Asn Ile  Ile Lys Val Arg Leu  Asn His Leu Gly Lys  Ile Val Gln 
    1385                 1390                 1395             


Ile Gly  Glu Tyr 
    1400         


<210>  1187
<211>  1404
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1187

Met Thr Lys Asn Ile Leu Gly Leu Asp Leu Gly Val Ser Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Val Gln Glu Asp Ser Lys Asn Pro Glu Asn Asn Arg Ile 
            20                  25                  30          


Val Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Gln Ile 
        35                  40                  45              


Asn Phe Glu Lys Gly Lys Pro Ile Thr Thr Asn Ala Ser Arg Thr Leu 
    50                  55                  60                  


Ala Arg Ser Ala Arg Arg Asn Leu Gln Arg Phe Lys Phe Arg Arg Ala 
65                  70                  75                  80  


Asn Leu Ile Asp Val Leu Lys Lys Cys Asn Ile Leu Lys Gln Gly Asp 
                85                  90                  95      


Leu Leu Thr Glu Ile Gly Lys Asn Ser Thr Phe Gln Thr Gln Glu Leu 
            100                 105                 110         


Arg Ala Lys Ser Ala Lys Glu Lys Val Glu Leu Ser Asp Phe Val Arg 
        115                 120                 125             


Val Leu Leu Leu Met Asn Lys Lys Arg Gly Tyr Arg Ser Ser Arg Lys 
    130                 135                 140                 


Ala Lys Asn Glu Asp Glu Gly Gln Ile Ile Asp Gly Met Ser Val Ala 
145                 150                 155                 160 


Lys Lys Leu Tyr Glu Glu Asn Leu Thr Pro Gly Glu Tyr Ser Tyr Gln 
                165                 170                 175     


Leu Leu Ile Glu Asn Lys Lys Gln Leu Pro Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         


Leu Gln Ser Glu Phe Asp Arg Val Trp Lys Phe Gln Lys Gln Phe Asn 
        195                 200                 205             


Pro Glu Ile Leu Thr Asp Asp Leu Tyr Lys Glu Leu Gln Gly Lys Asn 
    210                 215                 220                 


Lys Asn Ala Thr Trp Ala Val Leu Lys Glu Pro Phe Ser Leu Val Gly 
225                 230                 235                 240 


Ile Lys Gln Ile Gly Thr Ile Gln Glu Lys Lys Ala Glu Lys Tyr Phe 
                245                 250                 255     


Trp Arg Ser Glu Ala Val Lys Arg Gln Leu Asp Phe Glu Ser Leu Ala 
            260                 265                 270         


Val Val Phe Gln Glu Ile Asn Ser Asn Leu Asn Asn Ser Ser Gly Tyr 
        275                 280                 285             


Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Gln 
    290                 295                 300                 


Thr Val Gly Glu Tyr Leu Phe Gln Gln Leu Lys Glu Asn Ser His Thr 
305                 310                 315                 320 


Lys Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe 
                325                 330                 335     


Glu Lys Ile Trp Glu Thr Gln Ser Lys Tyr Tyr Lys Glu Leu Thr Lys 
            340                 345                 350         


Glu Leu Lys Glu Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Gly Leu Ile Ser Ile Cys Glu Phe Glu Asn Arg 
    370                 375                 380                 


Glu Ile Glu Ile Thr Glu Asn Gly Lys Ile Lys Lys Lys Thr Val Gly 
385                 390                 395                 400 


Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile 
                405                 410                 415     


Trp Gln Val Leu Asn Asn Leu Val Phe Gln Ser Ile Glu Ser Lys Glu 
            420                 425                 430         


Leu Phe Pro Leu Asp Leu Asp Ile Lys Glu Ser Ile Phe Asn Glu Val 
        435                 440                 445             


Asn Ile Lys Gly Asn Leu Ser Ala Lys Asn Val Leu Asp Leu Met Gly 
    450                 455                 460                 


Tyr Ser Gly Lys Glu Trp Lys Thr Asn Phe Lys Asn Ile Glu Gly Asn 
465                 470                 475                 480 


Arg Thr Asn Glu Asn Leu Tyr Thr Ala Phe Leu Lys Ile Ile Ala Ser 
                485                 490                 495     


Glu Gly Ile Glu Phe Pro Lys Glu Phe Lys Leu Ser Ile Asp Asp Asp 
            500                 505                 510         


Ile Lys Val Ser Lys Ile Asp Ile Ser Ser Glu Lys Ile Lys Glu Phe 
        515                 520                 525             


Ile Lys Glu Asn Phe Leu Lys Leu Gly Ile Asn Thr Ser Ile Leu Asp 
    530                 535                 540                 


Phe Asn Pro Glu Leu Asp Gly Lys Asp Phe Glu Lys Gln Glu Ser Tyr 
545                 550                 555                 560 


Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp Ser Ala Ser 
                565                 570                 575     


Gly Asn Val Lys Leu Tyr Glu Leu Leu Glu Lys Lys Phe Gly Phe Lys 
            580                 585                 590         


Lys Glu His Ser Lys Ile Leu Ala Asp Val Gly Phe Ser Pro Asp Tyr 
        595                 600                 605             


Gly Ser Leu Ser Ser Lys Ala Met Arg Lys Ile Tyr Pro Tyr Ile Lys 
    610                 615                 620                 


Glu Asn Lys Tyr Ser Asp Ala Cys His Leu Ala Gly Tyr Asn His Ser 
625                 630                 635                 640 


Lys Asn Ser Phe Thr Lys Asp Gln Leu Ala Asn Arg Ile Leu Lys Glu 
                645                 650                 655     


Gln Leu Glu Ile Leu Pro Lys Asn Ala Leu Arg Asn Pro Val Val Glu 
            660                 665                 670         


Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Glu Val Ile Lys Ala 
        675                 680                 685             


Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys 
    690                 695                 700                 


Lys Asn Ala Glu Glu Arg Ala Asn Met Thr Ser Asp Ile Ser Lys Ala 
705                 710                 715                 720 


Thr Leu Leu His Gln Lys Tyr Ala Glu Val Leu Gln Lys Glu Phe Gly 
                725                 730                 735     


Ile Lys Ala Pro Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Leu 
            740                 745                 750         


Glu Leu Glu Lys Asn Leu Phe Lys Asp Leu Tyr Thr Asp Val Gln Ile 
        755                 760                 765             


Lys Arg Glu Glu Leu Phe Thr Asp Lys Tyr Asp Ile Asp His Ile Ile 
    770                 775                 780                 


Pro Gln Ser Arg Phe Phe Asp Asp Ser Phe Ser Asn Lys Val Leu Val 
785                 790                 795                 800 


Pro Arg Gly Ala Asn Leu Lys Lys Gly Asn Phe Thr Ala Leu Asp Tyr 
                805                 810                 815     


Met Glu Ile Glu Gly Lys Glu Lys Leu Glu Lys Phe Leu Asn Ile Ile 
            820                 825                 830         


Lys Glu Leu Phe Asp Lys Gly Ala Ile Ser Lys Ala Lys Phe Glu Lys 
        835                 840                 845             


Leu Gln Lys Lys Gly Ser Glu Ile Gly Glu Gly Phe Ile Glu Arg Asp 
    850                 855                 860                 


Leu Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Phe 
865                 870                 875                 880 


Gly Ile Thr Asn Ser Val Ile Ser Thr Ser Gly Arg Ile Thr Asp Lys 
                885                 890                 895     


Leu Arg Glu Asp Trp Asn Leu Val Asn Thr Met Lys Glu Leu Asn Leu 
            900                 905                 910         


Glu Lys Tyr Arg Lys Leu Gly Leu Thr Glu Leu Ile Val Asn Ser Lys 
        915                 920                 925             


Gly Glu Glu Lys Glu Arg Ile Ile Asp Trp Thr Lys Arg Asn Asp His 
    930                 935                 940                 


Arg His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Thr His Asn 
945                 950                 955                 960 


His Ile Gln Tyr Leu Asn Tyr Leu Asn Ala Arg Lys Asp Glu Lys His 
                965                 970                 975     


Lys Glu His Gln Asn Ile Phe Ala Ile Glu Asn Thr Ile Thr Glu Ile 
            980                 985                 990         


Val Glu Lys Lys Asn Gly Ser Lys  Glu Arg Arg Phe Lys  Leu Pro Ile 
        995                 1000                 1005             


Lys Asn  Phe Arg Thr Glu Ala  Lys Lys Gln Leu Glu  Glu Val Leu 
    1010                 1015                 1020             


Ile Ser  His Lys Ala Lys Asn  Lys Val Val Thr Lys  Asn Ile Asn 
    1025                 1030                 1035             


Lys Ile  Lys Lys Lys Gly Ser  Ile Ile Ala Lys Thr  Glu Leu Thr 
    1040                 1045                 1050             


Pro Arg  Gly Gln Leu His Lys  Glu Thr Ile Tyr Gly  Ser Ser Lys 
    1055                 1060                 1065             


Phe Leu  Lys Thr Lys Glu Glu  Lys Ile Ser Gly Lys  Phe Asp Leu 
    1070                 1075                 1080             


Glu Met  Ile Gln Lys Val Gln  Asn Glu Lys Tyr Arg  Asn Ala Leu 
    1085                 1090                 1095             


Leu Lys  Arg Leu Gln Glu Phe  Gly Gly Asp Ser Lys  Lys Ala Phe 
    1100                 1105                 1110             


Thr Gly  Lys Asn Val Ile Ser  Lys Asn Pro Ile Phe  Leu Asn Ala 
    1115                 1120                 1125             


Glu Lys  Thr Glu Gln Leu Pro  Glu Thr Val Thr Leu  Ala Trp Tyr 
    1130                 1135                 1140             


Glu Thr  Gly Tyr Thr Ile Arg  Lys Val Val Asn Pro  Asp Asn Phe 
    1145                 1150                 1155             


Lys Asp  Phe Lys Asn Ile Glu  Lys Val Ile Asp Lys  Gly Val Arg 
    1160                 1165                 1170             


Asp Ile  Leu Thr Glu Arg Leu  Lys Glu Phe Asn Gly  Asn Ser Lys 
    1175                 1180                 1185             


Glu Ala  Phe Ser Asn Leu Glu  Lys Asn Pro Ile Trp  Leu Asn Lys 
    1190                 1195                 1200             


Ser Lys  Gly Ile Ser Ile Lys  Thr Val Thr Ile Thr  Gly Ile Asn 
    1205                 1210                 1215             


Asn Ala  Glu Ala Leu His Tyr  Lys Lys Asp His Leu  Gly Lys Glu 
    1220                 1225                 1230             


Ile Leu  Asp Glu Asn Gly Gln  Arg Ile Ala Val Asp  Phe Val Ser 
    1235                 1240                 1245             


Thr Gly  Asn Asn His His Val  Ala Ile Tyr Glu Asp  Asp Asn Gly 
    1250                 1255                 1260             


Asn Leu  Gln Glu Lys Val Val  Ser Phe Tyr Glu Ala  Val Glu Arg 
    1265                 1270                 1275             


Val Asn  Gln Asn Leu Pro Ile  Ile Asn Lys Glu Tyr  Asn Phe Glu 
    1280                 1285                 1290             


Leu Gly  Trp Lys Phe Leu Phe  Thr Met Lys Gln Asn  Glu Met Phe 
    1295                 1300                 1305             


Leu Phe  Pro Ser Asp Asp Phe  Asp Pro Lys Glu Val  Asp Leu Phe 
    1310                 1315                 1320             


Asp Asp  Lys Tyr Leu Ser Leu  Ile Ser Lys Asn Leu  Phe Arg Val 
    1325                 1330                 1335             


Gln Ser  Ile Ser Ile Val Gln  Tyr Gly Asn Ser Val  Ile Arg Asp 
    1340                 1345                 1350             


Phe Lys  Phe Arg His His Leu  Glu Thr Ser Val Ser  Asp Glu Lys 
    1355                 1360                 1365             


Lys Leu  Gln Gly Val Thr Tyr  Gln Gln Ile Lys Ser  Leu Gln Pro 
    1370                 1375                 1380             


Leu Lys  Asp Ile Ile Lys Ile  Arg Leu Asn Asn Leu  Gly Lys Ile 
    1385                 1390                 1395             


Val Gln  Ile Gly Glu Tyr 
    1400                 


<210>  1188
<211>  1404
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1188

Met Lys Gly Asn Ile Glu Lys Lys Lys Gln Glu Phe Asn Asp Tyr Tyr 
1               5                   10                  15      


Leu Gly Phe Asp Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ser Asp 
            20                  25                  30          


Leu Ser Tyr Asn Leu Leu Arg Phe Asn Lys Lys Asp Met Trp Gly Ala 
        35                  40                  45              


Arg Leu Phe Asp Glu Ala Lys Thr Ala Lys Asp Arg Arg Val Lys Arg 
    50                  55                  60                  


Asn Ser Arg Arg Arg Leu Glu Arg Arg Ser Lys Arg Leu Lys Tyr Leu 
65                  70                  75                  80  


Glu Glu Ile Phe Glu Thr Glu Ile Cys Lys Ile Asp Lys Asn Phe Phe 
                85                  90                  95      


Arg Arg Leu Glu Glu Ser Thr Leu His Asn Glu Asp Lys Thr Phe Asp 
            100                 105                 110         


Phe Arg Phe Pro Ile Phe Asn Asp Glu Asn Tyr Thr Asp Lys Glu Tyr 
        115                 120                 125             


Tyr Lys Asp Phe Pro Thr Val Tyr His Leu Arg Asn Glu Leu Ile Lys 
    130                 135                 140                 


Asn Glu Asn Lys Lys Phe Asp Ile Arg Leu Ile Tyr Leu Ala Ile His 
145                 150                 155                 160 


Asn Ile Leu Lys Tyr Arg Gly His Phe Ile Phe Glu Gly Gln Asp Phe 
                165                 170                 175     


Asp Ser Val Thr Asp Phe Lys Glu Val Phe Ser Glu Leu Ser Ala Tyr 
            180                 185                 190         


Leu Ser Glu Asn Leu Glu Glu Asn Leu Asp Ile Asn Leu Cys Asp Asn 
        195                 200                 205             


Ile Lys Asp Ile Ile Cys Ser Lys Glu Asn Lys Thr Ser Lys Gly Lys 
    210                 215                 220                 


Ser Phe Val Ser Leu Ile Asp Ser Lys Val Ile Gln Ser Val Leu Arg 
225                 230                 235                 240 


Cys Cys Ile Gly Tyr Arg Val Lys Phe Lys Asp Ile Phe Ile Asn Tyr 
                245                 250                 255     


Glu Glu Lys Glu Lys Asn Glu Gly Ile Asn Leu Ser Glu Ile Glu Tyr 
            260                 265                 270         


Asp Glu Lys Arg Asp Glu Ile Ala Leu Ser Leu Gly Glu Asn Ile Leu 
        275                 280                 285             


Leu Ile Asp Ile Cys Lys Lys Val Tyr Asp Phe Met Ile Leu Asn Asn 
    290                 295                 300                 


Ile Leu Lys Gly Asn Lys Tyr Ile Ser Glu Ser Lys Val Asn Leu Tyr 
305                 310                 315                 320 


Asp Glu His Lys Cys Asp Leu Cys Asn Leu Lys Tyr Ile Phe Lys Lys 
                325                 330                 335     


Tyr Leu Pro Lys Lys Asp Tyr Phe Glu Phe Phe Arg Asn Glu Asn Lys 
            340                 345                 350         


Glu Lys Asn Tyr Val Ser Tyr Ile Ser Ser Ser Leu Asn Asn Gly Lys 
        355                 360                 365             


Lys Lys Asn Thr Cys Thr Gln Glu Asp Leu Asn Lys Glu Ile Lys Glu 
    370                 375                 380                 


Tyr Ile Ala Lys Ile Lys Glu Tyr Glu Glu Asn Asp Ala Val Leu Glu 
385                 390                 395                 400 


Lys Ile Asn Leu Lys Leu Asn Glu Asn Lys Leu Leu Pro Lys Leu Lys 
                405                 410                 415     


Thr Asn Asp Asn Gly Val Ile Pro Tyr Gln Ile His Lys Ile Glu Leu 
            420                 425                 430         


Glu Lys Ile Leu Gln Asn Gln Ser Lys Tyr Tyr Asp Phe Leu Leu Glu 
        435                 440                 445             


Lys Ile Asp Gly Ile Thr Asn Ile Glu Arg Ile Glu Lys Leu Phe Thr 
    450                 455                 460                 


Phe Arg Ile Pro Tyr Tyr Ile Gly Thr Leu Lys Gly Lys Phe Ser Trp 
465                 470                 475                 480 


Ala Lys Leu Lys Glu Asn Lys Lys Ile Thr Pro Trp Asn Phe Glu Lys 
                485                 490                 495     


Ile Val Asp Met Glu Glu Ser Ala Lys Ala Phe Ile Glu Arg Met Ser 
            500                 505                 510         


Asn Arg Cys Ser Tyr Leu Lys Thr Glu Asn Val Leu Pro Lys Asn Ser 
        515                 520                 525             


Leu Leu Tyr Ser Glu Tyr Glu Leu Leu Asn Glu Leu Asn Lys Val Lys 
    530                 535                 540                 


Ile Lys Asn Glu Phe Ile Asn Val Val Leu Lys Asn Glu Ile Ile Asp 
545                 550                 555                 560 


Glu Leu Phe Lys Thr Thr Ser Lys Val Thr Lys Lys Lys Phe Val Asp 
                565                 570                 575     


Tyr Leu Lys Thr Lys Gln Ile Phe Val Glu Lys Glu Asp Ile Ser Gly 
            580                 585                 590         


Leu Asp Asn Glu Phe Lys Ser Lys Leu Asp Ser Tyr Ile Lys Phe Lys 
        595                 600                 605             


Lys Val Ile Gly Asp Lys Ile Asp Thr Leu Arg Gly Tyr Asn Phe Val 
    610                 615                 620                 


Glu Lys Cys Ile Glu Phe Lys Cys Phe Tyr Gly Asp Asp Lys Lys Leu 
625                 630                 635                 640 


Phe Lys Asn Ala Ile Asn Asn Thr Glu Phe Lys Asp Tyr Val Ser Asp 
                645                 650                 655     


Asp Glu Leu Lys Lys Ile Asn Lys Met Lys Phe Asn Lys Trp Gly Arg 
            660                 665                 670         


Leu Ser Lys Arg Leu Leu Thr Glu Ile Cys Phe Glu Asn Val Ile Glu 
        675                 680                 685             


Ser Glu Ser Phe Glu Asn Ile Ile Val Ala Leu Arg Ser Thr Asn Tyr 
    690                 695                 700                 


Asn Phe Met Glu Phe Thr Ser Lys Lys Phe Asn Leu Ser Glu Lys Val 
705                 710                 715                 720 


Ala Glu Glu Asn Gln Glu Asn Thr Leu Glu Lys Phe Asn Ile Asn Glu 
                725                 730                 735     


Tyr Val Asp Glu Leu Tyr Val Ser Pro Ser Val Lys Arg Ser Ile Ile 
            740                 745                 750         


Gln Thr Tyr Lys Ile Ala Met Glu Ile Lys Lys Ile Thr Gly Lys Asn 
        755                 760                 765             


Pro Lys Lys Ile Phe Ile Glu Met Ala Arg Gly Gly Gly Glu Lys Gly 
    770                 775                 780                 


Lys Lys Thr Glu Lys Arg Lys Glu Arg Ile Lys Thr Leu Tyr Ala Asn 
785                 790                 795                 800 


Met Lys Asp Ser Asn Glu Glu Phe Leu Asn Asn Leu Asp Asn Leu Lys 
                805                 810                 815     


Leu Ser Val Asp Asn Tyr Asp Asp Asn Arg Leu Arg Glu Lys Lys Leu 
            820                 825                 830         


Phe Leu Tyr Phe Met Gln Leu Gly Lys Ser Met Tyr Thr Gly Lys Asn 
        835                 840                 845             


Ile Asp Leu Asn Glu Leu Leu Phe Ala Asn Thr Tyr Asp Ile Asp His 
    850                 855                 860                 


Ile Ile Pro Gln Ser Lys Leu Lys Asp Asp Ser Phe Asp Asn Ile Val 
865                 870                 875                 880 


Leu Val Glu Lys Thr Ile Asn Lys Asp Lys Asp Asn Arg Asp Tyr Leu 
                885                 890                 895     


Asp Glu Ser Ile Arg Leu Arg Met Asn Lys Phe Trp Asn Phe Leu Arg 
            900                 905                 910         


Glu Lys Gly Phe Ile Thr Ile Glu Lys Tyr Asn Arg Leu Arg Arg Asn 
        915                 920                 925             


Thr Tyr Phe Ser Asp Asp Glu Leu Ser Gly Phe Ile Ser Arg Gln Leu 
    930                 935                 940                 


Val Thr Thr Arg Gln Ser Thr Leu Glu Val Lys Lys Leu Phe Glu Phe 
945                 950                 955                 960 


Ile Phe Gln Asn Thr Asp Val Val Cys Ser Lys Ala Asn Leu Val Ser 
                965                 970                 975     


Asp Phe Arg Glu Lys Tyr Gly Ile Ile Lys Val Arg Asp Leu Asn Asp 
            980                 985                 990         


Ala His His Ala Gln Asp Ala Tyr  Leu Asn Ile Val Val  Gly Asn Val 
        995                 1000                 1005             


Phe Asp  Thr Lys Phe Thr Lys  Asn Tyr Trp Lys Phe  Ile Glu Asp 
    1010                 1015                 1020             


Arg Arg  Lys Val Asp Asn Ile  Arg Glu Thr Tyr Asn  Phe Lys Lys 
    1025                 1030                 1035             


Ile Phe  Asp Tyr Asp Val Ser  Arg Gly Glu Asn Val  Ala Trp Leu 
    1040                 1045                 1050             


Lys Asp  Tyr Ser Ile Asn Thr  Ile Ser Glu Met Ile  Asn Lys Lys 
    1055                 1060                 1065             


Thr Ala  Asn Ile Thr Gln Met  Ile Thr Glu Gly Lys  Gly Gln Leu 
    1070                 1075                 1080             


Phe Asp  Leu Thr Ile Leu Lys  Lys Ser Lys Ile Asp  Lys Lys Leu 
    1085                 1090                 1095             


Thr Val  Ile Pro Asn Thr Pro  Lys Ile Leu Lys Ser  Ser Leu Lys 
    1100                 1105                 1110             


Asp Ser  Cys Lys Ile Ala Glu  Lys Tyr Gly Tyr Phe  Ser Thr Leu 
    1115                 1120                 1125             


Met Pro  Ala Tyr Phe Val Val  Phe Lys Tyr Ile Asp  Lys Asn Lys 
    1130                 1135                 1140             


Glu Val  Phe Gly Phe Asp Arg  Ile Phe Ile Phe Asp  Arg Glu Lys 
    1145                 1150                 1155             


Ile Asn  Ser Asn Glu Asp Ile  Glu Lys Tyr Leu Glu  Asp Lys Gly 
    1160                 1165                 1170             


Tyr Asn  Ser Pro Lys Phe Ile  Arg Lys Val Asn Leu  Lys Gln Lys 
    1175                 1180                 1185             


Ile Leu  Leu Asn Gly Tyr Pro  Tyr Arg Ile Val Gly  Phe Thr Asp 
    1190                 1195                 1200             


Asn Lys  Leu Glu Ile Lys Asn  Ser Lys Gln Phe Tyr  Ser Asp Gly 
    1205                 1210                 1215             


Glu His  Leu Lys Tyr Ile Lys  His Leu Ser Ala Phe  Tyr Asn Asn 
    1220                 1225                 1230             


Asn Asn  Asn Val Lys Asn Gly  Glu Tyr Arg Ile Asn  Phe Leu Lys 
    1235                 1240                 1245             


Lys Thr  Ser Glu Asn Asp Arg  Asn Glu Thr Thr Asp  Glu Ala Lys 
    1250                 1255                 1260             


Glu Arg  Leu Asn Ile Glu Phe  Ile Lys Leu Tyr Glu  Tyr Phe Cys 
    1265                 1270                 1275             


Glu Arg  Leu Asn Ser Lys Ile  Tyr Ala Asn Tyr Tyr  Asn Ser Lys 
    1280                 1285                 1290             


Lys Tyr  Asp Glu Leu Ile Glu  Val Phe Asp Cys Phe  Lys Asn Leu 
    1295                 1300                 1305             


Asn Leu  Leu Asp Lys Ala Lys  Leu Leu Leu Glu Phe  Leu Lys Ile 
    1310                 1315                 1320             


Phe Lys  Lys Asp Thr Tyr Gly  Asn Phe Lys Leu Leu  Glu Lys Pro 
    1325                 1330                 1335             


Glu Leu  Asp Lys Asn Gly Lys  Gln Lys Leu Asp Lys  Asn Gly Asn 
    1340                 1345                 1350             


Leu Ser  Phe Glu Gly Leu Ser  Met Ala Phe Gly Lys  Leu Asn Gly 
    1355                 1360                 1365             


Lys Lys  Phe Lys Gln Leu Val  Glu Lys Asp Ile Gln  Phe Val Asp 
    1370                 1375                 1380             


Glu Ser  Val Thr Gly Leu Phe  Glu Lys Lys Glu Gly  Leu Glu Lys 
    1385                 1390                 1395             


Trp Gln  Asp Gly Glu Gln 
    1400                 


<210>  1189
<211>  1404
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1189

Met Ala Lys Lys Ile Leu Gly Leu Asp Leu Gly Val Ser Ser Ile Gly 
1               5                   10                  15      


Trp Ala Ile Val Glu Glu Asp Ala Glu Asn Val Met Asn Asn Lys Ile 
            20                  25                  30          


Val Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Gln Ile 
        35                  40                  45              


Asn Phe Glu Lys Gly Lys Pro Ile Thr Thr Asn Ala Gly Arg Thr Leu 
    50                  55                  60                  


Val Arg Gly Ala Arg Arg Asn Leu Gln Arg Phe Lys Leu Arg Arg Leu 
65                  70                  75                  80  


His Leu Ile Asp Ile Leu Gln Lys Arg Asn Ile Leu Lys Gln Ala Asp 
                85                  90                  95      


Leu Leu Ala Glu Ser Gly Arg Asn Ser Thr Phe Gln Thr Gln Glu Ile 
            100                 105                 110         


Arg Ala Lys Ser Ala Lys Gln Lys Val Glu Leu Ser Glu Leu Ala Arg 
        115                 120                 125             


Val Leu Leu Leu Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys 
    130                 135                 140                 


Ala Lys Asn Glu Asp Glu Gly Gln Ile Val Asp Gly Met Ser Val Ala 
145                 150                 155                 160 


Lys Lys Leu Tyr Glu Glu Asn Thr Thr Pro Gly Glu Tyr Ser Tyr Glu 
                165                 170                 175     


Leu Leu Lys Ser Gly Lys Lys Gln Leu Ser Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         


Leu Gln Ala Glu Phe Asp Arg Val Trp Asn Phe Gln Lys Gln Phe Tyr 
        195                 200                 205             


Pro Glu Ile Phe Ser Asp Asn Leu Tyr Lys Glu Leu Gln Gly Lys Asn 
    210                 215                 220                 


Lys Asn Ala Thr Trp Lys Ile Leu Glu Ile Pro Phe Ser Leu Val Gly 
225                 230                 235                 240 


Ile Lys Gln Val Gly Thr Thr Gln Glu Lys Lys Ile Glu Lys Tyr Phe 
                245                 250                 255     


Trp Arg Ser Glu Ala Val Lys Lys Gln Leu Asp Phe Glu Ser Leu Ala 
            260                 265                 270         


Val Val Phe Gln Glu Ile Asn Ser Asn Leu Asn Asn Ser Ser Gly Tyr 
        275                 280                 285             


Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Gln 
    290                 295                 300                 


Thr Val Gly Glu Tyr Leu His Cys Gln Leu Lys Glu Asn Pro His Thr 
305                 310                 315                 320 


Lys Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe 
                325                 330                 335     


Glu Lys Ile Trp Glu Ile Gln Ser Lys Tyr His Thr Val Leu Thr Lys 
            340                 345                 350         


Glu Leu Lys Glu Glu Ile Arg Asp Val Ile Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Gly Leu Ile Ser Ile Cys Glu Phe Glu Asn Arg 
    370                 375                 380                 


Glu Ile Glu Ile Thr Glu Asn Gly Lys Ile Lys Lys Lys Glu Val Gly 
385                 390                 395                 400 


Leu Lys Val Ala Pro Lys Ser Ser Leu Leu Phe Gln Glu Phe Lys Ile 
                405                 410                 415     


Trp Gln Val Leu Asn Asn Leu Gln Phe Gln Asn Ile Glu Ser Lys Glu 
            420                 425                 430         


Ile Phe Pro Leu Asp Leu Asp Cys Lys Glu Ser Ile Phe Asn Glu Val 
        435                 440                 445             


Asn Ile Lys Gly Arg Ile Ser Ala Lys Ala Val Leu Asp Val Leu Gly 
    450                 455                 460                 


Tyr Ser Gly Lys Glu Trp Lys Thr Asn Phe Lys Asp Ile Glu Gly Asn 
465                 470                 475                 480 


Asn Ser Asn Glu Asn Leu Tyr Asn Ala Tyr Leu Lys Ile Ile Ala Asn 
                485                 490                 495     


Glu Gly Ile Glu Phe Pro Lys Glu Phe Lys Cys Asn Leu Asp Asp Asp 
            500                 505                 510         


Ile Asn Val Ser Lys Ile Ile Cys Ser Ala Gly Lys Ile Ile Glu Phe 
        515                 520                 525             


Val Arg Glu Lys Phe Ser Glu Leu Gly Ile Asn Thr Ser Ile Leu Asp 
    530                 535                 540                 


Phe Asn Ser Glu Leu Glu Gly Thr Asn Phe Glu Lys Gln Lys Ser Tyr 
545                 550                 555                 560 


Gln Leu Trp His Leu Leu Tyr Ser Tyr Asp Gly Asp Asp Ser Pro Ser 
                565                 570                 575     


Gly Asn Gln Lys Leu Tyr Glu Leu Leu Glu Lys Lys Phe Gly Phe Lys 
            580                 585                 590         


Lys Glu His Ser Ile Ile Leu Ala Asp Ile Gly Phe Pro Gln Glu Tyr 
        595                 600                 605             


Gly Ser Leu Ser Thr Lys Ala Met Arg Lys Ile Ile Pro His Leu Lys 
    610                 615                 620                 


Thr Gly Leu Pro Phe Ala Lys Lys Ser Asp Asn Asp Thr Ile Leu Ser 
625                 630                 635                 640 


Ala Thr Ala Met Ala Gly Tyr Asn His Ser Lys Ser Leu Thr Thr Glu 
                645                 650                 655     


Glu Leu Thr Lys Arg Val Leu Lys Glu His Leu Glu Ile Leu Pro Lys 
            660                 665                 670         


Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Met Ile 
        675                 680                 685             


Asn Val Val Asn Glu Val Ile Lys Glu Tyr Gly Lys Pro Asp Glu Ile 
    690                 695                 700                 


Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Glu Glu Arg Ala 
705                 710                 715                 720 


Asn Met Thr Ser Glu Ile Gly Lys Ala Thr Ile Gln His Gln Lys Tyr 
                725                 730                 735     


Ser Glu Ile Leu Gln Lys Glu Phe Gly Ile Lys Ser Pro Ser Arg Asn 
            740                 745                 750         


Asp Ile Ile Arg Tyr Lys Leu Tyr Leu Glu Leu Ser Asn Asn Gly Phe 
        755                 760                 765             


Lys Asp Leu Tyr Thr Asp Ile Gln Ile Lys Arg Glu Glu Leu Phe Thr 
    770                 775                 780                 


Asp Lys Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Arg Phe Phe Asp 
785                 790                 795                 800 


Asp Ser Phe Ser Asn Lys Val Leu Val Pro Arg Gly Ala Asn Leu Lys 
                805                 810                 815     


Lys Gly Asn Leu Thr Ala Phe Asp Tyr Leu Glu Ser Glu Gly Lys Asp 
            820                 825                 830         


Thr Leu Glu Lys Phe Ile Asn Thr Ile Thr Glu Phe Tyr Asp Lys Gly 
        835                 840                 845             


Ile Ile Thr Lys Ala Lys Tyr Glu Lys Leu Gln Lys Lys Gly Thr Glu 
    850                 855                 860                 


Ile Gly Asp Gly Phe Ile Glu Arg Asp Leu Arg Asp Thr Gln Tyr Ile 
865                 870                 875                 880 


Ala Lys Lys Ala Lys Glu Ile Leu Phe Gly Ile Thr Asn Ser Val Val 
                885                 890                 895     


Ser Thr Ser Gly Arg Ile Thr Asp Lys Leu Arg Glu Asp Trp Asn Leu 
            900                 905                 910         


Val Asn Thr Met Lys Glu Met Asn Leu Glu Lys Tyr Arg Lys Arg Gly 
        915                 920                 925             


Leu Thr Glu Thr Val Ile Asn Ser Lys Gly Glu Glu Lys Glu Lys Ile 
    930                 935                 940                 


Ile Asp Trp Ser Lys Arg Asn Asp His Arg His His Ala Met Asp Ala 
945                 950                 955                 960 


Leu Thr Val Ala Phe Thr Thr His Asn His Ile Gln Tyr Leu Asn Tyr 
                965                 970                 975     


Leu Asn Ala Arg Lys Asn Glu Asp His Lys Gln His Lys Val Ile Ser 
            980                 985                 990         


Asn Ile Glu Asn Leu Ile Thr Lys  Val Phe Glu Lys Lys  Asn Gly Ser 
        995                 1000                 1005             


Lys Gln  Arg Arg Phe Leu Glu  Pro Ile His Asn Phe  Arg Ile Asp 
    1010                 1015                 1020             


Ala Lys  Lys His Leu Glu Glu  Ile Leu Ile Ser His  Lys Ala Lys 
    1025                 1030                 1035             


Asn Lys  Val Val Thr Lys Asn  Ile Asn Lys Ile Lys  Lys Lys Gly 
    1040                 1045                 1050             


Ser Ile  Ile Ala Lys Ile Glu  Leu Thr Pro Arg Gly  Gln Leu His 
    1055                 1060                 1065             


Lys Glu  Thr Ile Tyr Gly Ser  Ser Lys Tyr Leu Lys  Thr Lys Asp 
    1070                 1075                 1080             


Glu Lys  Leu Ser Gly Lys Phe  Asp Leu Glu Thr Ile  Asn Lys Val 
    1085                 1090                 1095             


Gln Asn  Glu Arg Tyr Lys Asn  Ala Leu Leu Glu Arg  Leu Lys Val 
    1100                 1105                 1110             


Phe Gly  Tyr Asp Ser Lys Lys  Ala Phe Thr Gly Lys  Asn Ile Leu 
    1115                 1120                 1125             


Ala Lys  Asn Pro Ile Phe Leu  Asn Ile Glu Lys Thr  Glu Gln Leu 
    1130                 1135                 1140             


Pro Glu  Val Leu Thr Leu Ala  Trp Tyr Glu Thr Ile  Tyr Thr Ile 
    1145                 1150                 1155             


Arg Lys  Ser Ile Asn Ser Asp  Asn Phe Lys Asp Tyr  Lys Asn Ile 
    1160                 1165                 1170             


Asp Lys  Val Thr Asp Lys Lys  Val His Asn Ile Leu  Thr Asp Arg 
    1175                 1180                 1185             


Leu Arg  Glu Phe Asn Gly Asn  Ser Lys Glu Ala Phe  Ser Asp Leu 
    1190                 1195                 1200             


Glu Lys  Asn Pro Ile Trp Leu  Asn Lys Pro Lys Gly  Ile Ala Ile 
    1205                 1210                 1215             


Lys Thr  Val Thr Ile Thr Gly  Val Asn Asn Val Glu  Ala Leu His 
    1220                 1225                 1230             


Tyr Lys  Lys Asp His Phe Gly  Lys Glu Phe Leu Asp  Glu Lys Gly 
    1235                 1240                 1245             


His Lys  Ile Pro Val Asp Phe  Val Ser Thr Gly Asn  Asn His His 
    1250                 1255                 1260             


Val Ala  Ile Tyr Glu Asp Thr  Ala Gly Asn Leu Gln  Glu Arg Val 
    1265                 1270                 1275             


Leu Ser  Phe Tyr Glu Ala Val  Glu Arg Val Asn Gln  Gly Leu Ser 
    1280                 1285                 1290             


Val Ile  Asp Lys Glu Tyr Asn  Ser Ser Leu Gly Trp  Lys Phe Leu 
    1295                 1300                 1305             


Phe Thr  Met Lys Gln Asn Glu  Met Phe Leu Phe His  Ser Glu Asp 
    1310                 1315                 1320             


Phe Asn  Pro Gln Glu Ile Asp  Leu Tyr Asp Glu Lys  Asn Leu Arg 
    1325                 1330                 1335             


Leu Ile  Ser Lys Asn Leu Phe  Arg Val Gln Lys Phe  Thr Val Lys 
    1340                 1345                 1350             


Asp Tyr  Phe Phe Arg His His  Leu Glu Thr Asn Val  Glu Asp Asn 
    1355                 1360                 1365             


Ser Lys  Leu Lys Gly Asp Thr  Trp Lys Arg Glu Gly  Leu Asn Gly 
    1370                 1375                 1380             


Ile Arg  Gly Ile Leu Lys Val  Arg Leu Asn His Leu  Gly Lys Ile 
    1385                 1390                 1395             


Val Gln  Val Gly Glu Tyr 
    1400                 


<210>  1190
<211>  1404
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1190

Met Ser Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly 
1               5                   10                  15      


Trp Ala Phe Val Arg Glu Ala Glu Asp Phe Gln Glu Glu Ser Ser Ile 
            20                  25                  30          


Glu Arg Ile Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Gln Ile 
        35                  40                  45              


Asn Phe Glu Lys Gly Lys Pro Ile Ser Val Asn Ala Asp Arg Thr Leu 
    50                  55                  60                  


Lys Arg Gly Ala Arg Arg Asn Leu Gln Arg Tyr Lys Gln Arg Arg Thr 
65                  70                  75                  80  


Asn Leu Ile Gln Leu Leu Lys Gln Tyr Lys Ile Ile Asn Glu Asn Thr 
                85                  90                  95      


Ile Leu Ala Glu Asp Asp Lys Asn Thr Thr His Ser Thr Tyr Ala Leu 
            100                 105                 110         


Arg Ala Lys Ala Val Ser Glu Lys Ile Ser Lys Glu Ala Phe Ala Arg 
        115                 120                 125             


Val Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys 
    130                 135                 140                 


Ala Lys Asn Glu Asp Glu Gly Gln Ala Ile Asp Gly Met Ser Val Ala 
145                 150                 155                 160 


Lys Arg Leu Tyr Glu Glu Asp Ile Thr Pro Gly Gln Leu Cys Leu Gln 
                165                 170                 175     


Leu Leu Lys Glu Gly Lys Lys Lys Leu Pro Asp Phe Tyr Leu Ser Asp 
            180                 185                 190         


Leu Gln Ala Glu Leu Glu Lys Ile Trp Asn Phe Gln Lys Lys Phe Tyr 
        195                 200                 205             


Pro Glu Ile Leu Thr Asp Glu Phe Lys Lys Glu Ile Glu Gly Lys Gly 
    210                 215                 220                 


Gln Arg Ala Thr Ser Ala Ser Phe Trp Ala Lys Tyr Lys Phe Asn Thr 
225                 230                 235                 240 


Ala Glu Asn Lys Gly Ser Arg Asp Glu Lys Lys Met Gln Ala Trp Glu 
                245                 250                 255     


Trp Arg Ser Lys Ala Ile Asn Thr Gln Leu Thr Arg Glu Glu Val Ala 
            260                 265                 270         


Tyr Val Ile Thr Glu Ile Asn Asn Asn Ile Asn Asn Ser Ser Gly Tyr 
        275                 280                 285             


Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Glu 
    290                 295                 300                 


Thr Val Gly Glu Tyr Leu Phe Arg Gln Leu Arg Tyr Ser Pro His Thr 
305                 310                 315                 320 


Arg Val Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe 
                325                 330                 335     


Glu Thr Ile Trp Glu Asn Gln Ser Arg Tyr His Asn Glu Leu Thr Asn 
            340                 345                 350         


Glu Leu Lys Ser Glu Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Lys 
    370                 375                 380                 


Gln Ile Glu Ile Gln Lys Asp Gly Lys Ile Val Lys Lys Ile Ile Gly 
385                 390                 395                 400 


Leu Lys Val Ser Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile 
                405                 410                 415     


Trp Gln Ile Leu Gln Asn Leu Glu Phe Thr Asn Lys Glu Thr Lys Glu 
            420                 425                 430         


Lys Lys Val Pro Asp Phe Glu Ala Lys Asn Leu Leu Phe Glu Glu Leu 
        435                 440                 445             


Asn Ile Lys Gly Asn Leu Lys Gly Glu Lys Ala Ile Glu Ile Leu Gly 
    450                 455                 460                 


Tyr Lys Pro Lys Glu Trp Leu Val Asn Tyr Lys Glu Leu Asp Gly Asn 
465                 470                 475                 480 


Arg Thr Asn Gln Val Leu Tyr Glu Gly Tyr Leu Lys Ile Leu Glu Val 
                485                 490                 495     


Glu Gly Tyr Asp Ile Lys Asp Leu Leu Lys Ile Lys Ser Asn Lys Asp 
            500                 505                 510         


Glu Val Asn Leu Ser Glu Leu Ser Lys Pro Val Ser Glu Ile Lys Asp 
        515                 520                 525             


Leu Ile Lys Ser Ile Phe Glu Ser Leu Asn Ile Asn Thr Ala Ile Leu 
    530                 535                 540                 


Asp Phe Asn Pro Glu Leu Glu Gly Lys Asp Phe Glu Arg Gln Ala Ser 
545                 550                 555                 560 


Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asn Ser Asn 
                565                 570                 575     


Thr Gly Asn Asp Glu Leu Tyr Arg Leu Leu Tyr Gln Lys Phe Gly Phe 
            580                 585                 590         


Gly Lys Glu His Ala Gln Ile Leu Ala Asn Leu Ser Phe Ser Gln Asp 
        595                 600                 605             


Tyr Gly Ser Leu Ser Ala Lys Ala Met Arg Lys Ile Tyr Pro Phe Ile 
    610                 615                 620                 


Lys Glu Asn Ser Tyr Ser Glu Ala Cys Ser Leu Ala Gly Tyr Asn His 
625                 630                 635                 640 


Ser Ser Ser Ile Thr Lys Glu Glu Asn Asp Lys Arg Glu Leu Lys Glu 
                645                 650                 655     


Lys Leu Glu Leu Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
            660                 665                 670         


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Ala Ile Ile Ala Asp 
        675                 680                 685             


Pro Ser Met Gly Arg Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu 
    690                 695                 700                 


Leu Lys Lys Asn Ala Ala Glu Arg Ala Glu Met Thr Ile His Ile Asn 
705                 710                 715                 720 


Lys Ala Lys Ser Glu His Asp Asn Tyr Arg Lys Ile Leu Thr Thr Glu 
                725                 730                 735     


Phe Gly Ile Pro Asn Pro Thr Arg Asn Asp Ile Ile Arg Tyr Lys Leu 
            740                 745                 750         


Tyr Glu Glu Leu Lys Val Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Thr 
        755                 760                 765             


Tyr Ile Pro Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Val Glu His 
    770                 775                 780                 


Ile Ile Pro Lys Ala Leu Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr 
785                 790                 795                 800 


Ile Cys Ala Arg Gln Glu Asn Leu Asp Lys Gly Asn Lys Thr Ala Phe 
                805                 810                 815     


Asp Phe Ile Gln Ser Phe Tyr Gly Lys Glu Lys Phe Glu Glu Tyr Leu 
            820                 825                 830         


Gln Arg Ile Glu Ser Leu Ala Lys Ile Lys Glu Gly Asp Thr Phe Val 
        835                 840                 845             


Ile Ser Lys Ala Lys Tyr Lys Lys Leu Leu Leu Pro Glu Ile Glu Ile 
    850                 855                 860                 


Gly Glu Gly Phe Ile Glu Arg Asp Leu Arg Asn Ser Gln Tyr Ile Ala 
865                 870                 875                 880 


Lys Lys Ala Lys Ser Met Leu Leu Asp Ile Cys Lys Thr Val Val Pro 
                885                 890                 895     


Thr Thr Gly Ser Ile Thr Asp Arg Leu Arg Glu Asp Trp Gly Leu Ile 
            900                 905                 910         


Asn Ile Met Gln Glu Leu Asn Phe Glu Lys Tyr Lys Lys Leu Gly Leu 
        915                 920                 925             


Ile Glu Met Val Glu Lys Lys Asp Gly Ser Phe Lys Glu Arg Ile Thr 
    930                 935                 940                 


Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu 
945                 950                 955                 960 


Thr Val Ala Phe Thr Lys His Asn His Ile Gln Tyr Leu Asn Tyr Leu 
                965                 970                 975     


Asn Ala Arg Lys Asn Glu Asn His Lys Met His Gly Asn Val Ile Ala 
            980                 985                 990         


Ile Glu Gln Lys Glu Thr Glu Leu  Leu Val Asp Ser Val  Gly Lys Arg 
        995                 1000                 1005             


Lys Arg  Lys Phe Lys Leu Pro  Ile Ser Asn Phe Arg  Glu Val Ala 
    1010                 1015                 1020             


Lys Met  His Leu Glu Asn Ile  Leu Val Ser Tyr Lys  Ala Lys Asn 
    1025                 1030                 1035             


Lys Val  Ala Thr Lys Asn Lys  Asn Arg Phe Lys Thr  Lys Asn Gly 
    1040                 1045                 1050             


Glu Lys  Val Lys Ile Glu Leu  Thr Pro Arg Gly Gln  Leu His Lys 
    1055                 1060                 1065             


Glu Thr  Val Tyr Gly Lys Met  Lys Lys Tyr Glu Thr  Lys Glu Glu 
    1070                 1075                 1080             


Ile Ile  Gly Ser Lys Phe Asp  Glu Ala Thr Ile Leu  Lys Val Ala 
    1085                 1090                 1095             


Asn Leu  Glu Gln Arg Asn Ala  Leu Leu Gln Arg Leu  Lys Glu Asn 
    1100                 1105                 1110             


Asn Asn  Asp Pro Lys Lys Ala  Phe Ser Gly Lys Asn  Ser Leu Lys 
    1115                 1120                 1125             


Ser Asn  Pro Leu Tyr Tyr Gly  Val Asp Ser Lys Pro  Ile Pro Glu 
    1130                 1135                 1140             


Arg Ile  Lys Ile Val Trp Leu  Glu Asp Asp Tyr Thr  Ile Arg Lys 
    1145                 1150                 1155             


Glu Ile  Thr Pro Asp Leu Lys  Ile Asp Lys Val Ile  Asp Ile Gly 
    1160                 1165                 1170             


Ile Arg  Arg Ile Leu Glu Asn  Arg Leu Ala Glu Phe  Asn Gly Asn 
    1175                 1180                 1185             


Ala Lys  Glu Ala Phe Ser Asn  Leu Glu Lys Asn Pro  Ile Trp Leu 
    1190                 1195                 1200             


Asn Glu  Gly Lys Arg Ile Ala  Ile Lys Arg Val Thr  Ile Ser Gly 
    1205                 1210                 1215             


Val Lys  Asn Ala Glu Thr Leu  His Tyr Lys Lys Asp  His Leu Gly 
    1220                 1225                 1230             


His Glu  Met Leu Asp Lys Gln  Gly Lys Arg Ile Pro  Val Asp Phe 
    1235                 1240                 1245             


Val Ser  Thr Gly Asn Asn His  His Val Ala Ile Tyr  Lys Asp Asp 
    1250                 1255                 1260             


Lys Ser  Asp Leu Gln Glu Lys  Val Val Ser Phe Tyr  Glu Ala Val 
    1265                 1270                 1275             


Glu Arg  Ile Asn Gln Glu Leu  Pro Ile Ile Asp Lys  Ser Phe Asn 
    1280                 1285                 1290             


His Asp  Leu Gly Trp Gln Phe  Leu Phe Thr Met Lys  Gln Asn Glu 
    1295                 1300                 1305             


Tyr Phe  Val Phe Pro Arg Asp  Gly Phe Asp Pro Tyr  Glu Ile Asp 
    1310                 1315                 1320             


Leu Leu  Asn Pro Asp Asn Tyr  Lys Ile Ile Ser Pro  Asn Leu Phe 
    1325                 1330                 1335             


Arg Val  Gln Lys Ile Ala Thr  Lys Asn Tyr Val Phe  Asn His His 
    1340                 1345                 1350             


Leu Glu  Thr Lys Ala Val Asp  Gly Glu Leu Leu Lys  Thr Lys Lys 
    1355                 1360                 1365             


Asp Leu  Ser Asp Ile Thr Tyr  Arg Phe Ile Gln Thr  Pro Val Asn 
    1370                 1375                 1380             


Leu Lys  Asn Ile Ile Lys Val  Arg Ile Asn His Ile  Gly Gln Ile 
    1385                 1390                 1395             


Val Lys  Ile Gly Glu Tyr 
    1400                 


<210>  1191
<211>  1405
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1191

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Glu Ala Glu Asn Ser Asn Glu Lys Ser Glu Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Ala Gly Arg Pro Leu Ser Thr Asn Ala Asp Arg Thr Ala Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Lys Asn 
65                  70                  75                  80  


Leu Ile Asp Leu Leu Ile Lys Asn Gln Leu Ile Asp Lys Asp Thr Pro 
                85                  90                  95      


Leu Thr Glu Ile Gly Lys Asn Thr Thr His Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Arg Glu Arg Ile Glu Leu Glu Asp Leu Ala Arg Val 
        115                 120                 125             


Phe Leu Ala Ile Asn Lys Lys Arg Gly Tyr Arg Ser Ser Arg Lys Val 
    130                 135                 140                 


Asn Asn Glu Glu Glu Gly Gln Ala Val Asp Gly Met Ala Val Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Asp Glu Asn Leu Thr Pro Gly Gln Tyr Ala Tyr Glu Leu 
                165                 170                 175     


Leu Ser Lys Gly Lys Lys Tyr Val Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asn Ala Ile Trp Glu Tyr Gln Lys Gln Phe Tyr Ala 
        195                 200                 205             


Asp Ile Leu Asp Asp Glu Leu Tyr Asn Ala Leu Lys Gly Gln Gly Gln 
    210                 215                 220                 


Gln Asn Ser Arg Lys Arg Phe Leu Ala Ile Lys Gly Val Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Lys Arg Asp Asp Val Lys Leu Arg His Tyr Lys Trp 
                245                 250                 255     


Arg Ser Glu Ala Ile Ser Lys Lys Ile Asn Ile Glu Glu Val Ala Tyr 
            260                 265                 270         


Val Leu Val Glu Ile Asn Asn Asp Leu Asn Lys Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Glu Thr 
    290                 295                 300                 


Val Gly Glu Asn Leu Trp Lys Gln Ile Gln Lys Asn Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Glu Thr Gln Ala Gln Phe His Pro Gln Leu Thr Leu Ala 
            340                 345                 350         


Leu Lys Glu Gln Ile Arg Asp Val Ile Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys Gly Leu Leu Ser Phe Cys Gln Phe Glu Ser Trp Glu 
    370                 375                 380                 


Ile Glu Arg Lys Asp Glu Asn Gly Asn Val Ile Leu Asn Lys Thr Thr 
385                 390                 395                 400 


Gln Leu Pro Lys Arg Gln Thr Val Gly Arg Arg Val Ala Pro Lys Ser 
                405                 410                 415     


Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Asn Ile Asn Asn Leu 
            420                 425                 430         


Glu Val Thr Lys Ile Ser Glu Gly Ala Ser Lys Asn Lys Lys Glu Thr 
        435                 440                 445             


Glu Thr Leu Ser Asp Asp Glu Arg Lys Leu Leu Phe Glu Glu Leu Asn 
    450                 455                 460                 


Leu Arg Gly Asn Leu Ser Gln Lys Glu Val Leu Gln Ile Leu Gly Leu 
465                 470                 475                 480 


Lys Asp Lys Glu Tyr Lys Thr Asn Phe Ser Glu Gly Leu Glu Gly Asn 
                485                 490                 495     


Arg Thr Asn Ala Ala Leu Phe Asn Ile Tyr Gln Gln Ile Ala Glu Asn 
            500                 505                 510         


Glu Gly Tyr Gly Phe Asp Trp Ser Lys Lys Ser Ala Gln Glu Ile Lys 
        515                 520                 525             


Glu Glu Leu Lys Ala Val Phe Pro Gln Ile Gly Ile Gln Ala Asp Ile 
    530                 535                 540                 


Leu Asp Phe Asn Ala Asn Leu Asp Gly Lys Glu Phe Glu Asn Gln Ala 
545                 550                 555                 560 


Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Ala Glu Glu Asp Asp Lys 
                565                 570                 575     


Ile Asn Glu Glu Asp Gln Ile Ile Tyr Gly Asn Ser Ala Val Ser Leu 
            580                 585                 590         


Lys Lys Lys Leu Cys Glu Lys Phe Gly Phe Thr Pro Glu Tyr Ala Lys 
        595                 600                 605             


Trp Ile Ala Asn Val Ser Leu Gln Asp Asp Tyr Gly Asn Leu Ser Thr 
    610                 615                 620                 


Lys Ala Met Arg Lys Ile Ile Pro Tyr Leu Ile Asp Gly Asn Asp Tyr 
625                 630                 635                 640 


Ser Glu Ala Cys Ala Leu Ala Gly Tyr Asn His Ser Asn Ser Leu Thr 
                645                 650                 655     


Lys Glu Glu Asn Asp Asn Arg Glu Leu Leu Asp Lys Leu Glu Leu Leu 
            660                 665                 670         


Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln 
        675                 680                 685             


Met Val Asn Val Val Asn Gln Val Ile Glu Thr Tyr Gly Lys Pro Asp 
    690                 695                 700                 


Glu Val Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Ser Ala Glu Lys 
705                 710                 715                 720 


Arg Ala Glu Met Thr Lys Ser Ile Asn Glu Ala Thr Arg Arg Asn Glu 
                725                 730                 735     


Asp Ile Lys Lys Leu Ile Thr Lys Asp Phe Gly Ile Pro Asn Pro Thr 
            740                 745                 750         


Lys Ser Asp Val Val Arg Tyr Arg Leu Trp Glu Glu Leu Ala Pro Leu 
        755                 760                 765             


Ala Tyr Lys Asp Val Phe Thr Gly Lys Gln Ile Lys Lys Glu Asp Leu 
    770                 775                 780                 


Phe Ser Ser Lys Ile Asp Ile Glu His Ile Ile Pro Lys Ala Leu Leu 
785                 790                 795                 800 


Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Val Phe Arg Glu Thr Asn 
                805                 810                 815     


Leu Lys Lys Ala Asp Arg Thr Ala Tyr Asp Phe Ile Glu Ser Asp Tyr 
            820                 825                 830         


Asn Ala Thr Leu Asp Asp Tyr Ile Gln Arg Val Glu Thr Leu Tyr Asn 
        835                 840                 845             


Asn Thr Lys Gly Thr Ile Ser Lys Gly Lys Arg Asn Lys Leu Leu Met 
    850                 855                 860                 


Ala Gln Lys Asn Leu Pro Asp Gly Phe Ile Glu Arg Asp Leu Arg Asn 
865                 870                 875                 880 


Ser Gln Tyr Ile Ala Lys Lys Ala Lys Ala Met Leu Tyr Gln Val Phe 
                885                 890                 895     


Lys Asp Val Asn Val Thr Ser Gly Ser Ile Thr Asp Arg Leu Arg Glu 
            900                 905                 910         


Asp Trp Asp Leu Ile Asn Val Met Lys Glu Leu Asn Phe Pro Lys Tyr 
        915                 920                 925             


Lys Ala Leu Gly Leu Thr Glu Ile Glu Glu Arg Tyr Asp Ile Gly Gln 
    930                 935                 940                 


Glu Lys Thr Lys Arg Val Glu Val Ile Thr Asp Trp Thr Lys Arg Asn 
945                 950                 955                 960 


Asp His Arg His His Ala Met Asn Ala Leu Thr Val Ala Phe Thr Thr 
                965                 970                 975     


Ser Ser His Ile Gln Tyr Leu Asn Tyr Leu Asn Ala Arg Lys Asn Glu 
            980                 985                 990         


Ser His Lys Glu His Lys Asn Ile  Lys Gly Ile Glu Asn  Lys Ile Thr 
        995                 1000                 1005             


His Lys  Gln Lys Asp Lys Arg  Lys Phe Ile Pro Pro  Met Pro Asn 
    1010                 1015                 1020             


Phe Arg  Glu Glu Ala Lys Lys  His Ile Glu Thr Ile  Leu Val Ser 
    1025                 1030                 1035             


Ile Lys  Asn Lys Asn Lys Val  Tyr Thr Lys Asn Ile  Asn Lys Thr 
    1040                 1045                 1050             


Lys Lys  Lys Ser Gly His Asn  Thr Lys Glu Gln Leu  Thr Pro Arg 
    1055                 1060                 1065             


Gly Gln  Leu His Lys Glu Thr  Val Tyr Gly Lys Ser  Lys Arg Pro 
    1070                 1075                 1080             


Met Ser  Lys Pro Thr Lys Ile  Asn Lys Asn Phe Ser  Leu Glu Gln 
    1085                 1090                 1095             


Ala Gln  Leu Ile Ile Asn Val  Gln Glu Lys Glu Leu  Val Leu Arg 
    1100                 1105                 1110             


His Leu  Ala Gln Phe Asp Asn  Asn Pro Ala Val Ala  Phe Asp Thr 
    1115                 1120                 1125             


Lys Thr  Leu Lys Lys Met Pro  Leu Leu Lys Asn Gly  Glu Pro Leu 
    1130                 1135                 1140             


Lys Glu  Val Leu Cys Phe Glu  Glu Ile Phe Thr Ile  Arg Lys Asp 
    1145                 1150                 1155             


Ile Thr  Pro Asp Leu Lys Ile  Glu Lys Val Val Asp  Glu Lys Ile 
    1160                 1165                 1170             


Lys Ala  Ile Leu Glu Gln Arg  Lys Lys Asp Phe Gly  Gly Asn Ala 
    1175                 1180                 1185             


Lys Glu  Ala Phe Ser Asp Leu  Asp Lys Asn Pro Ile  Trp Leu Asn 
    1190                 1195                 1200             


Lys Lys  Lys Gly Ile Cys Ile  Lys Arg Val Thr Ile  Thr Gly Val 
    1205                 1210                 1215             


Ser Asn  Ala Glu Ala Leu His  His Gln Lys Asp His  Leu Gly Arg 
    1220                 1225                 1230             


Glu Ile  Leu Asp Asp Asn Gly  Asn Pro Ile Pro Ala  Asp Phe Val 
    1235                 1240                 1245             


Ser Thr  Gly Asn Asn His His  Leu Ala Val Tyr Leu  Asp Ser Glu 
    1250                 1255                 1260             


Gly Lys  Leu Gln Asp Asn Ile  Val Ser Phe Tyr Glu  Ala Val Val 
    1265                 1270                 1275             


Arg Thr  Ser Gln Gly Leu Pro  Val Val Asp Lys Gly  Leu Asn Lys 
    1280                 1285                 1290             


Asp Leu  Gly Trp Lys Phe Leu  Phe Ser Leu Lys Gln  Asn Glu Met 
    1295                 1300                 1305             


Phe Val  Phe Pro Asn Asp Asp  Phe Asp Ile Asn Asp  Phe Asp Leu 
    1310                 1315                 1320             


Leu Asp  Asp Lys Lys Ala Glu  Leu Ile Ser Lys His  Leu Tyr Arg 
    1325                 1330                 1335             


Val Gln  Lys Ile Ser Lys Val  Ser Ala Gly Asn Ser  Phe Val Arg 
    1340                 1345                 1350             


Asp Ile  Val Phe Arg His His  Leu Glu Thr Thr Val  Lys Glu Val 
    1355                 1360                 1365             


Lys Glu  Leu Lys Asp Ile Ser  Tyr Ile Gln Leu Lys  Ser Leu Glu 
    1370                 1375                 1380             


Gly Leu  Arg Asn Ile Lys Lys  Val Arg Ile Asn His  Ile Gly Lys 
    1385                 1390                 1395             


Ile Val  Gln Val Gly Glu Tyr  
    1400                 1405 


<210>  1192
<211>  1406
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1192

Met Glu Lys Lys Ile Ile Gly Ile Asp Leu Gly Val Thr Ser Ile Gly 
1               5                   10                  15      


Trp Ala Tyr Val Ile Glu Asp Glu Asn Gly His Ser Arg Ile Leu Arg 
            20                  25                  30          


Ile Gly Thr Arg Val Asn Pro Leu Thr Thr Glu Glu Leu Lys Asn Phe 
        35                  40                  45              


Glu Gln Gly Lys Pro Ile Thr Glu Thr Ala Glu Arg Thr Leu Lys Arg 
    50                  55                  60                  


Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Gln Arg Arg Thr Asn Leu 
65                  70                  75                  80  


Ile Asn Val Leu Leu Lys Asn Lys Leu Ile Thr Glu Thr Thr Ala Leu 
                85                  90                  95      


Ala Glu Asp Gly Lys Gly Thr Thr His Leu Thr His Ala Leu Arg Ala 
            100                 105                 110         


Lys Ala Ala Tyr Ala Gln Ile Pro Leu Glu Asp Phe Ala Arg Val Leu 
        115                 120                 125             


Leu Met Leu Asn Lys Lys Arg Gly Tyr Arg Ser Ser Arg Lys Val Lys 
    130                 135                 140                 


Thr Glu Ala Asp Gly Gln Ala Ile Asp Gly Met Ala Val Ala Lys Lys 
145                 150                 155                 160 


Leu Tyr Glu Glu Asn Ile Thr Pro Gly Gln Leu Cys Tyr Thr Leu Leu 
                165                 170                 175     


Leu Gln Gly Lys Lys Thr Leu Pro Asp Phe Tyr Arg Ser Asp Leu Gln 
            180                 185                 190         


Val Glu Met Glu Lys Val Trp Thr Phe Gln Lys Gln Phe His Gln Ala 
        195                 200                 205             


Ile Leu Thr Asp Glu Phe Arg Lys Tyr Ile Ala Gly Lys Gly Gln Arg 
    210                 215                 220                 


Ala Thr Ala Ala Ser Phe Trp Gly Lys Tyr Gly Phe Asn Thr Ala Glu 
225                 230                 235                 240 


Phe Lys Gly Ser Arg Glu Glu Lys Lys Leu Lys Ser Tyr Glu Trp Arg 
                245                 250                 255     


Ser Gln Ala Ile Thr Thr Pro Leu Ser Gln Glu Glu Val Ala Ala Val 
            260                 265                 270         


Ile Thr Asp Ile Asn Ser Asn Ile Ala Asn Ser Ser Gly Tyr Leu Gly 
        275                 280                 285             


Ala Ile Ser Asp Arg Ser Lys Glu Leu Phe Phe Asn Lys Gln Thr Val 
    290                 295                 300                 


Gly Gln Tyr Gln Tyr Asn Leu Leu Gln Gln Asn Pro His Thr Asn Leu 
305                 310                 315                 320 


Ser Gly Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Thr 
                325                 330                 335     


Ile Trp Glu Thr Gln Ala Thr Phe His Thr Met Leu Thr Pro Ser Leu 
            340                 345                 350         


Lys Glu Glu Ile Arg Asp Ile Val Ile Phe Tyr Gln Arg Lys Leu Lys 
        355                 360                 365             


Ser Gln Lys Lys Leu Val Ser Ile Cys Glu Phe Glu Lys Arg Ile Leu 
    370                 375                 380                 


Glu Pro Leu Gln Gly Asp Lys Val Val Gly Tyr Lys Val Ala Pro Lys 
385                 390                 395                 400 


Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Ala Leu Gly Arg 
                405                 410                 415     


Met Glu Ile Gln His Lys Val Thr Lys Glu Arg His Phe Leu Asp Leu 
            420                 425                 430         


Asp Ala Lys Gln Leu Leu Phe Asp Glu Leu Asn Val Lys Gly Asp Leu 
        435                 440                 445             


Thr Lys Glu Asn Ala Leu Thr Ile Leu Gly Phe Lys Pro Lys Glu Trp 
    450                 455                 460                 


Glu Cys Asn Leu Leu Lys Leu Glu Gly Asn Arg Thr Asn Gln Ala Leu 
465                 470                 475                 480 


Tyr Glu Ala Tyr Leu Lys Ile Leu Glu Leu Glu Gly Tyr Glu Val Lys 
                485                 490                 495     


Asp Leu Leu Gly Leu Lys Leu Asp Lys Asp Asp Ile Lys Leu Ser Glu 
            500                 505                 510         


Leu Pro Val Asp Ala Ala Asp Ile Lys Lys Met Val Arg Glu Ile Phe 
        515                 520                 525             


Glu Thr Leu Asn Ile Lys Thr Ser Leu Leu Asp Phe Asn Ala Thr Leu 
    530                 535                 540                 


Pro Gly Lys Ser Phe Glu Gln Gln His Ala Tyr Gln Leu Trp His Leu 
545                 550                 555                 560 


Leu Tyr Ser Tyr Glu Gly Asp Asn Ser Pro Ser Gly Asn Lys Lys Leu 
                565                 570                 575     


Gln Asp Leu Leu Gln Ala Lys Phe Gly Phe Thr Asn Ala His Gly Gln 
            580                 585                 590         


Ile Leu Ala Asn Val Ser Leu Glu Thr Asp Tyr Gly Ser Leu Ser Ala 
        595                 600                 605             


Lys Ala Ile Arg Lys Ile Phe Thr His Ile Lys Glu Asn His Tyr Ser 
    610                 615                 620                 


Ala Ala Cys Ala Leu Ala Gly Tyr Asn His Ser Ser Tyr Leu Ser Lys 
625                 630                 635                 640 


Glu Glu Asn Glu Gln Arg Ile Leu Lys Asp Arg Leu Thr Ile Leu Pro 
                645                 650                 655     


Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Leu 
            660                 665                 670         


Ile Asn Leu Leu Asn Thr Ile Phe Asp Asp Pro Tyr Leu Gly Lys Pro 
        675                 680                 685             


Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Val Gln 
    690                 695                 700                 


Glu Arg Gln Arg Leu Asn Thr Glu Ile Thr Lys Asn Asn Thr Glu His 
705                 710                 715                 720 


Glu Arg Ile Thr Lys Ile Leu Ile Lys Glu Asp Asn Ile Lys Asn Pro 
                725                 730                 735     


Thr Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Gln Glu Leu Lys His 
            740                 745                 750         


Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile Pro Arg Glu Glu 
        755                 760                 765             


Leu Phe Gly Lys Asn Phe Asp Ile Glu His Ile Ile Pro Gln Ser Ile 
    770                 775                 780                 


Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Cys Val Arg Lys Glu 
785                 790                 795                 800 


Asn Ile Asp Lys Ala Asp Arg Thr Ala Met Asp Phe Ile Gly Pro Tyr 
                805                 810                 815     


Phe Gly Glu Asp Lys Val Gln Glu Tyr Ile Asn Arg Val Glu Met Leu 
            820                 825                 830         


His Gln Ile Asn Leu Lys Asn Pro Glu Glu Gly Ile Gly Lys Gly Lys 
        835                 840                 845             


Tyr Lys Lys Leu Leu Met Lys Ala Ser Glu Ile Gly Gln Gly Phe Ile 
    850                 855                 860                 


Glu Arg Asp Leu Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Arg Gln 
865                 870                 875                 880 


Met Leu Leu Glu Val Thr Arg Thr Val Val Ala Thr Thr Gly Ser Ile 
                885                 890                 895     


Thr Lys Arg Leu Arg Glu Asp Trp Gly Leu Val Asn Val Met Gln Glu 
            900                 905                 910         


Leu Asn Met Asp Lys Tyr Arg Gln Gln Gly Leu Thr Glu Leu Ile Glu 
        915                 920                 925             


Lys Lys Asp Gly Ser Thr Lys Glu Arg Ile Thr Asp Trp Thr Lys Arg 
    930                 935                 940                 


Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe Thr 
945                 950                 955                 960 


Lys His Ser His Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Lys Ser 
                965                 970                 975     


Asp Asp Lys Lys Gly Lys Glu Ile Phe Gly Ile Glu Gln Lys Glu Thr 
            980                 985                 990         


Glu Lys Val Lys Asp Asp Thr Gly  Gln Thr Gln Arg Arg  Phe Lys Leu 
        995                 1000                 1005             


Pro Met  Pro Asp Phe Arg Gln  Ala Ala Lys His His  Leu Glu His 
    1010                 1015                 1020             


Leu Leu  Ile Ser Phe Lys Ala  Lys Asn Lys Val Phe  Thr Lys Asn 
    1025                 1030                 1035             


Lys Asn  Lys Tyr Lys Thr Lys  Asn Gly Glu Gln Val  Lys Leu Glu 
    1040                 1045                 1050             


Leu Thr  Pro Arg Gly Gln Leu  His Lys Glu Thr Ile  Tyr Gly Arg 
    1055                 1060                 1065             


Ile Lys  Lys Tyr Val Thr His  Glu Glu Lys Val Ser  Ser Lys Phe 
    1070                 1075                 1080             


Asn Ala  Gln Leu Ile Gln Thr  Val Ala Asn Ser Lys  Tyr Arg Tyr 
    1085                 1090                 1095             


Ala Leu  Leu Lys Arg Leu Glu  Glu Asn Gly Asn Asp  Pro Lys Lys 
    1100                 1105                 1110             


Ala Phe  Thr Gly Lys Asn Ser  Leu Asp Lys Thr Pro  Leu Phe Val 
    1115                 1120                 1125             


Asp Ala  Thr Gln Gln Glu Lys  Ile Pro Pro Val Val  Lys Leu Val 
    1130                 1135                 1140             


Ser Ile  Glu Gln Gly Tyr Thr  Ile Arg Lys Asp Ile  Thr Pro Glu 
    1145                 1150                 1155             


Asn Phe  Lys Asp Ala Lys Ser  Ile Glu Lys Ile Val  Asp Val Gly 
    1160                 1165                 1170             


Ile Arg  Asn Leu Leu His His  Arg Leu Glu Thr Tyr  Asn Gly Asn 
    1175                 1180                 1185             


Glu Lys  Glu Ala Phe Tyr Asn  Leu Asp Lys Asn Pro  Ile Trp Val 
    1190                 1195                 1200             


Asn Glu  Ser Lys Gly Ile Cys  Ile Lys Arg Val Thr  Ile Thr Gly 
    1205                 1210                 1215             


Val Thr  Asn Ala Glu Pro Leu  His Tyr Lys Arg Asp  His His Gly 
    1220                 1225                 1230             


Lys Pro  Ile Leu Asp Asn Asn  Gly Asn Arg Thr Pro  Ala Asp Phe 
    1235                 1240                 1245             


Val Ser  Thr Gly Asn Asn His  His Ala Ala Ile Tyr  Arg Asp Ala 
    1250                 1255                 1260             


Ala Gly  Asn Leu Gln Asp Asn  Val Val Ser Phe Tyr  Glu Ala Val 
    1265                 1270                 1275             


Ala Arg  Val Asn Gln Gly Leu  Pro Ile Ile Asp Lys  Ser Leu Asn 
    1280                 1285                 1290             


Lys Glu  Leu Gly Trp Gln Phe  Leu Phe Ser Leu Lys  Gln Asn Glu 
    1295                 1300                 1305             


Cys Phe  Val Phe Pro Asn Glu  Gly Phe Asp Pro Leu  Glu Ile Asp 
    1310                 1315                 1320             


Leu Tyr  Asn Pro Ala Asn Asn  Lys Leu Ile Ala Ala  Asn Leu Phe 
    1325                 1330                 1335             


Arg Val  Gln Asn Phe Ser Arg  Leu Gln Tyr Gly Asn  Asn Ile Ile 
    1340                 1345                 1350             


Arg Asp  Tyr Asn Phe Arg His  Ile Tyr Glu Thr Ser  Val Glu Asn 
    1355                 1360                 1365             


Arg Lys  Glu Leu Lys Asp Ile  Ala Tyr Lys Gln Val  Lys Ser Leu 
    1370                 1375                 1380             


Pro Ile  Phe Glu Lys Ile Ile  Lys Val Arg Ile Asn  His Ile Gly 
    1385                 1390                 1395             


Gln Ile  Val Lys Thr Gly Glu  Tyr 
    1400                 1405     


<210>  1193
<211>  1407
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1193

Met Ile Arg Asn Ile Leu Gly Leu Asp Leu Gly Val Ser Ser Ile Gly 
1               5                   10                  15      


Trp Ala Tyr Val Gln Glu Asp Ser Lys Asn Ser Glu Asn Asn Arg Ile 
            20                  25                  30          


Ile Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Gln Leu 
        35                  40                  45              


Asn Phe Glu Lys Gly Lys Pro Ile Thr Thr Asn Ala Gly Arg Thr Leu 
    50                  55                  60                  


Ala Arg Ser Ala Arg Arg Asn Leu Gln Arg Phe Lys Leu Arg Arg Ser 
65                  70                  75                  80  


Asn Leu Met Asp Val Leu Lys Lys Asn Asn Ile Leu Lys Glu Thr Asp 
                85                  90                  95      


Leu Leu Ala Glu Val Gly Lys Asn Ser Thr Phe Gln Thr Gln Glu Leu 
            100                 105                 110         


Arg Ala Lys Ser Ala Lys Glu Lys Ile Glu Leu Ser Glu Phe Ala Arg 
        115                 120                 125             


Val Leu Leu Leu Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys 
    130                 135                 140                 


Ala Lys Asn Asp Glu Asp Gly Gln Ile Val Asp Gly Met Ala Val Ala 
145                 150                 155                 160 


Lys Lys Leu Phe Glu Glu Asn Leu Thr Pro Gly Glu Tyr Ser Tyr Gln 
                165                 170                 175     


Leu Leu Gln Gln Gly Lys Lys Gln Leu Pro Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         


Leu Gln Ala Glu Phe Asp Lys Ile Trp Asp Phe Gln Lys Gln Phe Asn 
        195                 200                 205             


Pro Glu Ile Phe Thr Asn Glu Leu Tyr Glu Lys Leu Gln Gly Lys Asn 
    210                 215                 220                 


Arg Asn Ala Thr Trp Lys Glu Leu Glu Phe Pro Phe Ser Leu Val Gly 
225                 230                 235                 240 


Ile Lys Gln Thr Gly Thr Met Gln Glu Lys Lys Val Glu Lys Tyr Phe 
                245                 250                 255     


Trp Arg Ser Glu Ala Val Lys Lys Gln Leu Asp Phe Glu Ser Leu Ala 
            260                 265                 270         


Val Val Phe Gln Glu Ile Asn Ser Asn Leu Asn Asn Ser Ser Gly Tyr 
        275                 280                 285             


Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Tyr Asn Gln 
    290                 295                 300                 


Thr Val Gly Glu Tyr Leu Phe Gln Gln Leu Lys Thr Asn Pro His Thr 
305                 310                 315                 320 


Lys Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe 
                325                 330                 335     


Glu Lys Ile Trp Glu Thr Gln Ser Lys Tyr His Lys Glu Leu Thr Lys 
            340                 345                 350         


Glu Leu Lys Glu Glu Ile Arg Asp Ile Thr Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Gly Leu Ile Ser Ile Cys Glu Phe Glu Asn Arg 
    370                 375                 380                 


Glu Ile Glu Ile Thr Glu Asn Gly Lys Thr Lys Lys Lys Thr Val Gly 
385                 390                 395                 400 


Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile 
                405                 410                 415     


Trp Gln Val Leu Asn Asn Leu Gln Phe Gln Asn Ile Glu Thr Lys Glu 
            420                 425                 430         


Val Phe Pro Ile Asp Leu Asp Phe Lys Gln Ser Ile Phe Asp Glu Val 
        435                 440                 445             


Asn Ile Lys Gly Lys Leu Ser Ala Lys Asp Val Leu Asp Leu Val Gly 
    450                 455                 460                 


Tyr Ser Gly Lys Glu Trp Lys Thr Asn Phe Lys Glu Ile Glu Gly Asn 
465                 470                 475                 480 


Tyr Thr Asn Glu Asn Leu Tyr Thr Ala Phe Leu Lys Ile Ile Ala Ser 
                485                 490                 495     


Lys Glu Ile Glu Phe Pro Lys Glu Phe Lys Ile Thr Ile Asp Asp Glu 
            500                 505                 510         


Ile Lys Val Ser Lys Ile Asn Ala Ser Ala Glu Ile Ile Lys Leu Phe 
        515                 520                 525             


Val Arg Glu Lys Phe Ser Glu Leu Gly Ile Asp Thr Ser Ile Leu Asp 
    530                 535                 540                 


Phe Asn Pro Glu Leu Glu Gly Ser Asp Phe Glu Lys Gln Ala Ser Tyr 
545                 550                 555                 560 


Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp Ser Ser Ser 
                565                 570                 575     


Gly Asn Glu Lys Leu Tyr Glu Leu Leu Glu Asn Lys Phe Gly Phe Lys 
            580                 585                 590         


Lys Glu Asn Ser Lys Ile Leu Ala Glu Ile Gly Phe Ser Gln Asp Tyr 
        595                 600                 605             


Gly Ser Leu Ser Ser Lys Ala Met Arg Lys Ile Tyr Pro Tyr Ile Lys 
    610                 615                 620                 


Glu His Lys Tyr Ser Val Ala Cys Ser Tyr Ala Gly Tyr Asn His Ser 
625                 630                 635                 640 


Lys Asn Ser Leu Thr Lys Glu Gln Leu Glu Asn Arg Ile Leu Lys Glu 
                645                 650                 655     


Arg Leu Asp Ile Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
            660                 665                 670         


Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Cys Leu Ile Asp Glu 
        675                 680                 685             


Tyr Ser Glu Lys Asp Lys Asp Gly Gln Ile Val Lys Tyr Phe Lys Phe 
    690                 695                 700                 


Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Leu Asn Ala Glu 
705                 710                 715                 720 


Glu Arg Ala Thr Met Thr Ser Glu Ile Asn Lys Ala Thr Ile Gln His 
                725                 730                 735     


Gln Lys Tyr Ala Glu Val Leu Gln Arg Glu Phe Gly Leu Lys Ala Pro 
            740                 745                 750         


Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Leu Glu Leu Ala Asn 
        755                 760                 765             


Asn Gly Phe Lys Asp Leu Tyr Thr Asp Ala Lys Ile Glu Arg Glu Asn 
    770                 775                 780                 


Leu Phe Thr Asp Lys Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Arg 
785                 790                 795                 800 


Phe Phe Asp Asp Ser Phe Ser Asn Lys Val Leu Val Pro Arg Asn Ala 
                805                 810                 815     


Asn Leu Lys Lys Gly Asn Phe Thr Ala Phe Asp Tyr Leu Glu Ile Glu 
            820                 825                 830         


Gly Lys Glu Lys Leu Glu Lys Phe Leu Asn Ile Ile Lys Asp Leu Tyr 
        835                 840                 845             


Asp Lys Gly Ala Ile Thr Lys Ala Lys Tyr Glu Lys Leu Gln Lys Lys 
    850                 855                 860                 


Asp Phe Glu Ile Gly Asp Gly Phe Ile Glu Arg Asp Leu Arg Asn Thr 
865                 870                 875                 880 


Gln Tyr Ile Ala Lys Lys Ser Lys Glu Ile Leu Phe Gly Ile Thr Asn 
                885                 890                 895     


Ser Val Val Thr Thr Ser Gly Arg Ile Thr Asp Lys Leu Arg Glu Asp 
            900                 905                 910         


Trp Asn Leu Ile Asn Thr Met Lys Glu Leu Asn Leu Glu Lys Tyr Arg 
        915                 920                 925             


Lys Leu Gly Leu Thr Glu Thr Val Ile Asn Ser Lys Gly Glu Glu Lys 
    930                 935                 940                 


Gln Arg Ile Ile Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala 
945                 950                 955                 960 


Met Asp Ala Leu Thr Val Ala Phe Thr Thr His Asn His Ile Gln Tyr 
                965                 970                 975     


Leu Asn Tyr Leu Asn Ala Arg Lys Asp Glu Lys His Lys Glu His Gln 
            980                 985                 990         


Asn Ile Phe Ala Ile Glu Asn Leu  Ile Thr Glu Ile Ile  Asp Lys Lys 
        995                 1000                 1005             


Asn Gly  Ser Lys Glu Arg Arg  Phe Lys Glu Pro Val  Lys Asn Leu 
    1010                 1015                 1020             


Arg Ala  Glu Ala Arg Lys His  Leu Asp Glu Ile Leu  Ile Ser His 
    1025                 1030                 1035             


Lys Val  Lys Asn Lys Val Val  Thr Lys Asn Ile Asn  Lys Ile Lys 
    1040                 1045                 1050             


Lys Lys  Gly Ser Val Ile Ala  Lys Thr Glu Leu Thr  Pro Arg Gly 
    1055                 1060                 1065             


Gln Leu  His Lys Glu Thr Ile  Tyr Gly Ser Ser Lys  Phe Leu Lys 
    1070                 1075                 1080             


Thr Lys  Glu Glu Lys Ile Ser  Gly Lys Phe Asp Val  Glu Thr Ile 
    1085                 1090                 1095             


Gln Lys  Val Gln Asn Glu Arg  Tyr Arg Asn Ala Leu  Leu Lys Arg 
    1100                 1105                 1110             


Leu Gln  Glu Phe Gly Gly Asp  Ser Lys Lys Ala Phe  Thr Gly Lys 
    1115                 1120                 1125             


Asn Val  Ile Ser Lys Asn Pro  Ile Phe Leu Asn Asn  Glu Lys Thr 
    1130                 1135                 1140             


Glu Gln  Leu Pro Glu Thr Val  Thr Leu Ala Trp Tyr  Glu Met Gly 
    1145                 1150                 1155             


Tyr Thr  Ile Arg Lys Ala Val  Asn Pro Asp Asn Phe  Lys Asp Phe 
    1160                 1165                 1170             


Lys Asn  Ile Glu Lys Thr Ile  Asp Lys Gly Ile Arg  Asp Ile Leu 
    1175                 1180                 1185             


Thr Lys  Arg Leu Lys Glu Phe  Asn Gly Asn Ser Lys  Glu Ala Phe 
    1190                 1195                 1200             


Ser Asp  Leu Asp Lys Asn Pro  Ile Trp Leu Asn Lys  Ser Lys Gly 
    1205                 1210                 1215             


Ile Ser  Ile Lys Ala Val Thr  Ile Thr Gly Ile Asn  Asn Ala Glu 
    1220                 1225                 1230             


Ala Leu  His Tyr Lys Lys Asp  His Leu Gly Lys Asp  Phe Leu Asp 
    1235                 1240                 1245             


Glu Asn  Gly Lys Arg Ile Ala  Val Asp Phe Val Ser  Thr Gly Asn 
    1250                 1255                 1260             


Asn His  His Val Ala Ile Tyr  Glu Asp Glu Asn Gly  Asn Leu Gln 
    1265                 1270                 1275             


Glu Lys  Val Val Ser Phe Tyr  Glu Ala Val Glu Arg  Val Asn Gln 
    1280                 1285                 1290             


Asn Leu  Pro Ile Ile Asp Lys  Glu Tyr Asn Ser Glu  Ser Gly Trp 
    1295                 1300                 1305             


Lys Phe  Leu Phe Thr Met Lys  Gln Asn Glu Met Phe  Leu Phe Pro 
    1310                 1315                 1320             


Ser Glu  Asp Phe Asn Pro Lys  Glu Met Asp Leu Phe  Asp Glu Lys 
    1325                 1330                 1335             


Asn Val  Asn Leu Ile Ser Lys  Asn Leu Phe Arg Val  Gln Lys Phe 
    1340                 1345                 1350             


Thr Ile  Lys Asp Tyr Phe Phe  Arg His His Leu Glu  Thr Asn Val 
    1355                 1360                 1365             


Glu Asp  Ser Ser Pro Leu Lys  Gly Ile Thr Trp Lys  Arg Glu Gly 
    1370                 1375                 1380             


Leu Asn  Gly Ile Glu Asn Ile  Leu Lys Val Arg Leu  Asn His Leu 
    1385                 1390                 1395             


Gly Lys  Ile Val Gln Ile Gly  Glu Tyr 
    1400                 1405         


<210>  1194
<211>  1408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1194

Met Thr Lys Asn Ile Leu Gly Leu Asp Val Gly Val Ser Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Ile Asn Glu Asp Ile Glu Asn Pro Lys Asn Asn Lys Ile 
            20                  25                  30          


Ile Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Gln Ile 
        35                  40                  45              


Asn Phe Glu Lys Gly Lys Pro Ile Thr Thr Asn Ala Gly Arg Thr Leu 
    50                  55                  60                  


Ala Arg Gly Ala Arg Arg Asn Leu Gln Arg Phe Lys Leu Arg Arg Phe 
65                  70                  75                  80  


His Leu Ile Asp Ile Leu Gln Lys Asn Asn Ile Leu Lys Gln Ala Asp 
                85                  90                  95      


Leu Leu Ala Glu Val Gly Lys Asn Ser Thr Phe Gln Thr Gln Glu Ile 
            100                 105                 110         


Arg Ala Lys Ser Ala Lys Gln Lys Val Glu Leu Ser Glu Leu Ala Arg 
        115                 120                 125             


Ile Phe Leu Leu Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys 
    130                 135                 140                 


Ala Lys Asn Glu Asp Glu Gly Gln Ile Ile Asp Gly Met Phe Ile Ala 
145                 150                 155                 160 


Lys Lys Leu Tyr Asp Glu Lys Leu Thr Pro Gly Glu Tyr Ser Tyr Gln 
                165                 170                 175     


Leu Leu Lys Glu Ser Lys Lys Gln Leu Pro Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         


Leu Gln Ser Glu Phe Asp Lys Ile Trp Asn Phe Gln Lys Gln Phe Tyr 
        195                 200                 205             


Pro Glu Asn Phe Ser Asp Asn Leu Tyr Lys Glu Leu Gln Gly Lys Asn 
    210                 215                 220                 


Lys Asn Ala Thr Trp Lys Thr Leu Glu Ile Pro Phe Ser Leu Val Gly 
225                 230                 235                 240 


Ile Lys Gln Thr Gly Thr Leu Gln Glu Lys Lys Leu Glu Lys Tyr Ser 
                245                 250                 255     


Trp Arg Ser Asp Ala Val Lys Lys Gln Leu Asp Tyr Glu Lys Leu Ala 
            260                 265                 270         


Val Val Phe Gln Glu Ile Asn Ser Asn Ile Tyr Asn Ser Ser Gly Tyr 
        275                 280                 285             


Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Gln 
    290                 295                 300                 


Thr Val Gly Glu Tyr Leu Tyr Asn Gln Ile Lys Glu Asn Pro His Thr 
305                 310                 315                 320 


Lys Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe 
                325                 330                 335     


Glu Lys Ile Trp Glu Thr Gln Ser Lys Tyr His Thr Thr Leu Thr Lys 
            340                 345                 350         


Glu Leu Lys Glu Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Gly Leu Ile Ser Ile Cys Glu Phe Glu Asn Arg 
    370                 375                 380                 


Glu Ile Glu Ile Lys Glu Asn Gly Lys Ile Lys Lys Lys Thr Val Gly 
385                 390                 395                 400 


Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile 
                405                 410                 415     


Trp Gln Ile Leu Asn Asn Leu Gln Phe Gln Asn Ile Glu Ser Lys Glu 
            420                 425                 430         


Ile Phe Pro Ile Asp Leu Asp Phe Lys Glu Ser Ile Phe Asn Glu Val 
        435                 440                 445             


Asn Ile Lys Gly Arg Ile Ser Ala Lys Ala Val Leu Asp Leu Leu Gly 
    450                 455                 460                 


Tyr Ser Gly Lys Glu Trp Asn Pro Asn Phe Lys Asp Ile Glu Gly Asn 
465                 470                 475                 480 


Asn Thr Asn Glu Asn Leu Tyr Asn Ala Tyr Leu Lys Ile Ile Ala Asp 
                485                 490                 495     


Glu Glu Phe Glu Leu Pro Lys Glu Phe Lys Leu Asn Gly Asp Asp Asp 
            500                 505                 510         


Leu Tyr Val Ser Lys Ile Ala Cys Ser Ala Ser Lys Ile Lys Asp Phe 
        515                 520                 525             


Val Lys Glu Lys Phe Ser Glu Ile Gly Ile Asn Thr Ser Ile Leu Asp 
    530                 535                 540                 


Phe Asn Pro Glu Leu Glu Gly Lys Asp Phe Glu Lys Gln Ala Ser Tyr 
545                 550                 555                 560 


Gln Phe Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp Ser Gln Ser 
                565                 570                 575     


Gly Asn Glu Lys Leu Tyr Glu Leu Leu Glu Lys Lys Phe Gly Phe Lys 
            580                 585                 590         


Lys Glu His Ser Lys Ile Leu Ser Asn Ile Gly Phe Pro Leu Asp Tyr 
        595                 600                 605             


Gly Asn Leu Ser Thr Lys Ala Met Arg Lys Ile Ile Pro His Leu Lys 
    610                 615                 620                 


Ala Gly Leu Ser Phe Ala Lys Lys Ser Lys Glu Asp Asn Ile Leu Ser 
625                 630                 635                 640 


Ala Thr Ala Met Ala Gly Tyr Asn His Ser Asn Ser Tyr Thr Lys Glu 
                645                 650                 655     


Asp Leu Ala Lys Arg Val Leu Lys Glu Lys Leu Glu Val Phe Pro Lys 
            660                 665                 670         


Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Met Ile 
        675                 680                 685             


Asn Leu Val Asn Glu Ala Val Glu Glu Tyr Gly Thr Ile Asp Glu Val 
    690                 695                 700                 


Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Glu Glu Arg Ala 
705                 710                 715                 720 


Asn Met Thr Ser Glu Ile Gly Lys Ala Thr Leu Leu His Gln Lys Tyr 
                725                 730                 735     


Ala Glu Ile Leu Lys Lys Glu Phe Asp Ile Pro Val Pro Ser Arg Asn 
            740                 745                 750         


Asp Ile Ile Arg Tyr Lys Leu Tyr Leu Glu Leu Ala Asn Asn Gly Tyr 
        755                 760                 765             


Lys Asp Leu Tyr Thr Asp Val Gln Ile Lys Arg Glu Glu Leu Phe Thr 
    770                 775                 780                 


Asn Lys Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Arg Phe Phe Asp 
785                 790                 795                 800 


Asp Ser Phe Ser Asn Lys Val Leu Val Pro Arg Gly Ala Asn Leu Lys 
                805                 810                 815     


Lys Ala Asn Gln Thr Ala Phe Asp Tyr Leu Glu Asn Glu Asp Lys Gln 
            820                 825                 830         


Lys Leu Glu Lys Phe Leu Asn Ile Ile Lys Asn Leu Tyr Asp Lys Gly 
        835                 840                 845             


Glu Ile Thr Lys Ala Lys Tyr Glu Lys Leu Gln Lys Lys Gly Ser Asp 
    850                 855                 860                 


Ile Gly Glu Gly Phe Ile Glu Arg Asp Leu Arg Asp Thr Gln Tyr Ile 
865                 870                 875                 880 


Ala Lys Lys Ala Lys Glu Ile Leu Phe Gly Ile Thr His Ser Val Ile 
                885                 890                 895     


Ser Thr Ser Gly Lys Ile Thr Asp Lys Leu Arg Glu Asp Trp Asn Leu 
            900                 905                 910         


Val Asn Thr Met Lys Glu Leu Asn Leu Asp Lys Tyr Lys Lys Leu Gly 
        915                 920                 925             


Leu Thr Glu Thr Leu Leu Asn Ser Lys Gly Glu Glu Lys Glu Arg Ile 
    930                 935                 940                 


Ile Asp Trp Ser Lys Arg Asn Asp His Arg His His Ala Met Asp Ala 
945                 950                 955                 960 


Leu Thr Val Ala Phe Thr Thr His Asn His Ile Gln Tyr Leu Asn Tyr 
                965                 970                 975     


Leu Asn Ala Arg Lys Asp Glu Asp His Lys Gln His Lys Val Ile Ser 
            980                 985                 990         


Asn Ile Glu Asn Leu Ile Thr Lys  Val Phe Glu Lys Lys  Asn Gly Ala 
        995                 1000                 1005             


Lys Gln  Arg Arg Phe Ile Glu  Pro Ile Asn Asn Phe  Arg Val Asp 
    1010                 1015                 1020             


Ala Lys  Lys His Leu Asp Glu  Ile Leu Ile Ser His  Lys Ala Lys 
    1025                 1030                 1035             


Asn Lys  Val Val Thr Lys Asn  Ile Asn Lys Ile Lys  Lys Lys Gly 
    1040                 1045                 1050             


Ser Val  Val Ala Lys Thr Glu  Leu Thr Pro Arg Gly  Gln Leu His 
    1055                 1060                 1065             


Lys Glu  Thr Ile Tyr Gly Ser  Ser Lys Phe Leu Lys  Thr Lys Asp 
    1070                 1075                 1080             


Glu Lys  Ile Ser Gly Lys Phe  Asp Leu Glu Thr Ile  Asn Lys Val 
    1085                 1090                 1095             


Gln Asn  Glu Lys Tyr Lys Asn  Ala Leu Leu Gln Arg  Leu Arg Glu 
    1100                 1105                 1110             


Phe Gly  Glu Asp Ser Lys Lys  Ala Phe Thr Gly Lys  Asn Val Ile 
    1115                 1120                 1125             


Ser Lys  Asn Pro Ile Tyr Leu  Asp Glu Glu Lys Thr  Leu Gln Ile 
    1130                 1135                 1140             


Pro Glu  Ser Val Thr Leu Ala  Trp Tyr Glu Met Ile  Tyr Thr Ile 
    1145                 1150                 1155             


Arg Lys  Pro Val Asn Pro Asp  Asn Phe Lys Asp Tyr  Lys Asn Ile 
    1160                 1165                 1170             


Asp Lys  Val Thr Asp Glu Lys  Ile Arg Ala Val Leu  Thr Asp Arg 
    1175                 1180                 1185             


Leu Arg  Lys Phe Asn Gly Asn  Ser Lys Glu Ala Phe  Ser Asp Leu 
    1190                 1195                 1200             


Glu Lys  Asn Pro Ile Trp Leu  Asn Lys Ser Lys Gly  Ile Ala Ile 
    1205                 1210                 1215             


Lys Thr  Val Thr Ile Thr Gly  Met Ser Asn Val Glu  Ala Leu His 
    1220                 1225                 1230             


Tyr Lys  Lys Asp His Leu Gly  Lys Glu Ile Leu Asp  Lys Lys Gly 
    1235                 1240                 1245             


Asp Arg  Met Pro Val Asp Phe  Val Ser Thr Gly Asn  Asn His His 
    1250                 1255                 1260             


Ala Ala  Ile Tyr Glu Asp Ala  Lys Gly Asn Leu Gln  Glu Arg Val 
    1265                 1270                 1275             


Val Ser  Phe Tyr Glu Ala Val  Glu Arg Val Asn Gln  Gly Leu Ser 
    1280                 1285                 1290             


Val Val  Asn Lys Asp Tyr Asn  Ala Gly Leu Gly Trp  Lys Phe Leu 
    1295                 1300                 1305             


Phe Thr  Met Lys Gln Asn Glu  Met Phe Leu Phe Pro  Ser Glu Asp 
    1310                 1315                 1320             


Phe Asn  Pro Lys Glu Ile Asp  Leu Tyr Asp Glu Lys  Asn Leu Lys 
    1325                 1330                 1335             


Leu Ile  Ala Lys Asn Leu Phe  Arg Val Gln Lys Phe  Gly Glu Leu 
    1340                 1345                 1350             


Ser Lys  Ser Gly Phe Trp Phe  Arg His His Leu Glu  Thr Ser Val 
    1355                 1360                 1365             


Asp Val  Lys Lys Glu Leu Arg  Asn Tyr Ser Tyr Leu  Asp Phe Tyr 
    1370                 1375                 1380             


Ser Lys  Asp Phe Met Lys Thr  Ile Val Lys Val Arg  Leu Asn His 
    1385                 1390                 1395             


Leu Gly  Lys Ile Val Gln Val  Gly Glu Tyr 
    1400                 1405             


<210>  1195
<211>  1409
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1195

Met Ser Ile Ile Asn Phe Gln Arg Arg Gly Leu Met Glu Thr Gln Ala 
1               5                   10                  15      


Ser Asn Gln Leu Ile Ser Ser His Leu Lys Gly Tyr Pro Ile Lys Asp 
            20                  25                  30          


Tyr Phe Val Gly Leu Asp Ile Gly Thr Ser Ser Val Gly Trp Ala Val 
        35                  40                  45              


Thr Asn Lys Ala Tyr Glu Leu Leu Lys Phe Arg Ser His Lys Met Trp 
    50                  55                  60                  


Gly Ser Arg Leu Phe Asp Glu Gly Glu Ser Ala Val Ala Arg Arg Gly 
65                  70                  75                  80  


Phe Arg Ser Met Arg Arg Arg Leu Glu Arg Arg Lys Leu Arg Leu Lys 
                85                  90                  95      


Leu Leu Glu Glu Leu Phe Ala Asp Ala Met Ala Gln Val Asp Pro Thr 
            100                 105                 110         


Phe Phe Met Arg Leu Arg Glu Ser Lys Tyr His Tyr Glu Asp Lys Thr 
        115                 120                 125             


Thr Gly His Ser Ser Lys His Ile Leu Phe Ile Asp Lys Asn Tyr Thr 
    130                 135                 140                 


Asp Gln Asp Tyr Tyr Lys Ala Tyr Pro Thr Ile Tyr His Leu Arg Ala 
145                 150                 155                 160 


Glu Leu Met Lys Asp Gly Thr Asp Asp Ile Arg Lys Leu Phe Leu Ala 
                165                 170                 175     


Val His His Ile Leu Lys Tyr Arg Gly Asn Phe Leu Tyr Glu Gly Ala 
            180                 185                 190         


Thr Phe Asp Ser Asn Ala Ser Thr Leu Asp Asp Val Ile Lys Gln Ala 
        195                 200                 205             


Leu Glu Asn Ile Thr Phe Asn Cys Phe Asp Cys Asn Ser Ala Ile Ser 
    210                 215                 220                 


Ser Ile Gly Gln Ile Leu Met Glu Ala Gly Lys Thr Lys Ser Asp Lys 
225                 230                 235                 240 


Ala Lys Ala Ile Glu His Leu Val Asp Thr Tyr Ile Ala Thr Asp Thr 
                245                 250                 255     


Val Asp Thr Ser Ser Lys Thr Gln Lys Asp Gln Val Lys Glu Asp Lys 
            260                 265                 270         


Lys Arg Leu Lys Ala Phe Ala Asn Leu Val Leu Gly Leu Asn Ala Ser 
        275                 280                 285             


Leu Ile Asp Leu Phe Gly Ser Val Glu Glu Leu Glu Glu Asp Leu Lys 
    290                 295                 300                 


Lys Leu Gln Ile Thr Gly Asp Thr Tyr Asp Asp Lys Arg Asp Glu Leu 
305                 310                 315                 320 


Ala Lys Ala Trp Ser Asp Glu Ile Tyr Ile Ile Asp Asp Cys Lys Ser 
                325                 330                 335     


Val Tyr Asp Ala Ile Ile Leu Leu Ser Ile Lys Glu Pro Gly Leu Thr 
            340                 345                 350         


Ile Ser Glu Ser Lys Val Lys Ala Phe Asn Lys His Lys Asp Asp Leu 
        355                 360                 365             


Ala Ile Leu Lys Ser Leu Leu Lys Ser Asp Arg Ser Ile Tyr Asn Thr 
    370                 375                 380                 


Met Phe Lys Val Asp Glu Lys Gly Leu His Asn Tyr Val His Tyr Ile 
385                 390                 395                 400 


Lys Gln Gly Arg Thr Glu Glu Thr Ser Cys Asn Arg Glu Asp Phe Tyr 
                405                 410                 415     


Lys Tyr Thr Lys Lys Val Val Glu Gly Leu Pro Asp Ser Lys Asp Lys 
            420                 425                 430         


Glu Tyr Ile Leu Ser Glu Ile Glu Leu Gln Thr Leu Leu Pro Leu Gln 
        435                 440                 445             


Arg Ile Lys Asp Asn Gly Val Ile Pro Tyr Gln Leu His Leu Glu Glu 
    450                 455                 460                 


Leu Lys Val Ile Leu Ala Lys Cys Gly Pro Lys Phe Pro Phe Leu Asn 
465                 470                 475                 480 


Glu Val Ala Asp Gly Phe Ser Val Ala Glu Lys Leu Ile Lys Met Leu 
                485                 490                 495     


Glu Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Thr His His Asn 
            500                 505                 510         


Val Asp Asn Gly Gly Phe Ala Trp Ala Val Arg Lys Ala Ser Gly Arg 
        515                 520                 525             


Val Thr Pro Trp Asn Phe Asp Asp Lys Ile Asp Arg Glu Lys Ser Ala 
    530                 535                 540                 


Ala Ala Phe Ile Lys Asn Leu Thr Asn Lys Cys Thr Tyr Leu Leu Gly 
545                 550                 555                 560 


Glu Asp Val Leu Pro Lys Ser Ser Leu Leu Tyr Ser Glu Phe Met Leu 
                565                 570                 575     


Leu Asn Glu Leu Asn Asn Val Arg Ile Asp Gly Lys Pro Leu Glu Lys 
            580                 585                 590         


Ala Val Lys Glu His Leu Ile Glu Ala Val Phe Lys Gln Asp His Lys 
        595                 600                 605             


Lys Met Thr Lys Asn Arg Ile Glu Gln Phe Leu Lys Asp Asn Asn Tyr 
    610                 615                 620                 


Ile Pro Glu Lys His Lys Pro Glu Ile Thr Gly Leu Asp Gly Glu Ile 
625                 630                 635                 640 


Lys Asn Asp Leu Ala Ser Tyr Arg Asp Met Val Arg Ile Leu Gly Asn 
                645                 650                 655     


Asn Phe Asp Leu Ser Met Ala Glu Glu Ile Ile Thr Asp Ile Thr Ile 
            660                 665                 670         


Phe Gly Glu Ser Lys Lys Met Leu Arg Glu Thr Leu Arg Asn Lys Phe 
        675                 680                 685             


Ser Ser Arg Leu Asp Asp Glu Ala Ile Lys Lys Leu Ser Lys Leu Arg 
    690                 695                 700                 


Tyr Arg Asp Trp Gly Arg Leu Ser Arg Lys Leu Leu Asn Val Ile Glu 
705                 710                 715                 720 


Gly Cys Asp Lys Ala Gly Asp Gly Thr Pro Glu Thr Ile Ile Ile Leu 
                725                 730                 735     


Met Arg Asn Phe Ser Tyr Asn Leu Met Glu Leu Leu Gly Asp Lys Phe 
            740                 745                 750         


Ser Phe Met Glu Arg Ile Gln Glu Ile Asn Ala Lys Leu Ile Glu Gly 
        755                 760                 765             


Gln Ile Val Asn Pro His Asp Ile Ile Asp Asp Leu Ala Leu Ser Pro 
    770                 775                 780                 


Ala Val Lys Arg Ala Val Trp Gln Ala Leu Arg Ile Val Asp Glu Ile 
785                 790                 795                 800 


Val His Ile Lys Lys Thr Leu Pro Ala Arg Ile Phe Val Glu Val Thr 
                805                 810                 815     


Arg Ser Asn Lys Ala Glu Lys Lys Lys Lys Asp Ser Arg Gln Lys Arg 
            820                 825                 830         


Leu Ser Asp Leu Tyr Ala Ala Ile Lys Lys Asp Asp Ala Leu Leu Ser 
        835                 840                 845             


Gly Leu Thr Asn Glu Glu Phe Gly Gly Leu Lys Ser Gly Leu Ala Lys 
    850                 855                 860                 


Tyr Asp Asp Ala Ala Leu Arg Ser Lys Lys Leu Tyr Leu Tyr Tyr Thr 
865                 870                 875                 880 


Gln Met Gly Arg Cys Ala Tyr Thr Gly Glu Val Ile Asp Leu Ser Gln 
                885                 890                 895     


Leu Asn Thr Asp Asn Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Leu 
            900                 905                 910         


Thr Lys Asp Asp Ser Phe Asp Asn Leu Val Leu Cys Lys Arg Thr Ala 
        915                 920                 925             


Asn Ala Gln Lys Ser Asp Thr Tyr Pro Ile Ser Lys Glu Ile Gln Lys 
    930                 935                 940                 


Ala Gln Lys Ala Phe Trp Ile Phe Leu Lys Gln Gln Gly Leu Ile Ser 
945                 950                 955                 960 


Glu Arg Lys Tyr Glu Arg Leu Thr Arg Ile Thr Pro Leu Thr Ala Asp 
                965                 970                 975     


Asp Leu Ser Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Asn Gln Ser 
            980                 985                 990         


Val Lys Ala Ala Thr Thr Leu Leu  Arg Arg Leu Tyr Pro  Gly Val Asp 
        995                 1000                 1005             


Val Val  Phe Val Lys Ala Glu  Asn Val Thr Asp Phe  Arg His Asp 
    1010                 1015                 1020             


Asn Asp  Phe Ile Lys Val Arg  Ser Leu Asn His His  His His Ala 
    1025                 1030                 1035             


Lys Asp  Ala Tyr Leu Asn Ile  Val Val Gly Asn Val  Tyr His Glu 
    1040                 1045                 1050             


Arg Phe  Thr Arg Asn Phe Arg  Ala Phe Phe Lys Lys  Asn Gly Ala 
    1055                 1060                 1065             


Asn Arg  Thr Tyr Asn Leu Ala  Lys Met Phe Asn Tyr  Asp Val Asn 
    1070                 1075                 1080             


Cys Thr  Asn Ala Lys Asp Gly  Lys Ala Trp Asp Val  Lys Thr Ser 
    1085                 1090                 1095             


Met Asp  Thr Val Lys Lys Met  Met Ala Ser Asn Asp  Val Arg Val 
    1100                 1105                 1110             


Thr Lys  Arg Leu Leu Glu Gln  Ser Gly Ala Leu Ala  Asp Ala Thr 
    1115                 1120                 1125             


Val Tyr  Lys Ala Ala Val Ala  Glu Lys Ala Lys Asp  Gly Ala Tyr 
    1130                 1135                 1140             


Ile Gly  Met Lys Thr Lys Ser  Ser Val Phe Ala Asp  Val Ser Lys 
    1145                 1150                 1155             


Tyr Gly  Gly Met Thr Lys Ile  Lys Asn Ala Tyr Ser  Ile Ile Val 
    1160                 1165                 1170             


Gln Tyr  Ala Gly Lys Lys Gly  Glu Val Val Lys Glu  Ile Val Pro 
    1175                 1180                 1185             


Leu Pro  Ile Tyr Leu Thr Asn  Arg Asn Thr Thr Asp  Gln Asp Leu 
    1190                 1195                 1200             


Ile Asp  Tyr Val Ala Ser Ile  Ile Pro Gln Ala Lys  Asp Ile Ser 
    1205                 1210                 1215             


Ile Ile  Tyr Arg Lys Leu Cys  Ile Asn Gln Leu Val  Lys Val Asn 
    1220                 1225                 1230             


Gly Phe  Tyr Tyr Tyr Leu Gly  Gly Lys Thr Asn Ser  Lys Phe Cys 
    1235                 1240                 1245             


Ile Asp  Asn Ala Ile Gln Val  Ile Val Ser Asn Glu  Trp Ile Pro 
    1250                 1255                 1260             


Tyr Leu  Lys Val Leu Glu Lys  Phe Asn Asn Met Arg  Lys Asp Asn 
    1265                 1270                 1275             


Lys Asp  Leu Lys Ala Asn Val  Ile Ser Thr Arg Ala  Leu Asp Asn 
    1280                 1285                 1290             


Lys His  Thr Ile Glu Val Arg  Ile Val Glu Glu Lys  Asn Ile Glu 
    1295                 1300                 1305             


Phe Phe  Asp Tyr Leu Val Ser  Lys Leu Lys Met Pro  Ile Tyr Gln 
    1310                 1315                 1320             


Lys Met  Lys Gly Asn Lys Ala  Ala Glu Leu Ser Glu  Lys Gly Tyr 
    1325                 1330                 1335             


Gly Leu  Phe Lys Lys Met Ser  Leu Glu Glu Gln Ser  Ile His Leu 
    1340                 1345                 1350             


Ile Glu  Leu Leu Asn Leu Leu  Thr Asn Gln Lys Thr  Thr Phe Glu 
    1355                 1360                 1365             


Val Lys  Pro Leu Gly Ile Thr  Ala Ser Arg Ser Thr  Val Gly Ser 
    1370                 1375                 1380             


Lys Ile  Ser Asn Gln Asp Glu  Phe Lys Val Ile Asn  Glu Ser Ile 
    1385                 1390                 1395             


Thr Gly  Leu Tyr Ser Asn Glu  Val Thr Ile Val 
    1400                 1405                 


<210>  1196
<211>  1410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1196

Met Thr Lys Asn Ile Leu Gly Leu Asp Leu Gly Val Ser Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Val Gln Glu Asn Ser Lys Asn Pro Glu Asn Asn Lys Ile 
            20                  25                  30          


Val Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Gln Ile 
        35                  40                  45              


Asn Phe Glu Lys Gly Lys Pro Ile Thr Thr Asn Ala Ser Arg Thr Leu 
    50                  55                  60                  


Ala Arg Ser Ala Arg Arg Asn Leu Gln Arg Phe Lys Leu Arg Arg Ala 
65                  70                  75                  80  


Asn Leu Ile Asp Val Leu Lys Lys Gly Asn Ile Leu Lys Gln Gly Asp 
                85                  90                  95      


Leu Leu Thr Glu Ile Gly Lys Asn Ser Thr Phe Gln Thr Gln Glu Leu 
            100                 105                 110         


Arg Ala Lys Ser Ala Lys Glu Lys Val Glu Leu Ser Asp Phe Val Arg 
        115                 120                 125             


Ile Leu Leu Leu Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys 
    130                 135                 140                 


Ala Lys Asn Glu Asp Glu Gly Gln Ile Ile Asp Gly Met Ala Val Ala 
145                 150                 155                 160 


Lys Lys Leu Tyr Glu Glu Asn Ile Thr Pro Gly Glu Tyr Ser Tyr Lys 
                165                 170                 175     


Leu Leu Gln Glu Gly Arg Lys Gln Leu Pro Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         


Leu Gln Ala Glu Phe Asp Lys Ile Trp Asn Phe Gln Lys Gln Phe Asn 
        195                 200                 205             


Pro Glu Ile Phe Thr Asn Glu Leu Tyr Lys Arg Leu Gln Gly Lys Asn 
    210                 215                 220                 


Arg Asn Ala Thr Trp Lys Glu Leu Glu Ile Pro Phe Tyr Leu Val Gly 
225                 230                 235                 240 


Ile Lys Gln Ile Gly Thr Met Gln Glu Lys Lys Ile Glu Arg Tyr Phe 
                245                 250                 255     


Trp Arg Ser Glu Ala Val Lys Lys Gln Leu Asp Phe Glu Ser Leu Ala 
            260                 265                 270         


Val Val Phe Gln Glu Ile Asn Ser Asn Leu Asn Asn Ser Ser Gly Tyr 
        275                 280                 285             


Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Gln 
    290                 295                 300                 


Thr Val Gly Glu Tyr Leu Tyr Asn Gln Leu Lys Ile Asn Pro His Thr 
305                 310                 315                 320 


Lys Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe 
                325                 330                 335     


Glu Lys Ile Trp Glu Thr Gln Ala Lys Tyr His Lys Lys Leu Thr Lys 
            340                 345                 350         


Glu Leu Lys Glu Glu Ile Arg Asp Ile Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Asn Arg 
    370                 375                 380                 


Glu Ile Glu Ile Thr Val Asn Gly Lys Thr Lys Lys Lys Thr Val Gly 
385                 390                 395                 400 


Leu Lys Val Val Pro Arg Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile 
                405                 410                 415     


Trp Gln Arg Leu Asn Asp Leu Glu Phe Lys Asn Val Glu Thr Lys Glu 
            420                 425                 430         


Ile Phe Pro Leu Glu Leu Glu His Lys Glu Ile Leu Phe Gly Asn Leu 
        435                 440                 445             


Asn Ile Lys Gly Asn Leu Ser Ser Lys Glu Leu Ile Glu Gln Leu Gly 
    450                 455                 460                 


Tyr Pro Ser Lys Glu Trp Lys Cys Asn Phe Lys Glu Ile Asp Gly Asn 
465                 470                 475                 480 


Lys Thr His Glu Asn Leu Tyr Thr Ala Phe Leu Lys Ile Ile Ala Asn 
                485                 490                 495     


Glu Gly Ile Glu Phe Pro Lys Glu Phe Lys Leu Ser Ile Asp Asp Asp 
            500                 505                 510         


Ile Lys Val Ser Lys Ile Asp Val Ser Ser Glu Lys Ile Lys Glu Phe 
        515                 520                 525             


Ile Lys Glu Asn Phe Leu Lys Leu Gly Ile Asn Thr Ser Ile Leu Asp 
    530                 535                 540                 


Phe Asn Pro Glu Leu Asp Gly Lys Asp Phe Glu Lys Gln Glu Ser Tyr 
545                 550                 555                 560 


Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp Ser Ala Ser 
                565                 570                 575     


Gly Asn Glu Lys Leu Tyr Glu Leu Leu Glu Lys Lys Phe Gly Phe Lys 
            580                 585                 590         


Arg Glu Tyr Ser Lys Ile Leu Ala Asp Val Gly Phe Ser Gln Asp Tyr 
        595                 600                 605             


Gly Ser Leu Ser Ser Lys Ala Met Arg Lys Ile Phe Thr Tyr Ile Lys 
    610                 615                 620                 


Glu Asn Lys Tyr Ser Glu Ala Cys His Leu Ala Gly Tyr Asn His Ser 
625                 630                 635                 640 


Lys Asn Ser Leu Thr Lys Glu Gln Leu Ala Asn Arg Ile Leu Lys Glu 
                645                 650                 655     


Gln Leu Glu Ile Leu Pro Lys Asn Ala Leu Arg Asn Pro Val Val Glu 
            660                 665                 670         


Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Glu Val Ile Lys Glu 
        675                 680                 685             


Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys 
    690                 695                 700                 


Lys Asn Ala Glu Glu Arg Ala Asn Met Thr Ser Asp Ile Ser Lys Ala 
705                 710                 715                 720 


Thr Leu Leu His Gln Lys Tyr Ala Glu Val Leu Gln Lys Glu Phe Gly 
                725                 730                 735     


Ile Lys Ala Pro Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Leu 
            740                 745                 750         


Glu Leu Ala Asn Asn Gly Tyr Lys Asp Leu Tyr Thr Asn Gln Lys Ile 
        755                 760                 765             


Glu Lys Glu Asn Ile Phe Thr Asp Lys Tyr Asp Ile Asp His Ile Ile 
    770                 775                 780                 


Pro Gln Ser Arg Phe Phe Asp Asp Ser Phe Ser Asn Lys Val Leu Val 
785                 790                 795                 800 


Pro Arg Cys Ala Asn Gln Asp Lys Asp Asn Tyr Thr Ala Val Asp Tyr 
                805                 810                 815     


Ile Glu Lys Lys Tyr Gly Glu Arg Gly Lys Glu Asp Phe Leu Asn Arg 
            820                 825                 830         


Ile His Leu Val Phe Lys Ser Lys Glu Ala Ile Ala Gly Lys Ser Glu 
        835                 840                 845             


Ser Gln Ile Ser Ser Met Asn Thr Lys Phe Leu Lys Leu Gln Lys Lys 
    850                 855                 860                 


Gly Ser Glu Ile Gly Glu Gly Phe Ile Glu Arg Asp Leu Arg Asp Thr 
865                 870                 875                 880 


Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Phe Gly Ile Thr Asn 
                885                 890                 895     


Ser Val Ile Ser Thr Ser Gly Arg Ile Thr Asp Lys Leu Arg Glu Asp 
            900                 905                 910         


Trp Asn Leu Val Asn Thr Met Lys Glu Leu Asn Leu Glu Lys Tyr Arg 
        915                 920                 925             


Lys Leu Gly Leu Thr Glu Leu Ile Ala Asn Ser Lys Gly Glu Glu Lys 
    930                 935                 940                 


Glu Arg Ile Ile Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala 
945                 950                 955                 960 


Met Asp Ala Leu Thr Val Ala Phe Thr Thr His Asn His Ile Gln Tyr 
                965                 970                 975     


Leu Asn Tyr Leu Asn Ala Arg Lys Asp Glu Lys His Lys Glu His His 
            980                 985                 990         


Asn Ile Phe Ala Ile Glu Asn Ile  Ile Thr Glu Ile Val  Glu Lys Lys 
        995                 1000                 1005             


Asn Gly  Ser Lys Glu Arg Arg  Phe Lys Leu Pro Ile  Lys Asn Phe 
    1010                 1015                 1020             


Arg Thr  Glu Ala Lys Lys Gln  Leu Glu Glu Val Leu  Ile Ser His 
    1025                 1030                 1035             


Lys Ala  Lys Asn Lys Val Val  Thr Lys Asn Ile Asn  Lys Thr Lys 
    1040                 1045                 1050             


Lys Lys  Gly Gly Val Val Ser  Lys Ile Val Leu Thr  Pro Arg Gly 
    1055                 1060                 1065             


Gln Leu  His Lys Glu Thr Ile  Tyr Gly Ser Ala Lys  Phe Leu Lys 
    1070                 1075                 1080             


Thr Lys  Glu Glu Lys Ile Ser  Gly Lys Phe Asp Leu  Glu Thr Ile 
    1085                 1090                 1095             


Asn Lys  Val Gln Asn Glu Lys  Phe Lys Asn Ala Leu  Leu Asp Arg 
    1100                 1105                 1110             


Leu Lys  Glu Phe Asn Gly Asp  Ser Lys Lys Ala Phe  Thr Gly Lys 
    1115                 1120                 1125             


Asn Val  Pro Ala Lys Asn Pro  Ile Tyr Leu Asn Glu  Gln Lys Thr 
    1130                 1135                 1140             


Glu Gln  Val Pro Glu Ser Val  Val Leu Ala Trp Tyr  Glu Thr Gly 
    1145                 1150                 1155             


Tyr Thr  Ile Arg Lys Ala Val  Asn Ala Asp Asn Phe  Lys Asp Tyr 
    1160                 1165                 1170             


Lys Asn  Leu Glu Lys Ile Ile  Asp Lys Gly Val Lys  Glu Ile Leu 
    1175                 1180                 1185             


Lys Asn  Arg Leu Asn Ala Phe  Asn Gly Asn Ala Lys  Glu Ala Phe 
    1190                 1195                 1200             


Ser Asp  Leu Glu Lys Lys Pro  Ile Trp Leu Asn Gln  Ser Lys Gly 
    1205                 1210                 1215             


Ile Ala  Val Lys Thr Val Thr  Ile Thr Gly Ile Asn  Asn Ala Glu 
    1220                 1225                 1230             


Thr Leu  His Tyr Lys Lys Asp  His Phe Gly Asn Glu  Leu Leu Asp 
    1235                 1240                 1245             


Glu Asn  Gly Lys Lys Ile Pro  Val Asp Phe Val Ser  Thr Gly Asn 
    1250                 1255                 1260             


Asn His  His Val Ala Ile Tyr  Glu Asp Ala Asp Gly  Asn Leu Gln 
    1265                 1270                 1275             


Glu Arg  Val Val Ser Phe Tyr  Glu Ala Val Glu Arg  Val Asn Gln 
    1280                 1285                 1290             


Gly Leu  Ser Ile Ile Asp Lys  Glu Tyr Asn Gln Asp  Leu Gly Trp 
    1295                 1300                 1305             


Lys Phe  Leu Phe Thr Met Lys  Gln Asn Glu Met Phe  Leu Phe Pro 
    1310                 1315                 1320             


Ser Glu  Asp Phe Asn Pro Lys  Glu Thr Asp Leu Phe  Asp Glu Lys 
    1325                 1330                 1335             


Asn Leu  Asn Leu Ile Ser Lys  Asn Met Phe Arg Val  Gln Lys Phe 
    1340                 1345                 1350             


Gly Ser  Leu Leu Ser Gly Phe  Trp Phe Arg His His  Leu Glu Thr 
    1355                 1360                 1365             


Thr Val  Asp Leu Asn Ser Ser  Leu Lys Asp Thr Ser  Tyr Lys Val 
    1370                 1375                 1380             


Ile Gln  Ser Cys Asn Asn Leu  Lys Leu Ile Val Lys  Val Arg Leu 
    1385                 1390                 1395             


Asn His  Leu Gly Lys Ile Val  Gln Ile Gly Glu Tyr  
    1400                 1405                 1410 


<210>  1197
<211>  1411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1197

Met Val Lys Lys Glu Tyr Phe Ile Gly Leu Asp Met Gly Thr Ser Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Ala Asp Thr Asn Tyr Asn Leu Leu Arg Ala Lys 
            20                  25                  30          


Gly Lys Asp Met Trp Gly Val Arg Leu Phe Pro Glu Ala Ser Thr Ala 
        35                  40                  45              


Ala Asp Arg Arg Ser His Arg Thr Ala Arg Arg Asn Asn Lys Arg Lys 
    50                  55                  60                  


Ala Ala Arg Glu Gly Phe Leu Arg Glu Ile Phe Glu Asp Glu Ile Asn 
65                  70                  75                  80  


Lys Ile Asp Pro Gly Phe Phe Glu Arg Leu Lys Glu Ser Lys Tyr Tyr 
                85                  90                  95      


Leu Glu Asp Lys Leu Thr Asp Thr Pro Phe Val Leu Phe Ala Asp Lys 
            100                 105                 110         


Gly Tyr Thr Asp Lys Glu Tyr Tyr Asp Glu Tyr Pro Thr Val Phe His 
        115                 120                 125             


Leu Ile Lys Glu Leu Ile Ser Glu Lys Gly Leu Glu Pro His Asp Ile 
    130                 135                 140                 


Arg Leu Val Tyr Leu Ala Cys Leu Asn Ile Tyr Lys His Arg Gly His 
145                 150                 155                 160 


Phe Leu Asn Asp Thr Leu Ser Gly Asp Gly Ile Asp Asn Ile Asn Ser 
                165                 170                 175     


Leu Cys Asn Asp Phe Thr Ser Tyr Leu Ala Asp Tyr Asn Gln Asn Ser 
            180                 185                 190         


Glu Asp Asp Gly Thr Glu Lys Met Asp Leu Asp Ser Thr Val Leu Ala 
        195                 200                 205             


Gly Val Phe Glu Glu Thr Leu Cys Asn Lys His Leu Ser Asn Ser Ala 
    210                 215                 220                 


Lys Lys Arg Asp Leu Ile Asp Lys Leu Gly Ile Thr Asn Lys Thr Lys 
225                 230                 235                 240 


Tyr Leu Thr Glu Met Met Ala Leu Ile Ser Gly Leu Ala Ser Ser Val 
                245                 250                 255     


Ser Asn Met Phe Ala Lys Asp Thr Tyr Asp Glu Glu Gln Lys Lys Phe 
            260                 265                 270         


Lys Leu Ser Phe Arg Ser Ser Asn Leu Asp Glu Asp Met Val Lys Val 
        275                 280                 285             


Gln Ala Met Leu Thr Pro Glu Glu Tyr Glu Met Ile Cys Ile Met Lys 
    290                 295                 300                 


Lys Ile His Asp Trp Ser Val Val Ser Val Ile Met Gly Ser His Lys 
305                 310                 315                 320 


Tyr Ile Ser Asp Ala Arg Val Glu Leu Tyr Glu Lys His Lys Gln Asp 
                325                 330                 335     


Leu Ala Lys Leu Lys Ala Ile Tyr Lys Glu Tyr Leu Pro Asp Lys Tyr 
            340                 345                 350         


Asp Ala Met Phe Arg Glu Met Thr Asp Asn Ser Tyr Ser Ala Tyr Val 
        355                 360                 365             


Gly Lys Val Ser Ser Asn Lys Lys Ser Gln Thr Gly Asn His Lys Val 
    370                 375                 380                 


Arg Met Gln Arg Arg Gly Ala Lys Cys Lys Gln Glu Asp Phe Tyr Asp 
385                 390                 395                 400 


Arg Ile Lys Lys Glu Ile Asn Ser Val Pro Gln Glu Glu Lys Ile Asp 
                405                 410                 415     


Ile Leu Gln Asp Met Glu Thr Asn Ser Phe Leu Pro Lys Gln Leu Thr 
            420                 425                 430         


Asn Glu Asn Gly Val Ile Pro Tyr Gln Leu His Leu Ala Glu Leu Lys 
        435                 440                 445             


Lys Ile Leu Asp Asn Ala Ser Thr Tyr Leu Pro Phe Leu Asn Ser Ala 
    450                 455                 460                 


Asp Glu Thr Gly Ile Ser Asn Lys Asp Lys Ile Ile Gln Leu Phe Glu 
465                 470                 475                 480 


Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Phe Lys Ser Glu Lys Asn 
                485                 490                 495     


Lys Thr Asn Ala Trp Val Val Arg Lys Glu Ser Gly Lys Val Tyr Pro 
            500                 505                 510         


Trp Asn Phe Asn Glu Lys Ile Asp Glu Lys Gln Ser Ala Glu Ile Phe 
        515                 520                 525             


Ile Glu Arg Met Val Arg His Cys Thr Tyr Leu Asn Asn Glu Ser Ala 
    530                 535                 540                 


Leu Pro Lys Asn Ser Leu Lys Tyr Glu Arg Phe Arg Val Leu Asn Glu 
545                 550                 555                 560 


Leu Asn Asn Leu Arg Ile Asn Gly Glu Arg Ile Ser Pro Glu Leu Lys 
                565                 570                 575     


Gln Arg Ile Tyr Asn Glu Leu Phe Lys Val Lys Lys Lys Arg Ile Thr 
            580                 585                 590         


Lys Ser Met Leu Cys Glu Tyr Leu Tyr Lys Asn Gly Ile Ala Asp Lys 
        595                 600                 605             


Asp Ser Glu Leu Ser Gly Phe Asp Gly Thr Phe Ala Asn Ala Leu Leu 
    610                 615                 620                 


Ser Tyr Gly Lys Phe Arg Glu Leu Phe Asn Val Asp Thr Leu Thr Asp 
625                 630                 635                 640 


Lys Gln Glu Ala Ala Ala Glu Asp Ile Ile Lys Trp Ser Thr Val Tyr 
                645                 650                 655     


Gly Asp Ser Lys Lys Phe Leu Gln Glu Lys Ile Glu Glu Asn Tyr Gly 
            660                 665                 670         


Pro Glu Ser Glu Asn Pro Leu Leu Asp Asn Lys Gln Ile Lys Arg Val 
        675                 680                 685             


Met Gly Tyr Lys Phe Thr Asp Trp Gly Asn Leu Ser Arg Glu Phe Leu 
    690                 695                 700                 


Asn Leu Glu Gly Ala Asp Lys Asp Asn Asp Thr Thr Thr Gly Glu Val 
705                 710                 715                 720 


Met Pro Leu Ile Thr Arg Met Trp Glu Glu Asn Tyr Asn Leu Met Glu 
                725                 730                 735     


Leu Met Ser Asn Lys Phe Ser Tyr Arg Asp Ala Leu Ala Glu Lys Thr 
            740                 745                 750         


Glu Glu Ile Glu Lys Thr Leu Thr Asp Ile Glu Tyr Glu Asp Leu Glu 
        755                 760                 765             


Asp Leu Tyr Ile Ser Ala Pro Val Lys Arg Met Thr Trp Gln Thr Leu 
    770                 775                 780                 


Leu Ile Leu Lys Glu Ile Tyr Gln Ile Met Gly Tyr Ala Pro Lys Lys 
785                 790                 795                 800 


Ile Phe Val Glu Met Ala Arg Glu Glu Glu Glu Lys Val Asn Gly Lys 
                805                 810                 815     


Gly Ala Arg Lys Ser Ser Arg Lys Lys Arg Leu Glu Asp Leu Tyr Arg 
            820                 825                 830         


Asn Cys Lys Asp Ala Ser Lys Glu Leu Ser Thr Gly Ile Lys Asn Ala 
        835                 840                 845             


Asp Glu Ala Ile Leu Arg Arg Lys Lys Leu Tyr Leu Tyr Tyr Leu Gln 
    850                 855                 860                 


Lys Gly Lys Cys Met Tyr Ser Gly Lys Thr Ile Asp Phe Lys Ser Leu 
865                 870                 875                 880 


Phe Thr Asn Asn Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Val Val 
                885                 890                 895     


Lys Asp Asp Ser Leu Glu Asn Asn Met Val Leu Val Glu Arg Asn Tyr 
            900                 905                 910         


Asn Asn Lys Lys Ser Asp Thr Phe Pro Ile Phe Ser Asp Trp Gln Asn 
        915                 920                 925             


Lys Met Lys Pro Phe Trp Thr Met Leu Lys Glu Gln Gly Phe Ile Asn 
    930                 935                 940                 


Glu Glu Lys Tyr Lys Arg Leu Thr Arg Thr Asp Glu Leu Thr Asp Asp 
945                 950                 955                 960 


Glu Leu Ala Asn Phe Val Asn Arg Gln Leu Val Glu Thr Arg Gln Ala 
                965                 970                 975     


Thr Lys Val Ile Ala Glu Leu Leu Lys Asn Ser Phe Asp Asp Ile Asp 
            980                 985                 990         


Ser Lys Ile Val Tyr Ala Lys Ala  Gly Asn Val Ser Glu  Phe Arg His 
        995                 1000                 1005             


Lys Phe  Ser Met Lys Tyr Asp  Lys Glu Thr Glu Lys  Ser Ser Val 
    1010                 1015                 1020             


Ile His  Pro Glu Leu Val Lys  Cys Arg Ile Ile Asn  Asp Phe His 
    1025                 1030                 1035             


His Ala  Asn Asp Ser Tyr Leu  Asn Ile Val Val Gly  Asn Ile Tyr 
    1040                 1045                 1050             


Asp Val  Lys Phe Thr Gln Asn  Pro Tyr Asn Tyr Ile  Lys Glu Tyr 
    1055                 1060                 1065             


Lys Lys  Gln Lys Asn Asn Ser  Ala Leu Ser Glu Gln  Glu Lys Glu 
    1070                 1075                 1080             


Lys Tyr  His Met Asp Lys Ile  Phe Asn Phe Asp Val  Lys Arg Asn 
    1085                 1090                 1095             


Gly Glu  Val Ala Trp Ile Ser  Arg Gly Asp Asn Lys  Thr Leu Glu 
    1100                 1105                 1110             


Thr Val  Leu Lys Val Met Arg  Lys Asn Thr Pro Ile  Val Thr Arg 
    1115                 1120                 1125             


Met Asn  Phe Glu Glu His Gly  Gln Leu Ala Asp Leu  Thr Ile Tyr 
    1130                 1135                 1140             


Ser Ala  Ser Asp Ala Leu Ser  Lys Lys Gly Val Gly  Tyr Ile Lys 
    1145                 1150                 1155             


Val Lys  Ala Ser Asp Ala Arg  Leu Asp Val Leu Lys  Tyr Gly Gly 
    1160                 1165                 1170             


Tyr Lys  Lys Tyr Lys Gly Ala  Tyr Phe Phe Leu Val  Glu His Thr 
    1175                 1180                 1185             


Glu Lys  Asn Lys Arg Val Arg  Thr Ile Glu Asn Met  Pro Leu Tyr 
    1190                 1195                 1200             


Leu Lys  Asn Glu Leu Asn Thr  Lys Glu Lys Ile Glu  Leu Trp Cys 
    1205                 1210                 1215             


Ala Asp  Lys Glu Asn Gly Leu  Gly Leu Val Asp Pro  Asp Val Arg 
    1220                 1225                 1230             


Leu Gly  Lys Ile Lys Leu Tyr  Ser Lys Ile Arg Ile  Asp Gly Phe 
    1235                 1240                 1245             


Asp Leu  Tyr Leu Thr Gly Arg  Thr Gly Asn Asn Leu  Leu Thr Ser 
    1250                 1255                 1260             


Asn Ala  Val Gln Leu Lys Ile  Gly Ser Asn Lys Pro  Ser Asp Asn 
    1265                 1270                 1275             


Asp Lys  Leu Phe Trp Arg Tyr  Tyr Ile Asn Lys Leu  Ser Lys Tyr 
    1280                 1285                 1290             


Thr Glu  Asn Ser Glu Phe Val  Ser Ile Gln Asn Asn  Ile Lys Leu 
    1295                 1300                 1305             


Tyr Asp  Leu Phe Val Glu Ile  Asn Asn Ala Gly Val  Tyr Ser Lys 
    1310                 1315                 1320             


Arg Gln  Asn Pro Ile Gly Asp  Lys Leu Leu Glu Gly  Arg Ser Lys 
    1325                 1330                 1335             


Phe Ile  Asn Leu Thr Leu Ser  Lys Gln Val Glu Thr  Leu Leu Gly 
    1340                 1345                 1350             


Ile Ile  Arg Ile Tyr Ser Leu  Glu Asn Gln Gly Ala  Asp Leu Ser 
    1355                 1360                 1365             


Asn Ile  Gly Gly Gln Glu Lys  Asn Gly Thr Met Lys  Pro His Lys 
    1370                 1375                 1380             


Arg Ile  Ser Asp Lys Lys Glu  Val Leu Leu Ile Asn  Gln Ser Val 
    1385                 1390                 1395             


Thr Gly  Leu Tyr Glu Ser Lys  Ile Asp Leu Leu Lys  Ile 
    1400                 1405                 1410     


<210>  1198
<211>  1411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1198

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Lys Gln Asn Ala Glu Glu Arg Gln Gly Ser Ile Leu Gly 
            20                  25                  30          


Met Gly Thr Arg Val Leu Pro Met Ser Gln Asp Val Leu Gly Asp Phe 
        35                  40                  45              


Gly Lys Gly Asn Ser Val Ser Gln Thr Ala Ala Arg Thr Gly Tyr Arg 
    50                  55                  60                  


Gly Ile Arg Arg Leu Arg Glu Arg Phe Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Asn Ile Leu Gly Phe Leu Pro Tyr Tyr Tyr Ala Ala 
                85                  90                  95      


Gln Ile Asp Phe Asp Lys Arg Phe Gly Lys Phe Lys Ala Glu Thr Glu 
            100                 105                 110         


Pro Lys Leu Pro Tyr Asn Asn Glu Gly Phe Ile Phe Lys Asn Ser Phe 
        115                 120                 125             


Glu Glu Met Leu Ser Asp Phe Lys Ile Ser Gln Pro Glu Leu Leu Ser 
    130                 135                 140                 


Gly Asn Lys Lys Val Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys 
145                 150                 155                 160 


Lys Ala Val Ser Gln Lys Ile Glu Lys Glu Glu Leu Ala Trp Ile Leu 
                165                 170                 175     


Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Asp 
            180                 185                 190         


Phe Asp Glu Glu Lys Asp Lys Thr Phe Val Thr Leu Asn Val Asp Lys 
        195                 200                 205             


Val Ile Asp Ser Gly Glu Lys Val Lys Gly Asn Ile Leu Phe Asp Ile 
    210                 215                 220                 


Tyr Phe Thr Asn Gly Trp Lys Tyr Glu Lys Gln Thr Thr Lys Pro Glu 
225                 230                 235                 240 


Asp Trp Leu Gly Arg Ser Lys Glu Phe Ile Ile Thr Glu Ser Leu Leu 
                245                 250                 255     


Lys Asn Gly Glu Val Arg Arg Thr Phe Lys Ala Val Asp Ser Glu Lys 
            260                 265                 270         


Asp Trp Ile Ala Ile Lys Ala Lys Thr Glu Gln Glu Ile Glu Thr Ala 
        275                 280                 285             


His Lys Thr Val Gly Ser Tyr Ile Tyr Asp Ala Ile Leu Gln Asn Pro 
    290                 295                 300                 


Lys Gln Lys Ile Lys Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Phe 
305                 310                 315                 320 


Tyr Lys Glu Glu Leu Arg Lys Ile Leu Glu Lys Gln Lys Asp Phe His 
                325                 330                 335     


Leu Glu Leu Gln Asn Glu Asp Leu Tyr Ser Asp Cys Val Arg Glu Leu 
            340                 345                 350         


Tyr Arg Asn Asn Asp Thr His Gln Leu Ser Leu Ala Lys Lys Ser Phe 
        355                 360                 365             


Ile His Leu Phe Leu Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys 
    370                 375                 380                 


Ser Gln Lys Ser Ser Val Ser Asn Cys Thr Leu Glu Cys Arg Lys Tyr 
385                 390                 395                 400 


Arg Asp Lys Asn Gly Ala Glu Gly Ile Gln Phe Leu Lys Val Ile Pro 
                405                 410                 415     


Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Ile Trp Gln Trp Met Ser 
            420                 425                 430         


Asn Leu Lys Ile Tyr Arg Arg Asp Asp Asp Val Asn Ile Thr Ser Glu 
        435                 440                 445             


Cys Leu Lys Thr Ala Glu Asp Tyr Glu Asp Leu Phe Val Phe Leu Asn 
    450                 455                 460                 


Ile Arg Lys Glu Val Asp Gln Lys Ala Cys Leu Arg His Phe Lys Leu 
465                 470                 475                 480 


Ser Glu Lys Thr His Arg Trp Asn Tyr Val Glu Asp Lys Lys Tyr Pro 
                485                 490                 495     


Cys Asn Glu Thr Gly Thr Met Ile Ser Glu Arg Leu Lys Lys Val Gly 
            500                 505                 510         


Asp Ser Pro Asp Asn Ile Phe Thr Arg Glu Met Glu Gln Gln Ile Trp 
        515                 520                 525             


His Ile Ile Tyr Ser Val Asn Asp Lys Thr Glu Tyr Glu Lys Ala Leu 
    530                 535                 540                 


Lys Ser Phe Ala Lys Lys Asn Glu Leu Asp Asn Asp Ser Phe Phe Glu 
545                 550                 555                 560 


Ala Phe Arg Lys Phe Pro Pro Phe Asp Ser Glu Tyr Gly Ser Phe Ser 
                565                 570                 575     


Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Ile Gly Lys His 
            580                 585                 590         


Trp Asn Trp Glu Ala Val Asp Glu Lys Ile Lys Asn Arg Ile Gln Lys 
        595                 600                 605             


Ile Ile Ile Gly Glu Tyr Asp Glu Asn Ile Lys Asp Asn Ile Arg Glu 
    610                 615                 620                 


Lys Thr Leu Ser Leu Gln Lys Glu Ser Asp Phe Gln Gly Leu Pro Leu 
625                 630                 635                 640 


Trp Leu Ala Gln Tyr Val Val Tyr Gly Arg His Ser Glu Ala Ser Val 
                645                 650                 655     


Ala Gly Lys Trp Asn Ser Val Asp Asp Ile Glu Glu Tyr Leu Lys Glu 
            660                 665                 670         


Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Met 
        675                 680                 685             


Glu Thr Val Arg Val Val Lys Asp Ile Trp Ile Lys Tyr Gly Asn Gly 
    690                 695                 700                 


Ala Ala Asp Phe Phe Asp Glu Ile His Ile Glu Leu Gly Arg Glu Met 
705                 710                 715                 720 


Lys Leu Pro Ala Lys Glu Arg Asn Lys Met Thr Gln Lys Ile Ser Glu 
                725                 730                 735     


Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala Leu Leu Ala Glu Met Met 
            740                 745                 750         


Asn Asp Ser Glu Val Gln Asn Val Arg Pro Tyr Ser Pro Met Gln Gln 
        755                 760                 765             


Glu Ile Leu Lys Ile Tyr Glu Asp Gly Ala Leu Asn Ser Glu Ile Ser 
    770                 775                 780                 


Val Asp Asp Asp Ile Leu Lys Ile Ser Lys Thr Ala Gln Pro Ser Pro 
785                 790                 795                 800 


Ser Asp Leu Lys Lys Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser 
                805                 810                 815     


Pro Tyr Thr Gly Asp Ile Ile Pro Leu Asn Lys Leu Phe Thr Thr Glu 
            820                 825                 830         


Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser 
        835                 840                 845             


Phe Ser Asn Lys Val Ile Cys Glu Ser Leu Val Asn Arg Leu Lys Asp 
    850                 855                 860                 


Asn Tyr Ile Gly Leu Gly Phe Ile Lys Gln Phe His Gly Tyr Lys Ile 
865                 870                 875                 880 


Ser Asp Lys Thr Lys Val Phe Glu Val Glu Glu Tyr Glu Asp Phe Val 
                885                 890                 895     


Lys Lys Asn Tyr Ala Asn Asn Arg Ser Lys Arg Ser Lys Leu Leu Leu 
            900                 905                 910         


Glu Glu Ile Pro Glu Glu Met Ile Glu Arg Gln Leu Asn Asp Thr Arg 
        915                 920                 925             


Tyr Ile Ser Lys Tyr Ile Ser Gly Ile Leu Ser Asn Ile Val Arg Lys 
    930                 935                 940                 


Lys Glu Asn Asp Glu Gly Val Asn Ser Lys Asp Ile Ile Pro Gly Asn 
945                 950                 955                 960 


Gly Lys Ile Thr Ser Gln Leu Lys Gln Asp Trp Gly Leu Asn Asp Val 
                965                 970                 975     


Trp Asn Asp Leu Ile Leu Pro Arg Phe Glu Arg Met Asn Glu Leu Thr 
            980                 985                 990         


Lys Ser Ser Tyr Phe Thr Val Trp  Asn Glu Lys Tyr Gln  Lys Phe Leu 
        995                 1000                 1005             


Pro Thr  Val Pro Val Glu Phe  Ser Lys Gly Phe Ser  Lys Lys Arg 
    1010                 1015                 1020             


Ile Asp  His Arg His His Ala  Leu Asp Ala Leu Val  Ile Ala Cys 
    1025                 1030                 1035             


Ala Thr  Lys Asp His Val Asn  Leu Leu Asn Asn Lys  Ser Ala Lys 
    1040                 1045                 1050             


Ser Glu  Met Lys Arg Tyr Asp  Leu Lys Arg Lys Leu  Met Lys Phe 
    1055                 1060                 1065             


Glu Lys  Lys Thr Tyr Gln His  Ser Gln Thr Gly Glu  Arg Ile Val 
    1070                 1075                 1080             


Lys Asp  Val Pro Val Glu Phe  Leu Lys Pro Trp Asn  Asp Phe Thr 
    1085                 1090                 1095             


Val Glu  Ala Lys Glu Asn Leu  Glu Lys Ile Ile Val  Ser Phe Lys 
    1100                 1105                 1110             


Gln Asn  Leu Arg Val Ile Asn  Lys Ala Thr Asn His  Tyr Glu Lys 
    1115                 1120                 1125             


Trp Ile  Glu Lys Asp Asn Val  Lys Val Lys Gly Leu  Val Glu Gln 
    1130                 1135                 1140             


Gln Gly  Leu Asn Trp Ala Ile  Arg Lys Pro Leu His  Lys Asp Thr 
    1145                 1150                 1155             


Val Ser  Gly Lys Val Asp Leu  Pro Gly Ile Lys Val  Pro Lys Gly 
    1160                 1165                 1170             


Lys Ile  Leu Thr Ala Thr Arg  Lys Ser Ile Asp Thr  Ser Phe Asp 
    1175                 1180                 1185             


Leu Lys  Thr Ile Asn Ser Ile  Thr Asp Thr Gly Val  Gln Lys Ile 
    1190                 1195                 1200             


Leu Lys  Asn Tyr Leu Glu Tyr  Lys Gly Asn Ser Ser  Glu Glu Ala 
    1205                 1210                 1215             


Phe Ser  Ser Glu Gly Ile Glu  Asp Met Asn Asn Asn  Ile Glu Lys 
    1220                 1225                 1230             


Phe Asn  Glu Gly Lys Leu His  Gln Pro Ile Thr Lys  Val Arg Ile 
    1235                 1240                 1245             


Phe Glu  Leu Gly Ser Lys Phe  Pro Leu Gly Gln Ile  Gly Asn Lys 
    1250                 1255                 1260             


Lys His  Lys Tyr Val Glu Thr  Ala Lys Gly Thr Asn  Leu Phe Phe 
    1265                 1270                 1275             


Ala Ile  Tyr Glu Asp Asp Lys  Gly Lys Arg Thr Tyr  Glu Thr Ile 
    1280                 1285                 1290             


Pro Leu  Asn Glu Val Ile Glu  Arg Gln Lys Gln Gly  Leu Ala Pro 
    1295                 1300                 1305             


Val Thr  Leu Lys Ser Lys Asn  Asp Phe Tyr Leu Cys  Pro Asn Asp 
    1310                 1315                 1320             


Leu Val  Tyr Val Ala Ala Glu  Gly Glu Ser Val Asn  Lys Ile Asp 
    1325                 1330                 1335             


Phe Lys  Tyr Ala Ser Gln Glu  Phe Ile His Asn Ile  Tyr Lys Val 
    1340                 1345                 1350             


Val Ser  Phe Ser Gly Ser Gln  Ile Phe Phe Val Lys  Gln Asn Ile 
    1355                 1360                 1365             


Ala Val  Ser Ile Val Asn Lys  Ala Glu Phe Ser Thr  Leu Asn Lys 
    1370                 1375                 1380             


Met Glu  Arg Ala Val Asp Gly  Lys Met Ile Lys Asp  Cys Cys Ile 
    1385                 1390                 1395             


Lys Leu  Lys Ile Asp Arg Ile  Gly Asn Ile Thr Lys  Ser 
    1400                 1405                 1410     


<210>  1199
<211>  1412
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1199

Met Met Thr Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Trp Ala Leu Ile Lys Gln Asn Ala Glu Glu Arg Lys Gly Ser Ile 
            20                  25                  30          


Leu Gly Met Gly Thr Arg Val Leu Pro Met Ser Gln Asp Ala Leu Gly 
        35                  40                  45              


Asp Phe Gly Lys Gly Asn Ser Val Ser Gln Thr Ala Ala Arg Thr Gly 
    50                  55                  60                  


Tyr Arg Gly Val Arg Arg Leu Arg Glu Arg Phe Leu Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Arg Val Leu Asn Ile Leu Gly Phe Leu Pro Asp Tyr Tyr 
                85                  90                  95      


Ala Ala Gln Ile Asp Phe Asp Lys Arg Phe Gly Lys Phe Lys Ala Glu 
            100                 105                 110         


Thr Glu Pro Lys Leu Pro Tyr Asn Asn Glu Gly Phe Ile Phe Lys Asn 
        115                 120                 125             


Ser Phe Glu Glu Met Leu Ser Asp Phe Lys Ile Ser Gln Pro Glu Leu 
    130                 135                 140                 


Leu Ser Glu Asn Lys Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu 
145                 150                 155                 160 


Arg Lys Lys Ala Val Ser Gln Arg Ile Glu Lys Glu Glu Leu Val Trp 
                165                 170                 175     


Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly 
            180                 185                 190         


Glu Asp Phe Asp Glu Glu Lys Asp Lys Thr Phe Val Thr Leu Asn Val 
        195                 200                 205             


Asp Lys Val Ile Asp Ser Gly Glu Lys Val Lys Gly Ile Val Leu Phe 
    210                 215                 220                 


Asp Val Tyr Phe Thr Asn Gly Trp Lys Tyr Glu Lys Gln Ile Thr Lys 
225                 230                 235                 240 


Pro Glu Asp Trp Leu Gly Arg Ser Lys Glu Phe Ile Ile Thr Glu Ser 
                245                 250                 255     


Leu Leu Lys Asn Gly Glu Val Lys Arg Thr Phe Lys Ala Val Asp Ser 
            260                 265                 270         


Glu Lys Asp Trp Ile Ala Ile Lys Ala Lys Thr Glu Gln Glu Ile Glu 
        275                 280                 285             


Ile Ala His Lys Thr Val Gly Ser Tyr Ile Tyr Asp Ala Ile Leu Gln 
    290                 295                 300                 


Asn Pro Lys Gln Lys Ile Lys Gly Lys Leu Ile Arg Thr Ile Glu Arg 
305                 310                 315                 320 


Lys Phe Tyr Lys Glu Glu Leu Arg Lys Ile Leu Glu Lys Gln Lys Glu 
                325                 330                 335     


Phe His Pro Glu Leu Gln Asn Glu Asp Leu Tyr Ser Asp Cys Val Arg 
            340                 345                 350         


Glu Leu Tyr Arg Asn Asn Asp Ala His Gln Leu Ser Leu Ala Lys Lys 
        355                 360                 365             


Asp Phe Ile His Leu Phe Val Glu Asp Ile Ile Phe Tyr Gln Arg Pro 
    370                 375                 380                 


Leu Lys Ser Gln Lys Ser Ser Val Ser Asn Cys Thr Leu Glu Cys Arg 
385                 390                 395                 400 


Lys Tyr Lys Asp Lys Asn Gly Glu Glu Arg Ile Gln Phe Leu Lys Val 
                405                 410                 415     


Ile Pro Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Ile Trp Gln Trp 
            420                 425                 430         


Met Ser Asn Leu Lys Ile Tyr Arg Arg Asp Asp Asp Val Asn Ile Thr 
        435                 440                 445             


Ser Glu Tyr Leu Lys Thr Thr Glu Asp Tyr Gln Asp Leu Phe Val Phe 
    450                 455                 460                 


Leu Asn Thr Arg Lys Glu Val Asp Gln Lys Ala Cys Leu Lys His Phe 
465                 470                 475                 480 


Lys Leu Asn Glu Lys Thr His Arg Trp Asn Tyr Val Glu Asp Lys Arg 
                485                 490                 495     


Tyr Pro Cys Asn Glu Thr Gly Thr Met Ile Leu Glu Arg Phe Lys Lys 
            500                 505                 510         


Val Gly Asp Ser Ala Asp Asn Ile Phe Thr Arg Glu Met Glu Gln Gln 
        515                 520                 525             


Met Trp His Ile Ile Tyr Ser Val Asn Asp Lys Thr Glu Tyr Glu Lys 
    530                 535                 540                 


Ala Leu Lys Ser Phe Ala Lys Lys Asn Asp Leu Asp Asn Val Ser Phe 
545                 550                 555                 560 


Phe Glu Ala Phe Arg Lys Phe Pro Pro Phe Asp Ser Glu Tyr Gly Ser 
                565                 570                 575     


Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu Ile Arg Thr Gly 
            580                 585                 590         


Lys Tyr Trp Asn Trp Glu Ala Val Asp Glu Lys Thr Lys Lys Arg Ile 
        595                 600                 605             


Gln Lys Met Ile Thr Gly Glu Trp Asp Glu Asp Ile Lys Asp Asn Ile 
    610                 615                 620                 


Arg Glu Lys Thr Leu Ser Leu Gln Lys Glu Ser Asp Phe Gln Gly Leu 
625                 630                 635                 640 


Pro Leu Trp Leu Ala Gln Tyr Ala Val Tyr Gly Arg His Ser Glu Ala 
                645                 650                 655     


Ser Val Ala Gly Lys Trp Asn Ser Val Asn Asp Met Glu Glu Tyr Leu 
            660                 665                 670         


Lys Glu Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val 
        675                 680                 685             


Ile Thr Glu Thr Ile Arg Val Val Lys Asp Ile Trp Ile Lys Tyr Gly 
    690                 695                 700                 


Asn Gly Ala Ala Asp Phe Phe Asn Glu Ile His Ile Glu Leu Gly Arg 
705                 710                 715                 720 


Glu Met Lys Leu Pro Ala Lys Glu Arg Ser Lys Met Thr Gln Lys Ile 
                725                 730                 735     


Ser Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala Leu Leu Val Glu 
            740                 745                 750         


Met Met Asn Asp Ser Glu Val His Asn Val Arg Pro Tyr Ser Pro Met 
        755                 760                 765             


Gln Gln Glu Ile Leu Lys Ile Tyr Glu Asp Gly Ala Leu Asn Ser Glu 
    770                 775                 780                 


Ile Ser Val Asp Asp Asp Ile Leu Lys Ile Ser Lys Thr Ala Gln Pro 
785                 790                 795                 800 


Ser Pro Ser Asp Leu Lys Lys Tyr Lys Leu Trp Leu Glu Gln Lys Tyr 
                805                 810                 815     


Arg Ser Pro Tyr Thr Gly Asp Ile Ile Pro Leu Asn Lys Leu Phe Thr 
            820                 825                 830         


Thr Glu Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Phe Asp 
        835                 840                 845             


Asp Ser Phe Ser Asn Lys Val Ile Cys Glu Ser Ser Val Asn Arg Leu 
    850                 855                 860                 


Lys Asp Asn Tyr Ile Gly Leu Gly Phe Ile Lys Gln Phe His Gly Tyr 
865                 870                 875                 880 


Lys Ile Ser Asp Lys Thr Lys Val Phe Glu Val Glu Glu Tyr Glu Asp 
                885                 890                 895     


Phe Val Lys Lys Asn Tyr Ala Ser Asn Trp Ser Lys Arg Ser Lys Leu 
            900                 905                 910         


Leu Leu Glu Glu Ile Pro Glu Glu Met Ile Glu Arg Gln Leu Asn Asp 
        915                 920                 925             


Thr Arg Tyr Ile Ser Lys Tyr Ile Ser Gly Ile Leu Ser Asn Ile Val 
    930                 935                 940                 


Arg Lys Lys Glu Asn Asp Asp Gly Val Asn Ser Lys Asp Ile Ile Pro 
945                 950                 955                 960 


Gly Asn Gly Lys Ile Thr Ser Gln Leu Lys Gln Asp Trp Gly Leu Asn 
                965                 970                 975     


Asp Val Trp Asn Asp Leu Val Leu Pro Arg Phe Glu Arg Met Asn Glu 
            980                 985                 990         


Leu Thr Lys Ser Asn Asp Phe Thr  Val Trp Asn Glu Lys  Tyr Gln Lys 
        995                 1000                 1005             


Phe Leu  Pro Thr Val Pro Val  Glu Phe Ser Lys Gly  Phe Ser Lys 
    1010                 1015                 1020             


Lys Arg  Ile Asp His Arg His  His Ala Leu Asp Ala  Leu Val Ile 
    1025                 1030                 1035             


Ala Cys  Ala Thr Arg Asp His  Val Asn Leu Leu Asn  Asn Gln Ser 
    1040                 1045                 1050             


Ala Arg  Ser Glu Met Lys Arg  Tyr Asp Leu Lys Arg  Lys Leu Met 
    1055                 1060                 1065             


Lys Phe  Glu Lys Lys Ala Tyr  Gln His Ser Gln Thr  Gly Glu Arg 
    1070                 1075                 1080             


Ile Val  Lys Asp Val Pro Val  Glu Phe Leu Lys Pro  Trp Asp Asn 
    1085                 1090                 1095             


Phe Ile  Val Glu Ala Lys Glu  Asn Leu Asp Arg Ile  Ile Val Ser 
    1100                 1105                 1110             


Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys Ala Thr  Asn His Tyr 
    1115                 1120                 1125             


Glu Lys  Trp Ile Glu Lys Asn  Asn Val Lys Val Lys  Ala Leu Val 
    1130                 1135                 1140             


Glu Gln  Gln Gly Leu Asn Trp  Ala Ile Arg Lys Pro  Leu His Lys 
    1145                 1150                 1155             


Asp Thr  Val Ser Gly Lys Val  Asp Leu Pro Gly Thr  Lys Val Pro 
    1160                 1165                 1170             


Lys Gly  Lys Ile Leu Thr Ala  Thr Arg Lys Ser Ile  Asp Thr Ser 
    1175                 1180                 1185             


Phe Asp  Met Lys Thr Ile Asn  Ser Ile Thr Asp Thr  Gly Ile Gln 
    1190                 1195                 1200             


Lys Ile  Leu Lys Asn Tyr Leu  Glu Tyr Lys Gly Ser  Tyr Glu Asp 
    1205                 1210                 1215             


Ala Phe  Ser Pro Glu Gly Ile  Glu Asp Met Asn Lys  Asn Ile Glu 
    1220                 1225                 1230             


Lys Phe  Asn Asp Gly Lys Gln  His Gln Pro Ile Ile  Lys Val Arg 
    1235                 1240                 1245             


Ile Phe  Glu Leu Gly Ser Lys  Phe Pro Leu Gly Gln  Ile Gly Asn 
    1250                 1255                 1260             


Lys Lys  His Lys Tyr Val Glu  Thr Ala Lys Gly Thr  Asn Leu Phe 
    1265                 1270                 1275             


Phe Ala  Ile Tyr Glu Asp Asp  Lys Gly Lys Arg Thr  Tyr Glu Thr 
    1280                 1285                 1290             


Ile Pro  Leu Asn Glu Val Ile  Glu Arg Gln Lys Gln  Gly Leu Ala 
    1295                 1300                 1305             


Pro Val  Thr Phe Lys Ser Lys  Asn Asp Phe Tyr Leu  Ser Pro Asn 
    1310                 1315                 1320             


Asp Leu  Val Tyr Val Ala Ala  Glu Gly Glu Ser Val  Asp Asn Leu 
    1325                 1330                 1335             


Asp Phe  Gln Ser Asp Ser Gln  Glu Tyr Ile Asn Asn  Val Tyr Lys 
    1340                 1345                 1350             


Val Val  Ser Phe Ser Gly Ser  Gln Ile Phe Phe Val  Lys Gln Asn 
    1355                 1360                 1365             


Val Ala  Val Ser Ile Val Asn  Lys Ala Glu Phe Ser  Thr Leu Asn 
    1370                 1375                 1380             


Lys Met  Glu Arg Ala Ile Asp  Gly Arg Met Ile Lys  Glu Cys Cys 
    1385                 1390                 1395             


Val Lys  Leu Lys Ile Asp Arg  Ile Gly Asn Met Thr  Lys Ser 
    1400                 1405                 1410         


<210>  1200
<211>  1414
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1200

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Val Glu Glu Ala Glu Asn Glu Ser Glu Lys Ser Ser Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Gln Gln Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Gln Arg Arg Glu Asn 
65                  70                  75                  80  


Leu Ile Asp Ile Leu Met Lys Asn Gly Phe Thr Ser Asp Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ser Lys Ala Val Ala Glu Lys Ile Ser Leu Gln Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Thr Glu Asp Gly Glu Leu Ile Asp Gly Met Glu Val Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Asn Tyr Asn Leu Thr Pro Gly Gln Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asn Glu Gly Lys Lys His Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asp Arg Ile Phe Asp Ile Gln Met Thr Phe Tyr Thr 
        195                 200                 205             


Glu Tyr Leu Thr Leu Glu Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Ile Phe Lys Glu Lys Gly Phe 
225                 230                 235                 240 


Glu Leu Ile Gly Thr Lys Arg Gln Gly Ser Thr Ala Glu Gln Lys His 
                245                 250                 255     


Glu Asn Tyr Ser Trp Arg Ala Lys Gly Leu Ser Glu Lys Leu Glu Leu 
            260                 265                 270         


Glu Gln Leu Val Val Val Leu Gln Gln Ile Asn Ile Gln Leu Asn Ser 
        275                 280                 285             


Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Phe 
    290                 295                 300                 


Phe Asn Lys Gln Thr Val Gly Gln Tyr Gln Met Ala Val Leu Asp Lys 
305                 310                 315                 320 


Asn Pro Asn Ala Gly Leu Arg Asn Met Val Phe Tyr Arg Gln Asp Tyr 
                325                 330                 335     


Leu Asp Glu Phe Asn Ala Ile Trp Glu Lys Gln Ala Leu Phe His Pro 
            340                 345                 350         


Ile Leu Thr Asp Thr Leu Lys Asp Glu Ile Arg Asp Val Ile Ile Phe 
        355                 360                 365             


Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu 
    370                 375                 380                 


Leu Glu Ser Arg Lys Ile Glu Val Glu Ile Ala Gly Gln Asn Lys Thr 
385                 390                 395                 400 


Lys Met Val Gly Ser Arg Val Ile Pro Arg Ser Ser Pro Leu Phe Gln 
                405                 410                 415     


Glu Phe Lys Ile Trp Gln Ser Leu Asn Asn Ile Glu Val Phe Gly Lys 
            420                 425                 430         


Gly Lys Lys Ser Lys Lys Gln Lys Glu Gln Thr Leu Phe Asp Gln Thr 
        435                 440                 445             


Glu Asp Pro Leu Glu Phe Gly Gly Arg Arg Glu Leu Tyr Glu Glu Glu 
    450                 455                 460                 


Lys Glu Leu Leu Ala Leu Glu Leu Ser Ile Lys Lys Glu Leu Lys Lys 
465                 470                 475                 480 


Ala Asp Ile Leu Lys Leu Leu Phe Asp Asn Pro Lys Glu Leu Asp Leu 
                485                 490                 495     


Asn Phe Asn Ala Val Gln Gly Asn Leu Thr Gln Ala Thr Leu Phe Asn 
            500                 505                 510         


Val Tyr Lys Lys Ile Ile Glu Leu Ser Gly His Glu Ile Asp Val Glu 
        515                 520                 525             


Lys Asp Ser Ala Ser Lys Ile Leu Lys Ser Ile Glu Glu Ile Phe Lys 
    530                 535                 540                 


Ala Leu Asn Tyr Asn Thr Glu Ile Leu His Phe Asp Ser Glu Lys Ser 
545                 550                 555                 560 


Gln Asp Gln Gln Ala Met Tyr Met Leu Trp His Leu Leu Tyr Ser Phe 
                565                 570                 575     


Glu Gly Asp Asn Ser Asn Thr Gly Asn Glu Asn Leu Ile Asn Lys Ile 
            580                 585                 590         


Ser Lys Leu Tyr Gly Phe Glu Lys Glu Tyr Ala Lys Ile Leu Ala Asn 
        595                 600                 605             


Val Ser Phe Gln Asp Asp Tyr Gly Ser Leu Ser Ala Lys Ala Ile Ser 
    610                 615                 620                 


Lys Ile Leu Pro Tyr Leu Lys Glu Gly His Lys Ser Asp Ile Ala Cys 
625                 630                 635                 640 


Asp Leu Ala Gly Tyr Lys His Ser Ala Ser Ser Leu Thr Arg Glu Glu 
                645                 650                 655     


Leu Glu Asn Lys Thr Tyr Lys Asp Arg Leu Glu Ile Leu Pro Lys Asn 
            660                 665                 670         


Thr Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln Met Ile Asn 
        675                 680                 685             


Val Ile Asn Leu Ile Ile Asp Ser Arg Glu Leu Lys Lys Ser Ala Lys 
    690                 695                 700                 


Glu Arg Glu Glu Leu Thr Lys Ser Ile Ser Glu Thr Thr Lys Leu His 
705                 710                 715                 720 


Gln Gln Tyr Lys Asp Ile Leu Ala Lys Ala Pro Phe Asn Leu Thr Asn 
                725                 730                 735     


Thr Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Glu Glu Leu Lys 
            740                 745                 750         


Asp Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Lys Tyr Ile Ser Pro Ala 
        755                 760                 765             


Ala Leu Phe Ser Gly Asp Phe Asp Ile Glu His Ile Ile Pro Gln Ser 
    770                 775                 780                 


Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu Leu Arg Ser 
785                 790                 795                 800 


Ile Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe Ile Lys Gly 
                805                 810                 815     


Lys Tyr Gly Glu Thr Gly Glu Asp Asn Cys Leu Asp Lys Tyr Leu Asn 
            820                 825                 830         


Arg Ile Glu Thr Leu Tyr Lys Asn Gly Ile Leu Lys Lys Ser Lys Tyr 
        835                 840                 845             


Asn Lys Leu Lys Met Gln Asp Lys Asp Ile Pro Asp Gly Phe Ile Glu 
    850                 855                 860                 


Arg Asp Ile Arg Asp Thr Gln Tyr Ile Ala Lys Tyr Ala Lys Thr Leu 
865                 870                 875                 880 


Leu Gly Asp Leu Val Lys Phe Val Val Ser Thr Ser Gly Ser Val Thr 
                885                 890                 895     


Glu Arg Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu 
            900                 905                 910         


Asn Trp Glu Lys Tyr Met Gln Leu Gly Leu Thr Glu Glu Phe Thr Asn 
        915                 920                 925             


Glu Glu Gly His Arg Ile Arg Lys Ile Lys Asp Trp Thr Lys Arg Asn 
    930                 935                 940                 


Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys 
945                 950                 955                 960 


Arg Gln Phe Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Arg Ile Ser 
                965                 970                 975     


Asp Glu Gln Gly Ile Ser Asn Ser Glu Lys Asp Glu Arg Asn Asn Val 
            980                 985                 990         


Ser Ile Thr Thr Asn Asp Met Lys  Leu Asn Thr Arg Asp  Val Leu Gly 
        995                 1000                 1005             


Ile Glu  Lys Lys Glu Leu Tyr  Arg Asp Asn Arg Gly  Lys Leu Arg 
    1010                 1015                 1020             


Phe Lys  Ala Pro Met Pro Leu  Leu Glu Phe Arg Glu  Glu Ala Lys 
    1025                 1030                 1035             


Arg His  Leu Glu Asn Thr Leu  Ile Ser Ile Lys Ala  Lys Asn Lys 
    1040                 1045                 1050             


Val Thr  Thr Ile Asn Ile Asn  Thr Leu Lys Lys Ala  Gly Gly Val 
    1055                 1060                 1065             


Tyr Lys  Lys Thr Gln Leu Thr  Pro Arg Gly Gln Leu  His Asn Glu 
    1070                 1075                 1080             


Thr Ile  Tyr Gly Ser Gln Lys  Gln Tyr Ile Val Lys  Glu Glu Thr 
    1085                 1090                 1095             


Ile Asn  Ala Lys Phe Asp Val  Glu Lys Ile Ala Thr  Val Thr Asn 
    1100                 1105                 1110             


Pro Val  Tyr Arg Glu Ala Leu  Leu Lys Arg Leu Glu  Gln Asn Asn 
    1115                 1120                 1125             


Asn Asp  Pro Lys Lys Ala Phe  Thr Gly Ser Asn Ala  Leu Thr Lys 
    1130                 1135                 1140             


Lys Pro  Met Trp Phe Asp Glu  Gln Gln Thr Glu Lys  Val Pro Glu 
    1145                 1150                 1155             


Lys Val  Lys Thr Val Ser Phe  Glu Thr Ile Tyr Thr  Ile Arg Lys 
    1160                 1165                 1170             


Glu Ile  Ser Pro Asp Leu Lys  Ile Glu Lys Val Val  Asp Glu Lys 
    1175                 1180                 1185             


Val Arg  Gln Ile Leu Asn Asn  Arg Leu Asn Glu Tyr  Asn Gly Asn 
    1190                 1195                 1200             


Ala Lys  Gln Ala Phe Ser Asn  Leu Asp Glu Asn Pro  Ile Trp Leu 
    1205                 1210                 1215             


Asn Lys  Glu Lys Gly Ile Thr  Ile Lys Arg Val Thr  Ile Thr Gly 
    1220                 1225                 1230             


Ile Asn  Asn Ala Gln Ala Leu  His Glu Lys Leu Asp  Lys Asp Gly 
    1235                 1240                 1245             


Lys Pro  Ile Leu Asp Gly Glu  Gly Lys Lys Gln Pro  Val Asp Phe 
    1250                 1255                 1260             


Val Asn  Thr Gly Asn Asn His  His Val Ala Val Tyr  Cys Asp Ala 
    1265                 1270                 1275             


Asp Gly  Asn Leu Gln Glu Asn  Val Ile Ser Phe Tyr  Glu Ala Val 
    1280                 1285                 1290             


Thr Arg  Arg Asn Leu Gly Leu  Pro Ile Ile Asp Lys  Asp Tyr Lys 
    1295                 1300                 1305             


Lys Asp  Asp Gly Trp Gln Phe  Leu Phe Ser Met Lys  Gln Asn Glu 
    1310                 1315                 1320             


Tyr Phe  Val Phe Pro Asn Glu  Ser Thr Gly Phe Asn  Pro Asn Glu 
    1325                 1330                 1335             


Val Asp  Leu Leu Asn Pro Asp  Asn Tyr Ser Leu Ile  Ser Pro Asn 
    1340                 1345                 1350             


Leu Phe  Arg Val Gln Lys Phe  Thr Ile Lys Asp Tyr  Phe Phe Arg 
    1355                 1360                 1365             


His His  Leu Glu Thr Asn Val  Glu Ser Asn Ser Thr  Leu Lys Gly 
    1370                 1375                 1380             


Ile Thr  Trp Leu Arg Cys Gly  Leu Asn Asn Ile Asp  Asn Ile Ile 
    1385                 1390                 1395             


Lys Val  Arg Ile Asn His Ile  Gly Gln Ile Ile His  Val Gly Glu 
    1400                 1405                 1410             


Tyr 
    


<210>  1201
<211>  1415
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1201

Met Ala Lys Phe Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Val 
1               5                   10                  15      


Ser Ser Val Gly Tyr Ala Val Ile Thr Glu Asp Tyr Arg Val Pro Ala 
            20                  25                  30          


Phe Arg Phe Lys Val Leu Gly Asn Thr Glu Lys Glu Lys Ile Lys Lys 
        35                  40                  45              


Asn Leu Ile Gly Ser Thr Thr Phe Val Pro Ala Gln Ser Ala Gln Val 
    50                  55                  60                  


Thr Arg Val Phe Arg Val Asn Arg Arg Arg Ile Asp Arg Arg Asn His 
65                  70                  75                  80  


Arg Ile Ala Tyr Leu Arg Asp Ile Phe Gln Lys Glu Ile Glu Lys Val 
                85                  90                  95      


Asp Lys Asn Phe Tyr Arg Arg Leu Asp Glu Ser Phe Arg Val Leu Gly 
            100                 105                 110         


Asp Lys Ser Glu Asp Leu Gln Ile Lys Gln Pro Phe Phe Gly Asp Lys 
        115                 120                 125             


Glu Leu Glu Thr Ala Tyr His Lys Lys Tyr Pro Thr Ile Tyr His Leu 
    130                 135                 140                 


Arg Lys His Leu Ala Asp Ala Asp Lys Asn Ser Pro Val Ala Asp Ile 
145                 150                 155                 160 


Arg Glu Val Tyr Met Ala Leu Ser His Ile Leu Lys Tyr Arg Gly His 
                165                 170                 175     


Phe Leu Thr Leu Asp Lys Ile Asn Pro Asn Asn Ile Asn Met Gln Asn 
            180                 185                 190         


Ser Trp Ile Asn Phe Ile Glu Ser Cys Gln Glu Val Phe Asp Leu Glu 
        195                 200                 205             


Val Ser Glu Glu Ser Lys Thr Ile Ala Ala Ile Phe Lys Ser Ser Glu 
    210                 215                 220                 


Asn Arg Gln Glu Lys Val Lys Gln Ile Leu Ser Tyr Phe Gln Gln Glu 
225                 230                 235                 240 


Leu Leu Lys Lys Asp Lys Ser Ile Phe Lys Gln Leu Leu Gln Leu Leu 
                245                 250                 255     


Phe Gly Leu Lys Thr Lys Phe Lys Asp Cys Phe Glu Leu Glu Glu Glu 
            260                 265                 270         


Pro Asp Leu Asn Phe Ser Lys Glu Asn Tyr Asp Glu Asn Leu Glu Asn 
        275                 280                 285             


Leu Leu Gly Thr Leu Glu Glu Asp Phe Ser Asp Val Phe Ala Lys Leu 
    290                 295                 300                 


Lys Ile Leu Arg Asp Thr Ile Leu Leu Ser Asp Met Leu Thr Tyr Thr 
305                 310                 315                 320 


Gly Ala Thr His Ala Arg Phe Ser Ala Thr Met Val Glu Arg Tyr Glu 
                325                 330                 335     


Glu His Arg Lys Asp Leu Gln Arg Phe Lys Ser Phe Val Lys Gln Asn 
            340                 345                 350         


Leu Ser Glu Gln Asp Tyr Leu Asp Ile Phe Gly Arg Lys Thr Pro Asn 
        355                 360                 365             


Gly Phe Asp Val Asp Lys Glu Thr Lys Gly Tyr Val Gly Tyr Ile Ser 
    370                 375                 380                 


Asn Lys Met Val Leu Thr Asn Lys Gln Lys Thr Ile Gln Gln Asn Phe 
385                 390                 395                 400 


Tyr Asp Tyr Ile Ser Gly Lys Ile Thr Gly Ile Glu Gly Ala Glu Tyr 
                405                 410                 415     


Phe Leu Asn Lys Ile Ser Asp Gly Thr Phe Leu Arg Lys Leu Arg Thr 
            420                 425                 430         


Thr Asp Asn Gly Ala Ile Pro Asn Gln Ile His Ala Tyr Glu Leu Glu 
        435                 440                 445             


Lys Ile Ile Glu Arg Gln Gly Arg Asp Tyr Pro Phe Leu Leu Glu Asn 
    450                 455                 460                 


Lys Asp Lys Leu Leu Ser Ile Leu Thr Phe Lys Ile Pro Tyr Tyr Val 
465                 470                 475                 480 


Gly Pro Leu Ala Lys Gly Asn Asn Ser Arg Phe Ala Trp Ile Lys Arg 
                485                 490                 495     


Ala Thr Ser Gln Asp Ile Leu Asp Asp Asn Asp Glu Asp Thr Arg Asn 
            500                 505                 510         


Gly Lys Ile Arg Pro Trp Asn Tyr His Lys Leu Ile Asn Met Asp Lys 
        515                 520                 525             


Thr Arg Asp Ala Phe Ile Thr Asn Leu Ile Gly Asn Asp Ile Ile Leu 
    530                 535                 540                 


Leu Asn Glu Lys Val Leu Pro Lys Arg Ser Leu Ile Tyr Glu Glu Val 
545                 550                 555                 560 


Met Leu Gln Asn Glu Leu Thr Arg Val Lys Tyr Lys Asp Lys Tyr Gly 
                565                 570                 575     


Lys Thr His Phe Phe Asp Ser Glu Leu Arg Gln Glu Ile Ile Asn Asn 
            580                 585                 590         


Leu Phe Lys Thr Asn Ser Lys Arg Val Ser Ser Ala Met Leu Leu Asn 
        595                 600                 605             


Tyr Leu Glu Asn Tyr Thr Asn Leu Gln Ala Val Glu Ile Val Ser Gly 
    610                 615                 620                 


Ile Glu Lys Gly Lys Ser Leu Asn Ser Thr Leu Lys Thr Tyr Asn Asp 
625                 630                 635                 640 


Leu Lys Thr Ile Phe Ser Glu Glu Leu Leu Asp Ser Glu Ile Tyr Gln 
                645                 650                 655     


Lys Glu Leu Glu Glu Ile Val Lys Val Ile Thr Val Phe Asp Asp Lys 
            660                 665                 670         


Lys Ser Ile Lys Asn Tyr Leu Thr Lys Tyr Phe Gly Arg Leu Glu Phe 
        675                 680                 685             


Leu Asn Glu Glu Arg Ile Asn Gln Leu Ser Lys Leu Arg Tyr Thr Gly 
    690                 695                 700                 


Trp Gly Arg Tyr Ser Ala Lys Leu Leu Leu Asp Ile Arg Asp Glu Asp 
705                 710                 715                 720 


Thr Gly Phe Asn Leu Met Gln Phe Leu Arg Asn Asp Asp Glu Asn Arg 
                725                 730                 735     


Asn Leu Ser Gln Leu Ile Ser Asp Ser Thr Leu Ser Phe Asp Ser Ala 
            740                 745                 750         


Ile Lys Ser Ile Gln Ser Lys Ser Thr Val Glu Asp Asp Val Phe Asp 
        755                 760                 765             


Glu Ile Lys Lys Leu Ala Gly Ser Pro Ala Ile Lys Arg Gly Ile Leu 
    770                 775                 780                 


Asn Ser Ile Lys Ile Val Asp Glu Leu Val Gln Ile Ile Gly Tyr Pro 
785                 790                 795                 800 


Pro Gln Asn Ile Val Ile Glu Met Ala Arg Glu Asn Met Thr Thr Glu 
                805                 810                 815     


Glu Gly Gln Lys Lys Ala Lys Thr Arg Lys Thr Lys Leu Glu Ser Ala 
            820                 825                 830         


Leu Lys Asn Ile Glu Asn Ser Val Leu Glu Asn Gly Lys Val Pro His 
        835                 840                 845             


Ser Asp Glu Gln Leu Gln Ser Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
    850                 855                 860                 


Asn Gly Lys Asp Met Tyr Ser Leu Asp Lys Thr Gly Ser Pro Thr Pro 
865                 870                 875                 880 


Leu Tyr Arg Asp Arg Leu Asp Gln Tyr Glu Val Asp His Ile Ile Pro 
                885                 890                 895     


Tyr Ser Phe Leu Pro Ile Asp Ser Ile Asp Asn Lys Val Leu Thr His 
            900                 905                 910         


Arg Glu Asn Asn Gln Arg Lys Leu Asn Asn Ile Pro Asp Lys Glu Thr 
        915                 920                 925             


Val Ala Asn Met Lys Pro Phe Trp Glu Lys Leu Tyr Asn Ala Lys Leu 
    930                 935                 940                 


Ile Ser Gln Thr Lys Tyr Gln Arg Leu Thr Thr Ser Glu Arg Thr Pro 
945                 950                 955                 960 


Asp Gly Val Leu Thr Glu Ser Met Lys Ala Gly Phe Ile Glu Arg Gln 
                965                 970                 975     


Leu Val Glu Thr Arg Gln Ile Ile Lys His Val Ala Arg Ile Leu Asp 
            980                 985                 990         


Asn Arg Phe Leu Asp Thr Lys Ile  Ile Thr Leu Lys Ser  Gln Leu Ile 
        995                 1000                 1005             


Thr Asn  Phe Arg Asn Thr Phe  His Ile Ala Lys Ile  Arg Glu Leu 
    1010                 1015                 1020             


Asn Asp  Tyr His His Ala His  Asp Ala Tyr Leu Ala  Val Val Ile 
    1025                 1030                 1035             


Gly Gln  Thr Leu Leu Lys Ala  Tyr Pro Lys Leu Ala  Pro Glu Leu 
    1040                 1045                 1050             


Ile Tyr  Gly His His Ala His  Phe Asn Arg His Glu  Glu Asn Lys 
    1055                 1060                 1065             


Ala Thr  Ile Arg Lys His Leu  Tyr Ser Asn Ile Met  Arg Phe Phe 
    1070                 1075                 1080             


Asn Asn  Pro Asn Ser Lys Val  Ser Lys Asp Ile Trp  Asn Cys Asn 
    1085                 1090                 1095             


Arg Asp  Leu Pro Ile Ile Lys  Asp Ile Ile Tyr Asn  Ser Gln Val 
    1100                 1105                 1110             


Asn Phe  Val Lys Arg Thr Met  Ile Lys Lys Gly Ala  Phe Tyr Lys 
    1115                 1120                 1125             


Gln Asn  Ile Ile Pro Asn Asp  Ser Ser Val Thr Val  Asp Lys Arg 
    1130                 1135                 1140             


Phe Pro  Ile Lys Asn Lys Lys  Tyr Leu Asp Pro Lys  Leu Tyr Gly 
    1145                 1150                 1155             


Gly Tyr  Tyr Glu Arg Asn Ser  Ala Leu Ser Val Phe  Ile Ile Gly 
    1160                 1165                 1170             


Gln Ser  Phe Asn Lys Asn Asn  Lys Leu Lys Val Val  Lys Glu Phe 
    1175                 1180                 1185             


Tyr Asp  Ile Asn Ile Ile Asp  Tyr Pro Leu Phe Asn  Thr Asp Pro 
    1190                 1195                 1200             


Met Ser  Phe Phe Asn Asp Asp  Ser Glu Asn Gly Phe  Leu Thr Lys 
    1205                 1210                 1215             


Asn Asn  Leu Gln Ser Val Gln  Ala Phe Tyr Lys Ile  Pro Lys Tyr 
    1220                 1225                 1230             


Ser Leu  Ile Gln Lys Asn Asp  Gly Thr Arg Met Leu  Phe Glu Ser 
    1235                 1240                 1245             


Thr Thr  Asn Phe His Lys Ala  Thr Gln Phe Lys Leu  Thr Glu Ala 
    1250                 1255                 1260             


Gln Asn  Glu Leu Phe Phe His  Met Lys Arg Leu Leu  Thr Lys Thr 
    1265                 1270                 1275             


Asp Phe  Met Asp Leu Lys Ser  Glu Glu Ser Ile Lys  Glu Ser Gln 
    1280                 1285                 1290             


Asp Phe  Ile Met Asn His Lys  Asn Glu Phe Asp Asn  Ile Val Asn 
    1295                 1300                 1305             


Gly Leu  Phe Gly Tyr Ala Glu  Arg Gln Leu Gly Ser  Lys Ser Leu 
    1310                 1315                 1320             


Phe Thr  Gln Ala His Glu Ala  Tyr Lys Leu Arg Lys  Tyr Pro Val 
    1325                 1330                 1335             


Phe Ser  Leu Asn Glu Glu Thr  Ile Arg Tyr Tyr Tyr  Asp Asn Phe 
    1340                 1345                 1350             


Leu Arg  Leu Phe Ser Leu Thr  Lys Ser Gly Thr Ser  Ser Gly Tyr 
    1355                 1360                 1365             


Val Val  Gly Leu Phe Glu Ala  Pro Lys Leu Arg Asn  Val Val Tyr 
    1370                 1375                 1380             


Arg Ala  Thr Lys Asp Glu Ser  Asn Ala Thr Leu Ile  His Gln Ser 
    1385                 1390                 1395             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Lys Leu Gly 
    1400                 1405                 1410             


Glu Asp  
    1415 


<210>  1202
<211>  1415
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1202

Met Asn Leu Arg Asn Val Ala Lys Glu Gly Ile Asn Tyr Asn Ile Gly 
1               5                   10                  15      


Leu Asp Leu Gly Thr Gly Ser Val Gly Trp Ala Val Thr Asp Glu Gln 
            20                  25                  30          


Gly Glu Leu Phe Ser Phe Lys Gly Lys Thr Thr Trp Gly Ser Arg Ile 
        35                  40                  45              


Phe Pro Thr Ala Glu Thr Ala Ala Asp Thr Arg Leu Lys Arg Gly Gln 
    50                  55                  60                  


Arg Arg Arg Tyr Ala Arg Arg Arg Trp Arg Leu Asp Leu Leu Gln Ser 
65                  70                  75                  80  


Leu Phe Glu Glu Glu Ile Lys Lys Val Asp Pro Glu Phe Phe Thr Arg 
                85                  90                  95      


Leu Arg Gln Ser His Leu Leu Lys Glu Asp Lys Thr Tyr Ala Ala Ile 
            100                 105                 110         


Leu Ser Ser Lys Gly Ala Thr Arg Pro Leu Phe Pro Glu Gly Glu Ala 
        115                 120                 125             


Glu Val Glu Tyr Tyr Lys Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys 
    130                 135                 140                 


Ala Leu Met Glu Ser Asn Asn Gln Glu Asp Ile Arg Leu Ile Tyr Leu 
145                 150                 155                 160 


Ala Phe His Asn Ile Val Lys Ala Arg Gly Asn Phe Leu His Gln Asp 
                165                 170                 175     


Thr Pro Gly Leu Thr Ala Ser Asn Ala Asn Met Gly Asp Ala Val Glu 
            180                 185                 190         


Lys Leu Cys Ser Ala Leu Glu Asp Trp Cys Ser Ser Gln Asn Ile Glu 
        195                 200                 205             


Cys Ala Ile Arg Lys Asn Gln Lys Ser Ile Gln Asp Leu Met Glu Gly 
    210                 215                 220                 


Lys Thr Ile Glu Pro Ser Glu Ser Asn Ala Leu Lys Glu Thr Phe Asp 
225                 230                 235                 240 


Ala Ser Arg Ser Ser Arg Lys Asp Lys Ala Ser Val Leu Ala Gly Trp 
                245                 250                 255     


Ile Ala Ile Gln Pro Gly Glu Gln Phe Glu Pro Lys Gln Ala Lys Asp 
            260                 265                 270         


Ala Ser Lys Glu Ile Ala Asn Ala Val Phe Gly Leu Ser Ala Asn Met 
        275                 280                 285             


Lys Lys Val Phe Val Glu Leu Glu Glu Asp Gln Lys Ile Tyr Leu Asn 
    290                 295                 300                 


Asn Asp Glu Gln Val Glu Ala Phe Tyr Thr Val Cys Pro Asp Glu Gly 
305                 310                 315                 320 


Val Ala Leu Phe Glu Ala Leu Gln Ser Met Tyr Ser Ala Tyr Val Leu 
                325                 330                 335     


Met Gly Ile Leu Lys Gly Ala Asp Gly Gly Ser Ile Ser Ser Cys Lys 
            340                 345                 350         


Val Ala Ala Tyr Glu Gln Tyr Lys Glu Asp Leu Lys Leu Leu Lys Thr 
        355                 360                 365             


Leu Val Arg Glu Phe Ala Pro Lys Lys Tyr Asp Glu Phe Phe Arg Gly 
    370                 375                 380                 


Asp Ile Phe Asp Ala Lys Lys Gly Phe Arg Thr Arg Asn Tyr Asp Pro 
385                 390                 395                 400 


Ala Thr Ala Lys Gly Tyr Thr Lys Tyr Asn Ser Val Arg Gly Ala Ser 
                405                 410                 415     


Tyr Asp Ala Phe Lys Lys Glu Val Glu Lys Leu Phe Glu Gly Thr Ala 
            420                 425                 430         


Ala Glu Glu Asp Val Arg Tyr Thr Asp Met Met Gln Arg Phe Asp Glu 
        435                 440                 445             


Gly Thr Phe Leu Arg Arg Leu Lys Thr Ser Asp Asn Gly Ser Ile Pro 
    450                 455                 460                 


Tyr Gln Leu His Leu Glu Glu Met Val Ala Ile Ile Glu Asn Gln Lys 
465                 470                 475                 480 


Thr Phe Tyr Pro Phe Leu Glu Glu Asn Lys Glu Lys Phe Glu Ser Leu 
                485                 490                 495     


Val Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Thr Gln Lys Asn 
            500                 505                 510         


Ala Arg Pro Asp Asn His Gly Asn Ala Arg Phe Ala Trp Ser Gln Arg 
        515                 520                 525             


Lys Glu Gly Gln Glu Asn Ala Arg Val Tyr Pro Trp Asn Trp Glu Glu 
    530                 535                 540                 


Val Ile Asp Arg Lys Gln Ser Ala Ala Lys Phe Met Glu Arg Leu Thr 
545                 550                 555                 560 


Gly Thr Cys Thr Tyr Ile Leu Glu Glu Pro Val Leu Pro Arg Tyr Ser 
                565                 570                 575     


Leu Leu Tyr Glu Glu Tyr Cys Leu Leu Asn Glu Leu Asn Gly Ala Arg 
            580                 585                 590         


Trp Arg Asp Met Gly Gly Asp Trp His Arg Phe Asp Tyr Glu Thr Arg 
        595                 600                 605             


Met Asp Ile Ile Glu Asp Leu Phe Lys Lys Lys Lys Gly Thr Val Lys 
    610                 615                 620                 


Tyr Ala Thr Val Ala Asp Trp Leu Val Arg Glu Lys His Arg Ser Cys 
625                 630                 635                 640 


Ala Glu Val Lys Gly Gly Gln Gly Glu Thr Gly Phe Glu Ser Lys Leu 
                645                 650                 655     


Ser Ser Tyr Val Phe Phe Lys Gly Val Leu His Val Asp Glu Ile Asp 
            660                 665                 670         


Glu Ala Asp Ile Pro Met Ile Glu Glu Leu Ile Trp Trp Asn Thr Leu 
        675                 680                 685             


Phe Glu Asp Arg Ser Ile Leu Lys Glu Ser Ile Glu Asp Lys Tyr Gly 
    690                 695                 700                 


Asp Arg Leu Asn Asp Glu Gln Ile Lys Ala Ile Cys Lys Lys Arg Phe 
705                 710                 715                 720 


Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Leu Cys Gly Ile Lys Ala 
                725                 730                 735     


Gln Thr Asp Asn Gly Pro Cys Ser Ile Met Asp Val Leu Arg Glu Gly 
            740                 745                 750         


Asn Pro Asn Asn Asp Ala Arg Ser Ala Thr Met Val Phe Met Glu Ile 
        755                 760                 765             


Ile Arg Asp Asp Lys Phe Gly Phe Thr Lys Leu Ile Glu Glu Glu Asn 
    770                 775                 780                 


Lys Lys Arg Ile Ser Ser Val Asp Asp Phe Asp Val Asn Asp Leu Pro 
785                 790                 795                 800 


Gly Ser Pro Ala Val Arg Arg Thr Val Asn Gln Ala Lys Arg Ile Val 
                805                 810                 815     


Asp Glu Ile Val Gly Ile Ala Gly Cys Ala Pro Gln Asn Ile Phe Ile 
            820                 825                 830         


Glu Val Thr Arg Asp Glu Asp Gly Arg Asn Lys Gly Arg Arg Thr Lys 
        835                 840                 845             


Arg Arg Tyr Asp Ala Ile Gln Glu Ala Val Lys Val Leu Lys Lys Glu 
    850                 855                 860                 


Gln Lys Glu Tyr Phe Asp Ala Asn Val Ala Asp Leu Leu Lys Asn Thr 
865                 870                 875                 880 


Ala Pro Ala Ala Leu Asp Asp Glu Arg Leu Ala Leu Tyr Phe Met Gln 
                885                 890                 895     


Asn Gly Lys Cys Met Tyr Cys Gly Lys Asp Leu Asp Ile Asn Gln Leu 
            900                 905                 910         


Asp Lys Tyr Gln Val Asp His Ile Leu Pro Gln Ser Tyr Val Lys Asp 
        915                 920                 925             


Asp Ser Phe Glu Asn Lys Ala Leu Val His Ser Asp Asp Asn Gln Arg 
    930                 935                 940                 


Lys Ser Asn Ser Met Leu Leu Asp Asp Ser Ile Ile Arg Lys Met Lys 
945                 950                 955                 960 


Pro Tyr Trp Glu Ala Leu Arg Ser Ala Gly Leu Ile Gly Glu Lys Lys 
                965                 970                 975     


Phe Lys Asn Leu Thr Arg Val Arg Val Ser Asp Lys Glu Leu Gly Gly 
            980                 985                 990         


Phe Ile Ala Arg Gln Met Val Glu  Thr Ser Gln Ser Met  Lys Leu Thr 
        995                 1000                 1005             


Ser Leu  Leu Leu Glu Gln Ala  Tyr Pro Glu Thr Gln  Val Arg Pro 
    1010                 1015                 1020             


Val Lys  Ala Gly Phe Ser Ser  Asp Leu Arg Arg Arg  Leu Lys Leu 
    1025                 1030                 1035             


Val Lys  Cys Arg Glu Ile Asn  Asp Tyr His His Ala  His Asp Ala 
    1040                 1045                 1050             


Tyr Leu  Ala Cys Glu Val Gly  Gln Phe Ile Ala Leu  Arg His Asn 
    1055                 1060                 1065             


Lys Leu  Tyr Glu Asn Pro Ile  Gly Tyr Ser His Ala  Val Lys Asp 
    1070                 1075                 1080             


Phe Ile  Ala Lys Arg Ala Lys  Tyr Ala Ser Glu His  Gly Lys Pro 
    1085                 1090                 1095             


Met Asp  Thr Tyr Gly Met Pro  Gly Ser Ser Ser Phe  Val Ile Ser 
    1100                 1105                 1110             


Ser Phe  Met Thr Ser Gly Phe  Asp Glu Glu Thr Gly  Glu Ile Phe 
    1115                 1120                 1125             


Lys Asp  Ala Trp Asp Ala Ser  Leu Ala Ala Val Arg  Ile His Gln 
    1130                 1135                 1140             


Ala Leu  Asn Arg Lys Asp Cys  Tyr Ile Ser Arg Met  Pro Glu Glu 
    1145                 1150                 1155             


Thr Ser  Gly Ala Phe Trp Asp  Ala Thr Ile Tyr Ser  Pro Lys Gln 
    1160                 1165                 1170             


Thr Lys  Lys Lys Leu Ser Leu  Pro Leu Lys Lys Gly  Leu Asp Pro 
    1175                 1180                 1185             


Gln Lys  Tyr Gly Ser Tyr Ser  Arg Glu Gln Phe Ala  Tyr Phe Phe 
    1190                 1195                 1200             


Val Tyr  Glu Ala Lys Lys Ile  Lys Lys Lys Glu Glu  Thr Lys Glu 
    1205                 1210                 1215             


Tyr Val  Phe Ala Ser Val Pro  Val Gln Val Ala Ala  Leu Val Ala 
    1220                 1225                 1230             


Ala Asp  Pro Asn Ala Leu Ile  Glu Phe Ala Lys Ala  Asp Ala Glu 
    1235                 1240                 1245             


Glu Arg  Gly Leu Lys Phe Ala  Lys Ile Val Arg Glu  Lys Val Tyr 
    1250                 1255                 1260             


Lys Tyr  Gln Leu Ile Glu Val  Asn Asp Glu Arg Phe  Phe Val Thr 
    1265                 1270                 1275             


Gly Leu  Arg Thr Val Arg Asn  Gly Leu Gln Ile Ala  Phe Ser Glu 
    1280                 1285                 1290             


Gln Asp  Arg Asn Ile Val Ser  Gly Ile Val Glu Gly  Asn Ser Ser 
    1295                 1300                 1305             


Asn Asn  Asn Leu Leu Asp Ile  Tyr Asp Lys Ile Thr  Asn Arg Tyr 
    1310                 1315                 1320             


Ala Leu  Tyr Ala Ser Arg Phe  Gly Lys Leu Leu Asn  Leu Ser Gly 
    1325                 1330                 1335             


Tyr Arg  Glu Asn Phe Ile Gln  Ala Ser Leu Glu Asp  Gln Glu Arg 
    1340                 1345                 1350             


Val Val  Leu Ala Leu Ile Ser  Leu Phe Asn Ala Arg  Thr Asn Met 
    1355                 1360                 1365             


Ile Asp  Leu Ser Ala Ile Gly  Ser Ser Lys Thr Val  Gly Asn Met 
    1370                 1375                 1380             


Ser Met  Thr Phe Ala Lys Glu  Met Ala Met Ala Asp  Asn Arg Phe 
    1385                 1390                 1395             


Ala Phe  Ile Asp Gln Ser Val  Thr Gly Met Leu Glu  Arg Lys Thr 
    1400                 1405                 1410             


Tyr Leu  
    1415 


<210>  1203
<211>  1416
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1203

Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Glu Phe 
1               5                   10                  15      


Gly Lys Gly Asn Ser Val Ser Gln Thr Ser Glu Arg Thr Ala Tyr Arg 
            20                  25                  30          


Ser Thr Arg Arg Leu Arg Glu Arg Phe Leu Leu Arg Arg Glu Arg Leu 
        35                  40                  45              


His Arg Val Leu Asn Ile Leu Asp Phe Leu Pro Thr His Tyr Ala Ser 
    50                  55                  60                  


Gln Ile Asp Phe Lys Lys Arg Leu Gly Lys Phe Lys Glu Asn Lys Glu 
65                  70                  75                  80  


Pro Lys Ile Ala Tyr Asp Asn Lys Ser Phe Leu Phe Leu Asp Ser Phe 
                85                  90                  95      


Asn Glu Met Leu Lys Asp Phe Glu Gln Glu Gln Pro Asn Met Leu Ile 
            100                 105                 110         


Asn Glu Lys Gly Glu Lys Lys Leu Ile Pro Tyr Asp Trp Thr Leu Tyr 
        115                 120                 125             


Tyr Leu Arg Lys Lys Ala Leu Arg Glu Lys Ile Ser Lys Glu Glu Leu 
    130                 135                 140                 


Ser Trp Ile Ile Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu 
145                 150                 155                 160 


Arg Gly Glu Asp Glu Glu Glu Asn Pro Ser Lys Leu Val Glu Phe His 
                165                 170                 175     


Ser Leu Thr Val Val Asp Val Val Pro Asp Glu Pro Ile Lys Gly Lys 
            180                 185                 190         


Leu Glu Thr Trp Tyr Ser Ile Ile Leu Asp Asn Gly Trp Val Tyr Arg 
        195                 200                 205             


Arg Ser Ser Lys Thr Glu Leu Tyr Asp Trp Lys Asp Lys Ile Lys Glu 
    210                 215                 220                 


Phe Ile Val Thr Thr Asp Leu Asn Tyr Asp Gly Thr Val Lys Thr Asp 
225                 230                 235                 240 


Lys Glu Gly Asn Val Lys Arg Ser Phe Arg Ala Pro Ser Glu Thr Asp 
                245                 250                 255     


Trp Thr Leu Ile Lys Lys Lys Thr Glu Tyr Glu Ile Glu Gln Ser Ser 
            260                 265                 270         


Lys Thr Ile Gly Glu Tyr Ile Tyr Asn Thr Leu Leu Lys Tyr Pro Glu 
        275                 280                 285             


Gln Lys Ile Arg Gly Lys Leu Ile Arg Thr Ile Glu Arg Lys Tyr Tyr 
    290                 295                 300                 


Lys Glu Glu Leu Lys Gln Ile Leu Leu Lys Gln Thr Glu Phe His Asn 
305                 310                 315                 320 


Glu Leu Lys Asp Asp Ser Leu Tyr Tyr Asp Cys Leu Arg Glu Leu Tyr 
                325                 330                 335     


Arg Asn Asn Glu Ser His Gln Leu Phe Leu Glu Lys Lys Asn Phe Ala 
            340                 345                 350         


Gln Leu Phe Ile Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser 
        355                 360                 365             


Gln Lys Ser Leu Ile Ser Asn Cys Pro Leu Glu Phe Tyr Lys Phe Lys 
    370                 375                 380                 


Asp Ser Val Gly Asn Glu Lys Val Gln His Leu Lys Ala Ile Pro Lys 
385                 390                 395                 400 


Ser Asn Pro Phe Tyr Gln Glu Phe Arg Ile Trp Gln Trp Ile Ser Asn 
                405                 410                 415     


Leu Ser Ile Tyr Ser Lys Leu Asp Asp Ser Asn Phe Thr Ser His Phe 
            420                 425                 430         


Phe Asp Ser Thr Asp Asp Trp Glu Ser Leu Phe Glu Met Leu Asn Gly 
        435                 440                 445             


Arg Lys Glu Ile Glu Gln Lys Gln Leu Leu Lys Phe Leu Leu Glu Lys 
    450                 455                 460                 


Lys Gly Phe Lys Gly Lys Ser Leu Ser Ser Glu Ile Glu Lys Tyr Arg 
465                 470                 475                 480 


Trp Asn Tyr Val Glu Asp Lys Lys Tyr Pro Cys Asn Glu Thr Arg Ser 
                485                 490                 495     


Leu Ile Gln Ser Arg Leu Asp Lys Val Ala Asn Leu Pro Glu Ser Phe 
            500                 505                 510         


Leu Thr Arg His Val Glu Gln Glu Leu Trp His Leu Ile Tyr Ser Val 
        515                 520                 525             


Thr Asp Lys Ile Glu Phe Glu Lys Ala Leu Asp Thr Phe Ser Lys Lys 
    530                 535                 540                 


Tyr Lys Ile Asp Thr Pro Thr Phe Val Asp Ala Phe Lys Lys Cys Pro 
545                 550                 555                 560 


Pro Phe Lys Ser Glu Tyr Gly Ser Phe Ser Glu Lys Ala Ile Lys Lys 
                565                 570                 575     


Leu Leu Pro Leu Met Arg Thr Gly Lys Tyr Trp Asp Leu Asp Asn Ile 
            580                 585                 590         


Asp Lys Ala Thr Lys Gln Arg Ile Glu Lys Ile Ile Ser Gly Glu Tyr 
        595                 600                 605             


Asp Lys Asp Ile Arg Asp Arg Val Arg Glu Lys Ala Ile Asn Leu Leu 
    610                 615                 620                 


Ser Glu Thr Asp Phe Asn Arg Leu Gln Leu Trp Leu Ala Gln Tyr Ile 
625                 630                 635                 640 


Val Tyr Asp Arg His Ser Glu Ala Thr Ser Leu Asp Lys Trp Glu Thr 
                645                 650                 655     


Ile Asp Asp Ile Val Thr Tyr Leu His Ser Phe Lys Gln His Ser Leu 
            660                 665                 670         


Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Ile Arg Val Val 
        675                 680                 685             


Lys Asp Ile Trp Ser Asn Tyr Gly Asn Gly Ser Lys Asp Tyr Phe Asn 
    690                 695                 700                 


Glu Ile His Val Glu Leu Gly Arg Glu Met Lys Leu Pro Ala Glu Glu 
705                 710                 715                 720 


Arg Lys Arg Ile Gln Glu Lys Asn Ser Gln Asn Glu Asp Thr Asn Leu 
                725                 730                 735     


Arg Ile Lys Ala Leu Leu Ser Glu Met Gln Asn Asp Pro Asn Val Glu 
            740                 745                 750         


Asn Val Arg Pro Tyr Ser Pro Val Gln Gln Glu Ile Leu Lys Ile Tyr 
        755                 760                 765             


Glu Glu Gly Val Leu Asn Ser Asn Ile Glu Ile Ala Asp Asp Ile Leu 
    770                 775                 780                 


Lys Ile Ser Lys Thr Ala Gln Pro Ser Ser Ser Asp Leu Lys Arg Tyr 
785                 790                 795                 800 


Lys Leu Trp Leu Glu Gln Lys Tyr Gln Ser Pro Tyr Thr Gly Glu Ile 
                805                 810                 815     


Ile Pro Leu Asn Lys Leu Phe Thr Pro Glu Tyr Glu Ile Glu His Ile 
            820                 825                 830         


Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Tyr Asn Asn Lys Val Ile 
        835                 840                 845             


Cys Glu Ser Ala Val Asn Lys Leu Lys Asp Asn Ser Leu Gly Tyr Glu 
    850                 855                 860                 


Phe Ile Lys Gly Tyr Asn Gly Gln Ile Val Glu Cys Gly Phe Gly Lys 
865                 870                 875                 880 


Lys Val Lys Ile Phe Ser Ile Asp Glu Tyr Glu Asp Phe Val Gln Lys 
                885                 890                 895     


Asn Tyr Ala Asn Asn Arg Ser Lys Ala Ser Arg Leu Met Met Ala Asp 
            900                 905                 910         


Ile Pro Glu Lys Met Ile Glu Arg Gln Leu Asn Asp Thr Arg Tyr Ile 
        915                 920                 925             


Ser Lys Tyr Ile Ser Asn Ile Leu Ser Asn Ile Val Arg Ser Glu Ser 
    930                 935                 940                 


Asn Asp Asp Gly Val Asn Ser Lys Asn Leu Ile Ser Val Asn Gly Lys 
945                 950                 955                 960 


Ile Thr Thr Val Leu Lys Lys Asp Trp Gly Met Asn Asp Ile Trp Asn 
                965                 970                 975     


Asp Leu Ile Leu Pro Arg Phe Ile Arg Met Asn Gln Ile Thr Asp Ser 
            980                 985                 990         


Asp Ser Phe Thr Thr Trp Asn Glu  Arg Phe Gln Lys Phe  Ile Pro Thr 
        995                 1000                 1005             


Val Pro  Ile Glu Leu Ser Lys  Asn Phe Ser Ala Lys  Arg Ile Asp 
    1010                 1015                 1020             


His Arg  His His Ala Met Asp  Ala Leu Val Ile Ala  Cys Ala Thr 
    1025                 1030                 1035             


Arg Asp  His Val Asn Leu Leu  Asn Asn Leu Tyr Ala  Lys Ser Ser 
    1040                 1045                 1050             


Ala Arg  Tyr Asp Leu Asn Arg  Lys Leu Arg Ile Phe  Glu Lys Thr 
    1055                 1060                 1065             


Thr Tyr  Lys Asp Ile Lys Thr  Gly Gln Ile Ile Glu  Arg Glu Ile 
    1070                 1075                 1080             


Pro Lys  Ala Phe Leu Lys Pro  Trp Gln Asn Phe Thr  Thr Asp Ala 
    1085                 1090                 1095             


Lys Asp  Ala Ile Glu Ser Ile  Ile Val Ser Tyr Lys  Gln Ser Leu 
    1100                 1105                 1110             


Arg Val  Ile Asn Lys Ser Thr  Asn Tyr Tyr Gln Lys  Trp Val Glu 
    1115                 1120                 1125             


Lys Asp  Gly Ile Val Lys Lys  Glu Lys Ile Lys Gln  Glu Gly Lys 
    1130                 1135                 1140             


Asn Trp  Ala Ile Arg Lys Pro  Leu His Lys Asp Thr  Val Ser Gly 
    1145                 1150                 1155             


Leu Val  Gln Leu Ser Trp Val  Lys Val Pro Lys Gly  Lys Val Met 
    1160                 1165                 1170             


Thr Ala  Thr Arg Lys Ser Leu  Asp Ser Ser Phe Asp  Leu Lys Thr 
    1175                 1180                 1185             


Ile Ala  Thr Ile Thr Asp Thr  Gly Ile Gln Lys Ile  Leu Ile Lys 
    1190                 1195                 1200             


Tyr Leu  Glu Ser Lys Gly Asn  Asn Pro Glu Ser Ala  Phe Ser Pro 
    1205                 1210                 1215             


Glu Gly  Ile Glu Asp Phe Asn  Asn Asn Ile His Leu  Tyr Asn Asp 
    1220                 1225                 1230             


Gly Lys  Lys His Arg Pro Ile  Thr Lys Val Arg Leu  Tyr Glu Ile 
    1235                 1240                 1245             


Gly Ser  Lys Phe Pro Leu Gly  Glu Lys Gly Asn Lys  Asn Ala Lys 
    1250                 1255                 1260             


Tyr Val  Glu Ala Ala Lys Gly  Thr Asn Leu Phe Phe  Ala Val Tyr 
    1265                 1270                 1275             


Ala Asn  Asn Lys Gly Glu Arg  Ser Phe Glu Thr Ile  Pro Leu Asn 
    1280                 1285                 1290             


Leu Val  Ile Asp Arg Leu Lys  Leu Gly Leu Ser Ala  Val Pro Glu 
    1295                 1300                 1305             


Thr Asn  Glu Lys Gly Asp Asn  Leu Leu Phe His Leu  Ser Pro Asn 
    1310                 1315                 1320             


Asp Leu  Val Tyr Val Pro Ser  Glu Asp Glu Met Glu  Asn Leu Lys 
    1325                 1330                 1335             


Leu Ile  Asn Leu Glu His Ile  Ser Lys Asp Gln Leu  Asn Arg Ile 
    1340                 1345                 1350             


Tyr Lys  Met Val Ser Ser Thr  Ser Asn Arg Cys Phe  Phe Ile Lys 
    1355                 1360                 1365             


Ser Glu  Ile Ala Leu Ser Ile  Val Asn Lys Ile Glu  Tyr Ser Pro 
    1370                 1375                 1380             


Leu Asn  Lys Met Glu Lys Ser  Ile Glu Gly Ile Met  Ile Lys Asp 
    1385                 1390                 1395             


Val Cys  Leu Lys Leu Gln Val  Asn Arg Ile Gly Asn  Leu Glu Arg 
    1400                 1405                 1410             


Tyr Phe  Ser 
    1415     


<210>  1204
<211>  1418
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1204

Met Lys Val Lys Asn Ile Leu Gly Leu Asp Ile Gly Val Thr Ser Ile 
1               5                   10                  15      


Gly Phe Ala His Val Thr Glu Asn Glu Lys Asp Asn Lys Ser His Ile 
            20                  25                  30          


Lys Gln Leu Gly Val Arg Ile Asn Pro Leu Thr Val Asp Glu Gln Thr 
        35                  40                  45              


Asn Phe Glu Lys Gly Arg Pro Ala Ser Val Asn Ala Asp Arg Thr Leu 
    50                  55                  60                  


Lys Arg Gly Ala Arg Lys Met Leu Ser Arg Tyr Lys Asp Arg Arg Lys 
65                  70                  75                  80  


Asn Leu Ile Glu Ile Leu Ser Lys Thr Gly Phe Ile Asn Ser Glu Ser 
                85                  90                  95      


Ile Leu Ala Glu Asp Gly Lys Asn Thr Thr His Ser Thr Trp Ala Leu 
            100                 105                 110         


Arg Ala Lys Ala Ser Arg Glu Arg Ile Glu Lys Asp Glu Leu Gly Arg 
        115                 120                 125             


Val Leu Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys 
    130                 135                 140                 


Ala Lys Asn Glu Asp Glu Gly Gln Ala Ile Asp Gly Met Ala Ile Ala 
145                 150                 155                 160 


Lys Arg Leu Tyr Glu Glu Asn Leu Thr Pro Gly Gln Leu Val Tyr Gln 
                165                 170                 175     


Leu Leu Arg Glu Gly Lys Lys Tyr Ile Pro Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         


Leu Gln Asp Glu Leu Asp Lys Val Trp Asn Phe Gln Lys Gln Phe His 
        195                 200                 205             


Gln Glu Ile Leu Thr Asp Thr Phe Tyr Lys Asp Ile Gln Gly Lys Gly 
    210                 215                 220                 


Gln Arg Ala Thr Ser Ala Ser Phe Tyr Gly Val Tyr Gly Phe Asn Thr 
225                 230                 235                 240 


Ala Glu Asn Lys Gly Ser Arg Glu Glu Lys Arg Leu Gln Ala Tyr Ala 
                245                 250                 255     


Trp Arg Ser Asp Ala Leu Thr Lys Gln Leu Thr Arg Glu Glu Val Ala 
            260                 265                 270         


Phe Val Ile Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr 
        275                 280                 285             


Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Phe Phe Asn Lys Lys 
    290                 295                 300                 


Thr Val Gly Gln Tyr Leu Tyr Glu Gln Leu Gln Gln Asn Pro His Thr 
305                 310                 315                 320 


Arg Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe 
                325                 330                 335     


Glu Thr Ile Trp Thr Ile Gln Ser Arg Tyr Tyr Pro Glu Leu Thr Asp 
            340                 345                 350         


Asp Leu Lys Arg Glu Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Gly Leu Val Ser Phe Cys Glu Leu Glu Ser Lys 
    370                 375                 380                 


Glu Ile Met Ile Glu Lys Asn Gly Lys Ala Ala Val Lys Lys Ile Gly 
385                 390                 395                 400 


Ser Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile 
                405                 410                 415     


Trp Gln Val Leu His Asn Val Asp Ile Lys Asn Lys Lys Asp Lys Thr 
            420                 425                 430         


Lys Arg Pro Leu Asp Asp Ser Glu Lys Glu Tyr Leu Phe Asn Glu Leu 
        435                 440                 445             


Asn Ile Lys Gly Asn Leu Lys Ala Ala Lys Val Ile Gln Leu Leu Thr 
    450                 455                 460                 


Leu Asn Lys Asn Glu Thr Glu Leu Asn Tyr Thr Gln Leu Glu Gly Asn 
465                 470                 475                 480 


Arg Thr Gln Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Leu Asp Thr 
                485                 490                 495     


Glu Gly Tyr Asp Val Asn Asn Leu Leu Gly Ile Lys Ser Asn Lys Asp 
            500                 505                 510         


Glu Val Phe Leu Asp Asp Leu Asp Val Asn Ala Leu Glu Ile Lys Thr 
        515                 520                 525             


Met Ile Lys Gln Ile Phe Asn Ala Leu Asn Ile Asn Thr Gly Ile Leu 
    530                 535                 540                 


Asp Phe Asp Ala Glu Leu Asp Gly Lys Ala Phe Glu Gln Gln Ala Ser 
545                 550                 555                 560 


Tyr His Leu Trp His Leu Leu Tyr Ser Tyr Glu Glu Asp Gly Ser Lys 
                565                 570                 575     


Ser Gly Asn Glu His Leu Tyr Lys Leu Leu Glu Asn Lys Phe Gly Phe 
            580                 585                 590         


Lys Pro Glu His Ala Arg Ile Leu Ala Gly Ile Thr Leu Ser Asp Asp 
        595                 600                 605             


Tyr Gly Asn Leu Ser Ala Lys Ala Met Arg Lys Ile Tyr Pro Gln Ile 
    610                 615                 620                 


Lys Glu Asn Asn Phe Ser Thr Ala Cys Glu Leu Val Gly Tyr Arg His 
625                 630                 635                 640 


Ser Lys Asn Ser Leu Thr Lys Glu Glu Asn Gln Glu Arg Pro Leu Lys 
                645                 650                 655     


Asn Lys Leu Glu Leu Leu Lys Lys Asn Ser Leu Arg Gln Pro Val Val 
            660                 665                 670         


Glu Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Glu Leu Ile Asp 
        675                 680                 685             


Lys Asn Ser Glu Lys Asp Glu Gln Gly Ser Ile Ile Glu Tyr Phe Arg 
    690                 695                 700                 


Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Thr 
705                 710                 715                 720 


Asp Glu Arg Arg Asp Leu Asp Ala Ala Ile Arg Glu Gly Asn Leu Arg 
                725                 730                 735     


Asn Glu Lys Ile Tyr Lys Leu Leu Gln Ala Gln Phe Gly Ile Lys Asn 
            740                 745                 750         


Pro Thr Arg Asn Asp Ile Ile Arg Tyr Arg Leu Tyr Glu Glu Leu Lys 
        755                 760                 765             


Asn Asn Gly Tyr Lys Asp Leu Tyr Thr Asn Ser Tyr Ile Pro Gln Glu 
    770                 775                 780                 


Lys Leu Phe Gly Lys Glu Ile Asp Ile Asp His Ile Ile Pro Gln Ser 
785                 790                 795                 800 


Lys Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Thr Tyr Asn Thr 
                805                 810                 815     


Thr Asn Lys Asp Lys Gly Asn Gln Thr Gly Phe Asp Tyr Ile Glu Ser 
            820                 825                 830         


Lys Tyr Gly Ile Asp Gly Ile Glu Ala Tyr Thr Ala Arg Ile Glu Asn 
        835                 840                 845             


Leu Phe Glu Ser Gly Lys Lys Asn Lys Glu Glu Gly Val Ser Lys Ala 
    850                 855                 860                 


Lys Tyr Gln Lys Leu Leu Lys Lys Glu Ser Glu Ile Gly Asp Gly Phe 
865                 870                 875                 880 


Ile Glu Arg Asp Leu Arg Glu Ser Gln Tyr Ile Ala Lys Lys Ala Lys 
                885                 890                 895     


Glu Leu Leu Leu Gln Ile Thr Arg Ser Val Val Ala Thr Ser Gly Ser 
            900                 905                 910         


Ile Thr Ala Arg Leu Arg Glu Asp Trp Asp Leu Val Asn Val Met Lys 
        915                 920                 925             


Glu Leu Asn Leu Pro Lys Tyr Arg Ala Leu Gly Leu Thr Glu Met Glu 
    930                 935                 940                 


Glu Arg Lys Tyr Gly Gln Lys Val Glu Val Ile Lys Asp Trp Thr Lys 
945                 950                 955                 960 


Arg Asp Asp His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe 
                965                 970                 975     


Thr Lys His Asn His Ile Gln Tyr Leu Asn His Leu Asn Ala Arg Lys 
            980                 985                 990         


Asp Glu Gln His Lys Glu His Lys  Ile Ile Asn Ala Ile  Glu Asn Lys 
        995                 1000                 1005             


Glu Thr  Ile Leu Ile Thr Asp  Lys Ser Gly Asn Arg  Lys Arg Val 
    1010                 1015                 1020             


Phe Lys  Ala Pro Met Pro Asn  Phe Arg Gln Thr Ala  Lys Gln Tyr 
    1025                 1030                 1035             


Leu Glu  Glu Val Leu Ile Ser  His Lys Ala Lys Asn  Lys Val Val 
    1040                 1045                 1050             


Thr Lys  Asn Lys Asn Lys Ile  Leu Gly Lys Glu Lys  Ile Gln Glu 
    1055                 1060                 1065             


Thr Leu  Thr Pro Arg Gly Gln  Leu His Asp Gln Thr  Ile Tyr Gly 
    1070                 1075                 1080             


Phe Asn  Trp Trp Ser Arg Ile  Thr Leu Ser Lys Lys  Val Ser Arg 
    1085                 1090                 1095             


Asn Glu  Ile Glu Lys Ile Ile  Asp Asn Asn Thr Lys  Phe Phe Leu 
    1100                 1105                 1110             


Gln Glu  Phe Ile Gln Leu Lys  Gly Ser Ile Ala Lys  Ala Phe Ser 
    1115                 1120                 1125             


Asp Ala  Glu Leu Asn Lys Leu  Met Tyr Lys Gly Lys  Ile Leu Lys 
    1130                 1135                 1140             


Glu Ile  Glu Val Ala Ile Pro  Cys Tyr Thr Gln Arg  Ile Asp Ile 
    1145                 1150                 1155             


Ser Lys  Val Phe Thr Asp Ser  Gln Lys Ser Asn Ser  Ala Lys Glu 
    1160                 1165                 1170             


Lys Asn  Ile Lys Asn Ile Leu  Asp Leu Lys Val Arg  Asn Ile Leu 
    1175                 1180                 1185             


Gln Asn  Arg Leu Ser Glu Phe  Lys Gly Asp Phe Lys  Lys Ala Phe 
    1190                 1195                 1200             


Ser Asp  Leu Asp Lys Asn Pro  Ile Trp Phe Asn Lys  Glu Lys Gly 
    1205                 1210                 1215             


Ile Ser  Ile Lys Arg Val Ile  Ile Ser Gly Val Lys  Asn Val Glu 
    1220                 1225                 1230             


Pro Leu  His Tyr Lys Lys Asp  His Leu Gly Asn Asp  Ile Leu Asp 
    1235                 1240                 1245             


Gly Lys  Glu Asn Arg Ile Pro  Val Asp Phe Ile Ser  Thr Gly Asn 
    1250                 1255                 1260             


Asn His  His Val Ala Ile Tyr  Gln Asp Glu Lys Gly  Asn Leu Gln 
    1265                 1270                 1275             


Glu Lys  Val Val Ser Leu Phe  Asp Ala Val Lys Leu  Val Asn Ala 
    1280                 1285                 1290             


Asp Glu  Pro Val Ile Asp Lys  Thr Tyr Asn Gln Gly  Leu Gly Trp 
    1295                 1300                 1305             


Gln Phe  Leu Phe Thr Met Lys  Gln Asn Glu Met Phe  Val Phe Pro 
    1310                 1315                 1320             


Asn Asp  Asn Thr Gly Phe Asp  Pro Lys Glu Ile Asp  Leu Leu Asp 
    1325                 1330                 1335             


Pro Ser  Asn Lys Lys Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln 
    1340                 1345                 1350             


Ser Ile  Ser Ile Val Gln Tyr  Gly Asn Asn Ile Val  Arg Asp Phe 
    1355                 1360                 1365             


Lys Phe  Arg Asn His Leu Glu  Ser Val Leu Asn Asp  Asp Lys Val 
    1370                 1375                 1380             


Leu His  Gly Ile Thr Phe Gln  Gln Ile Lys Ser Leu  Gly Pro Leu 
    1385                 1390                 1395             


Leu Asn  Ile Val Lys Val Arg  Ile Asn His Leu Gly  Asp Ile Val 
    1400                 1405                 1410             


Ser Val  Gly Glu Tyr 
    1415             


<210>  1205
<211>  1419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1205

Met Ala Lys Pro Gln Asn Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Thr Val Thr Asp Asp Gln Asn Asn Ile Leu Arg Ile 
            20                  25                  30          


Lys Ser Lys Lys Ala Ile Gly Ala Arg Leu Phe Glu Glu Gly Lys Val 
        35                  40                  45              


Ala Ala Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg 
    50                  55                  60                  


Arg Lys Trp Arg Ile Lys Leu Leu Glu Glu Leu Phe Asp Glu Glu Ile 
65                  70                  75                  80  


Ser Lys Val Asp Pro Asn Phe Phe Ala Arg Ser His Glu Ser Trp Ile 
                85                  90                  95      


Ser Pro Lys Asp Lys Arg Glu Arg Tyr Ser Ala Ile Val Phe Pro Thr 
            100                 105                 110         


Pro Glu Glu Asp Lys Lys Phe His Asn Glu Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Asp Arg Leu Met Lys Asp Asp Gln Lys His Asp Ile Arg Glu 
    130                 135                 140                 


Ile Tyr Val Ala Ile His His Ile Met Lys Tyr Arg Gly Asn Phe Leu 
145                 150                 155                 160 


His Asp Glu Ser Ile Glu Thr Tyr Arg Ser Gly Met Ser Ser Leu Gly 
                165                 170                 175     


Gly Gly Asp Glu Arg Thr Ile Leu Asn Thr Lys Ile Leu Asp Asp Leu 
            180                 185                 190         


Asn Asp Ala Phe Ala Asp Asn Leu Ala Thr Gln Gly Ile Ser Val Asn 
        195                 200                 205             


Ser Ala Ser Ala Asp Gln Ile Asn Glu Ile Val Thr Ser Gly His Leu 
    210                 215                 220                 


Asn Val Asp Ser Gln Lys Ala Ile Ala Lys Leu Leu Leu Pro Thr Thr 
225                 230                 235                 240 


Leu Ser Ser Asp Asp Lys Lys Gln Asp Lys Lys Ile Lys Lys Leu Ala 
                245                 250                 255     


Thr Asp Ala Ala Lys Asn Val Ala Ser Ala Trp Leu Gly Tyr Lys Ala 
            260                 265                 270         


Asn Phe Ala Ala Ile Leu Asn Ile Gln Glu Val Asp Lys Asp Lys Lys 
        275                 280                 285             


Lys Ile Tyr Ser Phe Ala Leu Gln Gly Ala Asp Glu Glu Asp Lys Val 
    290                 295                 300                 


Glu Thr Leu Glu Ser Val Leu Ser Gln Asp Gln Ile Glu Ile Val Asp 
305                 310                 315                 320 


Arg Ile Ile Arg Ile Arg His Ala Ile Val Leu Gly Glu Ile Ile Pro 
                325                 330                 335     


Val Gly Met Thr Leu Ser Glu Ala Met Ile Glu Lys Tyr Asp Gln His 
            340                 345                 350         


Ala Lys Asp Leu Lys Asn Leu Lys Thr Val Ile Arg Asn Leu Lys Asp 
        355                 360                 365             


Arg Lys Asn Ala Val Lys Leu Gln Ala Ile Tyr Asp Leu Tyr Val Asn 
    370                 375                 380                 


Asn Arg His Ala Asp Thr Ala Lys Ala Met Lys Leu Ala Gly Val Lys 
385                 390                 395                 400 


Lys Arg Thr Glu Leu Leu Glu Pro Glu Glu Phe Asn Lys Ser Ile Glu 
                405                 410                 415     


Lys Leu Leu Asp Asp Ser Pro Val Ser Ala Glu Ile Lys Lys Arg Ile 
            420                 425                 430         


Ala Glu Lys Asp Phe Leu Pro Leu Gln Arg Ser Asn Ser Asn Gly Val 
        435                 440                 445             


Ile Pro Asn Gln Leu His Gln Ile Glu Leu Asp Glu Ile Ile Lys Lys 
    450                 455                 460                 


Gln Ser Lys Tyr Tyr Pro Phe Leu Gly Glu Thr Asn Glu His Ala Ser 
465                 470                 475                 480 


Ala Asp Val Ala Arg Lys Ala Pro Thr Lys Leu Asp Gln Leu Leu Thr 
                485                 490                 495     


Phe Arg Val Pro Tyr Tyr Val Gly Pro Leu Ile Thr Lys Glu Glu Gln 
            500                 505                 510         


Glu Glu Gln Asp Gly His Ser Phe Ala Trp Met Ile Arg Lys Asn Pro 
        515                 520                 525             


Lys Asp His Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu Lys Val Asp 
    530                 535                 540                 


Arg Met Ala Ser Ala Thr Gln Phe Ile Lys Arg Met Thr Thr Lys Asp 
545                 550                 555                 560 


Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Ser Ser Ser Leu Lys Tyr 
                565                 570                 575     


Gln Leu Phe Thr Val Leu Asn Glu Leu Asn Asn Leu Arg Ile Asn His 
            580                 585                 590         


Lys Lys Leu Thr Arg Glu Glu Lys Glu Gln Val Ile Glu Asp Leu Phe 
        595                 600                 605             


Lys Lys Tyr Lys Thr Val Lys Ala Asp Lys Phe Lys Asn Tyr Trp Gln 
    610                 615                 620                 


Lys Gly His Ala Gly Leu Asp Val Lys Ile Gln Gly Met Ala Asp Pro 
625                 630                 635                 640 


Glu Lys Phe Asn Ser Thr Met Ser Thr Tyr Ile Asp Leu Gln Lys Ile 
                645                 650                 655     


Phe Gly Asp Gln Leu Asp Asp Ala Gln Arg Gln Asn Asp Phe Glu Lys 
            660                 665                 670         


Ile Ile Glu Tyr Ser Thr Ile Phe Glu Asp Arg Lys Ile Leu Gly Glu 
        675                 680                 685             


Lys Leu Arg Ala Asp Phe Val Trp Leu Ser Glu Asp Gln Ile Lys Arg 
    690                 695                 700                 


Leu Cys Arg Leu Arg Leu Gln Gly Trp Gly Arg Leu Ser Leu Lys Leu 
705                 710                 715                 720 


Leu Thr Arg Leu Thr Asp Ala Asp Gly Gln Asn Val Leu Glu Arg Leu 
                725                 730                 735     


Leu Asn Ser Asn Asp Asn Phe Met Gln Ile Val Lys Asp Pro Val Ile 
            740                 745                 750         


Lys Ala Lys Ile Glu Gln Lys Asn Gln Lys Leu Leu Arg Ala Asp Trp 
        755                 760                 765             


Ser Glu Asn Ala Thr Glu Ser Ile Leu Asp Glu Ala Tyr Thr Ser Pro 
    770                 775                 780                 


Gln Asn Lys Lys Ala Ile Arg Gln Val Met Arg Val Val His Asp Ile 
785                 790                 795                 800 


Val Lys Ala Cys Asp Gly Asn Leu Pro Ala Lys Phe Ala Ile Glu Phe 
                805                 810                 815     


Ala Arg Ser Gln Asp Asp Asp Pro Lys Arg Thr Val Ala Arg Ala Asp 
            820                 825                 830         


Arg Leu Gln Lys Ile Tyr Ala Gln Ile Ser Asp Glu Val Val Ser Gln 
        835                 840                 845             


Gly Val Lys Asp Gln Leu Ser Gly Met Lys Ser Leu Lys Asp Arg Tyr 
    850                 855                 860                 


Tyr Leu Tyr Phe Met Gln Gly Gly Arg Asp Ala Tyr Thr Gly Lys Pro 
865                 870                 875                 880 


Leu Asn Ile Asp Arg Leu Ser Asp Tyr Asp Ile Asp His Ile Met Pro 
                885                 890                 895     


Gln Ser Phe Ile Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Thr 
            900                 905                 910         


Arg Ala Val Asn Asn Gln Lys Ser Asp Asn Val Pro Val Leu Leu Tyr 
        915                 920                 925             


Gly Asn His Leu Val Ala Asp Leu Gly Ile Thr Ile Arg Glu Met Trp 
    930                 935                 940                 


Asp Arg Trp Gln Lys Ser Gly Met Ile Ser Lys Arg Lys Leu Ala Asn 
945                 950                 955                 960 


Leu Leu Thr Asp Pro Ala Thr Leu Ser Glu Tyr Arg Ala Glu Gly Phe 
                965                 970                 975     


Ile Asn Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Thr Ala 
            980                 985                 990         


Thr Ile Leu Gln Ser Glu Phe Pro  Asp Ser Lys Ile Ile  Glu Val Pro 
        995                 1000                 1005             


Ala Lys  Tyr Asn Ser Ile Leu  Arg Lys Gln Phe Asp  Leu Tyr Lys 
    1010                 1015                 1020             


Ser Arg  Glu Val Asn Asp Tyr  His His Ala Ile Asp  Ala Tyr Leu 
    1025                 1030                 1035             


Ser Thr  Ile Val Gly Asn Tyr  Leu Tyr Gln Val Tyr  Pro Asn Leu 
    1040                 1045                 1050             


Arg Arg  Leu Phe Val Tyr Gly  Glu Phe Lys Lys Phe  Ser His Gly 
    1055                 1060                 1065             


Glu Glu  Glu Ser Ser His Gln  Ala Val Arg Arg Val  Lys Ser Met 
    1070                 1075                 1080             


Asn Phe  Leu Asp Asp Leu Leu  Arg Gly Lys His Gly  Asp Asp Ile 
    1085                 1090                 1095             


Tyr Cys  Arg Val Thr Gly Glu  Val Ile Phe Ser Arg  Lys Asp Ile 
    1100                 1105                 1110             


Ile Ser  Lys Leu Lys Gln Ala  Tyr Ser Phe Lys Gln  Met Leu Val 
    1115                 1120                 1125             


Thr Gln  Glu Val Phe Thr Lys  Lys Ser Ala Leu Phe  Asp Gln Thr 
    1130                 1135                 1140             


Val Tyr  Pro Ser Pro Ala Arg  Asp Ser Lys Thr Arg  Ser Gly Leu 
    1145                 1150                 1155             


Ile Pro  Arg Lys Gln Gly Met  Asp Thr Glu Ile Tyr  Gly Gly Tyr 
    1160                 1165                 1170             


Ser Gly  Asn Lys Asp Ala Tyr  Phe Val Leu Ala Gln  Val Val Lys 
    1175                 1180                 1185             


Ser Lys  Gly His Thr Leu Gln  Val Val Gly Val Pro  Ile Arg Ala 
    1190                 1195                 1200             


Leu Arg  Ala Leu Ala Asn Cys  Ser Asn Tyr Ser Glu  Gln Leu Leu 
    1205                 1210                 1215             


Lys Ile  Val Lys Ala Gln Val  Leu Phe Asn Lys Lys  Thr Gly Lys 
    1220                 1225                 1230             


Ala Lys  Lys Gly Ile Ser Asp  Val Arg Ile Leu Leu  Asp Lys Ile 
    1235                 1240                 1245             


Pro Tyr  Arg Gln Pro Val Leu  Asp Gly Ser Ala Phe  Tyr Met Leu 
    1250                 1255                 1260             


Gly Ser  Ser Thr Tyr Lys Tyr  Asn Leu Lys Gln Ile  Ser Leu Ser 
    1265                 1270                 1275             


Gln Gln  Ser Met Lys Tyr Ile  Leu Asp Tyr Ile Asp  Asp Pro Asn 
    1280                 1285                 1290             


Phe Asn  Lys His Glu Met Thr  Lys Ile Asp Gln Gln  Asn Glu Lys 
    1295                 1300                 1305             


Glu Cys  Leu Leu Ser Val Tyr  Asp Glu Ile Leu Asp  Lys Met Asp 
    1310                 1315                 1320             


Lys Tyr  Leu Ser Leu Tyr Asp  Ile Asn Gly Phe Arg  Lys Lys Leu 
    1325                 1330                 1335             


His Val  Gly Arg Asp Lys Phe  Ile Lys Leu Glu Val  Glu Lys Gln 
    1340                 1345                 1350             


Ala Glu  Ala Ile Lys Asn Val  Leu Arg Gly Phe His  Ala Asn Ser 
    1355                 1360                 1365             


Asp Met  Thr Lys Leu Ser Asp  Leu Gly Leu Ser Thr  Ser Leu Gly 
    1370                 1375                 1380             


Phe Met  Gln Ala Ser Asp Gly  Ile Lys Leu Ser Asp  Asn Ala Val 
    1385                 1390                 1395             


Phe Ile  Tyr Gln Ser Pro Thr  Gly Leu Phe Glu Arg  Arg Val Lys 
    1400                 1405                 1410             


Val Ser  Asp Leu Leu Lys 
    1415                 


<210>  1206
<211>  1419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1206

Met Met Ile Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Trp Ala Leu Ile Lys Gln Asn Phe Glu Asn Lys Tyr Gly Glu Ile 
            20                  25                  30          


Leu Gly Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly 
        35                  40                  45              


Glu Phe Gly Lys Gly Asn Ser Val Ser Gln Thr Ala Ala Arg Thr Gly 
    50                  55                  60                  


Tyr Arg Gly Val Arg Arg Leu Arg Glu Arg Phe Leu Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Arg Val Leu Asn Val Leu Asn Phe Leu Pro Glu His Tyr 
                85                  90                  95      


Ala Ser Gln Ile Asp Phe Glu Lys Arg Phe Gly Lys Phe Lys Tyr Glu 
            100                 105                 110         


Thr Glu Pro Lys Leu Ala Tyr Asn Asn Asp Gly Phe Phe Phe Lys Asn 
        115                 120                 125             


Ser Phe Asp Glu Met Leu Gly Asp Phe Lys Asp His Gln Phe His Leu 
    130                 135                 140                 


Leu Glu Asn Gly Arg Lys Val Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu 
145                 150                 155                 160 


Arg Lys Lys Ala Leu Ser Gln Lys Ile Glu Lys Glu Glu Leu Ala Trp 
                165                 170                 175     


Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly 
            180                 185                 190         


Glu Asp Phe Glu Glu Glu Lys Asp Arg Val Phe Val Arg Leu Lys Val 
        195                 200                 205             


Asp Arg Val Val Asp Ser Gly Glu Asn Ile Lys Gly Lys Ile Leu Tyr 
    210                 215                 220                 


Asp Val Tyr Phe Glu Asn Gly Trp Lys Tyr Glu Lys Gln Ile Val Lys 
225                 230                 235                 240 


Thr Glu Asp Trp Ile Gly Arg Thr Lys Glu Phe Ile Val Thr Glu Ser 
                245                 250                 255     


Ile Leu Lys Asn Gly Asp Thr Lys Arg Thr Phe Lys Ala Val Asp Ser 
            260                 265                 270         


Glu Lys Asp Trp Ile Ala Ile Lys Thr Lys Thr Glu Gln Glu Ile Asp 
        275                 280                 285             


Lys Ser His Lys Thr Val Gly Ala Tyr Ile Tyr Glu Ala Leu Leu Gln 
    290                 295                 300                 


Asn Pro Lys Gln Lys Ile Lys Gly Lys Leu Val Arg Thr Ile Glu Arg 
305                 310                 315                 320 


Lys Phe Tyr Arg Glu Glu Leu Lys Lys Ile Leu Glu Arg Gln Lys Glu 
                325                 330                 335     


Phe His Pro Glu Leu Gln Ser Asp Asp Leu Tyr Asn Asp Cys Ile Arg 
            340                 345                 350         


Glu Leu Tyr Arg Asn Asn Glu Ala His Gln Ile Thr Leu Ser Lys Lys 
        355                 360                 365             


Asp Phe Val His Leu Leu Leu Asn Asp Leu Ile Phe Tyr Gln Arg Pro 
    370                 375                 380                 


Leu Arg Ser Gln Lys Ser Ser Val Ser Asn Cys Thr Leu Glu Phe Arg 
385                 390                 395                 400 


Lys Tyr Lys Asp Glu Asn Gly Val Glu His Ala Gln Tyr Leu Lys Thr 
                405                 410                 415     


Ile Pro Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Val Trp Gln Trp 
            420                 425                 430         


Met Phe Asn Leu Asn Ile Tyr Arg Lys Asp Asp Asp Thr Asn Val Thr 
        435                 440                 445             


Lys Glu Phe Leu Ser Ser Thr Glu Asp Tyr Glu Thr Leu Phe Glu Phe 
    450                 455                 460                 


Leu Asn Asn Arg Lys Glu Val Asp Gln Lys Ala Leu Leu Arg His Phe 
465                 470                 475                 480 


Lys Leu Asn Glu Lys Thr His Arg Trp Asn Tyr Val Glu Asp Lys Lys 
                485                 490                 495     


Tyr Pro Cys Asn Glu Thr Lys Thr Met Ile Ser Thr Arg Leu Glu Lys 
            500                 505                 510         


Val Glu Asn Tyr Pro Ser Asp Phe Leu Thr Arg Glu Met Glu Gln Asn 
        515                 520                 525             


Ile Trp His Ile Ile Tyr Ser Val Asn Asp Lys Ile Glu Tyr Glu Lys 
    530                 535                 540                 


Ala Leu Lys Ser Phe Ala His Lys Asn Asn Leu Asp Glu Asn Ser Phe 
545                 550                 555                 560 


Phe Glu Ala Phe Lys Lys Phe Pro Pro Phe Lys Ser Glu Tyr Gly Ser 
                565                 570                 575     


Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu Leu Arg Leu Gly 
            580                 585                 590         


Lys Tyr Trp Asn Trp Glu Ser Ile Asp Arg Lys Ser Gln Gly Arg Ile 
        595                 600                 605             


Glu Lys Ile Ile Thr Gly Glu Phe Asp Gly Asn Ile Lys Asp Lys Val 
    610                 615                 620                 


Arg Glu Lys Ala Ile His Leu Thr Ala Glu Asn Asp Phe Gln Gly Leu 
625                 630                 635                 640 


Gln Leu Trp Leu Ala Gln Tyr Ile Val Tyr Gly Arg His Ser Glu Ala 
                645                 650                 655     


Ser Met Ile Gly Lys Trp Asn Ser Ala Asp Asp Leu Glu Glu Phe Leu 
            660                 665                 670         


Lys Gly Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val 
        675                 680                 685             


Ile Thr Glu Thr Leu Arg Val Val Lys Asp Ile Trp Leu Lys Tyr Gly 
    690                 695                 700                 


Asn Gly Ala Lys Asp Phe Phe Ser Glu Ile His Ile Glu Leu Gly Arg 
705                 710                 715                 720 


Glu Met Lys Gln Thr Lys Asp Glu Arg Val Asp Ala Thr Lys Arg Ile 
                725                 730                 735     


Thr Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala Leu Leu Ala Glu 
            740                 745                 750         


Met Met Asn Asp Asn Ser Val Glu Asn Ile Arg Pro Tyr Ser Pro Met 
        755                 760                 765             


Gln Gln Glu Ile Leu Lys Ile Tyr Glu Asp Gly Ile Leu Lys Ser Asp 
    770                 775                 780                 


Ile Glu Ile Asp Asp Asp Ile Leu Lys Ile Ser Lys Thr Ala Gln Pro 
785                 790                 795                 800 


Ser Ser Ser Asp Leu Lys Arg Tyr Lys Leu Trp Leu Glu Gln Arg Tyr 
                805                 810                 815     


Lys Ser Pro Tyr Thr Gly Gln Ile Ile Pro Leu Asn Lys Leu Phe Thr 
            820                 825                 830         


Ser Glu Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Phe Asp 
        835                 840                 845             


Asp Ser Phe Ser Asn Lys Ile Ile Cys Glu Ser Ala Val Asn Lys Leu 
    850                 855                 860                 


Lys Asp Asn Tyr Ile Gly Leu Gly Phe Ile Lys Gln Phe Gly Gly Met 
865                 870                 875                 880 


Ile Val Glu Cys Gly Phe Gly Lys Ser Val Glu Ile Phe Lys Ala Glu 
                885                 890                 895     


Glu Tyr Glu Asp Phe Val Lys Lys His Tyr Ala Asn Asn Arg Ser Lys 
            900                 905                 910         


Arg Asn Lys Leu Leu Leu Glu Glu Ile Pro Glu Lys Met Ile Asp Arg 
        915                 920                 925             


Gln Leu Asn Asp Thr Arg His Ile Ser Lys Tyr Ile Ser Gly Val Leu 
    930                 935                 940                 


Ser Asn Ile Val Arg Gln Glu Asp Gly Thr Asp Glu Gly Val Asn Ser 
945                 950                 955                 960 


Lys Asn Ile Val Pro Gly Asn Gly Lys Ile Thr Thr Gln Leu Lys Gln 
                965                 970                 975     


Asp Trp Gly Leu Asn Asp Val Trp Asn Glu Leu Ile Leu Pro Arg Phe 
            980                 985                 990         


Glu Arg Met Asn Gln Leu Thr Asn  Ser Thr Asp Phe Thr  Ser Trp Asn 
        995                 1000                 1005             


Lys Asn  His Gln Lys Phe Leu  Pro Thr Val Pro Ile  Glu Phe Ser 
    1010                 1015                 1020             


Lys Gly  Phe Ser Lys Lys Arg  Ile Asp His Arg His  His Ala Met 
    1025                 1030                 1035             


Asp Ala  Leu Val Ile Ala Cys  Ala Thr Lys Asp His  Ile Asn Leu 
    1040                 1045                 1050             


Leu Asn  Asn Gln Ser Ala Lys  Ser Asp Thr Lys Arg  Tyr Asp Leu 
    1055                 1060                 1065             


Lys Arg  Lys Leu Met Lys Phe  Glu Arg Val Val Tyr  Ser His Ser 
    1070                 1075                 1080             


Lys Thr  Gly Glu Lys Ile Glu  Lys Glu Val Pro Lys  Gln Phe Leu 
    1085                 1090                 1095             


Lys Pro  Trp Asp Asn Phe Thr  Val Asp Ala Lys Asn  Lys Met Glu 
    1100                 1105                 1110             


Thr Ile  Ile Val Ser Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys 
    1115                 1120                 1125             


Ala Thr  Asn His Tyr Glu Lys  Trp Ile Glu Lys Asp  Gly Ile Lys 
    1130                 1135                 1140             


Arg Lys  Glu Leu Ile Lys Gln  Glu Gly Thr Asn Trp  Ala Ile Arg 
    1145                 1150                 1155             


Lys Pro  Met His Lys Glu Thr  Val Ser Gly Lys Val  Asp Leu Pro 
    1160                 1165                 1170             


Gly Ile  Lys Ile Pro Lys Gly  Lys Ile Leu Thr Ala  Thr Arg Lys 
    1175                 1180                 1185             


Ser Leu  Asp Thr Ser Phe Asp  Leu Lys Thr Ile Asn  Ser Ile Thr 
    1190                 1195                 1200             


Asp Thr  Gly Ile Gln Lys Ile  Leu Lys Asn Tyr Leu  Glu Phe Lys 
    1205                 1210                 1215             


Gly Ser  Ser Glu Leu Ala Phe  Ser Pro Glu Gly Ile  Glu Asp Met 
    1220                 1225                 1230             


Asn Lys  Asn Ile Glu Lys Phe  Asn Asp Gly Lys Pro  His Gln Ser 
    1235                 1240                 1245             


Ile Asn  Lys Val Arg Ile Phe  Glu Leu Gly Ser Lys  Phe Ser Val 
    1250                 1255                 1260             


Gly Gln  Thr Gly Asn Lys Lys  Asp Lys Tyr Val Glu  Ala Ala Lys 
    1265                 1270                 1275             


Gly Thr  Asn Leu Phe Phe Ala  Val Tyr Glu Asp Lys  Asn Gly Lys 
    1280                 1285                 1290             


Arg Ser  Tyr Glu Thr Ile Pro  Leu Asn Glu Val Ile  Glu Arg Gln 
    1295                 1300                 1305             


Lys Gln  Gly Leu Asp Ser Val  Ser Phe Lys Asn Lys  Glu Asp Phe 
    1310                 1315                 1320             


Leu Leu  Cys Pro Asn Asp Leu  Val Tyr Val Pro Ser  Glu Asp Glu 
    1325                 1330                 1335             


Leu Glu  Asn Ile Tyr Asn Ile  Asp Phe Lys Asn Leu  Thr Thr Glu 
    1340                 1345                 1350             


Lys Thr  Gly Asn Ile Tyr Lys  Val Val Ser Phe Ser  Gly Asn Gln 
    1355                 1360                 1365             


Ile Phe  Phe Ile Arg Gln Asp  Ile Ala Ile Ser Ile  Val Asn Lys 
    1370                 1375                 1380             


Ala Glu  Phe Ser Thr Leu Asn  Lys Met Glu Arg Ala  Ile Asp Gly 
    1385                 1390                 1395             


Ser Met  Ile Lys Glu Asn Cys  Ile Lys Leu Lys Ile  Asp Arg Leu 
    1400                 1405                 1410             


Gly Asn  Ile Ala Lys Ile 
    1415                 


<210>  1207
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1207

Met Ala Lys Pro Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Val Val Thr Asp Asp Gln Asn Asn Ile Leu Arg Ile 
            20                  25                  30          


Lys Gly Lys Lys Ala Ile Gly Ala Arg Leu Phe Thr Glu Gly Lys Val 
        35                  40                  45              


Ala Ala Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg 
    50                  55                  60                  


Arg Arg Trp Arg Ile Lys Met Leu Glu Glu Leu Phe Asp Glu Glu Ile 
65                  70                  75                  80  


Ala Lys Val Asp Pro Ser Phe Phe Ala Arg Leu His Glu Ser Trp Ile 
                85                  90                  95      


Ser Pro Lys Asp Lys Arg Lys Arg Tyr Ser Ala Ile Val Phe Pro Ser 
            100                 105                 110         


Pro Glu Glu Asp Lys Lys Phe His Glu Ser Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Asp Lys Leu Met Lys Asp Asp Gln Lys His Asp Ile Arg Glu 
    130                 135                 140                 


Ile Tyr Ile Ala Val His Gln Met Ile Lys Ala Arg Gly Asn Phe Leu 
145                 150                 155                 160 


His Asp Glu Ser Val Glu Thr Tyr Arg Ser Gly Met Ser Ser Leu Gly 
                165                 170                 175     


Gly Arg Ser Glu Arg Thr Ile Leu Ser Val Gln Thr Leu Glu Glu Leu 
            180                 185                 190         


Asn Asp Leu Phe Ala Glu Asn Glu Gly Thr Glu Glu Val Glu Leu Asn 
        195                 200                 205             


Val Ala Ser Ala Glu Gln Ile Asn Asp Ile Leu Thr Gly Gly His Leu 
    210                 215                 220                 


Asn Ala Asp Ser Gln Lys Glu Ile Ser Asn Leu Leu Leu Pro Ser Ser 
225                 230                 235                 240 


Phe Pro Ser Phe Asp Asp Lys Ala Lys Glu Lys Gln Val Lys Lys Leu 
                245                 250                 255     


Ile Asn Asn Val Ala Thr Asn Ile Ser Lys Ala Trp Leu Gly Tyr Lys 
            260                 265                 270         


Ala Asp Phe Ser Thr Ile Leu Asn Leu Ala Lys Val Asp Lys Asp Gln 
        275                 280                 285             


Lys Lys Ile Phe Ala Phe Ala Leu Gln Gly Glu Asp Glu Glu Asp Lys 
    290                 295                 300                 


Val Gln Glu Leu Glu Ser Leu Leu Glu Gln Ser Gln Thr Asp Ile Val 
305                 310                 315                 320 


Asp Arg Leu Ile Glu Ile Arg His Ala Ile Val Leu Ser Glu Ile Val 
                325                 330                 335     


Pro Val Gly Met Thr Leu Ser Glu Ala Met Ile Asp Lys Tyr Asp Gln 
            340                 345                 350         


His Lys Glu Asp Leu Ile Thr Leu Lys Ala Val Ile Arg Asn Thr Lys 
        355                 360                 365             


Asp Lys Lys Lys Ala Ala Lys Leu Gln Ala Ile Tyr Asp Leu Tyr Val 
    370                 375                 380                 


Lys Asn Arg His Ala Asp Leu Ala Lys Ala Met Lys Leu Thr Gly Ile 
385                 390                 395                 400 


Lys Lys Arg Ser Glu Leu Leu Asp Pro Glu Glu Leu Lys Lys Gly Ile 
                405                 410                 415     


Ser Ser Leu Leu Asp Asp Ser Pro Glu Ala Val Glu Ile Lys Gln Arg 
            420                 425                 430         


Leu Glu Glu Lys Thr Phe Leu Pro Leu Gln Arg Ser Asn Asn Asn Gly 
        435                 440                 445             


Val Ile Pro Asn Gln Leu His Gln Val Glu Leu Asp Glu Ile Ile Lys 
    450                 455                 460                 


Lys Gln Ser Lys Tyr Tyr Pro Phe Leu Ala Glu Lys Asn Pro Asp Glu 
465                 470                 475                 480 


Ser Glu Glu Ala Gln Lys Lys Ala Pro Thr Lys Leu Asp Ala Leu Leu 
                485                 490                 495     


Thr Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Lys Glu Glu 
            500                 505                 510         


Gln Glu Ala Gln Asn Gly His Ser Phe Ala Trp Met Val Arg Arg Asp 
        515                 520                 525             


Pro Gln Asp His Glu Ala Ile Thr Pro Trp Asn Phe Glu Lys Lys Val 
    530                 535                 540                 


Asp Lys Met Ala Ser Ala Thr Gln Phe Ile Lys Arg Met Thr Thr Lys 
545                 550                 555                 560 


Asp Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Ala Ser Ser Leu Lys 
                565                 570                 575     


Tyr Gln Leu Phe Thr Val Leu Asn Glu Leu Asn Asn Leu Arg Val Asn 
            580                 585                 590         


Gly Lys Lys Leu Thr Ser Asp Glu Lys Glu Gln Val Ile Glu Gly Leu 
        595                 600                 605             


Phe Lys Lys Gln Lys Thr Val Lys Ala Asp Lys Phe Val Lys Tyr Trp 
    610                 615                 620                 


Gln Ala Lys His Ile Gly Ala Asp Ile Lys Val Lys Gly Leu Ser Asp 
625                 630                 635                 640 


Pro Ser Lys Phe Asn Ser Thr Met Ser Thr Tyr Ile Asp Phe Lys Lys 
                645                 650                 655     


Ile Phe Gly Asp Gln Leu Asn Asp Val Asn Arg Gln Asn Asp Phe Glu 
            660                 665                 670         


Lys Ile Ile Glu Tyr Ser Thr Ile Phe Glu Asp Arg Lys Ile Leu Glu 
        675                 680                 685             


Asp Lys Leu Arg Ala Asp Phe Ala Trp Leu Ser Glu Glu Gln Ile Lys 
    690                 695                 700                 


Gln Leu Cys Arg Leu Arg Leu Gln Gly Trp Gly Arg Leu Ser Asp Lys 
705                 710                 715                 720 


Leu Leu Thr Arg Leu Thr Asp Ala Asp Gly Gln Asn Val Leu Glu Arg 
                725                 730                 735     


Leu Trp Asn Ser Asn Asp Asn Phe Val Gln Ile Val Ser Asp Pro Asn 
            740                 745                 750         


Ile Lys Ala Lys Ile Glu Gln Glu Asn Gln Lys Leu Leu Arg Asp Ser 
        755                 760                 765             


Gln Ser Asp Asn Ala Val Glu Ser Ile Leu Asp Asp Ala Tyr Thr Ser 
    770                 775                 780                 


Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Met Arg Val Val His Asp 
785                 790                 795                 800 


Ile Val Lys Ala Cys Asp Gly Asn Val Pro Ala Lys Phe Ala Ile Glu 
                805                 810                 815     


Phe Ala Arg Ser Gln Glu Asp Asp Pro Lys Arg Thr Thr Ala Arg Val 
            820                 825                 830         


Asn Gln Met Gln Lys Ile Tyr Asp Gln Ile Ser Asp Glu Val Val Ser 
        835                 840                 845             


Gln Gly Val Lys Glu Gln Leu Ser Gly Met Lys Ser Leu Lys Asp Arg 
    850                 855                 860                 


Tyr Tyr Leu Tyr Phe Met Gln Gly Gly Arg Asp Ala Tyr Thr Gly Ala 
865                 870                 875                 880 


Lys Leu Asn Ile Asp Arg Leu Ser Asp Tyr Asp Val Asp His Ile Met 
                885                 890                 895     


Pro Gln Ser Phe Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val 
            900                 905                 910         


Ala Arg Ala Val Asn Asn Gln Lys Ser Asp Lys Val Pro Ala Leu Leu 
        915                 920                 925             


Phe Gly Asn Lys Val Val Ala Asp Leu Gly Ile Thr Val Arg Glu Met 
    930                 935                 940                 


Trp Asp Lys Trp Gln Lys Leu Gly Met Ile Ser Lys Arg Lys Leu Ser 
945                 950                 955                 960 


Asn Leu Leu Thr Asp Pro Asp Ala Leu Thr Glu Tyr Arg Ala Gln Gly 
                965                 970                 975     


Phe Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Thr 
            980                 985                 990         


Ala Thr Ile Leu Gln Ser Glu Phe  Pro Asp Ser Lys Ile  Ile Glu Val 
        995                 1000                 1005             


Pro Ala  Lys Tyr Asn Ser Ile  Val Arg Lys Gln Phe  Asp Leu Tyr 
    1010                 1015                 1020             


Lys Ser  Arg Glu Val Asn Asp  Phe His His Ala Ile  Asp Ala Tyr 
    1025                 1030                 1035             


Leu Ser  Thr Ile Val Gly Asn  Tyr Leu Tyr Gln Val  Tyr Pro Asn 
    1040                 1045                 1050             


Leu Arg  Arg Met Phe Val Tyr  Gly Glu Phe Lys Lys  Phe Ser Ser 
    1055                 1060                 1065             


Asn Ala  Glu Glu Ser Ala His  Asp Val Ala Arg Arg  Val Lys Ser 
    1070                 1075                 1080             


Met Asn  Phe Leu Asp Asp Leu  Leu Arg Gly Thr His  Gly Asp Asn 
    1085                 1090                 1095             


Ile Tyr  Cys Arg Ser Thr Gly  Glu Ile Val Phe Asn  Arg Asn Asp 
    1100                 1105                 1110             


Ile Ile  Ser Lys Leu Lys Gln  Ala Tyr Ser Phe Lys  Gln Met Leu 
    1115                 1120                 1125             


Val Thr  Gln Glu Val Phe Thr  Arg Lys Ser Ala Leu  Phe Asp Gln 
    1130                 1135                 1140             


Thr Val  Tyr Pro Ser Pro Glu  Arg Asp Ser Lys Lys  Arg Ser Gly 
    1145                 1150                 1155             


Leu Ile  Pro Arg Lys Lys Gly  Met Asp Pro Glu Ile  Tyr Gly Gly 
    1160                 1165                 1170             


Tyr Ala  His Asn Gln Asp Ser  Tyr Phe Val Leu Ala  Lys Ile Glu 
    1175                 1180                 1185             


Lys Val  Lys Gly Tyr Thr Leu  Gln Ile Val Gly Val  Pro Ile Arg 
    1190                 1195                 1200             


Ser Leu  Glu Ile Ile Asn Lys  Ala Glu Asp Phe Ser  Thr Ala Leu 
    1205                 1210                 1215             


Leu Asp  Ile Ile Lys Ser Lys  Val Met Tyr Asn Glu  Lys Thr Gly 
    1220                 1225                 1230             


Gln Pro  Lys Arg Gly Ile Lys  Asn Val Glu Ile Leu  Val Asn Lys 
    1235                 1240                 1245             


Leu Pro  Tyr Arg Gln Pro Ile  Leu Glu Lys Asp Val  Ser Tyr Met 
    1250                 1255                 1260             


Leu Gly  Ser Ser Ala Tyr Lys  Tyr Asn Met Lys Gln  Ile Thr Leu 
    1265                 1270                 1275             


Ser Arg  Gln Ser Met Lys Tyr  Ile Leu Asp Tyr Ile  Asp Asp Pro 
    1280                 1285                 1290             


Asn Phe  Thr Lys His Glu Met  Thr Met Ile Asp Glu  Gln Asn Glu 
    1295                 1300                 1305             


Arg Glu  Cys Leu Leu Ser Val  Tyr Asp Glu Ile Leu  Glu Lys Val 
    1310                 1315                 1320             


Asp Lys  Tyr Leu Pro Leu Phe  Asp Gly Asn Lys Phe  Arg Glu Lys 
    1325                 1330                 1335             


Leu His  Ser Ala Arg Lys Glu  Phe Val Ser Leu Ser  Val Asp Asp 
    1340                 1345                 1350             


Glu Ala  Ser Ala Ile Glu Lys  Ile Leu Arg Gly Leu  His Ala Asn 
    1355                 1360                 1365             


Ser Glu  Met Pro Ser Leu Glu  Asn Leu Lys Ile Lys  Pro Ala Leu 
    1370                 1375                 1380             


Gly Phe  Phe Gln Met Lys Asn  Gly Ile Thr Leu Ser  Asn Asp Ala 
    1385                 1390                 1395             


Val Leu  Leu Tyr Gln Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Val 
    1400                 1405                 1410             


Lys Val  Ser Asp Leu Leu Lys  
    1415                 1420 


<210>  1208
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1208

Met Ala Lys Pro Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Val Val Thr Asp Asp Gln Asn Asn Ile Leu Arg Ile 
            20                  25                  30          


Lys Gly Lys Lys Ala Ile Gly Ala Arg Leu Phe Thr Glu Gly Lys Val 
        35                  40                  45              


Ala Ala Lys Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg 
    50                  55                  60                  


Arg Arg Trp Arg Ile Lys Leu Leu Glu Glu Leu Phe Asp Glu Glu Ile 
65                  70                  75                  80  


Ala Lys Val Asp Pro Ser Phe Phe Ala Arg Leu His Glu Ser Trp Ile 
                85                  90                  95      


Ser Pro Lys Asp Lys Arg Lys Arg Tyr Ser Ala Ile Val Phe Pro Ser 
            100                 105                 110         


Pro Glu Glu Asp Lys Lys Phe His Glu Ser Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Asp Lys Leu Met Lys Asp Asp Gln Lys His Asp Ile Arg Glu 
    130                 135                 140                 


Ile Tyr Ile Ala Val His Gln Met Ile Lys Ala Arg Gly Asn Phe Leu 
145                 150                 155                 160 


His Asp Glu Ser Val Glu Thr Tyr Arg Ser Gly Met Ser Ser Leu Gly 
                165                 170                 175     


Gly Arg Ser Glu Arg Thr Ile Leu Ser Val Gln Thr Leu Glu Glu Leu 
            180                 185                 190         


Asn Asp Leu Phe Ala Glu Asn Glu Gly Thr Glu Glu Val Glu Leu Asn 
        195                 200                 205             


Val Ala Ser Ala Glu Gln Ile Asn Asp Ile Leu Thr Gly Gly His Leu 
    210                 215                 220                 


Asn Ala Asp Ser Gln Lys Glu Ile Ser Asn Leu Leu Leu Pro Ser Ser 
225                 230                 235                 240 


Phe Pro Ser Phe Asp Asp Lys Ala Lys Glu Lys Gln Val Lys Lys Leu 
                245                 250                 255     


Ile Asn Asn Val Ala Thr Asn Ile Ser Lys Ala Trp Leu Gly Tyr Lys 
            260                 265                 270         


Ala Asp Phe Ser Thr Ile Leu Asn Leu Ala Lys Val Asp Lys Asp Gln 
        275                 280                 285             


Lys Lys Ile Phe Ala Phe Ala Leu Gln Gly Glu Asp Glu Glu Asp Lys 
    290                 295                 300                 


Val Gln Glu Leu Glu Ser Leu Leu Glu Gln Ser Gln Thr Asp Ile Val 
305                 310                 315                 320 


Asp Arg Leu Ile Glu Ile Arg His Ala Ile Val Leu Ser Glu Ile Val 
                325                 330                 335     


Pro Val Gly Met Thr Leu Ser Glu Ala Met Ile Asp Lys Tyr Asp Gln 
            340                 345                 350         


His Lys Glu Asp Leu Ile Thr Leu Lys Ala Val Ile Arg Asn Thr Lys 
        355                 360                 365             


Asp Lys Lys Lys Ala Ala Lys Leu Gln Ala Ile Tyr Asp Leu Tyr Val 
    370                 375                 380                 


Lys Asn Arg His Ala Asp Leu Ala Lys Ala Met Lys Leu Thr Gly Ile 
385                 390                 395                 400 


Lys Lys Arg Ser Glu Leu Leu Asp Pro Glu Glu Leu Lys Lys Gly Ile 
                405                 410                 415     


Ser Ser Leu Leu Asp Asp Ser Pro Glu Ala Val Glu Ile Lys Gln Arg 
            420                 425                 430         


Ile Glu Glu Lys Thr Phe Leu Pro Leu Gln Arg Ser Asn Asn Asn Gly 
        435                 440                 445             


Val Ile Pro Asn Gln Leu His Gln Val Glu Leu Asp Glu Ile Ile Lys 
    450                 455                 460                 


Lys Gln Ser Lys Tyr Tyr Pro Phe Leu Ala Glu Lys Asn Pro Asp Glu 
465                 470                 475                 480 


Ser Glu Glu Ala Gln Lys Lys Ala Pro Thr Lys Leu Asp Ala Leu Leu 
                485                 490                 495     


Thr Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Lys Glu Glu 
            500                 505                 510         


Gln Glu Ala Gln Asn Gly His Ser Phe Ala Trp Met Val Arg Arg Asp 
        515                 520                 525             


Pro Gln Asp His Glu Ala Ile Thr Pro Trp Asn Phe Glu Lys Lys Val 
    530                 535                 540                 


Asp Lys Met Ala Ser Ala Thr Gln Phe Ile Lys Arg Met Thr Thr Lys 
545                 550                 555                 560 


Asp Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Ala Ser Ser Leu Lys 
                565                 570                 575     


Tyr Gln Leu Phe Thr Val Leu Asn Glu Leu Asn Asn Leu Arg Val Asn 
            580                 585                 590         


Gly Lys Lys Leu Thr Ser Asp Glu Lys Glu Gln Val Ile Glu Gly Leu 
        595                 600                 605             


Phe Lys Lys Gln Lys Thr Val Lys Ala Asp Lys Phe Val Lys Tyr Trp 
    610                 615                 620                 


Gln Ala Lys His Ile Gly Ala Asp Ile Lys Val Lys Gly Leu Ser Asp 
625                 630                 635                 640 


Pro Ser Lys Phe Asn Ser Thr Met Ser Thr Tyr Ile Asp Phe Lys Lys 
                645                 650                 655     


Ile Phe Gly Asp Gln Leu Asn Asp Val Asn Arg Gln Asn Asp Phe Glu 
            660                 665                 670         


Lys Ile Ile Glu Tyr Ser Thr Ile Phe Glu Asp Arg Lys Ile Leu Glu 
        675                 680                 685             


Asp Lys Leu Arg Val Asp Phe Ala Trp Leu Ser Glu Glu Gln Ile Lys 
    690                 695                 700                 


Gln Leu Cys Arg Leu Arg Leu Gln Gly Trp Gly Arg Leu Ser Asp Lys 
705                 710                 715                 720 


Leu Leu Thr Arg Leu Thr Asp Ala Asp Gly Gln Asn Val Leu Glu Arg 
                725                 730                 735     


Leu Trp Asn Ser Asn Asp Asn Phe Val Gln Ile Val Ser Asp Pro Asn 
            740                 745                 750         


Ile Lys Ala Lys Ile Glu Gln Glu Asn Gln Lys Leu Leu Arg Asp Ser 
        755                 760                 765             


Gln Ser Asp Asn Ala Val Glu Ser Ile Leu Asp Asp Ala Tyr Thr Ser 
    770                 775                 780                 


Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Met Arg Val Val His Asp 
785                 790                 795                 800 


Ile Val Lys Ala Cys Asp Gly Asn Val Pro Ala Lys Phe Ala Ile Glu 
                805                 810                 815     


Phe Ala Arg Ser Gln Glu Asp Asp Pro Lys Arg Thr Thr Ala Arg Val 
            820                 825                 830         


Asn Gln Met Gln Lys Ile Tyr Asp Gln Ile Ser Asp Glu Val Val Ser 
        835                 840                 845             


Gln Gly Val Lys Glu Gln Leu Ser Gly Met Lys Ser Leu Lys Asp Arg 
    850                 855                 860                 


Tyr Tyr Leu Tyr Phe Met Gln Gly Gly Arg Asp Ala Tyr Thr Gly Ala 
865                 870                 875                 880 


Lys Leu Asn Ile Asp Arg Leu Ser Asp Tyr Asp Val Asp His Ile Met 
                885                 890                 895     


Pro Gln Ser Phe Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val 
            900                 905                 910         


Ala Arg Ala Val Asn Asn Gln Lys Ser Asp Lys Val Pro Ala Leu Leu 
        915                 920                 925             


Phe Gly Asn Lys Val Val Ala Asp Leu Gly Ile Thr Val Arg Glu Met 
    930                 935                 940                 


Trp Asp Lys Trp Gln Lys Leu Gly Met Ile Ser Lys Arg Lys Leu Ser 
945                 950                 955                 960 


Asn Leu Leu Thr Asp Pro Asp Ala Leu Thr Glu Tyr Arg Ala Gln Gly 
                965                 970                 975     


Phe Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Thr 
            980                 985                 990         


Ala Thr Ile Leu Gln Ser Glu Phe  Pro Asp Ser Lys Ile  Ile Glu Val 
        995                 1000                 1005             


Pro Ala  Lys Tyr Asn Ser Ile  Val Arg Lys Gln Phe  Asp Leu Tyr 
    1010                 1015                 1020             


Lys Ser  Arg Glu Val Asn Asp  Phe His His Ala Ile  Asp Ala Tyr 
    1025                 1030                 1035             


Leu Ser  Thr Ile Val Gly Asn  Tyr Leu Tyr Gln Val  Tyr Pro Asn 
    1040                 1045                 1050             


Leu Arg  Arg Met Phe Val Tyr  Gly Glu Phe Lys Lys  Phe Ser Ser 
    1055                 1060                 1065             


Asn Ala  Glu Glu Ser Ala His  Asp Val Ala Arg Arg  Val Lys Ser 
    1070                 1075                 1080             


Met Asn  Phe Leu Asp Asp Leu  Leu Arg Gly Thr His  Gly Asp Asn 
    1085                 1090                 1095             


Ile Tyr  Cys Arg Ser Thr Gly  Glu Ile Val Phe Asn  Arg Asn Asp 
    1100                 1105                 1110             


Ile Ile  Ser Lys Leu Lys Gln  Ala Tyr Ser Phe Lys  Gln Met Leu 
    1115                 1120                 1125             


Val Thr  Gln Glu Val Phe Thr  Lys Lys Ser Ala Leu  Phe Asn Gln 
    1130                 1135                 1140             


Thr Val  Tyr Pro Ser Pro Lys  Arg Asp Gly Ser Lys  Arg Ser Gly 
    1145                 1150                 1155             


Leu Ile  Pro Arg Lys Arg Gly  Met Asp Pro Glu Ile  Tyr Gly Gly 
    1160                 1165                 1170             


Tyr Ala  His Asn Gln Asp Ser  His Phe Val Leu Ala  Lys Ile Glu 
    1175                 1180                 1185             


Lys Val  Lys Gly Tyr Thr Leu  Gln Ile Val Gly Val  Pro Ile Arg 
    1190                 1195                 1200             


Ser Leu  Glu Ile Ile Asn Lys  Ala Glu Asp Phe Ser  Thr Ala Leu 
    1205                 1210                 1215             


Leu Asp  Ile Ile Lys Ser Lys  Val Met Tyr Asn Glu  Lys Thr Gly 
    1220                 1225                 1230             


Gln Pro  Lys Arg Gly Ile Lys  Asn Val Glu Ile Leu  Val Asn Lys 
    1235                 1240                 1245             


Leu Pro  Tyr Arg Gln Pro Ile  Leu Glu Lys Asp Val  Ser Tyr Met 
    1250                 1255                 1260             


Leu Gly  Ser Ser Ala Tyr Lys  Tyr Asn Ile Lys Gln  Ile Thr Leu 
    1265                 1270                 1275             


Ser Arg  Gln Ser Met Lys Tyr  Ile Leu Asp Tyr Ile  Asp Asp Pro 
    1280                 1285                 1290             


Asn Phe  Thr Lys His Glu Met  Thr Met Ile Asp Glu  Gln Asn Glu 
    1295                 1300                 1305             


Arg Glu  Cys Leu Leu Ser Val  Tyr Asp Glu Ile Leu  Glu Lys Val 
    1310                 1315                 1320             


Asp Lys  Tyr Leu Pro Leu Phe  Asp Gly Asn Lys Phe  Arg Glu Lys 
    1325                 1330                 1335             


Leu His  Ser Ala Arg Lys Glu  Phe Val Ser Leu Ser  Val Asp Asp 
    1340                 1345                 1350             


Glu Ala  Ser Ala Ile Glu Lys  Ile Leu Arg Gly Leu  His Ala Asn 
    1355                 1360                 1365             


Ser Glu  Met Pro Ser Leu Glu  Asn Leu Lys Ile Lys  Pro Ala Leu 
    1370                 1375                 1380             


Gly Phe  Phe Gln Met Lys Asn  Gly Ile Thr Leu Ser  Asn Asp Ala 
    1385                 1390                 1395             


Val Leu  Leu Tyr Gln Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Val 
    1400                 1405                 1410             


Lys Val  Ser Asp Leu Leu Lys  
    1415                 1420 


<210>  1209
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1209

Met Ala Lys Phe Asn Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Val 
1               5                   10                  15      


Ser Ser Val Gly Tyr Ala Val Val Thr Asp Asp Tyr Arg Val Pro Ala 
            20                  25                  30          


Phe Lys Phe Lys Val Leu Gly Asn Thr Glu Lys Glu Lys Ile Lys Lys 
        35                  40                  45              


Asn Leu Ile Gly Ser Thr Thr Phe Val Pro Ala Gln Ser Ala Gln Gly 
    50                  55                  60                  


Thr Arg Val Phe Arg Val Asn Arg Arg Arg Ile Asp Arg Arg Asn His 
65                  70                  75                  80  


Arg Ile Ala Tyr Leu Arg Asp Ile Phe Gln Lys Glu Ile Glu Lys Val 
                85                  90                  95      


Asp Lys Asn Phe Tyr Arg Arg Leu Asp Glu Ser Phe Arg Val Leu Gly 
            100                 105                 110         


Asp Lys Ser Glu Glu Leu Gln Ile Lys Gln Pro Phe Phe Gly Asp Lys 
        115                 120                 125             


Glu Leu Glu Thr Ala Tyr His Lys Lys Tyr Pro Thr Ile Tyr His Leu 
    130                 135                 140                 


Arg Lys His Leu Ala Asp Ala Asp Lys Asn Ser Pro Val Ala Asp Ile 
145                 150                 155                 160 


Arg Glu Val Tyr Met Ala Ile Ser His Ile Leu Lys Tyr Arg Gly His 
                165                 170                 175     


Phe Leu Thr Leu Asp Arg Ile Asn Pro Asn Asn Ile Asn Met Gln Asn 
            180                 185                 190         


Ser Trp Ile Asp Phe Ile Glu Ser Cys Gln Glu Val Phe Asp Leu Glu 
        195                 200                 205             


Ile Ser Asp Glu Ser Lys Thr Ile Val Ala Ile Phe Lys Ser Ser Asp 
    210                 215                 220                 


Asn Arg Gln Glu Lys Val Lys Lys Ile Leu Ser Tyr Phe Gln Gln Glu 
225                 230                 235                 240 


Leu Leu Lys Lys Asp Lys Ser Ile Phe Lys Gln Leu Leu Gln Leu Leu 
                245                 250                 255     


Phe Gly Leu Lys Thr Lys Phe Lys Asp Cys Phe Glu Leu Glu Glu Glu 
            260                 265                 270         


Pro Asp Leu Asn Phe Ser Lys Glu Asn Tyr Asp Glu Asn Leu Glu Asn 
        275                 280                 285             


Phe Leu Gly Ser Leu Glu Glu Asp Phe Ser Asp Val Phe Ala Lys Leu 
    290                 295                 300                 


Lys Val Leu Arg Asp Thr Ile Leu Leu Ser Gly Met Leu Thr Tyr Thr 
305                 310                 315                 320 


Gly Thr Thr Arg Ala Arg Phe Ser Ala Thr Met Val Glu Arg Tyr Glu 
                325                 330                 335     


Glu His Arg Lys Asp Leu Gln Arg Phe Lys Ser Phe Val Lys Gln Asn 
            340                 345                 350         


Leu Ser Glu Gln Asp Tyr Leu Asp Ile Phe Gly Arg Lys Thr Pro Asn 
        355                 360                 365             


Gly Phe Asp Val Asp Lys Glu Thr Lys Gly Tyr Val Gly Tyr Ile Ser 
    370                 375                 380                 


Asn Lys Met Val Leu Thr Asn Pro Gln Lys Gln Lys Thr Ile Gln Gln 
385                 390                 395                 400 


Asn Phe Tyr Asp Tyr Ile Ser Gly Lys Ile Thr Gly Ile Glu Gly Ala 
                405                 410                 415     


Glu Tyr Phe Leu Asn Lys Ile Ser Asp Gly Thr Phe Leu Arg Lys Leu 
            420                 425                 430         


Arg Thr Thr Asp Asn Gly Ala Val Pro Asn Gln Ile His Ala Tyr Glu 
        435                 440                 445             


Leu Glu Lys Ile Ile Asp Arg Gln Gly Glu Asp Tyr Pro Phe Leu Leu 
    450                 455                 460                 


Glu Asn Lys Asp Lys Leu Leu Ser Ile Leu Thr Phe Lys Ile Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ser Lys Gly Asn Asn Ser Arg Phe Gly Trp Ile 
                485                 490                 495     


Lys Arg Thr Ile Ser Gln Asp Ile Leu Asp Asp Asn Asp Glu Asp Thr 
            500                 505                 510         


Arg Asn Gly Lys Ile Arg Pro Trp Asn Tyr Gln Lys Leu Ile Asn Met 
        515                 520                 525             


Asp Glu Thr Arg Asp Ala Phe Ile Thr Asn Leu Ile Gly Asn Asp Ile 
    530                 535                 540                 


Ile Leu Leu Asn Glu Lys Val Leu Pro Lys Arg Ser Leu Ile Tyr Glu 
545                 550                 555                 560 


Glu Val Met Leu Gln Asn Glu Leu Thr Arg Val Lys Tyr Lys Asp Lys 
                565                 570                 575     


Tyr Gly Lys Thr His Phe Phe Asp Ser Glu Leu Arg Gln Glu Ile Ile 
            580                 585                 590         


Asn Asn Leu Phe Lys Thr Asn Asn Lys Arg Val Ser Ser Ala Met Leu 
        595                 600                 605             


Leu Asp Tyr Leu Glu Asn Tyr Thr Asn Leu Gln Ala Val Glu Ile Val 
    610                 615                 620                 


Ser Gly Ile Glu Lys Gly Lys Ser Leu Asn Ser Thr Leu Lys Thr Tyr 
625                 630                 635                 640 


Asn Asp Leu Lys Thr Ile Phe Ser Glu Glu Leu Leu Asp Ser Glu Ile 
                645                 650                 655     


Tyr Gln Lys Glu Leu Glu Glu Ile Val Lys Val Ile Thr Val Phe Asp 
            660                 665                 670         


Asp Lys Lys Ser Ile Lys Asn Tyr Leu Ile Lys Tyr Phe Gly His Leu 
        675                 680                 685             


Glu Phe Leu Asn Glu Glu Arg Ile Asn Gln Leu Ser Lys Leu Arg Tyr 
    690                 695                 700                 


Thr Gly Trp Gly Arg Tyr Ser Ala Lys Leu Leu Leu Asp Ile Arg Asp 
705                 710                 715                 720 


Glu Asp Thr Gly Phe Asn Leu Met Gln Phe Leu Arg Asn Asp Asp Glu 
                725                 730                 735     


Asn Arg Asn Leu Ser Gln Leu Ile Ser Asp Ser Thr Leu Ser Phe Asp 
            740                 745                 750         


Ser Ala Ile Lys Ser Ile Gln Ser Lys Ser Thr Val Glu Asp Asp Val 
        755                 760                 765             


Phe Asp Glu Ile Lys Lys Leu Ala Gly Ser Pro Ala Ile Lys Arg Gly 
    770                 775                 780                 


Ile Leu Asn Ser Ile Lys Ile Val Asp Glu Leu Val Gln Ile Ile Gly 
785                 790                 795                 800 


Tyr Pro Pro Gln Asn Ile Val Ile Glu Met Ala Arg Glu Asn Met Thr 
                805                 810                 815     


Thr Glu Glu Gly Gln Lys Lys Ala Lys Thr Arg Lys Thr Lys Leu Glu 
            820                 825                 830         


Ser Ala Leu Lys Asn Ile Glu Asn Ser Val Leu Glu Asn Gly Lys Val 
        835                 840                 845             


Pro His Ser Asp Glu Gln Leu Gln Ser Glu Lys Leu Tyr Leu Tyr Tyr 
    850                 855                 860                 


Leu Gln Asn Gly Lys Asp Met Tyr Ser Leu Asp Lys Thr Gly Ser Pro 
865                 870                 875                 880 


Thr Pro Leu Tyr Arg Asp Gln Leu Asp Gln Tyr Glu Val Asp His Ile 
                885                 890                 895     


Ile Pro Tyr Ser Phe Leu Pro Ile Asp Ser Ile Asp Asn Lys Val Leu 
            900                 905                 910         


Thr His Arg Glu Asn Asn Gln Arg Lys Leu Asn Asn Ile Pro Asp Lys 
        915                 920                 925             


Glu Thr Val Ala Asn Met Lys Pro Phe Trp Glu Lys Leu Tyr Asn Ala 
    930                 935                 940                 


Lys Leu Ile Ser Gln Thr Lys Tyr Gln Arg Leu Thr Thr Ser Glu Arg 
945                 950                 955                 960 


Thr Pro Asp Gly Val Leu Thr Glu Ser Met Lys Ala Gly Phe Ile Glu 
                965                 970                 975     


Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys His Val Ala Arg Ile 
            980                 985                 990         


Leu Asp Asn Arg Phe Leu Asp Thr  Lys Ile Ile Ala Leu  Lys Ser Gln 
        995                 1000                 1005             


Leu Ile  Thr Asn Phe Arg Asn  Thr Phe His Ile Ala  Lys Ile Arg 
    1010                 1015                 1020             


Glu Leu  Asn Asp Tyr His His  Ala His Asp Ala Tyr  Leu Ala Val 
    1025                 1030                 1035             


Val Val  Gly Gln Thr Leu Leu  Lys Ala Tyr Pro Lys  Leu Ala Pro 
    1040                 1045                 1050             


Glu Leu  Ile Tyr Gly His His  Ala His Phe Asn Arg  His Glu Glu 
    1055                 1060                 1065             


Asn Lys  Ala Thr Ile Arg Lys  His Leu Tyr Ser Asn  Ile Met Arg 
    1070                 1075                 1080             


Phe Phe  Asn Asn Pro Asp Ser  Lys Val Ser Lys Asp  Ile Trp Asp 
    1085                 1090                 1095             


Cys Asn  Arg Asp Leu Pro Ile  Ile Lys Glu Val Ile  Tyr Asn Ser 
    1100                 1105                 1110             


Gln Val  Asn Phe Val Lys Arg  Thr Met Ile Lys Lys  Gly Ala Phe 
    1115                 1120                 1125             


Tyr Asn  Gln Asn Pro Val Gly  Lys Phe Asn Lys Gln  Ile Ala Ala 
    1130                 1135                 1140             


Asn Asn  Arg Tyr Pro Leu Lys  Thr Lys Val Ser Ser  Leu Asp Thr 
    1145                 1150                 1155             


Ser Ile  Tyr Gly Gly Tyr Gly  Pro Met Asn Ser Ala  Leu Ser Ile 
    1160                 1165                 1170             


Ile Ile  Ile Ala Glu Arg Phe  Asn Glu Lys Lys Gly  Lys Ile Glu 
    1175                 1180                 1185             


Thr Val  Lys Glu Phe His Asp  Ile Phe Ile Ile Asp  Tyr Lys Lys 
    1190                 1195                 1200             


Phe Asn  Asn Asn Pro Phe Gln  Phe Leu Asn Asp Thr  Ser Glu Asn 
    1205                 1210                 1215             


Gly Phe  Leu Lys Lys Asn Asn  Ile Asn Arg Val Leu  Ala Phe Tyr 
    1220                 1225                 1230             


Gly Val  Pro Lys Tyr Ser Leu  Met Gln Lys Asn Asp  Gly Thr Arg 
    1235                 1240                 1245             


Met Leu  Phe Glu Ser Lys Ser  Asn Leu His Lys Ala  Thr Gln Phe 
    1250                 1255                 1260             


Lys Leu  Thr Lys Thr Gln Asn  Glu Leu Phe Phe His  Met Arg Arg 
    1265                 1270                 1275             


Leu Leu  Thr Lys Ser Asn Leu  Met Asp Leu Lys Ser  Glu Ser Ala 
    1280                 1285                 1290             


Ile Lys  Glu Ser Gln Asn Phe  Ile Leu Lys His Lys  Glu Glu Phe 
    1295                 1300                 1305             


Asp Asn  Ile Ser Asn Gln Leu  Ser Ala Phe Ser Gln  Lys Met Leu 
    1310                 1315                 1320             


Gly Asp  Thr Thr Ser Leu Arg  Asn Leu Ile Lys Gly  Tyr Asn Glu 
    1325                 1330                 1335             


Arg Lys  Ile Lys Glu Ile Asp  Ser Ser Glu Glu Val  Ile Lys Tyr 
    1340                 1345                 1350             


Phe Tyr  Asp Asn Phe Ile Lys  Met Phe Ser Phe Val  Lys Ser Gly 
    1355                 1360                 1365             


Ala Pro  Lys Gly Ile Asn Asp  Phe Phe Asn Asn Lys  Tyr Thr Val 
    1370                 1375                 1380             


Ala Arg  Met Arg Pro Lys Pro  Asp Lys Lys Phe Leu  Asn Ala Thr 
    1385                 1390                 1395             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1400                 1405                 1410             


Leu Ser  Lys Leu Gly Glu Asp  
    1415                 1420 


<210>  1210
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1210

Met Ala Lys Phe Asn Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Val 
1               5                   10                  15      


Ser Ser Val Gly Tyr Ala Val Val Thr Asp Asp Tyr Arg Val Pro Ala 
            20                  25                  30          


Phe Lys Phe Lys Val Leu Gly Asn Thr Glu Lys Glu Lys Ile Lys Lys 
        35                  40                  45              


Asn Leu Ile Gly Ser Thr Thr Phe Val Pro Ala Gln Ser Ala Gln Gly 
    50                  55                  60                  


Thr Arg Val Phe Arg Val Asn Arg Arg Arg Ile Asp Arg Arg Asn His 
65                  70                  75                  80  


Arg Ile Ala Tyr Leu Arg Asp Ile Phe Gln Lys Glu Ile Glu Lys Val 
                85                  90                  95      


Asp Lys Asn Phe Tyr Arg Arg Leu Asp Glu Ser Phe Arg Val Leu Gly 
            100                 105                 110         


Asp Lys Ser Glu Glu Leu Gln Ile Lys Gln Pro Phe Phe Gly Asp Lys 
        115                 120                 125             


Glu Leu Glu Thr Ala Tyr His Lys Lys Tyr Pro Thr Ile Tyr His Leu 
    130                 135                 140                 


Arg Lys His Leu Ala Asp Ala Asp Lys Asn Ser Pro Val Ala Asp Ile 
145                 150                 155                 160 


Arg Glu Val Tyr Met Ala Ile Ser His Ile Leu Lys Tyr Arg Gly His 
                165                 170                 175     


Phe Leu Thr Leu Asp Arg Ile Asn Pro Asn Asn Ile Asn Met Gln Asn 
            180                 185                 190         


Ser Trp Ile Asp Phe Ile Glu Ser Cys Gln Glu Val Phe Asp Leu Glu 
        195                 200                 205             


Ile Ser Asp Glu Ser Lys Thr Ile Val Ala Ile Phe Lys Ser Ser Asp 
    210                 215                 220                 


Asn Arg Gln Glu Lys Val Lys Lys Ile Leu Ser Tyr Phe Gln Gln Glu 
225                 230                 235                 240 


Leu Leu Lys Lys Asp Lys Ser Ile Phe Lys Gln Leu Leu Gln Leu Leu 
                245                 250                 255     


Phe Gly Leu Lys Thr Lys Phe Lys Asp Cys Phe Glu Leu Glu Glu Glu 
            260                 265                 270         


Pro Asp Leu Asn Phe Ser Lys Glu Asn Tyr Asp Glu Asn Leu Glu Asn 
        275                 280                 285             


Phe Leu Gly Ser Leu Glu Glu Asp Phe Ser Asp Val Phe Ala Lys Leu 
    290                 295                 300                 


Lys Val Leu Arg Asp Thr Ile Leu Leu Ser Gly Met Leu Thr Tyr Thr 
305                 310                 315                 320 


Gly Thr Thr Arg Ala Arg Phe Ser Ala Thr Met Val Glu Arg Tyr Glu 
                325                 330                 335     


Glu His Arg Lys Asp Leu Gln Arg Phe Lys Ser Phe Val Lys Gln Asn 
            340                 345                 350         


Leu Ser Glu Gln Asp Tyr Leu Asp Ile Phe Gly Arg Lys Thr Pro Asn 
        355                 360                 365             


Gly Phe Asp Val Asp Lys Glu Thr Lys Gly Tyr Val Gly Tyr Ile Ser 
    370                 375                 380                 


Asn Lys Met Val Leu Thr Asn Pro Gln Lys Gln Lys Thr Ile Gln Gln 
385                 390                 395                 400 


Asn Phe Tyr Asp Tyr Ile Ser Gly Lys Ile Thr Gly Ile Glu Gly Ala 
                405                 410                 415     


Glu Tyr Phe Leu Asn Lys Ile Ser Asp Gly Thr Phe Leu Arg Lys Leu 
            420                 425                 430         


Arg Thr Thr Asp Asn Gly Ala Val Pro Asn Gln Ile His Ala Tyr Glu 
        435                 440                 445             


Leu Glu Lys Ile Ile Asp Arg Gln Gly Glu Asp Tyr Pro Phe Leu Leu 
    450                 455                 460                 


Glu Asn Lys Asp Lys Leu Leu Ser Ile Leu Thr Phe Lys Ile Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ser Lys Gly Asn Asn Ser Arg Phe Gly Trp Ile 
                485                 490                 495     


Lys Arg Thr Ile Ser Gln Asp Ile Leu Asp Asp Asn Asp Glu Asp Thr 
            500                 505                 510         


Arg Asn Gly Lys Ile Arg Pro Trp Asn Tyr Gln Lys Leu Ile Asn Met 
        515                 520                 525             


Asp Glu Thr Arg Asp Ala Phe Ile Thr Asn Leu Ile Gly Asn Asp Ile 
    530                 535                 540                 


Ile Leu Leu Asn Glu Lys Val Leu Pro Lys Arg Ser Leu Ile Tyr Glu 
545                 550                 555                 560 


Glu Val Met Leu Gln Asn Glu Leu Thr Arg Val Lys Tyr Lys Asp Lys 
                565                 570                 575     


Tyr Gly Lys Thr His Phe Phe Asp Ser Glu Leu Arg Gln Glu Ile Ile 
            580                 585                 590         


Asn Asn Leu Phe Lys Thr Asn Asn Lys Arg Val Ser Ser Ala Met Leu 
        595                 600                 605             


Leu Asp Tyr Leu Glu Asn Tyr Thr Asn Leu Gln Ala Val Glu Ile Val 
    610                 615                 620                 


Ser Gly Ile Glu Lys Gly Lys Ser Leu Asn Ser Thr Leu Lys Thr Tyr 
625                 630                 635                 640 


Asn Asp Leu Lys Thr Ile Phe Ser Glu Glu Leu Leu Asp Ser Glu Ile 
                645                 650                 655     


Tyr Gln Lys Glu Leu Glu Glu Ile Val Lys Val Ile Thr Val Phe Asp 
            660                 665                 670         


Asp Lys Lys Ser Ile Lys Asn Tyr Leu Ile Lys Tyr Phe Gly His Leu 
        675                 680                 685             


Glu Phe Leu Asn Glu Glu Arg Ile Asn Gln Leu Ser Lys Leu Arg Tyr 
    690                 695                 700                 


Thr Gly Trp Gly Arg Tyr Ser Ala Lys Leu Leu Leu Asp Ile Arg Asp 
705                 710                 715                 720 


Glu Asp Thr Gly Phe Asn Leu Met Gln Phe Leu Arg Asn Asp Asp Glu 
                725                 730                 735     


Asn Arg Asn Leu Ser Gln Leu Ile Ser Asp Ser Thr Leu Ser Phe Asp 
            740                 745                 750         


Ser Ala Ile Lys Thr Ile Gln Ser Lys Ser Thr Val Glu Asp Asp Val 
        755                 760                 765             


Phe Asp Glu Ile Lys Lys Leu Ala Gly Ser Pro Ala Ile Lys Arg Gly 
    770                 775                 780                 


Ile Leu Asn Ser Ile Lys Ile Val Asp Glu Leu Val Gln Ile Ile Gly 
785                 790                 795                 800 


Tyr Pro Pro Gln Asn Ile Val Ile Glu Met Ala Arg Glu Asn Met Thr 
                805                 810                 815     


Thr Glu Glu Gly Gln Lys Lys Ala Lys Thr Arg Lys Thr Lys Leu Glu 
            820                 825                 830         


Ser Ala Leu Lys Asn Ile Glu Asn Ser Val Leu Glu Asn Gly Lys Val 
        835                 840                 845             


Pro His Ser Asp Glu Gln Leu Gln Ser Glu Lys Leu Tyr Leu Tyr Tyr 
    850                 855                 860                 


Leu Gln Asn Gly Lys Asp Met Tyr Ser Leu Asp Lys Thr Gly Ser Pro 
865                 870                 875                 880 


Thr Pro Leu Tyr Arg Asp Gln Leu Asp Gln Tyr Glu Val Asp His Ile 
                885                 890                 895     


Ile Pro Tyr Ser Phe Leu Pro Ile Asp Ser Ile Asp Asn Lys Val Leu 
            900                 905                 910         


Thr His Arg Glu Asn Asn Gln Arg Lys Leu Asn Asn Ile Pro Asp Lys 
        915                 920                 925             


Glu Thr Val Ala Asn Met Lys Pro Phe Trp Glu Lys Leu Tyr Asn Ala 
    930                 935                 940                 


Lys Leu Ile Ser Gln Thr Lys Tyr Gln Arg Leu Thr Thr Ser Glu Arg 
945                 950                 955                 960 


Thr Pro Asp Gly Val Leu Thr Glu Ser Met Lys Ala Gly Phe Ile Glu 
                965                 970                 975     


Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys His Val Ala Arg Ile 
            980                 985                 990         


Leu Asp Asn Arg Phe Leu Asp Thr  Lys Ile Ile Ala Leu  Lys Ser Gln 
        995                 1000                 1005             


Leu Ile  Thr Asn Phe Arg Asn  Thr Phe His Ile Ala  Lys Ile Arg 
    1010                 1015                 1020             


Glu Leu  Asn Asp Tyr His His  Ala His Asp Ala Tyr  Leu Ala Val 
    1025                 1030                 1035             


Val Val  Gly Gln Thr Leu Leu  Lys Ala Tyr Pro Lys  Leu Ala Pro 
    1040                 1045                 1050             


Glu Leu  Ile Tyr Gly His His  Ala His Phe Asn Arg  His Glu Glu 
    1055                 1060                 1065             


Asn Lys  Ala Thr Ile Arg Lys  His Leu Tyr Ser Asn  Ile Met Arg 
    1070                 1075                 1080             


Phe Phe  Asn Asn Pro Asp Ser  Lys Val Ser Lys Asp  Ile Trp Asp 
    1085                 1090                 1095             


Cys Asn  Arg Asp Leu Pro Ile  Ile Lys Glu Val Ile  Tyr Asn Ser 
    1100                 1105                 1110             


Gln Val  Asn Phe Val Lys Arg  Thr Met Ile Lys Lys  Gly Ala Phe 
    1115                 1120                 1125             


Tyr Asn  Gln Asn Pro Val Gly  Lys Phe Asn Lys Gln  Ile Ala Ala 
    1130                 1135                 1140             


Asn Asn  Arg Tyr Pro Leu Lys  Thr Lys Val Ser Ser  Leu Asp Thr 
    1145                 1150                 1155             


Ser Ile  Tyr Gly Gly Tyr Gly  Pro Met Asn Ser Ala  Leu Ser Ile 
    1160                 1165                 1170             


Ile Ile  Ile Ala Glu Arg Phe  Asn Glu Lys Lys Gly  Lys Ile Glu 
    1175                 1180                 1185             


Thr Val  Lys Glu Phe His Asp  Ile Phe Ile Ile Asp  Tyr Lys Lys 
    1190                 1195                 1200             


Phe Asn  Asn Asn Pro Phe Gln  Phe Leu Asn Asp Thr  Ser Glu Asn 
    1205                 1210                 1215             


Gly Phe  Leu Lys Lys Asn Asn  Ile Asn Arg Val Leu  Ala Phe Tyr 
    1220                 1225                 1230             


Gly Val  Pro Lys Tyr Ser Leu  Met Gln Lys Asn Asp  Gly Thr Arg 
    1235                 1240                 1245             


Met Leu  Phe Glu Ser Lys Ser  Asn Leu His Lys Ala  Thr Gln Phe 
    1250                 1255                 1260             


Lys Leu  Thr Lys Thr Gln Asn  Glu Leu Phe Phe His  Met Arg Arg 
    1265                 1270                 1275             


Leu Leu  Thr Lys Ser Asn Leu  Met Asp Leu Lys Ser  Glu Ser Ala 
    1280                 1285                 1290             


Ile Lys  Glu Ser Gln Asn Phe  Ile Leu Lys His Lys  Glu Glu Phe 
    1295                 1300                 1305             


Asp Asn  Ile Ser Asn Gln Leu  Ser Ala Phe Ser Gln  Lys Met Leu 
    1310                 1315                 1320             


Gly Asp  Thr Thr Ser Leu Arg  Asn Leu Ile Lys Gly  Tyr Asn Glu 
    1325                 1330                 1335             


Arg Lys  Ile Lys Glu Ile Asp  Ser Ser Glu Glu Val  Ile Lys Tyr 
    1340                 1345                 1350             


Phe Tyr  Asp Asn Phe Ile Lys  Met Phe Ser Phe Val  Lys Ser Gly 
    1355                 1360                 1365             


Ala Pro  Lys Asp Ile Asn Asp  Phe Phe Asn Asn Lys  Tyr Thr Val 
    1370                 1375                 1380             


Ala Arg  Met Arg Pro Lys Pro  Asp Lys Lys Phe Leu  Asn Ala Thr 
    1385                 1390                 1395             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1400                 1405                 1410             


Leu Ser  Lys Leu Gly Glu Asp  
    1415                 1420 


<210>  1211
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1211

Met Ala Lys Phe Asn Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Val 
1               5                   10                  15      


Ser Ser Val Gly Tyr Ala Val Val Thr Asp Asp Tyr Arg Val Pro Ala 
            20                  25                  30          


Phe Lys Phe Lys Val Leu Gly Asn Thr Glu Lys Glu Lys Ile Lys Lys 
        35                  40                  45              


Asn Leu Ile Gly Ser Thr Thr Phe Val Pro Ala Gln Ser Ala Gln Gly 
    50                  55                  60                  


Thr Arg Val Phe Arg Val Asn Arg Arg Arg Ile Asp Arg Arg Asn His 
65                  70                  75                  80  


Arg Ile Ala Tyr Leu Arg Asp Ile Phe Gln Lys Glu Ile Glu Lys Val 
                85                  90                  95      


Asp Lys Asn Phe Tyr Arg Arg Leu Asp Glu Ser Phe Arg Val Leu Gly 
            100                 105                 110         


Asp Lys Ser Glu Glu Leu Gln Ile Lys Gln Pro Phe Phe Gly Asp Lys 
        115                 120                 125             


Glu Leu Glu Thr Ala Tyr His Lys Lys Tyr Pro Thr Ile Tyr His Leu 
    130                 135                 140                 


Arg Lys His Leu Ala Asp Ala Asp Lys Asn Ser Pro Val Ala Asp Ile 
145                 150                 155                 160 


Arg Glu Val Tyr Met Ala Ile Ser His Ile Leu Lys Tyr Arg Gly His 
                165                 170                 175     


Phe Leu Thr Leu Asp Arg Ile Asn Pro Asn Asn Ile Asn Met Gln Asn 
            180                 185                 190         


Ser Trp Ile Asp Phe Ile Glu Ser Cys Gln Glu Val Phe Asp Leu Glu 
        195                 200                 205             


Ile Ser Asp Glu Ser Lys Thr Ile Val Ala Ile Phe Lys Ser Ser Asp 
    210                 215                 220                 


Asn Arg Gln Glu Lys Val Lys Lys Ile Leu Ser Tyr Phe Gln Gln Glu 
225                 230                 235                 240 


Leu Leu Lys Lys Asp Lys Ser Ile Phe Lys Gln Leu Leu Gln Leu Leu 
                245                 250                 255     


Phe Gly Leu Lys Thr Lys Phe Lys Asp Cys Phe Glu Leu Glu Glu Glu 
            260                 265                 270         


Pro Asp Leu Asn Phe Ser Lys Glu Asn Tyr Asp Glu Asn Leu Glu Asn 
        275                 280                 285             


Phe Leu Gly Ser Leu Glu Glu Asp Phe Ser Asp Val Phe Ala Lys Leu 
    290                 295                 300                 


Lys Val Leu Arg Asp Thr Ile Leu Leu Ser Gly Met Leu Thr Tyr Thr 
305                 310                 315                 320 


Gly Thr Thr Arg Ala Arg Phe Ser Ala Thr Met Val Glu Arg Tyr Glu 
                325                 330                 335     


Glu His Arg Lys Asp Leu Gln Arg Phe Lys Ser Phe Val Lys Gln Asn 
            340                 345                 350         


Leu Ser Glu Gln Asp Tyr Leu Asp Ile Phe Gly Arg Lys Thr Pro Asn 
        355                 360                 365             


Gly Phe Asp Val Asp Lys Glu Thr Lys Gly Tyr Val Gly Tyr Ile Ser 
    370                 375                 380                 


Asn Lys Met Val Leu Thr Asn Pro Gln Lys Gln Lys Thr Ile Gln Gln 
385                 390                 395                 400 


Asn Phe Tyr Asp Tyr Ile Ser Gly Lys Ile Thr Gly Ile Glu Gly Ala 
                405                 410                 415     


Glu Tyr Phe Leu Asn Lys Ile Ser Asp Gly Thr Phe Leu Arg Lys Leu 
            420                 425                 430         


Arg Thr Thr Asp Asn Gly Ala Val Pro Asn Gln Ile His Ala Tyr Glu 
        435                 440                 445             


Leu Glu Lys Ile Ile Asp Arg Gln Gly Glu Asp Tyr Pro Phe Leu Leu 
    450                 455                 460                 


Glu Asn Lys Asp Lys Leu Leu Ser Ile Leu Thr Phe Lys Ile Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ser Lys Gly Asn Asn Ser Arg Phe Gly Trp Ile 
                485                 490                 495     


Lys Arg Thr Ile Ser Gln Asp Ile Leu Asp Asp Asn Asp Glu Asp Thr 
            500                 505                 510         


Arg Asn Gly Lys Ile Arg Pro Trp Asn Tyr Gln Lys Leu Ile Asn Met 
        515                 520                 525             


Asp Glu Thr Arg Asp Ala Phe Ile Thr Asn Leu Ile Gly Asn Asp Ile 
    530                 535                 540                 


Ile Leu Leu Asn Glu Lys Val Leu Pro Lys Arg Ser Leu Ile Tyr Glu 
545                 550                 555                 560 


Glu Val Met Leu Gln Asn Glu Leu Thr Arg Val Lys Tyr Lys Asp Lys 
                565                 570                 575     


Tyr Gly Lys Thr His Phe Phe Asp Ser Glu Leu Arg Gln Glu Ile Ile 
            580                 585                 590         


Asn Asn Leu Phe Lys Thr Asn Asn Lys Arg Val Ser Ser Ala Met Leu 
        595                 600                 605             


Leu Asp Tyr Leu Glu Asn Tyr Thr Asn Leu Gln Ala Val Glu Ile Val 
    610                 615                 620                 


Ser Gly Ile Glu Lys Gly Lys Ser Leu Asn Ser Thr Leu Lys Thr Tyr 
625                 630                 635                 640 


Asn Asp Leu Lys Thr Ile Phe Ser Glu Glu Leu Leu Asp Ser Glu Ile 
                645                 650                 655     


Tyr Gln Lys Glu Leu Glu Glu Ile Val Lys Val Ile Thr Val Phe Asp 
            660                 665                 670         


Asp Lys Lys Ser Ile Lys Asn Tyr Leu Ile Lys Tyr Phe Gly His Leu 
        675                 680                 685             


Glu Phe Leu Asn Glu Glu Arg Ile Asn Gln Leu Ser Lys Leu Arg Tyr 
    690                 695                 700                 


Thr Gly Trp Gly Arg Tyr Ser Ala Lys Leu Leu Leu Asp Ile Arg Asp 
705                 710                 715                 720 


Glu Asp Thr Gly Phe Asn Leu Met Gln Phe Leu Arg Asn Asp Asp Glu 
                725                 730                 735     


Asn Arg Asn Leu Ser Gln Leu Ile Ser Asp Ser Thr Leu Ser Phe Asp 
            740                 745                 750         


Ser Ala Ile Lys Ser Ile Gln Ser Lys Ser Thr Val Glu Asp Asp Val 
        755                 760                 765             


Phe Asp Glu Ile Lys Lys Leu Ala Gly Ser Pro Ala Ile Lys Arg Gly 
    770                 775                 780                 


Ile Leu Asn Ser Ile Lys Ile Val Asp Glu Leu Val Gln Ile Ile Gly 
785                 790                 795                 800 


Tyr Pro Pro Gln Asn Ile Val Ile Glu Met Ala Arg Glu Asn Met Thr 
                805                 810                 815     


Thr Glu Glu Gly Gln Lys Lys Ala Lys Thr Arg Lys Thr Lys Leu Glu 
            820                 825                 830         


Ser Ala Leu Lys Asn Ile Glu Asn Ser Val Leu Glu Asn Gly Lys Val 
        835                 840                 845             


Pro His Ser Asp Glu Gln Leu Gln Ser Glu Lys Leu Tyr Leu Tyr Tyr 
    850                 855                 860                 


Leu Gln Asn Gly Lys Asp Met Tyr Ser Leu Asp Lys Thr Gly Ser Pro 
865                 870                 875                 880 


Thr Pro Leu Tyr Arg Asp Gln Leu Asp Gln Tyr Glu Val Asp His Ile 
                885                 890                 895     


Ile Pro Tyr Ser Phe Leu Pro Ile Asp Ser Ile Asp Asn Lys Val Leu 
            900                 905                 910         


Thr His Arg Glu Asn Asn Gln Arg Lys Leu Asn Asn Ile Pro Asp Lys 
        915                 920                 925             


Glu Thr Val Ala Asn Met Lys Pro Phe Trp Glu Lys Leu Tyr Asn Ala 
    930                 935                 940                 


Lys Leu Ile Ser Gln Thr Lys Tyr Gln Arg Leu Thr Thr Ser Glu Arg 
945                 950                 955                 960 


Thr Pro Asp Gly Val Leu Thr Glu Ser Met Lys Ala Gly Phe Ile Glu 
                965                 970                 975     


Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys His Val Ala Arg Ile 
            980                 985                 990         


Leu Asp Asn Arg Phe Leu Asp Thr  Lys Ile Ile Ala Leu  Lys Ser Gln 
        995                 1000                 1005             


Leu Ile  Thr Asn Phe Arg Asn  Thr Phe His Ile Ala  Lys Ile Arg 
    1010                 1015                 1020             


Glu Leu  Asn Asp Tyr His His  Ala His Asp Ala Tyr  Leu Ala Val 
    1025                 1030                 1035             


Val Val  Gly Gln Thr Leu Leu  Lys Ala Tyr Pro Lys  Leu Ala Pro 
    1040                 1045                 1050             


Glu Leu  Ile Tyr Gly His His  Ala His Phe Asn Arg  His Glu Glu 
    1055                 1060                 1065             


Asn Lys  Ala Thr Ile Arg Lys  His Leu Tyr Ser Asn  Ile Met Arg 
    1070                 1075                 1080             


Phe Phe  Asn Asn Pro Asp Ser  Lys Val Ser Lys Asp  Ile Trp Asp 
    1085                 1090                 1095             


Cys Asn  Arg Asp Leu Pro Ile  Ile Lys Glu Val Ile  Tyr Asn Ser 
    1100                 1105                 1110             


Gln Val  Asn Phe Val Lys Arg  Thr Met Ile Lys Lys  Gly Ala Phe 
    1115                 1120                 1125             


Tyr Asn  Gln Asn Pro Val Gly  Lys Phe Asn Lys Gln  Ile Ala Ala 
    1130                 1135                 1140             


Asn Asn  Arg Tyr Pro Leu Lys  Thr Lys Val Ser Ser  Leu Asp Thr 
    1145                 1150                 1155             


Ser Ile  Tyr Gly Gly Tyr Gly  Pro Met Asn Ser Ala  Leu Ser Ile 
    1160                 1165                 1170             


Ile Ile  Ile Ala Glu Arg Phe  Asn Glu Lys Lys Gly  Lys Ile Glu 
    1175                 1180                 1185             


Thr Val  Lys Glu Phe His Asp  Ile Phe Ile Ile Asp  Tyr Lys Lys 
    1190                 1195                 1200             


Phe Asn  Asn Asn Pro Phe Gln  Phe Leu Asn Asp Thr  Ser Glu Asn 
    1205                 1210                 1215             


Gly Phe  Leu Lys Lys Asn Asn  Ile Asn Arg Val Leu  Ala Phe Tyr 
    1220                 1225                 1230             


Gly Val  Pro Lys Tyr Ser Leu  Met Gln Lys Asn Asp  Gly Thr Arg 
    1235                 1240                 1245             


Met Leu  Phe Glu Ser Lys Ser  Asn Leu His Lys Ala  Thr Gln Phe 
    1250                 1255                 1260             


Lys Leu  Thr Lys Thr Gln Asn  Glu Leu Phe Phe His  Met Arg Arg 
    1265                 1270                 1275             


Leu Leu  Thr Lys Ser Asn Leu  Met Asp Leu Lys Ser  Glu Ser Ala 
    1280                 1285                 1290             


Ile Lys  Glu Ser Gln Asn Phe  Ile Leu Lys His Lys  Glu Glu Phe 
    1295                 1300                 1305             


Asp Asn  Ile Ser Asn Gln Leu  Ser Ala Phe Ser Gln  Lys Met Leu 
    1310                 1315                 1320             


Gly Asp  Thr Thr Ser Leu Arg  Asn Leu Ile Lys Gly  Tyr Asn Glu 
    1325                 1330                 1335             


Arg Lys  Ile Lys Glu Ile Asp  Ser Ser Glu Glu Val  Ile Lys Tyr 
    1340                 1345                 1350             


Phe Tyr  Asp Asn Phe Ile Lys  Met Phe Ser Phe Val  Lys Ser Gly 
    1355                 1360                 1365             


Ala Pro  Lys Asp Ile Asn Asp  Phe Phe Asn Asn Lys  Tyr Thr Val 
    1370                 1375                 1380             


Ala Arg  Met Arg Pro Lys Pro  Asp Lys Lys Phe Leu  Asn Ala Thr 
    1385                 1390                 1395             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1400                 1405                 1410             


Leu Ser  Lys Leu Gly Glu Asp  
    1415                 1420 


<210>  1212
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1212

Met Ala Lys Phe Asn Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Val 
1               5                   10                  15      


Ser Ser Val Gly Tyr Ala Val Val Thr Glu Asp Tyr Arg Val Pro Ala 
            20                  25                  30          


Phe Lys Phe Lys Val Leu Gly Asn Thr Glu Lys Glu Lys Ile Lys Lys 
        35                  40                  45              


Asn Leu Ile Gly Ser Thr Thr Phe Val Pro Ala Gln Ser Ala Gln Gly 
    50                  55                  60                  


Thr Arg Val Phe Arg Val Asn Arg Arg Arg Ile Asp Arg Arg Asn His 
65                  70                  75                  80  


Arg Ile Ala Tyr Leu Arg Asp Ile Phe Gln Lys Glu Ile Glu Lys Ile 
                85                  90                  95      


Asp Lys Asn Phe Tyr Arg Arg Leu Asp Glu Ser Phe Arg Val Leu Gly 
            100                 105                 110         


Asp Lys Ser Glu Asp Ile Gln Ile Lys Gln Pro Phe Phe Gly Asn Lys 
        115                 120                 125             


Glu Leu Glu Thr Ala Tyr His Lys Lys Tyr Pro Thr Ile Tyr His Leu 
    130                 135                 140                 


Arg Lys His Leu Ala Asp Ala Asp Lys Asn Ser Pro Val Ala Asp Ile 
145                 150                 155                 160 


Arg Glu Val Tyr Met Val Leu Ser His Ile Leu Lys Tyr Arg Gly His 
                165                 170                 175     


Phe Leu Thr Leu Gly Lys Ile Asp Pro Asn Asn Ile Asn Met Gln Asn 
            180                 185                 190         


Ser Trp Ile Asp Phe Ile Glu Ser Cys Gln Asp Ala Phe Asp Leu Glu 
        195                 200                 205             


Ile Ser Asp Glu Ser Lys Thr Ile Ala Ala Ile Phe Lys Ser Ser Glu 
    210                 215                 220                 


Asn Arg Gln Glu Lys Val Lys Gln Ile Leu Ser Tyr Phe Gln Gln Glu 
225                 230                 235                 240 


Leu Leu Lys Lys Asp Lys Ser Ile Phe Lys Gln Leu Leu Gln Leu Leu 
                245                 250                 255     


Phe Gly Leu Lys Thr Lys Phe Lys Asp Cys Phe Glu Leu Glu Glu Glu 
            260                 265                 270         


Pro Asp Leu Asn Phe Ser Lys Glu Asn Tyr Asp Glu Asn Leu Glu Asn 
        275                 280                 285             


Leu Leu Gly Ala Leu Glu Glu Asp Phe Ser Asp Val Phe Ala Lys Leu 
    290                 295                 300                 


Lys Ile Leu Arg Asp Thr Ile Leu Leu Ser Ser Met Leu Thr Tyr Thr 
305                 310                 315                 320 


Gly Ala Thr His Ala Arg Phe Ser Ala Thr Met Val Glu Arg Tyr Glu 
                325                 330                 335     


Glu His Arg Lys Asp Leu Gln Arg Phe Lys Ser Phe Val Lys Gln Asn 
            340                 345                 350         


Leu Ser Glu Gln Asp Tyr Leu Asp Ile Phe Gly Arg Lys Thr Pro Thr 
        355                 360                 365             


Gly Phe Asp Val Asp Lys Glu Thr Lys Gly Tyr Val Gly Tyr Ile Ser 
    370                 375                 380                 


Asn Lys Met Val Leu Thr Asn Pro Gln Lys Gln Lys Thr Ile Gln Gln 
385                 390                 395                 400 


Asn Phe Tyr Asp Tyr Ile Ser Gly Lys Ile Thr Gly Ile Glu Gly Ala 
                405                 410                 415     


Glu Cys Phe Leu Asn Lys Ile Ser Asp Gly Thr Phe Leu Arg Lys Leu 
            420                 425                 430         


Arg Thr Thr Asp Asn Gly Ala Ile Pro Asn Gln Ile His Ala Tyr Glu 
        435                 440                 445             


Leu Glu Lys Ile Ile Glu Arg Gln Gly Arg Asp Tyr Pro Phe Leu Leu 
    450                 455                 460                 


Glu Asn Lys Asp Lys Leu Leu Ser Ile Leu Thr Phe Lys Ile Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ala Lys Gly Asn Asn Ser Arg Phe Ala Trp Ile 
                485                 490                 495     


Lys Arg Thr Thr Ser Gln Asp Val Leu Asp Asp Asn Asp Glu Asp Thr 
            500                 505                 510         


Arg Asn Gly Lys Ile Arg Pro Trp Asn Tyr His Lys Leu Ile Asn Met 
        515                 520                 525             


Asp Glu Thr Arg Asp Ala Phe Ile Ile Asn Leu Ile Gly Asn Asp Ile 
    530                 535                 540                 


Ile Leu Leu Asn Glu Lys Val Leu Pro Lys Arg Ser Leu Ile Tyr Glu 
545                 550                 555                 560 


Glu Val Met Leu Gln Asn Glu Leu Thr Arg Val Lys Tyr Lys Asp Lys 
                565                 570                 575     


Tyr Gly Lys Ile His Phe Phe Asn Ser Glu Leu Arg Gln Glu Ile Ile 
            580                 585                 590         


Asn Asn Leu Phe Lys Thr Asn Ser Lys Arg Val Ser Ser Ala Met Leu 
        595                 600                 605             


Leu Ser Tyr Leu Glu Asn Phe Thr Asn Leu Gln Ala Val Glu Ile Val 
    610                 615                 620                 


Ser Gly Ile Glu Lys Gly Lys Ser Leu Asn Ser Thr Leu Lys Thr Tyr 
625                 630                 635                 640 


Asn Asp Leu Lys Thr Ile Phe Ser Glu Glu Leu Leu Asp Ser Glu Ile 
                645                 650                 655     


Tyr Gln Lys Glu Leu Glu Glu Ile Ile Lys Val Ile Thr Val Phe Asp 
            660                 665                 670         


Asp Lys Lys Ser Ile Lys Asn Tyr Leu Thr Lys Tyr Phe Gly His Leu 
        675                 680                 685             


Glu Phe Leu Asn Glu Glu Lys Ile Asn Gln Leu Ser Lys Leu Arg Tyr 
    690                 695                 700                 


Thr Gly Trp Gly Arg Tyr Ser Gly Lys Leu Leu Leu Gly Ile Arg Asp 
705                 710                 715                 720 


Glu Asp Thr Gly Phe Asn Leu Met Gln Phe Leu Arg Asn Asp Asp Glu 
                725                 730                 735     


Asn Arg Asn Leu Thr Gln Leu Ile Ser Asp Thr Thr Leu Ser Phe Asp 
            740                 745                 750         


Ser Ala Ile Lys Ala Ile Gln Ser Lys Ser Thr Val Glu Asp Asp Val 
        755                 760                 765             


Phe Glu Glu Ile Lys Asn Leu Ala Gly Ser Pro Ala Ile Lys Arg Gly 
    770                 775                 780                 


Ile Leu Asn Ser Ile Lys Ile Val Asp Glu Leu Val Gln Ile Ile Gly 
785                 790                 795                 800 


Tyr Pro Pro Gln Asn Ile Ile Ile Glu Met Ala Arg Glu Asn Met Thr 
                805                 810                 815     


Thr Glu Glu Gly Gln Lys Lys Ala Lys Thr Arg Lys Ser Lys Leu Glu 
            820                 825                 830         


Ser Ala Leu Lys Asn Ile Glu Asp Ser Val Leu Glu Ser Gly Lys Val 
        835                 840                 845             


Pro His Ser Asp Glu Gln Leu Gln Ser Glu Lys Leu Tyr Leu Tyr Tyr 
    850                 855                 860                 


Leu Gln Asn Gly Lys Asp Met Tyr Ser Leu Asp Lys Thr Gly Ser Pro 
865                 870                 875                 880 


Thr Pro Leu Tyr Arg Asp Gln Leu Asp Gln Tyr Glu Val Asp His Ile 
                885                 890                 895     


Ile Pro Tyr Ser Phe Leu Pro Ile Asp Ser Ile Asp Asn Lys Val Leu 
            900                 905                 910         


Thr His Arg Glu Asn Asn Gln Arg Lys Leu Asn Asn Ile Pro Asp Lys 
        915                 920                 925             


Glu Thr Val Ala Asn Met Lys Pro Phe Trp Glu Lys Leu Tyr Asn Ala 
    930                 935                 940                 


Lys Leu Ile Ser Gln Thr Lys Tyr Gln Arg Leu Thr Thr Ser Glu Arg 
945                 950                 955                 960 


Thr Pro Asp Gly Val Leu Thr Glu Ser Met Lys Ala Gly Phe Ile Glu 
                965                 970                 975     


Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys His Val Ala Arg Ile 
            980                 985                 990         


Leu Asp Asn Arg Phe Ser Asp Thr  Lys Ile Ile Thr Leu  Lys Ser Gln 
        995                 1000                 1005             


Leu Val  Thr Asn Phe Arg Asn  Thr Phe His Ile Ala  Lys Ile Arg 
    1010                 1015                 1020             


Glu Leu  Asn Asp Tyr His His  Ala His Asp Ala Tyr  Leu Ala Val 
    1025                 1030                 1035             


Val Val  Gly Gln Thr Leu Leu  Lys Ala Tyr Pro Lys  Leu Ala Pro 
    1040                 1045                 1050             


Glu Leu  Ile Tyr Gly His His  Ala His Phe Asn Arg  His Glu Glu 
    1055                 1060                 1065             


Asn Lys  Ala Thr Leu Arg Lys  His Leu Tyr Ser Asn  Ile Met Arg 
    1070                 1075                 1080             


Phe Phe  Asn Asn Pro Asp Ser  Lys Val Ser Lys Asp  Ile Trp Asp 
    1085                 1090                 1095             


Cys Asn  Arg Asp Leu Pro Ile  Val Lys Asp Val Ile  Tyr Asn Ser 
    1100                 1105                 1110             


Gln Ile  Asn Phe Val Lys Arg  Thr Met Ile Lys Lys  Gly Ala Phe 
    1115                 1120                 1125             


Tyr Asn  Gln Asn Pro Val Gly  Lys Leu Asn Lys Gln  Ile Ala Ala 
    1130                 1135                 1140             


Asn Asn  Arg Tyr Pro Leu Lys  Thr Lys Val Ser Ser  Leu Asp Thr 
    1145                 1150                 1155             


Ser Ile  Tyr Gly Gly Tyr Gly  Pro Met Asn Ser Ala  Leu Ser Ile 
    1160                 1165                 1170             


Ile Ile  Ile Ala Glu Arg Phe  Asn Glu Lys Lys Gly  Lys Ile Glu 
    1175                 1180                 1185             


Thr Val  Lys Glu Phe His Asp  Ile Phe Ile Ile Asp  Tyr Glu Lys 
    1190                 1195                 1200             


Phe Asn  Asn Asn Pro Phe Gln  Phe Leu Asn Asn Val  Ser Glu Glu 
    1205                 1210                 1215             


Gly Phe  Leu Lys Lys Asn Asp  Leu Asn Arg Val Leu  Ala Phe Tyr 
    1220                 1225                 1230             


Arg Ile  Ser Lys Tyr Ser Leu  Met Gln Lys Ile Asp  Gly Thr Arg 
    1235                 1240                 1245             


Met Leu  Phe Glu Ser Lys Ser  Asn Leu His Lys Val  Thr Gln Phe 
    1250                 1255                 1260             


Lys Leu  Thr Lys Thr Gln Asn  Glu Leu Phe Phe His  Met Lys Arg 
    1265                 1270                 1275             


Leu Leu  Thr Lys Ser Asn Leu  Met Asp Leu Lys Ser  Glu Ser Ala 
    1280                 1285                 1290             


Ile Lys  Glu Ser Gln Asn Phe  Ile Gln Thr His Gln  Asn Glu Phe 
    1295                 1300                 1305             


Asp Asn  Ile Ala Asp Arg Leu  Ser Asn Tyr Ser Gln  Lys Met Leu 
    1310                 1315                 1320             


Gly Glu  Thr Lys Ser Leu Gln  Thr Leu Ile Asn Gly  Tyr Asn Glu 
    1325                 1330                 1335             


Arg Pro  Ile Lys Gln Ile Asp  Asn Asn Glu Glu Thr  Ile Arg Tyr 
    1340                 1345                 1350             


Tyr Tyr  Asp Asn Phe Ile Lys  Met Phe Ser Phe Val  Lys Ser Gly 
    1355                 1360                 1365             


Ala Pro  Lys Asp Ile Ile Asn  Phe Phe Asn Asp Lys  Tyr Thr Val 
    1370                 1375                 1380             


Pro Arg  Met Arg Pro Lys Pro  Asp Arg Lys Met Leu  Asp Ala Thr 
    1385                 1390                 1395             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1400                 1405                 1410             


Leu Ser  Lys Leu Gly Glu Asp  
    1415                 1420 


<210>  1213
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1213

Met Ser Arg Lys Asn Tyr Val Asp Asp Tyr Ala Ile Ser Leu Asp Ile 
1               5                   10                  15      


Gly Asn Ala Ser Val Gly Trp Ser Ala Phe Thr Pro Asn Tyr Arg Leu 
            20                  25                  30          


Val Arg Ala Lys Gly His Glu Leu Ile Gly Val Arg Leu Phe Asp Pro 
        35                  40                  45              


Ala Asp Thr Ala Glu Ser Arg Arg Met Ala Arg Thr Thr Arg Arg Arg 
    50                  55                  60                  


Tyr Ser Arg Arg Arg Trp Arg Leu Arg Leu Leu Asp Ala Leu Phe Asp 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Ile Asp Pro Ser Phe Leu Ala Arg Arg Lys Tyr 
                85                  90                  95      


Ser Trp Val His Pro Asp Asp Glu Asn Asn Ala Asp Cys Trp Tyr Gly 
            100                 105                 110         


Ser Val Leu Phe Asp Ser Asn Glu Gln Asp Lys Arg Phe Tyr Glu Lys 
        115                 120                 125             


Tyr Pro Thr Ile Tyr His Leu Arg Lys Ala Leu Met Glu Asp Asp Ser 
    130                 135                 140                 


Gln His Asp Ile Arg Glu Ile Tyr Leu Ala Ile His His Met Val Lys 
145                 150                 155                 160 


Tyr Arg Gly Asn Phe Leu Val Glu Gly Thr Leu Glu Ser Ser Asn Ala 
                165                 170                 175     


Phe Lys Glu Asp Glu Leu Leu Lys Leu Leu Gly Arg Ile Thr Arg Tyr 
            180                 185                 190         


Glu Met Ser Glu Gly Glu Gln Asn Ser Asp Ile Glu Gln Asp Asp Glu 
        195                 200                 205             


Asn Lys Leu Val Ala Pro Ala Asn Gly Gln Leu Ala Asp Ala Leu Cys 
    210                 215                 220                 


Ala Thr Arg Gly Ser Arg Ser Met Arg Val Asp Asn Ala Leu Glu Ala 
225                 230                 235                 240 


Leu Ser Ala Val Asn Asp Leu Ser Arg Glu Gln Arg Ala Ile Val Lys 
                245                 250                 255     


Ala Ile Phe Ala Gly Leu Glu Gly Asn Lys Leu Asp Leu Ala Lys Ile 
            260                 265                 270         


Phe Val Ser Lys Glu Phe Ser Ser Glu Asn Lys Lys Ile Leu Gly Ile 
        275                 280                 285             


Tyr Phe Asn Lys Ser Asp Tyr Glu Glu Lys Cys Val Gln Ile Val Asp 
    290                 295                 300                 


Ser Gly Leu Leu Asp Asp Glu Glu Arg Glu Phe Leu Asp Arg Met Gln 
305                 310                 315                 320 


Gly Gln Tyr Asn Ala Ile Ala Leu Lys Gln Leu Leu Gly Arg Ser Thr 
                325                 330                 335     


Ser Val Ser Asp Ser Lys Cys Ala Ser Tyr Asp Ala His Arg Ala Asn 
            340                 345                 350         


Trp Asn Leu Ile Lys Leu Gln Leu Arg Thr Lys Glu Asn Glu Lys Asp 
        355                 360                 365             


Ile Asn Glu Asn Tyr Gly Ile Leu Val Gly Trp Lys Ile Asp Ser Gly 
    370                 375                 380                 


Gln Arg Lys Ser Val Arg Gly Glu Ser Ala Tyr Glu Asn Met Arg Lys 
385                 390                 395                 400 


Lys Ala Asn Val Phe Phe Lys Lys Met Ile Glu Thr Ser Asp Leu Ser 
                405                 410                 415     


Glu Thr Asp Lys Asn Arg Leu Ile His Asp Ile Glu Glu Asp Lys Leu 
            420                 425                 430         


Phe Pro Ile Gln Arg Asp Ser Asp Asn Gly Val Ile Pro His Gln Leu 
        435                 440                 445             


His Gln Asn Glu Leu Lys Gln Ile Ile Lys Lys Gln Gly Lys Tyr Tyr 
    450                 455                 460                 


Pro Phe Leu Leu Asp Ala Phe Glu Lys Asp Gly Lys Gln Ile Asn Lys 
465                 470                 475                 480 


Ile Glu Gly Leu Leu Thr Phe Arg Val Pro Tyr Phe Val Gly Pro Leu 
                485                 490                 495     


Val Val Pro Glu Asp Leu Gln Lys Ser Asp Asn Ser Glu Asn His Trp 
            500                 505                 510         


Met Val Arg Lys Lys Lys Gly Glu Ile Thr Pro Trp Asn Phe Asp Glu 
        515                 520                 525             


Met Val Asp Lys Asp Ala Ser Gly Arg Lys Phe Ile Glu Arg Leu Val 
    530                 535                 540                 


Gly Thr Asp Ser Tyr Leu Leu Gly Glu Pro Thr Leu Pro Lys Asn Ser 
545                 550                 555                 560 


Leu Leu Tyr Gln Glu Tyr Glu Val Leu Asn Glu Leu Asn Asn Val Arg 
                565                 570                 575     


Leu Ser Val Arg Thr Gly Asn His Trp Asn Asp Lys Arg Arg Met Arg 
            580                 585                 590         


Leu Gly Arg Glu Glu Lys Thr Leu Leu Cys Gln Arg Leu Phe Met Lys 
        595                 600                 605             


Gly Gln Thr Val Thr Lys Arg Thr Ala Glu Asn Leu Leu Arg Lys Glu 
    610                 615                 620                 


Tyr Gly Arg Thr Tyr Glu Leu Ser Gly Leu Ser Asp Glu Ser Lys Phe 
625                 630                 635                 640 


Thr Ser Ser Leu Ser Thr Tyr Gly Lys Met Cys Arg Ile Phe Gly Glu 
                645                 650                 655     


Lys Tyr Val Asn Glu His Arg Asp Leu Met Glu Lys Ile Val Glu Leu 
            660                 665                 670         


Gln Thr Val Phe Glu Asp Lys Glu Thr Leu Leu His Gln Leu Arg Gln 
        675                 680                 685             


Leu Glu Gly Ile Ser Glu Ala Asp Cys Ala Leu Leu Val Asn Thr His 
    690                 695                 700                 


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Thr Lys Ala 
705                 710                 715                 720 


Gly Glu Cys Lys Ile Ser Asp Asp Phe Ala Pro Arg Lys His Ser Ile 
                725                 730                 735     


Ile Glu Ile Met Arg Ala Glu Asp Arg Asn Leu Met Glu Ile Ile Thr 
            740                 745                 750         


Asp Lys Gln Leu Gly Phe Ser Asp Trp Ile Glu Gln Glu Asn Leu Gly 
        755                 760                 765             


Ala Glu Asn Gly Ser Ser Leu Met Glu Val Val Asp Asp Leu Arg Val 
    770                 775                 780                 


Ser Pro Lys Val Lys Arg Gly Ile Ile Gln Ser Ile Arg Leu Ile Asp 
785                 790                 795                 800 


Asp Ile Ser Lys Ala Val Gly Lys Arg Pro Ser Arg Ile Phe Leu Glu 
                805                 810                 815     


Leu Ala Asp Asp Ile Gln Pro Ser Gly Arg Thr Ile Ser Arg Lys Ser 
            820                 825                 830         


Arg Leu Gln Asp Leu Tyr Arg Asn Ala Asn Leu Gly Lys Glu Phe Lys 
        835                 840                 845             


Gly Ile Ala Asp Glu Leu Asn Ala Cys Ser Asp Lys Asp Leu Gln Asp 
    850                 855                 860                 


Asp Arg Leu Phe Leu Tyr Tyr Thr Gln Leu Gly Lys Asp Met Tyr Thr 
865                 870                 875                 880 


Gly Glu Glu Leu Asp Leu Asp Arg Leu Ser Ser Ala Tyr Asp Ile Asp 
                885                 890                 895     


His Ile Ile Pro Gln Ala Val Thr Gln Asn Asp Ser Ile Asp Asn Arg 
            900                 905                 910         


Val Leu Val Ala Arg Ala Glu Asn Ala Arg Lys Thr Asp Ser Phe Thr 
        915                 920                 925             


Tyr Met Pro Gln Ile Ala Asp Arg Met Arg Asn Phe Trp Gln Ile Leu 
    930                 935                 940                 


Leu Asp Asn Gly Leu Ile Ser Arg Val Lys Phe Glu Arg Leu Thr Arg 
945                 950                 955                 960 


Gln Asn Glu Phe Ser Glu Arg Glu Lys Glu Arg Phe Val Gln Arg Ser 
                965                 970                 975     


Leu Val Glu Thr Arg Gln Ile Met Lys Asn Val Ala Thr Leu Met Arg 
            980                 985                 990         


Gln Arg Tyr Gly Asn Ser Ala Ala  Val Ile Gly Leu Asn  Ala Glu Leu 
        995                 1000                 1005             


Thr Lys  Glu Met His Arg Tyr  Leu Gly Phe Ser His  Lys Asn Arg 
    1010                 1015                 1020             


Asp Ile  Asn Asp Tyr His His  Ala Gln Asp Ala Leu  Cys Val Gly 
    1025                 1030                 1035             


Ile Ala  Gly Gln Phe Ala Ala  Asn Arg Gly Phe Phe  Ala Asp Gly 
    1040                 1045                 1050             


Glu Val  Ser Asp Gly Ala Gln  Asn Ser Tyr Asn Gln  Tyr Leu Arg 
    1055                 1060                 1065             


Asp Tyr  Leu Arg Gly Tyr Arg  Glu Lys Leu Ser Ala  Glu Asp Arg 
    1070                 1075                 1080             


Lys Gln  Gly Arg Ala Phe Gly  Phe Ile Val Gly Ser  Met Arg Ser 
    1085                 1090                 1095             


Gln Asp  Glu Gln Lys Arg Val  Asn Pro Arg Thr Gly  Glu Val Val 
    1100                 1105                 1110             


Trp Ser  Glu Glu Asp Lys Asp  Tyr Leu Arg Lys Val  Met Asn Tyr 
    1115                 1120                 1125             


Arg Lys  Met Leu Val Thr Gln  Lys Val Gly Asp Asp  Phe Gly Ala 
    1130                 1135                 1140             


Leu Tyr  Asp Glu Thr Arg Tyr  Ala Ala Thr Asp Pro  Lys Gly Ile 
    1145                 1150                 1155             


Lys Gly  Ile Pro Phe Asp Gly  Ala Lys Gln Asp Thr  Ser Leu Tyr 
    1160                 1165                 1170             


Gly Gly  Phe Ser Ser Ala Lys  Pro Ala Tyr Ala Val  Leu Ile Glu 
    1175                 1180                 1185             


Ser Lys  Gly Lys Thr Arg Leu  Val Asn Val Thr Met  Gln Glu Tyr 
    1190                 1195                 1200             


Ser Leu  Leu Gly Asp Arg Pro  Ser Asp Asp Glu Leu  Arg Lys Val 
    1205                 1210                 1215             


Leu Ala  Lys Lys Lys Ser Glu  Tyr Ala Lys Ala Asn  Ile Leu Leu 
    1220                 1225                 1230             


Arg His  Val Pro Lys Met Gln  Leu Ile Arg Tyr Gly  Gly Gly Leu 
    1235                 1240                 1245             


Met Val  Ile Lys Ser Ala Gly  Glu Leu Asn Asn Ala  Gln Gln Leu 
    1250                 1255                 1260             


Trp Leu  Pro Tyr Glu Glu Tyr  Cys Tyr Phe Asp Asp  Leu Ser Gln 
    1265                 1270                 1275             


Gly Lys  Gly Ser Leu Glu Lys  Asp Asp Leu Lys Lys  Leu Leu Asp 
    1280                 1285                 1290             


Ser Ile  Leu Gly Ser Val Gln  Cys Leu Tyr Pro Trp  His Arg Phe 
    1295                 1300                 1305             


Thr Glu  Glu Glu Leu Ala Asp  Leu His Val Ala Phe  Asp Lys Leu 
    1310                 1315                 1320             


Pro Glu  Asp Glu Lys Lys Asn  Val Ile Thr Gly Ile  Val Ser Ala 
    1325                 1330                 1335             


Leu His  Ala Asp Ala Lys Thr  Ala Asn Leu Ser Ile  Val Gly Met 
    1340                 1345                 1350             


Thr Gly  Ser Trp Arg Arg Met  Asn Asn Lys Ser Gly  Tyr Thr Phe 
    1355                 1360                 1365             


Ser Asp  Glu Asp Glu Phe Ile  Phe Gln Ser Pro Ser  Gly Leu Phe 
    1370                 1375                 1380             


Glu Lys  Arg Val Thr Val Gly  Glu Leu Lys Arg Lys  Ala Lys Lys 
    1385                 1390                 1395             


Glu Val  Asn Ser Lys Tyr Arg  Thr Asn Glu Lys Arg  Leu Pro Thr 
    1400                 1405                 1410             


Leu Ser  Gly Ala Ser Gln Pro  
    1415                 1420 


<210>  1214
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1214

Met Ala Lys Pro Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Val Val Thr Asp Asp Gln Asn Asn Ile Leu Arg Ile 
            20                  25                  30          


Lys Gly Lys Lys Ala Ile Gly Ala Arg Leu Phe Ala Glu Gly Lys Val 
        35                  40                  45              


Ala Ala Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Ile Ser Arg 
    50                  55                  60                  


Arg Arg Trp Arg Ile Lys Leu Leu Glu Glu Leu Phe Asp Glu Glu Ile 
65                  70                  75                  80  


Ala Lys Val Asp Pro Ser Phe Phe Ala Arg Leu His Glu Ser Trp Ile 
                85                  90                  95      


Ser Pro Lys Asp Lys Arg Lys Arg Tyr Ser Ala Ile Val Phe Pro Ser 
            100                 105                 110         


Pro Glu Glu Asp Lys Lys Phe His Glu Ser Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Asp Lys Leu Met Lys Asp Asp Gln Lys His Asp Ile Arg Glu 
    130                 135                 140                 


Ile Tyr Ile Ala Val His Gln Met Ile Lys Val Arg Gly Asn Phe Leu 
145                 150                 155                 160 


His Asp Glu Ser Val Glu Thr Tyr Arg Ser Gly Met Ser Ser Leu Gly 
                165                 170                 175     


Gly Arg Ser Glu Arg Thr Ile Leu Ser Val Gln Thr Leu Glu Glu Leu 
            180                 185                 190         


Asn Asp Leu Phe Ala Glu Asn Glu Gly Thr Glu Glu Val Glu Leu Asn 
        195                 200                 205             


Val Ala Ser Ala Glu Gln Ile Asn Asp Ile Leu Thr Gly Asp His Leu 
    210                 215                 220                 


Asn Ala Asp Ser Gln Lys Glu Ile Ser Asn Leu Leu Leu Pro Ser Ser 
225                 230                 235                 240 


Phe Pro Ser Phe Asp Asp Lys Ala Lys Glu Lys Gln Val Lys Lys Leu 
                245                 250                 255     


Ile Asn Asn Val Ala Thr Asn Ile Ser Lys Ala Trp Leu Gly Tyr Lys 
            260                 265                 270         


Ala Asp Phe Ser Thr Ile Leu Asn Leu Ala Lys Val Asp Lys Asp Gln 
        275                 280                 285             


Lys Lys Ile Phe Ala Phe Ala Leu Gln Gly Gly Asp Glu Glu Asp Lys 
    290                 295                 300                 


Val Gln Glu Leu Glu Ser Leu Leu Glu Gln Ser Gln Thr Asp Ile Val 
305                 310                 315                 320 


Asp Arg Leu Ile Glu Ile Arg His Ala Ile Val Leu Ser Glu Ile Val 
                325                 330                 335     


Pro Val Gly Met Thr Leu Ser Glu Ala Met Ile Asp Lys Tyr Asp Gln 
            340                 345                 350         


His Lys Glu Asp Leu Ile Thr Leu Lys Ala Val Ile Arg Asn Thr Lys 
        355                 360                 365             


Asp Lys Lys Lys Ala Ala Lys Leu Gln Ala Ile Tyr Asp Leu Tyr Val 
    370                 375                 380                 


Asn Asn Arg His Ala Asp Leu Ala Lys Ala Met Lys Leu Thr Gly Ile 
385                 390                 395                 400 


Lys Lys Arg Ser Glu Leu Leu Asp Pro Glu Glu Leu Lys Lys Ala Ile 
                405                 410                 415     


Ser Ser Leu Leu Asp Asp Ser Pro Glu Ala Gly Glu Ile Lys Gln Arg 
            420                 425                 430         


Leu Glu Gly Lys Thr Phe Leu Pro Leu Gln Arg Ser Asn Asn Asn Gly 
        435                 440                 445             


Val Ile Pro Asn Gln Leu His Gln Val Glu Leu Asp Glu Ile Ile Lys 
    450                 455                 460                 


Lys Gln Ser Lys Tyr Tyr Pro Phe Leu Ala Glu Lys Asn Pro Asp Glu 
465                 470                 475                 480 


Ser Glu Glu Ala Gln Lys Lys Ala Pro Thr Lys Leu Asp Ala Leu Leu 
                485                 490                 495     


Thr Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Lys Glu Glu 
            500                 505                 510         


Gln Glu Ala Gln Asp Gly His Ser Phe Ala Trp Met Val Arg Arg Asp 
        515                 520                 525             


Pro Gln Asp His Glu Ala Ile Thr Pro Trp Asn Phe Glu Lys Lys Val 
    530                 535                 540                 


Asp Lys Met Ala Ser Ala Thr Gln Phe Ile Lys Arg Met Thr Thr Lys 
545                 550                 555                 560 


Asp Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Ala Ser Ser Leu Lys 
                565                 570                 575     


Tyr Gln Leu Phe Thr Val Leu Asn Glu Leu Asn Asn Leu Arg Val Asn 
            580                 585                 590         


Gly Lys Lys Leu Thr Ser Asp Glu Lys Glu Gln Val Ile Glu Gly Leu 
        595                 600                 605             


Phe Lys Lys Gln Lys Thr Val Lys Ala Asp Lys Phe Val Lys Tyr Trp 
    610                 615                 620                 


Gln Ala Ile His Ile Gly Ala Asp Ile Lys Val Lys Gly Leu Ser Asp 
625                 630                 635                 640 


Pro Ser Lys Phe Asn Ser Thr Met Ser Thr Tyr Ile Asp Phe Lys Lys 
                645                 650                 655     


Ile Phe Gly Asp Gln Leu Asn Asp Ala Asn Arg Gln Asn Asp Phe Glu 
            660                 665                 670         


Lys Ile Ile Glu Tyr Ser Thr Ile Phe Glu Asp Arg Lys Ile Leu Glu 
        675                 680                 685             


Asp Lys Leu Arg Ala Asp Phe Ala Trp Leu Ser Glu Glu Gln Ile Lys 
    690                 695                 700                 


Gln Leu Cys Arg Leu Arg Leu Gln Gly Trp Gly Arg Leu Ser Asp Lys 
705                 710                 715                 720 


Leu Leu Thr Arg Leu Thr Asp Ala Asp Gly Gln Asn Val Leu Glu Arg 
                725                 730                 735     


Leu Trp Asn Ser Asn Asp Asn Phe Val Gln Ile Val Ser Asp Pro Asn 
            740                 745                 750         


Ile Lys Ala Lys Ile Glu Gln Glu Asn Gln Lys Leu Leu Arg Asp Gly 
        755                 760                 765             


Gln Ser Asp Asp Ala Val Glu Ser Ile Leu Asp Asp Ala Tyr Thr Ser 
    770                 775                 780                 


Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Met Arg Val Val His Asp 
785                 790                 795                 800 


Ile Val Lys Ala Cys Asp Gly Asn Val Pro Ala Lys Phe Ala Ile Glu 
                805                 810                 815     


Phe Ala Arg Ser Gln Glu Asp Asp Pro Lys Arg Thr Thr Ala Arg Val 
            820                 825                 830         


Asn Gln Met Gln Lys Ile Tyr Asp Gln Ile Ser Asp Glu Val Val Ser 
        835                 840                 845             


Gln Gly Val Lys Glu Gln Leu Ser Gly Met Lys Ser Leu Lys Asp Arg 
    850                 855                 860                 


Tyr Tyr Leu Tyr Phe Met Gln Gly Gly Arg Asp Ala Tyr Thr Gly Ala 
865                 870                 875                 880 


Lys Leu Asn Ile Asp Arg Leu Ser Asp Tyr Asp Ile Asp His Ile Met 
                885                 890                 895     


Pro Gln Ser Phe Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val 
            900                 905                 910         


Ala Arg Ala Val Asn Asn Gln Lys Ser Asp Lys Val Pro Ala Phe Leu 
        915                 920                 925             


Phe Gly Asn Lys Val Val Ala Asp Leu Gly Ile Thr Val Arg Glu Met 
    930                 935                 940                 


Trp Asp Lys Trp Gln Lys Leu Gly Met Ile Ser Lys Arg Lys Leu Ser 
945                 950                 955                 960 


Asn Leu Leu Thr Asp Pro Asp Ala Leu Thr Glu Tyr Arg Ala Gln Gly 
                965                 970                 975     


Phe Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Thr 
            980                 985                 990         


Ala Thr Ile Leu Gln Ser Glu Phe  Pro Asp Ser Lys Ile  Ile Glu Val 
        995                 1000                 1005             


Pro Ala  Lys Tyr Asn Ser Ile  Val Arg Lys Gln Phe  Asp Leu Tyr 
    1010                 1015                 1020             


Lys Ser  Arg Glu Val Asn Asp  Phe His His Ala Ile  Asp Ala Tyr 
    1025                 1030                 1035             


Leu Ser  Thr Ile Val Gly Asn  Tyr Leu Tyr Gln Val  Tyr Pro Asn 
    1040                 1045                 1050             


Leu Arg  Arg Met Phe Val Tyr  Gly Glu Phe Lys Lys  Phe Ser Ser 
    1055                 1060                 1065             


Asn Ala  Glu Glu Ser Ala His  Asp Met Ala Arg Arg  Val Lys Ser 
    1070                 1075                 1080             


Met Asn  Phe Leu Asp Asp Leu  Leu Arg Gly Thr His  Gly Asp Asn 
    1085                 1090                 1095             


Ile Tyr  Cys Arg Ser Thr Gly  Glu Ile Val Phe Asn  Arg Asn Asp 
    1100                 1105                 1110             


Ile Ile  Ser Lys Leu Lys Gln  Ala Tyr Ser Phe Lys  Gln Met Leu 
    1115                 1120                 1125             


Val Thr  Gln Glu Val Phe Thr  Arg Lys Ser Ala Leu  Phe Asp Gln 
    1130                 1135                 1140             


Thr Val  Tyr Pro Ser Pro Glu  Arg Asp Ser Lys Lys  Arg Ser Gly 
    1145                 1150                 1155             


Leu Ile  Pro Arg Lys Lys Gly  Met Asp Thr Glu Ile  Tyr Gly Gly 
    1160                 1165                 1170             


Tyr Ser  Gly Asn Lys Asp Ala  Tyr Phe Val Leu Ala  Glu Ala Val 
    1175                 1180                 1185             


Lys Glu  Lys Gly His Thr Leu  Gln Ile Val Gly Val  Pro Ile Arg 
    1190                 1195                 1200             


Ala Leu  Asn Thr Leu Lys Asn  Ser Ala Asn Tyr Ser  Glu Lys Leu 
    1205                 1210                 1215             


Leu Glu  Ile Ile Lys Pro Gln  Val Met Phe Asn Lys  Lys Asn Gly 
    1220                 1225                 1230             


Lys Pro  Lys Lys Gly Val Asn  Asp Val Arg Ile Leu  Met Asp Asn 
    1235                 1240                 1245             


Ile Pro  Tyr Arg Gln Pro Val  Leu Glu Gly Gly Ala  Leu Tyr Met 
    1250                 1255                 1260             


Leu Gly  Ser Ser Thr Tyr Lys  Tyr Ser Leu Lys Gln  Ile Thr Leu 
    1265                 1270                 1275             


Ser Gln  Glu Ser Met Lys Tyr  Ile Leu Asp Tyr Ile  Asp Asp Pro 
    1280                 1285                 1290             


Ser Phe  Asn Lys His Ile Met  Val Asn Ile Asp His  Gln Asp Glu 
    1295                 1300                 1305             


Lys Lys  Cys Leu Leu Ser Val  Tyr Asp Glu Ile Leu  Asp Lys Met 
    1310                 1315                 1320             


Asp Lys  Tyr Leu Pro Leu Phe  Asp Ile Asn Gly Phe  Arg Lys Lys 
    1325                 1330                 1335             


Leu His  Asp Gly Arg Glu Lys  Phe Val Lys Leu Pro  Val Glu Lys 
    1340                 1345                 1350             


Gln Ala  Glu Val Val Lys Asp  Ile Leu Arg Gly Leu  His Ala Asn 
    1355                 1360                 1365             


Ser Glu  Met Thr Lys Pro Ser  Asp Leu Gly Leu Thr  Thr Ala Leu 
    1370                 1375                 1380             


Gly Phe  Met Gln Ala Pro Asp  Gly Ile Lys Ile Ser  Asp Asp Ala 
    1385                 1390                 1395             


Val Phe  Ile Tyr Gln Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Val 
    1400                 1405                 1410             


Lys Val  Ser Asp Leu Leu Lys  
    1415                 1420 


<210>  1215
<211>  1421
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1215

Met Thr Lys Phe Asn Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Val 
1               5                   10                  15      


Ser Ser Val Gly Tyr Ala Val Val Thr Glu Asp Tyr Arg Val Pro Ala 
            20                  25                  30          


Phe Lys Phe Lys Val Leu Gly Asn Thr Glu Lys Glu Lys Ile Lys Lys 
        35                  40                  45              


Asn Leu Ile Gly Ser Thr Thr Phe Val Ser Ala Gln Pro Ala Lys Gly 
    50                  55                  60                  


Thr Arg Val Phe Arg Val Asn Arg Arg Arg Ile Asp Arg Arg Asn His 
65                  70                  75                  80  


Arg Ile Thr Tyr Leu Arg Asp Ile Phe Gln Lys Glu Ile Glu Lys Val 
                85                  90                  95      


Asp Lys Asn Phe Tyr Arg Arg Leu Asp Glu Ser Phe Arg Val Leu Gly 
            100                 105                 110         


Asp Lys Ser Glu Asp Leu Gln Ile Lys Gln Pro Phe Phe Gly Asp Lys 
        115                 120                 125             


Glu Leu Glu Thr Ala Tyr His Lys Lys Tyr Pro Thr Ile Tyr His Leu 
    130                 135                 140                 


Arg Lys His Leu Ala Asp Ala Asp Lys Asn Ser Pro Val Ala Asp Ile 
145                 150                 155                 160 


Arg Glu Val Tyr Met Ala Ile Ser His Ile Leu Lys Tyr Arg Gly His 
                165                 170                 175     


Phe Leu Thr Leu Asp Lys Ile Asn Pro Asn Asn Ile Asn Met Gln Asn 
            180                 185                 190         


Ser Trp Ile Asp Phe Ile Glu Ser Cys Gln Glu Val Phe Asp Leu Glu 
        195                 200                 205             


Ile Ser Asp Glu Ser Lys Asn Ile Ala Asp Ile Phe Lys Ser Ser Glu 
    210                 215                 220                 


Asn Arg Gln Glu Lys Val Lys Lys Ile Leu Pro Tyr Phe Gln Gln Glu 
225                 230                 235                 240 


Leu Leu Lys Lys Asp Lys Ser Ile Phe Lys Gln Leu Leu Gln Leu Leu 
                245                 250                 255     


Phe Gly Leu Lys Thr Lys Phe Lys Asp Cys Phe Glu Leu Glu Glu Glu 
            260                 265                 270         


Pro Asp Leu Asn Phe Ser Lys Glu Asn Tyr Asp Glu Asn Leu Glu Asn 
        275                 280                 285             


Phe Leu Gly Ser Leu Glu Glu Asp Phe Ser Asp Val Phe Ala Lys Leu 
    290                 295                 300                 


Lys Val Leu Arg Asp Thr Ile Leu Leu Ser Gly Met Leu Thr Tyr Thr 
305                 310                 315                 320 


Gly Ala Thr His Ala Arg Phe Ser Ala Thr Met Val Glu Arg Tyr Glu 
                325                 330                 335     


Glu His Arg Lys Asp Leu Gln Arg Phe Lys Phe Phe Ile Lys Gln Asn 
            340                 345                 350         


Leu Ser Glu Gln Asp Tyr Leu Asp Ile Phe Gly Arg Lys Thr Gln Asn 
        355                 360                 365             


Gly Phe Asp Val Asp Lys Glu Thr Lys Gly Tyr Val Gly Tyr Ile Thr 
    370                 375                 380                 


Asn Lys Met Val Leu Thr Asn Pro Gln Lys Gln Lys Thr Ile Gln Gln 
385                 390                 395                 400 


Asn Phe Tyr Asp Tyr Ile Ser Gly Lys Ile Thr Gly Ile Glu Gly Ala 
                405                 410                 415     


Glu Tyr Phe Leu Asn Lys Ile Ser Asp Gly Thr Phe Leu Arg Lys Leu 
            420                 425                 430         


Arg Thr Ser Asp Asn Gly Ala Ile Pro Asn Gln Ile His Ala Tyr Glu 
        435                 440                 445             


Leu Glu Lys Ile Ile Glu Arg Gln Gly Lys Asp Tyr Pro Phe Leu Leu 
    450                 455                 460                 


Glu Asn Lys Asp Lys Leu Leu Ser Ile Leu Thr Phe Lys Ile Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Ala Lys Gly Ser Asn Ser Arg Phe Ala Trp Ile 
                485                 490                 495     


Lys Arg Ala Thr Ser Ser Asp Ile Leu Asp Asp Asn Asp Glu Asp Thr 
            500                 505                 510         


Arg Asn Gly Lys Ile Arg Pro Trp Asn Tyr Gln Lys Leu Ile Asn Met 
        515                 520                 525             


Asp Glu Thr Arg Asp Ala Phe Ile Thr Asn Leu Ile Gly Asn Asp Ile 
    530                 535                 540                 


Ile Leu Leu Asn Glu Lys Val Leu Pro Lys Arg Ser Leu Ile Tyr Glu 
545                 550                 555                 560 


Glu Val Met Leu Gln Asn Glu Leu Thr Arg Val Lys Tyr Lys Asp Lys 
                565                 570                 575     


Tyr Gly Lys Ala His Phe Phe Asp Ser Glu Leu Arg Gln Asn Ile Ile 
            580                 585                 590         


Asn Gly Leu Phe Lys Asn Asn Ser Lys Arg Val Asn Ala Lys Ser Leu 
        595                 600                 605             


Ile Lys Tyr Leu Ser Asp Asn His Lys Asp Leu Asn Ala Ile Glu Ile 
    610                 615                 620                 


Val Ser Gly Val Glu Lys Gly Lys Ser Phe Asn Ser Thr Leu Lys Thr 
625                 630                 635                 640 


Tyr Asn Asp Leu Lys Thr Ile Phe Ser Glu Glu Leu Leu Asp Ser Glu 
                645                 650                 655     


Ile Tyr Gln Lys Glu Leu Glu Glu Ile Ile Lys Val Ile Thr Val Phe 
            660                 665                 670         


Asp Asp Lys Lys Ser Ile Lys Asn Tyr Leu Thr Lys Phe Phe Gly His 
        675                 680                 685             


Leu Glu Ile Leu Asp Glu Glu Lys Ile Asn Gln Leu Ser Lys Leu Arg 
    690                 695                 700                 


Tyr Ser Gly Trp Gly Arg Tyr Ser Ala Lys Leu Leu Leu Asp Ile Arg 
705                 710                 715                 720 


Asp Glu Asp Thr Gly Phe Asn Leu Leu Gln Phe Leu Arg Asn Asp Glu 
                725                 730                 735     


Glu Asn Arg Asn Leu Thr Lys Leu Ile Ser Asp Asn Thr Leu Ser Phe 
            740                 745                 750         


Glu Pro Lys Ile Lys Asp Ile Gln Ser Lys Ser Thr Ile Glu Asp Asp 
        755                 760                 765             


Ile Phe Asp Glu Ile Lys Lys Leu Ala Gly Ser Pro Ala Ile Lys Arg 
    770                 775                 780                 


Gly Ile Leu Asn Ser Ile Lys Ile Val Asp Glu Leu Val Gln Ile Ile 
785                 790                 795                 800 


Gly Tyr Pro Pro His Asn Ile Val Ile Glu Met Ala Arg Glu Asn Met 
                805                 810                 815     


Thr Thr Glu Glu Gly Gln Lys Lys Ala Lys Thr Arg Lys Thr Lys Leu 
            820                 825                 830         


Glu Ser Ala Leu Lys Asn Ile Glu Asn Ser Leu Leu Glu Asn Gly Lys 
        835                 840                 845             


Val Pro His Ser Asp Glu Gln Leu Gln Ser Glu Lys Leu Tyr Leu Tyr 
    850                 855                 860                 


Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Leu Asp Lys Thr Gly Ser 
865                 870                 875                 880 


Pro Ala Pro Leu Tyr Leu Asp Gln Leu Asp Gln Tyr Glu Val Asp His 
                885                 890                 895     


Ile Ile Pro Tyr Ser Phe Leu Pro Ile Asp Ser Ile Asp Asn Lys Val 
            900                 905                 910         


Leu Thr His Arg Glu Asn Asn Gln Gln Lys Leu Asn Asn Ile Pro Asp 
        915                 920                 925             


Lys Glu Thr Val Ala Asn Met Lys Pro Phe Trp Glu Lys Leu Tyr Asn 
    930                 935                 940                 


Ala Lys Leu Ile Ser Gln Thr Lys Tyr Gln Arg Leu Thr Thr Ser Glu 
945                 950                 955                 960 


Arg Thr Pro Asp Gly Val Leu Thr Glu Ser Met Lys Ala Gly Phe Ile 
                965                 970                 975     


Glu Arg Gln Leu Val Glu Thr Arg Gln Ile Ile Lys His Val Ala Arg 
            980                 985                 990         


Ile Leu Asp Asn Arg Phe Ser Asp  Thr Lys Ile Ile Thr  Leu Lys Ser 
        995                 1000                 1005             


Gln Leu  Ile Thr Asn Phe Arg  Asn Thr Phe His Ile  Ala Lys Ile 
    1010                 1015                 1020             


Arg Glu  Leu Asn Asp Tyr His  His Ala His Asp Ala  Tyr Leu Ala 
    1025                 1030                 1035             


Val Val  Val Gly Gln Thr Leu  Leu Lys Val Tyr Pro  Lys Leu Ala 
    1040                 1045                 1050             


Pro Glu  Leu Ile Tyr Gly His  His Ala His Phe Asn  Arg His Glu 
    1055                 1060                 1065             


Glu Asn  Lys Ala Thr Leu Arg  Lys His Leu Tyr Ser  Asn Ile Met 
    1070                 1075                 1080             


Arg Phe  Phe Asn Asn Pro Asp  Ser Lys Val Ser Lys  Asp Ile Trp 
    1085                 1090                 1095             


Asp Cys  Asn Arg Asp Leu Pro  Ile Ile Lys Asp Val  Ile Tyr Asn 
    1100                 1105                 1110             


Ser Gln  Ile Asn Phe Val Lys  Arg Thr Met Ile Lys  Lys Gly Ala 
    1115                 1120                 1125             


Phe Tyr  Asn Gln Asn Pro Val  Gly Lys Phe Asn Lys  Gln Leu Ala 
    1130                 1135                 1140             


Ala Asn  Asn Arg Tyr Pro Leu  Lys Thr Lys Ala Leu  Cys Leu Asp 
    1145                 1150                 1155             


Thr Ser  Ile Tyr Gly Gly Tyr  Gly Pro Met Asn Ser  Ala Leu Ser 
    1160                 1165                 1170             


Ile Ile  Ile Ile Ala Glu Arg  Phe Asn Glu Lys Lys  Gly Lys Ile 
    1175                 1180                 1185             


Glu Thr  Val Lys Glu Phe His  Asp Ile Phe Ile Ile  Asp Tyr Glu 
    1190                 1195                 1200             


Lys Phe  Asn Asn Asn Pro Phe  Gln Phe Leu Asn Asp  Thr Ser Glu 
    1205                 1210                 1215             


Asn Gly  Phe Leu Lys Lys Asn  Asn Ile Asn Arg Val  Leu Gly Phe 
    1220                 1225                 1230             


Tyr Arg  Ile Pro Lys Tyr Ser  Leu Met Gln Lys Ile  Asp Gly Thr 
    1235                 1240                 1245             


Arg Met  Leu Phe Glu Ser Lys  Ser Asn Leu His Lys  Ala Thr Gln 
    1250                 1255                 1260             


Phe Lys  Leu Thr Lys Thr Gln  Asn Glu Leu Phe Phe  His Met Lys 
    1265                 1270                 1275             


Arg Leu  Leu Thr Lys Ser Asn  Leu Met Asp Leu Lys  Ser Lys Ser 
    1280                 1285                 1290             


Ala Ile  Lys Glu Ser Gln Asn  Phe Ile Leu Lys His  Lys Glu Glu 
    1295                 1300                 1305             


Phe Asp  Asn Ile Ser Asn Gln  Leu Ser Ala Phe Ser  Gln Lys Met 
    1310                 1315                 1320             


Leu Gly  Asn Thr Thr Ser Leu  Lys Asn Leu Ile Lys  Gly Tyr Asn 
    1325                 1330                 1335             


Glu Arg  Lys Ile Lys Glu Ile  Asp Ile Arg Asp Glu  Thr Ile Lys 
    1340                 1345                 1350             


Tyr Phe  Tyr Asp Asn Phe Ile  Lys Met Phe Ser Phe  Val Lys Ser 
    1355                 1360                 1365             


Gly Ala  Pro Lys Asp Ile Asn  Asp Phe Phe Asp Asn  Lys Cys Thr 
    1370                 1375                 1380             


Val Ala  Arg Met Arg Pro Lys  Pro Asp Lys Lys Leu  Leu Asn Ala 
    1385                 1390                 1395             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1400                 1405                 1410             


Asp Leu  Ser Lys Leu Gly Glu  Asp 
    1415                 1420     


<210>  1216
<211>  1421
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1216

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Pro Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Glu Asn Glu Lys Ser Ser Ile Ile 
            20                  25                  30          


Lys Ile Gly Ser Arg Val Ile His Tyr Gly Asp Asn Leu Val Lys Val 
        35                  40                  45              


Asp Lys Ser Gly Lys Ile Ser Ala Ser Met Thr Pro Glu Glu Asp Phe 
    50                  55                  60                  


Ser Ser Gly Lys Gly Leu Ser Pro Asn Ala Gly Arg Thr Lys Gln Arg 
65                  70                  75                  80  


Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Gln Arg Arg Asn Asn Leu 
                85                  90                  95      


Ile Glu Ile Leu Lys Ala His Ser Leu Ile Asp Asn Asn Phe Ile Phe 
            100                 105                 110         


Ser Glu Glu Gly Asn Glu Thr Thr Phe Lys Thr Tyr Lys Asn Arg Ala 
        115                 120                 125             


Leu Ala Ala Thr Asn Glu Ile Ser Leu Glu Asp Phe Ile Lys Val Leu 
    130                 135                 140                 


Phe Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala Lys 
145                 150                 155                 160 


Gly Asp Glu Asp Gly Gln Leu Ile Asp Gly Met Ala Ile Ala Ile Gln 
                165                 170                 175     


Leu Tyr Glu His Asn Gln Thr Pro Gly Gln Phe Val Tyr Asn Leu Leu 
            180                 185                 190         


Lys Asn Gly Lys Lys Asn Ile Pro Asp Phe Tyr Arg Ser Asp Leu Gln 
        195                 200                 205             


Thr Glu Phe Asp Arg Ile Trp Asn Phe Gln Lys Ala Phe His Gln Glu 
    210                 215                 220                 


Ser Leu Ser Asp Gly Ile Cys Glu Lys Leu Val Gly Gln Lys Lys Asn 
225                 230                 235                 240 


Val Thr Ser Ala Ile Leu Phe Lys Glu Phe Lys Ile Glu Ser Leu Glu 
                245                 250                 255     


Ile Lys Gly Lys Trp Asp Glu Lys Arg Phe Phe Arg Tyr Glu Leu Arg 
            260                 265                 270         


Asn Lys Ala Val Gln Gln Glu Leu Lys Leu Asn Glu Ile Ala Glu Cys 
        275                 280                 285             


Leu Val Glu Ile Asn Gly Glu Ile Ser Asn Ser Ser Gly Tyr Leu Gly 
    290                 295                 300                 


Ala Ile Ser Asp Arg Ser Lys Glu Leu Val Phe Gln Lys Ile Thr Val 
305                 310                 315                 320 


Gly Gln Tyr Leu Tyr Lys Gln Ile Ala Ala Asn Pro Asn Thr Ser Leu 
                325                 330                 335     


Lys Lys Gln Ile Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Asn Thr 
            340                 345                 350         


Leu Trp Glu Thr Gln Ala Lys Tyr His Lys Glu Leu Thr Asp Glu Leu 
        355                 360                 365             


Lys Thr Gln Ile Arg Asp Val Ile Ile Phe Tyr Gln Arg Arg Leu Lys 
    370                 375                 380                 


Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Lys Gln Ile 
385                 390                 395                 400 


Glu Val Phe Val Asp Gly Lys Lys Ser Ile Lys Thr Arg Gly Met Arg 
                405                 410                 415     


Val Ile Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln 
            420                 425                 430         


Ile Leu Asn Asn Ile Glu Ile Thr Asp Leu Lys Thr Lys Glu Ser Arg 
        435                 440                 445             


Glu Leu Thr Asp Asp Glu Arg Gln Leu Leu Phe Glu Glu Leu Asn Ile 
    450                 455                 460                 


Arg Pro Lys Ile Ser Lys Lys Glu Ala Leu Lys Ile Leu Thr Glu Lys 
465                 470                 475                 480 


Pro Ala Gln Tyr Asp Leu Asn Tyr Lys Asp Ile Glu Gly Asn Lys Thr 
                485                 490                 495     


Asn Ala Lys Phe Tyr Glu Ala Tyr Asn Lys Ile Ile His Leu Ser Gly 
            500                 505                 510         


His Asp Glu Ile Asp Phe Thr Asp Lys Thr Ser Asp Ile Asn Glu Tyr 
        515                 520                 525             


Leu Lys Gln Ile Phe Ser Leu Leu Gly Ile Lys Pro Glu Ile Leu Asp 
    530                 535                 540                 


Phe Asp Ser Ser Leu Lys Asp Glu Asp Phe Glu Lys Gln Leu His Phe 
545                 550                 555                 560 


Gln Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Lys Thr 
                565                 570                 575     


Gly Asp Glu Lys Leu Leu Gln Lys Leu Asn Thr Lys Phe Gly Phe Pro 
            580                 585                 590         


Val Glu Tyr Ala Lys Val Ile Ser Asn Ile Ala Leu Pro Asp Asp Tyr 
        595                 600                 605             


Gly Ser Leu Ser Ala Lys Ala Ile Arg Lys Ile Leu Pro His Leu Lys 
    610                 615                 620                 


Asp Gly Phe Ser Tyr Gly Gly Arg Lys Ser Arg Pro Asp Glu Pro Ser 
625                 630                 635                 640 


Ala Cys Glu Tyr Ala Gly Tyr Arg His Ser Lys Ala Ser Leu Thr Lys 
                645                 650                 655     


Asp Glu Lys Glu Asn Lys Thr Tyr Lys Asp Lys Leu Glu Ile Leu Pro 
            660                 665                 670         


Lys Asn Ser Leu Arg Asn Pro Ile Val Glu Lys Ile Leu Asn Gln Thr 
        675                 680                 685             


Ile Asn Val Val Asn Ala Val Ile Gly Asp Tyr Gly Lys Pro Asp Glu 
    690                 695                 700                 


Ile Arg Leu Glu Leu Ala Arg Glu Leu Lys Lys Ser Ala Glu Glu Arg 
705                 710                 715                 720 


Gln Asp Ala Thr Asp Ser Ile Ser Lys Ala Thr Ala Lys His Glu Lys 
                725                 730                 735     


Tyr Arg Lys Val Leu Gln Thr Glu Phe Gly Leu Lys His Ile Ser Arg 
            740                 745                 750         


Asn Asp Leu Ile Arg Tyr Lys Leu Tyr Leu Glu Leu Lys Asn Asn Gly 
        755                 760                 765             


Phe Lys Thr Leu Tyr Ser Asp Thr Tyr Ile Ala Pro Tyr Glu Ile Phe 
    770                 775                 780                 


Ser Lys Lys Phe Asp Ile Glu His Ile Leu Pro Lys Ala Lys Leu Phe 
785                 790                 795                 800 


Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu Leu Lys Ser Val Asn Leu 
                805                 810                 815     


Lys Lys Gly Asp Met Thr Ala Tyr Asp Phe Val Lys Glu Glu Tyr Gly 
            820                 825                 830         


Thr Glu Gly Leu Ala Lys Tyr Glu Gln Arg Val Gln Asp Leu Phe Leu 
        835                 840                 845             


Asn Lys Lys Arg Asn Glu Asp Leu Asp Gln Glu Asn Glu Ser Arg Ile 
    850                 855                 860                 


Thr Asp Glu Lys Ile Ser Lys Thr Lys Arg Asp Lys Leu Leu Met Pro 
865                 870                 875                 880 


Glu Asn Lys Ile Pro Thr Gly Phe Ile Asp Arg Glu Leu Arg Asp Ser 
                885                 890                 895     


Gln Tyr Ile Ala Lys Lys Ala Lys Thr Met Met Glu Asp Ile Cys Lys 
            900                 905                 910         


Asp Val Val Thr Thr Thr Gly Ser Val Thr Asp Arg Leu Arg Glu Asp 
        915                 920                 925             


Trp Gln Leu Ile Asp Ile Met Gln Glu Leu Asn Trp Pro Lys Tyr Ala 
    930                 935                 940                 


Ala Leu Gly Leu Thr Glu Glu Phe Thr Asn Glu Glu Gly Asn Val Ile 
945                 950                 955                 960 


Arg Lys Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala 
                965                 970                 975     


Met Asp Ala Ile Thr Ile Ala Phe Thr Lys Arg Ser His Ile Gln Tyr 
            980                 985                 990         


Leu Asn Asn Leu Ser Ala Arg Ser  Asp Lys Ser Ser Ser  Ile Tyr Gly 
        995                 1000                 1005             


Ile Glu  Gln Lys Glu Leu Tyr  Arg Asp Lys Asn Asn  Lys Leu Arg 
    1010                 1015                 1020             


Phe Ala  Pro Pro Ile Pro Ile  Asn Glu Phe Arg Ala  Glu Ala Lys 
    1025                 1030                 1035             


Met Gln  Leu Glu Asn Thr Leu  Ile Ser Ile Lys Ala  Gln Asn Lys 
    1040                 1045                 1050             


Val Val  Thr Lys Asn Lys Asn  Lys Ile Ser Gly Gln  Lys Lys Leu 
    1055                 1060                 1065             


Gln Glu  Thr Leu Thr Pro Arg  Gly Gln Leu His Leu  Glu Thr Ile 
    1070                 1075                 1080             


Tyr Gly  Lys Ile Asn Gln Tyr  Gln Thr Ala Glu Ile  Lys Val Gly 
    1085                 1090                 1095             


Gly Thr  Leu Asp Ser Glu Leu  Ile Asn Lys Val Ala  Asn Lys Lys 
    1100                 1105                 1110             


Thr Arg  Glu Ala Leu Leu Asn  Arg Leu Ala Glu Phe  Gly Asn Asp 
    1115                 1120                 1125             


Pro Lys  Lys Ala Phe Thr Val  Lys Asn Thr Ile Asp  Lys Asn Pro 
    1130                 1135                 1140             


Ile Tyr  Leu Asp Lys His Gln  Ser Tyr Lys Ile Pro  Glu Lys Val 
    1145                 1150                 1155             


Lys Ile  Val Trp Gln Glu Pro  Val Tyr Thr Ile Arg  Lys Ser Ile 
    1160                 1165                 1170             


Thr Pro  Asp Leu Lys Leu Asp  Lys Ile Ile Asp Pro  Lys Ile Lys 
    1175                 1180                 1185             


Ala Leu  Leu Glu Ser Arg Leu  Lys Glu Cys Asn Gly  Asp Ala Lys 
    1190                 1195                 1200             


Leu Ala  Phe Ser Asn Leu Ala  Glu Asn Pro Ile Trp  Leu Asn Lys 
    1205                 1210                 1215             


Glu Lys  Gly Ile Gln Ile Lys  Arg Val Thr Ile Thr  Gly Ile Ser 
    1220                 1225                 1230             


Asn Ala  Ala Ser Leu His Asp  Lys Lys Asp Leu Asn  Gly Lys Pro 
    1235                 1240                 1245             


Ile Leu  Asp Glu Lys Gly Asn  Lys Ile Ser Val Asp  Phe Val Asn 
    1250                 1255                 1260             


Thr Gly  Asn Asn His His Val  Ala Ile Tyr Lys Asp  Glu Asn Gly 
    1265                 1270                 1275             


Asn Leu  His Asp Asn Val Val  Thr Phe Phe Glu Ala  Val Glu Arg 
    1280                 1285                 1290             


Val Asn  Gln Asn Leu Pro Val  Val Asp Lys Leu Phe  Lys Gln Lys 
    1295                 1300                 1305             


Glu Gly  Trp Gln Phe Leu Phe  Thr Met Lys Gln Asn  Glu Tyr Phe 
    1310                 1315                 1320             


Val Phe  Pro Asn Glu Ile Thr  Ala Phe Asn Pro Asn  Glu Phe Asp 
    1325                 1330                 1335             


Leu Leu  Asn Pro Asp Asn Tyr  Tyr Leu Ile Ser Pro  Asn Leu Phe 
    1340                 1345                 1350             


Arg Val  Gln Thr Met Ser Lys  Val Met Tyr Gly Asn  Asn Thr Leu 
    1355                 1360                 1365             


Arg Asp  Tyr Lys Phe Arg His  His Leu Glu Thr Thr  Leu Gln Asp 
    1370                 1375                 1380             


Ile Lys  Glu Leu Arg Asp Ile  Val Phe Lys Gln Phe  Lys Ser Leu 
    1385                 1390                 1395             


Ser Ile  Phe Asp Lys Ile Val  Lys Val Arg Ile Asn  His Leu Gly 
    1400                 1405                 1410             


Lys Ile  Val Lys Thr Gly Glu  Tyr 
    1415                 1420     


<210>  1217
<211>  1421
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1217

Met Lys Asp Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Lys Glu Ala Gln Asn Asp Asn Glu Gln Ser Glu Ile Val 
            20                  25                  30          


Asp Val Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Pro Ile Ser Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Lys Glu 
65                  70                  75                  80  


Leu Ile Glu Leu Leu Lys Glu His Gln Leu Ile Ser Thr Asp Thr Ile 
                85                  90                  95      


Leu Thr Glu Asn Gly Lys Gly Thr Thr Phe Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Lys Glu Lys Ile Ser Leu Glu Glu Leu Ala Arg Val 
        115                 120                 125             


Phe Leu Thr Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Glu Asp Glu Gly Gln Leu Ile Asp Gly Met Glu Ile Ala Lys 
145                 150                 155                 160 


Arg Leu Tyr Glu Glu Asn Leu Thr Thr Gly Gln Leu Ser Leu Gln Leu 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Ser Leu Pro Asp Tyr Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Phe Glu Leu Asp Lys Val Trp Asn Phe Gln Lys Gln Phe His Ser 
        195                 200                 205             


His Ile Leu Thr Asp Glu Phe Tyr Ala Gln Leu Lys Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ser Ala Leu Phe Leu Ala Lys Tyr Gln Ile Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Ser Arg Glu Asp Lys Lys Leu Gln Ala Tyr Gln Trp 
                245                 250                 255     


Arg Val Asp Ala Leu Ser Lys Glu Leu Thr Arg Glu Gln Leu Ala Phe 
            260                 265                 270         


Val Ile Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Glu Thr 
    290                 295                 300                 


Val Gly Glu Tyr Leu Trp Lys Gln Ile Ala Ala Asn Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Lys Thr Gln Ser Ala Phe Tyr Pro Glu Ile Leu Thr Glu 
            340                 345                 350         


Ser Leu Lys Lys Glu Leu Arg Asp Val Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Ser Leu Val Ser Phe Cys Glu Phe Glu Asn Lys 
    370                 375                 380                 


Glu Ile Glu Ile Lys Lys Glu Asp Gly Thr Ser Thr Lys Lys Val Ile 
385                 390                 395                 400 


Gly Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys 
                405                 410                 415     


Ile Trp Gln Asn Leu Asn Asn Val Leu Ile Arg Lys Lys Gly Ser Lys 
            420                 425                 430         


Thr Lys Lys Ala Ala Lys Asn Gln Gln Ala Val Leu Phe Glu Glu Glu 
        435                 440                 445             


Lys Thr Ile Ser Glu Leu Asp Leu Glu Ala Lys Gln Ala Leu Phe Asn 
    450                 455                 460                 


Glu Leu Asn Leu Lys Gly Asn Leu Lys Ala Asp Tyr Cys Leu Glu Ile 
465                 470                 475                 480 


Leu Gly Tyr Ser Pro Lys Glu Trp Glu Met Asn Tyr Ser Val Leu Glu 
                485                 490                 495     


Gly Asn Arg Thr Asn Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Thr 
            500                 505                 510         


Asp Leu Glu Gly Tyr Asp Ala Lys Asn His Leu Lys Val Lys Leu Asn 
        515                 520                 525             


Lys Asp Glu Val Glu Leu Asp Asp Leu Lys Val Pro Ala Ser Glu Ile 
    530                 535                 540                 


Lys Asp Met Val Gln Ala Ile Phe Lys Glu Ala Gly Ile Asn Thr Asp 
545                 550                 555                 560 


Ile Leu His Phe Asn Ala Glu Leu Asp Gly Lys Asp Phe Glu Asn Gln 
                565                 570                 575     


Ala Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp 
            580                 585                 590         


Ser Lys Ser Gly Asn Glu Leu Leu Tyr Lys Leu Leu Glu Thr Lys Phe 
        595                 600                 605             


Gly Phe Lys Lys Glu Tyr Ala Gln Ile Leu Ala Asn Val Ser Leu Ser 
    610                 615                 620                 


Asp Asp Tyr Gly Ser Leu Ser Thr Lys Ala Met Arg Lys Ile His Pro 
625                 630                 635                 640 


Tyr Ile Lys Glu Asn Lys Phe Ser Thr Ala Cys Glu Leu Ala Gly Tyr 
                645                 650                 655     


Arg His Ser Lys His Ser Leu Thr Lys Glu Glu Asn Asp Asn Arg Val 
            660                 665                 670         


Leu Arg Asp Ser Leu Glu Val Leu Lys Lys Asn Ser Leu Arg Gln Pro 
        675                 680                 685             


Val Val Glu Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Ala Leu 
    690                 695                 700                 


Leu Lys Lys Tyr Arg Thr Glu Asn Pro Asn Phe Lys Phe Asp Glu Ile 
705                 710                 715                 720 


Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Glu Glu Arg Ala 
                725                 730                 735     


Asn Met Thr Ala Asn Ile Asn Lys Ala Lys Thr Glu His Glu Lys Ile 
            740                 745                 750         


Ile Lys Ile Leu Lys Asn Glu Phe Gly Leu Pro Asn Pro Thr Arg Asn 
        755                 760                 765             


Asp Ile Ile Arg Tyr Arg Leu Tyr Glu Glu Leu Lys Ala Asn Gly Tyr 
    770                 775                 780                 


Lys Asp Leu Tyr Thr Asn Ile Lys Ile Pro Gln Glu Lys Leu Phe Thr 
785                 790                 795                 800 


Lys Glu Ile Asp Ile Glu His Ile Ile Pro Lys Ser Arg Val Phe Asp 
                805                 810                 815     


Asp Ser Phe Ser Asn Lys Thr Leu Ser Phe Arg Lys Thr Asn Leu Asp 
            820                 825                 830         


Lys Gly Glu Arg Thr Ala Phe Asp Tyr Met Glu Ser Lys Phe Gly Ala 
        835                 840                 845             


Asp Lys Leu Glu Glu Tyr Val Ala Arg Val Glu Asp Leu Tyr Lys Asn 
    850                 855                 860                 


Asn Ile Ile Ser Lys Ala Lys Tyr Gln Lys Leu Leu Lys Lys Glu Ser 
865                 870                 875                 880 


Glu Ile Gly Asp Gly Phe Val Glu Arg Asp Leu Arg Glu Thr Gln Tyr 
                885                 890                 895     


Ile Ala Lys Lys Ala Lys Glu Ile Leu Leu Gln Ile Thr Pro Asn Val 
            900                 905                 910         


Leu Ser Thr Ser Gly Gly Ile Thr Asp Arg Leu Arg Glu Asp Trp Asp 
        915                 920                 925             


Leu Ile Asn Val Met Lys Glu Leu Asn Leu Pro Lys Tyr Arg Ala Leu 
    930                 935                 940                 


Gly Leu Thr Glu Met Glu Asp Arg Lys Tyr Gly Gln Gln Val Glu Val 
945                 950                 955                 960 


Ile Ile Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp 
                965                 970                 975     


Ala Leu Thr Val Ala Phe Thr Lys His Asn His Ile Gln Tyr Leu Asn 
            980                 985                 990         


Tyr Leu Asn Ala Arg Lys Asn Glu  Lys His Lys Glu His  Ser Asn Ile 
        995                 1000                 1005             


Met Gly  Ile Gln Gln Leu Glu  Thr Ile Lys Val Thr  Asp Lys Asn 
    1010                 1015                 1020             


Gly Asn  Glu Lys Arg Val Phe  Lys Ala Pro Met Pro  Asn Phe Arg 
    1025                 1030                 1035             


Gln Val  Thr Lys Ala Phe Leu  Glu Asn Ile Leu Ile  Ser His Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Val Thr  Lys Asn Lys Asn Lys  Val Ala Gly 
    1055                 1060                 1065             


Ser Asn  Lys Val Gln Glu Val  Leu Thr Pro Arg Gly  Gln Leu His 
    1070                 1075                 1080             


Lys Glu  Thr Val Tyr Gly Lys  Tyr Gln Tyr Tyr Val  Gln Lys Glu 
    1085                 1090                 1095             


Glu Lys  Ile Gly Gly Lys Phe  Asp Leu Glu Thr Ile  Lys Lys Val 
    1100                 1105                 1110             


Ala Asn  Pro Leu Tyr Gln Ser  Leu Leu Lys Lys Arg  Leu Glu Glu 
    1115                 1120                 1125             


Asn Gly  Gly Asp Pro Lys Lys  Ala Phe Thr Gly Lys  Asn Ser Leu 
    1130                 1135                 1140             


Ala Lys  Asn Pro Ile Tyr Leu  Asp Asp Ala Gln Thr  Glu Gln Leu 
    1145                 1150                 1155             


Pro Glu  Lys Val Lys Leu Val  Trp Leu Glu Asn Ser  Phe Ser Ile 
    1160                 1165                 1170             


Arg Lys  Asp Ile Thr Pro Glu  Asn Phe Lys Asp Glu  Lys Leu Ile 
    1175                 1180                 1185             


Asp Lys  Ile Leu Asp Glu Gly  Val Lys Arg Ile Leu  Lys Glu Arg 
    1190                 1195                 1200             


Leu Gln  Ser Phe Gly Asn Asp  Ala Lys Lys Ala Phe  Ser Asp Leu 
    1205                 1210                 1215             


Glu Lys  Asn Pro Ile Trp Leu  Asn Lys Glu Lys Gly  Ile Thr Ile 
    1220                 1225                 1230             


Lys Arg  Val Thr Ile Ser Gly  Val Ala Asn Ala Glu  Pro Leu His 
    1235                 1240                 1245             


Thr Lys  Lys Asp His Leu Gly  Lys Glu Ile Leu Asp  Lys Glu Gly 
    1250                 1255                 1260             


Lys Pro  Ile Pro Val Asp Phe  Ile Ser Thr Gly Ser  Asn His His 
    1265                 1270                 1275             


Val Ala  Val Tyr Arg Asp Glu  Lys Gly Asn Leu Gln  Glu Lys Val 
    1280                 1285                 1290             


Val Ser  Val Phe Glu Ala Val  Val Arg Ala Asn Gln  Gly Leu Pro 
    1295                 1300                 1305             


Ile Ile  Asp Lys Thr Tyr Asn  Gln His Leu Gly Trp  Glu Phe Leu 
    1310                 1315                 1320             


Phe Thr  Met Lys Gln Asn Glu  Met Phe Val Phe Pro  Asn Glu Ser 
    1325                 1330                 1335             


Thr Gly  Phe Asn Pro Asn Asp  Ile Asp Leu Leu Asp  Pro Lys Asn 
    1340                 1345                 1350             


Lys Arg  Ile Ile Ser Pro Asn  Leu Phe Arg Val Gln  Lys Leu Ala 
    1355                 1360                 1365             


Thr Gly  Asp Tyr Trp Phe Arg  His His Leu Glu Thr  Thr Val Glu 
    1370                 1375                 1380             


Thr Lys  Ser Ala Thr Lys Asp  Met Thr Tyr Lys Arg  Leu Ser Leu 
    1385                 1390                 1395             


Asn His  Ile Lys Asn Ile Ile  Lys Val Arg Ile Ser  His Leu Gly 
    1400                 1405                 1410             


Asp Ile  Val Ser Val Gly Glu  Tyr 
    1415                 1420     


<210>  1218
<211>  1424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1218

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Thr Asp Glu Glu Val Ser Ser Ile Ile 
            20                  25                  30          


Arg Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Thr Gln Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Glu Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Asp Ala 
65                  70                  75                  80  


Leu Val Glu Phe Leu Lys Glu Leu Gly Phe Ile Thr Asp Glu Thr Ile 
                85                  90                  95      


Leu Ser Glu Gln Gly Asn Arg Thr Thr Phe Glu Thr Cys Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Glu Glu Glu Ile Ser Leu Glu Glu Leu Ser Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Val 
    130                 135                 140                 


Lys Lys Glu Glu Asp Gly Val Leu Ile Asp Gly Met Glu Ile Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Glu Glu Asp Leu Thr Pro Gly Gln Leu Cys Ser Gln Leu 
                165                 170                 175     


Leu Glu Ser Gly Lys Lys Val Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Glu Glu Leu Asp Arg Ile Trp Ser Val Gln Lys Gln Phe His Pro 
        195                 200                 205             


Asp Val Phe Cys Asp Ala Ala Lys Glu Glu Ile Lys Gly Lys Asn Arg 
    210                 215                 220                 


Ser Gln Thr Trp Ala Ile Leu Ala Asn Tyr Phe Val Trp Lys Glu Glu 
225                 230                 235                 240 


Val Val Gly Trp Asn Asp Arg Glu Ala Lys Asn Glu Thr Ile Glu Lys 
                245                 250                 255     


Glu Cys Lys Leu Val Gly Leu Lys Arg Thr Thr Lys Gly Tyr Glu Leu 
            260                 265                 270         


Lys Lys Glu Asn Tyr Arg Trp Arg Ala Gln Ala Leu Thr Glu Gln Leu 
        275                 280                 285             


Gly Leu Glu Glu Ile Ala Val Val Leu Gln Glu Ile Asn Gly Gln Ile 
    290                 295                 300                 


Asn Ala Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Val 
305                 310                 315                 320 


Leu Tyr Phe Asn His Gln Thr Val Gly Gln Tyr Gln Met Ala Glu Leu 
                325                 330                 335     


Asp Lys Asn Pro His Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asp Arg Ile Trp Glu Lys Gln Ala Glu Phe 
        355                 360                 365             


His Lys Glu Leu Thr Ala Glu Leu Lys Lys Glu Ile Arg Asp Met Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Ser Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Arg Leu Glu Ile Val Val Glu Lys Asp Gly Lys Lys 
                405                 410                 415     


Gln Thr Lys Val Ile Gly Cys Lys Val Ile Pro Arg Ser His Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Val Trp Gln Thr Leu Asn Asp Ile Lys Val Leu 
        435                 440                 445             


Gly Arg Glu Lys Lys Arg Asn Ala Leu Ser Ala Ser Glu Ser Arg Ala 
    450                 455                 460                 


Leu Tyr Pro Glu Glu Lys Glu Ile Leu Ala Arg Glu Leu Ala Ile Lys 
465                 470                 475                 480 


Glu Asn Met Lys Lys Ala Asp Val Leu Lys Leu Leu Phe Glu Asn Pro 
                485                 490                 495     


Gln Glu Leu Asp Leu Asn Phe Lys Gln Ile Asp Gly Asn Arg Thr Gly 
            500                 505                 510         


Phe Ser Leu Phe Ser Ala Tyr Ser Lys Met Ile Glu Lys Tyr Gly Tyr 
        515                 520                 525             


Glu Pro Leu Asp Phe Lys Lys Pro Ala Asp Glu Ile Ile Gly Gln Leu 
    530                 535                 540                 


Arg Thr Ile Phe Thr Asp Leu Gly Trp Asn Thr Asn Leu Phe Thr Ile 
545                 550                 555                 560 


Asp Leu Thr Lys Glu Gly Lys Glu Leu Glu Met Gln Pro Tyr Phe Arg 
                565                 570                 575     


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Ser Thr Gly 
            580                 585                 590         


Asn Gly Lys Leu Ile Glu Lys Ile Met Gln Leu Cys Gly Val Glu Lys 
        595                 600                 605             


Glu Tyr Ala Val Glu Leu Ala Ser Leu Ser Phe Gln Asp Asp Tyr Gly 
    610                 615                 620                 


Ser Leu Ser Ala Lys Ala Ile Lys Arg Ile Leu Pro Tyr Leu Lys Glu 
625                 630                 635                 640 


Gly Asn Leu Tyr Asp Val Ala Cys Glu Tyr Ala Gly Tyr Arg His Ser 
                645                 650                 655     


Lys Ser Ser Leu Thr Lys Glu Glu Ile Glu Asn Lys Val Leu Lys Gly 
            660                 665                 670         


Lys Leu Glu Val Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
        675                 680                 685             


Lys Ile Leu Ser Gln Met Val Asn Val Ile Asn Thr Ile Ile Asp Thr 
    690                 695                 700                 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
705                 710                 715                 720 


Lys Ser Ala Lys Glu Arg Glu Glu Leu Thr Lys Ala Ile Ala Lys Ser 
                725                 730                 735     


Thr Arg Glu His Glu Glu Ile Arg Lys Leu Leu Gln Asp Glu Phe Gly 
            740                 745                 750         


Met Met Asn Val Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Glu 
        755                 760                 765             


Glu Leu Lys Asp Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Gln Tyr Ile 
    770                 775                 780                 


Pro Lys Glu Lys Ile Phe Ser Lys Glu Ile Asp Ile Glu His Ile Ile 
785                 790                 795                 800 


Pro Gln Ser Arg Leu Phe Asp Asp Ser Leu Ser Asn Lys Thr Leu Glu 
                805                 810                 815     


Tyr Lys Ala Ile Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
            820                 825                 830         


Val Lys Glu Lys Tyr Gly Glu Glu Gly Leu Gln Gln Tyr Ile Asn Arg 
        835                 840                 845             


Cys Glu Ser Leu Phe Asn Asp Lys Lys Ala Lys Leu Arg Lys Leu Lys 
    850                 855                 860                 


Met Glu Gln Lys Asp Ile Pro Asp Gly Phe Ile Asp Arg Asp Leu Arg 
865                 870                 875                 880 


Asn Thr Gln Tyr Ile Ala Lys Lys Ala Leu Ala Met Leu Asn Glu Ile 
                885                 890                 895     


Cys Arg Arg Val Val Ala Thr Thr Gly Ser Ile Thr Asp Gln Leu Arg 
            900                 905                 910         


Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn Leu Pro Lys 
        915                 920                 925             


Tyr Glu Ala Leu Gly Phe Val Glu Thr Tyr Glu Asp Lys Asp Gly Arg 
    930                 935                 940                 


Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His 
945                 950                 955                 960 


His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Asp Ala Phe Ile 
                965                 970                 975     


Gln Tyr Phe Asn Asn Lys Asn Ala Ala Gln Asp Ser Gly Asn Arg Asn 
            980                 985                 990         


His Thr Asn Ile Ile Gly Ile Lys  Thr Arg Tyr Phe Asp  Lys Gly Arg 
        995                 1000                 1005             


Ala Leu  Pro Pro Ile Pro Leu  Glu Gln Phe Arg Ile  Glu Ala Lys 
    1010                 1015                 1020             


Gln His  Leu Glu Lys Leu Leu  Val Ser Ile Lys Ala  Lys Asn Lys 
    1025                 1030                 1035             


Val Val  Thr Val Asn Val Asn  Arg Thr Lys Lys Arg  Lys Gly Glu 
    1040                 1045                 1050             


Asn Thr  Lys Ile Gln Gln Thr  Pro Arg Gly Gln Leu  His Leu Glu 
    1055                 1060                 1065             


Thr Val  Tyr Gly Ser His Lys  Gln Tyr Val Thr Lys  Ile Glu Lys 
    1070                 1075                 1080             


Val Asn  Ala Ser Phe Asp Ala  Ala Lys Ile Ala Thr  Val Ser Lys 
    1085                 1090                 1095             


Arg Ala  Tyr Arg Asn Ala Leu  Leu Lys Arg Leu Glu  Thr Phe Gly 
    1100                 1105                 1110             


Asn Asp  Pro Lys Lys Ala Phe  Thr Gly Lys Asn Ala  Leu Glu Lys 
    1115                 1120                 1125             


Asn Pro  Leu Tyr Ala Asp Lys  Tyr Gln Thr Ile Arg  Val Pro Glu 
    1130                 1135                 1140             


Lys Val  Gln Thr Val Glu Phe  Glu Thr Ile Tyr Thr  Ile Arg Lys 
    1145                 1150                 1155             


Pro Val  Asp Pro Ala Leu Asn  Val Asp Lys Val Val  Asp Val Lys 
    1160                 1165                 1170             


Val Arg  Ala Ile Leu Glu Arg  Arg Leu Lys Glu Tyr  Gly Gly Asp 
    1175                 1180                 1185             


Pro Lys  Lys Ala Phe Val Asn  Phe Trp Glu Asn Pro  Ile Trp Leu 
    1190                 1195                 1200             


Asn Lys  Glu Lys Gly Ile Ser  Ile Lys Arg Val Ser  Ile Arg Gly 
    1205                 1210                 1215             


Ile Asn  Asn Ala Gln Ser Ile  His Val Lys Lys Asp  Lys Asn Gly 
    1220                 1225                 1230             


Asn Pro  Ile Trp Asp Glu Asn  Gly Lys Gln Ile Pro  Val Asp Phe 
    1235                 1240                 1245             


Val Asn  Thr Gly Asn Asn His  His Val Ala Ile Tyr  Arg Lys Pro 
    1250                 1255                 1260             


Val Leu  Asp Lys Lys Gly Gln  Val Thr Phe Asp Glu  Asp Gly Asn 
    1265                 1270                 1275             


Leu Met  Tyr Glu Leu Asp Glu  Val Val Val Pro Phe  Phe Glu Ala 
    1280                 1285                 1290             


Val Thr  Arg Ala Asn Leu Gly  Leu Pro Ile Ile Asp  Lys Asp Tyr 
    1295                 1300                 1305             


Arg Lys  Ser Glu Gly Trp Gln  Phe Leu Phe Ser Met  Lys Gln Asn 
    1310                 1315                 1320             


Glu Tyr  Phe Val Phe Pro Asn  Glu Lys Thr Gly Phe  Asn Pro Lys 
    1325                 1330                 1335             


Glu Val  Asp Leu Leu Asn Pro  Asp Asn Tyr Ala Met  Ile Ser Pro 
    1340                 1345                 1350             


Asn Leu  Phe Arg Val Gln Thr  Met Ser Lys Val Met  Tyr Gly Asn 
    1355                 1360                 1365             


Asn Val  Val Arg Asp Tyr Lys  Phe Arg His His Leu  Glu Thr Thr 
    1370                 1375                 1380             


Val Lys  Asp Met Lys Glu Leu  Lys Asp Ile Ala Tyr  Lys Gln Tyr 
    1385                 1390                 1395             


Lys Thr  Leu Ser Phe Gly Asn  Ser Val Val Lys Ile  Arg Ile Asn 
    1400                 1405                 1410             


His Ile  Gly Gln Ile Val Ser  Val Gly Glu Tyr 
    1415                 1420                 


<210>  1219
<211>  1424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1219

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Thr Asp Glu Glu Val Ser Ser Ile Ile 
            20                  25                  30          


Arg Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Thr Gln Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Glu Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Asp Ala 
65                  70                  75                  80  


Leu Val Glu Phe Leu Lys Glu Leu Gly Phe Ile Thr Asp Glu Thr Ile 
                85                  90                  95      


Leu Ser Glu Gln Gly Asn Arg Thr Thr Phe Glu Thr Cys Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Glu Glu Glu Ile Ser Leu Glu Glu Leu Ser Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Val 
    130                 135                 140                 


Lys Lys Glu Glu Asp Gly Val Leu Ile Asp Gly Met Glu Ile Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Glu Glu Asp Leu Thr Pro Gly Gln Leu Cys Ser Gln Leu 
                165                 170                 175     


Leu Glu Ser Gly Lys Lys Val Leu Pro Asp Phe Tyr His Ser Asp Leu 
            180                 185                 190         


Gln Glu Glu Leu Asp Arg Ile Trp Ser Val Gln Lys Gln Phe His Pro 
        195                 200                 205             


Asp Val Phe Cys Asp Ala Ala Lys Glu Glu Ile Lys Gly Lys Asn Arg 
    210                 215                 220                 


Ser Gln Thr Trp Ala Ile Leu Ala Asn Tyr Phe Val Trp Lys Glu Glu 
225                 230                 235                 240 


Val Val Gly Trp Asn Asp Arg Glu Ala Lys Asn Glu Thr Ile Glu Lys 
                245                 250                 255     


Glu Cys Lys Leu Val Gly Leu Lys Arg Thr Thr Lys Gly Tyr Glu Leu 
            260                 265                 270         


Lys Lys Glu Asn Tyr Arg Trp Arg Ala Gln Ala Leu Thr Glu Gln Leu 
        275                 280                 285             


Gly Leu Glu Glu Ile Ala Val Val Leu Gln Glu Ile Asn Gly Gln Ile 
    290                 295                 300                 


Asn Ala Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Val 
305                 310                 315                 320 


Leu Tyr Phe Asn His Gln Thr Val Gly Gln Tyr Gln Met Ala Glu Leu 
                325                 330                 335     


Asp Lys Asn Pro His Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asp Arg Ile Trp Glu Lys Gln Ala Glu Phe 
        355                 360                 365             


His Lys Glu Leu Thr Ala Glu Leu Lys Lys Glu Ile Arg Asp Met Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Ser Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Arg Leu Glu Ile Val Val Glu Lys Asp Gly Lys Lys 
                405                 410                 415     


Gln Thr Lys Val Ile Gly Cys Lys Val Ile Pro Arg Ser His Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Val Trp Gln Thr Leu Asn Asp Ile Lys Val Leu 
        435                 440                 445             


Gly Arg Glu Lys Lys Arg Asn Ala Leu Ser Ala Ser Glu Ser Arg Ala 
    450                 455                 460                 


Leu Tyr Pro Glu Glu Lys Glu Ile Leu Ala Arg Glu Leu Ala Ile Lys 
465                 470                 475                 480 


Glu Asn Met Lys Lys Ala Asp Val Leu Lys Leu Leu Phe Glu Asn Pro 
                485                 490                 495     


Gln Glu Leu Asp Leu Asn Phe Lys Gln Ile Asp Gly Asn Arg Thr Gly 
            500                 505                 510         


Phe Ser Leu Phe Ser Ala Tyr Ser Lys Met Ile Glu Lys Tyr Gly Tyr 
        515                 520                 525             


Glu Pro Leu Asp Phe Lys Lys Pro Ala Asp Glu Ile Ile Gly Gln Leu 
    530                 535                 540                 


Arg Thr Ile Phe Thr Asp Leu Gly Trp Asn Thr Asn Leu Phe Thr Ile 
545                 550                 555                 560 


Asp Leu Thr Lys Glu Gly Lys Glu Leu Glu Met Gln Pro Tyr Phe Arg 
                565                 570                 575     


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Ser Thr Gly 
            580                 585                 590         


Asn Gly Lys Leu Ile Glu Lys Ile Met Gln Leu Cys Gly Val Glu Lys 
        595                 600                 605             


Glu Tyr Ala Val Glu Leu Ala Ser Leu Ser Phe Gln Asp Asp Tyr Gly 
    610                 615                 620                 


Ser Leu Ser Ala Lys Ala Ile Lys Arg Ile Leu Pro Tyr Leu Lys Glu 
625                 630                 635                 640 


Gly Asn Leu Tyr Asp Val Ala Cys Glu Tyr Ala Gly Tyr Arg His Ser 
                645                 650                 655     


Lys Ser Ser Leu Thr Lys Glu Glu Ile Glu Asn Lys Val Leu Lys Gly 
            660                 665                 670         


Lys Leu Glu Val Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
        675                 680                 685             


Lys Ile Leu Ser Gln Met Val Asn Val Ile Asn Thr Ile Ile Asp Thr 
    690                 695                 700                 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
705                 710                 715                 720 


Lys Ser Ala Lys Glu Arg Glu Glu Leu Thr Lys Ala Ile Ala Lys Ser 
                725                 730                 735     


Thr Arg Glu His Glu Glu Ile Arg Lys Leu Leu Gln Asp Glu Phe Gly 
            740                 745                 750         


Met Met Asn Val Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Glu 
        755                 760                 765             


Glu Leu Lys Asp Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Gln Tyr Ile 
    770                 775                 780                 


Pro Lys Glu Lys Ile Phe Ser Lys Glu Ile Asp Ile Glu His Ile Ile 
785                 790                 795                 800 


Pro Gln Ser Arg Leu Phe Asp Asp Ser Leu Ser Asn Lys Thr Leu Glu 
                805                 810                 815     


Tyr Lys Ala Ile Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
            820                 825                 830         


Val Lys Glu Lys Tyr Gly Glu Glu Gly Leu Gln Gln Tyr Ile Asn Arg 
        835                 840                 845             


Cys Glu Ser Leu Phe Asn Asp Lys Lys Ala Lys Leu Arg Lys Leu Lys 
    850                 855                 860                 


Met Glu Gln Lys Asp Ile Pro Asp Gly Phe Ile Asp Arg Asp Leu Arg 
865                 870                 875                 880 


Asn Thr Gln Tyr Ile Ala Lys Lys Ala Leu Ala Met Leu Asn Glu Ile 
                885                 890                 895     


Cys Arg Arg Val Val Ala Thr Thr Gly Ser Ile Thr Asp Gln Leu Arg 
            900                 905                 910         


Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn Leu Pro Lys 
        915                 920                 925             


Tyr Glu Ala Leu Gly Phe Val Glu Thr Tyr Glu Asp Lys Asp Gly Arg 
    930                 935                 940                 


Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His 
945                 950                 955                 960 


His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Asp Ala Phe Ile 
                965                 970                 975     


Gln Tyr Phe Asn Asn Lys Asn Ala Ala Gln Asp Ser Gly Asn Arg Asn 
            980                 985                 990         


His Thr Asn Ile Ile Gly Ile Lys  Thr Arg Tyr Phe Asp  Lys Gly Arg 
        995                 1000                 1005             


Ala Leu  Pro Pro Ile Pro Leu  Glu Gln Phe Arg Ile  Glu Ala Lys 
    1010                 1015                 1020             


Gln His  Leu Glu Lys Leu Leu  Val Ser Ile Lys Ala  Lys Asn Lys 
    1025                 1030                 1035             


Val Val  Thr Val Asn Val Asn  Arg Thr Lys Lys Arg  Lys Gly Glu 
    1040                 1045                 1050             


Asn Thr  Lys Ile Gln Gln Thr  Pro Arg Gly Gln Leu  His Leu Glu 
    1055                 1060                 1065             


Thr Val  Tyr Gly Ser His Lys  Gln Tyr Val Thr Lys  Ile Glu Lys 
    1070                 1075                 1080             


Val Asn  Ala Ser Phe Asp Ala  Ala Lys Ile Ala Thr  Val Ser Lys 
    1085                 1090                 1095             


Arg Ala  Tyr Arg Asn Ala Leu  Leu Lys Arg Leu Glu  Thr Phe Gly 
    1100                 1105                 1110             


Asn Asp  Pro Lys Lys Ala Phe  Thr Gly Lys Asn Ala  Leu Glu Lys 
    1115                 1120                 1125             


Asn Pro  Leu Tyr Ala Asp Lys  Tyr Gln Thr Ile Arg  Val Pro Glu 
    1130                 1135                 1140             


Lys Val  Gln Thr Val Glu Phe  Glu Thr Ile Tyr Thr  Ile Arg Lys 
    1145                 1150                 1155             


Pro Val  Asp Pro Ala Leu Asn  Val Asp Lys Val Val  Asp Val Lys 
    1160                 1165                 1170             


Val Arg  Ala Ile Leu Glu Arg  Arg Leu Lys Glu Tyr  Gly Gly Asp 
    1175                 1180                 1185             


Pro Lys  Lys Ala Phe Val Asn  Phe Trp Glu Asn Pro  Ile Trp Leu 
    1190                 1195                 1200             


Asn Lys  Glu Lys Gly Ile Ser  Ile Lys Arg Val Ser  Ile Arg Gly 
    1205                 1210                 1215             


Ile Asn  Asn Ala Gln Ser Ile  His Val Lys Lys Asp  Lys Asn Gly 
    1220                 1225                 1230             


Asn Pro  Ile Trp Asp Glu Asn  Gly Lys Gln Ile Pro  Val Asp Phe 
    1235                 1240                 1245             


Val Asn  Thr Gly Asn Asn His  His Val Ala Ile Tyr  Arg Lys Pro 
    1250                 1255                 1260             


Val Leu  Asp Lys Lys Gly Gln  Val Thr Phe Asp Glu  Asp Gly Asn 
    1265                 1270                 1275             


Leu Met  Tyr Glu Leu Asp Glu  Val Val Val Pro Phe  Phe Glu Ala 
    1280                 1285                 1290             


Val Thr  Arg Ala Asn Leu Gly  Leu Pro Ile Ile Asp  Lys Asp Tyr 
    1295                 1300                 1305             


Arg Lys  Ser Glu Gly Trp Gln  Phe Leu Phe Ser Met  Lys Gln Asn 
    1310                 1315                 1320             


Glu Tyr  Phe Val Phe Pro Asn  Glu Lys Thr Gly Phe  Asn Pro Lys 
    1325                 1330                 1335             


Glu Val  Asp Leu Leu Asn Pro  Asp Asn Tyr Ala Met  Ile Ser Pro 
    1340                 1345                 1350             


Asn Leu  Phe Arg Val Gln Thr  Met Ser Lys Val Met  Tyr Gly Asn 
    1355                 1360                 1365             


Asn Val  Val Arg Asp Tyr Lys  Phe Arg His His Leu  Glu Thr Thr 
    1370                 1375                 1380             


Val Lys  Asp Met Lys Glu Leu  Lys Asp Ile Ala Tyr  Lys Gln Tyr 
    1385                 1390                 1395             


Lys Thr  Leu Ser Phe Gly Asn  Ser Val Val Lys Ile  Arg Ile Asn 
    1400                 1405                 1410             


His Ile  Gly Gln Ile Val Ser  Val Gly Glu Tyr 
    1415                 1420                 


<210>  1220
<211>  1425
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1220

Met Arg Arg Asn Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly 
1               5                   10                  15      


Phe Ala Lys Val Ile Glu Asp Glu Asp Tyr Gln Asn Ser Ala Val Glu 
            20                  25                  30          


Leu Ile Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Gln Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Arg Pro Val Ser Val Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Asp Arg Tyr Gln Leu Arg Arg His His 
65                  70                  75                  80  


Leu Ile Glu Val Leu Lys Ala Asn His Ile Ile Thr Val Asp Thr Lys 
                85                  90                  95      


Leu Ala Glu Asp Gly Lys Asn Thr Thr His Ala Thr Tyr Ala Val Arg 
            100                 105                 110         


Ala Leu Ala Ala Thr Ala Glu Ile Pro Leu Glu Ala Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Glu Asp Glu Gly Gln Leu Ile Asp Gly Met Ala Val Ala Lys 
145                 150                 155                 160 


Arg Leu Tyr Glu Glu Asn Leu Thr Pro Gly Gln Leu Ser Tyr Leu Leu 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Tyr Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Asn Ala Glu Leu Asp Lys Val Trp Ser Phe Gln Lys Gln Phe Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Glu Glu Phe Lys Lys His Leu Glu Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ser Ala Ala Phe Trp Gly Gln Tyr Gly Phe Asn Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Ser Arg Glu Glu Lys Lys Leu Lys Ala Tyr Gln Trp 
                245                 250                 255     


Arg Ser Glu Ala Val Asp Gln Gln Leu Thr Lys Glu Glu Val Ala Tyr 
            260                 265                 270         


Val Ile Thr Glu Ile Asn Asn Asp Leu Asn Lys Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Phe Phe Asn Lys Gln Thr 
    290                 295                 300                 


Val Gly Gln Tyr Leu Tyr Gln Gln Leu Lys Gln Asn Pro His Thr Arg 
305                 310                 315                 320 


Leu Lys Gly Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Thr Ile Trp Glu Thr Gln Ala Lys Phe His Pro Glu Leu Thr Ala Ala 
            340                 345                 350         


Leu Lys Glu Glu Ile Arg Asp Val Ile Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Lys Glu 
    370                 375                 380                 


Ile Glu Val Glu Lys Asn Gly Leu Lys Ile Lys Lys Arg Ile Gly Leu 
385                 390                 395                 400 


Arg Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp 
                405                 410                 415     


Gln Val Leu Asn Asn Leu Glu Phe Arg Asn Lys Ser Ser Lys Glu Val 
            420                 425                 430         


Phe Val Pro Asp Met Thr Ala Lys Gln Gln Leu Phe Asp Glu Leu Asn 
        435                 440                 445             


Ile Lys Gly Asn Leu Leu Ala Ser Glu Ala Ile Lys Ile Leu Gly Tyr 
    450                 455                 460                 


Lys Thr Lys Asp Trp Glu Ala Asn Tyr His Thr Leu Glu Gly Asn Arg 
465                 470                 475                 480 


Thr Asn Lys Ser Leu Tyr Asp Ala Tyr Leu Lys Ile Leu Glu Leu Glu 
                485                 490                 495     


Gly Tyr Asp Glu Asp Leu Leu Lys Leu Ser Gly Ser Asp Asp Ile Asp 
            500                 505                 510         


Val Ser Lys Ile Glu Lys Ser Ala Ser Glu Ile Lys Gln Met Val His 
        515                 520                 525             


Ala Val Phe Glu Val Leu Gly Ile Asn Ser Thr Ile Leu Asp Phe Asp 
    530                 535                 540                 


Ala Glu Ala Asp Gly Lys Ala Phe Glu Gln Gln Thr Ala Tyr Arg Leu 
545                 550                 555                 560 


Trp His Leu Leu Tyr Ser Ala Glu Asp Asp Asn Lys Lys Tyr Leu Gln 
                565                 570                 575     


Glu Asp Ile Phe Ile Tyr Gly His Asp Asn Ile Gly Leu Lys Lys Lys 
            580                 585                 590         


Leu Cys Glu Arg Phe Gly Phe Arg Pro Glu His Thr Lys Ile Leu Val 
        595                 600                 605             


Asn Val Ala Phe Ser Gly Asp Tyr Gly Ser Leu Ser Ser Lys Ala Met 
    610                 615                 620                 


Arg Lys Ile Tyr Pro Phe Ile Lys Glu Leu Thr Tyr Asp Lys Ala Cys 
625                 630                 635                 640 


Arg Glu Ala Gly Tyr Arg His Ser Ala Ser Ser Leu Ser Lys Glu Glu 
                645                 650                 655     


Ile Ala Thr Arg Pro Leu Gln Lys Arg Leu Gly Leu Leu Lys Lys Asn 
            660                 665                 670         


Glu Leu Arg Ser Pro Val Val Glu Lys Ile Leu Asn Gln Met Val Asn 
        675                 680                 685             


Val Val Asn Ala Leu Ile Asp Asp Glu Asn Lys Arg Arg Lys Ala Arg 
    690                 695                 700                 


Gly Leu Ser Asp Asp Phe His Phe Asp Glu Ile Arg Ile Glu Leu Ala 
705                 710                 715                 720 


Arg Glu Leu Lys Lys Asn Ala Lys Glu Arg Ala Glu Met Thr Thr Asn 
                725                 730                 735     


Ile Asn Ala Ala Lys Gln Leu His Glu Lys Ile Ile Lys Ile Leu Gln 
            740                 745                 750         


Thr Gln Phe Gly Val Ser Asn Pro Ser Arg Asn Asp Ile Ile Arg Tyr 
        755                 760                 765             


Lys Leu Tyr Glu Glu Leu Lys Asn Asn Gly Tyr Lys Asp Leu Tyr Thr 
    770                 775                 780                 


Asn Thr Tyr Ile Ser Pro Asp Ile Leu Phe Ser Lys Gln Ile Asp Ile 
785                 790                 795                 800 


Asp His Ile Ile Pro Gln Met Ser Leu Phe Asp Asp Ser Phe Ser Asn 
                805                 810                 815     


Lys Thr Val Val Phe Arg Lys Asp Asn Gln Asp Lys Gly Asn Lys Thr 
            820                 825                 830         


Ala Tyr Asp Tyr Ile Leu Gly Lys Tyr Gly Glu Leu Ala Leu Glu Glu 
        835                 840                 845             


Tyr Ile Ser Arg Ile Thr Ser Leu Phe Glu Leu Gly Lys Lys Asn Lys 
    850                 855                 860                 


Glu Glu Gly Ile Asn Lys Ala Lys Phe Gln Lys Leu Gln Lys Arg Leu 
865                 870                 875                 880 


Ile Asp Ile Gly Asp Gly Phe Ile Glu Lys Asp Leu Arg Glu Ser Gln 
                885                 890                 895     


Tyr Ile Ala Lys Lys Ala Lys Asn Met Leu Tyr Ala Ile Cys Arg Ser 
            900                 905                 910         


Val Val Ala Thr Ser Gly Glu Ile Thr Ala Arg Leu Arg Glu Asp Trp 
        915                 920                 925             


Asp Leu Val Asn Val Met Gln Glu Ile Asn Leu Pro Lys Phe Arg Ala 
    930                 935                 940                 


Leu Gly Leu Thr Glu Met Val Glu Lys Lys Asp Gly Gly Gln Lys Glu 
945                 950                 955                 960 


Arg Ile Ile Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met 
                965                 970                 975     


Asp Ala Leu Thr Ile Ala Phe Thr Arg His Ser His Ile Gln Tyr Leu 
            980                 985                 990         


Asn His Leu Asn Ala Arg Lys Asp  Glu Ala Ser Lys Trp  His Pro Val 
        995                 1000                 1005             


Val Ser  Lys Ile Glu Glu Lys  Glu Thr Asp Leu Val  Tyr Asp Asp 
    1010                 1015                 1020             


Leu Gly  Asn Arg Lys Arg Arg  Phe Lys Ala Pro Met  Pro Asn Phe 
    1025                 1030                 1035             


Arg Glu  Val Val Lys Glu His  Leu Glu Asn Val Leu  Ile Ser His 
    1040                 1045                 1050             


Lys Ala  Lys Asn Lys Val Val  Thr Lys Asn Lys Asn  Lys Ile Lys 
    1055                 1060                 1065             


Thr Ala  Lys Gly Glu Lys Ile  Lys Thr Glu Leu Thr  Pro Arg Gly 
    1070                 1075                 1080             


Gln Leu  His Lys Glu Thr Ile  Tyr Gly Arg Tyr Arg  Tyr Tyr Glu 
    1085                 1090                 1095             


Ser Lys  Glu Glu Lys Val Gly  Pro Lys Phe Asp Ala  Glu Thr Ile 
    1100                 1105                 1110             


Gly Met  Val Val Asn Pro Gln  Tyr Arg Lys Leu Leu  Leu Glu Arg 
    1115                 1120                 1125             


Leu Thr  Thr Asn Gly Asn Asp  Pro Lys Lys Ala Phe  Gly Gly Lys 
    1130                 1135                 1140             


Asn Thr  Leu Ser Lys Asn Pro  Leu Tyr Ile Asp Glu  Ala Lys Gln 
    1145                 1150                 1155             


Glu Leu  Leu Pro Glu Lys Ile  Leu Leu Arg Trp Leu  Asn Asp Asp 
    1160                 1165                 1170             


Tyr Ser  Ile Arg Lys Asp Val  Thr Pro Glu Asn Leu  Lys Asp Val 
    1175                 1180                 1185             


Lys Thr  Ile Glu Lys Val Leu  Asp Glu Gly Ile Lys  Lys Ile Leu 
    1190                 1195                 1200             


Leu Lys  Arg Leu Val Asp Phe  Gly Asn Asp Pro Lys  Lys Ala Phe 
    1205                 1210                 1215             


Ser Asp  Leu Asp Lys Asn Pro  Ile Trp Leu Asn Glu  Ala Lys Gly 
    1220                 1225                 1230             


Ile Thr  Ile Lys Arg Val Thr  Ile Ser Gly Val Lys  Asn Ala Glu 
    1235                 1240                 1245             


Ala Leu  His Tyr Lys Lys Asp  His Phe Gly Asn Ala  Ile Leu Asn 
    1250                 1255                 1260             


Glu Asn  Gly Lys Leu Ile Pro  Ser Ser Phe Val Ser  Thr Gly Asn 
    1265                 1270                 1275             


Asn His  His Val Ala Ile Tyr  Arg Asp Ala Glu Gly  Asn Leu Gln 
    1280                 1285                 1290             


Glu Arg  Val Val Ser Leu Phe  Asp Ala Val Gln Leu  Val Asn Ala 
    1295                 1300                 1305             


Gly Glu  Ala Val Ile Asp Arg  Glu Tyr Asn Lys Gly  Ile Gly Trp 
    1310                 1315                 1320             


Gln Phe  Leu Phe Thr Met Lys  Gln Asn Glu Met Phe  Val Phe Pro 
    1325                 1330                 1335             


Asn Glu  Lys Thr Gly Phe Asp  Pro Lys Glu Ile Asp  Leu Leu Asn 
    1340                 1345                 1350             


Thr Asp  Asn Arg Lys Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln 
    1355                 1360                 1365             


Lys Ile  Ala Ser Lys Asn Tyr  Phe Phe Arg His His  Leu Glu Thr 
    1370                 1375                 1380             


Asn Val  Glu Glu Arg Lys Glu  Leu Lys Asp Ile Val  Tyr Lys Pro 
    1385                 1390                 1395             


Gln Leu  Gly Leu Asn Ala Ile  Lys Asp Val Val Lys  Val Arg Ile 
    1400                 1405                 1410             


Asn His  Leu Gly Lys Ile Ile  Ser Val Gly Glu Tyr  
    1415                 1420                 1425 


<210>  1221
<211>  1426
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1221

Met Pro Arg Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile 
1               5                   10                  15      


Gly Phe Ala Lys Ile Ile Glu Asp Asp Asp Tyr Gln Cys Ser Ser Val 
            20                  25                  30          


Glu Leu Ile Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Gln Thr 
        35                  40                  45              


Asn Phe Glu Lys Gly Lys Pro Val Ser Val Asn Ala Asp Arg Thr Leu 
    50                  55                  60                  


Lys Arg Gly Met Arg Arg Asn Leu Asp Arg Tyr Gln Leu Arg Arg Asp 
65                  70                  75                  80  


Tyr Leu Ile Glu Ile Leu Arg Thr His His Ile Ile Thr Ala Asp Thr 
                85                  90                  95      


Lys Leu Ala Glu Asp Gly Lys Asn Thr Thr His Thr Thr Tyr Ala Leu 
            100                 105                 110         


Arg Ala Leu Ala Ala Thr Ala Glu Ile Pro Leu Glu Ala Phe Val Arg 
        115                 120                 125             


Val Leu Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys 
    130                 135                 140                 


Ala Lys Asn Glu Asp Glu Gly Gln Leu Ile Asp Gly Met Ala Ile Ala 
145                 150                 155                 160 


Arg Arg Leu Tyr Glu Glu Ser Leu Thr Pro Gly Gln Phe Ser Tyr Gln 
                165                 170                 175     


Leu Leu Lys Glu Gly Lys Lys Tyr Leu Pro Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         


Leu Asn Thr Glu Leu Asn Lys Val Trp Asn Phe Gln Lys Gln Phe Tyr 
        195                 200                 205             


Pro Glu Ile Leu Thr Asp Glu Phe Lys Arg His Leu Glu Gly Lys Gly 
    210                 215                 220                 


Gln Arg Ala Thr Ala Val Ala Phe Trp Gly Gln His Gly Phe Ser Met 
225                 230                 235                 240 


Ala Glu Asn Lys Gly Ser Arg Glu Glu Lys Lys Leu His Ala Tyr Lys 
                245                 250                 255     


Trp Arg Ser Glu Ala Ala His Lys Gln Leu Thr Lys Glu Glu Val Ala 
            260                 265                 270         


Phe Val Ile Thr Glu Ile Asn Asn Asp Leu Asn Lys Ser Ser Gly Tyr 
        275                 280                 285             


Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Phe Phe Asn Lys Gln 
    290                 295                 300                 


Thr Val Gly Gln Tyr Leu His Gln Gln Leu Lys Glu Asn Pro His Thr 
305                 310                 315                 320 


Arg Leu Lys Gly Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe 
                325                 330                 335     


Glu Thr Ile Trp Glu Met Gln Ala Lys Phe Tyr Ser Ala Leu Thr Leu 
            340                 345                 350         


Glu Leu Lys Glu Glu Val Arg Asp Val Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Met Gly Leu Ile Ser Leu Cys Glu Phe Glu Ser Lys 
    370                 375                 380                 


Glu Ile Glu Ile Glu Lys Asn Gly Glu Lys Ala Lys Lys Arg Ile Gly 
385                 390                 395                 400 


Leu Arg Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile 
                405                 410                 415     


Trp Gln Val Leu Asn Asn Leu Glu Phe Arg Asn Lys Lys Thr Lys Glu 
            420                 425                 430         


Val Phe Val Pro Glu Met Ala Ala Lys Gln Gln Leu Phe Asp Glu Leu 
        435                 440                 445             


Asn Ile Lys Gly Asn Leu Leu Ala Ser Glu Ala Ile Lys Ile Leu Gly 
    450                 455                 460                 


Tyr Lys Thr Lys Asp Trp Glu Ala Asn Tyr His Thr Leu Glu Gly Asn 
465                 470                 475                 480 


Arg Thr Asn Lys Ser Leu Tyr Asp Ala Trp Leu Lys Ile Leu Glu Leu 
                485                 490                 495     


Glu Gly Tyr Asp Glu Asn Leu Leu Lys Leu Ser Gly Ser Asp Asp Ile 
            500                 505                 510         


Asp Val Ser Lys Ile Glu Lys Pro Ala Leu Glu Ile Arg Gln Met Val 
        515                 520                 525             


Leu Ala Val Phe Glu Val Leu Gly Ile Ser Gly Ala Ile Leu Asp Phe 
    530                 535                 540                 


Asp Ala Glu Ala Asp Gly Lys Ala Phe Glu Gln Gln Pro Ala Tyr Gln 
545                 550                 555                 560 


Leu Trp His Leu Leu Tyr Ser Ala Glu Asp Asp Gly Lys Lys Tyr Ser 
                565                 570                 575     


Gln Glu Asp Ile Phe Thr Tyr Gly His Asp Asn Ile Gly Leu Lys Lys 
            580                 585                 590         


Lys Leu Cys Glu Lys Phe Gly Phe Arg Pro Glu His Thr Lys Ile Leu 
        595                 600                 605             


Val Asn Val Val Phe Ser Asp Asp Tyr Gly Asn Leu Ser Ser Lys Ala 
    610                 615                 620                 


Met Arg Arg Ile Tyr Pro Phe Ile Lys Glu Leu Thr Tyr Asp Lys Ala 
625                 630                 635                 640 


Cys Ala Glu Ala Gly Tyr Arg His Ser Ala Ala Ser Leu Thr Lys Glu 
                645                 650                 655     


Glu Ile Ala Ala Arg Pro Leu Gln Asn Arg Leu Asp Leu Leu Lys Lys 
            660                 665                 670         


Asn Glu Leu Arg Ser Pro Val Val Glu Lys Ile Leu Asn Gln Met Val 
        675                 680                 685             


Asn Val Val Asn Thr Leu Val Asp Asp Glu Asn Gln Asn Arg Lys Ala 
    690                 695                 700                 


Arg Gly Leu Ser Glu Asp Phe His Phe Asp Glu Ile Arg Ile Glu Leu 
705                 710                 715                 720 


Ala Arg Glu Leu Lys Lys Asn Ala Lys Glu Arg Ala Glu Met Thr Thr 
                725                 730                 735     


Asn Ile Asn Thr Ala Lys Gln Ser His Glu Lys Ile Ile Arg Ile Leu 
            740                 745                 750         


Gln Thr Glu Phe Gly Val Pro Asn Pro Ser Arg Asn Asp Ile Val Arg 
        755                 760                 765             


Tyr Lys Leu Tyr Glu Glu Leu Lys Asn Asn Gly Tyr Lys Asp Leu Tyr 
    770                 775                 780                 


Thr Asn Thr Tyr Ile Ser Arg Asp Ile Leu Phe Ser Lys Gln Ile Asp 
785                 790                 795                 800 


Val Asp His Ile Ile Pro Gln Met Ser Leu Phe Asp Asp Ser Phe Ser 
                805                 810                 815     


Asn Lys Thr Val Val Phe Arg Lys Asp Asn Gln Asp Lys Gly Asn Lys 
            820                 825                 830         


Thr Ala Tyr Asp Tyr Ile Leu Gly Lys Tyr Gly Glu Gln Ala Leu Glu 
        835                 840                 845             


Glu Tyr Ile Ser Arg Ile Thr Ser Leu Phe Glu Leu Gly Lys Lys Asn 
    850                 855                 860                 


Lys Glu Glu Gly Ile Gly Lys Ala Lys Phe Gln Lys Leu Gln Lys Arg 
865                 870                 875                 880 


Leu Val Asp Ile Gly Asp Gly Phe Ile Glu Arg Asp Leu Arg Glu Ser 
                885                 890                 895     


Gln Tyr Ile Ala Lys Lys Ala Asn Asn Met Leu Tyr Ala Ile Cys Arg 
            900                 905                 910         


Ser Val Val Ser Thr Ser Gly Glu Ile Thr Ala Arg Leu Arg Glu Asp 
        915                 920                 925             


Trp Asp Leu Val Asn Val Met Gln Glu Ile Asn Leu Pro Lys Phe Arg 
    930                 935                 940                 


Ala Leu Gly Leu Thr Glu Met Val Glu Lys Lys Asp Gly Ala Gln Lys 
945                 950                 955                 960 


Glu Arg Ile Thr Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala 
                965                 970                 975     


Met Asp Ala Leu Ala Ile Ala Phe Thr Arg His Ser His Ile Gln Tyr 
            980                 985                 990         


Leu Asn His Leu Asn Ala Arg Lys  Asp Glu Ala Asn Lys  Trp His Pro 
        995                 1000                 1005             


Val Val  Ser Lys Ile Glu Glu  Lys Glu Thr Val Leu  Ile Tyr Asp 
    1010                 1015                 1020             


Asp Leu  Gly Asn Arg Lys Arg  Lys Phe Lys Ala Pro  Met Pro Asn 
    1025                 1030                 1035             


Phe Arg  Glu Val Val Lys Glu  His Leu Glu Asn Val  Leu Ile Ser 
    1040                 1045                 1050             


His Lys  Ala Lys Asn Lys Val  Val Thr Lys Asn Lys  Asn Lys Ile 
    1055                 1060                 1065             


Lys Thr  Val Arg Gly Glu Lys  Val Lys Thr Glu Leu  Thr Pro Arg 
    1070                 1075                 1080             


Gly Gln  Leu His Lys Glu Thr  Ile Tyr Gly Arg Tyr  Arg Tyr Tyr 
    1085                 1090                 1095             


Glu Ser  Lys Glu Glu Lys Ile  Gly Pro Lys Phe Asp  Ala Glu Met 
    1100                 1105                 1110             


Ile Gly  Met Val Thr Asn Pro  Gln Tyr Lys Lys Leu  Leu Leu Glu 
    1115                 1120                 1125             


Arg Leu  Ala Ala Asn Gly Asn  Asp Pro Lys Lys Ala  Phe Trp Gly 
    1130                 1135                 1140             


Lys Asn  Ala Leu Asn Lys Asn  Pro Leu Tyr Val Asp  Glu Ala Arg 
    1145                 1150                 1155             


Gln Glu  Leu Leu Pro Glu Lys  Ile Leu Leu Arg Trp  Leu Asn Asp 
    1160                 1165                 1170             


Asp Tyr  Ser Ile Arg Lys Asp  Val Thr Pro Glu Asn  Leu Lys Asp 
    1175                 1180                 1185             


Ile Lys  Thr Ile Glu Lys Ile  Leu Asp Lys Gly Val  Lys Lys Ile 
    1190                 1195                 1200             


Leu Leu  Lys Arg Leu Ala Asp  Phe Gly Asn Asp Pro  Lys Lys Ala 
    1205                 1210                 1215             


Phe Ser  Asp Leu Asp Lys Asn  Pro Ile Trp Gln Asn  Glu Ala Lys 
    1220                 1225                 1230             


Arg Ile  Ala Ile Lys Arg Val  Thr Ile Ser Gly Ile  Lys Asn Ala 
    1235                 1240                 1245             


Ala Pro  Leu His His Lys Lys  Asp His Leu Gly Asn  Asp Ile Leu 
    1250                 1255                 1260             


Asp Glu  Lys Gly Lys Gln Ile  Pro Ser Ser Phe Val  Ser Thr Gly 
    1265                 1270                 1275             


Asn Asn  His His Val Ala Ile  Tyr Arg Asp Ala Glu  Gly Asn Leu 
    1280                 1285                 1290             


Gln Glu  Arg Val Val Ser Leu  Phe Asp Ala Val Gln  Leu Val Asn 
    1295                 1300                 1305             


Gly Gly  Glu Pro Val Ile Asp  Lys Glu Tyr Asn Lys  Gly Ile Gly 
    1310                 1315                 1320             


Trp Gln  Phe Leu Phe Thr Met  Lys Gln Asn Glu Met  Phe Val Phe 
    1325                 1330                 1335             


Pro Asp  Glu Lys Thr Gly Phe  Asn Pro Arg Glu Val  Asp Leu Leu 
    1340                 1345                 1350             


Ser Ser  Gly Asn Arg Lys Leu  Ile Ser Pro Asn Leu  Phe Arg Val 
    1355                 1360                 1365             


Gln Lys  Leu Ala Ser Lys Asn  Tyr Phe Phe Arg His  His Leu Glu 
    1370                 1375                 1380             


Thr Ser  Val Glu Glu Lys Lys  Glu Leu Lys Asn Ile  Ala Tyr Lys 
    1385                 1390                 1395             


Pro Gln  Leu Gly Leu Asn Ala  Leu Ser Asn Ile Val  Lys Val Arg 
    1400                 1405                 1410             


Ile Asn  His Ile Gly Glu Ile  Val Ser Val Gly Glu  Tyr 
    1415                 1420                 1425     


<210>  1222
<211>  1426
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1222

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Ile Thr Ser Ile Ser Phe 
1               5                   10                  15      


Ala His Ile Val Glu Asp Glu Asn Lys Glu Lys Ser Glu Ile Lys Glu 
            20                  25                  30          


Leu Gly Val Arg Ile Val Ser Leu Thr Thr Asp Glu Gln Ser Asp Phe 
        35                  40                  45              


Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asn Arg Thr Leu Lys His 
    50                  55                  60                  


Gly Ala Arg Leu Asn Leu Asp Arg Tyr Gln Gln Arg Arg Lys Tyr Leu 
65                  70                  75                  80  


Ile Asp Leu Leu Gln Lys Ala Asn Leu Ile Thr Ser Ser Ser Ile Leu 
                85                  90                  95      


Ala Glu Asn Gly Lys Asn Thr Thr His Ser Thr Trp Gln Leu Arg Ala 
            100                 105                 110         


Lys Ala Val Thr Glu Arg Ile Glu Lys Glu Glu Phe Ala Arg Val Leu 
        115                 120                 125             


Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala Lys 
    130                 135                 140                 


Thr Glu Asp Glu Gly Gln Ala Ile Asp Gly Met Ala Ile Ala Lys Arg 
145                 150                 155                 160 


Leu Tyr Asp Glu Asn Leu Thr Pro Gly Gln Leu Ser Leu Gln Leu Leu 
                165                 170                 175     


Gln Gln Asn Lys Lys Leu Leu Pro Asp Phe Tyr Arg Ser Asp Leu Gln 
            180                 185                 190         


Arg Glu Phe Asp Leu Val Trp Asn Phe Gln Lys Gln Phe Tyr Pro Asp 
        195                 200                 205             


Ile Leu Thr Asp Ser Phe Tyr Lys Glu Leu Gln Gly Lys Gly Lys Asp 
    210                 215                 220                 


Ala Thr Ser Lys Ala Phe Ser Lys Arg Tyr His Phe Asp Thr Ala Glu 
225                 230                 235                 240 


Asn Lys Gly Ser Lys Glu Ser Val Arg Leu Gln Ala Tyr Gln Trp Arg 
                245                 250                 255     


Ala Glu Ala Ile Ser Lys Gln Leu Ser Lys Glu Glu Val Ala Tyr Val 
            260                 265                 270         


Leu Thr Glu Ile Asn Asn Asn Leu Asn Asn Ala Ser Gly Tyr Leu Gly 
        275                 280                 285             


Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Arg Gln Thr Val 
    290                 295                 300                 


Gly Gln Tyr Leu Tyr Ala Gln Leu Gln Glu Asn Arg His Asn Ser Leu 
305                 310                 315                 320 


Lys Asn Lys Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Arg 
                325                 330                 335     


Ile Trp Glu Thr Gln Ala Ser Phe His Lys Glu Leu Thr Asp Glu Leu 
            340                 345                 350         


Lys Lys Gln Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Leu Lys 
        355                 360                 365             


Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Lys Glu Ile 
    370                 375                 380                 


Glu Ile Glu Lys Asp Gly Lys Thr Ile Thr Lys Thr Ile Gly Ala Arg 
385                 390                 395                 400 


Val Val Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln 
                405                 410                 415     


Ile Leu Asn Asn Val Thr Cys Lys Arg Lys Gly Ile Arg Lys Lys Lys 
            420                 425                 430         


Ser Ser Ala Lys Ser Thr Glu Leu Asp Leu Phe Asn Glu Pro Ser Gln 
        435                 440                 445             


Thr Ile Phe Ser Leu Asp Met Glu Cys Lys Gln Leu Leu Phe Asp Glu 
    450                 455                 460                 


Leu Asn Leu Lys Gly Asp Leu Lys Ser Asp Lys Val Leu Lys Leu Leu 
465                 470                 475                 480 


Gly Tyr Ser Pro Gln Glu Trp Glu Ile Asn Tyr Asn Gln Leu Glu Gly 
                485                 490                 495     


Asn Arg Thr Gln Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Val Glu 
            500                 505                 510         


Met Glu Ala His Asp Val Lys Asp Ile Leu Gln Ile Lys Ser Ala Lys 
        515                 520                 525             


Asp Asp Trp Ser Leu Asp Glu Ser Pro Leu Ser Ala Ser Glu Ile Lys 
    530                 535                 540                 


Glu Lys Val Lys Asp Ile Phe Gln Ala Leu Gly Ile Cys Thr Lys Ile 
545                 550                 555                 560 


Leu Tyr Phe Asp Pro Leu Leu Pro Val Lys Glu Phe Glu Lys Gln Asp 
                565                 570                 575     


Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asp Ser 
            580                 585                 590         


Thr Ser Gly Asn Glu Thr Leu Tyr Arg Ile Leu Glu Lys Lys Tyr Ala 
        595                 600                 605             


Phe Lys Arg Glu His Ala Arg Ile Leu Ala Asn Val Ala Leu Gln Asp 
    610                 615                 620                 


Asp Tyr Gly Ser Leu Ser Thr Lys Ala Ile Arg Lys Ile Tyr Pro Asn 
625                 630                 635                 640 


Ile Lys Glu Asn Gln Tyr Ser Lys Ala Cys Glu Leu Ala Gly Tyr Lys 
                645                 650                 655     


His Ser Lys Leu Ser Leu Thr Thr Glu Glu Leu Glu Ala Arg Glu Leu 
            660                 665                 670         


Lys Asn Ile Ile Pro Leu Leu Lys Lys Asn Ala Leu Arg Asn Pro Val 
        675                 680                 685             


Val Glu Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Ala Leu Ile 
    690                 695                 700                 


Glu Lys Asn Ser Glu Arg Asp Ala Glu Gly Lys Ile Thr Lys Tyr Phe 
705                 710                 715                 720 


His Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn 
                725                 730                 735     


Ala Gln Lys Arg Tyr Glu Met Thr Gln Asn Ile Asn Lys Ala Lys Leu 
            740                 745                 750         


Glu His Gln Lys Ile Ser Glu Ile Leu Gln Lys Lys Phe Gly Ile Lys 
        755                 760                 765             


Asn Pro Thr Lys Ser Asp Ile Ile Arg Tyr Arg Leu Tyr Gln Glu Leu 
    770                 775                 780                 


Glu His Asn Gly Tyr Lys Glu Leu Tyr Thr Asn Ala Pro Ile Ala Arg 
785                 790                 795                 800 


Asp Met Leu Phe Ser Lys Asn Ile Glu Ile Glu His Ile Val Pro Lys 
                805                 810                 815     


Ala Arg Val Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Thr Phe His 
            820                 825                 830         


Arg Ile Asn Ser Asn Lys Gly Glu Tyr Thr Ala Phe Asp Tyr Ile Thr 
        835                 840                 845             


Ser Leu Asn Ser Glu Glu Glu Leu Asn Gln Tyr Leu Thr Arg Val Glu 
    850                 855                 860                 


Asn Ala Tyr Lys Thr Lys Ser Ile Ser Leu Thr Lys Tyr Lys Asn Leu 
865                 870                 875                 880 


Leu Lys Lys Ala Ser Glu Ile Gly Asp Gly Phe Ile Asn Arg Asp Leu 
                885                 890                 895     


Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Phe Gln 
            900                 905                 910         


Val Thr Lys Asn Val Leu Ser Thr Ser Gly Ser Ile Thr Asp Arg Leu 
        915                 920                 925             


Arg Glu Asp Trp Gly Leu Val Asp Val Met Lys Glu Leu Asn Met Pro 
    930                 935                 940                 


Lys Tyr Gln Ser Leu Gly Leu Thr Glu Val Glu Glu Arg Lys Glu Gly 
945                 950                 955                 960 


Asn Lys Val Thr Val Ile Lys Asn Trp Thr Lys Arg Asn Asp His Arg 
                965                 970                 975     


His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Pro Ser Tyr 
            980                 985                 990         


Ile Gln Tyr Leu Asn His Leu Asn  Ala Arg Lys Asp Glu  Asn Asn Lys 
        995                 1000                 1005             


Asn Tyr  Ser Val Ile Leu Ala  Ile Glu Glu Lys Glu  Thr Ile Lys 
    1010                 1015                 1020             


Val Pro  Thr Asn Asn Gly Lys  Asn Lys Arg Val Phe  Ile Glu Pro 
    1025                 1030                 1035             


Ile Pro  Asn Phe Arg Gln Val  Ala Lys Lys His Leu  Glu Glu Ile 
    1040                 1045                 1050             


Phe Ile  Ser His Lys Ala Lys  Asn Lys Val Val Thr  Lys Asn Val 
    1055                 1060                 1065             


Asn Lys  Pro Ala Gly Thr Asp  Lys Gln Gln Ile Thr  Leu Thr Pro 
    1070                 1075                 1080             


Arg Gly  Gln Leu His Lys Glu  Thr Ile Tyr Gly Lys  Tyr Gln Tyr 
    1085                 1090                 1095             


Tyr Val  Asn Lys Glu Glu Lys  Val Gly Val Lys Phe  Asp Glu Arg 
    1100                 1105                 1110             


Thr Ile  Thr Lys Val Ser Asn  Pro Leu Tyr Arg Glu  Ala Leu Leu 
    1115                 1120                 1125             


Lys Arg  Leu Gln Ala Asn Asp  Asn Asp Pro Lys Lys  Ala Phe Thr 
    1130                 1135                 1140             


Gly Lys  Asn Ala Leu Ser Lys  Asn Pro Ile Tyr Leu  Asp Glu Thr 
    1145                 1150                 1155             


Lys Thr  Lys Thr Leu Pro Glu  Lys Val Asn Leu Thr  Tyr Leu Glu 
    1160                 1165                 1170             


Glu Asp  Phe Ser Ile Arg Lys  Asp Ile Ser Pro Asp  Asn Phe Lys 
    1175                 1180                 1185             


Asp Leu  Lys Ser Ile Glu Lys  Val Ile Asp Gln Gly  Val Lys Arg 
    1190                 1195                 1200             


Ile Leu  Ile Lys Arg Leu Gln  Asp Tyr Asp Asn Asp  Pro Lys Lys 
    1205                 1210                 1215             


Ala Phe  Val Asp Leu Glu Lys  Asn Pro Ile Trp Leu  Asn Lys Glu 
    1220                 1225                 1230             


Lys Gly  Ile Ala Ile Lys Arg  Val Thr Ile Ser Gly  Val Ser Asn 
    1235                 1240                 1245             


Ala Gln  Pro Leu His Ile Gly  Lys Asp His Leu Gly  Lys Thr Thr 
    1250                 1255                 1260             


Leu Asn  Lys Glu Gly Asn Glu  Ile Pro Val Asp Tyr  Val Ser Thr 
    1265                 1270                 1275             


Gly Asn  Asn His His Val Ala  Ile Tyr Arg Asp Lys  Glu Gly Asn 
    1280                 1285                 1290             


Leu Gln  Glu Gln Ile Val Ser  Phe Phe Glu Ala Val  Val Arg Thr 
    1295                 1300                 1305             


Gln Gln  Gly Leu Pro Ile Ile  Asp Lys Thr Tyr Lys  Gln Glu Glu 
    1310                 1315                 1320             


Gly Trp  Gln Phe Leu Phe Thr  Met Lys Gln Asn Glu  Met Phe Val 
    1325                 1330                 1335             


Phe Pro  Asn Ala Thr Thr Gly  Phe Asn Pro Ala Glu  Ile Asp Leu 
    1340                 1345                 1350             


Leu Asp  Pro Lys Asn Lys Lys  Leu Ile Ser Pro Asn  Leu Phe Arg 
    1355                 1360                 1365             


Val Gln  Lys Ile Ala Thr Lys  Asp Tyr Phe Phe Arg  His His Leu 
    1370                 1375                 1380             


Glu Thr  Asn Val Glu Thr Asp  Asn Val Leu Lys Asn  Thr Thr Trp 
    1385                 1390                 1395             


Lys Arg  Glu Gly Leu Ser Gly  Leu Lys Asp Ile Val  Lys Val Arg 
    1400                 1405                 1410             


Ile Asn  His Leu Gly Asp Ile  Val Ser Ile Gly Glu  Tyr 
    1415                 1420                 1425     


<210>  1223
<211>  1426
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1223

Met Lys His Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Lys Gln Asp Phe Glu Asn Lys Gln Gly Glu Ile Leu Gly 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Asp Phe 
        35                  40                  45              


Gly Lys Gly Asn Ser Val Ser Gln Thr Ala Glu Arg Thr Lys Tyr Arg 
    50                  55                  60                  


Ser Val Arg Arg Leu Arg Glu Arg Phe Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Ile Leu Asn Val Leu Gly Phe Leu Pro Glu His Tyr Ala Ser 
                85                  90                  95      


Gln Ile Asp Phe Thr Lys Arg Phe Gly Lys Phe Lys Glu Glu Thr Glu 
            100                 105                 110         


Pro Lys Leu Val Tyr Ser Asn Glu Gly Phe Ile Phe Lys Lys Ser Phe 
        115                 120                 125             


Glu Glu Met Leu Ser Asp Phe Lys Thr His Gln Pro Gln Leu Leu Glu 
    130                 135                 140                 


Asp Asn Lys Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys 
145                 150                 155                 160 


Lys Ala Leu Ser Arg Lys Ile Glu Lys Glu Glu Leu Ala Trp Ile Leu 
                165                 170                 175     


Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu 
            180                 185                 190         


Glu Glu Glu Asn Pro Asn Lys Leu Ile Glu Phe Tyr Ser Leu Lys Ile 
        195                 200                 205             


Val Asp Val Leu Ala Asp Glu Pro Gln Lys Gly Lys Ser Asp Ile Trp 
    210                 215                 220                 


Tyr Ser Leu Ile Leu Glu Asn Gly Trp Ile Tyr Arg Arg Ser Ser Lys 
225                 230                 235                 240 


Thr Pro Leu Phe Asp Trp Lys Glu Lys Thr Arg Asp Phe Ile Val Thr 
                245                 250                 255     


Ile Asp Leu Asn Asp Asp Gly Ser Val Lys Thr Asp Lys Glu Gly Asn 
            260                 265                 270         


Glu Lys Arg Ser Phe Arg Ala Pro Gly Glu Asn Asp Trp Thr Leu Val 
        275                 280                 285             


Lys Lys Lys Thr Glu Gln Glu Ile Asp Ser Ser Arg Lys Thr Val Gly 
    290                 295                 300                 


Thr Tyr Ile Tyr Glu Thr Leu Leu Lys Asn Pro Lys Gln Lys Ile Lys 
305                 310                 315                 320 


Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Thr Glu Leu 
                325                 330                 335     


Lys Gln Ile Leu Glu Lys Gln Lys Glu Phe His Pro Glu Leu Arg Gln 
            340                 345                 350         


Asp Asp Leu Tyr Ser Asp Cys Val Arg Glu Leu Tyr Lys Asn Asn Glu 
        355                 360                 365             


Ala His Gln Leu Thr Leu Ser Lys Lys Asp Phe Val His Leu Phe Met 
    370                 375                 380                 


Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Gly Ser Gln Lys Ser Ser 
385                 390                 395                 400 


Ile Ser Asn Cys Thr Leu Glu Phe Arg Lys Tyr Lys Asp Glu Asn Gly 
                405                 410                 415     


Thr Glu His Thr Gln Tyr Leu Lys Ala Ile Pro Lys Ser Asn Pro Tyr 
            420                 425                 430         


Tyr Gln Glu Phe Arg Ile Trp Gln Trp Met Tyr Asn Leu Asn Ile Tyr 
        435                 440                 445             


Lys Lys Glu Asp Asp Thr Asn Val Thr Lys Asp Phe Leu Asn Thr Lys 
    450                 455                 460                 


Ala Asp Phe Glu Ser Leu Phe Glu Phe Leu Asn Asn Arg Lys Glu Val 
465                 470                 475                 480 


Asp Gln Lys Ala Leu Leu Lys His Phe Lys Leu Asn Asp Lys Thr His 
                485                 490                 495     


Arg Trp Asn Tyr Val Glu Asp Lys Lys Tyr Pro Cys Asn Glu Thr Lys 
            500                 505                 510         


Thr Met Ile Leu Ser Arg Leu Asp Lys Val Glu Asn Ile Ser Asp Asp 
        515                 520                 525             


Phe Leu Thr Arg Glu Met Glu Gln Lys Ile Trp His Ile Ile Tyr Ser 
    530                 535                 540                 


Val Asn Asp Lys Val Glu Tyr Glu Lys Ala Leu Lys Ser Phe Ala Gly 
545                 550                 555                 560 


Lys Asn Asn Leu Glu Asp Asn Ser Phe Phe Glu Ala Phe Lys Lys Phe 
                565                 570                 575     


Pro Pro Phe Lys Ser Glu Tyr Gly Ala Phe Ser Glu Lys Ala Ile Lys 
            580                 585                 590         


Lys Leu Leu Pro Leu Met Arg Met Gly Lys Phe Trp Asn Tyr Asn Asn 
        595                 600                 605             


Ile Ser Glu Gln Ser Lys Ser Arg Ile Gln Lys Ile Ile Thr Gly Glu 
    610                 615                 620                 


Tyr Asp Glu Asn Ile Lys Asp Lys Val Arg Glu Lys Ala Ile His Leu 
625                 630                 635                 640 


Thr Thr Glu Thr Asp Phe Gln Gly Leu Gln Leu Trp Leu Val Gln Tyr 
                645                 650                 655     


Ile Val Tyr Gly Arg His Ser Glu Ala Ala Met Leu Gly Lys Trp Asn 
            660                 665                 670         


Ser Ala Asp Asp Leu Glu Glu Phe Leu Lys Glu Phe Lys Gln His Ser 
        675                 680                 685             


Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val 
    690                 695                 700                 


Val Lys Asp Ile Trp Gln Lys Tyr Gly Asn Gly Ala Lys Asp Phe Phe 
705                 710                 715                 720 


Arg Glu Ile His Ile Glu Leu Gly Arg Glu Met Lys Leu Pro Ala Glu 
                725                 730                 735     


Glu Arg Glu Arg Leu Thr Arg Gln Ile Ser Glu Asn Glu Asn Thr Asn 
            740                 745                 750         


Phe Arg Ile Lys Ala Leu Leu Ala Glu Met Met Asp Asp Asn Ser Val 
        755                 760                 765             


Glu Asn Val Arg Pro Phe Ser Pro Met Gln Gln Glu Ile Leu Lys Ile 
    770                 775                 780                 


Tyr Glu Glu Gly Val Leu Asn Ser Asp Ile Glu Ile Glu Asp Asp Ile 
785                 790                 795                 800 


Leu Lys Ile Ser Lys Thr Ala Gln Pro Ser Ser Ser Asp Leu Lys Arg 
                805                 810                 815     


Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Lys Ser Pro Tyr Thr Gly Gln 
            820                 825                 830         


Ile Ile Pro Leu Asn Lys Leu Phe Thr Pro Glu Tyr Glu Ile Glu His 
        835                 840                 845             


Ile Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Thr Asn Lys Ile 
    850                 855                 860                 


Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp Asn Tyr Ile Gly Leu 
865                 870                 875                 880 


Glu Phe Ile Lys Lys Leu Gly Gly Ser Ile Val Glu Cys Gly Asn Gly 
                885                 890                 895     


Lys Ser Val Ala Ile Leu Lys His Asp Ala Tyr Glu Asp Phe Ile Lys 
            900                 905                 910         


Lys His Tyr Ala Asn Asn Arg Ser Lys Arg Asn Lys Leu Leu Leu Glu 
        915                 920                 925             


Asp Ile Pro Glu Lys Met Ile Glu Arg Gln Met Asn Asp Thr Arg Tyr 
    930                 935                 940                 


Ile Ser Lys Tyr Ile Ser Gly Val Leu Ser Asn Ile Val Arg Val Glu 
945                 950                 955                 960 


Asp Gly Thr Asp Glu Gly Val Asn Ser Lys Asn Ile Val Pro Gly Asn 
                965                 970                 975     


Gly Lys Ile Thr Thr Gln Leu Lys Gln Asp Trp Gly Leu Asn Asp Val 
            980                 985                 990         


Trp Asn Asp Leu Ile Leu Pro Arg  Phe Glu Arg Met Asn  His Leu Thr 
        995                 1000                 1005             


Asn Ser  Thr Asp Phe Thr Ala  Trp Asn Glu Asn His  Gln Lys Phe 
    1010                 1015                 1020             


Leu Pro  Thr Val Pro Val Glu  Phe Ser Lys Gly Phe  Ser Lys Lys 
    1025                 1030                 1035             


Arg Ile  Asp His Arg His His  Ala Leu Asp Ala Leu  Val Ile Ala 
    1040                 1045                 1050             


Cys Ala  Thr Lys Asp His Val  Asn Leu Leu Asn Asn  Gln Ser Ala 
    1055                 1060                 1065             


Lys Ser  Asp Thr Lys Arg Tyr  Asp Leu Lys Lys Lys  Leu Met Lys 
    1070                 1075                 1080             


Phe Glu  Lys Val Ala Tyr Asn  His Pro Gln Thr Gly  Glu Arg Ile 
    1085                 1090                 1095             


Glu Arg  Glu Ala Pro Lys Gln  Phe Leu Lys Pro Trp  Glu Thr Phe 
    1100                 1105                 1110             


Thr Ile  Asp Ala Lys Asn His  Leu Glu Thr Ile Ile  Val Ser Phe 
    1115                 1120                 1125             


Lys Gln  Asn Leu Arg Val Ile  Asn Lys Ala Thr Asn  Tyr Tyr Glu 
    1130                 1135                 1140             


Lys Tyr  Val Glu Lys Glu Gly  Thr Lys Val Lys Glu  Arg Val Glu 
    1145                 1150                 1155             


Gln Lys  Gly Thr Asn Trp Ala  Ile Arg Lys Pro Met  His Lys Glu 
    1160                 1165                 1170             


Thr Val  Ser Gly Lys Ile Asp  Leu Pro Arg Ile Lys  Val Pro Lys 
    1175                 1180                 1185             


Gly Lys  Ile Leu Thr Ala Thr  Arg Lys Ser Leu Asp  Thr Ser Phe 
    1190                 1195                 1200             


Asn Leu  Lys Thr Ile Gly Ser  Ile Thr Asp Thr Gly  Ile Gln Lys 
    1205                 1210                 1215             


Ile Leu  Lys Asn Tyr Leu Gln  Tyr Lys Asp Asn Asn  Pro Glu Leu 
    1220                 1225                 1230             


Ala Phe  Ser Pro Glu Gly Ile  Glu Asp Met Asn Lys  Asn Ile Glu 
    1235                 1240                 1245             


Lys Phe  Asn Asn Gly Lys Ala  His Gln Ser Ile Ser  Lys Val Arg 
    1250                 1255                 1260             


Val Phe  Glu Leu Gly Ser Lys  Phe Pro Leu Gly Gln  Ser Gly Asn 
    1265                 1270                 1275             


Lys Lys  Glu Lys Tyr Val Glu  Ala Ala Lys Gly Thr  Asn Leu Phe 
    1280                 1285                 1290             


Phe Ala  Ile Tyr Gln Asp Lys  Asn Gly Lys Arg Ser  Tyr Glu Thr 
    1295                 1300                 1305             


Ile Pro  Leu Asn Glu Val Ile  Glu Arg Gln Lys Gln  Gly Leu Ser 
    1310                 1315                 1320             


Val Val  Asp Leu Lys Gly Val  Asp Asp Phe Tyr Leu  Ser Pro Asn 
    1325                 1330                 1335             


Asp Leu  Val Tyr Val Pro Ser  Glu Asp Glu Leu Glu  Asn Ile Ser 
    1340                 1345                 1350             


Met Ile  Asp Phe Asn Lys Met  Ser Ser Glu Arg Ile  Tyr Lys Val 
    1355                 1360                 1365             


Val Ser  Phe Thr Gly Asn Gln  Ile Phe Phe Val Arg  Gln Asp Ile 
    1370                 1375                 1380             


Ala Thr  Ser Val Val Asn Lys  Ala Glu Phe Ser Thr  Leu Asn Lys 
    1385                 1390                 1395             


Met Glu  Arg Ala Ile Asp Gly  Thr Met Ile Lys Glu  Thr Cys Val 
    1400                 1405                 1410             


Lys Leu  Gln Ile Asp Arg Leu  Gly Asn Ile Ser Lys  Ile 
    1415                 1420                 1425     


<210>  1224
<211>  1426
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1224

Met Tyr Asn Ile Gly Leu Asp Ile Gly Thr Gly Ser Val Gly Trp Cys 
1               5                   10                  15      


Leu Thr Asp Glu Asn Gly Tyr Leu Leu Lys Val Asn Arg Lys Gly Asn 
            20                  25                  30          


Asn Gly Asn Thr Tyr Arg Asn Ser Ala Trp Gly Val Arg Leu Phe Glu 
        35                  40                  45              


Ser Ala Asp Thr Ala Ala Asp Cys Arg Ile Lys Arg Ser Thr Arg Arg 
    50                  55                  60                  


Arg Tyr Lys Arg Arg Arg Thr Arg Ile Ile Glu Leu Arg Lys Ile Met 
65                  70                  75                  80  


Ser Asp Met Ile Met Pro Ile Asp Pro Asn Phe Tyr Ala Arg Leu Asp 
                85                  90                  95      


Glu Ala Phe Leu Trp Asn Glu Asp Lys Ser Asp Lys Ala Lys Ala Pro 
            100                 105                 110         


Phe Leu Leu Phe Asn Asp Asn Gly Tyr Asp Asp Val Lys Tyr Tyr Thr 
        115                 120                 125             


Asp Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Leu Glu Thr Lys 
    130                 135                 140                 


Glu Lys Ala Asp Pro Arg Phe Ile Tyr Leu Ala Leu His His Met Met 
145                 150                 155                 160 


Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Gln Ser Phe Glu Ala Ile 
                165                 170                 175     


Asp Asn Ile Glu Asp Thr Phe Ile Glu Leu Glu His Leu Val Asn Val 
            180                 185                 190         


Tyr Val Lys Glu Lys Glu Asp Thr Asp Asn Asn Ala Glu Asn Asn Ala 
        195                 200                 205             


Leu Tyr Gln Glu Met Lys Asn Tyr Leu Ala Asp Asn Lys Val Lys Asn 
    210                 215                 220                 


Lys Asp Lys Lys Glu Tyr Ile Thr Asp Thr Phe Ile Lys Ala Asp Tyr 
225                 230                 235                 240 


Asp Asn Lys Tyr Ser Lys Glu Leu Ala Ala Ala Val Leu Gly Tyr Glu 
                245                 250                 255     


Phe Asn Val Gly Ile Ile Val Asn Asp Asn Ser Leu Thr Asp Glu Asp 
            260                 265                 270         


Gly Lys Ala Leu Lys Ala Lys Phe Ala Asp Ala Lys Tyr Glu Glu Lys 
        275                 280                 285             


Glu Glu Lys Leu Ser Asp Thr Leu Gly Glu Arg Tyr Tyr Ile Ile Glu 
    290                 295                 300                 


Thr Leu Lys Lys Ile Tyr Ser Trp Lys Val Leu His Ser Ile Leu Gly 
305                 310                 315                 320 


Asp Asn Lys Tyr Leu Ser Tyr Ala Met Val Asp Lys Tyr Glu Lys His 
                325                 330                 335     


Ser Glu Gln Leu Lys Ala Leu Lys Tyr Leu Phe His Lys Tyr Thr Ser 
            340                 345                 350         


Gln Asp Glu Tyr Ser Glu Phe Phe His Gln Glu Lys Asn Lys Glu Gly 
        355                 360                 365             


Lys Tyr Ile Val Asn Tyr Ala Asn Tyr Ile Lys Gly Ile Lys Arg Leu 
    370                 375                 380                 


Ser Asn Glu Thr Asn Lys Lys Tyr Asn Thr Lys Gln Gln Leu Tyr Gln 
385                 390                 395                 400 


Ser Ile Met Lys Ile Leu Gly Glu Arg Ala Ala Asp Asp Glu Val Tyr 
                405                 410                 415     


Lys Lys Ile Leu Val Glu Met Glu Gln Glu Thr Phe Leu Glu Lys Ile 
            420                 425                 430         


Asn Asn Val Asp Asn Ser Ala Ile Pro Tyr Gln Leu Asn Leu Met Glu 
        435                 440                 445             


Met Asp Lys Ile Leu Thr Gln Gln Gly Val Tyr Tyr Lys Glu Leu Arg 
    450                 455                 460                 


Asp Asn Lys Glu Leu Leu Leu Lys Met Leu Thr Ser Lys Ile Pro Tyr 
465                 470                 475                 480 


Tyr Val Gly Pro Leu Asn Asn Asn Ser Asn Gly Asn Arg Asn Phe Ala 
                485                 490                 495     


Trp Met Thr Lys Lys Asp Gly Lys Glu Asn Glu Lys Val Tyr Pro Trp 
            500                 505                 510         


Asn Val Lys Asp Val Val Asp Ile Asp Val Thr Ala Glu Asp Phe Ile 
        515                 520                 525             


Thr Arg Met Thr Asn Tyr Cys Thr Tyr Leu Pro Asn Glu Lys Val Leu 
    530                 535                 540                 


Pro Lys Glu Ser Leu Leu Tyr Gln Arg Tyr Met Leu Leu Glu Glu Leu 
545                 550                 555                 560 


Ser Gln Ile Arg Ile Asp Gly Arg Lys Leu Ser Lys Glu Asp Arg Lys 
                565                 570                 575     


Ala Ile Ile Asn Asp Leu Phe Ile Gly Lys Gly Arg Val Lys Val Ser 
            580                 585                 590         


Asp Lys Asp Phe Lys Glu Tyr Leu Asn Lys Val Asn Tyr Val Lys Val 
        595                 600                 605             


Asn Gly Lys Gly Tyr Asp Val Thr Gly Tyr Gln Ser Asp Asp Gly Phe 
    610                 615                 620                 


Ala Cys Ala Leu Ser Ser Tyr Asn Lys Phe Arg Arg Ile Leu Gly His 
625                 630                 635                 640 


Ile Asp Glu Gln Asn Glu Lys Met Ile Glu Asp Ile Ile Tyr Trp Ile 
                645                 650                 655     


Thr Val Phe Glu Asp Lys Asp Ile Val Lys Arg Lys Ile Asn Lys Gln 
            660                 665                 670         


Tyr Ala Asp Arg Leu Ser Ala Glu Gln Leu Lys Lys Ile Leu Lys Leu 
        675                 680                 685             


Lys Phe Lys Gly Trp Gly Arg Tyr Ser Ser Lys Leu Leu Asn Gly Ile 
    690                 695                 700                 


Lys Gly Asp Met Gly Thr Thr Ile Ile Glu Met Leu Glu Asp Ala Asp 
705                 710                 715                 720 


Glu Arg Phe Ala Tyr Cys Asp Tyr Cys Pro Asn Phe Met Gln Ile Ile 
                725                 730                 735     


Asn Lys Asp Glu Lys Ile Lys Gln Ile Ile Asp Asp Asn Arg Pro Arg 
            740                 745                 750         


Tyr Asp Gly Thr Lys Asp Leu Leu Asp Val Ile Gln Asp Met His Thr 
        755                 760                 765             


Ser Pro Ala Asn Arg Arg Gly Ile Trp Gln Thr Met Lys Ala Ile Glu 
    770                 775                 780                 


Glu Ile Ile Glu Tyr Met Gly Glu Lys Pro Gln Gln Ile Tyr Ile Glu 
785                 790                 795                 800 


Phe Ala Arg Glu Asp Asp Pro Lys Ala Lys Asn Lys Arg Ala Asp Ser 
                805                 810                 815     


Arg Lys Arg Ala Ile Asp Lys Ala Leu Lys Lys Leu Lys Glu Asp Val 
            820                 825                 830         


Val Asp Glu Tyr Asn Glu Lys Val Tyr Lys Glu Leu Lys Gln Tyr Glu 
        835                 840                 845             


Lys Arg Leu Asp Glu Glu Lys Val Tyr Leu Tyr Phe Met Gln Asn Gly 
    850                 855                 860                 


Lys Asp Leu Tyr Phe Pro Asp Lys Glu Leu Asn Leu Asp Glu Leu Glu 
865                 870                 875                 880 


Lys Leu Glu Ile Asp His Ile Ile Pro Tyr Ser Leu Ser Asp Asp Asp 
                885                 890                 895     


Ser Leu Asp Asn Lys Ala Leu Val Leu Lys Gly Asn Asn Gln Asn Lys 
            900                 905                 910         


Gly Asn Lys Ile Val Lys Glu Ala Phe Pro Gln Leu Phe Ser Asn Ser 
        915                 920                 925             


Gly Thr Val Asn Tyr Trp Lys Asn Leu Lys Lys Ala Gly Leu Ile Ser 
    930                 935                 940                 


Glu Lys Lys Tyr Asn Asn Leu Gln Lys Asn Asn Val Asp Asp Ile Leu 
945                 950                 955                 960 


Thr Lys Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Val 
                965                 970                 975     


Lys Ser Val Ala Asn Leu Ile Leu Asp Tyr Tyr Asn Glu Gln Ile Asp 
            980                 985                 990         


Val Ile Glu Val Lys Ala Ser Leu  Ser Thr Ser Val Arg  Lys Met Leu 
        995                 1000                 1005             


Thr Tyr  Glu Lys Lys Asp Asn  Asn Gly Phe Trp Ile  Glu Asn Lys 
    1010                 1015                 1020             


Asp Asn  Cys Met Phe Tyr Lys  Asn Arg Asp Met Asn  Asp Tyr His 
    1025                 1030                 1035             


His Ala  His Asp Ala Tyr Leu  Ala Asn Ile Ile Gly  Leu Tyr Ile 
    1040                 1045                 1050             


Gln Lys  Asn Tyr Pro Arg Leu  Gln Lys Glu Leu Asn  Tyr Ser Gln 
    1055                 1060                 1065             


Tyr Arg  Arg Ile Trp Arg Lys  Tyr Tyr Glu Asn Thr  Lys Asn Asn 
    1070                 1075                 1080             


Asn Gly  Val Asn Trp Phe Ala  Thr Leu Gly Lys Phe  Ser Ser Asn 
    1085                 1090                 1095             


Asn Glu  Glu Ala Asp Trp Tyr  Gly Gln Asp Ile Ile  Ala Tyr Met 
    1100                 1105                 1110             


Arg Lys  Ile Phe Cys Tyr Arg  Asp Val Leu Ile Ser  Lys Lys Val 
    1115                 1120                 1125             


Glu Glu  Asn Thr Gly Ala Phe  Tyr Ser Glu Thr Lys  Tyr Pro Arg 
    1130                 1135                 1140             


Glu Asp  Lys Ala Gly Ser Lys  Leu Val Pro Leu Lys  Gln Gly Asn 
    1145                 1150                 1155             


Asn Met  Arg Gly Ala Asn Asn  Leu Lys Glu Leu Asp  Ala Arg Lys 
    1160                 1165                 1170             


Tyr Gly  Gly Tyr Lys Gly Gly  Glu Lys Ala Tyr Phe  Val Leu Val 
    1175                 1180                 1185             


Lys Tyr  Cys Ile Glu Lys Ala  Leu Lys Lys Lys Val  Lys Lys Glu 
    1190                 1195                 1200             


Tyr His  Met Glu Phe Val Glu  Ile Pro Val Tyr Ile  Ala Arg Gly 
    1205                 1210                 1215             


Ile Lys  Asn Lys Asp Ile Asn  Leu Tyr Asp Tyr Val  Cys Asp Ile 
    1220                 1225                 1230             


Leu Asn  Glu Ala Asn Lys Asn  Ser Val Ala Asp Val  Glu Ile Leu 
    1235                 1240                 1245             


Arg Asn  Lys Val Pro Lys Tyr  Gln Met Ile Ile Gly  Glu Asn Gly 
    1250                 1255                 1260             


Glu Glu  Tyr Tyr Leu Val Ser  Ala Thr Glu Val Ile  Asn Ser Lys 
    1265                 1270                 1275             


Gln Phe  Val Leu Gly Gly Ala  Asn Gln Gln Tyr Asn  Arg Leu Leu 
    1280                 1285                 1290             


Asn Tyr  Ile Lys Tyr Gly Lys  Asn Asp Lys Trp Gln  Tyr Ile Gln 
    1295                 1300                 1305             


Thr Glu  Leu Leu Asp Glu Gln  Leu Ile Gly Leu Tyr  Asn Leu Leu 
    1310                 1315                 1320             


Ile Ala  Lys Ile Lys Asp Glu  Tyr Lys Gly Phe Ser  Lys Glu Ala 
    1325                 1330                 1335             


Val Arg  Ile Gln Glu Asn Asn  Ser Phe Asp Lys Leu  Asp Val Lys 
    1340                 1345                 1350             


Asp Lys  Lys Glu Phe Ile Ala  Glu Met Ile Lys Leu  Val Gln Pro 
    1355                 1360                 1365             


Asp Ser  Asp Tyr Pro Tyr Leu  Gly Lys Tyr Ala Thr  Gly Leu Ser 
    1370                 1375                 1380             


Asn Arg  Met Gly Arg Lys Thr  Gly Glu Lys Val Gly  Lys Lys Ile 
    1385                 1390                 1395             


Thr Leu  Val Asp Lys Ser Val  Thr Gly Leu Tyr Glu  Arg Arg Thr 
    1400                 1405                 1410             


Thr Phe  Glu Leu Glu Asp Asp  Ser Ser Thr Lys Ser  Cys 
    1415                 1420                 1425     


<210>  1225
<211>  1426
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1225

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Phe 
1               5                   10                  15      


Ala His Ile Val Glu Asp Glu Asn Lys Glu Lys Ser Glu Ile Lys Glu 
            20                  25                  30          


Leu Gly Val Arg Ile Val Ser Leu Thr Thr Asp Glu Gln Ser Asp Phe 
        35                  40                  45              


Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asn Arg Thr Leu Lys His 
    50                  55                  60                  


Gly Ala Arg Leu Asn Leu Asp Arg Tyr Gln Gln Arg Arg Lys Tyr Leu 
65                  70                  75                  80  


Ile Asp Leu Leu Gln Lys Ala Asn Leu Ile Thr Pro Ser Ser Ile Leu 
                85                  90                  95      


Ala Glu Asn Gly Lys Asn Thr Thr His Ser Thr Trp Gln Leu Arg Ala 
            100                 105                 110         


Lys Ala Val Thr Glu Arg Ile Glu Lys Glu Glu Phe Ala Arg Ile Leu 
        115                 120                 125             


Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala Lys 
    130                 135                 140                 


Thr Glu Asp Glu Gly Gln Ala Ile Asp Gly Met Ala Ile Ala Lys Arg 
145                 150                 155                 160 


Leu Tyr Asp Glu Asn Leu Thr Pro Gly Gln Leu Ser Leu Gln Leu Leu 
                165                 170                 175     


Gln Gln Asn Lys Lys Leu Leu Pro Asp Phe Tyr Arg Ser Asp Leu Gln 
            180                 185                 190         


Lys Glu Phe Asp Leu Val Trp Asn Phe Gln Lys Gln Phe Tyr Pro Asp 
        195                 200                 205             


Ile Leu Thr Asp Ile Phe Tyr Lys Glu Leu Gln Gly Lys Gly Lys Asp 
    210                 215                 220                 


Ala Thr Ser Lys Ala Phe Ser Lys Arg Tyr His Phe Asp Thr Thr Glu 
225                 230                 235                 240 


Asn Lys Gly Ser Lys Glu Ser Val Arg Leu Gln Ala Tyr Gln Trp Arg 
                245                 250                 255     


Ala Glu Ala Ile Ser Lys Gln Leu Ser Lys Glu Glu Val Ala Tyr Val 
            260                 265                 270         


Leu Thr Glu Ile Asn Asn Asn Leu Asn Asn Ala Ser Gly Tyr Leu Gly 
        275                 280                 285             


Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Arg Gln Thr Val 
    290                 295                 300                 


Gly Gln Tyr Leu Tyr Ala Lys Leu Gln Glu Asn Arg His Asn Ser Leu 
305                 310                 315                 320 


Lys Asn Lys Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Arg 
                325                 330                 335     


Ile Trp Glu Thr Gln Ala Ser Phe His Lys Glu Leu Thr Asp Glu Leu 
            340                 345                 350         


Lys Lys Gln Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Pro Lys 
        355                 360                 365             


Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Lys Glu Ile 
    370                 375                 380                 


Glu Ile Glu Lys Asp Gly Lys Thr Ile Thr Lys Asn Ile Gly Ala Arg 
385                 390                 395                 400 


Val Val Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln 
                405                 410                 415     


Ile Leu Asn Asn Val Ile Cys Lys Arg Lys Gly Ile Arg Lys Lys Lys 
            420                 425                 430         


Ile Ser Ala Lys Thr Thr Gln Leu Asp Leu Leu Asn Glu Ser Ser Gln 
        435                 440                 445             


Thr Ile Phe Ser Leu Asp Met Glu Cys Lys Gln Leu Leu Phe Asp Glu 
    450                 455                 460                 


Leu Asn Leu Lys Gly Asp Leu Lys Ser Asp Lys Val Leu Lys Leu Leu 
465                 470                 475                 480 


Gly Tyr Ser Pro Gln Glu Trp Glu Ile Asn Tyr Asn Gln Leu Glu Gly 
                485                 490                 495     


Asn Arg Thr Gln Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Val Glu 
            500                 505                 510         


Met Glu Ala His Asp Val Lys Asp Ile Leu Gln Ile Lys Ser Ala Lys 
        515                 520                 525             


Asp Asp Trp Ser Leu Asp Glu Ser Pro Leu Ser Ala Ser Glu Ile Arg 
    530                 535                 540                 


Glu Lys Val Lys Ala Ile Phe Gln Thr Leu Gly Ile Cys Thr Lys Ile 
545                 550                 555                 560 


Leu Tyr Phe Asp Pro Leu Leu Pro Val Lys Glu Phe Glu Glu Gln Asp 
                565                 570                 575     


Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asp Ser 
            580                 585                 590         


Thr Ser Gly Asn Glu Thr Leu Tyr Arg Ile Leu Glu Lys Lys Tyr Ala 
        595                 600                 605             


Phe Lys Arg Glu His Ala Arg Ile Leu Ala Asn Val Ala Leu Gln Asp 
    610                 615                 620                 


Asp Tyr Gly Ser Leu Ser Thr Lys Ala Ile Arg Lys Ile Tyr Pro Asn 
625                 630                 635                 640 


Ile Lys Glu Asn Gln Tyr Ser Thr Ala Cys Glu Lys Ala Gly Tyr Lys 
                645                 650                 655     


His Ser Lys Leu Ser Leu Thr Thr Glu Glu Leu Glu Ala Arg Glu Leu 
            660                 665                 670         


Lys Asn Ile Ile Pro Leu Leu Lys Lys Asn Ala Leu Arg Asn Pro Val 
        675                 680                 685             


Val Glu Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Ala Leu Ile 
    690                 695                 700                 


Glu Lys Asn Ser Glu Arg Asp Ala Glu Gly Lys Ile Thr Lys Tyr Phe 
705                 710                 715                 720 


His Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn 
                725                 730                 735     


Ala Gln Lys Arg Tyr Glu Met Thr Gln Asn Ile Asn Lys Ala Lys Leu 
            740                 745                 750         


Glu His Gln Lys Ile Ser Glu Ile Leu Gln Lys Glu Phe Gly Ile Lys 
        755                 760                 765             


Asn Pro Thr Lys Ser Asp Ile Ile Arg Tyr Arg Leu Tyr Gln Glu Leu 
    770                 775                 780                 


Glu His Asn Gly Tyr Lys Glu Leu Tyr Thr Asn Ala Pro Ile Ala Arg 
785                 790                 795                 800 


Asp Met Leu Phe Ser Lys Asn Ile Glu Ile Glu His Ile Val Pro Lys 
                805                 810                 815     


Ala Arg Val Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Thr Phe His 
            820                 825                 830         


Arg Ile Asn Ser Asp Lys Gly Glu Tyr Thr Ala Phe Asp Tyr Ile Thr 
        835                 840                 845             


Ser Leu Asn Ser Glu Glu Glu Leu Asn Gln Tyr Leu Thr Arg Val Glu 
    850                 855                 860                 


Asn Ala Tyr Lys Thr Lys Ser Ile Ser Pro Thr Lys Tyr Lys Asn Leu 
865                 870                 875                 880 


Leu Lys Lys Ala Ser Glu Ile Gly Asp Asp Phe Ile Asn Arg Asp Leu 
                885                 890                 895     


Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Phe Gln 
            900                 905                 910         


Val Thr Lys Asn Val Leu Ser Thr Ser Gly Ser Ile Thr Asp Arg Leu 
        915                 920                 925             


Arg Glu Asp Trp Gly Leu Val Asp Val Met Lys Glu Leu Asn Met Pro 
    930                 935                 940                 


Lys Tyr Gln Ser Leu Gly Leu Thr Glu Val Glu Glu Arg Lys Asp Gly 
945                 950                 955                 960 


Asn Lys Val Thr Val Ile Lys Asn Trp Thr Lys Arg Asn Asp His Arg 
                965                 970                 975     


His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Pro Ser Tyr 
            980                 985                 990         


Ile Gln Tyr Leu Asn His Leu Asn  Ala Arg Lys Asp Glu  Asn Asn Lys 
        995                 1000                 1005             


Asn Tyr  Ser Val Ile Leu Ala  Ile Glu Glu Lys Glu  Thr Ile Lys 
    1010                 1015                 1020             


Val Pro  Thr Asn Asn Gly Lys  Asn Lys Arg Val Phe  Ile Glu Pro 
    1025                 1030                 1035             


Ile Pro  Asn Phe Arg Gln Val  Ala Lys Lys His Leu  Glu Glu Ile 
    1040                 1045                 1050             


Phe Ile  Ser His Lys Ala Lys  Asn Lys Val Val Thr  Lys Asn Thr 
    1055                 1060                 1065             


Asn Lys  Pro Ala Gly Thr Asp  Lys Gln Gln Ile Thr  Leu Thr Pro 
    1070                 1075                 1080             


Arg Gly  Gln Leu His Lys Glu  Thr Ile Tyr Gly Lys  Tyr Gln Tyr 
    1085                 1090                 1095             


Tyr Ile  Asn Lys Glu Glu Lys  Ile Gly Val Lys Phe  Asp Glu Arg 
    1100                 1105                 1110             


Thr Ile  Ala Lys Val Ser Asn  Pro Val Tyr Arg Glu  Ala Leu Leu 
    1115                 1120                 1125             


Lys Arg  Leu Gln Ala Asn Asp  Asn Asp Pro Lys Lys  Ala Phe Ala 
    1130                 1135                 1140             


Gly Lys  Asn Ala Leu Ser Lys  Asn Pro Ile Tyr Leu  Asp Glu Ser 
    1145                 1150                 1155             


Lys Thr  Lys Thr Leu Pro Glu  Lys Val Asn Leu Thr  Tyr Leu Glu 
    1160                 1165                 1170             


Glu Asp  Phe Ser Ile Arg Lys  Asp Ile Ser Pro Asp  Asn Phe Lys 
    1175                 1180                 1185             


Asp Leu  Lys Ser Ile Glu Lys  Val Ile Asp Gln Gly  Val Lys Arg 
    1190                 1195                 1200             


Ile Leu  Ile Lys Arg Leu Gln  Ala Tyr Asp Asn Asp  Pro Lys Lys 
    1205                 1210                 1215             


Ala Phe  Val Asp Leu Glu Lys  Asn Pro Ile Trp Leu  Asn Lys Glu 
    1220                 1225                 1230             


Lys Gly  Ile Ala Ile Lys Arg  Val Thr Ile Ser Gly  Val Asn Asn 
    1235                 1240                 1245             


Ala Gln  Pro Leu His Ile Gly  Lys Asp His Leu Gly  Lys Thr Thr 
    1250                 1255                 1260             


Leu Asn  Lys Glu Gly Lys Glu  Ile Pro Val Asp Tyr  Val Ser Thr 
    1265                 1270                 1275             


Gly Asn  Asn His His Val Ala  Ile Tyr Arg Asp Lys  Glu Gly Asn 
    1280                 1285                 1290             


Leu Gln  Glu Gln Ile Val Ser  Phe Phe Asp Ala Val  Val Arg Ala 
    1295                 1300                 1305             


Gln Gln  Gly Ile Pro Ile Ile  Asp Lys Thr Tyr Lys  Gln Ala Glu 
    1310                 1315                 1320             


Gly Trp  Gln Phe Leu Phe Thr  Met Lys Gln Asn Glu  Met Phe Val 
    1325                 1330                 1335             


Phe Pro  Asn Ala Thr Thr Gly  Phe Asn Pro Ala Glu  Ile Asp Leu 
    1340                 1345                 1350             


Leu Asp  Pro Lys Asn Lys Lys  Leu Ile Ser Pro Asn  Leu Phe Arg 
    1355                 1360                 1365             


Val Gln  Lys Ile Ala Thr Lys  Asp Tyr Phe Phe Arg  His His Leu 
    1370                 1375                 1380             


Glu Thr  Asn Val Glu Thr Asp  Asn Ile Leu Lys Asn  Val Thr Trp 
    1385                 1390                 1395             


Lys Arg  Glu Gly Leu Ser Gly  Leu Lys Asp Ile Val  Lys Val Arg 
    1400                 1405                 1410             


Ile Asn  His Leu Gly Asp Ile  Val Ser Ile Gly Glu  Tyr 
    1415                 1420                 1425     


<210>  1226
<211>  1426
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1226

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Phe 
1               5                   10                  15      


Thr His Ile Val Glu Asp Glu Asn Lys Glu Lys Ser Glu Ile Lys Glu 
            20                  25                  30          


Leu Gly Val Arg Ile Val Ser Leu Thr Thr Asp Glu Gln Ser Asp Phe 
        35                  40                  45              


Glu Lys Gly Lys Ser Ile Ser Thr Asn Ala Asn Arg Thr Leu Lys His 
    50                  55                  60                  


Gly Ala Arg Leu Asn Leu Asp Arg Tyr Gln Gln Arg Arg Lys Phe Leu 
65                  70                  75                  80  


Ile Asp Leu Leu Gln Lys Ala Asn Leu Ile Thr Ser Ser Ser Ile Leu 
                85                  90                  95      


Ala Glu Asn Gly Lys Asn Thr Thr His Ser Thr Trp Gln Leu Arg Ala 
            100                 105                 110         


Lys Ala Val Thr Glu Arg Ile Glu Lys Glu Glu Phe Ala Arg Val Leu 
        115                 120                 125             


Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala Lys 
    130                 135                 140                 


Thr Glu Asp Glu Gly Gln Ala Ile Asp Gly Met Ala Ile Ala Lys Arg 
145                 150                 155                 160 


Leu Tyr Asp Glu Asn Leu Thr Pro Gly Gln Leu Ser Leu Gln Leu Leu 
                165                 170                 175     


Gln Gln Asn Lys Lys Leu Leu Pro Asp Phe Tyr Arg Ser Asp Leu Gln 
            180                 185                 190         


Lys Glu Phe Asp Leu Val Trp Asn Phe Gln Lys Gln Phe Tyr Pro Asp 
        195                 200                 205             


Ile Leu Thr Asp Ser Phe Tyr Lys Glu Leu Gln Gly Lys Gly Lys Asp 
    210                 215                 220                 


Ala Thr Ser Lys Ala Phe Ser Lys Arg Tyr His Phe Asp Thr Ala Glu 
225                 230                 235                 240 


Asn Lys Gly Ser Arg Glu Ser Val Arg Leu Gln Ala Tyr Gln Trp Arg 
                245                 250                 255     


Ala Glu Ala Ile Ser Lys Gln Leu Ser Lys Glu Glu Val Ala Tyr Val 
            260                 265                 270         


Leu Thr Glu Ile Asn Asn Asn Leu Asn Asn Ala Ser Gly Tyr Leu Gly 
        275                 280                 285             


Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Arg Gln Thr Val 
    290                 295                 300                 


Gly Gln Tyr Leu Tyr Ala Gln Leu Gln Glu Asn Lys His Asn Ser Leu 
305                 310                 315                 320 


Lys Asn Lys Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Arg 
                325                 330                 335     


Ile Trp Glu Thr Gln Ala Ser Phe His Lys Glu Leu Thr Glu Glu Leu 
            340                 345                 350         


Lys Lys Gln Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Leu Lys 
        355                 360                 365             


Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Lys Glu Ile 
    370                 375                 380                 


Glu Ile Glu Lys Asp Gly Lys Thr Ile Thr Lys Ser Ile Gly Ala Arg 
385                 390                 395                 400 


Val Val Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln 
                405                 410                 415     


Ile Leu Asn Asn Val Thr Cys Lys Arg Lys Gly Ile Arg Lys Lys Lys 
            420                 425                 430         


Ser Ser Ala Lys Ser Thr Glu Leu Asp Leu Phe Asn Glu Pro Ser Gln 
        435                 440                 445             


Thr Ile Phe Ser Leu Asp Met Glu Cys Lys Gln Leu Leu Phe Glu Glu 
    450                 455                 460                 


Leu Asn Leu Lys Gly Asp Leu Lys Ser Asp Lys Val Leu Lys Leu Leu 
465                 470                 475                 480 


Gly Tyr Ser Pro Gln Glu Trp Glu Ile Asn Tyr Asn Gln Leu Glu Gly 
                485                 490                 495     


Asn Arg Thr Gln Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Val Glu 
            500                 505                 510         


Met Glu Ala His Asp Val Lys Asp Ile Leu Gln Ile Lys Ser Thr Lys 
        515                 520                 525             


Asp Asp Trp Ala Leu Glu Glu Ser Pro Leu Ser Ala Ser Glu Ile Arg 
    530                 535                 540                 


Glu Arg Val Lys Ala Ile Phe Gln Thr Leu Gly Ile Cys Thr Lys Ile 
545                 550                 555                 560 


Leu Tyr Phe Asp Pro Leu Leu Pro Val Lys Glu Phe Glu Glu Gln Asp 
                565                 570                 575     


Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asp Ser 
            580                 585                 590         


Thr Ser Gly Asn Glu Thr Leu Tyr Arg Ile Leu Glu Lys Lys Tyr Ala 
        595                 600                 605             


Phe Lys Arg Glu His Ala Thr Ile Leu Ala Asn Val Thr Leu Gln Asp 
    610                 615                 620                 


Asp Tyr Gly Ser Leu Ser Thr Lys Ala Ile Arg Lys Ile Tyr Pro Tyr 
625                 630                 635                 640 


Ile Lys Glu Asn Gln Tyr Ser Thr Ala Cys Glu Lys Ala Gly Tyr Lys 
                645                 650                 655     


His Ser Lys Leu Ser Leu Thr Thr Glu Glu Leu Glu Ala Arg Glu Leu 
            660                 665                 670         


Lys Asn Ile Ile Pro Leu Leu Lys Lys Asn Ala Leu Arg Asn Pro Val 
        675                 680                 685             


Val Glu Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Ala Leu Ile 
    690                 695                 700                 


Glu Lys Asn Ser Glu Arg Asp Ala Glu Gly Lys Ile Thr Lys Tyr Phe 
705                 710                 715                 720 


His Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn 
                725                 730                 735     


Ala Gln Glu Arg Tyr Glu Met Thr Gln Asn Ile Asn Lys Ala Lys Leu 
            740                 745                 750         


Glu His Gln Lys Ile Ser Gly Ile Leu Gln Lys Glu Phe Gly Ile Lys 
        755                 760                 765             


Asn Pro Thr Lys Ser Asp Ile Thr Arg Tyr Arg Leu Tyr Gln Glu Leu 
    770                 775                 780                 


Ala His Asn Gly Tyr Asn Asp Leu Tyr Thr Asn Thr Tyr Ile Pro Lys 
785                 790                 795                 800 


Glu Leu Leu Phe Ser Lys Asn Ile Asp Ile Glu His Ile Val Ser Lys 
                805                 810                 815     


Ala Arg Val Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Ala Phe Arg 
            820                 825                 830         


Glu Phe Asn Ile Lys Lys Gly Glu Ser Thr Ala Phe Asp Tyr Ile Thr 
        835                 840                 845             


Ser Leu Asn Asn Glu Glu Glu Leu Asn Gln Tyr Leu Asn Arg Val Glu 
    850                 855                 860                 


Asn Ala Tyr Lys Thr Lys Asn Ile Ser Pro Ala Lys Tyr Lys Asn Leu 
865                 870                 875                 880 


Leu Lys Lys Ala Ser Glu Ile Gly Asp Gly Phe Ile Asn Arg Asp Leu 
                885                 890                 895     


Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Phe Gln 
            900                 905                 910         


Val Thr Lys Asn Val Leu Ser Thr Ser Gly Ser Ile Thr Asp Arg Leu 
        915                 920                 925             


Arg Glu Asp Trp Gly Leu Val Asp Val Met Lys Glu Leu Asn Met Pro 
    930                 935                 940                 


Lys Tyr Gln Ser Leu Gly Leu Thr Glu Val Glu Glu Arg Lys Glu Gly 
945                 950                 955                 960 


Asn Lys Val Thr Val Ile Lys Asn Trp Thr Lys Arg Asn Asp His Arg 
                965                 970                 975     


His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Pro Ser Tyr 
            980                 985                 990         


Ile Gln Tyr Leu Asn His Leu Asn  Ala Arg Lys Asp Lys  Asn Asn Lys 
        995                 1000                 1005             


Asn Tyr  Ser Val Ile Leu Ala  Ile Glu Glu Lys Glu  Thr Ile Lys 
    1010                 1015                 1020             


Val Pro  Thr Asn Asn Gly Lys  Asn Lys Arg Val Phe  Ile Glu Pro 
    1025                 1030                 1035             


Ile Pro  Asn Phe Arg Gln Val  Ala Lys Lys His Leu  Glu Glu Ile 
    1040                 1045                 1050             


Phe Ile  Ser His Lys Ala Lys  Asn Lys Val Val Thr  Lys Asn Thr 
    1055                 1060                 1065             


Asn Lys  Pro Ala Gly Thr Asp  Lys Gln Gln Ile Thr  Leu Thr Pro 
    1070                 1075                 1080             


Arg Gly  Gln Leu His Lys Glu  Thr Val Tyr Gly Lys  Tyr Gln Tyr 
    1085                 1090                 1095             


Tyr Val  Asn Lys Glu Glu Lys  Ile Gly Val Lys Phe  Asp Lys Glu 
    1100                 1105                 1110             


Thr Ile  Ala Lys Val Ser Asn  Pro Val Tyr Arg Glu  Ala Leu Leu 
    1115                 1120                 1125             


Lys Arg  Leu Gln Ala Asn Asp  Asn Asp Pro Lys Lys  Ala Phe Ala 
    1130                 1135                 1140             


Gly Lys  Asn Ala Leu Ser Lys  Asn Pro Ile Tyr Leu  Asp Glu Ala 
    1145                 1150                 1155             


Lys Thr  Lys Thr Leu Thr Glu  Lys Val Asn Leu Thr  Tyr Leu Glu 
    1160                 1165                 1170             


Glu Asp  Phe Ser Ile Arg Lys  Asp Ile Ser Pro Asp  Asn Phe Lys 
    1175                 1180                 1185             


Asp Leu  Lys Ser Ile Glu Lys  Val Ile Asp Gln Gly  Val Lys Arg 
    1190                 1195                 1200             


Ile Leu  Ile Lys Arg Leu Gln  Asp Tyr Asp Asn Asp  Pro Lys Lys 
    1205                 1210                 1215             


Ala Phe  Val Asp Leu Glu Lys  Asn Pro Ile Trp Leu  Asn Lys Glu 
    1220                 1225                 1230             


Lys Gly  Ile Ala Ile Lys Arg  Val Thr Ile Ser Gly  Val Asn Asn 
    1235                 1240                 1245             


Ala Gln  Pro Leu His Ile Gly  Lys Asp His Leu Gly  Lys Thr Thr 
    1250                 1255                 1260             


Leu Asn  Lys Glu Gly Lys Glu  Ile Pro Val Asp Tyr  Val Ser Thr 
    1265                 1270                 1275             


Gly Asn  Asn His His Val Ala  Ile Tyr Arg Asp Lys  Glu Gly Asn 
    1280                 1285                 1290             


Leu Gln  Glu Gln Ile Val Ser  Phe Phe Glu Ala Val  Val Gly Ala 
    1295                 1300                 1305             


Gln Gln  Gly Leu Pro Ile Val  Asp Lys Thr Tyr Lys  Gln Ala Glu 
    1310                 1315                 1320             


Gly Trp  Gln Phe Leu Phe Thr  Met Lys Gln Asn Glu  Met Phe Val 
    1325                 1330                 1335             


Phe Pro  Asn Glu Ala Thr Gly  Phe Asn Pro Ala Glu  Ile Asp Leu 
    1340                 1345                 1350             


Leu Asp  Pro Lys Asn Lys Lys  Gln Ile Ser Pro Asn  Leu Phe Arg 
    1355                 1360                 1365             


Val Gln  Lys Ile Ala Thr Lys  Asp Tyr Phe Phe Arg  His His Leu 
    1370                 1375                 1380             


Glu Thr  Asn Val Asp Thr Asp  Asn Val Leu Lys Asn  Thr Thr Trp 
    1385                 1390                 1395             


Lys Arg  Glu Gly Leu Ser Gly  Leu Lys Asp Ile Val  Lys Val Arg 
    1400                 1405                 1410             


Ile Asn  His Leu Gly Asp Ile  Val Ser Ile Gly Glu  Tyr 
    1415                 1420                 1425     


<210>  1227
<211>  1427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1227

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Thr Ser Glu Glu Lys Ser Ser Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Gln Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Ala Gly 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Asn 
65                  70                  75                  80  


Leu Ile Glu Val Leu Lys Glu Tyr Asn Phe Ile Thr Asp Glu Met Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Val Glu Glu Ile Ser Leu Glu Gln Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Ala Glu Asn Gly Thr Leu Ile Asp Gly Met Glu Val Ala Lys 
145                 150                 155                 160 


Lys Met Tyr Asp Glu Gly Ile Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Thr Ala Gly Lys Arg Tyr Phe Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asp Arg Ile Trp Asn Phe Gln Lys Gln Phe Tyr Pro 
        195                 200                 205             


Asp Ser Leu Ile Asp Lys Val Lys Asp Glu Val Arg Gly Lys Asn Lys 
    210                 215                 220                 


Ser Gln Thr Trp Ala Ile Leu Ala Lys Tyr Phe Val Trp Lys Glu Val 
225                 230                 235                 240 


Glu Asn Ser Trp Asn Glu Glu Glu Ala Gln Thr Arg Arg Val Glu Lys 
                245                 250                 255     


Glu Tyr Arg Leu Val Gly Ile Lys Arg Gly Val Lys Arg Glu Glu Leu 
            260                 265                 270         


Lys Leu Glu Asn Phe Gln Trp Arg Val Lys Ala Leu Ser Glu Arg Met 
        275                 280                 285             


Asn Pro Glu Glu Leu Ala Ile Val Leu Gln Glu Ile Asn Glu Gln Ile 
    290                 295                 300                 


Ser Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Arg Gln Thr Val Gly Gln Tyr Gln Met Ala Val Leu 
                325                 330                 335     


Asp Asn Asn Pro Asn Ile Gly Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Thr Ile Trp Glu Lys Gln Ala Glu Phe 
        355                 360                 365             


His Lys Glu Leu Thr Glu Asp Leu Lys Lys Glu Ile Arg Asp Ile Val 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Asn Ile 
385                 390                 395                 400 


Cys Glu Phe Glu Arg Arg Gln Ile Glu Val Glu Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Ile Gly Ser Arg Val Ile Pro Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Thr Leu Asn Asp Ile Glu Val Ser 
        435                 440                 445             


Val Leu Gly Val Lys Asn Lys Arg Lys Lys Gln Asp Asp Asn Ser Thr 
    450                 455                 460                 


Thr Leu Leu Asp Ser Ala Glu Asn Ile Asp Ser Leu Lys Leu Asn Val 
465                 470                 475                 480 


Ser Arg Pro Leu Asp Ala Asp Glu Lys Ser Leu Leu Ala Lys Glu Leu 
                485                 490                 495     


Phe Ile Arg Asp Lys Leu Thr Lys Ser Asp Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asn Asn Pro Gln Asn Leu Asn Leu Asn Phe Lys Asn Ile Asp Gly Asn 
        515                 520                 525             


Arg Thr Gly Ser Ala Leu Tyr Gln Ala Phe Ser Lys Ile Leu Glu Ile 
    530                 535                 540                 


Ser Gly His Glu Ser Ile Asn Phe Lys Lys Ser Ala Asp Glu Ile Val 
545                 550                 555                 560 


Glu Gln Val Lys Thr Ile Phe Ser Ala Leu Gly Trp Asn Thr Glu Val 
                565                 570                 575     


Leu Cys Phe Asp Ser Glu Lys Glu Leu Asp Lys Gln Pro Tyr Phe Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Ser Thr Pro Thr Gly 
        595                 600                 605             


Asp Gly Asn Leu Ile Gln Lys Ile Ala Asp Leu Cys Gly Phe Glu Lys 
    610                 615                 620                 


Asp Tyr Ala Ser Ile Leu Ala Asn Ile Thr Phe Gln Glu Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Thr Lys Ala Ile Arg Lys Ile Leu Pro His Leu Lys Asp 
                645                 650                 655     


Gly Asn Gln Tyr Asp Val Ala Cys Glu Tyr Ala Gly Tyr Lys His Ser 
            660                 665                 670         


Lys Ser Ser Leu Lys Lys Glu Glu Ile Lys Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Lys Leu Glu Leu Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Ala Ile Ile Thr Thr 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Ser Ile Val Asp Thr 
            740                 745                 750         


Thr Lys Thr His Glu Gly Ile Arg Ala Ile Leu Gln Asn Asp Phe Gly 
        755                 760                 765             


Met Ser His Val Ser Arg Asn Ala Ile Ile Arg Tyr Lys Leu Tyr Glu 
    770                 775                 780                 


Glu Leu Lys Asp Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Pro Lys Glu Lys Leu Phe Ser Lys Asp Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Val Lys Ser Ile Asn Ile Glu Lys Gly Ser Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Glu Glu Lys Tyr Gly Glu Gln Gly Leu Gln Glu Tyr Leu Asn Arg 
    850                 855                 860                 


Cys Glu Val Leu Phe Arg Asp Lys Lys Thr Lys Leu Arg Lys Leu Lys 
865                 870                 875                 880 


Met Gln Glu Ser Glu Ile Pro Glu Gly Phe Ile Asp Arg Asp Leu Arg 
                885                 890                 895     


Asn Thr Gln Tyr Ile Ala Lys Lys Ala Leu Ser Met Leu Asn Glu Ile 
            900                 905                 910         


Cys Arg Arg Val Val Ala Thr Thr Gly Ala Ile Thr Asp Glu Leu Arg 
        915                 920                 925             


Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn Trp Glu Lys 
    930                 935                 940                 


Tyr Lys Val Leu Gly Leu Val Glu Tyr Phe Glu Asp Asn Asp Gly Arg 
945                 950                 955                 960 


Leu Ile Gly Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His 
                965                 970                 975     


His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Asp Val Phe Ile 
            980                 985                 990         


Gln Tyr Phe Asn Asn Lys Asn Ala  Ser Leu Lys Pro Asp  Thr Asn Glu 
        995                 1000                 1005             


Ala Ala  Ile Arg Asn Lys Tyr  Phe Gln Asn Gly Lys  Ala Ile Pro 
    1010                 1015                 1020             


Pro Met  Ser Leu Asp Glu Phe  Arg Ala Glu Ala Lys  Lys His Leu 
    1025                 1030                 1035             


Asn Asp  Thr Leu Ile Ser Ile  Lys Thr Lys Asn Lys  Val Val Thr 
    1040                 1045                 1050             


Ile Asn  Leu Asn Arg Thr Lys  Lys Lys Asn Gly Ile  Asn Lys Lys 
    1055                 1060                 1065             


Gln Gln  Gln Thr Pro Arg Gly  Gln Leu His Leu Glu  Thr Val Tyr 
    1070                 1075                 1080             


Gly Ser  His Lys Gln Tyr Val  Val Lys Glu Glu Lys  Val Asn Ala 
    1085                 1090                 1095             


Ser Phe  Asp Ala Ala Lys Ile  Ala Thr Val Asn Lys  Pro Ala Tyr 
    1100                 1105                 1110             


Arg Asp  Ala Leu Leu Arg Arg  Leu Gln Glu Asn Gly  Asn Asp Ser 
    1115                 1120                 1125             


Lys Lys  Ala Phe Thr Gly Lys  Asn Ser Leu Asp Lys  Gln Pro Val 
    1130                 1135                 1140             


Trp Leu  Asp Ala Lys Gln Asn  Glu Cys Val Pro Glu  Lys Val Lys 
    1145                 1150                 1155             


Thr Val  Met Leu Glu Thr Val  Tyr Thr Val Arg Lys  Pro Ile Asp 
    1160                 1165                 1170             


Glu Lys  Leu Asn Val Asp Lys  Val Val Asp Ala Arg  Ile Arg Lys 
    1175                 1180                 1185             


Leu Leu  Gln Asp Arg Ile Lys  Glu Tyr Gly Asn Ala  Lys Ser Ala 
    1190                 1195                 1200             


Phe Ala  Asn Leu Asp Glu Asn  Pro Ile Trp Leu Asn  Lys Glu Lys 
    1205                 1210                 1215             


Gly Ile  Thr Ile Lys Arg Val  Ser Val Ser Gly Ile  Ser Lys Ala 
    1220                 1225                 1230             


Gln Ser  Leu His Glu Lys Lys  Asp Lys Asp Gly Asn  Phe Ile Leu 
    1235                 1240                 1245             


Asp Lys  Asp Ser Arg Arg Ile  Pro Val Asp Phe Val  Asn Thr Gly 
    1250                 1255                 1260             


Asn Asn  His His Val Ala Ile  Tyr Arg Lys Pro Val  Leu Asp Lys 
    1265                 1270                 1275             


Lys Gly  Gln Ala Val Phe Asp  Glu Asn Gly Glu Leu  Lys Phe Glu 
    1280                 1285                 1290             


Leu Glu  Glu Val Val Val Ser  Phe Phe Asp Ala Val  Thr Arg Val 
    1295                 1300                 1305             


Asn Leu  Ser Gln Pro Ile Ile  Asp Lys Asp Tyr Lys  Arg Ser Glu 
    1310                 1315                 1320             


Gly Trp  Lys Phe Leu Phe Ser  Met Lys Gln Asn Glu  Tyr Phe Val 
    1325                 1330                 1335             


Phe Pro  Asn Glu Lys Thr Gly  Phe Asp Pro Lys Glu  Ile Asp Leu 
    1340                 1345                 1350             


Leu Asn  Pro Glu Asn Tyr Ala  Leu Ile Ser Pro Asn  Leu Phe Arg 
    1355                 1360                 1365             


Val Gln  Lys Ile Ala Ser Lys  Asp Tyr Val Phe Arg  His His Leu 
    1370                 1375                 1380             


Glu Thr  Val Val Ala Asp Arg  Lys Glu Leu Gln Glu  Ile Thr Trp 
    1385                 1390                 1395             


Lys Arg  Ile Ser Ser Leu Ser  Ile Leu Asn Arg Ile  Ile Lys Val 
    1400                 1405                 1410             


Arg Val  Asn His Ile Gly Gln  Ile Val Met Ile Gly  Glu Tyr 
    1415                 1420                 1425         


<210>  1228
<211>  1427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1228

Met Ala Lys Pro Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn 
1               5                   10                  15      


Ser Val Gly Trp Val Val Thr Asp Asp Gln Asn Asn Ile Leu Arg Ile 
            20                  25                  30          


Lys Gly Lys Lys Ala Ile Gly Ala Arg Leu Phe Ala Glu Gly Lys Val 
        35                  40                  45              


Ala Ala Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg 
    50                  55                  60                  


Arg Arg Trp Arg Ile Lys Leu Leu Glu Glu Leu Phe Asp Lys Glu Ile 
65                  70                  75                  80  


Ala Lys Val Asp Pro Ser Phe Phe Ala Arg Leu His Glu Ser Trp Ile 
                85                  90                  95      


Ser Pro Lys Asp Lys Arg Lys Arg Tyr Ser Ala Ile Val Phe Pro Ser 
            100                 105                 110         


Pro Glu Glu Asp Lys Lys Phe His Glu Ser Tyr Pro Thr Ile Tyr His 
        115                 120                 125             


Leu Arg Asp Lys Leu Met Lys Asp Asp Gln Lys His Asp Ile Arg Glu 
    130                 135                 140                 


Ile Tyr Ile Ala Val His Gln Met Ile Lys Ala Arg Gly Asn Phe Leu 
145                 150                 155                 160 


His Asp Glu Ser Val Glu Thr Tyr Arg Ser Gly Met Ser Ser Leu Gly 
                165                 170                 175     


Gly Arg Ser Glu Arg Thr Ile Leu Ser Val Gln Thr Leu Glu Glu Leu 
            180                 185                 190         


Asn Asp Leu Phe Ala Glu Asn Glu Gly Thr Glu Glu Ala Lys Leu Asn 
        195                 200                 205             


Val Ala Ser Ala Glu Gln Ile Asn Asp Ile Leu Thr Gly Gly His Leu 
    210                 215                 220                 


Asn Ala Asp Ser Gln Lys Glu Ile Gly Asn Leu Leu Leu Pro Ser Ser 
225                 230                 235                 240 


Phe Pro Ser Phe Asp Asp Lys Ala Lys Glu Lys Gln Val Lys Lys Leu 
                245                 250                 255     


Ile Lys Asn Val Ala Thr Asn Ile Ser Lys Ala Trp Leu Gly Tyr Lys 
            260                 265                 270         


Ala Asp Phe Ser Thr Ile Leu Asn Leu Ala Asn Val Asp Lys Asp Gln 
        275                 280                 285             


Lys Lys Ile Phe Ala Phe Ala Leu Gln Gly Gly Asp Glu Glu Asp Lys 
    290                 295                 300                 


Val Gln Glu Leu Glu Ser Leu Leu Glu Gln Ser Gln Thr Asp Ile Val 
305                 310                 315                 320 


Asp Arg Leu Ile Glu Ile Arg His Ala Ile Val Leu Ser Glu Ile Val 
                325                 330                 335     


Pro Val Gly Met Thr Leu Ser Glu Ala Met Ile Asp Lys Tyr Asp Gln 
            340                 345                 350         


His Lys Glu Asp Leu Ile Thr Leu Lys Ala Val Ile Arg Asn Thr Lys 
        355                 360                 365             


Asp Lys Lys Lys Ala Ala Lys Leu Gln Ala Ile Tyr Asp Leu Tyr Val 
    370                 375                 380                 


Asn Asn Arg His Ala Asp Leu Ala Lys Ala Met Lys Leu Thr Gly Ile 
385                 390                 395                 400 


Lys Lys Arg Ser Glu Leu Leu Asp Pro Glu Glu Leu Lys Lys Ala Ile 
                405                 410                 415     


Ser Ser Leu Leu Asp Asp Ser Pro Glu Ala Val Glu Ile Lys Gln Met 
            420                 425                 430         


Leu Glu Glu Lys Thr Phe Leu Pro Leu Gln Arg Ser Asn Asn Asn Gly 
        435                 440                 445             


Val Ile Pro Asn Gln Leu His Gln Val Glu Leu Asp Glu Ile Ile Lys 
    450                 455                 460                 


Lys Gln Ser Lys Tyr Tyr Pro Phe Leu Ala Glu Lys Asn Pro Asp Glu 
465                 470                 475                 480 


Ser Glu Glu Ala Gln Lys Lys Ala Pro Thr Lys Leu Asp Ala Leu Leu 
                485                 490                 495     


Thr Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Lys Glu Glu 
            500                 505                 510         


Gln Glu Ala Gln Asp Gly His Ser Phe Ala Trp Met Val Arg Arg Asp 
        515                 520                 525             


Pro Gln Asp His Glu Ala Ile Thr Pro Trp Asn Phe Glu Lys Lys Val 
    530                 535                 540                 


Asp Lys Met Ala Ser Ala Thr Gln Phe Ile Lys Arg Met Thr Thr Lys 
545                 550                 555                 560 


Asp Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Ala Ser Ser Leu Lys 
                565                 570                 575     


Tyr Gln Leu Phe Thr Val Leu Asn Glu Leu Asn Asn Leu Arg Val Asn 
            580                 585                 590         


Gly Lys Lys Leu Thr Ser Asp Glu Lys Glu Gln Val Ile Glu Gly Leu 
        595                 600                 605             


Phe Lys Lys Gln Lys Thr Val Lys Ala Asp Lys Phe Val Lys Tyr Trp 
    610                 615                 620                 


Gln Ala Lys His Ile Gly Ala Asp Ile Lys Val Lys Gly Leu Ser Asp 
625                 630                 635                 640 


Pro Ser Lys Phe Asn Ser Thr Met Ser Thr Tyr Ile Asp Phe Lys Lys 
                645                 650                 655     


Ile Phe Gly Asp Gln Leu Asn Asp Val Asn Arg Gln Asn Asp Phe Glu 
            660                 665                 670         


Lys Ile Ile Glu Tyr Ser Thr Ile Phe Glu Asp Arg Lys Ile Leu Glu 
        675                 680                 685             


Asp Lys Leu Arg Ala Asp Phe Ala Trp Leu Ser Glu Glu Gln Ile Lys 
    690                 695                 700                 


Gln Leu Cys Arg Leu Arg Leu Gln Gly Trp Gly Arg Leu Ser Asp Lys 
705                 710                 715                 720 


Leu Leu Thr Arg Leu Thr Asp Ala Asp Gly Gln Asn Val Leu Glu Arg 
                725                 730                 735     


Leu Trp Asn Ser Asn Asp Asn Phe Val Gln Ile Val Ser Asp Pro Asn 
            740                 745                 750         


Ile Lys Ala Lys Ile Glu Gln Glu Asn Gln Lys Leu Leu Arg Asp Gly 
        755                 760                 765             


Gln Ser Asp Asp Ala Val Glu Ser Ile Leu Asp Asp Ala Tyr Thr Ser 
    770                 775                 780                 


Pro Gln Asn Lys Lys Ala Ile Arg Gln Val Met Arg Val Val His Asp 
785                 790                 795                 800 


Ile Val Lys Ala Cys Asp Gly Asn Val Pro Ala Lys Phe Ala Ile Glu 
                805                 810                 815     


Phe Ala Arg Ser Gln Glu Asp Asp Pro Lys Arg Thr Thr Ala Arg Val 
            820                 825                 830         


Asn Gln Met Gln Lys Ile Tyr Asp Gln Ile Ser Asp Glu Val Val Ser 
        835                 840                 845             


Gln Gly Val Lys Glu Gln Leu Ser Gly Met Lys Ser Leu Lys Asp Arg 
    850                 855                 860                 


Tyr Tyr Leu Tyr Phe Met Gln Gly Gly Arg Asp Ala Tyr Thr Gly Ala 
865                 870                 875                 880 


Lys Leu Asn Ile Asp Arg Leu Ser Asp Tyr Asp Ile Asp His Ile Met 
                885                 890                 895     


Pro Gln Ser Phe Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val 
            900                 905                 910         


Ala Arg Ala Val Asn Asn Gln Lys Ser Asp Lys Val Pro Ala Leu Leu 
        915                 920                 925             


Phe Gly Asn Lys Val Val Ala Asp Leu Gly Ile Thr Val Arg Glu Met 
    930                 935                 940                 


Trp Asp Lys Trp Gln Lys Leu Gly Met Ile Ser Lys Arg Lys Leu Ser 
945                 950                 955                 960 


Asn Leu Leu Thr Asp Pro Asp Ala Leu Thr Glu Tyr Arg Ala Gln Gly 
                965                 970                 975     


Phe Ile Arg Arg Gln Leu Val Glu Thr Ser Gln Val Ile Lys Leu Thr 
            980                 985                 990         


Ala Thr Ile Leu Gln Ser Glu Phe  Pro Asp Ser Lys Ile  Ile Glu Val 
        995                 1000                 1005             


Pro Ala  Lys Tyr Asn Ser Ile  Val Arg Lys Gln Phe  Asp Leu Tyr 
    1010                 1015                 1020             


Lys Ser  Arg Glu Val Asn Asp  Tyr His His Ala Ile  Asp Ala Tyr 
    1025                 1030                 1035             


Leu Ser  Thr Ile Val Gly Asn  Tyr Leu Tyr Gln Val  Tyr Pro Asn 
    1040                 1045                 1050             


Leu Arg  Arg Met Phe Val Tyr  Gly Glu Phe Lys Lys  Phe Ser Ser 
    1055                 1060                 1065             


Asn Ala  Glu Glu Ser Ala Tyr  Asp Val Val Arg Arg  Val Lys Ser 
    1070                 1075                 1080             


Met Asn  Phe Leu Asp Asp Leu  Leu Arg Gly Thr His  Gly Asp Asn 
    1085                 1090                 1095             


Ile Tyr  Cys Arg Ser Thr Gly  Glu Ile Val Phe Asn  Arg Asn Asp 
    1100                 1105                 1110             


Ile Ile  Ser Lys Leu Lys Gln  Ala Tyr Ser Phe Lys  Gln Met Leu 
    1115                 1120                 1125             


Val Thr  Gln Glu Val Phe Thr  Lys Lys Ser Ala Leu  Phe Asp Gln 
    1130                 1135                 1140             


Thr Val  Tyr Pro Ser Pro Glu  Arg Asp Ser Lys Lys  Arg Ser Gly 
    1145                 1150                 1155             


Leu Ile  Pro Arg Lys Lys Gly  Met Asp Thr Glu Ile  Tyr Gly Gly 
    1160                 1165                 1170             


Tyr Ser  Gly Asn Lys Asp Ala  Tyr Phe Val Leu Ala  Glu Ala Val 
    1175                 1180                 1185             


Lys Glu  Lys Gly His Thr Leu  Gln Ile Val Gly Val  Pro Ile Arg 
    1190                 1195                 1200             


Ala Leu  Asn Thr Leu Lys Asn  Ser Ala Asn Tyr Ser  Glu Lys Leu 
    1205                 1210                 1215             


Leu Glu  Ile Ile Lys Pro Gln  Val Met Phe Asn Lys  Asp Thr Gly 
    1220                 1225                 1230             


Lys Pro  Ile Lys Gly Ile Lys  Asp Val Lys Ile Leu  Met Asp Lys 
    1235                 1240                 1245             


Ile Pro  Cys Arg Gln Pro Val  Leu Glu Gly Glu Ser  Tyr Tyr Met 
    1250                 1255                 1260             


Leu Ala  Ser Ser Lys Tyr Arg  Tyr Ser Leu Lys Gln  Ile Ser Leu 
    1265                 1270                 1275             


Ser Gln  Met Ser Met Lys Tyr  Ile Leu Asp Tyr Ile  Asp Asp Pro 
    1280                 1285                 1290             


Asn Phe  Asn Lys His Glu Met  Ile Asn Ile Asp Gln  Gln Asp Glu 
    1295                 1300                 1305             


Lys Glu  Cys Leu Leu Ser Val  Tyr Asp Glu Ile Leu  Glu Lys Met 
    1310                 1315                 1320             


Asp Lys  Tyr Leu Pro Leu Phe  Asp Ile Arg Ser Phe  Arg Lys Lys 
    1325                 1330                 1335             


Leu His  Asp Gly Arg Asp Ala  Phe Ile Ala Leu Pro  Val Ala Ser 
    1340                 1345                 1350             


Glu Glu  Lys Lys Pro Gly Lys  Val Asp Val Ile Arg  Lys Val Leu 
    1355                 1360                 1365             


Lys Gly  Leu His Ala Asn Ala  Asp Ile Thr Asn Leu  Ala Glu Leu 
    1370                 1375                 1380             


Gly Phe  Gly Thr Ala Ala Leu  Gly Ala Leu Val Ser  Thr Gly Gly 
    1385                 1390                 1395             


Ile Lys  Ile Ser Asp Asp Ala  Val Phe Ile Tyr Gln  Ser Pro Thr 
    1400                 1405                 1410             


Gly Leu  Phe Glu Arg Arg Val  Lys Val Ser Asp Leu  Leu Lys 
    1415                 1420                 1425         


<210>  1229
<211>  1428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1229

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Ser 
1               5                   10                  15      


Ala Tyr Val Leu Glu Ala Glu Lys Ser Ala Glu Gln Thr Lys Ile Val 
            20                  25                  30          


Tyr Leu Gly Thr Arg Ile Ile Leu Ser Ser Ala Asp Ala Glu Asn Phe 
        35                  40                  45              


Ser Lys Gly Leu Ser Cys Ser Pro Thr Thr Asp Arg Thr Phe Lys Arg 
    50                  55                  60                  


Ser Met Arg Arg Arg Leu Gln Arg Tyr Lys Gln Arg Arg Ser Ser Leu 
65                  70                  75                  80  


Ile Gln Gln Phe Lys Lys Ile Gly Ile Ile Thr Asn Thr Glu Gln Ile 
                85                  90                  95      


Ile Arg Glu Pro Ile Ser Glu Thr His Glu Leu Leu Arg Ile Arg Ser 
            100                 105                 110         


Lys Ala Val Ser Glu Arg Val Ser Leu Glu Glu Phe Ala Arg Ile Leu 
        115                 120                 125             


Leu Thr Ile Asn Lys Arg Arg Gly Tyr Lys Ser Asn Arg Lys Thr Ser 
    130                 135                 140                 


Lys Ser Thr Glu Glu Asp Gly Lys Leu Val Asp Ala Met Ala Val Thr 
145                 150                 155                 160 


Lys Glu Leu Ile Lys Leu Lys Lys Thr Pro Gly Gln Tyr Met Phe Glu 
                165                 170                 175     


Arg Phe Lys Arg Gly Lys Tyr Ala Glu Ile Asp Phe Tyr Pro Phe Asp 
            180                 185                 190         


Leu Ile Asn Glu Leu Lys Gln Ile Trp Glu Val Gln Gly Gln Lys Tyr 
        195                 200                 205             


Pro Asp Val Leu Thr Glu Asp Leu Leu Ser Ser Leu Leu Lys Gln Lys 
    210                 215                 220                 


Thr Gly Lys Ala Lys Glu Leu Ile Tyr Ser Lys Ser Asp Leu Asn Pro 
225                 230                 235                 240 


Ala Glu Val Pro Ile Asp Tyr Gln Lys Gly Lys Lys Leu Arg Lys Thr 
                245                 250                 255     


Ile Leu Asn Lys Tyr Phe Ala Tyr Glu Ser Arg Ser Leu Ala Ala Thr 
            260                 265                 270         


Asn Ile Ile Pro Val Glu Gln Val Ile Thr Ala Ile Val Ala Ile Cys 
        275                 280                 285             


Lys Gln Ile Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Gly Asp Arg 
    290                 295                 300                 


Ser Arg Val Leu Tyr Ala Asn Asn Gln Thr Ile Gly Gln Tyr Leu Tyr 
305                 310                 315                 320 


Glu Arg Ile Lys Ala Asn Pro His Asp Arg Ile Arg Gly Glu Val Phe 
                325                 330                 335     


Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Asp Arg Ile Trp Glu Cys Gln 
            340                 345                 350         


Gln Asn Tyr Tyr Pro Ala Ile Leu Thr Asn Asp Arg Lys Gln Phe Ile 
        355                 360                 365             


Arg Asn Arg Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser 
    370                 375                 380                 


Glu Leu Ala Phe Cys Glu Phe Glu Ser Arg Glu Val Glu His Asn Gly 
385                 390                 395                 400 


Lys Lys Tyr Thr Glu Gly Leu His Val Ala Pro Lys Ser Ser Pro Val 
                405                 410                 415     


Phe Gln His Phe Arg Leu Trp Gln Arg Leu Gly Gln Ile Glu Val Ser 
            420                 425                 430         


Pro Leu Arg Lys Trp Asn Lys Gln Ser Asn Glu Leu Phe Glu Gln Gln 
        435                 440                 445             


Asn Asp Thr Asp Gln Glu Arg Ile Leu Asn Thr Glu Ala Lys Gln Lys 
    450                 455                 460                 


Leu Phe Ser Tyr Leu Asn Leu Leu Asp His Leu Thr Asp Asn Glu Ile 
465                 470                 475                 480 


Leu Asn Ile Leu Gly Tyr Ser Pro Asp Lys Trp Lys Ile Asn Phe Arg 
                485                 490                 495     


Glu Ile Ile Gly Asn Arg Thr Asn Asn Ser Met Met Lys Ala Phe Phe 
            500                 505                 510         


Asn Met Lys Glu Met Thr Asp Gly Glu Gln Ile Asn Trp Glu Lys Asn 
        515                 520                 525             


Leu Thr Lys Ile Thr Asn Gly Gln Ser Leu Ile Ser Pro Glu Gln Ile 
    530                 535                 540                 


Val Asp Leu Ile Arg Gln Phe Phe Glu Arg Glu Gly Ile Thr Thr Ser 
545                 550                 555                 560 


Leu Leu Tyr Phe Asp Pro Thr Thr Asp Asn Phe Arg Glu Gln Ser His 
                565                 570                 575     


Tyr Ser Leu Trp His Leu Leu Tyr Ser Tyr Glu Asp Asp Asn Ser Pro 
            580                 585                 590         


Thr Gly Thr Thr Ser Leu Cys Lys Thr Leu Ala Lys Arg Tyr Gly Ile 
        595                 600                 605             


Pro His Glu Leu Cys Pro Leu Leu Ala Ser Leu Thr Phe Glu Pro Gln 
    610                 615                 620                 


Tyr Gly Ser Leu Ser Thr Lys Ala Met Arg Lys Ile Leu Pro Phe Met 
625                 630                 635                 640 


Gln Glu Asn Gly Tyr Asp Lys Ala Cys Glu Leu Ala Gly Tyr Arg His 
                645                 650                 655     


Ser Ala Trp Thr Thr Lys Glu Glu Asn Thr Glu Arg Thr Leu Lys Asn 
            660                 665                 670         


Ser Leu Pro Leu Leu Arg Lys Gly Ser Leu Arg Asn Pro Val Val Glu 
        675                 680                 685             


Lys Ile Leu Asn Gln Met Thr His Val Ile Asn Thr His Ile Ser Glu 
    690                 695                 700                 


Tyr Gly Asn Pro Asp Glu Ile Arg Ile Glu Met Ala Arg Asp Met Gln 
705                 710                 715                 720 


Ala Asn Ser Lys Gln Arg Lys Ala Met Thr Thr Ala Ile Ala Glu Ala 
                725                 730                 735     


Thr Lys Arg Asn Thr Gln Phe Ile Glu Glu Ile Gln Lys Glu Phe Gly 
            740                 745                 750         


Phe Asn Pro Ser Lys Asn Asp Leu Ile Lys Tyr Lys Leu Trp Lys Glu 
        755                 760                 765             


Leu Ala Pro Leu Gly Ser Lys Thr Leu Tyr Ser Asn Thr Tyr Ile Pro 
    770                 775                 780                 


Lys Glu Glu Leu Phe Thr Ala Lys Phe Glu Ile Glu His Ile Ile Pro 
785                 790                 795                 800 


Arg Ala Leu Phe Tyr Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu Leu 
                805                 810                 815     


Asn Ser Val Asn Lys Leu Lys Gly Asn Lys Thr Ala Leu Asp Tyr Met 
            820                 825                 830         


Asp Thr Leu Ser Gln Glu Gln Ala Asn Asp Tyr Ile Ile Arg Val Glu 
        835                 840                 845             


Glu Leu Lys Lys Ile Gly Ala Ile Thr Gln Ala Lys Tyr Lys Lys Leu 
    850                 855                 860                 


Leu Ile Arg Glu Lys Asp Leu Gly Asp Asp Phe Thr Asn Arg Asp Leu 
865                 870                 875                 880 


Thr Asn Thr Arg Tyr Ile Ile Arg His Ala Ala Asp Met Leu Leu Gln 
                885                 890                 895     


Val Thr Pro Ser Ile Val His Thr Asn Gly Ser Ile Thr Ala Ile Leu 
            900                 905                 910         


Arg Arg Asp Trp Glu Leu Leu Asp Ile Leu Lys Glu Leu Asn Leu Pro 
        915                 920                 925             


Thr Tyr Glu Ser Ile Asp Arg Lys Ile Ser Asp Pro Lys Glu Lys Leu 
    930                 935                 940                 


Thr Glu Ser Val Glu Arg Lys Asp Gly Gln Ala Ile Lys Met Ile Lys 
945                 950                 955                 960 


Asp Trp Ser Lys Arg Asp Asp His Arg His His Ala Met Asp Ala Leu 
                965                 970                 975     


Ile Thr Ala Leu Thr Thr Arg Lys His Val Gln Tyr Leu Asn Gly Asn 
            980                 985                 990         


Arg Gly Ala Glu Glu Thr Asp Lys  Val Gln Lys Leu Gln  Gln Leu Lys 
        995                 1000                 1005             


Ala Leu  Arg Lys Gln Leu Ile  Gly Ser Asp Gly His  Phe Ile Pro 
    1010                 1015                 1020             


Pro Met  His Asp Ile Arg Val  Gln Ala Lys Glu Ala  Leu Ser Arg 
    1025                 1030                 1035             


Val Ile  Ile Ser Thr Lys Ala  Lys Gly Lys Val Thr  Thr Pro Asn 
    1040                 1045                 1050             


Thr Asn  Ile Ile Cys Ser Arg  Asp Lys Thr Thr Gly  Arg Lys Ile 
    1055                 1060                 1065             


Tyr Val  Lys Gln Thr Thr Leu  Thr Pro Arg Gly Ala  Leu His Lys 
    1070                 1075                 1080             


Glu Met  Gln Tyr Gly Cys Asn  His Tyr Tyr Leu Glu  Lys Gln Ile 
    1085                 1090                 1095             


Pro Ile  Gly Ser Lys Leu Thr  Glu Glu Glu Ile Arg  Leu Val Ser 
    1100                 1105                 1110             


Ser Pro  Lys Ile Arg Ser Met  Leu Ser Glu Arg Leu  Gln Ala Tyr 
    1115                 1120                 1125             


Asn Asn  Asp Pro Lys Ser Ala  Phe Thr Gly Arg Asn  Ser Met Asp 
    1130                 1135                 1140             


Lys Lys  Pro Leu Glu Asp Lys  Asn Lys Lys Leu Val  Tyr Thr Asp 
    1145                 1150                 1155             


Lys Lys  Val Lys Gln Thr Ile  Leu Ala Asn Arg Phe  Val Lys Arg 
    1160                 1165                 1170             


Asp Arg  Val Asn Ser Lys Leu  Asp Leu Asp Lys Val  Ile Asp Thr 
    1175                 1180                 1185             


Gly Ile  Lys Arg Ile Leu Glu  Arg Arg Leu Lys Gln  Tyr Gly Gly 
    1190                 1195                 1200             


Lys Tyr  Glu Thr Ala Phe Ser  Asp Leu Glu Asn Asn  Pro Ile Trp 
    1205                 1210                 1215             


Leu Asn  Glu Glu Lys Gly Ile  Ala Ile Lys Ser Val  Arg Ile Arg 
    1220                 1225                 1230             


Gly Lys  Asn Asn Thr Ile Thr  Ile Arg Gln Thr Asn  Thr Cys Arg 
    1235                 1240                 1245             


Ile Lys  Tyr Val Ala Asn Asp  Asn Asn His His Leu  Ala Val Tyr 
    1250                 1255                 1260             


Ala Leu  Pro Asp Gly Ser Ile  Glu Asp Asp Ile Val  Ser Phe Phe 
    1265                 1270                 1275             


Asp Ala  Val Glu Arg Ser Arg  Lys Gly Leu Pro Ile  Ile Asp Lys 
    1280                 1285                 1290             


His Lys  Asn Gln Ser Leu Gly  Trp Lys Phe Leu Phe  Ser Val Lys 
    1295                 1300                 1305             


Gln Asn  Glu Tyr Phe Leu Leu  Pro Gln Gln Gly Glu  Lys Ile Asp 
    1310                 1315                 1320             


Ile Asp  Thr Gly Glu Ile Ser  Met Gln Thr Thr Phe  Ser Pro Lys 
    1325                 1330                 1335             


Asp Leu  Ser Pro Glu Tyr Ile  Met Asn Pro Asp Asn  Tyr Ala Glu 
    1340                 1345                 1350             


Leu Ala  Pro His Ile Phe Arg  Val Gln Ser Ile Ser  Lys Thr Pro 
    1355                 1360                 1365             


Gln Gly  Asn Arg Asp Val Arg  Ile Tyr Leu Phe Lys  Gln Gln Tyr 
    1370                 1375                 1380             


Ile Ser  Gly Ser Gln Lys Asp  Ser Leu His Leu Lys  Gly Ile Thr 
    1385                 1390                 1395             


His Arg  Gln Ile Ser Ser Ala  Lys Asp Leu Ala Gly  Leu Val Lys 
    1400                 1405                 1410             


Ile Arg  Val Asn His Leu Gly  Arg Ile Val Ala Val  Gly Glu Lys 
    1415                 1420                 1425             


<210>  1230
<211>  1428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1230

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Ala Ser Ile Gly Ser 
1               5                   10                  15      


Ala Tyr Val Leu Glu Ala Glu Lys Ser Ala Asp Gln Thr Lys Ile Val 
            20                  25                  30          


Tyr Leu Gly Thr Arg Ile Ile Leu Ser Ser Ala Asp Ala Glu Asn Phe 
        35                  40                  45              


Ser Lys Gly Leu Ser Cys Ser Pro Thr Thr Asp Arg Thr Phe Lys Arg 
    50                  55                  60                  


Ser Met Arg Arg Arg Leu Gln Arg Tyr Lys Gln Arg Arg Ser Ser Leu 
65                  70                  75                  80  


Ile Gln Gln Phe Lys Lys Ile Gly Ile Ile Thr Asn Thr Glu Gln Ile 
                85                  90                  95      


Ile Arg Glu Pro Ile Ser Glu Thr His Glu Leu Leu Arg Ile Arg Ser 
            100                 105                 110         


Lys Ala Val Ser Glu Arg Val Ser Leu Glu Glu Phe Ala Arg Ile Leu 
        115                 120                 125             


Leu Thr Ile Asn Lys Arg Arg Gly Tyr Lys Ser Asn Arg Lys Thr Ser 
    130                 135                 140                 


Lys Ser Thr Glu Glu Asp Gly Lys Leu Val Asp Ala Met Ala Val Thr 
145                 150                 155                 160 


Lys Glu Leu Ile Lys Leu Lys Lys Thr Pro Gly Gln Tyr Met Phe Glu 
                165                 170                 175     


Arg Phe Lys Arg Gly Lys Tyr Ala Glu Ile Asp Phe Tyr Pro Phe Asp 
            180                 185                 190         


Leu Ile Asn Glu Leu Lys Gln Ile Trp Glu Val Gln Gly Gln Lys Tyr 
        195                 200                 205             


Pro Asp Val Leu Thr Glu Asp Leu Leu Ser Ser Leu Leu Lys Gln Lys 
    210                 215                 220                 


Thr Gly Lys Ala Lys Glu Leu Ile Tyr Ser Lys Ser Asp Leu Asn Pro 
225                 230                 235                 240 


Ala Glu Val Pro Ile Asp Tyr Gln Lys Gly Lys Lys Leu Arg Lys Thr 
                245                 250                 255     


Ile Leu Asn Lys Tyr Phe Ala Tyr Glu Ser Arg Ser Leu Ala Val Thr 
            260                 265                 270         


Asn Ile Ile Pro Val Glu Gln Ala Ile Thr Ala Ile Val Ala Ile Cys 
        275                 280                 285             


Lys Gln Ile Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Gly Asp Arg 
    290                 295                 300                 


Ser Arg Val Leu Tyr Ala Asn Asn Gln Thr Ile Gly Gln Tyr Leu Tyr 
305                 310                 315                 320 


Glu Arg Ile Lys Ala Asn Pro His Asp Arg Ile Arg Gly Glu Val Phe 
                325                 330                 335     


Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Asp Arg Ile Trp Glu Cys Gln 
            340                 345                 350         


Gln Asn Tyr Tyr Pro Ala Ile Leu Thr Asn Asp Arg Lys Gln Tyr Ile 
        355                 360                 365             


Arg Asn Arg Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser 
    370                 375                 380                 


Glu Leu Ala Phe Cys Glu Phe Glu Ser Arg Glu Val Glu His Asn Gly 
385                 390                 395                 400 


Lys Lys Tyr Thr Glu Gly Leu His Val Ala Pro Lys Ser Ser Pro Val 
                405                 410                 415     


Phe Gln His Phe Arg Leu Trp Gln Arg Leu Gly Gln Ile Glu Val Ser 
            420                 425                 430         


Pro Leu Arg Lys Trp Asp Lys Gln Ser Asn Glu Leu Phe Glu Gln Gln 
        435                 440                 445             


Asn Asn Ala Asp Arg Glu Gly Ile Leu Asn Thr Glu Ala Lys Gln Lys 
    450                 455                 460                 


Leu Phe Ser Tyr Leu Asn Leu Leu Asp His Leu Thr Asp Asn Glu Ile 
465                 470                 475                 480 


Leu Asn Ile Leu Gly Tyr Ser Pro Asp Lys Trp Lys Ile Asn Phe Arg 
                485                 490                 495     


Glu Ile Ile Gly Asn Arg Thr Asn Asn Leu Met Met Lys Ala Phe Phe 
            500                 505                 510         


Ser Met Lys Glu Met Thr Asp Gly Glu Gln Ile Asp Trp Glu Lys Asn 
        515                 520                 525             


Leu Thr Lys Ile Thr Asn Gly Gln Ser Leu Ile Ser Pro Glu Gln Ile 
    530                 535                 540                 


Val Asp Leu Ile Arg Gln Phe Phe Glu Arg Glu Gly Ile Thr Thr Ser 
545                 550                 555                 560 


Leu Leu Tyr Phe Asp Pro Ala Thr Asp Asn Phe Arg Glu Gln Ser His 
                565                 570                 575     


Tyr Ser Leu Trp His Leu Leu Tyr Ser Tyr Glu Asp Asp Asn Ser Pro 
            580                 585                 590         


Thr Gly Thr Thr Ser Leu Cys Lys Thr Leu Ala Lys Arg Tyr Gly Ile 
        595                 600                 605             


Pro His Glu Leu Cys Pro Leu Leu Ala Ser Leu Thr Phe Glu Arg Gln 
    610                 615                 620                 


Tyr Gly Ser Leu Ser Thr Lys Ala Met Arg Lys Leu Leu Pro Phe Met 
625                 630                 635                 640 


Gln Glu Lys Arg Tyr Asp Glu Ala Cys Glu Leu Ala Gly Tyr Arg His 
                645                 650                 655     


Ser Ala Trp Ile Thr Lys Glu Glu Asn Ala Lys Arg Thr Leu Lys Asn 
            660                 665                 670         


Lys Leu Pro Leu Leu Arg Lys Gly Ser Leu Arg Asn Pro Val Val Glu 
        675                 680                 685             


Lys Ile Leu Asn Gln Met Thr His Val Ile Asn Thr His Ile Ala Glu 
    690                 695                 700                 


Tyr Gly Asn Pro Asp Glu Ile Arg Ile Glu Met Ala Arg Asp Met Gln 
705                 710                 715                 720 


Ala Asn Ser Lys Gln Arg Lys Ala Met Thr Ala Ala Ile Thr Glu Ala 
                725                 730                 735     


Thr Lys Arg Asn Thr Gln Phe Ile Glu Glu Ile Gln Lys Glu Phe Gly 
            740                 745                 750         


Phe Asn Pro Ser Lys Asn Asp Leu Ile Lys Tyr Lys Leu Trp Lys Glu 
        755                 760                 765             


Leu Ala Pro Leu Gly Asn Lys Thr Leu Tyr Ser Asn Ile Tyr Ile Pro 
    770                 775                 780                 


Lys Glu Glu Leu Phe Thr Ala Lys Phe Glu Ile Glu His Ile Ile Pro 
785                 790                 795                 800 


Arg Ala Leu Phe Tyr Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu Leu 
                805                 810                 815     


Asn Ser Val Asn Lys Leu Lys Gly Asn Lys Thr Ala Leu Asp Tyr Leu 
            820                 825                 830         


Asp Thr Leu Gly Gln Glu Lys Ala Asn Asp Tyr Ile Leu Arg Val Glu 
        835                 840                 845             


Glu Leu Lys Lys Ile Gly Ala Ile Thr Gln Ala Lys Tyr Lys Lys Leu 
    850                 855                 860                 


Leu Ile Arg Glu Lys Asp Leu Gly Asp Asp Phe Thr Asn Arg Asp Leu 
865                 870                 875                 880 


Thr Asn Thr Arg Tyr Ile Ile Arg His Ala Ala Asp Met Leu Leu Gln 
                885                 890                 895     


Val Thr Pro Ser Ile Val His Thr Asn Gly Ser Ile Thr Ala Ile Leu 
            900                 905                 910         


Arg Arg Asp Trp Gly Leu Leu Asp Ile Leu Lys Glu Leu Asn Leu Pro 
        915                 920                 925             


Thr Tyr Glu Ser Ile Asp Arg Lys Ile Ser Asp Pro Glu Glu Lys Leu 
    930                 935                 940                 


Thr Gln Ser Val Asp Arg Lys Asp Gly Gln Ala Ile Lys Met Ile Lys 
945                 950                 955                 960 


Asp Trp Ser Lys Arg Asp Asp His Arg His His Ala Met Asp Ala Leu 
                965                 970                 975     


Ile Thr Ala Leu Thr Thr Arg Lys His Val Gln Tyr Leu Asn Gly Asn 
            980                 985                 990         


Ser Gly Ala Glu Glu Thr Asp Lys  Ala Gln Lys Leu Glu  Gln Leu Lys 
        995                 1000                 1005             


Ala Leu  Arg Lys Gln Leu Ile  Gly Ser Asp Gly His  Phe Ile Pro 
    1010                 1015                 1020             


Pro Met  His Asp Ile Arg Val  Gln Ala Lys Glu Ala  Leu Ser Arg 
    1025                 1030                 1035             


Val Ile  Ile Ser Thr Lys Val  Lys Gly Lys Val Thr  Thr Pro Asn 
    1040                 1045                 1050             


Thr Asn  Ile Ile Cys Ser Arg  Asp Lys Thr Thr Gly  Arg Lys Ile 
    1055                 1060                 1065             


Tyr Val  Arg Gln Thr Thr Leu  Thr Pro Arg Gly Ala  Leu His Leu 
    1070                 1075                 1080             


Asp Thr  Lys Tyr Gly Ile Asn  Arg Tyr Tyr Gln Glu  Arg Glu Val 
    1085                 1090                 1095             


Ser Ile  Gly Ser Lys Leu Thr  Gln Glu Glu Ile Arg  Leu Val Ser 
    1100                 1105                 1110             


Ser Pro  His Ile Gln Ser Ile  Leu Leu Glu Arg Leu  Arg Ala Phe 
    1115                 1120                 1125             


Asp Asn  Asn Pro Lys Ser Ala  Phe Thr Gly Arg Asn  Ser Val Asp 
    1130                 1135                 1140             


Lys Lys  Pro Leu Glu Asp Lys  Asn Lys Lys Leu Val  Tyr Thr Asp 
    1145                 1150                 1155             


Lys Lys  Val Lys Gln Thr Ile  Leu Ala Asn Arg Phe  Val Lys Arg 
    1160                 1165                 1170             


Asp Gln  Val Asn Ser Lys Leu  Asn Leu Asp Lys Val  Ile Asp Thr 
    1175                 1180                 1185             


Gly Ile  Lys Arg Ile Leu Glu  Gly Arg Leu Gln Gln  Tyr Gly Gly 
    1190                 1195                 1200             


Lys Tyr  Glu Thr Ala Phe Ser  Asp Leu Glu Asn Asn  Pro Ile Trp 
    1205                 1210                 1215             


Leu Asn  Glu Glu Lys Gly Ile  Ala Ile Lys Ser Val  Arg Ile Arg 
    1220                 1225                 1230             


Gly Lys  Asn Asn Ala Ile Pro  Ile His Gln Thr Asp  Thr Cys Pro 
    1235                 1240                 1245             


Ile Asp  Tyr Val Ala Asn Asp  Asn Asn His His Leu  Ala Val Tyr 
    1250                 1255                 1260             


Val Leu  Pro Asp Gly Thr Ile  Glu Asp Asp Ile Val  Thr Phe Phe 
    1265                 1270                 1275             


Asp Ala  Val Glu Arg Ser Arg  Gln Gly Leu Pro Ile  Val Asp Lys 
    1280                 1285                 1290             


Asn Lys  Asn Gln Ser Leu Gly  Trp Lys Phe Leu Phe  Ser Ile Lys 
    1295                 1300                 1305             


Gln Asn  Glu Tyr Phe Leu Leu  Pro Gln Gln Ala Glu  Lys Ile Asp 
    1310                 1315                 1320             


Ile Asp  Thr Gly Glu Ile Ser  Thr Gln Thr Thr Phe  Ser Pro Lys 
    1325                 1330                 1335             


Asp Leu  Ser Pro Glu Tyr Ile  Met Asn Pro Asp Asn  Tyr Ala Glu 
    1340                 1345                 1350             


Leu Ala  Ser His Ile Phe Arg  Val Gln Ser Ile Ser  Lys Thr Pro 
    1355                 1360                 1365             


Gln Gly  Asn Arg Asp Val Arg  Ile Tyr Leu Phe Lys  Gln Gln Tyr 
    1370                 1375                 1380             


Ile Ser  Gly Ser Gln Lys Asp  Ser Leu His Leu Lys  Gly Ile Thr 
    1385                 1390                 1395             


Tyr Arg  Gln Ile Ser Ser Ala  Lys Asp Leu Ala Glu  Leu Val Lys 
    1400                 1405                 1410             


Ile Arg  Val Asn His Leu Gly  Arg Ile Val Ser Val  Gly Glu Lys 
    1415                 1420                 1425             


<210>  1231
<211>  1428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1231

Met Thr Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly 
1               5                   10                  15      


Trp Ala Tyr Val Ile Glu Ser Asp Asn Pro Asp Glu Ser Glu Ile Lys 
            20                  25                  30          


Gln Ile Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Gln Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Pro Phe Ser Ile Asn Lys Glu Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Asn Leu Asp Arg Tyr Lys Asp Arg Arg Asn Asn 
65                  70                  75                  80  


Leu Ile Glu Val Leu Lys Lys Ala Asn Ile Leu Ser Gln Asp Ser Ile 
                85                  90                  95      


Leu Thr Glu Glu Gly Gln His Thr Thr His Gln Thr Trp Gln Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Val Asp Lys Ile Glu Lys Glu Glu Leu Ala Arg Val 
        115                 120                 125             


Leu Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Glu Asp Glu Gly Gln Ala Ile Asp Gly Met Ala Val Ala Lys 
145                 150                 155                 160 


Arg Leu Tyr Asp Glu Gln Ile Thr Cys Gly Gln Leu Val Tyr Gln Leu 
                165                 170                 175     


Leu Arg Glu Gly Lys Lys Ser Ile Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asp Lys Val Trp Asn Phe Gln Lys Gln Phe Tyr Pro 
        195                 200                 205             


Lys Ile Leu Thr Asp Glu Phe Tyr Lys Glu Leu Lys Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ser Ala Leu Phe Tyr Lys Thr Tyr Asn Phe Asn Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Ser Arg Glu Glu Lys Lys Ile Gln Ala Tyr Lys Trp 
                245                 250                 255     


Arg Ser Glu Ala Ile Ser Gln Gln Leu Glu Lys Glu Glu Val Ala Phe 
            260                 265                 270         


Val Leu Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr 
    290                 295                 300                 


Val Gly Gln Tyr Leu Tyr Gln Gln Leu Gln Glu Asn Arg His Ala Arg 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Ala Ile Trp Lys Glu Gln Ala Lys His His Thr Glu Leu Thr Glu Glu 
            340                 345                 350         


Leu Lys Ala Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Arg Glu 
    370                 375                 380                 


Ile Gln Ile Asp Gly Lys Lys Lys Thr Ile Gly Leu Arg Val Ala Pro 
385                 390                 395                 400 


Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Asn Leu Asn 
                405                 410                 415     


Asn Val Leu Val Arg Lys Lys Gly Ser Arg Lys Arg Asn Val Lys Gln 
            420                 425                 430         


Glu Ile Gln Gly Ser Leu Phe Glu Glu Glu Lys Glu Ile Phe Val Phe 
        435                 440                 445             


Asp Leu Glu Thr Lys Glu Lys Leu Phe Glu Glu Leu Asn Leu Lys Gly 
    450                 455                 460                 


Asn Leu Ser Ala Asn Lys Val Ile Glu Leu Leu Gly His Lys Pro Thr 
465                 470                 475                 480 


Glu Trp Glu Ile Asn Tyr Ser Ala Leu Glu Gly Asn Arg Thr Asn Gln 
                485                 490                 495     


Ala Leu Tyr Asn Ala Tyr Leu Lys Ile Leu Asp Ile Glu Gly Tyr Asp 
            500                 505                 510         


Glu Asp Leu Leu Lys Leu Ser Asp Lys Asp Asp Ile Asn Val Ala Asp 
        515                 520                 525             


Leu Lys Thr Pro Ala Ser Glu Ile Lys Asn Met Val Arg Asp Ile Phe 
    530                 535                 540                 


Gln Val Leu Gly Ile Ser Thr Asp Ile Leu Glu Phe Asp Ala Glu Leu 
545                 550                 555                 560 


Asp Gly Lys Asp Phe Glu Gln Gln Ala Ser Tyr Gln Leu Trp His Leu 
                565                 570                 575     


Leu Tyr Ser Tyr Glu Gly Asp Asp Ser Gln Ser Gly Asn Glu Lys Leu 
            580                 585                 590         


Tyr Glu Leu Leu Glu Lys Lys Phe Gly Phe Lys Lys Glu His Ser Gln 
        595                 600                 605             


Val Leu Ala Asn Val Thr Leu Ser Asp Asp Tyr Gly Asn Leu Ser Ser 
    610                 615                 620                 


Lys Ala Met Arg Lys Ile Tyr Pro Phe Ile Lys Glu Asn Asn Tyr Ser 
625                 630                 635                 640 


Thr Ala Cys Glu Leu Ala Gly Tyr Arg His Ser Lys His Ser Leu Thr 
                645                 650                 655     


Lys Glu Glu Ile Glu Asn Arg Pro Leu Asp Asp Lys Leu Glu Leu Leu 
            660                 665                 670         


Lys Lys Asn Ser Leu Arg Gln Pro Val Val Glu Lys Ile Leu Asn Gln 
        675                 680                 685             


Met Val Asn Val Val Asn Ser Leu Leu Glu Lys Asn Arg Lys Glu Asn 
    690                 695                 700                 


Pro Asp Phe Arg Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu 
705                 710                 715                 720 


Lys Lys Asn Ala Lys Glu Arg Ala Glu Met Thr Thr Asn Ile Asn Ser 
                725                 730                 735     


Ala Lys Thr Glu Asn Glu Lys Ile Phe Lys Leu Leu Gln Ala Glu Phe 
            740                 745                 750         


Gly Leu Lys Asn Pro Thr Arg Asn Asp Ile Ile Arg Tyr Arg Leu Tyr 
        755                 760                 765             


Glu Glu Leu Lys Asn Asn Gly Tyr Lys Asp Leu Tyr Thr Asn Lys Tyr 
    770                 775                 780                 


Ile Pro Arg Glu Ile Leu Phe Ser Lys Gln Val Asp Ile Glu His Ile 
785                 790                 795                 800 


Ile Pro Gln Ser Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Ile 
                805                 810                 815     


Val Tyr Arg Glu Thr Asn Leu Asp Lys Gly Asn Gln Thr Ala Phe Asp 
            820                 825                 830         


Tyr Ile Asp Ser Lys Tyr Gly Asn Glu Lys Val Glu Glu Tyr Ile Leu 
        835                 840                 845             


Arg Ile Glu Ala Leu His Asp Ala Trp Lys Lys Asn Lys Glu Glu Gly 
    850                 855                 860                 


Ile Ser Lys Ala Lys Tyr Gln Lys Leu Leu Lys Lys Glu Thr Glu Ile 
865                 870                 875                 880 


Gly Asp Gly Phe Ile Glu Arg Asp Leu Arg Glu Ser Gln Tyr Ile Ala 
                885                 890                 895     


Lys Lys Ala Lys Glu Met Leu Phe Lys Ile Thr Arg Ser Val Val Ser 
            900                 905                 910         


Thr Ser Gly Ser Ile Thr Asp Lys Leu Arg Glu Asp Trp Gly Leu Ile 
        915                 920                 925             


Asn Ile Met Lys Glu Leu Asn Leu Pro Lys Tyr Arg Ser Leu Gly Leu 
    930                 935                 940                 


Thr Glu Met Gln Glu Arg Lys Phe Gly Gln Lys Val Glu Val Ile Asn 
945                 950                 955                 960 


Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu 
                965                 970                 975     


Thr Val Ala Phe Thr Lys His Asn His Ile Gln Tyr Leu Asn Tyr Leu 
            980                 985                 990         


Asn Ala Arg Lys Asn Glu Asn His  Lys Leu His Asn Asn  Ile Ile Ala 
        995                 1000                 1005             


Ile Glu  Asn Leu Glu Thr Glu  Thr Met Ile Asp Asn  Asn Gly Ser 
    1010                 1015                 1020             


Lys Arg  Arg Val Phe Lys Ala  Pro Met Pro Asn Phe  Arg Gln Val 
    1025                 1030                 1035             


Ala Lys  Glu His Leu Glu Asn  Val Leu Ile Ser His  Lys Ala Lys 
    1040                 1045                 1050             


Asn Lys  Val Val Thr Lys Asn  Lys Asn Lys Ile Ala  Gly Lys Val 
    1055                 1060                 1065             


Ala Ala  Gln Glu Thr Leu Thr  Pro Arg Gly Gln Leu  His Lys Glu 
    1070                 1075                 1080             


Thr Val  Tyr Gly Lys Tyr Gln  Tyr Tyr Val Asn Lys  Glu Glu Lys 
    1085                 1090                 1095             


Ile Gly  Thr Lys Phe Asp Glu  Glu Thr Ile Asn Lys  Val Ser Asn 
    1100                 1105                 1110             


Pro Gln  Tyr Arg Lys Leu Leu  Leu Glu Arg Leu Ser  Ala Asn Gly 
    1115                 1120                 1125             


Asn Asp  Pro Lys Lys Ala Phe  Ser Gly Lys Asn Ala  Leu Ser Lys 
    1130                 1135                 1140             


Asn Pro  Ile Tyr Leu Asn Glu  Thr Lys Thr Glu Thr  Val Pro Glu 
    1145                 1150                 1155             


Val Val  Lys Leu Thr Trp Leu  Glu Glu Asp Tyr Ser  Ile Arg Lys 
    1160                 1165                 1170             


Asp Ile  Thr Pro Asp Asn Phe  Lys Asp Glu Lys Ser  Ile Asp Lys 
    1175                 1180                 1185             


Ile Leu  Asp Glu Gly Val Lys  Arg Ile Leu Lys Lys  Arg Leu Arg 
    1190                 1195                 1200             


Lys Asn  Asp Asn Asp Pro Lys  Lys Ala Phe Ser Asp  Leu Asp Lys 
    1205                 1210                 1215             


Asn Pro  Ile Trp Leu Asn Glu  Glu Lys Gly Ile Ser  Ile Lys Arg 
    1220                 1225                 1230             


Val Thr  Ile Ser Gly Val Asn  Asn Ala Glu Pro Leu  His Tyr Lys 
    1235                 1240                 1245             


Lys Asp  His Phe Gly Asn Asp  Ile Leu Asp Glu Lys  Gly Asp Lys 
    1250                 1255                 1260             


Ile Pro  Val Asp Phe Val Ser  Thr Gly Asn Asn His  His Val Ala 
    1265                 1270                 1275             


Val Tyr  Arg Asp Glu Lys Gly  Asn Leu Gln Glu Asn  Ile Val Ser 
    1280                 1285                 1290             


Phe Phe  Glu Ala Val Ile Arg  Val Asn Gln Gly Leu  Pro Ile Ile 
    1295                 1300                 1305             


Asp Lys  Ala Tyr Asn Gln His  Leu Gly Trp Gln Phe  Leu Phe Thr 
    1310                 1315                 1320             


Met Lys  Gln Asn Glu Met Phe  Val Phe Pro Asn Glu  Lys Ser Gly 
    1325                 1330                 1335             


Phe Asn  Pro Gln Glu Ile Asp  Leu Leu Asp Pro Ser  Asn Lys Lys 
    1340                 1345                 1350             


Lys Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Met  Ser Lys Val 
    1355                 1360                 1365             


Ile Tyr  Gly Asn Ser Pro Val  Arg Glu Tyr Val Phe  Arg His His 
    1370                 1375                 1380             


Leu Glu  Thr Asn Val Asp Glu  Lys Lys Glu Leu Lys  Asp Ile Thr 
    1385                 1390                 1395             


Phe Lys  Asn Ile Lys Ser Leu  Gly Tyr Phe Glu Asp  Ile Val Lys 
    1400                 1405                 1410             


Val Arg  Ile Asn His Leu Gly  Asp Ile Val Ser Val  Gly Glu Tyr 
    1415                 1420                 1425             


<210>  1232
<211>  1430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1232

Met Lys His Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Lys Glu Ala Gln Asn Asp Asn Glu Gln Ser Glu Ile Val 
            20                  25                  30          


Asp Val Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Pro Ile Ser Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Lys Glu 
65                  70                  75                  80  


Leu Ile Glu Leu Leu Lys Glu His Gln Leu Ile Ser Thr Asp Thr Ile 
                85                  90                  95      


Leu Thr Glu Asn Gly Lys Gly Thr Thr Phe Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Lys Glu Lys Ile Ser Leu Glu Glu Leu Ala Arg Val 
        115                 120                 125             


Phe Leu Thr Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Glu Asp Glu Gly Gln Leu Ile Asp Gly Met Glu Ile Ala Lys 
145                 150                 155                 160 


Arg Leu Tyr Glu Glu Asn Leu Thr Thr Gly Gln Leu Ser Leu Gln Leu 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Ser Leu Pro Asp Tyr Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Phe Glu Leu Asp Lys Val Trp Asn Phe Gln Lys Gln Phe His Ser 
        195                 200                 205             


Asp Ile Leu Thr Asp Glu Phe Tyr Ala Gln Leu Lys Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ser Ala Leu Phe Leu Ala Lys Tyr Gln Ile Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Ser Arg Glu Asp Lys Lys Leu Gln Ala Tyr Gln Trp 
                245                 250                 255     


Arg Val Asp Ala Leu Ser Lys Glu Leu Thr Arg Glu Gln Leu Ala Phe 
            260                 265                 270         


Val Ile Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Glu Thr 
    290                 295                 300                 


Val Gly Glu Tyr Leu Trp Lys Gln Ile Ala Ala Asn Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Lys Thr Gln Ser Ala Phe Tyr Pro Glu Ile Leu Thr Asp 
            340                 345                 350         


Glu Leu Lys Lys Glu Leu Arg Asp Val Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Ser Leu Val Ser Phe Cys Glu Phe Glu Asn Lys 
    370                 375                 380                 


Glu Ile Glu Ile Lys Lys Glu Asp Gly Thr Ser Thr Lys Lys Val Ile 
385                 390                 395                 400 


Gly Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys 
                405                 410                 415     


Ile Trp Gln Asn Leu Asn Asn Val Leu Ile Arg Lys Lys Gly Ser Lys 
            420                 425                 430         


Thr Lys Lys Val Ala Lys Asn Gln Gln Ala Val Leu Phe Glu Glu Glu 
        435                 440                 445             


Lys Thr Ile Ser Glu Leu Asp Leu Glu Ala Lys Gln Ala Leu Phe Asn 
    450                 455                 460                 


Glu Leu Asn Leu Lys Gly Asn Leu Lys Ala Asp Tyr Cys Leu Glu Ile 
465                 470                 475                 480 


Leu Gly Tyr Ser Pro Lys Glu Trp Glu Met Asn Tyr Ser Val Leu Glu 
                485                 490                 495     


Gly Asn Arg Thr Asn Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Thr 
            500                 505                 510         


Asp Leu Glu Gly Tyr Asp Ala Lys Asn His Leu Lys Val Lys Leu Asn 
        515                 520                 525             


Lys Asp Glu Val Glu Leu Asp Asp Leu Lys Val Pro Ala Ser Glu Ile 
    530                 535                 540                 


Lys Asp Met Val Gln Ala Ile Phe Lys Glu Ala Gly Ile Asn Thr Asp 
545                 550                 555                 560 


Ile Leu His Phe Asn Ala Glu Leu Asp Gly Lys Asp Phe Glu Asn Gln 
                565                 570                 575     


Ala Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp 
            580                 585                 590         


Ser Lys Ser Gly Asn Glu Leu Leu Tyr Lys Leu Leu Glu Thr Lys Phe 
        595                 600                 605             


Gly Phe Lys Lys Glu Tyr Ala Gln Ile Leu Ala Asn Val Ser Leu Ser 
    610                 615                 620                 


Asp Asp Tyr Gly Ser Leu Ser Thr Lys Ala Met Arg Lys Ile His Pro 
625                 630                 635                 640 


Tyr Ile Lys Glu Asn Lys Phe Ser Thr Ala Cys Glu Leu Ala Gly Tyr 
                645                 650                 655     


Arg His Ser Lys His Ser Leu Thr Lys Glu Glu Asn Asp Asn Arg Val 
            660                 665                 670         


Leu Lys Asp Ser Leu Glu Val Leu Lys Lys Asn Ser Leu Arg Gln Pro 
        675                 680                 685             


Val Val Glu Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Ala Leu 
    690                 695                 700                 


Leu Lys Lys Tyr Arg Thr Glu Asn Pro Asn Phe Lys Phe Asp Glu Ile 
705                 710                 715                 720 


Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Glu Glu Arg Ala 
                725                 730                 735     


Asn Met Thr Ala Asn Ile Asn Lys Ala Lys Thr Glu His Glu Lys Ile 
            740                 745                 750         


Ile Lys Ile Leu Lys Asn Glu Phe Gly Leu Pro Asn Pro Thr Arg Asn 
        755                 760                 765             


Asp Ile Ile Arg Tyr Arg Leu Tyr Glu Glu Leu Lys Ala Asn Gly Tyr 
    770                 775                 780                 


Lys Asp Leu Tyr Thr Asn Ile Lys Ile Pro Gln Glu Lys Leu Phe Thr 
785                 790                 795                 800 


Lys Glu Ile Asp Ile Glu His Ile Ile Pro Lys Ser Arg Val Phe Asp 
                805                 810                 815     


Asp Ser Phe Ser Asn Lys Thr Leu Ser Phe Arg Lys Thr Asn Leu Asp 
            820                 825                 830         


Lys Gly Glu Arg Thr Ala Phe Asp Tyr Met Glu Ser Lys Phe Gly Ala 
        835                 840                 845             


Asp Lys Leu Glu Glu Tyr Val Ala Arg Val Glu Asp Leu Tyr Lys Asn 
    850                 855                 860                 


Asn Ile Ile Ser Lys Ala Lys Tyr Gln Lys Leu Leu Lys Lys Glu Ser 
865                 870                 875                 880 


Glu Ile Gly Asp Gly Phe Val Glu Arg Asp Leu Arg Glu Thr Gln Tyr 
                885                 890                 895     


Ile Ala Lys Lys Ala Lys Glu Ile Leu Leu Gln Ile Thr Pro Asn Val 
            900                 905                 910         


Leu Ser Thr Ser Gly Gly Ile Thr Asp Arg Leu Arg Glu Asp Trp Asp 
        915                 920                 925             


Leu Ile Asn Val Met Lys Glu Leu Asn Leu Pro Lys Tyr Arg Ala Leu 
    930                 935                 940                 


Gly Leu Thr Gln Met Glu Asp Arg Lys Tyr Gly Gln Gln Val Glu Val 
945                 950                 955                 960 


Ile Thr Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp 
                965                 970                 975     


Ala Leu Thr Val Ala Phe Thr Lys His Asn His Ile Gln Tyr Leu Asn 
            980                 985                 990         


Tyr Leu Asn Ala Arg Lys Asn Glu  Lys His Lys Glu His  Ser Asn Ile 
        995                 1000                 1005             


Met Gly  Ile Gln Gln Leu Glu  Thr Ile Lys Val Thr  Asp Lys Asn 
    1010                 1015                 1020             


Gly Asn  Glu Lys Arg Val Phe  Lys Ala Pro Met Pro  Asn Phe Arg 
    1025                 1030                 1035             


Gln Val  Thr Lys Ala Phe Leu  Glu Asn Ile Leu Ile  Ser His Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Val Thr  Lys Asn Lys Asn Lys  Val Ala Gly 
    1055                 1060                 1065             


Ser Asn  Lys Val Gln Glu Val  Leu Thr Pro Arg Gly  Gln Leu His 
    1070                 1075                 1080             


Lys Glu  Thr Val Tyr Gly Lys  Tyr Gln Tyr Tyr Val  Gln Lys Glu 
    1085                 1090                 1095             


Glu Lys  Ile Gly Ala Lys Phe  Asp Leu Glu Thr Ile  Lys Lys Val 
    1100                 1105                 1110             


Ala Asn  Pro Leu Tyr Gln Ser  Leu Leu Lys Lys Arg  Leu Glu Glu 
    1115                 1120                 1125             


Asn Gly  Gly Asp Pro Lys Lys  Ala Phe Thr Gly Lys  Asn Ser Leu 
    1130                 1135                 1140             


Ala Lys  Asn Pro Ile Tyr Leu  Asp Asp Thr Gln Thr  Glu Gln Leu 
    1145                 1150                 1155             


Pro Glu  Lys Val Lys Leu Val  Trp Leu Glu Asn Ser  Phe Ser Ile 
    1160                 1165                 1170             


Arg Lys  Asp Ile Thr Pro Glu  Asn Phe Lys Asp Glu  Lys Leu Ile 
    1175                 1180                 1185             


Asp Lys  Ile Leu Asp Glu Gly  Val Lys Arg Ile Leu  Lys Glu Arg 
    1190                 1195                 1200             


Leu Gln  Ser Phe Gly Asn Asp  Ala Lys Lys Ala Phe  Ser Asp Leu 
    1205                 1210                 1215             


Asp Lys  Asn Pro Ile Trp Leu  Asn Lys Glu Lys Gly  Ile Thr Ile 
    1220                 1225                 1230             


Lys Arg  Val Thr Ile Ser Gly  Val Ala Asn Ala Glu  Pro Leu His 
    1235                 1240                 1245             


Thr Lys  Lys Asp His Leu Gly  Lys Glu Ile Leu Asp  Lys Glu Gly 
    1250                 1255                 1260             


Lys Pro  Ile Pro Val Asp Phe  Ile Ser Thr Gly Ser  Asn His His 
    1265                 1270                 1275             


Val Ala  Val Tyr Arg Asp Glu  Lys Gly Asn Leu Gln  Glu Lys Val 
    1280                 1285                 1290             


Val Ser  Val Phe Glu Ala Val  Val Arg Ala Asn Gln  Gly Leu Pro 
    1295                 1300                 1305             


Ile Ile  Asp Lys Thr Tyr Asn  Gln His Leu Gly Trp  Glu Phe Leu 
    1310                 1315                 1320             


Phe Thr  Met Lys Gln Asn Glu  Met Phe Val Phe Pro  Asn Glu Ser 
    1325                 1330                 1335             


Thr Gly  Phe Asn Pro Asn Asp  Ile Asp Leu Leu Asp  Pro Lys Asn 
    1340                 1345                 1350             


Lys Arg  Ile Ile Ser Pro Asn  Leu Phe Arg Val Gln  Lys Phe Ser 
    1355                 1360                 1365             


Lys Leu  Gln Tyr Gly Asn Ser  Phe Val Arg Glu Phe  Val Phe Arg 
    1370                 1375                 1380             


His His  Leu Glu Thr Thr Val  Glu Asp Arg Lys Glu  Leu Gln Gly 
    1385                 1390                 1395             


Ile Thr  Tyr Lys Gln Met Lys  Asn Ser Thr Phe Phe  Asn Asp Ile 
    1400                 1405                 1410             


Ile Lys  Val Arg Ile Asn His  Leu Gly Asp Ile Val  Ser Ile Gly 
    1415                 1420                 1425             


Glu Tyr  
    1430 


<210>  1233
<211>  1430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1233

Met Lys His Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Lys Glu Ala Gln Asn Asp Asn Glu Gln Ser Glu Ile Val 
            20                  25                  30          


Asp Val Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Pro Ile Ser Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Lys Glu 
65                  70                  75                  80  


Leu Ile Glu Leu Leu Lys Glu His Gln Leu Ile Ser Thr Asp Thr Ile 
                85                  90                  95      


Leu Thr Glu Asn Gly Lys Gly Thr Thr Phe Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Lys Glu Lys Ile Ser Leu Glu Glu Leu Ala Arg Val 
        115                 120                 125             


Phe Leu Thr Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Glu Asp Glu Gly Gln Leu Ile Asp Gly Met Glu Ile Ala Lys 
145                 150                 155                 160 


Arg Leu Tyr Glu Glu Asn Leu Thr Thr Gly Gln Leu Ser Leu Gln Leu 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Ser Leu Pro Asp Tyr Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Phe Glu Leu Asp Lys Val Trp Asn Phe Gln Lys Gln Phe His Ser 
        195                 200                 205             


His Ile Leu Thr Asp Glu Phe Tyr Ala Gln Leu Lys Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ser Ala Leu Phe Leu Ala Lys Tyr Gln Ile Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Ser Arg Glu Asp Lys Lys Leu Gln Ala Tyr Gln Trp 
                245                 250                 255     


Arg Val Asp Ala Leu Ser Lys Glu Leu Thr Arg Glu Gln Leu Ala Phe 
            260                 265                 270         


Val Ile Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Glu Thr 
    290                 295                 300                 


Val Gly Glu Tyr Leu Trp Lys Gln Ile Ala Ala Asn Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Lys Thr Gln Ser Ala Phe Tyr Pro Glu Ile Leu Thr Asp 
            340                 345                 350         


Glu Leu Lys Lys Glu Leu Arg Asp Val Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Ser Leu Val Ser Phe Cys Glu Phe Glu Asn Lys 
    370                 375                 380                 


Glu Ile Glu Ile Lys Lys Glu Asp Gly Thr Ser Thr Lys Lys Val Ile 
385                 390                 395                 400 


Gly Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys 
                405                 410                 415     


Ile Trp Gln Asn Leu Asn Asn Val Leu Ile Arg Lys Lys Gly Ser Lys 
            420                 425                 430         


Thr Lys Lys Val Asp Lys Asn Gln Gln Ala Val Leu Phe Glu Glu Glu 
        435                 440                 445             


Lys Thr Ile Phe Glu Leu Asp Leu Glu Ala Lys Gln Ala Leu Phe Asn 
    450                 455                 460                 


Glu Leu Asn Leu Lys Gly Asn Leu Lys Ala Tyr Tyr Cys Leu Glu Ile 
465                 470                 475                 480 


Leu Gly Tyr Ser Pro Lys Glu Trp Glu Met Asn Tyr Ser Val Leu Glu 
                485                 490                 495     


Gly Asn Arg Thr Asn Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Thr 
            500                 505                 510         


Asp Leu Glu Gly Tyr Asp Ala Lys Asn His Leu Lys Val Lys Leu Asn 
        515                 520                 525             


Lys Asp Glu Val Glu Leu Asp Asp Leu Lys Val Pro Ala Ser Glu Ile 
    530                 535                 540                 


Lys Asp Met Val Gln Ala Ile Phe Lys Glu Ala Gly Ile Asn Thr Asp 
545                 550                 555                 560 


Ile Leu His Phe Asn Ala Glu Leu Asp Gly Lys Asp Phe Glu Asn Gln 
                565                 570                 575     


Ala Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp 
            580                 585                 590         


Ser Lys Ser Gly Asn Glu Leu Leu Tyr Lys Leu Leu Glu Thr Lys Phe 
        595                 600                 605             


Gly Phe Lys Lys Glu Tyr Ala Gln Ile Leu Ala Asn Val Ser Leu Ser 
    610                 615                 620                 


Asp Asp Tyr Gly Ser Leu Ser Thr Lys Ala Met Arg Lys Ile His Pro 
625                 630                 635                 640 


Tyr Ile Lys Glu Asn Lys Phe Ser Thr Ala Cys Glu Leu Ala Gly Tyr 
                645                 650                 655     


Arg His Ser Lys His Ser Leu Thr Lys Glu Glu Asn Asp Asn Arg Val 
            660                 665                 670         


Leu Lys Asp Ser Leu Glu Val Leu Lys Lys Asn Ser Leu Arg Gln Pro 
        675                 680                 685             


Val Val Glu Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Ala Leu 
    690                 695                 700                 


Leu Lys Lys Tyr Arg Thr Glu Asn Pro Asn Phe Lys Phe Asp Glu Ile 
705                 710                 715                 720 


Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Glu Glu Arg Ala 
                725                 730                 735     


Asn Met Thr Val Asn Ile Asn Lys Ala Lys Thr Glu His Glu Lys Ile 
            740                 745                 750         


Ile Lys Ile Leu Lys Asn Glu Phe Gly Leu Pro Asn Pro Thr Arg Asn 
        755                 760                 765             


Asp Ile Ile Arg Tyr Arg Leu Tyr Glu Glu Leu Lys Ala Asn Gly Tyr 
    770                 775                 780                 


Lys Asp Leu Tyr Thr Asn Glu Tyr Ile Glu Lys Gln Asp Leu Phe Thr 
785                 790                 795                 800 


Lys Asn Ile Asp Ile Glu His Ile Ile Pro Lys Ser Arg Val Phe Asp 
                805                 810                 815     


Asp Ser Phe Ser Asn Lys Thr Leu Ser Phe Arg Lys Thr Asn Leu Asp 
            820                 825                 830         


Lys Gly Glu Arg Thr Ala Phe Asp Tyr Ile Glu Ser Lys Phe Gly Ala 
        835                 840                 845             


Asp Lys Leu Glu Glu Tyr Val Ala Arg Val Glu Asp Leu Tyr Lys Asn 
    850                 855                 860                 


Asn Ile Ile Ser Lys Ala Lys Tyr Gln Lys Leu Leu Lys Lys Glu Ser 
865                 870                 875                 880 


Glu Ile Gly Asp Gly Phe Val Glu Arg Asp Leu Arg Glu Thr Gln Tyr 
                885                 890                 895     


Ile Ala Lys Lys Ala Lys Glu Ile Leu Leu Gln Ile Thr Pro Asn Val 
            900                 905                 910         


Leu Ser Thr Ser Gly Gly Ile Thr Asp Arg Leu Arg Glu Asp Trp Asp 
        915                 920                 925             


Leu Ile Asn Val Met Lys Glu Leu Asn Leu Pro Lys Tyr Arg Ala Leu 
    930                 935                 940                 


Gly Leu Thr Gln Met Glu Asp Arg Lys Tyr Gly Gln Gln Val Glu Val 
945                 950                 955                 960 


Ile Ile Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp 
                965                 970                 975     


Ala Leu Thr Val Ala Phe Thr Lys His Asn His Ile Gln Tyr Leu Asn 
            980                 985                 990         


Tyr Leu Asn Ala Arg Lys Asn Glu  Lys His Arg Glu His  Ser Asn Ile 
        995                 1000                 1005             


Met Gly  Ile Gln Gln Leu Glu  Thr Ile Lys Val Thr  Asp Lys Asn 
    1010                 1015                 1020             


Gly Asn  Glu Lys Arg Val Phe  Lys Ala Pro Met Pro  Asn Phe Arg 
    1025                 1030                 1035             


Gln Val  Thr Lys Ala Phe Leu  Glu Asn Val Leu Ile  Ser His Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Val Thr  Lys Asn Lys Asn Lys  Val Ala Gly 
    1055                 1060                 1065             


Ser Asn  Lys Val Gln Glu Val  Leu Thr Pro Arg Gly  Gln Leu His 
    1070                 1075                 1080             


Lys Glu  Thr Val Tyr Gly Lys  Tyr Gln Tyr Tyr Val  Gln Lys Glu 
    1085                 1090                 1095             


Glu Lys  Ile Gly Gly Lys Phe  Asp Leu Glu Thr Ile  Lys Lys Val 
    1100                 1105                 1110             


Ala Asn  Pro Leu Tyr Gln Ser  Leu Leu Lys Lys Arg  Leu Glu Glu 
    1115                 1120                 1125             


Asn Gly  Gly Asp Pro Lys Lys  Ala Phe Thr Gly Lys  Asn Ser Leu 
    1130                 1135                 1140             


Ala Lys  Asn Pro Ile Tyr Leu  Asp Asp Ala Gln Thr  Glu Gln Leu 
    1145                 1150                 1155             


Pro Glu  Lys Val Lys Leu Val  Trp Leu Glu Asn Ser  Phe Ser Ile 
    1160                 1165                 1170             


Arg Lys  Asp Ile Thr Pro Glu  Asn Phe Lys Asp Glu  Lys Leu Ile 
    1175                 1180                 1185             


Asp Lys  Ile Leu Asp Glu Gly  Val Lys Arg Ile Leu  Lys Glu Arg 
    1190                 1195                 1200             


Leu Gln  Ser Phe Gly Asn Asp  Ala Lys Lys Ala Phe  Ser Asp Leu 
    1205                 1210                 1215             


Asp Lys  Asn Pro Ile Trp Leu  Asn Lys Glu Lys Gly  Ile Ala Ile 
    1220                 1225                 1230             


Lys Arg  Val Ala Ile Ser Gly  Val Ala Asn Ala Glu  Pro Leu His 
    1235                 1240                 1245             


Thr Lys  Lys Asp His Leu Gly  Lys Glu Ile Leu Asp  Lys Glu Gly 
    1250                 1255                 1260             


Lys Ser  Ile Pro Val Asp Phe  Ile Ser Thr Gly Asn  Asn His His 
    1265                 1270                 1275             


Val Ala  Val Tyr Arg Asp Glu  Lys Gly Asn Leu Gln  Glu Lys Val 
    1280                 1285                 1290             


Val Ser  Val Phe Glu Ala Val  Val Arg Ala Asn Gln  Gly Leu Pro 
    1295                 1300                 1305             


Ile Ile  Asp Lys Thr Tyr Asn  Gln His Leu Gly Trp  Glu Phe Leu 
    1310                 1315                 1320             


Phe Thr  Met Lys Gln Asn Glu  Met Phe Val Phe Ser  Asn Glu Ser 
    1325                 1330                 1335             


Thr Gly  Phe Asn Pro Asn Asp  Ile Asp Leu Leu Asp  Pro Lys Asn 
    1340                 1345                 1350             


Lys Lys  Ile Ile Ser Pro Asn  Leu Phe Arg Val Gln  Lys Phe Ser 
    1355                 1360                 1365             


Lys Leu  Gln Tyr Gly Asn Ser  Phe Val Arg Glu Phe  Val Phe Arg 
    1370                 1375                 1380             


His His  Leu Glu Thr Thr Val  Glu Asp Arg Lys Glu  Leu Gln Gly 
    1385                 1390                 1395             


Ile Thr  Tyr Lys Gln Met Lys  Asn Ser Ala Phe Phe  Asn Asp Ile 
    1400                 1405                 1410             


Ile Lys  Val Arg Ile Asn His  Leu Gly Gly Ile Val  Ser Val Gly 
    1415                 1420                 1425             


Glu Tyr  
    1430 


<210>  1234
<211>  1430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1234

Met Lys Asp Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Lys Glu Ala Gln Asn Asp Asn Glu Thr Ser Glu Ile Val 
            20                  25                  30          


Asp Leu Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Pro Ile Ser Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Lys Glu 
65                  70                  75                  80  


Leu Ile Glu Leu Leu Lys Glu His Gln Leu Ile Ser Thr Asp Thr Ile 
                85                  90                  95      


Leu Thr Glu Asn Gly Lys Gly Thr Ser Phe Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Lys Glu Lys Ile Ser Leu Glu Glu Leu Ala Arg Val 
        115                 120                 125             


Phe Leu Thr Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Glu Asp Glu Gly Gln Leu Ile Asp Gly Met Glu Ile Ala Lys 
145                 150                 155                 160 


Arg Leu Tyr Glu Glu Asn Leu Thr Thr Gly Gln Leu Ser Leu Gln Leu 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Ser Leu Pro Asp Tyr Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Phe Glu Leu Asp Lys Val Trp Asn Phe Gln Lys Gln Phe His Ser 
        195                 200                 205             


His Ile Leu Thr Asp Glu Phe Tyr Ala Gln Leu Lys Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ser Ala Leu Phe Leu Ala Lys Tyr Gln Ile Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Ser Arg Glu Asp Lys Lys Leu Gln Ala Tyr Gln Trp 
                245                 250                 255     


Arg Val Asp Ala Leu Ser Lys Glu Leu Thr Arg Glu Gln Leu Ala Phe 
            260                 265                 270         


Val Ile Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Glu Thr 
    290                 295                 300                 


Val Gly Glu Tyr Leu Trp Lys Gln Ile Ala Ala Asn Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Asn Thr Gln Ala Gln Phe Tyr Pro Gln Ile Leu Thr Asp 
            340                 345                 350         


Glu Leu Lys Lys Glu Leu Arg Asp Val Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Ser Leu Val Ser Phe Cys Glu Phe Glu Asn Lys 
    370                 375                 380                 


Glu Ile Glu Ile Lys Lys Glu Asp Gly Thr Ser Thr Lys Lys Val Ile 
385                 390                 395                 400 


Gly Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys 
                405                 410                 415     


Ile Trp Gln Asn Leu Asn Asn Val Leu Ile Arg Lys Lys Gly Ser Lys 
            420                 425                 430         


Thr Lys Lys Ala Ala Lys Asn Gln Gln Ala Val Leu Phe Glu Glu Glu 
        435                 440                 445             


Lys Thr Ile Ser Glu Leu Asp Leu Glu Ala Lys Gln Ala Leu Phe Asn 
    450                 455                 460                 


Glu Leu Asn Leu Lys Gly Asn Leu Lys Ala Asp Tyr Cys Leu Glu Ile 
465                 470                 475                 480 


Leu Gly Tyr Ser Pro Lys Glu Trp Glu Met Asn Tyr Ser Val Leu Glu 
                485                 490                 495     


Gly Asn Arg Thr Asn Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Thr 
            500                 505                 510         


Asp Leu Glu Gly Tyr Asp Ala Lys Asn His Leu Lys Val Lys Leu Asn 
        515                 520                 525             


Lys Asp Glu Val Glu Leu Asp Asp Leu Lys Val Pro Ala Ser Glu Ile 
    530                 535                 540                 


Lys Asp Met Val Gln Ala Ile Phe Lys Glu Ala Gly Ile Asn Thr Asp 
545                 550                 555                 560 


Ile Leu His Phe Asn Ala Glu Leu Asp Gly Lys Asp Phe Glu Asn Gln 
                565                 570                 575     


Ala Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp 
            580                 585                 590         


Ser Lys Ser Gly Asn Glu Leu Leu Tyr Lys Leu Leu Glu Thr Lys Phe 
        595                 600                 605             


Gly Phe Lys Lys Glu Tyr Ala Gln Ile Leu Ala Asn Val Ser Leu Ser 
    610                 615                 620                 


Asp Asp Tyr Gly Ser Leu Ser Thr Lys Ala Met Arg Lys Ile His Pro 
625                 630                 635                 640 


Tyr Ile Lys Glu Asn Lys Phe Ser Thr Ala Cys Glu Leu Ala Gly Tyr 
                645                 650                 655     


Arg His Ser Lys His Ser Leu Thr Lys Glu Glu Asn Asp Asn Arg Val 
            660                 665                 670         


Leu Lys Asp Ser Leu Glu Val Leu Lys Lys Asn Ser Leu Arg Gln Pro 
        675                 680                 685             


Val Val Glu Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Ala Leu 
    690                 695                 700                 


Leu Lys Lys Tyr Arg Thr Glu Asn Pro Asn Phe Lys Phe Asp Glu Ile 
705                 710                 715                 720 


Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Glu Glu Arg Ala 
                725                 730                 735     


Asn Met Thr Ala Asn Ile Asn Lys Ala Lys Thr Glu His Glu Lys Ile 
            740                 745                 750         


Ile Lys Ile Leu Lys Asn Glu Phe Gly Leu Pro Asn Pro Thr Arg Asn 
        755                 760                 765             


Asp Ile Ile Arg Tyr Arg Leu Tyr Glu Glu Leu Lys Ala Asn Gly Tyr 
    770                 775                 780                 


Lys Asp Leu Tyr Thr Asn Ile Lys Ile Pro Gln Glu Lys Leu Phe Thr 
785                 790                 795                 800 


Lys Glu Ile Asp Ile Glu His Ile Ile Pro Lys Ser Arg Val Phe Asp 
                805                 810                 815     


Asp Ser Phe Ser Asn Lys Thr Leu Ser Phe Arg Lys Thr Asn Leu Asp 
            820                 825                 830         


Lys Gly Glu Arg Thr Ala Phe Asp Tyr Met Glu Ser Lys Phe Gly Ala 
        835                 840                 845             


Asp Lys Leu Glu Glu Tyr Val Ala Arg Val Glu Asp Leu Tyr Lys Asn 
    850                 855                 860                 


Asn Ile Ile Ser Lys Ala Lys Tyr Gln Lys Leu Leu Lys Lys Glu Ser 
865                 870                 875                 880 


Glu Ile Gly Asp Gly Phe Val Glu Arg Asp Leu Arg Glu Thr Gln Tyr 
                885                 890                 895     


Ile Ala Lys Lys Ala Lys Glu Ile Leu Leu Gln Ile Thr Pro Asn Val 
            900                 905                 910         


Leu Ser Thr Ser Gly Gly Ile Thr Asp Arg Leu Arg Glu Asp Trp Asp 
        915                 920                 925             


Leu Ile Asn Val Met Lys Glu Leu Asn Leu Pro Lys Tyr Arg Ala Leu 
    930                 935                 940                 


Gly Leu Thr Glu Met Glu Asp Arg Lys Tyr Gly Gln Gln Val Glu Val 
945                 950                 955                 960 


Ile Ile Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp 
                965                 970                 975     


Ala Leu Thr Val Ala Phe Thr Lys His Asn His Ile Gln Tyr Leu Asn 
            980                 985                 990         


Tyr Leu Asn Ala Arg Lys Asn Glu  Lys His Lys Glu His  Ser Asn Ile 
        995                 1000                 1005             


Met Gly  Ile Gln Gln Leu Glu  Thr Ile Lys Val Thr  Asp Lys Asn 
    1010                 1015                 1020             


Gly Asn  Glu Lys Arg Val Phe  Lys Ala Pro Met Pro  Asn Phe Arg 
    1025                 1030                 1035             


Gln Val  Thr Lys Ala Phe Leu  Glu Asn Ile Leu Ile  Ser His Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Val Thr  Lys Asn Lys Asn Lys  Val Ala Gly 
    1055                 1060                 1065             


Ser Asn  Lys Val Gln Glu Val  Leu Thr Pro Arg Gly  Gln Leu His 
    1070                 1075                 1080             


Lys Glu  Thr Val Tyr Gly Lys  Tyr Gln Tyr Tyr Val  Gln Lys Glu 
    1085                 1090                 1095             


Glu Lys  Ile Gly Gly Lys Phe  Asp Leu Glu Thr Ile  Lys Lys Val 
    1100                 1105                 1110             


Ala Asn  Pro Leu Tyr Gln Ser  Leu Leu Lys Lys Arg  Leu Glu Glu 
    1115                 1120                 1125             


Asn Gly  Gly Asp Pro Lys Lys  Ala Phe Thr Gly Lys  Tyr Ser Leu 
    1130                 1135                 1140             


Ala Lys  Asn Pro Ile Tyr Leu  Asp Asp Ala Gln Thr  Glu Gln Leu 
    1145                 1150                 1155             


Pro Glu  Lys Val Lys Leu Val  Trp Leu Glu Asn Ser  Phe Ser Ile 
    1160                 1165                 1170             


Arg Lys  Asp Ile Thr Pro Glu  Asn Phe Lys Asp Glu  Lys Leu Ile 
    1175                 1180                 1185             


Asp Lys  Ile Leu Asp Glu Gly  Val Lys Arg Ile Leu  Lys Glu Arg 
    1190                 1195                 1200             


Leu Gln  Ser Phe Gly Asn Asp  Ala Lys Lys Ala Phe  Ser Asp Leu 
    1205                 1210                 1215             


Asp Lys  Asn Pro Ile Trp Leu  Asn Lys Glu Lys Gly  Ile Thr Ile 
    1220                 1225                 1230             


Lys Arg  Val Thr Ile Ser Gly  Val Ala Asn Ala Glu  Pro Leu His 
    1235                 1240                 1245             


Thr Lys  Lys Asp His Leu Gly  Lys Glu Ile Leu Asp  Lys Glu Gly 
    1250                 1255                 1260             


Lys Pro  Ile Pro Val Asp Phe  Ile Ser Thr Gly Ser  Asn His His 
    1265                 1270                 1275             


Val Ala  Val Tyr Arg Asp Glu  Lys Gly Asn Leu Gln  Glu Lys Val 
    1280                 1285                 1290             


Val Ser  Val Phe Glu Ala Val  Val Arg Ala Asn Gln  Gly Leu Pro 
    1295                 1300                 1305             


Ile Ile  Asp Lys Thr Tyr Asn  Gln His Leu Gly Trp  Glu Phe Leu 
    1310                 1315                 1320             


Phe Thr  Met Lys Gln Asn Glu  Met Phe Val Phe Pro  Asn Glu Ser 
    1325                 1330                 1335             


Thr Gly  Phe Asn Pro Asn Asp  Ile Asp Leu Leu Asp  Pro Lys Asn 
    1340                 1345                 1350             


Lys Arg  Ile Ile Ser Pro Asn  Leu Phe Arg Val Gln  Thr Ile Ser 
    1355                 1360                 1365             


Val Val  Lys Tyr Gly Asn Ala  Ile Val Arg Asp Phe  Lys Phe Arg 
    1370                 1375                 1380             


His His  Leu Glu Thr Thr Val  Glu Asp Lys Lys Glu  Leu Gln Gly 
    1385                 1390                 1395             


Lys Thr  Tyr Gln Gln Ile Lys  Ser Leu Ser Pro Leu  Asn Asp Ile 
    1400                 1405                 1410             


Ile Lys  Val Arg Ile Asn His  Leu Gly Asp Ile Val  Ser Val Gly 
    1415                 1420                 1425             


Glu Tyr  
    1430 


<210>  1235
<211>  1430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1235

Met Lys His Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Tyr Val Lys Glu Ala Glu Asn Asp Thr Glu Thr Ser Glu Ile Val 
            20                  25                  30          


Asp Leu Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Pro Ile Ser Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Lys Glu 
65                  70                  75                  80  


Leu Ile Glu Leu Leu Lys Glu His Gln Leu Ile Ser Glu Asn Thr Ile 
                85                  90                  95      


Leu Thr Glu Asn Gly Lys Gly Thr Thr Phe Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Lys Glu Lys Ile Ser Leu Gly Glu Leu Ala Arg Val 
        115                 120                 125             


Phe Leu Thr Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Glu Asp Glu Gly Gln Leu Ile Asp Gly Met Glu Ile Ala Lys 
145                 150                 155                 160 


Arg Leu Tyr Glu Glu Asn Leu Thr Thr Gly Gln Leu Ser Leu Gln Leu 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Ser Leu Pro Asp Tyr Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Phe Glu Leu Asp Lys Val Trp Asn Phe Gln Lys Gln Phe His Ser 
        195                 200                 205             


Asp Ile Leu Thr Asp Glu Phe Tyr Ala Gln Leu Lys Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ser Ala Leu Phe Leu Ala Lys Tyr Gln Ile Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Ser Arg Glu Asp Lys Lys Leu Gln Ala Tyr Gln Trp 
                245                 250                 255     


Arg Val Asp Ala Leu Ser Lys Glu Leu Thr Arg Glu Gln Leu Ala Phe 
            260                 265                 270         


Val Ile Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Glu Thr 
    290                 295                 300                 


Val Gly Glu Tyr Leu Trp Lys Gln Ile Ala Ala Asn Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Asn Thr Gln Ala Gln Phe Tyr Pro Gln Ile Leu Thr Asp 
            340                 345                 350         


Glu Leu Lys Lys Glu Leu Arg Asp Val Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Ser Leu Val Ser Phe Cys Glu Phe Glu Asn Lys 
    370                 375                 380                 


Glu Ile Glu Ile Lys Lys Glu Asp Gly Thr Ser Thr Lys Lys Val Ile 
385                 390                 395                 400 


Gly Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys 
                405                 410                 415     


Ile Trp Gln Asn Leu Asn Asn Val Leu Ile Arg Lys Lys Gly Ser Lys 
            420                 425                 430         


Thr Lys Lys Ala Ala Lys Asn Gln Gln Ala Val Leu Phe Glu Glu Glu 
        435                 440                 445             


Lys Thr Ile Ser Glu Leu Asp Leu Glu Ala Lys Gln Ala Leu Phe Asn 
    450                 455                 460                 


Glu Leu Asn Leu Lys Gly Asn Leu Lys Ala Asp Tyr Cys Leu Glu Ile 
465                 470                 475                 480 


Leu Gly Tyr Ser Pro Lys Glu Trp Glu Met Asn Tyr Ser Val Leu Glu 
                485                 490                 495     


Gly Asn Arg Thr Asn Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Thr 
            500                 505                 510         


Asp Leu Glu Gly Tyr Asp Ala Lys Asn His Leu Lys Val Lys Leu Asn 
        515                 520                 525             


Lys Asp Glu Val Glu Leu Asp Asp Leu Lys Val Pro Ala Ser Glu Ile 
    530                 535                 540                 


Lys Glu Met Val Gln Ala Ile Phe Lys Glu Ala Gly Ile Asn Thr Asp 
545                 550                 555                 560 


Ile Leu His Phe Asn Ala Glu Leu Asp Gly Lys Asp Phe Glu Asn Gln 
                565                 570                 575     


Ala Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp 
            580                 585                 590         


Ser Lys Ser Gly Asn Glu Leu Leu Tyr Lys Leu Leu Glu Thr Lys Phe 
        595                 600                 605             


Gly Phe Lys Lys Glu Tyr Ala Gln Ile Leu Ala Asn Val Ser Leu Ser 
    610                 615                 620                 


Asp Asp Tyr Gly Asn Leu Ser Thr Lys Ala Met Arg Lys Ile Tyr Pro 
625                 630                 635                 640 


Tyr Ile Lys Glu Asn Lys Phe Ser Thr Ala Cys Glu Leu Ala Gly Tyr 
                645                 650                 655     


Arg His Ser Lys His Ser Leu Thr Lys Glu Glu Asn Asp Asn Arg Val 
            660                 665                 670         


Leu Lys Asp Ser Leu Glu Val Leu Lys Lys Asn Ser Leu Arg Gln Pro 
        675                 680                 685             


Val Val Glu Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn Ala Leu 
    690                 695                 700                 


Leu Lys Lys Tyr Arg Thr Glu Asn Pro Asn Phe Lys Phe Asp Glu Ile 
705                 710                 715                 720 


Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Glu Glu Arg Ala 
                725                 730                 735     


Asn Met Thr Ala Asn Ile Asn Lys Ala Lys Thr Glu His Glu Lys Ile 
            740                 745                 750         


Ile Lys Ile Leu Lys Asn Glu Phe Gly Leu Pro Asn Pro Thr Arg Asn 
        755                 760                 765             


Asp Ile Ile Arg Tyr Arg Leu Tyr Glu Glu Leu Lys Ala Asn Gly Tyr 
    770                 775                 780                 


Lys Asp Leu Tyr Thr Asn Ile Lys Ile Pro Gln Glu Lys Leu Phe Thr 
785                 790                 795                 800 


Lys Glu Ile Asp Ile Glu His Ile Ile Pro Lys Ser Arg Val Phe Asp 
                805                 810                 815     


Asp Ser Phe Ser Asn Lys Thr Leu Ser Phe Arg Lys Thr Asn Leu Asp 
            820                 825                 830         


Lys Gly Glu Arg Thr Ala Phe Asp Tyr Met Glu Ser Lys Phe Gly Ala 
        835                 840                 845             


Asp Lys Leu Glu Glu Tyr Val Ala Arg Val Glu Asp Leu Tyr Lys Asn 
    850                 855                 860                 


Asn Ile Ile Ser Lys Ala Lys Tyr Gln Lys Leu Leu Lys Lys Glu Ser 
865                 870                 875                 880 


Glu Ile Gly Asp Gly Phe Val Glu Arg Asp Leu Arg Glu Thr Gln Tyr 
                885                 890                 895     


Ile Ala Lys Lys Ala Lys Glu Ile Leu Leu Gln Ile Thr Arg Asn Val 
            900                 905                 910         


Leu Ser Thr Ser Gly Gly Ile Thr Asp Arg Leu Arg Glu Asp Trp Asp 
        915                 920                 925             


Leu Val Asn Val Met Lys Glu Leu Asn Leu Pro Lys Tyr Arg Ala Leu 
    930                 935                 940                 


Gly Leu Thr Glu Met Glu Asp Arg Lys Tyr Gly Gln Gln Val Glu Val 
945                 950                 955                 960 


Ile Ile Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala Met Asp 
                965                 970                 975     


Ala Leu Thr Val Ala Phe Thr Lys His Asn His Ile Gln Tyr Leu Asn 
            980                 985                 990         


Tyr Leu Asn Ala Arg Lys Asn Glu  Lys His Arg Glu His  Ser Asn Ile 
        995                 1000                 1005             


Met Gly  Ile Gln Gln Leu Glu  Thr Ile Lys Val Thr  Asp Lys Asn 
    1010                 1015                 1020             


Gly Asn  Glu Lys Arg Val Phe  Lys Ala Pro Met Pro  Asn Phe Arg 
    1025                 1030                 1035             


Gln Val  Thr Lys Ala Phe Leu  Glu Asn Val Leu Ile  Ser His Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Val Thr  Lys Asn Lys Asn Lys  Val Ala Gly 
    1055                 1060                 1065             


Ser Asn  Lys Val Gln Glu Val  Leu Thr Pro Arg Gly  Gln Leu His 
    1070                 1075                 1080             


Lys Glu  Thr Val Tyr Gly Lys  Tyr Gln Tyr Tyr Val  Gln Lys Glu 
    1085                 1090                 1095             


Glu Lys  Ile Gly Gly Lys Phe  Asp Leu Glu Thr Ile  Lys Lys Val 
    1100                 1105                 1110             


Ala Asn  Pro Leu Tyr Gln Ser  Leu Leu Lys Lys Arg  Leu Glu Glu 
    1115                 1120                 1125             


Asn Gly  Gly Asp Pro Lys Lys  Ala Phe Thr Gly Lys  Asn Ser Leu 
    1130                 1135                 1140             


Ala Lys  Asn Pro Ile Tyr Leu  Asp Asp Ala Gln Thr  Glu Gln Leu 
    1145                 1150                 1155             


Pro Glu  Lys Val Lys Leu Val  Trp Leu Glu Asn Ser  Phe Ser Ile 
    1160                 1165                 1170             


Arg Lys  Asp Ile Thr Pro Glu  Asn Phe Lys Asp Glu  Lys Leu Ile 
    1175                 1180                 1185             


Asp Lys  Ile Leu Asp Glu Gly  Val Lys Arg Ile Leu  Lys Glu Arg 
    1190                 1195                 1200             


Leu Gln  Ser Phe Gly Asn Asp  Ala Lys Lys Ala Phe  Ser Asp Leu 
    1205                 1210                 1215             


Asp Lys  Asn Pro Ile Trp Leu  Asn Lys Glu Lys Gly  Ile Thr Ile 
    1220                 1225                 1230             


Lys Arg  Val Thr Ile Ser Gly  Val Ala Asn Ala Glu  Pro Leu His 
    1235                 1240                 1245             


Thr Lys  Lys Asp His Leu Gly  Lys Glu Ile Leu Asp  Lys Glu Gly 
    1250                 1255                 1260             


Lys Pro  Ile Pro Val Asp Phe  Ile Ser Thr Gly Ser  Asn His His 
    1265                 1270                 1275             


Val Ala  Val Tyr Arg Asp Glu  Lys Gly Asn Leu Gln  Glu Lys Val 
    1280                 1285                 1290             


Val Ser  Val Phe Glu Ala Val  Val Arg Ala Asn Gln  Gly Leu Pro 
    1295                 1300                 1305             


Ile Ile  Asp Lys Thr Tyr Asn  Glu His Leu Gly Trp  Glu Phe Leu 
    1310                 1315                 1320             


Phe Thr  Met Lys Gln Asn Glu  Met Phe Val Phe Pro  Asn Glu Ser 
    1325                 1330                 1335             


Thr Gly  Phe Asn Pro Asn Asp  Ile Asp Leu Leu Asp  Pro Lys Asn 
    1340                 1345                 1350             


Lys Arg  Ile Ile Ser Pro Asn  Leu Phe Arg Val Gln  Thr Ile Ser 
    1355                 1360                 1365             


Val Val  Lys Tyr Gly Asn Ala  Ile Val Arg Asp Phe  Lys Phe Arg 
    1370                 1375                 1380             


His His  Leu Glu Thr Thr Val  Glu Asp Lys Lys Glu  Leu Gln Gly 
    1385                 1390                 1395             


Lys Thr  Tyr Gln Gln Ile Lys  Ser Leu Ser Pro Leu  Asn Asp Ile 
    1400                 1405                 1410             


Ile Lys  Val Arg Ile Asn His  Leu Gly Asp Ile Val  Ser Val Gly 
    1415                 1420                 1425             


Glu Tyr  
    1430 


<210>  1236
<211>  1430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1236

Met Ala Gln Lys Tyr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Trp Ala Val Val Lys Glu Ala Glu Asn Asp Asn Glu Gln Ser Glu 
            20                  25                  30          


Ile Val Asp Val Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Lys 
        35                  40                  45              


Thr Asn Phe Glu Lys Gly Lys Pro Ile Ser Thr Asn Ala Asp Arg Thr 
    50                  55                  60                  


Leu Lys Arg Gly Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg 
65                  70                  75                  80  


Lys Glu Leu Ile Lys Ile Leu Lys Glu His Gln Leu Ile Ser Asp Asp 
                85                  90                  95      


Thr Ile Leu Ala Glu Asn Gly Lys Gly Thr Thr Tyr Gln Thr Leu Lys 
            100                 105                 110         


Leu Arg Ala Lys Ser Ser Lys Glu Lys Ile Ser Leu Glu Glu Leu Ala 
        115                 120                 125             


Arg Val Phe Leu Thr Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg 
    130                 135                 140                 


Lys Thr Lys Asn Glu Asp Glu Gly Gln Leu Ile Asp Gly Met Glu Ile 
145                 150                 155                 160 


Ala Lys Arg Leu Tyr Glu Glu Asn Leu Thr Thr Gly Gln Leu Ser Leu 
                165                 170                 175     


Gln Leu Leu Lys Glu Gly Lys Lys Ser Leu Pro Asp Tyr Tyr Arg Ser 
            180                 185                 190         


Asp Leu Glu Leu Glu Leu Asp Lys Val Trp Asn Phe Gln Lys Gln Phe 
        195                 200                 205             


His Ser Asp Ile Leu Thr Asp Glu Phe Tyr Thr Gln Leu Lys Gly Lys 
    210                 215                 220                 


Gly Gln Arg Ala Thr Ser Ala Leu Phe Leu Glu Lys Tyr Gln Ile Tyr 
225                 230                 235                 240 


Thr Val Glu Asn Lys Gly Asn Arg Glu Glu Lys Asn Leu Gln Ala Tyr 
                245                 250                 255     


Gln Trp Arg Val Asp Ala Leu Ser Lys Glu Leu Thr Arg Glu Glu Leu 
            260                 265                 270         


Ala Phe Val Ile Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly 
        275                 280                 285             


Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn 
    290                 295                 300                 


Glu Thr Val Gly Glu Tyr Leu Trp Lys Gln Ile Ala Ala Asn Pro His 
305                 310                 315                 320 


Thr Ser Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu 
                325                 330                 335     


Phe Glu Arg Ile Trp Asn Thr Gln Ala Gln Phe Tyr Pro Gln Ile Leu 
            340                 345                 350         


Thr Glu Ser Leu Lys Lys Glu Leu Arg Asp Val Val Ile Phe Tyr Gln 
        355                 360                 365             


Arg Lys Leu Lys Ser Gln Lys Ser Leu Val Ser Phe Cys Glu Phe Glu 
    370                 375                 380                 


Asn Lys Glu Ile Glu Ile Lys Asn Glu Asp Gly Thr Thr Thr Lys Lys 
385                 390                 395                 400 


Val Ile Gly Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu 
                405                 410                 415     


Phe Lys Ile Trp Gln Asn Leu Asn Asn Val Leu Ile Arg Lys Lys Gly 
            420                 425                 430         


Ser Lys Thr Glu Lys Val Ala Lys Asn Gln Gln Val Val Leu Phe Glu 
        435                 440                 445             


Glu Glu Lys Thr Ile Ser Glu Leu Asp Leu Glu Ala Lys Gln Ala Leu 
    450                 455                 460                 


Phe Asn Glu Leu Asn Leu Lys Gly Asn Leu Lys Ala Asp Tyr Cys Leu 
465                 470                 475                 480 


Glu Ile Leu Gly Tyr Ser Ser Lys Glu Trp Glu Met Asn Tyr Ser Val 
                485                 490                 495     


Leu Glu Gly Asn Arg Thr Asn Lys Ala Leu Tyr Glu Ala Tyr Leu Lys 
            500                 505                 510         


Ile Thr Asp Leu Glu Gly Tyr Asp Ala Lys Asn Tyr Leu Lys Val Lys 
        515                 520                 525             


Ser Asn Lys Asp Glu Val Glu Leu Asp Asp Leu Lys Val Pro Ala Ser 
    530                 535                 540                 


Glu Ile Lys Glu Met Val Gln Ala Ile Phe Lys Glu Ala Gly Ile Asn 
545                 550                 555                 560 


Thr Asp Ile Leu His Phe Asp Ala Glu Leu Asp Gly Lys Asp Phe Glu 
                565                 570                 575     


Asn Gln Gly Ser Tyr Lys Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly 
            580                 585                 590         


Asp Asp Ser Lys Ser Gly Asn Glu Leu Leu Tyr Lys Leu Leu Glu Thr 
        595                 600                 605             


Lys Phe Gly Leu Lys Lys Glu Tyr Ala Gln Ile Leu Ala Asn Ile Ser 
    610                 615                 620                 


Leu Ser Asp Asp Tyr Gly Asn Leu Ser Thr Lys Ala Met Arg Lys Ile 
625                 630                 635                 640 


His Pro Tyr Ile Lys Glu Asn Lys Phe Ser Thr Ala Cys Glu Leu Ala 
                645                 650                 655     


Gly Tyr Arg His Ser Lys His Ser Leu Thr Lys Glu Glu Asn Glu Lys 
            660                 665                 670         


Arg Val Leu Lys Asp Gly Leu Glu Val Leu Lys Lys Asn Ser Leu Arg 
        675                 680                 685             


Gln Pro Val Val Glu Lys Ile Leu Asn Gln Met Ile Asn Leu Val Asn 
    690                 695                 700                 


Ala Leu Leu Lys Lys Tyr Arg Thr Glu Asn Pro Asn Phe Lys Phe Asp 
705                 710                 715                 720 


Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Glu Glu 
                725                 730                 735     


Arg Ala Asn Met Thr Ala Asn Ile Asn Lys Ala Lys Thr Glu His Glu 
            740                 745                 750         


Lys Ile Asp Lys Ile Leu Lys Ser Glu Phe Gly Leu Pro Asn Pro Thr 
        755                 760                 765             


Arg Asn Asp Ile Ile Arg Tyr Arg Leu Tyr Glu Glu Leu Lys Ala Asn 
    770                 775                 780                 


Gly Tyr Lys Asp Leu Tyr Thr Asn Arg Tyr Ile Ser Ser Glu Lys Leu 
785                 790                 795                 800 


Phe Ser Lys Glu Ile Asp Ile Glu His Ile Ile Pro Lys Ser Arg Val 
                805                 810                 815     


Phe Asp Asp Ser Phe Ser Asn Lys Thr Ile Val Tyr Arg Thr Asp Asn 
            820                 825                 830         


Leu Asp Lys Gly Glu Gln Thr Ala Tyr Asp Tyr Ile Glu Ser Lys Phe 
        835                 840                 845             


Gly Ala Glu Lys Leu Lys Glu Tyr Met Thr Arg Val Lys Asp Leu Tyr 
    850                 855                 860                 


Gln Lys Lys Ile Ile Ser Lys Ala Lys Tyr Gln Lys Leu Leu Lys Lys 
865                 870                 875                 880 


Glu Ser Glu Ile Gly Asp Gly Phe Val Glu Arg Asp Leu Arg Glu Thr 
                885                 890                 895     


Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Leu Gln Ile Thr His 
            900                 905                 910         


Asn Val Leu Ser Thr Ser Gly Gly Ile Thr Asp Arg Leu Arg Lys Asp 
        915                 920                 925             


Trp Gly Leu Val Asn Val Met Lys Glu Leu Asn Leu Pro Lys Tyr Arg 
    930                 935                 940                 


Ala Leu Gly Leu Thr Lys Met Glu Asp Arg Lys Tyr Gly Gln Gln Val 
945                 950                 955                 960 


Glu Val Ile Thr Asp Trp Thr Lys Arg Asn Asp Leu Arg His His Ala 
                965                 970                 975     


Met Asp Ala Leu Thr Val Ala Phe Thr Lys His Asn His Ile Gln Tyr 
            980                 985                 990         


Leu Asn Tyr Leu Asn Ala Arg Lys  Asn Glu Lys His Lys  Glu His Asn 
        995                 1000                 1005             


Asn Ile  Met Ala Ile Gln Gln  Leu Glu Thr Ile Lys  Val Thr Asp 
    1010                 1015                 1020             


Glu Asn  Gly Asn Glu Lys Arg  Ile Phe Lys Ala Pro  Met Pro Asn 
    1025                 1030                 1035             


Phe Arg  Gln Val Ala Lys Thr  Phe Leu Glu Asn Val  Leu Ile Ser 
    1040                 1045                 1050             


His Lys  Ala Lys Asn Lys Val  Val Thr Lys Asn Lys  Asn Lys Ile 
    1055                 1060                 1065             


Ser Gly  Ser Asn Lys Val Gln  Glu Val Leu Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Lys Glu Thr Val Tyr  Gly Lys Tyr Gln Tyr  Tyr Val Gln 
    1085                 1090                 1095             


Lys Glu  Glu Lys Ile Gly Ala  Lys Phe Asp Ser Glu  Thr Ile Glu 
    1100                 1105                 1110             


Lys Val  Ala Asn Pro Leu Tyr  Gln Ser Leu Leu Lys  Lys Arg Leu 
    1115                 1120                 1125             


Glu Glu  Asn Gly Gly Asp Pro  Lys Lys Ala Phe Thr  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Ala Lys Asn Pro Ile  Tyr Leu Asp Asp Thr  Gln Thr Glu 
    1145                 1150                 1155             


Gln Leu  Pro Glu Lys Val Lys  Leu Val Trp Leu Glu  Asn Ser Phe 
    1160                 1165                 1170             


Ser Ile  Arg Lys Asp Ile Thr  Pro Glu Asn Phe Lys  Asp Glu Lys 
    1175                 1180                 1185             


Leu Ile  Asp Lys Ile Leu Asp  Glu Gly Val Lys Arg  Ile Leu Lys 
    1190                 1195                 1200             


Glu Arg  Leu Gln Ser Phe Gly  Asn Asp Ala Lys Lys  Ala Phe Ser 
    1205                 1210                 1215             


Asp Leu  Glu Lys Asn Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile 
    1220                 1225                 1230             


Ala Ile  Lys Arg Val Thr Ile  Ser Gly Val Ala Asn  Ala Glu Pro 
    1235                 1240                 1245             


Leu His  Thr Lys Lys Asp His  Leu Gly Lys Glu Ile  Leu Asp Lys 
    1250                 1255                 1260             


Glu Gly  Lys Pro Ile Pro Val  Asp Phe Ile Ser Thr  Gly Asn Asn 
    1265                 1270                 1275             


His His  Val Ala Val Tyr Arg  Asp Glu Lys Gly Asn  Leu Gln Glu 
    1280                 1285                 1290             


Lys Val  Leu Ser Val Phe Glu  Ala Val Val Arg Ala  Asn Gln Gly 
    1295                 1300                 1305             


Leu Pro  Ile Ile Glu Lys Thr  Tyr Asn Gln His Leu  Gly Trp Glu 
    1310                 1315                 1320             


Phe Leu  Phe Ser Met Lys Gln  Asn Glu Met Phe Val  Phe Pro Asn 
    1325                 1330                 1335             


Glu Ser  Thr Gly Phe Asn Pro  Asn Asp Ile Asp Leu  Leu Asp Pro 
    1340                 1345                 1350             


Lys Asn  Lys Arg Ile Ile Ser  Pro Asn Leu Phe Lys  Val Gln Lys 
    1355                 1360                 1365             


Leu Ala  Thr Lys Asn Tyr Met  Phe Thr His His Leu  Glu Thr Lys 
    1370                 1375                 1380             


Ser Ile  Asp Gly Asp Met Leu  Lys Asn Lys Lys Glu  Leu Ser Gly 
    1385                 1390                 1395             


Ile Leu  Tyr Asn Phe Ile Gln  Thr Pro Gln Lys Leu  Tyr Gly Ile 
    1400                 1405                 1410             


Ile Lys  Val Arg Ile Asn His  Leu Gly Asp Ile Val  Ser Val Gly 
    1415                 1420                 1425             


Glu Tyr  
    1430 


<210>  1237
<211>  1431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1237

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Ser Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Ser Lys Asn Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Ser Ile Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu His Arg Tyr Lys Leu Arg Arg Glu Ala 
65                  70                  75                  80  


Leu Ile Glu Val Leu Lys Glu Cys Lys Phe Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Val Lys Ala Leu Thr Glu Glu Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Ile Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Gly Gly Ala Glu Glu Asp Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Lys Leu Tyr Asp Ala Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Ser Ala Gly Lys Lys Val Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Gly Ile Leu Thr Ala Ala Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Asn Ala Phe Val Trp Lys Glu Thr 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Asn Glu Glu Ser Lys Asn Glu Gln Ile Glu Lys 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Lys Glu Asn Leu Gln Trp Arg Val Ser Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Ile Leu Gln Glu Ile Asn Thr Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Thr Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Tyr Leu Met Asp Ile Leu 
                325                 330                 335     


Asp Asn Asn Pro Asn Ala Ser Leu Arg Asn Ile Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Val Leu Trp Glu Lys Gln Ala Glu Phe 
        355                 360                 365             


His Lys Glu Leu Thr Gly Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Ser Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Lys Ile Asp Gly Lys Arg 
                405                 410                 415     


Lys Ile Arg Thr Val Gly Asn Arg Val Ile Pro Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Ala Val Gly Asn Lys Asn Lys Gln Arg Met Gln Asn Gly Ser Asn Glu 
    450                 455                 460                 


Pro Asp Asp Thr Glu Gln Leu Glu Leu Asn Gly Arg Arg Tyr Leu Tyr 
465                 470                 475                 480 


Gln Glu Glu Lys Glu Leu Leu Ala Lys Glu Leu Phe Val Arg Asp Lys 
                485                 490                 495     


Met Thr Lys Leu Asp Val Leu Arg Leu Leu Phe Asp Asn Pro Glu Glu 
            500                 505                 510         


Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn Lys Thr Gly Tyr Leu 
        515                 520                 525             


Phe Phe Gln Ala Tyr Ser Lys Met Ile Glu Met Ser Gly His Glu Pro 
    530                 535                 540                 


Val Asp Phe Lys Lys Pro Ile Glu Lys Ile Val Glu Gln Val Lys Ser 
545                 550                 555                 560 


Val Phe Val Leu Leu Asn Trp Asn Thr Asp Ile Leu Ser Phe Asp Ser 
                565                 570                 575     


Asn Lys Glu Leu Asp Lys Gln Ser Tyr Tyr Lys Leu Trp His Leu Leu 
            580                 585                 590         


Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly Asn Gly Cys Leu Val 
        595                 600                 605             


Gln Lys Ile Met Asp Ser Tyr Gly Leu Gly Lys Glu Phe Ala Lys Ile 
    610                 615                 620                 


Leu Ala Asn Ile Thr Phe Gln Glu Asp Tyr Gly Ser Leu Ser Ala Lys 
625                 630                 635                 640 


Ala Ile His Lys Ile Leu Pro His Leu Lys Glu Gly Asn Gln Tyr Asp 
                645                 650                 655     


Val Ala Cys Glu Tyr Ala Gly Tyr Arg His Ser Lys Ser Ser Leu Thr 
            660                 665                 670         


Lys Glu Glu Ile Ala Asn Lys Val Leu Lys Asp Lys Leu Glu Val Leu 
        675                 680                 685             


Pro Arg Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu Asn Gln 
    690                 695                 700                 


Met Val Asn Val Ile Asn Ala Ile Ile Glu Ala Tyr Gly Arg Pro Asp 
705                 710                 715                 720 


Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Lys Asn Ala Lys Glu 
                725                 730                 735     


Arg Glu Lys Leu Thr Lys Ser Ile Ala Glu Thr Thr Lys Thr His Glu 
            740                 745                 750         


Ala His Lys Lys Leu Leu Gln Glu Glu Phe Gly Leu Lys Asn Val Gly 
        755                 760                 765             


Arg Ser Asp Ile Leu Arg Tyr Lys Leu Tyr Lys Glu Leu Glu Ser Arg 
    770                 775                 780                 


Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile Pro Arg Glu Lys Leu 
785                 790                 795                 800 


Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile Pro Gln Ala Arg Leu 
                805                 810                 815     


Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu Ala Arg Asn Val Asn 
            820                 825                 830         


Ile Glu Lys Gly Asn Arg Thr Ala Tyr Asp Phe Val Lys Glu Lys Phe 
        835                 840                 845             


Gly Glu Ser Gly Thr Asp Asn Ser Leu Asp Cys Tyr Leu Asn Asn Ile 
    850                 855                 860                 


Glu Val Leu Phe Val Ser Gly Lys Ile Ser Lys Thr Lys Tyr Thr Lys 
865                 870                 875                 880 


Leu Lys Met Thr Glu Gln Asp Ile Pro Gly Gly Phe Ile Glu Arg Asp 
                885                 890                 895     


Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala Leu Ser Met Leu Asn 
            900                 905                 910         


Glu Ile Ser Arg Cys Val Val Ala Thr Ser Gly Ser Ile Thr Asp Lys 
        915                 920                 925             


Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn Trp 
    930                 935                 940                 


Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr Phe Glu Asp Lys Asp 
945                 950                 955                 960 


Gly Lys Gln Ile Gly Arg Ile Lys Asp Trp Ser Lys Arg Asn Asp His 
                965                 970                 975     


Arg His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Glu Val 
            980                 985                 990         


Phe Ile Gln Tyr Phe Asn Asn Lys  Asn Ala Ser Leu Asn  Pro Asn Thr 
        995                 1000                 1005             


Asn Glu  Tyr Ala Ile Lys Asn  Lys Tyr Phe Gln Asn  Gly Arg Ala 
    1010                 1015                 1020             


Ile Ala  Pro Ile Pro Leu Asn  Glu Phe Arg Ala Glu  Ala Lys Lys 
    1025                 1030                 1035             


His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys Ala Lys  Asn Lys Val 
    1040                 1045                 1050             


Ile Thr  Asn Asn Ile Asn Lys  Thr Lys Lys Arg Gly  Gly Val Asn 
    1055                 1060                 1065             


Thr Lys  Lys Gln Gln Thr Pro  Arg Gly Gln Leu His  Leu Glu Thr 
    1070                 1075                 1080             


Ile Tyr  Gly Cys Cys Arg Gln  Tyr Leu Thr Lys Glu  Glu Lys Val 
    1085                 1090                 1095             


Asn Ala  Ser Phe Asp Val Ser  Lys Ile Ala Thr Val  Ser Ser Ser 
    1100                 1105                 1110             


Val Tyr  Arg Asp Ala Leu Leu  Lys Arg Leu Gln Glu  His His Asn 
    1115                 1120                 1125             


Asp Pro  Lys Lys Ala Phe Ala  Gly Arg Asn Ser Leu  Asp Lys Lys 
    1130                 1135                 1140             


Pro Val  Trp Leu Asp Lys Glu  Gln Ile Arg Lys Val  Pro Glu Lys 
    1145                 1150                 1155             


Val Lys  Thr Val Thr Phe Glu  Thr Ile Tyr Thr Ile  Arg Lys Glu 
    1160                 1165                 1170             


Ile Ser  Pro Asp Leu Arg Val  Asp Lys Ile Ile Asp  Ser Gly Val 
    1175                 1180                 1185             


Arg Lys  Met Leu Thr Gly Arg  Leu Lys Glu Tyr Gly  Asn Asp Ala 
    1190                 1195                 1200             


Lys Lys  Ala Phe Ser Asn Leu  Asp Glu Lys Pro Ile  Trp Leu Asn 
    1205                 1210                 1215             


Lys Glu  Lys Gly Ile Pro Ile  Lys Arg Val Thr Ile  Ser Gly Ile 
    1220                 1225                 1230             


Ser Asn  Ala Gln Ser Leu His  Val Lys Lys Asp Lys  Asp Gly Lys 
    1235                 1240                 1245             


Pro Val  Leu Asp Gly Asn Gly  Arg Asn Ile Pro Val  Asp Phe Val 
    1250                 1255                 1260             


Asn Thr  Gly Asn Asn His His  Val Ala Val Tyr Arg  Arg Pro Val 
    1265                 1270                 1275             


Ile Asp  Lys Asn Gly Gln Met  Val Ile Asp Glu Asn  Gly Asn Leu 
    1280                 1285                 1290             


Lys Tyr  Glu Leu Glu Glu Val  Val Val Thr Phe Phe  Asp Ala Val 
    1295                 1300                 1305             


Thr Arg  Val Asn Leu Gly Gln  Pro Ile Ile Asp Lys  Asp Tyr Lys 
    1310                 1315                 1320             


Ala Ser  Glu Gly Trp Gln Phe  Leu Phe Ser Met Lys  Gln Asn Glu 
    1325                 1330                 1335             


Tyr Phe  Val Phe Pro Asn Lys  Lys Thr Gly Phe Asn  Pro Lys Glu 
    1340                 1345                 1350             


Ile Asp  Leu Leu Asp Ala Asp  Asn Tyr Gly Leu Ile  Ser Pro Asn 
    1355                 1360                 1365             


Leu Phe  Arg Val Gln Lys Phe  Thr His Lys Asn Tyr  Val Phe Arg 
    1370                 1375                 1380             


His His  Leu Glu Thr Thr Ile  Lys Asp Thr Ser Ser  Ile Leu Arg 
    1385                 1390                 1395             


Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser Lys Gly  Leu Asp Ser 
    1400                 1405                 1410             


Ile Val  Lys Val Arg Val Asn  His Ile Gly Gln Ile  Val Ser Val 
    1415                 1420                 1425             


Gly Glu  Tyr 
    1430     


<210>  1238
<211>  1432
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1238

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Ser Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Arg Asp Glu Ala Ser Ser Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Met Gln Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Asp Ala 
65                  70                  75                  80  


Leu Ile Lys Leu Leu Lys Lys Val Gly Phe Ile Ala Asp Gly Thr Ile 
                85                  90                  95      


Leu Ser Glu Gln Gly Asn Arg Ser Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Pro Asp Glu Glu Ile Ser Leu Gln Glu Leu Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ile 
    130                 135                 140                 


Lys Glu Lys Glu Ala Gly Thr Leu Ile Asp Gly Met Lys Val Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Asp Glu Asp Leu Thr Pro Gly Gln Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Glu Ser Gly Lys Lys Ser Leu Pro Asp Phe Tyr Ser Ser Asp Leu 
            180                 185                 190         


Gln Glu Glu Phe Asp Arg Ile Trp Asn Phe Gln Lys Gln Phe Asn Pro 
        195                 200                 205             


Lys Ala Phe Cys Asp Thr Ala Lys Glu Glu Ile Lys Gly Lys Asn Lys 
    210                 215                 220                 


Ser Gln Thr Trp Ala Ile Leu Ala Lys Tyr Phe Ile Trp Thr Asp Glu 
225                 230                 235                 240 


Met Val Val Trp Asn Glu Lys Glu Ser Lys Thr Glu Arg Val Glu Lys 
                245                 250                 255     


Glu Cys Lys Leu Val Gly Leu Lys Arg Thr Val Lys Lys Glu Glu Gln 
            260                 265                 270         


Arg Lys Glu Asn Tyr Arg Trp Arg Val Gln Ala Leu Thr Glu Gln Leu 
        275                 280                 285             


Asp Leu Gly Lys Val Ala Val Val Leu Gln Glu Ile Asn Gly Gln Ile 
    290                 295                 300                 


Ser Thr Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Val 
305                 310                 315                 320 


Leu Tyr Phe Asn Arg Gln Thr Val Gly Gln Tyr Gln Met Ala Glu Leu 
                325                 330                 335     


Glu Lys Asn Pro Asn Thr Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asp Lys Ile Trp Glu Lys Gln Ala Glu Phe 
        355                 360                 365             


His Lys Glu Leu Thr Val Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Asn Arg Glu Ile Met Val Glu Lys Asn Gly Lys Met 
                405                 410                 415     


Gln Ala Lys Val Val Gly Cys Lys Val Ile Pro Arg Ser His Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Thr Leu Asn Asn Val Glu Val Phe 
        435                 440                 445             


Ala Gly Glu Lys Lys Cys Lys Arg Lys Arg Thr Asp Ala Phe Pro Gly 
    450                 455                 460                 


Leu Phe Asp Gly Thr Glu Asp Thr Leu Met Val Glu Gly Trp Arg Tyr 
465                 470                 475                 480 


Leu Tyr Gln Glu Glu Lys Glu Ile Leu Ala Lys Glu Leu Ser Val Lys 
                485                 490                 495     


Lys Ser Leu Lys Lys Glu Tyr Ile Leu Lys Leu Leu Phe Asp Asn Pro 
            500                 505                 510         


Gln Asp Leu Asp Met Asn Phe Glu Gln Ile Glu Gly Asn Asp Thr Gly 
        515                 520                 525             


Asn Val Leu Phe Ser Ala Tyr Ser Lys Met Ile Glu Lys Tyr Gly Tyr 
    530                 535                 540                 


Glu Pro Leu Asp Phe Gly Lys Ser Thr Asp Glu Ile Ile Glu Arg Leu 
545                 550                 555                 560 


Gln Val Ile Phe Thr Asp Leu Gly Trp Asn Thr Asp Leu Leu Phe Val 
                565                 570                 575     


Asp Leu Asn Lys Glu Gly Lys Asp Leu Glu Lys Gln Pro Tyr Phe Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Lys Leu Val Glu Lys Leu Met Glu Leu Cys His Val Gly Lys 
    610                 615                 620                 


Glu Cys Ala Leu Glu Leu Val Asn Ile Ser Phe Gln Asn Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile Lys Lys Ile Leu Pro Tyr Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Gly Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Lys Ser Ser Leu Thr Lys Glu Glu Ile Glu Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Lys Leu Asp Ile Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Ile Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Ala Ile Ile Asp Thr 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Ser Ala Lys Glu Arg Asp Glu Leu Thr Lys Ala Ile Ala Lys Ser 
            740                 745                 750         


Thr Arg Glu His Glu Glu Ile Arg Lys Val Leu Gln Glu Glu Phe Gly 
        755                 760                 765             


Met Ile Asn Val Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Glu 
    770                 775                 780                 


Glu Leu Lys Asp Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Gln Tyr Ile 
785                 790                 795                 800 


Pro Lys Glu Lys Val Phe Ser Lys Glu Ile Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Leu Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Tyr Lys Ala Ile Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Tyr Gly Glu Lys Gly Leu Gln Gln Tyr Leu Asn Arg 
    850                 855                 860                 


Cys Glu Ser Leu Phe Lys Gly His Arg Thr Lys Leu Arg Lys Leu Lys 
865                 870                 875                 880 


Met Gly Gln Glu Asn Ile Pro Glu Gly Phe Ile Asp Arg Asp Leu Arg 
                885                 890                 895     


Asn Thr Gln Tyr Ile Ala Lys Lys Ala Leu Ala Met Leu Asn Glu Ile 
            900                 905                 910         


Cys Arg Arg Val Val Ala Thr Thr Gly Ser Ile Thr Asp Arg Leu Arg 
        915                 920                 925             


Glu Asp Trp Gln Leu Val Asp Val Leu Lys Glu Leu Asn Leu Pro Lys 
    930                 935                 940                 


Tyr Glu Thr Leu Gly Leu Val Glu Val Tyr Glu Asp Lys Asp Gly Arg 
945                 950                 955                 960 


Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His 
                965                 970                 975     


His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Asp Val Phe Ile 
            980                 985                 990         


Gln Tyr Phe Asn Asn Lys Asn Ala  Ala Trp Gln Ala Asp  Cys Lys Glu 
        995                 1000                 1005             


His Ser  Asp Ile Ile Gly Ile  Lys Thr Arg Tyr Phe  Glu Asn Gly 
    1010                 1015                 1020             


Arg Ala  Leu Pro Pro Met Pro  Leu Val Arg Phe Arg  Ala Glu Ala 
    1025                 1030                 1035             


Lys Ser  His Leu Glu Thr Leu  Leu Val Ser Ile Lys  Ala Lys Asn 
    1040                 1045                 1050             


Lys Val  Val Thr Asn Asn Val  Asn Cys Thr Lys Arg  Lys Gly Gly 
    1055                 1060                 1065             


Glu Asn  Lys Lys Leu Gln Gln  Thr Pro Arg Gly Gln  Leu His Leu 
    1070                 1075                 1080             


Glu Thr  Ile Tyr Gly Ser His  Arg Gln Tyr Val Met  Lys Glu Glu 
    1085                 1090                 1095             


Lys Val  Asn Ala Ser Phe Asp  Ser Ser Lys Ile Ala  Thr Val Cys 
    1100                 1105                 1110             


Lys Gln  Ala Tyr Arg Asn Ala  Leu Leu Glu Arg Leu  Ala Ala Phe 
    1115                 1120                 1125             


Gly Asn  Asp Pro Lys Lys Ala  Phe Thr Gly Lys Asn  Ser Leu Glu 
    1130                 1135                 1140             


Lys Asn  Pro Val Tyr Leu Asp  Arg Glu Gln Thr Ala  Arg Val Pro 
    1145                 1150                 1155             


Glu Arg  Val Arg Thr Val Glu  Leu Glu Thr Val Tyr  Thr Ile Arg 
    1160                 1165                 1170             


Lys Pro  Val Asp Pro Asn Leu  Ser Val Asp Lys Val  Val Asp Ala 
    1175                 1180                 1185             


Lys Val  Arg Ser Ile Leu Glu  Gln Arg Leu Arg Glu  Tyr Asp Gly 
    1190                 1195                 1200             


Glu Ala  Lys Arg Ala Phe Val  Asn Leu Asp Glu Asn  Pro Ile Trp 
    1205                 1210                 1215             


Leu Asn  Glu Glu Lys Gly Ile  Ala Ile Lys Arg Val  Thr Ile Arg 
    1220                 1225                 1230             


Gly Ile  Asn Lys Val Gln Ser  Leu His Gly Lys Lys  Asp Lys Asn 
    1235                 1240                 1245             


Gly Asn  Leu Ile Leu Asp Glu  Thr Gly Lys Lys Ile  Pro Val Asp 
    1250                 1255                 1260             


Phe Val  Asn Thr Gly Asn Asn  His His Val Ala Ile  Tyr Arg Lys 
    1265                 1270                 1275             


Pro Val  Leu Asp Lys Thr Gly  Gln Glu Met Leu Asp  Asn Glu Gly 
    1280                 1285                 1290             


Asn Pro  Val Tyr Glu Leu Asp  Glu Val Val Val Ser  Phe Phe Glu 
    1295                 1300                 1305             


Ala Val  Thr Arg Val Asn Leu  Gly Leu Pro Val Ile  Asp Lys Asn 
    1310                 1315                 1320             


Tyr Asn  Lys Ser Glu Gly Trp  Gln Phe Met Phe Thr  Met Lys Gln 
    1325                 1330                 1335             


Asn Glu  Tyr Phe Val Phe Pro  Asn Glu Lys Thr Gly  Phe Asp Pro 
    1340                 1345                 1350             


Lys Glu  Val Asp Leu Leu Asn  Pro Glu Asn Tyr Ala  Val Ile Ser 
    1355                 1360                 1365             


Pro Asn  Leu Phe Arg Val Gln  Lys Ile Thr Glu Lys  Asp Tyr Met 
    1370                 1375                 1380             


Phe Arg  His His Leu Glu Thr  Lys Val Val Asp Asp  Lys Phe Leu 
    1385                 1390                 1395             


Lys Glu  Ile Thr Trp Lys Arg  Ile Leu Thr Pro Asn  Lys Leu Glu 
    1400                 1405                 1410             


Gly Ile  Val Lys Val Arg Val  Asn His Ile Gly Gln  Ile Val Ser 
    1415                 1420                 1425             


Val Gly  Glu Tyr 
    1430         


<210>  1239
<211>  1432
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1239

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Ser Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Ser Asp Glu Val Ser Ser Ile Ile 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Met Gln Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Asp Ala 
65                  70                  75                  80  


Leu Ile Lys Leu Leu Lys Lys Val Gly Phe Ile Thr Asp Gly Thr Ile 
                85                  90                  95      


Leu Ser Glu Gln Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Pro Asp Glu Glu Ile Ser Leu Gln Glu Leu Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ile 
    130                 135                 140                 


Lys Glu Lys Glu Ala Gly Thr Leu Ile Asp Gly Met Glu Val Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Asp Glu Asp Leu Thr Pro Gly Gln Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Glu Ser Gly Lys Lys Ser Leu Pro Asp Phe Tyr Ser Ser Asp Leu 
            180                 185                 190         


Gln Glu Glu Phe Asp Arg Ile Trp Asn Phe Gln Lys Gln Phe Asn Pro 
        195                 200                 205             


Lys Ala Phe Cys Asp Thr Ala Lys Glu Glu Ile Lys Gly Lys Asn Lys 
    210                 215                 220                 


Ser Gln Thr Trp Ala Ile Leu Ala Lys Tyr Phe Ile Trp Thr Asp Glu 
225                 230                 235                 240 


Met Val Val Trp Asn Glu Lys Glu Ser Lys Thr Glu Arg Val Glu Lys 
                245                 250                 255     


Glu Cys Lys Leu Val Gly Leu Lys Arg Thr Val Lys Lys Glu Glu Gln 
            260                 265                 270         


Arg Lys Glu Asn Tyr Cys Trp Arg Val Gln Ala Leu Thr Glu Gln Leu 
        275                 280                 285             


Asp Leu Glu Lys Val Ala Val Val Leu Gln Glu Ile Asn Gly Gln Ile 
    290                 295                 300                 


Ser Thr Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Val 
305                 310                 315                 320 


Leu Tyr Phe Asn Arg Gln Thr Val Gly Gln Tyr Gln Met Ala Glu Leu 
                325                 330                 335     


Glu Lys Asn Pro Asn Thr Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asp Lys Ile Trp Glu Lys Gln Ala Glu Phe 
        355                 360                 365             


His Lys Glu Leu Thr Val Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Asn Arg Glu Ile Met Val Glu Lys Asn Gly Lys Met 
                405                 410                 415     


Gln Ala Lys Val Val Gly Cys Lys Val Ile Pro Arg Ser His Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Thr Leu Asn Asn Val Glu Val Phe 
        435                 440                 445             


Ala Gly Glu Lys Lys Cys Lys Arg Lys Arg Thr Asp Ala Phe Pro Gly 
    450                 455                 460                 


Leu Phe Asp Gly Thr Glu Asp Thr Leu Met Val Glu Gly Trp Arg Tyr 
465                 470                 475                 480 


Leu Tyr Gln Glu Glu Lys Glu Ile Leu Ala Lys Glu Leu Ser Val Lys 
                485                 490                 495     


Lys Ser Leu Lys Lys Glu Tyr Ile Leu Lys Leu Leu Phe Asp Asn Pro 
            500                 505                 510         


Gln Asp Leu Asp Met Asn Phe Glu Gln Ile Glu Gly Asn Asp Thr Gly 
        515                 520                 525             


Asn Val Leu Phe Ser Ala Tyr Ser Lys Met Ile Glu Lys Tyr Gly Tyr 
    530                 535                 540                 


Glu Pro Leu Asp Phe Gly Lys Pro Thr Asp Lys Ile Ile Glu Gln Leu 
545                 550                 555                 560 


Gln Val Ile Phe Ala Asp Leu Gly Trp Asn Thr Asp Leu Leu Phe Val 
                565                 570                 575     


Asp Leu Asn Lys Glu Gly Lys Asp Leu Glu Lys Gln Pro Tyr Phe Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Lys Leu Val Glu Lys Leu Met Glu Leu Cys His Val Gly Lys 
    610                 615                 620                 


Glu Cys Ala Leu Glu Leu Val Asn Ile Ser Phe Gln Asn Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile Lys Lys Ile Leu Pro Tyr Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Gly Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Lys Ser Ser Leu Thr Lys Glu Glu Ile Glu Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Lys Leu Asp Ile Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Ala Ile Ile Asp Thr 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Ser Ala Lys Glu Arg Glu Glu Leu Thr Lys Ala Ile Ala Lys Ser 
            740                 745                 750         


Thr Arg Glu His Glu Glu Ile Arg Lys Val Leu Gln Glu Glu Phe Gly 
        755                 760                 765             


Met Ile Asn Val Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Glu 
    770                 775                 780                 


Glu Leu Lys Asp Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Gln Tyr Ile 
785                 790                 795                 800 


Pro Lys Glu Lys Val Phe Ser Lys Glu Ile Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Leu Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Tyr Lys Ala Ile Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Tyr Gly Glu Lys Gly Leu Gln Gln Tyr Leu Asn Arg 
    850                 855                 860                 


Cys Glu Ser Leu Phe Lys Gly Gln Arg Thr Lys Leu Arg Lys Leu Lys 
865                 870                 875                 880 


Met Gly Gln Glu Asn Ile Pro Glu Gly Phe Ile Asp Arg Asp Leu Arg 
                885                 890                 895     


Asn Thr Gln Tyr Ile Ala Lys Lys Ala Leu Ala Met Leu Asn Glu Ile 
            900                 905                 910         


Cys Arg Arg Val Val Ala Thr Thr Gly Ser Ile Thr Asp Arg Leu Arg 
        915                 920                 925             


Glu Asp Trp Gln Leu Val Asp Val Leu Lys Glu Leu Asn Leu Pro Lys 
    930                 935                 940                 


Tyr Glu Thr Leu Gly Leu Val Glu Val Tyr Glu Asp Lys Asp Gly Arg 
945                 950                 955                 960 


Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His 
                965                 970                 975     


His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Asp Val Phe Ile 
            980                 985                 990         


Gln Tyr Phe Asn Asn Lys Asn Ala  Ala Trp Gln Ala Asp  Cys Lys Glu 
        995                 1000                 1005             


His Ser  Asp Ile Ile Gly Ile  Lys Thr Arg Tyr Phe  Glu Asn Gly 
    1010                 1015                 1020             


Arg Ser  Leu Pro Pro Met Pro  Leu Val Arg Phe Arg  Ala Glu Ala 
    1025                 1030                 1035             


Lys Ser  His Leu Glu Thr Leu  Leu Val Ser Ile Lys  Ala Lys Asn 
    1040                 1045                 1050             


Lys Val  Val Thr Asn Asn Val  Asn Cys Thr Lys Arg  Lys Gly Gly 
    1055                 1060                 1065             


Glu Asn  Lys Lys Leu Gln Gln  Ser Pro Arg Gly Gln  Leu His Leu 
    1070                 1075                 1080             


Glu Thr  Ile Tyr Gly Ser His  Arg Gln Tyr Val Thr  Lys Glu Glu 
    1085                 1090                 1095             


Lys Val  Asn Ala Ser Phe Asp  Ser Ser Lys Ile Ala  Thr Val Cys 
    1100                 1105                 1110             


Lys Gln  Ala Tyr Arg Asn Ala  Leu Leu Glu Arg Leu  Ala Ala Phe 
    1115                 1120                 1125             


Gly Asn  Asp Pro Lys Lys Ala  Phe Thr Gly Lys Asn  Ser Leu Glu 
    1130                 1135                 1140             


Lys Asn  Pro Val Tyr Leu Asn  Arg Glu Gln Thr Ala  Arg Val Pro 
    1145                 1150                 1155             


Glu Arg  Val Arg Thr Val Glu  Leu Glu Thr Val Tyr  Thr Ile Arg 
    1160                 1165                 1170             


Lys Pro  Val Asp Pro Asn Leu  Ser Val Asp Lys Val  Val Asp Ala 
    1175                 1180                 1185             


Lys Val  Arg Ser Ile Leu Glu  Gln Arg Leu Arg Glu  Tyr Asp Gly 
    1190                 1195                 1200             


Glu Ala  Lys Arg Ala Phe Val  Asn Leu Asp Glu Asn  Pro Ile Trp 
    1205                 1210                 1215             


Leu Asn  Glu Glu Lys Gly Ile  Ala Ile Lys Arg Val  Thr Ile Arg 
    1220                 1225                 1230             


Gly Ile  Asn Lys Val Gln Ser  Leu His Gly Lys Lys  Asp Lys Asn 
    1235                 1240                 1245             


Gly Asn  Leu Ile Leu Asp Glu  Thr Gly Lys Lys Ile  Pro Val Asp 
    1250                 1255                 1260             


Phe Val  Asn Thr Gly Asn Asn  His His Val Ala Ile  Tyr Arg Lys 
    1265                 1270                 1275             


Pro Val  Leu Asn Lys Thr Gly  Gln Glu Met Leu Asp  Asn Glu Gly 
    1280                 1285                 1290             


Asn Pro  Val Tyr Glu Leu Asp  Glu Val Val Val Ser  Phe Phe Glu 
    1295                 1300                 1305             


Ala Val  Thr Arg Val Asn Leu  Gly Leu Pro Val Ile  Asp Lys Asn 
    1310                 1315                 1320             


Tyr Asn  Lys Ser Glu Gly Trp  Gln Phe Met Phe Thr  Met Lys Gln 
    1325                 1330                 1335             


Asn Glu  Tyr Phe Val Phe Pro  Asn Glu Lys Thr Arg  Phe Asp Pro 
    1340                 1345                 1350             


Lys Glu  Val Asp Leu Leu Asn  Pro Glu Asn Tyr Ala  Val Ile Ser 
    1355                 1360                 1365             


Pro Asn  Leu Phe Arg Val Gln  Lys Ile Thr Glu Lys  Asp Tyr Met 
    1370                 1375                 1380             


Phe Arg  His His Leu Glu Thr  Lys Val Val Asp Asp  Lys Phe Leu 
    1385                 1390                 1395             


Lys Glu  Ile Thr Trp Lys Arg  Ile Leu Thr Pro Asn  Lys Leu Glu 
    1400                 1405                 1410             


Gly Ile  Val Lys Val Arg Val  Asn His Ile Gly Gln  Ile Val Ser 
    1415                 1420                 1425             


Val Gly  Glu Tyr 
    1430         


<210>  1240
<211>  1432
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1240

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Phe 
1               5                   10                  15      


Ala His Ile Val Glu Asp Glu Asn Lys Glu Lys Ser Glu Ile Lys Glu 
            20                  25                  30          


Leu Gly Val Arg Ile Val Ser Leu Thr Thr Asp Glu Gln Ser Asp Phe 
        35                  40                  45              


Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asn Arg Thr Leu Lys His 
    50                  55                  60                  


Gly Ala Arg Leu Asn Leu Asp Arg Tyr Gln Gln Arg Arg Lys Tyr Leu 
65                  70                  75                  80  


Ile Asp Leu Leu His Lys Ala Asn Leu Ile Thr Pro Ser Ser Ile Leu 
                85                  90                  95      


Ala Glu Asn Gly Lys Asn Thr Thr His Ser Thr Trp Gln Leu Arg Ala 
            100                 105                 110         


Lys Ala Val Thr Glu Arg Ile Glu Lys Glu Glu Phe Ala Arg Ile Leu 
        115                 120                 125             


Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala Lys 
    130                 135                 140                 


Thr Glu Asp Glu Gly Gln Ala Ile Asp Gly Met Ala Ile Ala Lys Arg 
145                 150                 155                 160 


Leu Tyr Asp Glu Asn Leu Thr Pro Gly Gln Leu Ser Leu Gln Leu Leu 
                165                 170                 175     


Gln Gln Asn Lys Lys Leu Leu Pro Asp Phe Tyr Arg Ser Asp Leu Gln 
            180                 185                 190         


Lys Glu Phe Asp Leu Val Trp Asn Phe Gln Lys Gln Phe Tyr Pro Asp 
        195                 200                 205             


Ile Leu Thr Asp Ile Phe Tyr Lys Glu Leu Gln Gly Lys Gly Lys Asp 
    210                 215                 220                 


Ala Thr Ser Lys Ala Phe Ser Lys Arg Tyr His Phe Asp Thr Ala Glu 
225                 230                 235                 240 


Asn Lys Gly Ser Lys Glu Ser Val Arg Leu Gln Ala Tyr Gln Trp Arg 
                245                 250                 255     


Ala Glu Ala Val Ser Lys Gln Leu Ser Lys Glu Glu Val Ala Tyr Val 
            260                 265                 270         


Leu Thr Glu Ile Asn Asn Asn Leu Asn Asn Ala Ser Gly Tyr Leu Gly 
        275                 280                 285             


Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Arg Gln Thr Val 
    290                 295                 300                 


Gly Gln Tyr Leu Tyr Ala Gln Leu Gln Glu Asn Arg His Asn Ser Leu 
305                 310                 315                 320 


Lys Asn Lys Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Arg 
                325                 330                 335     


Ile Trp Glu Thr Gln Ala Leu Phe His Lys Glu Leu Thr Asp Glu Leu 
            340                 345                 350         


Lys Lys Gln Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Pro Lys 
        355                 360                 365             


Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Lys Glu Ile 
    370                 375                 380                 


Glu Ile Glu Lys Asp Gly Lys Thr Ile Thr Lys Asn Ile Gly Ala Arg 
385                 390                 395                 400 


Val Val Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln 
                405                 410                 415     


Ile Leu Asn Asn Val Ile Cys Lys Arg Lys Gly Ile Arg Lys Lys Lys 
            420                 425                 430         


Ile Ser Ala Lys Thr Thr Gln Leu Asp Leu Leu Asn Glu Ser Ser Gln 
        435                 440                 445             


Thr Ile Phe Ser Leu Asp Met Glu Cys Lys Gln Leu Leu Phe Asp Glu 
    450                 455                 460                 


Leu Asn Leu Lys Gly Asp Leu Lys Ser Asp Lys Val Leu Lys Leu Leu 
465                 470                 475                 480 


Gly Tyr Ser Pro Gln Glu Trp Glu Ile Asn Tyr Asn Gln Leu Glu Gly 
                485                 490                 495     


Asn Arg Thr Gln Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Val Glu 
            500                 505                 510         


Met Glu Ala His Asp Val Lys Asp Ile Leu Gln Ile Lys Ser Thr Lys 
        515                 520                 525             


Asp Asp Trp Ser Leu Asp Glu Ser Pro Leu Ser Ala Ser Glu Ile Arg 
    530                 535                 540                 


Glu Ser Val Lys Ala Ile Phe Gln Ala Leu Gly Ile Cys Thr Lys Ile 
545                 550                 555                 560 


Leu Tyr Phe Asp Pro Leu Leu Pro Val Lys Asp Phe Glu Glu Gln Asp 
                565                 570                 575     


Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asp Ser 
            580                 585                 590         


Thr Ser Gly Asn Glu Thr Leu Tyr Arg Ile Leu Glu Lys Lys Tyr Ala 
        595                 600                 605             


Phe Lys Arg Glu His Ala Arg Ile Leu Ala Asn Val Ala Leu Gln Asp 
    610                 615                 620                 


Asp Tyr Gly Ser Leu Ser Thr Lys Ala Ile Arg Lys Ile Tyr Pro Asn 
625                 630                 635                 640 


Ile Lys Glu Asn Gln Tyr Ser Thr Ala Cys Glu Met Ala Gly Tyr Lys 
                645                 650                 655     


His Ser Lys Leu Ser Leu Thr Thr Glu Glu Leu Glu Ala Arg Glu Leu 
            660                 665                 670         


Lys Asn Ile Ile Pro Leu Leu Lys Lys Asn Ala Leu Arg Asn Pro Val 
        675                 680                 685             


Val Glu Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Ala Leu Ile 
    690                 695                 700                 


Glu Lys Asn Ser Glu Arg Asp Ala Glu Gly Lys Ile Thr Lys Tyr Phe 
705                 710                 715                 720 


His Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn 
                725                 730                 735     


Ala Gln Lys Arg Tyr Glu Met Thr Gln Asn Ile Asn Lys Ala Lys Ser 
            740                 745                 750         


Glu His Gln Lys Ile Ser Glu Ile Leu Gln Lys Glu Phe Gly Ile Lys 
        755                 760                 765             


Asn Pro Thr Lys Ser Asp Ile Ile Arg Tyr Arg Leu Tyr Lys Glu Leu 
    770                 775                 780                 


Glu His Asn Gly Tyr Lys Glu Leu Tyr Thr Asn Thr Tyr Ile Ala Pro 
785                 790                 795                 800 


Asp Val Leu Phe Ser Lys Asn Ile Asp Ile Glu His Ile Val Pro Lys 
                805                 810                 815     


Ala Arg Val Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Ala Phe Lys 
            820                 825                 830         


Asp Phe Asn Ile Lys Lys Gly Glu Ser Thr Ala Phe Asp Tyr Ile Thr 
        835                 840                 845             


Ser Leu Lys Asn Glu Glu Lys Leu Asn Gln Tyr Leu Ala Arg Val Glu 
    850                 855                 860                 


Asn Ala Tyr Lys Thr Lys Asn Ile Ser Pro Ala Lys Tyr Lys Asn Leu 
865                 870                 875                 880 


Leu Lys Lys Ala Ser Glu Ile Gly Asp Gly Phe Ile Asn Arg Asp Leu 
                885                 890                 895     


Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Phe Gln 
            900                 905                 910         


Val Thr Lys Asn Val Leu Ser Thr Ser Gly Ser Ile Thr Asp Arg Leu 
        915                 920                 925             


Arg Glu Asp Trp Ser Leu Val Asp Val Met Lys Glu Leu Asn Met Pro 
    930                 935                 940                 


Lys Tyr Gln Ser Leu Gly Leu Thr Glu Val Glu Glu Arg Lys Asp Gly 
945                 950                 955                 960 


Asn Lys Val Thr Val Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg 
                965                 970                 975     


His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Pro Ser Tyr 
            980                 985                 990         


Ile Gln Tyr Leu Asn His Leu Asn  Ala Arg Lys Asp Glu  Asn Asn Lys 
        995                 1000                 1005             


Asn Tyr  Ser Val Ile Leu Ala  Ile Glu Lys Lys Glu  Thr Ile Lys 
    1010                 1015                 1020             


Val Pro  Thr Asn Asn Gly Lys  Asn Lys Arg Val Phe  Ile Glu Pro 
    1025                 1030                 1035             


Ile Pro  Asn Phe Arg Gln Val  Ala Lys Lys His Leu  Glu Glu Ile 
    1040                 1045                 1050             


Phe Ile  Ser His Lys Ala Lys  Asn Lys Val Val Thr  Lys Asn Thr 
    1055                 1060                 1065             


Asn Lys  Pro Ala Gly Thr Asn  Lys Gln Gln Ile Thr  Leu Thr Pro 
    1070                 1075                 1080             


Arg Gly  Gln Leu His Lys Glu  Thr Ile Tyr Gly Lys  Tyr Gln Tyr 
    1085                 1090                 1095             


Tyr Val  Asn Lys Glu Glu Lys  Val Gly Val Lys Phe  Asp Lys Glu 
    1100                 1105                 1110             


Thr Ile  Ala Lys Val Ser Asn  Pro Leu Tyr Arg Glu  Ala Leu Leu 
    1115                 1120                 1125             


Lys Arg  Leu Gln Ala Asn Asp  Asn Asp Pro Lys Lys  Ala Phe Thr 
    1130                 1135                 1140             


Gly Lys  Asn Ala Leu Ser Lys  Asn Pro Ile Tyr Leu  Asp Glu Ala 
    1145                 1150                 1155             


Lys Thr  Lys Thr Leu Pro Glu  Lys Val Asn Leu Thr  Tyr Leu Glu 
    1160                 1165                 1170             


Glu Asp  Phe Ser Val Arg Lys  Lys Ile Val Pro Lys  Asn Pro Thr 
    1175                 1180                 1185             


Ser Lys  Glu Phe Val Pro Asn  Leu Lys Ser Ile Glu  Lys Val Val 
    1190                 1195                 1200             


Asp Glu  Gly Val Arg Asn Ile  Leu Ile Glu Arg Leu  Arg Val Tyr 
    1205                 1210                 1215             


Asn Asn  Asp Pro Glu Lys Ala  Phe Val Asp Leu Glu  Lys Asn Pro 
    1220                 1225                 1230             


Ile Trp  Leu Asn Lys Glu Lys  Gly Ile Ala Ile Lys  Arg Val Thr 
    1235                 1240                 1245             


Ile Ser  Gly Val Asn Asn Ala  Gln Pro Leu His Ile  Gly Lys Asp 
    1250                 1255                 1260             


His Leu  Gly Lys Thr Thr Leu  Asn Lys Glu Gly Lys  Glu Ile Pro 
    1265                 1270                 1275             


Val Asp  Tyr Val Ser Thr Gly  Asn Asn His His Val  Ala Ile Tyr 
    1280                 1285                 1290             


Arg Asp  Lys Glu Gly Asn Leu  Gln Glu Gln Ile Val  Ser Phe Phe 
    1295                 1300                 1305             


Glu Ala  Val Val Arg Thr Gln  Gln Gly Leu Pro Ile  Ile Asp Lys 
    1310                 1315                 1320             


Thr Tyr  Lys Gln Glu Glu Gly  Trp Gln Phe Leu Phe  Thr Met Lys 
    1325                 1330                 1335             


Gln Asn  Glu Met Phe Val Phe  Pro Asn Glu Ala Thr  Gly Phe Asn 
    1340                 1345                 1350             


Pro Ala  Glu Ile Asp Leu Leu  Asp Pro Lys Asn Lys  Lys Gln Ile 
    1355                 1360                 1365             


Ser Pro  Asn Leu Phe Arg Val  Gln Lys Ile Ala Thr  Lys Asp Tyr 
    1370                 1375                 1380             


Phe Phe  Arg His His Leu Glu  Thr Asn Val Glu Thr  Asp Asn Val 
    1385                 1390                 1395             


Leu Lys  Asn Thr Thr Trp Lys  Arg Glu Gly Leu Ser  Gly Leu Lys 
    1400                 1405                 1410             


Asp Ile  Val Lys Val Arg Ile  Asn His Leu Gly Asp  Ile Val Ser 
    1415                 1420                 1425             


Ile Gly  Glu Tyr 
    1430         


<210>  1241
<211>  1433
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1241

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Tyr 
1               5                   10                  15      


Ala Gln Val Asn Glu Ser Glu Asp Phe Lys Asp Ser Ser Ile Glu Arg 
            20                  25                  30          


Ile Gly Val Arg Val Asn Pro Leu Ser Val Asp Glu Gln Thr Asn Phe 
        35                  40                  45              


Glu Lys Gly Lys Pro Ile Ser Ile Asn Ala Asp Arg Thr Leu Lys Ser 
    50                  55                  60                  


Gly Ala Arg Arg Thr Leu Asp Arg Tyr Gln Asp Arg Arg Ser Asn Leu 
65                  70                  75                  80  


Ile Asp Ala Leu Val Lys Ser Val Ile Ile Thr Glu Asp Thr Val Leu 
                85                  90                  95      


Ala Glu Asp Gly Lys Asn Thr Thr His Ser Thr Phe Ala Leu Arg Ala 
            100                 105                 110         


Lys Ser Ala Ser Glu Lys Val Glu Lys Ala Glu Phe Ala Arg Ile Leu 
        115                 120                 125             


Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Thr Asn 
    130                 135                 140                 


Asn Asn Asp Asp Glu Gly Gln Ala Ile Asp Gly Met Ala Ile Ala Lys 
145                 150                 155                 160 


Lys Leu Tyr Asp Glu Asn Leu Thr Pro Gly Gln Leu Ala Tyr Gln Leu 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Tyr Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Ala Glu Phe Asp Lys Ile Trp Thr Phe Gln Lys Gln Phe Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Glu Leu Gln Lys Glu Leu Ala Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ser Ala Ile Phe Trp Lys Lys Tyr Asp Phe Asn Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Thr Arg Glu Glu Lys Lys Leu Lys Ala Tyr Glu Trp 
                245                 250                 255     


Arg Ser Thr Ser Val Asp Lys Lys Leu Gln Lys Glu Glu Val Ala Tyr 
            260                 265                 270         


Val Ile Thr Glu Ile Asn Asn Asn Leu Thr Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr 
    290                 295                 300                 


Val Gly Gln Tyr Leu Phe Lys Gln Ile Gln Lys Asn Pro His Thr Lys 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Phe Asp Glu Phe Glu 
                325                 330                 335     


Ile Ile Trp Glu Thr Gln Ala Lys Phe His Pro Glu Leu Thr Glu Glu 
            340                 345                 350         


Leu Lys Asn Lys Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Lys Leu 
        355                 360                 365             


Lys Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Arg Lys Glu 
    370                 375                 380                 


Val Glu Ile Glu Lys Gly Gly Arg Arg Met Lys Lys Thr Ile Gly Leu 
385                 390                 395                 400 


Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp 
                405                 410                 415     


Gln Val Leu Asn Asn Val Leu Ile Arg Lys Lys Gly Ser Lys Lys Arg 
            420                 425                 430         


Arg Val Lys Glu Pro Asp Gln Leu Asn Leu Val Glu Gln Glu Gln Glu 
        435                 440                 445             


Val Met Phe Phe Asp Met Gln Thr Lys Gln Thr Leu Phe Asp Glu Leu 
    450                 455                 460                 


Asn Val Lys Gly Asn Leu Lys Ala Lys Arg Ile Ile Glu Leu Ile Gly 
465                 470                 475                 480 


Gln Asp Ala Asn Asp Trp Glu Ile Asn Tyr Ser Glu Ile Glu Gly Asn 
                485                 490                 495     


Arg Thr Asn Lys Ala Leu Tyr Asp Ala Tyr Leu Lys Ile Leu Asp Leu 
            500                 505                 510         


Glu Gly Tyr Asp Thr Lys Ala Leu Leu Asp Val Lys Thr Ser Lys Asp 
        515                 520                 525             


Glu Val Glu Leu Asp Asp Ile Asn Ile Ser Gly Pro Glu Val Lys Asp 
    530                 535                 540                 


Leu Val Lys Arg Ile Phe Glu Ser Leu Glu Ile Asp Thr Gln Ile Leu 
545                 550                 555                 560 


Glu Phe Asp Ala Glu Leu Asp Gly Glu Val Phe Glu Lys Gln Ser Ser 
                565                 570                 575     


Tyr Leu Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp Ser Pro 
            580                 585                 590         


Ser Gly Asn Glu Lys Leu Tyr Lys Leu Leu Lys Gln Lys Phe Gly Phe 
        595                 600                 605             


Lys Lys Glu His Ala Gln Ile Leu Ala Ser Ile Thr Leu Ser Asp Asp 
    610                 615                 620                 


Tyr Gly Ser Leu Ser Ala Lys Ala Met Arg Lys Ile Tyr Pro Tyr Ile 
625                 630                 635                 640 


Arg Glu Gln Thr Phe Asp Lys Ala Ala Ser Leu Ala Gly Tyr Arg His 
                645                 650                 655     


Ser Ala Ser Ser Leu Thr Lys Glu Glu Ile Ala Asn Arg Pro Leu Lys 
            660                 665                 670         


Asp Lys Leu Glu Leu Ile Lys Lys Asn Ser Leu Arg Asn Pro Val Val 
        675                 680                 685             


Glu Lys Ile Leu Asn Gln Val Val Asn Ile Val Asn Ser Leu Ile Glu 
    690                 695                 700                 


Ala Asn Ser Glu Lys Asp Ser Asp Gly Asn Ile Ile Ser Tyr Phe Lys 
705                 710                 715                 720 


Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Asp Leu Lys Lys Asn Ala 
                725                 730                 735     


Lys Glu Arg Ala Glu Met Thr Leu Asn Ile Asn Ala Ala Lys Ile Val 
            740                 745                 750         


His Glu Lys Ile Phe Lys Leu Leu Gln Ala Glu Phe Gly Val Lys Asn 
        755                 760                 765             


Pro Thr Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Glu Glu Leu Lys 
    770                 775                 780                 


Asn Asn Gly Tyr Lys Asp Leu Tyr Thr Asn Ile Tyr Ile Pro Arg Glu 
785                 790                 795                 800 


Ile Leu Phe Ser Lys Gln Ile Asp Ile Glu His Ile Ile Pro Gln Ser 
                805                 810                 815     


Lys Met Phe Asp Asp Ser Phe Ser Asn Lys Thr Ile Val Phe Arg Lys 
            820                 825                 830         


Asp Asn Leu Asp Lys Gly Asn Ser Thr Ala Phe Asp Tyr Ile Asp Ser 
        835                 840                 845             


Arg Phe Gly Lys Asp Lys Leu Thr Asp Phe Glu Gln Arg Ile Glu Phe 
    850                 855                 860                 


Leu Phe Glu Gln Gly Lys Lys Asn Lys Glu Glu Gly Ile Ser Lys Ala 
865                 870                 875                 880 


Lys Tyr Gln Lys Leu Leu Lys Arg Glu Ala Asp Ile Gly Asp Gly Phe 
                885                 890                 895     


Ile Glu Arg Asp Leu Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Arg 
            900                 905                 910         


Asn Leu Leu Tyr Glu Ile Cys Arg Ile Val Thr Pro Thr Thr Gly Ser 
        915                 920                 925             


Ile Thr Asp Arg Leu Arg Glu Asp Trp Asp Leu Val Asn Ile Met Gln 
    930                 935                 940                 


Glu Leu Asn Leu Asp Lys Phe Lys Lys Leu Gly Leu Thr Glu Met Val 
945                 950                 955                 960 


Glu Lys Lys Asp Gly Ser Phe Lys Glu Arg Ile Val Asp Trp Ser Lys 
                965                 970                 975     


Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Ile Ala Phe 
            980                 985                 990         


Thr Lys His Ser His Ile Gln Tyr  Leu Asn Tyr Leu Asn  Ala Arg Lys 
        995                 1000                 1005             


Asn Glu  Asn His Lys Gln His  His Asn Ile Ile Gly  Ile Glu Arg 
    1010                 1015                 1020             


Lys Glu  Thr Glu Phe Val Ile  Asp Asp Leu Gly Asn  Lys Lys Arg 
    1025                 1030                 1035             


Lys Phe  His Leu Pro Leu Pro  Asn Phe Arg Glu Glu  Ala Lys Lys 
    1040                 1045                 1050             


His Leu  Glu Asn Val Leu Val  Ser Phe Lys Ala Lys  Asn Lys Val 
    1055                 1060                 1065             


Val Thr  Lys Asn Lys Asn Lys  Thr Ala Ser Ser Lys  Gly Glu Lys 
    1070                 1075                 1080             


Ile Lys  Val Glu Leu Thr Pro  Arg Gly Gln Leu His  Lys Glu Thr 
    1085                 1090                 1095             


Val Tyr  Gly Lys Tyr Lys Phe  Tyr Val Ser Lys Glu  Glu Arg Ile 
    1100                 1105                 1110             


Gly Gly  Lys Phe Asp Gln Arg  Thr Ile Glu Arg Val  Ala Asn Ser 
    1115                 1120                 1125             


Thr His  Lys Arg Leu Leu Leu  Gln Arg Leu Leu Glu  Asn Asp Asn 
    1130                 1135                 1140             


Asp Ala  Lys Lys Ala Phe Thr  Gly Lys Asn Ala Leu  Ala Lys Asn 
    1145                 1150                 1155             


Pro Ile  Tyr Leu Asp Asp Thr  Lys Thr Leu Thr Leu  Pro Glu Ile 
    1160                 1165                 1170             


Val Lys  Leu Thr Trp Leu Glu  Glu Asp Tyr Ser Ile  Arg Lys Asp 
    1175                 1180                 1185             


Ile Thr  Pro Glu Asn Phe Lys  Asp Ile Lys Val Ile  Asp Lys Ile 
    1190                 1195                 1200             


Leu Asp  Glu Gly Val Lys Arg  Ile Leu Lys Glu Arg  Leu Ala Lys 
    1205                 1210                 1215             


His Asn  Asn Asn Pro Lys Glu  Ala Phe Ser Asp Leu  Glu Lys Asn 
    1220                 1225                 1230             


Pro Ile  Trp Leu Asn Glu Lys  Lys Ser Ile Ser Ile  Lys Lys Val 
    1235                 1240                 1245             


Lys Ile  Ser Gly Ile Asn Asn  Ala Glu Ala Leu His  Tyr Lys Lys 
    1250                 1255                 1260             


Asn His  Leu Gly Ile Glu Ile  Leu Asp Asp Glu Lys  Lys Arg Ile 
    1265                 1270                 1275             


Pro Val  Asp Phe Val Ser Thr  Gly Asn Asn His His  Val Ala Ile 
    1280                 1285                 1290             


Tyr Arg  Asp Glu Lys Gly Asn  Leu Gln Glu Asp Val  Val Ser Phe 
    1295                 1300                 1305             


Phe Glu  Ala Val Ala Arg Val  Asn Gln Asn Leu Pro  Ile Ile Asp 
    1310                 1315                 1320             


Lys Thr  Leu Lys Ala Tyr Leu  Gly Trp Thr Phe Leu  Phe Thr Met 
    1325                 1330                 1335             


Lys Gln  Asn Glu Tyr Phe Ile  Phe Pro Asn Lys Gln  Thr Gly Phe 
    1340                 1345                 1350             


Asn Pro  Thr Glu Ile Asp Leu  Leu Asp Ser Met Asn  Asn Lys Leu 
    1355                 1360                 1365             


Ile Ser  Pro Asn Leu Phe Arg  Val Gln Lys Phe Thr  Val Lys Asp 
    1370                 1375                 1380             


Tyr Phe  Phe Arg His His Leu  Glu Thr Asn Val Glu  Asp Asp Ser 
    1385                 1390                 1395             


Lys Leu  Lys Asn Thr Thr Trp  Lys Arg Glu Gly Leu  Gly Gly Ile 
    1400                 1405                 1410             


Gly Asn  Ile Val Lys Val Arg  Ile Asn His Ile Gly  Gln Ile Val 
    1415                 1420                 1425             


Lys Ile  Gly Glu Tyr 
    1430             


<210>  1242
<211>  1434
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1242

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Tyr Val Lys Gln Ala Gln Asn Asp Ser Glu Lys Ser Glu Ile Ala 
            20                  25                  30          


Glu Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Pro Ile Ser Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Gln Glu 
65                  70                  75                  80  


Leu Ile Glu Leu Leu Lys Asp Tyr Gln Val Ile Ser Asp Lys Thr Ile 
                85                  90                  95      


Leu Ala Glu Asp Gly Lys Glu Thr Thr Phe Glu Thr Leu Arg Leu Arg 
            100                 105                 110         


Val Lys Ser Ala Lys Glu Gln Val Ser Leu Glu Glu Leu Ala Arg Ile 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Glu Asp Glu Gly Gln Leu Ile Asp Gly Met Glu Ile Ala Arg 
145                 150                 155                 160 


Arg Leu Tyr Asp Glu Asn Leu Thr Thr Gly Gln Leu Ser Leu Gln Leu 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Ser Leu Pro Asp Tyr Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln His Glu Leu Asp Lys Val Trp Asn Phe Gln Lys Gln Phe His Pro 
        195                 200                 205             


Asp Ile Leu Thr Asp Glu Phe Tyr Thr Ser Ile Gln Gly Lys Gly Gln 
    210                 215                 220                 


Lys Ala Thr Arg Glu Ser Phe Ser Ser Lys Tyr Gly Ile Asp Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Ser Arg Glu Glu Lys Lys Leu Gln Ala Tyr Gln Trp 
                245                 250                 255     


Arg Ala Asp Ala Thr His Lys Glu Leu Thr Lys Glu Glu Leu Ala Phe 
            260                 265                 270         


Val Ile Thr Glu Ile Asn Asn Asn Ile Asn Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Ile Phe Ala Asn Glu Thr 
    290                 295                 300                 


Val Gly Glu Tyr Leu Trp Lys Gln Ile Gln Asn Asp Pro His Thr Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Phe Asp Glu Phe Glu 
                325                 330                 335     


Arg Ile Trp Glu Thr Gln Ser Gly Phe Tyr Pro Gln Ile Leu Thr Glu 
            340                 345                 350         


Ser Leu Lys Asn Glu Leu Arg Asp Ile Leu Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Ala Leu Val Ser Ile Cys Glu Phe Glu Ser Lys 
    370                 375                 380                 


Glu Ile Pro Leu Lys Asp Lys Gln Gly Asn Pro Ile Ser Lys Asn Gly 
385                 390                 395                 400 


Ile Pro Val Thr Lys Leu Val Gly Pro Lys Val Ala Pro Lys Ser Ser 
                405                 410                 415     


Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln Asn Leu Asn Asn Val Leu 
            420                 425                 430         


Val Arg Pro Lys Gly Ser Arg Lys Arg Lys Val Thr Thr Asn Gln Gln 
        435                 440                 445             


Ser Thr Leu Leu Glu Gly Gln Glu Pro Val Phe Glu Leu Asn Leu Glu 
    450                 455                 460                 


Gln Lys Glu Leu Leu Phe Glu Glu Leu Asn Leu Lys Gly Asn Leu Lys 
465                 470                 475                 480 


Thr Asp Tyr Cys Leu Glu Leu Ile Gly Tyr Ser Ala Lys Glu Trp Glu 
                485                 490                 495     


Met Asn Tyr Pro Thr Leu Glu Gly Asn Lys Thr Asn Lys Ala Leu Tyr 
            500                 505                 510         


Glu Ala Tyr Leu Lys Ile Ile Asp Ser Glu Gly Tyr Asp Ala Lys Lys 
        515                 520                 525             


Leu Leu Lys Val Lys Thr Asp Lys Asp Asp Ile Asn Leu Asp Asp Ile 
    530                 535                 540                 


Lys Val Ser Ala Ser Glu Ile Lys Thr Met Ile Ser Asp Ile Phe Asn 
545                 550                 555                 560 


Glu Leu Gly Ile Asn Thr Lys Ile Leu Asp Phe Asp Ala Glu Leu Glu 
                565                 570                 575     


Arg Lys Gln Phe Glu Ala Gln Ala Ser Tyr Arg Leu Trp His Leu Leu 
            580                 585                 590         


Tyr Ser Tyr Glu Gly Asp Asp Ser Lys Ser Gly Asn Glu Leu Leu Tyr 
        595                 600                 605             


Arg Leu Leu Glu Asp Lys Phe Gly Phe Lys Arg Glu His Ser Lys Thr 
    610                 615                 620                 


Leu Ala Asn Val Ser Leu Leu Ser Asp Tyr Gly Asn Leu Ser Thr Lys 
625                 630                 635                 640 


Ala Ile Arg Thr Ile Tyr Pro Tyr Ile Lys Glu Asn Lys Phe Ser Thr 
                645                 650                 655     


Ala Cys Glu Leu Ala Gly Tyr Arg His Ser Lys Leu Ser Ile Ser Lys 
            660                 665                 670         


Glu Glu Asn Glu Asn Arg Thr Leu Lys Asp Thr Leu Asp Val Leu Lys 
        675                 680                 685             


Lys Asn Ser Leu Arg Gln Pro Val Val Glu Lys Ile Leu Asn Gln Met 
    690                 695                 700                 


Ile Asn Leu Ile Asn Glu Leu Ile Glu Lys His Ser Glu Lys Asp Glu 
705                 710                 715                 720 


Lys Gly Asn Ile Val Lys Tyr Phe Lys Phe Asp Glu Ile His Ile Glu 
                725                 730                 735     


Leu Ala Arg Glu Leu Lys Lys Asn Ala Glu Glu Arg Arg Glu Leu Asp 
            740                 745                 750         


Ser Lys Val Arg Glu Gly Lys Asp Arg Asn Glu Lys Ile Ile Thr Ile 
        755                 760                 765             


Leu Lys Ser Glu Phe Gly Leu Pro Tyr Pro Thr Lys Asn Asp Ile Thr 
    770                 775                 780                 


Arg Tyr Arg Leu Tyr Glu Glu Leu Lys Asp Asn Gly Tyr Lys Asp Leu 
785                 790                 795                 800 


Tyr Thr Asn Thr Tyr Ile Pro Arg Glu Lys Leu Phe Ser Lys Glu Ile 
                805                 810                 815     


Asp Ile Glu His Ile Ile Pro Lys Ser Arg Leu Phe Asn Asp Ser Phe 
            820                 825                 830         


Ser Asn Lys Thr Ile Cys Tyr Arg Gln Asp Asn Leu Asn Lys Gly Glu 
        835                 840                 845             


Arg Thr Ala Tyr Asp Tyr Ile Glu Gly Leu Gly Lys Thr Gln Leu Asp 
    850                 855                 860                 


Glu Tyr Leu Asn Arg Val Glu Asn Leu Tyr Thr Asn Lys Phe Ile Thr 
865                 870                 875                 880 


Lys Ala Lys Tyr Arg Asn Leu Leu Lys Lys Glu Ser Glu Ile Gly Asp 
                885                 890                 895     


Gly Phe Ile Glu Arg Asp Leu Arg Glu Thr Gln Tyr Ile Ala Lys Lys 
            900                 905                 910         


Ala Lys Glu Met Leu Leu Gln Ile Thr Arg His Val Val Ser Thr Thr 
        915                 920                 925             


Gly Ser Ile Thr Asp Arg Leu Arg Asp Asp Trp Glu Leu Val Asn Val 
    930                 935                 940                 


Met Arg Glu Leu Asn Leu Pro Lys Tyr Lys Val Leu Gly Leu Thr Glu 
945                 950                 955                 960 


Ile Gln Glu Arg Lys Asn Gly Gln Thr Val Glu Val Ile Thr Asp Trp 
                965                 970                 975     


Thr Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val 
            980                 985                 990         


Ala Phe Thr Lys His Ser His Ile  Gln Tyr Leu Asn Phe  Leu Asn Ala 
        995                 1000                 1005             


Arg Lys  Asp Glu Lys His Lys  Glu Tyr Ser Asn Ile  Met Gly Ile 
    1010                 1015                 1020             


Gln Gln  Leu Glu Thr Glu Lys  Thr Thr Asp Lys Asp  Gly Lys Asp 
    1025                 1030                 1035             


Arg Arg  Val Phe Lys Thr Pro  Ile Pro Asn Phe Arg  Gln Val Ala 
    1040                 1045                 1050             


Lys Glu  His Leu Glu Ser Ile  Leu Ile Ser His Lys  Thr Lys Asn 
    1055                 1060                 1065             


Lys Val  Val Thr Arg Ala Thr  Asn Lys Ile Ala Gly  Ser Lys Lys 
    1070                 1075                 1080             


Gln Gln  Glu Thr Leu Ser Pro  Arg Gly Gln Leu His  Lys Glu Thr 
    1085                 1090                 1095             


Val Tyr  Gly Lys Tyr Arg Arg  Tyr Val Gln Lys Glu  Glu Lys Ile 
    1100                 1105                 1110             


Gly Ser  Lys Phe Asp Leu Gln  Thr Ile Glu Arg Val  Ala Asn Pro 
    1115                 1120                 1125             


Leu Tyr  Lys Arg Leu Leu Thr  Lys Arg Leu Glu Glu  Asn Gly Gly 
    1130                 1135                 1140             


Asp Pro  Lys Lys Ala Phe Thr  Gly Lys Asn Ala Leu  Ala Lys Ser 
    1145                 1150                 1155             


Pro Ile  Tyr Leu Asp Glu Thr  Gln Thr Gln Gln Leu  Pro Glu Lys 
    1160                 1165                 1170             


Val Lys  Leu Val Trp Leu Glu  Thr Asp Phe Ser Ile  Arg Lys Asp 
    1175                 1180                 1185             


Ile Thr  Pro Asp Asn Phe Lys  Asp Glu Lys Ser Ile  Asp Lys Val 
    1190                 1195                 1200             


Leu Asp  Glu Gly Ile Lys Arg  Ile Leu Lys Asp Arg  Leu Lys Ser 
    1205                 1210                 1215             


Phe Asp  Gly Asp Ala Lys Arg  Ala Phe Thr Asp Leu  Glu Gln Asn 
    1220                 1225                 1230             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ala Ile  Lys Arg Val 
    1235                 1240                 1245             


Thr Ile  Ser Gly Val Ser Asn  Ala Glu Pro Leu His  Thr Lys Lys 
    1250                 1255                 1260             


Asp His  Leu Gly Asn Glu Ile  Phe Asp Asp Thr Gly  Lys Pro Ile 
    1265                 1270                 1275             


Pro Val  Asp Tyr Val Ser Thr  Gly Asn Asn His His  Ile Ala Ile 
    1280                 1285                 1290             


Tyr Arg  Asp Glu Asn Gly Asn  Leu Gln Glu Lys Ala  Val Ser Phe 
    1295                 1300                 1305             


Phe Glu  Ala Val Thr Arg Ala  Asn Gln Gly Leu Pro  Thr Val Asp 
    1310                 1315                 1320             


Lys Thr  Tyr Asn Gln His Leu  Gly Trp Gln Phe Leu  Phe Thr Met 
    1325                 1330                 1335             


Lys Gln  Asn Glu Met Phe Val  Phe Pro Asn Lys Glu  Thr Gly Phe 
    1340                 1345                 1350             


Asp Pro  Gln Glu Ile Asp Leu  Leu Asp Pro Lys Asn  Lys Lys Leu 
    1355                 1360                 1365             


Ile Ser  Pro Asn Leu Phe Arg  Val Gln Lys Ile Ser  Thr Lys Asn 
    1370                 1375                 1380             


Tyr Phe  Phe Arg His His Leu  Glu Thr Ser Val Glu  Thr His Asn 
    1385                 1390                 1395             


Ala Leu  Asn Gly Val Thr Tyr  Lys Ser Gln Leu Gly  Leu Asn Gly 
    1400                 1405                 1410             


Ile Thr  Asp Ile Ile Lys Val  Arg Ile Asn His Leu  Gly Asp Ile 
    1415                 1420                 1425             


Val His  Ile Gly Glu Tyr 
    1430                 


<210>  1243
<211>  1435
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1243

Met Leu Gly Cys Glu Arg Lys Arg Lys Arg Arg Glu Lys Glu Lys Met 
1               5                   10                  15      


Ala Lys Pro Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Val Val Thr Asp Asp Gln Asn Asn Ile Leu Arg Ile Lys 
        35                  40                  45              


Gly Lys Lys Ala Ile Gly Ala Arg Leu Phe Thr Glu Gly Lys Val Ala 
    50                  55                  60                  


Ala Glu Arg Arg Ser Phe Arg Thr Thr Arg Arg Arg Leu Ser Arg Arg 
65                  70                  75                  80  


Arg Trp Arg Ile Lys Met Leu Glu Glu Leu Phe Asp Glu Glu Ile Ala 
                85                  90                  95      


Lys Val Asp Pro Ser Phe Phe Ala Arg Leu His Glu Ser Trp Ile Ser 
            100                 105                 110         


Pro Lys Asp Lys Arg Lys Arg Tyr Ser Ala Ile Val Phe Pro Ser Pro 
        115                 120                 125             


Glu Glu Asp Lys Lys Phe His Glu Ser Tyr Pro Thr Ile Tyr His Leu 
    130                 135                 140                 


Arg Asp Lys Leu Met Lys Asp Asp Gln Lys His Asp Ile Arg Glu Ile 
145                 150                 155                 160 


Tyr Ile Ala Val His Gln Met Ile Lys Ala Arg Gly Asn Phe Leu His 
                165                 170                 175     


Asp Glu Ser Val Glu Thr Tyr Arg Ser Gly Met Ser Ser Leu Gly Gly 
            180                 185                 190         


Arg Ser Glu Arg Asn Ile Leu Ser Val Gln Thr Leu Glu Glu Leu Asn 
        195                 200                 205             


Asp Leu Phe Ala Glu Asn Glu Gly Thr Glu Glu Val Glu Leu Asn Val 
    210                 215                 220                 


Ala Ser Ala Glu Gln Ile Asn Asp Ile Leu Thr Gly Gly His Leu Asn 
225                 230                 235                 240 


Ala Asp Ser Gln Lys Glu Ile Ser Asn Leu Leu Leu Pro Ser Ser Phe 
                245                 250                 255     


Pro Ser Phe Asp Asp Lys Ala Lys Glu Lys Gln Val Lys Lys Leu Ile 
            260                 265                 270         


Asn Asn Val Ala Thr Asn Ile Ser Lys Ala Trp Leu Gly Tyr Lys Ala 
        275                 280                 285             


Asp Phe Ser Thr Ile Leu Asn Leu Ala Lys Val Asp Lys Asp Gln Lys 
    290                 295                 300                 


Lys Ile Phe Ala Phe Ala Leu Gln Gly Gly Asp Glu Glu Asp Lys Val 
305                 310                 315                 320 


Gln Glu Leu Glu Ser Leu Leu Glu Gln Ser Gln Thr Asp Ile Val Asp 
                325                 330                 335     


Arg Leu Ile Glu Ile Arg His Ala Ile Val Leu Ser Glu Ile Val Pro 
            340                 345                 350         


Val Gly Met Thr Leu Ser Glu Ala Met Ile Asp Lys Tyr Asp Gln His 
        355                 360                 365             


Lys Glu Asp Leu Ile Thr Leu Lys Ala Val Ile Arg Asn Thr Lys Asp 
    370                 375                 380                 


Lys Lys Lys Ala Ala Lys Leu Gln Ala Ile Tyr Asp Leu Tyr Val Lys 
385                 390                 395                 400 


Asn Arg His Ala Asp Leu Ala Lys Ala Met Lys Leu Thr Gly Ile Lys 
                405                 410                 415     


Lys Arg Ser Glu Leu Leu Asp Pro Glu Glu Leu Lys Lys Gly Ile Ser 
            420                 425                 430         


Ser Leu Leu Asp Asp Ser Pro Glu Ala Val Glu Ile Lys Gln Arg Leu 
        435                 440                 445             


Glu Glu Lys Thr Phe Leu Pro Leu Gln Arg Ser Asn Asn Asn Gly Val 
    450                 455                 460                 


Ile Pro Asn Gln Leu His Gln Val Glu Leu Asp Glu Ile Ile Lys Lys 
465                 470                 475                 480 


Gln Ser Lys Tyr Tyr Pro Phe Leu Ala Glu Lys Asn Pro Asp Glu Ser 
                485                 490                 495     


Glu Glu Ala Gln Lys Lys Ala Pro Thr Lys Leu Asp Ala Leu Leu Thr 
            500                 505                 510         


Phe Arg Val Pro Tyr Tyr Val Gly Pro Met Ile Thr Lys Glu Glu Gln 
        515                 520                 525             


Glu Ala Gln Asn Gly His Ser Phe Ala Trp Met Val Arg Arg Asp Pro 
    530                 535                 540                 


Gln Asp His Glu Ala Ile Thr Pro Trp Asn Phe Glu Lys Lys Val Asp 
545                 550                 555                 560 


Lys Met Ala Ser Ala Thr Gln Phe Ile Lys Arg Met Thr Thr Lys Asp 
                565                 570                 575     


Thr Tyr Leu Leu Gly Glu Asp Val Leu Pro Ala Ser Ser Leu Lys Tyr 
            580                 585                 590         


Gln Leu Phe Thr Val Leu Asn Glu Leu Asn Asn Leu Arg Val Asn Gly 
        595                 600                 605             


Lys Lys Leu Thr Ser Asp Glu Lys Glu Gln Val Ile Glu Gly Leu Phe 
    610                 615                 620                 


Lys Lys Gln Lys Thr Val Lys Ala Asp Lys Phe Val Lys Tyr Trp Gln 
625                 630                 635                 640 


Ala Lys His Ile Gly Ala Asp Ile Lys Val Lys Gly Leu Ser Asp Pro 
                645                 650                 655     


Ser Lys Phe Asn Ser Thr Met Ser Thr Tyr Ile Asp Phe Lys Lys Ile 
            660                 665                 670         


Phe Gly Asp Gln Leu Asn Asp Val Asn Arg Gln Asn Asp Phe Glu Lys 
        675                 680                 685             


Ile Ile Glu Tyr Ser Thr Ile Phe Glu Asp Arg Lys Ile Leu Glu Asp 
    690                 695                 700                 


Lys Leu Arg Val Asp Phe Ala Trp Leu Ser Glu Glu Gln Ile Lys Gln 
705                 710                 715                 720 


Leu Cys Arg Leu Arg Leu Gln Gly Trp Gly Arg Leu Ser Asp Lys Leu 
                725                 730                 735     


Leu Thr Arg Leu Thr Asp Ala Asp Gly Gln Asn Val Leu Glu Arg Leu 
            740                 745                 750         


Trp Asn Ser Asn Asp Asn Phe Val Gln Ile Val Ser Asp Pro Asn Ile 
        755                 760                 765             


Lys Ala Lys Ile Glu Gln Glu Asn Gln Lys Leu Leu Arg Asp Ser Gln 
    770                 775                 780                 


Ser Asp Asn Ala Val Glu Ser Ile Leu Asp Asp Ala Tyr Thr Ser Pro 
785                 790                 795                 800 


Gln Asn Lys Lys Ala Ile Arg Gln Val Met Arg Val Val His Asp Ile 
                805                 810                 815     


Val Lys Ala Cys Asp Gly Asn Val Pro Ala Lys Phe Ala Ile Glu Phe 
            820                 825                 830         


Ala Arg Ser Gln Glu Asp Asp Pro Lys Arg Thr Thr Ala Arg Val Asn 
        835                 840                 845             


Gln Met Gln Lys Ile Tyr Asp Gln Ile Ser Asp Glu Val Val Ser Gln 
    850                 855                 860                 


Gly Val Lys Glu Gln Leu Ser Gly Met Lys Ser Leu Lys Asp Arg Tyr 
865                 870                 875                 880 


Tyr Leu Tyr Phe Met Gln Gly Gly Arg Asp Ala Tyr Thr Gly Ala Lys 
                885                 890                 895     


Leu Asn Ile Asp Arg Leu Ser Asp Tyr Asp Val Asp His Ile Met Pro 
            900                 905                 910         


Gln Ser Phe Val Lys Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ala 
        915                 920                 925             


Arg Ala Val Asn Asn Gln Lys Ser Asp Lys Val Pro Ala Leu Leu Phe 
    930                 935                 940                 


Gly Asn Lys Val Val Ala Asp Leu Gly Ile Thr Val Arg Glu Met Trp 
945                 950                 955                 960 


Asp Lys Trp Gln Lys Leu Gly Met Ile Ser Lys Arg Lys Leu Ser Asn 
                965                 970                 975     


Leu Leu Thr Asp Ser Asp Ala Leu Thr Glu Tyr Arg Ala Gln Gly Phe 
            980                 985                 990         


Ile Arg Arg Gln Leu Val Glu Thr  Ser Gln Val Ile Lys  Leu Thr Ala 
        995                 1000                 1005             


Thr Ile  Leu Gln Ser Glu Phe  Pro Asp Ser Lys Ile  Ile Glu Val 
    1010                 1015                 1020             


Pro Ala  Lys Tyr Asn Ser Ile  Val Arg Lys Gln Phe  Asp Leu Tyr 
    1025                 1030                 1035             


Lys Ser  Arg Glu Val Asn Asp  Phe His His Ala Ile  Asp Ala Tyr 
    1040                 1045                 1050             


Leu Ser  Thr Ile Val Gly Asn  Tyr Leu Tyr Gln Val  Tyr Pro Asn 
    1055                 1060                 1065             


Leu Arg  Arg Met Phe Val Tyr  Gly Glu Phe Lys Lys  Phe Ser Ser 
    1070                 1075                 1080             


Asn Ala  Glu Glu Ser Ala His  Asp Val Ala Arg Arg  Val Lys Ser 
    1085                 1090                 1095             


Met Asn  Phe Leu Asp Asp Leu  Leu Arg Gly Thr His  Gly Asp Asn 
    1100                 1105                 1110             


Ile Tyr  Cys Arg Ser Thr Gly  Glu Ile Val Phe Asn  Arg Asn Asp 
    1115                 1120                 1125             


Ile Ile  Ser Lys Leu Lys Gln  Ala Tyr Ser Phe Lys  Gln Met Leu 
    1130                 1135                 1140             


Val Thr  Gln Glu Val Phe Thr  Lys Lys Ser Ala Leu  Phe Asn Gln 
    1145                 1150                 1155             


Thr Val  Tyr Pro Ser Pro Lys  Arg Asp Gly Ser Lys  Arg Ser Gly 
    1160                 1165                 1170             


Leu Ile  Pro Arg Lys Arg Gly  Met Asp Pro Glu Ile  Tyr Gly Gly 
    1175                 1180                 1185             


Tyr Ala  His Asn Gln Asp Ser  Tyr Phe Val Leu Ala  Lys Ile Glu 
    1190                 1195                 1200             


Lys Val  Lys Gly Tyr Thr Leu  Gln Ile Val Gly Val  Pro Ile Arg 
    1205                 1210                 1215             


Ser Leu  Glu Ile Ile Asn Lys  Ala Glu Asp Phe Ser  Thr Ala Leu 
    1220                 1225                 1230             


Leu Asp  Ile Ile Lys Ser Lys  Val Met Tyr Asn Glu  Lys Thr Gly 
    1235                 1240                 1245             


Gln Pro  Lys Arg Gly Ile Lys  Asn Val Glu Ile Leu  Val Asn Lys 
    1250                 1255                 1260             


Leu Pro  Tyr Arg Gln Pro Ile  Leu Glu Lys Asp Val  Ser Tyr Met 
    1265                 1270                 1275             


Leu Gly  Ser Ser Ala Tyr Lys  Tyr Asn Ile Lys Gln  Ile Thr Leu 
    1280                 1285                 1290             


Ser Arg  Gln Ser Met Lys Tyr  Ile Leu Asp Tyr Ile  Asp Asp Pro 
    1295                 1300                 1305             


Asn Phe  Thr Lys His Glu Met  Thr Met Ile Asp Glu  Gln Asn Glu 
    1310                 1315                 1320             


Arg Glu  Cys Leu Leu Ser Val  Tyr Asp Glu Ile Leu  Glu Lys Val 
    1325                 1330                 1335             


Asp Lys  Tyr Leu Pro Leu Phe  Asp Gly Asn Lys Phe  Arg Glu Lys 
    1340                 1345                 1350             


Leu His  Ser Ala Arg Lys Glu  Phe Val Ser Leu Ser  Val Asp Asp 
    1355                 1360                 1365             


Glu Ala  Ser Ala Ile Glu Lys  Ile Leu Arg Gly Leu  His Ala Asn 
    1370                 1375                 1380             


Ser Glu  Met Pro Ser Leu Glu  Asn Leu Lys Ile Lys  Pro Ala Leu 
    1385                 1390                 1395             


Gly Phe  Phe Gln Met Lys Asn  Gly Ile Thr Leu Ser  Asn Asp Ala 
    1400                 1405                 1410             


Val Leu  Leu Tyr Gln Ser Pro  Thr Gly Leu Phe Glu  Arg Arg Val 
    1415                 1420                 1425             


Lys Val  Ser Asp Leu Leu Lys  
    1430                 1435 


<210>  1244
<211>  1435
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1244

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Phe 
1               5                   10                  15      


Ala His Ile Val Glu Asp Glu Asn Lys Glu Lys Ser Glu Ile Lys Glu 
            20                  25                  30          


Leu Gly Val Arg Ile Val Pro Leu Thr Thr Asp Glu Gln Ser Asp Phe 
        35                  40                  45              


Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asn Arg Thr Leu Lys His 
    50                  55                  60                  


Gly Ala Arg Leu Asn Leu Asp Arg Tyr Gln Gln Arg Arg Lys Tyr Leu 
65                  70                  75                  80  


Ile Asp Leu Leu Gln Lys Ala Asn Leu Ile Thr Ser Ser Ser Ile Leu 
                85                  90                  95      


Ala Glu Asn Gly Lys Asn Thr Thr His Ser Thr Trp Gln Leu Arg Ala 
            100                 105                 110         


Lys Ala Val Thr Glu Arg Ile Glu Lys Glu Glu Phe Ala Arg Val Leu 
        115                 120                 125             


Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala Lys 
    130                 135                 140                 


Thr Glu Asp Glu Gly Gln Ala Ile Asp Gly Met Ala Ile Ala Lys Arg 
145                 150                 155                 160 


Leu Tyr Asp Glu Asn Leu Thr Thr Gly Gln Leu Ser Leu Gln Leu Leu 
                165                 170                 175     


Gln Gln Asn Lys Lys Leu Leu Pro Asp Phe Tyr Arg Ser Asp Leu Gln 
            180                 185                 190         


Lys Glu Phe Asp Leu Val Trp Asn Phe Gln Lys Gln Phe Tyr Pro Asp 
        195                 200                 205             


Ile Leu Thr Asp Ser Phe Tyr Lys Glu Leu Gln Gly Lys Gly Lys Asp 
    210                 215                 220                 


Ala Thr Ser Lys Ala Phe Ser Lys Arg Tyr His Phe Asp Thr Ala Glu 
225                 230                 235                 240 


Asn Lys Gly Ser Lys Glu Ser Val Arg Leu Gln Ala Tyr Gln Trp Arg 
                245                 250                 255     


Val Glu Ala Ile Ser Lys Gln Leu Ser Lys Glu Glu Val Ala Tyr Val 
            260                 265                 270         


Leu Thr Glu Ile Asn Asn Asn Leu Asn Asn Ala Ser Gly Tyr Leu Gly 
        275                 280                 285             


Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Arg Gln Thr Val 
    290                 295                 300                 


Gly Gln Tyr Leu Tyr Ala Gln Leu Gln Glu Asn Arg His Asn Ser Leu 
305                 310                 315                 320 


Lys Asn Lys Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Arg 
                325                 330                 335     


Ile Trp Glu Thr Gln Ala Ser Phe His Lys Glu Leu Thr Glu Glu Leu 
            340                 345                 350         


Lys Lys Gln Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Pro Lys 
        355                 360                 365             


Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu Phe Glu Ser Lys Glu Ile 
    370                 375                 380                 


Glu Ile Glu Lys Asp Gly Lys Thr Ile Thr Lys Thr Ile Gly Ala Arg 
385                 390                 395                 400 


Val Val Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln 
                405                 410                 415     


Ile Leu Asn Asn Val Ile Cys Lys Arg Lys Gly Ile Arg Lys Lys Lys 
            420                 425                 430         


Val Ser Ala Lys Thr Thr Gln Leu Asp Leu Leu Asn Glu Ser Ser Gln 
        435                 440                 445             


Thr Ile Phe Ser Leu Asp Met Glu Cys Lys Gln Leu Leu Phe Glu Glu 
    450                 455                 460                 


Leu Asn Leu Lys Gly Asp Leu Lys Ser Asp Lys Val Leu Lys Leu Leu 
465                 470                 475                 480 


Gly Tyr Ser Pro Gln Glu Trp Glu Ile Asn Tyr Asn Gln Leu Glu Gly 
                485                 490                 495     


Asn Arg Thr Gln Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Val Glu 
            500                 505                 510         


Met Glu Ala His Asp Val Lys Asp Ile Leu Gln Ile Lys Ser Ala Lys 
        515                 520                 525             


Asp Asp Trp Ser Leu Asp Glu Ser Pro Leu Ser Ala Ser Glu Ile Arg 
    530                 535                 540                 


Glu Ser Val Lys Ala Ile Phe Gln Ala Leu Gly Ile Cys Thr Lys Ile 
545                 550                 555                 560 


Leu Tyr Phe Asp Pro Leu Leu Pro Val Lys Glu Phe Glu Lys Gln Asp 
                565                 570                 575     


Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asp Ser 
            580                 585                 590         


Thr Ser Gly Asn Glu Thr Leu Tyr Arg Ile Leu Glu Lys Lys Tyr Ala 
        595                 600                 605             


Phe Lys Arg Glu His Val Arg Ile Leu Ala Asn Val Ala Leu Gln Asp 
    610                 615                 620                 


Asp Tyr Gly Ser Leu Ser Thr Lys Ala Ile Arg Lys Ile Tyr Pro Asn 
625                 630                 635                 640 


Ile Lys Glu Asn Gln Tyr Ser Ile Ala Cys Glu Met Ala Gly Tyr Lys 
                645                 650                 655     


His Ser Lys Leu Ser Leu Thr Thr Glu Glu Leu Glu Ala Arg Glu Leu 
            660                 665                 670         


Lys Asn Ile Ile Pro Leu Leu Lys Lys Asn Ala Leu Arg Asn Pro Val 
        675                 680                 685             


Val Glu Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Ala Leu Ile 
    690                 695                 700                 


Glu Lys Asn Ser Glu Arg Asp Ala Glu Gly Lys Ile Thr Lys Tyr Phe 
705                 710                 715                 720 


His Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn 
                725                 730                 735     


Ala Gln Lys Arg Tyr Glu Met Thr Gln Asn Ile Asn Lys Ala Lys Leu 
            740                 745                 750         


Glu His Gln Lys Ile Ser Glu Ile Leu Gln Lys Gln Phe Gly Ile Lys 
        755                 760                 765             


Asn Pro Thr Lys Ser Asp Ile Ile Arg Tyr Arg Leu Tyr Gln Glu Leu 
    770                 775                 780                 


Glu His Asn Gly Tyr Lys Glu Leu Tyr Thr Asn Ala Tyr Ile Ala Pro 
785                 790                 795                 800 


Asp Val Leu Phe Ser Lys Asn Ile Asp Ile Glu His Ile Val Pro Lys 
                805                 810                 815     


Ala Arg Val Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Ala Phe Arg 
            820                 825                 830         


Glu Phe Asn Ile Lys Lys Gly Glu Ser Thr Ala Phe Asp Tyr Ile Thr 
        835                 840                 845             


Ser Leu Asn Ser Glu Glu Glu Leu Asn Gln Tyr Leu Thr Arg Val Glu 
    850                 855                 860                 


Asn Ala Tyr Lys Thr Lys Ser Ile Ser Pro Thr Lys Tyr Lys Asn Leu 
865                 870                 875                 880 


Leu Lys Lys Ala Ser Glu Ile Gly Asp Asp Phe Ile Asn Arg Asp Leu 
                885                 890                 895     


Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Phe Gln 
            900                 905                 910         


Val Thr Lys Asn Val Leu Ser Thr Ser Gly Ser Ile Thr Asp Arg Leu 
        915                 920                 925             


Arg Glu Asp Trp Gly Leu Val Asp Val Met Lys Glu Leu Asn Met Pro 
    930                 935                 940                 


Lys Tyr Gln Ser Leu Gly Leu Thr Glu Val Glu Glu Arg Lys Asp Gly 
945                 950                 955                 960 


Asn Lys Val Thr Val Ile Lys Asn Trp Thr Lys Arg Asn Asp His Arg 
                965                 970                 975     


His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Pro Ser Tyr 
            980                 985                 990         


Ile Gln Tyr Leu Asn His Leu Asn  Ala Arg Lys Asp Glu  Asn Asn Lys 
        995                 1000                 1005             


Asn Tyr  Ser Val Ile Leu Ala  Ile Glu Glu Lys Glu  Thr Ile Lys 
    1010                 1015                 1020             


Val Arg  Thr Asn Asn Gly Lys  Asn Lys Arg Val Phe  Ile Glu Pro 
    1025                 1030                 1035             


Ile Pro  Asn Phe Arg Gln Val  Ala Lys Lys His Leu  Glu Glu Ile 
    1040                 1045                 1050             


Phe Ile  Ser His Lys Thr Lys  Asn Lys Val Val Thr  Lys Asn Val 
    1055                 1060                 1065             


Asn Lys  Pro Ala Gly Thr Asp  Lys Gln Gln Ile Thr  Leu Thr Pro 
    1070                 1075                 1080             


Arg Gly  Gln Leu His Lys Glu  Thr Ile Tyr Gly Lys  Tyr Gln Tyr 
    1085                 1090                 1095             


Tyr Val  Asn Lys Glu Glu Lys  Ile Gly Val Lys Phe  Asp Lys Glu 
    1100                 1105                 1110             


Thr Ile  Ala Lys Val Ser Asn  Pro Leu Tyr Arg Glu  Ala Leu Leu 
    1115                 1120                 1125             


Lys Arg  Leu Gln Ala Asn Asp  Asn Asp Pro Lys Lys  Ala Phe Thr 
    1130                 1135                 1140             


Gly Lys  Asn Ala Leu Ser Lys  Lys Pro Ile Tyr Leu  Asp Glu Ser 
    1145                 1150                 1155             


Lys Thr  Lys Thr Leu Pro Glu  Lys Val Asn Leu Thr  Tyr Leu Glu 
    1160                 1165                 1170             


Glu Asp  Phe Ser Ile Arg Lys  Asp Ile Ser Pro Asp  Asn Phe Lys 
    1175                 1180                 1185             


Asp Leu  Lys Ser Ile Glu Lys  Val Ile Asp Gln Gly  Val Lys Arg 
    1190                 1195                 1200             


Ile Leu  Ile Lys Arg Leu Gln  Ala Tyr Asp Asn Asp  Pro Lys Lys 
    1205                 1210                 1215             


Ala Phe  Val Asp Leu Glu Lys  Asn Pro Ile Trp Leu  Asn Lys Glu 
    1220                 1225                 1230             


Lys Gly  Ile Ala Ile Lys Arg  Val Thr Ile Ser Gly  Val Asn Asn 
    1235                 1240                 1245             


Ala Gln  Pro Leu His Ile Gly  Lys Asp His Leu Gly  Lys Thr Thr 
    1250                 1255                 1260             


Leu Asn  Lys Glu Gly Lys Glu  Ile Pro Val Asp Tyr  Val Ser Thr 
    1265                 1270                 1275             


Gly Asn  Asn His His Val Ala  Ile Tyr Arg Asp Lys  Glu Gly Asn 
    1280                 1285                 1290             


Leu Gln  Glu Gln Ile Val Ser  Phe Phe Asp Ala Val  Val Arg Ala 
    1295                 1300                 1305             


Gln Gln  Gly Leu Pro Ile Ile  Asp Lys Thr Tyr Lys  Gln Ala Glu 
    1310                 1315                 1320             


Gly Trp  Gln Phe Met Phe Thr  Met Lys Gln Asn Glu  Met Phe Val 
    1325                 1330                 1335             


Phe Pro  Asn Glu Thr Thr Gly  Phe Asn Pro Ala Glu  Ile Asp Leu 
    1340                 1345                 1350             


Leu Asp  Pro Lys Asn Lys Lys  Leu Ile Ser Pro Asn  Leu Phe Arg 
    1355                 1360                 1365             


Val Gln  Thr Ile Ser Val Val  Lys Tyr Gly Asn Ser  Val Val Arg 
    1370                 1375                 1380             


Asp Phe  Lys Phe Arg His His  Leu Glu Thr Thr Leu  Gln Asp Asp 
    1385                 1390                 1395             


Lys Ile  Leu Gln Gly Ile Thr  Phe Gln Gln Ile Lys  Ser Leu Glu 
    1400                 1405                 1410             


Pro Leu  Arg Asn Ile Val Lys  Val Arg Ile Asn His  Leu Gly Asp 
    1415                 1420                 1425             


Ile Val  Ser Ile Gly Glu Tyr  
    1430                 1435 


<210>  1245
<211>  1435
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1245

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Phe 
1               5                   10                  15      


Ala His Ile Val Glu Asp Glu Asn Lys Glu Lys Ser Glu Ile Lys Glu 
            20                  25                  30          


Leu Gly Val Arg Ile Val Ser Leu Ala Thr Asp Glu Gln Ser Asp Phe 
        35                  40                  45              


Glu Lys Gly Lys Ser Ile Ser Thr Asn Ala Asn Arg Thr Leu Lys His 
    50                  55                  60                  


Gly Ala Arg Leu Asn Leu Asp Arg Tyr Gln Gln Arg Arg Lys Phe Leu 
65                  70                  75                  80  


Ile Asp Leu Leu Gln Lys Ala Asn Leu Ile Thr Ser Ala Ser Ile Leu 
                85                  90                  95      


Ala Glu Asn Gly Lys Asn Thr Thr His Ser Thr Trp Gln Leu Arg Ala 
            100                 105                 110         


Lys Ala Val Thr Glu Arg Ile Glu Lys Glu Glu Phe Ala Arg Val Leu 
        115                 120                 125             


Leu Ala Ile Asn Lys Lys Arg Gly Tyr Lys Asn Ser Arg Lys Thr Lys 
    130                 135                 140                 


Thr Glu Asp Glu Gly Gln Ala Ile Asp Gly Met Ala Ile Ala Lys Cys 
145                 150                 155                 160 


Leu Tyr Asp Glu Asn Leu Thr Pro Gly Gln Leu Ser Leu Gln Leu Leu 
                165                 170                 175     


Gln Gln Asn Lys Lys Leu Leu Pro Asp Phe Tyr Arg Ser Asp Leu Gln 
            180                 185                 190         


Lys Glu Phe Asp Leu Val Trp Asn Leu Gln Lys Gln Phe Tyr Pro Asp 
        195                 200                 205             


Ile Leu Thr Asp Ser Phe Tyr Lys Glu Leu Gln Gly Lys Gly Lys Asp 
    210                 215                 220                 


Ala Thr Ser Lys Ala Phe Ser Lys Arg Tyr His Phe Asp Thr Ala Glu 
225                 230                 235                 240 


Asn Lys Gly Ser Lys Glu Ser Val Arg Leu Gln Ala Tyr Gln Trp Arg 
                245                 250                 255     


Ala Glu Ala Ile Ser Lys Gln Leu Ser Lys Glu Glu Val Ala Tyr Val 
            260                 265                 270         


Leu Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr Leu Gly 
        275                 280                 285             


Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Arg Gln Thr Val 
    290                 295                 300                 


Gly Gln Tyr Leu Tyr Ala Gln Leu Gln Glu Asn Arg His Asn Ser Leu 
305                 310                 315                 320 


Lys Asn Lys Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Arg 
                325                 330                 335     


Ile Trp Glu Thr Gln Ala Ser Phe His Lys Glu Leu Thr Asp Glu Leu 
            340                 345                 350         


Lys Lys Gln Ile Arg Asp Val Val Ile Phe Tyr Gln Arg Lys Leu Lys 
        355                 360                 365             


Ser Gln Lys Gly Leu Val Ser Phe Cys Glu Phe Glu Ser Lys Glu Ile 
    370                 375                 380                 


Glu Ile Glu Lys Asp Gly Lys Ala Ile Thr Lys Ser Ile Gly Ala Arg 
385                 390                 395                 400 


Val Val Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys Ile Trp Gln 
                405                 410                 415     


Ile Leu Asn Asn Val Thr Cys Lys Arg Lys Gly Ile Arg Lys Lys Lys 
            420                 425                 430         


Ser Ser Ala Lys Ser Thr Glu Leu Asp Leu Phe Asn Glu Pro Ser Gln 
        435                 440                 445             


Ser Ile Phe Ser Leu Asp Met Glu Cys Lys Gln Leu Leu Phe Asp Glu 
    450                 455                 460                 


Leu Asn Leu Lys Gly Asp Leu Lys Ser Asp Lys Val Leu Lys Leu Leu 
465                 470                 475                 480 


Gly Tyr Ser Pro Gln Glu Trp Glu Ile Asn Tyr Asn Gln Leu Glu Gly 
                485                 490                 495     


Asn Arg Thr Gln Lys Ser Leu Tyr Glu Ala Tyr Leu Lys Ile Ile Glu 
            500                 505                 510         


Ile Glu Gly Tyr Glu Val Lys Asp Ile Leu Gln Ile Lys Ser Thr Lys 
        515                 520                 525             


Asp Asp Trp Ser Leu Asp Glu Ser Pro Leu Ser Ala Ser Glu Ile Arg 
    530                 535                 540                 


Glu Arg Val Lys Val Ile Phe Gln Thr Leu Gly Ile Cys Thr Lys Ile 
545                 550                 555                 560 


Leu Tyr Phe Asp Pro Leu Leu Pro Val Lys Glu Phe Glu Gln Gln Tyr 
                565                 570                 575     


Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asp Ser 
            580                 585                 590         


Thr Ser Gly Asn Glu Thr Leu Tyr Arg Ile Leu Glu Lys Lys Tyr Ala 
        595                 600                 605             


Phe Lys Arg Glu His Ala Thr Ile Leu Ala Asn Val Ala Leu Gln Asp 
    610                 615                 620                 


Asp Tyr Gly Ser Leu Ser Thr Lys Ala Ile Arg Lys Ile Tyr Pro Tyr 
625                 630                 635                 640 


Ile Lys Glu Asn Gln Tyr Ser Thr Ala Cys Glu Leu Ala Gly Tyr Lys 
                645                 650                 655     


His Ser Lys Leu Ser Leu Thr Thr Glu Glu Leu Glu Ala Arg Glu Leu 
            660                 665                 670         


Lys Asn Ile Ile Pro Leu Leu Lys Lys Asn Ala Leu Arg Asn Pro Val 
        675                 680                 685             


Val Glu Lys Ile Leu Asn Gln Met Ile Asn Val Val Asn Ala Leu Ile 
    690                 695                 700                 


Glu Lys Asn Ser Glu Arg Asp Ala Glu Gly Lys Ile Thr Lys Tyr Phe 
705                 710                 715                 720 


His Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Lys Asn 
                725                 730                 735     


Ala Gln Glu Arg Tyr Glu Met Thr Gln Asn Ile Asn Lys Ala Lys Leu 
            740                 745                 750         


Glu His Gln Lys Ile Ser Glu Lys Leu Gln Lys Gln Phe Gly Ile Lys 
        755                 760                 765             


Asn Pro Thr Lys Ser Asp Ile Ile Arg Tyr Arg Leu Tyr Gln Glu Leu 
    770                 775                 780                 


Glu His Asn Gly Tyr Lys Glu Leu Tyr Thr Asn Thr Tyr Ile Ala Pro 
785                 790                 795                 800 


Asp Val Leu Phe Ser Lys Asn Ile Asp Ile Glu His Ile Val Pro Lys 
                805                 810                 815     


Ala Arg Val Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Ala Phe Lys 
            820                 825                 830         


Glu Phe Asn Ile Lys Lys Gly Glu Ser Thr Ala Phe Asp Tyr Ile Thr 
        835                 840                 845             


Ser Leu Asn Cys Glu Gly Glu Leu Asn Gln Tyr Leu Thr Arg Val Glu 
    850                 855                 860                 


Asn Ala Tyr Lys Thr Lys Ser Ile Ser Leu Thr Lys Tyr Lys Asn Leu 
865                 870                 875                 880 


Leu Lys Lys Ala Ser Glu Ile Gly Asp Gly Phe Ile Asn Arg Asp Leu 
                885                 890                 895     


Arg Asp Thr Gln Tyr Ile Ala Lys Lys Ala Lys Glu Ile Leu Phe Gln 
            900                 905                 910         


Val Thr Lys Asn Val Leu Ser Thr Ser Gly Ser Ile Thr Asp Arg Leu 
        915                 920                 925             


Arg Glu Asp Trp Gly Leu Val Asp Val Met Lys Glu Leu Asn Met Pro 
    930                 935                 940                 


Lys Tyr Gln Ser Leu Gly Leu Thr Glu Val Glu Glu Arg Lys Asp Gly 
945                 950                 955                 960 


Asn Lys Val Thr Val Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg 
                965                 970                 975     


His His Ala Met Asp Ala Leu Thr Val Ala Phe Thr Lys Pro Ser Tyr 
            980                 985                 990         


Ile Gln Tyr Leu Asn His Leu Asn  Ala Arg Lys Asp Glu  Asn Asn Lys 
        995                 1000                 1005             


Asn Tyr  Ser Val Ile Leu Ala  Ile Glu Lys Lys Glu  Thr Ile Lys 
    1010                 1015                 1020             


Val Pro  Ile Asn Asn Gly Lys  Asn Lys Arg Val Phe  Ile Glu Pro 
    1025                 1030                 1035             


Ile Leu  Asn Phe Arg Gln Val  Ala Lys Lys His Leu  Glu Glu Ile 
    1040                 1045                 1050             


Phe Ile  Ser His Lys Ala Lys  Asn Lys Val Val Thr  Lys Asn Ile 
    1055                 1060                 1065             


Asn Lys  Pro Ala Gly Thr Asp  Lys Gln Gln Ile Thr  Leu Thr Pro 
    1070                 1075                 1080             


Arg Gly  Gln Leu His Lys Glu  Thr Ile Tyr Gly Lys  Tyr Gln Tyr 
    1085                 1090                 1095             


Tyr Val  Asn Lys Glu Glu Lys  Val Gly Val Lys Phe  Asp Glu Arg 
    1100                 1105                 1110             


Thr Ile  Thr Lys Val Ser Asn  Pro Leu Tyr Arg Glu  Ala Leu Leu 
    1115                 1120                 1125             


Lys Arg  Leu Gln Ala Asn Asp  Asn Asp Pro Lys Lys  Ala Phe Ala 
    1130                 1135                 1140             


Gly Lys  Asn Ala Leu Ser Lys  Asn Pro Ile Tyr Leu  Asp Glu Ala 
    1145                 1150                 1155             


Lys Thr  Lys Thr Leu Pro Glu  Lys Val Asn Leu Thr  Tyr Leu Glu 
    1160                 1165                 1170             


Glu Asp  Phe Ser Ile Arg Lys  Asp Ile Ser Pro Asp  Asn Phe Lys 
    1175                 1180                 1185             


Asp Leu  Lys Ser Ile Glu Lys  Val Ile Asp Gln Gly  Val Lys Arg 
    1190                 1195                 1200             


Ile Leu  Ile Lys Arg Leu Gln  Ala Tyr Asp Asn Asp  Pro Lys Lys 
    1205                 1210                 1215             


Ala Phe  Val Asp Leu Glu Lys  Asn Pro Ile Trp Leu  Asn Lys Glu 
    1220                 1225                 1230             


Lys Gly  Ile Ala Ile Lys Arg  Val Thr Ile Ser Gly  Val Ser Asn 
    1235                 1240                 1245             


Ala Gln  Pro Leu His Ile Gly  Lys Asp His Leu Gly  Lys Thr Thr 
    1250                 1255                 1260             


Leu Asn  Lys Glu Gly Lys Glu  Val Pro Val Asp Tyr  Val Ser Thr 
    1265                 1270                 1275             


Gly Asn  Asn His His Val Ala  Ile Tyr Arg Asp Lys  Glu Gly Asn 
    1280                 1285                 1290             


Leu Gln  Glu Gln Ile Val Ser  Phe Phe Glu Ala Val  Val Arg Ala 
    1295                 1300                 1305             


Gln Gln  Gly Leu Pro Ile Ile  Asp Lys Asn Tyr Lys  Gln Ala Glu 
    1310                 1315                 1320             


Gly Trp  Gln Phe Met Phe Thr  Met Lys Gln Asn Glu  Met Phe Val 
    1325                 1330                 1335             


Phe Pro  Asn Glu Ala Thr Gly  Phe Asn Pro Ala Glu  Ile Asp Leu 
    1340                 1345                 1350             


Leu Asp  Pro Lys Asn Lys Lys  Leu Ile Ser Pro Asn  Leu Phe Arg 
    1355                 1360                 1365             


Val Gln  Thr Ile Ser Ile Val  Lys Tyr Gly Asn Ser  Val Val Arg 
    1370                 1375                 1380             


Asp Phe  Lys Phe Arg His His  Leu Glu Thr Thr Leu  Gln Asp Asp 
    1385                 1390                 1395             


Lys Ile  Leu Gln Gly Ile Thr  Phe Gln Gln Ile Lys  Ser Leu Glu 
    1400                 1405                 1410             


Pro Leu  Arg Asn Ile Val Lys  Val Arg Ile Asn His  Leu Gly Asp 
    1415                 1420                 1425             


Ile Val  Asn Ile Gly Glu Tyr  
    1430                 1435 


<210>  1246
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1246

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Lys Asp Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Thr 
65                  70                  75                  80  


Leu Thr Glu Val Leu Lys Glu His Lys Leu Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Thr Glu Glu Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Val Glu Glu Gly Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Glu Leu Tyr Asn Asn Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asp Ala Gly Lys Lys Phe Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Val Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Tyr Phe Val Trp Lys Glu Asn 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Lys Glu Lys Gly Lys Thr Glu Gln Gln Glu Arg 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Arg Glu Asn Leu Gln Trp Arg Val Asn Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Val Phe Gln Glu Met Asn Thr Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Tyr Gln Met Glu Met Leu 
                325                 330                 335     


Asp Lys Asn Pro Asn Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Met Leu Trp Glu Lys Gln Ala Val Tyr 
        355                 360                 365             


His Lys Glu Leu Thr Glu Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Asp Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Val Gly Asn Arg Val Ile Ser Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Val Val Gly Lys Lys Arg Lys Arg Arg Lys Leu Lys Glu Asn Tyr Ser 
    450                 455                 460                 


Ala Leu Phe Glu Glu Leu Asn Asp Ala Glu Gln Leu Glu Leu Asn Gly 
465                 470                 475                 480 


Ser Arg Arg Leu Cys Gln Glu Glu Lys Glu Leu Leu Ala Gln Glu Leu 
                485                 490                 495     


Phe Val Arg Asp Lys Met Thr Lys Ser Glu Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asp Asn Pro Gln Glu Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn 
        515                 520                 525             


Lys Thr Gly Tyr Ala Leu Phe Gln Ala Tyr Ser Lys Met Ile Glu Met 
    530                 535                 540                 


Ser Gly His Glu Pro Val Asp Phe Lys Lys Pro Val Glu Lys Val Val 
545                 550                 555                 560 


Glu Tyr Ile Lys Ala Val Phe Asp Leu Leu Asn Trp Asn Thr Asp Ile 
                565                 570                 575     


Leu Gly Phe Asn Ser Asn Glu Glu Leu Asp Asn Gln Ser Tyr Tyr Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Arg Leu Ile Gln Lys Met Thr Glu Leu Tyr Gly Phe Glu Lys 
    610                 615                 620                 


Glu Tyr Ala Thr Ile Leu Ala Asn Val Ser Phe Gln Asp Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile His Lys Ile Leu Pro His Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Val Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Glu Ser Ser Leu Thr Arg Glu Glu Ile Ala Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Arg Leu Met Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Val Ile Ile Asp Ile 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Ser Ile Ala Gln Thr 
            740                 745                 750         


Thr Lys Ala His Glu Glu Tyr Lys Thr Leu Leu Gln Thr Glu Phe Gly 
        755                 760                 765             


Leu Thr Asn Val Ser Arg Thr Asp Ile Leu Arg Tyr Lys Leu Tyr Lys 
    770                 775                 780                 


Glu Leu Glu Ser Cys Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Ser Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Ala Arg Ser Val Asn Ile Glu Lys Gly Asn Asn Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Phe Gly Glu Ser Gly Ala Asp Asn Ser Leu Glu Gln 
    850                 855                 860                 


Tyr Leu Asn Asn Ile Glu Asp Leu Phe Lys Ser Gly Lys Ile Ser Lys 
865                 870                 875                 880 


Thr Lys Tyr Asn Lys Leu Lys Met Ala Glu Gln Asp Ile Pro Asp Gly 
                885                 890                 895     


Phe Ile Glu Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala 
            900                 905                 910         


Leu Ser Met Leu Asn Glu Ile Ser His Arg Val Val Ala Thr Ser Gly 
        915                 920                 925             


Ser Val Thr Asp Lys Leu Arg Glu Asp Trp Gln Leu Ile Asp Val Met 
    930                 935                 940                 


Lys Glu Leu Asn Trp Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr 
945                 950                 955                 960 


Phe Glu Asp Arg Asp Gly Arg Gln Ile Gly Arg Ile Lys Asp Trp Thr 
                965                 970                 975     


Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala 
            980                 985                 990         


Phe Thr Lys Asp Val Phe Ile Gln  Tyr Phe Asn Asn Lys  Asn Ala Ser 
        995                 1000                 1005             


Leu Asp  Pro Asn Ala Asn Glu  His Ala Ile Lys Asn  Lys Tyr Phe 
    1010                 1015                 1020             


Gln Asn  Gly Arg Ala Ile Ala  Pro Met Pro Leu Arg  Glu Phe Arg 
    1025                 1030                 1035             


Ala Glu  Ala Lys Lys His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Ile Thr  Gly Asn Ile Asn Lys  Thr Arg Lys 
    1055                 1060                 1065             


Lys Gly  Gly Val Asn Lys Asn  Met Gln Gln Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Gly Lys Gln  Tyr Leu Thr 
    1085                 1090                 1095             


Lys Glu  Glu Lys Val Asn Ala  Ser Phe Asp Met Arg  Lys Ile Gly 
    1100                 1105                 1110             


Thr Val  Ser Lys Ser Ala Tyr  Arg Asp Ala Leu Leu  Lys Arg Leu 
    1115                 1120                 1125             


Tyr Glu  Asn Asp Asn Asp Pro  Lys Lys Ala Phe Ala  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Asp Lys Gln Pro Ile  Trp Leu Asp Lys Glu  Gln Met Arg 
    1145                 1150                 1155             


Lys Val  Pro Glu Lys Val Lys  Ile Val Thr Leu Glu  Ala Ile Tyr 
    1160                 1165                 1170             


Thr Ile  Arg Lys Glu Ile Ser  Pro Asp Leu Lys Val  Asp Lys Val 
    1175                 1180                 1185             


Ile Asp  Val Gly Val Arg Lys  Ile Leu Ile Asp Arg  Leu Asn Glu 
    1190                 1195                 1200             


Tyr Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Glu Asn 
    1205                 1210                 1215             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1220                 1225                 1230             


Thr Ile  Ser Gly Ile Ser Asn  Ala Gln Ser Leu His  Val Lys Lys 
    1235                 1240                 1245             


Asp Lys  Asp Gly Lys Pro Ile  Leu Asp Glu Asn Gly  Arg Asn Ile 
    1250                 1255                 1260             


Pro Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Val 
    1265                 1270                 1275             


Tyr Tyr  Arg Pro Val Ile Asp  Lys Arg Gly Gln Leu  Val Val Asp 
    1280                 1285                 1290             


Glu Ala  Gly Asn Pro Lys Tyr  Glu Leu Glu Glu Val  Val Val Ser 
    1295                 1300                 1305             


Phe Phe  Glu Ala Val Thr Arg  Ala Asn Leu Gly Gln  Pro Ile Ile 
    1310                 1315                 1320             


Asp Lys  Asp Tyr Lys Thr Thr  Glu Gly Trp Gln Phe  Leu Phe Ser 
    1325                 1330                 1335             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Glu  Lys Thr Gly 
    1340                 1345                 1350             


Phe Asn  Pro Lys Glu Ile Asp  Leu Leu Asp Val Glu  Asn Tyr Gly 
    1355                 1360                 1365             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Phe  Ser Leu Lys 
    1370                 1375                 1380             


Asn Tyr  Val Phe Arg His His  Leu Glu Thr Thr Ile  Lys Asp Thr 
    1385                 1390                 1395             


Ser Ser  Ile Leu Arg Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser 
    1400                 1405                 1410             


Lys Gly  Leu Asp Thr Ile Val  Lys Val Arg Val Asn  His Ile Gly 
    1415                 1420                 1425             


Gln Ile  Val Ser Val Gly Glu  Tyr 
    1430                 1435     


<210>  1247
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1247

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Lys Asp Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Thr 
65                  70                  75                  80  


Leu Thr Glu Val Leu Lys Glu His Lys Leu Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Thr Glu Glu Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Val Glu Glu Gly Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Glu Leu Tyr Asn Asn Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asp Ala Gly Lys Lys Phe Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Val Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Tyr Phe Val Trp Lys Glu Asn 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Lys Glu Lys Gly Lys Thr Glu Gln Gln Glu Arg 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Arg Glu Asn Leu Gln Trp Arg Val Asn Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Val Phe Gln Glu Met Asn Thr Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Tyr Gln Met Glu Met Leu 
                325                 330                 335     


Asp Lys Asn Pro Asn Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Met Leu Trp Glu Lys Gln Ala Val Tyr 
        355                 360                 365             


His Lys Glu Leu Thr Glu Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Asp Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Val Gly Asn Arg Val Ile Ser Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Val Val Gly Lys Lys Arg Lys Arg Arg Lys Leu Lys Glu Asn Tyr Ser 
    450                 455                 460                 


Ala Leu Phe Glu Glu Leu Asn Asp Ala Glu Gln Leu Glu Leu Asn Gly 
465                 470                 475                 480 


Ser Arg Arg Leu Cys Gln Glu Glu Lys Glu Leu Leu Ala Gln Glu Leu 
                485                 490                 495     


Phe Ile Arg Asp Lys Met Thr Lys Ser Glu Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asp Asn Pro Gln Glu Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn 
        515                 520                 525             


Lys Thr Gly Tyr Ala Leu Phe Gln Ala Tyr Ser Lys Met Ile Glu Met 
    530                 535                 540                 


Ser Gly His Glu Pro Val Asp Phe Lys Lys Pro Val Glu Lys Val Val 
545                 550                 555                 560 


Glu Tyr Ile Lys Ala Val Phe Asp Leu Leu Asn Trp Asn Thr Asp Ile 
                565                 570                 575     


Leu Gly Phe Asn Ser Asn Glu Glu Leu Asp Asn Gln Pro Tyr Tyr Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Arg Leu Ile Gln Lys Met Thr Glu Leu Tyr Gly Phe Glu Lys 
    610                 615                 620                 


Glu Tyr Ala Thr Ile Leu Ala Asn Val Ser Phe Gln Asp Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile His Lys Ile Leu Pro His Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Val Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Glu Ser Ser Leu Thr Arg Glu Glu Ile Ala Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Arg Leu Met Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Val Ile Ile Asp Ile 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Ser Ile Ala Gln Thr 
            740                 745                 750         


Thr Lys Ala His Glu Glu Tyr Lys Thr Leu Leu Gln Thr Glu Phe Gly 
        755                 760                 765             


Leu Thr Asn Val Ser Arg Thr Asp Ile Leu Arg Tyr Lys Leu Tyr Lys 
    770                 775                 780                 


Glu Leu Glu Ser Cys Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Ser Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Ala Arg Ser Val Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Phe Gly Glu Ser Gly Ala Asp Asn Ser Leu Glu His 
    850                 855                 860                 


Tyr Leu Asn Asn Ile Glu Asp Leu Phe Lys Ser Gly Lys Ile Ser Lys 
865                 870                 875                 880 


Thr Lys Tyr Asn Lys Leu Lys Met Ala Glu Gln Asp Ile Pro Asp Gly 
                885                 890                 895     


Phe Ile Glu Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala 
            900                 905                 910         


Leu Ser Met Leu Asn Glu Ile Ser His Arg Val Val Ala Thr Ser Gly 
        915                 920                 925             


Ser Val Thr Asp Lys Leu Arg Glu Asp Trp Gln Leu Ile Asp Val Met 
    930                 935                 940                 


Lys Glu Leu Asn Trp Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr 
945                 950                 955                 960 


Phe Glu Asp Arg Asp Gly Arg Gln Ile Gly Arg Ile Lys Asp Trp Thr 
                965                 970                 975     


Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala 
            980                 985                 990         


Phe Thr Lys Asp Val Phe Ile Gln  Tyr Phe Asn Asn Lys  Asn Ala Ser 
        995                 1000                 1005             


Leu Asp  Pro Asn Ala Asn Glu  His Ala Ile Lys Asn  Lys Tyr Phe 
    1010                 1015                 1020             


Gln Asn  Gly Arg Ala Ile Ala  Pro Met Pro Leu Arg  Glu Phe Arg 
    1025                 1030                 1035             


Ala Glu  Ala Lys Lys His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Ile Thr  Gly Asn Ile Asn Lys  Thr Arg Lys 
    1055                 1060                 1065             


Lys Gly  Gly Val Asn Lys Asn  Met Gln Gln Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Gly Lys Gln  Tyr Leu Thr 
    1085                 1090                 1095             


Lys Glu  Glu Lys Val Asn Ala  Ser Phe Asp Met Arg  Lys Ile Gly 
    1100                 1105                 1110             


Thr Val  Ser Lys Ser Ala Tyr  Arg Asp Ala Leu Leu  Lys Arg Leu 
    1115                 1120                 1125             


Tyr Glu  Asn Asp Asn Asp Pro  Lys Lys Ala Phe Ala  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Asp Lys Gln Pro Ile  Trp Leu Asp Lys Glu  Gln Met Arg 
    1145                 1150                 1155             


Lys Val  Pro Glu Lys Val Lys  Ile Val Thr Leu Glu  Ala Ile Tyr 
    1160                 1165                 1170             


Thr Ile  Arg Lys Glu Ile Ser  Pro Asp Leu Lys Val  Asp Lys Val 
    1175                 1180                 1185             


Ile Asp  Val Gly Val Arg Lys  Ile Leu Ile Asp Arg  Leu Asn Glu 
    1190                 1195                 1200             


Tyr Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Lys Asn 
    1205                 1210                 1215             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1220                 1225                 1230             


Thr Ile  Ser Gly Ile Ser Asn  Ala Gln Ser Leu His  Val Lys Lys 
    1235                 1240                 1245             


Asp Lys  Asp Gly Lys Pro Ile  Leu Asp Glu Asn Gly  Arg Asn Ile 
    1250                 1255                 1260             


Pro Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Val 
    1265                 1270                 1275             


Tyr Tyr  Arg Pro Val Ile Asp  Lys Arg Gly Gln Leu  Val Val Asp 
    1280                 1285                 1290             


Glu Ala  Gly Asn Pro Lys Tyr  Glu Leu Glu Glu Val  Val Val Ser 
    1295                 1300                 1305             


Phe Phe  Glu Ala Val Thr Arg  Ala Asn Leu Gly Leu  Pro Ile Ile 
    1310                 1315                 1320             


Asp Lys  Asp Tyr Lys Thr Thr  Glu Gly Trp Gln Phe  Leu Phe Ser 
    1325                 1330                 1335             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Glu  Lys Thr Gly 
    1340                 1345                 1350             


Phe Asn  Pro Lys Glu Ile Asp  Leu Leu Asp Val Glu  Asn Tyr Gly 
    1355                 1360                 1365             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Phe  Ser Leu Lys 
    1370                 1375                 1380             


Asn Tyr  Val Phe Arg His His  Leu Glu Thr Thr Ile  Lys Asp Thr 
    1385                 1390                 1395             


Ser Ser  Ile Leu Arg Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser 
    1400                 1405                 1410             


Lys Gly  Leu Asp Thr Ile Val  Lys Val Arg Val Asn  His Ile Gly 
    1415                 1420                 1425             


Gln Ile  Val Ser Val Gly Glu  Tyr 
    1430                 1435     


<210>  1248
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1248

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Lys Asp Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Thr 
65                  70                  75                  80  


Leu Thr Glu Val Leu Lys Glu His Lys Leu Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Thr Glu Glu Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Val Glu Glu Gly Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Glu Leu Tyr Asn Asn Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asp Ala Gly Lys Lys Phe Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Val Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Tyr Phe Val Trp Lys Glu Asn 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Lys Glu Lys Gly Lys Thr Glu Gln Gln Glu Arg 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Arg Glu Asn Leu Gln Trp Arg Val Asn Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Val Phe Gln Glu Met Asn Thr Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Tyr Gln Met Glu Met Leu 
                325                 330                 335     


Asp Lys Asn Pro Asn Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Met Leu Trp Glu Lys Gln Ala Val Tyr 
        355                 360                 365             


His Lys Glu Leu Thr Glu Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Asp Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Val Gly Asn Arg Val Ile Ser Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Val Val Gly Lys Lys Arg Lys Arg Arg Lys Leu Lys Glu Asn Tyr Ser 
    450                 455                 460                 


Ala Leu Phe Glu Glu Leu Asn Asp Ala Glu Gln Leu Glu Leu Asn Gly 
465                 470                 475                 480 


Ser Arg Arg Leu Cys Gln Glu Glu Lys Glu Leu Leu Ala Gln Glu Leu 
                485                 490                 495     


Phe Ile Arg Asp Lys Met Thr Lys Ser Glu Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asp Asn Pro Gln Glu Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn 
        515                 520                 525             


Lys Thr Gly Tyr Ala Leu Phe Gln Ala Tyr Ser Lys Met Ile Glu Met 
    530                 535                 540                 


Ser Gly His Glu Pro Val Asp Phe Lys Lys Pro Val Glu Lys Val Val 
545                 550                 555                 560 


Glu Tyr Ile Lys Ala Val Phe Asp Leu Leu Asn Trp Asn Thr Asp Ile 
                565                 570                 575     


Leu Gly Phe Asn Ser Asn Glu Glu Leu Asp Asn Gln Ser Tyr Tyr Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Arg Leu Ile Gln Lys Met Thr Glu Leu Tyr Gly Phe Glu Lys 
    610                 615                 620                 


Glu Tyr Ala Thr Ile Leu Ala Asn Val Ser Phe Gln Asp Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile His Lys Ile Leu Pro His Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Val Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Glu Ser Ser Leu Thr Arg Glu Glu Ile Ala Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Arg Leu Met Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Val Ile Ile Asp Ile 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Ser Ile Ala Gln Thr 
            740                 745                 750         


Thr Lys Ala His Glu Glu Tyr Lys Thr Leu Leu Gln Thr Glu Phe Gly 
        755                 760                 765             


Leu Thr Asn Val Ser Arg Thr Asp Ile Leu Arg Tyr Lys Leu Tyr Lys 
    770                 775                 780                 


Glu Leu Glu Ser Cys Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Ser Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Ala Arg Ser Val Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Phe Gly Glu Ser Gly Ala Asp Asn Ser Leu Glu His 
    850                 855                 860                 


Tyr Leu Asn Asn Ile Glu Asp Leu Phe Lys Ser Gly Lys Ile Ser Lys 
865                 870                 875                 880 


Thr Lys Tyr Asn Lys Leu Lys Met Ala Glu Gln Asp Ile Pro Asp Gly 
                885                 890                 895     


Phe Ile Glu Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala 
            900                 905                 910         


Leu Ser Met Leu Asn Glu Ile Ser His Arg Val Val Ala Thr Ser Gly 
        915                 920                 925             


Ser Val Thr Asp Lys Leu Arg Glu Asp Trp Gln Leu Ile Asp Val Met 
    930                 935                 940                 


Lys Glu Leu Asn Trp Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr 
945                 950                 955                 960 


Phe Glu Asp Arg Asp Gly Arg Gln Ile Gly Arg Ile Lys Asp Trp Thr 
                965                 970                 975     


Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala 
            980                 985                 990         


Phe Thr Lys Asp Val Phe Ile Gln  Tyr Phe Asn Asn Lys  Asn Ala Ser 
        995                 1000                 1005             


Leu Asp  Pro Asn Ala Asn Glu  His Ala Ile Lys Asn  Lys Tyr Phe 
    1010                 1015                 1020             


Gln Asn  Gly Arg Ala Ile Ala  Pro Met Pro Leu Arg  Glu Phe Arg 
    1025                 1030                 1035             


Ala Glu  Ala Lys Lys His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Ile Thr  Gly Asn Ile Asn Lys  Thr Arg Lys 
    1055                 1060                 1065             


Lys Gly  Gly Val Asn Lys Asn  Met Gln Gln Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Gly Lys Gln  Tyr Leu Thr 
    1085                 1090                 1095             


Lys Glu  Glu Lys Val Asn Ala  Ser Phe Asp Met Arg  Lys Ile Gly 
    1100                 1105                 1110             


Thr Val  Ser Lys Leu Ala Tyr  Arg Asp Ala Leu Leu  Lys Arg Leu 
    1115                 1120                 1125             


Tyr Glu  Asn Asp Asn Asp Pro  Lys Lys Ala Phe Ala  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Asp Lys Gln Pro Ile  Trp Leu Asp Lys Glu  Gln Met Arg 
    1145                 1150                 1155             


Lys Val  Pro Glu Lys Val Lys  Ile Val Thr Leu Glu  Ala Ile Tyr 
    1160                 1165                 1170             


Thr Ile  Arg Lys Glu Ile Ser  Pro Asp Leu Lys Val  Asp Lys Val 
    1175                 1180                 1185             


Ile Asp  Val Gly Val Arg Lys  Ile Leu Ile Asp Arg  Leu Asn Glu 
    1190                 1195                 1200             


Tyr Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Glu Asn 
    1205                 1210                 1215             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1220                 1225                 1230             


Thr Ile  Ser Gly Ile Ser Asn  Ala Gln Ser Leu His  Val Lys Lys 
    1235                 1240                 1245             


Asp Lys  Asp Gly Lys Pro Ile  Leu Asp Glu Asn Gly  Arg Asn Ile 
    1250                 1255                 1260             


Pro Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Val 
    1265                 1270                 1275             


Tyr Tyr  Arg Pro Val Ile Asp  Lys Arg Gly Gln Leu  Val Val Asp 
    1280                 1285                 1290             


Glu Ala  Gly Asn Pro Lys Tyr  Glu Leu Glu Glu Val  Val Val Ser 
    1295                 1300                 1305             


Phe Phe  Glu Ala Val Thr Arg  Ala Asn Leu Gly Gln  Pro Ile Ile 
    1310                 1315                 1320             


Asp Lys  Asp Tyr Lys Thr Thr  Glu Gly Trp Gln Phe  Leu Phe Ser 
    1325                 1330                 1335             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Glu  Lys Thr Gly 
    1340                 1345                 1350             


Phe Asn  Pro Lys Glu Ile Asp  Leu Leu Asp Val Glu  Asn Tyr Gly 
    1355                 1360                 1365             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Phe  Ser Leu Lys 
    1370                 1375                 1380             


Asn Tyr  Val Phe Arg His His  Leu Glu Thr Thr Ile  Lys Asp Thr 
    1385                 1390                 1395             


Ser Ser  Ile Leu Arg Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser 
    1400                 1405                 1410             


Lys Gly  Leu Asp Thr Ile Val  Lys Val Arg Val Asn  His Ile Gly 
    1415                 1420                 1425             


Gln Ile  Val Ser Val Gly Glu  Tyr 
    1430                 1435     


<210>  1249
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1249

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Lys Asp Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Thr 
65                  70                  75                  80  


Leu Thr Glu Val Leu Lys Glu His Lys Leu Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Thr Glu Glu Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Val Glu Glu Gly Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Glu Leu Tyr Asn Asn Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asp Ala Gly Lys Lys Phe Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Val Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Tyr Phe Val Trp Lys Glu Thr 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Lys Glu Lys Gly Lys Thr Glu Gln Gln Glu Arg 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Arg Glu Asn Leu Gln Trp Arg Val Asn Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Val Phe Gln Glu Met Asn Thr Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Tyr Gln Met Glu Met Leu 
                325                 330                 335     


Asp Lys Asn Pro Asn Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Met Leu Trp Glu Lys Gln Ala Val Tyr 
        355                 360                 365             


His Lys Glu Leu Thr Glu Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Asp Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Val Gly Asn Arg Val Ile Ser Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Val Val Gly Lys Lys Arg Lys Arg Arg Lys Leu Lys Glu Asn Tyr Ser 
    450                 455                 460                 


Ala Ile Phe Glu Glu Leu Asn Asp Ala Glu Gln Leu Glu Leu Asn Gly 
465                 470                 475                 480 


Ser Arg Arg Leu Cys Gln Glu Glu Lys Glu Leu Leu Ala Gln Glu Leu 
                485                 490                 495     


Phe Val Arg Asp Lys Met Thr Lys Ser Glu Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asp Asn Pro Gln Glu Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn 
        515                 520                 525             


Lys Thr Gly Tyr Ala Leu Phe Gln Ala Tyr Ser Lys Met Ile Glu Met 
    530                 535                 540                 


Ser Gly His Glu Pro Val Asp Phe Lys Lys Pro Val Glu Lys Val Val 
545                 550                 555                 560 


Glu Tyr Ile Lys Ala Val Phe Asp Leu Leu Asn Trp Asn Thr Asp Ile 
                565                 570                 575     


Leu Gly Phe Asn Ser Asn Glu Glu Leu Asp Asn Gln Ser Tyr Tyr Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Arg Leu Ile Gln Lys Met Thr Glu Leu Tyr Gly Phe Glu Lys 
    610                 615                 620                 


Glu Tyr Ala Thr Ile Leu Ala Asn Val Ser Phe Gln Asp Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile His Lys Ile Leu Pro His Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Val Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Glu Ser Ser Leu Thr Arg Glu Glu Ile Ala Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Arg Leu Met Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Val Ile Ile Asp Ile 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Ser Ile Ala Gln Thr 
            740                 745                 750         


Thr Lys Ala Asn Glu Glu Tyr Lys Thr Leu Leu Gln Thr Glu Phe Gly 
        755                 760                 765             


Leu Thr Asn Val Ser Arg Thr Asp Ile Leu Arg Tyr Lys Leu Tyr Lys 
    770                 775                 780                 


Glu Leu Glu Ser Cys Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Ser Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Ala Arg Ser Val Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Phe Gly Glu Ser Gly Ala Asp Asn Ser Leu Glu His 
    850                 855                 860                 


Tyr Leu Asn Asn Ile Glu Asp Leu Phe Lys Ser Gly Lys Ile Ser Lys 
865                 870                 875                 880 


Thr Lys Tyr Asn Lys Leu Lys Met Ala Lys Gln Asp Ile Pro Asp Gly 
                885                 890                 895     


Phe Ile Glu Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala 
            900                 905                 910         


Leu Ser Met Leu Asn Glu Ile Ser His Arg Val Val Ala Thr Ser Gly 
        915                 920                 925             


Ser Val Thr Asp Lys Leu Arg Glu Asp Trp Gln Leu Ile Asp Val Met 
    930                 935                 940                 


Lys Glu Leu Asn Trp Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr 
945                 950                 955                 960 


Phe Glu Asp Arg Asp Gly Arg Gln Ile Gly Arg Ile Lys Asp Trp Thr 
                965                 970                 975     


Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala 
            980                 985                 990         


Phe Thr Lys Asp Val Phe Ile Gln  Tyr Phe Asn Asn Lys  Asn Ala Ser 
        995                 1000                 1005             


Leu Asp  Pro Asn Ala Asn Glu  His Ala Ile Lys Asn  Lys Tyr Phe 
    1010                 1015                 1020             


Gln Asn  Gly Arg Ala Ile Ala  Pro Met Pro Leu Arg  Glu Phe Arg 
    1025                 1030                 1035             


Ala Glu  Ala Lys Lys His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Ile Thr  Gly Asn Ile Asn Lys  Thr Arg Lys 
    1055                 1060                 1065             


Lys Gly  Gly Val Asn Lys Asn  Met Gln Gln Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Gly Lys Gln  Tyr Leu Thr 
    1085                 1090                 1095             


Lys Glu  Glu Arg Val Asn Ala  Ser Phe Asp Met Arg  Lys Ile Glu 
    1100                 1105                 1110             


Thr Val  Ser Lys Ser Ala Tyr  Arg Asp Ala Leu Leu  Lys Arg Leu 
    1115                 1120                 1125             


Tyr Glu  Asn Asp Asn Asp Pro  Lys Lys Ala Phe Ala  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Asp Lys Gln Pro Ile  Trp Leu Asp Lys Glu  Gln Lys Arg 
    1145                 1150                 1155             


Lys Val  Pro Glu Lys Val Lys  Ile Val Thr Leu Glu  Ala Ile Tyr 
    1160                 1165                 1170             


Thr Ile  Arg Lys Glu Ile Ser  Pro Asp Leu Lys Val  Asp Lys Val 
    1175                 1180                 1185             


Ile Asp  Val Gly Val Arg Lys  Ile Leu Ile Asp Arg  Leu Asn Glu 
    1190                 1195                 1200             


Tyr Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Glu Asn 
    1205                 1210                 1215             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1220                 1225                 1230             


Thr Ile  Ser Gly Ile Ser Asn  Ala Gln Ser Leu His  Val Lys Lys 
    1235                 1240                 1245             


Asp Lys  Asp Gly Lys Pro Ile  Leu Asp Glu Asn Gly  Arg Asn Ile 
    1250                 1255                 1260             


Pro Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Val 
    1265                 1270                 1275             


Tyr Tyr  Arg Pro Val Ile Asp  Lys Arg Gly Gln Leu  Val Val Asp 
    1280                 1285                 1290             


Glu Ala  Gly Asn Pro Lys Tyr  Glu Leu Glu Glu Val  Val Val Ser 
    1295                 1300                 1305             


Phe Phe  Glu Ala Val Thr Arg  Ala Asn Leu Gly Gln  Pro Ile Ile 
    1310                 1315                 1320             


Asp Lys  Asp Tyr Lys Thr Thr  Glu Gly Trp Gln Phe  Leu Phe Ser 
    1325                 1330                 1335             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Glu  Lys Thr Gly 
    1340                 1345                 1350             


Phe Asn  Pro Lys Glu Ile Asp  Leu Leu Asp Val Glu  Asn Tyr Gly 
    1355                 1360                 1365             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Phe  Ser Leu Lys 
    1370                 1375                 1380             


Asn Tyr  Val Phe Arg His His  Leu Glu Thr Thr Ile  Lys Asp Thr 
    1385                 1390                 1395             


Ser Ser  Ile Leu Arg Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser 
    1400                 1405                 1410             


Lys Gly  Leu Asp Thr Ile Val  Lys Val Arg Val Asn  His Ile Gly 
    1415                 1420                 1425             


Gln Ile  Val Ser Val Gly Glu  Tyr 
    1430                 1435     


<210>  1250
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1250

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Lys Asp Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Thr 
65                  70                  75                  80  


Leu Thr Glu Val Leu Lys Glu His Lys Leu Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Thr Glu Glu Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Val Glu Glu Gly Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Glu Leu Tyr Asn Asn Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asp Ala Gly Lys Lys Phe Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Val Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Tyr Phe Val Trp Lys Glu Thr 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Lys Glu Lys Gly Lys Thr Glu Gln Gln Glu Lys 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Arg Glu Asn Leu Gln Trp Arg Val Asn Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Val Phe Gln Glu Met Asn Thr Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Tyr Gln Met Glu Met Leu 
                325                 330                 335     


Asp Lys Asn Pro Asn Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Met Leu Trp Glu Lys Gln Ala Val Tyr 
        355                 360                 365             


His Lys Glu Leu Thr Glu Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Asp Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Val Gly Asn Arg Val Ile Ser Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Val Val Gly Lys Lys Arg Lys Arg Arg Lys Leu Lys Glu Asn Tyr Ser 
    450                 455                 460                 


Ala Leu Phe Glu Glu Leu Asn Asp Ala Glu Gln Leu Glu Leu Asn Gly 
465                 470                 475                 480 


Ser Arg Arg Leu Cys Gln Glu Glu Lys Glu Leu Leu Ala Gln Glu Leu 
                485                 490                 495     


Phe Ile Arg Asp Lys Met Thr Lys Ser Glu Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asp Asn Pro Gln Glu Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn 
        515                 520                 525             


Lys Thr Gly Tyr Ala Leu Phe Gln Ala Tyr Ser Lys Met Ile Glu Met 
    530                 535                 540                 


Ser Gly His Glu Pro Val Asp Phe Lys Lys Pro Val Glu Lys Val Val 
545                 550                 555                 560 


Glu Tyr Ile Lys Ala Val Phe Asp Leu Leu Asn Trp Asn Thr Asp Ile 
                565                 570                 575     


Leu Gly Phe Asn Ser Asn Glu Glu Leu Asp Asn Gln Ser Tyr Tyr Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Arg Leu Ile Gln Lys Met Thr Glu Leu Tyr Gly Phe Glu Lys 
    610                 615                 620                 


Glu Tyr Ala Thr Ile Leu Ala Asn Val Ser Phe Gln Asp Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile His Lys Ile Leu Pro His Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Val Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Glu Ser Ser Leu Thr Arg Glu Glu Ile Ala Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Arg Leu Met Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Val Ile Ile Asp Ile 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Ser Ile Ala Gln Thr 
            740                 745                 750         


Thr Lys Ala His Glu Glu Tyr Lys Thr Leu Leu Gln Thr Glu Phe Gly 
        755                 760                 765             


Leu Thr Asn Val Ser Arg Thr Asp Ile Leu Arg Tyr Lys Leu Tyr Lys 
    770                 775                 780                 


Glu Leu Glu Ser Cys Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Ser Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Ala Arg Ser Val Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Phe Gly Glu Ser Gly Ala Asp Asn Ser Leu Glu His 
    850                 855                 860                 


Tyr Leu Asn Asn Ile Glu Asp Leu Phe Lys Ser Gly Lys Ile Ser Lys 
865                 870                 875                 880 


Thr Lys Tyr Asn Lys Leu Lys Met Ala Glu Gln Asp Ile Pro Asp Gly 
                885                 890                 895     


Phe Ile Glu Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala 
            900                 905                 910         


Leu Ser Met Leu Asn Glu Ile Ser His Arg Val Val Ala Thr Ser Gly 
        915                 920                 925             


Ser Val Thr Asp Lys Leu Arg Glu Asp Trp Gln Leu Ile Asp Val Met 
    930                 935                 940                 


Lys Glu Leu Asn Trp Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr 
945                 950                 955                 960 


Phe Glu Asp Arg Asp Gly Arg Gln Ile Gly Arg Ile Lys Asp Trp Thr 
                965                 970                 975     


Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala 
            980                 985                 990         


Phe Thr Lys Asp Val Phe Ile Gln  Tyr Phe Asn Asn Lys  Asn Ala Ser 
        995                 1000                 1005             


Leu Asp  Pro Asn Ala Asn Glu  His Ala Ile Lys Asn  Lys Tyr Phe 
    1010                 1015                 1020             


Gln Asn  Gly Arg Ala Ile Ala  Pro Met Pro Leu Arg  Glu Phe Arg 
    1025                 1030                 1035             


Ala Glu  Ala Lys Lys His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Ile Thr  Gly Asn Ile Asn Lys  Thr Arg Lys 
    1055                 1060                 1065             


Lys Gly  Gly Val Asn Lys Asn  Met Gln Gln Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Gly Lys Gln  Tyr Leu Thr 
    1085                 1090                 1095             


Lys Glu  Glu Lys Val Asn Ala  Ser Phe Asp Met Arg  Lys Ile Gly 
    1100                 1105                 1110             


Thr Val  Ser Lys Leu Ala Tyr  Arg Asp Ala Leu Leu  Lys Arg Leu 
    1115                 1120                 1125             


Tyr Glu  Asn Asp Asn Asp Pro  Lys Lys Ala Phe Ala  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Asp Lys Gln Pro Ile  Trp Leu Asp Lys Glu  Gln Met Arg 
    1145                 1150                 1155             


Lys Val  Pro Glu Lys Val Lys  Ile Val Thr Leu Glu  Ala Ile Tyr 
    1160                 1165                 1170             


Thr Ile  Arg Lys Glu Ile Ser  Pro Asp Leu Lys Val  Asp Lys Val 
    1175                 1180                 1185             


Ile Asp  Val Gly Val Arg Lys  Ile Leu Ile Asp Arg  Leu Asn Glu 
    1190                 1195                 1200             


Tyr Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Glu Asn 
    1205                 1210                 1215             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1220                 1225                 1230             


Thr Ile  Ser Gly Ile Ser Asn  Ala Gln Ser Leu His  Val Lys Lys 
    1235                 1240                 1245             


Asp Lys  Asp Gly Lys Pro Ile  Leu Asp Glu Asn Gly  Arg Asn Ile 
    1250                 1255                 1260             


Pro Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Val 
    1265                 1270                 1275             


Tyr Tyr  Arg Pro Val Ile Asp  Lys Arg Gly Gln Leu  Val Val Asp 
    1280                 1285                 1290             


Glu Ala  Gly Asn Pro Lys Tyr  Glu Leu Glu Glu Val  Val Val Ser 
    1295                 1300                 1305             


Phe Phe  Glu Ala Val Thr Arg  Ala Asn Leu Gly Gln  Pro Ile Ile 
    1310                 1315                 1320             


Asp Lys  Asp Tyr Lys Thr Thr  Glu Gly Trp Gln Phe  Leu Phe Ser 
    1325                 1330                 1335             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Glu  Lys Thr Gly 
    1340                 1345                 1350             


Phe Asn  Pro Lys Glu Ile Asp  Leu Leu Asp Val Glu  Asn Tyr Gly 
    1355                 1360                 1365             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Phe  Ser Leu Lys 
    1370                 1375                 1380             


Asn Tyr  Val Phe Arg His His  Leu Glu Thr Thr Ile  Lys Asp Thr 
    1385                 1390                 1395             


Ser Ser  Ile Leu Arg Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser 
    1400                 1405                 1410             


Lys Gly  Leu Asp Thr Ile Val  Lys Val Arg Val Asn  His Ile Gly 
    1415                 1420                 1425             


Gln Ile  Val Ser Val Gly Glu  Tyr 
    1430                 1435     


<210>  1251
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1251

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Lys Asp Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Thr 
65                  70                  75                  80  


Leu Thr Glu Val Leu Lys Glu His Lys Leu Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Thr Glu Glu Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Val Glu Glu Gly Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Glu Leu Tyr Asn Asn Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asp Ala Gly Lys Lys Phe Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Val Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Tyr Phe Val Trp Lys Glu Asn 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Lys Glu Lys Gly Lys Thr Glu Gln Gln Glu Arg 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Arg Glu Asn Leu Gln Trp Arg Val Asn Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Val Phe Gln Glu Met Asn Thr Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Tyr Gln Met Glu Met Leu 
                325                 330                 335     


Asp Lys Asn Pro Asn Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Met Leu Trp Glu Lys Gln Ala Val Tyr 
        355                 360                 365             


His Lys Glu Leu Thr Glu Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Asp Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Val Gly Asn Arg Val Ile Ser Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Val Val Gly Lys Lys Arg Lys Arg Arg Lys Leu Lys Glu Asn Tyr Ser 
    450                 455                 460                 


Ala Leu Phe Glu Glu Leu Asn Asp Ala Glu Gln Leu Glu Leu Asn Gly 
465                 470                 475                 480 


Ser Arg Arg Leu Cys Gln Glu Glu Lys Glu Leu Leu Ala Gln Glu Leu 
                485                 490                 495     


Phe Ile Arg Asp Lys Met Thr Lys Ser Glu Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asp Asn Pro Gln Glu Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn 
        515                 520                 525             


Lys Thr Gly Tyr Ala Leu Phe Gln Ala Tyr Ser Lys Met Ile Glu Met 
    530                 535                 540                 


Ser Gly His Glu Pro Val Asp Phe Lys Lys Pro Val Glu Lys Val Val 
545                 550                 555                 560 


Glu Tyr Ile Lys Ala Val Phe Asp Leu Leu Asn Trp Asn Thr Asp Ile 
                565                 570                 575     


Leu Gly Phe Asn Ser Asn Glu Glu Leu Asp Asn Gln Pro Tyr Tyr Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Arg Leu Ile Gln Lys Met Thr Glu Leu Tyr Gly Phe Glu Lys 
    610                 615                 620                 


Glu Tyr Ala Thr Ile Leu Ala Asn Val Ser Phe Gln Asp Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile His Lys Ile Leu Pro His Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Val Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Glu Ser Ser Leu Thr Arg Glu Glu Ile Ala Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Arg Leu Met Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Val Ile Ile Asp Ile 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Ser Ile Ala Gln Thr 
            740                 745                 750         


Thr Lys Ala His Glu Glu Tyr Lys Thr Leu Leu Gln Thr Glu Phe Gly 
        755                 760                 765             


Leu Thr Asn Val Ser Arg Thr Asp Ile Leu Arg Tyr Lys Leu Tyr Lys 
    770                 775                 780                 


Glu Leu Glu Ser Cys Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Ser Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Ala Arg Ser Val Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Phe Gly Glu Ser Gly Ala Asp Asn Ser Leu Glu His 
    850                 855                 860                 


Tyr Leu Asn Asn Ile Glu Asp Leu Phe Lys Ser Gly Lys Ile Ser Lys 
865                 870                 875                 880 


Thr Lys Tyr Asn Lys Leu Lys Met Ala Glu Gln Asp Ile Pro Asp Gly 
                885                 890                 895     


Phe Ile Glu Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala 
            900                 905                 910         


Leu Ser Met Leu Asn Glu Ile Ser His Arg Val Val Ala Thr Ser Gly 
        915                 920                 925             


Ser Val Thr Asp Lys Leu Arg Glu Asp Trp Gln Leu Ile Asp Val Met 
    930                 935                 940                 


Lys Glu Leu Asn Trp Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr 
945                 950                 955                 960 


Phe Glu Asp Arg Asp Gly Arg Gln Ile Gly Arg Ile Lys Asp Trp Thr 
                965                 970                 975     


Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala 
            980                 985                 990         


Phe Thr Lys Asp Val Phe Ile Gln  Tyr Phe Asn Asn Lys  Asn Ala Ser 
        995                 1000                 1005             


Leu Asp  Pro Asn Ala Asn Glu  His Ala Ile Lys Asn  Lys Tyr Phe 
    1010                 1015                 1020             


Gln Asn  Gly Arg Ala Ile Ala  Pro Met Pro Leu Arg  Glu Phe Arg 
    1025                 1030                 1035             


Ala Glu  Ala Lys Lys His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Ile Thr  Gly Asn Ile Asn Lys  Thr Arg Lys 
    1055                 1060                 1065             


Lys Gly  Gly Val Asn Lys Asn  Met Gln Gln Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Gly Lys Gln  Tyr Leu Thr 
    1085                 1090                 1095             


Lys Glu  Glu Lys Val Asn Ala  Ser Phe Asp Met Arg  Lys Ile Gly 
    1100                 1105                 1110             


Thr Val  Ser Lys Ser Ala Tyr  Arg Asp Ala Leu Leu  Lys Arg Leu 
    1115                 1120                 1125             


Tyr Glu  Asn Asp Asn Asp Pro  Lys Lys Ala Phe Ala  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Asp Lys Gln Pro Ile  Trp Leu Asp Lys Glu  Gln Met Arg 
    1145                 1150                 1155             


Lys Val  Pro Glu Lys Val Lys  Ile Val Thr Leu Glu  Ala Ile Tyr 
    1160                 1165                 1170             


Thr Ile  Arg Lys Glu Ile Ser  Ser Asp Leu Lys Val  Asp Lys Val 
    1175                 1180                 1185             


Ile Asp  Val Gly Val Arg Lys  Ile Leu Ile Asp Arg  Leu Asn Glu 
    1190                 1195                 1200             


Tyr Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Lys Asn 
    1205                 1210                 1215             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1220                 1225                 1230             


Thr Ile  Ser Gly Ile Ser Asn  Ala Gln Ser Leu His  Val Lys Lys 
    1235                 1240                 1245             


Asp Lys  Asp Gly Lys Pro Ile  Leu Asp Glu Asn Gly  Arg Asn Ile 
    1250                 1255                 1260             


Pro Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Val 
    1265                 1270                 1275             


Tyr Tyr  Arg Pro Val Ile Asp  Lys Arg Gly Gln Leu  Val Val Asp 
    1280                 1285                 1290             


Glu Ala  Gly Asn Pro Lys Tyr  Glu Leu Glu Glu Val  Val Val Ser 
    1295                 1300                 1305             


Phe Phe  Glu Ala Val Thr Arg  Ala Asn Leu Gly Leu  Pro Ile Ile 
    1310                 1315                 1320             


Asp Lys  Asp Tyr Lys Thr Thr  Glu Gly Trp Gln Phe  Leu Phe Ser 
    1325                 1330                 1335             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Glu  Lys Thr Gly 
    1340                 1345                 1350             


Phe Asn  Pro Lys Glu Ile Asp  Leu Leu Asp Val Glu  Asn Tyr Gly 
    1355                 1360                 1365             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Phe  Ser Leu Lys 
    1370                 1375                 1380             


Asn Tyr  Val Phe Arg His His  Leu Glu Thr Thr Ile  Lys Asp Thr 
    1385                 1390                 1395             


Ser Ser  Ile Leu Arg Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser 
    1400                 1405                 1410             


Lys Gly  Leu Asp Thr Ile Val  Lys Val Arg Val Asn  His Ile Gly 
    1415                 1420                 1425             


Gln Ile  Val Ser Val Gly Glu  Tyr 
    1430                 1435     


<210>  1252
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1252

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Lys Asp Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Thr 
65                  70                  75                  80  


Leu Thr Glu Val Leu Lys Glu His Lys Leu Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Thr Glu Glu Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Val Glu Glu Gly Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Glu Leu Tyr Asn Asn Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asp Ala Gly Lys Lys Phe Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Val Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Tyr Phe Val Trp Lys Glu Asn 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Lys Glu Lys Gly Lys Thr Glu Gln Gln Glu Arg 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Arg Glu Asn Leu Gln Trp Arg Val Asn Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Val Phe Gln Glu Met Asn Thr Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Cys Gln Met Glu Met Leu 
                325                 330                 335     


Asp Lys Asn Pro Asn Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Met Leu Trp Glu Lys Gln Ala Val Tyr 
        355                 360                 365             


His Lys Glu Leu Thr Glu Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Asp Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Val Gly Asn Arg Val Ile Ser Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Val Val Gly Lys Lys Arg Lys Arg Arg Lys Leu Lys Glu Asn Tyr Ser 
    450                 455                 460                 


Ala Leu Phe Glu Glu Leu Asn Asp Ala Glu Gln Leu Glu Leu Asn Gly 
465                 470                 475                 480 


Ser Arg Arg Leu Cys Gln Glu Glu Lys Glu Leu Leu Ala Gln Glu Leu 
                485                 490                 495     


Phe Ile Arg Asp Lys Met Thr Lys Ser Glu Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asp Asn Pro Gln Glu Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn 
        515                 520                 525             


Lys Thr Gly Tyr Ala Leu Phe Gln Ala Tyr Ser Lys Met Ile Glu Met 
    530                 535                 540                 


Ser Gly His Glu Pro Val Asp Phe Lys Lys Pro Val Glu Lys Val Val 
545                 550                 555                 560 


Glu Tyr Ile Lys Ala Val Phe Asp Leu Leu Asn Trp Asn Thr Asp Ile 
                565                 570                 575     


Leu Gly Phe Asn Ser Asn Glu Glu Leu Asp Asn Gln Pro Tyr Tyr Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Arg Leu Ile Gln Lys Met Thr Glu Leu Tyr Gly Phe Glu Lys 
    610                 615                 620                 


Glu Tyr Ala Thr Ile Leu Ala Asn Val Ser Phe Gln Asp Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile His Lys Ile Leu Pro His Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Val Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Glu Ser Ser Leu Thr Arg Glu Glu Ile Ala Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Arg Leu Met Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Val Ile Ile Asp Ile 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Ser Ile Ala Gln Thr 
            740                 745                 750         


Thr Lys Ala His Glu Glu Tyr Lys Thr Leu Leu Gln Thr Glu Phe Gly 
        755                 760                 765             


Leu Thr Asn Val Ser Arg Thr Asp Ile Leu Arg Tyr Lys Leu Tyr Lys 
    770                 775                 780                 


Glu Leu Glu Ser Cys Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Ser Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Ala Arg Ser Val Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Phe Gly Glu Ser Gly Ala Asp Asn Ser Leu Glu His 
    850                 855                 860                 


Tyr Leu Asn Asn Ile Glu Asp Leu Phe Lys Ser Gly Lys Ile Ser Lys 
865                 870                 875                 880 


Thr Lys Tyr Asn Lys Leu Lys Met Ala Glu Gln Asp Ile Pro Asp Gly 
                885                 890                 895     


Phe Ile Glu Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala 
            900                 905                 910         


Leu Ser Met Leu Asn Glu Ile Ser His Arg Val Val Ala Thr Ser Gly 
        915                 920                 925             


Ser Val Thr Asp Lys Leu Arg Glu Asp Trp Gln Leu Ile Asp Val Met 
    930                 935                 940                 


Lys Glu Leu Asn Trp Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr 
945                 950                 955                 960 


Phe Glu Asp Arg Asp Gly Arg Gln Ile Gly Arg Ile Lys Asp Trp Thr 
                965                 970                 975     


Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala 
            980                 985                 990         


Phe Thr Lys Asp Val Phe Ile Gln  Tyr Phe Asn Asn Lys  Asn Ala Ser 
        995                 1000                 1005             


Leu Asp  Pro Asn Ala Asn Glu  His Ala Ile Lys Asn  Lys Tyr Phe 
    1010                 1015                 1020             


Gln Asn  Gly Arg Ala Ile Ala  Pro Met Pro Leu Arg  Glu Phe Arg 
    1025                 1030                 1035             


Ala Glu  Ala Lys Lys His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Ile Thr  Gly Asn Ile Asn Lys  Thr Arg Lys 
    1055                 1060                 1065             


Lys Gly  Gly Val Asn Lys Asn  Met Gln Gln Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Gly Lys Gln  Tyr Leu Thr 
    1085                 1090                 1095             


Lys Glu  Glu Lys Val Asn Ala  Ser Phe Asp Met Arg  Lys Ile Gly 
    1100                 1105                 1110             


Thr Val  Ser Lys Ser Ala Tyr  Arg Asp Ala Leu Leu  Lys Arg Leu 
    1115                 1120                 1125             


Tyr Glu  Asn Asp Asn Asp Pro  Lys Lys Ala Phe Ala  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Asp Lys Gln Pro Ile  Trp Leu Asp Lys Glu  Gln Met Arg 
    1145                 1150                 1155             


Lys Val  Pro Glu Lys Val Lys  Ile Val Thr Leu Glu  Ala Ile Tyr 
    1160                 1165                 1170             


Thr Ile  Arg Lys Glu Ile Ser  Pro Asp Leu Lys Val  Asp Lys Val 
    1175                 1180                 1185             


Ile Asp  Val Gly Val Arg Lys  Ile Leu Ile Asp Arg  Leu Asn Glu 
    1190                 1195                 1200             


Tyr Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Lys Asn 
    1205                 1210                 1215             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1220                 1225                 1230             


Thr Ile  Ser Gly Ile Ser Asn  Ala Gln Ser Leu His  Val Lys Lys 
    1235                 1240                 1245             


Asp Lys  Asp Gly Lys Pro Ile  Leu Asp Glu Asn Gly  Arg Asn Ile 
    1250                 1255                 1260             


Pro Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Val 
    1265                 1270                 1275             


Tyr Tyr  Arg Pro Val Ile Asp  Lys Arg Gly Gln Leu  Val Val Asp 
    1280                 1285                 1290             


Glu Ala  Gly Asn Pro Lys Tyr  Glu Leu Glu Glu Val  Val Val Ser 
    1295                 1300                 1305             


Phe Phe  Glu Ala Val Thr Arg  Ala Asn Leu Gly Leu  Pro Ile Ile 
    1310                 1315                 1320             


Asp Lys  Asp Tyr Lys Thr Thr  Glu Gly Trp Gln Phe  Leu Phe Ser 
    1325                 1330                 1335             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Glu  Lys Thr Gly 
    1340                 1345                 1350             


Phe Asn  Pro Lys Glu Ile Asp  Leu Leu Asp Val Glu  Asn Tyr Gly 
    1355                 1360                 1365             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Phe  Ser Leu Lys 
    1370                 1375                 1380             


Asn Tyr  Val Phe Arg His His  Leu Glu Thr Thr Ile  Lys Asp Thr 
    1385                 1390                 1395             


Ser Ser  Ile Leu Arg Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser 
    1400                 1405                 1410             


Lys Gly  Leu Asp Thr Ile Val  Lys Val Arg Val Asn  His Ile Gly 
    1415                 1420                 1425             


Gln Ile  Val Ser Val Gly Glu  Tyr 
    1430                 1435     


<210>  1253
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1253

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Ala Glu Asn Lys Asp Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Thr 
65                  70                  75                  80  


Leu Thr Glu Val Leu Lys Glu His Lys Leu Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Val Thr Glu Glu Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Gly Val Glu Glu Gly Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Glu Leu Tyr Asn Asn Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asp Ala Gly Lys Lys Phe Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Asp Val Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Tyr Phe Val Trp Lys Glu Thr 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Lys Glu Lys Gly Lys Thr Glu Gln Gln Glu Arg 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Arg Glu Asn Leu Gln Trp Arg Val Asn Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Val Phe Gln Glu Met Asn Thr Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Tyr Gln Met Glu Met Leu 
                325                 330                 335     


Asp Lys Asn Pro Asn Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Met Leu Trp Glu Lys Gln Ala Val Tyr 
        355                 360                 365             


His Lys Glu Leu Thr Glu Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Asp Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Val Gly Asn Arg Val Ile Ser Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Val Val Gly Lys Lys Arg Lys Arg Arg Lys Leu Lys Glu Asn Tyr Ser 
    450                 455                 460                 


Ala Ile Phe Glu Glu Leu Asn Asp Ala Glu Gln Leu Glu Leu Asn Gly 
465                 470                 475                 480 


Ser Arg Arg Leu Cys Gln Glu Glu Lys Glu Leu Leu Ala Gln Glu Leu 
                485                 490                 495     


Phe Val Arg Asp Lys Met Thr Lys Ser Glu Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asp Asn Pro Gln Glu Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn 
        515                 520                 525             


Lys Thr Gly Tyr Ala Leu Phe Gln Ala Tyr Ser Lys Met Ile Glu Met 
    530                 535                 540                 


Ser Gly His Glu Pro Val Asp Phe Lys Lys Pro Val Glu Lys Val Val 
545                 550                 555                 560 


Glu Tyr Ile Lys Ala Val Phe Asp Leu Leu Asn Trp Asn Thr Asp Ile 
                565                 570                 575     


Leu Gly Phe Asn Ser Asn Glu Glu Leu Asp Asn Gln Ser Tyr Tyr Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Gly Arg Leu Ile Gln Lys Met Thr Glu Leu Tyr Gly Phe Glu Lys 
    610                 615                 620                 


Glu Tyr Ala Thr Ile Leu Ala Asn Val Ser Phe Gln Asp Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile His Lys Ile Leu Pro His Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Val Tyr Ala Gly Tyr Arg His Ser 
            660                 665                 670         


Glu Ser Ser Leu Thr Arg Glu Glu Ile Ala Asn Lys Val Leu Lys Asp 
        675                 680                 685             


Arg Leu Met Leu Leu Pro Lys Asn Ser Leu His Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Val Ile Ile Asp Ile 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Ser Ile Ala Gln Thr 
            740                 745                 750         


Thr Lys Ala Asn Glu Glu Tyr Lys Thr Leu Leu Gln Thr Glu Phe Gly 
        755                 760                 765             


Leu Thr Asn Val Ser Arg Thr Asp Ile Leu Arg Tyr Lys Leu Tyr Lys 
    770                 775                 780                 


Glu Leu Glu Ser Cys Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Ser Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Ala Arg Ser Val Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Phe Gly Glu Ser Gly Ala Asp Asn Ser Leu Glu His 
    850                 855                 860                 


Tyr Leu Asn Asn Ile Glu Asp Leu Phe Lys Ser Gly Lys Ile Ser Lys 
865                 870                 875                 880 


Thr Lys Tyr Asn Lys Leu Lys Met Ala Glu Gln Asp Ile Pro Asp Gly 
                885                 890                 895     


Phe Ile Glu Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala 
            900                 905                 910         


Leu Ser Met Leu Asn Glu Ile Ser His Arg Val Val Ala Thr Ser Gly 
        915                 920                 925             


Ser Val Thr Asp Lys Leu Arg Glu Asp Trp Gln Leu Ile Asp Val Met 
    930                 935                 940                 


Lys Glu Leu Asn Trp Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr 
945                 950                 955                 960 


Phe Glu Asp Arg Asp Gly Arg Gln Ile Gly Arg Ile Lys Asp Trp Thr 
                965                 970                 975     


Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala 
            980                 985                 990         


Phe Thr Lys Asp Val Phe Ile Gln  Tyr Phe Asn Asn Lys  Asn Ala Ser 
        995                 1000                 1005             


Leu Asp  Pro Asn Ala Asn Glu  His Ala Ile Lys Asn  Lys Tyr Phe 
    1010                 1015                 1020             


Gln Asn  Gly Arg Ala Ile Ala  Pro Met Pro Leu Arg  Glu Phe Arg 
    1025                 1030                 1035             


Ala Glu  Ala Lys Lys His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Ile Thr  Gly Asn Ile Asn Lys  Thr Arg Lys 
    1055                 1060                 1065             


Lys Gly  Gly Val Asn Lys Asn  Met Gln Gln Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Gly Lys Gln  Tyr Leu Thr 
    1085                 1090                 1095             


Lys Glu  Glu Lys Val Asn Ala  Ser Phe Asp Met Arg  Lys Ile Glu 
    1100                 1105                 1110             


Thr Val  Ser Lys Ser Ala Tyr  Arg Asp Ala Leu Leu  Lys Arg Leu 
    1115                 1120                 1125             


Tyr Glu  Asn Asp Asn Asp Pro  Lys Lys Ala Phe Ala  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Asp Lys Gln Pro Ile  Trp Leu Asp Lys Glu  Gln Met Arg 
    1145                 1150                 1155             


Lys Val  Pro Glu Lys Val Lys  Ile Val Thr Leu Glu  Ala Ile Tyr 
    1160                 1165                 1170             


Thr Ile  Arg Lys Glu Ile Ser  Pro Asp Leu Lys Val  Asp Lys Val 
    1175                 1180                 1185             


Ile Asp  Val Gly Val Arg Lys  Ile Leu Ile Asp Arg  Leu Asn Glu 
    1190                 1195                 1200             


Tyr Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Lys Asn 
    1205                 1210                 1215             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1220                 1225                 1230             


Thr Ile  Ser Gly Ile Ser Asn  Ala Gln Ser Leu His  Val Lys Lys 
    1235                 1240                 1245             


Asp Lys  Asp Gly Lys Pro Ile  Leu Asp Glu Asn Gly  Arg Asn Ile 
    1250                 1255                 1260             


Pro Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Val 
    1265                 1270                 1275             


Tyr Tyr  Arg Pro Val Ile Asp  Lys Arg Gly Gln Leu  Val Val Asp 
    1280                 1285                 1290             


Glu Ala  Gly Asn Pro Lys Tyr  Glu Leu Glu Glu Val  Val Val Ser 
    1295                 1300                 1305             


Phe Phe  Glu Ala Val Thr Arg  Ala Asn Leu Gly Leu  Pro Ile Ile 
    1310                 1315                 1320             


Asp Lys  Asp Tyr Lys Thr Thr  Glu Gly Trp Gln Phe  Leu Phe Ser 
    1325                 1330                 1335             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Glu  Lys Ile Gly 
    1340                 1345                 1350             


Phe Asn  Pro Lys Glu Ile Asp  Leu Leu Asp Val Glu  Asn Tyr Gly 
    1355                 1360                 1365             


Leu Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Phe  Ser Leu Lys 
    1370                 1375                 1380             


Asn Tyr  Val Phe Arg His His  Leu Glu Thr Thr Ile  Lys Asp Thr 
    1385                 1390                 1395             


Ser Ser  Ile Leu Arg Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser 
    1400                 1405                 1410             


Lys Gly  Leu Asp Thr Ile Val  Lys Val Arg Val Asn  His Ile Gly 
    1415                 1420                 1425             


Gln Ile  Val Ser Val Gly Glu  Tyr 
    1430                 1435     


<210>  1254
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1254

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asp Glu Ala Glu Asn Lys Asp Glu Arg Ser Ser Ile Val 
            20                  25                  30          


Lys Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Leu Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Gly Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Glu Thr 
65                  70                  75                  80  


Leu Met Glu Val Leu Lys Glu His Lys Leu Ile Thr Glu Asp Thr Ile 
                85                  90                  95      


Leu Ser Glu Asn Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg 
            100                 105                 110         


Ala Lys Ala Ala Thr Glu Lys Ile Ser Leu Glu Glu Phe Ala Arg Val 
        115                 120                 125             


Leu Leu Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Cys Arg Lys Ala 
    130                 135                 140                 


Lys Gly Ala Glu Glu Gly Thr Leu Ile Asp Gly Met Asp Ile Ala Arg 
145                 150                 155                 160 


Glu Leu Tyr Asn Asn Asn Leu Thr Pro Gly Glu Leu Cys Leu Gln Leu 
                165                 170                 175     


Leu Asp Ala Gly Lys Lys Tyr Leu Pro Asp Phe Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Asn Glu Leu Asp Arg Ile Trp Glu Lys Gln Lys Glu Tyr Tyr Pro 
        195                 200                 205             


Glu Ile Leu Thr Glu Val Leu Lys Glu Glu Leu Arg Gly Lys Lys Arg 
    210                 215                 220                 


Asp Ala Val Trp Ala Ile Cys Ala Lys Ser Phe Val Trp Glu Glu Tyr 
225                 230                 235                 240 


Tyr Thr Glu Trp Asn Lys Glu Lys Gly Lys Thr Glu Gln Gln Glu Arg 
                245                 250                 255     


Glu His Lys Leu Glu Gly Ile Tyr Ser Lys Arg Lys Arg Asp Glu Ala 
            260                 265                 270         


Lys Arg Glu Asn Leu Gln Trp Arg Val Asn Gly Leu Lys Glu Lys Leu 
        275                 280                 285             


Ser Leu Glu Gln Leu Val Ile Val Leu Gln Glu Met Asn Ile Gln Ile 
    290                 295                 300                 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
305                 310                 315                 320 


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Tyr Gln Met Glu Met Leu 
                325                 330                 335     


Asp Lys Asn Pro Asn Ala Ser Leu Arg Asn Met Val Phe Tyr Arg Gln 
            340                 345                 350         


Asp Tyr Leu Asp Glu Phe Asn Thr Leu Trp Glu Lys Gln Ala Glu Tyr 
        355                 360                 365             


His Lys Glu Leu Thr Gly Glu Leu Lys Lys Glu Ile Arg Asp Ile Val 
    370                 375                 380                 


Ile Phe Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Val Gly Phe 
385                 390                 395                 400 


Cys Glu Phe Glu Ser Arg Gln Ile Glu Val Glu Ile Asp Gly Lys Lys 
                405                 410                 415     


Lys Ile Lys Thr Val Gly Asn Arg Val Ile Pro Arg Ser Ser Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Lys Ile Trp Gln Ile Leu Asn Asn Ile Glu Val Thr 
        435                 440                 445             


Val Val Gly Lys Lys Gly Lys Arg Arg Lys Gln Lys Asp Asn Ser Pro 
    450                 455                 460                 


Thr Leu Phe Asp Gly Leu Asp Asp Thr Glu Gln Leu Glu Leu Asn Gly 
465                 470                 475                 480 


Ser Arg Tyr Leu Tyr Gln Glu Glu Lys Glu Leu Leu Ala Gln Glu Leu 
                485                 490                 495     


Phe Val Arg Asp Lys Met Thr Lys Ser Asp Val Leu Lys Leu Leu Phe 
            500                 505                 510         


Asp Asn Pro Gln Glu Leu Asp Leu Asn Phe Lys Thr Ile Asp Gly Asn 
        515                 520                 525             


Lys Thr Gly Tyr Thr Leu Phe Gln Ala Tyr Ser Lys Met Ile Glu Met 
    530                 535                 540                 


Ser Gly His Glu Pro Val Asp Phe Lys Lys Thr Val Glu Lys Val Val 
545                 550                 555                 560 


Glu His Val Lys Ala Val Phe Asp Leu Leu Asn Trp Asn Thr Asp Ile 
                565                 570                 575     


Leu Gly Phe Asn Ser Asn Glu Glu Leu Asp Asn Gln Ser Tyr Tyr Lys 
            580                 585                 590         


Leu Trp His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly 
        595                 600                 605             


Asn Ala Arg Leu Val Gln Lys Ile Thr Glu Leu Tyr Gly Phe Glu Lys 
    610                 615                 620                 


Glu Tyr Ala Ile Ile Leu Ala Asn Val Thr Phe Gln Asp Asp Tyr Gly 
625                 630                 635                 640 


Ser Leu Ser Ala Lys Ala Ile His Lys Ile Leu Pro His Leu Lys Glu 
                645                 650                 655     


Gly Asn Arg Tyr Asp Val Ala Cys Ala Tyr Ala Gly Tyr Lys His Ser 
            660                 665                 670         


Glu Ser Ser Leu Thr Lys Glu Glu Ile Ala Asn Lys Ala Leu Lys Asp 
        675                 680                 685             


Arg Leu Thr Leu Leu Pro Lys Asn Asn Leu Arg Asn Pro Val Val Glu 
    690                 695                 700                 


Lys Ile Leu Asn Gln Met Val Asn Val Ile Asn Ala Ile Ile Asp Thr 
705                 710                 715                 720 


Tyr Gly Lys Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys 
                725                 730                 735     


Lys Asn Ala Lys Glu Arg Glu Glu Leu Thr Lys Phe Ile Ala Glu Thr 
            740                 745                 750         


Thr Arg Lys His Glu Glu Phe Lys Ile Leu Leu Gln Ser Glu Phe Gly 
        755                 760                 765             


Leu Thr Asn Val Ser Arg Thr Asp Ile Leu Arg Tyr Lys Leu Tyr Lys 
    770                 775                 780                 


Glu Leu Glu Ser Cys Gly Tyr Lys Thr Leu Tyr Ser Asn Thr Tyr Ile 
785                 790                 795                 800 


Pro Arg Glu Lys Leu Phe Ser Lys Glu Phe Asp Ile Glu His Ile Ile 
                805                 810                 815     


Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
            820                 825                 830         


Ala Arg Ser Ile Asn Leu Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe 
        835                 840                 845             


Val Lys Glu Lys Phe Gly Glu Ser Gly Ala Asp Asn Ser Leu Asp His 
    850                 855                 860                 


Tyr Leu Asn Asn Ile Glu Asp Leu Phe Lys Ser Gly Lys Ile Ser Lys 
865                 870                 875                 880 


Thr Lys Tyr Asn Lys Leu Lys Met Ala Glu Gln Asp Ile Pro Asp Gly 
                885                 890                 895     


Phe Ile Asn Arg Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala 
            900                 905                 910         


Leu Ser Met Leu Asn Glu Ile Ser Arg Arg Val Val Ala Thr Ser Gly 
        915                 920                 925             


Thr Ile Thr Asp Lys Leu Arg Gln Asp Trp Gln Leu Val Asp Val Met 
    930                 935                 940                 


Lys Glu Leu Asn Trp Glu Lys Tyr Lys Ala Leu Gly Leu Val Glu Tyr 
945                 950                 955                 960 


Phe Glu Asp Lys Asp Gly Arg Gln Ile Gly Arg Ile Lys Asp Trp Thr 
                965                 970                 975     


Lys Arg Asn Asp His Arg His His Ala Met Asp Ala Leu Thr Val Ala 
            980                 985                 990         


Phe Thr Lys Asp Ile Phe Ile Gln  Tyr Phe Asn Asn Lys  Asn Ala Ser 
        995                 1000                 1005             


Leu Asn  Ser Asn Thr Asn Glu  Tyr Ala Ile Lys Asn  Lys Tyr Phe 
    1010                 1015                 1020             


Gln Asn  Gly Arg Ala Ile Ala  Pro Ile Pro Leu Arg  Glu Phe Arg 
    1025                 1030                 1035             


Val Glu  Ala Lys Lys His Leu  Glu Asn Thr Leu Ile  Ser Ile Lys 
    1040                 1045                 1050             


Ala Lys  Asn Lys Val Val Thr  Gly Asn Ile Asn Lys  Thr Lys Lys 
    1055                 1060                 1065             


Arg Gly  Gly Val Asn Lys Tyr  Leu Gln Gln Thr Pro  Arg Gly Gln 
    1070                 1075                 1080             


Leu His  Leu Glu Thr Ile Tyr  Gly Ser Gly Lys Gln  Tyr Leu Thr 
    1085                 1090                 1095             


Lys Glu  Glu Lys Val Asn Ala  Ser Phe Asp Met Lys  Lys Ile Ala 
    1100                 1105                 1110             


Thr Val  Ser Lys Ser Ala Tyr  Arg Asp Val Leu Leu  Lys Arg Leu 
    1115                 1120                 1125             


His Glu  Asn Asp Asn Asp Pro  Lys Lys Ala Phe Thr  Gly Lys Asn 
    1130                 1135                 1140             


Ser Leu  Asp Lys Gln Pro Ile  Trp Leu Asp Lys Glu  Gln Thr Arg 
    1145                 1150                 1155             


Lys Val  Pro Glu Lys Val Lys  Thr Val Thr Leu Glu  Thr Ile Tyr 
    1160                 1165                 1170             


Thr Val  Arg Lys Glu Ile Ser  Pro Asp Leu Lys Val  Asp Lys Val 
    1175                 1180                 1185             


Ile Asp  Ala Gly Val Arg Lys  Ile Leu Thr Gly Arg  Leu Asn Glu 
    1190                 1195                 1200             


Tyr Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asn Leu  Asp Glu Asn 
    1205                 1210                 1215             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ser Ile  Lys Arg Val 
    1220                 1225                 1230             


Thr Ile  Ser Gly Ile Ser Asn  Ala Gln Ser Leu His  Val Lys Lys 
    1235                 1240                 1245             


Asp Lys  Asp Gly Lys Pro Ile  Leu Asp Glu Asp Ser  Arg Asn Ile 
    1250                 1255                 1260             


Pro Val  Asp Phe Val Asn Thr  Gly Asn Asn His His  Val Ala Val 
    1265                 1270                 1275             


Tyr Arg  Arg Pro Val Ile Asp  Lys Arg Gly Gln Leu  Val Ile Asp 
    1280                 1285                 1290             


Glu Ala  Gly Asn Pro Lys Tyr  Glu Leu Glu Glu Val  Val Val Ser 
    1295                 1300                 1305             


Phe Phe  Glu Ala Val Thr Arg  Ala Asn Leu Gly Gln  Pro Ile Ile 
    1310                 1315                 1320             


Asp Lys  Asp Tyr Lys Thr Ser  Glu Gly Trp Gln Phe  Leu Phe Ser 
    1325                 1330                 1335             


Met Lys  Gln Asn Glu Tyr Phe  Val Phe Pro Asn Glu  Lys Thr Gly 
    1340                 1345                 1350             


Phe Asn  Pro Lys Glu Ile Asp  Leu Leu Asp Ala Glu  Asn Tyr Gly 
    1355                 1360                 1365             


Val Ile  Ser Pro Asn Leu Phe  Arg Val Gln Lys Phe  Ala His Lys 
    1370                 1375                 1380             


Asn Tyr  Val Phe Arg His His  Leu Glu Thr Thr Ile  Lys Asp Thr 
    1385                 1390                 1395             


Ser Ser  Ile Leu Lys Gly Ile  Thr Trp Ile Asp Phe  Arg Ser Ser 
    1400                 1405                 1410             


Lys Gly  Leu Asp Ala Ile Val  Lys Val Arg Val Asn  His Ile Gly 
    1415                 1420                 1425             


Gln Ile  Val Ser Val Gly Glu  Tyr 
    1430                 1435     


<210>  1255
<211>  1436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1255

Met Lys Tyr Ile Lys Tyr Ile Leu Gly Leu Asp Ile Gly Ser Asn Ser 
1               5                   10                  15      


Ile Gly Trp Ala Leu Ile Gln Leu Asp Leu Leu Gln Lys Thr Lys Arg 
            20                  25                  30          


Ile Ile Ala Ala Gly Ser Arg Ile Ile Pro Met Asp Gln Lys Val Leu 
        35                  40                  45              


Ser Asp Phe Gly Lys Gly Ile Thr Ile Ser Pro Thr Ala Gln Arg Thr 
    50                  55                  60                  


Ser Tyr Arg Asn Ala Arg His Cys Arg Glu Arg His Leu Leu Arg Arg 
65                  70                  75                  80  


Glu Arg Leu His Arg Val Leu His Ile Leu Gly Phe Leu Pro Thr His 
                85                  90                  95      


Tyr Asp Lys Gln Ile Asp Phe Ser Lys Arg Tyr Gly Lys Phe Val Asp 
            100                 105                 110         


Asn Thr Glu Pro Lys Ile Ala Tyr Asn Glu Gly Lys Phe Leu Phe Met 
        115                 120                 125             


Asp Ser Phe Gln Glu Met Leu Ser Asp Phe Lys Ser His Gln Pro Asp 
    130                 135                 140                 


Leu Phe Tyr Thr Lys Lys Asn Gly Glu Glu Ser Lys Ile Pro Tyr Asp 
145                 150                 155                 160 


Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Lys Ile Ser 
                165                 170                 175     


Lys Glu Glu Leu Ala Trp Ile Ile Leu Asn Phe Asn Gln Lys Arg Gly 
            180                 185                 190         


Tyr Tyr Gln Leu Arg Glu Glu Glu Gly Asn Lys Glu Asn Asp Phe Glu 
        195                 200                 205             


Ile Ile Ser Ser Lys Ile Ile Ser Val Ile Lys Lys Asp Lys Asp Lys 
    210                 215                 220                 


Lys Tyr Asn Lys Tyr Trp Tyr Asp Ile Ile Leu Glu Asn Gly Leu Val 
225                 230                 235                 240 


Tyr Gln Ala Ala Phe Tyr Asn Asp Phe Val Tyr Asn Trp Ile Asn Gln 
                245                 250                 255     


Asp Arg Glu Phe Ile Ile Glu Arg Thr Ser Leu Lys Asn Gly Ala Glu 
            260                 265                 270         


Lys Val Lys Leu Ser Tyr Leu Pro Thr Phe Asp Glu Ile Asp Lys Met 
        275                 280                 285             


Asp Lys Lys Glu Gln Asp Lys Tyr Tyr Lys Lys Ile Lys Ile Lys Thr 
    290                 295                 300                 


Glu Asn Ser Ile Gly Asn Lys Thr Val Gly Cys Tyr Ile Tyr Asp His 
305                 310                 315                 320 


Ile Leu Gln Asn Pro Asn Glu Lys Ile Lys Gly Lys Leu Val Arg Thr 
                325                 330                 335     


Ile Glu Arg Lys Tyr Tyr Lys Glu Glu Leu Lys Ala Ile Leu Glu Lys 
            340                 345                 350         


Gln Ile Ala Leu Gln Pro Glu Leu Phe Thr Asp Gln Leu Leu Ala Asp 
        355                 360                 365             


Cys Ile Arg Glu Leu Tyr Ser Lys Asn Glu Ala Gln Gln Arg Asn Leu 
    370                 375                 380                 


Ala Ala Arg Asp Phe Val His Leu Leu Val Glu Asp Ile Ile Phe Tyr 
385                 390                 395                 400 


Gln Arg Pro Leu Arg Arg Gln Lys Ser Thr Leu Ala Asn Cys Ser Leu 
                405                 410                 415     


Glu Ser Arg Ser Tyr Ile Asp Lys Asp Ser His Thr Arg Lys Glu Ala 
            420                 425                 430         


Pro Leu Lys Val Cys Pro Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg 
        435                 440                 445             


Val Leu Gln Trp Leu Gln Asn Leu Lys Ile Tyr Glu Ile Asp Ser Asp 
    450                 455                 460                 


Gln Glu Val Thr His Gln Phe Ile Lys Thr Leu Glu Asp Lys Gln Gln 
465                 470                 475                 480 


Leu Phe Glu Phe Leu Met Ala Gln Lys Glu Ile Asp Cys Glu Glu Leu 
                485                 490                 495     


Leu Lys Tyr Phe Leu Asn Gln Thr Tyr Pro Asn Ala Lys Glu Lys Ala 
            500                 505                 510         


Leu Lys Ser Glu Leu Lys Lys Trp Lys Asp Thr Tyr Arg Trp Asn Tyr 
        515                 520                 525             


Val Tyr Asp Ile Gly Glu Lys Ser Ser Lys Lys Tyr Pro Met Asn Glu 
    530                 535                 540                 


Thr Arg Tyr Glu Leu Lys Arg His Leu Glu Lys Val Ala Asn Leu Pro 
545                 550                 555                 560 


Asp Asp Phe Leu Ser Ser Glu Val Glu Tyr Leu Leu Trp His Leu Ile 
                565                 570                 575     


Tyr Ser Val Lys Asp Lys Val Ala Tyr Glu Lys Gly Leu Lys Lys Phe 
            580                 585                 590         


Ala Gln Lys His His Leu Asp Lys Asp Ser Phe Val Glu Ser Phe Lys 
        595                 600                 605             


Lys Phe Asn Pro Tyr Pro Ser Glu Tyr Gly Ser Phe Ser Glu Lys Ala 
    610                 615                 620                 


Ile Arg Lys Leu Leu Pro Leu Met Arg Phe Gly Ala His Trp Asp Phe 
625                 630                 635                 640 


Asn His Ile Asp Lys Asn Thr Gln Lys Arg Ile Glu Asp Leu Ile Thr 
                645                 650                 655     


Gly Val Glu Asn Glu Glu Ile Arg Thr Ile Leu Arg Glu Lys Ala Glu 
            660                 665                 670         


Lys Tyr Gln Leu Glu Lys Glu Thr Asp Phe Gln Asp Leu Pro Leu Trp 
        675                 680                 685             


Leu Ala Gln Tyr Ile Val Tyr Asn Arg His Ala Glu Ala Ser Ser Leu 
    690                 695                 700                 


Glu Lys Trp Thr Ser Val Asn Asp Leu Glu Thr Tyr Leu Asn Glu Phe 
705                 710                 715                 720 


Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val Met Glu 
                725                 730                 735     


Thr Leu Arg Val Val Arg Asp Ile Trp Gln Gln Tyr Gly Gln Gly Gln 
            740                 745                 750         


Ala Ser Phe Phe Asn Glu Ile His Ile Glu Leu Gly Arg Glu Leu Lys 
        755                 760                 765             


Lys Thr Ala Lys Glu Arg Glu Lys Leu Ser Lys Gln Asn Gln Lys Asn 
    770                 775                 780                 


Glu Asp Ile Asn Leu Ser Ile Lys Glu Lys Leu Lys Glu Leu Glu Lys 
785                 790                 795                 800 


Tyr Asn Thr Arg Ala Tyr Ser Ile Thr His Gln Glu Lys Tyr Lys Leu 
                805                 810                 815     


Trp Leu Glu Gln Lys His Ile Ser Pro Tyr Thr Gly Gln Ile Ile Ser 
            820                 825                 830         


Pro Glu Asn Leu Phe Thr Asp Asn Tyr Glu Ile Glu His Ile Ile Pro 
        835                 840                 845             


Gln Ser Arg Phe Phe Asp Asp Ser Leu Ser Asn Lys Val Val Cys Glu 
    850                 855                 860                 


Ser Ile Val Asn Lys Ala Pro Tyr Lys Asp Arg Gln Leu Gly Leu Glu 
865                 870                 875                 880 


Phe Ile Lys Asn Gln Gly Gly Arg Ile Val Lys Glu Leu Ser Lys Glu 
                885                 890                 895     


Asn Lys Thr Ile Lys Ile Phe Thr Glu Glu Gln Tyr Arg Thr Phe Ile 
            900                 905                 910         


Lys Glu His Tyr Ser Asn Asn Pro Glu Lys Thr Lys Lys Leu Leu Leu 
        915                 920                 925             


Glu Glu Ile Pro Glu Lys Met Val Ala Arg Gln Met Asn Asp Thr Cys 
    930                 935                 940                 


Tyr Ile Ser Lys Phe Ile Ser Glu Ile Leu Ser Lys Ile Val Arg Ser 
945                 950                 955                 960 


Asp Glu Gln Asp Glu Gly Val His Ser Lys Asn Val Ile Leu Cys Thr 
                965                 970                 975     


Gly Lys Ile Thr Ser Ile Leu Arg Gln Asp Trp Gly Leu Asn Asp Ile 
            980                 985                 990         


Trp Asp Asn Leu Ile Leu Pro Arg  Phe Glu Arg Met Asn  Asn Leu Leu 
        995                 1000                 1005             


Gln Lys  Glu Lys Phe Thr Ile  Tyr Ser Ile Glu Lys  Gln Lys Lys 
    1010                 1015                 1020             


Ile Pro  Val Val Pro Ile Asn  Asp Gln Phe Lys Ser  Phe Gln Lys 
    1025                 1030                 1035             


Lys Arg  Ile Asp His Arg His  His Ala Met Asp Ala  Ile Ile Ile 
    1040                 1045                 1050             


Ala Cys  Thr Thr Arg Glu His  Ile Asn Tyr Ile Asn  Asn Gln His 
    1055                 1060                 1065             


Ala Leu  Glu Lys Glu Lys Asp  Lys Lys Glu Lys Gln  Glu Glu Arg 
    1070                 1075                 1080             


Asp Lys  Leu Arg Glu Lys Leu  Cys Ile Lys Lys Tyr  Asn Asn Arg 
    1085                 1090                 1095             


Ser Glu  Gly Asn Tyr Ser Trp  Ile Phe Lys Lys Pro  Trp Asp Thr 
    1100                 1105                 1110             


Phe Thr  Gln Glu Val Arg Val  Ala Leu Glu Asp Ile  Val Val Ser 
    1115                 1120                 1125             


Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys Ala Thr  Asn Tyr Tyr 
    1130                 1135                 1140             


Glu Arg  Trp Val Glu Glu Asn  Gly Lys Tyr Ile Lys  Lys Met Val 
    1145                 1150                 1155             


Lys Gln  Glu Gly Thr Asn Trp  Ala Ile Arg Lys Pro  Leu His Glu 
    1160                 1165                 1170             


Glu Thr  Ile Ser Gly Lys Ile  Tyr Leu Asp Arg Glu  Lys Ile Thr 
    1175                 1180                 1185             


Lys Asn  Asp Ile Leu Thr Ala  Thr Arg Lys Thr Val  Asp Ser Ser 
    1190                 1195                 1200             


Phe Asp  Asp Lys Arg Ile Lys  Lys Ile Thr Asp Thr  Gly Ile Gln 
    1205                 1210                 1215             


Lys Ile  Leu Leu Asn Tyr Leu  Lys Tyr Lys Gly Ser  Pro Glu Ile 
    1220                 1225                 1230             


Ala Phe  Ser Pro Glu Gly Leu  Glu Glu Leu Asn Lys  Asn Leu Ser 
    1235                 1240                 1245             


Ile Tyr  Asn Asp Gly Lys Pro  His Lys Pro Ile Tyr  Lys Val Arg 
    1250                 1255                 1260             


Ile Tyr  Glu Lys Gly Ser Gly  Arg Phe Ala Leu Gly  Glu Lys Gly 
    1265                 1270                 1275             


Arg Lys  Tyr Lys Lys Tyr Val  Gln Gly Ala Ser Asn  Leu Tyr Phe 
    1280                 1285                 1290             


Gly Ile  Tyr Gln Gly Ala Lys  Arg Ser Phe Ala Thr  Ile Pro Leu 
    1295                 1300                 1305             


Asn Glu  Val Ile Glu Arg Gln  Lys Gln Gly Leu Pro  Ser Val Pro 
    1310                 1315                 1320             


Glu Tyr  Asn Glu Lys Gly Asp  Leu Leu Leu Phe Ser  Leu Ser Pro 
    1325                 1330                 1335             


Asn Asp  Leu Val Tyr Val Pro  Ile Glu Gly Glu Ile  Ile Glu Asn 
    1340                 1345                 1350             


Ile Asp  Phe Arg Asn Leu Ser  Lys Glu Gln Lys Glu  Arg Ile Tyr 
    1355                 1360                 1365             


Asn Val  Asn Asp Phe Ser Ser  Thr Cys Tyr Phe Thr  Pro Asn Arg 
    1370                 1375                 1380             


Ile Ala  Lys Ala Ile Phe Pro  Lys Glu Val Asp Leu  Gln Arg Lys 
    1385                 1390                 1395             


Gly Asp  Lys Leu Ser Gly Ser  Tyr Asp Thr Lys Thr  Ala Ser Leu 
    1400                 1405                 1410             


Glu Gly  Ile Gln Ile Lys Glu  Val Cys Ile Lys Leu  Lys Val Asp 
    1415                 1420                 1425             


Arg Leu  Gly Asn Ile Ser Lys  Ala 
    1430                 1435     


<210>  1256
<211>  1437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1256

Met Lys Tyr Ile Lys Tyr Ile Leu Gly Leu Asp Ile Gly Ser Asn Ser 
1               5                   10                  15      


Ile Gly Trp Ala Leu Ile Gln Leu Asp Leu Leu Gln Lys Thr Lys Arg 
            20                  25                  30          


Ile Ile Ala Thr Gly Ser Arg Ile Ile Pro Met Asp Gln Lys Val Leu 
        35                  40                  45              


Ser Asp Phe Gly Lys Gly Ile Thr Ile Ser Pro Thr Ala Gln Arg Thr 
    50                  55                  60                  


Ser Tyr Arg Asn Ala Arg His Cys Arg Glu Arg His Leu Leu Arg Arg 
65                  70                  75                  80  


Glu Arg Leu His Arg Val Leu His Ile Leu Gly Phe Leu Pro Thr His 
                85                  90                  95      


Tyr Asp Lys Gln Ile Asp Phe Ser Lys Arg Tyr Gly Lys Phe Val Asp 
            100                 105                 110         


Asn Thr Glu Pro Lys Ile Ala Tyr Asn Glu Arg Lys Phe Leu Phe Met 
        115                 120                 125             


Asp Ser Phe Gln Glu Met Leu Ala Asp Phe Lys Ile His Gln Pro Asp 
    130                 135                 140                 


Leu Phe Tyr Thr Lys Lys Asn Gly Glu Glu Ser Lys Ile Pro Tyr Asp 
145                 150                 155                 160 


Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Arg Lys Ile Ser 
                165                 170                 175     


Lys Glu Glu Leu Ala Trp Ile Ile Leu Asn Phe Asn Gln Lys Arg Gly 
            180                 185                 190         


Tyr Tyr Gln Leu Arg Glu Glu Glu Glu Glu Asn Lys Glu Asn Asp Phe 
        195                 200                 205             


Glu Ile Ile Ser Ser Lys Ile Ile Ser Val Ile Lys Lys Asp Lys Asp 
    210                 215                 220                 


Lys Lys Tyr Asn Lys Tyr Trp Tyr Asp Ile Ile Leu Glu Asn Gly Leu 
225                 230                 235                 240 


Val Tyr Gln Ala Ala Phe Tyr Asn Asp Phe Val Tyr Asn Trp Ile Asn 
                245                 250                 255     


Gln Asp Arg Glu Phe Ile Ile Glu Arg Ile Ser Leu Lys Asn Gly Thr 
            260                 265                 270         


Glu Lys Val Lys Leu Ser Tyr Leu Pro Thr Phe Asp Glu Ile Asp Lys 
        275                 280                 285             


Met Asp Lys Lys Glu Gln Asp Lys Tyr Tyr Lys Lys Ile Lys Ile Lys 
    290                 295                 300                 


Thr Glu Asn Ser Ile Gly Asn Lys Thr Val Gly Cys Tyr Ile Tyr Asp 
305                 310                 315                 320 


His Ile Leu Gln Asn Pro Asn Glu Lys Ile Lys Gly Lys Leu Val Arg 
                325                 330                 335     


Thr Ile Glu Arg Lys Tyr Tyr Lys Glu Glu Leu Lys Ala Ile Leu Glu 
            340                 345                 350         


Lys Gln Val Ala Leu Gln Pro Glu Leu Phe Thr Asp Gln Leu Phe Ala 
        355                 360                 365             


Asp Cys Ile Arg Glu Leu Tyr Ser Lys Asn Glu Ala Gln Gln Arg Asn 
    370                 375                 380                 


Leu Ala Ala Arg Asp Phe Val His Leu Phe Val Glu Asp Ile Ile Phe 
385                 390                 395                 400 


Tyr Gln Arg Ser Leu Arg Arg Gln Lys Ser Thr Leu Ala Asn Cys Ser 
                405                 410                 415     


Leu Glu Ser Arg Ser Tyr Ile Asp Lys Asp Ser His Thr Arg Lys Glu 
            420                 425                 430         


Ala Pro Leu Lys Val Cys Pro Lys Ser Asn Pro Tyr Tyr Gln Glu Phe 
        435                 440                 445             


Arg Val Leu Gln Trp Leu Gln Asn Leu Lys Ile Tyr Glu Ile Asp Thr 
    450                 455                 460                 


Asp Gln Glu Val Thr His Glu Phe Ile Lys Thr Leu Glu Asp Lys Gln 
465                 470                 475                 480 


Gln Phe Phe Asp Phe Leu Met Asn Gln Lys Glu Ile Asp Cys Glu Glu 
                485                 490                 495     


Leu Leu Lys Tyr Phe Leu Asn Gln Thr Tyr Pro Asn Ala Arg Glu Lys 
            500                 505                 510         


Ala Leu Lys Ser Glu Leu Lys Lys Trp Lys Asp Thr Tyr Arg Trp Asn 
        515                 520                 525             


Tyr Val Tyr Asp Ile Gly Glu Lys Ser Ser Lys Lys Tyr Pro Met Asn 
    530                 535                 540                 


Glu Thr Arg Tyr Glu Leu Lys Arg His Leu Glu Lys Val Ala Asn Leu 
545                 550                 555                 560 


Pro Asp Asp Phe Leu Ser Ser Glu Val Glu Tyr Leu Leu Trp His Leu 
                565                 570                 575     


Ile Tyr Ser Val Lys Asp Lys Val Ala Tyr Glu Lys Gly Leu Lys Thr 
            580                 585                 590         


Phe Ala Gln Lys His His Leu Asp Lys Asp Ser Phe Val Glu Ser Phe 
        595                 600                 605             


Lys Lys Phe Lys Pro Tyr Pro Ser Glu Tyr Gly Ser Phe Ser Glu Lys 
    610                 615                 620                 


Ala Ile Arg Lys Leu Leu Pro Leu Met Arg Phe Gly Ser Tyr Trp Asp 
625                 630                 635                 640 


Phe Asn His Ile Asp Lys Asn Thr Gln Lys Arg Ile Glu Asp Leu Ile 
                645                 650                 655     


Thr Gly Val Glu Asn Glu Glu Ile Arg Thr Ile Leu Arg Glu Lys Ala 
            660                 665                 670         


Glu Lys Tyr Gln Leu Glu Lys Glu Thr Asp Phe Gln Asp Leu Pro Leu 
        675                 680                 685             


Trp Leu Ala Gln Tyr Ile Val Tyr Asn Arg His Ala Glu Ala Ser Ser 
    690                 695                 700                 


Leu Glu Lys Trp Thr Ser Val Asn Asp Leu Glu Thr Tyr Leu Asn Glu 
705                 710                 715                 720 


Phe Lys Gln Tyr Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val Met 
                725                 730                 735     


Glu Thr Leu Arg Val Val Arg Asp Ile Trp Gln Gln Tyr Gly Gln Gly 
            740                 745                 750         


Gln Ala Ser Phe Phe Asp Glu Ile His Ile Glu Leu Gly Arg Glu Leu 
        755                 760                 765             


Lys Lys Thr Ala Lys Glu Arg Glu Lys Leu Ser Lys Gln Asn Gln Lys 
    770                 775                 780                 


Asn Glu Asp Ile Asn Leu Ser Ile Lys Glu Lys Leu Lys Glu Leu Glu 
785                 790                 795                 800 


Lys Tyr Asn Thr Arg Ala Tyr Ser Ile Thr His Gln Glu Lys Tyr Lys 
                805                 810                 815     


Leu Trp Leu Glu Gln Lys His Ile Ser Pro Tyr Thr Gly Gln Ile Ile 
            820                 825                 830         


Ser Pro Glu Asn Leu Phe Thr Asp Asn Tyr Glu Ile Glu His Ile Ile 
        835                 840                 845             


Pro Gln Ser Arg Phe Phe Asp Asp Ser Leu Ser Asn Lys Val Val Cys 
    850                 855                 860                 


Glu Ser Ile Val Asn Lys Ala Pro Tyr Lys Asp Arg Gln Leu Gly Leu 
865                 870                 875                 880 


Glu Phe Ile Lys Asn Gln Gly Gly Arg Ile Val Lys Glu Leu Ser Thr 
                885                 890                 895     


Glu Asn Lys Thr Ile Lys Ile Phe Thr Glu Glu Gln Tyr Arg Thr Phe 
            900                 905                 910         


Ile Lys Glu His Tyr Ser Asn Asn Pro Glu Lys Thr Lys Lys Leu Leu 
        915                 920                 925             


Leu Glu Glu Ile Pro Glu Lys Met Val Ala Arg Gln Met Asn Asp Thr 
    930                 935                 940                 


Cys Tyr Ile Ser Lys Phe Ile Ser Glu Ile Leu Ser Lys Ile Val Arg 
945                 950                 955                 960 


Ser Asp Glu Gln Asp Glu Gly Val His Ser Lys Asn Val Ile Leu Cys 
                965                 970                 975     


Thr Gly Lys Ile Thr Ser Ala Leu Lys Gln Asp Trp Gly Val Asn Asp 
            980                 985                 990         


Val Trp Asn Asp Leu Ile Leu Pro  Arg Phe Glu Arg Met  Asn Asp Leu 
        995                 1000                 1005             


Thr Gln  Ser Thr Leu Phe Thr  Thr Tyr Asn Glu Lys  Tyr Gln Lys 
    1010                 1015                 1020             


Tyr Leu  Pro Thr Val Pro Met  Glu Tyr Ser Asn Gly  Phe Gln Lys 
    1025                 1030                 1035             


Lys Arg  Ile Asp His Arg His  His Ala Met Asp Ala  Ile Ile Ile 
    1040                 1045                 1050             


Ala Cys  Ala Thr Arg Glu His  Ile Asn Tyr Ile Asn  Asn Gln His 
    1055                 1060                 1065             


Ala Leu  Glu Lys Gly Lys Asp  Lys Lys Glu Lys Gln  Lys Glu His 
    1070                 1075                 1080             


Asp Lys  Leu Arg Glu Lys Leu  Cys Ile Lys Lys Asp  Asn Lys Gly 
    1085                 1090                 1095             


Ser Glu  Glu Asn Tyr Ser Trp  Ile Phe Lys Lys Pro  Trp Asp Thr 
    1100                 1105                 1110             


Phe Thr  Gln Glu Val Gln Ala  Ala Leu Glu Gly Ile  Val Val Ser 
    1115                 1120                 1125             


Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys Ala Thr  Asn Tyr Tyr 
    1130                 1135                 1140             


Glu Arg  Trp Val Glu Glu Asn  Gly Lys Tyr Ile Lys  Lys Met Val 
    1145                 1150                 1155             


Lys Gln  Glu Gly Thr Asn Trp  Ala Ile Arg Lys Pro  Leu His Glu 
    1160                 1165                 1170             


Glu Thr  Ile Ser Gly Lys Ile  Tyr Leu Asp Arg Glu  Glu Ile Thr 
    1175                 1180                 1185             


Lys Asn  Asp Ile Leu Thr Ala  Thr Arg Lys Thr Val  Asp Ser Ser 
    1190                 1195                 1200             


Phe Asp  Glu Lys Arg Ile Lys  Lys Ile Thr Asp Thr  Gly Ile Gln 
    1205                 1210                 1215             


Lys Ile  Leu Leu Asn Tyr Leu  Lys Tyr Lys Gly Ser  Pro Glu Ile 
    1220                 1225                 1230             


Ala Phe  Ser Pro Glu Gly Leu  Glu Glu Leu Asn Lys  Asn Leu Ser 
    1235                 1240                 1245             


Ile Tyr  Asn Asp Gly Lys Pro  His Lys Pro Ile Tyr  Lys Val Arg 
    1250                 1255                 1260             


Ile Tyr  Glu Lys Gly Ser Gly  Arg Phe Ala Leu Gly  Glu Lys Gly 
    1265                 1270                 1275             


Arg Lys  Tyr Lys Lys Tyr Val  Gln Gly Ala Pro Asn  Leu Tyr Phe 
    1280                 1285                 1290             


Gly Ile  Tyr Gln Gly Ala Asn  Lys Arg Ser Phe Ala  Thr Ile Pro 
    1295                 1300                 1305             


Leu Asn  Glu Val Ile Glu Arg  Gln Lys Gln Gly Arg  Pro Ser Ile 
    1310                 1315                 1320             


Pro Glu  Tyr Asn Glu Lys Gly  Asp Pro Leu Leu Phe  Ser Leu Ser 
    1325                 1330                 1335             


Pro Asn  Asp Leu Val Tyr Val  Pro Val Glu Gly Glu  Ile Ile Glu 
    1340                 1345                 1350             


Asp Ile  Asp Phe Gln Asn Leu  Ser Lys Glu Gln Lys  Glu Arg Ile 
    1355                 1360                 1365             


Tyr Asn  Val Asn Asp Phe Ser  Ser Thr Cys Tyr Phe  Thr Pro Asn 
    1370                 1375                 1380             


Arg Ile  Ala Lys Ala Ile Phe  Pro Lys Glu Val Asp  Leu Gln Arg 
    1385                 1390                 1395             


Lys Gly  Asp Lys Leu Pro Gly  Ser Tyr Asp Thr Lys  Thr Ala Ser 
    1400                 1405                 1410             


Leu Glu  Gly Ile Gln Ile Lys  Glu Val Cys Ile Lys  Leu Lys Ile 
    1415                 1420                 1425             


Asp Arg  Leu Gly Asn Ile Ser  Lys Ala 
    1430                 1435         


<210>  1257
<211>  1438
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1257

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Lys Gln Asn Phe Glu Asn Lys Tyr Gly Glu Ile Leu Gly 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Glu Phe 
        35                  40                  45              


Gly Lys Gly Asn Ser Val Ser Gln Thr Ala Ala Arg Thr Asp Tyr Arg 
    50                  55                  60                  


Ala Thr Arg Arg Leu Arg Glu Arg Phe Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Asn Val Leu Asn Phe Leu Pro Glu His Tyr Ala Ser 
                85                  90                  95      


Gln Ile Asp Phe Asp Lys Arg Phe Gly Lys Phe Lys Val Glu Thr Glu 
            100                 105                 110         


Pro Lys Leu Ala Trp Lys Asn Ile Asp Gly Lys Phe Ser Phe Leu Phe 
        115                 120                 125             


Gln Thr Ser Phe Asn Glu Met Leu Glu Asp Phe Lys Ala His Gly Gln 
    130                 135                 140                 


Asp Leu Lys Val Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys 
145                 150                 155                 160 


Ala Leu Ser Gln Lys Ile Glu Lys Glu Glu Leu Val Trp Ile Leu Leu 
                165                 170                 175     


Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu 
            180                 185                 190         


Glu Glu Asn Pro Asn Lys Leu Ile Glu Phe Tyr Ser Leu Lys Ile Ile 
        195                 200                 205             


Asp Val Leu Ala Asp Glu Pro Gln Lys Gly Lys Ser Asp Ile Trp Tyr 
    210                 215                 220                 


Ser Leu Val Leu Glu Asn Gly Trp Ile Tyr Arg Arg Ser Ser Lys Thr 
225                 230                 235                 240 


Ser Leu Leu Asp Trp Lys Asp Lys Ile Arg Asp Phe Ile Val Thr Thr 
                245                 250                 255     


Asp Leu Asn Asp Asp Gly Ser Val Lys Thr Asp Lys Glu Gly Asn Glu 
            260                 265                 270         


Lys Arg Ser Phe Arg Ala Pro Gly Glu Asn Asp Trp Thr Leu Val Lys 
        275                 280                 285             


Thr Lys Thr Glu Gln Glu Ile Asp Arg Ser Arg Lys Thr Val Gly Asn 
    290                 295                 300                 


Tyr Ile Tyr Glu Thr Leu Leu Gln Asn Pro Lys Gln Lys Ile Lys Gly 
305                 310                 315                 320 


Lys Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Glu Glu Leu Lys 
                325                 330                 335     


Arg Ile Leu Glu Lys Gln Lys Glu Phe His Arg Glu Leu Glu Ser Asn 
            340                 345                 350         


Asp Leu Tyr Asn Asp Cys Ile Arg Glu Leu Tyr Arg Asn Asn Glu Ala 
        355                 360                 365             


His Gln Leu Thr Leu Ser Lys Lys Asp Phe Val His Leu Phe Met Glu 
    370                 375                 380                 


Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Gln Lys Ser Ser Ile 
385                 390                 395                 400 


Ser Asn Cys Thr Leu Glu Phe Arg Lys Tyr Lys Asp Glu Asn Gly Ile 
                405                 410                 415     


Glu His Thr Gln Phe Leu Lys Ala Ile Pro Lys Ser Asn Pro Tyr Tyr 
            420                 425                 430         


Gln Glu Phe Arg Ile Trp Gln Trp Ile Phe Asn Leu Asn Ile Tyr Lys 
        435                 440                 445             


Lys Asp Asn Asp Asp Asn Val Thr Lys Glu Phe Leu Asn Thr Thr Arg 
    450                 455                 460                 


Asp Phe Glu Asn Leu Phe Glu Phe Leu Asn Asn Arg Lys Glu Ile Glu 
465                 470                 475                 480 


Gln Lys Pro Leu Leu Lys Phe Leu Leu Glu Gln Lys Glu Phe Lys Gly 
                485                 490                 495     


Lys Leu Leu Asn Ala Glu Thr Glu Lys Phe Arg Trp Asn Tyr Val Glu 
            500                 505                 510         


Asp Lys Lys Tyr Pro Cys Asn Glu Thr Arg Thr Met Ile Ser Glu Arg 
        515                 520                 525             


Leu Lys Lys Val Glu Asn Ile Ser Asp Asp Phe Leu Thr Arg Glu Met 
    530                 535                 540                 


Glu Gln Lys Ile Trp His Ile Ile Tyr Ser Val Asn Asp Lys Thr Glu 
545                 550                 555                 560 


Tyr Glu Lys Ala Leu Lys Ser Phe Ala Glu Lys Asn Asn Leu Asp Glu 
                565                 570                 575     


Asn Ser Leu Phe Glu Ala Phe Lys Lys Leu Pro Pro Phe Lys Ser Glu 
            580                 585                 590         


Tyr Gly Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu Met 
        595                 600                 605             


Arg Leu Gly Lys Tyr Trp Ser Tyr Ala Lys Ile Asp Leu Tyr Ser Lys 
    610                 615                 620                 


Asn Arg Ile Gln Lys Ile Ile Thr Gly Glu Phe Asp Glu Asn Ile Lys 
625                 630                 635                 640 


Asp Arg Val Arg Glu Lys Ala Ile Arg Leu Thr Ala Glu Asn Asp Phe 
                645                 650                 655     


Gln Gly Leu Gln Leu Trp Leu Ala Gln Tyr Ile Val Tyr Gly Arg His 
            660                 665                 670         


Ser Glu Ala Ser Met Ile Gly Lys Trp Asn Ser Ala Asp Asp Leu Glu 
        675                 680                 685             


Glu Phe Leu Lys Glu Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val 
    690                 695                 700                 


Glu Gln Val Ile Thr Glu Thr Leu Arg Ile Val Lys Asp Ile Trp Leu 
705                 710                 715                 720 


Lys Tyr Gly Asn Gly Ala Lys Asp Phe Phe Asn Glu Ile His Ile Glu 
                725                 730                 735     


Leu Gly Arg Glu Met Lys Gln Thr Lys Asp Glu Arg Ala Asn Ala Thr 
            740                 745                 750         


Lys Thr Ile Thr Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala Leu 
        755                 760                 765             


Leu Ala Glu Met Met Asn Asp His Ser Val Glu Asn Val Arg Pro Tyr 
    770                 775                 780                 


Ser Pro Met Gln Gln Glu Ile Leu Lys Ile Tyr Glu Asp Gly Ile Leu 
785                 790                 795                 800 


Lys Ser Asp Ile Glu Ile Asp Asp Asp Ile Leu Lys Ile Ser Lys Thr 
                805                 810                 815     


Ala Gln Pro Ser Ser Ser Asp Leu Lys Arg Tyr Lys Leu Trp Leu Glu 
            820                 825                 830         


Gln Lys Tyr Lys Ser Pro Tyr Thr Gly Gln Ile Ile Pro Leu Asn Lys 
        835                 840                 845             


Leu Phe Thr Pro Glu Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg 
    850                 855                 860                 


Tyr Phe Asp Asp Ser Phe Ser Asn Lys Ile Ile Cys Glu Ser Ala Val 
865                 870                 875                 880 


Asn Lys Leu Lys Asp Asn Tyr Ile Gly Leu Gly Phe Ile Lys Gln Phe 
                885                 890                 895     


Gly Gly Thr Ile Ile Glu Cys Gly Phe Gly Lys Arg Val Lys Val Phe 
            900                 905                 910         


Glu Val Gly Glu Tyr Glu Glu Phe Val Lys Lys His Tyr Ala Asn Asn 
        915                 920                 925             


Arg Gly Lys Arg Asn Lys Leu Leu Leu Glu Glu Ile Pro Glu Lys Met 
    930                 935                 940                 


Ile Glu Arg Gln Met Asn Asp Thr Arg His Ile Ser Lys Tyr Ile Ser 
945                 950                 955                 960 


Gly Ile Leu Ser His Ile Val Arg Val Glu Asp Gly Thr Asp Glu Gly 
                965                 970                 975     


Val Asn Ser Lys Asn Val Ile Pro Gly Asn Gly Lys Ile Thr Thr Gln 
            980                 985                 990         


Leu Arg Gln Asp Trp Gly Leu Asn  Asp Val Trp Asn Glu  Leu Ile Leu 
        995                 1000                 1005             


Pro Arg  Phe Glu Arg Met Asn  Gln Leu Thr Asn Ser  Thr Asp Phe 
    1010                 1015                 1020             


Thr Ser  Trp Asn Glu Asn His  Gln Lys Tyr Leu Pro  Thr Val Pro 
    1025                 1030                 1035             


Val Glu  Phe Ser Lys Gly Phe  Ser Lys Lys Arg Ile  Asp His Arg 
    1040                 1045                 1050             


His His  Ala Leu Asp Ala Leu  Val Ile Ala Cys Ala  Thr Lys Asp 
    1055                 1060                 1065             


His Val  Asn Leu Leu Asn Asn  Gln Ser Ala Lys Ser  Ala Thr Lys 
    1070                 1075                 1080             


Arg Tyr  Asp Leu Lys Lys Lys  Leu Met Lys Phe Glu  Lys Thr Val 
    1085                 1090                 1095             


Tyr Lys  Asp Pro Gln Thr Glu  Lys Arg Ile Glu Arg  Glu Val Pro 
    1100                 1105                 1110             


Lys His  Phe Leu Lys Pro Trp  Glu Thr Phe Thr Val  Asp Ala Lys 
    1115                 1120                 1125             


Asn Arg  Leu Glu Thr Ile Ile  Val Ser Phe Lys Gln  Asn Leu Arg 
    1130                 1135                 1140             


Val Ile  Asn Lys Ala Thr Asn  Tyr Tyr Glu Lys Tyr  Val Glu Lys 
    1145                 1150                 1155             


Asp Gly  Val Met Met Lys Glu  Arg Val Glu Gln Thr  Gly Thr Asn 
    1160                 1165                 1170             


Trp Ala  Ile Arg Lys Pro Met  His Lys Glu Thr Val  Ser Gly Lys 
    1175                 1180                 1185             


Ile Asp  Leu Pro Trp Val Lys  Val Pro Lys Gly Lys  Ile Leu Thr 
    1190                 1195                 1200             


Ala Thr  Arg Lys Ser Leu Asp  Thr Ser Phe Asp Leu  Lys Ala Ile 
    1205                 1210                 1215             


Ala Ser  Ile Thr Asp Thr Gly  Ile Gln Lys Ile Leu  Lys Asn Tyr 
    1220                 1225                 1230             


Leu Glu  Phe Lys Glu Ser Pro  Glu Leu Ala Phe Ser  Pro Glu Gly 
    1235                 1240                 1245             


Ile Glu  Asp Met Asn Lys Asn  Ile Glu Lys Tyr Asn  Gly Gly Arg 
    1250                 1255                 1260             


Pro His  Gln Pro Ile Ser Lys  Val Arg Val Phe Glu  Leu Gly Ser 
    1265                 1270                 1275             


Lys Phe  Gln Val Gly Gln Thr  Gly Asn Lys Lys Asp  Lys Tyr Val 
    1280                 1285                 1290             


Glu Ala  Ala Lys Gly Thr Asn  Leu Phe Phe Ala Ile  Tyr Glu Asp 
    1295                 1300                 1305             


Lys Lys  Gly Lys Arg Ser Tyr  Glu Thr Ile Pro Leu  Asn Glu Val 
    1310                 1315                 1320             


Ile Glu  Arg Gln Lys Gln Gly  Leu Ser Val Val Asp  Leu Lys Asn 
    1325                 1330                 1335             


Val Asn  Asp Phe Phe Leu Cys  Pro Asn Asp Leu Val  Tyr Ile Pro 
    1340                 1345                 1350             


Ser Gly  Asp Glu Phe Glu Asn  Arg Gly Ser Ile Glu  Ile Glu Asn 
    1355                 1360                 1365             


Ile Thr  Lys Glu Lys Ser Glu  Arg Phe Tyr Lys Val  Val Ser Phe 
    1370                 1375                 1380             


Ser Gly  Ser Gln Ile Phe Phe  Val Arg His Asp Ile  Ala Val Ser 
    1385                 1390                 1395             


Ile Val  Asn Lys Gly Glu Phe  Ser Thr Leu Asn Lys  Met Glu Arg 
    1400                 1405                 1410             


Ala Ile  Asp Gly Ser Met Val  Lys Glu Ile Cys Ile  Lys Leu Lys 
    1415                 1420                 1425             


Ile Asp  Arg Leu Gly Asp Val  Leu Lys Ala 
    1430                 1435             


<210>  1258
<211>  1438
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1258

Met Val Lys Lys Gly Tyr Thr Ile Gly Leu Asp Ile Gly Thr Thr Ser 
1               5                   10                  15      


Val Gly Trp Ala Val Thr Asp Asp Asp Phe Glu Leu Val Asn Lys Lys 
            20                  25                  30          


Met Ser Ile Lys Asp Lys Ser Gly Ser Thr Ile Lys Arg Val Arg Lys 
        35                  40                  45              


Asn Leu Trp Gly Val Arg Leu Phe Asp Glu Ala Lys Thr Ala Glu Asn 
    50                  55                  60                  


Thr Arg Arg Thr Arg Gly Val Arg Arg Arg Ile Arg Arg Arg Lys Glu 
65                  70                  75                  80  


Arg Leu Asn Tyr Leu Gln Gly Ile Phe Ser Ser Glu Met Ala Lys Met 
                85                  90                  95      


Asp Glu Asn Phe Phe Met Arg Leu Glu Glu Ser Phe Phe Lys Leu Ser 
            100                 105                 110         


Asp Lys Ser Glu Gln Asn Lys Phe Pro Tyr Ala Leu Phe Lys Asn Glu 
        115                 120                 125             


Ile Glu Glu Lys Ala Tyr Tyr Arg Gln Phe Pro Thr Ile Tyr His Leu 
    130                 135                 140                 


Arg Lys His Leu Met Glu His Glu Glu Ser Asp Leu Arg Lys Ile Tyr 
145                 150                 155                 160 


Leu Gly Leu His His Ile Leu Lys Tyr Arg Gly His Phe Val Gln Glu 
                165                 170                 175     


Gly Gln Lys Met Asp Val Ala Asn Ile Asp Ile Gln Gly Asn Leu Lys 
            180                 185                 190         


Glu Leu Phe Asn Ala Leu Asn Glu Asn Phe Ser Asn Lys Glu Asp Asp 
        195                 200                 205             


Phe Glu Gln Glu Arg Met Lys Asp Gly Val Lys Arg Ala Lys Val Leu 
    210                 215                 220                 


Phe Asp Thr Glu Lys Leu Ala Ala Val Glu Glu Ile Leu Lys Glu Lys 
225                 230                 235                 240 


Ile Ser Lys Ser Trp Lys Val Asn Lys Ile Ser Lys Gly Ile Leu Ala 
                245                 250                 255     


Glu Lys Lys Asp Leu Pro Ile Phe Lys Ala Ile Val Gly Asn Lys Ile 
            260                 265                 270         


Asp Leu Ala Gly Ile Phe Asp Cys Pro Asp Tyr Ala Glu Lys Val Asn 
        275                 280                 285             


Glu Ser Ile Lys Glu Leu Lys Asp Lys Val Tyr Phe Asn Lys Glu Asp 
    290                 295                 300                 


Phe Glu Glu Thr Phe Glu Lys Leu Ser Gln Leu Leu Lys Glu Glu Glu 
305                 310                 315                 320 


Leu Glu Ile Leu Ile Leu Ala Arg Lys Cys Tyr Glu Ala Val Leu Leu 
                325                 330                 335     


Ala Asp Ile Met Gln Gly Glu Gln Ser Leu Ser Asp Ala Met Val Ala 
            340                 345                 350         


Lys Tyr Asn Asp His Lys Lys Asp Leu Val Ile Phe Lys Lys Phe Val 
        355                 360                 365             


Ile Asp Tyr Phe Ser Glu Asp Val Tyr Phe Asp Leu Phe Lys Asn Thr 
    370                 375                 380                 


Gln Leu Ala Gly Asn Tyr His Ser Tyr Val Lys Gly Glu Arg Lys Gly 
385                 390                 395                 400 


Asn Lys Phe Asn His Ala Ser Leu Asp Asp Phe Tyr Lys Phe Val Lys 
                405                 410                 415     


Lys Glu Leu Asp Lys Lys Ala Glu Glu Leu Ser Glu His Pro Val Tyr 
            420                 425                 430         


Lys Gln Ile Leu Gln Lys Ile Glu Leu Glu Thr Phe Leu Pro Lys Gln 
        435                 440                 445             


Arg Met Phe Lys Asn Gly Ala Ile Pro Tyr Gln Val His Leu His Glu 
    450                 455                 460                 


Leu Arg Ala Ile Ile Lys Lys Gln Glu Lys Arg Phe Pro Phe Leu Ala 
465                 470                 475                 480 


Glu Lys Ile Ile Ile Lys Asp Asn Gln Lys Asp Ile Ser Glu Tyr Lys 
                485                 490                 495     


Ile Glu Thr Leu Met Lys Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu 
            500                 505                 510         


Ile Glu Ala Arg Glu Gly Glu Leu Gly Lys Lys Arg Thr Asn Tyr Ser 
        515                 520                 525             


Arg Phe Ala Trp Met Gln Lys Ser Pro Glu Ser Gln Asn Ile Gly Ile 
    530                 535                 540                 


Thr Pro Trp Asn Phe His Ser Val Val Asp Lys Asp Ala Ser Ala Ser 
545                 550                 555                 560 


Glu Phe Ile Glu Arg Met Thr Gly Phe Cys Ser Tyr Phe Pro Glu Glu 
                565                 570                 575     


Pro Val Leu Pro Lys Asn Ser Leu Ile Tyr Gln Gln Phe Thr Val Phe 
            580                 585                 590         


Gln Glu Leu Ser Ile Val Gly Tyr Tyr Asp Glu Gln Asn Arg Lys His 
        595                 600                 605             


Tyr Leu Asp Gly Glu Thr Arg Arg Arg Ile Phe Glu Asp Leu Phe Lys 
    610                 615                 620                 


Lys Asn Arg Thr Val Ser Ala Gly Leu Leu Arg Lys Trp Leu Val Cys 
625                 630                 635                 640 


Asn Gln Arg Thr Ser Gln Glu Glu Lys Glu Ile Arg Leu Phe Gly Leu 
                645                 650                 655     


Asp Leu Lys Lys Asn Gly Leu Gly Ser Gly Asn Phe Asn Thr Lys Tyr 
            660                 665                 670         


Thr Thr Tyr Leu Asp Leu Lys Asp Ala Gly Ile Thr Glu Lys Gln Leu 
        675                 680                 685             


Lys Gln His Lys Asp Phe Phe Glu Lys Ile Val Lys Tyr Gln Thr Ile 
    690                 695                 700                 


Phe Glu Asp Lys Lys Ile Leu Lys Arg Arg Ile Ile Leu Leu Asn Glu 
705                 710                 715                 720 


Glu Gln Pro Leu Leu Ser Glu Glu Gln Ile Lys Asn Leu Ala Lys Lys 
                725                 730                 735     


His Tyr Thr Gly Trp Gly Asn Leu Ser Leu Lys Leu Ile Asp Gly Ile 
            740                 745                 750         


Gln Asp Lys Gly Lys Thr Ile Leu Asp Tyr Leu Glu Thr Gln Asp Tyr 
        755                 760                 765             


Asn Gln Asn Leu Met Gln Leu Met Asn Asp Glu Asn Lys Ser Phe Glu 
    770                 775                 780                 


Met Gln Ile Glu Gln Ala Arg Ala Gly Lys Val Gln Phe Gly Lys Ile 
785                 790                 795                 800 


Asp Tyr Ser Phe Val Glu Gln Leu Ala Gly Ser Pro Ala Ile Lys Arg 
                805                 810                 815     


Gly Ile Trp Gln Ser Leu Gln Ile Val Glu Glu Leu Val Ala Phe Leu 
            820                 825                 830         


Gly Lys Glu Asn Ile Lys Lys Ile Val Ile Glu Met Ala Arg Glu Asn 
        835                 840                 845             


Gln Ser Ser Lys Arg Thr Lys Ser Arg Gln Lys Gln Ile Glu Ala Leu 
    850                 855                 860                 


Tyr Gln Lys Val Thr Gly Asp Leu Lys Asn Glu Gln Glu Lys Ile Asp 
865                 870                 875                 880 


Val Met Gln Asn Phe Asp Asn Glu Arg Val Phe Leu Tyr Tyr Leu Gln 
                885                 890                 895     


Asn Gly Lys Cys Met His Ser Gly Glu Lys Leu Glu Leu Ser Gln Leu 
            900                 905                 910         


Ser Glu Tyr Glu Ile Asp His Ile Ile Pro Gln Thr Tyr Ile Lys Asp 
        915                 920                 925             


Asp Ser Phe Asp Asn Lys Val Leu Val Thr Arg Asn Ala Asn Gln Gln 
    930                 935                 940                 


Lys Gly Gly Asp Val Pro Ser Tyr Asp Val Ile Asp Lys Met Lys Ser 
945                 950                 955                 960 


Tyr Trp Glu Lys Leu Lys Glu Asn Asn Leu Ile Ser Glu Lys Lys Phe 
                965                 970                 975     


Ala Asn Leu Thr Lys Gly Lys Phe Asp Asp Gln Gln Leu Ser Gly Phe 
            980                 985                 990         


Ile Asn Arg Gln Leu Val Glu Thr  Arg Gln Ile Thr Lys  Asn Val Ala 
        995                 1000                 1005             


Ile Ile  Leu Asp Lys Ile Tyr  Asn Asp Glu Ala Lys  Asn Glu Lys 
    1010                 1015                 1020             


Glu Val  Ala Ile Ile Thr Leu  Lys Ser Ala Leu Thr  Ser Gln Tyr 
    1025                 1030                 1035             


Arg Gln  Gly Ile Ile Tyr Val  Pro Asn Asp Asp Phe  Asp Glu Leu 
    1040                 1045                 1050             


Lys Leu  Val Ser Lys Thr Asn  Ser Arg Leu Lys Glu  Val Lys Leu 
    1055                 1060                 1065             


His Asp  Gly Phe Tyr Lys Val  Arg Glu Ile Asn Asp  Tyr His His 
    1070                 1075                 1080             


Ala His  Asp Ala Tyr Leu Asn  Asn Val Val Ala Thr  Tyr Leu Tyr 
    1085                 1090                 1095             


Glu Ala  Met Pro Asp Leu Arg  Pro Leu Phe Val Tyr  Gly Glu His 
    1100                 1105                 1110             


Ser Arg  Glu Ile His Lys Arg  Phe Leu Gly Lys Tyr  Ala Thr Thr 
    1115                 1120                 1125             


Arg Lys  Gly Gln Phe Lys Gln  Leu Leu Val Asp Met  Gln Asp Glu 
    1130                 1135                 1140             


Tyr Trp  Met Arg Lys Asp Lys  Glu Gly Asn Phe Ile  Asp Gly Glu 
    1145                 1150                 1155             


Ile Leu  Trp Arg Arg Asp Glu  Val Leu Gln Ile Val  Lys Lys Val 
    1160                 1165                 1170             


Leu Asp  Tyr Arg Asn Ile Gln  Ile Thr Lys Lys Leu  Glu Met Met 
    1175                 1180                 1185             


Val Thr  Gly Gly Glu Glu Lys  Ser Phe Tyr Gln Asp  Ser Arg Cys 
    1190                 1195                 1200             


Arg Lys  Asp Lys Thr Ala Ile  Pro Tyr Lys Thr Asn  Trp Asp Pro 
    1205                 1210                 1215             


Ser Lys  Tyr Gly Gly Phe Lys  Ser Pro Arg Thr Ala  Tyr Ala Ile 
    1220                 1225                 1230             


Pro Val  Ile Lys Lys Asn Lys  Pro Lys Leu Glu Pro  Val Leu Leu 
    1235                 1240                 1245             


Val Asn  Arg Ser Lys Val Glu  Thr Asp Gly Gln Phe  Asp Cys Glu 
    1250                 1255                 1260             


Lys Phe  Lys Glu Leu Asn Pro  Thr Leu Lys Ile Leu  Gly Gln Pro 
    1265                 1270                 1275             


Ile Trp  Lys Tyr Gln Ser Phe  Glu Met Phe Asp Gly  Thr Lys Arg 
    1280                 1285                 1290             


Tyr Ile  Ala Ser Ala Ser Glu  Ile His Lys Gly Asn  Gln Ile Lys 
    1295                 1300                 1305             


Phe Ser  Gln Glu His Met Lys  Phe Ile Ser Cys Cys  Gln Lys Tyr 
    1310                 1315                 1320             


Asp Glu  Val Lys Asn Lys Leu  Ala Phe Asp Phe Val  Asn Glu Ser 
    1325                 1330                 1335             


Lys His  Leu Phe Gln Glu Ile  Leu Thr Ala Ile Lys  Asp Asn Ile 
    1340                 1345                 1350             


His Arg  Tyr Arg Leu Ile Ala  Glu Gly Ile Glu Val  His Arg Lys 
    1355                 1360                 1365             


Phe Ser  Asn Met Leu Glu Asn  Ile Glu Lys Leu Ser  Thr Gln Glu 
    1370                 1375                 1380             


Leu Val  Asp Asn Leu Leu Asp  Ala Ile Asn Ile Thr  Ser Val Gly 
    1385                 1390                 1395             


Thr Thr  Asn Asn Ala Phe Gly  Arg Val Arg Tyr Lys  Gly Ser Tyr 
    1400                 1405                 1410             


Leu Asn  Glu Ile Trp Ser Ala  Thr Leu Ile His Gln  Ser Val Thr 
    1415                 1420                 1425             


Gly Leu  Tyr Glu Thr Arg Glu  Lys Leu Ile 
    1430                 1435             


<210>  1259
<211>  1440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1259

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Glu Gln Asp Phe Glu Asn Lys Gln Gly Leu Ile Leu Gly 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Met Asp Ala Gly Ile Ile Gly Lys Phe 
        35                  40                  45              


Ala Glu Gly Ser Ser Ile Ser Gln Thr Ala Glu Arg Thr Gly Tyr Arg 
    50                  55                  60                  


Ser Ile Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Asn Ile Leu Lys Phe Leu Pro Glu His Tyr Ala Ala 
                85                  90                  95      


Glu Ile Asp Phe Glu Lys Arg Phe Gly Gln Phe Leu Ala Glu Thr Glu 
            100                 105                 110         


Pro Lys Leu Ala Trp Lys Lys Ser Ala Glu Gly Lys Phe Glu Phe Leu 
        115                 120                 125             


Phe Gln Arg Ser Phe Asp Glu Met Val Ala Asp Phe Lys Ala Asn Gly 
    130                 135                 140                 


Gln Asp Ile Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys 
145                 150                 155                 160 


Lys Ala Leu Thr Gln Lys Ile Ser Pro Gln Glu Leu Ala Trp Ile Ile 
                165                 170                 175     


Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu 
            180                 185                 190         


Glu Glu Glu Asn Pro Asn Lys Lys Val Glu Phe Tyr Ser Leu Lys Ile 
        195                 200                 205             


Val Asp Val Val Ala Asp Glu Gln Pro Asn Lys Lys Gly Asp Thr Trp 
    210                 215                 220                 


Tyr Ser Leu His Leu Glu Asn Gly Trp Val Tyr Arg Arg Ser Ser Lys 
225                 230                 235                 240 


Ile Pro Leu Tyr Asp Trp Lys Asp Lys Val Arg Asp Phe Ile Val Thr 
                245                 250                 255     


Thr Asp Ile Asn Glu Asp Gly Ser Glu Lys Leu Asp Lys Asp Gly Glu 
            260                 265                 270         


Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp Trp Thr Leu Ile 
        275                 280                 285             


Lys Lys Lys Thr Glu Gln Glu Ile Glu Gln Phe Asn Gln Thr Val Gly 
    290                 295                 300                 


Ser Tyr Ile Tyr Glu Ala Leu Leu Asp Asn Pro Thr Gln Lys Ile Arg 
305                 310                 315                 320 


Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Ser Glu Leu 
                325                 330                 335     


Lys Lys Ile Leu Glu Lys Gln Ile Glu Leu Gln Pro Glu Leu Phe Thr 
            340                 345                 350         


Glu Asp Leu Tyr Asn Ala Cys Ala Arg Glu Leu Tyr Arg Ser Asn Val 
        355                 360                 365             


Ala His Gln Gln Gln Leu Ser Ser Arg Asp Phe Val Tyr Leu Phe Leu 
    370                 375                 380                 


Asn Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser Gln Lys Ser Leu 
385                 390                 395                 400 


Ile Ser Asn Cys Ser Leu Glu Phe Arg Thr Ile Lys Asp Lys Asp Gly 
                405                 410                 415     


Asn Gly Gln Ile Ile Tyr Leu Lys Ala Ile Pro Lys Ser Asn Pro Tyr 
            420                 425                 430         


Tyr Gln Glu Phe Arg Val Trp Gln Trp Leu Tyr Asn Leu Lys Ile Phe 
        435                 440                 445             


Thr Lys Glu Asp Asp Lys Asp Val Thr Asn Gln Phe Ile Asn Lys Val 
    450                 455                 460                 


Glu Asp Leu Glu Ala Leu Phe Glu Phe Leu Met Asn Gln Lys Glu Val 
465                 470                 475                 480 


Asn His Thr Asp Ile Leu Thr Tyr Leu Ile Glu Pro Ile Val Lys Ser 
                485                 490                 495     


Lys Tyr Pro Asn Ala Lys Gly Lys Ala Phe Lys Asp Glu Leu Lys Lys 
            500                 505                 510         


Glu Leu Lys Lys Tyr Arg Trp Asn Tyr Val Tyr Asp Ala Asp Lys Asp 
        515                 520                 525             


Glu Ser Lys Ser Tyr Pro Met Asn Glu Thr Arg Tyr Glu Leu Arg Lys 
    530                 535                 540                 


Arg Leu Asp Lys Val Glu Asn Val Pro Ala Asp Phe Met Thr Arg Lys 
545                 550                 555                 560 


Ile Glu Gln His Leu Trp His Ile Ile Tyr Ser Val Thr Asp Lys Ile 
                565                 570                 575     


Glu Phe Glu Lys Ala Leu Lys Ser Phe Ala Asn Lys Tyr Asp Leu His 
            580                 585                 590         


Gln Glu Ser Phe Val Glu Asn Phe Lys Arg Phe Lys Pro Phe Asp Ser 
        595                 600                 605             


Asp Tyr Gly Thr Tyr Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu 
    610                 615                 620                 


Met Arg Val Gly Lys Tyr Trp Ser Trp Glu Gly Ile Asp Asn Asn Thr 
625                 630                 635                 640 


Lys Lys Arg Ile Gly Lys Ile Ile Thr Gly Glu Tyr Asp Glu Thr Ile 
                645                 650                 655     


Lys Asn Arg Val Arg Glu Lys Ala Ile Asp Leu Thr Glu Glu Asn His 
            660                 665                 670         


Phe Gln Gly Leu Gln Leu Trp Leu Ala Gln Tyr Ile Val Tyr Asp Arg 
        675                 680                 685             


His Ser Glu Ala Asp Ile Ala Gly Lys Trp Asn Ser Val Ala Asp Leu 
    690                 695                 700                 


Glu Gln Tyr Leu Ser Glu Phe Lys Gln His Ser Leu Arg Asn Pro Ile 
705                 710                 715                 720 


Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val Val Arg Asp Ile Trp 
                725                 730                 735     


Lys Gln Tyr Gly Lys Gly Ala Lys Asp Tyr Phe Asp Glu Ile His Ile 
            740                 745                 750         


Glu Leu Gly Arg Glu Met Lys Asn Thr Ala Glu Glu Arg Lys Arg Leu 
        755                 760                 765             


Ser Ser Ile Ile Ser Glu Asn Glu Thr Thr Asn Leu Arg Ile Arg Ala 
    770                 775                 780                 


Ile Leu Glu Glu Leu Met Tyr Asp Glu Asn Met Glu Asn Val Arg Pro 
785                 790                 795                 800 


Tyr Ser Pro Met Gln Gln Glu Ala Leu Lys Ile Tyr Glu Asp Gly Val 
                805                 810                 815     


Leu Ser Ser Ser Val Glu Ile Pro Glu Asp Ile Glu Lys Ile Ser Lys 
            820                 825                 830         


Lys Ser Glu Pro Thr Lys Ser Glu Ile Gln Arg Tyr Lys Leu Trp Leu 
        835                 840                 845             


Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Arg Pro Ile Pro Leu Ser 
    850                 855                 860                 


Lys Leu Phe Thr Pro Ala Tyr Gln Ile Glu His Ile Ile Pro Gln Ala 
865                 870                 875                 880 


Arg Phe Phe Asp Asp Gly Phe Ser Asn Lys Val Ile Cys Glu Ser Glu 
                885                 890                 895     


Val Asn Ala Leu Lys Asp Lys Met Leu Gly Leu Glu Phe Ile Gln Asn 
            900                 905                 910         


Tyr Tyr Gly Gln Lys Val Thr Thr Ser Phe Gly Glu Val Thr Ile Leu 
        915                 920                 925             


Thr Glu Asp Ala Tyr Lys Ala Phe Val Lys Glu His Tyr Asp Lys Asn 
    930                 935                 940                 


Arg Ser Lys Arg Asn Lys Leu Leu Leu Glu Glu Ile Pro Glu Lys Met 
945                 950                 955                 960 


Ile Asp Arg Gln Met Asn Asp Thr Arg Tyr Ile Ser Lys Tyr Ile Ser 
                965                 970                 975     


Lys Val Leu Ser Asn Leu Val Arg Gly Glu Lys Asp Asp Glu Gly Val 
            980                 985                 990         


Asn Ser Lys Asn Ile Ile Pro Gly  Asn Gly Lys Ile Ser  Gly Thr Leu 
        995                 1000                 1005             


Lys Gln  Asp Trp Gly Leu Asn  Asp Val Trp Asn Asp  Leu Ile Leu 
    1010                 1015                 1020             


Pro Arg  Phe Glu Arg Met Asn  Gln Leu Thr Asn Ser  Thr Asp Phe 
    1025                 1030                 1035             


Thr Ala  Trp Asn Glu Lys His  Gln Lys Phe Leu Pro  Thr Ile Pro 
    1040                 1045                 1050             


Leu Glu  Leu Ser Lys Gly Phe  Gln Lys Lys Arg Ile  Asp His Arg 
    1055                 1060                 1065             


His His  Ala Leu Asp Ala Leu  Ile Ile Ala Cys Ala  Thr Arg Asp 
    1070                 1075                 1080             


His Val  Asn Leu Leu Asn Asn  Lys His Ala Lys Ser  Arg Asn Glu 
    1085                 1090                 1095             


Arg Tyr  Asp Leu Gln His Lys  Leu Arg His Thr Thr  Pro Trp Gln 
    1100                 1105                 1110             


Asp Lys  Glu Gly Lys Val Arg  Thr Lys Phe Thr Glu  Phe Lys Lys 
    1115                 1120                 1125             


Pro Trp  Glu Thr Phe Thr Gln  Asp Thr Lys Lys Glu  Leu Glu Lys 
    1130                 1135                 1140             


Ile Val  Val Ser Phe Lys Gln  Asn Leu Arg Val Ile  Asn Lys Ala 
    1145                 1150                 1155             


Thr Asn  Tyr Tyr Glu Lys Ile  Val Asp Gly Lys Lys  Val Met Phe 
    1160                 1165                 1170             


Lys Gln  Glu Gly Val Asn Trp  Ala Ile Arg Lys Pro  Met His Lys 
    1175                 1180                 1185             


Glu Thr  Val Ser Gly Lys Ile  Asp Leu Pro Arg Val  Lys Val Ser 
    1190                 1195                 1200             


Lys Gly  Lys Ile Leu Thr Ala  Thr Arg Lys Ser Leu  Asp Thr Ser 
    1205                 1210                 1215             


Phe Asn  Ala Lys Thr Ile Leu  Ser Ile Thr Asp Thr  Gly Ile Gln 
    1220                 1225                 1230             


Lys Ile  Leu Leu Asn Tyr Leu  Lys Ala Lys Gly Asn  Pro Glu Leu 
    1235                 1240                 1245             


Ala Phe  Ser Pro Glu Gly Ile  Glu Glu Met Asn Gln  Asn Ile Ser 
    1250                 1255                 1260             


Leu Tyr  Asn Asp Gly Lys Pro  His Gln Pro Ile Leu  Lys Val Arg 
    1265                 1270                 1275             


Val Phe  Glu Gln Gly Ser Lys  Phe Pro Leu Gly Glu  Thr Gly Asn 
    1280                 1285                 1290             


Lys Thr  Thr Lys Tyr Val Glu  Ala Ala Lys Gly Thr  Asn Leu Phe 
    1295                 1300                 1305             


Phe Gly  Val Tyr Glu Asn Asn  Gln Gly Lys Arg Ser  Tyr Asn Thr 
    1310                 1315                 1320             


Ile Pro  Leu Asn Leu Val Ile  Glu Arg Gln Lys Gln  Gly Leu Asn 
    1325                 1330                 1335             


Ser Val  Pro Glu Thr Asn Glu  Lys Gly His Arg Leu  Leu Phe Ser 
    1340                 1345                 1350             


Leu Ser  Pro Asn Asp Leu Val  Tyr Val Pro Ile Glu  Gly Glu Val 
    1355                 1360                 1365             


Phe Asp  Lys Thr Asn Pro Asp  Val Asn Arg Ile Tyr  Lys Ile Val 
    1370                 1375                 1380             


Ser Phe  Ser Gly Ser Gln Met  Phe Cys Val Arg Gln  Asp Val Ala 
    1385                 1390                 1395             


Thr Ser  Ile Val Tyr Lys Leu  Glu Phe Ser Ser Leu  Asn Lys Met 
    1400                 1405                 1410             


Glu Arg  Thr Ile Asp Gly Ile  Met Ile Lys Glu Ile  Cys Ile Lys 
    1415                 1420                 1425             


Leu Lys  Val Asp Arg Leu Gly  Asn Ile Ser Lys Ala  
    1430                 1435                 1440 


<210>  1260
<211>  1440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1260

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Glu Gln Asp Phe Glu Asn Lys Gln Gly Leu Ile Leu Gly 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Met Asp Ala Gly Ile Ile Gly Lys Phe 
        35                  40                  45              


Ala Glu Gly Ser Ser Ile Ser Gln Thr Ala Glu Arg Thr Gly Tyr Arg 
    50                  55                  60                  


Ser Ile Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Asn Ile Leu Lys Phe Leu Pro Glu His Tyr Ala Ala 
                85                  90                  95      


Glu Ile Asp Phe Glu Lys Arg Phe Gly Gln Phe Leu Ala Glu Thr Glu 
            100                 105                 110         


Pro Lys Leu Ala Trp Lys Lys Asn Ala Glu Gly Lys Phe Glu Phe Leu 
        115                 120                 125             


Phe Gln Arg Ser Phe Asp Glu Met Val Ala Asp Phe Lys Ala Asn Gly 
    130                 135                 140                 


Gln Asp Ile Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys 
145                 150                 155                 160 


Lys Ala Leu Thr Gln Lys Ile Ser Pro Gln Glu Leu Ala Trp Ile Ile 
                165                 170                 175     


Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu 
            180                 185                 190         


Glu Glu Glu Asn Pro Asn Lys Lys Val Glu Phe Tyr Ser Leu Lys Ile 
        195                 200                 205             


Val Asp Val Val Ala Asp Glu Gln Pro Asn Lys Lys Gly Asp Thr Trp 
    210                 215                 220                 


Tyr Ser Leu His Leu Glu Asn Gly Trp Val Tyr Arg Arg Ser Ser Lys 
225                 230                 235                 240 


Ile Pro Leu Tyr Asp Trp Lys Asp Lys Val Arg Asp Phe Ile Val Thr 
                245                 250                 255     


Thr Asp Ile Asn Glu Asp Gly Ser Glu Lys Leu Asp Lys Asp Gly Glu 
            260                 265                 270         


Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp Trp Thr Leu Ile 
        275                 280                 285             


Lys Lys Lys Thr Glu Gln Glu Ile Glu Gln Phe Asn Gln Thr Val Gly 
    290                 295                 300                 


Ser Tyr Ile Tyr Glu Ala Leu Leu Asp Asn Pro Thr Gln Lys Ile Arg 
305                 310                 315                 320 


Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Ser Glu Leu 
                325                 330                 335     


Lys Lys Ile Leu Glu Lys Gln Ile Glu Leu Gln Pro Glu Leu Phe Thr 
            340                 345                 350         


Glu Asp Leu Tyr Asn Ala Cys Ala Arg Glu Leu Tyr Arg Arg Asn Val 
        355                 360                 365             


Ala His Gln Gln Gln Leu Ser Ser Arg Asp Phe Val Tyr Leu Phe Leu 
    370                 375                 380                 


Asn Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser Gln Lys Ser Leu 
385                 390                 395                 400 


Ile Ser Asn Cys Ser Leu Glu Phe Arg Thr Ile Lys Asp Lys Asp Gly 
                405                 410                 415     


Asn Gly Gln Ile Ile Tyr Leu Lys Ala Ile Pro Lys Ser Asn Pro Tyr 
            420                 425                 430         


Tyr Gln Glu Phe Arg Val Trp Gln Trp Leu Tyr Asn Leu Lys Ile Phe 
        435                 440                 445             


Thr Lys Glu Asp Asp Lys Asp Val Thr Asn Gln Phe Ile Asn Lys Val 
    450                 455                 460                 


Glu Asp Leu Glu Ala Leu Phe Glu Phe Leu Met Asn Gln Lys Glu Val 
465                 470                 475                 480 


Asn His Thr Asp Ile Leu Thr Tyr Leu Ile Glu Pro Ile Val Lys Ser 
                485                 490                 495     


Lys Tyr Pro Asn Ala Lys Gly Lys Ala Phe Lys Asp Glu Leu Lys Lys 
            500                 505                 510         


Glu Leu Lys Lys Tyr Arg Trp Asn Tyr Val Tyr Asp Ala Asp Lys Asp 
        515                 520                 525             


Glu Ser Lys Ser Tyr Pro Met Asn Glu Thr Arg Tyr Glu Leu Arg Lys 
    530                 535                 540                 


Arg Leu Asp Lys Val Glu Asn Val Pro Ala Asp Phe Met Thr Arg Lys 
545                 550                 555                 560 


Ile Glu Gln His Leu Trp His Ile Ile Tyr Ser Val Thr Asp Lys Ile 
                565                 570                 575     


Glu Phe Glu Lys Ala Leu Lys Ser Phe Ala Asn Lys Tyr Asp Leu His 
            580                 585                 590         


Gln Glu Ser Phe Val Glu Asn Phe Lys Arg Phe Lys Pro Phe Asp Ser 
        595                 600                 605             


Asp Tyr Gly Thr Tyr Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu 
    610                 615                 620                 


Met Arg Val Gly Lys Tyr Trp Ser Trp Asp Ile Ile Asp Asp Lys Thr 
625                 630                 635                 640 


Lys Thr Arg Ile Gly Lys Ile Ile Thr Gly Glu Tyr Asp Glu Thr Ile 
                645                 650                 655     


Lys Asn Arg Val Arg Glu Lys Ala Ile Asp Leu Thr Glu Glu Asn His 
            660                 665                 670         


Phe Gln Gly Leu Gln Leu Trp Leu Ala Gln Tyr Ile Val Tyr Asp Arg 
        675                 680                 685             


His Ser Glu Ala Asp Ile Ala Gly Lys Trp Asn Ser Val Ala Asp Leu 
    690                 695                 700                 


Glu Gln Tyr Leu Ser Glu Phe Lys Gln His Ser Leu Arg Asn Pro Ile 
705                 710                 715                 720 


Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val Val Arg Asp Ile Trp 
                725                 730                 735     


Lys Gln Tyr Gly Lys Gly Ala Lys Asp Tyr Phe Asp Glu Ile His Ile 
            740                 745                 750         


Glu Leu Gly Arg Glu Met Lys Asn Thr Ala Glu Glu Arg Lys Arg Leu 
        755                 760                 765             


Ser Ser Ile Ile Ser Glu Asn Glu Thr Thr Asn Leu Arg Ile Arg Ala 
    770                 775                 780                 


Ile Leu Glu Glu Leu Met Tyr Asp Glu Asn Met Glu Asn Val Arg Pro 
785                 790                 795                 800 


Tyr Ser Pro Met Gln Gln Glu Ala Leu Lys Ile Tyr Glu Asp Gly Val 
                805                 810                 815     


Leu Ser Ser Ser Val Glu Ile Pro Glu Asp Ile Glu Lys Ile Ser Lys 
            820                 825                 830         


Lys Ser Glu Pro Thr Lys Ser Glu Ile Gln Arg Tyr Lys Leu Trp Leu 
        835                 840                 845             


Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Arg Pro Ile Pro Leu Ser 
    850                 855                 860                 


Lys Leu Phe Thr Pro Ala Tyr Gln Ile Glu His Ile Ile Pro Gln Ala 
865                 870                 875                 880 


Arg Phe Phe Asp Asp Gly Phe Ser Asn Lys Val Ile Cys Glu Ser Glu 
                885                 890                 895     


Val Asn Ala Leu Lys Asp Lys Met Leu Gly Leu Glu Phe Ile Gln Asn 
            900                 905                 910         


Tyr Tyr Gly Gln Lys Val Thr Thr Ser Phe Gly Glu Val Thr Ile Leu 
        915                 920                 925             


Thr Glu Asp Ala Tyr Lys Ala Phe Val Lys Glu His Tyr Asp Lys Asn 
    930                 935                 940                 


Arg Ser Lys Arg Asn Lys Leu Leu Leu Glu Glu Ile Pro Glu Lys Met 
945                 950                 955                 960 


Ile Asp Arg Gln Met Asn Asp Thr Arg Tyr Ile Ser Lys Tyr Ile Ser 
                965                 970                 975     


Lys Val Leu Ser Asn Leu Val Arg Gly Glu Lys Asp Asp Glu Gly Val 
            980                 985                 990         


Asn Ser Lys Asn Ile Ile Pro Gly  Asn Gly Lys Ile Thr  Gly Ile Leu 
        995                 1000                 1005             


Lys Gln  Asp Trp Gly Leu Asn  Asp Val Trp Asn Asp  Leu Ile Leu 
    1010                 1015                 1020             


Pro Arg  Phe Glu Arg Met Asn  Gln Leu Thr Asn Ser  Thr Asp Phe 
    1025                 1030                 1035             


Thr Ala  Trp Asn Glu Lys His  Gln Lys Phe Leu Pro  Ser Val Pro 
    1040                 1045                 1050             


Leu Glu  Leu Ser Lys Gly Phe  Gln Lys Lys Arg Ile  Asp His Arg 
    1055                 1060                 1065             


His His  Ala Leu Asp Ala Leu  Ile Ile Ala Cys Ala  Thr Arg Asp 
    1070                 1075                 1080             


His Val  Asn Leu Leu Asn Asn  Lys His Ala Lys Ser  Arg Asn Glu 
    1085                 1090                 1095             


Arg Tyr  Asp Leu Gln His Lys  Leu Arg His Thr Thr  Pro Trp Gln 
    1100                 1105                 1110             


Asp Lys  Glu Gly Lys Val Arg  Thr Lys Phe Thr Glu  Phe Lys Lys 
    1115                 1120                 1125             


Pro Trp  Glu Thr Phe Thr Gln  Asp Thr Lys Lys Glu  Leu Glu Lys 
    1130                 1135                 1140             


Ile Val  Val Asn Phe Lys Gln  Asn Leu Arg Val Ile  Asn Lys Ala 
    1145                 1150                 1155             


Thr Asn  Tyr Tyr Glu Lys Ile  Val Asp Gly Lys Lys  Val Met Phe 
    1160                 1165                 1170             


Lys Gln  Glu Gly Val Asn Trp  Ala Ile Arg Lys Pro  Met His Lys 
    1175                 1180                 1185             


Glu Thr  Val Ser Gly Lys Ile  Asp Leu Pro Arg Val  Lys Val Ser 
    1190                 1195                 1200             


Lys Gly  Lys Ile Leu Thr Ala  Thr Arg Lys Ser Leu  Asp Thr Ser 
    1205                 1210                 1215             


Phe Asn  Ala Lys Thr Ile Leu  Ser Ile Thr Asp Thr  Gly Ile Gln 
    1220                 1225                 1230             


Lys Ile  Leu Leu Asn Tyr Leu  Lys Ala Lys Gly Asn  Pro Glu Leu 
    1235                 1240                 1245             


Ala Phe  Ser Pro Glu Gly Ile  Glu Glu Met Asn Gln  Asn Ile Ser 
    1250                 1255                 1260             


Leu Tyr  Asn Asp Gly Lys Pro  His Gln Pro Ile Leu  Lys Val Arg 
    1265                 1270                 1275             


Val Phe  Glu Gln Gly Ser Lys  Phe Pro Leu Gly Glu  Thr Gly Asn 
    1280                 1285                 1290             


Lys Thr  Thr Lys Tyr Val Glu  Ala Ala Lys Gly Thr  Asn Leu Phe 
    1295                 1300                 1305             


Phe Gly  Val Tyr Glu Asp Asn  Gln Gly Lys Arg Ser  Tyr Asp Thr 
    1310                 1315                 1320             


Ile Pro  Leu Asn Ile Val Ile  Glu Arg Gln Lys Gln  Gly Leu Asn 
    1325                 1330                 1335             


Ser Val  Pro Glu Thr Asn Glu  Lys Gly His Arg Leu  Leu Phe Ser 
    1340                 1345                 1350             


Leu Ser  Pro Asn Asp Leu Val  Tyr Val Pro Ile Glu  Gly Glu Val 
    1355                 1360                 1365             


Phe Asp  Glu Thr Asn Pro Asp  Val Asn Arg Ile Tyr  Lys Val Val 
    1370                 1375                 1380             


Ser Phe  Ser Gly Ser Gln Met  Phe Cys Val Arg Gln  Asp Val Ala 
    1385                 1390                 1395             


Thr Ser  Ile Val Tyr Lys Leu  Glu Phe Ser Ser Leu  Asn Lys Met 
    1400                 1405                 1410             


Glu Arg  Thr Ile Asp Gly Ile  Met Ile Lys Glu Ile  Cys Ile Lys 
    1415                 1420                 1425             


Leu Lys  Val Asp Arg Leu Gly  Asn Ile Ser Lys Ala  
    1430                 1435                 1440 


<210>  1261
<211>  1443
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1261

Met Ile Lys Leu Leu Tyr Leu Glu Lys Leu Lys Gln Tyr Thr Met Lys 
1               5                   10                  15      


Lys Ile Leu Gly Leu Asp Leu Gly Thr Ser Ser Ile Gly Trp Ala Leu 
            20                  25                  30          


Val Asn Glu Ala Glu Asn Ser Asp Glu Val Ser Ser Ile Ile Lys Leu 
        35                  40                  45              


Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Met Gln Asn Phe Glu 
    50                  55                  60                  


Lys Gly Lys Ser Ile Thr Thr Asn Ala Asp Arg Thr Leu Lys Arg Ser 
65                  70                  75                  80  


Met Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Asp Ala Leu Ile 
                85                  90                  95      


Glu Leu Leu Lys Lys Val Gly Phe Ile Thr Asp Lys Thr Ile Leu Ser 
            100                 105                 110         


Glu Gln Gly Asn Arg Thr Thr Phe Glu Thr Tyr Arg Leu Arg Ala Lys 
        115                 120                 125             


Ala Ala Glu Glu Glu Ile Ser Leu Lys Glu Leu Ala Arg Val Leu Leu 
    130                 135                 140                 


Met Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala Lys Gly 
145                 150                 155                 160 


Glu Glu Glu Gly Ala Leu Ile Asp Gly Met Glu Ile Ala Lys Lys Leu 
                165                 170                 175     


Tyr Asp Glu Asp Leu Thr Pro Gly Gln Leu Cys Leu Gln Leu Leu Glu 
            180                 185                 190         


Ser Gly Lys Lys Phe Phe Pro Asp Phe Tyr Arg Ser Asp Leu Gln Glu 
        195                 200                 205             


Glu Phe Asn Lys Ile Trp Asp Phe Gln Lys Gln Phe Asn Leu Phe Ser 
    210                 215                 220                 


Phe Cys Asp Val Ala Lys Glu Glu Val Lys Gly Lys Asn Lys Ser Gln 
225                 230                 235                 240 


Thr Trp Ala Ile Leu Ala Lys Tyr Phe Val Glu Thr Lys Lys Val Asn 
                245                 250                 255     


Val Trp Asn Glu His Glu Ala Gln Thr Glu Asn Arg Glu Glu Val Tyr 
            260                 265                 270         


Lys Leu Val Gly Leu Lys Arg Thr Val Lys Gly Glu Glu Leu Lys Lys 
        275                 280                 285             


Glu Asn Tyr Arg Trp Arg Val Gln Ala Leu Thr Glu Gln Leu Asp Leu 
    290                 295                 300                 


Glu Lys Ile Ala Val Val Leu Gln Glu Ile Asn Gly Gln Ile Ser Ala 
305                 310                 315                 320 


Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Val Leu Tyr 
                325                 330                 335     


Phe Asn Arg Gln Thr Val Gly Gln Tyr Gln Met Ala Glu Leu Asn Lys 
            340                 345                 350         


Asn Pro Asn Met Ser Leu Arg Asn Met Val Phe Tyr Arg Gln Asp Tyr 
        355                 360                 365             


Leu Asp Glu Phe Asn Val Ile Trp Glu Lys Gln Ala Glu Phe His Lys 
    370                 375                 380                 


Glu Leu Thr Pro Glu Leu Lys Lys Glu Ile Arg Asp Ile Ile Ile Phe 
385                 390                 395                 400 


Tyr Gln Arg Arg Leu Lys Ser Gln Lys Gly Leu Ile Ser Phe Cys Glu 
                405                 410                 415     


Phe Glu Asn Arg Glu Val Val Val Glu Lys Glu Gly Lys Lys Gln Thr 
            420                 425                 430         


Lys Ile Ile Gly Cys Lys Val Ile Pro Arg Ser His Pro Leu Phe Gln 
        435                 440                 445             


Glu Phe Lys Ile Trp Gln Thr Leu Asn Asn Ile Glu Val Phe Val Gly 
    450                 455                 460                 


Glu Lys Lys Gly Lys Arg Lys Lys Ser Asn Val Ser Ser Asp Leu Phe 
465                 470                 475                 480 


Gly Asp Thr Glu Asp Ala Leu Ile Thr Glu Gly Arg Arg Phe Leu Tyr 
                485                 490                 495     


Gln Glu Glu Lys Lys Ile Leu Ala Lys Glu Leu Ser Ile Lys Lys Gly 
            500                 505                 510         


Leu Thr Lys Ala Glu Ile Leu Arg Leu Leu Phe Glu Asn Pro Gln Glu 
        515                 520                 525             


Leu Asp Ile Asn Phe Lys Gln Ile Asp Gly Asn Gln Thr Gly Phe Ala 
    530                 535                 540                 


Leu Phe Ser Ala Tyr Ser Asn Met Ile Glu Lys Tyr Gly Tyr Glu Pro 
545                 550                 555                 560 


Leu Asp Phe Lys Lys Pro Ala Glu Asp Leu Ile Ser Gln Leu Lys Thr 
                565                 570                 575     


Val Phe Thr Asp Leu Gly Trp Asn Thr Asp Leu Leu Phe Ile Asp Leu 
            580                 585                 590         


Asp Lys Glu Asn Lys Glu Trp Glu Lys Gln Pro Tyr Phe Arg Leu Trp 
        595                 600                 605             


His Leu Leu Tyr Ser Phe Glu Gly Asp Asn Thr Pro Thr Gly Asn Gly 
    610                 615                 620                 


Lys Leu Ile Glu Lys Ile Met Gln Leu Cys Gly Ile Glu Lys Glu Tyr 
625                 630                 635                 640 


Ala Ile Glu Leu Ala Asn Val Ser Leu Gln Asp Asp Tyr Gly Ser Leu 
                645                 650                 655     


Ser Ala Lys Ala Ile Lys Lys Ile Leu Leu Tyr Leu Lys Glu Gly Asn 
            660                 665                 670         


Gln Tyr Asp Met Ala Cys Glu Tyr Ala Gly Tyr Arg His Ser Lys Ser 
        675                 680                 685             


Ser Leu Thr Lys Glu Glu Ile Glu Asn Lys Val Leu Lys Asp Lys Leu 
    690                 695                 700                 


Glu Ile Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile 
705                 710                 715                 720 


Leu Asn Gln Met Val Asn Val Ile Asn Thr Val Met Glu Val Tyr Gly 
                725                 730                 735     


Arg Pro Asp Glu Ile Arg Val Glu Leu Ala Arg Glu Leu Lys Lys Ser 
            740                 745                 750         


Ala Lys Glu Arg Glu Glu Leu Thr Lys Ala Val Ala Lys Asn Thr Arg 
        755                 760                 765             


Glu His Glu Glu Ile Arg Lys Val Leu Gln Ser Glu Phe Gly Met Ile 
    770                 775                 780                 


Asn Val Ser Arg Asn Asp Ile Ile Arg Tyr Lys Leu Tyr Glu Glu Leu 
785                 790                 795                 800 


Lys Asp Asn Gly Tyr Lys Thr Leu Tyr Ser Asn Gln Tyr Ile Pro Arg 
                805                 810                 815     


Glu Lys Leu Phe Ser Lys Glu Ile Asp Ile Glu His Ile Ile Pro Gln 
            820                 825                 830         


Ser Arg Leu Phe Asp Asp Ser Leu Ser Asn Lys Thr Leu Glu Tyr Lys 
        835                 840                 845             


Ser Ile Asn Ile Glu Lys Gly Asn Lys Thr Ala Tyr Asp Phe Val Lys 
    850                 855                 860                 


Glu Lys Tyr Gly Glu Glu Gly Leu Gln Gln Tyr Leu Asn Arg Cys Glu 
865                 870                 875                 880 


Asn Leu Cys Lys Asp Lys Lys Ala Lys Leu Arg Lys Leu Lys Met Glu 
                885                 890                 895     


Leu Asn Glu Ile Pro Asp Gly Phe Ile Asp Arg Asp Leu Arg Asn Thr 
            900                 905                 910         


Gln Tyr Ile Ala Lys Lys Ala Leu Ser Met Leu Thr Glu Ile Cys Arg 
        915                 920                 925             


Arg Val Val Ala Thr Thr Gly Ser Ile Thr Asp Lys Leu Arg Glu Asp 
    930                 935                 940                 


Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn Leu Pro Lys Tyr Glu 
945                 950                 955                 960 


Ala Leu Gly Leu Val Glu Val Tyr Glu Asp Lys Asp Gly Arg Lys Ile 
                965                 970                 975     


Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp His Arg His His Ala 
            980                 985                 990         


Met Asp Ala Leu Thr Val Ala Phe  Thr Lys Asp Val Phe  Ile Gln Tyr 
        995                 1000                 1005             


Phe Asn  Asn Lys Asn Ala Ala  Trp Glu Ser Asp Ser  Lys Glu His 
    1010                 1015                 1020             


Thr Asn  Ile Val Gly Ile Lys  Thr Arg Tyr Phe Glu  Ser Gly Lys 
    1025                 1030                 1035             


Ala Val  Pro Pro Ile Pro Leu  Gly Gln Phe Arg Val  Glu Ala Lys 
    1040                 1045                 1050             


Cys His  Leu Glu Asn Leu Leu  Val Ser Ile Lys Ala  Lys Asn Lys 
    1055                 1060                 1065             


Val Val  Thr Thr Asn Val Asn  Cys Ile Lys Lys Lys  Gly Gly Thr 
    1070                 1075                 1080             


Tyr Lys  Asn Thr Gln Gln Thr  Pro Arg Gly Gln Leu  His Leu Glu 
    1085                 1090                 1095             


Thr Ile  Tyr Gly Ser His Asn  Gln Tyr Val Thr Lys  Ile Glu Lys 
    1100                 1105                 1110             


Val Asn  Ala Ser Phe Asp Ala  Val Lys Ile Ala Thr  Val Ser Lys 
    1115                 1120                 1125             


Gln Asp  Tyr Arg Asn Ala Leu  Leu Lys Arg Leu Glu  Val Phe Gly 
    1130                 1135                 1140             


Asn Asp  Pro Lys Lys Ala Phe  Thr Gly Lys Asn Ser  Leu Glu Lys 
    1145                 1150                 1155             


Asn Pro  Ile Tyr Leu Asp Arg  Ala Gln Thr Ile Gln  Val Pro Asp 
    1160                 1165                 1170             


Lys Val  Gln Thr Val Glu Leu  Glu Thr Ala Tyr Thr  Ile Arg Lys 
    1175                 1180                 1185             


Pro Val  Asp Pro Thr Leu Asn  Val Asp Lys Val Val  Asp Lys Lys 
    1190                 1195                 1200             


Ile Arg  Leu Ile Leu Glu Gln  Arg Leu Lys Glu Tyr  Gly Gly Asp 
    1205                 1210                 1215             


Ala Lys  Lys Ala Phe Val Asn  Leu Asp Glu Asn Pro  Ile Trp Leu 
    1220                 1225                 1230             


Asn Glu  Glu Lys Gly Ile Ser  Ile Lys Arg Val Thr  Ile Arg Gly 
    1235                 1240                 1245             


Ile Ser  Lys Ala Gln Ala Leu  His Asp Lys Lys Asp  Lys Asp Gly 
    1250                 1255                 1260             


Asn Phe  Ile Leu Asp Glu Thr  Gly Lys Arg Ile Pro  Val Asp Phe 
    1265                 1270                 1275             


Val Asn  Thr Gly Asn Asn His  His Val Ala Ile Tyr  Arg Lys Pro 
    1280                 1285                 1290             


Val Leu  Asp Lys Lys Gly Gln  Val Ile Phe Asp Asp  Glu Gly Asn 
    1295                 1300                 1305             


Pro Met  Tyr Glu Leu Asp Glu  Asp Val Val Pro Phe  Phe Glu Ala 
    1310                 1315                 1320             


Val Thr  Arg Ala Asn Leu Gly  Ile Pro Ile Ile Asp  Lys Asp Tyr 
    1325                 1330                 1335             


Lys Lys  Ser Glu Gly Trp Gln  Phe Leu Phe Ser Met  Lys Gln Asn 
    1340                 1345                 1350             


Glu Tyr  Phe Val Phe Pro Asn  Glu Lys Thr Gly Phe  Asn Pro Lys 
    1355                 1360                 1365             


Glu Val  Asp Leu Leu Asp Pro  Asp Asn Tyr Ala Met  Ile Ser Pro 
    1370                 1375                 1380             


Asn Leu  Phe Arg Val Gln Lys  Ile Gly Leu Asn Asp  Tyr Thr Phe 
    1385                 1390                 1395             


Arg His  His Leu Glu Thr Thr  Ile Gln Asn Asp Leu  Lys Phe Ile 
    1400                 1405                 1410             


Thr Trp  Ile Arg Cys Gly Lys  Ser Gly Ile Lys Asp  Ile Val Lys 
    1415                 1420                 1425             


Val Arg  Ile Asn His Ile Gly  Gln Ile Val Ser Val  Gly Glu Tyr 
    1430                 1435                 1440             


<210>  1262
<211>  1443
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1262

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Ala Ser Ile Gly Ser 
1               5                   10                  15      


Ala Tyr Val Leu Glu Ala Glu Lys Ser Ala Asp Gln Thr Lys Ile Val 
            20                  25                  30          


Tyr Leu Gly Thr Arg Ile Ile Leu Ser Ser Ala Asp Ala Glu Asn Phe 
        35                  40                  45              


Ser Lys Gly Leu Ser Cys Ser Pro Thr Thr Asp Arg Thr Phe Lys Arg 
    50                  55                  60                  


Ser Met Arg Arg Arg Leu Gln Arg Tyr Lys Gln Arg Arg Ser Ser Leu 
65                  70                  75                  80  


Ile Gln Gln Phe Lys Lys Ile Gly Ile Ile Thr Asn Thr Glu Gln Ile 
                85                  90                  95      


Ile Arg Glu Pro Ile Ser Glu Thr His Glu Leu Leu Arg Ile Arg Ser 
            100                 105                 110         


Lys Ala Val Ser Glu His Val Ser Leu Glu Glu Phe Ala Arg Ile Leu 
        115                 120                 125             


Leu Thr Ile Asn Lys Arg Arg Gly Tyr Lys Ser Asn Arg Lys Thr Ser 
    130                 135                 140                 


Lys Ser Thr Glu Glu Asp Gly Lys Leu Val Asp Ala Met Ala Val Thr 
145                 150                 155                 160 


Lys Glu Leu Ile Lys Leu Lys Lys Thr Pro Gly Gln Tyr Met Phe Glu 
                165                 170                 175     


Arg Phe Lys Arg Gly Lys Tyr Ala Glu Ile Asp Phe Tyr Pro Phe Asp 
            180                 185                 190         


Leu Ile Asn Glu Leu Lys Gln Ile Trp Glu Val Gln Gly Gln Lys Tyr 
        195                 200                 205             


Pro Asp Val Leu Thr Glu Gly Leu Leu Ser Ser Leu Leu Lys Gln Lys 
    210                 215                 220                 


Thr Gly Lys Ala Lys Glu Leu Ile Tyr Ser Lys Ser Asp Leu Asn Pro 
225                 230                 235                 240 


Ala Glu Val Pro Ile Asp Tyr Gln Lys Gly Lys Lys Leu Arg Lys Thr 
                245                 250                 255     


Ile Leu Asn Lys Tyr Phe Ala Tyr Glu Ser Arg Ser Leu Ala Val Thr 
            260                 265                 270         


Asn Ile Ile Pro Val Glu Gln Ala Ile Thr Ala Ile Val Ala Ile Cys 
        275                 280                 285             


Lys Gln Ile Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Gly Asp Arg 
    290                 295                 300                 


Ser Arg Val Leu Tyr Ala Asn Asn Gln Thr Ile Gly Gln Tyr Leu Tyr 
305                 310                 315                 320 


Glu Arg Ile Lys Ala Asn Pro His Asp Arg Ile Arg Gly Glu Val Phe 
                325                 330                 335     


Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Asp Arg Ile Trp Glu Cys Gln 
            340                 345                 350         


Gln Asn Tyr Tyr Pro Ala Ile Leu Thr Asn Asp Arg Lys Gln Tyr Ile 
        355                 360                 365             


Arg Asn Arg Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser 
    370                 375                 380                 


Glu Leu Ala Phe Cys Glu Phe Glu Ser Arg Glu Val Glu His Asn Gly 
385                 390                 395                 400 


Lys Lys Tyr Thr Glu Gly Leu His Val Ala Pro Lys Ser Ser Pro Val 
                405                 410                 415     


Phe Gln His Phe Arg Leu Trp Gln Arg Leu Gly Gln Ile Glu Val Ser 
            420                 425                 430         


Pro Leu Arg Lys Trp Asp Lys Gln Ser Asn Glu Leu Phe Glu Gln Gln 
        435                 440                 445             


Asn Asn Ala Asp Arg Glu Gly Ile Leu Asn Thr Glu Ala Lys Gln Lys 
    450                 455                 460                 


Leu Phe Ser Tyr Leu Asn Leu Leu Asp His Leu Thr Asp Asn Glu Ile 
465                 470                 475                 480 


Leu Asn Ile Leu Gly Tyr Ser Pro Asp Lys Trp Lys Ile Asn Phe Arg 
                485                 490                 495     


Glu Ile Ile Gly Asn Arg Thr Asn Asn Leu Met Met Lys Ala Phe Phe 
            500                 505                 510         


Ser Met Lys Glu Met Thr Asp Gly Glu Gln Ile Asp Trp Glu Lys Asn 
        515                 520                 525             


Leu Thr Lys Ile Thr Asn Gly Gln Ser Leu Ile Ser Pro Glu Gln Ile 
    530                 535                 540                 


Val Asp Leu Ile Arg Gln Phe Phe Glu Arg Glu Gly Ile Thr Thr Ser 
545                 550                 555                 560 


Leu Leu Tyr Phe Asp Pro Ala Thr Asp Asn Phe Arg Glu Gln Ser His 
                565                 570                 575     


Tyr Ser Leu Trp His Leu Leu Tyr Ser Tyr Glu Asp Asp Asn Ser Pro 
            580                 585                 590         


Thr Gly Thr Thr Ser Leu Cys Lys Thr Leu Ala Lys Arg Tyr Gly Ile 
        595                 600                 605             


Pro His Glu Leu Cys Pro Leu Leu Ala Ser Leu Thr Phe Glu Arg Gln 
    610                 615                 620                 


Tyr Gly Ser Leu Ser Thr Lys Ala Met Arg Lys Leu Leu Pro Phe Met 
625                 630                 635                 640 


Gln Glu Lys Arg Tyr Asp Glu Ala Cys Glu Leu Ala Gly Tyr Arg His 
                645                 650                 655     


Ser Ala Trp Ile Thr Lys Glu Glu Asn Ala Lys Arg Thr Leu Lys Asn 
            660                 665                 670         


Lys Leu Pro Leu Leu Arg Lys Gly Ser Leu Arg Asn Pro Val Val Glu 
        675                 680                 685             


Lys Ile Leu Asn Gln Met Thr His Val Ile Asn Thr His Ile Ala Glu 
    690                 695                 700                 


Tyr Gly Asn Pro Asp Glu Ile Arg Ile Glu Met Ala Arg Asp Met Gln 
705                 710                 715                 720 


Ala Asn Ser Lys Gln Arg Lys Ala Met Thr Ala Ala Ile Thr Glu Ala 
                725                 730                 735     


Thr Lys Arg Asn Thr Gln Phe Ile Glu Glu Ile Gln Lys Glu Phe Gly 
            740                 745                 750         


Phe Asn Pro Ser Lys Asn Asp Leu Ile Lys Tyr Lys Leu Trp Lys Glu 
        755                 760                 765             


Leu Ala Pro Leu Gly Asn Lys Thr Leu Tyr Ser Asn Ile Tyr Ile Pro 
    770                 775                 780                 


Lys Glu Glu Leu Phe Thr Ala Lys Phe Glu Ile Glu His Ile Ile Pro 
785                 790                 795                 800 


Arg Ala Leu Phe Tyr Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu Leu 
                805                 810                 815     


Asn Ser Val Asn Lys Leu Lys Gly Asn Lys Thr Ala Leu Asp Tyr Leu 
            820                 825                 830         


Asp Thr Leu Gly Gln Glu Lys Ala Asn Asp Tyr Ile Leu Arg Val Glu 
        835                 840                 845             


Glu Leu Lys Lys Ile Gly Ala Ile Thr Gln Ala Lys Tyr Lys Lys Leu 
    850                 855                 860                 


Leu Ile Arg Glu Lys Asp Leu Gly Asp Asp Phe Thr Asn Arg Asp Leu 
865                 870                 875                 880 


Thr Asn Thr Arg Tyr Ile Ile Arg His Ala Ala Asp Arg Asp Leu Thr 
                885                 890                 895     


Asn Thr Arg Tyr Ile Ile Arg His Ala Ala Asp Met Leu Leu Gln Val 
            900                 905                 910         


Thr Pro Ser Ile Val His Thr Asn Gly Ser Ile Thr Ala Ile Leu Arg 
        915                 920                 925             


Arg Asp Trp Gly Leu Leu Asp Ile Leu Lys Glu Leu Asn Leu Pro Thr 
    930                 935                 940                 


Tyr Glu Ser Ile Asp Arg Lys Ile Ser Asp Pro Glu Glu Lys Leu Thr 
945                 950                 955                 960 


Gln Ser Val Asp Arg Lys Asp Gly Gln Ala Ile Lys Met Ile Lys Asp 
                965                 970                 975     


Trp Ser Lys Arg Asp Asp His Arg His His Ala Met Asp Ala Leu Ile 
            980                 985                 990         


Thr Ala Leu Thr Thr Arg Lys His  Val Gln Tyr Leu Asn  Gly Asn Ser 
        995                 1000                 1005             


Gly Ala  Glu Glu Thr Asp Lys  Ala Gln Lys Leu Glu  Gln Leu Lys 
    1010                 1015                 1020             


Ala Leu  Arg Lys Gln Leu Ile  Gly Ser Asp Gly His  Phe Ile Pro 
    1025                 1030                 1035             


Pro Met  His Asp Ile Arg Val  Gln Ala Lys Glu Ala  Leu Ser Arg 
    1040                 1045                 1050             


Val Ile  Ile Ser Thr Lys Val  Lys Gly Lys Val Thr  Thr Pro Asn 
    1055                 1060                 1065             


Thr Asn  Ile Ile Cys Ser Arg  Asp Lys Thr Thr Gly  Arg Lys Ile 
    1070                 1075                 1080             


Tyr Val  Arg Gln Thr Thr Leu  Thr Pro Arg Gly Ala  Leu His Leu 
    1085                 1090                 1095             


Asp Thr  Lys Tyr Gly Ile Asn  Arg Tyr Tyr Gln Glu  Arg Glu Val 
    1100                 1105                 1110             


Ser Ile  Gly Ser Lys Leu Thr  Gln Glu Glu Ile Arg  Leu Val Ser 
    1115                 1120                 1125             


Ser Pro  His Ile Gln Ser Ile  Leu Leu Glu Arg Leu  Arg Ala Phe 
    1130                 1135                 1140             


Asp Asn  Asn Pro Lys Ser Ala  Phe Thr Gly Arg Asn  Ser Val Asp 
    1145                 1150                 1155             


Lys Lys  Pro Leu Glu Asp Lys  Asn Lys Lys Leu Val  Tyr Thr Asp 
    1160                 1165                 1170             


Lys Lys  Val Lys Gln Thr Ile  Leu Ala Asn Arg Phe  Val Lys Arg 
    1175                 1180                 1185             


Asp Arg  Val Asn Ser Lys Leu  Asp Leu Asp Asn Val  Ile Asp Thr 
    1190                 1195                 1200             


Gly Ile  Lys Arg Ile Leu Glu  Arg Arg Leu Lys Gln  Tyr Gly Gly 
    1205                 1210                 1215             


Lys Tyr  Glu Thr Ala Phe Ser  Asp Leu Glu Asn Asn  Pro Ile Trp 
    1220                 1225                 1230             


Leu Asn  Glu Glu Lys Gly Ile  Ala Ile Lys Ser Val  Arg Ile Arg 
    1235                 1240                 1245             


Gly Lys  Asn Asn Thr Ile Thr  Ile Arg Gln Thr Asn  Thr Cys Arg 
    1250                 1255                 1260             


Ile Lys  Tyr Val Ala Asn Asp  Asn Asn His His Leu  Ala Val Tyr 
    1265                 1270                 1275             


Ala Leu  Pro Asp Gly Ser Ile  Lys Asp Asp Ile Val  Ser Phe Phe 
    1280                 1285                 1290             


Asp Ala  Val Glu Arg Ser Arg  Lys Arg Leu Pro Ile  Ile Asp Lys 
    1295                 1300                 1305             


His Lys  Asn Gln Ser Leu Gly  Trp Lys Tyr Leu Phe  Ser Ile Lys 
    1310                 1315                 1320             


Gln Asn  Glu Tyr Phe Leu Leu  Pro Gln Gln Val Glu  Lys Ile Asp 
    1325                 1330                 1335             


Ile Asp  Thr Gly Glu Ile Ser  Met Gln Thr Thr Phe  Ser Pro Lys 
    1340                 1345                 1350             


Asp Leu  Ser His Glu Tyr Ile  Met Asn Pro Asp Asn  Tyr Ala Glu 
    1355                 1360                 1365             


Leu Ala  Ser His Ile Phe Arg  Val Gln Ser Ile Ser  Lys Thr Pro 
    1370                 1375                 1380             


Gln Gly  Asn Arg Asp Val Arg  Ile Tyr Leu Phe Lys  Gln Gln Tyr 
    1385                 1390                 1395             


Ile Ser  Gly Ser Gln Lys Asp  Ser Leu His Leu Lys  Gly Ile Thr 
    1400                 1405                 1410             


His Arg  Gln Ile Ser Ser Ala  Lys Asp Leu Ala Glu  Leu Val Lys 
    1415                 1420                 1425             


Ile Arg  Val Asn His Leu Gly  Arg Ile Val Ser Val  Gly Glu Lys 
    1430                 1435                 1440             


<210>  1263
<211>  1446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1263

Met Val Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Leu Ile Lys Gln Asp Phe Glu Asn Lys Gln Gly Asp Ile Leu 
            20                  25                  30          


Gly Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly Asp 
        35                  40                  45              


Phe Gly Lys Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg Thr Lys Tyr 
    50                  55                  60                  


Arg Ser Val Arg Arg Leu Arg Glu Arg Phe Leu Leu Arg Arg Glu Arg 
65                  70                  75                  80  


Leu His Arg Val Leu Asn Val Leu Asp Phe Leu Pro Glu His Tyr Ala 
                85                  90                  95      


Ser Gln Ile Asp Phe Asn Lys Arg Phe Gly Lys Phe Lys Glu Glu Thr 
            100                 105                 110         


Glu Pro Lys Leu Ala Tyr Ser Asn Asp Gly Phe Ile Phe Lys Lys Ser 
        115                 120                 125             


Phe Glu Glu Met Ile Ser Asp Phe Lys Thr His Gln Pro Glu Leu Leu 
    130                 135                 140                 


Glu Asn Asn Lys Lys Val Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg 
145                 150                 155                 160 


Asn Lys Ala Leu Ser His Lys Ile Glu Lys Glu Glu Leu Ala Trp Ile 
                165                 170                 175     


Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu 
            180                 185                 190         


Glu Glu Glu Glu Asn Pro Asn Lys Leu Ile Glu Phe Tyr Ser Leu Lys 
        195                 200                 205             


Ile Val Asp Val Leu Ala Asp Glu Pro Gln Lys Gly Lys Ser Asp Ile 
    210                 215                 220                 


Trp Tyr Ser Leu Ile Leu Glu Asn Gly Trp Ile Tyr Arg Arg Ser Ser 
225                 230                 235                 240 


Lys Ile Pro Leu Leu Asp Trp Lys Gly Lys Thr Arg Asp Phe Ile Val 
                245                 250                 255     


Thr Thr Asp Leu Asn Asp Asp Gly Ser Val Lys Thr Asp Lys Glu Gly 
            260                 265                 270         


Asn Glu Lys Arg Ser Phe Arg Ala Pro Gly Glu Asn Asp Trp Thr Leu 
        275                 280                 285             


Val Lys Lys Lys Thr Glu Gln Glu Ile Asp Asn Ser Asp Lys Thr Val 
    290                 295                 300                 


Gly Ala Tyr Ile Tyr Glu Ala Leu Leu Gln Asn Pro Lys Gln Lys Ile 
305                 310                 315                 320 


Lys Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Ala Glu 
                325                 330                 335     


Leu Lys Gln Ile Leu Glu Lys Gln Met Glu Phe His Pro Glu Leu Arg 
            340                 345                 350         


Gln Asp Asp Leu Tyr Ser Asp Cys Val Arg Glu Leu Tyr Lys Asn Asn 
        355                 360                 365             


Glu Ala His Gln Leu Thr Leu Ser Lys Lys Asp Phe Val His Leu Phe 
    370                 375                 380                 


Met Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser Gln Lys Ser 
385                 390                 395                 400 


Ser Ile Ser Asn Cys Thr Leu Glu Phe Arg Lys Tyr Lys Asp Glu Asn 
                405                 410                 415     


Gly Thr Glu His Ile Gln Tyr Leu Lys Ala Leu Pro Lys Ser Asn Pro 
            420                 425                 430         


Tyr Tyr Gln Glu Phe Arg Ile Trp Gln Trp Met Tyr Asn Leu Asn Ile 
        435                 440                 445             


Tyr Lys Lys Glu Asp Asp Thr Asn Val Thr Lys Glu Phe Leu Asn Ser 
    450                 455                 460                 


Lys Ala Asp Phe Glu Ser Leu Phe Glu Phe Leu Asn Asn Lys Lys Glu 
465                 470                 475                 480 


Val Asp Gln Lys Ala Leu Leu Lys His Phe Lys Ile Asn Asp Lys Thr 
                485                 490                 495     


His Arg Trp Lys Tyr Val Glu Asp Lys Lys Tyr Pro Cys Asn Glu Thr 
            500                 505                 510         


Lys Thr Met Ile Ser Glu Arg Leu Lys Lys Val Glu Asn Ile Ser Asp 
        515                 520                 525             


Asp Phe Leu Thr Arg Glu Met Glu Gln Lys Ile Trp His Ile Ile Tyr 
    530                 535                 540                 


Ser Val Asn Asp Lys Val Lys Tyr Glu Lys Ala Leu Lys Ser Phe Ala 
545                 550                 555                 560 


Glu Lys Asn Asn Leu Asp Glu Ser Ser Phe Phe Glu Ala Phe Lys Lys 
                565                 570                 575     


Phe Pro Pro Phe Lys Ser Glu Tyr Gly Ala Phe Ser Glu Lys Ala Ile 
            580                 585                 590         


Lys Lys Leu Leu Pro Leu Met Arg Leu Gly Lys Tyr Trp Asp Asp Asp 
        595                 600                 605             


Glu Ile Leu Lys Tyr Ser Asp Ile Tyr Phe Lys Asn Ile Glu Tyr Leu 
    610                 615                 620                 


Leu Gly Val Leu Lys Gln Lys Asp Lys Asn Ile Ser Asp Asp Asn Arg 
625                 630                 635                 640 


Gln Asn Trp Asn Lys Ser Ile Asn Asn Lys Leu Arg Glu Glu Leu Glu 
                645                 650                 655     


Ile Phe Gln Asn Ala Glu Ile Glu Ser Phe Gln Gly Leu Arg Leu His 
            660                 665                 670         


Ile Ala Gln Tyr Ile Leu Tyr Gly Arg His Ser Glu Ala Ala Met Leu 
        675                 680                 685             


Gly Lys Trp Asn Ser Ala Asp Asp Leu Glu Glu Phe Leu Lys Glu Phe 
    690                 695                 700                 


Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu 
705                 710                 715                 720 


Thr Leu Arg Val Val Lys Asp Ile Trp Val Lys Tyr Gly Asn Gly Ala 
                725                 730                 735     


Lys Asp Phe Phe Asn Glu Ile His Ile Glu Leu Gly Arg Glu Met Lys 
            740                 745                 750         


Leu Pro Ala Glu Asp Arg Lys Lys Ile Thr Asn Gln Ile Ser Glu Asn 
        755                 760                 765             


Glu Asn Thr Asn Leu Arg Ile Lys Ala Leu Leu Ala Glu Met Met Val 
    770                 775                 780                 


Asp Ser Ser Val Glu Asn Val Arg Pro Phe Ser Pro Met Gln Gln Glu 
785                 790                 795                 800 


Ile Leu Lys Ile Tyr Glu Asp Gly Val Leu Asn Ser Asp Ile Glu Ile 
                805                 810                 815     


Glu Asp Asp Ile Leu Lys Ile Ser Lys Thr Ala Gln Pro Ser Pro Ser 
            820                 825                 830         


Asp Leu Lys Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Lys Ser Pro 
        835                 840                 845             


Tyr Thr Gly Gln Ile Ile Pro Leu Asn Lys Leu Phe Thr Pro Glu Tyr 
    850                 855                 860                 


Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe 
865                 870                 875                 880 


Ser Asn Lys Val Ile Cys Glu Ala Ala Val Asn Lys Leu Lys Asp Asn 
                885                 890                 895     


Phe Ile Gly Leu Gly Phe Ile Lys Gln Phe Gly Gly Thr Ile Val Glu 
            900                 905                 910         


Cys Gly Asn Gly Lys Ser Val Thr Val Leu Lys Glu Asp Gln Tyr Glu 
        915                 920                 925             


Asp Phe Val Lys Lys His Tyr Ala Asn Asn Arg Ser Lys Arg Asn Lys 
    930                 935                 940                 


Leu Leu Leu Glu Asp Ile Pro Glu Lys Met Ile Glu Arg Gln Met Asn 
945                 950                 955                 960 


Asp Thr Arg Tyr Ile Ser Lys Tyr Ile Ser Gly Val Leu Ser Asn Ile 
                965                 970                 975     


Val Arg Thr Glu Asp Gly Thr Asp Asp Gly Val Asn Ser Lys Asn Ile 
            980                 985                 990         


Val Pro Gly Asn Gly Lys Ile Thr  Asn Thr Leu Lys Gln  Asp Trp Gly 
        995                 1000                 1005             


Leu Asn  Asp Ile Trp Asn Asp  Leu Ile Leu Pro Arg  Phe Glu Arg 
    1010                 1015                 1020             


Met Asn  Gln Leu Thr Asn Ser  Thr Asp Phe Thr Ala  Trp Asn Glu 
    1025                 1030                 1035             


Asn His  Gln Lys Phe Leu Pro  Thr Val Pro Val Glu  Phe Ser Lys 
    1040                 1045                 1050             


Gly Phe  Ser Lys Lys Arg Ile  Asp His Arg His His  Ala Leu Asp 
    1055                 1060                 1065             


Ala Leu  Val Ile Ala Cys Ala  Thr Lys Asp His Val  Asn Leu Leu 
    1070                 1075                 1080             


Asn Asn  Gln Ser Ala Lys Ser  Asp Thr Lys Arg His  Asp Leu Lys 
    1085                 1090                 1095             


Arg Lys  Leu Met Lys Phe Glu  Lys Val Ala Tyr Asn  His Pro Gln 
    1100                 1105                 1110             


Thr Gly  Glu Arg Ile Glu Arg  Glu Val Pro Lys Gln  Phe Leu Lys 
    1115                 1120                 1125             


Pro Trp  Glu Thr Phe Thr Val  Glu Ala Lys Asn Gly  Leu Glu Thr 
    1130                 1135                 1140             


Ile Ile  Val Ser Phe Lys Gln  Asn Leu Arg Val Ile  Asn Lys Ala 
    1145                 1150                 1155             


Thr Asn  His Tyr Glu Lys Tyr  Val Glu Lys Asp Gly  Ala Lys Val 
    1160                 1165                 1170             


Lys Gly  Arg Val Glu Gln Ala  Gly Thr Asn Trp Ala  Ile Arg Lys 
    1175                 1180                 1185             


Pro Met  His Lys Glu Thr Val  Ser Gly Lys Ile Asp  Leu Pro Arg 
    1190                 1195                 1200             


Ile Lys  Val Pro Lys Gly Lys  Ile Leu Thr Ala Thr  Arg Lys Asn 
    1205                 1210                 1215             


Leu Asp  Thr Ser Phe Asp Leu  Lys Thr Ile Gly Ser  Ile Thr Asp 
    1220                 1225                 1230             


Thr Gly  Ile Gln Lys Ile Leu  Lys Asn Tyr Leu Gln  Tyr Lys Asp 
    1235                 1240                 1245             


Asn Asn  Pro Glu Leu Ala Phe  Ser Pro Glu Gly Ile  Glu Asp Met 
    1250                 1255                 1260             


Asn Lys  Asn Ile Glu Lys Phe  Asn Asp Gly Lys Val  His Gln Pro 
    1265                 1270                 1275             


Ile Ile  Lys Val Arg Ile Phe  Glu Leu Gly Ser Lys  Phe Pro Leu 
    1280                 1285                 1290             


Gly Gln  Ser Gly Asn Lys Lys  Asp Lys Tyr Val Glu  Ala Ala Lys 
    1295                 1300                 1305             


Gly Thr  Asn Leu Phe Phe Ala  Val Tyr Glu Asp Lys  Asn Gly Lys 
    1310                 1315                 1320             


Arg Ser  Tyr Glu Thr Ile Pro  Leu Asn Glu Val Ile  Glu Arg Gln 
    1325                 1330                 1335             


Lys Gln  Gly Leu Thr Val Val  Asp Leu Lys Gly Val  Asn Asp Phe 
    1340                 1345                 1350             


Tyr Leu  Ser Pro Asn Asp Leu  Val Tyr Val Pro Ser  Glu Asp Glu 
    1355                 1360                 1365             


Leu Glu  Asn Val Ser Met Ile  Asp Tyr Asn Lys Leu  Ser Asn Glu 
    1370                 1375                 1380             


Lys Val  Tyr Lys Val Val Ser  Phe Thr Gly Asn Gln  Ile Phe Phe 
    1385                 1390                 1395             


Val Arg  Gln Asp Val Ala Thr  Ser Ile Val Asn Lys  Ala Glu Phe 
    1400                 1405                 1410             


Ser Ser  Leu Asn Lys Met Glu  Arg Ala Ile Gly Gly  Thr Met Ile 
    1415                 1420                 1425             


Lys Glu  Val Phe Val Lys Ile  Lys Ile Asp Arg Leu  Gly Asn Ile 
    1430                 1435                 1440             


Ser Lys  Val 
    1445     


<210>  1264
<211>  1448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1264

Met Lys His Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Lys Glu Ala Gln Asn Asp Asn Glu Gln Ser Glu Ile Val 
            20                  25                  30          


Asp Val Gly Val Arg Val Asn Pro Leu Thr Thr Asp Glu Lys Thr Asn 
        35                  40                  45              


Phe Glu Lys Gly Lys Pro Ile Ser Thr Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  


Arg Ser Ala Arg Arg Asn Leu Gln Arg Tyr Lys Leu Arg Arg Lys Glu 
65                  70                  75                  80  


Leu Ile Glu Leu Leu Lys Glu His Gln Leu Ile Ser Glu Asn Thr Ile 
                85                  90                  95      


Leu Thr Glu Asn Gly Lys Gly Thr Thr Phe Gln Thr Leu Glu Leu Arg 
            100                 105                 110         


Ala Lys Ser Ala Lys Glu Lys Ile Ser Leu Gly Glu Leu Ala Arg Val 
        115                 120                 125             


Phe Leu Thr Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 


Lys Asn Gly Asp Glu Gly Gln Leu Ile Asp Gly Met Glu Ile Ala Lys 
145                 150                 155                 160 


Arg Leu Tyr Glu Glu Asn Leu Thr Thr Gly Gln Leu Ser Leu Gln Leu 
                165                 170                 175     


Leu Lys Glu Gly Lys Lys Ser Leu Pro Asp Tyr Tyr Arg Ser Asp Leu 
            180                 185                 190         


Gln Phe Glu Leu Asp Lys Val Trp Asn Phe Gln Lys Gln Ser His Ser 
        195                 200                 205             


Asp Ile Leu Thr Asp Glu Phe Tyr Ala Gln Leu Lys Gly Lys Gly Gln 
    210                 215                 220                 


Arg Ala Thr Ser Ala Leu Phe Leu Ala Lys Tyr Gln Ile Tyr Thr Ala 
225                 230                 235                 240 


Glu Asn Lys Gly Ser Arg Glu Asp Lys Lys Leu Gln Ala Tyr Gln Trp 
                245                 250                 255     


Arg Val Asp Ala Leu Ser Lys Glu Leu Thr Arg Glu Glu Leu Ala Phe 
            260                 265                 270         


Val Ile Thr Glu Ile Asn Asn Asn Leu Asn Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             


Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Asn Glu Thr 
    290                 295                 300                 


Val Gly Glu Tyr Leu Trp Lys Gln Ile Ala Ala Asn Pro His Asn Ser 
305                 310                 315                 320 


Leu Lys Asn Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     


Gln Ile Trp Lys Thr Gln Ser Ala Phe Tyr Pro Glu Ile Leu Thr Asp 
            340                 345                 350         


Glu Leu Lys Lys Glu Leu Arg Asp Val Val Ile Phe Tyr Gln Arg Lys 
        355                 360                 365             


Leu Lys Ser Gln Lys Ser Leu Val Ser Phe Cys Glu Phe Glu Asn Lys 
    370                 375                 380                 


Glu Ile Glu Ile Lys Asn Glu Asp Gly Thr Thr Thr Lys Lys Val Ile 
385                 390                 395                 400 


Gly Leu Lys Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe Lys 
                405                 410                 415     


Ile Trp Gln Asn Leu Asn Asn Val Leu Ile Arg Lys Lys Gly Ser Lys 
            420                 425                 430         


Thr Lys Lys Val Ala Lys Asn Gln Gln Ala Val Leu Phe Glu Glu Glu 
        435                 440                 445             


Lys Thr Ile Ser Glu Leu Asp Leu Glu Ala Lys Gln Ala Leu Phe Asn 
    450                 455                 460                 


Glu Leu Asn Leu Lys Gly Asn Leu Lys Ala Asp Tyr Cys Leu Glu Ile 
465                 470                 475                 480 


Leu Gly Tyr Ser Pro Lys Glu Trp Glu Met Asn Tyr Ser Ile Leu Glu 
                485                 490                 495     


Gly Asn Arg Thr Asn Lys Ala Leu Tyr Glu Ala Tyr Leu Lys Ile Thr 
            500                 505                 510         


Asp Leu Glu Gly Tyr Asp Ala Lys Asn His Leu Lys Val Lys Leu Asn 
        515                 520                 525             


Lys Asp Glu Val Glu Leu Asp Asp Leu Lys Val Pro Ala Ser Glu Ile 
    530                 535                 540                 


Lys Asp Met Val Gln Ala Ile Phe Lys Glu Ala Gly Ile Asn Thr Asp 
545                 550                 555                 560 


Ile Leu His Phe Asn Ala Glu Leu Asp Gly Lys Asp Phe Glu Asn Gln 
                565                 570                 575     


Ala Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp Asp 
            580                 585                 590         


Ser Lys Ser Gly Asn Glu Leu Leu Tyr Lys Leu Leu Glu Thr Lys Phe 
        595                 600                 605             


Gly Phe Lys Lys Glu Tyr Ala Gln Ile Leu Ala Asn Val Ser Leu Ser 
    610                 615                 620                 


Asp Asp Tyr Gly Asn Leu Ser Thr Lys Ala Met Arg Glu Ile Ile Thr 
625                 630                 635                 640 


His Leu Lys Gln Gly Phe Pro Phe Thr Gly Arg Lys Asp Arg Pro Asp 
                645                 650                 655     


Glu Pro Ser Ala Cys Glu Phe Ala Gly Tyr Lys His Ser Lys His Ser 
            660                 665                 670         


Leu Thr Lys Glu Glu Asn Glu Lys Arg Val Leu Lys Asp Ser Leu Glu 
        675                 680                 685             


Val Leu Lys Lys Asn Ser Leu Arg Gln Pro Val Val Glu Lys Ile Leu 
    690                 695                 700                 


Asn Gln Met Ile Asn Leu Val Asn Ala Leu Leu Lys Lys Tyr Arg Thr 
705                 710                 715                 720 


Glu Asn Pro Asn Phe Lys Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg 
                725                 730                 735     


Glu Leu Lys Lys Lys Ala Glu Glu Arg Ala Asn Met Thr Ala Asn Ile 
            740                 745                 750         


Asn Lys Ala Lys Thr Glu His Glu Lys Ile Ile Lys Ile Leu Lys Asn 
        755                 760                 765             


Glu Phe Gly Leu Pro Asn Pro Thr Arg Asn Asp Ile Ile Arg Tyr Arg 
    770                 775                 780                 


Leu Tyr Glu Glu Leu Lys Asp Asn Gly Tyr Lys Asp Leu Tyr Lys Gly 
785                 790                 795                 800 


Asp Tyr Ile Pro Arg Glu Lys Leu Phe Ser Lys Glu Ile Asp Ile Glu 
                805                 810                 815     


His Ile Ile Pro Lys Ala Arg Val Phe Asp Asp Ser Phe Ser Asn Lys 
            820                 825                 830         


Thr Ile Thr Tyr Arg Lys Thr Asn Leu Glu Lys Gly Glu Arg Thr Ala 
        835                 840                 845             


Tyr Asp Tyr Ile Glu Ser Leu Gly Glu Lys Gln Leu Glu Glu Phe Leu 
    850                 855                 860                 


Ser Arg Ile Glu Asn Leu Tyr Glu Ile Gly Lys Pro Ser Arg Leu His 
865                 870                 875                 880 


Asn Arg Arg Asn Pro Leu Lys Lys Val Glu Glu Lys Gly Ile Ser Asp 
                885                 890                 895     


Ser Lys Tyr Lys Lys Leu Leu Lys Lys Glu Ser Glu Ile Gly Asp Gly 
            900                 905                 910         


Phe Val Glu Arg Asp Leu Arg Glu Thr Gln Tyr Ile Ala Lys Lys Ala 
        915                 920                 925             


Lys Glu Ile Leu Leu Gln Ile Thr Arg Ser Val Leu Ser Thr Ser Gly 
    930                 935                 940                 


Gly Ile Thr Asp Arg Leu Arg Glu Asp Trp Asp Leu Ile Asn Val Met 
945                 950                 955                 960 


Lys Glu Leu Asn Leu Pro Lys Tyr Arg Ala Leu Gly Leu Thr Lys Met 
                965                 970                 975     


Glu Asp Arg Lys Tyr Gly Gln Gln Val Glu Val Ile Thr Asp Trp Thr 
            980                 985                 990         


Lys Arg Asn Asp Leu Arg His His  Ala Met Asp Ala Leu  Thr Val Ala 
        995                 1000                 1005             


Phe Thr  Lys His Asn His Ile  Gln Tyr Leu Asn Tyr  Leu Asn Ala 
    1010                 1015                 1020             


Arg Lys  Asn Glu Lys His Lys  Glu His Asn Asn Ile  Met Ala Ile 
    1025                 1030                 1035             


Gln Gln  Leu Glu Thr Ile Lys  Val Thr Asp Glu Asn  Gly Asn Glu 
    1040                 1045                 1050             


Lys Arg  Ile Phe Lys Ala Pro  Met Pro Asn Phe Arg  Gln Val Ala 
    1055                 1060                 1065             


Lys Ala  Phe Leu Glu Asn Val  Leu Ile Ser His Lys  Ala Lys Asn 
    1070                 1075                 1080             


Lys Val  Val Thr Lys Asn Lys  Asn Lys Ile Ser Gly  Asn Asn Lys 
    1085                 1090                 1095             


Val Gln  Glu Val Leu Thr Pro  Arg Gly Gln Leu His  Lys Glu Thr 
    1100                 1105                 1110             


Ile Tyr  Gly Lys Tyr Gln Tyr  Tyr Val Gln Lys Glu  Glu Lys Ile 
    1115                 1120                 1125             


Gly Gly  Lys Phe Asp Leu Glu  Thr Ile Glu Lys Val  Ala Asn Pro 
    1130                 1135                 1140             


Leu Tyr  Gln Ser Leu Leu Lys  Lys Arg Leu Glu Glu  Asn Gly Gly 
    1145                 1150                 1155             


Asp Pro  Lys Lys Ala Phe Thr  Gly Lys Asn Ser Leu  Ala Lys Asn 
    1160                 1165                 1170             


Pro Ile  Tyr Leu Asp Glu Ala  Gln Thr Glu Gln Leu  Pro Glu Lys 
    1175                 1180                 1185             


Val Lys  Leu Val Trp Leu Glu  Asn Ser Phe Ser Ile  Arg Lys Asp 
    1190                 1195                 1200             


Ile Thr  Pro Glu Asn Phe Lys  Asp Glu Lys Leu Ile  Asp Lys Ile 
    1205                 1210                 1215             


Leu Asp  Glu Gly Val Lys Arg  Ile Leu Lys Glu Arg  Leu Gln Ser 
    1220                 1225                 1230             


Phe Gly  Asn Asp Ala Lys Lys  Ala Phe Ser Asp Leu  Glu Lys Asn 
    1235                 1240                 1245             


Pro Ile  Trp Leu Asn Lys Glu  Lys Gly Ile Ala Ile  Lys Arg Val 
    1250                 1255                 1260             


Thr Ile  Ser Gly Val Ala Asn  Ala Glu Pro Leu His  Thr Lys Lys 
    1265                 1270                 1275             


Asp His  Leu Gly Lys Glu Ile  Leu Asp Lys Glu Gly  Lys Pro Ile 
    1280                 1285                 1290             


Pro Val  Asp Phe Ile Ser Thr  Gly Ser Asn His His  Val Ala Val 
    1295                 1300                 1305             


Tyr Arg  Asp Glu Lys Gly Asn  Leu Gln Glu Lys Val  Val Ser Val 
    1310                 1315                 1320             


Phe Glu  Ala Val Val Arg Ala  Asn Gln Gly Leu Pro  Ile Ile Asp 
    1325                 1330                 1335             


Lys Thr  Tyr Asn Gln Tyr Leu  Gly Trp Glu Phe Leu  Phe Ser Met 
    1340                 1345                 1350             


Lys Gln  Asn Glu Met Phe Val  Phe Pro Asn Glu Ser  Thr Gly Phe 
    1355                 1360                 1365             


Asn Pro  Asn Asp Ile Asp Leu  Leu Asp Pro Lys Asn  Lys Lys Ile 
    1370                 1375                 1380             


Ile Ser  Pro Asn Leu Phe Arg  Val Gln Lys Leu Ala  Thr Gly Asp 
    1385                 1390                 1395             


Tyr Trp  Phe Arg His His Leu  Glu Thr Thr Val Glu  Thr Lys Ser 
    1400                 1405                 1410             


Ala Thr  Lys Asp Met Thr Tyr  Lys Arg Leu Ser Leu  Asn His Ile 
    1415                 1420                 1425             


Lys Asn  Ile Ile Lys Val Arg  Ile Asn His Leu Gly  Asp Ile Val 
    1430                 1435                 1440             


Ser Val  Gly Glu Tyr 
    1445             


<210>  1265
<211>  1450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1265

Met Lys His Ile Leu Gly Leu Asp Leu Gly Ser Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Val Gln Gln Asp Phe Glu Asn Lys Gln Gly Lys Ile Ile Ala 
            20                  25                  30          


Thr Gly Ser Arg Ile Ile Pro Met Asp Gln Gly Ile Leu Gly Asp Phe 
        35                  40                  45              


Glu Arg Gly Asn Thr Val Ser Gln Thr Ala Glu Arg Thr Thr Tyr Arg 
    50                  55                  60                  


Asn Thr Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Ile Leu Gly Phe Leu Pro Pro His Tyr Asp Ser 
                85                  90                  95      


Gln Ile Asp Phe Thr Lys Arg Tyr Gly Lys Phe Ile Asp Asn Ala Glu 
            100                 105                 110         


Pro Lys Ile Ala Tyr Asn Asn Gly Asn Phe Ile Phe Met Asn Ser Phe 
        115                 120                 125             


Asn Glu Met Ile Glu Asp Phe Lys Lys Tyr Gln Pro Gln Leu Phe Tyr 
    130                 135                 140                 


Lys Lys Ser Asn Gly Glu Glu Ser Lys Ile Pro Tyr Asp Trp Thr Ile 
145                 150                 155                 160 


Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Lys Ile Thr Gln Gln Glu 
                165                 170                 175     


Leu Ala Trp Leu Ile Leu His Phe Asn Gln Lys Arg Gly Tyr Tyr Gln 
            180                 185                 190         


Leu Arg Gly Glu Glu Glu Thr Glu Asn Pro Asn Lys Glu Val Ala Phe 
        195                 200                 205             


His Ser Leu Glu Val Val Asp Val Glu Ala Glu Ala Pro Asn Lys Lys 
    210                 215                 220                 


Gly Glu Ile Trp Tyr Thr Ile Arg Leu Glu Asn Gly Trp Ile Tyr Arg 
225                 230                 235                 240 


Arg Thr Ser Lys Ile Pro Leu Asp Asp Trp Lys Gly Lys Thr Arg Asp 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Asn Asp Asp Gly Thr Ile Lys Leu Asp 
            260                 265                 270         


Lys Glu Gly Lys Glu Lys Arg Ser Phe Arg Ala Pro Asp Glu Asn Asp 
        275                 280                 285             


Trp Asn Leu Leu Lys Lys Lys Thr Glu His Asp Ile Asp Phe Ser Gly 
    290                 295                 300                 


Lys Thr Val Gly Ala Tyr Ile Tyr Asp His Leu Leu Gln Asn Pro Asn 
305                 310                 315                 320 


Gln Lys Ile Lys Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Asp Glu Leu Lys Ala Ile Leu Ala Lys Gln Ile Ala Leu Gln Pro 
            340                 345                 350         


Glu Leu Phe Thr Glu Thr Leu Leu Ala Asp Cys Ile Arg Glu Leu Tyr 
        355                 360                 365             


Arg Lys Asn Glu Thr Gln Gln Gln Asn Leu Leu Ser Lys Asp Phe Leu 
    370                 375                 380                 


His Leu Phe Val Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser 
385                 390                 395                 400 


Lys Lys Ser Thr Ile Ala Asn Cys Thr Leu Glu Lys Arg Ser Tyr Ile 
                405                 410                 415     


Asp Lys Glu Thr Asn Glu Arg Lys Asp Thr Pro Ile Lys Val Cys Ala 
            420                 425                 430         


Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Val Leu Gln Trp Leu Gln 
        435                 440                 445             


Asn Leu Arg Ile Tyr Glu Ile Glu Thr Asp Arg Glu Val Thr Ala Glu 
    450                 455                 460                 


Phe Ile Glu Thr Ala Gln Asp Asn Glu Ala Leu Phe Asn Phe Leu Met 
465                 470                 475                 480 


Ser Gln Lys Glu Ile Asp Asn Glu Ser Leu Leu Lys Tyr Phe Leu Phe 
                485                 490                 495     


Ala Lys Asn Pro Gln Ile Lys Asp Lys Val Ala Lys Ser Glu Leu Lys 
            500                 505                 510         


Lys Trp Leu Thr Thr Tyr Arg Trp Asn Tyr Val Tyr Asp Ala Ala Asp 
        515                 520                 525             


Asn Ser Ser Lys Lys Tyr Pro Met Asn Glu Thr Gly Tyr Glu Leu Gln 
    530                 535                 540                 


Gln Tyr Leu Gln Lys Val Glu Gly Leu Pro Ala Asn Phe Leu Thr Phe 
545                 550                 555                 560 


Asp Ile Thr Tyr Arg Leu Trp His Leu Leu Tyr Ser Val Lys Asp Lys 
                565                 570                 575     


Val Glu Phe Glu Lys Ala Leu Lys Lys Phe Ala Gln Glu Tyr Gln Leu 
            580                 585                 590         


Asp Glu Ala Ser Phe Val Asp Ser Phe Lys Asn Phe Lys Pro Tyr Pro 
        595                 600                 605             


Asn Glu Tyr Gly Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro 
    610                 615                 620                 


Leu Met Arg Phe Gly Asn His Trp Asp Phe Asn Arg Ile Asp Val Ser 
625                 630                 635                 640 


Thr Gln Lys Arg Ile Asp Asp Leu Ile Asn Gly Val Ala Asn Asp Glu 
                645                 650                 655     


Ile Arg Thr Ile Ile Arg Glu Lys Ala Glu Lys Tyr Gln Leu Asn Ser 
            660                 665                 670         


Glu Thr Cys Phe Gln Gly Leu Pro Leu Trp Leu Ala Gln Tyr Val Val 
        675                 680                 685             


Tyr Asn Arg His Ala Glu Ala Ala Asp Ile Glu Lys Trp Thr Ser Val 
    690                 695                 700                 


Asn Asp Leu Glu Ala Tyr Leu Glu Asp Phe Lys Gln His Ser Leu Arg 
705                 710                 715                 720 


Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val Val Arg 
                725                 730                 735     


Asp Ile Trp Gln Arg Tyr Gly Gly Gly Gln Ala Asn Phe Phe Asp Glu 
            740                 745                 750         


Ile His Ile Glu Leu Gly Arg Glu Leu Lys Lys Thr Ala Glu Glu Arg 
        755                 760                 765             


Lys Gln Leu Ser Asn Arg Asn Gln Glu Asn Glu Asn Thr Asn Leu Arg 
    770                 775                 780                 


Ile Lys Lys Leu Leu Ala Glu Leu Lys Glu Asp Ala Ser Ile Gln Asn 
785                 790                 795                 800 


Val Arg Pro Tyr Ser Pro Val Gln Gln Glu Leu Leu Lys Ile Tyr Glu 
                805                 810                 815     


Glu Gly Val Phe Ser Ala Thr Asp Glu Val Glu Glu Asp Ile Gln Lys 
            820                 825                 830         


Ile Arg Lys Lys Ala Glu Pro Thr Thr Pro Glu Ile Gln Arg Tyr Lys 
        835                 840                 845             


Leu Trp Leu Glu Gln Lys Tyr Lys Ser Pro Tyr Thr Gly Glu Val Ile 
    850                 855                 860                 


Ser Leu Thr Lys Leu Phe Thr Glu Glu Tyr Glu Ile Glu His Ile Ile 
865                 870                 875                 880 


Pro Gln Ser Arg Tyr Tyr Asp Asp Ser Leu Ser Asn Lys Val Ile Cys 
                885                 890                 895     


Glu Ser Ala Val Asn Lys Leu Lys Thr Asn Gln Leu Gly Leu Glu Phe 
            900                 905                 910         


Ile Gln Asn His His Gly Glu Lys Val Arg Ile Thr Ala Asn Lys Glu 
        915                 920                 925             


Val Thr Ile Phe Thr Glu Glu Gln Tyr Lys Glu Phe Val Lys Lys His 
    930                 935                 940                 


Tyr Ala Asn Asn Ser Ala Lys Gln Gln Lys Leu Leu Leu Asn Glu Ile 
945                 950                 955                 960 


Pro Glu Lys Met Val Ala Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser 
                965                 970                 975     


Lys Tyr Ile Ser Glu Ile Leu Ser Lys Ile Val Arg Ser Asp Glu Lys 
            980                 985                 990         


Asp Glu Gly Val Asn Ser Lys Asn  Ile Ile Pro Cys Thr  Gly Lys Ile 
        995                 1000                 1005             


Thr Thr  Ala Leu Lys Gln Asp  Trp Gly Leu Asn Asp  Val Trp Asn 
    1010                 1015                 1020             


Glu Leu  Ile Leu Pro Arg Phe  Glu Arg Met Asn Gln  Leu Thr Gln 
    1025                 1030                 1035             


Thr Gln  Leu Phe Thr Ser Tyr  Asn Asp Arg Phe Gln  Lys Asn Leu 
    1040                 1045                 1050             


Pro Thr  Val Pro Ile Glu Tyr  Ser Gln Gly Phe Gln  Lys Lys Arg 
    1055                 1060                 1065             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1070                 1075                 1080             


Ala Thr  Arg Glu His Ile Asn  Tyr Met Asn Asn Gln  His Ala Leu 
    1085                 1090                 1095             


Asp Lys  Ser Lys Asp Lys Lys  Glu Lys Gln Gln Ser  Arg Ile Asp 
    1100                 1105                 1110             


Leu Arg  Thr Ile Leu Cys Glu  Lys Lys Asn Asn Asn  Trp Gln Phe 
    1115                 1120                 1125             


Lys Lys  Pro Trp Ser Thr Phe  Thr Gln Glu Ala Gln  Glu Ala Leu 
    1130                 1135                 1140             


Glu His  Ile Val Val Ser Phe  Lys Gln Asn Leu Arg  Val Ile Asn 
    1145                 1150                 1155             


Lys Ala  Thr Asn Tyr Tyr Glu  Lys Trp Glu Glu Lys  Asn Gly Val 
    1160                 1165                 1170             


Leu Val  Lys Lys Lys Val Lys  Gln Glu Gly Thr Asn  Trp Ala Ile 
    1175                 1180                 1185             


Arg Lys  Pro Leu His Lys Glu  Thr Val Ser Gly Lys  Ile Val Leu 
    1190                 1195                 1200             


Asp Arg  Lys Ile Gly Lys Asp  Lys Ile Leu Thr Ala  Thr Arg Lys 
    1205                 1210                 1215             


Ser Val  Asp Ile Thr Phe Thr  Glu Lys Ile Ile Ser  Ser Ile Thr 
    1220                 1225                 1230             


Asp Thr  Gly Ile Gln Lys Ile  Leu Leu Asn Tyr Leu  Lys Tyr Lys 
    1235                 1240                 1245             


Gly Ser  Pro Glu Val Ala Phe  Ser Ala Glu Gly Leu  Glu Glu Leu 
    1250                 1255                 1260             


Asn Lys  Asn Ile Ala Gln Tyr  Asn Asp Gly Lys Lys  His Gln Pro 
    1265                 1270                 1275             


Ile Tyr  Lys Val Arg Ile Phe  Glu Glu Gly Ser Lys  Phe Pro Leu 
    1280                 1285                 1290             


Gly Glu  Thr Gly Ala Lys Ala  Thr Lys Tyr Val Glu  Ala Ala Lys 
    1295                 1300                 1305             


Gly Thr  Asn Leu Phe Phe Gly  Val Tyr Gln Gly Lys  Gly Lys Arg 
    1310                 1315                 1320             


Thr Tyr  Ala Thr Ile Pro Leu  Asn Glu Val Ile Glu  Arg Gln Lys 
    1325                 1330                 1335             


Gln Gly  Leu Pro Ser Val Pro  Glu Arg Asn Glu Lys  Gly Glu Pro 
    1340                 1345                 1350             


Leu Leu  Phe Ser Leu Ser Pro  Asn Asp Leu Val Tyr  Val Pro Met 
    1355                 1360                 1365             


Glu Gly  Glu Ile Ala Glu Thr  Ile Asp Phe Asn Asn  Leu Ser Lys 
    1370                 1375                 1380             


Glu Gln  Lys Glu Arg Ile Tyr  Lys Thr Val Ser Phe  Thr Gly Asn 
    1385                 1390                 1395             


Gln Cys  Phe Phe Val Gln Glu  Ala Val Ala Thr Pro  Ile Val Asn 
    1400                 1405                 1410             


Lys Ile  Glu Tyr Ser Pro Leu  Asn Lys Met Glu Arg  Asp Ile Leu 
    1415                 1420                 1425             


Gly Thr  Met Ile Lys Glu Val  Cys Ile Lys Leu Lys  Val Asp Arg 
    1430                 1435                 1440             


Leu Gly  Asn Ile Thr Lys Ala  
    1445                 1450 


<210>  1266
<211>  1450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1266

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Ser Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Val Glu Gln Asp Phe Glu Asn Lys Gln Gly Lys Ile Ile Ala 
            20                  25                  30          


Thr Gly Ser Arg Ile Ile Pro Met Asp Gln Gly Ile Leu Gly Asp Phe 
        35                  40                  45              


Glu Lys Gly Asn Thr Val Ser Gln Thr Ala Glu Arg Thr Thr Tyr Arg 
    50                  55                  60                  


Ser Met Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Ile Leu Gly Phe Leu Pro Pro His Tyr Asp Ala 
                85                  90                  95      


Gln Ile Asp Phe Thr Lys Arg Tyr Gly Lys Phe Ile Asp Asn Ala Glu 
            100                 105                 110         


Pro Lys Ile Ala Tyr Asn Asn Gly Asn Phe Ile Phe Met Asn Ser Phe 
        115                 120                 125             


Asn Glu Met Ile Glu Asp Phe Lys Lys Tyr Gln Pro Gln Leu Phe Tyr 
    130                 135                 140                 


Lys Lys Ser Asn Gly Glu Glu Ser Lys Ile Pro Tyr Asp Trp Thr Ile 
145                 150                 155                 160 


Tyr Tyr Leu Arg Lys Lys Val Leu Ser Gln Lys Ile Thr Gln Gln Glu 
                165                 170                 175     


Leu Ala Trp Leu Ile Leu His Phe Asn Gln Lys Arg Gly Tyr Tyr Gln 
            180                 185                 190         


Leu Arg Gly Glu Glu Glu Thr Glu Asn Pro Asn Lys Glu Val Ala Phe 
        195                 200                 205             


His Ser Leu Lys Val Val Asp Val Glu Ala Glu Ala Pro Asn Lys Lys 
    210                 215                 220                 


Gly Glu Ile Trp Tyr Thr Ile Arg Leu Glu Asn Gly Trp Ile Tyr Arg 
225                 230                 235                 240 


Arg Thr Ser Lys Ile Pro Leu Asp Asp Trp Lys Gly Lys Thr Arg Asp 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Asn Asp Asp Gly Thr Ile Lys Val Asp 
            260                 265                 270         


Lys Glu Gly Lys Glu Lys Arg Ser Phe Arg Ala Pro Asp Glu Asn Asp 
        275                 280                 285             


Trp Asn Leu Leu Lys Lys Lys Thr Glu His Asp Ile Asp Phe Ser Gly 
    290                 295                 300                 


Lys Thr Val Gly Ala Tyr Ile Tyr Asp His Leu Leu Gln Asn Pro Asn 
305                 310                 315                 320 


Gln Lys Ile Lys Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Asp Glu Leu Lys Ala Ile Leu Ala Lys Gln Ile Ala Leu Gln Pro 
            340                 345                 350         


Glu Leu Phe Thr Glu Thr Leu Leu Ala Asp Cys Ile Arg Glu Leu Tyr 
        355                 360                 365             


Arg Lys Asn Glu Thr Gln Gln Gln Asn Leu Leu Ser Lys Asp Phe Leu 
    370                 375                 380                 


His Leu Phe Val Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser 
385                 390                 395                 400 


Lys Lys Ser Thr Ile Ala Asn Cys Thr Leu Glu Lys Arg Ser Tyr Ile 
                405                 410                 415     


Asp Lys Glu Thr Asn Glu Arg Lys Asp Thr Phe Ile Lys Val Cys Ala 
            420                 425                 430         


Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Val Leu Gln Trp Leu Gln 
        435                 440                 445             


Asn Leu Arg Ile Tyr Glu Ile Glu Thr Asp Arg Glu Val Thr Ala Glu 
    450                 455                 460                 


Phe Ile Glu Thr Ala Glu Asp Tyr Glu Ala Leu Phe Asn Phe Leu Met 
465                 470                 475                 480 


Ser Gln Lys Glu Ile Asp Asn Glu Gly Leu Leu Lys Tyr Phe Leu Phe 
                485                 490                 495     


Ala Lys Asn Pro Gln Ile Lys Asp Lys Val Ala Lys Ser Glu Leu Lys 
            500                 505                 510         


Lys Trp Leu Thr Thr Tyr Arg Trp Asn Tyr Val Tyr Asp Ala Ala Asp 
        515                 520                 525             


Asn Ser Ser Lys Lys Tyr Pro Met Asn Glu Thr Gly Tyr Asp Leu Gln 
    530                 535                 540                 


Arg Tyr Leu Gln Lys Val Glu Gly Leu Pro Ala Asn Phe Leu Thr Phe 
545                 550                 555                 560 


Asp Ile Thr Tyr Arg Leu Trp His Leu Leu Tyr Ser Val Lys Asp Lys 
                565                 570                 575     


Val Glu Phe Glu Lys Ala Leu Lys Lys Phe Ala Gln Glu Tyr Gln Leu 
            580                 585                 590         


Asp Glu Ala Ser Phe Val Asp Ser Phe Lys Asn Phe Lys Pro Tyr Pro 
        595                 600                 605             


Asn Glu Tyr Gly Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro 
    610                 615                 620                 


Leu Met Arg Phe Gly Asn His Trp Asp Phe Asn Arg Ile Asp Val Ser 
625                 630                 635                 640 


Thr Gln Lys Arg Ile Asp Asp Leu Ile Asn Gly Val Ala Asn Asp Glu 
                645                 650                 655     


Ile Arg Thr Ile Ile Arg Glu Lys Ala Glu Lys Tyr Gln Leu Asn Ser 
            660                 665                 670         


Glu Ser Cys Phe Gln Gly Leu Pro Leu Trp Leu Ala Gln Tyr Val Val 
        675                 680                 685             


Tyr Asn Arg His Ala Glu Ala Thr Asp Ile Glu Lys Trp Thr Ser Val 
    690                 695                 700                 


Ser Asp Leu Glu Thr Tyr Leu Glu Asp Phe Lys Gln His Ser Leu Arg 
705                 710                 715                 720 


Asn Pro Ile Val Glu Gln Val Val Thr Glu Thr Leu Arg Val Val Arg 
                725                 730                 735     


Asp Ile Trp Gln Gln Tyr Gly Gly Gly Gln Ala Asn Phe Phe Asp Glu 
            740                 745                 750         


Ile His Ile Glu Leu Gly Arg Glu Leu Lys Lys Thr Ala Glu Glu Arg 
        755                 760                 765             


Lys Gln Leu Ser Asn Arg Asn Gln Glu Asn Glu Asn Thr Asn Leu Arg 
    770                 775                 780                 


Ile Lys Lys Leu Leu Ala Glu Leu Lys Glu Asp Ala Ser Ile Gln Asn 
785                 790                 795                 800 


Val Arg Pro Tyr Ser Pro Val Gln Gln Glu Leu Leu Lys Ile Tyr Glu 
                805                 810                 815     


Glu Gly Val Phe Ser Ser Thr Asp Gln Val Glu Glu Asp Ile Gln Lys 
            820                 825                 830         


Ile Arg Lys Lys Ala Glu Pro Thr Thr Pro Glu Ile Gln Arg Tyr Lys 
        835                 840                 845             


Leu Trp Leu Glu Gln Lys Tyr Lys Ser Pro Tyr Thr Gly Glu Val Ile 
    850                 855                 860                 


Ser Leu Thr Lys Leu Phe Thr Glu Glu Tyr Glu Ile Glu His Ile Ile 
865                 870                 875                 880 


Pro Gln Ser Arg Tyr Tyr Asp Asp Ser Leu Ser Asn Lys Val Ile Cys 
                885                 890                 895     


Glu Ser Ala Val Asn Lys Leu Lys Thr Asn Gln Leu Gly Leu Glu Phe 
            900                 905                 910         


Ile Gln Asn His His Gly Glu Lys Val Arg Ile Thr Gly Asn Lys Glu 
        915                 920                 925             


Val Thr Ile Phe Thr Glu Glu Gln Tyr Lys Glu Phe Val Lys Lys His 
    930                 935                 940                 


Tyr Ala Asn Asn Ser Ala Lys Gln Gln Lys Leu Leu Leu Asn Glu Ile 
945                 950                 955                 960 


Pro Glu Lys Met Val Ala Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser 
                965                 970                 975     


Lys Tyr Ile Ser Glu Ile Leu Ser Lys Ile Val Arg Ser Asp Glu Lys 
            980                 985                 990         


Asp Glu Gly Val Asn Ser Lys Asn  Val Ile Pro Cys Thr  Gly Lys Ile 
        995                 1000                 1005             


Thr Thr  Ala Leu Lys Gln Asp  Trp Gly Leu Asn Asp  Val Trp Asn 
    1010                 1015                 1020             


Glu Leu  Ile Leu Pro Arg Phe  Glu Arg Met Asn Gln  Leu Thr Gln 
    1025                 1030                 1035             


Thr Gln  Leu Phe Thr Ser Tyr  Asn Asp Arg Phe Gln  Lys Asn Leu 
    1040                 1045                 1050             


Pro Thr  Val Pro Ile Glu Tyr  Ser Gln Gly Phe Gln  Lys Lys Arg 
    1055                 1060                 1065             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1070                 1075                 1080             


Ala Thr  Arg Glu His Ile Asn  Tyr Met Asn Asn Gln  His Ala Leu 
    1085                 1090                 1095             


Asp Lys  Asn Lys Asp Lys Lys  Glu Lys Gln Gln Ser  Arg Ile Asp 
    1100                 1105                 1110             


Leu Arg  Thr Ile Leu Cys Glu  Lys Lys Asn Asn Asn  Trp Gln Phe 
    1115                 1120                 1125             


Lys Lys  Pro Trp Ser Thr Phe  Thr Gln Glu Thr Lys  Glu Ala Leu 
    1130                 1135                 1140             


Glu His  Ile Val Val Ser Phe  Lys Gln Asn Leu Arg  Val Ile Asn 
    1145                 1150                 1155             


Lys Ala  Thr Asn Tyr Tyr Glu  Lys Trp Glu Glu Lys  Asn Gly Val 
    1160                 1165                 1170             


Leu Val  Lys Lys Lys Val Lys  Gln Glu Gly Thr Asn  Trp Ala Ile 
    1175                 1180                 1185             


Arg Lys  Pro Leu His Lys Glu  Thr Val Ser Gly Lys  Ile Val Leu 
    1190                 1195                 1200             


Asp Arg  Lys Ile Gly Lys Asp  Lys Ile Leu Thr Ala  Thr Arg Lys 
    1205                 1210                 1215             


Glu Val  Asp Ile Thr Phe Thr  Glu Lys Ile Ile Ser  Ser Ile Thr 
    1220                 1225                 1230             


Asp Thr  Gly Ile Gln Lys Ile  Leu Leu Asn Tyr Leu  Asn Tyr Lys 
    1235                 1240                 1245             


Gly Ser  Pro Glu Val Ala Phe  Ser Ala Glu Gly Leu  Glu Glu Leu 
    1250                 1255                 1260             


Asn Lys  Asn Ile Ala Gln Tyr  Asn Asp Gly Lys Lys  His Gln Pro 
    1265                 1270                 1275             


Ile Tyr  Lys Val Arg Ile Phe  Glu Glu Gly Ser Lys  Phe Pro Leu 
    1280                 1285                 1290             


Gly Glu  Thr Gly Ala Lys Ala  Thr Lys Phe Val Glu  Ala Ala Lys 
    1295                 1300                 1305             


Gly Thr  Asn Leu Phe Phe Gly  Val Tyr Gln Gly Lys  Glu Lys Arg 
    1310                 1315                 1320             


Ser Tyr  Ala Thr Ile Pro Leu  Asn Glu Val Ile Glu  Arg Gln Lys 
    1325                 1330                 1335             


Gln Gly  Leu Pro Ser Val Pro  Glu Arg Asn Glu Lys  Gly Glu Pro 
    1340                 1345                 1350             


Leu Leu  Phe Ser Leu Ser Pro  Asn Asp Leu Val Tyr  Val Pro Met 
    1355                 1360                 1365             


Glu Gly  Glu Ile Ala Glu Thr  Ile Asp Phe Asn Asn  Leu Ser Lys 
    1370                 1375                 1380             


Glu Gln  Lys Glu Arg Ile Tyr  Lys Thr Val Ser Phe  Thr Gly Asn 
    1385                 1390                 1395             


Gln Cys  Phe Phe Val Gln Glu  Ala Val Ala Thr Pro  Ile Val Asn 
    1400                 1405                 1410             


Lys Met  Glu Tyr Ser Pro Leu  Asn Lys Met Glu Arg  Asp Ile Leu 
    1415                 1420                 1425             


Gly Thr  Met Ile Lys Glu Val  Cys Ile Lys Leu Lys  Val Asp Arg 
    1430                 1435                 1440             


Leu Gly  Asn Ile Thr Lys Ala  
    1445                 1450 


<210>  1267
<211>  1450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1267

Met Lys His Ile Leu Gly Leu Asp Leu Gly Ser Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Val Gln Gln Asp Phe Glu Asn Lys Gln Gly Lys Ile Ile Ala 
            20                  25                  30          


Ala Gly Ser Arg Ile Ile Pro Met Asp Gln Lys Met Leu Ser Asn Phe 
        35                  40                  45              


Glu Glu Gly Thr Thr Val Ser Gln Thr Ala Glu Arg Thr Thr Tyr Arg 
    50                  55                  60                  


Ser Met Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Ile Leu Gly Phe Leu Pro Pro His Tyr Asp Ala 
                85                  90                  95      


Gln Ile Asp Phe Thr Lys Arg Tyr Gly Lys Phe Ile Asp Asn Ala Glu 
            100                 105                 110         


Pro Lys Ile Ala Tyr Asn Asn Gly Asn Phe Ile Phe Met Asn Ser Phe 
        115                 120                 125             


Asn Glu Met Ile Glu Asp Phe Lys Glu His Gln Pro Gln Leu Phe Tyr 
    130                 135                 140                 


Lys Lys Ser Asn Gly Glu Glu Ser Lys Ile Pro Tyr Asp Trp Thr Ile 
145                 150                 155                 160 


Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Lys Ile Thr Gln Gln Glu 
                165                 170                 175     


Leu Ala Trp Leu Ile Leu His Phe Asn Gln Lys Arg Gly Tyr Tyr Gln 
            180                 185                 190         


Leu Arg Gly Glu Glu Glu Thr Glu Asn Pro Asn Lys Glu Val Ala Phe 
        195                 200                 205             


His Ser Leu Lys Val Val Asp Val Glu Ala Glu Ala Pro Asn Lys Lys 
    210                 215                 220                 


Gly Glu Ile Trp Tyr Thr Ile Arg Leu Glu Asn Gly Trp Ile Tyr Arg 
225                 230                 235                 240 


Arg Thr Ser Lys Ile Pro Leu Asp Asp Trp Lys Gly Lys Thr Arg Asp 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Asn Asp Asp Gly Thr Ile Lys Leu Asp 
            260                 265                 270         


Lys Glu Gly Lys Glu Lys Arg Ser Phe Arg Ala Pro Asp Glu Asn Asp 
        275                 280                 285             


Trp Asn Leu Leu Lys Lys Lys Thr Glu His Asp Ile Asp Phe Ser Gly 
    290                 295                 300                 


Lys Pro Val Gly Thr Tyr Ile Tyr Asp His Leu Leu Gln Asn Pro Asn 
305                 310                 315                 320 


Gln Lys Ile Lys Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Asp Glu Leu Lys Ala Ile Leu Ala Lys Gln Ile Ala Leu Gln Pro 
            340                 345                 350         


Glu Leu Phe Thr Glu Thr Leu Leu Ala Asp Cys Ile Arg Glu Leu Tyr 
        355                 360                 365             


Arg Lys Asn Glu Thr Gln Gln Gln Asn Leu Leu Ser Lys Asp Phe Leu 
    370                 375                 380                 


His Leu Phe Val Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser 
385                 390                 395                 400 


Lys Lys Ser Thr Ile Ala Asn Cys Thr Leu Glu Lys Arg Ser Tyr Ile 
                405                 410                 415     


Asp Lys Glu Thr Asn Glu Arg Lys Asp Thr Pro Ile Lys Val Cys Ser 
            420                 425                 430         


Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Val Leu Gln Trp Leu Gln 
        435                 440                 445             


Asn Leu Arg Ile Tyr Glu Ile Glu Thr Asp Arg Glu Val Thr Ala Glu 
    450                 455                 460                 


Phe Ile Glu Thr Ala Gln Asp Tyr Glu Ala Leu Phe Asn Phe Leu Met 
465                 470                 475                 480 


Ser Gln Lys Glu Ile Asp Asn Glu Ser Leu Leu Lys Tyr Phe Leu Phe 
                485                 490                 495     


Ala Lys Asn Pro Gln Ile Lys Asp Lys Thr Ala Lys Ile Glu Leu Lys 
            500                 505                 510         


Lys Trp Ile Thr Thr Tyr Arg Trp Asn Tyr Val Tyr Asp Val Ala Asp 
        515                 520                 525             


Asn Ser Ser Lys Lys Tyr Pro Met Asn Glu Thr Gly Tyr Asp Leu Gln 
    530                 535                 540                 


Arg Tyr Met Gln Lys Val Glu Gly Leu Pro Thr Asn Phe Leu Thr Phe 
545                 550                 555                 560 


Asp Thr Thr Tyr Arg Leu Trp His Leu Leu Tyr Ser Val Lys Asp Lys 
                565                 570                 575     


Val Glu Phe Glu Lys Ala Leu Lys Lys Phe Ala Lys Lys Lys Gln Leu 
            580                 585                 590         


Asp Lys Thr Ser Phe Val Asp Ser Phe Lys Asn Phe Lys Pro Tyr Pro 
        595                 600                 605             


Asn Glu Tyr Gly Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro 
    610                 615                 620                 


Leu Met Arg Phe Gly Asn His Trp Asp Phe Asn Arg Ile Asp Val Ser 
625                 630                 635                 640 


Thr Gln Lys Arg Ile Asp Asp Leu Ile Asn Gly Val Ala Asn Glu Asp 
                645                 650                 655     


Ile Arg Thr Ile Ile Arg Glu Lys Ala Glu Lys Tyr Gln Leu Asn Ser 
            660                 665                 670         


Glu Thr Cys Phe Gln Asp Leu Pro Leu Trp Leu Ala Gln Tyr Val Val 
        675                 680                 685             


Tyr Asn Arg His Ala Glu Ala Thr Asp Ile Glu Lys Trp Thr Ser Val 
    690                 695                 700                 


Ser Asp Leu Glu Ala Tyr Leu Glu Asp Phe Lys Gln His Ser Leu Arg 
705                 710                 715                 720 


Asn Pro Ile Val Glu Gln Val Val Thr Glu Thr Leu Arg Val Val Arg 
                725                 730                 735     


Asp Ile Trp Gln Gln Tyr Gly Gly Gly Gln Ala Asn Phe Phe Asp Glu 
            740                 745                 750         


Ile His Ile Glu Leu Gly Arg Glu Leu Lys Lys Thr Ala Glu Glu Arg 
        755                 760                 765             


Lys Gln Leu Ser Asp Lys Asn Gln Glu Asn Glu Asn Thr Asn Leu Arg 
    770                 775                 780                 


Ile Lys Lys Leu Leu Ala Glu Leu Lys Glu Asp Ala Ser Ile Gln Asn 
785                 790                 795                 800 


Val Arg Pro Tyr Ser Leu Val Gln Gln Glu Leu Leu Lys Ile Tyr Glu 
                805                 810                 815     


Glu Gly Val Phe Ser Ser Thr Asp Glu Ile Glu Glu Asp Ile Gln Lys 
            820                 825                 830         


Ile Arg Lys Lys Val Glu Pro Thr Thr Pro Glu Ile Gln Arg Tyr Lys 
        835                 840                 845             


Leu Trp Leu Glu Gln Lys Tyr Lys Ser Pro Tyr Thr Gly Glu Val Ile 
    850                 855                 860                 


Ser Leu Thr Lys Leu Phe Thr Glu Glu Tyr Glu Ile Glu His Ile Ile 
865                 870                 875                 880 


Pro Gln Ser Arg Tyr Tyr Asp Asp Ser Leu Ser Asn Lys Val Ile Cys 
                885                 890                 895     


Glu Ser Ala Val Asn Lys Leu Lys Thr Asn Gln Leu Gly Leu Glu Phe 
            900                 905                 910         


Ile Gln Asn His His Gly Glu Lys Val Arg Ile Thr Ala Asn Lys Glu 
        915                 920                 925             


Val Thr Ile Phe Thr Glu Glu Gln Tyr Lys Glu Phe Val Lys Lys His 
    930                 935                 940                 


Tyr Ala Asn Asn Ser Ala Lys Gln Gln Lys Leu Leu Leu Asn Glu Ile 
945                 950                 955                 960 


Pro Glu Lys Met Val Ala Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser 
                965                 970                 975     


Lys Tyr Ile Ser Glu Ile Leu Ser Lys Ile Val Arg Ser Asp Glu Lys 
            980                 985                 990         


Asp Glu Gly Val Asn Ser Lys Asn  Ile Ile Pro Cys Thr  Gly Lys Ile 
        995                 1000                 1005             


Thr Thr  Ala Leu Lys Gln Asp  Trp Gly Leu Asn Asp  Val Trp Asn 
    1010                 1015                 1020             


Glu Leu  Ile Leu Pro Arg Phe  Glu Arg Met Asn Gln  Leu Thr Gln 
    1025                 1030                 1035             


Thr Gln  Leu Phe Thr Ser Tyr  Asn Asp Arg Leu Gln  Lys Asn Leu 
    1040                 1045                 1050             


Pro Thr  Val Pro Ile Glu Tyr  Ser Gln Gly Phe Gln  Lys Lys Arg 
    1055                 1060                 1065             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1070                 1075                 1080             


Ala Thr  Arg Glu His Ile Asn  Tyr Met Asn Asn Gln  His Ala Leu 
    1085                 1090                 1095             


Asp Lys  Ser Lys Asp Lys Lys  Glu Lys Gln Gln Ser  Arg Ile Asp 
    1100                 1105                 1110             


Leu Arg  Thr Ile Leu Cys Glu  Lys Lys Asn Asn Asn  Trp Gln Phe 
    1115                 1120                 1125             


Lys Lys  Pro Trp Arg Thr Phe  Thr Gln Glu Ala Lys  Glu Ala Leu 
    1130                 1135                 1140             


Glu His  Ile Val Val Ser Phe  Lys Gln Asn Leu Arg  Val Ile Asn 
    1145                 1150                 1155             


Lys Ala  Thr Asn Tyr Tyr Glu  Lys Trp Glu Glu Lys  Asn Gly Val 
    1160                 1165                 1170             


Leu Val  Lys Lys Lys Val Lys  Gln Glu Gly Thr Asn  Trp Ala Ile 
    1175                 1180                 1185             


Arg Lys  Pro Leu His Glu Glu  Thr Phe Ser Gly Lys  Ile Ser Leu 
    1190                 1195                 1200             


Asn Arg  Glu Lys Val Gly Lys  Gly Asn Ile Leu Thr  Ala Thr Arg 
    1205                 1210                 1215             


Lys Ala  Val Asp Ser Ser Phe  Asp Val Lys Lys Ile  Ser Lys Ile 
    1220                 1225                 1230             


Thr Asp  Thr Gly Ile Gln Lys  Ile Leu Leu Asn Tyr  Leu Lys Tyr 
    1235                 1240                 1245             


Lys Gly  Ser Pro Glu Val Ala  Phe Ser Ala Glu Gly  Leu Glu Glu 
    1250                 1255                 1260             


Leu Asn  Lys Asn Ile Ala Gln  Tyr Asn Asn Gly Lys  Lys His Gln 
    1265                 1270                 1275             


Pro Ile  Tyr Lys Val Arg Ile  Tyr Glu Lys Gly Ser  Gly Arg Phe 
    1280                 1285                 1290             


Val Leu  Gly Glu Lys Gly Thr  Lys Ser Lys Lys Tyr  Val Gln Gly 
    1295                 1300                 1305             


Ala Pro  Asn Leu Tyr Phe Gly  Val Tyr Gln Gly Lys  Glu Lys Arg 
    1310                 1315                 1320             


Thr Tyr  Ala Thr Ile Pro Leu  Asn Glu Val Ile Glu  Arg Gln Lys 
    1325                 1330                 1335             


Gln Gly  Leu Pro Ser Val Pro  Glu Arg Asn Glu Lys  Gly Glu Pro 
    1340                 1345                 1350             


Leu Leu  Phe Ser Leu Ser Pro  Asn Asp Leu Val Tyr  Val Pro Met 
    1355                 1360                 1365             


Glu Gly  Glu Ala Thr Glu Thr  Ile Asn Phe Asn Asn  Leu Asn Lys 
    1370                 1375                 1380             


Glu Gln  Lys Glu Arg Ile Tyr  Lys Thr Val Ser Phe  Thr Gly Asn 
    1385                 1390                 1395             


Gln Cys  Phe Phe Val Gln Glu  Ala Val Ala Ser Pro  Ile Val Asn 
    1400                 1405                 1410             


Lys Met  Glu Tyr Ser Pro Leu  Asn Lys Met Glu Arg  Ser Ile Val 
    1415                 1420                 1425             


Gly Ile  Met Ile Lys Glu Val  Cys Ile Lys Leu Lys  Val Asp Arg 
    1430                 1435                 1440             


Leu Gly  Asn Ile Thr Lys Ala  
    1445                 1450 


<210>  1268
<211>  1453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1268

Met Lys Thr Val Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Gln His Asp Phe Asp Ser Lys Lys Gly Glu Ile Leu Gly 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Glu Phe 
        35                  40                  45              


Gly Lys Gly Asn Ser Val Ser Gln Thr Ala Asp Arg Thr Lys Phe Arg 
    50                  55                  60                  


Ser Ala Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Asn Ile Leu Gly Phe Leu Pro Arg His Tyr Ala Ala 
                85                  90                  95      


Asp Ile Asp Phe Glu Lys Arg Leu Gly Gln Phe Phe Glu Gly Lys Glu 
            100                 105                 110         


Pro Lys Leu Ala Tyr Asp Asn Asn Gln Phe Ile Phe Thr Lys Ser Phe 
        115                 120                 125             


Gly Lys Met Leu Ala Asp Phe Arg Gln His Gln Pro Asp Phe Leu Lys 
    130                 135                 140                 


Asp Glu Lys Ser Asn Asp Leu Leu Ile Pro Tyr Asp Trp Ser Ile Tyr 
145                 150                 155                 160 


Tyr Leu Arg Lys Glu Ala Leu Thr Lys Lys Ile Glu Lys Glu Glu Leu 
                165                 170                 175     


Ala Trp Ile Ile Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu 
            180                 185                 190         


Arg Gly Glu Glu Glu Glu Glu Asn Leu Asn Lys Leu Val Glu Phe His 
        195                 200                 205             


Ser Leu Lys Ile Ile Asp Val Val Ala Asp Glu Lys Leu Asn Asn Lys 
    210                 215                 220                 


Gly Glu Thr Trp Tyr Ser Leu Leu Leu Glu Asn Gly Trp Thr Tyr Arg 
225                 230                 235                 240 


Arg Ser Ser Lys Val Pro Leu Phe Asp Trp Lys Glu Lys Val Arg Asp 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Ile Asn Asp Asp Gly Ser Val Lys Thr Asp 
            260                 265                 270         


Lys Glu Gly Asn Glu Lys Arg Ser Phe Arg Ala Pro Ser Asp Asp Asp 
        275                 280                 285             


Trp Thr Leu Leu Lys Lys Lys Thr Glu Gln Asp Ile Asp Lys Ser Arg 
    290                 295                 300                 


Lys Pro Ile Gly Ala Tyr Ile Tyr Asp Ala Ile Leu Lys Asn Pro Lys 
305                 310                 315                 320 


Gln Lys Ile Asn Gly Lys Leu Val Arg Thr Val Glu Arg Lys Phe Tyr 
                325                 330                 335     


Lys Glu Glu Leu Lys Leu Ile Leu Gln Lys Gln Lys Glu Phe His Pro 
            340                 345                 350         


Glu Leu Gln Ser Thr Asp Leu Tyr Asn Glu Cys Ile Arg Glu Leu Tyr 
        355                 360                 365             


Lys Asn Asn Asp Ala His Gln Leu Gln Leu Asn Lys Lys Asp Phe Val 
    370                 375                 380                 


His Leu Phe Leu Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser 
385                 390                 395                 400 


Gln Lys Ser Ser Val Gly Asn Cys Pro Leu Glu Phe Arg Lys Phe Lys 
                405                 410                 415     


Asp Ser Glu Gly Ile Glu Lys Val Glu Tyr Leu Lys Thr Ile Pro Lys 
            420                 425                 430         


Ser Asn Pro Tyr Phe Gln Glu Phe Arg Ile Trp Gln Trp Met Tyr Asn 
        435                 440                 445             


Leu Ser Ile Tyr Lys Lys Asp Asp Asp Glu Asn Val Thr Arg Asp Phe 
    450                 455                 460                 


Leu Lys Thr Ile Glu Asp Trp Glu Asp Leu Phe Glu Phe Leu Asn His 
465                 470                 475                 480 


Arg Lys Asp Ile Glu Gln Glu Thr Leu Leu Lys Phe Leu Leu Gly Lys 
                485                 490                 495     


Asn Gly Val Lys Gly Lys Ala Leu Lys Val Glu Ala Gly Lys Phe Arg 
            500                 505                 510         


Trp Asn Tyr Val Ser Asp Lys Ile Tyr Pro Cys Asn Glu Thr Lys Ala 
        515                 520                 525             


Leu Ile Ile Ser Lys Leu Glu Lys Val Lys Gly Val Glu Glu Lys Phe 
    530                 535                 540                 


Leu Thr Glu Glu Ile Glu Tyr Lys Leu Trp His Leu Ile Tyr Ser Val 
545                 550                 555                 560 


Thr Asp Lys Val Glu Tyr Glu Lys Ala Leu Lys Ala Phe Ala Gly Lys 
                565                 570                 575     


Gln Lys Leu Asp Leu Val Ser Phe Val Asp Ser Phe Lys Arg Phe Pro 
            580                 585                 590         


Pro Phe Lys Asn Glu Tyr Gly Ala Tyr Ser Glu Lys Ala Ile Lys Lys 
        595                 600                 605             


Ile Leu Pro Leu Leu Arg Thr Gly Lys Ser Trp Asn Trp Met Ala Ile 
    610                 615                 620                 


Asp Ser Lys Val Arg Asp Arg Ile Asn Lys Ile Ile Thr Gly Glu Phe 
625                 630                 635                 640 


Asp Glu Glu Ile Lys Asn Lys Val Arg Glu Lys Ala Glu Lys His Ser 
                645                 650                 655     


Leu Lys Lys Glu Asn Asp Phe Gln Gly Leu Pro Leu Trp Leu Ala Gln 
            660                 665                 670         


Tyr Val Val Tyr Gly Arg His Ser Glu Ala Ser Phe Ser Gly Lys Trp 
        675                 680                 685             


Asn Ser Val Asp Asp Leu Lys Lys Tyr Leu Glu Glu Phe Lys Gln His 
    690                 695                 700                 


Ser Leu Arg Asn Pro Ile Val Glu Gln Val Leu Thr Glu Thr Leu Arg 
705                 710                 715                 720 


Val Val Ala Asp Ile Trp Gln Phe Tyr Gly Lys Gly Glu Lys Asp Phe 
                725                 730                 735     


Phe Ser Glu Ile His Ile Glu Leu Gly Arg Glu Met Lys Asn Thr Ala 
            740                 745                 750         


Glu Asp Arg Lys Glu Met Ser Ala Ile Ile Gln Ala Asn Glu Thr Thr 
        755                 760                 765             


Asn Leu Arg Ile Lys Ala Leu Leu Asn Glu Leu Leu Gln Asp Lys Lys 
    770                 775                 780                 


Val Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln Glu Ile Leu Lys 
785                 790                 795                 800 


Ile Tyr Glu Asp Gly Val Leu Ser Ser Asp Ile Glu Ile Pro Asp Asp 
                805                 810                 815     


Ile Gln Lys Ile Ser Lys Ala Ala Gln Pro Thr Lys Ser Glu Leu Gln 
            820                 825                 830         


Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly 
        835                 840                 845             


Ala Met Ile Pro Leu Gly Lys Leu Phe Thr Ser Glu Tyr Glu Ile Glu 
    850                 855                 860                 


His Ile Ile Pro Gln Ser Leu Tyr Phe Asp Asp Ser Phe Ser Asn Lys 
865                 870                 875                 880 


Val Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp Ser Arg Leu Gly 
                885                 890                 895     


Met Glu Phe Ile Lys Glu Cys His Gly Met Val Val Glu Thr Gly Phe 
            900                 905                 910         


Gly Lys Ser Val Thr Val Phe Glu Glu Glu Thr Tyr Arg Asp Phe Val 
        915                 920                 925             


Val Gln Asn Tyr Ser Lys Asn His Ser Lys Lys Ser Lys Leu Leu Leu 
    930                 935                 940                 


Glu Glu Ile Pro Glu Lys Met Ile Glu Arg Gln Met Asn Asp Thr Arg 
945                 950                 955                 960 


Tyr Ile Ser Lys Phe Ile Ser Ser Val Leu Ser Asn Ile Val Arg Glu 
                965                 970                 975     


Glu Val Asn Asp Asp Gly Val Asn Ser Lys Asn Ile Val Pro Gly Asn 
            980                 985                 990         


Gly Lys Ile Thr Thr Gln Leu Lys  Gln Asp Trp Gly Leu  Asn Asp Ile 
        995                 1000                 1005             


Trp Asn  Glu Leu Ile Leu Pro  Arg Phe Glu Arg Leu  Asn Val Leu 
    1010                 1015                 1020             


Thr Asp  Ser Lys His Phe Thr  Ala Trp Ser Glu Asn  His Gln Arg 
    1025                 1030                 1035             


Leu Leu  Pro Thr Val Pro Ile  Gly Leu Ser Lys Gly  Phe Ser Lys 
    1040                 1045                 1050             


Lys Arg  Ile Asp His Arg His  His Ala Leu Asp Ala  Leu Val Ile 
    1055                 1060                 1065             


Ala Cys  Ala Ser Arg Asn His  Ile Asn Phe Leu Asn  Asn Ala His 
    1070                 1075                 1080             


Ala Ile  Asp Lys Lys Lys Asn  Ser Glu Glu Lys Gln  Lys Phe Arg 
    1085                 1090                 1095             


His Asp  Leu Lys Ala Ile Leu  Cys Asp Lys Lys Tyr  Ser Asp Lys 
    1100                 1105                 1110             


Ser Glu  Lys Asn Tyr Arg Trp  Ile Phe Lys Lys Pro  Trp Asp Asn 
    1115                 1120                 1125             


Phe Thr  Ile Asp Ser Lys Asn  Ala Leu Asp Lys Ile  Ile Val Ser 
    1130                 1135                 1140             


Phe Lys  Gln Asn Leu Arg Ile  Ile Asn Lys Ala Thr  Asn Arg Tyr 
    1145                 1150                 1155             


Glu Lys  Trp Val Asp Arg Asp  Gly Ile Lys Val Lys  Glu Trp His 
    1160                 1165                 1170             


Lys Gln  Glu Gly Ile Asn Trp  Ala Thr Arg Lys Pro  Leu His Lys 
    1175                 1180                 1185             


Asp Thr  Val Ser Gly Lys Val  Asp Leu Lys Arg Val  Thr Val Pro 
    1190                 1195                 1200             


Glu Gly  Lys Ile Leu Thr Ala  Thr Arg Lys Ser Leu  Asp Thr Ser 
    1205                 1210                 1215             


Phe Asp  Leu Lys Val Ile Glu  Ser Ile Thr Asp Thr  Gly Ile Gln 
    1220                 1225                 1230             


Lys Ile  Leu Lys Asn Tyr Leu  Thr Ser Lys Ser Asn  Asn Pro Glu 
    1235                 1240                 1245             


Leu Ala  Phe Ser Ser Glu Gly  Val Glu Asp Met Asn  Lys Asn Ile 
    1250                 1255                 1260             


Arg Lys  Tyr Asn Asp Gly Lys  Leu His Gln Pro Ile  Tyr Lys Val 
    1265                 1270                 1275             


Arg Ile  Phe Glu Leu Gly Ser  Lys Phe Pro Leu Gly  Gln Phe Gly 
    1280                 1285                 1290             


Asn Lys  Lys Phe Lys Tyr Val  Glu Thr Ala Lys Gly  Thr Asn Leu 
    1295                 1300                 1305             


Phe Phe  Ala Val Tyr Glu Asp  Glu Asn Lys Asn Arg  Asn Tyr Glu 
    1310                 1315                 1320             


Thr Ile  Pro Leu Asn Val Val  Ile Glu Arg Gln Lys  Gln Gly Leu 
    1325                 1330                 1335             


Ser Ser  Val Pro Ile Lys Ser  Glu Lys Gly His Lys  Leu Leu Phe 
    1340                 1345                 1350             


Tyr Leu  Ser Pro Asn Asp Ile  Val Tyr Val Pro Gly  Ser Asn Glu 
    1355                 1360                 1365             


Glu Phe  Ser Ile Asp Arg Leu  Tyr Arg Phe Thr Asp  Ser Ser Asp 
    1370                 1375                 1380             


Lys Thr  Ala Asn Phe Ile Pro  Leu Ser Val Ser Ser  Leu Ile Phe 
    1385                 1390                 1395             


Ser Ser  Asn Lys Asn Glu Gln  Lys Lys Ile Gly Ile  Ser Tyr Pro 
    1400                 1405                 1410             


Ile Gln  Asp Glu Phe Gly Leu  Gly Ser Pro Gln Ser  Lys Asn Gln 
    1415                 1420                 1425             


Lys Ser  Ile Asp Gly Ile Met  Ile Lys Glu Lys Cys  Val Lys Val 
    1430                 1435                 1440             


Asn Ile  Asp Arg Leu Gly Arg  Val Ser Phe 
    1445                 1450             


<210>  1269
<211>  1455
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1269

Met Ala Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Glu Asp Gln Asn Asn Lys Thr Thr Leu Ile Asp Lys Gly 
            20                  25                  30          


Val Arg Ile Phe Ser Glu Gly Val Lys Ile Glu Lys Gly Ile Glu Lys 
        35                  40                  45              


Ser Lys Ala Ala Glu Arg Thr Asp Phe Arg Ser Ala Arg Lys Ile Lys 
    50                  55                  60                  


Phe Arg Arg Arg Leu Arg Lys Gln Glu Thr Leu Lys Val Leu Ile Glu 
65                  70                  75                  80  


Asn Asn Met Cys Pro Leu Thr Ile Glu Glu Trp Thr Lys Trp Lys Lys 
                85                  90                  95      


Asp Lys Lys Glu Tyr Pro Lys Asn Pro Asp Phe Ile Asn Trp Leu Lys 
            100                 105                 110         


Thr Asp Glu Leu Lys Asn Ile Asn Pro Tyr Phe Phe Arg Asp Lys Ala 
        115                 120                 125             


Ser Arg Thr Glu Leu Pro Leu Met Gln Thr Gly Arg Ala Leu Tyr His 
    130                 135                 140                 


Leu Ala Gln Arg Arg Gly Phe Leu Ser Asn Arg Leu Asp Gln Gly Ser 
145                 150                 155                 160 


Asp Gly Phe Thr Glu Gln Ile Arg Leu Asp Leu Ile Lys Lys Ile Glu 
                165                 170                 175     


Cys Glu Thr Asp Lys Thr Glu Leu Leu Thr Ser Val Gln Ser Ile Ile 
            180                 185                 190         


Asp Thr Tyr Ser Glu Glu Glu Asp Lys Asp Ala Lys Lys Leu Leu Lys 
        195                 200                 205             


Gly Val Glu Lys Leu Cys Lys Ile Asn Asn Glu Leu Pro Ile Thr Glu 
    210                 215                 220                 


Phe Lys Glu Lys Leu Ile Asn Tyr Leu Asn Lys Ala Asp Asn Leu Gly 
225                 230                 235                 240 


Ala Val Lys Lys Gly Ile Ala Asp Val Ser Lys Lys Ile Thr Glu Thr 
                245                 250                 255     


Gly Cys Glu Thr Leu Gly Gln Tyr Phe Tyr Lys Leu Tyr Gln Asn Gln 
            260                 265                 270         


Ser Lys Ile Arg Lys Val Tyr Thr Ala Arg Thr Glu His Tyr Tyr His 
        275                 280                 285             


Glu Phe Leu Lys Ile Cys Glu Thr Gln Asn Ile Cys Asp Gln Leu Lys 
    290                 295                 300                 


His Lys Leu Asp Lys Ala Ile Phe Tyr Gln Arg Pro Leu Lys Ser Gln 
305                 310                 315                 320 


Lys Gly Leu Ile Gly Lys Cys Val Phe Glu Lys Asn Lys Thr Arg Cys 
                325                 330                 335     


Ala Val Ser His Pro Ala Tyr Glu Glu Tyr Arg Ala Leu Gln Phe Ile 
            340                 345                 350         


Asn Ser Ile Lys Val Ser Glu Thr Lys Asp Gly Lys Lys Asp Phe Leu 
        355                 360                 365             


Ser Phe Asp Glu Arg Lys Lys Ile Trp Pro Lys Phe Ile Arg Lys Ser 
    370                 375                 380                 


Lys Pro Asn Phe Asp Phe Lys Glu Ile Ala Glu Thr Leu Asp Lys Lys 
385                 390                 395                 400 


Gly Leu Ser Arg Tyr Tyr Asn Tyr Thr Gly His Thr Ser Val Ser Gly 
                405                 410                 415     


Cys Pro Thr Ile Ala Gln Leu Thr Asn Ile Phe Gly Glu Asn Trp Gln 
            420                 425                 430         


Glu Thr Leu Phe Glu Lys Tyr Thr Asn Lys Gln Asn Thr Lys Gly Leu 
        435                 440                 445             


Lys Thr Ile Asp Glu Val Val Thr Asp Ile Trp His Val Leu Phe Thr 
    450                 455                 460                 


Phe Asp Lys Asp Glu Lys Ile Ile Glu Phe Gly Lys Gln Lys Leu Gly 
465                 470                 475                 480 


Leu Asn Glu Val Leu Ala Lys Lys Phe Gly Ala Ile Asn Leu Lys Lys 
                485                 490                 495     


Asp Tyr Ala Ser Leu Ser Leu Lys Ala Ile Asn Lys Ile Leu Pro Tyr 
            500                 505                 510         


Leu Arg Glu Gly Leu Ile Tyr Ser Tyr Ala Val Phe Leu Ala Asn Ile 
        515                 520                 525             


Glu Asn Val Val Gly Glu Glu Ile Trp Lys Asn Asp Glu Asn Lys Asn 
    530                 535                 540                 


Leu Ile Lys Lys Glu Leu His Leu Leu Ile Asp Asn Phe Asn Glu Ile 
545                 550                 555                 560 


Lys Gly Leu Glu Ala Val Val Asn Asp Phe Ile Ala Asp Phe Lys Lys 
                565                 570                 575     


Glu Tyr Asn Asn Ala His Lys Asp Tyr Val Leu Asp Glu Gln Asp Lys 
            580                 585                 590         


Lys Ile Leu Leu Glu Lys Ile Glu Ser Asn Tyr Gly Lys Asn Lys Phe 
        595                 600                 605             


Glu Ser Leu Glu Pro Asp Lys Lys Thr Glu Ile Ile Asn Ser Thr Glu 
    610                 615                 620                 


Lys Met Val Ala Glu Gln Leu Arg Lys Gln Arg Gly Glu Phe Ile Lys 
625                 630                 635                 640 


Pro Lys Arg Leu Asp Glu Lys Ile Ser Glu Phe Leu Ile Asn Asn Phe 
                645                 650                 655     


Asn Val Thr Lys Lys Ser Ile Ala Arg Leu Tyr His Pro Ser Asp Val 
            660                 665                 670         


Glu Ile Phe Lys Thr Ala Ser Arg Asn Ser Glu Gly Leu Leu Leu Leu 
        675                 680                 685             


Gly Ser Pro Ile Val Ser Ser Ile Lys Asn Pro Met Ala Met Arg Ala 
    690                 695                 700                 


Met His Gln Leu Arg Lys Leu Val Asn Thr Leu Ile Lys Glu Asn Ile 
705                 710                 715                 720 


Ile Asp Glu Glu Thr Lys Ile Asn Ile Glu Leu Ala Arg Glu Leu Asn 
                725                 730                 735     


Asp Ala Asn Lys Arg Ala Ala Ile Lys Lys Trp Gln Asp Glu Arg Gln 
            740                 745                 750         


Lys Ala Arg Glu Glu Tyr Lys Lys Ser Ile Lys Glu Leu Phe Lys Ala 
        755                 760                 765             


Glu Cys Gly Lys Asp Ile Glu Pro Ser Asp Ser Glu Ile Leu Lys Tyr 
    770                 775                 780                 


Glu Leu Trp Glu Glu Gln Gln His Ile Cys Ile Tyr Thr Gly Lys Thr 
785                 790                 795                 800 


Ile Asn Ile Cys Asp Phe Ile Gly Ala Asn Pro Asn Phe Asp Ile Glu 
                805                 810                 815     


His Thr Ile Pro Lys Ser Leu Cys Phe Asp Asn Ser Asn Glu Asn Leu 
            820                 825                 830         


Thr Leu Cys Asp Ile Gln Tyr Asn Arg Asp Val Lys Arg Asn Lys Ile 
        835                 840                 845             


Pro Thr Glu Leu Ser Asp Tyr Asn Ser Ile Lys Pro Arg Leu Asp Lys 
    850                 855                 860                 


Trp Leu Val Phe Ile Asp Asp Tyr Glu Lys Leu Tyr Asn Thr Arg Lys 
865                 870                 875                 880 


Lys Ala Lys Gly Ile Glu Thr Lys Glu Gln Lys Asp Lys Arg Ile Lys 
                885                 890                 895     


Glu Lys His Tyr Tyr Lys Leu His Leu Asp Tyr Trp Lys Gly Lys Tyr 
            900                 905                 910         


Lys Arg Phe Ile Met Val Asp Val Pro Ser Gly Phe Lys Asn Ser Gln 
        915                 920                 925             


Ile Val Asp Thr Gly Ile Ile Thr Lys Tyr Ala Ile Ser Tyr Met Lys 
    930                 935                 940                 


Ser Ile Phe Pro Lys Val Tyr Ser Val Lys Gly Asn Ile Val Ala Glu 
945                 950                 955                 960 


Phe Arg Lys Ile Trp Gly Leu Gln Asn Asp Tyr Glu Lys Lys Glu Arg 
                965                 970                 975     


Val Asn His Val His His Cys Ile Asp Ala Ile Thr Ile Ala Cys Met 
            980                 985                 990         


Ser Lys Gln Lys Tyr Asp Thr Leu  Ala Lys Ile Tyr His  Glu Asp Glu 
        995                 1000                 1005             


Ala Gly  Phe His Lys Ile Lys  Lys Glu Thr Gln Leu  Val Ser Lys 
    1010                 1015                 1020             


Pro Trp  Thr Ser Phe Thr Met  Asp Val Leu Asn Ile  Glu Lys Glu 
    1025                 1030                 1035             


Ile Phe  Val Ser His His Thr  Pro Asp Asn Met Lys  Lys Gln Ser 
    1040                 1045                 1050             


Lys Lys  Val Val Arg Glu Arg  Gly Ile Ile Ala Pro  Lys Val Ile 
    1055                 1060                 1065             


Tyr Glu  Thr Asp Ala Asn Asn  Asn Thr Leu Arg Asp  Lys Tyr Gly 
    1070                 1075                 1080             


Lys Lys  Ile Val Lys Glu Tyr  Ile Leu Leu Lys Asp  Val Asn Gly 
    1085                 1090                 1095             


Asn Leu  Ile Pro Ile Lys Gly  Lys Arg Leu Thr Pro  Ser Asp Ile 
    1100                 1105                 1110             


Ile Asn  Lys Val Asp Gly Ile  Asp Tyr Phe Ser Leu  Ile Ile Asn 
    1115                 1120                 1125             


Asp Lys  Ile Gln Tyr Tyr Glu  Phe Ala Lys Ser Lys  Lys Thr Gly 
    1130                 1135                 1140             


Lys Ile  Ile Tyr Lys Lys Glu  Pro Ile Tyr Leu Gln  Gly Asp Thr 
    1145                 1150                 1155             


Val Arg  Gly Ser Leu His Leu  Asp Thr Phe Tyr Gly  Ala Ile Ala 
    1160                 1165                 1170             


Lys Asp  Ile Asn Gly Asn Ile  Ile Thr Asn Thr Asn  Gly Lys Ile 
    1175                 1180                 1185             


Ala Pro  Asn Tyr Val Ile Arg  Lys Glu Leu Ser Lys  Leu Lys Glu 
    1190                 1195                 1200             


Thr Asp  Ile Glu Asn Ile Val  Asp Pro Val Val Arg  Gly Lys Ile 
    1205                 1210                 1215             


Ile Glu  Ala Lys Lys Asn Lys  Leu Ile Thr Phe Asn  Lys Ser Gly 
    1220                 1225                 1230             


Ala Val  Ile Lys Thr Thr Ile  Trp Met Lys Glu Asn  Gln Ile Pro 
    1235                 1240                 1245             


Ile Asn  Lys Val Arg Val Tyr  Ser Ser Leu Lys Asn  Pro Leu Pro 
    1250                 1255                 1260             


Asp Phe  Lys Lys His Arg Asp  Leu Ser His Leu Thr  Tyr Lys Gln 
    1265                 1270                 1275             


Gln Tyr  Asn Val Ser Asn Glu  Glu Asn Tyr Cys Leu  Ala Ile Tyr 
    1280                 1285                 1290             


Glu Gly  Lys Asn Lys Lys Gly  Asn Val Cys Arg Thr  Cys Glu Leu 
    1295                 1300                 1305             


Val Asn  Met Ile Asp Ala Gly  Asn Tyr Tyr Arg Leu  Ser Asn Lys 
    1310                 1315                 1320             


Asn Trp  Lys Gln Gln Tyr Pro  Ile Val Pro Glu Lys  Asp Ser Val 
    1325                 1330                 1335             


Thr Asn  Tyr Ser Leu Lys Thr  Ile Ile Thr Lys Gly  Leu Met Val 
    1340                 1345                 1350             


Leu Leu  Tyr Glu Lys Ser Pro  Glu Glu Ile Trp Glu  Leu Thr Tyr 
    1355                 1360                 1365             


Asn Gln  Gln Met Glu Arg Leu  Tyr Glu Ile Thr Gln  Leu Asp Thr 
    1370                 1375                 1380             


Glu Lys  Ser Ala Glu Ile Lys  Leu Leu Phe His Gln  Glu Ala Arg 
    1385                 1390                 1395             


Glu Lys  Lys Val Ile Thr Glu  Tyr Met Gly Leu Lys  Thr Gly Met 
    1400                 1405                 1410             


Lys Gly  Gly Lys Asn Ile Gly  Lys His Lys Glu Phe  Pro Trp Ile 
    1415                 1420                 1425             


Lys Val  Gly Pro Asn Ser Phe  Asp Ala Leu Ile Glu  Gly Ile Asp 
    1430                 1435                 1440             


Phe Lys  Leu Thr Pro Thr Gly  Lys Ile Ile Lys Leu  
    1445                 1450                 1455 


<210>  1270
<211>  1457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1270

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Val Ser Val Asp Asp Tyr Gly Asn Tyr Leu His Asp Ile Lys 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly Asn Phe 
        35                  40                  45              


Asp Ser Gly Val Thr Val Ser Gln Thr Ala Gln Arg Thr Glu Phe Arg 
    50                  55                  60                  


Ser Lys Arg Arg Leu Val Gln Arg Asn Leu Gln Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Val Leu Asp Phe Leu Pro Ala His Tyr Asp Ala 
                85                  90                  95      


Ala Ile Gly Trp Asp Arg Thr Glu Ala Val Thr Tyr Gly Lys Phe Leu 
            100                 105                 110         


Asn His Ala Glu Pro Lys Leu Ala Trp Glu Glu Leu Gly Asn Gly Lys 
        115                 120                 125             


Arg Arg Phe Leu Phe Met Asp Ser Phe His Glu Met Val Ala Asp Phe 
    130                 135                 140                 


Ala Arg Cys Gln Pro Ala Leu Val Ala Asp Gly Lys Lys Ile Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Ala Ile 
                165                 170                 175     


Thr Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu Asn Pro Thr Lys 
        195                 200                 205             


Gln Glu Glu Tyr Tyr Glu Leu Lys Val Val Ser Val Glu Ala Thr Asp 
    210                 215                 220                 


Ser Arg Lys Gly Asn Asp Thr Trp Tyr Asn Val His Leu Glu Asn Gly 
225                 230                 235                 240 


Trp Ile Tyr Arg Arg Ser Ser Lys Ile Ser Leu Asp Asp Trp Val Gly 
                245                 250                 255     


Lys Val Lys Gln Phe Ile Val Thr Thr Glu Tyr Glu Lys Asp Gly Ala 
            260                 265                 270         


Thr Leu Lys Thr Asp Lys Glu Gly Tyr Val Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Pro Asn Glu Asn Asp Trp Gly Leu Arg Lys Lys Arg Thr Glu Thr Leu 
    290                 295                 300                 


Leu Glu Met Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Asp His Leu 
305                 310                 315                 320 


Leu Gln Glu Pro Ala Asp Lys Ile Arg Gly Asn Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg His Tyr Tyr Lys Ala Glu Leu Glu Ala Ile Leu Ala Glu Gln 
            340                 345                 350         


Cys Lys Tyr His Ile Glu Leu Arg Asp Lys Glu Met Leu Glu Ala Cys 
        355                 360                 365             


Ala Arg Glu Leu Tyr Leu Asn Asn Leu Pro His Gln Arg Ser Leu Met 
    370                 375                 380                 


Lys Lys Asp Met Thr Tyr Leu Leu Leu Tyr Asp Leu Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ala Leu Ile Ala Asn Cys Pro Tyr Glu 
                405                 410                 415     


Tyr Tyr Glu Tyr Val Asp Lys Glu Thr Gly Glu Ile Lys Arg Gln Asn 
            420                 425                 430         


Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Phe Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Val Leu Asn Leu Arg Leu Phe Asp Arg Val Glu Asp Lys 
    450                 455                 460                 


Glu Val Thr Ala Asn Tyr Leu Pro Thr Ala Asp Asp His Ala Arg Leu 
465                 470                 475                 480 


Phe Thr Tyr Leu Asn Asp Arg Lys Glu Ile Asn Gln Glu Thr Leu Leu 
                485                 490                 495     


Lys Asp Tyr Phe Lys Leu Lys Lys Val Lys Ile Gly Asn Glu Lys Glu 
            500                 505                 510         


Tyr Pro Ile Arg Trp Asn Tyr Ile Glu Asp Lys Glu Lys Lys Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg Tyr Glu Leu Leu Met Ala Leu Asp Arg Ala Gly 
    530                 535                 540                 


Ile Glu His Lys Trp Leu Asp Asp Asn Asn Leu Glu Tyr Arg Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Val Glu Val Lys Ser Glu Ser Glu Thr Ala Leu 
                565                 570                 575     


Arg Lys Leu Lys Asn Asp Asp Ala Phe Val Asn Ser Phe Leu Lys Val 
            580                 585                 590         


Lys Pro Phe Lys Lys Glu Tyr Gly Ala Tyr Ser Glu Lys Ala Ile Lys 
        595                 600                 605             


Arg Leu Leu Ala Val Met Arg Lys Gly Phe Leu Trp Asn Glu Gln Asp 
    610                 615                 620                 


Ile Cys Asp Glu Thr Arg Lys Arg Ile Glu Ile Ile Ile Lys Gly Asp 
625                 630                 635                 640 


Val Asp Glu Lys Val Lys Glu Arg Met Gly His Ala Ala Tyr Ser Phe 
                645                 650                 655     


Asp Arg Val Ser Asp Phe Gln Gly Leu Pro Glu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Val Val Tyr Gly Arg His Ser Glu Ala Met Asp Ile Gln His Trp Asp 
        675                 680                 685             


Ser Pro Glu Arg Leu Met Ala Tyr Ile Asn Asn Phe Lys Gln Tyr Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Cys Ile Leu Glu Thr Leu Arg Thr 
705                 710                 715                 720 


Val His Asp Ile Trp Lys Glu Thr Gly Gln Ile Asp Glu Ile His Val 
                725                 730                 735     


Glu Leu Gly Arg Glu Met Lys Ser Thr Ser Glu Glu Arg Ala Arg Arg 
            740                 745                 750         


Thr Gln Asn Val Leu Arg Asn Glu Asn Thr Asn Leu Arg Ile Lys Ser 
        755                 760                 765             


Leu Leu Ile Glu Leu Lys Asn Asp Thr Asp Ile Cys Asn Val His Pro 
    770                 775                 780                 


Tyr Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala 
785                 790                 795                 800 


Leu Gln Glu Leu Asn Arg Glu Asp Glu Asp Phe Glu Glu Ile Ser Lys 
                805                 810                 815     


Ile Ser Gln Met Ser Gln Pro Ser Gly Lys Glu Leu Asn Arg Tyr Lys 
            820                 825                 830         


Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Ala Ile 
        835                 840                 845             


Ser Leu Ala Lys Leu Phe Thr Pro Ala Tyr Gln Ile Glu His Val Ile 
    850                 855                 860                 


Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Thr Asn Lys Val Ile Cys 
865                 870                 875                 880 


Glu Ala Glu Val Asn Thr Leu Lys Ser Asn Leu Leu Gly Tyr Glu Phe 
                885                 890                 895     


Ile Lys Lys His Gly Gly Glu Ile Ile His Cys Thr Thr Leu Gly Asp 
            900                 905                 910         


Val Arg Ile Phe Ser Glu Ser Glu Tyr Lys Ala Phe Val Met Asp His 
        915                 920                 925             


Tyr Ala Asn Asn Lys Ser Lys Lys Asp Lys Leu Leu Met Glu Asp Ile 
    930                 935                 940                 


Pro Gln Glu Phe Leu Ser Arg Gln Met Asn Asp Ser Arg Tyr Ile Ser 
945                 950                 955                 960 


Lys Val Val Lys Thr Leu Leu Ser Asn Ile Val Arg Met Ala Asp Glu 
                965                 970                 975     


Ser Glu Ala Thr Ser Lys Ser Val Ile Val Cys Thr Gly Gly Val Thr 
            980                 985                 990         


Asp Arg Leu Lys Lys Asp Trp Gly  Leu Asn Asp Val Trp  Asn Ser Ile 
        995                 1000                 1005             


Val Tyr  Ser Arg Phe Glu Arg  Leu Asn Arg Leu Thr  Gly Thr Glu 
    1010                 1015                 1020             


Ala Phe  Gly His Trp Glu Asn  Lys Asp Gly Lys Arg  Val Phe Gln 
    1025                 1030                 1035             


Thr Ser  Met Pro Leu Glu Leu  Gln Arg Gly Phe Arg  Lys Lys Arg 
    1040                 1045                 1050             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1055                 1060                 1065             


Ala Ser  Arg Asn Ile Val Asn  Tyr Leu Asn Asn Glu  Ser Ala Asn 
    1070                 1075                 1080             


Asn Pro  Lys Lys Arg Glu Asp  Leu Arg Arg Gln Leu  Cys Asp Lys 
    1085                 1090                 1095             


Asn Arg  Val Ile Arg Lys Pro  Trp Glu Thr Phe Thr  Gln Asp Ala 
    1100                 1105                 1110             


Tyr Asn  Ala Leu Glu Asn Val  Val Val Ser Phe Lys  Asn Tyr Val 
    1115                 1120                 1125             


Arg Val  Ile Asn Lys Ala Thr  Asn Arg Tyr Glu His  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Gly  Lys Lys Arg Phe Val  Gln Gln Lys Gly Ser  Glu Leu Trp 
    1145                 1150                 1155             


Ala Ile  Arg Lys Pro Met His  Lys Glu Thr Tyr Tyr  Gly His Val 
    1160                 1165                 1170             


Asn Leu  Arg Arg Lys Glu Leu  Val Thr Leu Gln Lys  Ala Leu Asp 
    1175                 1180                 1185             


Asp Ile  Pro Ser Ile Cys Asp  Lys Lys Leu Arg Asp  Tyr Ile Ser 
    1190                 1195                 1200             


Ala Leu  Val Glu Lys His Phe  Asn Lys Arg Gln Leu  Leu Ala His 
    1205                 1210                 1215             


Phe Lys  Ser Thr Asp Tyr Tyr  Trp Gln Lys Gln Tyr  Val Asn Lys 
    1220                 1225                 1230             


Val Tyr  Val Trp Thr Phe Ser  Asp Asp Ile Lys Pro  Leu Val Ala 
    1235                 1240                 1245             


Thr Arg  Lys Leu Leu Asp Thr  Ser Phe Asp Ala Lys  Arg Ile Gly 
    1250                 1255                 1260             


Thr Ile  Thr Asp Thr Gly Ile  Gln Lys Ile Leu Arg  Asn Tyr Leu 
    1265                 1270                 1275             


Glu Ala  Lys Gly Gly Asn Ala  Thr Leu Ala Phe Thr  Pro Glu Gly 
    1280                 1285                 1290             


Ile Ala  Glu Met Asn Gln Asn  Ile Ser Lys Tyr Asn  Asp Gly Lys 
    1295                 1300                 1305             


Pro His  Gln Pro Ile Leu Lys  Val Arg Ile Thr Glu  Pro Met Gly 
    1310                 1315                 1320             


Ala Lys  Tyr Arg Val Gly Gln  Thr Gly Asn Lys Met  Ala Lys Tyr 
    1325                 1330                 1335             


Val Glu  Ala Gln Ser Gly Thr  Asn Leu Tyr Phe Ala  Ile Tyr Glu 
    1340                 1345                 1350             


Asp Ser  Glu Gly Lys Arg Ser  Tyr Thr Thr Val Pro  Leu Asp Glu 
    1355                 1360                 1365             


Val Ala  Glu Arg Leu Lys Gln  Arg Leu Ser Pro Val  Pro Glu Lys 
    1370                 1375                 1380             


Asp Glu  Asn Asp Val Pro Leu  Lys Phe Tyr Leu Ser  Pro Asn Asp 
    1385                 1390                 1395             


Leu Val  Tyr Val Pro Thr Glu  Glu Glu Leu Gln Leu  Arg Glu Cys 
    1400                 1405                 1410             


Lys Leu  Asn Lys Asp Arg Ile  Tyr Lys Met Val Ser  Ser Thr Gly 
    1415                 1420                 1425             


Asn Glu  Cys His Phe Ile Pro  Tyr Tyr Val Ala Asn  Val Ile Tyr 
    1430                 1435                 1440             


Asn Lys  Gly Gly Phe Asn Met  Gln Met His Arg Pro  Gln Ser 
    1445                 1450                 1455         


<210>  1271
<211>  1457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1271

Met Ala Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Ile Val Glu Lys Asn Asn Ser Asp Phe Thr Leu Ile Asp Lys Gly 
            20                  25                  30          


Val Arg Ile Phe Ser Glu Gly Val Lys Thr Glu Lys Gly Ile Glu Arg 
        35                  40                  45              


Ser Arg Ala Ala Glu Arg Thr Gly Tyr Arg Ser Ala Arg Lys Ile Lys 
    50                  55                  60                  


Tyr Arg Arg Lys Leu Arg Lys Tyr Glu Thr Leu Lys Val Leu Ser Lys 
65                  70                  75                  80  


Asn Arg Met Cys Pro Leu Gln Ile Glu Asp Val Glu Ala Trp Lys Lys 
                85                  90                  95      


Ser Gly Phe Lys Asp Tyr Pro Leu Ile Pro Glu Phe Leu Gln Trp Leu 
            100                 105                 110         


Arg Thr Asn Asp His Glu Asn Val Asn Pro Tyr Phe Phe Arg Asp Lys 
        115                 120                 125             


Ala Ser Arg Ala Lys Val Ser Leu Trp Glu Leu Gly Arg Ala Phe Tyr 
    130                 135                 140                 


His Ile Ala Gln Arg Arg Gly Phe Leu Ser Asn Arg Leu Asp Gln Ser 
145                 150                 155                 160 


Ser Glu Gly Val Leu Glu Glu His Asn Pro Gln Ile Gln Ser Leu Ile 
                165                 170                 175     


Glu Asp Leu Glu Glu Thr Ser Thr Ile Leu Asn Glu Val Arg Asp Tyr 
            180                 185                 190         


Tyr Ile Asn Leu Gly Leu Leu Asp Gly Val Lys Gly Asp Phe Lys Lys 
        195                 200                 205             


Glu Leu Asp Glu Gly Glu Lys Lys Leu Lys Ser Leu Tyr Asn Thr Leu 
    210                 215                 220                 


Ile Ala Ile Ile Lys Lys Asn Glu Asn Asp Val Gln Lys Cys Lys Asp 
225                 230                 235                 240 


Glu Ile Ile Lys Arg Leu Asn Lys Lys Glu Asp Leu Gly Lys Val Lys 
                245                 250                 255     


Gly Lys Ile Lys Asp Ile Ser Gln Ala Met Leu Asp Gly Asn Phe Lys 
            260                 265                 270         


Thr Leu Gly Gln Tyr Phe Tyr Ser Leu Tyr Asn Lys Gly Lys Ile Arg 
        275                 280                 285             


Asn Gln Tyr Thr Ser Arg Glu Glu His Tyr Leu Gln Glu Phe Ile Thr 
    290                 295                 300                 


Ile Cys Lys Val Gln Gly Ile Asn Gly Ile Asn Asp Glu Glu Ile Leu 
305                 310                 315                 320 


Pro Glu Lys Lys Phe Thr Gly Leu Ala Lys Asp Leu Tyr Lys Ala Ile 
                325                 330                 335     


Phe Phe Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Ile Gly Lys Cys 
            340                 345                 350         


Ser Phe Glu Lys Asn Lys Ser Arg Cys Ala Ile Ser His Pro Asp Phe 
        355                 360                 365             


Glu Glu Tyr Arg Met Trp Asn Tyr Leu Asn Thr Ile Lys Phe Gly Thr 
    370                 375                 380                 


Gln Ser Asp Lys Lys Leu Arg Phe Leu Thr Gln Glu Glu Lys Leu Lys 
385                 390                 395                 400 


Leu Ile Pro Lys Phe Tyr Arg Lys Asn Asp Phe Asn Phe Glu Val Leu 
                405                 410                 415     


Ala Lys Glu Leu Ile Asp Lys Gly Ala Thr Phe Gly Phe Tyr Lys Ser 
            420                 425                 430         


Ser Arg Lys Asn Glu Phe Leu Tyr Trp Phe Asn Tyr Lys Pro Thr Asp 
        435                 440                 445             


Thr Val Ser Ala Cys Gln Phe Ser Ala Ser Leu Lys Asn Ala Ile Gly 
    450                 455                 460                 


Glu Asp Trp Gln Thr Lys Val Phe Thr Tyr Lys Thr Leu Asn Ser Glu 
465                 470                 475                 480 


Lys Lys Glu Val Ile Arg Thr Val Asp Tyr Arg Asp Leu Trp His Leu 
                485                 490                 495     


Leu Ser Ala Ser Thr Ser Asp Ile Tyr Leu Tyr Glu Tyr Ala Lys Glu 
            500                 505                 510         


Lys Leu Gly Leu Glu Asp Lys Ser Ala Lys Thr Phe Ser Lys Ile Lys 
        515                 520                 525             


Leu Lys Lys Asp Phe Ala Ser Leu Ser Leu Ser Ala Ile Asn Lys Ile 
    530                 535                 540                 


Leu Pro Tyr Ile Lys Glu Gly Leu Leu Tyr Ser His Ala Val Phe Met 
545                 550                 555                 560 


Ala Asn Ile Glu Lys Val Val Asp Glu His Ile Trp Lys Asp Val Lys 
                565                 570                 575     


Glu Arg Thr Ile Ile Gln Asn Glu Ile Ser Lys Ile Ile Lys Ser Asp 
            580                 585                 590         


Val Phe Glu Arg Glu Val Ile Asn Ile Ile Asn Gly Leu Ile Lys Asn 
        595                 600                 605             


Ser Lys Thr Glu Gly Glu Asn Tyr Ala Glu Glu Ser Lys Asn Ile Phe 
    610                 615                 620                 


Lys Thr Glu Phe Asp Asn Ala Ile Ala Lys Ile Phe Glu Lys Asn Asn 
625                 630                 635                 640 


Asp Ile Glu Phe Asn Gln Pro Phe Leu Asp Asp Thr Tyr Leu Lys Tyr 
                645                 650                 655     


Val Glu Glu Leu Lys Lys Phe Asp Lys Ala Arg Ser Phe Leu Pro Val 
            660                 665                 670         


Pro Arg Leu Asp Glu Lys Val Ile Gln Phe Leu Leu Gly Asn Asn Lys 
        675                 680                 685             


Asp Gly Met Val Tyr Cys Ser Asp Lys Asn Lys Thr Lys Lys Leu Tyr 
    690                 695                 700                 


His Pro Ser Asp Ile Glu Ile Phe Lys Lys Lys Ile Ile Lys Asp Glu 
705                 710                 715                 720 


Phe Gly Asn Glu Lys Met Val Leu Gly Ser Pro Leu Thr Thr Ser Ile 
                725                 730                 735     


Lys Asn Pro Met Ala Met Arg Ala Leu His Gln Leu Arg Lys Val Leu 
            740                 745                 750         


Asn Thr Leu Ile Leu Glu Glu Gln Ile Asp Glu Asn Thr Arg Ile His 
        755                 760                 765             


Ile Glu Met Ala Arg Glu Leu Asn Asp Ala Asn Lys Arg Lys Gly Ile 
    770                 775                 780                 


Gln Asp Tyr Gln Asn Glu Asn Lys Lys Phe Arg Glu Asp Ala Ile Lys 
785                 790                 795                 800 


Glu Ile Lys Lys Leu Tyr Phe Glu Glu Cys Lys Lys Glu Val Glu Pro 
                805                 810                 815     


Thr Glu Glu Asp Val Leu Arg Tyr Gln Leu Trp Ile Glu Gln Asp Lys 
            820                 825                 830         


Lys Glu Ile Tyr Glu Gln Gly Lys Ser Ile Arg Ile Ser Asp Ile Ile 
        835                 840                 845             


Gly Ser Asn Pro Ala Tyr Asp Ile Glu His Thr Val Pro Arg Ser Ile 
    850                 855                 860                 


Ser Gln Asn Asn Ser Leu Met Asn Lys Thr Leu Cys Thr Pro Arg Phe 
865                 870                 875                 880 


Asn Arg Glu Ile Lys Lys Gln Lys Met Pro Ile Glu Leu Ser Asn Tyr 
                885                 890                 895     


Glu Glu Ile Leu Pro Arg Ile Glu His Trp Lys Lys Glu Ala Glu Asp 
            900                 905                 910         


Leu Thr Arg Gln Ile Glu Ser Ile Ser Lys Ser Ile Lys Ser Ala Ser 
        915                 920                 925             


Thr Lys Glu Ala Lys Asp Lys Asn Ile Arg Arg Arg His Tyr Leu Thr 
    930                 935                 940                 


Leu Lys Arg Asp Tyr Leu Lys Gly Lys Tyr Asp Arg Phe Ile Trp Glu 
945                 950                 955                 960 


Glu Pro Lys Val Gly Phe Lys Asn Ser Gln Ile Pro Asp Thr Gly Ile 
                965                 970                 975     


Ile Thr Lys Tyr Ala Gln Ala Tyr Leu Lys Ser Tyr Phe Lys Arg Val 
            980                 985                 990         


Glu Ser Val Lys Gly Gly Met Val  Ala Glu Phe Arg Lys  Gln Trp Gly 
        995                 1000                 1005             


Ile Gln  Glu Ser Tyr Ile Asp  Glu Asn Gly Phe Lys  His Tyr Lys 
    1010                 1015                 1020             


Glu Lys  Asp Arg Ser Lys His  Thr His His Thr Ile  Asp Ala Ile 
    1025                 1030                 1035             


Thr Ile  Ala Cys Met Thr Lys  Asp Lys Tyr Asp Val  Leu Ala Gln 
    1040                 1045                 1050             


Ala Trp  Thr Ser Glu Asp Glu  Gln Asp Lys Gln Lys  Ala Lys Thr 
    1055                 1060                 1065             


Leu Met  Ser Glu Ala Lys Pro  Trp Lys Thr Phe Lys  Glu Asp Ile 
    1070                 1075                 1080             


Glu Lys  Ile Glu Glu Glu Ile  Leu Val Ser His Phe  Thr Leu Asp 
    1085                 1090                 1095             


Asn Val  Lys Lys Gln Ser Lys  Lys Ile Val Arg Val  Arg Gly Lys 
    1100                 1105                 1110             


Lys Gln  Tyr Val Ala Glu Ile  Glu Arg Asp Val Asn  Gly Lys Ala 
    1115                 1120                 1125             


Met Ala  Lys Lys Asp Ala Asn  Gly Lys Ile Ile Tyr  Lys Leu Asn 
    1130                 1135                 1140             


Glu Lys  Gly Glu Lys Ile Pro  Arg Leu Gln Gln Gly  Asp Thr Ile 
    1145                 1150                 1155             


Arg Gly  Ser Leu His Gln Asp  Ser Ile Tyr Gly Ala  Ile Lys Asn 
    1160                 1165                 1170             


Pro Leu  Asn Gln Glu Glu Ile  Lys Tyr Val Ile Arg  Lys Asp Leu 
    1175                 1180                 1185             


Glu Ser  Leu Lys Ala Asn Asp  Val Asp Asn Ile Val  Asp Glu Thr 
    1190                 1195                 1200             


Val Lys  Gln Lys Val Lys Glu  Ala Ile Glu Asn Lys  Ile Leu Leu 
    1205                 1210                 1215             


Leu Ser  Ser Asn Ala Gln Gln  Lys Asn Lys Leu Val  Asp Asp Val 
    1220                 1225                 1230             


Trp Leu  Asn Lys Glu Lys Gln  Val Pro Ile Lys Lys  Val Arg Ile 
    1235                 1240                 1245             


Tyr Ala  Asn Ser Val Lys Asn  Pro Leu Glu Ile Lys  Glu Gln Ser 
    1250                 1255                 1260             


Leu Leu  Ser Lys Ser Arg Lys  Glu His Lys Gln Lys  Val Tyr Gly 
    1265                 1270                 1275             


Gln Asn  Asp Glu Asn Tyr Ala  Met Ala Ile Tyr Glu  Leu Asp Gly 
    1280                 1285                 1290             


Lys Arg  Glu Phe Glu Leu Ile  Asn Asn Phe Asn Leu  Ala Lys Leu 
    1295                 1300                 1305             


Ile Lys  Gln Lys Glu Gly Phe  Tyr Pro Leu Asn Lys  Glu Lys Glu 
    1310                 1315                 1320             


Val Lys  Gly Lys Lys Val Leu  Tyr Pro Ile Ala Lys  Ser Asn Tyr 
    1325                 1330                 1335             


Arg Asp  Val Val Leu Lys Arg  Gly Gln Gln Val Val  Phe Tyr Asp 
    1340                 1345                 1350             


Lys Glu  Val Glu Asn Pro Lys  Asp Ile Ser Glu Val  Ile Asp Phe 
    1355                 1360                 1365             


Lys Gly  Arg Ile Tyr Ile Ile  Glu Gly Leu Ser Ile  Gln Arg Ile 
    1370                 1375                 1380             


Val Arg  Pro Ser Gly Lys Ile  Asp Glu Tyr Gly Val  Ile Met Leu 
    1385                 1390                 1395             


Lys Tyr  Phe Lys Glu Ala Arg  Lys Ala Asp Glu Ile  Lys Lys Asp 
    1400                 1405                 1410             


Asn Tyr  Lys Pro Asp Gly Val  Phe Lys Leu Gly Glu  Asn Arg Pro 
    1415                 1420                 1425             


Thr Arg  Lys Met Asn His Ser  Gln Phe Thr Ala Phe  Val Glu Gly 
    1430                 1435                 1440             


Ile Asp  Phe Lys Val Leu Pro  Thr Gly Lys Phe Asp  Lys Ile 
    1445                 1450                 1455         


<210>  1272
<211>  1458
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1272

Met Thr Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Ile Asn Gln Asp Phe Asp Asn Lys Lys Gly Glu Ile Leu 
            20                  25                  30          


Gly Met Gly Ser Arg Ile Ile Pro Met Thr Gln Asp Ile Lys Asp Glu 
        35                  40                  45              


Phe Gly Lys Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg Thr Arg Leu 
    50                  55                  60                  


Arg Gly Val Arg Arg Leu Ile His Arg Thr Leu Leu Arg Arg Glu Arg 
65                  70                  75                  80  


Leu His Arg Val Leu Asn Ile Ile Gly Phe Leu Pro Glu His Tyr Ala 
                85                  90                  95      


Asn Gln Ile Asp Phe Glu Lys Arg Phe Gly Lys Phe Lys Pro Glu Ser 
            100                 105                 110         


Glu Pro Lys Ile Ser Phe Asp Gly Asn Asp Val Phe Leu Phe Glu Lys 
        115                 120                 125             


Ser Tyr Gln Glu Met Leu Val Asp Phe Lys Ile His Gln Pro Gln Leu 
    130                 135                 140                 


Val Ser Asn Gly Lys Lys Ile Pro His Asp Trp Thr Ile Tyr Tyr Leu 
145                 150                 155                 160 


Arg Lys Lys Ala Leu Ser Lys Lys Ile Glu Lys Glu Glu Leu Ala Trp 
                165                 170                 175     


Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly 
            180                 185                 190         


Glu Glu Glu Glu Glu Thr Pro Asn Lys Leu Val Glu Phe His Ser Leu 
        195                 200                 205             


Lys Ile Val Glu Val Asn Ala Asp Glu Pro Gln Lys Gly Lys Pro Glu 
    210                 215                 220                 


Ile Trp Tyr Ser Leu Val Leu Glu Asn Gly Trp Val Tyr Arg Arg Ala 
225                 230                 235                 240 


Ser Lys Thr Pro Leu Phe Asp Trp Lys Asp Lys Ile Arg Glu Phe Ile 
                245                 250                 255     


Val Thr Thr Glu Ile Asn Asn Asp Gly Thr Val Lys Thr Asp Lys Glu 
            260                 265                 270         


Gly Thr Glu Lys Arg Ser Phe Arg Ala Pro Lys Pro Glu Asp Trp Thr 
        275                 280                 285             


Leu Gln Lys Lys Lys Thr Glu Phe Glu Leu Ser Lys Ser Gly Asn Glu 
    290                 295                 300                 


Val Gly Ala Phe Ile Tyr Glu Ser Ile Leu Gln Lys Pro Asn Gln Lys 
305                 310                 315                 320 


Ile Arg Gly Lys Leu Ile Ser Thr Ile Glu Arg Lys Phe Tyr Lys Glu 
                325                 330                 335     


Glu Leu Lys Thr Ile Leu Lys Thr Gln Leu Phe Phe His Lys Glu Leu 
            340                 345                 350         


Lys Asp Glu Lys Leu Tyr Asn Ala Cys Ile Glu Glu Leu Tyr Lys Asn 
        355                 360                 365             


Asn Glu Ala His Arg Ser Leu Leu Ser Asn Lys Gly Phe Glu His Leu 
    370                 375                 380                 


Phe Ile Asn Asp Ile Leu Phe Tyr Gln Arg Pro Leu Arg Ser Lys Lys 
385                 390                 395                 400 


Ser Gln Ile Ser Asn Cys Pro Leu Glu Lys Arg Thr Tyr Lys Lys Glu 
                405                 410                 415     


Gly Ile Glu Ile Thr Glu Gly Ile Lys Val Ile Ser Lys Ser Asn Pro 
            420                 425                 430         


Ile Tyr Gln Glu Phe Arg Leu Trp Gln Trp Ile Ser Asn Leu Ser Leu 
        435                 440                 445             


Tyr Cys Ile Glu Pro Thr Glu Thr Asn Val Thr Ser Thr Phe Leu Asn 
    450                 455                 460                 


Ser Ile Glu Asp Tyr Glu Asn Leu Phe Glu Phe Leu Asn Asn Arg Lys 
465                 470                 475                 480 


Glu Ile Glu Gln Lys His Leu Leu Lys Tyr Leu Leu Glu Asn Gln Gly 
                485                 490                 495     


Phe Lys Gly Lys Leu Leu Thr Asn Glu Leu Glu Lys Phe Arg Trp Asn 
            500                 505                 510         


Phe Val Ala Asp Lys Lys Tyr Pro Cys Asn Glu Thr Gly Ser Leu Leu 
        515                 520                 525             


His Thr Arg Leu Ser Lys Val Lys Asn Ile Ser Pro Asp Phe Leu Thr 
    530                 535                 540                 


Lys Glu Ile Glu His Gln Leu Trp His Ile Ile Tyr Ser Val Thr Asp 
545                 550                 555                 560 


Lys Ile Glu Tyr Glu Gln Ala Leu Lys Thr Phe Ala Arg Lys Asn Asn 
                565                 570                 575     


Leu Asp Val Asp Ser Phe Phe Glu His Phe Lys Lys Ile Pro Pro Phe 
            580                 585                 590         


Glu Ser Thr Tyr Gly Ala Tyr Ser Glu Lys Ala Ile Lys Lys Leu Leu 
        595                 600                 605             


Pro Leu Ile Arg Leu Gly Lys Tyr Trp Asn Trp Glu Ala Ile Asp Ser 
    610                 615                 620                 


Ile Ser Lys Asp Arg Ile Ser Lys Ile Leu Ser Gly Glu Tyr Asp Glu 
625                 630                 635                 640 


Asn Ile Lys Asn Arg Val Arg Glu Lys Ala Val His Leu Thr Ser Glu 
                645                 650                 655     


Asn Asn Phe Gln Gly Leu Gln Glu Trp Leu Ala Lys Tyr Ile Val Tyr 
            660                 665                 670         


Asp Arg His Ser Glu Gly Asn Asp Leu Gly Lys Trp Thr Ser Val Ser 
        675                 680                 685             


Asp Leu Glu Thr Tyr Leu Lys Glu Phe Lys Gln His Ser Leu Arg Asn 
    690                 695                 700                 


Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val Val Lys Asp 
705                 710                 715                 720 


Ile Trp Ile Lys His Gly Lys Gly Thr Glu Asn Phe Phe Asp Glu Ile 
                725                 730                 735     


His Val Glu Leu Gly Arg Glu Met Lys Asn Asn Ser Glu Asp Arg Lys 
            740                 745                 750         


Arg Leu Thr Asn Thr Ile Thr Glu Asn Glu Asn Thr Asn Leu Arg Ile 
        755                 760                 765             


Lys Ala Leu Leu Met Glu Met Met Asn Asp Asn Asp Val Glu Asn Val 
    770                 775                 780                 


Arg Pro Tyr Ser Pro Ser Gln Gln Glu Ile Leu Lys Ile Tyr Glu Asp 
785                 790                 795                 800 


Gly Ala Leu Asn Ser Asn Ile Glu Leu Asp Asp Glu Ile Val Lys Ile 
                805                 810                 815     


Ser Lys Lys Ala Glu Pro Thr Lys Ser Glu Leu Gln Arg Tyr Lys Leu 
            820                 825                 830         


Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Gln Val Ile Pro 
        835                 840                 845             


Leu Asn Lys Leu Phe Thr Ser Glu Tyr Glu Ile Glu His Val Val Pro 
    850                 855                 860                 


Gln Ser Arg Phe Phe Asp Asp Ser Phe Ser Asn Lys Val Ile Cys Glu 
865                 870                 875                 880 


Ser Ala Val Asn Lys Arg Lys Asp Asn Gln Leu Gly Leu Gln Phe Ile 
                885                 890                 895     


Lys Asn His Ser Gly Glu Lys Val Glu Leu Gly Phe Gly Lys Val Val 
            900                 905                 910         


Gln Val Phe Thr Glu Glu Gln Tyr Leu Asp Phe Val Lys Glu His Tyr 
        915                 920                 925             


Ser Lys Asn Arg Ser Lys His Asn Lys Leu Leu Leu Glu Glu Ile Pro 
    930                 935                 940                 


Glu Lys Met Ile Glu Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser Lys 
945                 950                 955                 960 


Phe Val Ser Ser Ile Leu Ser Asn Ile Val Arg Ser Glu Lys Asp Asp 
                965                 970                 975     


Asp Gly Leu Asn Ser Lys Asn Ile Leu Pro Gly Asn Gly Lys Ile Thr 
            980                 985                 990         


Thr Glu Leu Lys Arg Asp Trp Gly  Leu Asn Asp Val Trp  Asn Asp Leu 
        995                 1000                 1005             


Ile Leu  Pro Arg Phe Glu Arg  Met Asn Thr Ile Thr  Asn Ser Asp 
    1010                 1015                 1020             


Leu Phe  Thr Thr Tyr Asn Asp  Lys Tyr Gln Lys His  Leu Pro Thr 
    1025                 1030                 1035             


Val Pro  Phe Glu Tyr Ser Lys  Gly Phe Gln Lys Lys  Arg Ile Asp 
    1040                 1045                 1050             


His Arg  His His Ala Met Asp  Ala Leu Val Ile Ala  Cys Ala Thr 
    1055                 1060                 1065             


Arg Asp  His Leu Asn Leu Met  Asn Asn Gln Ser Ala  Lys Ser Glu 
    1070                 1075                 1080             


Leu Lys  Arg Tyr Asp Leu Gln  Asn Lys Leu Arg Lys  Lys Glu Pro 
    1085                 1090                 1095             


Tyr Phe  Asn Gln Lys Glu Asn  Lys Gln Lys Glu Ala  Phe Lys Asp 
    1100                 1105                 1110             


Phe Ile  Lys Pro Trp Gly Thr  Phe Thr Glu Asp Ser  Lys Asn Ala 
    1115                 1120                 1125             


Leu Glu  Lys Ile Ile Ile Ser  Phe Lys Gln Asn Leu  Arg Val Ile 
    1130                 1135                 1140             


Asn Lys  Ala Thr Asn Ser Tyr  Glu Ser Tyr Lys Asp  Glu Asp Gly 
    1145                 1150                 1155             


Asn Leu  Arg Ile Gly Lys Asp  Gly Lys Pro Glu Lys  Gly Leu Ile 
    1160                 1165                 1170             


Lys Gln  Lys Gly Leu Asn Trp  Ala Ile Arg Lys Pro  Leu His Lys 
    1175                 1180                 1185             


Asp Thr  Val Ser Gly Gln Ile  Asn Leu Ser Arg Ile  Lys Leu Pro 
    1190                 1195                 1200             


Asn Gly  Lys Ile Leu Thr Ala  Thr Arg Lys Asn Leu  Asp Thr Ser 
    1205                 1210                 1215             


Phe Asp  Leu Lys Thr Ile Glu  Asn Ser Ile Thr Asp  Thr Gly Ile 
    1220                 1225                 1230             


Gln Lys  Ile Leu Lys Asn Tyr  Leu Leu Ser Lys Glu  Ser Pro Glu 
    1235                 1240                 1245             


Leu Ala  Phe Ser Pro Glu Gly  Leu Glu Glu Met Asn  Asn Glu Ile 
    1250                 1255                 1260             


Glu Lys  Tyr Asn Asn Gly Lys  Phe His His Pro Ile  Asn Lys Ala 
    1265                 1270                 1275             


Arg Ile  Tyr Glu Leu Gly Ser  Lys Phe Asn Val Gly  Gln Thr Gly 
    1280                 1285                 1290             


Asn Arg  Arg Asp Lys Phe Val  Glu Ala Ala Lys Gly  Thr Asn Leu 
    1295                 1300                 1305             


Phe Phe  Ala Ile Tyr Gln Asp  Glu Asn Lys Asn Arg  Ser Tyr Glu 
    1310                 1315                 1320             


Thr Ile  Pro Leu Asn Glu Val  Ile Glu His Gln Lys  Trp Arg Ala 
    1325                 1330                 1335             


Ser Leu  Pro Lys Glu Glu Gln  Glu Lys Ile Pro Leu  Val Pro Val 
    1340                 1345                 1350             


Asn Lys  Leu Lys Gly Thr Phe  Ile Phe Ser Leu Ser  Pro Asn Asp 
    1355                 1360                 1365             


Leu Val  Tyr Val Pro Ser Asn  Asp Glu Leu Glu Arg  Ser Ala Ser 
    1370                 1375                 1380             


Ile Asp  Phe Ser Lys Leu Lys  Lys Glu Gln Ile Asn  Arg Leu Tyr 
    1385                 1390                 1395             


Lys Met  Val Ser Ser Ser Gly  Ser Gln Cys Phe Phe  Val Lys Ser 
    1400                 1405                 1410             


Glu Val  Ala Thr Ser Val Val  Asn Lys Met Glu Tyr  Ser Ser Leu 
    1415                 1420                 1425             


Asn Lys  Met Glu Lys Ser Ile  Asp Asn Leu Met Val  Lys Glu Ile 
    1430                 1435                 1440             


Cys Ile  Lys Leu Lys Ile Asp  Arg Leu Gly Asn Ile  Ser Lys Ala 
    1445                 1450                 1455             


<210>  1273
<211>  1458
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1273

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Gln Asp Phe Glu Ser Lys Ala Gly Lys Ile Ser Gly 
            20                  25                  30          


Ala Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Thr Phe 
        35                  40                  45              


Glu Thr Gly Gln Ser Tyr Ser Gln Thr Ala Asp Arg Thr His Tyr Arg 
    50                  55                  60                  


Ser Ile Arg Arg Leu Tyr Gln Arg Thr Lys Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Ser Leu Asn Val Leu Gly Phe Leu Pro Glu His Tyr Lys Asn 
                85                  90                  95      


Glu Ile Asp Phe Glu Gln Arg Lys Gly Gln Phe Tyr Pro Asn Lys Glu 
            100                 105                 110         


Pro Lys Ile Ala Tyr Tyr Pro Glu Gln Val Ala Ser Lys Val Lys Tyr 
        115                 120                 125             


Gln Phe Leu Phe Lys Ser Ser Phe Leu Glu Met Val Glu Asp Phe Lys 
    130                 135                 140                 


Arg Arg Gly Tyr Asn Gly Ala Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr 
145                 150                 155                 160 


Leu Arg Lys Lys Ala Leu Glu Lys Lys Ile Glu Lys Glu Glu Leu Ala 
                165                 170                 175     


Trp Leu Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg 
            180                 185                 190         


Gly Glu Glu Glu Asp Thr Asn Ser Asn Lys Lys Glu Glu Phe Tyr Glu 
        195                 200                 205             


Leu Lys Val Ser Ser Val Glu Gln Thr His Asp Thr Ser Pro Ser Gly 
    210                 215                 220                 


Thr Trp Tyr Asn Val His Leu Gln Asn Gly Trp Val Tyr Arg Arg Lys 
225                 230                 235                 240 


Ser Lys Asp Ser Leu Lys Asp Trp Glu Gly Leu Asn Lys Lys Phe Ile 
                245                 250                 255     


Val Thr Thr His Leu Asn Ala Asp Gly Ser Glu Lys Leu Asp Lys Asp 
            260                 265                 270         


Gly Asn Val Ser Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp Trp Gly 
        275                 280                 285             


Leu Val Lys Lys Arg Thr Glu Ser Val Leu Glu Lys Ser Gly Leu His 
    290                 295                 300                 


Val Gly Glu Phe Ile Tyr Glu Ser Leu Leu Glu Asn Pro Glu Gln Lys 
305                 310                 315                 320 


Ile Arg Gly Ser Leu Ile Lys Thr Ile Glu Arg Lys Tyr Tyr Lys Lys 
                325                 330                 335     


Glu Leu Glu Ala Ile Leu Asp Lys Gln Thr Ser Phe His Arg Glu Leu 
            340                 345                 350         


Arg Asn Asp Ala Leu Tyr Lys Glu Ala Thr Leu Glu Leu Tyr Pro Lys 
        355                 360                 365             


Asn Ser Ala His Arg Ser Asn Ile Lys Gln Arg Gly Phe Lys Tyr Leu 
    370                 375                 380                 


Phe Ile Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Gln Lys 
385                 390                 395                 400 


His Leu Ile Ala His Cys Gln Tyr Glu Lys Arg Thr Tyr Lys Asn Asn 
                405                 410                 415     


Gly Gly Glu Thr Ile Glu Val Ser Leu Gln Gly Ala Pro Lys Ser His 
            420                 425                 430         


Pro Ser Phe Gln Glu Phe Arg Ile Trp Gln Phe Ile Ser Asn Leu Arg 
        435                 440                 445             


Phe Tyr Asp Lys Glu Glu Ile Val Glu Gly Gln Glu Val Ser Ile Thr 
    450                 455                 460                 


Glu Lys Leu Leu Lys Ser Asp Ser Asp Phe Ala Glu Leu Tyr His Trp 
465                 470                 475                 480 


Leu Ser His Gln Arg Glu Val Asp Gln Asn Lys Thr Leu Lys Tyr Phe 
                485                 490                 495     


Thr Gln Lys Lys Leu Ile Pro Lys Gln Lys Lys Asp Asn Pro Thr Ile 
            500                 505                 510         


Arg Trp Asn Phe Pro Glu Asp Lys Lys Tyr Glu Met Gly Glu Phe Lys 
        515                 520                 525             


Ala Gln Leu Leu Ser Arg Leu Glu Lys Val Arg Asn Cys Gln Pro Asp 
    530                 535                 540                 


Lys Phe Leu Thr Gln Lys Asn Glu Leu Asp Leu Trp His Ile Ile Tyr 
545                 550                 555                 560 


Ser Val Thr Asp Lys Thr Glu Phe Glu Lys Ala Leu Val Thr Phe Ala 
                565                 570                 575     


Glu Lys Arg Glu Leu Asp Ser Glu Ser Phe Val Glu Asn Phe Lys Lys 
            580                 585                 590         


Phe Pro Pro Phe Lys Asn Asp Tyr Ala Ser Tyr Ser Leu Lys Ala Ile 
        595                 600                 605             


Lys Lys Ile Leu Pro Leu Met Arg Arg Gly Lys Tyr Trp Lys Ala Glu 
    610                 615                 620                 


Asn Leu Gly Pro Gln Thr Leu Gln Arg Ile Glu Lys Ile Val Asn Gly 
625                 630                 635                 640 


Glu Ala Asp Asp Lys Ile Ser Ile Arg Ala Arg Lys Leu Ala Leu His 
                645                 650                 655     


Leu Asn Asp Thr Lys Ser Phe Gln Gly Leu Pro Thr Ser Leu Ala Cys 
            660                 665                 670         


Tyr Val Ala Tyr Asn Arg His Ser Glu Asn Glu Glu Asn Gln Lys Trp 
        675                 680                 685             


Gln Thr Pro Ser Asp Ile Asp Lys Tyr Leu Lys Asp Phe Lys Gln His 
    690                 695                 700                 


Ser Leu Arg Asn Pro Ile Val Glu Gln Leu Ile Thr Glu Ala Leu Arg 
705                 710                 715                 720 


Val Val Arg Asp Ile Trp Lys His Tyr Gly Gln Gly Gln Lys Asp Phe 
                725                 730                 735     


Phe Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys Asn Pro Ala 
            740                 745                 750         


Ile Lys Arg Lys Lys Leu Ser Gln Lys Ile Gln Glu Asn Glu Asn Thr 
        755                 760                 765             


Asn Tyr Arg Ile Lys Glu Val Leu Arg Asn Leu Lys Glu Asn Asn Ile 
    770                 775                 780                 


Asp Ala Lys Pro Tyr Ser Pro Ser His Gln Glu Ile Leu Lys Ile Tyr 
785                 790                 795                 800 


Glu Glu Gly Val Tyr Gln Ser Ile Glu Asn Val Glu Asp Glu Ile Glu 
                805                 810                 815     


Lys Ile Arg Lys Asn Asn Thr Pro Ser Ser Lys Asp Ile Asn Arg Tyr 
            820                 825                 830         


Arg Leu Trp Leu Glu Gln Gly Tyr Ile Ser Pro Tyr Thr Gly Glu Val 
        835                 840                 845             


Ile Pro Leu Ser Gln Leu Phe Ser Asn Gln Tyr Glu Ile Glu His Ile 
    850                 855                 860                 


Ile Pro Arg Ser Arg Phe Phe Asp Asp Ser Leu Ala Asn Lys Val Ile 
865                 870                 875                 880 


Cys Glu Ser Ser Val Asn Ala Leu Lys Asp Asn Lys Thr Ala Leu Glu 
                885                 890                 895     


Phe Ile Thr Asn His Gly Asn Glu Lys Val Glu Phe Ala Gly Lys Thr 
            900                 905                 910         


Val Glu Ile Leu Ser Ser Asp Lys Tyr Thr Glu His Cys Lys Arg Tyr 
        915                 920                 925             


Phe Lys Arg Asn Arg Thr Lys Leu Lys Asn Leu Leu Ser Tyr Asp Val 
    930                 935                 940                 


Pro Glu Gly Phe Ile Asn Arg Gln Leu Asn Asp Ser Arg Phe Ile Ser 
945                 950                 955                 960 


Lys Tyr Val Arg Asn Leu Leu Ser Asn Ile Val Arg Glu Glu Glu Glu 
                965                 970                 975     


Arg Glu Ala Ile Ser Lys His Ile Leu Thr Ile Pro Gly Ser Ile Thr 
            980                 985                 990         


Ser Gln Leu Lys Gln Asp Trp Gly  Leu Asn Glu Lys Trp  Asn Glu Val 
        995                 1000                 1005             


Val Thr  His Arg Phe Glu Arg  Leu Asn Asn Leu Thr  Gly Thr Thr 
    1010                 1015                 1020             


Asp Phe  Gly Phe Trp Asp Asn  Lys Ile Asn Ala Phe  Arg Ile Thr 
    1025                 1030                 1035             


Val Pro  Lys Glu Leu Ser Lys  Gly Phe Asn Lys Lys  Arg Ile Asp 
    1040                 1045                 1050             


His Arg  His His Thr Leu Asp  Ala Leu Val Ile Ala  Ala Cys Thr 
    1055                 1060                 1065             


Lys Asp  His Val Asn Tyr Ile  Thr Ser Leu Asn Thr  Glu Arg Asn 
    1070                 1075                 1080             


Asn His  Ser Leu Val Ser Lys  Leu Arg Asn Thr Lys  Lys Val Leu 
    1085                 1090                 1095             


Ile Asn  Gly Lys Glu Arg Arg  Ile Ala Lys Ala Tyr  Lys Leu Pro 
    1100                 1105                 1110             


Trp Ser  Ser Phe Pro Leu Asp  Ala Lys His Gln Leu  Glu Thr Thr 
    1115                 1120                 1125             


Val Ile  Ser Phe Lys Gln Asn  Leu Arg Val Ile Asn  Lys Ser Ser 
    1130                 1135                 1140             


Asn Lys  Thr Trp Gln Trp Arg  Asn Val Asn Gly Glu  Gln Lys Lys 
    1145                 1150                 1155             


Lys Leu  Ile Pro Gln Thr Lys  Gly Asp Ser Trp Ala  Ile Arg Lys 
    1160                 1165                 1170             


Ser Met  His Lys Ala Thr Val  Ser Ala Lys Val Glu  Ser Val Gly 
    1175                 1180                 1185             


Lys Gly  Lys Ile Lys Thr Thr  Asn Arg Thr Pro Leu  Gly Glu Ile 
    1190                 1195                 1200             


Lys Gly  Glu Lys His Val Glu  Lys Ile Tyr Asp Ala  Arg Ile Arg 
    1205                 1210                 1215             


Glu Val  Ile Ile Pro Asn His  Leu Asn Lys Tyr Leu  Asp Glu Lys 
    1220                 1225                 1230             


Gly Lys  Pro Asn Tyr Glu Ala  Ala Phe Ser Gln Ser  Gly Ile Lys 
    1235                 1240                 1245             


Gln Leu  Asn Glu Asn Ile Glu  Val Leu Asn Glu Gly  Lys Pro His 
    1250                 1255                 1260             


Ala Pro  Ile Tyr Lys Val Pro  Leu Phe Glu Val Gly  Ser Lys Phe 
    1265                 1270                 1275             


Pro Val  Ser Glu Asp Ala Lys  Ser Pro Lys Asn Gln  Lys Tyr Val 
    1280                 1285                 1290             


Glu Ala  Asp Lys Gly Thr Asn  Leu Phe Phe Ala Val  Tyr Trp Asn 
    1295                 1300                 1305             


Glu Glu  Lys Gln Lys Arg Asn  Phe Glu Thr Val Pro  Leu His Gln 
    1310                 1315                 1320             


Val Ile  Val His Gln Lys Gln  Val Ala Ser Leu Pro  Thr Glu Gln 
    1325                 1330                 1335             


Arg Thr  Ser Val Pro Ile Lys  Pro Glu Leu Gly Leu  Phe Leu Phe 
    1340                 1345                 1350             


Ser Leu  Ser Pro Asn Asp Leu  Val Tyr Val Pro Thr  Glu Glu Glu 
    1355                 1360                 1365             


Ile Glu  Asn Pro His Leu Val  Asp Leu Lys Asn Leu  Ser Lys Asp 
    1370                 1375                 1380             


Gln Thr  Asn Arg Ile Tyr Lys  Met Val Ser Ser Thr  Gly Asn Glu 
    1385                 1390                 1395             


Cys His  Phe Ile Pro His His  Ile Ser Arg Leu Ile  Met Lys Tyr 
    1400                 1405                 1410             


Asn Ala  Lys Gln Lys Val Gly  Glu Phe Gly Ser Leu  Asn Lys Ala 
    1415                 1420                 1425             


Glu Lys  Ala Ile Thr Gly Glu  Thr Ile Lys Glu Lys  Cys Leu Lys 
    1430                 1435                 1440             


Ile Gln  Ile Asn Ser Leu Gly  Lys Leu Lys Thr Glu  Arg Ile Ala 
    1445                 1450                 1455             


<210>  1274
<211>  1459
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1274

Met Lys Phe Asn Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Glu Asp Tyr Glu Asn Lys Lys Gly Lys Ile Ile Ala 
            20                  25                  30          


Ala Gly Ser Arg Ile Ile Pro Met Thr Gln Asp Ile Leu Asp Lys Phe 
        35                  40                  45              


Gly Ser Gly Ala Ile Thr Glu Thr Gln Thr Ala Leu Arg Thr Asp Tyr 
    50                  55                  60                  


Arg Ser Lys Arg Lys Leu Ile Gln Arg Phe Leu Leu Arg Arg Glu Arg 
65                  70                  75                  80  


Leu His Arg Val Leu Asn Val Leu Asp Phe Leu Pro Lys His Tyr Ala 
                85                  90                  95      


Glu Ser Ile Asp Phe Glu Lys Tyr Phe Gly Lys Phe Leu Pro Asn Thr 
            100                 105                 110         


Glu Pro Lys Leu Ala Tyr Asn Gln Asn Gln Phe Ile Phe Gln Asn Ser 
        115                 120                 125             


Phe Leu Glu Met Leu Lys Glu Phe Gln Glu Lys His Pro Glu Leu Leu 
    130                 135                 140                 


Thr Asn Asn Lys Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg 
145                 150                 155                 160 


Lys Lys Ala Leu Asn Gln Arg Ile Glu Lys Glu Glu Leu Ala Trp Leu 
                165                 170                 175     


Leu Leu Gln Phe Asn Gln Lys Arg Gly Tyr Asn Gln Leu Arg Asp Glu 
            180                 185                 190         


Glu Glu Glu Ser Val Pro Asn Lys Gln Val Glu Phe Tyr Ser Leu Lys 
        195                 200                 205             


Ile Ile Asp Val Val Ala Asp Gln Pro Lys Glu Gly Arg Asp Lys Arg 
    210                 215                 220                 


Trp Tyr Ser Ile Asn Leu Glu Asn Gly Trp Thr Tyr Arg Arg Glu Ser 
225                 230                 235                 240 


Lys Thr Pro Leu Phe Asn Trp Glu Gly Lys Thr Lys Asp Phe Ile Val 
                245                 250                 255     


Thr Thr Asp Leu Asn Gln Asp Gly Ser Ile Lys Lys Asp Lys Glu Gly 
            260                 265                 270         


Lys Glu Lys Arg Ser Phe Arg Ala Pro Ser Glu Gly Asp Trp Thr Leu 
        275                 280                 285             


Leu Lys Lys Lys Thr Glu Lys Glu Ile Tyr Glu Ser Gly Lys Thr Leu 
    290                 295                 300                 


Gly Thr Tyr Ile Tyr Asp Ala Leu Leu Asn Thr Pro Asp Leu Lys Ile 
305                 310                 315                 320 


Lys Gly Asp Phe Ile Arg Thr Ile Asp Arg Ser Ile Tyr Lys Glu Glu 
                325                 330                 335     


Leu Gln Gln Ile Ile Asn Lys Gln Lys Glu Tyr His Leu Glu Leu Asn 
            340                 345                 350         


Ser Ser Lys Leu Tyr Lys Ala Cys Leu Glu Glu Leu Tyr Pro Lys Asn 
        355                 360                 365             


Glu Ile Tyr Lys Ser Ala Leu Lys Asn Lys Asp Ile Ala Tyr Leu Phe 
    370                 375                 380                 


Ile Asn Asp Ile Leu Tyr Tyr Gln Arg Pro Leu Lys Ala Pro Lys Tyr 
385                 390                 395                 400 


Asn Ile Gly Lys Cys Thr Tyr Glu Ser Arg Thr Phe Lys Lys Glu Asp 
                405                 410                 415     


Lys Lys Ile Arg Glu Tyr Ile Pro Ala Val Ala Lys Ser His Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Arg Leu Trp Gln Trp Ile Lys Asn Leu Lys Ile Tyr 
        435                 440                 445             


Glu Lys Leu Ser Ser Asp Asn Cys Thr Asp Phe Phe Leu Thr Asn Glu 
    450                 455                 460                 


Gln Asp Phe Glu Asn Leu Phe Glu Phe Leu Trp Asn Lys Lys Glu Ile 
465                 470                 475                 480 


Asp His Lys Gly Ile Leu Glu Tyr Leu Val Lys Thr Lys Phe Pro Asp 
                485                 490                 495     


Leu Lys Pro Lys Gln Val Lys Thr Lys Val Lys Glu Tyr Arg Trp Asn 
            500                 505                 510         


Tyr Val Tyr Asp His Glu Lys Asp Glu Ser Lys Thr Tyr Pro Cys Gly 
        515                 520                 525             


Glu Thr Tyr Phe Leu Leu Lys Asn Lys Leu Asp Lys Ile Tyr Asn Ala 
    530                 535                 540                 


Gln Asp Asp Phe Leu Asp Lys Glu Arg Leu Tyr Asn Leu Trp His Ile 
545                 550                 555                 560 


Ile Tyr Ser Val Thr Asp Lys Lys Gln Tyr Glu Lys Ala Leu Ala Lys 
                565                 570                 575     


Phe Ala Leu Lys Asn His Leu Asp Thr Lys Ser Phe Val Asn Thr Phe 
            580                 585                 590         


Lys Arg Ile Pro Pro Phe Lys Asn Asp Tyr Ala Ile Tyr Ser Leu Lys 
        595                 600                 605             


Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Val Gly Lys Tyr Trp Asn 
    610                 615                 620                 


Tyr Asp Asp Ile Asp Pro Ile Thr Lys Gln Lys Ile Gln Cys Ile Ile 
625                 630                 635                 640 


Thr Gly Glu Glu Asn Asp Thr Ile Ser Thr Leu Val Arg Glu Lys Thr 
                645                 650                 655     


Asn His Phe Ser Asn Glu Asn Asp Phe Lys Asn Leu Pro Leu Trp Leu 
            660                 665                 670         


Ala Gly Tyr Ile Val Tyr Asn Ser His Asn Glu Ser Ser Glu Ile Glu 
        675                 680                 685             


Lys Trp Lys Ser Pro Gln Asp Ile Asp Thr Phe Leu Lys Lys Phe Lys 
    690                 695                 700                 


Gln His Ala Leu Arg Asn Pro Ile Val Glu Gln Val Val Thr Glu Thr 
705                 710                 715                 720 


Leu Arg Val Val Arg Asp Ile Trp Ser Lys Lys Gly Gln Gly Lys Lys 
                725                 730                 735     


Asp Phe Phe Asn Glu Ile His Ile Glu Leu Gly Lys Asp Leu Lys Asn 
            740                 745                 750         


Pro Val Asp Lys Arg Arg Lys Leu Ser Ser Thr Asn Thr Lys Asn Glu 
        755                 760                 765             


Ser Thr Asn Thr Arg Ile Lys Ala Leu Leu Ile Glu Leu Phe Thr Gln 
    770                 775                 780                 


Gly Val Glu Asn Val Lys Pro Asn Ser Pro Lys His Gln Glu Ile Leu 
785                 790                 795                 800 


Lys Ile Phe Glu Glu Gly Ala Leu Asn Ser Gly Ile Glu Ile Glu Glu 
                805                 810                 815     


Asp Ile Ile Lys Ile Ser Asn Thr Asp Gln Pro Thr Lys Lys Gln Leu 
            820                 825                 830         


Glu Leu Tyr Ile Leu Trp Leu Glu His Lys Tyr Lys Ser Pro Tyr Thr 
        835                 840                 845             


Gly Gln Ser Ile Pro Leu Ser Lys Leu Phe Thr Pro Asn Tyr Glu Ile 
    850                 855                 860                 


Glu His Ile Ile Pro Arg Ser Lys Tyr Tyr Asp Asp Asn Leu Ser Asn 
865                 870                 875                 880 


Lys Val Ile Cys Glu Thr Glu Ile Asn Arg Leu Lys Asn Asn Gln Leu 
                885                 890                 895     


Gly Tyr Glu Phe Ile Asn Asn His Glu Ile Arg Glu Val Thr Leu Glu 
            900                 905                 910         


Glu Gly Lys Thr Ile Arg Leu Leu Ser Pro Glu Gln Tyr Trp Asn Phe 
        915                 920                 925             


Val Thr Thr Tyr Tyr Ala Lys Asn Lys Ala Lys Arg Glu Asn Leu Leu 
    930                 935                 940                 


Leu Lys Glu Leu Pro Val Gln Met Val Glu Lys Gln Leu Asn Asp Thr 
945                 950                 955                 960 


Arg Tyr Val Ser Lys Tyr Val Met Gln Leu Leu Ser Lys Ile Val Arg 
                965                 970                 975     


Lys Glu Asp Gly Thr Asp Asn Gly Ile Asn Ser Thr Asn Ile Leu Ser 
            980                 985                 990         


Ile Ser Gly Lys Met Thr Arg Ala  Leu Lys Asn Asp Trp  Asp Leu Asn 
        995                 1000                 1005             


Asp Val  Trp Asn Asp Leu Leu  Leu Tyr Arg Phe Glu  Arg Leu Asn 
    1010                 1015                 1020             


Thr Ile  Thr Lys Thr Asn Leu  Phe Thr Thr Tyr Ser  Glu Gln Lys 
    1025                 1030                 1035             


Gln Lys  Arg Met Pro Ile Val  Pro Asp Gln Tyr Ala  Lys Gly Phe 
    1040                 1045                 1050             


His Lys  Lys Arg Leu Asp His  Arg His His Ala Leu  Asp Ala Ile 
    1055                 1060                 1065             


Val Ile  Ala Cys Ile Thr Arg  Asn His Ile Asn Tyr  Met Asn Asn 
    1070                 1075                 1080             


Gln Asn  Ile Gln Gly Glu Lys  Leu Lys Lys Asn Ala  Ile Glu Lys 
    1085                 1090                 1095             


Glu Asn  Tyr Arg Glu Ile Leu  Gln Lys Ser Arg Leu  Asp Ile Arg 
    1100                 1105                 1110             


Ala Gln  Leu Cys Glu Lys Lys  Tyr Asn Asp Asn Asp  Pro Thr Lys 
    1115                 1120                 1125             


Tyr Asn  Trp Val Phe Ile Lys  Pro Trp Ala Asp Phe  Thr Glu Asn 
    1130                 1135                 1140             


Thr Lys  Asp Ile Leu Glu Ser  Ile Ile Pro Ser Phe  Lys Gln Asn 
    1145                 1150                 1155             


Leu Arg  Ile Ile Asn Lys Thr  Val Asn Tyr Tyr Glu  Lys Tyr Asn 
    1160                 1165                 1170             


Glu Asn  Gly Lys Lys Val Lys  Val Arg Gln Ile Lys  Gly Asp Asn 
    1175                 1180                 1185             


Trp Ala  Ile Arg Lys Pro Leu  His Lys Glu Thr Ile  Ser Gly Lys 
    1190                 1195                 1200             


Val His  Leu Ser Tyr Lys Thr  Pro Ala Lys Gly Lys  Ile Leu Thr 
    1205                 1210                 1215             


Ala Thr  Arg Lys Val Leu Asp  Ser Ser Met Asp Phe  Lys Ser Ile 
    1220                 1225                 1230             


Glu Lys  Ile Thr Asp Thr Gly  Ile Gln Lys Ile Leu  Lys Asn Tyr 
    1235                 1240                 1245             


Leu Glu  Tyr Lys Lys Ser Pro  Glu Leu Ala Phe Ser  Pro Glu Gly 
    1250                 1255                 1260             


Ile Glu  Glu Leu Asn Ala Thr  Ile Thr Lys Tyr Asn  Asn Gly Phe 
    1265                 1270                 1275             


His His  Lys Pro Ile Tyr Lys  Val Arg Ile Tyr Glu  Glu Gly Val 
    1280                 1285                 1290             


Lys Phe  Pro Leu Gly Asn Lys  Gly Asn Lys Lys Ser  Lys Tyr Val 
    1295                 1300                 1305             


Glu Ala  Glu Lys Gly Thr Asn  Leu Phe Phe Ala Ile  Tyr Lys Asp 
    1310                 1315                 1320             


Gln Glu  Asn Asn Arg Tyr Phe  Glu Thr Ile Pro Phe  Asn Asp Val 
    1325                 1330                 1335             


Leu Glu  Thr Leu Lys Lys Gly  Met Ser Ala Val Pro  Glu Lys Asn 
    1340                 1345                 1350             


Gln Glu  Glu Asn Thr Leu Val  Phe Ala Leu Ser Pro  Gln Asp Leu 
    1355                 1360                 1365             


Val Phe  Val Pro Thr Leu Asp  Glu Ile Glu Asn Pro  Asp Ile Val 
    1370                 1375                 1380             


Asp Phe  Lys Asn Leu Thr Lys  Asp Gln Thr Asn Arg  Ile Tyr Lys 
    1385                 1390                 1395             


Met Val  Ser Ser Ser Gly Lys  Gln Cys Phe Phe Ile  Lys Ala Glu 
    1400                 1405                 1410             


Val Ala  Thr Ser Ile Val Asn  Lys Met Glu Phe Ser  Thr Ser Asn 
    1415                 1420                 1425             


Lys Met  Glu Asn Ser Ile Glu  Gly Ile Gln Ile Lys  Ser Asn Cys 
    1430                 1435                 1440             


Trp Lys  Leu Lys Leu Asp Arg  Leu Gly Thr Ile Thr  Arg Val Ile 
    1445                 1450                 1455             


Lys 
    


<210>  1275
<211>  1460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1275

Met Thr Lys Phe Asn Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Val Asn Glu Asp Cys Glu Asn Lys Lys Gly Lys Ile Leu 
            20                  25                  30          


Ala Ala Gly Ser Arg Ile Ile Pro Met Thr Gln Asp Ile Leu Asp Lys 
        35                  40                  45              


Phe Gly Ser Gly Ala Ala Thr Glu Thr Gln Thr Ala Leu Arg Thr Asp 
    50                  55                  60                  


Tyr Arg Ser Lys Arg Lys Leu Ile Gln Arg Phe Leu Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Arg Val Leu Asn Val Leu Asp Phe Leu Pro Lys His Tyr 
                85                  90                  95      


Ala Glu Ser Ile Asp Phe Glu Lys Tyr Leu Gly Lys Phe Leu Pro Asn 
            100                 105                 110         


Thr Glu Pro Lys Leu Val Tyr Asn Gln Asn Gln Phe Ile Phe Gln Asn 
        115                 120                 125             


Ser Phe Phe Glu Met Leu Lys Glu Phe Gln Glu Lys His Pro Glu Leu 
    130                 135                 140                 


Phe Leu Asn Asn Gln Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu 
145                 150                 155                 160 


Arg Lys Lys Ala Leu Ser Gln Lys Ile Glu Lys Glu Glu Leu Ala Trp 
                165                 170                 175     


Leu Leu Leu Gln Phe Asn Gln Lys Arg Gly Tyr Asn Gln Leu Arg Asp 
            180                 185                 190         


Glu Glu Glu Glu Asn Thr Pro Asp Lys Leu Val Glu Phe Tyr Ser Leu 
        195                 200                 205             


Lys Ile Ile Lys Val Thr Ala Asp Glu Ala Lys Glu Gly Ile Asn Lys 
    210                 215                 220                 


Arg Trp Tyr Ser Ile Asn Leu Glu Asn Gly Trp Ile Tyr Arg Arg Glu 
225                 230                 235                 240 


Ser Lys Thr Pro Leu Phe Asn Trp Glu Gly Lys Thr Lys Asn Phe Ile 
                245                 250                 255     


Val Thr Thr Asp Leu Asn Gln Asp Gly Ser Ile Lys Lys Asp Lys Glu 
            260                 265                 270         


Gly Lys Glu Lys Arg Ser Phe Arg Ala Pro Ser Glu Gly Asp Trp Thr 
        275                 280                 285             


Leu Leu Lys Lys Lys Thr Glu Lys Glu Ile Tyr Glu Ser Gly Lys Thr 
    290                 295                 300                 


Leu Gly Thr Tyr Ile Tyr Asp Ala Leu Leu Asn Asn Pro Asp Leu Lys 
305                 310                 315                 320 


Ile Lys Gly Ala Leu Ile Lys Thr Ile Asp Arg Ser Ile Tyr Lys Glu 
                325                 330                 335     


Glu Leu Gln Gln Ile Ile Asn Lys Gln Lys Glu Tyr His Ser Glu Leu 
            340                 345                 350         


Asn Asn Ser Lys Leu Tyr Lys Ala Cys Leu Glu Glu Leu Tyr Pro Lys 
        355                 360                 365             


Asn Glu Ile Tyr Lys Asn Glu Leu Lys Asn Lys Asp Ile Ala Tyr Leu 
    370                 375                 380                 


Phe Ile Asn Asp Ile Leu Tyr Tyr Gln Arg Pro Leu Lys Ala Pro Lys 
385                 390                 395                 400 


Tyr Asn Ile Gly Lys Cys Ser Tyr Glu Ser Arg Thr Phe Lys Lys Glu 
                405                 410                 415     


Asp Lys Lys Ile Thr Glu Phe Ile Pro Ala Val Ala Lys Ser His Pro 
            420                 425                 430         


Leu Phe Gln Glu Phe Arg Leu Trp Gln Trp Ile Lys Asn Leu Lys Ile 
        435                 440                 445             


Tyr Glu Lys Leu Thr Asn Asp Asn Tyr Thr Asp Phe Phe Leu Thr Asn 
    450                 455                 460                 


Glu Arg Asp Ile Glu Lys Leu Phe Asp Phe Leu Trp Asn Lys Lys Glu 
465                 470                 475                 480 


Ile Asp His Lys Gly Ile Leu Glu Tyr Leu Ile Lys Thr Lys Phe Pro 
                485                 490                 495     


Asp Leu Lys Pro Lys Gln Val Lys Ser Lys Val Lys Glu Tyr Arg Trp 
            500                 505                 510         


Asn Tyr Ile Tyr Asp His Glu Lys Asp Glu Ser Lys Asn Tyr Pro Cys 
        515                 520                 525             


Gly Glu Thr Tyr Phe Leu Leu Lys Asn Lys Leu Asp Lys Ile Thr Ser 
    530                 535                 540                 


Ile Gln Asp Asp Phe Leu Asn Lys Lys Arg Leu Glu Asn Leu Trp His 
545                 550                 555                 560 


Ile Ile Tyr Ser Val Thr Asp Lys Lys Gln Tyr Glu Lys Ala Leu Ala 
                565                 570                 575     


Lys Phe Ala Leu Lys Asn Gln Leu Asp Thr Lys Ser Phe Val Asn Thr 
            580                 585                 590         


Phe Lys Arg Ile Pro Ala Phe Lys Asn Asp Tyr Ala Ile Tyr Ser Leu 
        595                 600                 605             


Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Val Gly Lys Tyr Trp 
    610                 615                 620                 


Asn Tyr Asp Asp Ile Asp Pro Ile Thr Lys Gln Lys Ile Gln Cys Ile 
625                 630                 635                 640 


Ile Thr Gly Glu Glu Asn Asp Thr Ile Ser Thr Leu Val Arg Glu Lys 
                645                 650                 655     


Thr Asn His Phe Ser Asn Glu Asn Asp Phe Lys Asn Leu Ser Leu Trp 
            660                 665                 670         


Leu Ala Gly Tyr Ile Val Tyr Asn Ser His Asn Glu Ser Ser Glu Ile 
        675                 680                 685             


Glu Lys Trp Lys Ser Pro Gln Asp Ile Asp Thr Phe Leu Lys Lys Phe 
    690                 695                 700                 


Lys Gln His Ala Leu Arg Asn Pro Ile Val Glu Gln Val Val Thr Glu 
705                 710                 715                 720 


Thr Leu Arg Val Val Arg Asp Ile Trp Leu Lys Lys Gly Gln Gly Lys 
                725                 730                 735     


Lys Asp Phe Phe Asn Glu Ile His Ile Glu Leu Gly Lys Asp Leu Lys 
            740                 745                 750         


Asn Pro Val Asp Lys Arg Arg Lys Ile Ser Ser Thr Asn Ser Lys Asn 
        755                 760                 765             


Glu Thr Thr Asn Ile Arg Ile Lys Ala Leu Leu Lys Glu Leu Leu Lys 
    770                 775                 780                 


Glu Gly Ile Glu Asn Ile Lys Pro Asn Ser Pro Lys Gln Gln Glu Ile 
785                 790                 795                 800 


Leu Lys Ile Phe Glu Glu Gly Ile Leu Asn Ser Gly Ile Asp Ile Glu 
                805                 810                 815     


Glu Glu Ile Ile Lys Ile Ser Gln Ile Ala Gln Pro Thr Lys Lys Gln 
            820                 825                 830         


Leu Gln Tyr Tyr Arg Asn Trp Leu Glu Gln Lys Tyr Ile Ser Pro Tyr 
        835                 840                 845             


Thr Gly Gln Phe Ile Pro Leu Ser Lys Leu Phe Thr Ser Lys Tyr Glu 
    850                 855                 860                 


Ile Glu His Ile Ile Pro Arg Ser Lys Tyr Tyr Asp Asp Ser Leu Ser 
865                 870                 875                 880 


Asn Lys Val Ile Cys Glu Thr Glu Ile Asn Arg Leu Lys Asn Asn Gln 
                885                 890                 895     


Leu Gly Tyr Glu Phe Ile Asn Asn His Glu Ile Arg Glu Ile Thr Leu 
            900                 905                 910         


Glu Glu Gly Lys Thr Ile Arg Leu Leu Ser Pro Lys Gln Tyr Leu Asp 
        915                 920                 925             


Phe Val Thr Thr Tyr Tyr Gly Lys Asn Lys Ala Lys Arg Glu Asn Leu 
    930                 935                 940                 


Leu Leu Lys Glu Ile Pro Ile Gln Met Ile Glu Asn Gln Leu Asn Asp 
945                 950                 955                 960 


Thr Arg Tyr Val Ser Lys Tyr Val Met Gln Leu Leu Ser Lys Ile Val 
                965                 970                 975     


Arg Lys Glu Asp Gly Thr Asp Asn Gly Thr Asn Ser Ile Asn Ile Leu 
            980                 985                 990         


Ser Val Ser Gly Lys Ile Thr Arg  Val Leu Lys Thr Asp  Trp Gly Leu 
        995                 1000                 1005             


Asn Asp  Val Trp Asn Asp Leu  Leu Cys Tyr Arg Phe  Glu Arg Leu 
    1010                 1015                 1020             


Asn Lys  Ile Thr Asn Thr Asn  Leu Phe Thr Thr Tyr  Ser Glu Gln 
    1025                 1030                 1035             


Lys Gln  Lys Ile Ile Pro Ile  Val Pro Asp Gln Tyr  Ala Lys Gly 
    1040                 1045                 1050             


Phe His  Lys Lys Lys Leu Asp  His Arg His His Ala  Leu Asp Ala 
    1055                 1060                 1065             


Ile Leu  Ile Ala Cys Ile Thr  Arg Asn His Ile Asn  Tyr Met Asn 
    1070                 1075                 1080             


Asn Gln  Asn Ile Gln Gly Glu  Lys Leu Lys Asn Phe  Lys Ile Asp 
    1085                 1090                 1095             


Lys His  Asn Tyr His Glu Ile  Phe Gln Lys Ser Arg  Leu Asp Met 
    1100                 1105                 1110             


Arg Ala  Gln Leu Cys Glu Lys  Lys Tyr Asn Asp His  Asp Ser Thr 
    1115                 1120                 1125             


Lys Tyr  Thr Trp Val Phe Thr  Lys Pro Trp Ala Asp  Phe Thr Glu 
    1130                 1135                 1140             


Asn Ile  Lys Asp Thr Leu Glu  Phe Ile Ile Pro Ser  Phe Lys Gln 
    1145                 1150                 1155             


Asn Leu  Arg Ile Ile Asn Lys  Thr Val Asn Tyr Tyr  Glu Lys Tyr 
    1160                 1165                 1170             


Asn Glu  Asn Gly Lys Lys Val  Lys Val Arg Gln Thr  Lys Gly Asp 
    1175                 1180                 1185             


Asn Trp  Ala Ile Arg Lys Pro  Leu His Lys Glu Thr  Ile Ser Gly 
    1190                 1195                 1200             


Lys Val  His Leu Leu Tyr Lys  Thr Pro Ala Lys Gly  Lys Ile Leu 
    1205                 1210                 1215             


Thr Ala  Thr Arg Lys Val Leu  Asp Ser Ser Met Asp  Phe Lys Ser 
    1220                 1225                 1230             


Ile Glu  Lys Ile Thr Asp Thr  Gly Ile Gln Lys Ile  Leu Lys Asn 
    1235                 1240                 1245             


Tyr Leu  Lys Tyr Lys Glu Ser  Pro Glu Leu Ala Phe  Ser Pro Glu 
    1250                 1255                 1260             


Gly Ile  Glu Glu Leu Asn Ile  Thr Ile Ala Lys Tyr  Asn Asp Gly 
    1265                 1270                 1275             


Phe Tyr  His Lys Pro Ile Tyr  Lys Val Arg Ile Tyr  Glu Glu Gly 
    1280                 1285                 1290             


Val Lys  Phe Pro Leu Gly Asn  Arg Gly Asn Lys Lys  Asn Lys Tyr 
    1295                 1300                 1305             


Val Glu  Ala Glu Lys Gly Thr  Asn Leu Phe Phe Ala  Ile Tyr Lys 
    1310                 1315                 1320             


Asp Gln  Glu Asn Asn Arg Tyr  Phe Glu Thr Ile Pro  Phe Asn Asp 
    1325                 1330                 1335             


Val Leu  Glu Thr Leu Lys Lys  Gly Met Ser Ala Val  Pro Glu Lys 
    1340                 1345                 1350             


Asn Gln  Glu Lys Asn Thr Leu  Val Phe Ala Leu Ser  Pro Gln Asp 
    1355                 1360                 1365             


Leu Val  Phe Val Pro Thr Leu  Asp Glu Ile Glu Asn  Pro Asp Ile 
    1370                 1375                 1380             


Val Asp  Phe Arg Asn Leu Thr  Lys Asp Gln Thr Asn  Arg Ile Tyr 
    1385                 1390                 1395             


Lys Met  Val Ser Ser Ser Gly  Lys Gln Cys Phe Phe  Ile Lys Ala 
    1400                 1405                 1410             


Glu Val  Ala Thr Ser Ile Val  Asn Lys Met Glu Phe  Thr Thr Ser 
    1415                 1420                 1425             


Asn Lys  Met Glu Asn Ser Ile  Glu Gly Ile Gln Ile  Lys Ser Asn 
    1430                 1435                 1440             


Cys Trp  Lys Leu Lys Leu Asp  Arg Leu Gly Thr Ile  Thr Arg Val 
    1445                 1450                 1455             


Ile Lys  
    1460 


<210>  1276
<211>  1462
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1276

Met Thr Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Ile Lys Gln Asn Phe Glu Asn Lys Gln Gly Glu Ile Leu 
            20                  25                  30          


Gly Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Asp 
        35                  40                  45              


Phe Gly Lys Gly Asn Ser Val Ser Gln Thr Ala Glu Arg Thr Lys Tyr 
    50                  55                  60                  


Arg Ser Val Arg Arg Leu Arg Glu Arg Phe Leu Leu Arg Arg Glu Arg 
65                  70                  75                  80  


Leu His Arg Val Leu Asn Leu Leu Asn Phe Leu Pro Gln His Tyr Ala 
                85                  90                  95      


Ser Gln Ile Asp Phe Glu Lys Arg Phe Gly Lys Phe Lys Val Glu Thr 
            100                 105                 110         


Glu Pro Lys Leu Ala Trp Lys Asn Ile Asp Gly Gln Phe Ser Phe Leu 
        115                 120                 125             


Phe Gln Thr Ser Phe Asn Glu Met Leu Glu Asp Phe Arg Val Asn Gly 
    130                 135                 140                 


Gln Asp Val Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys 
145                 150                 155                 160 


Lys Ala Leu Ser Lys Lys Ile Glu Lys Glu Glu Leu Ala Trp Ile Leu 
                165                 170                 175     


Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu 
            180                 185                 190         


Glu Glu Glu Asn Pro Asn Lys Leu Val Glu Phe Tyr Ser Leu Lys Val 
        195                 200                 205             


Val Asp Val Val Ala Asp Glu Pro Gln Lys Gly Lys Phe Asp Ile Trp 
    210                 215                 220                 


Tyr Ser Leu Ile Leu Glu Asn Gly Trp Val Tyr Arg Arg Ser Ser Lys 
225                 230                 235                 240 


Ile Pro Leu Phe Asp Trp Lys Asp Lys Thr Arg Asp Phe Ile Val Thr 
                245                 250                 255     


Thr Asp Leu Asn Asp Asp Gly Ser Ile Lys Lys Asp Lys Asp Gly Asn 
            260                 265                 270         


Glu Lys Arg Ser Phe Arg Ala Pro Ser Glu Asn Asp Trp Thr Leu Val 
        275                 280                 285             


Lys Lys Lys Thr Glu Gln Glu Ile Glu Gln Ser Arg Lys Thr Val Gly 
    290                 295                 300                 


Thr Tyr Ile Tyr Glu Ala Leu Leu Asp Asn Pro Lys Gln Lys Ile Lys 
305                 310                 315                 320 


Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Glu Glu Leu 
                325                 330                 335     


Arg Gln Ile Leu Glu Lys Gln Lys Glu Phe His Gln Glu Leu Gln Ser 
            340                 345                 350         


Asp Asp Leu Tyr Asn Asp Cys Val Arg Glu Leu Tyr Arg Asn Asn Glu 
        355                 360                 365             


Ala His Gln Leu Thr Leu Ser Lys Lys Asp Phe Val His Leu Leu Met 
    370                 375                 380                 


Asp Asp Leu Ile Phe Tyr Gln Arg Pro Leu Arg Ser Gln Lys Ser Ser 
385                 390                 395                 400 


Val Ser Asn Cys Thr Leu Glu Phe Arg Lys Tyr Lys Asp Glu Asn Gly 
                405                 410                 415     


Ala Glu His Thr Gln Tyr Leu Lys Ala Ile Pro Lys Ser Asn Pro Tyr 
            420                 425                 430         


Tyr Gln Glu Phe Arg Leu Trp Gln Trp Ile Tyr Asn Leu Asn Ile Tyr 
        435                 440                 445             


Arg Arg Glu Asp Glu Gln Asn Pro Ile Thr Gly Glu Phe Leu Ser Thr 
    450                 455                 460                 


Thr Lys Glu Phe Glu Asn Leu Phe Glu Phe Leu Asn Thr Arg Lys Glu 
465                 470                 475                 480 


Val Asp Gln Lys Ala Leu Leu Lys His Phe Lys Leu Asn Asp Lys Thr 
                485                 490                 495     


His Arg Trp Asn Phe Val Glu Asp Lys Lys Tyr Pro Cys Asn Glu Thr 
            500                 505                 510         


Lys Thr Met Ile Ser Ser Arg Leu Glu Lys Val Glu Asn Ile Ser Asp 
        515                 520                 525             


Asp Phe Leu Thr Arg Glu Ile Glu Gln Lys Ile Trp His Ile Ile Tyr 
    530                 535                 540                 


Ser Val Asn Asp Lys Ile Glu Tyr Glu Lys Ala Leu Lys Ser Phe Ala 
545                 550                 555                 560 


Lys Lys His His Leu Asp Glu Ser Ser Phe Phe Glu Ala Phe Arg Lys 
                565                 570                 575     


Phe Pro Pro Phe Lys Ser Glu Tyr Gly Ser Phe Ser Glu Lys Ala Val 
            580                 585                 590         


Lys Lys Leu Leu Pro Leu Met Arg Leu Gly Lys Tyr Trp Asn Tyr Ala 
        595                 600                 605             


Asp Ile Asp Lys Ser Ser Lys Glu Arg Ile Gln Lys Ile Ile Ser Gly 
    610                 615                 620                 


Glu Tyr Asp Glu Asn Ile Lys Asp Arg Val Arg Glu Lys Ser Val His 
625                 630                 635                 640 


Leu Thr Thr Glu Asn Asp Phe Gln Gly Leu Gln Leu Trp Leu Ala Gln 
                645                 650                 655     


Tyr Ile Val Tyr Gly Arg His Ser Glu Ala Ser Met Ile Gly Lys Trp 
            660                 665                 670         


Asn Ser Ala Asp Asp Leu Glu Glu Phe Leu Lys Asn Phe Lys Gln His 
        675                 680                 685             


Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu Arg 
    690                 695                 700                 


Val Val Lys Asp Ile Trp Leu Lys Tyr Gly Asn Gly Ala Lys Asp Phe 
705                 710                 715                 720 


Phe Asn Glu Ile His Ile Glu Leu Gly Arg Glu Met Lys Leu Pro Ala 
                725                 730                 735     


Lys Glu Arg Glu Asp Leu Thr Arg Lys Ile Thr Glu Asn Glu Asn Thr 
            740                 745                 750         


Asn Leu Arg Ile Lys Ala Leu Leu Ala Glu Met Met Asn Asp Ser Ser 
        755                 760                 765             


Val Glu Asn Val Arg Pro Phe Ser Pro Met Gln Gln Glu Ile Leu Lys 
    770                 775                 780                 


Ile Tyr Glu Asp Gly Val Leu Asn Ser Gly Ile Glu Ile Glu Asp Glu 
785                 790                 795                 800 


Tyr Leu Lys Ile Ser Lys Thr Ala Gln Pro Ser Pro Ser Asp Leu Lys 
                805                 810                 815     


Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Lys Ser Pro Tyr Thr Gly 
            820                 825                 830         


Gln Ile Ile Pro Leu Asn Lys Leu Phe Thr Pro Glu Tyr Glu Ile Glu 
        835                 840                 845             


His Ile Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys 
    850                 855                 860                 


Ile Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp Asn Tyr Ile Gly 
865                 870                 875                 880 


Leu Gly Phe Ile Lys Gln Phe Gly Gly Thr Ile Ile Glu Leu Gly Phe 
                885                 890                 895     


Gly Lys Ser Val Lys Val Phe Glu Thr Glu Glu Tyr Glu Asp Phe Val 
            900                 905                 910         


Lys Lys His Tyr Ala Asn Asn Arg Ser Lys Arg Asn Lys Leu Leu Leu 
        915                 920                 925             


Glu Asp Ile Pro Glu Lys Met Ile Glu Arg Gln Ile Asn Asp Thr Arg 
    930                 935                 940                 


His Ile Ser Lys Tyr Ile Ser Gly Val Leu Ser Asn Ile Val Arg Ile 
945                 950                 955                 960 


Asp Asp Asp Gln Ala Lys Lys Ile Ile Glu Asp Ala Glu Ile Leu Ala 
                965                 970                 975     


Arg Lys Glu Ile Asp Lys Asp Lys Ala Asn Glu Ile Arg Arg Lys Ala 
            980                 985                 990         


Lys Ser Leu Ala Thr Asp Asp Gly  Ile Asn Ser Lys Asn  Ile Val Pro 
        995                 1000                 1005             


Gly Asn  Gly Lys Ile Thr Thr  Gln Leu Lys Gln Asp  Trp Gly Leu 
    1010                 1015                 1020             


Asn Asp  Val Trp Asn Asp Leu  Ile Leu Pro Arg Phe  Glu Arg Met 
    1025                 1030                 1035             


Asn Gln  Leu Thr Asn Ser Lys  Asp Phe Thr Val Trp  Asn Glu Asn 
    1040                 1045                 1050             


His Gln  Lys Tyr Leu Pro Thr  Val Pro Ile Glu Phe  Ser Lys Gly 
    1055                 1060                 1065             


Phe Ser  Lys Lys Arg Ile Asp  His Arg His His Ala  Leu Asp Ala 
    1070                 1075                 1080             


Leu Val  Ile Ala Cys Ala Thr  Lys Asp His Val Asn  Leu Leu Asn 
    1085                 1090                 1095             


Asn Gln  Ser Ala Lys Ser Asp  Thr Lys Arg Tyr Asp  Leu Lys Lys 
    1100                 1105                 1110             


Lys Leu  Met Lys Phe Glu Lys  Val Val Tyr His His  Thr Gln Thr 
    1115                 1120                 1125             


Gly Asn  Lys Ile Glu Arg Glu  Ile Pro Lys Gln Phe  Leu Lys Pro 
    1130                 1135                 1140             


Trp Glu  Asn Phe Thr Phe Asp  Ala Lys Tyr Asn Leu  Glu Ser Ile 
    1145                 1150                 1155             


Ile Val  Ser Phe Lys Gln Asn  Leu Arg Val Ile Asn  Lys Ala Thr 
    1160                 1165                 1170             


Asn Tyr  Tyr Glu Lys Tyr Val  Glu Lys Asp Gly Thr  Lys Asn Lys 
    1175                 1180                 1185             


Gly Arg  Val Glu Gln Thr Gly  Thr Asn Trp Ala Ile  Arg Lys Pro 
    1190                 1195                 1200             


Met His  Lys Asp Thr Val Ser  Gly Lys Val Asn Leu  Pro Trp Val 
    1205                 1210                 1215             


Lys Val  Pro Lys Gly Lys Ile  Leu Thr Ala Thr Arg  Lys Ile Leu 
    1220                 1225                 1230             


Asp Thr  Ser Phe Asp Leu Lys  Ser Ile Gly Ser Ile  Thr Asp Thr 
    1235                 1240                 1245             


Gly Ile  Gln Lys Ile Leu Arg  Asn Tyr Leu Glu Phe  Lys Gly Ser 
    1250                 1255                 1260             


Pro Glu  Leu Ala Phe Ser Pro  Glu Gly Ile Glu Asp  Leu Asn Lys 
    1265                 1270                 1275             


Asn Ile  Glu Lys Tyr Asn Asp  Gly Lys Pro His Gln  Pro Ile Asn 
    1280                 1285                 1290             


Lys Val  Arg Val Phe Glu Leu  Gly Ser Lys Phe Gln  Val Gly Gln 
    1295                 1300                 1305             


Thr Gly  Asn Lys Lys Asp Lys  Tyr Val Glu Ala Ala  Lys Gly Thr 
    1310                 1315                 1320             


Asn Leu  Phe Phe Ala Val Tyr  Glu Asp Lys Asn Gly  Lys Arg Ser 
    1325                 1330                 1335             


Tyr Glu  Thr Ile Pro Leu Asn  Glu Val Ile Glu Arg  Gln Lys Gln 
    1340                 1345                 1350             


Gly Leu  Ser Val Val Asp Leu  Lys Gly Thr Asn Asp  Phe Tyr Leu 
    1355                 1360                 1365             


Cys Pro  Asn Asp Leu Val Tyr  Ile Pro Ser Asp Asp  Glu Leu Glu 
    1370                 1375                 1380             


Asn Ile  Asn Asn Ile Asp Phe  Lys Asn Ile Ala Lys  Glu Lys Asn 
    1385                 1390                 1395             


Glu Arg  Ile Tyr Lys Val Val  Ser Phe Ser Gly Ser  Gln Ile Phe 
    1400                 1405                 1410             


Phe Val  Arg Gln Asp Ile Ala  Thr Ser Ile Val Asn  Lys Ala Glu 
    1415                 1420                 1425             


Leu Ser  Thr Leu Asn Lys Met  Glu Arg Ala Met Asp  Gly Ser Met 
    1430                 1435                 1440             


Ile Lys  Glu Ser Cys Val Lys  Leu Asn Val Asp Arg  Leu Gly Asn 
    1445                 1450                 1455             


Ile Ser  Lys Ala 
    1460         


<210>  1277
<211>  1466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1277

Met Thr Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Ile Lys Gln Asp Phe Glu Asn Lys Gln Gly Glu Ile Leu 
            20                  25                  30          


Gly Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly Glu 
        35                  40                  45              


Phe Gly Lys Gly Asn Ser Val Ser Gln Thr Ala Ala Arg Thr Asp Tyr 
    50                  55                  60                  


Arg Gly Val Arg Arg Leu Arg Lys Arg Phe Leu Leu Arg Arg Glu Arg 
65                  70                  75                  80  


Leu His Arg Val Leu Asn Val Leu Asn Phe Leu Pro Val His Tyr Ala 
                85                  90                  95      


Ser Gln Ile Asp Phe Glu Lys Arg Phe Gly Lys Phe Lys Glu Asp Thr 
            100                 105                 110         


Glu Pro Lys Leu Ala Tyr Asn Lys Glu Gly Phe Ile Phe Lys Asn Ser 
        115                 120                 125             


Phe Glu Glu Met Val Ala Asp Phe Arg Lys His Gln Pro Gln Leu Leu 
    130                 135                 140                 


Glu Asn Asp Lys Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg 
145                 150                 155                 160 


Lys Lys Ala Leu Ser Lys Lys Ile Glu Lys Glu Glu Leu Ala Trp Ile 
                165                 170                 175     


Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu 
            180                 185                 190         


Glu Glu Glu Glu Asn Pro Asn Lys Leu Val Glu Phe Tyr Ser Leu Lys 
        195                 200                 205             


Ile Thr Asp Val Leu Ala Asp Glu Pro Gln Lys Gly Lys Ser Asp Ile 
    210                 215                 220                 


Trp Tyr Ser Leu Ile Leu Glu Asn Gly Trp Ile Tyr Arg Arg Ser Ser 
225                 230                 235                 240 


Lys Thr Pro Leu Phe Asp Trp Lys Glu Lys Thr Arg Asp Phe Ile Val 
                245                 250                 255     


Thr Thr Asp Leu Asn Asp Asp Gly Ser Ile Lys Thr Asp Lys Asp Gly 
            260                 265                 270         


Asn Glu Lys Arg Ser Phe Arg Ala Pro Ser Glu Asn Asp Trp Thr Leu 
        275                 280                 285             


Val Lys Lys Lys Thr Glu Gln Glu Ile Asp Lys Ser Gln Lys Thr Val 
    290                 295                 300                 


Gly Thr Tyr Ile Tyr Glu Thr Leu Leu Gln Asn Pro Lys Gln Lys Ile 
305                 310                 315                 320 


Lys Gly Lys Leu Val Cys Thr Ile Glu Arg Lys Phe Tyr Lys Glu Glu 
                325                 330                 335     


Leu Lys Gln Ile Leu Glu Lys Gln Lys Glu Phe His Gln Glu Leu Gln 
            340                 345                 350         


Asn Asp Asp Leu Tyr Ser Asp Cys Val His Glu Leu Tyr Arg Thr Asn 
        355                 360                 365             


Glu Ala His Gln Leu Thr Leu Ser Lys Lys Glu Ile Val Asn Leu Phe 
    370                 375                 380                 


Met Glu Asp Leu Ile Phe Tyr Gln Arg Pro Leu Arg Ser Gln Lys Thr 
385                 390                 395                 400 


Ser Ile Ser Asn Cys Thr Leu Glu Phe Arg Lys Tyr Lys Asp Glu Asn 
                405                 410                 415     


Gly Val Glu His Thr Gln Phe Leu Lys Ala Ile Pro Lys Ser Asn Pro 
            420                 425                 430         


Tyr Tyr Gln Glu Phe Arg Ile Trp Gln Trp Ile Phe Asn Leu Asn Ile 
        435                 440                 445             


Tyr Lys Arg Asp Asp Asp Ser Asn Val Thr Lys Glu Phe Leu Asn Thr 
    450                 455                 460                 


Thr Glu Asp Phe Glu Asn Leu Phe Glu Phe Leu Asn Asn Arg Lys Glu 
465                 470                 475                 480 


Val Glu Gln Lys Ala Leu Leu Lys His Phe Lys Leu Asn Glu Lys Leu 
                485                 490                 495     


His Arg Trp Asn Phe Val Glu Asp Lys Lys Tyr Pro Cys Asn Glu Thr 
            500                 505                 510         


Lys Thr Met Ile Ser Thr Arg Leu Asp Lys Val Glu Asn Ile Ser Lys 
        515                 520                 525             


Asp Phe Phe Thr Arg Glu Ile Glu Gln Lys Ile Trp His Ile Ile Tyr 
    530                 535                 540                 


Ser Val Asn Asp Lys Ile Glu Tyr Glu Lys Ala Leu Lys Ser Phe Ala 
545                 550                 555                 560 


Ile Lys His Asn Leu Asp Glu Asn Ser Phe Phe Glu Ala Phe Lys Lys 
                565                 570                 575     


Phe Pro Pro Phe Lys Ser Glu Tyr Gly Ser Phe Ser Glu Lys Ala Ile 
            580                 585                 590         


Lys Lys Leu Leu Pro Leu Met Arg Leu Gly Lys Tyr Trp Asn Glu Asp 
        595                 600                 605             


Glu Ile Leu Lys Tyr Ser Asp Asp Tyr Phe Lys Asn Ile Glu Ser Leu 
    610                 615                 620                 


Leu Gly Val Leu Lys Gln Arg Asp Glu Asn Ile Ser Asp Ala Asp Arg 
625                 630                 635                 640 


Glu Asn Tyr Asn Lys Thr Ile Asn Asn Lys Leu Arg Ala Glu Leu Glu 
                645                 650                 655     


Asn Phe Gln Asn Ala Glu Ile Glu Ser Phe Gln Gly Leu Arg Leu His 
            660                 665                 670         


Ile Ala Gln Tyr Ile Leu Tyr Gly Lys His Ser Glu Gly Asp Asn Lys 
        675                 680                 685             


Lys Phe Asn Ser Ala Asp Asp Leu Glu Lys Phe Leu Lys Glu Phe Lys 
    690                 695                 700                 


Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr 
705                 710                 715                 720 


Leu Arg Val Val Lys Asp Ile Trp Thr Lys Tyr Gly Asn Gly Ala Lys 
                725                 730                 735     


Asp Phe Phe Asp Glu Ile His Ile Glu Leu Gly Arg Asp Met Lys Lys 
            740                 745                 750         


Thr Arg Asp Glu Arg Ala Asp Ala Thr Lys Ile Ile Thr Glu Asn Glu 
        755                 760                 765             


Asn Thr Asn Leu Arg Ile Lys Ala Leu Leu Ala Glu Met Met Asn Asp 
    770                 775                 780                 


Val Glu Met Glu Asn Val Arg Pro Tyr Ser Pro Met Gln Gln Glu Ile 
785                 790                 795                 800 


Leu Lys Ile Tyr Glu Asp Gly Ile Leu Lys Ser Asp Ile Glu Ile Glu 
                805                 810                 815     


Asp Asp Ile Leu Lys Ile Ser Lys Thr Ala Gln Pro Ser Ser Ser Asp 
            820                 825                 830         


Leu Lys Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Lys Ser Pro Tyr 
        835                 840                 845             


Thr Gly Gln Ile Ile Pro Leu Asn Lys Leu Phe Thr Pro Glu Tyr Glu 
    850                 855                 860                 


Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Ser 
865                 870                 875                 880 


Asn Lys Ile Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp Asn Tyr 
                885                 890                 895     


Ile Gly Leu Gly Phe Ile Lys Gln Phe Gly Gly Thr Ile Val Glu Cys 
            900                 905                 910         


Gly Phe Gly Lys Asn Val Lys Val Phe Glu Ala Glu Glu Tyr Glu Asp 
        915                 920                 925             


Phe Val Lys Lys His Tyr Ala Asn Asn Arg Gly Lys Arg Asn Lys Leu 
    930                 935                 940                 


Leu Leu Glu Glu Ile Pro Glu Lys Met Ile Glu Arg Gln Met Asn Asp 
945                 950                 955                 960 


Thr Arg His Ile Ser Lys Tyr Ile Ser Gly Val Leu Ser Asn Ile Val 
                965                 970                 975     


Arg Val Glu Asp Gly Ser Asp Glu Gly Val Asn Ser Lys Asn Ile Val 
            980                 985                 990         


Pro Gly Asn Gly Lys Ile Thr Thr  Gln Leu Lys Gln Asp  Trp Gly Leu 
        995                 1000                 1005             


Asn Asp  Val Trp Asn Glu Leu  Ile Leu Pro Arg Phe  Glu Arg Met 
    1010                 1015                 1020             


Asn Gln  Leu Thr Asn Ser Thr  Asp Phe Thr Ala Trp  Asn Glu Asn 
    1025                 1030                 1035             


His Gln  Lys Phe Leu Pro Thr  Val Pro Ile Glu Phe  Ser Lys Gly 
    1040                 1045                 1050             


Phe Ser  Lys Lys Arg Ile Asp  His Arg His His Ala  Leu Asp Ala 
    1055                 1060                 1065             


Leu Ile  Ile Ala Cys Ala Thr  Lys Asp His Val Asn  Leu Leu Asn 
    1070                 1075                 1080             


Asn Gln  Ser Ala Lys Ser Asp  Thr Lys Arg Tyr Asp  Leu Lys Lys 
    1085                 1090                 1095             


Lys Leu  Met Lys Phe Glu Lys  Val Val Tyr Asn His  Pro Gln Thr 
    1100                 1105                 1110             


Gly Asp  Arg Ile Glu Arg Glu  Val Pro Lys Gln Phe  Leu Lys Pro 
    1115                 1120                 1125             


Trp Asp  Asn Phe Thr Phe Glu  Ala Lys Asn Asn Leu  Asp Lys Ile 
    1130                 1135                 1140             


Ile Val  Ser Phe Lys Gln Asn  Leu Arg Val Ile Asn  Lys Ala Thr 
    1145                 1150                 1155             


Asn Tyr  Tyr Glu Lys Phe Val  Glu Lys Asp Gly Val  Lys Thr Lys 
    1160                 1165                 1170             


Glu Arg  Val Glu Gln Lys Gly  Thr Asn Trp Ala Ile  Arg Lys Ser 
    1175                 1180                 1185             


Leu His  Glu Glu Thr Val Ser  Gly Lys Ile Asp Leu  Pro Trp Val 
    1190                 1195                 1200             


Glu Thr  Asn Asn Gly Glu Phe  Ile Thr Ala Thr Arg  Lys Ser Leu 
    1205                 1210                 1215             


Asp Ala  Ser Phe Asn Leu Glu  Lys Ile Lys Lys Ile  Thr Asp Thr 
    1220                 1225                 1230             


Gly Ile  Gln Lys Ile Leu Arg  Asn Tyr Leu Lys Ala  Lys Asp Asp 
    1235                 1240                 1245             


Asn Ala  Glu Leu Ala Phe Ser  Pro Glu Gly Ile Glu  Asp Leu Asn 
    1250                 1255                 1260             


Lys Asn  Ile Glu Lys Tyr Asn  Asp Gly Lys Ser His  Gln Pro Ile 
    1265                 1270                 1275             


Val Arg  Val Arg Val Tyr Glu  Lys Gly Lys Gly Arg  Phe Ala Leu 
    1280                 1285                 1290             


Gly Gln  Thr Gly Asn Asn Ile  Asp Lys Tyr Val Gln  Gly Ser Pro 
    1295                 1300                 1305             


Asn Leu  Phe Phe Ala Ile Tyr  Lys Asp Glu Asn Gly  Lys Lys Ser 
    1310                 1315                 1320             


Tyr Glu  Ser Ile Arg Leu Asp  Ile Val Ile Glu Arg  Met Lys Gln 
    1325                 1330                 1335             


Gly Leu  Gln Ala Val Pro Glu  Thr Asn Pro Asn Gly  Ile Ser Leu 
    1340                 1345                 1350             


Tyr Gln  Asn Leu Ser Pro Leu  Asp Leu Val Tyr Ile  Pro Thr Glu 
    1355                 1360                 1365             


Asp Glu  Ile Glu Ser Pro His  Leu Ile Asp Phe Thr  Ile Leu Asn 
    1370                 1375                 1380             


Lys Thr  Arg Leu Glu Arg Ile  Tyr Asn Val Asn Asp  Phe Ser Gly 
    1385                 1390                 1395             


Tyr Thr  Ile Tyr Phe Ser Gln  Asn Ser Phe Ala Lys  Asn Ile Tyr 
    1400                 1405                 1410             


Pro Lys  Glu Met Asp Met Thr  Trp Asn Glu Lys Lys  Gln Lys Pro 
    1415                 1420                 1425             


Ser Gly  Ser Phe Asp Ser Lys  Thr Ala Ser Tyr Asn  Lys Lys Ser 
    1430                 1435                 1440             


Ile Lys  Asp Ile Phe Ile Lys  Leu Lys Ile Asp Arg  Leu Gly Asn 
    1445                 1450                 1455             


Ile Ser  Lys Val Ser Asn Thr  Leu 
    1460                 1465     


<210>  1278
<211>  1466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1278

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Glu Asp Phe Glu Asn Lys Gln Gly Glu Ile Leu Gly 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Glu Phe 
        35                  40                  45              


Gly Arg Gly Asn Ser Ile Ser Gln Thr Ala Asp Arg Thr Gly Tyr Arg 
    50                  55                  60                  


Gly Val Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Asn Ile Leu Gly Phe Leu Pro Asn His Tyr Ala Lys 
                85                  90                  95      


Glu Ile Asp Phe Thr Lys Arg Leu Gly Lys Phe Ile Asn His Ala Glu 
            100                 105                 110         


Pro Lys Leu Ser Phe Asp Lys Glu Phe Ile Phe Lys Asp Ser Phe Asn 
        115                 120                 125             


Glu Met Leu Lys Asp Phe Gln Gln Asn Gln Pro Gly Phe Ile Ile Asn 
    130                 135                 140                 


Lys His Gly Glu Glu Ala Leu Val Pro Tyr Asp Trp Thr Ile Tyr Tyr 
145                 150                 155                 160 


Leu Arg Lys Lys Ala Leu Thr Gln Lys Ile Asp Lys Glu Glu Leu Ala 
                165                 170                 175     


Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg 
            180                 185                 190         


Gly Glu Glu Glu Glu Glu Asn Thr Asn Lys Leu Val Glu Phe Tyr Ser 
        195                 200                 205             


Leu Lys Ile Ile Asp Val Ile Ala Asp Asn Glu Val Asn Lys Lys Gly 
    210                 215                 220                 


Glu Val Trp Tyr Ser Leu His Leu Glu Asn Gly Trp Ile Tyr Arg Arg 
225                 230                 235                 240 


Ser Ser Lys Ile Ser Leu Val Asp Trp Lys Asp Lys Val Arg Asp Phe 
                245                 250                 255     


Ile Val Thr Thr Asp Val Asn Asp Asp Gly Ser Glu Lys Leu Asp Lys 
            260                 265                 270         


Asp Gly Val Val Lys Arg Ser Phe Arg Ala Pro Ser Ala Asp Asp Trp 
        275                 280                 285             


Thr Leu Leu Lys Lys Lys Thr Glu Gln Asp Ile Asp Asn Ser Asn Lys 
    290                 295                 300                 


Thr Val Gly Ala Tyr Ile Tyr Glu Asn Leu Leu Leu Asn Pro Lys Gln 
305                 310                 315                 320 


Lys Ile Lys Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys 
                325                 330                 335     


Asp Glu Leu Lys Gln Ile Leu Glu Lys Gln Lys Glu Phe His Gln Glu 
            340                 345                 350         


Leu Gln Asn Glu Gln Leu Leu Gln Asp Cys Val Arg Glu Leu Tyr Lys 
        355                 360                 365             


Tyr Asn Glu Gln His Gln Gln Met Leu Glu Ser Lys Asp Phe Val His 
    370                 375                 380                 


Leu Phe Leu Asn Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser Gln 
385                 390                 395                 400 


Lys Ser Leu Ile Ser Asn Cys Thr Leu Glu Lys Arg Val Ser Arg Asp 
                405                 410                 415     


Gly Val Val Phe Pro Ile Lys Val Ile Ser Lys Ser Asn Pro Tyr Tyr 
            420                 425                 430         


Gln Glu Phe Arg Leu Leu Gln Trp Leu Gln Asn Leu Ala Ile Tyr Arg 
        435                 440                 445             


Lys Asp Asp Asp Val Asn Val Thr Gln Glu Phe Leu Asn Ser Ile Glu 
    450                 455                 460                 


Ser Trp Glu Asn Leu Leu Gln Phe Leu Asn Ala Lys Lys Glu Ile Lys 
465                 470                 475                 480 


Gln Glu Gln Leu Ile Lys Phe Leu Leu Glu Gln Lys Gly Leu Lys Gly 
                485                 490                 495     


Asn Ser Leu Lys Thr Ala Ile Leu Thr Tyr Arg Trp Asn Tyr Val Thr 
            500                 505                 510         


Asp Lys Val Tyr Pro Met Asn Glu Thr Arg Ser Leu Ile Gln Asp Lys 
        515                 520                 525             


Leu Lys Lys Val Glu Asn Val Ala Thr Asp Phe Leu Ser Phe Asp Asn 
    530                 535                 540                 


Glu Met Ala Leu Trp His Ile Ile Tyr Ser Val Asn Asp Lys Ile Glu 
545                 550                 555                 560 


Tyr Glu Lys Ala Leu Ala Lys Phe Ala Ser Lys His Asn Leu Asp Glu 
                565                 570                 575     


Ile Ser Phe Val Glu Ser Phe Lys Lys Phe Pro Pro Phe Lys Ser Asp 
            580                 585                 590         


Tyr Gly Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu Met 
        595                 600                 605             


Arg Phe Gly Ser Val Trp Asn Lys Asn Asn Ile Val Pro Ser Val Leu 
    610                 615                 620                 


Asp Arg Ile Asp Lys Ile Leu Thr Gly Glu Tyr Asp Glu Lys Ile Lys 
625                 630                 635                 640 


Ile Arg Val Arg Glu Lys Ala Glu Gln Phe Gln Leu Asn Gln Ile Glu 
                645                 650                 655     


Asp Phe Gln Asn Leu Pro Leu Trp Leu Ala Gln Tyr Val Val Tyr Asp 
            660                 665                 670         


Arg His Ser Glu Ala Glu Ile Ala Gly Lys Trp Asn Ser Val Lys Asp 
        675                 680                 685             


Leu Glu Asn Tyr Leu Lys Glu Phe Lys Gln His Ser Leu Arg Asn Pro 
    690                 695                 700                 


Ile Val Glu Gln Ile Ile Thr Glu Thr Leu Arg Val Val Lys Asp Ile 
705                 710                 715                 720 


Trp Gln Gln Tyr Gly Asn Gly Val Gln Asn Phe Phe Asp Glu Ile His 
                725                 730                 735     


Ile Glu Leu Gly Arg Glu Met Lys Asn Thr Ala Asp Glu Arg Lys Arg 
            740                 745                 750         


Ile Thr Asn Ser Val Thr Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys 
        755                 760                 765             


Ala Leu Leu Val Glu Leu Lys Glu Asp Thr Asn Ile Glu Asn Val Arg 
    770                 775                 780                 


Pro His Ser Pro Met Gln Gln Glu Ile Leu Lys Ile Tyr Glu Glu Gly 
785                 790                 795                 800 


Val Leu Asn Ala Val Glu Asn Ile Asp Glu Asp Ile Leu Lys Ile Ser 
                805                 810                 815     


Lys Thr Ala Gln Pro Ser Lys Ser Glu Leu Ile Arg Tyr Lys Leu Trp 
            820                 825                 830         


Leu Glu Gln Lys Tyr Gln Ser Pro Tyr Thr Gly Glu Met Ile Pro Leu 
        835                 840                 845             


Ser Arg Leu Phe Thr Ser Asp Tyr Glu Ile Glu His Val Ile Pro Gln 
    850                 855                 860                 


Ser Arg Tyr Phe Asp Asp Ser Leu Ser Asn Lys Val Ile Cys Glu Ala 
865                 870                 875                 880 


Ala Val Asn Lys Arg Lys Asp Asn Gln Leu Gly Leu Glu Phe Ile Lys 
                885                 890                 895     


Asn His His Ser Glu Lys Ile Glu Leu Gly Asn Gly Arg Thr Ala Gln 
            900                 905                 910         


Val Leu Glu Val Glu Ala Tyr Glu Ser Phe Val Lys Lys Asn Tyr Asp 
        915                 920                 925             


Lys Asn Arg Gly Lys Arg Asn Lys Leu Leu Leu Glu Asp Val Pro Glu 
    930                 935                 940                 


Lys Met Ile Glu Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser Lys Phe 
945                 950                 955                 960 


Val Thr His Leu Leu Ser Asn Ile Val Arg Lys Asp Glu Val Asn Ser 
                965                 970                 975     


Lys Asp Asp Gly Ile Asn Ser Lys Asn Ile Ile Pro Gly Asn Gly Lys 
            980                 985                 990         


Ile Thr Ser Gln Leu Lys Gln Asp  Trp Gly Leu Asn Asp  Val Trp Asn 
        995                 1000                 1005             


Glu Leu  Ile Leu Pro Arg Phe  Glu Arg Met Asn Asn  Leu Thr Asn 
    1010                 1015                 1020             


Ser Thr  Asp Phe Thr Ser Thr  Asn Thr Asn Gly Lys  Ile Ile Pro 
    1025                 1030                 1035             


Thr Val  Pro Leu Glu Tyr Ser  Lys Gly Phe Ser Lys  Lys Arg Ile 
    1040                 1045                 1050             


Asp His  Arg His His Ala Leu  Asp Ala Leu Val Ile  Ala Leu Ala 
    1055                 1060                 1065             


Thr Arg  Asp His Val Asn Leu  Leu Asn Asn Glu Ser  Ala Leu Pro 
    1070                 1075                 1080             


Lys Lys  Asp Arg Thr Lys Glu  Glu Gln Lys Ser Phe  Arg Phe Asp 
    1085                 1090                 1095             


Leu Gln  Val Asn Leu Arg Asn  Lys Glu Lys Tyr Phe  Asn Val Lys 
    1100                 1105                 1110             


Glu Gly  Arg Glu Lys Glu Lys  Phe Thr Thr Phe Lys  Lys Pro Trp 
    1115                 1120                 1125             


Asn Thr  Leu Thr Gln Asp Thr  Arg Asp Ala Leu Glu  Asn Met Val 
    1130                 1135                 1140             


Val Ser  Phe Lys Gln Asn Leu  Arg Val Ile Asn Lys  Ala Thr Asn 
    1145                 1150                 1155             


Tyr Tyr  Glu Ser Tyr Thr Asp  Glu Asn Gly Asn Leu  Arg Leu Asp 
    1160                 1165                 1170             


Lys Tyr  Gly Asn Pro Lys Lys  Glu Leu Ile Ser Gln  Lys Gly Thr 
    1175                 1180                 1185             


Asn Trp  Ala Ile Arg Lys Ser  Leu His Lys Glu Thr  Val Ser Gly 
    1190                 1195                 1200             


Gln Ile  His Leu Asp Arg Val  Lys Val Thr Lys Gly  Lys Ile Val 
    1205                 1210                 1215             


Thr Ala  Thr Arg Lys Ser Leu  Asp Ser Ser Phe Asn  Glu Lys Ser 
    1220                 1225                 1230             


Ile Glu  Ser Ile Thr Asp Thr  Gly Ile Gln Lys Ile  Leu Arg Asn 
    1235                 1240                 1245             


Tyr Leu  Lys Ala Lys Asp Asn  Lys Pro Asp Val Ala  Phe Ser Pro 
    1250                 1255                 1260             


Glu Gly  Ile Glu Glu Leu Asn  Lys Ser Ile Ser Val  Tyr Asn Glu 
    1265                 1270                 1275             


Gly Lys  Asn His Gln Pro Ile  Ile Lys Val Arg Ile  Phe Glu Gln 
    1280                 1285                 1290             


Gly Ser  Lys Phe Pro Val Gly  Glu Thr Gly Asn Lys  Ile Ala Lys 
    1295                 1300                 1305             


Phe Val  Glu Ala Ala Lys Gly  Thr Asn Leu Phe Phe  Gly Ile Tyr 
    1310                 1315                 1320             


Gln Asp  Lys Thr Gly Lys Arg  Ser Tyr Asp Thr Ile  Pro Leu Asn 
    1325                 1330                 1335             


Leu Val  Ile Glu Arg Leu Lys  Gln Gly Leu Ser Pro  Val Pro Glu 
    1340                 1345                 1350             


Thr Asn  Glu Lys Gly His Gln  Leu Leu Phe His Leu  Ser Pro Asn 
    1355                 1360                 1365             


Asp Leu  Val Tyr Val Pro Thr  Ile Asp Asp Asn Glu  Gln Leu Asp 
    1370                 1375                 1380             


Ser Asn  Arg Val Tyr Lys Phe  Thr Asp Ser Ser Asp  Thr Thr Cys 
    1385                 1390                 1395             


Asn Phe  Ile Pro Ser Asn Ile  Ser Ala Ile Ile Phe  Asn Ile Asn 
    1400                 1405                 1410             


Ser Lys  Glu Gln Glu Lys Arg  Gly Ile Asn Tyr Pro  Ile Gln Asn 
    1415                 1420                 1425             


Glu Leu  Gly Val Gly Ser Pro  Gln Ser Lys Asn Gln  Lys Ser Phe 
    1430                 1435                 1440             


Asp Gly  Phe Met Ile Lys Glu  Val Cys Ile Lys Leu  Lys Val Asp 
    1445                 1450                 1455             


Arg Leu  Gly Asn Ile Ser Lys  Thr 
    1460                 1465     


<210>  1279
<211>  1471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1279

Met Cys Lys Tyr Phe Tyr Ile Lys Asn Phe Ile Thr Phe Val Leu Ser 
1               5                   10                  15      


Lys Ser Ile Ile Pro Met Lys His Ile Leu Gly Leu Asp Leu Gly Ser 
            20                  25                  30          


Asn Ser Ile Gly Trp Ala Phe Val Gln Gln Asp Phe Glu Asn Lys Gln 
        35                  40                  45              


Gly Lys Ile Ile Ala Thr Gly Ser Arg Ile Ile Pro Met Asp Gln Gly 
    50                  55                  60                  


Ile Leu Gly Asp Phe Glu Arg Gly Asn Thr Val Ser Gln Thr Ala Glu 
65                  70                  75                  80  


Arg Thr Thr Tyr Arg Ser Thr Arg Arg Leu Arg Glu Arg His Leu Leu 
                85                  90                  95      


Arg Arg Glu Arg Leu His Arg Val Leu His Ile Leu Gly Phe Leu Pro 
            100                 105                 110         


Pro His Tyr Asp Ala Gln Ile Asp Phe Thr Lys Arg Tyr Gly Lys Phe 
        115                 120                 125             


Ile Asp Asn Ala Glu Pro Lys Ile Ala Tyr Asn Asn Gly Asn Phe Ile 
    130                 135                 140                 


Phe Met Asn Ser Phe Asn Glu Met Ile Glu Asp Phe Lys Lys His Gln 
145                 150                 155                 160 


Pro Gln Leu Phe Tyr Lys Lys Ser Asn Gly Glu Glu Ser Lys Ile Pro 
                165                 170                 175     


Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Lys 
            180                 185                 190         


Ile Thr Gln Gln Glu Leu Ala Trp Leu Ile Leu His Phe Asn Gln Lys 
        195                 200                 205             


Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Thr Glu Asn Pro Asn 
    210                 215                 220                 


Lys Glu Val Ala Phe His Ser Leu Lys Val Val Asp Val Glu Ala Glu 
225                 230                 235                 240 


Ala Pro Asn Lys Lys Gly Glu Ile Trp Tyr Thr Ile Arg Leu Glu Asn 
                245                 250                 255     


Gly Trp Ile Tyr Arg Arg Thr Ser Lys Ile Pro Leu Asp Asp Trp Lys 
            260                 265                 270         


Gly Lys Thr Arg Asp Phe Ile Val Thr Thr Asp Leu Asn Asp Asp Gly 
        275                 280                 285             


Thr Ile Lys Val Asp Lys Glu Gly Lys Glu Lys Arg Ser Phe Arg Ala 
    290                 295                 300                 


Pro Asp Glu Asn Asp Trp Asn Leu Leu Lys Lys Lys Thr Glu His Asp 
305                 310                 315                 320 


Ile Asp Phe Ser Gly Lys Thr Val Gly Ala Tyr Ile Tyr Asp His Leu 
                325                 330                 335     


Leu Gln Asn Pro Asn Gln Lys Ile Lys Gly Lys Leu Val Arg Thr Ile 
            340                 345                 350         


Glu Arg Lys Tyr Tyr Lys Asp Glu Leu Lys Ala Ile Leu Ala Lys Gln 
        355                 360                 365             


Ile Ala Leu Gln Pro Glu Leu Phe Thr Glu Thr Leu Leu Ala Asp Cys 
    370                 375                 380                 


Ile Arg Glu Leu Tyr Arg Lys Asn Glu Thr Gln Gln Gln Asn Leu Leu 
385                 390                 395                 400 


Ser Lys Asp Phe Leu His Leu Phe Val Glu Asp Ile Ile Phe Tyr Gln 
                405                 410                 415     


Arg Pro Leu Arg Ser Lys Lys Ser Thr Ile Ala Asn Cys Thr Leu Glu 
            420                 425                 430         


Lys Arg Ser Tyr Ile Asp Lys Glu Thr Asn Glu Arg Lys Asp Ala Pro 
        435                 440                 445             


Ile Lys Val Cys Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Val 
    450                 455                 460                 


Leu Gln Trp Leu Gln Asn Leu Arg Ile Tyr Glu Ile Glu Thr Asp Arg 
465                 470                 475                 480 


Glu Val Thr Ala Glu Phe Ile Glu Thr Pro Gln Asp Cys Glu Ala Leu 
                485                 490                 495     


Phe Asn Phe Leu Met Ser Gln Lys Glu Ile Asp Asn Lys Gly Leu Leu 
            500                 505                 510         


Lys Tyr Phe Leu Phe Ala Lys Asn Pro Gln Ile Lys Asp Lys Ala Ala 
        515                 520                 525             


Lys Ser Glu Leu Lys Lys Trp Leu Thr Thr Tyr Arg Trp Asn Tyr Val 
    530                 535                 540                 


Tyr Asp Ala Ala Asp Asn Ser Ser Lys Lys Tyr Pro Met Asn Glu Thr 
545                 550                 555                 560 


Gly Tyr Glu Leu Gln Arg Tyr Leu Gln Lys Val Glu Gly Leu Pro Ala 
                565                 570                 575     


Asn Phe Leu Thr Phe Asp Ile Thr Tyr Gln Leu Trp His Leu Leu Tyr 
            580                 585                 590         


Ser Val Lys Asp Lys Val Glu Phe Glu Lys Ala Leu Lys Lys Phe Ala 
        595                 600                 605             


Lys Lys Lys Gln Leu Asp Lys Thr Ser Phe Val Asp Ser Phe Lys Asn 
    610                 615                 620                 


Phe Lys Pro Tyr Pro Asn Glu Tyr Gly Ser Phe Ser Glu Lys Ala Ile 
625                 630                 635                 640 


Lys Lys Leu Leu Pro Leu Met Arg Phe Gly Asn His Trp Asp Phe Asn 
                645                 650                 655     


Arg Ile Asp Val Ser Thr Gln Lys Arg Ile Asp Asp Leu Ile Asn Gly 
            660                 665                 670         


Val Ala Asn Glu Asp Ile Arg Thr Ile Ile Arg Glu Lys Ala Glu Lys 
        675                 680                 685             


Tyr Gln Leu Asn Ser Glu Thr Cys Phe Gln Asp Leu Pro Leu Trp Leu 
    690                 695                 700                 


Ala Gln Tyr Val Val Tyr Asn Arg His Ala Glu Ala Thr Asp Ile Glu 
705                 710                 715                 720 


Lys Trp Thr Ser Val Ser Asp Leu Glu Ala Tyr Leu Glu Asp Phe Lys 
                725                 730                 735     


Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val Thr Glu Thr 
            740                 745                 750         


Leu Arg Val Val Arg Asp Ile Trp Gln Gln Tyr Gly Gly Gly Gln Ala 
        755                 760                 765             


Asn Phe Phe Asp Glu Ile His Ile Glu Leu Gly Arg Glu Leu Lys Lys 
    770                 775                 780                 


Thr Ala Glu Glu Arg Lys Gln Leu Ser Asp Lys Asn Gln Glu Asn Glu 
785                 790                 795                 800 


Ser Thr Asn Leu Arg Ile Lys Lys Leu Leu Ala Glu Leu Lys Glu Asp 
                805                 810                 815     


Ala Ser Ile Gln Asn Val Arg Pro Tyr Ser Leu Val Gln Gln Glu Leu 
            820                 825                 830         


Leu Lys Ile Tyr Glu Glu Gly Val Phe Ser Ala Thr Asp Glu Val Glu 
        835                 840                 845             


Glu Asp Ile Gln Lys Ile Arg Lys Lys Ala Glu Pro Ser Lys Ala Asp 
    850                 855                 860                 


Leu Gln Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Lys Ser Pro Tyr 
865                 870                 875                 880 


Thr Gly Glu Val Ile Ser Leu Thr Lys Leu Phe Thr Glu Glu Tyr Glu 
                885                 890                 895     


Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Tyr Asp Asp Ser Leu Ser 
            900                 905                 910         


Asn Lys Val Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Thr Asn Gln 
        915                 920                 925             


Leu Gly Leu Glu Phe Ile Gln Asn His His Gly Glu Lys Val Arg Ile 
    930                 935                 940                 


Thr Ala Asn Lys Glu Val Thr Ile Phe Thr Glu Glu Gln Tyr Lys Glu 
945                 950                 955                 960 


Phe Val Lys Lys His Tyr Ala Asn Asn Ser Ala Lys Gln Gln Lys Leu 
                965                 970                 975     


Leu Leu Asn Glu Ile Pro Glu Lys Met Val Ala Arg Gln Leu Asn Asp 
            980                 985                 990         


Thr Arg Tyr Ile Ser Lys Tyr Ile  Ser Glu Ile Leu Ser  Lys Ile Val 
        995                 1000                 1005             


Arg Ser  Asp Glu Lys Asp Glu  Gly Val Asn Ser Lys  Asn Ile Ile 
    1010                 1015                 1020             


Pro Cys  Thr Gly Lys Ile Thr  Thr Ala Leu Lys Gln  Asp Trp Gly 
    1025                 1030                 1035             


Leu Asn  Asp Val Trp Asn Lys  Leu Ile Leu Pro Arg  Phe Glu Arg 
    1040                 1045                 1050             


Met Asn  Gln Leu Thr Gln Thr  Gln Leu Phe Thr Ser  Tyr Asn Asp 
    1055                 1060                 1065             


Arg Leu  Gln Lys Asn Leu Pro  Thr Val Pro Ile Glu  Tyr Ser Gln 
    1070                 1075                 1080             


Gly Phe  Gln Lys Lys Arg Ile  Asp His Arg His His  Ala Met Asp 
    1085                 1090                 1095             


Ala Leu  Val Ile Ala Cys Ala  Thr Arg Glu His Ile  Asn Tyr Met 
    1100                 1105                 1110             


Asn Asn  Gln His Ala Leu Asp  Lys Ser Lys Asp Lys  Lys Glu Lys 
    1115                 1120                 1125             


Gln Gln  Ser Arg Ile Asp Leu  Arg Thr Ile Leu Cys  Glu Lys Lys 
    1130                 1135                 1140             


Asn Asn  Asn Trp Gln Phe Lys  Lys Pro Trp Ser Thr  Phe Thr Gln 
    1145                 1150                 1155             


Glu Ala  Lys Glu Ala Leu Glu  His Ile Val Val Ser  Phe Lys Gln 
    1160                 1165                 1170             


Asn Leu  Arg Val Ile Asn Lys  Ala Thr Asn Tyr Tyr  Glu Lys Trp 
    1175                 1180                 1185             


Glu Glu  Lys Asn Gly Val Leu  Val Lys Lys Lys Val  Lys Gln Glu 
    1190                 1195                 1200             


Gly Thr  Asn Trp Ala Ile Arg  Lys Pro Leu His Lys  Glu Thr Val 
    1205                 1210                 1215             


Ser Gly  Lys Ile Val Leu Asp  Arg Lys Ile Gly Lys  Asp Lys Ile 
    1220                 1225                 1230             


Leu Thr  Ala Thr Arg Lys Ala  Val Asp Ile Thr Phe  Thr Glu Lys 
    1235                 1240                 1245             


Ile Ile  Ser Ser Ile Thr Asp  Thr Gly Ile Gln Lys  Ile Leu Leu 
    1250                 1255                 1260             


Asn Tyr  Leu Lys Tyr Lys Gly  Ser Pro Glu Val Ala  Phe Ser Thr 
    1265                 1270                 1275             


Glu Gly  Leu Glu Glu Leu Asn  Lys Asn Ile Ala Gln  Tyr Asn Asn 
    1280                 1285                 1290             


Gly Lys  Lys His Gln Pro Ile  Tyr Lys Val Arg Ile  Phe Glu Glu 
    1295                 1300                 1305             


Gly Ser  Lys Phe Pro Leu Gly  Glu Thr Gly Ala Lys  Ala Thr Lys 
    1310                 1315                 1320             


Tyr Val  Glu Ala Ala Lys Gly  Thr Asn Leu Phe Phe  Gly Val Tyr 
    1325                 1330                 1335             


Gln Gly  Lys Gly Lys Arg Thr  Tyr Ala Thr Ile Pro  Leu Asn Glu 
    1340                 1345                 1350             


Val Ile  Glu Arg Gln Lys Gln  Gly Leu Pro Ser Val  Pro Glu Arg 
    1355                 1360                 1365             


Asn Glu  Lys Gly Glu Pro Leu  Leu Phe Ser Leu Ser  Pro Asn Asp 
    1370                 1375                 1380             


Leu Val  Tyr Val Pro Met Glu  Gly Glu Ile Ala Glu  Thr Ile Asp 
    1385                 1390                 1395             


Phe Asn  Asn Leu Ser Lys Glu  Gln Lys Glu Arg Ile  Tyr Lys Thr 
    1400                 1405                 1410             


Val Ser  Phe Thr Gly Asn Gln  Cys Phe Phe Val Gln  Glu Ala Val 
    1415                 1420                 1425             


Ala Thr  Pro Ile Val Asn Lys  Met Glu Tyr Ser Pro  Leu Asn Lys 
    1430                 1435                 1440             


Met Glu  Arg Asp Ile Leu Gly  Thr Met Ile Lys Glu  Val Cys Ile 
    1445                 1450                 1455             


Lys Leu  Lys Val Asp Arg Leu  Gly Asn Ile Thr Lys  Ala 
    1460                 1465                 1470     


<210>  1280
<211>  1471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1280

Met Gly Tyr Lys Ala Pro Arg Asn Asp Lys Gly Glu Ile Asp Tyr Gly 
1               5                   10                  15      


Val Gly Leu Asp Leu Gly Asn Gly Ser Val Gly Trp Val Ala Met Asp 
            20                  25                  30          


Lys Asp Tyr His Leu Ile Arg Ala Arg Gly His Glu Leu Ile Gly Ala 
        35                  40                  45              


Arg Leu Phe Asp Pro Ala Asn Ser Ala Glu Asn Arg Arg Glu Ser Arg 
    50                  55                  60                  


Thr Thr Arg Arg Arg Leu Ser Arg Arg Arg Trp Arg Leu Arg Met Leu 
65                  70                  75                  80  


Asp Ser Leu Phe Ala Ser Glu Leu Ala Lys Ile Asp Pro Asn Phe Leu 
                85                  90                  95      


Ala Arg Arg Lys Tyr Ser Trp Val His Pro Lys Asp Glu Gln Asn His 
            100                 105                 110         


Glu Asn Trp Tyr Gly Ser Ile Ile Phe Gly Ser Lys Glu Gln Asp Lys 
        115                 120                 125             


Gln Phe Tyr Lys Asp Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu 
    130                 135                 140                 


Thr Glu Asp Thr Glu Arg His Asp Ile Arg Glu Ile Tyr Val Ala Ile 
145                 150                 155                 160 


His His Ile Val Lys Tyr Arg Gly His Phe Leu Arg Glu Gly Glu Ile 
                165                 170                 175     


Asn Ser Glu Asn Leu Phe Asp Pro Glu Gly Phe Val Glu Leu Ile Lys 
            180                 185                 190         


Asp Val Ile Lys Leu Asn Asn Ser Tyr Asp Glu Ser Thr Pro Ile Phe 
        195                 200                 205             


Glu Ala Ser Pro Ala Asp Val Ser Glu Ala Leu Leu Asn Glu His Thr 
    210                 215                 220                 


Ser Arg Ser Gly Arg Ile Glu Asp Thr Leu Asp Lys Leu Asp Phe Ser 
225                 230                 235                 240 


Val Ser Arg Val Asp Lys His Glu Leu Lys Gly Met Arg Asp Ala Ala 
                245                 250                 255     


Lys Ala Val Ile Gly Ala Ile Val Gly Asn Thr Ala Asp Phe Ser Lys 
            260                 265                 270         


Ile Phe Asn Leu Asp Asn Leu Asp Lys Asp Ser Lys Asp Asn Leu Lys 
        275                 280                 285             


Leu Lys Phe Ala Asp Ser Asn Phe Asp Glu Lys Tyr Gly Gly Val Val 
    290                 295                 300                 


Gly Ser Gly Leu Leu Asp Glu Thr Gln Ile Glu Leu Val Asp Arg Ile 
305                 310                 315                 320 


His Ala Val Tyr Asp Ala Val Ala Leu Lys Gly Ile Leu Gly Asp Ala 
                325                 330                 335     


His Thr Ile Ser Glu Ala Lys Val Glu Ser Tyr Glu Lys His Arg Lys 
            340                 345                 350         


Asn Trp Lys Leu Ile Lys Glu Glu Leu Arg Thr Lys Glu Asn Lys Asp 
        355                 360                 365             


Glu Val Asp Leu Trp Tyr Gly Asp Leu Ser Gly Arg Gln Tyr Lys Asn 
    370                 375                 380                 


Pro Pro Asp Pro Glu Lys Gly Pro Asp Glu Gly Ser Lys Lys Asp Ser 
385                 390                 395                 400 


Pro Ile Arg Asp Ser Asp Gln Lys Gln Asn Leu Ile Lys Lys Ala Asn 
                405                 410                 415     


Asp Tyr Phe Thr Glu Leu Ile Asp Asp Ser Gln Leu Ser Glu Glu Gln 
            420                 425                 430         


Lys Asn Cys Leu Lys Asn Asp Ile Asn Asn Gly Gln Leu Phe Pro Val 
        435                 440                 445             


Gln Arg Ser Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu Asn 
    450                 455                 460                 


Glu Leu Arg Arg Ile Ile Glu Asn Gln Lys Gln Tyr Tyr Pro Phe Leu 
465                 470                 475                 480 


Ala Glu Thr Phe Ile Arg Lys Thr Ala Glu Gly Lys Ser Tyr Glu Glu 
                485                 490                 495     


Asn Lys Ile Val Ala Leu Leu Lys Phe Arg Val Pro Tyr Tyr Val Gly 
            500                 505                 510         


Pro Leu Val Ser Lys Glu Asp Met Glu Glu His Ser Ser Gly Asn Ser 
        515                 520                 525             


Ala Tyr His Trp Met Ala Arg Asn Pro Gly Arg Gly Glu Ala Ile Thr 
    530                 535                 540                 


Pro Trp Asn Phe Asp Glu Val Val Asp Lys Asp Glu Ser Gly Ala Arg 
545                 550                 555                 560 


Phe Ile Ser Arg Leu Thr Gly Thr Asp Thr Tyr Leu Leu Gly Glu Pro 
                565                 570                 575     


Thr Leu Pro Gln His Ser Leu Leu Tyr Glu Glu Tyr Met Val Leu Ser 
            580                 585                 590         


Glu Leu Asn Asn Val Arg Leu Asn Val Arg Gln Gly Asn His Trp Ser 
        595                 600                 605             


Asp Lys Asn Arg Gln Arg Leu Ser Tyr Lys Gln Lys Glu Phe Leu Ile 
    610                 615                 620                 


Glu Asn Leu Phe Lys Glu His Lys Thr Val Thr Arg Lys Arg Ala Glu 
625                 630                 635                 640 


Asp Cys Leu Ser Ser Met Gly Cys Gly Glu Val Gln Leu Phe Gly Leu 
                645                 650                 655     


Ser Asp Glu Lys Lys Phe Ala Ser Ser Leu Ser Ser Tyr Ile Thr Leu 
            660                 665                 670         


Lys Lys Ile Leu Gly Lys Ala Phe Val Asp Asp Ser Gly Asn Ser Ala 
        675                 680                 685             


Ile Leu Glu Arg Ile Ile Glu Val Gln Thr Val Phe Glu Asp Arg Glu 
    690                 695                 700                 


Pro Leu Thr His Gln Leu Ser Leu Ile Asp Ser Leu Thr Val Glu Gln 
705                 710                 715                 720 


Arg Lys Gln Leu Ala Lys Ile His Tyr Thr Gly Trp Gly Lys Leu Ser 
                725                 730                 735     


Arg Lys Leu Leu Thr Ser Lys Lys Gly Ser Tyr Lys Ile His Ser Gln 
            740                 745                 750         


Asn Glu Ile Met Arg Thr Gln His Ser Ile Ile Asp Ile Met Arg Gly 
        755                 760                 765             


Asp Ser Leu Asn Leu Ile Glu Ile Ile Arg Asp Lys Lys Ile Gly Ile 
    770                 775                 780                 


Gly Glu Trp Ile Asp Asn Glu Asn Leu Asp Arg Glu Thr Gly Glu Ser 
785                 790                 795                 800 


Asp Val Ser Arg Leu Glu Ser Val Phe Glu Gly Ile Val Met Ser Pro 
                805                 810                 815     


Arg Val Lys Arg Gly Val Val Gln Ala Val Arg Val Ile Asp Asp Leu 
            820                 825                 830         


Ala Lys Ala Ile Gly Asn Pro Pro Gln Arg Ile Phe Ile Glu Met Ala 
        835                 840                 845             


Asp Glu Val Gln Ala Ser Gln Arg Thr Val Ser Arg Leu Ser Arg Leu 
    850                 855                 860                 


Lys Ala Leu Tyr Gly Ser Pro Ser Leu Gln Lys Glu Phe Asn Asp Leu 
865                 870                 875                 880 


Lys Asp Leu Arg Lys Ser Leu Asn Glu Thr Lys Asp Ala Asp Leu Gln 
                885                 890                 895     


Glu Asp Arg Leu Tyr Leu Tyr Tyr Ile Gln Leu Gly Lys Asp Leu Tyr 
            900                 905                 910         


Thr Gly Lys Glu Ile Ser Ile Asp Ser Leu Ser Ser Ala Tyr Asp Ile 
        915                 920                 925             


Asp His Ile Val Pro Gln Ala Leu Thr Gln Asp Asp Ser Ile Asp Asn 
    930                 935                 940                 


Arg Val Leu Val Ser Arg Lys Glu Asn Ala Arg Lys Thr Asp Ser Tyr 
945                 950                 955                 960 


Leu Tyr Thr Pro Asp Leu Ile Lys Asn Met Arg Pro Leu Trp Gly Lys 
                965                 970                 975     


Leu Leu Ala Ala Gly Leu Met Ser Gln Lys Lys Tyr Asp Ala Leu Thr 
            980                 985                 990         


Arg Val Asp Asp Phe Lys Asp Arg  Glu Lys Gln Arg Phe  Val Gln Arg 
        995                 1000                 1005             


Ser Leu  Val Glu Thr Arg Gln  Ile Met Lys Asn Val  Ser Thr Ile 
    1010                 1015                 1020             


Leu Arg  Thr Tyr Tyr Gly Glu  Asp Thr Asn Val Val  Thr Leu Lys 
    1025                 1030                 1035             


Ser Ser  Ile Thr His Asp Met  Arg Lys Tyr Leu Gly  Phe Pro Tyr 
    1040                 1045                 1050             


Lys Asn  Arg Asp Ile Asn Asp  Tyr His His Ala Gln  Asp Ala Leu 
    1055                 1060                 1065             


Cys Ile  Ser Ala Ala Gly Gln  Phe Met Leu Asn Arg  His Phe Val 
    1070                 1075                 1080             


Ser Gly  Gly Glu Val Thr Asp  Gly Ala Ser Asn Ala  Tyr Asn Met 
    1085                 1090                 1095             


Tyr Leu  Ala Glu Tyr Leu Gln  Glu Phe Arg Asp Ala  Lys Ala Arg 
    1100                 1105                 1110             


Gly Glu  Arg Lys Arg Ser Thr  Ala Phe Gly Phe Val  Val Gly Ser 
    1115                 1120                 1125             


Met Ser  Ser Lys Asp Asp Glu  Lys Arg Thr Asp His  Arg Thr Gly 
    1130                 1135                 1140             


Glu Ile  Val Trp Ser Glu Thr  Asn Ala Glu Tyr Leu  Arg Lys Val 
    1145                 1150                 1155             


Met Asn  Tyr Lys Lys Met Leu  Ile Thr Gln Lys Val  Gly Asp Val 
    1160                 1165                 1170             


Lys Thr  Arg Ala Leu Tyr Asp  Glu Thr Arg Tyr Gly  His Lys Glu 
    1175                 1180                 1185             


Ser Ser  Ala Lys Ser Ile Lys  Phe Asp Asn Leu Arg  Arg Asp Thr 
    1190                 1195                 1200             


Ser Leu  Tyr Gly Gly Phe Ser  Gly Ala Ser Thr Ala  Tyr Met Met 
    1205                 1210                 1215             


Leu Val  Glu Tyr Lys Lys Lys  Asn Lys Leu Val Ser  Ile Thr Ala 
    1220                 1225                 1230             


Gln Glu  Ala Ser Leu Leu Ala  Val Ala Asp Gln Lys  Asn Asp Thr 
    1235                 1240                 1245             


Val Ser  Val Met Asp Gly Ile  Leu Ala Ser His Gly  Leu Ser Gly 
    1250                 1255                 1260             


Ala Lys  Ile Leu Leu Pro Lys  Ile Val Thr Gly Gln  Leu Leu Asp 
    1265                 1270                 1275             


Tyr Ser  Asp Ala Leu Val Thr  Leu Gln Ser Ala Ser  Glu Leu Asn 
    1280                 1285                 1290             


Thr Ala  Arg Gln Met Trp Leu  Pro Arg Asn Asp Tyr  Asn Asp Ile 
    1295                 1300                 1305             


Asp Ile  Ile Leu Lys Ser Lys  Asp Ile Glu Asp Ala  Ala His Lys 
    1310                 1315                 1320             


Leu Gly  Tyr Asp Ser Asp Thr  Val His Val Leu Arg  Arg Ile Gln 
    1325                 1330                 1335             


Lys Leu  Phe Glu Ala Val Phe  Arg Val Ala Glu Ser  Gln Phe Pro 
    1340                 1345                 1350             


Leu His  Gln Leu Ser Lys Asp  Gln Gln Glu Glu Ile  Leu Arg Asp 
    1355                 1360                 1365             


Pro Gln  Asn Tyr Gly Phe Gly  Ser Ala Val Lys Leu  Leu Gly Gly 
    1370                 1375                 1380             


Leu Leu  Lys Ala Leu His Ala  Asn Ala Glu Asn Thr  Asn Leu Ala 
    1385                 1390                 1395             


Gly Leu  Val Lys Val Leu His  Gly Asp Lys Glu Tyr  Ser Asp Asp 
    1400                 1405                 1410             


Gly Lys  Ala Lys Glu Phe Lys  Lys Phe Gly Phe Ser  Pro Arg Trp 
    1415                 1420                 1425             


Arg Arg  Ile Ser Asn Pro Thr  Gly Phe Thr Leu Ser  Asp Ser Asp 
    1430                 1435                 1440             


Thr Phe  Ile Tyr Gln Ser Pro  Ser Gly Ile Phe Glu  Gln Arg Val 
    1445                 1450                 1455             


Thr Val  Lys Glu Leu Lys Glu  Lys Ala Asp Lys Gln  Lys 
    1460                 1465                 1470     


<210>  1281
<211>  1473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1281

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Lys Asp Thr Asp Gln Lys Asn Gly Asn Ile Leu Gly 
            20                  25                  30          


Thr Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Lys Asp Glu Phe 
        35                  40                  45              


Gly Lys Gly Asn Ser Ile Ser Gln Thr Ala Asp Arg Thr Lys Phe Arg 
    50                  55                  60                  


Ser Val Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Asn Val Leu Gly Phe Leu Pro Lys His Tyr Ala Asp 
                85                  90                  95      


Gln Ile Asp Phe Lys Lys Arg Leu Gly Lys Leu Val Asn Glu Thr Glu 
            100                 105                 110         


Pro Lys Leu Val Tyr Asp Asn Lys Asn Phe Ile Phe Lys Lys Ser Phe 
        115                 120                 125             


Glu Ala Met Leu Ser Asp Phe Asn Gln His Gln Pro Leu Leu Leu Ala 
    130                 135                 140                 


Asp Gly Lys Lys Val Pro Phe Asp Trp Thr Ile Tyr Tyr Leu Arg Lys 
145                 150                 155                 160 


Lys Ala Leu Thr Glu Lys Ile Glu Lys Glu Glu Leu Ala Trp Leu Ile 
                165                 170                 175     


Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu 
            180                 185                 190         


Glu Glu Glu Asn Pro Asn Lys Met Val Ala Phe His Ser Leu Lys Ile 
        195                 200                 205             


Val Glu Val Ile Ala Asp Glu Ser Gln Lys Gly Lys Ala Glu Thr Trp 
    210                 215                 220                 


Tyr Ser Leu Ile Leu Glu Asn Gly Trp Ile Tyr Arg Arg Ser Ser Lys 
225                 230                 235                 240 


Thr Pro Leu Tyr Asp Trp Lys Gly Lys Gln Arg Asp Phe Ile Val Thr 
                245                 250                 255     


Thr Asp Leu Asn Asp Asp Gly Ser Ile Lys Thr Asp Lys Glu Gly Leu 
            260                 265                 270         


Glu Lys Arg Ser Phe Arg Ala Pro Val Glu Asp Asp Trp Thr Leu Val 
        275                 280                 285             


Lys Lys Lys Thr Glu Gln Glu Ile Asn Arg Ser His Lys Thr Val Gly 
    290                 295                 300                 


Thr Tyr Ile Tyr Glu Asn Leu Leu Gln Asp Pro Lys Gln Lys Ile Lys 
305                 310                 315                 320 


Gly Lys Leu Val Arg Thr Ile Glu Arg Glu Phe Tyr Lys Asp Glu Leu 
                325                 330                 335     


Lys Met Ile Leu Lys Asn Gln Gln Lys Phe His Pro Glu Leu Asn Asp 
            340                 345                 350         


Glu His Leu Tyr Ile Asn Cys Val Arg Glu Leu Tyr Arg Asn Asn Gln 
        355                 360                 365             


Ala His Gln Thr Ile Leu Ser Lys Lys Asp Phe Val His Leu Phe Ile 
    370                 375                 380                 


Asp Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser Gln Lys Ser Ser 
385                 390                 395                 400 


Ile Ser Asn Cys Thr Leu Glu Phe Arg Lys Tyr Lys Asn Glu Asn Gly 
                405                 410                 415     


Thr Asp Gln Thr Asp Tyr Leu Lys Ala Ile Pro Lys Ser His Pro Leu 
            420                 425                 430         


Phe Gln Glu Phe Arg Leu Trp Gln Trp Ile Tyr Asn Leu Gly Ile Tyr 
        435                 440                 445             


Gln Lys Glu Asp Asp Thr Asn Val Thr Asp Glu Leu Ile Lys Asn Ile 
    450                 455                 460                 


Asp Gly Tyr Gln Asp Leu Phe Asp Phe Leu Asn Asn Lys Lys Glu Ile 
465                 470                 475                 480 


Arg Gln Asp Ile Leu Ile Lys Phe Leu Val Glu Gln Thr Gly Leu Lys 
                485                 490                 495     


Gly Lys Pro Leu Ser Ser Val Val Ala Lys Tyr Arg Trp Asn Phe Val 
            500                 505                 510         


Glu Asp Lys Ile Tyr Pro Cys Asn Glu Thr Gly Thr Gln Ile Arg Thr 
        515                 520                 525             


Arg Leu Ala Lys Val Glu Asn Thr Pro Glu Asp Phe Leu Thr Ala Glu 
    530                 535                 540                 


Thr Glu Ile Lys Leu Trp His Ile Ile Tyr Ser Val Asn Asp Lys Ala 
545                 550                 555                 560 


Asp Tyr Glu Lys Ala Leu Lys Thr Phe Ala Glu Lys His Asn Leu Asp 
                565                 570                 575     


Thr His Ser Phe Val Glu Asn Phe Lys Lys Phe Pro Pro Phe Lys Ser 
            580                 585                 590         


Glu Tyr Gly Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu 
        595                 600                 605             


Ile Arg Leu Gly Lys Tyr Trp Ser Trp Glu Asn Ile Asp Lys Lys Thr 
    610                 615                 620                 


Gln Glu Arg Ile Glu Lys Ile Leu Thr Gly Glu Phe Asp Glu Ala Ile 
625                 630                 635                 640 


Lys Ser Arg Val Arg Asp Lys Ala Ile His Leu Thr Glu Glu Asp His 
                645                 650                 655     


Phe Gln Gly Leu Gln Leu Trp Leu Ala Ser Tyr Val Val Tyr Gly Arg 
            660                 665                 670         


His Ser Glu Ala Asp Asn Leu Asp Lys Trp His Thr Val Ala Asp Leu 
        675                 680                 685             


Glu Asp Tyr Leu Ala Glu Phe Arg Gln His Ser Leu Arg Asn Pro Ile 
    690                 695                 700                 


Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val Val Lys Asp Ile Trp 
705                 710                 715                 720 


Leu Gln Tyr Gly Gln Gly Ala Lys Asp Phe Phe Ser Glu Ile His Ile 
                725                 730                 735     


Glu Leu Ala Arg Glu Met Lys Asn Thr Gly Asp Glu Arg Lys Arg Leu 
            740                 745                 750         


Thr Ser Leu Val Thr Asp Asn Glu Asn Thr Asn Leu Arg Ile Lys Val 
        755                 760                 765             


Leu Leu Ser Glu Leu Leu Asn Asp Gly Asn Val Glu Asn Val Arg Pro 
    770                 775                 780                 


Tyr Ser Pro Met Gln Gln Glu Ala Leu Lys Ile Phe Glu Asp Gly Val 
785                 790                 795                 800 


Leu Asn Ser Asp Ile Glu Ile Glu Asp Asp Ile Leu Lys Ile Ser Lys 
                805                 810                 815     


Met Ala Gln Pro Thr Asn Thr Glu Leu Lys Arg Tyr Lys Leu Trp Leu 
            820                 825                 830         


Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Gln Ile Ile Pro Leu Asn 
        835                 840                 845             


Arg Leu Phe Thr Ala Asp Tyr Glu Ile Glu His Ile Ile Pro Gln Ser 
    850                 855                 860                 


Arg Phe Phe Asp Asp Ser Phe Ser Asn Lys Ile Ile Cys Glu Ser Ala 
865                 870                 875                 880 


Val Asn Gln Leu Lys Asp Lys Gln Leu Gly Leu Glu Phe Ile Lys Asn 
                885                 890                 895     


His Ser Gly Thr Ile Val Lys Leu Gly Met Gly Lys Thr Ala Lys Ile 
            900                 905                 910         


Leu Asp Val Glu Ser Tyr Asn Glu Phe Val Lys Gly Asn Tyr Thr Lys 
        915                 920                 925             


Asn Ile Ser Lys Arg Asn Lys Leu Met Leu Glu Glu Ile Pro Asp Lys 
    930                 935                 940                 


Met Ile Glu Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser Lys Phe Ile 
945                 950                 955                 960 


Ile Ser Ala Leu Ser Asn Ile Val Arg Ser Glu Ser Asn Asp Asp Gly 
                965                 970                 975     


Phe Asn Ser Lys Asn Val Leu Pro Gly Asn Gly Lys Ile Thr Ser Arg 
            980                 985                 990         


Leu Lys Gln Asp Trp Gly Met Asn  Asp Ile Trp Asn Glu  Leu Ile Leu 
        995                 1000                 1005             


Pro Arg  Phe Glu Arg Met Asn  Lys Ile Thr Asp Ser  Ala Val Phe 
    1010                 1015                 1020             


Thr Ala  Trp Asn Glu Asn Asn  Gln Lys Phe Leu Pro  Ile Val Pro 
    1025                 1030                 1035             


Leu Glu  Phe Ser Lys Gly Phe  Gln Lys Lys Arg Ile  Asp His Arg 
    1040                 1045                 1050             


His His  Ala Leu Asp Ala Leu  Val Ile Ala Ala Ser  Thr Arg Glu 
    1055                 1060                 1065             


His Ile  Asn Leu Leu Asn Asn  Glu His Ala Lys Ser  Asp Lys Arg 
    1070                 1075                 1080             


Phe Asp  Leu Asn Arg Lys Leu  Arg Lys Phe Glu Lys  Val Ala Tyr 
    1085                 1090                 1095             


Asn Asp  Pro Lys Thr Gly Lys  Arg Ile Glu Lys Glu  Val Pro Thr 
    1100                 1105                 1110             


Ile Phe  Leu Lys Pro Trp Glu  Thr Phe Thr Val Asp  Ala Lys Asn 
    1115                 1120                 1125             


Ser Leu  Glu Ser Ile Ile Val  Ser Phe Lys Gln Asn  Leu Arg Val 
    1130                 1135                 1140             


Ile Asn  Arg Ala Thr Asn Tyr  Ser Glu Ser Tyr Asn  Asp Glu Asn 
    1145                 1150                 1155             


Gly Ala  Leu Arg Leu Gly Lys  Asp Gly Asn Pro Leu  Lys Glu Lys 
    1160                 1165                 1170             


Ile Lys  Gln Ser Lys Gly Asp  Ser Trp Ala Ile Arg  Lys Pro Met 
    1175                 1180                 1185             


His Lys  Asp Phe Val Tyr Gly  Lys Val His Leu Gln  Asn Val Lys 
    1190                 1195                 1200             


Leu Pro  Lys Asp Lys Phe Ile  Thr Ala Ile Arg Lys  Ser Leu Asp 
    1205                 1210                 1215             


Val Thr  Phe Asp Leu Lys Arg  Ile Glu Thr Ile Thr  Asp Thr Gly 
    1220                 1225                 1230             


Ile Gln  Lys Ile Leu Lys Asn  Tyr Leu His Ser Lys  Glu Asn Lys 
    1235                 1240                 1245             


Val Glu  Leu Ala Phe Ser Pro  Glu Gly Ile Glu Asp  Met Asn Gln 
    1250                 1255                 1260             


His Ile  Glu Arg Tyr Asn Asp  Gly Lys Lys His Gln  Pro Ile His 
    1265                 1270                 1275             


Lys Val  Arg Val Phe Glu Ile  Gly Ser Arg Phe Ser  Leu Gly Glu 
    1280                 1285                 1290             


Ser Gly  Asn Lys Lys Thr Lys  Phe Val Glu Ala Ala  Lys Gly Thr 
    1295                 1300                 1305             


Asn Leu  Phe Phe Ala Val Tyr  Gln Asp Asp Asn Lys  Gly Lys Arg 
    1310                 1315                 1320             


Asn Tyr  Glu Thr Ile Pro Leu  Asn Ile Val Ile Glu  Arg Gln Lys 
    1325                 1330                 1335             


Leu Gly  Leu His Ser Val Pro  Thr Glu Asp Ala Lys  Gly Gly Lys 
    1340                 1345                 1350             


Leu Leu  Phe His Leu Ser Pro  Asn Asp Met Val Tyr  Val Pro Thr 
    1355                 1360                 1365             


Ser Glu  Glu Leu Glu Asp Asn  Leu Ala Ser Val Asn  Ile Lys Arg 
    1370                 1375                 1380             


Leu Asn  Thr Ala Gln Arg Lys  Arg Ile Tyr Lys Val  Glu Lys Ala 
    1385                 1390                 1395             


Ser Gly  Val Glu Cys Tyr Phe  Ile Arg Gln Asp Ile  Ala Tyr Leu 
    1400                 1405                 1410             


Ile Lys  Asn Tyr Asp Ala Lys  Thr Lys Ile Gly Glu  Leu Glu Ser 
    1415                 1420                 1425             


Gln Asn  Lys Leu Gln Thr Thr  Met Thr Asp Asp Arg  Leu Lys Ile 
    1430                 1435                 1440             


Thr Asp  Val Cys Val Lys Leu  Asn Ile Asp Arg Leu  Gly Ser Ile 
    1445                 1450                 1455             


Ala Glu  Thr Phe Leu Gly Asn  Gln Tyr Gly Ala Ser  Lys Lys Thr 
    1460                 1465                 1470             


<210>  1282
<211>  1473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1282

Met Ala Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Glu Arg Glu Asn Ile Asp Phe Ser Leu Ile Asp Lys Gly 
            20                  25                  30          


Val Arg Ile Phe Ser Glu Gly Val Lys Ser Glu Lys Gly Ile Glu Ser 
        35                  40                  45              


Ser Arg Ala Ala Glu Arg Thr Gly Tyr Arg Ser Ala Arg Lys Ile Lys 
    50                  55                  60                  


Tyr Arg Arg Lys Leu Arg Lys Tyr Glu Thr Leu Lys Val Leu Ser Leu 
65                  70                  75                  80  


Asn Arg Met Cys Pro Leu Ser Ile Glu Glu Val Glu Glu Trp Lys Lys 
                85                  90                  95      


Ser Gly Phe Lys Asp Tyr Pro Leu Asn Pro Glu Phe Leu Lys Trp Leu 
            100                 105                 110         


Ser Thr Asp Glu Glu Ser Asn Val Asn Pro Tyr Phe Phe Arg Asp Arg 
        115                 120                 125             


Ala Ser Lys His Lys Val Ser Leu Phe Glu Leu Gly Arg Ala Phe Tyr 
    130                 135                 140                 


His Ile Ala Gln Arg Arg Gly Phe Leu Ser Asn Arg Leu Asp Gln Ser 
145                 150                 155                 160 


Ala Glu Gly Ile Leu Glu Glu His Cys Pro Lys Ile Glu Ala Ile Val 
                165                 170                 175     


Glu Asp Leu Ile Ser Ile Asp Glu Ile Ser Thr Asn Ile Thr Asp Tyr 
            180                 185                 190         


Phe Phe Glu Thr Gly Ile Leu Asp Ser Asn Glu Lys Asn Gly Tyr Ala 
        195                 200                 205             


Lys Asp Leu Asp Glu Gly Asp Lys Lys Leu Val Ser Leu Tyr Lys Ser 
    210                 215                 220                 


Leu Leu Ala Ile Leu Lys Lys Asn Glu Ser Asp Phe Glu Asn Cys Lys 
225                 230                 235                 240 


Ser Glu Ile Ile Glu Arg Leu Asn Lys Lys Asp Val Leu Gly Lys Val 
                245                 250                 255     


Lys Gly Lys Ile Lys Asp Ile Ser Gln Ala Met Leu Asp Gly Asn Tyr 
            260                 265                 270         


Lys Thr Leu Gly Gln Tyr Phe Tyr Ser Leu Tyr Ser Lys Glu Lys Ile 
        275                 280                 285             


Arg Asn Gln Tyr Thr Ser Arg Glu Glu His Tyr Leu Ser Glu Phe Ile 
    290                 295                 300                 


Thr Ile Cys Lys Val Gln Gly Ile Asp Gln Ile Asn Glu Glu Glu Lys 
305                 310                 315                 320 


Ile Asn Glu Lys Lys Phe Asp Gly Leu Ala Lys Asp Leu Tyr Lys Ala 
                325                 330                 335     


Ile Phe Phe Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Ile Gly Lys 
            340                 345                 350         


Cys Ser Phe Glu Lys Ser Lys Ser Arg Cys Ala Ile Ser His Pro Asp 
        355                 360                 365             


Phe Glu Glu Tyr Arg Met Trp Thr Tyr Leu Asn Thr Ile Lys Ile Gly 
    370                 375                 380                 


Thr Gln Ser Asp Lys Lys Leu Arg Phe Leu Thr Gln Asp Glu Lys Leu 
385                 390                 395                 400 


Lys Leu Val Pro Lys Phe Tyr Arg Lys Asn Asp Phe Asn Phe Asp Val 
                405                 410                 415     


Leu Ala Lys Glu Leu Ile Glu Lys Gly Ser Ser Phe Gly Phe Tyr Lys 
            420                 425                 430         


Ser Ser Lys Lys Asn Asp Phe Phe Tyr Trp Phe Asn Tyr Lys Pro Thr 
        435                 440                 445             


Asp Thr Val Ala Ala Cys Gln Val Ala Ala Ser Leu Lys Asn Ala Ile 
    450                 455                 460                 


Gly Glu Asp Trp Lys Thr Lys Ser Phe Lys Tyr Gln Thr Ile Asn Ser 
465                 470                 475                 480 


Asn Lys Glu Gln Val Ser Arg Thr Val Asp Tyr Lys Asp Leu Trp His 
                485                 490                 495     


Leu Leu Thr Val Ala Thr Ser Asp Val Tyr Leu Tyr Glu Phe Ala Ile 
            500                 505                 510         


Asp Lys Leu Gly Leu Asp Glu Lys Asn Ala Lys Ala Phe Ser Lys Thr 
        515                 520                 525             


Lys Leu Lys Lys Asp Phe Ala Ser Leu Ser Leu Ser Ala Ile Asn Lys 
    530                 535                 540                 


Ile Leu Pro Tyr Leu Lys Glu Gly Leu Leu Tyr Ser His Ala Val Phe 
545                 550                 555                 560 


Val Ala Asn Ile Glu Asn Ile Val Asp Glu Asn Ile Trp Lys Asp Glu 
                565                 570                 575     


Lys Gln Arg Asp Tyr Ile Lys Thr Gln Ile Ser Glu Ile Ile Glu Asn 
            580                 585                 590         


Tyr Thr Leu Glu Lys Ser Arg Phe Glu Ile Ile Asn Gly Leu Leu Lys 
        595                 600                 605             


Glu Tyr Lys Ser Glu Asn Glu Asp Gly Lys Arg Val Tyr Tyr Ser Lys 
    610                 615                 620                 


Glu Ala Glu Gln Ser Phe Glu Asn Asp Leu Lys Lys Lys Leu Val Leu 
625                 630                 635                 640 


Phe Tyr Lys Ser Asn Glu Ile Glu Asn Lys Glu Gln Gln Glu Thr Ile 
                645                 650                 655     


Phe Asn Glu Leu Leu Pro Ile Phe Ile Gln Gln Leu Lys Asp Tyr Glu 
            660                 665                 670         


Phe Ile Lys Ile Gln Arg Leu Asp Gln Lys Val Leu Ile Phe Leu Lys 
        675                 680                 685             


Gly Lys Asn Glu Thr Gly Gln Ile Phe Cys Thr Glu Glu Lys Gly Thr 
    690                 695                 700                 


Ala Glu Glu Lys Glu Lys Lys Ile Lys Asn Arg Leu Lys Lys Leu Tyr 
705                 710                 715                 720 


His Pro Ser Asp Ile Glu Lys Phe Lys Lys Lys Ile Ile Lys Asp Glu 
                725                 730                 735     


Phe Gly Asn Glu Lys Ile Val Leu Gly Ser Pro Leu Thr Pro Ser Ile 
            740                 745                 750         


Lys Asn Pro Met Ala Met Arg Ala Leu His Gln Leu Arg Lys Val Leu 
        755                 760                 765             


Asn Ala Leu Ile Leu Glu Gly Gln Ile Asp Glu Lys Thr Ile Ile His 
    770                 775                 780                 


Ile Glu Met Ala Arg Glu Leu Asn Asp Ala Asn Lys Arg Lys Gly Ile 
785                 790                 795                 800 


Gln Asp Tyr Gln Asn Asp Asn Lys Lys Phe Arg Glu Asp Ala Ile Lys 
                805                 810                 815     


Glu Ile Lys Lys Leu Tyr Phe Glu Asp Cys Lys Lys Glu Val Glu Pro 
            820                 825                 830         


Thr Glu Asp Asp Ile Leu Arg Tyr Gln Leu Trp Met Glu Gln Asn Arg 
        835                 840                 845             


Ser Glu Ile Tyr Glu Glu Gly Lys Asn Ile Ser Ile Cys Asp Ile Ile 
    850                 855                 860                 


Gly Ser Asn Pro Ala Tyr Asp Ile Glu His Thr Ile Pro Arg Ser Arg 
865                 870                 875                 880 


Ser Gln Asp Asn Ser Gln Met Asn Lys Thr Leu Cys Ser Gln Arg Phe 
                885                 890                 895     


Asn Arg Glu Val Lys Lys Gln Ser Met Pro Ile Glu Leu Asn Asn His 
            900                 905                 910         


Leu Glu Ile Leu Pro Arg Ile Ala His Trp Lys Glu Glu Ala Asp Asn 
        915                 920                 925             


Leu Thr Arg Glu Ile Glu Ile Ile Ser Arg Ser Ile Lys Ala Ala Ala 
    930                 935                 940                 


Thr Lys Glu Ile Lys Asp Lys Lys Ile Arg Arg Arg His Tyr Leu Thr 
945                 950                 955                 960 


Leu Lys Arg Asp Tyr Leu Gln Gly Lys Tyr Asp Arg Phe Ile Trp Glu 
                965                 970                 975     


Glu Pro Lys Val Gly Phe Lys Asn Ser Gln Ile Pro Asp Thr Gly Ile 
            980                 985                 990         


Ile Thr Lys Tyr Ala Gln Ala Tyr  Leu Lys Ser Tyr Phe  Lys Lys Val 
        995                 1000                 1005             


Glu Ser  Val Lys Gly Gly Met  Val Ala Glu Phe Arg  Lys Ile Trp 
    1010                 1015                 1020             


Gly Ile  Gln Glu Ser Phe Ile  Asp Glu Asn Gly Met  Lys His Tyr 
    1025                 1030                 1035             


Lys Val  Lys Asp Arg Ser Lys  His Thr His His Thr  Ile Asp Ala 
    1040                 1045                 1050             


Ile Thr  Ile Ala Cys Met Thr  Lys Glu Lys Tyr Asp  Val Leu Ala 
    1055                 1060                 1065             


His Ala  Trp Thr Leu Glu Asp  Gln Gln Asn Lys Lys  Glu Ala Arg 
    1070                 1075                 1080             


Ser Ile  Ile Glu Ala Ser Lys  Pro Trp Lys Thr Phe  Lys Glu Asp 
    1085                 1090                 1095             


Leu Leu  Lys Ile Glu Glu Glu  Ile Leu Val Ser His  Tyr Thr Pro 
    1100                 1105                 1110             


Asp Asn  Val Lys Lys Gln Ala  Lys Lys Ile Val Arg  Val Arg Gly 
    1115                 1120                 1125             


Lys Lys  Gln Phe Val Ala Glu  Val Glu Arg Asp Val  Asn Gly Lys 
    1130                 1135                 1140             


Ala Val  Pro Lys Lys Ala Ala  Ser Gly Lys Thr Ile  Tyr Lys Leu 
    1145                 1150                 1155             


Asp Gly  Glu Gly Lys Lys Leu  Pro Arg Leu Gln Gln  Gly Asp Thr 
    1160                 1165                 1170             


Ile Arg  Gly Ser Leu His Gln  Asp Ser Ile Tyr Gly  Ala Ile Lys 
    1175                 1180                 1185             


Asn Pro  Leu Asn Thr Asp Glu  Ile Lys Tyr Val Ile  Arg Lys Asp 
    1190                 1195                 1200             


Leu Glu  Ser Ile Lys Gly Ser  Asp Val Glu Ser Ile  Val Asp Glu 
    1205                 1210                 1215             


Val Val  Lys Glu Lys Ile Lys  Glu Ala Ile Ala Asn  Lys Val Leu 
    1220                 1225                 1230             


Leu Leu  Ser Ser Asn Ala Gln  Gln Lys Asn Lys Leu  Val Gly Thr 
    1235                 1240                 1245             


Val Trp  Met Asn Glu Glu Lys  Arg Ile Ala Ile Asn  Lys Val Arg 
    1250                 1255                 1260             


Ile Tyr  Ala Asn Ser Val Lys  Asn Pro Leu His Ile  Lys Glu His 
    1265                 1270                 1275             


Ser Leu  Leu Ser Lys Ser Lys  His Val His Lys Gln  Lys Val Tyr 
    1280                 1285                 1290             


Gly Gln  Asn Asp Glu Asn Tyr  Ala Met Ala Ile Tyr  Glu Leu Asp 
    1295                 1300                 1305             


Gly Lys  Arg Asp Phe Glu Leu  Ile Asn Ile Phe Asn  Leu Ala Lys 
    1310                 1315                 1320             


Leu Ile  Lys Gln Gly Gln Gly  Phe Tyr Pro Leu His  Lys Lys Lys 
    1325                 1330                 1335             


Glu Ile  Lys Gly Lys Ile Val  Phe Val Pro Ile Glu  Lys Arg Asn 
    1340                 1345                 1350             


Lys Arg  Asp Val Val Leu Lys  Arg Gly Gln Gln Val  Val Phe Tyr 
    1355                 1360                 1365             


Asp Lys  Glu Val Glu Asn Pro  Lys Asp Ile Ser Glu  Ile Val Asp 
    1370                 1375                 1380             


Phe Lys  Gly Arg Ile Tyr Ile  Ile Glu Gly Leu Ser  Ile Gln Arg 
    1385                 1390                 1395             


Ile Val  Arg Pro Ser Gly Lys  Val Asp Glu Tyr Gly  Val Ile Met 
    1400                 1405                 1410             


Leu Arg  Tyr Phe Lys Glu Ala  Arg Lys Ala Asp Asp  Ile Lys Gln 
    1415                 1420                 1425             


Asp Asn  Phe Lys Pro Asp Gly  Val Phe Lys Leu Gly  Glu Asn Lys 
    1430                 1435                 1440             


Pro Thr  Arg Lys Met Asn His  Asn Gln Phe Thr Ala  Phe Val Glu 
    1445                 1450                 1455             


Gly Ile  Asp Phe Lys Val Leu  Pro Ser Gly Lys Phe  Glu Lys Ile 
    1460                 1465                 1470             


<210>  1283
<211>  1474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1283

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Ser Asp Phe Glu Lys Lys Gln Gly Lys Ile Asn Gly 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Lys Phe 
        35                  40                  45              


Asp Ser Gly Val Ser Ile Ser Gln Thr Ala Glu Arg Thr Ser Tyr Arg 
    50                  55                  60                  


Gly Val Arg Arg Leu Asn Gln Arg Asn Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Asn Ile Leu Asp Phe Leu Pro Lys His Tyr Ser Asn 
                85                  90                  95      


Ala Ile Asp Phe Glu Lys Lys Leu Gly Gln Phe Lys Glu Asn Val Glu 
            100                 105                 110         


Glu Lys Leu Asn Tyr Ala Lys Asn Gln Ile Gly Arg His Glu Phe Ile 
        115                 120                 125             


Phe Met Asp Ser Phe Lys Glu Met Val Lys Glu Phe Lys Gln Thr Gln 
    130                 135                 140                 


Pro Gln Leu Phe Tyr Lys Arg Glu Asn Gly Ile Glu Thr Lys Ile Pro 
145                 150                 155                 160 


Tyr Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Lys 
                165                 170                 175     


Ile Lys Lys Glu Glu Leu Ala Trp Ile Ile Leu Asn Phe Asn Gln Lys 
            180                 185                 190         


Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Asp Val Leu Asp Glu Ser Lys 
        195                 200                 205             


Ile Glu Asp Phe Tyr Ser Leu Lys Val Val Glu Val Gln Glu Thr Glu 
    210                 215                 220                 


Asp Lys Asn Ala Arg Gly Thr Trp Tyr Asn Val Ile Leu Glu Asn Asp 
225                 230                 235                 240 


Trp Val Tyr Lys Arg Gln Ser Lys Glu Ser Leu Asp Asn Trp Ile Gly 
                245                 250                 255     


Lys Asn Lys Glu Phe Ile Val Thr Thr Asn Lys Glu Lys Asp Gly Ser 
            260                 265                 270         


Leu Lys Leu Asp Lys Ala Gly Asn Val Lys Arg Asn Phe Arg Ala Val 
        275                 280                 285             


Asp Ser Glu Lys Asp Trp Ile Ala Ile Lys Lys Lys Thr Glu Gln Asp 
    290                 295                 300                 


Val Glu Ile Ser Lys His Thr Val Gly Gln Tyr Ile Tyr Glu Thr Leu 
305                 310                 315                 320 


Leu Gln Asn Ala Lys Gln Lys Ile Lys Gly Lys Leu Val Lys Thr Ile 
                325                 330                 335     


Glu Arg Lys Phe Tyr Lys Glu Glu Phe Ile Lys Ile Ile Lys Glu Gln 
            340                 345                 350         


Leu Lys His His Pro Glu Leu Gln Ser Lys Thr Leu Tyr Glu Thr Cys 
        355                 360                 365             


Ile Gln Glu Leu Tyr Pro Arg Asn Glu Ala His Gln Asn Asn Ile Lys 
    370                 375                 380                 


Ser Lys Gly Phe Glu Tyr Leu Phe Thr Asp Asp Ile Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Thr Ile Ser Gly Cys Gln Tyr Glu 
                405                 410                 415     


Thr Arg Leu Phe Lys Lys Thr Val Leu Asn Lys Val Thr Asn Lys Glu 
            420                 425                 430         


Asn Glu Ile Trp Gln Lys Glu Pro Leu Lys Ala Ile Ser Lys Ser His 
        435                 440                 445             


Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Leu Lys Asn Leu Lys 
    450                 455                 460                 


Ile Tyr Gln Lys Glu Asp Val Lys Gly Asn Leu Ile Asp His Asp Ile 
465                 470                 475                 480 


Thr Ser Asp Leu Leu Lys Thr Glu Ala Asp Trp Val Glu Leu Phe Asp 
                485                 490                 495     


Phe Leu Asn Ile Lys Lys Glu Ile Glu Gln Lys Gln Ile Ile Gln Phe 
            500                 505                 510         


Phe Ile Asn Asn Lys Gln Ile Glu Lys Gly Lys Lys Asn Asn Tyr Arg 
        515                 520                 525             


Trp Asn Tyr Val Glu Asp Lys Lys Tyr Pro Cys Asn Glu Thr Lys Ser 
    530                 535                 540                 


Gln Leu Ile Ser Arg Leu Lys Lys Val Lys Asn Leu Asp Leu Asn Leu 
545                 550                 555                 560 


Phe Phe Thr Ser Glu Thr Glu Leu Lys Leu Trp His Ile Ile Tyr Ser 
                565                 570                 575     


Val Lys Asp Lys Ile Gln Tyr Glu Lys Ala Ile Lys Asn Phe Ala Ile 
            580                 585                 590         


Lys Asn Asn Ile Asp Ile Asp Ser Phe Val Glu Asn Phe Lys Arg Phe 
        595                 600                 605             


Pro Pro Phe Lys Asn Glu Tyr Ala Ala Tyr Ser Glu Lys Ala Ile Lys 
    610                 615                 620                 


Arg Leu Leu Pro Leu Met Arg Met Gly Glu Tyr Trp Asn Glu Ser Glu 
625                 630                 635                 640 


Ile Lys Gln Glu Val Lys Asp Arg Ala Tyr Ser Ile Ile Glu Arg Leu 
                645                 650                 655     


Lys Gly Ile Asp Phe Asn Lys Asp Lys Ile Asp Glu Lys Ile Thr Asp 
            660                 665                 670         


Asp Asp Ile Lys Lys Gln Leu Leu Lys Ser Phe Ile Asp Phe Lys Asp 
        675                 680                 685             


Lys Asn Pro Leu Lys Gly Leu Asn Thr Tyr Gln Ala Cys Tyr Leu Val 
    690                 695                 700                 


Tyr Asn Arg His Ser Glu Val Asn Ser Ile Ala Tyr Trp Lys Ser Pro 
705                 710                 715                 720 


Asp Asp Ile Asn Lys Tyr Leu Glu Gly Phe Lys Gln His Ser Leu Arg 
                725                 730                 735     


Asn Pro Ile Val Glu Gln Val Val Thr Glu Thr Leu Arg Thr Val Arg 
            740                 745                 750         


Asp Ile Trp Gln Tyr Tyr Gly Lys Gly Lys Ala Ser Phe Phe Asn Glu 
        755                 760                 765             


Ile His Val Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Tyr Lys Arg 
    770                 775                 780                 


Lys Ala Ser Thr Asn Arg Val Thr Glu Asn Glu Asn Thr Asn His Arg 
785                 790                 795                 800 


Ile Lys Glu Ile Leu Lys Glu Leu Gln Asn Glu Gly Val Gln Glu Val 
                805                 810                 815     


Lys Ser Tyr Ser Arg Asn Gln Gln Glu Ile Leu Lys Ile Tyr Glu Glu 
            820                 825                 830         


Gly Ile Tyr Gln Ser Ile Asp Val Val Asp Asp Ala Ile Glu Lys Ile 
        835                 840                 845             


Arg Lys Asn Ser Ser Pro Thr Lys Ala Glu Ile Ile Lys Tyr Lys Leu 
    850                 855                 860                 


Trp Leu Glu Gln Gly Tyr Thr Ser Pro Tyr Thr Gly Lys Thr Ile Pro 
865                 870                 875                 880 


Met Ser Lys Leu Phe Thr Thr Asp Tyr Gln Ile Glu His Ile Ile Pro 
                885                 890                 895     


Gln Ser Leu Tyr Phe Asp Asn Ser Leu Ser Asn Lys Ile Ile Cys Glu 
            900                 905                 910         


Ser Glu Val Asn Gln Leu Lys Asp Asn Gln Thr Ala Tyr Glu Phe Ile 
        915                 920                 925             


Lys Lys His Gly Thr Glu Lys Val Ser Leu Gly Lys Asn Lys Thr Val 
    930                 935                 940                 


Glu Ile Phe Thr Leu Glu Gln Tyr Glu Lys His Cys Asn Gln Tyr Phe 
945                 950                 955                 960 


Lys Lys Asn Lys Asn Lys Leu Lys Lys Leu Leu Ser Glu Glu Ile Pro 
                965                 970                 975     


Glu Gly Phe Ile Asn Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys 
            980                 985                 990         


Phe Ile Lys Gly Leu Leu Gly Asn  Leu Val Arg Glu Glu  Asp Glu Gln 
        995                 1000                 1005             


Glu Val  Thr Thr Lys His Ile  Val Pro Val Thr Gly  Ala Ile Thr 
    1010                 1015                 1020             


Ser Lys  Leu Lys Gln Asp Trp  Gly Leu Asn Asp Lys  Trp Asn Glu 
    1025                 1030                 1035             


Leu Val  Ala Ser Arg Phe Gln  Arg Leu Asn Thr Met  Thr Asn Ser 
    1040                 1045                 1050             


Asn Asn  Phe Gly Tyr Trp Asp  Thr Lys Ile Asn Ala  Phe Arg Val 
    1055                 1060                 1065             


Gln Val  Pro Asp Glu Ile Ser  Lys Gly Phe Ser Lys  Lys Arg Ile 
    1070                 1075                 1080             


Asp His  Arg His His Ala Leu  Asp Ala Leu Val Ile  Ala Cys Cys 
    1085                 1090                 1095             


Thr Arg  Asn His Ile Asn Tyr  Leu Asn Ala Leu Asn  Ala Glu Lys 
    1100                 1105                 1110             


Lys Asn  Tyr Gly Leu Arg Asp  Leu Leu Leu Ile Lys  Asn Asp Lys 
    1115                 1120                 1125             


Asn His  Tyr Thr Lys Phe Phe  Lys His Pro Trp Asp  Gly Phe Pro 
    1130                 1135                 1140             


Val Glu  Ala Lys Ser Val Leu  Glu Lys Thr Ile Ile  Ser Phe Lys 
    1145                 1150                 1155             


Gln Asn  Thr Arg Val Ile Asn  Lys Thr Asn Asn Lys  Thr Trp Gln 
    1160                 1165                 1170             


Trp Ala  Glu Lys Glu Gly Gln  Leu Lys Lys Val Leu  Val Lys Gln 
    1175                 1180                 1185             


Thr Lys  Gly Asp Asn Trp Ala  Ile Arg Lys Pro Met  His Lys Glu 
    1190                 1195                 1200             


Thr Val  Ser Gly Lys Leu Asn  Ile Pro Thr Pro Lys  Gly Lys Ile 
    1205                 1210                 1215             


Ala Thr  Ala Ser Arg Val Ser  Leu Ser Glu Ile Lys  Thr Arg Lys 
    1220                 1225                 1230             


Gln Leu  Asp Lys Ile Thr Asp  Ile Ser Ile Gln Lys  Ile Leu Glu 
    1235                 1240                 1245             


Arg His  Leu Asn Asn Tyr Leu  Asp Asp Lys Gly Lys  Glu Lys Phe 
    1250                 1255                 1260             


Asp Leu  Ala Phe Asn Ala Asp  Gly Val Glu Glu Leu  Asn Lys Asn 
    1265                 1270                 1275             


Ile Lys  Val Leu Asn Lys Gly  Lys Asn His Gln Pro  Ile Tyr Lys 
    1280                 1285                 1290             


Val Arg  Leu Tyr Glu Ile Gly  Ser Arg Phe Lys Val  Gly Asn Ser 
    1295                 1300                 1305             


Gly Asn  Lys Lys Asp Lys Tyr  Val Glu Ala Ala Lys  Gly Thr Asn 
    1310                 1315                 1320             


Leu Phe  Phe Ala Ile Tyr Leu  Asn Glu Lys Asp Lys  Lys Arg Val 
    1325                 1330                 1335             


Tyr Glu  Thr Ile Pro Leu Asn  Glu Val Ile Glu His  Gln Lys Gln 
    1340                 1345                 1350             


Val Ser  Asn Leu Pro Lys Asn  Glu Arg Thr Pro Val  Pro Val Asn 
    1355                 1360                 1365             


His Thr  Lys Gly Lys Phe Leu  Phe Ser Leu Ser Pro  Asn Asp Leu 
    1370                 1375                 1380             


Val Tyr  Val Pro Thr Asp Glu  Glu Ile Asp Asn Pro  Ser Leu Val 
    1385                 1390                 1395             


Asn Phe  Asn Asn Leu Asn Lys  Lys Gln Val Glu Lys  Ile Tyr Lys 
    1400                 1405                 1410             


Thr Val  Ser Cys Ser Thr Tyr  Gln Cys Phe Phe Val  His Asn Thr 
    1415                 1420                 1425             


Ile Ser  Lys Pro Ile Gln Asn  Lys Val Glu Phe Ser  Ala Leu Asn 
    1430                 1435                 1440             


Lys Met  Glu Lys Ser Ile Asp  Gly Ile Met Ile Lys  Asp Arg Cys 
    1445                 1450                 1455             


Trp Lys  Leu Lys Met Asp Arg  Leu Gly Asn Ile Ile  Ser Val Ile 
    1460                 1465                 1470             


Arg 
    


<210>  1284
<211>  1480
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1284

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Val Cys Val Asp Asn Asp Gly Asn Tyr Leu Arg Asp Ile Lys 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Thr Leu Gly Asn Phe 
        35                  40                  45              


Asp Lys Gly Val Thr Asp Ser Pro Thr Ala Ala Arg Thr Gly Phe Arg 
    50                  55                  60                  


Gly Val Arg Arg Ile Arg Glu Arg Ala Leu Gln Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Val Met Lys Phe Leu Pro Pro His Tyr Glu Asn 
                85                  90                  95      


Ala Ile Gly Trp Asn Arg Glu Glu Ala Thr Thr Tyr Gly Lys Phe Leu 
            100                 105                 110         


Asn His Gly Glu Pro Lys Leu Ala Trp Glu Lys Gln Glu Asp Gly Ser 
        115                 120                 125             


Met Arg Phe Leu Phe Met Asp Ser Phe His Glu Met Met Ala Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gln Pro Gly Leu Val Ala Gly Gly Lys Lys Ile Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Ala Ile 
                165                 170                 175     


Arg Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Asp Glu Glu Asn Pro Ala Lys 
        195                 200                 205             


Arg Glu Glu Tyr His Glu Leu Lys Val Val Ser Val Glu Ala Ala Glu 
    210                 215                 220                 


Glu Gly Lys Gly Gly Asn Thr Trp Tyr Asn Val Thr Leu Glu Asn Gly 
225                 230                 235                 240 


Trp Ile Tyr Arg Arg Gln Ser Lys Val Glu Leu His Asp Trp Val Gly 
                245                 250                 255     


Lys Thr Arg Gln Phe Ile Val Thr Thr Glu Tyr Glu Lys Asp Gly Val 
            260                 265                 270         


Thr Ile Lys Lys Asp Lys Asn Gly Glu Val Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Pro Ser Glu Asp Asp Trp Gly Leu Arg Lys Lys Arg Thr Glu Ser Leu 
    290                 295                 300                 


Leu Glu Asp Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Asp His Ile 
305                 310                 315                 320 


Leu Ser Glu Pro Thr Asp Lys Ile Arg Gly Asn Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Lys Glu Leu Glu Ala Ile Leu Leu Glu Gln 
            340                 345                 350         


Thr Lys His His Lys Glu Leu Lys Asp Gln Asn Thr Leu Ile Ala Cys 
        355                 360                 365             


Ile Lys Glu Leu Tyr Gln Ser Asn Lys Pro His Gln Asp Ser Leu Met 
    370                 375                 380                 


Lys Lys Asp Met Thr Tyr Leu Leu Ile Thr Asp Leu Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asp Cys Pro Tyr Glu 
                405                 410                 415     


Tyr Tyr Glu Tyr Thr Ile Lys Glu Thr Gly Glu Ile Lys Arg Gln Gly 
            420                 425                 430         


Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Ile Ile Asn Leu Arg Leu Phe Asp Arg Thr Asp Asp Lys 
    450                 455                 460                 


Glu Val Thr Ser Glu Tyr Leu Ser Ser Met Glu Asp Tyr Thr Lys Leu 
465                 470                 475                 480 


Phe Thr Tyr Leu Asn Asp Arg Lys Asp Ile Asn Gln Glu Thr Leu Leu 
                485                 490                 495     


Lys Asp Phe Phe Lys Gln Lys Lys Val Lys Met Gly Met Glu Lys Glu 
            500                 505                 510         


Phe Pro Ile Arg Trp Asn Tyr Ile Glu Asp Lys Glu Lys Lys Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg His Glu Leu Leu Ala Ala Leu Asp Lys Ala Gly 
    530                 535                 540                 


Ile Asp His Ser Trp Leu Asp Asp Lys Glu Arg Phe Tyr Arg Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Val Glu Ser Lys Glu Glu Ala Glu Gly Ala Leu 
                565                 570                 575     


Arg Lys Leu Lys Asp Asn Asp Asp Phe Val Asn Ala Phe Leu Lys Val 
            580                 585                 590         


Lys Pro Phe Lys Lys Asp Tyr Gly Ala Tyr Ser Glu Lys Ala Val Lys 
        595                 600                 605             


Lys Leu Leu Ala Ile Met Arg Met Gly Gly Leu Trp Asn Glu Thr Asp 
    610                 615                 620                 


Ile Cys Glu Gln Thr Arg Asn Arg Ile Gln Ser Ile Ile Lys Gly Glu 
625                 630                 635                 640 


Ile Asp Glu Lys Ile Lys Glu Arg Met Gly Arg Thr Phe Cys Gln Leu 
                645                 650                 655     


Ser Asp Phe Gln Gly Leu Pro Glu Trp Leu Ala Cys Tyr Val Val Tyr 
            660                 665                 670         


Gly Arg His Ser Glu Ala Thr Asp Ile Gln Arg Trp Thr Lys Pro Glu 
        675                 680                 685             


Asn Leu Met Ala Tyr Ile Asn Gly Phe Lys Gln His Ser Leu Arg Asn 
    690                 695                 700                 


Pro Ile Val Glu Gln Cys Ile Leu Glu Thr Leu Arg Thr Val His Asp 
705                 710                 715                 720 


Ile Trp Lys Glu Ala Gly His Ile Asp Glu Ile His Val Glu Leu Gly 
                725                 730                 735     


Arg Ser Met Lys Ser Thr Ala Asp Gln Arg Ala Arg Met Ser Lys Ser 
            740                 745                 750         


Ile Leu Gln Asn Glu Asn Thr Asn Leu Arg Ile Lys Ser Leu Leu Met 
        755                 760                 765             


Glu Leu Lys Asn Ala Lys Glu Ile Glu Asn Val Arg Pro Tyr Ser Pro 
    770                 775                 780                 


Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala Leu Gln Glu 
785                 790                 795                 800 


Leu Thr Lys Glu Asp Ala Gln Tyr Pro Glu Ile Ser Lys Ile Ser Gln 
                805                 810                 815     


Met Ala Gln Pro Ser Ala Lys Glu Leu Leu Lys Tyr Arg Leu Trp Leu 
            820                 825                 830         


Glu Gln Lys Tyr Cys Ser Pro Tyr Thr Gly Lys Pro Ile Ser Leu Ser 
        835                 840                 845             


Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Ile Ile Pro Gln Ser 
    850                 855                 860                 


Arg Tyr Phe Asp Asp Ser Phe Thr Asn Lys Val Ile Cys Glu Thr Glu 
865                 870                 875                 880 


Val Asn Gln Leu Lys Ser Asn Met Leu Gly Tyr Glu Phe Ile Lys Lys 
                885                 890                 895     


His Ser Gly Glu Ile Val His Cys Thr Thr Ile Gly Asp Val Lys Ile 
            900                 905                 910         


Leu Asp Ala Lys Glu Tyr Tyr Asn Phe Val Thr Glu His Tyr Ala Asn 
        915                 920                 925             


Asn Arg Arg Lys Lys Glu Lys Leu Leu Leu Glu Asp Ile Pro Gln Glu 
    930                 935                 940                 


Phe Leu Asn Arg Gln Met Asn Asp Ser Arg Tyr Ile Ser Lys Lys Val 
945                 950                 955                 960 


Met Ala Ile Leu Ser Asn Ile Val Arg Glu Glu Asp Glu Gln Glu Thr 
                965                 970                 975     


Asn Ser Lys Phe Val Val Ser Cys Ser Gly Gly Ile Thr Asp Arg Leu 
            980                 985                 990         


Lys Gln Asp Trp Gly Leu Asn Asp  Val Trp Asn Ser Ile  Val Tyr Pro 
        995                 1000                 1005             


Arg Phe  Glu Arg Leu Asn Lys  Leu Thr Gln Thr Glu  Ala Phe Gly 
    1010                 1015                 1020             


Arg Trp  Glu Asn Lys Glu Gly  Lys Gln Val Phe Gln  Thr Ser Leu 
    1025                 1030                 1035             


Pro Leu  Glu Leu Gln Arg Gly  Phe Ser Lys Lys Arg  Ile Asp His 
    1040                 1045                 1050             


Arg His  His Ala Met Asp Ala  Leu Val Ile Ala Cys  Ala Ser Arg 
    1055                 1060                 1065             


Asn Ile  Ile Asn Tyr Leu Asn  Asn Glu Ser Ala Lys  Ser Gln Glu 
    1070                 1075                 1080             


Gln Arg  Glu Asp Leu Arg Lys  Lys Leu Cys Asp Lys  Asn Arg Ile 
    1085                 1090                 1095             


Ile Arg  Lys Pro Trp Glu Thr  Phe Thr Gln Asp Ala  Arg Thr Ala 
    1100                 1105                 1110             


Leu Glu  Asp Val Val Val Ser  Phe Lys Asn Tyr Val  Arg Val Ile 
    1115                 1120                 1125             


Asn Lys  Ala Thr Asn Tyr Tyr  Glu Lys Tyr Asp Glu  Ser Gly Lys 
    1130                 1135                 1140             


Lys Gly  Met Ala Glu Gln Lys  Gly Gln Asp Met Trp  Ala Ile Arg 
    1145                 1150                 1155             


Lys Pro  Met His Lys Glu Thr  Val Phe Gly Gln Val  Asn Leu Arg 
    1160                 1165                 1170             


Arg Lys  Val Thr Val Lys Leu  Lys Asp Ala Leu Glu  Asn Ile Pro 
    1175                 1180                 1185             


Ala Ile  Cys Asp Lys Ala Leu  Arg Asp Tyr Val Asn  Asp Leu Ile 
    1190                 1195                 1200             


Ala Lys  Lys Phe Asn Thr Lys  Gln Met Leu Ala His  Phe Lys Ser 
    1205                 1210                 1215             


Ile Asn  Tyr Arg Leu Asn Met  Lys Ser Val Asn Lys  Val Gly Ile 
    1220                 1225                 1230             


Trp Val  Phe Ser Asp Glu Lys  Glu Pro Met Val Ala  Thr Arg Lys 
    1235                 1240                 1245             


Ala Ile  Asp Ile Ser Phe Asp  Ala Lys Arg Ile Asp  Thr Ile Thr 
    1250                 1255                 1260             


Asp Thr  Gly Ile Gln Gln Ile  Leu Cys Lys Tyr Leu  Lys Ser Lys 
    1265                 1270                 1275             


Gly Asn  Asp Pro Asn Val Ala  Phe Thr Pro Glu Gly  Ile Ala Glu 
    1280                 1285                 1290             


Met Asn  Lys His Ile Ile Asp  Tyr Asn Asn Gly Lys  Gln His Gln 
    1295                 1300                 1305             


Pro Ile  Leu Lys Val Arg Val  Ser Glu Pro Lys Gly  Ala Lys Tyr 
    1310                 1315                 1320             


Gln Val  Gly Glu Thr Gly Asn  Lys Thr Lys Lys Phe  Val Glu Ala 
    1325                 1330                 1335             


Gln Lys  Gly Thr Asn Leu Tyr  Phe Ala Ile Tyr Glu  Asp Lys Asp 
    1340                 1345                 1350             


Gly Asn  Arg Ser Tyr Asn Thr  Ile Pro Leu Asn Glu  Val Ala Glu 
    1355                 1360                 1365             


Arg Leu  Lys Gln Gly Leu Ser  Pro Val Pro Glu Lys  Asn Glu Lys 
    1370                 1375                 1380             


Gly Ile  Ser Leu Lys Phe Tyr  Leu Ser Pro Asn Asp  Leu Val Tyr 
    1385                 1390                 1395             


Val Pro  Thr Glu Glu Glu Lys  Leu Ser Asn Glu Cys  Lys Leu Ser 
    1400                 1405                 1410             


Lys Glu  Arg Ile Tyr Lys Met  Val Ser Ser Thr Ser  Asn Glu Cys 
    1415                 1420                 1425             


His Phe  Ile Pro Tyr Thr Thr  Ala Lys Pro Ile Tyr  Asp Lys Phe 
    1430                 1435                 1440             


Glu Tyr  Glu Ala Lys Asn Lys  Met Gly Arg Ala Leu  Thr Gly Glu 
    1445                 1450                 1455             


Met Ile  Lys Ala Val Cys Trp  Lys Leu Glu Val Asp  Arg Leu Gly 
    1460                 1465                 1470             


Asn Ile  Leu Lys Ile Ile Lys  
    1475                 1480 


<210>  1285
<211>  1483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1285

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Ile Ser Ile Asp Asp Asn Gly Asn Tyr Leu Arg Asp Ile Gln 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly Asn Phe 
        35                  40                  45              


Asp Ser Gly Val Thr Glu Ser Gln Thr Ala Val Arg Thr Gly Phe Arg 
    50                  55                  60                  


Gly Leu Arg Arg Ile Arg Glu Arg Ser Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Val Met Gly Phe Leu Pro Pro His Tyr Asp Ser 
                85                  90                  95      


Ala Ile Gly Trp Asn Lys Glu Glu Pro Thr Thr Tyr Gly Lys Phe Leu 
            100                 105                 110         


Asn His Gly Glu Pro Lys Leu Ala Trp Glu Arg Gln Gly Asp Gly Ser 
        115                 120                 125             


Met Arg Phe Leu Phe Met Asp Ser Phe His Lys Met Met Glu Asp Phe 
    130                 135                 140                 


Ala Pro His Gln Pro Ala Leu Val Ala Asp Gly Arg Lys Ile Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Pro Ile 
                165                 170                 175     


Lys Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu Asn Pro Thr Lys 
        195                 200                 205             


Arg Glu Glu Tyr His Glu Leu Lys Val Val Ser Val Glu Ala Ala Glu 
    210                 215                 220                 


Glu Gly Lys Gly Gly Asn Thr Trp Tyr Asn Val Lys Leu Glu Asn Gly 
225                 230                 235                 240 


Trp Ile Tyr Arg Arg Ser Ser Lys Ile Pro Leu Asp Asp Trp Val Gly 
                245                 250                 255     


Lys Thr Arg Pro Phe Ile Val Thr Thr Glu Tyr Glu Ala Asp Gly Ile 
            260                 265                 270         


Thr Leu Lys Lys Asn Lys Glu Gly Glu Val Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Pro Gly Glu Asp Asp Trp Gly Leu Arg Lys Lys Arg Thr Glu Ser Gln 
    290                 295                 300                 


Leu Glu Ala Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Asp His Leu 
305                 310                 315                 320 


Leu Ala Glu Pro Ala Asp Lys Ile Arg Gly Asn Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Gln Glu Leu Ala Asp Ile Leu Arg Glu Gln 
            340                 345                 350         


Ser Lys Tyr His Glu Glu Leu Gln Ser Lys Lys Ile Leu Ala Asp Cys 
        355                 360                 365             


Ala Glu Glu Leu Tyr Arg Ser Asn Gln Pro His Arg Asp Ser Leu Leu 
    370                 375                 380                 


Lys Lys Asp Met Val Tyr Leu Leu Leu Thr Asp Ile Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asp Cys Pro Tyr Glu 
                405                 410                 415     


Tyr Tyr Glu Tyr Val Asp Lys Glu Thr Gly Glu Ile Lys Arg Gln Gly 
            420                 425                 430         


Ile Lys Cys Val Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Val Leu Asn Leu Arg Leu Tyr Asp Arg Thr Glu Asp Lys 
    450                 455                 460                 


Glu Val Thr Ala Glu Tyr Leu Pro Ser Lys Glu Asp Tyr Ala Arg Leu 
465                 470                 475                 480 


Phe Val Tyr Leu Asn Asp Arg Lys Glu Ile Asn Gln Glu Thr Leu Leu 
                485                 490                 495     


Lys Asp Phe Phe Lys Leu Lys Lys Val Lys Gln Arg Ser Glu Lys Glu 
            500                 505                 510         


Phe Pro Val Arg Trp Asn Tyr Ile Glu Asp Lys Glu Lys Lys Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg His Glu Leu Leu Met Ala Leu Asp Arg Ala Gly 
    530                 535                 540                 


Ile Glu Arg Ser Trp Leu Asp Asp Thr Ala Arg Gln Cys Arg Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Ile Glu Val Lys Asp Glu Ala Glu Arg Ala Leu 
                565                 570                 575     


Arg Lys Leu Lys Asp Asp Asp Glu Phe Val Ser Ala Phe Leu Lys Val 
            580                 585                 590         


Lys Pro Phe Lys Lys Asp Tyr Gly Ala Tyr Ser Glu Lys Ala Val Lys 
        595                 600                 605             


Lys Leu Leu Ala Val Met Arg Met Gly Ser His Trp Cys Glu Asp Asp 
    610                 615                 620                 


Ile Cys Lys Glu Thr Lys Glu Arg Ile Glu His Ile Leu Gln Gly Asp 
625                 630                 635                 640 


Val Glu Glu Lys Ile Lys Glu Arg Met Asn His Thr Ala Tyr Ser Leu 
                645                 650                 655     


Gly Glu Leu Ser Asp Phe Gln Ala Leu Pro Glu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Val Val Tyr Gly Arg His Ser Glu Ala Thr Asp Ile Lys His Trp Lys 
        675                 680                 685             


Thr Pro Glu Ser Leu Met Ala Tyr Ile Asn Gly Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Cys Ile Leu Glu Thr Leu Arg Thr 
705                 710                 715                 720 


Val His Asp Ile Trp Lys Glu Ala Gly His Ile Asp Glu Ile His Val 
                725                 730                 735     


Glu Leu Gly Arg Ser Met Lys Ser Thr Ser Asp Gln Arg Ala Arg Met 
            740                 745                 750         


Thr Glu Asn Ile Leu Arg Asn Glu Asn Thr Asn Leu Arg Ile Lys Ser 
        755                 760                 765             


Leu Leu Met Glu Leu Lys Asn Asp Ala Asp Ile Lys Asp Val Arg Pro 
    770                 775                 780                 


Tyr Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala 
785                 790                 795                 800 


Leu Gln Glu Leu Thr Lys Glu Asp Asn Asp Tyr Glu Glu Ile Phe Lys 
                805                 810                 815     


Ile Ser Lys Met Pro Gln Pro Ser Ala Lys Glu Leu Leu Lys Tyr Lys 
            820                 825                 830         


Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Pro Ile 
        835                 840                 845             


Ser Leu Ser Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Ile Ile 
    850                 855                 860                 


Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Asn Asn Lys Val Ile Cys 
865                 870                 875                 880 


Glu Ser Glu Val Asn Ala Leu Lys Gly Asn Met Leu Gly Tyr Glu Phe 
                885                 890                 895     


Ile Lys Asn His Gly Gly Glu Ile Val His Cys Thr Leu Leu Gly Asp 
            900                 905                 910         


Val Lys Val Gln Asn Glu Ser Glu Tyr Lys Ala Phe Val Thr Glu His 
        915                 920                 925             


Tyr Ala Asn Asn Lys Tyr Lys Lys Glu Lys Leu Leu Met Glu Asp Ile 
    930                 935                 940                 


Pro Gln Asp Phe Leu Asn Arg His Leu Asn Asp Ser Arg Tyr Ile Ser 
945                 950                 955                 960 


Lys Val Val Lys Thr Leu Leu Ser Asn Ile Val Arg Thr Glu Asp Glu 
                965                 970                 975     


Met Glu Thr Thr Ser Lys Phe Val Val Ser Cys Thr Gly Gly Ile Thr 
            980                 985                 990         


Asp Arg Leu Lys Lys Asp Trp Gly  Leu Asn Asp Val Trp  Asn Ser Ile 
        995                 1000                 1005             


Val Tyr  Ser Arg Phe Glu Arg  Leu Asn Glu Leu Thr  Lys Ser Asp 
    1010                 1015                 1020             


Ala Phe  Gly Arg Trp Glu Asn  Lys Glu Gly Lys Arg  Val Phe Gln 
    1025                 1030                 1035             


Thr Ser  Val Pro Leu Glu Leu  Gln Arg Gly Phe Asn  Lys Lys Arg 
    1040                 1045                 1050             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1055                 1060                 1065             


Ala Ser  Arg Asn Ile Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Asn 
    1070                 1075                 1080             


Ser Pro  Lys Lys Arg Glu Asp  Leu Arg Gln Gln Leu  Cys Asp Lys 
    1085                 1090                 1095             


Asn Arg  Ile Ile Arg Lys Pro  Trp Glu Ser Phe Thr  Gln Asp Ala 
    1100                 1105                 1110             


Arg Ala  Ala Leu Glu Asp Val  Val Val Ser Phe Lys  Asn Tyr Val 
    1115                 1120                 1125             


Arg Val  Ile Asn Lys Ala Thr  Asn Tyr Tyr Glu Arg  Tyr Asp Ala 
    1130                 1135                 1140             


Asp Gly  Lys Lys Thr Ile Thr  Lys Gln Lys Ser Arg  Glu Met Trp 
    1145                 1150                 1155             


Ala Val  Arg Lys Pro Met His  Lys Glu Thr Val Phe  Gly His Val 
    1160                 1165                 1170             


Asn Leu  Arg Arg Lys Ala Ile  Val Lys Leu Lys Asp  Ala Leu Glu 
    1175                 1180                 1185             


Asn Ile  Pro Ser Ile Cys Asn  Lys Glu Leu Arg Asp  Tyr Ile Asn 
    1190                 1195                 1200             


Gly Leu  Val Glu Arg Asn Phe  Asn Thr Lys Gln Leu  His Ala His 
    1205                 1210                 1215             


Phe Arg  Ser Thr Asp Tyr Arg  Trp Asn Lys Gln Asn  Val Asp Lys 
    1220                 1225                 1230             


Val Glu  Val Trp Arg Phe Ser  Asp Glu Lys Glu Pro  Leu Val Ala 
    1235                 1240                 1245             


Thr Arg  Lys Pro Leu Asp Thr  Ser Phe Asp Ala Lys  Arg Ile Asp 
    1250                 1255                 1260             


Thr Ile  Thr Asp Thr Gly Ile  Gln Lys Ile Leu Leu  Asn Tyr Leu 
    1265                 1270                 1275             


Glu Ala  Lys Gly Gly Asp Pro  Leu Ile Ala Phe Thr  Pro Glu Gly 
    1280                 1285                 1290             


Ile Ala  Glu Met Asn Gln Asn  Ile Ala Leu Tyr Asn  Asp Gly Lys 
    1295                 1300                 1305             


Met His  Gln Pro Ile Leu Lys  Val Arg Ile Ser Glu  Pro Lys Gly 
    1310                 1315                 1320             


Ala Lys  Tyr Gln Ile Gly Gln  Thr Gly Asn Lys Ala  Asp Lys Phe 
    1325                 1330                 1335             


Val Glu  Ala Gln Lys Gly Thr  Asn Leu Tyr Phe Ala  Ile Tyr Glu 
    1340                 1345                 1350             


Asp Glu  Asp Arg Asn Arg Thr  Tyr Asn Thr Ile Pro  Leu Asn Glu 
    1355                 1360                 1365             


Val Ala  Glu Arg Leu Lys Gln  Gly Leu Pro Pro Val  Pro Glu Lys 
    1370                 1375                 1380             


Asn Glu  Lys Glu Val Pro Leu  Lys Phe Tyr Leu Ser  Pro Asn Asp 
    1385                 1390                 1395             


Leu Val  Tyr Val Pro Thr Glu  Glu Glu Ile Leu Met  Lys Glu Cys 
    1400                 1405                 1410             


Lys Leu  Asn Lys Asn Arg Ile  Tyr Lys Met Val Ser  Ser Thr Gly 
    1415                 1420                 1425             


Asn Gln  Cys Phe Phe Ile Pro  Phe Arg Val Ala Val  Ser Ile Val 
    1430                 1435                 1440             


Asn Lys  Met Glu Tyr Ser Pro  Leu Asn Lys Met Glu  Lys Ala Ile 
    1445                 1450                 1455             


Ser Ser  Glu Met Ile Lys Ala  Val Cys Trp Lys Leu  Glu Val Asp 
    1460                 1465                 1470             


Arg Leu  Gly Asn Ile Ile Arg  Val Val Lys 
    1475                 1480             


<210>  1286
<211>  1483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1286

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Val Ser Leu Asp Asp Asn Gly Asn Tyr Leu His Asp Ile Lys 
            20                  25                  30          


Leu Gly Cys Arg Ile Ile Pro Met Ser Gln Asp Val Leu Asp Asn Phe 
        35                  40                  45              


Gly Lys Gly Val Thr Val Ser Gln Thr Ser Val Arg Thr Gly Phe Arg 
    50                  55                  60                  


Gly Val Arg Arg Ile Arg Glu Arg Ser Leu Gln Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Arg Leu Gly Phe Leu Pro Gln His Tyr Asp Ala 
                85                  90                  95      


Ala Ile Gly Trp Asp Ser Asp Cys Ala Lys Thr Tyr Gly Lys Phe Leu 
            100                 105                 110         


Asp Asn Gly Glu Pro Lys Leu Ala Trp Glu Arg Gln Glu Asp Gly Ser 
        115                 120                 125             


Met Arg Phe Leu Phe Met Asn Ser Phe Tyr Glu Met Ile Ala Asp Phe 
    130                 135                 140                 


Thr Ser His Gln Pro Ala Leu Val Ala Asp Gly Lys Lys Ile Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Ala Ile 
                165                 170                 175     


Arg Lys Glu Glu Leu Ala Trp Ile Leu Leu Ser Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Asp Glu Asn Pro Thr Lys 
        195                 200                 205             


Arg Glu Glu Phe Cys Glu Leu Lys Val Val Ala Val Glu Ala Ala Glu 
    210                 215                 220                 


Lys Gly Lys Gly Asp Asp Ile Trp Tyr Asn Val His Leu Glu Asn Gly 
225                 230                 235                 240 


Cys Ile Tyr Arg Arg Ser Ser Lys Ile Ser Leu Asp Asp Trp Val Gly 
                245                 250                 255     


Lys Thr Lys Gln Phe Ile Ile Thr Thr Glu Tyr Glu Lys Asp Gly Val 
            260                 265                 270         


Thr Pro Lys Lys Asp Lys Asp Gly Lys Glu Lys Arg Ser Tyr Arg Ala 
        275                 280                 285             


Pro Lys Glu Asp Asp Trp Gly Leu Arg Lys Lys Arg Thr Glu Thr Asp 
    290                 295                 300                 


Leu Glu Ala Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Glu His Leu 
305                 310                 315                 320 


Leu Ala Glu Pro Phe Asp Lys Ile Arg Gly Asn Phe Ile His Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Arg Ala Glu Leu Glu Ala Ile Leu Thr Glu Gln 
            340                 345                 350         


Arg Lys Tyr His Glu Glu Leu Arg Ser Glu Gln Met Leu Ala Val Cys 
        355                 360                 365             


Ala Glu Glu Leu Tyr Arg Ser Asn Leu Pro His Gln Gln Ser Leu Leu 
    370                 375                 380                 


Lys Lys Asp Met Val Tyr Leu Leu Leu Ser Asp Leu Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asp Cys Pro Tyr Glu 
                405                 410                 415     


Tyr Tyr Arg Tyr Val Asp Lys Glu Thr Gly Glu Ile Lys Lys Gln Gly 
            420                 425                 430         


Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Val Leu Asn Leu Arg Leu Phe Asp Arg Met Thr Asp Lys 
    450                 455                 460                 


Glu Val Thr Ala Asp Tyr Leu Pro Thr Lys Glu Asp Tyr Val Arg Leu 
465                 470                 475                 480 


Phe Asp Tyr Leu Asn Asn Arg Lys Glu Ile Asn Gln Glu Thr Leu Leu 
                485                 490                 495     


Lys Asp Phe Phe Arg Leu Lys Lys Val Lys Leu Gly Arg Glu Lys Glu 
            500                 505                 510         


Phe Glu Ile Arg Trp Asn Tyr Ile Glu Asp Lys Glu Lys Lys Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg Tyr Glu Leu Leu Val Ala Leu Asp Arg Ala Gly 
    530                 535                 540                 


Ile Asp Arg Ser Trp Ile Asp Glu Asp Gly Arg Gln Tyr Arg Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Ile Glu Val Lys Ala Glu Ala Glu Ser Ala Leu 
                565                 570                 575     


Arg Lys Leu Asn Ser Asp Asp Ala Phe Val Ser Ser Phe Leu Lys Val 
            580                 585                 590         


Lys Pro Phe Lys Lys Asp Tyr Gly Ala Tyr Ser Glu Lys Ala Val Lys 
        595                 600                 605             


Lys Leu Leu Ala Val Met Arg Met Gly Ser Met Trp Ser Glu Ala Asn 
    610                 615                 620                 


Ile Cys Glu Glu Thr Gln Asn Arg Ile Arg Ser Ile Ile Arg Gly Asp 
625                 630                 635                 640 


Ile Glu Glu Lys Ile Lys Glu Arg Met Gly His Thr Ala Arg Ser Phe 
                645                 650                 655     


Asn Gln Ile Asp Asp Phe Gln Ala Leu Pro Glu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Val Val Tyr Gly Arg His Ser Glu Val Thr Asp Ile Gln His Trp Glu 
        675                 680                 685             


Thr Pro Glu Gln Leu Gln Ser Tyr Ile Asn Gly Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Cys Ile Leu Glu Thr Leu Arg Thr 
705                 710                 715                 720 


Val His Asp Ile Trp Lys Glu Ala Gly His Ile Asp Glu Ile His Val 
                725                 730                 735     


Glu Leu Gly Arg Asp Met Lys Arg Thr Ala Asp Gln Arg Ala Arg Met 
            740                 745                 750         


Thr Lys Asn Ile Leu Lys Asn Glu Asn Thr Asn Leu Arg Ile Lys Ser 
        755                 760                 765             


Leu Leu Met Glu Leu Lys Lys Asp Thr Asp Ile Lys Glu Val Arg Pro 
    770                 775                 780                 


Tyr Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala 
785                 790                 795                 800 


Leu Leu Gln Leu Lys Arg Asp Asp Glu Ala Phe Ala Asp Ile Ser Lys 
                805                 810                 815     


Ile Ser Gln Met Ala Gln Pro Thr Ala Thr Glu Leu Arg Arg Tyr Lys 
            820                 825                 830         


Leu Trp Leu Asp Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Phe Ile 
        835                 840                 845             


Ser Leu Ser Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Val Ile 
    850                 855                 860                 


Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val Ile Cys 
865                 870                 875                 880 


Glu Ala Glu Val Asn Arg Leu Lys Ser Asn Met Leu Gly Tyr Glu Phe 
                885                 890                 895     


Ile Lys Ala His Gly Gly Glu Ile Val His Cys Thr Met Leu Gly Asp 
            900                 905                 910         


Val Lys Ile Leu Ser Glu Ser Glu Tyr Lys Ala Phe Val Asn Glu His 
        915                 920                 925             


Tyr Val Asn His Arg Ala Lys Lys Glu Lys Leu Leu Met Glu Asp Ile 
    930                 935                 940                 


Pro Gln Glu Phe Leu Asn Arg Gln Met Asn Asp Ser Arg Tyr Ile Ser 
945                 950                 955                 960 


Lys Val Val Lys Ala Leu Leu Ser Asn Ile Val Arg Thr Glu Asp Glu 
                965                 970                 975     


Met Asp Val Thr Ser Lys Phe Val Val Pro Cys Ser Gly Gly Ile Thr 
            980                 985                 990         


Asp Arg Leu Lys Lys Asp Trp Gly  Leu Asn Asp Val Trp  Asn Ser Ile 
        995                 1000                 1005             


Val Tyr  Pro Arg Phe Glu Arg  Leu Asn Arg Met Thr  Gly Thr Glu 
    1010                 1015                 1020             


Ala Phe  Gly His Trp Glu Asn  Lys Glu Gly Lys Arg  Val Phe Gln 
    1025                 1030                 1035             


Thr Ser  Val Pro Ile Glu Leu  Gln Leu Gly Phe Ser  Lys Lys Arg 
    1040                 1045                 1050             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1055                 1060                 1065             


Ala Ser  Arg Asn Ile Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Asn 
    1070                 1075                 1080             


Ser Gln  Lys Lys Arg Glu Asp  Leu Arg Arg Leu Leu  Cys Asp Lys 
    1085                 1090                 1095             


Asn Arg  Ile Ile Arg Lys Pro  Trp Glu Ser Phe Thr  Gln Asp Ala 
    1100                 1105                 1110             


Arg Val  Ala Leu Gln Asp Ile  Val Val Ser Phe Lys  Asn Tyr Val 
    1115                 1120                 1125             


Arg Ile  Ile Asn Lys Ala Thr  Asn Tyr Tyr Glu Arg  Tyr Asp Glu 
    1130                 1135                 1140             


Gln Gly  Lys Lys Thr Thr Ala  Glu Gln Lys Ser Ser  Glu Met Trp 
    1145                 1150                 1155             


Ala Ile  Arg Lys Pro Met His  Lys Glu Thr Val Phe  Gly His Val 
    1160                 1165                 1170             


Asn Leu  Lys Arg Lys Thr Thr  Val Lys Leu Lys Asn  Ala Ile Glu 
    1175                 1180                 1185             


Asn Ile  Pro Asn Ile Cys Asn  Lys Glu Leu Lys Ala  Tyr Ile Asn 
    1190                 1195                 1200             


Glu Leu  Val Glu Lys His Phe  Asn Thr Lys Gln Met  Leu Ala His 
    1205                 1210                 1215             


Phe Lys  Ser Ile Asn Tyr Arg  Trp Asn Arg Gln Asn  Val Glu Lys 
    1220                 1225                 1230             


Val Glu  Val Trp Gln Tyr Ser  Asp Glu Lys Glu Arg  Met Ala Ala 
    1235                 1240                 1245             


Thr Arg  Lys Pro Leu Asp Thr  Ser Phe Asp Lys Lys  Arg Ile Glu 
    1250                 1255                 1260             


Thr Ile  Thr Asp Thr Gly Ile  Gln Lys Ile Leu Leu  Asn Tyr Leu 
    1265                 1270                 1275             


Glu Glu  Lys Gly Gly Asp Pro  Asn Ile Ala Phe Thr  Pro Glu Gly 
    1280                 1285                 1290             


Ile Ala  Glu Met Asn Lys Asn  Ile Ser Leu Phe Asn  Asp Gly Lys 
    1295                 1300                 1305             


Leu His  Gln Pro Ile Leu Lys  Val Arg Val Ala Lys  Thr Met Gly 
    1310                 1315                 1320             


Ala Lys  Tyr Gln Val Gly Gln  Thr Gly Asn Lys Ala  Ala Lys Tyr 
    1325                 1330                 1335             


Val Gly  Ala Gln Glu Gly Thr  Asn Leu Tyr Phe Ala  Ile Tyr Glu 
    1340                 1345                 1350             


Asn Glu  Glu Gly Glu Arg Ser  Tyr Asn Ala Val Pro  Leu Asn Glu 
    1355                 1360                 1365             


Val Ala  Glu Arg Leu Lys Gln  Gly Met Ser Pro Val  Pro Glu Lys 
    1370                 1375                 1380             


Asn Glu  Lys Asp Val Pro Leu  Lys Phe Tyr Leu Ser  Pro Asn Asp 
    1385                 1390                 1395             


Leu Val  Tyr Val Pro Thr Glu  Glu Asp Lys Leu Ser  Asn Glu Cys 
    1400                 1405                 1410             


Lys Leu  Asp Asn Glu Arg Ile  Tyr Lys Met Val Ser  Ala Thr Gly 
    1415                 1420                 1425             


Lys Lys  Cys His Phe Leu Pro  Tyr Ser Val Ala Asn  Ile Ile Tyr 
    1430                 1435                 1440             


Asn Lys  Val Glu Phe Glu Ser  Met Asn Lys Met Gly  Lys Ala Leu 
    1445                 1450                 1455             


Ser Gly  Glu Met Ile Lys Ala  Val Cys Trp Lys Leu  Glu Val Asp 
    1460                 1465                 1470             


Arg Leu  Gly Thr Ile Leu Arg  Val Ile Lys 
    1475                 1480             


<210>  1287
<211>  1483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1287

Met Lys Thr Glu Leu Asp Leu Asn Lys Asp Arg Leu Tyr Lys His Val 
1               5                   10                  15      


Leu Gly Leu Asp Leu Gly Val Thr Ser Ile Gly Phe Ala His Val Val 
            20                  25                  30          


Glu Ser Glu Asn Arg Asn Glu Ser Glu Ile Lys Gln Ile Gly Val Arg 
        35                  40                  45              


Val Ile Gln Leu Asp Asn Phe Asp Lys Val Asp Lys Ser Gly Lys Thr 
    50                  55                  60                  


Ser Glu Ser Lys Asn Pro Ala Val Asp Phe Ala Ala Gly Lys Gly Leu 
65                  70                  75                  80  


Thr Pro Asn Gly Ser Arg Thr Lys Lys Arg Gly Ala Arg Arg Asn Leu 
                85                  90                  95      


Asp Arg Tyr Gln Asp Arg Arg Asp Asn Leu Leu Glu Val Leu Thr Lys 
            100                 105                 110         


Ser Gly Phe Ile Asn Ser Glu Thr Lys Arg Thr Glu Asp Gly Lys His 
        115                 120                 125             


Thr Thr His Gln Thr Trp Glu Ile Arg Ala Lys Ala Val Ser Glu Lys 
    130                 135                 140                 


Val Glu Lys Glu Glu Phe Ala Arg Ile Leu Leu Ala Ile Asn Lys Lys 
145                 150                 155                 160 


Arg Gly Tyr Lys Ser Ser Arg Lys Ala Lys Asn Glu Asp Glu Gly Gln 
                165                 170                 175     


Ala Val Asp Gly Met Ala Val Ala Lys Arg Leu Tyr Glu Glu Asn Leu 
            180                 185                 190         


Thr Pro Gly Gln Tyr Ala Tyr Glu Val Leu Ser Gly Gly Lys Lys Ser 
        195                 200                 205             


Ile Pro Asp Phe Tyr Arg Ser Asp Leu Gln Ala Glu Phe Asp Lys Ile 
    210                 215                 220                 


Trp Asn Phe Gln Lys Gln Phe Tyr Pro Asn Ile Leu Thr Asp Glu Phe 
225                 230                 235                 240 


Tyr Glu Glu Leu Lys Gly Lys Gly Gln Arg Ala Thr Ser Ala Ala Phe 
                245                 250                 255     


Trp Lys Ile His Gln Phe Asn Thr Ala Asp Ile Lys Asp Leu Glu Asp 
            260                 265                 270         


Glu Leu Lys Asn Glu Arg Thr Ile Lys Leu Ser Lys Arg Asp Gln Lys 
        275                 280                 285             


Lys Leu Gln Ala Tyr Lys Trp Arg Ser Glu Ala Val Val Lys Gln Leu 
    290                 295                 300                 


Gly Lys Glu Glu Val Ala Tyr Val Leu Thr Glu Ile Asn Asn Asn Leu 
305                 310                 315                 320 


Asn Asn Ser Ser Gly Tyr Leu Gly Ala Ile Ser Asp Arg Ser Lys Glu 
                325                 330                 335     


Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln Phe Leu Tyr Lys Gln Leu 
            340                 345                 350         


Lys Glu Asn Pro His Thr Arg Leu Lys Asn Gln Val Phe Tyr Arg Gln 
        355                 360                 365             


Asp Tyr Leu Asp Glu Phe Glu Thr Ile Trp Glu Met Gln Ala Gln Phe 
    370                 375                 380                 


His Pro Glu Leu Thr Gln Glu Leu Lys Glu Glu Ile Arg Asp Ile Ile 
385                 390                 395                 400 


Ile Phe Tyr Gln Arg Lys Leu Lys Ser Gln Lys Gly Leu Ile Ser Phe 
                405                 410                 415     


Cys Glu Leu Glu Ser Lys Glu Val Val Ile Asn Asp Thr Lys Lys Ile 
            420                 425                 430         


Ile Gly Leu Arg Val Ala Pro Lys Ser Ser Pro Leu Phe Gln Glu Phe 
        435                 440                 445             


Lys Ile Trp Gln Asn Leu Asn Asn Val Leu Val Arg Lys Lys Gly Ser 
    450                 455                 460                 


Arg Lys Arg Met Val Lys Ala Gln Asn Gln His Ser Leu Phe Glu Glu 
465                 470                 475                 480 


Pro Lys Glu Ile Phe Ser Leu Asp Leu Ala Ser Lys Glu Lys Leu Phe 
                485                 490                 495     


Glu Glu Leu Asn Leu Lys Gly Asn Leu Ser Ala Lys Asn Cys Leu Asp 
            500                 505                 510         


Ile Leu Gly Leu Asn Ser Asn Glu Trp Glu Leu Asn Tyr Thr Gln Leu 
        515                 520                 525             


Glu Gly Asn Arg Thr Asn Lys Ser Leu Tyr Glu Ala Phe Leu Lys Ile 
    530                 535                 540                 


Leu Asp Ile Glu Gly Tyr Asp Val Lys Asp Leu Leu Lys Val Lys Ser 
545                 550                 555                 560 


Ser Lys Asp Glu Val Glu Leu Asp Asp Leu Lys Val Pro Ala Ser Glu 
                565                 570                 575     


Ile Lys Asn Met Val Arg Glu Ile Phe Ser Thr Asn Lys Ile Asp Thr 
            580                 585                 590         


Glu Ile Leu Asp Phe Asp Ala Glu Ile Asp Gly Lys Ala Phe Glu Lys 
        595                 600                 605             


Gln Ser Ser Tyr Gln Leu Trp His Leu Leu Tyr Ser Tyr Glu Gly Asp 
    610                 615                 620                 


Asp Ser Gln Ser Gly Asn Glu Lys Leu Tyr Glu Leu Leu Ala Lys Lys 
625                 630                 635                 640 


Phe Gly Phe Lys Arg Glu His Ala Gln Ile Leu Ala Asn Val Ser Leu 
                645                 650                 655     


Val Asp Asp Tyr Gly Asn Leu Ser Ser Lys Ala Met Arg Lys Ile Tyr 
            660                 665                 670         


Pro His Ile Arg Glu Asn Asn Tyr Ser Thr Ala Cys Glu Leu Val Gly 
        675                 680                 685             


Tyr Arg His Ser Lys His Ser Leu Thr Lys Asp Glu Leu Lys Asp Arg 
    690                 695                 700                 


Val Leu Glu Asp Arg Leu Glu Leu Leu Lys Lys Asn Ser Leu Arg Gln 
705                 710                 715                 720 


Pro Val Val Glu Lys Ile Leu Asn Gln Met Ile Asn Ala Val Asn Ala 
                725                 730                 735     


Leu Ile Glu Lys Asn Ser Glu Arg Asp Glu Asn Gly Asn Ile Thr Lys 
            740                 745                 750         


Tyr Phe Lys Phe Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys 
        755                 760                 765             


Lys Asn Ala Ala Glu Arg Ala Glu Met Thr Thr Asn Ile Asn Ala Ala 
    770                 775                 780                 


Lys Leu Val His Glu Lys Ile Phe Lys Ile Leu Gln Asn Glu Phe Gly 
785                 790                 795                 800 


Ile Lys Asn Pro Thr Arg Asn Asp Ile Ile Arg Tyr Arg Leu Tyr Glu 
                805                 810                 815     


Glu Leu Lys Gly Asn Gly Tyr Lys Asp Leu Tyr Ser Asn Thr Tyr Ile 
            820                 825                 830         


Pro Arg Glu Ile Leu Phe Ser Asn Gln Ile Asp Ile Asp His Ile Leu 
        835                 840                 845             


Pro Gln Ser Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Val Val Val 
    850                 855                 860                 


Phe Arg Lys Asp Asn Leu Lys Lys Gly Asn Gln Thr Ala Ile Asp Tyr 
865                 870                 875                 880 


Ile Gly Ser Glu Val Gly Glu Glu Gly Leu Lys Asn Phe Ile Glu Arg 
                885                 890                 895     


Val Glu Ser Leu Tyr Glu Leu Gly Lys Lys Asn Lys Glu Glu Gly Ile 
            900                 905                 910         


Ser Lys Ala Lys Tyr Gln Lys Leu Leu Lys Ser Ala Ser Glu Ile Gly 
        915                 920                 925             


Asp Gly Phe Ile Glu Arg Asp Leu Arg Glu Ser Gln Tyr Ile Ala Lys 
    930                 935                 940                 


Lys Ala Lys Glu Leu Leu Phe Lys Val Thr Arg Ser Val Val Ser Thr 
945                 950                 955                 960 


Ser Gly Ser Ile Thr Asp Arg Leu Arg Glu Asp Trp Gly Leu Ile Asn 
                965                 970                 975     


Val Met Lys Glu Leu Asn Leu Pro Lys Tyr Lys Ser Leu Gly Leu Thr 
            980                 985                 990         


Glu Ile Gln Glu Arg Arg Asn Gly  His Gln Val Glu Val  Ile Lys Asp 
        995                 1000                 1005             


Trp Thr  Lys Arg Asn Asp His  Arg His His Ala Met  Asp Ala Leu 
    1010                 1015                 1020             


Ala Val  Ala Phe Thr Lys His  Asn His Ile Gln Tyr  Leu Asn Tyr 
    1025                 1030                 1035             


Leu Asn  Ala Arg Lys Asn Glu  Ser His Lys Leu His  Gly Asn Ile 
    1040                 1045                 1050             


Ile Ala  Ile Glu Thr Leu Glu  Thr Glu Val Lys Ile  Asp Asp Leu 
    1055                 1060                 1065             


Gly Asn  Lys Lys Arg Val Phe  Lys Glu Pro Ile Pro  Asn Phe Arg 
    1070                 1075                 1080             


Gln Val  Ala Lys Glu His Leu  Glu Asn Val Leu Ile  Ser His Lys 
    1085                 1090                 1095             


Ala Lys  Asn Lys Val Val Thr  Lys Asn Lys Asn Lys  Ile Ser Gly 
    1100                 1105                 1110             


Lys Glu  Lys Leu Gln Ile Thr  Leu Thr Pro Arg Gly  Gln Leu His 
    1115                 1120                 1125             


Lys Glu  Thr Val Tyr Gly Lys  Tyr Arg Phe Tyr Gln  Ser Lys Asp 
    1130                 1135                 1140             


Glu Lys  Val Ser Ala Lys Phe  Asp Leu Glu Thr Ile  Glu Lys Val 
    1145                 1150                 1155             


Ala Asn  Pro Ser Tyr Lys Ala  Ala Leu Leu Gln Arg  Leu Asn Glu 
    1160                 1165                 1170             


Asn Glu  Asn Asp Pro Lys Lys  Ala Phe Ser Gly Lys  Asn Ala Leu 
    1175                 1180                 1185             


Ser Lys  Asn Pro Ile Tyr Ile  Ser Met Glu Asn Asp  Ile Lys Val 
    1190                 1195                 1200             


Pro Asp  Thr Val Lys Leu Val  Trp Leu Glu Glu Asp  Tyr Ser Ile 
    1205                 1210                 1215             


Arg Lys  Asp Val Thr Pro Glu  Asn Phe Lys Asp Glu  Lys Ser Ile 
    1220                 1225                 1230             


Glu Lys  Ile Leu Asp Glu Gly  Val Lys Arg Ile Leu  Lys Asn Arg 
    1235                 1240                 1245             


Leu Lys  Glu Phe Gly Gly Asp  Ala Lys Glu Ala Phe  Ser Asp Leu 
    1250                 1255                 1260             


Asp Lys  Asn Pro Ile Trp Leu  Asn Glu Glu Lys Gly  Thr Ser Ile 
    1265                 1270                 1275             


Lys Arg  Val Arg Ile Ser Gly  Val Lys Asn Ala Glu  Pro Leu His 
    1280                 1285                 1290             


Tyr Lys  Lys Asp His Phe Gly  Asn Asp Ile Leu Asp  Gly Asn Gly 
    1295                 1300                 1305             


Asn Lys  Ile Pro Ala Asp Phe  Val Ser Thr Gly Asn  Asn His His 
    1310                 1315                 1320             


Val Ala  Ile Tyr Lys Glu Pro  Glu Phe Asp Lys Lys  Gly Gln Pro 
    1325                 1330                 1335             


Val Leu  Asp Glu Leu Gly Asn  Gln Lys Tyr Asn Leu  Gln Glu Lys 
    1340                 1345                 1350             


Val Val  Ser Phe Phe Glu Ala  Val Glu Arg Val Asn  Gln Gly Leu 
    1355                 1360                 1365             


Pro Ile  Ile Asp Lys Thr Tyr  Asn Gln His Leu Gly  Trp Gln Phe 
    1370                 1375                 1380             


Phe Phe  Thr Met Lys Gln Asn  Glu Met Phe Val Phe  Pro Asn Glu 
    1385                 1390                 1395             


Lys Thr  Arg Phe Asn Pro Ile  Glu Ile Asp Leu Leu  Asp Pro Asn 
    1400                 1405                 1410             


Asn Lys  Lys Leu Ile Ser Leu  Asn Leu Phe Arg Val  Gln Lys Ile 
    1415                 1420                 1425             


Ala Asn  Lys Asn Tyr Met Phe  Arg His His Leu Glu  Thr Asn Val 
    1430                 1435                 1440             


Asp Asp  Lys Lys Glu Met Lys  Asn Ile Ala Phe Ile  His Ile Gln 
    1445                 1450                 1455             


Ser Cys  Asn Leu Leu Lys Glu  Ile Ile Lys Val Arg  Leu Asn His 
    1460                 1465                 1470             


Leu Gly  Asp Ile Val Ser Val  Gly Glu Tyr 
    1475                 1480             


<210>  1288
<211>  1483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1288

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Val Ser Val Asp Asp His Gly Asn Tyr Leu His Asp Ile Lys 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Asn Phe 
        35                  40                  45              


Asp Lys Gly Val Thr Glu Ser Gln Thr Ala Ile Arg Thr Gly Phe Arg 
    50                  55                  60                  


Ser Val Arg Arg Ile Arg Glu Arg Ser Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Val Met Gly Phe Leu Pro Pro His Tyr Asp Ser 
                85                  90                  95      


Ala Ile Gly Trp Asn Arg Glu Glu Ala Ser Thr Tyr Gly Lys Phe Leu 
            100                 105                 110         


Asn His Gly Glu Pro Lys Leu Ala Trp Glu Arg Leu Glu Asp Gly Ser 
        115                 120                 125             


Met Arg Phe Leu Phe Met Asp Ser Phe His Glu Met Met Ala Asn Phe 
    130                 135                 140                 


Ala Arg His Gln Pro Ala Leu Val Ala Asp Gly Arg Lys Ile Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Ala Val 
                165                 170                 175     


Gly Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Cys Gln Leu Arg Gly Glu Glu Glu Glu Glu Thr Pro Thr Lys 
        195                 200                 205             


Arg Glu Glu Tyr Tyr Glu Leu Lys Val Val Ser Val Glu Ala Ala Glu 
    210                 215                 220                 


Glu Gly Lys Gly Gly Asn Thr Trp Tyr Arg Val Gln Leu Glu Asn Gly 
225                 230                 235                 240 


Trp Ile Tyr Arg Arg Pro Ser Lys Ile Ala Leu Asp Asp Trp Val Gly 
                245                 250                 255     


Lys Thr Lys Gln Phe Ile Val Thr Thr Glu Tyr Glu Lys Asp Gly Thr 
            260                 265                 270         


Thr Leu Lys Lys Asp Lys Asp Gly Glu Val Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Pro Gly Glu Asn Asp Trp Gly Leu Arg Lys Lys Arg Thr Glu Ser Gln 
    290                 295                 300                 


Leu Glu Ala Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Asp His Leu 
305                 310                 315                 320 


Leu Ala Glu Pro Ala Asp Lys Ile Arg Gly Asn Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Gln Glu Leu Glu Ala Ile Leu Arg Glu Gln 
            340                 345                 350         


Thr Lys His His Glu Glu Leu Arg Ser Lys Glu Met Leu Ala Lys Cys 
        355                 360                 365             


Ala Glu Glu Leu Tyr Gln Ser Asn Gln Pro His Arg Lys Ser Leu Leu 
    370                 375                 380                 


Asn Lys Asp Met Thr Tyr Leu Leu Leu Thr Asp Leu Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Glu Cys Pro Tyr Glu 
                405                 410                 415     


Tyr Tyr Lys Tyr Lys Asp Lys Glu Lys Gly Lys Lys Lys Arg Gln Gly 
            420                 425                 430         


Ile Lys Cys Val Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Ile Val Asn Leu Arg Leu Phe Asp Arg Thr Asp Asp Lys 
    450                 455                 460                 


Glu Val Thr Ala Glu Tyr Leu Arg Ser Arg Glu Asp Tyr Ala Arg Leu 
465                 470                 475                 480 


Phe Ala Tyr Leu Asn Asp Arg Lys Glu Ile Asn Gln Glu Thr Leu Leu 
                485                 490                 495     


Lys Asp Phe Phe Gly Leu Lys Lys Val Lys Ile Gly Ala Glu Lys Glu 
            500                 505                 510         


Phe Ala Ile Arg Trp Asn Tyr Ile Glu Asp Lys Glu Lys Lys Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg His Glu Leu Leu Leu Ala Leu Asp Arg Ala Gly 
    530                 535                 540                 


Met Asp Arg Ser Trp Leu Asp Asp Ser Glu Leu Gln Tyr Arg Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Val Glu Ala Arg Asp Glu Ala Glu Gly Ala Leu 
                565                 570                 575     


Arg Lys Leu Arg Asp Asp Asp Glu Phe Val Gly Ala Phe Leu Lys Val 
            580                 585                 590         


Lys Pro Phe Lys Lys Asp Tyr Gly Ala Tyr Ser Glu Lys Ala Val Lys 
        595                 600                 605             


Lys Leu Leu Ala Val Met Arg Met Gly Ser Trp Trp His Glu Glu Asp 
    610                 615                 620                 


Ile Cys Lys Gln Thr Arg Asp Arg Ile Glu Arg Ile Val Gln Gly Asp 
625                 630                 635                 640 


Ile Glu Glu Lys Ile Arg Glu Arg Met Gly His Ala Ala Tyr Ser Phe 
                645                 650                 655     


Ser Arg Leu Ala Asp Phe Gln Gly Leu Pro Glu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Val Val Tyr Gly Arg His Ser Glu Ala Thr His Ile Leu Arg Trp Asp 
        675                 680                 685             


Ser Pro Asp Lys Leu Leu Ser Tyr Ile Lys Gly Phe Arg Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Cys Ile Leu Glu Thr Leu Arg Thr 
705                 710                 715                 720 


Val His Asp Ile Trp Lys Glu Ala Gly His Ile Asp Glu Ile His Val 
                725                 730                 735     


Glu Leu Gly Arg Ser Met Lys Ser Thr Ala Glu Gln Arg Ala His Met 
            740                 745                 750         


Thr Lys Asn Ile Leu Arg Asn Glu Asn Thr Asn Leu Arg Ile Lys Cys 
        755                 760                 765             


Leu Leu Met Glu Leu Lys Asn Asp Ala Asp Val Lys Asp Val Arg Pro 
    770                 775                 780                 


Tyr Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala 
785                 790                 795                 800 


Leu Gln Gln Leu Thr Lys Asp Asp Ala Glu Tyr Ala Glu Ile Ser Lys 
                805                 810                 815     


Ile Ser Lys Met Ala Gln Pro Ser Ala Lys Glu Leu Leu Arg Tyr Lys 
            820                 825                 830         


Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Pro Ile 
        835                 840                 845             


Ser Leu Ser Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Val Ile 
    850                 855                 860                 


Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Asn Asn Lys Val Ile Cys 
865                 870                 875                 880 


Glu Ser Glu Val Asn Thr Leu Lys Ser Asn Met Leu Gly Tyr Glu Phe 
                885                 890                 895     


Ile Lys Asn His Cys Gly Glu Ile Val His Cys Thr Met Leu Gly Asp 
            900                 905                 910         


Val Lys Val Phe Ser Glu Ser Glu Tyr Lys Ala Phe Val Thr Glu His 
        915                 920                 925             


Tyr Ser Asn Asn Lys Tyr Lys Lys Glu Lys Leu Leu Leu Glu Asp Ile 
    930                 935                 940                 


Pro Gln Glu Phe Leu Asn Arg Gln Met Asn Asp Ser Arg Tyr Ile Ser 
945                 950                 955                 960 


Lys Val Val Lys Gly Leu Leu Ser Asn Val Val Arg Thr Glu Asp Glu 
                965                 970                 975     


Leu Glu Ala Thr Ser Lys Phe Val Val Ser Cys Thr Gly Gly Ile Thr 
            980                 985                 990         


Asp Arg Leu Lys Lys Asp Trp Gly  Leu Asn Asp Val Trp  Asn Ser Ile 
        995                 1000                 1005             


Val Tyr  Ser Arg Phe Glu Arg  Leu Asn Gly Leu Thr  Lys Ser Glu 
    1010                 1015                 1020             


His Phe  Gly His Trp Glu Asn  Lys Glu Gly Lys Arg  Val Phe Gln 
    1025                 1030                 1035             


Thr Ser  Val Pro Met Glu Leu  Gln Arg Gly Phe Asn  Lys Lys Arg 
    1040                 1045                 1050             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1055                 1060                 1065             


Ala Ser  Arg Asn Ile Ile Asn  Tyr Leu Asn Asn Glu  Phe Ala Lys 
    1070                 1075                 1080             


Ser Pro  Lys Gln Arg Glu Asp  Leu Arg Gln Leu Leu  Cys Asp Lys 
    1085                 1090                 1095             


Asn Arg  Ile Ile Arg Lys Pro  Trp Glu Thr Phe Thr  Gln Asp Ala 
    1100                 1105                 1110             


Arg Ala  Ala Leu Glu Asp Val  Val Val Ser Phe Lys  Asn Tyr Val 
    1115                 1120                 1125             


Arg Val  Ile Asn Lys Thr Thr  Asn Tyr Tyr Glu Arg  Tyr Asp Ala 
    1130                 1135                 1140             


Asp Gly  Lys Lys Thr Met Val  Pro Gln Lys Gly Asn  Glu Met Trp 
    1145                 1150                 1155             


Ala Val  Arg Lys Pro Met His  Glu Gly Thr Met Tyr  Gly Arg Val 
    1160                 1165                 1170             


Asn Leu  Arg Arg Lys Ala Val  Val Lys Leu Lys Glu  Ala Leu Glu 
    1175                 1180                 1185             


Asn Ile  Ser Ser Ile Cys Asn  Lys Glu Leu Arg Asp  Tyr Ile Asn 
    1190                 1195                 1200             


Val Leu  Val Glu Lys Gln Phe  Asn Thr Lys Gln Leu  Leu Ala His 
    1205                 1210                 1215             


Phe Lys  Ser Met Asp Tyr Arg  Trp Asn Lys Gln His  Ile Asp Lys 
    1220                 1225                 1230             


Val Glu  Val Trp Arg Phe Ser  Asp Glu Lys Val Gln  Met Val Ala 
    1235                 1240                 1245             


Ala Arg  Lys Pro Leu Asp Thr  Ser Phe Asp Lys Glu  Cys Ile Ala 
    1250                 1255                 1260             


Ser Ile  Thr Asp Thr Gly Ile  Gln Lys Ile Leu Leu  Asn Tyr Leu 
    1265                 1270                 1275             


Glu Ser  Lys Gly Gly Asp Pro  Ala Ile Ala Phe Thr  Ser Glu Gly 
    1280                 1285                 1290             


Ile Ala  Glu Met Asn Gln Asn  Ile Ala Val Tyr Asn  Asp Gly Lys 
    1295                 1300                 1305             


Lys His  Gln Pro Val Ile Lys  Val Arg Lys Ser Thr  Lys Leu Gly 
    1310                 1315                 1320             


Lys Lys  Phe Ala Val Gly Gln  Ile Gly Cys Lys Ser  Lys Gln Phe 
    1325                 1330                 1335             


Val Val  Ala Glu Ala Gly Thr  Asn Leu Tyr Phe Ala  Ile Tyr Glu 
    1340                 1345                 1350             


Asp Glu  Glu Gly Lys Arg Ser  Tyr Asp Thr Ile Pro  Leu Asn Glu 
    1355                 1360                 1365             


Val Thr  Glu Arg Leu Lys Gln  Gly Leu Ser Pro Val  Pro Glu Lys 
    1370                 1375                 1380             


Asn Glu  Lys Glu Val Pro Leu  Lys Phe Tyr Leu Ser  Pro Asn Asp 
    1385                 1390                 1395             


Leu Val  Tyr Val Pro Thr Glu  Asp Glu Met Gln Met  Lys Glu Cys 
    1400                 1405                 1410             


Lys Leu  Asn Lys Glu Arg Ile  Tyr Lys Met Val Ser  Ser Thr Gly 
    1415                 1420                 1425             


Asn Glu  Cys His Phe Leu Pro  Tyr Ser Val Ala Ser  Val Ile Tyr 
    1430                 1435                 1440             


Asn Lys  Val Glu Phe Glu Ser  Met Asn Lys Met Gly  Arg Ala Leu 
    1445                 1450                 1455             


Thr Gly  Glu Met Ile Lys Ala  Val Cys Trp Lys Leu  Asp Val Asp 
    1460                 1465                 1470             


Arg Leu  Gly Asn Ile Leu Arg  Val Ile Lys 
    1475                 1480             


<210>  1289
<211>  1483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1289

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Ile Ser Ile Asp Asp Asn Gly Asn Tyr Leu Arg Asp Ile Gln 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly Asn Phe 
        35                  40                  45              


Asp Asn Gly Val Thr Glu Ser Gln Thr Ala Val Arg Thr Gly Phe Arg 
    50                  55                  60                  


Gly Leu Arg Arg Ile Arg Glu Arg Ser Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Val Met Gly Phe Leu Pro Pro His Tyr Asp Ser 
                85                  90                  95      


Ala Ile Gly Trp Asn Lys Glu Glu Pro Thr Thr Tyr Gly Lys Phe Leu 
            100                 105                 110         


Asn His Gly Glu Pro Lys Leu Ala Trp Glu Arg Gln Gly Asp Gly Ser 
        115                 120                 125             


Met Arg Phe Leu Phe Met Asp Ser Phe His Lys Met Met Glu Asp Phe 
    130                 135                 140                 


Ala Pro His Gln Pro Ala Leu Val Ala Asp Gly Arg Lys Ile Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Pro Ile 
                165                 170                 175     


Lys Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu Asn Pro Thr Lys 
        195                 200                 205             


Arg Glu Glu Tyr His Glu Leu Lys Val Val Gly Val Glu Ala Ala Glu 
    210                 215                 220                 


Glu Gly Lys Gly Gly Asn Thr Trp Tyr Asn Val Lys Leu Glu Asn Gly 
225                 230                 235                 240 


Trp Ile Tyr Arg Arg Ser Ser Lys Ile Pro Leu Asp Asp Trp Val Gly 
                245                 250                 255     


Lys Thr Arg Pro Phe Ile Val Thr Thr Glu Tyr Glu Ala Asp Gly Ile 
            260                 265                 270         


Thr Leu Lys Lys Asn Lys Glu Gly Glu Val Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Pro Gly Glu Asp Asp Trp Gly Leu Trp Lys Lys Arg Thr Glu Ser Gln 
    290                 295                 300                 


Leu Glu Ala Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Asp His Leu 
305                 310                 315                 320 


Leu Ala Glu Pro Ala Asp Lys Ile Arg Gly Asn Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Gln Glu Leu Ala Asp Ile Leu Arg Glu Gln 
            340                 345                 350         


Ser Lys Tyr His Glu Glu Leu Gln Ser Lys Lys Ile Leu Ala Asp Cys 
        355                 360                 365             


Ala Glu Glu Leu Tyr Arg Ser Asn Gln Pro His Arg Asp Ser Leu Leu 
    370                 375                 380                 


Lys Lys Asp Met Val Tyr Leu Leu Leu Thr Asp Leu Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asp Cys Pro Tyr Glu 
                405                 410                 415     


Tyr Tyr Glu Tyr Val Asp Lys Glu Thr Gly Glu Ile Lys Arg Gln Gly 
            420                 425                 430         


Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Val Leu Asn Leu Arg Leu Tyr Asp Arg Thr Glu Asp Lys 
    450                 455                 460                 


Glu Val Thr Ala Glu Tyr Leu Pro Ser Lys Glu Asp Tyr Ala Arg Leu 
465                 470                 475                 480 


Phe Val Tyr Leu Asn Asp Arg Lys Glu Ile Asn Gln Glu Thr Leu Leu 
                485                 490                 495     


Lys Asp Phe Phe Lys Leu Lys Lys Val Lys Gln Arg Ser Glu Lys Asp 
            500                 505                 510         


Phe Pro Val Arg Trp Asn Tyr Ile Glu Asp Lys Glu Lys Lys Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg His Glu Leu Leu Met Ala Leu Asp Arg Ala Gly 
    530                 535                 540                 


Ile Glu Arg Ser Trp Leu Asp Asp Thr Ala Arg Gln Tyr Arg Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Ile Glu Val Lys Asp Glu Ala Glu Arg Ala Leu 
                565                 570                 575     


Arg Lys Leu Lys Asp Asp Asp Glu Phe Val Ser Ala Phe Leu Lys Val 
            580                 585                 590         


Lys Pro Phe Lys Lys Asp Tyr Gly Ala Tyr Ser Glu Lys Ala Val Lys 
        595                 600                 605             


Lys Leu Leu Ala Val Met Arg Met Gly Ser His Trp Cys Glu Asp Asp 
    610                 615                 620                 


Ile Cys Lys Glu Thr Lys Glu Arg Ile Glu His Ile Leu Gln Gly Asp 
625                 630                 635                 640 


Val Gly Glu Lys Ile Lys Glu Arg Met Asn His Thr Ala Tyr Ser Leu 
                645                 650                 655     


Gly Glu Leu Ser Asp Phe Gln Ala Leu Pro Glu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Val Val Tyr Gly Arg His Ser Glu Ala Thr Asp Ile Lys His Trp Lys 
        675                 680                 685             


Thr Pro Glu Ser Leu Met Ala Tyr Ile Asn Gly Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Cys Ile Leu Glu Thr Leu Arg Thr 
705                 710                 715                 720 


Val His Asp Ile Trp Lys Glu Ala Gly His Ile Asp Glu Ile His Val 
                725                 730                 735     


Glu Leu Gly Arg Ser Met Lys Ser Thr Ser Asp Gln Arg Ala Arg Met 
            740                 745                 750         


Thr Glu Asn Ile Leu Arg Asn Glu Asn Thr Asn Leu Arg Ile Lys Ser 
        755                 760                 765             


Leu Leu Met Glu Leu Lys Asn Asp Ala Asp Ile Lys Asp Val Arg Pro 
    770                 775                 780                 


Tyr Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala 
785                 790                 795                 800 


Leu Gln Glu Leu Thr Lys Glu Asp Asn Asp Tyr Glu Glu Ile Phe Lys 
                805                 810                 815     


Ile Ser Lys Met Pro Gln Pro Ser Ala Lys Glu Leu Leu Lys Tyr Lys 
            820                 825                 830         


Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Pro Ile 
        835                 840                 845             


Ser Leu Ser Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Ile Ile 
    850                 855                 860                 


Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Asn Asn Lys Val Ile Cys 
865                 870                 875                 880 


Glu Ser Glu Val Asn Ala Leu Lys Gly Asn Met Leu Gly Tyr Glu Phe 
                885                 890                 895     


Ile Lys Asn His Gly Gly Glu Ile Val His Cys Thr Leu Leu Gly Asp 
            900                 905                 910         


Val Lys Val Gln Asn Glu Ser Glu Tyr Lys Ala Phe Val Thr Glu His 
        915                 920                 925             


Tyr Ala Asn Asn Lys Tyr Lys Lys Glu Lys Leu Leu Met Glu Asp Ile 
    930                 935                 940                 


Pro Gln Asp Phe Leu Asn Arg His Leu Asn Asp Ser Arg Tyr Ile Ser 
945                 950                 955                 960 


Lys Val Val Lys Thr Leu Leu Ser Asn Ile Val Arg Thr Glu Asp Glu 
                965                 970                 975     


Met Glu Thr Thr Ser Lys Phe Val Val Ser Cys Thr Gly Gly Ile Thr 
            980                 985                 990         


Asp Arg Leu Lys Lys Asp Trp Gly  Leu Asn Asp Val Trp  Asn Ser Ile 
        995                 1000                 1005             


Val Tyr  Ser Arg Phe Glu Arg  Leu Asn Glu Leu Thr  Lys Ser Asp 
    1010                 1015                 1020             


Ala Phe  Gly Arg Trp Glu Asn  Lys Glu Gly Lys Arg  Val Phe Gln 
    1025                 1030                 1035             


Thr Ser  Val Pro Leu Glu Leu  Gln Arg Gly Phe Asn  Lys Lys Arg 
    1040                 1045                 1050             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1055                 1060                 1065             


Ala Ser  Arg Asn Ile Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Asn 
    1070                 1075                 1080             


Ser Pro  Lys Lys Arg Glu Asp  Leu Arg Gln Gln Leu  Cys Asp Lys 
    1085                 1090                 1095             


Asn Arg  Ile Ile Arg Lys Pro  Trp Glu Ser Phe Thr  Gln Asp Ala 
    1100                 1105                 1110             


Arg Ala  Ala Leu Glu Asp Val  Val Val Ser Phe Lys  Asn Tyr Val 
    1115                 1120                 1125             


Arg Val  Ile Asn Lys Ala Thr  Asn Tyr Tyr Glu Arg  Tyr Asp Ala 
    1130                 1135                 1140             


Asp Gly  Lys Lys Thr Ile Thr  Lys Gln Lys Ser Arg  Glu Met Trp 
    1145                 1150                 1155             


Ala Val  Arg Lys Pro Met His  Lys Glu Thr Val Phe  Gly His Val 
    1160                 1165                 1170             


Asn Leu  Arg Arg Lys Ala Ile  Val Lys Leu Lys Asp  Ala Leu Glu 
    1175                 1180                 1185             


Asn Ile  Pro Ser Ile Cys Asn  Lys Glu Leu Arg Asp  Tyr Ile Asn 
    1190                 1195                 1200             


Gly Leu  Val Glu Arg Asn Phe  Asn Thr Lys Gln Leu  His Ala His 
    1205                 1210                 1215             


Phe Arg  Ser Thr Asp Tyr Arg  Trp Asn Lys Gln Asn  Val Asp Lys 
    1220                 1225                 1230             


Val Glu  Val Trp Arg Phe Ser  Asp Glu Lys Glu Pro  Leu Val Ala 
    1235                 1240                 1245             


Thr Arg  Lys Pro Leu Asp Thr  Ser Phe Asp Ala Lys  Arg Ile Asp 
    1250                 1255                 1260             


Thr Ile  Thr Asp Thr Gly Ile  Gln Lys Ile Leu Leu  Asn Tyr Leu 
    1265                 1270                 1275             


Glu Ala  Lys Gly Gly Asp Pro  Leu Ile Ala Phe Thr  Pro Glu Gly 
    1280                 1285                 1290             


Ile Ala  Glu Met Asn Gln Asn  Ile Ala Leu Tyr Asn  Asp Gly Lys 
    1295                 1300                 1305             


Met His  Gln Pro Ile Leu Lys  Val Arg Ile Ser Glu  Pro Lys Gly 
    1310                 1315                 1320             


Ala Lys  Tyr Gln Ile Gly Gln  Thr Gly Asn Lys Ala  Asp Lys Phe 
    1325                 1330                 1335             


Val Glu  Ala Gln Lys Gly Thr  Asn Leu Tyr Phe Ala  Ile Tyr Glu 
    1340                 1345                 1350             


Asp Glu  Asp Arg Asn Arg Thr  Tyr Asn Thr Ile Pro  Leu Asn Glu 
    1355                 1360                 1365             


Val Ala  Glu Arg Leu Lys Gln  Gly Leu Pro Pro Val  Pro Glu Lys 
    1370                 1375                 1380             


Asn Glu  Lys Glu Val Pro Leu  Lys Phe Tyr Leu Ser  Pro Asn Asp 
    1385                 1390                 1395             


Leu Val  Tyr Val Pro Thr Glu  Glu Glu Ile Leu Met  Lys Glu Cys 
    1400                 1405                 1410             


Lys Leu  Asn Lys Asn Arg Ile  Tyr Lys Met Val Ser  Ser Thr Gly 
    1415                 1420                 1425             


Asn Gln  Cys Phe Phe Ile Pro  Phe Arg Val Ala Val  Ser Ile Val 
    1430                 1435                 1440             


Asn Lys  Met Glu Tyr Ser Pro  Leu Asn Lys Met Glu  Lys Ala Ile 
    1445                 1450                 1455             


Ser Ser  Glu Met Ile Lys Ala  Val Cys Trp Lys Leu  Glu Val Asp 
    1460                 1465                 1470             


Arg Leu  Gly Asn Ile Ile Arg  Val Val Lys 
    1475                 1480             


<210>  1290
<211>  1485
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1290

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Ile Ser Ile Asp Asp Asn Gly Lys Tyr Ile Lys Asp Ser Ile 
            20                  25                  30          


Lys Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Ser Asn 
        35                  40                  45              


Phe Ser Lys Gly Val Thr Leu Ser Gln Thr Ser Glu Arg Thr Ser Phe 
    50                  55                  60                  


Arg Gly Ala Arg Arg Leu Arg Glu Arg Thr Leu Leu Arg Arg Glu Arg 
65                  70                  75                  80  


Leu His Arg Ile Leu His Leu Leu Gly Phe Leu Pro Lys His Tyr Glu 
                85                  90                  95      


Lys Ala Ile Gly Trp Asp Arg Asn Asp Asn Lys Thr Tyr Gly Lys Phe 
            100                 105                 110         


Ile Asn His Ala Glu Pro Lys Leu Ala Trp Cys Lys Asp Asn Asn Gly 
        115                 120                 125             


Lys Met Arg Phe Leu Phe Met Asp Ser Phe His Asp Met Leu Ser Glu 
    130                 135                 140                 


Phe Ser Glu Lys His Pro Glu Leu Leu Ala Asn Gly Lys Lys Ile Pro 
145                 150                 155                 160 


Leu Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Tyr Lys Ala 
                165                 170                 175     


Ile Thr Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys 
            180                 185                 190         


Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Asp Arg Asp Ser Glu Ala 
        195                 200                 205             


Asn Lys Lys Glu Tyr Leu Glu Leu Lys Val Ile Gly Val Glu Ala Thr 
    210                 215                 220                 


Gly Glu Lys Lys Gly Asn Asp Ile Trp Tyr Asn Ile His Leu Glu Asn 
225                 230                 235                 240 


Gly Trp Ile Tyr Arg Arg Ser Ser Lys Asn Pro Leu Asn Asp Trp Leu 
                245                 250                 255     


Gly Lys Val Lys Ser Phe Ile Val Thr Thr Asp Leu Asp Ala Asp Gly 
            260                 265                 270         


Asn Pro Lys Lys Asp Lys Asp Gly Asn Ile Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Pro Ser Glu Gly Asp Trp Thr Leu Ile Lys Lys Arg Thr Glu Ser Leu 
    290                 295                 300                 


Leu Glu Asn Ser Gly Gln Thr Val Gly Gln Phe Ile Tyr Asn His Ile 
305                 310                 315                 320 


Leu Glu Glu Pro Ser Ala Lys Ile Arg Gly Asn Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Asp Glu Leu Lys Ala Ile Leu Glu Lys Gln 
            340                 345                 350         


Lys Glu Phe His His Glu Leu Ser Ser Glu Ser Leu Leu Thr Glu Cys 
        355                 360                 365             


Ala Lys Glu Leu Tyr Lys Arg Asn Ile Ala His Gln Glu Val Leu Leu 
    370                 375                 380                 


Lys Lys Gly Met Ile His Leu Leu Leu Asn Asp Ile Leu Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ile Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu 
                405                 410                 415     


Asn Tyr Lys Tyr Val Asp Lys Lys Ser Gly Asp Ile Lys Val Lys Ser 
            420                 425                 430         


Ile Lys Cys Ile Ala Lys Ser Asn Pro Phe Phe Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Val Ser Asn Leu Arg Leu Tyr Arg Lys Ser Asp Asp Lys 
    450                 455                 460                 


Asp Val Thr Ser Glu His Leu Val Thr Thr Asp Asp Tyr Ala Asn Leu 
465                 470                 475                 480 


Phe Thr Tyr Leu Asn Asn Arg Lys Asp Ile Lys Gln Ser Val Leu Leu 
                485                 490                 495     


Lys Glu Tyr Phe Lys Ile Lys Lys Val Lys Thr Ile Asn Gly Ala Glu 
            500                 505                 510         


Tyr Pro Val Arg Trp Asn Tyr Ile Asp Asp Asp Asn Lys Thr Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg Tyr Asp Ile Leu His Ala Leu Ser Lys Thr Glu 
    530                 535                 540                 


Leu Lys Ser Glu Trp Leu Thr Pro Glu Arg Thr Tyr Arg Leu Trp His 
545                 550                 555                 560 


Leu Leu Tyr Ser Ile Glu Ser Lys Glu Glu Thr Lys Lys Ala Leu Asn 
                565                 570                 575     


Lys Leu Asn Leu Ser Asp Asp Phe Thr Glu Ala Phe Leu Thr Ile Lys 
            580                 585                 590         


Pro Phe Lys Lys Glu Tyr Gly Ser Tyr Ser Glu Lys Ala Ile Lys Lys 
        595                 600                 605             


Leu Leu Ser Leu Met Arg Met Gly Tyr Leu Trp Ser Glu Asp Glu Ala 
    610                 615                 620                 


Ile Lys Gln Val Gly Ala Glu Ile Glu Lys Thr Ile Glu Gly Glu Leu 
625                 630                 635                 640 


Pro Asp Tyr Val Leu Gln Arg Leu Glu Lys Ser Gln His Asn Tyr Asn 
                645                 650                 655     


Lys Ile Ser Asp Phe Ser Gly Leu Pro Leu Trp Leu Ala Ser Tyr Ile 
            660                 665                 670         


Ile Tyr Gly Arg His Ser Glu Ala Lys Glu Ile Ile His Trp Asp Asn 
        675                 680                 685             


Pro Glu Ser Met Met Ala Tyr Ile Lys Asp Phe Arg Gln His Ser Met 
    690                 695                 700                 


Arg Asn Pro Ile Val Glu Gln Cys Val Leu Glu Thr Leu Arg Thr Val 
705                 710                 715                 720 


His Asp Ile Trp Lys Glu Ile Gly His Ile Asp Glu Ile His Ile Glu 
                725                 730                 735     


Leu Gly Arg Glu Met Lys Ser Thr Ser Glu Gln Arg Lys Ala Ile Thr 
            740                 745                 750         


Gln Asn Val Leu His Asn Gln Asn Thr Asn Ile Arg Ile Lys Leu Leu 
        755                 760                 765             


Leu Gln Glu Leu Lys Asn Asp Asn Asn Ile Thr Gly Val Arg Pro Tyr 
    770                 775                 780                 


Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala Leu 
785                 790                 795                 800 


Gln Gly Leu Thr Lys Glu Asp Lys Asp Tyr Asn Asp Ile Leu Lys Ile 
                805                 810                 815     


Ser Gln Thr Ala Gln Pro Thr Ser Asn Glu Leu Thr Arg Tyr Lys Leu 
            820                 825                 830         


Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Ala Ile Ser 
        835                 840                 845             


Leu Ser Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Val Ile Pro 
    850                 855                 860                 


Gln Ser Arg Tyr Phe Asp Asp Ser Leu Ser Asn Lys Val Ile Cys Glu 
865                 870                 875                 880 


Ser Glu Val Asn Thr Leu Lys Lys Ala Met Leu Gly Met Glu Phe Ile 
                885                 890                 895     


Lys Thr Cys Gly Gly Gln Lys Val His Cys Thr Leu Leu Gly Asp Val 
            900                 905                 910         


Glu Ile Tyr Asn Glu Glu Asp Tyr Lys Ala Phe Val Asn Glu His Tyr 
        915                 920                 925             


Ser Asn Asn Arg Thr Lys Arg Asn Lys Leu Leu Met Glu Asp Ile Pro 
    930                 935                 940                 


Gln Glu Phe Ile Asn Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys 
945                 950                 955                 960 


Met Val Lys Gln Leu Leu Ser Ala Ile Val Arg Gly Gln Glu Glu Val 
                965                 970                 975     


Glu Ala Thr Ser Lys Phe Val Ile Pro Cys Thr Gly Gly Ile Thr Asp 
            980                 985                 990         


Arg Leu Lys Lys Asp Trp Gly Leu  Asn Asp Ile Trp Asn  Asn Leu Val 
        995                 1000                 1005             


Ser Pro  Arg Phe Gln Arg Met  Asn Ala Leu Thr Gly  Ser Glu Tyr 
    1010                 1015                 1020             


Phe Gly  His Trp Glu Asn Lys  Glu Gly Lys Arg Val  Phe Gln Thr 
    1025                 1030                 1035             


Thr Met  Pro Ile Gln Leu Gln  Gln Gly Phe Ser Lys  Lys Arg Ile 
    1040                 1045                 1050             


Asp His  Arg His His Ala Met  Asp Ala Leu Val Ile  Ala Cys Ala 
    1055                 1060                 1065             


Ser Arg  Asn Ile Ile Ser Tyr  Leu Asn Asn Glu Thr  Ala Gln Asp 
    1070                 1075                 1080             


Thr Glu  Arg Arg Arg Asp Leu  Arg Gln Lys Leu Cys  Asp Lys Gly 
    1085                 1090                 1095             


Gly Ser  Val Arg Lys Pro Trp  Asp Ser Phe Thr Gln  Asp Thr Gln 
    1100                 1105                 1110             


Tyr Ala  Leu Lys Asp Ile Ile  Val Thr Phe Lys Asn  Tyr Val Arg 
    1115                 1120                 1125             


Ile Ile  Asn Lys Ala Thr Asn  Tyr Tyr Glu His Tyr  Lys Thr Asp 
    1130                 1135                 1140             


Gly Lys  Lys Gly Met Ile Ser  Gln Lys Gly Asp Glu  Leu Trp Ala 
    1145                 1150                 1155             


Ile Arg  Lys Pro Met His Lys  Glu Thr Val Phe Gly  Lys Val Asn 
    1160                 1165                 1170             


Leu Arg  Arg Pro Lys Arg Val  Ser Leu Ser Lys Ala  Ile Asp Ser 
    1175                 1180                 1185             


Val Asp  Val Ile Cys Asn Arg  Lys Leu Lys Lys Tyr  Ile Lys Ala 
    1190                 1195                 1200             


Arg Leu  Asn Glu Gly Leu Gln  Lys Lys Gln Ile Leu  Asn Ile Phe 
    1205                 1210                 1215             


Lys Glu  Val Asn Asn Val Trp  Glu Gly Ile Asp Val  Asn Lys Ile 
    1220                 1225                 1230             


Asp Ile  Trp Glu Met Ser Asn  Asp Lys Glu Pro Met  Val Ala Thr 
    1235                 1240                 1245             


Arg Lys  Gln Leu Asp Thr Ser  Phe Asp Glu Lys Lys  Ile Arg Ser 
    1250                 1255                 1260             


Ile Ser  Asp Thr Gly Ile Gln  Lys Ile Leu Leu Asn  Tyr Leu Ile 
    1265                 1270                 1275             


Thr Lys  Asn Asn Asn Pro Glu  Leu Ala Phe Thr Pro  Glu Gly Ile 
    1280                 1285                 1290             


Gln Glu  Met Asn Ser Asn Ile  Ser Glu Tyr Asn Asp  His Lys Lys 
    1295                 1300                 1305             


His Gln  Pro Ile Tyr Lys Val  Arg Val Thr Glu Pro  Leu Gly Ala 
    1310                 1315                 1320             


Lys Tyr  Pro Ile Gly Thr Met  Gly Asn Asn Pro Lys  Lys Phe Val 
    1325                 1330                 1335             


Glu Ala  Gln Ala Gly Thr Asn  Leu Tyr Phe Ala Ile  Tyr Glu Asp 
    1340                 1345                 1350             


Lys Glu  Gly Asn Arg Asn Tyr  Asp Thr Ile Pro Leu  Asn Ile Ala 
    1355                 1360                 1365             


Ala Glu  Arg Leu Asn Gln Gln  Leu Ser Pro Val Pro  Glu Thr Asn 
    1370                 1375                 1380             


Glu Asn  Asn Thr Pro Leu Lys  Phe Tyr Leu Ser Pro  Asn Asp Leu 
    1385                 1390                 1395             


Val Tyr  Val Pro Ser Glu Glu  Glu Arg His Ser Gly  Asn Asn Ile 
    1400                 1405                 1410             


Gln Leu  Asp Lys Asn Arg Ile  Tyr Lys Met Val Ser  Ser Thr Ser 
    1415                 1420                 1425             


Lys Gln  Cys Phe Phe Ile His  His Arg Val Ala Ile  Ser Leu Val 
    1430                 1435                 1440             


Asp Lys  Lys Glu Phe Ser Ala  Gln Asn Lys Met Glu  Arg Ser Ile 
    1445                 1450                 1455             


Asp Asn  Glu Gln Val Met Ile  Lys Ser Val Cys Trp  Lys Leu Ile 
    1460                 1465                 1470             


Thr Asp  Arg Leu Gly Asn Ile  Ile Lys Val Ile Lys  
    1475                 1480                 1485 


<210>  1291
<211>  1485
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1291

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Ile Ser Ile Asp Asp Asn Gly Lys Tyr Ile Lys Asp Ser Ile 
            20                  25                  30          


Lys Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Ser Asn 
        35                  40                  45              


Phe Ser Lys Gly Val Thr Leu Ser Gln Thr Ser Glu Arg Thr Ser Phe 
    50                  55                  60                  


Arg Gly Ala Arg Arg Leu Arg Glu Arg Thr Leu Leu Arg Arg Glu Arg 
65                  70                  75                  80  


Leu His Arg Ile Leu His Leu Leu Gly Phe Leu Pro Lys His Tyr Glu 
                85                  90                  95      


Lys Ala Ile Gly Trp Asp Arg Asn Asp Asn Lys Thr Tyr Gly Lys Phe 
            100                 105                 110         


Ile Asn His Ala Glu Pro Lys Leu Ala Trp Cys Lys Asp Asn Asn Gly 
        115                 120                 125             


Lys Met Arg Phe Leu Phe Met Asp Ser Phe His Asp Met Leu Ser Glu 
    130                 135                 140                 


Phe Ser Glu Lys His Pro Glu Leu Leu Ala Asn Gly Lys Lys Ile Pro 
145                 150                 155                 160 


Leu Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Tyr Lys Ala 
                165                 170                 175     


Ile Thr Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys 
            180                 185                 190         


Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Asp Arg Asp Ser Glu Ala 
        195                 200                 205             


Asn Lys Lys Glu Tyr Leu Glu Leu Lys Val Ile Gly Val Glu Ala Thr 
    210                 215                 220                 


Gly Glu Lys Lys Gly Asn Asp Ile Trp Tyr Asn Ile His Leu Glu Asn 
225                 230                 235                 240 


Gly Trp Ile Tyr Arg Arg Ser Ser Lys Asn Pro Leu Asn Asp Trp Leu 
                245                 250                 255     


Gly Lys Val Lys Ser Phe Ile Val Thr Thr Asp Leu Asp Ala Asp Gly 
            260                 265                 270         


Asn Pro Lys Lys Asp Lys Asp Gly Asn Ile Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Pro Ser Glu Gly Asp Trp Thr Leu Ile Lys Lys Arg Thr Glu Ser Leu 
    290                 295                 300                 


Leu Glu Asn Ser Gly Gln Thr Val Gly Gln Phe Ile Tyr Asn His Ile 
305                 310                 315                 320 


Leu Glu Glu Pro Ser Ala Lys Ile Arg Gly Asn Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Asp Glu Leu Lys Ala Ile Leu Glu Lys Gln 
            340                 345                 350         


Lys Glu Phe His His Glu Leu Ser Ser Glu Ser Leu Leu Thr Glu Cys 
        355                 360                 365             


Ala Lys Glu Leu Tyr Lys Arg Asn Ile Ala His Gln Glu Val Leu Leu 
    370                 375                 380                 


Lys Lys Gly Ile Ile His Leu Leu Leu Asn Asp Ile Leu Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ile Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu 
                405                 410                 415     


Asn Tyr Lys Tyr Val Asp Lys Lys Ser Gly Asp Ile Lys Val Lys Ser 
            420                 425                 430         


Ile Lys Cys Ile Ala Lys Ser Asn Pro Phe Phe Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Val Ser Asn Leu Arg Leu Tyr Arg Lys Ser Asp Asp Lys 
    450                 455                 460                 


Asp Val Thr Ser Glu His Leu Val Thr Thr Asp Asp Tyr Ala Asn Leu 
465                 470                 475                 480 


Phe Thr Tyr Leu Asn Asn Arg Lys Asp Ile Lys Gln Ser Val Leu Leu 
                485                 490                 495     


Lys Glu Tyr Phe Lys Ile Lys Lys Val Lys Thr Ile Asn Gly Ala Glu 
            500                 505                 510         


Tyr Pro Val Arg Trp Asn Tyr Ile Asp Asp Asp Asn Lys Thr Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg Tyr Asp Ile Leu His Ala Leu Ser Lys Thr Glu 
    530                 535                 540                 


Leu Lys Ser Glu Trp Leu Thr Pro Glu Arg Thr Tyr Arg Leu Trp His 
545                 550                 555                 560 


Leu Leu Tyr Ser Ile Glu Ser Lys Glu Glu Thr Lys Lys Ala Leu Asn 
                565                 570                 575     


Lys Leu Asn Leu Ser Asp Asp Phe Thr Glu Ala Phe Leu Thr Ile Lys 
            580                 585                 590         


Pro Phe Lys Lys Glu Tyr Gly Ser Tyr Ser Glu Lys Ala Ile Lys Lys 
        595                 600                 605             


Leu Leu Ser Leu Met Arg Met Gly Tyr Leu Trp Ser Glu Asp Glu Ala 
    610                 615                 620                 


Ile Lys Gln Val Gly Ala Glu Ile Glu Lys Thr Ile Glu Gly Glu Leu 
625                 630                 635                 640 


Pro Asp Tyr Val Leu Gln Arg Leu Glu Lys Ser Gln His Asn Tyr Asn 
                645                 650                 655     


Lys Ile Ser Asp Phe Ser Gly Leu Pro Leu Trp Leu Ala Ser Tyr Ile 
            660                 665                 670         


Ile Tyr Gly Arg His Ser Glu Ala Lys Glu Ile Ile His Trp Asp Asn 
        675                 680                 685             


Pro Glu Ser Met Met Ala Tyr Ile Lys Asp Phe Arg Gln His Ser Met 
    690                 695                 700                 


Arg Asn Pro Ile Val Glu Gln Cys Val Leu Glu Thr Leu Arg Thr Val 
705                 710                 715                 720 


His Asp Ile Trp Lys Glu Ile Gly His Ile Asp Glu Ile His Ile Glu 
                725                 730                 735     


Leu Gly Arg Glu Met Lys Ser Thr Ser Glu Gln Arg Lys Ala Ile Thr 
            740                 745                 750         


Gln Asn Val Leu His Asn Gln Asn Thr Asn Ile Arg Ile Lys Leu Leu 
        755                 760                 765             


Leu Gln Glu Leu Lys Asn Asp Asn Asn Ile Thr Gly Val Arg Pro Tyr 
    770                 775                 780                 


Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala Leu 
785                 790                 795                 800 


Gln Gly Leu Thr Lys Glu Asp Lys Asp Tyr Asn Asp Ile Leu Lys Ile 
                805                 810                 815     


Ser Gln Thr Ala Gln Pro Thr Ser Asn Glu Leu Thr Arg Tyr Lys Leu 
            820                 825                 830         


Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Ala Ile Ser 
        835                 840                 845             


Leu Ser Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Val Ile Pro 
    850                 855                 860                 


Gln Ser Arg Tyr Phe Asp Asp Ser Leu Ser Asn Lys Val Ile Cys Glu 
865                 870                 875                 880 


Ser Glu Val Asn Thr Leu Lys Lys Ala Met Leu Gly Met Glu Phe Ile 
                885                 890                 895     


Lys Thr Cys Gly Gly Gln Lys Val His Cys Thr Leu Leu Gly Asp Val 
            900                 905                 910         


Glu Ile Tyr Asn Glu Glu Asp Tyr Lys Ala Phe Val Asn Glu His Tyr 
        915                 920                 925             


Ser Asn Asn Arg Thr Lys Arg Asn Lys Leu Leu Met Glu Asp Ile Pro 
    930                 935                 940                 


Gln Glu Phe Ile Asn Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys 
945                 950                 955                 960 


Met Val Lys Gln Leu Leu Ser Ala Ile Val Arg Gly Gln Glu Glu Val 
                965                 970                 975     


Glu Ala Thr Ser Lys Phe Val Ile Pro Cys Thr Gly Gly Ile Thr Asp 
            980                 985                 990         


Arg Leu Lys Lys Asp Trp Gly Leu  Asn Asp Ile Trp Asn  Asn Leu Val 
        995                 1000                 1005             


Ser Pro  Arg Phe Gln Arg Met  Asn Ala Leu Thr Gly  Ser Glu Tyr 
    1010                 1015                 1020             


Phe Gly  His Trp Glu Asn Lys  Glu Gly Lys Arg Val  Phe Gln Thr 
    1025                 1030                 1035             


Thr Met  Pro Ile Gln Leu Gln  Gln Gly Phe Ser Lys  Lys Arg Ile 
    1040                 1045                 1050             


Asp His  Arg His His Ala Met  Asp Ala Leu Val Ile  Ala Cys Ala 
    1055                 1060                 1065             


Ser Arg  Asn Ile Ile Ser Tyr  Leu Asn Asn Glu Thr  Ala Gln Asp 
    1070                 1075                 1080             


Thr Glu  Arg Arg Arg Asp Leu  Arg Gln Lys Leu Cys  Asp Lys Gly 
    1085                 1090                 1095             


Gly Ser  Val Arg Lys Pro Trp  Asp Ser Phe Thr Gln  Asp Thr Gln 
    1100                 1105                 1110             


Tyr Ala  Leu Lys Asp Ile Ile  Val Thr Phe Lys Asn  Tyr Val Arg 
    1115                 1120                 1125             


Ile Ile  Asn Lys Ala Thr Asn  Tyr Tyr Glu His Tyr  Lys Thr Asp 
    1130                 1135                 1140             


Gly Lys  Lys Gly Met Ile Ser  Gln Lys Gly Asp Glu  Leu Trp Ala 
    1145                 1150                 1155             


Ile Arg  Lys Pro Met His Lys  Glu Thr Val Phe Gly  Lys Val Asn 
    1160                 1165                 1170             


Leu Arg  Arg Pro Lys Arg Val  Ser Leu Ser Lys Ala  Ile Asp Ser 
    1175                 1180                 1185             


Val Asp  Ile Ile Cys Asn Arg  Lys Leu Lys Lys Tyr  Ile Lys Ala 
    1190                 1195                 1200             


Arg Leu  Asn Glu Gly Leu Gln  Lys Lys Gln Ile Leu  Asn Ile Phe 
    1205                 1210                 1215             


Lys Glu  Leu Asn Asn Val Trp  Glu Gly Ile Asp Val  Asn Lys Ile 
    1220                 1225                 1230             


Asp Ile  Trp Glu Met Ser Asn  Asp Lys Glu Pro Met  Val Ala Thr 
    1235                 1240                 1245             


Arg Lys  Gln Leu Asp Thr Ser  Phe Asp Glu Lys Lys  Ile Arg Ser 
    1250                 1255                 1260             


Ile Ser  Asp Thr Gly Ile Gln  Lys Ile Leu Leu Asn  Tyr Leu Ile 
    1265                 1270                 1275             


Thr Lys  Asn Asn Asn Pro Glu  Leu Ala Phe Thr Pro  Glu Gly Ile 
    1280                 1285                 1290             


Gln Glu  Met Asn Ser Asn Ile  Ser Glu Tyr Asn Asp  His Lys Lys 
    1295                 1300                 1305             


His Gln  Pro Ile Tyr Lys Val  Arg Val Thr Glu Pro  Leu Gly Ala 
    1310                 1315                 1320             


Lys Tyr  Pro Ile Gly Thr Met  Gly Asn Asn Pro Lys  Lys Phe Val 
    1325                 1330                 1335             


Glu Ala  Gln Ala Gly Thr Asn  Leu Tyr Phe Ala Ile  Tyr Glu Asp 
    1340                 1345                 1350             


Lys Glu  Gly Lys Arg Asn Tyr  Asp Thr Ile Pro Leu  Asn Ile Ala 
    1355                 1360                 1365             


Ala Glu  Arg Leu Asn Gln Gln  Leu Ser Pro Val Pro  Glu Thr Asn 
    1370                 1375                 1380             


Glu Asn  Asn Thr Pro Leu Lys  Phe Tyr Leu Ser Pro  Asn Asp Leu 
    1385                 1390                 1395             


Val Tyr  Val Pro Ser Glu Glu  Glu Arg His Ser Gly  Asn Asn Ile 
    1400                 1405                 1410             


Gln Leu  Asp Lys Asn Arg Ile  Tyr Lys Met Val Ser  Ser Thr Ser 
    1415                 1420                 1425             


Lys Arg  Cys Phe Phe Ile His  His Arg Val Ala Ile  Ser Leu Val 
    1430                 1435                 1440             


Asp Lys  Lys Glu Phe Ser Ala  Gln Asn Lys Met Glu  Arg Ser Ile 
    1445                 1450                 1455             


Asp Asn  Glu Gln Val Met Ile  Lys Ser Val Cys Trp  Lys Leu Ile 
    1460                 1465                 1470             


Thr Asp  Arg Leu Gly Asn Ile  Ile Lys Val Ile Lys  
    1475                 1480                 1485 


<210>  1292
<211>  1485
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1292

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Ile Ser Ile Asp Asp Asn Gly Lys Tyr Ile Lys Asp Ser Ile 
            20                  25                  30          


Lys Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Ser Asn 
        35                  40                  45              


Phe Ser Lys Gly Val Thr Leu Ser Gln Thr Ser Glu Arg Thr Ser Phe 
    50                  55                  60                  


Arg Gly Ala Arg Arg Leu Arg Glu Arg Thr Leu Leu Arg Arg Glu Arg 
65                  70                  75                  80  


Leu His Arg Ile Leu His Leu Leu Gly Phe Leu Pro Lys His Tyr Glu 
                85                  90                  95      


Lys Ala Ile Gly Trp Asp Arg Asn Asp Asn Lys Thr Tyr Gly Lys Phe 
            100                 105                 110         


Ile Asn His Ala Glu Pro Lys Leu Ala Trp Cys Lys Asp Asn Asn Gly 
        115                 120                 125             


Lys Met Arg Phe Leu Phe Met Asp Ser Phe His Asp Met Leu Ser Glu 
    130                 135                 140                 


Phe Ser Glu Lys His Pro Glu Leu Leu Ala Asn Gly Lys Lys Ile Pro 
145                 150                 155                 160 


Leu Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Tyr Lys Ala 
                165                 170                 175     


Ile Thr Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys 
            180                 185                 190         


Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Asp Arg Asp Ser Glu Ala 
        195                 200                 205             


Asn Lys Lys Glu Tyr Leu Glu Leu Lys Val Ile Gly Val Glu Ala Thr 
    210                 215                 220                 


Gly Glu Lys Lys Gly Asn Asp Ile Trp Tyr Asn Ile His Leu Glu Asn 
225                 230                 235                 240 


Gly Trp Ile Tyr Arg Arg Ser Ser Lys Asn Pro Leu Asn Asp Trp Leu 
                245                 250                 255     


Gly Lys Val Lys Ser Phe Ile Val Thr Thr Asp Leu Asp Ala Asp Gly 
            260                 265                 270         


Asn Pro Lys Lys Asp Lys Asp Gly Asn Ile Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Pro Ser Glu Gly Asp Trp Thr Leu Ile Lys Lys Arg Thr Glu Ser Leu 
    290                 295                 300                 


Leu Glu Asn Ser Gly Gln Thr Val Gly Gln Phe Ile Tyr Asn His Ile 
305                 310                 315                 320 


Leu Glu Glu Pro Ser Ala Lys Ile Arg Gly Asn Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Asp Glu Leu Lys Ala Ile Leu Glu Lys Gln 
            340                 345                 350         


Lys Glu Phe His His Glu Leu Ser Ser Glu Ser Leu Leu Thr Glu Cys 
        355                 360                 365             


Ala Lys Glu Leu Tyr Lys Arg Asn Ile Ala His Gln Glu Val Leu Leu 
    370                 375                 380                 


Lys Lys Gly Met Ile His Leu Leu Leu Asn Asp Ile Leu Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ile Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu 
                405                 410                 415     


Asn Tyr Lys Tyr Val Asp Lys Lys Ser Gly Asp Ile Lys Val Lys Ser 
            420                 425                 430         


Ile Lys Cys Ile Ala Lys Ser Asn Pro Phe Phe Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Val Ser Asn Leu Arg Leu Tyr Arg Lys Ser Asp Asp Lys 
    450                 455                 460                 


Asp Val Thr Ser Glu His Leu Val Thr Thr Asp Asp Tyr Ala Asn Leu 
465                 470                 475                 480 


Phe Thr Tyr Leu Asn Asn Arg Lys Asp Ile Lys Gln Ser Val Leu Leu 
                485                 490                 495     


Lys Glu Tyr Phe Lys Ile Lys Lys Val Lys Thr Ile Asn Gly Ala Glu 
            500                 505                 510         


Tyr Pro Val Arg Trp Asn Tyr Ile Asp Asp Asp Asn Lys Thr Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg Tyr Asp Ile Leu His Ala Leu Ser Lys Thr Glu 
    530                 535                 540                 


Leu Lys Ser Glu Trp Leu Thr Pro Glu Arg Thr Tyr Arg Leu Trp His 
545                 550                 555                 560 


Leu Leu Tyr Ser Ile Glu Ser Lys Glu Glu Thr Lys Lys Ala Leu Asn 
                565                 570                 575     


Lys Leu Asn Leu Ser Asp Asp Phe Thr Glu Ala Phe Leu Thr Ile Lys 
            580                 585                 590         


Pro Phe Lys Lys Glu Tyr Gly Ser Tyr Ser Glu Lys Ala Ile Lys Lys 
        595                 600                 605             


Leu Leu Ser Leu Met Arg Met Gly Tyr Leu Trp Ser Glu Asp Glu Ala 
    610                 615                 620                 


Ile Lys Gln Val Gly Ala Glu Ile Glu Lys Thr Ile Glu Gly Glu Leu 
625                 630                 635                 640 


Pro Asp Tyr Val Leu Gln Arg Leu Glu Lys Ser Gln His Asn Tyr Asn 
                645                 650                 655     


Lys Ile Ser Asp Phe Ser Gly Leu Pro Leu Trp Leu Ala Ser Tyr Ile 
            660                 665                 670         


Ile Tyr Gly Arg His Ser Glu Ala Lys Glu Ile Ile His Trp Asp Asn 
        675                 680                 685             


Pro Glu Ser Met Met Ala Tyr Ile Lys Asp Phe Arg Gln His Ser Met 
    690                 695                 700                 


Arg Asn Pro Ile Val Glu Gln Cys Val Leu Glu Thr Leu Arg Thr Val 
705                 710                 715                 720 


His Asp Ile Trp Lys Glu Ile Gly His Ile Asp Glu Ile His Ile Glu 
                725                 730                 735     


Leu Gly Arg Glu Met Lys Ser Thr Ser Glu Gln Arg Lys Ala Ile Thr 
            740                 745                 750         


Gln Asn Val Leu His Asn Gln Asn Thr Asn Ile Arg Ile Lys Leu Leu 
        755                 760                 765             


Leu Gln Glu Leu Lys Asn Asp Asn Asn Ile Thr Gly Val Arg Pro Tyr 
    770                 775                 780                 


Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala Leu 
785                 790                 795                 800 


Gln Gly Leu Thr Lys Glu Asp Lys Asp Tyr Asn Asp Ile Leu Lys Ile 
                805                 810                 815     


Ser Gln Thr Ala Gln Pro Thr Ser Asn Glu Leu Thr Arg Tyr Lys Leu 
            820                 825                 830         


Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Ala Ile Ser 
        835                 840                 845             


Leu Ser Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Val Ile Pro 
    850                 855                 860                 


Gln Ser Arg Tyr Phe Asp Asp Ser Leu Ser Asn Lys Val Ile Cys Glu 
865                 870                 875                 880 


Ser Glu Val Asn Thr Leu Lys Lys Ala Met Leu Gly Met Glu Phe Ile 
                885                 890                 895     


Lys Thr Cys Gly Gly Gln Lys Val His Cys Thr Leu Leu Gly Asp Val 
            900                 905                 910         


Glu Ile Tyr Asn Glu Glu Asp Tyr Lys Ala Phe Val Asn Glu His Tyr 
        915                 920                 925             


Ser Asn Asn Arg Thr Lys Arg Asn Lys Leu Leu Met Glu Asp Ile Pro 
    930                 935                 940                 


Gln Glu Phe Ile Asn Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys 
945                 950                 955                 960 


Met Val Lys Gln Leu Leu Ser Ala Ile Val Arg Gly Gln Glu Glu Val 
                965                 970                 975     


Glu Ala Thr Ser Lys Phe Val Ile Pro Cys Thr Gly Gly Ile Thr Asp 
            980                 985                 990         


Arg Leu Lys Lys Asp Trp Gly Leu  Asn Asp Ile Trp Asn  Asn Leu Val 
        995                 1000                 1005             


Ser Pro  Arg Phe Gln Arg Met  Asn Ala Leu Thr Gly  Ser Glu Tyr 
    1010                 1015                 1020             


Phe Gly  His Trp Glu Asn Lys  Glu Gly Lys Arg Val  Phe Gln Thr 
    1025                 1030                 1035             


Thr Met  Pro Ile Gln Leu Gln  Gln Gly Phe Ser Lys  Lys Arg Ile 
    1040                 1045                 1050             


Asp His  Arg His His Ala Met  Asp Ala Leu Val Ile  Ala Cys Ala 
    1055                 1060                 1065             


Ser Arg  Asn Ile Ile Ser Tyr  Leu Asn Asn Glu Thr  Ala Gln Asp 
    1070                 1075                 1080             


Thr Glu  Arg Arg Arg Asp Leu  Arg Gln Lys Leu Cys  Asp Lys Gly 
    1085                 1090                 1095             


Gly Ser  Val Arg Lys Pro Trp  Asp Ser Phe Thr Gln  Asp Thr Gln 
    1100                 1105                 1110             


Tyr Ala  Leu Lys Asp Ile Ile  Val Thr Phe Lys Asn  Tyr Val Arg 
    1115                 1120                 1125             


Ile Ile  Asn Lys Ala Thr Asn  Tyr Tyr Glu His Tyr  Lys Thr Asp 
    1130                 1135                 1140             


Gly Lys  Lys Gly Met Ile Ser  Gln Lys Gly Asp Glu  Leu Trp Ala 
    1145                 1150                 1155             


Ile Arg  Lys Pro Met His Lys  Glu Thr Val Phe Gly  Lys Val Asn 
    1160                 1165                 1170             


Leu Arg  Arg Pro Lys Arg Val  Ser Leu Ser Lys Ala  Ile Asp Ser 
    1175                 1180                 1185             


Val Asp  Val Ile Cys Asn Arg  Lys Leu Lys Lys Tyr  Ile Lys Ala 
    1190                 1195                 1200             


Arg Leu  Asn Glu Gly Leu Gln  Lys Lys Gln Ile Leu  Asn Ile Phe 
    1205                 1210                 1215             


Lys Glu  Leu Asn Asn Val Trp  Glu Gly Ile Asp Val  Asn Lys Ile 
    1220                 1225                 1230             


Asp Ile  Trp Glu Met Ser Asn  Asp Lys Glu Pro Met  Val Ala Thr 
    1235                 1240                 1245             


Arg Lys  Gln Leu Asp Thr Ser  Phe Asp Glu Lys Lys  Ile Arg Ser 
    1250                 1255                 1260             


Ile Ser  Asp Thr Gly Ile Gln  Lys Ile Leu Leu Asn  Tyr Leu Ile 
    1265                 1270                 1275             


Thr Lys  Asn Asn Asn Pro Glu  Leu Ala Phe Thr Pro  Glu Gly Ile 
    1280                 1285                 1290             


Gln Glu  Met Asn Ser Asn Ile  Ser Glu Tyr Asn Asp  His Lys Glu 
    1295                 1300                 1305             


His Gln  Pro Ile Tyr Lys Val  Arg Val Thr Glu Pro  Leu Gly Ala 
    1310                 1315                 1320             


Lys Tyr  Pro Ile Gly Thr Met  Gly Asn Lys Pro Lys  Lys Phe Val 
    1325                 1330                 1335             


Glu Ala  Gln Ala Gly Thr Asn  Leu Tyr Phe Ala Ile  Tyr Glu Asp 
    1340                 1345                 1350             


Lys Glu  Gly Asn Arg Asn Tyr  Asp Thr Ile Pro Leu  Asn Ile Ala 
    1355                 1360                 1365             


Ala Glu  Arg Leu Asn Gln Gln  Leu Ser Pro Val Pro  Glu Thr Asn 
    1370                 1375                 1380             


Glu Asn  Asn Thr Pro Leu Lys  Phe Tyr Leu Ser Pro  Asn Asp Leu 
    1385                 1390                 1395             


Val Tyr  Val Pro Ser Glu Glu  Glu Arg His Ser Gly  Asn Asn Ile 
    1400                 1405                 1410             


Gln Leu  Asp Lys Asn Arg Ile  Tyr Lys Met Val Ser  Ser Thr Ser 
    1415                 1420                 1425             


Lys Gln  Cys Phe Phe Ile His  His Arg Val Ala Ile  Ser Leu Val 
    1430                 1435                 1440             


Asp Lys  Lys Glu Phe Ser Ala  Gln Asn Lys Met Glu  Arg Ser Ile 
    1445                 1450                 1455             


Asp Asn  Glu Gln Val Met Ile  Lys Ser Val Cys Trp  Lys Leu Ile 
    1460                 1465                 1470             


Thr Asp  Arg Leu Gly Asn Ile  Ile Lys Val Ile Lys  
    1475                 1480                 1485 


<210>  1293
<211>  1486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1293

Met Thr Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Ile Arg Glu Asp Glu Asn Glu Gly Val Asn Gln Ile Ile Asp Lys 
            20                  25                  30          


Gly Val Arg Ile Phe Ser Glu Gly Val Lys Ser Glu Asn Gly Lys Glu 
        35                  40                  45              


Ile Ser Arg Ala Ala Glu Arg Thr Ala Tyr Arg Ser Ala Arg Lys Ile 
    50                  55                  60                  


Lys Tyr Arg Arg Lys Leu Arg Lys Tyr Glu Thr Leu Lys Val Leu Ser 
65                  70                  75                  80  


Val Asn Gly Met Cys Pro Leu Ser Val Glu Glu Val Glu Gln Trp Lys 
                85                  90                  95      


Lys Ser Gly Phe Lys Glu Tyr Pro Leu Asn Pro Glu Phe Leu Asn Trp 
            100                 105                 110         


Leu Arg Thr Asn Glu Asp Lys Asn Ile Asn Pro Tyr Phe Phe Arg Asp 
        115                 120                 125             


Lys Ala Ser Lys Gln Lys Val Thr Leu Phe Glu Leu Gly Arg Ala Leu 
    130                 135                 140                 


Tyr His Ile Ala Gln Arg Arg Gly Phe Leu Ser Asn Arg Leu Asp Gln 
145                 150                 155                 160 


Ser Ala Glu Gly Val Phe Glu Glu His Asn Pro Gln Ile Gln Asn Leu 
                165                 170                 175     


Ile Glu Asp Leu Asp Ser Ser Asn Thr Ile Leu Asn Glu Leu Lys Glu 
            180                 185                 190         


Tyr Tyr Ile Asn Leu Gly Ile Ile Asp Glu Ser Glu Lys Ser Gly Phe 
        195                 200                 205             


Lys Lys Asp Leu Asp Glu Gly Glu Lys Asn Leu Lys Lys Leu Tyr Thr 
    210                 215                 220                 


Ser Leu Val Ser Ile Thr Lys Lys Asn Ala Asn Asp Ile Glu Thr Cys 
225                 230                 235                 240 


Lys Gln Glu Leu Ile Ala Arg Leu Asn Lys Lys Glu Asp Leu Gly Lys 
                245                 250                 255     


Val Lys Gly Glu Ile Lys Asp Ile Ser Gln Ala Met Leu Asp Gly Asn 
            260                 265                 270         


Phe Lys Thr Leu Gly Gln Tyr Phe Phe Ser Leu Tyr Asn Lys Thr Arg 
        275                 280                 285             


Ile Arg Asn Gln Tyr Thr Ser Arg Glu Glu His Tyr Leu Glu Glu Phe 
    290                 295                 300                 


Ile Ile Ile Cys Gln Thr Gln Gly Ile Glu Glu Ile Asn Thr Asn Glu 
305                 310                 315                 320 


Lys Leu Pro Glu Lys Lys Phe Thr Gly Leu Ala Lys Asp Leu Tyr Arg 
                325                 330                 335     


Ala Ile Phe Phe Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Ile Gly 
            340                 345                 350         


Lys Cys Ser Phe Glu Lys Asn Lys Ser Arg Cys Ala Val Ser His Pro 
        355                 360                 365             


Asp Tyr Glu Glu Phe Arg Met Trp Ser Tyr Leu Asn Thr Ile Lys Ile 
    370                 375                 380                 


Gly Thr Gln Ser Glu Lys Thr Leu Arg Phe Leu Thr Leu Glu Glu Lys 
385                 390                 395                 400 


Leu Lys Leu Val Pro Lys Phe Tyr Arg Lys Asn Asp Phe Asn Phe Glu 
                405                 410                 415     


Val Leu Ala Lys Glu Leu Val Glu Lys Gly Ala Ser Phe Gly Tyr Tyr 
            420                 425                 430         


Lys Ser Ser Lys Lys Asn Glu Phe Phe Tyr Trp Phe Asn Tyr Lys Pro 
        435                 440                 445             


Thr Asp Ser Val Ser Ala Cys Val Val Ser Ala Ser Leu Glu Asn Ala 
    450                 455                 460                 


Ile Gly Lys Asp Trp Lys Thr Lys Thr Phe Glu Tyr Gln Thr Ser Asn 
465                 470                 475                 480 


Thr Lys Lys Glu Glu Val Thr Lys Ser Val Asn Tyr Lys Asp Leu Trp 
                485                 490                 495     


His Leu Leu Ser Val Ala Thr Ser Asp Thr Tyr Leu Tyr Asp Phe Ala 
            500                 505                 510         


Ile Glu Lys Leu Lys Leu Glu Pro Lys Asn Ala Lys Ala Phe Ser Lys 
        515                 520                 525             


Thr Lys Leu Lys Lys Asp Phe Ala Ser Leu Ser Leu Ala Ala Ile Ala 
    530                 535                 540                 


Lys Ile Leu Pro Tyr Leu Lys Gln Gly Leu Leu Tyr Ser His Ala Val 
545                 550                 555                 560 


Phe Ile Ala Asn Ile Glu Asn Ile Val Asp Ala Asp Ile Trp Lys Asp 
                565                 570                 575     


Lys Glu Gln Gln Lys Phe Ile Gln Ser Lys Ile Val Asp Leu Ile Asp 
            580                 585                 590         


Asn Tyr Leu Val Glu Lys Ser Lys Leu Glu Ile Ile Asn Cys Leu Leu 
        595                 600                 605             


Lys Ile Tyr Asn Thr Glu Asp Ile Glu Gly Arg Lys Val Tyr Tyr Ser 
    610                 615                 620                 


Lys Glu Ala Glu Ser Leu Phe Glu Ala Asp Leu Arg Lys Lys Leu Val 
625                 630                 635                 640 


Pro Phe Tyr Lys Ala Asn Thr Ile Ser Glu Gln Gln Glu Gln Glu Ile 
                645                 650                 655     


Ile Phe Lys Asp Leu Phe Pro Val Leu Ile Glu Gln Leu Lys Lys Gln 
            660                 665                 670         


Glu Phe Ile Lys Ile Val Arg Leu Asp Lys Lys Ile Glu Ala Phe Leu 
        675                 680                 685             


Lys Gly Glu Asn Glu Glu Gly Gln Ile Phe Cys Asn Tyr Pro Glu Lys 
    690                 695                 700                 


Phe Lys Lys Leu Tyr His Pro Ser Asp Ile Glu Val Phe Lys Lys Lys 
705                 710                 715                 720 


Ile Ile Lys Asp Glu Trp Gly Asn Glu Lys Val Val Leu Gly Ser Pro 
                725                 730                 735     


Leu Thr Thr Ser Ile Lys Asn Pro Met Ala Met Arg Ala Leu His Gln 
            740                 745                 750         


Leu Arg Lys Val Leu Asn Thr Leu Ile Leu Asp Gly Gln Ile Asp Glu 
        755                 760                 765             


Lys Thr Arg Ile His Ile Glu Met Ala Arg Glu Leu Asn Asp Ala Asn 
    770                 775                 780                 


Lys Arg Lys Gly Ile Gln Asp Phe Gln Lys Glu Arg Glu Glu Leu Tyr 
785                 790                 795                 800 


Lys Ile Tyr Glu Ala Lys Ile Lys Glu Leu Tyr Gln Val Lys Thr Gly 
                805                 810                 815     


Lys Glu Leu Leu His Val Thr Asp Glu Asp Leu Gln Lys Phe Glu Trp 
            820                 825                 830         


Ala Leu Glu Gln Thr Lys Asp Gly Arg Ile Ile Thr Lys Glu Glu Leu 
        835                 840                 845             


Ile Lys Tyr Lys Ile Trp Glu Glu Gln Asn His Ile Cys Ile Tyr Thr 
    850                 855                 860                 


Gly Lys Thr Ile Ser Ile Asp Ser Phe Leu Gly Gly Asn Pro Lys Phe 
865                 870                 875                 880 


Asp Ile Glu His Thr Ile Pro Arg Ser Ile Ser Gln Asp Asn Ser Leu 
                885                 890                 895     


Met Asn Lys Thr Leu Cys Asp Lys Asp Phe Asn Arg Ser Val Lys Gly 
            900                 905                 910         


Lys Lys Met Pro Val Glu Leu Ser Asn Tyr Asn Glu Ile Leu Pro Arg 
        915                 920                 925             


Ile Ala His Trp Lys Lys Gln Ala Glu Glu Leu Thr Arg Gln Ile Glu 
    930                 935                 940                 


Thr Ile Ser Arg Ser Ile Lys Ser Ala Ala Thr Lys Val Ala Lys Asp 
945                 950                 955                 960 


Lys Asn Ile Arg Lys Arg His Tyr Leu Thr Leu Lys Arg Asp Tyr Ile 
                965                 970                 975     


Gln Gly Lys Tyr Asp Arg Phe Thr Trp Gly Glu Pro Lys Val Gly Phe 
            980                 985                 990         


Lys Asn Ser Gln Ile Pro Asp Thr  Gly Ile Ile Thr Lys  Tyr Ala Gln 
        995                 1000                 1005             


Ala Tyr  Leu Lys Ser Tyr Phe  Lys Arg Val Glu Ser  Val Lys Gly 
    1010                 1015                 1020             


Gly Ala  Val Ala Glu Phe Arg  Lys Leu Trp Gly Ile  Gln Glu Ser 
    1025                 1030                 1035             


Phe Ile  Asp Glu Asn Gly Phe  Lys His Phe Lys Glu  Lys Asp Arg 
    1040                 1045                 1050             


Ser Lys  His Thr His His Thr  Ile Asp Ala Ile Thr  Ile Ala Cys 
    1055                 1060                 1065             


Met Pro  Lys Asp Lys Tyr Asp  Leu Leu Ala His Ala  Trp Arg Leu 
    1070                 1075                 1080             


Glu Asp  Glu Gln Asn Lys Lys  Ala Ala Lys Ala Leu  Ile Glu Gln 
    1085                 1090                 1095             


Ala Lys  Pro Trp Lys Thr Phe  Lys Glu Asp Ile Glu  Lys Ile Glu 
    1100                 1105                 1110             


Ala Glu  Ile Leu Val Ser His  Phe Thr Pro Asp Asn  Val Lys Lys 
    1115                 1120                 1125             


Gln Ala  Lys Lys Ile Leu Arg  Val Arg Gly Lys Lys  Gln Tyr Val 
    1130                 1135                 1140             


Ala Glu  Ile Glu Arg Asn Ala  Asn Gly Lys Ala Ile  Pro Lys Lys 
    1145                 1150                 1155             


Asp Ala  Ser Gly Lys Ile Ile  Tyr Lys Leu Asn Glu  Arg Gly Asn 
    1160                 1165                 1170             


Lys Ile  Pro Ile Leu Gln Gln  Gly Asp Thr Ile Arg  Gly Ser Leu 
    1175                 1180                 1185             


His Gln  Asp Ser Ile Tyr Gly  Ala Ile Lys Asn Pro  Leu Asn Thr 
    1190                 1195                 1200             


Asn Glu  Ile Arg Tyr Val Ile  Arg Lys Asp Leu Glu  Ser Leu Lys 
    1205                 1210                 1215             


Ala Asn  Asp Ile Glu Asn Ile  Val Asp Glu Asn Val  Lys Ala Lys 
    1220                 1225                 1230             


Val Lys  Glu Ala Val Ala Asn  Lys Ile Leu Leu Ile  Ser Ser Asn 
    1235                 1240                 1245             


Pro Gln  Gln Lys Asn Lys Phe  Ala Glu Gly Lys Asn  Val Trp Met 
    1250                 1255                 1260             


Asn Glu  Glu Lys Lys Ile Pro  Ile Asn Lys Val Arg  Ile Tyr Ala 
    1265                 1270                 1275             


Asn Ser  Val Lys Asn Pro Ile  Glu Ile Lys Lys His  Ser Thr Leu 
    1280                 1285                 1290             


Ser Lys  Ser Lys His Glu His  Lys Gln Thr Val Tyr  Gly Gln Asn 
    1295                 1300                 1305             


Asp Glu  Asn Tyr Ala Met Ala  Leu Tyr Glu Leu Asp  Gly Lys Arg 
    1310                 1315                 1320             


Glu Phe  Glu Leu Ile Asn Asn  Phe Asn Leu Ala Lys  Leu Leu Lys 
    1325                 1330                 1335             


Gln Gly  Gln Gly Tyr Tyr Pro  Leu His Lys Glu Lys  Glu Ile Lys 
    1340                 1345                 1350             


Gly Lys  Lys Val Leu Ile Pro  Ile Glu Lys Arg Asn  Asn Arg Asp 
    1355                 1360                 1365             


Val Val  Leu Lys Arg Gly Gln  Gln Val Val Phe Tyr  Asp Lys Thr 
    1370                 1375                 1380             


Val Glu  Asn Ser Lys Asp Ile  Ser Glu Ile Val Asp  Phe Arg Gly 
    1385                 1390                 1395             


Arg Val  Tyr Leu Ile Glu Gly  Leu Thr Ile Gln Arg  Gln Lys Asp 
    1400                 1405                 1410             


Lys Lys  Thr Gly Lys Val Asn  Glu Tyr Gly Ile Ile  Gln Leu Arg 
    1415                 1420                 1425             


Tyr Phe  Lys Glu Ala Arg Lys  Ser Glu Glu Ile Ser  Lys Asp Ser 
    1430                 1435                 1440             


Phe Lys  Pro Asp Gly Glu Phe  Lys Ile Asn Glu Asn  Lys Pro Thr 
    1445                 1450                 1455             


Arg Lys  Met Asn His Asn Gln  Phe Thr Ala Phe Val  Glu Gly Ile 
    1460                 1465                 1470             


Asp Phe  Lys Val Leu Pro Ser  Gly Lys Phe Gln Lys  Ile 
    1475                 1480                 1485     


<210>  1294
<211>  1487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1294

Met Lys Asn Val Leu Gly Leu Asp Leu Gly Ser Asn Ser Val Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Thr Glu Asp Asn Gly Asn Pro Ile Gly Ser Ile Lys 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Ser Phe 
        35                  40                  45              


Glu Lys Gly Asp Thr Val Ser Gln Thr Ala Gln Arg Thr Leu Tyr Arg 
    50                  55                  60                  


Gly Lys Arg Arg Leu Ile Glu Arg His Val Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


Cys Arg Val Leu His Ile Met Asn Phe Leu Pro Asp His Phe Asp Lys 
                85                  90                  95      


Leu Leu Gly Trp Asp Lys Thr Asp Asn Lys Thr Tyr Gly Lys Phe Ile 
            100                 105                 110         


Asp Asp Ser Glu Pro Lys Leu Ala Trp Arg Gln Asn Lys Asn Glu Lys 
        115                 120                 125             


Glu Lys Met Glu Phe Val Phe Met Asp Ser Phe His Glu Met Leu Ser 
    130                 135                 140                 


Asp Phe Ala Lys His Gln Pro Gln Leu Ile Ala Asn Gly Lys Lys Val 
145                 150                 155                 160 


Pro Leu Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln 
                165                 170                 175     


Leu Ile Ser Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Lys 
            180                 185                 190         


Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu Gln Pro 
        195                 200                 205             


Thr Lys Lys Glu Glu Tyr Lys Val Leu Lys Val Ile Ser Val Asp Ala 
    210                 215                 220                 


Asp Glu Gly Gln Lys Gly Asn Gly Ile Trp Tyr Asn Ile His Leu Glu 
225                 230                 235                 240 


Asp Gly Gly Ile Tyr Lys Arg Lys Ser Asp Thr Pro Leu Tyr Asp Trp 
                245                 250                 255     


Val Gly Lys Thr Leu Gln Leu Ile Val Val Thr Ile Tyr Glu Lys Asp 
            260                 265                 270         


Gly Lys Thr Pro Lys Ile Phe Asp Ser Gly Lys Gln Asn Pro Ser Tyr 
        275                 280                 285             


Arg Leu Pro Lys Glu Asp Asp Trp Gly Leu Ile Lys Lys Arg Thr Glu 
    290                 295                 300                 


Ser Gln Ile Gln Ser Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Asp 
305                 310                 315                 320 


Asn Ile Leu Ser Lys Pro Asp Asp Lys Ile Arg Gly Asn Leu Val Arg 
                325                 330                 335     


Thr Ile Glu Arg Lys Tyr Tyr Lys Lys Glu Leu Ile Glu Ile Leu Gln 
            340                 345                 350         


Gln Gln Ala Lys Tyr His Asp Glu Leu His Asn Gln Asn Leu Leu Glu 
        355                 360                 365             


Ser Cys Ala Lys Glu Leu Tyr Ala His Asn Asp Val Arg Tyr Asn Glu 
    370                 375                 380                 


Val Val Lys Ser Asn Met Ile His Leu Leu Val Asp Asp Ile Ile Phe 
385                 390                 395                 400 


Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asn Cys Lys 
                405                 410                 415     


Tyr Glu Ser Tyr Glu Tyr Val Asp Lys Glu Thr Gly Glu Val Lys Glu 
            420                 425                 430         


Met Pro Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe 
        435                 440                 445             


Arg Leu Trp Gln Phe Ile His Asn Leu Arg Leu Ser Asp Val Val Thr 
    450                 455                 460                 


Arg Glu Asp Val Thr Ala Gln Tyr Leu Ser Thr Pro Gln Asp Tyr Cys 
465                 470                 475                 480 


Asn Leu Phe Thr Tyr Leu Asn Asp Arg Lys Glu Ile Lys Gln Asp Ile 
                485                 490                 495     


Leu Leu Lys Asp Phe Phe Lys Ile Lys Lys Val Gln Ile Asp Lys Glu 
            500                 505                 510         


Lys Val Phe Pro Ile Glu Trp Asn Tyr Ile Lys Asp Ala Thr Lys Thr 
        515                 520                 525             


Tyr Pro Cys Asn Glu Thr Arg Tyr Glu Leu Leu Ser Ala Leu Lys Arg 
    530                 535                 540                 


Ala Gly Met Asp Lys Gln Trp Leu Asp Asp Thr Pro Asn Met Gln Tyr 
545                 550                 555                 560 


Arg Leu Trp His Leu Leu Tyr Ser Val Glu Glu Gln Glu Glu Ser Ala 
                565                 570                 575     


Lys Ala Leu Arg Lys Leu Tyr Asp Asn Asp Asn Phe Val Ser Ser Phe 
            580                 585                 590         


Leu Lys Ile Lys Pro Phe Lys Lys Cys Tyr Gly Ala Tyr Ser Glu Lys 
        595                 600                 605             


Ala Ile Lys Arg Leu Leu Thr Leu Met Arg Met Gly Lys Ala Trp Asp 
    610                 615                 620                 


Glu Gln Ala Ile Asp Ala Lys Thr Leu Lys Cys Ile Gln Gln Ile Ile 
625                 630                 635                 640 


Thr Gly Asn Ile Asp Ser Lys Leu Lys Glu Lys Ile Asp Asp Ser Arg 
                645                 650                 655     


Tyr Val Leu His His Val Ser Asp Phe Gln Gly Leu Pro Thr Tyr Leu 
            660                 665                 670         


Ala Ser Tyr Val Ile Tyr Gly Arg His Ser Glu Val Thr Asp Ile Arg 
        675                 680                 685             


Cys Trp Asp Thr Pro Lys Asp Leu Leu Lys Phe Ile Cys Gly Phe Pro 
    690                 695                 700                 


Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Leu Glu Thr 
705                 710                 715                 720 


Leu Arg Val Val Tyr Asp Ile Trp Lys Ala Glu Lys His Ile Asp Glu 
                725                 730                 735     


Ile His Val Glu Val Ala Arg Glu Met Lys Leu Thr Ala Gln Gln Arg 
            740                 745                 750         


Asn Glu Ala Asn Leu Arg Asn Leu Asn Asn Glu Ala Thr Asn Leu Arg 
        755                 760                 765             


Ile Lys Tyr Leu Leu Glu Glu Leu Lys Asn Asp Thr Phe Ile Lys Asp 
    770                 775                 780                 


Val Arg Pro Leu Ser Pro Met His Gln Glu Lys Met Arg Ile Tyr Glu 
785                 790                 795                 800 


Gln Ala Val Leu Gly Asn Leu Asn Lys Gln Asp Lys Asp Tyr Asp Asp 
                805                 810                 815     


Ile Ile Lys Ile Ser Lys Lys Glu Arg Pro Thr Ser Ala Glu Leu Ile 
            820                 825                 830         


Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Cys Ser Pro Tyr Thr Gly 
        835                 840                 845             


Lys Ala Ile Ser Leu Ser Lys Leu Phe Thr Thr Ala Tyr Gln Ile Glu 
    850                 855                 860                 


His Val Ile Pro Gln Ser Arg Tyr Phe Asp Asn Ser Phe Asn Asn Lys 
865                 870                 875                 880 


Val Ile Cys Glu Ser Glu Val Asn Lys Glu Lys Asp Lys Met Leu Gly 
                885                 890                 895     


Tyr Glu Phe Ile Lys Gly Arg Gly Gly Asp Arg Ile Tyr Cys Thr Ala 
            900                 905                 910         


His Gly Lys Glu Val Thr Ile Leu Lys Glu Glu Glu Tyr Lys Gln Phe 
        915                 920                 925             


Val Asn Glu His Tyr Ala His Asn Lys Arg Lys Arg Asp Asn Leu Leu 
    930                 935                 940                 


Ala Glu Asn Ile Pro Glu Ser Phe Ser Ser Arg Gln Leu Asn Asp Thr 
945                 950                 955                 960 


Arg Tyr Ile Thr Lys Thr Ile Met Ser Leu Leu Ser Asn Val Val Arg 
                965                 970                 975     


Gln Gln Gly Glu Glu Glu Ala Thr Ala Lys Asn Leu Leu Pro Ser Ser 
            980                 985                 990         


Gly Ala Val Thr Asp Lys Leu Lys  Asn Asp Trp Gly Leu  Lys Asp Val 
        995                 1000                 1005             


Trp Asn  Thr Ile Val Thr Pro  Arg Phe Glu Arg Leu  Asn Lys Leu 
    1010                 1015                 1020             


Ser Gly  Thr Asn Asp Phe Gly  Glu Met Lys Glu Glu  Asn Gly Asn 
    1025                 1030                 1035             


Arg Tyr  Phe Gln Thr Asn Val  Pro Ser Glu Tyr Glu  Lys Gly Phe 
    1040                 1045                 1050             


Lys Lys  Lys Arg Ile Asp His  Arg His His Ala Met  Asp Ala Leu 
    1055                 1060                 1065             


Val Ile  Ala Cys Thr Thr Arg  Asn Met Val Asn Tyr  Ile Ser Asn 
    1070                 1075                 1080             


Val Asn  Ala Asn Ser Pro Lys  Gln Arg Glu Asp Leu  Arg Gln Leu 
    1085                 1090                 1095             


Leu Cys  Asp Lys Asn Arg Ile  Ile Asn Lys Pro Trp  Asn Thr Phe 
    1100                 1105                 1110             


Thr Gln  Asp Ala Leu Lys Ala  Leu Asn Asp Ile Val  Val Ser Phe 
    1115                 1120                 1125             


Lys Asn  Asn Val Arg Ile Ile  Asn Arg Ala Thr Asn  Arg Tyr Gln 
    1130                 1135                 1140             


Arg Tyr  Asp Lys Asn Gly Lys  Lys Met Ile Cys Ser  Gln Lys Gly 
    1145                 1150                 1155             


Asn Asp  Met Trp Ala Ile Arg  Lys Ser Met His Lys  Glu Thr Val 
    1160                 1165                 1170             


Phe Gly  Arg Val Asn Leu Ile  Arg Lys Glu Ala Leu  Pro Ile Ala 
    1175                 1180                 1185             


Lys Ala  Leu Asp Asn Ile Ser  Ala Ile Cys Asn Ala  Gln Leu Arg 
    1190                 1195                 1200             


Ala Tyr  Val Asn Asp Leu Val  Asp Lys His Phe Asn  Lys Lys Gln 
    1205                 1210                 1215             


Leu Val  Ala His Phe Lys Ser  Leu Asp Tyr Lys Trp  Asn Arg Gln 
    1220                 1225                 1230             


Asp Val  Ser Lys Val Glu Val  Trp Ile Ser Ser Asp  Asp Lys Thr 
    1235                 1240                 1245             


Pro Met  Val Ala Val Arg Lys  Pro Leu Asp Thr Ser  Phe Asp Lys 
    1250                 1255                 1260             


Lys Lys  Ile Ala Ser Ile Thr  Asp Thr Gly Ile Gln  Ala Ile Leu 
    1265                 1270                 1275             


Leu Asn  Tyr Leu Glu Ser Lys  Gly Gly Asp Ala Leu  Val Ala Phe 
    1280                 1285                 1290             


Ala Pro  Glu Gly Ile Gln Glu  Met Asn Ser Asn Ile  Glu Leu Tyr 
    1295                 1300                 1305             


Asn Asn  Gly Lys Ala His Lys  Pro Ile Lys Asn Val  Arg Leu Ser 
    1310                 1315                 1320             


Glu Thr  Leu Gly Ala Lys Tyr  Gln Val Gly Glu Arg  Gly Ala Lys 
    1325                 1330                 1335             


Thr Lys  Lys Tyr Val Val Ala  Asp Lys Gly Thr Asn  Leu Phe Phe 
    1340                 1345                 1350             


Ala Ile  Tyr Glu Asp Glu Glu  Gly Lys Arg Tyr Tyr  Tyr Ser Val 
    1355                 1360                 1365             


Pro Leu  Arg Glu Ala Ile Glu  Arg Gln Lys Gln Lys  Leu Ser Pro 
    1370                 1375                 1380             


Val Pro  Glu Tyr Asn Glu Lys  Gly Val Ala Leu Lys  Phe Tyr Leu 
    1385                 1390                 1395             


Ser Pro  Asn Asp Leu Val Tyr  Val Pro Thr Glu Glu  Glu Arg Thr 
    1400                 1405                 1410             


Met Gly  Thr Cys His Ile Asp  Lys Thr Arg Ile Tyr  Lys Met Val 
    1415                 1420                 1425             


Ser Ser  Ser Glu Asn Arg Cys  Cys Phe Val Pro Ser  Thr Ile Ala 
    1430                 1435                 1440             


Lys Val  Ile Ala Asp Lys Val  Glu Leu Leu Ser His  Asn Lys Ile 
    1445                 1450                 1455             


Glu Lys  Ala Ile Thr Gly Glu  Ser Ile Lys Glu Val  Cys Trp Lys 
    1460                 1465                 1470             


Leu Lys  Val Asp Arg Leu Gly  Asn Ile Leu Asn Val  Ile Lys 
    1475                 1480                 1485         


<210>  1295
<211>  1487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1295

Met Lys Asn Val Leu Gly Leu Asp Leu Gly Ser Asn Ser Val Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Thr Glu Asp Asn Gly Asn Pro Ile Gly Ser Ile Lys 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Ser Phe 
        35                  40                  45              


Glu Lys Gly Asp Thr Val Ser Gln Thr Ala Gln Arg Thr Leu Tyr Arg 
    50                  55                  60                  


Gly Lys Arg Arg Leu Thr Glu Arg His Val Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


Cys Arg Val Leu His Ile Met Asn Phe Leu Pro Asp His Phe Asp Lys 
                85                  90                  95      


Leu Leu Gly Trp Asp Lys Thr Asp Asn Lys Thr Tyr Gly Lys Phe Ile 
            100                 105                 110         


Asp Asp Ser Glu Pro Lys Leu Ala Trp Arg Gln Asn Lys Asn Glu Lys 
        115                 120                 125             


Glu Lys Met Glu Phe Val Phe Met Asp Ser Phe His Glu Met Leu Ser 
    130                 135                 140                 


Asp Phe Ala Lys His Gln Pro Gln Leu Ile Ala Asn Gly Lys Lys Val 
145                 150                 155                 160 


Pro Leu Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln 
                165                 170                 175     


Leu Ile Ser Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Lys 
            180                 185                 190         


Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu Gln Pro 
        195                 200                 205             


Thr Lys Lys Glu Glu Tyr Lys Val Leu Lys Val Ile Ser Val Asp Ala 
    210                 215                 220                 


Asp Glu Gly Gln Lys Gly Asn Gly Ile Trp Tyr Asn Ile His Leu Glu 
225                 230                 235                 240 


Asp Gly Gly Ile Tyr Lys Arg Lys Ser Asp Ile Pro Leu Tyr Asp Trp 
                245                 250                 255     


Val Gly Lys Thr Leu Gln Leu Ile Val Val Thr Ile Tyr Glu Lys Asp 
            260                 265                 270         


Gly Lys Thr Pro Lys Ile Phe Asp Ser Gly Lys Gln Asn Pro Ser Tyr 
        275                 280                 285             


Arg Leu Pro Lys Glu Asp Asp Trp Gly Leu Ile Lys Lys Arg Thr Glu 
    290                 295                 300                 


Ser Gln Ile Gln Ser Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Asp 
305                 310                 315                 320 


Asn Ile Leu Ser Lys Pro Ala Asp Lys Ile Arg Gly Asn Leu Val Arg 
                325                 330                 335     


Thr Ile Glu Arg Lys Tyr Tyr Lys Lys Glu Leu Ile Glu Ile Leu Gln 
            340                 345                 350         


Gln Gln Ala Lys Tyr His Asp Glu Leu His Asn Gln His Leu Leu Glu 
        355                 360                 365             


Ser Cys Ala Lys Glu Leu Tyr Ala His Asn Asp Val Arg Tyr Asn Glu 
    370                 375                 380                 


Val Val Lys Ser Asn Met Ile His Leu Leu Val Asp Asp Ile Ile Phe 
385                 390                 395                 400 


Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Asp Asn Cys Lys 
                405                 410                 415     


Tyr Glu Ser Tyr Glu Tyr Val Asp Lys Glu Thr Gly Glu Val Lys Glu 
            420                 425                 430         


Met Pro Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe 
        435                 440                 445             


Arg Leu Trp Gln Phe Ile His Asn Leu Arg Leu Ser Asp Val Val Thr 
    450                 455                 460                 


Arg Glu Asp Val Thr Ala Gln Tyr Leu Ser Thr Pro Gln Asp Tyr Cys 
465                 470                 475                 480 


Asn Leu Phe Thr Tyr Leu Asn Asp Arg Lys Glu Ile Lys Gln Asp Ile 
                485                 490                 495     


Leu Leu Lys Asp Phe Phe Gln Ile Lys Lys Val Gln Ile Asp Lys Glu 
            500                 505                 510         


Lys Val Phe Pro Ile Glu Trp Asn Tyr Ile Lys Asp Ala Thr Lys Thr 
        515                 520                 525             


Tyr Pro Cys Asn Glu Thr Lys Tyr Glu Leu Leu Ser Ala Leu Lys Arg 
    530                 535                 540                 


Ala Gly Met Asp Lys Gln Trp Leu Asp Asp Thr Pro Asn Met Gln Tyr 
545                 550                 555                 560 


Arg Leu Trp His Leu Leu Tyr Ser Val Glu Glu Lys Glu Glu Ser Ala 
                565                 570                 575     


Lys Ala Leu Arg Lys Leu Tyr Asp Asp Asp Asn Phe Val Ser Ser Phe 
            580                 585                 590         


Leu Lys Ile Lys Pro Phe Glu Lys Cys Tyr Gly Ala Tyr Ser Glu Lys 
        595                 600                 605             


Ala Ile Lys Arg Leu Leu Thr Leu Met Arg Met Gly Lys Ala Trp Asp 
    610                 615                 620                 


Glu Gln Ala Ile Asp Ala Lys Thr Leu Lys Cys Ile Gln Gln Ile Ile 
625                 630                 635                 640 


Thr Gly Asn Ile Asp Ser Lys Leu Lys Glu Lys Ile Asp Asp Ser Arg 
                645                 650                 655     


Tyr Val Leu His His Val Ser Asp Phe Gln Gly Leu Pro Thr Tyr Leu 
            660                 665                 670         


Ala Ser Tyr Val Ile Tyr Gly Arg His Ser Glu Val Thr Asp Ile Arg 
        675                 680                 685             


Cys Trp Asp Thr Pro Lys Asp Leu Leu Lys Phe Ile Cys Gly Phe Pro 
    690                 695                 700                 


Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Leu Glu Thr 
705                 710                 715                 720 


Leu Arg Val Val Tyr Asp Ile Trp Lys Ala Glu Lys His Ile Asp Glu 
                725                 730                 735     


Ile His Val Glu Val Ala Arg Glu Met Lys Leu Thr Ala Gln Gln Arg 
            740                 745                 750         


Asn Glu Ala Asn Leu Arg Asn Leu Asn Asn Glu Ala Thr Asn Leu Arg 
        755                 760                 765             


Ile Lys Tyr Leu Leu Glu Glu Leu Lys Asn Asp Thr Phe Ile Lys Asp 
    770                 775                 780                 


Val Arg Pro Leu Ser Pro Met His Gln Glu Lys Met Arg Ile Tyr Glu 
785                 790                 795                 800 


Gln Ala Val Leu Gly Asn Leu Asn Lys Gln Asp Lys Asp Tyr Asp Asp 
                805                 810                 815     


Ile Ile Lys Ile Ser Lys Lys Glu Arg Pro Thr Ser Ala Glu Leu Ile 
            820                 825                 830         


Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Cys Ser Pro Tyr Thr Gly 
        835                 840                 845             


Lys Ala Ile Ser Leu Ser Lys Leu Phe Thr Thr Ala Tyr Gln Ile Glu 
    850                 855                 860                 


His Val Ile Pro Gln Ser Arg Tyr Phe Asp Asn Ser Phe Asn Asn Lys 
865                 870                 875                 880 


Val Ile Cys Glu Ser Glu Val Asn Lys Glu Lys Asp Lys Met Leu Gly 
                885                 890                 895     


Tyr Glu Phe Ile Lys Gly Arg Gly Gly Asp Arg Ile Tyr Cys Thr Ala 
            900                 905                 910         


His Gly Lys Glu Val Thr Ile Leu Lys Glu Glu Glu Tyr Lys Gln Phe 
        915                 920                 925             


Val Asn Glu His Tyr Ala His Asn Lys Arg Lys Arg Asp Asn Leu Leu 
    930                 935                 940                 


Ala Glu Asn Ile Pro Glu Ser Phe Ser Ser Arg Gln Leu Asn Asp Thr 
945                 950                 955                 960 


Arg Tyr Ile Thr Lys Thr Ile Met Ser Leu Leu Ser Asn Val Val Arg 
                965                 970                 975     


Gln Gln Gly Glu Glu Glu Ala Thr Ala Lys Asn Leu Leu Pro Ser Ser 
            980                 985                 990         


Gly Ala Val Thr Asp Lys Leu Lys  Asn Asp Trp Gly Leu  Lys Asp Val 
        995                 1000                 1005             


Trp Asn  Thr Ile Val Thr Pro  Arg Phe Glu Arg Leu  Asn Lys Leu 
    1010                 1015                 1020             


Ser Gly  Thr Asn Asp Phe Gly  Glu Met Lys Glu Glu  Asn Gly Asn 
    1025                 1030                 1035             


Arg Tyr  Phe Gln Thr Asn Val  Pro Ser Glu Tyr Glu  Lys Gly Phe 
    1040                 1045                 1050             


Lys Lys  Lys Arg Ile Asp His  Arg His His Ala Met  Asp Ala Leu 
    1055                 1060                 1065             


Val Ile  Ala Cys Thr Thr Arg  Asn Met Val Asn Tyr  Ile Ser Asn 
    1070                 1075                 1080             


Val Asn  Ala Asn Ser Pro Lys  Gln Arg Glu Asp Leu  Arg Gln Leu 
    1085                 1090                 1095             


Leu Cys  Asp Lys Asn Arg Ile  Ile Asn Lys Pro Trp  Asn Thr Phe 
    1100                 1105                 1110             


Thr Gln  Asp Ala Leu Lys Ala  Leu Asn Asp Ile Val  Val Ser Phe 
    1115                 1120                 1125             


Lys Asn  Asn Val Arg Ile Ile  Asn Arg Ala Thr Asn  Arg Tyr Gln 
    1130                 1135                 1140             


Arg Tyr  Asp Lys Asn Gly Lys  Lys Met Ile Cys Ser  Gln Lys Gly 
    1145                 1150                 1155             


Asn Asp  Met Trp Ala Ile Arg  Lys Ser Met His Lys  Glu Thr Val 
    1160                 1165                 1170             


Phe Gly  Arg Val Asn Leu Ile  Arg Lys Glu Ala Leu  Pro Ile Ala 
    1175                 1180                 1185             


Lys Ala  Leu Asp Asn Ile Ser  Ala Ile Cys Asn Ala  Gln Leu Arg 
    1190                 1195                 1200             


Ala Tyr  Val Asn Asp Leu Val  Asp Lys His Phe Asn  Lys Lys Gln 
    1205                 1210                 1215             


Leu Val  Ala His Phe Lys Ser  Leu Asp Tyr Lys Trp  Asn Arg Gln 
    1220                 1225                 1230             


Asp Val  Ser Lys Val Glu Val  Trp Ile Ser Ser Asp  Asp Lys Thr 
    1235                 1240                 1245             


Pro Met  Val Ala Val Arg Lys  Pro Leu Asp Thr Ser  Phe Asp Lys 
    1250                 1255                 1260             


Lys Lys  Ile Ala Ser Ile Thr  Asp Thr Gly Ile Gln  Ala Ile Leu 
    1265                 1270                 1275             


Leu Asn  Tyr Leu Glu Ser Lys  Gly Gly Asp Ala Leu  Val Ala Phe 
    1280                 1285                 1290             


Ala Pro  Glu Gly Ile Gln Glu  Met Asn Ser Asn Ile  Glu Leu Tyr 
    1295                 1300                 1305             


Asn Asn  Gly Lys Ala His Lys  Pro Ile Lys Asn Val  Arg Leu Ser 
    1310                 1315                 1320             


Glu Thr  Leu Gly Ala Lys Tyr  Gln Val Gly Glu Arg  Gly Ala Lys 
    1325                 1330                 1335             


Thr Lys  Lys Tyr Val Val Ala  Asp Lys Gly Thr Asn  Leu Phe Phe 
    1340                 1345                 1350             


Ala Ile  Tyr Glu Asp Glu Glu  Gly Lys Arg Tyr Tyr  Tyr Ser Val 
    1355                 1360                 1365             


Pro Leu  Arg Glu Ala Ile Glu  Arg Gln Lys Gln Lys  Leu Ser Pro 
    1370                 1375                 1380             


Val Pro  Glu Tyr Asn Glu Lys  Gly Val Ala Leu Lys  Phe Tyr Leu 
    1385                 1390                 1395             


Ser Pro  Asn Asp Leu Val Tyr  Val Pro Thr Glu Glu  Glu Arg Thr 
    1400                 1405                 1410             


Met Gly  Thr Cys His Ile Asp  Lys Thr Arg Ile Tyr  Lys Met Val 
    1415                 1420                 1425             


Ser Ser  Ser Glu Asn Arg Cys  Cys Phe Val Pro Ser  Thr Ile Ala 
    1430                 1435                 1440             


Lys Val  Ile Ala Asp Lys Val  Glu Leu Leu Ser His  Asn Lys Ile 
    1445                 1450                 1455             


Glu Lys  Ala Ile Thr Gly Glu  Ser Ile Lys Glu Val  Cys Trp Lys 
    1460                 1465                 1470             


Leu Lys  Val Asp Arg Leu Gly  Asn Ile Leu Asn Val  Ile Lys 
    1475                 1480                 1485         


<210>  1296
<211>  1487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1296

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Ser Asn Ser Val Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Val Glu Glu Asn Gly Asn Pro Ile Gly Ser Ile Lys 
            20                  25                  30          


Met Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Ser Phe 
        35                  40                  45              


Glu Lys Gly Asp Thr Val Ser Gln Thr Ala Gln Arg Thr Leu Tyr Arg 
    50                  55                  60                  


Gly Lys Arg Arg Leu Ile Glu Arg His Val Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


Cys Arg Val Leu His Ile Met Asn Phe Leu Pro Ala His Phe Asp Arg 
                85                  90                  95      


Leu Leu Gly Trp Asp Lys Thr Asp Asn Lys Thr Tyr Gly Lys Phe Ile 
            100                 105                 110         


Asp Asp Ser Glu Pro Lys Leu Ala Trp Arg Gln Asn Lys Asn Glu Met 
        115                 120                 125             


Gly Lys Met Glu Phe Val Phe Met Asp Ser Phe His Glu Met Leu Ser 
    130                 135                 140                 


Asp Phe Ala Lys His Gln Pro Gln Leu Ile Ala Asn Gly Lys Lys Val 
145                 150                 155                 160 


Pro Leu Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln 
                165                 170                 175     


Leu Ile Ser Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Lys 
            180                 185                 190         


Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu Gln Pro 
        195                 200                 205             


Thr Lys Lys Glu Glu Tyr Lys Val Leu Lys Val Ile Ser Val Asp Ala 
    210                 215                 220                 


Asp Glu Gly Gln Lys Gly Asn Gly Ile Trp Tyr Asn Ile His Leu Glu 
225                 230                 235                 240 


Asp Gly Gly Ile Tyr Lys Lys Lys Ser Asp Ile Pro Leu Tyr Asp Trp 
                245                 250                 255     


Val Gly Lys Thr Leu Gln Leu Ile Val Val Thr Thr Tyr Glu Lys Asp 
            260                 265                 270         


Gly Lys Thr Pro Lys Ile Phe Asp Ser Gly Lys Gln Asn Pro Ser Tyr 
        275                 280                 285             


Arg Leu Pro Lys Glu Asp Asp Trp Gly Leu Val Lys Lys Arg Thr Glu 
    290                 295                 300                 


Ser Gln Leu Gln Ser Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Asp 
305                 310                 315                 320 


Asn Ile Leu Ser Lys Pro Asp Asp Lys Ile Arg Gly Asn Leu Val Arg 
                325                 330                 335     


Thr Ile Glu Arg Lys Tyr Tyr Lys Lys Glu Leu Ile Glu Ile Leu Gln 
            340                 345                 350         


Gln Gln Ala Lys Tyr His Asp Glu Leu His Asn Gln Asn Leu Leu Glu 
        355                 360                 365             


Ser Cys Ala Lys Glu Leu Tyr Ala His Asn Asp Val His Tyr Asn Glu 
    370                 375                 380                 


Val Ile Lys Ser Asn Met Ile Asn Leu Leu Val Asn Asp Ile Ile Phe 
385                 390                 395                 400 


Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asn Cys Lys 
                405                 410                 415     


Tyr Glu Ser Tyr Glu Tyr Val Asp Lys Glu Thr Gly Glu Ile Lys Glu 
            420                 425                 430         


Met Pro Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe 
        435                 440                 445             


Arg Leu Trp Gln Phe Ile His Asn Leu Arg Leu Ser Asp Val Val Thr 
    450                 455                 460                 


Arg Glu Asp Val Thr Ala Gln Tyr Leu Ser Thr Pro Gln Asp Tyr Cys 
465                 470                 475                 480 


Asn Leu Phe Thr Tyr Leu Asn Asp Arg Lys Glu Ile Lys Gln Asp Ile 
                485                 490                 495     


Leu Leu Lys Asp Phe Phe Lys Ile Lys Lys Val Gln Ile Asp Lys Glu 
            500                 505                 510         


Lys Val Phe Pro Ile Glu Trp Asn Tyr Ile Lys Asp Ala Thr Lys Ser 
        515                 520                 525             


Tyr Pro Cys Asn Glu Thr Arg Tyr Glu Leu Leu Ser Ala Leu Lys Arg 
    530                 535                 540                 


Ala Gly Met Asp Lys Gln Trp Leu Asp Asp Thr Pro Asn Met Gln Tyr 
545                 550                 555                 560 


Arg Leu Trp His Leu Leu Tyr Ser Val Glu Glu Lys Glu Glu Ser Ala 
                565                 570                 575     


Lys Ala Leu Arg Lys Leu Tyr Asp Asp Asp Lys Phe Val Thr Ser Phe 
            580                 585                 590         


Leu Lys Ile Lys Pro Phe Asn Lys Cys Tyr Gly Ala Tyr Ser Glu Lys 
        595                 600                 605             


Ala Ile Lys Arg Leu Leu Thr Leu Met Arg Met Gly Lys Ala Trp Asn 
    610                 615                 620                 


Glu Gln Ala Ile Asp Ala Lys Thr Leu Lys Cys Ile Gln Gln Ile Ile 
625                 630                 635                 640 


Thr Asp Asn Val Asp Ser Lys Leu Lys Glu Lys Ile Asp Asp Ser Arg 
                645                 650                 655     


Tyr Val Leu His His Val Ser Asp Phe Gln Gly Leu Pro Thr Tyr Leu 
            660                 665                 670         


Ala Ser Tyr Val Ile Tyr Gly Arg His Ser Glu Val Thr Asp Ile Gln 
        675                 680                 685             


Cys Trp Asp Ser Pro Glu Asp Leu Leu Lys Phe Ile Arg Asp Phe Lys 
    690                 695                 700                 


Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Leu Glu Thr 
705                 710                 715                 720 


Leu Arg Val Val Tyr Asp Ile Trp Lys Ala Glu Lys His Ile Asp Glu 
                725                 730                 735     


Ile His Val Glu Val Ala Arg Glu Met Lys Leu Thr Ala Gln Gln Arg 
            740                 745                 750         


Asn Glu Ala Asn Leu Arg Asn Leu Asn Asn Glu Ala Thr Asn Leu Arg 
        755                 760                 765             


Ile Lys Tyr Leu Leu Glu Glu Leu Lys Asn Asp Thr Phe Ile Lys Asp 
    770                 775                 780                 


Val Arg Pro Leu Ser Pro Ile His Gln Glu Lys Met Arg Ile Tyr Glu 
785                 790                 795                 800 


Gln Ala Val Leu Gly Asn Leu Asn Lys Gln Asp Lys Asp Tyr Asp Asp 
                805                 810                 815     


Ile Ile Lys Ile Ser Lys Lys Glu His Pro Thr Asn Ala Glu Leu Ile 
            820                 825                 830         


Arg Tyr Lys Leu Trp Leu Glu Gln Arg Tyr Cys Ser Pro Tyr Thr Gly 
        835                 840                 845             


Lys Ala Ile Ser Leu Ser Lys Leu Phe Thr Thr Ala Tyr Glu Ile Glu 
    850                 855                 860                 


His Ile Ile Pro Gln Ser Arg Tyr Phe Asp Asn Ser Phe Asn Asn Lys 
865                 870                 875                 880 


Val Ile Cys Glu Ala Glu Val Asn Lys Ala Lys Gly Asn Met Leu Gly 
                885                 890                 895     


Tyr Glu Phe Ile Lys Lys Gln Gly Gly Gln Lys Ile Tyr Cys Thr Gln 
            900                 905                 910         


Leu Gly Lys Ala Val Thr Ile Leu Lys Glu Glu Ala Tyr Lys Gln Phe 
        915                 920                 925             


Val Asn Glu His Tyr Ala His Asn Lys Arg Lys Arg Asp Asn Leu Leu 
    930                 935                 940                 


Ala Glu Asn Ile Pro Glu Ser Phe Ser Ser Arg Gln Leu Asn Asp Thr 
945                 950                 955                 960 


Arg Tyr Ile Thr Lys Thr Ile Met Ser Leu Leu Ser Asn Val Val Arg 
                965                 970                 975     


Gln Gln Ser Glu Glu Glu Ala Thr Ala Lys Asn Leu Leu Pro Ser Ser 
            980                 985                 990         


Gly Ser Val Thr Asp Arg Leu Lys  Asn Asp Trp Gly Leu  Lys Asp Val 
        995                 1000                 1005             


Trp Asn  Thr Ile Val Thr Pro  Arg Phe Glu Arg Leu  Asn Lys Leu 
    1010                 1015                 1020             


Ser Gly  Thr Asn Asp Phe Gly  Glu Met Lys Glu Glu  Asn Gly Asn 
    1025                 1030                 1035             


Arg Tyr  Phe Gln Thr Asn Val  Pro Leu Glu Tyr Glu  Lys Gly Phe 
    1040                 1045                 1050             


Lys Lys  Lys Arg Ile Asp His  Arg His His Ala Met  Asp Ala Leu 
    1055                 1060                 1065             


Val Ile  Ala Cys Thr Thr Arg  Asn Met Val Asn Tyr  Ile Ser Asn 
    1070                 1075                 1080             


Ala Asn  Ala Asn Ser Pro Lys  Gln Arg Glu Asp Leu  Arg Gln Leu 
    1085                 1090                 1095             


Leu Cys  Asp Lys Asn Arg Ile  Ile Asn Lys Pro Trp  Asp Thr Phe 
    1100                 1105                 1110             


Thr Gln  Asp Ala Leu Lys Ala  Leu Asn Asp Ile Val  Val Ser Phe 
    1115                 1120                 1125             


Lys Asn  Asn Val Arg Ile Ile  Asn Arg Ala Thr Asn  Arg Tyr Gln 
    1130                 1135                 1140             


Arg Tyr  Asp Lys Asn Gly Lys  Lys Ile Ile Phe Ser  Gln Lys Gly 
    1145                 1150                 1155             


Asp Asp  Met Trp Ala Ile Arg  Lys Ser Met His Lys  Glu Thr Val 
    1160                 1165                 1170             


Phe Gly  Arg Val Asn Leu Ile  Arg Lys Glu Val Leu  Pro Ile Ala 
    1175                 1180                 1185             


Lys Ala  Leu Asp Asn Ile Ser  Ala Ile Cys Asn Ala  Gln Leu Arg 
    1190                 1195                 1200             


Ala Tyr  Val Asn Asp Leu Val  Asp Lys His Phe Asn  Lys Lys Gln 
    1205                 1210                 1215             


Leu Val  Ala His Phe Lys Ser  Leu Asn Tyr Lys Trp  Asn Arg Gln 
    1220                 1225                 1230             


Asp Val  Ser Lys Val Glu Val  Trp Ile Ser Ser Asp  Asn Lys Thr 
    1235                 1240                 1245             


Pro Met  Val Ala Val Arg Lys  Pro Leu Asp Thr Ser  Phe Asp Lys 
    1250                 1255                 1260             


Lys Lys  Ile Ala Ser Ile Thr  Asp Thr Gly Ile Gln  Thr Ile Leu 
    1265                 1270                 1275             


Leu Asn  Tyr Leu Glu Ser Lys  Gly Gly Asp Ala Ser  Val Ala Phe 
    1280                 1285                 1290             


Ala Pro  Glu Gly Ile Gln Glu  Met Asn Ser Asn Ile  Glu Leu Tyr 
    1295                 1300                 1305             


Asn Asn  Gly Lys Ala His Lys  Pro Ile Lys Asn Val  Arg Leu Ser 
    1310                 1315                 1320             


Glu Pro  Leu Gly Ala Lys Tyr  Gln Val Gly Glu Arg  Gly Ala Lys 
    1325                 1330                 1335             


Thr Lys  Lys Tyr Val Glu Ala  Asp Lys Gly Thr Asn  Leu Phe Phe 
    1340                 1345                 1350             


Ala Ile  Tyr Glu Asp Glu Glu  Gly Lys Arg Tyr Tyr  Tyr Ser Val 
    1355                 1360                 1365             


Pro Leu  Arg Glu Ala Ile Glu  Arg Gln Lys Gln Lys  Leu Ser Pro 
    1370                 1375                 1380             


Val Pro  Glu Tyr Asn Glu Lys  Gly Val Ala Leu Lys  Phe Tyr Leu 
    1385                 1390                 1395             


Ser Pro  Asn Asp Leu Val Tyr  Val Pro Thr Glu Glu  Glu Arg Thr 
    1400                 1405                 1410             


Met Gly  Thr Cys His Ile Asp  Lys Ala Arg Ile Tyr  Lys Met Val 
    1415                 1420                 1425             


Ser Ser  Thr Gly Cys Ser Cys  Tyr Phe Val Pro Ser  Thr Phe Ala 
    1430                 1435                 1440             


Lys Val  Ile Val Asp Lys Val  Glu Leu Gln Ser Leu  Asn Lys Met 
    1445                 1450                 1455             


Glu Lys  Ala Ile Thr Asp Glu  Cys Ile Lys Glu Val  Cys Trp Lys 
    1460                 1465                 1470             


Leu Lys  Val Asp Arg Leu Gly  Asn Ile Ile Asn Val  Ile Lys 
    1475                 1480                 1485         


<210>  1297
<211>  1494
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1297

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Asp Asn Glu Ser His Arg Ile Ile Lys Ser Gly Ser Arg 
            20                  25                  30          


Ile Ile Pro Met Asp Ala Ala Thr Met Gly Asp Tyr Glu Lys Gly Asn 
        35                  40                  45              


Leu Lys Ser Ala Asp Trp Lys Arg Thr Ser Phe Arg Gly Met Arg Arg 
    50                  55                  60                  


Leu Tyr Glu Arg Ala Lys Leu Arg Arg Glu Arg Leu Leu Arg Val Leu 
65                  70                  75                  80  


Asn Ile Leu Gly Phe Leu Pro Glu Glu Phe Arg Arg Gln Ile Asp Phe 
                85                  90                  95      


Glu His His Pro Gly Gln Phe Ile Asp Gly Lys Glu Pro Leu Ile Ala 
            100                 105                 110         


Tyr His His Asp Glu Asn Gly Gln Arg Val Phe Ser Tyr Met Ser Ala 
        115                 120                 125             


Phe Glu Glu Met Leu Thr Asp Phe Arg Ala Thr Gln Ser Glu Leu Val 
    130                 135                 140                 


Arg Asp Gly Arg Arg Val Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg 
145                 150                 155                 160 


His Lys Ala Leu Thr Gln Pro Val Thr Lys Glu Glu Leu Ala Trp Ile 
                165                 170                 175     


Ile Leu Asn Phe Asn Thr Lys Arg Gly Tyr Tyr Gln Leu Arg Ser Glu 
            180                 185                 190         


Ser Glu Glu Val Thr Thr Asn Lys Asn Glu Glu Tyr Ala Glu Leu Lys 
        195                 200                 205             


Val Val Ser Val Glu Lys Thr Gly Glu Asp Lys Lys Arg Ala Gly Tyr 
    210                 215                 220                 


Tyr Trp Tyr Thr Ile Thr Tyr Asp Asn Glu Ala Glu Gln Lys Ile Thr 
225                 230                 235                 240 


Ser Thr Ile Pro Pro Arg Gln Ile Gly Asp Lys Val Glu Leu Ile Val 
                245                 250                 255     


Thr Thr Ala Arg Asp Lys Ala Gly Glu Ile Lys Lys Ser Val Arg Ser 
            260                 265                 270         


Pro Lys Glu Asp Asp Trp Thr Leu Met Lys Lys Arg Thr Glu His Asn 
        275                 280                 285             


Ile Glu Glu Glu Glu Cys Thr Val Gly Ser Tyr Ile Tyr Gln His Ile 
    290                 295                 300                 


Leu Ala Asp Pro Thr Val Lys Val Arg Gly Lys Leu Val His Thr Ile 
305                 310                 315                 320 


Glu Arg Lys Phe Tyr Lys Lys Glu Leu Lys Gln Ile Leu Asn Lys Gln 
                325                 330                 335     


Lys Glu Phe Ile Pro Glu Leu Gln Asp Ala Ser Leu Tyr Glu Ala Cys 
            340                 345                 350         


Ile Lys Glu Leu Tyr Arg Asn Asn Glu Ala His Ile Gln Ser Ile Ala 
        355                 360                 365             


Asn Lys Asp Phe Thr Asp Leu Phe Val Asn Asp Ile Ile Phe Tyr Gln 
    370                 375                 380                 


Arg Pro Leu Lys Ser Lys Lys Ser Thr Ile Ser Asn Cys Pro Tyr Glu 
385                 390                 395                 400 


Ser Tyr Tyr Tyr Lys Asn Lys Glu Thr Gly Glu Leu Ile Arg Lys Pro 
                405                 410                 415     


Ile Lys Cys Ile Ala Lys Ser Asn Pro Leu Tyr Gln Glu Phe Arg Leu 
            420                 425                 430         


Trp Gln Phe Val His Asn Leu Arg Ile Tyr Lys Lys Ser Glu Glu Val 
        435                 440                 445             


Ser Gly Arg Leu Gln Thr Asp Val Asp Val Thr Asp Arg Phe Leu Thr 
    450                 455                 460                 


Thr Pro Asp Asp Tyr Ala Lys Leu Phe Ser Trp Leu Asn Asp Arg Lys 
465                 470                 475                 480 


Glu Ile Lys Gln Lys Glu Leu Leu Arg Tyr Glu Ala Phe Gly Leu Ser 
                485                 490                 495     


Lys Ser Tyr Gly Asp Tyr Arg Trp Asn Phe Val Glu Asp Lys Pro Tyr 
            500                 505                 510         


Pro Cys Asn Glu Thr Leu His Glu Ile Asn Arg Arg Met Lys Ser Ile 
        515                 520                 525             


Leu Ser Asp Lys Lys Leu Ser Ser Glu Ala Tyr Gln Gln Leu Trp His 
    530                 535                 540                 


Ile Leu Tyr Ser Ile Asp Asp Pro Ile Glu Ile Arg Lys Ala Leu Thr 
545                 550                 555                 560 


Ser Phe Ala Gln Asn Gln Glu Ile Asn Ser Asp Asp Phe Val Asn Ala 
                565                 570                 575     


Phe Tyr Gln Tyr Val Pro Glu Lys Thr Lys Ser Tyr Gly Ala Tyr Ser 
            580                 585                 590         


Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Arg Gly Ser Tyr 
        595                 600                 605             


Trp Ala Ile Gly Asn Ile Asp Glu Lys Thr His Arg Arg Leu Asp Asn 
    610                 615                 620                 


Ile Ile Asn Gly Val Ala Asp Asp Ser Ile Ser Thr Cys Thr Arg Glu 
625                 630                 635                 640 


Lys Leu Asn Asn Leu Thr Asp Ile Thr Met Cys Gln Gly Met Pro Leu 
                645                 650                 655     


Trp Gln Ala Cys Tyr Ala Val Tyr Asp Arg His Ser Glu Ala Ala Asp 
            660                 665                 670         


Thr Thr Lys Trp Glu Gln Pro Asp Asp Ile Asp Thr Tyr Leu Ser Thr 
        675                 680                 685             


Glu Leu Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Thr Val Ile 
    690                 695                 700                 


Ser Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Ala Tyr Gly Arg 
705                 710                 715                 720 


Pro Glu Glu Ile His Val Glu Leu Gly Arg Asn Leu Lys Gln Asp Ala 
                725                 730                 735     


Lys Ser Arg Lys Gln Asp Ala Ile Arg Met Ala Glu Asn Glu Gln Thr 
            740                 745                 750         


Asn Leu Arg Ile Arg Ala Leu Leu Gln Glu Leu Ser Asn Pro Glu Cys 
        755                 760                 765             


Gly Val Glu Asn Val Arg Pro His Ser Pro Ser Gln Met Glu Leu Leu 
    770                 775                 780                 


Lys Ile Tyr Glu Asn Glu Val Leu Gln Ser Thr Asp Asp Ile Pro Glu 
785                 790                 795                 800 


Glu Ile Ser Asn Ile Ile Lys Asp Leu Gly Lys Thr Asp Ser Lys Lys 
                805                 810                 815     


Arg Pro Ser Leu Ser Asp Ile Thr Lys Tyr Arg Leu Trp Leu Glu Gln 
            820                 825                 830         


Lys Tyr Gln Ser Pro Tyr Thr Gly His Ile Ile Pro Leu Ser Lys Leu 
        835                 840                 845             


Phe Thr Pro Ala Tyr Gln Ile Glu His Val Ile Pro Gln Ser Arg Tyr 
    850                 855                 860                 


Phe Asp Asp Ser Ile Ser Asn Lys Val Ile Cys Glu Ala Glu Val Asn 
865                 870                 875                 880 


Lys Glu Lys Asp Arg Met Leu Gly Tyr Glu Phe Ile Ile Asn Lys Gly 
                885                 890                 895     


Gly Ser Ile Val Lys Gly Ser Phe Gly Gln Gln Phe Lys Ile Phe Asp 
            900                 905                 910         


Lys Val Gln Tyr Glu Asp Phe Val Lys Gln Arg Tyr Ala His Asn Lys 
        915                 920                 925             


Thr Lys Met Lys Lys Leu Leu Met Asp Asp Ile Pro Asp Gly Phe Ile 
    930                 935                 940                 


Gln Arg Gln Leu Asn Asp Ser Arg Tyr Met Ser Lys Lys Ile Ile Ser 
945                 950                 955                 960 


Ile Leu Ser Asn Ile Val Arg Glu Asp Gly Glu Lys Glu Ala Thr Ser 
                965                 970                 975     


Lys His Ile Ile Val Thr Ser Gly Ala Ile Thr Asp Arg Leu Lys Lys 
            980                 985                 990         


Glu Trp Gly Ile Lys Asp Val Trp  Asn Asp Ile Ile Ala  Pro Arg Phe 
        995                 1000                 1005             


Lys Arg  Leu Asn Lys Leu Thr  Asp Ser Asn Asn Tyr  Gly Met Glu 
    1010                 1015                 1020             


Thr Glu  Lys Glu Gly Arg Gln  Tyr Phe Gln Ile Asn  Val Pro Thr 
    1025                 1030                 1035             


Glu Leu  Ser Thr Gly Leu Ile  Lys Lys Arg Ile Asp  His Arg His 
    1040                 1045                 1050             


His Ala  Met Asp Ala Ile Ile  Ile Ala Cys Thr Thr  Arg Asn His 
    1055                 1060                 1065             


Val Asn  Phe Leu Asn Asn Ser  Thr Ala Ala Ser Asp  Gln Lys Asp 
    1070                 1075                 1080             


Ile Arg  Arg Asp Leu Gln His  Thr Leu Cys Thr Lys  Thr Lys Thr 
    1085                 1090                 1095             


Asp Ser  Asn Gly Asn Tyr Val  Trp Arg Phe Asn Lys  Pro Trp Asp 
    1100                 1105                 1110             


Thr Phe  Thr Gln Asp Thr Arg  Gln Glu Leu Glu Gly  Ile Ile Val 
    1115                 1120                 1125             


Ser Phe  Lys Gln Asn Leu Arg  Ile Ile Asn Lys Met  Ser Asn Tyr 
    1130                 1135                 1140             


Tyr Gln  His Tyr Val Asp Gly  Arg Lys Thr Ile Ser  Arg Gln Asn 
    1145                 1150                 1155             


Lys Gly  Asp Gly Trp Ala Ile  Arg Lys Ser Leu His  Lys Ala Thr 
    1160                 1165                 1170             


Val Ser  Gly Lys Val Asn Leu  Lys Arg Ser Lys Thr  Val Gly Phe 
    1175                 1180                 1185             


Lys Glu  Ala Leu Asn Asp Trp  Ser Ala Ile Lys Asp  Lys Thr Ser 
    1190                 1195                 1200             


Lys Ala  Ile Leu Gln Lys Ile  Ile Ala Gln Tyr Lys  Asn Tyr Asp 
    1205                 1210                 1215             


Pro Lys  Thr Ile Phe Lys Tyr  Phe Lys Asp Arg Asn  Phe Lys Val 
    1220                 1225                 1230             


Glu Gly  Lys Asp Phe Ser Lys  Leu Glu Val Tyr Tyr  Asn Thr Ser 
    1235                 1240                 1245             


Asp Lys  Glu Pro Met Ser Ala  His Arg Val Asn Ile  Asp Thr Thr 
    1250                 1255                 1260             


Phe Asp  Glu Lys Glu Ile Arg  Ser Ile Thr Asp Thr  Gly Ile Gln 
    1265                 1270                 1275             


Lys Ile  Met Leu Lys His Leu  His Thr Tyr Asp Asp  Ala Gln Gly 
    1280                 1285                 1290             


Lys Thr  His Pro Glu Ala Ala  Phe Ser Pro Glu Gly  Ile Glu Lys 
    1295                 1300                 1305             


Met Asn  Gln Asn Ile Arg Met  Leu Asn Gly Gly Lys  Asp His Lys 
    1310                 1315                 1320             


Pro Ile  Phe Lys Val Arg Lys  Ala Glu Ile Leu Gly  Met Lys Phe 
    1325                 1330                 1335             


Ala Val  Gly Glu Arg Gly His  Lys Asp Lys Lys Trp  Val Glu Ala 
    1340                 1345                 1350             


Asp Lys  Gly Thr Asn Leu Phe  Phe Ala Ile Tyr Glu  Ser Glu Glu 
    1355                 1360                 1365             


Asn Lys  Arg Ser Tyr Ser Ser  Ile Pro Leu Asn Glu  Ala Ile Asp 
    1370                 1375                 1380             


Arg Leu  Lys Arg Gly Glu Asn  Ile Ala Asp Glu Arg  Asp Gln Asn 
    1385                 1390                 1395             


Gly Asn  Lys Leu Leu Phe Val  Leu Ser Pro Met Asp  Leu Val Tyr 
    1400                 1405                 1410             


Val Pro  Asp Pro Asp Asp Ala  His Ile Asp Glu Val  Lys Gly Asn 
    1415                 1420                 1425             


Asn Asn  Ile Phe Lys Met Val  Ser Cys Thr Gly Thr  Glu Cys Tyr 
    1430                 1435                 1440             


Phe Val  Pro Glu Thr Tyr Ala  Asn Val Ile Cys Lys  Gly Lys Glu 
    1445                 1450                 1455             


Leu Glu  Ser Lys Asn Lys Val  Glu Ser Tyr Asp Gly  Ile Asn Ile 
    1460                 1465                 1470             


Lys Lys  Ser Cys Tyr Lys Ile  Thr Val Asp Arg Leu  Gly Lys Val 
    1475                 1480                 1485             


Arg Lys  Ile Phe Lys Gly 
    1490                 


<210>  1298
<211>  1496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1298

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Val Cys Val Asp Asp Asn Gly Asn Tyr Leu Arg Asp Ile Lys 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly Asn Phe 
        35                  40                  45              


Asp Lys Gly Val Thr Glu Ser Gln Thr Ser Val Arg Thr Gly Phe Arg 
    50                  55                  60                  


Gly Val Arg Arg Ile Arg Glu Arg Ser Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Val Leu Gly Tyr Leu Pro Pro His Tyr Asp Ser 
                85                  90                  95      


Ala Ile Gly Trp Asn Lys Asn Glu Pro Ser Thr Tyr Gly Lys Phe Leu 
            100                 105                 110         


Asn His Gly Glu Pro Lys Leu Ala Trp Glu His Lys Glu Asp Gly Ser 
        115                 120                 125             


Met Arg Phe Leu Phe Met Glu Ser Phe His Glu Met Met Ala Asp Phe 
    130                 135                 140                 


Ala Lys Ser Gln Pro Ser Leu Val Ser Asp Gly Lys Lys Ile Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Pro Ile 
                165                 170                 175     


Arg Lys Glu Glu Leu Ala Trp Leu Leu Leu Asn Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Asp Asp Asp Asn Pro Thr Lys 
        195                 200                 205             


Cys Glu Glu Tyr Tyr Glu Leu Lys Val Val Gly Ile Glu Ala Asp Glu 
    210                 215                 220                 


Glu Gly Lys Asn Gly Asn Thr Trp Tyr Asn Val Lys Leu Glu Asn Gly 
225                 230                 235                 240 


Trp Ile Tyr Arg Arg Gln Ser Lys Ile Pro Leu Asp Asp Trp Ile Gly 
                245                 250                 255     


Lys Val Arg Glu Phe Ile Val Thr Thr Glu Tyr Glu Lys Asp Gly Ile 
            260                 265                 270         


Thr Leu Lys Lys Asp Lys Asn Gly Asp Val Lys Arg Ser Phe Arg Ser 
        275                 280                 285             


Pro Ser Glu Asp Asp Trp Gly Leu Arg Lys Lys Arg Thr Glu Lys Leu 
    290                 295                 300                 


Leu Asp Ser Ser Lys Met Thr Val Gly Ala Phe Val Tyr Asp His Leu 
305                 310                 315                 320 


Leu Ala Glu Pro Ser Asp Lys Ile Arg Gly Lys Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Lys Glu Leu Tyr Ala Ile Leu Cys Glu Gln 
            340                 345                 350         


Ser Lys Tyr His Glu Glu Leu Arg Ser Gly Asp Tyr Leu Asn Lys Cys 
        355                 360                 365             


Ala Glu Glu Leu Tyr Lys Asn Asn Gln Pro His Arg Asn Ser Leu Leu 
    370                 375                 380                 


Thr Lys Asp Leu Ala Tyr Leu Leu Leu Glu Asp Leu Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Thr Lys Lys Ser Leu Ile Ala Glu Cys Pro Tyr Glu 
                405                 410                 415     


Ala Tyr Lys Tyr Val Asp Lys Glu Thr Gly Glu Ile Lys Arg Gln Gly 
            420                 425                 430         


Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Ile Ser Asn Leu Arg Leu Phe Asn Lys Ala Asp Asp Lys 
    450                 455                 460                 


Glu Val Thr Ala Asp Tyr Leu Pro Thr Gln Asp Asp Tyr Val Arg Leu 
465                 470                 475                 480 


Phe Ala Tyr Leu Asn Asp Arg Lys Glu Ile Asn Gln Glu Thr Leu Leu 
                485                 490                 495     


Lys Glu Phe Phe Lys Leu Lys Lys Ile Thr Val Asn Ser Glu Lys Val 
            500                 505                 510         


Phe Pro Ile Arg Trp Asn Tyr Ile Glu Asp Lys Glu Lys Lys Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg His Glu Leu Leu Leu Ala Leu Asp Arg Ala Arg 
    530                 535                 540                 


Ile Asp His Ser Trp Leu Glu Lys Pro Gly Gln Glu Tyr Arg Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Val Glu Ser Lys Glu Glu Thr Lys Glu Ala Leu 
                565                 570                 575     


Arg Lys Leu Lys Asp Asp Asp Ala Phe Val Glu Ser Phe Leu Lys Val 
            580                 585                 590         


Lys Pro Phe Lys Arg Glu Tyr Gly Ala Tyr Ser Glu Lys Ala Ile Lys 
        595                 600                 605             


Lys Leu Leu Ala Val Met Arg Met Gly Ser Leu Trp Arg Glu Thr Asp 
    610                 615                 620                 


Ile Cys Asp Thr Thr Lys Gly Asn Ile Thr Lys Ile Leu Gln Gly Glu 
625                 630                 635                 640 


Ile Asp Glu Lys Leu Lys Glu Lys Ile Ser Ala Ser Val Ser Ser Tyr 
                645                 650                 655     


Asn Gln Leu Ser Asp Phe Gln Gly Leu Pro Val Trp Leu Ala Cys Tyr 
            660                 665                 670         


Val Val Tyr Gly Arg His Ser Glu Ala Ala Glu Ile Gln His Trp Lys 
        675                 680                 685             


Thr Pro Glu Ser Leu Leu Ala Tyr Ile Asn Gly Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Cys Ile Leu Glu Thr Leu Arg Thr 
705                 710                 715                 720 


Val His Asp Ile Trp Lys Glu Val Gly His Ile Asp Glu Ile His Val 
                725                 730                 735     


Glu Leu Gly Arg Asn Met Lys Ser Thr Ala Asp Gln Arg Ala Arg Ile 
            740                 745                 750         


Ser Glu Asn Val Leu Arg Asn Glu Asn Thr Asn Leu Arg Ile Lys Ser 
        755                 760                 765             


Leu Leu Leu Glu Leu Lys Asn Asp Pro Asn Ile Lys Asp Val Arg Pro 
    770                 775                 780                 


Tyr Ser Pro Met Gln Gln Glu Lys Leu Arg Ile Tyr Glu Glu Gly Val 
785                 790                 795                 800 


Leu Gln Glu Leu Thr Lys Asp Asp Lys Asp Phe Thr Glu Ile Thr Lys 
                805                 810                 815     


Ile Ser Thr Met Ala Gln Pro Ser Ala Lys Glu Leu Gln Arg Tyr Lys 
            820                 825                 830         


Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Ser Ile 
        835                 840                 845             


Ser Leu Thr Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Val Ile 
    850                 855                 860                 


Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val Ile Cys 
865                 870                 875                 880 


Glu Ser Glu Val Asn Ser Leu Lys Asp Asn Met Leu Gly Tyr Glu Phe 
                885                 890                 895     


Ile Lys Asn His Gly Gly Glu Ile Ile His Cys Thr Met Leu Gly Asp 
            900                 905                 910         


Val Lys Val Leu Ser Glu Ser Glu Tyr Lys Ala Phe Val Asn Asp His 
        915                 920                 925             


Tyr Ala His Asn Lys Arg Lys Arg Glu Asn Leu Leu Leu Glu Asp Ile 
    930                 935                 940                 


Pro Gln Glu Phe Leu Asn Arg Gln Met Asn Asp Ser Arg Tyr Ile Ser 
945                 950                 955                 960 


Lys Val Val Lys Ala Leu Leu Ser Asn Ile Val Arg Ala Glu Gly Glu 
                965                 970                 975     


Glu Glu Ala Thr Ser Lys Phe Val Val Ser Cys Ser Gly Gly Ile Thr 
            980                 985                 990         


Asp Arg Leu Lys Lys Asp Trp Gly  Leu Asn Asp Val Trp  Asn Lys Ile 
        995                 1000                 1005             


Val Tyr  Pro Arg Phe Glu Arg  Leu Asn Arg Leu Thr  Gly Thr Glu 
    1010                 1015                 1020             


Ala Phe  Gly Gln Trp Glu Asn  Lys Asp Gly Lys Arg  Val Phe Gln 
    1025                 1030                 1035             


Thr Ala  Val Pro Leu Glu Leu  Gln Arg Gly Phe Asn  Lys Lys Arg 
    1040                 1045                 1050             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1055                 1060                 1065             


Thr Ser  Arg Asn Ile Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Asn 
    1070                 1075                 1080             


Asn Pro  Lys Lys Arg Glu Asp  Leu Arg Gln Lys Leu  Cys Asp Lys 
    1085                 1090                 1095             


Asn Arg  Thr Ile Arg Lys Pro  Trp Glu Thr Phe Thr  Gln Asp Ala 
    1100                 1105                 1110             


Thr Ala  Ala Leu Glu Asp Val  Ile Val Ser Phe Lys  Asn Tyr Val 
    1115                 1120                 1125             


Arg Ile  Ile Asn Lys Ala Thr  Asn Tyr Tyr Glu Arg  Tyr Asp Val 
    1130                 1135                 1140             


Glu Gly  Lys Lys Val Ile Ala  Glu Gln Arg Gly Asp  Asp Met Trp 
    1145                 1150                 1155             


Ala Ile  Arg Lys Pro Met His  Glu Ala Thr Tyr Tyr  Gly Arg Val 
    1160                 1165                 1170             


Asn Leu  Arg Arg Lys Lys Lys  Val Asn Leu Arg Val  Ala Leu Glu 
    1175                 1180                 1185             


Asn Ile  Ser Ser Ile Cys Asp  Asn Lys Leu Arg Ile  Tyr Ile Asn 
    1190                 1195                 1200             


Gly Leu  Val Glu Lys Gly Phe  Asn Thr Lys Gln Ile  Leu Asp Lys 
    1205                 1210                 1215             


Phe Lys  Asn Thr Asn Asp His  Phe Ser Met Gln Lys  Ile Asp Lys 
    1220                 1225                 1230             


Val Glu  Ile Trp Gln Phe Thr  Asn Glu Glu Glu Lys  Leu Val Ala 
    1235                 1240                 1245             


Thr Arg  Lys Pro Leu Asp Thr  Ser Phe Asp Thr Lys  Lys Ile Ala 
    1250                 1255                 1260             


Ser Ile  Thr Asp Thr Gly Ile  Gln Thr Ile Leu Leu  Asn Tyr Leu 
    1265                 1270                 1275             


Glu Thr  Lys Gly Gly Asp Ser  Thr Ile Ala Phe Thr  Pro Glu Gly 
    1280                 1285                 1290             


Ile Val  Glu Met Asn Gln Asn  Ile Ser Lys Tyr Asn  Asn Gly Lys 
    1295                 1300                 1305             


Glu His  Met Pro Ile Leu Lys  Val Arg Leu Tyr Thr  Lys Leu Gly 
    1310                 1315                 1320             


Lys Lys  Ile Pro Val Gly Gln  Thr Gly Cys Lys Ala  Lys Gln Phe 
    1325                 1330                 1335             


Val Ile  Ala Glu Ala Gly Thr  Asn Leu Tyr Phe Ala  Ile Tyr Glu 
    1340                 1345                 1350             


Asp Glu  Glu Gly Lys Arg Ser  Tyr Arg Thr Ile Pro  Leu Asn Glu 
    1355                 1360                 1365             


Val Val  Glu Arg Leu Lys Gln  Gly Leu Ser Pro Val  Pro Glu Lys 
    1370                 1375                 1380             


Asn Asp  Lys Asp Val His Leu  Lys Phe Tyr Leu Ser  Pro Asn Asp 
    1385                 1390                 1395             


Leu Val  Tyr Val Pro Ile Glu  Asp Glu His Glu Ser  Leu Ala Lys 
    1400                 1405                 1410             


Asp His  Ile Tyr Lys Phe Val  Asp Ser Ser Gly Thr  Thr Ala Asn 
    1415                 1420                 1425             


Phe Ile  Pro His Arg Ser Ala  Asn Val Ile Phe Glu  Leu Asn Lys 
    1430                 1435                 1440             


Lys Asp  Ala Glu Lys Phe Cys  Ser Gly Ser Ile Ile  Gln Asn Glu 
    1445                 1450                 1455             


Tyr Gly  Val Gly Ser Ser Gln  Ser Lys Asn Gln Lys  Ala Ile Thr 
    1460                 1465                 1470             


Gly Glu  Met Ile Lys Ala Val  Cys Arg Lys Leu Glu  Val Asp Arg 
    1475                 1480                 1485             


Leu Gly  Asn Val Ile Lys Val  Ile 
    1490                 1495     


<210>  1299
<211>  1498
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1299

Met Lys His Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Ile Arg Ala His Gln Asp Ala Asp Gly His Thr Thr Leu Gln 
            20                  25                  30          


Asp Ile Leu Ala Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Thr 
        35                  40                  45              


Leu Thr Asp Phe Asp Lys Gly Asn Ala Arg Ser Gly Thr Ala Glu Arg 
    50                  55                  60                  


Thr Ser Phe Arg Ser Met Arg Arg Arg Arg Glu Arg Gln Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Val Leu Met Leu Leu Gly Phe Leu Pro Gln 
                85                  90                  95      


His Tyr Ala Asn Ser Leu Thr Arg Tyr Gly Glu Phe Ala Asp Asn Arg 
            100                 105                 110         


Glu Cys Lys Leu Pro Trp Val Lys Asp Glu Ser Asp Gly Lys Tyr His 
        115                 120                 125             


Phe Leu Phe Gln Asp Ala Phe His Glu Met Cys Glu Asp Phe Arg Gln 
    130                 135                 140                 


Leu Ala Pro Gln Trp Val Ala Asp Gly Lys Lys Val Pro Tyr Asp Trp 
145                 150                 155                 160 


Thr Leu Tyr Tyr Leu Arg Cys Lys Ala Leu Ser Arg Lys Ile Ser Lys 
                165                 170                 175     


Glu Glu Leu Ala Trp Ile Leu Leu Ser Phe Asn Gln Lys Arg Gly Tyr 
            180                 185                 190         


Tyr Gln Ala Arg Asp Glu Glu Asp Glu Val Thr Thr Asn Lys Leu Glu 
        195                 200                 205             


Glu Tyr Tyr Ala Gly Lys Val Val Arg Val Asp Asp Thr Gly Glu Arg 
    210                 215                 220                 


Lys Gly Lys Asn Val Tyr Tyr Asp Ile Thr Leu Glu Asn Gly Trp Val 
225                 230                 235                 240 


Tyr Arg Tyr Ala Ala Arg Ile Tyr Pro Asp Trp Val Gly Gln Val Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Thr Leu Asn Pro Asp Gly Thr Pro Lys Thr 
            260                 265                 270         


Asp Lys Ala Gly Glu Val Lys Arg Ser Phe Arg Met Pro Lys Asp Asp 
        275                 280                 285             


Asp Trp Thr Leu Ile Lys Lys Lys Lys Glu Arg Asp Ile Asp Arg Ser 
    290                 295                 300                 


Gln Glu Thr Ile Gly Gln Tyr Ile Tyr His Ala Leu Leu Lys Asp Pro 
305                 310                 315                 320 


Ser Gln Lys Ile Arg Gly Lys Leu Ile Gly Thr Val Glu Arg Lys Tyr 
                325                 330                 335     


Tyr Lys Asp Glu Leu Arg Arg Ile Leu Asp Arg Gln Ile Gln Phe His 
            340                 345                 350         


Pro Glu Leu Arg Asp Arg Glu Leu Tyr Tyr Ala Cys Leu Gln Glu Leu 
        355                 360                 365             


Tyr Pro Ser Asn Val Ala Phe Arg Asn Thr Leu Val Asn Arg Asp Phe 
    370                 375                 380                 


Thr Tyr Leu Phe Leu Asp Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys 
385                 390                 395                 400 


Ser Lys Lys Ser Gln Ile Ser Asp Cys Pro Tyr Glu Glu His Phe Tyr 
                405                 410                 415     


Thr Asp Lys Glu Thr Gly Glu Arg Ile Ser Val Pro Val Lys Cys Ile 
            420                 425                 430         


Ala Val Ser His Pro Leu Tyr Gln Glu Tyr Arg Leu Trp Gln Phe Ile 
        435                 440                 445             


Gln Asn Leu Arg Ile Tyr Cys Arg Glu Ala Arg Ile Asp Gly Asn Leu 
    450                 455                 460                 


Met Ile Asp Trp Glu Val Thr Asn Glu Leu Leu Gln Thr Glu Glu Asp 
465                 470                 475                 480 


Lys Thr Ala Leu Phe Asp Trp Leu Asn Asp Lys Glu Ser Val Lys Gln 
                485                 490                 495     


Pro Glu Leu Val Ala Phe Leu Leu Gly Gly Lys Ala Asn Lys Lys Gln 
            500                 505                 510         


Ile Lys Ala Tyr Arg Trp Asn Tyr Met Asp Asp Asp Thr Lys Gly Tyr 
        515                 520                 525             


Pro Cys Asn Glu Thr Arg Ala Ser Leu Leu Lys Tyr Leu Arg Glu Ala 
    530                 535                 540                 


Gln Thr Asp Ser Ser Phe Leu Thr Arg Glu Arg Glu Glu Ala Leu Trp 
545                 550                 555                 560 


His Ile Leu Tyr Ser Ile Ser Asp Lys Gly Glu Leu Lys Ser Ala Leu 
                565                 570                 575     


Gly Lys Phe Ala Val Lys His Gly Leu Asn Glu Arg Phe Val Thr Val 
            580                 585                 590         


Phe Ser Lys Phe Pro Pro Phe Lys Lys Asp Tyr Gly Ser Tyr Ser Ala 
        595                 600                 605             


Lys Ala Leu Lys Lys Leu Leu Pro Leu Met Arg Met Gly His His Trp 
    610                 615                 620                 


Gln Glu Ala Ser Phe Ile Pro Ser Thr Arg Gln Arg Met Glu Lys Phe 
625                 630                 635                 640 


Ile Asn Gly Glu Tyr Asp Asp Ser Ile Asp Lys Arg Thr Tyr Glu Lys 
                645                 650                 655     


Leu Glu Ser Leu Thr Glu Val Ser Gln Tyr Arg Gly Leu Pro Gly Trp 
            660                 665                 670         


Leu Ala Cys Tyr Val Val Tyr Asp Arg His Ser Glu Gly Arg Asp Ile 
        675                 680                 685             


Glu Arg Trp Thr Ser Pro Ser Asp Ile Asp Ser Tyr Leu Ala Gln Phe 
    690                 695                 700                 


Lys Gln His Ser Leu His Asn Pro Ile Val Glu Arg Val Val Leu Glu 
705                 710                 715                 720 


Thr Leu Arg Thr Val Arg Asp Ile Trp Lys Gln Val Asp His Ile Asp 
                725                 730                 735     


Glu Ile His Ile Glu Leu Gly Arg Asn Met Lys Leu Pro Ala Asp Lys 
            740                 745                 750         


Arg Arg Gln Met Thr Glu Gln Ala Trp Lys Asn Glu Gln Thr Asn Leu 
        755                 760                 765             


Arg Ile Lys Ala Leu Leu Leu Glu Phe Ala Arg Pro Gly Tyr Asp Ile 
    770                 775                 780                 


Glu Asn Val Arg Pro Tyr Ser Pro Tyr Gln Gln Asp Gln Leu Arg Ile 
785                 790                 795                 800 


Tyr Glu Glu Ala Val Leu Gly Ala Asn Glu Cys Pro Asp Asp Ile Ser 
                805                 810                 815     


Ala Ile Leu Lys Lys Phe Asn Gly Leu Asn Pro Pro Thr Ser Ala Glu 
            820                 825                 830         


Val Leu Arg Tyr Lys Cys Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr 
        835                 840                 845             


Thr Gly Arg Thr Ile Pro Leu Ser Lys Leu Phe Thr Ser Ala Tyr Glu 
    850                 855                 860                 


Ile Glu His Ile Ile Pro Lys Gln Arg Tyr Phe Asp Asp Ser Phe Ser 
865                 870                 875                 880 


Asn Lys Val Ile Cys Glu Ser Glu Val Asn Lys Lys Lys Gly Ala Met 
                885                 890                 895     


Leu Gly Tyr Glu Phe Ile Lys Ser Tyr His Gly Ala Ser Ile Thr Leu 
            900                 905                 910         


Asn Arg Gly Gly Thr Ala Ser Ile Phe Ser Val Glu Ala Tyr Glu Asn 
        915                 920                 925             


Phe Val Lys Glu His Tyr Ala His Asn Arg Gly Lys Met Lys Lys Leu 
    930                 935                 940                 


Leu Met Asp Asp Val Pro Asp Ser Phe Ile Ser Arg Gln Leu Asn Asp 
945                 950                 955                 960 


Ser Arg Tyr Ile Ser Lys Leu Met Lys Ser Ile Leu Ser Asn Leu Val 
                965                 970                 975     


Arg Glu Glu Gly Glu Lys Glu Ala Ile Ser Lys Asn Leu Ile Val Cys 
            980                 985                 990         


Thr Gly Gly Ile Thr Asp Arg Leu  Lys Lys Asp Trp Gly  Ile His Asp 
        995                 1000                 1005             


Val Trp  Asn Asp Ile Ile Phe  Pro Arg Phe Lys Arg  Leu Asp Glu 
    1010                 1015                 1020             


Met Asp  Ser Ser His Thr Tyr  Ile Thr Leu Asn Thr  His Arg His 
    1025                 1030                 1035             


Pro Ile  Pro Ser Met Pro Leu  Glu Phe Gln Arg Gly  Phe Ser Lys 
    1040                 1045                 1050             


Lys Arg  Ile Asp His Arg His  His Ala Met Asp Ala  Ile Val Ile 
    1055                 1060                 1065             


Ala Cys  Ala Thr Arg Asn Ile  Val Asn Tyr Leu Asn  Asn Glu Ser 
    1070                 1075                 1080             


Ala Arg  His Asp Ala Lys Thr  Ser Arg Met Asp Leu  Gln Arg Leu 
    1085                 1090                 1095             


Leu Cys  Thr Ala Asn Arg Lys  Leu Ile Asp Lys Pro  Trp Lys Thr 
    1100                 1105                 1110             


Phe Thr  Gln Asp Thr Cys Lys  Val Leu Glu Asp Met  Ile Val Ser 
    1115                 1120                 1125             


Phe Gln  Arg Thr Leu Arg Ile  Ile Asn Lys Thr Lys  Asn Tyr Tyr 
    1130                 1135                 1140             


Leu Arg  Phe Glu Asp Gly Lys  Lys Arg Arg Val Leu  Gln Thr Lys 
    1145                 1150                 1155             


Gly Asp  Asn Trp Ala Ile Arg  Lys Pro Leu His Lys  Glu Thr Phe 
    1160                 1165                 1170             


Tyr Gly  Glu Ile Asn Leu Arg  Arg Ile Glu Thr Leu  Ser Leu Asn 
    1175                 1180                 1185             


Lys Ala  Leu Glu Arg Ile Pro  Asp Ile Val Glu Arg  Asp Leu Arg 
    1190                 1195                 1200             


Lys Lys  Leu Ser Glu Leu Leu  Ser Leu Gly Tyr Asn  Ala Lys Arg 
    1205                 1210                 1215             


Ile Lys  Gly Tyr Phe Glu Glu  His Lys Glu Glu Trp  Ser Asp Val 
    1220                 1225                 1230             


Asn Leu  Ser Lys Ile Lys Ala  Tyr Tyr Phe Thr Lys  Gln Lys Asn 
    1235                 1240                 1245             


Lys Arg  Tyr Phe Ala Ala Arg  Thr Glu Leu Asp Thr  Ser Phe Asn 
    1250                 1255                 1260             


Lys Asp  Lys Ile Glu Asn Lys  Ile Ala Asp Thr Ala  Ile Gln Lys 
    1265                 1270                 1275             


Ile Leu  Leu Gln His Leu Glu  Ala Tyr Gly Asn Asp  Val Glu Gln 
    1280                 1285                 1290             


Ala Phe  Ser Pro Glu Gly Ile  Asp Thr Met Asn Arg  Asn Ile Arg 
    1295                 1300                 1305             


Gln Leu  Asn Gly Gly Arg Ser  His Gln Pro Ile Tyr  Lys Val Arg 
    1310                 1315                 1320             


Val Tyr  Glu Glu Ala Thr Lys  Phe Ala Val Gly Arg  Arg Gly Asn 
    1325                 1330                 1335             


Lys Ser  Thr Lys Phe Val Glu  Ala Ala Lys Gly Thr  Asn Leu Phe 
    1340                 1345                 1350             


Phe Ala  Ile Tyr Glu Ile Ser  Gln Val Asp Gly Asn  Gly Asn Arg 
    1355                 1360                 1365             


Gln Thr  Ser Arg Thr Phe Ser  Ser Ile Pro Leu Asn  Val Ala Val 
    1370                 1375                 1380             


Glu Arg  Leu Lys Gln Gly Leu  Pro Pro Val Pro Glu  Asp Glu Glu 
    1385                 1390                 1395             


Gly Asn  Ala Pro Lys Tyr Val  Leu Ser Pro Asn Asp  Leu Val Tyr 
    1400                 1405                 1410             


Leu Pro  Thr Glu Lys Glu Leu  Lys Arg Arg Glu Ile  Ala Met Pro 
    1415                 1420                 1425             


Ile Asp  Lys Ser Arg Ile Tyr  Lys Met Val Ser Cys  Thr Gly Lys 
    1430                 1435                 1440             


Asp Cys  Phe Phe Ile Pro Ala  Asn Thr Ala Val Pro  Ile Val Asp 
    1445                 1450                 1455             


Arg Tyr  Glu Tyr Ser Ser Met  Asn Lys Ile Gly Arg  Ala Leu Thr 
    1460                 1465                 1470             


Gly Glu  Met Ile Lys Glu Ile  Cys Leu Pro Leu Lys  Ile Asp Arg 
    1475                 1480                 1485             


Leu Gly  Gln Ile Ile Glu Ile  Ser Thr Phe 
    1490                 1495             


<210>  1300
<211>  1501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1300

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Lys Ala Glu Asn Glu Gly Lys Asp Val Leu Cys Gly 
            20                  25                  30          


Ile Glu Ala Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Thr Ile Met 
        35                  40                  45              


Gly Asp Phe Glu Lys Gly Asn Ser Val Ser Gln Thr Lys Asp Arg Arg 
    50                  55                  60                  


Glu His Arg Ser Thr Arg Arg Leu Tyr Glu Arg Gly Lys Leu Arg Arg 
65                  70                  75                  80  


Glu Arg Leu Asn Arg Val Leu Ser Val Met Gly Phe Leu Pro Asp His 
                85                  90                  95      


Tyr Val Ser Gln Leu Asp Arg Tyr Gly Lys Phe Leu Ser Asn Thr Glu 
            100                 105                 110         


Pro Lys Leu Ala Trp Val Arg Asn Ala Asp Cys Lys Tyr His Phe Leu 
        115                 120                 125             


Phe Met Asp Ser Phe Ser Glu Met Leu Gln Glu Phe Lys Thr Asn Ser 
    130                 135                 140                 


Pro Gln Tyr Ile Ser Glu Glu Ser Lys Val Pro Tyr Asp Trp Thr Leu 
145                 150                 155                 160 


Tyr Tyr Leu Arg Lys Lys Ala Leu Thr His Lys Val Thr Lys Glu Glu 
                165                 170                 175     


Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Phe Gln 
            180                 185                 190         


Leu Arg Asp Glu Ser Glu Glu Glu Lys Lys Pro Ser Lys Ile Arg Gln 
        195                 200                 205             


Tyr Phe Glu His Gln Lys Val Val Asp Ile Ile Asp Thr Asn Glu Val 
    210                 215                 220                 


Tyr Lys Gly Leu Lys Val Leu Ile Val Ile Leu Ala Asn Gly Ile Ser 
225                 230                 235                 240 


Gly Lys Ile Phe Lys Lys Glu Ile Pro Asp Trp Ile Gly Val Glu Lys 
                245                 250                 255     


Asp Ile Ile Val Lys Val Asp Ile Asp Lys Asp Gly Lys Asp Lys Phe 
            260                 265                 270         


Asn Glu Asp Gly Ser Leu Ser Cys Arg Phe Asn Ile Pro Thr Glu Ala 
        275                 280                 285             


Glu Trp Asp Thr Glu Trp Glu Leu Ile Lys Gln Lys Thr Gln Asn Asp 
    290                 295                 300                 


Leu Asp Ala Ser Gly Lys Thr Val Gly Glu Tyr Ile Phe Asp Thr Leu 
305                 310                 315                 320 


Leu Arg Asn Pro Glu Gln Lys Ile Arg Gly Lys Leu Val Arg Thr Ile 
                325                 330                 335     


Glu Arg Arg Tyr Tyr Lys Glu Glu Ile Asn Ala Ile Leu Lys Lys Gln 
            340                 345                 350         


Lys Glu Phe His Pro Glu Leu Ser Asp Arg Ala Leu Tyr Glu Lys Cys 
        355                 360                 365             


Val Glu Glu Leu Tyr Pro Asn Asn Pro Ala His Arg Asn Met Met Leu 
    370                 375                 380                 


Ile His Asp Leu Ser Tyr Phe Leu Val Asn Asp Ile Leu Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Asp Glu Cys Pro Tyr Glu 
                405                 410                 415     


Ser His Leu Tyr Ile Asp Lys Lys Ser Gly Glu Arg Lys Pro Val Ala 
            420                 425                 430         


Val Lys Cys Ile Ala Lys Ser His Pro Leu Tyr Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Ile Ser Asn Leu Arg Ile Tyr Gln Lys Thr Lys Glu Val 
    450                 455                 460                 


Gly Asn Ser Leu Lys Val Asp Val Asp Val Thr Asn Glu Phe Leu Lys 
465                 470                 475                 480 


Gly Glu Asn Asp Tyr Val Glu Leu Phe Asp Trp Leu Asn Thr Lys Lys 
                485                 490                 495     


Glu Ile Lys Gln Glu Asp Phe Leu Lys Tyr Lys Pro Phe Gly Phe Thr 
            500                 505                 510         


Lys Gly Asn Ile Asn Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys Ile 
        515                 520                 525             


Tyr Pro Cys Asn Lys Thr His Tyr Thr Ile Leu Lys Gly Leu Glu Lys 
    530                 535                 540                 


Ala Asp Ile Thr Phe Asp Glu Phe Ile Ser His Asp Ser Leu Ala Glu 
545                 550                 555                 560 


Glu Asn Leu Trp His Ile Leu Tyr Ser Val Ser Ser Lys Glu Glu Leu 
                565                 570                 575     


Lys His Ala Leu Glu Ser Phe Ala Asp Lys Arg Gly Leu Ser Ile Lys 
            580                 585                 590         


Phe Val Asp Ala Phe Ile Asn Thr Pro Ala Phe Glu Lys Ser Tyr Gly 
        595                 600                 605             


Ala Tyr Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Met 
    610                 615                 620                 


Gly Lys Tyr Trp Lys Glu Glu Asn Ile Cys Asp Ser Thr Arg Glu Arg 
625                 630                 635                 640 


Ile Asp Lys Ile Ile Thr Gly Glu Tyr Asp Glu Asn Ile Arg Asp Arg 
                645                 650                 655     


Val Arg Glu Lys Ser Ile Asn Leu Thr Asp Ile Thr Leu Phe Arg Gly 
            660                 665                 670         


Leu Pro Leu Trp Leu Ala Cys Tyr Val Val Tyr Asp Arg His Ser Glu 
        675                 680                 685             


Ala Gly Asn Thr Glu Arg Trp Thr Ser Pro Ser Asp Ile Asp His Tyr 
    690                 695                 700                 


Ile Arg Lys Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln 
705                 710                 715                 720 


Ile Val Leu Glu Thr Leu Arg Thr Val Arg Asp Val Trp Thr Glu Val 
                725                 730                 735     


Gly Gly Ile Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys Asn 
            740                 745                 750         


Pro Ser Asp Lys Arg Arg Lys Met Ser Leu Gln Met Gln Glu Asn Glu 
        755                 760                 765             


Asn Thr Asn Leu Arg Ile Lys Ala Leu Leu Ala Glu Phe Ile Asn Pro 
    770                 775                 780                 


Glu Leu Gly Ile Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln Asp 
785                 790                 795                 800 


Leu Leu Arg Leu Tyr Glu Glu Gly Ala Trp Asn Ser Pro Glu Ile Asp 
                805                 810                 815     


Glu Lys Gln Val Asp Glu Met Leu Ala Ile Leu Lys Lys Phe Asn Glu 
            820                 825                 830         


Thr Asp Ser Ser Lys Arg Pro Thr Lys Ser Glu Ile Lys Arg Tyr Arg 
        835                 840                 845             


Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Thr Pro Ile 
    850                 855                 860                 


Pro Leu Ser Lys Leu Phe Thr Thr Ala Tyr Glu Ile Glu His Val Ile 
865                 870                 875                 880 


Pro Arg Ser Arg Phe Phe Asp Asp Ser Leu Thr Asn Lys Val Ile Cys 
                885                 890                 895     


Glu Ser Glu Val Asn Lys Leu Lys Gly Asn Met Leu Gly Tyr Glu Phe 
            900                 905                 910         


Ile Lys Ser His Tyr Gly Glu Lys Val Gln Leu Ser Ser Gly Ser Glu 
        915                 920                 925             


Val Gln Ile Phe Ser Val Asn Asp Tyr Glu Lys Phe Val Lys Asp His 
    930                 935                 940                 


Tyr Ser Lys Asn Lys Ala Lys Met Lys Lys Leu Leu Met Asp Asp Ile 
945                 950                 955                 960 


Pro Glu Lys Phe Thr Glu Arg Gln Met Asn Asp Ser Arg Tyr Ile Ser 
                965                 970                 975     


Arg Leu Ile Val Ser Leu Leu Ser Asn Ile Val Arg Glu Lys Gly Glu 
            980                 985                 990         


Gln Glu Ser Ile Ser Lys Asn Val  Leu Val Cys Thr Gly  Gly Val Thr 
        995                 1000                 1005             


Asp Arg  Leu Lys Asn Asp Trp  Gly Val Asn Asp Val  Trp Asn Lys 
    1010                 1015                 1020             


Ile Ile  Leu Pro Arg Phe Lys  Arg Met Asn Glu Ile  Thr Asp Ser 
    1025                 1030                 1035             


Thr Asp  Tyr Thr Ser Val Asn  Thr Ser Gly His Ile  Ile Pro Ala 
    1040                 1045                 1050             


Met Pro  Leu His Leu Gln Lys  Gly Phe Asn Lys Lys  Arg Ile Asp 
    1055                 1060                 1065             


His Arg  His His Ala Met Asp  Ala Ile Val Ile Ala  Cys Ala Gly 
    1070                 1075                 1080             


Arg Asn  Ile Val Asn Tyr Leu  Asn Asn Gln Ser Ser  Lys Ser Asp 
    1085                 1090                 1095             


Val Ser  Arg His Asp Leu Arg  Asn Leu Leu Cys Glu  Lys Asp Lys 
    1100                 1105                 1110             


Ser Asp  Arg Lys Glu Leu Ile  Ile Lys Pro Trp Asp  Thr Phe Thr 
    1115                 1120                 1125             


Gln Asp  Val Tyr Ser Val Leu  Ser Ser Ile Ile Val  Ser Ile Lys 
    1130                 1135                 1140             


Gln Asn  Leu Arg Val Ile Asn  Lys Thr Lys Asn Ile  Tyr Gln Arg 
    1145                 1150                 1155             


Lys Glu  Asp Gly Lys Val Ile  Ile Ser Lys Gln Glu  Asn Asn Ile 
    1160                 1165                 1170             


Asn Trp  Trp Ala Ile Arg Lys  Pro Leu His Lys Glu  Thr Val Ser 
    1175                 1180                 1185             


Gly Glu  Val Asn Leu Arg Lys  Glu Thr Thr Ile Ser  Leu Lys Glu 
    1190                 1195                 1200             


Ala Ile  Asp Asn Pro Ala Arg  Ile Ile Asp Lys Asp  Phe Lys Lys 
    1205                 1210                 1215             


Lys Leu  Arg Glu Leu Ile Leu  Met Gly Tyr Asn Glu  Lys Leu Ile 
    1220                 1225                 1230             


Lys Lys  Tyr Phe Tyr Asp Asn  Asn Glu Ser Trp Gln  Asp Val Asn 
    1235                 1240                 1245             


Leu Lys  Lys Ile Pro Ala Tyr  Tyr Phe Thr Lys Asp  Thr Asn Asp 
    1250                 1255                 1260             


Arg Tyr  Phe Ala Ser Arg Ser  Leu Leu Asp Glu Asn  Phe Asp Lys 
    1265                 1270                 1275             


Lys Lys  Ile Glu Asn Lys Val  Thr Asp Thr Gly Ile  Arg Thr Ile 
    1280                 1285                 1290             


Leu Leu  Arg His Leu Glu Val  Glu Gly Gly Asp Ser  Lys Val Ala 
    1295                 1300                 1305             


Phe Ser  Pro Glu Gly Leu Glu  Arg Met Asn Lys Asn  Ile Glu Ile 
    1310                 1315                 1320             


Leu Asn  Gly Gly Lys Lys His  Lys Pro Ile Tyr Lys  Val Arg Glu 
    1325                 1330                 1335             


Tyr Glu  Lys Ala Glu Lys Phe  Ala Val Gly Met Arg  Gly Asn Lys 
    1340                 1345                 1350             


Ala Ser  Lys Phe Val Glu Ala  Ala Lys Gly Thr Asn  Leu Phe Phe 
    1355                 1360                 1365             


Ala Val  Tyr Asp Asn Gly Lys  Lys Arg Ser Phe Ala  Thr Val Pro 
    1370                 1375                 1380             


Leu Asn  Glu Val Ile Arg Leu  Gln Lys Glu Gly Leu  Pro Ser Ala 
    1385                 1390                 1395             


Pro Ala  Asn Glu Glu Gly Leu  Leu Pro Lys Phe Val  Leu Ser Pro 
    1400                 1405                 1410             


Asn Asp  Leu Val Tyr Val Pro  Thr Glu Asp Glu Leu  Arg Ser Gly 
    1415                 1420                 1425             


Leu Leu  Pro Glu Asn Pro Ala  Pro Glu Arg Ile Tyr  Lys Met Val 
    1430                 1435                 1440             


Ser Cys  Thr Gly Tyr Gln Cys  Phe Phe Ile His Phe  Ser Val Ser 
    1445                 1450                 1455             


Gly Val  Leu Cys Asp Lys Lys  Glu Phe Ser Ser Leu  Asn Lys Met 
    1460                 1465                 1470             


Glu Arg  Ala Ile Ser Gly Glu  Met Ile Lys Glu Ile  Cys Ile Pro 
    1475                 1480                 1485             


Leu Lys  Val Asn Arg Leu Gly  Lys Ile Met Tyr Asn  Leu 
    1490                 1495                 1500     


<210>  1301
<211>  1501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1301

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Ile Ser Ile Asp Asp Asn Gly Asn Tyr Leu Arg Asp Ile Gln 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly Lys Phe 
        35                  40                  45              


Asp Asn Gly Val Thr Glu Ser Gln Thr Ala Val Arg Thr Gly Phe Arg 
    50                  55                  60                  


Gly Leu Arg Arg Ile Arg Glu Arg Ser Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Val Met Gly Phe Leu Pro Pro His Tyr Asp Ser 
                85                  90                  95      


Ala Ile Gly Trp Asn Lys Glu Glu Pro Ser Thr Tyr Gly Lys Phe Leu 
            100                 105                 110         


Asn His Gly Glu Pro Lys Leu Ala Trp Glu Arg Gln Gly Asp Gly Ser 
        115                 120                 125             


Met Arg Phe Leu Phe Met Asp Ser Phe His Lys Met Met Glu Asp Phe 
    130                 135                 140                 


Ala Pro His Gln Pro Ala Leu Val Ala Asp Gly Arg Lys Ile Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Pro Ile 
                165                 170                 175     


Lys Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu Asn Pro Thr Lys 
        195                 200                 205             


Arg Glu Glu Tyr His Glu Leu Lys Val Val Ser Val Glu Ala Ala Glu 
    210                 215                 220                 


Glu Gly Lys Gly Gly Asn Thr Trp Tyr Asn Val Lys Leu Glu Asn Gly 
225                 230                 235                 240 


Trp Ile Tyr His Arg Pro Ser Lys Ile Asp Leu Ser Asp Trp Val Gly 
                245                 250                 255     


Lys Thr Lys Gln Phe Ile Val Thr Met Glu Tyr Glu Ala Asp Gly Ile 
            260                 265                 270         


Thr Leu Lys Lys Asn Lys Glu Gly Glu Val Lys Arg Ser Phe Arg Val 
        275                 280                 285             


Pro Asp Glu Asp Asp Trp Gly Leu Arg Lys Lys Arg Thr Glu Ser Gln 
    290                 295                 300                 


Leu Glu Ala Ser Gly Lys Thr Val Gly Ala Phe Ile Tyr Asp His Leu 
305                 310                 315                 320 


Leu Ala Glu Pro Ala Asp Lys Ile Arg Gly Asn Phe Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Gln Glu Leu Ala Asp Ile Leu Arg Glu Gln 
            340                 345                 350         


Ser Lys Tyr His Glu Glu Leu Gln Ser Lys Lys Ile Leu Ala Asp Cys 
        355                 360                 365             


Ala Glu Glu Leu Tyr Arg Ser Asn Gln Pro His Arg Asp Ser Leu Leu 
    370                 375                 380                 


Lys Lys Asp Met Val Tyr Leu Leu Leu Thr Asp Leu Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asn Cys Pro Tyr Glu 
                405                 410                 415     


Tyr Tyr Glu Tyr Val Asp Lys Glu Thr Gly Glu Ile Lys Arg Gln Gly 
            420                 425                 430         


Ile Lys Cys Val Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Val Leu Asn Leu Arg Leu Tyr Asp Arg Thr Glu Asp Lys 
    450                 455                 460                 


Glu Val Thr Ala Glu Tyr Leu Pro Ser Lys Glu Asp Tyr Ala Arg Leu 
465                 470                 475                 480 


Phe Val Tyr Leu Asn Asp Arg Lys Glu Ile Asn Gln Glu Thr Leu Leu 
                485                 490                 495     


Lys Asp Phe Phe Lys Leu Lys Lys Val Lys Gln Arg Ser Glu Lys Glu 
            500                 505                 510         


Phe Pro Val Arg Trp Asn Tyr Ile Glu Asp Lys Glu Lys Lys Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg His Glu Leu Leu Met Ala Leu Asp Arg Ala Gly 
    530                 535                 540                 


Met Glu Arg Ser Trp Leu Asp Asp Thr Ala Arg Gln Tyr Arg Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Ile Glu Val Lys Asp Glu Ala Glu Arg Ala Leu 
                565                 570                 575     


Arg Lys Leu Lys Asp Asp Asp Glu Phe Val Ser Ala Phe Leu Lys Val 
            580                 585                 590         


Lys Pro Phe Lys Lys Asp Tyr Gly Ala Tyr Ser Glu Lys Ala Val Lys 
        595                 600                 605             


Lys Leu Leu Ala Val Met Arg Met Gly Ser His Trp Cys Glu Asp Asp 
    610                 615                 620                 


Ile Cys Lys Glu Thr Lys Glu Arg Ile Glu His Ile Leu Gln Gly Asp 
625                 630                 635                 640 


Val Glu Glu Lys Ile Lys Glu Arg Met Asn His Thr Ala Tyr Ser Leu 
                645                 650                 655     


Gly Glu Leu Ser Asp Phe Gln Gly Leu Pro Glu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Val Val Tyr Gly Arg His Ser Glu Ala Thr Asp Ile Lys His Trp Lys 
        675                 680                 685             


Thr Pro Glu Ser Leu Met Ala Tyr Ile Asn Gly Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Cys Ile Leu Glu Thr Leu Arg Thr 
705                 710                 715                 720 


Val His Asp Ile Trp Lys Glu Ala Gly His Ile Asp Glu Ile His Val 
                725                 730                 735     


Glu Leu Gly Arg Ser Met Lys Ser Thr Ser Asp Gln Arg Ala Arg Met 
            740                 745                 750         


Thr Glu Asn Ile Leu Arg Asn Glu Asn Thr Asn Leu Arg Ile Lys Ser 
        755                 760                 765             


Leu Leu Met Glu Leu Lys Asn Asp Ala Asp Ile Lys Asp Val Arg Pro 
    770                 775                 780                 


Tyr Ser Pro Met Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly Ala 
785                 790                 795                 800 


Leu Gln Glu Leu Thr Lys Glu Asp Asn Asp Tyr Glu Glu Ile Phe Lys 
                805                 810                 815     


Ile Ser Lys Ile Pro Gln Pro Ser Ala Lys Glu Leu Leu Lys Tyr Lys 
            820                 825                 830         


Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Pro Ile 
        835                 840                 845             


Ser Leu Ser Lys Leu Phe Thr Ser Ala Tyr Gln Ile Glu His Ile Ile 
    850                 855                 860                 


Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Asn Asn Lys Val Ile Cys 
865                 870                 875                 880 


Glu Ser Glu Val Asn Ala Leu Lys Ser Asn Met Leu Gly Tyr Glu Phe 
                885                 890                 895     


Ile Lys Asn His Gly Gly Glu Ile Val His Cys Thr Leu Leu Gly Asp 
            900                 905                 910         


Val Lys Val Gln Asn Glu Ser Glu Tyr Lys Ala Phe Val Thr Glu His 
        915                 920                 925             


Tyr Ala Asn Asn Lys Tyr Lys Lys Glu Lys Leu Leu Met Glu Asp Ile 
    930                 935                 940                 


Pro Gln Asp Phe Leu Asn Arg His Leu Asn Asp Ser Arg Tyr Ile Ser 
945                 950                 955                 960 


Lys Val Val Lys Thr Leu Leu Ser Asn Ile Val Arg Thr Glu Asp Glu 
                965                 970                 975     


Met Glu Thr Thr Ser Lys Phe Val Val Ser Cys Thr Gly Gly Ile Thr 
            980                 985                 990         


Asp Arg Leu Lys Lys Asp Trp Gly  Leu Asn Asp Val Trp  Asn Ser Ile 
        995                 1000                 1005             


Val Tyr  Ser Arg Phe Glu Arg  Leu Asn Glu Leu Thr  Lys Ser Asp 
    1010                 1015                 1020             


Ala Phe  Gly Arg Trp Glu Asn  Lys Glu Gly Lys Arg  Val Phe Gln 
    1025                 1030                 1035             


Thr Ser  Val Pro Leu Glu Leu  Gln Arg Gly Phe Asn  Lys Lys Arg 
    1040                 1045                 1050             


Ile Asp  His Arg His His Ala  Met Asp Ala Leu Val  Ile Ala Cys 
    1055                 1060                 1065             


Ala Ser  Arg Asn Ile Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Asn 
    1070                 1075                 1080             


Ser Pro  Lys Lys Arg Glu Asp  Leu Arg Gln Gln Leu  Cys Asp Lys 
    1085                 1090                 1095             


Asn Arg  Ile Ile Arg Lys Pro  Trp Glu Ser Phe Thr  Gln Asp Ala 
    1100                 1105                 1110             


Arg Ala  Ala Leu Glu Asp Val  Val Val Ser Phe Lys  Asn Tyr Val 
    1115                 1120                 1125             


Arg Val  Ile Asn Lys Thr Thr  Asn Tyr Tyr Glu Arg  Tyr Gly Ala 
    1130                 1135                 1140             


Asp Gly  Glu Lys Thr Ile Ala  Lys Gln Lys Asn Ser  Glu Met Trp 
    1145                 1150                 1155             


Ala Val  Arg Lys Pro Met His  Glu Ala Lys Tyr Tyr  Gly Arg Val 
    1160                 1165                 1170             


Asn Leu  Gln Arg Lys Thr Glu  Val Arg Leu Lys Ala  Ala Leu Glu 
    1175                 1180                 1185             


Asp Ile  Pro Ser Ile Cys Asn  Lys Glu Leu Arg Asp  Tyr Ile Asn 
    1190                 1195                 1200             


Arg Leu  Val Lys Ser Gly Leu  Asn Ala Arg Gln Leu  Leu Ala His 
    1205                 1210                 1215             


Phe Lys  Ser Thr Asp Tyr Cys  Trp Asn Lys Gln Asn  Val Asp Lys 
    1220                 1225                 1230             


Val Glu  Val Trp Arg Phe Ser  Asp Glu Lys Glu Pro  Leu Val Ala 
    1235                 1240                 1245             


Ile Arg  Glu Ser Leu Asp Thr  Ser Phe Asp Ala Lys  Arg Ile Asp 
    1250                 1255                 1260             


Thr Ile  Thr Asp Thr Gly Ile  Gln Lys Ile Leu Leu  Asn Tyr Leu 
    1265                 1270                 1275             


Glu Ala  Lys Gly Gly Asp Pro  Ala Ile Ala Phe Thr  Pro Glu Gly 
    1280                 1285                 1290             


Ile Ala  Glu Met Asn Gln Asn  Met Ala Leu Tyr Asn  Asn Gly Lys 
    1295                 1300                 1305             


Met His  Gln Pro Ile Leu Arg  Val Arg Leu Ser Lys  Lys Arg Gly 
    1310                 1315                 1320             


Lys Lys  Tyr Gln Val Gly Gln  Thr Gly Cys Lys Ala  Lys Gln Phe 
    1325                 1330                 1335             


Val Ile  Ser Glu Ser Gly Thr  Asn Leu Tyr Phe Ala  Ile Tyr Glu 
    1340                 1345                 1350             


Asp Glu  Asp Arg Asn Arg Thr  Tyr Asn Thr Ile Pro  Leu Asn Glu 
    1355                 1360                 1365             


Val Ala  Glu Arg Leu Lys Gln  Gly Leu Ser Pro Val  Pro Glu Lys 
    1370                 1375                 1380             


Asn Glu  Lys Glu Val Pro Leu  Lys Phe Tyr Leu Ser  Pro Asn Asp 
    1385                 1390                 1395             


Leu Val  Tyr Val Pro Thr Glu  Glu Glu Ile Leu Met  Lys Glu Cys 
    1400                 1405                 1410             


Lys Leu  Asn Lys Asn Arg Ile  Tyr Lys Phe Ile Asp  Ser Ser Gly 
    1415                 1420                 1425             


Thr Thr  Ala Asn Phe Val Pro  His Arg Ser Ala Ser  Leu Ile Tyr 
    1430                 1435                 1440             


Asn Val  Asp Lys Lys Leu Ala  Glu Ala Phe Cys Asn  Asp Glu Ile 
    1445                 1450                 1455             


Ile Gln  Asn Glu Tyr Gly Val  Gly Ser Pro Gln Ser  Lys Asn Gln 
    1460                 1465                 1470             


Lys Ala  Leu Thr Gly Glu Met  Ile Lys Ala Val Cys  Trp Lys Leu 
    1475                 1480                 1485             


Glu Val  Asp Arg Leu Gly Asn  Ile Ile Arg Val Ile  Lys 
    1490                 1495                 1500     


<210>  1302
<211>  1504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1302

Met Lys Asn Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Gly Asn Ile Asn Asp Asp Gly Ser Glu Gln Leu Val 
            20                  25                  30          


Lys Ile Gln Ala Ser Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Met 
        35                  40                  45              


Ile Gly Asp Phe Asn Lys Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg 
    50                  55                  60                  


Thr Arg Leu Arg Gly Ile Arg Arg Leu Ser Glu Arg Tyr Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Ile Leu Asp Ile Leu Gly Phe Leu Pro Ser 
                85                  90                  95      


His Phe Ala Arg Asp Leu Asp Arg Tyr Gly Lys Ile Val Lys Asp Lys 
            100                 105                 110         


Glu Pro Lys Leu Ala Trp Arg Lys Asn Glu Ala Gly Gln Phe Glu Phe 
        115                 120                 125             


Ile Phe Gln Asn Ser Phe Lys Glu Met Leu Glu Asp Phe Lys Leu Asn 
    130                 135                 140                 


His Pro Asn Leu Ile Thr Asp Asp Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Gly Leu Thr Ser Lys Ile Ser Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Glu Glu Asn Lys Asn Lys Gln Val Glu 
        195                 200                 205             


Phe Tyr Ala Leu Lys Val Ile Ala Val Glu Asp Ser Gly Glu Lys Lys 
    210                 215                 220                 


Gly Lys Asp Thr Trp Tyr Asn Val His Leu Glu Asn Gly Trp Ile Tyr 
225                 230                 235                 240 


Arg Arg Thr Ser Asn Met Pro Leu Asp Trp Val Gly Lys Thr Lys Glu 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Glu Lys Asp Gly Thr Pro Lys Lys Asp 
            260                 265                 270         


Lys Glu Gly Asn Ile Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp 
        275                 280                 285             


Trp Ser Leu Val Lys Lys Lys Thr Glu Ala Asp Ile Asp Gln Thr His 
    290                 295                 300                 


Lys Thr Val Gly Thr Tyr Ile Tyr Asp Thr Leu Leu Gly Asn Pro Ser 
305                 310                 315                 320 


Gln Lys Ile Arg Gly Arg Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Glu Glu Leu Lys Leu Ile Leu Asp Lys Gln Lys Glu Phe His Pro 
            340                 345                 350         


Glu Leu Gln Asp Arg Ala Leu Tyr Ala Ala Cys Val Glu Glu Leu Tyr 
        355                 360                 365             


Thr Ile Asn Glu Ala His Arg Asn Asn Ile Gly Asn Arg Asp Phe Thr 
    370                 375                 380                 


Tyr Leu Phe Leu Glu Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys Ser 
385                 390                 395                 400 


Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu Asn His Cys Leu 
                405                 410                 415     


Asp Lys Glu Thr Gly Glu Ile Lys Phe Phe Pro Val Lys Cys Ile Ala 
            420                 425                 430         


Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Ile Val 
        435                 440                 445             


Asn Leu Arg Ile Tyr Arg Lys Gly Leu Ala Gln Asp Val Asp Val Thr 
    450                 455                 460                 


Asn Glu Leu Leu Lys Thr Glu Glu Asp Tyr Val Ala Leu Phe Asp Trp 
465                 470                 475                 480 


Leu Asn Gly Lys Lys Glu Ile Asp Gln Lys Ala Phe Leu Lys Tyr Pro 
                485                 490                 495     


Pro Phe Gly Leu Lys Lys Glu Val Glu Asn Tyr Arg Trp Asn Tyr Val 
            500                 505                 510         


Gln Asp Lys Ser Tyr Pro Cys Asn Glu Thr Arg Ser Leu Met Leu Ala 
        515                 520                 525             


Arg Leu Glu Lys Cys Asp Ile Ser Ile Glu Phe Leu Thr Lys Glu Asn 
    530                 535                 540                 


Glu Glu Ala Leu Trp His Ile Leu Tyr Ser Val Glu Asp Lys Asn Glu 
545                 550                 555                 560 


Ile Val Lys Ala Leu Gln Lys Phe Ala Asp Lys His Asn Leu Asn Ala 
                565                 570                 575     


Ser Phe Val Asp Val Phe Gln Lys Phe Pro Ser Phe Lys Lys Glu Tyr 
            580                 585                 590         


Gly Ser Tyr Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg 
        595                 600                 605             


Met Gly Lys Tyr Trp Ser Glu Asp Ser Ile Asp Lys Gly Thr Arg Glu 
    610                 615                 620                 


Arg Met Asp Lys Ile Ile Thr Gly Glu Tyr Asp Glu Lys Ile Glu Asn 
625                 630                 635                 640 


Arg Val Arg Glu Lys Ala Ile His Leu Ala Asp Ile Ser Glu Phe Lys 
                645                 650                 655     


Gly Leu Pro Leu Trp Leu Val Cys Tyr Ile Val Tyr Gly Arg His Ser 
            660                 665                 670         


Glu Ser Lys Glu Ile Ala Lys Trp Glu Ser Pro Ala Asp Ile Asp Ser 
        675                 680                 685             


Tyr Leu Lys Ser Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu 
    690                 695                 700                 


Gln Ile Ile Ile Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Gln 
705                 710                 715                 720 


Val Gly Asn Ile Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys 
                725                 730                 735     


Asn Pro Ala Asp Lys Arg Arg Lys Met Thr Gln Gln Ile Ser Glu Asn 
            740                 745                 750         


Glu Asn Thr Asn Leu Arg Ile Lys Tyr Leu Leu Thr Glu Phe Leu Asn 
        755                 760                 765             


Pro Glu Tyr Glu Val Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln 
    770                 775                 780                 


Asp Ile Leu Arg Ile Tyr Glu Glu Gly Val Leu Asn Ser Val Ser Asn 
785                 790                 795                 800 


Leu Pro Glu Glu Ile Ser Asp Ile Leu Lys Lys Phe Thr Glu Thr Asp 
                805                 810                 815     


Leu Lys Lys Arg Pro Ser Arg Ser Glu Val Leu Arg Tyr Lys Leu Trp 
            820                 825                 830         


Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Glu Val Ile Pro Leu 
        835                 840                 845             


Gly Lys Leu Phe Thr Pro Ala Tyr Glu Ile Glu His Val Ile Pro Gln 
    850                 855                 860                 


Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val Ile Cys Glu Ala 
865                 870                 875                 880 


Glu Val Asn Lys Leu Lys Ser Asn Leu Leu Gly His Glu Phe Ile Val 
                885                 890                 895     


Lys His His Gly Glu Ile Val Glu Leu Pro Phe Gly Lys Lys Val Lys 
            900                 905                 910         


Ile Phe Thr Val Asp Glu Tyr Glu Gln Phe Val Lys Asp Asn Tyr Ser 
        915                 920                 925             


Arg Thr Arg Gly Lys Met Lys Lys Leu Leu Val Asp Asp Ile Pro Glu 
    930                 935                 940                 


Glu Phe Val Ala Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val 
945                 950                 955                 960 


Val Lys Ser Leu Leu Ser Asn Ile Val Arg Glu Lys Gly Glu Glu Glu 
                965                 970                 975     


Ala Ile Ser Lys Asn Val Ile Pro Cys Thr Gly Gly Val Thr Asp Arg 
            980                 985                 990         


Leu Lys Lys Asp Trp Gly Val Asn  Asp Val Trp Asn Lys  Ile Ile Leu 
        995                 1000                 1005             


Pro Arg  Phe Gln Arg Leu Asn  Glu Leu Thr Gly Thr  Asn Lys Phe 
    1010                 1015                 1020             


Thr Thr  Met Ser Leu Asn Asn  His Glu Ile Pro Ala  Met Pro Leu 
    1025                 1030                 1035             


Glu Leu  Gln Lys Gly Phe Asn  Lys Lys Arg Ile Asp  His Arg His 
    1040                 1045                 1050             


His Ala  Met Asp Ala Ile Ile  Ile Ala Cys Ala Asn  Arg Asn Ile 
    1055                 1060                 1065             


Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Thr Ala Lys  Ala Glu Leu 
    1070                 1075                 1080             


Ser Arg  Tyr Asp Leu Gln Arg  Leu Leu Cys Asp Lys  Ala Lys Thr 
    1085                 1090                 1095             


Asp Asp  Asn Gly Asn Tyr Lys  Trp Val Ile Arg Lys  Pro Trp Ala 
    1100                 1105                 1110             


Ser Phe  Thr Gln Asp Val His  Gly Val Leu Glu Asp  Ile Ile Val 
    1115                 1120                 1125             


Ser Phe  Lys Gln Asn Leu Arg  Val Ile Asn Lys Ala  Thr Asn His 
    1130                 1135                 1140             


Phe Leu  His Tyr Asn Glu Glu  Gly Lys Lys Ile Phe  Val Lys Gln 
    1145                 1150                 1155             


Glu Lys  Gly Asp Ser Trp Ala  Ile Arg Lys Ser Met  His Lys Asp 
    1160                 1165                 1170             


Thr Val  Phe Gly Glu Val Asn  Leu Arg Arg Ile Lys  Thr Val Ala 
    1175                 1180                 1185             


Leu Asn  Glu Ala Met Lys Asn  Pro Gln Ser Ile Val  Val Lys Asp 
    1190                 1195                 1200             


Phe Lys  Arg Lys Leu Leu Glu  Leu Leu Asn Leu Gly  Phe Asp Ala 
    1205                 1210                 1215             


Lys Arg  Ile Lys Lys Tyr Phe  Glu Asp Asn Arg Glu  Thr Trp Ser 
    1220                 1225                 1230             


Asp Ile  Asn Leu Ser Lys Ile  Thr Val Tyr Tyr Phe  Ser Lys Asp 
    1235                 1240                 1245             


Thr Lys  Asp Arg Phe Phe Ala  Thr Arg Lys Pro Leu  Asp Thr Ser 
    1250                 1255                 1260             


Phe Asp  Arg Lys Lys Ile Glu  Asn Asn Ile Thr Asp  Thr Gly Ile 
    1265                 1270                 1275             


Gln Lys  Ile Leu Leu Arg His  Leu Glu Leu Lys Asp  Asn Asn Pro 
    1280                 1285                 1290             


Asp Ile  Ala Phe Ser Pro Asp  Gly Ile Asp Glu Met  Asn Arg Asn 
    1295                 1300                 1305             


Ile Ile  Gln Leu Asn Asn Gly  Lys Tyr His Gln Pro  Ile Ile Lys 
    1310                 1315                 1320             


Val Arg  Trp Tyr Glu Gln Ala  Asp Lys Phe Ala Val  Gly Gln Thr 
    1325                 1330                 1335             


Gly Asn  Lys Ser Ser Lys Phe  Val Glu Ala Ala Lys  Gly Thr Asn 
    1340                 1345                 1350             


Leu Phe  Phe Ala Val Tyr Glu  Ser Asn Ile Leu Asp  Glu Lys Thr 
    1355                 1360                 1365             


Asn Thr  Ile Ile Lys Lys Arg  Asn Tyr Ala Thr Ile  Pro Leu Asn 
    1370                 1375                 1380             


Val Ala  Ile Glu Arg Gln Lys  Gln Gly Leu Ser Val  Ala Pro Glu 
    1385                 1390                 1395             


Glu Glu  Asn Gly Asn Asp Pro  Ile Phe Val Leu Ser  Pro Asn Asp 
    1400                 1405                 1410             


Leu Val  Tyr Leu Pro Thr Thr  Asp Glu Leu Ala Asn  Gly Val Val 
    1415                 1420                 1425             


Ala Gln  Pro Leu Asp Lys Glu  Arg Ile Tyr Lys Met  Val Ser Cys 
    1430                 1435                 1440             


Thr Gly  Asn Glu Gly His Phe  Ile Pro Ala Arg Ile  Ala Asn Pro 
    1445                 1450                 1455             


Ile Leu  Gln Thr Ile Glu Leu  Gly Ser Asn Asn Lys  Ala Gln Lys 
    1460                 1465                 1470             


Ala Trp  Thr Asp Glu Met Ile  Lys Glu Ile Cys Met  Pro Ile Lys 
    1475                 1480                 1485             


Val Asp  Arg Leu Gly Asn Ile  Leu Glu Ser Ser Ser  Leu His Lys 
    1490                 1495                 1500             


Lys 
    


<210>  1303
<211>  1504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1303

Met Lys Asn Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Gly Ser Val Asn Asp Asp Gly Ser Glu Gln Leu Val 
            20                  25                  30          


Lys Ile Gln Ala Ser Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Met 
        35                  40                  45              


Ile Gly Asp Phe Asn Lys Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg 
    50                  55                  60                  


Thr Arg Leu Arg Gly Val Arg Arg Leu Ser Glu Arg Tyr Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Ile Leu Asp Ile Leu Gly Phe Leu Pro Phe 
                85                  90                  95      


His Phe Ala Gln Asp Leu Asp Arg His Gly Lys Ile Val Lys Gly Lys 
            100                 105                 110         


Glu Pro Lys Leu Ala Trp Arg Lys Asn Glu Ala Gly Gln Phe Glu Phe 
        115                 120                 125             


Ile Phe Gln Asp Ser Phe Lys Glu Met Leu Glu Asp Phe Lys Leu Asn 
    130                 135                 140                 


Tyr Pro Asn Leu Ile Thr Asp Asp Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Gly Leu Thr Ser Lys Ile Ser Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Glu Glu Asn Lys Asp Lys Leu Val Glu 
        195                 200                 205             


Phe Tyr Ala Leu Lys Val Val Ala Val Glu Asp Ser Gly Glu Lys Lys 
    210                 215                 220                 


Gly Lys Asp Ile Trp Tyr Asn Val His Leu Glu Asn Gly Trp Val Tyr 
225                 230                 235                 240 


Arg Arg Thr Ser Asn Met Ser Leu Asp Trp Val Gly Lys Thr Lys Glu 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Glu Lys Asp Gly Thr Pro Lys Lys Asp 
            260                 265                 270         


Lys Glu Gly Asn Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp 
        275                 280                 285             


Trp Ser Leu Val Lys Lys Lys Thr Glu Ala Asp Ile Asp Gln Thr His 
    290                 295                 300                 


Lys Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Gly Asn Pro Ser 
305                 310                 315                 320 


Gln Lys Ile Arg Gly Arg Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Glu Glu Leu Lys Leu Ile Leu Glu Lys Gln Lys Glu Phe His Leu 
            340                 345                 350         


Glu Leu Gln Asp Arg Ala Leu Tyr Ala Ala Cys Ile Glu Glu Leu Tyr 
        355                 360                 365             


Pro Ile Asn Glu Ala His Arg Asn Asn Val Gly Asn Arg Asp Phe Thr 
    370                 375                 380                 


Tyr Leu Phe Leu Glu Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys Ser 
385                 390                 395                 400 


Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu Asn Gln Gly Leu 
                405                 410                 415     


Asp Lys Glu Thr Gly Glu Ile Lys Ser Phe Pro Val Lys Cys Ile Ala 
            420                 425                 430         


Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Ile Ile 
        435                 440                 445             


Asn Leu Arg Ile Tyr Arg Lys Asp Leu Ala Gln Asp Val Asp Val Thr 
    450                 455                 460                 


Asn Glu Leu Leu Lys Thr Glu Glu Asp Tyr Val Ala Leu Phe Asp Trp 
465                 470                 475                 480 


Leu Asn Gly Lys Lys Glu Ile Asp Gln Lys Ala Phe Leu Lys Tyr Pro 
                485                 490                 495     


Val Phe Gly Leu Lys Lys Glu Ile Glu Asn Tyr Arg Trp Asn Tyr Val 
            500                 505                 510         


Gln Asp Lys Pro Tyr Pro Cys Asn Glu Thr Arg Ser Leu Met Leu Ala 
        515                 520                 525             


Arg Leu Glu Lys Cys Asp Ile Ser Ile Glu Phe Leu Thr Lys Glu Asn 
    530                 535                 540                 


Glu Glu Ala Leu Trp His Ile Leu Tyr Ser Val Glu Asp Lys Asn Glu 
545                 550                 555                 560 


Ile Val Lys Ala Leu Gln Lys Phe Ala Asp Lys His Asp Leu Asn Ala 
                565                 570                 575     


Ser Phe Val Asp Val Phe Gln Lys Phe Pro Pro Phe Lys Lys Glu Tyr 
            580                 585                 590         


Gly Ser Tyr Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg 
        595                 600                 605             


Met Gly Lys Tyr Trp Ser Glu Asn Ser Ile Asp Lys Gly Thr Arg Glu 
    610                 615                 620                 


Arg Ile Asp Lys Ile Ile Thr Gly Glu Tyr Asp Glu Lys Ile Glu Asn 
625                 630                 635                 640 


Arg Val Arg Glu Lys Ala Ile His Leu Ala Asp Val Ser Asp Phe Lys 
                645                 650                 655     


Gly Leu Pro Leu Trp Leu Val Cys Tyr Ile Val Tyr Gly Arg His Ser 
            660                 665                 670         


Glu Ala Lys Glu Ile Ile Gln Trp Lys Ser Pro Ala Asp Ile Asp Ser 
        675                 680                 685             


Tyr Leu Arg Ser Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu 
    690                 695                 700                 


Gln Ile Ile Ile Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Gln 
705                 710                 715                 720 


Val Gly Asn Ile Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys 
                725                 730                 735     


Asn Pro Ala Asp Lys Arg Lys Lys Met Thr Gln Gln Met Ser Glu Asn 
            740                 745                 750         


Glu Asn Thr Asn Leu Arg Ile Lys Tyr Leu Leu Thr Glu Phe Leu Asn 
        755                 760                 765             


Pro Glu Tyr Glu Val Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln 
    770                 775                 780                 


Asp Ile Leu Arg Ile Tyr Glu Glu Gly Val Leu Asn Ser Val Ser Asp 
785                 790                 795                 800 


Leu Pro Glu Glu Ile Ser Asp Ile Leu Lys Lys Phe Thr Glu Thr Asp 
                805                 810                 815     


Leu Lys Lys Arg Pro Ser Arg Ser Glu Val Leu Arg Tyr Lys Leu Trp 
            820                 825                 830         


Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Glu Val Ile Pro Leu 
        835                 840                 845             


Gly Lys Leu Phe Thr Pro Ala Tyr Glu Ile Glu His Val Ile Pro Gln 
    850                 855                 860                 


Ser Cys Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val Ile Cys Glu Ala 
865                 870                 875                 880 


Glu Val Asn Lys Leu Lys Ser Asn Leu Leu Gly His Glu Phe Ile Val 
                885                 890                 895     


Lys His His Gly Glu Ile Val Glu Leu Pro Phe Gly Lys Lys Val Arg 
            900                 905                 910         


Ile Phe Thr Val Asp Glu Tyr Glu Gln Phe Val Lys Asp Asn Tyr Ser 
        915                 920                 925             


Arg Thr Arg Ser Lys Met Lys Lys Leu Leu Met Asp Asp Ile Pro Glu 
    930                 935                 940                 


Glu Phe Val Thr Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val 
945                 950                 955                 960 


Val Lys Ser Leu Leu Ser Asn Ile Val Arg Glu Lys Gly Glu Glu Glu 
                965                 970                 975     


Ala Ile Ser Lys Asn Val Ile Pro Cys Thr Gly Gly Val Thr Asp Arg 
            980                 985                 990         


Leu Lys Lys Asp Trp Gly Ile Asn  Glu Val Trp Asn Lys  Ile Ile Leu 
        995                 1000                 1005             


Pro Arg  Phe Gln Arg Leu Asn  Glu Leu Thr Gly Thr  Asn Lys Phe 
    1010                 1015                 1020             


Thr Thr  Lys Asn Val Gly Ile  Gln Glu Ile Pro Thr  Met Pro Leu 
    1025                 1030                 1035             


Glu Leu  Gln Lys Gly Phe Asn  Lys Lys Arg Ile Asp  His Arg His 
    1040                 1045                 1050             


His Ala  Met Asp Ala Ile Ile  Ile Ala Cys Ala Asn  Arg Asn Ile 
    1055                 1060                 1065             


Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Thr Ala Lys  Ala Glu Leu 
    1070                 1075                 1080             


Ser Arg  Tyr Asp Leu Gln Lys  Met Leu Cys Asp Lys  Ala Lys Thr 
    1085                 1090                 1095             


Asp Asn  Asn Gly Asn Tyr Lys  Trp Val Ile Arg Lys  Pro Trp Ala 
    1100                 1105                 1110             


Ser Phe  Thr Gln Asp Thr Tyr  Leu Ala Leu Glu Asn  Ile Ile Val 
    1115                 1120                 1125             


Ser Phe  Lys Gln Asn Leu Arg  Val Ile Asn Lys Ala  Thr Asn Arg 
    1130                 1135                 1140             


Phe Leu  His Tyr Asn Glu Glu  Gly Lys Lys Ile Phe  Val Lys Gln 
    1145                 1150                 1155             


Gly Lys  Gly Asp Asn Trp Ala  Ile Arg Lys Ser Met  His Lys Asp 
    1160                 1165                 1170             


Thr Val  Phe Gly Glu Val Asn  Leu Arg Arg Ile Lys  Thr Val Ala 
    1175                 1180                 1185             


Leu Asn  Glu Ala Met Lys Asn  Pro Gln Ser Ile Val  Val Lys Asp 
    1190                 1195                 1200             


Phe Lys  Arg Lys Leu Leu Glu  Leu Trp Asn Leu Gly  Phe Asp Ala 
    1205                 1210                 1215             


Lys Arg  Ile Lys Lys Tyr Phe  Glu Asp Asn Arg Glu  Thr Trp Ser 
    1220                 1225                 1230             


Asp Ile  Asn Leu Ser Lys Ile  Glu Val Tyr Tyr Phe  Ser Lys Asp 
    1235                 1240                 1245             


Thr Lys  Asp Arg Phe Phe Ala  Thr Arg Lys Pro Leu  Asp Thr Ser 
    1250                 1255                 1260             


Phe Asp  Arg Lys Lys Ile Glu  Asn Asn Ile Thr Asp  Thr Gly Ile 
    1265                 1270                 1275             


Gln Lys  Ile Leu Leu Arg His  Leu Glu Leu Lys Asp  Asn Asn Pro 
    1280                 1285                 1290             


Asp Ile  Ala Phe Ser Pro Asp  Gly Ile Asp Glu Met  Asn Arg Asn 
    1295                 1300                 1305             


Ile Ile  Gln Leu Asn Asn Gly  Lys Tyr His Gln Pro  Ile Ile Lys 
    1310                 1315                 1320             


Ile Arg  Trp Tyr Glu Gln Ala  Asp Lys Phe Ala Val  Gly Gln Thr 
    1325                 1330                 1335             


Gly Asn  Lys Ser Ser Lys Phe  Val Glu Ala Ala Lys  Gly Thr Asn 
    1340                 1345                 1350             


Leu Phe  Phe Ala Val Tyr Glu  Ser Asn Ile Leu Asp  Lys Lys Thr 
    1355                 1360                 1365             


Asn Thr  Ile Ile Lys Lys Arg  Asn Tyr Ala Thr Ile  Pro Leu Asn 
    1370                 1375                 1380             


Val Ala  Ile Glu Arg Gln Lys  Gln Gly Leu Pro Val  Ala Pro Glu 
    1385                 1390                 1395             


Asp Glu  Asn Gly Asn Asp Pro  Ile Phe Val Leu Ser  Pro Asn Asp 
    1400                 1405                 1410             


Leu Val  Tyr Leu Pro Thr Asp  Asp Glu Leu Ala Asn  Gly Ile Ile 
    1415                 1420                 1425             


Ala Gln  Pro Leu Asp Arg Gly  Arg Ile Tyr Lys Met  Val Ser Ser 
    1430                 1435                 1440             


Ser Gly  Glu Gln Cys Phe Phe  Ile Lys His Ile Val  Ala Asn Val 
    1445                 1450                 1455             


Leu Val  Asp Lys Phe Glu Phe  Ser Pro Leu Asn Lys  Met Glu Arg 
    1460                 1465                 1470             


Ala Leu  Thr Gly Glu Met Ile  Lys Met Ile Cys Ile  Pro Ile Lys 
    1475                 1480                 1485             


Val Asp  Arg Leu Gly Asn Val  Leu Glu Ser Ser Ser  Ser Tyr Lys 
    1490                 1495                 1500             


Lys 
    


<210>  1304
<211>  1504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1304

Met Lys Asn Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Gly Ser Val Asn Asp Asp Gly Ser Glu Gln Leu Val 
            20                  25                  30          


Lys Ile Gln Ala Ser Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Met 
        35                  40                  45              


Ile Gly Asp Phe Asn Lys Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg 
    50                  55                  60                  


Thr Arg Leu Arg Gly Val Arg Arg Leu Ser Glu Arg Tyr Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Ile Leu Asp Ile Leu Gly Phe Leu Pro Phe 
                85                  90                  95      


His Phe Ala Gln Asp Leu Asp Arg His Gly Lys Ile Val Lys Gly Lys 
            100                 105                 110         


Glu Pro Lys Leu Ala Trp Arg Lys Asn Glu Ala Gly Gln Phe Glu Phe 
        115                 120                 125             


Ile Phe Gln Asp Ser Phe Lys Glu Met Leu Glu Asp Phe Lys Leu Asn 
    130                 135                 140                 


His Pro Asn Leu Ile Thr Asp Asp Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Gly Leu Thr Ser Lys Ile Ser Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Glu Glu Asn Lys Asn Lys Leu Val Glu 
        195                 200                 205             


Phe Tyr Ala Leu Lys Val Val Ala Val Glu Asp Ser Gly Glu Lys Lys 
    210                 215                 220                 


Gly Lys Asp Ile Trp Tyr Asn Val His Leu Glu Asn Gly Trp Val Tyr 
225                 230                 235                 240 


Arg Arg Thr Ser Asn Met Pro Leu Asp Trp Val Gly Lys Thr Lys Glu 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Glu Lys Asp Gly Thr Pro Lys Lys Asp 
            260                 265                 270         


Lys Glu Gly Asn Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp 
        275                 280                 285             


Trp Ser Leu Val Lys Lys Lys Thr Glu Ala Asp Ile Asp Gln Thr His 
    290                 295                 300                 


Lys Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Gly Asn Pro Ser 
305                 310                 315                 320 


Gln Lys Ile Arg Gly Arg Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Glu Glu Leu Lys Gln Ile Leu Glu Lys Gln Lys Glu Phe His Leu 
            340                 345                 350         


Glu Leu Gln Asp Arg Ala Leu Tyr Ala Ala Cys Ile Glu Glu Leu Tyr 
        355                 360                 365             


Pro Ile Asn Glu Ala His Arg Asn Asn Val Gly Asn Arg Asp Phe Thr 
    370                 375                 380                 


Tyr Leu Phe Leu Glu Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys Ser 
385                 390                 395                 400 


Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu Asn Gln Gly Leu 
                405                 410                 415     


Asp Lys Glu Thr Gly Glu Ile Lys Ser Phe Pro Val Lys Cys Ile Ala 
            420                 425                 430         


Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Ile Ile 
        435                 440                 445             


Asn Leu Arg Ile Tyr Arg Lys Asp Leu Ala Gln Asp Val Asp Val Thr 
    450                 455                 460                 


Asn Glu Leu Leu Lys Thr Glu Glu Asp Tyr Val Ala Leu Phe Asp Trp 
465                 470                 475                 480 


Leu Asn Gly Lys Lys Glu Ile Asp Gln Lys Ala Phe Leu Lys Tyr Pro 
                485                 490                 495     


Val Phe Arg Leu Lys Lys Glu Ile Glu Asn Tyr Arg Trp Asn Tyr Val 
            500                 505                 510         


Gln Asp Lys Pro Tyr Pro Cys Asn Glu Thr Arg Ser Leu Met Leu Ala 
        515                 520                 525             


Arg Leu Glu Lys Cys Asp Ile Ser Ile Glu Phe Leu Thr Lys Glu Asn 
    530                 535                 540                 


Glu Glu Ala Leu Trp His Ile Leu Tyr Ser Val Glu Asp Lys Asn Glu 
545                 550                 555                 560 


Ile Val Lys Ala Leu Gln Lys Phe Ala Asp Lys His Asp Leu Asn Ala 
                565                 570                 575     


Ser Phe Val Asp Val Phe Gln Lys Phe Pro Pro Phe Lys Lys Glu Tyr 
            580                 585                 590         


Gly Ser Tyr Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg 
        595                 600                 605             


Met Gly Lys Tyr Trp Ser Glu Asn Ser Ile Asp Lys Gly Thr Arg Ala 
    610                 615                 620                 


Arg Ile Asp Lys Ile Ile Thr Gly Glu Tyr Asp Glu Lys Ile Glu Asn 
625                 630                 635                 640 


Arg Val Arg Glu Lys Ala Ile His Leu Ala Asp Val Ser Asp Phe Lys 
                645                 650                 655     


Gly Leu Pro Leu Trp Leu Val Cys Tyr Ile Val Tyr Gly Arg His Ser 
            660                 665                 670         


Glu Ala Lys Glu Ile Ile Gln Trp Lys Ser Pro Ala Asp Ile Asp Ser 
        675                 680                 685             


Tyr Leu Arg Ser Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu 
    690                 695                 700                 


Gln Ile Ile Ile Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Gln 
705                 710                 715                 720 


Val Gly Asn Ile Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys 
                725                 730                 735     


Asn Pro Ala Asp Lys Arg Lys Lys Met Thr Gln Gln Met Ser Glu Asn 
            740                 745                 750         


Glu Asn Thr Asn Leu Arg Ile Lys Tyr Leu Leu Thr Glu Phe Leu Asn 
        755                 760                 765             


Pro Glu Tyr Glu Val Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln 
    770                 775                 780                 


Asp Ile Leu Arg Ile Tyr Glu Glu Gly Val Leu Asn Ser Val Ser Asp 
785                 790                 795                 800 


Leu Pro Glu Glu Ile Ser Asp Ile Leu Lys Lys Phe Thr Glu Thr Asp 
                805                 810                 815     


Leu Lys Lys Arg Pro Ser Arg Ser Glu Ile Leu Arg Tyr Lys Leu Trp 
            820                 825                 830         


Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Glu Val Ile Pro Leu 
        835                 840                 845             


Gly Lys Leu Phe Thr Pro Ala Tyr Glu Ile Glu His Val Ile Pro Gln 
    850                 855                 860                 


Ser Cys Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val Ile Cys Glu Ala 
865                 870                 875                 880 


Glu Val Asn Lys Leu Lys Ser Asn Leu Leu Gly His Glu Phe Ile Val 
                885                 890                 895     


Lys His His Gly Glu Ile Val Glu Leu Pro Phe Gly Lys Lys Val Arg 
            900                 905                 910         


Ile Phe Thr Val Asp Glu Tyr Glu Gln Phe Val Lys Asp Asn Tyr Ser 
        915                 920                 925             


Arg Thr Arg Ser Lys Met Lys Lys Leu Leu Met Asp Asp Ile Pro Glu 
    930                 935                 940                 


Glu Phe Val Thr Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val 
945                 950                 955                 960 


Val Lys Ser Leu Leu Ser Asn Ile Val Arg Glu Lys Gly Glu Glu Glu 
                965                 970                 975     


Ala Ile Ser Lys Asn Val Ile Pro Cys Thr Gly Gly Val Thr Asp Arg 
            980                 985                 990         


Leu Lys Lys Asp Trp Gly Ile Asn  Glu Val Trp Asn Lys  Ile Ile Leu 
        995                 1000                 1005             


Pro Arg  Phe Gln Arg Leu Asn  Glu Leu Thr Gly Thr  Asn Lys Phe 
    1010                 1015                 1020             


Thr Thr  Lys Asn Val Gly Leu  Gln Glu Ile Pro Thr  Met Pro Leu 
    1025                 1030                 1035             


Glu Leu  Gln Lys Gly Phe Asn  Lys Lys Arg Ile Asp  His Arg His 
    1040                 1045                 1050             


His Ala  Met Asp Ala Ile Ile  Ile Ala Cys Ala Asn  Arg Asn Ile 
    1055                 1060                 1065             


Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Thr Ala Lys  Ala Glu Leu 
    1070                 1075                 1080             


Ser Arg  Tyr Asp Leu Gln Lys  Met Leu Cys Asp Lys  Ala Lys Thr 
    1085                 1090                 1095             


Asp Asn  Asn Gly Asn Tyr Lys  Trp Val Ile Arg Lys  Pro Trp Ala 
    1100                 1105                 1110             


Ser Phe  Thr Gln Asp Thr Tyr  Leu Ala Leu Glu Asn  Ile Ile Val 
    1115                 1120                 1125             


Ser Phe  Lys Gln Asn Leu Arg  Val Ile Asn Lys Ala  Thr Asn Arg 
    1130                 1135                 1140             


Phe Leu  His Tyr Asn Glu Glu  Gly Lys Lys Ile Phe  Val Lys Gln 
    1145                 1150                 1155             


Gly Lys  Gly Asp Asn Trp Ala  Ile Arg Lys Ser Met  His Lys Asp 
    1160                 1165                 1170             


Thr Val  Phe Gly Glu Val Asn  Leu Arg Arg Ile Lys  Thr Val Ala 
    1175                 1180                 1185             


Leu Asn  Glu Ala Met Lys Asn  Pro Gln Ser Ile Val  Val Lys Asp 
    1190                 1195                 1200             


Phe Lys  Arg Lys Leu Leu Glu  Leu Trp Asn Leu Gly  Phe Asp Ala 
    1205                 1210                 1215             


Lys Arg  Ile Lys Lys Tyr Phe  Glu Asp Asn Arg Glu  Thr Trp Ser 
    1220                 1225                 1230             


Asp Ile  Asn Leu Ser Lys Ile  Glu Val Tyr Tyr Phe  Ser Lys Asp 
    1235                 1240                 1245             


Thr Lys  Asp Arg Phe Phe Ala  Thr Arg Lys Pro Leu  Asp Thr Ser 
    1250                 1255                 1260             


Phe Asp  Arg Lys Lys Ile Glu  Asn Asn Ile Thr Asp  Thr Gly Ile 
    1265                 1270                 1275             


Gln Lys  Ile Leu Leu Arg His  Leu Glu Leu Lys Asp  Asn Asn Pro 
    1280                 1285                 1290             


Asp Ile  Ala Phe Ser Pro Asp  Gly Ile Asp Glu Met  Asn Arg Asn 
    1295                 1300                 1305             


Ile Ile  Gln Leu Asn Asn Gly  Lys Tyr His Gln Pro  Ile Ile Lys 
    1310                 1315                 1320             


Val Arg  Trp Tyr Glu Gln Ala  Asp Lys Phe Ala Val  Gly Gln Thr 
    1325                 1330                 1335             


Gly Asn  Lys Phe Ser Lys Phe  Val Glu Ala Ala Lys  Gly Thr Asn 
    1340                 1345                 1350             


Leu Phe  Phe Ala Val Tyr Glu  Ser Asn Ile Leu Asp  Lys Lys Thr 
    1355                 1360                 1365             


Asn Thr  Ile Ile Lys Lys Arg  Asn Tyr Ala Thr Ile  Pro Leu Asn 
    1370                 1375                 1380             


Val Ala  Ile Glu Arg Gln Lys  Gln Gly Leu Pro Val  Ala Pro Glu 
    1385                 1390                 1395             


Asp Glu  Asn Gly Asn Asp Pro  Ile Phe Val Leu Ser  Pro Asn Asp 
    1400                 1405                 1410             


Leu Val  Tyr Leu Pro Thr Asp  Asp Glu Leu Ala Asn  Gly Ile Ile 
    1415                 1420                 1425             


Ala Gln  Pro Leu Asp Arg Gly  Arg Ile Tyr Lys Met  Val Ser Ser 
    1430                 1435                 1440             


Ser Gly  Glu Gln Cys Phe Phe  Ile Lys His Ile Val  Ala Asn Val 
    1445                 1450                 1455             


Leu Val  Asp Lys Phe Glu Phe  Ser Pro Leu Asn Lys  Met Glu Arg 
    1460                 1465                 1470             


Ala Leu  Thr Gly Glu Met Ile  Lys Met Ile Cys Ile  Pro Ile Lys 
    1475                 1480                 1485             


Val Asp  Arg Leu Gly Asn Val  Leu Glu Ser Ser Ser  Ser His Lys 
    1490                 1495                 1500             


Lys 
    


<210>  1305
<211>  1504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1305

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Asn Asp Phe Asn Arg Lys Glu Gly Lys Ile Glu Ala 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Lys Phe 
        35                  40                  45              


Asp Ser Gly Val Thr Val Ser Gln Thr Ala Ala Arg Thr Asn Tyr Arg 
    50                  55                  60                  


Gly Ile Arg Arg Leu Tyr Gln Arg Asp Asn Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Asn Ile Leu Gly Phe Leu Pro Lys His Tyr Ala Glu 
                85                  90                  95      


Ser Ile Asp Phe Glu Asp Arg Leu Gly Gln Phe Lys Pro Glu Thr Glu 
            100                 105                 110         


Val Lys Leu Pro Tyr Arg Lys Asp Ser Asp Asp Asn Tyr His Phe Ile 
        115                 120                 125             


Phe Gln Glu Ser Phe Asn Glu Met Val Glu Glu Phe Gln Phe Lys Asn 
    130                 135                 140                 


Pro Ser Leu Phe Tyr Thr Lys Ala Asn Gly Glu Gln Ser Lys Ile Pro 
145                 150                 155                 160 


Tyr Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Lys Lys 
                165                 170                 175     


Ile Ser Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys 
            180                 185                 190         


Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Ala Asp Ser Lys Asp 
        195                 200                 205             


Lys Leu Glu Glu Tyr His Ala Leu Lys Val Ser Asp Val Gln Ala Thr 
    210                 215                 220                 


Glu Asp Thr Asn Ala Lys Gly Thr Trp Tyr Asn Val Ile Leu Glu Asn 
225                 230                 235                 240 


Gly Trp Ile Tyr Arg Arg Gln Ser Lys Asp Ser Leu Phe Asp Trp Lys 
                245                 250                 255     


Gly Lys Thr Arg Glu Phe Ile Val Thr Thr Ser Leu Glu Lys Gly Gly 
            260                 265                 270         


Ala Val Lys Ile Asp Lys Glu Gly Asn Glu Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Val Asp Ser Glu Lys Asp Trp Ile Ala Ile Lys Lys Lys Thr Glu Asn 
    290                 295                 300                 


Asp Leu Ala Asn Ala Gly Thr Thr Val Gly Val Tyr Ile Tyr Glu Thr 
305                 310                 315                 320 


Leu Leu Arg Asn Pro Asp Gln Lys Ile Arg Gly Lys Leu Ile Lys Thr 
                325                 330                 335     


Ile Glu Arg Lys Tyr Tyr Leu Glu Glu Leu Lys Ala Ile Leu Ala Lys 
            340                 345                 350         


Gln Ile Gln Glu His Ala Glu Leu Gln Asn Arg Ser Leu Tyr Lys Asp 
        355                 360                 365             


Cys Ile Glu Glu Leu Tyr Gln Gly Asn Glu Ala His Lys Asn Asn Ile 
    370                 375                 380                 


Lys Glu Asn Gly Phe Asp Tyr Leu Phe Leu Glu Asp Ile Ile Phe Tyr 
385                 390                 395                 400 


Gln Arg Pro Leu Lys Ser Lys Lys Ser Thr Ile Ser Asn Cys Pro Tyr 
                405                 410                 415     


Glu Ser Arg Val Phe Ile Lys Asp Gly Lys Lys Glu Ile Gln Pro Ile 
            420                 425                 430         


Lys Cys Ile Ser Lys Ser Asn Pro Ile Tyr Gln Glu Phe Arg Leu Trp 
        435                 440                 445             


Gln Phe Ile His Asn Leu Lys Ile Tyr Glu Lys Glu Ala Ile Asp Asp 
    450                 455                 460                 


Gly Lys Thr Val Ile Asn Lys Asp Val Thr Thr Glu Phe Leu Asn Thr 
465                 470                 475                 480 


Gln Glu Ala Tyr Ala Ala Leu Phe Glu Phe Leu Asn Asp Lys Ala Glu 
                485                 490                 495     


Ile Ser Gln Asn Ala Leu Leu Lys Tyr Phe Lys Leu Ser Thr Asn Thr 
            500                 505                 510         


His Arg Trp Asn Asn Val Glu Asp Lys Lys Tyr Pro Cys Asn Glu Thr 
        515                 520                 525             


Arg Ser Glu Phe Ile Lys Arg Leu Ser Lys Met Asp Gly Ile Asp Leu 
    530                 535                 540                 


Gly Lys Thr Leu Ser Pro Ala Phe Glu Lys Ser Leu Trp His Ile Ile 
545                 550                 555                 560 


Tyr Ser Val Lys Asp Lys Lys Gln Tyr Val Arg Ala Leu Glu Thr Phe 
                565                 570                 575     


Ala Met Lys Asn Gly Leu Asp Ile Asn Gln Phe Arg Glu Asn Phe Glu 
            580                 585                 590         


Lys Phe Lys Pro Tyr Asp Ser Ala Tyr Gly Ala Tyr Ser Glu Lys Ala 
        595                 600                 605             


Leu Lys Lys Val Leu Pro Leu Ile Arg Arg Gly Thr Tyr Trp Lys Lys 
    610                 615                 620                 


Asp Ala Ile Pro Ala Ala Val Gln Glu Arg Ile Val Asn Ile Met Lys 
625                 630                 635                 640 


Arg Ile Glu Ser Val Asp Phe Asn Leu Glu Lys Ile Glu Asn Phe Ala 
                645                 650                 655     


Asp Asp Asp Ile Pro Lys Gln Leu Leu Lys Ser Phe Ala Lys Gly Lys 
            660                 665                 670         


Asp Pro Met Gln Gly Leu Asn Thr Tyr Gln Ala Cys Tyr Ala Val Tyr 
        675                 680                 685             


Asn Arg His Ser Glu Val Ser Asp Ile Gln Leu Trp Lys Ser Pro Glu 
    690                 695                 700                 


Asp Ile Thr Lys Tyr Leu Asp Ser Phe Lys Gln His Ser Leu Arg Asn 
705                 710                 715                 720 


Pro Ile Val Glu Gln Val Val Met Glu Thr Leu Arg Val Val Arg Asp 
                725                 730                 735     


Ile Trp Gln Tyr Tyr Gly Asn Gly Ala Lys Asp Phe Phe Ser Glu Ile 
            740                 745                 750         


His Val Glu Leu Gly Arg Glu Met Lys Asn Asp Lys Lys Thr Arg Glu 
        755                 760                 765             


Arg Ile Ser Lys Ser Ile Thr Glu Asn Glu Asn Thr Asn Glu Arg Ile 
    770                 775                 780                 


Lys Asn Ile Leu Lys Glu Leu Met Gly Asp Glu Ala Leu Gln Gly Asp 
785                 790                 795                 800 


Ile Arg Pro Tyr Ser Lys Gly His Gln Asp Ile Leu Lys Leu Tyr Glu 
                805                 810                 815     


Glu Gly Val Phe Ala Asn Ala Pro Glu Thr Tyr Lys Asn Ile Lys Ile 
            820                 825                 830         


Asp Asp Ile Glu Lys Ile Arg Lys Asn Asn Ser Pro Ser Lys Ser Asp 
        835                 840                 845             


Ile Val Lys Tyr Lys Leu Trp Leu Glu Gln Gly Tyr Ile Ser Pro Tyr 
    850                 855                 860                 


Thr Gly Ser Pro Ile Pro Leu Ser Arg Leu Phe Thr Thr Asp Tyr Gln 
865                 870                 875                 880 


Ile Glu His Ile Ile Pro Arg Ser Arg Tyr Phe Asp Asp Ser Met Gly 
                885                 890                 895     


Asn Lys Ile Ile Cys Glu Ser Ala Val Asn Glu Glu Lys Thr Asn Lys 
            900                 905                 910         


Thr Ala Tyr Glu Phe Ile Lys Glu Asn Ala Gly Arg Ile Ile Asp Leu 
        915                 920                 925             


Gly Gln Gly Lys Gln Val Pro Leu Phe Asp Leu Glu Ala Tyr Glu Ser 
    930                 935                 940                 


His Cys Arg Gly Tyr Phe Lys Gly Asn Thr Lys Lys Leu Lys Phe Leu 
945                 950                 955                 960 


Leu Ser Glu Asp Ile Pro Glu Gly Phe Ile Asn Arg Gln Leu Asn Asp 
                965                 970                 975     


Ser Arg Tyr Ile Ser Lys Val Val Thr Ser Leu Leu Ser Asn Val Val 
            980                 985                 990         


Arg Glu Glu Gly Glu Gln Glu Ala  Thr Ser Lys Asn Leu  Val Pro Leu 
        995                 1000                 1005             


Val Gly  Thr Ile Thr Ser Lys  Leu Lys Lys Asp Trp  Gly Leu Glu 
    1010                 1015                 1020             


Ala Ile  Trp Asp Asp Leu Ile  Ala Pro Arg Phe Glu  Arg Met Asn 
    1025                 1030                 1035             


Gln Leu  Thr Lys Ser Thr Asp  Phe Arg Lys Glu Val  Ile Asp Gly 
    1040                 1045                 1050             


His Gly  Asn Arg Tyr Phe Ile  Asn Thr Val Pro Asp  Asp Ile Lys 
    1055                 1060                 1065             


Lys Gly  Phe Ser Lys Lys Arg  Ile Asp His Arg His  His Ala Leu 
    1070                 1075                 1080             


Asp Ala  Leu Val Ile Ala Cys  Thr Thr Lys Asp His  Val Asn Tyr 
    1085                 1090                 1095             


Ile Thr  Ser Leu Asn Thr Gln  Arg Asn Asn Arg Ser  Leu Val Ser 
    1100                 1105                 1110             


Lys Leu  Arg Lys Ile Glu Glu  Lys Thr Ile Lys Asp  Lys Arg Thr 
    1115                 1120                 1125             


Gly Gln  Pro Lys Thr Ile Lys  Ile Ala Lys Ala Tyr  His Lys Pro 
    1130                 1135                 1140             


Trp Pro  Asn Phe Thr Lys Glu  Thr Phe Glu Lys Leu  Ser Lys Thr 
    1145                 1150                 1155             


Val Val  Ser Phe Lys Gln Asn  Gln Arg Ile Ile Asn  Lys Thr Asn 
    1160                 1165                 1170             


Asn Lys  Thr Trp Gly Trp Glu  Lys Val Asp Gly Val  Met Lys Lys 
    1175                 1180                 1185             


Val Leu  Lys Lys Gln Glu Lys  Arg Asn Trp Ala Ile  Arg Lys Pro 
    1190                 1195                 1200             


Ile His  Lys Glu Thr Tyr Tyr  Gly Lys Val Lys Gly  Phe Asp Ser 
    1205                 1210                 1215             


Pro Lys  Gly Lys Ile Thr Thr  Ala Thr Arg Lys Ser  Leu Arg Glu 
    1220                 1225                 1230             


Ile Thr  Asn Glu Arg Gln Leu  Asn Thr Ile Thr Asp  Ser Gly Ile 
    1235                 1240                 1245             


Lys Lys  Ile Leu Asn Asn His  Leu Lys Thr Phe Ile  Asp Glu Ser 
    1250                 1255                 1260             


Gly Lys  Ile Asn Phe Glu Pro  Ala Phe Asn Gln Asp  Gly Ile Asp 
    1265                 1270                 1275             


Leu Leu  Asn Lys Asn Ile Gln  Gln Leu Asn Glu Gly  Lys Asn His 
    1280                 1285                 1290             


Gln Pro  Ile Tyr Lys Val Arg  Met Tyr Thr His Asn  Thr Met Phe 
    1295                 1300                 1305             


Ser Val  Gly Asp Glu Gly Asn  Lys Ser Thr Lys Tyr  Val Ile Pro 
    1310                 1315                 1320             


Glu Ser  Gly Thr Asn Leu Phe  Phe Asn Val Tyr Trp  Asp Glu Glu 
    1325                 1330                 1335             


Asn Asn  Lys Arg Asn Phe Glu  Thr Val Pro Leu Asn  Arg Val Ile 
    1340                 1345                 1350             


Ala Phe  Gln Lys Gln Thr Ser  His Ile Pro Arg Lys  Asp Arg Thr 
    1355                 1360                 1365             


Asp Val  Pro Ile Asp Asn Thr  Leu Gly Arg Phe Leu  Phe Thr Leu 
    1370                 1375                 1380             


Ser Pro  Asp Asp Leu Val Tyr  Leu Pro Thr Asp Glu  Glu Ser Glu 
    1385                 1390                 1395             


Ser Phe  Thr Thr Phe Glu Ile  Asp Ser Leu Asn Ser  Lys Gln Val 
    1400                 1405                 1410             


Ala Asn  Ile Tyr Lys Phe Val  Asp Gly Ser Gly Thr  Thr Gly Asn 
    1415                 1420                 1425             


Phe Val  Pro Ala Glu Ala Ala  Arg Val Ile Ala Asn  Ile Lys Asp 
    1430                 1435                 1440             


Lys Lys  Val Arg Ser Asn Phe  Cys Lys Arg Asn Asn  Leu Ser Asp 
    1445                 1450                 1455             


Lys Gln  Leu Ile Lys Asn Glu  Phe Gly Leu Gly Ser  Pro Gln Leu 
    1460                 1465                 1470             


Lys Asn  Gln Asn Ser Leu Asp  Gly Arg Gln Ile Lys  Ser Ile Cys 
    1475                 1480                 1485             


Trp Lys  Leu Glu Ile Asn Arg  Leu Gly Lys Ile Ile  Lys Val Ile 
    1490                 1495                 1500             


Lys 
    


<210>  1306
<211>  1504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1306

Met Lys Lys Val Leu Gly Leu Asp Ile Gly Thr Asn Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Gln Asn Asp Lys Gly Glu Phe Leu Ser Ile Glu Lys 
            20                  25                  30          


Ile Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Met Asp Lys Phe 
        35                  40                  45              


Gly Ala Gly Gln Thr Glu Ser Ser Thr Ala Gln Arg Thr Gln Tyr Arg 
    50                  55                  60                  


Gly Val Arg Arg Leu Arg Glu Arg Ser Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Ile Leu His Phe Leu Pro Gln His Tyr Asp Ala 
                85                  90                  95      


Ala Ile Gly Trp Asn Lys Lys Glu Asn Lys Thr Tyr Gly Lys Phe Leu 
            100                 105                 110         


Pro Gly Val Glu Val Asn Ile Pro Trp Val Ala Thr Asp Gln Gly His 
        115                 120                 125             


Gln Phe Ile Phe Met Asp Ser Tyr Ala Glu Met Leu Lys Asp Leu Lys 
    130                 135                 140                 


Gln His Gln Pro His Leu Phe Glu Lys Ser Ser Thr Pro Val Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Ala Ile 
                165                 170                 175     


Ser Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Val Glu Asp Asn Thr Lys Arg 
        195                 200                 205             


Glu Glu Phe Tyr Ala Leu Lys Val Val Lys Val Glu Leu Asp Lys Asp 
    210                 215                 220                 


Asn Thr Ser Ala Lys Thr Trp Tyr Lys Val His Leu Glu Asn Gly Phe 
225                 230                 235                 240 


Ile Tyr Pro Cys Pro Ser Asp Thr Pro Ile Asp Ser Trp Val Gly Met 
                245                 250                 255     


Val Lys Asp Phe Ile Val Thr Thr Thr Leu Glu Lys Asp Gly Ser Glu 
            260                 265                 270         


Lys Arg Thr Ala Asp Gly Glu Ile Lys Arg Ser Phe Arg Ala Pro Lys 
        275                 280                 285             


Glu Asp Asp Trp Thr Leu Leu Lys Lys Lys Thr Glu Ser Glu Leu Glu 
    290                 295                 300                 


Lys Ser Gly Lys Gln Val Gly Ala Phe Ile Tyr Asp Thr Leu Leu Ser 
305                 310                 315                 320 


Asn Pro Asp Gln Lys Ile Ile Gly Gly Leu Ile Lys Thr Ile Glu Arg 
                325                 330                 335     


Lys Tyr Tyr Lys Lys Glu Leu Glu Gln Ile Leu Glu Thr Gln Gln Lys 
            340                 345                 350         


Phe Ile Ala Glu Leu Ser Asp Lys Lys Leu Phe Gln Ala Cys Ala Glu 
        355                 360                 365             


Glu Leu Tyr Pro Leu Asn Asp Ser His Arg Glu Asn Leu Leu Glu Arg 
    370                 375                 380                 


Asp Phe Lys His Leu Phe Val Gln Asp Ile Ile Phe Tyr His Arg Pro 
385                 390                 395                 400 


Leu Lys Thr Lys Lys Ser Leu Ile Ser Asn Cys Thr Tyr Glu Tyr Arg 
                405                 410                 415     


Thr Phe Tyr Lys Asn Gly Glu Lys Gln Ile Ser Pro Ile Lys Cys Ile 
            420                 425                 430         


Ala Lys Ser Asn Pro Leu Phe Gln Glu Phe Arg Val Trp Gln Phe Val 
        435                 440                 445             


Gln Asn Leu Arg Ile Leu Gln Arg Gln Arg Ser Glu Gly Asp Ser Ile 
    450                 455                 460                 


Lys Tyr Asn Val Asp Val Thr Ala Glu Phe Leu Lys Asp Glu Asn Asp 
465                 470                 475                 480 


Ile Ala Asn Leu Phe Thr Trp Leu Asn Glu Arg Lys Val Ile Lys Gln 
                485                 490                 495     


Asp Gln Leu Leu Lys Ser Tyr Phe Lys Leu Lys Val Asn Lys Glu Thr 
            500                 505                 510         


Lys Glu Leu Asp Tyr Arg Trp Asn Tyr Val Asp Asp Lys Asn Lys Glu 
        515                 520                 525             


Tyr Pro Cys Asn Ala Thr Arg Thr His Ile Leu Ser Phe Leu Glu Lys 
    530                 535                 540                 


Cys Lys Glu Glu Lys Arg Ile Glu Asp Asp Arg Phe Val Tyr Ser Leu 
545                 550                 555                 560 


Trp His Leu Leu Tyr Ser Val Val Glu Pro Ile Glu Leu Arg Gly Ala 
                565                 570                 575     


Leu Ala Lys Phe Ala Glu Arg Glu His Leu His Pro Ser Phe Val Asp 
            580                 585                 590         


Val Phe Ala Gln Phe Pro Pro Leu Glu Lys Glu Tyr Gly Ser Tyr Ser 
        595                 600                 605             


Glu Lys Ala Ile Lys Arg Leu Leu Pro Leu Met Arg Met Gly Asp Tyr 
    610                 615                 620                 


Trp His Glu Asp Lys Ile Asp Val Lys Thr Lys Gln Arg Ile Gln Arg 
625                 630                 635                 640 


Ile Ile Asp Gly Glu Ala Asp Asp Asp Ile Thr His Arg Ser Arg Glu 
                645                 650                 655     


Lys Val Glu Asn Leu Arg Ser Ile Thr Asp Phe Lys Gly Leu Pro Leu 
            660                 665                 670         


Trp Lys Ala Gly Tyr Val Val Tyr Asn Arg His Ser Glu Ser Gly Glu 
        675                 680                 685             


Leu Thr Lys Trp Lys Thr Pro His Glu Leu Ser Asn Tyr Ile Gln Asn 
    690                 695                 700                 


Glu Phe Lys Gln Tyr Ser Leu Arg Asn Pro Ile Val Glu Gln Ile Val 
705                 710                 715                 720 


Leu Glu Thr Leu Arg Val Val His Asp Leu Trp Val Lys His Gly Asp 
                725                 730                 735     


Phe Ser Glu Ile His Leu Glu Leu Gly Arg Asp Ile Lys Asn Ser Ser 
            740                 745                 750         


Asp Lys Arg Lys Arg Met Ala Glu Arg Gln Gln Glu Asn Glu Asn Ser 
        755                 760                 765             


Arg Gln Arg Val Leu Glu Met Leu Ile Asp Leu Lys Lys Glu Ala Ala 
    770                 775                 780                 


Phe Ala Asp Leu Asn Pro Tyr Ser Pro Ser Gln Gln Glu Lys Leu Arg 
785                 790                 795                 800 


Ile Val Glu Asp Gly Val Leu Arg Ser Asn Ile Glu Ile Pro Lys Asp 
                805                 810                 815     


Ile Glu Lys Ile Thr Lys Ser Ala Lys Pro Thr Ser Arg Glu Leu Thr 
            820                 825                 830         


Lys Tyr Arg Leu Trp Leu Asp Gln Lys Tyr Arg Ser Pro Tyr Thr Gly 
        835                 840                 845             


Gln Pro Ile Ser Leu Ala Gln Leu Phe Thr Ser Lys Tyr Glu Ile Glu 
    850                 855                 860                 


His Val Ile Pro Gln Ala Lys Tyr Phe Asp Asp Ser Phe Thr Asn Lys 
865                 870                 875                 880 


Val Ile Cys Glu Ser Glu Val Asn Lys Glu Lys Gly Ser Met Leu Ala 
                885                 890                 895     


His Glu Phe Ile Leu Lys Asn Gly Gly Ser Thr Val Asn Val Gly Gly 
            900                 905                 910         


Arg Gln Val Gln Val Leu Lys Asn Asp Ala Tyr Val Glu Leu Ile Asn 
        915                 920                 925             


Arg Leu Tyr Gly Lys Asn Asn Pro Val Lys Ala Lys Lys Leu Leu Ala 
    930                 935                 940                 


Glu Asp Ile Asp Glu Val Val Gly Asp Phe Ser Glu Arg Gln Leu Asn 
945                 950                 955                 960 


Asp Thr Arg Tyr Ile Ser Arg Val Val Gln Lys Leu Leu Ser Asn Ile 
                965                 970                 975     


Val Arg Glu Glu Asp Glu Gln Glu Ser Thr Ser Lys His Val Ile Gly 
            980                 985                 990         


Cys Ser Gly Gln Val Thr Thr Ala  Leu Lys Lys Asp Trp  Gly Leu Asn 
        995                 1000                 1005             


Asp Ile  Trp Asn Lys Ile Ile  Gln Pro Arg Phe Glu  Arg Leu Asn 
    1010                 1015                 1020             


Glu Met  Thr Ala Thr Glu Asp  Tyr Gly Val Trp Gln  Ser Pro Asn 
    1025                 1030                 1035             


His Phe  Gln Ile Arg Val Pro  Glu Glu Ile Gln Lys  Gly Phe Thr 
    1040                 1045                 1050             


Lys Lys  Arg Ile Asp His Arg  His His Ala Met Asp  Ala Leu Val 
    1055                 1060                 1065             


Ile Ala  Cys Ala Thr Arg Ser  His Ile Asn Tyr Ile  Ser Asn Arg 
    1070                 1075                 1080             


Ser Gly  Gln Asp Arg Asn Ala  Lys Phe Glu Thr Thr  Arg Thr Asp 
    1085                 1090                 1095             


Leu Arg  Ser Lys Leu Cys Tyr  Lys Glu Asn Ser Ser  Gly Asn Asn 
    1100                 1105                 1110             


Tyr Asn  Tyr Ile Phe Asn Lys  Pro Trp Asp Thr Phe  Thr Gln Asp 
    1115                 1120                 1125             


Ala His  Arg Ala Leu Glu Asn  Ile Val Val Ser His  Lys Gln Asn 
    1130                 1135                 1140             


Leu Arg  Val Leu Thr Lys Thr  Asn Asn Tyr Thr Gln  Phe Ile Asn 
    1145                 1150                 1155             


Lys Asp  Gly Lys Lys Asp Leu  Gln Lys Gln Val Lys  Gly Asp His 
    1160                 1165                 1170             


Phe Ala  Val Arg Lys Pro Met  His Gln Glu Thr Ile  Tyr Gly Arg 
    1175                 1180                 1185             


Val Ser  Ile Arg Ser Ile Lys  Glu Val Ala Leu Lys  Lys Ala Leu 
    1190                 1195                 1200             


Glu Asn  Trp Lys Gln Ile Val  Asp Arg Thr Leu Lys  Asp Lys Ile 
    1205                 1210                 1215             


Arg Glu  Leu Ile Gly Ala Tyr  Gly Lys Tyr Asp Ala  Gln Thr Ile 
    1220                 1225                 1230             


Leu Lys  Tyr Phe Lys Asp Arg  Asp Tyr Lys Phe Asn  Asn Leu Lys 
    1235                 1240                 1245             


Ile Asn  Arg Val Glu Val Tyr  Glu Tyr Asp His Asn  Cys Ala Ala 
    1250                 1255                 1260             


Val Arg  Lys Ser Ile Gly Asp  Asp Leu Thr Ile Lys  Gln Ile Glu 
    1265                 1270                 1275             


Arg Ile  Thr Asp Glu Ser Ile  Arg Lys Ile Leu Met  Asn His Leu 
    1280                 1285                 1290             


Ser Asn  Tyr Asp Asp Asn Leu  Lys Glu Ala Phe Ser  Pro Glu Gly 
    1295                 1300                 1305             


Leu Met  Glu Met Asn Lys Asn  Ile Lys Glu Leu Asn  Gly Gly Lys 
    1310                 1315                 1320             


Asn His  Gln Pro Ile Tyr Lys  Val Arg Leu Phe Glu  Asp Leu Gly 
    1325                 1330                 1335             


Ser Lys  Phe Pro Leu Gly Val  Ser His Asn Asn Pro  Asp Lys Tyr 
    1340                 1345                 1350             


Ala Ile  Ala Ala Lys Gly Thr  Asn Leu Phe Phe Gly  Ile Tyr Glu 
    1355                 1360                 1365             


Asp Gln  Glu Gly Lys Arg Met  Phe Asp Ser Ile Pro  Leu Asn Ile 
    1370                 1375                 1380             


Val Ile  Asp Arg Leu Lys Glu  Lys Lys Ser Pro Val  Pro Glu Thr 
    1385                 1390                 1395             


Tyr Asn  Glu Ser Lys Leu Lys  Phe His Leu Ser Pro  Asn Asp Leu 
    1400                 1405                 1410             


Val Tyr  Val Pro Thr Asp Glu  Glu Gln Lys Gln Pro  Glu Leu Val 
    1415                 1420                 1425             


Asp Val  Asp His Leu Ser Asp  Glu Gln Lys Gly Arg  Val Tyr Lys 
    1430                 1435                 1440             


Ala Val  Ser Phe Thr Gly Asn  Arg Ala Phe Phe Val  Asn His Arg 
    1445                 1450                 1455             


Val Ser  Lys Val Ile His Asp  Tyr Val Glu Phe Gly  Thr Lys Asn 
    1460                 1465                 1470             


Lys Ile  Glu Asn Asp Asp Asp  Lys Arg Ser Leu Lys  His Ile Cys 
    1475                 1480                 1485             


Trp Lys  Leu Lys Val Asn Arg  Leu Gly Glu Ile Thr  Asp Val Lys 
    1490                 1495                 1500             


Arg 
    


<210>  1307
<211>  1504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1307

Met Lys Asn Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Gly Ser Val Asn Asp Asp Gly Ser Glu Gln Leu Val 
            20                  25                  30          


Lys Ile Gln Ala Ser Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Met 
        35                  40                  45              


Ile Gly Asp Phe Asn Lys Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg 
    50                  55                  60                  


Thr Arg Leu Arg Gly Val Arg Arg Leu Ser Glu Arg Tyr Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Ile Leu Asp Ile Leu Gly Phe Leu Pro Phe 
                85                  90                  95      


His Phe Ala Gln Asp Leu Asp Arg His Gly Lys Ile Val Lys Gly Lys 
            100                 105                 110         


Glu Pro Lys Leu Ala Trp Arg Lys Asn Glu Ala Gly Gln Phe Glu Phe 
        115                 120                 125             


Ile Phe Gln Asp Ser Phe Lys Glu Met Leu Glu Asp Phe Lys Leu Asn 
    130                 135                 140                 


Tyr Pro Asn Leu Ile Thr Asp Asp Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Gly Leu Thr Ser Lys Ile Ser Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Glu Glu Asn Lys Asp Lys Leu Val Glu 
        195                 200                 205             


Phe Tyr Ala Leu Lys Val Val Ala Val Glu Asp Ser Gly Glu Lys Lys 
    210                 215                 220                 


Gly Lys Asp Ile Trp Tyr Asn Val His Leu Glu Asn Gly Trp Val Tyr 
225                 230                 235                 240 


Arg Arg Thr Ser Asn Met Ser Leu Asp Trp Val Gly Lys Thr Lys Glu 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Glu Lys Asp Gly Thr Pro Lys Lys Asp 
            260                 265                 270         


Lys Glu Gly Asn Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp 
        275                 280                 285             


Trp Ser Leu Val Lys Lys Lys Thr Glu Ala Asp Ile Asp Gln Thr His 
    290                 295                 300                 


Lys Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Gly Asn Pro Ser 
305                 310                 315                 320 


Gln Lys Ile Arg Gly Arg Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Glu Glu Leu Lys Leu Ile Leu Glu Lys Gln Lys Glu Phe His Leu 
            340                 345                 350         


Glu Leu Gln Asp Arg Ala Leu Tyr Ala Ala Cys Ile Glu Glu Leu Tyr 
        355                 360                 365             


Pro Ile Asn Glu Ala His Arg Asn Asn Val Gly Asn Arg Asp Phe Thr 
    370                 375                 380                 


Tyr Leu Phe Leu Glu Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys Ser 
385                 390                 395                 400 


Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu Asn Gln Gly Leu 
                405                 410                 415     


Asp Lys Glu Thr Gly Glu Ile Lys Ser Phe Pro Val Lys Cys Ile Ala 
            420                 425                 430         


Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Ile Ile 
        435                 440                 445             


Asn Leu Arg Ile Tyr Arg Lys Asp Leu Ala Gln Asp Val Asp Val Thr 
    450                 455                 460                 


Asn Glu Leu Leu Lys Thr Glu Glu Asp Tyr Val Ala Leu Phe Asp Trp 
465                 470                 475                 480 


Leu Asn Gly Lys Lys Glu Ile Asp Gln Lys Ala Phe Leu Lys Tyr Pro 
                485                 490                 495     


Val Phe Gly Leu Lys Lys Glu Ile Glu Asn Tyr Arg Trp Asn Tyr Val 
            500                 505                 510         


Gln Asp Lys Pro Tyr Pro Cys Asn Glu Thr Arg Ser Leu Met Leu Ala 
        515                 520                 525             


Arg Leu Glu Lys Cys Asp Ile Ser Ile Glu Phe Leu Thr Lys Glu Asn 
    530                 535                 540                 


Glu Glu Ala Leu Trp His Ile Leu Tyr Ser Val Glu Asp Lys Asn Glu 
545                 550                 555                 560 


Ile Val Lys Ala Leu Gln Lys Phe Ala Asp Lys His Asp Leu Asn Ala 
                565                 570                 575     


Ser Phe Val Asp Val Phe Gln Lys Phe Pro Pro Phe Lys Lys Glu Tyr 
            580                 585                 590         


Gly Ser Tyr Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg 
        595                 600                 605             


Met Gly Lys Tyr Trp Ser Glu Asn Ser Ile Asp Lys Gly Thr Arg Glu 
    610                 615                 620                 


Arg Ile Asp Lys Ile Ile Thr Gly Glu Tyr Asp Glu Lys Ile Glu Asn 
625                 630                 635                 640 


Arg Val Arg Glu Lys Ala Ile His Leu Ala Asp Val Ser Asp Phe Lys 
                645                 650                 655     


Gly Leu Pro Leu Trp Leu Val Cys Tyr Ile Val Tyr Gly Arg His Ser 
            660                 665                 670         


Glu Ala Lys Glu Ile Ile Gln Trp Lys Ser Pro Ala Asp Ile Asp Ser 
        675                 680                 685             


Tyr Leu Arg Ser Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu 
    690                 695                 700                 


Gln Ile Ile Ile Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Gln 
705                 710                 715                 720 


Val Gly Asn Ile Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys 
                725                 730                 735     


Asn Pro Ala Asp Lys Arg Lys Lys Met Thr Gln Gln Met Ser Glu Asn 
            740                 745                 750         


Glu Asn Thr Asn Leu Arg Ile Lys Tyr Leu Leu Thr Glu Phe Leu Asn 
        755                 760                 765             


Pro Glu Tyr Glu Val Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln 
    770                 775                 780                 


Asp Ile Leu Arg Ile Tyr Glu Glu Gly Val Leu Asn Ser Val Ser Asp 
785                 790                 795                 800 


Leu Pro Glu Glu Ile Ser Asp Ile Leu Lys Lys Phe Thr Glu Thr Asp 
                805                 810                 815     


Leu Lys Lys Arg Pro Ser Arg Ser Glu Val Leu Arg Tyr Lys Leu Trp 
            820                 825                 830         


Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Glu Val Ile Pro Leu 
        835                 840                 845             


Gly Lys Leu Phe Thr Pro Ala Tyr Glu Ile Glu His Val Ile Pro Gln 
    850                 855                 860                 


Ser Tyr Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val Ile Cys Glu Ala 
865                 870                 875                 880 


Glu Val Asn Lys Leu Lys Ser Asn Leu Leu Gly His Glu Phe Ile Val 
                885                 890                 895     


Lys His His Gly Glu Ile Val Glu Leu Pro Phe Gly Lys Lys Val Arg 
            900                 905                 910         


Ile Phe Thr Val Asp Glu Tyr Glu Gln Phe Val Lys Asp Asn Tyr Ser 
        915                 920                 925             


Arg Thr Arg Ser Lys Met Lys Lys Leu Leu Met Asp Asp Ile Pro Glu 
    930                 935                 940                 


Glu Phe Val Thr Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val 
945                 950                 955                 960 


Val Lys Ser Leu Leu Ser Asn Ile Val Arg Glu Lys Gly Glu Glu Glu 
                965                 970                 975     


Ala Ile Ser Lys Asn Val Ile Pro Cys Thr Gly Gly Val Thr Asp Arg 
            980                 985                 990         


Leu Lys Lys Asp Trp Gly Ile Asn  Glu Val Trp Asn Lys  Ile Ile Leu 
        995                 1000                 1005             


Pro Arg  Phe Gln Arg Leu Asn  Glu Leu Thr Gly Thr  Asn Lys Phe 
    1010                 1015                 1020             


Thr Thr  Lys Asn Val Gly Ile  Gln Glu Ile Pro Thr  Met Pro Leu 
    1025                 1030                 1035             


Glu Leu  Gln Lys Gly Phe Asn  Lys Lys Arg Ile Asp  His Arg His 
    1040                 1045                 1050             


His Ala  Met Asp Ala Ile Ile  Ile Ala Cys Ala Asn  Arg Asn Ile 
    1055                 1060                 1065             


Ile Asn  Tyr Leu Asn Asn Lys  Ser Ala Thr Ala Lys  Ala Glu Leu 
    1070                 1075                 1080             


Ser Arg  Tyr Asp Leu Gln Lys  Met Leu Cys Asp Lys  Ala Lys Thr 
    1085                 1090                 1095             


Asp Asn  Asn Gly Asn Tyr Lys  Trp Val Ile Arg Lys  Pro Trp Ala 
    1100                 1105                 1110             


Ser Phe  Thr Gln Asp Thr Tyr  Leu Ala Leu Glu Asn  Ile Ile Val 
    1115                 1120                 1125             


Ser Phe  Lys Gln Asn Leu Arg  Val Ile Asn Lys Ala  Thr Asn Arg 
    1130                 1135                 1140             


Phe Leu  His Tyr Asn Glu Glu  Gly Lys Lys Ile Phe  Val Lys Gln 
    1145                 1150                 1155             


Gly Lys  Gly Asp Asn Trp Ala  Ile Arg Lys Ser Met  His Lys Asp 
    1160                 1165                 1170             


Thr Val  Phe Gly Glu Val Asn  Leu Arg Arg Ile Lys  Thr Val Ala 
    1175                 1180                 1185             


Leu Asn  Glu Ala Met Lys Asn  Pro Gln Ser Ile Val  Val Lys Asp 
    1190                 1195                 1200             


Phe Lys  Arg Lys Leu Leu Glu  Leu Trp Asn Leu Gly  Phe Asp Ala 
    1205                 1210                 1215             


Lys Arg  Ile Lys Lys Tyr Phe  Glu Asp Asn Arg Glu  Thr Trp Ser 
    1220                 1225                 1230             


Asp Ile  Asn Leu Ser Lys Ile  Glu Val Tyr Tyr Phe  Ser Lys Asp 
    1235                 1240                 1245             


Thr Lys  Asp Arg Phe Phe Ala  Thr Arg Lys Pro Leu  Asp Thr Ser 
    1250                 1255                 1260             


Phe Asp  Arg Lys Lys Ile Glu  Asn Asn Ile Thr Asp  Thr Gly Ile 
    1265                 1270                 1275             


Gln Lys  Ile Leu Leu Arg His  Leu Glu Leu Lys Asp  Asn Asn Pro 
    1280                 1285                 1290             


Asp Ile  Ala Phe Ser Pro Asp  Gly Ile Asp Glu Met  Asn Arg Asn 
    1295                 1300                 1305             


Ile Ile  Gln Leu Asn Asn Gly  Lys Tyr His Gln Pro  Ile Ile Lys 
    1310                 1315                 1320             


Ile Arg  Trp Tyr Glu Gln Ala  Asp Lys Phe Ala Val  Gly Gln Thr 
    1325                 1330                 1335             


Gly Asn  Lys Ser Ser Lys Phe  Val Glu Ala Ala Lys  Gly Thr Asn 
    1340                 1345                 1350             


Leu Phe  Phe Ala Val Tyr Glu  Ser Asn Ile Leu Asp  Lys Lys Thr 
    1355                 1360                 1365             


Asn Thr  Ile Ile Lys Lys Arg  Asn Tyr Ala Thr Ile  Pro Leu Asn 
    1370                 1375                 1380             


Val Ala  Ile Glu Arg Gln Lys  Gln Gly Leu Pro Val  Ala Pro Glu 
    1385                 1390                 1395             


Asp Glu  Asn Gly Asn Asp Pro  Ile Phe Val Leu Ser  Pro Asn Asp 
    1400                 1405                 1410             


Leu Val  Tyr Leu Pro Thr Asp  Asp Glu Leu Ala Asn  Gly Ile Ile 
    1415                 1420                 1425             


Ala Gln  Pro Leu Asp Arg Gly  Arg Ile Tyr Lys Met  Val Ser Ser 
    1430                 1435                 1440             


Ser Gly  Glu Gln Cys Phe Phe  Ile Lys His Ile Val  Ala Asn Val 
    1445                 1450                 1455             


Leu Val  Asp Lys Phe Glu Phe  Ser Pro Leu Asn Lys  Met Glu Arg 
    1460                 1465                 1470             


Ala Leu  Thr Gly Glu Met Ile  Lys Met Ile Cys Ile  Pro Ile Lys 
    1475                 1480                 1485             


Val Asp  Arg Leu Gly Asn Val  Leu Glu Ser Ser Ser  Ser Tyr Lys 
    1490                 1495                 1500             


Lys 
    


<210>  1308
<211>  1504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1308

Met Lys Asn Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Gly Ser Val Asn Asp Asp Gly Ser Glu Gln Leu Val 
            20                  25                  30          


Lys Ile Gln Ala Ser Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Met 
        35                  40                  45              


Ile Gly Asp Phe Asn Lys Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg 
    50                  55                  60                  


Thr Arg Leu Arg Gly Val Arg Arg Leu Ser Glu Arg Tyr Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Ile Leu Asp Ile Leu Gly Phe Leu Pro Phe 
                85                  90                  95      


His Phe Ala Gln Asp Leu Asp Arg His Gly Lys Ile Val Lys Gly Lys 
            100                 105                 110         


Glu Pro Lys Leu Ala Trp Arg Lys Asn Glu Ala Gly Gln Phe Glu Phe 
        115                 120                 125             


Ile Phe Gln Asp Ser Phe Lys Glu Met Leu Glu Asp Phe Lys Leu Asn 
    130                 135                 140                 


His Pro Asn Leu Ile Thr Asp Asp Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Gly Leu Thr Ser Lys Ile Ser Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Glu Glu Asn Lys Asn Lys Leu Val Glu 
        195                 200                 205             


Phe Tyr Ala Leu Lys Val Val Ala Val Glu Asp Ser Gly Glu Lys Lys 
    210                 215                 220                 


Gly Lys Asp Ile Trp Tyr Asn Val His Leu Glu Asn Gly Trp Val Tyr 
225                 230                 235                 240 


Arg Arg Thr Ser Asn Met Pro Leu Asp Trp Val Gly Lys Thr Lys Glu 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Glu Lys Asp Gly Thr Pro Lys Lys Asp 
            260                 265                 270         


Lys Glu Gly Asn Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp 
        275                 280                 285             


Trp Ser Leu Leu Lys Lys Lys Thr Glu Ala Asp Ile Asp Gln Thr His 
    290                 295                 300                 


Lys Thr Val Gly Val Tyr Ile Tyr Asp Thr Leu Leu Gly Asn Pro Ser 
305                 310                 315                 320 


Gln Lys Ile Arg Gly Arg Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Glu Glu Leu Lys Gln Ile Leu Glu Lys Gln Lys Glu Phe His Leu 
            340                 345                 350         


Glu Leu Gln Asp Arg Ala Leu Tyr Ala Ala Cys Ile Glu Glu Leu Tyr 
        355                 360                 365             


Pro Ile Asn Glu Ala His Arg Asn Asn Val Gly Asn Arg Asp Phe Thr 
    370                 375                 380                 


Tyr Leu Phe Leu Glu Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys Ser 
385                 390                 395                 400 


Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu Asn Gln Gly Leu 
                405                 410                 415     


Asp Lys Glu Thr Gly Glu Ile Lys Ser Phe Pro Val Lys Cys Ile Ala 
            420                 425                 430         


Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Ile Val 
        435                 440                 445             


Asn Leu Arg Ile Tyr Arg Lys Asp Leu Ala Gln Asp Val Asp Val Thr 
    450                 455                 460                 


Asn Glu Leu Leu Lys Thr Glu Glu Asp Tyr Val Ala Leu Phe Asp Trp 
465                 470                 475                 480 


Leu Asn Gly Lys Lys Glu Ile Asp Gln Lys Ala Phe Leu Lys Tyr Pro 
                485                 490                 495     


Val Phe Gly Leu Lys Lys Glu Ile Glu Asn Tyr Arg Trp Asn Tyr Val 
            500                 505                 510         


Gln Asp Lys Pro Tyr Pro Cys Asn Glu Thr Arg Ser Leu Met Leu Ala 
        515                 520                 525             


Arg Leu Glu Lys Cys Asp Ile Ser Ile Glu Phe Leu Thr Lys Glu Asn 
    530                 535                 540                 


Glu Glu Ala Leu Trp His Ile Leu Tyr Ser Val Glu Asp Lys Asn Glu 
545                 550                 555                 560 


Ile Val Lys Ala Leu Gln Lys Phe Ala Asp Lys His Asp Leu Asn Ala 
                565                 570                 575     


Ser Phe Val Asp Val Phe Gln Lys Phe Pro Pro Phe Lys Lys Glu Tyr 
            580                 585                 590         


Gly Ser Tyr Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg 
        595                 600                 605             


Met Gly Lys Tyr Trp Ser Glu Asn Ser Ile Asp Lys Gly Thr Arg Glu 
    610                 615                 620                 


Arg Ile Asp Lys Ile Ile Thr Gly Glu Tyr Asp Glu Lys Ile Glu Asn 
625                 630                 635                 640 


Arg Val Arg Glu Lys Ala Ile His Leu Ala Asp Val Ser Asp Phe Lys 
                645                 650                 655     


Gly Leu Pro Leu Trp Leu Val Cys Tyr Ile Val Tyr Gly Arg His Ser 
            660                 665                 670         


Glu Ala Lys Glu Ile Thr Gln Trp Lys Ser Pro Ala Asp Ile Asp Ser 
        675                 680                 685             


Tyr Leu Arg Ser Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu 
    690                 695                 700                 


Gln Ile Ile Ile Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Gln 
705                 710                 715                 720 


Val Gly Asn Ile Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys 
                725                 730                 735     


Asn Pro Ala Asp Lys Arg Lys Lys Met Thr Gln Gln Met Ser Glu Asn 
            740                 745                 750         


Glu Asn Thr Asn Leu Arg Ile Lys Tyr Leu Leu Thr Glu Phe Leu Asn 
        755                 760                 765             


Pro Glu Tyr Glu Val Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln 
    770                 775                 780                 


Asp Ile Leu Arg Ile Tyr Glu Glu Gly Val Leu Asn Ser Val Ser Asp 
785                 790                 795                 800 


Leu Pro Glu Glu Ile Ser Asp Ile Leu Lys Lys Phe Thr Glu Thr Asp 
                805                 810                 815     


Leu Lys Lys Arg Pro Ser Arg Ser Glu Ile Leu Arg Tyr Lys Leu Trp 
            820                 825                 830         


Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Glu Val Ile Pro Leu 
        835                 840                 845             


Gly Lys Leu Phe Thr Pro Ala Tyr Glu Ile Glu His Val Ile Pro Gln 
    850                 855                 860                 


Ser Cys Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val Ile Cys Glu Ala 
865                 870                 875                 880 


Glu Val Asn Lys Leu Lys Ser Asn Leu Leu Gly His Glu Phe Ile Val 
                885                 890                 895     


Lys His His Gly Glu Ile Val Glu Leu Pro Phe Gly Lys Lys Val Arg 
            900                 905                 910         


Ile Phe Thr Val Asp Glu Tyr Glu Gln Phe Val Lys Asp Asn Tyr Ser 
        915                 920                 925             


Arg Thr Arg Ser Lys Met Lys Lys Leu Leu Met Asp Asp Ile Pro Glu 
    930                 935                 940                 


Glu Phe Val Thr Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val 
945                 950                 955                 960 


Val Lys Ser Leu Leu Ser Asn Ile Val Arg Glu Lys Gly Glu Glu Glu 
                965                 970                 975     


Ala Ile Ser Lys Asn Val Ile Pro Cys Thr Gly Gly Val Thr Asp Arg 
            980                 985                 990         


Leu Lys Lys Asp Trp Gly Ile Asn  Glu Val Trp Asn Lys  Ile Ile Leu 
        995                 1000                 1005             


Pro Arg  Phe Gln Arg Leu Asn  Glu Leu Thr Gly Thr  Asn Lys Phe 
    1010                 1015                 1020             


Thr Thr  Lys Asn Val Gly Ile  Gln Glu Ile Pro Thr  Met Pro Leu 
    1025                 1030                 1035             


Glu Leu  Gln Lys Gly Phe Asn  Lys Lys Arg Ile Asp  His Arg His 
    1040                 1045                 1050             


His Ala  Met Asp Ala Ile Ile  Ile Ala Cys Ala Asn  Arg Asn Ile 
    1055                 1060                 1065             


Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Thr Ala Lys  Ala Glu Leu 
    1070                 1075                 1080             


Ser Arg  Tyr Asp Leu Gln Lys  Met Leu Cys Asp Lys  Ala Lys Thr 
    1085                 1090                 1095             


Asp Asn  Asn Gly Asn Tyr Lys  Trp Val Ile Arg Lys  Pro Trp Ala 
    1100                 1105                 1110             


Ser Phe  Thr Gln Asp Thr Tyr  Leu Ala Leu Glu Asn  Ile Ile Val 
    1115                 1120                 1125             


Ser Phe  Lys Gln Asn Leu Arg  Val Ile Asn Lys Ala  Thr Asn Arg 
    1130                 1135                 1140             


Phe Leu  His Tyr Asn Glu Glu  Gly Lys Lys Ile Phe  Val Lys Gln 
    1145                 1150                 1155             


Gly Lys  Gly Asp Asn Trp Ala  Ile Arg Lys Ser Met  His Lys Asp 
    1160                 1165                 1170             


Thr Val  Phe Gly Glu Val Asn  Leu Arg Arg Ile Lys  Thr Val Ala 
    1175                 1180                 1185             


Leu Asn  Glu Ala Met Lys Asn  Pro Gln Ser Ile Val  Val Lys Asp 
    1190                 1195                 1200             


Phe Lys  Arg Lys Leu Leu Glu  Leu Trp Asn Leu Gly  Phe Asp Ala 
    1205                 1210                 1215             


Lys Arg  Ile Lys Lys Tyr Phe  Glu Asp Asn Arg Glu  Thr Trp Ser 
    1220                 1225                 1230             


Asp Ile  Asn Leu Ser Lys Ile  Glu Val Tyr Tyr Phe  Ser Lys Asp 
    1235                 1240                 1245             


Thr Lys  Asp Arg Phe Phe Ala  Thr Arg Lys Pro Leu  Asp Thr Ser 
    1250                 1255                 1260             


Phe Asp  Arg Lys Lys Ile Glu  Asn Asn Ile Thr Asp  Thr Gly Ile 
    1265                 1270                 1275             


Gln Lys  Ile Leu Leu Arg His  Leu Glu Leu Lys Asp  Asn Asn Pro 
    1280                 1285                 1290             


Asp Ile  Ala Phe Ser Pro Asp  Gly Ile Asp Glu Met  Asn Arg Asn 
    1295                 1300                 1305             


Ile Ile  Gln Leu Asn Asn Gly  Lys Tyr His Gln Pro  Ile Ile Lys 
    1310                 1315                 1320             


Ile Arg  Trp Tyr Glu Gln Ala  Asp Lys Phe Ala Val  Gly Gln Thr 
    1325                 1330                 1335             


Gly Asn  Lys Phe Ser Lys Phe  Val Glu Ala Ala Lys  Gly Thr Asn 
    1340                 1345                 1350             


Leu Phe  Phe Ala Val Tyr Glu  Ser Asn Ile Leu Asp  Lys Lys Thr 
    1355                 1360                 1365             


Asn Thr  Ile Ile Lys Lys Arg  Asn Tyr Ala Thr Ile  Pro Leu Asn 
    1370                 1375                 1380             


Val Ala  Ile Glu Arg Gln Lys  Gln Gly Leu Pro Val  Ala Pro Glu 
    1385                 1390                 1395             


Asp Glu  Asn Gly Asn Asp Pro  Ile Phe Val Leu Ser  Pro Asn Asp 
    1400                 1405                 1410             


Leu Val  Tyr Leu Pro Thr Asp  Asp Glu Leu Ala Asn  Gly Ile Ile 
    1415                 1420                 1425             


Ala Gln  Pro Leu Asp Arg Gly  Arg Ile Tyr Lys Met  Val Ser Cys 
    1430                 1435                 1440             


Thr Gly  Asn Glu Gly His Phe  Ile Pro Ala Arg Ile  Ala Asn Pro 
    1445                 1450                 1455             


Ile Leu  Gln Thr Ile Glu Leu  Gly Ser Asn Asn Lys  Ala Gln Lys 
    1460                 1465                 1470             


Ala Trp  Thr Asp Glu Met Ile  Lys Glu Ile Cys Met  Pro Ile Lys 
    1475                 1480                 1485             


Val Asp  Arg Leu Gly Asn Val  Leu Glu Ser Ser Ser  Ser Tyr Lys 
    1490                 1495                 1500             


Lys 
    


<210>  1309
<211>  1506
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1309

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Pro Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Lys Val Glu Val Asp Asp Glu Gly Asn Tyr Leu Arg Asn Ile Lys 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Thr Gln Asp Met Leu Gly Asp Phe 
        35                  40                  45              


Asp Lys Gly Val Lys Val Ser Gln Thr Ala Ile Arg Thr Asp Phe Arg 
    50                  55                  60                  


Gly Asn Arg Arg Leu Ile Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Ala Leu Lys Phe Leu Pro Lys His Tyr Glu Asp 
                85                  90                  95      


Ala Ile Gly Trp Asp Arg Asn Glu Pro Ala Thr Tyr Gly Lys Phe Val 
            100                 105                 110         


Asn Asn Ser Glu Pro Lys Leu Ala Trp Glu Arg Gln Glu Asp Gly Ser 
        115                 120                 125             


Met Arg Phe Leu Phe Met Glu Ser Phe Asn Glu Met Val Ala Asp Phe 
    130                 135                 140                 


Ala Gln Asn Gln Pro Ser Leu Val Ser Asp Gly Lys Lys Ile Pro Leu 
145                 150                 155                 160 


Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Arg Pro Ile 
                165                 170                 175     


Lys Lys Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg 
            180                 185                 190         


Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Asp Asn Leu Thr Lys 
        195                 200                 205             


Arg Lys Glu Tyr Tyr Lys Leu Lys Val Val Gly Val Glu Ala Asp Gly 
    210                 215                 220                 


Ala Pro Lys Gly Gly Lys Thr Trp Tyr Asn Ile His Leu Glu Asn Gly 
225                 230                 235                 240 


Trp Thr Ala Arg Cys Gln Ser Lys Ile Pro Leu Asp Asp Trp Val Gly 
                245                 250                 255     


Lys Val Lys Glu Tyr Ile Val Thr Thr Glu Tyr Glu Asp Asp Gly Lys 
            260                 265                 270         


Thr Pro Lys Cys Asp Lys Glu Gly Lys Val Lys Arg Ser Phe Lys Ser 
        275                 280                 285             


Pro Glu Lys Asp Asp Trp Gln Leu Ile Lys Thr Arg Thr Glu Lys Phe 
    290                 295                 300                 


Ile Asp Asp Ser Asn Lys Thr Val Gly Ala Phe Ile Tyr Asp Tyr Leu 
305                 310                 315                 320 


Leu Glu Lys Pro Phe Asp Lys Ile Arg Gly Lys Tyr Ile Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Asn Glu Leu Arg Glu Ile Leu Arg Glu Gln 
            340                 345                 350         


Thr Lys Tyr His Glu Gly Leu His Ser Leu Glu Ala Leu Lys Ile Cys 
        355                 360                 365             


Ala Glu Glu Leu Tyr Lys Asn Asn Gln Gln His Gln Glu Tyr Leu Leu 
    370                 375                 380                 


Lys Lys Asp Met Thr Phe Leu Leu Leu Asn Asp Leu Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asp Cys Pro Tyr Glu 
                405                 410                 415     


Val Tyr Lys Tyr Asp Asp Lys Leu Thr Gly Lys Lys Gly Gln Lys Lys 
            420                 425                 430         


Ile Lys Cys Ile Ala Lys Ser Asn Pro Tyr Tyr Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Ile Ala Asn Leu Arg Leu Phe Ser Val Thr Asp Gly Lys 
    450                 455                 460                 


Glu Val Thr Ala Glu Tyr Leu Lys Thr Glu Glu Asp Tyr Val Arg Leu 
465                 470                 475                 480 


Phe Ser Phe Leu Asn Asp Arg Lys Asp Ile Lys Gln Glu Thr Phe Leu 
                485                 490                 495     


Arg Ser Phe Leu Lys Leu Lys Lys Glu Ser Ile Gly Ser Glu Lys Ile 
            500                 505                 510         


Tyr Pro Ile Arg Trp Asn Tyr Ile Glu Asp Ala Asn Lys Ser Tyr Pro 
        515                 520                 525             


Cys Asn Ala Thr Arg His Glu Leu Leu Leu Ala Phe Asp Arg Ala Asp 
    530                 535                 540                 


Ile Pro Arg Glu Leu Leu Asp Val Glu Glu Arg Glu Tyr Arg Leu Trp 
545                 550                 555                 560 


His Leu Leu Tyr Ser Ile Glu Ala Lys Glu Glu Thr Glu Ser Ala Leu 
                565                 570                 575     


Arg Lys Leu Lys Ala Asn Lys Ala Phe Gly Asp Leu Asn Ile Asp Asp 
            580                 585                 590         


Ala Phe Val Asn Ser Phe Leu Lys Ile Lys Pro Phe Glu Lys Glu Tyr 
        595                 600                 605             


Gly Ala Tyr Ser Glu Lys Ala Ile Lys Lys Leu Leu Ala Val Met Arg 
    610                 615                 620                 


Arg Gly Ser Leu Trp Lys Glu Ala Asp Ile Cys Glu Ala Thr Lys Gln 
625                 630                 635                 640 


Asn Ile Ala Lys Ile Leu Gln Asn Asp Ile Asp Glu Lys Leu Lys Lys 
                645                 650                 655     


Lys Ile Glu Ala Ala Val Ser Ser Phe Asn Lys Leu Ser Asp Phe Gln 
            660                 665                 670         


Gly Leu Pro Thr Trp Leu Ala Cys Tyr Val Val Tyr Gly Arg His Ser 
        675                 680                 685             


Glu Ala Ala Gln Ile Gln Cys Trp Gln Ser Pro Glu Asp Leu Lys Thr 
    690                 695                 700                 


Tyr Ile Asn Lys Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu 
705                 710                 715                 720 


Gln Cys Leu Leu Glu Thr Leu Arg Thr Val Tyr Asp Ile Trp Lys Asp 
                725                 730                 735     


Ala Gly Gln Ile Asp Glu Ile His Ile Glu Leu Gly Arg Asn Met Lys 
            740                 745                 750         


Ser Thr Ala Glu Gln Arg Ala Arg Lys Thr Lys Asp Ile Leu Arg Asn 
        755                 760                 765             


Glu Asn Thr Asn Leu Arg Ile Lys Asn Leu Leu Val Glu Leu Lys Asn 
    770                 775                 780                 


Asp Asp Lys Ile Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln Glu 
785                 790                 795                 800 


Ile Leu Arg Ile Tyr Glu Glu Gly Ala Leu Gln Glu Leu Ser Lys Glu 
                805                 810                 815     


Asp Glu Met Phe Glu Asp Ile Ser Lys Ile Ser Lys Met Ala Gln Pro 
            820                 825                 830         


Ser Pro Thr Glu Leu Ser Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr 
        835                 840                 845             


Arg Ser Pro Tyr Thr Gly Arg Val Ile Pro Leu Ser Lys Leu Phe Thr 
    850                 855                 860                 


Thr Ala Tyr Gln Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Phe Asp 
865                 870                 875                 880 


Asp Ser Phe Asn Asn Lys Val Ile Cys Glu Ser Glu Val Asn Ser Leu 
                885                 890                 895     


Lys Asp Asn Met Leu Gly Tyr Glu Phe Ile Lys Glu Cys Gly Gly Arg 
            900                 905                 910         


Ile Val His Cys Thr Leu Leu Gly Asp Val Lys Ile Phe Thr Lys Glu 
        915                 920                 925             


Glu Tyr Lys Asp Phe Val Thr Lys His Tyr Ile Asp Asn Thr Tyr Lys 
    930                 935                 940                 


Arg Asp Lys Leu Leu Leu Glu Asp Ile Pro Gln Glu Phe Ser Ala Arg 
945                 950                 955                 960 


Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Met Val Gln Thr Val Leu 
                965                 970                 975     


Ser Asn Ile Val Arg Ala Glu Asn Glu Gln Glu Ala Thr Ser Lys Phe 
            980                 985                 990         


Val Val Ser Cys Asn Gly Ala Ile  Thr Asn Arg Leu Lys  Lys Asp Trp 
        995                 1000                 1005             


Gly Leu  Asn Asp Val Trp Ser  Ser Ile Val Cys Pro  Arg Phe Lys 
    1010                 1015                 1020             


Arg Leu  Asn Glu Ile Thr Lys  Thr Asn Ala Phe Gly  His Trp Glu 
    1025                 1030                 1035             


Asp Lys  Ala Gly Lys His Val  Phe Gln Thr Thr Val  Pro Leu Glu 
    1040                 1045                 1050             


Leu Gln  Arg Gly Phe Ser Lys  Lys Arg Ile Asp His  Arg His His 
    1055                 1060                 1065             


Ala Met  Asp Ala Leu Val Ile  Ala Cys Ala Ser Arg  Asn Ile Val 
    1070                 1075                 1080             


Asn Tyr  Leu Asn Asn Glu Ser  Ala Asn Ser Pro Glu  Lys Arg Glu 
    1085                 1090                 1095             


Asp Leu  Arg Lys Lys Leu Cys  Asp Glu Lys Arg Lys  Ile Arg Lys 
    1100                 1105                 1110             


Pro Trp  Lys Thr Phe Thr Gln  Asp Ala Arg Glu Ala  Leu Ser Asp 
    1115                 1120                 1125             


Val Val  Val Ser Phe Lys Asn  Tyr Val Arg Val Ile  Asn Lys Ala 
    1130                 1135                 1140             


Thr Asn  Tyr Tyr Glu His Tyr  Ser Ser Ser Gly Asn  Lys Val Met 
    1145                 1150                 1155             


Ile Ser  Gln Lys Gly Asn Asp  Met Trp Ala Ile Arg  Lys Pro Leu 
    1160                 1165                 1170             


His Lys  Glu Ser Val Phe Gly  Arg Val Asn Leu Cys  Arg Lys Val 
    1175                 1180                 1185             


Ile Val  Ser Leu Lys Lys Ala  Leu Glu Asn Ile Pro  Ala Ile Cys 
    1190                 1195                 1200             


Asp Lys  Thr Leu Arg Asp Tyr  Ile Asn Ala Leu Val  Glu Lys His 
    1205                 1210                 1215             


Phe Asn  Thr Arg Gln Leu Leu  Ala His Phe Lys Ser  Ile Asn Tyr 
    1220                 1225                 1230             


Arg Leu  Asn Arg Gln Cys Val  Glu Lys Val Glu Val  Trp Gln Phe 
    1235                 1240                 1245             


Ser Asp  Glu Lys Ile Glu Met  Val Ala Arg Arg Lys  Ser Leu Asp 
    1250                 1255                 1260             


Thr Ser  Phe Asp Asp Lys Gln  Ile Ser Ser Ile Thr  Asp Thr Gly 
    1265                 1270                 1275             


Ile Gln  Lys Ile Leu Leu Asn  Tyr Leu Gln Thr Lys  Asp Gly Asn 
    1280                 1285                 1290             


Ala Glu  Ile Ala Phe Thr Pro  Glu Gly Ile Glu Glu  Met Asn Lys 
    1295                 1300                 1305             


Asn Ile  Ser Leu Tyr Asn Asp  Gly Lys Ala His Gln  Pro Ile Leu 
    1310                 1315                 1320             


Lys Val  Arg Val Ala Thr Pro  Lys Gly Met Lys Tyr  Pro Val Gly 
    1325                 1330                 1335             


Gln Thr  Gly Asn Lys Ala Thr  Lys Phe Val Val Ala  Glu Lys Gly 
    1340                 1345                 1350             


Thr Asn  Leu Tyr Phe Ala Ile  Tyr Glu Asn Glu Leu  Gly Val Arg 
    1355                 1360                 1365             


Ser Tyr  Asp Thr Ile Pro Leu  Asn Ile Val Val Glu  Arg Leu Lys 
    1370                 1375                 1380             


Glu Gly  Leu Ser Pro Val Pro  Gln Ala Asn Glu Ala  Asp Ile Pro 
    1385                 1390                 1395             


Leu Lys  Phe Tyr Leu Ser Pro  Asn Asp Leu Val Tyr  Val Pro Thr 
    1400                 1405                 1410             


Lys Glu  Asn Glu Asp Glu Leu  Cys Arg Asp Arg Ile  Tyr Lys Phe 
    1415                 1420                 1425             


Val Asp  Ser Ser Gly Thr Thr  Ala Asn Phe Val Pro  His Thr Ser 
    1430                 1435                 1440             


Ala Asn  Thr Ile Tyr Asn Val  Asp Lys Glu Tyr Ala  Glu Lys Cys 
    1445                 1450                 1455             


Cys Asn  Gly Ala Val Phe Leu  Asn Glu Tyr Gly Leu  Gly Ser Pro 
    1460                 1465                 1470             


Gln Ser  Lys Asn Gln Arg Ala  Leu Thr Gly Glu Met  Ile Lys Glu 
    1475                 1480                 1485             


Ile Cys  Trp Lys Leu Lys Val  Asp Arg Leu Gly Asn  Ile Ile Lys 
    1490                 1495                 1500             


Val Ile  Lys 
    1505     


<210>  1310
<211>  1509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1310

Met Lys Asn Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Gly Ser Val Asn Asp Asp Gly Ser Glu Gln Leu Val 
            20                  25                  30          


Lys Ile Gln Ala Ser Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Met 
        35                  40                  45              


Ile Gly Asp Phe Asn Lys Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg 
    50                  55                  60                  


Thr Arg Leu Arg Gly Val Arg Arg Leu Ser Glu Arg Tyr Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Ile Leu Asp Ile Leu Gly Phe Leu Pro Phe 
                85                  90                  95      


His Phe Ala Gln Asp Leu Asp Arg His Gly Lys Ile Val Lys Gly Lys 
            100                 105                 110         


Glu Pro Lys Leu Ala Trp Arg Lys Asn Glu Ala Gly Gln Phe Glu Phe 
        115                 120                 125             


Ile Phe Gln Asp Ser Phe Lys Glu Met Leu Glu Asp Phe Lys Leu Asn 
    130                 135                 140                 


His Pro Asn Leu Ile Thr Asp Asp Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Gly Leu Thr Ser Lys Ile Ser Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Lys Lys Asn Asp Asn Val Met Ile Ile 
        195                 200                 205             


Asn Ser Lys Val Ile Asp Val Val Lys Ala Glu Lys Asp Lys Lys Tyr 
    210                 215                 220                 


Glu Lys Tyr Trp Tyr Asn Val Leu Leu Glu Asn Gly Leu Leu Tyr Arg 
225                 230                 235                 240 


Ala Ala Phe Tyr Asn Asp Ile Ser Phe Trp Lys Gly His Thr Lys Glu 
                245                 250                 255     


Phe Val Leu Lys Thr Ile Lys Leu Lys Ser Gly Gly Thr Lys Gln Glu 
            260                 265                 270         


Leu Ser Phe Leu Pro Ser Phe Asp Glu Ile Glu Lys Met Glu Lys Lys 
        275                 280                 285             


Gln Lys Asp Lys Met Tyr Phe Lys Ile Lys Leu Lys Thr Glu Ala Asp 
    290                 295                 300                 


Ile Asp Gln Ala His Lys Thr Val Gly Thr Tyr Ile Tyr Asp Thr Leu 
305                 310                 315                 320 


Leu Gly Asn Pro Ser Gln Lys Ile Arg Gly Arg Leu Val Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Lys Glu Glu Leu Lys Leu Ile Leu Asp Lys Gln 
            340                 345                 350         


Lys Glu Phe His Pro Glu Leu Gln Asp Arg Ala Leu Tyr Ala Ala Cys 
        355                 360                 365             


Ile Glu Glu Leu Tyr Pro Ile Asn Glu Ala His Arg Asn Asn Val Gly 
    370                 375                 380                 


Asn Arg Asp Phe Thr Tyr Leu Phe Leu Glu Asp Ile Leu Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu 
                405                 410                 415     


Glu Asn Gln Gly Leu Asp Lys Glu Thr Gly Glu Ile Lys Ser Phe Pro 
            420                 425                 430         


Val Lys Cys Ile Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Ile Val Asn Leu Arg Ile Tyr Arg Lys Asp Leu Ala Gln 
    450                 455                 460                 


Asp Val Asp Val Thr Asn Glu Leu Leu Lys Thr Glu Glu Asp Tyr Val 
465                 470                 475                 480 


Ala Leu Phe Asp Trp Leu Asn Gly Lys Lys Glu Ile Asp Gln Lys Ala 
                485                 490                 495     


Phe Leu Lys Tyr Pro Val Phe Gly Leu Lys Lys Glu Ile Glu Asn Tyr 
            500                 505                 510         


Arg Trp Asn Tyr Val Gln Asp Lys Pro Tyr Pro Cys Asn Glu Thr Arg 
        515                 520                 525             


Leu Leu Met Leu Ala Arg Leu Glu Lys Cys Asp Ile Ser Ile Glu Phe 
    530                 535                 540                 


Leu Thr Lys Glu Asn Glu Glu Ala Leu Trp His Ile Leu Tyr Ser Val 
545                 550                 555                 560 


Glu Asp Lys Asn Glu Ile Val Lys Ala Leu Gln Lys Phe Ala Asp Lys 
                565                 570                 575     


His Asp Leu Asn Ala Ser Phe Val Asp Val Phe Arg Lys Phe Pro Pro 
            580                 585                 590         


Phe Lys Lys Glu Tyr Gly Ser Tyr Ser Ala Lys Ala Ile Lys Lys Leu 
        595                 600                 605             


Leu Pro Leu Met Arg Met Gly Lys Tyr Trp Ser Glu Asp Ser Ile Asp 
    610                 615                 620                 


Lys Gly Thr Arg Lys Arg Met Asp Lys Ile Ile Thr Gly Glu Tyr Asp 
625                 630                 635                 640 


Glu Lys Ile Glu Asn Arg Val Arg Glu Lys Val Ile His Leu Ala Asp 
                645                 650                 655     


Val Ser Asp Phe Arg Gly Leu Pro Leu Trp Leu Val Cys Tyr Ile Val 
            660                 665                 670         


Tyr Gly Arg His Ser Glu Ala Lys Glu Ile Thr Gln Trp Lys Ser Pro 
        675                 680                 685             


Ala Asp Ile Asp Ser Tyr Leu Lys Ser Phe Lys Gln His Ser Leu Arg 
    690                 695                 700                 


Asn Pro Ile Val Glu Gln Ile Ile Ile Glu Thr Leu Arg Val Val Arg 
705                 710                 715                 720 


Asp Ile Trp Lys Gln Val Gly Asn Ile Asp Glu Ile His Val Glu Leu 
                725                 730                 735     


Gly Arg Glu Met Lys Asn Pro Ala Asp Lys Arg Lys Lys Met Thr Gln 
            740                 745                 750         


Gln Met Ser Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys Tyr Leu Leu 
        755                 760                 765             


Thr Glu Phe Leu Asn Pro Glu Tyr Glu Val Glu Asn Val Arg Pro Tyr 
    770                 775                 780                 


Ser Pro Ser Gln Gln Asp Ile Leu Arg Ile Tyr Glu Glu Gly Val Leu 
785                 790                 795                 800 


Asn Ser Val Ser Asp Leu Pro Glu Glu Ile Ser Asp Ile Leu Lys Lys 
                805                 810                 815     


Phe Thr Glu Thr Asp Leu Lys Lys Arg Pro Ser Arg Ser Glu Ile Leu 
            820                 825                 830         


Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly 
        835                 840                 845             


Glu Val Ile Pro Leu Gly Lys Leu Phe Thr Pro Ala Tyr Glu Ile Glu 
    850                 855                 860                 


His Val Ile Pro Gln Ser Cys Tyr Phe Asp Asp Ser Phe Ser Asn Lys 
865                 870                 875                 880 


Val Ile Cys Glu Ala Glu Val Asn Lys Leu Lys Ser Asn Leu Leu Gly 
                885                 890                 895     


His Glu Phe Ile Val Lys His His Gly Glu Ile Val Glu Leu Pro Phe 
            900                 905                 910         


Gly Lys Lys Val Arg Ile Phe Thr Val Asp Glu Tyr Glu Gln Phe Val 
        915                 920                 925             


Lys Asp Asn Tyr Ser Arg Thr Arg Gly Lys Met Lys Lys Leu Leu Met 
    930                 935                 940                 


Asp Asp Ile Pro Glu Glu Phe Val Ala Arg Gln Leu Asn Asp Ser Arg 
945                 950                 955                 960 


Tyr Ile Ser Lys Val Val Lys Ser Leu Leu Ser Asn Ile Val Arg Glu 
                965                 970                 975     


Lys Gly Glu Glu Glu Ala Ile Ser Lys Asn Val Ile Pro Cys Thr Gly 
            980                 985                 990         


Gly Val Thr Asp Arg Leu Lys Lys  Asp Trp Gly Ile Asn  Glu Val Trp 
        995                 1000                 1005             


Asn Lys  Ile Ile Leu Pro Arg  Phe Gln Arg Leu Asn  Glu Leu Thr 
    1010                 1015                 1020             


Gly Thr  Asn Lys Phe Thr Thr  Lys Asn Val Gly Ile  Gln Glu Ile 
    1025                 1030                 1035             


Pro Thr  Met Pro Leu Glu Leu  Gln Lys Gly Phe Asn  Lys Lys Arg 
    1040                 1045                 1050             


Ile Asp  His Arg His His Ala  Met Asp Ala Ile Ile  Ile Ala Cys 
    1055                 1060                 1065             


Ala Asn  Arg Asn Ile Ile Asn  Tyr Leu Asn Asn Glu  Ser Ala Thr 
    1070                 1075                 1080             


Ala Lys  Ala Glu Leu Ser Arg  Tyr Asp Leu Gln Lys  Leu Leu Cys 
    1085                 1090                 1095             


Asp Lys  Ala Lys Thr Asp Asn  Asn Gly Asn Tyr Lys  Trp Val Ile 
    1100                 1105                 1110             


Arg Lys  Pro Trp Ala Ser Phe  Thr Gln Asp Thr Tyr  Leu Ala Leu 
    1115                 1120                 1125             


Glu Asn  Ile Ile Val Ser Phe  Lys Gln Asn Leu Arg  Val Ile Asn 
    1130                 1135                 1140             


Lys Ala  Thr Asn Arg Phe Leu  His Tyr Asn Glu Glu  Gly Lys Lys 
    1145                 1150                 1155             


Ile Phe  Val Lys Gln Gly Lys  Gly Asp Asn Trp Ala  Ile Arg Lys 
    1160                 1165                 1170             


Ser Met  His Lys Asp Thr Val  Phe Gly Glu Val Asn  Leu Arg Arg 
    1175                 1180                 1185             


Ile Lys  Thr Val Ala Leu Asn  Glu Ala Met Lys Asn  Pro Gln Ser 
    1190                 1195                 1200             


Ile Val  Val Lys Asp Phe Lys  Arg Lys Leu Leu Glu  Leu Trp Asn 
    1205                 1210                 1215             


Leu Gly  Phe Asp Ala Lys Arg  Ile Lys Lys Tyr Phe  Glu Asp Asn 
    1220                 1225                 1230             


Arg Glu  Thr Trp Ser Asp Ile  Asn Leu Ser Lys Ile  Glu Val Tyr 
    1235                 1240                 1245             


Tyr Phe  Ser Lys Asp Thr Lys  Asp Arg Phe Phe Ala  Thr Arg Lys 
    1250                 1255                 1260             


Pro Leu  Asp Thr Ser Phe Asp  Arg Lys Lys Ile Glu  Asn Asn Ile 
    1265                 1270                 1275             


Thr Asp  Thr Gly Ile Gln Lys  Ile Leu Leu Arg His  Leu Glu Leu 
    1280                 1285                 1290             


Lys Asp  Asn Asn Pro Asp Ile  Ala Phe Ser Pro Asp  Gly Ile Asp 
    1295                 1300                 1305             


Glu Met  Asn Arg Asn Ile Ile  Gln Leu Asn Asn Gly  Lys Tyr His 
    1310                 1315                 1320             


Gln Pro  Ile Ile Lys Ile Arg  Trp Tyr Glu Gln Ala  Asp Lys Phe 
    1325                 1330                 1335             


Ala Val  Gly Gln Thr Gly Asn  Lys Phe Ser Lys Phe  Val Glu Ala 
    1340                 1345                 1350             


Ala Lys  Gly Thr Asn Leu Phe  Phe Ala Val Tyr Glu  Ser Asn Ile 
    1355                 1360                 1365             


Leu Asp  Lys Lys Thr Asn Thr  Ile Ile Lys Lys Arg  Asn Tyr Ala 
    1370                 1375                 1380             


Thr Ile  Pro Leu Asn Val Ala  Ile Glu Arg Gln Lys  Gln Gly Leu 
    1385                 1390                 1395             


Pro Val  Ala Pro Glu Asp Glu  Asn Gly Asn Asp Pro  Ile Phe Val 
    1400                 1405                 1410             


Leu Ser  Pro Asn Asp Leu Val  Tyr Leu Pro Thr Asp  Asp Glu Leu 
    1415                 1420                 1425             


Ala Asn  Gly Ile Ile Ala Gln  Pro Leu Asp Arg Gly  Arg Ile Tyr 
    1430                 1435                 1440             


Lys Met  Val Ser Cys Thr Gly  Asn Glu Gly His Phe  Ile Pro Ala 
    1445                 1450                 1455             


Arg Ile  Ala Asn Pro Ile Leu  Gln Thr Ile Glu Leu  Gly Ser Asn 
    1460                 1465                 1470             


Asn Lys  Ala Gln Lys Ala Trp  Thr Asp Glu Met Ile  Lys Glu Ile 
    1475                 1480                 1485             


Cys Met  Pro Ile Lys Val Asp  Arg Leu Gly Asn Val  Leu Glu Ser 
    1490                 1495                 1500             


Ser Ser  Ser Tyr Lys Lys 
    1505                 


<210>  1311
<211>  1509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1311

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ser Leu Ile Glu His Asp Phe Lys Asn Lys Gln Gly Gln Ile Glu Gly 
            20                  25                  30          


Leu Gly Val Arg Ile Ile Pro Met Ser Gln Glu Ile Leu Gly Lys Phe 
        35                  40                  45              


Asp Ala Gly Gln Ser Ile Ser Gln Thr Ala Asp Arg Thr Lys Tyr Arg 
    50                  55                  60                  


Gly Val Arg Arg Leu Tyr Gln Arg Asp Asn Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu Lys Ile Leu Asp Phe Leu Pro Lys His Tyr Ser Glu 
                85                  90                  95      


Ser Ile Asp Phe Gln Asp Lys Val Gly Gln Phe Lys Pro Lys Gln Glu 
            100                 105                 110         


Val Lys Leu Asn Tyr Arg Lys Asn Glu Lys Asn Lys His Glu Phe Val 
        115                 120                 125             


Phe Met Asn Ser Phe Ile Glu Met Val Ser Glu Phe Lys Asn Ala Gln 
    130                 135                 140                 


Pro Glu Leu Phe Tyr Asn Lys Gly Asn Gly Glu Glu Thr Lys Ile Pro 
145                 150                 155                 160 


Tyr Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Gln 
                165                 170                 175     


Ile Thr Lys Glu Glu Leu Ala Trp Leu Ile Leu Asn Phe Asn Gln Lys 
            180                 185                 190         


Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Asp Ile Asp Glu Asp Lys Asn 
        195                 200                 205             


Lys Lys Tyr Met Gln Leu Lys Val Asn Asn Leu Ile Asp Ser Gly Ala 
    210                 215                 220                 


Lys Val Lys Gly Lys Val Leu Tyr Asn Val Ile Phe Asp Asn Gly Trp 
225                 230                 235                 240 


Lys Tyr Glu Lys Gln Ile Val Asn Lys Asp Glu Trp Glu Gly Arg Thr 
                245                 250                 255     


Lys Glu Phe Ile Ile Thr Thr Lys Thr Leu Lys Asn Gly Asn Ile Lys 
            260                 265                 270         


Arg Thr Tyr Lys Ala Val Asp Ser Glu Ile Asp Trp Ala Ala Ile Lys 
        275                 280                 285             


Ala Lys Thr Glu Gln Asp Ile Asn Lys Ala Asn Lys Thr Val Gly Glu 
    290                 295                 300                 


Tyr Ile Tyr Glu Ser Leu Leu Asp Asn Pro Ser Gln Lys Ile Arg Gly 
305                 310                 315                 320 


Lys Leu Val Lys Thr Ile Glu Arg Lys Phe Tyr Lys Glu Glu Phe Glu 
                325                 330                 335     


Lys Leu Leu Ser Lys Gln Ile Glu Leu Gln Pro Glu Leu Phe Asn Glu 
            340                 345                 350         


Ser Leu Tyr Lys Ala Cys Ile Lys Glu Leu Tyr Pro Arg Asn Glu Asn 
        355                 360                 365             


His Gln Ser Asn Asn Lys Lys Gln Gly Phe Glu Tyr Leu Phe Thr Glu 
    370                 375                 380                 


Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Gln Lys Ser Asn Ile 
385                 390                 395                 400 


Ser Gly Cys Gln Phe Glu His Lys Ile Tyr Lys Gln Lys Asn Lys Lys 
                405                 410                 415     


Thr Gly Lys Leu Glu Leu Ile Lys Glu Pro Ile Lys Thr Ile Ser Arg 
            420                 425                 430         


Ser His Pro Leu Phe Gln Glu Phe Arg Ile Trp Gln Trp Leu Gln Asn 
        435                 440                 445             


Leu Lys Ile Tyr Asn Lys Glu Lys Ile Glu Asn Gly Lys Leu Glu Asp 
    450                 455                 460                 


Val Thr Thr Gln Leu Leu Pro Asn Asn Glu Ala Tyr Val Thr Leu Phe 
465                 470                 475                 480 


Asp Phe Leu Asn Thr Lys Lys Glu Leu Glu Gln Lys Gln Phe Ile Glu 
                485                 490                 495     


Tyr Phe Val Lys Lys Lys Leu Ile Asp Lys Lys Glu Lys Glu His Phe 
            500                 505                 510         


Arg Trp Asn Phe Val Glu Asp Lys Lys Tyr Pro Phe Ser Glu Thr Arg 
        515                 520                 525             


Ala Gln Phe Leu Ser Arg Leu Ala Lys Val Lys Gly Ile Lys Asn Thr 
    530                 535                 540                 


Glu Asp Phe Leu Asn Lys Asn Thr Gln Val Gly Ser Lys Glu Asn Ser 
545                 550                 555                 560 


Pro Phe Ile Lys Arg Ile Glu Gln Leu Trp His Ile Ile Tyr Ser Val 
                565                 570                 575     


Ser Asp Leu Lys Glu Tyr Glu Lys Ala Leu Glu Lys Phe Ala Glu Lys 
            580                 585                 590         


His Asn Leu Glu Lys Asp Ser Phe Leu Lys Asn Phe Lys Lys Phe Pro 
        595                 600                 605             


Pro Phe Val Ser Asp Tyr Ala Ser Tyr Ser Lys Lys Ala Ile Ser Lys 
    610                 615                 620                 


Leu Leu Pro Ile Met Arg Met Gly Lys Tyr Trp Ser Glu Ser Ala Val 
625                 630                 635                 640 


Pro Thr Gln Val Lys Glu Arg Ser Leu Ser Ile Met Glu Arg Val Lys 
                645                 650                 655     


Val Leu Pro Leu Lys Glu Gly Tyr Ser Asp Lys Asp Leu Ala Asp Leu 
            660                 665                 670         


Leu Ser Arg Val Ser Asp Asp Asp Ile Pro Lys Gln Leu Ile Lys Ser 
        675                 680                 685             


Phe Ile Ser Phe Lys Asp Lys Asn Pro Leu Lys Gly Leu Asn Thr Tyr 
    690                 695                 700                 


Gln Ala Asn Tyr Leu Val Tyr Gly Arg His Ser Glu Thr Gly Asp Ile 
705                 710                 715                 720 


Gln His Trp Lys Thr Pro Glu Asp Ile Asp Arg Tyr Leu Asn Asn Phe 
                725                 730                 735     


Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val Met Glu 
            740                 745                 750         


Thr Leu Arg Val Val Arg Asp Ile Trp Glu His Tyr Gly Asn Asn Glu 
        755                 760                 765             


Lys Asp Phe Phe Lys Glu Ile His Val Glu Leu Gly Arg Glu Met Lys 
    770                 775                 780                 


Ser Pro Ala Gly Lys Arg Glu Lys Leu Ser Gln Arg Asn Thr Glu Asn 
785                 790                 795                 800 


Glu Asn Thr Asn His Arg Ile Arg Glu Val Leu Lys Glu Leu Met Asn 
                805                 810                 815     


Asp Ala Ser Val Glu Gly Gly Val Arg Asp Tyr Ser Pro Ser Gln Gln 
            820                 825                 830         


Glu Ile Leu Lys Leu Tyr Glu Glu Gly Ile Tyr Gln Asn Pro Asn Thr 
        835                 840                 845             


Asn Tyr Leu Lys Val Asp Glu Asp Glu Ile Leu Lys Ile Arg Lys Lys 
    850                 855                 860                 


Asn Asn Pro Thr Gln Lys Glu Ile Gln Arg Tyr Lys Leu Trp Leu Glu 
865                 870                 875                 880 


Gln Gly Tyr Ile Ser Pro Tyr Thr Gly Lys Ile Ile Pro Leu Thr Lys 
                885                 890                 895     


Leu Phe Thr His Glu Tyr Gln Ile Glu His Ile Ile Pro Gln Ser Arg 
            900                 905                 910         


Tyr Tyr Asp Asn Ser Leu Gly Asn Lys Ile Ile Cys Glu Ser Glu Val 
        915                 920                 925             


Asn Glu Asp Lys Asp Asn Lys Thr Ala Tyr Glu Tyr Leu Lys Val Glu 
    930                 935                 940                 


Lys Gly Ser Ile Val Phe Gly His Lys Leu Leu Asn Leu Asp Glu Tyr 
945                 950                 955                 960 


Glu Ala His Val Asn Lys Tyr Phe Lys Lys Asn Lys Thr Lys Leu Lys 
                965                 970                 975     


Asn Leu Leu Ser Glu Asp Ile Pro Glu Gly Phe Ile Asn Arg Gln Leu 
            980                 985                 990         


Asn Asp Ser Arg Tyr Ile Ser Lys  Leu Val Lys Gly Leu  Leu Ser Asn 
        995                 1000                 1005             


Ile Val  Arg Glu Asn Gly Glu  Gln Glu Ala Thr Ser  Lys Asn Leu 
    1010                 1015                 1020             


Ile Pro  Val Thr Gly Val Val  Thr Ser Lys Leu Lys  Gln Asp Trp 
    1025                 1030                 1035             


Gly Leu  Asn Asp Lys Trp Asn  Glu Ile Ile Ala Pro  Arg Phe Lys 
    1040                 1045                 1050             


Arg Leu  Asn Lys Leu Thr Asn  Ser Asn Asp Phe Gly  Phe Trp Asp 
    1055                 1060                 1065             


Asn Asp  Ile Asn Ala Phe Arg  Ile Gln Val Pro Asp  Ser Leu Ile 
    1070                 1075                 1080             


Lys Gly  Phe Ser Lys Lys Arg  Ile Asp His Arg His  His Ala Leu 
    1085                 1090                 1095             


Asp Ala  Leu Val Val Ala Cys  Thr Ser Arg Asn His  Thr His Tyr 
    1100                 1105                 1110             


Leu Ser  Ala Leu Asn Ala Glu  Asn Lys Asn Tyr Ser  Leu Arg Asp 
    1115                 1120                 1125             


Lys Leu  Val Ile Lys Asn Glu  Asn Gly Asp Tyr Thr  Lys Thr Phe 
    1130                 1135                 1140             


Gln Ile  Pro Trp Gln Gly Phe  Thr Ile Glu Ala Lys  Asn Asn Leu 
    1145                 1150                 1155             


Glu Lys  Thr Val Val Ser Phe  Lys Lys Asn Leu Arg  Val Ile Asn 
    1160                 1165                 1170             


Lys Thr  Asn Asn Lys Phe Trp  Ser Tyr Lys Asp Glu  Asn Gly Asn 
    1175                 1180                 1185             


Leu Asn  Leu Gly Lys Asp Gly  Lys Pro Lys Lys Lys  Leu Arg Lys 
    1190                 1195                 1200             


Gln Thr  Lys Gly Tyr Asn Trp  Ala Ile Arg Lys Pro  Leu His Lys 
    1205                 1210                 1215             


Glu Thr  Val Ser Gly Ile Tyr  Asn Ile Asn Ala Pro  Lys Asn Lys 
    1220                 1225                 1230             


Ile Ala  Thr Ser Val Arg Thr  Leu Leu Thr Glu Ile  Lys Asn Glu 
    1235                 1240                 1245             


Lys His  Leu Ala Lys Ile Thr  Asp Leu Arg Ile Arg  Glu Thr Ile 
    1250                 1255                 1260             


Leu Pro  Asn His Leu Lys His  Tyr Leu Asn Asn Lys  Gly Glu Ala 
    1265                 1270                 1275             


Asn Phe  Ser Glu Ala Phe Ser  Gln Gly Gly Ile Glu  Asp Leu Asn 
    1280                 1285                 1290             


Lys Lys  Ile Thr Thr Leu Asn  Glu Gly Lys Lys His  Gln Pro Ile 
    1295                 1300                 1305             


Tyr Arg  Val Lys Ile Phe Glu  Val Gly Ser Lys Phe  Ser Ile Ser 
    1310                 1315                 1320             


Glu Asp  Glu Asn Ser Ala Lys  Ser Lys Lys Tyr Val  Glu Ala Ala 
    1325                 1330                 1335             


Lys Gly  Thr Asn Leu Phe Phe  Ala Ile Tyr Leu Asp  Glu Glu Asn 
    1340                 1345                 1350             


Lys Lys  Arg Asn Tyr Glu Thr  Ile Pro Leu Asn Glu  Val Ile Thr 
    1355                 1360                 1365             


His Gln  Lys Gln Val Ala Gly  Phe Pro Lys Ser Glu  Arg Leu Ser 
    1370                 1375                 1380             


Val Gln  Pro Asp Ser Gln Lys  Gly Thr Phe Leu Phe  Thr Leu Ser 
    1385                 1390                 1395             


Pro Asn  Asp Leu Val Tyr Val  Pro Asn Asn Glu Glu  Leu Glu Asn 
    1400                 1405                 1410             


Arg Asp  Leu Phe Asn Leu Gly  Asn Leu Asn Val Glu  Gln Ile Ser 
    1415                 1420                 1425             


Arg Ile  Tyr Lys Phe Thr Asp  Ser Ser Asp Lys Thr  Cys Asn Phe 
    1430                 1435                 1440             


Ile Pro  Phe Gln Val Ser Lys  Leu Ile Phe Asn Leu  Lys Lys Lys 
    1445                 1450                 1455             


Glu Gln  Lys Lys Leu Asp Val  Asp Phe Ile Ile Gln  Asn Glu Phe 
    1460                 1465                 1470             


Gly Leu  Gly Ser Pro Gln Ser  Lys Asn Gln Lys Ser  Ile Asp Asp 
    1475                 1480                 1485             


Val Met  Ile Lys Glu Lys Cys  Ile Lys Leu Lys Ile  Asp Arg Leu 
    1490                 1495                 1500             


Gly Asn  Ile Ser Lys Ala 
    1505                 


<210>  1312
<211>  1509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1312

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Ser Val Ile Lys Glu Gln Ala Glu Arg Ile Trp Ile 
            20                  25                  30          


Glu Met Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Val Leu Gly 
        35                  40                  45              


Asp Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ser Glu Arg Thr Arg 
    50                  55                  60                  


Leu Arg Gly Val Arg Arg Leu Arg Glu Arg Gln Leu Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Lys Val Leu Asn Ile Leu Gly Phe Leu Pro Asn His Tyr 
                85                  90                  95      


Leu Glu Tyr Ile Asp Phe Glu Lys His Pro Gly Lys Phe Leu Ser Asp 
            100                 105                 110         


Ala Glu Pro Lys Leu Pro Trp Val Lys Asp Glu Gln Gly Lys Tyr Thr 
        115                 120                 125             


Phe Leu Phe Gln Ala Ala Phe Asp Glu Met Leu Ala Asp Phe Ala Ile 
    130                 135                 140                 


His Gln Pro Ser Leu Val Ser Gly Asp Lys Lys Ile Pro Tyr Asp Trp 
145                 150                 155                 160 


Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Glu Met Ile Ser Lys 
                165                 170                 175     


Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr 
            180                 185                 190         


Tyr Gln Leu Arg Gly Glu Glu Glu Glu Asp Asn Ala Gly Lys Ser Val 
        195                 200                 205             


Glu Phe Tyr Ala Leu Lys Val Leu Thr Val Glu Ala Thr Asp Glu Lys 
    210                 215                 220                 


Lys Gly Arg Asp Val Trp Tyr Asn Val His Leu Glu Asn Gly Trp Ile 
225                 230                 235                 240 


Tyr Arg Arg Ser Ser Ser Val Pro Leu Asp Trp Glu Gly Gln Ile Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Asp Leu Asn Pro Asp Ser Thr Pro Lys Leu 
            260                 265                 270         


Asp Lys Tyr Gly Glu Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp 
        275                 280                 285             


Asp Trp Met Leu Ile Lys Lys Arg Thr Glu Ala Asp Ile Ala Asn Thr 
    290                 295                 300                 


His Lys Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Gln Lys Pro 
305                 310                 315                 320 


Gln Gln Lys Ile Ile Gly Lys Leu Val Lys Thr Val Glu Arg Lys Leu 
                325                 330                 335     


Tyr Arg Asp Glu Leu Ser Leu Ile Leu Gln Lys Gln Cys Ala Phe His 
            340                 345                 350         


Ala Glu Leu Gln Asp Arg Thr Leu Tyr Ala Arg Cys Val Glu Ala Leu 
        355                 360                 365             


Tyr Pro Asn Asn Glu Val Gln Arg Arg Asn Ile Ala Asn Arg Asp Phe 
    370                 375                 380                 


Val Tyr Leu Leu Met Asp Asp Val Leu Phe Tyr Gln Arg Pro Leu Lys 
385                 390                 395                 400 


Thr Lys Lys Ser Leu Ile Ala Asn Cys Pro Tyr Glu Glu Asn Glu Tyr 
                405                 410                 415     


Val Asp Arg Glu Thr Gly Glu Ile Lys Lys Ala Pro Leu Lys Cys Ile 
            420                 425                 430         


Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Asn Phe Leu 
        435                 440                 445             


Ser Asn Ile Arg Ile Tyr Gln Lys Glu Lys Glu Ile Asn Gly Thr Leu 
    450                 455                 460                 


His Leu Asp Val Asp Val Thr Ala Glu Phe Leu Lys Thr Glu Asp Asp 
465                 470                 475                 480 


Tyr Val Ala Leu Phe Asp Trp Leu Asn Ser Arg Lys Glu Ile Glu Gln 
                485                 490                 495     


Lys Thr Phe Leu Lys Tyr Pro Lys Phe Gly Leu Lys Lys Glu Ile Ser 
            500                 505                 510         


Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys Gln Tyr Pro Cys Asn Glu 
        515                 520                 525             


Thr Arg His Ala Ile Leu Ser Arg Leu Glu Lys Ala Asn Val Cys Ile 
    530                 535                 540                 


Asp Phe Leu Ser Asn Glu Lys Glu Val Ala Leu Trp His Ile Leu Tyr 
545                 550                 555                 560 


Ser Val Ser Asp Lys Gln Glu Ile Glu Lys Ala Leu Arg Thr Phe Ala 
                565                 570                 575     


Ala Lys Asn Gly Leu Asp Glu Ser Phe Val Glu Ala Phe Lys Lys Phe 
            580                 585                 590         


Pro Pro Phe Lys Ser Glu Tyr Gly Ala Tyr Ser Ala Lys Ala Ile Lys 
        595                 600                 605             


Lys Leu Leu Pro Leu Met Arg Cys Gly Lys Tyr Trp Asp Val Ser Ser 
    610                 615                 620                 


Ile Asp Asp Thr Thr Lys Glu Arg Ile Lys Lys Ile Val Ala Gly Glu 
625                 630                 635                 640 


Tyr Asp Glu Arg Ile Ala Val Lys Val Arg Asp Lys Val Met Asn Leu 
                645                 650                 655     


Ser Asp Ile Ser Cys Phe Lys Arg Leu Pro Leu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Ile Val Tyr Asn Arg His Ser Glu Asp Lys Glu Ala Val Lys Trp Glu 
        675                 680                 685             


Ser Pro Ala Asp Ile Asp Ala Tyr Leu Ala Ser Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val 
705                 710                 715                 720 


Val Arg Asp Ile Trp Lys Gln Val Gly Arg Ile Asp Glu Ile His Ile 
                725                 730                 735     


Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Asp Lys Arg Lys Arg Met 
            740                 745                 750         


Thr Ala Gln Ile Leu Glu Asn Glu Asn Ala Asn Leu Arg Ile Lys Ala 
        755                 760                 765             


Leu Leu Ala Glu Phe Met Asn Pro Glu Tyr Glu Ile Glu Asn Val Arg 
    770                 775                 780                 


Pro Tyr Ser Pro Ser Gln Gln Glu Ile Leu Arg Ile Tyr Glu Asp Met 
785                 790                 795                 800 


Val Leu His Ala Glu Asp Gln Leu Pro Glu Glu Ile Asp Ala Ile Leu 
                805                 810                 815     


Lys Lys Phe Asn Ser Ser Lys Val Pro Thr Lys Ser Glu Phe Leu Arg 
            820                 825                 830         


Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Glu 
        835                 840                 845             


Leu Ile Pro Leu Gly Lys Leu Phe Thr Ala Ala Tyr Glu Ile Glu His 
    850                 855                 860                 


Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val 
865                 870                 875                 880 


Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp Asn Gln Leu Gly Tyr 
                885                 890                 895     


Glu Phe Ile Lys Asn His His Gly Gln Lys Val Glu Val Gly Phe Gly 
            900                 905                 910         


Lys Ser Val Glu Ile Phe Ser Val Asp Ser Tyr Glu Arg Phe Val Lys 
        915                 920                 925             


Glu Gln Tyr Ala Lys Ser Ala Val Lys Met Lys Lys Met Leu Met Asp 
    930                 935                 940                 


Asp Ile Pro Glu Gln Phe Ile Glu Arg Gln Leu Asn Asp Ser Arg Tyr 
945                 950                 955                 960 


Ile Ser Lys Val Ile Lys Gly Leu Leu Ser Asn Ile Val Arg Glu Lys 
                965                 970                 975     


Asn Asp Ser Gly Glu Tyr Glu Pro Glu Ala Val Ser Lys Asn Ile Val 
            980                 985                 990         


Val Cys Thr Gly Ser Val Thr Asp  Arg Leu Lys Arg Asp  Trp Gly Met 
        995                 1000                 1005             


Asn Asp  Val Trp Asn Ser Ile  Val Tyr Pro Arg Phe  Glu Arg Leu 
    1010                 1015                 1020             


Asn Ser  Leu Thr Asp Thr Gln  Ser Phe Gly His Trp  Glu Asn Lys 
    1025                 1030                 1035             


Ser Gly  Lys Lys Val Phe Gln  Thr Glu Leu Pro Leu  Glu Tyr Gln 
    1040                 1045                 1050             


Lys Gly  Phe Ser Lys Lys Arg  Ile Asp His Arg His  His Ala Met 
    1055                 1060                 1065             


Asp Ala  Ile Val Ile Ala Cys  Ala Thr Arg Asn His  Val Asn Tyr 
    1070                 1075                 1080             


Leu Ser  Asn Glu Ser Ala Ser  Arg Asn Ala Lys Ile  Ser Arg Tyr 
    1085                 1090                 1095             


Asp Leu  Gln Arg Leu Leu Cys  Asp Lys Ser Arg Thr  Asp Asp Asn 
    1100                 1105                 1110             


Gly Asn  Tyr Lys Trp Val Ile  Lys Lys Pro Trp Asp  Thr Phe Thr 
    1115                 1120                 1125             


Gln Asp  Ala Arg Glu Val Leu  Ser Lys Ile Val Val  Ser Leu Lys 
    1130                 1135                 1140             


Gln Asn  Leu Arg Ile Ile Asn  Lys Thr Thr Asn Val  Tyr Arg His 
    1145                 1150                 1155             


Leu Asp  Ala Thr Gly Asn Arg  Val Tyr Glu Lys Gln  Lys Thr Gly 
    1160                 1165                 1170             


Asp Ser  Trp Ala Ile Arg Lys  Pro Met His Lys Asp  Thr Val Phe 
    1175                 1180                 1185             


Gly Ala  Val Asn Leu Arg Lys  Val Lys Val Val Arg  Leu Ser Val 
    1190                 1195                 1200             


Ala Leu  Asp Ala Pro Thr Met  Ile Val Asp Lys Arg  Val Lys Asn 
    1205                 1210                 1215             


Lys Val  Phe Glu Leu Leu Ser  Tyr Lys Tyr Asp Lys  Lys Arg Ile 
    1220                 1225                 1230             


Glu Lys  Tyr Phe Lys Glu Asn  Ala Val Leu Trp Lys  Glu Leu Asp 
    1235                 1240                 1245             


Leu Ala  Arg Val Ala Val Tyr  Tyr Phe Thr Glu Ala  Thr Thr Glu 
    1250                 1255                 1260             


Pro Leu  Val Ala Val Arg Lys  Pro Leu Asp Ser Thr  Phe Asn Glu 
    1265                 1270                 1275             


Lys Lys  Ile Lys Glu Ser Val  Thr Asp Thr Gly Ile  Gln Lys Ile 
    1280                 1285                 1290             


Leu Leu  Asn His Leu Cys Thr  Lys Glu Gly Lys Pro  Asp Leu Ala 
    1295                 1300                 1305             


Phe Ser  Ala Glu Gly Ile Glu  Glu Met Asn Arg Asn  Leu Leu Gln 
    1310                 1315                 1320             


Leu Asn  Asn Gly Lys Arg His  Gln Pro Ile Tyr Lys  Val Arg Val 
    1325                 1330                 1335             


Tyr Glu  Pro Arg Gly Asn Lys  Phe Ser Val Gly Val  Leu Gly Asn 
    1340                 1345                 1350             


Lys Gly  Thr Lys Trp Val Glu  Ala Ala Lys Gly Thr  Asn Leu Phe 
    1355                 1360                 1365             


Phe Ala  Ile Tyr Ile Thr Glu  Asp Gly Lys Arg Thr  Tyr Glu Thr 
    1370                 1375                 1380             


Ile Pro  Leu Asn Met Val Ile  Glu Arg Glu Lys Gln  Gly Leu Ala 
    1385                 1390                 1395             


Pro Val  Pro Asp Lys Asn Glu  Lys Gly Asp Lys Leu  Leu Phe Trp 
    1400                 1405                 1410             


Leu Ser  Pro Asn Asp Leu Val  Tyr Leu Pro Thr Glu  Glu Glu Leu 
    1415                 1420                 1425             


Glu Phe  Gly Arg Ile Asn Glu  Pro Ile Asp Arg Gly  Arg Ile Tyr 
    1430                 1435                 1440             


Lys Met  Val Ser Ser Ser Gly  Asn Gln Cys Phe Phe  Ile Pro Gln 
    1445                 1450                 1455             


Ser Ile  Ser Asn Ala Ile Ile  Pro Thr Lys Glu Leu  Gly Ala Asn 
    1460                 1465                 1470             


Asn Lys  Ala Glu Arg Ser Trp  Asp Gly Ala Met Ile  Lys Asn Ile 
    1475                 1480                 1485             


Cys Ile  Pro Val Lys Val Asp  Arg Leu Gly Arg Ile  Ile Glu Val 
    1490                 1495                 1500             


Lys Tyr  Arg Ile Asn Glu 
    1505                 


<210>  1313
<211>  1509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1313

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Ser Val Ile Lys Glu Gln Ala Glu Arg Ile Trp Ile 
            20                  25                  30          


Glu Met Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Val Leu Gly 
        35                  40                  45              


Asp Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ser Glu Arg Thr Arg 
    50                  55                  60                  


Leu Arg Gly Ala Arg Arg Leu Arg Glu Arg Gln Leu Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Lys Val Leu Asn Ile Leu Gly Phe Leu Pro Asn His Tyr 
                85                  90                  95      


Leu Glu Tyr Ile Asp Phe Glu Lys His Pro Gly Lys Phe Leu Ala Asp 
            100                 105                 110         


Ala Glu Pro Lys Leu Ala Trp Val Lys Asp Glu Gln Gly Lys Tyr Thr 
        115                 120                 125             


Phe Leu Phe Gln Ala Ala Phe Asp Glu Met Leu Ala Asp Phe Ala Ile 
    130                 135                 140                 


His Gln Pro Ser Leu Val Ser Gly Asp Lys Lys Ile Pro Tyr Asp Trp 
145                 150                 155                 160 


Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Glu Met Ile Ser Lys 
                165                 170                 175     


Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr 
            180                 185                 190         


Tyr Gln Leu Arg Gly Glu Glu Glu Glu Asp Asn Ala Gly Lys Ser Val 
        195                 200                 205             


Glu Phe Tyr Ala Leu Lys Val Leu Thr Val Glu Ala Thr Asp Glu Lys 
    210                 215                 220                 


Lys Gly Arg Asp Val Trp Tyr Asn Val His Leu Glu Asn Gly Trp Ile 
225                 230                 235                 240 


Tyr Arg Arg Ser Ser Ser Val Pro Leu Asp Trp Glu Gly Gln Val Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Asp Leu Asn Pro Asp Gly Thr Pro Lys Leu 
            260                 265                 270         


Asp Lys Tyr Gly Glu Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp 
        275                 280                 285             


Asp Trp Met Leu Ile Lys Lys Arg Thr Glu Ala Asp Ile Ala Asn Thr 
    290                 295                 300                 


His Lys Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Gln Lys Pro 
305                 310                 315                 320 


Gln Gln Lys Ile Ile Gly Lys Leu Val Lys Thr Val Glu Arg Lys Leu 
                325                 330                 335     


Tyr Arg Asp Glu Leu Ser Leu Ile Leu Gln Lys Gln Cys Ala Phe His 
            340                 345                 350         


Ala Glu Leu Gln Asp Arg Ala Leu Tyr Ala Arg Cys Val Glu Ala Leu 
        355                 360                 365             


Tyr Pro Ser Asn Glu Val Arg Arg Lys Asn Ile Ala Asn Arg Asp Phe 
    370                 375                 380                 


Val Tyr Leu Leu Met Asp Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys 
385                 390                 395                 400 


Thr Lys Lys Ser Leu Ile Ala Asn Cys Pro Tyr Glu Glu Asn Glu Tyr 
                405                 410                 415     


Val Asp Arg Glu Thr Gly Glu Ile Lys Lys Thr Pro Leu Lys Cys Ile 
            420                 425                 430         


Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Asn Phe Leu 
        435                 440                 445             


Ser Asn Ile Arg Ile Tyr Gln Lys Glu Lys Glu Ile Asn Gly Thr Leu 
    450                 455                 460                 


His Leu Asp Val Asp Val Thr Ala Glu Phe Leu Lys Thr Glu Asp Asp 
465                 470                 475                 480 


Tyr Val Ala Leu Phe Asp Trp Leu Asn Ser Arg Lys Glu Ile Glu Gln 
                485                 490                 495     


Lys Thr Phe Leu Lys Tyr Pro Lys Phe Gly Leu Lys Lys Glu Ile Ser 
            500                 505                 510         


Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys Gln Tyr Pro Cys Asn Glu 
        515                 520                 525             


Thr Arg His Ala Ile Leu Ser Ser Leu Glu Lys Ala Asn Val Cys Ile 
    530                 535                 540                 


Asp Phe Leu Ser Asn Glu Lys Glu Val Ala Leu Trp His Ile Leu Tyr 
545                 550                 555                 560 


Ser Val Ser Asp Lys Gln Glu Ile Glu Lys Ala Leu Arg Thr Phe Ala 
                565                 570                 575     


Ala Lys Asn Gly Leu Asp Glu Ser Phe Val Glu Ala Phe Lys Lys Phe 
            580                 585                 590         


Pro Pro Phe Lys Ser Glu Tyr Gly Ala Tyr Ser Ala Lys Ala Ile Lys 
        595                 600                 605             


Lys Met Leu Pro Leu Met Arg Cys Gly Lys Tyr Trp Asp Val Ser Ser 
    610                 615                 620                 


Ile Asp Asp Thr Thr Lys Glu Arg Ile Lys Lys Ile Val Thr Gly Glu 
625                 630                 635                 640 


Tyr Asp Glu Arg Ile Ser Val Lys Val Arg Asp Lys Val Met Asn Leu 
                645                 650                 655     


Ser Asp Ile Ser Cys Phe Arg Arg Leu Pro Leu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Ile Val Tyr Asn Arg His Ser Glu Asp Lys Glu Ala Val Lys Trp Glu 
        675                 680                 685             


Ser Pro Ala Asp Ile Asp Ala Tyr Leu Ala Ser Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val 
705                 710                 715                 720 


Val Arg Asp Ile Trp Lys Gln Val Gly Arg Ile Asp Glu Ile His Ile 
                725                 730                 735     


Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Asp Lys Arg Lys Arg Met 
            740                 745                 750         


Thr Ala Gln Ile Leu Glu Asn Glu Asn Ala Asn Leu Arg Ile Lys Ala 
        755                 760                 765             


Leu Leu Ala Glu Phe Met Asn Pro Glu Tyr Glu Ile Glu Asn Val Arg 
    770                 775                 780                 


Pro Tyr Ser Pro Ser Gln Gln Glu Ile Leu Arg Ile Tyr Glu Asp Thr 
785                 790                 795                 800 


Val Leu His Ala Glu Asp Gln Leu Pro Glu Glu Ile Asp Ala Ile Leu 
                805                 810                 815     


Lys Lys Phe Asn Ser Ser Lys Val Pro Thr Lys Ser Glu Phe Leu Arg 
            820                 825                 830         


Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Glu 
        835                 840                 845             


Leu Ile Pro Leu Gly Lys Leu Phe Thr Ala Ala Tyr Glu Ile Glu His 
    850                 855                 860                 


Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val 
865                 870                 875                 880 


Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp Asn Gln Leu Gly Tyr 
                885                 890                 895     


Glu Phe Ile Lys Asn His His Gly Gln Lys Val Glu Val Gly Phe Gly 
            900                 905                 910         


Lys Ser Val Glu Ile Phe Ser Val Asp Ser Tyr Glu Arg Phe Val Lys 
        915                 920                 925             


Glu Gln Tyr Ala Lys Ser Ala Val Lys Met Lys Lys Leu Leu Met Asp 
    930                 935                 940                 


Asp Ile Pro Glu Gln Phe Ile Glu Arg Gln Leu Asn Asp Ser Arg Tyr 
945                 950                 955                 960 


Ile Ser Lys Val Ile Lys Gly Leu Leu Ser Asn Ile Val Arg Glu Lys 
                965                 970                 975     


Asn Asp Ser Gly Glu Tyr Glu Pro Glu Ala Val Ser Lys Asn Ile Val 
            980                 985                 990         


Val Cys Thr Gly Ser Val Thr Asp  Arg Leu Lys Arg Asp  Trp Gly Met 
        995                 1000                 1005             


Asn Asp  Val Trp Asn Ser Ile  Val Tyr Pro Arg Phe  Glu Arg Leu 
    1010                 1015                 1020             


Asn Ser  Leu Thr Asp Thr Gln  Ser Phe Gly His Trp  Glu Asn Lys 
    1025                 1030                 1035             


Ser Gly  Lys Lys Val Phe Gln  Thr Glu Leu Pro Leu  Glu Tyr Gln 
    1040                 1045                 1050             


Lys Gly  Phe Ser Lys Lys Arg  Ile Asp His Arg His  His Ala Met 
    1055                 1060                 1065             


Asp Ala  Ile Val Ile Ala Cys  Ala Thr Arg Asn His  Val Asn Tyr 
    1070                 1075                 1080             


Leu Ser  Asn Glu Ser Ala Ser  Arg Asn Ala Lys Ile  Ser Arg Tyr 
    1085                 1090                 1095             


Asp Leu  Gln Arg Leu Leu Cys  Asp Lys Asn Arg Thr  Asp Asp Asn 
    1100                 1105                 1110             


Gly Asn  Tyr Lys Trp Val Ile  Lys Lys Pro Trp Asp  Thr Phe Thr 
    1115                 1120                 1125             


Gln Asp  Ala Arg Glu Val Leu  Ser Lys Ile Val Val  Ser Leu Lys 
    1130                 1135                 1140             


Gln Asn  Leu Arg Ile Ile Asn  Lys Thr Thr Asn Val  Tyr Arg His 
    1145                 1150                 1155             


Leu Asp  Ala Thr Gly Asn Arg  Val Tyr Glu Lys Gln  Lys Thr Gly 
    1160                 1165                 1170             


Asp Ser  Trp Ala Ile Arg Lys  Pro Met His Lys Asp  Thr Val Phe 
    1175                 1180                 1185             


Gly Ala  Val Asn Leu Arg Lys  Val Lys Val Val Arg  Leu Ser Val 
    1190                 1195                 1200             


Ala Leu  Asp Thr Pro Ala Met  Ile Val Asp Lys Arg  Val Lys Asn 
    1205                 1210                 1215             


Lys Val  Phe Glu Leu Leu Ser  Tyr Lys Tyr Asp Lys  Lys Arg Ile 
    1220                 1225                 1230             


Glu Lys  Tyr Phe Lys Glu Asn  Ala Phe Leu Trp Lys  Glu Leu Asp 
    1235                 1240                 1245             


Leu Ala  Lys Val Ala Val Tyr  Tyr Phe Thr Glu Ala  Thr Thr Glu 
    1250                 1255                 1260             


Pro Leu  Val Ala Val Arg Lys  Pro Leu Asp Ser Thr  Phe Asn Glu 
    1265                 1270                 1275             


Lys Lys  Ile Lys Glu Ser Val  Thr Asp Thr Gly Ile  Gln Lys Ile 
    1280                 1285                 1290             


Leu Leu  Asn His Leu Cys Thr  Lys Glu Gly Lys Pro  Asp Leu Ala 
    1295                 1300                 1305             


Phe Ser  Ala Glu Gly Ile Glu  Glu Met Asn Arg Asn  Leu Leu Gln 
    1310                 1315                 1320             


Leu Asn  Asn Gly Lys Ser His  Gln Pro Ile Tyr Lys  Val Arg Val 
    1325                 1330                 1335             


Tyr Glu  Pro Arg Gly Asn Lys  Phe Ser Val Gly Val  Leu Gly Asn 
    1340                 1345                 1350             


Lys Gly  Thr Lys Trp Val Glu  Ala Ala Lys Gly Thr  Asn Leu Phe 
    1355                 1360                 1365             


Phe Ala  Ile Tyr Ile Thr Glu  Asp Gly Lys Arg Thr  Tyr Glu Thr 
    1370                 1375                 1380             


Ile Pro  Leu Asn Val Val Ile  Glu Arg Glu Lys Gln  Gly Leu Val 
    1385                 1390                 1395             


Pro Val  Pro Asp Arg Asn Lys  Lys Gly Asp Lys Leu  Leu Phe Trp 
    1400                 1405                 1410             


Leu Ser  Pro Asn Asp Leu Val  Tyr Leu Pro Thr Glu  Glu Glu Arg 
    1415                 1420                 1425             


Glu Phe  Gly Arg Ile Asn Glu  Pro Ile Asp Arg Gly  Arg Ile Tyr 
    1430                 1435                 1440             


Lys Met  Val Ser Cys Thr Gly  Asn Glu Gly His Phe  Ile Pro Ala 
    1445                 1450                 1455             


Asn Val  Ala Asn Pro Ile Val  Pro Val Ile Glu Leu  Gly Ser Asn 
    1460                 1465                 1470             


Asn Lys  Ala Gln Arg Ala Trp  Thr Asn Glu Met Ile  Lys Asp Ile 
    1475                 1480                 1485             


Cys Ile  Pro Ile Lys Val Asp  Arg Leu Gly His Ile  Ile Glu Val 
    1490                 1495                 1500             


Lys Tyr  Arg Ile Asp Glu 
    1505                 


<210>  1314
<211>  1509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1314

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Ser Val Val Asn Asp Glu Gly Lys Glu Gln Leu Val 
            20                  25                  30          


Asn Ile Ser Ser Cys Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Thr 
        35                  40                  45              


Leu Gly Asp Phe Asp Lys Gly Asn Ser Lys Ser Pro Val Ala Glu Arg 
    50                  55                  60                  


Thr Arg Leu Arg Gly Val Arg Arg Leu Leu Glu Arg Ser Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Val Leu Ser Val Met Gly Phe Leu Pro Glu 
                85                  90                  95      


His Tyr Ala Ala Gln Leu Asp Arg Tyr Gly Lys Phe Leu Pro Glu Thr 
            100                 105                 110         


Glu Pro Lys Leu Ala Trp Tyr Lys Asp Glu Thr Gly Lys Tyr Arg Phe 
        115                 120                 125             


Leu Phe Gln Asp Ser Phe Asn Glu Met Leu Glu Asp Phe His Glu His 
    130                 135                 140                 


His Pro Glu Leu Val Ala Glu Gly Lys Lys Ile His Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Lys Ile Thr Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Gln Glu Asp Asn Lys Lys Ser Val Glu 
        195                 200                 205             


Tyr Tyr Ala Leu Lys Val Val Ala Val Glu Asp Ser Gly Asp Arg Lys 
    210                 215                 220                 


Gly Lys Asp Ile Trp Tyr Asn Val Lys Leu Glu Asn Gly Trp Val Tyr 
225                 230                 235                 240 


Arg Arg Ala Ser Gly Val Pro Leu Asp Trp Val Gly Lys Val Lys Glu 
                245                 250                 255     


Phe Ile Val Thr Thr Asp Leu Asp Glu Lys Gly Asn Pro Lys Lys Asp 
            260                 265                 270         


Lys Asp Gly Asn Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp 
        275                 280                 285             


Trp Gly Leu Leu Lys Thr Arg Thr Gln Ala Gln Ile Asp Glu Ser Gly 
    290                 295                 300                 


Gln Thr Val Gly Thr Tyr Ile Tyr Glu Ser Leu Leu Arg Met Pro Asp 
305                 310                 315                 320 


Gln Lys Ile Arg Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Asp Glu Leu Arg Lys Ile Leu Ile Lys Gln Ser Glu Phe His Thr 
            340                 345                 350         


Ala Leu Gln Asp His Asn Leu Leu Leu Ala Cys Ile Glu Glu Leu Tyr 
        355                 360                 365             


Pro Asn Asn Glu Ala His Arg Arg Leu Leu Ser Thr Ser Asp Phe Thr 
    370                 375                 380                 


Tyr Phe Leu Val Glu Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys Ser 
385                 390                 395                 400 


Gln Lys Gly Gln Ile Asp Asp Cys Pro Tyr Glu Ser His Val Tyr Arg 
                405                 410                 415     


Asp Lys Lys Asp Gly Ser Leu Gln His Val Pro Leu Lys Cys Val Ser 
            420                 425                 430         


Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp His Phe Leu Ser 
        435                 440                 445             


Phe Leu Arg Ile Tyr Gln Arg Glu Arg Met Ile Asp Gly Ser Leu Lys 
    450                 455                 460                 


Leu Asp Val Asp Val Thr Asn Glu Phe Leu Gln Asn Glu Asp Asp Tyr 
465                 470                 475                 480 


Val Lys Leu Phe Asp Trp Leu Asn Glu Arg Lys Glu Ile Asp Gln Lys 
                485                 490                 495     


Thr Ile Leu Ala Tyr Lys Pro Phe Gly Leu Lys Lys Asn Glu Gln Ser 
            500                 505                 510         


Lys Tyr Arg Trp Asn Tyr Val Glu Asp Lys Ser Tyr Pro Cys Asn Ser 
        515                 520                 525             


Thr Arg Ala Asp Met Ile Phe Arg Leu Ser Lys Ala Gly Val Pro Glu 
    530                 535                 540                 


Glu Phe Leu Thr Arg Glu Lys Glu Glu Ala Leu Trp His Ile Leu Tyr 
545                 550                 555                 560 


Ser Ile Ser Asp Lys Lys Glu Leu Thr Lys Ala Leu Gly Thr Phe Ala 
                565                 570                 575     


Glu Lys Asn Gly Leu Asn Glu Thr Phe Val Glu Ile Phe Ser Lys Met 
            580                 585                 590         


Pro Pro Phe Glu Ser Asp Tyr Ala Ala Tyr Ser Leu Lys Ala Ile Arg 
        595                 600                 605             


Lys Leu Leu Ala Leu Met Arg Met Gly Lys Tyr Trp Asp Glu Gln Ala 
    610                 615                 620                 


Ile Asp Glu His Thr Arg Ser Arg Ile Glu Lys Ile Leu Thr Gly Glu 
625                 630                 635                 640 


Tyr Asp Glu Thr Ile Arg Thr Arg Val Arg Glu Lys Ala Met His Leu 
                645                 650                 655     


Thr Asp Ile Ser Ser Phe Arg Gly Leu Pro Leu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Val Val Tyr Asp Arg His Ser Glu Ala Lys Glu Leu Val Lys Trp Glu 
        675                 680                 685             


Thr Pro Asp Asp Ile Asp His Phe Leu Arg Lys Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Ile Ile Met Glu Thr Leu Arg Thr 
705                 710                 715                 720 


Val Arg Asp Ile Trp Lys Gln Glu Gly Lys Ile Asp Glu Ile His Val 
                725                 730                 735     


Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Lys Glu Arg Ala Arg Ile 
            740                 745                 750         


Thr Ala Gln Val Leu Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala 
        755                 760                 765             


Leu Leu Ala Glu Phe Met Asn Pro Glu Phe Glu Ile Glu Asn Val Arg 
    770                 775                 780                 


Pro Tyr Ser Pro Ala Gln Gln Glu Ile Leu Arg Ile Tyr Glu Glu Gly 
785                 790                 795                 800 


Val Leu Ser Gly Val Ala Glu Lys Asp Leu Pro Asp Asp Ile Ala Ser 
                805                 810                 815     


Ile Leu Lys Lys Phe Arg Glu Asn Asp Val Lys Lys Arg Pro Thr Thr 
            820                 825                 830         


Ser Glu Val Leu Arg Tyr Lys Leu Trp Leu Glu Gln Arg Tyr Arg Ser 
        835                 840                 845             


Pro Tyr Thr Gly Arg Ile Ile Pro Leu Gly Lys Leu Phe Thr Pro Ala 
    850                 855                 860                 


Tyr Glu Ile Glu His Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser 
865                 870                 875                 880 


Phe Thr Asn Lys Val Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp 
                885                 890                 895     


Asn Cys Leu Gly Tyr Glu Phe Val Lys Lys His Ser Gly Glu Lys Val 
            900                 905                 910         


Glu Leu Gly Asn Gly Glu Thr Ala Thr Ile Phe Ser Val Glu Glu Tyr 
        915                 920                 925             


Glu Arg Phe Val Lys Asp Ser Tyr Phe Asn Asn Ser Lys Lys Ile Lys 
    930                 935                 940                 


Lys Leu Leu Leu Glu Asp Ile Pro Asp Asn Phe Ile Glu Arg Gln Leu 
945                 950                 955                 960 


Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser Leu Leu Ser Asn 
                965                 970                 975     


Ile Val Cys Glu Glu Gly Glu Gln Glu Gly Met Ser Lys Asn Val Ile 
            980                 985                 990         


Val Cys Thr Gly Gly Val Thr Asp  Arg Leu Lys Lys Asp  Trp Gly Val 
        995                 1000                 1005             


Gln Asp  Val Trp Asn Arg Ile  Ile Leu Pro Arg Phe  Leu Arg Leu 
    1010                 1015                 1020             


Asn Lys  Met Thr Asp Arg Thr  Asp Phe Thr Ala Val  Ser Thr Asn 
    1025                 1030                 1035             


Gly His  Leu Leu Pro Ala Val  Pro Leu His Leu Gln  Lys Gly Phe 
    1040                 1045                 1050             


Asn Lys  Lys Arg Ile Asp His  Arg His His Ala Met  Asp Ala Ile 
    1055                 1060                 1065             


Val Ile  Ala Cys Ala Gly Arg  Asn Ile Val Asn Tyr  Leu Asn Asn 
    1070                 1075                 1080             


Ala Ser  Ala Arg Lys Gly Ser  Glu Ile Ser Arg Tyr  Asp Leu Gln 
    1085                 1090                 1095             


His Leu  Leu Cys Asp Lys Val  Lys Thr Asp Ala Asn  Gly Asn Tyr 
    1100                 1105                 1110             


Lys Trp  Ile Leu Arg Lys Pro  Trp Glu Thr Phe Thr  Gln Asp Leu 
    1115                 1120                 1125             


Tyr Ala  Ala Leu Thr Asn Ile  Val Val Ser Phe Lys  Gln Asn Leu 
    1130                 1135                 1140             


Arg Val  Ile Asn Arg Thr Thr  Asn His Tyr Gln His  Tyr Asn Glu 
    1145                 1150                 1155             


Asp Gly  Glu Lys Lys Met Ile  Pro Gln Thr Lys Gly  Asp Arg Leu 
    1160                 1165                 1170             


Ala Ile  Arg Lys Pro Met His  Lys Asp Thr Val Tyr  Gly Glu Val 
    1175                 1180                 1185             


Asn Leu  Arg Lys Glu Lys Thr  Leu Pro Leu Lys Asp  Val Val Lys 
    1190                 1195                 1200             


Asn Pro  Ser Ala Val Val Asp  Lys Ser Leu Lys Asn  Lys Leu Tyr 
    1205                 1210                 1215             


Glu Leu  Leu Lys Ser Gln Tyr  Asp Leu Lys Ala Ile  Thr Lys Tyr 
    1220                 1225                 1230             


Phe Glu  Thr His Gln Asp Val  Trp Ala Asp Val Asn  Leu Lys Lys 
    1235                 1240                 1245             


Ile Lys  Val Tyr Tyr Phe Thr  Lys Glu Thr Asn Glu  Arg Phe Phe 
    1250                 1255                 1260             


Ala Thr  Arg Lys Ser Leu Asp  Ser Ser Phe Ser Gln  Lys Lys Ile 
    1265                 1270                 1275             


Glu Glu  Glu Val Thr Asp Thr  Gly Ile Gln Lys Ile  Leu Leu Arg 
    1280                 1285                 1290             


His Leu  Gln Gln Asn Gly Asn  Asn Pro Asp Ile Ala  Phe Ser Pro 
    1295                 1300                 1305             


Asp Gly  Val Glu Arg Met Asn  Gln Asn Met Thr Ile  Leu Asn Asp 
    1310                 1315                 1320             


Gly Lys  Trp His Gln Pro Ile  Tyr Lys Val Arg Thr  Tyr Glu Lys 
    1325                 1330                 1335             


Ala Asp  Lys Phe Ala Val Gly  Glu Lys Gly Asn Lys  Val Lys Lys 
    1340                 1345                 1350             


Phe Val  Glu Ala Ala Lys Gly  Thr Asn Leu Phe Phe  Ala Val Tyr 
    1355                 1360                 1365             


Glu Ser  Ile Gln Glu Asp Glu  Ala Thr Gly Lys Gln  Val Arg Lys 
    1370                 1375                 1380             


Arg Thr  Phe Ala Thr Ile Pro  Leu Asn Glu Val Ile  Lys Arg Lys 
    1385                 1390                 1395             


Lys Gln  Gly Leu Pro Ala Ala  Pro Glu Asp Ser Lys  Gly Asn Leu 
    1400                 1405                 1410             


Pro Ile  Phe Val Leu Ser Pro  Asn Asp Leu Val Tyr  Leu Pro Thr 
    1415                 1420                 1425             


Glu Glu  Glu Arg Asn Ser Gly  Lys Ile Ile Gln Pro  Leu Asp Arg 
    1430                 1435                 1440             


Glu Arg  Ile Tyr Lys Met Val  Ser Cys Thr Glu Gly  Glu Cys His 
    1445                 1450                 1455             


Cys Ile  Pro Glu Tyr Val Ala  Asn Pro Ile Leu Gln  Thr Ile Glu 
    1460                 1465                 1470             


Leu Gly  Ser Asn Asn Lys Ala  Gln Arg Ala Trp Ser  Gly Glu Met 
    1475                 1480                 1485             


Ile Lys  Glu Ile Cys Val Pro  Val Lys Val Asp Arg  Leu Gly Asn 
    1490                 1495                 1500             


Val Ser  Leu Ile Lys Ile 
    1505                 


<210>  1315
<211>  1509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1315

Met Lys Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Ser Val Ile Lys Glu Gln Ala Glu Arg Ile Trp Ile 
            20                  25                  30          


Glu Thr Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Val Leu Gly 
        35                  40                  45              


Asp Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ser Glu Arg Thr Arg 
    50                  55                  60                  


Leu Arg Gly Ala Arg Arg Leu Arg Glu Arg Gln Leu Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Lys Val Leu Asn Ile Leu Gly Phe Leu Pro Asn His Tyr 
                85                  90                  95      


Leu Glu Tyr Ile Asp Phe Glu Lys His Pro Gly Lys Phe Leu Ala Asp 
            100                 105                 110         


Ala Glu Pro Lys Leu Ala Trp Val Lys Asp Glu Gln Gly Lys Tyr Thr 
        115                 120                 125             


Phe Leu Phe Gln Ala Ala Phe Asp Glu Met Leu Ala Asp Phe Ala Ile 
    130                 135                 140                 


His Gln Pro Ser Leu Val Ser Gly Asp Lys Lys Ile Pro Tyr Asp Trp 
145                 150                 155                 160 


Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Glu Met Ile Ser Lys 
                165                 170                 175     


Glu Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr 
            180                 185                 190         


Tyr Gln Leu Arg Gly Glu Glu Glu Glu Asp Asn Ala Gly Lys Ser Val 
        195                 200                 205             


Glu Phe Tyr Ala Leu Lys Val Leu Thr Val Glu Ala Thr Asp Glu Lys 
    210                 215                 220                 


Lys Gly Arg Asp Val Trp Tyr Asn Val His Leu Glu Asn Gly Trp Ile 
225                 230                 235                 240 


Tyr Arg Arg Ser Ser Ser Val Pro Leu Asp Trp Glu Gly Gln Val Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Asp Leu Asn Pro Asp Gly Thr Pro Lys Leu 
            260                 265                 270         


Asp Lys Tyr Gly Glu Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp 
        275                 280                 285             


Asp Trp Met Leu Ile Lys Lys Arg Thr Glu Ala Asp Ile Ala Asn Thr 
    290                 295                 300                 


His Lys Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Gln Lys Pro 
305                 310                 315                 320 


Gln Gln Lys Ile Ile Gly Lys Leu Val Lys Thr Val Glu Arg Lys Leu 
                325                 330                 335     


Tyr Arg Asp Glu Leu Ser Leu Ile Leu Gln Lys Gln Cys Ala Phe His 
            340                 345                 350         


Ala Glu Leu Gln Asp Arg Ala Leu Tyr Ala Arg Cys Val Glu Ala Leu 
        355                 360                 365             


Tyr Pro Ser Asn Glu Val Arg Arg Lys Asn Ile Ala Asn Arg Asp Phe 
    370                 375                 380                 


Val Tyr Leu Leu Met Asp Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys 
385                 390                 395                 400 


Thr Lys Lys Ser Leu Ile Ala Asn Cys Pro Tyr Glu Glu Asn Glu Tyr 
                405                 410                 415     


Val Asp Arg Glu Thr Gly Glu Ile Lys Lys Ala Pro Leu Lys Cys Ile 
            420                 425                 430         


Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Asn Phe Leu 
        435                 440                 445             


Ser Asn Ile Arg Ile Tyr Gln Lys Glu Lys Glu Ile Asn Gly Thr Leu 
    450                 455                 460                 


His Leu Asp Val Asp Val Thr Ala Glu Phe Leu Lys Thr Glu Asp Asp 
465                 470                 475                 480 


Tyr Val Ala Leu Phe Asp Trp Leu Asn Ser Arg Lys Glu Ile Glu Gln 
                485                 490                 495     


Lys Thr Phe Leu Lys Tyr Pro Lys Phe Gly Leu Lys Lys Glu Ile Ser 
            500                 505                 510         


Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys Gln Tyr Pro Cys Asn Glu 
        515                 520                 525             


Thr Arg His Ala Ile Leu Ser Ser Leu Glu Lys Ala Asn Val Cys Ile 
    530                 535                 540                 


Asp Phe Leu Ser Asn Glu Lys Glu Val Ala Leu Trp His Ile Leu Tyr 
545                 550                 555                 560 


Ser Val Ser Asp Lys Gln Glu Ile Glu Lys Ala Leu Arg Thr Phe Ala 
                565                 570                 575     


Ala Lys Asn Gly Leu Asp Glu Ser Phe Val Glu Ala Phe Lys Lys Phe 
            580                 585                 590         


Pro Pro Phe Lys Ser Glu Tyr Gly Ala Tyr Ser Ala Lys Ala Ile Lys 
        595                 600                 605             


Lys Met Leu Pro Leu Met Arg Cys Gly Lys Tyr Trp Asp Val Ser Ser 
    610                 615                 620                 


Ile Asp Asp Thr Thr Lys Glu Arg Ile Lys Lys Ile Val Thr Gly Glu 
625                 630                 635                 640 


Tyr Asp Glu Arg Ile Ala Val Lys Val Arg Asp Lys Val Met Asn Leu 
                645                 650                 655     


Ser Asp Ile Ser Cys Phe Arg Arg Leu Pro Leu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Ile Val Tyr Asn Arg His Ser Glu Asp Lys Glu Ala Val Lys Trp Glu 
        675                 680                 685             


Ser Pro Ala Asp Ile Asp Ala Tyr Leu Ala Ser Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu Arg Val 
705                 710                 715                 720 


Val Arg Asp Ile Trp Lys Gln Val Gly Arg Ile Asp Glu Ile His Ile 
                725                 730                 735     


Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Asp Lys Arg Lys Arg Met 
            740                 745                 750         


Thr Ala Gln Ile Leu Glu Asn Glu Asn Ala Asn Leu Arg Ile Lys Ala 
        755                 760                 765             


Leu Leu Ala Glu Phe Met Asn Pro Glu Tyr Glu Ile Glu Asn Val Arg 
    770                 775                 780                 


Pro Tyr Ser Pro Ser Gln Gln Glu Ile Leu Arg Ile Tyr Glu Asp Thr 
785                 790                 795                 800 


Val Leu His Ala Glu Asp Gln Leu Pro Glu Glu Ile Asp Ala Ile Leu 
                805                 810                 815     


Lys Lys Phe Asn Ser Ser Lys Val Pro Thr Lys Ser Glu Phe Leu Arg 
            820                 825                 830         


Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Glu 
        835                 840                 845             


Leu Ile Pro Leu Gly Lys Leu Phe Thr Ala Ala Tyr Glu Ile Glu His 
    850                 855                 860                 


Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val 
865                 870                 875                 880 


Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp Asn Gln Leu Gly Tyr 
                885                 890                 895     


Glu Phe Ile Lys Asn His His Gly Gln Lys Val Glu Val Gly Phe Gly 
            900                 905                 910         


Lys Ser Val Glu Ile Phe Ser Val Asp Ser Tyr Glu Arg Phe Val Lys 
        915                 920                 925             


Glu Gln Tyr Ala Lys Ser Ala Val Lys Met Lys Lys Leu Leu Met Asp 
    930                 935                 940                 


Asp Ile Pro Glu Gln Phe Ile Glu Arg Gln Leu Asn Asp Ser Arg Tyr 
945                 950                 955                 960 


Ile Ser Lys Val Ile Lys Gly Leu Leu Ser Asn Ile Val Arg Glu Lys 
                965                 970                 975     


Asn Asp Ser Gly Glu Tyr Glu Pro Glu Ala Val Ser Lys Asn Ile Val 
            980                 985                 990         


Val Cys Thr Gly Ser Val Thr Asp  Arg Leu Lys Arg Asp  Trp Gly Met 
        995                 1000                 1005             


Asn Asp  Val Trp Asn Ser Ile  Val Tyr Pro Arg Phe  Glu Arg Leu 
    1010                 1015                 1020             


Asn Ser  Leu Thr Asp Thr Gln  Ser Phe Gly His Trp  Glu Asn Lys 
    1025                 1030                 1035             


Ser Gly  Lys Lys Val Phe Gln  Thr Glu Leu Pro Leu  Glu Tyr Gln 
    1040                 1045                 1050             


Lys Gly  Phe Ser Lys Lys Arg  Ile Asp His Arg His  His Ala Met 
    1055                 1060                 1065             


Asp Ala  Ile Val Ile Ala Cys  Ala Thr Arg Asn His  Val Asn Tyr 
    1070                 1075                 1080             


Leu Ser  Asn Glu Ser Ala Ser  Arg Asn Ala Lys Ile  Ser Arg Tyr 
    1085                 1090                 1095             


Asp Leu  Gln Arg Leu Leu Cys  Asp Lys Asn Arg Thr  Asp Asp Asn 
    1100                 1105                 1110             


Gly Asn  Tyr Lys Trp Val Ile  Lys Lys Pro Trp Asp  Thr Phe Thr 
    1115                 1120                 1125             


Gln Asp  Ala Arg Glu Val Leu  Ser Lys Ile Val Val  Ser Leu Lys 
    1130                 1135                 1140             


Gln Asn  Leu Arg Ile Ile Asn  Lys Thr Thr Asn Val  Tyr Arg His 
    1145                 1150                 1155             


Phe Asp  Ala Thr Val Asn Arg  Val Tyr Glu Lys Gln  Lys Thr Gly 
    1160                 1165                 1170             


Asp Ser  Trp Ala Ile Arg Lys  Pro Met His Lys Asp  Thr Val Phe 
    1175                 1180                 1185             


Gly Ala  Val Asn Leu Arg Lys  Val Lys Val Val Arg  Leu Ser Val 
    1190                 1195                 1200             


Ala Leu  Asp Thr Pro Ala Met  Ile Val Asp Lys Arg  Val Lys Asn 
    1205                 1210                 1215             


Lys Val  Phe Glu Leu Leu Ser  Tyr Lys Tyr Asp Lys  Lys Arg Ile 
    1220                 1225                 1230             


Glu Lys  Tyr Phe Lys Glu Asn  Ala Phe Leu Trp Lys  Glu Leu Asp 
    1235                 1240                 1245             


Leu Ala  Lys Val Ala Val Tyr  Tyr Phe Thr Glu Ala  Thr Thr Glu 
    1250                 1255                 1260             


Pro Leu  Val Ala Val Arg Lys  Pro Leu Asp Ser Thr  Phe Asn Glu 
    1265                 1270                 1275             


Lys Lys  Ile Lys Glu Ser Val  Thr Asp Thr Gly Ile  Gln Lys Ile 
    1280                 1285                 1290             


Leu Leu  Asn His Leu Cys Thr  Lys Glu Gly Lys Pro  Asp Leu Ala 
    1295                 1300                 1305             


Phe Ser  Ala Glu Gly Ile Glu  Glu Met Asn Arg Asn  Leu Leu Gln 
    1310                 1315                 1320             


Leu Asn  Asn Gly Lys Ser His  Gln Pro Ile Tyr Lys  Val Arg Val 
    1325                 1330                 1335             


Tyr Glu  Pro Arg Gly Asn Lys  Phe Ser Val Gly Val  Leu Gly Asn 
    1340                 1345                 1350             


Lys Gly  Thr Lys Trp Val Glu  Ala Ala Lys Gly Thr  Asn Leu Phe 
    1355                 1360                 1365             


Phe Ala  Ile Tyr Ile Thr Glu  Asp Gly Lys Arg Thr  Tyr Glu Thr 
    1370                 1375                 1380             


Ile Pro  Leu Asn Val Val Ile  Glu Arg Glu Lys Gln  Gly Leu Val 
    1385                 1390                 1395             


Pro Val  Pro Asp Arg Asn Glu  Lys Gly Asp Lys Leu  Leu Phe Trp 
    1400                 1405                 1410             


Leu Ser  Pro Asn Asp Leu Val  Tyr Leu Pro Thr Glu  Glu Glu Arg 
    1415                 1420                 1425             


Glu Phe  Gly Arg Ile Asn Glu  Pro Ile Asp Arg Gly  Arg Ile Tyr 
    1430                 1435                 1440             


Lys Met  Val Ser Cys Thr Gly  Asn Glu Gly His Phe  Ile Pro Ala 
    1445                 1450                 1455             


Asn Val  Ala Asn Pro Ile Val  Pro Val Ile Glu Leu  Gly Ser Asn 
    1460                 1465                 1470             


Asn Lys  Ala Gln Arg Ala Trp  Thr Asn Glu Met Ile  Lys Asp Ile 
    1475                 1480                 1485             


Cys Ile  Pro Ile Lys Val Asp  Arg Leu Gly His Ile  Ile Glu Val 
    1490                 1495                 1500             


Lys Tyr  Arg Ile Asp Glu 
    1505                 


<210>  1316
<211>  1509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1316

Met Lys Lys Thr Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Ser Asn Ile Asp Ser Glu Gly Lys Glu Lys Leu Val 
            20                  25                  30          


Gly Ile Ser Ser Cys Gly Ser Arg Ile Ile Pro Met Asp Ala Thr Thr 
        35                  40                  45              


Leu Gly Asp Phe Gly Lys Gly Asn Thr Lys Ser Pro Val Ala Glu Arg 
    50                  55                  60                  


Thr Arg Leu Arg Gly Ile Arg Arg Leu Leu Glu Arg Ser Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Val Leu Ser Val Met Gly Phe Leu Pro Glu 
                85                  90                  95      


His Tyr Ala Ser Gln Leu Asp Arg Tyr Gly Lys Phe Leu Pro Glu Thr 
            100                 105                 110         


Glu Pro Lys Leu Ala Trp Tyr Lys Asp Asp Ser Gly Arg Tyr Gln Phe 
        115                 120                 125             


Leu Phe Gln Lys Ser Phe His Glu Met Leu Glu Asp Phe Arg Gln His 
    130                 135                 140                 


Gln Pro Glu Leu Val Ala Gly Glu Lys Lys Ile Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Glu Ile Thr Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Gln Glu Glu Asn Asn Lys Ser Val Glu 
        195                 200                 205             


Tyr His Ala Leu Lys Val Val Ser Val Glu Asp Ser Gly Glu Arg Lys 
    210                 215                 220                 


Gly Lys Asp Ile Trp Tyr Asn Val Thr Leu Glu Asn Gly Trp Val Tyr 
225                 230                 235                 240 


Arg Arg Ala Ser Asn Ile Pro Leu Asp Trp Thr Gly Lys Val Lys Glu 
                245                 250                 255     


Phe Val Val Thr Thr Glu Leu Asp Asp Ala Gly Asn Pro Lys Lys Asp 
            260                 265                 270         


Lys Glu Gly Asn Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp Asp 
        275                 280                 285             


Trp Gly Leu Leu Lys Thr Arg Thr Gln Ala Gln Ile Asp Glu Ser Gly 
    290                 295                 300                 


Lys Thr Val Gly Thr Tyr Ile Tyr Glu Ser Leu Leu Cys Met Pro Asn 
305                 310                 315                 320 


Gln Lys Ile Arg Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr 
                325                 330                 335     


Lys Asp Glu Leu Arg Gln Ile Leu Val Lys Gln Ser Glu Phe His Ala 
            340                 345                 350         


Ala Leu Gln Asp His Asn Leu Leu Leu Ser Cys Ile Glu Glu Leu Tyr 
        355                 360                 365             


Pro Asn Asn Glu Ala His Arg Arg Leu Leu Ser Ala Ser Ser Phe Ile 
    370                 375                 380                 


Tyr Phe Leu Ile Glu Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys Ser 
385                 390                 395                 400 


Gln Lys Gly Leu Ile Asp Asn Cys Pro Tyr Glu Ser His Ile Tyr Lys 
                405                 410                 415     


Asp Lys Lys Asp Gly Ser Leu His His Val Pro Leu Lys Cys Val Ser 
            420                 425                 430         


Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Leu Ser 
        435                 440                 445             


Asn Leu Arg Ile Tyr Gln Arg Glu Arg Met Met Asp Gly Ser Leu Lys 
    450                 455                 460                 


Leu Asp Val Asp Val Thr Arg Glu Phe Leu Pro Ser Glu Glu Asp Tyr 
465                 470                 475                 480 


Val Lys Leu Phe Asp Trp Leu Asn Glu Arg Lys Glu Ile Ser Gln Lys 
                485                 490                 495     


Phe Leu Leu Ala Tyr Lys Pro Phe Gly Leu Lys Lys Asn Glu Gln Ala 
            500                 505                 510         


Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys Ser Tyr Pro Cys Asn Glu 
        515                 520                 525             


Thr Arg Ala Glu Ile Lys Ser Arg Leu Ser Lys Ala Gly Val Pro Glu 
    530                 535                 540                 


Glu Phe Leu Thr Glu Glu Lys Glu Glu Ala Leu Trp His Ile Leu Tyr 
545                 550                 555                 560 


Ser Ile Ser Asp Lys Lys Glu Leu Thr Lys Ala Leu Gly Thr Phe Ala 
                565                 570                 575     


Ala Lys Asn Cys Leu Asn Glu Ser Phe Val Glu Val Phe Ala Lys Ile 
            580                 585                 590         


Pro Pro Phe Glu Ser Asn Tyr Ala Ala Tyr Ser Leu Lys Ala Ile Arg 
        595                 600                 605             


Lys Leu Leu Ala Leu Met Arg Met Gly Lys Tyr Trp Asn Glu Gln Ala 
    610                 615                 620                 


Ile Asp Arg Gln Thr Arg Asp Arg Ile Glu Lys Ile Leu Thr Gly Glu 
625                 630                 635                 640 


Tyr Asp Glu Thr Ile Arg Ser Arg Val Arg Glu Lys Ala Met Leu Leu 
                645                 650                 655     


Thr Asp Ile Ser Ser Phe Arg Gly Leu Pro Leu Trp Leu Ala Cys Tyr 
            660                 665                 670         


Ile Val Tyr Asp Arg His Ser Glu Ser Lys Glu Leu Val Lys Trp Glu 
        675                 680                 685             


Thr Pro Ala Asp Ile Asp His Phe Leu Ser Lys Phe Lys Gln Asn Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Ser Leu Arg Thr 
705                 710                 715                 720 


Val Arg Asp Ile Trp Lys Gln Glu Gly Lys Ile Asp Glu Ile His Val 
                725                 730                 735     


Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Lys Glu Arg Ala Arg Ile 
            740                 745                 750         


Thr Ala Gln Val Gln Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala 
        755                 760                 765             


Leu Leu Ala Glu Phe Met Asn Pro Glu Phe Glu Ile Glu Asn Val His 
    770                 775                 780                 


Pro Tyr Ser Pro Gly Gln Gln Glu Ile Leu Arg Ile Tyr Glu Asp Gly 
785                 790                 795                 800 


Val Leu Ser Gly Ile Ala Glu Lys Asp Leu Pro Asp Asp Ile Thr Ala 
                805                 810                 815     


Ile Leu Lys Lys Phe Arg Glu Asn Asp Val Lys Lys Arg Pro Thr Thr 
            820                 825                 830         


Ser Glu Val Leu Arg Tyr Lys Leu Trp Leu Glu Gln Arg Tyr Arg Ser 
        835                 840                 845             


Pro Tyr Thr Gly Arg Val Ile Pro Leu Gly Lys Leu Phe Thr Pro Ala 
    850                 855                 860                 


Tyr Glu Ile Glu His Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser 
865                 870                 875                 880 


Ile Ser Asn Lys Val Ile Cys Glu Ser Ala Val Asn Lys Leu Lys Asp 
                885                 890                 895     


Asn Cys Leu Gly Tyr Glu Phe Ile Lys Lys His Ser Gly Glu Met Val 
            900                 905                 910         


Glu Leu Gly Asn Gly Glu Thr Val Pro Val Phe Ser Val Glu Glu Tyr 
        915                 920                 925             


Glu Arg Phe Val Lys Glu Ser Tyr Phe Gly Asn Ser Lys Lys Met Lys 
    930                 935                 940                 


Lys Leu Leu Leu Glu Asp Ile Pro Asp Ser Phe Ile Glu Arg Gln Leu 
945                 950                 955                 960 


Asn Asp Ser Arg Tyr Ile Ser Arg Val Val Thr Ser Leu Leu Ser Asn 
                965                 970                 975     


Leu Val Cys Glu Glu Gly Glu Gln Asp Gly Leu Ser Lys Asn Val Ile 
            980                 985                 990         


Val Cys Thr Gly Gly Ile Thr Asp  Arg Leu Lys Lys Asp  Trp Gly Val 
        995                 1000                 1005             


Gln Glu  Val Trp Asn Arg Ile  Ile Leu Pro Arg Phe  Leu Arg Leu 
    1010                 1015                 1020             


Asn Glu  Ile Thr Gly Arg Thr  Asp Phe Thr Ser Thr  Ser Val Asn 
    1025                 1030                 1035             


Gly His  Leu Leu Pro Ala Leu  Pro Leu Tyr Leu Gln  Lys Gly Phe 
    1040                 1045                 1050             


Asn Lys  Lys Arg Ile Asp His  Arg His His Ala Met  Asp Ala Ile 
    1055                 1060                 1065             


Val Ile  Ala Cys Ala Asn Arg  Asn Ile Val Asn Tyr  Leu Asn Asn 
    1070                 1075                 1080             


Ser Ser  Ala Arg Lys Asn Ser  Glu Ile Ser Arg Tyr  Asp Leu Gln 
    1085                 1090                 1095             


Arg Leu  Leu Cys Glu Lys Val  Lys Thr Asp Ala Asn  Gly Asn Tyr 
    1100                 1105                 1110             


Lys Trp  Ile Leu Arg Lys Pro  Trp Glu Thr Phe Thr  Gln Asp Val 
    1115                 1120                 1125             


Tyr Ala  Ala Leu Thr Asn Ile  Val Val Ser Phe Lys  Gln Asn Leu 
    1130                 1135                 1140             


Arg Val  Ile Asn Arg Thr Thr  Asn Tyr Tyr Gln His  Tyr Asn Glu 
    1145                 1150                 1155             


Ala Gly  Glu Lys Arg Met Ile  Pro Gln Thr Lys Gly  Asp Arg Trp 
    1160                 1165                 1170             


Ala Ile  Arg Lys Pro Met His  Lys Asp Thr Val Tyr  Gly Glu Val 
    1175                 1180                 1185             


Asn Leu  Arg Lys Glu Lys Thr  Leu Pro Leu Arg Asp  Val Val Lys 
    1190                 1195                 1200             


Asn Pro  Ser Ile Val Val Asp  Lys Ser Leu Lys Asn  Lys Leu Tyr 
    1205                 1210                 1215             


Glu Leu  Leu Lys Ser Gln Tyr  Asp Leu Lys Ala Ile  Ala Lys Tyr 
    1220                 1225                 1230             


Phe Glu  Thr His Gln Asp Val  Trp Ala Asp Val Asn  Leu Lys Lys 
    1235                 1240                 1245             


Ile Lys  Val Tyr Tyr Phe Thr  Lys Glu Thr Asn Glu  Arg Phe Phe 
    1250                 1255                 1260             


Ala Thr  Arg Lys Ser Leu Asp  Pro Ser Phe Asp Gln  Lys Lys Ile 
    1265                 1270                 1275             


Glu Glu  Glu Val Thr Asp Thr  Gly Val Gln Lys Ile  Leu Leu His 
    1280                 1285                 1290             


His Leu  Gln Gln Asn Asn Asn  Asp Pro Asp Met Ala  Phe Ser Pro 
    1295                 1300                 1305             


Asp Gly  Ile Asp Arg Met Asn  Gln Asn Met Thr Ile  Leu Asn Asp 
    1310                 1315                 1320             


Gly Lys  Trp His Gln Pro Ile  Tyr Lys Val Arg Thr  Tyr Glu Lys 
    1325                 1330                 1335             


Ala Asp  Lys Phe Ala Val Gly  Glu Ser Gly Asn Lys  Ala Lys Lys 
    1340                 1345                 1350             


Phe Val  Glu Ala Ala Lys Gly  Thr Asn Leu Phe Phe  Ala Val Tyr 
    1355                 1360                 1365             


Glu Ser  Val Gln Glu Asp Glu  Ala Ile Gly Lys Gln  Val Cys Lys 
    1370                 1375                 1380             


Arg Thr  Phe Ala Thr Ile Pro  Leu Asn Glu Val Ile  Lys Arg Lys 
    1385                 1390                 1395             


Lys Gln  Gly Leu Pro Ala Ala  Pro Glu Asp Leu Asn  Gly Asn Leu 
    1400                 1405                 1410             


Pro Lys  Phe Val Leu Ser Pro  Asn Asp Leu Val Tyr  Leu Pro Thr 
    1415                 1420                 1425             


Glu Glu  Glu Arg Asn Ser Ser  Arg Ile Ile Gln Pro  Leu Asp Arg 
    1430                 1435                 1440             


Glu Arg  Ile Tyr Lys Met Val  Ser Ser Ser Gly Ser  Gln Cys Phe 
    1445                 1450                 1455             


Phe Ile  Lys Val Phe Val Ala  Asn Ser Ile Trp Asp  Lys Asn Glu 
    1460                 1465                 1470             


Tyr Ser  Ser Leu Asn Lys Met  Glu Arg Ala Ile Thr  Asn Glu Met 
    1475                 1480                 1485             


Ile Lys  Glu Ile Cys Val Pro  Ile Lys Ile Asp Arg  Leu Gly Asn 
    1490                 1495                 1500             


Val Ser  Leu Ile Gln Ile 
    1505                 


<210>  1317
<211>  1510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1317

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asp Ser Glu Glu Gln Arg Ile Leu Gly Met Gly Ser Arg 
            20                  25                  30          


Ile Ile Pro Met Asp Gln Gly Val Leu Asp Thr Phe Ser Gly Gly Asn 
        35                  40                  45              


Pro Val Glu Thr Gln Thr Ala Ala Arg Thr Val Tyr Arg Gly Thr Arg 
    50                  55                  60                  


Arg Leu Arg Glu Arg Ala Leu Leu Arg Arg Glu Arg Leu Ile Arg Val 
65                  70                  75                  80  


Leu Asn Met Met Lys Phe Leu Pro Glu His Tyr Ala Ser Gln Ile Asp 
                85                  90                  95      


Phe Glu Lys Arg Leu Gly Gln Phe Phe Asp Glu Lys Glu Pro Lys Leu 
            100                 105                 110         


Ala Trp Lys Gln Asn Asp Glu Gly Arg Phe Glu Phe Ile Phe Gln Gln 
        115                 120                 125             


Ser Phe Asn Glu Met Ile Glu Glu Phe Lys Asn Lys Gly Val Val Arg 
    130                 135                 140                 


Glu Asp Lys Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys 
145                 150                 155                 160 


Lys Ala Leu Ser Gln Glu Ile Glu Lys Glu Glu Leu Ala Trp Ile Leu 
                165                 170                 175     


Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu 
            180                 185                 190         


Glu Glu Glu Asn Pro Asn Lys Leu Val Glu Tyr Tyr Ser Leu Lys Ile 
        195                 200                 205             


Val Asp Val Ile Ala Asp Glu Lys Pro Asn Ser Lys Ser Asp Ile Trp 
    210                 215                 220                 


Tyr Ser Leu His Leu Glu Asn Gly Trp Ile Tyr Arg Arg Ser Ser Lys 
225                 230                 235                 240 


Ile Pro Leu Phe Gly Trp Lys Asp Lys Thr Arg Asp Phe Ile Val Thr 
                245                 250                 255     


Thr Asp Leu Asn Asp Asp Gly Thr Val Lys Lys Asp Lys Glu Gly Ile 
            260                 265                 270         


Glu Lys Arg Ser Phe Arg Ala Pro Ser Glu Asn Asp Trp Val Leu Leu 
        275                 280                 285             


Lys Lys Lys Thr Glu Ser Asp Ile Glu Lys Ser His Lys Thr Val Gly 
    290                 295                 300                 


Ala Tyr Val Tyr Glu Thr Leu Leu Gln Asn Pro Met Gln Lys Val Arg 
305                 310                 315                 320 


Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Asp Glu Leu 
                325                 330                 335     


Lys Gln Ile Leu Glu Lys Gln Arg Glu Phe His Pro Glu Leu Arg Asp 
            340                 345                 350         


Glu Asp Leu Tyr Asn Asp Cys Val Arg Glu Leu Tyr Arg Ser Asn Glu 
        355                 360                 365             


Thr Tyr Gln Phe Ile Leu Ser Lys Arg Asp Phe Val His Leu Phe Leu 
    370                 375                 380                 


Asp Asp Ile Leu Phe Tyr Gln Arg Pro Leu Arg Ser Gln Lys Ser Ala 
385                 390                 395                 400 


Ile Gly Asp Cys Ile Leu Glu Phe Lys Thr Tyr Lys Asp Lys Asp Gly 
                405                 410                 415     


Asn Asn Ile Lys Glu Tyr Leu Lys Val Ile Ser Lys Ser Asn Pro Leu 
            420                 425                 430         


Tyr Gln Glu Phe Arg Ile Trp Gln Trp Leu Tyr Asn Leu Lys Ile Tyr 
        435                 440                 445             


Arg Lys Glu Asp Asp Glu Asp Val Thr Leu Gln Phe Ile Ala Asn Ile 
    450                 455                 460                 


Glu Asp Lys Glu Gln Leu Phe Asp Phe Leu Ser Asn Arg Lys Ser Ile 
465                 470                 475                 480 


Glu Gln Lys Pro Leu Leu Glu Tyr Leu Ile Lys Ala Lys Gly Leu Lys 
                485                 490                 495     


Lys Gln Val Lys Thr Glu Ala Tyr Arg Trp Asn Tyr Val Glu Asp Lys 
            500                 505                 510         


Ile Tyr Pro Cys Ser Glu Thr Lys Thr Met Ile Ser Thr Arg Leu Ala 
        515                 520                 525             


Lys Val Glu Asn Ile Pro Asp Asn Phe Phe Thr Lys Glu Ile Glu Gln 
    530                 535                 540                 


Lys Leu Trp His Ile Ile Tyr Ser Val Thr Asp Lys Asn Glu Phe Glu 
545                 550                 555                 560 


Lys Ala Leu Arg Thr Phe Ala Lys Lys Tyr Asp Leu Asp Ile Ala Ser 
                565                 570                 575     


Phe Val Asp Asn Phe Lys Lys Ile Pro Pro Phe Lys Asn Glu Tyr Gly 
            580                 585                 590         


Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Leu 
        595                 600                 605             


Gly Lys Tyr Trp Lys Trp Asn Asp Ile Asp Asn Asn Thr Gln Asn Arg 
    610                 615                 620                 


Ile Ser Lys Ile Ile Thr Gly Glu Tyr Asp Glu Glu Ile Lys Asp Ile 
625                 630                 635                 640 


Val Arg Glu Lys Ser Ile Ala Leu Thr Asn Glu Asn Asp Phe Gln Gly 
                645                 650                 655     


Leu Gln Leu Trp Leu Ala Gln Tyr Leu Val Tyr Gly Arg His Ser Glu 
            660                 665                 670         


Ala Asp Ile Ala Gly Lys Trp His Ser Val Ala Asp Leu Glu Lys Tyr 
        675                 680                 685             


Leu Asn Glu Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln 
    690                 695                 700                 


Ile Ile Thr Glu Thr Leu Arg Val Val Arg Asp Ile Trp Arg Lys Tyr 
705                 710                 715                 720 


Gly Gln Gly Ala Glu Asn Phe Phe Ser Glu Ile His Val Glu Leu Gly 
                725                 730                 735     


Arg Asp Met Lys Asn Thr Ala Asp Glu Arg Lys Lys Ile Val Asn Val 
            740                 745                 750         


Val Thr Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala Leu Leu Met 
        755                 760                 765             


Glu Leu Lys Asn Asn Ser Asp Gly Lys Leu Glu Val Glu Asn Val Arg 
    770                 775                 780                 


Pro Tyr Ser Pro Thr Gln Gln Asp Ile Leu Lys Ile Tyr Glu Glu Tyr 
785                 790                 795                 800 


Ala Ile Ser Thr Gly Leu Asp Asn Glu Lys Asp Glu Lys Val Lys Glu 
                805                 810                 815     


Asp Ile Lys Lys Ile Ser Arg Val Ala Gln Pro Thr Thr Thr Glu Leu 
            820                 825                 830         


Gln Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Cys Ser Pro Tyr Thr 
        835                 840                 845             


Gly Lys Val Ile Pro Leu Gly Lys Leu Phe Thr Glu Glu Tyr Gln Ile 
    850                 855                 860                 


Glu His Ile Ile Pro Lys Ser Arg Tyr Tyr Asp Asp Ser Phe Ser Asn 
865                 870                 875                 880 


Lys Val Ile Cys Glu Ala Ala Val Asn Lys Leu Lys Asp Lys Cys Leu 
                885                 890                 895     


Gly Leu Glu Phe Ile Lys Asn Tyr His Gly Gln Ile Val Glu Thr Gly 
            900                 905                 910         


Phe Gly Gln Lys Val Thr Ile Phe Asp Glu Glu Ala Tyr Gln Asn Phe 
        915                 920                 925             


Val Lys Gln His Tyr Ala Tyr Asn His Ser Lys Arg Thr Lys Leu Leu 
    930                 935                 940                 


Leu Glu Glu Ile Pro Glu Lys Met Ile Glu Arg Gln Met Asn Asp Thr 
945                 950                 955                 960 


Arg Tyr Ile Ser Lys Phe Val Leu Pro Leu Leu Ser Asn Leu Val Arg 
                965                 970                 975     


Ala Lys Glu Asn Asp Asn Gly Val Asn Ser Lys Asn Val Leu Pro Val 
            980                 985                 990         


Asn Gly Lys Ile Thr Thr Met Leu  Lys Gln Asp Trp Gly  Leu Asn Asp 
        995                 1000                 1005             


Val Trp  Asn Asp Leu Ile Leu  Pro Arg Phe Val Arg  Met Asn Glu 
    1010                 1015                 1020             


Leu Ala  Lys Thr Ile Ala Phe  Thr Ser Trp Asn Glu  Gln His Gln 
    1025                 1030                 1035             


Lys Tyr  Leu Pro Thr Val Pro  Leu Glu Leu Ser Lys  Gly Phe Gln 
    1040                 1045                 1050             


Lys Lys  Arg Ile Asp His Arg  His His Ala Met Asp  Ala Leu Val 
    1055                 1060                 1065             


Ile Ala  Cys Ala Thr Arg Asp  His Val Asn Leu Leu  Asn Asn Lys 
    1070                 1075                 1080             


His Ala  Asn Thr Asp Thr Ile  Arg Tyr Asp Leu Gln  Arg Lys Leu 
    1085                 1090                 1095             


Arg Leu  Phe Glu Arg Val Thr  Tyr Ile Asp Pro Gln  Thr Lys Asn 
    1100                 1105                 1110             


Asn Val  Thr Lys Asp Ile Pro  Lys Glu Phe Lys Lys  Pro Trp Asp 
    1115                 1120                 1125             


Asn Phe  Thr Val Asp Ala Arg  Asn Glu Leu Glu Lys  Ile Ile Val 
    1130                 1135                 1140             


Ser Phe  Lys Gln Asn Leu Arg  Ile Ile Asn Lys Ala  Thr Asn Ile 
    1145                 1150                 1155             


Tyr Thr  Lys Tyr Glu Asn Gly  Lys Lys Ile Lys Val  Gly Gln Lys 
    1160                 1165                 1170             


Gly Leu  Asn Trp Ala Ile Arg  Lys Pro Leu His Lys  Glu Thr Val 
    1175                 1180                 1185             


Phe Ala  Lys Val Ser Leu Arg  Lys Arg Lys Thr Val  Arg Leu Ser 
    1190                 1195                 1200             


Glu Ala  Leu Lys Asp Trp Lys  Lys Ile Val Asp Lys  Lys Leu Lys 
    1205                 1210                 1215             


Gln Glu  Ile Lys Arg Leu Thr  Cys Gln Tyr Gly Lys  Phe Asp Val 
    1220                 1225                 1230             


Asp Thr  Ile Leu Arg Tyr Phe  Lys Asp Arg Lys Tyr  Gln Phe Gly 
    1235                 1240                 1245             


Glu Val  Asp Val Ser Lys Val  Lys Met Tyr Tyr Phe  Asp Glu Glu 
    1250                 1255                 1260             


Asn Ala  Ala Val Arg Lys Asn  Val Asp Thr Ser Phe  Thr Glu Lys 
    1265                 1270                 1275             


Phe Ile  Gln Gly Ser Val Thr  Asp Thr Gly Ile Gln  Lys Ile Leu 
    1280                 1285                 1290             


Leu Asn  His Leu Glu Ala Lys  Gly Asn Lys Val Glu  Ile Ala Phe 
    1295                 1300                 1305             


Ser Pro  Glu Gly Ile Glu Glu  Met Asn Lys Asn Ile  Ile Gln Leu 
    1310                 1315                 1320             


Asn Glu  Gly Lys Leu His Leu  Pro Ile Phe Lys Val  Arg Val Tyr 
    1325                 1330                 1335             


Glu Thr  Ile Gly Asn Lys Phe  Ser Val Gly Val Lys  Gly Asn Lys 
    1340                 1345                 1350             


Lys Asp  Lys Tyr Val Glu Ala  Ala Lys Gly Thr Asn  Leu Phe Phe 
    1355                 1360                 1365             


Ala Ile  Tyr Val Asp Glu Asn  Gly Ile Arg Ser Tyr  Glu Thr Ile 
    1370                 1375                 1380             


Pro Leu  Asn Ile Val Ile Glu  Arg Leu Lys Gln Gly  Leu Ser Val 
    1385                 1390                 1395             


Val Pro  Glu Lys Asn Arg Lys  Glu His Ser Leu Leu  Phe Tyr Leu 
    1400                 1405                 1410             


Ser Pro  Asn Asp Leu Val Tyr  Val Pro Ile Glu Asp  Glu Arg Glu 
    1415                 1420                 1425             


Asn Ile  His Ala Val Asn Leu  Asp Gln Leu Asn Asn  Glu Gln Arg 
    1430                 1435                 1440             


Lys Arg  Ile Tyr Lys Met Val  Ser Cys Thr Gly Ser  Glu Cys His 
    1445                 1450                 1455             


Phe Val  Pro Tyr Tyr Val Ala  Ser Pro Ile Val Asn  Lys Val Glu 
    1460                 1465                 1470             


Tyr Ser  Ser Leu Asn Lys Ile  Gly Arg Ser Leu Thr  Gly Glu Met 
    1475                 1480                 1485             


Ile Lys  Asp Val Cys Ile Lys  Leu Lys Val Asp Arg  Leu Gly Asn 
    1490                 1495                 1500             


Ile Lys  Gly Ile Ser Gly Leu  
    1505                 1510 


<210>  1318
<211>  1510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1318

Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asp Ser Glu Glu Gln Arg Ile Leu Gly Met Gly Ser Arg 
            20                  25                  30          


Ile Ile Pro Met Asp Gln Gly Val Leu Asp Thr Phe Ser Gly Gly Asn 
        35                  40                  45              


Pro Val Glu Thr Gln Thr Ala Ala Arg Thr Ala Tyr Arg Gly Thr Arg 
    50                  55                  60                  


Arg Leu Arg Glu Arg Ala Leu Leu Arg Arg Glu Arg Leu Ile Arg Val 
65                  70                  75                  80  


Leu Asn Met Met Lys Phe Leu Pro Glu His Tyr Ala Ser Gln Ile Asp 
                85                  90                  95      


Phe Glu Lys Arg Leu Gly Gln Phe Phe Asp Glu Lys Glu Pro Lys Leu 
            100                 105                 110         


Ala Trp Lys Gln Asn Asp Glu Gly Arg Phe Glu Phe Ile Phe Gln Gln 
        115                 120                 125             


Ser Phe Asn Glu Met Ile Glu Glu Phe Lys Asn Lys Gly Val Val Arg 
    130                 135                 140                 


Glu Asp Lys Lys Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys 
145                 150                 155                 160 


Lys Ala Leu Ser Gln Glu Ile Glu Lys Glu Glu Leu Ala Trp Ile Leu 
                165                 170                 175     


Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu 
            180                 185                 190         


Glu Glu Glu Asn Pro Asn Lys Leu Val Glu Tyr Tyr Ser Leu Lys Ile 
        195                 200                 205             


Val Asp Val Ile Ala Asp Glu Lys Pro Asn Ser Lys Gly Asp Ile Trp 
    210                 215                 220                 


Tyr Ser Leu His Leu Glu Asn Gly Trp Ile Tyr Arg Arg Ser Ser Lys 
225                 230                 235                 240 


Ile Pro Leu Phe Gly Trp Lys Asp Lys Thr Arg Asp Phe Ile Val Thr 
                245                 250                 255     


Thr Asp Leu Asn Asp Asp Gly Thr Val Lys Lys Asp Lys Glu Gly Ile 
            260                 265                 270         


Glu Lys Arg Ser Phe Arg Ala Pro Ser Glu Asn Asp Trp Val Leu Leu 
        275                 280                 285             


Lys Lys Lys Thr Glu Ser Asp Ile Glu Lys Ser His Lys Thr Val Gly 
    290                 295                 300                 


Ala Tyr Val Tyr Glu Thr Leu Leu Gln Asn Pro Met Gln Lys Val Arg 
305                 310                 315                 320 


Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Asp Glu Leu 
                325                 330                 335     


Lys Gln Ile Leu Glu Lys Gln Arg Glu Phe His Pro Glu Leu Arg Asp 
            340                 345                 350         


Glu Asp Leu Tyr Asn Asp Cys Val Arg Glu Leu Tyr Arg Ser Asn Glu 
        355                 360                 365             


Thr Tyr Gln Phe Ile Leu Ser Lys Arg Asp Phe Val His Leu Phe Leu 
    370                 375                 380                 


Asp Asp Ile Leu Phe Tyr Gln Arg Pro Leu Arg Ser Gln Lys Ser Ala 
385                 390                 395                 400 


Ile Gly Asp Cys Ile Leu Glu Phe Lys Thr Tyr Lys Asp Lys Asp Gly 
                405                 410                 415     


Asn Asn Ile Lys Glu Tyr Leu Lys Val Ile Ser Lys Ser Asn Pro Leu 
            420                 425                 430         


Tyr Gln Glu Phe Arg Ile Trp Gln Trp Leu Tyr Asn Leu Lys Ile Tyr 
        435                 440                 445             


Arg Lys Glu Asp Asp Glu Asp Val Thr Leu Gln Phe Ile Ala Asn Ile 
    450                 455                 460                 


Glu Asp Lys Glu Gln Leu Phe Asp Phe Leu Ser Asn Arg Lys Ser Ile 
465                 470                 475                 480 


Glu Gln Lys Pro Leu Leu Glu Tyr Leu Ile Lys Ala Lys Gly Leu Lys 
                485                 490                 495     


Lys Gln Val Lys Thr Glu Ala Tyr Arg Trp Asn Tyr Val Glu Asp Lys 
            500                 505                 510         


Ile Tyr Pro Cys Ser Glu Thr Lys Thr Met Ile Ser Thr Arg Leu Ala 
        515                 520                 525             


Lys Val Glu Asn Ile Pro Asp Asn Phe Phe Thr Lys Glu Ile Glu Gln 
    530                 535                 540                 


Lys Leu Trp His Ile Ile Tyr Ser Val Thr Asp Lys Asn Glu Phe Glu 
545                 550                 555                 560 


Lys Ala Leu Arg Thr Phe Ala Lys Lys Tyr Asp Leu Asp Ile Ala Ser 
                565                 570                 575     


Phe Val Asp Asn Phe Lys Lys Ile Pro Pro Phe Lys Asn Glu Tyr Gly 
            580                 585                 590         


Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Leu 
        595                 600                 605             


Gly Lys Tyr Trp Lys Trp Asn Asp Ile Asp Asn Asn Thr Gln Asn Arg 
    610                 615                 620                 


Ile Ser Lys Ile Ile Thr Gly Glu Tyr Asp Glu Glu Ile Lys Asp Ile 
625                 630                 635                 640 


Val Arg Glu Lys Ser Ile Ala Leu Thr Asn Glu Asn Asp Phe Gln Gly 
                645                 650                 655     


Leu Gln Leu Trp Leu Ala Gln Tyr Leu Val Tyr Gly Arg His Ser Glu 
            660                 665                 670         


Ala Asp Ile Ala Gly Lys Trp His Ser Val Ala Asp Leu Glu Lys Tyr 
        675                 680                 685             


Leu Asn Glu Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln 
    690                 695                 700                 


Ile Ile Thr Glu Thr Leu Arg Val Val Arg Asp Ile Trp Arg Lys Tyr 
705                 710                 715                 720 


Gly Gln Gly Ala Glu Asn Phe Phe Ser Glu Ile His Val Glu Leu Gly 
                725                 730                 735     


Arg Asp Met Lys Asn Thr Ala Asp Glu Arg Lys Lys Ile Val Asn Val 
            740                 745                 750         


Val Thr Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala Leu Leu Met 
        755                 760                 765             


Glu Leu Lys Asn Asn Ser Asp Gly Lys Leu Glu Val Glu Asn Val Arg 
    770                 775                 780                 


Pro Tyr Ser Pro Thr Gln Gln Asp Ile Leu Lys Ile Tyr Glu Glu Tyr 
785                 790                 795                 800 


Ala Ile Ser Thr Gly Leu Asp Asn Glu Lys Asp Glu Lys Val Lys Glu 
                805                 810                 815     


Asp Ile Lys Lys Ile Ser Arg Val Ala Gln Pro Thr Thr Thr Glu Leu 
            820                 825                 830         


Gln Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Cys Ser Pro Tyr Thr 
        835                 840                 845             


Gly Lys Val Ile Pro Leu Gly Lys Leu Phe Thr Glu Glu Tyr Gln Ile 
    850                 855                 860                 


Glu His Ile Ile Pro Lys Ser Arg Tyr Tyr Asp Asp Ser Phe Ser Asn 
865                 870                 875                 880 


Lys Val Ile Cys Glu Ala Ala Val Asn Lys Leu Lys Asp Lys Cys Leu 
                885                 890                 895     


Gly Leu Glu Phe Ile Lys Asn Tyr His Gly Gln Ile Val Glu Thr Gly 
            900                 905                 910         


Phe Gly Gln Lys Val Thr Ile Phe Asp Glu Glu Ala Tyr Gln Asn Phe 
        915                 920                 925             


Val Lys Gln His Tyr Ala Tyr Asn Arg Ser Lys Arg Thr Lys Leu Leu 
    930                 935                 940                 


Leu Glu Glu Ile Pro Glu Lys Met Ile Glu Arg Gln Met Asn Asp Thr 
945                 950                 955                 960 


Arg Tyr Ile Ser Lys Phe Val Leu Pro Leu Leu Ser Asn Leu Val Arg 
                965                 970                 975     


Ala Glu Glu Asn Asp Asn Gly Val Asn Ser Lys Asn Val Leu Pro Val 
            980                 985                 990         


Asn Gly Lys Ile Thr Thr Met Leu  Lys Gln Asp Trp Gly  Leu Asn Asp 
        995                 1000                 1005             


Val Trp  Asn Asp Leu Ile Leu  Pro Arg Phe Val Arg  Met Asn Glu 
    1010                 1015                 1020             


Leu Ala  Lys Thr Ile Ala Phe  Thr Ser Trp Asn Glu  Gln His Gln 
    1025                 1030                 1035             


Lys Tyr  Leu Pro Thr Val Pro  Leu Glu Leu Ser Lys  Gly Phe Gln 
    1040                 1045                 1050             


Lys Lys  Arg Ile Asp His Arg  His His Ala Met Asp  Ala Leu Val 
    1055                 1060                 1065             


Ile Ala  Cys Ala Thr Arg Asp  His Val Asn Leu Leu  Asn Asn Lys 
    1070                 1075                 1080             


His Ala  Asn Thr Asp Thr Ile  Arg Tyr Asp Leu Gln  Arg Lys Leu 
    1085                 1090                 1095             


Arg Leu  Phe Glu Arg Val Thr  Tyr Ile Asp Pro Gln  Thr Lys Asn 
    1100                 1105                 1110             


Asn Val  Thr Lys Asp Ile Pro  Lys Glu Phe Lys Lys  Pro Trp Asp 
    1115                 1120                 1125             


Asn Phe  Thr Val Asp Ala Arg  Asn Glu Leu Glu Lys  Ile Ile Val 
    1130                 1135                 1140             


Ser Phe  Lys Gln Asn Leu Arg  Ile Ile Asn Lys Ala  Thr Asn Ile 
    1145                 1150                 1155             


Tyr Thr  Lys Tyr Glu Asn Gly  Lys Lys Ile Lys Val  Gly Gln Lys 
    1160                 1165                 1170             


Gly Leu  Asn Trp Ala Ile Arg  Lys Pro Leu His Lys  Glu Thr Val 
    1175                 1180                 1185             


Phe Ala  Lys Val Ser Leu Arg  Lys Arg Lys Thr Val  Arg Leu Ser 
    1190                 1195                 1200             


Glu Ala  Leu Lys Asp Trp Lys  Lys Ile Val Asp Lys  Lys Leu Lys 
    1205                 1210                 1215             


Gln Glu  Ile Lys Arg Leu Thr  Cys Gln Tyr Gly Lys  Phe Asp Val 
    1220                 1225                 1230             


Asp Thr  Ile Leu Arg Tyr Phe  Lys Asp Arg Lys Tyr  Gln Phe Gly 
    1235                 1240                 1245             


Glu Val  Asp Val Ser Lys Val  Lys Met Tyr Tyr Phe  Asp Glu Glu 
    1250                 1255                 1260             


Asn Ala  Ala Val Arg Lys Asn  Val Asp Thr Ser Phe  Thr Glu Lys 
    1265                 1270                 1275             


Phe Ile  Gln Gly Ser Val Thr  Asp Thr Gly Ile Gln  Lys Ile Leu 
    1280                 1285                 1290             


Leu Asn  His Leu Glu Ala Lys  Gly Asn Lys Val Glu  Ile Ala Phe 
    1295                 1300                 1305             


Ser Pro  Glu Gly Ile Glu Glu  Met Asn Lys Asn Ile  Ile Gln Leu 
    1310                 1315                 1320             


Asn Glu  Gly Lys Leu His Leu  Pro Ile Phe Lys Val  Arg Val Tyr 
    1325                 1330                 1335             


Glu Thr  Ile Gly Asn Lys Phe  Ser Val Gly Val Lys  Gly Asn Lys 
    1340                 1345                 1350             


Lys Asp  Lys Tyr Val Glu Ala  Ala Lys Gly Thr Asn  Leu Phe Phe 
    1355                 1360                 1365             


Ala Ile  Tyr Val Asp Glu Asn  Gly Ile Arg Ser Tyr  Glu Thr Ile 
    1370                 1375                 1380             


Pro Leu  Asn Ile Val Ile Glu  Arg Leu Lys Gln Gly  Leu Ser Val 
    1385                 1390                 1395             


Val Pro  Glu Lys Asn Arg Lys  Glu His Ser Leu Leu  Phe Tyr Leu 
    1400                 1405                 1410             


Ser Pro  Asn Asp Leu Val Tyr  Val Pro Ile Glu Asp  Glu Arg Glu 
    1415                 1420                 1425             


Asn Ile  His Ala Val Asn Leu  Asp Gln Leu Asn Asn  Glu Gln Arg 
    1430                 1435                 1440             


Lys Arg  Ile Tyr Lys Met Val  Ser Cys Thr Gly Ser  Glu Cys His 
    1445                 1450                 1455             


Phe Val  Pro Tyr Tyr Val Ala  Ser Pro Ile Val Asn  Lys Val Glu 
    1460                 1465                 1470             


Tyr Ser  Ser Leu Asn Lys Ile  Gly Arg Ser Leu Thr  Gly Glu Met 
    1475                 1480                 1485             


Ile Lys  Asp Val Cys Ile Lys  Leu Lys Val Asp Arg  Leu Gly Asn 
    1490                 1495                 1500             


Val Lys  Gly Ile Ser Gly Leu  
    1505                 1510 


<210>  1319
<211>  1511
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1319

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Ser Val Ala Asp Glu Ser Val Glu Lys Arg Asn Ile 
            20                  25                  30          


Glu Leu Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Val Leu Gly 
        35                  40                  45              


Asn Phe Asp Asn Gly Val Thr Lys Ser Gln Thr Ala Glu Arg Thr Ser 
    50                  55                  60                  


Tyr Arg Ser Ile Arg Arg Leu Arg Glu Arg Gln Leu Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Lys Val Leu Gln Ile Leu Gly Phe Leu Pro Glu His Tyr 
                85                  90                  95      


Val Asn Asp Ile Asp Phe Glu Asn His Pro Gly Lys Ile Lys Lys Asn 
            100                 105                 110         


Thr Glu Pro Lys Leu Pro Trp Arg Lys Asn Ser Met Gly Gln Phe Glu 
        115                 120                 125             


Phe Ile Phe Gln Ala Ser Phe Glu Ala Met Leu Thr Asp Phe Ala Thr 
    130                 135                 140                 


His Gln Pro Val Phe Val Ser Glu Gly Lys Lys Val His Tyr Asp Trp 
145                 150                 155                 160 


Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Glu Glu Ile Ser Lys 
                165                 170                 175     


Glu Glu Leu Ala Trp Ile Ile Leu Asn Phe Asn Gln Lys Arg Gly Tyr 
            180                 185                 190         


Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu Gln Ser Asn Lys Leu Val 
        195                 200                 205             


Glu Phe Tyr Ala Leu Arg Val Val Lys Val Glu Ala Thr Asp Asp Lys 
    210                 215                 220                 


Lys Gly Lys Asp Thr Trp Tyr Asn Val His Leu Glu Asn Gly Trp Ile 
225                 230                 235                 240 


Tyr Arg Arg Thr Ser Asn Ile Pro Leu Asp Trp Glu Gly Lys Val Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Asp Leu Asn Glu Asp Gly Thr Pro Lys Leu 
            260                 265                 270         


Asp Lys Tyr Gly Glu Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp 
        275                 280                 285             


Asp Trp Thr Leu Ile Lys Lys Lys Thr Glu Ser Asp Ile Ala Asn Ser 
    290                 295                 300                 


His Lys Thr Leu Gly Cys Tyr Ile Tyr Asp Ala Leu Leu Gln Asn Pro 
305                 310                 315                 320 


Asn Gln Lys Ile Lys Gly Arg Leu Val Arg Thr Val Glu Arg Lys Phe 
                325                 330                 335     


Tyr Lys Glu Glu Leu Arg Leu Ile Leu Glu Lys Gln Leu Ser Phe His 
            340                 345                 350         


Pro Glu Leu Gln Asp Arg Asp Leu Tyr Arg Ala Cys Ile Asp Ala Leu 
        355                 360                 365             


Tyr Val Ser Asn Glu Ala His Lys Asn Asn Ile Ser Gly Arg Asp Phe 
    370                 375                 380                 


Val Tyr Leu Phe Ile Asp Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys 
385                 390                 395                 400 


Thr Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Lys Asn Gln Tyr 
                405                 410                 415     


Met Asp Arg Glu Thr Gly Glu Val Lys Gln Ala Pro Ile Lys Cys Ile 
            420                 425                 430         


Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Ile 
        435                 440                 445             


Ser Asn Ile Arg Ile Tyr Gln Arg Glu Arg Val Met Asp Asp Gly Lys 
    450                 455                 460                 


Leu Glu Thr Asp Val Asp Val Thr Pro Asp Phe Leu Lys Thr Glu Asp 
465                 470                 475                 480 


Asp Tyr Thr Thr Leu Phe Glu Trp Leu Asn Asp Arg Lys Glu Val Asp 
                485                 490                 495     


Gln Lys Ala Phe Leu Lys Asn Pro Gln Phe Gly Leu Lys Lys Thr Phe 
            500                 505                 510         


Thr Glu Tyr Arg Trp Asn Tyr Val Glu Asp Lys Ser Tyr Pro Cys Asn 
        515                 520                 525             


Glu Thr Arg Asn Ala Ile Leu Asn Gly Leu Gln Lys Ala Gly Val Ser 
    530                 535                 540                 


Ala Asp Phe Leu Ser Arg Glu Val Glu Glu Ala Leu Trp His Ile Leu 
545                 550                 555                 560 


Tyr Ser Val Glu Asp Lys Ile Glu Ile Glu Ser Ala Leu Arg Lys Phe 
                565                 570                 575     


Ala Ser Lys His Asp Leu Asp Asp Arg Phe Ala Asp Val Phe Lys Lys 
            580                 585                 590         


Leu Pro Pro Phe Arg Lys Glu Tyr Gly Ala Tyr Ser Ala Lys Ala Ile 
        595                 600                 605             


Lys Lys Leu Leu Pro Leu Met Arg Met Gly Lys Tyr Trp Glu Leu Ser 
    610                 615                 620                 


Asn Ile Asp Asp Lys Thr Lys Glu Arg Ile Asp Lys Ile Ile Thr Gly 
625                 630                 635                 640 


Glu Trp Asp Glu Asn Ile His Asn Arg Val Arg Glu Lys Ala Ile Lys 
                645                 650                 655     


Leu Ser Asp Ile Thr Asp Phe Lys Gly Leu Pro Leu Trp Leu Ser Cys 
            660                 665                 670         


Tyr Val Val Tyr Asn Arg His Ser Glu Ala Arg Ala Ile Ala Lys Trp 
        675                 680                 685             


Glu Ser Pro Glu Asp Ile Asp Ser Tyr Leu Arg Leu Phe Arg Gln His 
    690                 695                 700                 


Ser Leu Arg Asn Pro Val Val Glu Gln Val Ile Thr Glu Thr Leu Arg 
705                 710                 715                 720 


Thr Val Arg Asp Ile Trp Lys Arg Ile Gly Gln Ile Asp Glu Ile His 
                725                 730                 735     


Val Glu Leu Gly Arg Glu Ile Lys Asn Pro Ala Asp Lys Arg Ala Lys 
            740                 745                 750         


Met Thr Ala Gln Ile Val Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys 
        755                 760                 765             


Ala Leu Leu Ala Glu Phe Ile Asn Ser Glu Tyr Glu Ile Glu Asn Val 
    770                 775                 780                 


Arg Pro Tyr Ser Pro Gly Gln Gln Glu Ile Leu Arg Ile Tyr Glu Asp 
785                 790                 795                 800 


Thr Val Leu Asn Ser Val Glu Glu Ile Pro Glu Asp Ile Ser Ala Val 
                805                 810                 815     


Leu Lys Lys Phe Arg Glu Ser Asp Leu Lys Lys Arg Pro Thr Lys Ser 
            820                 825                 830         


Glu Phe Leu Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro 
        835                 840                 845             


Tyr Thr Gly Asp Ile Ile Pro Leu Gly Lys Leu Phe Thr Ser Ala Tyr 
    850                 855                 860                 


Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe 
865                 870                 875                 880 


Ser Asn Lys Val Ile Cys Glu Ala Glu Val Asn Lys Leu Lys Ser Asn 
                885                 890                 895     


Glu Leu Gly Tyr Glu Phe Ile Lys Asn His His Gly Gln Tyr Val Glu 
            900                 905                 910         


Ile Gly Phe Gly Lys Val Val Gln Ile Phe Ser Ile Glu Ala Tyr Glu 
        915                 920                 925             


Lys Phe Val Lys Ala Asn Tyr Ser Gly Ile Gly Ser Lys Met Lys Arg 
    930                 935                 940                 


Leu Leu Met Asp Asp Ile Pro Glu Thr Phe Ile Glu Arg Gln Leu Asn 
945                 950                 955                 960 


Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser Leu Leu Ser Asn Ile 
                965                 970                 975     


Val Arg Glu Lys Asp Ser Asn Gly Glu Tyr Glu Pro Glu Ala Ile Ser 
            980                 985                 990         


Lys Asn Val Ile Val Cys Thr Gly  Ser Val Thr Asp Arg  Leu Lys Arg 
        995                 1000                 1005             


Asp Trp  Gly Val Asn Asp Val  Trp Asn Ser Ile Ile  Tyr Pro Arg 
    1010                 1015                 1020             


Phe Glu  Arg Leu Asn Glu Leu  Thr Gly Ser Gln Gln  Phe Gly Cys 
    1025                 1030                 1035             


Trp Glu  Asn Lys Glu Gly Lys  Arg Val Phe Gln Thr  Ala Val Pro 
    1040                 1045                 1050             


Leu Glu  Met Gln Lys Gly Phe  Ser Lys Lys Arg Ile  Asp His Arg 
    1055                 1060                 1065             


His His  Ala Met Asp Ala Ile  Val Ile Ala Cys Ala  Ile Arg Asn 
    1070                 1075                 1080             


His Ile  Asn Tyr Leu Ser Asn  Glu Ser Ala Ser Lys  Lys Ala Lys 
    1085                 1090                 1095             


Ile Ser  Arg Ile Asp Leu Gln  Arg Leu Leu Cys Thr  Lys Cys Arg 
    1100                 1105                 1110             


Thr Asp  Asp Asn Gly Asn Tyr  Lys Trp Ile Val Lys  Lys Pro Trp 
    1115                 1120                 1125             


Pro Ala  Phe Thr Gln Asp Ile  Arg Lys Ala Leu Glu  Asn Ile Thr 
    1130                 1135                 1140             


Val Ser  Phe Lys Gln Asn Leu  Arg Val Ile Asn Lys  Thr Thr Asn 
    1145                 1150                 1155             


Tyr Tyr  Val His Phe Asp Glu  Ser Gly Lys Lys Val  Leu Glu Lys 
    1160                 1165                 1170             


Gln Ile  Asn Gly Asp Ser Trp  Ala Ile Arg Lys Pro  Met His Lys 
    1175                 1180                 1185             


Asp Thr  Val Phe Gly Lys Val  Asn Leu Gln Lys Val  Lys Glu Val 
    1190                 1195                 1200             


Arg Leu  Ser Leu Ala Leu Asp  Thr Pro Lys Met Ile  Val Asp Lys 
    1205                 1210                 1215             


Lys Leu  Lys Glu Lys Val Leu  Gln Leu Tyr Ser Tyr  Lys Tyr Asp 
    1220                 1225                 1230             


Lys Lys  Arg Ile Asp Lys Tyr  Phe Lys Glu Asn Ala  Ser Leu Trp 
    1235                 1240                 1245             


Lys Asp  Leu Asn Leu Ser Lys  Val Ala Val Tyr Tyr  Phe Thr Asn 
    1250                 1255                 1260             


Asp Ser  Thr Glu Pro Leu Val  Ala Val Arg Lys Pro  Leu Asp Thr 
    1265                 1270                 1275             


Ser Phe  Asn Ala Lys Lys Ile  Lys Glu Ser Val Thr  Asp Thr Gly 
    1280                 1285                 1290             


Ile Gln  Lys Ile Leu Leu Asn  His Leu Ser Asn Asn  Gln Asp Asn 
    1295                 1300                 1305             


Pro Asn  Leu Ala Phe Ser Pro  Asp Gly Ile Asp Glu  Met Asn Arg 
    1310                 1315                 1320             


Asn Leu  Thr Thr Leu Asn Asp  Gly Lys Tyr His Gln  Pro Ile Tyr 
    1325                 1330                 1335             


Lys Val  Arg Val Cys Glu Pro  Gln Gly Asn Lys Phe  Ser Val Gly 
    1340                 1345                 1350             


Thr Ser  Gly Asn Lys Thr Ser  Lys Tyr Val Glu Ala  Ala Lys Gly 
    1355                 1360                 1365             


Thr Asn  Leu Phe Phe Ala Val  Tyr Gln Thr Glu Asp  Gly Lys Arg 
    1370                 1375                 1380             


Asn Tyr  Glu Thr Ile Pro Leu  Asn Val Val Ile Glu  Arg Glu Lys 
    1385                 1390                 1395             


Leu Glu  Trp Ala Pro Val Pro  Glu Asn Asn Asn Asn  Gly Asp Lys 
    1400                 1405                 1410             


Leu Leu  Phe Trp Leu Ser Pro  Asn Asp Leu Val Tyr  Val Pro Thr 
    1415                 1420                 1425             


Ala Asp  Glu Leu Asn Asn Gly  Met Arg Leu Asp Asn  Met Asp Lys 
    1430                 1435                 1440             


Gly Arg  Ile Tyr Lys Met Ile  Ser Cys Ser Gly Pro  Gln Cys Phe 
    1445                 1450                 1455             


Phe Ile  Pro Gln Asn Val Ser  Asn Pro Ile Ile Pro  Val Ile Glu 
    1460                 1465                 1470             


Leu Gly  Val Asn Asn Lys Ala  Glu Lys Ser Trp Asn  Asn Glu Met 
    1475                 1480                 1485             


Ile Lys  Val Val Cys Val Pro  Ile Arg Val Asp Arg  Leu Gly Cys 
    1490                 1495                 1500             


Ile Thr  Gln Ile Asn Asn Gln  Lys 
    1505                 1510     


<210>  1320
<211>  1512
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1320

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Glu Asn Ser Phe Thr Glu Lys Glu Gly Lys Ile Asn Asp 
            20                  25                  30          


Met Gly Val Arg Ile Ile Pro Met Ser Ala Asp Ile Leu Gly Lys Phe 
        35                  40                  45              


Asp Ala Gly Gln Ser His Ser Gln Thr Ala Glu Arg Thr Gly Tyr Arg 
    50                  55                  60                  


Gly Thr Arg Arg Leu Tyr Gln Arg Asp Asn Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Ile Leu Gly Phe Leu Pro Lys His Tyr Asp Glu 
                85                  90                  95      


His Ile Asp Phe Glu Lys Arg Leu Gly Gln Phe Lys Glu Gly Lys Glu 
            100                 105                 110         


Ile Lys Leu Asn Tyr Arg Cys Asn Val Glu Asn Lys His Glu Phe Ile 
        115                 120                 125             


Phe Ile Ser Ser Phe Asn Glu Met Leu Thr Glu Phe Lys Lys Ala Gln 
    130                 135                 140                 


Pro Glu Leu Phe Tyr Leu Lys Ser Asn Gly Glu Glu Thr Lys Ile Pro 
145                 150                 155                 160 


Tyr Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Gln Pro 
                165                 170                 175     


Leu Thr Lys Gln Glu Leu Ala Trp Ile Ile Leu Asn Phe Asn Gln Lys 
            180                 185                 190         


Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Ile Asp Asp Asp Lys Asn 
        195                 200                 205             


Lys Gln Phe Val Lys Leu Lys Val Lys Glu Val Ile Asn Ser Gly Glu 
    210                 215                 220                 


Ala Val Lys Gly Lys Pro Leu Phe Asp Val Ile Phe Glu Asn Gly Trp 
225                 230                 235                 240 


Lys Tyr Asp Lys Gln Val Val Lys Thr Glu Asp Trp Ile Asp Arg Thr 
                245                 250                 255     


Lys Glu Phe Ile Val Thr Thr Lys Val Leu Lys Asn Gly Asp Leu Lys 
            260                 265                 270         


Arg Ser Tyr Lys Thr Val Asp Ser Glu Lys Asp Trp Ala Ala Ile Lys 
        275                 280                 285             


Ala Lys Thr Glu Gln Asp Ile Glu Arg Ser Asn Lys Thr Val Gly Glu 
    290                 295                 300                 


Phe Ile Tyr Glu Thr Leu Leu Glu Asn Pro Thr Gln Lys Ile Arg Gly 
305                 310                 315                 320 


Lys Leu Val Lys Thr Ile Glu Arg Lys Phe Tyr Lys Glu Glu Leu Lys 
                325                 330                 335     


Asn Ile Leu Ile Lys Gln Ile Glu Leu Gln Pro Thr Leu Phe Ser Glu 
            340                 345                 350         


Glu Leu Tyr Lys Ala Cys Ile Glu Glu Leu Tyr Pro Arg Asn Glu Ala 
        355                 360                 365             


His Gln Asn Ala Ile Lys Asn Arg Asp Phe Ile Tyr Leu Leu Met Asp 
    370                 375                 380                 


Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Gln Lys Ser Asn Ile 
385                 390                 395                 400 


Ser Gly Cys Gln Leu Glu Gln Arg Val Tyr Tyr Lys Ala Asn Lys Asp 
                405                 410                 415     


Ser Gly Glu Asn Glu Glu Val Lys Gln Ala Val Lys Ala Ile Pro Lys 
            420                 425                 430         


Ser His Pro Leu Phe Gln Glu Phe Arg Asn Trp Gln Trp Leu Gln Asn 
        435                 440                 445             


Leu Lys Ile Tyr Asn Lys Val Asp Thr Glu Lys Gly Glu Leu Thr Asp 
    450                 455                 460                 


Val Thr Lys Gln Leu Leu Pro Ser Glu Asp Ser Trp Val Asn Leu Phe 
465                 470                 475                 480 


Asn Phe Leu Gln Cys Lys Lys Glu Leu Glu Gln Lys Gln Phe Ile Lys 
                485                 490                 495     


Tyr Phe Ile Asp Lys Lys Leu Ile Asn Lys Thr Glu Lys Asp His Tyr 
            500                 505                 510         


Arg Trp Asn Tyr Val Glu Asp Lys Lys Tyr Pro Phe Thr Glu Thr Arg 
        515                 520                 525             


Ala Gln Phe Ile Ser Arg Leu Thr Lys Val Glu Gly Ile Glu Glu Ile 
    530                 535                 540                 


Asn Thr Phe Leu Asp Lys Lys Thr Gln Ile Gly Thr Lys Asp Asn Ser 
545                 550                 555                 560 


Pro Phe Val Ser Arg Ile Glu Gln Leu Trp His Ile Ile Tyr Ser Val 
                565                 570                 575     


Ser Asp Ile Lys Glu Tyr Glu Ser Ala Leu Glu Lys Phe Ala Ile Lys 
            580                 585                 590         


His His Leu Asn Glu Glu Ser Phe Val Ala Asn Phe Ile Lys Phe Pro 
        595                 600                 605             


Pro Phe Lys Ser Asp Tyr Gly Ser Tyr Ser Lys Lys Ala Leu Asn Lys 
    610                 615                 620                 


Leu Leu Pro Leu Met Arg Arg Gly Asn Tyr Trp Thr Glu Asn Asp Ile 
625                 630                 635                 640 


Ser Glu Lys Val Lys Leu Arg Val Ser Glu Ile Met Glu Arg Val Asn 
                645                 650                 655     


Ala Leu Asn Leu Lys Glu Asp Tyr Ser Ser Lys Glu Leu Ala Glu Thr 
            660                 665                 670         


Leu Val Asn Val Ser Asp Asp Asp Val Lys Lys Gln Leu Ile Lys Ser 
        675                 680                 685             


Phe Val Ser Phe Lys Asp Arg Asn Pro Leu Met Gly Leu Asn Thr Phe 
    690                 695                 700                 


Gln Ala Thr Tyr Leu Val Tyr Gly Arg His Ser Glu Val Gly Asp Ile 
705                 710                 715                 720 


Gln Asn Trp Lys Thr Pro Asp Asp Ile Asp Thr Tyr Leu Lys Asn Phe 
                725                 730                 735     


Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val Thr Glu 
            740                 745                 750         


Thr Leu Arg Val Val Arg Asp Ile Trp Lys His His Gly Lys Ser Gln 
        755                 760                 765             


Thr Asn Phe Phe Asn Glu Ile His Val Glu Leu Gly Arg Glu Met Lys 
    770                 775                 780                 


Asn Pro Ala Asp Lys Arg Lys Arg Ile Ser Glu Arg Asn Thr Glu Asn 
785                 790                 795                 800 


Glu Asn Thr Asn Asn Arg Ile Arg Glu Ile Leu Lys Asp Leu Met Asn 
                805                 810                 815     


Asp Ala Ser Ile Gln Gly Asp Val Arg Asp Tyr Ser Pro Ser Gln Gln 
            820                 825                 830         


Gly Ile Leu Lys Ile Tyr Glu Glu Gly Val Tyr Gln Asn Pro Lys Val 
        835                 840                 845             


Asp Tyr Ser Lys Val Ser Glu Asp Glu Ile Thr Lys Ile Arg Arg Ser 
    850                 855                 860                 


Asn Asn Pro Thr Gln Lys Glu Ile Gln Arg Tyr Arg Leu Trp Leu Glu 
865                 870                 875                 880 


Gln Gly Tyr Ile Ser Pro Tyr Thr Cys Lys Pro Ile Pro Leu Ser Lys 
                885                 890                 895     


Leu Phe Thr His His Tyr Gln Ile Glu His Ile Ile Pro Gln Ser Arg 
            900                 905                 910         


Tyr Phe Asp Asn Ser Ser Ser Asn Lys Ile Ile Cys Glu Ser Glu Val 
        915                 920                 925             


Asn Glu Asp Lys Asp Asn Lys Thr Ala Phe Glu Tyr Leu Lys Asp Lys 
    930                 935                 940                 


Gly Glu Asn Val Ile Asn Gly His Lys Leu Leu Ser Leu Ala Ala Tyr 
945                 950                 955                 960 


Glu Ala His Val Asn Lys Tyr Phe Lys Asn Asn Arg Gln Lys Leu Lys 
                965                 970                 975     


Asn Leu Leu Ser Glu Asp Ile Pro Glu Gly Phe Ile Asn Arg Gln Met 
            980                 985                 990         


Asn Asp Ser Arg Tyr Ile Ser Lys  Leu Val Lys Gly Leu  Leu Ser Asn 
        995                 1000                 1005             


Ile Val  Arg Glu Glu Gly Glu  Gln Glu Val Thr Ser  Lys Asn Leu 
    1010                 1015                 1020             


Ile Pro  Val Thr Gly Ser Val  Thr Ser Lys Leu Lys  Asn Asp Trp 
    1025                 1030                 1035             


Gly Leu  Asn Asp Lys Trp Asn  Glu Leu Ile Leu Pro  Arg Phe Lys 
    1040                 1045                 1050             


Arg Leu  Asn Gln Leu Thr Gln  Thr Asn Asp Phe Thr  Thr Ala Asn 
    1055                 1060                 1065             


Thr Asn  Gly Asn Thr Ile Pro  Thr Val Pro Asp Glu  Leu Leu Lys 
    1070                 1075                 1080             


Gly Phe  Ser Lys Lys Arg Ile  Asp His Arg His His  Ala Leu Asp 
    1085                 1090                 1095             


Ala Leu  Val Val Ala Cys Cys  Thr Arg Asn His Ser  Asn Tyr Leu 
    1100                 1105                 1110             


Ser Ala  Leu Asn Thr Glu Asn  Lys Asn Tyr Gly Leu  Arg Asp Lys 
    1115                 1120                 1125             


Leu Leu  Ile Lys Asn Asp Asn  Asp His Tyr Thr Lys  Ser Phe Gln 
    1130                 1135                 1140             


Met Pro  Trp His Gly Phe Thr  Thr Glu Ala Lys Asn  Gln Leu Glu 
    1145                 1150                 1155             


Glu Thr  Val Ile Ser Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys 
    1160                 1165                 1170             


Ala Asn  Asn Lys Phe Trp Ser  Tyr Lys Asp Glu Asn  Gly Lys Leu 
    1175                 1180                 1185             


Asn Leu  Asp Lys Asn Gly Lys  Pro Ile Lys Lys Leu  Arg Lys Gln 
    1190                 1195                 1200             


Thr Lys  Gly Asp Asn Trp Ala  Val Arg Gln Pro Leu  His Glu Glu 
    1205                 1210                 1215             


Thr Ile  Ser Gly Ile Val Asn  Leu Pro Trp Val Lys  Ala Glu Lys 
    1220                 1225                 1230             


Glu Lys  Phe Thr Tyr Ala Thr  Arg Lys Ser Leu Asp  Lys Ser Phe 
    1235                 1240                 1245             


Asp Leu  Lys Lys Ile Glu Lys  Ile Thr Asp Thr Gly  Ile Gln Lys 
    1250                 1255                 1260             


Ile Leu  Arg Asn Tyr Leu Met  Gln Glu Lys Phe Lys  Glu Leu Asp 
    1265                 1270                 1275             


Lys Lys  Asp Lys Glu Val Tyr  Asn Ser Glu Leu Ala  Phe Ser Gln 
    1280                 1285                 1290             


Glu Gly  Leu Glu Asp Leu Asn  Ser Asn Ile Glu Gln  Phe Asn Asp 
    1295                 1300                 1305             


Gln Lys  Pro His Lys Pro Ile  Tyr Lys Val Arg Leu  Phe Glu Lys 
    1310                 1315                 1320             


Gly Ser  Gly Arg Phe Ala Leu  Gly Phe Lys Gly Ile  Asn Ala Lys 
    1325                 1330                 1335             


Lys Tyr  Ala Gln Gly Ser Pro  Asn Leu Tyr Phe Asn  Ile Tyr Arg 
    1340                 1345                 1350             


Asn Glu  Asp Gly Gln Tyr Tyr  Glu Thr Val Pro Leu  Asn Lys Val 
    1355                 1360                 1365             


Ile Ile  His Gln Lys Asn Ala  Ala Asn Met Pro Lys  Lys Asn Arg 
    1370                 1375                 1380             


Thr Pro  Val Pro Ile Ser Asn  Thr Ala Ile Asn Arg  Ser Lys Glu 
    1385                 1390                 1395             


Val Leu  Val Asp Tyr Lys Met  Thr Leu Ser Pro Leu  Asp Leu Val 
    1400                 1405                 1410             


Tyr Ile  Pro Thr Glu Glu Glu  Leu Asn Asn Ile Asn  Ser Leu Asp 
    1415                 1420                 1425             


Phe Asn  Asn Leu Ser Lys Ser  Gln Arg Lys Arg Leu  Tyr Asn Val 
    1430                 1435                 1440             


Asn Asp  Phe Ser Gly Tyr Thr  Ile Tyr Phe Ser Pro  Asn Thr Leu 
    1445                 1450                 1455             


Ala Lys  Ser Ile Cys Pro Lys  Glu Val Asp Met Lys  Phe Asp Glu 
    1460                 1465                 1470             


Lys Lys  Gln Lys Pro Thr Gly  Ser Phe Asp Ser Lys  Thr Ala Ser 
    1475                 1480                 1485             


Leu Asp  Gly Met Ser Ile Lys  Glu Arg Cys Ile Lys  Leu Ile Ile 
    1490                 1495                 1500             


Asp Arg  Leu Gly Asn Ile Ser  Lys Ala 
    1505                 1510         


<210>  1321
<211>  1512
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1321

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Ala Asp Glu Ile Thr Arg Asp Asp Gly Ser Arg Tyr 
            20                  25                  30          


Leu Lys Pro Asn Gly Ile Ser Ala Ala Gly Ser Arg Ile Ile Pro Met 
        35                  40                  45              


Ser Ala Asp Ile Leu Gly Asp Phe Glu Lys Gly Asn Ser Ile Ser Gln 
    50                  55                  60                  


Thr Ala Glu Arg Thr Arg Met Arg Met Ala Arg Arg Leu His Glu Arg 
65                  70                  75                  80  


Ala Leu Leu Arg Arg Glu Arg Leu Leu Arg Val Leu Asn Leu Leu Asp 
                85                  90                  95      


Phe Leu Pro Lys His Phe Ala Asp Lys Ile Asp Arg Tyr Gly Lys Phe 
            100                 105                 110         


Thr Asp Asp Ser Glu Pro Lys Leu Ala Trp Arg Lys Asn Thr Glu Gly 
        115                 120                 125             


Lys Tyr Glu Phe Ile Phe Gln Asp Ala Phe Asn Glu Met Leu Ala Glu 
    130                 135                 140                 


Phe Lys Asp Lys Gln Pro Glu Ile Val Glu Glu Gly Lys Lys Ile Pro 
145                 150                 155                 160 


Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Glu Lys Ala 
                165                 170                 175     


Leu Ser Lys Glu Glu Leu Ser Trp Ile Leu Leu Gln Phe Asn Gln Lys 
            180                 185                 190         


Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu Ile Pro Gln Asp 
        195                 200                 205             


Lys Lys Ile Glu Tyr Leu Ala Gln Lys Val Val Lys Val Glu Ala Thr 
    210                 215                 220                 


Asp Gln Lys Lys Gly Asp Asp Val Trp Tyr Asn Val Tyr Leu Glu Asn 
225                 230                 235                 240 


Gly Met Ile Tyr Arg Arg Thr Ser Lys Ala Pro Leu Asp Trp Glu Gly 
                245                 250                 255     


Lys Val Lys Glu Phe Ile Val Thr Thr Glu Val Glu Asn Asp Gly Glu 
            260                 265                 270         


Thr Pro Lys Lys Asp Lys Glu Gly Asn Ile Lys Arg Ser Phe Arg Ala 
        275                 280                 285             


Pro Lys Glu Asp Asp Trp Thr Leu Leu Lys Lys Lys Thr Glu Ala Asp 
    290                 295                 300                 


Ile Glu Asn Ser His Lys Thr Val Gly Cys Tyr Ile Tyr Asp Ser Leu 
305                 310                 315                 320 


Leu Asn Asn Pro Lys Gln Lys Ile Ile Gly Lys Leu Val Arg Thr Val 
                325                 330                 335     


Glu Arg Lys Phe Tyr Lys Glu Glu Leu Thr Gln Ile Leu Lys Lys Gln 
            340                 345                 350         


Val Glu Leu Ile Pro Glu Leu Arg Asp Asp Asn Leu Tyr Lys Gln Cys 
        355                 360                 365             


Ile Glu Glu Leu Tyr Pro Ile Asn Glu Ala His Arg Asn Thr Ile Ala 
    370                 375                 380                 


Lys Thr Asp Phe Ala Asn Leu Phe Ile Asn Asp Ile Leu Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Gln Ile Asp Asn Cys Pro Tyr Glu 
                405                 410                 415     


Glu Arg Ile Phe Ile Asp Ser Lys Thr Gly Glu Lys Lys Lys Val Pro 
            420                 425                 430         


Val Lys Cys Ile Ala Lys Ser Asn Pro Leu Phe Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Ile Gln Asn Leu Arg Ile Tyr Gln Arg Glu Lys Glu Ile 
    450                 455                 460                 


Asp Gly Lys Leu Leu Thr Asp Ile Asp Ile Thr Ser Glu Cys Leu Lys 
465                 470                 475                 480 


Ser Glu Glu Asp Tyr Val Arg Leu Phe Asp Trp Leu Asn Asp Arg Glu 
                485                 490                 495     


Thr Ile Glu Gln Glu Asp Leu Leu Lys His Leu Phe Asn Thr Lys Lys 
            500                 505                 510         


Ser Lys Asn Lys Glu Asn Pro Tyr Arg Trp Asn Tyr Val Glu Asp Lys 
        515                 520                 525             


Val Tyr Pro Cys Asn Glu Thr Arg Ala Thr Ile Leu Lys Gly Phe Ser 
    530                 535                 540                 


Lys Cys Gly Ile Asn Ala Ser Val Leu Ser Pro Glu Leu Glu Met Ala 
545                 550                 555                 560 


Leu Trp His Ile Leu Tyr Ser Val Glu Asp Lys Lys Glu Ile Glu Thr 
                565                 570                 575     


Ala Leu Thr His Phe Ala Gln Lys Gln Gly Trp Asn Asp Asp Phe Ala 
            580                 585                 590         


Lys Val Phe Ser Lys Ala Lys Pro Phe Lys Lys Asp Tyr Gly Ser Tyr 
        595                 600                 605             


Ser Glu Lys Ala Ile Lys Lys Leu Leu Ser Leu Met Arg Met Gly Lys 
    610                 615                 620                 


Tyr Trp Asn Gln Asp Asn Ile Asp Lys Asn Thr Leu Asp Arg Ile Asp 
625                 630                 635                 640 


Lys Ile Ile Asn Gly Glu Tyr Asp Glu Lys Ile Ser Asn Arg Val Arg 
                645                 650                 655     


Asp Asn Ala Ile Asn Leu Lys Asp Ile Ser Asp Phe Arg Gly Leu Pro 
            660                 665                 670         


Val Trp Leu Ala Cys Tyr Ile Val Tyr Asp Arg His Ser Glu Ala Lys 
        675                 680                 685             


Asp Cys Thr Lys Trp Asn Ser Pro Glu Glu Ile Asp Ser Tyr Leu Lys 
    690                 695                 700                 


Lys Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val 
705                 710                 715                 720 


Thr Glu Thr Leu Arg Thr Val Arg Asp Ile Trp Ile Gln Glu Glu Gln 
                725                 730                 735     


Ile Asp Glu Ile His Leu Glu Leu Gly Arg Asp Leu Lys Asn Pro Ala 
            740                 745                 750         


Asp Lys Arg Lys Lys Met Ser Glu Asn Ile Leu Lys Asn Glu Asn Thr 
        755                 760                 765             


Asn Leu Arg Ile Lys Ala Met Leu Met Glu Phe Met Asn Pro Ser Met 
    770                 775                 780                 


Gly Ile Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln Asp Ile Leu 
785                 790                 795                 800 


Arg Ile Tyr Glu Glu Asn Ala Leu Asp Asn Leu Thr Lys Asp Asp Lys 
                805                 810                 815     


Asp Phe Asp Phe Ile Ser Lys Ile Ser Lys Gln Ala Gln Pro Ser Lys 
            820                 825                 830         


Thr Asp Ile Ile Arg Tyr Lys Cys Trp Leu Glu Gln Lys Tyr Arg Ser 
        835                 840                 845             


Pro Tyr Thr Gly Lys Thr Ile Ser Leu Ser Lys Leu Phe Thr Pro Ala 
    850                 855                 860                 


Tyr Glu Ile Glu His Val Ile Pro Gln Ser Leu Tyr Phe Asp Asp Ser 
865                 870                 875                 880 


Phe Ser Asn Lys Val Ile Cys Glu Ala Glu Val Asn Lys Leu Lys Asp 
                885                 890                 895     


Arg Gln Leu Gly Tyr Lys Phe Ile Ala Glu His His Gly Glu Lys Val 
            900                 905                 910         


Gln Leu Ser Gln Gly Glu Val Val Glu Ile Leu Ser Val Asp Ala Tyr 
        915                 920                 925             


Glu Lys Phe Val Lys Glu Asn Tyr Ala Asn Asn Arg Ile Lys Met Lys 
    930                 935                 940                 


Lys Leu Leu Met Glu Ser Ile Pro Asp Glu Phe Ile Glu Arg Gln Leu 
945                 950                 955                 960 


Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Gly Leu Leu Ser Asn 
                965                 970                 975     


Ile Val Arg Glu Lys Ile Asp Asp Glu Asn Tyr Glu Pro Glu Ala Val 
            980                 985                 990         


Ser Lys Asn Leu Ile Ser Cys Asn  Gly Ala Ile Thr Asp  Arg Leu Lys 
        995                 1000                 1005             


Lys Asp  Trp Gly Met Asn Asp  Val Trp Asn Ser Ile  Ile Leu Pro 
    1010                 1015                 1020             


Arg Phe  Ile Arg Met Asn Gln  Ile Thr Gly Lys Asp  Cys Phe Thr 
    1025                 1030                 1035             


Thr Thr  Asn Ala Glu Gly His  Leu Ile Pro Gln Met  Pro Leu Glu 
    1040                 1045                 1050             


Leu Gln  Lys Gly Phe Asn Lys  Lys Arg Ile Asp His  Arg His His 
    1055                 1060                 1065             


Ala Met  Asp Ala Ile Val Ile  Ala Cys Thr Thr Arg  Asp His Val 
    1070                 1075                 1080             


Asn Leu  Leu Asn Asn Glu Ala  Ala His Ser Lys Phe  Asn Ala Thr 
    1085                 1090                 1095             


Arg Tyr  Gln Leu Gln Arg Lys  Leu Arg Arg Phe Glu  Lys Ala Val 
    1100                 1105                 1110             


Ile Asp  Gly Lys Glu Arg Glu  Val Ala Lys Glu Phe  Leu Lys Pro 
    1115                 1120                 1125             


Trp Asp  Ser Phe Ser Ser Asp  Ser Lys Asn Ile Leu  Glu Asn Ile 
    1130                 1135                 1140             


Ile Val  Ser Phe Lys Gln Asn  Gln Arg Val Ile Asn  Lys Thr Thr 
    1145                 1150                 1155             


Asn Ala  Phe Leu His Phe Asp  Glu Asn Gly Lys Lys  Thr Phe Ile 
    1160                 1165                 1170             


Lys Gln  Gly Lys Gly Asp Ser  Trp Ala Ile Arg Lys  Ser Met His 
    1175                 1180                 1185             


Lys Asp  Thr Val Phe Gly Glu  Ile Asn Leu Arg Lys  Val Arg Ser 
    1190                 1195                 1200             


Val Ser  Leu Asn Glu Ala Ile  Lys Val Pro Glu Arg  Val Leu Asn 
    1205                 1210                 1215             


Lys Lys  Ile Lys Glu Lys Ile  Leu Glu Leu Lys Asn  Asn Lys Ile 
    1220                 1225                 1230             


Asp Ala  Lys Asn Ile Lys Lys  Tyr Ile Glu Glu Asn  His Thr Gly 
    1235                 1240                 1245             


Gly Tyr  Gly Ile Asp Ser Ser  Lys Ile Asp Val Phe  Tyr Phe Thr 
    1250                 1255                 1260             


Lys Glu  Thr Lys Glu Arg Phe  Phe Ala Thr Arg Lys  Thr Leu Asp 
    1265                 1270                 1275             


Thr Ser  Phe Asn Gln Ala Lys  Ile Lys Asp Ser Ile  Ala Asp Ser 
    1280                 1285                 1290             


Gly Ile  Gln Lys Ile Leu Leu  Ala His Leu Lys Ser  Lys Asn Gly 
    1295                 1300                 1305             


Asp Ala  Glu Gln Ala Phe Ser  Pro Asp Gly Ile Asp  Glu Met Asn 
    1310                 1315                 1320             


Lys Asn  Ile Val Glu Leu Asn  Asn Gly Lys Phe His  Gln Pro Ile 
    1325                 1330                 1335             


Leu Lys  Val Arg Val Tyr Glu  Lys Ala Asp Lys Phe  Ala Val Gly 
    1340                 1345                 1350             


Gln Lys  Gly Asn Lys Lys Val  Lys Phe Val Glu Ala  Ala Lys Gly 
    1355                 1360                 1365             


Thr Asn  Leu Phe Phe Ala Ile  Phe Glu Lys Asp Gly  Lys Arg Ser 
    1370                 1375                 1380             


Tyr Leu  Thr Ile Pro Leu Asn  Val Met Ile Asp Cys  Gln Lys Gln 
    1385                 1390                 1395             


Tyr Gly  Asn Gln Trp Lys Asn  Asn Ile Glu Ala Tyr  Leu Lys Glu 
    1400                 1405                 1410             


Lys Glu  Leu Val Glu Lys Asp  Val Lys Leu Gln Phe  Ile Leu Ser 
    1415                 1420                 1425             


Pro Asn  Asp Leu Val Tyr Leu  Pro Thr Glu Gly Ser  Ile Asp Lys 
    1430                 1435                 1440             


Lys Arg  Ile Tyr Lys Val Val  Ser Phe Thr Asn Asn  Arg Leu Tyr 
    1445                 1450                 1455             


Ala Ile  Pro Tyr Ala Ile Ala  Lys Ser Ile Val Asp  Lys Asn Glu 
    1460                 1465                 1470             


Phe Thr  Gln Leu Asn Lys Ile  Glu Phe Thr Asp Asn  Lys Glu Ser 
    1475                 1480                 1485             


Ile Lys  Asp Ala Cys Val Pro  Val Ile Ile Asp Arg  Leu Gly Asn 
    1490                 1495                 1500             


Val Ile  Glu Phe Asn Gly Lys  Arg Leu 
    1505                 1510         


<210>  1322
<211>  1512
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1322

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Ile Asn Ser Glu Glu Asp Gly Pro Ile Val Lys Lys Arg Ile 
            20                  25                  30          


Glu Leu Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Val Leu Gly 
        35                  40                  45              


Asn Phe Asp Ser Gly Val Thr Lys Ser Gln Thr Ala Glu Arg Thr Ser 
    50                  55                  60                  


Phe Arg Ser Ile Arg Arg Leu Arg Glu Arg Gln Leu Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Lys Val Leu Gln Ile Leu Gly Phe Leu Pro Glu His Tyr 
                85                  90                  95      


Val Asn Asp Ile Asp Phe Glu Asn His Pro Gly Lys Ile Lys Arg Asn 
            100                 105                 110         


Thr Glu Pro Lys Leu Pro Trp Arg Lys Asn Ser Met Gly Gln Phe Glu 
        115                 120                 125             


Phe Ile Phe Gln Asp Ser Phe Glu Ala Met Leu Thr Asp Phe Ala Thr 
    130                 135                 140                 


His Gln Pro Val Phe Val Ser Glu Gly Lys Lys Val Pro Tyr Asp Trp 
145                 150                 155                 160 


Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Glu Gly Ile Ser Lys 
                165                 170                 175     


Glu Glu Leu Ala Trp Leu Ile Leu Asn Phe Asn Gln Lys Arg Gly Tyr 
            180                 185                 190         


Tyr Gln Leu Arg Gly Glu Glu Glu Glu Glu Gln Ser Asn Lys Leu Val 
        195                 200                 205             


Glu Phe Tyr Ala Leu Arg Val Val Lys Val Glu Ala Thr Asp Asp Lys 
    210                 215                 220                 


Lys Gly Lys Asp Thr Trp Tyr Asn Val His Leu Glu Asn Gly Trp Ile 
225                 230                 235                 240 


Tyr Arg Arg Thr Ser Asn Ile Pro Leu Asp Trp Glu Gly Lys Val Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Asp Leu Asn Glu Asp Gly Thr Pro Lys Leu 
            260                 265                 270         


Asp Lys Tyr Gly Glu Val Lys Arg Ser Phe Arg Ala Pro Lys Glu Asp 
        275                 280                 285             


Asp Trp Thr Leu Ile Lys Lys Lys Thr Glu Ser Asp Ile Ala Asn Ser 
    290                 295                 300                 


His Lys Thr Val Gly Cys Tyr Ile Tyr Asp Ala Leu Leu Gln Asn Pro 
305                 310                 315                 320 


His Gln Lys Ile Lys Gly Arg Leu Val Arg Thr Val Glu Arg Lys Phe 
                325                 330                 335     


Tyr Lys Glu Glu Leu Arg Leu Ile Leu Glu Lys Gln Leu Ser Phe His 
            340                 345                 350         


Pro Glu Leu Gln Asp Arg Asp Leu Tyr Arg Ala Cys Ile Asp Ala Leu 
        355                 360                 365             


Tyr Val Ser Asn Glu Ala His Lys Asn Asn Ile Ser Gly Arg Asp Phe 
    370                 375                 380                 


Val Tyr Leu Phe Ile Asp Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys 
385                 390                 395                 400 


Thr Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu Asn Gln Tyr 
                405                 410                 415     


Met Asp Arg Glu Thr Gly Glu Val Lys Gln Ala Pro Ile Lys Cys Ile 
            420                 425                 430         


Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Ile 
        435                 440                 445             


Ser Asn Ile Arg Ile Tyr Gln Arg Glu Arg Val Leu Asp Asp Gly Lys 
    450                 455                 460                 


Leu Glu Thr Asp Val Asp Val Thr Pro Asp Phe Leu Lys Thr Glu Asp 
465                 470                 475                 480 


Asp Tyr Thr Thr Leu Phe Glu Trp Phe Asn Asp Arg Lys Glu Val Asp 
                485                 490                 495     


Gln Lys Ala Phe Leu Lys Asn Pro Gln Phe Gly Leu Lys Lys Thr Phe 
            500                 505                 510         


Thr Glu Tyr Arg Trp Asn Tyr Val Glu Asp Lys Ser Tyr Pro Cys Asn 
        515                 520                 525             


Glu Thr Arg Asn Ala Ile Leu Asn Gly Leu Gln Lys Ala Gly Val Ser 
    530                 535                 540                 


Ala Asp Phe Leu Ser Arg Glu Val Glu Glu Ala Leu Trp His Ile Leu 
545                 550                 555                 560 


Tyr Ser Val Glu Asp Lys Ile Glu Ile Glu Ser Ala Leu Arg Lys Phe 
                565                 570                 575     


Ala Ser Lys His Asp Leu Asp Asp Arg Phe Ala Asp Val Phe Lys Lys 
            580                 585                 590         


Leu Pro Pro Phe Lys Lys Glu Tyr Gly Ala Tyr Ser Ala Lys Ala Ile 
        595                 600                 605             


Lys Lys Leu Leu Pro Leu Met Arg Ile Gly Lys Tyr Trp Glu Leu Ser 
    610                 615                 620                 


Asn Ile Asp Asp Lys Thr Lys Glu Arg Ile Asp Lys Ile Ile Thr Gly 
625                 630                 635                 640 


Glu Trp Asp Glu Asn Ile His Asp Arg Val Arg Glu Lys Ala Ile Lys 
                645                 650                 655     


Leu Ser Asp Ile Thr Asp Phe Lys Gly Leu Pro Leu Trp Leu Ser Cys 
            660                 665                 670         


Tyr Val Val Tyr Asn Arg His Ser Glu Ala Arg Ala Ile Ala Lys Trp 
        675                 680                 685             


Glu Ser Pro Glu Asp Ile Asp Ser Tyr Leu Arg Leu Phe Arg Gln His 
    690                 695                 700                 


Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu Arg 
705                 710                 715                 720 


Thr Val Arg Asp Ile Trp Lys Arg Val Gly Gln Ile Asp Glu Ile His 
                725                 730                 735     


Val Glu Leu Gly Arg Glu Ile Lys Asn Pro Ala Asp Lys Arg Ala Lys 
            740                 745                 750         


Met Thr Ala Gln Ile Val Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys 
        755                 760                 765             


Ala Leu Leu Ala Glu Phe Ile Asn Ser Glu Tyr Glu Ile Glu Asn Val 
    770                 775                 780                 


Arg Pro Tyr Ser Ser Asn Gln Gln Glu Lys Leu Lys Ile Tyr Glu Asp 
785                 790                 795                 800 


Thr Val Leu Asn Gly Val Lys Glu Ile Pro Glu Asp Ile Asn Thr Ile 
                805                 810                 815     


Leu Lys Lys Phe Arg Glu Ser Asp Leu Lys Lys Arg Pro Thr Lys Ser 
            820                 825                 830         


Asp Phe Leu His Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Cys Ser Pro 
        835                 840                 845             


Tyr Thr Gly Arg Ile Ile Pro Leu Gly Lys Leu Phe Thr Ser Ala Tyr 
    850                 855                 860                 


Glu Ile Glu His Ile Ile Pro Gln Ala His Tyr Phe Asp Asp Ser Phe 
865                 870                 875                 880 


Ser Asn Lys Val Ile Cys Glu Ala Glu Val Asn Gly Leu Lys Asp Lys 
                885                 890                 895     


Lys Leu Gly Tyr Glu Phe Ile Lys His Asn Pro Gly Lys Met Val Glu 
            900                 905                 910         


Ile Gly Phe Gly Lys Pro Pro Val Arg Ile Phe Ser Val Glu Glu Tyr 
        915                 920                 925             


Glu Arg Phe Val Lys Glu Asn Tyr Ser Gly Ile Lys Asn Lys Met Asn 
    930                 935                 940                 


Lys Leu Leu Met Asp Asp Ile Pro Glu Thr Phe Ile Glu Arg Gln Leu 
945                 950                 955                 960 


Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser Leu Leu Ser Asn 
                965                 970                 975     


Ile Val Arg Glu Lys Asp Ser Asn Gly Glu Tyr Glu Pro Glu Ala Ile 
            980                 985                 990         


Ser Lys Asn Val Ile Val Cys Thr  Gly Ser Val Thr Asp  Arg Leu Lys 
        995                 1000                 1005             


Arg Asp  Trp Gly Val Asn Asp  Val Trp Asn Ser Ile  Ile Tyr Pro 
    1010                 1015                 1020             


Arg Phe  Glu Arg Leu Asn Glu  Leu Thr Gly Ser Gln  Gln Phe Gly 
    1025                 1030                 1035             


Cys Trp  Glu Asn Lys Glu Gly  Lys Arg Val Phe Gln  Thr Ala Val 
    1040                 1045                 1050             


Pro Leu  Glu Met Gln Lys Gly  Phe Ser Lys Lys Arg  Ile Asp His 
    1055                 1060                 1065             


Arg His  His Ala Met Asp Ala  Ile Val Ile Ala Cys  Ala Thr Arg 
    1070                 1075                 1080             


Asn His  Ile Asn Tyr Leu Ser  Asn Glu Ser Ala Ser  Lys Lys Ala 
    1085                 1090                 1095             


Lys Ile  Ser Arg Thr Asp Leu  Gln Arg Leu Leu Cys  Thr Lys Cys 
    1100                 1105                 1110             


Arg Thr  Asp Asp Asn Gly Asn  Tyr Lys Trp Ile Val  Lys Lys Pro 
    1115                 1120                 1125             


Trp Pro  Ala Phe Thr Gln Asp  Ile Arg Lys Ala Leu  Glu Asn Ile 
    1130                 1135                 1140             


Thr Val  Ser Phe Lys Gln Asn  Leu Arg Val Ile Asn  Lys Thr Thr 
    1145                 1150                 1155             


Asn Tyr  Tyr Val His Phe Asp  Glu Ser Gly Lys Lys  Val Leu Glu 
    1160                 1165                 1170             


Lys Gln  Ile Asn Gly Asp Ser  Trp Ala Ile Arg Lys  Pro Met His 
    1175                 1180                 1185             


Lys Asp  Thr Val Phe Gly Lys  Val Asn Leu Gln Lys  Val Lys Glu 
    1190                 1195                 1200             


Val Arg  Leu Ser Leu Ala Leu  Asp Thr Pro Lys Met  Ile Val Asp 
    1205                 1210                 1215             


Lys Lys  Leu Lys Glu Lys Val  Leu Gln Leu Tyr Ser  Tyr Lys Tyr 
    1220                 1225                 1230             


Asp Lys  Lys Arg Ile Asp Lys  Tyr Phe Lys Glu Asn  Ala Ser Leu 
    1235                 1240                 1245             


Trp Lys  Asp Leu Asn Leu Ser  Lys Val Ala Val Tyr  Tyr Phe Thr 
    1250                 1255                 1260             


Asn Asp  Ser Thr Glu Pro Leu  Val Ala Val Arg Lys  Pro Leu Asp 
    1265                 1270                 1275             


Thr Ser  Phe Asn Ala Lys Lys  Ile Lys Glu Ser Val  Thr Asp Thr 
    1280                 1285                 1290             


Gly Ile  Gln Lys Ile Leu Leu  Asn His Leu Ser Asn  Asn Gln Asp 
    1295                 1300                 1305             


Asp Pro  Asn Leu Ala Phe Ser  Pro Asp Gly Ile Asp  Lys Met Asn 
    1310                 1315                 1320             


Lys Asn  Leu Thr Ala Leu Asn  Asp Gly Lys Tyr His  Gln Pro Ile 
    1325                 1330                 1335             


Tyr Lys  Val Arg Val Cys Glu  Pro Gln Gly Asn Lys  Phe Ser Val 
    1340                 1345                 1350             


Gly Thr  Ser Gly Asn Lys Thr  Ser Lys Tyr Val Glu  Ala Ala Lys 
    1355                 1360                 1365             


Gly Thr  Asn Leu Phe Phe Ala  Val Tyr Gln Thr Glu  Asp Gly Lys 
    1370                 1375                 1380             


Arg Ser  Tyr Glu Thr Ile Pro  Leu Asn Val Val Ile  Glu Arg Glu 
    1385                 1390                 1395             


Lys Leu  Gly Trp Thr Pro Val  Pro Glu Asn Asn Asn  Asn Gly Asp 
    1400                 1405                 1410             


Lys Leu  Leu Phe Trp Leu Ser  Pro Asn Asp Leu Val  Tyr Val Pro 
    1415                 1420                 1425             


Thr Ala  Asp Glu Leu Asn Asn  Gly Met Arg Leu Asp  Asn Met Asp 
    1430                 1435                 1440             


Lys Gly  Arg Ile Tyr Lys Met  Ile Ser Cys Ser Gly  Pro Gln Cys 
    1445                 1450                 1455             


Phe Phe  Ile Pro Gln Asn Val  Ser Asn Ala Ile Ile  Pro Val Ile 
    1460                 1465                 1470             


Glu Leu  Gly Ala Asn Asn Lys  Ala Glu Lys Ser Trp  Asn Asn Glu 
    1475                 1480                 1485             


Met Ile  Lys Val Val Cys Val  Pro Ile Arg Val Asp  Arg Leu Gly 
    1490                 1495                 1500             


Cys Ile  Thr Gln Ile Asn Asn  Gln Lys 
    1505                 1510         


<210>  1323
<211>  1516
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1323

Met Lys Asn Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ser Val Ile Gln Glu Lys His Asn Glu Asn Asn Glu Cys Gln Lys Leu 
            20                  25                  30          


Ile Leu Gly Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Val Leu 
        35                  40                  45              


Gly Asp Phe Ala Lys Gly Asn Thr Ile Ser Gln Thr Lys Asp Arg Thr 
    50                  55                  60                  


Gln Tyr Arg Gly Val Arg Arg Leu Arg Glu Arg Cys Leu Leu Arg Arg 
65                  70                  75                  80  


Glu Arg Leu Phe Arg Val Leu Asp Ile Met Gly Phe Leu Pro Gln His 
                85                  90                  95      


Phe Ser Asn Ala Leu Thr Arg Tyr Gly Lys Phe Lys Asp Ala Asp Glu 
            100                 105                 110         


Glu Pro Lys Ile Ala Trp Arg Lys Asp Asp Glu Gly Lys Met His Phe 
        115                 120                 125             


Leu Phe Ala Asp Ser Phe Asn Glu Met Ile Glu Glu Phe Arg Ser Val 
    130                 135                 140                 


Gln Pro Glu Leu Leu Lys Arg Gly Met Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Leu Tyr Tyr Leu Arg Lys Lys Ala Met Arg Glu Ala Val Thr Pro Phe 
                165                 170                 175     


Glu Leu Ala Trp Leu Ile Leu Ser Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Gly Glu Ala Asp Asn Ser Lys Ser Glu 
        195                 200                 205             


Glu Tyr Leu Ala Gln Arg Val Val Asp Val Ile Asp Thr Gly Glu Lys 
    210                 215                 220                 


Lys Gly Lys Ser Thr Trp Phe Asp Ile Val Leu Glu Asn Gly Met Val 
225                 230                 235                 240 


Tyr His Arg Pro Ala Glu Ile Lys Pro Asp Trp Ile Gly Lys Thr Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Gln Phe Asp Lys Asp Gly Asn Pro Lys Lys 
            260                 265                 270         


Asp Lys Glu Gly Asn Val Lys Met Ser Phe Arg Met Pro Asn Glu Asn 
        275                 280                 285             


Asp Trp Asn Leu Gln Lys Lys Lys Ala Glu Lys Asp Ile Asp Glu Ser 
    290                 295                 300                 


Gly Met Thr Val Gly Glu Tyr Ile Tyr Asn Ala Leu Leu His Asn Pro 
305                 310                 315                 320 


Lys Gln Lys Ile Lys Gly Lys Leu Val Arg Val Val Glu Arg Lys Tyr 
                325                 330                 335     


Tyr Lys Asn Glu Leu Gln Lys Ile Leu Glu Ser Gln Lys Arg Phe Ile 
            340                 345                 350         


Pro Gln Leu Ser Asp Ser Ala Leu Tyr Glu Lys Cys Ile Lys Glu Leu 
        355                 360                 365             


Tyr Gln Ser Asn Glu Ala Tyr Arg Asn Ser Ile Ser Thr Arg Asn Asp 
    370                 375                 380                 


Phe Thr Tyr Leu Phe Val Asp Asn Ile Ile Phe Tyr Gln Arg Pro Leu 
385                 390                 395                 400 


Lys Ser Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Ser Leu Ala 
                405                 410                 415     


Tyr Thr Lys Asp Gly Glu Glu His Lys Ala Pro Val Lys Cys Ile Ala 
            420                 425                 430         


Lys Ser His Pro Leu Tyr Gln Glu Phe Arg Leu Trp Gln Phe Ile Ser 
        435                 440                 445             


Asn Leu Arg Ile Tyr Gln Arg Gln Lys Gln Val Gly Asp Lys Val Lys 
    450                 455                 460                 


Leu Asp Val Asp Val Thr Asp Glu Phe Leu Pro Thr Asp Asp Arg Arg 
465                 470                 475                 480 


Glu Ser Leu Phe Asp Tyr Leu Asn Asp Lys Thr Glu Ile Lys Gln Asp 
                485                 490                 495     


Val Leu Leu Lys Glu Phe Phe Ser Met Lys Lys Pro Lys Gly Lys Asp 
            500                 505                 510         


Ser Val Tyr Pro Tyr Arg Trp Asn Tyr Val Glu Asp Lys Ser Tyr Pro 
        515                 520                 525             


Cys Asn Val Thr Arg Gly Lys Ile Leu Ala Lys Leu Asn Arg Ala Gly 
    530                 535                 540                 


Ile Ser Ala Asp Phe Leu Ser Ser Asn Gly Asn Glu Glu Ser Leu Trp 
545                 550                 555                 560 


His Ile Leu Tyr Ser Val Glu Asp Lys Gly Glu Leu Arg Lys Ala Leu 
                565                 570                 575     


Ala Arg Phe Ala Ala Lys Asn Gly Leu Asp Asp Ala Phe Thr Glu Ala 
            580                 585                 590         


Leu Ala Asp Ile Glu Pro Tyr Lys Lys Glu Tyr Gly Ala Tyr Ser Ala 
        595                 600                 605             


Lys Ala Leu Lys Lys Leu Leu Pro Leu Met Arg Leu Gly Lys Tyr Trp 
    610                 615                 620                 


Asn Tyr Asp Asn Ile Ala Pro Ala Thr Lys Ala Arg Ile Ser Lys Ile 
625                 630                 635                 640 


Ile Asp Gly Glu Tyr Asp Glu Asn Ile Arg Asn Arg Val Arg Glu Lys 
                645                 650                 655     


Ala Met Ser Leu Asn Ser Ala Ser Gln Phe Gln Gly Leu Pro Leu Trp 
            660                 665                 670         


Leu Ala Cys Tyr Ile Val Tyr Asp Arg His Ser Glu Arg Ala Asp Ser 
        675                 680                 685             


Gly Arg Trp Ser Ser Pro Glu Asp Ile Asp Lys Tyr Leu Ser Asp Phe 
    690                 695                 700                 


Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val Thr Glu 
705                 710                 715                 720 


Thr Leu Arg Thr Val Arg Asp Ile Trp Lys Arg Tyr Gly Glu Ile Ser 
                725                 730                 735     


Glu Ile His Val Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Asp Lys 
            740                 745                 750         


Arg Ser Lys Met Asn Arg Arg Met Leu Glu Asn Glu Asn Ala Asn Met 
        755                 760                 765             


Arg Ala Lys Leu Leu Leu Thr Glu Phe Met Asn Pro Glu Phe Gly Ile 
    770                 775                 780                 


Glu Asn Val Arg Ser Tyr Ser Pro Ser Gln Gln Glu Leu Leu Arg Ile 
785                 790                 795                 800 


Tyr Glu Asp Gly Ala Ile Ser Asn Ala Gly Ser Glu Ile Asp Asp Glu 
                805                 810                 815     


Ile Ser Asp Ile Met Ser Arg Leu Ser Gln Val Asp Val Lys Lys Gln 
            820                 825                 830         


Pro Thr Ala Ser Glu Val Lys Arg Tyr Ile Leu Trp Leu Glu Gln Asn 
        835                 840                 845             


Tyr Arg Ser Pro Tyr Thr Gly Gln Met Ile Pro Leu Ala Lys Leu Phe 
    850                 855                 860                 


Thr Pro Ala Tyr Glu Ile Glu His Val Ile Pro Gln Ser Arg Tyr Phe 
865                 870                 875                 880 


Asp Asp Ser Phe Ser Asn Lys Val Ile Cys Glu Ala Glu Val Asn Lys 
                885                 890                 895     


Leu Lys Asp Arg Met Leu Gly Tyr Glu Phe Ile Lys Gln His Lys Gly 
            900                 905                 910         


Glu Lys Val Ser Leu Ser Met Gly Gly Thr Val Glu Ile Leu Ser Pro 
        915                 920                 925             


Glu Glu Tyr Val Arg His Val Glu Ser Gln Tyr Lys Asn Asn Lys Ala 
    930                 935                 940                 


Lys Met Arg Lys Leu Leu Met Asp Asp Ile Pro Asp Glu Phe Ile Asn 
945                 950                 955                 960 


Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Leu Ile Ile Ser Leu 
                965                 970                 975     


Leu Ser Asn Ile Val Arg Glu Glu Gly Glu Glu Glu Ala Thr Ser Lys 
            980                 985                 990         


Asn Val Ile Ser Cys Asn Gly Ser  Ile Thr Asp Arg Leu  Lys Lys Asp 
        995                 1000                 1005             


Trp Gly  Ile Asn Asp Val Trp  Asn His Ile Ile Leu  Pro Arg Phe 
    1010                 1015                 1020             


Arg Arg  Leu Asn Glu Leu Thr  Gly Thr Asp Lys Phe  Thr Thr Ser 
    1025                 1030                 1035             


Asn Lys  Glu Gly His Glu Val  Pro Glu Met Pro Leu  Glu Leu Gln 
    1040                 1045                 1050             


Lys Gly  Phe Asn Lys Lys Arg  Ile Asp His Arg His  His Ala Met 
    1055                 1060                 1065             


Asp Ala  Ile Val Ile Ala Cys  Ile Thr Arg Asp His  Val Asn Leu 
    1070                 1075                 1080             


Leu Asn  Asn Glu Ala Ala Met  Ser Lys Asn Asn Ala  Asn Arg Ile 
    1085                 1090                 1095             


Gln Leu  Ser Lys Lys Leu Arg  Arg Tyr Glu Ser Val  Glu Ile Glu 
    1100                 1105                 1110             


Lys Asp  Gly Ile Arg Lys Gln  Ile Ser Met Ala Lys  Glu Phe Lys 
    1115                 1120                 1125             


Lys Pro  Trp Glu Gly Phe Thr  Ala Asp Val Glu Arg  Thr Leu Arg 
    1130                 1135                 1140             


Asp Ile  Val Val Ser Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys 
    1145                 1150                 1155             


Thr Thr  Asn Tyr Tyr Gln His  Phe Lys Asp Gly Lys  Lys Ile Tyr 
    1160                 1165                 1170             


Ser Pro  Gln Ser Lys Gly Asp  Ser Trp Ala Ile Arg  Lys Ser Met 
    1175                 1180                 1185             


His Lys  Glu Thr Val Phe Gly  Glu Ile Asn Ile Arg  Arg Ile Lys 
    1190                 1195                 1200             


Glu Val  Ser Leu Lys Glu Ala  Met Lys Arg Ile Lys  Asp Ile Ala 
    1205                 1210                 1215             


Asp Lys  Glu Leu Arg Ser Lys  Ile Val Glu Leu Met  Ala Leu Gln 
    1220                 1225                 1230             


Tyr Asn  Glu Lys Lys Ile Lys  Gln Tyr Phe Ser Asp  Asn Lys Asp 
    1235                 1240                 1245             


Glu Trp  Ser Asp Val Asn Pro  Lys Lys Ile Lys Ile  His Tyr Phe 
    1250                 1255                 1260             


Thr Lys  Glu Thr Asn Glu Arg  Phe Phe Ala Thr Arg  Lys Pro Leu 
    1265                 1270                 1275             


Asp Thr  Ser Phe Asp Lys Lys  Lys Ile Lys Thr Gln  Val Ala Asp 
    1280                 1285                 1290             


Thr Ala  Ile Gln Lys Ile Leu  Leu Asn His Leu Ser  Ala His Gly 
    1295                 1300                 1305             


Asp Lys  Ala Glu Thr Ala Phe  Ser Pro Asp Gly Ile  Asp Glu Met 
    1310                 1315                 1320             


Asn Arg  Asn Ile Ile Glu Leu  Asn Asp Gly Lys Phe  His Gln Pro 
    1325                 1330                 1335             


Ile Leu  Lys Val Arg Ile Tyr  Glu Lys Ala Asp Lys  Tyr Ala Val 
    1340                 1345                 1350             


Gly Gln  Gln Gly Asn Lys Lys  Lys Lys Tyr Val Glu  Ala Ala Lys 
    1355                 1360                 1365             


Gly Thr  Asn Leu Phe Phe Ala  Ile Tyr Ala Glu Glu  Lys Ile Asp 
    1370                 1375                 1380             


Glu Lys  Thr Gly Asp Thr Val  Ile Thr Arg Thr Tyr  Asp Ser Ile 
    1385                 1390                 1395             


Pro Leu  Asn Val Val Ile Asp  Arg Glu Lys Arg Gly  Leu Ser Pro 
    1400                 1405                 1410             


Val Pro  Glu Asn Glu Asn Gly  Ala Lys Pro Lys Tyr  Val Leu Ser 
    1415                 1420                 1425             


Pro Asn  Asp Leu Val Tyr Val  Pro Asn Glu Glu Glu  Arg Glu Thr 
    1430                 1435                 1440             


Gly Arg  Ile Cys Glu Pro Leu  Asp Lys Glu Arg Ile  Tyr Lys Met 
    1445                 1450                 1455             


Val Ser  Ser Asn Lys Lys Gln  Ala Phe Phe Ile Lys  Ser Glu Val 
    1460                 1465                 1470             


Ala Ser  Thr Ile Val Asp Lys  Phe Glu Phe Ser Ala  Leu Asn Lys 
    1475                 1480                 1485             


Gln Glu  Arg Ala Leu Ser Gly  Glu Met Ile Lys Glu  Ile Cys Ile 
    1490                 1495                 1500             


Pro Ile  Ser Ile Asn Arg Ile  Gly Glu Ile Thr Leu  Arg 
    1505                 1510                 1515     


<210>  1324
<211>  1517
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1324

Met Lys Lys Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Asn Ala Tyr Ile Asn Lys Glu His Leu Tyr Gly Ile Glu 
            20                  25                  30          


Ala Cys Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile Leu Gly Asn 
        35                  40                  45              


Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ala Asp Arg Thr Ser Tyr 
    50                  55                  60                  


Arg Gly Ile Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg 
65                  70                  75                  80  


Leu His Arg Ile Leu Asp Leu Leu Gly Phe Leu Pro Lys His Tyr Ser 
                85                  90                  95      


Asp Ser Leu Asn Arg Tyr Gly Lys Phe Leu Asn Asp Ile Glu Cys Lys 
            100                 105                 110         


Leu Pro Trp Val Lys Asp Glu Thr Gly Ser Tyr Lys Phe Ile Phe Gln 
        115                 120                 125             


Glu Ser Phe Lys Glu Met Leu Ala Asn Phe Thr Glu His His Pro Ile 
    130                 135                 140                 


Leu Ile Ala Asn Asn Lys Lys Val Pro Tyr Asp Trp Thr Ile Tyr Tyr 
145                 150                 155                 160 


Leu Arg Lys Lys Ala Leu Thr Gln Lys Ile Ser Lys Glu Glu Leu Ala 
                165                 170                 175     


Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg 
            180                 185                 190         


Gly Glu Glu Glu Glu Thr Pro Asn Lys Leu Val Glu Tyr Tyr Ser Leu 
        195                 200                 205             


Lys Val Glu Lys Val Glu Asp Ser Gly Glu Arg Lys Gly Lys Asp Thr 
    210                 215                 220                 


Trp Tyr Asn Val His Leu Glu Asn Gly Met Ile Tyr Arg Arg Thr Ser 
225                 230                 235                 240 


Asn Ile Pro Leu Asp Trp Glu Gly Lys Thr Lys Glu Phe Ile Val Thr 
                245                 250                 255     


Thr Asp Leu Glu Ala Asp Gly Ser Pro Lys Lys Asp Lys Glu Gly Asn 
            260                 265                 270         


Ile Lys Arg Ser Phe Arg Ala Pro Lys Asp Asp Asp Trp Thr Leu Ile 
        275                 280                 285             


Lys Lys Lys Thr Glu Ala Asp Ile Asp Lys Ile Lys Met Thr Val Gly 
    290                 295                 300                 


Ala Tyr Ile Tyr Asp Thr Leu Leu Gln Lys Pro Asp Gln Lys Ile Arg 
305                 310                 315                 320 


Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr Lys Asn Glu Leu 
                325                 330                 335     


Tyr Gln Ile Leu Lys Thr Gln Ser Glu Phe His Glu Glu Leu Arg Asp 
            340                 345                 350         


Lys Gln Leu Tyr Ile Ala Cys Leu Asn Glu Leu Tyr Pro Asn Asn Glu 
        355                 360                 365             


Pro Arg Arg Asn Ser Ile Ser Thr Arg Asp Phe Cys His Leu Phe Ile 
    370                 375                 380                 


Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser Leu 
385                 390                 395                 400 


Ile Asp Asn Cys Pro Tyr Glu Glu Asn Arg Tyr Ile Asp Lys Glu Ser 
                405                 410                 415     


Gly Glu Ile Lys His Ala Ser Ile Lys Cys Ile Ala Lys Ser His Pro 
            420                 425                 430         


Leu Tyr Gln Glu Phe Arg Leu Trp Gln Phe Ile Val Asn Leu Arg Ile 
        435                 440                 445             


Tyr Arg Lys Glu Thr Asp Val Asp Val Thr Gln Glu Leu Leu Pro Thr 
    450                 455                 460                 


Glu Ala Asp Tyr Val Thr Leu Phe Glu Trp Leu Asn Glu Lys Lys Glu 
465                 470                 475                 480 


Ile Asp Gln Lys Ala Phe Phe Lys Tyr Pro Pro Phe Gly Phe Lys Lys 
                485                 490                 495     


Thr Thr Ser Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys Pro Tyr Pro 
            500                 505                 510         


Cys Asn Glu Thr His Ala Gln Ile Ile Ala Arg Leu Gly Lys Ala His 
        515                 520                 525             


Ile Pro Lys Ala Phe Leu Ser Lys Glu Lys Glu Glu Thr Leu Trp His 
    530                 535                 540                 


Ile Leu Tyr Ser Ile Glu Asp Lys Gln Glu Ile Glu Lys Ala Leu His 
545                 550                 555                 560 


Ser Phe Ala Asn Lys Asn Asn Leu Ser Glu Glu Phe Ile Glu Gln Phe 
                565                 570                 575     


Lys Asn Phe Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr Ser Ala Lys 
            580                 585                 590         


Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Met Gly Lys Tyr Trp Ser 
        595                 600                 605             


Ile Glu Asn Ile Asp Asn Gly Thr Arg Ile Arg Ile Asn Lys Ile Ile 
    610                 615                 620                 


Asp Gly Glu Tyr Asp Glu Asn Ile Arg Glu Arg Val Arg Gln Lys Ala 
625                 630                 635                 640 


Ile Asn Leu Thr Asp Ile Thr His Phe Arg Ala Leu Pro Leu Trp Leu 
                645                 650                 655     


Ala Cys Tyr Leu Val Tyr Asp Arg His Ser Glu Val Lys Asp Ile Val 
            660                 665                 670         


Lys Trp Lys Thr Pro Lys Asp Ile Asp Leu Tyr Leu Lys Ser Phe Lys 
        675                 680                 685             


Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr 
    690                 695                 700                 


Leu Arg Thr Val Arg Asp Ile Trp Gln Gln Val Gly His Ile Asp Glu 
705                 710                 715                 720 


Ile His Ile Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Asp Lys Arg 
                725                 730                 735     


Ala Arg Met Ser Gln Gln Met Ile Lys Asn Glu Asn Thr Asn Leu Arg 
            740                 745                 750         


Ile Lys Ala Leu Leu Thr Glu Phe Leu Asn Pro Glu Phe Gly Ile Glu 
        755                 760                 765             


Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln Asp Leu Leu Arg Ile Tyr 
    770                 775                 780                 


Glu Glu Gly Val Leu Asn Ser Ile Leu Glu Leu Pro Glu Asp Ile Gly 
785                 790                 795                 800 


Ile Ile Leu Gly Lys Phe Asn Gln Thr Asp Thr Leu Lys Arg Pro Thr 
                805                 810                 815     


Arg Ser Glu Ile Leu Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg 
            820                 825                 830         


Ser Pro Tyr Thr Gly Glu Met Ile Pro Leu Ser Lys Leu Phe Thr Pro 
        835                 840                 845             


Ala Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Phe Asp Asp 
    850                 855                 860                 


Ser Leu Ser Asn Lys Val Ile Cys Glu Ser Glu Ile Asn Lys Leu Lys 
865                 870                 875                 880 


Asp Arg Ser Leu Gly Tyr Glu Phe Ile Lys Asn His His Gly Glu Lys 
                885                 890                 895     


Val Glu Leu Ala Phe Asp Lys Pro Val Glu Val Leu Ser Val Glu Ala 
            900                 905                 910         


Tyr Glu Lys Leu Val His Glu Ser Tyr Ser His Asn Arg Ser Lys Met 
        915                 920                 925             


Lys Lys Leu Leu Met Glu Asp Ile Pro Asp Gln Phe Ile Glu Arg Gln 
    930                 935                 940                 


Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser Leu Leu Ser 
945                 950                 955                 960 


Asn Ile Val Arg Glu Glu Asn Glu Gln Glu Ala Ile Ser Lys Asn Val 
                965                 970                 975     


Ile Pro Cys Thr Gly Gly Ile Thr Asp Arg Leu Lys Lys Asp Trp Gly 
            980                 985                 990         


Ile Asn Asp Val Trp Asn Lys Ile  Val Leu Pro Arg Phe  Ile Arg Leu 
        995                 1000                 1005             


Asn Glu  Leu Thr Glu Ser Thr  Arg Phe Thr Ser Ile  Asn Thr Asn 
    1010                 1015                 1020             


Asn Thr  Met Ile Pro Ser Met  Pro Leu Glu Leu Gln  Lys Gly Phe 
    1025                 1030                 1035             


Asn Lys  Lys Arg Ile Asp His  Arg His His Ala Met  Asp Ala Ile 
    1040                 1045                 1050             


Ile Ile  Ala Cys Ala Asn Arg  Asn Ile Val Asn Tyr  Leu Asn Asn 
    1055                 1060                 1065             


Val Ser  Ala Ser Lys Asn Thr  Lys Ile Thr Arg Arg  Asp Leu Gln 
    1070                 1075                 1080             


Thr Leu  Leu Cys His Lys Asp  Lys Thr Asp Asn Asn  Gly Asn Tyr 
    1085                 1090                 1095             


Lys Trp  Val Ile Asp Lys Pro  Trp Glu Thr Phe Thr  Gln Asp Thr 
    1100                 1105                 1110             


Leu Thr  Ala Leu Gln Lys Ile  Thr Val Ser Phe Lys  Gln Asn Leu 
    1115                 1120                 1125             


Arg Val  Ile Asn Lys Thr Thr  Asn His Tyr Gln His  Tyr Glu Asn 
    1130                 1135                 1140             


Gly Lys  Lys Ile Val Ser Asn  Gln Ser Lys Gly Asp  Ser Trp Ala 
    1145                 1150                 1155             


Ile Arg  Lys Ser Met His Lys  Glu Thr Val His Gly  Glu Val Asn 
    1160                 1165                 1170             


Leu Arg  Met Ile Lys Thr Val  Ser Phe Asn Glu Ala  Leu Lys Lys 
    1175                 1180                 1185             


Pro Gln  Ala Ile Val Glu Met  Asp Leu Lys Lys Lys  Ile Leu Ala 
    1190                 1195                 1200             


Met Leu  Glu Leu Gly Tyr Asp  Thr Lys Arg Ile Lys  Asn Tyr Phe 
    1205                 1210                 1215             


Glu Glu  Asn Lys Asp Thr Trp  Gln Asp Ile Asn Pro  Ser Lys Ile 
    1220                 1225                 1230             


Lys Val  Tyr Tyr Phe Thr Lys  Glu Thr Lys Asp Arg  Tyr Phe Ala 
    1235                 1240                 1245             


Val Arg  Lys Pro Ile Asp Thr  Ser Phe Asp Lys Lys  Lys Ile Lys 
    1250                 1255                 1260             


Glu Ser  Ile Thr Asp Thr Gly  Ile Gln Gln Ile Met  Leu Arg His 
    1265                 1270                 1275             


Leu Glu  Thr Lys Asp Asn Asp  Pro Thr Leu Ala Phe  Ser Pro Asp 
    1280                 1285                 1290             


Gly Ile  Asp Glu Met Asn Arg  Asn Ile Leu Ile Leu  Asn Lys Gly 
    1295                 1300                 1305             


Lys Lys  His Gln Pro Ile Tyr  Lys Val Arg Val Tyr  Glu Lys Ala 
    1310                 1315                 1320             


Glu Lys  Phe Thr Val Gly Gln  Lys Gly Asn Lys Arg  Thr Lys Phe 
    1325                 1330                 1335             


Val Glu  Ala Ala Lys Gly Thr  Asn Leu Phe Phe Ala  Ile Tyr Glu 
    1340                 1345                 1350             


Thr Glu  Glu Ile Asp Lys Asp  Thr Lys Lys Val Ile  Arg Lys Arg 
    1355                 1360                 1365             


Ser Tyr  Ser Thr Ile Pro Leu  Asn Val Val Ile Glu  Arg Gln Lys 
    1370                 1375                 1380             


Gln Gly  Leu Ser Ser Ala Pro  Glu Asp Glu Asn Gly  Asn Leu Pro 
    1385                 1390                 1395             


Lys Tyr  Ile Leu Ser Pro Asn  Asp Leu Val Tyr Val  Pro Thr Gln 
    1400                 1405                 1410             


Glu Glu  Ile Asn Lys Gly Glu  Val Val Met Pro Ile  Asp Arg Asp 
    1415                 1420                 1425             


Arg Ile  Tyr Lys Met Val Asp  Ser Ser Gly Ile Thr  Ala Asn Phe 
    1430                 1435                 1440             


Ile Pro  Ala Ser Thr Ala Asn  Leu Ile Phe Ala Leu  Pro Lys Ala 
    1445                 1450                 1455             


Thr Ala  Glu Ile Tyr Cys Asn  Gly Glu Asn Cys Ile  Gln Asn Glu 
    1460                 1465                 1470             


Tyr Gly  Ile Gly Ser Pro Gln  Ser Lys Asn Gln Lys  Ala Ile Thr 
    1475                 1480                 1485             


Gly Glu  Met Val Lys Glu Ile  Cys Pro Phe Ile Lys  Val Asp Arg 
    1490                 1495                 1500             


Leu Gly  Asn Ile Ile Gln Val  Gly Ser Cys Ile Leu  Thr Asn 
    1505                 1510                 1515         


<210>  1325
<211>  1520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1325

Met Lys Lys Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Asn Ala Tyr Ile Asp Glu Tyr Asn Lys Glu His Leu Tyr 
            20                  25                  30          


Gly Ile Glu Ala Cys Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile 
        35                  40                  45              


Leu Gly Asn Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ala Asp Arg 
    50                  55                  60                  


Thr Ser Tyr Arg Gly Ile Arg Arg Leu Arg Glu Arg His Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Ile Leu Asp Leu Leu Gly Phe Leu Pro Lys 
                85                  90                  95      


His Tyr Ser Asp Ser Leu Asn Arg Tyr Gly Lys Phe Leu Asn Asp Ile 
            100                 105                 110         


Glu Cys Lys Leu Pro Trp Val Lys Asp Glu Thr Gly Ser Tyr Lys Phe 
        115                 120                 125             


Ile Phe Gln Glu Ser Phe Lys Glu Met Leu Ala Asn Phe Thr Glu His 
    130                 135                 140                 


His Pro Ile Leu Ile Ala Asn Asn Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Lys Ile Ser Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Glu Thr Pro Asn Lys Leu Val Glu Tyr 
        195                 200                 205             


Tyr Ser Leu Lys Val Glu Lys Val Glu Asp Ser Gly Glu Arg Lys Gly 
    210                 215                 220                 


Lys Asp Thr Trp Tyr Asn Val His Leu Glu Asn Gly Met Ile Tyr Arg 
225                 230                 235                 240 


Arg Thr Ser Asn Ile Pro Leu Asp Trp Glu Gly Lys Thr Lys Glu Phe 
                245                 250                 255     


Ile Val Thr Thr Asp Leu Glu Ala Asp Gly Ser Pro Lys Lys Asp Lys 
            260                 265                 270         


Glu Gly Asn Ile Lys Arg Ser Phe Arg Ala Pro Lys Asp Asp Asp Trp 
        275                 280                 285             


Thr Leu Ile Lys Lys Lys Thr Glu Ala Asp Ile Asp Lys Ile Lys Met 
    290                 295                 300                 


Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Gln Lys Pro Asp Gln 
305                 310                 315                 320 


Lys Ile Arg Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr Lys 
                325                 330                 335     


Asn Glu Leu Tyr Gln Ile Leu Lys Thr Gln Ser Glu Phe His Glu Glu 
            340                 345                 350         


Leu Arg Asp Lys Gln Leu Tyr Ile Ala Cys Leu Asn Glu Leu Tyr Pro 
        355                 360                 365             


Asn Asn Glu Pro Arg Arg Asn Ser Ile Ser Thr Arg Asp Phe Cys His 
    370                 375                 380                 


Leu Phe Ile Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
385                 390                 395                 400 


Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu Asn Arg Tyr Ile Asp 
                405                 410                 415     


Lys Glu Ser Gly Glu Ile Lys His Ala Ser Ile Lys Cys Ile Ala Lys 
            420                 425                 430         


Ser His Pro Leu Tyr Gln Glu Phe Arg Leu Trp Gln Phe Ile Val Asn 
        435                 440                 445             


Leu Arg Ile Tyr Arg Lys Glu Thr Asp Val Asp Val Thr Gln Glu Leu 
    450                 455                 460                 


Leu Pro Thr Glu Ala Asp Tyr Val Thr Leu Phe Glu Trp Leu Asn Glu 
465                 470                 475                 480 


Lys Lys Glu Ile Asp Gln Lys Ala Phe Phe Lys Tyr Pro Pro Phe Gly 
                485                 490                 495     


Phe Lys Lys Thr Thr Ser Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys 
            500                 505                 510         


Pro Tyr Pro Cys Asn Glu Thr His Ala Gln Ile Ile Ala Arg Leu Gly 
        515                 520                 525             


Lys Ala His Ile Pro Lys Ala Phe Leu Ser Lys Glu Lys Glu Glu Thr 
    530                 535                 540                 


Leu Trp His Ile Leu Tyr Ser Ile Glu Asp Lys Gln Glu Ile Glu Lys 
545                 550                 555                 560 


Ala Leu His Ser Phe Ala Asn Lys Asn Asn Leu Ser Glu Glu Phe Ile 
                565                 570                 575     


Glu Gln Phe Lys Asn Phe Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr 
            580                 585                 590         


Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Met Gly Lys 
        595                 600                 605             


Tyr Trp Ser Ile Glu Asn Ile Asp Asn Gly Thr Arg Ile Arg Ile Asn 
    610                 615                 620                 


Lys Ile Ile Asp Gly Glu Tyr Asp Glu Asn Ile Arg Glu Arg Val Arg 
625                 630                 635                 640 


Gln Lys Ala Ile Asn Leu Thr Asp Ile Thr His Phe Arg Ala Leu Pro 
                645                 650                 655     


Leu Trp Leu Ala Cys Tyr Leu Val Tyr Asp Arg His Ser Glu Val Lys 
            660                 665                 670         


Asp Ile Val Lys Trp Lys Ile Pro Lys Asp Ile Asp Leu Tyr Leu Lys 
        675                 680                 685             


Ser Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile 
    690                 695                 700                 


Thr Glu Thr Leu Arg Thr Val Arg Asp Ile Trp Gln Gln Val Gly His 
705                 710                 715                 720 


Ile Asp Glu Ile His Ile Glu Leu Gly Arg Glu Met Lys Asn Pro Ala 
                725                 730                 735     


Asp Lys Arg Ala Arg Met Ser Gln Gln Met Ile Lys Asn Glu Asn Thr 
            740                 745                 750         


Asn Leu Arg Ile Lys Ala Leu Leu Thr Glu Phe Leu Asn Pro Glu Phe 
        755                 760                 765             


Gly Ile Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln Asp Leu Leu 
    770                 775                 780                 


Arg Ile Tyr Glu Glu Gly Val Leu Asn Ser Ile Leu Glu Leu Pro Glu 
785                 790                 795                 800 


Asp Ile Gly Ile Ile Leu Gly Lys Phe Asn Gln Thr Asp Thr Leu Lys 
                805                 810                 815     


Arg Pro Thr Arg Ser Glu Ile Leu Arg Tyr Lys Leu Trp Leu Glu Gln 
            820                 825                 830         


Lys Tyr Arg Ser Pro Tyr Thr Gly Glu Met Ile Pro Leu Ser Lys Leu 
        835                 840                 845             


Phe Thr Pro Ala Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr 
    850                 855                 860                 


Phe Asp Asp Ser Leu Ser Asn Lys Val Ile Cys Glu Ser Glu Ile Asn 
865                 870                 875                 880 


Lys Leu Lys Asp Arg Ser Leu Gly Tyr Glu Phe Ile Lys Asn His His 
                885                 890                 895     


Gly Glu Lys Val Glu Leu Ala Phe Asp Lys Pro Val Glu Val Leu Ser 
            900                 905                 910         


Val Glu Ala Tyr Glu Lys Leu Val His Glu Ser Tyr Ser His Asn Arg 
        915                 920                 925             


Ser Lys Met Lys Lys Leu Leu Met Glu Asp Ile Pro Asp Gln Phe Ile 
    930                 935                 940                 


Glu Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser 
945                 950                 955                 960 


Leu Leu Ser Asn Ile Val Arg Glu Glu Asn Glu Gln Glu Ala Ile Ser 
                965                 970                 975     


Lys Asn Val Ile Pro Cys Thr Gly Gly Ile Thr Asp Arg Leu Lys Lys 
            980                 985                 990         


Asp Trp Gly Ile Asn Asp Val Trp  Asn Lys Ile Val Leu  Pro Arg Phe 
        995                 1000                 1005             


Ile Arg  Leu Asn Glu Leu Thr  Glu Ser Thr Arg Phe  Thr Ser Ile 
    1010                 1015                 1020             


Asn Thr  Asn Asn Thr Met Ile  Pro Ser Met Pro Leu  Glu Leu Gln 
    1025                 1030                 1035             


Lys Gly  Phe Asn Lys Lys Arg  Ile Asp His Arg His  His Ala Met 
    1040                 1045                 1050             


Asp Ala  Ile Ile Ile Ala Cys  Ala Asn Arg Asn Ile  Val Asn Tyr 
    1055                 1060                 1065             


Leu Asn  Asn Val Ser Ala Ser  Lys Asn Thr Lys Ile  Thr Arg Arg 
    1070                 1075                 1080             


Asp Leu  Gln Thr Leu Leu Cys  His Lys Asp Lys Thr  Asp Asn Asn 
    1085                 1090                 1095             


Gly Asn  Tyr Lys Trp Val Ile  Asp Lys Pro Trp Glu  Thr Phe Thr 
    1100                 1105                 1110             


Gln Asp  Thr Leu Thr Ala Leu  Gln Lys Ile Thr Val  Ser Phe Lys 
    1115                 1120                 1125             


Gln Asn  Leu Arg Val Ile Asn  Lys Thr Thr Asn His  Tyr Gln His 
    1130                 1135                 1140             


Tyr Glu  Asn Gly Lys Lys Ile  Val Ser Asn Gln Ser  Lys Gly Asp 
    1145                 1150                 1155             


Ser Trp  Ala Ile Arg Lys Ser  Met His Lys Glu Thr  Val His Gly 
    1160                 1165                 1170             


Glu Val  Asn Leu Arg Met Ile  Lys Thr Val Ser Phe  Asn Glu Ala 
    1175                 1180                 1185             


Leu Lys  Lys Pro His Ala Ile  Val Glu Met Asp Leu  Lys Lys Lys 
    1190                 1195                 1200             


Ile Leu  Ala Met Leu Glu Leu  Gly Tyr Asp Thr Lys  Arg Ile Lys 
    1205                 1210                 1215             


Asn Tyr  Phe Glu Glu Asn Lys  Asp Thr Trp Gln Asp  Ile Asn Pro 
    1220                 1225                 1230             


Ser Lys  Ile Lys Val Tyr Tyr  Phe Thr Lys Glu Thr  Lys Asp Arg 
    1235                 1240                 1245             


Tyr Phe  Ala Val Arg Lys Pro  Ile Asp Thr Ser Phe  Asp Lys Lys 
    1250                 1255                 1260             


Lys Ile  Lys Glu Ser Ile Thr  Asp Thr Gly Ile Gln  Gln Ile Met 
    1265                 1270                 1275             


Leu Arg  His Leu Glu Thr Lys  Asp Asn Asp Pro Thr  Leu Ala Phe 
    1280                 1285                 1290             


Ser Pro  Asp Gly Ile Asp Glu  Met Asn Arg Asn Ile  Leu Ile Leu 
    1295                 1300                 1305             


Asn Lys  Gly Lys Lys His Gln  Pro Ile Tyr Lys Val  Arg Val Tyr 
    1310                 1315                 1320             


Glu Lys  Ala Glu Lys Phe Thr  Val Gly Gln Lys Gly  Asn Lys Arg 
    1325                 1330                 1335             


Thr Lys  Phe Val Glu Ala Ala  Lys Gly Thr Asn Leu  Phe Phe Ala 
    1340                 1345                 1350             


Ile Tyr  Glu Thr Glu Glu Ile  Asp Lys Asp Thr Lys  Lys Val Ile 
    1355                 1360                 1365             


Arg Lys  Arg Ser Tyr Ser Thr  Ile Pro Leu Asn Val  Val Ile Glu 
    1370                 1375                 1380             


Arg Gln  Lys Gln Gly Leu Ser  Ser Ala Pro Glu Asp  Glu Asn Gly 
    1385                 1390                 1395             


Asn Leu  Pro Lys Tyr Ile Leu  Ser Pro Asn Asp Leu  Val Tyr Val 
    1400                 1405                 1410             


Pro Thr  Gln Glu Glu Ile Asn  Lys Gly Glu Val Val  Met Pro Ile 
    1415                 1420                 1425             


Asp Arg  Asp Arg Ile Tyr Lys  Met Val Asp Ser Ser  Gly Ile Thr 
    1430                 1435                 1440             


Ala Asn  Phe Ile Pro Ala Ser  Thr Ala Asn Leu Ile  Phe Ala Leu 
    1445                 1450                 1455             


Pro Lys  Ala Thr Ala Glu Ile  Tyr Cys Asn Gly Glu  Asn Cys Ile 
    1460                 1465                 1470             


Gln Asn  Glu Tyr Gly Ile Gly  Ser Pro Gln Ser Lys  Asn Gln Lys 
    1475                 1480                 1485             


Ala Ile  Thr Gly Glu Met Val  Lys Glu Ile Cys Pro  Phe Ile Lys 
    1490                 1495                 1500             


Val Asp  Arg Leu Gly Asn Ile  Ile Gln Val Gly Ser  Cys Ile Leu 
    1505                 1510                 1515             


Thr Asn  
    1520 


<210>  1326
<211>  1520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1326

Met Lys Lys Ile Val Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Asn Ala Tyr Ile Asp Glu Tyr Asn Lys Glu His Leu Tyr 
            20                  25                  30          


Gly Ile Glu Ala Cys Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile 
        35                  40                  45              


Leu Gly Asn Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ala Asp Arg 
    50                  55                  60                  


Thr Ser Tyr Arg Gly Ile Arg Arg Leu Arg Glu Arg His Leu Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu His Arg Ile Leu Asp Leu Leu Gly Phe Leu Pro Lys 
                85                  90                  95      


His Tyr Ser Asp Ser Leu Asn Arg Tyr Gly Lys Phe Leu Asn Asp Ile 
            100                 105                 110         


Glu Cys Lys Leu Pro Trp Val Lys Asp Glu Thr Gly Ser Tyr Lys Phe 
        115                 120                 125             


Ile Phe Gln Glu Ser Phe Lys Glu Met Leu Ala Asn Phe Thr Glu His 
    130                 135                 140                 


His Pro Ile Leu Ile Ala Asn Asn Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Lys Ile Ser Lys Glu 
                165                 170                 175     


Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Glu Glu Thr Pro Asn Lys Leu Val Glu Tyr 
        195                 200                 205             


Tyr Ser Leu Lys Val Glu Lys Val Glu Asp Ser Gly Glu Lys Lys Gly 
    210                 215                 220                 


Lys Asp Thr Trp Tyr Asn Val His Leu Glu Asn Gly Met Ile Tyr Arg 
225                 230                 235                 240 


Arg Thr Ser Asn Ile Pro Leu Asp Trp Glu Gly Lys Thr Lys Glu Phe 
                245                 250                 255     


Ile Val Thr Thr Asp Leu Glu Ala Asp Gly Ser Pro Lys Lys Asp Lys 
            260                 265                 270         


Glu Gly Asn Ile Lys Arg Ser Phe Arg Ala Pro Lys Asp Asp Asp Trp 
        275                 280                 285             


Thr Leu Ile Lys Lys Lys Thr Glu Ala Asp Ile Asp Lys Ile Lys Met 
    290                 295                 300                 


Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Gln Lys Pro Asp Gln 
305                 310                 315                 320 


Lys Ile Arg Gly Lys Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr Lys 
                325                 330                 335     


Asn Glu Leu Tyr Gln Ile Leu Lys Thr Gln Ser Glu Phe His Glu Glu 
            340                 345                 350         


Leu Arg Asp Lys Gln Leu Tyr Ile Ala Cys Leu Asn Glu Leu Tyr Pro 
        355                 360                 365             


Asn Asn Glu Pro Arg Arg Asn Ser Ile Ser Thr Arg Asp Phe Cys His 
    370                 375                 380                 


Leu Phe Ile Glu Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
385                 390                 395                 400 


Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu Asn Arg Tyr Ile Asp 
                405                 410                 415     


Lys Glu Ser Gly Glu Ile Lys His Ala Ser Ile Lys Cys Ile Ala Lys 
            420                 425                 430         


Ser His Pro Leu Tyr Gln Glu Phe Arg Leu Trp Gln Phe Ile Val Asn 
        435                 440                 445             


Leu Arg Ile Tyr Arg Lys Glu Thr Asp Val Asp Val Thr Gln Glu Leu 
    450                 455                 460                 


Leu Pro Thr Glu Ala Asp Tyr Val Thr Leu Phe Glu Trp Leu Asn Glu 
465                 470                 475                 480 


Lys Lys Glu Ile Asp Gln Lys Ala Phe Phe Lys Tyr Pro Pro Phe Gly 
                485                 490                 495     


Phe Lys Lys Thr Thr Ser Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys 
            500                 505                 510         


Pro Tyr Pro Cys Asn Glu Thr His Ala Gln Ile Ile Thr Arg Leu Gly 
        515                 520                 525             


Lys Ala His Ile Pro Lys Ala Phe Leu Ser Lys Glu Lys Glu Glu Thr 
    530                 535                 540                 


Leu Trp His Ile Leu Tyr Ser Ile Glu Asp Lys Gln Glu Ile Glu Lys 
545                 550                 555                 560 


Ala Leu His Ser Phe Ala Asn Lys Asn Asn Leu Ser Glu Glu Phe Ile 
                565                 570                 575     


Glu Gln Phe Lys Asn Phe Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr 
            580                 585                 590         


Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Met Gly Lys 
        595                 600                 605             


Tyr Trp Ser Ile Lys Asn Ile Asp Asn Gly Thr Arg Ile Arg Ile Asn 
    610                 615                 620                 


Lys Ile Ile Asp Gly Glu Tyr Asp Glu Asn Ile Arg Glu Arg Val Arg 
625                 630                 635                 640 


Gln Lys Ala Ile Asn Leu Thr Asp Ile Thr His Phe Arg Ala Leu Pro 
                645                 650                 655     


Leu Trp Leu Ala Cys Tyr Leu Val Tyr Asp Arg His Ser Glu Val Lys 
            660                 665                 670         


Asp Ile Val Lys Trp Lys Thr Pro Lys Asp Ile Asp Leu Tyr Leu Lys 
        675                 680                 685             


Ser Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile 
    690                 695                 700                 


Thr Glu Thr Leu Arg Thr Val Arg Asp Ile Trp Gln Gln Val Gly His 
705                 710                 715                 720 


Ile Asp Glu Ile His Ile Glu Leu Gly Arg Glu Met Lys Ser Pro Ala 
                725                 730                 735     


Asp Lys Arg Ala Arg Met Ser Gln Gln Met Ile Lys Asn Glu Asn Thr 
            740                 745                 750         


Asn Leu Arg Ile Lys Ala Leu Leu Thr Glu Phe Leu Asn Pro Glu Phe 
        755                 760                 765             


Gly Ile Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln Asp Leu Leu 
    770                 775                 780                 


Arg Ile Tyr Glu Glu Gly Val Leu Asn Ser Ile Leu Glu Leu Pro Glu 
785                 790                 795                 800 


Asp Ile Gly Ile Ile Leu Gly Lys Phe Asn Gln Thr Asp Thr Leu Lys 
                805                 810                 815     


Arg Pro Thr Arg Ser Glu Ile Leu Arg Tyr Lys Leu Trp Leu Glu Gln 
            820                 825                 830         


Lys Tyr Arg Ser Pro Tyr Thr Gly Glu Met Ile Pro Leu Ser Lys Leu 
        835                 840                 845             


Phe Thr Pro Ala Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr 
    850                 855                 860                 


Phe Asp Asp Ser Leu Ser Asn Lys Val Ile Cys Glu Ser Glu Ile Asn 
865                 870                 875                 880 


Lys Leu Lys Asp Arg Ser Leu Gly Tyr Glu Phe Ile Lys Asn His His 
                885                 890                 895     


Gly Glu Lys Val Glu Leu Ala Phe Asp Lys Pro Val Glu Val Leu Ser 
            900                 905                 910         


Val Glu Ala Tyr Glu Lys Leu Val His Glu Ser Tyr Ser His Asn Arg 
        915                 920                 925             


Ser Lys Met Lys Lys Leu Leu Met Glu Asp Ile Pro Asp Gln Phe Ile 
    930                 935                 940                 


Glu Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser 
945                 950                 955                 960 


Leu Leu Ser Asn Ile Val Arg Glu Glu Asn Glu Gln Glu Ala Ile Ser 
                965                 970                 975     


Lys Asn Val Ile Pro Cys Thr Gly Gly Ile Thr Asp Arg Leu Lys Lys 
            980                 985                 990         


Asp Trp Gly Ile Asn Asp Val Trp  Asn Lys Ile Val Leu  Pro Arg Phe 
        995                 1000                 1005             


Ile Arg  Leu Asn Glu Leu Thr  Glu Ser Thr Arg Phe  Thr Ser Ile 
    1010                 1015                 1020             


Asn Thr  Asn Asn Thr Met Ile  Pro Ser Met Pro Leu  Glu Leu Gln 
    1025                 1030                 1035             


Lys Gly  Phe Asn Lys Lys Arg  Ile Asp His Arg His  His Ala Met 
    1040                 1045                 1050             


Asp Ala  Ile Ile Ile Ala Cys  Ala Asn Arg Asn Ile  Val Asn Tyr 
    1055                 1060                 1065             


Leu Asn  Asn Val Ser Ala Ser  Lys Asn Thr Lys Ile  Thr Arg Arg 
    1070                 1075                 1080             


Asp Leu  Gln Thr Leu Leu Cys  His Lys Asp Lys Thr  Asp Asn Asn 
    1085                 1090                 1095             


Gly Asn  Tyr Lys Trp Val Ile  Asp Lys Pro Trp Glu  Thr Phe Thr 
    1100                 1105                 1110             


Gln Asp  Thr Leu Thr Ala Leu  Gln Lys Ile Thr Val  Ser Phe Lys 
    1115                 1120                 1125             


Gln Asn  Leu Arg Val Ile Asn  Lys Thr Thr Asn His  Tyr Gln His 
    1130                 1135                 1140             


Tyr Glu  Asn Gly Lys Lys Ile  Val Ser Asn Gln Ser  Lys Gly Asp 
    1145                 1150                 1155             


Ser Trp  Ala Ile Arg Lys Ser  Met His Lys Glu Thr  Val His Gly 
    1160                 1165                 1170             


Glu Val  Asn Leu Arg Met Ile  Lys Thr Val Ser Leu  Asn Glu Ala 
    1175                 1180                 1185             


Leu Lys  Lys Pro Gln Ala Ile  Val Glu Met Asp Leu  Lys Lys Lys 
    1190                 1195                 1200             


Ile Leu  Ala Met Leu Glu Leu  Gly Tyr Asp Thr Lys  Arg Ile Lys 
    1205                 1210                 1215             


Asn Tyr  Phe Glu Glu Asn Lys  Asp Thr Trp Gln Asp  Ile Asn Pro 
    1220                 1225                 1230             


Ser Lys  Ile Lys Val Tyr Tyr  Phe Thr Lys Glu Thr  Lys Asp Arg 
    1235                 1240                 1245             


Tyr Phe  Ala Val Arg Lys Pro  Ile Asp Thr Ser Phe  Asp Lys Lys 
    1250                 1255                 1260             


Lys Ile  Lys Glu Ser Ile Thr  Asp Thr Gly Ile Gln  Gln Ile Met 
    1265                 1270                 1275             


Leu Arg  His Leu Glu Thr Lys  Asp Asn Asp Pro Thr  Leu Ala Phe 
    1280                 1285                 1290             


Ser Pro  Asp Gly Ile Asp Glu  Met Asn Arg Asn Ile  Leu Ile Leu 
    1295                 1300                 1305             


Asn Lys  Gly Lys Lys His Gln  Pro Ile Tyr Lys Val  Arg Val Tyr 
    1310                 1315                 1320             


Glu Lys  Ala Glu Lys Phe Thr  Val Gly Gln Lys Gly  Asn Lys Arg 
    1325                 1330                 1335             


Thr Lys  Phe Val Glu Ala Ala  Lys Gly Thr Asn Leu  Phe Phe Ala 
    1340                 1345                 1350             


Ile Tyr  Glu Thr Glu Glu Ile  Asp Lys Asp Thr Lys  Lys Val Ile 
    1355                 1360                 1365             


Arg Lys  Arg Ser Tyr Ser Thr  Ile Pro Leu Asn Val  Val Ile Glu 
    1370                 1375                 1380             


Arg Gln  Lys Gln Gly Leu Ser  Ser Ala Pro Glu Asp  Glu Asn Gly 
    1385                 1390                 1395             


Asn Leu  Pro Lys Tyr Ile Leu  Ser Pro Asn Asp Leu  Val Tyr Val 
    1400                 1405                 1410             


Pro Thr  Gln Glu Glu Ile Asn  Lys Gly Glu Val Val  Met Pro Ile 
    1415                 1420                 1425             


Asp Arg  Asp Arg Ile Tyr Lys  Met Val Asp Ser Ser  Gly Ile Thr 
    1430                 1435                 1440             


Ala Asn  Phe Ile Pro Ala Ser  Thr Ala Asn Leu Ile  Phe Ala Leu 
    1445                 1450                 1455             


Pro Lys  Ala Thr Ala Glu Ile  Tyr Cys Asn Gly Glu  Asn Cys Ile 
    1460                 1465                 1470             


Gln Asn  Glu Tyr Gly Ile Gly  Ser Pro Gln Ser Lys  Asn Gln Lys 
    1475                 1480                 1485             


Ala Ile  Thr Gly Glu Met Val  Lys Glu Ile Cys Pro  Phe Ile Lys 
    1490                 1495                 1500             


Val Asp  Arg Leu Gly Asn Ile  Ile Gln Val Gly Ser  Cys Ile Leu 
    1505                 1510                 1515             


Thr Asn  
    1520 


<210>  1327
<211>  1524
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1327

Met Asn Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Ile Val Gln Lys Asp Glu Gln Gly Phe Tyr Glu Lys Ile Ile Asn 
            20                  25                  30          


Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Glu Thr Met Asn Asn Phe 
        35                  40                  45              


Asn Lys Gly Ile Thr Lys Ser Pro Ala Ser Glu Arg Thr Arg Phe Arg 
    50                  55                  60                  


Gly Ala Arg Arg Leu Ile Glu Arg Ser Leu Leu Arg Arg Glu Arg Ile 
65                  70                  75                  80  


His Arg Leu Leu Asn Thr Met Asn Tyr Leu Pro Pro His Tyr Ala Glu 
                85                  90                  95      


Lys Ile Asp Phe Val Asn Arg Leu Gly Lys Phe Ile Gly Glu Glu Glu 
            100                 105                 110         


Pro Lys Tyr Ala Tyr Lys Val Asp Glu His Gly Lys Phe Gln Phe Ile 
        115                 120                 125             


Phe Lys Glu Ser Phe Asn Glu Met Leu Lys Asp Phe Gln Asp Lys Gln 
    130                 135                 140                 


Pro Glu Leu Val Leu Asn Asn Lys Lys Val Pro Tyr Asp Trp Thr Ile 
145                 150                 155                 160 


Tyr Tyr Leu Arg Lys Lys Ala Leu Asn Arg Ala Ile Ser Lys Glu Glu 
                165                 170                 175     


Leu Gly Trp Ile Ile Leu Gln Phe Asn Asn Lys Arg Gly Tyr Tyr Gln 
            180                 185                 190         


Leu Arg Gly Glu Glu Asp Glu Thr Leu Thr Glu Gly Gln Lys Lys Glu 
        195                 200                 205             


Tyr Phe Ala Leu Lys Val Ile Arg Val Glu Ala Asp Ser Ser Ser Ala 
    210                 215                 220                 


Gly Lys Lys Gly Glu Val Trp Tyr Asn Val Gln Leu Glu Asn Gly Trp 
225                 230                 235                 240 


Ile Tyr Arg Cys Lys Ser Glu Ile Pro Leu Asp Trp Glu Gly Lys Ile 
                245                 250                 255     


Lys Glu Phe Ile Val Thr Thr Glu Val Asp Glu Asn Asp Asn Val Leu 
            260                 265                 270         


Lys Asp Lys Glu Gly Lys Glu Lys Arg Lys Leu Lys Ile Pro Glu Ala 
        275                 280                 285             


Gly Asp Trp Thr Leu Leu Lys Lys Lys Thr Glu Ser Asp Leu Asp Lys 
    290                 295                 300                 


Ser Gly Lys Thr Val Gly Ala Tyr Ile Tyr Asp Thr Leu Leu Ser Asn 
305                 310                 315                 320 


Pro Asp Gln Lys Ile Lys Gly Gly Leu Ile Arg Thr Ile Glu Arg Glu 
                325                 330                 335     


Leu Tyr Arg Ser Glu Leu Glu Gln Ile Leu Asp Lys Gln Lys Glu Phe 
            340                 345                 350         


His Pro Glu Leu Lys Asp Gly Thr Leu Tyr Asn Lys Cys Val Glu Glu 
        355                 360                 365             


Leu Tyr Arg Ser Asn Glu Ser Arg Arg Ile Ser Leu Ser Asn Tyr Asp 
    370                 375                 380                 


Phe Thr Arg Phe Ile Thr Glu Asp Val Ile Phe Tyr Gln Arg Pro Leu 
385                 390                 395                 400 


Lys Ser Lys Lys Ser Leu Ile Asp Asn Cys Ser Phe Glu Arg Arg Asn 
                405                 410                 415     


Tyr Leu Asn Pro Ile Thr Lys Glu Leu Ala Tyr Ala Pro Ile Lys Cys 
            420                 425                 430         


Ile Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe 
        435                 440                 445             


Ile Lys Asn Leu Arg Ile Ile Glu Arg Glu Lys Ile Val Gly Asp Lys 
    450                 455                 460                 


Leu Met Phe Asp Gln Asp Val Thr Thr Glu Phe Leu Pro Thr Gln Asn 
465                 470                 475                 480 


Asp Tyr Val Ile Leu Phe Asp Trp Leu Asn Glu His Lys Glu Ile Asp 
                485                 490                 495     


Gln Lys Ala Leu Leu Lys Tyr Pro Ala Phe Asn Leu Lys Lys Asn Ile 
            500                 505                 510         


Asp Lys Tyr Arg Trp Asn Tyr Val Glu Asn Lys Ser Tyr Pro Cys Asn 
        515                 520                 525             


Glu Thr Arg Ala Leu Leu Lys Ala Lys Leu Asn Lys Ala Gly Asn Ile 
    530                 535                 540                 


Pro Ser Asp Phe Leu Asp Asn Asp Thr Leu Glu Ser Leu Trp His Ile 
545                 550                 555                 560 


Leu Tyr Ser Val Glu Asp Lys Phe Glu Ile Glu Lys Ala Leu Lys Ser 
                565                 570                 575     


Phe Ala Thr Lys Lys Glu Leu Arg Glu Glu Gln Thr Val Ala Phe Val 
            580                 585                 590         


Lys Gln Phe Ile Asn Ile Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr 
        595                 600                 605             


Ser Ala Lys Ala Ile Lys Lys Leu Leu Gln Leu Met Arg Met Gly Ser 
    610                 615                 620                 


Arg Trp Ser Glu Asn Glu Ile Ser Asp Ser Thr Lys Asp Arg Ile Gln 
625                 630                 635                 640 


Lys Leu Ile Asp Gly Glu Trp Asp Glu Ser Ile Lys Thr Arg Thr Arg 
                645                 650                 655     


Asp Lys Ala Ile Lys Leu Asn Glu Ile Asn Asp Phe Gln Gly Leu Pro 
            660                 665                 670         


Leu Trp Leu Ala Ser Tyr Ile Val Tyr Asp Arg His Ser Glu Ser Gly 
        675                 680                 685             


Asp Val Met Lys Trp Glu Thr Pro Glu Asp Ile Asp Tyr Tyr Leu Lys 
    690                 695                 700                 


His Val Phe Lys Gln His Gly Leu Arg Asn Pro Ile Val Glu Val Val 
705                 710                 715                 720 


Val Val Glu Thr Leu Lys Val Val Lys Asp Leu Trp Lys Thr Tyr Gly 
                725                 730                 735     


Tyr Phe Asn Glu Ile His Ile Glu Leu Gly Arg Glu Met Lys Asn Pro 
            740                 745                 750         


Ala Asp Lys Arg Arg Ser Met Thr Asn Lys Asn Ile Glu Asn Glu Asn 
        755                 760                 765             


Thr Asn Leu Arg Ile Lys Ala Leu Leu Ala Glu Leu Ala Asn His Lys 
    770                 775                 780                 


Asp Val Glu Gly Val Arg Pro Tyr Ser Pro Ser Gln Gln Glu Ile Leu 
785                 790                 795                 800 


Lys Ile Tyr Glu Glu Gly Val Leu Asp Met Leu Thr Lys Glu Asp Pro 
                805                 810                 815     


Asp Tyr Glu Ser Ile Ser Lys Ile Ser Lys Met Ala Leu Pro Ser Ser 
            820                 825                 830         


Ser Glu Leu Ile Arg Tyr Lys Cys Trp Leu Glu Gln Lys Tyr Arg Ser 
        835                 840                 845             


Pro Tyr Thr Gly Gln Val Ile Pro Leu Ala Arg Leu Phe Thr Ser Glu 
    850                 855                 860                 


Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser 
865                 870                 875                 880 


Phe Ser Asn Lys Val Ile Cys Glu Ser Ala Val Asn Lys Asp Lys Asp 
                885                 890                 895     


Asn Leu Leu Ala Tyr Glu Tyr Ile Lys Gln Asn Glu Gly Lys Ile Ile 
            900                 905                 910         


Glu Ile Gly Leu Gly Lys Lys Val Lys Leu Phe Thr Ala Asp Ser Tyr 
        915                 920                 925             


Thr Glu Phe Val Gln Ser Asn Tyr Val Gly Ser Val Ala Lys Lys Lys 
    930                 935                 940                 


Lys Leu Leu Met Asp Thr Ile Pro Asp Ser Phe Ile Lys Arg Gln Leu 
945                 950                 955                 960 


Asn Asp Ser Arg Tyr Ile Ser Arg Glu Ile Met Lys Leu Leu Ser Ser 
                965                 970                 975     


Val Val Arg Glu Lys Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Ala 
            980                 985                 990         


Ile Ser Lys Asn Val Ile Val Cys  Thr Gly Ala Ile Thr  Asp Lys Leu 
        995                 1000                 1005             


Lys Arg  Glu Trp Gly Leu Asn  Asp Val Trp Asn Thr  Ile Ile Tyr 
    1010                 1015                 1020             


Pro Arg  Phe Glu Arg Leu Asn  Gln Leu Thr Asn Ser  Asp Lys Phe 
    1025                 1030                 1035             


Gly Gln  Trp Glu Asn Lys Gln  Gly Lys Lys Val Phe  Gln Ile Glu 
    1040                 1045                 1050             


Met Pro  Leu Lys Leu Gln Lys  Gly Phe Asn Lys Lys  Arg Ile Asp 
    1055                 1060                 1065             


His Arg  His His Ala Met Asp  Ala Ile Val Ile Ala  Cys Ala Thr 
    1070                 1075                 1080             


Gln Ser  His Val Asn Tyr Leu  Asn Asn Glu Ser Ala  His Ser Asn 
    1085                 1090                 1095             


Ser Lys  Glu Lys Arg Tyr Tyr  Leu Arg Arg Lys Leu  Arg Arg Ile 
    1100                 1105                 1110             


Glu Ile  Leu Glu Lys Gln Glu  Leu Lys Asp Gly Val  Thr Ile Thr 
    1115                 1120                 1125             


Lys Lys  Ile Glu Val Ala Lys  Glu Phe Asn Lys Pro  Trp Pro Thr 
    1130                 1135                 1140             


Phe Thr  Gln Asp Ala His Glu  Val Leu Gln Ser Ile  Ile Val Ser 
    1145                 1150                 1155             


Phe Lys  Gln Asn Leu Arg Val  Val Asn Lys Ala Thr  Asn Arg Tyr 
    1160                 1165                 1170             


Ala Cys  Phe Val Asn Gly Lys  Lys Glu Ile Val Lys  Gln Ser Lys 
    1175                 1180                 1185             


Gly Glu  Ser Trp Ala Ile Arg  Lys Pro Met His Lys  Asp Thr Val 
    1190                 1195                 1200             


Ser Ala  Ala Val Ser Leu Arg  Lys Ile Lys Thr Val  Ser Leu Ser 
    1205                 1210                 1215             


Gln Ala  Ile Asp Asp Trp Thr  Asn Ile Val Asp Lys  Ser Leu Arg 
    1220                 1225                 1230             


Lys Glu  Ile Gly Leu Leu Tyr  Ser Lys Tyr Gly Gln  Asn Gly Ser 
    1235                 1240                 1245             


Lys Asn  Ile Ile Lys Tyr Phe  Lys Asp Arg Asp Asn  Lys His Asn 
    1250                 1255                 1260             


Gly Leu  Asp Val Ser Lys Val  Asn Val His Tyr Phe  Asp Asn Asp 
    1265                 1270                 1275             


Tyr Ala  Ala Ser Arg Val Ala  Leu Asp His Lys Phe  Asn Ser Ala 
    1280                 1285                 1290             


Lys Ile  Lys Ser Ile Thr Asp  Thr Gly Ile Gln Thr  Ile Leu Leu 
    1295                 1300                 1305             


Lys His  Leu Glu Ser Tyr Asn  Glu Ile Asn Gly Asp  Lys Ile Ile 
    1310                 1315                 1320             


Glu His  Pro Glu Leu Ala Phe  Ser Ala Asp Gly Leu  Asp Ile Leu 
    1325                 1330                 1335             


Asn Ala  Asn Ile Thr Lys Leu  Asn Asn Gly Lys Phe  His Lys Pro 
    1340                 1345                 1350             


Ile Lys  Lys Val Arg Lys Thr  Glu Thr Leu Gly Leu  Lys Phe Ile 
    1355                 1360                 1365             


Val Gly  Gln Thr Gly Asn Lys  Arg Lys Lys Tyr Val  Glu Ala Ala 
    1370                 1375                 1380             


Asp Gly  Thr Asn Leu Phe Phe  Ala Ile Tyr Val Ser  Val Asp Gly 
    1385                 1390                 1395             


Ile Arg  Ser Tyr Lys Thr Ile  Pro Leu Arg Glu Val  Ala Glu Arg 
    1400                 1405                 1410             


Leu Lys  Glu Gly Leu Ile Pro  Val Pro Glu Lys Asn  Asp Lys Asp 
    1415                 1420                 1425             


Asp His  Leu Leu Phe Trp Leu  Ser Pro Gly Asp Leu  Val Tyr Val 
    1430                 1435                 1440             


Pro Ser  Ile Glu Glu Glu Gly  Arg Ile Val Glu Ile  Glu Asn Ile 
    1445                 1450                 1455             


Lys Gln  Asp Arg Ile Tyr Lys  Val Val Ser Cys Thr  Glu Asn Glu 
    1460                 1465                 1470             


Cys His  Cys Ile Pro His Phe  Val Ala Ser Pro Ile  Val Lys Thr 
    1475                 1480                 1485             


Asp Glu  Leu Gly Ala Asn Asn  Lys Ala Gln Arg Ala  Trp Ser Gly 
    1490                 1495                 1500             


Glu Met  Ile Lys Glu Thr Cys  Ile Lys Ile Lys Met  Asn Arg Leu 
    1505                 1510                 1515             


Gly Gln  Ile Val Lys Ile 
    1520                 


<210>  1328
<211>  1526
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1328

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Leu Asn Ala Ala Asp Asp Asn Ser Leu Lys Tyr Ile Glu Ala 
            20                  25                  30          


Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile Leu Gly Asp Phe 
        35                  40                  45              


Asp Arg Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg Thr Arg Ile Arg 
    50                  55                  60                  


Gly Met Arg His Leu Arg Glu Arg Tyr Leu Leu Arg Arg Ala Arg Leu 
65                  70                  75                  80  


His Arg Ile Leu Asn Leu Leu Gly Phe Leu Pro Phe His Tyr Ala Gln 
                85                  90                  95      


Asp Ile Asp Arg Tyr Gly Asn Phe Ser Ala Asp Met Glu Pro Lys Ile 
            100                 105                 110         


Ala Trp Arg Lys Asn Leu Glu Gly Glu Leu Glu Phe Ile Phe Ile Glu 
        115                 120                 125             


Ser Phe Asn Glu Met Leu Glu Asp Phe Arg Lys Asn Gln Pro Glu Leu 
    130                 135                 140                 


Val Ala Asp Asn Lys Lys Val Pro Tyr Asp Trp Val Ile Tyr Tyr Leu 
145                 150                 155                 160 


Arg Lys Lys Ala Leu Glu Lys Lys Ile Ser Lys Glu Glu Leu Ala Trp 
                165                 170                 175     


Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly 
            180                 185                 190         


Glu Asp Asp Asp Glu Ser Met Lys Thr Ala Lys Thr Arg Gln Tyr Phe 
        195                 200                 205             


Asp Lys Gln Ile Ile Lys Asn Ile Ile Asp Thr Asn Gln Thr Tyr Lys 
    210                 215                 220                 


Gly Gln Arg Ile Leu Ile Val Glu Leu Gln Asn Gly Asp Lys Gly Lys 
225                 230                 235                 240 


Ile Phe Lys Lys Glu Ile Pro Asp Trp Ile Gly Gln Glu Lys Asn Ile 
                245                 250                 255     


Ile Val Thr Val Asp Ile Asp Lys Asp Gly Asn Asp Lys Tyr Glu Glu 
            260                 265                 270         


Ser Gly Ser Leu Ser Cys Arg Phe Lys Ile Pro Thr Asp Ala Glu Trp 
        275                 280                 285             


Glu Ser Glu Trp Lys Leu Ile Lys Met Lys Thr Gln Lys Asp Leu Asp 
    290                 295                 300                 


Glu Ser Asn Lys Thr Val Gly Thr Tyr Ile Tyr Asp Ser Ile Leu Gln 
305                 310                 315                 320 


Asn Pro Thr Gln Lys Val Arg Gly Lys Leu Val Arg Thr Ile Glu Arg 
                325                 330                 335     


Lys Tyr Tyr Lys Ala Glu Leu Lys Gln Ile Leu Glu Lys Gln Thr Glu 
            340                 345                 350         


Phe His Ser Glu Leu Gln Asp Lys Asp Leu Tyr Ile Glu Cys Leu Asn 
        355                 360                 365             


Glu Leu Tyr Ser Asn Asn Asp Thr His Arg Asn Ala Ile Thr Asp Arg 
    370                 375                 380                 


Asp Phe Val Tyr Leu Phe Met Asn Asp Ile Leu Phe Tyr Gln Arg Pro 
385                 390                 395                 400 


Leu Lys Ser Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Arg Asn 
                405                 410                 415     


Ile Tyr Ile Asp Lys Glu Thr Gly Glu Ile Lys Tyr Ser Pro Ile Lys 
            420                 425                 430         


Cys Ile Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Lys 
        435                 440                 445             


Phe Ile Ser Ser Ile Arg Ile Tyr Gln Arg Glu Ser Val Val Glu Val 
    450                 455                 460                 


Val Asp Lys Gln Leu Asp Leu Phe Ser Ser Gln Ser Thr Ser Val Gln 
465                 470                 475                 480 


Lys Leu Val Thr Asp Val Asp Val Thr Asp Lys Phe Leu Thr Cys Glu 
                485                 490                 495     


Asn Asp Tyr Val Ala Leu Phe Glu Trp Leu Asn Lys Lys Lys Ile Ile 
            500                 505                 510         


Asp Gln Lys Glu Phe Leu Lys Tyr Arg Pro Phe Gly Leu Lys Gly Asp 
        515                 520                 525             


Ile Asp Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys Ser Tyr Pro Cys 
    530                 535                 540                 


Asn Glu Thr His Ala Met Ile Leu Thr Tyr Phe Ala Lys Ala Gly Ile 
545                 550                 555                 560 


Ser Glu Asp Ile Leu Thr Lys Glu Met Glu Glu Ala Leu Trp His Ile 
                565                 570                 575     


Leu Tyr Ser Val Glu Asp Arg Gln Glu Ile Thr Lys Ala Leu Arg Thr 
            580                 585                 590         


Phe Ala Ser Lys Tyr His Leu Ser Asp Ser Phe Val Lys Val Phe Ser 
        595                 600                 605             


Asn Ile Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr Ser Ala Lys Ala 
    610                 615                 620                 


Ile Lys Lys Leu Leu Ser Leu Met Arg Met Gly Lys Tyr Trp Ser Glu 
625                 630                 635                 640 


Asp Ala Ile Asp Val Asn Thr Arg Glu Arg Ile Asp Lys Ile Val Thr 
                645                 650                 655     


Gly Glu Tyr Asp Glu Lys Ile Arg Asp Arg Val Arg Glu Lys Thr Ile 
            660                 665                 670         


Arg Leu Asn Asp Ile Arg His Phe Arg Ala Leu Pro Asp Trp Leu Ala 
        675                 680                 685             


Cys Tyr Val Val Tyr Asp Arg His Ser Glu Thr Arg Glu Ile Thr Glu 
    690                 695                 700                 


Trp Lys Ser Pro Glu Glu Ile Asp Glu Tyr Ile Arg Asn Phe Lys Gln 
705                 710                 715                 720 


His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu 
                725                 730                 735     


Arg Thr Val Arg Asp Ile Trp Lys Gln Val Gly Cys Ile Asp Glu Ile 
            740                 745                 750         


His Val Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Asp Lys Arg Lys 
        755                 760                 765             


Arg Ile Thr Ser Gln Ile Ile Glu Asn Glu Asn Ala Asn Leu Arg Val 
    770                 775                 780                 


Lys Ala Leu Leu Thr Glu Phe Val Asn Pro Glu Phe Glu Ile Glu Asn 
785                 790                 795                 800 


Val Arg Pro Tyr Ser Leu Gly Gln Gln Asp Ile Leu Arg Ile Tyr Glu 
                805                 810                 815     


Asp Gly Val Leu Asn Ser Val Ala Glu Val Pro Gly Asp Ile Ala Gly 
            820                 825                 830         


Ile Leu Lys Lys Phe Asn Glu Asn Asp Ala Lys Lys Arg Pro Thr Thr 
        835                 840                 845             


Ser Glu Ile Leu Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser 
    850                 855                 860                 


Pro Tyr Thr Gly Glu Met Ile Pro Leu Ser Lys Leu Phe Thr Ser Ala 
865                 870                 875                 880 


Tyr Glu Ile Glu His Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser 
                885                 890                 895     


Phe Ser Asn Lys Val Ile Cys Glu Ala Glu Val Asn Lys Leu Lys Asp 
            900                 905                 910         


Asn Ala Leu Gly Tyr Glu Phe Ile Lys Lys His His Gly Glu Lys Val 
        915                 920                 925             


Glu Leu Gly Phe Gly Lys Ile Val Glu Ile Phe Ser Val Ser Val Tyr 
    930                 935                 940                 


Glu Gln Phe Val Lys Asp Asn Tyr Ser Arg Asn Arg Gly Lys Met Lys 
945                 950                 955                 960 


Lys Leu Leu Met Asp Glu Ile Pro Glu Gln Phe Ile Glu Arg Gln Leu 
                965                 970                 975     


Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Thr Leu Leu Ser Asn 
            980                 985                 990         


Val Val Arg Glu Lys Ala Glu Gln  Glu Ala Val Ser Lys  Asn Val Ile 
        995                 1000                 1005             


Val Cys  Thr Gly Gly Val Thr  Asp Arg Leu Lys Lys  Asp Trp Gly 
    1010                 1015                 1020             


Ile Asn  Asp Val Trp Asn Lys  Ile Val Leu Pro Arg  Phe Leu Arg 
    1025                 1030                 1035             


Leu Asn  Glu Leu Thr Asn Ser  Thr Asn Phe Thr Ala  Lys Asn Ala 
    1040                 1045                 1050             


Ala Gly  Asn Glu Ile Pro Ala  Met Pro Leu Glu Leu  Gln Lys Gly 
    1055                 1060                 1065             


Phe Asn  Lys Lys Arg Ile Asp  His Arg His His Ala  Met Asp Ala 
    1070                 1075                 1080             


Ile Val  Ile Ala Cys Ala Asn  Arg Asn Ile Val Asn  Tyr Leu Asn 
    1085                 1090                 1095             


Asn Glu  Ser Ala Ser Arg Lys  Ala Lys Ile Ser Arg  His Asp Leu 
    1100                 1105                 1110             


Gln Ala  Ile Leu Cys Asp Lys  Val Arg Thr Asp Asp  Lys Gly Asn 
    1115                 1120                 1125             


Tyr Gln  Trp Arg Val Lys Lys  Pro Trp Asp Thr Phe  Thr Gln Asp 
    1130                 1135                 1140             


Val Tyr  Val Ala Leu Glu Asn  Ile Ile Val Ser Phe  Lys Gln Asn 
    1145                 1150                 1155             


Leu Arg  Val Ile Asn Lys Thr  Ser Asn Tyr Tyr Trp  His Tyr Glu 
    1160                 1165                 1170             


Asn Gly  Lys Lys Val Leu Val  Lys Gln Val Lys Gly  Asp Ser Trp 
    1175                 1180                 1185             


Ala Ile  Arg Lys Pro Met His  Lys Asp Thr Val Phe  Gly Glu Val 
    1190                 1195                 1200             


Asn Leu  Arg Lys Val Lys Ser  Val Ser Leu Asn Glu  Ala Leu Lys 
    1205                 1210                 1215             


Asn Pro  Gln Phe Ile Val Glu  Lys Asp Leu Lys Arg  Gln Leu Leu 
    1220                 1225                 1230             


Ser Leu  Leu Gln Gln Asn Phe  Asp Ile Lys Lys Ile  Lys Lys Tyr 
    1235                 1240                 1245             


Phe Glu  Glu His Lys Asp Ile  Trp Ser Asp Ile Ser  Leu Ser Lys 
    1250                 1255                 1260             


Met Asn  Ile Tyr Tyr Phe Thr  Lys Glu Thr Lys Asp  Arg Tyr Phe 
    1265                 1270                 1275             


Ala Thr  Arg Lys Pro Leu Asp  Ala Ser Phe Asp Lys  Lys Lys Ile 
    1280                 1285                 1290             


Glu Ser  Cys Ile Thr Asp Thr  Gly Ile Gln Gln Ile  Leu Leu Arg 
    1295                 1300                 1305             


His Leu  Glu Leu Lys Glu Asn  Asp Pro Glu Arg Ala  Phe Ser Pro 
    1310                 1315                 1320             


Asp Gly  Ile Asp Glu Met Asn  Arg Asn Ile Ile Gln  Leu Asn Lys 
    1325                 1330                 1335             


Gly Lys  Gly His His Pro Ile  Ala Lys Val Arg Val  Tyr Glu Lys 
    1340                 1345                 1350             


Ala Asp  Lys Phe Glu Val Gly  Gln Arg Gly Asn Lys  Ser Ser Lys 
    1355                 1360                 1365             


Phe Val  Glu Ala Ala Lys Gly  Thr Asn Leu Phe Phe  Ala Val Tyr 
    1370                 1375                 1380             


Glu Thr  Glu Tyr Pro Asp Glu  Lys Thr Asn Ser Ile  Val Arg Lys 
    1385                 1390                 1395             


Arg Asn  Tyr Ala Thr Ile Pro  Leu Asn Ile Ala Ile  Glu Arg Gln 
    1400                 1405                 1410             


Lys Arg  Gly Leu Ser Thr Ala  Pro Glu Asp Glu Lys  Gly Asn Val 
    1415                 1420                 1425             


Pro Ser  Phe Val Leu Ser Pro  Asn Asp Leu Val Tyr  Leu Pro Thr 
    1430                 1435                 1440             


Ser Glu  Asp Ile Val Asn Gly  His Ile Thr Gln Pro  Ile Asp Lys 
    1445                 1450                 1455             


Ser Arg  Ile Tyr Lys Met Val  Ser Ser Ser Gly Ser  Gln Cys Phe 
    1460                 1465                 1470             


Phe Ile  Lys His Asn Ile Ala  Asn Ile Ile Val Asp  Lys Tyr Glu 
    1475                 1480                 1485             


Phe Ser  Pro Leu Asn Lys Met  Glu Arg Ala Ile Ser  Asn Glu Met 
    1490                 1495                 1500             


Ile Lys  Glu Ile Cys Ile Pro  Val Lys Val Asp Arg  Leu Gly Asn 
    1505                 1510                 1515             


Ile Val  Lys Leu Gly Lys Ser  Glu 
    1520                 1525     


<210>  1329
<211>  1526
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1329

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Leu Asn Ala Ala Asp Asp Asn Ser Leu Lys Tyr Ile Glu Ala 
            20                  25                  30          


Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile Leu Gly Asp Phe 
        35                  40                  45              


Asp Arg Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg Thr Arg Ile Arg 
    50                  55                  60                  


Gly Met Arg His Leu Arg Glu Arg Tyr Leu Leu Arg Arg Ala Arg Leu 
65                  70                  75                  80  


His Arg Ile Leu Asn Leu Leu Gly Phe Leu Pro Phe His Tyr Ala Gln 
                85                  90                  95      


Asp Ile Asp Arg Tyr Gly Asn Phe Ser Ala Asp Met Glu Pro Lys Ile 
            100                 105                 110         


Ala Trp Cys Lys Asn Leu Glu Gly Glu Leu Glu Phe Ile Phe Ile Glu 
        115                 120                 125             


Ser Phe Asn Glu Met Leu Glu Asp Phe Arg Lys Asn Gln Pro Glu Leu 
    130                 135                 140                 


Val Ala Asp Asn Lys Lys Val Pro Tyr Asp Trp Val Ile Tyr Tyr Leu 
145                 150                 155                 160 


Arg Lys Lys Ala Leu Glu Lys Lys Ile Ser Lys Glu Glu Leu Ala Trp 
                165                 170                 175     


Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly 
            180                 185                 190         


Glu Asp Asp Asp Glu Ser Met Lys Thr Ala Lys Thr Arg Gln Tyr Phe 
        195                 200                 205             


Asp Lys Gln Ile Ile Lys Asn Ile Ile Asp Thr Asn Gln Thr Tyr Lys 
    210                 215                 220                 


Gly Gln Arg Ile Leu Ile Val Glu Leu Gln Asn Gly Asp Lys Gly Lys 
225                 230                 235                 240 


Ile Phe Lys Lys Glu Ile Pro Asp Trp Ile Gly Gln Glu Lys Asn Ile 
                245                 250                 255     


Ile Val Thr Val Asp Ile Asp Lys Asp Gly Asn Asp Lys Tyr Glu Glu 
            260                 265                 270         


Ser Gly Ser Leu Ser Cys Arg Phe Lys Ile Pro Thr Asp Ala Glu Trp 
        275                 280                 285             


Glu Ser Glu Trp Lys Leu Ile Lys Met Lys Thr Gln Lys Asp Leu Asp 
    290                 295                 300                 


Glu Ser Asn Lys Thr Val Gly Thr Tyr Ile Tyr Asp Ser Ile Leu Gln 
305                 310                 315                 320 


Asn Pro Thr Gln Lys Val Arg Gly Lys Leu Val Arg Thr Ile Glu Arg 
                325                 330                 335     


Lys Tyr Tyr Lys Ala Glu Leu Lys Gln Ile Leu Glu Lys Gln Thr Glu 
            340                 345                 350         


Phe His Ser Glu Leu Gln Asp Lys Asp Leu Tyr Ile Glu Cys Leu Asn 
        355                 360                 365             


Glu Leu Tyr Ser Asn Asn Asp Thr His Arg Asn Ala Ile Thr Asp Arg 
    370                 375                 380                 


Asp Phe Val Tyr Leu Phe Met Asn Asp Ile Leu Phe Tyr Gln Arg Pro 
385                 390                 395                 400 


Leu Lys Ser Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Arg Asn 
                405                 410                 415     


Ile Tyr Ile Asp Lys Glu Thr Gly Glu Ile Lys Tyr Leu Pro Ile Lys 
            420                 425                 430         


Cys Ile Ala Lys Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Lys 
        435                 440                 445             


Phe Ile Ser Ser Ile Arg Ile Tyr Gln Arg Glu Ser Val Val Glu Val 
    450                 455                 460                 


Val Asp Lys Gln Leu Asp Leu Phe Ser Ser Gln Ser Thr Ser Val Gln 
465                 470                 475                 480 


Lys Leu Val Thr Asp Val Asp Val Thr Asp Lys Phe Leu Thr Cys Glu 
                485                 490                 495     


Asn Asp Tyr Val Ala Leu Phe Glu Trp Leu Asn Lys Lys Lys Ile Ile 
            500                 505                 510         


Asp Gln Lys Glu Phe Leu Lys Tyr Arg Pro Phe Gly Leu Lys Gly Asp 
        515                 520                 525             


Ile Asp Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys Ser Tyr Pro Cys 
    530                 535                 540                 


Asn Glu Thr His Ala Met Ile Leu Thr Tyr Phe Ala Lys Ala Gly Ile 
545                 550                 555                 560 


Ser Glu Asp Ile Leu Thr Lys Glu Met Glu Glu Ala Leu Trp His Ile 
                565                 570                 575     


Leu Tyr Ser Val Glu Asp Arg Gln Glu Ile Thr Lys Ala Leu Arg Thr 
            580                 585                 590         


Phe Ala Ser Lys Tyr His Leu Ser Asp Ser Phe Val Lys Val Phe Ser 
        595                 600                 605             


Asn Ile Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr Ser Ala Lys Ala 
    610                 615                 620                 


Ile Lys Lys Leu Leu Ser Leu Met Arg Met Gly Lys Tyr Trp Ser Glu 
625                 630                 635                 640 


Asp Ala Ile Asp Val Asn Thr Arg Glu Arg Ile Asp Lys Ile Val Thr 
                645                 650                 655     


Gly Glu Tyr Asp Glu Lys Ile Arg Asp Arg Val Arg Glu Lys Thr Ile 
            660                 665                 670         


Arg Leu Asn Asp Ile Arg His Phe Arg Ala Leu Pro Asp Trp Leu Ala 
        675                 680                 685             


Cys Tyr Val Val Tyr Asp Arg His Ser Glu Thr Arg Glu Ile Thr Glu 
    690                 695                 700                 


Trp Lys Ser Pro Glu Glu Ile Asp Glu Tyr Ile Arg Asn Phe Lys Gln 
705                 710                 715                 720 


His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Thr Glu Thr Leu 
                725                 730                 735     


Arg Thr Val Arg Asp Ile Trp Lys Gln Val Gly Cys Ile Asp Glu Ile 
            740                 745                 750         


His Val Glu Leu Gly Arg Glu Met Lys Asn Pro Ala Asp Lys Arg Lys 
        755                 760                 765             


Arg Ile Thr Ser Gln Ile Ile Glu Asn Glu Asn Ala Asn Leu Arg Val 
    770                 775                 780                 


Lys Ala Leu Leu Thr Glu Phe Val Asn Pro Glu Phe Glu Ile Glu Asn 
785                 790                 795                 800 


Val Arg Pro Tyr Ser Leu Ser Gln Gln Asp Ile Leu Arg Ile Tyr Glu 
                805                 810                 815     


Asp Gly Val Leu Asn Ser Val Ala Glu Val Pro Gly Asp Ile Ala Gly 
            820                 825                 830         


Ile Leu Lys Lys Phe Asn Glu Asn Asp Ala Lys Lys Arg Pro Thr Thr 
        835                 840                 845             


Ser Glu Ile Leu Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser 
    850                 855                 860                 


Pro Tyr Thr Gly Glu Met Ile Pro Leu Ser Lys Leu Phe Thr Ser Ala 
865                 870                 875                 880 


Tyr Glu Ile Glu His Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser 
                885                 890                 895     


Phe Ser Asn Lys Val Ile Cys Glu Ala Glu Val Asn Lys Leu Lys Asp 
            900                 905                 910         


Asn Ala Leu Gly Tyr Glu Phe Ile Lys Lys His His Gly Glu Lys Val 
        915                 920                 925             


Glu Leu Gly Phe Gly Lys Ile Val Glu Ile Phe Ser Val Ser Val Tyr 
    930                 935                 940                 


Glu Gln Phe Val Lys Asp Asn Tyr Ser Arg Asn Arg Gly Lys Met Lys 
945                 950                 955                 960 


Lys Leu Leu Met Asp Glu Ile Pro Glu Gln Phe Ile Glu Arg Gln Leu 
                965                 970                 975     


Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Thr Leu Leu Ser Asn 
            980                 985                 990         


Val Val Arg Glu Lys Ala Glu Gln  Glu Ala Val Ser Lys  Asn Val Ile 
        995                 1000                 1005             


Val Cys  Thr Gly Gly Val Thr  Asp Arg Leu Lys Lys  Asp Trp Gly 
    1010                 1015                 1020             


Ile Asn  Asp Val Trp Asn Lys  Ile Val Leu Pro Arg  Phe Leu Arg 
    1025                 1030                 1035             


Leu Asn  Glu Leu Thr Asn Ser  Thr Asn Phe Thr Ala  Lys Asn Ala 
    1040                 1045                 1050             


Ala Gly  Asn Glu Ile Pro Ala  Met Pro Leu Glu Leu  Gln Lys Gly 
    1055                 1060                 1065             


Phe Asn  Lys Lys Arg Ile Asp  His Arg His His Ala  Met Asp Ala 
    1070                 1075                 1080             


Ile Val  Ile Ala Cys Ala Asn  Arg Asn Ile Val Asn  Tyr Leu Asn 
    1085                 1090                 1095             


Asn Glu  Ser Ala Ser Arg Lys  Ala Lys Ile Ser Arg  His Asp Leu 
    1100                 1105                 1110             


Gln Ala  Ile Leu Cys Asp Lys  Val Arg Thr Asp Asp  Lys Gly Asn 
    1115                 1120                 1125             


Tyr Gln  Trp Arg Val Lys Lys  Pro Trp Asp Thr Phe  Thr Gln Asp 
    1130                 1135                 1140             


Val Tyr  Val Ala Leu Glu Asn  Ile Ile Val Ser Phe  Lys Gln Asn 
    1145                 1150                 1155             


Leu Arg  Val Ile Asn Lys Thr  Ser Asn Tyr Tyr Trp  His Tyr Glu 
    1160                 1165                 1170             


Asn Gly  Lys Lys Val Leu Val  Lys Gln Val Lys Gly  Asp Ser Trp 
    1175                 1180                 1185             


Ala Ile  Arg Lys Pro Met His  Lys Asp Thr Val Phe  Gly Glu Val 
    1190                 1195                 1200             


Asn Leu  Arg Lys Val Lys Ser  Val Ser Leu Asn Glu  Ala Leu Lys 
    1205                 1210                 1215             


Asn Pro  Gln Phe Ile Val Glu  Lys Asp Leu Lys Arg  Gln Leu Leu 
    1220                 1225                 1230             


Ser Leu  Leu Gln Gln Asn Phe  Asp Ile Lys Lys Ile  Lys Lys Tyr 
    1235                 1240                 1245             


Phe Glu  Glu His Lys Asp Ile  Trp Ser Asp Ile Ser  Leu Ser Lys 
    1250                 1255                 1260             


Met Asn  Ile Tyr Tyr Phe Thr  Lys Glu Thr Lys Asp  Arg Tyr Phe 
    1265                 1270                 1275             


Ala Thr  Arg Lys Pro Leu Asp  Ala Ser Phe Asp Lys  Lys Lys Ile 
    1280                 1285                 1290             


Glu Ser  Cys Ile Thr Asp Thr  Gly Ile Gln Gln Ile  Leu Leu Arg 
    1295                 1300                 1305             


His Leu  Glu Leu Lys Glu Asn  Asp Pro Glu Arg Ala  Phe Ser Pro 
    1310                 1315                 1320             


Asp Gly  Ile Asp Glu Met Asn  Arg Asn Ile Ile Gln  Leu Asn Lys 
    1325                 1330                 1335             


Gly Lys  Gly His His Pro Ile  Ala Lys Val Arg Val  Tyr Glu Lys 
    1340                 1345                 1350             


Ala Asp  Lys Phe Glu Val Gly  Gln Arg Gly Asn Lys  Ser Ser Lys 
    1355                 1360                 1365             


Phe Val  Glu Ala Ala Lys Gly  Thr Asn Leu Phe Phe  Ala Val Tyr 
    1370                 1375                 1380             


Glu Thr  Glu Tyr Pro Asp Glu  Lys Thr Asn Ser Ile  Val Arg Lys 
    1385                 1390                 1395             


Arg Asn  Tyr Ala Thr Ile Pro  Leu Asn Ile Ala Ile  Glu Arg Gln 
    1400                 1405                 1410             


Lys Arg  Gly Leu Ser Thr Ala  Pro Glu Asp Glu Lys  Gly Asn Val 
    1415                 1420                 1425             


Pro Ser  Phe Val Leu Ser Pro  Asn Asp Leu Val Tyr  Leu Pro Thr 
    1430                 1435                 1440             


Ser Glu  Asp Ile Val Asn Gly  His Ile Thr Gln Pro  Ile Asp Lys 
    1445                 1450                 1455             


Ser Arg  Ile Tyr Lys Met Val  Ser Ser Ser Gly Ser  Gln Cys Phe 
    1460                 1465                 1470             


Phe Ile  Lys His Asn Ile Ala  Asn Ile Ile Val Asp  Lys Tyr Glu 
    1475                 1480                 1485             


Phe Ser  Pro Leu Asn Lys Met  Glu Arg Ala Ile Ser  Asn Glu Met 
    1490                 1495                 1500             


Ile Lys  Glu Ile Cys Ile Pro  Val Lys Val Asp Arg  Leu Gly Asn 
    1505                 1510                 1515             


Ile Val  Lys Leu Gly Lys Ser  Glu 
    1520                 1525     


<210>  1330
<211>  1527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1330

Met Ala Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Ile Val Glu Lys Asp Gly Asn Asp Phe Ser Leu Val Asp Lys Gly 
            20                  25                  30          


Val Arg Ile Phe Ser Glu Gly Val Lys Ser Glu Lys Gly Ile Glu Ser 
        35                  40                  45              


Ser Arg Ala Ala Glu Arg Thr Gly Tyr Arg Ser Ala Arg Lys Ile Lys 
    50                  55                  60                  


Tyr Arg Arg Lys Leu Arg Lys Tyr Glu Thr Leu Lys Val Leu Ser Leu 
65                  70                  75                  80  


Asn Gly Met Cys Pro Leu Ser Ile Glu Glu Val Glu Glu Trp Lys Lys 
                85                  90                  95      


Ser Gly Phe Lys Glu Tyr Pro Leu Asn Pro Glu Phe Leu Glu Trp Leu 
            100                 105                 110         


Arg Thr Ser Asp Asp Glu Asn Ile Asn Pro Tyr Phe Phe Arg Asp Lys 
        115                 120                 125             


Ala Ser Lys Gln Lys Val Thr Leu Phe Glu Val Gly Arg Ala Phe Tyr 
    130                 135                 140                 


His Ile Ala Gln Arg Arg Gly Phe Leu Ser Asn Arg Leu Asp Gln Ser 
145                 150                 155                 160 


Ala Glu Gly Ile Leu Glu Glu His Cys Pro Lys Ile Glu Ala Leu Val 
                165                 170                 175     


Glu Asp Leu Asn Ser Thr Asp Glu Ile Leu Thr Ser Leu Lys Asp Tyr 
            180                 185                 190         


Phe Phe Asp Thr Gly Ile Leu Asp Ala Asn Glu Lys Gly Gly Phe Ala 
        195                 200                 205             


Lys Asp Leu Asp Glu Gly Asp Lys Lys Leu Val Ser Leu Tyr Arg Ser 
    210                 215                 220                 


Ile Gln Ala Ile Val Lys Lys Asn Asp Thr Asp Phe Glu Lys Gly Lys 
225                 230                 235                 240 


Ala Glu Ile Ile Glu Arg Leu Asn Lys Lys Asp Val Leu Gly Lys Val 
                245                 250                 255     


Lys Gly Lys Ile Lys Asp Ile Ser Gln Ala Met Leu Glu Gly Asn Phe 
            260                 265                 270         


Lys Thr Leu Gly Gln Tyr Phe Phe Ser Leu Tyr Asn Lys Glu Arg Ile 
        275                 280                 285             


Arg Asn Gln Tyr Thr Ser Arg Glu Asp His Tyr Leu Glu Glu Phe Lys 
    290                 295                 300                 


Ile Ile Cys Lys Phe Gln Gly Ile Gly Ser Ile Asn Gln Asn Glu Lys 
305                 310                 315                 320 


Leu Pro Glu Lys Lys Phe Glu Gly Leu Ala Lys Glu Leu Tyr Lys Ala 
                325                 330                 335     


Ile Phe Phe Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Ile Gly Lys 
            340                 345                 350         


Cys Ser Phe Glu Lys Ser Lys Ser Arg Cys Ala Ile Ser His Pro Asp 
        355                 360                 365             


Phe Glu Glu Lys Arg Met Trp Glu Tyr Leu Asn Thr Ile Lys Ile Gly 
    370                 375                 380                 


Thr Gln Ser Glu Lys Val Leu Arg Phe Leu Ser Gln Glu Glu Lys Leu 
385                 390                 395                 400 


Asn Leu Ile Pro Lys Phe Leu Arg Lys Lys Asp Asn Phe Asn Phe Glu 
                405                 410                 415     


Asp Leu Ala Lys Glu Leu Ile Glu Lys Gly Ala Thr Phe Gly Phe Tyr 
            420                 425                 430         


Lys Ser Ser Lys Arg Asp Ser Phe His Tyr Trp Phe Asn Tyr Lys Pro 
        435                 440                 445             


Thr Asp Thr Val Ser Gly Cys Pro Val Ser Ala Ser Leu Lys Ser Val 
    450                 455                 460                 


Val Gly Glu Asp Trp Lys Thr Lys Ser Phe Ile Tyr Lys Thr Thr Asn 
465                 470                 475                 480 


Ser Lys Gly Ile Glu Val Ser Arg Thr Val Asn Tyr Lys Asp Leu Trp 
                485                 490                 495     


His Leu Leu Ser Val Ser Thr Ser Asp Ile Tyr Leu Tyr Glu Tyr Ala 
            500                 505                 510         


Lys Asn Lys Leu Gly Leu Glu Asp Lys Asn Ala Lys Ala Phe Ser Lys 
        515                 520                 525             


Ile Lys Leu Lys Lys Asp Phe Gly Asn Leu Ser Leu Asn Ala Ile Asn 
    530                 535                 540                 


Lys Ile Leu Pro Tyr Met Arg Glu Gly Leu Leu Tyr Ser His Ala Val 
545                 550                 555                 560 


Phe Met Ala Asn Ile Glu Ser Ile Val Asp Glu Val Ile Trp Asn Asp 
                565                 570                 575     


Leu Gly Gln Arg Lys Tyr Ile Gln Asp Lys Ile Ala Ala Ile Ile Ala 
            580                 585                 590         


Asn Asn Ile Val Asp Asn Gly Leu Leu Glu Val Val Asn Gly Leu Ile 
        595                 600                 605             


Lys Glu Cys Lys Val Asp Gly Ala Tyr Tyr Ser Lys Glu Ala Ala Asp 
    610                 615                 620                 


Thr Tyr Arg Lys Asp Leu Ser Lys Lys Leu Val Ser Phe Phe Lys Phe 
625                 630                 635                 640 


His His Ile Asp Asp Gly Ile Lys Gln Glu Thr Ile Leu Asn Glu Leu 
                645                 650                 655     


Phe Pro Val Phe Ile Glu Gln Leu Lys Ala His Glu Phe Ile Lys Val 
            660                 665                 670         


Lys Arg Leu Asp Glu Lys Val Leu Glu Phe Leu Lys Gly Glu Asn Gln 
        675                 680                 685             


Asp Gly Glu Val Phe Cys Asn Asn Ala Lys Gln Leu Lys Lys Leu Tyr 
    690                 695                 700                 


His Pro Ser Asp Ile Glu Val Phe Lys Lys Gln Ile Val Lys Asp Lys 
705                 710                 715                 720 


Asp Gly Thr Glu Thr Gly Gln Leu Ile Leu Gly Ser Pro Leu Thr Ser 
                725                 730                 735     


Ser Ile Lys Asn Pro Met Ala Met Arg Ala Leu His Gln Leu Arg Lys 
            740                 745                 750         


Val Leu Asn Thr Leu Ile Val Glu Glu Gln Val Asp Ser Ser Thr Arg 
        755                 760                 765             


Ile His Ile Glu Met Ala Arg Glu Leu Asn Asp Ala Asn Lys Arg Lys 
    770                 775                 780                 


Gly Ile Gln Asp Tyr Gln Asn Glu Asn Lys Lys Glu Arg Glu Lys Tyr 
785                 790                 795                 800 


Arg Asp Glu Ile Lys Lys Leu Tyr Leu Ala Glu Cys Asn Lys Glu Ile 
                805                 810                 815     


Glu Pro Thr Val Asp Asp Leu Leu Arg Tyr Gln Leu Trp Ile Glu Gln 
            820                 825                 830         


Asp Arg Lys Glu Ile Tyr Glu Asn Gly Asn Ser Ile Ser Ile Cys Asp 
        835                 840                 845             


Ile Ile Gly Asp Asn Pro Ala Tyr Asp Ile Glu His Thr Val Pro Arg 
    850                 855                 860                 


Ser Ile Ser Gln Asp Asn Ser Gln Met Asn Lys Thr Leu Cys Ser Gln 
865                 870                 875                 880 


Lys Phe Asn Arg Asp Val Lys Lys Gln Lys Met Pro Phe Glu Leu Gly 
                885                 890                 895     


Asn His Leu Glu Ile Leu Pro Arg Ile Ala His Trp Lys Glu Glu Ala 
            900                 905                 910         


Asp Asn Leu Ser Arg Glu Ile Glu Met Ile Ile Arg Ser Thr Lys Thr 
        915                 920                 925             


Ala Ala Thr Lys Glu Ala Lys Asp Lys Lys Ile Arg Arg Arg His Tyr 
    930                 935                 940                 


Leu Thr Leu Arg Arg Asp Tyr Leu Gln Gly Lys Tyr Asp Arg Phe Ile 
945                 950                 955                 960 


Trp Thr Glu Pro Lys Val Gly Phe Lys Asn Ser Gln Ile Pro Asp Thr 
                965                 970                 975     


Gly Ile Ile Thr Arg Tyr Ala Gln Ala Tyr Leu Lys Ser Tyr Phe Asn 
            980                 985                 990         


Arg Val Glu Ser Val Lys Gly Gly  Met Val Ala Glu Phe  Arg Lys Ile 
        995                 1000                 1005             


Trp Gly  Ile Gln Glu Ser Tyr  Gln Glu Asn Gly Lys  Lys Tyr Phe 
    1010                 1015                 1020             


Lys Glu  Lys Asp Arg Ser Lys  His Thr His His Thr  Ile Asp Ala 
    1025                 1030                 1035             


Ile Thr  Ile Ala Cys Met Thr  Lys Asp Lys Tyr Asp  Val Leu Ala 
    1040                 1045                 1050             


His Ala  Trp Thr Leu Glu Asp  Lys Glu Gln Ile Thr  Glu Ala Arg 
    1055                 1060                 1065             


Lys Ile  Val Ala Glu Ser Lys  Pro Trp Lys Thr Phe  Lys Glu Asp 
    1070                 1075                 1080             


Leu Lys  Lys Ile Glu Glu Gly  Ile Leu Val Ser His  Tyr Thr Pro 
    1085                 1090                 1095             


Asp Asn  Val Lys Lys Gln Ser  Lys Lys Ile Val Arg  Val Arg Gly 
    1100                 1105                 1110             


Arg Lys  Gln Tyr Val Ala Glu  Ile Glu Arg Asp Val  Asn Gly Lys 
    1115                 1120                 1125             


Ala Val  Ala Lys Lys Asp Ile  Ser Gly Lys Val Ile  Tyr Lys Leu 
    1130                 1135                 1140             


Asp Lys  Asp Gly Lys Lys Leu  Pro Arg Leu Gln Gln  Gly Asp Thr 
    1145                 1150                 1155             


Ile Arg  Gly Ser Leu His Gln  Asp Ser Ile Tyr Gly  Ala Ile Lys 
    1160                 1165                 1170             


Asn Pro  Leu Lys Thr Glu Glu  Ile Arg Tyr Val Ile  Arg Lys Asp 
    1175                 1180                 1185             


Leu Glu  Ser Ile Lys Ala Ser  Asp Val Asp Asn Ile  Val Asp Glu 
    1190                 1195                 1200             


Met Val  Lys Glu Lys Val Lys  Glu Ala Ile Ala Ser  Lys Val Leu 
    1205                 1210                 1215             


Ile Leu  Ser Ser Asn Pro Gln  Gln Lys Asn Lys Ile  Asp Gly Lys 
    1220                 1225                 1230             


Val Trp  Met Asn Glu Glu Lys  Gly Ile Pro Ile Asn  Lys Val Arg 
    1235                 1240                 1245             


Leu Tyr  Ala Asn Ser Val Lys  Asn Pro Leu Glu Ile  Lys Glu His 
    1250                 1255                 1260             


Ser Ser  Leu Ser Lys Ser Arg  His Glu His Lys Gln  Lys Val Tyr 
    1265                 1270                 1275             


Gly Gln  Asn Asp Glu Asn Phe  Ala Met Ala Ile Tyr  Glu Gly Leu 
    1280                 1285                 1290             


Asp Lys  Lys Gly Lys Thr Glu  Arg Ala Phe Glu Leu  Ile Asn Tyr 
    1295                 1300                 1305             


Met Asp  Ala Gly Tyr Tyr Tyr  Lys Leu Lys Asn Lys  Asn Glu Arg 
    1310                 1315                 1320             


Lys Lys  Asp Ile Ile Ser Leu  Val Pro Glu Lys His  Ile Glu Thr 
    1325                 1330                 1335             


Gly Phe  Asn Leu Leu Arg Ser  Asn Lys Ile Pro Ile  Ile Leu Lys 
    1340                 1345                 1350             


Lys Gly  Leu Asn Val Ile Leu  Tyr Lys Asn Glu Lys  Glu Glu Ile 
    1355                 1360                 1365             


Asp Phe  Asn Asn Pro Asn Ser  Ile Ile Lys Arg Leu  Tyr Lys Ile 
    1370                 1375                 1380             


Ser Gly  Leu Asp Lys Asp Gly  Ile Lys Leu Met Tyr  Cys Ile Ser 
    1385                 1390                 1395             


Ala Lys  Gly Ser Thr Glu Asn  Ile Ala Tyr Met Asn  Asp Val Ile 
    1400                 1405                 1410             


Asn Lys  Gln Lys Leu Phe Glu  Leu Lys Ser Glu Met  Glu Lys Leu 
    1415                 1420                 1425             


Gly Leu  Trp Asp Lys Val Phe  Ser Asp Phe Lys Asn  Asp Asn Leu 
    1430                 1435                 1440             


Lys Lys  Ser Asp Phe Phe Lys  Lys Leu Asn Asp Leu  Leu Asn Lys 
    1445                 1450                 1455             


His Tyr  Phe Glu Asn Asn Val  Cys Asp Ser Ile Gly  Ser Ile Lys 
    1460                 1465                 1470             


Lys Val  Lys Ile Lys Glu Ser  Gly Leu Thr Thr Pro  Lys Gly Gly 
    1475                 1480                 1485             


Asp Val  Ile Asp Asn Phe Lys  Asp Phe Pro Tyr Ile  Lys Phe Lys 
    1490                 1495                 1500             


Val Ser  Asn Phe Asn Ala Leu  Ile Glu Gly Ile Asp  Phe Lys Ile 
    1505                 1510                 1515             


Ser Pro  Thr Gly Lys Phe Val  Gln Ile 
    1520                 1525         


<210>  1331
<211>  1528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1331

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Glu Gln Asp Phe Lys Asn Lys Gln Gly Asn Ile Met Gly 
            20                  25                  30          


Met Gly Ser Arg Val Ile Pro Met Ser Gln Glu Ile Leu Gly Glu Phe 
        35                  40                  45              


Ala Arg Gly Asn Thr Lys Ser Glu Thr Ala Glu Arg Thr Arg Leu Arg 
    50                  55                  60                  


Ser Val Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Ile Leu Gly Phe Leu Pro Lys His Tyr Val Asn 
                85                  90                  95      


Gln Ile Asp Phe Gln Glu Arg Leu Gly Lys Phe Phe Glu Glu Ser Glu 
            100                 105                 110         


Pro Lys Leu Ala Tyr Asp Thr Gly Asp Phe Leu Phe Lys Pro Ser Phe 
        115                 120                 125             


Leu Glu Met Leu Glu Glu Phe Lys Ile His Gln Pro Ser Phe Val Glu 
    130                 135                 140                 


Gly Asp Lys Lys Val Pro Tyr Asp Trp Thr Ile Tyr Phe Leu Arg Lys 
145                 150                 155                 160 


Lys Ala Leu Thr Glu Arg Ile Glu Lys Glu Glu Leu Ala Trp Val Ile 
                165                 170                 175     


Leu Ser Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Asp 
            180                 185                 190         


Glu Glu Glu Lys Pro Asn Lys Leu Glu Glu Phe Tyr Ser Leu Lys Ile 
        195                 200                 205             


Val Asp Ile Lys Val Asp Glu Ser Pro Asn Lys Lys Gly Asp Thr Trp 
    210                 215                 220                 


Tyr Ser Leu Thr Leu Glu Asn Gly Trp Ile Tyr Arg Arg Ser Ser Lys 
225                 230                 235                 240 


Val Pro Leu Asp Asp Trp Leu Gly Lys Thr Arg Asp Phe Ile Val Thr 
                245                 250                 255     


Thr Asp Ile Asp Glu Glu Gly Asn Pro Lys Leu Asp Lys Glu Gly Asn 
            260                 265                 270         


Pro Lys Arg Ser Phe Arg Ala Pro Ser Glu Asp Asp Trp Gly Leu Arg 
        275                 280                 285             


Lys Lys Lys Thr Glu Lys Glu Ile Leu Asp Ser Ser Lys Thr Val Gly 
    290                 295                 300                 


His Phe Ile Phe Glu Asn Leu Leu Ala Asp Pro Thr Gln Lys Ile Lys 
305                 310                 315                 320 


Gly Arg Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Asp Glu Leu 
                325                 330                 335     


Lys Gln Ile Leu Val Ser Gln Asn Arg Phe His Pro Glu Leu Ser Asp 
            340                 345                 350         


Ala Asp Met Met Ala Asp Cys Val Arg Glu Leu Tyr Lys His Asn His 
        355                 360                 365             


Ser His Gln Asn Phe Leu Ala Ser Lys Asp Ile Lys His Leu Leu Val 
    370                 375                 380                 


Asp Asp Ile Ile Phe Tyr Gln Arg Pro Leu Arg Ser Gln Lys Ser Thr 
385                 390                 395                 400 


Ile Gly Asn Cys Pro Leu Glu Phe Lys Thr Phe Lys Lys Asp Gly Ile 
                405                 410                 415     


Glu Ile Lys Val Pro Tyr Lys Val Ala Pro Lys Ser His Pro Leu Phe 
            420                 425                 430         


Gln Glu Phe Arg Leu Leu Gln Trp Val Ser Ser Leu Arg Ile Ile Arg 
        435                 440                 445             


Lys Ser Asp Asp Ser Asp Val Thr Arg Glu Phe Leu Gly Thr Gln Asp 
    450                 455                 460                 


Lys Thr Lys Gln Leu Leu Asp Tyr Leu Met Thr Gln Ser Glu Met Asp 
465                 470                 475                 480 


Cys Asn Lys Leu Met Thr Phe Leu Leu Thr Gln Glu Gly Tyr Lys Arg 
                485                 490                 495     


Lys Asp Leu Lys Ser Glu Val Gly Lys Phe Arg Trp Asn Tyr Val Phe 
            500                 505                 510         


Asp Ser Glu Thr Gly Glu Ser Lys Thr Tyr Pro Gly Asn Ser Thr Arg 
        515                 520                 525             


Ala Glu Phe Leu Lys Arg Leu Gly Lys Val Glu Gly Phe Asp Phe Ser 
    530                 535                 540                 


Lys Tyr Thr Glu Glu Phe Glu Glu Ser Leu Trp His Leu Val Tyr Ser 
545                 550                 555                 560 


Val Thr Asp Lys Val Glu Phe Glu Lys Ala Leu Arg Lys Phe Ser Arg 
                565                 570                 575     


Lys Asn Gly Ile Glu Ile Asn Ser Phe Val Glu Asn Leu Lys Lys Phe 
            580                 585                 590         


Pro Pro Tyr Lys Ser Glu Tyr Ala Ser Tyr Ser Leu Lys Ala Ile Lys 
        595                 600                 605             


Lys Leu Leu Ala Leu Met Arg Phe Gly Glu Asn Trp Lys Glu Asp Ser 
    610                 615                 620                 


Ile Asp Ser Ile Ser Lys His Arg Ile Glu Lys Ile Leu Thr Gly Glu 
625                 630                 635                 640 


Tyr Asp Pro Glu Ile Lys Asp Lys Val Arg Glu Ile Ser Arg Ala Phe 
                645                 650                 655     


Asp Glu Val Glu Asp Phe Gln Gly Leu Pro Val Trp Phe Ala Asn Tyr 
            660                 665                 670         


Val Val Tyr Gly Arg His Ser Glu Ala Ser Glu Leu Ile Lys Trp Thr 
        675                 680                 685             


Ser Ser Asp Glu Met Ala Asp Phe Ile Lys Glu Phe Lys Gln His Ser 
    690                 695                 700                 


Leu Arg Asn Pro Ile Val Glu Gln Val Val Leu Glu Thr Leu Arg Val 
705                 710                 715                 720 


Val Lys Asp Ile Trp Asp Lys Tyr Gly His Gly Gln Lys Asp Phe Phe 
                725                 730                 735     


Asp Glu Ile His Val Glu Leu Gly Arg Glu Leu Lys Asn Pro Ala Glu 
            740                 745                 750         


Gln Arg Ala Arg Leu Thr Thr Gln Ile Ser Ala Asn Glu Asn Thr Asn 
        755                 760                 765             


Leu Arg Ile Lys Ser Leu Leu Val Glu Leu Leu His Asp Pro Gly Val 
    770                 775                 780                 


Glu Asn Val Arg Pro Tyr Ser Pro Met Gln Gln Glu Ile Leu Lys Ile 
785                 790                 795                 800 


Tyr Glu Glu Gly Val Leu Lys Ser Ser Phe Glu Val Pro Asp Asp Ile 
                805                 810                 815     


Leu Lys Ile Ser Lys Thr Ala Gln Pro Ser Ser Ser Asp Leu Lys Arg 
            820                 825                 830         


Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly Lys 
        835                 840                 845             


Ile Ile Pro Leu Asn Lys Leu Phe Thr Pro Ala Tyr Glu Val Glu His 
    850                 855                 860                 


Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val 
865                 870                 875                 880 


Ile Cys Glu Ala Ala Val Asn Lys Leu Lys Asp Asn Arg Leu Gly Met 
                885                 890                 895     


Glu Phe Ile Lys Cys Phe His Gly Gln Arg Val Ser Leu Gly Phe Gly 
            900                 905                 910         


Glu Glu Val Glu Ile Phe Ser Glu Ser Ala Tyr Lys Ala Phe Ile Gln 
        915                 920                 925             


Asp Asn Phe Pro Lys Asn Pro Ala Lys Lys Arg Lys Leu Leu Met Asp 
    930                 935                 940                 


Glu Leu Pro Glu Gln Met Val Glu Arg Gln Met Asn Asp Thr Arg Tyr 
945                 950                 955                 960 


Ile Ser Lys Phe Val Thr Ser Ile Leu Ser Asn Ile Val Arg Ser Glu 
                965                 970                 975     


Glu Asn Asp Thr Gly Phe Asn Ser Lys Asn Ile Ile Pro Gly Asn Gly 
            980                 985                 990         


Lys Ile Thr Thr Ala Leu Lys Gln  Asp Trp Gly Leu Asn  Asp Val Trp 
        995                 1000                 1005             


Asn Glu  Leu Ile Leu Pro Arg  Phe Glu Arg Met Asn  Gln Leu Thr 
    1010                 1015                 1020             


Gly Thr  Thr Val Phe Thr Ala  Trp Asn Glu Asn His  Gln Lys Phe 
    1025                 1030                 1035             


Leu Pro  Thr Val Pro Leu Glu  Tyr Ala Lys Gly Phe  Gln Lys Lys 
    1040                 1045                 1050             


Arg Ile  Asp His Arg His His  Ala Met Asp Ala Leu  Val Ile Ala 
    1055                 1060                 1065             


Cys Ala  Thr Arg Asp His Val  Asn Tyr Leu Asn Asn  Glu His Ala 
    1070                 1075                 1080             


Leu Gly  Lys Asn Tyr Lys Gly  Lys Glu Glu Lys Gln  Ser Arg Arg 
    1085                 1090                 1095             


Phe Asp  Leu Arg Gln Lys Leu  Cys Leu Lys Lys Lys  Asn Gly His 
    1100                 1105                 1110             


Ser Gln  Glu Gln Tyr Glu Trp  Ile Phe Ser Lys Pro  Trp Asp Ser 
    1115                 1120                 1125             


Phe Thr  Gln Asp Ala Lys Lys  Asn Leu Glu Arg Ile  Ile Val Ser 
    1130                 1135                 1140             


Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys Thr Ser  Asn Lys Tyr 
    1145                 1150                 1155             


Leu Ser  Tyr Lys Asp Glu Glu  Gly Asn Leu Arg Leu  Asp Ser Gln 
    1160                 1165                 1170             


Gly Lys  Pro Val Lys Ala Leu  Thr Arg Gln Ile Lys  Gly Asp Ala 
    1175                 1180                 1185             


Trp Ala  Ile Arg Lys Ser Ile  His Lys Asp Thr Val  Ser Gly Leu 
    1190                 1195                 1200             


Val Lys  Leu Arg Arg Val Lys  Met Val Asn Leu Ser  Val Ala Leu 
    1205                 1210                 1215             


Asp Asn  Pro Glu Asn Ile Leu  Asn Lys Lys Leu Lys  Lys Lys Ile 
    1220                 1225                 1230             


Ala Glu  Leu Val Lys Leu Asn  Tyr Asp Asn Lys Leu  Leu Leu Lys 
    1235                 1240                 1245             


Tyr Phe  Arg Asp Arg Asp Tyr  Met Phe Asp Gly Arg  Glu Val Ser 
    1250                 1255                 1260             


Lys Leu  Asp Val Tyr Tyr Phe  Glu Glu Gly Leu Ala  Ala Ser Arg 
    1265                 1270                 1275             


Val Ser  Leu Asp Thr Ser Phe  Ser Asp Lys Lys Ile  Gln Ser Ile 
    1280                 1285                 1290             


Thr Asp  Thr Gly Ile Arg Lys  Ile Leu Leu Ala His  Leu Ala Asn 
    1295                 1300                 1305             


Tyr His  Gly Arg Leu Asp Glu  Lys Gly Lys Val Ile  Pro Pro Glu 
    1310                 1315                 1320             


Thr Leu  Ala Phe Thr Pro Glu  Gly Ile Glu Glu Leu  Asn Lys Asn 
    1325                 1330                 1335             


Ile Lys  Gly Leu Asn Gly Gly  Lys Phe His Gln Pro  Ile Phe Lys 
    1340                 1345                 1350             


Val Arg  Thr Tyr Glu Pro Lys  Gly Asn Lys Phe Gln  Val Gly Phe 
    1355                 1360                 1365             


Lys Gly  Asn Lys Asn Ser Lys  Tyr Val Glu Ala Ala  Lys Gly Thr 
    1370                 1375                 1380             


Asn Leu  Phe Phe Ala Ile Tyr  Leu Asn Asp Glu Gly  Lys Arg Ser 
    1385                 1390                 1395             


Tyr Glu  Thr Ile Pro Phe Asn  Val Val Met Glu Arg  Leu Lys Gln 
    1400                 1405                 1410             


Gly Glu  Gly Pro Val Pro Gln  Thr Asn Glu Lys Gly  Gly Lys Leu 
    1415                 1420                 1425             


Leu Phe  His Leu Ser Pro Asn  Glu Leu Val Tyr Val  Pro Glu Thr 
    1430                 1435                 1440             


Asp Glu  Asn Tyr Thr Asn Val  Asp Phe Lys Asn Leu  Ser Lys Glu 
    1445                 1450                 1455             


Gln Val  Glu Lys Ile Tyr Lys  Met Val Ser Ser Thr  Gly Ser Glu 
    1460                 1465                 1470             


Cys His  Phe Val Arg Ala Asp  Ile Ser Ser Leu Ile  Lys Gly Tyr 
    1475                 1480                 1485             


Asp Ala  Lys Thr Lys Val Gly  Glu Leu Gly Ser Leu  Asn Lys Gln 
    1490                 1495                 1500             


Glu Val  Thr Asn Asn Gly Met  Thr Arg Ile Lys Glu  Lys Cys Trp 
    1505                 1510                 1515             


Lys Ile  Lys Ile Asp Arg Leu  Gly Ser Ile 
    1520                 1525             


<210>  1332
<211>  1528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1332

Met Lys Lys Ile Leu Gly Ile Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Ser Phe Asp Glu Asn Gln Asn Glu Glu Ser Leu Lys 
            20                  25                  30          


His Ile Glu Cys Val Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile 
        35                  40                  45              


Leu Gly Asp Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ala Lys Arg 
    50                  55                  60                  


Thr Glu Phe Arg Ser Val Arg His Leu Arg Glu Arg His Leu Leu Arg 
65                  70                  75                  80  


Arg Ala Arg Leu His Lys Ile Leu Ser Leu Leu Gly Phe Leu Pro Asp 
                85                  90                  95      


His Tyr Val Gln Asp Leu Asp Ser His Gly Asn Phe Pro Val Asn Val 
            100                 105                 110         


Glu Pro Lys Ile Ala Trp Arg Lys Asp Ser Asn Gly Ala Phe Glu Phe 
        115                 120                 125             


Ile Phe Asn Asp Ser Phe Asp Glu Met Leu Gln Asp Phe Ala Thr Tyr 
    130                 135                 140                 


Gln Pro Glu Leu Ile Ala Cys Asn Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Lys Lys Leu Ser Lys Glu 
                165                 170                 175     


Glu Leu Ser Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Asp Asp Glu Ser Val Lys Ser Ala Lys Thr 
        195                 200                 205             


Arg Gln Tyr Phe Asp Arg Gln Lys Ile Lys Ser Ile Val Asp Thr Asn 
    210                 215                 220                 


Gln Thr Tyr Lys Gly Gln Lys Val Leu Ile Val Glu Leu Glu Asp Gly 
225                 230                 235                 240 


Ile Lys Gly Lys Ile Phe Lys Lys Glu Ile Pro Asn Trp Val Gly Gln 
                245                 250                 255     


Glu Lys Ser Ile Ile Val Thr Ile Asp Ile Asp Lys Asp Gly Cys Asp 
            260                 265                 270         


Lys Tyr Glu Glu Ser Gly Glu Leu Ser Cys Arg Phe Lys Ile Pro Ser 
        275                 280                 285             


Asp Ala Glu Trp Glu Ser Glu Trp Lys Leu Ile Lys Met Lys Thr Gln 
    290                 295                 300                 


Lys Asp Leu Asp Ala Ser Gly Lys Thr Val Gly Ala Tyr Ile Tyr Asp 
305                 310                 315                 320 


Ala Ile Leu Gln Asn Pro Ser Gln Lys Ile Arg Gly Lys Leu Val Arg 
                325                 330                 335     


Thr Ile Glu Arg Lys Tyr Tyr Lys Glu Glu Leu Lys Gln Ile Leu Lys 
            340                 345                 350         


Lys Gln Val Glu Phe His Pro Glu Leu Arg Asp Lys Asp Leu Phe Leu 
        355                 360                 365             


Asp Cys Leu Asn Glu Leu Tyr Pro Asn Asn Asp Ala His Arg Asn Ala 
    370                 375                 380                 


Cys Ala Asp Arg Asp Phe Ile Tyr Leu Phe Met Asp Asp Ile Leu Phe 
385                 390                 395                 400 


Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asp Cys Pro 
                405                 410                 415     


Tyr Glu Lys His Met Tyr Arg Asp Lys Glu Thr Gly Glu Leu Lys Tyr 
            420                 425                 430         


Ser Ser Val Lys Cys Ile Ala Lys Ser His Pro Leu Phe Gln Glu Phe 
        435                 440                 445             


Arg Leu Trp His Phe Ile Ser Asn Ile Arg Ile Tyr Gln Lys Glu Arg 
    450                 455                 460                 


Val Val Asp Val Asp Ala Asp Gln Ser Ala Leu Ser Ser Ala Arg Ser 
465                 470                 475                 480 


Ala Leu Lys Ser Lys Leu Glu Ala Asp Val Asp Val Thr Asn Glu Phe 
                485                 490                 495     


Leu Thr Cys Glu Ala Asp Tyr Val Ser Leu Phe Glu Trp Leu Asn Asn 
            500                 505                 510         


Lys Lys Ser Val Ser Gln Lys Asp Phe Leu Lys Tyr Pro Ala Phe Gly 
        515                 520                 525             


Ile Lys Lys Ser Gly Asp Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys 
    530                 535                 540                 


Ser Tyr Pro Cys Asn Glu Thr Arg Ala Leu Ile Leu Ser His Leu Glu 
545                 550                 555                 560 


Lys Ala Asn Ile Arg Gly Asp Ile Phe Thr Arg Glu Gln Glu Glu Ala 
                565                 570                 575     


Leu Trp His Ile Leu Tyr Ser Val Glu Asp Arg Tyr Glu Ile Val Lys 
            580                 585                 590         


Ala Leu Gln Ser Phe Ala Ala Lys Tyr Arg Phe Asn Asp Asp Phe Val 
        595                 600                 605             


Thr Val Phe Ser Lys Phe Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr 
    610                 615                 620                 


Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Met Gly Lys 
625                 630                 635                 640 


Tyr Trp Ser Glu Ala Ala Ile Asp Ile Lys Thr Arg Glu Arg Ile Glu 
                645                 650                 655     


Lys Ile Ile Thr Gly Glu Cys Asp Asp Ser Ile Arg Glu Arg Val Arg 
            660                 665                 670         


Leu Lys Ala Ile Arg Leu His Asp Ile Gly Asp Phe Arg Ala Leu Pro 
        675                 680                 685             


Val Trp Leu Ala Cys Tyr Val Val Tyr Asp Arg His Ser Glu Ser Gly 
    690                 695                 700                 


Asp Ile Thr Lys Trp Lys Thr Pro Gln Glu Met Asp Trp Tyr Ile Gln 
705                 710                 715                 720 


Asn Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile 
                725                 730                 735     


Thr Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Gln Val Gly Arg 
            740                 745                 750         


Ile Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys Asn Pro Ala 
        755                 760                 765             


Asp Lys Arg Ala Lys Met Thr Ala Arg Met Ile Glu Asn Glu Asn Thr 
    770                 775                 780                 


Asn Leu Arg Ile Lys Ala Met Leu Thr Glu Phe Leu Asn Pro Glu Phe 
785                 790                 795                 800 


Glu Ile Glu Asn Val Arg Pro Tyr Ser Ala Ser Gln Gln Asp Ile Leu 
                805                 810                 815     


Arg Ile Tyr Glu Asn Gly Val Leu Asn Ser Val Ser Glu Ile Pro Glu 
            820                 825                 830         


Glu Ile Thr Gly Ile Leu Lys Lys Phe Asn Glu Arg Glu Gln Arg Lys 
        835                 840                 845             


Arg Pro Ser Thr Ser Glu Val Leu Arg Tyr Lys Leu Trp Leu Glu Gln 
    850                 855                 860                 


Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Met Ile Pro Leu Gly Lys Leu 
865                 870                 875                 880 


Phe Thr Pro Ala Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr 
                885                 890                 895     


Phe Asp Asp Ser Phe Asn Asn Lys Val Ile Cys Glu Ala Glu Val Asn 
            900                 905                 910         


Lys Leu Lys Gly Asn Leu Leu Gly Tyr Glu Phe Ile Lys Lys His His 
        915                 920                 925             


Gly Arg Arg Val Asp Leu Gly Leu Gly Glu Ile Val Asp Ile Phe Ser 
    930                 935                 940                 


Val Asp Glu Tyr Glu Thr Phe Val Lys Asp Tyr Tyr Ser Ala Asn Gln 
945                 950                 955                 960 


Gln Lys Met Lys Lys Leu Leu Met Asp Glu Ile Pro Glu Gln Phe Ile 
                965                 970                 975     


Glu Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser 
            980                 985                 990         


Leu Leu Ser Asn Ile Val Arg Glu  Gln Asp Glu Gln Glu  Ser Ile Ser 
        995                 1000                 1005             


Lys Asn  Leu Ile Val Cys Thr  Gly Gly Val Thr Asp  Arg Leu Lys 
    1010                 1015                 1020             


Lys Asp  Trp Gly Ile Asn Asp  Val Trp Asn Lys Leu  Ile Leu Pro 
    1025                 1030                 1035             


Arg Phe  Gln Arg Leu Asn Asn  Ile Thr Glu Thr Thr  Glu Phe Thr 
    1040                 1045                 1050             


Ala Lys  Asn Met Gln Gly His  Glu Ile Pro Val Val  Pro Phe Glu 
    1055                 1060                 1065             


Tyr Gln  Arg Gly Phe Asn Lys  Lys Arg Ile Asp His  Arg His His 
    1070                 1075                 1080             


Ala Met  Asp Ala Ile Val Ile  Ala Cys Ala Gly Arg  Asn Ile Val 
    1085                 1090                 1095             


Asn Tyr  Leu Asn Asn Glu Ser  Ala Arg Lys Lys Ala  Gln Ile Ser 
    1100                 1105                 1110             


Arg Tyr  Asp Leu Gln Arg Leu  Leu Cys Asp Lys Val  Lys Thr Asp 
    1115                 1120                 1125             


Asp Asn  Gly Asn Tyr Lys Trp  Ile Leu Lys Lys Pro  Trp Ser Thr 
    1130                 1135                 1140             


Leu Thr  Gln Asp Val Tyr Ala  Ala Leu Asp Asn Val  Ile Val Ser 
    1145                 1150                 1155             


Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys Thr Asn  Asn His Tyr 
    1160                 1165                 1170             


Val His  Tyr Glu Ala Gly Lys  Lys Ile Ile Ala Lys  Gln Glu Lys 
    1175                 1180                 1185             


Ala Ser  Trp Ala Ile Arg Lys  Pro Met His Lys Asp  Thr Val Phe 
    1190                 1195                 1200             


Gly Glu  Val Asn Leu Arg Lys  Val Lys Thr Val Ser  Leu Asn Glu 
    1205                 1210                 1215             


Ala Ile  Lys Ser Pro His Cys  Ile Val Gln Lys Asp  Phe Lys Lys 
    1220                 1225                 1230             


Lys Leu  Leu Ser Leu Leu Lys  Gln Asn Phe Asp Ala  Lys Glu Val 
    1235                 1240                 1245             


Lys Ala  Tyr Phe Glu Glu His  Lys Asp Val Trp Ser  Asp Ile Asn 
    1250                 1255                 1260             


Leu Ser  Lys Ile Glu Val Tyr  Tyr Phe Thr Lys Asp  Thr Lys Glu 
    1265                 1270                 1275             


Arg Phe  Phe Ala Thr Arg Lys  Pro Leu Asp Ser Ser  Phe Asp Arg 
    1280                 1285                 1290             


Lys Lys  Ile Glu Ser Val Thr  Asp Thr Gly Ile Gln  Lys Ile Leu 
    1295                 1300                 1305             


Leu Arg  His Leu Glu Gln Lys  Asn Gly Asp Ser Asp  Leu Ala Phe 
    1310                 1315                 1320             


Ser Pro  Asp Gly Ile Glu Glu  Met Asn Gln Asn Ile  Val Gln Leu 
    1325                 1330                 1335             


Asn Gly  Gly Arg Lys His Gln  Pro Ile Val Lys Val  Arg Val Tyr 
    1340                 1345                 1350             


Glu Lys  Ala Asp Lys Phe Ala  Ile Gly Gln Gln Gly  Asn Lys Ala 
    1355                 1360                 1365             


Ser Lys  Tyr Val Glu Ala Ala  Lys Gly Thr Asn Leu  Phe Phe Ala 
    1370                 1375                 1380             


Val Tyr  Glu Glu Glu Val Arg  Asp Lys Lys Thr Asn  Ala Val Met 
    1385                 1390                 1395             


Phe Lys  Arg Asn Tyr Ala Ser  Ile Pro Leu Asn Val  Ala Ile Asp 
    1400                 1405                 1410             


Arg Gln  Lys Lys Gly Leu Ser  Thr Ala Pro Glu Asp  Glu Asn Gly 
    1415                 1420                 1425             


Asn Ala  Pro Ile Phe Val Leu  Ser Pro Asn Asp Leu  Val Tyr Leu 
    1430                 1435                 1440             


Pro Thr  Ala Glu Asp Ile Ile  Asn Ala Arg Ile Ser  Phe Pro Ile 
    1445                 1450                 1455             


Asn Lys  Asn Arg Ile Tyr Lys  Met Val Ser Cys Thr  Gly Tyr Gln 
    1460                 1465                 1470             


Cys Phe  Phe Ile Lys His His  Val Ala Asn Val Ile  Val Asp Lys 
    1475                 1480                 1485             


Lys Glu  Phe Ser Ala Leu Asn  Lys Met Glu Lys Ala  Glu Thr Asn 
    1490                 1495                 1500             


Glu Met  Ile Lys Asp Ile Cys  Val Pro Ile Lys Leu  Asp Arg Leu 
    1505                 1510                 1515             


Gly Asn  Ile Val Lys Ile Gly  Val Tyr Lys 
    1520                 1525             


<210>  1333
<211>  1528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1333

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Ser Phe Asp Glu Asn Gln Asn Glu Glu Ser Leu Lys 
            20                  25                  30          


His Ile Glu Cys Val Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile 
        35                  40                  45              


Leu Gly Asp Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ala Lys Arg 
    50                  55                  60                  


Thr Glu Phe Arg Ser Val Arg His Leu Arg Glu Arg His Leu Leu Arg 
65                  70                  75                  80  


Arg Ala Arg Leu His Lys Ile Leu Ser Leu Leu Gly Phe Leu Pro Asp 
                85                  90                  95      


His Tyr Val Gln Asp Leu Asp Ser His Gly Asn Phe Pro Val Asn Val 
            100                 105                 110         


Glu Pro Lys Ile Ala Trp Arg Lys Asp Ser Asn Gly Ala Phe Glu Phe 
        115                 120                 125             


Ile Phe Asn Asp Ser Phe Asp Glu Met Leu Gln Asp Phe Ala Thr Tyr 
    130                 135                 140                 


Gln Pro Glu Leu Ile Ala Cys Asn Lys Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Lys Lys Leu Ser Lys Glu 
                165                 170                 175     


Glu Leu Ser Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Asp Asp Glu Ser Val Lys Ser Ala Lys Thr 
        195                 200                 205             


Arg Gln Tyr Phe Asp Arg Gln Lys Ile Lys Ser Ile Val Asp Thr Asn 
    210                 215                 220                 


Gln Thr Tyr Lys Gly Gln Lys Val Leu Ile Val Glu Leu Glu Asp Gly 
225                 230                 235                 240 


Ile Lys Gly Lys Ile Phe Lys Lys Glu Ile Pro Asn Trp Val Gly Gln 
                245                 250                 255     


Glu Lys Ser Ile Ile Val Thr Ile Asp Ile Asp Lys Asp Gly Cys Asp 
            260                 265                 270         


Lys Tyr Glu Glu Ser Gly Glu Leu Ser Cys Arg Phe Lys Ile Pro Ser 
        275                 280                 285             


Asp Ala Glu Trp Glu Ser Glu Trp Lys Leu Ile Lys Met Lys Thr Gln 
    290                 295                 300                 


Lys Asp Leu Asp Ala Ser Gly Lys Thr Val Gly Ala Tyr Ile Tyr Asp 
305                 310                 315                 320 


Ala Ile Leu Gln Asn Pro Ser Gln Lys Ile Arg Gly Lys Leu Val Arg 
                325                 330                 335     


Thr Ile Glu Arg Lys Tyr Tyr Lys Glu Glu Leu Lys Gln Ile Leu Lys 
            340                 345                 350         


Lys Gln Val Glu Phe His Pro Glu Leu Arg Asp Lys Asp Leu Phe Leu 
        355                 360                 365             


Asp Cys Leu Asn Glu Leu Tyr Pro Asn Asn Asp Ala His Arg Asn Ala 
    370                 375                 380                 


Cys Ala Asp Arg Asp Phe Ile Tyr Leu Phe Met Asp Asp Ile Leu Phe 
385                 390                 395                 400 


Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asp Cys Pro 
                405                 410                 415     


Tyr Glu Lys His Met Tyr Arg Asp Lys Glu Thr Gly Glu Leu Lys Tyr 
            420                 425                 430         


Ser Ser Val Lys Cys Ile Ala Lys Ser His Pro Leu Phe Gln Glu Phe 
        435                 440                 445             


Arg Leu Trp His Phe Ile Ser Asn Ile Arg Ile Tyr Gln Lys Glu Arg 
    450                 455                 460                 


Val Val Asp Val Asp Ala Cys Gln Pro Asp Leu Ser Ser Ala Arg Ser 
465                 470                 475                 480 


Ala Leu Lys Ser Lys Leu Glu Ala Asp Val Asp Val Thr Asn Glu Phe 
                485                 490                 495     


Leu Thr Cys Glu Ala Asp Tyr Val Ser Leu Phe Glu Trp Leu Asn Asn 
            500                 505                 510         


Lys Lys Ser Val Ser Gln Lys Asp Phe Leu Lys Tyr Pro Ala Phe Gly 
        515                 520                 525             


Ile Lys Lys Ser Gly Asp Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys 
    530                 535                 540                 


Ser Tyr Pro Cys Asn Glu Thr Arg Ala Leu Ile Leu Ser His Leu Glu 
545                 550                 555                 560 


Lys Ala Asn Ile Arg Gly Asp Ile Phe Thr Arg Glu Gln Glu Glu Ala 
                565                 570                 575     


Leu Trp His Ile Leu Tyr Ser Val Glu Asp Arg Tyr Glu Ile Val Lys 
            580                 585                 590         


Ala Leu Gln Ser Phe Ala Ala Lys Tyr Arg Phe Asn Asp Asp Phe Val 
        595                 600                 605             


Thr Val Phe Ser Lys Phe Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr 
    610                 615                 620                 


Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Met Gly Lys 
625                 630                 635                 640 


Tyr Trp Ser Glu Ala Ala Ile Asp Ile Lys Thr Arg Glu Arg Ile Glu 
                645                 650                 655     


Lys Ile Ile Thr Gly Glu Cys Asp Asp Ser Ile Arg Glu Arg Val Arg 
            660                 665                 670         


Leu Lys Ala Ile Arg Leu His Asp Ile Gly Asp Phe Arg Ala Leu Pro 
        675                 680                 685             


Val Trp Leu Ala Cys Tyr Val Val Tyr Asp Arg His Ser Glu Ser Gly 
    690                 695                 700                 


Asp Ile Thr Lys Trp Lys Thr Pro Gln Glu Met Asp Trp Tyr Ile Gln 
705                 710                 715                 720 


Asn Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile 
                725                 730                 735     


Thr Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Gln Val Gly Arg 
            740                 745                 750         


Ile Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys Asn Pro Ala 
        755                 760                 765             


Asp Lys Arg Ala Lys Met Thr Ala Arg Met Ile Glu Asn Glu Asn Thr 
    770                 775                 780                 


Asn Leu Arg Ile Lys Ala Met Leu Thr Glu Phe Leu Asn Pro Glu Phe 
785                 790                 795                 800 


Lys Ile Glu Asn Val Arg Pro Tyr Ser Ala Ser Gln Gln Asp Ile Leu 
                805                 810                 815     


Arg Ile Tyr Glu Asn Gly Val Leu Asn Ser Val Ser Glu Ile Pro Glu 
            820                 825                 830         


Glu Ile Thr Gly Ile Leu Lys Lys Phe Asn Glu Arg Glu Gln Arg Lys 
        835                 840                 845             


Arg Pro Ser Thr Ser Glu Val Leu Arg Tyr Lys Leu Trp Leu Glu Gln 
    850                 855                 860                 


Lys Tyr Arg Ser Pro Tyr Thr Gly Lys Met Ile Pro Leu Gly Lys Leu 
865                 870                 875                 880 


Phe Thr Pro Ala Tyr Glu Ile Glu His Ile Ile Pro Gln Ser Arg Tyr 
                885                 890                 895     


Phe Asp Asp Ser Phe Asn Asn Lys Val Ile Cys Glu Ala Glu Val Asn 
            900                 905                 910         


Lys Leu Lys Gly Asn Leu Leu Gly Tyr Glu Phe Ile Lys Lys His His 
        915                 920                 925             


Gly Arg Arg Val Asp Leu Gly Leu Gly Glu Ile Val Asp Ile Phe Ser 
    930                 935                 940                 


Val Asp Glu Tyr Glu Thr Phe Val Lys Asp Tyr Tyr Ser Ala Asn Gln 
945                 950                 955                 960 


Gln Lys Met Lys Lys Leu Leu Met Asp Glu Ile Pro Glu Gln Phe Ile 
                965                 970                 975     


Glu Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser 
            980                 985                 990         


Leu Leu Ser Asn Ile Val Arg Glu  Gln Asp Glu Gln Glu  Ser Ile Ser 
        995                 1000                 1005             


Lys Asn  Leu Ile Val Cys Thr  Gly Gly Val Thr Asp  Arg Leu Lys 
    1010                 1015                 1020             


Lys Asp  Trp Gly Ile Asn Asp  Val Trp Asn Lys Leu  Ile Leu Pro 
    1025                 1030                 1035             


Arg Phe  Gln Arg Leu Asn Asn  Ile Thr Glu Thr Thr  Glu Phe Thr 
    1040                 1045                 1050             


Ala Lys  Asn Met Gln Gly His  Glu Ile Pro Val Val  Pro Phe Glu 
    1055                 1060                 1065             


Tyr Gln  Arg Gly Phe Asn Lys  Lys Arg Ile Asp His  Arg His His 
    1070                 1075                 1080             


Ala Met  Asp Ala Ile Val Ile  Ala Cys Ala Gly Arg  Asn Ile Val 
    1085                 1090                 1095             


Asn Tyr  Leu Asn Asn Glu Ser  Ala Arg Lys Lys Ala  Gln Ile Ser 
    1100                 1105                 1110             


Arg Tyr  Asp Leu Gln Arg Leu  Leu Cys Asp Lys Val  Lys Thr Asp 
    1115                 1120                 1125             


Asp Asn  Gly Asn Tyr Lys Trp  Ile Leu Lys Lys Pro  Trp Ser Thr 
    1130                 1135                 1140             


Leu Thr  Gln Asp Val Tyr Ala  Ala Leu Asp Asn Val  Ile Val Ser 
    1145                 1150                 1155             


Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys Thr Asn  Asn His Tyr 
    1160                 1165                 1170             


Val His  Tyr Glu Ala Gly Lys  Lys Ile Ile Ala Lys  Gln Glu Lys 
    1175                 1180                 1185             


Ala Ser  Trp Ala Ile Arg Lys  Pro Met His Lys Asp  Thr Val Phe 
    1190                 1195                 1200             


Gly Glu  Val Asn Leu Arg Lys  Val Lys Thr Val Ser  Leu Asn Glu 
    1205                 1210                 1215             


Ala Ile  Lys Ser Pro His Cys  Ile Val Gln Lys Asp  Phe Lys Lys 
    1220                 1225                 1230             


Lys Leu  Leu Ser Leu Leu Lys  Gln Asn Phe Asp Ala  Lys Glu Val 
    1235                 1240                 1245             


Lys Ala  Tyr Phe Glu Glu His  Lys Asp Val Trp Ser  Asp Ile Asn 
    1250                 1255                 1260             


Leu Ser  Lys Ile Glu Val Tyr  Tyr Phe Thr Lys Asp  Thr Lys Glu 
    1265                 1270                 1275             


Arg Phe  Phe Ala Thr Arg Lys  Pro Leu Asp Ser Ser  Phe Asp Arg 
    1280                 1285                 1290             


Lys Lys  Ile Glu Ser Val Thr  Asp Thr Gly Ile Gln  Lys Ile Leu 
    1295                 1300                 1305             


Leu Arg  His Leu Glu Gln Lys  Asn Gly Asp Ser Asp  Leu Ala Phe 
    1310                 1315                 1320             


Ser Pro  Asp Gly Ile Glu Glu  Met Asn Gln Asn Ile  Val Gln Leu 
    1325                 1330                 1335             


Asn Gly  Gly Arg Lys His Gln  Pro Ile Val Lys Val  Arg Val Tyr 
    1340                 1345                 1350             


Glu Lys  Ala Asp Lys Phe Ala  Ile Gly Gln Gln Gly  Asn Lys Ala 
    1355                 1360                 1365             


Ser Lys  Tyr Val Glu Ala Ala  Lys Gly Thr Asn Leu  Phe Phe Ala 
    1370                 1375                 1380             


Val Tyr  Glu Glu Glu Val Arg  Asp Lys Lys Thr Asn  Ala Val Met 
    1385                 1390                 1395             


Phe Lys  Arg Asn Tyr Ala Ser  Ile Pro Leu Asn Val  Ala Ile Asp 
    1400                 1405                 1410             


Arg Gln  Lys Lys Gly Leu Ser  Thr Ala Pro Glu Asp  Glu Asn Gly 
    1415                 1420                 1425             


Asn Ala  Pro Ile Phe Val Leu  Ser Pro Asn Asp Leu  Val Tyr Leu 
    1430                 1435                 1440             


Pro Thr  Ala Glu Asp Ile Ile  Asn Ala Arg Ile Ser  Phe Pro Ile 
    1445                 1450                 1455             


Asn Lys  Asn Arg Ile Tyr Lys  Met Val Ser Cys Thr  Gly Tyr Gln 
    1460                 1465                 1470             


Cys Phe  Phe Ile Lys His His  Val Ala Asn Val Ile  Val Asp Lys 
    1475                 1480                 1485             


Lys Glu  Phe Ser Ala Leu Asn  Lys Met Glu Lys Ala  Glu Thr Asn 
    1490                 1495                 1500             


Glu Met  Ile Lys Asp Ile Cys  Val Pro Ile Lys Leu  Asp Arg Leu 
    1505                 1510                 1515             


Gly Asn  Ile Val Lys Ile Gly  Val Tyr Lys 
    1520                 1525             


<210>  1334
<211>  1529
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1334

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Val Val Asn Ser Phe Asp Glu Asn Gln Asn Glu Glu Ser Leu Lys 
            20                  25                  30          


His Ile Glu Cys Leu Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Ile 
        35                  40                  45              


Leu Gly Asp Phe Asp Lys Gly Asn Ser Ile Ser Gln Thr Ala Lys Arg 
    50                  55                  60                  


Thr Glu Phe Arg Gly Val Arg His Leu Arg Glu Arg His Leu Leu Arg 
65                  70                  75                  80  


Arg Ala Arg Leu His Lys Ile Leu Arg Leu Leu Gly Phe Leu Pro Asp 
                85                  90                  95      


His Tyr Val Gln Asp Leu Asp Gly His Gly Asn Phe Leu Val Asn Val 
            100                 105                 110         


Glu Pro Lys Ile Ala Trp Arg Lys Asp Ser Asn Gly Thr Phe Glu Phe 
        115                 120                 125             


Ile Phe Asn Asp Ser Phe Asp Glu Met Leu Gln Asp Phe Ala Thr Tyr 
    130                 135                 140                 


Gln Pro Glu Leu Ile Ala Cys Asn Arg Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Lys Lys Leu Ser Lys Glu 
                165                 170                 175     


Glu Leu Ser Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Glu Asp Asp Glu Ser Val Lys Thr Ala Lys Thr 
        195                 200                 205             


Arg Gln Tyr Phe Asp Arg Gln Lys Ile Lys Ser Ile Val Asp Thr Asn 
    210                 215                 220                 


Gln Thr Tyr Lys Gly Gln Lys Val Leu Ile Val Glu Leu Glu Asp Gly 
225                 230                 235                 240 


Val Lys Gly Lys Ile Phe Lys Lys Glu Ile Pro Asn Trp Val Gly Gln 
                245                 250                 255     


Glu Lys Ser Ile Ile Val Thr Val Asp Ile Asp Lys Asp Gly Cys Asp 
            260                 265                 270         


Lys Tyr Glu Glu Ser Gly Glu Leu Ser Cys Arg Phe Lys Ile Pro Ser 
        275                 280                 285             


Asp Ala Glu Trp Glu Ser Glu Trp Lys Leu Ile Lys Met Lys Thr Gln 
    290                 295                 300                 


Lys Asp Leu Asp Ala Ser Gly Lys Thr Val Gly Ala Tyr Ile Tyr Asp 
305                 310                 315                 320 


Ala Ile Leu Gln Asn Pro Ser Gln Lys Ile Arg Gly Lys Leu Val Arg 
                325                 330                 335     


Thr Ile Glu Arg Lys Tyr Tyr Lys Glu Glu Leu Lys Gln Ile Leu Lys 
            340                 345                 350         


Lys Gln Val Glu Phe His Pro Glu Leu Arg Asp Lys Asp Leu Phe Leu 
        355                 360                 365             


Asp Cys Leu Asn Glu Leu Tyr Pro Asn Asn Asp Ala His Arg Asn Ala 
    370                 375                 380                 


Cys Ala Asp Arg Asp Phe Ile Tyr Leu Phe Met Asp Asp Ile Leu Phe 
385                 390                 395                 400 


Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ala Asp Cys Pro 
                405                 410                 415     


Tyr Glu Lys His Met Tyr Arg Asp Lys Glu Thr Gly Glu Leu Lys Tyr 
            420                 425                 430         


Ser Ser Val Lys Cys Ile Ala Lys Ser His Pro Leu Phe Gln Glu Phe 
        435                 440                 445             


Arg Leu Trp His Phe Ile Ser Asn Ile Arg Ile Tyr Gln Lys Glu Arg 
    450                 455                 460                 


Val Ile Asp Val Asp Ala Cys Gln Pro Asp Leu Ser Ser Ala Arg Ser 
465                 470                 475                 480 


Ala Leu Lys Ser Lys Leu Glu Ala Asp Val Asp Val Thr Asn Glu Phe 
                485                 490                 495     


Leu Thr Cys Glu Ala Asp Tyr Val Ser Leu Phe Glu Trp Leu Asn Asn 
            500                 505                 510         


Lys Lys Ser Val Ser Gln Lys Asp Phe Leu Lys Tyr Pro Ala Phe Gly 
        515                 520                 525             


Ile Lys Lys Ser Gly Asp Asn Tyr Arg Trp Asn Tyr Val Glu Asp Lys 
    530                 535                 540                 


Ser Tyr Pro Cys Asn Glu Thr Arg Ala Leu Ile Leu Ser His Leu Glu 
545                 550                 555                 560 


Lys Ala Asn Ile Arg Gly Asp Ile Phe Thr Arg Glu Gln Glu Glu Ala 
                565                 570                 575     


Leu Trp His Ile Leu Tyr Ser Val Glu Asp Arg Tyr Glu Ile Val Lys 
            580                 585                 590         


Ala Leu Gln Ser Phe Ala Ala Lys Tyr Arg Phe Asn Asp Asp Phe Val 
        595                 600                 605             


Thr Val Phe Ser Lys Phe Pro Pro Phe Lys Lys Glu Tyr Gly Ser Tyr 
    610                 615                 620                 


Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Met Gly Lys 
625                 630                 635                 640 


Tyr Trp Ser Glu Ser Ala Ile Asp Ile Lys Thr Arg Glu Arg Ile Glu 
                645                 650                 655     


Lys Ile Ile Thr Gly Glu Cys Asp Asp Ser Ile Arg Glu Arg Val Arg 
            660                 665                 670         


Leu Lys Ala Ile Arg Leu His Asp Ile Glu His Phe Arg Ala Leu Pro 
        675                 680                 685             


Val Trp Leu Ala Cys Tyr Val Val Tyr Asp Arg His Ser Glu Ser Gly 
    690                 695                 700                 


Asp Ile Met Lys Trp Lys Thr Pro Gln Glu Met Asp Ser Tyr Ile Gln 
705                 710                 715                 720 


Asn Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile 
                725                 730                 735     


Thr Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Gln Val Gly Arg 
            740                 745                 750         


Ile Asp Glu Ile His Val Glu Leu Gly Arg Glu Met Lys Asn Pro Ala 
        755                 760                 765             


Asp Lys Arg Lys Glu Met Thr Lys Arg Ile Ile Glu Asn Glu Asn Thr 
    770                 775                 780                 


Asn Leu Arg Ile Lys Ala Met Leu Thr Glu Phe Leu Asn Pro Glu Phe 
785                 790                 795                 800 


Glu Ile Glu Asn Val Arg Pro Tyr Ser Ala Ser Gln Gln Asp Ile Leu 
                805                 810                 815     


Arg Ile Tyr Glu Asn Gly Val Leu Asn Ser Val Ser Glu Ile Pro Glu 
            820                 825                 830         


Glu Ile Thr Gly Ile Leu Lys Lys Phe Asn Glu Arg Glu Gln Arg Lys 
        835                 840                 845             


Arg Pro Ser Thr Ser Glu Val Leu Arg Tyr Lys Leu Trp Leu Glu Gln 
    850                 855                 860                 


Lys Tyr Cys Ser Pro Tyr Thr Gly Lys Met Ile Pro Leu Gly Lys Leu 
865                 870                 875                 880 


Phe Thr Pro Ala Tyr Glu Ile Glu His Val Ile Pro Gln Ser Arg Tyr 
                885                 890                 895     


Phe Asp Asp Ser Phe Asn Asn Lys Val Ile Cys Glu Ala Glu Val Asn 
            900                 905                 910         


Lys Leu Lys Gly Asn Leu Leu Gly Tyr Glu Phe Ile Lys Lys His His 
        915                 920                 925             


Gly Gln Arg Val Asp Leu Gly Leu Gly Glu Ile Val Asp Ile Phe Ser 
    930                 935                 940                 


Val Asp Glu Tyr Glu Thr Phe Val Lys Asp Tyr Tyr Ser Gly Asn Gln 
945                 950                 955                 960 


Gln Lys Met Lys Lys Leu Leu Met Asp Glu Ile Pro Glu Gln Phe Ile 
                965                 970                 975     


Glu Arg Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Val Val Lys Ser 
            980                 985                 990         


Leu Leu Ser Asn Ile Val Arg Glu  Gln Asp Glu Gln Glu  Ser Ile Ser 
        995                 1000                 1005             


Lys Asn  Val Ile Val Cys Thr  Gly Gly Val Thr Asp  Arg Leu Lys 
    1010                 1015                 1020             


Lys Asp  Trp Gly Ile Asn Asp  Val Trp Asn Lys Leu  Ile Leu Pro 
    1025                 1030                 1035             


Arg Phe  Gln Arg Leu Asn Asn  Ile Thr Glu Thr Thr  Glu Phe Thr 
    1040                 1045                 1050             


Ala Lys  Asn Met Glu Gly His  Glu Ile Pro Val Val  Pro Phe Glu 
    1055                 1060                 1065             


Tyr Gln  Arg Gly Phe Asn Lys  Lys Arg Ile Asp His  Arg His His 
    1070                 1075                 1080             


Ala Met  Asp Ala Ile Val Ile  Ala Cys Ala Ser Arg  Asn Ile Val 
    1085                 1090                 1095             


Asn Tyr  Leu Asn Asn Glu Ser  Ala Arg Lys Lys Ala  Arg Ile Ser 
    1100                 1105                 1110             


Arg Tyr  Asp Leu Gln Lys Leu  Leu Cys Asp Lys Val  Arg Thr Asp 
    1115                 1120                 1125             


Asp Asn  Gly Asn Tyr Lys Trp  Ile Leu Lys Lys Pro  Trp Gly Thr 
    1130                 1135                 1140             


Leu Thr  Gln Asp Val Tyr Ala  Ala Leu Asp Asn Val  Ile Val Ser 
    1145                 1150                 1155             


Phe Lys  Gln Asn Leu Arg Val  Ile Asn Lys Thr Lys  Asn Tyr Tyr 
    1160                 1165                 1170             


Val His  Tyr Glu Ala Gly Lys  Lys Thr Ile Ala Lys  Gln Glu Lys 
    1175                 1180                 1185             


Gly Ser  Ser Trp Ala Ile Arg  Lys Pro Met His Lys  Asp Thr Val 
    1190                 1195                 1200             


Phe Gly  Glu Val Asn Leu Arg  Lys Val Lys Thr Val  Ser Leu Asn 
    1205                 1210                 1215             


Glu Ala  Ile Lys Asn Pro Gln  Cys Ile Val Glu Lys  Asp Phe Lys 
    1220                 1225                 1230             


Lys Lys  Leu Leu Ser Leu Leu  Lys Gln Asn Phe Asp  Ala Lys Lys 
    1235                 1240                 1245             


Val Lys  Ala Tyr Phe Glu Glu  His Lys Asp Val Trp  Ser Asp Ile 
    1250                 1255                 1260             


Asn Leu  Ser Lys Ile Glu Val  Tyr Tyr Phe Thr Lys  Asp Thr Lys 
    1265                 1270                 1275             


Glu Arg  Phe Phe Ala Thr Arg  Lys Pro Leu Asp Ser  Ser Phe Asp 
    1280                 1285                 1290             


Lys Lys  Glu Ile Glu Ser Val  Thr Asp Thr Gly Ile  Gln Lys Ile 
    1295                 1300                 1305             


Leu Leu  Arg His Leu Glu Gln  Lys Asn Gly Asp Pro  Asp Leu Ala 
    1310                 1315                 1320             


Phe Ser  Pro Asp Gly Ile Glu  Glu Met Asn His Asn  Ile Val Gln 
    1325                 1330                 1335             


Leu Asn  Gly Gly Arg Lys His  Gln Pro Ile Val Lys  Val Arg Val 
    1340                 1345                 1350             


Tyr Glu  Lys Ala Asp Lys Phe  Ala Ile Gly Gln Gln  Gly Asn Lys 
    1355                 1360                 1365             


Ser Ser  Lys Tyr Val Glu Ala  Ala Lys Gly Thr Asn  Leu Phe Phe 
    1370                 1375                 1380             


Ala Val  Tyr Glu Glu Glu Val  Arg Asp Lys Lys Thr  Asn Ala Val 
    1385                 1390                 1395             


Met Leu  Lys Arg His Tyr Ala  Ser Ile Pro Leu Asn  Val Ala Ile 
    1400                 1405                 1410             


Asp Arg  Gln Lys Lys Gly Leu  Ser Thr Ala Pro Glu  Asp Glu Asn 
    1415                 1420                 1425             


Gly Asn  Val Pro Ile Phe Val  Leu Ser Pro Asn Asp  Leu Val Tyr 
    1430                 1435                 1440             


Leu Pro  Thr Ala Glu Asp Ile  Ala Asn Ala Ser Ile  Ser Phe Pro 
    1445                 1450                 1455             


Ile Asn  Lys Asn Arg Ile Tyr  Lys Met Val Ser Cys  Thr Gly Asn 
    1460                 1465                 1470             


Arg Cys  Phe Phe Ile Lys His  His Val Ala Asn Val  Ile Val Asp 
    1475                 1480                 1485             


Lys Ser  Glu Phe Ser Val Leu  Asn Lys Ile Glu Lys  Ala Glu Thr 
    1490                 1495                 1500             


Asp Glu  Met Ile Lys Glu Ile  Cys Val Pro Ile Lys  Ile Asp Arg 
    1505                 1510                 1515             


Leu Gly  Asn Ile Val Lys Ile  Gly Val Tyr Lys 
    1520                 1525                 


<210>  1335
<211>  1535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1335

Met Asn Thr Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Lys Val Leu Asn Asp Lys Thr Arg Leu Pro Leu Glu Ile 
            20                  25                  30          


Ile Asp Leu Gly Val Val Val Ile Pro Thr Asp Gln Arg Thr Leu Asp 
        35                  40                  45              


Lys Phe Ser Lys Gly Glu Ser Ile Ser Lys Thr Ala Glu Arg Thr Lys 
    50                  55                  60                  


Gln Lys Gly Val Arg Lys Leu Tyr Glu Arg Lys Lys Leu Arg Arg Glu 
65                  70                  75                  80  


Arg Leu His Arg Val Leu Asn Ile Leu Gly Phe Leu Pro Glu His Tyr 
                85                  90                  95      


Ser Lys Lys Ile Asp Phe Lys Lys His Leu Gly Gln Phe Phe Gly Asn 
            100                 105                 110         


Ser Gln Pro Lys Ile Ser Tyr Val Glu Asn Asp His Gly Lys His Glu 
        115                 120                 125             


Phe Ile Phe Met Asp Ser Tyr Arg Glu Met Leu Lys Glu Phe Ser Lys 
    130                 135                 140                 


His Asn Ile Lys Asn Val Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg 
145                 150                 155                 160 


Lys Lys Ala Leu Ser Gln Lys Ile Ser Lys Glu Glu Leu Ala Trp Val 
                165                 170                 175     


Ile Leu Asn Phe Asn Lys Lys Arg Gly Tyr Tyr Gln Phe Arg Asp Asp 
            180                 185                 190         


Asn Ala Asp Glu Asn Lys Glu Asn Lys Ile Glu Lys Tyr Tyr Ala Leu 
        195                 200                 205             


Lys Val Thr Asn Val Lys Lys Glu Asp Ala Gln Asn Tyr Thr Ile Thr 
    210                 215                 220                 


Leu Glu Asn Gly Trp Ile Tyr Lys Asn Lys Ser Lys Ile Ser Leu Leu 
225                 230                 235                 240 


Asp Trp Val Gly Ser Thr Lys Glu Phe Ile Ser Thr Glu Lys Phe Asp 
                245                 250                 255     


Asn Lys Gly Asn Ile Ile Lys Lys Lys Asn Gly Glu Asp Asp Arg Thr 
            260                 265                 270         


Leu Lys Leu Val Asp Ser Glu Lys Asp Trp Ile Ala Ile Lys Lys Ser 
        275                 280                 285             


Thr Glu Asp Arg Ile Glu Asn Tyr Gly Lys Thr Val Gly Thr Tyr Leu 
    290                 295                 300                 


Tyr Glu His Leu Leu Lys Asp Pro Asn Gln Lys Leu Asn Gly Glu Leu 
305                 310                 315                 320 


Ile Asn Ile Ile Asp Arg Lys Phe Tyr Lys Asn Glu Leu Lys Gln Ile 
                325                 330                 335     


Leu Glu Lys Gln Lys Glu Phe His Pro Glu Leu Leu Asn Glu Asp Leu 
            340                 345                 350         


Phe Gln Lys Ser Ile Ile Glu Leu Tyr Pro Lys Asn Ser Thr Arg Gln 
        355                 360                 365             


Lys Glu Leu Lys Asn Lys Asp Leu Cys Tyr Leu Leu Ile Asp Asp Val 
    370                 375                 380                 


Ile Tyr Tyr Gln Arg Asp Leu Lys Lys Lys Asn Ser Thr Ile Asn Lys 
385                 390                 395                 400 


Cys Ser Phe Glu Ser Arg Glu Tyr Tyr Leu Asn Gly Glu Lys Gln Ile 
                405                 410                 415     


Gln Lys Ile Asn Cys Ile Ala Lys Ser Lys Pro Leu Tyr Gln Glu Phe 
            420                 425                 430         


Arg Thr Leu Gln Phe Ile Lys Asn Leu Lys Val Tyr Glu Ile Ile Asp 
        435                 440                 445             


Gly Leu Glu Lys Asp Ile Thr Ser Lys Leu Leu Pro Asp Phe Glu Thr 
    450                 455                 460                 


Lys Glu His Phe Phe Glu Trp Leu Asn Asp Lys Glu Lys Ile Asp Gln 
465                 470                 475                 480 


Leu Gln Leu Leu Ser Tyr Lys Cys Phe Asp Leu His Asp Asn Val Lys 
                485                 490                 495     


Asn Asp Leu Ile Ala Lys Gly Ile Lys Arg Pro Thr Glu Lys Ala Ile 
            500                 505                 510         


Arg Thr Glu Leu Leu Glu Lys Tyr Arg Trp Asn Tyr Ser Lys Asp Asn 
        515                 520                 525             


Asp Arg Asn Phe Leu Leu Asn Glu Thr Arg Phe Lys Ile Leu Lys Tyr 
    530                 535                 540                 


Leu Lys Lys Val Asp Lys Lys Leu Thr Leu Thr Asn Glu Gln Ile Glu 
545                 550                 555                 560 


Ser Leu Trp Leu Leu Leu Tyr Ser Ala Lys Asp Glu Arg Glu Leu Lys 
                565                 570                 575     


Asn Gly Leu Gln Lys Phe Ser Glu Lys Asn Lys Leu Pro Lys Lys Phe 
            580                 585                 590         


Ser Asp Ile Phe Ile Ser Phe Pro Pro Phe Lys Lys Glu Tyr Gly Ala 
        595                 600                 605             


Tyr Ser Glu Lys Ala Leu Lys Lys Leu Leu Ser Leu Val Arg Phe Gly 
    610                 615                 620                 


Lys Tyr Trp Ser Phe Asn Lys Ile Asp Asn Asn Ile Gln Asn Lys Ile 
625                 630                 635                 640 


Met Asn Ile Leu Asn Gly Glu Val Asp Asp Ser Ile Ser Asp Arg Val 
                645                 650                 655     


Arg Glu Lys Thr Ile Leu Leu Gln Asp Lys Asn Asp Phe Gln Asp Leu 
            660                 665                 670         


Pro Leu Trp Leu Ala Ser Tyr Val Ala Tyr Asn Arg Phe Ser Glu Val 
        675                 680                 685             


Ser Glu Ile Lys Tyr Trp Asp Ser Pro Asp Lys Ile Glu Phe Leu Lys 
    690                 695                 700                 


Gln His Ser Ile Gly Asn Pro Ile Val Glu Lys Ile Ile Asn Glu Thr 
705                 710                 715                 720 


Leu Lys Leu Ile Lys Asp Ile Trp Val Ile His Gly Lys Ser Cys Lys 
                725                 730                 735     


Asn Phe Phe Ser Glu Ile His Ile Glu Leu Ser Arg Glu Ile Lys Ser 
            740                 745                 750         


Ser Ser Lys Lys Arg Ala Gln Ile Thr Asn Asn Asn Thr Lys Asn Gln 
        755                 760                 765             


Lys Leu Asn Asn Leu Ile Lys Ser Leu Leu Ile Glu Met Ala Ser Asp 
    770                 775                 780                 


Thr Lys Tyr Glu Asn Val Thr Pro Tyr Ser Pro Ser Gln Gln Glu Ile 
785                 790                 795                 800 


Leu Lys Ile Tyr Asp Glu Gly Ile Tyr Ser Lys Glu Glu Asn Ser Glu 
                805                 810                 815     


Lys Ile Lys Glu Ile Asn Lys Ile Arg Asn Asn Asn Lys Ile Ser Lys 
            820                 825                 830         


Ser Asp Ile Thr Lys Tyr Lys Leu Trp Leu Glu Gln Gly Tyr Ile Ser 
        835                 840                 845             


Pro Tyr Thr Gly Lys Ile Ile Pro Leu Thr Lys Leu Phe Thr Ala Lys 
    850                 855                 860                 


Tyr Gln Lys Glu His Ile Ile Pro Gln Thr Lys Phe Phe Asp Asp Ser 
865                 870                 875                 880 


Phe Asn Asn Lys Val Ile Cys Glu Ala Glu Val Asn Lys Leu Lys Asp 
                885                 890                 895     


Asn Ser Leu Ala Met Asn Phe Ile Lys Ala Lys Gly Gly Lys Ala Val 
            900                 905                 910         


Lys Leu Ser Asp Gly Glu Thr Val Thr Ile Leu Asn Gln Glu Glu Tyr 
        915                 920                 925             


Lys Gln Leu Val Arg Thr Ser Phe Lys Lys Asn Ser Ile Lys Leu Arg 
    930                 935                 940                 


Asn Leu Leu Leu Glu Glu Ile Pro Asp Thr Phe Thr Lys Arg Gln Leu 
945                 950                 955                 960 


Asn Asp Thr Lys Tyr Ile Ser Lys Leu Val Leu Ser Leu Leu Ser Asn 
                965                 970                 975     


Ile Val Arg Glu Lys Gly Glu Leu Glu Ser Thr Ala Lys Arg Ile Val 
            980                 985                 990         


Ser Thr Asn Gly Gln Ile Thr Ser  Ile Leu Arg Asn Asp  Trp Gly Leu 
        995                 1000                 1005             


Asn Asn  Ile Trp Asn Lys Leu  Ile Thr Pro Arg Phe  Glu Arg Leu 
    1010                 1015                 1020             


Asn Asn  Ile Asn Lys Asn Lys  Ile Tyr Gly Asn Trp  Glu Asn Lys 
    1025                 1030                 1035             


Lys Gly  Lys Asn Val Phe Gln  Ile Asn Tyr Leu Asn  Asp Asn Leu 
    1040                 1045                 1050             


Ser Thr  Leu Asn Thr Lys Arg  Ile Asp His Arg His  His Ala Leu 
    1055                 1060                 1065             


Asp Ala  Leu Ile Val Ala Cys  Thr Thr Arg Asn His  Ile Asn Phe 
    1070                 1075                 1080             


Leu Asn  Asn Gln Tyr Ala Lys  Ser Arg Asn Gln Arg  His Asp Leu 
    1085                 1090                 1095             


Arg Ala  Lys Leu Lys Arg Arg  Glu Thr Ile Thr Thr  Glu Gly Ser 
    1100                 1105                 1110             


Lys Lys  Gln Val Phe Lys Asp  Phe Ile Lys Pro Trp  Glu Ser Phe 
    1115                 1120                 1125             


Thr Gln  Asp Ala Leu Lys Lys  Leu Glu Thr Thr Ile  Val Asn Phe 
    1130                 1135                 1140             


Lys Val  Asn Lys Arg Ile Ile  Asn Lys Thr Ile Asn  Trp Tyr Lys 
    1145                 1150                 1155             


Lys Trp  Glu Lys Gln Lys Asp  Gly Thr Tyr Lys Lys  Ile Gln Lys 
    1160                 1165                 1170             


Lys Gln  Thr Lys Gly Asp Ala  Trp Ala Ile Arg Lys  Pro Leu His 
    1175                 1180                 1185             


Ile Asp  Thr Ile Tyr Gly Ile  Val Cys Lys Lys Leu  Pro Leu Lys 
    1190                 1195                 1200             


Ser Ala  Leu Glu Asn Tyr Lys  Asn Ile Ile Asp Glu  Asn Leu Lys 
    1205                 1210                 1215             


His Lys  Ile Lys Glu Leu Lys  Glu Val Asn Lys Phe  Asp Asn Asn 
    1220                 1225                 1230             


Gln Ile  Ile Asn Phe Phe Lys  Glu Cys Asn Phe Ile  Phe Glu Asp 
    1235                 1240                 1245             


Asn Asn  Ile Gln Lys Val Glu  Val Tyr Asp Phe Lys  Asp Asn Arg 
    1250                 1255                 1260             


Asp Lys  Thr Ile Leu Ser Ser  Ser Arg Gln Leu Ile  Asp Glu Ser 
    1265                 1270                 1275             


Phe Thr  Pro Lys Lys Ile Gly  Lys Val Leu Asp Pro  Thr Ile Arg 
    1280                 1285                 1290             


Asn Ile  Leu Leu Lys His Leu  Asn Ser Thr Lys Tyr  Asn Asn Lys 
    1295                 1300                 1305             


Phe Asp  Ser Asp Gly Asn Pro  Ile Lys Pro Glu Lys  Leu Ala Phe 
    1310                 1315                 1320             


Ser Pro  Glu Gly Leu Lys Asp  Leu Asn Lys Asn Ile  Lys His Leu 
    1325                 1330                 1335             


Asn Asn  Asn Lys Glu His Gln  Pro Ile Lys Arg Val  Lys Leu Phe 
    1340                 1345                 1350             


Glu Thr  Leu Gly Asn Lys Ile  Lys Val Gly Tyr Thr  Gln Gln Lys 
    1355                 1360                 1365             


Asn Lys  Lys Phe Ala Glu Thr  Ala Thr Gly Thr Asn  Leu Phe Leu 
    1370                 1375                 1380             


Gly Ile  Tyr Ser Asp Asn Asn  Lys Arg Asp Phe Lys  Thr Ile Pro 
    1385                 1390                 1395             


Leu Ile  Leu Ala Ile Gln Arg  Glu Lys Glu Gly Leu  Asn Pro Cys 
    1400                 1405                 1410             


Pro Asp  Thr Asp Glu Lys Gly  Asn Gln Leu Leu Phe  Ser Leu Ser 
    1415                 1420                 1425             


Pro Asn  Glu Leu Val Phe Val  Pro Asn Asn Glu Glu  Arg Lys Asn 
    1430                 1435                 1440             


Pro Asn  Leu Val Asp Phe Glu  Asn Leu Ser Lys Asp  Gln Ile Asp 
    1445                 1450                 1455             


Arg Ile  Tyr Lys Phe Val Ser  Cys Thr Gly Ser Glu  Ala Tyr Phe 
    1460                 1465                 1470             


Val Pro  Ser Arg Thr Ala Lys  Pro Ile Ile Glu Asn  Glu Asn Gly 
    1475                 1480                 1485             


Thr Asn  Asn Lys Ser Gln Arg  Ile Ile Glu Phe Asn  Glu Lys Thr 
    1490                 1495                 1500             


Ser Ile  Lys Asn Glu Lys Asn  Glu Pro Val Leu Ile  Lys Thr Ile 
    1505                 1510                 1515             


Cys Trp  Lys Ile Glu Val Asn  Arg Leu Gly Lys Ile  Ile Lys Val 
    1520                 1525                 1530             


Ile Lys  
    1535 


<210>  1336
<211>  1535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1336

Met Thr Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Ile Arg Asp Thr Glu Asn Glu Gly Ile Asn Gln Ile Leu Asp Lys 
            20                  25                  30          


Gly Ala Arg Ile Phe Ser Glu Gly Val Lys Ser Glu Asn Gly Lys Glu 
        35                  40                  45              


Ile Ser Arg Ala Ala Glu Arg Thr Ala Tyr Arg Ser Ala Arg Lys Ile 
    50                  55                  60                  


Lys Tyr Arg Arg Lys Leu Arg Lys Tyr Glu Thr Leu Lys Val Leu Ser 
65                  70                  75                  80  


Ile Asn Gly Met Cys Pro Leu Ser Val Glu Glu Val Glu Gln Trp Lys 
                85                  90                  95      


Lys Ser Gly Phe Lys Glu Tyr Pro Leu Asn Pro Glu Phe Leu Asp Trp 
            100                 105                 110         


Leu Arg Thr Asn Glu Asp Lys Asn Ile Asn Pro Tyr Leu Phe Arg Asp 
        115                 120                 125             


Lys Ala Ser Lys Gln Lys Val Thr Leu Phe Glu Leu Gly Arg Ala Leu 
    130                 135                 140                 


Tyr His Ile Ala Gln Arg Arg Gly Phe Leu Ser Asn Arg Leu Asp Gln 
145                 150                 155                 160 


Ser Ala Glu Gly Val Phe Glu Glu His Asn Pro Gln Ile Gln Asn Leu 
                165                 170                 175     


Ile Glu Asp Leu Asp Ser Ser Asn Thr Ile Leu Asn Glu Leu Lys Glu 
            180                 185                 190         


Tyr Tyr Ile Asn Leu Gly Ile Ile Asp Glu Thr Glu Lys Ser Gly Phe 
        195                 200                 205             


Lys Lys Asp Leu Asp Glu Gly Glu Lys Lys Leu Lys Ser Leu Tyr Asn 
    210                 215                 220                 


Ser Leu Val Ala Ile Thr Lys Lys Asn Ala Asn Asp Ile Glu Thr Cys 
225                 230                 235                 240 


Lys Gln Glu Leu Ile Ala Arg Leu Asn Lys Lys Glu Asp Leu Gly Lys 
                245                 250                 255     


Val Lys Gly Lys Ile Lys Asp Ile Ser Gln Ala Met Leu Asp Arg Asn 
            260                 265                 270         


Phe Lys Thr Leu Gly Gln Tyr Phe Phe Ser Leu Tyr Asn Lys Glu Arg 
        275                 280                 285             


Ile Arg Asn Gln Tyr Thr Ser Arg Glu Glu His Tyr Leu Glu Glu Phe 
    290                 295                 300                 


Ile Ile Ile Cys Gln Thr Gln Gly Ile Glu Gly Ile Asn Thr Asn Glu 
305                 310                 315                 320 


Lys Leu Pro Glu Lys Lys Phe Thr Gly Leu Ala Lys Asp Leu Tyr Arg 
                325                 330                 335     


Ala Ile Phe Phe Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Ile Gly 
            340                 345                 350         


Lys Cys Ser Phe Glu Lys Asn Lys Ser Arg Cys Ala Val Ser His Pro 
        355                 360                 365             


Asp Tyr Glu Glu Phe Arg Met Trp Ser Tyr Leu Asn Thr Ile Lys Ile 
    370                 375                 380                 


Gly Thr Gln Ser Glu Lys Thr Leu Arg Phe Leu Thr Leu Glu Glu Lys 
385                 390                 395                 400 


Leu Lys Leu Val Pro Lys Phe Tyr Arg Lys Ser Asp Phe Asn Phe Glu 
                405                 410                 415     


Val Leu Ala Lys Glu Leu Val Glu Lys Gly Ala Ser Phe Gly Tyr Tyr 
            420                 425                 430         


Lys Ser Ser Lys Lys Asn Glu Phe Phe Tyr Trp Phe Asn Tyr Lys Pro 
        435                 440                 445             


Thr Asp Ser Val Ser Ala Cys Val Val Ser Ala Ser Leu Glu Asn Ala 
    450                 455                 460                 


Ile Gly Lys Asp Trp Lys Ile Lys Thr Phe Glu Tyr Gln Thr Arg Asn 
465                 470                 475                 480 


Thr Glu Lys Asn Glu Val Thr Lys Ser Val Asp Tyr Lys Asp Leu Trp 
                485                 490                 495     


His Leu Leu Ser Val Ala Thr Ser Asp Thr Tyr Leu Tyr Asp Phe Ala 
            500                 505                 510         


Ile Glu Lys Leu Lys Leu Glu Pro Lys Asn Ala Lys Ala Phe Ser Lys 
        515                 520                 525             


Thr Lys Leu Lys Lys Asp Phe Ala Ser Leu Ser Leu Ala Ala Ile Ala 
    530                 535                 540                 


Lys Ile Leu Pro Tyr Leu Lys Gln Gly Leu Leu Tyr Ser His Ala Val 
545                 550                 555                 560 


Phe Met Ala Asn Ile Glu Asn Ile Val Asp Ala Asp Ile Trp Lys Asp 
                565                 570                 575     


Glu Glu Gln Gln Lys Phe Ile Gln Ser Lys Ile Val Glu Leu Val Asp 
            580                 585                 590         


Asn Tyr Ile Val Glu Lys Ser Lys Leu Glu Leu Ile Asn Gly Leu Leu 
        595                 600                 605             


Lys Ile Tyr Asn Thr Glu Asp Lys Glu Gly Arg Lys Val Tyr Tyr Ser 
    610                 615                 620                 


Lys Glu Ala Glu Ser Leu Phe Glu Ala Asp Leu Arg Lys Lys Leu Val 
625                 630                 635                 640 


Pro Phe Tyr Lys Ala Asn Ile Ile Ile Glu Glu His Glu Gln Glu Ile 
                645                 650                 655     


Ile Phe Gln Asp Leu Phe Pro Ile Leu Met Asp Gln Leu Lys Lys Gln 
            660                 665                 670         


Glu Phe Ile Lys Ile Gly Arg Leu Asp Lys Gln Ile Glu Ala Phe Leu 
        675                 680                 685             


Glu Gly Glu Asn Glu Glu Gly Gln Ile Phe Cys Asn His Thr Asp Lys 
    690                 695                 700                 


Leu Lys Lys Leu Tyr His Pro Ser Asp Ile Glu Val Phe Lys Lys Lys 
705                 710                 715                 720 


Thr Ile Lys Asp Glu Trp Gly Asn Glu Lys Val Val Leu Gly Ser Pro 
                725                 730                 735     


Leu Thr Thr Ser Ile Lys Asn Pro Met Ala Met Arg Ala Leu His Gln 
            740                 745                 750         


Leu Arg Lys Val Leu Asn Thr Leu Ile Ala Asn Asp Gln Ile Asp Glu 
        755                 760                 765             


Asp Thr Arg Ile His Ile Glu Met Ala Arg Glu Leu Asn Asp Ala Asn 
    770                 775                 780                 


Lys Arg Lys Gly Ile Gln Asp Phe Gln Asn Glu Asn Lys Lys Phe Arg 
785                 790                 795                 800 


Glu Glu Ala Ile Lys Glu Ile Lys Lys Leu Tyr Leu Glu Glu Cys His 
                805                 810                 815     


Lys Asp Val Asn Pro Thr Glu Asp Asp Ile Leu Arg Tyr Gln Leu Trp 
            820                 825                 830         


Leu Glu Gln Gly Lys Cys Glu Ile Tyr Glu Glu Gly Asn Asn Ile Ser 
        835                 840                 845             


Ile Cys Asp Ile Ile Gly Ser Asn Pro Ser Tyr Asp Ile Glu His Thr 
    850                 855                 860                 


Ile Pro Arg Ser Ile Ser Gln Asp Asn Ser Gln Met Asn Lys Thr Leu 
865                 870                 875                 880 


Cys Ser Leu Lys Phe Asn Arg Ser Ile Lys Lys Gln Lys Met Pro Val 
                885                 890                 895     


Glu Leu Ser Asn Tyr Asn Asp Ile Leu Pro Arg Ile Ala His Trp Lys 
            900                 905                 910         


Lys Glu Ala Glu Glu Leu Thr Arg Gln Ile Glu Thr Ile Ser Arg Ser 
        915                 920                 925             


Ile Lys Ser Ala Ala Thr Lys Val Ala Lys Asp Lys Asn Ile Arg Lys 
    930                 935                 940                 


Arg His Tyr Leu Thr Leu Lys Arg Asp Tyr Ile Leu Gly Lys Tyr Glu 
945                 950                 955                 960 


Arg Phe Thr Trp Glu Glu Pro Lys Val Gly Phe Lys Asn Ser Gln Ile 
                965                 970                 975     


Pro Asp Thr Gly Ile Ile Thr Lys Tyr Ala Gln Ala Tyr Leu Lys Ser 
            980                 985                 990         


Tyr Phe Lys Arg Val Glu Ser Val  Lys Gly Gly Ala Val  Ala Glu Phe 
        995                 1000                 1005             


Arg Lys  Leu Trp Gly Ile Gln  Glu Ser Phe Ile Asp  Glu Asn Trp 
    1010                 1015                 1020             


Trp Lys  His Tyr Lys Asp Lys  Asp Arg Asp Lys His  Thr His His 
    1025                 1030                 1035             


Thr Ile  Asp Ala Ile Thr Ile  Ala Cys Met Pro Lys  Asp Lys Tyr 
    1040                 1045                 1050             


Asp Leu  Leu Ala His Ala Trp  Arg Leu Glu Asp Glu  Gln Asp Lys 
    1055                 1060                 1065             


Lys Ala  Ala Lys Val Leu Ile  Glu Gln Ala Lys Pro  Trp Lys Thr 
    1070                 1075                 1080             


Phe Lys  Glu Asp Ile Glu Lys  Ile Glu Thr Glu Ile  Leu Val Ser 
    1085                 1090                 1095             


His Phe  Thr Pro Asp Asn Val  Lys Lys Gln Ser Lys  Ser Ile Ile 
    1100                 1105                 1110             


Lys Asn  Arg Gly Lys Lys Val  Tyr Val Leu Lys Asn  Glu Leu Pro 
    1115                 1120                 1125             


Val Asn  Phe Lys Asn Lys Ile  Glu Gly Lys Asp Tyr  Phe Lys Leu 
    1130                 1135                 1140             


Lys Phe  Asp Ser Lys Ile Leu  Tyr Lys Ile Pro Lys  Lys Lys Glu 
    1145                 1150                 1155             


Lys Gln  Thr Asp Thr Phe Tyr  Glu Glu Leu Pro Lys  Asn Tyr Leu 
    1160                 1165                 1170             


Asn Gly  Val Glu Gly Lys Asp  Tyr Phe Lys Ile Asn  Thr Thr Gly 
    1175                 1180                 1185             


Lys Thr  Phe Tyr Lys Ile Pro  Ile Phe Asn Gln Gly  Asp Thr Ile 
    1190                 1195                 1200             


Arg Gly  Ser Leu His Gln Glu  Thr Thr Tyr Gly Ala  Ile Lys Leu 
    1205                 1210                 1215             


Pro Asp  Ile Asp Ile Glu Thr  Lys Lys Pro Leu His  Thr Asp Lys 
    1220                 1225                 1230             


Gly Gly  Phe Ile Leu Lys Lys  Asp Ile Lys Gly Asn  Glu Ile Val 
    1235                 1240                 1245             


Phe Phe  Val Val Arg Lys Glu  Ile Ser Lys Ile Ser  Glu Asn Asp 
    1250                 1255                 1260             


Val Gln  Asn Ile Val Asp Asn  Val Val Arg Lys Lys  Ile Glu Asn 
    1265                 1270                 1275             


Ala Ile  Ala Asn Ser Leu Ile  Thr Phe Lys Ile Val  Lys Lys Lys 
    1280                 1285                 1290             


Lys Val  Ala Val Ile Lys Glu  Gly Val Thr Ile Trp  Met Arg Glu 
    1295                 1300                 1305             


Pro Asn  Ile Glu Lys Gly Ile  Glu Gly Ile Pro Ile  Lys Lys Val 
    1310                 1315                 1320             


Arg Ile  Ile Thr Asn Ser Val  Lys Asn Pro Ile Glu  Ile Lys Val 
    1325                 1330                 1335             


His Ser  Pro Leu Ser Lys Ser  Arg His Lys His Lys  Gln Lys Val 
    1340                 1345                 1350             


Tyr Gly  Gln Asn Asp Glu Asn  Tyr Ala Met Ala Leu  Tyr Glu Leu 
    1355                 1360                 1365             


Asp Gly  Lys Arg Glu Phe Glu  Leu Ile Asn Asn Phe  Asn Leu Ala 
    1370                 1375                 1380             


Lys Leu  Leu Lys Gln Ser Gln  Ser Tyr Tyr Pro Leu  His Lys Glu 
    1385                 1390                 1395             


Lys Glu  Ile Lys Gly Lys Lys  Ile Leu Val Pro Ile  Glu Lys Arg 
    1400                 1405                 1410             


Asn Asn  Lys Asp Val Ile Leu  Lys Arg Gly Gln Gln  Val Val Phe 
    1415                 1420                 1425             


Tyr Asp  Lys Thr Val Glu Asn  Pro Lys Asp Ile Ser  Glu Ile Ile 
    1430                 1435                 1440             


Asp Phe  Arg Glu Arg Ile Tyr  Ile Ile Glu Gly Leu  Thr Ile Gln 
    1445                 1450                 1455             


Arg Gln  Lys Asp Lys Lys Thr  Ser Lys Val Asn Glu  Tyr Gly Ile 
    1460                 1465                 1470             


Ile Gln  Leu Arg His Phe Lys  Glu Ala Arg Lys Ser  Glu Glu Ile 
    1475                 1480                 1485             


Ser Lys  Asp Asn Phe Lys Pro  Asp Gly Glu Phe Lys  Ile Asn Glu 
    1490                 1495                 1500             


Asn Lys  Pro Thr Arg Lys Met  Asn His Asn Gln Phe  Thr Ala Phe 
    1505                 1510                 1515             


Val Glu  Gly Ile Asp Phe Lys  Val Leu Pro Ser Gly  Lys Phe Gln 
    1520                 1525                 1530             


Lys Ile  
    1535 


<210>  1337
<211>  1537
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1337

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Thr Thr Asn Asp Phe Asp Thr Lys Lys Gly Thr Ile Glu Gly 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Ile Leu Gly Lys Phe 
        35                  40                  45              


Asp Ser Gly Val Ser Leu Ser Gln Thr Ala Gln Arg Thr Glu Tyr Arg 
    50                  55                  60                  


Gly Lys Arg Arg Leu Val Gln Arg Phe Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Ile Leu Glu Leu Leu Pro Lys Gln Tyr Ser Glu 
                85                  90                  95      


Ala Ile Asp Phe Asp Lys Lys Leu Gly Gln Phe Lys Pro Glu Arg Glu 
            100                 105                 110         


Gln Lys Ile Ser Phe Tyr Lys Asn Glu Ser Gly Lys Asn Gln Phe Ile 
        115                 120                 125             


Phe Thr Lys Ser His Gln Glu Met Leu Thr Glu Phe Gln Thr Tyr Tyr 
    130                 135                 140                 


Pro Thr Ile Lys Asn Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg 
145                 150                 155                 160 


Lys Lys Ala Leu Thr Glu Lys Ile Ser Lys Glu Glu Leu Ala Trp Ile 
                165                 170                 175     


Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu 
            180                 185                 190         


Glu Lys Glu Glu Asn Lys Asn Lys Lys Glu Glu Phe His Thr Leu Leu 
        195                 200                 205             


Val Thr Asp Ile Ile Glu Arg Asp Lys Gly Lys Ser Gly Thr Trp Tyr 
    210                 215                 220                 


Asn Val Ile Leu Glu Asn Gly Trp Ile Tyr Lys Arg Glu Ser Lys Ile 
225                 230                 235                 240 


Ser Leu Ala Asp Trp Ile Gly Lys Thr Lys Ser Phe Ile Val Thr Thr 
                245                 250                 255     


Glu Tyr Asn Asp Asp Gly Ser Val Lys Leu Thr Lys Asp Asn Glu Glu 
            260                 265                 270         


Lys Arg Ser Phe Arg Ser Pro Lys Glu Asp Asp Trp Gly Leu Ile Lys 
        275                 280                 285             


Ile Arg Thr Glu Lys Thr Ile Asn Gln Ser Asp Lys Ser Val Gly Ala 
    290                 295                 300                 


Tyr Ile Tyr Asp Ala Leu Leu Lys Asn Pro Asn Gln Lys Ile Lys Gly 
305                 310                 315                 320 


Glu Leu Val Arg Thr Ile Glu Arg Lys Tyr Tyr Lys Asp Glu Leu Arg 
                325                 330                 335     


Glu Ile Leu Lys Lys Gln Leu Glu Phe His Ser Asp Val Leu Asn Asp 
            340                 345                 350         


Lys Ile Leu Tyr Lys Lys Cys Ile Asp Glu Leu Tyr Lys Asn Asn Asp 
        355                 360                 365             


Ser His Lys Asn Ser Ile Lys Asp Arg Gly Phe Asp Tyr Leu Phe Leu 
    370                 375                 380                 


Asp Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys Thr Ser Leu 
385                 390                 395                 400 


Ile Ser Asp Cys Ser Leu Glu Phe Arg Pro Ile Lys Asn Lys Asn Gly 
                405                 410                 415     


Val Leu Val Lys Asp Thr Asp Gly Lys Gln Ile Ala Lys Ala Leu Lys 
            420                 425                 430         


Cys Ile Ala Arg Ser Asn Pro Ile Phe Gln Glu Phe Arg Leu Leu Gln 
        435                 440                 445             


Phe Val Lys Asn Leu Lys Ile Tyr Lys Arg Gly Val Val Asp Asp Val 
    450                 455                 460                 


Glu Val Thr Asn Asp Tyr Leu Lys Ser Glu Asn Asp Val Glu Lys Leu 
465                 470                 475                 480 


Phe Asn Trp Leu Asn Asp Lys Ala Glu Ile Asn Gln Lys Gln Phe Leu 
                485                 490                 495     


Val Asn Phe Lys Leu Lys Glu Asp Lys His Arg Trp Asn Phe Val Glu 
            500                 505                 510         


Asp Lys Gly Tyr Pro Cys Asn Glu Thr Arg Asn Gln Phe Leu Asn Ala 
        515                 520                 525             


Ile Ala Lys Ile Asp Val Asp Lys Ser Phe Phe Asn Thr Lys Asn Thr 
    530                 535                 540                 


Gln Asp Leu Trp His Ile Leu Tyr Ser Val Thr Asp Lys Ile Glu Ile 
545                 550                 555                 560 


Thr Lys Ala Ile Thr Thr Phe Ala Lys Arg His Asn Leu Pro Asn Glu 
                565                 570                 575     


Phe Ile Ile Asn Phe Ser Lys Ile Lys Pro Tyr Lys Lys Glu Tyr Gly 
            580                 585                 590         


Ser Phe Ser Glu Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Phe 
        595                 600                 605             


Gly Ser Tyr Trp Asp Ala Lys Asn Ile Asp Ile Lys Thr Leu Asp Arg 
    610                 615                 620                 


Ile Asp Lys Ile Gln Thr Gly Glu Phe Asp Glu Lys Ile Arg Asn Arg 
625                 630                 635                 640 


Val Arg Glu Lys Ala Ile Asp Leu His Lys Ile Asp Asp Phe Lys Asp 
                645                 650                 655     


Leu Pro Leu Trp Leu Ala Ser Tyr Ile Val Tyr Asp Lys His Ser Glu 
            660                 665                 670         


Ala Ser Asp Val Ser Lys Trp Lys Thr Pro Ala Asp Ile Glu Lys Phe 
        675                 680                 685             


Leu Lys His Glu Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu 
    690                 695                 700                 


Gln Val Ile Thr Glu Thr Leu Arg Val Val Lys Asp Ile Trp Leu Gln 
705                 710                 715                 720 


Tyr Gly Glu Gly Lys Glu Asn Phe Phe Asn Glu Ile His Ile Glu Leu 
                725                 730                 735     


Gly Arg Glu Met Lys Asn Asp Lys Ala Thr Arg Glu Lys Ile Ser Lys 
            740                 745                 750         


Lys Val Ser Glu Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala Ile Leu 
        755                 760                 765             


Thr Glu Leu Lys Asn Asp Gly Val Ser Asp Ile Arg Pro Tyr Ser Pro 
    770                 775                 780                 


Ser Gln Gln Glu Ile Leu Lys Ile Tyr Glu Glu Gly Val Tyr Asn Asn 
785                 790                 795                 800 


Glu Ser Arg Lys Glu Lys Leu Glu Glu Val Asp Lys Ile Arg Lys Asn 
                805                 810                 815     


Ala Lys Pro Thr Thr Ser Glu Ile Ile Lys Tyr Lys Leu Trp Leu Glu 
            820                 825                 830         


Gln Gly Tyr Ala Ser Pro Tyr Thr Gly Lys Leu Ile Pro Met Ser Glu 
        835                 840                 845             


Leu Phe Thr Thr Lys Tyr Gln Ile Glu His Ile Ile Pro Gln Ser Arg 
    850                 855                 860                 


Phe Phe Asp Asp Ser Leu Ser Asn Lys Val Ile Cys Glu Ser Glu Val 
865                 870                 875                 880 


Asn Glu Leu Lys Ser Asn Met Thr Ala Leu Glu Phe Ile Leu Thr Asn 
                885                 890                 895     


Glu Asn Arg Ile Val Thr Leu Thr Gly Gly Glu Thr Val Lys Ile Phe 
            900                 905                 910         


Thr Lys Glu Ala Tyr Asn Gln His Ile Ile Ser Tyr Tyr Ser Lys Asn 
        915                 920                 925             


Lys Asn Lys Gln Lys Arg Leu Leu Thr Glu Glu Ile Pro Glu Gln Phe 
    930                 935                 940                 


Ile Ser Arg Gln Leu Asn Asp Thr Lys Tyr Ile Ser Lys Ile Val Lys 
945                 950                 955                 960 


Asn Leu Leu Ser Lys Ile Val Arg Glu Glu Asp Glu Gln Glu Val Thr 
                965                 970                 975     


Ser Lys His Val Val Ser Leu Asn Gly Ser Ile Thr Ser Arg Met Lys 
            980                 985                 990         


Gln Asp Trp Gly Leu Asn Asp Val  Trp Asn Thr Ile Val  Ser Pro Arg 
        995                 1000                 1005             


Phe Glu  Arg Leu Asn Thr Leu  Thr Asn Ser Asn Asp  Phe Gly Gln 
    1010                 1015                 1020             


Phe Glu  Leu Lys Lys Asp Ala  Tyr Gly Asn Glu Gly  Lys Arg Val 
    1025                 1030                 1035             


Phe Gln  Thr Thr Val Pro Asp  Ala Ile Ala Lys Gly  Phe Thr Lys 
    1040                 1045                 1050             


Lys Arg  Ile Asp His Arg His  His Ala Leu Asp Ala  Leu Val Ile 
    1055                 1060                 1065             


Ala Cys  Val Asn Arg Thr His  Ile Asn Tyr Leu Asn  Asn Leu Asn 
    1070                 1075                 1080             


Ala Lys  Asp Thr Asp Asp Lys  Ala Val Lys His Glu  Leu Arg Asn 
    1085                 1090                 1095             


Lys Leu  Cys Phe Lys Thr Lys  Pro Asp Ser Asn Gly  Asn Tyr Lys 
    1100                 1105                 1110             


Trp Glu  Phe His Lys Pro Trp  Glu Lys Phe Thr Lys  Glu Ala Gln 
    1115                 1120                 1125             


Asp Lys  Leu Asn Thr Thr Ile  Val Ser Phe Lys Gln  Asn Thr Arg 
    1130                 1135                 1140             


Val Ile  Asn Lys Thr Val Asn  Lys Tyr Val Ser Tyr  Lys Asp Glu 
    1145                 1150                 1155             


Asn Gly  Asn Leu Asn Val Asp  Ala Asn Gly Leu Pro  Gln Lys Lys 
    1160                 1165                 1170             


Val Ile  Thr Gln Thr Lys Gly  Asp Asn Trp Ala Ile  Arg Lys Pro 
    1175                 1180                 1185             


Leu His  Ala Glu Thr Val Ser  Gly Lys Val Phe Leu  Lys Arg Ile 
    1190                 1195                 1200             


Lys Gln  Ser His Ile Thr Ile  Ala Asn Ala Ile Glu  Gln Val Glu 
    1205                 1210                 1215             


Leu Ile  Val Asp Lys Glu Val  Lys Lys Gln Leu Lys  Ala Lys Ile 
    1220                 1225                 1230             


Lys Leu  Tyr Pro Asn Asn Ile  Ala Gly Leu Lys Lys  His Leu Lys 
    1235                 1240                 1245             


Ala Phe  Pro Val Met Ile Asn  Asp Lys Ala Ile Asp  Lys Ile Gln 
    1250                 1255                 1260             


Ile Tyr  Glu Thr Leu Glu Ala  Thr Ala Thr Arg Lys  Ile Leu Asp 
    1265                 1270                 1275             


Ile Thr  Phe Asp Glu Lys Lys  Ile Gln Lys Ile Thr  Asp Thr Gly 
    1280                 1285                 1290             


Ile Gln  Lys Ile Leu Leu Asn  His Leu Lys Gln Glu  Lys Tyr Gln 
    1295                 1300                 1305             


Asn Thr  Val Asp Glu Asn Gly  Lys Lys Leu Ala Pro  His Glu Ile 
    1310                 1315                 1320             


Ala Phe  Ser Glu Asn Gly Leu  Glu Asp Leu Asn Lys  Asn Ile Thr 
    1325                 1330                 1335             


Phe Leu  Asn Asp Gly Lys Asn  His Gln Pro Ile Lys  Lys Val Arg 
    1340                 1345                 1350             


Ile Phe  Glu Glu Gly Asn Lys  Phe Pro Leu Gly Glu  Val Gly Asn 
    1355                 1360                 1365             


Lys Ile  Asn Lys Phe Val Glu  Thr Ala Lys Gly Thr  Asn Leu Phe 
    1370                 1375                 1380             


Phe Ala  Val Tyr Glu Asn Asp  Asp Lys Lys Arg Asn  Tyr Lys Thr 
    1385                 1390                 1395             


Ile Pro  Phe Asn Glu Val Ile  Glu Arg Gln Lys Gln  Gly Leu Asn 
    1400                 1405                 1410             


Ala Ala  Leu Glu Ile Asp Glu  Asn Gly Asn Arg Leu  Leu Phe Thr 
    1415                 1420                 1425             


Leu Ser  Pro Asn Asp Leu Val  Tyr Ile Pro Leu Glu  Asp Glu Ile 
    1430                 1435                 1440             


Gly Tyr  Leu Asn Gly Val Tyr  Phe Lys Asn Leu Asn  Lys Glu Gln 
    1445                 1450                 1455             


Thr Val  Arg Ile Tyr Lys Phe  Val Ser Cys Thr Gly  Gly Glu Gly 
    1460                 1465                 1470             


His Phe  Val Pro Asn Asn Tyr  Ser Lys Glu Ile Ile  Ser Asn Glu 
    1475                 1480                 1485             


Asn Gly  Ser Asn Asn Lys Asn  Glu Arg Met Leu Asn  Phe Asn Asn 
    1490                 1495                 1500             


Phe Asn  Ile Ile Phe Asp Gly  Lys Glu Lys Pro Val  Met Ile Lys 
    1505                 1510                 1515             


Ser Ile  Cys Trp Lys Leu Glu  Val Asp Arg Leu Gly  Lys Val Ile 
    1520                 1525                 1530             


Lys Ile  Ile Lys 
    1535         


<210>  1338
<211>  1538
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1338

Met Lys His Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Lys Ser Glu Gln Asp Cys Asp Asp Val Glu Arg Ile Asn 
            20                  25                  30          


Gly Ile Glu Ala Ala Gly Ser Arg Ile Ile Pro Met Asp Ala Ala Val 
        35                  40                  45              


Leu Gly Asp Phe Ala Lys Gly Asn Thr Lys Ser Gln Thr Ala Asp Arg 
    50                  55                  60                  


Thr Ala Ala Arg Gly Ile Arg Arg Met Gly Glu Arg His Lys Leu Arg 
65                  70                  75                  80  


Arg Glu Arg Leu Asn Arg Val Leu Asp Val Met Gly Phe Leu Pro Glu 
                85                  90                  95      


His Tyr Ser Ala Ala Leu Thr Arg Tyr Gly Lys Phe Lys Asp Gly Glu 
            100                 105                 110         


Glu Cys Lys Ile Ala Trp Arg Lys Asp Glu Ser Gly Gln Trp Thr Phe 
        115                 120                 125             


Val Phe Glu Asp Ser Phe Arg Glu Met Leu Ala Asp Phe Val Ala His 
    130                 135                 140                 


Gln Pro Glu Trp Leu Ala Gly Gly Arg Lys Val Pro Tyr Asp Trp Thr 
145                 150                 155                 160 


Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Ser Lys Pro Leu Thr Lys Gln 
                165                 170                 175     


Glu Leu Ala Trp Leu Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr 
            180                 185                 190         


Gln Leu Arg Gly Glu Asp Glu His Thr Asp Asp Lys Gly Lys Leu Val 
        195                 200                 205             


Glu Tyr Tyr Ala Leu Lys Val Val Gly Val Glu Asp Thr Gly Asp Lys 
    210                 215                 220                 


Lys Gly Lys Asp Thr Trp Phe Asn Ile Met Leu Glu Asn Gly Met Val 
225                 230                 235                 240 


Tyr Arg Arg Pro Ala Ala Glu Ala Pro Asp Trp Ile Gly Lys Thr Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Asp Leu Glu Lys Asp Gly Thr Pro Lys Lys 
            260                 265                 270         


Asp Lys Glu Gly Asn Ile Lys Arg Ser Phe Arg Met Pro Lys Glu Asp 
        275                 280                 285             


Asp Trp Thr Leu Ile Lys Lys Lys Thr Glu Asn Asp Ile Asp Arg Ser 
    290                 295                 300                 


Gly Lys Thr Val Gly Glu Tyr Ile Tyr Asp Ala Leu Leu Ala Asp Pro 
305                 310                 315                 320 


Thr Gln Lys Ile Arg Gly Lys Leu Val Arg Thr Val Glu Arg Lys Tyr 
                325                 330                 335     


Tyr Lys Glu Glu Leu Lys Lys Ile Ile Asn Ser Gln Lys Arg Phe Ile 
            340                 345                 350         


Pro Glu Leu Asn Asp Asn Glu Leu Tyr Gly Arg Cys Ile Ser Glu Leu 
        355                 360                 365             


Tyr Gln Ser Asn Asp Ala Tyr Arg Gln Ser Ile Ser Ser Cys Asp Phe 
    370                 375                 380                 


Thr Tyr Leu Phe Val Asp Asn Ile Ile Phe Tyr Gln Arg Pro Leu Lys 
385                 390                 395                 400 


Ser Lys Lys Ser Leu Ile Asp Asn Cys Pro Tyr Glu Glu His Ser Tyr 
                405                 410                 415     


Thr Asp Ser Lys Thr Gly Glu Val Lys Thr Ala Pro Val Lys Cys Val 
            420                 425                 430         


Ala Lys Ser Asn Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Val 
        435                 440                 445             


Gln Asn Ile Arg Ile Phe Glu Arg Gln Lys Lys Ile Gly Asp Ser Val 
    450                 455                 460                 


Lys Thr Asp Val Asp Val Thr Gly Glu Phe Ile Lys Ser Ala Asp Asp 
465                 470                 475                 480 


Val Val Ser Leu Phe Asp Phe Leu Asn Asp Arg Lys Asp Ile Thr Gln 
                485                 490                 495     


Ser Val Leu Leu Gly Thr Tyr Phe Lys Ile Lys Lys Pro Lys Gly Lys 
            500                 505                 510         


Asp Ser Asp Tyr Pro Tyr Arg Trp Asn Tyr Val Glu Asp Lys Thr Tyr 
        515                 520                 525             


Pro Cys Asn Glu Thr Arg Ala Leu Ile Leu Lys Gly Leu Lys Asp Ala 
    530                 535                 540                 


Gly Ile Asp Glu Gly Phe Leu Asp Lys Asp Lys Glu Tyr Ser Leu Trp 
545                 550                 555                 560 


His Ile Leu Tyr Ser Val Glu Asp Lys Gln Glu Ile Thr Lys Ala Met 
                565                 570                 575     


Glu Lys Phe Ala Ala Lys Asn Ser Leu Gly Gly Lys Phe Ala Glu Val 
            580                 585                 590         


Phe Ser Ser Leu Pro Pro Phe Asn Lys Asn Tyr Gly Ser Tyr Ser Glu 
        595                 600                 605             


Lys Ala Ile Lys Lys Leu Leu Ala Leu Met Arg Thr Gly Lys Tyr Trp 
    610                 615                 620                 


Ser Ala Asp Ala Ile Asp Lys His Thr Leu Glu Arg Ile Asp Lys Ile 
625                 630                 635                 640 


Ile Asn Gly Glu Tyr Asp Glu Ser Ile Arg Thr Arg Val Arg Glu Lys 
                645                 650                 655     


Ala Ile Asn Leu Thr Asp Val Ser His Phe Arg Gly Leu Pro Leu Trp 
            660                 665                 670         


Leu Ala Cys Tyr Ile Val Tyr Asp Arg His Ser Glu Ser Lys Asp Ile 
        675                 680                 685             


Thr Cys Trp Asn Cys Pro Ala Asp Ile Asp Gln Tyr Leu Asp His Phe 
    690                 695                 700                 


Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val Thr Glu 
705                 710                 715                 720 


Thr Leu Arg Thr Val Arg Asp Ile Trp Arg Gln Tyr Gly Lys Ile Asp 
                725                 730                 735     


Glu Ile His Ile Glu Met Gly Arg Asp Met Lys Asn Pro Ala Asp Val 
            740                 745                 750         


Arg Ala Lys Met Ser Lys Arg Met Ile Asp Asn Glu Asn Ala Asn Leu 
        755                 760                 765             


Arg Ile Lys Ala Met Leu Ala Glu Phe Ala Asp Pro Asp Phe Gly Ile 
    770                 775                 780                 


Ala Gly Val Arg Pro Tyr Ser Pro Ser Gln Gln Glu Met Leu Arg Ile 
785                 790                 795                 800 


Tyr Glu Glu Gly Ala Leu Glu Ser Val Asp Glu Val Pro Asp Glu Ile 
                805                 810                 815     


Asn Glu Ile Ile Ser Lys Phe Asn Gln Thr Asp Ile Lys Lys Arg Pro 
            820                 825                 830         


Thr His Gly Glu Ile Ile Lys Tyr Lys Leu Trp Leu Glu Gln Lys Tyr 
        835                 840                 845             


Gln Ser Pro Tyr Thr Gly Arg Thr Ile Ser Leu Ser Arg Leu Phe Thr 
    850                 855                 860                 


Asp Asp Tyr Gln Ile Glu His Val Ile Pro Gln Ser Arg Tyr Phe Asp 
865                 870                 875                 880 


Asp Ser Met Ser Asn Lys Val Ile Cys Glu Ala Glu Val Asn Gln Leu 
                885                 890                 895     


Lys Asp Arg Met Leu Gly Tyr Glu Phe Ile Lys Ala His His Gly Glu 
            900                 905                 910         


Lys Val Leu Leu Ser Ser Gly Glu Thr Val Thr Ile Leu Thr Ala Glu 
        915                 920                 925             


Glu Tyr Glu Arg Leu Val Gln Leu Asn Phe Arg Lys Asn Pro Val Lys 
    930                 935                 940                 


Met Lys Lys Leu Leu Met Asp Asp Ile Pro Asp Lys Phe Ile Glu Arg 
945                 950                 955                 960 


Gln Leu Asn Asp Ser Arg Tyr Ile Ser Lys Lys Ile Lys Ser Leu Leu 
                965                 970                 975     


Ser Asn Met Val Arg Asp Glu Gly Glu Gln Glu Asp Ile Ser Lys Asn 
            980                 985                 990         


Val Ile Ala Cys Asn Gly Ala Ile  Thr Asp Arg Leu Lys  Arg Asp Trp 
        995                 1000                 1005             


Gly Val  Asn Asp Val Trp Asn  His Ile Ile Leu Pro  Arg Phe Glu 
    1010                 1015                 1020             


Arg Leu  Asn Ala Val Thr Gly  Ser Asp Arg Phe Thr  Thr Thr Ser 
    1025                 1030                 1035             


Thr Asn  Gly His Thr Ile Pro  Asp Met Pro Leu Asn  Leu Gln Lys 
    1040                 1045                 1050             


Gly Phe  Asn Lys Lys Arg Ile  Asp His Arg His His  Ala Met Asp 
    1055                 1060                 1065             


Ala Ile  Val Ile Ala Cys Thr  Thr Arg Ser His Val  Asn Leu Leu 
    1070                 1075                 1080             


Asn Asn  Glu Ala Ala Met Ser  Lys Asn Asn Ala Val  Arg Tyr Gln 
    1085                 1090                 1095             


Leu Ser  Arg Lys Leu Arg Arg  Tyr Glu Asp Thr Met  Val Thr Arg 
    1100                 1105                 1110             


Asn Gly  Glu Thr Arg Thr Ile  Ser Val Ala Arg Glu  Phe Leu Lys 
    1115                 1120                 1125             


Pro Trp  Pro Thr Phe Asn Ala  Asp Val Glu Lys Ala  Leu Arg Gly 
    1130                 1135                 1140             


Ile Val  Val Ser Phe Lys Gln  Asn Leu Arg Val Ile  Asn Lys Thr 
    1145                 1150                 1155             


Val Asn  Lys Tyr Gln Lys Phe  Val Tyr Asn Glu Glu  Lys His Ile 
    1160                 1165                 1170             


Lys Glu  Lys Lys Ala Val Arg  Gln Glu Glu Arg Asn  Gly Arg His 
    1175                 1180                 1185             


Leu Leu  Ala Ile Arg Lys Pro  Met His Lys Asp Thr  Val Tyr Gly 
    1190                 1195                 1200             


Glu Val  Asn Leu Arg Arg Ile  Lys Glu Val Ser Leu  Lys Asp Ala 
    1205                 1210                 1215             


Val Ala  Asn Pro Lys Ala Val  Val Asp Lys Asp Leu  Lys Lys Lys 
    1220                 1225                 1230             


Leu Met  Ala Met Ile Glu Leu  Gly Tyr Asp Leu Lys  Gln Ile Thr 
    1235                 1240                 1245             


Lys Tyr  Phe Glu Asp Asn Lys  Asp Ala Trp Gln Asp  Val Asn Leu 
    1250                 1255                 1260             


Lys Lys  Ile Lys Val Tyr Tyr  Phe Thr Lys Asp Thr  Gly Asp Arg 
    1265                 1270                 1275             


Tyr Phe  Ala Thr Arg Phe Gly  Asn Asp Leu Val Thr  Leu Phe Ser 
    1280                 1285                 1290             


Lys Ile  Thr Lys Ala Asp Asp  Ala Lys Lys Gln Ile  Glu Arg Ile 
    1295                 1300                 1305             


Thr Asp  Thr Gly Ile Gln Lys  Ile Leu Lys Arg His  Leu Glu Ala 
    1310                 1315                 1320             


Asn Gly  Gly Asp Cys Gln Lys  Ala Phe Ser Ala Asp  Gly Ile Asp 
    1325                 1330                 1335             


Glu Met  Asn Arg Asn Ile Thr  Gln Leu Asn Gly Gly  Arg Pro His 
    1340                 1345                 1350             


Lys Pro  Val Tyr Lys Val Arg  Ile Tyr Glu Lys Ala  Asp Lys Phe 
    1355                 1360                 1365             


Ala Ile  Gly Thr Lys Gly Asn  Lys Ala Ala Lys Phe  Val Glu Ala 
    1370                 1375                 1380             


Ala Lys  Gly Thr Asn Leu Phe  Phe Ala Val Tyr Leu  Lys Glu Tyr 
    1385                 1390                 1395             


Glu Asp  Ser Thr Thr Gly Glu  Thr Ile Lys Lys Arg  Val Phe Asp 
    1400                 1405                 1410             


Thr Ile  Pro Leu Lys Thr Val  Ile Asp Arg Leu Lys  Asn Gly Gln 
    1415                 1420                 1425             


Thr Pro  Ala Pro Glu Glu Lys  Asp Gly Ala Lys Leu  Leu Phe Val 
    1430                 1435                 1440             


Leu Ser  Pro Asn Asp Leu Val  Tyr Leu Pro Thr Glu  Asp Val Val 
    1445                 1450                 1455             


Lys Asn  Gly Thr Ile Asn Arg  Pro Leu Asp Lys Arg  Arg Ile Tyr 
    1460                 1465                 1470             


Lys Met  Val Ser Cys Thr Gly  Thr Glu Cys Tyr Tyr  Ile Pro Phe 
    1475                 1480                 1485             


Ile Val  Ala Ser Pro Ile Val  Asp Lys Val Glu Phe  Phe Ser Leu 
    1490                 1495                 1500             


Asn Lys  Ile Gly Arg Ala Ile  Thr Gly Glu Met Ile  Lys Glu Ile 
    1505                 1510                 1515             


Cys Ile  Pro Ile Lys Val Asp  Arg Leu Gly Asn Ile  Thr Glu Val 
    1520                 1525                 1530             


Asn Asn  Glu Lys Ile 
    1535             


<210>  1339
<211>  1540
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1339

Met Asn Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Gly Thr Asp Glu Glu Gly Asn Pro Ile Arg Ile Tyr Lys 
            20                  25                  30          


Ser Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly Asn Phe 
        35                  40                  45              


Asp Arg Gly Asn Ser Ile Ser Gln Thr Ala Glu Arg Thr Ser Tyr Arg 
    50                  55                  60                  


Gly Val Arg Arg Leu Arg Glu Arg His Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Val Leu His Leu Met Gly Phe Leu Pro Ser His Tyr Glu Glu 
                85                  90                  95      


Ala Ile Gly Trp Asp Lys Gly Asp Asn Lys Thr Phe Ala Lys Phe Tyr 
            100                 105                 110         


Ser Asp Thr Glu Pro Lys Leu Pro Trp Arg Lys Asn Ala Glu Arg Glu 
        115                 120                 125             


His Glu Phe Leu Phe Asn Thr Pro Tyr Asn Glu Met Leu Val Asp Phe 
    130                 135                 140                 


Arg Lys Asn Gln Pro Gln Leu Phe Lys Ile Lys Lys Asn Gly Gln Glu 
145                 150                 155                 160 


Ser Leu Ile Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg Lys Lys Ala 
                165                 170                 175     


Leu Ser Lys Pro Ile Ser Lys Lys Glu Leu Ala Trp Ile Ile Leu Asn 
            180                 185                 190         


Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Glu Glu Glu 
        195                 200                 205             


Glu Asn Ser Asn Lys Leu Ile Glu Tyr His Ala Leu Thr Val Val Lys 
    210                 215                 220                 


Val Ile Ala Asp Glu Pro Gln Lys Gly Lys Ser Asp Ile Trp Tyr Asn 
225                 230                 235                 240 


Ala Glu Leu Glu Asn Gly Trp Val Tyr Arg Arg Ser Ser Lys Thr Pro 
                245                 250                 255     


Leu Phe Asp Trp Glu Ser Lys Val Lys Glu Phe Val Val Thr Thr Asp 
            260                 265                 270         


Leu Asn Ala Asp Gly Ser Leu Lys Val Asp Lys Glu Gly Gly Val Lys 
        275                 280                 285             


Arg Ser Phe Arg Val Pro Ser Glu Asp Asp Trp Met Leu Val Lys Lys 
    290                 295                 300                 


Lys Thr Glu Ser Asp Ile Glu Lys Ser Asp Lys Thr Val Gly Cys Tyr 
305                 310                 315                 320 


Ile Tyr Asp Thr Ile Leu Gln Asn Pro Ser Gln Lys Ile Arg Gly Lys 
                325                 330                 335     


Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Lys Glu Leu Thr Glu 
            340                 345                 350         


Ile Leu Asn Lys Gln Lys Glu Phe His Ser Glu Leu Gln Asn Lys Glu 
        355                 360                 365             


Leu Tyr Gln Glu Cys Ile Lys Glu Leu Tyr Pro His Asn Glu Ala His 
    370                 375                 380                 


Tyr Asp Ser Leu Leu Val Lys Asp Tyr Thr Asp Leu Phe Val Asn Asp 
385                 390                 395                 400 


Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys Lys Ser Leu Ile Ser 
                405                 410                 415     


Asn Cys Pro Tyr Glu Tyr Arg Val Tyr Arg Asn Asp Ser Gly Ile Val 
            420                 425                 430         


Glu Arg Val Pro Ile Lys Cys Ile Ala Lys Ser His Pro Leu Tyr Gln 
        435                 440                 445             


Glu Phe Arg Leu Trp Gln Phe Ile His Asn Leu Arg Ile Tyr Glu Arg 
    450                 455                 460                 


Gln Arg Glu Val Asn Gly Lys Pro Glu Thr Asp Val Asp Ile Thr Ala 
465                 470                 475                 480 


Glu Phe Ile Pro Thr Thr Glu Asp Arg Val Lys Leu Phe Asp Trp Leu 
                485                 490                 495     


Asn Asn Lys Lys Glu Ile Lys Gln Asp Thr Leu Leu Gln Ser Tyr Phe 
            500                 505                 510         


Lys Ile Lys Lys Lys Lys Gly Asp Asp Lys Phe Pro Tyr Arg Trp Asn 
        515                 520                 525             


Tyr Val Glu Asp Lys Glu Tyr Pro Cys Asn Glu Thr Arg Ala Leu Ile 
    530                 535                 540                 


Leu Ser Arg Leu Thr Lys Leu Asn Leu Ser Glu Asp Phe Leu Thr Lys 
545                 550                 555                 560 


Glu Met Glu Leu Ala Leu Trp His Ile Leu Tyr Ser Val Glu Asp Lys 
                565                 570                 575     


Glu Glu Ile Lys Lys Ala Phe Lys Ser Phe Ala Gln Lys His Asn Leu 
            580                 585                 590         


Asp Asp Ser Phe Val Asp Gln Phe Ile Lys Phe Pro Pro Phe Lys Lys 
        595                 600                 605             


Asp Tyr Gly Ser Tyr Ser Ala Lys Ala Ile Lys Lys Leu Leu Pro Leu 
    610                 615                 620                 


Met Arg Ile Gly Lys Tyr Trp Asp Glu Lys Ala Ile Ser Gly Asn Thr 
625                 630                 635                 640 


Arg Ser Arg Ile Leu Ser Ile Asn Glu Arg Leu Glu Asp Met Arg Ser 
                645                 650                 655     


Ser Gly Lys Lys Ile Glu Asp Val Ser Asp Asp Asp Ile Pro Lys Gln 
            660                 665                 670         


Val Leu Lys Ser Phe Asp Lys Val Asn Gly Val Tyr Ser Gly Leu Asn 
        675                 680                 685             


Thr Tyr Gln Ala Cys Tyr Ala Val Tyr Gly Arg His Ser Glu Ala Ser 
    690                 695                 700                 


Glu Ile Thr Lys Trp Lys Thr Pro Gln Asp Ile Asp Asp Tyr Leu Asn 
705                 710                 715                 720 


Glu Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile 
                725                 730                 735     


Thr Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Lys Tyr Gly Asp 
            740                 745                 750         


Phe Ser Glu Ile His Ile Glu Leu Gly Arg Glu Met Lys Asn Pro Ala 
        755                 760                 765             


Asp Lys Arg Ala Lys Met Thr Thr Gln Ile Ser Glu Asn Glu Asn Thr 
    770                 775                 780                 


Asn Leu Arg Met Lys Ser Leu Leu Ala Glu Leu Lys Asn His Asn Asp 
785                 790                 795                 800 


Val Glu Asn Val Arg Pro Tyr Ser Pro Ser Gln Gln Glu Ile Leu Lys 
                805                 810                 815     


Ile Tyr Glu Glu Gly Val Leu Asn Ser Val Ser Glu Ile Pro Glu Glu 
            820                 825                 830         


Ile Ile Lys Ile Thr Lys Ser Ser Gln Pro Ser Gln Thr Glu Leu Val 
        835                 840                 845             


Arg Tyr Lys Leu Trp Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Gly 
    850                 855                 860                 


Glu Ile Ile Pro Leu Gly Lys Leu Phe Thr Ser Ala Tyr Glu Ile Glu 
865                 870                 875                 880 


His Val Ile Pro Gln Ser Arg Tyr Phe Asp Asp Ser Leu Ser Asn Lys 
                885                 890                 895     


Val Ile Cys Glu Ser Glu Val Asn Lys Leu Lys Gly Asn Leu Leu Gly 
            900                 905                 910         


Tyr Glu Phe Val Lys Asn His His Gly Val Lys Val Glu Leu Asn Phe 
        915                 920                 925             


Asn Lys Thr Val Glu Ile Phe Ser Ile Asn Glu Tyr Glu Glu Phe Val 
    930                 935                 940                 


Lys Gly Asn Tyr Gly Lys Asn Arg Gly Lys Met Lys Lys Leu Leu Leu 
945                 950                 955                 960 


Glu Asp Ile Pro Lys Ser Phe Ile Glu Arg Gln Leu Asn Asp Thr Arg 
                965                 970                 975     


Tyr Ile Ser Lys Val Val Lys Gly Leu Leu Ser Asn Ile Val Arg Glu 
            980                 985                 990         


Asp Gly Glu Gln Glu Ala Thr Ser  Lys His Val Ile Pro  Cys Thr Gly 
        995                 1000                 1005             


Gly Val  Thr Thr Ile Leu Lys  Gln Asp Trp Gly Leu  Asn Asp Val 
    1010                 1015                 1020             


Trp Asn  Lys Ile Val Thr Pro  Arg Phe Glu Arg Met  Asn Glu Leu 
    1025                 1030                 1035             


Thr Asn  Thr Ser Leu Phe Gly  Gln Trp Glu Asn Lys  Glu Gly Lys 
    1040                 1045                 1050             


Arg Ile  Phe Arg Thr Glu Val  Pro Leu Glu Leu Gln  Lys Gly Phe 
    1055                 1060                 1065             


Asn Lys  Lys Arg Ile Asp His  Arg His His Ala Met  Asp Ala Ile 
    1070                 1075                 1080             


Val Ile  Ala Cys Ala Thr Arg  Ser His Val Thr Tyr  Leu Asn Asn 
    1085                 1090                 1095             


Glu Ser  Ser Lys Ala Asn Ala  Lys Glu Ile Arg Asp  Gly Leu Lys 
    1100                 1105                 1110             


His Lys  Leu Cys Phe Lys Ser  Lys Thr Asp Glu Thr  Gly Asn Tyr 
    1115                 1120                 1125             


Lys Trp  Gln Phe His Lys Pro  Trp Asp Thr Phe Thr  Gln Asp Val 
    1130                 1135                 1140             


Gln Asp  Thr Leu Glu Asn Ile  Val Ile Ser Phe Lys  Gln Asn Leu 
    1145                 1150                 1155             


Arg Val  Ile Asn Lys Thr Thr  Asn Arg Tyr Gln Lys  Tyr Glu Glu 
    1160                 1165                 1170             


Glu Lys  Lys Ile Leu Lys Ser  Gln Glu Lys Gly Asp  Ser Trp Ala 
    1175                 1180                 1185             


Ile Arg  Lys Pro Leu His Lys  Asp Thr Val Phe Ala  Lys Val Ser 
    1190                 1195                 1200             


Leu Gln  Lys Ile Lys Cys Val  Lys Leu Ala Met Ala  Leu Lys Asp 
    1205                 1210                 1215             


Trp Lys  Asn Ile Val Asp Lys  Gln Leu Lys Lys Glu  Ile Arg Arg 
    1220                 1225                 1230             


Leu Ile  Leu Ser Tyr Gly Lys  Phe Asp Ala Glu Ile  Leu Asn Lys 
    1235                 1240                 1245             


Tyr Phe  Lys Asp Arg Lys Tyr  Ile Phe Lys Asp Val  Asp Ile Ser 
    1250                 1255                 1260             


Lys Val  Asp Val Tyr Tyr Phe  Glu Thr Glu Tyr Val  Ala Thr Arg 
    1265                 1270                 1275             


Lys Asn  Leu Asp Thr Ser Phe  Asp Arg Lys Ser Ile  Glu Ser Ile 
    1280                 1285                 1290             


Thr Asp  Thr Gly Ile Gln Lys  Ile Leu Leu Asn His  Leu Gly Val 
    1295                 1300                 1305             


Lys Asn  Asn Asn Pro Glu Leu  Ala Phe Ser Ala Glu  Gly Ile Asp 
    1310                 1315                 1320             


Glu Met  Asn Gln Arg Ile Val  Glu Leu Asn Gly Gly  Lys Gln His 
    1325                 1330                 1335             


Gln Pro  Ile Tyr Lys Val Arg  Met Tyr Glu Pro Leu  Gly Asn Lys 
    1340                 1345                 1350             


Phe Gln  Val Gly Arg Ile Gly  Asn Lys Lys Asp Lys  Tyr Val Glu 
    1355                 1360                 1365             


Ala Ala  Pro Gly Thr Asn Leu  Phe Tyr Ala Ile Tyr  Gln Ser Glu 
    1370                 1375                 1380             


Asp Lys  Lys Arg Ile Tyr Asp  Thr Ile Pro Leu Asn  Ile Ile Ile 
    1385                 1390                 1395             


Glu Arg  Gln Lys Lys Glu Leu  Phe Pro Val Pro Glu  Asn Lys Glu 
    1400                 1405                 1410             


Asp Lys  Asp Asn Leu Tyr Lys  Leu Leu Phe Trp Leu  Ser Pro Asn 
    1415                 1420                 1425             


Asp Met  Val Tyr Leu Pro Thr  Glu Lys Glu Trp Ala  Asn Pro Asp 
    1430                 1435                 1440             


Leu Phe  Asn Phe Asn Asn Met  Thr Lys Asn Gln Arg  Lys Lys Leu 
    1445                 1450                 1455             


Tyr Lys  Phe Ile Asp Gly Ser  Gly Thr Thr Ala Asn  Phe Ile Pro 
    1460                 1465                 1470             


Asn Thr  Val Ala Asn Ile Ile  Phe Ser Ile Pro Lys  Lys Asp Ser 
    1475                 1480                 1485             


Gly Lys  Tyr Ser Met Gly Asn  Arg Ile Val Gln Asn  Glu Phe Gly 
    1490                 1495                 1500             


Ile Gly  Ser Pro Gln Ser Lys  Asn Gln Lys Ala Phe  Thr Gly Glu 
    1505                 1510                 1515             


Met Ile  Lys Glu Arg Cys Trp  Lys Leu Lys Ile Asn  Arg Leu Gly 
    1520                 1525                 1530             


Asp Ile  Ile Glu Cys Ile Lys  
    1535                 1540 


<210>  1340
<211>  1542
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1340

Met Glu Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      


Trp Ala Leu Val Lys Gly Glu Arg His Glu Asn Asp Asn Glu Thr Ile 
            20                  25                  30          


Leu Ser Tyr Ile Glu Ala Ala Gly Ser Arg Ile Leu Pro Leu Asp Ala 
        35                  40                  45              


Ala Ala Gln Gly Asp Phe Glu Lys Gly Asn Pro Ala Lys Thr Gln Thr 
    50                  55                  60                  


Ser Glu Arg Thr Arg Met Arg Gly Met Arg His Leu Leu Glu Arg Ser 
65                  70                  75                  80  


Leu Leu Arg Arg Glu Arg Leu His Arg Val Leu Asp Ile Met Asp Phe 
                85                  90                  95      


Leu Pro Pro His Tyr Ser Gln Ser Leu Thr Arg Tyr Gly Lys Phe Lys 
            100                 105                 110         


Ala Asp Thr Glu Cys Lys Leu Ala Trp Arg Lys Thr Glu Thr Gly His 
        115                 120                 125             


Tyr Glu Phe Leu Phe Gln Asp Ser Phe Gln Glu Met Leu Gln Asp Phe 
    130                 135                 140                 


Gln Asn His Gly Lys Leu Glu Glu Asn Lys Lys Ile Pro Tyr Asp Trp 
145                 150                 155                 160 


Thr Ile Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Pro Val Ser Lys 
                165                 170                 175     


Gln Glu Leu Ala Trp Ile Leu Leu Asn Phe Asn Gln Lys Arg Gly Tyr 
            180                 185                 190         


Tyr Gln Arg Glu Asp Gln Ala Val Asp Asp Asn Gln Lys Lys Arg Val 
        195                 200                 205             


Glu Tyr Gln Ala Leu Lys Val Ile Ser Val Glu Asp Thr Gly Glu Lys 
    210                 215                 220                 


Lys Gly Lys Asn Thr Trp Tyr Asp Val Arg Leu Glu Asn Gly Leu Val 
225                 230                 235                 240 


Tyr His Tyr Ser Ala Pro Ile Lys Pro Asp Trp Lys Gly Glu Thr Lys 
                245                 250                 255     


Glu Phe Ile Val Thr Thr Thr Ile Asp Gln Asn Gly Lys Pro Glu Leu 
            260                 265                 270         


Asp Lys Asp Gly Lys Pro Lys Tyr Ser Met Arg Ala Pro Lys Glu Asp 
        275                 280                 285             


Asp Tyr Lys Leu Leu Met Glu Lys Thr Lys Lys Asp Ile Thr Asp Ser 
    290                 295                 300                 


Gly Lys Ser Val Gly Glu Tyr Ile Tyr Asp Ala Leu Leu Val Asn Pro 
305                 310                 315                 320 


Gln Gln Lys Ile Arg Gly Gly Leu Val Arg Thr Ile Glu Arg Tyr Phe 
                325                 330                 335     


Tyr Lys Lys Glu Leu Glu Lys Ile Leu Leu Ser Gln Glu Arg Phe Ile 
            340                 345                 350         


Pro Glu Leu Thr Asp Arg Glu Leu Tyr Arg Arg Cys Val Glu Glu Leu 
        355                 360                 365             


Tyr Pro Ser Asn Asp Ala Tyr Arg His Leu Ile Glu Ser Arg Asp Met 
    370                 375                 380                 


Arg Tyr Leu Leu Val Glu Asp Ile Leu Phe Tyr Gln Arg Pro Leu Lys 
385                 390                 395                 400 


Ser Lys Lys Ser Leu Ile Asp Asp Cys Pro Tyr Glu Ser Arg Ser Tyr 
                405                 410                 415     


Val Asp Arg Glu Thr Gly Glu Ile Lys Ser Ser Pro Val Lys Cys Ile 
            420                 425                 430         


Ala Gln Ser His Pro Leu Phe Gln Glu Phe Arg Leu Trp Gln Phe Ile 
        435                 440                 445             


His Asn Leu Arg Ile Tyr Gln Asn Glu Arg Ser Val Glu Gly Lys Leu 
    450                 455                 460                 


Gln Leu Asp Val Asp Val Thr Glu Glu Val Leu Pro Thr Pro Glu Ser 
465                 470                 475                 480 


Lys Ala Ala Leu Phe Arg Phe Leu Asn Asp Arg Glu Asn Val Thr Gln 
                485                 490                 495     


Glu Ile Leu Leu Lys Glu Val Phe Lys Gly Arg Lys Leu Ser Val Lys 
            500                 505                 510         


Asp Cys Pro Tyr Arg Trp Asn Tyr Ala Gly Glu Gly Lys Lys Tyr Pro 
        515                 520                 525             


Cys Asn Glu Thr Arg His Leu Met Leu Lys Leu Leu Lys Lys Ala Gly 
    530                 535                 540                 


Ile Asp Thr Ala Phe Leu Thr Pro Glu Lys Glu Gln Ala Leu Trp His 
545                 550                 555                 560 


Ile Met Tyr Ser Val Glu Asp Lys Asp Gln Tyr Glu Lys Ala Leu Ser 
                565                 570                 575     


Thr Phe Ala Arg Lys Asn Asp Leu Pro Asp Gly Phe Lys Glu Gln Phe 
            580                 585                 590         


Lys Lys Ala Pro Thr Phe Lys Lys Asp Tyr Gly Ala Tyr Ser Leu Arg 
        595                 600                 605             


Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Val Gly Glu Tyr Trp Ser 
    610                 615                 620                 


Ala Asp Cys Ile Asp Ser Glu Thr Lys Glu Arg Ile Ser Gln Ile Ile 
625                 630                 635                 640 


Arg Gly Glu Met Asp Glu Lys Val Ser Gln Arg Met Thr Lys Ser Arg 
                645                 650                 655     


Asp Ser Phe Thr Asp Ile Ser Gln Phe Gln Gly Leu Pro Val Trp Gln 
            660                 665                 670         


Ala Cys Tyr Val Val Tyr Gly Arg His Ser Glu Ala Thr Glu Ile Gln 
        675                 680                 685             


Arg Trp Glu Lys Pro Glu Asp Ile Asp Val Tyr Leu Lys Ala Phe Lys 
    690                 695                 700                 


Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val Leu Glu Thr 
705                 710                 715                 720 


Leu Arg Val Val Arg Asp Ile Trp Lys Thr Tyr Gly Lys Val Asp Glu 
                725                 730                 735     


Ile His Ile Glu Met Gly Arg Asp Met Lys Asn Thr Ala Glu Glu Arg 
            740                 745                 750         


Lys Arg Ile Phe Asn Arg Asn Gln Gln Asn Glu Asn Asp Asn Leu Arg 
        755                 760                 765             


Ile Lys Ala Leu Leu Thr Glu Phe Met Asn Pro Asp Phe Glu Ile Glu 
    770                 775                 780                 


Gly Val Arg Pro Tyr Ser Pro Ser Gln Gln Glu Ala Leu Arg Ile Tyr 
785                 790                 795                 800 


Glu Asp Gly Ala Trp Ser Gly Leu Asp Glu Glu Lys Glu Asp Lys Lys 
                805                 810                 815     


Glu Leu Glu Ala Ile Arg Glu Ile Leu Gly Lys Phe Ser Gln Ser Asp 
            820                 825                 830         


Lys Ala Lys Arg Pro Thr His Ser Glu Val Leu Arg Tyr Lys Ala Trp 
        835                 840                 845             


Leu Glu Gln Arg Tyr Ser Ser Pro Tyr Thr Gly Glu Ala Ile Pro Leu 
    850                 855                 860                 


Ala Lys Leu Phe Thr Pro Ala Tyr Glu Ile Glu His Val Ile Pro Gln 
865                 870                 875                 880 


Ser Arg Phe Phe Asp Asp Ser Phe Ser Asn Lys Val Val Cys Glu Ala 
                885                 890                 895     


Glu Val Asn Lys Leu Lys Asp Arg Met Leu Gly Tyr Glu Phe Ile Lys 
            900                 905                 910         


Ala His Gln Gly Glu Val Val Ser Leu Ser Gly Thr Gly Arg Cys Ala 
        915                 920                 925             


Arg Ile Leu Ser Pro Glu Glu Tyr Glu Gln Arg Val Thr Asp Cys Tyr 
    930                 935                 940                 


Lys His Asn Thr Ala Lys Met Lys Lys Leu Leu Met Glu Glu Ile Pro 
945                 950                 955                 960 


Asp Asp Phe Ile Gln Arg Gln Met Asn Asp Ser Arg Tyr Ile Ser Lys 
                965                 970                 975     


Leu Val Lys Ser Leu Leu Ser Asn Met Val Arg Glu Glu Gly Glu Arg 
            980                 985                 990         


Glu Ala Ile Ser Lys His Val Ile  Thr Cys Asn Gly Thr  Ile Thr Asp 
        995                 1000                 1005             


Arg Leu  Lys Lys Asp Trp Gly  Val Asn Asp Val Trp  Asn His Ile 
    1010                 1015                 1020             


Ile Leu  Pro Arg Phe Gln Arg  Met Lys Asp Leu Thr  Gly Arg Asn 
    1025                 1030                 1035             


Asp Phe  Val Ala Val Asn Ser  Gln Gly His Glu Ile  Pro Ser Val 
    1040                 1045                 1050             


Pro Pro  Glu Leu Gln Lys Gly  Phe Lys Lys Lys Arg  Ile Asp His 
    1055                 1060                 1065             


Arg His  His Ala Met Asp Ala  Ile Val Ile Ala Cys  Thr Thr Arg 
    1070                 1075                 1080             


Asn His  Val Asn Leu Leu Asn  Asn Glu Ala Ala Met  Ser Glu Asn 
    1085                 1090                 1095             


Lys Ala  Asn Arg Ile Ala Leu  Ser Arg Lys Leu Arg  Arg Tyr Glu 
    1100                 1105                 1110             


Asn Cys  Ala Ile Tyr Lys Gly  Gly Glu Thr Lys Asn  Ile Ser Val 
    1115                 1120                 1125             


Ala Lys  Glu Phe Leu Lys Pro  Trp Ala Thr Phe Pro  Ala Asp Val 
    1130                 1135                 1140             


Glu Arg  Ala Leu Gly Asp Ile  Val Val Ser Phe Lys  Gln Asn Leu 
    1145                 1150                 1155             


Arg Val  Ile Asn Lys Ala Thr  Asn His Tyr Glu His  Tyr Lys Asp 
    1160                 1165                 1170             


Gly Lys  Lys Val Lys Val Pro  Gln Lys Lys Gly Asp  Met Trp Val 
    1175                 1180                 1185             


Val Arg  Lys Pro Leu His Lys  Asp Thr Val Tyr Ala  Leu Val Asn 
    1190                 1195                 1200             


Leu Arg  Asn Val Lys Thr Val  Gly Leu Lys Asp Ala  Ile Leu Met 
    1205                 1210                 1215             


Pro Asp  Arg Ile Ala Asn Lys  Asp Leu Lys Lys Lys  Val Lys Glu 
    1220                 1225                 1230             


Leu Leu  Ala Gln Gly Asp Asp  Leu Lys Lys Ile Lys  Lys Tyr Phe 
    1235                 1240                 1245             


Glu Thr  Glu Lys Asp Thr Trp  Gln Asp Val Asp Val  Lys Lys Ile 
    1250                 1255                 1260             


Glu Val  Tyr Tyr Phe Ser Glu  Glu Thr Ala Asn His  Tyr Tyr Ala 
    1265                 1270                 1275             


Thr Arg  Lys Pro Phe Asp Ala  Asn Val Lys Ile Asp  Ser Val Thr 
    1280                 1285                 1290             


Asp Thr  Gly Ile Gln Lys Ile  Leu Arg Arg His Leu  Glu Glu Asn 
    1295                 1300                 1305             


Gly Asn  Lys Ala Asp Val Ala  Phe Ser Pro Glu Gly  Ile Ala Arg 
    1310                 1315                 1320             


Met Asn  Glu Asn Ile Phe Arg  Leu Asn Gly Asn Lys  His His Gln 
    1325                 1330                 1335             


Pro Ile  Tyr Lys Val Arg Val  Tyr Glu Lys Ala Asn  Lys Tyr Pro 
    1340                 1345                 1350             


Val Gly  Gln Thr Gly Asn Lys  Ala Ser Lys Phe Val  Glu Ala Ala 
    1355                 1360                 1365             


Lys Gly  Thr Asn Leu Tyr Leu  Ala Val Tyr Glu Ser  Glu Val Val 
    1370                 1375                 1380             


Asp Lys  Lys Thr Ala Glu Ile  Ser Lys Lys Arg Ser  Tyr Val Ile 
    1385                 1390                 1395             


Val Pro  Leu Arg Asp Ala Val  Ala Arg Gln Lys Gln  Gly Val Pro 
    1400                 1405                 1410             


Val Ala  Pro Glu Arg Asn Glu  Glu Gly Gly Lys Leu  Leu Phe Val 
    1415                 1420                 1425             


Leu Ser  Pro Lys Asp Phe Val  Tyr Val Pro Thr Glu  Glu Glu Arg 
    1430                 1435                 1440             


Ser Lys  Gly Glu Val Leu Leu  Pro Leu Asp Lys Asn  Arg Ile Tyr 
    1445                 1450                 1455             


Ile Met  Met Asp Ala Ser Asp  Gly Ile Val Asn Phe  Leu Pro Tyr 
    1460                 1465                 1470             


Ser Val  Ala Asn Leu Ile Tyr  Ala Leu Pro Lys Asp  Gln Ala Ser 
    1475                 1480                 1485             


Gly Phe  Cys Thr Asp Gly Arg  Met Ile Gln Asn Glu  Phe Gly Leu 
    1490                 1495                 1500             


Gly Ser  Pro Lys Ser Lys Asn  Glu Arg Ala Leu Thr  Gly Glu Met 
    1505                 1510                 1515             


Ile Lys  Glu Ile Cys Leu Pro  Val Lys Val Asp Arg  Leu Gly Arg 
    1520                 1525                 1530             


Ile Thr  Glu Ile Asn Gly Asn  Lys Val 
    1535                 1540         


<210>  1341
<211>  1547
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1341

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Thr Asn Asn Asp Phe Asp Asn Lys Leu Gly Ser Ile Glu Gly 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Ser Gln Asp Val Leu Gly Lys Phe 
        35                  40                  45              


Asp Ser Gly Val Ser Ile Ser Gln Thr Ala Glu Arg Thr Lys Tyr Arg 
    50                  55                  60                  


Gly Thr Arg Arg Leu Val Gln Arg Phe Leu Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Ile Leu Asn Ile Leu Asp Phe Leu Pro Arg His Tyr Ser Glu 
                85                  90                  95      


Ala Ile Asp Phe Asp Lys Lys Phe Gly Gln Phe Lys Pro Glu Arg Glu 
            100                 105                 110         


Glu Lys Leu Ser Phe Tyr Lys Asn Glu Ser Gly Lys Asn Gln Phe Leu 
        115                 120                 125             


Phe Leu Glu Ser His Gln Glu Met Phe Ser Glu Phe Lys Leu Thr His 
    130                 135                 140                 


Pro Asp Leu Lys Gln Val Pro Tyr Asp Trp Thr Ile Tyr Tyr Leu Arg 
145                 150                 155                 160 


Lys Lys Ala Leu Thr Gln Lys Ile Ser Lys Glu Glu Leu Ala Trp Ile 
                165                 170                 175     


Ile Leu Asn Phe Asn Gln Lys Arg Gly Tyr Tyr Gln Leu Arg Gly Glu 
            180                 185                 190         


Glu Glu Glu Val Asn Lys Asn Lys Leu Glu Glu Phe His Thr Leu Val 
        195                 200                 205             


Ile Val Asp Val Ile Glu Arg Glu Lys Gly Lys Ser Gly Val Trp Tyr 
    210                 215                 220                 


Asn Val Ile Leu Glu Asn Gly Trp Ile Tyr Lys Arg Glu Ser Lys Ile 
225                 230                 235                 240 


Ala Leu Phe Asp Trp Ile Gly Lys Gln Lys Asn Phe Ile Val Thr Thr 
                245                 250                 255     


Glu Leu Asn Asp Asp Gly Thr Ile Lys Leu Asn Lys Asp Asn Glu Glu 
            260                 265                 270         


Lys Arg Ser Phe Arg Ser Pro Lys Glu Asp Asp Trp Gly Leu Arg Lys 
        275                 280                 285             


Ile Arg Thr Glu Gln Thr Ile Asn Gln Ser Glu Lys Ser Val Gly Ser 
    290                 295                 300                 


Phe Ile Tyr Asp Glu Leu Leu Lys Lys Pro Asn Gln Lys Ile Lys Gly 
305                 310                 315                 320 


Glu Leu Val Arg Thr Ile Glu Arg Lys Phe Tyr Lys Asp Glu Leu Ser 
                325                 330                 335     


Lys Ile Leu Lys Ala Gln Ile Thr Phe His Tyr Glu Ile Leu Gly Asp 
            340                 345                 350         


Arg Asn Ile Tyr Lys Lys Cys Ile Asp Glu Leu Tyr Arg His Asn Asp 
        355                 360                 365             


Ser His Lys Asn Asn Ile Lys Asp Lys Gly Phe Asp Tyr Leu Phe Leu 
    370                 375                 380                 


Asp Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys Thr Ser Leu 
385                 390                 395                 400 


Ile Ser Asp Cys Ser Leu Glu Phe Val Pro Ile Lys Asp Lys Lys Gly 
                405                 410                 415     


Ile Leu Val Lys Asp Ser Asn Gly Lys Gln Val Val Lys Pro Leu Lys 
            420                 425                 430         


Cys Ile Ala Lys Ser Asn Pro Ile Phe Gln Glu Phe Arg Leu Leu Gln 
        435                 440                 445             


Phe Val Lys Asn Leu Lys Ile Tyr Lys Lys Glu Ile Ile Asn Glu Ile 
    450                 455                 460                 


Asp Val Thr Asn Asp Tyr Leu Lys Thr Glu Glu Asp Leu Val Glu Leu 
465                 470                 475                 480 


Phe Asp Trp Leu Asn Asp Lys Ala Glu Ile Ser Gln Lys Gln Phe Leu 
                485                 490                 495     


Ala Tyr Pro Lys Phe Lys Leu Lys Glu Asp Lys Tyr Arg Trp Asn Phe 
            500                 505                 510         


Val Glu Asp Lys Ile Tyr Pro Cys Asn Glu Thr Arg Asn Gln Phe Leu 
        515                 520                 525             


Asn Ala Ile Ala Lys Ile Asp Val Asn Lys Ser Phe Phe Asp Ala Lys 
    530                 535                 540                 


Asn Thr Gln Asp Leu Trp His Ile Leu Tyr Ser Val Thr Asp Lys Thr 
545                 550                 555                 560 


Glu Leu Phe Ser Ile Asn Thr Glu Thr Gly Ile Gln Ser Gly Ala Leu 
                565                 570                 575     


Ser Thr Phe Ala Lys Arg His Asn Leu Ser Asn Glu Phe Val Ser Asn 
            580                 585                 590         


Phe Ser Lys Ile Lys Pro Tyr Asn Lys Asp Tyr Gly Ser Tyr Ser Glu 
        595                 600                 605             


Lys Ala Ile Lys Lys Leu Leu Pro Leu Met Arg Phe Gly Ser Asn Trp 
    610                 615                 620                 


Asn Lys Asn Asn Ile Asp Ala Lys Thr Leu Ala Arg Ile Asp Lys Leu 
625                 630                 635                 640 


Gln Thr Gly Glu Phe Asp Glu Thr Met Arg Thr Arg Val Arg Glu Lys 
                645                 650                 655     


Ala Ile His Leu Ser Lys Ile Thr Asp Phe Gln Gly Leu Pro Leu Trp 
            660                 665                 670         


Leu Ala Ser Tyr Ile Ile Tyr Asp Arg His Ser Glu Ala Ser Asn Ile 
        675                 680                 685             


Ser Lys Trp Lys Thr Pro Glu Asp Ile Ala Trp Phe Leu Asn Pro Lys 
    690                 695                 700                 


Asn Lys Ser Gly Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu 
705                 710                 715                 720 


Gln Val Ile Thr Glu Thr Leu Arg Val Val Lys Asp Ile Trp Gln His 
                725                 730                 735     


Tyr Gly Glu Gly Lys Glu Asn Phe Phe Asn Glu Ile His Ile Glu Leu 
            740                 745                 750         


Gly Arg Glu Met Lys Asn Asn Ala Asn Asp Arg Lys Arg Ile Thr Glu 
        755                 760                 765             


Thr Ile Asn Gln Asn Glu Asn Thr Asn Leu Arg Ile Lys Ala Ile Leu 
    770                 775                 780                 


Thr Glu Leu Lys Asn Asp Gly Ile Asn Asp Val Arg Pro Tyr Ser Pro 
785                 790                 795                 800 


Ser Gln Gln Glu Ile Leu Lys Ile Tyr Glu Glu Gly Val Tyr Asn Ser 
                805                 810                 815     


Glu Ile Arg Lys Glu Lys Leu Glu Asp Ile Asp Lys Ile Arg Lys Asn 
            820                 825                 830         


Ala Lys Pro Thr Pro Ser Glu Ile Ile Lys Tyr Lys Leu Trp Leu Glu 
        835                 840                 845             


Gln Gly Tyr Ile Ser Pro Tyr Thr Gly Lys Leu Ile Pro Leu Ser Asp 
    850                 855                 860                 


Leu Phe Thr Thr Lys Tyr Gln Ile Glu His Ile Ile Pro Gln Ser Arg 
865                 870                 875                 880 


Leu Phe Asp Asp Ser Leu Ser Asn Lys Val Ile Cys Glu Ser Glu Val 
                885                 890                 895     


Asn Asp Leu Lys Gly Asn Asn Thr Ala Leu Glu Phe Ile Leu Lys Asn 
            900                 905                 910         


Glu Gly Arg Ile Val Leu Leu Thr Gly Gly Lys Thr Val Lys Ile Phe 
        915                 920                 925             


Thr Lys Glu Ala Tyr Asn Gln His Ile Thr Ser Tyr Tyr Ile Lys Asn 
    930                 935                 940                 


Lys Ser Lys Gln Lys Arg Leu Leu Thr Glu Glu Ile Pro Glu Lys Phe 
945                 950                 955                 960 


Ile Asp Arg Gln Leu Asn Asp Thr Lys Tyr Ile Ser Lys Ile Val Lys 
                965                 970                 975     


Asn Leu Leu Ser Lys Ile Val Arg Glu Glu Asp Glu Gln Glu Val Thr 
            980                 985                 990         


Ser Lys His Ile Val Ser Leu Asn  Gly Ser Ile Thr Ser  Arg Met Lys 
        995                 1000                 1005             


Gln Asp  Trp Gly Leu Asn Asp  Val Trp Asn Lys Ile  Val Ser Pro 
    1010                 1015                 1020             


Arg Phe  Glu Arg Leu Asn Ala  Ile Thr Asn Ser Asn  Asp Phe Gly 
    1025                 1030                 1035             


Lys Leu  Glu Leu Lys Lys Asp  Glu Phe Gly Asn Ala  Gly Lys Gln 
    1040                 1045                 1050             


Val Phe  Gln Thr Thr Val Pro  Asp Asp Ile Ala Lys  Gly Phe Ser 
    1055                 1060                 1065             


Lys Lys  Arg Ile Asp His Arg  His His Ala Leu Asp  Ala Leu Val 
    1070                 1075                 1080             


Ile Ala  Cys Ile Asn Arg Thr  His Ile Asn Tyr Leu  Asn Asn Leu 
    1085                 1090                 1095             


Asn Ala  Lys Asp Glu Asn Asp  Lys Thr Ile Lys His  Glu Leu Arg 
    1100                 1105                 1110             


Asn Lys  Leu Cys Phe Lys Thr  Lys Pro Asp Ser Asn  Gly Asn Ser 
    1115                 1120                 1125             


Lys Trp  Glu Phe His Lys Pro  Trp Glu Asn Phe Thr  Lys Lys Val 
    1130                 1135                 1140             


Glu Asp  Glu Leu Asn Gln Thr  Ile Ile Ser Phe Lys  Gln Asn Thr 
    1145                 1150                 1155             


Arg Val  Ile Asn Lys Thr Val  Asn Lys Tyr Gln Val  Trp Lys Glu 
    1160                 1165                 1170             


Glu Asp  Gly Ala Met Lys Lys  Val Thr Val Lys Gln  Thr Lys Gly 
    1175                 1180                 1185             


Asp Asn  Trp Ala Ile Arg Lys  Pro Ile His Ala Glu  Thr Val Ser 
    1190                 1195                 1200             


Gly Lys  Val Phe Leu Lys Arg  Ile Lys Gln Ser His  Ile Thr Ile 
    1205                 1210                 1215             


Ala Asn  Ala Ile Glu Gln Ile  Glu Leu Ile Val Asp  Lys Glu Val 
    1220                 1225                 1230             


Arg Lys  Gln Leu Lys Ala Lys  Ile Lys Gln Tyr Pro  Asn Asn Lys 
    1235                 1240                 1245             


Glu Gly  Phe Lys Lys His Leu  Lys Ala Phe Pro Ile  Met Ile Asn 
    1250                 1255                 1260             


Gly Lys  Ala Ile Asp Lys Val  Gln Ile Tyr Glu Thr  Ile Glu Ala 
    1265                 1270                 1275             


Thr Ala  Thr Arg Glu Arg Asn  Asn Leu Asp Ile Thr  Phe Asp Glu 
    1280                 1285                 1290             


Lys Lys  Ile Glu Lys Ile Thr  Asp Thr Gly Ile Gln  Lys Ile Leu 
    1295                 1300                 1305             


Leu Asn  His Leu Lys Gln Glu  Ile Tyr Gln Asn Ala  Ile Asp Glu 
    1310                 1315                 1320             


Ser Gly  Lys Lys Ile Ala Pro  His Glu Val Ala Phe  Ser Glu Ile 
    1325                 1330                 1335             


Gly Ile  Asp Glu Leu Asn Lys  Asn Ile Lys Val Leu  Asn Asn Gly 
    1340                 1345                 1350             


Lys Asn  His Gln Pro Ile Lys  Lys Val Arg Ile Phe  Glu Glu Ser 
    1355                 1360                 1365             


Lys Arg  Phe Ser Leu Gly Gln  Ser Gly Ser Lys Thr  Asp Lys Tyr 
    1370                 1375                 1380             


Val Glu  Thr Ala Lys Gly Thr  Asn Leu Phe Tyr Ala  Ile Tyr Glu 
    1385                 1390                 1395             


Gly Lys  Lys Gly Asn Arg Asn  Tyr Lys Thr Ile Pro  Leu Asn Glu 
    1400                 1405                 1410             


Val Ile  Glu Arg Gln Lys Gln  Gly Leu Asn Ser Ala  Leu Val Thr 
    1415                 1420                 1425             


Asp Glu  Asn Glu Asn Arg Leu  Leu Phe Thr Leu Ser  Pro Asn Asp 
    1430                 1435                 1440             


Leu Val  Tyr Ile Pro Thr Asn  Glu Glu Lys Lys Asn  Leu Thr Thr 
    1445                 1450                 1455             


Ile Asn  Phe Asp Lys Leu Ser  Arg Glu Gln Thr Glu  Arg Ile Tyr 
    1460                 1465                 1470             


Lys Phe  Val Ser Cys Thr Ser  Gly Glu Gly His Phe  Val Ser Asn 
    1475                 1480                 1485             


Asn Tyr  Ser Lys Glu Ile Ile  Ser Asn Glu Asn Gly  Ser Asn Asn 
    1490                 1495                 1500             


Lys Asn  Glu Arg Met Leu Glu  Phe Asn Asn Ser Asn  Thr Ile Tyr 
    1505                 1510                 1515             


Asp Glu  Lys Glu Lys Pro Val  Met Ile Lys Ser Val  Cys Trp Lys 
    1520                 1525                 1530             


Leu Glu  Val Asp Arg Leu Gly  Lys Ile Lys Arg Ile  Ile Arg 
    1535                 1540                 1545         


<210>  1342
<211>  1558
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1342

Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Val Asn Val Asp Lys Asn Gly Asn Pro Phe Gly Ile Glu Gly 
            20                  25                  30          


Leu Gly Ser Arg Ile Ile Pro Met Asp Gln Ala Thr Leu Ser Asp Phe 
        35                  40                  45              


Glu Lys Gly Asn Ser Lys Ser Gln Thr Ala Glu Arg Thr Met Phe Arg 
    50                  55                  60                  


Gly Ala Arg Arg Leu Arg Glu Arg Lys His Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  


His Arg Ile Leu Asn Glu Leu Asn Phe Leu Pro Ile His Tyr Arg Ser 
                85                  90                  95      


Cys Ile Asp Phe Val His Arg Leu Gly Lys Phe Lys Ala Asn His Glu 
            100                 105                 110         


Pro Lys Ile Ala Trp Arg Ser Asn Asp Gln Asp Lys Met Glu Phe Ile 
        115                 120                 125             


Phe Gln Asn Ser Phe Leu Glu Met Met Ser Asp Phe Thr Lys Arg Gln 
    130                 135                 140                 


Pro Glu Leu Val Lys Glu Gly Lys Lys Ile Pro Tyr Asp Trp Thr Ile 
145                 150                 155                 160 


Tyr Phe Leu Arg Lys Lys Ala Leu Gly Gln Arg Ile Glu Lys Glu Glu 
                165                 170                 175     


Leu Ala Trp Leu Leu Leu His Phe Asn Gln Lys Arg Gly Tyr Tyr Gln 
            180                 185                 190         


Leu Arg Gly Glu Asp Glu Ile Asp Ala Thr Arg Ser Ala Lys Thr Arg 
        195                 200                 205             


Lys Tyr Phe Asp Arg Lys Ile Ile Lys Ser Ile Ile Asp Thr Gly Glu 
    210                 215                 220                 


Glu Tyr Lys Gly Leu Lys Val Leu Ile Val Glu Leu Asp Asn Gly Asn 
225                 230                 235                 240 


Gln Gly Lys Ile Phe Lys Gln Glu Val Pro Asp Trp Val Gly Gln Glu 
                245                 250                 255     


Lys Asn Ile Ile Ala Thr Val Asp Leu Asp Lys Asp Gly Lys Asp Arg 
            260                 265                 270         


Phe Asp Lys Gly Val Leu Ser Gln Arg Phe Ser Ile Pro Ser Asp Ser 
        275                 280                 285             


Glu Trp Glu Thr Glu Trp Lys Leu Ile Lys Leu Lys Thr Glu Glu Asp 
    290                 295                 300                 


Leu Glu Ala Ser Gln Lys Thr Val Gly Thr Tyr Ile Tyr Asp Ser Leu 
305                 310                 315                 320 


Leu Lys Asn Pro Asn Gln Lys Ile Lys Gly Lys Leu Val Arg Thr Ile 
                325                 330                 335     


Glu Arg Lys Tyr Tyr Arg Asp Glu Leu Arg Ala Ile Leu Asn Lys Gln 
            340                 345                 350         


Arg Glu Phe His Pro Glu Leu Asn Asp Lys Lys Leu Tyr Gln His Cys 
        355                 360                 365             


Ile Phe Ser Leu Tyr Pro Gln Asn Glu Ala Phe Arg Asn Ser Ile Ala 
    370                 375                 380                 


Asn Lys Asp Phe Thr Tyr Leu Phe Leu Glu Asp Ile Ile Phe Tyr Gln 
385                 390                 395                 400 


Arg Pro Leu Lys Ser Lys Lys Ser Thr Ile Asn Asn Cys Ala Tyr Glu 
                405                 410                 415     


Val Arg Arg Tyr Met Asp Lys Glu Thr Lys Glu Leu Lys Glu Val Pro 
            420                 425                 430         


Leu Lys Cys Ile Ala Lys Ser His Pro Ile Phe Gln Glu Phe Arg Leu 
        435                 440                 445             


Trp Gln Phe Ile Ser Asn Leu Arg Ile Tyr Lys Lys Asp Ile Thr Asp 
    450                 455                 460                 


Ile Asp Val Thr Asn Asp Phe Leu His Ser Gln Asp Asp Tyr Ala Asn 
465                 470                 475                 480 


Leu Phe Glu Trp Leu Asn Asn Lys Asn Glu Ile Asp Gln Lys Ala Phe 
                485                 490                 495     


Leu Lys Tyr Pro Ala Phe Gly Phe Lys Lys Asp Ala Val Asn Tyr Arg 
            500                 505                 510         


Trp Asn Tyr Val Glu Asp Lys Ser Tyr Pro Cys Asn Lys Thr Arg Ser 
        515                 520                 525             


Ala Ile Leu Ser Lys Leu Asp Lys His Glu Gln Glu Phe Leu Thr Lys 
    530                 535                 540                 


Glu Leu Glu Leu Lys Ile Trp Gln Leu Leu Tyr Ser Val Asn Ser Lys 
545                 550                 555                 560 


Glu Glu Ile Asp Lys Ala Leu Asn Val Asn Ser Pro Lys Ile Asn Asn 
                565                 570                 575     


Lys Ile Ser Ile Asp His Thr Gly Ile Leu His Glu Leu Lys Ser Ser 
            580                 585                 590         


Ser Leu Ser Asp Ile Ser Ile Gln Arg Leu Lys Ala Ile Asn Phe Glu 
        595                 600                 605             


Glu Lys Asp Tyr Gly Ser Tyr Ser Tyr Lys Ala Ile Lys Lys Leu Leu 
    610                 615                 620                 


Ala Leu Met Arg Gln Gly Lys Tyr Phe Asp Asn Thr Ala Ile Thr Asp 
625                 630                 635                 640 


Ala Thr Lys Ala Arg Ile Asn Ser Ile Phe Asp Arg Leu Glu Ser Ile 
                645                 650                 655     


Ser Phe Asp Pro Glu Arg Ile Asn Ser Ile Ser Asp Asp Asp Ile Pro 
            660                 665                 670         


Lys Gln Ile Leu Lys Ser Phe Ile Lys Leu Asn Arg Thr Tyr Gly Gly 
        675                 680                 685             


Phe Asn Thr Tyr Gln Ala Cys Tyr Ala Val Tyr Gly Arg His Ser Glu 
    690                 695                 700                 


Ala Lys Glu Ile Ala Lys Trp Glu Lys Pro Glu Asp Ile Asp Gln Tyr 
705                 710                 715                 720 


Leu Ala Lys Phe Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln 
                725                 730                 735     


Ile Ile Thr Glu Thr Leu Arg Val Val Arg Asp Ile Trp Lys Lys His 
            740                 745                 750         


Ala Tyr Lys Thr Ile Asn Lys Asp Asn Gln Gly Asn Asn Cys Glu Ser 
        755                 760                 765             


Glu Ser Glu Leu Thr Glu Ile His Ile Glu Leu Gly Arg Asp Met Lys 
    770                 775                 780                 


Asn Asn Lys Ser Glu Arg Leu Arg Ile Ser Arg Ile Asn Ser Glu Asn 
785                 790                 795                 800 


Glu Asn Thr Asn Leu Arg Ile Arg Lys Leu Leu Ile Glu Phe Asn Asp 
                805                 810                 815     


Pro Glu Phe Gly Val Glu Asn Val Arg Pro His Ser Pro Ser Gln Gln 
            820                 825                 830         


Glu Ile Leu Arg Ile Tyr Glu Asp Gly Val Leu Lys Ser Gly Val Glu 
        835                 840                 845             


Ile Pro Glu Tyr Val Asp Asn Ile Ile Lys Lys Phe Lys Glu Thr Asp 
    850                 855                 860                 


Val Lys Lys Gln Pro Ser Lys Ser Glu Val Leu Arg Tyr Lys Leu Trp 
865                 870                 875                 880 


Leu Glu Gln Lys Tyr Arg Ser Pro Tyr Thr Asn Glu Val Ile Pro Leu 
                885                 890                 895     


Gly Lys Leu Phe Thr Asn Asp Tyr Gln Ile Glu His Ile Ile Pro Gln 
            900                 905                 910         


Ser Arg Tyr Phe Asp Asp Ser Phe Ser Asn Lys Val Ile Cys Glu Ala 
        915                 920                 925             


Glu Val Asn Gln Leu Lys Glu Asn Glu Leu Gly Cys Glu Phe Ile Met 
    930                 935                 940                 


Asn Asn Gly Gly Arg Ile Val Glu Leu Ser Phe Gly Lys Thr Val Arg 
945                 950                 955                 960 


Ile Leu Asp Phe Glu Ala Tyr Glu Gln Phe Val Gln Gln Asn Tyr Ala 
                965                 970                 975     


Gly Asn Arg Pro Lys Leu Arg Asn Leu Leu Met Thr Glu Ile Pro Asn 
            980                 985                 990         


Asp Phe Ile Asp Arg Gln Leu Asn  Asp Ser Arg Tyr Ile  Ser Lys Val 
        995                 1000                 1005             


Val Lys  Gly Leu Leu Ser Asn  Ile Val Arg Glu Asp  Gly Glu Ala 
    1010                 1015                 1020             


Glu Ser  Thr Ser Lys Asn Leu  Ile Ser Cys Thr Gly  Gly Val Thr 
    1025                 1030                 1035             


Ala Arg  Leu Lys Met Asp Trp  Gly Leu Asn Asp Val  Trp Asn Asn 
    1040                 1045                 1050             


Leu Ile  Tyr Pro Arg Phe Glu  Arg Leu Asn Glu Leu  Thr Asn Ser 
    1055                 1060                 1065             


Asn Gln  Phe Gly Tyr Trp Asp  Lys Lys Asp Gly Lys  Asn Val Phe 
    1070                 1075                 1080             


Gln Thr  Gln Val Pro Met Asp  Ile Gln Lys Gly Phe  Asn Lys Lys 
    1085                 1090                 1095             


Arg Ile  Asp His Arg His His  Ala Leu Asp Ala Leu  Val Ile Ala 
    1100                 1105                 1110             


Cys Ala  Thr Arg Ser His Ile  Asn Tyr Leu Asn Asn  Glu Ser Ala 
    1115                 1120                 1125             


Lys Ser  Asn Ser Lys Glu Ser  Arg Tyr Asp Leu Arg  Arg Lys Leu 
    1130                 1135                 1140             


Arg Arg  Leu Glu Val Ile Gln  Lys Glu Tyr Thr Ile  Asn Gly Arg 
    1145                 1150                 1155             


Lys Glu  Ile Arg Asn Ile Glu  Val Ala Lys Glu Phe  Tyr Lys Pro 
    1160                 1165                 1170             


Trp Asn  Ser Phe Thr Gln Asp  Ala Gln Ser Ala Leu  Glu Asn Ile 
    1175                 1180                 1185             


Val Val  Ser Phe Lys Gln Asn  Leu Arg Val Ile Asn  Lys Thr Thr 
    1190                 1195                 1200             


Asn Tyr  Phe Leu Arg Phe Asn  Lys Glu Gly Lys Lys  Ile Phe Glu 
    1205                 1210                 1215             


Thr Gln  Thr Ser Gly Ser Asn  Trp Ala Ile Arg Lys  Pro Leu His 
    1220                 1225                 1230             


Lys Asp  Thr Ala Phe Gly Leu  Val Asn Leu Lys Arg  Thr Lys Thr 
    1235                 1240                 1245             


Val Ser  Leu Ala Asn Ala Ile  Leu Asp Tyr Asp Gln  Ile Val Asn 
    1250                 1255                 1260             


Arg Lys  Leu Trp Thr Lys Ile  Lys Glu Leu Ile Ser  Ile Gly Tyr 
    1265                 1270                 1275             


Asp Asn  Lys Arg Ile Leu Lys  Tyr Phe Thr Asp Asp  Lys Glu Asn 
    1280                 1285                 1290             


Trp Asn  Glu Ile Ile Ser Ser  Gly Ile Glu Val Phe  Tyr Tyr Thr 
    1295                 1300                 1305             


Asp Asn  Phe Glu Lys Gln Val  Ala Thr Arg Lys Pro  Leu Asp Thr 
    1310                 1315                 1320             


Ser Phe  Thr Glu Ser Lys Ile  Ile Asn Ser Ile Thr  Asp Leu Gly 
    1325                 1330                 1335             


Ile His  Lys Ile Leu Leu Ala  His Leu Arg Ala Asn  Glu Gly Asn 
    1340                 1345                 1350             


Pro Asn  Ile Ala Phe Ser Pro  Glu Gly Ile Glu Thr  Met Asn Arg 
    1355                 1360                 1365             


His Ile  Cys Glu Leu Asn Gly  Gly Lys Phe His His  Pro Ile Tyr 
    1370                 1375                 1380             


Lys Val  Arg Val His Glu Pro  Leu Gly Tyr Lys Phe  Lys Val Gly 
    1385                 1390                 1395             


Asp Lys  Gly Asn Arg Ser Thr  Lys Tyr Val Glu Ala  Ala Lys Gly 
    1400                 1405                 1410             


Thr Asn  Leu Phe Phe Gly Ile  Tyr Ile Asn Ser Gln  Gly Ile Arg 
    1415                 1420                 1425             


Ser Tyr  Glu Thr Ile Pro Leu  Asn Val Ala Ile Glu  Arg Gln Lys 
    1430                 1435                 1440             


Gln Gly  Leu Ser Pro Val Pro  Asp Asn Asn Glu Leu  Gly Glu Arg 
    1445                 1450                 1455             


Leu Leu  Phe Trp Leu Ser Pro  Asn Asp Leu Val Tyr  Leu Pro Thr 
    1460                 1465                 1470             


Ser Asp  Glu Val Glu Ser Gly  Ile Cys Cys Asn Lys  Leu Gly Ile 
    1475                 1480                 1485             


Asp Arg  Ile Tyr Lys Met Val  Ser Cys Thr Gly Tyr  Val Gly Asp 
    1490                 1495                 1500             


Phe Ile  Pro Ala His Ile Ala  Asn Pro Ile Ile Gln  Thr Leu Glu 
    1505                 1510                 1515             


Leu Gly  Ser Asn Asn Lys Ala  Gln Arg Ala Trp Thr  Gly Glu Met 
    1520                 1525                 1530             


Ile Lys  Glu Ile Cys Ile Pro  Val Lys Val Asp Arg  Met Gly Asn 
    1535                 1540                 1545             


Ile Val  Glu Ile Phe Asn Arg  Asn Lys Asn 
    1550                 1555             


<210>  1343
<211>  1564
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1343

Met Ala Lys Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      


Ala Ile Val Asp Asp Lys Asn Ser Lys Ile Leu Asp Thr Gly Val Arg 
            20                  25                  30          


Ile Phe Pro Glu Gly Val Val Ala Lys Thr Ile Gly Ser Gly Asp Lys 
        35                  40                  45              


Glu Glu Ser Lys Asn Ala Lys Arg Arg Glu Ser Arg Gln Thr Arg Arg 
    50                  55                  60                  


Gly Phe Tyr Arg Lys Arg Leu Arg Lys Ile Lys Leu Leu Arg Thr Leu 
65                  70                  75                  80  


Ile Asn Phe Lys Met Cys Pro Leu Glu His Phe Glu Leu Asp Val Trp 
                85                  90                  95      


Ser Lys Trp Asp Lys Ile Lys Lys Lys Glu Gly Arg Met Ala Pro Glu 
            100                 105                 110         


Leu Phe Leu Pro Glu Asn Ser Ala Tyr Asn Leu Trp Leu Lys Gln Asn 
        115                 120                 125             


Pro Tyr Ile Leu Arg Asp Lys Ala Leu Ser Lys Asp Leu Thr Leu Thr 
    130                 135                 140                 


Glu Phe Gly Arg Val Leu Tyr His Leu Ile Gln Arg Arg Gly Phe Leu 
145                 150                 155                 160 


Ser Asn Arg Lys Gly Lys Asp Asp Gly Lys Ile Tyr Gly Gly Lys Glu 
                165                 170                 175     


Gly Ile Val Gly Ile Asp Lys Thr Asn Glu Gln Ile Lys Asn Ser Thr 
            180                 185                 190         


Leu Gly Lys Phe Leu Phe Lys Ile Leu Pro Lys Glu Gly Glu Pro Phe 
        195                 200                 205             


Lys Ile Ile Glu Asp Glu Leu Gly Asn Glu Leu Arg Val Arg Ser Arg 
    210                 215                 220                 


Tyr Thr Leu Arg Asp Met Tyr Ile Ala Glu Phe Glu Ala Ile Trp Asn 
225                 230                 235                 240 


Arg Gln Ala Lys His Leu Gly Leu Asp Asn Ile Tyr Tyr Ser Asn Val 
                245                 250                 255     


Lys Asn Val Leu Leu Lys Gly Ser Leu Lys Asn Lys Lys Asn Glu Arg 
            260                 265                 270         


Lys Ile Asn His Leu Lys Lys Ile Lys Gly Asn Gly Asn Ile Glu Ile 
        275                 280                 285             


Ile Thr Leu Asn Asn Asn Leu Ser Lys Asn Glu Lys Pro Lys Tyr Leu 
    290                 295                 300                 


Leu Lys Ile Ala Thr Lys Leu Pro Leu Lys Glu Phe Leu Ala Gly Arg 
305                 310                 315                 320 


Ile Glu Lys Asp Glu Asp Gly Lys Ile Lys Phe Lys Ser Asn Glu Ser 
                325                 330                 335     


Val Leu Phe Trp Gln Arg Pro Leu Arg Ser Gln Lys Lys Leu Leu Ala 
            340                 345                 350         


Lys Cys Arg Phe Glu Pro Asp Leu Lys Asp Asp Asn Gly Lys Phe Ile 
        355                 360                 365             


Gln Lys Gly Lys Thr Pro Cys His Leu Ser His Pro Leu Tyr Glu Glu 
    370                 375                 380                 


Tyr Arg Ala Tyr Gln Tyr Val Asn Asn Ile Glu Tyr Gly Lys Lys Gln 
385                 390                 395                 400 


Arg Leu Glu Glu Ser Gln Arg Lys Gln Val Ile Asp Leu Ile Asn Ser 
                405                 410                 415     


Lys Asp Gly Asn Phe Lys Phe Ser Glu Ile Lys Lys Lys Leu Lys Leu 
            420                 425                 430         


Ser Tyr Glu Thr Phe Asn Tyr Ser Asp Asp Ser Ser Val Asn Gly Asn 
        435                 440                 445             


Tyr Met Ile Lys Asn Leu Asn Ser Ile Phe Pro Lys Glu Ile Leu Glu 
    450                 455                 460                 


Lys Glu Phe Val Val Asn Val Asn Lys Glu Asp Ile Ile Glu Tyr Gly 
465                 470                 475                 480 


Tyr Glu Arg Ile Trp His Leu Phe His Phe Cys Glu Asp Asn Asp Val 
                485                 490                 495     


Leu Ile Lys Lys Leu Gln Asp Asn Phe Asp Leu Ser Glu Asn Asp Ile 
            500                 505                 510         


Glu Lys Ile Ser Lys Ile Asn Leu Lys Glu Gly Tyr Ser Asn Val Ser 
        515                 520                 525             


Leu Lys Ala Ile Lys Asn Ile Leu Pro Tyr Leu Lys Glu Gly Lys Leu 
    530                 535                 540                 


Leu Ser Asp Ala Val Ile Leu Gly Gly Val Arg Asn Val Phe Gly Lys 
545                 550                 555                 560 


Lys Trp His Glu Phe Ile Asp Phe His Glu Lys Ile Glu Lys Asp Ile 
                565                 570                 575     


Leu Tyr Ile Asn His Gln Lys Ser Asn Lys Glu Gly Glu Ala Ile Glu 
            580                 585                 590         


Lys Ile Lys Lys Tyr Leu Ile Asp Tyr Lys Phe Gly Phe Thr Glu Asn 
        595                 600                 605             


Asp Asn Arg Phe Lys Lys Leu Tyr His His Ser Gln Asp Val Val Glu 
    610                 615                 620                 


Val Lys Leu Gln Lys Lys Ile Gly Pro Val Glu Asp Leu Arg Asn Pro 
625                 630                 635                 640 


Ile Val Gln Gln Gly Val Asn Glu Val Arg Arg Leu Val Asn Glu Leu 
                645                 650                 655     


Leu Asp Val His Gly Lys Phe Asp Gln Val Arg Val Glu Leu Gly Arg 
            660                 665                 670         


Asp Leu Lys Asn Asn Lys Ser Lys Arg Glu Glu Ile Gly Tyr Lys Ile 
        675                 680                 685             


Arg Glu Asn Thr Ala Lys Asn Glu Lys Ala Lys Glu Leu Leu Ile Glu 
    690                 695                 700                 


Tyr Gly Leu Arg Gln Ser Arg Asp Asn Val Gln Lys Val Leu Leu Phe 
705                 710                 715                 720 


Gln Glu Met Leu Asp Arg Gly Thr Val Ala Val Cys Pro Tyr Thr Asn 
                725                 730                 735     


Lys Thr Ile Asn Ile Ser Asp Val Leu Gly Asn Glu Asn Lys Met Gln 
            740                 745                 750         


Ile Glu His Ile Ile Pro Lys Ser Ile Ser Leu Asp Asp Ser Phe Ala 
        755                 760                 765             


Asn Lys Thr Leu Cys Asp Ser Lys Phe Asn Gly Leu Lys Gly Glu Arg 
    770                 775                 780                 


Thr Pro Tyr Glu Phe Tyr Gln Ile Asn Asn Asp Ala Asn Leu Trp Gly 
785                 790                 795                 800 


Gly Ser Lys Ser Trp Glu Glu Ile Glu Arg Arg Thr Phe Arg Ile Leu 
                805                 810                 815     


Pro Tyr Ser Lys Ala Lys Arg Phe Thr Ser Lys Thr Lys Phe Glu Thr 
            820                 825                 830         


Asn Asp Phe Ile Gln Arg Gln Leu Asn Asp Thr Arg Tyr Ile Ser Lys 
        835                 840                 845             


Lys Thr Lys Glu Ile Leu Ser Gln Ile Cys Glu Asp Val Arg Val Met 
    850                 855                 860                 


Pro Gly Ser Leu Thr Ala Glu Leu Arg Lys Leu Trp Gly Leu Asn Asn 
865                 870                 875                 880 


Ile Leu His Pro Ala Phe Val Leu Asp Leu Pro Ala Leu His Ile Asp 
                885                 890                 895     


Ala Asn Lys His Ile Pro His Tyr Val Val Leu Asp Glu Asp Asn Val 
            900                 905                 910         


Pro Ile Leu Ser Gln Arg Ile Tyr Asn Asp Lys Pro Ile Leu Lys Ala 
        915                 920                 925             


Asn Gln Thr Ser Ile Thr Gly Phe Val Asp Asn Glu Ile Phe Lys Ser 
    930                 935                 940                 


Lys Glu Lys Tyr Val Lys Phe Glu Val Lys Ala Pro Asn Leu Ser Asn 
945                 950                 955                 960 


Gly Glu Tyr Trp Ala Lys Leu Leu Leu Ser Asn Pro Lys Ser Ile Val 
                965                 970                 975     


Arg Val Phe Lys Glu Lys Pro Lys Ser Thr Glu Asn Glu Ile Val Val 
            980                 985                 990         


Arg Gly Lys Ile Glu Lys Glu Lys  Phe Lys His Glu Gly  Leu Ser Gly 
        995                 1000                 1005             


Ile Asn  Ala Glu Gly Lys Glu  Asn Gly Thr Tyr Trp  Ala Lys Leu 
    1010                 1015                 1020             


Ser Ile  Val Asp Lys Lys Phe  Lys Lys Pro Lys Lys  Asp Asp Gln 
    1025                 1030                 1035             


Pro Lys  Lys Asn Gly Lys Gln  Ile Leu Leu Phe Gly  Ala Val Lys 
    1040                 1045                 1050             


Glu Gly  Val Phe Ser Ser Tyr  Met Tyr Glu Cys Asp  Ala Asp Leu 
    1055                 1060                 1065             


Glu Asp  Gly Lys Tyr Trp Leu  Ile Leu Asp Ile Asp  Phe Glu Asn 
    1070                 1075                 1080             


Val Glu  Phe Glu Lys Ala Leu  Asn Glu Arg Pro Glu  Val Leu Glu 
    1085                 1090                 1095             


Asn Gln  Ile Leu Ile Gln Gly  Ile Thr Asn Asp Glu  Asn Val Phe 
    1100                 1105                 1110             


Val Ser  Asp Met Asp Cys Gln  His Gln Phe Leu Met  Asn Glu Gly 
    1115                 1120                 1125             


Lys Gly  Lys Tyr Tyr Val Leu  Phe Asp Ile Ile Ser  Glu Val Asn 
    1130                 1135                 1140             


Glu Phe  Tyr Pro Ile Lys Thr  Lys Ser Pro Glu Leu  Glu Asn Gly 
    1145                 1150                 1155             


Gln Ser  Leu Thr Glu Gly Thr  Ile Trp Val Asp Lys  Tyr Thr Gly 
    1160                 1165                 1170             


Glu Ile  Lys Phe Asp Pro Lys  Lys Asn Arg Glu Asp  His Arg His 
    1175                 1180                 1185             


His Ala  Ile Asp Ala Leu Val  Ile Ala Leu Thr Lys  Glu Thr Tyr 
    1190                 1195                 1200             


Phe Gln  Gln Leu Ser Arg Arg  Asn Ala Ser Ser Glu  Asn Lys Lys 
    1205                 1210                 1215             


Arg Gly  Asn Glu Tyr Glu Lys  Glu Gln Leu Asp Phe  Ala Glu Pro 
    1220                 1225                 1230             


Trp Lys  Glu Phe His Val Asp  Ala Gln Lys Ser Ile  Asn Ser Ile 
    1235                 1240                 1245             


Leu Ile  Ser His Lys Gln Asn  Lys Asn Ile Leu Thr  Lys Ile Thr 
    1250                 1255                 1260             


Lys Lys  Ile Thr Lys Asn Gly  Lys Thr Tyr Lys Ser  Glu Gly Met 
    1265                 1270                 1275             


Ser Val  Arg Gly Gln Leu His  Asp Lys Thr Tyr Tyr  Gly His Asn 
    1280                 1285                 1290             


Lys Asn  Cys Lys Ala Asp Glu  Tyr Val Tyr Arg Val  Asp Ile Asn 
    1295                 1300                 1305             


Asn Leu  Ser Phe Ser Gln Leu  Thr Asn Ile Ile Asp  Leu Asn Leu 
    1310                 1315                 1320             


Lys Ala  Val Ile Val Glu Gly  Val Ile Lys Lys Phe  Glu Glu Ser 
    1325                 1330                 1335             


Glu Lys  Leu Leu Phe Lys Lys  Met Asn Ser Lys Val  Gln Asn Leu 
    1340                 1345                 1350             


Asp Lys  Ser Lys Val Lys Lys  Ile Lys Ala Lys Val  Asp Lys Ile 
    1355                 1360                 1365             


Leu Lys  Glu Thr Ser Phe Phe  Met Pro Asn Glu Gly  Lys Arg Tyr 
    1370                 1375                 1380             


Ile Arg  Leu Asn Ile Ile Glu  Pro Ala Asn Phe Glu  Arg Met Pro 
    1385                 1390                 1395             


Val Pro  Ile Lys Lys Val Arg  Ile Arg Lys Val Leu  Arg Asn Ala 
    1400                 1405                 1410             


Glu Lys  Leu Asn Asn Glu Ile  Lys Gln Phe Val Asp  Pro Gln Asn 
    1415                 1420                 1425             


Asn His  His Val Leu Ile Tyr  Lys Asp Glu Asn Gly  Asn Leu Lys 
    1430                 1435                 1440             


Glu Glu  Val Val Thr Phe Trp  Thr Val Val Glu Arg  Lys Arg Thr 
    1445                 1450                 1455             


Gly Glu  Pro Val Tyr Lys Leu  Pro Ser Asp Gly Lys  Lys Ile Val 
    1460                 1465                 1470             


Thr Thr  Leu Gln Ile Asn Asp  Met Phe Leu Leu Gly  Leu Asn Glu 
    1475                 1480                 1485             


Asp Glu  Ile Asn Trp Glu Asn  Pro Asn Tyr Asn His  Leu Lys Glu 
    1490                 1495                 1500             


His Leu  Tyr Arg Val Gln Lys  Phe Thr Ser Gly Asp  Phe Tyr Phe 
    1505                 1510                 1515             


Arg Ile  Ala Lys Thr Ser Ser  Ile Gln Asn Gly Glu  Glu Lys Gln 
    1520                 1525                 1530             


Gln Ile  Asn Ser Phe Gly Leu  Gly Lys Asn Gly Trp  Leu Thr His 
    1535                 1540                 1545             


Asn Pro  Ile Lys Val Lys Ile  Ser Val Ser Gly Lys  Ile Leu Lys 
    1550                 1555                 1560             


Val 
    


<210>  1344
<211>  1570
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1344

Met Glu Asn Ser Asn Asn Ser Ser Lys Lys Tyr Tyr Leu Gly Leu Asp 
1               5                   10                  15      


Met Gly Thr Asp Ser Cys Gly Trp Cys Val Thr Asp Asp Lys Tyr Asn 
            20                  25                  30          


Ile Ile Arg Ser His Lys Lys Ile Tyr Gly Pro Asp Gly Lys Leu Val 
        35                  40                  45              


Lys Arg Glu Gly Cys His Leu Trp Gly Ser Arg Leu Phe Arg Pro Ala 
    50                  55                  60                  


Asn Thr Ala Lys Ser Arg Arg Leu Asn Arg Thr Ala Arg Arg Arg Leu 
65                  70                  75                  80  


Ala Arg Arg Arg Arg Arg Ile Ile Leu Leu Arg Glu Leu Phe Gln Pro 
                85                  90                  95      


Glu Ile Asp Lys Val Asp Pro Asn Phe Phe Arg Arg Leu Asp Gln Ser 
            100                 105                 110         


Asn Leu Trp Asn Glu Asp Lys Asp Lys Glu Leu Gln Met Glu His Asn 
        115                 120                 125             


Phe Leu Phe Asn Asp Lys Asp Leu Thr Asp Lys Glu Tyr Tyr Lys Asp 
    130                 135                 140                 


Phe Pro Thr Ile Tyr His Leu Arg Lys Ala Met Val Asn Gly Leu Thr 
145                 150                 155                 160 


Ile Asn Gly Tyr Tyr Tyr Asp Pro Lys Lys Gln Phe Asp Ile Arg Glu 
                165                 170                 175     


Val Tyr Leu Val Leu Ala His Met Ile Lys Tyr Arg Gly Asn Phe Leu 
            180                 185                 190         


Met Glu Gly Asp Val Lys Asp Ser Val Val Asp Pro Thr Tyr Leu Lys 
        195                 200                 205             


Gln Cys Phe Asp Asn Ile Asp Val Ile Leu Ile Ser Leu Asn Asn Gly 
    210                 215                 220                 


Glu Asp Asp Glu Asn Glu Asp Asp Lys Ile Asp Pro Ser Glu Cys Cys 
225                 230                 235                 240 


Phe Asn Val Asp Glu Lys Lys Ala Arg Lys Leu Ala Asn Asn Phe Gln 
                245                 250                 255     


Glu Thr Asn Lys Val Ser Asp Leu Glu Asp Asp Glu Arg Asp Leu Phe 
            260                 265                 270         


Gly Lys Phe Asn Ser Lys Asp Ile Arg Lys Lys Ile Leu Lys Leu Ile 
        275                 280                 285             


Asn Gly Gly Ser Gly Val Lys Leu Lys Asp Phe Leu Pro Lys Glu Ile 
    290                 295                 300                 


Leu Val Ala His Asp Gly Asp Asp Glu Tyr Gly Asp Lys Lys Ile Asp 
305                 310                 315                 320 


Leu Ser Ser Glu Thr Leu Glu Asp Asp Ile Asn Gly Tyr Ser Leu Pro 
                325                 330                 335     


Glu Ser Tyr Val Glu Ile Ile Leu Glu Ala Lys Lys Ile Tyr Asp Phe 
            340                 345                 350         


Lys Thr Leu Ala Tyr Leu Leu His Gly Lys Ser Leu Leu Cys Asp Ala 
        355                 360                 365             


Met Val Asn Val Tyr Glu Glu His Lys Glu Gln Leu Ala Ala Leu Lys 
    370                 375                 380                 


Arg Leu Tyr Lys Lys Tyr Ala Thr Lys Glu Gln Tyr Tyr Phe Met Phe 
385                 390                 395                 400 


Arg Asp Ser Arg Ile Leu Asp Gly Asp Asp Ser Asp Asp Glu Glu Asp 
                405                 410                 415     


Lys Glu Glu Ser Asn Lys Glu Glu Ser Lys Asp Lys Lys Lys Lys Ala 
            420                 425                 430         


Ser Tyr Val Ser Tyr Ile Gly Tyr Thr Lys Thr Asn Ser Lys Ala Lys 
        435                 440                 445             


Tyr Thr Gly Ala His Ile Thr Ser Glu Lys Asn Ile Ser Ala Leu Ile 
    450                 455                 460                 


Lys Gln Ile Lys Asp Asp Leu Lys Phe Asp Asp Leu Lys Ser Lys Val 
465                 470                 475                 480 


Asp Asp Pro Ser Gln Gln Asn Glu Leu Ser Asp Glu Asp Lys Leu Asp 
                485                 490                 495     


Ile Gln Cys Ile Glu Glu Ala Ile Glu Ser Asn Thr Phe Leu Pro Arg 
            500                 505                 510         


Gln Asn Ser Arg Asn Asn Gly Ala Phe Pro Tyr Gln Leu Asn Leu Asn 
        515                 520                 525             


Glu Met Arg Thr Ile Ile Glu Asn Gln Ser Lys Tyr Tyr Pro Phe Leu 
    530                 535                 540                 


Lys Asp Lys Ala Pro Ser Phe Pro Asp Gly Lys Thr Glu Glu Tyr Lys 
545                 550                 555                 560 


Ile Val Ser Leu Leu Lys Phe Lys Ile Pro Tyr Tyr Ile Gly Pro Leu 
                565                 570                 575     


Ser Gln Pro Asn Asp Tyr Val Arg Asp Asp Lys Arg Asp Asn His Trp 
            580                 585                 590         


Val Gln Phe Arg Gly Glu Asn Asn Gln Gly Ile Ile Asn Pro Trp Asn 
        595                 600                 605             


Phe Ser Gln Ile Val Asp Leu Glu Gly Thr Ala Asp Lys Phe Ile Ser 
    610                 615                 620                 


Asn Leu Thr Asn Tyr Cys Thr Tyr Ile Phe Gly Glu Asn Thr Leu Pro 
625                 630                 635                 640 


Lys Glu Ser Leu Ile Phe Gln Ala Tyr Lys Val Leu Asn Asp Leu Asn 
                645                 650                 655     


Asn Leu Lys Val Asn Gly Ser Leu Ile Ser Lys Glu Asp Lys Asp Tyr 
            660                 665                 670         


Ile Ile Gln Asn Val Tyr Leu Lys Lys Lys Lys Ile Lys Gly Lys Asp 
        675                 680                 685             


Ile Val Asn Ala Leu Lys Asn Lys Tyr Lys Leu Ser Asp Glu Asp Pro 
    690                 695                 700                 


Thr Val Thr Thr Lys Gly Ser Gly Lys Ser Ile Gly Glu Asp Glu Val 
705                 710                 715                 720 


Asp Leu Thr Met Phe Asn Ser Ser Leu Ser Ser Phe Ile Asp Met Ser 
                725                 730                 735     


Asp Pro Asn Ala Phe Gly Pro Glu Phe Tyr Lys Asp Ser Asn Lys Phe 
            740                 745                 750         


Glu Leu Ala Glu Lys Val Ile Glu Leu Ile Thr Ile Val Glu Asp Lys 
        755                 760                 765             


Asp Leu Leu Lys Glu Arg Leu Lys Lys Leu Gly Leu Ser Asp Asn Gln 
    770                 775                 780                 


Val Asn Tyr Leu Phe Lys Gln Lys Tyr Ser Gly Trp Ala Pro Leu Ser 
785                 790                 795                 800 


Arg Lys Leu Leu Asp Gly Ile Thr Ile Asp Cys Val Asn Glu Asn Thr 
                805                 810                 815     


Gly Glu Ala Tyr Pro Val Thr Ile Ile Asp Val Met Arg Asp Thr Pro 
            820                 825                 830         


Leu Asn Phe Met Glu Ile Tyr Glu Thr Lys Glu Ser Gln Phe Ser Gly 
        835                 840                 845             


Phe Lys Glu Lys Val Ala Glu Leu Asn Asp Glu Lys Lys Leu Ser Arg 
    850                 855                 860                 


Asp Asp Leu Ile Glu Glu Ser Tyr Ala Ser Pro Ala Met Lys Arg Ser 
865                 870                 875                 880 


Val Arg Gln Thr Phe Lys Val Ile Asp Glu Leu Lys Asn Thr Leu Asn 
                885                 890                 895     


Ile Asp His Phe Asp Arg Ile Phe Val Glu Ala Thr Arg Ala Pro Gly 
            900                 905                 910         


Glu Ala Gly Lys Met Val Lys Ser Arg Lys Lys Gln Leu Gln Glu Ala 
        915                 920                 925             


Tyr Asp Ala Ala Lys Asp Leu Val Asp Gln Glu Ser Arg Asp Asp Leu 
    930                 935                 940                 


Gln Lys Leu Arg Asp Asp Leu Glu Lys Gln Thr Asp Asp Gln Leu Arg 
945                 950                 955                 960 


Arg Lys Lys Val Tyr Leu Tyr Phe Met Gln Leu Gly Arg Ser Val Tyr 
                965                 970                 975     


Ser Gly Glu Lys Ile Asp Leu Asn Glu Leu Asp Lys Asn Tyr Asp Ile 
            980                 985                 990         


Asp His Ile Ile Pro Gln Ala Lys  Leu Lys Asp Asp Ser  Phe Asn Asn 
        995                 1000                 1005             


Met Val  Leu Val Glu Arg Glu  Leu Asn Asn Lys Lys  Gln Asp Glu 
    1010                 1015                 1020             


Tyr Pro  Ile Pro Ser Thr Ile  Ile Ser Glu His Gly  Arg Asn Trp 
    1025                 1030                 1035             


Ile Gln  Lys Leu Ala Lys Ile  Gln Asp Lys Asn Lys  Arg Ser Tyr 
    1040                 1045                 1050             


Phe Met  Pro Lys Asp Lys Ala  Asn Arg Leu Leu Arg  Pro Glu Ser 
    1055                 1060                 1065             


Lys Gln  Leu Thr Ser Asp Glu  Leu Asn Gly Phe Ile  Asn Arg Gln 
    1070                 1075                 1080             


Leu Thr  Leu Thr Asn Gln Ser  Val Lys Ala Val Cys  Asp Ile Leu 
    1085                 1090                 1095             


Lys Met  Thr Glu Lys Gly Ser  Glu Ile Val Tyr Ser  Lys Ala Asn 
    1100                 1105                 1110             


Phe Val  Ser Asp Phe Arg Lys  Leu Cys Gly Glu Leu  Arg Pro Ser 
    1115                 1120                 1125             


Tyr Asp  Ala Phe Val Lys Val  Arg Asp Ile Asn Asp  Phe His His 
    1130                 1135                 1140             


Ala Asn  Asp Ala Tyr Leu Asn  Ile Val Val Gly Asn  Val Tyr Tyr 
    1145                 1150                 1155             


Glu Lys  Phe Lys Asn Phe Tyr  Ile Asn Asn Arg Ile  Asp Tyr Asp 
    1160                 1165                 1170             


Asn Asn  Gly Lys Lys Asp Leu  Ser Val Leu Ala Ser  Lys Leu Phe 
    1175                 1180                 1185             


Thr Lys  Thr Gln Ala Asp Asp  Lys Thr Gly Asn Ile  Tyr Trp Glu 
    1190                 1195                 1200             


Ala Arg  Phe His Asn Glu Lys  Glu Thr Asp Ser Asn  Gly Lys Glu 
    1205                 1210                 1215             


Arg Thr  Ile Lys Val Arg Asp  Glu Gly Thr Glu Ser  Thr Phe Asp 
    1220                 1225                 1230             


Lys Ile  Glu Tyr Tyr Met Glu  Leu Asn Asp Pro Leu  Val Thr Gln 
    1235                 1240                 1245             


Met Gln  Tyr Thr Leu Ser Gly  Lys Gln Gly Phe Phe  Lys Lys Ile 
    1250                 1255                 1260             


Thr Leu  Lys Thr Lys Ala Lys  Asp Asn Glu Asp Asn  Ser Asp Lys 
    1265                 1270                 1275             


Leu Phe  Pro Leu Lys Gln Tyr  Leu Arg Ser Asp Ser  Asn Lys Val 
    1280                 1285                 1290             


Met Asp  Val Asn Lys Tyr Gly  Gly Tyr Ser Asp Met  Ile Thr Pro 
    1295                 1300                 1305             


Tyr Phe  Met Leu Val Gln Ser  Glu Gly Lys Lys Gly  Lys His Ile 
    1310                 1315                 1320             


Tyr Ser  Leu Glu Ser Ile Pro  Thr Ile Tyr Leu Lys  Ser Phe Lys 
    1325                 1330                 1335             


Lys Lys  Glu Glu Asp Ile Ile  Asn Tyr Leu Ser Cys  Lys Asn Lys 
    1340                 1345                 1350             


Leu Lys  Asn Pro Val Val Ile  Ser Asp Lys Leu Leu  Met Arg Thr 
    1355                 1360                 1365             


Ile Val  Lys Ile Pro Tyr Phe  Lys Asn Asp Ser Asn  Leu Ser Leu 
    1370                 1375                 1380             


Val Lys  Leu Gly Thr Ser Gly  Arg Thr Gly Asn Asn  Ile Ile Tyr 
    1385                 1390                 1395             


Ile Asn  Leu Ser Glu Leu Ile  Leu Pro Ile Glu Trp  Lys Asn Tyr 
    1400                 1405                 1410             


Tyr Arg  Lys Ile Ser Asn Leu  Leu Gly Thr Asn Val  Ser Ser Thr 
    1415                 1420                 1425             


Lys Pro  Lys Asp Leu Ser Lys  Phe Thr Glu Gln Met  Lys Asn Asp 
    1430                 1435                 1440             


Pro Thr  Ser Thr Leu Val Asp  Gly Ser Asn Lys Val  Glu His Asp 
    1445                 1450                 1455             


Lys Asn  Asn Arg Phe Phe Ser  Tyr Leu Ile Asn Asn  Val Ile Asn 
    1460                 1465                 1470             


Lys Pro  Ile Phe Glu Gly Leu  Pro Lys Thr Ser Cys  Ser Phe Ile 
    1475                 1480                 1485             


Lys Leu  Glu Ser Lys Tyr Asp  Glu Phe Cys Ser Leu  Asn Thr Leu 
    1490                 1495                 1500             


Glu Gln  Ala Lys Ile Ile Ala  Thr Ile Ile Lys Leu  Leu Ser Cys 
    1505                 1510                 1515             


Lys Ser  Val Gln Gly Cys Lys  Leu Ser Asn Leu Ser  Gly Leu Pro 
    1520                 1525                 1530             


Ser Ser  Leu Gly Ala Leu Ile  Ser Asn Lys Ala Leu  Thr Glu Gly 
    1535                 1540                 1545             


Thr Ile  Ile Ser Lys Glu Ser  Ser Cys Gly Leu Lys  Glu Asn Ile 
    1550                 1555                 1560             


Ile Phe  Val Val Pro Asn Asp  
    1565                 1570 


<210>  1345
<211>  1688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  1345

Met Glu Phe Lys Lys Val Leu Gly Leu Asp Ile Gly Thr Asn Ser Ile 
1               5                   10                  15      


Gly Cys Ala Leu Leu Ser Leu Pro Lys Ser Ile Gln Asp Tyr Gly Lys 
            20                  25                  30          


Gly Gly Arg Leu Glu Trp Leu Thr Ser Arg Val Ile Pro Leu Asp Ala 
        35                  40                  45              


Asp Tyr Met Lys Ala Phe Ile Asp Gly Lys Asn Gly Leu Pro Gln Val 
    50                  55                  60                  


Ile Thr Pro Ala Gly Lys Arg Arg Gln Lys Arg Gly Ser Arg Arg Leu 
65                  70                  75                  80  


Lys His Arg Tyr Lys Leu Arg Arg Ser Arg Leu Ile Arg Val Phe Lys 
                85                  90                  95      


Thr Leu Asn Trp Leu Pro Glu Asp Phe Pro Leu Asp Asn Pro Lys Arg 
            100                 105                 110         


Ile Lys Glu Thr Ile Ser Thr Glu Gly Lys Phe Ser Phe Arg Ile Ser 
        115                 120                 125             


Asp Tyr Val Pro Ile Ser Asp Glu Ser Tyr Arg Glu Phe Tyr Arg Glu 
    130                 135                 140                 


Phe Gly Tyr Pro Glu Asn Glu Ile Glu Gln Val Ile Glu Glu Ile Asn 
145                 150                 155                 160 


Phe Arg Arg Lys Thr Lys Gly Lys Asn Lys Asn Pro Met Ile Lys Leu 
                165                 170                 175     


Leu Pro Glu Asp Trp Val Val Tyr Tyr Leu Arg Lys Lys Ala Leu Ile 
            180                 185                 190         


Lys Pro Thr Thr Lys Glu Glu Leu Ile Arg Ile Ile Tyr Leu Phe Asn 
        195                 200                 205             


Gln Arg Arg Gly Phe Lys Ser Ser Arg Lys Asp Leu Thr Glu Thr Ala 
    210                 215                 220                 


Ile Leu Asp Tyr Asp Glu Phe Ala Lys Arg Leu Ala Glu Lys Glu Lys 
225                 230                 235                 240 


Tyr Ser Ala Glu Asn Tyr Glu Thr Lys Phe Val Ser Ile Thr Lys Val 
                245                 250                 255     


Lys Glu Val Val Glu Leu Lys Thr Asp Gly Arg Lys Gly Lys Lys Arg 
            260                 265                 270         


Phe Lys Val Ile Leu Glu Asp Ser Arg Ile Glu Pro Tyr Glu Ile Glu 
        275                 280                 285             


Arg Lys Glu Lys Pro Asp Trp Glu Gly Lys Glu Tyr Thr Phe Leu Val 
    290                 295                 300                 


Thr Gln Lys Leu Glu Lys Gly Lys Phe Lys Gln Asn Lys Pro Asp Leu 
305                 310                 315                 320 


Pro Lys Glu Glu Asp Trp Ala Leu Cys Thr Thr Ala Leu Asp Asn Arg 
                325                 330                 335     


Met Gly Ser Lys His Pro Gly Glu Phe Phe Phe Asp Glu Leu Leu Lys 
            340                 345                 350         


Ala Phe Lys Glu Lys Arg Gly Tyr Lys Ile Arg Gln Tyr Pro Val Asn 
        355                 360                 365             


Arg Trp Arg Tyr Lys Lys Glu Leu Glu Phe Ile Trp Thr Lys Gln Cys 
    370                 375                 380                 


Gln Leu Asn Pro Glu Leu Asn Asn Leu Asn Ile Asn Lys Glu Ile Leu 
385                 390                 395                 400 


Arg Lys Leu Ala Thr Val Leu Tyr Pro Ser Gln Ser Lys Phe Phe Gly 
                405                 410                 415     


Pro Lys Ile Lys Glu Phe Glu Asn Ser Asp Val Leu His Ile Ile Ser 
            420                 425                 430         


Glu Asp Ile Ile Tyr Tyr Gln Arg Asp Leu Lys Ser Gln Lys Ser Leu 
        435                 440                 445             


Ile Ser Glu Cys Arg Tyr Glu Lys Arg Lys Gly Ile Asp Gly Glu Ile 
    450                 455                 460                 


Tyr Gly Leu Lys Cys Ile Pro Lys Ser Ser Pro Leu Tyr Gln Glu Phe 
465                 470                 475                 480 


Arg Ile Trp Gln Asp Ile His Asn Ile Lys Val Ile Arg Lys Glu Ser 
                485                 490                 495     


Glu Val Asn Gly Lys Lys Lys Ile Asn Ile Asp Glu Thr Gln Leu Tyr 
            500                 505                 510         


Ile Asn Glu Asn Ile Lys Glu Lys Leu Phe Glu Leu Phe Asn Ser Lys 
        515                 520                 525             


Asp Ser Leu Ser Glu Lys Asp Ile Leu Glu Leu Ile Ser Leu Asn Ile 
    530                 535                 540                 


Ile Asn Ser Gly Ile Lys Ile Ser Lys Lys Glu Glu Glu Thr Thr His 
545                 550                 555                 560 


Arg Ile Asn Leu Phe Ala Asn Arg Lys Glu Leu Lys Gly Asn Glu Thr 
                565                 570                 575     


Lys Ser Arg Tyr Arg Lys Val Phe Lys Lys Leu Gly Phe Asp Gly Glu 
            580                 585                 590         


Tyr Ile Leu Asn His Pro Ser Lys Leu Asn Arg Leu Trp His Ser Asp 
        595                 600                 605             


Tyr Ser Asn Asp Tyr Ala Asp Lys Glu Lys Thr Glu Lys Ser Ile Leu 
    610                 615                 620                 


Ser Ser Leu Gly Trp Lys Asn Arg Asn Gly Lys Trp Glu Lys Ser Lys 
625                 630                 635                 640 


Asn Tyr Asp Val Phe Asn Leu Pro Leu Glu Val Ala Lys Ala Ile Ala 
                645                 650                 655     


Asn Leu Pro Pro Leu Lys Lys Glu Tyr Gly Ser Tyr Ser Ala Leu Ala 
            660                 665                 670         


Ile Arg Lys Met Leu Val Val Met Arg Asp Gly Lys Tyr Trp Gln His 
        675                 680                 685             


Pro Asp Gln Ile Ala Lys Asp Gln Glu Asn Thr Ser Leu Met Leu Phe 
    690                 695                 700                 


Asp Lys Asn Leu Ile Gln Leu Thr Asn Asn Gln Arg Lys Val Leu Asn 
705                 710                 715                 720 


Lys Tyr Leu Leu Thr Leu Ala Glu Val Gln Lys Arg Ser Thr Leu Ile 
                725                 730                 735     


Lys Gln Lys Leu Asn Glu Ile Glu His Asn Pro Tyr Lys Leu Glu Leu 
            740                 745                 750         


Val Ser Asp Gln Asp Leu Glu Lys Gln Val Leu Lys Ser Phe Leu Glu 
        755                 760                 765             


Lys Lys Asn Glu Ser Asp Tyr Leu Lys Gly Leu Lys Thr Tyr Gln Ala 
    770                 775                 780                 


Gly Tyr Leu Ile Tyr Gly Lys His Ser Glu Lys Asp Val Pro Ile Val 
785                 790                 795                 800 


Asn Ser Pro Asp Glu Leu Gly Glu Tyr Ile Arg Lys Lys Leu Pro Asn 
                805                 810                 815     


Asn Ser Leu Arg Asn Pro Ile Val Glu Gln Val Ile Arg Glu Thr Ile 
            820                 825                 830         


Phe Ile Val Arg Asp Val Trp Lys Ser Phe Gly Ile Ile Asp Glu Ile 
        835                 840                 845             


His Ile Glu Leu Gly Arg Glu Leu Lys Asn Asn Ser Glu Glu Arg Lys 
    850                 855                 860                 


Lys Thr Ser Glu Ser Gln Glu Lys Asn Phe Gln Glu Lys Glu Arg Ala 
865                 870                 875                 880 


Arg Lys Leu Leu Lys Glu Leu Leu Asn Ser Ser Asn Phe Glu His Tyr 
                885                 890                 895     


Asp Glu Asn Gly Asn Lys Ile Phe Ser Ser Phe Thr Val Asn Pro Asn 
            900                 905                 910         


Pro Asp Ser Pro Leu Asp Ile Glu Lys Phe Arg Ile Trp Lys Asn Gln 
        915                 920                 925             


Ser Gly Leu Thr Asp Glu Glu Leu Asn Lys Lys Leu Lys Asp Glu Lys 
    930                 935                 940                 


Ile Pro Thr Glu Ile Glu Val Lys Lys Tyr Ile Leu Trp Leu Thr Gln 
945                 950                 955                 960 


Lys Cys Arg Ser Pro Tyr Thr Gly Lys Ile Ile Pro Leu Ser Lys Leu 
                965                 970                 975     


Phe Asp Ser Asn Val Tyr Glu Ile Glu His Ile Ile Pro Arg Ser Lys 
            980                 985                 990         


Met Lys Asn Asp Ser Thr Asn Asn  Leu Val Ile Cys Glu  Leu Gly Val 
        995                 1000                 1005             


Asn Lys  Ala Lys Gly Asp Arg  Leu Ala Ala Asn Phe  Ile Ser Glu 
    1010                 1015                 1020             


Ser Asn  Gly Lys Cys Lys Phe  Gly Glu Val Glu Tyr  Thr Leu Leu 
    1025                 1030                 1035             


Lys Tyr  Gly Asp Tyr Leu Gln  Tyr Cys Lys Asp Thr  Phe Lys Tyr 
    1040                 1045                 1050             


Gln Lys  Ala Lys Tyr Lys Asn  Leu Leu Ala Thr Glu  Pro Pro Glu 
    1055                 1060                 1065             


Asp Phe  Ile Glu Arg Gln Ile  Asn Asp Thr Arg Tyr  Ile Gly Arg 
    1070                 1075                 1080             


Lys Leu  Ala Glu Leu Leu Thr  Pro Val Val Lys Asp  Ser Lys Asn 
    1085                 1090                 1095             


Ile Ile  Phe Thr Ile Gly Ser  Ile Thr Ser Glu Leu  Lys Ile Thr 
    1100                 1105                 1110             


Trp Gly  Leu Asn Gly Val Trp  Lys Asp Ile Leu Arg  Pro Arg Phe 
    1115                 1120                 1125             


Lys Arg  Leu Glu Ser Ile Ile  Asn Lys Lys Leu Ile  Phe Gln Asp 
    1130                 1135                 1140             


Glu Asp  Asp Pro Asn Lys Tyr  His Phe Asp Leu Ser  Ile Asn Pro 
    1145                 1150                 1155             


Gln Leu  Asp Lys Glu Gly Leu  Lys Arg Leu Asp His  Arg His His 
    1160                 1165                 1170             


Ala Leu  Asp Ala Thr Ile Ile  Ala Ala Thr Thr Arg  Glu His Val 
    1175                 1180                 1185             


Arg Tyr  Leu Asn Ser Leu Asn  Ala Ala Asp Asn Asp  Glu Glu Lys 
    1190                 1195                 1200             


Arg Glu  Tyr Phe Leu Ser Leu  Cys Asn His Lys Ile  Arg Asp Phe 
    1205                 1210                 1215             


Lys Leu  Pro Trp Glu Asn Phe  Thr Ser Glu Val Lys  Ser Lys Leu 
    1220                 1225                 1230             


Leu Ser  Cys Val Val Ser Tyr  Lys Glu Ser Lys Pro  Ile Leu Ser 
    1235                 1240                 1245             


Asp Pro  Phe Asn Lys Tyr Leu  Lys Trp Glu Tyr Lys  Asn Gly Lys 
    1250                 1255                 1260             


Trp Gln  Lys Val Phe Ala Ile  Gln Ile Lys Asn Asp  Arg Trp Lys 
    1265                 1270                 1275             


Ala Val  Arg Arg Ser Met Phe  Lys Glu Pro Ile Gly  Thr Val Trp 
    1280                 1285                 1290             


Ile Lys  Lys Ile Lys Glu Val  Ser Leu Lys Glu Ala  Ile Lys Ile 
    1295                 1300                 1305             


Gln Ala  Ile Trp Glu Glu Val  Lys Asn Asp Pro Val  Arg Lys Lys 
    1310                 1315                 1320             


Lys Glu  Lys Tyr Ile Tyr Asp  Asp Tyr Ala Gln Lys  Val Ile Ala 
    1325                 1330                 1335             


Lys Ile  Val Gln Glu Leu Gly  Leu Ser Ser Ser Met  Arg Lys Gln 
    1340                 1345                 1350             


Asp Asp  Glu Lys Leu Asn Lys  Phe Ile Asn Glu Ala  Lys Val Ser 
    1355                 1360                 1365             


Ala Gly  Val Asn Lys Asn Leu  Asn Thr Thr Asn Lys  Thr Ile Tyr 
    1370                 1375                 1380             


Asn Leu  Glu Gly Arg Phe Tyr  Glu Lys Ile Lys Val  Ala Glu Tyr 
    1385                 1390                 1395             


Val Leu  Tyr Lys Ala Lys Arg  Met Pro Leu Asn Lys  Lys Glu Tyr 
    1400                 1405                 1410             


Ile Glu  Lys Leu Ser Leu Gln  Lys Met Phe Asn Asp  Leu Pro Asn 
    1415                 1420                 1425             


Phe Ile  Leu Glu Lys Ser Ile  Leu Asp Asn Tyr Pro  Glu Ile Leu 
    1430                 1435                 1440             


Lys Glu  Leu Glu Ser Asp Asn  Lys Tyr Ile Ile Glu  Pro His Lys 
    1445                 1450                 1455             


Lys Asn  Asn Pro Val Asn Arg  Leu Leu Leu Glu His  Ile Leu Glu 
    1460                 1465                 1470             


Tyr His  Asn Asn Pro Lys Glu  Ala Phe Ser Thr Glu  Gly Leu Glu 
    1475                 1480                 1485             


Lys Leu  Asn Lys Lys Ala Ile  Asn Lys Ile Gly Lys  Pro Ile Lys 
    1490                 1495                 1500             


Tyr Ile  Thr Arg Leu Asp Gly  Asp Ile Asn Glu Glu  Glu Ile Phe 
    1505                 1510                 1515             


Arg Gly  Ala Val Phe Glu Thr  Asp Lys Gly Ser Asn  Val Tyr Phe 
    1520                 1525                 1530             


Val Met  Tyr Glu Asn Asn Gln  Thr Lys Asp Arg Glu  Phe Leu Lys 
    1535                 1540                 1545             


Pro Asn  Pro Ser Ile Ser Val  Leu Lys Ala Ile Glu  His Lys Asn 
    1550                 1555                 1560             


Lys Ile  Asp Phe Phe Ala Pro  Asn Arg Leu Gly Phe  Ser Arg Ile 
    1565                 1570                 1575             


Ile Leu  Ser Pro Gly Asp Leu  Val Tyr Val Pro Thr  Asn Asp Gln 
    1580                 1585                 1590             


Tyr Val  Leu Ile Lys Asp Asn  Ser Ser Asn Glu Thr  Ile Ile Asn 
    1595                 1600                 1605             


Trp Asp  Asp Asn Glu Phe Ile  Ser Asn Arg Ile Tyr  Gln Val Lys 
    1610                 1615                 1620             


Lys Phe  Thr Gly Asn Ser Cys  Tyr Phe Leu Lys Asn  Asp Ile Ala 
    1625                 1630                 1635             


Ser Leu  Ile Leu Ser Tyr Ser  Ala Ser Asn Gly Val  Gly Glu Phe 
    1640                 1645                 1650             


Gly Ser  Gln Asn Ile Ser Glu  Tyr Ser Val Asp Asp  Pro Pro Ile 
    1655                 1660                 1665             


Arg Ile  Lys Asp Val Cys Ile  Lys Ile Arg Val Asp  Arg Leu Gly 
    1670                 1675                 1680             


Asn Val  Arg Pro Leu 
    1685             


<210>  1346
<211>  1307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1346

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn 
    1295                 1300                 1305         


<210>  1347
<211>  1300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1347

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  1348
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1348

Met Lys Pro Asp Ile Lys Asp Arg Glu Tyr Met Tyr Arg Leu Ile Ile 
1               5                   10                  15      


Gly Gln Leu Phe Tyr Asp Gly His Gln Gln Ile Ala Val Asn Leu Ala 
            20                  25                  30          


Asn Thr Leu Gly Cys Ser Ala Pro Pro Pro Pro Pro Ser Asp Lys Leu 
        35                  40                  45              


Phe Arg Leu Val Thr Ile Ala Lys Gln Phe Val Glu Asp Pro Asp Tyr 
    50                  55                  60                  


Lys Ala Glu Glu Asn Thr Met Pro Met Gln Phe Glu Thr Ser Ser Ala 
65                  70                  75                  80  


Gly Leu Asp Leu Gln Tyr Asp Ala Asp Val Ala Ala Thr Ser Pro Glu 
                85                  90                  95      


Pro Ser Gln Tyr Glu Thr Ile Tyr Leu Thr Val His Lys Ala Pro Cys 
            100                 105                 110         


Arg Ala Ala Ala Phe Asn Ser Asp Gly Ser Leu Ile Ala Thr Gly Ser 
        115                 120                 125             


Ala Asp Cys Ser Ile Lys Ile Met Asp Val Glu Arg Ile Leu Ala Arg 
    130                 135                 140                 


Glu Lys Glu His Arg Glu Met Asn Glu Asn Gly Pro Asp Ala His His 
145                 150                 155                 160 


Pro Val Ile Arg Thr Leu Tyr Asp His Val Asp Val Arg Arg Asp Phe 
                165                 170                 175     


Gly Arg Phe Ser Asp Lys Ile Leu Asp Phe Gln Asp Val Asn Thr Val 
            180                 185                 190         


Ile Phe His Pro Arg Asp Ala Ile Leu Ile Ser Gly Ser Asn Asp Lys 
        195                 200                 205             


Thr Val Lys Leu Phe Asp Phe Ser Lys Thr Ala Val Lys Arg Ala Met 
    210                 215                 220                 


Lys Thr Leu Ser Glu Val Tyr Pro Val Arg Ala Leu Ser Cys His Pro 
225                 230                 235                 240 


Gly Gly Glu Phe Leu Leu Val Ala Thr Asp His Pro Thr Val Arg Leu 
                245                 250                 255     


Tyr Asn Ile Glu Thr Ala Gln Ala Tyr Ala Ser Ala Asn Pro Asp Asp 
            260                 265                 270         


Gln His Arg Glu Ser Val Thr Asp Val His Tyr Ser Glu Asn Ala Arg 
        275                 280                 285             


Leu Tyr Val Thr Ala Ser Lys Asp Gly Asp Val Lys Val Trp Asp Gly 
    290                 295                 300                 


Val Ser Ser Arg Cys Val Glu Thr Phe Lys Arg Ala His Asp Gly Ser 
305                 310                 315                 320 


Ser Ile Cys Ser Ala Lys Phe Thr Lys Asn Gly Lys Tyr Ile Leu Thr 
                325                 330                 335     


Ser Gly Met Asp Ser Ile Val Lys Leu Trp Glu Leu Ser Thr Asn Arg 
            340                 345                 350         


Cys Leu Ile Val Tyr Thr Gly Ala Gly Ala Thr Gly Ala Gln Asp Tyr 
        355                 360                 365             


Pro Thr Ser Ala Ser Phe Asn Gln Asn Glu Asp Phe Val Leu Phe Pro 
    370                 375                 380                 


Asp Glu Lys Ser Gly Ser Met Cys Ser Trp Glu Ala Arg Asn Ala Asp 
385                 390                 395                 400 


Arg Lys Arg Leu Leu Ala Leu Gly His Thr Ser Ala Cys Arg Thr Phe 
                405                 410                 415     


Val His Ser Pro Ser Met Pro Ala Phe Met Thr Gly Ser Asp Asp His 
            420                 425                 430         


Arg Ala Arg Phe Trp Tyr Arg Lys Pro Thr Thr Asn Asp Asn 
        435                 440                 445     


<210>  1349
<211>  430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1349

Met Lys Pro Asp Ile Lys Asp Arg Glu Tyr Met Tyr Arg Leu Ile Ile 
1               5                   10                  15      


Gly Gln Leu Phe Tyr Asp Gly His Gln Gln Ile Ala Val Thr Leu Ala 
            20                  25                  30          


Asn Thr Leu Gly Cys Ser Ala Pro Ala Pro Pro Pro Ser Asp Lys Leu 
        35                  40                  45              


Tyr Arg Leu Val Thr Ile Ala Lys Gln Phe Val Glu Asp Pro Asp Tyr 
    50                  55                  60                  


Lys Ala Glu Glu Lys Ser Leu Pro Met Gln Phe Glu Thr Val Ser Thr 
65                  70                  75                  80  


Gly Leu Asp Leu Glu Tyr Asp Ala Asp Val Gln Pro Val Ser Pro Glu 
                85                  90                  95      


Pro Ser Gln Tyr Glu Thr Ile Tyr Leu Thr Val His Lys Ala Pro Cys 
            100                 105                 110         


Arg Ala Ala Ala Phe Asn Ser Asp Gly Ser Leu Val Ala Thr Gly Ser 
        115                 120                 125             


Ala Asp Cys Ser Ile Lys Ile Met Asp Val Glu Arg Ile Leu Ala Arg 
    130                 135                 140                 


Glu Lys Glu His Arg Glu Met Asn Glu Asn Gly Pro Asp Ala His His 
145                 150                 155                 160 


Pro Val Ile Arg Thr Leu Tyr Asp His Val Asp Asp Val Asn Thr Val 
                165                 170                 175     


Leu Phe His Pro Arg Asp Ala Ile Leu Ile Ser Gly Ser Asn Asp Lys 
            180                 185                 190         


Thr Val Lys Leu Phe Asp Phe Ser Lys Thr Ala Val Lys Arg Ala Met 
        195                 200                 205             


Lys Thr Leu Ser Glu Val Tyr Pro Val Arg Ala Leu Ser Cys His Pro 
    210                 215                 220                 


Gly Gly Glu Phe Leu Leu Val Ala Thr Asp His Pro Thr Val Arg Leu 
225                 230                 235                 240 


Tyr Asn Ile Glu Thr Ala Gln Ala Tyr Ala Ser Ala Asn Pro Asp Asp 
                245                 250                 255     


Gln His Arg Glu Ser Val Thr Asp Val His Tyr Ser Glu Asn Ala Arg 
            260                 265                 270         


Leu Tyr Val Thr Ala Ser Lys Asp Gly Asp Val Lys Ile Trp Asp Gly 
        275                 280                 285             


Val Ser Asn Arg Cys Val Glu Thr Phe Lys Arg Ala His Asp Gly Ser 
    290                 295                 300                 


Ala Ile Cys Ser Ala Lys Phe Thr Lys Asn Gly Lys Tyr Leu Leu Thr 
305                 310                 315                 320 


Ser Gly Met Asp Ser Ile Val Lys Leu Trp Glu Leu Ser Thr Asn Arg 
                325                 330                 335     


Cys Leu Ile Val Tyr Thr Gly Ala Gly Ala Thr Gly Ala Gln Asp Tyr 
            340                 345                 350         


Ala Thr Ser Ala Ser Phe Asn His Asn Glu Asp Tyr Val Leu Phe Pro 
        355                 360                 365             


Asp Glu Lys Ser Gly Ser Met Cys Ser Trp Asp Ala Arg Asn Ser Asp 
    370                 375                 380                 


Arg Lys Arg Leu Leu Ala Leu Gly His Thr Ser Ala Cys Arg Thr Phe 
385                 390                 395                 400 


Val His Ser Pro Ser Met Pro Ala Phe Met Thr Gly Ser Asp Asp His 
                405                 410                 415     


Arg Ala Arg Phe Trp Tyr Arg Lys Pro Ala Thr Thr Asp Asn 
            420                 425                 430 


<210>  1350
<211>  1228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1350

Ala Ala Ser Lys Leu Glu Lys Phe Thr Asn Cys Tyr Ser Leu Ser Lys 
1               5                   10                  15      


Thr Leu Arg Phe Lys Ala Ile Pro Val Gly Lys Thr Gln Glu Asn Ile 
            20                  25                  30          


Asp Asn Lys Arg Leu Leu Val Glu Asp Glu Lys Arg Ala Glu Asp Tyr 
        35                  40                  45              


Lys Gly Val Lys Lys Leu Leu Asp Arg Tyr Tyr Leu Ser Phe Ile Asn 
    50                  55                  60                  


Asp Val Leu His Ser Ile Lys Leu Lys Asn Leu Asn Asn Tyr Ile Ser 
65                  70                  75                  80  


Leu Phe Arg Lys Lys Thr Arg Thr Glu Lys Glu Asn Lys Glu Leu Glu 
                85                  90                  95      


Asn Leu Glu Ile Asn Leu Arg Lys Glu Ile Ala Lys Ala Phe Lys Gly 
            100                 105                 110         


Ala Ala Gly Tyr Lys Ser Leu Phe Lys Lys Asp Ile Ile Glu Thr Ile 
        115                 120                 125             


Leu Pro Glu Ala Ala Asp Asp Lys Asp Glu Ile Ala Leu Val Asn Ser 
    130                 135                 140                 


Phe Asn Gly Phe Thr Thr Ala Phe Thr Gly Phe Phe Asp Asn Arg Glu 
145                 150                 155                 160 


Asn Met Phe Ser Glu Glu Ala Lys Ser Thr Ser Ile Ala Phe Arg Cys 
                165                 170                 175     


Ile Asn Glu Asn Leu Thr Arg Tyr Ile Ser Asn Met Asp Ile Phe Glu 
            180                 185                 190         


Lys Val Asp Ala Ile Phe Asp Lys His Glu Val Gln Glu Ile Lys Glu 
        195                 200                 205             


Lys Ile Leu Asn Ser Asp Tyr Asp Val Glu Asp Phe Phe Glu Gly Glu 
    210                 215                 220                 


Phe Phe Asn Phe Val Leu Thr Gln Glu Gly Ile Asp Val Tyr Asn Ala 
225                 230                 235                 240 


Ile Ile Gly Gly Phe Val Thr Glu Ser Gly Glu Lys Ile Lys Gly Leu 
                245                 250                 255     


Asn Glu Tyr Ile Asn Leu Tyr Asn Ala Lys Thr Lys Gln Ala Leu Pro 
            260                 265                 270         


Lys Phe Lys Pro Leu Tyr Lys Gln Val Leu Ser Asp Arg Glu Ser Leu 
        275                 280                 285             


Ser Phe Tyr Gly Glu Gly Tyr Thr Ser Asp Glu Glu Val Leu Glu Val 
    290                 295                 300                 


Phe Arg Asn Thr Leu Asn Lys Asn Ser Glu Ile Phe Ser Ser Ile Lys 
305                 310                 315                 320 


Lys Leu Glu Lys Leu Phe Lys Asn Phe Asp Glu Tyr Ser Ser Ala Gly 
                325                 330                 335     


Ile Phe Val Lys Asn Gly Pro Ala Ile Ser Thr Ile Ser Lys Asp Ile 
            340                 345                 350         


Phe Gly Glu Trp Asn Leu Ile Arg Asp Lys Trp Asn Ala Glu Tyr Asp 
        355                 360                 365             


Asp Ile His Leu Lys Lys Lys Ala Val Val Thr Glu Lys Tyr Glu Asp 
    370                 375                 380                 


Asp Arg Arg Lys Ser Phe Lys Lys Ile Gly Ser Phe Ser Leu Glu Gln 
385                 390                 395                 400 


Leu Gln Glu Tyr Ala Asp Ala Asp Leu Ser Val Val Glu Lys Leu Lys 
                405                 410                 415     


Glu Ile Ile Ile Gln Lys Val Asp Glu Ile Tyr Lys Val Tyr Gly Ser 
            420                 425                 430         


Ser Glu Lys Leu Phe Asp Ala Asp Phe Val Leu Glu Lys Ser Leu Lys 
        435                 440                 445             


Lys Asn Asp Ala Val Val Ala Ile Met Lys Asp Leu Leu Asp Ser Val 
    450                 455                 460                 


Lys Ser Phe Glu Asn Tyr Ile Lys Ala Phe Phe Gly Glu Gly Lys Glu 
465                 470                 475                 480 


Thr Asn Arg Asp Glu Ser Phe Tyr Gly Asp Phe Val Leu Ala Tyr Asp 
                485                 490                 495     


Ile Leu Leu Lys Val Asp His Ile Tyr Asp Ala Ile Arg Asn Tyr Val 
            500                 505                 510         


Thr Gln Lys Pro Tyr Ser Lys Asp Lys Phe Lys Leu Tyr Phe Gln Asn 
        515                 520                 525             


Pro Gln Phe Met Gly Gly Trp Asp Lys Asp Lys Glu Thr Asp Tyr Arg 
    530                 535                 540                 


Ala Thr Ile Leu Arg Tyr Gly Ser Lys Tyr Tyr Leu Ala Ile Met Asp 
545                 550                 555                 560 


Lys Lys Tyr Ala Lys Cys Leu Gln Lys Ile Asp Lys Asp Asp Val Asn 
                565                 570                 575     


Gly Asn Tyr Glu Lys Ile Asn Tyr Lys Leu Leu Pro Gly Pro Asn Lys 
            580                 585                 590         


Met Leu Pro Lys Val Phe Phe Ser Lys Lys Trp Met Ala Tyr Tyr Asn 
        595                 600                 605             


Pro Ser Glu Asp Ile Gln Lys Ile Tyr Lys Asn Gly Thr Phe Lys Lys 
    610                 615                 620                 


Gly Asp Met Phe Asn Leu Asn Asp Cys His Lys Leu Ile Asp Phe Phe 
625                 630                 635                 640 


Lys Asp Ser Ile Ser Arg Tyr Pro Lys Trp Ser Asn Ala Tyr Asp Phe 
                645                 650                 655     


Asn Phe Ser Glu Thr Glu Lys Tyr Lys Asp Ile Ala Gly Phe Tyr Arg 
            660                 665                 670         


Glu Val Glu Glu Gln Gly Tyr Lys Val Ser Phe Glu Ser Ala Ser Lys 
        675                 680                 685             


Lys Glu Val Asp Lys Leu Val Glu Glu Gly Lys Leu Tyr Met Phe Gln 
    690                 695                 700                 


Ile Tyr Asn Lys Asp Phe Ser Asp Lys Ser His Gly Thr Pro Asn Leu 
705                 710                 715                 720 


His Thr Met Tyr Phe Lys Leu Leu Phe Asp Glu Asn Asn His Gly Gln 
                725                 730                 735     


Ile Arg Leu Ser Gly Gly Ala Glu Leu Phe Met Arg Arg Ala Ser Leu 
            740                 745                 750         


Lys Lys Glu Glu Leu Val Val His Pro Ala Asn Ser Pro Ile Ala Asn 
        755                 760                 765             


Lys Asn Pro Asp Asn Pro Lys Lys Thr Thr Thr Leu Ser Tyr Asp Val 
    770                 775                 780                 


Tyr Lys Asp Lys Arg Phe Ser Glu Asp Gln Tyr Glu Leu His Ile Pro 
785                 790                 795                 800 


Ile Ala Ile Asn Lys Cys Pro Lys Asn Ile Phe Lys Ile Asn Thr Glu 
                805                 810                 815     


Val Arg Val Leu Leu Lys His Asp Asp Asn Pro Tyr Val Ile Gly Ile 
            820                 825                 830         


Asp Arg Gly Glu Arg Asn Leu Leu Tyr Ile Val Val Val Asp Gly Lys 
        835                 840                 845             


Gly Asn Ile Val Glu Gln Tyr Ser Leu Asn Glu Ile Ile Asn Asn Phe 
    850                 855                 860                 


Asn Gly Ile Arg Ile Lys Thr Asp Tyr His Ser Leu Leu Asp Lys Lys 
865                 870                 875                 880 


Glu Lys Glu Arg Phe Glu Ala Arg Gln Asn Trp Thr Ser Ile Glu Asn 
                885                 890                 895     


Ile Lys Glu Leu Lys Ala Gly Tyr Ile Ser Gln Val Val His Lys Ile 
            900                 905                 910         


Cys Glu Leu Val Glu Lys Tyr Asp Ala Val Ile Ala Leu Glu Asp Leu 
        915                 920                 925             


Asn Ser Gly Phe Lys Asn Ser Arg Val Lys Val Glu Lys Gln Val Tyr 
    930                 935                 940                 


Gln Lys Phe Glu Lys Met Leu Ile Asp Lys Leu Asn Tyr Met Val Asp 
945                 950                 955                 960 


Lys Lys Ser Asn Pro Cys Ala Thr Gly Gly Ala Leu Lys Gly Tyr Gln 
                965                 970                 975     


Ile Thr Asn Lys Phe Glu Ser Phe Lys Ser Met Ser Thr Gln Asn Gly 
            980                 985                 990         


Phe Ile Phe Tyr Ile Pro Ala Trp  Leu Thr Ser Lys Ile  Asp Pro Ser 
        995                 1000                 1005             


Thr Gly  Phe Val Asn Leu Leu  Lys Thr Lys Tyr Thr  Ser Ile Ala 
    1010                 1015                 1020             


Asp Ser  Lys Lys Phe Ile Ser  Ser Phe Asp Arg Ile  Met Tyr Val 
    1025                 1030                 1035             


Pro Glu  Glu Asp Leu Phe Glu  Phe Ala Leu Asp Tyr  Lys Asn Phe 
    1040                 1045                 1050             


Ser Arg  Thr Asp Ala Asp Tyr  Ile Lys Lys Trp Lys  Leu Tyr Ser 
    1055                 1060                 1065             


Tyr Gly  Asn Arg Ile Arg Ile  Phe Ala Ala Ala Lys  Lys Asn Asn 
    1070                 1075                 1080             


Val Phe  Ala Trp Glu Glu Val  Cys Leu Thr Ser Ala  Tyr Lys Glu 
    1085                 1090                 1095             


Leu Phe  Asn Lys Tyr Gly Ile  Asn Tyr Gln Gln Gly  Asp Ile Arg 
    1100                 1105                 1110             


Ala Leu  Leu Cys Glu Gln Ser  Asp Lys Ala Phe Tyr  Ser Ser Phe 
    1115                 1120                 1125             


Met Ala  Leu Met Ser Leu Met  Leu Gln Met Arg Asn  Ser Ile Thr 
    1130                 1135                 1140             


Gly Arg  Thr Asp Val Asp Phe  Leu Ile Ser Pro Val  Lys Asn Ser 
    1145                 1150                 1155             


Asp Gly  Ile Phe Tyr Asp Ser  Arg Asn Tyr Glu Ala  Gln Glu Asn 
    1160                 1165                 1170             


Ala Ile  Leu Pro Lys Asn Ala  Asp Ala Asn Gly Ala  Tyr Asn Ile 
    1175                 1180                 1185             


Ala Arg  Lys Val Leu Trp Ala  Ile Gly Gln Phe Lys  Lys Ala Glu 
    1190                 1195                 1200             


Asp Glu  Lys Leu Asp Lys Val  Lys Ile Ala Ile Ser  Asn Lys Glu 
    1205                 1210                 1215             


Trp Leu  Glu Tyr Ala Gln Thr  Ser Val Lys 
    1220                 1225             


<210>  1351
<211>  1300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1351

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asp Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Val Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Asp Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Leu Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Glu Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Arg Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Asn Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Glu Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu His Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Asp Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  1352
<211>  356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1352

Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile Glu Lys Asp Arg 
1               5                   10                  15      


Glu Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn Ile Lys Glu Met 
            20                  25                  30          


Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile Ala Lys Leu Val 
        35                  40                  45              


Ile Gly Tyr Asn Ala Ile Val Val Phe Glu Asp Leu Asn Phe Gly Phe 
    50                  55                  60                  


Lys Arg Gly Arg Phe Lys Val Glu Lys Gln Val Tyr Gln Lys Leu Glu 
65                  70                  75                  80  


Lys Met Leu Ile Glu Lys Leu Asn Tyr Leu Val Phe Lys Asp Asn Glu 
                85                  90                  95      


Phe Asp Lys Ala Gly Gly Val Leu Arg Ala Tyr Gln Leu Thr Ala Pro 
            100                 105                 110         


Phe Glu Thr Phe Lys Lys Met Gly Lys Gln Thr Gly Ile Ile Tyr Tyr 
        115                 120                 125             


Val Pro Ala Asp Phe Thr Ser Lys Ile Cys Pro Val Thr Gly Phe Val 
    130                 135                 140                 


Asn Gln Leu Tyr Pro Lys Tyr Glu Ser Val Ser Lys Ser Gln Glu Phe 
145                 150                 155                 160 


Phe Ser Lys Phe Asp Lys Ile Cys Tyr Asn Leu Asp Lys Gly Tyr Phe 
                165                 170                 175     


Glu Phe Ser Phe Asp Tyr Lys Asn Phe Gly Asp Lys Ala Ala Lys Gly 
            180                 185                 190         


Lys Trp Thr Ile Ala Ser Phe Gly Ser Arg Leu Ile Asn Phe Arg Asn 
        195                 200                 205             


Ser Asp Lys Asn His Asn Trp Asp Thr Arg Glu Val Tyr Pro Thr Lys 
    210                 215                 220                 


Glu Leu Glu Lys Leu Leu Lys Asp Tyr Ser Ile Glu Tyr Gly His Gly 
225                 230                 235                 240 


Glu Cys Ile Lys Ala Ala Ile Tyr Ala Glu Asn Asp Lys Lys Phe Phe 
                245                 250                 255     


Ala Lys Leu Thr Ser Ile Leu Asn Ser Ile Leu Gln Met Arg Asn Ser 
            260                 265                 270         


Lys Thr Gly Thr Glu Leu Asp Tyr Leu Ile Ser Pro Val Ala Asp Val 
        275                 280                 285             


Asn Gly Asn Phe Phe Asp Ser Arg His Ala Pro Lys Asn Met Pro Gln 
    290                 295                 300                 


Asp Ala Asp Ala Asn Gly Ala Tyr His Ile Gly Leu Lys Gly Leu Met 
305                 310                 315                 320 


Leu Leu Tyr Arg Ile Lys Asn Asn Gln Asp Gly Lys Lys Leu Asn Leu 
                325                 330                 335     


Val Ile Lys Asn Glu Glu Tyr Phe Glu Phe Val Gln Asn Arg Asn Lys 
            340                 345                 350         


Ser Ser Lys Ile 
        355     


<210>  1353
<211>  1263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1353

Met Glu Asp Tyr Ser Gly Phe Val Asn Ile Tyr Ser Ile Gln Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Lys Pro Val Gly Lys Thr Leu Glu His Ile Glu 
            20                  25                  30          


Lys Lys Gly Phe Leu Lys Lys Asp Lys Ile Arg Ala Glu Asp Tyr Lys 
        35                  40                  45              


Ala Val Lys Lys Ile Ile Asp Lys Tyr His Arg Ala Tyr Ile Glu Glu 
    50                  55                  60                  


Val Phe Asp Ser Val Leu His Gln Lys Lys Lys Lys Asp Lys Thr Arg 
65                  70                  75                  80  


Phe Ser Thr Gln Phe Ile Lys Glu Ile Lys Glu Phe Ser Glu Leu Tyr 
                85                  90                  95      


Tyr Lys Thr Glu Lys Asn Ile Pro Asp Lys Glu Arg Leu Glu Ala Leu 
            100                 105                 110         


Ser Glu Lys Leu Arg Lys Met Leu Val Gly Ala Phe Lys Gly Glu Phe 
        115                 120                 125             


Ser Glu Glu Val Ala Glu Lys Tyr Lys Asn Leu Phe Ser Lys Glu Leu 
    130                 135                 140                 


Ile Arg Asn Glu Ile Glu Lys Phe Cys Glu Thr Asp Glu Glu Arg Lys 
145                 150                 155                 160 


Gln Val Ser Asn Phe Lys Ser Phe Thr Thr Tyr Phe Thr Gly Phe His 
                165                 170                 175     


Ser Asn Arg Gln Asn Ile Tyr Ser Asp Glu Lys Lys Ser Thr Ala Ile 
            180                 185                 190         


Gly Tyr Arg Ile Ile His Gln Asn Leu Pro Lys Phe Leu Asp Asn Leu 
        195                 200                 205             


Lys Ile Ile Glu Ser Ile Gln Arg Arg Phe Lys Asp Phe Pro Trp Ser 
    210                 215                 220                 


Asp Leu Lys Lys Asn Leu Lys Lys Ile Asp Lys Asn Ile Lys Leu Thr 
225                 230                 235                 240 


Glu Tyr Phe Ser Ile Asp Gly Phe Val Asn Val Leu Asn Gln Lys Gly 
                245                 250                 255     


Ile Asp Ala Tyr Asn Thr Ile Leu Gly Gly Lys Ser Glu Glu Ser Gly 
            260                 265                 270         


Glu Lys Ile Gln Gly Leu Asn Glu Tyr Ile Asn Leu Tyr Arg Gln Lys 
        275                 280                 285             


Asn Asn Ile Asp Arg Lys Asn Leu Pro Asn Val Lys Ile Leu Phe Lys 
    290                 295                 300                 


Gln Ile Leu Gly Asp Arg Glu Thr Lys Ser Phe Ile Pro Glu Ala Phe 
305                 310                 315                 320 


Pro Asp Asp Gln Ser Val Leu Asn Ser Ile Thr Glu Phe Ala Lys Tyr 
                325                 330                 335     


Leu Lys Leu Asp Lys Lys Lys Lys Ser Ile Ile Ala Glu Leu Lys Lys 
            340                 345                 350         


Phe Leu Ser Ser Phe Asn Arg Tyr Glu Leu Asp Gly Ile Tyr Leu Ala 
        355                 360                 365             


Asn Asp Asn Ser Leu Ala Ser Ile Ser Thr Phe Leu Phe Asp Asp Trp 
    370                 375                 380                 


Ser Phe Ile Lys Lys Ser Val Ser Phe Lys Tyr Asp Glu Ser Val Gly 
385                 390                 395                 400 


Asp Pro Lys Lys Lys Ile Lys Ser Pro Leu Lys Tyr Glu Lys Glu Lys 
                405                 410                 415     


Glu Lys Trp Leu Lys Gln Lys Tyr Tyr Thr Ile Ser Phe Leu Asn Asp 
            420                 425                 430         


Ala Ile Glu Ser Tyr Ser Lys Ser Gln Asp Glu Lys Arg Val Lys Ile 
        435                 440                 445             


Arg Leu Glu Ala Tyr Phe Ala Glu Phe Lys Ser Lys Asp Asp Ala Lys 
    450                 455                 460                 


Lys Gln Phe Asp Leu Leu Glu Arg Ile Glu Glu Ala Tyr Ala Ile Val 
465                 470                 475                 480 


Glu Pro Leu Leu Gly Ala Glu Tyr Pro Arg Asp Arg Asn Leu Lys Ala 
                485                 490                 495     


Asp Lys Lys Glu Val Gly Lys Ile Lys Asp Phe Leu Asp Ser Ile Lys 
            500                 505                 510         


Ser Leu Gln Phe Phe Leu Lys Pro Leu Leu Ser Ala Glu Ile Phe Asp 
        515                 520                 525             


Glu Lys Asp Leu Gly Phe Tyr Asn Gln Leu Glu Gly Tyr Tyr Glu Glu 
    530                 535                 540                 


Ile Asp Ser Ile Gly His Leu Tyr Asn Lys Val Arg Asn Tyr Leu Thr 
545                 550                 555                 560 


Gly Lys Ile Tyr Ser Lys Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser 
                565                 570                 575     


Thr Leu Leu Lys Gly Trp Asp Glu Asn Arg Glu Val Ala Asn Leu Cys 
            580                 585                 590         


Val Ile Phe Arg Glu Asp Gln Lys Tyr Tyr Leu Gly Val Met Asp Lys 
        595                 600                 605             


Glu Asn Asn Thr Ile Leu Ser Asp Ile Pro Lys Val Lys Pro Asn Glu 
    610                 615                 620                 


Leu Phe Tyr Glu Lys Met Val Tyr Lys Leu Ile Pro Thr Pro His Met 
625                 630                 635                 640 


Gln Leu Pro Arg Ile Ile Phe Ser Ser Asp Asn Leu Ser Ile Tyr Asn 
                645                 650                 655     


Pro Ser Lys Ser Ile Leu Lys Ile Arg Glu Ala Lys Ser Phe Lys Glu 
            660                 665                 670         


Gly Lys Asn Phe Lys Leu Lys Asp Cys His Lys Phe Ile Asp Phe Tyr 
        675                 680                 685             


Lys Glu Ser Ile Ser Lys Asn Glu Asp Trp Ser Arg Phe Asp Phe Lys 
    690                 695                 700                 


Phe Ser Lys Thr Ser Ser Tyr Glu Asn Ile Ser Glu Phe Tyr Arg Glu 
705                 710                 715                 720 


Val Glu Arg Gln Gly Tyr Asn Leu Asp Phe Lys Lys Val Ser Lys Phe 
                725                 730                 735     


Tyr Ile Asp Ser Leu Val Glu Asp Gly Lys Leu Tyr Leu Phe Gln Ile 
            740                 745                 750         


Tyr Asn Lys Asp Phe Ser Ile Phe Ser Lys Gly Lys Pro Asn Leu His 
        755                 760                 765             


Thr Ile Tyr Phe Arg Ser Leu Phe Ser Lys Glu Asn Leu Lys Asp Val 
    770                 775                 780                 


Cys Leu Lys Leu Asn Gly Glu Ala Glu Met Phe Phe Arg Lys Lys Ser 
785                 790                 795                 800 


Ile Asn Tyr Asp Glu Lys Lys Lys Arg Glu Gly His His Pro Glu Leu 
                805                 810                 815     


Phe Glu Lys Leu Lys Tyr Pro Ile Leu Lys Asp Lys Arg Tyr Ser Glu 
            820                 825                 830         


Asp Lys Phe Gln Phe His Leu Pro Ile Ser Leu Asn Phe Lys Ser Lys 
        835                 840                 845             


Glu Arg Leu Asn Phe Asn Leu Lys Val Asn Glu Phe Leu Lys Arg Asn 
    850                 855                 860                 


Lys Asp Ile Asn Ile Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Leu 
865                 870                 875                 880 


Tyr Leu Val Met Ile Asn Gln Lys Gly Glu Ile Leu Lys Gln Thr Leu 
                885                 890                 895     


Leu Asp Ser Met Gln Ser Gly Lys Gly Arg Pro Glu Ile Asn Tyr Lys 
            900                 905                 910         


Glu Lys Leu Gln Glu Lys Glu Ile Glu Arg Asp Lys Ala Arg Lys Ser 
        915                 920                 925             


Trp Gly Thr Val Glu Asn Ile Lys Glu Leu Lys Glu Gly Tyr Leu Ser 
    930                 935                 940                 


Ile Val Ile His Gln Ile Ser Lys Leu Met Val Glu Asn Asn Ala Ile 
945                 950                 955                 960 


Val Val Leu Glu Asp Leu Asn Ile Gly Phe Lys Arg Gly Arg Gln Lys 
                965                 970                 975     


Val Glu Arg Gln Val Tyr Gln Lys Phe Glu Lys Met Leu Ile Asp Lys 
            980                 985                 990         


Leu Asn Phe Leu Val Phe Lys Glu  Asn Lys Pro Thr Glu  Pro Gly Gly 
        995                 1000                 1005             


Val Leu  Lys Ala Tyr Gln Leu  Thr Asp Glu Phe Gln  Ser Phe Glu 
    1010                 1015                 1020             


Lys Leu  Ser Lys Gln Thr Gly  Phe Leu Phe Tyr Val  Pro Ser Trp 
    1025                 1030                 1035             


Asn Thr  Ser Lys Ile Asp Pro  Arg Thr Gly Phe Ile  Asp Phe Leu 
    1040                 1045                 1050             


His Pro  Ala Tyr Glu Asn Ile  Glu Lys Ala Lys Gln  Trp Ile Asn 
    1055                 1060                 1065             


Lys Phe  Asp Ser Ile Arg Phe  Asn Ser Lys Met Asp  Trp Phe Glu 
    1070                 1075                 1080             


Phe Thr  Ala Asp Thr Arg Lys  Phe Ser Glu Asn Leu  Met Leu Gly 
    1085                 1090                 1095             


Lys Asn  Arg Val Trp Val Ile  Cys Thr Thr Asn Val  Glu Arg Tyr 
    1100                 1105                 1110             


Phe Thr  Ser Lys Thr Ala Asn  Ser Ser Ile Gln Tyr  Asn Ser Ile 
    1115                 1120                 1125             


Gln Ile  Thr Glu Lys Leu Lys  Glu Leu Phe Val Asp  Ile Pro Phe 
    1130                 1135                 1140             


Ser Asn  Gly Gln Asp Leu Lys  Pro Glu Ile Leu Arg  Lys Asn Asp 
    1145                 1150                 1155             


Ala Val  Phe Phe Lys Ser Leu  Leu Phe Tyr Ile Lys  Thr Thr Leu 
    1160                 1165                 1170             


Ser Leu  Arg Gln Asn Asn Gly  Lys Lys Gly Glu Glu  Glu Lys Asp 
    1175                 1180                 1185             


Phe Ile  Leu Ser Pro Val Val  Asp Ser Lys Gly Arg  Phe Phe Asn 
    1190                 1195                 1200             


Ser Leu  Glu Ala Ser Asp Asp  Glu Pro Lys Asp Ala  Asp Ala Asn 
    1205                 1210                 1215             


Gly Ala  Tyr His Ile Ala Leu  Lys Gly Leu Met Asn  Leu Leu Val 
    1220                 1225                 1230             


Leu Asn  Glu Thr Lys Glu Glu  Asn Leu Ser Arg Pro  Lys Trp Lys 
    1235                 1240                 1245             


Ile Lys  Asn Lys Asp Trp Leu  Glu Phe Val Trp Glu  Arg Asn Arg 
    1250                 1255                 1260             


<210>  1354
<211>  939
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1354

Met Asn Leu Tyr Ser Asn Leu Thr Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Glu Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys His Ile Ser Arg Tyr 
            100                 105                 110         


Ile Asn Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asp Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Ile Glu Asn Lys Ala Lys Tyr Glu Asn Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Leu Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Val Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Asp Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys Tyr Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Phe Ala 
465                 470                 475                 480 


Ser Phe Ala Asp Ile Pro Val Leu Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asn Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Thr Ser Ala Glu Val Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Thr Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Asp Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Ala Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Val Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Asn Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Leu Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys His Ser Ile Ser Arg His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Lys Lys Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Leu Val Asp Glu Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Val Tyr Ser Lys Gly Lys 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Ile Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Cys Lys Asn 
    930                 935                 


<210>  1355
<211>  1264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1355

Met Leu Phe Gln Glu Phe Thr His Leu Tyr Pro Leu Ser Lys Thr Val 
1               5                   10                  15      


Arg Phe Glu Leu Lys Pro Ile Gly Lys Thr Leu Glu His Ile His Ala 
            20                  25                  30          


Lys Asp Phe Leu Ser Gln Asp Glu Thr Met Ala Asp Met Tyr Gln Lys 
        35                  40                  45              


Val Lys Ala Ile Leu Asp Asp Tyr His Arg Asp Phe Ile Ala Asp Met 
    50                  55                  60                  


Met Ser Gly Val Val Leu Thr Lys Leu Ala Glu Phe Tyr Glu Val Tyr 
65                  70                  75                  80  


Leu Ala Leu Arg Lys Thr Pro Lys Asp Asp Ser Leu Gln Lys Gln Leu 
                85                  90                  95      


Thr Glu Ile Gln Thr Ala Leu Arg Lys Glu Ile Val Lys Pro Ile Gly 
            100                 105                 110         


Ser Gly Gly Lys Tyr Lys Ala Gly Tyr Asp Arg Leu Phe Gly Ala Lys 
        115                 120                 125             


Leu Phe Lys Asp Gly Lys Glu Leu Gly Asp Leu Ala Lys Phe Val Ile 
    130                 135                 140                 


Thr Gln Glu Gly Glu Ser Ser Pro Lys Leu Ala His Ile Ala His Phe 
145                 150                 155                 160 


Glu Lys Phe Ser Thr Tyr Phe Thr Gly Phe His Asp Asn Arg Lys Asn 
                165                 170                 175     


Met Tyr Ser Asp Glu Asp Lys His Thr Ala Ile Ala Tyr Arg Leu Ile 
            180                 185                 190         


His Glu Asn Leu Pro Arg Phe Ile Asp Asn Leu Gln Ile Leu Ala Thr 
        195                 200                 205             


Ile Lys Gln Lys His Ser Ala Leu Tyr Asp Gln Ile Val Asn Glu Leu 
    210                 215                 220                 


Asn Ala Asn Gly Leu Asp Val Ser Leu Ala Ser His Leu Asp Gly Tyr 
225                 230                 235                 240 


His Lys Leu Leu Thr Gln Glu Gly Ile Thr Ala Tyr Asn Thr Leu Leu 
                245                 250                 255     


Gly Gly Ile Ser Gly Glu Ala Gly Ser Arg Lys Ile Gln Gly Ile Asn 
            260                 265                 270         


Glu Leu Ile Asn Ile His His Asn Gln His Cys Gln Lys Ser Glu Arg 
        275                 280                 285             


Ile Ala Lys Leu Arg Pro Leu His Lys Gln Ile Leu Ser Asp Gly Met 
    290                 295                 300                 


Gly Val Ser Phe Leu Pro Ser Lys Phe Ala Asp Asp Ser Glu Val Cys 
305                 310                 315                 320 


Gln Ala Val Asn Glu Phe Tyr Arg His Tyr Ala His Val Phe Ala Lys 
                325                 330                 335     


Val Gln Ser Leu Phe Asp Arg Phe Asp Asp Tyr Arg Lys Asp Gly Ile 
            340                 345                 350         


Tyr Val Glu His Lys Asn Leu Asn Glu Leu Ser Lys Gln Ala Phe Gly 
        355                 360                 365             


Asp Phe Ala Leu Leu Gly Arg Val Leu Asp Gly Tyr Tyr Val Asp Val 
    370                 375                 380                 


Val Asn Pro Glu Phe Asn Glu Arg Phe Ala Lys Ala Lys Thr Asp Asn 
385                 390                 395                 400 


Ala Lys Glu Lys Leu Thr Lys Glu Lys Asp Lys Phe Ile Lys Gly Val 
                405                 410                 415     


His Ser Leu Ala Ser Leu Glu Gln Ala Ile Glu His Tyr Ile Ala Gly 
            420                 425                 430         


His Asp Asp Glu Ser Val Gln Ala Gly Lys Leu Gly Gln Tyr Phe Lys 
        435                 440                 445             


His Gly Leu Ala Gly Val Asp Asn Pro Ile Gln Lys Ile His Asn Ser 
    450                 455                 460                 


His Ser Thr Ile Lys Gly Phe Leu Glu Arg Glu Arg Pro Ala Gly Glu 
465                 470                 475                 480 


Arg Ala Leu Pro Lys Ile Lys Ser Asp Lys Ser Pro Glu Met Thr Gln 
                485                 490                 495     


Leu Arg Gln Leu Lys Glu Leu Leu Asp Asn Ala Leu Asn Val Val His 
            500                 505                 510         


Phe Ala Lys Leu Leu Thr Thr Lys Thr Thr Leu Asp Asn Gln Asp Gly 
        515                 520                 525             


Asn Phe Tyr Gly Glu Phe Gly Ala Leu Tyr Asp Glu Leu Ala Lys Ile 
    530                 535                 540                 


Ala Thr Leu Tyr Asn Lys Val Arg Asp Tyr Leu Ser Gln Lys Pro Phe 
545                 550                 555                 560 


Ser Thr Glu Lys Tyr Lys Leu Asn Phe Gly Asn Pro Thr Leu Leu Asn 
                565                 570                 575     


Gly Trp Asp Leu Asn Lys Glu Lys Asp Asn Phe Gly Val Ile Leu Gln 
            580                 585                 590         


Lys Asp Gly Cys Tyr Tyr Leu Ala Leu Leu Asp Lys Ala His Lys Lys 
        595                 600                 605             


Val Phe Asp Asn Ala Pro Asn Ala Gly Lys Ser Val Tyr Gln Lys Met 
    610                 615                 620                 


Val Tyr Lys Leu Leu Pro Gly Pro Asn Lys Met Leu Pro Lys Val Phe 
625                 630                 635                 640 


Phe Ala Lys Ser Asn Leu Asp Tyr Tyr Asn Pro Ser Ala Glu Leu Leu 
                645                 650                 655     


Asp Lys Tyr Ala Gln Gly Thr His Lys Lys Gly Asn Asn Phe Asn Leu 
            660                 665                 670         


Lys Asp Cys His Ala Leu Ile Asp Phe Phe Lys Ala Ser Ile Asn Lys 
        675                 680                 685             


His Pro Glu Trp Gln His Phe Gly Phe Glu Phe Ser Pro Thr Ser Ser 
    690                 695                 700                 


Tyr Gln Asp Leu Ser Asp Phe Tyr Arg Glu Val Glu Pro Gln Gly Tyr 
705                 710                 715                 720 


Gln Val Lys Phe Val Asp Ile Asn Ala Glu Tyr Ile Asp Glu Leu Val 
                725                 730                 735     


Glu Gln Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser 
            740                 745                 750         


Pro Lys Ala His Gly Lys Pro Asn Leu His Thr Leu Tyr Phe Lys Ala 
        755                 760                 765             


Leu Phe Ser Glu Asn Asn Leu Ala Asn Pro Ile Tyr Lys Leu Asn Gly 
    770                 775                 780                 


Glu Ala Glu Ile Phe Tyr Arg Lys Ala Ser Leu Asp Met Asn Glu Thr 
785                 790                 795                 800 


Thr Ile His Arg Ala Gly Glu Val Leu Glu Asn Lys Asn Pro Asp Asn 
                805                 810                 815     


Pro Lys Lys Arg Gln Phe Val Tyr Asp Ile Ile Lys Asp Lys Arg Tyr 
            820                 825                 830         


Thr Gln Asp Lys Phe Met Leu His Val Pro Ile Thr Met Asn Phe Gly 
        835                 840                 845             


Val Gln Gly Met Thr Ile Lys Glu Phe Asn Lys Lys Val Asn Gln Ser 
    850                 855                 860                 


Ile Gln Gln Tyr Asn Asp Val Asn Val Ile Gly Ile Asp Arg Gly Glu 
865                 870                 875                 880 


Arg His Leu Leu Tyr Leu Thr Val Ile Asn Ser Lys Gly Glu Ile Leu 
                885                 890                 895     


Glu Gln Arg Ser Leu Asn Asp Ile Ile Thr Thr Ser Ala Asn Gly Thr 
            900                 905                 910         


Gln Met Thr Thr Pro Tyr His Lys Ile Leu Asp Lys Arg Glu Ile Glu 
        915                 920                 925             


Arg Leu Asn Ala Arg Val Gly Trp Gly Glu Ile Glu Thr Ile Lys Glu 
    930                 935                 940                 


Leu Lys Ser Gly Tyr Leu Ser His Val Val His Gln Ile Ser Gln Leu 
945                 950                 955                 960 


Met Leu Lys Tyr Asn Ala Ile Val Val Leu Glu Asp Leu Asn Phe Gly 
                965                 970                 975     


Phe Lys Arg Gly Arg Phe Lys Val Glu Lys Gln Ile Tyr Gln Asn Phe 
            980                 985                 990         


Glu Asn Ala Leu Ile Lys Lys Leu  Asn His Leu Ala Leu  Lys Asp Lys 
        995                 1000                 1005             


Ala Asp  Asp Glu Ile Gly Ser  Tyr Lys Asn Ala Leu  Gln Leu Thr 
    1010                 1015                 1020             


Asn Asn  Phe Thr Asp Leu Lys  Ser Ile Gly Lys Gln  Thr Gly Phe 
    1025                 1030                 1035             


Leu Phe  Tyr Val Pro Ala Trp  Asn Thr Ser Lys Ile  Asp Pro Ala 
    1040                 1045                 1050             


Thr Gly  Phe Val Asp Leu Leu  Lys Pro Arg Tyr Glu  Asn Ile Ala 
    1055                 1060                 1065             


Gln Ser  Gln Ala Phe Phe Gly  Lys Phe Asp Lys Ile  Phe Tyr Asn 
    1070                 1075                 1080             


Ala Asp  Lys Gly Tyr Phe Glu  Phe Tyr Val Asp Tyr  Ala Lys Phe 
    1085                 1090                 1095             


Thr Asp  Lys Ala Lys Asn Ser  Arg Gln Thr Trp Val  Ile Cys Ser 
    1100                 1105                 1110             


His Gly  Asp Lys Arg Tyr Val  Tyr Asp Lys Thr Ala  Asn Gln Asn 
    1115                 1120                 1125             


Lys Gly  Ala Thr Ile Gly Ile  Asn Val Asn Asp Glu  Leu Lys Ser 
    1130                 1135                 1140             


Leu Phe  Ala Arg Tyr His Ile  Asn Asp Lys Gln Pro  Asn Leu Val 
    1145                 1150                 1155             


Met Asp  Ile Cys Gln Asn Asn  Asp Lys Glu Phe His  Lys Ser Leu 
    1160                 1165                 1170             


Met Tyr  Leu Leu Lys Ala Leu  Leu Ala Leu Arg Tyr  Ser Asn Ala 
    1175                 1180                 1185             


Ser Ser  Asp Glu Asp Phe Ile  Leu Ser Pro Val Ala  Asp Asp Lys 
    1190                 1195                 1200             


Gly Val  Phe Phe Asn Ser Ala  Leu Ala Asp Asp Thr  Gln Pro Gln 
    1205                 1210                 1215             


Asn Ala  Asp Ala Asn Gly Ala  Tyr His Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Trp Leu  Leu Asn Glu Leu Lys  Asn Ser Asn Asp Leu  Asp Lys Ile 
    1235                 1240                 1245             


Lys Leu  Ala Ile Asp Asn Gln  Thr Trp Leu Asn Phe  Ala Gln Asn 
    1250                 1255                 1260             


Arg 
    


<210>  1356
<211>  1300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1356

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asp Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Val Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Asp Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Leu Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Glu Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Arg Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Asn Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Glu Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu His Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Asp Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  1357
<211>  1251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1357

Met Asn His Met Lys Gln Phe Thr Asn Gln Phe Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Lys Glu Phe Ile Glu 
            20                  25                  30          


Ile Asn Gly Leu Ile Glu Lys Asp Asn Glu Arg Ala Val Ser Tyr Lys 
        35                  40                  45              


Lys Val Lys Lys Ile Ile Asp Glu Tyr His Lys Tyr Phe Ile Glu Met 
    50                  55                  60                  


Val Leu Cys Asp Phe Lys Leu His Gly Leu Glu Thr Tyr Glu Thr Ile 
65                  70                  75                  80  


Phe Asn Lys Lys Glu Lys Asp Asp Thr Asp Lys Lys Glu Phe Asp Asn 
                85                  90                  95      


Ile Arg Asn Ser Leu Arg Lys Gln Ile Ala Asp Ala Phe Ala Lys Asn 
            100                 105                 110         


Pro Asn Asp Glu Ile Lys Glu Arg Phe Lys Asn Leu Phe Ala Lys Glu 
        115                 120                 125             


Leu Ile Lys Gln Asp Leu Leu Asn Phe Val Asp Asp Glu Gln Lys Glu 
    130                 135                 140                 


Leu Val Asn Glu Phe Lys Asp Phe Thr Thr Tyr Phe Thr Gly Phe His 
145                 150                 155                 160 


Gln Asn Arg Arg Asn Met Tyr Val Ala Asp Glu Lys Ala Thr Ala Ile 
                165                 170                 175     


Ala Tyr Arg Leu Val Asn Glu Asn Leu Pro Lys Phe Ile Asp Asn Leu 
            180                 185                 190         


Lys Ile Tyr Glu Lys Ile Lys Lys Asp Ala Pro Glu Leu Ile Ser Asp 
        195                 200                 205             


Leu Asn Lys Thr Leu Val Glu Met Glu Glu Ile Val Gln Gly Lys Thr 
    210                 215                 220                 


Leu Asp Glu Ile Phe Ser Leu Ser Phe Phe Asn Gln Thr Leu Thr Gln 
225                 230                 235                 240 


Thr Gly Ile Glu Leu Tyr Asn Ile Val Ile Gly Gly Arg Thr Ala Asp 
                245                 250                 255     


Glu Gly Lys Thr Lys Ile Lys Gly Leu Asn Glu Tyr Ile Asn Thr Asp 
            260                 265                 270         


Tyr Asn Gln Lys Gln Thr Asp Lys Lys Lys Lys Gln Ala Lys Phe Lys 
        275                 280                 285             


Gln Leu Tyr Lys Gln Ile Leu Ser Asp Arg His Ser Val Ser Phe Val 
    290                 295                 300                 


Ala Glu Thr Phe Glu Thr Asp Ala Gln Leu Leu Glu Asn Ile Glu Gln 
305                 310                 315                 320 


Phe Tyr Ser Ser Val Leu Cys Asn Tyr Glu Asp Asp Gly His Thr Thr 
                325                 330                 335     


Asn Ile Phe Glu Ala Ile Lys Asn Leu Ile Ile Gly Leu Lys Thr Phe 
            340                 345                 350         


Asp Leu Ser Lys Ile Tyr Leu Arg Asn Asp Thr Ser Leu Thr Asp Ile 
        355                 360                 365             


Ser Gln Lys Leu Phe Gly Asp Trp Ser Ile Ile Ser Ser Ala Leu Asn 
    370                 375                 380                 


Asp Tyr Tyr Glu Lys Gln Asn Pro Ile Ser Ser Lys Glu Lys Gln Glu 
385                 390                 395                 400 


Lys Tyr Asp Glu Arg Lys Ala Lys Trp Leu Lys Gln Asp Phe Asn Ile 
                405                 410                 415     


Glu Thr Ile Gln Thr Ala Leu Asn Glu Cys Asp Ser Glu Ile Ile Lys 
            420                 425                 430         


Glu Lys Asn Asn Lys Asn Ile Val Ser Glu Tyr Phe Ala Lys Leu Gly 
        435                 440                 445             


Leu Asp Lys Asp Asn Lys Ile Asp Leu Leu Gln Lys Ile His His Asn 
    450                 455                 460                 


Tyr Val Val Ile Lys Asp Leu Leu Asn Glu Pro Tyr Pro Glu Asn Ile 
465                 470                 475                 480 


Lys Leu Gly Asn Gln Lys Glu Gln Val Ser Gln Ile Lys Asp Phe Leu 
                485                 490                 495     


Asp Ser Ile Leu Asn Leu Ile His Phe Leu Lys Pro Leu Ser Leu Lys 
            500                 505                 510         


Asp Lys Asp Lys Glu Lys Asp Glu Leu Phe Tyr Ser Leu Phe Thr Ala 
        515                 520                 525             


Leu Phe Glu His Leu Ser Gln Thr Ile Ser Ile Tyr Asn Lys Val Arg 
    530                 535                 540                 


Asn Tyr Leu Thr Gln Lys Ala Tyr Ser Thr Glu Lys Ile Lys Leu Asn 
545                 550                 555                 560 


Phe Glu Asn Ser Thr Leu Leu Asn Gly Trp Asp Val Asn Lys Glu Pro 
                565                 570                 575     


Val Asn Thr Ser Val Ile Phe Arg Lys Asn Gly Leu Phe Tyr Leu Gly 
            580                 585                 590         


Ile Met Ser Lys Ser Asn Asn Arg Ile Phe Glu Arg Asn Val Pro Val 
        595                 600                 605             


Cys Lys Asn Glu Glu Thr Ala Phe Glu Lys Met Asn Tyr Lys Leu Leu 
    610                 615                 620                 


Pro Gly Ala Asn Lys Met Leu Pro Lys Val Phe Leu Ser Ala Lys Gly 
625                 630                 635                 640 


Ile Glu Ser Phe Gln Pro Ser Ala Glu Ile Gln Ser Lys Tyr Gln Lys 
                645                 650                 655     


Glu Thr His Lys Lys Gly Asp Ala Phe Val Arg Lys Asp Met Glu Asn 
            660                 665                 670         


Leu Ile Asp Phe Phe Lys Gln Ser Ile Ala Lys His Thr Asp Trp Lys 
        675                 680                 685             


His Phe Asn His Gln Phe Ser Lys Thr Glu Thr Tyr Asn Asp Leu Ser 
    690                 695                 700                 


Glu Phe Tyr Lys Glu Val Glu Lys Gln Gly Tyr Lys Leu Thr Phe Thr 
705                 710                 715                 720 


Lys Leu Asp Glu Thr Tyr Ile Asn Gln Leu Val Asp Glu Gly Lys Leu 
                725                 730                 735     


Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Pro Phe Ser Lys Gly 
            740                 745                 750         


Lys Pro Asn Met His Thr Leu Tyr Trp Lys Met Leu Phe Asp Glu Gln 
        755                 760                 765             


Asn Leu Gln Asn Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Val Phe 
    770                 775                 780                 


Phe Arg Gln Ser Ser Ile Lys Gln Thr Asp Arg Ile Ile His Lys Ala 
785                 790                 795                 800 


Asn Gln Ala Ile Asp Asn Lys Asn Pro Leu Asn Asn Lys Lys Gln Ser 
                805                 810                 815     


Ser Phe Asn Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Leu Asp Lys 
            820                 825                 830         


Phe Gln Phe His Val Pro Ile Thr Leu Asn Phe Lys Ala Glu Gly Asn 
        835                 840                 845             


Glu Tyr Leu Asn Thr Lys Val Asn Glu Tyr Leu Lys Ser Asn Ser Asp 
    850                 855                 860                 


Val Lys Ile Ile Gly Leu Asp Arg Gly Glu Arg His Leu Ile Tyr Leu 
865                 870                 875                 880 


Thr Leu Ile Asn Gln Lys Gly Glu Leu Leu Lys Gln Gln Ser Leu Asn 
                885                 890                 895     


Val Ile Ala Thr Ser Gln Glu His Glu Thr Asp Tyr Lys Asn Leu Leu 
            900                 905                 910         


Val Asn Lys Glu Asn Glu Arg Ala Asn Ala Arg Gln Asp Trp Lys Thr 
        915                 920                 925             


Ile Glu Thr Ile Lys Glu Leu Lys Glu Gly Tyr Leu Ser Gln Val Val 
    930                 935                 940                 


His Gln Ile Ala Thr Met Met Val Asp Glu Asn Ala Ile Val Val Met 
945                 950                 955                 960 


Glu Asp Leu Asn Ala Gly Phe Met Arg Gly Arg Gln Lys Val Glu Arg 
                965                 970                 975     


Gln Val Tyr Gln Lys Leu Glu Lys Met Leu Ile Glu Lys Leu Asn Tyr 
            980                 985                 990         


Leu Val Phe Lys Asn Asn Asp Val  Asn Glu Thr Ala Gly  Val Leu Asn 
        995                 1000                 1005             


Ala Leu  Gln Leu Thr Asn Lys  Phe Glu Ser Phe Glu  Lys Met Gly 
    1010                 1015                 1020             


Lys Gln  Ser Gly Phe Leu Phe  Tyr Val Pro Ala Trp  Asn Thr Ser 
    1025                 1030                 1035             


Lys Ile  Asp Pro Ala Thr Gly  Phe Val Asp Phe Leu  Lys Pro Lys 
    1040                 1045                 1050             


Tyr Glu  Ser Val Glu Lys Ala  Lys Leu Phe Phe Glu  Lys Phe Glu 
    1055                 1060                 1065             


Ser Ile  Lys Phe Asn Ala Asp  Lys Asn Tyr Phe Glu  Phe Glu Phe 
    1070                 1075                 1080             


Asp Tyr  Lys Lys Phe Thr Glu  Lys Ala Glu Gly Ser  Gln Thr Lys 
    1085                 1090                 1095             


Trp Thr  Val Cys Thr His Ser  Asp Val Arg Tyr Arg  Tyr Asn Pro 
    1100                 1105                 1110             


Gln Thr  Lys Ala Ser Asp Glu  Val Asn Val Thr Asn  Glu Leu Lys 
    1115                 1120                 1125             


Leu Ile  Phe Asp Lys Phe Lys  Ile Glu Tyr Lys Asn  Gly Lys Asn 
    1130                 1135                 1140             


Leu Lys  Thr Glu Leu Leu Leu  Gln Asp Asp Lys Gln  Leu Phe Ser 
    1145                 1150                 1155             


Lys Leu  Leu His Tyr Leu Ala  Leu Thr Leu Met Leu  Arg Gln Ser 
    1160                 1165                 1170             


Lys Ser  Gly Thr Asp Ile Asp  Phe Ile Leu Ser Pro  Val Ala Lys 
    1175                 1180                 1185             


Asn Gly  Val Phe Tyr Asp Ser  Arg Asn Ala Met Pro  Asn Leu Pro 
    1190                 1195                 1200             


Lys Asp  Ala Asp Ala Asn Gly  Ala Phe His Ile Ala  Leu Lys Gly 
    1205                 1210                 1215             


Leu Trp  Cys Val Gln Gln Ile  Lys Lys Ala Asp Asp  Leu Lys Lys 
    1220                 1225                 1230             


Ile Lys  Leu Ala Ile Ser Asn  Lys Glu Trp Leu Ser  Phe Val Gln 
    1235                 1240                 1245             


Asn Leu  Lys 
    1250     


<210>  1358
<211>  1390
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1358

Met Ile Asp Asn Thr Lys Glu Lys Lys Glu Gly Ser Val Phe Asp Gly 
1               5                   10                  15      


Phe Thr Arg Lys Tyr Gln Leu Ser Lys Thr Leu Arg Phe Glu Leu Arg 
            20                  25                  30          


Pro Ile Leu Asn Thr Pro Lys Met Leu Asp Asp Glu Gln Val Ile Lys 
        35                  40                  45              


Asn Asp Glu Thr Arg Arg Lys Lys Tyr Glu Ala Val Lys Pro Trp Phe 
    50                  55                  60                  


Asp Gln Leu His Arg Glu Phe Ile Glu Asp Ala Leu Lys Ser Phe Lys 
65                  70                  75                  80  


Phe Lys Asn Leu Ala Ile Tyr Gln Asp Thr Phe Gln Thr Trp Gln Lys 
                85                  90                  95      


Asp Arg Lys Ser Lys Gln Lys Lys Asp Thr Leu Val Lys Ile Glu Val 
            100                 105                 110         


Gly Leu Arg Glu Glu Ile Val Arg Arg Phe Glu Glu Val Ala Asn Ile 
        115                 120                 125             


Trp Val Arg Ser Glu Gln Tyr Lys Leu Leu Gly Ile Lys Lys Glu Gly 
    130                 135                 140                 


Leu Gly Met Leu Phe Glu Ala Gly Val Phe Arg Leu Leu Lys Glu Arg 
145                 150                 155                 160 


Phe Lys Asn Glu Lys Asp Thr Thr Val Asp Gly Asn Asn Ile Phe Asp 
                165                 170                 175     


Glu Trp Thr Arg Trp Thr Gly Tyr Phe Lys Lys Phe Phe Glu Thr Arg 
            180                 185                 190         


Lys Asn Phe Tyr Lys Ser Asp Asp Thr Ser Thr Ala Ile Ala Tyr Arg 
        195                 200                 205             


Val Ile Asn Gln Asn Leu Arg Arg Phe Cys Glu Asn Ile Gln Ile Phe 
    210                 215                 220                 


Glu Lys Ile Ser Glu Lys Ile Glu Phe Ser Glu Val Glu Lys Ser Phe 
225                 230                 235                 240 


Asp Ile Ser Cys Ala Gly Ile Phe Ser Leu Ala Tyr Tyr Asn Ala Cys 
                245                 250                 255     


Leu Leu Gln Gly Gly Ile Asp Thr Tyr Asn Lys Ile Ile Gly Gly Glu 
            260                 265                 270         


Val Asp Glu Lys Asp Lys Lys Ile Pro Gly Ile Asn Glu Leu Ile Asn 
        275                 280                 285             


Lys Tyr Arg Gln Asp Asn Ser Gly Glu Lys Ile Pro Phe Leu Lys Gln 
    290                 295                 300                 


Leu Asp Lys Gln Ile His Ser Ala Lys Glu Ala Phe Ile Glu Ser Ile 
305                 310                 315                 320 


Glu Thr Asn Lys Glu Leu Val Gly Lys Leu Lys Thr Phe Tyr Glu Asn 
                325                 330                 335     


Ala Glu Val Lys Ile Gln Ser Phe Arg Asn Leu Ile Ala Asp Ile Val 
            340                 345                 350         


Thr Asp Tyr Ser Gly Tyr Asp Ile Asp Lys Ile Tyr Leu Thr Lys Glu 
        355                 360                 365             


Ala Val Ser His Asn Ala Ser Arg Trp Phe Ala Ser Phe Glu Ser Phe 
    370                 375                 380                 


Glu Arg Asp Leu Phe Ala Val Val Ala Glu Lys Gln Asn Lys Leu Val 
385                 390                 395                 400 


Tyr Glu Leu Leu Arg Thr His Lys Asn Asp Ser Lys Ile Ser Asp Lys 
                405                 410                 415     


Asp Gly Lys Phe Ser Phe Pro Asp Phe Ile Lys Cys Ser His Ile Lys 
            420                 425                 430         


Arg Ala Leu Glu Lys Gln Glu Gly Arg Ile Trp Lys Gly Glu Tyr Tyr 
        435                 440                 445             


Glu Asp Ile Val Asp Phe Glu Lys Ile Lys Asp Val Phe Thr Gln Phe 
    450                 455                 460                 


Leu Cys Val Phe Lys Phe Glu Leu Glu Gln Gln Phe Phe Arg Lys Thr 
465                 470                 475                 480 


Thr Ser Ala Gln Thr Gly Glu Gln Thr Lys Ile Gly Tyr Glu Ile Phe 
                485                 490                 495     


Val Thr Lys Ile Asn Glu Leu Ile Thr Arg Glu Asn Pro Val Ile Asp 
            500                 505                 510         


Leu Glu Glu Lys Ile Ala Ile Lys Asn Phe Ala Asp Ala Thr Leu Leu 
        515                 520                 525             


Ile Tyr Gln Ile Ala Lys Tyr Phe Ala Val Glu Lys Arg Arg Gly Trp 
    530                 535                 540                 


Leu Asp Asn Tyr Asp Leu Asp Asp Arg Phe Tyr Lys Ser Ser Asp Ile 
545                 550                 555                 560 


Gly Tyr Leu Asn Phe Tyr Arg Asp Ala Phe Glu Gln Ile Val Arg Pro 
                565                 570                 575     


Tyr Asn Leu Phe Arg Asn Tyr Leu Thr Lys Lys Pro Tyr Asn Thr Asn 
            580                 585                 590         


Lys Trp Val Leu Ser Phe Glu Asn Pro Thr Leu Ala Asp Gly Trp Asp 
        595                 600                 605             


Lys Asn Lys Glu Lys Thr Asn Ala Ala Val Ile Leu Arg Lys Asp Gly 
    610                 615                 620                 


Arg Tyr Tyr Leu Gly Ile Ile Lys Glu Asp Cys Lys Ser Leu Phe Ala 
625                 630                 635                 640 


Asp Arg Tyr Ser Lys Glu Met Ser Glu Gly Ile Glu Ser Gly Ser Phe 
                645                 650                 655     


Gln Lys Met Ala Tyr Lys Phe Phe Pro Glu Ala Ser Lys Met Ile Pro 
            660                 665                 670         


Lys Cys Ser Thr Gln Thr Lys Asn Val Lys Glu His Phe Arg Lys Ser 
        675                 680                 685             


Ser Ser Asp Tyr Asn Leu Phe His Glu Lys Asp Tyr Lys Ile Ser Val 
    690                 695                 700                 


Ala Ile Thr Lys Asn Ile Tyr Glu Leu Asn Asn Val Phe Tyr Arg Lys 
705                 710                 715                 720 


Asp Asn Ile Glu Glu Ser Phe Val Pro Lys Asn Asp Phe Glu Lys Lys 
                725                 730                 735     


Leu Gly Val Lys Lys Phe Gln Arg Gln Tyr Leu Glu Ile Ser Arg Asp 
            740                 745                 750         


Asn Asn Gly Tyr Lys Gln Ala Leu Ala Gln Trp Ile Glu Phe Cys Ile 
        755                 760                 765             


Arg Phe Leu Lys Ala Tyr Lys Ser Thr Thr Ile Phe Asp Tyr Ser Arg 
    770                 775                 780                 


Leu Arg Glu Ala Lys Glu Tyr Glu Ser Leu Asp Ala Phe Tyr Gln Asp 
785                 790                 795                 800 


Ile Asn Ala Leu Thr Tyr Asn Ile Ser Phe Val Pro Ile Ser Glu Gln 
                805                 810                 815     


Tyr Ile Lys Glu Lys Asn Asp Asn Gly Glu Leu Phe Leu Phe Glu Ile 
            820                 825                 830         


Tyr Asn Lys Asp Trp Ser Leu Gly Pro Met Asp Lys Asn Arg Lys Arg 
        835                 840                 845             


Thr Lys Asn Leu His Thr Leu Tyr Phe Glu Gln Leu Phe Ser Lys Glu 
    850                 855                 860                 


Asn Glu Gln Glu Asn Phe Leu Phe Gln Leu Asn Gly Glu Ala Glu Leu 
865                 870                 875                 880 


Phe Phe Arg Pro Lys Thr Glu Glu Lys Arg Leu Gly Tyr Lys Val Trp 
                885                 890                 895     


Asp Ala Gly Glu Lys Lys Trp Val Lys Ala Lys Glu Lys Glu Asp Gly 
            900                 905                 910         


Ala Val Ile Asp Arg Lys Arg Tyr Ala Lys Asp Ile Ile Leu Phe His 
        915                 920                 925             


Cys Pro Ile Thr Leu Asn Arg Val Ser Glu Ser Lys Thr Lys Arg Glu 
    930                 935                 940                 


Met Asp Val Glu Ile Arg Glu Val Leu Ser Ser Thr Pro Gly Val His 
945                 950                 955                 960 


Ile Ile Gly Val Asp Arg Gly Glu Lys His Leu Ala Tyr Tyr Ser Val 
                965                 970                 975     


Ile Asp Gln Asn Gly Lys Ile Ile Glu Thr Asp Thr Leu Asn Ser Ile 
            980                 985                 990         


Gly Lys Asp Gly Arg Gly Lys Pro  Val Glu Tyr Ala Ser  Lys Leu Glu 
        995                 1000                 1005             


Lys Arg  Ala Gln Glu Arg Glu  Ala Ser Arg Arg Asp  Trp Glu Glu 
    1010                 1015                 1020             


Val Glu  Ala Ile Lys Asp Leu  Lys Lys Gly Tyr Ile  Ser Gln Val 
    1025                 1030                 1035             


Ile Arg  Asn Leu Ala Asp Leu  Ile Ile Lys His Asn  Ala Ile Ile 
    1040                 1045                 1050             


Val Phe  Glu Asp Leu Asn Met  Arg Phe Lys Gln Ile  Arg Gly Gly 
    1055                 1060                 1065             


Ile Glu  Lys Ser Ala Tyr Gln  Gln Leu Glu Arg Ala  Leu Ile Asp 
    1070                 1075                 1080             


Lys Leu  Ser Phe Leu Val Lys  Lys Gly Glu Glu Asp  Pro Lys Gln 
    1085                 1090                 1095             


Thr Gly  His Ile Leu Arg Ala  Tyr Gln Leu Ala Ala  Pro Val Ile 
    1100                 1105                 1110             


Ala Phe  Lys Asp Met Gly Lys  Gln Thr Gly Leu Ile  Phe Tyr Thr 
    1115                 1120                 1125             


Gln Ala  Gly Tyr Thr Ser Lys  Thr Cys Pro Glu Cys  Gly Tyr Arg 
    1130                 1135                 1140             


Arg Asn  Ile Lys Cys Leu Phe  Glu Asn Ile Glu Gln  Ala Lys Thr 
    1145                 1150                 1155             


Leu Ile  Glu Asn Leu Glu Ser  Ile Asn Tyr Asn Lys  Lys Glu Asp 
    1160                 1165                 1170             


Val Phe  Gln Ile Ser Tyr Ser  Leu Glu Lys Leu Ser  Ser Lys Asp 
    1175                 1180                 1185             


Gln Lys  Lys Glu Lys Lys Val  Ser Asn Glu Leu Tyr  Ala Lys Thr 
    1190                 1195                 1200             


Leu Lys  Lys Asp Ile Phe Ile  Leu Thr Thr Lys Asn  Ala Leu Arg 
    1205                 1210                 1215             


Tyr Lys  Trp Tyr Asp Arg Tyr  Ser Glu Lys Ala Lys  Val Ala Lys 
    1220                 1225                 1230             


Arg Gly  Ile Asp Glu Tyr Lys  Gly Glu Val Asn Glu  Ser Glu Thr 
    1235                 1240                 1245             


Lys Lys  Gly Val Val Lys Glu  Phe Asn Leu Thr Glu  Tyr Leu Lys 
    1250                 1255                 1260             


Gly Leu  Leu Lys Thr Tyr Glu  Ile Asp Tyr Glu His  Gly Gly Ile 
    1265                 1270                 1275             


Arg Glu  Gln Ile Leu Ser Val  Ala Arg Gly Arg Glu  Phe Tyr Lys 
    1280                 1285                 1290             


Asp Phe  Leu Tyr Ala Leu Phe  Leu Leu Thr Glu Thr  Arg His Ser 
    1295                 1300                 1305             


Ile Ser  Gly Arg Asn Thr Asp  Tyr Ile Gln Cys Pro  Glu Cys Glu 
    1310                 1315                 1320             


Phe Asp  Ser Arg Lys Gly Phe  Lys Asp Ile Lys Glu  Phe Asn Gly 
    1325                 1330                 1335             


Asp Ala  Asn Gly Ala Tyr Asn  Ile Ala Arg Lys Gly  Ile Met Ile 
    1340                 1345                 1350             


Leu Glu  Lys Ile Lys Gln Phe  Lys Lys Asp Asn Asp  Gly Asn Leu 
    1355                 1360                 1365             


Glu Lys  Met Gly Trp Gly Asp  Leu Ser Ile Ser Ile  Glu Glu Trp 
    1370                 1375                 1380             


Asp Lys  Phe Thr Gln Lys Glu  
    1385                 1390 


<210>  1359
<211>  1341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1359

Met Asn Ala Glu Lys Lys Cys Ala Phe Asn Glu Phe Thr Asn Leu Tyr 
1               5                   10                  15      


Glu Leu Ser Lys Thr Leu Lys Phe Glu Leu Lys Pro Val Gly Lys Thr 
            20                  25                  30          


Lys Glu Ile Ile Lys Asn Phe Arg Glu Glu Leu Glu Lys Asn Asn Pro 
        35                  40                  45              


Ile Gln Leu Asp Lys Glu Ile Ser Ile Ala Lys Gln Glu Ile Lys Lys 
    50                  55                  60                  


Asp Leu Asp Ile Leu His Arg Glu Phe Val Arg Gln Ser Phe Glu Leu 
65                  70                  75                  80  


Ala Phe Lys Glu Asn Lys Ile Asn Tyr Glu Ile Phe Phe Glu Ile Tyr 
                85                  90                  95      


Arg Asn Phe Lys Lys Phe Ser Lys Asn Lys Lys Ser Asp Glu Tyr Ile 
            100                 105                 110         


Ala Ala Ile Lys Asn Leu Lys Glu Glu Lys Ile Lys Leu Val Lys Lys 
        115                 120                 125             


Ile Ala Asp Tyr Phe Val Lys Met Asp Gln Glu Trp Arg Lys Tyr Tyr 
    130                 135                 140                 


Pro Ser Glu Lys Lys Ser Phe Leu Leu Met Ser Asn Gly Val Ile Asn 
145                 150                 155                 160 


Leu Leu Ile Asp Ile Asn Lys Asp Arg Met Asp Tyr Ser Phe Ile Pro 
                165                 170                 175     


Lys Glu Val Cys Glu Lys Ile Lys Gly Lys Gly Lys Ile Glu Leu Tyr 
            180                 185                 190         


Glu Lys Phe Asn Lys His Phe Gly Tyr Leu Ser Lys Tyr Asn Thr Ala 
        195                 200                 205             


Arg Glu Asn Phe Tyr Ser Asp Asp Val Lys Asn Ser Ala Ser Val Ala 
    210                 215                 220                 


Tyr Arg Ala Ile Cys Glu Asn Leu Glu Lys Phe Cys Asp Asn Lys Ile 
225                 230                 235                 240 


Lys Val Glu Ala Asp Asn Phe Pro Lys Glu Ile Lys Glu Lys Phe Leu 
                245                 250                 255     


Glu Tyr Phe Asn Lys Ile Thr Asp Leu Ser Phe Tyr Asn Arg Cys Leu 
            260                 265                 270         


Ser Gln Asn Gly Ile Glu Glu Tyr His Lys Phe Lys Asp Glu Ile Asn 
        275                 280                 285             


Arg Ser Ile Asn Glu Leu Tyr Pro Asn Asn Ser Lys Asn Lys Pro Gln 
    290                 295                 300                 


Asn Ile Pro Tyr Leu Ser Lys Leu Asp Lys Gln Ile Leu Ser Glu Lys 
305                 310                 315                 320 


Asp Lys Gln Ser Trp Ile Glu Glu Gly Phe Ile Asp Glu Asn Asn Phe 
                325                 330                 335     


Ile Phe Glu Phe Glu Lys Phe Ile Lys Met Asn Asp Lys Tyr Ile Pro 
            340                 345                 350         


Ser Glu Lys Lys Leu Phe Asn Glu Phe Phe Ile Phe Gln Asn Asn Asp 
        355                 360                 365             


Glu Val Leu Gln Lys Leu Phe Leu Leu Lys Lys Thr Ile Asn Asn Phe 
    370                 375                 380                 


Ala Asn Ile Tyr Leu Asp Asp Pro Lys Asn Leu Met Asp Leu Met Pro 
385                 390                 395                 400 


Ser Lys Gly Thr Lys Lys Arg Asp Tyr Leu Ser Leu Leu Glu Ile Lys 
                405                 410                 415     


Glu Ala Leu Asn Ser Tyr Ala Glu Asn Gln Asn Ile Gly Glu Thr Gln 
            420                 425                 430         


Ile Phe Lys Ile Lys Tyr Asp Asp Val Ile Asp Lys Thr Lys Thr Pro 
        435                 440                 445             


Phe Glu Cys Phe Met Lys Ile Trp Lys Tyr Glu Tyr Asp Leu Leu Tyr 
    450                 455                 460                 


Asn Glu Gly Asp Gly Lys Lys Asn Ile Glu Glu Gly Trp Glu Lys Ile 
465                 470                 475                 480 


Cys Glu Lys Leu Gln Asn Lys Lys Asp Glu Phe Thr Gly Asp Tyr Gly 
                485                 490                 495     


Lys Tyr Lys Lys Glu Ile Lys Asp Tyr Cys Glu Met Ala Leu Ser Leu 
            500                 505                 510         


Ser Gln Arg Ile Lys His Leu Asp Ile Arg Ile Lys Lys Arg Asp Gly 
        515                 520                 525             


Ser Glu Asp Ala Asp Asn Asn Leu Phe Glu Gly Val Glu Lys Leu Asp 
    530                 535                 540                 


Glu Phe Tyr Asp Arg Leu Phe Glu Ile Val Asp Glu His Asn Ala Tyr 
545                 550                 555                 560 


Gln Tyr His His Glu Phe Gln Ala Phe Leu Thr Lys Lys Pro Tyr Lys 
                565                 570                 575     


Thr Asp Lys Phe Lys Leu Ser Phe Asp Asn Pro Gln Leu Leu Lys Gly 
            580                 585                 590         


Trp Asp Leu Asn Lys Glu Pro Asp Tyr Phe Gly Ala Leu Leu Val Lys 
        595                 600                 605             


Asn Glu Lys Tyr Phe Leu Ala Val Phe His Lys Gln Tyr Ala Asn Glu 
    610                 615                 620                 


Ile Phe Arg Gln Lys Asn Lys Asn Ser Thr Val Lys Ile Lys Glu Ala 
625                 630                 635                 640 


Tyr Asp Val Phe Asn Gly Asn Tyr Tyr Lys Lys Val Glu Leu Lys Gln 
                645                 650                 655     


Ile Pro Lys Leu Phe Met Asn Leu Pro Arg Ile Cys Phe Ala Lys Ser 
            660                 665                 670         


Asn Gln Glu Glu Phe Gly Leu Thr Ile Glu Leu Glu Lys Ile Lys Ser 
        675                 680                 685             


Glu Phe Asp Lys Phe Gln Lys Leu Lys Lys Glu Arg Gln Asp Asn Asn 
    690                 695                 700                 


Leu Lys Phe Asp Gln Glu Lys Phe Ile Lys Leu Leu Ala Tyr Tyr Arg 
705                 710                 715                 720 


His Cys Leu Glu Ile Arg Glu Asp Trp Lys Val Phe Lys Tyr Asn Phe 
                725                 730                 735     


Lys Pro Leu Tyr Lys Tyr Ser Asn Leu Gly Glu Phe Tyr Arg Asp Leu 
            740                 745                 750         


Glu Lys Asp Thr Tyr Asn Leu Asp Leu Val Arg Val Asn Ser Glu Tyr 
        755                 760                 765             


Val Glu Lys Lys Arg Asn Glu Gly Lys Leu Tyr Leu Phe Glu Val Tyr 
    770                 775                 780                 


Asn Lys Asp Phe Ser Asp Lys Ser Ile Gly Arg Lys Asn Ile His Ser 
785                 790                 795                 800 


Asp Tyr Phe Lys Ser Leu Phe Leu Pro Asp Asn Leu Thr Asn Lys Lys 
                805                 810                 815     


Ile Ala Leu Ala Ala Asp Ala Glu Ile Phe Phe Arg Glu Lys Ser Leu 
            820                 825                 830         


Lys Asn Lys Asp Glu Ile Ile Thr Gln Lys Asn Lys Ile Lys Ile Asp 
        835                 840                 845             


Lys Asn Asn His Lys Gly Glu Lys Ala Tyr Ser Lys Asn Arg Tyr Thr 
    850                 855                 860                 


Asp Asp Lys Ile Ile Leu His Leu Pro Ile Asn Leu Asn Phe Gly Glu 
865                 870                 875                 880 


Asn Lys Asn His Lys Asp Phe Asn Tyr Glu Ile Asn Asp Ser Leu Leu 
                885                 890                 895     


Lys Asn Asn Arg Asp Ile Lys Ile Ile Gly Ile Asp Arg Gly Glu Lys 
            900                 905                 910         


Tyr Leu Leu Tyr Tyr Ser Val Ile Asp Gln Lys Gly Asp Ile Lys Glu 
        915                 920                 925             


Met Gly Asp Phe Asn Val Val Asn Gly Val Asp Tyr Tyr Ser Lys Leu 
    930                 935                 940                 


Val Ala Tyr Glu Glu Lys Arg Lys Gln Ala Arg Leu Asp Trp Gly Asp 
945                 950                 955                 960 


Ile Gln Ser Ile Lys Glu Leu Lys Glu Gly Tyr Ile Ser His Val Ile 
                965                 970                 975     


His Glu Ile Cys Asp Leu Ile Lys Lys Tyr Asn Ala Ile Val Val Leu 
            980                 985                 990         


Glu Asp Leu Ser Met Gly Phe Lys  Arg Gly Arg Phe Phe  Arg Glu Arg 
        995                 1000                 1005             


Gln Val  Tyr Gln Lys Phe Glu  Leu Ala Leu Phe Arg  Lys Leu Asn 
    1010                 1015                 1020             


Tyr Trp  Val Asp Lys Asn Lys  Glu Glu Gly Glu Lys  Gly Ser Ala 
    1025                 1030                 1035             


Leu Ser  Ala Trp Gln Leu Thr  Pro Asn Glu Lys Asp  Phe Lys Val 
    1040                 1045                 1050             


Ser Gln  Leu Glu Lys Ala Arg  Gln Leu Gly Ile Leu  Phe Tyr Thr 
    1055                 1060                 1065             


Ala Ala  Gly Tyr Thr Ser Lys  Thr Cys Pro Asp Cys  Gly Phe Arg 
    1070                 1075                 1080             


Lys Asn  Ile Thr Leu Lys Tyr  Val Asn Asp Lys Gln  Leu Lys Glu 
    1085                 1090                 1095             


Asp Leu  Gly Lys Leu Lys Ile  Asn Phe Val Glu Asp  His Phe Lys 
    1100                 1105                 1110             


Ile Lys  Tyr Asn Leu Leu Asp  Phe Glu Lys Asn Lys  Lys Ser Glu 
    1115                 1120                 1125             


Lys Ala  Lys Asp Thr Val Leu  Pro Lys Glu Glu Arg  Lys Ile Ser 
    1130                 1135                 1140             


Phe Val  Ala Asn Ser Lys Val  Ile Arg Ile Arg Trp  His Asp Ile 
    1145                 1150                 1155             


Lys Thr  Lys Leu Ala Lys Arg  Glu Leu Asp Gly Glu  Lys Ile Ile 
    1160                 1165                 1170             


Lys Lys  Phe Pro Lys Gly Val  Ile Lys Glu Tyr Asp  Ile Asn Ile 
    1175                 1180                 1185             


Cys Leu  Lys Arg Ile Ile Gly  Asp Lys Ile Asp Phe  Ser Lys Asp 
    1190                 1195                 1200             


Ser Ile  Thr Glu Gln Leu Ile  Asn Gly Arg Phe Gly  Lys Asp Phe 
    1205                 1210                 1215             


Tyr Trp  Glu Leu Met Tyr Phe  Leu Asn Leu Ile Leu  Glu Ile Arg 
    1220                 1225                 1230             


Asn Val  Val Ser Glu Thr Glu  Ile Asp Tyr Ile Gln  Cys Pro Val 
    1235                 1240                 1245             


Cys Gly  Phe His Ser Asp Asp  Gly Phe Arg Gly Asn  Lys Phe Asn 
    1250                 1255                 1260             


Gly Asp  Ala Asn Gly Ala Tyr  Asn Ile Ala Arg Lys  Gly Arg Leu 
    1265                 1270                 1275             


Ile Leu  Glu Lys Ile Asn Gln  His Lys Asn Leu Lys  Glu Leu Lys 
    1280                 1285                 1290             


Thr Gly  Asp Leu Ser Val Asn  Ile Val Glu Trp Asp  Lys Ala Val 
    1295                 1300                 1305             


Asn Asn  Trp Asp Lys Phe Val  Gln Gly Asp Asn Thr  Glu Ser Lys 
    1310                 1315                 1320             


Asn Ile  Glu Ile Pro Ala Lys  Glu Glu Met Ile Ser  Val Glu Val 
    1325                 1330                 1335             


Arg Glu  Ser 
    1340     


<210>  1360
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1360

Met Lys Lys Glu Lys Glu Phe Lys Ser Phe Gly Asp Phe Thr Asn Leu 
1               5                   10                  15      


Tyr Glu Ile Ser Lys Thr Leu Arg Phe Glu Leu Lys Pro Val Glu Asn 
            20                  25                  30          


Thr Gln Thr Met Leu Asp Glu Ala Asp Val Phe Gly Lys Asp Lys Val 
        35                  40                  45              


Ile Lys Asp Lys Tyr Thr Lys Thr Lys Pro Phe Ile Asp Lys Leu His 
    50                  55                  60                  


Arg Glu Phe Val Asp Glu Ser Leu Lys Asp Val Ser Leu Ser Gly Leu 
65                  70                  75                  80  


Lys Lys Tyr Ser Glu Val Leu Glu Asn Trp Lys Lys Asn Lys Lys Asp 
                85                  90                  95      


Lys Asp Ile Val Lys Glu Leu Lys Lys Glu Glu Glu Arg Leu Arg Lys 
            100                 105                 110         


Glu Val Val Glu Phe Phe Asp Asn Thr Ala Lys Lys Trp Ala Asn Glu 
        115                 120                 125             


Lys Tyr Lys Glu Leu Gly Leu Lys Lys Lys Asp Ile Gly Ile Leu Phe 
    130                 135                 140                 


Glu Glu Ser Val Phe Asp Leu Leu Lys Glu Lys Tyr Gly Glu Glu Gln 
145                 150                 155                 160 


Asp Ser Phe Leu Lys Glu Glu Lys Gly Asp Phe Leu Lys Asn Glu Lys 
                165                 170                 175     


Gly Glu Lys Val Ser Ile Phe Asp Glu Trp Lys Gly Phe Val Gly Tyr 
            180                 185                 190         


Phe Thr Lys Phe Gln Glu Thr Arg Lys Asn Phe Tyr Lys Asn Asp Gly 
        195                 200                 205             


Thr Glu Thr Ala Leu Ala Thr Arg Ile Ile Asp Gln Asn Leu Lys Arg 
    210                 215                 220                 


Phe Cys Asp Asn Ile Asp Asp Phe Lys Lys Ile Lys Asn Lys Ile Asp 
225                 230                 235                 240 


Phe Ser Glu Val Glu Lys Asn Phe Asn Lys Thr Ala Asp Val Phe Ser 
                245                 250                 255     


Leu Asp Phe Tyr Asn Gln Cys Leu Leu Gln Lys Gly Ile Asp Ser Tyr 
            260                 265                 270         


Asn Glu Phe Ile Gly Gly Lys Thr Leu Glu Asn Gly Lys Lys Leu Lys 
        275                 280                 285             


Gly Val Asn Glu Leu Val Asn Glu Tyr Arg Gln Lys Asn Lys Asn Glu 
    290                 295                 300                 


Lys Val Ser Phe Leu Lys Leu Leu Asp Lys Gln Ile Leu Ser Glu Lys 
305                 310                 315                 320 


Glu Lys Leu Ser Phe Gly Ile Glu Asn Asp Glu Gln Leu Leu Val Val 
                325                 330                 335     


Leu Asn Ser Phe Tyr Glu Thr Ala Glu Glu Lys Thr Lys Ile Leu Arg 
            340                 345                 350         


Thr Leu Phe Gly Asp Phe Val Glu His Asn Glu Asn Tyr Asp Leu Asp 
        355                 360                 365             


Lys Thr Tyr Ile Ser Lys Val Ala Phe Asn Thr Ile Ser His Lys Trp 
    370                 375                 380                 


Thr Asn Glu Thr His Lys Phe Glu Glu Leu Leu Tyr Gly Ala Met Lys 
385                 390                 395                 400 


Glu Asp Lys Pro Ile Gly Leu Asn Tyr Asp Lys Lys Glu Asp Ser Tyr 
                405                 410                 415     


Lys Phe Pro Asp Phe Ile Ala Leu Gly Tyr Leu Lys Lys Cys Leu Asn 
            420                 425                 430         


Asn Leu Asp Cys Asp Thr Lys Phe Trp Lys Glu Lys Tyr Tyr Glu Asn 
        435                 440                 445             


Asn Ala Asp Lys Lys Asp Lys Asp Lys Gly Phe Leu Thr Gly Gly Gln 
    450                 455                 460                 


Asn Ala Trp Asp Gln Phe Leu Gln Ile Phe Ile Phe Glu Phe Asn Gln 
465                 470                 475                 480 


Leu Phe Asn Ser Glu Ala Phe Asp Asn Lys Gly Lys Glu Ile Lys Ile 
                485                 490                 495     


Gly Tyr Asp Asn Phe Arg Lys Asp Phe Glu Glu Ile Ile Asn Gln Lys 
            500                 505                 510         


Asp Phe Lys Asn Asp Glu Asn Leu Lys Ile Ala Ile Lys Asn Phe Ala 
        515                 520                 525             


Asp Ser Val Leu Trp Ile Tyr Gln Met Ala Lys Tyr Phe Ala Ile Glu 
    530                 535                 540                 


Lys Lys Arg Gly Trp Asp Asp Asp Phe Glu Leu Ser Glu Phe Tyr Thr 
545                 550                 555                 560 


Asn Pro Ser Asn Gly Tyr Ser Leu Phe Tyr Asp Arg Ala Tyr Glu Glu 
                565                 570                 575     


Ile Val Gln Lys Tyr Asn Asp Leu Arg Asn Tyr Leu Thr Lys Lys Pro 
            580                 585                 590         


Tyr Lys Glu Asp Lys Trp Lys Leu Asn Phe Glu Asn Pro Thr Leu Ala 
        595                 600                 605             


Asn Gly Phe Asp Lys Asn Lys Glu Ser Asp Asn Ser Thr Val Ile Leu 
    610                 615                 620                 


Arg Lys Lys Arg Lys Tyr Tyr Leu Gly Leu Met Lys Lys Gly Asn Asn 
625                 630                 635                 640 


Lys Ile Phe Glu Asp Arg Asn Lys Ala Glu Phe Ile Arg Asn Ile Glu 
                645                 650                 655     


Ser Gly Ala Tyr Glu Lys Met Ala Tyr Lys Tyr Leu Pro Asp Val Ala 
            660                 665                 670         


Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Asn Glu Ala Lys Asn His 
        675                 680                 685             


Phe Arg Asn Ser Ala Asp Asp Leu Glu Ile Lys Lys Ser Phe Ser Asn 
    690                 695                 700                 


Pro Leu Lys Ile Thr Lys Arg Ile Phe Asp Leu Asn Asn Ile Gln Tyr 
705                 710                 715                 720 


Asp Lys Thr Asn Val Ser Lys Lys Ile Ser Gly Asp Asn Lys Gly Ile 
                725                 730                 735     


Lys Ile Phe Gln Lys Glu Tyr Tyr Lys Ile Ser Gly Asp Phe Asp Val 
            740                 745                 750         


Tyr Lys Ser Ala Leu Asn Asp Trp Ile Asp Phe Cys Lys Asp Phe Leu 
        755                 760                 765             


Ser Lys Tyr Asp Ser Thr Lys Asp Phe Asp Phe Ser Ile Leu Arg Lys 
    770                 775                 780                 


Thr Lys Asp Tyr Lys Ser Leu Asp Glu Phe Tyr Val Asp Val Ala Lys 
785                 790                 795                 800 


Ile Thr Tyr Lys Ile Ser Phe Thr Pro Val Ser Glu Ser Tyr Ile Asp 
                805                 810                 815     


Gln Lys Asn Lys Asn Gly Glu Leu Tyr Leu Phe Glu Ile Tyr Asn Gln 
            820                 825                 830         


Asp Phe Ala Lys Gly Lys Met Gly Ala Lys Asn Leu His Thr Leu Tyr 
        835                 840                 845             


Phe Glu Asn Val Phe Ser Pro Glu Asn Ile Ser Lys Asn Phe Pro Ile 
    850                 855                 860                 


Lys Leu Asn Gly Asn Ala Glu Leu Phe Phe Arg Pro Lys Ser Ile Glu 
865                 870                 875                 880 


Ser Lys Lys Glu Lys Arg Asn Phe Val Arg Glu Ile Val Asn Lys Lys 
                885                 890                 895     


Arg Tyr Ser Glu Asp Lys Ile Phe Phe His Cys Pro Ile Thr Leu Asn 
            900                 905                 910         


Arg Glu Thr Gly Ser Ile Tyr Arg Phe Asn Asn Tyr Val Asn Asn Phe 
        915                 920                 925             


Leu Ser Glu Asn Asn Ile Asn Ile Ile Gly Val Asp Arg Gly Glu Lys 
    930                 935                 940                 


His Leu Ala Tyr Tyr Ser Val Ile Asp Lys Asn Gly Val Lys Ile Gly 
945                 950                 955                 960 


Gly Gly Ser Phe Asn Glu Ile Asn Lys Val Asp Tyr Ala Lys Lys Leu 
                965                 970                 975     


Glu Glu Arg Ala Gly Glu Arg Glu Gln Ser Arg Lys Asp Trp Gln Val 
            980                 985                 990         


Val Glu Gly Ile Lys Asp Leu Lys  Lys Gly Tyr Ile Ser  Gln Val Val 
        995                 1000                 1005             


Arg Glu  Leu Ala Asp Leu Ala  Ile Lys His Asn Ala  Ile Ile Val 
    1010                 1015                 1020             


Leu Glu  Asp Leu Asn Met Arg  Phe Lys Gln Ile Arg  Gly Gly Ile 
    1025                 1030                 1035             


Glu Lys  Ser Ile Tyr Gln Gln  Leu Glu Lys Ala Leu  Ile Asp Lys 
    1040                 1045                 1050             


Leu Ser  Phe Leu Val Glu Lys  Gly Glu Lys Asp Pro  Asn Gln Ala 
    1055                 1060                 1065             


Gly His  Ile Leu Lys Ala Tyr  Gln Leu Ala Ala Pro  Phe Thr Ser 
    1070                 1075                 1080             


Phe Lys  Asp Met Gly Lys Gln  Thr Gly Ile Val Phe  Tyr Thr Gln 
    1085                 1090                 1095             


Ala Ser  Tyr Thr Ser Lys Thr  Cys Pro Asn Cys Gly  Phe Arg Lys 
    1100                 1105                 1110             


Asn Asn  Asn Lys Phe Tyr Phe  Glu Asn Asn Ile Gly  Lys Ala Gln 
    1115                 1120                 1125             


Asp Ala  Leu Lys Lys Leu Lys  Thr Phe Glu Tyr Asp  Ser Glu Asn 
    1130                 1135                 1140             


Lys Cys  Phe Gly Leu Ser Tyr  Cys Leu Ser Asp Phe  Ala Asn Lys 
    1145                 1150                 1155             


Glu Glu  Val Glu Lys Asn Lys  Asn Lys Lys Arg Asn  Asn Ala Pro 
    1160                 1165                 1170             


Tyr Ser  Asp Ile Glu Lys Lys  Asp Cys Phe Glu Leu  Ser Thr Lys 
    1175                 1180                 1185             


Asp Ala  Val Arg Tyr Arg Trp  His Asp Lys Asn Thr  Glu Arg Gly 
    1190                 1195                 1200             


Lys Thr  Phe Phe Glu Gly Glu  Ser Val Tyr Glu Glu  Lys Glu Glu 
    1205                 1210                 1215             


Lys Glu  Ile Gly Gln Thr Lys  Arg Gly Leu Val Lys  Glu Tyr Asp 
    1220                 1225                 1230             


Ile Ser  Lys Cys Leu Ile Gly  Leu Phe Glu Lys Thr  Gly Leu Asp 
    1235                 1240                 1245             


Tyr Lys  Gln Asn Leu Leu Asp  Lys Ile Asn Ser Gly  Lys Phe Asp 
    1250                 1255                 1260             


Gly Thr  Phe Tyr Lys Asn Leu  Phe Asn Tyr Leu Asn  Leu Leu Phe 
    1265                 1270                 1275             


Glu Ile  Arg Asn Ser Ile Ser  Gly Thr Glu Ile Asp  Tyr Ile Ser 
    1280                 1285                 1290             


Cys Pro  Glu Cys Gln Phe His  Thr Asp Lys Ser Lys  Thr Ile Lys 
    1295                 1300                 1305             


Asn Gly  Asp Asp Asn Gly Ser  Tyr Asn Ile Ala Arg  Lys Gly Met 
    1310                 1315                 1320             


Ile Ile  Leu Asp Lys Ile Lys  Gln Phe Lys Lys Glu  Asn Gly Ser 
    1325                 1330                 1335             


Leu Asp  Lys Met Gly Trp Gly  Glu Leu Phe Ile Asp  Leu Glu Glu 
    1340                 1345                 1350             


Trp Asp  Lys Phe Ala Gln Lys  Lys Asn Asn Asn Ile  Ile Asp Lys 
    1355                 1360                 1365             


<210>  1361
<211>  1382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1

<400>  1361

Met Ser Lys Asn Asn Val Trp Asp Lys Phe Thr Asn Gln Tyr Ser Leu 
1               5                   10                  15      


Ser Lys Thr Leu Arg Phe Glu Leu Lys Pro Val Gly Lys Thr Arg Glu 
            20                  25                  30          


Lys Met Asn Glu Gln Leu Lys Tyr Asp Lys Gln Leu Gln Thr Phe Leu 
        35                  40                  45              


Lys Asp Gln Glu Ile Glu Asp Ala Tyr Gln Tyr Leu Lys Pro Phe Phe 
    50                  55                  60                  


Asp Lys Leu His Lys Glu Phe Ile Thr Asp Ser Leu Asp Ser Asp Thr 
65                  70                  75                  80  


Thr Lys Lys Ile Asp Phe Ser Asp Tyr Leu Ile Asp Tyr Arg Asp Lys 
                85                  90                  95      


Lys Asp Leu Thr Lys Lys Glu Asp Ser Leu Arg Lys Asn Ile Gly Glu 
            100                 105                 110         


Val Phe Val Lys Ala Gly Asp Arg Trp Lys Lys Glu Lys Tyr Pro Lys 
        115                 120                 125             


Tyr Glu Trp Lys Lys Gly Ser Asn Val Ala Asn Gly Ser Gly Ile Leu 
    130                 135                 140                 


Leu Thr Gln Asp Val Leu Met Leu Ile Lys Asp Leu Asn Pro Asn Asp 
145                 150                 155                 160 


Gln Lys Ile Lys Lys Met Ile Glu Glu Thr Phe Lys Gly Phe Phe Thr 
                165                 170                 175     


Tyr Phe Ser Gly Phe Asn Leu Asn Arg Glu Asn Tyr Tyr Thr Thr Lys 
            180                 185                 190         


Val Glu Lys Ala Thr Ala Val Ala Thr Arg Ile Val His Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Cys Asp Asn Leu Val Gln Phe Glu Cys Ile Ile Lys Lys 
    210                 215                 220                 


Lys Lys Asn Gly Glu Val Asp Arg Ile Glu Arg Lys Ser Glu Tyr Leu 
225                 230                 235                 240 


Asn Ala Tyr Gln Tyr Leu Lys Ser Lys Gly Arg Thr Thr Gln Ile Lys 
                245                 250                 255     


Asp Ala Glu Ser Gly Lys Met Ile Glu Ala Glu Val Ile Asn Glu Asn 
            260                 265                 270         


Met Phe Ser Ile Ser Tyr Phe Ser Ala Cys Leu Ser Gln Ser Gly Ile 
        275                 280                 285             


Glu Glu Tyr Asn Arg Thr Ile Gly His Tyr Asn Leu Leu Ile Asn Leu 
    290                 295                 300                 


Tyr Asn Gln Ala Lys Glu Gly Glu Glu Lys Asp Leu Lys Lys Ser Glu 
305                 310                 315                 320 


Lys Lys Phe Lys Arg Leu Pro Ile Phe Lys Thr Leu Tyr Lys Gln Ile 
                325                 330                 335     


Gly Cys Gly Glu Lys Glu Pro Leu Phe Phe Ser Leu Thr His Asp Thr 
            340                 345                 350         


Lys Leu Gln Val Gln Glu Asn Lys Glu Asn Tyr Lys Lys Pro Tyr Ser 
        355                 360                 365             


Val Glu Gln Ile Leu Glu Gln Ala Lys Leu Ala Gly Asp Lys Tyr Phe 
    370                 375                 380                 


Lys Gly Lys Ser Asn Asp Gly Ile Leu Asn Thr Ile Pro Glu Phe Ser 
385                 390                 395                 400 


Asp Tyr Ile Leu Gln Lys Glu Ser Tyr Gly Gly Val Tyr Trp Ser Lys 
                405                 410                 415     


Val Ala Ile Asn Thr Ile Ser Asn Arg Tyr Phe Glu Asn Trp His Thr 
            420                 425                 430         


Leu Lys Asp Ala Leu Ile Asp Ala Lys Val Phe Glu Lys Gly Lys Lys 
        435                 440                 445             


Gly Asp Glu Glu Asp Val Lys Ile Pro Glu Ser Ile Glu Leu Gln Gly 
    450                 455                 460                 


Phe Phe Asp Val Leu Asp Thr Gln Glu Gly Trp Lys Ser Glu Gly Ile 
465                 470                 475                 480 


Phe Phe Lys Lys Ser Leu Thr Glu Glu Leu Lys Asn Lys Glu Ala Asp 
                485                 490                 495     


Thr Lys Asn Lys Lys Arg Arg Glu Ile Val Lys Glu Ala Lys Arg Pro 
            500                 505                 510         


Ser Gln Ala Leu Leu Ala Leu Leu Phe Asp Asp Ile Gln Glu His Ala 
        515                 520                 525             


Asn Leu Phe Leu Asn Gln Ser Asp Glu Ile Leu Lys Leu Glu Lys Tyr 
    530                 535                 540                 


Ser Glu Lys Glu Ser Lys Glu Lys Ile Lys Gln Trp Met Asp Ser Ala 
545                 550                 555                 560 


Leu Ala Val Asn Arg Ile Leu Lys Tyr Phe Phe Val Lys Glu Ser Lys 
                565                 570                 575     


Ala Lys Gly Glu Gln Ile Asp Pro Ile Ile Ser Glu Ala Leu Lys Val 
            580                 585                 590         


Leu Phe Asn Ser Glu Asp Ala Thr Trp Phe Lys Trp Tyr Asp Ala Leu 
        595                 600                 605             


Arg Asn Tyr Leu Thr Lys Lys Pro Gln Asp Asp Val Lys Glu Asn Lys 
    610                 615                 620                 


Leu Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Gly Gly Trp Asp Ile 
625                 630                 635                 640 


Asn Lys Glu Ser Asp Asn Tyr Cys Val Ile Leu Gln Asp Gln Lys Gly 
                645                 650                 655     


Lys Gln His Leu Gly Ile Ile Ala Lys Lys Glu Lys Gln Arg Gly Tyr 
            660                 665                 670         


Asn Lys Ile Phe Glu Arg Thr Pro Glu Asn Pro Leu Tyr Lys Ile Asp 
        675                 680                 685             


Ser Gly Glu Val Trp Gln Lys Met Glu Tyr Lys Gln Ile Ala Val Pro 
    690                 695                 700                 


Thr Gly Ile Gly Gly Phe Val Arg Lys Cys Phe Asn Thr Ala Gln Gln 
705                 710                 715                 720 


Tyr Gly Trp Lys Cys Pro Asp Asn Cys Leu Asn Ser Glu Gly Lys Ile 
                725                 730                 735     


Ile Ile Lys Asn Asp Glu Ala Lys Glu Asn Leu Glu Ala Ile Ile Asp 
            740                 745                 750         


Cys Tyr Lys Asp Phe Phe Thr Lys Tyr Glu Lys Asp Gly Phe Lys Tyr 
        755                 760                 765             


Lys Asn Phe Asn Phe Asn Phe Lys Glu Ser Glu Glu Tyr Glu Glu Leu 
    770                 775                 780                 


Asn Ser Phe Phe Ser Asp Val Glu Arg Gln Gly Tyr Lys Leu Gly Phe 
785                 790                 795                 800 


Ile Thr Val Asn Lys Thr Ile Ile Asp Lys Trp Val Glu Asp Gly Thr 
                805                 810                 815     


Ile Tyr Leu Phe Glu Ile Lys Asn Gln Asp Thr Asn Asp Gly Lys Lys 
            820                 825                 830         


Asp Gly His Lys Asn Asn Leu His Thr Ile Tyr Trp Lys Ala Leu Phe 
        835                 840                 845             


Glu Asp Val Gly Asn Lys Pro Lys Leu Asn Gly Gly Ala Glu Leu Phe 
    850                 855                 860                 


Tyr Arg Lys Ala Leu Leu Lys Asp Lys Gln Glu Lys Ile Glu Asp Asn 
865                 870                 875                 880 


Tyr Gly Lys Glu Ile Ile Lys Asn Phe Arg Phe Ser Lys Glu Lys Phe 
                885                 890                 895     


Leu Phe His Cys Pro Ile Lys Leu Asn Tyr Lys Ala Lys Ser Tyr Ser 
            900                 905                 910         


His Pro Lys Tyr Ala Leu Pro Glu Ile Asn Lys Gln Thr Asn Glu Phe 
        915                 920                 925             


Phe Ile Thr Ile Ser Asp Ile Cys Phe Leu Gly Ile Asp Arg Gly Glu 
    930                 935                 940                 


Lys His Leu Ala Tyr Tyr Ser Leu Val Asn Gln Lys Glu Glu Ile Ile 
945                 950                 955                 960 


Asp Gln Gly Thr Leu Asn Met Pro Phe Val Asp Lys Asp Gly Lys Pro 
                965                 970                 975     


Arg Ser Ile Lys Lys Glu Lys Phe Arg Phe Asp Ala Lys Val Asp Lys 
            980                 985                 990         


Trp Val Ser Glu Glu Met Asp Cys  Trp Asn Tyr Asn Asp  Leu Leu Asp 
        995                 1000                 1005             


Ala Met  Ala Ser Asn Arg Asp  Met Ala Arg Lys Asn  Trp Gln Thr 
    1010                 1015                 1020             


Ile Gly  Thr Ile Lys Glu Leu  Lys Asn Gly Tyr Ile  Ser Gln Val 
    1025                 1030                 1035             


Val Arg  Lys Ile Val Asp Leu  Ala Ile Asp Lys Asn  Arg Gly Ile 
    1040                 1045                 1050             


Pro Ser  Phe Ile Val Leu Glu  Asn Leu Asn Ala Gly  Phe Met Arg 
    1055                 1060                 1065             


Gly Arg  Gln Lys Ile Glu Lys  Ser Val Tyr Gln Lys  Leu Glu Leu 
    1070                 1075                 1080             


Ala Leu  Ala Lys Lys Leu Asn  Phe Val Val Asp Lys  Thr Val Gln 
    1085                 1090                 1095             


Asn Gly  Glu Ile Gly Ser Val  Thr Asn Ala Leu Gln  Leu Thr Pro 
    1100                 1105                 1110             


Pro Val  Thr Asn Phe Gln Asn  Ile Glu Asn Arg Lys  Gln Val Gly 
    1115                 1120                 1125             


Ile Met  Leu Tyr Thr Arg Ala  Asp Tyr Thr Ser Gln  Thr Asp Pro 
    1130                 1135                 1140             


Val Thr  Gly Trp Arg Lys Ser  Ile Tyr Leu Lys Lys  Gly Ser Glu 
    1145                 1150                 1155             


Glu Tyr  Ile Lys Ser Gln Ile  Met Asp Ser Phe Asp  Asp Phe Gly 
    1160                 1165                 1170             


Phe Asp  Gly Lys Asn Tyr Tyr  Phe Lys Tyr Thr Val  Asp Leu Ile 
    1175                 1180                 1185             


Asp Pro  Ile Lys Lys Thr Ser  Glu Lys Lys Phe Lys  Lys Gln Trp 
    1190                 1195                 1200             


Thr Leu  Tyr Ser Gly Ile Asn  Gly Lys Ser Leu Asp  Arg Phe Arg 
    1205                 1210                 1215             


Gly Glu  Arg Ser Ser Asp Lys  Asn Val Trp Ile Lys  Thr Leu Gln 
    1220                 1225                 1230             


Asp Ile  Lys Thr Thr Leu Asp  Ala Val Phe Ile Gly  Phe Asp Leu 
    1235                 1240                 1245             


Gln His  Ser Leu Arg Ser Gln  Ile Ile Asn Asp Gly  Ile Met Pro 
    1250                 1255                 1260             


Lys Lys  Ile Glu Asn Ser Gln  Phe Thr Gly Trp Glu  Ser Leu Arg 
    1265                 1270                 1275             


Phe Ala  Ile Glu Met Ile Gln  Gln Ile Arg Asn Ile  Gly Val Glu 
    1280                 1285                 1290             


Lys Gly  Asp Glu Asp Phe Ile  Leu Ser Pro Val Arg  Asp Lys Asn 
    1295                 1300                 1305             


Glu Asn  His Phe Asp Ser Arg  Lys Ala Lys Glu Asn  Glu Pro Asn 
    1310                 1315                 1320             


Ser Gly  Asp Ala Asn Gly Ala  Tyr Asn Ile Ala Arg  Lys Gly Ile 
    1325                 1330                 1335             


Ile Met  Asn Glu His Ile Lys  Lys Asp Leu Ile Pro  Phe Ile Gln 
    1340                 1345                 1350             


Asp Lys  Glu Trp Asp Ala Trp  Leu Ala Gly Arg Glu  Val Trp Asp 
    1355                 1360                 1365             


Lys Trp  Leu Ile Glu Asn Lys  Asp Ser Leu Ile Tyr  Lys Lys 
    1370                 1375                 1380         


<210>  1362
<211>  1400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1362

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
            20                  25                  30          


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
        35                  40                  45              


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
    50                  55                  60                  


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
65                  70                  75                  80  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
                85                  90                  95      


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
            100                 105                 110         


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
        115                 120                 125             


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
    130                 135                 140                 


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
145                 150                 155                 160 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
                165                 170                 175     


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
            180                 185                 190         


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
        195                 200                 205             


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
    210                 215                 220                 


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
225                 230                 235                 240 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
                245                 250                 255     


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
            260                 265                 270         


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
        275                 280                 285             


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
    290                 295                 300                 


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
305                 310                 315                 320 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
                325                 330                 335     


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
            340                 345                 350         


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
        355                 360                 365             


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
    370                 375                 380                 


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
385                 390                 395                 400 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
                405                 410                 415     


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
            420                 425                 430         


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
        435                 440                 445             


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
465                 470                 475                 480 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
                485                 490                 495     


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
            500                 505                 510         


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
        515                 520                 525             


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
    530                 535                 540                 


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
545                 550                 555                 560 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
                565                 570                 575     


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
            580                 585                 590         


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
        595                 600                 605             


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
    610                 615                 620                 


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
625                 630                 635                 640 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
                645                 650                 655     


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
            660                 665                 670         


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
        675                 680                 685             


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
    690                 695                 700                 


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
705                 710                 715                 720 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
                725                 730                 735     


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
            740                 745                 750         


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
        755                 760                 765             


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
    770                 775                 780                 


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
785                 790                 795                 800 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
                805                 810                 815     


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
            820                 825                 830         


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
        835                 840                 845             


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
    850                 855                 860                 


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
865                 870                 875                 880 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
                885                 890                 895     


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
            900                 905                 910         


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
        915                 920                 925             


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
    930                 935                 940                 


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
945                 950                 955                 960 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
                965                 970                 975     


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
            980                 985                 990         


Glu Ile Asn Asn Tyr His His Ala  His Asp Ala Tyr Leu  Asn Ala Val 
        995                 1000                 1005             


Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu 
    1010                 1015                 1020             


Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile 
    1025                 1030                 1035             


Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala Thr Ala  Lys Tyr Phe 
    1040                 1045                 1050             


Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr Glu  Ile Thr Leu 
    1055                 1060                 1065             


Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  Thr Asn Gly 
    1070                 1075                 1080             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr 
    1085                 1090                 1095             


Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys Lys 
    1100                 1105                 1110             


Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro 
    1115                 1120                 1125             


Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp 
    1130                 1135                 1140             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser 
    1145                 1150                 1155             


Val Leu  Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu 
    1160                 1165                 1170             


Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1175                 1180                 1185             


Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr 
    1190                 1195                 1200             


Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser 
    1205                 1210                 1215             


Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala 
    1220                 1225                 1230             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr 
    1235                 1240                 1245             


Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly 
    1250                 1255                 1260             


Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu Phe Val  Glu Gln His 
    1265                 1270                 1275             


Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser 
    1280                 1285                 1290             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser 
    1295                 1300                 1305             


Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu 
    1310                 1315                 1320             


Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala 
    1325                 1330                 1335             


Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr 
    1340                 1345                 1350             


Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
    1355                 1360                 1365             


Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly 
    1370                 1375                 1380             


Asp Lys  Arg Pro Ala Ala Thr  Lys Lys Ala Gly Gln  Ala Lys Lys 
    1385                 1390                 1395             


Lys Lys  
    1400 


<210>  1363
<211>  1661
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1363

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Asn Phe Lys Ile Leu Pro Ile Ala Ile Asp Leu Gly Val Lys Asn 
            20                  25                  30          


Thr Gly Val Phe Ser Ala Phe Tyr Gln Lys Gly Thr Ser Leu Glu Arg 
        35                  40                  45              


Leu Asp Asn Lys Asn Gly Lys Val Tyr Glu Leu Ser Lys Asp Ser Tyr 
    50                  55                  60                  


Thr Leu Leu Met Asn Asn Arg Thr Ala Arg Arg His Gln Arg Arg Gly 
65                  70                  75                  80  


Ile Asp Arg Lys Gln Leu Val Lys Arg Leu Phe Lys Leu Ile Trp Thr 
                85                  90                  95      


Glu Gln Leu Asn Leu Glu Trp Asp Lys Asp Thr Gln Gln Ala Ile Ser 
            100                 105                 110         


Phe Leu Phe Asn Arg Arg Gly Phe Ser Phe Ile Thr Asp Gly Tyr Ser 
        115                 120                 125             


Pro Glu Tyr Leu Asn Ile Val Pro Glu Gln Val Lys Ala Ile Leu Met 
    130                 135                 140                 


Asp Ile Phe Asp Asp Tyr Asn Gly Glu Asp Asp Leu Asp Ser Tyr Leu 
145                 150                 155                 160 


Lys Leu Ala Thr Glu Gln Glu Ser Lys Ile Ser Glu Ile Tyr Asn Lys 
                165                 170                 175     


Leu Met Gln Lys Ile Leu Glu Phe Lys Leu Met Lys Leu Cys Thr Asp 
            180                 185                 190         


Ile Lys Asp Asp Lys Val Ser Thr Lys Thr Leu Lys Glu Ile Thr Ser 
        195                 200                 205             


Tyr Glu Phe Glu Leu Leu Ala Asp Tyr Leu Ala Asn Tyr Ser Glu Ser 
    210                 215                 220                 


Leu Lys Thr Gln Lys Phe Ser Tyr Thr Asp Lys Gln Gly Asn Leu Lys 
225                 230                 235                 240 


Glu Leu Ser Tyr Tyr His His Asp Lys Tyr Asn Ile Gln Glu Phe Leu 
                245                 250                 255     


Lys Arg His Ala Thr Ile Asn Asp Arg Ile Leu Asp Thr Leu Leu Thr 
            260                 265                 270         


Asp Asp Leu Asp Ile Trp Asn Phe Asn Phe Glu Lys Phe Asp Phe Asp 
        275                 280                 285             


Lys Asn Glu Glu Lys Leu Gln Asn Gln Glu Asp Lys Asp His Ile Gln 
    290                 295                 300                 


Ala His Leu His His Phe Val Phe Ala Val Asn Lys Ile Lys Ser Glu 
305                 310                 315                 320 


Met Ala Ser Gly Gly Arg His Arg Ser Gln Tyr Phe Gln Glu Ile Thr 
                325                 330                 335     


Asn Val Leu Asp Glu Asn Asn His Gln Glu Gly Tyr Leu Lys Asn Phe 
            340                 345                 350         


Cys Glu Asn Leu His Asn Lys Lys Tyr Ser Asn Leu Ser Val Lys Asn 
        355                 360                 365             


Leu Val Asn Leu Ile Gly Asn Leu Ser Asn Leu Glu Leu Lys Pro Leu 
    370                 375                 380                 


Arg Lys Tyr Phe Asn Asp Lys Ile His Ala Lys Ala Asp His Trp Asp 
385                 390                 395                 400 


Glu Gln Lys Phe Thr Glu Thr Tyr Cys His Trp Ile Leu Gly Glu Trp 
                405                 410                 415     


Arg Val Gly Val Lys Asp Gln Asp Lys Lys Asp Gly Ala Lys Tyr Ser 
            420                 425                 430         


Tyr Lys Asp Leu Cys Asn Glu Leu Lys Gln Lys Val Thr Lys Ala Gly 
        435                 440                 445             


Leu Val Asp Phe Leu Leu Glu Leu Asp Pro Cys Arg Thr Ile Pro Pro 
    450                 455                 460                 


Tyr Leu Asp Asn Asn Asn Arg Lys Pro Pro Lys Cys Gln Ser Leu Ile 
465                 470                 475                 480 


Leu Asn Pro Lys Phe Leu Asp Asn Gln Tyr Pro Asn Trp Gln Gln Tyr 
                485                 490                 495     


Leu Gln Glu Leu Lys Lys Leu Gln Ser Ile Gln Asn Tyr Leu Asp Ser 
            500                 505                 510         


Phe Glu Thr Asp Leu Lys Val Leu Lys Ser Ser Lys Asp Gln Pro Tyr 
        515                 520                 525             


Phe Val Glu Tyr Lys Ser Ser Asn Gln Gln Ile Ala Ser Gly Gln Arg 
    530                 535                 540                 


Asp Tyr Lys Asp Leu Asp Ala Arg Ile Leu Gln Phe Ile Phe Asp Arg 
545                 550                 555                 560 


Val Lys Ala Ser Asp Glu Leu Leu Leu Asn Glu Ile Tyr Phe Gln Ala 
                565                 570                 575     


Lys Lys Leu Lys Gln Lys Ala Ser Ser Glu Leu Glu Lys Leu Glu Ser 
            580                 585                 590         


Ser Lys Lys Leu Asp Glu Val Ile Ala Asn Ser Gln Leu Ser Gln Ile 
        595                 600                 605             


Leu Lys Ser Gln His Thr Asn Gly Ile Phe Glu Gln Gly Thr Phe Leu 
    610                 615                 620                 


His Leu Val Cys Lys Tyr Tyr Lys Gln Arg Gln Arg Ala Arg Asp Ser 
625                 630                 635                 640 


Arg Leu Tyr Ile Met Pro Glu Tyr Arg Tyr Asp Lys Lys Leu His Lys 
                645                 650                 655     


Tyr Asn Asn Thr Gly Arg Phe Asp Asp Asp Asn Gln Leu Leu Thr Tyr 
            660                 665                 670         


Cys Asn His Lys Pro Arg Gln Lys Arg Tyr Gln Leu Leu Asn Asp Leu 
        675                 680                 685             


Ala Gly Val Leu Gln Val Ser Pro Asn Phe Leu Lys Asp Lys Ile Gly 
    690                 695                 700                 


Ser Asp Asp Asp Leu Phe Ile Ser Lys Trp Leu Val Glu His Ile Arg 
705                 710                 715                 720 


Gly Phe Lys Lys Ala Cys Glu Asp Ser Leu Lys Ile Gln Lys Asp Asn 
                725                 730                 735     


Arg Gly Leu Leu Asn His Lys Ile Asn Ile Ala Arg Asn Thr Lys Gly 
            740                 745                 750         


Lys Cys Glu Lys Glu Ile Phe Asn Leu Ile Cys Lys Ile Glu Gly Ser 
        755                 760                 765             


Glu Asp Lys Lys Gly Asn Tyr Lys His Gly Leu Ala Tyr Glu Leu Gly 
    770                 775                 780                 


Val Leu Leu Phe Gly Glu Pro Asn Glu Ala Ser Lys Pro Glu Phe Asp 
785                 790                 795                 800 


Arg Lys Ile Lys Lys Phe Asn Ser Ile Tyr Ser Phe Ala Gln Ile Gln 
                805                 810                 815     


Gln Ile Ala Phe Ala Glu Arg Lys Gly Asn Ala Asn Thr Cys Ala Val 
            820                 825                 830         


Cys Ser Ala Asp Asn Ala His Arg Met Gln Gln Ile Lys Ile Thr Glu 
        835                 840                 845             


Pro Val Glu Asp Asn Lys Asp Lys Ile Ile Leu Ser Ala Lys Ala Gln 
    850                 855                 860                 


Arg Leu Pro Ala Ile Pro Thr Arg Ile Val Asp Gly Ala Val Lys Lys 
865                 870                 875                 880 


Met Ala Thr Ile Leu Ala Lys Asn Ile Val Asp Asp Asn Trp Gln Asn 
                885                 890                 895     


Ile Lys Gln Val Leu Ser Ala Lys His Gln Leu His Ile Pro Ile Ile 
            900                 905                 910         


Thr Glu Ser Asn Ala Phe Glu Phe Glu Pro Ala Leu Ala Asp Val Lys 
        915                 920                 925             


Gly Lys Ser Leu Lys Asp Arg Arg Lys Lys Ala Leu Glu Arg Ile Ser 
    930                 935                 940                 


Pro Glu Asn Ile Phe Lys Asp Lys Asn Asn Arg Ile Lys Glu Phe Ala 
945                 950                 955                 960 


Lys Gly Ile Ser Ala Tyr Ser Gly Ala Asn Leu Thr Asp Gly Asp Phe 
                965                 970                 975     


Asp Gly Ala Lys Glu Glu Leu Asp His Ile Ile Pro Arg Ser His Lys 
            980                 985                 990         


Lys Tyr Gly Thr Leu Asn Asp Glu  Ala Asn Leu Ile Cys  Val Thr Arg 
        995                 1000                 1005             


Gly Asp  Asn Lys Asn Lys Gly  Asn Arg Ile Phe Cys  Leu Arg Asp 
    1010                 1015                 1020             


Leu Ala  Asp Asn Tyr Lys Leu  Lys Gln Phe Glu Thr  Thr Asp Asp 
    1025                 1030                 1035             


Leu Glu  Ile Glu Lys Lys Ile  Ala Asp Thr Ile Trp  Asp Ala Asn 
    1040                 1045                 1050             


Lys Lys  Asp Phe Lys Phe Gly  Asn Tyr Arg Ser Phe  Ile Asn Leu 
    1055                 1060                 1065             


Thr Pro  Gln Glu Gln Lys Ala  Phe Arg His Ala Leu  Phe Leu Ala 
    1070                 1075                 1080             


Asp Glu  Asn Pro Ile Lys Gln  Ala Val Ile Arg Ala  Ile Asn Asn 
    1085                 1090                 1095             


Arg Asn  Arg Thr Phe Val Asn  Gly Thr Gln Arg Tyr  Phe Ala Glu 
    1100                 1105                 1110             


Val Leu  Ala Asn Asn Ile Tyr  Leu Arg Ala Lys Lys  Glu Asn Leu 
    1115                 1120                 1125             


Asn Thr  Asp Lys Ile Ser Phe  Asp Tyr Phe Gly Ile  Pro Thr Ile 
    1130                 1135                 1140             


Gly Asn  Gly Arg Gly Ile Ala  Glu Ile Arg Gln Leu  Tyr Glu Lys 
    1145                 1150                 1155             


Val Asp  Ser Asp Ile Gln Ala  Tyr Ala Lys Gly Asp  Lys Pro Gln 
    1160                 1165                 1170             


Ala Ser  Tyr Ser His Leu Ile  Asp Ala Met Leu Ala  Phe Cys Ile 
    1175                 1180                 1185             


Ala Ala  Asp Glu His Arg Asn  Asp Gly Ser Ile Gly  Leu Glu Ile 
    1190                 1195                 1200             


Asp Lys  Asn Tyr Ser Leu Tyr  Pro Leu Asp Lys Asn  Thr Gly Glu 
    1205                 1210                 1215             


Val Phe  Thr Lys Asp Ile Phe  Ser Gln Ile Lys Ile  Thr Asp Asn 
    1220                 1225                 1230             


Glu Phe  Ser Asp Lys Lys Leu  Val Arg Lys Lys Ala  Ile Glu Gly 
    1235                 1240                 1245             


Phe Asn  Thr His Arg Gln Met  Thr Arg Asp Gly Ile  Tyr Ala Glu 
    1250                 1255                 1260             


Asn Tyr  Leu Pro Ile Leu Ile  His Lys Glu Leu Asn  Glu Val Arg 
    1265                 1270                 1275             


Lys Gly  Tyr Thr Trp Lys Asn  Ser Glu Glu Ile Lys  Ile Phe Lys 
    1280                 1285                 1290             


Gly Lys  Lys Tyr Asp Ile Gln  Gln Leu Asn Asn Leu  Val Tyr Cys 
    1295                 1300                 1305             


Leu Lys  Phe Val Asp Lys Pro  Ile Ser Ile Asp Ile  Gln Ile Ser 
    1310                 1315                 1320             


Thr Leu  Glu Glu Leu Arg Asn  Ile Leu Thr Thr Asn  Asn Ile Ala 
    1325                 1330                 1335             


Ala Thr  Ala Glu Tyr Tyr Tyr  Ile Asn Leu Lys Thr  Gln Lys Leu 
    1340                 1345                 1350             


His Glu  Tyr Tyr Ile Glu Asn  Tyr Asn Thr Ala Leu  Gly Tyr Lys 
    1355                 1360                 1365             


Lys Tyr  Ser Lys Glu Met Glu  Phe Leu Arg Ser Leu  Ala Tyr Arg 
    1370                 1375                 1380             


Ser Glu  Arg Val Lys Ile Lys  Ser Ile Asp Asp Val  Lys Gln Val 
    1385                 1390                 1395             


Leu Asp  Lys Asp Ser Asn Phe  Ile Ile Gly Lys Ile  Thr Leu Pro 
    1400                 1405                 1410             


Phe Lys  Lys Glu Trp Gln Arg  Leu Tyr Arg Glu Trp  Gln Asn Thr 
    1415                 1420                 1425             


Thr Ile  Lys Asp Asp Tyr Glu  Phe Leu Lys Ser Phe  Phe Asn Val 
    1430                 1435                 1440             


Lys Ser  Ile Thr Lys Leu His  Lys Lys Val Arg Lys  Asp Phe Ser 
    1445                 1450                 1455             


Leu Pro  Ile Ser Thr Asn Glu  Gly Lys Phe Leu Val  Lys Arg Lys 
    1460                 1465                 1470             


Thr Trp  Asp Asn Asn Phe Ile  Tyr Gln Ile Leu Asn  Asp Ser Asp 
    1475                 1480                 1485             


Ser Arg  Ala Asp Gly Thr Lys  Pro Phe Ile Pro Ala  Phe Asp Ile 
    1490                 1495                 1500             


Ser Lys  Asn Glu Ile Val Glu  Ala Ile Ile Asp Ser  Phe Thr Ser 
    1505                 1510                 1515             


Lys Asn  Ile Phe Trp Leu Pro  Lys Asn Ile Glu Leu  Gln Lys Val 
    1520                 1525                 1530             


Asp Asn  Lys Asn Ile Phe Ala  Ile Asp Thr Ser Lys  Trp Phe Glu 
    1535                 1540                 1545             


Val Glu  Thr Pro Ser Asp Leu  Arg Asp Ile Gly Ile  Ala Thr Ile 
    1550                 1555                 1560             


Gln Tyr  Lys Ile Asp Asn Asn  Ser Arg Pro Lys Val  Arg Val Lys 
    1565                 1570                 1575             


Leu Asp  Tyr Val Ile Asp Asp  Asp Ser Lys Ile Asn  Tyr Phe Met 
    1580                 1585                 1590             


Asn His  Ser Leu Leu Lys Ser  Arg Tyr Pro Asp Lys  Val Leu Glu 
    1595                 1600                 1605             


Ile Leu  Lys Gln Ser Thr Ile  Ile Glu Phe Glu Ser  Ser Gly Phe 
    1610                 1615                 1620             


Asn Lys  Thr Ile Lys Glu Met  Leu Gly Met Lys Leu  Ala Gly Ile 
    1625                 1630                 1635             


Tyr Asn  Glu Thr Ser Asn Asn  Lys Arg Pro Ala Ala  Thr Lys Lys 
    1640                 1645                 1650             


Ala Gly  Gln Ala Lys Lys Lys  Lys 
    1655                 1660     


<210>  1364
<211>  1085
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1364

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
            20                  25                  30          


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
        35                  40                  45              


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
    50                  55                  60                  


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
65                  70                  75                  80  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
                85                  90                  95      


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
            100                 105                 110         


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
        115                 120                 125             


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
    130                 135                 140                 


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
145                 150                 155                 160 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
                165                 170                 175     


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
            180                 185                 190         


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
        195                 200                 205             


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
    210                 215                 220                 


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
225                 230                 235                 240 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
                245                 250                 255     


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
            260                 265                 270         


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
        275                 280                 285             


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
    290                 295                 300                 


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
305                 310                 315                 320 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
                325                 330                 335     


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
            340                 345                 350         


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
        355                 360                 365             


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
    370                 375                 380                 


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
385                 390                 395                 400 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
                405                 410                 415     


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
            420                 425                 430         


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
        435                 440                 445             


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
    450                 455                 460                 


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
465                 470                 475                 480 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
                485                 490                 495     


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
            500                 505                 510         


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
        515                 520                 525             


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
    530                 535                 540                 


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
545                 550                 555                 560 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
                565                 570                 575     


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
            580                 585                 590         


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
        595                 600                 605             


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
    610                 615                 620                 


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
625                 630                 635                 640 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
                645                 650                 655     


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
            660                 665                 670         


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
        675                 680                 685             


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
    690                 695                 700                 


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
705                 710                 715                 720 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
                725                 730                 735     


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
            740                 745                 750         


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
        755                 760                 765             


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
    770                 775                 780                 


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
785                 790                 795                 800 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
                805                 810                 815     


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
            820                 825                 830         


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
        835                 840                 845             


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
    850                 855                 860                 


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
865                 870                 875                 880 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
                885                 890                 895     


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
            900                 905                 910         


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
        915                 920                 925             


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
    930                 935                 940                 


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
945                 950                 955                 960 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
                965                 970                 975     


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
            980                 985                 990         


Glu Leu Tyr Arg Val Ile Gly Val  Asn Asn Asp Leu Leu  Asn Arg Ile 
        995                 1000                 1005             


Glu Val  Asn Met Ile Asp Ile  Thr Tyr Arg Glu Tyr  Leu Glu Asn 
    1010                 1015                 1020             


Met Asn  Asp Lys Arg Pro Pro  Arg Ile Ile Lys Thr  Ile Ala Ser 
    1025                 1030                 1035             


Lys Thr  Gln Ser Ile Lys Lys  Tyr Ser Thr Asp Ile  Leu Gly Asn 
    1040                 1045                 1050             


Leu Tyr  Glu Val Lys Ser Lys  Lys His Pro Gln Ile  Ile Lys Lys 
    1055                 1060                 1065             


Gly Lys  Arg Pro Ala Ala Thr  Lys Lys Ala Gly Gln  Ala Lys Lys 
    1070                 1075                 1080             


Lys Lys  
    1085 


<210>  1365
<211>  1441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1365

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Leu Phe Asn Lys Cys Ile Ile Ile Ser Ile Asn Leu Asp Phe Ser 
            20                  25                  30          


Asn Lys Glu Lys Cys Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile 
        35                  40                  45              


Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Asn Tyr Lys Val 
    50                  55                  60                  


Pro Ser Lys Lys Met Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile 
65                  70                  75                  80  


Lys Lys Asn Leu Leu Gly Val Leu Leu Phe Asp Ser Gly Ile Thr Ala 
                85                  90                  95      


Glu Gly Arg Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg 
            100                 105                 110         


Arg Asn Arg Ile Leu Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala 
        115                 120                 125             


Thr Leu Asp Asp Ala Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val 
    130                 135                 140                 


Pro Asp Asp Lys Arg Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val 
145                 150                 155                 160 


Glu Glu Lys Val Tyr His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg 
                165                 170                 175     


Lys Tyr Leu Ala Asp Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr 
            180                 185                 190         


Leu Ala Leu Ala His Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu 
        195                 200                 205             


Gly Glu Phe Asn Ser Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp 
    210                 215                 220                 


Phe Leu Asp Thr Tyr Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu 
225                 230                 235                 240 


Asn Ser Lys Gln Leu Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu 
                245                 250                 255     


Glu Lys Lys Asp Arg Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser 
            260                 265                 270         


Gly Ile Phe Ser Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp 
        275                 280                 285             


Phe Arg Lys Cys Phe Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser 
    290                 295                 300                 


Lys Glu Ser Tyr Asp Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly 
305                 310                 315                 320 


Asp Asp Tyr Ser Asp Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala 
                325                 330                 335     


Ile Leu Leu Ser Gly Phe Leu Thr Val Thr Asp Asn Glu Thr Glu Ala 
            340                 345                 350         


Pro Leu Ser Ser Ala Met Ile Lys Arg Tyr Asn Glu His Lys Glu Asp 
        355                 360                 365             


Leu Ala Leu Leu Lys Glu Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr 
    370                 375                 380                 


Asn Glu Val Phe Lys Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile 
385                 390                 395                 400 


Asp Gly Lys Thr Asn Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu 
                405                 410                 415     


Leu Ala Glu Phe Glu Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg 
            420                 425                 430         


Glu Asp Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro 
        435                 440                 445             


Tyr Gln Ile His Leu Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala 
    450                 455                 460                 


Lys Phe Tyr Pro Phe Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile 
465                 470                 475                 480 


Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn 
                485                 490                 495     


Ser Asp Phe Ala Trp Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro 
            500                 505                 510         


Trp Asn Phe Glu Asp Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe 
        515                 520                 525             


Ile Asn Arg Met Thr Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val 
    530                 535                 540                 


Leu Pro Lys His Ser Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu 
545                 550                 555                 560 


Leu Thr Lys Val Arg Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe 
                565                 570                 575     


Leu Asp Ser Lys Gln Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp 
            580                 585                 590         


Lys Arg Lys Val Thr Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile 
        595                 600                 605             


Tyr Gly Tyr Asp Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn 
    610                 615                 620                 


Ser Ser Leu Ser Thr Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys 
625                 630                 635                 640 


Glu Phe Leu Asp Asp Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile 
                645                 650                 655     


His Thr Leu Thr Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu 
            660                 665                 670         


Ser Lys Phe Glu Asn Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser 
        675                 680                 685             


Arg Arg His Tyr Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn 
    690                 695                 700                 


Gly Ile Arg Asp Glu Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile 
705                 710                 715                 720 


Asp Asp Gly Ile Ser Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp 
                725                 730                 735     


Ala Leu Ser Phe Lys Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp 
            740                 745                 750         


Glu Asp Lys Gly Asn Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser 
        755                 760                 765             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu 
    770                 775                 780                 


Leu Val Lys Val Met Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu 
785                 790                 795                 800 


Met Ala Arg Glu Asn Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln 
                805                 810                 815     


Gln Arg Leu Lys Arg Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys 
            820                 825                 830         


Ile Leu Lys Glu Asn Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn 
        835                 840                 845             


Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys 
    850                 855                 860                 


Asp Met Tyr Thr Gly Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr 
865                 870                 875                 880 


Asp Ile Asp His Ile Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile 
                885                 890                 895     


Asp Asn Lys Val Leu Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp 
            900                 905                 910         


Asp Phe Pro Ser Leu Glu Val Val Lys Lys Arg Lys Thr Phe Trp Tyr 
        915                 920                 925             


Gln Leu Leu Lys Ser Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu 
    930                 935                 940                 


Thr Lys Ala Glu Arg Gly Gly Leu Leu Pro Glu Asp Lys Ala Gly Phe 
945                 950                 955                 960 


Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala 
                965                 970                 975     


Arg Leu Leu Asp Glu Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg 
            980                 985                 990         


Ala Val Arg Thr Val Lys Ile Ile  Thr Leu Lys Ser Thr  Leu Val Ser 
        995                 1000                 1005             


Gln Phe  Arg Lys Asp Phe Glu  Leu Tyr Lys Val Arg  Glu Ile Asn 
    1010                 1015                 1020             


Asp Phe  His His Ala His Asp  Ala Tyr Leu Asn Ala  Val Ile Ala 
    1025                 1030                 1035             


Ser Ala  Leu Leu Lys Lys Tyr  Pro Lys Leu Glu Pro  Glu Phe Val 
    1040                 1045                 1050             


Tyr Gly  Asp Tyr Pro Lys Tyr  Asn Ser Phe Arg Glu  Arg Lys Ser 
    1055                 1060                 1065             


Ala Thr  Glu Lys Val Tyr Phe  Tyr Ser Asn Ile Met  Asn Ile Phe 
    1070                 1075                 1080             


Lys Lys  Ser Ile Ser Leu Ala  Asp Gly Arg Val Ile  Glu Arg Pro 
    1085                 1090                 1095             


Leu Ile  Glu Val Asn Glu Glu  Thr Gly Glu Ser Val  Trp Asn Lys 
    1100                 1105                 1110             


Glu Ser  Asp Leu Ala Thr Val  Arg Arg Val Leu Ser  Tyr Pro Gln 
    1115                 1120                 1125             


Val Asn  Val Val Lys Lys Val  Glu Glu Gln Asn His  Gly Leu Asp 
    1130                 1135                 1140             


Arg Gly  Lys Pro Lys Gly Leu  Phe Asn Ala Asn Leu  Ser Ser Lys 
    1145                 1150                 1155             


Pro Lys  Pro Asn Ser Asn Glu  Asn Leu Val Gly Ala  Lys Glu Tyr 
    1160                 1165                 1170             


Leu Asp  Pro Lys Lys Tyr Gly  Gly Tyr Ala Gly Ile  Ser Asn Ser 
    1175                 1180                 1185             


Phe Ala  Val Leu Val Lys Gly  Thr Ile Glu Lys Gly  Ala Lys Lys 
    1190                 1195                 1200             


Lys Ile  Thr Asn Val Leu Glu  Phe Gln Gly Ile Ser  Ile Leu Asp 
    1205                 1210                 1215             


Arg Ile  Asn Tyr Arg Lys Asp  Lys Leu Asn Phe Leu  Leu Glu Lys 
    1220                 1225                 1230             


Gly Tyr  Lys Asp Ile Glu Leu  Ile Ile Glu Leu Pro  Lys Tyr Ser 
    1235                 1240                 1245             


Leu Phe  Glu Leu Ser Asp Gly  Ser Arg Arg Met Leu  Ala Ser Ile 
    1250                 1255                 1260             


Leu Ser  Thr Asn Asn Lys Arg  Gly Glu Ile His Lys  Gly Asn Gln 
    1265                 1270                 1275             


Ile Phe  Leu Ser Gln Lys Phe  Val Lys Leu Leu Tyr  His Ala Lys 
    1280                 1285                 1290             


Arg Ile  Ser Asn Thr Ile Asn  Glu Asn His Arg Lys  Tyr Val Glu 
    1295                 1300                 1305             


Asn His  Lys Lys Glu Phe Glu  Glu Leu Phe Tyr Tyr  Ile Leu Glu 
    1310                 1315                 1320             


Phe Asn  Glu Asn Tyr Val Gly  Ala Lys Lys Asn Gly  Lys Leu Leu 
    1325                 1330                 1335             


Asn Ser  Ala Phe Gln Ser Trp  Gln Asn His Ser Ile  Asp Glu Leu 
    1340                 1345                 1350             


Cys Ser  Ser Phe Ile Gly Pro  Thr Gly Ser Glu Arg  Lys Gly Leu 
    1355                 1360                 1365             


Phe Glu  Leu Thr Ser Arg Gly  Ser Ala Ala Asp Phe  Glu Phe Leu 
    1370                 1375                 1380             


Gly Val  Lys Ile Pro Arg Tyr  Arg Asp Tyr Thr Pro  Ser Ser Leu 
    1385                 1390                 1395             


Leu Lys  Asp Ala Thr Leu Ile  His Gln Ser Val Thr  Gly Leu Tyr 
    1400                 1405                 1410             


Glu Thr  Arg Ile Asp Leu Ala  Lys Leu Gly Glu Gly  Lys Arg Pro 
    1415                 1420                 1425             


Ala Ala  Thr Lys Lys Ala Gly  Gln Ala Lys Lys Lys  Lys 
    1430                 1435                 1440     


<210>  1366
<211>  1133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1366

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Trp Tyr Ala Ser Leu Met Ser Ala His His Leu Arg Val Gly Ile 
            20                  25                  30          


Asp Val Gly Thr His Ser Val Gly Leu Ala Thr Leu Arg Val Asp Asp 
        35                  40                  45              


His Gly Thr Pro Ile Glu Leu Leu Ser Ala Leu Ser His Ile His Asp 
    50                  55                  60                  


Ser Gly Val Gly Lys Glu Gly Lys Lys Asp His Asp Thr Arg Lys Lys 
65                  70                  75                  80  


Leu Ser Gly Ile Ala Arg Arg Ala Arg Arg Leu Leu His His Arg Arg 
                85                  90                  95      


Thr Gln Leu Gln Gln Leu Asp Glu Val Leu Arg Asp Leu Gly Phe Pro 
            100                 105                 110         


Ile Pro Thr Pro Gly Glu Phe Leu Asp Leu Asn Glu Gln Thr Asp Pro 
        115                 120                 125             


Tyr Arg Val Trp Arg Val Arg Ala Arg Leu Val Glu Glu Lys Leu Pro 
    130                 135                 140                 


Glu Glu Leu Arg Gly Pro Ala Ile Ser Met Ala Val Arg His Ile Ala 
145                 150                 155                 160 


Arg His Arg Gly Trp Arg Asn Pro Tyr Ser Lys Val Glu Ser Leu Leu 
                165                 170                 175     


Ser Pro Ala Glu Glu Ser Pro Phe Met Lys Ala Leu Arg Glu Arg Ile 
            180                 185                 190         


Leu Ala Thr Thr Gly Glu Val Leu Asp Asp Gly Ile Thr Pro Gly Gln 
        195                 200                 205             


Ala Met Ala Gln Val Ala Leu Thr His Asn Ile Ser Met Arg Gly Pro 
    210                 215                 220                 


Glu Gly Ile Leu Gly Lys Leu His Gln Ser Asp Asn Ala Asn Glu Ile 
225                 230                 235                 240 


Arg Lys Ile Cys Ala Arg Gln Gly Val Ser Pro Asp Val Cys Lys Gln 
                245                 250                 255     


Leu Leu Arg Ala Val Phe Lys Ala Asp Ser Pro Arg Gly Ser Ala Val 
            260                 265                 270         


Ser Arg Val Ala Pro Asp Pro Leu Pro Gly Gln Gly Ser Phe Arg Arg 
        275                 280                 285             


Ala Pro Lys Cys Asp Pro Glu Phe Gln Arg Phe Arg Ile Ile Ser Ile 
    290                 295                 300                 


Val Ala Asn Leu Arg Ile Ser Glu Thr Lys Gly Glu Asn Arg Pro Leu 
305                 310                 315                 320 


Thr Ala Asp Glu Arg Arg His Val Val Thr Phe Leu Thr Glu Asp Ser 
                325                 330                 335     


Gln Ala Asp Leu Thr Trp Val Asp Val Ala Glu Lys Leu Gly Val His 
            340                 345                 350         


Arg Arg Asp Leu Arg Gly Thr Ala Val His Thr Asp Asp Gly Glu Arg 
        355                 360                 365             


Ser Ala Ala Arg Pro Pro Ile Asp Ala Thr Asp Arg Ile Met Arg Gln 
    370                 375                 380                 


Thr Lys Ile Ser Ser Leu Lys Thr Trp Trp Glu Glu Ala Asp Ser Glu 
385                 390                 395                 400 


Gln Arg Gly Ala Met Ile Arg Tyr Leu Tyr Glu Asp Pro Thr Asp Ser 
                405                 410                 415     


Glu Cys Ala Glu Ile Ile Ala Glu Leu Pro Glu Glu Asp Gln Ala Lys 
            420                 425                 430         


Leu Asp Ser Leu His Leu Pro Ala Gly Arg Ala Ala Tyr Ser Arg Glu 
        435                 440                 445             


Ser Leu Thr Ala Leu Ser Asp His Met Leu Ala Thr Thr Asp Asp Leu 
    450                 455                 460                 


His Glu Ala Arg Lys Arg Leu Phe Gly Val Asp Asp Ser Trp Ala Pro 
465                 470                 475                 480 


Pro Ala Glu Ala Ile Asn Ala Pro Val Gly Asn Pro Ser Val Asp Arg 
                485                 490                 495     


Thr Leu Lys Ile Val Gly Arg Tyr Leu Ser Ala Val Glu Ser Met Trp 
            500                 505                 510         


Gly Thr Pro Glu Val Ile His Val Glu His Val Arg Asp Gly Phe Thr 
        515                 520                 525             


Ser Glu Arg Met Ala Asp Glu Arg Asp Lys Ala Asn Arg Arg Arg Tyr 
    530                 535                 540                 


Asn Asp Asn Gln Glu Ala Met Lys Lys Ile Gln Arg Asp Tyr Gly Lys 
545                 550                 555                 560 


Glu Gly Tyr Ile Ser Arg Gly Asp Ile Val Arg Leu Asp Ala Leu Glu 
                565                 570                 575     


Leu Gln Gly Cys Ala Cys Leu Tyr Cys Gly Thr Thr Ile Gly Tyr His 
            580                 585                 590         


Thr Cys Gln Leu Asp His Ile Val Pro Gln Ala Gly Pro Gly Ser Asn 
        595                 600                 605             


Asn Arg Arg Gly Asn Leu Val Ala Val Cys Glu Arg Cys Asn Arg Ser 
    610                 615                 620                 


Lys Ser Asn Thr Pro Phe Ala Val Trp Ala Gln Lys Cys Gly Ile Pro 
625                 630                 635                 640 


His Val Gly Val Lys Glu Ala Ile Gly Arg Val Arg Gly Trp Arg Lys 
                645                 650                 655     


Gln Thr Pro Asn Thr Ser Ser Glu Asp Leu Thr Arg Leu Lys Lys Glu 
            660                 665                 670         


Val Ile Ala Arg Leu Arg Arg Thr Gln Glu Asp Pro Glu Ile Asp Glu 
        675                 680                 685             


Arg Ser Met Glu Ser Val Ala Trp Met Ala Asn Glu Leu His His Arg 
    690                 695                 700                 


Ile Ala Ala Ala Tyr Pro Glu Thr Thr Val Met Val Tyr Arg Gly Ser 
705                 710                 715                 720 


Ile Thr Ala Ala Ala Arg Lys Ala Ala Gly Ile Asp Ser Arg Ile Asn 
                725                 730                 735     


Leu Ile Gly Glu Lys Gly Arg Lys Asp Arg Ile Asp Arg Arg His His 
            740                 745                 750         


Ala Val Asp Ala Ser Val Val Ala Leu Met Glu Ala Ser Val Ala Lys 
        755                 760                 765             


Thr Leu Ala Glu Arg Ser Ser Leu Arg Gly Glu Gln Arg Leu Thr Gly 
    770                 775                 780                 


Lys Glu Gln Thr Trp Lys Gln Tyr Thr Gly Ser Thr Val Gly Ala Arg 
785                 790                 795                 800 


Glu His Phe Glu Met Trp Arg Gly His Met Leu His Leu Thr Glu Leu 
                805                 810                 815     


Phe Asn Glu Arg Leu Ala Glu Asp Lys Val Tyr Val Thr Gln Asn Ile 
            820                 825                 830         


Arg Leu Arg Leu Ser Asp Gly Asn Ala His Thr Val Asn Pro Ser Lys 
        835                 840                 845             


Leu Val Ser His Arg Leu Gly Asp Gly Leu Thr Val Gln Gln Ile Asp 
    850                 855                 860                 


Arg Ala Cys Thr Pro Ala Leu Trp Cys Ala Leu Thr Arg Glu Lys Asp 
865                 870                 875                 880 


Phe Asp Glu Lys Asn Gly Leu Pro Ala Arg Glu Asp Arg Ala Ile Arg 
                885                 890                 895     


Val His Gly His Glu Ile Lys Ser Ser Asp Tyr Ile Gln Val Phe Ser 
            900                 905                 910         


Lys Arg Lys Lys Thr Asp Ser Asp Arg Asp Glu Thr Pro Phe Gly Ala 
        915                 920                 925             


Ile Ala Val Arg Gly Gly Phe Val Glu Ile Gly Pro Ser Ile His His 
    930                 935                 940                 


Ala Arg Ile Tyr Arg Val Glu Gly Lys Lys Pro Val Tyr Ala Met Leu 
945                 950                 955                 960 


Arg Val Phe Thr His Asp Leu Leu Ser Gln Arg His Gly Asp Leu Phe 
                965                 970                 975     


Ser Ala Val Ile Pro Pro Gln Ser Ile Ser Met Arg Cys Ala Glu Pro 
            980                 985                 990         


Lys Leu Arg Lys Ala Ile Thr Thr  Gly Asn Ala Thr Tyr  Leu Gly Trp 
        995                 1000                 1005             


Val Val  Val Gly Asp Glu Leu  Glu Ile Asn Val Asp  Ser Phe Thr 
    1010                 1015                 1020             


Lys Tyr  Ala Ile Gly Arg Phe  Leu Glu Asp Phe Pro  Asn Thr Thr 
    1025                 1030                 1035             


Arg Trp  Arg Ile Cys Gly Tyr  Asp Thr Asn Ser Lys  Leu Thr Leu 
    1040                 1045                 1050             


Lys Pro  Ile Val Leu Ala Ala  Glu Gly Leu Glu Asn  Pro Ser Ser 
    1055                 1060                 1065             


Ala Val  Asn Glu Ile Val Glu  Leu Lys Gly Trp Arg  Val Ala Ile 
    1070                 1075                 1080             


Asn Val  Leu Thr Lys Val His  Pro Thr Val Val Arg  Arg Asp Ala 
    1085                 1090                 1095             


Leu Gly  Arg Pro Arg Tyr Ser  Ser Arg Ser Asn Leu  Pro Thr Ser 
    1100                 1105                 1110             


Trp Thr  Ile Glu Lys Arg Pro  Ala Ala Thr Lys Lys  Ala Gly Gln 
    1115                 1120                 1125             


Ala Lys  Lys Lys Lys 
    1130             


<210>  1367
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1367

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
            20                  25                  30          


Gly Trp Ala Val Thr Thr Asp Asn Tyr Lys Val Pro Ser Lys Lys Met 
        35                  40                  45              


Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
    50                  55                  60                  


Gly Val Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
65                  70                  75                  80  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
                85                  90                  95      


Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
            100                 105                 110         


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
        115                 120                 125             


Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
    130                 135                 140                 


His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
145                 150                 155                 160 


Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
                165                 170                 175     


Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
            180                 185                 190         


Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
        195                 200                 205             


Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
    210                 215                 220                 


Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu Glu Lys Lys Asp Arg 
225                 230                 235                 240 


Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
                245                 250                 255     


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Arg Lys Cys Phe 
            260                 265                 270         


Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser Lys Glu Ser Tyr Asp 
        275                 280                 285             


Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
    290                 295                 300                 


Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Thr Val Thr Asp Asn Glu Thr Glu Ala Pro Leu Ser Ser Ala 
                325                 330                 335     


Met Ile Lys Arg Tyr Asn Glu His Lys Glu Asp Leu Ala Leu Leu Lys 
            340                 345                 350         


Glu Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr Asn Glu Val Phe Lys 
        355                 360                 365             


Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
    370                 375                 380                 


Gln Glu Asp Phe Tyr Val Tyr Leu Lys Lys Leu Leu Ala Glu Phe Glu 
385                 390                 395                 400 


Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
                405                 410                 415     


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
            420                 425                 430         


Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
        435                 440                 445             


Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
465                 470                 475                 480 


Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
                485                 490                 495     


Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
            500                 505                 510         


Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
        515                 520                 525             


Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
    530                 535                 540                 


Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
545                 550                 555                 560 


Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr 
                565                 570                 575     


Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Tyr Gly Tyr Asp Gly 
            580                 585                 590         


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
        595                 600                 605             


Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
    610                 615                 620                 


Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
625                 630                 635                 640 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
                645                 650                 655     


Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
            660                 665                 670         


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
        675                 680                 685             


Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser 
    690                 695                 700                 


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
705                 710                 715                 720 


Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Glu Asp Lys Gly Asn 
                725                 730                 735     


Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser Pro Ala Ile Lys Lys 
            740                 745                 750         


Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met 
        755                 760                 765             


Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn 
    770                 775                 780                 


Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg 
785                 790                 795                 800 


Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn 
                805                 810                 815     


Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp 
            820                 825                 830         


Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly 
        835                 840                 845             


Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile 
    850                 855                 860                 


Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile Asp Asn Lys Val Leu 
865                 870                 875                 880 


Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu 
                885                 890                 895     


Glu Val Val Lys Lys Arg Lys Thr Phe Trp Tyr Gln Leu Leu Lys Ser 
            900                 905                 910         


Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
        915                 920                 925             


Gly Gly Leu Ser Pro Glu Asp Lys Ala Gly Phe Ile Gln Arg Gln Leu 
    930                 935                 940                 


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu 
945                 950                 955                 960 


Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg Ala Val Arg Thr Val 
                965                 970                 975     


Lys Ile Ile Thr Leu Lys Ser Thr Leu Val Ser Gln Phe Arg Lys Asp 
            980                 985                 990         


Phe Glu Leu Tyr Lys Val Arg Glu  Ile Asn Asp Phe His  His Ala His 
        995                 1000                 1005             


Asp Ala  Tyr Leu Asn Ala Val  Val Ala Ser Ala Leu  Leu Lys Lys 
    1010                 1015                 1020             


Tyr Pro  Lys Leu Glu Pro Glu  Phe Val Tyr Gly Asp  Tyr Pro Lys 
    1025                 1030                 1035             


Tyr Asn  Ser Phe Arg Glu Arg  Lys Ser Ala Thr Glu  Lys Val Tyr 
    1040                 1045                 1050             


Phe Tyr  Ser Asn Ile Met Asn  Ile Phe Lys Lys Ser  Ile Ser Leu 
    1055                 1060                 1065             


Ala Asp  Gly Arg Val Ile Glu  Arg Pro Leu Ile Glu  Val Asn Glu 
    1070                 1075                 1080             


Glu Thr  Gly Glu Ser Val Trp  Asn Lys Glu Ser Asp  Leu Ala Thr 
    1085                 1090                 1095             


Val Arg  Arg Val Leu Ser Tyr  Pro Gln Val Asn Val  Val Lys Lys 
    1100                 1105                 1110             


Val Glu  Glu Gln Asn His Gly  Leu Asp Arg Gly Lys  Pro Lys Gly 
    1115                 1120                 1125             


Leu Phe  Asn Ala Asn Leu Ser  Ser Lys Pro Lys Pro  Asn Ser Asn 
    1130                 1135                 1140             


Glu Asn  Leu Val Gly Ala Lys  Glu Tyr Leu Asp Pro  Lys Lys Tyr 
    1145                 1150                 1155             


Gly Gly  Tyr Ala Gly Ile Ser  Asn Ser Phe Thr Val  Leu Val Lys 
    1160                 1165                 1170             


Gly Thr  Ile Glu Lys Gly Ala  Lys Lys Lys Ile Thr  Asn Val Leu 
    1175                 1180                 1185             


Glu Phe  Gln Gly Ile Ser Ile  Leu Asp Arg Ile Asn  Tyr Arg Lys 
    1190                 1195                 1200             


Asp Lys  Leu Asn Phe Leu Leu  Glu Lys Gly Tyr Lys  Asp Ile Glu 
    1205                 1210                 1215             


Leu Ile  Ile Glu Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Ser Asp 
    1220                 1225                 1230             


Gly Ser  Arg Arg Met Leu Ala  Ser Ile Leu Ser Thr  Asn Asn Lys 
    1235                 1240                 1245             


Arg Gly  Glu Ile His Lys Gly  Asn Gln Ile Phe Leu  Ser Gln Lys 
    1250                 1255                 1260             


Phe Val  Lys Leu Leu Tyr His  Ala Lys Arg Ile Ser  Asn Thr Ile 
    1265                 1270                 1275             


Asn Glu  Asn His Arg Lys Tyr  Val Glu Asn His Lys  Lys Glu Phe 
    1280                 1285                 1290             


Glu Glu  Leu Phe Tyr Tyr Ile  Leu Glu Phe Asn Glu  Asn Tyr Val 
    1295                 1300                 1305             


Gly Ala  Lys Lys Asn Gly Lys  Leu Leu Asn Ser Ala  Phe Gln Ser 
    1310                 1315                 1320             


Trp Gln  Asn His Ser Ile Asp  Glu Leu Cys Ser Ser  Phe Ile Gly 
    1325                 1330                 1335             


Pro Thr  Gly Ser Glu Arg Lys  Gly Leu Phe Glu Leu  Thr Ser Arg 
    1340                 1345                 1350             


Gly Ser  Ala Ala Asp Phe Glu  Phe Leu Gly Val Lys  Ile Pro Arg 
    1355                 1360                 1365             


Tyr Arg  Asp Tyr Thr Pro Ser  Ser Leu Leu Lys Asp  Ala Thr Leu 
    1370                 1375                 1380             


Ile His  Gln Ser Val Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1385                 1390                 1395             


Ala Lys  Leu Gly Glu Gly Lys  Arg Pro Ala Ala Thr  Lys Lys Ala 
    1400                 1405                 1410             


Gly Gln  Ala Lys Lys Lys Lys  
    1415                 1420 


<210>  1368
<211>  1114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1368

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ala Ala Phe Lys Pro Asn Ser Ile Asn Tyr Ile Leu Gly Leu Asp 
            20                  25                  30          


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu 
        35                  40                  45              


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
    50                  55                  60                  


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
65                  70                  75                  80  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
                85                  90                  95      


Arg Thr Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asn 
            100                 105                 110         


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
        115                 120                 125             


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
    130                 135                 140                 


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
145                 150                 155                 160 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
                165                 170                 175     


Gly Val Ala Gly Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
            180                 185                 190         


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
        195                 200                 205             


Arg Asn Gln Arg Ser Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
    210                 215                 220                 


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
225                 230                 235                 240 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
                245                 250                 255     


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
            260                 265                 270         


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
        275                 280                 285             


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
    290                 295                 300                 


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
305                 310                 315                 320 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
                325                 330                 335     


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
            340                 345                 350         


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
        355                 360                 365             


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
    370                 375                 380                 


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
385                 390                 395                 400 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
                405                 410                 415     


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
            420                 425                 430         


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
        435                 440                 445             


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
    450                 455                 460                 


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
465                 470                 475                 480 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
                485                 490                 495     


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
            500                 505                 510         


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
        515                 520                 525             


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
    530                 535                 540                 


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
545                 550                 555                 560 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
                565                 570                 575     


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
            580                 585                 590         


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
        595                 600                 605             


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
    610                 615                 620                 


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
625                 630                 635                 640 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
                645                 650                 655     


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
            660                 665                 670         


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
        675                 680                 685             


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
    690                 695                 700                 


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
705                 710                 715                 720 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
                725                 730                 735     


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
            740                 745                 750         


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
        755                 760                 765             


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
    770                 775                 780                 


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
785                 790                 795                 800 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
                805                 810                 815     


Asp Thr Leu Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
            820                 825                 830         


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
        835                 840                 845             


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
    850                 855                 860                 


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
865                 870                 875                 880 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
                885                 890                 895     


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
            900                 905                 910         


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
        915                 920                 925             


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
    930                 935                 940                 


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
945                 950                 955                 960 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
                965                 970                 975     


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
            980                 985                 990         


Arg Ala Val Val Gln Gly Lys Asp  Glu Glu Asp Trp Gln  Leu Ile Asp 
        995                 1000                 1005             


Asp Ser  Phe Asn Phe Lys Phe  Ser Leu His Pro Asn  Asp Leu Val 
    1010                 1015                 1020             


Glu Val  Ile Thr Lys Lys Ala  Arg Met Phe Gly Tyr  Phe Ala Ser 
    1025                 1030                 1035             


Cys His  Arg Gly Thr Gly Asn  Ile Asn Ile Arg Ile  His Asp Leu 
    1040                 1045                 1050             


Asp His  Lys Ile Gly Lys Asn  Gly Ile Leu Glu Gly  Ile Gly Val 
    1055                 1060                 1065             


Lys Thr  Ala Leu Ser Phe Gln  Lys Tyr Gln Ile Asp  Glu Leu Gly 
    1070                 1075                 1080             


Lys Glu  Ile Arg Pro Cys Arg  Leu Lys Lys Arg Pro  Pro Val Arg 
    1085                 1090                 1095             


Lys Arg  Pro Ala Ala Thr Lys  Lys Ala Gly Gln Ala  Lys Lys Lys 
    1100                 1105                 1110             


Lys 
    


<210>  1369
<211>  1366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1369

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
            20                  25                  30          


Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
        35                  40                  45              


Lys Ile Ala Gly Asp Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
    50                  55                  60                  


Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Ala Asp Arg Arg Met 
65                  70                  75                  80  


Ala Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
                85                  90                  95      


Tyr Leu Gln Gly Ile Phe Ala Glu Glu Met Ser Lys Thr Asp Ala Asn 
            100                 105                 110         


Phe Phe Cys Arg Leu Ser Asp Ser Phe Tyr Val Asp Asn Glu Lys Arg 
        115                 120                 125             


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Glu Tyr 
    130                 135                 140                 


His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
145                 150                 155                 160 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
                165                 170                 175     


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
            180                 185                 190         


Gln Asn Thr Ser Val Asp Gly Ile Tyr Lys Gln Phe Ile Gln Thr Tyr 
        195                 200                 205             


Asn Gln Val Phe Ala Ser Gly Ile Glu Asp Gly Ser Leu Lys Lys Leu 
    210                 215                 220                 


Glu Asp Asn Lys Asp Val Ala Lys Ile Leu Val Glu Lys Val Thr Arg 
225                 230                 235                 240 


Lys Glu Lys Leu Glu Arg Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser 
                245                 250                 255     


Ala Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Ser Lys Gly 
            260                 265                 270         


Asn Phe Gln Lys Pro Phe Asp Leu Ile Glu Lys Ser Asp Ile Glu Cys 
        275                 280                 285             


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ser Leu Leu Ala Leu Ile 
    290                 295                 300                 


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Ala Tyr Ser 
305                 310                 315                 320 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Ala Glu Thr Glu Thr Asn 
                325                 330                 335     


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Thr His Glu Glu 
            340                 345                 350         


Asp Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys His 
        355                 360                 365             


Tyr Glu Glu Ile Phe Ser Asn Thr Glu Lys His Gly Tyr Ala Gly Tyr 
    370                 375                 380                 


Ile Asp Gly Lys Thr Lys Gln Ala Asp Phe Tyr Lys Tyr Met Lys Met 
385                 390                 395                 400 


Thr Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu 
                405                 410                 415     


Lys Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile 
            420                 425                 430         


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
        435                 440                 445             


Ala Lys Tyr Tyr Pro Phe Leu Lys Glu Asn Tyr Asp Lys Ile Lys Ser 
    450                 455                 460                 


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly 
465                 470                 475                 480 


Gln Ser Glu Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
                485                 490                 495     


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
            500                 505                 510         


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
        515                 520                 525             


Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Leu Val Tyr Asn 
    530                 535                 540                 


Glu Leu Thr Lys Val Arg Tyr Ile Asn Asp Gln Gly Lys Thr Ser Tyr 
545                 550                 555                 560 


Phe Ser Gly Gln Glu Lys Glu Gln Ile Phe Asn Asp Leu Phe Lys Gln 
                565                 570                 575     


Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Arg Asn Met 
            580                 585                 590         


Ser His Val Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
        595                 600                 605             


Ser Ser Tyr Ser Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln 
    610                 615                 620                 


Glu Ile Leu Asp Asn Pro Val Asn Thr Glu Met Leu Glu Asn Ile Val 
625                 630                 635                 640 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
                645                 650                 655     


Gln Gln Phe Ser Asp Val Leu Asp Gly Val Val Leu Lys Lys Leu Glu 
            660                 665                 670         


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Met 
        675                 680                 685             


Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
    690                 695                 700                 


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
705                 710                 715                 720 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Thr Thr Ala 
                725                 730                 735     


Asp Lys Asp Ile Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
            740                 745                 750         


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
        755                 760                 765             


Ser Val Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
    770                 775                 780                 


Glu Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr 
785                 790                 795                 800 


Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
                805                 810                 815     


Glu His Pro Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu 
            820                 825                 830         


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp 
        835                 840                 845             


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
    850                 855                 860                 


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
865                 870                 875                 880 


Gly Asn Arg Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg 
                885                 890                 895     


Lys Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser 
            900                 905                 910         


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
        915                 920                 925             


Glu Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg 
    930                 935                 940                 


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Tyr 
945                 950                 955                 960 


Glu Lys Asp Asp His Gly Asn Thr Met Lys Gln Val Arg Ile Val Thr 
                965                 970                 975     


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
            980                 985                 990         


Lys Val Arg Asp Val Asn Asp Tyr  His His Ala His Asp  Ala Tyr Leu 
        995                 1000                 1005             


Asn Gly  Val Val Ala Asn Thr  Leu Leu Lys Val Tyr  Pro Gln Leu 
    1010                 1015                 1020             


Glu Pro  Glu Phe Val Tyr Gly  Asp Tyr His Gln Phe  Asp Trp Phe 
    1025                 1030                 1035             


Lys Ala  Asn Lys Ala Thr Ala  Lys Lys Gln Phe Tyr  Thr Asn Ile 
    1040                 1045                 1050             


Met Leu  Phe Phe Ala Gln Lys  Asp Arg Ile Ile Asp  Glu Asn Gly 
    1055                 1060                 1065             


Glu Ile  Leu Trp Asp Lys Lys  Tyr Leu Asp Thr Val  Lys Lys Val 
    1070                 1075                 1080             


Met Ser  Tyr Arg Gln Met Asn  Ile Val Lys Lys Thr  Glu Ile Gln 
    1085                 1090                 1095             


Lys Gly  Glu Phe Ser Lys Ala  Thr Ile Lys Pro Lys  Gly Asn Ser 
    1100                 1105                 1110             


Ser Lys  Leu Ile Pro Arg Lys  Thr Asn Trp Asp Pro  Met Lys Tyr 
    1115                 1120                 1125             


Gly Gly  Leu Asp Ser Pro Asn  Met Ala Tyr Ala Val  Val Ile Glu 
    1130                 1135                 1140             


Tyr Ala  Lys Gly Lys Asn Lys  Leu Val Phe Glu Lys  Lys Ile Ile 
    1145                 1150                 1155             


Arg Val  Thr Ile Met Glu Arg  Lys Ala Phe Glu Lys  Asp Glu Lys 
    1160                 1165                 1170             


Ala Phe  Leu Glu Glu Gln Gly  Tyr Arg Gln Pro Lys  Val Leu Ala 
    1175                 1180                 1185             


Lys Leu  Pro Lys Tyr Thr Leu  Tyr Glu Cys Glu Glu  Gly Arg Arg 
    1190                 1195                 1200             


Arg Met  Leu Ala Ser Ala Asn  Glu Ala Gln Lys Gly  Asn Gln Gln 
    1205                 1210                 1215             


Val Leu  Pro Asn His Leu Val  Thr Leu Leu His His  Ala Ala Asn 
    1220                 1225                 1230             


Cys Glu  Val Ser Asp Gly Lys  Ser Leu Asp Tyr Ile  Glu Ser Asn 
    1235                 1240                 1245             


Arg Glu  Met Phe Ala Glu Leu  Leu Ala His Val Ser  Glu Phe Ala 
    1250                 1255                 1260             


Lys Arg  Tyr Thr Leu Ala Glu  Ala Asn Leu Asn Lys  Ile Asn Gln 
    1265                 1270                 1275             


Leu Phe  Glu Gln Asn Lys Glu  Gly Asp Ile Lys Ala  Ile Ala Gln 
    1280                 1285                 1290             


Ser Phe  Val Asp Leu Met Ala  Phe Asn Ala Met Gly  Ala Pro Ala 
    1295                 1300                 1305             


Ser Phe  Lys Phe Phe Glu Thr  Thr Ile Glu Arg Lys  Arg Tyr Asn 
    1310                 1315                 1320             


Asn Leu  Lys Glu Leu Leu Asn  Ser Thr Ile Ile Tyr  Gln Ser Ile 
    1325                 1330                 1335             


Thr Gly  Leu Tyr Glu Ser Arg  Lys Arg Leu Asp Asp  Lys Arg Pro 
    1340                 1345                 1350             


Ala Ala  Thr Lys Lys Ala Gly  Gln Ala Lys Lys Lys  Lys 
    1355                 1360                 1365     


<210>  1370
<211>  1377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1370

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
            20                  25                  30          


Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
        35                  40                  45              


Lys Val Leu Gly Asn Thr Asp Lys Ser His Ile Glu Lys Asn Leu Leu 
    50                  55                  60                  


Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
65                  70                  75                  80  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
                85                  90                  95      


Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Gly Lys Val Asp Asp Ser 
            100                 105                 110         


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Thr Glu Asp Lys Arg 
        115                 120                 125             


Gly Glu Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Lys Tyr 
    130                 135                 140                 


His Glu Asn Phe Pro Thr Ile Tyr His Leu Arg Gln Tyr Leu Ala Asp 
145                 150                 155                 160 


Asn Pro Glu Lys Val Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
                165                 170                 175     


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Lys Phe Asp Thr 
            180                 185                 190         


Arg Asn Asn Asp Val Gln Arg Leu Phe Gln Glu Phe Leu Ala Val Tyr 
        195                 200                 205             


Asp Asn Thr Phe Glu Asn Ser Ser Leu Gln Glu Gln Asn Val Gln Val 
    210                 215                 220                 


Glu Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
225                 230                 235                 240 


Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Arg Phe Ala Glu 
                245                 250                 255     


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
            260                 265                 270         


Glu Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
        275                 280                 285             


Glu Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu 
    290                 295                 300                 


Leu Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
305                 310                 315                 320 


Ile Leu Thr Val Thr Asp Val Gly Thr Lys Ala Pro Leu Ser Ala Ser 
                325                 330                 335     


Met Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys 
            340                 345                 350         


Gln Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser 
        355                 360                 365             


Asp Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
    370                 375                 380                 


Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu 
385                 390                 395                 400 


Gly Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
                405                 410                 415     


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
            420                 425                 430         


Gln Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe 
        435                 440                 445             


Leu Ala Asp Asn Gln Asp Arg Ile Glu Lys Leu Leu Thr Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp 
465                 470                 475                 480 


Leu Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu 
                485                 490                 495     


Ile Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
            500                 505                 510         


Asn Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser 
        515                 520                 525             


Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
    530                 535                 540                 


Tyr Lys Thr Glu Gln Gly Lys Thr Ala Phe Phe Asp Ala Asn Met Lys 
545                 550                 555                 560 


Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr Lys 
                565                 570                 575     


Asp Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
            580                 585                 590         


Val Asp Leu Thr Gly Leu Asp Lys Glu Asn Lys Val Phe Asn Ala Ser 
        595                 600                 605             


Tyr Gly Thr Tyr His Asp Leu Cys Lys Ile Leu Asp Lys Asp Phe Leu 
    610                 615                 620                 


Asp Asn Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu 
625                 630                 635                 640 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Asn Tyr 
                645                 650                 655     


Ser Asp Leu Leu Thr Lys Glu Gln Val Lys Lys Leu Glu Arg Arg His 
            660                 665                 670         


Tyr Thr Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg 
        675                 680                 685             


Asn Lys Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
    690                 695                 700                 


Asn Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser 
705                 710                 715                 720 


Phe Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn 
                725                 730                 735     


Leu Asn Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys 
            740                 745                 750         


Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met 
        755                 760                 765             


Gly His Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
    770                 775                 780                 


Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu 
785                 790                 795                 800 


Thr Asp Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
                805                 810                 815     


Val Glu Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
            820                 825                 830         


Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr 
        835                 840                 845             


Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
    850                 855                 860                 


Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg 
865                 870                 875                 880 


Gly Lys Ser Asp Asp Val Pro Ser Lys Asp Val Val Arg Lys Met Lys 
                885                 890                 895     


Ser Tyr Trp Ser Lys Leu Leu Ser Ala Lys Leu Ile Thr Gln Arg Lys 
            900                 905                 910         


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp 
        915                 920                 925             


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
    930                 935                 940                 


Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Thr Asp 
945                 950                 955                 960 


Glu Asn Asn Lys Lys Ile Arg Gln Val Lys Ile Val Thr Leu Lys Ser 
                965                 970                 975     


Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
            980                 985                 990         


Glu Ile Asn Asp Tyr His His Ala  His Asp Ala Tyr Leu  Asn Ala Val 
        995                 1000                 1005             


Ile Gly  Lys Ala Leu Leu Gly  Val Tyr Pro Gln Leu  Glu Pro Glu 
    1010                 1015                 1020             


Phe Val  Tyr Gly Asp Tyr Pro  His Phe His Gly His  Lys Glu Asn 
    1025                 1030                 1035             


Lys Ala  Thr Ala Lys Lys Phe  Phe Tyr Ser Asn Ile  Met Asn Phe 
    1040                 1045                 1050             


Phe Lys  Lys Asp Asp Val Arg  Thr Asp Lys Asn Gly  Glu Ile Ile 
    1055                 1060                 1065             


Trp Lys  Lys Asp Glu His Ile  Ser Asn Ile Lys Lys  Val Leu Ser 
    1070                 1075                 1080             


Tyr Pro  Gln Val Asn Ile Val  Lys Lys Val Glu Glu  Gln Thr Gly 
    1085                 1090                 1095             


Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys Gly Asn  Ser Asp Lys 
    1100                 1105                 1110             


Leu Ile  Pro Arg Lys Thr Lys  Lys Phe Tyr Trp Asp  Thr Lys Lys 
    1115                 1120                 1125             


Tyr Gly  Gly Phe Asp Ser Pro  Ile Val Ala Tyr Ser  Ile Leu Val 
    1130                 1135                 1140             


Ile Ala  Asp Ile Glu Lys Gly  Lys Ser Lys Lys Leu  Lys Thr Val 
    1145                 1150                 1155             


Lys Ala  Leu Val Gly Val Thr  Ile Met Glu Lys Met  Thr Phe Glu 
    1160                 1165                 1170             


Arg Asp  Pro Val Ala Phe Leu  Glu Arg Lys Gly Tyr  Arg Asn Val 
    1175                 1180                 1185             


Gln Glu  Glu Asn Ile Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Lys 
    1190                 1195                 1200             


Leu Glu  Asn Gly Arg Lys Arg  Leu Leu Ala Ser Ala  Arg Glu Leu 
    1205                 1210                 1215             


Gln Lys  Gly Asn Glu Ile Val  Leu Pro Asn His Leu  Gly Thr Leu 
    1220                 1225                 1230             


Leu Tyr  His Ala Lys Asn Ile  His Lys Val Asp Glu  Pro Lys His 
    1235                 1240                 1245             


Leu Asp  Tyr Val Asp Lys His  Lys Asp Glu Phe Lys  Glu Leu Leu 
    1250                 1255                 1260             


Asp Val  Val Ser Asn Phe Ser  Lys Lys Tyr Thr Leu  Ala Glu Gly 
    1265                 1270                 1275             


Asn Leu  Glu Lys Ile Lys Glu  Leu Tyr Ala Gln Asn  Asn Gly Glu 
    1280                 1285                 1290             


Asp Leu  Lys Glu Leu Ala Ser  Ser Phe Ile Asn Leu  Leu Thr Phe 
    1295                 1300                 1305             


Thr Ala  Ile Gly Ala Pro Ala  Thr Phe Lys Phe Phe  Asp Lys Asn 
    1310                 1315                 1320             


Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Thr Glu Ile  Leu Asn Ala 
    1325                 1330                 1335             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1340                 1345                 1350             


Asp Leu  Asn Lys Leu Gly Gly  Asp Lys Arg Pro Ala  Ala Thr Lys 
    1355                 1360                 1365             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1370                 1375         


<210>  1371
<211>  1088
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1371

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Gln Thr Thr Asn Leu Ser Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
            20                  25                  30          


Ala Ser Val Gly Trp Ala Val Val Glu Ile Asn Glu Asn Glu Asp Pro 
        35                  40                  45              


Ile Gly Leu Ile Asp Val Gly Val Arg Ile Phe Glu Arg Ala Glu Val 
    50                  55                  60                  


Pro Lys Thr Gly Glu Ser Leu Ala Leu Ser Arg Arg Leu Ala Arg Ser 
65                  70                  75                  80  


Thr Arg Arg Leu Ile Arg Arg Arg Ala His Arg Leu Leu Leu Ala Lys 
                85                  90                  95      


Arg Phe Leu Lys Arg Glu Gly Ile Leu Ser Thr Ile Asp Leu Glu Lys 
            100                 105                 110         


Gly Leu Pro Asn Gln Ala Trp Glu Leu Arg Val Ala Gly Leu Glu Arg 
        115                 120                 125             


Arg Leu Ser Ala Ile Glu Trp Gly Ala Val Leu Leu His Leu Ile Lys 
    130                 135                 140                 


His Arg Gly Tyr Leu Ser Lys Arg Lys Asn Glu Ser Gln Thr Asn Asn 
145                 150                 155                 160 


Lys Glu Leu Gly Ala Leu Leu Ser Gly Val Ala Gln Asn His Gln Leu 
                165                 170                 175     


Leu Gln Ser Asp Asp Tyr Arg Thr Pro Ala Glu Leu Ala Leu Lys Lys 
            180                 185                 190         


Phe Ala Lys Glu Glu Gly His Ile Arg Asn Gln Arg Gly Ala Tyr Thr 
        195                 200                 205             


His Thr Phe Asn Arg Leu Asp Leu Leu Ala Glu Leu Asn Leu Leu Phe 
    210                 215                 220                 


Ala Gln Gln His Gln Phe Gly Asn Pro His Cys Lys Glu His Ile Gln 
225                 230                 235                 240 


Gln Tyr Met Thr Glu Leu Leu Met Trp Gln Lys Pro Ala Leu Ser Gly 
                245                 250                 255     


Glu Ala Ile Leu Lys Met Leu Gly Lys Cys Thr His Glu Lys Asn Glu 
            260                 265                 270         


Phe Lys Ala Ala Lys His Thr Tyr Ser Ala Glu Arg Phe Val Trp Leu 
        275                 280                 285             


Thr Lys Leu Asn Asn Leu Arg Ile Leu Glu Asp Gly Ala Glu Arg Ala 
    290                 295                 300                 


Leu Asn Glu Glu Glu Arg Gln Leu Leu Ile Asn His Pro Tyr Glu Lys 
305                 310                 315                 320 


Ser Lys Leu Thr Tyr Ala Gln Val Arg Lys Leu Leu Gly Leu Ser Glu 
                325                 330                 335     


Gln Ala Ile Phe Lys His Leu Arg Tyr Ser Lys Glu Asn Ala Glu Ser 
            340                 345                 350         


Ala Thr Phe Met Glu Leu Lys Ala Trp His Ala Ile Arg Lys Ala Leu 
        355                 360                 365             


Glu Asn Gln Gly Leu Lys Asp Thr Trp Gln Asp Leu Ala Lys Lys Pro 
    370                 375                 380                 


Asp Leu Leu Asp Glu Ile Gly Thr Ala Phe Ser Leu Tyr Lys Thr Asp 
385                 390                 395                 400 


Glu Asp Ile Gln Gln Tyr Leu Thr Asn Lys Val Pro Asn Ser Val Ile 
                405                 410                 415     


Asn Ala Leu Leu Val Ser Leu Asn Phe Asp Lys Phe Ile Glu Leu Ser 
            420                 425                 430         


Leu Lys Ser Leu Arg Lys Ile Leu Pro Leu Met Glu Gln Gly Lys Arg 
        435                 440                 445             


Tyr Asp Gln Ala Cys Arg Glu Ile Tyr Gly His His Tyr Gly Glu Ala 
    450                 455                 460                 


Asn Gln Lys Thr Ser Gln Leu Leu Pro Ala Ile Pro Ala Gln Glu Ile 
465                 470                 475                 480 


Arg Asn Pro Val Val Leu Arg Thr Leu Ser Gln Ala Arg Lys Val Ile 
                485                 490                 495     


Asn Ala Ile Ile Arg Gln Tyr Gly Ser Pro Ala Arg Val His Ile Glu 
            500                 505                 510         


Thr Gly Arg Glu Leu Gly Lys Ser Phe Lys Glu Arg Arg Glu Ile Gln 
        515                 520                 525             


Lys Gln Gln Glu Asp Asn Arg Thr Lys Arg Glu Ser Ala Val Gln Lys 
    530                 535                 540                 


Phe Lys Glu Leu Phe Ser Asp Phe Ser Ser Glu Pro Lys Ser Lys Asp 
545                 550                 555                 560 


Ile Leu Lys Phe Arg Leu Tyr Glu Gln Gln His Gly Lys Cys Leu Tyr 
                565                 570                 575     


Ser Gly Lys Glu Ile Asn Ile His Arg Leu Asn Glu Lys Gly Tyr Val 
            580                 585                 590         


Glu Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe 
        595                 600                 605             


Asn Asn Lys Val Leu Val Leu Ala Ser Glu Asn Gln Asn Lys Gly Asn 
    610                 615                 620                 


Gln Thr Pro Tyr Glu Trp Leu Gln Gly Lys Ile Asn Ser Glu Arg Trp 
625                 630                 635                 640 


Lys Asn Phe Val Ala Leu Val Leu Gly Ser Gln Cys Ser Ala Ala Lys 
                645                 650                 655     


Lys Gln Arg Leu Leu Thr Gln Val Ile Asp Asp Asn Lys Phe Ile Asp 
            660                 665                 670         


Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ala Arg Phe Leu Ser Asn Tyr 
        675                 680                 685             


Ile Gln Glu Asn Leu Leu Leu Val Gly Lys Asn Lys Lys Asn Val Phe 
    690                 695                 700                 


Thr Pro Asn Gly Gln Ile Thr Ala Leu Leu Arg Ser Arg Trp Gly Leu 
705                 710                 715                 720 


Ile Lys Ala Arg Glu Asn Asn Asn Arg His His Ala Leu Asp Ala Ile 
                725                 730                 735     


Val Val Ala Cys Ala Thr Pro Ser Met Gln Gln Lys Ile Thr Arg Phe 
            740                 745                 750         


Ile Arg Phe Lys Glu Val His Pro Tyr Lys Ile Glu Asn Arg Tyr Glu 
        755                 760                 765             


Met Val Asp Gln Glu Ser Gly Glu Ile Ile Ser Pro His Phe Pro Glu 
    770                 775                 780                 


Pro Trp Ala Tyr Phe Arg Gln Glu Val Asn Ile Arg Val Phe Asp Asn 
785                 790                 795                 800 


His Pro Asp Thr Val Leu Lys Glu Met Leu Pro Asp Arg Pro Gln Ala 
                805                 810                 815     


Asn His Gln Phe Val Gln Pro Leu Phe Val Ser Arg Ala Pro Thr Arg 
            820                 825                 830         


Lys Met Ser Gly Gln Gly His Met Glu Thr Ile Lys Ser Ala Lys Arg 
        835                 840                 845             


Leu Ala Glu Gly Ile Ser Val Leu Arg Ile Pro Leu Thr Gln Leu Lys 
    850                 855                 860                 


Pro Asn Leu Leu Glu Asn Met Val Asn Lys Glu Arg Glu Pro Ala Leu 
865                 870                 875                 880 


Tyr Ala Gly Leu Lys Ala Arg Leu Ala Glu Phe Asn Gln Asp Pro Ala 
                885                 890                 895     


Lys Ala Phe Ala Thr Pro Phe Tyr Lys Gln Gly Gly Gln Gln Val Lys 
            900                 905                 910         


Ala Ile Arg Val Glu Gln Val Gln Lys Ser Gly Val Leu Val Arg Glu 
        915                 920                 925             


Asn Asn Gly Val Ala Asp Asn Ala Ser Ile Val Arg Thr Asp Val Phe 
    930                 935                 940                 


Ile Lys Asn Asn Lys Phe Phe Leu Val Pro Ile Tyr Thr Trp Gln Val 
945                 950                 955                 960 


Ala Lys Gly Ile Leu Pro Asn Lys Ala Ile Val Ala His Lys Asn Glu 
                965                 970                 975     


Asp Glu Trp Glu Glu Met Asp Glu Gly Ala Lys Phe Lys Phe Ser Leu 
            980                 985                 990         


Phe Pro Asn Asp Leu Val Glu Leu  Lys Thr Lys Lys Glu  Tyr Phe Phe 
        995                 1000                 1005             


Gly Tyr  Tyr Ile Gly Leu Asp  Arg Ala Thr Gly Asn  Ile Ser Leu 
    1010                 1015                 1020             


Lys Glu  His Asp Gly Glu Ile  Ser Lys Gly Lys Asp  Gly Val Tyr 
    1025                 1030                 1035             


Arg Val  Gly Val Lys Leu Ala  Leu Ser Phe Glu Lys  Tyr Gln Val 
    1040                 1045                 1050             


Asp Glu  Leu Gly Lys Asn Arg  Gln Ile Cys Arg Pro  Gln Gln Arg 
    1055                 1060                 1065             


Gln Pro  Val Arg Lys Arg Pro  Ala Ala Thr Lys Lys  Ala Gly Gln 
    1070                 1075                 1080             


Ala Lys  Lys Lys Lys 
    1085             


<210>  1372
<211>  1114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1372

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
            20                  25                  30          


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
        35                  40                  45              


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
    50                  55                  60                  


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
65                  70                  75                  80  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
                85                  90                  95      


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
            100                 105                 110         


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
        115                 120                 125             


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
    130                 135                 140                 


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
145                 150                 155                 160 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
                165                 170                 175     


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
            180                 185                 190         


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
        195                 200                 205             


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
    210                 215                 220                 


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
225                 230                 235                 240 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
                245                 250                 255     


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
            260                 265                 270         


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
        275                 280                 285             


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
    290                 295                 300                 


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
305                 310                 315                 320 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
                325                 330                 335     


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
            340                 345                 350         


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
        355                 360                 365             


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
    370                 375                 380                 


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
385                 390                 395                 400 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
                405                 410                 415     


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
            420                 425                 430         


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
        435                 440                 445             


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
    450                 455                 460                 


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
465                 470                 475                 480 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
                485                 490                 495     


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
            500                 505                 510         


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
        515                 520                 525             


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
    530                 535                 540                 


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
545                 550                 555                 560 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
                565                 570                 575     


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
            580                 585                 590         


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
        595                 600                 605             


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
    610                 615                 620                 


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
625                 630                 635                 640 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
                645                 650                 655     


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
            660                 665                 670         


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
        675                 680                 685             


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
    690                 695                 700                 


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
705                 710                 715                 720 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
                725                 730                 735     


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
            740                 745                 750         


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
        755                 760                 765             


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
    770                 775                 780                 


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
785                 790                 795                 800 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
                805                 810                 815     


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
            820                 825                 830         


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
        835                 840                 845             


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
    850                 855                 860                 


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
865                 870                 875                 880 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
                885                 890                 895     


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
            900                 905                 910         


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
        915                 920                 925             


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
    930                 935                 940                 


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
945                 950                 955                 960 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
                965                 970                 975     


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
            980                 985                 990         


Arg Ala Val Val Gln Gly Lys Asp  Glu Glu Asp Trp Gln  Leu Ile Asp 
        995                 1000                 1005             


Asp Ser  Phe Asn Phe Lys Phe  Ser Leu His Pro Asn  Asp Leu Val 
    1010                 1015                 1020             


Glu Val  Ile Thr Lys Lys Ala  Arg Met Phe Gly Tyr  Phe Ala Ser 
    1025                 1030                 1035             


Cys His  Arg Gly Thr Gly Asn  Ile Asn Ile Arg Ile  His Asp Leu 
    1040                 1045                 1050             


Asp His  Lys Ile Gly Lys Asn  Gly Ile Leu Glu Gly  Ile Gly Val 
    1055                 1060                 1065             


Lys Thr  Ala Leu Ser Phe Gln  Lys Tyr Gln Ile Asp  Glu Leu Gly 
    1070                 1075                 1080             


Lys Glu  Ile Arg Pro Cys Arg  Leu Lys Lys Arg Pro  Pro Val Arg 
    1085                 1090                 1095             


Lys Arg  Pro Ala Ala Thr Lys  Lys Ala Gly Gln Ala  Lys Lys Lys 
    1100                 1105                 1110             


Lys 
    


<210>  1373
<211>  1116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1373

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Lys Tyr His Val Gly Ile Asp Val Gly Thr Phe Ser Val Gly Leu 
            20                  25                  30          


Ala Ala Ile Glu Val Asp Asp Ala Gly Met Pro Ile Lys Thr Leu Ser 
        35                  40                  45              


Leu Val Ser His Ile His Asp Ser Gly Leu Asp Pro Asp Glu Ile Lys 
    50                  55                  60                  


Ser Ala Val Thr Arg Leu Ala Ser Ser Gly Ile Ala Arg Arg Thr Arg 
65                  70                  75                  80  


Arg Leu Tyr Arg Arg Lys Arg Arg Arg Leu Gln Gln Leu Asp Lys Phe 
                85                  90                  95      


Ile Gln Arg Gln Gly Trp Pro Val Ile Glu Leu Glu Asp Tyr Ser Asp 
            100                 105                 110         


Pro Leu Tyr Pro Trp Lys Val Arg Ala Glu Leu Ala Ala Ser Tyr Ile 
        115                 120                 125             


Ala Asp Glu Lys Glu Arg Gly Glu Lys Leu Ser Val Ala Leu Arg His 
    130                 135                 140                 


Ile Ala Arg His Arg Gly Trp Arg Asn Pro Tyr Ala Lys Val Ser Ser 
145                 150                 155                 160 


Leu Tyr Leu Pro Asp Gly Pro Ser Asp Ala Phe Lys Ala Ile Arg Glu 
                165                 170                 175     


Glu Ile Lys Arg Ala Ser Gly Gln Pro Val Pro Glu Thr Ala Thr Val 
            180                 185                 190         


Gly Gln Met Val Thr Leu Cys Glu Leu Gly Thr Leu Lys Leu Arg Gly 
        195                 200                 205             


Glu Gly Gly Val Leu Ser Ala Arg Leu Gln Gln Ser Asp Tyr Ala Arg 
    210                 215                 220                 


Glu Ile Gln Glu Ile Cys Arg Met Gln Glu Ile Gly Gln Glu Leu Tyr 
225                 230                 235                 240 


Arg Lys Ile Ile Asp Val Val Phe Ala Ala Glu Ser Pro Lys Gly Ser 
                245                 250                 255     


Ala Ser Ser Arg Val Gly Lys Asp Pro Leu Gln Pro Gly Lys Asn Arg 
            260                 265                 270         


Ala Leu Lys Ala Ser Asp Ala Phe Gln Arg Tyr Arg Ile Ala Ala Leu 
        275                 280                 285             


Ile Gly Asn Leu Arg Val Arg Val Asp Gly Glu Lys Arg Ile Leu Ser 
    290                 295                 300                 


Val Glu Glu Lys Asn Leu Val Phe Asp His Leu Val Asn Leu Thr Pro 
305                 310                 315                 320 


Lys Lys Glu Pro Glu Trp Val Thr Ile Ala Glu Ile Leu Gly Ile Asp 
                325                 330                 335     


Arg Gly Gln Leu Ile Gly Thr Ala Thr Met Thr Asp Asp Gly Glu Arg 
            340                 345                 350         


Ala Gly Ala Arg Pro Pro Thr His Asp Thr Asn Arg Ser Ile Val Asn 
        355                 360                 365             


Ser Arg Ile Ala Pro Leu Val Asp Trp Trp Lys Thr Ala Ser Ala Leu 
    370                 375                 380                 


Glu Gln His Ala Met Val Lys Ala Leu Ser Asn Ala Glu Val Asp Asp 
385                 390                 395                 400 


Phe Asp Ser Pro Glu Gly Ala Lys Val Gln Ala Phe Phe Ala Asp Leu 
                405                 410                 415     


Asp Asp Asp Val His Ala Lys Leu Asp Ser Leu His Leu Pro Val Gly 
            420                 425                 430         


Arg Ala Ala Tyr Ser Glu Asp Thr Leu Val Arg Leu Thr Arg Arg Met 
        435                 440                 445             


Leu Ser Asp Gly Val Asp Leu Tyr Thr Ala Arg Leu Gln Glu Phe Gly 
    450                 455                 460                 


Ile Glu Pro Ser Trp Thr Pro Pro Thr Pro Arg Ile Gly Glu Pro Val 
465                 470                 475                 480 


Gly Asn Pro Ala Val Asp Arg Val Leu Lys Thr Val Ser Arg Trp Leu 
                485                 490                 495     


Glu Ser Ala Thr Lys Thr Trp Gly Ala Pro Glu Arg Val Ile Ile Glu 
            500                 505                 510         


His Val Arg Glu Gly Phe Val Thr Glu Lys Arg Ala Arg Glu Met Asp 
        515                 520                 525             


Gly Asp Met Arg Arg Arg Ala Ala Arg Asn Ala Lys Leu Phe Gln Glu 
    530                 535                 540                 


Met Gln Glu Lys Leu Asn Val Gln Gly Lys Pro Ser Arg Ala Asp Leu 
545                 550                 555                 560 


Trp Arg Tyr Gln Ser Val Gln Arg Gln Asn Cys Gln Cys Ala Tyr Cys 
                565                 570                 575     


Gly Ser Pro Ile Thr Phe Ser Asn Ser Glu Met Asp His Ile Val Pro 
            580                 585                 590         


Arg Ala Gly Gln Gly Ser Thr Asn Thr Arg Glu Asn Leu Val Ala Val 
        595                 600                 605             


Cys His Arg Cys Asn Gln Ser Lys Gly Asn Thr Pro Phe Ala Ile Trp 
    610                 615                 620                 


Ala Lys Asn Thr Ser Ile Glu Gly Val Ser Val Lys Glu Ala Val Glu 
625                 630                 635                 640 


Arg Thr Arg His Trp Val Thr Asp Thr Gly Met Arg Ser Thr Asp Phe 
                645                 650                 655     


Lys Lys Phe Thr Lys Ala Val Val Glu Arg Phe Gln Arg Ala Thr Met 
            660                 665                 670         


Asp Glu Glu Ile Asp Ala Arg Ser Met Glu Ser Val Ala Trp Met Ala 
        675                 680                 685             


Asn Glu Leu Arg Ser Arg Val Ala Gln His Phe Ala Ser His Gly Thr 
    690                 695                 700                 


Thr Val Arg Val Tyr Arg Gly Ser Leu Thr Ala Glu Ala Arg Arg Ala 
705                 710                 715                 720 


Ser Gly Ile Ser Gly Lys Leu Lys Phe Phe Asp Gly Val Gly Lys Ser 
                725                 730                 735     


Arg Leu Asp Arg Arg His His Ala Ile Asp Ala Ala Val Ile Ala Phe 
            740                 745                 750         


Thr Ser Asp Tyr Val Ala Glu Thr Leu Ala Val Arg Ser Asn Leu Lys 
        755                 760                 765             


Gln Ser Gln Ala His Arg Gln Glu Ala Pro Gln Trp Arg Glu Phe Thr 
    770                 775                 780                 


Gly Lys Asp Ala Glu His Arg Ala Ala Trp Arg Val Trp Cys Gln Lys 
785                 790                 795                 800 


Met Glu Lys Leu Ser Ala Leu Leu Thr Glu Asp Leu Arg Asp Asp Arg 
                805                 810                 815     


Val Val Val Met Ser Asn Val Arg Leu Arg Leu Gly Asn Gly Ser Ala 
            820                 825                 830         


His Lys Glu Thr Ile Gly Lys Leu Ser Lys Val Lys Leu Ser Ser Gln 
        835                 840                 845             


Leu Ser Val Ser Asp Ile Asp Lys Ala Ser Ser Glu Ala Leu Trp Cys 
    850                 855                 860                 


Ala Leu Thr Arg Glu Pro Gly Phe Asp Pro Lys Glu Gly Leu Pro Ala 
865                 870                 875                 880 


Asn Pro Glu Arg His Ile Arg Val Asn Gly Thr His Val Tyr Ala Gly 
                885                 890                 895     


Asp Asn Ile Gly Leu Phe Pro Val Ser Ala Gly Ser Ile Ala Leu Arg 
            900                 905                 910         


Gly Gly Tyr Ala Glu Leu Gly Ser Ser Phe His His Ala Arg Val Tyr 
        915                 920                 925             


Lys Ile Thr Ser Gly Lys Lys Pro Ala Phe Ala Met Leu Arg Val Tyr 
    930                 935                 940                 


Thr Ile Asp Leu Leu Pro Tyr Arg Asn Gln Asp Leu Phe Ser Val Glu 
945                 950                 955                 960 


Leu Lys Pro Gln Thr Met Ser Met Arg Gln Ala Glu Lys Lys Leu Arg 
                965                 970                 975     


Asp Ala Leu Ala Thr Gly Asn Ala Glu Tyr Leu Gly Trp Leu Val Val 
            980                 985                 990         


Asp Asp Glu Leu Val Val Asp Thr  Ser Lys Ile Ala Thr  Asp Gln Val 
        995                 1000                 1005             


Lys Ala  Val Glu Ala Glu Leu  Gly Thr Ile Arg Arg  Trp Arg Val 
    1010                 1015                 1020             


Asp Gly  Phe Phe Ser Pro Ser  Lys Leu Arg Leu Arg  Pro Leu Gln 
    1025                 1030                 1035             


Met Ser  Lys Glu Gly Ile Lys  Lys Glu Ser Ala Pro  Glu Leu Ser 
    1040                 1045                 1050             


Lys Ile  Ile Asp Arg Pro Gly  Trp Leu Pro Ala Val  Asn Lys Leu 
    1055                 1060                 1065             


Phe Ser  Asp Gly Asn Val Thr  Val Val Arg Arg Asp  Ser Leu Gly 
    1070                 1075                 1080             


Arg Val  Arg Leu Glu Ser Thr  Ala His Leu Pro Val  Thr Trp Lys 
    1085                 1090                 1095             


Val Gln  Lys Arg Pro Ala Ala  Thr Lys Lys Ala Gly  Gln Ala Lys 
    1100                 1105                 1110             


Lys Lys  Lys 
    1115     


<210>  1374
<211>  1016
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1374

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
            20                  25                  30          


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
        35                  40                  45              


Thr Lys Val Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
    50                  55                  60                  


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
65                  70                  75                  80  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
                85                  90                  95      


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
            100                 105                 110         


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
        115                 120                 125             


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
    130                 135                 140                 


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
145                 150                 155                 160 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
                165                 170                 175     


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
            180                 185                 190         


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
        195                 200                 205             


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
    210                 215                 220                 


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
225                 230                 235                 240 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
                245                 250                 255     


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
            260                 265                 270         


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
        275                 280                 285             


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
    290                 295                 300                 


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
305                 310                 315                 320 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
                325                 330                 335     


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
            340                 345                 350         


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
        355                 360                 365             


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
    370                 375                 380                 


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
385                 390                 395                 400 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
                405                 410                 415     


Leu Lys Leu Val Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
            420                 425                 430         


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
        435                 440                 445             


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
    450                 455                 460                 


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
465                 470                 475                 480 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
                485                 490                 495     


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
            500                 505                 510         


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
        515                 520                 525             


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
    530                 535                 540                 


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
545                 550                 555                 560 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
                565                 570                 575     


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
            580                 585                 590         


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
        595                 600                 605             


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
    610                 615                 620                 


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
625                 630                 635                 640 


Lys Asp Lys Glu Gln Lys Asn Phe Lys Asp Arg Asn Leu Asn Asp Thr 
                645                 650                 655     


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
            660                 665                 670         


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
        675                 680                 685             


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
    690                 695                 700                 


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
705                 710                 715                 720 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
                725                 730                 735     


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
            740                 745                 750         


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
        755                 760                 765             


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
    770                 775                 780                 


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
785                 790                 795                 800 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
                805                 810                 815     


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
            820                 825                 830         


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
        835                 840                 845             


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
    850                 855                 860                 


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
865                 870                 875                 880 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
                885                 890                 895     


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
            900                 905                 910         


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
        915                 920                 925             


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
    930                 935                 940                 


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
945                 950                 955                 960 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
                965                 970                 975     


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
            980                 985                 990         


Arg Gln Arg Glu Asp Phe Lys Lys  Lys Arg Pro Ala Ala  Thr Lys Lys 
        995                 1000                 1005             


Ala Gly  Gln Ala Lys Lys Lys  Lys 
    1010                 1015     


<210>  1375
<211>  1153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 variant product

<400>  1375

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ser Asp Leu Val Leu Gly Leu Asp Ile Gly Ile Gly Ser Val Gly 
            20                  25                  30          


Val Gly Ile Leu Asn Lys Val Thr Gly Glu Ile Ile His Lys Asn Ser 
        35                  40                  45              


Arg Ile Phe Pro Ala Ala Gln Ala Glu Asn Asn Leu Val Arg Arg Thr 
    50                  55                  60                  


Asn Arg Gln Gly Arg Arg Leu Ala Arg Arg Lys Lys His Arg Arg Val 
65                  70                  75                  80  


Arg Leu Asn Arg Leu Phe Glu Glu Ser Gly Leu Ile Thr Asp Phe Thr 
                85                  90                  95      


Lys Ile Ser Ile Asn Leu Asn Pro Tyr Gln Leu Arg Val Lys Gly Leu 
            100                 105                 110         


Thr Asp Glu Leu Ser Asn Glu Glu Leu Phe Ile Ala Leu Lys Asn Met 
        115                 120                 125             


Val Lys His Arg Gly Ile Ser Tyr Leu Asp Asp Ala Ser Asp Asp Gly 
    130                 135                 140                 


Asn Ser Ser Val Gly Asp Tyr Ala Gln Ile Val Lys Glu Asn Ser Lys 
145                 150                 155                 160 


Gln Leu Glu Thr Lys Thr Pro Gly Gln Ile Gln Leu Glu Arg Tyr Gln 
                165                 170                 175     


Thr Tyr Gly Gln Leu Arg Gly Asp Phe Thr Val Glu Lys Asp Gly Lys 
            180                 185                 190         


Lys His Arg Leu Ile Asn Val Phe Pro Thr Ser Ala Tyr Arg Ser Glu 
        195                 200                 205             


Ala Leu Arg Ile Leu Gln Thr Gln Gln Glu Phe Asn Pro Gln Ile Thr 
    210                 215                 220                 


Asp Glu Phe Ile Asn Arg Tyr Leu Glu Ile Leu Thr Gly Lys Arg Lys 
225                 230                 235                 240 


Tyr Tyr His Gly Pro Gly Asn Glu Lys Ser Arg Thr Asp Tyr Gly Arg 
                245                 250                 255     


Tyr Arg Thr Ser Gly Glu Thr Leu Asp Asn Ile Phe Gly Ile Leu Ile 
            260                 265                 270         


Gly Lys Cys Thr Phe Tyr Pro Asp Glu Phe Arg Ala Ala Lys Ala Ser 
        275                 280                 285             


Tyr Thr Ala Gln Glu Phe Asn Leu Leu Asn Asp Leu Asn Asn Leu Thr 
    290                 295                 300                 


Val Pro Thr Glu Thr Lys Lys Leu Ser Lys Glu Gln Lys Asn Gln Ile 
305                 310                 315                 320 


Ile Asn Tyr Val Lys Asn Glu Lys Ala Met Gly Pro Ala Lys Leu Phe 
                325                 330                 335     


Lys Tyr Ile Ala Lys Leu Leu Ser Cys Asp Val Ala Asp Ile Lys Gly 
            340                 345                 350         


Tyr Arg Ile Asp Lys Ser Gly Lys Ala Glu Ile His Thr Phe Glu Ala 
        355                 360                 365             


Tyr Arg Lys Met Lys Thr Leu Glu Thr Leu Asp Ile Glu Gln Met Asp 
    370                 375                 380                 


Arg Glu Thr Leu Asp Lys Leu Ala Tyr Val Leu Thr Leu Asn Thr Glu 
385                 390                 395                 400 


Arg Glu Gly Ile Gln Glu Ala Leu Glu His Glu Phe Ala Asp Gly Ser 
                405                 410                 415     


Phe Ser Gln Lys Gln Val Asp Glu Leu Val Gln Phe Arg Lys Ala Asn 
            420                 425                 430         


Ser Ser Ile Phe Gly Lys Gly Trp His Asn Phe Ser Val Lys Leu Met 
        435                 440                 445             


Met Glu Leu Ile Pro Glu Leu Tyr Glu Thr Ser Glu Glu Gln Met Thr 
    450                 455                 460                 


Ile Leu Thr Arg Leu Gly Lys Gln Lys Thr Thr Ser Ser Ser Asn Lys 
465                 470                 475                 480 


Thr Lys Tyr Ile Asp Glu Lys Leu Leu Thr Glu Glu Ile Tyr Asn Pro 
                485                 490                 495     


Val Val Ala Lys Ser Val Arg Gln Ala Ile Lys Ile Val Asn Ala Ala 
            500                 505                 510         


Ile Lys Glu Tyr Gly Asp Phe Asp Asn Ile Val Ile Glu Met Ala Arg 
        515                 520                 525             


Glu Thr Asn Glu Asp Asp Glu Lys Lys Ala Ile Gln Lys Ile Gln Lys 
    530                 535                 540                 


Ala Asn Lys Asp Glu Lys Asp Ala Ala Met Leu Lys Ala Ala Asn Gln 
545                 550                 555                 560 


Tyr Asn Gly Lys Ala Glu Leu Pro His Ser Val Phe His Gly His Lys 
                565                 570                 575     


Gln Leu Ala Thr Lys Ile Arg Leu Trp His Gln Gln Gly Glu Arg Cys 
            580                 585                 590         


Leu Tyr Thr Gly Lys Thr Ile Ser Ile His Asp Leu Ile Asn Asn Ser 
        595                 600                 605             


Asn Gln Phe Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp 
    610                 615                 620                 


Asp Ser Leu Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn Gln Glu 
625                 630                 635                 640 


Lys Gly Gln Arg Thr Pro Tyr Gln Ala Leu Asp Ser Met Asp Asp Ala 
                645                 650                 655     


Trp Ser Phe Arg Glu Leu Lys Ala Phe Val Arg Glu Ser Lys Thr Leu 
            660                 665                 670         


Ser Asn Lys Lys Lys Glu Tyr Leu Leu Thr Glu Glu Asp Ile Ser Lys 
        675                 680                 685             


Phe Asp Val Arg Lys Lys Phe Ile Glu Arg Asn Leu Val Asp Thr Arg 
    690                 695                 700                 


Tyr Ala Ser Arg Val Val Leu Asn Ala Leu Gln Glu His Phe Arg Ala 
705                 710                 715                 720 


His Lys Ile Asp Thr Lys Val Ser Val Val Arg Gly Gln Phe Thr Ser 
                725                 730                 735     


Gln Leu Arg Arg His Trp Gly Ile Glu Lys Thr Arg Asp Thr Tyr His 
            740                 745                 750         


His His Ala Val Asp Ala Leu Ile Ile Ala Ala Ser Ser Gln Leu Asn 
        755                 760                 765             


Leu Trp Lys Lys Gln Lys Asn Thr Leu Val Ser Tyr Ser Glu Asp Gln 
    770                 775                 780                 


Leu Leu Asp Ile Glu Thr Gly Glu Leu Ile Ser Asp Asp Glu Tyr Lys 
785                 790                 795                 800 


Glu Ser Val Phe Lys Ala Pro Tyr Gln His Phe Val Asp Thr Leu Lys 
                805                 810                 815     


Ser Lys Glu Phe Glu Asp Ser Ile Leu Phe Ser Tyr Gln Val Asp Ser 
            820                 825                 830         


Lys Phe Asn Arg Lys Ile Ser Asp Ala Thr Ile Tyr Ala Thr Arg Gln 
        835                 840                 845             


Ala Lys Val Gly Lys Asp Lys Ala Asp Glu Thr Tyr Val Leu Gly Lys 
    850                 855                 860                 


Ile Lys Asp Ile Tyr Thr Gln Asp Gly Tyr Asp Ala Phe Met Lys Ile 
865                 870                 875                 880 


Tyr Lys Lys Asp Lys Ser Lys Phe Leu Met Tyr Arg His Asp Pro Gln 
                885                 890                 895     


Thr Phe Glu Lys Val Ile Glu Pro Ile Leu Glu Asn Tyr Pro Asn Lys 
            900                 905                 910         


Gln Ile Asn Glu Lys Gly Lys Glu Val Pro Cys Asn Pro Phe Leu Lys 
        915                 920                 925             


Tyr Lys Glu Glu His Gly Tyr Ile Arg Lys Tyr Ser Lys Lys Gly Asn 
    930                 935                 940                 


Gly Pro Glu Ile Lys Ser Leu Lys Tyr Tyr Asp Ser Lys Leu Gly Asn 
945                 950                 955                 960 


His Ile Asp Ile Thr Pro Lys Asp Ser Asn Asn Lys Val Val Leu Gln 
                965                 970                 975     


Ser Val Ser Pro Trp Arg Ala Asp Val Tyr Phe Asn Lys Thr Thr Gly 
            980                 985                 990         


Lys Tyr Glu Ile Leu Gly Leu Lys  Tyr Ala Asp Leu Gln  Phe Glu Lys 
        995                 1000                 1005             


Gly Thr  Gly Thr Tyr Lys Ile  Ser Gln Glu Lys Tyr  Asn Asp Ile 
    1010                 1015                 1020             


Lys Lys  Lys Glu Gly Val Asp  Ser Asp Ser Glu Phe  Lys Phe Thr 
    1025                 1030                 1035             


Leu Tyr  Lys Asn Asp Leu Leu  Leu Val Lys Asp Thr  Glu Thr Lys 
    1040                 1045                 1050             


Glu Gln  Gln Leu Phe Arg Phe  Leu Ser Arg Thr Met  Pro Lys Gln 
    1055                 1060                 1065             


Lys His  Tyr Val Glu Leu Lys  Pro Tyr Asp Lys Gln  Lys Phe Glu 
    1070                 1075                 1080             


Gly Gly  Glu Ala Leu Ile Lys  Val Leu Gly Asn Val  Ala Asn Ser 
    1085                 1090                 1095             


Gly Gln  Cys Lys Lys Gly Leu  Gly Lys Ser Asn Ile  Ser Ile Tyr 
    1100                 1105                 1110             


Lys Val  Arg Thr Asp Val Leu  Gly Asn Gln His Ile  Ile Lys Asn 
    1115                 1120                 1125             


Glu Gly  Asp Lys Pro Lys Leu  Asp Phe Lys Arg Pro  Ala Ala Thr 
    1130                 1135                 1140             


Lys Lys  Ala Gly Gln Ala Lys  Lys Lys Lys 
    1145                 1150             


<210>  1376
<211>  1339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cpf1 variant product

<400>  1376

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
            20                  25                  30          


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
        35                  40                  45              


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
    50                  55                  60                  


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
65                  70                  75                  80  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
                85                  90                  95      


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
            100                 105                 110         


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
        115                 120                 125             


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
    130                 135                 140                 


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
145                 150                 155                 160 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
                165                 170                 175     


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
            180                 185                 190         


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
        195                 200                 205             


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
    210                 215                 220                 


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
225                 230                 235                 240 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
                245                 250                 255     


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
            260                 265                 270         


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
        275                 280                 285             


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
    290                 295                 300                 


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
305                 310                 315                 320 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
                325                 330                 335     


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
            340                 345                 350         


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
        355                 360                 365             


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
    370                 375                 380                 


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
385                 390                 395                 400 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
                405                 410                 415     


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
            420                 425                 430         


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
        435                 440                 445             


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
    450                 455                 460                 


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
465                 470                 475                 480 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
                485                 490                 495     


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
            500                 505                 510         


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
        515                 520                 525             


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
    530                 535                 540                 


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
545                 550                 555                 560 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
                565                 570                 575     


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
            580                 585                 590         


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
        595                 600                 605             


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
    610                 615                 620                 


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
625                 630                 635                 640 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
                645                 650                 655     


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
            660                 665                 670         


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
        675                 680                 685             


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
    690                 695                 700                 


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
705                 710                 715                 720 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
                725                 730                 735     


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
            740                 745                 750         


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
        755                 760                 765             


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
    770                 775                 780                 


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
785                 790                 795                 800 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
                805                 810                 815     


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
            820                 825                 830         


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
        835                 840                 845             


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
    850                 855                 860                 


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
865                 870                 875                 880 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
                885                 890                 895     


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
            900                 905                 910         


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
        915                 920                 925             


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
    930                 935                 940                 


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
945                 950                 955                 960 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
                965                 970                 975     


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
            980                 985                 990         


His Glu Ile Val Asp Leu Met Ile  His Tyr Gln Ala Val  Val Val Leu 
        995                 1000                 1005             


Glu Asn  Leu Asn Phe Gly Phe  Lys Ser Lys Arg Thr  Gly Ile Ala 
    1010                 1015                 1020             


Glu Lys  Ala Val Tyr Gln Gln  Phe Glu Lys Met Leu  Ile Asp Lys 
    1025                 1030                 1035             


Leu Asn  Cys Leu Val Leu Lys  Asp Tyr Pro Ala Glu  Lys Val Gly 
    1040                 1045                 1050             


Gly Val  Leu Asn Pro Tyr Gln  Leu Thr Asp Gln Phe  Thr Ser Phe 
    1055                 1060                 1065             


Ala Lys  Met Gly Thr Gln Ser  Gly Phe Leu Phe Tyr  Val Pro Ala 
    1070                 1075                 1080             


Pro Tyr  Thr Ser Lys Ile Asp  Pro Leu Thr Gly Phe  Val Asp Pro 
    1085                 1090                 1095             


Phe Val  Trp Lys Thr Ile Lys  Asn His Glu Ser Arg  Lys His Phe 
    1100                 1105                 1110             


Leu Glu  Gly Phe Asp Phe Leu  His Tyr Asp Val Lys  Thr Gly Asp 
    1115                 1120                 1125             


Phe Ile  Leu His Phe Lys Met  Asn Arg Asn Leu Ser  Phe Gln Arg 
    1130                 1135                 1140             


Gly Leu  Pro Gly Phe Met Pro  Ala Trp Asp Ile Val  Phe Glu Lys 
    1145                 1150                 1155             


Asn Glu  Thr Gln Phe Asp Ala  Lys Gly Thr Pro Phe  Ile Ala Gly 
    1160                 1165                 1170             


Lys Arg  Ile Val Pro Val Ile  Glu Asn His Arg Phe  Thr Gly Arg 
    1175                 1180                 1185             


Tyr Arg  Asp Leu Tyr Pro Ala  Asn Glu Leu Ile Ala  Leu Leu Glu 
    1190                 1195                 1200             


Glu Lys  Gly Ile Val Phe Arg  Asp Gly Ser Asn Ile  Leu Pro Lys 
    1205                 1210                 1215             


Leu Leu  Glu Asn Asp Asp Ser  His Ala Ile Asp Thr  Met Val Ala 
    1220                 1225                 1230             


Leu Ile  Arg Ser Val Leu Gln  Met Arg Asn Ser Asn  Ala Ala Thr 
    1235                 1240                 1245             


Gly Glu  Asp Tyr Ile Asn Ser  Pro Val Arg Asp Leu  Asn Gly Val 
    1250                 1255                 1260             


Cys Phe  Asp Ser Arg Phe Gln  Asn Pro Glu Trp Pro  Met Asp Ala 
    1265                 1270                 1275             


Asp Ala  Asn Gly Ala Tyr His  Ile Ala Leu Lys Gly  Gln Leu Leu 
    1280                 1285                 1290             


Leu Asn  His Leu Lys Glu Ser  Lys Asp Leu Lys Leu  Gln Asn Gly 
    1295                 1300                 1305             


Ile Ser  Asn Gln Asp Trp Leu  Ala Tyr Ile Gln Glu  Leu Arg Asn 
    1310                 1315                 1320             


Lys Arg  Pro Ala Ala Thr Lys  Lys Ala Gly Gln Ala  Lys Lys Lys 
    1325                 1330                 1335             


Lys 
    


<210>  1377
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cpf1 variant product

<400>  1377

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
            20                  25                  30          


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
        35                  40                  45              


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
    50                  55                  60                  


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
65                  70                  75                  80  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
                85                  90                  95      


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
            100                 105                 110         


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
        115                 120                 125             


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
    130                 135                 140                 


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
145                 150                 155                 160 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
                165                 170                 175     


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
            180                 185                 190         


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
        195                 200                 205             


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
    210                 215                 220                 


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
225                 230                 235                 240 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
                245                 250                 255     


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
            260                 265                 270         


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
        275                 280                 285             


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
    290                 295                 300                 


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
305                 310                 315                 320 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
                325                 330                 335     


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
            340                 345                 350         


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
        355                 360                 365             


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
    370                 375                 380                 


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
385                 390                 395                 400 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
                405                 410                 415     


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
            420                 425                 430         


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
        435                 440                 445             


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
    450                 455                 460                 


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
465                 470                 475                 480 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
                485                 490                 495     


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
            500                 505                 510         


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
        515                 520                 525             


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
    530                 535                 540                 


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
545                 550                 555                 560 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
                565                 570                 575     


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
            580                 585                 590         


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
        595                 600                 605             


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
    610                 615                 620                 


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
625                 630                 635                 640 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
                645                 650                 655     


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
            660                 665                 670         


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
        675                 680                 685             


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
    690                 695                 700                 


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
705                 710                 715                 720 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
                725                 730                 735     


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
            740                 745                 750         


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
        755                 760                 765             


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
    770                 775                 780                 


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
785                 790                 795                 800 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
                805                 810                 815     


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
            820                 825                 830         


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
        835                 840                 845             


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
    850                 855                 860                 


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
865                 870                 875                 880 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
                885                 890                 895     


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
            900                 905                 910         


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
        915                 920                 925             


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
    930                 935                 940                 


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
945                 950                 955                 960 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
                965                 970                 975     


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
            980                 985                 990         


Ile Lys Glu Met Lys Glu Gly Tyr  Leu Ser Gln Val Val  His Glu Ile 
        995                 1000                 1005             


Ala Lys  Leu Val Ile Glu Tyr  Asn Ala Ile Val Val  Phe Glu Asp 
    1010                 1015                 1020             


Leu Asn  Phe Gly Phe Lys Arg  Gly Arg Phe Lys Val  Glu Lys Gln 
    1025                 1030                 1035             


Val Tyr  Gln Lys Leu Glu Lys  Met Leu Ile Glu Lys  Leu Asn Tyr 
    1040                 1045                 1050             


Leu Val  Phe Lys Asp Asn Glu  Phe Asp Lys Thr Gly  Gly Val Leu 
    1055                 1060                 1065             


Arg Ala  Tyr Gln Leu Thr Ala  Pro Phe Glu Thr Phe  Lys Lys Met 
    1070                 1075                 1080             


Gly Lys  Gln Thr Gly Ile Ile  Tyr Tyr Val Pro Ala  Gly Phe Thr 
    1085                 1090                 1095             


Ser Lys  Ile Cys Pro Val Thr  Gly Phe Val Asn Gln  Leu Tyr Pro 
    1100                 1105                 1110             


Lys Tyr  Glu Ser Val Ser Lys  Ser Gln Glu Phe Phe  Ser Lys Phe 
    1115                 1120                 1125             


Asp Lys  Ile Cys Tyr Asn Leu  Asp Lys Gly Tyr Phe  Glu Phe Ser 
    1130                 1135                 1140             


Phe Asp  Tyr Lys Asn Phe Gly  Asp Lys Ala Ala Lys  Gly Lys Trp 
    1145                 1150                 1155             


Thr Ile  Ala Ser Phe Gly Ser  Arg Leu Ile Asn Phe  Arg Asn Ser 
    1160                 1165                 1170             


Asp Lys  Asn His Asn Trp Asp  Thr Arg Glu Val Tyr  Pro Thr Lys 
    1175                 1180                 1185             


Glu Leu  Glu Lys Leu Leu Lys  Asp Tyr Ser Ile Glu  Tyr Gly His 
    1190                 1195                 1200             


Gly Glu  Cys Ile Lys Ala Ala  Ile Cys Gly Glu Ser  Asp Lys Lys 
    1205                 1210                 1215             


Phe Phe  Ala Lys Leu Thr Ser  Val Leu Asn Thr Ile  Leu Gln Met 
    1220                 1225                 1230             


Arg Asn  Ser Lys Thr Gly Thr  Glu Leu Asp Tyr Leu  Ile Ser Pro 
    1235                 1240                 1245             


Val Ala  Asp Val Asn Gly Asn  Phe Phe Asp Ser Arg  Gln Ala Pro 
    1250                 1255                 1260             


Lys Asn  Met Pro Gln Asp Ala  Asp Ala Asn Gly Ala  Tyr His Ile 
    1265                 1270                 1275             


Gly Leu  Lys Gly Leu Met Leu  Leu Gly Arg Ile Lys  Asn Asn Gln 
    1280                 1285                 1290             


Glu Gly  Lys Lys Leu Asn Leu  Val Ile Lys Asn Glu  Glu Tyr Phe 
    1295                 1300                 1305             


Glu Phe  Val Gln Asn Arg Asn  Asn Lys Arg Pro Ala  Ala Thr Lys 
    1310                 1315                 1320             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1325                 1330         


<210>  1378
<211>  51
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS2 variant

<400>  1378
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc g                51


<210>  1379
<211>  48
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS4 variant

<400>  1379
aagcgccccg ccgccaccaa gaaggcgggc caggccaaga agaagaag                    48


<210>  1380
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1380
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg aguacaaggu gcccagcaag aaguucaagg ugcuggggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcaucugc      240

uaccugcagg agaucuucuc caacgagaug gccaaggucg acgacagcuu cuuccaccgg      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcgccaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccggaag      420

aagcucgugg acagcaccga caaggcggac cugcgccuga ucuaccucgc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

guggacaagc uguucaucca gcugguccag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gcggcgugga cgcgaaggcc auccuguccg cccgccugag caagucccgg      660

cgccucgaga accugaucgc ccagcugccg ggggagaaga agaacggccu cuucgggaac      720

cugaucgccc ugagccucgg ccugacgccc aacuucaagu ccaacuucga ccuggcggag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg ggugaacacc gagaucacca aggccccgcu guccgccagc      960

augaucaagc gcuacgacga gcaccaccag gaccucacgc ugcugaaggc ccucguccgg     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc agagcaagaa cggguacgcg     1080

ggcuacaucg acgggggcgc cucccaggag gaguucuaca aguucaucaa gccgauccug     1140

gagaagaugg acggcaccga ggagcuccug gugaagcuga accgcgagga ccugcuccgg     1200

aagcagcgca ccuucgacaa cgggagcauc ccccaccaga uccaccuggg cgagcugcac     1260

gccauccucc gccggcagga ggacuucuac cccuuccuga aggacaaccg cgagaagauc     1320

gagaagaucc ugaccuuccg gauccccuac uacguggggc cgcucgcccg cggcaacucc     1380

cgguucgccu ggaugacgcg caagagcgag gagaccauca cccccuggaa cuucgaggag     1440

gucguggaca agggggcguc cgcccagagc uucaucgagc ggaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu gcugcccaag cacucccucc uguacgagua cuucacgguc     1560

uacaacgagc ugaccaaggu gaaguacgug accgagggca ugcgcaagcc ggccuuccuc     1620

agcggcgagc agaagaaggc caucgucgac cugcuguuca agaccaaccg gaaggugacg     1680

gugaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacuc cgucgagauc     1740

agcggggugg aggaccgcuu caacgcgucc cugggcaccu accacgaccu gcucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucguc     1860

cucacccuga cccuguucga ggaccgggag augaucgagg agcgccucaa gacguacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc gccgguacac cggguggggc     1980

cgccucagcc ggaagcugau caacgggauc cgcgacaagc aguccggcaa gaccauccug     2040

gacuuccuca agagcgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

ucccugaccu ucaaggagga cauccagaag gcccagguga gcgggcaggg cgacucccuc     2160

cacgagcaca ucgccaaccu ggcggggagc cccgccauca agaagggcau ccugcagacg     2220

gucaaggugg uggacgagcu cgucaaggug auggggcgcc acaagcccga gaacaucgug     2280

aucgagaugg cccgcgagaa ccagaccacc cagaagggcc agaagaacuc ccgggagcgc     2340

augaagcgga ucgaggaggg caucaaggag cuggggagcc agauccugaa ggagcacccc     2400

gucgagaaca cccagcucca gaacgagaag cuguaccugu acuaccucca gaacggccgc     2460

gacauguacg uggaccagga gcuggacauc aaccggcuga gcgacuacga cguggaccac     2520

aucgucccgc aguccuuccu caaggacgac agcaucgaca acaaggugcu gacgcgcucc     2580

gacaagaacc ggggcaagag cgacaacgug cccuccgagg aggucgugaa gaagaugaag     2640

aacuacuggc gccagcugcu caacgccaag cugaucaccc agcggaaguu cgacaaccug     2700

accaaggccg agcgcggggg ccucagcgag cuggacaagg cgggguucau caagcggcag     2760

cugguggaga cccgccagau caccaagcac gucgcccaga uccucgacuc ccggaugaac     2820

acgaaguacg acgagaacga caagcugauc cgcgagguga aggugaucac ccucaagagc     2880

aagcucgucu ccgacuuccg gaaggacuuc caguucuaca aggugcgcga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gccguggucg gcaccgcgcu gaucaagaag     3000

uaccccaagc ucgagagcga guucguguac ggggacuaca agguguacga cguccggaag     3060

augaucgcca aguccgagca ggagaucggc aaggccaccg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacggagauc acccuggcga acggggagau ccgcaagcgg     3180

ccccugaucg agaccaacgg cgagaccggc gagaucgugu gggacaaggg gcgcgacuuc     3240

gccacggugc ggaagguccu cuccaugccc caggugaaca ucgugaagaa gaccgagguc     3300

cagaccggcg gguucagcaa ggaguccauc cugcccaagc gcaacagcga caagcugauc     3360

gcccgcaaga aggacuggga ccccaagaag uacggcgggu ucgacucccc caccguggcc     3420

uacagcgugc ucgucguggc caagguggag aagggcaaga gcaagaagcu gaaguccguc     3480

aaggagcugc ucggcaucac gaucauggag cggagcuccu ucgagaagaa cccgaucgac     3540

uuccuggagg cgaaggggua caaggaggug aagaaggacc ugaucaucaa gcuccccaag     3600

uacagccugu ucgagcugga gaacggccgc aagcggaugc ucgccuccgc cggggagcug     3660

cagaagggca acgagcuggc ccuccccagc aaguacguga acuuccugua ccuggccucc     3720

cacuacgaga agcucaaggg gagcccggag gacaacgagc agaagcagcu guucgucgag     3780

cagcacaagc acuaccugga cgagaucauc gagcagaucu ccgaguucag caagcgcgug     3840

auccucgcgg acgccaaccu ggacaaggug cuguccgccu acaacaagca ccgggacaag     3900

cccauccgcg agcaggccga gaacaucauc caccucuuca cccugaccaa ccugggcgcc     3960

cccgcggccu ucaaguacuu cgacaccacg aucgaccgga agcgcuacac cagcaccaag     4020

gagguccucg acgccacccu gauccaccag uccaucacgg gccuguacga gacccggauc     4080

gaccucagcc agcucggggg cgacuga                                         4107


<210>  1381
<211>  4890
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1381
augaacuuca agauccugcc caucgccauc gaccucggcg ugaagaacac cggggucuuc       60

agcgccuucu accagaaggg caccucccug gagcgccugg acaacaagaa cgggaaggug      120

uacgagcuca gcaaggacuc cuacacgcug cugaugaaca accggaccgc gcgccggcac      180

cagcgccggg gcaucgaccg caagcagcuc gugaagcggc uguucaagcu gaucuggacc      240

gagcagcuca accuggagug ggacaaggac acccagcagg ccaucagcuu ccuguucaac      300

cgccgggggu ucuccuucau cacggacggc uacagcccgg aguaccucaa caucgucccc      360

gagcagguca aggccauccu gauggacauc uucgacgacu acaacggcga ggacgaccug      420

gacuccuacc ucaagcuggc caccgagcag gagagcaaga ucuccgagau cuacaacaag      480

cugaugcaga agauccucga guucaagcug augaagcugu gcaccgacau caaggacgac      540

aaggugagca ccaagacgcu caaggagauc accuccuacg aguucgagcu gcuggccgac      600

uaccucgcga acuacagcga gucccugaag acccagaagu ucagcuacac cgacaagcag      660

gggaaccuga aggagcucuc cuacuaccac cacgacaagu acaacaucca ggaguuccug      720

aagcgccacg ccacgaucaa cgaccggauc cuggacaccc uccugaccga cgaccuggac      780

aucuggaacu ucaacuucga gaaguucgac uucgacaaga acgaggagaa gcuccagaac      840

caggaggaca aggaccacau ccaggcccac cuccaccacu ucguguucgc cgugaacaag      900

aucaagagcg agauggcguc cggcgggcgc caccggagcc aguacuucca ggagaucacc      960

aacgugcugg acgagaacaa ccaccaggag ggcuaccuga agaacuucug cgagaaccug     1020

cacaacaaga aguacuccaa ccugagcguc aagaaccucg ugaaccugau cgggaaccug     1080

agcaaccucg agcugaagcc ccugcgcaag uacuucaacg acaagaucca cgccaaggcc     1140

gaccacuggg acgagcagaa guucacggag accuacugcc acuggauccu cggcgagugg     1200

cgggugggcg ucaaggacca ggacaagaag gacggggcca aguacuccua caaggaccug     1260

ugcaacgagc ugaagcagaa ggugaccaag gccggccucg uggacuuccu gcuggagcug     1320

gaccccugcc gcaccauccc gcccuaccuc gacaacaaca accggaagcc gcccaagugc     1380

cagagccuga uccugaaccc caaguuccuc gacaaccagu accccaacug gcagcaguac     1440

cugcaggagc ugaagaagcu ccaguccauc cagaacuacc uggacagcuu cgagacggac     1500

cugaaggucc ucaaguccag caaggaccag cccuacuucg uggaguacaa guccagcaac     1560

cagcagaucg cguccgggca gcgcgacuac aaggaccugg acgcccggau ccugcaguuc     1620

aucuucgacc gcgugaaggc cagcgacgag cuccugcuga acgagaucua cuuccaggcc     1680

aagaagcuca agcagaaggc cuccagcgag cuggagaagc uggaguccag caagaagcuc     1740

gacgagguca ucgcgaacuc ccagcugagc cagauccuga agucccagca caccaacggc     1800

aucuucgagc agggcaccuu ccuccaccug gugugcaagu acuacaagca gcggcagcgc     1860

gcccgcgaca gccggcugua caucaugccc gaguaccgcu acgacaagaa gcuccacaag     1920

uacaacaaca ccgggcgguu cgacgacgac aaccagcugc ugacguacug caaccacaag     1980

ccgcgccaga agcgguacca gcuccugaac gaccuggccg gcgugcucca ggucuccccc     2040

aacuuccuga aggacaagau cgggagcgac gacgaccugu ucaucagcaa guggcucgug     2100

gagcacaucc gcggcuucaa gaaggccugc gaggacuccc ugaagaucca gaaggacaac     2160

cgggggcugc ucaaccacaa gaucaacauc gcccgcaaca ccaagggcaa gugcgagaag     2220

gagaucuuca accugaucug caagaucgag ggcagcgagg acaagaaggg gaacuacaag     2280

cacggccugg cguacgagcu cggggugcug cuguucggcg agcccaacga ggccuccaag     2340

cccgaguucg accggaagau caagaaguuc aacagcaucu acuccuucgc ccagauccag     2400

cagaucgccu ucgccgagcg caaggggaac gcgaacacgu gcgccgucug cagcgccgac     2460

aacgcccacc ggaugcagca gaucaagauc accgagccgg uggaggacaa caaggacaag     2520

aucauccucu ccgcgaaggc ccagcgccug cccgccaucc ccacccggau cgucgacggc     2580

gccgugaaga agauggccac cauccuggcg aagaacaucg uggacgacaa cuggcagaac     2640

aucaagcagg uccucagcgc caagcaccag cugcacaucc ccaucaucac cgaguccaac     2700

gccuucgagu ucgagccggc ccuggccgac gugaagggca agagccucaa ggaccgccgg     2760

aagaaggcgc uggagcgcau cucccccgag aacaucuuca aggacaagaa caaccggauc     2820

aaggaguucg ccaaggggau cagcgccuac uccggcgcca accugaccga cggggacuuc     2880

gacggcgcca aggaggagcu cgaccacauc aucccccgca gccacaagaa guacgggacg     2940

cugaacgacg aggcgaaccu gaucugcgug acccggggcg acaacaagaa caagggcaac     3000

cgcaucuucu gccuccgcga ccuggccgac aacuacaagc ugaagcaguu cgagaccacc     3060

gacgaccucg agaucgagaa gaagaucgcc gacacgaucu gggacgccaa caagaaggac     3120

uucaaguucg ggaacuaccg guccuucauc aaccugacgc cccaggagca gaaggcguuc     3180

cgccacgccc uguuccucgc cgacgagaac ccgaucaagc aggccgucau ccgggccauc     3240

aacaaccgca accggaccuu cgugaacggc acccagcgcu acuucgcgga ggugcuggcc     3300

aacaacaucu accugcgggc caagaaggag aaccucaaca ccgacaagau cagcuucgac     3360

uacuucggga uccccaccau cggcaacggc cgcgggaucg ccgagauccg gcagcuguac     3420

gagaaggucg acuccgacau ccaggccuac gcgaagggcg acaagcccca ggccagcuac     3480

agccaccuga ucgacgccau gcucgccuuc ugcaucgccg cggacgagca ccgcaacgac     3540

ggguccaucg gccuggagau cgacaagaac uacagccugu acccccucga caagaacacc     3600

ggggaggugu ucaccaagga caucuucucc cagaucaaga ucaccgacaa cgaguucagc     3660

gacaagaagc uggugcggaa gaaggccauc gagggcuuca acacgcaccg ccagaugacc     3720

cgggacggca ucuacgccga gaacuaccug ccgauccuca uccacaagga gcugaacgag     3780

guccgcaagg gguacaccug gaagaacucc gaggagauca agaucuucaa gggcaagaag     3840

uacgacaucc agcagcugaa caaccucgug uacugccuga aguucgugga caagcccauc     3900

agcaucgaca uccagaucuc cacccuggag gagcuccgga acauccugac gaccaacaac     3960

aucgccgcga ccgccgagua cuacuacauc aaccugaaga cccagaagcu ccacgaguac     4020

uacaucgaga acuacaacac ggcccugggg uacaagaagu acagcaagga gauggaguuc     4080

cugcgcuccc ucgccuaccg gagcgagcgc gucaagauca aguccaucga cgacgugaag     4140

cagguccugg acaaggacag caacuucauc aucggcaaga ucacccugcc cuucaagaag     4200

gaguggcagc ggcucuaccg cgaguggcag aacaccacca ucaaggacga cuacgaguuc     4260

cugaaguccu ucuucaacgu caagagcauc acgaagcugc acaagaaggu gcggaaggac     4320

uucucccucc cgaucagcac caacgagggg aaguuccugg ugaagcgcaa gaccugggac     4380

aacaacuuca ucuaccagau ccugaacgac uccgacagcc gcgccgacgg caccaagccc     4440

uucauccccg cguucgacau cuccaagaac gagaucgugg aggccaucau cgacagcuuc     4500

acgagcaaga acaucuucug gcuccccaag aacaucgagc ugcagaaggu ggacaacaag     4560

aacaucuucg ccaucgacac cuccaagugg uucgaggugg agaccccgag cgaccugcgg     4620

gacaucggca ucgccaccau ccaguacaag aucgacaaca acucccgccc caagguccgg     4680

gugaagcugg acuacgugau cgacgacgac agcaagauca acuacuucau gaaccacucc     4740

cuccucaaga gccgcuaccc cgacaagguc cuggagaucc ugaagcaguc cacgaucauc     4800

gaguucgaga gcuccggguu caacaagacc aucaaggaga ugcugggcau gaagcuggcc     4860

gggaucuaca acgagaccag caacaacuga                                      4890


<210>  1382
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1382
augaagcgca acuacauccu gggccucgac aucgggauca ccagcguggg cuacgggauc       60

aucgacuacg agacccggga cgugaucgac gccggcgugc gccuguucaa ggaggccaac      120

guggagaaca acgaggggcg gcgcuccaag cggggcgcgc gccggcugaa gcgccggcgc      180

cggcaccgca uccagcgggu caagaagcuc cuguucgacu acaaccugcu cacggaccac      240

agcgagcugu ccggcaucaa ccccuacgag gcccgcguga aggggcugag ccagaagcuc      300

uccgaggagg aguucagcgc cgcccugcug caccucgcca agcggcgcgg cgugcacaac      360

gucaacgagg uggaggagga caccgggaac gagcugucca ccaaggagca gaucagccgg      420

aacuccaagg cgcuggagga gaaguacgug gccgagcucc agcuggagcg ccugaagaag      480

gacggcgagg uccgggggag caucaaccgc uucaagaccu ccgacuacgu gaaggaggcc      540

aagcagcucc ugaaggugca gaaggccuac caccagcugg accagagcuu caucgacacg      600

uacaucgacc uccuggagac ccggcgcacc uacuacgagg gcccgggcga gggguccccc      660

uucggcugga aggacaucaa ggagugguac gagaugcuga uggggcacug caccuacuuc      720

cccgaggagc uccgcagcgu caaguacgcg uacaacgccg accuguacaa cgcccugaac      780

gaccucaaca accuggugau cacgcgggac gagaacgaga agcuggagua cuacgagaag      840

uuccagauca ucgagaacgu guucaagcag aagaagaagc ccacccucaa gcagaucgcc      900

aaggagaucc uggucaacga ggaggacauc aagggcuacc gcgugaccuc caccggcaag      960

ccggaguuca cgaaccugaa gguguaccac gacaucaagg acaucaccgc ccggaaggag     1020

aucaucgaga acgcggagcu ccuggaccag aucgccaaga uccugaccau cuaccagagc     1080

uccgaggaca uccaggagga gcucaccaac cugaacagcg agcugacgca ggaggagauc     1140

gagcagaucu ccaaccucaa gggguacacc ggcacccaca accugagccu gaaggccauc     1200

aaccucaucc uggacgagcu guggcacacc aacgacaacc agaucgccau cuucaaccgc     1260

cucaagcugg uccccaagaa gguggaccug agccagcaga aggagauccc cacgacccuc     1320

guggacgacu ucauccuguc ccccgucgug aagcggagcu ucauccaguc caucaaggug     1380

aucaacgcca ucaucaagaa guacgggcug ccgaacgaca ucaucaucga gcuggcgcgc     1440

gagaagaaca gcaaggacgc ccagaagaug aucaacgaga ugcagaagcg gaaccgccag     1500

accaacgagc ggaucgagga gaucauccgc accacgggca aggagaacgc caaguaccuc     1560

aucgagaaga ucaagcugca cgacaugcag gaggggaagu gccuguacuc ccucgaggcc     1620

aucccccugg aggaccugcu caacaacccc uucaacuacg aggucgacca caucaucccc     1680

cggagcgugu ccuucgacaa cagcuucaac aacaaggucc uggugaagca ggaggagaac     1740

uccaagaagg gcaaccgcac cccguuccag uaccugagcu ccagcgacuc caagaucagc     1800

uacgagaccu ucaagaagca cauccucaac cuggccaagg gcaaggggcg gaucuccaag     1860

accaagaagg aguaccugcu cgaggagcgc gacaucaacc gguucagcgu gcagaaggac     1920

uucaucaacc gcaaccuggu cgacacgcgg uacgcgaccc gcggccugau gaaccuccug     1980

cgguccuacu uccgcgugaa caaccuggac gugaagguca agagcaucaa cgggggcuuc     2040

accuccuucc uccgccggaa guggaaguuc aagaaggagc gcaacaaggg guacaagcac     2100

cacgccgagg acgcccugau caucgccaac gccgacuuca ucuucaagga guggaagaag     2160

cuggacaagg cgaagaaggu gauggagaac cagauguucg aggagaagca ggccgagagc     2220

augcccgaga ucgagaccga gcaggaguac aaggagaucu ucaucacgcc ccaccagauc     2280

aagcacauca aggacuucaa ggacuacaag uacagccacc ggguggacaa gaagcccaac     2340

cgcgagcuca ucaacgacac ccuguacucc acccggaagg acgacaaggg caacacccug     2400

aucgucaaca accucaacgg ccuguacgac aaggacaacg acaagcugaa gaagcucauc     2460

aacaagagcc cggagaagcu gcugauguac caccacgacc cccagacgua ccagaagcuc     2520

aagcugauca uggagcagua cggggacgag aagaaccccc uguacaagua cuacgaggag     2580

accggcaacu accucaccaa guacuccaag aaggacaacg ggcccgugau caagaagauc     2640

aaguacuacg gcaacaagcu gaacgcccac cuggacauca ccgacgacua cccgaacagc     2700

cgcaacaagg uggucaagcu cucccugaag cccuaccggu ucgacgugua ccuggacaac     2760

gggguguaca aguucgucac cgugaagaac cucgacguga ucaagaagga gaacuacuac     2820

gaggucaaca gcaagugcua cgaggaggcc aagaagcuga agaagaucuc caaccaggcg     2880

gaguucaucg ccagcuucua caacaacgac cugaucaaga ucaacggcga gcucuaccgc     2940

gugaucggcg ugaacaacga ccugcugaac cggaucgagg ucaacaugau cgacaucacg     3000

uaccgcgagu accucgagaa caugaacgac aagcggccgc cccgcaucau caagaccauc     3060

gccuccaaga cccagagcau caagaaguac uccaccgaca uccuggggaa ccuguacgag     3120

gucaagagca agaagcaccc ccagaucauc aagaagggcu ga                        3162


<210>  1383
<211>  4230
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1383
augcuguuca acaagugcau caucaucagc aucaaccucg acuucuccaa caaggagaag       60

ugcaugacca agcccuacag caucggccug gacaucggga ccaacuccgu gggcugggcc      120

gucaucacgg acaacuacaa ggugccgagc aagaagauga aggugcuggg gaacaccucc      180

aagaaguaca ucaagaagaa ccuccugggc guccugcugu ucgacagcgg gaucaccgcc      240

gagggccgcc ggcugaagcg caccgcgcgg cgccgguaca cgcgccggcg caaccggauc      300

cuguaccucc aggagaucuu cuccaccgag auggccaccc uggacgacgc cuucuuccag      360

cgccuggacg acagcuuccu cgugcccgac gacaagcggg acuccaagua ccccaucuuc      420

ggcaaccugg uggaggagaa ggucuaccac gacgaguucc ccaccaucua ccaccuccgc      480

aaguaccucg ccgacagcac gaagaaggcc gaccugcggc ugguguaccu cgcgcuggcc      540

cacaugauca aguaccgcgg gcacuuccug aucgagggcg aguucaacuc caagaacaac      600

gacauccaga agaacuucca ggacuuccuc gacaccuaca acgccaucuu cgagagcgac      660

cugucccugg agaacagcaa gcagcucgag gagaucguga aggacaagau cuccaagcug      720

gagaagaagg accggauccu gaagcuguuc ccgggggaga agaacagcgg caucuucucc      780

gaguuccuga agcugaucgu cgggaaccag gccgacuucc gcaagugcuu caaccucgac      840

gagaaggcga gccugcacuu cuccaaggag agcuacgacg aggaccugga gacccuccug      900

ggcuacaucg gcgacgacua cagcgacgug uuccugaagg ccaagaagcu cuacgacgcc      960

auccugcugu ccggguuccu caccgugacg gacaacgaga ccgaggcgcc ccugagcucc     1020

gccaugauca agcgguacaa cgagcacaag gaggaccugg cccuccugaa ggaguacauc     1080

cgcaacauca gccugaagac cuacaacgag gucuucaagg acgacaccaa gaacggcuac     1140

gccggguaca ucgacggcaa gacgaaccag gaggacuucu acguguaccu caagaaccug     1200

cuggccgagu ucgagggcgc cgacuacuuc cucgagaaga ucgaccggga ggacuuccug     1260

cgcaagcagc ggaccuucga caacgggucc auccccuacc agauccaccu ccaggagaug     1320

cgcgccaucc uggacaagca ggcgaaguuc uaccccuucc ucgccaagaa caaggagcgc     1380

aucgagaaga uccugaccuu ccggaucccg uacuacgugg gcccccuggc ccgcgggaac     1440

agcgacuucg ccugguccau ccggaagcgc aacgagaaga ucacccccug gaacuucgag     1500

gacgucaucg acaaggagag cuccgccgag gcguucauca accggaugac gagcuucgac     1560

cucuaccugc ccgaggagaa ggugcugccg aagcacuccc uccuguacga gaccuucaac     1620

guguacaacg agcugaccaa gguccgcuuc aucgccgaga gcaugcggga cuaccaguuc     1680

cucgacucca agcagaagaa ggacaucgug cgccuguacu ucaaggacaa gcggaaggug     1740

accgacaagg acaucaucga guaccugcac gccaucuacg gcuacgacgg gaucgagcuc     1800

aagggcaucg agaagcaguu caacagcucc cugagcacgu accacgaccu gcucaacauc     1860

aucaacgaca aggaguuccu ggacgacucc agcaacgagg ccaucaucga ggagaucauc     1920

cacacccuga ccaucuucga ggaccgcgag augaucaagc agcggcucuc caaguucgag     1980

aacaucuucg acaagagcgu ccugaagaag cugagccgcc ggcacuacac cggcuggggg     2040

aagcucuccg ccaagcugau caacggcauc cgcgacgaga aguccgggaa cacgauccug     2100

gacuaccuca ucgacgacgg caucagcaac cggaacuuca ugcagcugau ccacgacgac     2160

gcgcugagcu ucaagaagaa gauccagaag gcccagauca ucggggacga ggacaagggc     2220

aacaucaagg agguggugaa gucccucccc ggcagccccg ccaucaagaa ggggauccug     2280

caguccauca agaucgucga cgagcuggug aaggucaugg gcgggcgcaa gcccgagagc     2340

aucguggugg agauggcccg ggagaaccag uacaccaacc agggcaaguc caacagccag     2400

cagcgccuca agcggcugga gaagucccug aaggagcucg ggagcaagau ccugaaggag     2460

aacaucccgg cgaagcuguc caagaucgac aacaacgccc uccagaacga ccgccuguac     2520

cuguacuacc uccagaacgg caaggacaug uacaccggcg acgaccugga caucgaccgc     2580

cugagcaacu acgacaucga ccacaucauc ccccaggccu uccucaagga caacuccauc     2640

gacaacaagg uccuggugag cuccgccagc aaccggggga aguccgacga cuuccccagc     2700

cuggaggugg ucaagaagcg caagaccuuc ugguaccagc uccucaagag caagcugauc     2760

ucccagcgga aguucgacaa ccucacgaag gccgagcgcg gcgggcugcu gcccgaggac     2820

aaggcgggcu ucauccagcg gcagcucgug gagacccgcc agaucaccaa gcacguggcc     2880

cggcugcugg acgagaaguu caacaacaag aaggacgaga acaaccgcgc cguccggacc     2940

gugaagauca ucacgcucaa gagcacccug gugucccagu uccgcaagga cuucgagcug     3000

uacaaggucc gggagaucaa cgacuuccac cacgcccacg acgccuaccu caacgcggug     3060

aucgccagcg cccugcugaa gaaguacccg aagcucgagc ccgaguucgu guacggcgac     3120

uaccccaagu acaacuccuu ccgcgagcgc aagagcgcca ccgagaaggu cuacuucuac     3180

uccaacauca ugaacaucuu caagaagucc aucagccugg ccgacgggcg ggugaucgag     3240

cggccccuga ucgaggugaa cgaggagacc ggcgagagcg ucuggaacaa ggaguccgac     3300

cucgcgaccg ugcgccgggu gcugagcuac ccgcagguca acguggugaa gaaggucgag     3360

gagcagaacc acgggcugga ccgcggcaag cccaaggggc uguucaacgc caaccugucc     3420

agcaagccca agcccaacuc caacgagaac cuggugggcg ccaaggagua ccucgacccg     3480

aagaaguacg gcggguacgc cggcaucagc aacuccuucg cggugcuggu caaggggacg     3540

aucgagaagg gcgccaagaa gaagaucacc aacgugcugg aguuccaggg gaucagcauc     3600

cucgaccgga ucaacuaccg caaggacaag cugaacuucc ugcucgagaa gggcuacaag     3660

gacaucgagc ugaucaucga gcugcccaag uacagccucu ucgagcuguc cgacggcagc     3720

cggcgcaugc uggccuccau ccucagcacc aacaacaagc gcggggagau ccacaagggc     3780

aaccagaucu uccuguccca gaaguucgug aagcugcucu accacgccaa gcggaucagc     3840

aacaccauca acgagaacca ccgcaaguac gucgagaacc acaagaagga guucgaggag     3900

cuguucuacu acauccugga guucaacgag aacuacgugg gggccaagaa gaacggcaag     3960

cuccugaacu ccgcguucca gagcuggcag aaccacucca ucgacgagcu gugcagcucc     4020

uucaucgggc ccacgggcag cgagcggaag ggccucuucg agcugaccuc ccgcgggagc     4080

gccgccgacu ucgaguuccu gggcgugaag aucccgcggu accgcgacua cacccccucc     4140

agccuccuga aggacgccac ccugauccac caguccguca cggggcugua cgagacccgg     4200

aucgaccucg ccaagcuggg cgagggguga                                      4230


<210>  1384
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1384
augugguacg ccagccugau guccgcccac caccuccgcg ugggcaucga cgucgggacc       60

cacagcgugg gccuggcgac ccugcgggug gacgaccacg ggacgcccau cgagcuccug      120

uccgcccuga gccacaucca cgacuccggc gucgggaagg agggcaagaa ggaccacgac      180

acccgcaaga agcucagcgg caucgcccgg cgcgcccggc gccugcugca ccaccggcgc      240

acccagcucc agcagcugga cgaggugcug cgggaccucg gguucccgau ccccaccccc      300

ggcgaguucc uggaccugaa cgagcagacg gaccccuacc gcguguggcg gguccgcgcc      360

cggcucgugg aggagaagcu gccggaggag cugcgcgggc ccgcgaucuc cauggccgug      420

cggcacaucg cccgccaccg gggcuggcgc aaccccuaca gcaaggucga gucccuccug      480

agccccgccg aggagucccc guucaugaag gcgcugcggg agcgcauccu cgccaccacc      540

ggggaggugc uggacgacgg caucaccccc ggccaggcca uggcccaggu ggcccugacg      600

cacaacauca gcaugcgggg gcccgagggc auccucggga agcugcacca guccgacaac      660

gcgaacgaga uccgcaagau cugcgcccgc cagggcguca gccccgacgu gugcaagcag      720

cugcuccggg ccguguucaa ggccgacucc ccgcgcggca gcgccgucuc ccggguggcg      780

cccgaccccc ugcccgggca gggcagcuuc cgccgggccc cgaagugcga ccccgaguuc      840

cagcgcuucc ggaucaucuc caucguggcc aaccugcgca ucagcgagac caagggggag      900

aaccggcccc ucaccgccga cgagcgccgg cacgucguga ccuuccugac ggaggacagc      960

caggccgacc ugaccugggu ggacgucgcg gagaagcucg gcgugcaccg ccgggaccug     1020

cgcgggaccg ccgugcacac cgacgacggc gagcgguccg ccgcccggcc cccgaucgac     1080

gccacggacc ggaucaugcg ccagaccaag aucagcuccc ugaagaccug gugggaggag     1140

gcggacagcg agcagcgggg cgccaugauc cgcuaccucu acgaggaccc caccgacucc     1200

gagugcgccg agaucaucgc cgagcugccc gaggaggacc aggcgaagcu ggacagccuc     1260

caccuccccg ccgggcgcgc cgccuacucc cgggagagcc ugacggcccu cuccgaccac     1320

augcuggcga ccaccgacga ccugcacgag gcccgcaagc ggcuguucgg cgucgacgac     1380

agcugggccc cgcccgccga ggccaucaac gcgcccgugg ggaaccccuc cgucgaccgc     1440

acccugaaga ucgugggccg guaccugagc gccguggagu ccaugugggg gacgccggag     1500

gucauccacg uggagcacgu gcgcgacggc uucaccagcg agcggauggc cgacgagcgc     1560

gacaaggcca accggcgccg guacaacgac aaccaggagg ccaugaagaa gauccagcgc     1620

gacuacggca aggaggggua caucucccgg ggcgacaucg uccgccucga cgcgcuggag     1680

cugcaggggu gcgccugccu guacugcggc accaccaucg gguaccacac gugccagcuc     1740

gaccacaucg ugccccaggc cggccccggc agcaacaacc ggcgcgggaa ccugguggcc     1800

gucugcgagc ggugcaaccg cuccaagagc aacaccccgu ucgcggugug ggcccagaag     1860

ugcggcaucc cccacguggg ggucaaggag gccaucggcc gggugcgcgg cuggcggaag     1920

cagaccccca acaccuccag cgaggaccug acgcgccuca agaaggaggu gaucgcccgc     1980

cugcggcgca cccaggagga ccccgagauc gacgagcgga gcauggaguc cgucgccugg     2040

auggcgaacg agcugcacca ccgcaucgcc gccgccuacc cggagaccac cgugauggug     2100

uaccggggga gcaucacggc cgcggcccgc aaggccgccg gcaucgacuc ccggaucaac     2160

cucaucgggg agaagggccg caaggaccgg aucgaccgcc ggcaccacgc cgucgacgcg     2220

agcguggugg cccugaugga ggccuccguc gccaagaccc uggcggagcg cagcucccuc     2280

cgcggggagc agcgccugac cggcaaggag cagaccugga agcaguacac cggcagcacg     2340

gugggggccc gggagcacuu cgagaugugg cgcggccaca ugcugcaccu caccgagcug     2400

uucaacgagc ggcuggccga ggacaaggug uacgucaccc agaacauccg ccuccggcug     2460

uccgacggga acgcccacac cgugaacccc agcaagcugg ugucccaccg ccucggcgac     2520

gggcugacgg uccagcagau cgaccgcgcc ugcacccccg cgcuguggug cgcccucacc     2580

cgggagaagg acuucgacga gaagaacggc cugcccgccc gcgaggaccg ggccauccgc     2640

gugcacggcc acgagaucaa gagcuccgac uacauccagg uguucagcaa gcggaagaag     2700

accgacuccg accgcgacga gacgccguuc ggggccaucg cgguccgggg cggguucgug     2760

gagaucggcc ccagcaucca ccacgcccgc aucuaccggg uggaggggaa gaagcccguc     2820

uacgccaugc ugcgcguguu cacccacgac cuccuguccc agcggcacgg cgaccuguuc     2880

agcgccguga ucccgcccca gagcaucucc augcgcugcg ccgagcccaa gcuccggaag     2940

gcgaucacca ccggcaacgc cacguaccug gggugggucg uggugggcga cgagcuggag     3000

aucaacgucg acagcuucac caaguacgcc aucgggcgcu uccucgagga cuuccccaac     3060

accacccggu ggcgcaucug cggcuacgac acgaacucca agcugacccu gaagcccauc     3120

gugcucgccg ccgaggggcu ggagaacccg agcuccgcgg ugaacgagau cgucgagcug     3180

aagggcuggc ggguggccau caacgugcuc accaaggucc accccaccgu ggugcgccgg     3240

gacgcccugg gccgcccccg guacagcucc cgcagcaacc ugcccacguc cuggaccauc     3300

gaguga                                                                3306


<210>  1385
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1385
augaccaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

acgaccgaca acuacaaggu gccgagcaag aagaugaagg ugcucgggaa caccuccaag      120

aaguacauca agaagaaccu gcugggcguc cuccuguucg acagcgggau caccgccgag      180

ggccgccggc ugaagcgcac ggcgcggcgc cgguacaccc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucuc caccgagaug gccacccugg acgacgccuu cuuccagcgc      300

cucgacgaca gcuuccuggu gcccgacgac aagcgggacu ccaaguaccc caucuucggc      360

aaccuggugg aggagaaggc cuaccacgac gaguucccca cgaucuacca ccuccgcaag      420

uaccuggccg acagcaccaa gaaggcggac cugcggcucg ucuaccuggc ccuggcccac      480

augaucaagu accgcgggca cuuccucauc gagggcgagu ucaacuccaa gaacaacgac      540

auccagaaga acuuccagga cuuccuggac accuacaacg ccaucuucga gagcgaccug      600

ucccucgaga acagcaagca gcuggaggag aucgugaagg acaagaucuc caagcuggag      660

aagaaggacc ggauccucaa gcuguucccg ggggagaaga acagcggcau cuucuccgag      720

uuccugaagc ucaucguggg gaaccaggcg gacuuccgca agugcuucaa ccuggacgag      780

aaggccagcc ugcacuucuc caaggagagc uacgacgagg accucgagac ccugcugggc      840

uacaucggcg acgacuacuc cgacgucuuc cucaaggcca agaagcugua cgacgccauc      900

cugcucagcg gguuccugac ggugaccgac aacgagaccg aggccccgcu gagcuccgcg      960

augaucaagc gguacaacga gcacaaggag gaccucgccc ugcugaagga guacauccgc     1020

aacaucagcc ucaagaccua caacgaggug uucaaggacg acacgaagaa cggcuacgcc     1080

ggguacaucg acggcaagac caaccaggag gacuucuacg ucuaccugaa gaagcugcuc     1140

gccgaguucg agggcgccga cuacuuccug gagaagaucg accgggagga cuuccugcgc     1200

aagcagcgga ccuucgacaa cggguccauc cccuaccaga uccaccucca ggagaugcgc     1260

gcgauccugg acaagcaggc caaguucuac cccuuccugg ccaagaacaa ggagcgcauc     1320

gagaagaucc ugaccuuccg gaucccguac uacgugggcc cccucgcccg cgggaacagc     1380

gacuucgccu gguccauccg gaagcgcaac gagaagauca cgcccuggaa cuucgaggac     1440

gugaucgaca aggagagcuc cgcggaggcc uucaucaacc ggaugaccag cuucgaccug     1500

uaccugcccg aggagaaggu ccucccgaag cacucccugc uguacgagac cuucaacgug     1560

uacaacgagc ucaccaaggu gcgcuucauc gccgagagca ugcgggacua ccaguuccug     1620

gacuccaagc agaagaagga caucguccgc cuguacuuca aggacaagcg gaaggugacg     1680

gacaaggaca ucaucgagua ccuccacgcc aucuacggcu acgacgggau cgagcugaag     1740

ggcaucgaga agcaguucaa cagcucccug agcaccuacc acgaccuccu gaacaucauc     1800

aacgacaagg aguuccugga cgacuccagc aacgaggcca ucaucgagga gaucauccac     1860

acccucacca ucuucgagga ccgcgagaug aucaagcagc ggcuguccaa guucgagaac     1920

aucuucgaca agagcgugcu gaagaagcuc ucccgccggc acuacacggg cugggggaag     1980

cugagcgcga agcugaucaa cggcauccgc gacgagaagu ccgggaacac cauccucgac     2040

uaccugaucg acgacggcau cagcaaccgg aacuucaugc agcugaucca cgacgacgcc     2100

cucagcuuca agaagaagau ccagaaggcc cagaucaucg gggacgagga caagggcaac     2160

aucaaggagg ucgugaaguc ccugcccggc agccccgcca ucaagaaggg gauccugcag     2220

uccaucaaga ucguggacga gcucgucaag gugaugggcg ggcgcaagcc cgagagcauc     2280

gucguggaga uggcgcggga gaaccaguac accaaccagg gcaaguccaa cagccagcag     2340

cgccugaagc ggcuggagaa gucccucaag gagcugggga gcaagauccu gaaggagaac     2400

aucccggcca agcucuccaa gaucgacaac aacgcccugc agaacgaccg ccuguaccuc     2460

uacuaccugc agaacggcaa ggacauguac accggcgacg accuggacau cgaccgccuc     2520

agcaacuacg acaucgacca caucaucccc caggccuucc ugaaggacaa cuccaucgac     2580

aacaaggugc uggucagcuc cgccagcaac cgggggaagu ccgacgacgu gcccagccuc     2640

gaggugguca agaagcgcaa gacguucugg uaccagcugc ucaagagcaa gcucaucucc     2700

cagcggaagu ucgacaaccu gaccaaggcg gagcgcggcg ggcugagccc cgaggacaag     2760

gccggcuuca uccagcggca gcucguggag acccgccaga ucaccaagca cguggcccgg     2820

cugcuggacg agaaguucaa caacaagaag gacgagaaca accgcgccgu ccggacggug     2880

aagaucauca cccucaaguc cacccuggug agccaguucc gcaaggacuu cgagcuguac     2940

aagguccggg agaucaacga cuuccaccac gcccacgacg cguaccucaa cgccguggug     3000

gccuccgccc ugcugaagaa guacccgaag cucgagcccg aguucgucua cggcgacuac     3060

cccaaguaca acagcuuccg cgagcggaag uccgccaccg agaaggugua cuucuacagc     3120

aacaucauga acaucuucaa gaaguccauc agccuggcgg acgggcgcgu gaucgagcgg     3180

ccccugaucg aggucaacga ggagaccggc gaguccgugu ggaacaagga gagcgaccuc     3240

gccacggugc gccggguccu guccuacccc caggugaacg uggucaagaa gguggaggag     3300

cagaaccacg ggcuggaccg cggcaagccc aaggggcugu ucaacgccaa ccugagcucc     3360

aagcccaagc ccaacagcaa cgagaaccug gugggcgcca aggaguaccu cgacccgaag     3420

aaguacggcg gguacgcggg caucuccaac agcuucaccg uccuggugaa ggggaccauc     3480

gagaagggcg ccaagaagaa gaucaccaac gugcuggagu uccaggggau cuccauccuc     3540

gaccggauca acuaccgcaa ggacaagcug aacuuccugc ucgagaaggg cuacaaggac     3600

aucgagcuga ucaucgagcu gcccaaguac agccucuucg agcugagcga cggcucccgg     3660

cgcaugcugg ccagcauccu cuccacgaac aacaagcgcg gggagaucca caagggcaac     3720

cagaucuucc ugagccagaa guucgucaag cugcucuacc acgccaagcg gaucuccaac     3780

accaucaacg agaaccaccg caaguacgug gagaaccaca agaaggaguu cgaggagcug     3840

uucuacuaca uccuggaguu caacgagaac uacguggggg ccaagaagaa cggcaagcuc     3900

cugaacagcg cguuccaguc cuggcagaac cacagcaucg acgagcugug cuccagcuuc     3960

aucgggccca ccggcuccga gcggaagggc cucuucgagc ugaccagccg cggguccgcc     4020

gccgacuucg aguuccuggg cgucaagauc ccgcgguacc gcgacuacac gcccagcucc     4080

cuccugaagg acgccacccu gauccaccag agcgugaccg ggcucuacga gacccggauc     4140

gaccuggcca agcugggcga gggguga                                         4167


<210>  1386
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1386
auggccgccu ucaagcccaa cagcaucaac uacauccugg gccucgacau cgggaucgcg       60

uccgugggcu gggccauggu cgagaucgac gaggaggaga accccauccg ccugaucgac      120

cugggggugc ggguguucga gcgcgccgag guccccaaga ccggcgacag ccucgccaug      180

gcccggcgcc uggcgcgguc cgugcgccgg cugacccgcc ggcgcgccca ccggcuccug      240

cgcacgcggc gccugcucaa gcgggagggg gugcugcagg ccgccaacuu cgacgagaac      300

ggccugauca agagccuccc caacaccccc uggcagcugc gcgcggccgc ccuggaccgg      360

aagcucaccc cgcuggagug guccgccguc cugcuccacc ugaucaagca ccgcggcuac      420

cugagccagc ggaagaacga gggggagacc gccgacaagg agcucggcgc gcugcugaag      480

gggguggccg gcaacgccca cgcccuccag acgggggacu uccgcacccc cgccgagcug      540

gcgcugaaca aguucgagaa ggaguccggc cacauccgga accagcgcag cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaucc uccucuucga gaagcagaag      660

gaguucggca acccccacgu guccgggggc cugaaggagg ggaucgagac ccugcucaug      720

acgcagcggc ccgcccugag cggcgacgcc guccagaaga ugcugggcca cugcaccuuc      780

gagccggccg agcccaaggc ggccaagaac accuacaccg ccgagcgcuu caucuggcuc      840

acgaagcuga acaaccugcg gauccucgag cagggguccg agcgcccccu gaccgacacc      900

gagcgggcca cccugaugga cgagcccuac cgcaagagca agcucacgua cgcccaggcg      960

cggaagcugc ugggccucga ggacaccgcc uucuucaagg ggcugcgcua cggcaaggac     1020

aacgccgagg ccuccacccu gauggagaug aaggcguacc acgccaucag ccgggcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggca ccgccuucuc ccuguucaag acggacgagg acaucaccgg ccgccugaag     1200

gaccggaucc agcccgagau ccucgaggcc cugcugaagc acaucagcuu cgacaaguuc     1260

gugcagauca gccucaaggc gcugcgccgg aucgugcccc ugauggagca ggggaagcgc     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgga accccgucgu gcugcgcgcc     1440

cugucccagg cgcggaaggu gaucaacggc gucgugcgcc gguacgggag cccggcccgc     1500

auccacaucg agaccgcccg cgaggugggc aaguccuuca aggaccggaa ggagaucgag     1560

aagcgccagg aggagaaccg gaaggaccgc gagaaggccg ccgcgaaguu ccgggaguac     1620

uuccccaacu ucgucggcga gcccaagagc aaggacaucc ucaagcugcg ccuguacgag     1680

cagcagcacg ggaagugccu cuacuccggc aaggagauca accuggggcg gcugaacgag     1740

aagggcuacg uggagaucga ccacgcccuc cccuucagcc gcacguggga cgacuccuuc     1800

aacaacaagg ugcugguccu ggggagcgag aaccagaaca agggcaacca gaccccguac     1860

gaguacuuca acggcaagga caacucccgg gaguggcagg aguucaaggc ccgcguggag     1920

accagccggu ucccccgcuc caagaagcag cggauccucc ugcagaaguu cgacgaggac     1980

ggguucaagg agcgcaaccu gaacgacacc cgguacguga accgcuuccu cugccaguuc     2040

gucgccgacc ggaugcgccu gacgggcaag gggaagaagc ggguguucgc gagcaacggc     2100

cagaucacca accugcuccg cggcuucugg gggcugcgga agguccgcgc cgagaacgac     2160

cgccaccacg cccuggacgc cguggugguc gccugcucca ccguggcgau gcagcagaag     2220

aucacccggu ucgugcgcua caaggagaug aacgccuucg acggcaagac gaucgacaag     2280

gagaccgggg agguccucca ccagaagacc cacuuccccc agcccuggga guucuucgcc     2340

caggagguga ugauccgggu guucggcaag ccggacggga agcccgaguu cgaggaggcc     2400

gacacccugg agaagcugcg cacgcuccug gccgagaagc ugagcucccg gcccgaggcg     2460

guccacgagu acgugacccc gcuguucgug agccgcgccc ccaaccggaa gauguccggc     2520

cagggccaca uggagaccgu caagagcgcc aagcgccugg acgagggggu guccgugcug     2580

cggguccccc ucacccagcu gaagcugaag gaccucgaga agauggugaa ccgcgagcgg     2640

gagcccaagc uguacgaggc ccugaaggcc cgccucgagg cgcacaagga cgacccggcc     2700

aaggccuucg ccgagcccuu cuacaaguac gacaaggcgg gcaaccggac ccagcagguc     2760

aaggccgugc gcguggagca gguccagaag acgggggugu gggugcggaa ccacaacggc     2820

aucgccgaca acgccaccau ggugcgcguc gacguguucg agaaggggga caaguacuac     2880

cuggugccca ucuacagcug gcaggucgcc aagggcaucc ugcccgaccg ggcgguggug     2940

cagggcaagg acgaggagga cuggcagcuc aucgacgaca gcuucaacuu caaguucucc     3000

cugcacccga acgaccuggu cgaggugauc accaagaagg cccgcauguu cggguacuuc     3060

gccagcugcc accggggcac cgggaacauc aacauccgca uccacgaccu cgaccacaag     3120

aucggcaaga acgggauccu ggagggcauc ggcgugaaga cggcccuguc cuuccagaag     3180

uaccagaucg acgagcucgg gaaggagauc cgccccugcc ggcugaagaa gcgcccgccc     3240

guccgguga                                                             3249


<210>  1387
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1387
augaagaagc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacggacc aguacgaccu ggugaagcgc aagaugaaga ucgccgggga cuccgagaag      120

aagcagauca agaagaacuu cuggggcgug cggcuguucg acgaggggca gaccgcggcc      180

gaccgccgga uggcccgcac cgcccggcgc cggaucgagc gccggcgcaa ccggaucagc      240

uaccuccagg gcaucuucgc cgaggagaug uccaagaccg acgcgaacuu cuucugccgc      300

cugagcgacu ccuucuacgu cgacaacgag aagcggaaca gccgccaccc guucuucgcc      360

acgaucgagg aggaggugga guaccacaag aacuacccca ccaucuacca ccuccgggag      420

gagcugguga acuccagcga gaaggccgac cugcgccugg ucuaccucgc ccuggcgcac      480

aucaucaagu accggggcaa cuuccugauc gagggggccc ucgacaccca gaacaccucc      540

guggacggca ucuacaagca guucauccag acguacaacc agguguucgc cagcgggauc      600

gaggacggcu cccugaagaa gcuggaggac aacaaggacg ucgccaagau ccucguggag      660

aaggugaccc gcaaggagaa gcuggagcgg auccugaagc ucuaccccgg ggagaagucc      720

gccggcaugu ucgcgcaguu caucagccug aucgucggca gcaaggggaa cuuccagaag      780

cccuucgacc ugaucgagaa guccgacauc gagugcgcca aggacagcua cgaggaggac      840

cucgaguccc ugcuggcccu caucggcgac gaguacgccg agcuguucgu ggccgcgaag      900

aacgccuaca gcgccguggu ccuguccagc aucaucaccg uggccgagac cgagacgaac      960

gccaagcuca gcgcguccau gaucgagcgc uucgacaccc acgaggagga ccugggggag     1020

cugaaggccu ucaucaagcu ccaccucccg aagcacuacg aggagaucuu cagcaacacc     1080

gagaagcacg gcuacgccgg cuacaucgac gggaagacca agcaggccga cuucuacaag     1140

uacaugaaga ugacgcugga gaacaucgag ggcgccgacu acuucaucgc gaagaucgag     1200

aaggagaacu uccugcggaa gcagcgcacc uucgacaacg gggccauccc ccaccagcug     1260

caccuggagg agcucgaggc cauccugcac cagcaggcca aguacuaccc cuuccugaag     1320

gagaacuacg acaagaucaa gucccucgug accuuccgca uccccuacuu cgucggcccg     1380

cuggcgaacg ggcagagcga guucgccugg cugacccgga aggccgacgg cgagauccgc     1440

cccuggaaca ucgaggagaa gguggacuuc ggcaaguccg ccguggacuu caucgagaag     1500

augacgaaca aggacaccua ccuccccaag gagaacgucc ugcccaagca cagccugugc     1560

uaccagaagu accucgugua caacgagcug accaaggugc gguacaucaa cgaccagggg     1620

aagaccuccu acuucagcgg ccaggagaag gagcagaucu ucaacgaccu guucaagcag     1680

aagcgcaagg ucaagaagaa ggaccucgag cuguuccugc ggaacauguc ccacguggag     1740

agcccgacga ucgaggggcu ggaggacucc uucaacagcu ccuacagcac cuaccacgac     1800

cuccugaagg ucggcaucaa gcaggagauc cuggacaacc ccgugaacac cgagaugcuc     1860

gagaacaucg ugaagauccu gaccgucuuc gaggacaagc gcaugaucaa ggagcagcug     1920

cagcaguucu ccgacgugcu cgacggggug guccugaaga agcuggagcg gcgccacuac     1980

acgggcuggg gccggcucag cgccaagcug cugaugggga uccgcgacaa gcagucccac     2040

cucaccaucc uggacuaccu gaugaacgac gacggccuca accggaaccu gaugcagcug     2100

aucaacgaca gcaaccucuc cuucaagagc aucaucgaga aggagcaggu caccaccgcg     2160

gacaaggaca uccagagcau cguggccgac cuggccgggu cccccgccau caagaagggc     2220

auccugcaga gccucaagau cguggacgag cugguguccg ugauggggua cccgccccag     2280

acgaucgucg uggagauggc ccgcgagaac cagaccaccg gcaagggcaa gaacaacagc     2340

cggccccgcu acaagucccu ggagaaggcg aucaaggagu ucgggagcca gauccucaag     2400

gagcacccca ccgacaacca ggagcugcgg aacaaccgcc uguaccucua cuaccugcag     2460

aacggcaagg acauguacac ggggcaggac cuggacaucc acaaccucuc caacuacgac     2520

aucgaccaca ucgugcccca gagcuucauc accgacaacu ccaucgacaa ccugguccug     2580

accagcuccg ccggcaaccg ggagaagggc gacgacgugc cgccccucga gaucgugcgc     2640

aagcggaagg ucuucuggga gaagcuguac caggggaacc ugaugagcaa gcgcaaguuc     2700

gacuaccuca ccaaggccga gcgcggcggg cugaccgagg ccgacaaggc ccgguucauc     2760

caccgccagc ugguggagac gcggcagauc accaagaacg uggcgaacau ccuccaccag     2820

cgcuucaacu acgagaagga cgaccacggc aacaccauga agcagguccg gaucgugacc     2880

cugaaguccg cccuggugag ccaguuccgc aagcaguucc agcucuacaa gguccgggac     2940

gugaacgacu accaccacgc ccacgacgcc uaccugaacg ggguggucgc gaacacgcug     3000

cucaaggugu acccccagcu ggagcccgag uucguguacg gcgacuacca ccaguucgac     3060

ugguucaagg ccaacaaggc caccgccaag aagcaguucu acaccaacau caugcuguuc     3120

uucgcccaga aggaccgcau caucgacgag aacggcgaga uccucuggga caagaaguac     3180

cuggacaccg ucaagaaggu gauguccuac cggcagauga acaucgugaa gaagacggag     3240

auccagaagg gggaguucag caaggcgacc aucaagccga agggcaacuc cagcaagcug     3300

aucccccgca agaccaacug ggaccccaug aaguacgggg gccucgacag ccccaacaug     3360

gccuacgccg ucgugaucga guacgccaag gggaagaaca agcugguguu cgagaagaag     3420

aucauccggg ucaccaucau ggagcgcaag gccuucgaga aggacgagaa ggcguuccug     3480

gaggagcagg gcuaccggca gccgaaggug cucgccaagc ugcccaagua cacgcuguac     3540

gagugcgagg agggccgccg gcgcaugcuc gccuccgcca acgaggccca gaaggggaac     3600

cagcaggucc ugcccaacca ccuggugacc cuccugcacc acgcggccaa cugcgaggug     3660

agcgacggca agucccugga cuacaucgag agcaaccggg agauguucgc cgagcuccug     3720

gcccacgugu ccgaguucgc gaagcgcuac acccuggccg aggccaaccu caacaagauc     3780

aaccagcugu ucgagcagaa caaggagggg gacaucaagg ccaucgccca gagcuucguc     3840

gaccugaugg cguucaacgc caugggcgcc ccggccuccu ucaaguucuu cgagaccacg     3900

aucgagcgga agcgcuacaa caaccucaag gagcugcuga acagcaccau caucuaccag     3960

uccaucaccg ggcucuacga gagccggaag cgccuggacg acuga                     4005


<210>  1388
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1388
augaagaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

gugaccgacg acuacaaggu gccggccaag aagaugaagg uccucgggaa cacggacaag      120

agccacaucg agaagaaccu gcugggcgcg cuccuguucg acuccgggaa caccgccgag      180

gaccgccggc ugaagcgcac cgcccggcgc cgguacaccc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucag cgaggagaug ggcaaggugg acgacuccuu cuuccaccgc      300

cuggaggaca gcuuccucgu gacggaggac aagcggggcg agcgccaccc caucuucggg      360

aaccuggagg aggaggucaa guaccacgag aacuucccca ccaucuacca ccuccggcag      420

uaccuggccg acaaccccga gaagguggac cugcgccugg uguaccucgc ccuggcgcac      480

aucaucaagu uccggggcca cuuccugauc gaggggaagu ucgacacccg caacaacgac      540

guccagcgcc ucuuccagga guuccuggcc guguacgaca acaccuucga gaacuccagc      600

cugcaggagc agaacgugca ggucgaggag auccucacgg acaagaucuc caagagcgcc      660

aagaaggacc gggugcugaa gcuguucccg aacgagaagu ccaacggccg guucgccgag      720

uuccucaagc ugaucguggg gaaccaggcg gacuucaaga agcacuucga gcuggaggag      780

aaggccccgc uccaguucag caaggacacc uacgaggagg agcuggaggu ccugcucgcc      840

cagaucggcg acaacuacgc cgagcuguuc cuguccgcca agaagcucua cgacagcauc      900

cugcuguccg gcauccucac cgugaccgac guggggacga aggcgccccu gagcgccucc      960

augauccagc gcuacaacga gcaccagaug gaccuggccc agcucaagca guucauccgg     1020

cagaagcuga gcgacaagua caacgagguc uucuccgacg ugagcaagga cggcuacgcc     1080

ggguacaucg acggcaagac caaccaggag gccuucuaca aguaccugaa gggccuccug     1140

aacaagaucg aggggagcgg cuacuuccug gacaagaucg agcgcgagga cuuccuccgc     1200

aagcagcgga ccuucgacaa cggguccauc ccccaccaga uccaccucca ggagaugcgc     1260

gcgaucaucc ggcgccaggc cgaguucuac cccuuccugg ccgacaacca ggaccggauc     1320

gagaagcugc ugaccuuccg cauccccuac uacgugggcc cccuggcccg gggcaagagc     1380

gacuucgccu ggcugucccg caagagcgcg gacaagauca cgcccuggaa cuucgacgag     1440

aucgucgaca aggaguccag cgccgaggcc uucaucaacc ggaugaccaa cuacgaccuc     1500

uaccugccga accagaaggu gcugcccaag cacucccucc uguacgagaa guucaccgug     1560

uacaacgagc ugaccaaggu caaguacaag acggagcagg ggaagaccgc cuucuucgac     1620

gccaacauga agcaggagau cuucgacggc guguucaagg ucuaccgcaa ggugaccaag     1680

gacaagcuca uggacuuccu ggagaaggag uucgacgagu uccggaucgu ggaccugacc     1740

gggcucgaca aggagaacaa ggucuucaac gcgagcuacg gcacguacca cgaccugugc     1800

aagauccugg acaaggacuu ccucgacaac uccaagaacg agaagauccu ggaggacauc     1860

gugcugaccc ucacccuguu cgaggaccgc gagaugaucc ggaagcgccu ggagaacuac     1920

agcgaccucc ugaccaagga gcaggucaag aagcuggagc ggcgccacua cacggggugg     1980

ggccggcucu ccgccgagcu gauccacggg auccgcaaca aggagagccg gaagaccauc     2040

cuggacuacc ucaucgacga cggcaacucc aaccgcaacu ucaugcagcu gaucaacgac     2100

gacgcccuga gcuucaagga ggagaucgcc aaggcccagg ugaucggcga gaccgacaac     2160

cucaaccagg ugguguccga caucgcgggg agccccgcca ucaagaaggg cauccugcag     2220

ucccugaaga ucgucgacga gcucgugaag aucauggggc accagcccga gaacaucgug     2280

gucgagaugg cccgcgagaa ccaguucacc aaccagggcc ggcgcaacag ccagcagcgg     2340

cugaaggggc ugacggacuc caucaaggag uucggcagcc agauccucaa ggagcacccg     2400

guggagaaca gccagcugca gaacgaccgc cuguuccucu acuaccugca gaacggccgg     2460

gacauguaca ccggggagga gcuggacauc gacuaccucu cccaguacga caucgaccac     2520

aucauccccc aggccuucau caaggacaac agcaucgaca accgcgugcu gaccuccagc     2580

aaggagaacc ggggcaaguc cgacgacguc cccagcaagg acguggugcg caagaugaag     2640

uccuacugga gcaagcugcu cuccgcgaag cugaucaccc agcggaaguu cgacaaccug     2700

acgaaggccg agcgcggggg ccucaccgac gacgacaagg ccggcuucau caagcggcag     2760

cuggucgaga cccgccagau caccaagcac guggcccgga uccuggacga gcgcuucaac     2820

accgagacgg acgagaacaa caagaagauc cgccagguga agaucgucac ccucaagagc     2880

aaccuggugu ccaacuuccg gaaggaguuc gagcuguaca aggugcggga gaucaacgac     2940

uaccaccacg cccacgacgc guaccucaac gccgucaucg ggaaggcccu gcugggcgug     3000

uacccccagc ucgagccgga guucguguac ggggacuacc cccacuucca cggccacaag     3060

gagaacaagg ccaccgccaa gaaguucuuc uacagcaaca ucaugaacuu cuucaagaag     3120

gacgacgucc gcaccgacaa gaacggggag aucaucugga agaaggacga gcacaucucc     3180

aacaucaaga aggugcugag cuacccccag gugaacaucg ucaagaaggu ggaggagcag     3240

acgggcggcu ucuccaagga gagcauccug cccaagggga acuccgacaa gcucaucccg     3300

cggaagacca agaaguucua cugggacacc aagaaguacg gcggguucga cagccccauc     3360

guggcguaca gcauccuggu caucgccgac aucgagaagg gcaaguccaa gaagcugaag     3420

accgugaagg cccucguggg ggucacgauc auggagaaga ugaccuucga gcgcgacccc     3480

guggccuucc uggagcggaa gggcuaccgc aacgugcagg aggagaacau caucaagcug     3540

cccaaguaca gccucuucaa gcuggagaac ggccgcaagc ggcugcucgc guccgcccgc     3600

gagcugcaga aggggaacga gaucguccug ccgaaccacc ucggcacccu gcuguaccac     3660

gccaagaaca uccacaaggu ggacgagccc aagcaccucg acuacgugga caagcacaag     3720

gacgaguuca aggagcugcu ggacgucgug agcaacuucu ccaagaagua cacccucgcc     3780

gaggggaacc uggagaagau caaggagcug uacgcccaga acaacggcga ggaccucaag     3840

gagcuggcga gcuccuucau caaccugcuc acguucaccg ccaucggggc ccccgccacc     3900

uucaaguucu ucgacaagaa caucgaccgg aagcgcuaca ccagcacgac cgagauccug     3960

aacgccaccc ugauccacca guccaucacc ggccucuacg agacgcggau cgaccugaac     4020

aagcugggcg gggacuga                                                   4038


<210>  1389
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1389
augcagacca ccaaccugag cuacauccuc ggccuggacc uggggaucgc cuccgugggc       60

ugggccgucg uggagaucaa cgagaacgag gaccccaucg ggcucaucga cgugggcguc      120

cgcaucuucg agcgggcgga ggugccgaag acgggggaga gccuggcccu gucccgccgg      180

cucgcccgca gcacccggcg ccugauccgg cgccgggccc accgccugcu ccuggccaag      240

cgguuccuga agcgcgaggg cauccucucc accaucgacc uggagaaggg ccugcccaac      300

caggcguggg agcuccgggu ggccgggcug gagcgccggc ugagcgccau cgaguggggc      360

gccguccucc ugcaccugau caagcaccgc ggguaccucu ccaagcggaa gaacgagagc      420

cagaccaaca acaaggagcu gggcgcgcug cucuccgggg uggcccagaa ccaccagcug      480

cugcagagcg acgacuaccg cacgcccgcc gagcucgccc ugaagaaguu cgccaaggag      540

gagggccaca uccggaacca gcgcggcgcg uacacccaca ccuucaaccg gcuggaccuc      600

cuggccgagc ugaaccuccu guucgcccag cagcaccagu ucgggaaccc ccacugcaag      660

gagcacaucc agcaguacau gaccgagcug cucauguggc agaagccggc ccuguccggc      720

gaggccaucc ugaagaugcu cgggaagugc acgcacgaga agaacgaguu caaggcggcc      780

aagcacaccu acagcgccga gcgcuucgug uggcugacca agcugaacaa ccuccgcauc      840

cuggaggacg gcgccgagcg ggcccugaac gaggaggagc gccagcuccu gaucaaccac      900

cccuacgaga aguccaagcu gaccuacgcg cagguccgga agcuccuggg ccugagcgag      960

caggccaucu ucaagcaccu ccgcuacucc aaggagaacg ccgagagcgc cacguucaug     1020

gagcucaagg ccuggcacgc gauccggaag gcccuggaga accaggggcu gaaggacacc     1080

uggcaggacc ucgccaagaa gcccgaccug cuggacgaga ucggcaccgc cuucucccuc     1140

uacaagaccg acgaggacau ccagcaguac cugacgaaca aggugcccaa cagcgugauc     1200

aacgcgcugc ucgucucccu gaacuucgac aaguucaucg agcugagccu caagagccug     1260

cgcaagaucc ugccgcucau ggagcagggg aagcgguacg accaggccug ccgcgagauc     1320

uacggccacc acuacgggga ggccaaccag aagaccuccc agcugcugcc cgccaucccc     1380

gcccaggaga uccggaaccc cguggugcuc cgcacccuga gccaggcgcg gaaggucauc     1440

aacgccauca uccgccagua cggcuccccg gcccgggugc acaucgagac cggccgcgag     1500

cugggcaaga gcuucaagga gcggcgcgag auccagaagc agcaggagga caaccggacg     1560

aagcgcgagu ccgccgugca gaaguucaag gagcuguuca gcgacuucuc cagcgagccc     1620

aaguccaagg acauccugaa guuccggcug uacgagcagc agcacgggaa gugccucuac     1680

agcgggaagg agaucaacau ccaccgccug aacgagaagg gcuacgucga gaucgaccac     1740

gcccugcccu ucucccgcac cugggacgac agcuucaaca acaaggugcu cgugcuggcg     1800

uccgagaacc agaacaaggg gaaccagacc cccuacgagu ggcugcaggg caagaucaac     1860

agcgagcggu ggaagaacuu cgucgcccuc gugcugggcu cccagugcag cgccgccaag     1920

aagcagcgcc ugcucaccca ggugaucgac gacaacaagu ucaucgaccg gaaccugaac     1980

gacacgcgcu acaucgcccg guuccugucc aacuacaucc aggagaaccu ccugcugguc     2040

gggaagaaca agaagaacgu guucaccccg aacggccaga ucaccgcgcu ccugcgcagc     2100

cggugggggc ugaucaaggc ccgcgagaac aacaaccggc accacgcccu cgacgccauc     2160

gucguggcgu gcgccacccc cuccaugcag cagaagauca cgcgcuucau ccgguucaag     2220

gaggugcacc ccuacaagau cgagaaccgc uacgagaugg ucgaccagga gagcggcgag     2280

aucaucagcc cccacuuccc ggagcccugg gccuacuucc ggcaggaggu gaacauccgc     2340

guguucgaca accaccccga caccguccug aaggagaugc ugcccgaccg gccgcaggcc     2400

aaccaccagu ucgugcagcc ccucuucgug ucccgcgccc ccacccggaa gaugagcggg     2460

cagggccaca uggagaccau caaguccgcg aagcgccugg ccgagggcau cagcguccug     2520

cggauccccc ucacgcagcu gaagccgaac cugcucgaga acauggugaa caaggagcgc     2580

gagcccgccc uguacgccgg gcugaaggcc cggcucgcgg aguucaacca ggaccccgcc     2640

aaggccuucg ccaccccguu cuacaagcag ggcgggcagc aggucaaggc cauccgcgug     2700

gagcaggucc agaaguccgg cgugcuggug cgcgagaaca acggcguggc ggacaacgcc     2760

agcaucgugc ggaccgacgu cuucaucaag aacaacaagu ucuuccuggu gcccaucuac     2820

accuggcagg ucgccaaggg gauccucccc aacaaggcca ucguggcgca caagaacgag     2880

gacgaguggg aggagaugga cgagggcgcc aaguucaagu ucucccuguu ccccaacgac     2940

cugguggagc ucaagacgaa gaaggaguac uucuucgggu acuacaucgg ccuggaccgc     3000

gccaccggga acaucagccu gaaggagcac gacggcgaga ucuccaaggg caaggacggg     3060

guguaccggg ucggcgugaa gcucgcccug agcuucgaga aguaccaggu ggacgagcug     3120

gggaagaacc gccagaucug ccggccgcag cagcgccagc ccguccggug a              3171


<210>  1390
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1390
auggccgccu ucaagcccaa cccgaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggacgaga accccaucug ccugaucgac      120

cugggggugc gcguguucga gcgggccgag guccccaaga ccggcgacuc ccucgccaug      180

gcccgccggc uggcgcgcag cgugcggcgc cugacccggc gccgggccca ccgccuccug      240

cgggcccgcc ggcugcucaa gcgcgagggg gugcugcagg ccgcggacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc gggccgccgc ccuggaccgc      360

aagcucacgc cccuggagug gagcgccguc cugcuccacc ugaucaagca ccggggcuac      420

cugucccagc gcaagaacga gggggagacc gcggacaagg agcucggcgc ccugcugaag      480

gggguggccg acaacgccca cgcccuccag accggcgacu uccggacccc cgcggagcug      540

gcccugaaca aguucgagaa ggagagcggg cacauccgca accagcgggg cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaucc uccuguucga gaagcagaag      660

gaguucggca acccccacgu guccgggggc cugaaggagg ggaucgagac ccugcucaug      720

acccagcgcc cggcccugag cggcgacgcc guccagaaga ugcugggcca cugcacguuc      780

gagcccgcgg agcccaaggc cgccaagaac accuacaccg ccgagcgguu caucuggcuc      840

accaagcuga acaaccugcg cauccucgag cagggguccg agcggccccu gacggacacc      900

gagcgcgcca cccugaugga cgagccguac cgcaagagca agcucaccua cgcgcaggcc      960

cggaagcugc ugggccucga ggacacggcc uucuucaagg ggcugcggua cggcaaggac     1020

aacgccgagg cguccacccu gauggagaug aaggccuacc acgccaucag ccgcgcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggca ccgccuucuc ccuguucaag accgacgagg acaucacggg ccggcugaag     1200

gaccgcaucc agcccgagau ccucgaggcg cugcugaagc acaucagcuu cgacaaguuc     1260

gugcagaucu cccucaaggc ccugcggcgc aucgugcccc ugauggagca ggggaagcgg     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgca accccgucgu gcugcgggcg     1440

cugagccagg cccgcaaggu gaucaacggc gucgugcggc gcuacgggag ccccgcccgg     1500

auccacaucg agaccgcccg cgaggugggc aaguccuuca aggaccgcaa ggagaucgag     1560

aagcggcagg aggagaaccg caaggaccgg gagaaggccg cggccaaguu ccgcgaguac     1620

uuccccaacu ucgucggcga gcccaagagc aaggacaucc ucaagcugcg gcuguacgag     1680

cagcagcacg ggaagugccu cuacuccggc aaggagauca accuggggcg ccugaacgag     1740

aagggcuacg uggagaucga ccacgcccuc ccguucagcc ggaccuggga cgacuccuuc     1800

aacaacaagg ugcugguccu ggggagcgag aaccagaaca agggcaacca gacgcccuac     1860

gaguacuuca acggcaagga caacucccgc gaguggcagg aguucaaggc ccggguggag     1920

accagccgcu ucccccgguc caagaagcag cgcauccucc ugcagaaguu cgacgaggac     1980

ggguucaagg agcggaaccu gaacgacacc cgcuacguga accgguuccu cugccaguuc     2040

gucgcggacc gcaugcggcu gaccggcaag gggaagaagc gcguguucgc cagcaacggc     2100

cagaucacga accugcuccg gggcuucugg gggcugcgca agguccgggc cgagaacgac     2160

cgccaccacg cccuggacgc cguggugguc gcgugcucca ccguggccau gcagcagaag     2220

aucacccgcu ucgugcggua caaggagaug aacgccuucg acggcaagac caucgacaag     2280

gagacggggg agguccucca ccagaagacc cacuuccccc agccguggga guucuucgcc     2340

caggagguga ugauccgcgu guucggcaag cccgacggga agcccgaguu cgaggaggcc     2400

gacaccccgg agaagcugcg gacccugcuc gcggagaagc ugagcucccg ccccgaggcc     2460

guccacgagu acgugacgcc ccuguucgug agccgggccc ccaaccgcaa gauguccggc     2520

cagggccaca uggagaccgu caagagcgcc aagcggcucg acgagggggu guccgugcug     2580

cgcgucccgc ugacccagcu caagcugaag gaccuggaga agauggugaa ccgggagcgc     2640

gagcccaagc ucuacgaggc ccugaaggcg cggcuggagg cccacaagga cgaccccgcc     2700

aaggccuucg cggagcccuu cuacaaguac gacaaggccg gcaaccgcac ccagcagguc     2760

aaggccgugc ggguggagca gguccagaag accggggugu gggugcgcaa ccacaacggc     2820

aucgccgaca acgccacgau ggugcggguc gacguguucg agaaggggga caaguacuac     2880

cucgugccga ucuacagcug gcaggucgcg aagggcaucc ugcccgaccg cgccguggug     2940

cagggcaagg acgaggagga cuggcagcug aucgacgacu ccuucaacuu caaguucagc     3000

cuccacccca acgaccuggu cgaggugauc accaagaagg cccggauguu cggguacuuc     3060

gccagcugcc accgcggcac cgggaacauc aacauccgga uccacgaccu ggaccacaag     3120

aucggcaaga acgggauccu cgagggcauc ggcgugaaga ccgcccuguc cuuccagaag     3180

uaccagaucg acgagcuggg gaaggagauc cgccccugcc gccucaagaa gcggccgccc     3240

guccgcuga                                                             3249


<210>  1391
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1391
augaaguacc acgugggcau cgacgucggg accuucagcg ugggccuggc cgccaucgag       60

guggacgacg cggggaugcc caucaagacc cucucccugg ucagccacau ccacgacucc      120

ggccuggacc cggacgagau caagagcgcc gugacgcgcc ucgccuccag cgggaucgcc      180

cggcgcaccc ggcgccugua ccggcgcaag cggcgccggc ugcagcagcu cgacaaguuc      240

auccagcgcc agggcuggcc cgugaucgag cuggaggacu acuccgaccc ccuguacccc      300

uggaaggucc gggccgagcu cgcggccagc uacaucgccg acgagaagga gcgcggcgag      360

aagcuguccg uggcccugcg gcacaucgcg cgccaccggg gguggcgcaa cccguacgcc      420

aaggugagcu cccucuaccu gcccgacggc cccagcgacg ccuucaaggc cauccgggag      480

gagaucaagc gcgccuccgg gcagcccguc ccggagaccg cgaccguggg ccagauggug      540

acgcugugcg agcucgggac ccugaagcug cggggcgagg gcgggguccu cagcgcccgc      600

cugcagcagu ccgacuacgc ccgggagauc caggagaucu gccgcaugca ggagaucggc      660

caggagcugu accgcaagau caucgacgug guguucgccg ccgagagccc caaggggucc      720

gcgagcuccc gggucggcaa ggacccccuc cagcccggca agaaccgcgc ccugaaggcc      780

agcgacgccu uccagcggua ccgcaucgcc gcgcugaucg ggaaccuccg ggugcgcgug      840

gacggcgaga agcggauccu gagcgucgag gagaagaacc ugguguucga ccaccucgug      900

aaccugaccc cgaagaagga gcccgagugg gucaccaucg ccgagauccu ggggaucgac      960

cgcggccagc ucaucgggac ggccaccaug accgacgacg gcgagcgggc cggcgcccgc     1020

ccgcccaccc acgacacgaa ccgguccauc gugaacagcc gcaucgcgcc ccugguggac     1080

ugguggaaga ccgccuccgc ccuggagcag cacgccaugg ucaaggcgcu cagcaacgcc     1140

gagguggacg acuucgacuc ccccgagggg gccaaggucc aggccuucuu cgccgaccug     1200

gacgacgacg ugcacgcgaa gcuggacagc cuccaccucc ccgugggccg ggccgccuac     1260

uccgaggaca cccugguccg ccucacccgg cgcaugcuga gcgacggggu ggaccuguac     1320

acggcccggc uccaggaguu cggcaucgag cccuccugga ccccgcccac gccccgcauc     1380

ggggagcccg ugggcaaccc ggccgucgac cgggugcuga agaccgugag ccgcuggcug     1440

gaguccgcga ccaagaccug gggcgccccc gagcgcguca ucaucgagca cgugcgggag     1500

ggguucguga ccgagaagcg cgcccgggag auggacggcg acaugcgccg gcgcgccgcc     1560

cggaacgcga agcuguucca ggagaugcag gagaagcuga acguccaggg gaagcccagc     1620

cgcgccgacc uguggcggua ccaguccgug cagcgccaga acugccagug cgccuacugc     1680

ggcagcccca ucaccuucuc caacagcgag auggaccaca ucgugccgcg ggccgggcag     1740

ggcuccacga acacccgcga gaaccucguc gcggugugcc accggugcaa ccagagcaag     1800

ggcaacaccc ccuucgccau cugggccaag aacaccucca ucgagggggu gagcgucaag     1860

gaggccgugg agcgcacgcg gcacugggug accgacaccg gcaugcgcag caccgacuuc     1920

aagaaguuca cgaaggccgu cguggagcgg uuccagcgcg cgaccaugga cgaggagauc     1980

gacgcccggu ccauggagag cguggccugg auggccaacg agcugcgcuc ccgggucgcc     2040

cagcacuucg cgagccacgg gaccaccgug cgcguguacc gcggcucccu gacggccgag     2100

gcccggcgcg ccagcggcau cuccgggaag cugaaguucu ucgacggcgu cggcaagagc     2160

cggcucgacc gccggcacca cgccaucgac gcggccguga ucgccuucac cuccgacuac     2220

guggccgaga cccuggcggu ccgcagcaac cugaagcagu cccaggccca ccggcaggag     2280

gcgccccagu ggcgcgaguu caccgggaag gacgccgagc accgggccgc cuggcgcgug     2340

uggugccaga agauggagaa gcucagcgcc cugcugaccg aggaccuccg ggacgaccgc     2400

guggucguga uguccaacgu gcggcugcgc cuggggaacg gcagcgccca caaggagacg     2460

aucggcaagc ucuccaaggu caagcugagc ucccagcuga gcgugagcga caucgacaag     2520

gccuccagcg aggcccucug gugcgcgcug acccgggagc cgggguucga ccccaaggag     2580

ggccugcccg ccaaccccga gcgccacauc cgggugaacg ggacccacgu cuacgccggc     2640

gacaacaucg ggcuguuccc gguguccgcc ggcagcaucg cccugcgcgg cggguacgcg     2700

gagcugggcu ccagcuucca ccacgcccgg guguacaaga ucaccuccgg gaagaagccc     2760

gccuucgcca ugcuccgcgu cuacacgauc gaccugcugc ccuaccgcaa ccaggaccuc     2820

uucagcgugg agcugaagcc ccagaccaug uccaugcggc aggccgagaa gaagcugcgc     2880

gacgcgcucg ccaccggcaa cgccgaguac cugggguggc ugguggucga cgacgagcuc     2940

gugguggaca ccagcaagau cgccacggac caggucaagg cgguggaggc cgagcugggc     3000

accauccggc gcuggcgggu ggacggcuuc uucuccccga gcaagcugcg ccuccggccc     3060

cugcagaugu ccaaggaggg gaucaagaag gagagcgccc ccgagcuguc caagaucauc     3120

gaccgccccg gcuggcuccc ggccgucaac aagcuguuca gcgacgggaa cgugaccguc     3180

gugcggcgcg acucccuggg ccgggugcgc cucgagagca ccgcccaccu ccccgucacg     3240

uggaaggugc aguga                                                      3255


<210>  1392
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1392
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggucgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcccgg      180

cgcaaggcgc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggccu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcgcucaac gagcugcugu ccaagcagga cuucgcccgc      360

gugauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggccaucaag cagaacgagg agaagcuggc gaacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg uccggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggaggugcu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgucg gcaacugcuc cuucuucacc gacgagaagc gggcccccaa gaacagcccc      780

cuggcguuca uguucguggc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga ggugcugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg agcacaaccu gucccaggac gaccugaacg agaucgccaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcgcuggcca aguacgaccu caaccagaac     1140

cagaucgaca gccuguccaa gcuggaguuc aaggaccacc ucaacaucag cuucaaggcc     1200

cugaagcugg ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggccau caacgaggac aagaaggacu uccuccccgc guucaacgag     1320

accuacuaca aggacgaggu gacgaacccc gucgugcugc gggccaucaa ggaguaccgc     1380

aaggugcuga acgcccuccu gaagaaguac gggaaggucc acaagaucaa caucgagcug     1440

gcccgcgagg ugggcaagaa ccacucccag cgggcgaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

agcaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacucc     1620

ggggagaaga ucaagaucag cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuacu cccggagcuu cgacgacucc uacaugaaca aggugcuggu cuucaccaag     1740

cagaaccagg agaagcucaa ccagaccccg uucgaggccu ucggcaacga cagcgcgaag     1800

uggcagaaga ucgaggugcu ggccaagaac cugcccacca agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag aacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu gcucaacuac accaaggacu accuggacuu ccugccccuc     1980

uccgacgacg agaacaccaa gcugaacgac acccagaagg ggagcaaggu ccacguggag     2040

gccaaguccg gcaugcugac gagcgcccuc cgccacaccu ggggguucuc cgcgaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgugauca ucgccuacgc caacaacagc     2160

aucgucaagg cguucuccga cuucaagaag gagcaggaga gcaacagcgc cgagcuguac     2220

gccaagaaga ucuccgagcu ggacuacaag aacaagcgca aguucuucga gcccuucagc     2280

ggcuuccggc agaaggugcu ggacaagauc gacgagaucu ucgucuccaa gccggagcgc     2340

aagaagccca gcggcgcccu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

uccuacgggg gcaaggaggg ggugcucaag gcgcuggagc ugggcaagau ccgcaaggug     2460

aacgggaaga ucgucaagaa cggcgacaug uuccgggugg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu gcccaucuac acgauggacu ucgcccucaa gguccugccc     2580

aacaaggccg uggcccgcag caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucuc ccucuacaag gacagccuga uccugaucca gaccaaggac     2700

augcaggagc cggaguucgu guacuacaac gcguucaccu ccagcaccgu cucccucauc     2760

gugagcaagc acgacaacaa guucgagacg cuguccaaga accagaagau ccuguucaag     2820

aacgccaacg agaaggaggu gaucgccaag agcaucggga uccagaaccu caaggucuuc     2880

gagaaguaca ucguguccgc ccugggcgag gugaccaagg ccgaguuccg gcagcgcgag     2940

gacuucaaga aguga                                                      2955


<210>  1393
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1393
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucguccggcg caccaaccgg cagggccgcc ggcuggcccg ccggaagaag      180

caccgccggg ugcgccugaa ccgccucuuc gaggaguccg gccugaucac ggacuucacc      240

aagaucagca ucaaccugaa cccguaccag cuccggguga aggggcugac cgacgagcug      300

uccaacgagg agcuguucau cgcccugaag aacaugguca agcaccgggg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gaccaagacg cccgggcaga uccagcugga gcgcuaccag      480

accuacggcc agcugcgggg cgacuucacc gucgagaagg acgggaagaa gcaccgccuc      540

aucaacgugu uccccaccuc cgcguaccgg agcgaggccc ugcgcauccu gcagacgcag      600

caggaguuca acccccagau caccgacgag uucaucaacc gguaccucga gauccugacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggaccga cuacggccgc      720

uaccgcacga gcggggagac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuaccccg acgaguuccg ggccgccaag gcguccuaca ccgcccagga guucaaccug      840

cugaacgacc ucaacaaccu gacggugccc accgagacca agaagcugag caaggagcag      900

aagaaccaga ucaucaacua cgucaagaac gagaaggcca ugggccccgc caagcuguuc      960

aaguacaucg ccaagcuccu guccugcgac guggcggaca ucaaggggua ccgcaucgac     1020

aagagcggca aggccgagau ccacaccuuc gaggccuacc ggaagaugaa gacgcucgag     1080

acccuggaca ucgagcagau ggaccgcgag acccuggaca agcucgccua cgugcugacc     1140

cugaacacgg agcgggaggg cauccaggag gcccucgagc acgaguucgc ggacgggucc     1200

uucagccaga agcaggucga cgagcuggug caguuccgca aggccaacag cuccaucuuc     1260

ggcaaggggu ggcacaacuu cagcgugaag cugaugaugg agcucauccc ggagcuguac     1320

gagaccuccg aggagcagau gaccauccug acccggcucg gcaagcagaa gacgaccagc     1380

uccagcaaca agaccaagua caucgacgag aagcugcuga ccgaggagau cuacaacccc     1440

gucguggcca aguccgugcg ccaggccauc aagaucguca acgccgcgau caaggaguac     1500

ggggacuucg acaacaucgu gaucgagaug gcccgggaga cgaacgagga cgacgagaag     1560

aaggccaucc agaagaucca gaaggccaac aaggacgaga aggacgccgc gaugcucaag     1620

gccgccaacc aguacaacgg caaggccgag cugccccaca gcguguucca cggccacaag     1680

cagcuggcga ccaagauccg ccuguggcac cagcaggggg agcggugccu cuacaccggc     1740

aagaccaucu ccauccacga ccugaucaac aacagcaacc aguucgaggu cgaccacauc     1800

cugccccucu ccaucacguu cgacgacagc cuggccaaca aggugcuggu cuacgccacc     1860

gccaaccagg agaaggggca gcgcaccccg uaccaggccc ucgacuccau ggacgacgcg     1920

uggagcuucc gggagcugaa ggccuucgug cgcgagucca agacccugag caacaagaag     1980

aaggaguacc uccugaccga ggaggacauc uccaaguucg acgugcggaa gaaguucauc     2040

gagcgcaacc uggucgacac gcgguacgcc agccgcgugg ugcucaacgc ccugcaggag     2100

cacuuccggg cccacaagau cgacaccaag gucuccgugg ugcgcggcca guucaccagc     2160

cagcugcgcc ggcacugggg gaucgagaag acccgcgaca cguaccacca ccacgcgguc     2220

gacgcccuca ucaucgccgc cagcucccag cugaaccugu ggaagaagca gaagaacacc     2280

cucgugagcu acuccgagga ccagcugcug gacaucgaga ccggcgagcu caucagcgac     2340

gacgaguaca aggaguccgu guucaaggcc cccuaccagc acuucgucga cacccucaag     2400

agcaaggagu ucgaggacuc cauccuguuc agcuaccagg uggacuccaa guucaaccgg     2460

aagaucagcg acgcgacgau cuacgccacc cgccaggcca aggugggcaa ggacaaggcc     2520

gacgagaccu acguccucgg gaagaucaag gacaucuaca cccaggacgg cuacgacgcg     2580

uucaugaaga ucuacaagaa ggacaagucc aaguuccuga uguaccggca cgacccccag     2640

acguucgaga aggugaucga gcccauccug gagaacuacc cgaacaagca gaucaacgag     2700

aaggggaagg aggugcccug caaccccuuc cucaaguaca aggaggagca cggcuacauc     2760

cgcaaguaca gcaagaaggg caacgggccc gagaucaagu cccugaagua cuacgacagc     2820

aagcugggca accacaucga caucaccccg aaggacucca acaacaaggu cgugcuccag     2880

agcguguccc ccuggcgggc cgacgucuac uucaacaaga ccaccgggaa guacgagauc     2940

cugggccuga aguacgccga ccuccaguuc gagaagggga cgggcaccua caagaucagc     3000

caggagaagu acaacgacau caagaagaag gagggcgugg acagcgacuc cgaguucaag     3060

uucacccugu acaagaacga ccugcuccug gugaaggaca ccgagacgaa ggagcagcag     3120

cuguuccgcu uccucagccg gaccaugccc aagcagaagc acuacgucga gcugaagccc     3180

uacgacaagc agaaguucga gggcggggag gcccugauca aggugcucgg gaacguggcc     3240

aacuccggcc agugcaagaa ggggcugggc aagagcaaca ucuccaucua caagguccgc     3300

accgacgugc ugggcaacca gcacaucauc aagaacgagg gggacaagcc gaagcucgac     3360

uucuga                                                                3366


<210>  1394
<211>  2556
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1394
augaccaacg gcaagauccu ggggcucgac aucggcgugg ccagcgucgg ggugggcauc       60

aucgaggcca agaccgggaa ggugauccac gcgaacuccc gccuguucag cgccgccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcgcgc gccggcugac gcgccggaag      180

aagcaccgcg ucaagcgggu gcgcgaccuc uucgagaagu acgacauccu gaccgacuuc      240

cggaaccuga accucaaccc cuacgagcug cgcgugaagg ggcugaccga gcagcucacc      300

aacgaggagc uguucgccgc ccugcggacg aucgccaagc gccggggcau cuccuaccuc      360

gacgacgcgg aggacgacag caccgggucc agcgacuacg ccaaguccau cgacgagaac      420

cgccggcugc ucaagagcau gacccccggc cagauccagc uggagcgccu ggagaaguac      480

gggcagcugc ggggcaacuu caccgucuac gacgagaacg gcgaggccca ccgccucauc      540

aacguguucu ccacgagcga cuacaagaac gaggcccgga agauccugga gacccagucc      600

aacuacaaca agcagaucac cgacgaguuc aucgaggacu acaucgagau ccugacccag      660

aagcgcaagu acuaccacgg gcccggcaac gagaaguccc gcacggacua cgggcgguuc      720

cgcaccgacg gcaccacccu cgagaacauc uucggcaucc ugaucgggaa gugcagcuuc      780

uaccccgagg aguaccgggc cagcaaggcg uccuacacgg cccaggaguu caacuuccug      840

aacgaccuca acaaccugaa ggugcccacc gagaccggca agcugagcac cgagcagaag      900

gaguaccucg ucaacuucgc caagaacucc aagacgcugg gggccagcaa gcugcucaag      960

gagaucgcca agcuggugga cggcgacgug aaggacaucu ccggguaccg cgaggacagc     1020

aagggcaagc cggaccugca caccuucgag cccuaccgga agcucaaguu caaccugacc     1080

accgucgaca ucgacaaccu gagccgcgac auccucgaca agcuggcgga cauccugacg     1140

cucaacaccg agcgggaggg caucgaggac gccaucaacc agaaccugcc cgagcaguuc     1200

accaaggagc agaucuccga gaucgugcag auccgcaaga gccaguccag cgccuucaac     1260

aagggguggc acuccuucag cgccaagcug augaacgagc ucauccccga gcuguacgcc     1320

accuccgagg agcagaugac gauccugacc cggcucgaga aguucaaggc gaccaagaag     1380

uccagcaaga acaccaagac gaucgacgag aaggagauca ccgacgagau cuacaacccg     1440

guggucgcca agagcgugcg ccagaccauc aagaucauca acgccgccgu gaagaaguac     1500

ggcgacuucg acaagaucgu caucgagaug ccccgggaca agaacgccga cgacgagaag     1560

aaguucaucg acaagaagaa caaggagaac aagaaggaga aggacgacuc ccugaagcgc     1620

gcggccuacc uguacaacgg gaccgacaag cuccccgacg acguguucca cggcaacaag     1680

cagcuggcca cgaagauccg gcugugguac cagcaggggg agcgcugccu guacagcggc     1740

aagcccaucc ucauccagga ccuggugcac aacuccaaca acuucgagau cgaccacauc     1800

cugccgcuca gccuguccuu cgacgacagc cuggccaaca agguccucgu guacgcgugg     1860

gccaaccagg agaaggagca gaagaccccc uaccagguga ucgacuccau ggacgccgcc     1920

uggagcuucc gggagaugaa ggacuacguc cugaagcaga agggcaucgg gaagaagaag     1980

cgcgaguacc ugcucaccac cgagaacauc gacaagaucg aggugaagaa gaaguucauc     2040

gagcggaacc uggucgacac ccgcuacgcc ucccgggugg ugcugaacag ccuccagacg     2100

gcgcugaagg agcugggcaa ggacaccaag gucagcgugg ugcgcgggca guucaccucc     2160

cagcuccgcc ggaaguggaa gaucgacaag agccgcgaga ccuaccacca ccacgccguc     2220

gacgcccuga ucaucgccgc cuccagccag cugaagcucu ggcagaagca cgagaaccug     2280

auguucgaga acuacggcga gaaccaggug gugaacaagg agacggggga gauccugucc     2340

aucagcgacg acgaguacaa ggaguccguc uuccagccgc ccuaccaggg cuucgugaac     2400

accaucagcu ccaaggcguu cgaggacgag auccucuuca gcuaccaggu ggacuccaag     2460

uucaaccgga aggucagcga cgccaccauc uacuccaccc gcaaggccaa gcugggcaag     2520

gacaagaagg aggagacgua cgugcugggg aaguga                               2556


<210>  1395
<211>  2598
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1395
augcgccaca agaucggccu ggacaucggg aucaccagcg ugggcugggc cgucaucaac       60

cucgacaucc cccggaucga ggaccugggg gugcgcaucu ucgaccgggc cgagaacccc      120

cagaccggcg agucccuggc gcucccccgc cggcuggccc gcagcgcccg gcgccggcug      180

cgccggcgca agcaccggcu cgagcgcauc cggcgccugc ugauccggga ggggauccuc      240

acgaaggagg agcuggacaa gcuguucgag gagaagcacg agaucgacgu guggcagcuc      300

cgcgucgagg cccuggaccg gaagcugaac aacgacgagc ucgcccgcgu gcugcugcac      360

cucgcgaagc ggcgcggcuu caaguccaac cgcaagagcg agcggaacaa caaggagaac      420

uccaccaugc ugaagcacau cgaggagaac cgcgcccugc ucagcggcua ccggaccgug      480

gccgagauga ucgucaagga ccccaaguuc gccuuccaca agcgcaacaa gggggagaac      540

uacaccaaca cgaucgcgcg ggacgaccug gagcgcgaga ucaagcugau cuucaccaag      600

cagcgggagu acggcaacau cgugugcacc gagaccuucg agaacgagua caucaugauc      660

ugggccuccc agcgccccuu cgccagcaag aacgacaucg agaagaaggu gggguucugc      720

acguucgagc cgaaggagaa gcgggccccc aaggccaccu acaccuucca guccuucguc      780

gcgugggagc acaucaacaa gcuccgccug guguacccca ccggcacgcg ggggcugacc      840

gacgaggagc gccggcuccu guacaagcag gccuuccaca agaacaagau caccuaccac      900

gacauccgca cccugcucca ccuccccgac gacacguacu ucaagggcau cguguacgac      960

cggggcgccc cgcugaagca gagcgagacc auccgcuucc uggagcugga cgccuaccac     1020

cagauccgga aggccgucga caagguguac gggaagggca aguccagcuc cuuccugccc     1080

aucgacuucg acaccuucgg guacgcgcuc acccuguuca aggacgacgc cgacauccgc     1140

agcuaccugc ggaacgagua cgagcagaac ggcaagcgca ugcccaaccu cgccaacaag     1200

guguacgaca acgagcccau cgaggagcug cugaaccucu ccuucacgaa guucggccac     1260

cugagccuga aggcccuccg caacauccug ccguacaugg agcaggggga ggucuacucc     1320

guggccugcg agcgggcggg cuacaccuuc accgggccca agaagaagca gaagacccug     1380

cuccugccca acaucccgcc caucgccaac cccgugguca ugcgcgcccu gacgcaggcc     1440

cggaaggugg ugaacgccau caucaagaag uacggcagcc ccgucuccau ccacaucgag     1500

cuggcgcgcg accucagcca gaccuucgac gagcggcgca agaccaagcg ggagcaggac     1560

gagaaccgca agaagaacga gaccgccauc cggcagcuga uggaguacgg gcugacgauc     1620

aaccccaccg gccacgacau cgugaaguuc aagcucugga gcgagcagaa cggccgcugc     1680

gccuacuccc ugcagccgau cgagaucgag cggcugcucg agcccgggua cguggagguc     1740

gaccacguga uccccuacag ccgcucccug gacgacagcu acaccaacaa gguccuggug     1800

cucaccaagg agaaccggga gaagggcaac cgcauccccg ccgaguaccu gggggugggc     1860

acggagcggu ggcagcaguu caagaccuuc guccugacca acaagcaguu cuccaagaag     1920

aagcgcgacc ggcuccugcg ccugcacuac gacgagaacg aggaggcgga guggaagaac     1980

cggaaccuca acgacacccg cuacaucagc cgguucuucg ccaacuucau ccgcgagcac     2040

cugaaguucg ccgaguccga cgacaagcag aagguguaca cggugaacgg gcgcgucacc     2100

gcccaccucc ggagccgcug ggacuucaac aagaaccggg aggaguccga ccugcaccac     2160

gccguggacg cggccaucgu ggccugcacc agcccguccg acaucgcccg cgucaccgcc     2220

uucuaccagc ggcgcgagca gugcaaggag agcgcgaaga aggccgagcc ccacuucccc     2280

cagcccuggc cgcacuucgc cgacgagcug cgggcccgcc uguccaagaa ccccaaggag     2340

agcaucaagg cccucaaccu gggcaacuac gacgaccaga agcuggaguc ccuccagccc     2400

guguucguga gccggaugcc gaagcgcucc gucaccggcg cggcccacca ggagacgcug     2460

cggcgcuaca ucgggaucga cgagcggagc ggcaagaucc agaccguggu gaagaccaag     2520

cuguccgaga ucaagcucga cgagaccggg cacuucccca uguacggcaa ggagagcgac     2580

ccgcccgcca gcgccuga                                                   2598


<210>  1396
<211>  2886
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1396
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacaa cggcgacgag      420

gagaagggca agauccugaa ggccaucaag cagaacgagg agaagcuggu gaacuaccag      480

agcguggggg aguaccucua caaggaguac uuccagaagu ucaaggagaa cuccaaggag      540

uucaucaacg uccggaacaa gaaggagagc uacgagcgcu gcaucacgca guccuuccug      600

aaguacgagc ugaagcucau cuuccagaag cagcgggagu ucggcuucag cuucuccaag      660

aaguucgagg aggaggugcu gagcguggcg uucuacaagc gcgcccugaa ggacuucucc      720

caccucgucg ggaacugcag cuucuucucc gacgagaagc gggcccccaa gaacagcccc      780

cuggccuuca uguucguggc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggca uccucuacac caaggacgac cugaacgcgc ugcucaacga ggugcugaag      900

aacgggacgc ugaccuacaa gcagaccaag aagcuccugg gccugagcga cgacuacgag      960

uucaagggcg agaaggggac cuacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucggcg agcacaaccu gucccaggac gaccugaacg agaucgccaa ggacaucacg     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaccagaac     1140

cagaucgaca gccuguccaa gcuggaguuc aaggaccacc ucaacaucgg guucaaggcg     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga aggucgccau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu gaccaacccc gugguccugc gggccaucaa ggaguaccgc     1380

aaggugcuga acgcgcuccu gaagaaguac ggcaaggugc acaagaucaa caucgagcug     1440

gcccgcgagg ucgggaagaa ccacagccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgcggagcuc gagugcgaga agcugggccu gaagaucaac     1560

uccaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacagc     1620

ggggagaaga ucaagcucuc cgaccugcag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuaca gccgguccuu cgacgacagc uacaugaaca aggugcucgu guucacgaag     1740

cagaaccagg agaagcugaa caagaccccg uucgaggccu ucggcaacga caacgucaag     1800

uggcagaaga ucgaggugcu ggccaagaac cuccccgaga agaagcagaa gcgcauccug     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacc     1920

cgcuacaucg cccggcucgu gcugaacuac accaaggacu accuggacuu ccucccccug     1980

uccgacgacg agaacacgaa gcugaagggg agcaaggucc acguggaggc gaaguccggc     2040

augcucacca gcgcccugcg ccacaccugg ggcuucucca ccaaggaccg gaacaaccac     2100

cuccaccacg ccaucgacgc cgccaucauc gcguacgcca acaacagcau cgugaaggac     2160

uucuccgacu ucaagaagga gcaggagagc aacuccgccg agcuguacgc caagaagauc     2220

agcgagcugg acuacaagaa caagcgcaag uucuucgagc ccuucagcgg guuccggcag     2280

aagguccugg acaagaucga cgagaucuuc guguccaagc cggagcgcaa gaagcccagc     2340

ggcgcccucc acgaggagac guuccggaag gaggaggagu ucuaccaguc cuacgggggc     2400

aaggaggggg uccugaaggc gcuggagcuc ggcaagaucc gcaaggugaa cggcaagauc     2460

gugaagaacg gggacauguu ccgggucgac aucuucaagc acaagaagac caacaaguuc     2520

uacgccgugc ccaucuacac cauggacuuc gcccugaagg ugcugcccaa caaggccguc     2580

gcgcgcagca agaagggcga gaucaaggac uggauccuca uggacgagaa cuacgaguuc     2640

ugcuucuccc uguacaagga cagccugauc cucauccaga ccaaggacau gcaggagccg     2700

gagcuggugu acuucaacgc cuucacgucc agcaccgugu cccugaucgu cagcaagcac     2760

gacaacaagu ucgagacccu cuccaagaac cugaaggugu ucgagaagua caucgugagc     2820

gcccuggggg aggucaccaa ggccgaguuc cggcagcgcg aggacuucaa gaacgagcuc     2880

cgguga                                                                2886


<210>  1397
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1397
auggccaaga uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgc aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcggcgcauc gcccggagcg cccgcaagcg gcucgcgcgc      180

cggaaggccc gccugaacca ccugaagcac cucaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagauccug gccaaggcgu acaagggcag ccucaucucc      300

cccuacgagc ugcgguuccg cgcccugaac gagcuccuga gcaagcagga cuucgcccgg      360

gucauccugc acaucgccaa gcgccggggg uacgacgaca ucaagaacaa cggcgacgag      420

gagaagggca agauccucaa ggccaucaag cagaacgagg agaagcuggc gaacuaccag      480

uccguggggg aguaccugua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucaucaacg ugcgcaacaa gaaggagucc uacgagcggu gcaucgccca gagcuuccuc      600

aaggacgagc ugaagcugau cuucaagaag cagcgcgagu ucggcuucuc cuucagcaag      660

aaguucgagg aggagguccu cuccguggcc uucuacaagc gggcccugaa ggacuucagc      720

caccuggugg ggaacugcuc cuucuucacg gacgagaagc gcgcccccaa gaacagcccc      780

cucgcguuca uguucgucgc ccugacccgg aucaucaacc ugcucaacaa ccugaagaac      840

acccuguaca ccaaggacga ccucaacgcc cugcugaacg aggugcucaa gaacggcacg      900

cugaccuaca agcagaccaa gaagcugcuc gggcuguccg acgacuacga guucaagggc      960

gagaagggca acuacuucau cgaguucaag aaguacaagg aguucaucaa ggcccugggg     1020

gagcacagcc ucagccagga cgaccugaac gagaucgcca aggacaucac ccugaucaag     1080

gacgagauca agcucaagaa ggcgcuggcc aaguacgacc ugaacgagaa ccagaucgac     1140

aaccucucca agcuggaguu caaggaccac cugaacauca gcuucaaggc ccucaagcug     1200

aucacgccgc ugaugcugga gggcaagaag uacgacgagg ccugcaacga gcucacccug     1260

aagguggcca ucaacgagga caagaaggac uuccugcccg cguucaacga gaccuacuac     1320

aaggacgagg ucaccaaccc cguggugcuc cgcgccauca aggaguaccg gaagguccug     1380

aacgcccugc ucaagaagua cgggaaggug cacaagauca acaucgagcu ggcccgcgag     1440

gugggcaaga accacuccca gcgcgcgaag aucgagaagg agcagaacga gaacuacaag     1500

gccaagaagg acgccgagcu ggagugcgag aagcucggcc ugaagaucaa cagcaagaac     1560

auccugaagc uccggcuguu caaggagcag aaggaguucu gcgucuacuc cggggagaag     1620

aucaagcuga gcgaccucca ggacgagaag augcuggaga ucgaccacau cuaccccuac     1680

ucccgcagcu ucgacgacuc cuacaugaac aaggugcugg uguucacgaa gcagaaccag     1740

gagaagcuca accagacccc guucgaggcc uucggcaacg acagcgccaa guggcagaag     1800

aucgaggucc uggcgaagaa ccugcccacc aagaagcaga agcggauccu cgacaagaac     1860

uacaaggaca aggagcagaa ggacuucaag gaccgcaacc ugaacgacac ccgguacauc     1920

gcccgccugg ugcucaacua cacgaaggac uaccuggacu uccugccccu cuccgacgac     1980

gagaacacca agcugaacga cacccagaag gggagcaagg ugcacgucga ggccaagucc     2040

ggcaugcuga ccagcgcccu ccggcacacg uggggguucu ccaccaagga ccgcaacaac     2100

caccuccacc acgccaucga cgcggugauc aucgccuacg ccaacaacag caucgugaag     2160

gccuucuccg acuucaagaa ggagcaggag agcaacaccg ccgagcugua cgcgaagaag     2220

aucuccgagc uggacuacaa gaacaagcgg aaguucuucg agcccuucag cggcuuccgc     2280

cagaaggucc uggacaagau cgacgagauc uucgugagca agccggagcg gaagaagccc     2340

uccggcgccc ugcacgagga gaccuuccgc aaggaggagg aguucuacca gagcuacggg     2400

ggcaaggagg ggguccucaa ggcccuggag cugggcaaga uccggaaggu gaacgggaag     2460

aucgugaaga acggcgacau guuccgcguc gacaucuuca agcacaagaa gacgaacaag     2520

uucuacgccg ugcccaucua caccauggac uucgcgcuca aggugcugcc caacaaggcc     2580

gucgcccggu ccaagaaggg cgagaucaag gacuggaucc ugauggacga gaacuacgag     2640

uucugcuuca gccucuacaa ggacucccug auccugaucc agaccaagga caugcaggag     2700

ccggaguucg uguacuacaa cgccuucacc agcuccacgg ugagccucau cgucuccaag     2760

cacgacaaca aguucgagac ccugagcaag aaccagaaga uccuguucaa gaacgccaac     2820

gagaaggagg ugaucgcgaa guccaucggg auccagaacc ucaagguguu cgagaaguac     2880

aucgucagcg cccugggcga ggugaccaag gccgaguucc gccagcggga ggaguucaag     2940

aacgagcugc gcuga                                                      2955


<210>  1398
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1398
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaaccg ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgguuccg cgcccucaac gagcugcugu ccaagcagga cuucgcccgg      360

gucauccucc acaucgccaa gcgccggggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcgaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcgcaacaa gaaggagucc uacgagcggu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgcgagu ucggguucuc cuucagcaag      660

aaguucgagg aggagguccu guccguggcc uucuacaagc gggcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gcgcgcccaa gaacagcccc      780

cuggccuuca uguucgucgc ccugacccgg aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga guuccugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg agcacaaccu gucccaggac gaccugaacg agaucgcgaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaccagaac     1140

cagaucgaca gccuguccaa gcuggaguuc aaggaccacc ucaacaucag cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggcgau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu gacgaacccc gucgugcugc gcgccaucaa ggaguaccgc     1380

aaggugcuga acgcccuccu gaagaaguac gggaaggucc acaagaucaa caucgagcug     1440

gcgcgggagg ugggcaagaa cuacucccag cgcgccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

agcaagaaca uccucaagcu gcggcuguuc aaggagcaga aggaguucug cgccuacucc     1620

ggggagaaga ucaagaucag cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuacu cccgcagcuu cgacgacucc uacaugaaca aggugcuggu cuucaccaag     1740

cagaaccagg agaagcucaa ccagaccccg uucgaggcgu ucggcaacga cagcgccaag     1800

uggcagaaga ucgaggugcu ggccaagaac cugcccacca agaagcagaa gcggauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accgcaaccu gaacgacacg     1920

cgguacaucg cccgccuggu gcucaacuac accaaggacu accuggacuu ccugccccuc     1980

uccgacgacg agaacaccaa gcugaacgac acccacaagg ggagcaaggu ccacguggag     2040

gccaaguccg gcaugcugac gagcgcgcuc cggcacaccu ggggguucuc caccaaggac     2100

cgcaacaacc accuccggca cgccaucgac gccgugauca ucgccuacgc caacaacagc     2160

aucgucaagg cguucuccga cuucaagaag gagcaggaga gcaacagcgc cgagcuguac     2220

gccaagaaga ucuccgagcu ggacuacaag aacaagcgca aguucuucga gcccuucagc     2280

ggcuuccggc agaaggugcu ggacaagauc gacgagaucu ucguguccaa gccggagcgc     2340

aagaagccca gcggcgcccu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

uccuacgggg gcaaggaggg gguccucaag gcgcuggagc ugggcaagau ccgcaaggug     2460

aacgggaaga ucgucaagaa cggcgacaug uuccgggugg acaucuucaa gcacaagaag     2520

acgaacaagu ucuacgccgu gcccaucuac accauggacu ucgcccucaa gguccugccc     2580

aacaaggccg uggcccgcag caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucuc ccucuacaag gacagccuga uccugaucca gaccaaggac     2700

augcaggagc cggaguucgu guacuacaac gcguucaccu ccagcacggu cucccucauc     2760

gugagcaagc acgacaacaa guucgagacc cuguccaaga accagaagau ccuguucaag     2820

aacgccaacg agaaggaggu gaucgccaag agcaucggga uccagaaccu caaggucuuc     2880

gagaaguaca ucguguccgc ccugggcgag gugaccaagg ccgaguuccg gcagcgcgag     2940

gacuucaaga aguga                                                      2955


<210>  1399
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1399
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggucgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcccgg      180

cgcaaggcgc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggccu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcgcucaac gagcugcugu ccaagcagga cuucgcccgc      360

gugauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggccaucaag cagaacgagg agaagcuggc gaacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg uccggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggaggugcu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgucg gcaacugcuc cuucuucacc gacgagaagc gggcccccaa gaacagcccc      780

cuggcguuca uguucgugac ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

acggagggga uccucuacac caaggacgac cugaacgccc ugcucaacga ggugcugaag      900

aacggcaccc ugaccuacaa gcagacgaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac cuacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg agcacaaccu gucccaggac gaccugaacg agaucgccaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcga aguacgaccu caaccagaac     1140

cagaucgaca gccuguccaa gcuggaguuc aaggaccacc ucaacaucag cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga aggucgccau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

acguacuaca aggacgaggu gaccaacccc gugguccugc gggcgaucaa ggaguaccgc     1380

aaggugcuga acgcccuccu gaagaaguac gggaaggugc acaagaucaa caucgagcug     1440

gcccgcgagg ucggcaagaa ccacucccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg cgaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

agcaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacucc     1620

ggggagaaga ucaagaucag cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuacu cccggagcuu cgacgacucc uacaugaaca aggugcuggu guucaccaag     1740

cagaaccagg agaagcucaa caagaccccg uucgaggccu ucggcaacga cagcgccaag     1800

uggcagaaga ucgagguccu ggcgaagaac cugcccacga agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacc     1920

cgcuacaucg cccggcuggu gcucaacuac accaaggacu accuggacuu ccugccccuc     1980

uccgacgacg agaacaccaa gcugaacgac acgcagaagg ggagcaaggu gcacgucgag     2040

gccaaguccg gcaugcugac cagcgcccuc cgccacaccu ggggguucuc caccaaggac     2100

cggaacaacc accuccacca cgccaucgac gcggugauca ucgccuacgc caacaacagc     2160

aucgugaagg ccuucuccga cuucaagaag gagcaggaga gcaacagcgc cgagcuguac     2220

gcgaagaaga ucuccgagcu ggacuacaag aacaagcgca aguucuucga gcccuucagc     2280

ggcuuccggc agaagguccu ggacaagauc gacgagaucu ucguguccaa gccggagcgc     2340

aagaagccca gcggcgcccu gcacgaggag acguuccgga aggaggagga guucuaccag     2400

uccuacgggg gcaaggaggg gguccucaag gcccuggagc ugggcaagau ccgcaaggug     2460

aacgggaaga ucgugaagaa cggcgacaug uuccgggucg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu gcccaucuac accauggacu ucgcgcucaa ggugcugccc     2580

aacaaggccg ucgcccgcag caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucuc ccucuacaag gacagccuga uccugaucca gaccaaggac     2700

augcaggagc cggaguucgu guacuacaac gccuucacgu ccagcaccgu gucccucauc     2760

gucagcaagc acgacaacaa guucgagacc cuguccaaga accagaagau ccuguucaag     2820

aacgccaacg agaaggaggu gaucgccaag agcaucggga uccagaaccu caagguguuc     2880

gagaaguaca ucgucuccgc gcugggcgag gugaccaagg ccgaguuccg gcagcgcgag     2940

gacuucaaga aguga                                                      2955


<210>  1400
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1400
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcgaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggagguccu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gggcgcccaa gaacagcccc      780

cuggccuuca uguucgucgc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga ggugcugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg accacucccu gagccaggac gaccugaacg agaucgcgaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaccagaac     1140

cagaucgacu cccugagcaa gcuggaguuc aaggaccacc ucaacaucuc cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggcgau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu cacgaacccc guggugcugc gggccaucaa ggaguaccgc     1380

aagauccuga acgcccuccu gaagaaguac gggaaggucc acaagaucaa caucgagcug     1440

gcgcgcgagg ugggcaagaa ccacagccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagauc gagugcgaga agcucggccu gaagaucaac     1560

uccaagaaca uccugaagcu ccgccuguuc aaggagcaga aggaguucug cgccuacagc     1620

ggggagaaga ucaagcuguc cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuaca gccgguccuu cgacgacagc uacaugaaca aggugcuggu cuucaccaag     1740

cagaaccagg agaagcucaa caagaccccg uucgaggcgu ucggcaacga cuccaccaag     1800

uggcagaaga ucgaggugcu ggccaagaac cugcccgaga agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu gcucaacuac accaaggacu accuggacuu ccugccccuc     1980

agcgacgacg agaacaccaa gcugaacgac acccagaagg gguccaaggu ccacguggag     2040

gccaagagcg gcaugcugac guccgcccuc cgccacaccu ggggguucag cgcgaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgccauca ucgccuacgc gaacaacucc     2160

aucgugaagg ccuucagcga cuucaagaag gagcaggaga gcaacuccgu cgagcuguac     2220

gccaagaaga ucagcgagcu ggacuacaag aacaagcgca aguucuucga gcccuucucc     2280

ggcuuccggc agaaggugcu ggacaagauc gacgagaucu ucgugagcaa gccggagcgc     2340

aagaagcccu ccggcgcccu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

agcuacgggg gcaaggaggg gguccucaag gcgcuggagc ugggcaagau ccgcaaggug     2460

aacgggaaga ucgucaagaa cggcgacaug uuccgggugg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu gcccaucuac acgauggacu ucgcccucaa gguccugccc     2580

aacaaggccg uggcccgcuc caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucag ccucuacaag gacucccuga uccugaucca gaccaaggac     2700

augcaggagc cggagcucgu guacuucaac gcguucacca gcuccaccgu cagccugauc     2760

guguccaagc acgacaacaa guucgagacg cugagcaaga accagaagau ccucuucaag     2820

aacgccaacg agaaggaggu gaucgccaag uccaucggga uccagaaccu gaaggucuuc     2880

gagaaguaca ucgugagcgc ccugggcgag gugaccaagg ccgaguuccg gcagcgcgag     2940

gacuucaaga aguga                                                      2955


<210>  1401
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1401
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggucgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcccgg      180

cgcaaggcgc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggccu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcgcucaac gagcugcugu ccaagcagga cuucgcccgc      360

gugauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggccaucaag cagaacgagg agaagcuggc gaacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg uccggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggaggugcu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgucg gcaacugcuc cuucuucacc gacgagaagc gggcccccaa gaacagcccc      780

cuggcguuca uguucguggc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga ggugcugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg agcacaaccu gucccaggac gaccugaacg agaucgccaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcgcuggcca aguacgaccu caaccagaac     1140

cagaucgaca gccuguccaa gcuggaguuc aaggaccacc ucaacaucag cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga aggucgccau caacgaggac aagaaggacu uccuccccgc guucaacgag     1320

accuacuaca aggacgaggu gacgaacccc gugguccugc gggccaucaa ggaguaccgc     1380

aaggugcuga acgcccuccu gaagaaguac gggaaggugc acaagaucaa caucgagcug     1440

gcccgcgagg ucggcaagaa ccacucccag cgggcgaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

agcaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacucc     1620

ggggagaaga ucaagaucag cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuacu cccggagcuu cgacgacucc uacaugaaca aggugcuggu guucaccaag     1740

cagaaccagg agaagcucaa caagaccccg uucgaggccu ucggcaacga cagcgcgaag     1800

uggcagaaga ucgagguccu ggccaagaac cugcccacca agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu gcucaacuac accaaggacu accuggacuu ccugccccuc     1980

uccgacgacg agaacaccaa gcugaacgac acccagaagg ggagcaaggu gcacgucgag     2040

gccaaguccg gcaugcugac gagcgcccuc cgccacaccu ggggguucuc caccaaggac     2100

cggaacaacc accuccacca cgcgaucgac gccgugauca ucgccuacgc caacaacagc     2160

aucgugaagg ccuucuccga cuucaagaag gagcaggaga gcaacagcgc ggagcuguac     2220

gccaagaaga ucuccgagcu ggacuacaag aacaagcgca aguucuucga gcccuucagc     2280

ggcuuccggc agaagguccu ggacaagauc gacgagaucu ucguguccaa gccggagcgc     2340

aagaagccca gcggcgcccu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

uccuacgggg gcaaggaggg gguccucaag gcccuggagc ugggcaagau ccgcaaggug     2460

aacgggaaga ucgugaagaa cggcgacaug uuccgggucg acaucuucaa gcacaagaag     2520

acgaacaagu ucuacgcggu gcccaucuac accauggacu ucgcccucaa ggugcugccc     2580

aacaaggccg ucgcccgcag caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucuc ccucuacaag gacagccuga uccugaucca gaccaaggac     2700

augcaggagc cggaguucgu guacuacaac gccuucaccu ccagcacggu gucccucauc     2760

gucagcaagc acgacaacaa guucgagacc cuguccaaga accagaagau ccuguucaag     2820

aacgcgaacg agaaggaggu gaucgccaag agcaucggga uccagaaccu caagguguuc     2880

gagaaguaca ucgucuccgc ccugggcgag gugaccaagg ccgaguuccg gcagcgcgag     2940

gacuucaaga aguga                                                      2955


<210>  1402
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1402
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcgaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggagguccu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gggcgcccaa gaacagcccc      780

cuggccuuca uguucgucgc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga ggugcugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg accacucccu gagccaggac gaccugaacg agaucgcgaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaccagaac     1140

cagaucgacu cccugagcaa gcuggaguuc aaggaccacc ucaacaucuc cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggcgau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu cacgaacccc guggugcugc gggccaucaa ggaguaccgc     1380

aagguccuga acgcccuccu gaagaaguac gggaaggugc acaagaucaa caucgagcug     1440

gcgcgcgagg ugggcaagaa cuacagccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

uccaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacagc     1620

ggggagaaga ucaagaucuc cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuaca gccgguccuu cgacgacuuc uacaugaaca agguccuggu guucaccaag     1740

cagaaccagg agaagcucaa ccagaccccg uucgaggcgu ucggcaacga cagcgccaag     1800

uggcagaaga ucgaggugcu ggccaagaac cugcccacca agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu ccucaacuac accaaggacu accuggacuu ccugccccuc     1980

uccgacgacg agaacaccaa gcugaacgac acccagaagg ggagcaaggu gcacguggag     2040

gccaaguccg gcaugcugac gagcgcgcuc cgccacaccu ggggguucuc cgccaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgucauca ucgccuacgc gaacaacagc     2160

aucgugaagg ccuucuccga cuucaagaag gagcaggaga gcaacagcgc cgagcuguac     2220

gccaagaaga ucuccgagcu ggacuacaag aacaagcgca aguucuucga gcccuucagc     2280

ggcuuccggc agaaggugcu ggacaagauc gacgagaucu ucgucuccaa gccggagcgc     2340

aagaagccca gcggcgcgcu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

uccuacgggg gcaaggaggg ggugcucaag gcccuggagc ugggcaagau ccgcaagguc     2460

aacgggaaga ucgugaagaa cggcgacaug uuccgggugg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu ccccaucuac acgauggacu ucgcccucaa ggugcugccc     2580

aacaaggccg uggcgcgcag caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucuc ccucuacaag gacagccuga uccugaucca gaccaaggac     2700

augcaggagc cggaguucgu cuacuacaac gccuucaccu ccagcaccgu gucccucauc     2760

gugagcaagc acgacaacaa guucgagacg cuguccaaga accagaagau ccuguucaag     2820

aacgccaacg agaaggaggu caucgccaag agcaucggga uccagaaccu caagguguuc     2880

gagaaguaca ucguguccgc ccugggcgag gucaccaagg cggaguuccg gcagcgcgag     2940

gacuucaaga aguga                                                      2955


<210>  1403
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1403
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcgaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggagguccu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gggcgcccaa gaacagcccc      780

cuggccuuca uguucgucgc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga guuccugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg agcacaaccu gucccaggac gaccugaacg agaucgcgaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaccagaac     1140

cagaucgaca gccuguccaa gcuggaguuc aaggaccacc ucaacaucag cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agaucgcgau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu gacgaacccc gugguccugc gggccaucaa ggaguaccgc     1380

aaggugcuga acgcccuccu gaagaaguac gggaaggugc acaagaucaa caucgagcug     1440

gcgcgcgagg ucggcaagaa ccacucccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

agcaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacucc     1620

ggggagaaga ucaagaucag cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuacu cccggagcuu cgacgacucc uacaugaaca aggugcuggu guucaccaag     1740

cagaaccagg agaagcucaa ccagaccccg uucgaggcgu ucggcaacga cagcgccaag     1800

uggcagaaga ucgagguccu ggccaagaac cugcccacca agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag aacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu gcucaacuac accaaggacu accuggacuu ccugccccuc     1980

uccgacgacg agaacaccaa gcugaacgac acccagaagg ggagcaaggu gcacgucgag     2040

gccaaguccg gcaugcugac gagcgcgcuc cgccacaccu ggggguucuc cgccaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgugauca ucgccuacgc gaacaacagc     2160

aucgugaagg ccuucuccga cuucaagaag gagcaggaga gcaacagcgc cgagcuguac     2220

gccaagaaga ucuccgagcu ggacuacaag aacaagcgca aguucuucga gcccuucagc     2280

ggcuuccggc agaagguccu ggacaagauc gacgagaucu ucguguccaa gccggagcgc     2340

aagaagccca gcggcgcgcu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

uccuacgggg gcaaggaggg ggugcucaag gcccuggagc ugggcaagau ccgcaagguc     2460

aacgggaaga ucgugaagaa cggcgacaug uuccgggucg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu gcccaucuac acgauggacu ucgcccucaa ggugcugccc     2580

aacaaggccg ucgcgcgcag caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucuc ccucuacaag gacagccuga uccugaucca gaccaaggac     2700

augcaggagc cggaguucgu guacuacaac gccuucaccu ccagcaccgu gucccucauc     2760

gucagcaagc acgacaacaa guucgagacg cuguccaaga accagaagau ccuguucaag     2820

aacgccaacg agaaggaggu gaucgccaag agcaucggga uccagaaccu caagguguuc     2880

gagaaguaca ucgucuccgc ccugggcgag gugaccaagg cggaguuccg gcagcgcgag     2940

gacuucaaga aguga                                                      2955


<210>  1404
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1404
auggugcgca uccuggccuu cgacaucggc aucagcucca ucgggugggc cuucagcgag       60

aacgacgagc ucaaggacug cggcguccgg aucuucacca agguggagaa ccccaagacc      120

ggggaguccc uggcgcugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcccgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcga acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggccu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcgcgc      360

gugauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggccaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgucggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgcgca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggaggugcu guccgucgcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gggcccccaa gaacagcccc      780

cuggccuuca uguucguggc gcugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga gguccugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg agcacaaccu gucccaggac gaccugaacg agaucgccaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcga aguacgaccu caaccagaac     1140

cagaucgaca gccuguccaa gcuggaguuc aaggaccacc ucaacaucag cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggccau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu gacgaacccc gucgugcugc gggcgaucaa ggaguaccgc     1380

aaggugcuga acgcccuccu gaagaaguac gggaaggucc acaagaucaa caucgagcug     1440

gcccgcgagg ugggcaagaa ccacucccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg cgaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

agcaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacucc     1620

ggggagaaga ucaagaucag cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuacu cccggagcuu cgacgacucc uacaugaaca aggugcuggu cuucaccaag     1740

cagaaccagg agaagcucaa caagaccccg uucgaggccu ucggcaacga cagcgccaag     1800

uggcagaaga ucgaggugcu ggcgaagaac cugcccacca agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu gcucaacuac accaaggacu accuggacuu ccugccccuc     1980

uccgacgacg agaacaccaa gcugaacgac acccagaagg ggagcaaggu ccacguggag     2040

gccaaguccg gcaugcugac gagcgcccuc cgccacaccu ggggguucuc caccaaggac     2100

cggaacaacc accuccacca cgccaucgac gcggugauca ucgccuacgc caacaacagc     2160

aucgucaagg ccuucuccga cuucaagaag gagcaggaga gcaacagcgc cgagcuguac     2220

gcgaagaaga ucuccgagcu ggacuacaag aacaagcgca aguucuucga gcccuucagc     2280

ggcuuccggc agaaggugcu ggacaagauc gacgagaucu ucgucuccaa gccggagcgc     2340

aagaagccca gcggcgcccu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

uccuacgggg gcaaggaggg ggugcucaag gcccuggagc ugggcaagau ccgcaaggug     2460

aacgggaaga ucgucaagaa cggcgacaug uuccgggugg acaucuucaa gcacaagaag     2520

acgaacaagu ucuacgccgu gcccaucuac accauggacu ucgcgcucaa gguccugccc     2580

aacaaggccg uggcccgcag caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucuc ccucuacaag gacagccuga uccugaucca gaccaaggac     2700

augcaggagc cggaguucgu guacuacaac gccuucaccu ccagcacggu cucccucauc     2760

gugagcaagc acgacaacaa guucgagacc cuguccaaga accagaagau ccuguucaag     2820

aacgccaacg agaaggaggu gaucgccaag agcaucggga uccagaaccu caaggucuuc     2880

gagaaguaca ucguguccgc gcugggcgag gugaccaagg ccgaguuccg gcagcgcgag     2940

gacuucaaga aguga                                                      2955


<210>  1405
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1405
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcgaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggagguccu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gggcgcccaa gaacagcccc      780

cuggccuuca uguucgucgc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga ggugcugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg accacucccu gagccaggac gaccugaacg agaucgcgaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaccagaac     1140

cagaucgacu cccugagcaa gcuggaguuc aaggaccacc ucaacaucuc cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggcgau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu cacgaacccc guggugcugc gggccaucaa ggaguaccgc     1380

aagguccuga acgcccuccu gaagaaguac gggaaggugc acaagaucaa caucgagcug     1440

gcgcgcgagg ugggcaagaa cuacagccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

uccaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacagc     1620

ggggagaaga ucaagaucuc cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuaca gccgguccuu cgacgacagc uacaugaaca agguccuggu guucaccaag     1740

cagaaccagg agaagcucaa ccagaccccg uucgaggcgu ucggcaacga cuccgccaag     1800

uggcagaaga ucgaggugcu ggccaagaac cugcccacca agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu ccucaacuac accaaggacu accuggacuu ccugccccuc     1980

agcgacgacg agaacaccaa gcugaacgac acccagaagg gguccaaggu gcacguggag     2040

gccaagagcg gcaugcugac guccgcgcuc cgccacaccu ggggguucag cgccaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgucauca ucgccuacgc gaacaacucc     2160

aucgugaagg ccuucagcga cuucaagaag gagcaggaga gcaacuccgc cgagcuguac     2220

gccaagaaga ucagcgagcu ggacuacaag aacaagcgca aguucuucga gcccuucucc     2280

ggcuuccggc agaaggugcu ggacaagauc gacgagaucu ucgucagcaa gccggagcgc     2340

aagaagcccu ccggcgcgcu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

agcuacgggg gcaaggaggg ggugcucaag gcccuggagc ugggcaagau ccgcaagguc     2460

aacgggaaga ucgugaagaa cggcgacaug uuccgggugg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu ccccaucuac acgauggacu ucgcccucaa ggugcugccc     2580

aacaaggccg uggcgcgcuc caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucag ccucuacaag gacucccuga uccugaucca gaccaaggac     2700

augcaggagc cggaguucgu cuacuacaac gccuucacca gcuccaccgu gagccucauc     2760

guguccaagc acgacaacaa guucgagacg cugagcaaga accagaagau ccuguucaag     2820

aacgccaacg agaaggaggu caucgccaag uccaucggga uccagaaccu caagguguuc     2880

gagaaguaca ucgugagcgc ccugggcgag gucaccaagg cggaguuccg gcagcgcgag     2940

gacuucaaga aguga                                                      2955


<210>  1406
<211>  2958
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1406
auggccaaga uccuggccuu cgacaucggc aucagcucca ucgggugggc guucguggag       60

aacgacgagc ucaaggacug cggcguccgc aucuucacca aggccgagaa ccccaagacc      120

ggggagagcc uggcccugcc gcggcgccuc gcccgguccg cccgcaagcg gcuggcgcgc      180

cggaaggccc gccugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc agagcuucga cgagucccuc gccaaggcgu acaagggcac gcugaucagc      300

cccuacgagc ugcgguucaa ggcccucaac gagcugcugu ccaagcagga ccucgcccgc      360

gugauccugc acaucgccaa gcggcgcggg uacgacaaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcggugaag gagaacgagg agaagcucgc caacuacaag      480

uccgucggcg aguaccugua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucaucaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ucaagcugau cuuccagaag cagcgggagu ucggguucaa cuucuccaag      660

aaguucgagg aggaggugcu gagcgucgcc uucuacaagc gcgcccucaa ggacuucucc      720

caccuggugg gcaacugcag cuucuucgug gacgagaagc gggcgcccaa gaacagcccc      780

cuggccuucg aguucgucgc ccucacccgc aucgugaacc ugcugaacaa ccucaacaag      840

aacaacagcu acaucaagga cgaccugaac gcccugcucu ccgaggugcu gaagaacggg      900

acccugaccu acaagcagac gaagaagauc cucggccuga gcgacgacua cgaguucaag      960

gggcagaagg gcaacuacuu caucgaguuc aagaaguaca aggaguucau caaggcccug     1020

ugcgagcaca accucaacca ggacgaccug aacgagaucg cgaaggacau cacccugguc     1080

aaggacgaga ccaagcucaa gaaggcccug gccaaguacg accugaacca gaaccagauc     1140

gagaaccuca gcaagaucga guucaaggac caccugaaca ucuccuucaa ggcccugaag     1200

cucaucgugc cgcugaugcg ggagggcaag aaguacgacg aggugugcaa cgagcugaac     1260

cugaagaccg ccaucaacga ggacaagaag gaguuccucc ccgcguucaa cgagacguac     1320

uacaaggacg aggucaacaa ccccguggug cugcgcgcca ucaaggagua ccggaagguc     1380

cugaacagcc uccugaagaa guacgggaag gugcacaaga ucaacaucga gcuggcccgc     1440

gaggugggca agaaccaccg cgagcggucc aagaucgaga aggagcagaa cgagaacuuc     1500

aaggccaaga aggacgcgga gcucgagugc gagaagcugg ggcugaagau caacagcaag     1560

aacauccuca agcugcgccu guucaaggag cagaaggagu ucugcgucua cuccggcgag     1620

aagaucaaga ucagcgaccu ccaggacgag aagaugcugg agaucgacca caucuacccc     1680

uacucccgga gcuucgacga cuccuacaug aacaaggugc ugguguucac caagcagaac     1740

caggagaagc ucaacaagac cccguucgag gccuucggca aggacgacgu caaguggcag     1800

aagaucgaga cccuggccaa gaaccugccc gccaagaagc agaagcgcau ccucgacaag     1860

aacuacaagg acaaggagca gaaggacuuc aaggaccgga accugaacga cacgcgcuac     1920

aucgcccggc uggugcucaa cuacaccaag gacuaccugg aguuccugcc ccucagcgac     1980

gacgagaaca ucaagcugaa cgacacccag aaggggucca agguccacgu ggaggcgaag     2040

uccggcaugc ugaccuccgu gcuccgccac acgugggggu ucagcaccaa ggaccggaac     2100

aaccaccucc accacgccau cgacgccguc aucaucgccu acgccaacgc guccguggug     2160

aaggccuuca gcgacuucaa gaaggagcag gagaacuaca ccgccgagcu guacgccaag     2220

aagaucaacg agcuggacuu caagaacaag cgcaaguucu ucgagcccuu cuccaacuuc     2280

cggcagaagg uccuggagaa gaucgacgag aucuucguga gcaagccgga gcgcaagaag     2340

cccuccggcg cccugcacga ggagaccuuc uacaaggagg aggaguucua caaggaguac     2400

gggggcaagg agggcgugcu caaggcgcug gagcugggga agauccggaa ggucaacggc     2460

aagaucguga gcaacgccaa cauguuccgc guggacaucu ucaaguacaa gaagacgaac     2520

aaguucuacg ccguccccau cuacaccaug gacuucgccc ucgggauccu gcccaacaag     2580

gcgguggugg gcgggaagga caagcagggc aucaucaagg acuggcugcu cauggacgag     2640

aacuacgagu ucugcuucag ccuguacaag gacucccuga uccuccugca gaccaaggac     2700

augcaggagc cggagcuggu cuacuucaag agcuucaagu ccagcaccgc cggccucgug     2760

guguccaagc acgacaacaa guucgagaac cugaacgaga accagaagaa gcuguucaag     2820

agcgccaacg agaagaaggu cguggccgag gggaucggca uccagaaccu caagguguuc     2880

gagaaguaca ucgucuccgc ccugggggag aucacgaagg cggagaucaa ggagcgggag     2940

aacuucaaga agaaguga                                                   2958


<210>  1407
<211>  2961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1407
augcccagcg acccggccga ggccaagccc cuggagcucc uggaccccua cgcgcugcgc       60

gcccagggcc ucgaccaggc ccugccccug acccacuucg ggcgggcccu cuuccaccug      120

aaccagcgcc ggggcuucaa guccaaccgc aagaccgacc gcggggacaa cgagaacggc      180

aagaucaagg acgccagcca gcggcuggac ugggcgaugc acgaggccaa ggcccggacg      240

uacggggagu uccuccaccu ccgccggcag aaggccccgg acccccgcca ggugcccacc      300

guccggaccc gccuguccgu gggcaagcgg ggcgggcccg acgcgaagga ggaggccggc      360

uacgacuucu acccggaccg ccggcaccug gaggaggagu uccacgagcu guggagcgcc      420

caggcccagu uccaccccga gcugacccag gaccuccgcg accugguguu cgagaagauc      480

uucuaccagc ggccccugaa ggagcccaag gucgggcucu gccuguucac guccgaggag      540

cgccuggcca aggcgcaccc gcucacccag cggcgcgugc uguacgagac cgugaaccag      600

cugcggauca ccgccgacgg ccgcagcguc cgcccccucu cccgggagca gcgcgaccag      660

aucauccugg cccuggacac gaagaagccc accaagagcc ucaacuccau gguggugacc      720

cugcaggccc uggggaaggu ccucaagcug cgggagggcg agcgcuucac ccuggagagc      780

ggcgugcggg aggccaucgc gugcgacccc gugcgcgccg ccaugcucgc cacguucggg      840

ccgcgguggu ccgcccugga cuggcccgag caguggcagg ucaucagccg cgugcggaag      900

cugcaggacg acaaggacuu cgcggagcuc guggaguggc ugaucgagcu guacggcguc      960

gacaaggagg ccgccgugaa gaucgccaac acccaccucc ccgaggggua cggccgccug     1020

ggccugaccg ccaccacgcg gauccucgac cgccugaagu ccaacguguu caccuacgcg     1080

caggccgucg ccgccugcgg guggcaccac agcgaccagc ggaccggcga gugccuggac     1140

cgccuccccu acuacggggc ggugcuggac cggcacguga ucccgggcac cuacgacgag     1200

aacgacgacg acaucacgcg cuucgggcgg aucaccaacc ccaccgucca caucggccug     1260

aaccagcucc gccggcuggu gaacaagauc aucgaggugu acggcaagcc caaccagauc     1320

gucguggagc uggcccgcga gcugaagcag uccgagcagc agaagaacga cgccaucaag     1380

cucauccgcg acaccacgac cgccgccaag cagcggagcg agaagcugga ggagcugggg     1440

aucaccgaca acggccgcaa ccggaugcuc cugcgccugu gggaggaccu caaccccgac     1500

gacgcgaugc ggcgcuucug cccguacacc gggcagcgga ucuccgccgc caugaucuuc     1560

gacggcagcu gcgacgucga ccacauccug cccuacuccc gcacgcugga cgacggguuc     1620

gccaaccgga cccucugccu gcgcgaggcc aaccgggaga agcgcaacca gacccccugg     1680

gaggcguggg gcggcacccc caacugggag gccaucgaga cgaaccugaa gaaccucccg     1740

gagaacaagc gguggcgcuu cgcccccgac gccauggccc gguucgaggg ggagaacgac     1800

uuccuggacc gcgcgcuggu ggacacccag uaccucgccc ggaucagccg caccuaccug     1860

gacacccugu ucacggaggg cgggcacgug ugggucgugc ccggccggcu caccgagaug     1920

cugcgccggc acugggggcu gaacagccuc cugaccgaca aggaccgcgg cgccgugaag     1980

uccaagaacc gcaccgacca ccggcaccac agcaucgacg ccgcggucgu ggccgccacc     2040

gaccgcuccc ugcucaaccg gaucagccgc gccgccggcc agggggaggu ggcgggccag     2100

uccgccgagc ugaucgcccg ggagacgccg ccgcccuggg agggguuccg caacgaccuc     2160

aagagccagc ugggccggau caucgucucc caccgcgccg accacggccg gaucgaccgc     2220

caggcccgga agcaggggcg cgacagcacc gcgggccagc ugcaccagga gaccgccuac     2280

uccaucgugg acgacacccg gguggccagc cgcaucgacu uccuguccgu caagcccgcc     2340

cagcuccugg accagcccgg gcggagcggc caggugcgcg accugcagcu ccggaaggcc     2400

cugcgcgugg cgacggggga ccugaccggc aaggacuacg agaaggcccu ccgggacuuc     2460

gccgccaagc ccggccccua caaggggauc cgccgcgucc ggaucaucaa gccccugcag     2520

gagcaggcgc gcgugccggu gcccguccug gaccccauca aggccuacca gggcgggucc     2580

aaccacgugu ucgagaucug gcaccucccc gacggcaagg uggagccgca ggucaucacc     2640

agcuucgagg cccacgcccu ggagguggag aagcggcccc accccgccgc gaagcgccug     2700

cuccgggugc acaaggggga cauggucgcc cugaagcgcg agggccggac cguggugggc     2760

cacguccagc agaugcacau caagaacggg cuguucaucg ugccccacaa cgaggccaac     2820

gccgacgccc gcaacuccga ccggacggac ccguucaagu ggauccagau cgcggcccag     2880

cccgccaucg ccgccggcau ccgccgggug gcggucgacg agaucgggcg ccuccgggac     2940

ggcggggcca agaccaucug a                                               2961


<210>  1408
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1408
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc agccgcuccg cccggaagcg ccuggcccgg      180

cgcaaggcgc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc agagcuucga cgagucccuc gccaaggccu acaagggcag ccugaucucc      300

cccuacgagc ugcgcuuccg ggcgcucaac gaguuccuga gcaagcagga cuucgcccgc      360

gucauccugc acaucgccaa gcggcgcggg uacgacgaca ucaagaacaa cggcgacgag      420

gagaagggcg agauccucaa ggccaucaag cagaacgagg agaagcuggu guccuaccag      480

agcguggggg aguaccugua caaggaguac uuccagaagu ucaaggagaa cuccaaggag      540

uucaucaacg uccggaacaa gaaggagagc uacgagcgcu gcaucgccca guccuuccuc      600

aaggacgagc ugaagcugau cuuccagaag cagcgggagu ucggcuucag cuucuccaag      660

aaguucgagg aggaggugcu cagcguggcg uucuacaagc gcgcccugaa ggacuucucc      720

caccuggucg ggaacugcag cuucuucacg gacgagaagc gggcccccaa gaacagcccc      780

cucgccuuca uguucguggc ccugacccgc aucaucaacc ugcucaacaa ccugaagaac      840

accgagggca uccuguacac caaggacgac cucaacacgc ugcugaacga ggugcucaag      900

aacgggaccc ugaccuacaa gcagaccaag aagcugcucg gccuguccaa cgacuacgag      960

uucaagggcg agaaggggac guacuucauc gaguuccaga aguacaagga guucgucaag     1020

gcgcugggcg agcacaaccu cagccaggac gaccugaacg agaucgccaa ggacaucacc     1080

cugaucaagg acgagaucaa gcucaagaag gcccuggcca aguacgaccu gaaccagaac     1140

cagaucgacu cccucagcaa gcuggaguuc aaggaccacc ugaacaucuc cuucaaggcc     1200

cucaagcuga ucaccccgcu gaugcuggag gggaagaagu acgacgaggc gugcaacgag     1260

cucaaccuga agguggccau caacgaggac aagaaggacu uccugcccgc cuucaacgag     1320

accuacuaca aggacgaggu gacgaacccc gucgugcucc gggccaucaa ggaguaccgc     1380

aaggugcuga acgcccugcu caagaaguac ggcaaggucc acaagaucaa caucgagcug     1440

gcgcgcgagg ugggcaagaa ccacagccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcug gagugcgaga agcucgggcu gaagaucaac     1560

uccaagaaca uccugaagcu ccgccuguuc aaggagcaga aggaguucug cguguacagc     1620

ggcgagaaga ucaagaucuc cgaccugcag gacgagaaga ugcucgagau cgaccacauc     1680

uaccccuaca gccgguccuu cgacgacagc uacaugaaca agguccuggu guucaccaag     1740

cagaaccagg agaagcugaa caagaccccg uucgagaccu ucgggaacga ccgcgugaag     1800

uggcagaaga ucgagguccu cgcgaagaac cugcccacga agaagcagaa gcggauccug     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accgcaaccu caacgacacc     1920

cgguacaucg cccgccuggu gcugaacuac accaaggacu accucgacuu ccugccccug     1980

uccgacgacg agaacaccaa gcucaacgac acgcagaagg gcagcaaggu gcacgucgag     2040

gccaaguccg ggaugcugac cagcacccug cggcacaccu ggggcuucuc cacgaaggac     2100

cgcgacaacc accuccacca cgccaucgac gccgcgauca ucgccuacgc caacaacagc     2160

aucgugaagg ccuucuccga cuucaagaag gagcaggaga gcaacaccgc cgagcuguac     2220

gcgaagaaga ucagcgagcu ggacuacaag aacaagcgga aguucuucga gcccuucucc     2280

ggcuuccgcc agaagcuccu ggacaagauc gacgagaucu ucgucagcaa gccggagcgg     2340

aagaagcccu ccggggcccu gcacgaggag accuuccgca aggaggagga guucuaccag     2400

agcuacggcg ggaaggaggg cgugcucaag gcccuggagc uggggaagau ccggaaggug     2460

aacggcaaga uggucaagaa cggcgacaug uuccgcgugg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu gcccaucuac acgauggacu ucgcccucaa gguccugccc     2580

aacaaggcgg uggugcaggg gaaggacaag aaguccggcc ugaucaagga cuggauccuc     2640

auggacgaga acuacgaguu cugcuucagc cuguacaagg acucccugau ccucauccag     2700

accaaggaca ugcaggagcc ggagcugguc uacuacgagc ggcuggacag cuccaacgcc     2760

agccucaccg uguccaagca cgacaacaag uucgagaccc ugagcaagaa ccagaagauc     2820

cuguucaaga acgccaacga gaaggaggug aucgccaagu ccaucgggau ccagaaccuc     2880

aaggucuucg agaaguacau cgugagcgcg cugggcgagg ugacgaaggc cgaguuccgc     2940

cagcgggagg acuucaagaa guga                                            2964


<210>  1409
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1409
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcgaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggagguccu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gggcgcccaa gaacagcccc      780

cuggccuuca uguucgucgc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga ggugcugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg accacucccu gagccaggac gaccugaacg agaucgcgaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaccagaac     1140

cagaucgacu cccugagcaa gcuggaguuc aaggaccacc ucaacaucuc cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggcgau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu cacgaacccc guggugcugc gggccaucaa ggaguaccgc     1380

aagguccuga acgcccuccu gaagaaguac gggaaggugc acaagaucaa caucgagcug     1440

gcgcgcgagg ugggcaagaa cuacagccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

uccaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacagc     1620

ggggagaaga ucaagaucuc cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuaca gccgguccuu cgacgacagc uacaugaaca agguccuggu guucaccaag     1740

cagaaccagg agaagcucaa ccagaccccg uucgaggcgu ucggcaacga cuccgccaag     1800

uggcagaaga ucgaggugcu ggccaagaac cugcccacca agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu ccucaacuac accaaggacu accuggacuu ccugccccuc     1980

agcgacgacg agaacaccaa gcugaacgac acccagaagg gguccaaggu gcacguggag     2040

gccaagagcg gcaugcugac guccgcgcuc cgccacaccu ggggguucag cgccaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgucauca ucgccuacgc gaacaacucc     2160

aucgugaagg ccuucagcga cuucaagaag gagcaggaga gcaacuccgc cgagcuguac     2220

gccaagaaga ucagcgagcu ggacuacaag aacaagcgca aguucuucga gcccuucucc     2280

ggcuuccggc agaaggugcu ggacaagauc gacgagaucu ucgucagcaa gccggagcgc     2340

aagaagcccu ccggcgcgcu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

agcuacgggg gcaaggaggg ggugcucaag gcccuggagc ugggcaagau ccgcaagguc     2460

aacgggaaga ucgugaagaa cggcgacaug uuccgggugg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu ccccaucuac acgauggacu ucgcccucaa ggugcugccc     2580

aacaaggccg uggcgcgcuc caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucag ccucuacaag gacucccuga uccugaucca gaccaaggac     2700

augcaggagc cggagcucgu cuacuucaac gccuucacca gcuccaccgu gagccugauc     2760

guguccaagc acgacaacaa guucgagacg cugagcaaga accagaagau ccucuucaag     2820

aacgccaacg agaaggaggu caucgccaag uccaucggga uccagaaccu gaagguguuc     2880

gagaaguaca ucgugagcgc ccugggcgag gucaccaagg cggaguuccg gcagcgcgag     2940

gacuucaaga acgagcuccg guga                                            2964


<210>  1410
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1410
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacaa cggcgacgag      420

gagaaguccg agauccugaa ggccaucaag cagaacgagg agaagcuggu gaacuaccag      480

agcgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucaucaacg uccggaacaa gaaggagucc uacgagcgcu gcaucgcgca gagcuuccug      600

aaggacgagc ugaagcucau cuuccagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggaggugcu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgucg gcaacugcuc cuucuucacg gacgagaagc gggcccccaa gaacagcccc      780

cugguguuca uguucguggc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac caaggacgac cugaacgcgc ugcucaacga gguccugaag      900

aacggcacgc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac cuacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgacg agcacucccu gagccaggac gaccugaacg agaucgccaa ggacaucacg     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaggagaac     1140

cagaucgacu cccugagcaa gcuggaguuc aaggaccacc ucaacaucuc cuucaaggcg     1200

cugaagcuga ucaccccgcu gaugcucgag gggaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggccau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu gaccaacccc gucgugcugc gggccaucaa ggaguaccgc     1380

aagauccuga acgcgcuccu gaagaaguac ggcaaggugc acaagaucaa caucgagcug     1440

gcccgcgagg ucgggaagaa ccacagccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgcggagcuc gagugcgaga agcugggccu gaagaucaac     1560

uccaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cguguacagc     1620

ggcgagaaga ucaagcucuc cgaccugcag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuaca gccgguccuu cgacgacagc uacaugaaca aggugcucgu cuucgccaag     1740

cagaaccagg agaagcugaa caagacgccg uucgaggccu ucgggaacga cuccaccaag     1800

uggcagaaga ucgaggugcu gaccaagaac cuccccacca agaagcagaa gcgcauccug     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcucgu gcugaacuac accaaggacu accuggacuu ccucccccug     1980

agcgacgacg agaacaccaa gcugaacgac acccagaagg gcuccaaggu ccacguggag     2040

gccaagagcg ggaugcucac guccgcgcug cgccacaccu ggggcuucag caccaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgugauca ucgccuacgc caacaacucc     2160

aucgucaagg cguucagcga cuucaagaag gagcaggaga gcaacuccgc cgagcuguac     2220

gccaagaaga ucagcgagcu ggacuacaag aacaagcgca aguucuucga gcccuucucc     2280

ggguuccggc agaaggugcu ggacaagauc gacgagaucu ucgucagcaa gccggagcgc     2340

aagaagcccu ccggcgcccu ccacgaggag accuuccgga aggaggagga guucuaccag     2400

agcuacggcg ggaaggaggg cgugcugaag gcccuggagc ucgggaagau ccgcaaggug     2460

aacggcaaga ucgucaagaa cggggacaug uuccgggugg acaucuucaa gcacaagaag     2520

acgaacaagu ucuacgcggu gcccaucuac accauggacu ucgcccugaa gguccugccc     2580

aacaaggccg uggcccgcuc caagaagggc gagaucaagg acuggauccu cauggacgag     2640

aacuacgagu ucugcuucag ccuguacaag gacucccuga uccucaucca gaccaaggac     2700

augcaggagc cggagcuggu guacuucaac gcguucacca gcuccacggu cagccugauc     2760

guguccaagc acgacaacaa guucgagacc cucagcaaga accagaagau ccuguucaag     2820

aacgccaacg agaaggaggu gaucgccaag uccaucggca uccagaaccu gaaggucuuc     2880

gagaaguaca ucgugagcgc ccucggggag gugaccaagg ccgaguuccg gcagcgcgag     2940

gacuucaaga acgagcugcg guga                                            2964


<210>  1411
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1411
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcgaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggagguccu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gggcgcccaa gaacagcccc      780

cuggccuuca uguucgucgc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacaccc ugcucaacga ggugcugaag      900

aacggcaccc ugaccuacaa gcagacgaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac cuacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg agcacaaccu gucccaggac gaccugaacg agaucgccaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcgcuggcca aguacgaccu caaccagaac     1140

cagaucgaca gccuguccaa gcuggaguuc aaggaccacc ucaacaucag cuucaaggcc     1200

cugaagcugg ugaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga aggucgccau caacgaggac aagaaggacu uccuccccgc guucaacgag     1320

acguacuaca aggacgaggu gaccaacccc gugguccugc gggccaucaa ggaguaccgc     1380

aaggugcuga acgcccuccu gaagaaguac gggaaggugc acaagaucaa caucgagcug     1440

gcccgcgagg ucggcaagaa ccacucccag cgggcgaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

agcaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacucc     1620

ggggagaaga ucaagaucag cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuacu cccggagcuu cgacgacucc uacaugaaca aggugcuggu guucaccaag     1740

cagaaccagg agaagcucaa ccagaccccg uucgaggccu ucggcaacga cagcgcgaag     1800

uggcagaaga ucgagguccu ggccaagaac cugcccacga agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag aacuucaagg accggaaccu gaacgacacc     1920

cgcuacaucg cccggcuggu gcucaacuac accaaggacu accuggacuu ccugccccuc     1980

uccgacgacg agaacaccaa gcugaacgac acgcagaagg ggagcaaggu gcacgucgag     2040

gccaaguccg gcaugcugac cagcgcccuc cgccacaccu ggggguucuc cgcgaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgugauca ucgccuacgc caacaacagc     2160

aucgugaagg cguucuccga cuucaagaag gagcaggaga gcaacagcgc cgagcuguac     2220

gccaagaaga ucuccgagcu ggacuacaag aacaagcgca aguucuucga gcccuucagc     2280

ggcuuccggc agaagguccu ggacaagauc gacgagaucu ucguguccaa gccggagcgc     2340

aagaagccca gcggcgcccu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

uccuacgggg gcaaggaggg gguccucaag gcgcuggagc ugggcaagau ccgcaaggug     2460

aacgggaaga ucgugaagaa cggcgacaug uuccgggucg acaucuucaa gcacaagaag     2520

acgaacaagu ucuacgccgu gcccaucuac accauggacu ucgcccucaa ggugcugccc     2580

aacaaggccg ucgcccgcag caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucuc ccucuacaag gacagccuga uccugaucca gaccaaggac     2700

augcaggagc cggagcucgu guacuucaac gcguucaccu ccagcacggu gucccugauc     2760

gucagcaagc acgacaacaa guucgagacc cuguccaaga accagaagau ccucuucaag     2820

aacgccaacg agaaggaggu gaucgccaag agcaucggga uccagaaccu gaagguguuc     2880

gagaaguaca ucgucuccgc ccugggcgag gugaccaagg ccgaguuccg gcagcgcgag     2940

gacuucaaga acgagcuccg guga                                            2964


<210>  1412
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1412
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcgaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggagguccu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gggcgcccaa gaacagcccc      780

cuggccuuca uguucgucgc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga ggugcugaag      900

aacagcaccc ugaccuacaa gcagaccaag aagcuccugg gccuguccga cgacuacgag      960

uucaaggggg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucggcg accacagccu gucccaggac gaccugaacg agaucgcgaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaccagaac     1140

cagaucgaca gccuguccaa gcuggaguuc aaggaccacc ucaacaucag cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag gggaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggcgau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu cacgaacccc guggugcugc gggccaucaa ggaguaccgc     1380

aagguccuga acgcccuccu gaagaaguac ggcaaggugc acaagaucaa caucgagcug     1440

gcgcgcgagg uggggaagaa cuacucccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

agcaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacucc     1620

ggcgagaaga ucaagaucag cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuacu cccggagcuu cgacgacucc uacaugaaca agguccuggu guucaccaag     1740

cagaaccagg agaagcucaa caagaccccg uucgaggcgu ucgggaacga cagcgccaag     1800

uggcagaaga ucgaggugcu ggccaagaac cugcccacca agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu ccucaacuac accaaggacu accuggacuu ccugccccuc     1980

uccgacgacg agaacaccaa gcugaacgac acccagaagg gcagcaaggu gcacguggag     2040

gccaaguccg ggaugcugac gagcgcgcuc cgccacaccu ggggcuucuc cgccaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgucauca ucgccuacgc gaacaacagc     2160

aucgugaagg ccuucagcga cuucaagaag gagcaggagu ccaacagcgc cgagcuguac     2220

gccaagaaga ucuccgagcu ggacuacaag aacaagcgca aguucuucga gcccuucagc     2280

ggguuccggc agaaggugcu ggacaagauc gacgagaucu ucgucuccaa gccggagcgc     2340

aagaagccca gcggcgcgcu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

uccuacggcg ggaaggaggg cgugcucaag gcccuggagc uggggaagau ccgcaagguc     2460

aacggcaaga ucgugaagaa cggggacaug uuccgggugg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu ccccaucuac acgauggacu ucgcccucaa ggugcugccc     2580

aacaaggccg uggcgcgcag caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucuc ccucuacaag gacagccuga uccugaucca gaccaaggac     2700

augcaggagc cggagcucgu cuacuucaac gccuucaccu ccagcaccgu gucccugauc     2760

gugagcaagc acgacaacaa guucgagacg cuguccaaga accagaagau ccucuucaag     2820

aacgccaacg agaaggaggu caucgccaag agcaucggca uccagaaccu gaagguguuc     2880

gagaaguaca ucguguccgc ccugggggag gucaccaagg cggaguuccg gcagcgcgag     2940

gacuucaaga acgagcuccg guga                                            2964


<210>  1413
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1413
auggcccgca uccuggccuu cgacaucggc aucagcucca ucgggugggc guucagcgag       60

aacgacgagc ucaaggacug cggcgugcgg aucuucacca aggccgagaa ccccaagacc      120

ggggaguccc uggcccugcc gcgccggcuc gcccgcagcg cccggaagcg ccuggcgcgg      180

cgcaaggccc ggcugaacca ccucaagcac cugaucgcca acgaguucaa gcugaacuac      240

gaggacuacc aguccuucga cgagagccuc gccaaggcgu acaagggcuc ccugaucagc      300

cccuacgagc ugcgcuuccg ggcccucaac gagcugcugu ccaagcagga cuucgcccgc      360

gucauccucc acaucgccaa gcggcgcggg uacgacgaca ucaagaacag cgacgacaag      420

gagaagggcg ccauccugaa ggcgaucaag cagaacgagg agaagcuggc caacuaccag      480

uccgugggcg aguaccucua caaggaguac uuccagaagu ucaaggagaa cagcaaggag      540

uucacgaacg ugcggaacaa gaaggagucc uacgagcgcu gcaucgccca gagcuuccug      600

aaggacgagc ugaagcucau cuucaagaag cagcgggagu ucggguucuc cuucagcaag      660

aaguucgagg aggagguccu guccguggcc uucuacaagc gcgcccugaa ggacuucagc      720

caccucgugg gcaacugcuc cuucuucacc gacgagaagc gggcgcccaa gaacagcccc      780

cuggccuuca uguucgucgc ccugacccgc aucaucaacc uccugaacaa ccugaagaac      840

accgagggga uccucuacac gaaggacgac cugaacgccc ugcucaacga ggugcugaag      900

aacggcaccc ugaccuacaa gcagaccaag aagcuccugg ggcugagcga cgacuacgag      960

uucaagggcg agaagggcac guacuucauc gaguucaaga aguacaagga guucaucaag     1020

gcccucgggg accacucccu gagccaggac gaccugaacg agaucgcgaa ggacaucacc     1080

cucaucaagg acgagaucaa gcugaagaag gcccuggcca aguacgaccu caaccagaac     1140

cagaucgacu cccugagcaa gcuggaguuc aaggaccacc ucaacaucuc cuucaaggcc     1200

cugaagcuga ucaccccgcu gaugcucgag ggcaagaagu acgacgaggc cugcaacgag     1260

cugaaccuga agguggcgau caacgaggac aagaaggacu uccuccccgc cuucaacgag     1320

accuacuaca aggacgaggu cacgaacccc guggugcugc gggccaucaa ggaguaccgc     1380

aagguccuga acgcccuccu gaagaaguac gggaaggugc acaagaucaa caucgagcug     1440

gcgcgcgagg ugggcaagaa cuacagccag cgggccaaga ucgagaagga gcagaacgag     1500

aacuacaagg ccaagaagga cgccgagcuc gagugcgaga agcugggccu gaagaucaac     1560

uccaagaaca uccucaagcu gcgccuguuc aaggagcaga aggaguucug cgccuacagc     1620

ggggagaaga ucaagaucuc cgaccuccag gacgagaaga ugcuggagau cgaccacauc     1680

uaccccuaca gccgguccuu cgacgacagc uacaugaaca agguccuggu guucaccaag     1740

cagaaccagg agaagcucaa ccagaccccg uucgaggcgu ucggcaacga cuccgccaag     1800

uggcagaaga ucgaggugcu ggccaagaac cugcccacca agaagcagaa gcgcauccuc     1860

gacaagaacu acaaggacaa ggagcagaag gacuucaagg accggaaccu gaacgacacg     1920

cgcuacaucg cccggcuggu ccucaacuac accaaggacu accuggacuu ccugccccuc     1980

agcgacgacg agaacaccaa gcugaacgac acccagaagg gguccaaggu gcacguggag     2040

gccaagagcg gcaugcugac guccgcgcuc cgccacaccu ggggguucag cgccaaggac     2100

cggaacaacc accuccacca cgccaucgac gccgucauca ucgccuacgc gaacaacucc     2160

aucgugaagg ccuucagcga cuucaagaag gagcaggaga gcaacuccgc cgagcuguac     2220

gccaagaaga ucagcgagcu ggacuacaag aacaagcgca aguucuucga gcccuucucc     2280

ggcuuccggc agaaggugcu ggacaagauc gacgagaucu ucgucagcaa gccggagcgc     2340

aagaagcccu ccggcgcgcu gcacgaggag accuuccgga aggaggagga guucuaccag     2400

agcuacgggg gcaaggaggg ggugcucaag gcccuggagc ugggcaagau ccgcaagguc     2460

aacgggaaga ucgugaagaa cggcgacaug uuccgggugg acaucuucaa gcacaagaag     2520

accaacaagu ucuacgccgu ccccaucuac acgauggacu ucgcccucaa ggugcugccc     2580

aacaaggccg uggcgcgcuc caagaagggc gagaucaagg acuggauccu gauggacgag     2640

aacuacgagu ucugcuucag ccucuacaag gacucccuga uccugaucca gaccaaggac     2700

augcaggagc cggaguucgu cuacuacaac gccuucacca gcuccaccgu gagccucauc     2760

guguccaagc acgacaacaa guucgagacg cugagcaaga accagaagau ccuguucaag     2820

aacgccaacg agaaggaggu caucgccaag uccaucggga uccagaaccu caagguguuc     2880

gagaaguaca ucgugagcgc ccugggcgag gucaccaagg cggaguuccg gcagcgcgag     2940

gacuucaaga acgagcugcg guga                                            2964


<210>  1414
<211>  2979
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1414
augcgcaucu ucagcgacgg ccggaacccc aaggacgggu ccagccuggc cgugacccgc       60

cgggccgcgc gcgccaugcg gcgccggcgc gaccggcucc ugaagcgcaa gacccggaug      120

caggccaagc uggucgagca cggcuucuuc ccggccgacg ccgggaagcg caaggcgcuc      180

gagcagcuga accccuacgc ccugcgggcc aagggccucc aggaggcccu gcugcccggg      240

gaguucgcgc gcgcccucuu ccacaucaac cagcggcgcg gcuucaaguc caaccggaag      300

acggacaaga aggacaacga cagcggcgug cugaagaagg ccaucgggca gcugcgccag      360

cagauggccg agcagggcuc ccggaccgug ggggaguacc ucuggacccg ccugcagcag      420

ggccaggggg uccgggcccg cuaccgcgag aagcccuaca ccacggagga gggcaagaag      480

cggaucgaca agagcuacga ccuguacauc gaccgcgcga ugaucgagca ggaguucgac      540

gcccucuggg ccgcccaggc cgcguucaac ccgacccugu uccacgaggc cgcccgggcc      600

gaccugaagg acacccuccu gcaccagcgc ccccugcggc ccgugaagcc cggccgcugc      660

acccuccugc cggaggagga gcgggcgccc cuggcccucc ccuccacgca gcgcuuccgg      720

auccaccagg aggugaacca ccuccgccug cucgacgaga accugcggga ggucgcccug      780

acccucgccc agcgcgacgc cguggugacc gcccuggaga ccaaggcgaa gcugagcuuc      840

gagcagaucc ggaagcuccu gaagcugucc gggagcgucc aguucaaccu cgaggacgcc      900

aagcgcacgg agcugaaggg caacgccacc uccgccgcgc uggcccggaa ggagcuguuc      960

ggggccgccu ggagcggcuu cgacgaggcc cugcaggacg agaucgugug gcagcuggug     1020

accgaggagg gcgagggggc gcucaucgcc uggcugcaga cccacacggg cgucgacgag     1080

gcccgcgccc aggccaucgu ggacgugucc cugcccgagg gguacggcaa ccucagccgg     1140

aaggcgcugg cccgcaucgu cccggcccug cgggccgccg ugaucaccua cgacaaggcg     1200

gugcaggccg ccggguucga ccaccacucc cagcucggcu ucgaguacga cgccagcgag     1260

gucgaggacc uggugcaccc cgagaccggc gagauccgcu ccguguucaa gcagcugccc     1320

uacuacggga aggcgcugca gcgccacguc gccuucggca gcgggaagcc cgaggacccg     1380

gacgagaagc gguacggcaa gaucgccaac cccaccgugc acaucgggcu caaccaggug     1440

cgcauggucg ugaacgcccu gauccggcgc uacggccggc ccacggaggu cgugaucgag     1500

cuggcccgcg accucaagca gucccgggag cagaaggugg aggcgcagcg ccggcaggcc     1560

gacaaccagc gccggaacgc ccgcauccgg cgcagcaucg ccgagguccu gggcaucggg     1620

gaggagcggg ugcgcggcag cgacauccag aaguggaucu gcugggagga gcuguccuuc     1680

gacgccgcgg accggcgcug ccccuacagc ggggugcaga ucuccgccgc caugcuccug     1740

agcgacgagg ucgaggugga gcacauccug ccguucucca agacccucga cgacagccug     1800

aacaaccgga ccguggccau gcgccaggcc aaccggauca agcgcaaccg cacccccugg     1860

gacgcgcggg ccgaguucga ggcccagggc ugguccuacg aggacauccu gcagcgcgcc     1920

gagcggaugc cccuccgcaa gcgguaccgc uucgcgcccg acggguacga gcgguggcug     1980

ggcgacgaca aggacuuccu ggcccgcgcc cucaacgaca cgcgguaccu gagccgcguc     2040

gccgccgagu accugcggcu cgugugcccg ggcacccgcg ugauccccgg gcagcugacc     2100

gcgcugcucc ggggcaaguu cgggcugaac gacguccugg gccucgacgg cgagaagaac     2160

cgcaacgacc accggcacca cgccguggac gccugcguga ucggggucac cgaccagggc     2220

cugaugcagc gcuucgccac ggccuccgcg caggcccgcg gggacggccu gacccggcuc     2280

guggacggga ugcccaugcc cuggccgacc uaccgggacc acguggagcg cgccguccgc     2340

cacaucuggg ugagccaccg gcccgaccac ggcuucgagg gcgccaugau ggaggagacc     2400

uccuacggga uccgcaagga cggcagcauc aagcagcggc gcaaggccga cggguccgcg     2460

ggccgggaga ucagcaaccu gauccgcauc cacgaggcca cgcagccccu gcggcacggg     2520

guguccgccg acggccagcc gcucgccuac aagggcuacg ucgggggcag caacuacugc     2580

aucgagauca ccgugaacga caaggggaag ugggagggcg aggugaucuc caccuuccgc     2640

gccuacgggg ucgugcgggc gggcggcaug gggcgccugc ggaaccccca cgagggccag     2700

aacgggcgca agcugaucau gcggcucgug aucggcgaca gcguccgccu ggagguggac     2760

ggggccgagc ggaccaugcg caucgugaag aucuccggca gcaacggcca gaucuucaug     2820

gcccccaucc acgaggccaa cgucgacgcg cggaacacgg acaagcagga cgccuucacc     2880

uacaccagca aguacgccgg gucccugcag aaggccaaga cccgccgggu gaccaucagc     2940

cccaucggcg aggugcgcga cccgggguuc aagggcuga                            2979


<210>  1415
<211>  2985
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1415
augacccugg gccucgaccu ggggaccaac agcaucggcu gggcccugau cgacuucgac       60

gacaaccagc agccccgcaa ccucaucgcc ugcggguccc ggaucuucca ggaggcggug      120

gaggccaaga cgggcacccc gaagaaccac gcccgccgga ccgcccgcgc cgcgcggaag      180

cuggucgccc gccggaagca gcgccggacc aagcugcuca accugcugau ccgccacggg      240

cuccugcccc aggacguggc cgagcggaac gcccugcucc uggacaacca ggcgaacaac      300

cccuaccagc ugcgcaagcg gggccucgac gagaagcugg agcccuucgc cuucggccgc      360

gcccuguacc accucgccca ccggcgcggg uuccagagca accggaaggc cgcguccgac      420

gaggacggca aggugaagac ggccaucagc acccugcgcg ccgagaugca ggggcggacc      480

cugggcgccu uccucgccga ccagccgacc cagcgccggc gcuacacgga ccgcgcgaug      540

uacgucgagg aguucgagca gcuguggucc aagcaggcca gcuucaccca ggagcagcug      600

acccaggccc ucaagguggc cauccacaac uccaucuucu uccagcggcc ccucaagagc      660

cagaagcacc ugguggggaa gugcaccuuc gagcccacgc gcaagcgggc cgcgaaggcc      720

auccuccuga cccagcgcuu ccggauccug caggacguca accaccucgc cgugaagaac      780

cccaucaccc gcgaguuccg gccgcugacc caggacgagc gcaacaagcu ggccgaggcc      840

cucgacaagc agaaguccau gacguggggc gcggugcgga aggucaucgg cuggaacaag      900

aaggacagcc ugcacgaggg ggagaccuuc aaccuggagg agggcaagaa ggacaagcuc      960

aucgggaacc gcaccgccug cgaccugcgg ggcgcccugg gcgaccgcug ggacgcccuc     1020

accgcgcagc agcaggacga ccuggugacg gaccugcuca ccaucgacaa caccgacggg     1080

uuccugaagc ggaugaagac ccacugggag uucgacgacg ccacggccga ggugaucgcc     1140

aagaucgagc uggagcccgg cuacgcccgc cucuccgcga aggccaugcg ggccauccug     1200

cccuaccugg agcaggggau gaucuacagc gacgccugca aggccgcggg cuacgaccac     1260

uccaacccca accagcgcac caucaccgac aagcucgggc ugccgagcaa cguccggaac     1320

cccguggugc agaaggcccu guucgagacc cgcaaggucg ugaacgccau cgugcggaag     1380

uacggcaagc ccgccgucau ccgcguggag auggcccgcg acaugaagcu guccaagcgg     1440

cagaaggagg agcuccagaa ggagcagaac aagcagaaga aggcgaacga gcuggccgag     1500

ggcauccugc gcaaggaguu cgggauccag cagcccgccc gggccgacau ccagaaguac     1560

aaccucuggc uggagugcaa ggaggugugc ccguacacgg gcaccaccau cagccgcgag     1620

augcuguucu cccccgaggu cgacguggag cacauccucc ccuacagccg gucccuggac     1680

gacagcuaca ugaacaagac ccugugcaug gcgacguuca accgccaggu gaagcucaac     1740

aacacccccu acgagaucgg gcagggcgac ccgcuggccu acgccgagau cuuccagcgg     1800

aucaagaccc ugcccuuccc caagcgccgg cgcuucgagc agaaggagau cgagaccgac     1860

aaguucaucg agcggcagcu caacgacacg cgcuacaucu gcgucgaggu gcgggacuuc     1920

cugcagcagc ugggggcccc cgucgaggug agcaagggcg aggccaccgc gaagcuccgc     1980

ggccggugga agcugaacac ccugcucucc ccggacggga gcaacgagaa guaccgcggc     2040

gaccaccggc accacgccau cgacgccgug gucaucgccc ugaccaaccg cucccuguuc     2100

cacaagcuca gccggcuguc cgcccagagc aucucccugg acaagagccg cuucgacgug     2160

cccccgcccu gggacggguu cuacgacgac guguacgaga agaucgacaa ggucaucgug     2220

ucccacgcgc ccgcccggaa gaucggcggg gcccuccacg aggacaccgc cuacggccac     2280

uucaaggagg agguggccga ccccaagacg ggcaagguca gcaucgagga ccgcuucgug     2340

uaccgcaagc cccuggcgac ccugaccgcc aacgaggugg ggaagauccg ggacgccaag     2400

guccgcgagu ucguggaggc ccggcucgcg caguucggca acaacuccaa gaccgccuuc     2460

ggggacccga acaacccccu gcugcacgcc gacggcaaga cgcccauccg caccgugcgg     2520

cucgucacca acuucaacaa ggccaccacg cacgccaucc agaagggcca ggggcgcgac     2580

uacaaguucu ucaaguacgg caacaaccac cacguggaga ucaucgagca cguggagacc     2640

gggaagcgga agggcaucuu cgucaccgcg auggaggccg cccgccgggu gcgcggggug     2700

gccaacaccg agaaguccga caucguccag cggaaccacg gcgaggagug gcgcuucgug     2760

augagccuga gcaucaacga ccuggugucc auccaggagg ccgaccaggu ccggaucaug     2820

cgcgugcagc uguucgacgc gagcaacggc aacaucaucu uccgggacca gaaguccgcc     2880

augcuggacg acaagcugag ccgcuaccag acgaaggccc accuccuggu gugccagaag     2940

aucaccaccg acccccuggg gaaccucgcc ccgugcaacg acuga                     2985


<210>  1416
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1416
augagcaagg ugcugggccu cgacaucggg gccaacucca ucggcugggc ccugaucgac       60

gacgaggaga acgacaucau cggggcgggc guccgcgugu ucaaggaggg ggugaaccug      120

aagggcguca aggaggagag caagaacgug gagcggcgcg ccgcccggcu cgcccgccgg      180

cagcacaagc gccgguccca gcgccggaac cgccuggugg cccugcucaa gacccuggag      240

auguuccccg agaacaacga ggacaucagc gacuacuacg cgcaggaccc guacgagcug      300

cggaagaagg gccucuacga gaagcugacc auguacgagu ucgggcgcgu ccugcuccac      360

aucugccggc gccggggcuu caaguccagc cgcaagacgg acaucuccga ggaggggaag      420

aucuucgagg gcaaggacgg gaucgugggc aucaaccaga cccgggacag cauccgcgag      480

ggcggguuca agaccaucgg cgaguaccug gccucccugg acccccacgu gauccggcgc      540

cggaaccgcu ucacccuccg cgacauguac gaggaggagu ucaaccugau gugggagaag      600

cagcagagcu ucuacccccu gcagcucucc aacgaguuca aggaggccau cgccgagguc      660

auccugagcc agcggaagcu gaagucccag aaggcgcucg uggggaacug cggccuggag      720

cccaacaaga agacggcccc caagagcucc ccggccuucc aguacuuccg cauccuggag      780

cagcucagcc ggauccugau cuccaucccc cacaccggcc gcuacaacga cccccugaac      840

gaggaggagc ggcgcaugcu caucgacaag cugagcguga ccgagaagcu gaaguucucc      900

gacaugcgga agaugcucaa ccugccgggg gagagcgaca ucaacaucga ggaggagggc      960

gggcgccugc ucggcaacac cacguacgcc cggcugauca aggucuucgg gaagaagcgc     1020

ugggccggcu ucagcgagga ggagaaggag cagaucuggc acaccuucca cuucgcggac     1080

aaccacgagu ggcugaagga guacgcccuc aagaaguggu ccaucacccc cgacgagauc     1140

aaggagcugg gcaagguggc ccuggagaag gaguacgccc gguucagcaa gaagucccuc     1200

aagaagauca ucccccagcu ggaggagggg cugacccucg acaaggccaa gaucgaggcg     1260

ggcuacacgc ccgaguacga gaucgagaag ucccugagcg ccuaccugcc gcuccccgag     1320

aacauccgca accccaucgu gcagcaggcc cuguacgagc ugcggcacgu cgugaacgcc     1380

gugauccgca ccuacgggaa gcccgacauc gcccgggucg agcucgcgcg cgagaugaag     1440

aagccgaagc agaagcggga gaagaugcgc agcgagcugc ggaagcgcca gaugaaguuc     1500

gaggagauca aguccauccu gaaggagcgc cugggcuuca ucgaccccaa gcgggacgag     1560

auccacaagu acaugcucug ggaggagugc cgcgagaccu gccccuacac cgggcggaag     1620

aucagccugu ccgcccugua cggcggcgac uucgaggugg agcacaucau ccccuacaag     1680

cgcagccucg acgacucccu guucaacaag acggugugcc ugcgggagga gaaccagauc     1740

aagggggacc gcaccccgua cgaggccuac agcggcgacc ggcagaagua cgaggagauc     1800

auccuccgcg uccagaagac cuccaugccc cgggggaaga agaaccuguu cgccaucaag     1860

gagcugaagg acgacuucgc ccaccgccag cucagcgaca ccgcguacau cucccgggag     1920

acgcgcaagu accugggcag caccaucaag aaggugcagg ccgugaaggg cggguccacc     1980

gccaagcugc gguacuacug gggccucaac agcauccugg ggguccgcga caucaagaug     2040

cgggacgacc accgccacca cgccguggac gcgcugguca ucgccaacac cacguacgcc     2100

uucgugaaca cccucucccg guaccacagc auggacgugg agccccacga gagccgcuuc     2160

ccccugccgu gggagcaguu ccggaacgac gccgaguccg ucgugcgcaa caucuucgug     2220

agccaccggc ugggcaagaa ggccaccggg cagcuccacg aggagaccua cuacggcaag     2280

auccgcaaca cggacggcga gcccuacuuc gucgugcgca agaagcuggg gacccugacc     2340

gcgaagcaga ucgccaacau cguggacccc gucgugaaga cccagguguu cgagcggcuc     2400

gaggagaagg gcaucgacau cgaguccagc aacaagaucc ccgccgacgc cuucgucgag     2460

acgcuguaca ugccggaccg caagaccccc aucaaguccg ugcgggugaa gguccccagc     2520

accaccauga uccagcugua caaggacaag gagcucuacg uggacccggg guccaaccac     2580

cacaucguga ucuucgagaa cgaggagggc aagcgcgacg ggaucgucgu gagccuguuc     2640

gaggccgcgc agcggcugcg ccggaacgag cccgugaucc agaagacgug gaagcccgag     2700

uggcacuucg ucaugucccu cagcaucgac gagcugaucc ugcucgacgu ggacgagaag     2760

aacaucgacu ggggcgacac cggccuggcc cugcgcuacu ccaaggcccu cuaccgggug     2820

cagaagaugg acgucaacaa gaucaucacc cugcgccacc accugguggc ccggcucaag     2880

gacgacgagg ggaaggaguu cggccgcgag caccggaucc ccagcacccu gcgcgggcug     2940

aaggugaaga ucgaccacau cggccggcuc cgccgggccu acgacuga                  2988


<210>  1417
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1417
augaagguga ucggcuucga caucgggauc aacagcaucg gcugggccuu cgucgagaac       60

gacgagcuga aggacugcgg ggugcgcauc uucaccaagg ccgagaaccc caagaacaag      120

gagucccucg cgcugccgcg gcgcaacgcc cggagcuccc gcaagcggcu gaagcgccgg      180

aagcuccgcc ugaucgccau gaagcacauc cuggccaagg agcucaaccu gaacuacaag      240

gacuacaucg ccaacgacgg cgagcugccc aaggcguacg aggggaagcu caucagcccc      300

uacgagcugc gguacaaggc ccugaaccag aagcucgagg ccaaggaccu ggugcgcguc      360

auccugcaca ucgccaagca ccggggcuac augaacaaga acgacaagaa guccaacgac      420

gcggagaagg gcaagauccu cagcgcccug aagaacaacg cccugaagcu cgagaaguac      480

caguccgugg gggaguacuu cuacaaggag uucuuccaga aguacaagga gaacaccaag      540

gacuucauca agauccgcaa caucaaggac aacuacaaca acugcgugcu ggccagcgac      600

cuggagaagg agcucaagcu gauccuggag aagcagaagg aguucggcua cuccuacagc      660

aacgacuuca ucgaggaggu cauccaggug gccuucuucc agcggccccu caaggacuuc      720

ucccaccugg uggggaccug caccuucuuc gaggaggaga agcgcgcgug caagaacagc      780

uacuccgccu gggaguucgu cgcccugacc aagaucguga acgagcucaa gagccuggag      840

aagaucuccg gcgagcugau cagcacccag acgaucaacg agauccucaa cuacauccug      900

gacaaggggu ccaucaccua caagaaguuc cggagcuaca ucaaccugua cgaguccauc      960

aaguucaaga gccucaagua cgacaaggac aacgccgaga gcgccaagcu gaucgacuuc     1020

cgcaagcugg uggaguucaa gaaggcgcuc ggcgagcacu acuucaaccg gcaggagcug     1080

gaccagaucu ccaccuacau cacccugauc aaggacaacg ucaagcucaa gacggcccug     1140

gagaaguaca accugagcaa cgagcagauc aacaaccucc uggagaucga guucaacgac     1200

cacaucaacc uguccuucaa ggcccucagc augauccugc cgcugaugaa ggagggcaag     1260

aaguacgacg aggccugcga gaucgccaac cucaagccca agaccaccaa ccagaagcag     1320

gacuuccugc ccgcguucug caacuccauc uucgugcacg agcugagcaa ccccaucgug     1380

aaccgcgcca ucuccgagua ccggaagguc cugaacgccc uccugaagaa guacgggaag     1440

gugcacaaga uccaccugga gcucgcccgc gacaucggcc ugagcaagaa ggcccgggag     1500

aagaucgaga aggagcagaa ggagaaccag gcgaucaaca ccugggcccu gaacgagugc     1560

gagaacaugg ggaucaaggc caacgccaag aacauccuca agcugaagcu guggaaggag     1620

cagaaggaga ucugcaucua cuccggcaag aagaucagca ucgagcaccu caaggacgag     1680

aaggcgcugg agguggacca caucuacccg uacucccgca gcuucgacga cuccuacaug     1740

aacaaggucc ugguguucac gaaggagaac caggagaagc ucaacaagac ccccuucgag     1800

gccuucggca agaacaucga gaaguggaac aagauccaga cccuggccca gauccugccc     1860

uacaagaaga agaacaagau ccucgacgag aacuucaagg acaaccagca gcaggacuuc     1920

aucagccgca accugaacga cacccgguac aucgccacgc ugaucgccaa guacaccaag     1980

gaguaccuca acuuccugcc ccugaacgag aacgagaaca ucaaccucaa guccggggag     2040

aagggcagca agauccacau ccagaccgug aacgggaugc ugaccuccgu ccugcgccac     2100

acguggggcu ucgacaagaa ggaccggaac aaccaccucc accacgcgcu ggacgccauc     2160

aucguggccu acagcaccaa cucccugauc aaggccuuca gcgacuucaa gaagaaccag     2220

gagcuccuga aggccaagcu guacgcgaag gagcucacca acgacaacua cauccaccag     2280

gccaaguucu ucgagccguu cgagggguuc cgcgagaaga uccugaacaa gaucgacgag     2340

aucuucguga gcaagccgcc ccggaagcgc gcccggggcg cccugcacaa ggagaccuuc     2400

uacuccgaga agaagaugau caagaaguac aacagcaagg agggccucaa caucgcccug     2460

gacugcggga agauccgcaa gaucggcacg aaguacgucg agaacgacac cauggugcgg     2520

gucgacaucu ucaagaagca gaacaaguuc uacgcgaucc ccaucuacac cauggacuuc     2580

gcccugggga uccuccccaa caagaucgug gugaucggca aggacaagaa caacaacccc     2640

aagcaguggc aggagaucga cgaguccuac gaguucugcu ucagccugua caagaacgac     2700

cugauccucc ugcagaagaa ggacaugcag gaggccgagu ucgccuacua caacggguuc     2760

gacaucgacu ccagccgcau caacaucaag aagcacgaca acaaguucga gaaccugacc     2820

uccaaccaga agcuccuguu cacgaacaag gagaagaacc ggaccggcau ccagaaccug     2880

aagaucuucg agaaguacau cgucacgccc cucggcaaca agaucaaggc ggacuuccag     2940

ccccgccaga acaucagccu gaaguccagc aagaagaacg ggcuguga                  2988


<210>  1418
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1418
augaagaucc ugggcuucga caucgggauc aacagcaucg gcugggccuu cguggagaac       60

gacgagcuca aggacugcgg gguccgcauc uucaccaagg ccgagaaccc caagaacaag      120

gagucccugg cgcugccgcg gcgcaacgcc cggagcaacc gccggcgccu caagcggcgc      180

aagucccggc ugaucgccau caagcacauc cuggccaagg agcucaaccu gaacuacaag      240

gacuacaucg ccaacgacgg cgagcugccc aaggcguacg aggggaagcu caccaacgug      300

uacgagcugc gcuacaaggc ccugaaccag aagcucgagg ccaaggaccu ggcccgggug      360

auccugcaca ucgcgaagca ccgcggcuac augaacaaga acgagaagaa guccaacgac      420

agcgagaagg gcaagauccu cagcgcccug aagaacaacg cccugaagcu cgagaaguac      480

caguccgucg gggaguacuu cuacaaggag uucuuccaga aguacaagga gaacaccaag      540

aacuucauca agauccggaa caccaaggac aacuacaaca acugcgugcu ggccagcgac      600

cuggagaagg agcucaagcu gauccuggag aagcagaagg aguucggcua cuccuacaac      660

gacgacuuca ucaaggagau caucaacauc gccuucuucc agcgcccccu caaggacuuc      720

agccaccugg ugggguccug caccuucuuc gaggaggaga agcgggcgug caagaacagc      780

uacuccgccu gggaguucgu cgcccugagc aagaucauca acgagcucaa gcgccuggag      840

aagaucuccg gcgagcugau cagcgcccag accaucaacg agauccucaa ccacauccug      900

gacaagggga acaucacgua caagaaguuc cgguccugca ucaaccugca cgagagcauc      960

gaguucaagu cccucaacua cgacaaggac aacgccgaga gcgcgaagcu gguggacuuc     1020

aagaagcuga ucgaguucaa gaaggcccuc ggcgagcaca gcuucaaccg ccaggagcug     1080

gacgagaucg ccacccacau cacccugauc aaggacaacg acaagcucaa gaccgcccug     1140

gagaaguaca accugaacaa cgagcagauc aacgcccucc uggagaucga guucaacgac     1200

cacaucaacc uguccuucaa ggcgcucaac cugauccugc cccucaugcg ggagggcaag     1260

cgcuacgacg aggccugcga gaucgugaac cucaagagca agacguccaa ccagaagcag     1320

gaccugcugc cggccuucug cgacagcauc uucucccagg agcugagcaa ccccgucgug     1380

aaccgggcca ucuccgagua ccgcaaggug cugaacgccc ugcucaagaa guacgggaag     1440

guccacaaga uccaccugga gcuggcgcgc gacgugggcc ucagcaagaa ggcccgggag     1500

aagaucgaga aggagcagaa ggagaacaag gagaucaacg acugggcccu ggacgagugc     1560

gagaaguucg ggcugaaggc caacaacaag aacauccuca agcugaagcu guggaaggag     1620

cagaaggaga ucugcgcgua cuccggcaag aagaucagca ucgagcaccu caaggacgag     1680

aaggcccugg agguggacca caucuacccc uacucccgca gcuucgacga cuccuacaug     1740

aacaaggucc ugguguucac caaggagaac caggagaagc ucaacaagac ccccuucgag     1800

gccuucggca agaacaucga gcgguggaac aagauccagg cccuggccca gaaccugccg     1860

uacaagaaga agaacaagau ccucgacgag aacuucaagg acaagcagca gcaggacuuc     1920

aucagccgca accugaacga cacccgguac aucgcgacgc ugaucgccaa guacaccaag     1980

gaguaccuca acuuccugcc ccugaacgag aacgagaaca ccaaccucaa guccggggag     2040

aagggcagca agauccacgu gcagaccauc aacgggaugc ugacguccgu ccugcgccac     2100

accuggggcu ucgacaagaa ggaccggaac aaccaccucc accacgcccu ggacgccauc     2160

aucguggccu acagcaccaa cuccaucauc aaggcguuca gcgacuucaa gaagaaccag     2220

gagcugcuca aggccaagcu guacgccaag gagcugacca gcgacaacua caagcaccag     2280

gccaaguucu ucgagcccuu cgagggguuc cgcgagaaga uccucaacaa gaucgacgag     2340

aucuucgugu ccaagccccc gcggaagcgc gcccggggca cgcugcacaa ggagaccuuc     2400

uaccccgagg agaccaugau caagaaguac aacagcaagg agggccugaa caucgcgcuc     2460

aacugcggga agauccgcaa gaucggcacc aaguacgucg agaacgacac gauggugcgg     2520

cuggacaucu ucaagaagca gaacaaguuc uacgccaucc ccaucuacac cauggacuuc     2580

gcccugggga uccuccccaa caagaucgug aucgccggca aggacaagaa caacaacccg     2640

aagcaguggc aggagaucga cgaguccuac gaguucugcu ucagccugua caagaacgac     2700

cugauccucc ugcagaagaa ggacaugcag gaggcggagu ucgccuacua caacuccuuc     2760

gacaucgaca gcucccgcau caacaucaag aagcacgaca acaaguucga gaaccugacc     2820

gacaaccaga agcuccuguu caccaaccag gagaagaacc ggacggggau ccagaaccug     2880

aagaucuucg agaaguacau cgucacgccc cucggcgaca agaucaaggc cgacuuccag     2940

ccccgccaga acaucagccu gaagaccucc aagaaguacg gccuguga                  2988


<210>  1419
<211>  2991
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1419
augaugggca acuucacccu ggggcucgac cugggcacca acagcaucgg gugggcccug       60

aucgacuucg acgacaacca gcagccccag aaccucaucg ccugcggcuc ccgcaucuuc      120

caggaggcgg uggaggccaa gacggggacc ccgaagaacc acgcccggcg cgccgcccgg      180

gcggcccgca agcuggucgc ccggcgcaag cagcggcgca ccaagcugcu caaccugcug      240

gugugccacg accuccugcc ccaggacgag gccgagcgga acgcgcugcu ccuggacaac      300

caggccaaca accccuacca gcugcgcaag cggggccucg acgagaagcu ggagcccuuc      360

gccuucggcc gcgcccugua ccaccucgcc caccggcgcg gguuccagag caaccggaag      420

gcggccuccg acgaggacgg caaggugaag accgccauca gcgcccugcg cgccgagaug      480

caggggcaca cgcugggcgc guuccucgcc gaccagccga cccagcggcg ccgguacacc      540

gaccgcgcca uguacgccga ggaguucgag cagcuguggu ccaagcagac caucuucgcc      600

cccgagcagc ugacgcaggc gcucaagguc gagaucaaga acagcaucuu cuuccagcgg      660

ccccugaagu cccagaagca ccuggugggg aagugcaccu ucgagcccac ccgcaagcgc      720

gccgccaagg ccauccuccu ggcccagcac uuccggaucc ugcaggacgu gaaccaccuc      780

gcggucaaga acccgaucac ccgcgaguuc cggccccuga cgcaggacga gcgcaacaag      840

cuggccgagg cccucgacaa gcagaagggc augaacugga gcgccgugcg gaaggugauc      900

ggcuggaaca agaaggacuc ccugcacgag ggggagaccu ucaaccugga ggagggcaag      960

aaggacaagc ucaucgggaa ccgcaccgcg ugcgaccugc ggggcgcccu gggcgaccgc     1020

ugggacgccu ucaccgcgcc ccagcaggac gaccucguca cggaccugcu gaccaucgac     1080

aaccccgacg gguuccucaa gcggaugaag acccacuggg gcuucgacga cgccaccgcc     1140

gaggcccugg ccacgaccga gcuggagccg ggguacgccc gccucagcuc caaggccaug     1200

cgggcgaucc ugccccaccu ggagcagggc augaucuaca gcgacgccug caaggccgcc     1260

ggguacgacc acuccaaccc caaccagcgc accaucaccg acaagcucgg ccugcccagc     1320

aacgugcgga acccgguggu ccagaaggcc cuguucgaga cgcgcaaggu ggugaacgcg     1380

aucguccgga aguacggcaa gcccgccgug auccgcgugg agauggcccg ggacaugaag     1440

cuguccaagc gccagaagga ggagcuccag aaggagcaga acaagcagaa gaaggccaac     1500

gagcuggcgg aggggauccu gcggaaggag uucggcaucc agcagcccgc ccgcaccgac     1560

auccagaagu acaaccucug gcuggagugc aaggaggucu gccccuacac cgggaccgug     1620

aucagccgcg agaugcugua cuccccggag guggacgucg agcacauccu ccccuacagc     1680

cggucccugg acgacagcua caugaacaag acgcugugca uggccaccua caaccgccag     1740

accaagcuca acaacacccc cuacgagauc ggccaggggg accccguggc cuacgccgag     1800

aucuuccagc ggaucaagac gcugccguuc cccaagcgcc ggcgcuucga gcagaaggag     1860

aucgagaccg acaaguucau cgagcggcag cugaacgaca cccgcuacau cugcguggag     1920

guccgggacu uccuccagca gcugggcgcg cccguggagg ucagcaaggg cgaggccacc     1980

gccaagcugc gcgggcggug gaagcucaac acgcugcugu cccccggcgg gggcaacgag     2040

aagaaccgcg ccgaccaccg gcaccacgcc aucgacgcgg uggugaucgc ccucaccaac     2100

cgcagccugu uccacaagcu gucccggcuc agcgcccagu ccaucagcuu cgacaagucc     2160

cgcuucgacg ucccgccgcc cugggacgac uucuacgacg acguguacga gaagaucgac     2220

aaggugaucg ucagccacgc ccccacccgg aagaucuccg gggcccugca cgaggcgacc     2280

gccuacggcc acuucaagga ggagaucgcc gaccccaaga ccggcaaguu cagcaucgag     2340

gaccgcuucg uguaccggaa gccccugacg acccucaccg ccaacgaggu ggggaagauc     2400

cgcgacgcga agguccggga gcugguggag gcccgccugg cccaguucgg caacaacucc     2460

aaggccgccu ucggggaccc caacgagccg cuccugcacg cggacggcaa gacccccauc     2520

cgcacgaucc ggcugaccac caacuucaac aagaccacga cccacgccau ccagaagggc     2580

caggaccgcg acuacaaguu cuucaaguac gggaacaacc accacgugga gaucaucgag     2640

cacgucgaga ccggcaagcg gaaggggauc uucgugaccg ccauggaggc cgcccgccgg     2700

gcgcgcggcg uggccaacac ggagaagucc aacaucgucc agcggaacca cggggacggc     2760

uggaaguucg ugaugagccu cgccgugaac gacauggucc gcaccgagga cacccaggcc     2820

aucuaccggg ugcagaagcu ggacggcagc aacaaccgcu ucacccugcg gucccacaac     2880

gccgcgacgc ucgacgacaa gcagaccggg guggacaaga cccccaaccu guucaagggc     2940

accaagauca ucgucgaccc ccuggggaac cucacgcagu gcaacgacug a              2991


<210>  1420
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1420
augaaggugc uggucgacgc cggccucaug cccagcgacc ccgccgcggc caaggcccug       60

gagacccugg accccuacga gcuccgcgcc accgggcugg acgugaugcu gccccucacg      120

caccugggcc gggccguguu ccaccugaac cagcgccggg gguucaaguc caaccgcaag      180

accgaccggg gcgacaacga gagcgggaag aucaaggacg cgaccaagcg ccucaccugg      240

gccaugcagg aggccggcgc ccggacguac ggcgaguucc ugcacaugcg ccgggcgcgc      300

gccuccgacc cccggcaggu cccgagcgug cgcacccggc uguccguggc cagccgcggg      360

ggccccgacu ccaaggagga ggccggguac gacuucuacc ccgagcgggc ccaccucgag      420

gacgaguucc acgcgcugug gagcgcccag gccgccuucc accccgaccu gaccugcgag      480

cuccgcgacg ucguguucga gaagaucuuc uaccagcggc cgcugaagga gcccaaggug      540

ggccugugcc ucuucaccgc cgaggagcgc cugcccaagg cgcacccccu gacggggcgg      600

cgcguccucu acgagaccgu gaaccagcug cgggugaccg ccgacggccg caccacgcgc      660

ccgcugaccc ucgaggagcg ggacaugauc gucuacgccc uggacaacaa gaagcccgug      720

aagucccuga gcuccaugca ccucaagcug guggcccugg gcaagcucgu caagcugaag      780

gacggggagc gcuucacccu ggagagcggc gugcgggacg ccaucgcgug cgaccccauc      840

cgcgccacca uggggcaccc cgacggcuuc ggcccggccu ggucccggcu cgacuggcag      900

gcccaguggg cccugaucga gaagcugcgc caggugcaga gcgacgacga cuucgcgucc      960

cucgucgccu ggcugggggc cacgcacaac cugagcgagg accacgccaa gcggguggcg     1020

aaccuccacc uccccgaggg cuacgggcgc cugggccuca ccgccaccga ggccauccug     1080

ugcaaccuga aggccgaggu gugcaccuac ucccaggccg ucgagcggug ggggaagcac     1140

cacagcaacc agcgcacggg cgagugccuc gaggcgcugc ccuacuacgg cgaggugcug     1200

gaccggcacg ugauccccgg gaccuacaac gaggacgacg acgacgucac ccgcuacggc     1260

cggaucacca acccgacggu gcacaucggg cucaaccagc ugcgccggcu ggucaaccgc     1320

aucaucgcca ccuacggcaa gcccgaccag aucguggugg agcuggcccg ggagcucaag     1380

caguccgagg cccagaaguc cgaggcccug aagaagaucc gcgacaccac cgcggacgcc     1440

auccggcgcg ggcgccagcu ggaggagauc ggccagaaga acacgggcgc caaccggcuc     1500

cugcugcgcc ucugggagga ccuggggccc gccgucggcc cccggugcug cccguacacc     1560

gggaagccca ucagcgugac caugcuguuc gacggcgccu gcgacgugga ccacauccuc     1620

cccuacagcc gcacccugga cgacagcuuc gcgaaccgga cgaucugccu gcgcgaggcc     1680

aaccggcaga agggggaccg cacccccugg gaggccuggg gcggcuccga ccagugggac     1740

gccaucgagg cgaaccucaa ggaccugccg gagaacaagc ggaagcgcuu cgcccccgac     1800

gccauggagc gguucgaggg ggagcgcgac uuccuggacc gggcccucgu cgacacccag     1860

uaccuggccc gcaucagccg ggcguaccug gacacccucu ucacggaggg cgggcacgug     1920

uggguggucc ccggccgcau gaccgagaug cugcggcgcc acuggggccu gaacgcccuc     1980

cuguccgaca aggaccgcgg ggccggcaag gugaagaacc ggaccgacca ccggcaccac     2040

gccaucgacg ccgcggugau cgccgccacc gaccacagcc uggucaaccg caucuccaag     2100

gccgccgggc agggcgagag cgcggggcag uccgccgagc ucaucgcccg cgacaccccg     2160

gagcccuggg agggcuuccg gaacgacauc agcgcccaga ucgcgcgcau cguggugucc     2220

caccgggccg accacggccg caucgacguc gaggggcgga agcacggcaa ggacagcacg     2280

gccgggcgcc ugcacaacga caccgccuac ggcaucgugg acgaccacac cguggucucc     2340

cggacgcccc ugcucagccu gaagcccggg gacaucgagg ugaccaccaa gggcaagaac     2400

auccgcgaca cccagcugca gaaggcccuc uccguggcga ccacgggcaa ggacgggaag     2460

ggcuucgagg aggcccugcg gaacuucgcc gagaaggagg ggccguacca gggcauccgc     2520

cggguccgcc ugaucgagac ccuccagagc uccgcccggg uggagguggg gagcgacgag     2580

gacggccagc cccugaaggc cuacaagggc gacuccaacc acugcuacga ccuguggaag     2640

cuccccgacg ggaaggucgu gccccacgug gucagcaccu acgaggcgca cgccggcacc     2700

gacaagcgcc cgcaccccgc cgccaagcgg auccugcgcg uguucaagaa ggacauggug     2760

gccaucgagc aggacgacca gacggcgauc uucuacgucc agaagcugga ccgggccaac     2820

gggcuguucc uggcgcccca ccuggaggcc aacgccgacg cccgccaccg gaaccccaac     2880

gacgccuuca aguuccucca gaugggcgcc gggagcgugg ugcgcaacaa gcugcgccgg     2940

gucuacgugg acgagaucgg ccgcaucaag gacccgggcc cgcccaagca cuga           2994


<210>  1421
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1421
augaagaucc ugggcuucga caucgggauc aacagcaucg gcugggccuu cguggagaac       60

gacgagcuca aggacugcgg gguccgcauc uucaccgagg ccgagaaccc cuccaacaag      120

gagagccugg cgcugccgcg gcgcaacgcc cgguccagcc gccggcgccu caagcggcgc      180

aaggcccggc ugguggccau caagcgcauc cuggccaagg agcucaagcu gaacuucaag      240

gacuacgugg cggccgacgg cgagcugccc aaggccuacg aggggaagcu caccuccauc      300

uacgagcugc gguacaaggc ccugauccag cagcucgaga cgaaggaccu ggcgcgcguc      360

auccugcaca ucgccaagca ccggggcuac augaacaaga acgagaagaa guccaacgac      420

accaagaagg gcaagauccu cagcgcccug aagaacaacg cccugaagcu cgagaacuac      480

cagagcgugg gggaguacuu cuacaaggag uucuuccaga aguacaagga gaacaccaag      540

aacuucauca agauccgcaa caccaaggac aacuacaaca acugcgugcu guccagcgac      600

cuggagaagg agcucaagcu gauccuggag aagcagaagg aguucggcua caacuacucc      660

gaggacuuca ucaacgagau ccucaagguc gccuucuucc agcggccccu gaaggacuuc      720

agccaccugg ugggggcgug cacguucuuc gaggaggaga agcgcgccug caagaacucc      780

uacagcgccu gggaguucgu ggcccucacc aagaucauca acgagaucaa gucccuggag      840

aagaucagcg gcgagaucgu ccccacccag accaucaacg agauccugaa ccucauccug      900

gacaaggggu ccaucacgua caagaaguuc cggagcugca ucaaccugca cgaguccauc      960

agcuucaaga gccucaagua cgacaaggag aacgccgaga acgcgaagcu gaucgacuuc     1020

cgcaagcugg uggaguucaa gaaggcccuc ggcgugcacu cccugagccg gcaggagcug     1080

gaccagaucu ccacccacau cacccucauc aaggacaacg ucaagcugaa gaccgugcug     1140

gagaaguaca accucagcaa cgagcagauc aacaaccugc uggagaucga guucaacgac     1200

uacaucaacc ucuccuucaa ggcccugggc augauccugc cgcucaugcg cgaggggaag     1260

cgguacuacg aggccugcga gaucacgaac cugaagccca agaccgugga cgagaaggag     1320

gacuuccugc ccgccuucug cgacagcauc uucgcgcacg agcuguccaa ccccgucgug     1380

aaccgcgcca ucagcgagua ccgcaaggug cucaacgccc ugcugaagaa guacggcaag     1440

guccacaaga uccaccucga gcuggcccgg gacguggggc uguccaagaa ggcccgcgag     1500

aagaucgagg accagcagaa gaacaacaag gcgaucaacg acugggcccu caaggagugc     1560

gagaacaucg gcaucaaggc cagcgccaag aacauccuga agcugaagcu cuggaaggag     1620

cagaaggaga ucugcaucua cuccggcaac aagaucagca ucgagcaccu gaaggacgag     1680

aaggcgcugg agguggacca caucuacccg uacucccgga gcuucgacga cuccuucauc     1740

aacaaggucc ucguguucac caaggagaac caggagaagc ugaacaagac ccccuucgag     1800

gccuucggga agaacaucga gaaguggagc aagauccaga cgcuggccca gaaccucccc     1860

uacaagaaga agaacaagau ccuggacgag aacuucaagg acaagcagca gcaggacuuc     1920

aucucccgca accugaacga cacccgguac aucaccaccc ucaucgccaa guacacgaag     1980

gaguaccuga acuuccugcc ccucagcgag aacgagaaca ccaaccugaa guccggcgag     2040

aaggggagca agauccacau ccagaccauc agcggcaugc ugaccuccgu gcuccgccac     2100

acgugggggu ucgacaagaa ggaccggaac aaccaccucc accacgcccu ggacgcgauc     2160

aucgucgccu acagcaccuc cagcaucauc aaggccuucu ccgacuucaa gaagaaccag     2220

gagcuccuga aggcccgcuu cuacgccaag gagcugacca gcgacaacua caagcaccag     2280

gugaaguucu ucgagccguu caagggcuuc cgggagaaga uccucuccaa gaucgacgag     2340

aucuucguca gcaccccgcc ccgcaagcgg gcgcgcggcg cccugcacga ggagacguuc     2400

uacuccgagg acaagaugau caagaaguac aacagcaagg aggggcugca gaucgcccuc     2460

uccugcggcc gggugcgcaa gaucgggacc aaguacgugg agaacgacac caugguccgg     2520

guggacaucu ucaagaagca gaacaaguuc uacgccaucc ccaucuacgu gauggacuuc     2580

gcccugggca uccugcccaa caagaucguc aucaccggga aggacaagaa caacaacccc     2640

aagcaguggc agacgaucga cgagagcuac gaguucugcu ucucccucua caagaacgac     2700

cugauccugc uccagaagaa gaacaugcag gagcccgagu ucgcguacua caacagcuuc     2760

gacaucgacc gcucccggau caagaucaag aagcacgaca acaaguucga gaaccugacc     2820

agcaaccaga agcugcuguu caccaacaag gacaagggca agaaccgcac cggcauccag     2880

aaccugaaga ucuucgagaa guacaucgug acgccccugg gggacaagau caaggccgac     2940

uuccagccgc gggagaacau cucccucaag accagcaaga agcacggccu guga           2994


<210>  1422
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1422
augggcaacu acacccuggg gcucgacauc ggcagcaacu ccaucgggug ggcccuggug       60

gaggacgccg ucgaggcgcc ccgcaucgug gacaugggcg ugcgggucuu cccggagggg      120

guggaccgcg acaccaaggg ccuggagaag uccaagaacg ccacgcggcg cgaggcccgg      180

ggcgcccgcc ggacccacca gcgccggcgc cagcggaagc agcgccucgu gcgggcccug      240

cgccaggucg ggcugcuccc cugcgacaag gcggaggugc ggagccuguu ccagaccgac      300

cccuacgaga uccgcaagcg gggccuggac caccgccucg agcuguacga gaucgggcgg      360

gccuucuacc accugagcca gcgccggggc uucaagucca accgcaagag cggggcccuc      420

aaggaggacg gcaccgugaa gaaggaggcc ggcgagcugc agcgcaagau cgacgaggcg      480

gggugccgga cgaucggcga guacuucgcc gggcuggacc ccgagcagca gcgcauccgg      540

ggccgcuaca ccuuccgguc cauguaccag aaggaguucg accuccugug ggccaagcag      600

gucgaguucu acccgaaggu gcugaccgag gaccuccgca gcaaggugcg ggacgagauc      660

aucuucuucc agcgcccccu gaaguccgcc aaggaccuga ucggcgacug cgagcucgag      720

cccaccgaga agcggugccc ccgcgccgac ugguacgcgc ggcgcuuccg gcugcugcag      780

gacaucaaca accucaucau ccagaacccg gacgggacgg agcagaagcu gagccccgac      840

cagcgcgccg cccugcucgg cgagcugggg cagcgggaga agcugaccuu cgacaacauc      900

cgcgccaagc ucggccugcu ggagacccag aaguucaacg ccgaguaccg gaucgggcgc      960

aagggcaaga aggucgaggc gaucaagggc gacguguucg uguggaccau gcgggggaag     1020

uccgucuucg gccccaaggc cugggacgcc auggccgacg agcgcaaggu gagccucaac     1080

gagcuguucg uggagcugga ggacgacgac cucgcccgca agcuggagac ggaguacggg     1140

cugaccgaca agcaggucca ggcggccaug aaggccuccc ucccccaggg cuacaugagc     1200

uucucccgga aggccgugcu gaagcugcuc ccguucaugg aggccggggc gcugacccac     1260

gaggccaucg accaggccgg cuaccgcaag gaggagccca cccagacgga cgccaucgcg     1320

cgggugagcu ucccgcccga ccugcgcaac ccgaucgucc agaaggcccu cuucgaggug     1380

cggaaggugg ucaacgcccu ggugcgcgag uacggcaagc ccacccggau caagaucgag     1440

auggcccgcg acguccaggg guccaagcgg cagcgcgagg agcugcacug gcggaacgag     1500

gacaaccgca agaagaacga ggaggugcgg gaccgccucc ggaccgacau ccacaucgac     1560

gaccccagcc gcgacgacgu gaucaaguac aagcuguggc aggaguccgg caagaugugc     1620

cccuacaccg ggcggcagau cugccaggag gcccuguucg gcccgaaccc cgaguuccag     1680

gucgagcaca uccugcccua cgaccgcagc cucgacgaca gcuacaugaa ccugacgcug     1740

ugcgaggugc acgagaacau ccacgggaag cggaaccaga cccccuucga ggcguacggc     1800

uccggcgccg agcgcuacga gcagaucaag cagcggauca aggugcuccc guggcccaag     1860

cgccagaagu uccugcagca ggagaucaac cuggacagcc acaucucccg cgagcucaac     1920

gacacccggu acaucugccg cgagaccguc gccuaccuga agcagcuggg ggugcacgug     1980

cggggcacgc gcgggaagau caccgccgag cuccggcacc aguggggccu ggacggcauc     2040

uucagcgagc uggggguccg ccgggacgac gaccaccgcc ggcacgccgu ggacgcgcuc     2100

guggucgccg ugaccgacaa cgagcaccuc cgccggcugg ccguguccaa guacguccug     2160

gccggccccc gcuucgagac ccccuggagc gccuuccggg acgaggcgcg cgggaacgug     2220

aagcggauga acguguccca ccgcgucugc cggaagguga gcggcgcccu gcacgaggag     2280

acgaacuacg ggccgaccgg cgugcgcgac caggccggcc aggacaucua cgccuaccgg     2340

aagaagcugg aggaccucac cacccccaug gucgagaaga ucguggaccc cgugguccgc     2400

gacaucgugc gccagcggcu gcugaugaag gggaucgacc cggcggccgc cgaccgcaag     2460

auccccaagg acguguggaa ggagccccuc uacaugcggc gcacgaaguc cgacaagaug     2520

auccagaucc ggcgcguccg gauccacgac gugaagaaca gcaucaucgu gcugaccgac     2580

gaguccggcc gccccuaccg ggccgucgag cccgggggca accaccacgu ggagguguuc     2640

gaguacgucc acgggaccaa gaccggcacg cgcgaggccg agguggugac cauguucgag     2700

gcgguccggc gcagccgggc ccgcgccccc guggugcggc gcgaccacgg ccccgggacc     2760

cgguucgucu gcucccuggc caucaacgac auggugauga ugcccgacaa gagcggcaag     2820

auggaccucu uccgcaucca gaagaugucc gccagcaagc aggucuacuu ccggcaccac     2880

accgcggccc gcaucgacuc cgagcggacc cugauccgca agcaggccca ccuguucaac     2940

ggguacaagg ugagcgugga cccgacgggc gccaucugcc gcgccaacga cuga           2994


<210>  1423
<211>  3000
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1423
augaagaucc ugggcuucga caucgggauc aacagcaucg gcugggccuu cguggaggac       60

aaccagcucc aggacugcgg gguccgccug uucaccaagg ccgaggaccc caagaccaag      120

gagucccugg cgcucccgcg gcgcaacgcc cggagcaacc gccggcgccu gggccggcgc      180

cggucccgcc ugaucgcccu caagcacauc aucagcaagg ggcugaagcu gaacuaccag      240

gacuacaucg ccaacgacgg cgagcucccc aaggccuacg agggccggcu gaucuccccc      300

uacgagcugc gcuacaaggc gcucaacgag aagaucgagc ccaaggaccu ggcccgggug      360

auccugcaca ucgccaagca ccgcggguac augaacaaga acgagaagaa guccagcgac      420

aacgagaagg gcaagauccu cagcgcccug aagacgaacg cgcugaagcu cgagaaguac      480

caguccgugg gggaguacuu cuacaaggag uucuuccaga aguaccggga gaacaccaag      540

gacuucauca acauccgcaa caaggagggc agcuacgaga acugcguccu ggccuccgac      600

cuggagaagg agcucaagcu gauccuggag aagcagaagg agugggggua cagcuacaac      660

gacaacuuca ucaaggagau ccucaaggug gccuucuucc agcggccgcu gaaggacuuc      720

uccuaccugg ugggcgccug caccuucuuc gaggacgaga agcgcgccug caagaacagc      780

uacuccgcgu gggaguucgu cgcccucacc aagaucauca acgagcucaa gagccuggag      840

aaggaguccg gcgagcucgu gagcucccag aucaucaacg agauccugaa ccacauccug      900

gacaagggga gcaucacgua caagaaguuc cgggaguaca ucaagcucca cgagagcaug      960

aaguucaagu cccugaagua cgacaaggac aacguggaga gcaccaagcu gaucgaguuc     1020

cgcaagcucg ucgaguucaa gaaggcccug ggcgagcacu cccugagccg ggaggagcuc     1080

gaccagaucg ccaccuacau cacccugauc aaggacaacg agaagcugaa gaucacgcuc     1140

gagaaguacu cccugaacaa cgagcagauc aagaaccugc ucgagaucga cuucaacgac     1200

cacaucaacc ugagcuucaa ggcccugaac cucauccugc cccugaugaa ggaggggaag     1260

cgcuacgacg aggcgugcaa gcugcucaac cugaagacca aguccaacaa ccagaaguuc     1320

gacuuccugc ccgccuucug cgacagcauc uucgcccagg agcucaccaa ccccaucgug     1380

aaccgcgcca ucuccgagua ccggaaggug cugaacgccc ugcucaagaa guacggcaag     1440

augcacaaga uccacaucga gcuggcgcgc gacaucggcc ugagcaagaa gcuccgggcc     1500

aagaucgaga aggagcagaa ggagaacuac gagaacaaca ucugggcccu gaacgagugc     1560

gagaaguucg ggcugaaggc caacgccaag aacauccuca agcugaagcu guggaaggag     1620

cagaaggagu ucugcaucua cuccggcaag aagaucagca ucgagcaccu ccgcgacgag     1680

aaggcgcugg aggucgacca caucuacccg uacucccgga gcuucgacga cuccuuccug     1740

aacaaggugc ucguguucac caaggagaac caggagaagc ugaacaagac gcccuucgag     1800

gccuucgggg ccaacgagga gcgcuggagc aagauccagg cccuggcgca gaaccucccc     1860

uacaagaaga agaacaagau ccuggacgag gccuucaagg gcaagcagca gcaggacuuc     1920

aucucccgga accugaacga cacccgcuac aucagcaccc ucaucgucaa guacaccaag     1980

gaguaccugg acuuccugcc ccucgacgag aaggaggacg ugaaccugaa guccggggag     2040

aagggcagca agauccacgu gcagacgauc aacggcaugc ugaccagcgu ccuccggcac     2100

accugggggu ucgcccagaa ggaccgcaac aaccaccucc accacgcccu ggacgccacc     2160

aucguggcgu acuccacgaa cgccaucauc aaggccuuca gcgacuucaa gaaggagcag     2220

gagcugcuga aggccaagcu guacgccaag gagcucaccu ccgacagcua caagcaccag     2280

gcgaaguucu ucgagccguu cgagggcuuc cgggagcaga uccugaacca gaucaacaag     2340

cuguucgugu ccaagccgcc ccgcaagcgg gcccgcgggg cccuccacaa ggagaccuuc     2400

uacagcaagg acgagaugau caagaaguac aacucccagg agggcgucga gaucgcccug     2460

aacugcggga agauccggaa gaucggcacc aaguacgugg agaacgacac gauggugcgc     2520

aucgacaucu ucaagaagca gaacaaguuc uacgccaucc ccaucuacac cauggacuuc     2580

gcgcugggcg uccuccccaa caagaucgug aucaucggga aggacaagaa gggcaacccc     2640

aagcaguggc aggagaucga cgagagcuac gaguucugcu ucucccugca caaggacgac     2700

cugguccuca uccagaagaa ggacaugcag gagcccgagu ucgccuacua caacggguuc     2760

gacaucagca acuccagcau cugcguggag aagcacgaca acaaguucga gaaccugacc     2820

gacaaccagc ggcugcuguu caccaacgcc gagggcaacg ugaaggccaa gaagaucggg     2880

auccagggcc ugcgcaucuu cgagaaguac aucaucacgc cccugggcga gaagaucaag     2940

gcggacuuca agccgcggga ggacaucgcc cucaagaccu ccaagaagca cgggcuguga     3000


<210>  1424
<211>  3003
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1424
augaagaucc ugggcuucga caucgggauc aacagcaucg gcugggccuu cguggagaac       60

gacgagcuca aggacugcgg gguccgcauc uucaccaagg ccgagaaccc caagaacaag      120

gagucccugg cgcugccgcg gcgcaacgcc cggagcuccc gccggcgccu caagcggcgc      180

aaggcccggc ugaucgccau caagcgcauc cuggccaagg agcucaagcu gaacuucaag      240

gacuacgugg cggccgacgg cgagcugccc aaggccuacg aggggagccu cgccuccgug      300

uacgagcugc gguacaaggc gcugacccag aagcucgaga cgaaggaccu ggcccgcguc      360

auccugcaca ucgccaagca ccggggcuac augaacaaga acgagaagaa guccaacgac      420

gccaagaagg gcaagauccu cagcgcccug aagaacaacg cgcugaagcu cgagaacuac      480

cagagcgugg gggaguacuu cuacaaggag uucuuccaga aguacaagga gaacaccaag      540

aacuucauca agauccgcaa caccaaggac aacuacaaca acugcgugcu guccagcgac      600

cuggagaagg agcucaaguu cauccuggag aagcagaagg aguucggcua caaguacucc      660

gagggguuca ucaacgagau ccugaagguc gccuucuucc agcggccccu caaggacuuc      720

agccaccugg ugggcgccug caccuucuuc gaggaggaga accgcgccug caagaacucc      780

uacagcgccu gggaguucgu ggcgcugacg aagaucauca acgagaucaa gucccucgag      840

aagaucagcg gggagaucgu cuccacccag accaucaacg aggugcugaa ccugauccuc      900

gacaagggca gcaucaccua caagaaguuc cgguccugca ucaaccugca cgagagcauc      960

agcuucaagu cccugaagua cgacaaggag aacgccgaga acgccaagcu caucgacuuc     1020

cgcaagcugg uggaguucaa gaaggcccug ggcguccaca gccucucccg gcaggagcug     1080

gaccagauca gcacgcacgu gacccugauc aaggacaacg ugaagcucaa gaccguccug     1140

gagaaguaca accuguccaa cgagcagauc aacaaccucc uggagaucga guucaacgac     1200

uacaucaacc ugagcuucaa ggcccucggg augauccugc cccugaugcg cgagggcaag     1260

cgguacgacg aggcgugcga gaucaccaac cucaagccga agacggugga cgagaagaag     1320

gacuuccugc ccgccuucug cgacuccauc uucgcccacg agcugagcaa ccccgugguc     1380

aaccgcgcca ucuccgagua ccgcaaggug cugaacgccc uccugaagaa guacgacaag     1440

gugcacaaga uccaccugga gcucgcgcgg gacgucgggc ugagcaagaa ggcccgcgag     1500

aagaucgaga aggagcagaa ggagaaccag gccgugaacg ccugggcgcu gaaggagugc     1560

gagaacaucg gccucaaggc cuccgccaag aacauccuga agcugaagcu cuggaaggag     1620

cagaaggaga ucugcaucua cagcggcaac aagaucucca ucgagcaccu gaaggacgag     1680

aaggcccugg agguggacca caucuacccc uacagccggu ccuucgacga cagcuucaug     1740

aacaaggucc ucguguucac caaggagaac caggagaagc ugaacaagac cccguucgag     1800

gccuucggga agaacaucga gaaguggucc aagauccaga cccuggcgca gaaccucccc     1860

uacaagaaga agaacaagau ccuggacgag aacuucaagg acaagcagca ggaggacuuc     1920

aucagccgca accugaacga cacgcgguac aucgccaccc ucaucaccaa guacaccaag     1980

gaguaccuga acuuccugcc ccucaacgag aacgagaaca cgaaccugaa guccggcgag     2040

aaggggagca agauccacgu ccagaccgug aacggcaugc ugaccagcgu gcuccgccac     2100

accugggggu ucgacaagaa ggaccggaac aaccaccucc accacgcccu ggacgccauc     2160

aucgucgccu acuccacgaa cagcaucauc aaggcguucu ccgacuucaa gaagaaccag     2220

gagcugcuga aggcccgcuu cuacgccaag gagcugacca gcgacaacua caagcaccag     2280

gugaaguucu ucgagcccuu caagggcuuc cgggagaaga uccucuccaa gaucgacgag     2340

aucuucguga gcaagccgcc ccgcaagcgg gcccgcggcg cccugcacaa ggagaccuuc     2400

uacuccgagg acgagaugau caagaaguac aacagcaagg aggggcugca gaucgcgcuc     2460

uccugcggcc ggguccgcaa gaucgggacc aaguacgugg agaacgacac gauggugcgg     2520

gucgacaucu ucaagaagca gaacaaguuc uacgccaucc ccaucuacgc cauggacuuc     2580

gcccugggca uccugcccaa caagaucgug guggucggga aggacaagaa caacaacccg     2640

aagcaguggc agaccaucga cgagagcuac gaguucugcu ucucccucua caagaacgac     2700

cugaucaugc ugcagaagaa ggacaugcag gaggcggagu ucgccuacua caacggcuuc     2760

gacaucagca ccuccagcau cugcguggag aagcacgacu ccaaguucga gaaccucacc     2820

agcaaccaga agcugcuguu cuccaacgcc aaggagggca gcgugaaggu cgagaaccuc     2880

gggauccaga accugaagau cuucgagaag uacaucauca cgccccuggg cgacaagauc     2940

aaggccgacu uccagccccg cgagaacauc agccucaaga ccuccaagaa gaacgggcug     3000

uga                                                                   3003


<210>  1425
<211>  3003
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1425
auggcccgcg cccagcggcg ccugaucagc cggcgcgcga accggaugaa caagcuccgc       60

cggcugcuga agcaggagaa cgugcuccag gccaacgacu acgacgagaa gggccugauc      120

aucgggcugc ccaaccaggc cugggagcuc cgcgcccagg gccuggaccg gaagcuggag      180

ccgaaggagu gggccgcggu ccucaucaac cuggugaagc accgcgggua ccugucccag      240

cggaagaacg agagccagac cgccgacaag gagcucggcg cccugcuguc cgggaugaac      300

cacaaccacc agcuccugca gagccagucc aaccaguacc gcacccccgc cgagcuggcg      360

aucaagcagu ucgccacgca ggacggccac auccggaacc agcgcggcgc cuacacccac      420

accuucaacc ggcucgaccu gcagaacgag cugaaccagc uguucgacgc ccagacccgc      480

cuggggaacc cccacgugag cggcucccug aagacggcca ucaacgaccu ccugaugaac      540

cagaaggcgg cccugagcgg ggacgccauc cucgagaugc ugggccggug caccuucgag      600

cccuccgagu acaaggccgc caagaacacc uacagcgcgg agcgcuucgu cuggcugggg      660

aagcucacca accugcggau ccaggacaac ggccuggagu ccgcccucag ccgcgagcag      720

cgggagcugc ugcucaacga gccguacaac aagacgaagc ugaccuacca gcagguccgc      780

aagcugcucc gccugcccga gaccgccuuc uucaagggcc ugcgguacgg gaaggacaac      840

gccgaguccg ccaccuucau ggagaugaag uccuaccacg cgauccgcaa gagccucgag      900

aacaagagcc ugaaguccga gugggagaag cugaagacgc agcccaagcu ccuggaccug      960

aucggcaccg ccuucagcau cuacaagacc gacgacgaga ucgccgggug ccucaagaac     1020

cagcugcccg agaacgugau caucgcccug caggagcagc ugaacuucuc caacuucaug     1080

gagcucagcc ugaaggcccu ggagaagcuc cugccgcuga uggagcaggg ccuccgguac     1140

gacgaggcgu gcacccagau cuacggcaac cgccacgaga agaucgccga caagacggag     1200

aaguuccugc ccaacauccc cgccgacaag cugcggaacc ccguggugcu ccgcacccug     1260

acccaggccc ggaaggugau caacgcgauc auccgccggu acgggagccc ggcccgcguc     1320

cacaucgaga ccggccggga gguggggaag uccuucagcg accgcgacaa gcugaagaag     1380

cagcaggagg agaaccagga gcagcggcgc cgggccaucg acgaguggaa gcaguucuuc     1440

cccaacgccg cccacgagcc caaggcggug gacauccuca agcugcgccu guaccagcuc     1500

cagcagggca agugccugua cuccgggcug cccaucgacg uccggcgccu cccggagaag     1560

ggcuacgugg agaucgacca cgcccugccc uucagccgga cgugggacga cuccuucaac     1620

aacaagaucc uggugcucgc cagcgagaac cagaacaagg gcaaccagac ccccuacgag     1680

uaccugcgcg gggccgacaa cuccgagagc uggcgccugu ucgucgagaa cgugcagaac     1740

ugccaguucu cccccaacaa gaagcagaag aucauggcca agcagcucga cgagaagggc     1800

uuccuggagc ggaaccugaa cgacacccgc uacgugagcc gguuccucug ccaguucauc     1860

uccgagcacc uccaccugac cgggaagggc aagaagcgcg ucuucgcgag caacgggcag     1920

aucacggccc uccugcgggg ccgcuggggc cuguccaaga accgggagga gaacgaccgc     1980

caccacgccg uggacgccgu ggucguggcc ugcagcaccg uggcgaugca gcagaagauc     2040

acccaguucg uccgguacca gaaggggaac guguucaccg gcgagcgcau cgacaaggag     2100

acgggggagg ucaucuccau ccacuucccg acccccuggg agcacuuccg ggaggaggug     2160

augauccgcg uguucgacga caacccccuc cggacccugc ccgagaagcu gccgaaccgc     2220

cccgaggccc uccacgagua cgucaugccc cuguucguga gccggaugcc cacccgcaag     2280

augacgggcc aggggcacaa ggagaccauc aaguccgcca agcggcugga cgagaagauc     2340

agcgugcucc gcgucccgcu gacccagcug aagcucaacg accuggagaa cauggugaac     2400

cgggagcgcg agcccgcccu guacgaggcg cucaaggccc ggcuggagca guucaagaac     2460

gaccccgcca aggccuucgc cgagccguuc uucaagcagg gcggccagca ggucaaggcg     2520

gugcgcgugg agaucgugca gaaguccggg augcugcucc gcaagaucaa cggcgucgcc     2580

gacaacggga gcauggugcg gguggacguc uucgagaagu ccggcaaguu cuaccuggug     2640

cccaucuacg ccuggcaggu cgccgagggc auccugccca acuacgccgu gguggcgcac     2700

aaggacgaga gcgacuggau ccucauggac gagagcuacc acuucaaguu cgcccuguac     2760

aagaacgacc ugcucgaggu gcagaccaag aagggcggga ucauggggua cuucgccucc     2820

cuggaccgcg ccacgggcgc caucaccauc cgggagcacg accgcgacaa gaagaagugc     2880

aaggacgggg agcaccggag caucggcguc aagauggcgu ccagcuucaa gaagcuccag     2940

guggacgagc ugggcaagaa cauccacgag acccgcuccc ggaagcgccc caacaucggg     3000

uga                                                                   3003


<210>  1426
<211>  3012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1426
augcgcaucc ugggcuucga caucgggauc aacagcaucg gcugggccuu cguggagaac       60

gacgagcuca aggacugcgg gguccggauc uucaccaagg ccgagaaccc caagaacaag      120

gagucccugg cgcugccgcg ccggaacgcc cgcagcuccc ggcgccggcu caagcgccgg      180

aaggcccgcc ugaucgccau caagcggauc cuggccaagg agcucaagcu gaacuacaag      240

gacuacgugg cggccgacgg cgagcugccc aaggccuacg aggggagccu cgccuccgug      300

uacgagcugc gcuacaaggc gcugacccag aaccucgaga ccaaggaccu ggcccggguc      360

auccugcaca ucgccaagca ccgcggcuac augaacaaga acgagaagaa guccaacgac      420

gccaagaagg gcaagauccu cagcgcccug aagaacaacg cgcugaagcu cgagaacuac      480

cagagcgugg gggaguacuu cuacaaggag uucuuccaga aguacaagaa gaacaccaag      540

aacuucauca agauccggaa caccaaggac aacuacaaca acugcgugcu guccagcgac      600

cuggagaagg agcucaagcu gauccuggag aagcagaagg aguucggcua caacuacucc      660

gaggacuuca ucaacgagau ccucaagguc gccuucuucc agcgcccccu gaaggacuuc      720

agccaccugg ugggggccug caccuucuuc gaggaggaga agcgggccug caagaacucc      780

uacagcgccu gggaguucgu ggcgcucacg aagaucauca acgagaucaa gucccuggag      840

aagaucagcg gcgagaucgu ccccacccag accaucaacg aggugcugaa ccucauccug      900

gacaaggggu ccaucaccua caagaaguuc cgcagcugca ucaaccugca cgaguccauc      960

agcuucaaga gccucaagua cgacaaggag aacgccgaga acgccaagcu gaucgacuuc     1020

cggaagcugg uggaguucaa gaaggcccuc ggcguccacu cccugagccg ccaggagcug     1080

gaccagaucu ccacgcacau cacccucauc aaggacaacg ugaagcugaa gaccgugcug     1140

gagaaguaca accucagcaa cgagcagauc aacaaccugc uggagaucga guucaacgac     1200

uacaucaacc ucuccuucaa ggcccugggc augauccugc cgcucaugcg ggaggggaag     1260

cgcuacgacg aggcgugcga gaucgccaac cugaagccca agaccgucga cgagaagaag     1320

gacuuccugc ccgccuucug cgacagcauc uucgcccacg agcuguccaa ccccguggug     1380

aaccgcgcca ucagcgagua ccggaagguc cucaacgcgc ugcugaagaa guacggcaag     1440

gugcacaaga uccaccucga gcuggcccgc gacguggggc uguccaagaa ggcccgggag     1500

aagaucgaga aggagcagaa ggagaaccag gccgucaacg cgugggcccu caaggagugc     1560

gagaacaucg gccugaaggc cagcgccaag aacauccuga agcucaagcu guggaaggag     1620

cagaaggaga ucugcaucua cuccggcaac aagaucagca ucgagcaccu gaaggacgag     1680

aaggcccucg agguggacca caucuacccg uacucccgca gcuucgacga cuccuucauc     1740

aacaaggugc uggucuucac gaaggagaac caggagaagc ugaacaagac ccccuucgag     1800

gcguucggga agaacaucga gaaguggagc aagauccaga cccucgccca gaaccugccc     1860

uacaagaaga agaacaagau ccuggacgag aacuucaagg acaagcagca ggaggacuuc     1920

aucucccgga accucaacga cacccgcuac aucgccacgc ugaucgccaa guacaccaag     1980

gaguaccuga acuuccuccu gcugagcgag aacgagaacg ccaaccucaa guccggcgag     2040

aaggggagca agauccacgu gcagaccauc agcggcaugc ugaccuccgu ccugcggcac     2100

acgugggggu ucgacaagaa ggaccgcaac aaccaccucc accacgcgcu ggacgccauc     2160

aucguggccu acagcaccaa cuccaucauc aaggccuuca gcgacuuccg gaagaaccag     2220

gagcugcuca aggcccgcuu cuacgcgaag gagcugaccu ccgacaacua caagcaccag     2280

gugaaguucu ucgagcccuu caagagcuuc cgggagaaga uccuguccaa gaucgacgag     2340

aucuucguca gcaagccgcc ccgcaagcgg gcccgccggg cccuccacaa ggacaccuuc     2400

cacuccgaga acaagaucau cgacaagugc agcuacaacu ccaaggaggg ccugcagauc     2460

gcccugagcu gcggccgcgu gcggaagauc gggacgaagu acguggagaa cgacaccauc     2520

guccgcgugg acaucuucaa gaagcagaac aaguucuacg cgauccccau cuacgccaug     2580

gacuucgccc ucggcauccu gcccaacaag aucgugauca ccgggaagga caagaacaac     2640

aacccgaagc aguggcagac caucgacgag uccuacgagu ucugcuucag ccuguacaag     2700

aacgaccuca uccugcugca gaagaagaac augcaggagc ccgaguucgc cuacuacaac     2760

gacuucucca ucagcacguc cagcaucugc gucgagaagc acgacaacaa guucgagaac     2820

cucaccagca accagaagcu gcuguucucc aacgccaagg agggcagcgu gaagguggag     2880

ucccucggga uccagaaccu gaaggucuuc gagaaguaca ucaucacgcc ccugggcgac     2940

aagaucaagg cggacuucca gccccgggag aacaucagcc ucaagaccuc caagaaguac     3000

ggccugcgcu ga                                                         3012


<210>  1427
<211>  3012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1427
augaucgacg agagccugac cuucggcauc gaccucggga ucggcuccug cgggugggcc       60

gugcugcgcc ggcccagcgc cuucggccgc aaggggguca ucgagggcau gggcuccugg      120

ugcuucgacg ugccggagac cagcaaggag cggacgccca ccaaccagau ccgccggucc      180

aaccgccugc uccggcgcgu gauccggcgc cggcgcaacc ggauggcggc cauccgccgg      240

cugcugcacg ccgccgggcu ccugcccagc accgacuccg acgcccugaa gcgccccggc      300

cacgacccgu gggagcuccg ggcgcgcggg cuggacaagc cccugaagcc cgucgaguuc      360

gccguggugc ucggccacau cgccaagcgg cgcggguuca agagcgccgc gaagcggaag      420

gccaccaaca ucuccagcga cgacaagaag augcugacgg cccuggaggc cacccgcgag      480

cgccucggcc gguaccgcac cgucggcgag auguucgccc gggaccccga cuucgcgucc      540

cgccggcgca accgggaggg gaaguacgac cgcaccacgg cccgggacga ccuggagcac      600

gaggugcacg cccuguucgc cgcccagcgc cggcucggcc agggguucgc gagcccggag      660

cuggaggagg ccuucaccgc cuccgccuuc caccagcgcc ccaugcagga cagcgagcgg      720

cuggugggcu ucugccccuu cgagcgcacc gagaagcggg ccgcgaagcu cacccccucc      780

uucgagcgcu uccggcugcu ggcccgccuc cugaaccugc ggaucacgac cccggacggc      840

gagcgccccc ucaccgucga cgagaucgcc cuggugaccc gcgaccuggg gaagacggcc      900

aagcucagca ucaagcgggu gcgcacccug aucggccugg aggacaacca gcgguucacc      960

accauccgcc ccgaggacga ggaccgggac aucgucgccc gcaccggggg cgcgaugacg     1020

gggaccgcca cccuccggaa ggcccugggc gaggcccugu ggacggacau gcaggagcgc     1080

cccgagcagc ucgacgcgau cguccaggug cuguccuucu ucgaggccaa cgagaccauc     1140

accgagaagc ugcgggagau cggccucacc cuggccgugc uggacgugcu ccugacggcc     1200

cuggacgccg ggguguucgc gaaguucaag ggcgccgccc acaucagcac caaggccgcc     1260

cgcaaccucc ugccgcaccu ggagcagggg cggcgcuacg acgaggcgug caccauggcc     1320

ggcuacgacc acgccgccuc ccggcucagc caccacgggc agaucgucgc caagacccag     1380

uucaacgcgc uggugacgga gaucggcgag agcaucgcca accccaucgc ccgcaaggcc     1440

cugaucgagg gccugaagca gaucugggcg augcggaacc acugggggcu ccccggcucc     1500

auccacgugg agcuggcccg cgacgucggg aacagcaucg agaagcggcg cgagaucgag     1560

aagcacaucg agaagaacac cgcccugcgg gcccgcgagc ggcgcgaggu gcacgaccuc     1620

cuggaccugg aggacgugaa cggcgacacc cuccugcgcu accggcugug gaaggagcag     1680

ggcgggaagu gccucuacac cggcaaggcc auccacaucc gccagaucgc ggccacggac     1740

aacuccgucc agguggacca cauccugccc uggagccggu ucggggacga cuccuucaac     1800

aacaagaccc ugugccucgc cagcgccaac cagcagaaga agcgcuccac cccguacgag     1860

uggcugagcg gccagaccgg ggacgccugg aacgcguucg uccagcggau cgagaccaac     1920

aaggagcugc gcggcuucaa gaagcggaac uaccuccuga agaacgccaa ggaggccgag     1980

gagaaguucc gcucccggaa ccugaacgac acgcgcuacg ccgcccgccu cuucgcggag     2040

gccgugaagc ugcuguacgc cuucggcgag cggcaggaga agggcgggaa ccggcgcgug     2100

uucacccggc ccggggcccu caccgcggcc cugcgccagg ccuggggcgu cgagagccug     2160

aagaagcagg acgggaagcg gaucaacgac gaccgccacc acgcccucga cgcccugacc     2220

guggcggccg uggacgaggc cgagauccag cggcugacga aguccuucca cgagugggag     2280

cagcagggcc ucggccgccc ccugcgccgg gucgagcccc cgugggagag cuuccgcgcc     2340

gacguggagg ccaccuaccc cgagguguuc gucgcgcggc ccgagcgccg gcgcgcccgg     2400

ggcgaggggc acgccgccac cauccgccag gugaaggagc gggagugcac ccccaucgug     2460

uucgagcgca aggccgucuc cagccugaag gaggcggacc ucgagcggau caaggacggg     2520

gagcgcaacg aggccaucgu ggaggccauc cgguccugga ucgccacggg ccgcccggcc     2580

gacgcgccgc cccggagccc ccgcggggac aucaucacca agauccggcu ggccaccacc     2640

aucaaggccg ccgugcccgu ccgcggcggc acggcggggc ggggcgagau ggugcgcgcc     2700

gacguguucu ccaagcccaa ccggcgcggg aaggacgagu gguaccuggu ccccguguac     2760

ccgcaccaga ucaugaaccg caaggccugg cccaagccgc ccaugcggag caucguggcc     2820

aacaaggacg aggacgagug gaccgagguc ggccccgagc accaguuccg cuucucccuc     2880

uacccccgga gcaacaucga gaucauccgc cccagcgggg aggugaucga gggcuacuuc     2940

gugggccugc accggaacac cggggcccug auccccaccc cggucggccc cgacuccuac     3000

gugaucgcgu ga                                                         3012


<210>  1428
<211>  3024
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1428
augaaggugc ugggcuucga caucgggauc aacagcaucg gcugggccuu cgucgagaac       60

aacgagcuca aggacugcgg ggugcgccug uucaccaagg ccgagaaccc caagaccaag      120

gagucccugg cgcucccgcg gcgcaacgcc cggagcuccc gcgugcggcu gcgccggcgc      180

cggagccgcc ugcucucccu gaagcacauc aucagcaagg aguucggccu gcacuacgag      240

gacuacaucg cccucgacgg gaaccugccc aaggccuaca cgggcaagau cgaguccauc      300

uacaagcugc gguacgaggc ccucagccag gcgaucucca aggaggaccu ggcccgcguc      360

auccugcaca ucgccaagca ccggggcuac augaacaaga accagaagac cagccaggac      420

uccgagaagg gggccauccu cagcgcgauc aaggagaacu ccaacaagau caagcacuac      480

cagaccgccg gcgaguacuu cuacaaggag cuguucgaga accugcgcga cgggaccaag      540

gaguucaaga acauccggaa caaggccaug aagaacgaga agacgggcaa cauggagagc      600

accuacuaca acaccauccu cgccuccgac cuggagaagg agcugaagcu cauccuggag      660

aagcagcagg ccuaccacaa cuacagcgac accuucauca aggaggugcu ggagaucgcg      720

uucuaccagc gcccccucaa ggacuucucc caccuggugg ggagcugcac guucuacgag      780

aacgagaagc gggccugcaa gaacuccuac agcgccuggg aguucaucgc ccugggcaag      840

aucaucaaca cccucgagag caucaagaag gacaccggcg agaucuucuc caacgagauc      900

aucacccaga uccugaagga gguccuggac aaggggagcc ucuccuacac gaagcugcgc      960

aagaugauca accuggacga gaaggugaag uucaagggcc ucaaguacga caaggagggg     1020

guggagaaga ccaaccugau cgaguucaag aagcugaagg aguucaagaa ggcccucggc     1080

gagcacaacc uggagcggaa gacccuggac gagaucgcga cccacaucac gcucaccaag     1140

gacgagaacc agcugaagaa gcagcuggag caguacgccc ucagccagaa ccagaucgac     1200

gcccuggccg agcuggacuu caacgaccac aucaaccucu ccuucaaggc ccuguacgag     1260

auccugcccc ucauggagaa gggcaagcgc uacgacgagg cgugcaacga gcuggggcug     1320

aaggccaaga ccagcaacca gaaguccgag uuccugccgg ccuucugcga cagcaucuuc     1380

gcccaggagc ucaccaaccc caucgucaac cgggccaucu ccgaguaccg caaggugcug     1440

aaccgccugc ucaagaagua cggcaagaug cacaagaucc acaucgagcu ggcgcgggac     1500

gccgggcuga gcaaggaggc ccgcaacaag aucgagaagg agcagaagga gaacaaggac     1560

aucaacgaca aggccgagaa gacgugcaag gaguucggcc ucaagcccaa cgcgaagaac     1620

auccugaaga ucaagcugug gcgggagcag ggggagaucu gcgccuacuc cggcaagaag     1680

aucaccaucg acgaccucaa ggaggacaag gcccuggagg uggaccacau cuaccccuac     1740

agccgcuccu acgacgacag cuacaacaac aagguccugg uguucaucaa ggagaaccag     1800

cucaagcuga accagacccc guacgaggcc uucggcucca accaggagaa guggagcaag     1860

auccaggugc uggcccagaa gcuccccuac aagaagaaga accggauccu ggacgagaac     1920

uucaagaaca aggaccagau cgaguucauc ucccgcaacc ugaacgacac ccgguacgcg     1980

acgagccuca ucgccaagua caccaaggag uaccuggagu uccugccccu cuccgacgac     2040

gaggacauca gccucaagag cggggagaag ggcuccaaga cccacgucca gaccaugaac     2100

gggaugcuga cgagcgugcu ccgccacgcc uggggcuucu cccagaagga ccggaacaac     2160

caccuccacc acgcccugga cgccaucauc aucgcguaca gcaccaacuc caucaucaag     2220

gccuucagcg acuucaagaa gaaccaggag auccugaagg ccaagcugua cgccaagaag     2280

cugaccuccg aggaguacaa gaaccagagc aaguucaccg gguucgagaa guaccgcucc     2340

aagauccucg agaagcugga cagcaucuuc guguccaagc ccccgaacaa gaacacgaag     2400

ggcgcccugc acgagcagac cuucuacagc uaccaggaca uccucgagga guacaagacc     2460

caggagggcc ugaagcgggc gcuggagugc gggaaggucc gcaagaucgg caccaaguac     2520

guggugaacg accggauggu ccgccucgac aucuucaaga aggacaacaa guucuacggg     2580

auccccaucu acacgaugga cuucgcccug ggcgugcugc ccaacaaggu ggucaccauc     2640

gggaccgaca aggacaagau caagaaggag uggaagacca ucgacgagac cuacgaguuc     2700

ugcuucuccc ucuacaaggg cgaccuggug cugauccaga agaaggagau gcaggagagc     2760

gaguucgccu acuacaacgg cuucgacauc uccagcgccu ccaucagccu cgagaagcac     2820

gacaacaagu ucgagaaccu gacggagaac cagaagcugc uguuccccaa ggcgaaggag     2880

ggggacgucg aggugcgggg caucgggauc cagggccuga agguguucaa gaaguacaug     2940

gucaccccgc ugggcgagau ccaggagacc cgcaccgagg cccggcagaa cauccagccc     3000

cgcaagaagc acgggcuccg guga                                            3024


<210>  1429
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1429
augagcgacu ccagcuacgu gcugggccuc gaccugggga ccaacuccau cggcugggcc       60

ugcguccgcc ugaacaaggg gaagcccgac ggccuccugg cccagggggu gcggaucuuc      120

gaggcgggcc uggagaucga cgccuacggc cgcgggaccc cgcucaacac ggagcggcgc      180

aacgcccggc agcgccggcg ccugcacgac cggcgcaagc ggcgcauccu gaaggugcgg      240

cgcauccucc aggaggccgg caugcugccc gccaacaagc ccgaccacaa ggacgcggag      300

uggaagaugc ugcucgcccg gaaccccuac cgccugcggg ccaaggcccu ggacgagcag      360

aucgaccuca aggagaucgg gcugugcauc uaccaguucg cgcaccgccg gggcuuccug      420

agcaacucca agauccucaa gaaggacgac gaccgcggga agcugaagga cgccaccaag      480

gccuucgccg aggacaugga gcagaagggc gcccggaccu ggggcgagua ccugagccac      540

cucgacccgc acgagcagcg cauccgcacc aacuacacgc ugcgggagug guacaccucc      600

gaguucgacc ugaucuggga gaagcagcag agcuucuacc cccacguccu caccgacgac      660

cuguacaagc gcgugaagga ggcgcuguuc uaccagcggc cccucaaguc ccaggcccac      720

cuccuggggc agugcgagcu ggagcccggc cgcaagcggg ccccguucgc ccugcugaug      780

gcccaggagu uccgccuccu gcagagccug aaccaccucc ggguggucuc cagcaccggg      840

gacgagcgcc ugcugacgga cgaggagcgg aagcaggcgc ucgcccugcu gcgcucccgg      900

ggcaaccaga aguucaccac ccuccgcaag gugcugaacc ugcggggcac caaguucaac      960

cucgagcgcg ggggcgagaa gacgauccag gggaacaaca ccgccgccgc ccugggcaac     1020

cuguucgggg aggcguggga ccacuucagc uccgagcaga agaaccagau cguggaggac     1080

cggcucacca ucggcgacac cgacgcccug auccgccgga ugaagaagac guggggccug     1140

gacagcguca ccgccgagau guucuccgag accgagcugg aggacgggua cugcggcuuc     1200

agccgcaagg cccucgagaa gcugcugccc cuccuggagc gggggcacgc ccugauggag     1260

gcggugcagg ccgccuaccc cgacuaccag aagaaccucc cccgcaccga cacgcugccg     1320

ccccuggaga accuccgcaa ccccaucgug cagcggaccc uguccgagcu gcgccggguc     1380

gugaacgcca ucaucaagga guacggcaag agcgacaaga uccgcaucga gcucgcgcgg     1440

gacaugaagc agaccgccaa gaaccgcgag aaguccuggg accggaugcg cgugcgggag     1500

cgccagcgga aggaggccgc caagaagauc cuggaggaga ccgggaaccc cgccccgcgc     1560

cggcacgaga ucgagaaggu ccugcucgcg gaggagugca acugggagug ccccuacacg     1620

ggcaagcgca ucugcaugaa cacccugcug ggcgacgcgc cccaguucga caucgagcac     1680

aucauccccu ucagccggag cuucgacaac uccuucagca acaagacccu cugcgacgug     1740

aacuacaacc gcaacgugaa ggggaacaag accccguacg aguccgccag cgagacggag     1800

cacgaggaga uggucugccg ggugaagcgc uucaccggcg acuacaagga gaucaagcug     1860

gaccgguuca acacccgcga gaccgacucc cgggccgacu ucaucgagga guucagcacg     1920

ucccagcuga acgacacccg cuacgccagc cgccucgccg gggaguaccu gggcaagcug     1980

uacggguccg aguggcggaa gcacauccag aucgccaagg gcggcaccac caaguaccuc     2040

cgcgacgccu gggaccugaa ccggauccug agcgacgggg gcacgaaguc ccgcgacgac     2100

caccggcacc acgccgugga cgccgucgug guggcgcuca ccacgcccca gaccgucaag     2160

gcccugagcg acgccaacca gcgcgccccc gagcaccggc gcuacgcguu cuccgacaug     2220

ccccugccgu gggagaccuu ccggcaggac guggagcagg ccaucaacag caucaccguc     2280

ucccacaagg ugugccgcaa ggccaacggg cagcuccaca aggagaccuu cuacggccug     2340

aucaaggacc ggaagaccgg cgacacccgc acggugcugc ggaagccccu ccacgagcug     2400

accgacaagg agaucaagcg cgccgagauc gucgacagcu ccauccggaa gcugaucgag     2460

cagaagcuca aggaccugga ccagccgccc aagaccgccu ucaaggaccg caacaaccac     2520

cccuucauca ucgacaagaa cgggaacaag cgguucaucc acaaggugcg cguguaccag     2580

gacgcgaagc ccgucaagau cgccgagaac cggcacguga agcugggcgg gaaccaccac     2640

cucgagaucu ucgaggugau cgacaagaag ggcaacccca ccuggaagug cgaggucauc     2700

agccuguucg acacgcgcca gcgcgugaag gagaagcggc cggugaucgu ccgccaggac     2760

cagaacggga acccccugaa guucagccuc uccaucggcg aggccgugca gcuggacugg     2820

aacggcgggc gggagaccgc cgugguccag aagaugagca agacccccaa caccguggag     2880

uacguguucc gcaagcacaa cgacgcccgg aaggcggccg acauccccua caaggagacg     2940

aucaccguca aguccgacag cgcccuguuc gccuccaacc uccagaagau cgugaucacc     3000

ccgaccggcc ugauccgccg ggccaacgac uga                                  3033


<210>  1430
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1430
augaagaacc gcaacgugau cggcaucgac gucgggacca acagcaucgg cugggcccug       60

guggaggaga acgagaacgg gcucccccag ucccugaagg ccgcgggcgu gcggaucuuc      120

caggaggccg ucgaggccaa gacccgcacg ccgaagaacc acaagcggcg ccaggcccgg      180

cuggcccgcc gggugcucgc gcgccgggcc cgccggaagc agcgccugca gaaccugcuc      240

cugaagcacg ggcugcuccc cggcggcgug accgaccccg uccagcggga ggccgccuuc      300

accacccugg gggaccccua ccagcugcgc gcgaagggcc ucaaccugcc gcuggggcuc      360

cacgaguucg gccgggugcu guuccagcug ggggcccgcc ggggcuuccu cagcaaccgc      420

aagacgcggu acggccaccu gaacaagcug gacgagcucg ccgaccugau cgaggccgag      480

gacgccgcgc ccaagaccgg ggacgccgag gagggcauca uccgcaagga gaucggggag      540

cugcggcagg ccauguccgc cgccggcagc cgcacccucg gcgaguaccu ggcggggcug      600

gccaaggagg agcgcaagcg gugccgccac accgagcggg ccaugcucga ggaggaguuc      660

gagcgccugu ggauggugca ggcccgguuc caccccgagc uguccgacga gcucaagguc      720

caggugcaca cggugaucuu cuuccagcgc ccccucaaga gcaaccgggc cucccugggc      780

aagugcagcc uggagccguc ccgccggaag gcggcccgcg ccuggcccga ggcccagcgg      840

uuccgccugc ugcaggaccu caacaaccug gagauccugg acccccggac cggggagcuc      900

cgccggcugc gcgacaccga gcgggccauc cugcucaacg cgcuggagca gcagaagucc      960

augaccugga acgccguccg caagaagcug ggccuccacc agggggagaa guucaaccug     1020

gagacgggcg ccaaggccga gaccggccug aaggggcucc agacccuggc gagccugcac     1080

gccauccugc agggcugguu cgacgagcuc ggggacaagc gggaccugcu gaucgaggac     1140

cucaucacca ucgaggacag cgccggccug uaccgccgcc ugcgggagca cuggggguuc     1200

gaccgcgaga cggccuuccg gcucgccacc cuggagcugg agcccggcua cgcgucccuc     1260

agccugaagg ccauccgcaa gcugcucccg cacaugggcg ccgggcaccg guacuccgag     1320

gcccgccagc aggucggcua cggguacgag accggccagc gggaccugca ggagcgccug     1380

ggggagccgc ccgacccccg gaaccccgug guggacaagg cccuccacga gcugcgccgg     1440

gugaucaacg cgcugaucca ccgcuucggc cggcccgacg ugguccgcgu ggagcucgcc     1500

cgggaccuga agcuggccaa gcgcgacaag gagcggcucg agaagcagaa ccgcgagaac     1560

cagcggcuga accgcaaggc cgaggccgcg cuggccgagc ucggccuggg gcagcccagc     1620

cgggacgacc ugaucaagua ccgccucugg gaggagucca agggccugug ccccuacacc     1680

gggcgcgaga ucggccugag cacccucuuc uccccggagg uggacgucga gcacaucauc     1740

cccuacagcc ggugccugga cgacagcuac augaacaaga cgcugugcau gaccgaggag     1800

aaccgcaugc agaagggcaa ccggaccccc ugggaggccu acgggggcga ccccgcccgc     1860

uacgagcaga uccuccagug cgcgcgguac uucccgaugg ccaagcgcaa gcgguuccug     1920

gagcaggggu ccgacuucgu ggaccaguuc auccagcgcc agcugaacga cacgcgguac     1980

aucgccaagc ucgccaagga guaccuggcc gcggugugca ccaacgucca ggugaccaag     2040

ggcgggacca cggccaugcu gcgccaccac uggggccuca accggcugcu gggcgaggac     2100

gacgggcaga aggagcgcgg cgaccaccgg caccacgccc ucgacgccgc cgucaucgcg     2160

gugaccagcc gcucccugua ccagcggcug gcccgcaucg cccaccagcg ggggcacgcc     2220

cccagccagc gcggcuucua caucgacccg cccuggcagg gguaccaggc cgaccucgcg     2280

cagacccugg ccggcaucgu ggucucccac gccaccaacc ggaagcugac gggcgcccuc     2340

cacgaggaga ccgcguacgg gcacaucccc cacgugggcu ucguguaccg caagacccug     2400

aacaaggacu ucaagcuggg ggacaucgag gccaucuacg accccagccu ccaggagcug     2460

gucaaggccc accuggagca ccacggcaac gaccccaagc gcgccuucgc cgaggggauc     2520

cagugcuacc acaaggacgg ccacaccccg aucaagcggg ugcgcauccg ggcgaagcag     2580

gucuccgaga agggcacguu cagcaucucc gaccgcgccg ggcgggccua caagcaccac     2640

aagaccggca gcaaccacca cguggagauc cuccgccacc gggccaccgg gaagaucgag     2700

cccguguacc ugaccaccaa ggaggccacg gagcgcgcgc ggcgcggcgg ggagcccgug     2760

auccagcggg accacggccc cgagugggag uucaucaucu cccugugcau caacgacaug     2820

guccgccucg gcgagggcgg ccagguggcc uacuuccggg ugcagaagau cgaggccacc     2880

aacaaccgcg ucacccugcg gagccaccug gccgccaccc ucgacgacga ggacaugaag     2940

gugacgaccu ccgugccgaa gcuggcgguc augcccgggu uccagaaggu gcuggugacc     3000

gccgucgggg aggugcucga gcugcacgac uga                                  3033


<210>  1431
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1431
augaagcgca uggccgccgu gggccugaug cccgcggacc cgaccgaggc caaggcccuc       60

gagcugcugg accccuacgc ccuccgggcc gcggggcugg accaggcccu gccccucacc      120

cacuucggcc gcgcccuguu ccaccugaac cagcggcgcg gguucaagag caaccggaag      180

acggacaagg gcgacaacga guccgggaag aucaaggacg ccaccaagcg ccucgacgag      240

gagauggcga ucaagaacgc ccggaccuac ggcgaguucc ugcacaugcg ccggaccagc      300

gccacggacc ccaagaacgu cccgaccgug cgcacccggc uguccaucgc ccgccgggac      360

aacgccgaga aggaggaggc gggcuacgac uucuaccccg accgccggca ccucgccgag      420

gaguucgaca agcuguggaa ggcccaggcc ggguacagcc ccgacgugcu gaccgacgag      480

cuccgcgacg agaucuccgu caucaucuuc caccagcggc cccugaagac gccggaggug      540

ggccugugcc ucuucagcgg gucccacggc gugcccaaga aggacaagcg cauccccagc      600

gcccaccccc ugaaccagcg gcgcauccug uucgagacca ucaacaaccu ccgggucgcg      660

gcccgcgggg agccggcccg cggccugacc cgggacgagc gcgacgccau cgugcacgcc      720

cuggacaaca aggcgcacac caagucccuc agcggcaugu ccaugaagcu gaaggcccug      780

gggaagguga ucaagcuccg gcccgagcag agcuucacgc ugcagaccgc caaccgcgag      840

accaucgccu gcgaccccgu ccgggccucc cugagccacc ccgaccgcuu cggcccgcgg      900

ugguccaugc ucgacgcgga cgcccagugg gacguggugc agcgcauccg ggccguccag      960

agcgaggccg agcacgacgc gcuggugaag uggcugaugg ccacccacgg gcucgaccgc     1020

gaccacgccg aguccacggc caaggcgccc cugcccgagg gcuacggccg gcuggggcuc     1080

accgccacca agcgcauccu ggccgcccug gaggccgacg ugauccccua cagcgacgcc     1140

gucaaggcgu gcggcuggca ccacuccgac gggcggaccg gcgaggugcu cacggagcug     1200

ccguacuacg ggcagauccu ggaccgccac gugauccccg gcaccuacaa cgagagcgac     1260

gacgacguca cccgguacgg ccgcaucacc aaccccacgg ugcacaucgg gcucaaccag     1320

cugcggcgcc uggugaacaa gaucaucacc gucuacggca agcccgacga gaucguggug     1380

gagcucgccc gggaccugaa gcugagcgag gaccagaagc gcgacgucca gcgcgacauc     1440

aagaagaaca ccgacgccgc caucgcccgc ggggcgaagc ucuccgagau gggcgugccg     1500

gacaccgggg ccaaccggau gguccugcgg cugugggaga gccugggcga cgacgugaug     1560

acgcgcaacu gccccuacuc cggcaagcgg aucagcgcca ccaugcuguu cgacgggucc     1620

ugcgacgugg accacauccu gcccuacagc cgcacccugg acgacggcuu cgccaaccgg     1680

acccucugcc ugaaggaguu caaccgccag aagacggaca agacccccug ggaggcgugg     1740

ggcgacaccc cggccugggc caccaucgag gccaaccuga agaaccuccc cgccaacaag     1800

gcguggcggu ucgcccccga cgccauggag cgcuucgagg gggagaacga cuucacgucc     1860

cgggcccuga aggacgccca guaccugagc cgcaucgcgc ggaccuaccu cgacgcccug     1920

uacgacgggg ccgacggcaa gucccacguc uggguggugc cgggccgccu gaccgagaug     1980

cuccggcgcc acugggggcu gaacagccug cuccccgaca aggacggcgc cgucaaggcc     2040

aagaaccgga ccgaccaccg ccaccacgcg aucgacgccg ccgugguggc cgcgaccgac     2100

cggucccuga uccagcgcau cagcaagaug gcccggaagg acgaggucaa cggggccgag     2160

gagguggccc gcuccgugcc cccgcccugg gagggcuucc gcacggacau cgagacccag     2220

cuggaccgga ucaucaucag ccaccgcgcc gaccacggcc ggaucgaccc cgcggcccgc     2280

aaggacgggc acgacuccac cagcggccag cuccacaacg acaccgccua cgggcugggc     2340

gaggggaacu ccgucgugag ccggaagccc cugcucuccc ucaagagcaa cgacaucgag     2400

gugacgacca agggcgccaa cauccgcgac gucgaccugc agcggcaccu cgagcgcauc     2460

acccggggcc uggacgggaa ggcccuggag gcggcccucg ccgaguucgc cgccaccgcg     2520

aagcugcccg acggcacgga caacccguac uacgggcugc gccgggugcg ccucgaggag     2580

acccugcagc agggcgcccg gguggagguc aaggaccgca acgggaagcc cuucaaggcc     2640

uacaagggcg acagcaacca cugcuacgag aucuggcgga ugcccgacgg caagguggug     2700

ccccagguca ucaccaccua cgaggcccac cagaucgacg uggagaagaa gccgcacccc     2760

gccgcgaagc gccugcuccg gaucuucaag cgcgacaugg uggccaucga gcaggacgac     2820

aaggccauca ucggguacgu ccaguccaug cacguggcca acggccuguu caucgcgccc     2880

cacacggagg ccaacgccga cgcccggagc cgcgacaagu ccgacagcuu ccgccugauc     2940

cagauguccg ccgggccccu cgugaaggcg aacgcccggc gcgucuacgu ggacgagaug     3000

ggccggcugc gcgacccggg ggcccccagc uga                                  3033


<210>  1432
<211>  3039
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1432
auggccaaca ugcgcuaccg gcuggcccuc gaccugggca gcaccucccu ggggugggcg       60

auggugcgcc ucgacgccca gaacgagacc ugcgccguca ucaaggccgg cgugcggauc      120

uucagcgacg ggcgcaaccc caaggacggc uccagccugg ccgugucccg gcgcgaggcg      180

cgggccaugc gccggcgccg ggaccgccug cucaagcgga agacgcgcau gauggccacc      240

cugaugaagc acggguucuu cccgcaggac gaggccgcgc ggcaggcccu ggucaacaag      300

gagcccuucg cccuccgcgc caagggccug gaccacgccc ugcuccccga ggaguucgcg      360

cgggcccugu uccacaucaa ccagcgccgg ggcuucaaga gcaaccgcaa gaccgacaag      420

aaggagaccg acuccagcgc ccugaagaag gccaucgggc agcuccggga ggccaugcag      480

gcguccggcu gccgcacggu gggggagugg cugugcgccc gccaccagca gggccugagc      540

gugcgggccc gcuaccggga gaaccgcauc accaaggacg acgggaagac caagaucgag      600

aaguccuacg accucuacau cgaccgggcc augaucgagg ccgaguucga cgcgcugugg      660

gccaagcaga gcgagcugaa ccccgagcug uuccacgaga ccgcccgcgc cgagcugaag      720

gacugccugc uccaccagcg gccgcugaag cccgucaagc ccggccgcug cacgcugcuc      780

cccgacgagg agcgggcccc gcuggcgcug cccucccagc agcgcuuccg gaucuaccag      840

gaggugaaca accuccgccu gcugcgggag ggccucaacg aggagccccu gacccugacc      900

cagcgcgacc agcucaucca ggcccuggag accaacccca agcggagcuu cacgcagauc      960

cagaagcugc ucggguucuc cggccaguuc aaccugcagg acgacaagcg caccgagcug     1020

aaggggaaca gcaccuccgc caugcucagc aagaaggagu ucuucggcuc ccaguggcug     1080

agcuucgacg agacccacca ggacgagauc gugaugcagc uggucagcga ggagaacgag     1140

gcccggcuca ucgcguggcu gcaggccgag acgggcgugg acgagaucac cgccgagcgc     1200

aucgccaaca ccccgcuggc cgagggguac ggcucccuca gcgcgaaggc ccuggagaag     1260

auccugcccg cccugcgggc cgacgugauc accuacgaca aggccguccu cgcggccggg     1320

uucgaccacc acucccacau cagccacgcc gccacgggcg agauccugcc cgaccugccc     1380

uacuacgggc aguaccucca gcgccacgug ggcuucggca ccgggaagcc ggaggacccc     1440

gccgagaagc gcuacggcaa gaucgcgaac cccaccgugc acaucgggcu gaaccagguc     1500

cggaccgugg ugaacgcccu gaucaagcgc uacggccacc ccuccgaggu cgugguggag     1560

cucgcccggg accugaagca gagccaggag cagcgcaagg aggagcaggc ccagcaggcg     1620

aagaaccagc ggcgcaacga gcggaugcgc caggacaucg ccgggcugcu caacacgucc     1680

accgagcggg uccgcgcccu ggaccugcag aagaugaucc ucugggagga gcugagcuuc     1740

aacguggccg accggcgcug cccguacucc ggcgugcaga ucagcgccca gaugcuguuc     1800

uccgaccagg ucgagaucga gcacauccuc cccuucagcc agacccugga cgacucccug     1860

aacaacaaga ccguggcgau gcggcaggcc aaccgcauca agggcaaccg gacgcccgcc     1920

gaggccgccg cggacuucgc cgcccagggg uggcccgucg aggacaugcu ccaccgcgcc     1980

agccugaugc cgaagggcaa gcgguaccgc uucgcccagg acgggauggc gcaguggcug     2040

cgggaggaca agggcuuccu cgcccgcgcc cugaacgaca cccggcaccu gucccgcgug     2100

gcccgcgagu accucagccu ggugugcccc caggggaccc gggucauccc cggccgcaug     2160

accgcgaugc ugcgggccaa guucggccuc aacgacaucc uggggcugaa cggcgagaag     2220

aaccgcaacg accaccggca ccacgccgug gacgccugcg ugaucgccgu cacggaccag     2280

uccaugcucc agaaguucgc gaaggccagc gccuccgccc gcgagaagca gcugaacaag     2340

cugguggaga gcaugccccu cccgugggac agcuaccggg accacgugca gcgcgccguc     2400

gagcggaucu ggguguccca caagcccgac cacgggcacg agggcgcgau gcacaacgac     2460

accgccuacg gccugcucgg gggcgggcac gugcaggucc gcaagcggau ggacggcgug     2520

cgcacccggg agaccgccgc ccucaaggug aucgaguucg ccgaccgcca ggcgcacccc     2580

cggcacgggc ugcugccgaa cggcgagccc augcccuaca agggcuacaa gggggacagc     2640

aacuacugca uggagaucgu ccgcaacgac aagggcaagu gggaggggga gguggugucc     2700

acguucgagg ccuaccagcu cguccggcag cacggccugg cccgccugcg gcaccccgcc     2760

cucagcgugu ccgggcgccc gcuggugaug cgccugcucc ggaacgacac ccugcgcguc     2820

auccacaacg gcgagcagcg gaccgugcgc aucugcaaga ugaccgccga gggcgugauc     2880

gcgcuggccg acaccacgga gagcaacguc gacgcccgga cccgcaccaa ggaccucucc     2940

uacaucuuca agaccgccgg gagccugcag aaguccaagg gccggcgcgu ggcgaucagc     3000

cccuccgggg agaugaagga ccccggcuuc uucgacuga                            3039


<210>  1433
<211>  3051
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1433
auggaggugu ucgucgacgu gagcgcccgc cagcugggca ucgaccucgg gaucgggacc       60

ugcggguggg cccugcuggc ggaggacucc cucaucgccc ugggcacccg gacguucgac      120

gugcccgaga ccgacaagga gcgcaucccg accaaccaga uccggcgcca ggcccggggg      180

augcgccggg ucaucgaccg ccggcgccag cggaugaacg ccgugcgccg gcugcucgcc      240

gaccacggcc ugcuggcgga cggcgggaag cucggccugg ccaccgugcg ccuggacccc      300

uggcaggccc gggccgaggg gcucgaccgc cugcugagcg cgggcgagcu cgccgucgcc      360

cuggggcaca ucgccaagca ccggggcuuc cgcuccaaca gcaagcggga ccgcggcgcc      420

aacgcgccca aggaguccag cgccaugcug ggggccaucg cccagacggg cgagcggcuc      480

cagcacuggc gcaccguggg ggagauguuc uggaaggacg aguucuacca gacccgccgg      540

cgcaaccggg acggcgacua cucccgcacc gugcagcggc uggaccugga gacggagguc      600

caccgccucc uggccgcgca gcggcgccug ggcaaccccc ucgccaccuu cgaccuggag      660

cagagcuuca ucgacguggc cuucuuccag cggccgcugg ccgacuccga cgacaaggug      720

gggcccugcc ccuucgagcc cagcgagaag cgcgccgcgc ggcacucccc gagcuucgag      780

cgcuuccggc uccugucccg ccuggccgcc cuccggcuga gcuccggcgc cggggagcgc      840

ccccugagcg acgccgaguu cgcggccgcc ugcgccgacu ucggccggcu ccagggguuc      900

uccuucaagc gccugcggca gcaccugaag cucgacccca gcauccgcuu cgcgggcauc      960

gcccccgacg aggagggcaa gcgcgacguc guggcccggu ccggggaggc caccccgggc     1020

agcgccgcgc ugcgccacug ccugggggac accguguggg acaacaugcu cggcacgcgg     1080

cccgcccugc uggacgccau cgccgccauc cucaccuucc gcgacgacgc ggaccggauc     1140

gccgaggggc uggccggccu gggcaucgag gagcccaucc ucgacgcccu gauggacggg     1200

guccgcaacg gcagcuucgg gaaguucaag ggccccgggc acaucuccgc caaggcgugc     1260

cgggcccugc ucccgcaccu ggcccgcggc cggguguaca gcgacgccug cgcggacgcc     1320

ggcuacgacc acucccgcca gaccgagacc aaccuggacg acauccggaa ccccguggcc     1380

cgcaaggccc ugggggaggc ccucaagcag aucaagauca ucauccacac gcacggccgg     1440

cccaccgcga uccacgucga gauggcccgc gacaucggca agagcgccga cgagcgggac     1500

gagaucaaga agggcaucga caagcgcaac uccgagaagg accgggcccg ccagcaguuc     1560

cacgagaugg ugggcacccc cgccgcgggg gccgaggacc ugcugcgguu cgagcucugg     1620

aaggagcaga acggcuggug ccuguacacc gggcgcgaga ucccgaucga cgcccuggug     1680

agcuccgaca accggaucca ggucgaccac auccuccccu ggagccgcuc cggcgacgac     1740

agcuacauca acaagacgcu gugcuucacc gaggccaacc agcacaagaa ggggaugacc     1800

cccugggagu ggcugcacga cgaccccgag cgcugggaga aguucgcccg gaccguggag     1860

ggcugcaaga cgaugaaggg ccgcaagaag cggaaccucc ugcugcgcga cuccagccgg     1920

cucgagaagg aguuccgcuc ccggaaccug aacgacaccc gcuacgcgug ccggcugcuc     1980

cuggggaucc ugaagcacac cuacccggac aucguggucg ccgcccgccc cggcccccuc     2040

accgaccggc ugcgccgggc cugggggcug cagagccuca agaagcacga ggacggcacg     2100

cgcaaggacg acgaccggca ccacgcgcug gacgccgccg uggucgccgc caccucccag     2160

agcaugcugc agcgccucac cacccuguuc cagaaggcgg agcgggaggg cgggcgccgg     2220

gacuucgugg gccuggagcc cccguggccc ggguuccgcg ccgaccucga ggccaagcug     2280

gagggcgugg ucgugucccg cgccgagcgg cgccgggccc gcggggcggg ccacaaggac     2340

acgauccggg ccaucgggga gggcgacgcc ggccccgugg ucuacgagcg caagccggug     2400

gccgcccuga ccgagaagga ccucgaccgg gugaaggacc ccgagcgcaa caagguccug     2460

aucgagaacc ugcggcagug gaucgcggcc gggaagccca aggaccccga caaggccccg     2520

cgcagcccca agggcgaccc caugaccaag gugaagcucg ugaccacgga cgacguccgg     2580

auccuggugc gcgggggcac cgccgaccgc ggggacaugg cccgggugga cgucuuccgg     2640

caggcggacc ccaagggccg cuggcgguuc uuccuggugc cgguguaccc ccaccagguc     2700

uucgacccca ccgacuggcc ccagccgccc aaccgcgccg ugguggccua caagcccgag     2760

aaggagugga ccgucauggg ccccgaccac gaguuccucu ggucccugua cccguucagc     2820

uggaucgccg uggagaaggc ggacgggacc cggaucgagg gcuacuuccg cgggaugcac     2880

cggagcacgg gcgccaucuc caucagcccc caccacucca agagccuggc cuccaccggc     2940

aucggggcca agacccucgc cagcuuccgc aaguucgcga uggaccgcau gggcaaccug     3000

uccgagaucg agcgggaggu gcgcaccugg cacggggucg ccugcacgug a              3051


<210>  1434
<211>  3054
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1434
augcgcgugg acagccuggg ccggugccuc uccgacgagg agugggcccg cgucaucuac       60

caccugugca agcaccgggg guuccacugg aucagccgcg ccgaggcgaa gcagaccgag      120

ggcgacucca agagcgaggg cggcaaggug aagcaggggc uggccggcac cgccaagcuc      180

auggacgaga agggcuaccg guccgccgcc gagauggugc uggcggaguu ccccgaggcc      240

cagcgcaaca agcaggggga guaccggaag gcccugagcc gcgagcuccu ggccaaggag      300

cuggcgcucc uguucgcccg gcagcgcguc uucggcaacc ggcacgccac ggacacccug      360

gagaccgcca uccucgggaa cuccgacaag aaguccggcc uguucuggca gcagaagccc      420

gcccuggcgg gggccgaccu ccugaagaug cuggggaccu gcacguucga gaagaacgag      480

uaccgcgccc ccaaggccag cuucaccgcc gagcggcacg uguggcucac ccgccugaac      540

aaccugcgga ucgugaccga cggcaucacg cgccagcuca ccgagcagga gcggcagcug      600

guccugcccc uccccuaccg ccaggcgggg gaccugaccu acaagcagcu gggcauggcc      660

cucaccaagg ccgggcugcu ggaggccggc ugcuucaagu ucaucggccu cgccuacccg      720

acggaggcgc agaaggccga ggggaagacc aaggaccccg agagcgccac ccuggugaag      780

auccccgccu ggcaggagcu gcggcagacc cucaacaagg ccucccugaa cgacgagugg      840

gagaagcuga gcggcgacgc gcucugcggg cagcccgaga ucuucgacaa gaucgccugg      900

auccuguccg uguacaagga cgacgacgag guccgcacgg agcuggccaa gcucgugcug      960

ccgaaccccg agggccugac cgaggcccuc cugaacaucc gguucgacaa guuccacgcg     1020

cugagccuca aggcccugcg cgccaucgug ccccacaugg agaaggggcu gcgcuacgac     1080

gaggccugcg uccaggccgg cuaccaccac ucccagcucc acaagaucgg cgagggggag     1140

cacaaguacc ugcccccgcu guacgacggc cgggacgggg acggccgcau gaucuucgcg     1200

gaggacgccg acaucccccg gaaccccgug gugcugcgca gccucaacca ggcccggaag     1260

gucgugaacg ccaucauccg ccgguacggg ucccccagcg ccgugcacau cgagauggcg     1320

cgcgaccugu cccggccguu cgacgagcgc aacaagauca agaaggacca ggacgaguac     1380

caggcccgga accagcgcga ccgggagcag uucaucgagc uguucggcca cccgcccaag     1440

ggccucgagu ucgagaagug gcgccuguac cgggagcaga acgggcagug cgccuacagc     1500

cagaagcccc uggccagcuc cggcgacguc gccgagaucu uccucgacgg gagcaccgag     1560

aucgaccacg cgcugcccua cucccgcagc uucgacgacu ccaagaacaa caaggugcug     1620

gugcucaccc gggagaaccg cgacaagggc aaccggacgc ccuacgagua ccucgggggc     1680

gccgagaaca gcgagcgcug gcagcgcuuc uucuccuucg ucgagagcaa caaggccuac     1740

cggcuggcca agcgcacccg gcugcugcgc aaggacuucg gcgcgaagga guccgaggag     1800

uuccgggagc gcaaccugaa cgacacccgg uacgugugcc gcuucuucaa gaacuacgug     1860

gagcagcacc ucaagcuggc cgaggggagc gaggccaagc ggugcgucgu ggucuccggc     1920

cagcugacca acuuccuccg cacgcggugg gggcugcuga agauccgcag cgacuccgac     1980

cggcaccacg cccucgacgc cgcgguggug gccgccugca gccacuccau gguccagcgc     2040

cugagcaacu acucccggac ccgcgagcug gagcaggucc gggacggcuu cgugaacauc     2100

gagaccggcg agaucgugaa ccccgccaug caccagcagc uccgcgagca cuuccccgac     2160

ccguggcccu acuuccggca cgagcuggag gcccgccuga agaucgacag ccccgcgcuc     2220

cugcgggagg agauggcccg ccuguccagc uacaccgagg aggagcucgc cgcccugaag     2280

ccccuguucg ugagccgcgc cccgcagcgg cgcaacgggg gcgcggccca caaggacacg     2340

aucuacgggc agcccgagaa gcucaagaag aacggcuccg ugagccagaa ggucgcccug     2400

gccucccugg cgcucaagga caucgaccug cuggccgacc cccaccggaa cgccaagcuc     2460

uacggggcca uccgcgagcg gcuggaggcc cacggcggca agggggacaa ggcguucccg     2520

gccaccaacc cccugcgcaa gcccgacaag gagggcaacc ccaccgggcc gaucgugcgg     2580

accgugacga aggucaucga caagcucagc ggcauccccg ugcgcggggg caucgccaag     2640

aacgacacca ugcugcgggu ggacgucuuc accaaggccg gccgcuucca ccuggugccc     2700

guguacgucc accaccgggu gaccgggcuc cccaaccagg ccaucacgcg cggcaaggac     2760

gagaaccagu gggcgaucau cgacgacacc uucgaguggc gguucucccu guacccgaac     2820

gaccuggugc agauccgccu caaggacgag acccaccugg gguacuacgc cagcaccgac     2880

cgguccacca gcuccaucag caucugggcc cacgaccgca accaguccgu cggcaaggac     2940

gggcugaucc ggagcaucgg cgugacgacc gccaaggaca ucgagaaguu ccacguggac     3000

acccucggca acgucuaccc cgccccgccc gagaagcgcc gggggcuggc guga           3054


<210>  1435
<211>  3066
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1435
augcgccggc gccgggaccg cuucaagcag cggcaggccg cccugcucaa gcaccuggcg       60

ggcguggggc uguuccccgc cgacgaggcc gcccgccggg agcucgccgg ccuggacccg      120

uacgagcugc gcgcgcgggc ccuccacgag gcccugcccc ugaaccacau cgggcgcgcc      180

cucuuccacc ugaaccagcg gcgcggcuuc aagagcaacc ggaagaccga ccgcgggaag      240

gcggacgacg agggcaagau ccggcugggc gucgcccgcc uccaggaggc caugcacgag      300

aagggguccg ccacccuggg cgaguuccug cacgggcggc gcgccgcggc cacggacccc      360

aaccagaucc ccagcgugcg gacccgccuc cgggccgagu ccggcgaggg ggcccgcggc      420

gacggcuacg acuucuaccc ggaccgccgg augcuggagg acgaguucga ggccaucugg      480

gagagccagc agguccacca ccccgcggug cugaccgacg agaucaagca gcgcuucuuc      540

gaggugaucu ucuaccagcg gccccucaag cagcccgaga ucgggaccug cacgcuggug      600

gccggcgagc gccggcugcc gaaggcccac ccccucuucc agaagcgccg gcugcuggag      660

gagcucaacg cccugcgcau cgucuggccc ggggugcagg ccgagcugcu cucccgggag      720

cagcgcgacc uggcguaccu gaagcucaag gacaagcggg ccgugcgcuu cgagagccug      780

cggaaggccc ugaagcucga ccccgacgcc cgcuucaaca aggaguccga gaaccggagc      840

gagcugaagg gcgacgaggu cgccgcggag cugggcgcca agucccgcuu cgggggccgg      900

ugggggcaca ccgccccggc cgaccagugg gagaucguga gccgcaucca ggcgcucgag      960

uccgacgacg acgaggccca guucugcgug uggcugagcg agcgguacgg ccuggacucc     1020

gagcaggccc gcgccgucgc cgcggcccgc cucccccagg gguucggccg guucggccug     1080

accgccacca cgcgccugau caaggcccuc caggacgagg ugaucgugua cagcgaggcc     1140

guccagaaga ccgggcuggg ccaccacucc gacuuccgga acggggaggu gcuggcggag     1200

cuccccuacu acggcaucgc ccuggagcgc cacaucaugc ccgggaccgg cgacccgggc     1260

gacagcgacg agaugcgggu ggggcgcguc accaacccca cggugcacau cggccugaac     1320

cagcuccggc gcguggucaa ccggcugauc aagcgcuacg ggccgccccg gcagaucgcc     1380

guggagcugg cccgcgagcu gaagcucgac gaccagcaca agcgggagau caacggccgc     1440

aaccggcaga accgcgaggc cgcggagcgg cgcucccaga agcuggugga caucgggauc     1500

gccgacaagg gcgccaaccg ggcccugcuc aagcuguggg aggagcugaa ccccgacaac     1560

gcgcucgacc gccggugcgu cuacaccggc cgccagauca gcaucgagau gcuguucucc     1620

ggggccgugg aggucgacca cauccugccc uucgccgcca cccucgacga cagcaacgcc     1680

aacaagcugc ugugccuccg cgaggcgaac cggaacaagc gccggcgcag ccccuucgag     1740

gccuucggcc agggggccga cugggaggag aucgccgccc gggcggcccg ccugccccgg     1800

gagaagcgcu ggcgguucga gccggacgcc auggcccgcu ucgacaccga ggagaaguuc     1860

cuggcccggc agcucgugga cacgcaguac cugucccgcc uggcgcggga guaccucggc     1920

gugcuguucc ccgacaaggg cgaggggagc ggccgcgucu gggugucccc cgggcggcug     1980

accgagaugc uccgccggag cuggggccug aaccaccucc ugcccgacca caaccucggg     2040

ggcggcgccg accagccgaa gaaccgccgg gaccaccgcc accacgccau cgacgccauc     2100

guggucgcgg ugaccgaccg ccaccuccug caggccaucg cccgggaggc cgggcagcgc     2160

ggccacgagg ccgcgaaccg gaugaccgcc gaccugcccg ugcccuggcc ggacuucgac     2220

cgcgacgucg gccaggugau ccgccaggug gucguguccc accgggccga ccacgggacg     2280

gugagcaagg ccggggccgc gaagggccag gacuccaccg ccggccggcu gcacaacgac     2340

accgccuacg ggcugaccgg ccagacggac gagaagggga acaccaucgu cgugcgccgg     2400

gugccccuga ccgcccugaa gaagcccacc agcaucgagg ccguccgcga cgacgagcuc     2460

cggaaggcgc uguccgugug gacgcgcggc uacgacggga aggccuucga ggcccggcug     2520

agccgcuucc ccgagcucgg cccgcugcag uuccggggca uccgccgggu gcgcgucgug     2580

gagccccuga ccgugauccc cauccgggac uccagcgggc gcgucuacaa gggcuacaag     2640

ggggacucca acuaccggua cgacgugugg gagcucaagg acggcaagug gguggccgag     2700

gucgugagca uguucgacgc ccaccagucc agcuggaccu cccccgugcg ccgcgagaac     2760

ccgaccgcgc ggaagguccu gagccugcag cagaacgaca ucguggccau cgagcccgag     2820

gggaaggagc gcgagcucau gcggguggcc cagcugcgcc agggcggcca gaucacgcug     2880

gccgcgugcc acgaggccgg ggaccucaag cggcgcgacg ccgaguccaa cgaggucgac     2940

cccuucaagu acuucagccc caccgccggc gggcugaagc gggugcgcgc ccgccaggug     3000

cgggucgacg agcugggccg ggugcucgac ccgggcagcc gcgaccggga gcgcggggcg     3060

gccuga                                                                3066


<210>  1436
<211>  3066
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1436
augaaguaca cccugggccu cgacgugggg aucgccagcg ucggcugggc cgugaucgac       60

aaggacaaca acaagaucau cgaccugggg gugcgcugcu ucgacaaggc ggaggagucc      120

aagaccggcg agagccuggc cacggcccgg cgcaucgccc gggggaugcg ccggcgcauc      180

ucccggcgca gccagcggcu ccgccugguc aagaagcugu ucgugcagua cgagaucauc      240

aaggacucca gcgaguucaa ccggaucuuc gacaccuccc gcgacggcug gaaggacccc      300

ugggagcucc gguacaacgc ccugagccgc auccugaagc cguacgagcu cgugcagguc      360

cugacccaca ucaccaagcg gcgcggcuuc aaguccaacc ggaaggagga ccugagcacg      420

accaaggagg ggguggugau caccuccauc aagaacaaca gcgagaugcu ccgcaccaag      480

aacuaccgga cgaucggcga gaugaucuuc auggagaccc ccgagaacuc caacaagcgc      540

aacaaggucg acgaguacau ccacaccauc gcgcgggagg accugcugaa cgagaucaag      600

uacaucuuca gcauccagcg caagcucggg ucccccuucg ugaccgagaa gcuggagcac      660

gacuuccuga acaucuggga guuccagcgc cccuucgcca gcggcgacuc cauccucagc      720

aaggugggga agugcacgcu gcugaaggag gagcuccggg ccccgaccuc cugcuacacc      780

agcgaguacu ucggccugcu gcagagcauc aacaaccucg uccuggugga ggacaacaac      840

acccugacgc ucaacaacga ccagcgcgcc aagaucaucg aguacgcgca cuucaagaac      900

gagaucaagu acuccgagau ccggaagcug cuggacaucg agcccgagau ccucuucaag      960

gcccacaacc ugacccacaa gaaccccagc ggcaacaacg aguccaagaa guucuacgag     1020

augaaguccu accacaagcu caagagcacc cugcccaccg acaucugggg gaagcugcac     1080

agcaacaagg agucccugga caaccucuuc uacugccuga cgguguacaa gaacgacaac     1140

gagaucaagg acuaccugca ggccaacaac cucgacuacc ugaucgagua caucgccaag     1200

cugccgaccu ucaacaaguu caagcaccuc agccuggucg ccaugaagcg caucaucccc     1260

uucauggaga agggcuacaa guacuccgac gcgugcaaca uggccgagcu ggacuucacc     1320

gggagcucca agcucgagaa gugcaacaag cugaccgugg agcccaucau cgagaacgug     1380

acgaaccccg ucgugauccg ggcccugacc caggcccgca aggugaucaa cgccaucauc     1440

cagaaguacg gccucccgua cauggucaac aucgagcugg cgcgggaggc cggcaugacc     1500

cgccaggacc gggacaaccu gaagaaggag cacgagaaca accgcaaggc ccgggagaag     1560

aucagcgacc ugauccgcca gaacgggcgg guggccuccg gccucgacau ccugaagugg     1620

cgccuguggg aggaccaggg cgggcggugc gccuacagcg ggaagcccau ccccgugugc     1680

gaccuccuga acgacucccu gacccagauc gaccacaucu accccuacag ccgcuccaug     1740

gacgacagcu acaugaacaa gguccucgug cugacggacg agaaccagaa caagcggucc     1800

uacaccccgu acgaggucug gggcagcacc gagaaguggg aggacuucga ggcgcgcauc     1860

uacagcaugc accuccccca guccaaggag aagcggcugc ugaaccgcaa cuucaucacc     1920

aaggaccugg acagcuucau cucccgcaac cucaacgaca cgcgguacau cagccgcuuc     1980

cugaagaacu acaucgaguc cuaccugcag uucagcaacg acucccccaa gagcugcgug     2040

gugugcguca acggccagug caccgcccag cuccgguccc gcugggggcu gaacaagaac     2100

cgggaggaga gcgaccugca ccacgcccuc gacgccgccg ugaucgcgug cgccgaccgc     2160

aagaucauca aggagaucac caacuacuac aacgagcggg agaaccacaa cuacaaggug     2220

aaguaccccc ugccguggca cuccuuccgc caggaccuga uggagacccu cgccggcguc     2280

uucaucagcc gggcgccccg ccggaagauc acggggcccg cccacgacga gaccauccgc     2340

ucccccaagc acuucaacaa gggccugacc agcgugaaga ucccgcugac caccgugacg     2400

cucgagaagc uggagaccau ggucaagaac accaagggcg ggaucuccga caaggccgug     2460

uacaacgugc ugaagaaccg gcucaucgag cacaacaaca agccccugaa ggccuucgcc     2520

gagaagaucu acaagccccu gaagaacggc accaacgggg ccaucauccg cagcauccgg     2580

gucgagacgc ccuccuacac cggcguguuc cgcaacgagg ggaagggcau cagcgacaac     2640

agccucaugg ugcgggucga cguguucaag aagaaggaca aguacuaccu ggugccgauc     2700

uacgucgcgc acaugaucaa gaaggagcug cccuccaagg ccaucgugcc ccucaagccg     2760

gagagccagu gggagcugau cgacuccacc cacgaguucc uguucagccu cuaccagaac     2820

gacuaccugg ugaucaagac caagaagggg aucacggagg gcuacuaccg cuccugccac     2880

cggggcaccg ggagccuguc ccucaugccc cacuucgcca acaacaagaa cgucaagauc     2940

gacaucggcg ugcgcaccgc caucagcauc gagaaguaca acguggacau ccuggggaac     3000

aaguccaucg ucaagggcga gccccggcgc ggcauggaga aguacaacag cuucaagucc     3060

aacuga                                                                3066


<210>  1437
<211>  3072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1437
augaagaucc ugggcuucga caucggggug gccagcaucg gcugggccuu cgucgagggg       60

gacgagaucc gcgacugcgg cgugcggauc uucaccaagg cggagaaccc caagaccggg      120

gagucccucg cccugccgcg ccgggaggcc cgcggcgugc ggcgccggcu ggcccgccgg      180

cgcguccggc ucaacacgau caagaagauc aucugcaagg aguucgaccu gcgccugagc      240

gacuacaucg ccgacgacgg cgagcucccc aaggcguacc agaccaacaa ggacaccaag      300

ucccccuacg agcugcggua caaggcccug aacgagcgcc ucagcaagga ggagcuggcc      360

cgggugaucc ugcacaucgc caagcaccgc ggguacggca acaagcacgc gaaggacggg      420

gacgacaagg acuccggcaa ggugaagaag gccaucgggg agaacaagac ccuccuggag      480

cagaagggcu acgagagcgu cggcgaguac ugcuuccggg aguucuucaa guccaucaag      540

gacaaggacg gggagacgca caacaucccc agccagaaca uccgcaacaa gaagggcaac      600

uacgaccggu gcguguccca gaagcagcug gaggacgagc ucaagcugau cuucgccaag      660

cagcgcaagc ugggguucaa caaggaguuc gaggacgcca uccucgaggu ggccuucuac      720

cagcggccgc ugaaggacuu cgacgacaag aucggcgacu gcaucuucuu cgacaacgag      780

aagcgcgcgc ccaaggagag caucuccgcc aucgaguucg ucgcccugac ccgcaucauc      840

aacacccuca aguaccugag caagaccacg ggcgaggugu acgagaagga gaucaucaac      900

aagaucuggc agaagauccu ggagaacggg aagaugaccu acgccgagcu ccggaagauc      960

cuggcccugg acuccagcgu guucuucaag gacaaggacc ucgacuacac ccugggcauc     1020

aaggaggcgg agaagaucaa guucaucgag uucaagaagc ugaucaaguu caagaaggcc     1080

cucggguccu ucgacggccu gagccgcgag auccuggaca acaucgccau gcagaucgcc     1140

aucaccaagg accggcagcg ccuccuggag aagcuggagg ucuucccccu caacaaggag     1200

cagaaggaga accuguccca gcugaacuuc agcaaguaca ucgaccucuc ccugaaggcc     1260

cugaaccaga uccuccccuu caugcgggag gggcgccggu acgacgaggc gaaggacgac     1320

gccaagcugc aggagaaggc caagaacacg aagaaggagg aguuccugcc gccccucaag     1380

gaguucgacc ugaaccugac caaccccgug guggcccgcg cccucgcgga guuccggaag     1440

guccugaacg cccugcugaa gaaguacggc aaggugcaca agauccaccu cgaguucgcc     1500

cgcgacaugc ggcuggacuu cgacgagcgc gagcggcuga ucagcaucca gaacgacaac     1560

uucaacaagc gccagcucgc cgaggacuuc ugcaaggagc acggccugga gcccagcaac     1620

gcgaacaucc ugaagaucaa gcucuggcag gagcagcagu ggcacugcau guacuccggg     1680

gagcugaucg gcaucccgga ccugaaggac cccaccaagc uccaggugga ccacaucuac     1740

cccuacagcc gguccuucga cgacagcuac accaacaagg uccuggugcg cgccgagugg     1800

aaccaggaga aggggaacca cacgcccuuc gaggccuucg gcggggacaa gaacaagugg     1860

gacaagaucg uggcccuggc cgagaagcuc ccgcccucca agaagcugaa ccggagcgag     1920

aaguccaaga aggacaagcu gcucgacgag aaguucaacg acaaggagcu gggcuuccug     1980

gcgcgcaaca ucgucgacac cggcuacauc gcccggcucg uggccaagua caucgacaug     2040

uaccuggagu uccugccccu cgaggagaac gagaacacca gccuggcccg cggggagaag     2100

ggcuccaaga agcacaucgg gaucgugaac ggccgccuga ccagcgaggu ccggcacaac     2160

ugggggcucg accccaagga ccgcgacaac caccuccacc acgccaucga cgcgaucgug     2220

aucgccuaca ugaacgacaa gcggaucaag gccuucgccg acuuccgccg gcugaaggag     2280

cugaagauca agcgcuacgg ccgggagauc acggaggacg aguaccagga gaagaagaag     2340

gagcuguccu gcgccccguu cgacgacuuc cgcaaccgga cccuggagaa caucgacaag     2400

aucuucguca gccgcccgcc ccggaagcgc gcgcggggca cccuccacga ggagaccuuc     2460

uacuccaucg acgacaagaa gcugcugaag gccuacgggg gcaaggaggg gaugcagcgc     2520

gcccucagcc ugggcaagau ccggcagauc ggcacgaaga ucgugaagaa cgggaccaug     2580

gugcgcgucg acaucuucaa ggacaagcgg ggccgcuucu acggggugcc caucuacacc     2640

auggacuucg cccugggcgu gcuccccaac aaggcggccg ucuccgggaa ggacaagaac     2700

ggccugauca aggagugggc cgagauggac agcggcuacg aguucugcuu cucccuguuc     2760

aaggacgacc ucauccuggu gcagcggaag gagaugcagg agcccgagcu gugcuacuuc     2820

gugagcuucg acuccagcaa cgcccagauc gacguccgca gccacaacaa ccacuucucc     2880

gugcucaacg agaaccagaa gcugcuguuc aagagcgcca cccaggcgga ggugaucggg     2940

gaguccaucg gcauccagaa ccucaagguc uucgagaagu ggcacgugag cccccugggg     3000

gaggugcgga aggccgaguu cagcccccgc ggcgaggcca agcugaagac guccaagaag     3060

cgcaagaagu ga                                                         3072


<210>  1438
<211>  3072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1438
augaagaucu acagcuucga caucggcgug gccuccaucg ggugggccuu caucgagaac       60

gagguccugc aggacugcgg cauccgcauc uucaccaagg cggagaaccc caagaacggg      120

gccagccucg cccugccgcg gcgcgaggcc cggggcgugc gcaagcggcu ggcccgccgg      180

cgcgggcggc ucaacaccau caagcagcug cugugcaagg aguucggccu ccagcuggag      240

gacuaccugu ccaacgacgg cgagcucccc aaggcguaca cggccagcaa ggccaacccc      300

cugaaguccc ccuacgagcu gcgcacccuc gcccugagcc agaagcugga cuccaaggac      360

cucgugcggg ucauccugca caucgcgaag caccgcgggu acggcaacaa gcacgccaag      420

gacagcaagg acaccgaguc cgggaaggug aagaaggcca ucgaguucaa ccggaagacc      480

cuggccgaga agggcuaccg cagcgugggg gaguaccucu accgggaguu cuuccagcag      540

ucccgcauca gccagaacaa cggcgccacg gaguucauca acguccggaa caaggcgggc      600

aacuacgagc acugcguggc ccaggacaug cugaaggagg agcuggaguc cauccucaac      660

gcccagcgcc aguucggguu cagccugaag caggacuucg aguccaagcu gcucaccaag      720

aucuucgagc agcggccgcu caagagcuuc gccgacaagg ugggcaacug cugcuucauc      780

gccggggaga agcgcgcgcc caaggacucc cccagcgcca ucgaguucau cgcccugucc      840

cggaccauca acacccugau caaccugagc aaggagagcg gcgagaucua caaccgcgcc      900

aucauccugc agaucgucga guccacgcuc aagaagggcg agaucagcua caaggagcug      960

cgcgagauca uccggcugga cgagcgccuc cgguucaagg acccccgccu ggacuacucc     1020

cgggagauca aggaggccga gaaggugaag uuccuggagu ucaagcagcu caagggguuc     1080

aagaaggcgc ugggcgagag cuucaccacc cuggccaucu cccagcucga cgagaucgcc     1140

gagcacaucg cccugaucaa ggaccgcgug aagcugaacg agaagcucga gagcuacgcc     1200

cugaccaagg agcagcggga ggcgcugucc aaccucagcu ucuccgccca caucaaccug     1260

agccugcagg cccucaagca gauccugccg uucaugcgcg gggacaacgg cgagcagugc     1320

cuggacuacg acgaguccgu cgagaaggcc gggcugaagg cgcucagcaa ggacacgcug     1380

aagggccaga ugcugccgcc ccucaacgag uucgagcccu accuguccaa ccccguggug     1440

gcccgggccc uggccgagua ccgcaagguc cucaacgugc ugcugaagca guacggcagc     1500

ccccacaaga uccacaucga guacgcccgg gaggcgaagc ucuccagcac cgagcgccag     1560

aaguacgaga aggagcagaa ggacaacuac gccgccaacc aggccgccca caagaagugc     1620

caggagcugg ggcuggagcc cuccagcacc aaccuccuga agcugaagcu cuggcaggag     1680

cagggcgggc ugugccugua cuccggcgcg aagaucacca ucagcuaccu ccaggacccc     1740

acggcccucc agguggacca caucuacccg uacagccggu ccuucgacga cagcuacaug     1800

aacaagugcc ugguccugac caaggccaac caggagaagg ggaaccgcac ccccuucgag     1860

gccuucggca ccgacuccgc caaguggggc gugaucacga cccuggcgca gaagcugccc     1920

cacaagaagc ggcgccggau ccucaacacc gccuucaacg acaaggaggc cggguucaag     1980

cugcgcaacc ugaacgacac cagcuacauc gcccggcucg uggcggacua cacggagacc     2040

uaccuggagu uccugccccu cgucgagucc gagaacacca cccugagcaa gggccagaag     2100

ggguccaaga agcacaucgc caucgugaac ggcgugcuga cgagcaccau gcgcuacuac     2160

cucggguucg ccuccaagga ccgcgacaac caccuccacc acgcccugga cgccgucauc     2220

aucggcuuca ugaacgacag cgugaucaag gcguucuccg acuucaagaa gacccaggag     2280

accagcaagg ccgccuacua cgcccacacg cuguccaagc aggaguacaa gaagcagcgg     2340

gccuucaucc agcugccgag cggcgagaac uuccgcgucu ccgugcugga gaagguggag     2400

agcgucuucg uguccaagcc gccccggaag cgcgugcgcg gggcgcucca cgaggccacc     2460

uucuacagcc ccaacgaccc caagcugcug aagaacuacg gcgggaccaa gggcgucgag     2520

cgggcccucg cccugggcaa gaugcggaag gugggggcca agaucgugag caacggcgcg     2580

augguccgcg uggacaucuu ccggcacaag uccagcggga aguucuacgg cgugcccguc     2640

uacaccaugg acuucgcccu ggggauccuc cccaacaagg ccgugguggu cggcaaggac     2700

cagaacggcg ugaucaagga cuggcuggag auggacucca gcuacgaguu caucuucucc     2760

cuguacaagg acgacgugau ccucguccag aagaaggaga ugcagcgccc cgagcugugc     2820

uacuucguga gcuucgacac guccggggcc agcaucaagg uggcgaagca cgacaacaac     2880

uucuccaccc ugaccgagaa ccagaagcuc cuguucagca acgccaccaa ggaggagguc     2940

gugggcaagu ccaucgggau ccagaaccug aagguguucg agaaguggca ggucagcgug     3000

cucggcgagg ugaagcggac ccaguccuac ccgcgcgaga acaucagccu ggccuccaag     3060

gccaagaagu ga                                                         3072


<210>  1439
<211>  3075
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1439
augauccgca cccugggcau cgacaucggg aucgccagca ucggcugggc cgugaucgag       60

ggggaguaca ccgacaaggg ccucgagaac aaggagaucg ucgcguccgg ggugcgggug      120

uucacgaagg ccgagaaccc caagaacaag gagagccugg cccugccgcg cacccucgcc      180

cgguccgccc gccggcgcaa cgcgcggaag aagggccgca uccagcaggu caagcacuac      240

cugagcaagg cccugggccu cgaccuggag ugcuucgugc agggggagaa gcuggccacc      300

cucuuccaga ccuccaagga cuuccugagc cccugggagc ugcgggagcg cgcccucuac      360

cgggugcugg acaaggagga gcuggcgcgc gucauccucc acaucgccaa gcggcgcggc      420

uacgacgaca ucacguacgg gguggaggac aacgacuccg gcaagaucaa gaaggccauc      480

gccgagaaca gcaagcggau caaggaggag cagugcaaga ccaucgggga gaugauguac      540

aagcuguacu uccagaaguc ccugaacgug cgcaacaaga aggagagcua caaccggugc      600

gucggccgcu ccgagcuccg ggaggagcug aagaccaucu uccagaucca gcaggagcuc      660

aagagccccu gggugaacga ggagcugauc uacaagcugc ugggcaaccc cgacgcccag      720

uccaagcagg agcgcgaggg gcucaucuuc uaccagcggc cgcugaaggg cuucggggac      780

aagaucggca agugcagcca caucaagaag ggcgagaacu cccccuaccg cgcgugcaag      840

cacgccccca gcgccgagga guucguggcc cugaccaagu ccaucaacuu ccucaagaac      900

cugacgaacc gccacgggcu gugcuucagc caggaggaca ugugcgucua ccucggcaag      960

auccugcagg aggcccagaa gaacgagaag gggcugaccu acuccaagcu caagcugcug     1020

cucgaccugc ccagcgacuu cgaguuccug ggccucgacu acagcgggaa gaacccggag     1080

aaggcggugu uccugucccu gcccagcacc uucaagcuca acaagaucac ccaggaccgg     1140

aagacgcagg acaagaucgc caacauccug ggcgccaaca aggacuggga ggccauccug     1200

aaggagcucg agucccugca gcugagcaag gagcagaucc agaccaucaa ggacgccaag     1260

cucaacuucu ccaagcacau caaccugagc cuggaggcgc uguaccaccu ccugccccug     1320

augcgcgagg gcaagcggua cgacgagggg guggagaucc uccaggagcg cggcaucuuc     1380

uccaagcccc agccgaagaa ccggcagcug cugccgcccc ucagcgagcu ggccaaggag     1440

gaguccuacu ucgacauccc caaccccguc cugcgccggg cccucagcga guuccgcaag     1500

guggugaacg cccugcugga gaaguacggg ggcuuccacu acuuccacau cgagcucacc     1560

cgggacgucu gcaaggccaa guccgcgcgc augcagcugg agaagaucaa caagaagaac     1620

aagagcgaga acgacgccgc cucccagcug cucgaggugc uggggcugcc caacaccuac     1680

aacaaccggc ucaagugcaa gcuguggaag cagcaggagg aguacugccu guacagcggc     1740

gagaagauca cgaucgacca ccucaaggac cagcgcgccc ugcagaucga ccacgcguuc     1800

ccccuguccc ggagccucga cgacucccag agcaacaagg ugcugugccu gaccuccagc     1860

aaccaggaga aguccaacaa gacccccuac gaguggcucg gcagcgacga gaagaagugg     1920

gacauguacg ucgggcgcgu guacagcucc aacuucagcc cguccaagaa gcggaagcug     1980

acccagaaga acuucaagga gcgcaacgag gaggacuucc uggcccggaa ccucguggac     2040

acgggcuaca ucgggcgcgu caccaaggag uacaucaagc acagccuguc cuuccugccc     2100

cuccccgacg gcaagaagga gcacauccgg aucaucagcg gguccaugac cagcaccaug     2160

cgcuccuucu ggggcgugca ggagaagaac cgcgaccacc accuccacca cgcccaggac     2220

gccaucauca ucgccugcau cgagcccagc augauccaga aguacacgac cuaccugaag     2280

gacaaggaga cccaccggcu gaagucccac cagaaggcgc agauccugcg cgagggcgac     2340

cacaagcuga gccuccggug gccgaugucc aacuucaagg acaagaucca ggagagcauc     2400

cagaacauca uccccuccca ccacgucagc cacaagguga ccggggagcu gcaccaggag     2460

acggugcgca ccaaggaguu cuacuaccag gccuucggcg gggaggaggg cgucaagaag     2520

gcccugaagu ucggcaagau ccgggagauc aaccagggga ucguggacaa cggcgccaug     2580

gugcgcgucg acaucuucaa gagcaaggac aaggggaagu ucuacgccgu gcccaucuac     2640

accuacgacu ucgcgaucgg caagcucccc aacaaggcca ucgugcaggg gaagaagaac     2700

ggcaucauca aggacuggcu ggagauggac gagaacuacg aguucugcuu cucccuguuc     2760

aagaacgacu gcaucaagau ccagaccaag gagaugcagg aggccguccu cgccaucuac     2820

aagagcacga acuccgccaa ggcgaccauc gagcuggagc accugagcaa guacgcccuc     2880

aagaacgagg acgaggagaa gauguucacc gacaccgaca aggagaagaa caagacgaug     2940

acccgggagu ccugcggcau ccaggggcug aagguguucc agaaggugaa gcugagcguc     3000

cucggcgagg ugcuggagca caagccgcgc aaccggcaga acaucgcccu gaagaccacc     3060

cccaagcacg uguga                                                      3075


<210>  1440
<211>  3078
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1440
augggcgggu acaugcccca gcugaugcgc uaccggcucg cccuggacau cggcagcacc       60

uccaucgggu gggccaugau ccgccugagc augcagaacg agccgaucgc gaucauccgg      120

augggcgugc gcaucuucuc cgacgggcgg aaccccaagg acggcagcuc ccucgccguc      180

acccgccggg uggcccgcca gaugcggcgc cggcgcgacc ggcugcugaa gcgcaagacg      240

cggcuccuga acgcccuggu gcagaagaag uucuuccccg aggacgccga cgcgcgcaag      300

gcccucgucg ugcugaaccc cuacgagcug cggaaggagg gccucuaccg ccagcugagc      360

gggccggagu ucggccgggc ccuguuccac aucucccagc gccggggguu ccucagcaac      420

cgcaagaccg acaagaagga caacgacucc agcgugcuga agaaggccau cgucgaccug      480

cgccagcagc ucgaggugga gaacugccag accaucggcg aguggcuguu caagcagcag      540

caguccggca agagcgugcg ggcgcgccug cgggggaaga cccagaagga caaggccuac      600

gacuucuaca ucgaccgcgc caugaucgcc cacgaguucg acacgcucug ggucucccag      660

gccaaguacg acccccugcu guucaacgag gacgcgcggg ccauccuccg cgacauccug      720

cuguaccagc ggccccucaa gcccgugaag ccgggccgcu gcacccugau ccccgacgag      780

agccgggcgc cccuggcccu cccccugacc cagcaggccc gcaucuacca ggagcugaac      840

caccuccgga ucaccacgcc ggcccugggg gcccaggccc ugaccguggc ccagcgcaac      900

accaucgccg cgcuccugga gaaguccgac gucaccuucg ugaagaucgc caaggcccug      960

ggccucacga gcaccaccga guucaaccug caggacauca agcgggaccg ccugaagggg     1020

aacgccaccg ccaucauccu cgcgcggaac caguacuucg gccgccggug gcacgacuuc     1080

aacgccgacc ugcagaaccu gaucguggac aagcucguca acgagggcuc cgagacggcc     1140

cugaucgagu ggcugaaggc caacaccgac guggacgaga gcgcggccga gcgcgugucc     1200

ggggucaccc uccccgaggg cuacgggagc cugagcgugg ccgcccugcg gcgccugcuc     1260

cccgcccuga aggaggacgu ggucguguac uccgaggcgg ugaagcgcgc cggcuucgac     1320

agccacuccg cccugagccu caucgagcag accggggagc uguaccagga gcugcccuac     1380

uacggcgagg uccuccagcg gcacgugggc uucggguccg gcgacccgaa cgacagcccc     1440

gagcgccggu ucgggaagau cgccaacccc acgguccaca ucggccugaa cgagcugcgc     1500

aagaucauca acgcccucau caagcgguac gggaaccccg cggagguggu ggucgagcug     1560

gcccgcgacc ugaagcucuc ccgggagaag cgcaaggaga uccaggagga gcagaagcag     1620

cggcaggacc gcaacgagaa gcggaucaag gacgccugcg agauccuggg caucgacccg     1680

cuggugaucg accgcggcaa gcggcgcgag aucagccaga agaugcagcu cugggacgag     1740

cugaacccca aggacgccgc caaccggcgc ugccccuaca ccggggacca gaucuccauc     1800

gagaagcugc ucagcgcgga gguggagauc gagcacaucc ugcccuucuc caagacccug     1860

gacgacagcc ucaacaacaa gaccgucgcc augcggcgcg ccaaccggga caagggcaac     1920

cgcacgccgu acgaggccuu cggggagucc cugaaccccg acuacgacua cgacgcgauc     1980

cugcagcggg ccagccucau gccccgcgag aagcgcaagc gguucgccuc cgacggcuac     2040

cagcaguggc ugcgcgacga caaggacuuc cugagccggg cccucaacga caccgccuac     2100

cugucccgca ucgcgaagga guaccugacc cucaucugcc cgcccaacag cgugcgggcc     2160

auccccgggc gcaugaccgc ccagcugcgg gccauguucg gccugaaccg ccuccugucc     2220

gagggcggga ucaagaaccg ggaggaccac cgccaccacg ccaucgacgc ggccgugauc     2280

ggcgucacgg accaggggcu gcuccagaag uucagccggg ccagcgcccg cacccgggag     2340

cugcagcugu cccgccucgu ggacgacaug ccccugccgu gggacaccua ccgggagcac     2400

gugacccgcg cccuggagca ccucgucgug agccacaagc ccgaccacgg cuaccagggc     2460

aagaugcacg aggacacggc gugggggcug cgggacaacg gccaggugac ccgccggguc     2520

cgcuccgagg acgggggccc ccgccagcgg cugcucgaga acaagaacgu ggugaagauc     2580

aacagcaccc gcgacuucuc ccggcacggg aucgacgagc agggccaggu caacgccuac     2640

aagggcuacg ucgggggcag caacuacugc aucgagaucu ggaucgacga gaaggggaag     2700

uggaacuccg acguggugag caccuuccag gccuaccaga ucgugcgcga gcugggcgag     2760

aaggccgcgu ggcagaagcu gcggaacccc cuccuguccc ugagcgccaa gccgcucgug     2820

augcgccuga ucaucgacga cgucgugaag augcagaucg ccgggcagaa caagaucaug     2880

cgggugguca agaucgccca gaacggccag aucuucuucg ccgagcacaa cgaggcgaac     2940

guggacgccc gcgacaagga caagaacaac cccuucaagu acaucuccaa gacggccggc     3000

agccugcaga aggccggggg cauccacaug gccaucuccc ccaucgggga cgcgcggccc     3060

gugcgcuacg gccgguga                                                   3078


<210>  1441
<211>  3084
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1441
augaaguaca cccugggccu cgacgugggg accaacagca ucggcugggc cguccuggag       60

cugaacacga acggggagcc ccagcgcauc gagaagcucg gcucccggau cuucaccgac      120

gggcgccacc cgaaggacgg caccacccug gccagcaagc ggcgccagaa gcggcacgag      180

cgccggcgcc gggaccgccu gcaccagcgg aagaagcucg ugcugaacca gcuggugucc      240

augggccucu ucccccagag cgagcaggag cagcaggacc ugaagucccu ggacguccuc      300

acgcugcgcg cgaacggggu gagcaagcag cuguccgccu acgagcucgg ccggguguuc      360

uacuaccuga accugaagcg cggguucaag agcaaccgga agggcggguc cgaggaggac      420

aaggccaaca agaucagcga gcgcaucaag aagcuccagg agcagcugaa ggagaacgac      480

uucaagaccg ucggccagua ccugcacgac cgguacauca agggccucuc caccaaggcc      540

accaucgaga acgacuucca ccuccugcgc cagcugaucg aggacgaguu cgacuucauc      600

guggagacgc agagcaaguu cuuccccgag aucuccgacg agcaguggaa cacccugcgg      660

aaccgcaugu uccaccagcg gccccugaag ccgguggaga ccgggaccug cagccucuuc      720

gaggacaaga agcgcacgua caaguacaug cccuccuucg aggaguaccg cuuccugacc      780

gagcuguuca accucagcua ccaggacgag aacuuccacu cccacagccu guccagcgag      840

cagaucuuca aggucuucca cgagcugaag aacacccggg aggccaccua cacgaagauc      900

aagaagcucc ugaagcugcc cagcgaccuc uccuucagca ucgagcgcuc caagagcaag      960

gacaagauca agaucuccac caccaaccac uucuucagca agucccugcu guucgcggag     1020

gagugggcca gccucgaccu gaagcugaag gacgaggugg ccgagaucua cuucuccgac     1080

augagcgugg aggagaucaa gaagggccuc cugcagcuga agaucgacuc cgagaucauc     1140

gagaaccucc uggaggagaa gguccccaac gugcaggagg cgaccugcag cuacuccaag     1200

gaggcccugc agaagaucgu gagccucauc cuggaggagg agcggcaccc gacgcugauc     1260

gucgacgacc uguucaagaa cgagaaggag gagaccuccc ucaucgaccg ccuggacuac     1320

uacggggcca gcauccccga cuccaugcag cccauccccc agcacaucaa ggacaacaac     1380

cugacccuca acgaggacga gaagcgguac ggcaagaucg ccaacccgac cgugcacgcc     1440

gcccugaacc agauccgcca gguggucaac gaccugaucg agcaguacgg caagcccacg     1500

aacauccaca ucgaguucgc gcgggaccuc aagaagucca aggaggagcg cgacuucgac     1560

aaccggaaga agaagaagaa cgaggagcug aacgagcagg uccgcgacuu caucgagaag     1620

cacaaccaga agcccagcgc cuucaacuuc gagcggguga agcugugguu cgagcucgac     1680

gccaugaaca accaggugug cgccuacagc gggaagacca ucagcgcccg cauggugcug     1740

uccgacgagg ugcaggucga ccacauccug cccuucagcc ggacccucga cgacucccug     1800

gcgaacaagg ugcugguccu cgccagcgag aacgugaaga agcgcaaccg gaccccguac     1860

gaggccuucg gccaggacac ggagaagugg gccuccaucc aggagcuggc caagcgccug     1920

cccuaccaca agcaguggcg guucgcggag gacgccauca agaaguucga ggagaaggac     1980

caguuccucc agcgccaccu gaacgacacc cgguacauca gcaagaucgc caagaaguac     2040

cuggggucca ucaccgaccc cuacaagauc guggccagcc gcggccagau gaccuccauc     2100

auccgcggga agcucggccu gaaccaguuc auccagaagc ccgacggcac gaagaaccgg     2160

gaggaccacc gccaccacgc caucgacgcg cugaccgucg cccucaccag ccgguccuac     2220

cugaagcgca ucagcgacgc cuccgcccgg aaccaggacc cgaaccgcau ccccaucccc     2280

gagcccugga agggguucau caaggacgcg gacgugaagu ucaacgagau caucgugagc     2340

cacaaggucg accacggcaa gaacgggccg uucauggagg agaccugcuu cggccugauc     2400

aagaacccca acaaguacga ggaggagaac gacuucaagc ucaucacgac caagucccug     2460

gacagcguga aggaccccuc caccauccgg agccagagcc ugcagaaccu cgccgagaag     2520

uccgggguga ucagccuggc caaguccggc gucaagaagc ugcgcgugua cgacgugacc     2580

aaggagaacc ccgccgacau cggccucauc gagagcgggc uggccaagau cacccacggc     2640

aaggacaagc agcacgucaa gcacuaccag aagggggaca ucaacuaccu ggcgaucugg     2700

ugccucccgc agaacaucac gcugaagucc gacaaccaca agaagcggaa gaccgacuac     2760

aucuucaccg ccaucaagac cuucgaccug aacagcaaga accccaacga ccuccgcccc     2820

cacccggccg ccaagcuggu gacgaaggug uacaagggcg acaccgucgc ccuggaggug     2880

aacaaccagc ucaacuacua caucgugaag uccauccggg cggccaacaa gaagcuguac     2940

uuccugaaga ucaaccagag cuccgagcuc gccaacgaga cccaguucau ccccgccuac     3000

accaagcugg ucgacuacaa guuccgcaag gugagcgugu cccccacggg ggucaucacc     3060

gacaacggcc cccugcucaa guga                                            3084


<210>  1442
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1442
augcgcagcc ugcgguaccg ccucgcccug gaccugggcu ccaccagccu cgggugggcc       60

cuguuccggc uggacgcgug caaccgcccc accgccguga ucaaggccgg cguccggauc      120

uucuccgacg ggcgcaaccc gaaggacggc agcucccucg ccgugacgcg gcgcgccgcg      180

cgggccaugc gccggcgccg ggaccgccug cugaagcgga agacccgcau gcaggccaag      240

cucguggagc acggguucuu ccccgccgac gcgggcaagc ggaaggcccu ggagcagcug      300

aaccccuacg cccuccgcgc caagggccug caggaggccc ugcuccccgg ggaguucgcg      360

cgggcccugu uccacaucaa ccagcgccgg ggcuucaaga gcaaccgcaa gaccgacaag      420

aaggacaacg acuccggggu ccugaagaag gccaucggcc agcuccgcca gcagauggcc      480

gagcagggga gccggaccgu gggcgaguac cuguggacgc gccugcagca gggccagggg      540

gugcgggccc gcuaccggga gaagccguac accaccgagg agggcaagaa gcgcaucgac      600

aaguccuacg accucuacau cgaccgggcg augaucgagc aggaguucga cgcccugugg      660

gccgcccagg ccgcguucaa ccccacccug uuccacgagg ccgcccgcgc cgaccucaag      720

gacacgcugc ugcaccagcg gccccuccgc cccgucaagc cggggcggug cacccugcug      780

cccgaggagg agcgcgcgcc ccucgcccug cccagcaccc agcgguuccg cauccaccag      840

gaggugaacc accuccggcu gcuggacgag aaccugcgcg agguggcccu cacccuggcc      900

cagcgggacg ccgucgugac ggcgcuggag accaaggcca agcucuccuu cgagcagauc      960

cgcaagcugc ugaagcucag cggcuccgug caguucaacc uggaggacgc caagcggacc     1020

gagcugaagg gcaacgccac cagcgccgcg cucgcccgca aggagcuguu cggggccgcc     1080

ugguccggcu ucgacgaggc ccugcaggac gagaucgucu ggcagcucgu gacggaggag     1140

ggggagggcg cgcugaucgc cuggcugcag acccacaccg ggguggacga ggcccgcgcc     1200

caggccaucg ucgacgugag ccugccggag ggcuacggca accucucccg gaaggcgcug     1260

gcccgcaucg ugcccgcccu gcgggccgcg gucaucaccu acgacaaggc cgugcaggcc     1320

gccggguucg accaccacag ccagcucggc uucgaguacg acgccuccga gguggaggac     1380

cugguccacc ccgagacggg ggagauccgc agcguguuca agcagcugcc cuacuacggc     1440

aaggcgcucc agcggcacgu ggccuucggg agcggcaagc cggaggaccc cgacgagaag     1500

cgcuacggca agaucgccaa ccccaccguc cacaucgggc ugaaccaggu gcggaugguc     1560

gugaacgccc ugauccgccg guacggccgc cccaccgagg uggucaucga gcucgcccgg     1620

gaccugaagc agucccgcga gcagaaggug gaggcgcagc ggcgccaggc cgacaaccag     1680

cggcgcaacg cccggauccg ccggagcauc gccgaggugc uggggaucgg cgaggagcgc     1740

guccgggggu ccgacaucca gaaguggauc ugcugggagg agcucagcuu cgacgccgcg     1800

gaccgccgcu gcccguacuc cggcgugcag aucagcgccg ccaugcugcu guccgacgag     1860

guggaggucg agcacauccu ccccuucagc aagacccugg acgacucccu gaacaaccgg     1920

acgguggcca ugcgccaggc gaaccggauc aagcgcaacc ggacccccug ggacgcccgc     1980

gccgaguucg aggcccaggg cuggagcuac gaggacaucc uccagcgggc cgagcgcaug     2040

ccccugcgga agcgcuaccg guucgcgccg gacggguacg agcgcuggcu gggcgacgac     2100

aaggacuucc ucgcccgggc ccugaacgac acccgcuacc ugucccgggu ggccgccgag     2160

uaccuccgcc uggucugccc cgggacccgg gugauccccg gccagcugac ggcgcuccug     2220

cgcggcaagu ucgggcugaa cgacgugcuc ggccuggacg gggagaagaa ccggaacgac     2280

caccgccacc acgccgucga cgccugcgug aucggcguga ccgaccaggg gcugaugcag     2340

cgcuucgcca ccgccagcgc gcaggcccgg ggcgacggcc ucacccgccu ggucgacggg     2400

augcccaugc cguggcccac guaccgggac cacguggagc gcgccgugcg gcacaucugg     2460

gucucccacc gccccgacca cggcuucgag ggggccauga uggaggagac cagcuacggc     2520

auccggaagg acggguccau caagcagcgc cggaaggccg acggcagcgc gggccgcgag     2580

aucuccaacc ugauccggau ccacgaggcc acccagccgc uccgccacgg ggugagcgcc     2640

gacggccagc cccuggccua caagggguac gugggcgggu ccaacuacug caucgagauc     2700

accgucaacg acaagggcaa gugggagggc gaggugauca gcacguuccg ggcguacggg     2760

gugguccgcg ccggcgggau gggccggcug cgcaaccccc acgaggggca gaacggccgg     2820

aagcucauca ugcgccuggu gaucggcgac agcgugcggc uggaggucga cggggccgag     2880

cgcaccaugc ggaucgugaa gaucuccggc agcaacgggc agaucuucau ggcccccauc     2940

cacgaggcca acguggacgc gcgcaacacc gacaagcagg acgccuucac cuacaccucc     3000

aaguacgccg gcagccucca gaaggccaag acgcgccggg ucaccaucuc cccgaucggc     3060

gaggugcgcg accccggguu caagggcuga                                      3090


<210>  1443
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1443
augaaguacc gccuggcccu cgaccugggc agcaccuccc uggggugggc caucgugcag       60

cucaacgcga aggacgagcc cugcgccauc guccgggccg gcgugcgcau cuucccggac      120

gggcggaacc ccaaggacgg cagcucccug gccgugaccc gccggaacgc ccgcgcgaug      180

cggcgccggc gcgaccggcu gcucaagcgc aaggcccgga ugcagcgccu gcugaucgag      240

cacgaccucu uccccgccga cgaggccgcg cggaaggccc uggagacgcu gaaccccuac      300

gagcuccgcg ccaaggggcu ggacgccgcc cugaccccgg cggaguucgc ccgggcccuc      360

uuccaccuga accagcgccg gggcuucaag agcaaccgcc ggaccgaccg caaggacaac      420

gacuccggcg cccugaaggc cgcgaucggg ggccuccgcg cccagcugga caccgccggg      480

gccgacggcc gggcccgcac ggucggggag cugcucuggc ggcgcaugag cgaccccaac      540

gugcugaccg ugcgggcgcg cuaccgggag cagcgcauca ccaaggacga cggcaagacc      600

cgggucgaca aguccuacga ccuguacgug gaccgcgcca ugguggaggc cgaguucgac      660

gcccucuggg ccaagcaggc ggagcugagc cccacgcagu ucggcgaggc cgcccgggcc      720

acccugaagg acugccuccu gcaccagcgc aaccugcggc ccguccgccc ggggcggugc      780

acccucgagc ccgacgagcc ccgcgcgccc cuggcccugc cgucccagca gcgguuccgc      840

auccuccagg aggugaacca ccuccggcug cugggcgggc gccugcugga ggagagccuc      900

uccguggagc agcgcgacaa gcuggucgcc gcccuggagg acggcagcaa guccuucaag      960

accgccaucc ccaagcuccu gggccugccc gggacggugc aguucaaccu cgcggacgcc     1020

aagcggaccg agcugaaggg caacgccacc agcaugcugc ucgggaagaa ggacuucuuc     1080

ggccccgccu ggcacggguu cuccgaggcc cgccaggacg agaucgugau gcggauccag     1140

gaggaggaga gcgaggcgcg ccuggucgac uggcuggcca ccuccacggg cguggacgag     1200

gcccacgccc ugaagaucgc caacgcggcc cucccggagg gcuacgggag ccuguccgcc     1260

aaggcccugg cgaagauccu ccccgccaug cgggccgagg ugaucaccua cgacaaggcc     1320

guccuggccg cgggcuacga ccaccacagc cacaucagcc acgccgccuc caccggggag     1380

auccugcccg agcuccccua cuacggcgag uaccugcagc gccacguggg guucggcacc     1440

ggcgagcuga acgacacgcc ggagaagcgg uucgggcgca ucgccaaccc caccgugcac     1500

aucggccuca accagguccg gguggugguc aacgcccuga ucaagcgcua cgggcacccc     1560

accgagguga ucgucgaggu ggcgcgggac cugaagcaga gccaggacca gcgccgggag     1620

acccaggagc agcaggccaa gaaccagaag cgcaacgagc ggcuccgcaa ggacgccgcc     1680

gccauccugg gcaucgcgga cgagcgggug cgccuggccg acauccagaa gcucauccug     1740

ugggaggagc uguccuucga caucgccgac cggcgcugcc ccuacagcgg gguccagcuc     1800

uccgccgcga uggugcugag cgagcagguc gagaucgagc acauccugcc guucuccaug     1860

acgcucgacg acagccugaa caacaagacc guggccaugc ggcuggugaa ccgccucaag     1920

ggcaaccgca cccccuggga cgcccgggag gccuucgccg gccaggggug ggacuacgac     1980

ggcauccuga ugcgcgcgga gcggaugccc cgcgccaagc ggcagcgcuu cgcccccgac     2040

gggcuggaga aguggcucaa ggaggacaag gacuuccugg cccgggcccu gaacgacacc     2100

cgccaccucu cccggguggc gcgcgaguac cugagccugg ucugcccggc cuccacgcgg     2160

gugauccccg gccagaugac cgccaugcuc cgcgccaagu ucggccugaa cgacauccug     2220

gggcucaacg gcgagaagaa ccggaacgac caccgccacc acgccgugga cgcgugcguc     2280

guggccguga ccgaccaggg gcugcugcag cgguucgccc aggccagcgc cgacgcgcgc     2340

ggcaaggggc ucgaccggcu ggucgagacc augcccgacc cgugggacuc cuaccgcgag     2400

cacgugcagc gggccgugaa cagcaucugg gucucccaca agcccgacca cggcuucgag     2460

ggcgccaugc acgaggacac gaccuaccgc ccgccccacc uggaccccaa gaacaugugg     2520

cgcacccggg ggaucggcgg gggcaagccc aacgagaagg aggccgacag caaggcgaug     2580

gugggguuca ccggccccga gcaccuccag cgccaccacc gggacaaggc cggccagcag     2640

cugcccuaca agggguacgu ggccuccccg aaguucugca uggacaucgu ccgcaacgac     2700

aagggccggu gggaggggga gguggugagc acguucgagg ccuaccgccu ggucuccgag     2760

uucggccggg acgccgcgcu cgcccacauc cagagcaaga gccgcuccau cagcgggaag     2820

ccccugguga ugcggcugca gggcggcgac auggugcgcu ucgacgacga cgaggggcgg     2880

cccgucgugc uccgcguggu caacaugacc ggcaacgggc agaucuucuu cgcgccccac     2940

aacgaggcca acggcgacgc ccggaaccgc gacaagcagg accaguucgu guaccugucc     3000

aaguacgccg ggagccugca gaaggcccgg ggccgccggg ugaccgucuc cccggccggc     3060

gagcuccgcg accccggguu caaggaguga                                      3090


<210>  1444
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1444
augcgcagcc ugcgguaccg ccucgcccug gaccugggcu ccaccagccu cgggugggcc       60

cuguuccggc uggacgcgug caaccgcccc accgccguga ucaaggccgg cguccggauc      120

uucuccgacg ggcgcaaccc gaaggacggc agcucccucg ccgugacgcg gcgcgccgcg      180

cgggccaugc gccggcgccg ggaccgccug cugaagcgga agacccgcau gcaggccaag      240

cucguggagc acggguucuu ccccgccgac gcgggcaagc ggaaggcccu ggagcagcug      300

aaccccuacg cccuccgcgc caagggccug caggaggccc ugcuccccgg ggaguucgcg      360

cgggcccugu uccacaucaa ccagcgccgg ggcuucaaga gcaaccgcaa gaccgacaag      420

aaggacaacg acuccggggc ccugaagaag gccaucggcc agcuccgcca gcagauggcc      480

gagcagggga gccggaccgu cggcgaguac cuguggacgc gccugcagca gggccagggg      540

gugcgggcgc gcuaccggga gaagccguac accaccgagg agggcaagaa gcgcaucgac      600

aaguccuacg accucuacau cgaccgggcc augaucgagc aggaguucga cgcccugugg      660

gccgcccagg cggccuucaa ccccacccug uuccacgagg ccgcccgcgc cgaccucaag      720

gacacgcugc ugcaccagcg gccccuccgc cccgugaagc cggggcggug cacccugcug      780

cccgaggagg agcgcgcgcc ccucgcccug cccagcaccc agcgguuccg cauccaccag      840

gaggucaacc accuccggcu gcuggacgag aaccugcgcg agguggcccu cacccuggcc      900

cagcgggacg cgguggucac ggcccuggag accaaggcca agcucuccuu cgagcagauc      960

cgcaagcugc ugaagcucag cggcuccgug caguucaacc uggaggacgc caagcggacc     1020

gagcugaagg gcaacgccac cagcgcggcc cucgcccgca aggagcuguu cggggccgcc     1080

ugguccggcu ucgacgaggc gcugcaggac gagaucgugu ggcagcucgu cacggaggag     1140

ggggagggcg cccugaucgc cuggcugcag acccacaccg ggguggacga ggcccgcgcc     1200

caggcgaucg uggacgucag ccugccggag ggcuacggca accucucccg gaaggcccug     1260

gcccgcaucg ugcccgcccu gcgggcggcc gugaucaccu acgacaaggc cguccaggcc     1320

gccggguucg accaccacag ccagcucggc uucgaguacg acgcguccga gguggaggac     1380

cuggugcacc ccgagacggg ggagauccgc agcgucuuca agcagcugcc cuacuacggc     1440

aaggcccucc agcggcacgu ggccuucggg agcggcaagc cggaggaccc cgacgagaag     1500

cgcuacggca agaucgccaa ccccaccgug cacaucgggc ugaaccaggu ccggauggug     1560

gucaacgccc ugauccgccg guacggccgc cccaccgagg uggugaucga gcucgcgcgg     1620

gaccugaagc agucccgcga gcagaagguc gaggcccagc ggcgccaggc cgacaaccag     1680

cggcgcaacg cccggauccg ccggagcauc gccgaggugc uggggaucgg cgaggagcgc     1740

gugcgggggu ccgacaucca gaaguggauc ugcugggagg agcucagcuu cgacgcggcc     1800

gaccgccgcu gcccguacuc cggcguccag aucagcgccg ccaugcugcu guccgacgag     1860

guggaggugg agcacauccu ccccuucagc aagacccugg acgacucccu gaacaaccgg     1920

acggucgcga ugcgccaggc caaccggauc aagcgcaacc ggacccccug ggacgcccgc     1980

gccgaguucg aggcccaggg cuggagcuac gaggacaucc uccagcgggc ggagcgcaug     2040

ccccugcgga agcgcuaccg guucgccccg gacggguacg agcgcuggcu gggcgacgac     2100

aaggacuucc ucgcccgggc ccugaacgac acccgcuacc ugucccgggu ggccgcggag     2160

uaccuccgcc uggugugccc cgggacccgg gucauccccg gccagcugac ggcccuccug     2220

cgcggcaagu ucgggcugaa cgacgugcuc ggccuggacg gggagaagaa ccggaacgac     2280

caccgccacc acgccgugga cgccugcguc aucggcguga ccgaccaggg gcugaugcag     2340

cgcuucgcca ccgcgagcgc ccaggcccgg ggcgacggcc ucacccgccu gguggacggg     2400

augcccaugc cguggcccac guaccgggac cacgucgagc gcgccgugcg gcacaucugg     2460

gugucccacc gccccgacca cggcuucgag ggggccauga uggaggagac cagcuacggc     2520

auccggaagg acggguccau caagcagcgc cggaaggcgg acggcagcgc cggccgcgag     2580

aucuccaacc ugauccggau ccacgaggcc acccagccgc uccgccacgg ggucagcgcc     2640

gacggccagc cccuggcgua caagggguac gugggcgggu ccaacuacug caucgagauc     2700

accgugaacg acaagggcaa gugggagggc gaggucauca gcacguuccg ggccuacggg     2760

aucgugcgcg ccggcgggau gggccggcug cgcaaccccc acgaggggca gaacggccgg     2820

aagcucauca ugcgccuggu gaucggcgac agcguccggc uggaggugga cggggccgag     2880

cgcaccaugc ggaucgugaa gaucuccggc agcaacgggc agaucuucau ggcccccauc     2940

cacgaggcga acgucgacgc ccgcaacacc gacaagcagg acgccuucac cuacaccucc     3000

aaguacgccg gcagccucca gaaggccaag acgcgccggg ugaccaucuc cccgaucggc     3060

gaggugcgcg accccggguu caagggcuga                                      3090


<210>  1445
<211>  3096
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1445
augcccgcca ucuaccgccu ggcccucgac cugggcagca ccucccuggg gugggcggug       60

cuccggcugg agccggccgc ccuggagccc aucgccauca ucaaggccgg cguccgcauc      120

uucagcgacg ggcggaaccc caaggacggc gcgucccucg ccgugacccg ccgggccgcc      180

cgcgcgaugc ggcgccggcg cgaccggcug cugaagcgca aggcccggau gcaggacaag      240

cucgugaagc acggguucuu ccccagcgac auggacgagc gcaaggcccu ggagcggcug      300

gacccguacg cccuccgcgc caagggccug cgggaggcgc ugacgcccgg cgaguucgcc      360

cgcgcccucu uccacaucaa ccagcggcgc ggguucaagu ccaaccggaa gaccgacaag      420

aaggacaacg acagcggcgc ccugaagaag gccaucuccc accuccgcca gcagauggag      480

gccgggggcc accgcaccgu cggggaccac cuguggagcc ggcugcagca gggccugggc      540

gugcgcgccc gguaccgcga gacccccuuc acgaacgacg aggggaagaa gcggaucgac      600

aaguccuacg accucuacau cgaccgcgcc augaucgagc aggaguucga cgcccugugg      660

gccgcgcagg ccgccuucaa ccccgcccug uuccacgagg uggcccgggc ggaccucaag      720

gacacccugc ugcaccagcg ccggcuccgc ccggucaagc ccggccggug cacccugcug      780

cccgaggagg agcgcgcgcc ccucgcccug ccgagcaccc agcgguuccg caucuaccag      840

gaggugaacc accuccggcu gcugggggag gaccugcgcg agguggcccu cacgcuggcg      900

cagcgggacg ccgucgccaa cgcccuggag uccaaggccc gccucaccuu cgagcagauc      960

cggaagcugc ugaagcucag cggcuccgug caguucaacc uggaggacgc gaagcgcacc     1020

gagcugaagg gcaacgccac cagcgccgcc cucgcccgca aggagcuguu cggggcggcc     1080

uggggcgggu ucggcgaggc ccugcaggac gagaucgugu ggcagcucgu cacggaggag     1140

ggggagggcg cccugaucgc cuggcugcag gcgcacaccg gcguggacga ggcccgggcc     1200

caggccaucg cggacgccuc ccugcccgag ggguacggca gccucucccg caaggcccug     1260

gcccggaucg ugcccgcccu gcgcgcgggg gucgugaccu acgacaaggc cgugcaggcc     1320

gccggcuucg accaccacag ccagcucggg uucgaguacg acgccggcga ggucgaggac     1380

ugggugcacc ccgacaccgg cgagauccgg ccgguguuca agcagcugcc cuacuacggg     1440

aagccccugc agcgccacgu cgcguucggc uccgggaagc ccgaggaccc ggacgagcgg     1500

cgcuacggca agaucgccaa ccccacggug cacaucgggc ucaaccaggu gcggaccguc     1560

gugaacgccc ugauccgccg guacggccgc cccaccgagg ucgugaucga gcuggcccgg     1620

gaccucaagc agagccgcga gcagaaggug gaggcccagc ggaagcaggc ggagaaccag     1680

cgccggaacg cccgcauccg caagagcauc gccgaggucc ugggcaucgg ggaggagcag     1740

guccggggcg ccgacaucca gaaguggauc ugcugggagg agcuguccuu cgacguggcg     1800

gagcggcgcu gccccuacag cggggugcag aucuccgccc gcaugcuccu gagcgacgag     1860

guggaggugg agcacauccu gccguucucc aagacccucg acgacagccu gaacaaccgg     1920

acggucgcca ugcgccaggc caaccggauc aagcgcaacc ggacccccug gaccgcccgc     1980

gacgacuucg aggcgcaggg cugguccuac gagggcaucc ugcagcgggc cgagcgcaug     2040

ccccuccgga agcgcuaccg guucgccccc gacggguacg agcgcuggcu gggcgacgac     2100

aaggacuucc uggcccgggc ccucaacgac acccgcuacc ugagccgggu ggcggccgag     2160

uaccugcgcc ucgugugccc gggguccggc acgcggguca uccccgggca gaugaccgcc     2220

cgccugcggg gcaaguucgg ccugaacggg gugcucggcc ccgacgggga gaagaaccgc     2280

aacgaccacc gccaccacgc cguggacgcc ugcgucaucg gcgugaccga ccaggggcug     2340

augcagcggu ucgcgaacgc cagcgcccag gcccgcgagg acggccugac ccggcucgug     2400

gacgacaugc cgcgccccug gcccacguac cgggcccacg ucgagcgcgc ggugcggcac     2460

aucugggugu cccaccgccc cgaccacggc uucgaggggg ccaugaugga ggagaccagc     2520

uacggcaucg ggaaggacgg cuccaucaag cagcggcgca aggccgacgg gagcccgggc     2580

cgggagauca ccaaccugau ccgcauccac gaggccaccc agccccagcg gcacggcgcg     2640

gggcccgacg gccagccccu gccguacaag ggguacgucg gcggguccaa cuacugcauc     2700

gagaucacgc gcaacgacaa gggcaagugg gagggcgagg ugaucagcac cuuccgggcc     2760

uacgggaucg ugcgcaccuc cggccucggg cagcugcggc acccccgcgu cggccagaac     2820

ggccgggagc uggugaugcg ccucgugauc ggggacagcg uccggcugga gaucgacggc     2880

aucgagcgca ccaugcgcau cgugaagauc uccgggagca acggccagau cuucauggcc     2940

cccauccacg aggccaacgu ggacgcccgg aacagcgaca agcaggacgc guucgccuac     3000

acguccaagu acgccgggag ccugcagaag accaaggccc gcuucgucac caucuccccc     3060

aucggcgagg ugcgggaccu cggcuucaag ggguga                               3096


<210>  1446
<211>  3099
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1446
augaagaagc gcauccuggg ccucgacauc gggaucgcca gcgugggcug ggcccugguc       60

gaguucgacg acgaguucca ggacaacaag aagaagggga ccaucaucaa guccggcgug      120

cggaucuuca cccgcgcgga gacgcccaag gagcgggaga gccuggccaa gccgcgccgg      180

gaggcccgcu cccagcggcg ccggauccgc cggcgcgugg agcggcucaa cagcauccgc      240

gagcuguucg ucaagaacgg ggugguggag gagugguccg ucagcaagaa gaaccccaac      300

aacaucuaca ucacccugaa ccagaucaag acccccuggc agcuccggau ggagucccug      360

gaccgcaagc ugaccaacga gcaguucgcc guggugcuca cgcaccuggc caagcaccgg      420

ggcuacaaga gccugcgcaa ggacgacaag gcgggcaagg uccucgaggc caucucccag      480

aacaagaagc ggcugaacga cagcgacuac cgcacccugg gggagauguu cugcaagaac      540

gaguccuaca aggagcacaa gcggaacaag ggcggggagu acaucaacag cguggcccgc      600

gagcucaucg aggaggaggu ggagaagauc uucgucaagc agcgcgagaa gggcaacaac      660

uucgccgacg ugaacaccga gcgggaguac cugaagaucg cguucuccca gaagcccgcc      720

gggagcaucu ccaagauggu gggcuucugc accuucgaga acggcgagaa gcgcgccccg      780

cgggacagcu acuccgccga gcuguucaag guccucugca cgaucaacaa caccaccuuc      840

accgacauga agacggggga gauccgcagc uucuccaagg aggagaucgg caagacccug      900

gagaccguga agaacgugaa ggacgucaag uacgccaaga uccggaagga ccuggggcuc      960

gaggacagcg ugaaguucag cggcguggac uaccgcgaga acgcgaaguc caagggcgag     1020

ggggagaucu ucgucagcau gaagaccuac cacgagauca aguccaagau cgagaagcug     1080

agcaaggaga agugggacaa ccugaagggc aacacggagc uccuggacuc caucgugaac     1140

aucaucgcca accugaagga cgacgagagc aucaagaagg aguuccagaa gcucaagauc     1200

gacgaggaca ucaucgacgu gcugcugaac uucgaguuca agaaguucau caaccucucc     1260

uucaaggccc ugcggaagau cauccccuuc caguuccagg ggcagaagua caucaccgcc     1320

agcaccuccg ccggcuacag ccugaagcag gaggggauca agaacaagcu cgucccgccc     1380

gcgaaggaga acaucaacgu ggccgucgug gcccgcgccu ucgcccagac ccggaaggug     1440

aucaacgcgg ucauccgcaa guacggccag uucgaccaga ucaacaucga gcuggccacg     1500

gagcugaaga acuccaaguu cgaccggagc aagaucgagg agggccagaa gaaguuccag     1560

ucccagaagg acaagaucuu caaggaggug caggagcucc gcgggggcau gacccccagc     1620

uccagccugc ugcucaagua ccggcugugg cgcgagcagg acgagcggug cguguacucc     1680

gggcgcaaga ucagcuucga ggaccugcuc gacaccggcu uccuggacau cgaccacauc     1740

auccccuacu ccaagagcau ggacgacagc uucaacaaca agguccugug ccucgccgag     1800

gagaaccgga acaagaagaa cgacaucccc uucaacuacu accagcgcau cgaccgggac     1860

uggaaccugc ugaucuccau caucaccagc augaagaaca ugaagaucgc caagaagucc     1920

cgccuccuga agcaggagcu gagcgacauc gagggguuca ucgagcgcaa caucaacgac     1980

acgcgguacg ccacccgcua caucaaggac uuccuggaga acaaccucga guucaaggag     2040

aacgaguuca ucaaggugaa ggugcaggcg cgguccggcg gccugaccag cguccugucc     2100

uacaacuggg ggaugagcaa ggaccgcgag gaguccuacu uccaccacgc caaggacgcc     2160

aucaucgugg ccugcagcac ccagggcaug gugcaguaca ucuccaagau cagccagcag     2220

uacgagaacg acaaggacaa gaugaagaag aucaaggaca agacggagaa cugcaucccc     2280

aagccguggg acuccuuccg ggcggacguc gaggagagca ucgaggggau cuucgugucc     2340

uucgcgcccc gccacaaggu gaccggcagc gcccacaagg agaccaucua cuccaagaag     2400

caccucgaga aggagcggua cgucaccgug aagaaguccc uggacaagau caagcugagc     2460

gaccucgaga acauccccug caacgagaac ugcgggauca uccgcgugcu gaaggagcgg     2520

cuggagaagu ucaagaacga cagccucaag gccuucgccg accccgucca caugccgacg     2580

aagugcccca acaagaaggg ccccgugauc cgcagcguga agaucaagga guccaacaag     2640

accggcaucg agguccggaa ggggcuggcc gagcgcggcg agauggugcg gguggacguc     2700

uucaucaagg acaagaagua cuaccuggug cccaucuacg ugagcgacuu caagaacaag     2760

gaccucccga acaaggccau cgccgcccug aagcuggaga acgaguggau caugaucgac     2820

aagaccuacu ccuucaaguu cagccucuuc aaggacgacc ugaucaagau caagaagaag     2880

aaggaggaga ucuucgggua cuucggcggc guccaccgcu ccaccgggac gaucaagauc     2940

aagagccggc acaagcccaa ggagaucgac gagggcaucg ggucccgcug ccugcucgac     3000

uuccggaagu uccaggugga cgugcugggc aacuacaccg aggucaagca cgagaagcgc     3060

augcccgccu acaucacccg gaaggacaag aagcacuga                            3099


<210>  1447
<211>  3105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1447
augaagaucc ugggcuucga caucgggauc gccagcaucg gcugggccuu cguggagaac       60

ggggagcucc gcgacugcgg cguccggauc uucaccaagg cggagaaccc caagaccggg      120

gacucccugg ccaugccgcg ccgggaggcc cgcagcgugc ggcgccggcu ggcccgccgg      180

aagggccgcc ucgagacgcu gaagcggcug cucgccaagg agugggaccu gugcuacgag      240

gacuacaucg cggccgacgg cgagcugccc aaggccuuca uggggaagaa ccucaccaac      300

cccuacgugc ugcgcuacga ggcccugcag cggcuccugg gcaaggagga gcuggcgcgc      360

gucauccucc acaucgccaa gcaccggggg uacggcaaca agaacgccaa gaucaccaag      420

uccgaggaga gcaagcgcga gcaggggaag auccuguccg cccuggccac caacgcgagc      480

gugaucgccc gguaccgcac ggugggcgag uacuucuaca aggaguucug cgaggucauc      540

aagaaccccc agggccucaa caccaacgag aacugcaccc agccgaaggu gcgggugcug      600

aagcccaucc gcaacaaggg cggggaguac accaacugca uccugcagga ggaccuccag      660

cgggagcugc gcugcaucuu cgagcaccag aagggguucg gcuuccccau cacgcaggag      720

uuccaggaca agauccugaa gaucgccuuc uaccagcgcu cccucaagga cuucagccac      780

cuggucggca agugcaccuu cuaccccgac gagccgcggg cccccaaguu cucccugagc      840

gccaucgagu ucaucaccaa ggcgaagacc aucaaccucc ugguggggau cgugaaggag      900

uccggcgagg ucugggacaa ggagcagugg cgcgagcggc uggacagcgu guucuccgcc      960

gugugcgagc gcgggaaccu cacguucgcc cagaugcgga agauccugaa gaucgacgag     1020

cgcauccagu ucaaggaggu cgacuacacc gccaagaacc ccgagaacaa gaaguucgug     1080

gaguucaaga accugaagaa guucugcgag auccucggcg ccaucguggg ggaccgggcg     1140

cuggccgaca gcaucgcccg cgacaugacc cugaucaagg acgaggagga gcucgcccag     1200

aagcugcggg gcuacggcaa guucucccag gagcagauca cccagcuggg ggcccucaac     1260

uucagccacc acaucaaccu gucccugaag gcgcucagcg agauccugcc cuucaugcgc     1320

gagggcaugc gguacgacga ggccugccag gaggccaagc ugcaggccaa gcacaacgac     1380

aagaagucca aguuccuccc gcccuucugc gggagcaucu acgcggacga gcugacgaac     1440

cccgucgugc accgcgccau cgccgaguac cggaagaucc ugaacgcccu caucgccaag     1500

uacggcaacg ugcacaagau ccacaucgag cugacccgcg acgucgggaa gaacuuccag     1560

gagcgggaga aguacaagaa ggagaucgag agcaacuaca aggcgcgcgu gcaggccaug     1620

caggagugcg agaagcuggg ccugacccuc uccgagggca acauccugaa gcugcgccuc     1680

uucauggagc agaacgagcg gugcguguac agcgggcgga agaucacccu ggccaaccug     1740

aaggagcagg gcgcccucga gaucgaccac auccugcccu acucccgcag cuccgacgac     1800

agcuacauga acaagguccu gguguucacg aacgagaacc agaacaaggg gaacaagacc     1860

ccguacgagg ccuucggcgg ggacucccag aaguggggcg agaucgagag ccucgcgcug     1920

cgcuccggcu accccaagaa gaaggccaag cggauccugg acaagggguu cggcgaccgc     1980

gaggccgggu ucaagagccg gaacaucguc gacuccggcu acaucgcccg ccucaucgcc     2040

aacuacacca aggaguaccu gaaguuccug ccccucgaca gccacgagaa caccgcgcug     2100

aucgccggcg agaaggacuc caagauccac guggaggccg ugaaggggau gcugacggcc     2160

accaugcggc acuucugggg ccucgggagc aagaaccgcu acgagcacac ccaccacgcg     2220

aucgacgcca ucaucaucgc cuacaucaac gccgccauga uccagcgguu cucccaguuc     2280

cgccagaacc aggagagccu gaaggcgcgg uucuacgcca aggagcuggc caaggaggag     2340

uucaagaccc agcgcgccuu cuucgagccc uucuccggcu uccgggagca gguccucgcc     2400

aagguggggc agaucuucgu gagccgcccg ccccggaagc gcgcgcgggg cgcccugcac     2460

gaggagacgu ucuacuccau cgacgacaag aagcugagcg agaccuacgg cgggaaggag     2520

ggcguccagc gcgcccucag ccuggggaag auccggcaga ucggcaccaa gaucguggcc     2580

aacgggccca uggugcgcgu cgacaucuuc aagcacaaga aguccggccg guucuacgcc     2640

gugcccaucu acaccaugga cuucgcgcug ggcauccucc cgaacaaggc cguggucagc     2700

gggaaggaca acgaccgcau caucaaggac uggcuggaga uggacuccaa cuacgaguuc     2760

ugcuucagcc uguucaagga cgacgugauc gaggugcaga agaaggacau ggagaagccc     2820

gagcucgccu acuucgucuc ccugaacacg agcuccgcca gcaucgcgcu ggugaagcac     2880

gacaacaagu ucgagacccu caccuccaac caggccaagc uguucagcaa cgccaaggag     2940

aacaacguga ucgccgaguc ccugggcauc cagaaccuca aggucuucaa gaaguacaag     3000

gugagccccc ugggggagau caaggaggcc aaguacgagc cccggcagcc gaucgcgcug     3060

aagaccuccc ccaagaagca ccgcaagcag aacaacgccc aguga                     3105


<210>  1448
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1448
augcgccggc ugggccucga ccuggggacc aacagcaucg gcuggugccu gcucgaccug       60

ggggacgacg gcgagcccgu guccaucuuc cgcaccgggg cccggaucuu cagcgacggc      120

cgcgacccga agucccuggg cagccucaag gccacgcggc gcgaggcgcg gcugacccgc      180

cggcgccggg accgcuucau ccagcggcag aagaaccuga ucaacgcccu cgucaaguac      240

gggcugaugc ccgccgacga gauccagcgc caggcccugg ccuacaagga ccccuacccc      300

auccggaaga aggcgcucga cgaggccauc gacccguacg agaugggccg cgccaucuuc      360

cacaucaacc agcggcgcgg guucaagucc aaccgcaaga gcgccgacaa cgaggcgggc      420

guggugaagc aguccaucgc cgaccuggag augaagcugg gggaggccgg cgcccggacc      480

aucggcgagu uccucgccga ccggcaggcg accaacgaca cgguccgcgc ccgccggcug      540

agcgggacca acgcccugua cgaguucuac cccgaccgcu acaugcucga gcaggaguuc      600

gacacccugu gggccaagca ggccgcguuc aaccccuccc uguacaucga ggccgcccgg      660

gagcgccuca aggagaucgu guucuuccag cggaagcuga agccccagga ggugggccgc      720

ugcaucuucc ugagcgacga ggaccggauc uccaaggccc ucccgagcuu ccagcgcuuc      780

cggaucuacc aggagcuguc caaccuggcc uggaucgacc acgacggggu cgcgcaccgc      840

aucaccgcca gccucgcccu gcgggaccac cuguucgacg agcucgagca caagaagaag      900

cugacguuca aggccaugcg cgccauccug cggaagcagg gcguggugga cuaccccguc      960

ggcuucaacc ucgaguccga caaccgcgac caccugaucg ggaaccugac cagcugcauc     1020

augcgggacg cgaagaagau gaucggcucc gccugggacc gccucgacga ggaggagcag     1080

gacagcuuca uccugaugcu gcaggacgac cagaaggggg acgacgaggu gcgguccauc     1140

cucacccagc aguacggccu gagcgacgac guggccgagg acugccugga cguccgccuc     1200

cccgacgggc acggcagccu guccaagaag gccaucgacc ggauccugcc cgugcuccgc     1260

gaccagggcc ugaucuacua cgacgcggug aaggaggccg ggcugggcga ggccaaccuc     1320

uacgacccgu acgccgcccu gagcgacaag cuggacuacu acgggaaggc gcuggccggc     1380

cacgucaugg gggccuccgg caaguucgag gacagcgacg agaagcgcua cggcaccauc     1440

uccaacccca cggugcacau cgcccucaac caggugcggg ccgucgugaa cgagcugauc     1500

cgccugcacg ggaagcccga cgaggugguc aucgagaucg gccgggaccu ccccaugggg     1560

gcggacggca agcgcgagcu ggagcgguuc cagaaggagg ggcgcgccaa gaacgagcgg     1620

gcccgcgacg agcugaagaa gcucggccac aucgacagcc gggagucccg ccagaaguuc     1680

cagcuguggg agcagcuggc caaggagccg guggaccggu gcugccccuu caccggcaag     1740

augaugagca ucuccgaccu cuucagcgac aagguggaga ucgagcaccu ccugcccuuc     1800

ucccugaccc uggacgacag cauggccaac aagaccgucu gcuuccgcca ggcgaaccgg     1860

gacaagggga accgcgcccc cuucgacgcc uucggcaacu ccccggccgg guacgacugg     1920

caggagaucc ugggccggag ccagaaccuc cccuacgcga agcgcuggcg guuccugccc     1980

gacgccauga agcgcuucga ggccgacggc ggguuccugg agcggcagcu caacgacacg     2040

cgcuacaucu cccgguacac caccgaguac aucagcacca ucauccccaa gaacaagauc     2100

ugggugguca cgggccgccu gaccucccug cuccgcgggu ucuggggccu gaacagcauc     2160

cugcgggggc acaacaccga cgacggcacc ccggccaaga agucccgcga cgaccaccgg     2220

caccacgcca ucgacgcgau cguggugggc augacgagcc gcgggcuccu gcagaagguc     2280

agcaaggccg cccggcgcuc cgaggaccug gaccucaccc ggcuguucga gggccgcauc     2340

gaccccuggg acggguuccg ggacgaggug aagaagcaca ucgacgccau caucgugagc     2400

caccgccccc ggaagaaguc ccagggcgcc cugcacaacg acaccgcgua cgggaucguc     2460

gagcacgccg agaacggcgc cagcaccgug gugcaccgcg uccccaucac gucccucggc     2520

aagcagagcg acaucgagaa ggugcgggac ccgcugauca aguccgcccu gcucaacgag     2580

accgccgggc ugagcggcaa guccuucgag aacgcggugc agaaguggug cgccgacaac     2640

agcaucaagu cccugcgcau cgucgagacc gugagcauca uccccaucac cgacaaggag     2700

gggguggccu acaagggcua caagggggac ggcaacgccu acauggacau cuaccaggac     2760

cccacgucca gcaaguggaa gggcgagauc gucucccggu ucgacgcgaa ccagaagggg     2820

uucaucccga gcuggcaguc ccaguucccc accgcccgcc ucaucaugcg gcugcgcauc     2880

aacgaccugc ucaagcugca ggacggcgag aucgaggaga ucuaccgggu gcagcgccug     2940

agcgggagca agauccucau ggcgccccac accgaggcca acguggacgc ccgcgaccgg     3000

gacaagaacg acaccuucaa gcugacgucc aagagccccg gcaagcugca guccgccagc     3060

gcccgcaagg uccacaucuc cccgaccggg cucauccggg agggcuga                  3108


<210>  1449
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1449
auggagcagc ugaacuacau ccucggccug gacaucggga ucgccagcgu gggcugggcc       60

gucguggaga ucgaccacaa cgaggacccc auccgccuga ucgacguggg gguccggacc      120

uucaacaagg cggagguggc caagaccggc gagucccuca gccugauccg ccggcuggcc      180

cgcggguccc ggcgccucgu gcggcgcaag aagaugcggc ugcugacggc caagcgccuc      240

cucaagagcg agggcauccu guccgaccuc gacuucaaca agggcgccau ccugaaccug      300

ccgaacaacu gcugggagcu ccgggucaag gggcuggaca agaagcugag cgacaaggag      360

ugggcggccg ugcuccugca caucaucaag caccgcggcu accuguccca gcggaagcac      420

gaccgcaaga ccgacgacaa ggagcucggg gcccugcuga gcggcguguc caagaaccac      480

agccuccugg aggaggggga guuccggacc gccgcggagc uggccaucaa cgucuucugc      540

aacuccaagg gccacauccg caaccagugg ggcgacuaca gccacaccuu cucccggcuc      600

gaccugcaga aggagaucaa gcagcuguuc gugagccagc gcgagcucgg gaaccccuac      660

acguccagcu ccuucgagga gaacaucauc agccugcuga ugaagcagaa ggaggccauc      720

uccggcaacg acauccucaa gaugcugggg aagugcaccu acgagaagaa cgaguacaag      780

gccgccaaga acaccuacag cgcggagcgg uucguguggc ugaccaagcu caacaaccug      840

cgcaucgucg acgacggcgu ggagcgggcc cugaacagcu ccgagcgcaa gcuccugauc      900

gacgagcccu acaagaaggc caagcugacg uacgcccagg ugcgcaagau ccucgagcug      960

cccgacagcg ccgucuucaa gggcgugcgg uacuucgcgg acaacgccga guccagcacc     1020

cugauggaga ugaaguucug gcacuccauc cgcaaggccc ucgagagcaa caaccugaag     1080

gacgaguggc uggugcucaa gaccaagccg gagcugcugg acgagaucgg gaccggcuuc     1140

uccaucuaca agacggacga cgacaucacc agcuaccugu ccaacaagcu cgacaagaag     1200

guccuggaca uccugcucgu ggaggugagc uucgacaagu ucaucgagcu gucccugaag     1260

ugccuccgga agaucauccc ccugauggag aagggguacc gcuacgacga ggccuacaag     1320

uacaucuacg gcgaggucaa caucgaguac aaccugaacg acaucaagaa ccccguggug     1380

cuccggagcu ucucccagag ccgcaagguc aucaacgccg ugauccggcu guacgggucc     1440

cccgcgcgca uccacaucga gaccgcccgg gacaucggca agagcuucaa ggagcgcaag     1500

gccaucgaga agcagcagga gaagaaccgg cuggagaagg uccgcgccgc cgagcacuuc     1560

aagcagcugu ucccgaacua cgugggcgag cccaaggcga aggacauccu gaaggugcgg     1620

cuguacgagc agcagcaggg gaagugccuc uacuccggca agauccugga caucaagcgc     1680

cugauggagc acggguacgu cgagaucgac cacgcccucc ccuucagccg gaccugggac     1740

gacuccuuca acaacaaggu gcuggugcug gccaacgaga accagaacaa gggcaacaag     1800

acgcccuacg agugguucaa cgggaaggac aacagcgaca gcuggaagcg cuuccucgcc     1860

cuggucaaca acuccggcuu cagcgcggug aagaagcagc acauccugau gaaggagauc     1920

gacgaggagg gcuucaagca gcggaaccuc aacgacaccc gcuacaucac ccgguuccug     1980

ugcaacuggg uggagaacaa caucccgcug cucgggaagg gcgucaagcg cguguucgcg     2040

ccccagggga agaucaccgc ccugcugcgc uccaacuggg gcauccggaa ggugcgcgag     2100

gacaacgacc ggcaccacgc caucgacgcc aucgucgugg ccugcacgac cgugagcauc     2160

cagcagcgca ucaccaacua cgucaagaac uccgagauca accggaagau caagcgccag     2220

aacuaccuca aguuccccga gcccuggaag uucuuccggg aggacaucag cauccgcgug     2280

uucuccaaca acccgaucga ggagcugaag cagcagcuga gcgaccggcc cgaggccaac     2340

caccaguucg ugacgccccu cuucgucucc cgcgcccccg ugcggaagau gaccgggcag     2400

ggccacgagg acaccauccg cagcgccaag cggaucuccg agggcaucag cgugcugcgc     2460

gucccgcuga ccaagcucaa cgacaagucc cuggagcuga uggugaaccg ggagcgcgag     2520

aaggagcucu acgacgcccu gaagcagcgg cugcucgagu ucaagaacga ccccgagaag     2580

gcguucgccg agcccuucua caagaagggc gggcagcagg ucaagaccgu gcgcaucgag     2640

cgggugcaga agaacggggu gcuggucaac aacaacacgg gcaucgccga caacggcaac     2700

aucgugcgcg uggacgucuu ccugaagaag aagaaguacu uccucgugcc caucuacagc     2760

uggcaggucg ccaaggggau ccugccgaac aaggccaucg uggcgcacaa gaacgagugc     2820

gacugggccg acauggacgg cuccgcccag uuccuguuca gccucuacac caacgaccug     2880

aucgaggugg ugcugaagga cuccaccauc cucggguacu ucagcagcau cgaccgggcc     2940

accgcggcca ucaucaucaa ggagcccgac auggagaagu ccaagggcaa ggaggggauc     3000

caccgcgucg gcgugaagac ggcccgcagc uucaucaagu acaacgugga cguccugggc     3060

aagaacaucu cccggugccg caaggccaag cggcugcccg ugcgcuga                  3108


<210>  1450
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1450
augcugcgca agaugcacua ccggcucgcc cuggaccugg gcagcaccuc ccucgggugg       60

gccaugaucc gccugaacgc gggcaacgag cccugcgccg ugaucaaggc cgggguccgg      120

aucuucagca acggccgcaa cccgaaggac ggguccagcc uggccgugac ccggcgcgag      180

gcccgggcga ugcgccggcg ccgggaccgc cuccugaagc ggaaggcccg caugauccag      240

acgcugaucg aguacggcuu cuuccccgcc aaggaggacc agcggaaggc ccucgagauc      300

augaaccccu ucgcgcugcg cgccaagggc cugaacgagg cccucacccc cgccgaguuc      360

gcccgggcgc uguuccacau caaccagcgc cggggguuca aguccaaccg caagaccgac      420

aagaaggaca acgacagcgg cgcccugaag accgccauca acaagcuccg ggagacgcug      480

aagaccgaga acugccgcac cgugggggag uggcugaaca agcgcgacgu cgccggccuc      540

accgugcggg cccgcuacca ccaggacaag acgaucaagg aggacgggaa guccaagauc      600

aacaaguacu acgaccugua caucgaccgg gcgaugaucg agcacgaguu cgacgagcug      660

uggaagaagc aggccgagcu caacccgacc cuguucaccg aggccgcccg cgccgaccug      720

aaggacgugc uccugcacca gcgggcgcug aagcccguca agcccggccg cugcaccuuc      780

augcccgacg aggagcgggc cccgcucgcc cugcccagca cgcagcgcuu ccggauguac      840

caggagguga acaaccugcg cauccuccgg gacggccuga aggaggaguc ccugacccuc      900

aaccagcgcg acgaccugau cgccgcccug gaggagaaca acaagcggac cuucacccag      960

aucaagaagc uccuggggcu gggcggggug gucaaguuca acuucgagga ccccaagcgc     1020

gaggagcuca agggcaacac gaccagcgcg auccugggca aggacgagca cuucgggucc     1080

gugugguuca aguucgacga ggccaagcag gacgccaucg ugaugcagcu guucaaggag     1140

gagaacgagg ccaagcuccu gcgguggcug cagaacgaga ccggcaucga cgagaagcgc     1200

gcggagacca ucgccaacgu cgggcucccc gagggcuacg ggagccugug cgcccuggcc     1260

cucgcccgga uccugccgga gcugcgccgg gacguggugc ccuacuccga cgcggccaag     1320

gccgccggcu uccaccacag cgacacgucc aacaaccagg agguccccgg ccugaccuuc     1380

cccaucgagc ucguggugac cgagaccggg gagaucaaga cgaagcacau cuuccacgag     1440

cugccguacu acggcgagua ccugcagcgc cacgucgggu ucggcagcgg gaagcccgag     1500

gacuccgccg agaagcgcua cggcaagauc gcgaacccca ccgugcacau cggccucaac     1560

caggugcggc uggucgugaa cacccugauc aagcgcuacg ggcaccccag cgaggugauc     1620

gucgaggugg cccgggaccu caagcagucc aaggaccugc gcgacgagga gggcaagcgg     1680

caggccgaga accagaagcg caacaagcgg auccgcaccg ccaucgccgg gguccugcag     1740

acgagcgagg agcgggugcg ccgggacgac aucgagaaga ugauccucug ggaggagcug     1800

agcuucgacc cggcggaccg ccggugcccc uacuccggcg ugcagaucag cgccgccaug     1860

cugcucuccg acgaggucga gaucgagcac auccugcccu ucagccagac ccuggacgac     1920

ucccucaaca acaagaccgu ggcccugcgc caggcgaacc gggugaaggg gaaccgcacc     1980

cccugggagg ccuucggcgc ccagagcacg gccggcuucg acuacgccac cauccuggcg     2040

cgggccgagc acaugccgaa ggggaagcgc uaccgguucg ccgaggacgg cuaccagcgc     2100

uggcucaagg acgacgccgg guuccugccc cgggcccuga acgacacccg ccacaugucc     2160

aaggucgcgc gcgaguacau gagccucauc ugccccaaca cccgggugau ccccggccgc     2220

augacggcca ugcugcgggg caaguucggg cugaacgacg ugcucggccu gaacggggag     2280

aagaaccgca acgaccaccg gcaccacgcc gucgacgccu gcgugaucgc cgugaccgac     2340

cagggccugc uccagcgcuu cgcgaaggcc uccgccagcg cccgggagca gcagcugaac     2400

cgccuggucg agaacaugcc gcuccccugg gaguccuacc gggagcacgu ggagcgcgcg     2460

aucgacgcca ucugggugag ccacaagccc gaccacuccc acgagggggc caugcacaac     2520

gacaccgccu acggccugcu gggcgacggg aaggucagca uccacaagau gguggagggc     2580

aagcgggagc gcaucgagga gucccucaag gugaucgccu ucaccaacgc gaaggccaac     2640

gaccggcacg ggcugcugga caacggcgag ccgcgccccu acaaggggua caagggcgac     2700

agcaacuacu gcaucgagau cauccggaac gagaagggca agugggaggg ggaggucauc     2760

uccaccuucg acgccuacca gcucgugcgc gagcacggcg uggcccggcu gcgccacccc     2820

acgcugagcg ucuccgggaa gccccucgug augcggcuga ugaaggacga gaccauccgc     2880

auggugacca acgagaagcc gaucaccgcc cgcgucugcu gggugcggag cgacacgcgc     2940

aucgcguucg ccagcaccuc cgaggccaac guggacgccc gggaccgcga caagaacgac     3000

agcuucuccu acaucgucaa gaccgccagc gugcugcaga agcuccaggc gcggcgcauc     3060

uccaucagcc ccaccggcga gcugcgggac cccggguuca aggaguga                  3108


<210>  1451
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1451
auggagcagc ugaacuacau ccucggccug gacaucggga ucgccagcgu gggcugggcc       60

gucguggaga ucgaccacaa cgaggacccc auccgccuga ucgacguggg gguccggacc      120

uucaacaagg cggagguggc caagaccggc gagucccuca gccugauccg ccggcuggcc      180

cgcggguccc ggcgccucgu gcggcgcaag aagaugcggc ugcugacggc caagcgccuc      240

cucaagagcg agggcauccu guccgaccuc gacuucaaca agggcgccau ccugaaccug      300

ccgaacaacu gcugggagcu ccgggucaag gggcuggaca agaagcugag cgacaaggag      360

ugggcggccg ugcuccugca caucaucaag caccgcggcu accuguccca gcggaagcac      420

gaccgcaaga ccgacgacaa ggagcucggg gcccugcuga gcggcguguc caagaaccac      480

agccuccugg aggaggggga guuccggacc gccgcggagc uggccaucaa cgucuucugc      540

aacuccaagg gccacauccg caaccagugg ggcgacuaca gccacaccuu cucccggcuc      600

gaccugcaga aggagaucaa gcagcuguuc gugagccagc gcgagcucgg gaaccccuac      660

acguccagcu ccuucgagga gaacaucauc agccugcuga ugaagcagaa ggaggccauc      720

uccggcaacg acauccucaa gaugcugggg aagugcaccu acgagaagaa cgaguacaag      780

gccgccaaga acaccuacag cgcggagcgg uucguguggc ugaccaagcu caacaaccug      840

cgcaucgucg acgacggcgu ggagcgggcc cugaacagcu ccgagcgcaa gcuccugauc      900

gacgagcccu acaagaaggc caagcugacg uacgcccagg ugcgcaagau ccucgagcug      960

cccgacagcg ccgucuucaa gggcgugcgg uacuucgcgg acaacgccga guccagcacc     1020

cugauggaga ugaaguucug gcacuccauc cgcaaggccc ucgagagcaa caaccugaag     1080

gacgaguggc uggugcucaa gaccaagccg gagcugcugg acgagaucgg gaccggcuuc     1140

uccaucuaca agacggacga cgacaucacc agcuaccugu ccaacaagcu cgacaagaag     1200

guccuggaca uccugcucgu ggaggugagc uucgacaagu ucaucgagcu gucccugaag     1260

ugccuccgga agaucauccc ccugauggag aagggguacc gcuacgacga ggccuacaag     1320

uacaucuacg gcgaggucaa caucgaguac aaccugaacg acaucaagaa ccccguggug     1380

cuccggagcu ucucccagag ccgcaagguc aucaacgccg ugauccggcu guacgggucc     1440

cccgcgcgca uccacaucga gaccgcccgg gacaucggca agagcuucaa ggagcgcaag     1500

gccaucgaga agcagcagga gaagaaccgg cuggagaagg uccgcgccgc cgagcacuuc     1560

aagcagcugu ucccgaacua cgugggcgag cccaaggcga aggacauccu gaaggugcgg     1620

cuguacgagc agcagcaggg gaagugccuc uacuccggca agauccugga caucaagcgc     1680

cugauggagc acggguacgu cgagaucgac cacgcccucc ccuucagccg gaccugggac     1740

gacuccuuca acaacaaggu gcuggugcug gccaacgaga accagaacaa gggcaacaag     1800

acgcccuacg agugguucaa cgggaaggac aacagcgaca gcuggaagcg cuuccucgcc     1860

cuggucaaca acuccggcuu cagcgcggug aagaagcagc acauccugau gaaggagauc     1920

gacgaggagg gcuucaagca gcggaaccuc aacgacaccc gcuacaucac ccgguuccug     1980

ugcaacuggg uggagaacaa caucccgcug cucgggaagg gcgucaagcg cguguucgcg     2040

ccccagggga agaucaccgc ccugcugcgc uccaacuggg gcauccggaa ggugcgcgag     2100

gacaacgacc ggcaccacgc caucgacgcc aucgucgugg ccugcacgac cgugagcauc     2160

cagcagcgca ucaccaacua cgucaagaac uccgagauca accggaagau caagcgccag     2220

aacuaccuca aguuccccga gcccuggaag uucuuccggg aggacaucag cauccgcgug     2280

uucuccaaca acccgaucga ggagcugaag cagcagcuga gcgaccggcc cgaggccaac     2340

caccaguucg ugacgccccu cuucgucucc cgcgcccccg ugcggaagau gaccgggcag     2400

ggccacgagg acaccauccg cagcgccaag cggaucuccg agggcaucag cgugcugcgc     2460

gucccgcuga ccaagcucaa cgacaagucc cuggagcuga uggugaaccg ggagcgcgag     2520

aaggagcucu acgacgcccu gaagcagcgg cugcucgagu ucaagaacga ccccgagaag     2580

gcguucgccg agcccuucua caagaagggc gggcagcagg ucaagaccgu gcgcaucgag     2640

cgggugcaga agaacggggu gcuggucaac aacaacacgg gcaucgccga caacggcaac     2700

aucgugcgcg uggacgucuu ccugaagaag aagaaguacu uccucgugcc caucuacagc     2760

uggcaggucg ccaaggggau ccugccgaac aaggccaucg uggcgcacaa gaacgagugc     2820

gacugggccg acauggacgg cuccgcccag uuccuguuca gccucuacac caacgaccug     2880

aucgaggugg ugcugaagga cuccaccauc cucggguacu ucagcagcau cgaccgggcc     2940

accgcggccc ugaucaucaa ggagcccgac auggagaagu ccaagggcaa ggaggggauc     3000

caccgcgucg gcgugaagac ggcccgcagc uucaucaagu acaacgugga cguccugggc     3060

aagaacaucu cccggugccg caaggccaag cggcuccccg ugcgcuga                  3108


<210>  1452
<211>  3111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1452
augcgcaucu ucggcuucga caucgggauc accagcaucg gcugggccua cguggagucc       60

aacgagcuga aggacugcgg gguccggauc uucaccaagg ccgagaaccc caagaacggc      120

aagagccucg cgcugccgcg ccgggaggcc cgcggggccc ggcgccggcu ggcccgccgg      180

aaggcccgcc ucaacgcgau caagcggcug cugugcaagg aguucggccu caacgugucc      240

gacuacuucg ccaacgacgg cgagcugccc aaggccuacg agacgaccaa ggacaccaag      300

agccccuacg agcugcgcuc ccucgcccug gagcagaagc uggagcccaa ggagcucgcg      360

cgggugaucc ugcacaucgc caagcaccgc ggguacggca acaagcacgc caagaccggg      420

agcgacaagg acuccggcaa gguccugagc gccaucaagg ugaacgagac ggccaugaag      480

gacaaguacc aguccguggg ggaguaccuc uacaaggagu ucuaccagaa gcugcggcug      540

gagagcgaga agaagggcaa caacaucacc aaggaguuca agaacguccg caacaagggc      600

gagaacuacg agcacugcgu ggcgcaggag cagguccggg ccgagcucga gcugaucuuc      660

gccaagcaga aggacuucgg ggccaccuuc uccgagaacu ucgagagcca gaucaucgag      720

accgccuucu accagcgcga ccugaagucc uucgaggaca aggugggcaa gugcguguuc      780

uacgagaacg agaaccgggc gccgaaggac agccucuccg ccauggaguu cguggcccug      840

acgcgcauca ucaacacccu gaagaaccuc gaggagaagu ccaagaaccu ggggaucggc      900

gaggucuacg gcaaggacaa gauccaggag auccugaaga ucgugcucga caagggggag      960

augagcuaca agaagcugcg ggagauccug caccucgacg agcacauccu guucgccaag     1020

gacagcaagc uggacuacac ccagggcgug caggaggccg agaaggcgaa guucaucgag     1080

cucaagaacc ugaaggccuu caagaaggcc augggcgggg acuucuccaa cuucgcccgc     1140

gaggagcugg acaagaucgc caccgacauc acgcucguca agagcaagga gaaccuggcg     1200

aagaagcugc agaacuacag cgcccucucc aaggagcagg ucgaggcccu gagcaaccug     1260

uccuucgcca agcacaucga ccucagccug aaggcgcugg agcagauccu ccccuucaug     1320

cgcggggaca agaccggcaa gugccugcgg uacgacgagg ccgugaagaa ggccgagcug     1380

caggagcacc gcaagcacaa gcagaagggc gaguuccuca ucccccugaa ggacuacgag     1440

cccuaccugg ccaacccggu gguggcccgg gcgcuguccg aguaccgcaa ggugcucaac     1500

gcccugcugc ggaaguacgg gaagguccac aagauccacc ucgaguucac ccgcgaggcc     1560

aagcugagcg ccaaggagcg gcagaaguac gagaaggagc agaaggagca cuucgaggcc     1620

aaccagcgcg cgauccagca gugcgagauc cugggccucc ccaucaacgg gaccaacauc     1680

cugaagauga agcuguggcu cgagcagggc gagugcugcg ccuacuccgg ggagaagauc     1740

acgcgggagc accuccugga cagcaacgcc cuccaggugg accacaucua ccccuacucc     1800

cgcagcuucg acgacuccua caugaacaag gugcuggucu ucgccaagga gaaccaggac     1860

aagggcaaca agacccccuu cgaggccuuc ggcggggaca aggagaagug ggaccggauc     1920

cugagccugu ccgcgaagcu gccgaagcag aagcagcgcc ggaucagcaa caccaacuuc     1980

aaggacaagg agggcggguu ccuggcccgc aaccucaacg acaccggcua caucgccaag     2040

cuggccuccc aguggacgaa ggacugccug gaguucgagg accuccggga gaacgaggug     2100

accaucgcgg gggagaaggg cagcuccguc cacaucgagg uggugagcgg ccagcugacc     2160

uccaugcugc gccacuacug ggggcucggc aacaaggacc ggaacaacca ccuccaccac     2220

gccgucgacg ccgugauccu ggccuacacc aacaacgcca ugaucaaggc guucgccaac     2280

uuccgcaaga accaggagga gaacaagaag aaguucuacg ccaagcagau cgccgagagc     2340

gaguacaagg ccaagaaggc guucuucgag cccugcaaga acuuccggca gcgcguggag     2400

gagaagauca aggagaucuu cgucagcaag ccgccccgca agcgggugcg cggggcccug     2460

caccaggaga cguucuaccc cuucgagaag cugugcaacg acaaggagua cuccgagaag     2520

cugaagaugg acuacggcgg cgagaagggg augcagaagg ccaucgcccu cggcaagauc     2580

cggcagaucg ggaccaagau cgugagcaac ggcgccaugg uccgcgugga caucuucaag     2640

gacaagaagg ggaaguucua cgcggugccc aucuacacca uggacuucgc ccugggcauc     2700

cugcccaaca aggccgucgc caucggcaag gacaaggacg gggugaucaa ggacuggauc     2760

gagauggacu ccaacuacga cuucugcuuc agccucuuca aggacgaccu gauccuggug     2820

cagaagaagg agauggagcg ggcggagcuc ugcuacuucg ucuccuucgg cagcaccaac     2880

gcccagauca acguggagaa gcacaacaac aaguucgagg ggcuguccga gaacgagaag     2940

auccuguaca gcaacgccac gcagaaggag guggccggca aguccaucgg gauccagaac     3000

cucaagaccu ucgagaagua ccaggucagc ccccugggcg aggugcagaa ggccgaguuc     3060

gagccgcacc aggagaucaa gcugaagucc agccccaagc gcaaggacug a              3111


<210>  1453
<211>  3111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1453
augcugcaca agaugcacua ccgccucgcc cuggaccugg gcagcaccuc ccucgggugg       60

gccaugaucc ggcuggacgc ggacaaccag cccugcgccg ugaucaaggc cggcguccgc      120

aucuucagca acgggcggaa cccgaaggac ggcuccagcc uggccgugac ccgccgggag      180

gcccgcgcga ugcggcgccg gcgcgaccgg cuccugaagc gcaaggcccg gaugaugcgc      240

acgcugaucg aguacggguu cuuccccgcc gaggccgcgc agcggaaggc ccucgagacc      300

aucaaccccu ucgcccugcg cgccaagggc cuggacgagg cccucacccc cgcggaguuc      360

ggccgggccc uguuccacau caaccagcgc cggggguuca aguccaaccg caagaccgac      420

aagaaggaca acgacagcgg cgcccugaag acggccaucg ggaaggugcg ggccaccauc      480

gaggcggagg gcugccgcac cgucggggag uggcuccaca agcgcgacgu ggccggccug      540

accgugcggg cccgcuacca ccaggacaag gucaucaagg aggacggcaa gacgaagauc      600

gacaaguacu acgaccugua caucgaccgg gccaugaucg agcaggaguu cgacgagcuc      660

uggaagaagc aggccgagcu gaacccggcg cuguucaccg aggccgcccg cgccgaccuc      720

aaggacgugc ugcugcacca gcggccccuc aagcccguga aguccgggcg cugcaccuuc      780

augcccgacg aggagcgggc cccgcuggcg cugcccagca cccagcgcuu ccggauguac      840

caggagguca acaaccuccg cauccugcgg gacggccuga aggaggagcc ccucacgcug      900

aagcagcgcg acgaccugau cgccgcccuc gagaagaacu ccaagcacag cuucacccag      960

aucaagaccc ugcugggggu gggcggcgcc gugcaguuca acuucgagga ccucaagcgg     1020

caggagcuga aggggaacac cacguccgcg auccugagca acgacaagca cuucggcaag     1080

gccugguuca ccuucgacga ggccaagcag gacgccaucg uccuccagcu ggugaaggag     1140

gagaacgagg ccaagcuggu gcgcuggcuc caggacgaga ccgggaucga cgagaagcgg     1200

gcggaggcca ucgccaacgu cggccugccc gagggguacg gcucccugug cgccaaggcc     1260

cucgcgcgca uccugccgga gcugcggcgc gacgugauga ccuacgacaa ggccgugcag     1320

gccgccggcu ucgagcacca cagccggcuc ggguccaaca cggagauccc cggccugacc     1380

uucaagaucg agagcaucga ccaggacucc ggggagguca agcaguucca cauccacaag     1440

gugcugcccu acuacggcga guaccugcag cgccacgugg gguucggcag cggcgagccc     1500

gaggacccgg ccgagaagcg cuacgggaag aucgcgaacc ccaccgucca caucggccuc     1560

aaccagaucc ggcugguggu gaacgcccug cucaagcgcu acgggcaccc cuccgagguc     1620

aucguggagc uggcccggga ccugaagcag agcaaggacc agcgcgacga ggagagcaag     1680

cggcaggccg agaaccagaa gcgcaacaag cggcuccgcg acgcgaucgc cggcacccug     1740

aagaucuccg aggagcgggu ccgccgggac gacauccaga agaugauccu gugggaggag     1800

cucagcuucg accccgccga ccgccggugc ccguacuccg gggcccagau cagcgccacg     1860

augcugcugu ccgacgaggu ggagguggag cacauccucc ccuucagcca gacccuggac     1920

gacucccuga acaacaagac cgucgcgcuc cgccaggcca accggaucaa gggcaaccgc     1980

acccccuggg acgccuucgg cgcccaggcc guggcggggu ucgacuacga gagcauccug     2040

gugcgggccg agggcaugcc caaggccaag cgcuaccggu ucgccgagaa cggguaccag     2100

cgcuggcuga aggacgacgc cuccuuccuc gcgcgggccc ugaacgacac gcgccacaug     2160

agcaaggucg ccuucgagua ccugaagcuc aucugcccga acacccgcgu gauccccggc     2220

cggaugaccg ccaugcugcg cgcgaaguuc ggccugaacg acgugcucgg gcugaacggc     2280

gagaagaacc ggaacgacca ccgccaccac gccgucgacg ccugcgugau cgccgugacc     2340

gaccaggggc ugcuccagcg guucgccaag gcguccgcca gcgcccgcga gcagcagcug     2400

aaccggcugg ucgagaacau gccccucccc uggggcuccu accgcgagca cgugcagcgg     2460

gccaucaacg ccaucuggau cagccacaag ccggaccacu cccacgaggg ggcgaugcac     2520

aacgacacgg ccuacggccu gcgcggcgac gggaagguga gcuuccacaa gauggucgag     2580

ggcgugcgga cccacuccga ggacaaccug aaggugaucg agaucagcaa cgccaaggcc     2640

accgaccgcc acgggcuccu gcccaacggc gagccccggc cguacaaggg guacaagggc     2700

gacuccaacu acugcaucga gaucguccgc aacgagaagg gcaaguggga gggggaggug     2760

aucagcaccu ucgaggccua ccagcuggug cgggagcacg gcaucgcgcg ccuccggcac     2820

agcacgcugu ccgucagcgg gaagcugcuc gugaugcgcc ugaugaucga cgacgugguc     2880

cggcugauca ucgacgagca gacccgcacc augcgcaucg ccacccucuc cggcaacggg     2940

cagaucuuca uggccgacac cagcgaggcc aacguggacg cccggaaccg cuccaaggag     3000

gagacguucg cguacgugag caagauggcc ggcucccugc agaaggccaa cgcccggcgc     3060

aucaacauca gccccaucgg cgagcugcgg gaccccgggu ucaaggagug a              3111


<210>  1454
<211>  3114
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1454
auggagcgca ucuucggcuu cgacaucggg accaccagca ucggcuucuc cgugaucgac       60

uacagcucca cgcagagcgc cgggaacauc cagcggcugg gcguccgcau cuuccccgag      120

gcccgggacc cggacgggac gccccucaac cagcagcgcc ggcagaagcg caugaugcgg      180

cgccagcugc ggcgccggcg cauccggcgc aaggcgcuga acgagacccu ccacgaggcc      240

ggcuuccugc ccgccuacgg cuccgccgac uggcccgugg ugauggccga cgagccguac      300

gagcugcggc gccgggggcu cgaggagggc cugagcgcgu acgaguucgg gcgcgccauc      360

uaccaccugg cccagcaccg gcacuucaag ggccgcgagc ucgaggaguc cgacaccccc      420

gaccccgacg ucgacgacga gaaggaggcc gcgaacgagc gggccgccac ccugaaggcc      480

cugaagaacg agcagaccac ccucggggcc uggcuggcgc gccggccccc gagcgaccgg      540

aagcgcggca uccacgccca ccggaacgug guggccgagg aguucgagcg ccugugggag      600

guccagucca aguuccaccc cgcccucaag agcgaggaga ugcgggcccg caucuccgac      660

accaucuucg cgcagcggcc cguguucugg cgcaagaaca cgcugggcga gugccgguuc      720

augcccgggg agccgcugug ccccaagggc agcuggcucu cccagcagcg ccggaugcug      780

gagaagcuga acaaccucgc caucgccggg ggcaacgccc gcccccugga cgccgaggag      840

cgggacgcga uccugagcaa gcuccagcag caggccucca ugagcuggcc cggcgugcgc      900

uccgcccuga aggcccugua caagcagcgc ggggagccgg gcgccgagaa gucccucaag      960

uucaaccugg agcucggggg cgagagcaag cugcugggga acgcgcugga ggccaagcuc     1020

gccgacaugu ucggccccga cuggcccgcc cacccccgca agcaggagau ccggcacgcg     1080

guccacgagc gccugugggc cgccgacuac ggcgagaccc cggacaagaa gcgggugauc     1140

auccugagcg agaaggaccg caaggcccac cgcgaggccg cggccaacag cuucguggcc     1200

gacuucggga ucaccggcga gcaggccgcc cagcuccagg cgcugaagcu gcccaccggg     1260

ugggagcccu acuccauccc cgcccucaac cuguuccugg ccgagcucga gaagggcgag     1320

cgguucgggg cccuggucaa cggcccggac ugggagggcu ggcgccggac gaacuucccc     1380

caccgcaacc agcccaccgg ggagauccug gacaagcucc ccagcccggc cuccaaggag     1440

gagcgggagc gcaucagcca gcugcggaac cccaccgugg ugcgcaccca gaacgagcug     1500

cggaaggucg ugaacaaccu caucggccug uacgggaagc ccgaccgcau ccggaucgag     1560

gugggccgcg acgucgggaa guccaagcgg gagcgcgagg agauccagag cggcauccgg     1620

cgcaacgaga agcagcggaa gaaggcgacg gaggaccuga ucaagaacgg caucgccaac     1680

ccgucccgcg acgacgugga gaaguggauc cuguggaagg aggggcagga gcggugcccc     1740

uacaccggcg accagaucgg guucaacgcc cucuuccgcg agggccggua cgagguggag     1800

cacaucuggc cccgcagccg cuccuucgac aacagccccc ggaacaagac ccugugccgc     1860

aaggacguca acaucgagaa gggcaaccgg augccguucg aggccuucgg gcacgacgag     1920

gaccgcuggu ccgcgaucca gauccggcug cagggcaugg ugagcgccaa gggcgggacc     1980

gggauguccc ccggcaaggu gaagcgcuuc cucgccaaga cgaugcccga ggacuucgcc     2040

gcccggcagc ugaacgacac ccgcuacgcg gccaagcaga uccuggccca gcucaagcgg     2100

cuguggcccg acaugggccc ggaggccccc gucaaggugg aggccgucac cggccaggug     2160

accgcgcagc ugcgcaagcu cuggacgcug aacaacaucc uggccgacga cggggagaag     2220

acccgggccg accaccgcca ccacgccauc gacgcccuca ccguggcgug cacccacccc     2280

gggaugacga acaagcugag ccgguacugg cagcugcgcg acgacccccg ggccgagaag     2340

ccggcccuca ccccgcccug ggacaccauc cgcgccgacg cggagaaggc cgucuccgag     2400

aucgugguga gccaccgggu ccgcaagaag guguccggcc cccugcacaa ggagaccacg     2460

uacggggaca ccggcaccga caucaagacc aagagcggca cguaccggca guucgugacc     2520

cgcaagaaga ucgagucccu gagcaagggg gagcucgacg agauccgcga cccccggauc     2580

aaggagaucg ucgccgccca cguggccggc cgcgggggcg accccaagaa ggcguucccg     2640

cccuaccccu gcgugagccc cgggggcccc gagauccgga agguccgccu gaccuccaag     2700

cagcagcuga accucauggc ccagaccggc aacggguacg ccgaccuggg cagcaaccac     2760

cacaucgcca ucuaccggcu gcccgacggg aaggccgacu ucgagaucgu gucccucuuc     2820

gacgcgagcc gccggcuggc ccagcgcaac ccgaucgugc agcggacccg cgccgacggc     2880

gccuccuucg ucaugagccu ggccgcgggg gaggccauca ugauccccga gggcuccaag     2940

aagggcaucu ggaucgugca gggggugugg gccagcggcc aggucgugcu cgagcgggac     3000

acggacgccg accacuccac caccacccgc cccaugccga accccauccu gaaggacgac     3060

gccaagaagg ugagcaucga ccccaucggg cggguccgcc ccuccaacga cuga           3114


<210>  1455
<211>  3117
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1455
auggccaagc agguccgcua ccggcugagc cucgaccugg gcuccaccag ccuggggugg       60

gccaucuucc gccucgacgc ggacgcccac cccaccgcca ucaucaaggc cggcgugcgg      120

aucuucuccg acgggcgcaa cgccaaggac ggcagcuccc uggcggugac gcggcgcgcc      180

gcccggagca ugcgccggcg ccgggaccgc cugcuccggc gcaagacccg gaugcuggac      240

gcccugcucg aguacggguu cuucccgcag gacgcggccc agcgcaaggc ccuggagcgg      300

cugaaccccu acgcccuccg cgccgagggc cuggcgcggc cccugacccc cggcgaguuc      360

gcccgcgcca ucuuccaccu caaccagcgg cgcggguuca aguccaaccg caagaccgac      420

aagaacgaca acgacagcgg cgcccugaag acggccaccu cccagcugcg gcaggacauc      480

caggcgagcg ggugccgcac cgugggcgag uggcucugga cccagcggcu gcagaagaag      540

ccggaggggg ucccccacca gggcgugcgc gcccgguacc gcgagacgcc ccacaucaac      600

uccgagggca agaagcggau cgagaagaag uacgaccugu acaucgaccg cagcaugauc      660

gaggccgagu ucgacgcccu cugguccgcc caggcgaccu ucaaccccgu ggucuuccac      720

gccaccgccc acgagaagcu gaaggacauc cugcucuacc agcggccgcu gcgccccgug      780

aagcccgggc ggugcacccu gcuccccgag gaggagcgcg ccccgcuggc ccugcccagc      840

acgcagcggu uccgcaucua ccaggagcuc aacaaccugc ggcugcucgu ggaccagcug      900

aacgagcagc cccugacccu cgcgcagcgc gacaccaucg ccacccugau ggagggccgg      960

gccaagaucu ccuucgacca gauccgcaag gcccugaagc ucgggagcau gacgaaguuc     1020

aaccuggagg acgacaagcg gaacgagcug aagggcaacg cgaccggcuc ccagcucgcc     1080

aagaaggagu ucuucgggcc cgccuggcac caguucgacg aggcccugca ggacgacauc     1140

gucuggcacc uccugagcga ggagaacgag uccgcccuga ucagcuggcu gcaggaccgc     1200

accggcaucg acgcggccac cgcccaggcc gugagcaacg ccucccuccc ggagggguac     1260

ggcagccugu cccgcaaggc gcuggaggug auccugcccc ggcuccgcag cgaggucauc     1320

acguacgaca aggccgugca ggccgccggg uucucccacc acagccagcu gggcuucgag     1380

uacgaccccg acgacgugga ggacuggauc gaccacgcca ccggcgaggu caagcccauc     1440

uucaagcggc ugccguacua cgggaaggcg cuccagcgcc acguggccuu cggcuccggg     1500

aagcccgagg acagccagga gaagcgguac ggcaagaucg ccaaccccac cgugcacauc     1560

gggcugaacc agguccgcgu gguggccaac gcgcugaucg cccgguacgg ccgccccacc     1620

gagaucauca ucgagcucgc ccgggaccug aagcaguccc gcgagcagcg gcagcaggac     1680

cagcgccggc aggccgacaa ccagaagcgc aacgcccgga uccgcaacag caucgcgcag     1740

gcccugggca ucgccgaggc ccggguccgc cgggaggaca uccagaagug gauccucugg     1800

gaggagcugg acccggggga cgccaugcac cgcugcugcc ccuacuccgg cgugcccauc     1860

agcgcgucca ugcugcucag cgagcagguc gagaucgagc acauccugcc cuucuccaug     1920

acgcuggacg acagccucaa caaccggacc gugucccugc gccaggccaa ccgcaucaag     1980

cggaaccgca gcccguggga cgcccgggag gacuucgagg cccaggggug gcacuacgac     2040

ggcauccugc agcgcgccgg gcucaugccc gugcagaagc gguaccgcuu cgcggccgac     2100

ggcuaccagc gguggcugcg cgacgaccag gacuuccugg cccgggcccu caacgacacc     2160

cgcuaccugu cccggaucgc ggccgaguac cugcgccucg ucugccccca gggcacccgg     2220

gugaucccgg ggcagcugac ggcccugcuc cgccggcgcu ucggccugaa cgacauccug     2280

gggcucgagg gcgagaagaa ccggaacgac caccgccacc acgccgugga cgccugcguc     2340

aucggcauca ccgaccacgg gcugaugcag cgguucgcgc gcgccagcgc ccaggcccgc     2400

gagcagggcc ugacccggcu cgccgaggag augccccugc ccuggcccac cuaccgcgac     2460

cacguggacc gggcggugag ccugaucugg gucucccaca agccggacca cgggcacgag     2520

ggcgccauga uggaggagac ggccuacggg cucaugaagg acggcaccau ccgccagcgg     2580

cacaaggccg acggcaccaa ggggcgcgag guggccaacg ugauccggau cgcggagccc     2640

gcccagccca cccgccacgg cgucgacgac caggggcggc cccugccgua caagggcuac     2700

aucgggggca gcaacuacug caucgagauc acgcgcggcg cccaggggaa gugggagggc     2760

accgugaucu ccaccagcga cgccuacggg aucgugcggg cccgcggcau cgcggggcug     2820

cggaacaccu cccagggcca gaacggccac ccccucguca ugcgccuggu gaucggggac     2880

agccugcggc uccgcaucaa cgacgcccug cagaccaugc gggugacgaa gaucuccggc     2940

aacgggcagg ucuucauggc cggcgugcac gaggccaacg uggacgcgcg caaccgggac     3000

aagagccagg ccuucgcccu ggucuccaag auggccggga gccuccaggc cgcgcaggcc     3060

cgccaggcca ccaucucccc caucggcgag auccgggacc ccggcuuccc gggguga        3117


<210>  1456
<211>  3120
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1456
augaagaagc gcauccuggg ccucgacauc gggaucgcca gcgugggcug ggcccugauc       60

gaguucgacg acgaguucca ggacaacaag aagaagggga ccaucaucaa guccggcguc      120

cggaucuuca cccgcgcgga gacgcccaag gagcgggagc cgcuggccaa gccccgccgg      180

gaggcccgca gccagcggcg ccggaucucc ugccgcgugg agcggcucaa cuucgugcgc      240

gagcuguucg ucaagaacgg gcugguggcc aaggagagcg ugaacaagaa caaccccaac      300

aacaucuaca ucacccucaa ccagaucaag acccccuggc agcugcggau ggaggcccug      360

gaccgcaagc ucaccaacga ggaguucgcg gucgugcuga cgcaccuggc caagcaccgg      420

ggcuacaagu cccuccgcaa gaccgacagc aaggacaaca agaccggcga gaugcugaag      480

gccaucaacc agaacaagga gcggcuggac aacuccgacu accgcacccu cggggagaug      540

uucugcaagc acgagagcua caaggagcac aagcggaaca aggacggcga guacaucaac      600

uccgugacgc gcgagcugau cgaggaggag gccgagaaga ucuucgcgaa gcagcggaag      660

gaggggaaca acuucgccga cgucgacauc gagcgcgagu accugaagau cgccuucagc      720

cagaagccga ccggcuccau caacaagaug gugggguucu gcaccuucga gaacggcgag      780

aagcgcgcgc cccgggacag cuacuccgcc gagcuguuca aggugcucaa caccaucaac      840

aacacgacca ucaccgacau gaagaccggc gagacgcgca acuucaccgg ggccgagauc      900

aagaagauca ucgagaucau caaggacacc aagggcauca ccuacgucaa gaucaagaag      960

gagcugaagc ucgggagcuc ccaguucagc ggcguggacu accgggagaa cgccaagucc     1020

aagggcgagg gggagaucuu cgugagcaug aagacguacc acgagaucaa guccaagauc     1080

gagaagcuga gcaaggagaa guggaacaag cugaaggaga acaccgagcu ccuggacagc     1140

aucgucaaca ucaucgccaa cuucaaggac gacgagucca ucaagaagga guuccagaag     1200

cugaagaucg acgaggacau caucgacgug cuccugaacu ucgaguucaa gaaguucauc     1260

aaccugagcu ucaaggcccu ccgcaagauc auccccuucc aguuccaggg ccagaaguac     1320

aucaccgccu ccaugagcgu gggguacucc cugaagcagg agggcaucaa gaacaaguuc     1380

cugcgggucc ccagccagga ggagaacgug aacgugccgg ucguggcgcg cgccuucgcc     1440

cagacccgga aggugaucaa cgccgucauc cgcgaguacg ggcaguucga ccagaucaac     1500

aucgagcucg ccacggaccu gaagaacucc aaguucgacc ggagcaagau cgagaagggc     1560

cagaaggagu uccaguccca gaaggacaag guguuccagg agguccagga ccugaccggc     1620

gagaucaucc ccagcuccag ccuccugcug aaguaccgcc ucuggcggga gcaggacgag     1680

cgcugcaucu acuccgggcg gaagaucagc cgcaaggacc ugcuggacac cggcuuccuc     1740

gacaucgacc acaucauccc cuacuccaag agcauggacg acggguucaa caacaaggug     1800

cugugccugg cggaggagaa ccggaacaag aagaacgaca uccccuucaa cuacuaccag     1860

cgcaucggcc gggacuggaa cuuccucauc uccaucaucg ccagcaugaa gaacaugaag     1920

uacgugaaga agaagcgccu gcugaagcag gagcucuccg acaucgagau cgagagcuuc     1980

aucgagcgga acaucaacga cacccgcuac gccacgcgcu acaucaagaa cuuccuggag     2040

aacaaccugg aguucaagga gaacgaguuc aucaaggcca agguccaggc ccggcacggg     2100

ggccugacca gcgugcuccg cuacaacugg ggcaucucca agaaccggga ggagagcuac     2160

cugcaccacg cgaaggacgc caucaccgug gccugcucca cccaggggau gguccaguac     2220

aucagcaaca ucucccagaa guacgagaac ggcaaguaca agaugaagca gaucaaggag     2280

cgcacggaga accggauccc gaagcccugg gacagcuuca aggacgacgu ggagcgcucc     2340

aucaacgaca ucuucgugag cuucgcgccc cggcacaagg ucaccggguc cgcccacaag     2400

gagaccaucu acagcaagaa gcaccuggag aagggcuacg ugaccaucaa gaagucccuc     2460

gacaagauca agcugagcga ccugaagaac auccccugcg acgagaacug cgacaucauc     2520

cgcgugcuca aggagcggcu ggagaaguuc aacgacgacu cccugaaggc cuucgccgac     2580

ccgguccaca ugcccacgaa gugcagcaac aagaaggggc ccaucauccg cuccgugaag     2640

aucaaggaga acaacaagac cggcaucgag gugcggaagg gccucgucga gcgcggggac     2700

auggugcggg uggacgucuu caucaaggac aagaaguacu accuggugcc caucuacgug     2760

agcgacuuca agaacaagga ccugccgaac aaggccaucg ucuccagcaa gagcgagaac     2820

gaguggauca ugaucgacaa gaccuacucc uucaaguuca gccucuucaa ggacgaccug     2880

aucaagauca agaacaagaa gggcgagauc uucggguacc ugggcggcgu gcaccgccuc     2940

accgggggca ucaacaucaa guccugcuac aagcccaagg agaaggacgg gaacaucggc     3000

agcaagugcc ugcuggacuu ccggaaguuc caggugggga uccucggcga cuacaacgag     3060

gucaagcacg agaagcgcau gcccgccuac aucaacacca agaaggacaa ggagcacuga     3120


<210>  1457
<211>  3123
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1457
auggaccgcc gggacgcccg cgccaugcgg cgccggcgcg accgguucca gcagcgccag       60

cgggcgcugc ucaaguaccu gguggccgac ggccuguucc ccgaggacga gggggagcgc      120

aaggcccucg ccgcccugga cccguacgcg cugcgggccc gcgcccucga cgaggcccug      180

agccugcacg agcucggccg ggcgcuguuc caccugaacc agcgccgggg guucaagucc      240

aaccgcaagg ccgaccggaa ggccgacgac gacgccggca agaucgccau cggggucgac      300

cgcgcgcggg aggccauggc cgagaacggc gcccgcaccu acggcgagug gcuccaccgg      360

cgccgcgcca gcgcgaccga cgccaacgcc aucccgcccg ugcggacgcg ccugcggccc      420

gaguccgggg aggacgccaa gggcgacggg uacgacuucu accccggccg cgaccugcuc      480

gaggaggagu ucgccgcgau cugggaggcc caggccgccc accaccccga cgugcugacc      540

gacgacgucu acgagcggcu guucgagauc guguucuacc agcgcccccu caaggcgccc      600

cagaucggga ugugcacccu gaucgccggc gaccggcgcc ugccgaaggc ccacccccuc      660

uuccagcggc gccggcugcu ggaggagcuc aacgcccuga ugaucgugcg ccccggcgag      720

gucgcccagc ggcugacccg cgaggagcgg gacacccucu uccugaagcu gaaggaccgc      780

aacaagguga gcuucgaguc ccuccggaag acgcugaagc uggaccccga cgcgcgcuuc      840

aacaaggaga gcgagaaccg gaccgagcuc aagggggacg agguggccgc cgccaugggc      900

uccaagaccc gcuucgggaa ccgcuggguc caccugagca ucgaggagca gugggccgug      960

aucgagcggc ugggcgacgu ggaguccgac gcggaggacg ccgccuuccg cgccuggcuc     1020

gccgagcggu acgagcugac gccggagcag acccgcgcgg ucaccaacgc gccccugccc     1080

cagggcuacg ggcgguucgg cgagaccgcc acgcgcaagc ucaucgacgc ccugcgggac     1140

ggggagaagg acggccgcgu gcugguguac agcgaggccg ucaccgccgc cgggcucggc     1200

caccacuccg accggcgcac cggcgaggug uucgaggacg aggaggggcg gccccagcug     1260

ccguacuacg gcguggcccu ggagcgccac aucaugcccg ggaccggcga ccccagcgac     1320

uccgacgaga ugcgggucgg gcgccucacg aaccccaccg ugcacaucgg ccugaaccag     1380

cugcggcgca ucgugaaccg gcucauccgc gccuacggcc cgcccgcgga gaucgccauc     1440

gagcuggccc gggagcugaa gcugaccgac gaggacaaga agcgcaucaa caaggagaac     1500

aacagcaacc ggcucgacgc cgagcgccgc uccgagaagc ugcuggccau cgggaagccc     1560

gacaccggcg cgaaccgggc ccuccugaag cugugggagg agcucaaccc cgccaacguc     1620

cuggaccgcc ggugcaucua cagcggggug cagaucucca ucgacaugcu guucagcgcc     1680

gccguggagg ucgaccacau ccucccguuc acggcgaccc uggacgacuc caacggcaac     1740

aagaucgugu gcacccgcga ggccaaccgg gagaagcgca agcggagccc cuacgaggcc     1800

ugggggcaca gcgcccgcug ggaggagauc gccgcgcggg ccgcccagcu gccccgcaac     1860

aagcgguggc gcuucgagcc cgacgccaug gagaaguuca aggacgacgg cggcuuccag     1920

gcgcggcacc ucaucgacac ccaguaccug gcccgccugu cccgggagua ccucgccacg     1980

cuguacccgg acaaggggga gggcagcggg cacaucuggg ucucccccgg ccgccugacc     2040

gagauggugc ggcgcaagcu cggccugaac gaccugcucc ccgaccacaa cuucgggggc     2100

ggggccgacc aggccaagaa ccggcuggac caccgccacc acgcgaucga cgccgugguc     2160

guggccaucg uggaccgcgg caugcugaac cggaucgccc gcgccagcgg gcaggagggc     2220

gcggagggcc gggagcgcau caucaucccg gagcccuggg agggguuccg ggacgaccuc     2280

cgcaccgccg ucaacgccau caccgugucc caccggacgg accacggcac cgccagcaag     2340

gccgggcugc ccaagggcaa ggacgcgacc gccgggcgcc ugcacaacga caccgccuac     2400

ggcuucacgg gcgaguccga cgccaagggg aacagcaucg ugguccggcg cgugccccuc     2460

uccagcuuca agaagcuggc cgacaucgac cgggugcgcg acccggagcu gaaggcggcc     2520

cucuaccagc accuggacgg ccuggagggg aaggccuucg agaaggccau cggcgaguuc     2580

gggucccggg aguggcaccc cuacggcggc guccgccggg ugcgcgugac cgagccccuc     2640

agcgucaucc ccauccggga ccgcaacggg cacaucuaca agggcuacaa gggggacucc     2700

aacuaccggu acgacgugug ggagcugccg ggcgggaagu gggugaacga ggucgugagc     2760

auguucgacg cgcaccagcc cggcuggcag uccgccaucc gcgccgccaa ccccaccgcc     2820

cggaaggugc ugagccucca ccgcgacgac guccuggcga ucgagcgcaa cggcgccgag     2880

cgggagcugg ugcgcguggu caaguucucc gagaagcagu ucgccguggc gcccccgaac     2940

gagggcgggg cccucaaggc ccgggacgcc gacaaggggg accccuucaa guacagcuac     3000

cccuccccca acacccugaa ggcccugcgc gcccgccagg ugcggaucga cgagcucggc     3060

cggguccugg acccgggcuu ccccgcccgc acgaagcagc ggaagacccg caagcccaac     3120

uga                                                                   3123


<210>  1458
<211>  3132
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1458
augcagcgcg acaacccccu gguguaccgg cucgcccugg accugggcag caccucccuc       60

gggugggccg ucuuccgccu ggacgcggag ggcagcccgc uggccaucau caaggccggg      120

gcccggaucu ucuccgacgg ccgcaacccc aaggacggga gcucccucgc cgugacccgg      180

cgcgcggccc gggccaugcg ccggcgccgg gaccgccugc ugaagcggaa ggcccgcaug      240

cucggcgcgc uggagcggca cggcuucuuc ccccaggacg agagcgcccg caaggugcug      300

gagacgcuca accccuuccu gcugcgggcc caggggcucg aggucgcccu gaagccggag      360

gaguucggcc gcgcccuguu ccaccucaac cagcggcgcg gguuccaguc caaccggaag      420

accgaccgca aggacagcga cuccggcgcg cugaagcagg ccaucgggac ccugcgcgcc      480

cagaugcacg agagcggcug ccggaccgug ggcgaguggu ucuggaaggu gcgcauggag      540

cacaagcccg agggggucaa gggccagggg gugcgggccc gcuaccggga gacgcccuuc      600

accaccgacg agggcaagaa gcgcaucgac aagcgguacg accucuacgu ggaccgcgcc      660

auggucgcgc aggaguucga cgcccugugg gccgcccagg ccgcguucaa ccccgcccug      720

uucaacgagg ccgcccgggc cgagcuccgc gacauccugc ugcaccagcg gaagcuccgc      780

ccggugcagc ccggccggug cacccuggug cccgaggagc cccgcgcgcu gcucgcccug      840

uccagcacgc agcgguuccg caucuaccag gaggucaacc accuccgggu gcugcacgac      900

gagcugcgcg aggagacccu gacccucgcc cagcgggacc agaucguggc ccugcuggag      960

aagcaggcga aggucaccuu cacggccaug cgcaaggccc ucggguucuc cgcccuguuc     1020

aaccuggagg acgccaagcg cgacggccuc aaggggaacg cgaccgccgc cgcccugagc     1080

aagaaggacc uguucggccc gcucugggcc ggguucggca ccgcgcugca ggacgagauc     1140

gugacccagc ugaugacgga ggaguccgag ccccggcuca ucgccuggcu gcaggagcac     1200

accggcgugg acgaggcccg cgcccaggcc aucgcgaaca ccgggcugcc cgagggcuac     1260

gggagccugg gccggaccgc ccucgcccgc aucguccccg agcugcagcg ggacgugauc     1320

acguacgaca aggccgugca ggcggccggg uucgcccacc acuccgaccu gggcuucagc     1380

uucgagcacg gcgccgacga ggucgagcgc aucggggagc ggaccaucgc cuccaccggc     1440

gagaucaagc cgguguucgc guucaagcag cuccccuacu acgggcgcgc ccugcagcgg     1500

cacguggccu ucgccaagga caacccccgc aacgacgagg agcgguacgg caagaucgcc     1560

aaccccaccg uccacaucgg gcugaaccag gugcgcgucg uggugaacgc gcucaugcgg     1620

cgcuacggcc ggccggccga ggucgugauc gagcuggccc gcgaccugaa gcagagccgg     1680

gagcagaagc ucgaggccca gcagcgccag gccgacaacc agaagcggaa cgcgcgcauc     1740

cggcgcagca ucgccgaggu gcugggcauc uccgaggagc gcguccggac gcuggacauc     1800

cagaagugga uccucuggga ggagcugagc cacgacgccg ccgaccgccg gugccccuac     1860

uccggggugc agaucagcgc guccaugcug cucggcgagc gcguggagau cgagcacauc     1920

cugcccuuca gcaugacccu ggacgacucc cucaacaacc ggaccgucgc ccugcgcgag     1980

gccaaccgga ucaagcgcaa ccgggccccc ugggaggccc gcgcggacuu cgaggcccag     2040

ggguggagcu acgacggcau ccugcagcgg gccgagcgca ugccgcuccg gaagcgcuac     2100

cgguucgccc ccgacggcua cgagcgcugg cugggggagg acaaggacuu ccuggcccgg     2160

gcgcucaacg acacccgcua ccugucccgg guggccgccg aguaccugcg ccucgugugc     2220

cccggcagcg ccacgcgcgu cauccccggg cagaugaccg cccugcugcg gggcaaguuc     2280

gggcucaacg acgugcuggg ccuggacggc gagaagaacc gcaacgacca ccggcaccac     2340

gcgguggacg ccugcgucau cggggugacc gaccagcgca ugaugcagcg guucgcccag     2400

gccaacgccc aggcgcgcga ggacggccuc acccggcugg uggaggacau gccgcugccc     2460

uggcccacgu accgcgacca cgucgagcgg gccgugcacc acaucugggu gucccacaag     2520

ccggaccacg gguucgaggg cgccaugaug gaggagacca gcuacgggau ccuccgcgac     2580

ggcuccauca agcagcggcg caaggccgac ggcagcgcgg ggcgggagau cuccaaccug     2640

auccgcaucg ccgagcccgg ccagccccag cggcacgggg ucgacgccga gggccgcccc     2700

cugccguaca agggguacgu gggcggcagc aacuacugca ucgagaucac cgugaacgac     2760

aaggggaagu gggagggcca ggucaucucc accuucgacg ccuaccggau cgugcgcgag     2820

ggcgggcucc ggcagcugcg ccaccccacg caggggcaga acggccgggc ccuggcgaug     2880

cgccucguga ucggcgacag cguccgccug gaggccgggg agggcucccu gcagacccuc     2940

cggguggcca agaugagcgc cgccggccag guguucauga gcgaggucaa ccaggcgaac     3000

guggacgccc gcaaccggaa cggggaccug cccuacgugu ccaagauggc cggcagcuuc     3060

cagaaggccc gcgcccggca ggucaccauc ucccccaucg gggagcugcg cgacccgggc     3120

uucaaggggu ga                                                         3132


<210>  1459
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1459
augaaguaca cccugggccu cgaccugggg uacaacagca ucggcugggc cauccuggag       60

aacgacgaga acgggucccc caucaagcug uucaacgccg gcgugcacau cuucgagccg      120

agcaucgagg acaucgagau cgacgggaag ggcaagacca agaacgucaa gcgccgggag      180

gcgcgccugc ggcgccggca gcuguuccgc aagacgaagc ggcucgagaa gcugcugaac      240

uucuucaucc aggagggcuu cuuccagaac cagaaggugc ucaucaacga ccccuucgag      300

cgcgccgagu uccuguacca gcuggacaag aagucccgga accccuacau ccuccgccgg      360

gaggcccugg acaaccgccu gagccucgag gagaucgccc ggaucuucua ccacaucgcc      420

cagcaccgcg gguucaaguc caccaagcgg gagcuggcgg agaugaacaa ggacgagaag      480

aagaagaagg aggacggcau cauccagagc gagaucaaca cccugaagga ggagaucaag      540

aagaacuccu gccgcacccu cggggaguac uucgccaagc uguucgagaa cggcgagaag      600

auccgggggc gccacacgga ccggaagaug uacgaggagg aguuccagaa gaucuucaac      660

aagcagaagg aguucuaccc caacaucuuc accgacgagu ucaagaagaa gcuguacaag      720

cucaucuucc accagcgcga gcucaagagc cagaaggaca aggugggcuu cugcucccug      780

gagcggggca agaagcgcgc cccgcucgcc cugcugguca gccagcgcua ccgguacaug      840

ugcaagguga acaaccucaa cgugaucgac accgagaccg gggagaaccg cgagcugacg      900

gucgacgaga agaagaagcu guacgagaag cucgagaacu ccgagaagcu gagcuucaag      960

gagaucaaga agauccugaa ccucuccgag aagcgguaca ucuucaaccu ggagaagucc     1020

gagaaguaca ucaucggcaa caagaccaac gugaagauga agaacaucau caaggacugg     1080

gacaacuucg acgaggacaa gaagaacuuc uucaucaacg aggcgcgcac cguggucaag     1140

aucgagaccc uggagcggcg cgggaagaag cucggccuga gcgacgagga gagcaaggag     1200

uucuccaagg ugguguucga ggacggcuac cugaacuuca gcaagaaggc cgucgagaag     1260

cuccugcccu accugaagga ggggaagaag gaguacgagg ccaucaagcu cgccuacccc     1320

gagaacuccg ugcagaacga caaccugaag gagcuggacu uccugccccc gaucaagagc     1380

aaggagcuca agaaguacuu cgaggugcgg aaccccaucg ucgagaaggc ccugacggag     1440

acccgcuacc ucgugaacuu ccugaucaag aaguacggca agcccgagac caucaagauc     1500

gagcuggcgc gggagcugaa gcucagcaac aagcagaagg agcgcaagca caaggagaac     1560

caggagaacc ggaagaagcg cgaggaggug aucgagaagc ugaagaacga ccugaagauc     1620

uccgagccca ccgagacgca gauccagaag uaccuccugg ccgaggagug cgacugcaag     1680

ugcccguaca ccgggcggag caucuccaug gacagccugu ucggcgagca cccccucuuc     1740

gacaucgagc acaucauccc cuucgaccgc ucccuggaca acagcuuccu gaacaagacc     1800

cucugcuacu ccgaggagaa ccggaagguc aagcacaaca agacccccua cgaggccuac     1860

uacgggacgg agaaguggga ggccaucauc gagcgcguga agaaguucaa gagcaacgug     1920

cggaacgaga agcugcgccg guuccagaug accaaggagg acgucgagaa cuacauccag     1980

aacuucucca cccagaaccu gaacgacacc aaguacgcca gcaagcucgc gaagaaguac     2040

cugggccugc ucuacggcuc caucaacgag gacgggaucg acaagaaccg caagcgccgg     2100

aucuucgcca gcuccggcaa ggugacgagc acccugcgcu acgcccuggg gcucaacacc     2160

auccugaacg acgagaacau caagacccgg gaggaccacc gccaccacgc caucgacgcc     2220

aucacgaucg cgcugaccga cuccagcaug aucaagaaga ucuccgacgc cagcaagcgg     2280

gagaagaaca ucaacucccu cuucaaggac uuccaggccc cgugggagaa cuuccgcaag     2340

gagguggagg agaucaucgc caagaugaac aucagcaucc ggaucaacaa gaaggucagc     2400

ggccagaucc acgacgagac cuucuacaag aagcccuuca aggaggagga gaaguccuac     2460

acccaccagc gcaucaaccu ggaggagcug acgaucaagg acgaggacaa gaagaagcag     2520

agcuccaagc ucaucgacaa caucguggac agcaagaucc ggaacaucau caucaacucc     2580

cugaacgggg agaagcccaa gauccgcuuc aaggacccca aggaccaccc guacaucauc     2640

cagaagaacg gcaagaagau ccagauccac aaggucacca ucaagaccaa gcuggagacc     2700

uacaacgaga ucggccccga gaaggccaag cgguacguca agagcuccga cagccaccac     2760

aucgagaucu ucgaggaccu caagacgaag aagugggacg gguacguggu gucccugcug     2820

gaggcguacg agcgcaagaa gaagaagcuc cccaucguga acaagaaccu gggcgagggg     2880

aagaaguucc uguucagccu cgccaagggc gagaucaucg agcuggacac cgagaacggg     2940

aagcgggagc uguucguggu ccgcaucauc uccgccagca acaaccagau caaguucacc     3000

cggaucaacg acucccgcaa gaucaccgac auccccgcca ccggccucac ggcgugcccg     3060

agcucccuga agaagcggaa cugcaagaag guggugauca acauccuggg cgagaagcac     3120

uacagcaacg acuga                                                      3135


<210>  1460
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1460
auggccgaga agaaguucaa guaccgccug ggccucgacc uggggaccaa cagcaucggc       60

uggugcaugc ugcggcucaa caaggacaac gagcccgccg cggugaucaa guccgggguc      120

cgcaucuucc acgacggccg gaacgagaag accaaggagc cgcuggccgu ggugcgccgg      180

aacgcccgca gcauccggcg caaccuggac cggaagaucu cccgccggaa ccaccuccug      240

aacacgcugg ucaaguacuu ccuccugccc agcaacgaga ucgagcgcaa ggcccuggcc      300

gugcucaacc ccuacgagcu gcggucccgc gcggugaacg agcggcugga gcuguucgag      360

cucgggcgcc ugcucaugca ccugagcaag cggcgcggcu ucaaguccaa ccggaagguc      420

gacaaggagg acagcuccgg caagaucaag cccgccaucg agcgccugaa gaacaagaug      480

caggagacca acaagaagac cgccggggag uaccucuaca gccugaugca gaacggcaag      540

ccggugcggg cccgccuggg gcgcaucgac ggcuccaacg gguacgagau cuaccucgac      600

cgggcgcuga ucgaggagga guacaaccug aucaugaacg agcagaagaa gcaccacaag      660

gagcucaccg acgacaucau caacgagauc uucaacauca ucuucuacca gcgcccccug      720

aagaagcaga ucaucggcaa gugcagccug acgggcgagg acaccaagcu caagaaggcc      780

cacgugcugg cccaggacuu cauccugcuc cagaaggucc aggaccugcg gguguucgac      840

gacaaccagc uggaguuccg cccccucucc aacgacgaga aggacaucau caagcgggag      900

cugcgcaaga agaaggaggu gagcuacgag aacauccgga agcugcucaa gaaguacuac      960

gagaacguca aguacgggau guucucccac gagacccugu acaagaagcu gcagggcaac     1020

aagaccaacg cccucaugag cgacaaggag aucaucgggg acuccuggua cagccugucc     1080

cccgaggugc aguacgacau caucgaggag cuguacagcg acaaggacaa cgacgagcuc     1140

agcaagcugu acuccgagaa guucaaccug aacgagagcc agaucaacgg ccuccugaac     1200

aaggccaucu accgccugga cuccggcuac auccgguacg ggaagacggc ggugaccaac     1260

cugaacagca ucauggacaa ggacaaccuc aaccugcacg acgcccgcga ggaggccggc     1320

uacgagaagg agaacaagca ggacgccucc gaguaccugg aguacuacgg gaagguccuc     1380

ccggacagcg ugguggaccc ccagaucgag aaccccaaga acgacgagga gcgguacggc     1440

aagaucgcca accccaccgu ccacgcggcc cugaaccagc ugcgcaagcu cgugaacgag     1500

cugaucaagc uguacgggaa gccggagcag aucgugaucg agcucgcccg ggaccugaag     1560

aacucccgca aggccaagga cgucaucaac aagaagaucg ccgagaaccg gaagcugaac     1620

gaggaggugg acaagacccu ccaggaguac agcgagaagu acaacacgca caucgugaag     1680

aacaagcuga accgcgacaa ggucaagcug uggcgggagc ucggcaagga cccccuggag     1740

cgcaagugca ucuacaccgg cgagcagauc aacaucgcga agcuguucuc cgacgaggug     1800

cagaucgagc acaucguccc cuacagcaag acccucgacg acuccuucaa caacaagacc     1860

cugagcauga agcaggccaa cuacuacaag gggaacaaga cgcccuacga ggccuucaag     1920

gacuccaagg acggcuacaa cuacgacgac aucaucgccc gggccaagcu gcucaacaac     1980

gacaacaagu ucaagcgcuu caccaaggac gcgauggaca ucuacaaccg cgacgggaag     2040

gacuucaucg cccggcagcu gagcgacacc caguaccugu ccacccgcgc ccucaucuac     2100

cugcagagcc uguacgagaa ggcccaccac uccagcaucu ggacgauccc gggccagcuc     2160

accgcgcuga uccggaacua ccuggggcuc aacacccuga ucuccaacga caagaacaag     2220

aaccgcaacg accaccggca ccacgccgug gacgccuucg uggucgccgu gaccagccgc     2280

agccugcucc agaagaucuc cuacgccgcg agccugcagg aggaccugga caaccggaac     2340

guguccagcu accgcaacaa gcuccuggag aagaugcccg agcccuuccc caacuaccgg     2400

aagucccugg agcucgccau cgacaacauc aagacgagcc acaaggccga ccgcuccauc     2460

agcggcaagc ugcacgagga caccgccuac ggcaucgucg agaacgacga caaguacaac     2520

guggugaccc gguggaccgu cgacaaguuc aaggagcaga agcacuucga gacgauccgc     2580

gacaacgagc ugaaggagau cgccugcucc gacaaggagc uguucgcgaa gaucguggag     2640

acccggaaga ucaagagcau ccgcaagcuc gccaaggaga acccggugau caagaucaag     2700

gacaaguccg ggcgggacua caaggccuuc gccggcggga acaacaucug cgucgagauc     2760

uacgaggugg acggcgugcg caagagcgag gucauccagc uguucgacgc cgcgcagaag     2820

ggguucaugc ccgaguggau gaagaaguac cccaacggca agcuccugau gcggcuguuc     2880

aagggcgacg ugaucggguu caucgagaac cgcaaguaca acuacuacac caucaagggc     2940

aucuccaucg ccgaccagaa ccucaagcug cuggccauca acaaggccga ggggaucuuc     3000

aagaagggcc ucaacauccu guucgacagc aacaacaaga acucccugaa cgcgaagcag     3060

uucaacauca gcgugaccgg caucgucacg aagaagaaga ccgccgacgu gaacuucugg     3120

aagaaccgga aguga                                                      3135


<210>  1461
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1461
augcugcaca agaugcgcua ccggcucgcc cuggaccugg gcagcaccuc ccucgggugg       60

gccauggugc gccugaacgc gaaccagcag cccugcgccg ucaucaaggc cggcgugcgg      120

aucuucagca acgggcgcaa cccgaaggac ggcuccagcc uggccgugac ccggcgcgag      180

gcccggucca ugcgccggcg ccgggaccgc cuccugaagc ggaaggcgcg caugaugcgg      240

acgcugaucg aguacggguu cuuccccgcc aacgagaccc agcgcaaggc ccucgagaac      300

cugaaccccu acgcccugcg ggcgaagggc cucgacgagg cccugacccc cagcgaguuc      360

gcccgcgccc uguuccacau caaccagcgg cgcggcuuca aguccaaccg gaagaccgac      420

aagaaggaca gcgacuccgg ggcccucaag acggcgauca agcagcugca cagcguccug      480

gacccgcagg gcaacgacgg gaagccccgc accgugggcg agcuccugua caagcgcuuc      540

accgaccugu ccaagcuccc caaggaccgg accgugcgcg cccgguaccg ccaggacaag      600

aucgucaagg acgacgggaa gacgaagauc gacaaguacu acgaccugua caucgaccgg      660

gccaugaucg agcaggaguu cgacgcccug uggaagaagc agagcgagcu caaccccauc      720

cuguucaccg agaacgcccg cgcggaccug aaggacgugc uccuguacca gcggucccug      780

aagccgguga agcccggccg cugcaccuuc augagcgagg aggagcgggc gccccucgcc      840

cugcccucca cccagcgcuu ccggauguac caggagguca acaaccugcg cauccuccgg      900

gagggccuga aggaggagag ccugacgcuc cagcagcgcg acgaccugau caacgcccug      960

gagaagaaca accggcgcac cuucacccag aucaagaagc uccugggggu gggcgggucc     1020

gugcaguuca acuucgagga cccgaagcgg gaggagcuga agggcaacac cacgagcgcc     1080

auccucggca aguccgagca cuucggggag gccugguucg ccuucaacga ggccaagcag     1140

gacgccaucg uccugcagcu gaucaaggag gagaacgagg ccaagcucgu gcgcuggcug     1200

caggacgaga ccggcaucga cgagaagcgg gcggagguga ucgccaacgc cgggcugccc     1260

gagggcuacg ggagccucug caucgaggcc cuggcgcgca uccugcccga gcugcgccgg     1320

gacgucguga ccuacgacaa ggccgugcag gccgccggcu ucgagcacca cagcaagcuc     1380

aaccgcaacg aggagauccc cgacaucacc uucaagaucg aguccaucga ccggaacagc     1440

ggcgagauca agcaguucca ccuccacaag gagcugccgu acuacgggga guaccugcag     1500

cgccacgucg gcuucggguc cggcaagccc gaggacagca ucgagaagcg guacgggaag     1560

aucgccaacc ccacggugca caucggccug aaccaggugc gcgucguggu gaacgcgcug     1620

aucaagcggu acggccaccc cuccgagauc aucgucgagg uggcccgcga ccucaagcag     1680

agcaaggacc ggcgcgacga ggagaacaag cggcaggccg agaaccagaa gcgcaacgag     1740

cggcugcgca aggacaucgc cgacauccug gggaucuccg aggagcgggu ccgccgggac     1800

gacaucgaga agaugauccu cugggaggag cugagcuucg acccggccga ccgccggugc     1860

cccuacuccg gcguccaggu gagcaccgug augcugcucu ccgacgaggu ggagguggag     1920

cacauccugc ccuucagcca gacccuggac gacucccuca acaacaagac cgucgcgcug     1980

cgccaggcca accggaucaa ggggaaccgc acgcccuggg aggccuucgg caccagcaac     2040

aucuccgggu ucgacuacag cggcauccug acccgcgccg agcucaugcc gaaggccaag     2100

cgguaccgcu ucgcggagga cggcuaccag cgguggcuga aggacgacgc cggguuccug     2160

gcccgcgccc ucaacgacac ccggcaccug uccaagaucg cgcgcgagua caugagccug     2220

aucugcccca acacgcgggu gauccccggc cagaugaccg ccaugcuccg ccggaacuuc     2280

gggcugaacg acgugcuggg ccucaacggc gagaagaacc gcaacgacca ccggcaccac     2340

gccgucgacg ccugcgugau cgccgugacc gaccaggggc ugcugcagcg cuucgcggcc     2400

gccuccgcca gcgcccggga gcgccagcuc aaccggcugg ucgagaacau gccccugccg     2460

ugggagagcu accgcgagca cgugcggcgc gcgaucgacg gcaucugggu gucccaccgg     2520

cccgaccaca gccacgaggg ggccaugcac aacgacaccg ccuacggccu ccgcgggaac     2580

ggccgcgucu ccuuccacaa gaugguggag ggcaagcggg aguacaucga ggacaaccug     2640

aaggugaucg agaucacgga caccaaggcc aucgaccgcc acgggcugcu ccccaccggc     2700

aagccgcggc ccuacaaggg guacaagggc gacagcaacu acugcaucga gaucguccgc     2760

aacgagaagg ggcgguggga gggcgagauc aucuccaccu ucgacgccua ccagcuggug     2820

cgcaaguacg gcaucgugca gcugcgguac cccguccuca gcguguccgg gaagccccug     2880

gugaugcgcc ugauccggaa cgacuucguc cgccucgagc acgagggcau cacgcggacc     2940

cugcgcgugu gcaagaugcg ggaggacggg caggucgcgc uggccgacgu gaccgaggcc     3000

aacguggacg cccgcacccg gaccaaggag aucugcuacg uguucaagac gggcgggagc     3060

cuccagaagg cgaaggcccg ccggaucacc aucuccccga ucggcgagcu gcgcgacccc     3120

ggcuucaagg aguga                                                      3135


<210>  1462
<211>  3138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1462
augcagaagg agaagccccu ggccuaccgc cucgcccugg accugggcag caccucccuc       60

gggugggcgg ugcugcggcu gagcgccccg ggcgagcccu ccgccgucau ccgcgccggg      120

agccggaucu ucuccgacgg ccgcaacccc aaggacggga gcucccucgc cgugacccgg      180

cgcgcggccc gggccaugcg ccggcgccgg gaccgccugc ugaagcggaa ggcccgcaug      240

cucgcggccc uggagcggca cggcuucuuc ccccaggacg aggccgcccg caaggcccug      300

gagaagcuca acccguacgg ccugcgggcg cgcgggcugg agagcgcccu caagcccgag      360

gaguucggcc gggcccuguu ccaccugaac cagcgccggg gguuccaguc caaccgcaag      420

acggaccgca aggacagcga cuccggcgcc cucaagcagg ccaucgggac ccugcgggcg      480

cagaugcacg agagcggcug ccgcaccgug ggcgaguggu ucuggaaggu ccggauggag      540

cgcaagcccg agggggugaa gggccagggg gugcgggccc gcuaccggga gacccccuuc      600

acgaccgacg agggcaagaa gcgcaucgac aagcgguacg accuguacgu cgaccgcgcc      660

augguggccc aggaguucga cgcccucugg gcggcccagg ccaccuucgc cccggcccug      720

uucaacgagg cggcccgggc cgagcugcgc gacauccucc ugcaccagcg gaagcugcgc      780

cccgugcagc ccggccggug cacccucguc cccgaggagc cgcgcgcccu gcuggcgcuc      840

cccuccacgc agcgguuccg caucuaccag gaggugaacc accuccgggu gcugcacgac      900

gagcugcgcg aggaggcccu gacccuggcc cagcgcgacc agaucgucgc ccuccuggag      960

cggcaggcca aggugaccuu caccgcgaug cgcaaggccc ugggguucag cgcccucuuc     1020

aaccuggagg acgccaagcg ggacggccug aaggggaacg ccacgaccgc ggcccucggc     1080

aagaaggacc uguucgggcc ccugugggcc ggcuucgaca ccgcccucca ggacgagauc     1140

gugacccagc ugaugacgga ggaguccgag ccccgccuga ucgccuggcu ccaggagcac     1200

accggcgucg acgaggcgcg ggcccaggcc aucgccaacg cggggcugcc ggagggcuac     1260

gggagccugg gccgcaccgc ccuggcccgg aucgugcccg agcuccagcg cgacgugauc     1320

accuacgaca aggccgucca ggccgcgggg uucgcccacc acuccgaccu gggcuucagc     1380

uucgagcacg acgccgacga gguggagcgg aucggcgagc gcacgaucgc cuccaccggg     1440

gagaucaagc ccguguucgc cuucaagcag cugcccuacu acggccgggc gcuccagcgc     1500

cacgucgccu ucgccaagga gaacccgcgg aacgacgagg agcgcuacgg gaagaucgcc     1560

aaccccaccg ugcacaucgg ccugaaccag gugcgggucg uggucaacgc ccugaugugc     1620

cgcuacgggc ggcccaccga gguggugauc gagcucgcgc gcgaccugaa gcagagccgg     1680

gagcagaagc uggaggccca gcagcgccag gccgacaacc agaagcggaa cgcccgcauc     1740

cgccggagca ucgcggaggu ccucggcauc uccgaggagc gcgugcggac gcuggacauc     1800

cagaagugga uccuguggga ggagcucagc cacgacgccg ccgaccgccg gugccccuac     1860

uccggcgugc agaucagcgc cuccaugcug cugggggagc gcgucgagau cgagcacauc     1920

cucccguuca gcaugacccu ggacgacucc cugaacaacc ggaccguggc ccuccgcgag     1980

gcgaaccgga ucaagcgcaa ccggaccccc ugggacgccc gcacggacuu cgaggcccac     2040

ggcuggagcu acgacgggau ccugcagcgg gccgagcgca ugccccagcg gaagcgccac     2100

cgguucgccc ccgacggcua cgagcgcugg cugggcgagg acaaggacuu ccucgcgcgg     2160

gcccugaacg acacccgcua ccugucccgc guggccgccg aguaccuccg gcuggucugc     2220

ccggggagcg ccacccgcgu gauccccggc cagaugaccg cgcugcuccg ggggaaguuc     2280

ggccugaacg acgugcuggg gcucgacggc gagaagaacc gcaacgacca ccggcaccac     2340

gccgucgacg ccugcgugau cggcgugacg gaccagcgca ugaugcagcg guucgcccag     2400

gccaacgcgc aggcccgcga ggacgggcug acccggcugg ucgaggacau gccccucccg     2460

uggcccaccu accgcgccca cguggagcgg gccgugcgcc acaucugggu cucccacaag     2520

cccgaccacg gcuucgaggg ggcgaugaug gaggagacca gcuacggcau ccugcgggac     2580

gggacgauca agcagcgccg gaaggccgac ggcuccgccg gccgcgagau cagccggcug     2640

auccgcauca ccgagcccuc ccagccgcag cggcacgggg uggacgccga gggccgcccc     2700

cuccccuaca agggguacgu gggcgggagc aacuacugcc uggagaucac cgucaacgac     2760

aagggcaagu gggagggcca ggugaucucc accuucgacg ccuaccggau cgugcgcgag     2820

ggcgggcugc gccagcuccg gcacagcacc caggggcaga acggccaggc gcuggccaug     2880

cgccugguca ucggggacuc cgugcggcuc gagguggacg agcaggagcg cgucaugcgg     2940

aucgugaaga ucaacggcaa caacggccag guguucaugg cccccgucca cgaggccaac     3000

guggacgccc gcaacagcga caagaacgag gcguucgccu acacgagcaa gaucgccggg     3060

uccuuccaga agaccaaggc ccgccaggug accaucagcc cgaucggcga gcugcgggac     3120

cccggguuca agggcuga                                                   3138


<210>  1463
<211>  3138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1463
augaagcccc gcaaccugaa cuacauccuc ggccuggacc uggggaucgc cagcgugggc       60

ugguccgucg uggagaucga cgagaacgag uacccgaucc ggcucaucga cguggggguc      120

cgcaccuucg agcgggccga ggugcccaag accggcgaga gccuggcgcu ggcccgccgg      180

cucgcccgcu ccacgcggcg ccugauccgg cgccgggccu uccgccugcu caaggccaag      240

cggcugcuga agcaccacca gaucgugaac gcggaggagc ucacccagcu gcccaaccag      300

ugcugggagc ugcgcgucaa ggggcucgac agccugcugu ccaacaccga gugggccgcc      360

gugcuccugc accuccucaa gcaccggggc uaccugagcc agcgcaagaa cgaggcccag      420

aacgcggaca aggagcuggg caagcuccgg gaggggaugg acaacaacuc caagcugcug      480

cucgagaaca acuaccgcac ccccgccgac aucgccguga agaaguucgc cgucgaggag      540

ggccacaugc ggaaccagcg cggggccuac acgcacaccu ucaaccggcu ggacauccug      600

gcggagaugc agcuccuguu caagacccag cgcgagcugg gcagcaccua cgccaacggg      660

gagcucgagc aggccuucug cgagcugcug cucuggcaga agcaggcccu gaaccgcucc      720

cagaugcuga gccucguggg caagugcacg uucgagaagg aggagaagcg ggccgcgaag      780

gccuccuaca gcgccgagcg cuucguggcc auccagaagc ugcagaaccu gcggauccuc      840

gagaacggcg aggagcgcgg gcuguucgac uccgaguuca gccugcuccu ggagaacgcc      900

uacaaccuga aguccggccu caccuacaag cagguccgga agauccugag ccuguccgag      960

aacgcgaucu ucaaggggcu gccguaccuc agcgacgacc uggagaagcc cgagaagacc     1020

caguuccugg ccuucaaguu cuaccaccag cucgccgaca uccugaagaa caacggcuuc     1080

uccgacgagu ggcagaagcu gagccaggag cccacccucc uggacaagcu gggcacggag     1140

cucagccugu gcaaggagga gaacgaguuc aucgcccagu ucaacgggga gcugcccgag     1200

gccaugcucu ccacccuguu caaccacacc aacuucgaca aguucaucca caucagccug     1260

aaggcgcuca acaacauccu gccgcugaug gagcagggca acgacuacac caaggccugg     1320

cgcaaggugu accccgagcc cacgaagaag gacgagaaga cccugccccc gauccccgcc     1380

gacgagaucc ggaaccccgu gguccugcgc ucccugagcc aggcccggaa ggugaucaac     1440

gcggugaucc gccucuacgg gucccccgcc cggauccaca ucgagaccgc ccgcgagcug     1500

ggcaagagcu acgacgaccg gcagaagauc aagaagcagc aggacaagaa cuccgacgag     1560

cgcgaccagg ccgucaagaa guuccuggag gagugcccga acuucgccaa caaggugaag     1620

gggaaggaca uccucaagau ccggcuguac aucaaccagg acggcaagug ccuguacagc     1680

ggcaagcccc ucgaccccca ccgccugcug gagaucgggu acguggagau cgaccacgcg     1740

cuccccuucu cccggaccug ggacgacagc cagaacaaca agguccuggu gcuggccaac     1800

gagaaccaga acaagggcaa ccagacgccg uucgaguggc ucgggaagga cgagcaccag     1860

ugggcccugu ucguggcccg cgucaacggc ugccgguucc ccuacgccaa gaagcagcgc     1920

auccugacca agaagcucga cgagcagggg uuccugaagc gcaaccugaa cgacacccgg     1980

uacguguccg cguaccucau gaagcacauc aaggagaacc ugcaccuggu cggcaagggc     2040

agcgacaagg uguucgccuc caacggccag gugaccaacu uccuccgccg gugcuggggg     2100

cuggagaaga agcgcgagga gggggaccgg caccacgccc uggacgccau cgucguggcc     2160

ugcagcacgg cguccaugcg ccagaagauc acccucuuca agaaguacca gcgguggaac     2220

cugaagaccg gcaagcacau cgaccaggag accggggaga ucaucccccu gcacuucccc     2280

gccccguggg acuucuuccg ccaggaggug augauccgga ucuucagcga gaugccccag     2340

gaggaccuca ucaugcagcu gcccgaccgc ccccaggcca accacgaguu cguccagccg     2400

cuguucgugu cccgggcccc cagccgcaag auguccggcc agggccacga ggcgaagcuc     2460

cggagcgccc gcaugcugga ggccacgggc aagagcauca agaaggaguu ccugaccgac     2520

cucaccauca agaaccugga gcacauggug aacaaggagc gggagcccga gcuguaccag     2580

gcccucgucg agcacuucaa gaaguacggg gacaagccga aggagaaguu cuucaagaag     2640

ggcggggugg aggugaaguc cauccgcauc agcaagaccc agaacaaguc cgucaaccug     2700

ggcaacaaga cgaucgccga caacggggac aucgugcgga ccgaccuguu ccucaagaac     2760

aagaaguacu acuucgugaa caucuacgcg uggcagguca gcaagggcau ccugcccaag     2820

gagaccacca cggggaacau ccuggacaac uccuacgagu uccaguucag ccucuucaag     2880

aacgaccugc uggagauccc ccaccccaag aacgagaacg acuccauccu cgccuacuuc     2940

auccgcccgg acgacgagcg gcgcuggauc cugaaguucc acgacaacgc caagaucccc     3000

gacaucuacg gcaagaagga cgagaccagc auccggcugu ccauccaggg gcagaaguuc     3060

aucaagaagu accaggugga cgagcucggc aagaacaucc gccccugccg ccccaccaag     3120

cggcagggcg ugcgcuga                                                   3138


<210>  1464
<211>  3141
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1464
augaagaucu acagcuucga caucggcgug gccuccaucg ggugggccuu caucgagaac       60

gagguccugc aggacugcgg cauccgcauc uucaccaagg cggagaaccc caagaacggg      120

gccagccucg cccugccgcg gcgcgaggcc cggggcgugc gcaagcggcu ggcccgccgg      180

cgcgggcggc ucaacaccau caagcagcug cugugcaagg aguucggccu ccagcuggag      240

gacuaccugu ccaacgacgg cgagcucccc aaggcguaca cggccagcaa ggccaacccc      300

cugaaguccc ccuacgagcu gcgcacccuc gcccugagcc agaagcugga cuccaaggac      360

cucgugcggg ucauccugca caucgcgaag caccgcgggu acggcaacaa gcacgccaag      420

gacagcaagg acaccgaguc cgggaaggug aagaaggcca ucgaguucaa ccggaagacc      480

cuggccgaga agggcuaccg cagcgugggg gaguaccucu accgggaguu cuuccagcag      540

ucccgcauca gccagaacaa cggcgccacg gaguucguca acgugcggaa caagaccggc      600

aacuacgagc acugcguggc gcaggacaug cugaaggacg agcuggagcu caucuuccgc      660

aagcagaagg agcuggggcu gcaccucucc gacggcuucg agaagcagcu gcuggacaag      720

aucuucgagc agcggccgcu caagagcuuc gccgacaagg ucgggaagug ccaguucauc      780

gccggcgcca agcgcgcccc caaggacucc aucagcgcga ucgaguucgu ggcccugucc      840

cggaucauca acacccuggc caaccucagc aagaaguccg gcgagaugua cgacaaggcc      900

auggugcuga ccauccugga cagcguccuc gagaaggggg agaugagcua cuacgcccug      960

cgcgaggcga ucaaccugga cgagaagauc cgcuucgccg acucccggcu cgacuacagc     1020

aaggagauca aggagacgga gaaggugaag uucguggagu gcaagaaccu gaaguccuuc     1080

aagaaggucc ugggcaacug cuacaagacc cucgagcgca agcacaucga cagcaucgcc     1140

gagcggaucg ccgugaucaa ggacguggag aagcugcaca aggagcugga guccuacugc     1200

gccaccgagc agcucagccu gaccuccgag cagauccagg cgcugagcca ccucaacuuc     1260

uccagccaca ucucccugag ccugaaggcc cucgagcaga uccugcccuu caugcgcggg     1320

gagcgggagg cccgcuccag cgacgccgac uacugcaucg gcaucgacga guccggggag     1380

agccagugcc ugcgguacga cgaguccguc gagaaggcgg gccugaaggc ccucagcaag     1440

gacacgcuga agggccagau ccugcccccg cucaacgagu ucgagcccua ccuggccaac     1500

cccguggugg cccgcgcccu ggcggaguac cggaaggucc ucaacgcccu gcugaagcag     1560

uacgggagcc cccacaagau ccacaucgag uacgcccgcg aggccaagcu caacaccacc     1620

gagcggcaga aguacgagaa ggagcagaag gacaacuacg ccgcgaacca ggccgcccac     1680

aagaagugcc aggagcuggg ccuggagccg agcagcacca accuccugaa gcugaagcuc     1740

uggcaggagc agggcgggcu gugccuguac uccggggcca agaucacgau cagcuaccuc     1800

caggacccca ccgcccucca gguggaccac aucuaccccu acucccgcag cuucgacgac     1860

uccuacauga acaagugccu ggugcucacc aaggcgaacc aggagaaggg caaccggacc     1920

cccuucgagg ccuucggcac ggacagcgcc aaguggggcg ucaucaccac ccuggcccag     1980

aagcugccgc acaagaagcg ccggcgcauc cucaacaccg cguucaacga caaggaggcc     2040

ggguucaagc ugcggaaccu gaacgacacg uccuacaucg cccgccucgu ggccgacuac     2100

accgagaccu accuggaguu ccugagccuc gucgaguccg agaacaccac gcuggggaag     2160

ggccagaagg ggagcaagaa gcacaucgcc aucgugaacg gcgcgcugac cuccaccaug     2220

cgguacuacc ucggcuucgc cagcaaggac cgcgacaacc accuccacca cgcccuggac     2280

gccgugauca ucggguucau gaacgacucc gucaucaagg ccuucagcga cuucaagaag     2340

acccaggaga cguccaaggc ggccuacuac gcccacaccc ugagcaagca ggaguacaag     2400

aagcagcgcg ccuucaucca gcugcccucc ggcgagaacu uccgggccaa cgugcuggag     2460

aaggcggaga gcguguucgu cagcaagccg ccccgcaagc gggugcgcgg ggcccuccac     2520

gaggaguccu ucuacagcca gaacgacacc aagcugcuga aguccuacgg cggcgccgag     2580

gggaucgaga aggcccucca gcugggcaag auccggcaga ucgggaccaa gaucgugagc     2640

aacggcgcga ugguccgcgu ggacaucuuc aagcaccgga aguccgggaa guucuacggc     2700

gugcccgucu acacgaugga cuucgcccug ggcauccucc ccaacaaggc cguggugcag     2760

gggaaggaca agaacgacgu caucaaggac uggcuggaga uggacagcuc cuacgaguuc     2820

aucuucagcc uguacaagga cgaccuccug cuggugcaga agaaggagau ggccgagccc     2880

gagcucugcu acuucguguc cuucgacacc agcggcgccu ccaucaaggu cgcgaagcac     2940

gacaacaacu ucagcacccu gaccgagaac cagaagcugc uguucuccaa cgccaccaag     3000

gaggaggugg uggggaaguc caucggcauc cagaaccuga aggucuucga gaaguggcag     3060

gucuccgugc ugggggaggu gaagcgcacg cagagcuacc cccgggagaa caucucccuc     3120

gccagcaagg ccaagaagug a                                               3141


<210>  1465
<211>  3144
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1465
augaagcugc uccgcuacau ccugggccug gaccucggga ucgccagcgu gggcugggcc       60

aucgucgaga ucgacgagaa cgacuucccc auccggcuga ucgacaucgg ggugcgcacc      120

uucgacaagg cggagguguc caagaccggc gagagccugg cccucgcccg gcgccuggcc      180

cgguccacgc gccggcugau ccgccggcgc gccuuccggc uccugaaggg gaagcgccug      240

cucaagcagc acaacgucau caacaaggag gcgcugacca cccugccgac cgacugcugg      300

gcccuccggg ugaagggccu ggacgagcgc cugagcccca cggagugguc cgccgugcuc      360

cugcaccucc ucaagcaccg gggcuaccug agccagcgca agaacgaggc caaguccgcg      420

gacaaggaga ugggggcccu gcucagcggc gucgccucca accaccagcu gcugguggag      480

aaccaguucc ggacccccgc cgagaucgcc gugaagaagu ucgcgcucga ggaggggcac      540

auccgcaacc agcugggcgc cuacacccac accuucaacc ggcuggaccu ccuggccgag      600

augaagcugc uguuccagac gcagcgcgag cuggggaacc cccacaccag cggccagcug      660

gagcaggagu ucugcgagcu ccugaugugg cagaagccgg cccuguccgg cgacgccauc      720

cucaagaugc uggggaagug caccuucgag accaacgagu acaaggcggc caagcacagc      780

uacacggccg agcgguucgu cuggcugacc aagcucaaca accugcgcau ccuggagcac      840

ggcuccgagc gcgugcucaa caccgacgag cggagccugc ugcucaacca gcccuacgag      900

aaggccaagc ugaccuacac ccaggugcgc aagcugcugg ggcucuccga ggaggcccgg      960

uucaacggcg uccgcuacgg caaggagaag gcggaggacg ccacgcugau cgagcugaag     1020

gccuaccacg ccauccggaa ggccguggag aaggcggaca agagcgccug ggagacccuc     1080

aagaaccaga ccgcccugcu ggacgagauc gggacgaccu ucuccaucua caagaccgac     1140

gacgacauca ccgagcagcu caacggcaag cugagcgaca cggugcugaa cgcccuccug     1200

gagaaccuga acuucgacaa guucauccag cucucccuca agagccugua caacaucguc     1260

ccccucaugg agcaggggcu gcgcuacgac gaggcgugcc agcagaucua cggcgaccac     1320

uucgggaaga aguccggcga gaucaaccgg cugcugcccc cgauccccgc cgacgagauc     1380

cgcaaccccg uggugcugcg gagccugacc caggcccgca aggucaucaa cgccgugauc     1440

cggcucuacg gcagccccgc ccgcauccac aucgagaccg cgcgggagcu ggggaagucc     1500

uacaaggacc gcaaggagau cgagaagcgg caggagcaga accgcgccga gcgggagaag     1560

gccaccgccc aguucaagac gcuguucccg accuucaucg gcgagcccaa ggggaaggac     1620

auccucauga ugcgccugua cgagcagcag aacggccagu gccuguacag cgggaagccc     1680

cucaaccuga accggcugcu cgagaagggc uacguggaga ucgaccacuc ccugcccuuc     1740

agccgcaccu gggacgacuc ccagaacaac aagguccugg ugcucgccgg cgagaaccag     1800

aacaagggga accagacccc guucgagugg cugggcaagg acgagcacca gugggcgcug     1860

uucguggccc gcgucaacgg gugccgguuc cccuacgcca agaagcagcg cauccucacg     1920

cagaagcugg acgagcaggg cuucaucgag cggaaccuga acgacacccg cuacaucgcc     1980

cgguuccucg ugaacuucgu ccgcgagaac cugcaccuga ccgggaaggg caagaaccgg     2040

guguucgcca gcaacggcca gaucaccgcg uuccuccgcg ggcggugggg ccuguccaag     2100

gugcgcgagg agagcgaccg gcaccacgcc cuggacgccg ucgugguggc cugcuccacg     2160

gucgcgaugc agcagaagau cacccgcuuc gugaaguacc aggaggggaa ccuccagacc     2220

gccgagcgga ucgaccgcga gaccggcgag aucauccccc ugcacuuccc cgacccgugg     2280

cacuucuucc ggcaggaggu gaugauccgc aucuucagcg agaaccccca ggaggaccug     2340

aucauccagc uccccgaccg gcccgaggcc aaccacgagu ucguccagcc gcuguucgug     2400

ucccgcaugc ccacccggaa gaugagcggc caggggcaca uggagacgau caaguccgcc     2460

aagcgccugg acgagggcau cagcgugucc aagguccccc ucacccagcu gaagcugaag     2520

gaccucgaca acauggugaa ccgggagcgc gagaaggccc uguacgaggc gcugaaggcc     2580

cgccucaacg ccuucaacga cgaccccacc aaggccuucg ccgagccguu cuacaagaag     2640

ggcgggcagc aggucaaggc ggugcgggug gagcaggucc agaaguccgg ggugguggug     2700

cgcaacggca acggcgucgc cgacaacgcc agcauggugc ggguggacgu cuucaucaag     2760

ggcgggaagu acuuccuggu gcccaucuac accuggcagg ucgccaaggg gauccugccc     2820

gaccgcgccg ugguggcggc caaggacgag agcgaguggg agcucaugga caagacggcc     2880

caguuccugu ucagcauguu cccgaacgac cugauccagg ugaagaccaa gaaggagacc     2940

uucuucggcu acuacaacgg gcucgaccgg gccaccggcg cgaucaaccu gaaggagccc     3000

gacuccaaca ucaacaaggg caagagcggg cugcaccgcu ccaucggcgu caagacggcc     3060

cucagcgugg agaaguacca gguggacgag cuggggaaga acauccggcc cugccgcccc     3120

uccaagcggc aggccguccg cuga                                            3144


<210>  1466
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1466
augaagcgcc ugggccucga caucgggacc aacagcaucg gcuggugccu gaucgaggac       60

gacggggacg gcaaggggcg gaucaucgac aucggcgccc gcaucuucuc cgacggccgg      120

gaccccaaga gcggggccuc ccuggcggug gaccgccggg ccgcccgcgc caugcggcgc      180

cggcgcgacc gguaccucgg ccgccggacc gccuuccuga aggcgcugaa gcgcuucggg      240

cucaugccgg ccgacgagag cgccgccaag cugcuggcgg accgggaccc cuacgagcuc      300

cgcgcccggg cccuggacga gcgccuggag ccccacgaga ucggccgggc ccucuuccac      360

cugaaccagc gccggggguu caaguccaac cgcaaggccg agcgcaagac gaaggacgcg      420

gaggacggca agaucgccaa cggcgccaag gcccuggacc aggccauggc ggagagcggg      480

gccgagaccc ucggccaguu ccuguccagc cgggacacca agcgcguccg gauggggucc      540

gacgcccagg gcuacgacuu cuacccccag cgccggcacg uggaguacga guucgacacc      600

aucuggagcc gccagcagca gcaccacccg gcccugcuca cggaggaggc ccggaagaag      660

cugcacgaca uccuguucuu ccagcgcccc cucaaggagc aggagguggg cgucugcacc      720

uucgugcaca acgagugccg gcugcccaag gcgcaccccc uguuccagga gcgccggcuc      780

uacgaggagg ugaaccagcu ggagaucacc accccggggg cccccaaccg caagcugacg      840

auggacgagc gggacgcccu cauccuggag cugcgcuccc ggaaggagcg cagcuucgac      900

ucccucgcca agcggcugaa gcuggccccc ggccagagcu ucaacaaggc guccgagagc      960

cgcacccggc uccgcgggga cgaggucuac gccgugaugg cccacaagaa gcgcuucggc     1020

gaccgguggc cccaccugga ccgcgaccgg cagugggcca ucaucaagcg caucaucgag     1080

gaggaggacc cggacaagcu gcacgcguuc cucaccgggg aguggggccu gaccgacgag     1140

caggcccugg ccacggcccg ggcgccccuc cccgagcugu acggccgccu ggggcccacc     1200

gccaccgccc ggauccucga ggagcugaag aaggacguga ucaccuacga cgaggccguc     1260

cgccgggccg gcuggcacca cuccgacgac cgcacggggg agauccugcc gcagcucccc     1320

uacuacggcg agcugcugac ccgggacauc ccgcccggga cccaggaccc caacgacccg     1380

cccgagcagc gcuggggcaa gaucaccaac cccacggugc acaucggccu ccggcagcug     1440

gagaagcugg ugaacgccau caucaagguc cacgggcgcc ccgaccagau cgugguggag     1500

cuggcgcggg agcucaagcu gaacgagaag gacaaggagg agcacaaccg ccggauccgc     1560

cgggacaccg ccgccgccca gcugcgcagc gccaagcucc aguccgaggg cauccccgac     1620

accggggcga accggaugcu gcugcgcauc ugggaggagc ucaaccccgg cgagguccug     1680

gaccgccggu gccccuacug cggggagccg gugggcaugc gcaugcuguu cgccggcgag     1740

gccgacaucg accacaucau ccccuacagc cggagccucg acgacuccgc cgggaacaag     1800

guggucgcgc accgcagcug caaccgggcc aagggcaacc gcacccccua cgagaagugg     1860

gugcacgacc cggaccggug ggacgugauc uccacgcagg ucgcccgccu gcacaagagc     1920

aagcaguggc gguucgggcc cgacgccaug gcccgcgugg agaaggacgg cggcuucauc     1980

gcgcggcagc ugaccgacac ccaguaccuc ucccgccugg ccgggcggua ccugagcucc     2040

cucuacccca ccaaggacga cggcagcgug uacgucaucc ccgggcgcau gacggccaug     2100

cugcggcgcc gguggggccu gaacucccuc cugccggacc acaacuucgu ggacaacgag     2160

cacagcaacg cccccaagaa ccgccuggac caccggcacc acgccaucga cgcggccguc     2220

guggccguga ccacccgcgc ccucaugcag gagaucgccc gcaccgcggg gcgggccgag     2280

gacaaggacc uggaccgccu guucgaggac cucccccagc ccugggaggg cuuccgggag     2340

gagcugggcg agaagcuggu ccgcgugacg gugucccaca agccggacca cgggcggaag     2400

ggccucccgc ccaagaaccg cgacgucacc gccgggcggc ugcacaacga caccgccuac     2460

ggccugaccg gggacguggc ggccgacggc aagacgccca ucguggucca ccgcgugccc     2520

cucaugagcc ugaagcccgc cgacaucucc gaccccgacc ggauccccga cccggcccug     2580

cgcagcgccc uccaccgggc gaccgacggc cuguccggga aggccuacga cgccgcccug     2640

gccggcuucg cgaagaccca cccccaguuc gccgggaucc gccgggugcg cguccgggag     2700

ccccucaccg ugauccccau cgccgaccgc gacggccggc cguucaaggc cuacaagggg     2760

gacgccaaca gccgcgugga caucuggcgg augcccgacg gcaaguggcu guccgacguc     2820

gugaccaugu acgaggcgca ccgccgggac cacagcgacc gccgccccca ccccgccgcc     2880

aagaagcuga ugucccuccg gcagaacgac augaucgccg uggagcgcga cggcgccccg     2940

cggcagauca uggucgugaa gcagaucugg cccacgcaga ucaaccuggu ggagcacaag     3000

gagaccggga accugaagga ccgccacagc aacgagaccg accccuuccg guucuucgcg     3060

ccgaccgccg gcgggcucaa gaagaugaag gcccgccagg uccggaucga cccccugggc     3120

cgcaucuucg accccgggcc ccgguga                                         3147


<210>  1467
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1467
auggccaaca ucgccgagaa ccugguguuc ggcaucgacc ucgggaucgg cagcugcggg       60

ugggcggucc ugaaccagaa ggccaaggac gagaacaucg uggcccuggg cuccuggugc      120

uucgacgugc ccgagaccga caaggagcgc aucccgacca accagauccg gcgcgccaac      180

cggcuccugc gccgggucau ccgccggcgc cggaaccgca uggccgagau ccggcgccug      240

uucgcgagca aggggaugcu cgacacggcc gaccccgacg cccuggccaa gaucuccgcg      300

gaccccuggg agaugcgggc caagggccug gacuaccagc ucacgcccca ggaguucgcc      360

guggcccugg gccacaucgc caagcgccgg ggguucaaga gcgcggccaa gcgcgugucc      420

accaacgccc cgagcgagga cuccaagaug cuggccgccc ucgagaagac caaggagcag      480

agcgcgcagu accggaccau cggcgagaug uucgcccgcg accccgccua cgccgaccgg      540

cgccggaacc gcgacgggug cuucgaccgc accauguccc gggaggaccu guucaaggag      600

acccagaucc uguucgagag ccagcgccgg cucggcaacu ucuccgccac cagcgagacg      660

gagcaggcgu ucaucgacau cgccuuccgc cagaagccca ugcaggacuc cgagcggcug      720

gucgagaccu gccuguucga gcccgacgag aagcgcgcca gccgguucgc cccguccuuc      780

gagcgcuucc ggcuccugug ccgccuggug aaccuccggg ugaccgacgg gcuggaggac      840

cgcccccuga cccccgagga gcuccggcug gccugcgagg acgcgggcaa gacgcgcaag      900

cugagcguca agaaggugcg gucccucauc ggccugcgcg acgaccagcg guucaccacc      960

aucccgcccg accaggagca gcacgacauc gccgcccgca ccggggacgc ccugacgggc     1020

accgcgaccu uccgggccau ccucggggag agcuucuggc gccagcugcu gccccacccc     1080

gagcagcucg accgcgccgc cuggaucauc uccuuccacg agcugaccga cacgaucauc     1140

agcaagcugg ccaccaucgg ccuccccagc gaggugaugg agcugauccg gaaggcgcug     1200

gaggacggca aguucuccaa guucaagggg gccgccagcc ucuccgacaa ggccgcccgc     1260

aagcugaucc cguuccugca ggagggccuc accuacgaca aggcgugcca gaaggccggg     1320

uacaaccacg ccgccagcaa guucacccac uacgacucca ucgacacgaa ggccaaguuc     1380

aacagccuga uggacgacgu ccgggacucc aucaccaacc ccguggcgcg caaggccauc     1440

agcgagggca ugaagcaggu cugggccaug cggaaccagu gggggcugcc cggcgccauc     1500

ugcaucgagc ucgcgcgcga cgugggcaac uccgccgaga agcggcgcga gaucgagagc     1560

gggaucaaga agaacaccgc ccagcgggag cgcgagcggc aggaggccaa ggagcaccug     1620

aaccaguccg acaucagcuc cgagacccug cuccgcuacc ggcuguggaa ggagcagggc     1680

gggcgcugcu gcuacacgga ccagcccauc accccgagcc agcugaucgc cuccgacaac     1740

agcguccagg uggaccacau ccugcccugg ucccgguucg gcgacgacag cuaccucaac     1800

aaggcgcugu gcaccgccaa ggccaaccag gagaagaaga accgcacccc cugggagugg     1860

auccacaacc agaagggggu ggaggccugg aaccacuuca cggccgcggu ggagucccgg     1920

aaggagaccc gcggccugaa gaagcggaac uuccuccuca agagcagcaa ggagaccgag     1980

gagcgcuucc ggucccgcaa ccugaacgac acccgcuacg ccgcccgggu gauggccgag     2040

gccguccgcc ugcuguaccc cagcggcaac cgggcggaga agggcggggu gaagcgcgug     2100

uucacgcggc cgggggcccu gaccgccgcc cuccgccacg cguggggcgu cgagucccug     2160

aagaagauca acgggaagcg gcugaacgac gaccgccacc acgcccucga cgccgccgug     2220

gucgccgcgg ugagcgagaa ggagauccag aagcugacca aguccuucca gaucugcgag     2280

cagaagggcc uggcccggcc caugcgcgac gugcccaugc ccugggaggg cuuccggcac     2340

cagcucgaga ccgccuacgc cgacauccgc gucgcccggc cggagcgccg gcgcgcgcgg     2400

gggaagggcc acgacgccac cauccgccag cugcgggagg agaaggacgg gcccguggug     2460

uacgagcgcc gcgccaucag cgaccugaag cucacggacc uggagcggau caaggacccc     2520

gagcgcaacu ccgccaucau cagcucccug cggaccugga ucgagaaggg caagccggcc     2580

gacuacccgc cccagagccc caagggcgac gagauccgca agauccgggu caugucccgc     2640

aacaagcccg cggugagcgu gcggggcggg accgccgacc gcggggagau gacccggguc     2700

gacguguuca cgaagauggg caagaagggg aagcccgagu gguuccucgu gcccaucuac     2760

cgccaccagg ucaucgaccg gauccagugg ccccagccgc ccaaccaggc cgccgccaac     2820

ggcaaguccg agaagaacug gcccaccaug ggccuggagc acgaguuccg cuucagccug     2880

uacccccggu ccuacgugca gaucgugaag cgcgacgggg agaucaucga gggcuacuuc     2940

gcggggcucg accggaccgu cgccgccuuc agccugcugu ccagcagcga cccgaccucc     3000

auccagcgcg gcaucggggc caagacgcuc gagagcaucc ggaaguacaa cgugaaccgc     3060

uucggcgaca ucuccauggu ggagcaggag acccggaccu ggcacggcga cgucugcauc     3120

agccccgacc agcccgacuc cgcguga                                         3147


<210>  1468
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1468
augguggaga agauccuggg caucgaccuc gggaucagcu cccugggcug ggccgucgug       60

gaguacgaca aggacaacga cgagaacaac aagaucaucg acugcggggu gcgccuguuc      120

accgccgcgg agacccccaa ggagaaggag agcccgaaca aggcccggcg cgacgcccgg      180

ggccuccgcc gggucaucaa gcgccggcgc gugcggauga acacgaucaa gaaccugcug      240

aucaccuaca agcucaucga caagacccug cuggacgagg agauggggau guuccacucc      300

cagagcaacc gcguggacgu cuggaagcuc cggcacgacg cccuguacca ccuccugucc      360

ggcgacgagc uggcccgcgu gcugauccac aucgcgaagc accggggcua caaguuccuc      420

ggggacgacg agagcgacga ggaguccggc aaggugaaga aggccggggc cgagcugaag      480

aagaaguucc uggaggccgg cugccagagc gucggggagu ggcucuggaa ggagcgcggc      540

cugcagggca agaagcggaa caaguccggg gacuacgaga ucagcauccc ccgcgacuuc      600

cugguggagg agauccagcg gaucuucgag acccagcaga aguucggcuc cacguucgcg      660

accagcgagc uccagaaggc cuacaccgac aucgccuucu acgugaagcc caugcagucc      720

aucgaggaca uggucgggua cugcaccuuc uaccccaaga ucaagagcaa gaaccaggac      780

ggcgagaagc gcgccccgaa ggccuccccc agcgcggagc aguucgugau ccuguccaag      840

aucuucagca cgaucgugau cgacgagaac aagcaggaga agaagcugau cgagcucaag      900

uccaucgagc agcugaucca gaucgcccgg agcaaggaga cccugaagua caagcagcuc      960

cgcaaggagc ugaaccuggc caaggacauc agcuucaagu ccaucagcga cgaggagaag     1020

accuggauca accucgucgg caacgccaag uucaagaaga uccuggggcu gaacuacgag     1080

accuuccuca agaacacgga gaucuccgac gagaucgcca agauccugac cuacgacaag     1140

accuucgagc agaaggagac caagcugaag aaccuccugg ugaacaucga cuggaucgac     1200

aacaaccaca ucgcggagcu ggccaagcuc agcuucuccc aguucaacca gcugagccug     1260

aaggccauca agaucaucuc caagaucaug aucgagggcu acgcccggua cgacgaggcc     1320

gugcaguacg cguucgagaa caaccuccug cccaagccca gccacgagaa guccauccug     1380

cucccgcccc ugaaggagac gaacaucgcc auccugaacc ccaccgucau ccgcgccuuc     1440

gcccaguucc gccagguggc caacgcgcuc gugagcaagu acggguccuu cgacaagguc     1500

cacuucgagc uggcccggga ggugaacacc aaggaggacc gcaagcggug ggagaaggac     1560

cgcgacaaga acgagaagau gcaccggcag aucaccgaga agcuggugga ggagggcguc     1620

aagcccagcu acaagaacau ccugaagucc aagcuccgca gcgagcagaa ggacacgugc     1680

ccguacugcc agaagaaccu gcacuacccc augaucuucg aggacgggua cgccgagauc     1740

gaccacaucc ugccccucuc ccagagccag gacgacuccu acgugaacaa gguccuggug     1800

cacagcgccu gcaaccagaa caagaagaac cggacccccu ucgagugguu ccaggacgag     1860

aagaaggacu gggacaccuu caaguccuac auccugaugg agagcacccu cggcgagaag     1920

aagcgcaacu accugaucaa ggagaacuuc agcgacccgc agucccggaa ggaguucauc     1980

agccgcaacc ugaacgacac gcgguacaug uccaaggcga ucaagaccua cugcgagaac     2040

cacuggaagc ucagccacga cgacgacaag cugcgcaucc aggugcgguc cggcaagcug     2100

accagcaccc uccgccacca gugggggcug gacaacaaga accgggagac gcacacccac     2160

cacgccaugg acgccaucau gaucgccuuc uccacccagg gcauggucaa gaagcugagc     2220

gacuacuucg ccaagaagga ggcgaaggug gagaaggaca agcccgugcu caucaccccc     2280

aucaagcagu ucaaggaggc cgucgagcag gccacgaccc uggagcgcca ggaguccauc     2340

cagaccaagg ccggggacac caucacgcug aaccggcucc ugaucagccg gcccccgcgc     2400

gccuccguga ccggcgcggc ccacgagcag accgccaagc ccuacccccg caucaagccc     2460

aucaagaaca aguacaagcg ccggcgcauc ccgaucgacg aggacaaguu cgagcuguuc     2520

cggaacgaca agguggccag cgggaacgac aagaacuucu acaacuccag caccaucccc     2580

cgcgucgaca ucuacaagaa ggacgacaag uaccacgugg ugcccaucua ccucuccgac     2640

augaccaagg ccgagguccc caacaagagc cugggcacga acccggaggg cauggacgag     2700

aaguacuucu gcuucuccgu guucaagaac gaccugaucg agcucgagac caaggcgacc     2760

cccaagaagc ccagcaagaa gcugcugggg uacuucaagc agcucaacgg cgccaacuuc     2820

auccugaaca gcauccacaa cgggaucauc gacggcuucg ugugcucccc gaucacccug     2880

uucaagcagc agaaggacau gugcaagaag ugccuccccg aggaccgggc caucgggaac     2940

ugcagccagg agacgcugga guucugggag gccgagaaca ucaagguccc caagaaggac     3000

uucgagugcg accagggcau caaguucgcg aucgccgugc gcaaguacac caucgacccc     3060

cugggcuacu accacgaggu gaagggggag aagcuccugg gcaccauccc gcagggggcc     3120

aagaagcacc ccaagcggca gaaguga                                         3147


<210>  1469
<211>  3150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1469
augaucaacg agcgccugac cuucggcauc gaccucggga ucggcagcug cgggugggcc       60

gugcugcggc acgccgacgc ggccggcgag cccgggguca ucgagggccu gggcuccugg      120

ugcuucgacg ugccggagac cagcaaggag cgcaucccca cgaaccaggu gcggcgcucc      180

aaccggcucc ugcgccgggu cauccgccgg cgccggaacc gcauggccga gauccggcgc      240

cuguuccacg cccaggggcu ccugcccagc gccgacgagg acgcgcugaa gcggccccgc      300

cucgacccgu gggagcugcg ggcccgcggc cuggaccagu gccucuccgc cgaggaguuc      360

gccguggcgc uggggcacau cgcccggcac cgcggcuucc ggagcuccgc caagcgcaag      420

gccgccaaca ccagcgggga ggacuccaag augcugagcg cgcuccuggu gacccgcgag      480

cggaccgccc gcuaccggac ggucggcgag auguucgccc gcgaccccga guacuccgcc      540

cggcgccgga accgcgacgg caucuucgac cggaccgugg cccgcgacga ccugauccac      600

gaggugggga agcuguucga ggcgcagcgg gcccgcggca gcgccuucgc cucccccgac      660

cuggaggccg acuucaacgc gaucgccuuc cggcaggccg agaugcagga cagcgagcgc      720

cuggucggga acugccccuu cgagcgggac gagaagcgcg ccgcccggug ggcgccgucc      780

uucgagcgcu uccggcuccu gacccgccug aucaaccucc gcaucgccac cgccgagggc      840

gagcggcgcu ucacgcccga ggaccuggcc cgggugagcg cggagcuggg cgccaccgcc      900

aagcuccccg ugucccgcgu ccgggcccug aucgggcugc uccccgacga gcgcuucacc      960

accauccggg uggaggacga ggaccgcgac aucguggccc ggacgggcga ggcggccccg     1020

gggaccuggc gccugcggaa gacccugggc gaggcccucu gggccgagau gcaggcccgc     1080

cccgaccagc uggacgcgau cgcccacguc cugagcuucu ucgaggccac cgccaagauc     1140

cgguccgagc ucgaccgccu ggggcugccc gccgcgguga ucgaggcccu cgaccggggc     1200

cuggaggacg gcgccuucgc caaguucaag ggggcgggcc acaucagcgc caaggccugc     1260

cgcaaccugc ucccccaccu ccagcagggg caccgguacg accaggccug ccgccuggcc     1320

ggcuacgacc acgcggccag cacgcucucc cacaccgccc agguggucag caaggccggg     1380

uucaacgccc uggugaccga gcugggcgag uccaucgcga acccgaucgc ccggaaggcc     1440

cucaccgagg gccugaagca gauccgcgcc augugcgccc ggugggggcu gcccggcgcg     1500

auccacaucg agcuggcccg cgaggugggg aacagcaucg agaagcgccg ggagcucgag     1560

ucccagcgcg agacgaccac caagcggcgc cagaccgagc gggacgagau ccgcggccug     1620

cuggcccucg aggacgucga cgacgacacg cugcugcggu accgccucug gaaggagcag     1680

ggcgggaagu gccuguacac cggcaaggac auccacgugc ggcagcugau cgccaccgac     1740

aacagcgugc aggucgacca cauccucccc uggucccgcu ucggggacga cagcuucaac     1800

aacaagaccc ugugccuggc guccgccaac cagcggaagc gcggccagac gcccugggag     1860

ugguucagcc ucaccgggac cccggaggcc ugggaggugu ucgccucccg ggucgagacc     1920

agcaaggacc ugcgcggcuu caagaagcgg aaccugcucc ugaagaacgc cgacgaggug     1980

gcgcagcgcu uccggucccg caaccugaac gacacgcggu acgccgcccg ccuccuggcc     2040

gaggccgcga agcuguucua ccccgccggc gaccgccagg agaaggacgg gcggcgccgg     2100

gugcucaccc gccccggccc ccugaccgcc gcccugcggc acgccugggg gcucgagagc     2160

cugaagaagg cggacggcaa gcgcgucucc gacgaccggc accacgcccu ggacgcccuc     2220

gccguggccg cgaucagcga gcgcgaggug cagcggcuga cccgcuccuu ccaggagugg     2280

gagcagcagg ggcuggcccg gccgcuccgc cacgucgccc cgcccuggcc cggcuuccgg     2340

ggcgacgugc uggccgcgua cgcccaggcc uucguggccc gccccgagcg gcgccgggcc     2400

cgcgggaagg gccacgacgc gagcauccgg caggucgugg gggagggccc cgaggcccag     2460

guguucgagc gccgggggcu ggccgaccuc aaggaggccg accuggaccg cgucaaggac     2520

cccgagcgca accaggccau caucgaggcg gugcgggccu ggauccuggc cggccgcccg     2580

gccgacgccc cgccccggag cccccgcggc gacgugaucg ucaagcuccg gacggcgccc     2640

gccaagaaga ucaagcccgc cgugcccgug cgcgggggca ccgccgaccg cggggacaug     2700

guccggaucg acguguucac ccggcccaac cgcaagggcc gggacgagug guaccuggug     2760

ccgaucuacc cccaccaggu caugaaccgc aaggcguggc cccacccgcc caugcggagc     2820

gugguggccu acaaggacga ggccgggugg accgagaucg gcgccgagca cagcuuccgc     2880

uucucccugu acccccggag cuacguccag cucgugaagu ccaacggcga cgugaucgag     2940

ggguaccugg ucagcaugga ccgcuccacc ggcgccuucc ggguguucgu gcacaacaac     3000

cccgcggaga uggccaaggg gaucggcgcc aagacgcugc ucaaccugcg caaguucaac     3060

gucgaccggu ucggggugcg caccgaggug aaguccgagg uccgcaccug gcacggcgag     3120

gugugcaccu cccccgugcc gcccgacuga                                      3150


<210>  1470
<211>  3150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1470
augcagcuga gcaagcucaa cuacauccug ggccuggacc ucgggaucgc cuccgugggc       60

uggagcguca ucgagaucga cgagaccgag augcccaucc gccugaucga cgugggggug      120

cggaccuucg agcgcgccga ggucccgaag acgggcgagu cccuggcgcu cgcccggcgc      180

cuggcccgga gcacccgccg gcugauccgc cggcgcgccu uccggcuccu gaaggccaag      240

cgccugcuca agaaccacca gaucgugucc gcggacgagc ugagcacccu gcccaaccac      300

ugcugggagc uccgggugaa ggggcuggac gagcgccuga ccugccagga gugggccgcc      360

guccuccugc accuccucaa gcaccggggc uaccuguccc agcgcaagag cgaggcccag      420

aacgcggaca aggagauggg cgcccugcuc uccggggugg ccaacaacca caagcugcug      480

agcgagaacc acuaccggac gcccgccgag aucgccguga agaaguucgc gcucgaggag      540

ggccacaugc gcaaccagcg cggggccuac acccacaccu ucaaccggcu ggaccugcuc      600

gccgagaugc ggcugcuguu ccagacccag cgcgagcucg gcucccccua cgccagcggg      660

gagcuggagc aggaguucug caagcugcuc auguggcaga agccggcccu guccggcgac      720

gcgauccuga agaugcucgg caagugcacg cuggagccca ccgaguacaa ggccgccaag      780

aacaccuaca ccgccgagcg cuucgucugg cugacgaagc ucaacaaccu gcggauccug      840

gagaacggga gcgagcgcgc ccucaacgcg gacgagcggg cccugcugcu caaccagccc      900

uacgagaagg ccaaguucac cuacgagcag gcccgcaagc ugcugcagcu gaacgaggcc      960

gcgcgguuca acuccgugcg cuacggcaag gagaacgccg agaaguccac ccucauggag     1020

augaaggccu accacgccau ccggaaggcg guggagaccg ccgacagcag cgccugggcc     1080

acgcugaaga cccagccccu gcuccuggac gagaucggga ccgccuucuc caucuacaag     1140

accgacgagg acaucaagac gcagcugcag ggcaagcucg cggacaacgu caucgacgug     1200

cugcuggaga accucaacuu cgacaaguuc auccagcuga gccugaaggc ccucuacaag     1260

auccugccgc ugauggagca gggccuccgc uacgacgagg ccugcagcca gaucuacggg     1320

gaccacuucg gcaagaagga gcagaagaac gaccggcugc ugccccagau ccccgccgac     1380

gagauccgca accccguggu ccuccggacc cugucccagg cccgcaaggu gaucaacgcg     1440

gugauccggc uguacgggag cccggcccgc auccacaucg agaccgcccg ggagcucggc     1500

aaguccuaca aggaccgcaa ggagaucgag aagcggcagg agcagaaccg cgccgagcgg     1560

gagaaggccg ucgacaaguu caaggagcug uuccccaacu ucaucgggga gcccaaggcg     1620

aaggacaucc ugaccaugcg ccucuacgag cagcagaacg gccagugccu guacagcggc     1680

aagucccugg accuccaccg ccugcuggag aagggguacg uggagaucga ccacgcccuc     1740

cccuucagcc ggacguggga cgacuccuuc aacaacaagg ugcugguccu ggccggcgag     1800

aaccagaaca agagcaacca gaccccguuc gaguggcucg ggaaggacga gcagaagugg     1860

gcccuguuca ccgcgcgcgu gaacuccugc cgguuccccu acagcaagaa gcagcgcauc     1920

cugaccaaga agcucgacga cgagggcuuc auccagcgga accugaacga cacgcgcuac     1980

aucgcccggu uccuggugaa cuucgucaac gacaaccucc accugaccgg gaagggcaag     2040

cgccgggugu ucgccuccaa cggccagauc accgccuucc ugcgcgggcg guggggccuc     2100

accaaggucc gcgaggagag cgaccggcac cacgcccugg acgcgguggu ggucgccugc     2160

uccacgguga gcauccagca gaagaucacc cgcuucguga aguaccagga ggggaaccug     2220

cagaccgccg agcggaucga ccgcgagacc ggcgagauca ucccccucca cuuccccgac     2280

ccgugggccu ucuuccggga ggaggucaug auccgcaucu ucuccgagau gccccaggag     2340

gaccugguga ucaagcugcc cgaccggccc caggccaacc acgaguucgu gcagccgcug     2400

uucgucagcc gcaugcccac gcggaagaug uccgggcagg gccacaugga gaccaucaag     2460

agcgcgaagc gccuggacga gggcaucucc gugagcaagg ugccccugac ccagcucaag     2520

cugaaggacc uggagaacau ggucaaccgc gaccgggaga ucgcccucua cgaggcccug     2580

aaggcccgcc ugaccgaguu caaggacgac cccgccaagg cguucgccga gccguucuac     2640

aagaagggcg gggugcaggu caagagcgug cggguggagc agguccagaa guccggggug     2700

guggugcgca acggcaacgg cgucgccgac aacgccagca uggugcgggu ggacgucuuc     2760

aucaagggcg ggaaguacuu ccucgugccc aucuacaccu ggcaggucgc gaaggggauc     2820

cugcccgacc gcgccgugau cgccuacacg ccggagaacg agugggagcu gauggacgac     2880

uccgcccagu uccaguucag caucuacccc aacgaccuca ucaagcugac caccaagaag     2940

gccaucuucu ucggcuacua cuccggguuc aaccggcuga ccggcgcgau caacaucaag     3000

gagcacgacc uccagaucag caagggcgac aagggggugc accugggccu gggggugaag     3060

cucgcccugu cccuggagaa guaccagguc gacgagcucg gcaagaacau ccgccccugc     3120

cggcccacgc agcgccagaa cgugcgguga                                      3150


<210>  1471
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1471
augagcuccu accgcuucag cuucgaccug ggcaccaacu ccaucgggug ggccguguuc       60

gagcucgacc ccaagacccg gccggucgcc cuggcggacu gcggcgugcg caucuucccc      120

gacgggcgga accccaagga caagcagagc cuggccgugg cccgccggga ggcccgcgcc      180

guccggcgcc ggcgcgaccg guaccucaag cgccgguccg agcugaugga gcgccuggug      240

gcgaagggcc ucaugcccga gagccgggac gaccagaagg cccuggaggg gcuggacccg      300

uacgcccucc gcgccgcggg ccuggacgac gcccugccgc cccaccacuu cggccgggcc      360

cucuuccacc ugaaccagcg ccggggguuc aaguccaacc gcaagaccga ccggggcgag      420

aacagcgaga ccgggaucac caagaucggc augaagaagc ugcacgaggc ccuccugucc      480

gaggggugcc gcacccuggg cgagcuccag caccgccacc acgaggccaa ccagacgccc      540

gcgauggaca ccaaccccaa gagcgugcgg uccaagcugc gcggcgaggg ggccaaggcc      600

gucuacgacu ucuaccccag ccgggacgcc guggaggacg aguucaacaa gcugugggac      660

ucccagaagc gcuuccaccc cgaccuccug accgacagcg cccgggacgc gauccgcgac      720

gccaucuucu accagcggaa gcugaagacc cccgacgccg gccgcugcuc cuucaacccg      780

gccgaggagc ggguggcccg cgcgaugccc aucgcccagc acuuccggau ccuccaguuc      840

gucggggacc ugcgccugau cgacaacgac cucgugcggc acccccugaa caacgaccag      900

aagaaggccc uguaccgcga ccuccuggcc aagggcaagc ugacguucaa ggccaugcgg      960

aagaccaucg gcaucgggag cgaguacgcg uucucccacg agagcgagaa gcaguccgag     1020

uuccgcggcg acgagaccgc cgcccggcuc gccgggccca aguacuucgg cccgaagugg     1080

cgcgagcuga gcuucaacga ccagagcgag aucguggacc ggcugcucca cgaggagaac     1140

gaggagaccc ugauccagua ccugcaggac cgcuucgggu ucgaccacga ccgcugcgag     1200

gucaucgcgg acgcgccccu ccccgacggc cacacgcggc ugggcaagac cgcccuggag     1260

acccucgugg agaucaugcg cgacgaccac cuccccuacg accaggccug caccaaggcc     1320

ggguacgagc acgcccugcc gcccgacggc gugauccugg acgaccugcc cuacuacggg     1380

aucgcccugg agcggcacgu cgccuucggc gacgggaagu ccgagaaccc cgagaagcgc     1440

uacggcaaga ucgccaaccc gacggugcac aucgcccuca accagcugcg gaaggugauc     1500

aacgcgcuga ucgcccgcua cggccacccc gaccaggucg ugauggagau cgcccgggac     1560

cugaagcaga gcaaggccaa gcgcgacgac aucuccaagg agcagaagaa gaaccaggac     1620

cggaacgacc agcgccggaa gaagcuccag gaggcccaca ucgcggugaa cgccgagaac     1680

augcugcgca ugcggcugug ggaggagcuc gacgaggaca acgccgccgc gcgcgccugc     1740

cccuucaccg gggagaccau cagcauggcc cggcuguucu cccccgaggu cgagaucgag     1800

cacauccugc cguucagccg cucccucgac aacagccccg ccaacaagac ccugugcaug     1860

gugugggcca accgggcgaa gggcaaccgc acgcccuggg aggccuucgg gcgggaccgc     1920

gagccgggcu ucgacuggcc cgagaucgcc gcccgggccg cgcgccugcc caagaacaag     1980

uccuggcgcu ucgggcccga cgccauggag cggcucgagc gcgagggcga cuuccuggac     2040

cggcagcuga ccgacaccca guaccucagc cgcaucgccc gggaguacgu gaccaagauc     2100

ugcgacccga accaggucug ggugacgccc ggccgccuga ccgccuggcu gcggcgcaac     2160

ugggggcuca acggcauccu gcccaccggg aacgccacca agaaccggga cgaccaccgc     2220

caccacgcga ucgacgccau cgucgugggc cugacggacc ggggcauccu caaccgcgug     2280

ucccggagcg ccgcccuggc ggaggccgag gaccuggacc gcuccaucgc cgagaugccc     2340

gacccguucg acggguuccg ggacaacauc cgccacaucc ucgagcagau caucgucagc     2400

cacaagcccg acgccggcuu cgugcccggg caccccaagg gcaacaccac cccgggggcc     2460

cugcacaacg acaccgcgua cggcaucauc aagacggacg ccaagggcau guccgaggug     2520

gucacccgga aggacgugcu gagcuucaag gggaccaagg agaucgaguc cauccgcgac     2580

gccgccaccc ggcagagccu ccuggacgcc guggcgggcg uccccuccgg gcuggccuuc     2640

aaggaggccc uccgcgacug gagcgugcgc acgggcacca agcgggugcg cgucaccuuc     2700

aacaugagcg ugauccccau ccaggaccgg gacgggaagg ccuacaaggg cuacaagggc     2760

gacgggaacc ugugcgccga gaucuacgag cgccccgacg gcaagugggg cgacgagauc     2820

aucucccggu ucgacgcggu gcagccgggg uucacgcccc aguggcagcg cgaccacccc     2880

gccgcccacc gggucaugcg ccuguuccgg gacgacauga ucauggccga gaucgacggg     2940

gugaugaccg ugugccgcau cgucaagaug agccccggca aggugguguu caccccgcac     3000

cacgaggcca acaccgacuc ccggaaccgc gacaaggacg accccuucag cuuccacacg     3060

ugcuccggcg agagccuccg gaagcgcaag gcgcagaagc uguucgucga cccccugggg     3120

aagauccggg gcuaccgcgc caaggagggg uga                                  3153


<210>  1472
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1472
augggcgaga acaugaucga cgagagccug accuucggga ucgaccucgg caucgggucc       60

ugcggcuggg ccgugcugcg ccggcccagc gccuucgggc gcaagggcgu caucgagggc      120

augggguccu ggugcuucga cgugcccgag accagcaagg agcggacgcc caccaaccag      180

auccgccggu ccaaccgccu gcuccggcgc gugauccggc gccggcgcaa ccggauggcg      240

gccauccgcc ggcugcugca cgccgccggc cuccugccca gcaccgacuc cgacgcccug      300

aagcgccccg ggcacgaccc gugggagcuc cgggcgcgcg gccuggacaa gccccugaag      360

cccgucgagu ucgccguggu gcucgggcac aucgccaagc ggcgcggcuu caagagcgcc      420

gcgaagcgga aggccaccaa caucuccagc gacgacaaga agaugcugac ggcccuggag      480

gccacccgcg agcgccucgg ccgguaccgc accgucgggg agauguucgc ccgggacccc      540

gacuucgcgu cccgccggcg caaccgggag ggcaaguacg accgcaccac ggcccgggac      600

gaccuggagc acgaggugca cgcccuguuc gccgcccagc gccggcucgg gcagggcuuc      660

gcgagcccgg agcuggagga ggccuucacc gccuccgccu uccaccagcg ccccaugcag      720

gacagcgagc ggcugguggg cuucugcccc uucgagcgca ccgagaagcg ggccgcgaag      780

cucacccccu ccuucgagcg cuuccggcug cuggcccgcc uccugaaccu gcggaucacg      840

accccggacg gggagcgccc ccucaccguc gacgagaucg cccuggugac ccgcgaccug      900

ggcaagacgg ccaagcucag caucaagcgg gugcgcaccc ugaucgggcu ggaggacaac      960

cagcgguuca ccaccauccg ccccgaggac gaggaccggg acaucgucgc ccgcacgggc     1020

ggggcgauga ccggcaccgc cacccuccgg aaggcccugg gcgaggcccu guggacggac     1080

augcaggagc gccccgagca gcucgacgcg aucguccagg ugcuguccuu cuucgaggcc     1140

aacgagacca ucaccgagaa gcugcgggag aucgggcuca cccuggccgu gcuggacgug     1200

cuccugacgg cccuggacgc cggcguguuc gcgaaguuca agggggccgc ccacaucagc     1260

accaaggccg cccgcaaccu ccugccgcac cuggagcagg gccggcgcua cgacgaggcg     1320

ugcaccaugg ccggguacga ccacgccgcc ucccggcuca gccaccacgg ccagaucguc     1380

gccaagaccc aguucaacgc gcuggugacg gagaucggcg agagcaucgc caaccccauc     1440

gcccgcaagg cccugaucga ggggcugaag cagaucuggg cgaugcggaa ccacuggggc     1500

cuccccgggu ccauccacgu ggagcuggcc cgcgacgucg gcaacagcau cgagaagcgg     1560

cgcgagaucg agaagcacau cgagaagaac accgcccugc gggcccgcga gcggcgcgag     1620

gugcacgacc uccuggaccu ggaggacgug aacggggaca cccuccugcg cuaccggcug     1680

uggaaggagc agggcggcaa gugccucuac accgggaagg ccauccacau ccgccagauc     1740

gcggccacgg acaacuccgu ccagguggac cacauccugc ccuggagccg guucggcgac     1800

gacuccuuca acaacaagac ccugugccuc gccagcgcca accagcagaa gaagcgcucc     1860

accccguacg aguggcugag cgggcagacc ggcgacgccu ggaacgcguu cguccagcgg     1920

aucgagacca acaaggagcu gcgcggcuuc aagaagcgga acuaccuccu gaagaacgcc     1980

aaggaggccg aggagaaguu ccgcucccgg aaccugaacg acacgcgcua cgccgcccgc     2040

cucuucgcgg aggccgugaa gcugcuguac gccuucgggg agcggcagga gaagggcggg     2100

aaccggcgcg uguucacccg gcccggcgcc cucaccgcgg cccugcgcca ggccuggggc     2160

gucgagagcc ugaagaagca ggacgggaag cggaucaacg acgaccgcca ccacgcccuc     2220

gacgcccuga ccguggcggc cguggacgag gccgagaucc agcggcugac gaaguccuuc     2280

cacgaguggg agcagcaggg ccucgggcgc ccccugcgcc gggucgagcc cccgugggag     2340

agcuuccgcg ccgacgugga ggccaccuac cccgaggugu ucgucgcgcg gcccgagcgc     2400

cggcgcgccc ggggcgaggg gcacgccgcc accauccgcc aggugaagga gcgggagugc     2460

acccccaucg uguucgagcg caaggccguc uccagccuga aggaggcgga ccucgagcgg     2520

aucaaggacg gcgagcgcaa cgaggccauc guggaggcca uccgguccug gaucgccacg     2580

gggcgcccgg ccgacgcgcc gccccggagc ccccgcggcg acaucaucac caagauccgg     2640

cuggccacca ccaucaaggc cgccgugccc guccgcggcg ggacggcggg ccgcggggag     2700

auggugcggg ccgacguguu cuccaagccc aaccggcgcg gcaaggacga gugguaccug     2760

guccccgugu acccgcacca gaucaugaac cgcaaggccu ggcccaagcc gcccaugcgg     2820

agcaucgugg ccaacaagga cgaggacgag uggaccgagg ucgggcccga gcaccaguuc     2880

cgcuucuccc ucuacccccg gagcaacauc gagaucaucc gccccagcgg cgaggugauc     2940

gagggcuacu ucguggggcu gcaccggaac accggcgccc ugaccaucuc cgcgcacaac     3000

gaccccaaga gcauccacuc cgggaucggc acgaagaccc uccuggccau cagcaaguac     3060

caggucgacc gcuucgggcg gaaguccccg gugcgcaagg aggugcggac cuggcacggc     3120

gaggccugca ucagccccac cccgcccggc uga                                  3153


<210>  1473
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1473
augaagaucc ugggcuucga caucgggauc gccagcaucg gcugggccuu cguggagaac       60

ggggagcuca aggacugcgg cguccgcauc uucaccaagg cggagaaccc caagaccggg      120

gacucccugg ccaugccgcg gcgcgaggcc cggagcgugc gccggcgccu ggcccggcgc      180

aagggccggc ucgagacgcu gaagcgccug cucgccaagg agugggaccu gugcuacgag      240

gacuacaucg cggccgacgg cgagcugccc aaggccuuca uggggaagaa ccucaacaac      300

cccuacgugc ugcgguacga ggcccugcag cgccuccugu ccaaggagga gcugguccgg      360

guggugcucc acaucgcgaa gcaccgcggc uacgggaaca agaacgccaa gaucaccaag      420

agcgaggagu ccaagcggga gcagggcaag auccugagcg cccuggccac caacgccucc      480

gcgaucgccc gcuaccggac cgucggggag uacuucuaca aggaguucug cgaggugauc      540

aagaaccccc agggccucag cacgaacgag cacuacaccc aguccaaggu gcgcguccug      600

aagccgaucc ggaacaaggg cggggaguac accaacugca uccugcagga ggaccuccag      660

cgcgagcugc ggugcaucuu cgaguaccag aagggcuucg gguuccccau cacccaggag      720

uuccaggaca agauccugaa gaucgccuuc uaccagcgcc cccucaagga cuucagccac      780

cuggugggca agugcacguu cuaccccgac gaguuccgcg cccugaagug cucccucagc      840

gccguggagu ucaucacccu guccaagacc aucaacgucc ugaccuacau cagcaaggag      900

uccggcgagg uguacagcaa ggggcaguac gagcagaugc ucgacuccgu guucaaggcg      960

gucugcgaga agggcagccu guccuucgcc cagcugcgga agauccucca caucgacgag     1020

cgcauccggu ucaaggaggu ggacuacaug gcccgcaaga gcaagaacac gagcggggac     1080

uccacccaca agcagaagac cagcaacccg gagaccaaga aguucaucga gcacaagaac     1140

cugaagaagu ucugcgagau ccuguccgag aucgccgagg accgggcccu cgcggacagc     1200

aucgcccgcg acaugacgcu gaucaaggac gaggagaagc uggcccagaa gcuccacggc     1260

uacgggaagu ucucccagga gcagaucacc cagcugagcg cccugaacuu cucccaccac     1320

aucaaccuca gccugaaggc ccuguccgag auccuccccu ucaugcggga gggcaugcgc     1380

uacgacgagg cgugcaagcu ggccggccug aaggcccggc acaacgacaa gaaguccaag     1440

uuccucccgc ccuucugcga gagcaucuac gccgacgagc ugaccaaccc cgugguccac     1500

cgcgcgaucg ccgaguaccg gaagauccug aacgcccuga ucgccaagua cgggagcgug     1560

cacaagaucc acaucgagcu cacccgcgac gugggcaaga acuuccagga gcgggagaag     1620

uacaagaagg agaucgaguc caacuacaag gcccgcgucc aggcgaugca ggagugcgag     1680

aagcuggggc ugacgcucag cgagggcaac auccugaagc ugcgccucuu ccgggagcag     1740

aacgagaucu gcguguacuc cgggcggaag aucacccugg ccaaccugaa ggagcagggc     1800

gcccucgaga ucgaccacau ccugcccuac agccgcucca gcgacgacuc cuacaugaac     1860

aaggugcugg ucuucaccaa cgagaaccag aacaagggca acaagacccc cuacgaggcc     1920

uucgggggcg acagccagaa guggggcgag aucgagagcc ucgcccugcg guccggguac     1980

cccaagaaga aggcgaagcg cauccuggac aagggguucg gcgaccgcga ggccggcuuc     2040

aagagccgga acaucgugga cuccggguac aucgcccgcc ucaucgccaa cuacacgaag     2100

gaguaccuga aguuccugcc gcucgacagc cacgagaaca ccgcccugau cgcgggcgag     2160

aaggacucca agauccacgu cgaggccgug aaggggaugc ugaccgccac caugcggcac     2220

uucuggggcc ucggcagcaa gaaccgcuac gagcacacgc accacgccgu ggacgcgauc     2280

aucaucgccu acaucaacgc cgccaugauc cagcgguucu cccaguuccg ccagaaccag     2340

gagagccuga aggcccgguu cuacgcgaag gagcuggcca aggaggaguu caagacccag     2400

cgcgccuucu ucgagcccuu ccggggguuc cgcgagcagg uccucgccaa ggugggccag     2460

aucuucgugu cccggccgcc ccgcaagcgg gcccgcgggg cgcugcacga ggagaccuuc     2520

uacagcaucg acgacaagaa gcuguccgag accuacggcg ggaaggaggg cguccagcgg     2580

gcccucagcc ugggcaagau ccgccagauc gggacgaaga ucguggccaa cggccccaug     2640

gugcgggucg acaucuucaa gcacaagcag ucccgccggu ucuacgccgu gcccaucuac     2700

accauggacu ucgcccuggg gauccucccc aacaaggcgg uggucagcgg caaggacacc     2760

aaguccaagg ugaucaagga cuggcuggag auggacagcu gcuacgaguu cugcuucucc     2820

cuguucaagg acgaccucau cgaggugcag aaguccgaca uggagaagcc cgagcuggcc     2880

uacuucguca gccuggacgc cagcgacggg cgcaucaagg ugcggcacca cugcaacgac     2940

aucaccaaga ucaacgagaa ccagaagaag cuguucagcg aggccaucga gaaggaggug     3000

gucggccgcg gcuccaucca gaaccucaag guguucaaga aguacaaggu gagcccgcug     3060

ggggagauca aggaggcgaa guacgagccc cgccagccca ucgcccucaa gacguccccc     3120

aagaagcacc ggaagcagaa caacgcccag uga                                  3153


<210>  1474
<211>  3156
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1474
augaacaacu acauccuggg ccucgacauc gggaucacca gcgugggcua cgggaucguc       60

gacuccgaca cccgcgagau caaggacgcc ggcgugcggc uguuccccga ggccaacgug      120

gacaacaacg aggggcgccg gagcaagcgc ggcgcccggc gccugaagcg gcgccggauc      180

caccgccucg accgggucaa gcaccuccug gccgaguacg accuccugga ccugacgaac      240

aucccgaagu ccaccaaccc cuaccagacc cgcgugaagg gccucaacga gaagcugagc      300

aaggacgagc uggugaucgc ccuccugcac aucgccaagc ggcgcgggau ccacaacguc      360

aacgugauga uggacgacaa cgacuccggc aacgagcuga gcaccaagga ccagcucaag      420

aagaacgcca aggcgcuguc cgacaaguac gugugcgagc ugcagcucga gcgguucgag      480

caggacuaca agguccgcgg ggagaagaac cgguucaaga cggaggacuu cgugcgcgag      540

gcccggaagc ugcuggagac ccagagcaag uucuucgaga ucgaccagac cuucaucaug      600

cgcuacaucg agcucaucga gacccggcgc gaguacuucg agggccccgg gaagggcucc      660

cccuucggcu gggaggggaa caucaagaag ugguucgagc agaugauggg ccacugcacg      720

uacuucccgg aggagcugcg cagcgugaag uacuccuaca gcgccgagcu guucaacgcc      780

cucaacgacc ugaacaaccu ggucaucacc cgggacgagg acgcgaagcu caacuacggg      840

gagaaguucc agaucaucga gaacguguuc aagcagaaga agacccccaa ccugaagcag      900

aucgccaucg agaucggcgu gcacgagacc gagaucaagg gcuaccgcgu caacaagucc      960

gggaagcccg aguucacgca guucaagcug uaccacgacc ucaagaacau cuucaaggac     1020

cccaaguacc ugaacgacau ccagcugaug gacaacaucg ccgagaucau caccaucuac     1080

caggacgccg agagcaucau caaggagcuc aaccagcugc cggagcugcu cuccgagcgg     1140

gagaaggaga agaucagcgc ccuguccggc uacagcggga cccaccgccu gucccucaag     1200

ugcaucaacc ugcugcucga cgaccugugg gagagcagcc ugaaccagau ggagcuguuc     1260

accaagcuga accugaagcc caagaagauc gaccucuccc agcagcacaa gauccccagc     1320

aagcuggugg acgacuucau ccuguccccc guggucaagc gggcguucau ccagagcauc     1380

caggugguga acgccaucau cgacaaguac ggccugccgg aggacaucau caucgagcuc     1440

gcccgcgaga acaacuccga cgaccggcgc aaguuccuga accagcugca gaagcagaac     1500

gaggagacgc ggaagcaggu cgagaaggug cuccgcgagu acgggaacga caacgccaag     1560

cggaucgugc agaagaucaa gcugcacaac augcaggagg gcaagugccu guacagccuc     1620

aaggacaucc cccuggagga ccugcuccgc aacccccacc acuacgaggu cgaccacauc     1680

aucccccggu ccguggccuu cgacaacagc augcacaaca agguccuggu gcgcgcggac     1740

gagaacucca agaagggcaa ccggaccccg uaccaguacc ugaacagcuc cgagagcucc     1800

cucagcuaca acgaguucaa gcagcacauc cugaaccugu ccaagaccaa ggaccgcauc     1860

accaagaaga agcgggagua ccuccuggag gagcgcgaca ucaacaaguu cgacgugcag     1920

aaggaguuca ucaaccggaa ccuggucgac acgcgcuacg ccacccgcga gcucaccagc     1980

cugcugaagg ccuacuucuc cgccaacaac cucgacguga aggugaagac caucaacggg     2040

agcuucacga acuaccugcg gaaggucugg aaguucgaca aggaccgcaa caagggcuac     2100

aagcaccacg ccgaggacgc gcugaucauc gccaacgccg acuuccucuu caagcacaac     2160

aagaagcugc ggaacaucaa caaggugcug gacgcccccu ccaaggaggu ggacaagaag     2220

cgcgucaccg ugcagagcga ggacgaguac aaccagaucu ucgaggacac ccagaaggcc     2280

caggcgauca agaaguucga gauccggaag uucagccacc gcguggacaa gaagcccaac     2340

cggcagcuca ucaacgacac ccuguacucc acgcgcaaca ucgacgggau cgaguacguc     2400

guggagagca ucaaggacau cuacuccgug aacaacgaca aggucaagac caaguucaag     2460

aaggaccccc accggcugcu cauguaccgc aacgacccgc agaccuucga gaaguucgag     2520

aagguguuca agcaguacga gagcgagaag aaccccuucg ccaaguacua cgaggagacc     2580

ggcgagaaga uccggaaguu cuccaagacg gggcagggcc ccuacaucaa caagaucaag     2640

uaccugcgcg agcggcuggg ccgccacugc gacgugacca acaaguacau caacagccgg     2700

aacaagaucg uccagcucaa gaucuacucc uaccgcuucg acaucuacca guacgggaac     2760

aacuacaaga ugaucaccau cagcuacauc gaccuggagc agaaguccaa cuacuacuac     2820

aucagccggg agaaguacga gcagaagaag aaggacaagc agaucgacga cuccuacaag     2880

uucaucggca gcuucuacaa gaacgacauc aucaacuaca acggggagau guaccgcgug     2940

aucggcguga acgacuccga gaagaacaag auccagcugg acaugaucga caucagcauc     3000

aaggacuaca uggagcucaa caacaucaag aagaccgggg ucaucuacaa gacgaucggc     3060

aaguccacca cccacaucga gaaguacacc accgacaucc ugggcaaccu guacaaggcc     3120

gcgcccccga agaagcccca gcucaucuuc aaguga                               3156


<210>  1475
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1475
auggagaaca agaaccugca cuacauccuc ggccuggacc uggggaucgc cagcgugggc       60

ugggccgucg uggagaucga cgagaaggag aacccccucc gccugaucga cguggggguc      120

cggaccuucg agcgcgcgga gacccagaag ggcgaguccc uggcccucag ccggcgcucc      180

gcccggagcg cccgccggcu gacgcagcgc cggguggccc gccugaagaa ggcgaagcgg      240

cuccugaagu ccgagaacau ccugcucagc accgacgagc gccugccgca ccaggugugg      300

cagcugcggg ucgaggggcu cgaccgcaag cuggagcggc aggagugggc cgccgugcug      360

cuccaccuga ucaagcaccg cggcuaccug ucccagcgga agaacgagag caaguccgag      420

aacaaggagc ucggcgcccu gcugagcggg guggcgucca accacgagcu ccugcagcag      480

gccaccuacc gcacccccgc cgagcuggcc gucaagaagu ucgaggugga ggagggccac      540

auccggaacc agcagggggc cuacacgcac accuucagcc gccucgaccu gcuggcggag      600

auggagcucc uguucucccg gcagcagcac uucggcaacc ccuucgccag cgagaagcug      660

cucgagaacc ugaccgcccu gcucaugugg cagaagcccg cccuguccgg ggaggacauc      720

cugaagaugc ucggcaagug caccuucgag gacgaguaca aggccgcgaa gaacacguac      780

accgccgagc gcuucgugug gaucaccaag cugaacaacc ugcgcaucca ggagaacggc      840

cucgagcggg cccugagcga caacgagcgc cugaugcuca ucgagcagcc guacgagaag      900

gccaagcuga ccuacgccca gguccggucc auccugaacc ucagcgacga cgcgaucuuc      960

aagggggugc gcuacuccgg cgaggacaag aaggccaucg agacgaagac cacccugaug     1020

gagaugaagg ccuaccacca gauccggaag gugcuggagg ggaacaaccu gaaggccgag     1080

ugggucgccc ucaaggcgaa ccccacccug cuggacgaga ucggcacggc cuucagccuc     1140

uacaagaccg acgaggacau cagcgccuac cuggccggca agcuguccca gcccgugcuc     1200

aacgcgcugc uggagaaccu cagcuucgac aaguucaucc agcugucccu gaaggcccuc     1260

uacaagcugc ugccccucau gcagcagggg cugcgcuacg acgaggccug ccgggagauc     1320

uacggcgacc acuacgggaa gaagaccgag gagacccacc acuuccugcc gcagaucccc     1380

gccgacgaga uccgcaaccc cgugguccuc cggacgcuga cccaggcccg caaggugauc     1440

aacggcgugg uccggcugua cgggagcccc gcgcgcaucc acaucgagac cggccgggag     1500

gugggcaagu ccuacaagga ccgccacgag cucaagaagc ggcaggagga gaaccgcaag     1560

cagcgggaga agaccaucag cgagaucaag acgcuguucc cgaacuucuc cggggagccc     1620

aagggcaagg acauccugaa gaugcgccuc uacuaccagc agaacgccaa gugccuguac     1680

agcgggaagc cccuggagcu ccaccggcug uucgagcaga aguacgugga ggucgaccac     1740

gcccugcccc ucucccgcac cugggacgac agcuucaaca acaaggugcu gguccuggcc     1800

aacgagaacc agaacaaggg caaccucacc ccguucgagu ggcuggacgg gaagaacaac     1860

uccgagcgcu ggcggaccuu caaggcccug guggagacga gcgcguuccc cuacgccaag     1920

aagcagcgca uccucuccca gaagcuggac gagaagggcu ucaucgagcg gaaccugaac     1980

gacacccgcu acguggcccg guuccucugc aacuucaucg ccgacaacau gcaccugacc     2040

ggcgagggga agcgcaaggu cuucgccagc aacggccaga ucaccgcgcu gcuccggcgc     2100

cggugggggc uggccaaguc ccgcgaggac aacgaccggc accacgcccu ggacgccgug     2160

cucguggcgu gcagcacggu cgccaugcag cagaagauca cccgcuucgu gcgguucaag     2220

gccggcgacg uguucaccgg ggagcgcauc gaccgggaga ccggcgagau caucccccug     2280

cacuucccca cgccguggca guucuucaag caggaggucg agauccgcau cuucuccgac     2340

aaccccaagc uggagcucga gaaccggcug cccgaccgcc cccaggccaa ccacgaguuc     2400

gugcagccgc uguucgugag ccggaugccc acccgcaaga ugaccggcca ggggcacaug     2460

gagaccguca aguccgccaa gcggcucaac gagggcauca gcaugaucaa gaugccccug     2520

accaagcuga agcucaagga ccuggagcug auggugaacc gcgagcgcga gaaggaccuc     2580

uacgacgcgc ugaagacgcg gcuggaggcc uucaacgacg accccgccaa ggccuucgcc     2640

gagccguuca ugaagaaggg cggggcgauc gucaagagcg ugcgcgugga gcagguccag     2700

aaguccggcg ugcucgugcg gcaggggaac ggcguggccg acaacgccag caugguccgc     2760

guggacgugu ucaccaagga cgggaaguac uuccuggucc ccaucuacac cuggcagguc     2820

gccaagggca uccugcccaa caaggccgug auccagggga aggacgagga ggacugggag     2880

gacaucgacg acgcgaccuu ccaguucucc cuccacccga acgaccugau cagcgugaag     2940

acgaagaagg acgaguuccu gggcuacuuc aacggccuca accggcacac cgggggcauc     3000

aacauccgca cccacgaccu ggagaagucc aaggggaagc agggcaucuu cgaggggauc     3060

ggcgugaaga ucgcccugag cuucgagaag uaccagaucg acgagcucgg caagaacauc     3120

cggcugugca agcccuccaa gcgccagccc gugcgguga                            3159


<210>  1476
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1476
augaagaaca ccagccugaa cuacgugcuc ggccuggacc uggggaucuc cagcgucggc       60

ugggccgugg uggagaucga cgagaaggag aacccccucu cccugaucga cgucggggug      120

cgcaccuucg agcgggccga gacgcagaag ggcgagagcc uggcgcucgc ccgccggcug      180

gcccgcuccg cccggcgccu gacccagcgg cgcguggccc ggcucaagaa ggcgaagcgc      240

cugcucaaga gcgagaacau ccugcugucc accgacgagu gccugccgca ccaggucugg      300

cagcuccggg uggaggggcu ggagcgcaag cuggagcggc aggagugggc cgccgugcuc      360

cugcaccuga ucaagcaccg cggcuaccuc agccagcgga agaacgaguc caagagcgag      420

aacaaggagc ugggcgcccu gcucuccggg gucgcgaaca accacaagcu gcugcagcag      480

accacguacc gcacccccgc cgagcucgcc gugaagaagu ucgaggugga ggagggccac      540

auccggaacc agcaggggag cuacacccac accuucuccc gccuggaccu gcucagcgag      600

auggagcugc uguucucccg gcagcagcac uucggcaacc ccuucacgag cgagaagcuc      660

cuggagaacc ugaccgcccu ccugaugugg cagaagcccg cccuguccgg ggacgcgauc      720

cucaagaugc ugggcaagug caccuucgag aacgaguaca aggccgccaa gcacaccuac      780

agcgccgagc gcuucaucug gaucacgaag cugaacaacc uccggaucca ggagaacggc      840

cuggagcgcg cccugaacga caacgagcgc cucgcgcuga ugaagcagcc guacgagaag      900

gccaagcuga ccuacgccca gguccggucc auccucaacc ugagcgacga ggccaucuuc      960

aaggggcugc gcuacagcgg cgaggacaag aagaccuccg agaccaagac ggugcugaug     1020

gagaugaagg ccuaccacca gauccggcuc gcgcuggaga agaacgaccu gaagaccgag     1080

ugggccgagc ucaaggugaa cagcucccug cuggacgaga ucgggaccgc cuucagccuc     1140

uacaagaccg acgaggacau cuccacgugc cuggccggca agcugagcca gcccguccuc     1200

aacgcccugc uggagaaccu caacuucgac aaguucaucc agcugucccu gaaggcgcuc     1260

cacaagaucc ugccccugau gcagcagggc cuccgcuacg acgaggccug cgccgagauc     1320

uacggggacc acuacggcaa gaagaccgag gagaaccacc uccugcugcc ccagaucccg     1380

gccgacgaga uccggaaccc cguggugcug cgcacccuga cccaggcgcg gaaggucauc     1440

aacggggugg ugcgccucua cagcuccccc gcccggaucc acaucgagac gggccgcgag     1500

gucggcaaga gcuaccagga ccggcgcgag cuggagaagc ggcaggagga gaaccgcaag     1560

cagcgggaga aggccaucuc cgagaucaag acccuguucc ccaacuucag cggggagccg     1620

aaggccaagg acauccucaa gaugcgccug uacuaccagc agaacgccaa gugccuguac     1680

uccggcaagc cccucgagcu gcaccggcug cucgagcagg gguacgugga gaucgaccac     1740

gcgcugcccu ucagccgcac cugggacgac uccuucaaca acaaggugcu gguccucgcc     1800

aacgagaacc agaacaaggg caaccugacc cccuucgagu ggcuggacgg gaagaacaac     1860

agcgagcggu ggaaggccuu caaggcccuc guggagacgu ccgccuuccc guacaccaag     1920

aagcagcgca uccugagcca gaagcuggac gagaagggcu ucaucgagcg caaccucaac     1980

gacacccggu acgucgcgcg cuuccugugc aacuucaucg ccgacaacau gcaccugacc     2040

ggggagggca agcggaaggu guucgccucc aacggccaga ucacggcccu ccugcgcggg     2100

cgguggggcc uggccaagag ccgcgaggac aacgaccggc accacgcgcu cgacgccgug     2160

gucguggccu gcagcaccgu guccaugcag cagaagauca cccgcuucgu ccgguucgag     2220

gaggggaacg uguucaccgg cgagcgcauc gaccgggaga cgggggagau caucccccug     2280

cacuuccccg cccccuggaa guucuuccgc gaggaggugg agauccggau cuucagcgac     2340

aacccgaagc uggagcucga gaaccgccug aucgcgcggc cccaggccaa ccacgaguuc     2400

guccagcccc uguucguguc ccgcaugccc acccggaaga ugaccggcca gggccacaug     2460

gagaccauca agagcgccaa gcgccucaac gaggggcugu ccgugaucaa gaugcugcuc     2520

accaagcugc ggcugaagga ccucgagagc auggucaacc gcgagcggga gaaggagcug     2580

uacgaggccc ugaaggcccg ccucgaggcg uucaacgacg agccggccaa ggccuucgcc     2640

gagcccuuca ugaagaaggg cggggccauc gugaaguccg ugcgcgucga gcagguccag     2700

aaguccggcg ugcuggugcg ggacggcaac gggguggcgg acaacgccag cauggugcgc     2760

gucgacgugu ucacgaaggg cgggaaguac uuccuggugc ccaucuacac cuggcagguc     2820

gccaagggca uccuccccaa caaggccgug auccagggga agaacgagga ggacugggag     2880

gugauggacg acgccaccuu ccgguucagc cugcaccaga acgaccuggu caagcucgug     2940

accaagaaga agacgaucuu cggcuacuuc aacggccuga accgcgcgac cgggaacauc     3000

gacaucaagg agcacgaccu ggagaagucc aaggugaagg acggcaucca ccagagcauc     3060

gggaucaagc ucgcccuguc cuucgagaag uaccaggucg acgagcuggg caagaacauc     3120

cggcucugca agagcuccaa gcgccaggcc gugcgguga                            3159


<210>  1477
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1477
augaucggcg agcacgugcg cgggggcugc cuguucgacg accacuggac ccccaacugg       60

ggcgccuucc ggcuccccaa caccguccgc acguucacca aggccgagaa ccccaaggac      120

gggagcuccc uggcggagcc ccggcgccag gcccgggggc ugcgccggcg ccuccggcgc      180

aagacccagc ggcuggagga ccugcgccgg cuccuggcca aggagggcgu gcugagccuc      240

uccgaccugg agacccuguu ccgcgagacg cccgccaagg acccguacca gcuccgggcc      300

gagggccugg accgcccccu gagcuucccc gagugggugc ggguccucua ccacaucacc      360

aagcaccgcg gguuccaguc caaccggcgc aaccccgugg aggacggcca ggagcggagc      420

cgccaggagg aggaggggaa gcugcugucc ggcguggggg agaacgagcg ccuccugcgg      480

gagggcggcu accgcaccgc gggggagaug cuggcccggg acccgaaguu ccaggaccac      540

cgccggaacc gcgccggcga cuacagccac acccucuccc ggagccugcu gcucgaggag      600

gcccgccggc uguuccaguc ccagcgcacg cuggggaacc cccacgcgag cuccaaccuc      660

gaggaggccu uccugcaccu ggucgccuuc cagaaccccu ucgccagcgg cgaggacauc      720

cggaacaagg ccggccacug cucccucgag cccgaccaga uccgcgcgcc gcggcgcagc      780

gccuccgccg agaccuucau gcugcugcag aagaccggga accuccggcu gauccaccgc      840

cggaccggcg aggagcgccc ccugacggac aaggagcggg agcagaucca ccuccuggcc      900

uggaagcagg agaaggugac ccacaagacc cugcgccgcc accucgagau ccccgaggag      960

uggcuguuca ccgggcugcc cuaccaccgg agcggcgaca aggccgagga gaagcuguuc     1020

gugcaccucg cggggaucca cgagauccgc aaggcccugg acaagggccc ggaccccgcc     1080

gucugggaca cgcuccgguc ccgccgggac cugcuggaca gcaucgccga cacccucacc     1140

uucuacaaga acgaggacga gauccugccc cgccuggaga gccugggccu cucccccgag     1200

aacgcccggg cgcuggcccc gcugagcuuc uccgggaccg cccaccucag ccuguccgcc     1260

cugggcaagc uccugcccca ccuggaggag ggcaagagcu acacgcaggc ccgcgcggac     1320

gccggguacg ccgccccgcc gcccgaccgg caccccaagc ucccgccccu ggaggaggcg     1380

gacuggcgca accccguggu guuccgggcc cugacccaga cccgcaaggu cgugaacgcc     1440

cucgugcggc gcuacgggcc gcccuggugc auccaccugg agaccgcccg ggagcugucc     1500

cagcccgcca agguccgccg gcgcaucgag acggagcagc aggcgaacga gaagaagaag     1560

cagcaggccg agcgggaguu ccucgacauc gugggcaccg cccccggccc cggggaccug     1620

cugaagaugc gccucuggcg ggagcagggc ggguucugcc ccuacugcga ggaguaccug     1680

aaccccaccc gccuggccga gcccggcuac gccgagaugg accacauccu ccccuacagc     1740

cgcucccugg acaacgggug gcacaaccgg gugcuggucc acggcaagga caaccgcgac     1800

aagggcaacc ggacccccuu cgaggcguuc gggggcgaca cggcccgcug ggaccggcuc     1860

guggccuggg ugcaggccag ccaccugucc gccccgaaga agcgcaaccu gcuccgggag     1920

gacuucgggg aggaggcgga gcgcgagcug aaggaccgga accugaccga cacccgcuuc     1980

aucaccaaga cggccgccac ccuccugcgg gaccgccuga ccuuccaccc cgaggccccc     2040

aaggaccccg ucaugacccu caacggccgg cugacggcgu uccugcgcaa gcaguggggc     2100

cuccacaaga accggaagaa cggggaccug caccacgccc uggacgccgc cgugcucgcc     2160

guggcgagcc gcuccuucgu cuaccggcug agcucccaca acgccgccug gggcgagcug     2220

ccgcgcgggc gggaggccga gaacggcuuc agccuccccu accccgccuu ccgcuccgag     2280

gugcuggcgc gccugugccc cacccgggag gagauccucc ugcgccugga ccagggcggg     2340

gucggcuacg acgaggccuu ccggaacggg cuccgcccgg uguucgugag ccgggccccc     2400

ucccgccggc ugcgcggcaa ggcccacaug gagacccugc ggagccccaa guggaaggac     2460

caccccgagg ggccgcgcac cgccagccgg aucccccuca aggaccugaa ccuggagaag     2520

cucgagcgca uggucggcaa ggaccgggac cgcaagcugu acgaggcgcu gcgggagcgc     2580

cucgccgccu ucgggggcaa cggcaagaag gccuucgugg cgcccuuccg gaagcccugc     2640

cgcuccgggg agggcccgcu ggugcggagc cugcgcaucu ucgacuccgg guacagcggc     2700

gucgagcucc gggacggggg cgagguguac gccguggccg accacgaguc caugguccgc     2760

guggacgugu acgccaagaa gaaccgcuuc uaccuggucc ccguguacgu ggccgacguc     2820

gcgcggggca ucgugaagaa ccgcgccauc guggcccaca agagcgagga ggagugggac     2880

cuggucgacg gguccuucga cuuccgguuc agccucuucc ccggcgaccu gguggagauc     2940

gagaagaagg acggggccua ccugggcuac uacaaguccu gccaccgcgg ggacggccgg     3000

cuccugcugg accgccacga ccggaugccg cgcgagagcg acugcggcac cuucuacgug     3060

uccacgcgga aggacguccu cagcaugucc aaguaccagg uggacccccu gggggagauc     3120

cgccuggugg gcagcgagaa gccgcccuuc guccucuga                            3159


<210>  1478
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1478
augaagcgca acuacauccu gggccucgac aucgggauca ccagcguggg cuacgggauc       60

aucgacuacg agacccggga cgugaucgac gccggcgugc gccuguucaa ggaggccaac      120

guggagaaca acgaggggcg gcgcuccaag cggggcgcgc gccggcugaa gcgccggcgc      180

cggcaccgca uccagcgggu caagaagcuc cuguucgacu acaaccugcu cacggaccac      240

agcgagcugu ccggcaucaa ccccuacgag gcccgcguga aggggcugag ccagaagcuc      300

uccgaggagg aguucagcgc cgcccugcug caccucgcca agcggcgcgg cgugcacaac      360

gucaacgagg uggaggagga caccgggaac gagcugucca ccaaggagca gaucagccgg      420

aacuccaagg cgcuggagga gaaguacgug gccgagcucc agcuggagcg ccugaagaag      480

gacggcgagg uccgggggag caucaaccgc uucaagaccu ccgacuacgu gaaggaggcc      540

aagcagcucc ugaaggugca gaaggccuac caccagcugg accagagcuu caucgacacg      600

uacaucgacc uccuggagac ccggcgcacc uacuacgagg gcccgggcga gggguccccc      660

uucggcugga aggacaucaa ggagugguac gagaugcuga uggggcacug caccuacuuc      720

cccgaggagc uccgcagcgu caaguacgcg uacaacgccg accuguacaa cgcccugaac      780

gaccucaaca accuggugau cacgcgggac gagaacgaga agcuggagua cuacgagaag      840

uuccagauca ucgagaacgu guucaagcag aagaagaagc ccacccucaa gcagaucgcc      900

aaggagaucc uggucaacga ggaggacauc aagggcuacc gcgugaccuc caccggcaag      960

ccggaguuca cgaaccugaa gguguaccac gacaucaagg acaucaccgc ccggaaggag     1020

aucaucgaga acgcggagcu ccuggaccag aucgccaaga uccugaccau cuaccagagc     1080

uccgaggaca uccaggagga gcucaccaac cugaacagcg agcugacgca ggaggagauc     1140

gagcagaucu ccaaccucaa gggguacacc ggcacccaca accugagccu gaaggccauc     1200

aaccucaucc uggacgagcu guggcacacc aacgacaacc agaucgccau cuucaaccgc     1260

cucaagcugg uccccaagaa gguggaccug agccagcaga aggagauccc cacgacccuc     1320

guggacgacu ucauccuguc ccccgucgug aagcggagcu ucauccaguc caucaaggug     1380

aucaacgcca ucaucaagaa guacgggcug ccgaacgaca ucaucaucga gcuggcgcgc     1440

gagaagaaca gcaaggacgc ccagaagaug aucaacgaga ugcagaagcg gaaccgccag     1500

accaacgagc ggaucgagga gaucauccgc accacgggca aggagaacgc caaguaccuc     1560

aucgagaaga ucaagcugca cgacaugcag gaggggaagu gccuguacuc ccucgaggcc     1620

aucccccugg aggaccugcu caacaacccc uucaacuacg aggucgacca caucaucccc     1680

cggagcgugu ccuucgacaa cagcuucaac aacaaggucc uggugaagca ggaggagaac     1740

uccaagaagg gcaaccgcac cccguuccag uaccugagcu ccagcgacuc caagaucagc     1800

uacgagaccu ucaagaagca cauccucaac cuggccaagg gcaaggggcg gaucuccaag     1860

accaagaagg aguaccugcu cgaggagcgc gacaucaacc gguucagcgu gcagaaggac     1920

uucaucaacc gcaaccuggu cgacacgcgg uacgcgaccc gcggccugau gaaccuccug     1980

cgguccuacu uccgcgugaa caaccuggac gugaagguca agagcaucaa cgggggcuuc     2040

accuccuucc uccgccggaa guggaaguuc aagaaggagc gcaacaaggg guacaagcac     2100

cacgccgagg acgcccugau caucgccaac gccgacuuca ucuucaagga guggaagaag     2160

cuggacaagg cgaagaaggu gauggagaac cagauguucg aggagaagca ggccgagagc     2220

augcccgaga ucgagaccga gcaggaguac aaggagaucu ucaucacgcc ccaccagauc     2280

aagcacauca aggacuucaa ggacuacaag uacagccacc ggguggacaa gaagcccaac     2340

cgcgagcuca ucaacgacac ccuguacucc acccggaagg acgacaaggg caacacccug     2400

aucgucaaca accucaacgg ccuguacgac aaggacaacg acaagcugaa gaagcucauc     2460

aacaagagcc cggagaagcu gcugauguac caccacgacc cccagacgua ccagaagcuc     2520

aagcugauca uggagcagua cggggacgag aagaaccccc uguacaagua cuacgaggag     2580

accggcaacu accucaccaa guacuccaag aaggacaacg ggcccgugau caagaagauc     2640

aaguacuacg gcaacaagcu gaacgcccac cuggacauca ccgacgacua cccgaacagc     2700

cgcaacaagg uggucaagcu cucccugaag cccuaccggu ucgacgugua ccuggacaac     2760

gggguguaca aguucgucac cgugaagaac cucgacguga ucaagaagga gaacuacuac     2820

gaggucaaca gcaagugcua cgaggaggcc aagaagcuga agaagaucuc caaccaggcg     2880

gaguucaucg ccagcuucua caacaacgac cugaucaaga ucaacggcga gcucuaccgc     2940

gugaucggcg ugaacaacga ccugcugaac cggaucgagg ucaacaugau cgacaucacg     3000

uaccgcgagu accucgagaa caugaacgac aagcggccgc cccgcaucau caagaccauc     3060

gccuccaaga cccagagcau caagaaguac uccaccgaca uccuggggaa ccuguacgag     3120

gucaagagca agaagcaccc ccagaucauc aagaagggcu ga                        3162


<210>  1479
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1479
augaagcgca acuacauccu gggccucgac aucgggauca ccagcguggg cuacgggauc       60

aucgacuacg agacccggaa cgucaucgac gcgggcgugc gccuguucaa ggaggccaac      120

guggagaaca acgaggggcg gcgcuccaag cggggcacgc gccggcugaa gcgccggcgc      180

cggcaccgcc uccagcgggu caagaagcug cuguucgacc acaacuuccu caccgacaac      240

agcgagcugu ccggcaucag ccccuacgag gcgcgcguga aggggcugaa ccagaagauc      300

uccgaggagg aguucagcgu ggcccuccug caccuggcca agcggcgcgg cguccacaac      360

gugaacgagg uggaggagga caccgggaac gagcucucca ccaaggagca gaucagccgg      420

aacuccaagg cccuggagga gaaguacguc gccgagcugc agcucgagcg ccugaaggug      480

gacggcgagg ugcgcggggc ggucaaccgg uucaagacga gcgacuacgu gaaggaggcc      540

cagcagaucc ugaaggugca gaaggccuac caccaguucg accaguccuu caucgacacc      600

uacaucgacc uccuggagac ccggcgcacc uacuacgagg gcccgggcga ggggagcccc      660

uucggcugga aggacaucaa ggagugguac gagaugcuga uggggcagug cacguacuuc      720

cccgaggagc uccgcuccgu caaguacgcc uacaacgcgg accuguacaa cgcccugaac      780

gaccucaaca accuggugau cgcccgggac gagaacgaga agcuggagua cuacgagaag      840

uuccagauca ucgagaacgu guucaagcag aagaagaagc ccacccucaa gcagaucgcc      900

aaggagaucc uggucaacga ggaggacauc aagggcuacc gcgugaccag caccggcaag      960

ccggaguuca cgaacuucaa gguguaccac gacaucaagu ccaucaccag ccggaaggag     1020

auccuggaga acgccgagcu ccugaaccag aucgcgaaga uccugaccau cuaccagucc     1080

agcgaggaca uccaggggga gcucgcccgc cuguacuccg agcugaccca ggaggagauc     1140

gagcagauca gcaagcucgc cggcuacacg gggacccacc ggcugagccu gaaggccauc     1200

aaccucauca ucgacgagcu guggcacacc uccgacaacc agauggagau cuucaaccgc     1260

cugaagcucg uccccaagaa gguggaccug agccagcaga aggagauccc caccacgcug     1320

guggacgacu ucauccucuc ccccgucgug aagcggagcu ucauccaguc caucaagguc     1380

aucaacgcca ucaucaagaa guacggcuuc ccgaacgaca ucaucaucga gcuggcgcgc     1440

gagaagaaca gcaaggacgc ccagaaguuc aucaacgaga ugcagaagcg gaaccgccag     1500

accaacgagc ggaucgagga gaucauccgc accaccggga aggagaacgc caaguaccug     1560

aucgagaaga ucaagcucca cgacaugcag gagggcaagu gccuguacuc ccuggaggcc     1620

aucccccugg aggaccuccu gaacaacccc uucaacuacg agguggacca caucaucccc     1680

cggagcgugu ccuucgacaa cagcuucaac aacaaggucc uggugaagca ggaggagaac     1740

uccaagaagg gcaaccgcac gccguuccag uacuucagcu ccagcgacuc caagaucagc     1800

uacgagaucu ucaagaagca cauccucaac cuggccaagg ggaagggccg gaucuccaag     1860

aagaagaagg aguaccugcu cgaggagcgc gacaucaacc gguucagcgu gcagaaguac     1920

uucaucaacc gcaaccuggu cgacacccgg uacgcgaccc gcgagcugau gaaccuccug     1980

cgcuccuacu uccgggugaa cgaccuggac aucaagguca agagcaucaa cgggggcuuc     2040

accagcuucc uccgcaagaa guggaaguuc aagaaggagc ggaacaaggg guacaagcac     2100

cacgccgagg acgcccugau caucgccaac gccgacuuca ucuucaagga guggaagaag     2160

cuggacaaga cgaacaaggu gauggaggac cagaucgugg aggagcagca ggcggagucc     2220

augcccgaga ucgagaccga gcaggaguac aaggagaucu ucaucacgcc ccaccagauc     2280

caggacauca aggacuucaa ggacuacaag uacagccacc gcguggacaa gaagcccaac     2340

cgggagcucg ucaacgacac ccuguacucc acccgcaagg acgacaaggg caacacccug     2400

aucgugaaca accucaaggg ccuguacgac aaggacaacg acaagcugaa gaagcucauc     2460

aacaagagcc cggagaagcu gcugauguac caccacgacc cccagaccua ccagaagcuc     2520

aaggugauca uggagcagua cuccaacgag aagaaccccc uguacaagua ccacgaggag     2580

accgggaacu accugacgaa guacagcaag aaggacaacg gccccgucau caagaagauc     2640

aaguacuacg ggaacaagcu caacguguac cuggacauca cccgggacua ccugaagucc     2700

cgcaacaaga ucgugaagcu cagccugaag ccguaccggu ucgacgucua ccuggacaac     2760

ggcguguaca aguucgugac cgucaagaac cucgacauca ucaagaagga ggacuacuac     2820

gagguggacu ccgacagcua cgaggaggcc aagaucguga agaaggucuc cgagcaggcc     2880

caguucgugg ccagcuucua caacaacgac cugaucaagg uggacgggga gcuguuccgc     2940

gucaucggcg ugaacaacga ccuccugaac cggaucgagg ugaacauggu cgacaucacc     3000

uacaaggagu accuggagaa caucaacgac aagaagucca gccgcaucau caagaccauc     3060

uccagcaaga cgcaguccau caagaaguac agcaccgaca uccucggcaa ccuguacgag     3120

gccaagagca agcagcggcc ccagaugacc augaaggggu ga                        3162


<210>  1480
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1480
augaagcgca acuacauccu gggccucgac aucgggauca ccagcguggg cuacgggauc       60

aucgacuacg agacccggga cgucaucgac gccggcgugc gccuguucaa ggaggccaac      120

guggagaaca acgaggggcg gcgcuccaag cggggcgcgg gccgccugaa gcggcgccgg      180

cgccaccggc uccagcgcgu caagaagcug cuguucgacu acaaccuccu gacggaccac      240

agcgagcugu ccgggaucaa ccccuacgag gcccggguga agggccucag ccagaagcug      300

uccgaggagg aguucagcgc cgcccugcuc caccuggcca agcgccgcgg ggugcacaac      360

gucaacgagg uggaggagga caccggcaac gagcugucca ccaaggagca gaucagccgg      420

aacuccaagg cgcucgagga gaaguacgug gccgagcugc agcuggagcg gcucaagacc      480

gacggggagg uccgcggcag caucaaccgg uucaagacgu ccgacuacgu gaaggaggcc      540

aagcagcugc ugaaggugca gaaggaguac caccagcucg accagagcuu caucgacacc      600

uacaucgacc ugcuggagac ccgccggacc uacuacgagg gcccggggga gggcuccccc      660

uucgggugga aggacaucaa ggagugguac gagaugcuca ugggccacug cacguacuuc      720

cccgaggagc ugcgcagcgu caaguacgcc uacaacgcgg accuguacaa cgcccucaac      780

gaccugaaca accuggugau cgcccgcgac gagaacgaga agcucgagua cuacgagaag      840

uuccagauca ucgagaacgu guucaagcag aagaagaagc ccacccugaa gcagaucgag      900

aaggagaucc uggucaacga ggaggacauc aagggcuacc gggugaccuc caccgggaag      960

ccggaguuca gcaaccucaa gguguaccac gacaucaagg acaucacggc ccgcaaggag     1020

aucaucgaga acgccgagcu gcuggaccag aucgcggaga uccucaccau cuaccagucc     1080

agcgaggaca uccaggagga gcuggucaag cugaacuccg agcucaccca ggaggagauc     1140

gagcagauca gcaaccugaa gggcuacacc gggacgcaca accugagccu caaggccauc     1200

aaccugaucc uggacgagcu cuggcacacc aacgacaacc agauggccau cuucaaccgg     1260

cugaagcugg ugcccaagaa gguggaccuc ucccagcaga aggagauccc caccacccug     1320

gucgacgacu ucauccugag ccccguggug aagcgcuccu ucauccagag caucaagguc     1380

aucaacgcca ucaucaagaa guacggccug ccgaacgaca ucaucaucga gcucgcccgg     1440

gagaagaacu ccaaggacgc gcagaagaug aucaacgaga ugcagaagcg caaccggcag     1500

acgaacgagc gcaucgagga gaucauccgg accaccggga aggagaacgc caaguaccug     1560

aucgagaaga ucaagcugca cgacaugcag gagggcaagu gccucuacag ccuggaggcc     1620

aucccccugg aggaccuccu gaacaacccc uucaacuacg agguggacca caucaucccc     1680

cgcuccguca gcuucgacaa cuccuucaac aacaaggugc uggugaagca ggaggagaac     1740

agcaagaagg gcaaccggac cccguuccag uaccucucca gcuccgacag caagaucucc     1800

uacgagacgu ucaagaagca cauccugaac cuggccaagg ggaagggccg caucagcaag     1860

accaagaagg aguaccuccu ggaggagcgg gacaucaacc gcuucuccgu ccagaaggac     1920

uucaucaacc ggaaccuggu ggacacccgc uacgccaccc gggagcucau gaaccugcug     1980

cgcagcuacu uccgggugaa caaccucgac gucaagguga aguccaucaa cgggggcuuc     2040

acgagcuucc ugcgccgcaa guggaaguuc aagaaggagc ggaacaaggg guacaagcac     2100

cacgcggagg acgcccugau caucgccaac gccgacuuca ucuucaagga guggaagaag     2160

cucgacaagg ccaagaaggu gauggagaac cagaccgucg aggagaagca ggcggagagc     2220

auguccgaga ucgagaccga gcaggaguac aaggagaucu ucaucacgcc ccaccagauc     2280

cagcacauca aggacuucaa ggacuacaag uacagccacc gcguggacaa gaagcccaac     2340

cgggagcuga ucaacgacac ccuguacucc acccgcaagg acgacaaggg caacacccuc     2400

aucgugaaca accugaacgg ccuguaccac aaggacaacg acaagcucaa gaagcugaug     2460

aacaagagcc ccgagaagcu gcucauguac caccacgacc cgcagaccua ccagaagcug     2520

aagcugauca uggagcagua cggggacgag aagaaccccc ucuacaagua cuacgaggag     2580

acgggcaacu accugaccaa guacuccaag aaggacaacg ggcccgucau caagaagauc     2640

aaguacuacg gcaacaagcu gaacgcccac cucgacauca ccgacgacua ccccaacagc     2700

cggaacaagg uggugaagcu gucccugaag ccguaccgcu ucgacgucua ccucgacaac     2760

gggguguaca aguucgugac cgucaagaac cugaacguga ucaagaagga gaacuacuac     2820

gaggugaaca gcaagugcua cgagaaggcc aagaagcuga agaagaucuc cgaccaggcc     2880

gaguucaucg cgagcuucua caacaacgac cucaucaaga ucgacggcga gcuguaccgg     2940

gucaucggcg ugaacaccga ccugaucaac cgcaucgagg ugaacauggu cgacaucacg     3000

uaccgggagu accucgagaa caugaacgac aagcgcagcc cccggaucuu caagaccauc     3060

gccuccaaga cccaguccau caagaaguac agcaccgaca uccuggggac gcuguacgag     3120

gugaacucca agaagcaccc ccagaugauc augaagggcu ga                        3162


<210>  1481
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1481
augagcggca ugguguucgg gaucgaccug ggcaucgccu ccugcgggug ggccguccuc       60

cgccagcccc agcgggacgg cgacccgggg gagaucgugg accugggcag cuggauguuc      120

gacgugcccg agaccgacaa ggagcgcacc cccacgaacc agguccggcg cggcaaccgg      180

cugcuccgcc gggugauccg ccggcgcgcg cagcggaugg uggagauccg ccggcuguuc      240

cacgaccacg ggcugcucgc cggccacgcc cccgaggccc ugaagcgcgc cgggcuggac      300

ccgugggacc uccgggcgcg cucccuggac aagguccugg agcccgccgc ccucgccgug      360

gcgcugggcc acaucgccaa gcggcgcggg uucaagagcg ccgcccggcg caaggaggcc      420

aacaccgcgg gcgacgacca gaagaugcug aaggcccucg aggccaccca cgagcggcug      480

ggccgcuacc gcaccaucgg ggagauguuc gcccgggacc ccgacuucga gucccgcaag      540

cggaaccgcg acggcauguu cgaccggacg caggggcgcg acgaccugcu ccacgaggug      600

ggcgagaucu ucaaggccca gcggcgccug ggcagcgcgc uggccaccgc cgagcucgag      660

caggccuuca ccgccaucgc guuccggcag cugcccaucc aggacuccga gcgccugguc      720

gggcucugcc aguucgagcc gaaggagaag cgggccgccc gcuucgcccc cagcuucgag      780

cgguuccgcc ugcugcagcg gcucaccaac cugcgcgugg ugacggucga gggcgagcgg      840

ccccugaccg ccgacgagau cgcggccgcc gccgcggacc ucgggcgcac cgccaagcug      900

uccgugaagg aggugcggaa gcgcaucggc cuggccgccg accaccgcuu cgccgcguuc      960

aaggccgacg aggaggaccg ggacaucauc gcccgcaccg gggaggcccu ccacggcacg     1020

uaccggcugc gcaaggcccu gggcgagggg cucugggcgg agaugcugcc cggccagcug     1080

gacgccaucg cccacgcccu cagcuucuuc gagacccagg acgucauccu gaaggagcug     1140

gacaagcucg accugccggc cggggugcgg gacgcgaucg ccaccggccu ggacgccggg     1200

gccuucgcgc gcuucaaggg caccggccac aucuccgccc gggccgcccg cgcccuccug     1260

ccccaccugg aggcggggcu ccgguacgac caggccugca cgaaggccgg cuacgaccac     1320

gccgccagcc gcugggcgaa gcgggagcag gucgccgaca aggccgccuu caaccgccug     1380

gugaccgaca ugggggccga gaucgcgaac cccaucgccc ggaagucccu gaccgaggcc     1440

cugaagcagc ucugggccau gcgcaaccgg uggggccugc ccgacgcgau ccacaucgag     1500

cuggcccgcg acguggggaa cagccucgag aagcggcgcg agaucgagcg ggccaucgag     1560

aagaacaccg ccgcccgcga gcgggagcgc ggcgaggcgc gcgaccugcu gggcaucgac     1620

gacguguccg gggacacgcu ccugcgguac cgccugugga aggagcaggc cggccggugc     1680

ccguacaccg acgcccccau cccgcccggg gccaucaucg ccaccgacaa cagcuuccag     1740

gucgaccaca uccucccgug gucccgcuuc ggcgacgaca gcuucgcgaa caaggugcug     1800

ugcgccaccg ccgccaacca gcggaagaag ggccagacgc ccugcgagug gaucaccgcc     1860

gcgcaggggg aggagggcug ggccaccuuc guggcccgca ucgaggggaa cgccgccuuc     1920

cggggcccca agaagcgcaa cuacguccug aagaacgcga aggaggccga ggagcgguuc     1980

cgcgcccgga accucaacga cacccgcuac gccgcgcggc ugcuggccga ggccgugaag     2040

cuguucuacc ccgaggggga gcgccaggac aagggcggcg uccggcgcgu guucacgcgg     2100

cccgggggcc ucaccgccgc ccugcgccac gcguggggcg uggaggcccu caagaagcgg     2160

gacgggaagc gcgucgacga cgcccgccac cacgcccugg acgcccuggu gguggcggcc     2220

aucggggagg gcgaggucca gcggcucacc cgcuccuacc aggaguggga gcagcagggc     2280

cuggcccggc cccugcgccg gguggacccg cccuggggcg acuuccacag cuuccgccgg     2340

gaggugaagg acgccuacga cgggaucuuc gucgcccgcc ccgagcggcg ccgggcgcgc     2400

ggggagggcc acgccgccac cauccgccag gugcgggagc gggacggggc cgcggugguc     2460

uucgagcgca aggccaucgc cgaccucagc gagaagcggc uggccgacau caaggacccc     2520

gagcgcaacc aggccaucgu ggaggcgauc cggcagugga ucguggacgg ccgccccgcc     2580

gaccggcugc cgcgcucccc cgccggcgac gagauccgca agguccggcu ccgcacgaag     2640

gggaagcccg ccguccaggu gcggggcggg gccgcggacc gcggcgagau cgugcgggug     2700

gacguguuca ccaagcccaa caagaagggg aagaacgagu ucuaccuggu cccgaucuac     2760

ccccaccagg ugaugaacaa ggccgagugg cccacgcccc cgaugcgcgc cguggucgcc     2820

uacaaggacg agagcgagug gacccugcuc gacgagaacu ucggcuuccu guucucccug     2880

uucccccgga gcuacgugga ggugaccaag cccggcgggg agguccucuc cggcuacuuc     2940

caggggaugg accgcagcac cggcgccauc ucccuguuca accaccggga cagccgcucc     3000

cugaccgacg acagcggcaa cuccacccgg gggaucggcg cgaagacccu ccugacgaug     3060

aagaaguaca gcguggaccg cuucgggaag cgggccgagg ugaaguccga gguccgcacc     3120

uggcacggcg uggccugcac cuaccccacc ccgcccgggu ga                        3162


<210>  1482
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1482
augaagcuga cgccccucaa cuacauccug ggccuggacc ucgggaucgc cagcgugggc       60

ugggccgucg uggagaucga cgagcaggag aacccgcugg ggcugaucga cgugggcguc      120

cgcaccuucg accgggcgga gguggccaag accggggagu cccucgcccu ggcccgccgg      180

cuggcccgca gcucccggcg ccucgugaag cggcgcgcgg accggaucaa gaaggucaag      240

cgccugcuca agagcgagaa cauccuccug uccgccgacg agcagcugcc caacgacgug      300

uggcagcucc gggugaaggg ccuggaccag aagcuggagc gccaggagug ggccgccguc      360

cuccugcacc uccugaagca ccggggcuac cugagccagc gcaagaacga guccaagagc      420

gagaacaagg agcugggggc gcuccggucc ggcguggccg ccaaccacca gauccugcag      480

agcgccgagu accgcacccc cgccgagauc gcggugaaga aguuccacgu cgaggagggg      540

cacauccgga accagcgcgg cgccuacacc cacaccuucu cccggcugga ccuccuggcc      600

gagaucgagc ugcuguucac gcgccagacc gagcugggga acccccacac cagcgccaag      660

cugcucgaga accugaccgc ccugcucaug uggcagaagc cggcgcuggc cggcgaggcc      720

auccugaaga ugcucggcaa gugcacguuc gagcccaccg aguacaaggc cgccaagaac      780

uccuacagcg cggagcgguu cguguggcug accaagcuga acaaccuccg cauccuggag      840

cagggggccg agcgcgcccu gaccgacaac gagcgguucg cccuccugga ccagcccuac      900

gagaaggcca aguucacgua cgcgcaggcc cgcaccaugc uggcccuccc cgacgaggcc      960

aucuucaagg gcgugcggua ccagggggag gacaagaagg cggucgagac caagaccauc     1020

cugauggaga ugaaggccua ccaccagauc cgcaaggccc uggagaacgc cgaccugaag     1080

gccgagugga acgagcucaa gaacaacucc gagcugcugg acgacaucgg cacggcguuc     1140

agccuccaca agaccgacga ggacaucugc cgguaccugg acggcaagcu guccgagagc     1200

auccucaacg cccugcugga gaaccucaac uucgacaagu ucauccagcu gucccugaag     1260

gcccuccagc agauccugcc gcugaugcuc caggggcagc gcuacgacga ggccgugagc     1320

gccaucuacg gcgaccacua cgggaagaag uccgcggaga ucaaccggcu gcugcccacc     1380

auccccgccg acgagauccg caaccccgug guccuccgga cccugacgca ggcccgcaag     1440

gugaucaacg ccgugguccg gcuguacggc agcccggccc gcauccacau cgagaccggg     1500

cgggaggugg gcaagagcua ccaggaccgc aagaagcucg agaagcagca ggaggacaac     1560

cggaagcacc gcgaguccgc ggugcagaag uucaaggagu acuuccccaa cuucgucggc     1620

gagcccaagg ggaaggacau ccugaagaug cggcuguacg agcuccagca ggccaagugc     1680

cuguacagcg gcaagucccu ggagcuccac cgccugcugg agaaggggua cguggagguc     1740

gaccacgccc uccccuucag ccggaccugg gacgacuccu ucaacaacaa ggugcuggug     1800

cuggccaacg agaaccagaa caagggcaac cucaccccgu acgaguggcu ggacgggaag     1860

aacaacagcg agcccuggca gaacuucguc gcgcgcgugc agacguccgg cuucagccac     1920

accaagaagc agcgcauccu gucccacaag cucgacgaga agggcuucau cgagcggaac     1980

cugaacgaca cccgcuacgu gguccgguuc cugugcaacu ucaucgccga caacaugcuc     2040

cugaccggga agggcaagcg caagguguuc gccagcaacg ggcagaucac ggcccugcuc     2100

cggggccgcu gggggcugca gaaggugcgg gacgacaacg accgccacca cgcccuggac     2160

gcggucgugg uggccugcuc caccgucgcc augcagcaga agaucacccg guucgugcgc     2220

uacgaggagg gcaacguguu cagcggcgag cggaucgacc gcgagaccgg ggagaucauc     2280

ccccuccacu uccccucccc gugggccuuc uuccgggaga acgucgagau ccgcaucuuc     2340

agcgagaacc ccaagcugga gcuggagaac cggcuccccg acuaccccca guacaaccac     2400

gaguucgugc agccgcuguu cgugucccgc augcccacgc ggaagaugac cggccagggg     2460

cacauggaga ccgucaagag cgccaagcgc cuggacgagg gccucuccgu gcugaaggug     2520

ccccugaccc agcucaagcu gaacgaccug gagcggaugg ucaaccgcga gcgggagaug     2580

gcgcucuacg agagccugaa ggugcgccug gagcaguucg acaacgaccc cgccaaggcc     2640

uucgccgagc cguucuacaa gaagggcggg gcccucguga aggcgguccg cguggagcag     2700

acgcagaagu ccggcgugcu gguccgggac gggaacggcg uggccgacaa cgccagcaug     2760

gugcgcgucg acguguucac caaggccggg aaguacuucc uggugcccau cuacaccugg     2820

caggucgcga agggcauccu ccccaacaag gccgugaccg ccaacgugga cgagaucgac     2880

uggcuggaga uggacgagag cuaccaguuc aucuucacca uguacccgaa cgaccugguc     2940

aaggugaagc ucaagaagga ggaguucuuc ggcuacuacg ggggccugga ccgggccacg     3000

ggggccaucg ugaucaagga gcacgaccug gagaagucca agggccgcca ggggaucuac     3060

cggaucggcg ucaagcucgc gcugagcuuc gagaaguacc agguggacga gcugggcaag     3120

aacauccgcc ccugccggcc caccaagcgc cagcacgugc gguga                     3165


<210>  1483
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1483
augcgcaucc ugggcgugga ccucgggguc gccagcgugg gcugggcccu ggugaacgac       60

aagaacaaca agaucaucga cuccgggguc cggaucuuca cccagagcac caaccgcgag      120

ggcaagacgc uggcggccgu gcggcgcucc uuccgcgggg gccggcgggu gcucagccgc      180

cggaagcagc gccugaacca gcugaagaag cuguucaucg cccacaagcu gcuguccaag      240

agcgaguuca agcaccucuu caacuccaag aaccggcugg acaucuggca gcugcgcgcc      300

aagggccucg agcagcagcu gaacaacaag gagugggccc ggguccugua ccacaucgcg      360

aagcgccggg ccuaccagag caaccgcaag uccgaggaga gcgggaacac cgacaagaag      420

aaggugcucu ccggcaucag caagaacgag aagaagauga acgagcuggg guacaagacc      480

aucggcgaga ugcuguacac cgagaaccgg uaccccgaca accgcauccg gcgcaacaag      540

aaggaccagu acaagcacuc caucagccgg gaccuccugc gcaaggagau cgacgugcug      600

uucgaguccc agcgcgacuu cgggaaccag uucgccacgg acggccucaa ggagcacuac      660

aaggacaucg ccuucaccca gcggccgauc aaguucaagg cggaccuggu cggcaagugc      720

accuucgaga cccaggagua ccgcggggcc aagaacugcu ucagcgugga gaaguccacg      780

cugcucagca agaucaacaa caccgaccug aucgacaagu ccaccggcga gaucaagcgg      840

cuguucgaga ucagcgaguu cgacaagcuc cuggacaucu ucuacagcau caaggaggug      900

aaguacacca cgcugcgcaa gcagcucaag aucaccgacg ccuucaaguu caagcagcug      960

gacuaccacc agaacuucga guacaagacc aacaucaagg ccaagaucga ggacuucuac     1020

aagcuggagg acuggcucac ccccgagcag aagguccugc uggagaagca gguccucgac     1080

gacaagaagg agaaguucaa gugcuacaag uacuccagca uccgguccau ccugagccug     1140

uccgagagcc agaaguucaa cggggugaag uacgacacga agguggagaa caccguguuc     1200

ggcaagcuca acggcuacca cuccaucaag aaggagcuga acaaggaggu guucgaccuc     1260

cucuucaucg acaaggacaa guucaacaag aucgccgaga uccugagcua ccagaaggac     1320

gacaaguccg cgacccagga ccugaagaag caggucuuca acgacuucaa cauggacaag     1380

accaucguga acgaggccau caacagccuc cugaacaucu ccuucagcgg guucaacucc     1440

cugagccuca aggccaucaa caagcugcug cccuaccugu gccagggccu caaguaccac     1500

gacgccgugg cccacuccga guacaagcac cacagccagu ucaaggcggc caacccccag     1560

caguaccugc gcccgcugaa caaggaggag aacuaccaga ucacgucccc caccgucaag     1620

cggguguuca gccaguuccg caaggugcuc aacgccguca uccggaagca cggguccuuc     1680

gacgccaugc acaucgagau ggcccgcgag cugaagaaca gcaagaagau caaguucgag     1740

auccagaccg gccagaagga guacgcgcag gagaaggagg ccauccgggu gaaguuccug     1800

gagaucuuca aguacgaggg gagcggccgc gaccuccuga agcugcggcu cuacgagcag     1860

cagcacggca agugcaucua cuccaagaag gagaucgaga ucaacaagcu gcuggaggac     1920

ggguacgucg agaucgacca cauccucccc uggagccgca ccuucgacaa cucccugaac     1980

aacaaggccc ugugccucag cgccgagaac cagaacaagg ccaaccagac gcccuucgag     2040

uaccugacca acuccgacaa gaacagcaag gacuccaagg aguggcagga guacaagaac     2100

uacgugagcu ccuucgcggg caucaagcag gccaagaagc acaagcugcu caacaccacc     2160

cugccgcccc ggcgcgggaa cgacuucagc gacgacauca agaaccccga guccggcuuc     2220

cuggcccgga accucaacga cacggccuac gcgagccgcu ucaucaagaa cuucgugcag     2280

aaccaccucc aguucuccca gaacgacacc aucaagcaga aggucaaggu gcggagcggg     2340

gcccugacca accagcugcg cuacaacugg ggcaucgagg agaagaaccg cgacaacaac     2400

cugcaccacg ccgaggacgc caucauccug gccuucgcga cccagggcga ggugcagcgg     2460

auguccacga ucagcgccaa gcgcgaggac uucaaguacc agaccgccaa ggagcggaag     2520

aucaaguuca cgcccccguu cuccgacuuc aagagcgucc ucgacgagag cgugggggac     2580

aucuucgugu ccuucgcgcc ccgccggaag aucagcggcg ccgcccacaa ggccaccauc     2640

aagcccaagg acaccggggg caaguacaag uuccccguca acaacgggau ggccgagaac     2700

ggcgugaucc agcgcgugga cgucuucaag aacgccaaga acaaguacca guucaucgug     2760

uucuacccgg ccgacuucua caaggacgac uuccccgagc uggaccugaa gggcaacgag     2820

aucgacaaga acgugcaguu ccucuucucc cuguucaagg acgaccugau cgaguucaag     2880

accaagaaga ccaagaccaa ggacagcaag caccuccugg cguacuucaa guacauccgg     2940

uccgacggga agaucgccuu ccagcagaac uacaaggccg agaucgagcg caaggagagc     3000

gacaacaaga agggcuacaa ggacauccug cucgccaccg gguccggccu ggcgagccug     3060

aagaaguacc aggucuccgu gcucggcgac aucgccgagg ugaagaacga gaagcggcug     3120

ccccugauca agcagaugua caagagcaag cccaagaaga aguga                     3165


<210>  1484
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1484
augaaccaga aguucauccu gggccucgac aucgggauca ccagcguggg cuacgggcug       60

aucgacuacg agaccaagaa caucaucgac gccggcgucc gccuguuccc cgaggccaac      120

guggagaaca acgaggggcg gcgcuccaag cggggcagcc gccggcucaa gcgccggcgc      180

auccaccggc uggagcgcgu gaagaagcug cucgaggacu acaaccugcu ggaccagucc      240

cagaucccgc agagcacgaa ccccuacgcg auccggguca agggccucuc cgaggcccug      300

agcaaggacg agcuggugau cgcccuccug cacaucgcca agcgccgggg gauccacaag      360

aucgacguga ucgacuccaa cgacgacguc ggcaacgagc ugagcaccaa ggagcagcuc      420

aacaagaacu ccaagcugcu gaaggacaag uucgugugcc agauccagcu cgagcgcaug      480

aacgaggggc agguccgggg cgagaagaac cgcuucaaga ccgccgacau caucaaggag      540

aucauccagc ugcugaacgu gcagaagaac uuccaccagc ucgacgagaa cuucaucaac      600

aaguacaucg agcuggugga gaugcggcgc gaguacuucg aggggcccgg caagggcagc      660

cccuacgggu gggagggcga cccgaaggcg ugguacgaga cccugauggg gcacugcacg      720

uacuuccccg acgagcuccg guccgugaag uacgccuaca gcgccgaccu guucaacgcc      780

cugaacgacc ucaacaaccu ggucauccag cgcgacggcc uguccaagcu cgaguaccac      840

gagaaguacc acaucaucga gaacguguuc aagcagaaga agaagcccac ccugaagcag      900

aucgcgaacg agaucaacgu gaaccccgag gacaucaagg gcuaccggau caccaagagc      960

gggaagccgc aguucaccga guucaagcug uaccacgacc ucaaguccgu ccuguucgac     1020

cagagcaucc uggagaacga ggacgugcuc gaccagaucg ccgagauccu gacgaucuac     1080

caggacaagg acuccaucaa gagcaagcug accgagcucg acauccugcu gaacgaggag     1140

gacaaggaga acaucgccca gcucaccggc uacaccggga cgcaccgccu gucccugaag     1200

ugcauccgcc ucgugcugga ggagcagugg uacagcagcc ggaaccagau ggagaucuuc     1260

acccaccuga acaucaagcc caagaagauc aaccucaccg ccgccaacaa gauccccaag     1320

gcgaugaucg acgaguucau ccuguccccc gucgugaagc gcaccuucgg ccaggccauc     1380

aaccugauca acaagaucau cgagaaguac ggggugccgg aggacaucau caucgagcug     1440

gcccgggaga acaacagcaa ggacaagcag aaguucauca acgagaugca gaagaagaac     1500

gagaacacgc gcaagcggau caacgagauc aucggcaagu acggcaacca gaacgccaag     1560

cgccucgucg agaagauccg gcugcacgac gagcaggagg ggaagugccu guacucccuc     1620

gagagcaucc cccuggagga ccugcucaac aaccccaacc acuacgaggu ggaccacauc     1680

aucccccgcu ccgugagcuu cgacaacucc uaccacaaca agguccuggu gaagcagagc     1740

gagaacucca agaaguccaa ccugaccccg uaccaguacu ucaacagcgg caagagcaag     1800

cucuccuaca accaguucaa gcagcacauc cugaaccuga gcaaguccca ggaccggauc     1860

agcaagaaga agaaggagua ccuccuggag gagcgcgaca ucaacaaguu cgagguccag     1920

aaggaguuca ucaaccggaa ccugguggac acccgcuacg ccacccggga gcucacgaac     1980

uaccugaagg cguacuucuc cgccaacaac augaacguga aggucaagac caucaacggg     2040

agcuucaccg acuaccugcg caaggugugg aaguucaaga aggagcggaa ccacggcuac     2100

aagcaccacg ccgaggacgc ccucaucauc gccaacgcgg acuuccuguu caaggagaac     2160

aagaagcuga aggccgugaa cuccguccuc gagaagcccg agaucgagag caagcagcug     2220

gacauccagg uggacagcga ggacaacuac uccgagaugu ucaucauccc caagcagguc     2280

caggacauca aggacuuccg caacuucaag uacagccacc ggguggacaa gaagcccaac     2340

cgccagcuga ucaacgacac ccucuacucc acgcggaaga aggacaacag caccuacauc     2400

gugcagacca ucaaggacau cuacgccaag gacaacacca cgcugaagaa gcaguucgac     2460

aaguccccgg agaaguuccu gauguaccag cacgaccccc gcaccuucga gaagcucgag     2520

gugaucauga agcaguacgc caacgagaag aacccccugg ccaaguacca cgaggagacc     2580

ggggaguacc ugaccaagua cagcaagaag aacaacggcc ccaucgucaa gucccucaag     2640

uacaucggca acaagcuggg gagccaccug gacgugacgc accaguucaa guccagcacc     2700

aagaagcucg ugaagcuguc caucaagccg uaccgcuucg acgucuaccu gaccgacaag     2760

ggcuacaagu ucaucaccau cagcuaccuc gacgugcuga agaaggacaa cuacuacuac     2820

auccccgagc agaaguacga caagcugaag cucgggaagg cgaucgacaa gaacgccaag     2880

uucaucgccu ccuucuacaa gaacgaccug aucaagcugg acggcgagau cuacaagauc     2940

aucgggguga acagcgacac gcggaacaug aucgagcucg accugcccga cauccgcuac     3000

aaggaguacu gcgagcugaa caacaucaag ggcgagcccc ggaucaagaa gaccaucggc     3060

aagaagguca acuccaucga gaagcucacc accgacgugc uggggaacgu guucaccaac     3120

acgcaguaca ccaagccgca gcuccuguuc aagcgcggca acuga                     3165


<210>  1485
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1485
augaagcuga cgccccucaa cuacauccug ggccuggacc ucgggaucgc cagcgugggc       60

ugggccgucg uggagaucga cgagcaggag aacccgcugg ggcugaucga cgugggcguc      120

cgcaccuucg accgggcgga ggugcccaag accggggagu cccucgcccu ggcccgccgg      180

cuggcccgca gcgcccggcg ccucgugaag cggcgcgcgg accggaucaa gaaggccaag      240

cgccugcuga aggccgagaa cauccuccug uccgccgacg agcaccuccc caacgacguc      300

uggcagcugc gggugaaggg ccuggaccag aagcuggagc gccaggagug ggcggccgug      360

cuccugcacc uccugaagca ccggggcuac cugagccagc gcaagaacga guccaagagc      420

gagaacaagg agcugggggc ccuccugucc ggcgucgaga ccaaccacca gauccugcag      480

agcgccgagu accggacccc cgccgagauc gcggugaaga aguuccacgu ggaggacggg      540

cacauccgca accagcgggg cgccuacacc cacacguucu cccgccucga ccugcuggcc      600

gagauggagc uccuguucca gcggcagacc gaccugggga acccgcacac cagcgccaag      660

cuccuggaga accugaccgc ccuccugaug uggcagaagc ccgcgcuggc cggcgaggcc      720

auccucaaga ugcugggcaa gugcacguuc gagcccaccg aguacaaggc cgccaagaac      780

uccuacagcg cggagcgcuu cgucuggcug accaagcuca acaaccugcg gauccuggag      840

cagggggccg agcgcgcccu caccgacaac gagcgcuucg cccugcugga ccagcccuac      900

gagaaggcca aguucacgua cgcgcaggcc cggaccaugc ucgcccugcc ggacgaggcc      960

aucuucaagg gcgugcgcua ccagggggag gacaagaagg cgguggagac caagaccauc     1020

cugauggaga ugaaggccua ccaccagauc cggaaggccc uggagaacgc cgaccucaag     1080

gccgagugga acgagcugaa gaacaacucc gagcugcucg acgacaucgg cacggcguuc     1140

agccugcaca agaccgacga ggacaucugc cgcuaccugg acggcaagcu cuccgagagc     1200

auccugaacg cccugcucga gaaccugaac uucgacaagu ucauccagcu gucccucaag     1260

gcccugcagc agauccugcc ccucaugcug caggggcagc gguacgacga ggccgucagc     1320

gccaucuacg gcgaccacua cgggaagaag uccgcggaga ucaaccgccu gcuccccacc     1380

auccccgccg acgagauccg gaacccggug gugcugcgca cccugacgca ggcccggaag     1440

gucaucaacg ccguggugcg ccucuacggc agccccgccc ggauccacau cgagaccggg     1500

cgcgaggucg gcaagagcua ccaggaccgg aagaagcugg agaagcagca ggaggacaac     1560

cgcaagcagc gggaguccgc ggugaagaag uucaaggagu acuuccccaa cuucgugggc     1620

gagcccaagg ggaaggacau ccugaagaug cgccucuacg agcugcagca ggccaagugc     1680

cuguacagcg gcaagucccu cgagcugcac cggcugcucg agaaggggua cgucgaggug     1740

gaccacgccc ugccguucag ccgcaccugg gacgacuccu ucaacaacaa gguccuggug     1800

cucgccaacg agaaccagaa caagggcaac cugacccccu acgaguggcu ggacgggaag     1860

aacaacagcg agcacuggca gaacuucgug gcgcgggucc agacguccgg cuucagccac     1920

accaagaagc agcgcauccu cucccacaag cuggacgaga agggcuucau cgagcgcaac     1980

cugaacgaca cccgguacgu ggcccgcuuc cucugcaacu ucaucgccga caacaugcug     2040

cugaccggga agggcaagcg gaagguguuc gccagcaacg ggcagaucac ggcccuccug     2100

cgcggccggu gggggcugca gaagguccgc gacgacaacg accggcacca cgcgcucgac     2160

gccguggugg ucgccugcuc caccguggug augcagcaga agaucacccg cuucguccgg     2220

uacgaggagg gcaacguguu cagcggcgag cgcaucgacc gggagaccgg ggagaucauc     2280

ccccugcacu uccccucccc gugggccuuc uuccgcgaga acguggagau ccggaucuuc     2340

agcgagaacc ccaagcugga gcucgagaac cgccugcccg acuaccccca guacaaccac     2400

gaguucgucc agccgcuguu cgugucccgg augcccacgc gcaagaugac cggccagggg     2460

cacauggaga ccgucaagag cgccaagcgg cucgacgagg gccuguccgu gcugaaggug     2520

ccccucaccc agcugaagcu gagcgaccuc gagcgcaugg ugaaccggga gcgcgagguc     2580

acgcuguacg agucccugaa ggcgcggcuc gagcaguucg gcaacgaccc cgccaaggcc     2640

uucgccgagc cguuccacaa gaagggcggg gccgugguga aggcgguccg cguggagcag     2700

acccagaagu ccggggugcu gguccgcgac ggcaacgggg uggccgacaa cgccagcaug     2760

gugcgggucg acguguucac caagggcggc aaguacuucc uggugcccau cuacaccugg     2820

caggucgcca aggggauccu ccccaacaag gcggugaccg ccaacgugga cgagaucgac     2880

uggcuggaga uggacgagag cuaccaguuc aucuucacga uguacccgaa cgaccugguc     2940

aaggugaagc ucaagaagga ggaguucuuc ggcuacuacg ggggccugga ccgcgccacc     3000

ggggccaucg ugaucaagga gcacgaccug gagaagucca agggcaagca gggcaucuac     3060

cggaucgggg ucaagcucgc ccugagcuuc gagaaguacc agguggacga gcugggcaag     3120

aacauccgcc ccugccggcc caccaagcgc cagcacgugc gguga                     3165


<210>  1486
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1486
augaaggaga aguacauccu gggccucgac cuggggauca ccagcguggg cuacgggauc       60

aucaacuucg agaccaagaa gaucaucgac gccggcgucc gccuguuccc cgaggccaac      120

guggacaaca acgaggggcg gcgcuccaag cggggcagcc gccggcucaa gcgccggcgc      180

auccaccggc uggagcgcgu gaagcugcuc cugacggagu acgaccugau caacaaggag      240

cagaucccga ccuccaacaa ccccuaccag auccggguca agggccucag cgagauccug      300

uccaaggacg agcuggccau cgcccuccug caccuggcca agcgccgggg gauccacaac      360

aucaacguga gcuccgagga cgaggacgcc agcaacgagc ucuccaccaa ggagcagauc      420

aaccgcaaca acaagcugcu gaaggacaag uacgugugcg agguccagcu ccagcggcug      480

aaggagggcc agauccgcgg ggagaagaac cgguucaaga ccacggacau ccugaaggag      540

aucgaccagc uccugaaggu gcagaaggac uaccacaacc uggacaucga cuucaucaac      600

caguacaagg agaucgugga gacccgccgg gaguacuucg agggccccgg gcagggcagc      660

cccuucggcu ggaacgggga ccucaagaag ugguacgaga ugcugauggg ccacugcacc      720

uacuucccgc aggagcugcg cuccgucaag uacgccuaca gcgcggaccu cuucaacgcc      780

cugaacgacc ugaacaaccu caucauccag cgggacaacu ccgagaagcu ggaguaccac      840

gagaaguacc acaucaucga gaacguguuc aagcagaaga agaagcccac ccugaagcag      900

aucgccaagg agaucggggu gaaccccgag gacaucaagg gcuaccgcau caccaagagc      960

ggcacgcccc aguucaccga guucaagcuc uaccacgacc ugaaguccau cgucuucgac     1020

aagagcaucc uggagaacga ggccauccuc gaccagaucg cggagauccu gaccaucuac     1080

caggacgagc aguccaucaa ggaggagcug aacaagcucc cggagauccu gaacgagcag     1140

gacaaggccg agaucgccaa gcugaucggg uacaacggca cgcaccgccu cagccugaag     1200

ugcauccacc ugaucaacga ggagcucugg cagaccuccc ggaaccagau ggagaucuuc     1260

aacuaccuga acaucaagcc caacaaggug gaccugagcg agcagaacaa gauccccaag     1320

gacaugguga acgacuucau ccucagcccc gucgugaagc gcaccuucau ccaguccauc     1380

aacgugauca acaaggucau cgagaaguac gggaucccgg aggacaucau caucgagcug     1440

gcccgggaga acaacagcga cgaccgcaag aaguucauca acaaccugca gaagaagaac     1500

gaggccaccc ggaagcgcau caacgagauc aucggccaga cggggaacca gaacgcgaag     1560

cggaucgugg agaagauccg ccugcacgac cagcaggagg gcaagugccu cuacucccug     1620

gagagcaucg cccugaugga ccuccugaac aacccccaga acuacgaggu ggaccacauc     1680

aucccccggu ccgucgccuu cgacaacagc auccacaaca aggugcuggu gaagcagauc     1740

gagaacucca agaagggcaa ccgcaccccc uaccaguacc ucaacagcuc cgacgccaag     1800

cugagcuaca accaguucaa gcagcacauc cugaaccucu ccaagagcaa ggaccggauc     1860

uccaagaaga agaaggacua ccugcuggag gagcgcgaca ucaacaaguu cgagguccag     1920

aaggaguuca ucaaccggaa ccucguggac acccgcuacg ccacccggga gcugacgagc     1980

uaccugaagg cguacuucuc cgccaacaac auggacguca aggugaagac caucaacggg     2040

agcuucacca accaccuccg caaggugugg cgguucgaca aguaccgcaa ccacggcuac     2100

aagcaccacg ccgaggacgc ccugaucauc gccaacgcgg acuuccuguu caaggagaac     2160

aagaagcucc agaacaccaa caagauccug gagaagccga cgaucgagaa caacaccaag     2220

aaggucaccg uggagaagga ggaggacuac aacaacgugu ucgagacccc caagcugguc     2280

gaggacauca agcaguaccg ggacuacaag uucucccacc gcguggacaa gaagcccaac     2340

cgccagcuca ucaacgacac gcuguacagc acccggauga aggacgagca cgacuacauc     2400

gugcagacca ucaccgacau cuacgggaag gacaacacga accugaagaa gcaguucaac     2460

aagaaccccg agaaguuccu cauguaccag aacgacccga agaccuucga gaagcugucc     2520

aucaucauga agcaguacag cgacgagaag aacccccugg ccaaguacua cgaggagacc     2580

ggcgaguacc ucaccaagua cagcaagaag aacaacgggc ccaucgucaa gaagaucaag     2640

cugcugggca acaagguggg caaccaccuc gacgugacga acaaguacga gaacuccacc     2700

aagaagcugg ucaagcugag caucaagaac uaccgcuucg acguguaccu caccgagaag     2760

ggguacaagu ucgugaccau cgccuaccug aacgucuuca agaaggacaa cuacuacuac     2820

auccccaagg acaaguacca ggagcugaag gagaagaaga agaucaagga caccgaccag     2880

uucaucgccu ccuucuacaa gaacgaccuc aucaagcuga acggcgaccu guacaagauc     2940

aucgggguga acagcgacga ccggaacauc aucgagcucg acuacuacga caucaaguac     3000

aaggacuacu gcgagaucaa caacaucaag ggcgagccgc gcaucaagaa gacgaucggg     3060

aagaagaccg aguccaucga gaaguucacc accgacgugc ugggcaaccu guaccuccac     3120

agcacggaga aggcgcccca gcugaucuuc aagcggggcc uguga                     3165


<210>  1487
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1487
auggagaaca agaaccugaa cuacauccuc ggccuggacc uggggaucgc cagcgugggc       60

ugggccgucg uggagaucga cgagaaggag aacccccucc gccugaucga cguggggguc      120

cggaccuucg agcgcgcgga ggugccgaag accggcgagu cccuggcccu cagccggcgc      180

cuggcccggu ccgcccgccg gcugacgcag cgccgggugg cccgccucaa gaaggcgaag      240

cggcugcuca agagcgagaa cauccugcug uccaccgacg agcgccugcc ccaccagguc      300

uggcagcucc ggguggaggg gcuggaccac aagcuggagc gccaggagug ggccgccgug      360

cuccugcacc ugaucaagca ccggggcuac cucagccagc gcaagaacga guccaagagc      420

gagaacaagg agcugggcgc ccugcucucc ggggucgaca acaaccacaa gcugcugcag      480

caggcgaccu accggagccc cgccgagcuc gccgugaaga aguucgaggu ggaggagggc      540

cacauccgca accagcaggg ggccuacacc cacacguucu cccggcugga ccugcucgcc      600

gagauggagc ugcuguucag ccgccagcag cacuucggca accccuucgc guccgagaag      660

cuccuggaga accugaccgc ccuccugaug uggcagaagc cggcccugag cggggaggcc      720

auccucaaga ugcugggcaa gugcaccuuc gaggacgagu acaaggccgc gaagaacacc      780

uacuccgccg agcgguucgu cuggaucacg aagcugaaca accuccgcau ccaggagaac      840

ggccuggagc gcgcccugaa cgacaacgag cggcucgccc ugauggagca gcccuacgac      900

aagaaccgcc uguucuacag ccaggugcgg uccauccuca agcugagcga cgaggccauc      960

uucaaggggc ugcgcuacuc cggcgaggac aagaaggcga ucgagaccaa ggccgugcug     1020

auggagauga aggccuacca ccagauccgg aagguccucg aggggaacaa ccugaaggcc     1080

gagugggccg agcugaaggc gaaccccacc cuccuggacg agaucggcac cgccuucagc     1140

cuguacaaga cggacgagga caucagcgcc uaccucgccg gcaagcuguc ccagcccgug     1200

cugaacgcgc uccuggagaa ccugagcuuc gacaaguuca uccagcucuc ccugaaggcc     1260

cuguacaagc uccugccgcu gaugcagcag gggcuccgcu acgacgaggc cugccgggag     1320

aucuacggcg accacuacgg gaagaagacc gaggagaacc accacuuccu gccccagauc     1380

cccgccgacg agauccgcaa ccccgugguc cugcggaccc ucacccaggc ccgcaaggug     1440

aucaacggcg ugguccggcu guacgggagc ccggcgcgca uccacaucga gacgggccgg     1500

gaggugggca aguccuacaa ggaccgccgg gagcuggaga agcgccagga ggagaaccgg     1560

aagcagcgcg agaacgccau caaggaguuc aaggaguacu ucccccacuu cgccggggag     1620

cccaaggcca aggacauccu caagaugcgg cuguacaagc agcagaacgc caagugccug     1680

uacagcggca agcccaucga gcuccaccgc cugcuggaga agggguacgu ggaggucgac     1740

cacgcgcucc cguucucccg caccugggac gacagcuuca acaacaaggu gcugguccug     1800

gccaacgaga accagaacaa gggcaaccuc acccccuucg aguggcugga cgggaagcac     1860

aacuccgagc gguggcgcgc cuucaaggcc cugguggaga ccagcgccuu ccccuacgcg     1920

aagaagcagc ggauccucuc ccagaagcug gacgagaagg gcuucaucga gcgcaaccug     1980

aacgacacgc gguacguggc ccgcuuccuc ugcaacuuca ucgccgacaa caugcaccug     2040

accggcgagg ggaagcggaa ggucuucgcc agcaacggcc agaucaccgc gcugcuccgc     2100

ucccgguggg ggcuggccaa gagccgcgag gacaacgacc ggcaccacgc ccuggacgcc     2160

gugguggucg ccugcuccac cguggcgaug cagcagaaga ucacgcgcuu cgugcgguuc     2220

gaggccggcg acgucuucac cggggagcgc aucgaccggg agaccggcga gaucaucccc     2280

cuccacuucc cgacccccug gcaguucuuc aagcaggagg uggagauccg caucuucagc     2340

gacaacccca agcuggagcu ggagaaccgg cuccccgacc gcccgcaggc caaccacgag     2400

uucgugcagc cccuguucgu cucccggaug cccacgcgca agaugaccgg ccaggggcac     2460

auggagaccg ucaagagcgc caagcggcug aacgagggca ucuccgugau caagaugccc     2520

cucaccaagc ugaagcugaa ggaccucgag cugaugguga accgcgagcg cgagaaggac     2580

cuguacgaca cgcucaaggc ccggcuggag gcguucaacg acgacccggc caaggccuuc     2640

gccgagcccu ucaucaagaa gggcggggcc aucgucaaga gcgugcgcgu ggagcagauc     2700

cagaaguccg gcgugcuggu gcgggagggg aacggcgucg cggacaacgc cagcauggug     2760

cgcguggacg ucuucaccaa gggcgggaag uacuuccucg ugcccaucua caccuggcag     2820

gucgccaagg ggauccugcc gaacaaggcc gcgacccagu acaaggacga ggaggacugg     2880

gaggugaugg acaacagcgc cacguucaag uucucccugc accccaacga ccucgugaag     2940

cuggugacca agaagaagac cauccugggc uacuucaacg gccucaaccg ggccaccggg     3000

aacaucgaca ucaaggagca cgaccuggac aagagcaagg gcaagcaggg gaucuucgag     3060

ggcgucggga ucaagcuggc ccucuccuuc gagaaguacc agguggacga gcugggcaag     3120

aacauccgcc ugugcaagcc cagcaagcgg cagcccgugc gcuga                     3165


<210>  1488
<211>  3168
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1488
augaacaacu acauccuggg ccucgacauc gggaucacca gcgugggcua cgggaucguc       60

gacuccgaca cccgcgagau caaggacgcc ggcgugcggc uguuccccga ggccaacgug      120

gacaacaacg aggggcgccg gagcaagcgc ggcgcccggc gccugaagcg gcgccggauc      180

caccgccucg accgggucaa gcaccuccug gccgaguaca accugcugga ccugacgaac      240

aucccgaagu ccaccaaccc cuaccagauc cgcgugaagg gccucaacga gaagcugagc      300

aaggacgagc uggugaucgc ccuccugcac aucgccaagc ggcgcgggau ccacaacguc      360

aacgugauga uggacgacaa cgacuccggc aacgagcuga gcaccaagga ccagcucaag      420

aagaacgcca aggcgcuguc cgacaaguac gugugcgagc ugcagcucga gcgguucgag      480

caggacuaca agguccgcgg ggagaagaac cgguucaaga ccgaggacuu cgugcgcgag      540

gcccggaagc ugcuggagac gcagagcaag uucuucgaga ucgaccagac cuucaucaug      600

cgcuacaucg accucgugga gacccggcgc gaguacuucg agggccccgg gaagggcucc      660

cccuucggcu gggaggggaa caucaagaag ugguucgagc agaugauggg ccacugcacc      720

uacuucccgg aggagcugcg cagcgucaag uacgccuacu ccgccgagcu guucaacgcg      780

cucaacgacc ugaacaaccu ggugaucacg cgggacgagg aggccaagcu caacuacggg      840

gagaaguucc agaucaucga gaacguguuc aagcagaaga agacccccaa ccugaagcag      900

aucgccaagg agaucggcgu cagcgagacc gacaucaagg gcuaccgcgu gaacaagucc      960

gggaagcccg aguucaccca guucaagcug uaccacgacc ucaagaacau cuucgaggac     1020

agcaaguacc ugaacgacgu gcagcugaug gacaacaucg ccgagaucau cacgaucuac     1080

caggaccccg aguccaucau caaggagcuc aaccugcugc cggagcuccu gagcgagaag     1140

gagaaggaga agaucuccgc ccugagcggc uacgcgggga cccaccggcu cucccugaag     1200

ugcaucaacc ugcuccugga cgaccugugg gagagcagcc ucaaccagau ggagcuguuc     1260

accaagcuga accucaagcc caagaagauc gaccuguccc agcagcacaa gauccccauc     1320

aagcuggucg acgacuucau ccugagcccc guggugaagc gcgccuucau ccaguccauc     1380

caggucguga acgccaucau cgacaaguac ggccucccgg aggacaucau caucgagcug     1440

gcccgggaga acaacagcga cgaccgccgg aaguuccuga accagcucca gaagcagaac     1500

gccgagaccc gcaagcaggu cgagaaggug cugcgggagu acgggaacga caacgcgaag     1560

cgcaucgugc agaagaucaa gcugcacaac augcaggagg gcaagugccu cuacucccug     1620

aaggacaucc cccuggagga ccuccugaag aaccccaacc acuacgaggu cgaccacauc     1680

aucccccgga gcguggccuu cgacaacucc augcacaaca aggugcuggu ccgcgccgag     1740

gagaacagca agaagggcaa ccggacgccg uaccaguacc ucaacuccag cgaguccagc     1800

cuguccuaca acgaguucaa gcagcacauc cugaaccuca gcaagaccaa ggaccgcauc     1860

accaagaaga agcgggagua ccugcuggag gagcgcgaca ucaacaagua cgacgugcag     1920

aaggaguuca ucaaccggaa ccucguggac acccgcuacg ccacgcggga gcugaccucc     1980

cugcucaagg ccuacuucag cgcgaacaac cuggacguca aggugaagac caucaacggg     2040

uccuucacca acuaccugcg caaggugugg aaguucgaca aggaccgcaa caagggcuac     2100

aagcaccacg ccgaggacgc ccucaucauc gccaacgccg acuuccuguu caagcacaac     2160

aagaagcugc ggaacaucaa caagguccuc gacgcgccca gcaaggagau cgacaagaag     2220

cgcgugacgg ugcaguccga ggacgaguac aaccagaugu ucgaggacac ccagaaggcc     2280

caggccauca agaaguucga gauccggaag uucagccacc gcgucgacaa gaagcccaac     2340

cggcagcuga ucaaggacac ccuguacagc acccgcaaca ucgacgggau cgaguacgug     2400

guggagucca ucaaggacau cuacagcguc aacaacgaca aggugaagac gaaguucaag     2460

aaggaccccc accggcuccu gauguaccgc aacgacccgc agaccuucga gaaguucgag     2520

aagguguuca agcaguacga guccgagaag aaccccuucg ccaaguacua cgaggagacc     2580

ggcgagaaga uccggaaguu cagcaagacc gggcagggcc ccuacaucaa caagaucaag     2640

uaccugcgcg agcggcucgg ccgccacugc gacgucacga acaaguacau caacucccgg     2700

aacaagaucg ugcagcugaa gaucuacagc uaccgcuucg acaucuacca guacgggaac     2760

aacuacaaga ugaucaccau cuccuacauc gaccuggagc agaaguccaa cuacuacuac     2820

aucagccggg agaaguacga gcagaagaag aaggacaagc agaucgacga cagcuacaag     2880

uucaucggcu ccuucuacaa gaacgacauc aucaacuaca acggggagau guaccgcgug     2940

aucggcguca acgacagcga gaagaacaag auccagcucg acaugaucga caucuccauc     3000

aaggacuaca uggagcugaa caacaucaag aagaccgggg ugaucuacaa gaccaucggc     3060

aagagcacga cccacaucga gaaguacacc accgacaucc ugggcaaccu cuacaaggcg     3120

gcgcccccga agaagcccca gcugaucuuc aagcggggga uggaguga                  3168


<210>  1489
<211>  3168
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1489
augaccccca ucagcgaguc ccuccuguuc ggccuggacg uggggaucag cuccugcggc       60

ugggccgucc ugaagcacag cgacaccacg gggcacaucg aggcccucgg cuccuggugc      120

uucaacgccc cggagaccaa gaccgagcgc acccccacga accagauccg gcgcagcucc      180

cggcugcugc gccggguggu gcgccggcgc cggaaccgca ugaccgagau ccggcgccuc      240

uucgcccggc acgggcugau cgacaacgac cgccccgacg cccugcggca gcccgccauc      300

gacccguggg agaugcgcgg ccagggccuc gaccggcagc ugacccccga ggaguucgcc      360

gucgcgcugg cccacaucgc caagcaccgc ggguucaaga gcuccgccaa gcgggucaag      420

agcaacgcgu cccccgacga cagcaagaug cucgccgccc uggagaagac caaggagcug      480

uccgcccagu accgcacgau cggcgagaug uucgcccggg acagcgcgua cgugcagcgc      540

cgccggaacc gcgacggccu cuucgaccgg accaucuccc gcgacgaccu gaucgacgag      600

gccaagaucc uguucgaccg gcagcgcaag cucgggaacc ccguggccag caccgagauc      660

gaggaggccu ucacccggau cgccuucugg cagaacccga ugcaggacuc cggggugcug      720

guggcgagcu gccccuucga gcccaaggag aagcgcucca gccgguucag ccccuccuuc      780

gagcgcuucc ggcugcucug ccgccugauc aaccugcggg ucacggacgg cccggaggag      840

cgcccccuca ccgaggagga gcugcggcug gccuacgagc acgccggcaa gacccgcaag      900

cucaccgccg ccgcgauccg gaagcugauc gggcugcgcg acgaccagca cuucacgacc      960

aucccgcccg agcaggagaa gcaggacauc accagccgga ccggcgagac guucgccggg     1020

accgccaccu uccgcuccgu gcucggcgag agccuguggc gccagcugcu cccccacccc     1080

gaguggcugg acaacgccgc cuggaucauc uccuuccacg agcugaccga gacgaucauc     1140

aagaagcucg gcgagaucgg gcugcccgug gaggcguacg agcugaucug cgacgcccuc     1200

aagagcgaca agucccccuu cgccaaguuc aagggcgcca gcucccugag cgacaaggcg     1260

guccgggccc ugaucccgua ccuccagcag gggcugaccu acgacaaggc cugccagaag     1320

gccggcuacg accacgccgc gucccgcuuc agcgggcggg ccaccaucga caccaaggcc     1380

aaguucaacg cccugauggc cgaggugcgc gacuccaucg cgaaccccau cgcccggaag     1440

gcccucagcg agggcaugaa gcagcugugg gccaugcgca accggugggg ccugcccggg     1500

gccaucugca ucgagcucgc gcgcgacgug ggcaacuccc uggagaagcg gaaggagauc     1560

gagcgcaacc ugaagacgac cgccaagcag cgggcccgcg agcgggagga ggccugcgag     1620

cugcucaacc ugaaggaggu cagcuccgac auccugcucc gcuaccggcu guggaaggag     1680

cagggcgggc gcugcugcua cagcgggaag uccaucaccc ccgacaugcu gauggcgcag     1740

gacaaccgga uccagaucga ccacauccuc ccguggagcc gcuucggcga cgacagcuac     1800

uccaacaagg gccugugccu ggccaaggag aaccaggcca agaagaacca gacccccuac     1860

gaguggaucc acgggcaggg cacgaccgcc cagaaggagc agugggacaa guucguggcc     1920

gacguggaga gccggaagaa caccaagggg cucaagaagc gcaacuuccu gcugaagggc     1980

uccaaggaga ucgaggagcg guuccgcagc cgcaaccuca acgacacccg guacgcggcc     2040

cgccugaugg ucgaggccgc ccggcugcuc uaccccgccg ggcagcgcgg cgagaagggc     2100

gggacgcggc gcguguucac ccggcccggc gcgcugaccg ccgcccugcg ccacgccugg     2160

ggcguggagu cccucaagaa gaucgacggg gagcggcugc acgacaaccg ccaccacgcc     2220

cuggacgcgg ccaucgucgc cgccgugagc gaggcggaga uccagaagcu caccaagucc     2280

uuccaggagu gcgagcagaa gggccugagc cggccgaugc gcgacguggc cccgcccugg     2340

gacggguucc gggaccagcu gaaggagaag uacgagacca ucuucgucgc ccgccccgag     2400

cggcagcgcg cccggggcca ggggcacgag gccacgaucc gccaggugcg ggaggaggag     2460

accggccccg ucaucuacga gcgcaaggcg guguccgccc ucacccuggc cgaccuggcc     2520

aagaucaagg accccgaccg caacgccggg cucaucgcga gccugcggca guggaucgag     2580

gccggcaagc ccgccgacaa gccgccccgc acccccugcc accaguccgc cgccggccac     2640

gagauccgga aggugcgccu ggcgacgauc aucacccccg ccgucccggu gcggggcggg     2700

auggccaagc gcggggagau gacccgggug gacaucuuca aggucaucgg caagaagggg     2760

aagcccgagu gguacaugau ccccaucuac ccccaccaga ucaugaaccg cagccagugg     2820

ccggccccgc ccaacaaggc cgugguggcg ggcaaguccg aggccgacug gcccaucaug     2880

ggcgccgagg cccaguucca guucagccuc uaccccugcu ccuacguccg gcuguucaag     2940

gaggggaccg ugaucgaggg cuacuucugc gggugccacc gcagccgggc guacaucacg     3000

cuggcccucu ccaccggcgc cgcgccccug cagaagggga ucggcaucaa gacccaggag     3060

cacauccaga aguucagcgu gaaccgcuuc ggcgagcuga gccccgucaa gcaggagacc     3120

cggacguggc acgggguggc cugcaccucc cccgaccagc acgacuga                  3168


<210>  1490
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1490
augaaggccc ugaacuacau ccucggccug gaccugggga ucgccagcgu gggcugggcg       60

gucguggaga ucgaccagaa cgagcucccc auccgccuga ucgaccuggg ggugcggacc      120

uucgagcgcg ccgagguccc gaagaccggc gacucccucg cccuggcccg gcgccuggcc      180

cggagcuccc gccggcucgu gcgccggcgc gcggugcggc ugcugaaggc caagcgccuc      240

cugaagcagg aggggauccu gcaggccgac gacuuccaca gcucccaccu caagccccug      300

cccaacaacc ccugggagcu gcgggucaag ggccucaacc accuccugga ccgcaaggag      360

ugggccgcgg ugcuccugca ccuccugaag caccggggcu accugagcca gcgcaagaac      420

gaguccaaga cggacaacaa ggagcugggg gcccuccuga agggcgugag cgagaaccgg      480

cagcugcucc aggcccaggc cuucuccacc ccggccgaga ucgcgaucaa gaaguuccag      540

caggaggagg ggcacauccg caaccagcgg ggcgccuaca gccacaccuu cgaccgcaac      600

gaccugcugg ccgagcuccg gcugcuguuc cagaagcagc gcgaguucaa caaccccuac      660

accucccaga gccucgagca ggccaucgcc gagcugcuga uguggcagaa gcccgcgcuc      720

uccggggagg ccauccugaa gaugcugggc aagugcacgu ucgagcccag cgaguacaag      780

gccccgaaga acuccuacag cgccgaguac uucgucuggc ucaccaagcu gaacaaccug      840

cgcauccucg agaacggcgc cgaccggggg cugaccgcgg aggagcgcca gcugcucauc      900

aaccagcccu ucgagaaggc caaguucacc uacgcccagg cccggaagcu gcuggagcug      960

uccgaccucg ccaucuucaa gggcgugcgc uacgggaagg acaacgcgga gagcgccacg     1020

cugauggaga ugaaggccug gcacgccauc cggaaggcgc uggaguccaa cggccucaag     1080

accgagugga acggccugaa gaccaagccc gagcugcucg acaagaucgg gaccgccuuc     1140

agcaucuaca agacggacga ggacaucagc caguaccuga acggcgagcu gucccccgcc     1200

gugcucaacg cccugcugga gaaccucagc uucgacaagu ucauccgccu gucccugcag     1260

agccucgcca agauccugcc gcugauggag caggggcucc gguacgacga ggcgugcaag     1320

caggucuacg gcgaccacua cgggaagaag gagcagcugg agcugcgcuu ccuccccaag     1380

auccccgugg aggacauccg gaaccccgug guccugcgca cccuguccca ggcccggaag     1440

gugaucaacg gcgugguccg ccucuacggc agcccggccc ggauccacau cgagaccggg     1500

cgcgaggugg gcaaguccuu cagcgagcgg cgcgacaucu ccaagcggca ggaggagaac     1560

cgcaacgagc ggcagcgcgc cguggagaag uucaaggagu ucuuccccca cuucgucggg     1620

gagcccaagg ccaaggacau ccugaagaug cgccuguacg agcagcagca cggcaagugc     1680

cucuacagcg ggaaggagcu ggaccugaac cggcuccugg agaagggcua cguggagauc     1740

gaccacgcgc ugcccuucuc ccgcaccugg gacgacagcu ucaacaacaa ggugcucguc     1800

cuggccuccg agaaccagaa caagggcaac cagacgccgu acgaguggcu gaacgggaag     1860

gagaacgccg agcgguggaa gcuguuccag gcccugguga acggcagccg cuuccccuac     1920

uccaagaagc agcggauccu gaccaaguac cucgacgagg acggguuccg cgagcggaac     1980

cugaacgaca cccgcuacgu cgcccgguuc cugugcaacu ucaucgagga caacaugcag     2040

cucgugggcc aggggaagaa gcgcguguuc gcgagcaacg gccagaucac cgcccugcug     2100

cggcgcuccu ggggcaucca gaagguccgg gaggacaacg accgccacca cgccguggac     2160

gccguggucg uggcgugcag cacgcgggcc cuccagaaga agaucaccga cuacgugcgc     2220

uacaaggaga ugaacgucuu caccggggag aagaucgacc aggagaccgg cgagaucauc     2280

aaguuccacu uccccgagcc cuggaaguuc uuccggcagg agguggagau ccgcguguuc     2340

uccgagacgc cggccgagga cgucgugauc gcccugcccg accggcccca ggccaaccac     2400

gaguucaucc agccccuguu cgugagccgc gcgccgaccc ggaagaugac cgggcagggc     2460

cacauggaga ccaucaagag cgccaagcgc cucgccgagg gccuguccgu cagccgcgug     2520

ccccugacgc agcucaagcu gaaggaccug gagaacaugg ugaaccggga gcgcgagaag     2580

gcccucuacg aggcccugaa gguccggcug gagaaguuca acaacgaccc cgcgaaggcc     2640

uucgccgacc cguucuacaa gaagggcggg cagcagguca aggccgugcg cguggagcag     2700

guccagaagu ccggggugcu cgugcggcag gagaacggcg uggccgacaa cgcgagcaug     2760

gugcgcgucg acguguucau caagggcggg aaguacuucc uggugcccau cuacaccugg     2820

caggucgcca agggcauccu gcccaaccgg gccgccaccc aggggaagga ggaggaguac     2880

ugggacauca uggacgagac cgcgaucuuc caguucuccc ucuaccagaa cgaccugauc     2940

aagcugguga cgaagaagaa gaccaucuuc ggcuacuuca acgggcucaa ccgcgccacc     3000

agcaacauca acaucaagga gcacgaccug gacaagucca agggcaagga cgggaucuac     3060

cuggaggugg gcgucaagcu cgcccugagc cuggagaagu accaggugga cgagcucggc     3120

aagaacaucc agcccugccg guccaugaag aagcgcccgc ccgugcggug a              3171


<210>  1491
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1491
augcccaagc agaaguccac ccgcuaccgg cuggcccucg accugggcag caccagccug       60

gggugggccg ugcuccgccu gaacgagcag cacgagccga acgcgaucau ccgggccggc      120

guccgcaucu ucuccaacgg gcggaacccc aaggacggca gcucccuggc cgugacgcgc      180

cgggccgccc gcgcgaugcg gcgccggcgc gaccggcucc ugaagcgcaa ggcccggaug      240

augaacaagc ugguggagua cggguucuuc cccgccgacg ucgccgagcg caagaagcuc      300

gagcggauca accccuacga gcugcgcgcg aagggccugg acgccccgcu cacccccggc      360

gaguucgccc gggcccuguu ccacaucaac cagcgccggg gguucaagag caaccgcaag      420

accgacaaga aggagaacga cuccggcgcc cugaaggcgg ccaucagcca ccuccgccag      480

cagcugcacg agcggaaggc ccgcaccgug ggggaguggc uguggcagcg gcuccaggcc      540

ggccugggga cgcgcgcccg guaccgcgag ucccgggugg cgcaggccaa cggcaagacc      600

cgcaucgaga aguccuacga ccuguacguc gaccggcaga ugaucgccga ggaguucgac      660

gcccucuggg ccgcgcaggc cgccuucaac ccccagcagu ucagcgagca ggcccgcggc      720

gagcugcggg acgugcugcu guuccagcgc gaccugcggc ccgugcgccc ggggcggugc      780

acccugcucc ccgacgagga gcgcgcgccc cuggcccugc ccagcgucca gcgguuccgc      840

auccuccagg aggccaacca ccuccggcug cuccacccgg accugcgcga ggagccccug      900

ucccucgccc agcgcgacgc cgugguggac gcgcuggagc acaacagcaa gcggaccuuc      960

gagcagcugc gcaagcuccu gaagcugaac ggcacgguca aguucaaccu cgaggacgag     1020

aagcggaagg agcugaaggg gaacgccacc uccgccaccc ugagcaagga cggccucuuc     1080

ggcgagaacu gguggaaccu ggacgaggcc cugcaggacg ccaucgugug gcagcucgug     1140

uccgagcaga gcgaggagaa gcugcagaac uggcugcagg cgaacaccgg ggucaacgcc     1200

acgcaggccg agaccaucgc caacgccggc cugcccgagg gguacggcuc ccucagcuac     1260

aaggcgcugg cccgcaucau ccccgcccug cgggggaccg agguggccaa cggcaucgac     1320

gugccgcgca ccuacgacca ggccguccag gcggccggcu ucgcccacca cagcgaccuc     1380

gacuccgggu ucgacuucga gcacagccac gacgaggugg agcggaucgg cgagcgcgug     1440

aucgccucca cgggggaggu caagcccgug uucgcguuca agcagcugcc cuacuacggc     1500

aaggcccugc ggcgccacgu ggccuucgcc aaggagcacc cccggaacga ggaggagcgc     1560

uacgggaaga ucgccaaccc gaccguccac aucggccuca accagcugcg gguggucgug     1620

aacgcgcuga uccgccggua cggccgcccc accgaggugg ucguggagcu cgcccgggac     1680

cugaagcaga gccgcgagca gcggcaggag gagcagaagc gccaggccga cuaccagaag     1740

cggaacgagc acauccgccg cgagaucgcc uccaugcacg ggugcgccga ggagcgggug     1800

agccacgcgg acguccagaa guggauccug ugggaggagc ucuccuucga cguggccgac     1860

cgccggugcc ccuacagcgg cgugcagauc uccgcccaga ugcugcugag caaccagguc     1920

gagaucgagc acauccuccc cuucucccag acccuggaca acagccugaa caaccgcacg     1980

guggccaugc ggcaggccaa ccgcaucaag gggaaccgga ccccguggca ggcgcgcgag     2040

gacuucgagc ggcagggcug guccuacgac agcauccucc agcgcgccga gcggaugccc     2100

aaggccaagc gcuaccgguu cucccgcgac ggguacgagc gguggcugaa ggaggacaag     2160

gacuuccugg cccgcgcgcu caacgacacc cgguaccuga gccgcguggc caagaccuac     2220

auggagcugg ucugcccccu cggcacgcgg gugauccccg gccagaugac cgcccugcug     2280

cgcgccaagu ucgggcucaa cgacgugcug ggccugaacg gggagaagaa ccgcgacgac     2340

caccggcacc acgccgucga cgcgugcgug aucggcguga ccgaccaggg ccuccugcag     2400

cgcuucgccc aggccuccgc cgacgcccgg gggaacggcc uggagaagcu cgucgagaac     2460

augccggugc ccugggcgac cuaccgcgac cacgugaagc gggccaucca gugcaucugg     2520

gucagccaca agcccgacca cggguacgag ggcccgauga uggaggagac ggccuacggg     2580

auccgcaagg acggcuccau ccggcagaag accaaggccg acggcagcac cgggcgcgag     2640

aucacccacc ugauccggau cacggagccc acccagaacc ugcgccacgg cguggacgcc     2700

caggggcagc cccucgcgua caagggcuac gucgggggca gcaacgacug caucgagauc     2760

acccagaacg agcagggcaa gugggagggg aacgugauca ccacguucca ggccuacgac     2820

aucgugcggc gccugggcuc caagcagggg ccccggcgcc ugcggcaccc gacccucgcc     2880

cagacccugg acguguacgg caagcccaag aagcugguca ugcgccucau gaucgacgac     2940

ugcgugcggc uggagcugga cgggaacaag aagacccucc gcguggucaa gaucaccggc     3000

aacggccagg uguucauggc ccccgugcac gaggccaacg ucgacaagcg gaacagcgac     3060

aagacggacc ccuucgcgua cguguccaag uacgccggga gcuuccagaa ggccaaggcc     3120

cgccggauca ccaucucccc gaucggcgag cugcacgacc ccggguucug a              3171


<210>  1492
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1492
augagcgaca aguccaagau ccugggcuuc gacaucggga ucaccagcau cggcugggcc       60

uacguggagg gggaggagcu caucgacugc ggcguccgca ucuucaccaa ggccgagaac      120

cccaagacga aggagucccu ggcgcugccg cggcgcgagg cccgcggggu gcggcggcgc      180

cucgcccggc gcaagggccg gcugaacgcc aucaagcagc ugaucugcaa ggaguucggc      240

cucaacuacc aggacuacau cgcccaggac ggggagcugc ccaaggcgua cgccggcaag      300

cugaucaacc ccuacgagcu ccgcagcaag gcccugcggg agaagcugga gaaggaggag      360

cucgcccgcg ugauccugca caucgcgaag caccgggggu acggcaacaa gcacgccaag      420

gagaccgacg acgccgaguc cgggaagauc aagaaggcca ucagcaacaa cgagaagauc      480

cugaacgaga agggcuacca guccgccggc gaguaccucu accaggaguu cuuccagaag      540

gagcgccugc aggaggagaa gaaggggaac aacaccacca aggaguucaa gaacguccgg      600

aacaaggcgg gcgacuacga gcacugcauc gcccagacgc agaugcagaa cgagcugcgc      660

cucaucuucg agaagcagcg ggaguucggg cugaacuuca gcgaggaguu caagaagaag      720

gugcuggaga ccgccuucuu ccagcgcccc cucaaggacu ucuccgacaa gaucggcgac      780

ugcaccuuca ucgagggcgc caagcgggcc ccgaaggaca gccuguccgc gaucgaguuc      840

guggcccugg ggcgcaucau caacacccuc aagaaccugc agaacacgag cggcgagguc      900

uacucccggg acaagaucaa cgagauccug cagaucgugc ucgacaaggg ggagaucagc      960

uacaagaagc ugcgcgagau ccuggacauc gaguccagcg ugcgcuucaa ggacuccaag     1020

cucgacuaca ccaaggagcu gaaggaggcc gagaagacca agcuguucga gagcaagaac     1080

cuccagaagu ucaagaaggc ccugggcucc uucgagggga ucagccggga gauccuggac     1140

agcaucgcca aggagaucau gaucaccaag gacaaggaga agcucgcgaa ggagcugaag     1200

aaguuccccc uggacgagac gcagauccag aaccucucca accugagcuu cucccagacc     1260

aucaaccuga gccucgaggc ccugucccag auccugcccu ucaugcgcgg cgagcgggag     1320

gacaagugcc uccgcuacga cgaggccguc gagaaggccg gccugaagga gguggccaag     1380

aacaagagca aggacgacuu ccugcccccg cucgagaacu acgagcccua ccuggcgaac     1440

cccguggucg cccgggcccu guccgaguac cgcaaggugc ugaacgcccu ccugcggaag     1500

uacgggaaga ugcacaagau ccacaucgag uucacccgcg aggugggcaa gagcaaggag     1560

gagcggaaca agaucgagaa ggagcagaag gagaacuucg cgaagaacca ggaggcccag     1620

aagcagugcg agauccuggg gcuccccauc aacggcgcca acauccugaa gaugaagcug     1680

uggcgcgagc agcaggaguc cugcgucuac agcgggaagg agaucgccau cgacgaccuc     1740

aagaacccgg ccauccucca gguggaccac aucuaccccu acucccggag cuucgacgac     1800

uccuacaaca acaaggugcu ggucuucagc aaggagaacc agaacaagcu gaacaagacc     1860

cccuucgagg cguucggcga gaacaaggac aagugggaga agauccuguc ccuggccagc     1920

aagcucccca agccgaagcg ccagcggauc uacaacaugc aguucaagga caaggagcag     1980

ggcuuccuga cgcgcaacau caacgacacc ggguacaucg cccggcuggu gucccagugg     2040

accaagaacu gccucagcuu cgagaagcug uccgaggacg aggugagcau cgccggcgag     2100

aaggggagca agguccacgu ggaggugauc uccggcagcc ugaccgccau gcuccgccac     2160

uacugggggc ugggcgacaa gaaccgggac aaccaccucc accacgcggu cgacgccauc     2220

aucaucgccu acuccaacga gaaggugauc aaggccuuca gcgacuacaa gaagcaccgc     2280

gagcagaaca aggccaaguu cuacgcgaag gagaucgccg aguccgagua caagaagcag     2340

aaggucuucu ucgagcccac gaaccacuuc cggcagaagg ugcuggagaa gaucgacagc     2400

aucuucgugu ccaagccgcc ccgcaagcgc gcccggggcg cccugcacga ggagaccuuc     2460

uacagcuucg agcgccugug caacgacaag gaguacgcga agaaggagaa gauggacuac     2520

gggggcaagg ccgggaucca gaaggccauc gcccucggca agauccggca ggucggcacc     2580

aagaucgugu ccaacgggac cauggugcgc gucgacaucu uccagcacaa gagcaacggc     2640

aaguucuacg gggugcccau cuacacgaug gacuucgccc ugggcgugcu gcccaacaag     2700

gcggucgugu ccgggaagga caagcagggc gugaucaagg acuggcucga gauggacagc     2760

aacuacgagu ucugcuucuc ccuguucaag gacgaccuga uccucgucca gaagaaggag     2820

auggagaagg ccgagcugug cuacuacacc gacuucaaca gcaacaccgc cggcaucaag     2880

uucgagaagc acaacaaccu guacgugaac cucaacgaga acgagcggaa gcuguucacc     2940

uccaaggagg uggucaaggg cgggaagaag aacgugugga aggacaagac gcccaaggag     3000

aucgagcagc uggagaagaa ccgcaagauc aucgggggcu ucgugaccau cgaggaccuc     3060

aagaccuucg agaaguacca ggucagcccc cugggggagg ugcagaaggc cgacuucgcc     3120

ccgcggcagg cgaucacccu gaaguccagc cccaagcacc gcaucaacaa guga           3174


<210>  1493
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1493
augagcaacc gcgugcccua ccggcuggcc cucgaccugg gcaccaacuc caucgggugg       60

ugccugcucg accugaacgg caacggggac ccgugcggcg uccgccggau gggggugcgc      120

aucuuccccg acggccggga ccccaagagc aaggaguccc uggccgcgga ccgccggcuc      180

gcccgcagca ugcggcgccg gcgcgaccgg uaccugcgcc ggcgcuccgc ccugaugaac      240

gcccucgugg ccgcgggccu gaugcccgcc gaccgggcca cccgcaaggc ccuggagagc      300

gaggacccgu gcgagcuccg ggcgcgcgcc cuguucgagc cccugacggc cgggcagguc      360

ggccgggccc ucuuccaccu gaaccagcgc cgcggguuca aguccaaccg gaaggccgcg      420

ggcgaggaga acgaggccgg gaagaucaag gccggcauca gcgcccugaa ggccgagauc      480

gaggcguccg gcgagaccac ccucggggcc uggcuggccg cccgccaccg gaagcgcgag      540

accgugcggg cccgccugga cggcgcgggg gccaaggcca gcuacgaguu cuaccccgag      600

cggggccucg ugcacgacga guuccaggcc auccgcacgg cccaggcgcu gcaccacccc      660

gccgucaccg ccgagcagug ggaccgggug gaguccguga ucuuccgcca gcggccgcug      720

cgccccgucc ggcccggcaa gugcacccuc uuccccgacc aggagcgcgc cccgugggcg      780

cugcccgugg cccaggaguu ccggauccug accgagaucg ggaaccucaa gcuguaccgc      840

cccggcgagg agccccggga gcugagcgcc gaccagcacg cccagcuguu cgccaagcuc      900

ugccgcuccg cggaccugac guucgagaag auccggaagg agcucaagcu ggacccgggg      960

cugaagguga accucgaggg cgagaagcgc gaccgccuga agggggacgc cgucaccagc     1020

gcccuguccg ccaagaagcg guucggcccc aaguggaacg cccucacccc cgacgaccgc     1080

cgggacaucg ugggccgccu gaucgaggcg gaggacgagg ccgagcuggc cggguggcuc     1140

gagagcgugc acggccugcc cuccgagaac gccgcccaca ccgcggccag cccggggcug     1200

cccgacggcu acugccggcu cuccgagaag gcccugguca agcuggugcc ccucaugcgc     1260

gagcagcggc ugcgcuacga cgaggccgug cuggcgcuga accuccacca cagcgacuuc     1320

cggcccgagg ggcugcugga cgagcucccg uacuacggcg agccccugga gcgcuucauc     1380

gccuucggca gcggggagcc cuccgacccg cccgagcagc accugggccg guuccccaac     1440

cccacggucc acaucgggcu caacgagcug cgcaagcugg ugaaccggau cguggagcgc     1500

uggggcaagc cggccgacau cgucguggag cucgcccggg accugaagcg cagccgggac     1560

gagcgcgaca ccauccagcg ggagcaggcc cagaaccagc gcaagaacga ggaccgguuc     1620

gcgaagauca aggagcuggg gaucgacccc cgccccgacu ccaugcuccg ccugcggcug     1680

ugggaggagc uccccaaggu gggcgucggc cacgugugcg ucuacaccgg ggagccgauc     1740

ggcauccgcc ggcugcugga gggggacgug gacaucgacc acauccuccc cuucagccgc     1800

acccuguccg acggcgccga caacaagauc gugugcgucc ggcgcgccaa ccgguucaag     1860

cgcgaccgga gccccuggga ggccuucggc gugacggccg uggacgggua cgacugggcg     1920

gccaucgccc agcgcgccga ggagcugccc aagaacaagc gguggcgcuu ccucccggac     1980

gccauggacc gguucgaggg cgagcgcggg uuccuggacc ggcagcuggu cgagacccgc     2040

caccucuccc ggguggcgcg ccaguaccug gccagcaucg uggaccugaa caagguccgg     2100

guggcccccg gcaagcucac cgccaugcug cgcgcgcgcu gggggcugaa caagauccuc     2160

uccgacggca accugaagaa ccggagcgac caccgccacc acgccaucga cgccgccgug     2220

cugggcauga ucgaccagcg gcucguccag cugguggccc aggcggccgc ccgcgccgag     2280

gccaacgagc uggagcgccu cuucgacggg cugcccgacc ccuucgacgg ccuggcggcc     2340

accuccuucg gggaccaggu gcgggagaag auccggggca ucacggucag ccaccgcgcc     2400

gagcacgggu ggcagggccg gaugcacaac gacaccgccu acggcccggu ggaggccgug     2460

aacggggucc acaccguggu gacccgcaag ccccucuccg gccugacggc gaaggacuuc     2520

cccaacaucc gggaccgcgu ccuggccaag gcccucgagg accacguggc cgccgaggcg     2580

gccgccgggc ggagcuucgg cgacgcccug ggguccuuca aguggggcgg ccagcccgug     2640

cgccgggucc gcaugcugga cgugcucggg aacccgaucc ccauccagga ccccagcggc     2700

accguguuca aggucuacaa gggggacucc aacgcguggu acgagauccu gcgggacccc     2760

cgcggccggu ggaccgggaa gaucgugagc accuucgagg ccuaccgccu gcaccgggag     2820

uggcucgccg agacgggcgg cgacuccggg cgcggcagcg ggguguuccu ggacugggcc     2880

gccaagcgcg ucgugggcga ggcgggcacc ccgcuguucc gccucuucaa gggggaccug     2940

cuggcccucg gcgaggcccc cgaccggcgg aucgugcgcg ucgugaaguu cagcgagggc     3000

cagguggccc uggcccugca ccucgaggcg gggaaccuga aggcccggga cgccgacaag     3060

ggggaccccu ucaaguacca guacugcucc cccgacagcc ugcgcaagca ggccgcccgg     3120

ccggcgggcg ucgaccuccu gggcuacgug cacgacaagg ggccccugga guga           3174


<210>  1494
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1494
augcgccggc ugggccucga caucgggacc aacagcaucg gcuggugccu gaucgaggac       60

gacaagaaga ucguggacau cgggguccgc aucuucuccg acggccggga ccccaagagc      120

ggggccuccc uggccgugga ccgccgggac gcgcgcgcca ugcggcgccg gcgcgaccgg      180

uaccucggcc gccggagcgc cuuccugcag gcccuggugg uccacggccu caugccggcc      240

gacgcggacg aggccaagcu gguggccgac cgcgaccccu accagcugcg ggccaaggcg      300

cuccacgugg cccuggagcc cuucgagauc gggcgcgccc uguuccaccu caaccagcgg      360

cgcggcuuca aguccaaccg gaaggccgac cgcaaggcca acgcggggga ggacggcaag      420

aucgccgagg gggccaaggc ccuggaccag gccaugagcg aggcgggcgu cgacacccug      480

ggccaguucc ucgcccugaa ggagacgaag cgggugcgcc ugggguccga cggccaggcc      540

uacgacuucu acccccagcg ccggcacauc gaguacgagu ucgaccgcau cuggaaggug      600

cagagcgagu accacccggc ccuccugacc gaggccgcgc gggccgcccu gcaccgcauc      660

cucuucuucc agcggccccu gaaggcccag gaggucgggu ucugcaccuu cgccggcgug      720

aacggcaucg cggccaacga gcgccggcug cccaaggccc acccccucuu ccagcagcgc      780

cggcuguacg aggaggugaa ccagcugcgc aucgucuccg ccggggcggc cgaccggccg      840

cucacccugg acgagcgcga ccggcugauc cuccgccugc aggaccggaa gaaggugagc      900

uucgacacgc uggccaagaa ggaccuccgc cuggccgacg gcgagcgguu caacaaggag      960

uccgagaacc gcaaggagcu gaucggggac gaggugcggg ccgagaugag cgacaagaag     1020

cgcuucggca cccgcugggu ccacuucggg cucgacgagc agcugcggau ccuggcgaag     1080

cuccaggacg aggagaaccc cgaggcccug cuggacuggc uccgcuccga guacggccug     1140

gacgaggccg ccgccgagag cguggcgaac gcccggcugc ccgagggcua cgggcgcuuc     1200

ggcgagaccg ccacccggcu ccugcuggac gcccucaagg ccgaggugau cacguacgac     1260

aaggcggccc ugcgcgccuu cgggcaccac uccgacuucc ggaccggcga gguccuggac     1320

cgccugcccu acuacgggga gcuccugacc cgggagaucg ccccgggcaa gcccgaguac     1380

ggcgaccccc uggagcgcca guacgggaag aucaccaacc ccacggugca caucggccuc     1440

cggcagcugg agaagcuggu gaacgcgguc aucgccgugc acgggcgccc ggaccagauc     1500

guggucgagc ucgcccggga gcugaagcug aacgagaagg agaaggacga gcacaagcgc     1560

cggaucaagc gcgacaccga cgccgccauc gcgcggggcc gcaagcucgc cagcgagggg     1620

uucaucgaca acggcgccaa ccggaugcug augcgcaucu gggaggagcu gaaccccgcc     1680

aacccccucg accggcgcug ccccuacugc gccgagccga ucggcauccg cgcgcuguuc     1740

uccggggagg ccgacaucga ccacaucauc cccuacagcc ggucccugga cgacaacgug     1800

ggcaacaagg ucguggccca ccgcagcugc aaccgggcca aggggaacaa gacccccuac     1860

gagaaguggg gcccccacau cgacccggag cgcugggagc ggaucagcga ccagguggcc     1920

cgccuccaca aguccaagca guggcgguuc ggccccgacg cgauggagcg caucgaccgg     1980

gacgggggcu uccuggcccg ccagcugacc gacacgcagu accucagccg ggucgccggg     2040

aaguaccugc gcucccugua ccccaccgcc cagagcggcc ggguggacgu gauccccggg     2100

cgcaugaccg cgaugcuccg gcgcaagugg ggccugaacu cccugcuccc ggaccacaac     2160

uacgucgaga acgagcacag caacgccccc aagaaccggc uggaccaccg ccaccacgcc     2220

aucgacgccg ccguggcggc cgugaccacg cugucccuca ugcagcagau cgccgacgcc     2280

gccggccggg cggaggacaa gaaccuggac cgccuguucg aggacauccc ccagccgugg     2340

cccggguucc gcgaggagcu cggcgaggcc cugcggagca ccaccgucuc ccacaaggcc     2400

gaccacgggc gcaagcggcg ccccagccgg gaccgcgacg ugaccgccgc ccggcugcac     2460

aacgacacgg cguacggccu caccggggac guggccgccg acggcaagac ccccaucguc     2520

gugcaccgcg ugccgcuggc cucccugaag cccgccgacc ucaucgaccc ccugcggauc     2580

cccgacccgg uccugcgcga cgcgcucuac gccgccaccc gggaccgcac gggcaaggag     2640

uucgagcagg cccugaccca guucgcgcgg caccaccccc uguucaaggg gauccgccgg     2700

gugcgcgugc gggagccccu caacgucauc cccauccgcg acgccgccgg ccgggccuac     2760

aaggccuaca agggggacgc gaacgcccgc uucgacgugu ggcggcugcc gaacggcaag     2820

ugggugagcg acaucgucuc cauguucgac gcccacugca uggccgacaa gagcgaccgc     2880

cgcccccacc ccgccgcgaa gaaggugcug ucccuccggc agaacgacau gguggccauc     2940

gagcgcagcg gggucaccca gaucgcccgg guggugaagu ucuccaccaa cggcagcauc     3000

gcccuggccg cggacaacga ggccggcgcc cugaaggccc gcgacgcggc ccccaacgac     3060

gucgacccgu ucaaguacau cuucuccagc gggggcgggc ucaagaaccu gaaggcccgc     3120

caggugcggg uggacccccu gggccggguc uucgaccccg ggccccgcga guga           3174


<210>  1495
<211>  3177
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1495
augaagaaca gcuacauccu gggccucgac guggggaucg ccuccgucgg cuacgggcug       60

auccacagcg acuccaagag cgugaucgac gccggcgugc gccuguuccc cgaggcgaac      120

gucgagaaca acgaggggcg gcgcgccaag cggggcuccc gccggcucaa gcgccggcgc      180

auccaccggc uggaccgcgu gaaguaccug cucgcccagu accaccugau caucgacgac      240

aagaucccca agagcaccaa ccccuacgcc auccggguga agggccuguc cagcgcccuc      300

uccaagagcg agcuggucau cgcgcugcuc caccuggcca agcgccgggg gauccacaac      360

gugaacgugg ucauggacga gaacgagucc accaacgagc ugagcacgaa ggagcagcuc      420

aaggagaacg ccaagcagcu ggagaaccgc uacgugugcg agcugcagcu cgaccggcug      480

aacgagcagu acaaggugcg cggcgagcgg aaccgcuuca agaccgagga cuucguccgg      540

gaggcccgcc agauccugac cacccagcag aaguaccacg acaucgacga gcacuucauc      600

gagcaguaca cgcaccuccu ggagacccgg cgcgaguacu acgaggggcc cggcaagggg      660

ucccaguacg gcugggaggg cgaccugaag cagugguacg agaugcucau ggggcgcugc      720

accuacuucc ccgaggagcu gcggagcgug aaguacgcgu acaccgccga ccuguucaac      780

gcccucaacg accugaacaa ccuggugauc gcccgcgacg agaacgagaa gcucgaguac      840

uucgagaagu accacaucau cgagaacguc uucaagcaga agaagucccc gacgcugaag      900

cagaucgcca aggagaucga cgugaaggag agcgacauca agggcuaccg gaucaccaag      960

aacgggaagc cccaguucac cuccuucaag cuguaccacg accucaacaa gaucuuccug     1020

gacaagaaga accuggagaa caucgagcuc cuggacgaga ucgcguucau ccugacccuc     1080

uaccaggacc cccugagcau caaggaggcc cuggaccagc uccaggaccu gcugacggag     1140

uccgagaagg aggccaucgc ccaccucaac ggcuacaccg gcacccaccg ccugagccug     1200

aagugccucc accuccugau cgacgagaug uggcagacca gccggaacca gauggaggug     1260

uucacggccc ugaaccucaa gccccagaag uaccagcugu ccaagcagaa gcagaucccg     1320

aagaagaugg ucgaggaguu cauccugagc cccgugguga agcgcuccuu cauccagagc     1380

aucgagaucg ucaacaccau caucaagaag uacgggcucc ccgaggagau caucaucgag     1440

cuggcgcggg agaagaacuc cgaggaccgc cggaaguucc ugaaccgcau ccagaaggag     1500

aacgagaaca cccggaagca ggucgaggag gugauccgcg aguacggcaa ccggaacgcc     1560

aaggggcucg uggagaagau caagcugcac cagaugcagg agggcaagug ccuguacagc     1620

cuccagaaca ucccccugga cgaccugcuc cgcaacccgu cccacuacga gaucgaccac     1680

aucauccccc ggagcguguc cuucgacaac agcuucaaca acaagguccu ggugaagcag     1740

gaggagaacu ccaagaaggg gaaccgcacc cccuaccagu accugaacag caacgagucc     1800

gugcucagcu acguccaguu caagcagcac auccugaacc uguccaagag caaggagcgg     1860

aucuccaaga agaagcgcga guaccuccug gaggagcggg acaucaacaa guucgaggug     1920

cagaaggagu ucaucaaccg caaccuggug gacacgcgcu acgccacccg ggagcucguc     1980

cagcugcuca agagcuacuu cuccgccaac gaccuggacg ugaaggugaa gaccaucaac     2040

ggcagcuuca ccaacuaccu gcgcaaggcc uggaaguucg acaagagccg gaacaagggc     2100

uucaagcacc acgcggagga cgcccugauc aucgccaacg ucuccuaccu cuucaagcag     2160

agcaaggagc ugaaggaggc caacgccauc cuggagaaca acaaccucuc ccugaacaag     2220

aacaaggaca gcaagaaccu gcucacggag caggacuacc gcaagauguu cgagaucccc     2280

aagcaggucc aggacaucaa ggacuacaag gacuucaagu acucccaccg gguggacaag     2340

aagccgaacc gccagcugau caacgacagc cuguacucca cccgggaggu gaacgagaac     2400

cacuacaucg ugcagaccgu gaaggacauc uacgcgaagg acaacaccaa gaucaaggac     2460

cucuuccuga agacgcccga gaagcugcuc auguaccagc acgaccccca gaccuucgag     2520

aagcugaugc uggucaucaa gcaguacggg gaggagaaga accccuucgc caaguacuac     2580

gaggagaacg gcgaguaccu caccaaguac gccaagaacg agaaggggcc gaccaucaag     2640

cgccugaagu acaucggcaa gaaggugggg acgcaccugg acaucagcac caacuucucc     2700

ggcgccaagc ggaagguggu ccagcucagc aucaagcccu uccgcuucga cguguuccac     2760

uccaaggcgg gcuaccacau gcugacccug accuacaugg acaucaagaa gaaggagaag     2820

cacuaccaga uccucaagaa cgaguaccag gcccugaagg agcgguacaa ggugcccagc     2880

gacgcccagu ucaucggguc cuucuacuac aacgaccuga ucagcaucaa cgacgagcug     2940

uuccgcguca ucggcgugaa caacuccacg cggaacgugg ucgagcugaa caugguggac     3000

aucaacuaca aggacuacug cgaccugaug gggaucaaga agaccccgcg caucuucaag     3060

accaucacca agaagaccgg caagaucgag aaguacagca cggacauccu cgggaaccag     3120

uucaagguga agccguccaa gagcccccag cugaucuuca agcggggcgu ccuguga        3177


<210>  1496
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1496
augcgccggc ugggccucga caucgggacc aacagcaucg gcuggugccu gacccagggg       60

cccggcgacg aggccgacgg gaucguggac aucggcgucc gcaucuucuc cgacggccgg      120

gacccgcaga gcgggacguc ccuggccgug gaccgccggg acgcgcgcgc cgcccggcgc      180

cggcgcgacc gguacaucgg ccgccggagc gccuuccucg acacccuggu ggcccacggg      240

cugaugcccg cggacgccga cgaggccaag cucgucgccg cgcuggaccc cuacacccug      300

cgcgcccggg cccucgaggc gccccugacc ccgcacgaga ucggccgcgc ccuguuccac      360

cucaaccagc ggcgcggguu caaguccaac cggaaggcgg agcgcaagac gaacgacgcc      420

gacgacggca agaucgccac cgccgccuac gcgcuggacc gggccaugcu ggacggcggg      480

uucgacaccc ucggccaguu ccugaacgcc caggagacca agcgcgugcg cauggacggg      540

gaggcccagg gcuacgacuu cuacccccag cggcgccacg uggaguacga guucgaccgg      600

aucuggacgg agcaggccaa gcaccacccc gcgcugcuca ccgagaccgc ccgcuacgcc      660

cugcaccgga uccuguucua ccagcgcccc cucaaggccc aggucguggg caagugcacc      720

uucgugcaca gcgaggaccg gcuggccaag gcgcacccgc uguuccagca gcgccggcuc      780

uacgaggagg ucaaccagcu ggaggugacg acccccgggg aggccaaccg caagcugacc      840

cucgacgagc gggaccgccu ggugcgggag cugucccaca agaaggccau cgccuucacc      900

agccucgcgg ccaagaucaa gcuggagccc ggccaguccu ucaacaaggc cagcgagacg      960

cgcacccagc uggccgggga cgagguccgg gccgugcucg gcgacaagaa gcgcuucggg     1020

cccgcguggg agcacuucga ccgggaccgc caguggacca ucguggagcg gcugcuggag     1080

gaggaggacc cggacgcccu ccacgccuuc cugacccagg acugcggccu ggacgacgag     1140

cacgccgccg cgacggccaa gaccucccuc cccgagggcu acgggcgccu gggcgccacc     1200

gccaccgccc gcauccuggc ggagcuccag gccgagguca ucacguacga cgaggccgug     1260

cggcgcugcg gguggcacca cagcgaccac cggaccggcg agauccugcc ccagcugccc     1320

uacuacgggg agcuccugac ccgcgacauc ccgcccggca cccaggaccc caacgacccg     1380

cccgagaagc gguggggccg caucacgaac cccaccgugc acaucgggcu gaaccagguc     1440

cggcagcucg ugaacgccau cguggcgguc cacggccgcc ccgaccagau cgugguggag     1500

cuggcccggg agcugaagcu gaacgagaag gacaaggagg agcacaaccg ccggauccgc     1560

cgggacaccg ccgccgccau caagcgcucc gagaagcuca cccaggaggg guuccccgac     1620

acgggcgcga accggaugcu gcugcgccuc ugggaggacc ugaacccggc caacccccug     1680

gaccggcgcu gccccuacug cggggccccc aucggcaugc gggcccucuu cgccggcgag     1740

gcggacgucg accacaucau cccguacagc cgcucccugg acgacagcgc cgggaacaag     1800

guggucgccc accgguccug caaccgcgcc aagggcaacc gcacccccua cgagaagugg     1860

agcgacgacc ccgaccggug ggacgccauc accgcccagg uggcccgccu gcaccggucc     1920

aagcaguggc gcuucgggcc ggacgccaug gcccgggugg agaaggacgg cggcuuccuc     1980

agccgccagc ugaccgacac gcaguaccug agccggcucg cggggcgcua ccuguccagc     2040

cuguacccca ccaaggagga gggcuccguc uacgugaucc ccgggcggau gaccgccaug     2100

cuccgccggc uguggggccu gaacgacauc cucagcgacc acaacaucgu ggagaacaag     2160

cacuccaacg cccccaagaa ccgccuggac caccggcacc acgccaucga cgccgcgguc     2220

guggccguga gcacccgcuc ccugaugcag cggaucgccc gcaccgccgg gcaggccgag     2280

gaccggaacc ucgaccgccu guucgagggc cuggacccgc ccuggcccgg cuuccgggac     2340

cagcuccgcg accgccugcu ggcggucacg gugagccaca aggccgacca cgggcggacc     2400

ggcacccccg ugccggggaa ggacgccacc gccggccgcc uccacaacga cacggccuac     2460

gggcugaccg gcgagaccuc cgcgaagggc cugcccaucg ucgugcaccg ggugccccuc     2520

accagccuga agcccgccga caucacggac ccggaccgca uccccgaccc cguccugcgg     2580

gacgccaucg agcgcgccac cgccgggaag augggcaagg acuucaccga cgcgcucgcc     2640

aaguucgggc gguccggcgc ccaggagacc gaccgcaagg gggagcggcu guuccgcggc     2700

acgcggcgcg ugcgggugcg cgagccccug ggcgucaucc cgaucgccga cgcgagcggg     2760

cggguguaca aggccuacaa gggcgacucc aacgcccgcu ucgacgugug gcggcucccc     2820

gacgggaagu gggucagcga caucgugucc auguucgacg cccaccgccc cgaccagggc     2880

gaccggcgcc cccacccggc cgcgaagaag gugcugagcc ugcggcagaa cgacaugauc     2940

gccaucgagc gcgacggggc gccccgcgag aucguccggg uggugaaguu cuccaccauc     3000

ggcagcgucc agcucgccgc caccaccgag gccggccccc ugaaggcccg cgacgccucc     3060

ccgcccgacg uggaccccuu ccgguacauc aacagcagcg ccgggggccu gaagaagauc     3120

aaggcccgcc aggugcggau cgacccccuc gggcgcaucu ucgacccggg cccccgguga     3180


<210>  1497
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1497
augaucgagc gcauccuggg cguggaccuc gggaucagcu cccugggcug ggccaucguc       60

gaguacgaca aggacgacga ggccgcgaac cggaucaucg acugcggggu gcgccuguuc      120

accgccgccg agacccccaa gaagaaggag agcccgaaca aggcccggcg cgaggcccgg      180

ggcauccgcc gggugcucaa ccgccggcgc guccggauga acaugaucaa gaagcuguuc      240

cugcgcgcgg ggcucaucca ggacguggac cuggacggcg agggcgggau guucuacucc      300

aaggccaacc gggccgacgu gugggagcug cgccacgacg gccucuaccg gcugcugaag      360

ggggacgagc ucgcccgcgu ccugauccac aucgcgaagc accggggcua caaguucauc      420

ggggacgacg aggccgacga ggagagcggc aaggugaaga aggccggcgu gguccugcgc      480

cagaacuucg aggccgccgg gugccggacg gugggcgagu ggcucuggcg cgagcgcggg      540

gcgaacggca agaagcggaa caagcacggc gacuacgaga ucuccaucca ccgcgaccug      600

cugguggagg aggucgaggc caucuucgug gcccagcagg agaugcggag caccaucgcc      660

accgacgccc ucaaggcggc cuaccgcgag aucgccuucu ucgugcggcc caugcagcgc      720

aucgagaaga uggucgggca cugcaccuac uuccccgagg agcggcgcgc ccccaagucc      780

gccccgacgg cggagaaguu caucgccauc agcaaguucu ucuccaccgu gaucaucgac      840

aacgagggcu gggagcagaa gaucaucgag cggaagaccc uggaggagcu gcucgacuuc      900

gccgugagcc gcgagaaggu cgaguuccgg caccuccgca aguuccugga ccucuccgac      960

aacgagaucu ucaaggggcu gcacuacaag ggcaagccca agaccgccaa gaagcgggag     1020

gccacgcugu ucgaccccaa cgagcccacc gagcucgagu ucgacaaggu ggaggcggag     1080

aagaaggccu ggaucagccu gcgcggggcc gccaagcugc gggaggcgcu cggcaacgag     1140

uucuacggcc gcuucguggc ccuggggaag cacgccgacg aggccaccaa gauccugacc     1200

uacuacaagg acgagggcca gaagcggcgc gagcucacga agcugccgcu ggaggccgag     1260

auggucgagc ggcucgugaa gaucggguuc uccgacuucc ugaagcugag ccucaaggcg     1320

auccgcgaca uccugcccgc cauggagucc ggcgcccggu acgacgaggc cgugcugaug     1380

cucggggucc cccacaagga gaaguccgcc auccugcccc cgcugaacaa gaccgacauc     1440

gacauccuca accccaccgu gauccgcgcg uucgcccagu uccgcaaggu cgccaacgcc     1500

cuggugcgga aguacggcgc cuucgaccgc gugcacuucg agcuggcgcg ggagaucaac     1560

accaagggcg agaucgagga caucaaggag agccagcgca agaacgagaa ggagcggaag     1620

gaggccgccg acuggaucgc cgagacgagc uuccaggucc cccucacccg caagaacauc     1680

cugaagaagc ggcuguacau ccagcaggac gggcgcugcg cguacaccgg cgacgugauc     1740

gagcuggagc ggcuguucga cgagggguac ugcgagaucg accacauccu gccccgcucc     1800

cggagcgccg acgacagcuu cgccaacaag gugcugugcc ucgcccgcgc caaccagcag     1860

aagaccgacc ggacgccgua cgagugguuc ggccacgacg cggcccgcug gaacgccuuc     1920

gagacccgga ccuccgcccc cagcaaccgc guccggaccg ggaagggcaa gaucgaccgc     1980

cugcugaaga agaacuucga cgagaacucc gagauggccu ucaaggaccg gaaccucaac     2040

gacacgcgcu acauggcgcg ggccaucaag accuacugcg agcaguacug gguguucaag     2100

aacagccaca ccaaggcccc cgugcagguc cgcuccggca agcugaccag cgugcugcgc     2160

uaccaguggg ggcucgaguc caaggaccgg gagagccaca cgcaccacgc cguggacgcc     2220

aucaucaucg cguucuccac ccagggcaug guccagaagc ugagcgagua cuaccgcuuc     2280

aaggagaccc accgggagaa ggagcgcccc aagcuggccg ugccgcucgc caacuuccgg     2340

gacgccgugg aggaggcgac ccgcaucgag aacacggaga ccgucaagga ggggguggag     2400

gugaagcggc ugcugaucuc ccgcccgccc cgggcccgcg ucaccggcca ggcccacgag     2460

cagaccgcca agcccuaccc ccggaucaag caggucaaga acaagaagaa guggcgccuc     2520

gcccccaucg acgaggagaa guucgagagc uucaaggcgg accggguggc cuccgccaac     2580

cagaagaacu ucuacgagac gagcaccauc ccccgcgugg acguguacca caagaagggc     2640

aaguuccacc uggugcccau cuaccugcac gagauggucc ucaacgagcu gccgaaccug     2700

ucccucggga ccaaccccga ggccauggac gagaacuucu ucaaguucag caucuucaag     2760

gacgaccuga ucuccaucca gacccagggc acccccaaga agccggccaa gaucaucaug     2820

ggguacuuca agaacaugca cggcgcgaac auggugcuga gcuccaucaa caacagcccc     2880

ugcgaggggu ucacgugcac ccccgugagc auggacaaga agcacaagga caagugcaag     2940

cucugccccg aggagaaccg gaucgccggc cgcugccugc agggcuuccu ggacuacugg     3000

ucccaggagg ggcuccggcc gccccgcaag gaguucgagu gcgaccaggg cgucaaguuc     3060

gcccuggacg ugaagaagua ccagaucgac ccccuggggu acuacuacga ggugaagcag     3120

gagaagcggc ucggcaccau cccccagaug cgcagcgcca agaagcuggu caagaaguga     3180


<210>  1498
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1498
augagcaagc gccugcggua ccgccucgcc cuggaccugg gcaccaacuc caucggguuc       60

ugccuccugg accugaacgg cgacgggcgg cccugcggcg ugcgccggau ggggguccgc      120

aucuucccgg acuucagcaa gcuccagcag ucccuggccg cggaccggcg ccuggcccgg      180

agcaugcgcc ggcgccggga ccgcuaccuc cggcgccgga aggcccugau gaacgcccug      240

guggccgcgg cccucaugcc cgccgacccc aaggcccgca aggcgcugga ggcccuggac      300

cccuacgagc uccgggccgc cgccguggcg ggcccgcugc ccgcccacca gcugggccgc      360

gcccucuucc accugaacca gcggcgcggg uucaagucca accgcaaggc cgccggcgac      420

gagaacgagg cggggaaggu caagagcggc aucuccgccc ugaaggccga gcucgccgcc      480

agcggggcgg gcacccuggg ccaguggcug gccgcccggc acgccaaccg ccagcccgug      540

cgggcccgcc ucacggggac cggcgcgaag gccaccuacu ccuucuaccc cgagcgggcc      600

cuggugcacg aggaguucca ggccauccgc gcggcccagg ccccgcacca ccccgggguc      660

accgccgagc agugggaccg gaucgagagc gugaucuucc accagcgccc ccugaagccc      720

gugaagccgg gcaagugcac gcucuacccc gaggaggagc gggcccccug ggcgcugccc      780

aucgcccagc gcuuccggau ccugcacgag cuccacaacc ugcgcaucgu ccggccgggc      840

cagggggagg agggccuguc ccccgccgac uucgaccgcu ucuucgaccg gcucugccgc      900

aagggggacc ugaccuucaa gacccuggcc aaggagcuca agcugccgcc cgacgcccgg      960

aucaaccugg aggacgacaa gcgcgaccgg cucaagggcg acgcgaccgc cgacgcccug     1020

agcgccgaga aggggaagcg ccgccggauc ggcgccgcgu ggcacuccuu ccccgccgac     1080

cgccagcggg agaucguggg ccgccugauc gacgccgagg acgaggggga gcucgccgcg     1140

uggcuggugg aggagcuggg ccuccccugg gaggucgcca uccugacggc cgagcggccc     1200

gggcugcccg acggcuacug ccgccucagc gagaaggccc uggagcaggu cgugccgcug     1260

augcacgagg agcaccuccc cuacgacgag gccgugcggg cgcucgggcu gcaccacucc     1320

gaccaccgca ccggcgaguu ccuggacgag cuccccuacu acggccugcc ccuggagcgg     1380

uucaucgccu ucgggaccgg cgaggccgac gacccgcccg agcuccgcau cgggcgguuc     1440

cccaacccga ccgugcacgu ggcccugaac gagcugcgcc gggucgugaa ccgcaucauc     1500

cggcgcuggg gcaagcccga ggagaucgug gucgagcucg cccgggaccu gaagcagagc     1560

cgcgcggacc gggacaagac gcagcgcgag caggccgaca accagaagaa gaacuggacc     1620

cggacccagc agcugcgcga gcucgggaug gagcccaccg gcgaggcccu gcugcgccuc     1680

cggcuguggg aggagcugcc ccgcaucggc uccgcccacg ugugcgugua cacggggcag     1740

ccgaucagca ugacccagcu ccugcagggc gacgucgacg uggaccacau ccugcccuuc     1800

ucccggaccc ucgccaacgg ggcggacaac aagaucgucu gccaccgccg ggccaaccgc     1860

aucaagcgga accgcacccc cugggaggcc uucggcgugg ugagcgccga cggcuacgac     1920

ugggcggcca ucgccgagcg ggccgagagc cugcccaaga acaagcgcug gcgguuccug     1980

ccgaacgcca uggagcgcuu cgagacggag cggggguuca ucgaccgcca gcucgucgag     2040

acccggcacc ugucccgcgu ggcgcgggag uaccugggcu gcaucgugcc ccucgagaag     2100

guccgcgccg cccccgggcg gcugaccgcc augcugcgcg gccgcugggg gcucaacagc     2160

auccuguccg acgccaaccu gaagaaccgg agcgaccacc gccaccacgc gguggacgcc     2220

gccgugcucg gcgucaucga ccagcggcug gugcagcgcg uggccgacgc cgcgaagcgg     2280

gccgaggaga aggagcugga gaagcuguuc gacggccugc ccgacccguu cgaggggcug     2340

cccggccccu ccuuccgcga ccagguccag gagcgggccc aggggaucau cgugagccac     2400

aagcccgagc acggcuggca ggggcgcaug cacaacgaca ccgccuacgg cccgguggag     2460

cggcuccccg acggcacgca ccgcgucgug acccggaagc cccugggguc ccuggccgag     2520

aaggacuucg gcaacauccg cgaccgggcg cucgccgccg cccuggagcg ccacguggcg     2580

gagcaggccg cccaggggcg gagccugggc gaggcccucu ccugcuucac cuggaccggg     2640

ggcgaggucc gccgggugcg caugcuggac aagcugggca gccccguggc cguccaggac     2700

gacgacgggc acacguucaa gcucuacaag ggcgacucca acgcguggua cgagauccug     2760

cgggacgggc gcggccggug gaccgggcgc aucgugagca ccuacgaggc cuaccgccug     2820

cacgccgagu ggcucgccga gcggggcgac ggcgggcgcu ccgacgccgc guucccggac     2880

ugggccgccc ggcgccugug cgccggcauc gggaagcccc uguuccgccu cuucaagggc     2940

gacaugcugg ccuucggcac cgcgcccaac cgggagauca ugaagguggu caaguucagc     3000

gaggggcgga ucguguucgc cggccaccgc gaggccggga accugaagga gcgggacgcc     3060

gacaaggacg accccuucaa guaccgcacg ugcuccccgg aggcgcuccg gaaggcccag     3120

gcccgccccg ugggcgucga ccccgccggg uucgugcacg accggggccc gcuggaguga     3180


<210>  1499
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1499
augggcaucc gcuucgccuu cgaccugggg accaacagca ucggcugggc cguguggcgg       60

accgggcccg gcgucuucgg ggaggacacg gcggccuccc ucgacggcag cggcgugcug      120

aucuucaagg acgggcgcaa cccgaaggac ggccaguccc uggccaccau gcggcgcgug      180

ccccggcaga gccgcaagcg gcgcgaccgg uucguccucc gccggcgcga ccugcuggcc      240

gcccuccgga aggcggggcu guuccccgug gacguggagg agggccgccg gcuggccgcc      300

accgaccccu accaccuccg cgccaaggcg cuggacgagu cccugacccc gcacgagaug      360

gggcggguca ucuuccaccu caaccagcgc cggggcuucc gcagcaaccg gaaggccgac      420

cgccaggacc gcgagaaggg caagaucgcc gaggggucca agcggcuggc cgagacgcug      480

gccgcgacca acugccgcac ccucggcgag uuccugugga gccggcaccg cgggacgccc      540

cggacccgcu cccccacccg gauccgcaug gagggcgagg gcgccaaggc ccuguacgcc      600

uucuacccca cccgggagau ggugcgcgcc gaguucgagc ggcucuggac cgcgcagagc      660

cgcuucgccc cggaccugcu gacgcccgag cggcacgagg agaucgccgg gauccucuuc      720

cgccagcggg accuggcccc gcccaagauc ggcugcugca ccuucgagcc cuccgagcgc      780

cggcugcccc gcgcccuccc cagcguggag gcgcggggga ucuacgagcg ccuggcccac      840

cuccgcauca ccaccggccc cgucuccgac cgggggcuca cgcgcccgga gcgggacgug      900

cuggccagcg cccugcucgc cggcaagucc cugaccuuca aggcggugcg caagacccug      960

aagauccucc cccacgcccu ggucaacuuc gaggaggccg gcgagaaggg gcuggacggc     1020

gcccucaccg cgaagcugcu gagcaagccc gaccacuacg gggccgccug gcacggccuc     1080

uccuucgccg agaaggacac guucgugggg aagcugcugg acgaggccga cgaggagcgg     1140

cucauccgcc ggcuggugac cgagaaccgc cugagcgagg acgcggcccg gcgcugcgcc     1200

uccauccccc ucgccgacgg cuacggccgg cuggggcgca ccgccaacac cgagauccug     1260

gcggcccucg ucgaggagac ggacgagacc ggcaccgugg ugaccuacgc cgaggccguc     1320

cggcgcgccg gggagcggac gggccgcaac uggcaccaca gcgacgagcg ggacggggug     1380

auccuggacc gccugccgua cuacggcgag auccugcagc ggcacguggu ccccggcagc     1440

ggggagcccg aggagaagaa cgaggcggcc cgcuggggcc ggcucgccaa ccccaccgug     1500

cacaucgggc ugaaccagcu gcgcaaggug gucaaccgcc ucaucgccgc gcacggccgg     1560

ccggaccaga ucguggucga gcuggcccgc gagcugaagc ucaaccggga gcagaaggag     1620

cgccuggacc gggagaaccg caagaaccgg gaggagaacg agcgccggac cgccauccug     1680

gccgagcacg gccagcgcga caccgccgag aacaagaucc ggcuccgccu guucgaggag     1740

caggcgcggg ccaacgccgg gaucgcccug ugccccuaca cgggccgcgc caucgggauc     1800

gcggagcugu ucaccuccga gguggagauc gaccacaucc ugcccgugag ccugacccuc     1860

gacgacuccc uggccaaccg gguccugugc cgccgggagg ccaaccgcga gaagcggcgc     1920

cagacccccu uccaggccuu cggcgccacg ccggcgugga acgacaucgu ggcccgcgcc     1980

gccaagcucc cgcccaacaa gcgguggcgc uucgaccccg cggcccugga gcgguucgag     2040

cgcgagggcg gguuccuggg ccggcagcuc aacgagacca aguaccugag ccgccuggcc     2100

aagaucuacc ucgggaagau cugcgacccc gaccgggugu acgucacccc cggcacccug     2160

accgggcugc uccgcgcccg guggggccug aacuccaucc ugagcgacuc caacuucaag     2220

aaccgcagcg accaccggca ccacgccgug gacgcggugg ucaucggggu gcucacgcgc     2280

ggcaugaucc agcggaucgc ccacgacgcc gcccgcgccg aggaccagga ccuggaccgg     2340

guguuccgcg acguccccgu gccguucgag gacuuccggg accacgugcg cgagcggguc     2400

uccaccauca ccguggcggu gaagcccgag cacggcaagg gcggggcccu gcacgaggac     2460

accagcuacg ggcucguccc cgacacggac cccaacgccg cccugggcaa ccugguggug     2520

cgcaagccga uccggucccu caccgccggg gaggucgacc gcgugcgcga ccgggcgcug     2580

cgcgcccggc ugggcgcccu cgccgccccc uuccgcgacg agagcggccg ggugcgcgac     2640

gcgaaggggc uggcccaggc ccuggaggcc uucggcgcgg agaacgggau ccggcgcguc     2700

cggauccuca agcccgacgc cuccguggug accaucgccg accgccggac cggcgucccc     2760

uaccgcgccg uggccccggg ggagaaccac cacguggaca ucguccagau gcgggacggc     2820

agcuggcgcg gcuucgcggc cuccguguuc gaggugaacc ggcccgggug gcgccccgag     2880

ugggagguca agaagcuggg cgggaagcug gugaugcggc uccacaaggg cgacauggug     2940

gagcugagcg acaaggacgg ccagcgccgg gucaaggugg ugcagcagau cgagaucucc     3000

gccaaccgcg uccgccugag cccccacaac gacgggggca agcuccagga ccggcacgcc     3060

gacgccgacg acccguuccg cugggaccug gcgacgaucc cccugcucaa ggaccggggg     3120

ugcguggccg ugcgcgucga ccccaucggc guggugaccc ugcggcgcuc caacgucuga     3180


<210>  1500
<211>  3183
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1500
auggccguca agagccugaa cuacauccuc ggccuggacc uggggaucgc cuccugcggc       60

ugggcggugg uggagaugga cgagcaggag aacccccucc gccugaucga cguggggguc      120

cggaccuucg aggaggccga gaacccgaag gacggcagcu cccuggccga gacccgccgg      180

cucgcccgcg cccagcggcg ccugaucagc cggcgcgcga accggaugaa caagcugcgc      240

cggcuccuga agcaggagaa cgugcugcag gccaacgacu acgacgagaa ggggcucauc      300

aucggccugc ccaaccaggc cugggagcug cgcgcccagg gccucgaccg gaagcuggag      360

cccaaggagu gggcggccgu gcugauccac cucgucaagc accgcgggua ccugucccag      420

cggaagaacg agagccagac ggccgacaag gagcugggcg cccuccuguc cgggaugaac      480

cacaaccacc agcugcucca gagccagucc aaccaguacc gcacccccgc cgagcuggcg      540

aucaagcagu ucgccaccca ggacggccac auccggaacc agcgcggggc cuacacccac      600

acguucaacc ggcuggaccu ccagaacgag cugaaccagc uguucgacgc ccagacccgc      660

cucggcaacc cgcacgugag cggcucccug aagaccgcca ucaacgaccu gcucaugaac      720

cagaaggcgg cccugagcgg ggacgccauc cuggagaugc ucggccggug caccuucgag      780

cccuccgagu acaaggccgc caagaacacg uacagcgcgg agcgcuucgu guggcugggg      840

aagcugacca accuccgcau ccaggacaac ggccuggagu ccgcccugag ccgggagcag      900

cgcgagcucc ugcugaacga gcccuacaac aagaccaagc ucaccuacca gcagguccgg      960

aagcugcugc gccuccccga gacggccuuc uucaagggcc ugcgguacgg gaaggacaac     1020

gccgaguccg ccacccugau ggagaugaag uccuaccacg cgauccgcaa gagccuggag     1080

aacaagagcc ucaagagcga gugggagaag cugaagaccc agccgaagcu gcucgaccug     1140

aucggcaccg ccuucuccau cuacaagacg gacgacgaga ucgccgggug ccugaagaac     1200

cagcuccccg agaacgugau caucgcccug caggagcagc ugaacuucag caacuucaug     1260

gagcucuccc ugaaggcgcu ggagaagcuc cugccccuga uggagcaggg ccuccgguac     1320

gacgaggccu gcacccagau cuacgggaac cgccacgaga agaucgccga caagaccgag     1380

aaguuccugc ccaacauccc ggccgacaag cugcggaacc ccgugguccu ccgcacccug     1440

acgcaggccc ggaaggugau caacgcgauc auccgccggu acggcagccc cgcccgcgug     1500

cacaucgaga ccggccggga ggucgggaag uccuucagcg accgcgacaa gcugaagaag     1560

cagcaggagg agaaccagga gcagcggcgc cgggccaucg acgaguggaa gcaguucuuc     1620

cccaacgccg cccacgagcc gaaggcggug gacauccuca agcugcgccu guaccagcuc     1680

cagcagggca agugccugua cuccgggcug cccaucgacg ugcgccggcu ccccgagaag     1740

ggcuacgucg agaucgacca cgcccugccc uucagccgca ccugggacga cuccuucaac     1800

aacaagaucc uggugcucgc cagcgagaac cagaacaagg ggaaccagac cccguacgag     1860

uaccugcggg gcgccgacaa cuccgagagc uggcgccugu ucguggagaa cguccagaac     1920

ugccaguucu cccccaacaa gaagcagaag aucauggcca agcagcucga cgagaagggc     1980

uuccuggagc ggaaccugaa cgacacgcgc uacgugagcc gguuccucug ccaguucauc     2040

uccgaguacc ugcaccugac cgggaagggc aagaagcgcg uguucgcgag caacgggcag     2100

aucaccgccc uccugcgggg ccgcuggggg cuguccaaga accgggagga gaacgaccgc     2160

caccacgccg ucgacgccgu ggucguggcg ugcagcaccg uggccaugca gcagaagauc     2220

acgcaguucg uccgguacca gaagggcaac guguucaccg gcgagcgcau cgacaaggag     2280

accggggagg ugaucuccau ccacuucccc acccccuggg agcacuuccg ggaggagguc     2340

augauccgcg uguucgacga caacccgcuc cggacgcugc ccgagaagcu gcccaaccgc     2400

cccgaggccc uccacgagua cgugaugccg cuguucguca gccggaugcc cacccgcaag     2460

augaccggcc aggggcacaa ggagaccauc aagagcgcca agcggcugga cgagaagauc     2520

uccgugcucc gcgugccccu gacgcagcug aagcucaacg accuggagaa cauggucaac     2580

cgcgagcggg agccggcccu guacgaggcg cucaaggccc gccuggagca guucaagaac     2640

gaccccgcca aggccuucgc cgagcccuuc uucaagcagg gcggccagca ggucaaggcg     2700

gugcgggugg agaucgugca gaaguccggg augcugcucc gcaagaucaa cggcguggcc     2760

gacaacggga gcaugguccg gguggacgug uucgagaagu ccggcaaguu cuaccugguc     2820

cccaucuacg ccuggcaggu cgccgagggg auccugccga accacgccgu ggugcacagc     2880

aaggacgaga gcgacuggau ccucauggac gaguccuacc acuucaaguu cgcgcuguac     2940

aagaacgacc ugcucgaggu gcagaccaag aagggcugca ucaugggcua cuucgccagc     3000

cuggaccgcg ccaccggggc caucaccauc cgggagcacg accgcgacaa gaagaagugc     3060

aaggacggcg agcaccgguc caucggggug aagauggcga gcuccuucaa gaagcugcag     3120

gucgacgagc ucggcaagaa cauccacgag acccgcagcc ggaagcgccc caacaucggg     3180

uga                                                                   3183


<210>  1501
<211>  3186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1501
augcacaccc ugcccuacac ccucggccug gacaucggga ucgccagcgu gggcgccgcg       60

cugcucuccg acgagagcau ccuggggaug cacguccgca cguucuccgc cgccgagacc      120

gacaaggagg gcgacccgcu gaacaagauc cggcgcgagg cccggagcgc ccgccggcgc      180

auccggcgcc gggcgcaccg ccuccugcgg cugcgccggc uccugaagcg caccgggcug      240

aucuccgaca ccagccucga ggugcugaag acguccaaga cccccuggca gcugcggagc      300

gagggccucg accgccugcu gucccccagc gagcucgccg ccgcccugua ccacaucgug      360

aagcaccggg gcuuccaguc caaccgcaag gcggagcuga agaccgacgc caaggccggg      420

cagaugcuca gcggcgucuc cgccaaccag cagcugaugc gggacaagag cuaccgcacc      480

aucgccgaga uggugugcaa ggacgacgcg uucaaggacc acaagcggaa caagggcggg      540

uccuaccgca acacguucgc ccgcagcgac cuggccgacg agcucaagac ccugcugaag      600

aagcagcggg aguacgacaa cccccucgcc accccggagc uggaggaggc cgugcacacc      660

cugcucaugg cgcgccggcc cgucuucaag gacgacgaca ucgagaagcu ggugggguuc      720

ugcacguucg agcacggcga gaagcgcgcc cccaaggccg ccuucuccag cgagcgguuc      780

aucuggcugg gcaagcucaa caaccugcgc cugaucuacc ccggggagca gcggucccuc      840

accccggagg agcgcagcca gaucaucaac cugcccuuca cccaggccag cgugaaguuc      900

ucccagaucc ggaagaagcu ggaccucccc gaguacgucc gcuucaaccu ggugcgguac      960

aagcccggcg aggagaccag caaguccgag acggcgaccu ucuucgaggc caaggccuuc     1020

cacgagcugc gcaagaccua ccgggagagc gggaucuccg agcucuggga gcgcgaccag     1080

cacgacccgc agcggcugga ccagaucgcc accgccaucg cgguguaccg cgacgacgag     1140

cggauccgca acgagcugag cccccacacg aucgagaacg aggucgugga ggccgugcuc     1200

aacugcgacu ucacccaguu caucaaccug ucccugaagg cccucaacaa gauccugccc     1260

uacauggagc agggccagcg guacgacgag gccugcacca gcgcgggcua ccagcaccac     1320

agcccccagc agaacgagga gaaguccggg cgcaucccgc cgcccgacaa gaacgucauc     1380

cgcaaccccg ugguguaccg gucccugaac caggcccgca agcugaucaa cgccaucacc     1440

aagcgguacg gcgcccccgc cgcgauccac cucgagaugg cccgcgaccu gagcaagccc     1500

uucuccgagc gcaagagcau cgagcgggag caggagaagu accggaagca gaagguccag     1560

cugcgcaacg aguacaucga gcacuucggg acggagccca acggccucaa ccugcugaag     1620

uuccggcucu acaaggagca ggacgcccag ugcgccuaca cccagaaggc ccuggaccug     1680

ucccgccuca acgaggcggg guacguggag guggaccaca uccugcccua cagccggucc     1740

uacgacgaca gccugcucaa caagacccug guccugaccg aggccaaccg cgacaagggc     1800

aaccggacgc cguucgaguu caugggcggg gacgaccagu cccagcacug gcagaaguuc     1860

gugcgcuggg ugcagaugaa ccccaagauc cggcaggcca agcgcaaccg guaccuccgc     1920

aaggacuuca gcgagaaggc cgcccgggag uuccgcgacc ggcaccugaa cgacacccgc     1980

uacaucggcc gcgagcugaa ggcgaugauc gagagccacg uccaguggca ccccgacucc     2040

aagagcaagg acugcugcgu gguccucucc gggcagcuga ccgcccugcu ccggggccgc     2100

uggggguuca acaaggaccg ggccgccggc gaccugcacc acgcggugga cgccgccgug     2160

gucgccgccg cgacccgcgg caugauccag cggauggccc gccacagcaa guuccacgag     2220

cugaagaacg ugcggacguc cuacgccgac cccgacaccg gggagguggu caaccucgcc     2280

gccgcgcgcg accuggagaa gcacuucccg cugcccuggg acaacuuccg gaacgacacc     2340

cucgccuacc ugaaccccag cgccaucucc gccgacgaga gcgcccagga gggccaccgc     2400

ggggugcggg ugucccgcgc gccgacccgg cgcaagcugg gcgccgccca ccaggagacg     2460

auccggagca aggggaagga caacauccuc cuggagcagg gccuguccgc cgucaagacg     2520

ccccucagca agcugacccu gaagaacauc gagaacaugg ugggcuacga ggacccccgc     2580

aacgcggccc ucgugaaggu ccugcgggag cgccuggagg ccuucggggg cgacggggcc     2640

aaggccuucg cgcccaacca gccgcccgug uacaagcccu ccaagcccgg caagcaggcc     2700

ccggagaucc ggagcgugaa gauggccucc aagagcaagg gcgcccucca cguccgcgag     2760

gggcuggccg acuccggccg caugauccgg aucgacaucu acgagaccaa gaacggguac     2820

gugggcgugc ccaucuacgu cagcgacaag gcgcgcagca agcugcccaa ggccuacccc     2880

aagaaggggg aggaccgggc caacuggccg gagguggaca ccaacgacaa cuuccucuuc     2940

ucccuguaca gcaccgacug gaucgaggcc aagaccaaga aggagaccau cgccggcuac     3000

gugaaguccu acgacaucuu cgacggccgc cugcucgucu acgcgcacga caacaagaag     3060

guggugcgga agcugacccu gagcgcccuc gccgaccugc agaagcugca cgucuccauc     3120

cucggggacc uguacaaggg cgggacgccc aagugcagcc cccugcgcaa gggccaggag     3180

cucuga                                                                3186


<210>  1502
<211>  3186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1502
augcugcgcc ucggccugga caucgggagc accuccaucg gcugguuccu guaccggauc       60

accaacggga ugaucgccga gaucaucgac ugcggcgugc gccucuucag cgacgggcgg      120

gacgccaaga cgggcgucuc cagcgcggug gagcgccgga acgcccgcgc ccugcggcgc      180

cggcgcgacc gguaccugcg ccggcgcgcc gcccucaugc ggcgccuggc ggccgccggc      240

cugaugcccg aggacccguc ccaggccaag ccccucgagc ugcuggaccc cuaccggcuc      300

cgcgcggacg cccuggaccg gcgccuggag cucaccgagc uggggcgggc ccuguuccac      360

cucaaccagc gccgcggcuu caagagcaac cggcagaccg accgcgggaa caacgagggc      420

gggaagauca ucgacggcuc cgcccggcug gaccaggcca ugauggcggu gggcgcccgc      480

accuacgggg aguuccugca ccuccggcgc agccaggccg aggaccugcg gcgcgucccc      540

uccgugcgga cgcgccugac ccuccggagc guggacggcg ggaaggccga gaccggcuac      600

gacuucuacc cggaccgccg gcaccuggag gaggaguuca acaaggucug ggccgcgcag      660

gccgcccacc accccgugcu gaccgacucc cuccgccacg agguguucga gacgaucuuc      720

uuccagcggc cccugaaggc ccccgccguc ggccgcugcc uguucuuccc ggagccccgg      780

cucccccgcg cgcacccccu gacccagcgg cgcgugcugu acgagaccgu gaacgcccuc      840

cgcauccgga ccgagagcgg ggccgccguc ccgcugggca aggagcagcg cgacaaggug      900

aucaugcugc ucgacgggaa ggcccccgug aaggcgaucg ccuccgccaa gcugagccug      960

aaggccaucg cgaagcaccu caagcugcgg gacggccaga cguucacccu ggagaagggg     1020

cgcgacuaca ucgccugcga ccccguccgg gccucccuca gccaccccga ccgcuucggc     1080

ccgcgguggu ccgugcugga ggccgaggug caggccgaga ucgucggccg cauccgggac     1140

gacgacgaca ccauggcgcu gguggagugg cucaagcagg uccacgggcu ggacgacgcc     1200

cacgcccgcg ccaccgccga cgcgccccug cccgagggcu acgggcggcu cggcgagacg     1260

gccacccgca agcugcugga cugccugaag gccgaggugc ucaccuacag cgaggccgug     1320

gggaaggccc uggaccagca ccacuccgac cggcgcaccg gcgagauccu ggagagccuc     1380

cccuacuacg gcgaggugcu gcaggcgcac gucaucccgg ggacgggcga gccccaccac     1440

gacgacauca cccgguucgg gcgcaucacc aaccccaccg ugcacaucgg ccugaaccag     1500

cuccggcgcc uggucaaccg gaucaucgcc cgcuacggga agcccgccga gaucguggug     1560

gagcuggccc gggaccucaa gcagagcacg ggccagaaga aggcggccau cgcccugaac     1620

cugaagaacc gcaaggccgc cgaggcgcgc uccgcccucc ugaccgagug gggcgucccg     1680

gacaccgggg ccaaccggau guacgugcgc cuguggcagg agcuccacga ggaccccaug     1740

augcggcgcu gccccuacac cggcacgauc aucagccccu cccugcuguu cagcggggcc     1800

aucgacaucg accacauccu cccguacucc cggacccugg acgacagcac cgccaaccgc     1860

acccugugca ugaaggaggc gaaccggcag aagcgcaaca agucccccag cgaggcccgg     1920

cgcgacggca ugcggaucug ggcccucgag gacgacaacc ugcgccacau ccccgaguuc     1980

aagcgguggc gcuucgccga ggacgccaug gcgcgguucg agggcgagcg cgacuucgag     2040

gcccgggccc ugguggacac gcaguaccuc ucccgcauca gcaaggccua ccuggagacc     2100

cuguacgacg gggcggacgg caagucccac gucugggugg ugccggggcg gcucaccgag     2160

augcugcgcc gccacugggg ccugaacggg auccucaccg acccccugcg ggacgucacg     2220

aaggagaaga accgcaugga ccaccggcac cacgccaucg acgccgccgu gguggccgcg     2280

accgaccgcg gccugaucaa ggccauggcc gagcaggccc ggaccgacgu cgaccagggc     2340

cgcgaggccc ucgcgagcuc caucaugccg cccuggcccg gguuccggga ggccguggcc     2400

gcccaggugg accgcaucau cgucagccac cggcccgacc acggccgcau cgaccugacc     2460

acccggauga aggggcgcga ccagacgacc ggcccccugc accgggacac cgccuacggg     2520

cucaccggcc aguccgagaa gggcgugccc cuggugguca cgcgcgagcc guucgacaac     2580

cugaccccca ccaugaucgg gaagauccgg gacgagcccc ucaaggcggc ccuggccgcc     2640

gugaccgccg gcaagaagga cgacgcguuc gccgaggccc uggcccacuu ccgcgaccgg     2700

cccgggccgu accacggcau ccgccgggug cgccucaucg agcccaucag cgucguggag     2760

auccgggacg cguccgggcg cgccuacaag ggcuacaagg gcaacagcaa ccacugcuuc     2820

gagguguggc gccugcccgg gggcgccauc acggagcaca ucgucuccac cuucgacgcc     2880

caccagcccg ggccggagcc ccggccccac cccgccgcga agcgccugcu ccggcuguuc     2940

aagagcgaca uggugaagcu ggacgacagc aaguucggcc cggugaucgc caccgucgug     3000

auguucgacc gcaacgggaa gaucucccuc gugccccaca ccgccagcaa cgcccggaag     3060

cgcugcgaga aguacaagga ggaccuguac cugcgguuca cggcccgcac ccucaucgag     3120

gcgggcgccc ggcgcaucau cgucgacgag accggccacu uccgggaccc cgggcgcaag     3180

ggcuga                                                                3186


<210>  1503
<211>  3189
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1503
auggccgaga agcagcaccg cuggggccug gacaucggga ccaacagcau cggcugggcc       60

gugaucgcgc ucaucgaggg gcggcccgcc ggccuggucg ccaccggguc ccgcaucuuc      120

agcgacggcc ggaacccgaa ggacggcucc agccuggccg uggagcgccg ggggccccgc      180

cagaugcggc gccggcgcga ccgguaccuc cgccggcgcg accgguucau gcaggcccug      240

aucaacgugg gccugaugcc cggggacgcg gccgcccgca aggcccucgu cacggagaac      300

cccuacgugc ugcggcagcg cggccuggac caggcgcuca cccugccgga guucgggcgg      360

gcccuguucc accucaacca gcgccggggc uuccagucca accgcaagac cgaccgcgcc      420

accgccaagg agagcggcaa ggugaagaac gccaucgcgg ccuuccgggc cgggaugggc      480

aacgcccgca cggucgggga ggcccuggcg cggcgccugg aggacggccg gcccgugcgc      540

gcccggaugg ugggccaggg gaaggacgag cacuacgagc ucuacaucgc ccgcgagugg      600

aucgcccagg aguucgacgc ccugugggcg ucccagcagc gguuccacgc cgagguccug      660

gccgacgccg cccgcgaccg gcuccgcgcg auccugcugu uccagcggaa gcuccugccc      720

gugcccgugg gcaagugcuu ccuggagccg aaccagcccc gcgucgccgc cgcccucccc      780

agcgcgcagc gguuccgccu gaugcaggag cugaaccacc uccgggugau gacccuggcc      840

gacaagcgcg agcggccccu guccuuccag gagcgcaacg accuccuggc ccagcuggug      900

gcccgcccga agugcggguu cgacaugcuc cggaagaucg ucuucggcgc caacaaggag      960

gcguaccgcu ucaccaucga gagcgagcgg cgcaaggagc ugaaggggug cgacaccgcc     1020

gccaagcugg ccaaggugaa cgcccucggc acgcgguggc aggcgcuguc ccuggacgag     1080

caggaccgcc ucgugugccu gcugcucgac ggcgagaacg acgccguccu ggccgacgcc     1140

cugcgggagc acuacgggcu caccgacgcc cagaucgaca cccugcuggg ccucagcuuc     1200

gaggacgggc acaugcgccu gggccggucc gcgcugcugc gcgugcucga cgcccuggag     1260

agcgggcggg acgagcaggg ccugccccuc uccuacgaca aggccguggu cgccgcgggc     1320

uaccccgccc acaccgccga ccuggagaac ggggagcgcg acgcccugcc cuacuacggc     1380

gagcuccugu ggcgguacac gcaggacgcc ccgaccgcga agaacgacgc cgagcgcaag     1440

uucgggaaga ucgccaaccc caccgugcac aucggccuga accagcuccg gaagcuggug     1500

aacgcccuga uccagcgcua cgggaagccc gcccagaucg ucguggagcu cgcgcggaac     1560

cugaaggccg gccuggagga gaaggagcgc aucaagaagc agcagaccgc caaccucgag     1620

cggaacgagc gcauccggca gaagcugcag gacgccggcg uccccgacaa ccgcgagaac     1680

cgccugcgga ugcgccucuu cgaggagcug gggcagggca acgggcuggg cacgccgugc     1740

aucuacagcg gccggcagau cucccuccag cgccuguuca gcaacgacgu ccagguggac     1800

cacauccugc ccuucuccaa gacccucgac gacagcuucg ccaacaaggu gcuggcgcag     1860

cacgacgcca accgguacaa ggggaaccgc ggccccuucg aggccuucgg ggccaaccgg     1920

gacggcuacg cgugggacga cauccgcgcc cgggccgccg ugcugccccg caacaagcgg     1980

aaccgcuucg ccgagaccgc gaugcaggac uggcuccaca acgagaccga cuuccuggcc     2040

cggcagcuga cggacaccgc cuaccucagc cgcguggccc ggcaguaccu gaccgccauc     2100

ugcuccaagg acgacgucua cgugagcccg gggcgccuga ccgcgaugcu ccgggccaag     2160

uggggccuga accgcgugcu ggacggcguc auggaggagc aggggcggcc cgccgugaag     2220

aaccgcgacg accaccgcca ccacgccauc gacgccgugg ucaucggcgc gacggaccgg     2280

gccaugcucc agcaggucgc cacccuggcc gcccgcgcgc gggagcagga cgccgagcgc     2340

cugaucgggg acaugcccac ccccuggccg aacuuccucg aggacgugcg ggccgccgug     2400

gcgcgcugcg ugguguccca caagcccgac cacggccccg agggcgggcu gcacaacgac     2460

accgccuacg gcaucgucgc cgggccguuc gaggacggcc gguaccgcgu gcggcaccgc     2520

gugagccugu ucgaccucaa gcccggggac cuguccaacg uccggugcga cgcgccccug     2580

caggccgagc ucgagcccau cuucgagcag gacgacgccc gcgcccggga gguggcccug     2640

acggcccugg ccgagcgcua ccggcagcgc aagguguggc ucgaggagcu gaugagcguc     2700

cugccgaucc ggccccgcgg cgaggacggg aagacccucc ccgacuccgc gcccuacaag     2760

gccuacaagg gcgacagcaa cuacugcuac gagcuguuca ucaacgagcg gggccgcugg     2820

gacggggagc ugaucuccac cuuccgggcc aaccaggccg ccuaccgccg cuuccggaac     2880

gacccggcgc gcuuccggcg cuacaccgcc ggcgggcggc cccuccugau gcgccugugc     2940

aucaacgacu acaucgccgu gggcacggcc gcggagcgga ccaucuuccg cguggucaag     3000

augagcgaga acaagaucac ccucgccgag cacuucgagg gcgggacccu gaagcagcgg     3060

gacgccgaca aggacgaccc cuucaaguac cugacgaagu cccccggcgc ccuccgcgac     3120

cugggggccc ggcgcaucuu cguggaccug aucggccggg ugcucgaccc ggggaucaag     3180

ggcgacuga                                                             3189


<210>  1504
<211>  3189
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1504
augaaccuga acuacauccu cggccuggac cuggggaucg ccagcguggg cugggccguc       60

guggagaucg acgagcagga gaacccccag cgccucaucg acgugggggu ccggaccuuc      120

gaccgcgcgg aggugcugaa gaccggcgag ucccuggccc ucgcccggcg ccuggcccgg      180

agcucccgcc ggcuggugaa gcgccgggcc gagcgcauca agaaggcgaa gcggcuccug      240

aaggccgaga accugcuccu gagcgccgac gagauccugc cgaacgaggc cuggcagcuc      300

cgcgucaagg ggcuggacca gaagcuggag cggcaggagu gggcggccgu gcuccugcac      360

cuccugaagc accgcggcua ccugucccag cggaagaacg agagcaaguc cgagaacaag      420

gagcugggcg cccuccugag cgggguggcc aucaaccacc agauccugca guccgccgag      480

uaccgcacgc ccgcggagau cgccgucaac aaguuccagg ccgaggaggg ccacauccgg      540

aaccagcgcg gggccuacac ccacaccuuc agccggcucg accugcuggc cgagauggag      600

cuccuguucc agcgccaggc gacccugggc aacccccaca cguccaccaa gcuccuggag      660

aaccugaccg cccuccugau guggcagaag cccgcccugg ccggggaggc cauccucaag      720

augcugggca agugcaccuu cgagccgacg gaguacaagg cggccaagaa cagcuacucc      780

gccgagcggu ucguguggcu gaccaagcuc aacaaccugc gcauccugga gcagggcgcc      840

gagcgcgccc ucaccgacaa cgagcgguuc gcgcugcugg accagcccua cgagaaggcc      900

aagcucaccu acgcccaggu gcgcacgcug cuggggcuga gcgacgaggc caucuucaag      960

ggccuccggu accaggggga ggacaagaag gcggucgaga ccaagaccac ccugauggag     1020

gugaaggccu accaccagau ccgcaaggcc cuggagggcc ucgaccugaa ggccgagugg     1080

aacgagcuga agaacaaccc cgcccuccug gacgagaucg gcacggcguu cucccuguac     1140

aagaccgacg acgacaucca ccgguaccuc gacgggaagc ugagcgaguc cgugcugaac     1200

gcccuccugg agaaccugaa cuucgacaag uucauccagc ucagccugaa ggcccugcag     1260

cagauccucc cccugaugcu gcagggccag cgcuacgacg aggccgucuc cgccaucuac     1320

ggggaccacu acggcaagaa guccgacgag aucgaccggc uccugccgaa cauccccacc     1380

gacgagaucc gcaaccccgu ggugcugcgg acccucacgc aggcgcgcaa ggucaucaac     1440

gccguggugc ggcuguacgg gagccccgcc cgcauccaca ucgagaccgg ccgggagguc     1500

ggcaagagcu accaggaccg caagaagcug gagaagcagc aggaggagaa ccggaagcag     1560

cgcgagaacg ccgugaagaa guucaaggag uacuucccga acuucguggg ggagcccaag     1620

ggcaaggaca uccucaagau gcggcuguac gagcugcagc aggccaagug ccucuacagc     1680

gggaaguccc uggagcugua ccgccuccug gagaagggcu acgucgaggu ggaccacgcg     1740

cugcccuuca gccggaccug ggacgacucc uucaacaaca agguccucgu gcuggccaac     1800

gagaaccaga acaaggggaa cgccaccccc uacgaguggc uggacggcaa gaacaacagc     1860

gagggcuggc agcacuucgu ggcccgcguc cagacguccg gguucagcua cgcgaagaag     1920

cagcgcgugc ucauccagga gcuggacgag aaguccuuca ucgagcggaa ccugaacgac     1980

acccgcuacg uggcccgguu ccucugcaac cacaucgccg acaacaugcu gcugaccggc     2040

aaggggaagc gcaaggucuu cgccagcaac ggccagauca ccgcccuccu gcgggggugc     2100

uggggccugc agaaggugcg cgagaacaac gaccggcacc acgcgcucga cgccgugguc     2160

guggccugcu ccaccaagag caugcaggac aagaucacga aguucgugaa guacaaggag     2220

ggccgccugu ucaaggacaa gaaggacggg gucuacaagg gcgagcggau caaccgcgag     2280

accggggaga ucaucucccu gcacuucccg acccccugga aguucuucaa ggagaacgug     2340

gagauccgga ucuucagcga gaaccccaag cucgagcugg agaaccgccu gcccgacuac     2400

ccgcaguaca accacgaguu cgugcagccc cucuucgucu cccggaugcc cacgcgcaag     2460

augaccggcc agggccacau ggagaccguc aagagcgcca agcggcugga caaggggcug     2520

uccgugcuca aggugccccu gacccagcug aagcucagcg accuggagcg cauggugaac     2580

cgggagcgcg agauggcccu guacgaggcg cucaaggagu gccuggagca guucggcaac     2640

gacccggcca aggccuucgc cgagcccuuc uacaagaagg gcggggcccu ggugaaggcg     2700

guccgggugg agcagacgca gaaguccggg gugcucgucc gcaacggcaa cggcguggcc     2760

gacaacgcca ccgggagcau cgugcggguc gacguguuca ccaagggcgg gaaguacuuc     2820

cuggugccca ucuacaccug gcaggucgcc aagggcaucc ugcccaacaa ggcggugauc     2880

caggggaagg acgagagcga gugggaggag auggacgaga acgccguguu ccaguucucc     2940

augcacaaga acgaccucau caaggucaag accaagaagg gcgagaucuu cggcuacuuc     3000

aacgggcugg accgcgccac gggcagcauc gagaucgagg agcacgaccu ggagaagucc     3060

aaggggaaga acggcagcca ccgcgggcuc ggcgugaaga ccgccuucuu ccuggagaag     3120

uaccaggugg acgagcuggg caagaacauc cggccgugcc gccccaccaa gcggcagcac     3180

guccgcuga                                                             3189


<210>  1505
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1505
auggagaacc acaucaacua ccgccugggc cucgacaucg ggaucgccag cgugggcugg       60

uccgucauca accuggacaa gaagcggauc gaggaccugg gggugcgcau cuucaacgcc      120

gcggagcacc ccaaggacgg cagcucccuc gcccugccgc ggcgcaucgc ccgggggcgc      180

cggcgccugc uccggcgcaa ggccuaccgg gugaagcgcg uccggaagcu gaucguggac      240

uacaacaucc ugaccaagga ccagcucaac gaccuguuca agcagaagug ccagaacagc      300

gugugggaga uccgcgucaa ggcccuggac aacaagguga ccaacgagga gugggcgcgg      360

cuccugauca accugugcaa gcgccggggc uucaagucca accgcaagaa cgaggccgac      420

gacaaggaga cgaagaaggu gaucaacagc aucaagucca acaucaaggg caugaaggag      480

agcggguccc ggaccaucgg cgaguacauc uacacccaga cccucagcuc cggggacauc      540

agcaaguccu uccgcaacaa ggacggccag uacaacaacu gcgucagccg gaagaugauc      600

gaggaggaga ucaagauccu guucaagaag cagcgcgagc uggggugcga guucgccucc      660

gaggagaucg aggagaagua ccucgccauc uucaacagcc agcgccccuu cuccaaguuc      720

gaggaccugg agaagauggu gggcuucugc acguucgaga aggcgagcaa ggagcggcgc      780

gcccccaagu gcuccaucag cgccgaggag uucgugcugu acgacaacau gaacaagcuc      840

agcauccuga acaagggcga cacccggaag cugaccaacg aggagcgcaa gaucaucguc      900

gagaacgccu ucaagaacaa ggagaucaag uacuccaagc uccggaagau ccugaaccug      960

agcaacgacg agcgcuucuc cacccucagc uacuccaaca agacggagcu gagcaagacc     1020

gagaacucca aguucguggc ccugaagggg uaccacgaga ucaagagcgc gaucgagaag     1080

uccuucggca aggacaccug gaaccgggug aagaacaacc gcaccaugcu caacgacauc     1140

gccuacgucc ugagcaucgc caagacggac gaggacauca ucaagcagcu gucccuccgg     1200

aacauccccc gcgagaucau cgacgccgug cuggggcuga gcuucuccaa guucagcaac     1260

cucuccauca aggccaucga gaagauccug ccguaccuga aggacggcca gcaguacaac     1320

gaggcgugca agagcgccgg cuacgacuuc aaggccaccu acaacgggga gaaguccuac     1380

aagcuccccc ugaucgacau cgacgagauc gugaaccccg ucgugaaccg ggcccugagc     1440

cagacccgca aggugaucaa cgccgucauc gaccgguacg gcucccccau cgggaucaac     1500

aucgagcucg cgcgcgaccu ggccaagaac uucaaggacc ggagcaagau caagaaggag     1560

caggaggaga accgcgccaa caaggagaag aucaagaacg agcugaagau gcucaugaac     1620

aaggagccga ccagcuccga gauccugaag uaccggcugu ggaaggagca gcgcgaggag     1680

ugcgccuaca gccaggagcg gaucuacauc gaggaccucu ucaagguggg ccaguacgag     1740

aucgaccaca ucauccccuu cucccgcagc uucgacgacu cccugaacaa caaggugcug     1800

gucaaggccg cggagaacca gcgcaaggcc aaccggaucc ccuacgagua cuucgggagc     1860

gacgaggacc gcugguaccg guucgaggug ugggucgagc aguccagccu guccuucaag     1920

aagaagaaca accuccugaa gaagcgcuuc agccgggagg acuccaucga guucaaggac     1980

cgcaaccugg uggacacgca guacaucagc cgguacaucu ccaacuucau caagagccgc     2040

cucaucuuca aggaguccga cagcaagcag aaggugauca ccaucaacgg ccgggccacc     2100

uccuaccugc gcgccaagug gggccugcuc aagguccggg agaacgggga caagcaccac     2160

gcgcuggacg ccaccgugau cgccguggcc acgcagggca ugguccagga gaucagcaag     2220

uacagcaagg cccacgagcu gaaguacauc cgccaggggg acgaguucau cgacaucgag     2280

accggcgaga ucgugaaggu gaaggaccac aagcaccucu ccaaggaccg gcugcccgag     2340

ccguggcugg gguucagcuc cgagcuccgc cugcggcuga gcgagaaccc caucgaggag     2400

cucaagaagu cccccaucag caccuacgac gaggaguuca uccgcaagac cguccggccc     2460

aucuucgugu cccgcgugcc guaccggaag gucacgggcc gccuguucga ggagaccgug     2520

uacagcccca aggcguucau cgacgagggc uucaucgcca agaagaagcu gcaggaccuc     2580

acccugaagg acuucgagaa caucuacaac uacaacuccg acaagaagcu guacgacgcc     2640

cuccgggagc gccuggagga guacgggggc aacgccaaga aggccuucgc ggaggaguuc     2700

cgcaagccca ccaagacggg gaagcugggc cccaucguca agagcaucaa ggugaagacc     2760

aagaucccgu ucaaggacgg gaucgaccuc aacggcggca aggugaagaa ggacgggcug     2820

gugcggaucg acaucuacga gaaggagggc aaguacuacg ucgugcccgu guaccgccug     2880

gacauccagc aggggaacau ccccaagaag gccgccgucg ccaacaagga ggagaaggac     2940

uggauccuca uggacgaguc cuacaacuuc aaguucagcc uguacaagaa cgaccugauc     3000

uacaucaagu acgacuccaa gggcgaguac uucggcuacu acgacggguu cgaccggcac     3060

accggcgcca ucacccucga gcgccacgac aacagcgagc gguaccgcgg gaucggcauc     3120

aagacggggg ugaaggagau cuucaaguac aacguggacg uccugggccg guacuacaag     3180

guggcgaagu ga                                                         3192


<210>  1506
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1506
auggagaagu uccacuacgu gcugggccuc gaccugggga ucgccagcgu cggcugggcc       60

gcgaucgaga ucgacaagga gaccgagacc uccaucgggc ugcucgacug cggcgugcgc      120

acguucgagc gggccgaggu gcccaagacc ggggacagcc uggccaaggc ccgccgggag      180

gcccgcucca cccggcgccu gauccggcgc cggagccacc gccuccugcg gcugaagcgc      240

cuccugaagc gggagaucuu ccgccagccg gagaccuuca aggaccugcc caucaacgcg      300

uggcagcucc gggucaaggg ccuggacucc cgccugaacg aguacgagug ggccgccgug      360

cuccugcacc uggugaagca ccggggcuac cucagccagc gcaaguccga gaugagcgag      420

acggacucca agagcgagau ggggcggcug cuggccggcg ucgcggagaa ccaccagcuc      480

cugcagcagg agcaguaccg cacccccgcc gagcuggccc ucaagaaguu cgugaagcac      540

uuccggaaca agggcgggga cuacgcccac accuucaacc gccuggaccu gcaggccgag      600

cuccaccucc uguuccagaa gcagcgcgag cuggggaacc ccuucaccuc cccggagcug      660

gagcgccagg uggacgaccu gcucaugacg cagcggagcg cgcugcaggg cgacgccauc      720

cugaagaugc ucggccacug cggguucgag cccgagcagu ucaaggccgc caagaacacc      780

uucuccgccg agcgguucau cuggcugacc aagcugaaca accuccgcau ccaggaccag      840

ggcaaggagc gggcgcugac cgccgacgag cgcacgaagc ugcucgacga gcccuacaag      900

aaguccaagc ugaccuacgc ccagguccgg aagcugcuca gccugcccca gaccgccauc      960

uucaaggggc ugcgcuacga ccucgagcac gacaagaagg ccgagaacag cacccugaug     1020

gagaugaagu ccuaccacaa cauccggcag acgcuggaga aguccggccu gaagaccgag     1080

uggcagagca ucgcgaccca gccggagauc cucgacgcca ucggcaccgc cuucagcauc     1140

uacaagacgg acgaggacau cagccacgag cugaagaccu gccgccugcc cgagaacgug     1200

cucaacgagc ugcugaagaa caucaacuuc gacggguuca uccagcucuc ccugaccgcc     1260

cugcggaaga uccucccccu gauggagcag ggcuaccgcu acgacgaggc cugcacccag     1320

aucuacggga accaccacag cggcucccug cagcaggaga gcaagcaguu ccucccccac     1380

aucccgaucg acgacgugcg gaaccccguc guguuccgca cgcugaccca ggcgcggaag     1440

guggucaacg ccaucauccg ccgguacggg ucccccgccc gcgugcacau cgagauggcc     1500

cgggagcugg gcaagagcaa guccgaccgc gaccgcaucg agaagcagca gcagaagaac     1560

aagaaggagc gggagaacgc gguggccaag uucaaggagg acuuccccga cuucgucggc     1620

gagccgcgcg ggaaggacau ccucaagaug cggcuguacg agcagcagca cggcaagugc     1680

cuguacagcg ggcacgacau cgacaucaac cgccucaacg agaagggcua cguggagauc     1740

gaccacgccc ugcccuucuc ccggaccugg gacgacagcc agaacaacaa ggugcugguc     1800

cucggguccg agaaccagaa caagcgcaac cagacccccg acgaguaccu ggacggcgcc     1860

aacaacagcc agcgguggcu ggaguuccag gcccgcgugc agacgugcca cuucuccuac     1920

ggcaagaagc agcggaucca gcucgcgaag cuggacgacg agaccgagaa gggguuccug     1980

gagcgcaacc ucaacgacac ccgguacauc gcccgcuuca ugugccaguu cgugcaggag     2040

aaccuguacc ugaccggcaa ggggaagcgg cucgucuucg ccagcaacgg cgggaugacg     2100

gccacccugc gcaaccugug gggccuccgg aaggugcgcg aggacaacga ccggcaccac     2160

gcccuggacg cgaucgucgu ggccugcucc accgccagca ugcagcagaa gaucaccaag     2220

gccuuccagc gccacgaguc caucgaguac guggacacgg agaccggcga ggucaaguuc     2280

cggauccccc agccguggga cuucuuccgc caggagguga ugauccgggu guucagcgac     2340

cagcccugcg aggaccuggu cgagaagcuc uccgcccgcc ccgaggcgcu gcacgacaac     2400

gugacgcccc uguucgugag ccgcgccccg aaccggaaga ugagcgggca gggccaccuc     2460

gagaccauca aguccgccaa gcgccugagc gaggagaacu ccauggucaa gaagccccug     2520

accacccuca agcugaagga cauccccgag aucguggggu accccagccg ggagccgcag     2580

cuguacgccg cgcucaagac ccgccuggag acccacgacg acgaccccau caaggccuuc     2640

gccaagcccu ucuacaagcc gaacaagaac ggcgagcugg gcgcccucgu gcgguccguc     2700

cgcgugaagg gggugcagaa caccggcguc auggugcacg acgggaaggg caucgccgac     2760

aacgcgacga uggugcgggu cgacguguac accaaggccg ggaagaacua ccuggugccc     2820

gucuacgugu ggcaggucgc ccagggcauc cugcccaacc gcgccgugac cagcggcaag     2880

uccgaggccg acugggaccu caucgacgag agcuucgagu ucaaguucuc ccugagccgc     2940

ggggaccugg uggagaugau cuccaacaag ggccggaucu ucggguacua caacggccuc     3000

gaccgggcga acgggagcau cggcauccgc gagcacgacc uggagaaguc caagggcaag     3060

gacggggugc accgggucgg cgugaagacc gccacggccu ucaacaagua ccacguggac     3120

ccccugggga aggagaucca ccgcugcagc uccgagccgc ggcccacccu caagaucaag     3180

agcaagaagu ga                                                         3192


<210>  1507
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1507
auggagaagg acuacauccu gggccucgac aucgggaucg gcagcguggg guacggccug       60

aucgacuacg acaccaaguc caucaucgac gccggggucc gccuguuccc cgaggccaac      120

gcggacaaca accucggccg gcgcgccaag cggggcgccc gccggcugaa gcgccggcgc      180

auccaccggc uggagcgcgu caagagccuc cuguccgagu acaagaucau cagcgggcug      240

gccccgacca acaaccagcc cuacaacauc cgggugaagg gccucacgga gcagcugacc      300

aaggacgagc uggccguggc gcuccugcac aucgccaagc gccgggggau ccacaacgug      360

gacguggccg ccgacaagga ggagaccgcg uccgacagcc uguccaccaa ggaccagauc      420

aacaagaacg ccaaguuccu cgagagccgc uacgucugcg agcugcagaa ggagcggcug      480

gagaacgagg gccacgugcg cgggguggag aaccgguucc ucacgaagga caucguccgc      540

gaggccaaga agaucaucga cacccagaug caguacuacc ccgagaucga cgagaccuuc      600

aaggagaagu acaucucccu gguggagacc cggcgcgagu acuacgaggg ccccggcaag      660

gggagcccgu acggcuggga cgccgacgug aagaaguggu accagcugau gauggggcac      720

ugcacguacu uccccgucga guuccggucc gugaaguacg ccuacaccgc ggaccucuac      780

aacgcccuga acgaccugaa caaccucacc aucgcccgcg acgacaaccc caagcuggag      840

uaccacgaga aguaccacau caucgagaac guguucaagc agaagcgcaa ccccacccug      900

aagcagaucg ccaaggagau cggcgucaac gacaucaaca ucagcggcua ccgggugacg      960

aaguccggga agccgcaguu caccagcuuc aagcuguucc acgaccucaa gaaggugguc     1020

aaggaccacg ccauccugga cgacaucgac cuccugaacc agaucgcgga gauccugacc     1080

aucuaccagg acaaggacuc caucguggcc gagcucggcc agcuggagua ccugaugagc     1140

gaggccgaca agcaguccau cagcgagcuc accggguaca cgggcaccca cucccugagc     1200

cugaagugca ugaacaugau caucgacgag cucuggcaca gcuccaugaa ccagauggag     1260

guguucaccu accugaacau gcgccccaag aaguacgagc ugaaggggua ccagcggauc     1320

cccaccgaca ugaucgacga cgccauccuc agccccgucg ugaagcgcuc cuucaagcag     1380

gccaucggcg uggucaacgc gaucaucaag aaguacggcc ugccgaagga caucaucauc     1440

gagcuggccc gggagagcaa cuccgccgag aagucccgcu accuccgggc cauccagaag     1500

aagaacgaga agacgcgcga gcggaucgag gccaucauca aggaguacgg gaacgagaac     1560

gcgaagggcc uggugcagaa gaucaagcug cacgacgccc aggaggggaa gugccucuac     1620

agccugaagg acaucccccu ggaggaccug cuccgcaacc ccaacaacua cgacaucgac     1680

cacaucaucc cccggagcgu cuccuucgac gacagcaugc acaacaaggu gcuggugcgc     1740

cgggagcaga acgccaagaa gaacaaccag accccguacc aguaccugac cuccggcuac     1800

gccgacauca aguacagcgu cuucaagcag cacgugcuca accuggcgga gaacaaggac     1860

cgcaugacca agaagaagcg ggaguaccug cucgaggagc gcaacaucaa caaguacgac     1920

gugcagaagg aguucaucaa ccggaaccug gucgacacgc gcuacaccac ccgggagcug     1980

accacgcucc ugaagaccua cuucaccauc aacaaccugg acgugaaggu gaagaccauc     2040

aacggguccu ucacggacuu ccuccgcaag cgcuggggcu ucaagaagaa ccgggacgag     2100

ggcuacaagc accacgccga ggacgcccug aucaucgcca acgccgacua ccuguucaag     2160

gagcacaagc uccugaagga gaucaaggac gucagcgacc uggcggggga cgagcgcaac     2220

uccaacguga aggacgagga ccaguacgag gagguguucg gcggguacuu caagaucgag     2280

gacaucaaga aguacaagau caagaaguuc agccaccggg ucgacaagaa gcccaaccgc     2340

cagcucauca acgacaccau cuacuccacc cgggugaagg acgacaagcg cuaccugauc     2400

aacacccuga agaaccucua cgacaagagc aacggcgacc ugaaggagcg gaugcagaag     2460

gaccccgagu cccugcucau guaccaccac gacccccaga cguucgagaa gcugaagauc     2520

gugaugagcc aguacgagaa cgagaagaac ccgcuggcca aguacuucga ggagaccggg     2580

caguaccuca ccaaguacgc caagcacgac aacggccccg ccauccacaa gaucaaguac     2640

uacggcaaca agcuggucga gcaccuggac aucaccaaga acuaccacaa cccccagaac     2700

aagguggugc agcucagcca gaaguccuuc cgcuucgacg ucuaccagac cgacaagggg     2760

uacaaguuca ucagcaucgc cuaccugacg cugaagaacg agaagaacua cuacgcgauc     2820

ucccaggaga aguacgacca gcucaagagc gagaagaaga ucuccaacaa cgccguguuc     2880

aucggcagcu ucuacaccuc cgacaucauc gagaucaaca acgagaaguu ccgggugauc     2940

ggggucaaca gcgacaagaa caaccugauc gagguggacc gcaucgacau ccggcagaag     3000

gaguucaucg agcuggagga ggagaagcag gucaacuaca ugaacuccga cagcuaccuc     3060

aagaacaacc gcaucaaggu gaccaucggc cggaagacca cgaacaucga gaaguuccac     3120

accgacaucc ugggcaacau guacaagucc aagcgcccca aggccccgca gcugguguuc     3180

aagaaggggu ga                                                         3192


<210>  1508
<211>  3195
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1508
augagcgagu gcgugacccg ccggauccug ggcaucgacc ucgggaucgc cuccugcggc       60

uggggcguca ucgagguggg ggaggccagc ggguccauca ucgcgagcgg cgugcgcugc      120

uucgacgcgc cccugaucga caagaccggc gagccgaagu ccgccacgcg gcgcaccgcc      180

cgggggcagc gccggaucau ccgccggcgc cggcagcgca ugaacgccgu ccggcgccug      240

cucgccgagu ucggcgugcu gaccgggcgg agccccgacg cccugcacca ggcccuccug      300

cgccuguccc agagcguggc cggcucccag gucacccccu ggacgcuccg ggcggccgcc      360

cacgagcgca agcugaccaa cgacgagcug gccguggugc ucgggcacau cgcccggcac      420

cgcggcuucc ggagcaacuc caagaacgac ggcggggcga acgccgccga cgagaccagc      480

aagaugaaga aggccaugga gaccacgcgc gagggccugg cccgcuacca cuccuucggg      540

gcgaugaucg ccagcgaccc caaguucgcc gaccggaagc gcaaccggga caaggacuac      600

ucccacaccg ccaagcgcag cgaccuggag gacgaggucc ggaccaucuu ccgcucccag      660

acccgguucg gcagccucgu ggccuccgag aagcugagcc aggcguucgc cgacgccgcc      720

uucuuccagc gcccgcugca ggacagcgag gacauggugg gcuccugccc cuucgagccc      780

gggcagaagc ggacggcccg ccgggcgccc agcuucgagc uguuccgcuu ccucucccgg      840

cuggccaacc ucaagcugac cgucggccgc gccccggagc ggcgccugac ccccgacgag      900

aucgcccucg cggccaaggg guucggcgag accaagaagu ccaucacguu caagagccug      960

cgggaggccc uggaccucga ccccaacgcc cgcuucagcg ggguggccaa ggagaaggag     1020

uccacccugg acguggcggc ccggaccggc ggcgccgccu acgggaccaa gacgcugaag     1080

gacgcccucg gcgacgcgcc cuggcgcagc cugucccgca ugccggagaa gcuggaccgg     1140

aucgccgaga uccucagcuu ccgcgaggac augaaggcca uccggaacgg gcuggaggag     1200

gucggccugg acgggcucgu gguggacgcc cugaugcagg ccaccgcgaa cggcgacuuc     1260

aaggacuuca cccgcgccgc ccacaucucc gcccuggcgg cccggaacau cauccccggc     1320

cuccgcgagg ggcuggucua cagcgacgcc ugcacccggg ugggcuacga ccacgccgcc     1380

cgccccgcgg ugccccuguc ccagaucggg agcccgguca cgcggaaggc ccucuccgag     1440

gcccugaagc agguccgcgc cguggcccgg gaguacggcc ccaucgacua cuuccacauc     1500

gagcuggcgc gcagcaucgg gaaguccgcc gaggagcgga agaagcucac cgacggcauc     1560

gaggcccgca acguggagaa ggagaagcgg cgcaaggagg ccgccgagca ccugggccgg     1620

gcgcccagcg acgacgagcu gcuccgcuac gagcuggcca aggagcagaa cuucaagugc     1680

aucuacuccg gggaccccau cgacccggcc ggcaucagcg ccaacgacac ccgguaccag     1740

guggaccaca uccugcccug gagccgcuuc ggggacgacu ccuacgucaa caagacccug     1800

ugcacggcgc ggagcaacca gaacaagcgc ggccgcaccc ccuucgagug guucgacgcc     1860

gacaagaccg aggccgagug gauggaguac uccgcccggg uggaggaccu caaggaggug     1920

aagggccgca agaagcggaa cuacagcauc aaggacgccg cguccgucga ggacaaguuc     1980

aaggcccgca accugaccga cacgcagugg gccacccggc ugcucgccga cgagcugaag     2040

cgcauguucc cgccccggga gugcgagcgc guggugaccg uccgggccga cgggggcaac     2100

gacgggcuga gcaucgugga ggagcgccgg guguucaccc gccccggcgc gaucacgucc     2160

aagcuccggc gcgccugggg gcuggagggc cugaagaagc aggacggcaa gcgggucgag     2220

gacgaccgcc accacgccgu ggacgcccuc gugcuggccg cgaccaccga gagccugcuc     2280

aaccggcuga ccgucgaggu gcagcagcgc gagcgggagg ggcgccagga cgacaucuuc     2340

cacugcuccc agcccuggcc cggcuuccgc guggacgucc agcggaccgu guacgggagc     2400

gagacgaugc cgggcaucuu cgugucccgc gccgagcggc gccgggcccg cgggaaggcc     2460

cacgacgcca ccgucaagca gauccgggac aucgacggcg agcgcaucgu guucgagcgg     2520

aagcccaucg agaagcugac cgacaaggac cucgagcgca uccccgugcc ggagcccuac     2580

ggcaaggcgg ccgaccccaa gaagcugcgg gacgagcugg ucgagaaccu ccgcgccugg     2640

aucgccgcgg ggaagcccaa ggacaagccg ccccggagcc ccaagggcga caucauccgc     2700

aaggugcgga ucgagaccaa ggacaaggug gccgucgaga ucaacggggg cacgguggac     2760

cgcggggaca uggcccgggu ggacgucuuc cgcaagaaga acaagaaggg cgugugggag     2820

uucuacguga uccccaucua cccgcaccag aucgucgccu ccgcccugcc gcccaaccgg     2880

gcggugaucg ccuacaaggc cgagagcgag uggaccgcca ucgacggcug cuucgaguuc     2940

gccugguccc ugaaccccau gagcuaccuc gagcugguga aguccaacgg ggagcugauc     3000

gagggcuacu uccgcagcau ggaccggacc accggggcga ucaaccucag ccccaugucc     3060

acgaacagcg agaccauccg cuccaucggc gucaagaccc ugagcuccuu ccgcaaguuc     3120

accguggacc ggcuggggcg caaguucgag aucccccggg aggugcgcac guggcggggc     3180

gaggccugca ccuga                                                      3195


<210>  1509
<211>  3195
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1509
augaagcgca ccagccugcg ggccuaccgc cucggcgugg accugggggc caacucccug       60

ggcugguucg ucguguggcu cgacgaccac gggcagcccg agggccuggg gccgggcggc      120

gugcggaucu uccccgacgg gcgcaacccc cagagcaagc aguccaacgc ggccggccgg      180

cgccuggccc ggagcgcccg ccggcgccgg gaccgcuacc uccagcggcg cgggaagcug      240

augggccugc ucgucaagca cgggcugaug cccgccgacg agccggcgcg gaagcgccug      300

gagugccucg accccuacgg ccugcgggcc aaggcccugg acgaggugcu cccccugcac      360

cacgugggcc gcgcccuguu ccaccucaac cagcggcgcg ggcuguucgc gaaccgggcc      420

aucgagcagg gcgacaagga cgccuccgcc aucaaggccg cggccgggcg ccugcagacc      480

agcaugcagg ccugcggcgc ccgcacgcuc ggcgaguucc ugaaccggcg ccaccagcug      540

cgggccaccg uccgcgcgcg gucccccguc gggggcgacg ugcaggcccg cuacgaguuc      600

uacccgaccc gggccauggu ggacgccgag uucgaggcca ucugggcggc ccaggcgccc      660

caccacccca ccaugacggc cgaggcccac gacaccaucc gcgaggccau cuucagccag      720

cgggccauga agcgccccuc caucgggaag ugcagccucg acccggccac cucccaggac      780

gacguggacg gcuuccggug cgccuggagc cacccccugg cgcagcgcuu ccggaucugg      840

caggacgugc gcaaccuggc cgucguggag accgggccca cguccagccg gcucggcaag      900

gaggaccagg acaagguggc ccgcgcccug cugcagaccg accagcucuc cuucgacgag      960

auccggggcc ugcuggggcu ccccagcgac gcccgcuuca accuggaguc cgaccggcgc     1020

gaccaccuga agggcgacgc gaccggggcc auccucagcg cccgccggca cuucggcccg     1080

gccuggcacg accgcucccu ggaccggcag aucgacaucg ucgcccugcu cgagagcgcg     1140

cuggacgagg ccgccaucau cgccagccug gggaccacgc acucccucga cgaggccgcg     1200

gcccagcgcg cccugagcgc ccugcucccc gacggcuacu gccggcuggg ccugcgcgcg     1260

aucaagcggg ugcucccccu gauggaggcc gggcgcaccu acgccgaggc cgccuccgcg     1320

gccggcuacg accacgcccu gcugcccggg ggcaagcuca gcccgaccgg guaccugccc     1380

uacuacggcc aguggcugca gaacgacgug gucggcuccg acgacgagcg ggacaccaac     1440

gagcgccggu gggggcgccu ccccaacccc acggugcaca ucggcaucgg gcagcugcgg     1500

cgcgugguca acgagcugau ccgguggcac ggcccgcccg ccgagaucac cguggagcuc     1560

acccgcgacc ugaagcugag cccccggcgc cucgccgagc uggagcggga gcaggcggag     1620

aaccagcgca agaacgacaa gcggaccucc cugcuccgca agcugggccu gcccgccagc     1680

acgcacaacc uccugaagcu gcgccucugg gacgagcagg gggacguggc cuccgagugc     1740

ccguacaccg gcgaggccau cgggcuggag cggcugguca gcgacgacgu ggacaucgac     1800

caccucaucc ccuucuccau cagcugggac gacuccgccg cgaacaaggu cgugugcaug     1860

cgcuacgcca accgggagaa gggcaaccgc acccccuucg aggccuucgg gcaccggcag     1920

ggccgcccgu acgacugggc cgacaucgcg gagcgggccg cccgccugcc ccggggcaag     1980

cgcuggcggu ucgggcccgg cgcccgcgcc caguucgagg agcuggggga cuuccaggcg     2040

cggcuccuga acgagaccag cuggcuggcc cgcguggcca agcaguaccu cgccgccguc     2100

acgcaccccc accggaucca cgugcugccg ggccgccuga ccgcgcuccu gcgggccacc     2160

ugggagcuga acgaccuccu gcccgggucc gacgaccgcg ccgccaagag ccggaaggac     2220

caccgccacc acgccaucga cgcgcuggug gccgcccuca ccgaccaggc ccugcugcgc     2280

cggauggcca acgcgcacga cgacacgcgc cggaagaucg agguccuccu gcccuggccc     2340

accuuccgca ucgaccugga gacccggcuc aaggccaugc ugguguccca caagccggac     2400

cacggccugc aggcccgccu ccacgaggac accgccuacg gcacggugga gcaccccgag     2460

accgaggacg gggcgaaccu ggucuaccgg aagaccuucg uggacaucag cgagaaggag     2520

aucgaccgca uccgggaccg ccggcugcgc gaccucgugc gggcccacgu cgccggcgag     2580

cgccagcagg ggaagacccu gaaggccgcc gugcuguccu ucgcgcagcg gcgcgacauc     2640

gccggccacc ccaacgggau ccggcacgug cgccucacca agagcaucaa gcccgacuac     2700

cuggucccga uccgggacaa ggccggccgc aucuacaaga gcuacaacgc cggcgagaac     2760

gccuucgugg acauccugca ggcggagucc gggcggugga ucgcccgcgc cacgaccgug     2820

uuccaggcca accaggccaa cgagagccac gacgcgcccg ccgcccagcc caucaugcgc     2880

gucuucaagg gcgacaugcu ccggaucgac cacgccgggg cggagaaguu cgugaagauc     2940

gugcgccugu cccccagcaa caaccugcuc uaccuggucg agcaccacca ggccggcgug     3000

uuccagaccc ggcacgacga cccggaggac uccuuccgcu ggcuguucgc cagcuucgac     3060

aagcuccggg aguggaacgc cgagcuggug cgcaucgaca cccuggggca gcccuggcgg     3120

cgcaagcggg gccucgagac gggcuccgag gacgccaccc gcaucgggug gacccggccc     3180

aagaaguggc ccuga                                                      3195


<210>  1510
<211>  3198
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1510
augaacaagg acaucaacua cgcccugggc cucgacaucg ggaucaccag cgugggcugg       60

gccgucauca accuggaccu gaaccgcauc gaggaccucg gggugcggau cuucaacgcg      120

gccgagaacc ugaaggacgg cuccagccug gcccuccccc gccggcuggc ccgcgggcgg      180

cgccggcugc uccgccggaa ggccuaccgc guggagcggg uccgcaagcu gauccugaag      240

aacaacaucc ucaccaagga ggagcuggag ggccuguuca agaacaagga ggugaucgac      300

gugugggagg cgcgggucaa gggccucgac uacaagcugu cccgcgagga gugggccaag      360

auccugauca acuucugcaa gcggcgcggg uucaagagca accggaagaa cgaggccaag      420

gacaaggagg ccggccagau ccucuccagc aucuccuaca acaucgagaa gaugaaggag      480

acgaacagcc gcaccaucgg ggaguacauc uacaacgagg ugaagaacuc cgaggacagc      540

uacgcgccgc ugcggaacaa guucggcgag uacaagaugu gcgugucccg cgacgucauc      600

cgggaggaga uccacacccu guucgagaag cagcgcgagc ucgggaacaa cuucgccagc      660

gacgagaucg aggacaagua cuucgagauc uucaacuccc agcgccccuu cagcaacuuc      720

gaggaccugg agaagcuggu gggcuucugc accuucgagc ggaagaagca caagcgcgcc      780

cccaagcacu gcaucuccag cgaggaguuc acgcucuacg agggcaucaa caagcugucc      840

aucaucaagg acggggagaa gcggaagcug agcgaggagg agcgcacccu cguggucaac      900

gaggccuuca acaagaagga gaucaaguac accacccugc ggaagcugcu cggccugucc      960

gaggaggagc uguucagcac gcucaccuac agcaucgaca aggacaucuc caagaccgag     1020

aacaccaagu ucgugagccu gaagggguac cacgagauca agcgcgccau cgagaagggc     1080

guguccaaga aggcguggca ggacaucgag aacaaccgga accugcucaa cgacaucgcc     1140

uacguccuga cgcugggcaa gaccgacgac gagaucgaga agcagcuccg ccugcggaac     1200

gugcccgagg agcuguucga gagccuccug gacauguccu ucaacaaguu caacaaccug     1260

agcaucuacg cccucgagaa gauccugccg uucaugaagg agggguacca guacaacgag     1320

gccugcgaga aggccggcua caacuucaag gcgaucuacg agggggugcg caccaagaag     1380

cugcccguca ucgagaucga cgagaucgug aaccccgugg ucaaccgggc ccucgcccag     1440

acccgcaagg ugaucaacuc cgugaucgac aaguacggca gccccguccg gaucaacauc     1500

gagcuggccc gcgaccuggc caagaacuuc aaggaccgga agcugaucga gaaggagcag     1560

aaggagaacc gcgcgaaccu cgacaagauc cgggagaacc ugcgcgagcu gauggacaag     1620

gagccgacgg uggucgaggu gcucaaguac cggcuguggg agcagcagcg cggggagugc     1680

gccuacaccc agucccagau cccccuggag cgccucuuca gccccggcua cugcgagauc     1740

gaccacauca uccccuucuc ccggagcuuc gacgacuccc ugagcaacaa gauccuggug     1800

cucggcuccg agaaccagcg caaggggaac cggaccccgu acgaguacuu cggcgaggac     1860

agcgagcgcu ggaacuccuu cgagaucugg gucaagggga gcuaccugaa cuacaagaag     1920

aaggccaacc ugcucaagaa gaaguucucc gaggaggagc agcgggacug gaaggcccgc     1980

aaccugcagg acaccaagua caucugccgg uacaucagca acuucgugaa caaccgccug     2040

gaguucaagg agagcgagaa caagcagaag gugaucacgg ucaacggccg ggccaccgcg     2100

uaccuccgcu cccggugggg gcugaacaag gugcgcgagg acggcgacaa gcaccacgcc     2160

cuggacgccg ccguggucgg cgugaccacc caggggaugg ugcagaagau cagcaaguac     2220

uccaaggcgc acgagcucua cacgguccgg aagaacgacg aguucgugga cguggagacc     2280

ggcgagugcg ucgacgugga cgaguacaag gagauccgca aggacauccu gccccggccc     2340

uggcgcgggu ucagcgagga gcugaagcuc cggcuguccg acaacccccu ggaggagcuc     2400

aagaaguccc cgaucgagac cuacaacgag gaguucauca aggagaacgu gaagcccauc     2460

uucgucagcc gcgugcccuu ccggaagauc agcggcaagc uguucaagga gaccaucuac     2520

uccgagcgcg ccuucaagga cggguacuuc gugagcaaga agucccuggu cgaccucaag     2580

cggggcgacc uggacaacuu cuacaacuac gagugcgaca agaagcugua cgacgccauc     2640

agcgagcgca ugaaggaguu caacuacaac ggcaagaagg ccuucgaguc ccccuuccgc     2700

aagccgacca agagcgggaa ggagggcccc guggugcggu ccgucaagau caagagcgcc     2760

gugcccuuca aggacgggau ccccaugaac gagggccucg uggcgaagga gggcaugguc     2820

cgcaucgaca ucuacgagaa ggaggggaag uacuucgugg ugccggucua ccgguaccag     2880

cuggccaagg gcaucgugcc caagcgcgcc gccaucgcca agaaggacga gcugaagugg     2940

cccaucaugg accagacgua caacuucaag uucuccaucu acaagaacga ccucaucgag     3000

gugaaguacg acaagaaggg ggucuacuuc ggcuacuacg acggguucga ccggaacagc     3060

gcgagccuga ccaucgagaa gcacgacaac uccgagcgcu accggagcau cggccugaag     3120

gugggcgugc ucgagaucaa caaguacgag gucgacgugc uggggaacua ccacaaggug     3180

aagcugggcg ggaacuga                                                   3198


<210>  1511
<211>  3198
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1511
augaacaagg acaucaacua cgcccugggc cucgacaucg ggaucaccag cgugggcugg       60

gccgucauca accuggaccu gaaccgcauc gaggaccucg gggugcggau cuucaacgcg      120

gccgagaacc ccaaggacgg cuccagccug gcccugccgc gccggcucgc ccgcgggcgg      180

cgccggcugc ugcgccggaa ggccuaccgc guggagcggg uccgcaagcu caucgugaag      240

aacaacaucc ugaccaagga ggagcuggag ggccucuuca agaacaagga ggugaucgac      300

gucugggagg cgcgggugaa gggccuggac uacaagcugu cccgcgagga gugggccaag      360

auccucauca acuucugcaa gcggcgcggg uucaagagca accggaagaa cgagacgaag      420

gacaaggagg ccggccagau ccuguccagc aucuccuaca acaucgagaa gaugaaggag      480

accaacagcc gcaccaucgg ggaguacauc uacaacgagg ugaagaacuc cgacgacagc      540

uacgcgcccc ugcggaacaa guucggcgag uacaagaugu gcgucucccg cgacgugcuc      600

cgggaggaga uccacacccu guucgagaag cagcgcgagc uggggaacaa cuucgccagc      660

gacgagaucg aggacaagua cuucgagauc uucaacuccc agcgccccuu cagcaacuuc      720

gaggaccucg agaagcuggu gggcuucugc acguucgagc ggaagaagca caagcgcgcc      780

cccaagcacu gcaucuccag cgaggaguuc acccuguacg agggcaucaa caagcucucc      840

aucaucaagg acggggagaa gcggaagcug agcgaggagg agcgcacccu ggucgugaac      900

gaggccuuca acaagaagga gaucaaguac accacgcucc ggaagcugcu gggccucucc      960

gaggacgagc uguucagcac ccugaccuac agcaucgaca aggacaucuc caagaccgag     1020

aacacgaagu ucgugagccu caagggguac cacgagauca agcgcgccau cgagaagggc     1080

gucuccaaga aggccuggca ggacaucgag aacaaccgga accugcugaa cgacaucgcg     1140

uacgugcuca cccugggcaa gaccgacgac gagaucgaga agcagcugcg ccuccggaac     1200

gugccggagg agcuguucga gagccugcuc gacauguccu ucagcaaguu caacaaccug     1260

uccaucuacg cccuggagaa gauccucccc uucaugaagg agggguacca guacaacgag     1320

gccugcgaga aggccggcua caacuucaag gccaucuacg aggggguccg caccaagaag     1380

cugcccguga ucgagaucga cgagaucgug aaccccgucg ugaaccgggc gcuggcccag     1440

acgcgcaagg ugaucaacag cgucaucgac aaguacggcu ccccggugcg gaucaacauc     1500

gagcucgccc gcgaccuggc caagaacuuc aaggaccgga agcugaucga gaaggagcag     1560

aaggagaacc gcgccaaccu ggacaagauc cgggagaacc uccgcgagcu gauggacaag     1620

gagcccaccg cggucgaggu gcugaaguac cggcucuggg agcagcagcg cggggagugc     1680

gccuacaccc agagccagau cgcccuggag cgccuguucu cccccggcua cugcgagauc     1740

gaccacauca uccccuucag ccgguccuuc gacgacagcc ucuccaacaa gauccuggug     1800

cugggcagcg agaaccagcg caaggggaac aagaucccgu acgaguacuu cggcgaggac     1860

uccgagcggu ggaacagcuu cgagaucugg gucaaggggu ccuaccucaa cuacaagaag     1920

aaggccaacc ugcugaagaa gaaguucacc gaggaggagc agcgcgacug gaaggcccgg     1980

aaccuccagg acacgaagua caucugccgc uacaucagca acuucgugaa caaccggcug     2040

gaguucaagg agagcgagaa caagcagaag gugaucaccg ucaacggccg cgcgaccgcc     2100

uaccugcggu cccgcugggg gcucaacaag gugcgggagg acggcgacaa gcaccacgcc     2160

cuggacgccg cgguggucgg cgugaccacg caggggaugg ugcagaagau cagcaaguac     2220

uccaaggccc acgagcugua caccguccgc aagaacgacg aguucgugga cguggagacc     2280

ggcgagugcg ucgacgugga cgaguacaag gagauccgga aggacauccu cccccgcccc     2340

uggcgggggu ucagcgagga gcugaagcug cgccucuccg acaacccccu ggaggagcug     2400

aagaagcucc cgaucgagac cuacaacgag gaguucauca aggagaacgu gaagcccauc     2460

uucgucagcc gggugcccuu ccgcaagauc uccggcaagc uguucaagga gacgaucuac     2520

agcgagcggg ccuucaagga cggguacuuc guguccaaga agucccuggu cgaccucaag     2580

cgcggcgacc uggacaacuu cuacaacuac gagugcgaca agaagcugua cgacgccauc     2640

agcgagcgga ugaaggaguu caacuacaac ggcaagaagg ccuucgagag ccccuuccgc     2700

aagccgacca aguccgggaa ggagggcccc guggugcgca gcgucaagau caaguccgcg     2760

gcccccuuca aggacgggau ccccaugaac gagggccucg uggccaagga gggcauggug     2820

cggaucgaca ucuacgagaa ggaggggaag uacuucgucg ugccggugua ccgcuaccag     2880

cuggccaagg gcaucguccc caagcgggcc gcgaucgccg gcaagagcga gcugaagugg     2940

cccaucaugg accagaccua caacuucaag uucuccaucu ucaagaacga ccucaucgag     3000

aucaaguacg ccaagaagga cggguacuuc ggguacuacg acggcuucga ccgccacacc     3060

gccagccugg ugaucgagaa ccacgacaac agcgaccggu accgcggcau cggggugaag     3120

acgggcgucg uggaguucaa caaguacgag guggacgucc uggggaacua ccacaaggug     3180

aaccucggcg ggaacuga                                                   3198


<210>  1512
<211>  3201
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1512
augagcgagc gcgugguccg gcgcauccug ggcaucgacc ucgggaucgc cuccugcggc       60

uggggcgugg uggacaucag cggggccggg ggcggcauca ucgcgaccgg gguccggugc      120

uucgacgcgc cccugaucga caagaccggc gagccgaagu ccgccacgcg ccggaccgcc      180

cgcgggcagc ggcgcaucgu gcggcgccgg cgccagcgga ugaacggcgu gcgccggcug      240

cucugcgagu ucgggcugcu gcccgacccc cgccccgacg cccucaacca ggccaugcgg      300

cgcaucagca ccgccuccgc cgccgcgcag gucaccccgu ggacgcugcg ggccgccgcc      360

caccagcgcc ugcucagcaa cgaggagcug gccguggugc ugggccacau cgcgcggcac      420

cgcggcuucc gcuccaacgc caagaacgag gccggggcca acgccgcgga cgagaccagc      480

aagaugaaga aggccaugga ggccacccgg gagggccucg ccaaguacca cgccuucggg      540

gacaugaucg cgaacgaccc caaguucgcc aaccgcaagc ggaaccgcga caaggacuac      600

ucccacaccg ccaagcggag cgaccuggag gacgaggucc gcgccauccu gcgggcccag      660

cuccgcuucg gcuccgcggc cgccacggag acccuggccc agaccuucgc ggacguggcc      720

uucuuccagc ggccccugca ggacagcgag gaccgcgugg gcgacugccc cuucgagccg      780

gggcagaagc gggccgcccg ccgggccccc uccuucgagc uguuccgcuu ccucagccgg      840

cuggcgaacc ucaagcuggc cgucggccgc ucccccgagc ggcgccugac cgccgaggag      900

aucgcccucg ccgcgaaggg guucggcgag acgaagaaga ccaucaccuu caagagccug      960

cgggaggccc uggaccucga ccccaacgcc cgcuucuccg ggaucggcaa ggacaaggag     1020

gccagccugg acgugguggc ccgcaccggc ggggcggccu acggcacgaa gacccugaag     1080

gacgcccucg gggacgcccc guggcggucc cugagccgca cccccgagac ccuggaccgg     1140

aucgcggaga uccucagcuu ccgcgaggac aucacgucca uccggaacgg ccuggaggac     1200

cuggggcucg acagccuggu cguggaggcc cugaugcagg ccgccgccaa cggcgacuuc     1260

aaggaguuca cccgcgcggg ccacaucucc gcccgggccg cccgcaacau cauccccggg     1320

cuccgggagg gccuggugua cagcgaggcc ugcgcgcgcg ucggguacga ccacgccgcc     1380

cggcuguccg ugccccucga ccaggugggc agcccgguca cccgcaaggc ccuguccgag     1440

gcccugaagc agguccgggc gguggcccgc gaguacgggc ccaucgacua cuuccacauc     1500

gagcucgccc ggagcguggg caaguccgcc gaggagcgca agcagcugac cgacggcauc     1560

gaggcgcgga acguggagaa ggccaagcgc cggaagcagg ccgaggagca ccuggggcgc     1620

gcccccagcg acgacgagcu ccugcgguac gagcuggcca aggagcagaa cuucaagugc     1680

aucuacuccg gcgacgcgau cgaccccgcc ggggucgccg ccaacgacac gcgcuaccag     1740

guggaccaca uccugccgug gagccgcuuc ggcgacgacu ccuaccucaa caagacccug     1800

ugcaccgccc ggagcaacca gaacaagcgc ggccggaccc ccuucgagug guucgaggcg     1860

gacaagacgg acguggagug gauggaguac gucgcccgcg uggagaaccu gcaggaggug     1920

aaggggcgga agaagcgcaa cuacuccauc aaggacgccg ccgccaucga ggacaaguuc     1980

aaggcgcgga accucaccga cacccagugg gccacccgcc ugcuggccga cgagcucagc     2040

cggauguucc cgccccgcga gugcgagcgg gccaucugcg gccgcgccga cgggggcaac     2100

gacgggcuga cgaucgucga ggagcggcgc guguucaccc ggcccggcgc gaucaccucc     2160

aagcugcgcc gggccugggg ccucgagggg cugaagaagc aggacggcaa gcgcguggag     2220

gacgaccggc accacgccgu cgacgcccug gugcucgcgg ccaccacgga gagccugcug     2280

cagcgccuca ccguggaggu ccagcgccgg gagcgcgagg ggcggcccga cgacaucuuc     2340

cacugcgccg agcccuggcg cggcuuccgg gccgacgugc gccggaccgu guacgggucc     2400

gagaccaugc ccggcaucuu cgucagccgc gccgagcggc gccgggcgcg cggcaaggcc     2460

cacgacgcca ccaucaagca gauccgggag aucgaggggg agcgccuggu guucgagcgg     2520

aagccggugg agaagcugac ggacaaggac cucgagaaga uccccauccc caagccguac     2580

ggccagguca gcgaccccaa gcgccugcgg gacgagcugg uggagucccu ccgcgccugg     2640

aucgccgcgg ggaagcccaa ggaccggccc ccgcugagcc ccaagggcga cgugauccgc     2700

aagguccgga uccagaccaa cgacaaggug uccguggaga ucaacggggg caccgucgac     2760

cgcggcgaca uggcccgcgu ggacguguuc cggaagaaga acaagaaggc cgucugggag     2820

uacuacgugg ugcccaucua cccccaccag aucgucgccc ugaacgaccc gcccgaccgc     2880

gccgugaucg cguacgccga ggacaaggac uggaaggaga ucgacagcuc cuacgaguuc     2940

cucuggagcc uguucgggcu guccuacgug gagaucagca aggccaacgg cgagugcauc     3000

gacggguacu uccggggccu ccaccgcggg accggcgccg cguccgucug caagcacauc     3060

agccugggca aggacgccac gguguccggg aucggccuga agacccucgc cagcuucaag     3120

aaguucacca ucgaccggcu ggggcgcaag uucgagaucc cccgggaggu gcgcaccugg     3180

cggggcgagg ccugcacgug a                                               3201


<210>  1513
<211>  3201
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1513
augagccacc uggugcucgg ccuggaccug gggcccaacu ccaucggcug ggcccucguc       60

aacgacgacc cggagcaccc cguggagagc aagcuggugg accugggggu ccgcguguuc      120

cccgagggcg uggacaacuu cgacaccucc aaggagcuca gccggaacga ggaccgccgg      180

gucgcccgcg ggaugcggcg ccagauccgg cgccgggcgc gccggcgcca gcagcugaag      240

caggcccuga ucgacuccaa gcucuggccc agcgacccga acgacgaggu ggugcuguac      300

aacaccaacc ccuacgagcu gcgggcccgc gcccucaagg agcggcugga gccccacgag      360

gucggccgcg ugcugcucca ccugaaccag cggcgcggcu uccuguccaa ccggaagaag      420

gaccgcgggg acaaggaggu gcagggcaug cucgccgaga ucaacgagaa cgagcagcag      480

ugcaaggacg ggggcuucga cacgaucggg gcguggcugg ccgacaagca ggccaacauc      540

gaccacgcca accgggagga gaacgaccac guccgcaacc gccaccuggc gcggagccag      600

uacguggacg aguucgaggc caucuggucc gugcaggcca agcaccaccc caagcuccug      660

accgaggagc ugaaggucgg caagcucggc aagcagaccu acccggugaa gcccaucccc      720

cgcuggcacg agcaccggca ggggcugagc ccccuggagu ccuucggcau cuacgggcuc      780

aucuucuucc agcgcccgau guacuggccc aagagcgugg ucggccugug cgagcuggag      840

cccaaggagc ggcgcugcca gcgguccgac cgccggcagc agcgcuuccg gcuccugcag      900

gaggugaaca accugaagua cguggacccc gacacccacc ucgagcagaa gcuggacgac      960

aagcagcgcc ggcugcuccu ggacaagcug agccacacga aggagaugac cuucgaccag     1020

auccgcaagg cccucgacuu ccuggagucc gucaaguuca accuggagcg gggcgagcgc     1080

agccggcuca agggggugcc gaucgacgcc cuguucgcgg ccaaggacgu gcugggccag     1140

gagugguacg accgccccga gcagcagaag accgagaucg ucgccgugcu ccuggacaac     1200

gagcgggacg acgaccgcau caucgcccgc gccgucgcgg agugggggau gaccgccaag     1260

gaggccgagg ccaugcuggg cguggaccuc cccgccgggu acgcgaaccu guccugcaag     1320

gccaucgaga agcugcugcc ccacauggag cggggccucc uguacaugga cgacgacgag     1380

agcaacuccg ccaugcacgc cgccggcuac cugcgccggg accagcuccg ccggcgcauc     1440

uucgacgugc ugccggaccc cgcgcggacg cccaacugcc ccaucgggga caucccgaac     1500

cccgucguga agcgcacccu gaccgagauc cggaaggugg ucaacgccau cgugcgcgag     1560

uacggcaagc ccgacgccau ccaccucgag cuggcccgga gcgugcagau ggggaaggag     1620

aagcgcagcg aguacuccaa gaugauccgg gaccgcgagg cggagcggga cggcaucguc     1680

gagaagcugc gcgagagcgg ggugcgggug acccgcgaca acauccucaa guaccagcug     1740

ugggagcagc agaaccagga gugcaucuac uccggcaagc ccaucagcuu cgagaagcug     1800

uucggcgagg gcggcggggu cgagguggac cacauccucc cgcggucccg cagccuggac     1860

gacucccagg ccaacaagau cgugugccug cggacggcca acgccgggaa gggggaccag     1920

acccccuacg aguggcucgc cgacacccag cccaagcagu acgagcaggu cugccagcgc     1980

gcgggcaugc ugaugcgcag cggccggaug ccguacgcca aguaccgccg guucauccag     2040

cgcgagcugg agcucgacaa guucaucgcc cggcagcugg ccgacaccgg guacaucacg     2100

aaggccacca uggaguaccu gcgcuaccuc uucgacaagg accacgacgu gcugggccug     2160

aaggggcagc ucaccgcgga gcugcgguac cacuggggcc uggagaccau ccucgaggag     2220

cugcccgacu cccccgccug gcacgaggcc gacagcggca agcugcgccc cggggagaag     2280

aaccgggccg accaccgcca ccacgccauc gacgcggugg ucaucgcccu cacgaaccgg     2340

cgccggcugg ugcagcuguc cgacaucgug aagcgcggcg gggcccgggc ccacggcgag     2400

auccucgagg acccguggcc caacuuccgc gagaacgucg ugcaggcgau caagaagacc     2460

aacgugagcc accgggucga gcgcaaggug gccgggaagc ugcacgagga gacccuguac     2520

ggccccaccc ggacgcccgg cgagugggug cuccgcaagc cggucaucgc ccuguccgcc     2580

aacgaggugg agcgcauccg ggacgccacc auccgccaga ucguggucag cgcgcuggcc     2640

gacgccggga ucgacguggg ccgggggaag aagcccgacg ccaagaagau gaaggaggcc     2700

cucuccaagc ugaagaugcc cagcggcgug cccauccgca agguccggcu gaccaagccg     2760

gagcagacca uccagcccgu gcgcgcgggg cggcccgacc aggccuucgu gaagcccggc     2820

uccacgcacc accucugcau cuucgaguuc accgagaacg gcaagaccaa gcgcgacgcc     2880

gucuucguga ccaugcugga ggccaugaac cggcugaagc gccgggagcc ggugauccag     2940

cgcacgcacc ccgagcggcc cgacagccgc uucgucaugu cccucagcgc ccgggagaug     3000

cugcuggcga acuggaagga cggggagcgc cuccugaccu ucaagaccgc cagcuccacc     3060

cagggccaga ucugguucgc cgagcacacg gacgcccgca agagcgcgga cuacaagcag     3120

uucguggcca acgccaacac ccuggacgcc aagaagguga ccgucgaccc ccucgggcgg     3180

auccgguggg ccaacgacug a                                               3201


<210>  1514
<211>  3204
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1514
augggcaccc gccuggggcu cgacaucggc accaacagca ucggguggug ccuguucaac       60

cuggacggcu ccgggcggcc caucggcauc cgcgacaucg gcgugcggau cuucagcgac      120

gggcgcgacc cgcgguccgg cgccagccuc gccgucgagc gccggguggc gcgcgccaug      180

cggcgccggc gcgaccggua cauccgccgg cgcuccgccc ugcuggaggc ccucaucaac      240

acggggcuga ugcccgccga cgugcaggag agcaaggcgc ugaccggcug cgaccccuac      300

guccuccggg cccaggcccu ggaccgcgcc cuggacgcgc accuccuggg gcgggugcug      360

uuccaccuca accagcgccg gggcuucaag uccaaccgca aggccgagcg caaggccggc      420

gacgacaagg agggcgggaa gaucgccgac ggggccaagg cgcuggaccu ggccaugcac      480

gaggccggcg cccggaccua cggcgaguuc cucgccgggc gcgacagccg gcgcgugcgg      540

cugcgcgcgg agggcgacgg guacgacuuc uaccccgagc ggcgccaccu ggaggccgag      600

uucgacgcca ucugggacgc ccaggccgcg uacaacccgg cccuccugac cgacgucgug      660

cgggaccgcc ugcggcgcau caucuucuuc cagcggcccc ucaaggcccc caaggugggc      720

gggugcacgu ucuucaacga ggagccccgc cugccgaagg cccacccccu guuccaggag      780

cggcgccucu acgaggaggu caaccagcug gagaucaccc gggccggcgc gcccucccgc      840

aagcugaccc ucgaccagcg ggacaagauc auccgcaagc ucaagagcgc caagaccgcc      900

uccuucagcg gccuggcccg ccugcugaag cuggcggacg gggaguccuu cagccgggug      960

acggagaacc gcaccggccu cgccggggac gacaucuuca ccuccuucag cgacaagcgg     1020

ugcuucggcg accgcugggc ccacuucgac cccggggugc agugguccau caucgagacc     1080

gucacggagg aggaggaccc ggugcggcug cgcgacuggc ugauggccac ccacggcgug     1140

accgucgagc aggccgaggc gaucggccgg gugcgccucc ccgaggggca cggccggcug     1200

ggggagaccg ccagccgccu gauccucgcc cagcugaagu ccggcagcaa ggacgggcac     1260

ccccugacgu acaacgaggc cguggaggcc gcgcucggca agagccacuc cgacuuccgg     1320

accggcgaga uccuggagca gcugcccuac uacggggaga uccucagccg cgagaucccg     1380

cccggcuccc uggaccccga cgacgacgac gagauccggu gggggaagau caccaacccc     1440

accguccaca ucggccugaa ccagcugcgc cggcuccuga acgcccugau caaggcccac     1500

ggccgcccgg acgagaucgu gguggagcuc gcccgggagc ugaagcugag cgagaaggac     1560

aaggccgagc acaaccgccg gaucggggcg aacacgcgcg ccgccgaggc ccgguccaag     1620

aagcuccugg agagcggcca gcccgacacc ggggcgaacc gcucccugcu caagcugugg     1680

gaggaccuga accccaacaa cccccucgac cgcugcugcc cguucugcgg ccggcacauc     1740

gggcagaugg cccuguucag cggcgagacc gagaucgagc acaucauccc cuacucccgc     1800

agccuggacg acuccgccgc caacaagguc aucgcccacc ggagcugcaa ccgcgagaag     1860

ggcaaccgga cccccuggga ggcguggggg cacacggagc gcugggccau caucgccgag     1920

caggucuccc ggcuccacaa gagcaagcag uggcgcuucg cccccgacgc caucgagcgg     1980

uucaaccagg agggcggguu caucgcgcgc cagcuguccg acacccagua ccugagccgg     2040

auggcccgca ccuaccucuc cagccuguac ccggacaccg ccggcgugua cgugaucccc     2100

gggcggauga cggccaugcu gcgccggcuc uggggccuga acgagcugcu ccccgaccac     2160

aacuacgugg agaacccgca cuccggcgcc cccaagaacc gccuggacca ccggcaccac     2220

gcgaucgacg ccgccgucgu gggggugacc acccgcggcc ugcucaacga gaucagccgg     2280

gccgcggggc gcgccgagcg ccaggaccug gaccggcugu ucaucgaccu ccccaagccc     2340

ugggagaccu uccgcgagga ccugcacgag agccuggccc gggucguggg cucccacaag     2400

gccgaccacg ggcgcaaggg cgugccggcc aagggcaagg acgcgacggc cgcccggcuc     2460

cacaacgaga ccgccuacgg gcugaccggc cugaccagcg aguccgggcu ccccaucguc     2520

gugcaccgcg ugccccugcu gagccucaag cccgacgacc uguccaaccc gauccggauc     2580

cccgacgccg cgcugcagcg cgcccuccgg gaggccacgg acggcaagag cgggaaggac     2640

uuccaggagg cccuggccca guucuccaag agcgaccccg ucuucaaggg cauccgccac     2700

gugcgggugc gcgagacccu gaaggucauc cccauccggg acgcguccgg ccacgccuuc     2760

aaggccuaca agggggacgc caacgcccgc uacgacgugu ggcggaugcc ggacggcaag     2820

ugggugagcu ccuggcgcga ccgggacggg aaggcgcaca gcggcaucau caccauguuc     2880

gccgcccacc aggccggcca gcccguccag cgcccccacc ccgcggccaa gcgggugcuc     2940

ucccugcgcc agaacgaccu gguggccguc gagccgcagg ggcagccccg gaagaucaug     3000

cgcgugguga aguucagcuc cggcgggcag aucaccuucg ccgagcacca ggaggccggc     3060

ccccucaagg cgcgcgacgc caccagcccc gacaucgacc cguucaagua cuucuacucc     3120

agcgccgggg gccugcagaa ggcccgggcc cgccagaucc ggaucgacga gcugggccgc     3180

aucuucgacc ccgggccccg guga                                            3204


<210>  1515
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1515
augaaguacg ugcugggccu cgacaucggg aucgccagcu gcggcugggc cgucaucaac       60

caggagaagc accgcaucga ggaccugggg gugcggaucu ucgacaaggc ggagaacccc      120

aaggacggca agucccuggc caccccgcgc cgggacgccc gcagcacccg gcgcacgcuc      180

cggcgcaaga agcaccggau gcagcgcauc aagauccugc uggugaagca cgggcuccug      240

uccaagaccg agaucgacca ccuguacgag agcgccaccg agaucgacgu cugguaccuc      300

cggcugaacg cccuggagcg ccggcucaac cccaaggagu ucgcgcgcgu gcugauccac      360

cuguccaagc ggcgcggcuu caagagcaac cggaaggaga ccacgcucuc cgagaacggc      420

cagauccugg agaacaucag cgagaaccug cagaucaugg agcagaagaa cuaccgcacc      480

gugggggaga ugauccucaa ggacaagcgg uucgagaacc acaagcgcaa caaggacggc      540

accuacaucg ggaccgucac gcggcagcag cugcaggagg agauccagau gaucuucaac      600

gcccagcgcc uguacaagaa caaccacgcc accgagaagu ucgaguccag cuaccucgag      660

aucugggccu cccagcgccc cuacgcgagc aaggaccaga ucgagaagau gaucggcuac      720

ugcacccugg agcccaagga gaagcgggug ccgaaggccu ccuggagcuu ccaguacuuc      780

guggcccugc agaccaucaa caaccuccgc cugaucucca aggaccggau cgaggagcug      840

agcuucgagg agaagaagcg caucaugaac cucgcccugg agaaguccau cgucaaguac      900

aucgacaucc ggaagcugcu cagcgugccc aaggaguucc aguucaacuc ccugagcuac      960

agcgccgaca ucguggacac gaccgucgag aacaagaagu gcaucgagcu gaaggaguac     1020

cacuccauca acaaggugua caagcagauc uacgggcaga acgcgcucaa ccugcugccc     1080

aaggacuacg acaccaucgc cuacggccuc accaucuuca aggacgacaa ggacauccag     1140

gaguaccugc agaacaagua cgugaacgcc aagggcaagc ccaucagcaa ccuggccaac     1200

aagacguacg acgacaccuu cauccagguc cuccugaccc ugaacuucuc caagaugggg     1260

caccucagcu ucaaggcccu gaagaacauc aucccguucc uggaggaggg ccucuccuac     1320

gacaaggcgu gcgagaaggc cggguacaac uucaagggca ccagcuacac gaagaagacc     1380

aaguaccugc ccgugauccc ccagaacacc aaccccgugg uccaccgcgc ccugucccag     1440

acccggaagg ugaucaacgc caucaucaag aaguacggca gcccgaacgc cauccacauc     1500

gagacggcgc gcgagcucuc caagaccuuc caggagcgga aggagaucga cagcauguac     1560

caggacaacu ccaagaagaa cgagcacgcc aucugcaagc ugaaggagcu ggggaacauc     1620

aaccccagcg gcaucaacau cgugaaguuc aagcugugga acgagcagga cgggcgcugc     1680

auguacaccg gcaaguacau cgagccccac cgccucuucg aggaggggua caccgagguc     1740

gaccacaucc ugcccuacuc ccggagccug gacgacuccu acaacaacaa ggugcucacg     1800

cugggcaccg agaaccagcg gaagggcaac aagaccccgu acgaguacau gcgcaacaac     1860

agcaucuggg acgaguucga gacccgggug caguccaaca agaagaucaa caagaagaag     1920

cagcagaagc ugcuccugca gaacuucagc cacgcccgcg agcaggaguu caucgagcgg     1980

aaccugaacg acacgcgcua cgccaccauc uaccucucca gccugaucca gcagcaccug     2040

aucuucagcg aguccagccg ggagaagaag guccacaccg uguccgggau caucaccagc     2100

caccuccgcu cccgcugggg cuucaacaag gaccggaagg aggggcacau ccaccacgcc     2160

cuggacgcgg ucaucguggc cgugacgagc gaccacauga uccagcgcgu caccaaguac     2220

uacaagcuga aggagcucaa ccggaaccgc caggccaagc ggaugcaguu ccccgagccc     2280

ugggagggcu uccgccugga gcuggaggcc cggaucuccc ccaacaccca gcaguaccuc     2340

aaggggaucc gcuucaagaa cuacgcggac gugaaccuga gcgagaucaa gccgaucuuc     2400

gugucccgga ugcccaagcg cagcaucacc ggcgagcugc accaggagac cauccggaag     2460

uuccucggcu acaacgagaa ggggaagguc cugacggcca ccaagaccaa gcuggaggac     2520

auccccuucg acgccaacgg cgacuucccc auguucggga aggagaccga ccucuacacg     2580

uacaacgcca ucaaggagcg cuaccugucc cacaagaagg acaagagcaa guccuuccag     2640

gagccgcugu acaagcccac caagagcggc gggaucggcc cccucaucaa guccaucaag     2700

aucauggaca cccggaccau cgugaacccc gugaaccagg gcaagggggu cguguacaac     2760

agcaagaucg cccgcacgga cguguucaag aagggcgaga aguacuaccu gaucccgauc     2820

uacaccaucg accugaugaa gaacacccuc ccccagaagg cgaucaccgc cgggaagggc     2880

uacgaggacu ggacgaccau cgaccccucc uucaccuucc uguucagccu guucccgaac     2940

gaccucaucc agaucguccc cagcaagaac aagaccauca aggcccggac gaccgugucc     3000

aagaaggagg ugcugcugcc cagccucacc ggcuacuuca agggggucca cuccggcacc     3060

gccgggauca cgguggagac ccacgacggc agcaucaucg ccaacguggg guccaagcag     3120

cugcugagcu ucgagaagua ccaggucgac gugcucggcc acuacaccaa gaucaagaag     3180

gagaagcgca ucggcauggc gaucuga                                         3207


<210>  1516
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1516
augaacaagg ccguggccua ccgccuggcg cucgaccugg gcagcaccuc ccuggggugg       60

gccaucuucc ggcucaacga ggcccgcgag cccaccgcca ucauccgggc cggcguccgc      120

aucuucagcg acgggcggaa cgcgaacucc gagccgcugg ccgugcaccg ccggguggcc      180

cgcgccaugc ggcgccggcg cgaccggcug cucaagcgca agaagcggau gcacgaccag      240

cugguccagc acggcuucuu ccccgcggag guggccgagc gcaaggagcu ggagcggcuc      300

aaccccuacc agcugcgcgc caaggggcug cacgaggccc ucacgcccgg cgaguucgcc      360

cgggcgcugu uccacaucaa ccagcgccgg ggcuucaaga gcaaccgcaa gaccgaccgc      420

aaggacaacg acuccggggc ccugaagcag gccaucagcg agcuccggaa gcgcauccag      480

gacuccgacu gcgccaccgc cggcgagugg uucuggaagg agcggaugca gcagaagccg      540

gagggggugc gcggccagug gguccgggcg cgcuaccgga agacccccag cacgaccgac      600

gaggggaaga agcgcaucgg cuacgaccug uacguggacc gggccauggu ggagcaggag      660

uucgacgccc ugugggccgc ccaggcggcc cuccagcccg gccuguucac cgaggccgcc      720

cgcgccgagc ugaaggacac ccuccuguac cagcgggacc ugcgccccgu caagccgggg      780

cggugcacgc uccugcccgc ggaggagcgc gcgccccugg cccuccccuc cacccagcgg      840

uuccgcaucc ugcaggaggu gaacaaccug cggcuccugg acgaggcccu gcgcgaggug      900

ccgcucaacc uggcccagcg ggacgccguc gugagcgccc uggagggcaa gaaggagcuc      960

uccuucgccg ccauccgcaa gcugcugacc cucagcggga aguucaaccu ggaggacgag     1020

aagcgcgcgg agcugaaggg caacgccacc uccgugaucc ucgcccggaa ggaccuguuc     1080

ggcgacgccu gggccggguu cgacgcggcc guccaggacg agaucgugug gcgccuggug     1140

agccaggagu ccgagggcgc ccucaucgcc uggcugcagc agcacacggg ggucgacggc     1200

gugugcgccg aggcgaucgu gaacacccgg cugcccgacg gguacggccg ccucagccgg     1260

aaggcccugg agcgcaucgu ccccgcccug cagcgggagg ugugcaccua cgacaaggcc     1320

gugcaggcgg ccggcuucgc ccaccacucc gaccuggggu ucgacuucga cuacgccgag     1380

gacgaggucc agcaggucgg cgagcacacc aucgccagca cgggggaggu gcaggcgcag     1440

uacgccuuca agcagcuccc cuacuacggc aaggcccugc agcgccacgu ggccuucggg     1500

uccggcgacg ccaaggacca cgaggagaag ugcuacggca agaucgcgaa cccgaccgug     1560

cacaucgggc ugaaccaggu ccggaccgug gugaacgccc ucauccgccg guacggccac     1620

cccaccgagg ucguggugga gcuggcccgc gaccugaagc agagccggga gcagaagcag     1680

cagacgcagc gcgagcaggc cgacaaccag aagcggaacg aggacauccg caagcggauc     1740

gcccccaucc ucgagaccag ccccgagcgc guccgggacc gcgacaucaa gaaguggauc     1800

cugugggagg agcugaacaa gaaggacauc gcggaccggc acugcccgua cuccggggag     1860

cgcaucagcg ccaccaugcu ccuguccgag gccguggaga ucgagcacau ccugcccuuc     1920

agccgcaccc ucgacgacuc ccugaacaac cggacggugg ccaugcgccg ggcgaaccgc     1980

aucaagggcg accggacccc cugggaggcc cgcgccgacu ucgaggccca gggguggcgg     2040

uacgaggcca uccugcagcg cgcggagggc augcccccgc ggaagcgcua ccgguucgcc     2100

gaggacggcu accagcgcug gcucgggaag gaccggaacu uccuggcccg cgcccugaac     2160

gacaccagcu accucucccg gcuggccgcg aacuaccugc gccucgucug cccccagggc     2220

gugcggguga uccccgggca gaugaccgac aagcugcgcg gcaaguucgg ccugaacagc     2280

guccucgggc uggacggcaa gaagaaccgg aacgaccacc gccaccacgc cguggacgcc     2340

ugcgugaucg gggucacgga ccagggccug augcagcgcu ucgccaacgc cuccaagcag     2400

gcgcgggaga acgggcucac ccgccuggug caggacaugc ugcuccccug guggccgagc     2460

uacuacgacc acguggagcg ggccguccgc cacauccggg ugucccaccg ccccgaccac     2520

ggcuucgagg gcgccaugau gaaggggacc gcccacggca uccgggagga cgggauccgc     2580

gaggacggcc ggaucaagca gcgccccaag gccaagggga gcgcggacca caagaccauc     2640

acgcugaucc cgaucgacga gccccggcag cuggcccgcc acggcgugga cgccgagggc     2700

aagccccucc ccuacaaggg guacgccucc ggcagcaacu acugcaucga gaucaccaag     2760

aacgggaagg gcaaguggga ggggcagguc aucuccaccu ucgacgcgua ccggaucaag     2820

gccgccgccg acgccgcggc ccgcgccggc caggugauca gcaccgugga ggccgacucc     2880

aucguccgca agagcggcug ggagcggcug cgcggggccc agucccagaa cggccagccg     2940

cuggugaugc ggcucgugau cggggacagc guccggaugg agguggacgg ccgcgacgag     3000

gugaugcggg ucgugaagau gagcgggaag gagauggugu ucgcgcccgu ccgcgaggcc     3060

aacguggaca agcgcaacaa caugcccgac gagcaggacc ccuucacgua caccuacaag     3120

cgggccgacc agcugcgcaa ggccaaggcc cgccagguga ccaucucccc gaucggcgag     3180

cugcgggacc ccggcuucaa gggguga                                         3207


<210>  1517
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1517
augcaguacg ugcugggccu cgacaucggg aucgccagcu gcggcugggc cgucaucaac       60

caggagaagg agcgcaucga ggaccugggg gugcggaucu ucgacaaggc ggagaacccc      120

aaggacggca agucccuggc cgccccgcgc cgggacgccc gcagcacccg gcgcacccuc      180

cggcgcaaga agcaccggau gcugcgcauc aagauccugc ucgugaagca ggggcugcug      240

uccaagaacg agcucaacca ccuguacgag agcacgaccg aggucgacgu guggaaccug      300

cggcucgacg cccuggagcg caagcugaac cccaaggagu ucacccgggu gcucauccac      360

cuggcgaagc gccggggcuu caaguccaac cgcaaggaga ccacgcugag cgagaacggc      420

cagauccucg aguccaucag cgagaaccag cagaucaugg agcagcagaa cuaccggacc      480

gucggggaga ugauccugaa ggacaagaag uucgagaacc acaagcgcaa caaggacggc      540

accuacaucg ggaccgugac gcggcagcag cugcaggagg agauccagau gaucuucacc      600

gcccagcgcc ucuacaagaa cgacuacgcc accgaggagu ucgagaucuc cuaccuggag      660

aucugggcca gccagcggcc cuacgcgucc aaggaccaga ucgagaagau gaucggcuac      720

ugcacccugg agcccaagga gaagcgcgug ccgaaggcca gcugguccuu ccaguacuuc      780

gucgcccucc agacgaucaa caaccugcgc cugaucagca aggaccggau cgaggagcuc      840

uccuucaagg agaagaagca gaucaugaac cuggcccugg agaaguccau cgugaaguac      900

aucgacaucc gcaagcuccu gagcaucccc aacgaguucc aguucaacag ccuguccuac      960

agcgccgaca ccguggacac cacccucgag aacaagcggu gcaucgagcu gaaggaguac     1020

uacagcauca acaaggucua caagcagauc uacgagaaga acgcgcugaa ccuccugccc     1080

aucgacuacg acacgaucgc cuacgggcug accaucuuca aggacgacaa ggacauccuc     1140

gaguaccugc agaacaagua cgugaacgcc aagggcaagc ccaucaacaa ccuggccaag     1200

aagaccuacg acuacaccuu cauccaggcc cuccugacgc ugaacuucac caagaugggc     1260

caccucuccu ucaaggcgcu gaagaacauc aucccguucc uggaggaggg gcucagcuac     1320

gacaaggccu gcgagaaggc cggcuacaac uucaagggga ccuccuacac cgagaagacg     1380

aaguaccugc ccgugauccc ccagaacacc aaccccgucg ugcaccgcgc ccugagccag     1440

acccggaagg ugaucaacgc caucaucaag aaguacggcu ccccgaacgc gauccacauc     1500

gagaccgccc gcgagcugag caagacguuc caggagcgga aggagaucga cuccauguac     1560

caggacaaca gcaagaagaa cgagcacgcc auccacaagc ucaaggagcu gggcaacauc     1620

aaccccuccg ggaucaacau cgucaaguuc aagcugugga acgagcagga cggccgcugc     1680

auguacagcg ggaaguacau cgagccccac cgccucuucg aggagggcua caccgaggug     1740

gaccacaucc ugcccuacuc ccggagccug gacgacuccu acaacaacaa gacccucacc     1800

cuggggacgg agaaccagcg gaagggcaac aagaccccgu acgaguacau gggcaacacc     1860

agcaucugga acgacuucga gacccgcgug cacuccaaca agaagaucaa caagaagaag     1920

cagcagaagc ugcuccugca gcacuucagc uacgcccggg agcaggaguu caucgagcgc     1980

aaccugaacg acacgcggua cgccaccauc uaccucucca gccugaucca gcagcaccug     2040

aucuucuccg agagcagccg caagaagaag guccacaccg uguccgggau caucaccagc     2100

caccuccggu cccgcugggg cuucaacaag gaccggaagg aggggcacau ccaccacgcg     2160

cuggacgccg ugaucgucgc cgugaccagc gaccacauga uccagcgcgu cacgaaguac     2220

uacaagcuga aggagcucaa ccgcaaccug caggccaagc ggaugcccuu ccccgagccc     2280

ugggagggcu uccgccugga gcucgaggcg cggaucuccc cgaacaccca gcaguaccug     2340

aagggguucc gcuucaagaa cuacgccgac gugaaccuga gcgagaucaa gcccaucuuc     2400

gugucccgga ugcccaagcg cagcaucacc ggcgagcucc accaggagac cauccggaag     2460

uucaucggcu acaacgagaa ggggaagguc cugacggcca ucaagaccaa gcuggaggac     2520

auccccuucg acgccaacgg cgacuucccg auguacggga aggagaccga ccucuacacc     2580

uacaacgcca ucaaggagcg cuuccugucc cacaagaagg acaagcgcaa gagcuuccag     2640

gagccccugu acaagcccac gaaguccggc gggaucggcc cccucaucaa gagcaucaag     2700

aucaucgaca cccggaccau cgugaacccg gugaaccagg gcaagggggu cguguacaac     2760

uccaagaucg cgcggaccga cguguucaag aaggacgaga aguacuaccu gauccccauc     2820

uacacgaucg accugaugaa gaacauccuc ccccagaagg ccaucaccgc cggcaagggg     2880

uacgaggacu ggaccaccau cgacccgagc uucacguucc uguucucccu guuccccaac     2940

gaccucaucc agaucguccc cagcaagaac aagaccauca aggcccgcac caccgugucc     3000

aagaaggagg ugcugcugcc cagccucacg ggcuacuuca agggcgucca cagcgggacc     3060

gccggcauca ccguggagac ccacgacggg uccgugaucg cgaacgucgg cagcaagcag     3120

cugcugcuca ucgagaagua ccagguggac auccuggggc acuacacgaa gaucaaggag     3180

gagaagcgga ucggcauggc caucuga                                         3207


<210>  1518
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1518
augcccaaga gcgccaagcc gcagcaggcc uacacccugg gccucgacau cgggaucgcg       60

uccgugggcg ccgcccugcu ggggcagcag cacaucaucg gccuccacgu ccgcaccuuc      120

gaccgggccg agacggccaa ggagggggag ccccugaaca agauccgccg ggacgcgcgc      180

cugacccggc gccggcucca gcgccgggcc caccgccugc ugcggcuccg ccggcuguuc      240

aagcgccaca accugauccc cgacaccgac accagcacgu ucaucuccgg ccagagcccc      300

ugggagcucc gggcccaggg ccuggaccgc cagcugaccu ccgccgagug gaccaccgug      360

cucuaccaca ucgugaagca ccggggguuc cacagcaacc gcaaguccga ggucaagacg      420

gacgagaagg cgggccagau gcugagcggg gugaaccaga accaggcccu gcucaagcag      480

uccggccugc ggaccauggg ggagcuggcc gcccgccacg aggccuuccg ggaggcgaag      540

cgcaacaaga agggcgccua caaccacacc uucagccgcc acgaccucga ggacgagcug      600

caccuccugu ucucccggca gcgcgagcug ggcaacccgu acgccacccc cgagcuggag      660

gacgccgugc acacgcuccu gaugcagcgg cgccccgccc ugagcgggac caaccuccug      720

aagauggucg gccacugcac ccuggagccc gacgaguucc gggcgccgaa ggccuccuac      780

accgccgagc gcuucaucug gcucacgaag cugaacaacc ugcgggugag cggguccggc      840

gagacccgcc cccucaacga gagcgagcgg caggcccugc uguccaagcc cuucagccag      900

uccaagcuca ccuacaagca gguccgcaag auccuggagc ugcccgacaa cauccgguuc      960

gugggccucc acuaccaccc ggcccaggac aacggcaaga gccccgagaa ccgcguguuc     1020

uucgaggcga aggccuucca cgcccugcgg aacguguaca agaaggccgg gcugaaccuc     1080

gaguggcagc gcgacagcca gaaccccgag aagcuggaca acaucaccua cgcccugacg     1140

gucuucaagg aggacaagga ggcgaagcag uggcucaucg agaagggggu ggaggccucc     1200

gugaucgagg ccguccugaa cgagagcuuc uccgaguucg ugcggcugag caucaaggcc     1260

cugcgcaaga ucauccccca cauggagauc ggccaccggu acgacgaggc ggugaagcuc     1320

gccggguacu cccaccacag ccaccucccg cagcccucca agacccccua caucccccgc     1380

uucagcaagc aguccaucgc caacccgguc gugagccggg cccugaacca ggcccgcaag     1440

cuggugaacg cgaucguccg cgaguacggc ccgcccgacg ccgugcacau cgagcuggcc     1500

cgggaccuga acaagcccuu cgacgagcgc cggaagaucg agaaggacca ggagaaguac     1560

cagaaggaca aggcccgcga caucgagacc uuccaggaga acuucggcuu cacccccaag     1620

gggcuccagc ugacgaagug gcggcuguac cgcgagcagu ccggcaagug cgccuacagc     1680

cucaagcccc uggacaucaa ccggcuguuc gaggacgggu acguggagau cgaccacgcg     1740

cuccccuacu cccgcagcuu caacgacggc augaacaaca agguccugac ccugaccacc     1800

gagaaccgga acaaggggaa ccagacgccc uacgaguacc ucgacggcgc cuccaacagc     1860

gcccaguggc gcaacuucgu ggccuggguc aacuccaaca agagcuaccg ggccuccaag     1920

cagcgcaacc ugcugcggaa ggacuucgcg gcccaggccg ccaccgaguu ccgcgagcgg     1980

caccucaccg acacccgcua caucgcgcgg gaguucaagc gcauggugga gacgcaccug     2040

aagcugaaca agccgaacca gcggugcgug gucgugagcg gccagcucac cgccuaccug     2100

cgcgcccggu gggggcugau caaggugcgc gagaacggcg accuccacca cgcccuggac     2160

gccgcggucg uggccgccug cagccacgcc auggugaagc gccuggccga cuacucccgg     2220

cgcggggagc uccaguacgc gcggggccac cacuucgacc ccgagaccgg ggagaucauc     2280

gacaucaacg cccugcgcaa gcuggacgac cacuuccccc agccguggac cgacuuccgg     2340

cgcgagcucc uggccuggcu gagccccgac cccgcccagc agcucaagaa ccuggccuac     2400

uacacggacg agcggcugaa gcaggucaag cccauccgcg ugucccgggc gccgacccgc     2460

cggggccucg gcgccgccca ccaggagacc auccgcagcg ccaaguaccu gggggagaac     2520

cgguccaccg ugaagacgcc ccuggagaag cucaagcuga aggacauccc caacaucguc     2580

ggcuacgagg acccccgcaa ccaccagcug auccaggcga ucagccagcg gcucaccgag     2640

cacggggacg acggcaagaa ggccuucaag gagccgcugu acaagcccgg caacaacaag     2700

uccaccgccc ccguggugcg cagcgucaag cuguucaccg ugcagaaguc cgggaucccc     2760

gugcggggcg ggaucgccaa caacggcaac augguccgcg uggacaucuu caccaagaag     2820

gagcgguacu ucgccgugcc ggucuacgug agcgacgcgg cccgcaacga gcuccccaac     2880

aaggccgugg cccgcgggaa ggacggcugg aucgacaucg acgacuccuu cagcuuccug     2940

uucucccugu accccaacga cugggucagc guggugguca agccccacga ggccccgugc     3000

gagggcuacu acgcggggcu cgacaucucc acgggcgcca uccacaucug ggcccacgac     3060

cggaaccgca gcguggggaa gaacggccug augcggucca ucgggaucaa gaccgcccac     3120

agcguggaga aguaccacgu cgaccugcuc ggcaaccugu accgcguggu gcacgagacc     3180

cggaagcccc ugcgcgccgg caaguga                                         3207


<210>  1519
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1519
augcaguacg ugcugggccu cgacaucggg aucgccagcu gcggcugggc cgucaucaac       60

caggagaagg agcgcaucga ggaccugggg gugcggaucu ucgacaaggc ggagaacccc      120

aaggacggca aguccauggc caccccgcgc cgggacgccc gcagcacccg gcgcacgcug      180

cggcgcaaga agcaccggau gcagcgcauc aagaucaucc ucgugaagca ggggcugcug      240

uccaagaccg agcucgacca ccuguacgag agcgccaccg aggucgacgu guggaaccug      300

cggcucgacg cccuggagcg caagcugaac cccaaggagu ucacccgggu gcucauccac      360

cuggcgaagc gccggggcuu caaguccaac cgcaaggaga cgacccugag cgagaacggc      420

cagauccucg aguccaucag cgagaaccag cagaucaugg ggcagaagaa cuaccggacc      480

gucggcgaga ugauccugca ggacaagaag uucgaguccc acaagcgcaa caaggacggg      540

accuacaucg gcacggugac ccggcagcag cugcaggacg agauccagau gaucuucaac      600

gcccagcgcc ucuacaagaa caacuacgcc accgccgagu ucgagaacag cuaccuggag      660

aucugggcgu cccagcggcc cuacgccagc aaggaccaga ucgagaagau gaucggguac      720

ugcacccugg agcagaagga gaagcgcgcc cccaaggccu ccuggagcuu ccaguacuuc      780

guggcccucc agacgaucaa caaccugcgc cugaucucca aggaccggau cgaggagcuc      840

agcuucgagg agaagaagca gaucaugaac cuggcgcugg agaaguccau cgucaaguac      900

aucgacaucc gcaagcuccu gagcgugccg aacgaguucc aguucaacuc ccugagcuac      960

agcgccgaca ccguggacau caccgucgag ucccggaagu gcaucgagcu caaggaguac     1020

cacagcauca acaaggugua caagcagauc uacgagaaga acgcccugaa ccugcucccc     1080

aaggacuacg acaccaucgc cuacggccug acgaucuuca aggacgacaa ggacauccug     1140

gaguaccucc agaacaagua cgugaacgcc aagggcaagc ccaucuccaa cuucgcgaac     1200

aagaccuacg acgacaccuu cauccaggcc cugcugaacc ucaacuucac caagaugggg     1260

caccugagcu ucaaggcccu gaagaacauc auccccuucc ucgaggaggg ccuguccuac     1320

gacaaggccu gcgagaaggc cggguacaac uucaagggca cgagcuacac cgagaagacc     1380

aaguaccugc cggucauccc ccagaacacc aaccccgugg ugcaccgcgc gcucucccag     1440

acgcggaagg ucaucaacgc caucaucaag aaguacggca gccccaacgc cauccacauc     1500

gagaccgccc gcgagcuguc caagaccuuc caggagcgga aggagaucga cagcauguac     1560

caggacaacu ccaagaagaa cgagcacgcc aucugcaagc ugaaggagcu cgggaacauc     1620

aacccgagcg gcaucaacau cgugaaguuc aagcugugga acgagcagga cgggcgcugc     1680

auguacuccg gcaaguacau cgagccccac cggcuguucg aggaggggua caccgaggug     1740

gaccacaucc ugcccuacag ccgcucccuc gacgacagcu acaacaacaa gacgcugacc     1800

cugggcaccg agaaccagcg gaagggcaac aagacccccu acgaguacau ggggaacacg     1860

uccaucugga acgacuucga gacccgcguc cagagcaaca agaagaucac caagaagaag     1920

cagcagaagc uccugcugca gcacuucucc uacgcgcggg agcaggaguu caucgagcgc     1980

aaccucaacg acacccggua cgccacgauc uaccugagca gccugaucca gcagcaccuc     2040

aucuucuccg agagcucccg caagaagaag gugcacaccg ugagcggcau caucaccucc     2100

caccuccgga gccgcugggg guucaacaag gaccggaagg agggccacau ccaccacgcc     2160

cuggacgccg ucaucguggc ggucaccucc gaccacauga uccagcgcgu gaccaaguac     2220

uacaagcuga aggagcugaa ccgcaaccug caggccaagc ggaugccguu ccccgagccc     2280

ugggaggggu uccgccucga gcuggaggcc cggaucagcc ccaacacgca gcaguaccug     2340

aagggcuucc gcuucaagaa cuacgccgac gugaaccucu ccgagaucaa gccgaucuuc     2400

gucagccgga ugcccaagcg cuccaucacc ggcgagcugc accaggagac cauccggaag     2460

uucaucgggu acaacgagaa gggcaaggug cugaccgcca ucaagacgaa gcucgaggac     2520

auccccuucg acgcgaacgg ggacuucccc auguacggca aggagaccga ccuguacacc     2580

uacaacgcca ucaaggagcg cuuccugagc cacaagaagg acaagcggaa guccuuccag     2640

gagccgcucu acaagcccac caagagcggg ggcaucggcc cccugaucaa guccaucaag     2700

aucaucgaca cgcgcaccau cgugaacccc gucaaccagg ggaagggcgu gguguacaac     2760

agcaagaucg cccggaccga cgucuucaag aaggacgaga aguacuaccu gaucccgauc     2820

uacaccaucg accucaugaa gaacauccug ccccagaagg ccaucacggc cgggaagggc     2880

uacgaggacu ggaccaccau cgaccccucc uucaccuucc uguucagccu cuucccgaac     2940

gaccugaucc agaucgugcc cagcaagaac aagacgauca aggcgcgcac caccgugucc     3000

aagaaggagg uccugcuccc cagccugacc ggcuacuuca agggggugca cuccggcacg     3060

gccgggauca ccguggagac ccacgacggc agcgucaucg ccaacguggg guccaagcag     3120

cugcuccugu ucgagaagua ccagguggac guccugggcc acuacaccaa gaucaaggag     3180

gagaagcgga ucggcauggc caucuga                                         3207


<210>  1520
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1520
augaagaagc agccccagcg cuacauccug ggccucgacc uggggaucgc cagcgugggc       60

ugggcccuga uccucgucga cggggcggag aacccggugg gccugcugga cugcggggug      120

cggaccuucg agcgcgccga gguccccaag accggcgacu cccucgcccu ggcccggcgc      180

caggcccgga gcgugcgccg gcugauccgc cggcgcgcgc accggcuccu gcgccugcgg      240

cgccuccuga agcgggaggg cauccugucc gccgccgacu ucaacagcga cgggcucccc      300

aaccugccca ucgacgccug gcagcugcgc guggcgggcc ucgagcggaa gcugacgaac      360

caggaguggg ccgccguccu gcuccaccug gugaagcacc gcggguaccu gucccagcgc      420

aagagcgaga cccagaccga gaacaaggag cucggccggc ugcuggccgg gguggccgag      480

aacuccaagc uccugagcga guccagcgag caguaccgga ccccggcgga gcuggccguc      540

aagcuguucg ccgagcaguc cggccgccug cgcaacaagg gcggggacua cacgcacacc      600

uucaaccggc uggaccucca gaaggagcug caccuccucu ucgagcgcca gugcgcccug      660

ggcaaccccu ucgccagcgc ggagcugcag uccaaggugg acgagcuccu gaugacccag      720

cggagcgccc ugcaggggga ggccgugcuc aagaugcugg gcaagugcac cuucgagccc      780

uccgaguaca aggccgccaa gaacacguac agcgcggagc gcuucaucug gcugaccaag      840

cucaacaacc ugcggauccu guacaacggc gaggaccgcg cccucgacga gggggagcgg      900

aagcagcugc uggacgagcc cuacaagaag gccaagcuga ccuacaagca gguccgcucc      960

auccucaacc ugccggagag cgccguguuc aagggccugc gguacgggaa ggacuccgac     1020

ggccucaagg ccgagaacgg ggcgcugaug gagaugaagg ccuaccacaa gauccgcacc     1080

gugcuggaga aggccggccu caagggcgag ugggagaacc ggauccgcaa ccccgagauc     1140

cuggaccaga ucgggacggc cuucagcguc uacaagaccg acgaggacau caaggcggag     1200

cuggccgccc uccggcugcc cgagaacgug cuggacgccc uccuggccgg ccugaacuuc     1260

gacaaguuca uccagcucag ccugaaggcg cuguccaaca uccucccccu gauggagcgc     1320

gggauccggu acgacgaggc cugcaaggag guguacggcg accaccgcgc ccuggccgag     1380

agcaagaacc agaugcuccu gccgcagauc cccgccaacg agauccggaa ccccgucgug     1440

cugcgcaccc ucacccaggc gcggaaggug aucaacgcca ucauccgccg cuacgggagc     1500

ccccagcggg uccacaucga gacggcccgc gagcugggca agagcuucaa ggaccggaag     1560

gagaucgaga agcgccagga ggagaaccgg aaggagcgcg agcgggccgc cgagcacuuc     1620

aaggagcugu ucccgcgcuu caccggcgag cccaaguccg gggacauccu caagcugcgg     1680

cuguaccagc agcagcaggg caagugccuc uacagcggga uccccaucga ccugggccgc     1740

cugaacgaga agggguacgu ggagaucgac cacgcgcucc ccuucucccg gaccugggac     1800

gacagcuuca acaacaaggu gcugguccug ggcuccgaga accagaagaa gcgcaaccag     1860

accccguacg aguaccucga cggcgcccgg gacagccagc gcuggcggga guuccaggcc     1920

cgcgugcagg ggugccgguu ccccuacgcc aagaagcagc gcauccagac ggcgaagcug     1980

gacuccaaga ccgagcaggg cuuccuggag cggaaccuca acgacacccg cuacaucgcc     2040

cgguuccugu gcggguucau cgagaagucc cugcaccucc agggcgccgg caagcgccgc     2100

gucuucgccu ccaacgggca gaucaccgcc cugcugcggg gccucugggg gcugaagaag     2160

gugcgcgagg acaacgaccg gcaccacgcg cuggacgcca ucguggucgc cugcagcacg     2220

guguccaugc agcagaagau caccaaggcc augcagcgcc gggagacccu cgagaccauc     2280

aacacggaga ccggcgaggu gaagacccgc aucccccagc ccugggacuu cuuccggcag     2340

gaggucauga uccgcguguu cagcgacgcc ccgcgggagg agcuggcgga gaagcugaac     2400

ucccgccccg aggcccucca cgacuucgug agcccccugu ucgucucccg ggcccccaac     2460

cgcaagauga ccgggcaggg ccaccuggag acggucaaga gcgccaagcg gcucagcgag     2520

aacaucuccg uggugaagaa gccgcugacc gcccugaagc ucaaggacau cgagaacauc     2580

gugggcuacc ccgaccgcga gcccgcgcug uaccgggccc ugcaggagcg ccuccaggcc     2640

uacaaggacg acccggccaa ggcguucgcc gagcccuucu acaagcccgc caaggacggg     2700

ggccagggga gccuggugaa ggccguccgg aucaucgaga acaccaagca cggccugaag     2760

gugcgcaacg ggaucaccga cacgaacggc gacauggugc gggccgacgu cuaccagaag     2820

gcggacaaga agggcaagcg ccaguacuuc aucgugcccg uguacgccug gcaggucgag     2880

aagaacgagc ucccgaccgu ggacugcaag ggguacgaga ucgacgacac cuacgaguuc     2940

ugcuucuccc uguacaagua cgacaucauc gagauccaga accccgacaa caugagcaag     3000

gagcugcgcg uguacuacau caacugcgac uccagcgacg gccgguucug gcucgcccac     3060

cacgacaagu ccgccaagga gcagcaguuc cgcaucagca cccagaacca gcccaucauc     3120

aagaaguacc aggucgaccc ccuggggaag gagauccggc ugugcaaggc cgagaagcgc     3180

accgagcuca acggcaagaa gaagaaguga                                      3210


<210>  1521
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1521
augaacuacg acaucggccu gcgcaucggg aucaccagcu gcggcugguc caucaucaac       60

aaggaccucg agcggaucga ggaccugggg gugcgcgucu ucgagaaggc cgagaacccc      120

gacggcaccg ccagcgcggc cccgcggcgc gaggcccgga agucccgccg gaagcaccgc      180

cggaagaagc accgcaucga gcggaucaag cgccugaucg ugcagcacga ccuccugagc      240

aagaaggagc uggacucccu cuaccugagc cccuucaaca ucgacgugug gaaccugcgg      300

gucgaggccc ucgagcgcaa gcuggacaac cgggaguucg cccgcgugcu gauccaccuc      360

gugcagcggc gcggguucca gacgauccgg aaguccgucg agauccagga ggagggcaag      420

cugcuggaga acgugagcga gaacgagcgc aucaugaagg ugaacggcua caagaccguc      480

ggggagaugu ucuccaagca cgagaaguuc acccaccaca agcggaacaa ggacggcagc      540

uacaccuccg uggugacgcg cagccuccug cugaaggaga ucaagaccau cuucaacucc      600

cagcgcaucc ucgggaacgu cuucagcacc cagaagcugg aguccgacua ccuguacauc      660

uggggcagcc agcggcccac ccucacguac gagcagcuga uguccauggu ggggaacugc      720

aucuacgaga agaaggagaa gcgcgcgccc aagaccagcu gggccuucca guacuucucc      780

cugcuccaga agaucaacaa gcugaaggug agcgacgaca acacccugcg gaagcucagc      840

aaggaggagc gcgacaucgu caucgagcug gccuucaaga agaagaacgu guccuucgug      900

gacauccgga aggcccugaa gcucaacgac aacaccaagu ucaaccaccu gacguacagc      960

cacgucgugg agaccaagaa gguggagaag gcgaccuuca ucgagcugaa gggcuaccac     1020

cucaucaaga agcagcugaa ggaccgcaac gucgagcuga cccaggagcu ccagccgaag     1080

gacuacgacg ccaucgccgc cgccuccacg uucuucaaga acgacaucga guuccgggac     1140

uaccugcgca accaguaccu ggacagcaac ggcaagcgga aguccaacau cgcgaacaag     1200

guguucgagg acaaggugau cgccgccguc uucgagcuca acuucagcaa ggugugccac     1260

cuguccuuca agagccugua caagcucauc ccccaccugg agaaggggcu ggacuacaag     1320

gaggccaucc ucugcgccgg cuacaacuuc cgcgacgaga acaagcggga caagaagcug     1380

uuccugcccg ucauccagca ggagaccaac cccguggugc accgcgcgcu cacccaggcc     1440

cggaagguca ucaacgccau cauccgcgag uacggguccc cgaacaccau ccgguucgug     1500

guggccaacg accuggccaa gacguacaag cagcgcaagg agcuggagag cacccugaag     1560

aagaaccggg cggagaacga gaagaucaag accaagcucc uguccauggg caucaucaac     1620

cccaccggca uggacaucgu caaguacaag cugugguacg agcagagcgg gaucugccuc     1680

uacacgggcc gccagaugga gcuguccagc cuguucaagc ccggguacuc caucgugaac     1740

cacaucaucc ccuacaaccg gagcuucgac gacaccuacc acaaccgcgu gcucacccug     1800

accgagacga aguacgagaa gggcaacaag aucccguucg aguacuucgg ggccgaggag     1860

accugcugga acgaguacga gaagcgcguc uccaccagca agaagaucca cgacaagaag     1920

cgggagaagc ugcucauccg caacuucgac aucgaguacg agaacgagau gaucuccaac     1980

aacguguucg acacccacua caucggcagc uacuucgaga aguacaucga cgagcaccuc     2040

caguucaaga aguccacgaa gaagcagaag guguucucca uccacggcaa cauggucgcc     2100

caccuccaca gccggugggg guucaacaag aacucccgcg agaucuaccu gaaccacgcc     2160

guggacgccg cgaucguggc cguccugacc gaccagcuga uccggaacau cgccgacuac     2220

uaccgccggg acaacauccu ggagcagaag ggcagccuga aguuccccga gcccuggaag     2280

uccuucagca ucgagcugga ggcccgcauc agcccccagg ugcgggaccu caucaagaag     2340

cugaaccugg acagcuaccu cgacguggac auccugcaca ucaagccggu cuucaucucc     2400

aagaagcccc gccggaaggu gaacggggag gcgcacaaga gcaccauccg cucccugauc     2460

ggcaucaacg agaucgggaa gaccgugacg ugcaucaaga cccggcucga ggacaucccc     2520

uucgacgaga acggcgacuu ccccauguac ggcaaggaca ccgacaagua caccuacacc     2580

gccaucaagg agcgcuaccu ggaguucaac aaggacaagg agaaggccuu cagcacgccg     2640

cuguacaagc ucaagcggaa cggggagccc ggcaaccaga ucaaguccgu caagaucauc     2700

gacacccgca acaucgugaa caaggugaac caggggaagg gcaucgccua caacagcaac     2760

aucauccggg ucgacguguu cucccgcgac cagaaguacu acgugguccc caucuaccug     2820

cgggacuuca ucaaggggag ccugcccaac aagaucauca ccgccgcgaa gggcuucaug     2880

caguggccgg agaccgacaa cuccuacacg uuccucuuca gcaucuuccc caacgaccug     2940

aucaaggugg ugcccaagga gggcaaguuc aucaaggcca agaacgagaa caaggagaac     3000

aucaaccuga ccgagauccu cggguacuuc aagggccugg acagcuccac cggggccuuc     3060

acccuggaga gccacgacgg cucccuccug gcccgcggca ucgggacgaa gaaccugcuc     3120

cugaucgaga aguaccaggu cgacgugcug ggccgguaca acaagguggg gaacgaggag     3180

cgccaggaca ucaagaucca gcagaacuga                                      3210


<210>  1522
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1522
augaacuacg acaucggccu gcgcaucggg aucaccagcu gcggcugguc caucaucaac       60

aaggaccucg agcggaucga ggaccugggg gugcgcgucu ucgagaaggc cgagaacccc      120

gacggcaccg ccagcgcggc cccgcggcgc gaggcccgga agucccgccg gaagcaccgc      180

cggaagaagc accgcaucga gcggaucaag cgccugaucg ugcagcacga ccuccugagc      240

aagaaggagc uggacucccu cuaccugagc cccuucaaca ucgacgugug gaaccugcag      300

gucgaggccc ucgagcggaa gcuggacaac cgcgaguucg cccgggugcu gauccaccuc      360

gugcagcgcc ggggguucca gacgauccgc aaguccgucg agauccagga ggagggcaag      420

cugcuggaga acgugagcga gaacgagcgg aucaugaagg ugaacggcua caagaccguc      480

ggggagaugu ucuccaagca cgagaaguuc acccaccaca agcgcaacaa ggacggcagc      540

uacaccuccg uggugacgcg gagccuccug cugaaggaga ucaagaccau cuucaacucc      600

cagcgcaucc ucgggaacgu cuucagcacc cagaagcugg aguccgacua ccuguacauc      660

uggggcagcc agcgccccac ccucacguac gagcagcuga uguccauggu ggggaacugc      720

aucuacgaga agaaggagaa gcgggcgccc aagaccagcu gggccuucca guacuucucc      780

cugcuccaga agaucaacaa gcugaaggug agcgacgaca acacccugcg caagcucagc      840

aaggaggagc gggacaucgu caucgagcug gccuucaaga agaagaacgu guccuucgug      900

gacauccgca aggcccugaa gcucaacgac aacaccaagu ucaaccaccu gacguacagc      960

cacgucgugg agaccaagaa gguggagaag gcgaccuuca ucgagcugaa gggcuaccac     1020

cucaucaaga agcagcugaa ggaccggaac gucgagcuga cccaggagcu ccagccgaag     1080

gacuacgacg ccaucgccgc cgccuccacg uucuucaaga acgacaucga guuccgcgac     1140

uaccugcgga accaguaccu ggacagcaac ggcaagcgca aguccaacau cgcgaacaag     1200

guguucgagg acaaggugau cgccgccguc uucgagcuca acuucagcaa ggugugccac     1260

cuguccuuca agagccugua caagcucauc ccccaccugg agaaggggcu ggacuacaag     1320

gaggccaucc ucugcgccgg cuacaacuuc cgggacgaga acaagcgcga caagaagcug     1380

uuccugcccg ucauccagca ggagaccaac cccguggugc accgggcgcu cacccaggcc     1440

cgcaagguca ucaacgccau cauccgggag uacggguccc cgaacaccau ccgcuucgug     1500

guggccaacg accuggccaa gacguacaag cagcggaagg agcuggagag cacccugaag     1560

aagaaccgcg cggagaacga gaagaucaag accaagcucc uguccauggg caucaucaac     1620

cccaccggca uggacaucgu caaguacaag cugugguacg agcagagcgg gaucugccuc     1680

uacacgggcc ggcagaugga gcuguccagc cuguucaagc ccggguacuc caucgugaac     1740

cacaucaucc ccuacaaccg cagcuucgac gacaccuacc acaaccgggu gcucacccug     1800

accgagacga aguacgagaa gggcaacaag aucccguucg aguacuucgg ggccgaggag     1860

accugcugga acgaguacga gaagcgcguc uccaccagca agaagaucca cgacaagaag     1920

cgcgagaagc ugcucauccg gaacuucgac aucgaguacg agaacgagau gaucuccaac     1980

aacguguucg acacccacua caucggcagc uacuucgaga aguacaucga cgagcaccuc     2040

caguucaaga aguccacgaa gaagcagaag guguucucca uccacggcaa cauggucgcc     2100

caccuccaca gccgcugggg guucaacaag aacucccggg agaucuaccu gaaccacgcc     2160

guggacgccg cgaucguggc cguccugacc gaccagcuga uccgcaacau cgccgacuac     2220

uaccggcgcg acaacauccu ggagcagaag ggcagccuga aguuccccga gcccuggaag     2280

uccuucagca ucgagcugga ggcccggauc agcccccagg ugcgcgaccu caucaagaag     2340

cugaaccugg acagcuaccu cgacguggac auccugcaca ucaagccggu cuucaucucc     2400

aagaagcccc ggcgcaaggu gaacggggag gcgcacaaga gcaccauccg gucccugauc     2460

ggcaucaacg agaucgggaa gaccgugacg ugcaucaaga cccgccucga ggacaucccc     2520

uucgacgaga acggcgacuu ccccauguac ggcaaggaca ccgacaagua caccuacacc     2580

gccaucaagg agcgguaccu ggaguucaac aaggacaagg agaaggccuu cagcacgccg     2640

cuguacaagc ucaagcgcaa cggggagccc ggcaaccaga ucaaguccgu caagaucauc     2700

gacacccgga acaucgugaa caaggugaac caggggaagg gcaucgccua caacagcaac     2760

aucauccgcg ucgacguguu cucccgggac cagaaguacu acgugguccc caucuaccug     2820

cgcgacuuca ucaaggggag ccugcccaac aagaucauca ccgccgcgaa gggcuucaug     2880

caguggccgg agaccgacaa cuccuacacg uuccucuuca gcaucuuccc caacgaccug     2940

aucaaggugg ugcccaagga gggcaaguuc aucaaggcca agaacgagaa caaggagaac     3000

aucaaccuga ccgagauccu cggguacuuc aagggccugg acagcuccac cggggccuuc     3060

acccuggaga gccacgacgg cucccuccug gcccggggca ucgggacgaa gaaccugcuc     3120

cugaucgaga aguaccaggu cgacgugcug ggccgcuaca acaagguggg gaacgaggag     3180

cggcaggaca ucaagaucca gcagaacuga                                      3210


<210>  1523
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1523
auggccaccc gccugggccu cgacaucggg accaacagca ucggcuggug ccuguacgac       60

ggggacucca uccgggacau cggcgugcgc aucuuccugg acgggcggga gcccagcaag      120

cucacggucu ccggcagcgg caccccgcug gccgugaagc gccgggacgc gcgcggggcc      180

cggcgccggc gcgaccggua caucggccgc cgguccgcca ugcugcgcga gcuccggaag      240

cacgggcugc ugcccgccga ccccaagcag gccaaggugc ucgagaccca ggaccccuac      300

gcgcugcgcg uccgggcccu ggacgagaag cucgagccgc accagaucgg ccgcgcccug      360

uuccaccuga accagcggcg cggguucaag agcaaccgga aggccgagcg cgcguccaag      420

gccggcgagg acggcaagau cgccaccgcc acgaagcucc uggacgagga gaugcagcgc      480

cugggggccc ggacccucgg cgaguuccug gugaagcgcg acagcgaguu cgacggggag      540

gcgggcaagc gggugcgcau gcgggccgac uccaaggccu acgacuucua ccccgaccgc      600

cggcacgucc uggacgaguu ccgcgccauc uggaacgccc agaaggugug gcaccccgag      660

cuccugaccg agcaggcgug gaacgacauc uuccggguga ucgaguacca gcgcgagcug      720

aagccccagg ucgugggcca cugcaccuuc gccgggauca acggcgugcc ggccgacgag      780

aagcggcucc ccaaggccca cccccuguuc cagcagcgcc ggcuguacga ggaggucaac      840

cagcucaagg ugguggcccc cggggaggcg ggccgcccgc ugacgcggga cgagcgcgac      900

gcccugaucc ucaagcugca ggacaagaag uccgucagcu ucgagacccu ggggaagacc      960

cucaagcuga aggacggcga gcgguucaac aaggagagcg agaaccgcaa ggcccuggac     1020

ggcgacgagg ugcgggccgc ccucuccggg aagaagcugu ucggcacccg cugguuccac     1080

uucagcgcgg aggagcagug gaugaucauc gagcgccugc ucgaggagga ggacuccgag     1140

acgcugcuga ccuggcucaa gaucggguac cggcuggacg acgaggccgc ccuggccgug     1200

gcgaacgcca agcuccccga gggccacggg cgcuucggcc ugagcgccac caccgcccug     1260

cucaaggagc ugaaggccga cgucugcacg uacgcggagg ccgccaagcg ggccggcuuc     1320

caccacuccg acuuccgcac cggggaggug uucgaccagc ugcccuacua cggcgagauc     1380

cuggcccggg agaucgcgcc cgggaagccg gaguacggcg acgagaacga gcgccgguuc     1440

gggaagauca ccaaccccac cgugcacauc ggccucaacc agcugacgaa gcuggucaac     1500

gccaucaucc gccuccacgg caagcccgac uacaucuucg uggagcuggc ccgggagcug     1560

aagcucaacg agaaggagaa gaucgccuac aaccgccagc ugaaggagaa caccgacgcc     1620

gcgcgggacc gcggggccaa gcugggcacc augggggugc gggacaccgg ccgcaaccgg     1680

cucauccugc gccuguggac ggagcucaac cccggcgacg cccugaaccg gcgcugcccg     1740

uucugcgggg agcagaucag ccagcggaug auccagaacg gcgaggucga gaucgagcac     1800

aucauccccu acucccagug cuucgacgac gggaucggca acaaggugcu gagccacguc     1860

cgcugcaacc ggcagaaggg ggaccagacc cccugggagc gcuggggcca cgaccccgac     1920

cgcuggagca ucauccagga gcaggucgcc caccuccacc gguccaagca guggcgcuuc     1980

ggcccggacg cgcgggaccg cuacgccgcc gaggaggaca gcuuccugcc ccggcagcug     2040

aucgacaccc aguaccucac ccgcaucgcc gccaaguacc uggcgugccu guaccccgag     2100

cgcggggagc ggcacguguu cgccgugcac ggcgggauga cggccaugcu ccggcgccug     2160

ugggaccuga acuccauccu ccuggaccac aacugggugg agaaccugaa cagcaacgcc     2220

cccaagaacc ggcucgacca ccgccaccac gccaucgacg cggccguggu cggcaucaug     2280

accccggggc ugaucgccga gaucgccaag gccgcggccc gggccgagaa gcagaaccug     2340

gaccgccucu ucgaggagcu gcccaugccc ugggcccagc acccgaacga ccggcgcuuc     2400

cgggacgagc ugcgcgagaa gauccucgcg gccaccgugu cccacaaggc cgaccacggc     2460

cggcagggca agcccgagcg cgggcgcgac aucaccgccg gcaagcugca caacgacacg     2520

gccuacgggu ucaccggccu gaccagcgac accgggcucc ccguggucgu gcaccgggug     2580

ccccugacgu cccugacccc gaaggacauc gcggaccccg accgcauccc cgacaccacc     2640

cuccggggca gccuguggca ggccacccgc gaccuguccg gcaaggaguu cgagaaggcc     2700

cuccuggacu ucagccggac gcacgcccag uucaagggga uccgccgggu ccgcgugcgg     2760

gagggccuga cccucauccc cauccgcaac cacgccggga agccguacaa gggcuaccug     2820

ggggacucca acgcgcggua caacgugugg cagaugcccg acggccgcug gguccaccag     2880

gugaucagca ccuucgaccu gcaccagccc ggcuucaucg agcagcggcc ccacccggcc     2940

gccaagaagg ugcucucccu gcgccagaac gaccugaucg ccaucgagcg ggacggggcc     3000

cuccgccaga ucaugcgggu ccagaaguac ggccagaacg ggcagaucuu ccugaucgag     3060

cacaacgagg cgggcaacau ggccgagcgc aacaagaaca ccgacgaccc cuucaaguuc     3120

uacggccccg gggccagcgc ccugcggaac augaaggccc gccaggugcg gaucgacgag     3180

cucggccgca ucuucgaccc cgggccgcgc uga                                  3213


<210>  1524
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1524
auggccaagg acuucgagua cauccugggc cucgaccugg gggagaccag caucggcugg       60

uccguggucc gccugaacga gggggugccc gagcaccucg agcggcuggg cgugcgcauc      120

ugcagcgacg ggcgggacgc ccagucccac gagccgcuga gcgucgcgcg ccggaucgcc      180

cgcggccucc ggcgccggcg cgaccgguac auccaccgcc ggauccgccu gaccgacgug      240

cuggugaagu acggccucau gcccgaggac aucaaggagc ggaagaagcu gcaggcccug      300

aaccccuacc gccuccgguc cgaggcccug gaccgcaagc uggagcccua cgagcucggg      360

cggguccugu uccacaucaa ccagcgccgg ggcuucaaga gcaacaugaa gacggaccgc      420

aaggacuccg agagcggggc caugaagcag gcgauccuga agacccgcgc caagauggag      480

gccuccggcg cccggacccu cggggaguuc cuguacuucc ugaacaagga caagaccagc      540

acgcaggagu acgugccggu gcgcaugcgg ucccacagcg agaagaacaa gaccgucuac      600

gagauguacc ccgaccgcgc gauguacgag gacgaggugc acaagaucuu cgagcagcag      660

gagcucuccc ccgaggccca gaccgagauc uucaacacca ucuucuucca gcggccccug      720

aagccggugg agcugggcuu cugcgaguuc gaggacggcg agcgccgggc cuacacggcc      780

agcgucgugu uccagaaguu ccgcgcccuc cagcagauca accagaucua cgugcaggac      840

gacuacgagc ggcgcccccu gaccaaggag gagaaggaca uccugaagca cuuccuccug      900

gaggacuucu ccgcgcugga caagaacggg cuccugaccu gggccaaggu ccggacccug      960

cucaagcugc agaagaagaa guucaaccug gagagcgagc gccggaaggg ccucaacgcc     1020

gacgugacgu ccuggaagcu gagcaacgcc gccugcuucg ggcgcgagug guucuccuuc     1080

agcgacgcga agaaggagga gaucguggcc cugaucaccg gcgccgagaa ggaggaggag     1140

cucaucgccg agcugaagac caaguacggc cugagcgacg agcgggccaa gaacaccgcg     1200

aacgucuccc uccccgacgg gguggccagc cuguccgcca aggccaugaa gaagcugauc     1260

cccuuccucg aggagggcau gcuguacgac gacgccguga agcaggcggg guacgucuuc     1320

agcuccagcu ucaagggcgu gcugccggag aaguacgaga cguucaucga gcccgagucc     1380

ggggagugcu ucgacgagcu ccccuacuac ggccaggugc ugcgccggua cguccugggc     1440

ggggugccca gcgaggagaa caagcuguuc ccggagaagu acuacggcaa gaucaacaac     1500

cccaccgucc acaucgugcu caaccagcug cgcaagcuga ucaacucccu cguggccaag     1560

uacgggcacc cccggaagau cgucguggag auggcccgcg accugaagcu gagcaagaag     1620

cagaaggacg ccaucgcgaa ggagcaggcc cucaacgagc gcgagaacaa gcggaucggc     1680

gccgagcugg agcgccugga cauccggaac aacuacgaca accgcaugaa guacaagcuc     1740

ugggaggacc uguccaccga ccccaccaag cggagcugcc cguucugcgg gaagcagauc     1800

ucccagcagg acaucuucag ccccgaguuc gagaucgagc accugauccc cuucucccgc     1860

agcuucaugg acggccgguc caacaaggug cucagcugcc gccggugcaa ccgcgagaag     1920

ggcaacaaga cgcccuucga ggccuucggg aacgacucca cccgguggaa cgccaucaug     1980

ggccgcgucg agagccugcc ggagaacaag cgguggaagu ucgcgagcga cgccaugucc     2040

cgcuucgagc gggacgggga ccugcucgcc cgcaugauga ccgacaccca guacauggcc     2100

cggaucgccc gccgguaccu gugcugcgcg gccaacccca accaggugua cggcaucccc     2160

gggcagcuga cggccaagcu ccgcgagcac uggggccuga acagcauccu guccgaccag     2220

aaggagaagg accggagcga ccaccgccac cacgccaucg acgccuucgu ggucgcgugc     2280

accgaccgcg gcacccucca gaaguacucc accgagcggc gcgacuacgc caagcccgag     2340

augccgagcg agaaggcgcc ccacaucccc uucgccgacu uccggcuguc cgacaucaag     2400

cgcguguucg acgagaucau cgugagccac aagccggacc gggggaacgc ccgcgccgcc     2460

auccugaagg gcgggacggu cgccaagcuc cacgaggaga ccuacuacgg caugggcggg     2520

gagggcucca agaaggggac cuggaagcug auccagcgga uccccgugag cgagaucaag     2580

aaggccuccg accuggacug ccucaucgac cagaagggca ccgcgaaccc ccugcgcgug     2640

cugcucgucg gggccagcga guccgagcag gagcagauca ucgccagcua cuucgcccgg     2700

cgcggcacgc acaaggugcg gaccuaccgc gagcggaaca aggacgugau ccugcccuuc     2760

aaggacaagg acggcaagcu guaccgcuac gccgucuacg gguccaacgc guacgccgag     2820

aucuacugcc cggaccgggg caagaacgcc gggaaguggc aggucgagau cauccccaac     2880

uacugcgccc accagaaggg cuucaucccc aaguggcgcc acaccgacgc ccacgcgaag     2940

cucaucaugc ggcuguaccg caacgacaug guggccuacg agaaggaggg gaagaccgug     3000

aucgcccggg ugaagaagau gggcggcaac ggggugaucg ucuuccgcaa ccaccugauc     3060

gccaaggagg agggcgaccg ccucagcugg caggcggugg uguccgggau gcagagcgcc     3120

aaccugcgga agaucuacgu cgacgugcug ggccgcguga ucgaccccaa gcggccgaug     3180

acgcagaacg agaccgacgg gcaggacaac uga                                  3213


<210>  1525
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1525
augagccacu uccgccugaa gcagcucaac uacauccugg gccuggaccu cgggaucgcc       60

uccgugggcu gggccgucgu ggagaucgac accgaggaga accccacccg gcugaucgac      120

gugggggucc gcacguucga gcgggcggag gugccgaaga ccggcgacag ccuggccgcc      180

gcccgccggc aggcccgcuc cgcgcggcgc cucauccggc gccggaccca ccgccugcug      240

cgggcccgcc ggcuccugaa gcgcgagggg auccugcacg ccgacgaccu caacgccgag      300

ggccugguga aggagcugcc caacaccccc uggcagcucc gggucgacgg ccuggaccgc      360

aagcugaugc ccaaggagug ggcggccgug cuccugcacc uggugaagca ccggggguac      420

cucagccagc gcaagaacga guccgagggc gccgacaagg agcuggggcg gcugcucgcc      480

ggcguccacg agaaccacca ggcccugcag agcuccgagu accgcacgcc ggcggagcug      540

gccgugaaga aguuccaggc ccagagcggg cacauccgca accagcgggg cgacuacacc      600

cacaccuucg accgcaagga ccuccagcag gagcugcacc agcuguucgc cgcccagcag      660

aaguacggca accccuucgu guccgacggg cucaagcagg gcaucgagac ccugcugaug      720

cagcagcggc ccgcgcucag cggggacgcc guccagaaga ugcugggccg cugcacguuc      780

gagaccgagg aguucaaggc cgccaagcac accuacuccg ccgagcgguu caucuggcug      840

accaagcuca acaaccugcg cauccaggag aacggcaacg agcggugccu gacggcgcag      900

gagcgcgggc agaucaucga ccuccccuac aagaaggcca agcugaccua cgcccaggug      960

cggaaggagc ugagccuccc ggagaacgcc uucuucaagg gccugcgcua cgggaaggag     1020

aacgccgaga ccgcggcccu gauggagaug aaggccuacc acgccauccg gaaggcgcuc     1080

gagaaggccg gccugaagac cgaguggcag gggcuggccu gcaagcccga gcuccuggac     1140

gccaucggca cgguguucuc ccuguacaag accgacgagg acaucagcac ccgccugcag     1200

ggccagcucu ccgaggacgu caucaacgcc cugcuggcgg ggcucaacuu cgacaaguuc     1260

auccagcuga gccugaaggc ccucuccaag auccugcccc ugauggagca gggccagcgg     1320

uacgacgagg cccacgccgc ccucuacgug cgcagcacgc cccagccgca gcggcugcug     1380

ccccagauca ccgcggacga gaucugcaac cccguggucc uccgcacccu gucccaggcc     1440

cggaagguga ucaacgccau cgugcgccgg uacgggagcc ccgcccgcau ccacaucgag     1500

accggccgcg aggucggcaa gagcuucaag gaccggcagg agauccagaa gcgccaggag     1560

gacaaccaca aggagcggaa gcaggccgcg gagaaguucc uggaguacuu cccgaacuuc     1620

guggggcagc ccaaggccaa ggacauccuc aagcugcgcc uguaccagca ccagcacggc     1680

cagugccucu acuccgggaa gacccuggac aucugccggc ugaacgagaa gggcuacgcc     1740

gagaucgacc acgcccuccc cuucagccgc acgugggacg acuccuucaa caacaaggug     1800

cugguccugg cgggggagaa ccagaacaag ggcaaccaga cccccuacga guggcucggg     1860

ggcgccagca acuccccgcg guggcaggag uucguggccc gcgucggcag cucccgguuc     1920

caccacagca agaagcagcg cauccugcug cggcagcucg acgaggaggg guucaaggag     1980

cgcaaccuga acgacacccg guacguggcc cgcuuccugu gcaaguucau cgaggagaac     2040

augcuccugc ugggccgggg gaagcgccgg guguucgccu ccaacggcca gaucaccagc     2100

uuccuccgcu cccggugggg ccugcgcaag guccgggagg agaacgaccg ccaccacgcg     2160

cuggacgccg ugguggucgc cugcagcacg guggccaucc agcagcgcau caccuacuuc     2220

gugcggcagc gcgagaugaa caucuucgac ggggagcuca ucgacaagga gaccuccgag     2280

gucagccggc ccuacuuccc ccagcccugg gacuucuucc gccaggaggu gaugaugcgg     2340

guguucggca agccggacgg ggaccccucc uuccccgagg ccgacaccaa ggagacgcug     2400

ugcacccugc ucgagacccg ccugagcucc cggccccagg cgguccacga guacgugacc     2460

ccgcuguucg ugagccgcgc ccccacgcgg aagauguccg gccaggggca caaggagacc     2520

gucaagagcg ccaagcuccu gcacgagggc aagagcgugc ugcgcgugcc ccucacccag     2580

cugaagccga accugcucga gaagaugguc aaccgggacc gcgagcccca gcuguacgag     2640

gcccugaagg agcggcucgc cgcguacggc aacgaccccg ccaaggccuu cgccaccgag     2700

uuccgcaaga agggcgggca gcuggugaag gcggugcggg ucgaggacac ccagaagucc     2760

ggggugcugc uccacaaggg cacgggggug gccgacaacg ccaccauggu ccgcguggac     2820

guguucgaga aguccggcaa guacuaccug guccccaucu acagcuggca ggucgcccag     2880

ggcauccugc cgaacaaggc cgugauccag gggaaggacg agaagaacug ggacgagaug     2940

gacgagaccu acaccuucaa guucagcaug uaccccaacg accucgugga gaucaacacg     3000

ggcaagcagc ggcuguucgg guacuacauc ggccuggacc gcgcgaccgg ggccaucucc     3060

auccgggagc acgaccucga caacuucaag ggcaagaacg gcaucuaccg cgugggggug     3120

aagaccgcca ucagcuucaa gaaguaccag accgacgagc ugggcaaggu cguggugccc     3180

ugccgcccca agaagcggcu gccgguccgc uga                                  3213


<210>  1526
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1526
augcgccugg gccucgacau cgggaccaac agcaucggcu gguggcugua ccagaccgac       60

gacgggacga ucaccgacgu gcuggacggc gggguccgga ucuucuccga cggccgcgac      120

ccccagagca aggagucccu cgccguggac cggcgcgugg cccgggcgca gcgccggcgc      180

cgggaccgcu accugcggcg caaggccgcc cugaugaagc ggauggccgg cgccgggcuc      240

augccgaccg accaggcgca cgccaagaag cuggagagcc uggaccccua cgcccuccgc      300

gccgagggcc uggacugcga gcugccccuc acccaccugg ggcgggcgcu guuccaccuc      360

aaccagcgcc ggggcuucaa guccaaccgc aagacggacc gcggggacaa cgagagcggc      420

aagaucaagg acgccaccgc ccggcuggac ugggccaugc gggacgccaa ggcgcgcacc      480

uacggcgagu uccugcacac ccgccagcag aaggccgacg accccaagcg gaugccgacg      540

guccgcaccc ggcucaccgu ggcccgccgg gacaacgccg agaaggagga ggccggguac      600

gacuucuacc ccgaccgccg gcaccugucc gaggaguucg acgcgcugug ggccgcccag      660

gccgacuacc acgaggcccu cagcgacgac cugcgcgacc agaucaagac caucaucuuc      720

caccagcggc cccugaagac gcccgaggug ggccucugcc uguucaccga cgagcgccgg      780

aucccguccg cgcacccccu gaaccagcgc cggauccucc uggagaccgu caacgcccug      840

cgcaucgugg cccgcgggga ggccgcccgg cggcucaccc uggaggagcg cgaccagauc      900

gugcacgcgc uggacaacaa gaaccacacg aagacccuca gcggcauggc caugaagcug      960

aaggcccugg gcaaggucau ccggcuccgc uccgaccaga ccuucacccu ggagacggcc     1020

aaccgcgacg cgaucgccug cgaccuggug cgggccagcc ucucccaccc cgaccgcaug     1080

ggcggggugu ggaccacccu ggacgagggg gcccaguggg aggucgugca gcggauccgc     1140

gccgugcaga gcgacuccga guucgacgcg cuggucgugu ggcucgagca gaaccacggc     1200

cuggacaacg cccacgccca ggccaccgcc aacgcgcccc ugccggaggg guacggccgg     1260

augggccuca uggccacgcg ccggauccug gccgcccugc agagcgacgu gaugcccuac     1320

uccgacgccg ucgcggccug cggguggagc cacuccggcg ggcccaccgg cgaggugcuc     1380

gagaagcugc ccuacuacgg gaagauccug gaccgccacg ugaucccggg caccuacgac     1440

gagaacaacg aggacgucaa gcgguacggc cgcaucacca accccacggu gcacaucggg     1500

cugaaccaga uccggcgccu cgugaaccgg aucaucugca uccacggcaa gcccgacgag     1560

aucgucgugg agcuggcccg cgaccugaag aacagcgagg accagaagcg ggagauccag     1620

aagaucauca agaagaacac cgacgccgcg aucaagcgcg gggagaagcu caucaacgag     1680

cugggccaga aggacaccgg ggccaaccgg cugguccucc gccuguggga ggaccugggc     1740

cacgacguga ugacccggaa cugccccuac agcggcaagc gcaucuccgc cgggaugcug     1800

uucgacggca gcugcgacgu ggaccacauc cucccguacu cccggacgcu ggacgacggg     1860

aucgccaaca agacccucug ccugaaggag gccaaccgcc agaaguccaa caacaccccc     1920

ugggaggucu ggggcgagac cgagcagugg gacacgaucg cggccaaccu gaagaaccuc     1980

cccgacaaca aggccuggcg cuucgccagc gacgccaugc agcgguucga gggcgagaac     2040

gacuucagcg cgcgcgcccu gaaggacacc caguaccugu cccggaucgc ccgcgccuac     2100

cucgacgccc uguacgacgg gggcgacggg aacagccacg uguggguggu ccccggccgg     2160

cugaccgaga ugcuccgccg gcacuggggg cugaacggcc uggaggcgcu caccgacucc     2220

gacgagaaca ccgugaaggc caagaaccgc auggaccacc ggcaccacgc caucgacgcc     2280

gcgguggucg ccgccacgga ccgcggccug auccagcgga ucagcaagau cgccaagcgc     2340

gacgagcagg ccggggcgga ggagguggcc cgguccguga gcccgcccug ggacggcuuc     2400

cgcgacgaca ucgccgccca gcugucccgg aucaucguca gccaccgcgc cgaccacggg     2460

cggaucgacu cccaggcgaa gcagcagggc gaggacgcca ccgccgggca gcuccacaug     2520

gacaccgccu acggcguggu ggacgccggc cgcgucguga gccggaagcc ccugcugucc     2580

cucagcgagu ccgacaucaa gaagauccgc gaccccgacc ugcagcgcca ccugacccgg     2640

gugacgaagg ggcucgacaa gaaggaguuc gagcacgcgc ugagcgacuu cgccaacgcc     2700

cgcaagcugc cggaccagag cgagaacccc uacuucggca ugcggcgcgu ccggcucauc     2760

gacgcccugc aggacuccgc ccgcgugagc gugcgggacc gcgacgggca caucuacaag     2820

gcguacaagg ccggcuccaa ccacugcuac gaggucuggc ggcugcccga cgggaagauc     2880

aagccguggg ccaucagcac cuucgaggcc caccagaccg gcaccgaguc caagccccac     2940

cccgcggcca agcgccuccu gcggguguuc aagcgcgaca ugguggccau cgagcgggac     3000

ggcgagacgc ugaugugcua cguccagaag auggacaucg ccaacggccu cuuccuggug     3060

ccccacaccg aggggaacgc cgacgcgcgc aaccgggaca aggacgacag cuucaaguuc     3120

auccagaugu ccgccgugag ccugauccgc gccaagaucc ggcgcaucca cgucgacgag     3180

auggggcggc uccgcgacca gggcccgccc cgguga                               3216


<210>  1527
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1527
augcgcuaca ccaucggccu ggacaucggg aucgccagcg ugggcugggc cgucaucaac       60

gaggacaagu cccggaucga ggaccucggg gugcgcgugu ucaagaaggc ggaggagccc      120

gacggcaaga gccugaaccu ggcccggcgc gaggcccggg ggucccgccg gcgcauccgg      180

cgccgggcca cccgcaugca gaaggucaag gagcuccugg ugaaguacaa ccuggugagc      240

gagaaggagc ucgagacgcu guacaucauc accgagacca ccccggacgu cuacgagcug      300

cgggccaagg gccucgaccg ccugcugucc cggcaggagu ucgcgcgcgu gcucacgaac      360

aucgccaagc ggcgcggcua caagagcaac cggaagaucg acgaggagga caaggaggug      420

gggaagcugu ccaagggcac caucgagaac aagcgcaucc ugcaggagaa gggguaccgg      480

accaucggcg agauguucua caaggacgag aaguucgagu acaacaagcg caacaaggcc      540

ggggaguaca acagcaccgu ccuccgcucc augcugaucg acgaggugca cacgcuguuc      600

gagaugcagc ggaaguucaa gagcgaguuc gccaacgagg aguucgagaa ggaguacgug      660

gaggucauga ccuaccagaa gcccuacauc acccccgagc uccuggagaa gaugcugggc      720

aagugcaccu ucgagaaggg cgagccccgc gcgccgaaga acuccuacac guucgagcgg      780

uucaugcucc ugcagaaggu gaacaaccug aacaucuaca ucaacgggga gaagguggag      840

aucccccagg agaagaagaa ggagaucauc gagcucgccu acaagcagag cgagaucaag      900

uacacccagc ugcgcaagaa gcuggagcuc cccaucgagg cccgguucgc cgaccugaac      960

uaccagauca agcgcaccga ggacgagucc gaggaggccc uggucaagaa gaucgaggac     1020

aagaaguucg ugaagcucga gggcuggcac aagauccggc uggcgcugaa ggagaacaag     1080

gacaaguucg agaagaucga gaagaacccc gagcuccaga acaucaucgc cgacgcccug     1140

gugcgcaaca agaccgacga gacgaucaag aacuaccugg agaaguccga caucgacgag     1200

gagaucauca acgccauccu caacaucaac uucaccaagu ucgggcaccu gagcuacaag     1260

gccaugaaca agaucauccc ggagcuggag aagggccuca ccuacgacaa ggcgugcgag     1320

aacgucggcu acgaguucaa ggggcggaag aacgagcugc aguacaagcu gccgcccgug     1380

accgagcucg aggacgagau ccugaacagc gugguccugc gcgccguguc ccagacgcgg     1440

aaggugauca acgccaucau cgacaaguac ggcagcccca ccgccaucua caucgagacc     1500

gcccgcgagc ucuccaagcc cuacgacgag cggaugaaga ucgagaagcg ccagaaggag     1560

aacgcggcca acaacgacca gaucaaggag uacaucaagg agaccuucgg guucgacccc     1620

aagcccuucg acaucgucaa gaugaagcug uggcgggagc agaacggcaa gugcgccuac     1680

agcagcaagc ccaucccggc cgagcgccug uacgaggaga acuucgugca ggucgaccac     1740

aucauccccu ucucccggug cuucgacgac agcuaccaga acaaggugcu cgugcugacg     1800

gacgagaacc agaagaagaa ggagcgcacc cccuucgagu acuucgggga ggacaaggag     1860

cgguggcacc aguucgagga guucgucaac cugaccuaca aguucaacac ccgcaagaag     1920

gagaaccugc ucaucaagaa cuucaacgag gagaagucca aggaguggau ccagcgcaac     1980

aucaacgaca cgaaguacau cagcaaguuc auguacaacu acaucgccaa caaccugaag     2040

uucgcggacu ccgagagcaa gcggaaggug uacaacauca acggccaggc caccuccauc     2100

cugcgccacu acuggggccu caagaaggac cgggaggaga gcgacaagca ccacgcccag     2160

gacgccguga ucaucgcgug cgccaccaac aagaacauca agaaggucuc cgacuacagc     2220

cgccggaagc ugcuguacaa gaacaacgag aucaucgacg aggagaccgg ggagaucauc     2280

gacaagaagu acaacaucga caucuccauc aaggagcccu ggccgaaguu ccgcgaggag     2340

guggaggccc ggauggagga cgccgacaac aacggccagc ucuacgcccu gaucaacggg     2400

aacuucacga acuacgacga cguggacauc acccacauca agcccaucuu caccagccgc     2460

augcccgagc ggaagaucac cggccgcgcg cacaaggaca cgaugcgguc ccagaaguuc     2520

auccagcagg ggcagaacuu caccgucgug aagaagcccc ugaaggccau cagcaagcag     2580

gagaucgagg gcaucaucaa caacccggac uucaagaacc ucuaccuguc cgacaagaag     2640

cuguacgacg acaucuacca gaagaugaag gaggccgacu ucaaggccga gaaggccuuc     2700

gcgaacgagu accgcaagua cagcaagaac ggcaacuccc ccaucgugcg gagcaucaag     2760

gucgccuccg ccggggccac cggcgugcag cucaagaacg gggccaucgc ggagaacgcc     2820

aacauggugc gcgucgacgu guucgagaag gacaacaaga acuaccuggu gcccaucuac     2880

gucagcgacu ucgccaagaa cgagcugccc aacaaggcca ucugcgcgaa caaggacgag     2940

aaggagugga ucgagaugac cgagaaguac aaguucaagu ucagccucua cccgaacgac     3000

cuggugaaga ucaagcggaa gaacgagaag gaguucuacg ccuacuacug cuccacccac     3060

cgcggcacgg ggaacaucaa ccugcuccug gugaacggcg aggcccggaa ggacggcguc     3120

gggaucaaga acaccgagau cuucgagaag uaccaggugg acgugcuggg caacaucagc     3180

aaggucaaca aggagaagcg cgagggggug aaguga                               3216


<210>  1528
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1528
auggacuacc gcaucggccu ggacaucggg accaacagca ucggcugguc caucaucgag       60

cucaccacgg ugcagagcaa gcuggacggg aaguaccggc ugaaggccgu cggccucauc      120

gacgccgggg ugcgcauguu cgaccgggcg gaggugccca agaccggcgc cucccuggcc      180

cugccgcgcc ggcucgcccg cagcucccgg cgccggcuga cccgccggag cgcccgcaag      240

aaggcgaucc ggaaccugcu cgucaaggag gacauccuga accagaucga gcuggaccac      300

cucuaccgca ccuccgagcu gguggacauc uggcagaucc ggguggaggg ccuggagcgc      360

cggcucgggc gccaggagug ggcccggcug cugauccacc ucggccagaa gcgcggguac      420

cggagcacgc gcaaggccga ggagggcgcc gacaccgagg ggcgcgcggu ccuguccagc      480

aucaaggaga accaggagac ccugaagcag uaccggaccg ugggcgagau gugggccacg      540

gacgacaagu ucaagggccg cgugcggaac uccaccaacg aguucaucuu caacgucagc      600

cgcgacaacc ucgaggagga gauccgguuc cuguuccagc agcagcgcga gcuggggucc      660

auguacgcca gcaagcuccu ggaggagucc uacauggagg uguggggcca ccagcugccc      720

uucgccagcg gggaguccau ccucaagaac guggggaccu gcagccugua cgagaaggag      780

aagcgggccg cgaaggccac cuacacguuc caguacuuca uggcccugga caagcucaac      840

aacguccgca uccugcccuc caugcggacc cugagcgagg aggagcgcgg ccucgcccug      900

aaggccaugu ucgagcggga caagcugccc aagaacccgc ccaaguucac cuacagccac      960

cuccgcaagg cgaucaagcu ggaggaggac gagaccuucc agacgaucuc cuacgacgug     1020

gagaagaccc uggccaccaa cgagagcgcc guguucguca accucgccgc cuacuaccag     1080

aucaagaagg ugugcgacga gcacaacgag aacuacgacu acguggacua caacaccauc     1140

gggcacgcgc ugacggucua caagaccgac gaggacauca acaaguaccu ggccuccaag     1200

aacaacaucg ccaagcgggu guacgccaac gacuucaucg acagcgugcu caagcacucc     1260

uacaccaccu ucggccaccu gagccugaag gcccucuccc gcaucauccc ccugauggag     1320

gagggggagg ucuucgcgaa ggccaaggag aaggccggcu ucaaggagga gagcuaccug     1380

gagaucaagu ccaagcuccu gacgaacauc cccgacgcca ucaagaaccc gguggugaug     1440

cggagccuga cccaggcgcg caaggucauc aacgccauca ucaagcggau gggggagcag     1500

cccgccucca uccagaucga gcuggcccgc gagcucagca aggacguguc cgagcggaag     1560

aagaucacca acggcuucgc cgagaaccgc agccgcaacc aguucgcgau caagacccug     1620

gaggagaacc acaucacgaa cauccggggc caggacaucg ucaaguucaa gcuguggcag     1680

gagcagggcg gccgcugcgc cuacucccuc aagcccauca agcuggagga gcugaucagc     1740

uuccucaaga aggagcggcg cuacgagcac aaccuggacg uggaccacau cauccccuac     1800

uccaagaccu ucgacgacag cuacaccaac aaggugcugg ucuucuccga cgagaaccgg     1860

aagaaggggg accagauccc ggccgaguac cucggcaucg ggaccaagcg cugggagaag     1920

uacaaggagu ucgugaucac gagcaaguuc cccgccagca agaagaagcg gcugcugaag     1980

gagcagcucg uggagcgcga ggagcuguuc augaaggagc ggcaccugaa cgacacccgc     2040

uacgccuccc gguacuucau gaacuuccuc cgcgagaacc ugcugcggaa ggagaccguc     2100

cuccacgaga cccagugggu gacggcggug gccggccgcg ucaccagcca cauccggaag     2160

cgccugggcu ucgggaagga ccgggaggag acccaccucc accacgcccu ggacgccauc     2220

aucguggccu gcaccgacaa guggaugauc gacugcguga ccaacuacua caagucccgc     2280

gagggcggga gcaucaagcg gaaggagauc cgcuuccccu cccccuggaa cggcuucugg     2340

cacgacgucc agacgaagcu ggcgcagcug ccgcagcccg aggugaucaa cgagagcauc     2400

uccaagggga accccgacgc cguggacuac augcucguca gccggaugcc ccgcaagucc     2460

aucaccggcg ccgcccacaa ggagaccauc agcaaguggg gcggguacga cgacaagggc     2520

aaggcgcugc uggugaagaa gguggcccuc uccgagcuga aguucaagaa cgacgacuuc     2580

ccgauguaca acaaggagac cgacccccug cucuacgagg ccaucaagaa ccgcuacguc     2640

gagagcgggc gggacgugaa gcgcgccuuc gccacgcccc uguacaagcc cuccaagagc     2700

ggcaagcgga acgaggugaa gcgcgucaag gugaucgcgg agaagaaggc cauggugcgg     2760

gaggucaacg gcgggaucgc cgagaacggc gggcuggugc gcuccgacau cuuccucaag     2820

gagaagaagu acgugauggu cccgguguac accuucgaca ccaucaagca gcagcugccc     2880

aacaaguacg uggucagcgg ccggggguac gagcaguggc ccgagcugac cgccgagcac     2940

guguuccagu ucuccgugua ccccuacgag cucguccacg ugggcacgaa caagggcgac     3000

aacagcuucu acuacuucgc cagccugaac aucuccagca acgugcugaa guucuccgag     3060

ugcaaccgcc cgaccaagga cuacaucgag aucgggcuca acagcaucau ccugcuggag     3120

aaguacgaga ucgacguccu cggccggauc accgggcagu ccaagaaggg cauccgccag     3180

agcuucaaga acaacaaguc ccagaugaag gaguga                               3216


<210>  1529
<211>  3219
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1529
augcgccugg gccucgacau cgggaccaac agcgugggcu gguggcugua caagaccgac       60

ggggaggccg ccuccgcgcg gaucgucgag gugaucgacg gcggggugcg caucuucagc      120

gacggccggg accccaaguc caagacgagc cuggccgucg accgccgggu ggcccgcgcc      180

gcccggcgcc ggcgcgaccg guaccuccgc cggcgcgcgu cccugaugaa gcugcucgcc      240

cggaccggcc ugaugccggc cgaccccgug gaggccaaga agcuggagcg ccucgacccc      300

uacgagcugc ggagcaccgg gcuggacgac gcgcuccccc ugacccacuu cggccgcgcc      360

cuguuccacc ucaaccagcg gcgcggguuc aaguccaacc ggaagacgga ccgcggcgac      420

aacgagaacg ggaagauccg cgaggccacc gcccggcugg accuggagau gaugaccagc      480

ggcgcccgcu ccuacggcga guuccuccac augcggcgcc agcgggcgag cgacccgcgc      540

cacguccccu ccgugcggac ccgccugagc guggccgccc gcgggggccc cgacgccaag      600

gccgaggcgg gguacgacuu cuaccccgac cggcaccacc uggaggagga guuccacgcc      660

cucugggccg cccaggccac gcaccaccag gcgcugaccg acgaccugcg ggaccucguc      720

uucgagaaga ucuucuacca gcgcccgcug aaggagccca agaucggccu gugccucuuc      780

accgccgagg agcggaucgc caaggcccac ccccugaccc agcgccgggu gcuguucgag      840

acgaucaacc agcuccgcgu gaccgccgac ggccggucca cccgcccccu gacccuggag      900

gagcgggaca cgcucgucca cgcgcuggac aacaagaagc cgaccaagag caugaccucc      960

augaacauga cccugaaggc ccucgccaag cugcugaagc uccgcgacgg ggagcgcuuc     1020

acgcuggaga ccggcgugcg ggagaacauc agcugcgacc ccgugcgcgc cugcauggcg     1080

caccccgccc gguucgggcc ccaguggucc agccuggacu gggaggccca gugggacguc     1140

aucucccgcg ugcgccaggu gcagagcgag ggcgaguuca ucgagcucac cgacuggcug     1200

auccaggggc acggccugga cgccgaccac gccaaggcgg ucggcaacgc cccgcucccc     1260

gagggguacg cccggcuggg cuucagcgcc accgagcgga uccuggacca gcucaaggcc     1320

gaggugugca cguacuccga cgcgguggcc aagugcgggu ggcaccacag cgaccagcgc     1380

accggcgccu gccuggaggu ccugcccuac uacggggagg ugcuggaccg gcacgugauc     1440

cccggcaccu acgacgccac cgacgacgag aucacgcgcu acggccggau caccaacccg     1500

accguccaca ucgggcucaa ccagcugcgc cggcugguga acaagaucau cgagguccac     1560

ggcaagcccg accagaucgu gguggagcuc gcccgcgagc ugaagcaguc cgagccccag     1620

aagaaggacg cgaugaagcg gcugcgcaac accacggccg acgccauccg gcgcagcgag     1680

cagcucgagc agcuggggca gcccaacacc ggccacaacc ggaugcugcu ccgccugugg     1740

gaggaccugg ggccggccau cggcccccgg ugcugccccu acaccggcaa gaccaucucc     1800

gucacgaugg uguucgacgg gagcugcgac guggaccaca uccuccccuu cucccgcacc     1860

cuggacgaca gcuucgcgaa ccgcacccug ugccucaagg aggccaaccg ggagaagucc     1920

gaccgcaccc cgugggaggc cuggggcggg accgaccagu gggacgacau cgccgccaac     1980

cugaagaacc ugcccgagaa gaagcggaag cgcuucggcc ccgacgcgau ggagugguuc     2040

gagggcgaga acgacuuccu cgaccgggcc cuggucgaca cgcaguaccu ggccaagauc     2100

agccgcaugu accucgacac ccuguucacc aagggcgggc acgugugggu ggucccgggg     2160

cggaugaccg agaugcugcg ccggcacugg ggccucaacg cccugcugaa cgacaaggac     2220

cgcggggccg ugaaggcgaa gaaccggacg gaccaccgcc accacgccau cgacgccgcc     2280

guggucgccg cgaccgacua ccggcucaug cagaagaucu cccgcgccgc cggccagggc     2340

gagaacgccg ggcagagcgc ggagcugauc gcccgggaca ccgacgcccc cugggagcac     2400

uuccgcgccg acaucgccuc ccagcuggag aagaucaucg ugagccaccg ggcggaccac     2460

ggccgcaucg acguggaggg gcggaagacc ggccgcgacu ccacggccgg gcgccuccac     2520

aacgacaccg ccuacggcgu caucgacgac cugaccgugg ccagccggac gccccugaug     2580

ucccucaagc ccagcgacau cgccaucacc gagcgcggca agaacauccg ggacccgcag     2640

cugcagaacg cgcuguacgu ggccaccaag gggaaggagg gcaagauguu cgagcaggcc     2700

cucgucgacu ucgccuccaa ggaggggccc uaccagggca uccgccgggu gcgccuggug     2760

gagacccugc agaaggccgc gcgggucgag aucgggaaga ccgagagcgg ccagccccuc     2820

aaggccuaca agggcgacag caaccacugc uacgagcugu ggaagcugcc cgacgggaag     2880

aucgugcccc agguggucac gaccuacgag gcccacgccg gcuccgcgcc ccgcccccac     2940

cccgccgcca agcggauccu ccgcguguuc aagaaggaca uggugaagcu gaccaaggac     3000

ggggaggaga ucgucggcua cgugcagagc augcacauca agaacgggcu guucguggcc     3060

cagcaccucg aggccaacgc ggacgcccgg aaccgcgaca agaccgaccc guucaaguuc     3120

cugcagaugg gcgccggcac gcuggucaag accgggcucc ggcgcgugcu gguggacgag     3180

aucggccggc ugcgcgaccc cgggccgccc aagcucuga                            3219


<210>  1530
<211>  3225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1530
augcagacca agaaccugcc cuacacccuc ggccuggaca ucgggauggc cagcgugggc       60

gccgcgcugc uccugccgga ccaggagcgc auccuggccc uccacguccg gacguucgac      120

aaggccgaga ccgccaagga gggggagucc cugaacaaga uccgccggga cagccgccug      180

acccggcgcc ggauccgccg gcgcgcccac cggcucgugc gccuggcgcg gcugaugaag      240

cgcgugggcc ucaucgccga cgccaacccc gaggccuucg cgcugcccgg ggucuccccc      300

uggcaccucc gggccgacgg ccucgaccgc cugcuggagc cgaaggagug ggccggcgug      360

cucuaccacc uggugaagca ccggggguuc cagagcaacc gcaaguccga ggccaaggcc      420

gacgagaagg cgggccagau gcugagcggg gucggccgga accaggcccu ccugaaggag      480

gccggguacc gcaccguggg cgagcuggcc gcccggcacg aggacuucaa ggaggcgaag      540

cgcaacaagg gcggguccua cagccacacg uucgcccgcg ccgaccucaa cgccgagcug      600

aaccugcugu ucgagcggca gcgcgcccug ggcucccggc uggcgagcgc cgacuuccag      660

gagaacgugc gcgaccuccu gauggcccgg cgccccaccc uguccggggc caaccuccug      720

aagauggucg gcaagugcac cuucgagaag uccgaggacc gggcccccaa ggcgggcuac      780

cgcgccgagc gguucgugug gcuggggaag cucaacaacc ugaagaucgu gggcagcggg      840

gacgcccgcc cccugaccga cgccgagcac cggaucguca ucgaccuccc guucacgcag      900

gcgaagcuga ccuucaagca gguccgcaag gcccuggagc ucgagaccca ccagcgguuc      960

aaccugcuga gcuaccgccc cgaccccaag ggcaaggaca aggaccccga ggaguccacc     1020

uucuucgagg ccaagagcuu ccacacgcuc cggaaggccu acgagggggc cggccugaag     1080

aaggaguggc agcgcgacgc gcuggacccg gaccggcucg acgcccuggc cuacgcccug     1140

accugcuaca aggacgacac cgagucccgc caguggcugg uggcccaggg caucgggacc     1200

cccaucaucg aggcggugcu cgccgagagc uucgacaagu ucauccaccu gucccagaag     1260

gcccugaagg ccauccuccc ccacauggag gccggccagc gguacgacga cgcggccaag     1320

agcgccgggu acgaccacag ccagcccgac gccggcacgg ggaagcgcgc gacccugccg     1380

ccgcccgaca aggacaccau ccgcaacccc gugguguacc gggcccugaa ccaggcccgc     1440

aagcucguca acgccaucgu ggccgaguac ggcccgcccg cggccgugca caucgagcug     1500

gcccgggacc uguccaagcc caucgacgag cgccgggaca ucgagaaggg ccagaaggag     1560

uuccaggccc ucaaggagaa gcuggccgcg gccuucgagg agaccuucca ccgcaagccc     1620

agcgggcugg accuccugaa guaccggcug uuccgcgagc agcucgacca gugcccguac     1680

ugccagaagg cccuggucgu ggaccggcug uucgagcccg gcuacgccca gaucgaccac     1740

gcccuccccu acucccgcag cuucgacgac gggaugaaca acaagguggu cgugcacguc     1800

gcgugcaacc aggacaaggg caaccggacg cccuacgagu uccuggacgg ggccggcgac     1860

uccgagcgcu ggcagcgguu cguggccugg gugcagggca acaaggccau ccgccaggcg     1920

aagcggaacc gccugcuccg ggucaacuuc gggaccgacg aggccaagga guucagcaag     1980

cgcaaccugu ccgacacccg guacaucugc cgcgaguuca agcggaccau cgagacgcag     2040

uucgccuggc acagcgacgc cgagggcaac gagcgcugcg uggugcuguc cgggcagcuc     2100

accagccugc ugcgcgcccg guggggccuc aucaaggucc gcgaggacgg cgaccugcac     2160

cacgcgcugg acguggccgu gaucgccgcc gccucccgga gccucgucaa gcgcauggcg     2220

gacuacuccc ggcgcaacga gcuggagaug gugcggaacc gcuacgugga cccggccacc     2280

ggggaggucg uggacguggc cgcccugcgg cagaucgagg accacuuccc caagcccugg     2340

ccccucuucc gcgaggagcu gcuggcccgg cucgcgcccg agcccaagcu gcagcugacc     2400

ugcgucagcu ccuacgacga gggccuccgc gagagcaucc ggcccauccg cgugucccgg     2460

gccccgacgc gccgggggcu gggcgccgcc caccaggaga ccauccgcag ccugcgggac     2520

gggucccaga gcgccaucaa gacgccccuc cgcgaccuga accugaagga ccucgagaac     2580

aucgugggcu ucgagcgcga caccgcgcug aucgacgcca uccggacccg ccuggaggcc     2640

uucggcgggg agggcaagaa ggccuucgac gacaagcagc cgccccuccg gaagcccagc     2700

aaggaccccg acaaggcgcc caucauccgc uccgucaagc ugcuggugac ccagaagucc     2760

gggaucccca uccggggcgg gaucgccaac aacggcgcca ugcuccgcag cgacaucuuc     2820

accaaggccg gcaaguucua cgccgugccg aucuacgucg cggacgccgu gcgcggggag     2880

cugccccacc gggccguggc ccaggccaag cccgagcugg aguggcccau gauggacgag     2940

agcuaccagu uccucuucuc ccugcacccg aacgacugga ugaccauccg gcugaagaac     3000

gagguccgcg agggcuacua cgcggggcuc gaccgggcca cgggcaacau cagccugugg     3060

cugcacgacc gcaaccagaa cguggggcag aagggccucc ugaccggcaa cgggaucaag     3120

accgcccugg ccguggagaa guaccacguc gaccuccugg gcaagcugca ccgggugcac     3180

aaggaggagc gccagcccau ccacaucaag cccgggaagc gguga                     3225


<210>  1531
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1531
augaaguaca gcaucggccu ggacaucggg aucgccuccg ugggcugggc cgucaucaac       60

gaggacaucg agcgcaucga ggaccucggg gugcggaucu ucgacguggc ggagcagccc      120

aagaccggcg ccagccuguc caccccgcgc cggcuggccc gcagcacgcg gaagcgccuc      180

cggcgccggc gccaccgggu cgagcgcgug cggaaguuca ugauccagaa ggggcugcug      240

acccaggugc agauggagca gcucuacgac uggaaggacg gcgaccugga caucuggcug      300

guccgcguga acgcccucga gcggaagcug uccgaccgcg aguucgcccg gauccuggug      360

cacuucgcga agaaccgcgg cuuccagagc aaccggaagu ccgagcucca ggaggaggac      420

aacggggccg uccugagcgc cgugcaggag aacaagcgcc ugauggagga gaagggcuac      480

cagaccaucu ccgagaugcu ccuguacgac gagaaguucg aggggaccaa gcggaacaag      540

ggcggggagu acacgcacgu ggucgcccgc agcgaccuga agcgcgagau ccagcucauc      600

uucgagaagc agcgggcgua cggccacgcc uucgccacca uggagaacga ggacaaguuc      660

cugcagaucu gguccagcca gcgccccuuc gccaccaagg acgacaucgc caagaagauc      720

ggcaacugca ccuucgagcc caaggagaag cgggcgccca aguccacgua caccuucgag      780

cgcuuccggg cccuggacaa gcucaaccgc cugcggaucc ugagcgggac cuccccggcc      840

cgcagccuca cccaggagga gcgggaccug auccugaacu cccucuucag caagaaggag      900

gugaaguaca aggagcugcg caagcugcuc aagcugaacg acgagcagcg guucaacgag      960

aucuucuacg accccaacga gaagaucgag aagaacgaga accgcacguu ccugucccuc     1020

gagagccagu acaagauccg gaagauccug cagaagaucg agggcaagag ccugaacgac     1080

accuaccgcc ccguggaccu cgacaccauc gccuacgccc ugaccgucuu caaggacgac     1140

caggacaucc gggacuaccu gcagaaccag uacguggcgu ccaacgggaa gaaggagcuc     1200

aaccuggcca accgcaagua caacgacgag cugaucgagg agcuccugca ccugagcuuc     1260

acgaaguucg gccaccucuc ccugaaggcc cugcggaaca uccuccccuu cauggagcag     1320

ggcaucccgu accaccaggc cugcgagaag gccggguacc aguucaacaa gaaggugaac     1380

cgcgagaagc agaagcugcu gccgcccguc cccgcgaagg acaucgugaa ccccgugguc     1440

auccggagcc ugucccaggu gcgcaaggug aucaacggca ucaucaagcg cuacgggagc     1500

cccuccagca ucuacaucga gcucgcccgg gagaugggcc gcggguacaa ggagcggaag     1560

gaccuggaga agcaguacaa caagaaccgc cagaugaacg agggcgccaa gaagcacauc     1620

cuggagcucc ggcccgaguu cggcgacccc cgcgggcacg acauccugaa guacaagcug     1680

uggcaggagc aggacugccg gugcgccuac uccuucaaga gcaucccgau caagcagcug     1740

uucgagaucg gcuacgccga ggucgaccac aucauccccu acucccgcag cuucgacgac     1800

uccaacaaca acaaggugcu cguccuggcg gccgagaacc agaacaagca gaaccggacc     1860

cccuacgagu gguucgggca cgacgagacc aaguggcagg aguucaccag ccucgugacg     1920

accaugaagg uguccaagaa gaagaagucc cugcugcuca agaagaacuu cgacgaggag     1980

caggccgagg aguuccgcag ccggcaccug aacgacaccc gcuacaucac ccgguacauc     2040

aagacguuca ucgaggacaa ccugcaguuc cgcaucgagg agggcaagaa gaccuacguc     2100

cacaccguga acggggccua caccagccuc augcggaagc gcuggggcuu caacaagaac     2160

cggcaggaga acgcgcugca ccacgcccug gacgccguga ucgucgccgu gucccucccc     2220

uucaagcaca aggugcgcgc cuacuucaag cggcgcgagg aguacgcgca caaccagcug     2280

aagcgggagg gcgaguucuu cccggagccc uggcccgggu ucauccacga gcuggagauc     2340

cgcaugaugc aggacagcga gaacaucaag cucgccaugg agagccugaa ccugaagacg     2400

uacgacgagu ccuucaucaa ggagcucaag cccaucuucg ucagccgcau gccgaagcgg     2460

gccaugaagg gccagaucca cgaggagacc cugaagcgcg ugcgggggcg caccgacgac     2520

ggcuacaucc ggaccgugaa gaaggucaag cuggaccaga uccccuucga caagaacggg     2580

gacuucccca uguucggcaa ggagacggac augaagaccu acgaggccau caagucccgc     2640

uaccucgaga gcggcaagga caagaagaag gccuucaaca cgccccugua caagccggcg     2700

aagaaccccg agaacgcccc caucauccgg uccaucaaga ucgaggagaa ggccaaccgc     2760

guggugaugc uggacgagaa gaccgccgcc gacaacgcga gcaucguccg gaccgaggug     2820

uuccgccaca aggagaccgg gaacuacuuc cucaccccgg uguacgucgc cgacgugcug     2880

gccaagaagg ugcccgaccg gcugaucacc accaagaagc ccuacuccga guggcgcaag     2940

aucacggacg aguacgaguu ccucuucagc cuguacccca acgaccugau ccgggucaag     3000

augccgaagg agaaggagac caagacccac accggcgaga agcacgugug gcaggagaac     3060

uucauguacu ucaagggggu gcacuccggc agcgccggcg uccgcaugau cgaccacaug     3120

aacuccuucg aggacgagau cggggugcgg aagcucgugg ucuucgagaa guaccaggug     3180

gacccccugg gccgcaugaa caaggugcac ggggagaagc ggcacggcau cuga           3234


<210>  1532
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1532
augaaguaca gcaucggccu ggacaucggg aucgccuccg ugggcugggc cgucaucaac       60

gaggacaucg agcgcaucga ggaccucggg gugcggaucu ucgacguggc ggagcagccc      120

aagaccggcg ccagccuguc caccccgcgc cggcuggccc gcagcacgcg gaagcgccuc      180

cggcgccggc gccaccgggu cgagcgcgug cggaaguuca ugauccagaa ggggcugcug      240

acccaggugc agauggagca gcucuacgac uggaaggacg gcgaccugga caucuggcug      300

guccgcguga acgcccucga gcggaagcug uccgaccgcg aguucgcccg gauccuggug      360

cacuucgcga agaaccgcgg cuuccagagc aaccggaagu ccgagcucca ggaggaggac      420

aacggggccg uccugagcgc cgugcaggag aacaagcgcc ugauggagga gaagggcuac      480

cagaccaucu ccgagaugcu ccuguacgac gagaaguucg aggggaccaa gcggaacaag      540

ggcggggagu acacgcacgu ggucgcccgc agcgaccuga agcgcgagau ccagcucauc      600

uucgagaagc agcgggcgua cggccacgcc uucgccacca uggagaacga ggacaaguuc      660

cugcagaucu gguccagcca gcgccccuuc gccaccaagg acgacaucgc caagaagauc      720

ggcaacugca ccuucgagcc caaggagaag cgggcgccca aguccacgua caccuucgag      780

cgcuuccggg cccuggacaa gcucaaccgc cugcggaucc ugagcgggac cuccccggcc      840

cgcagccuca cccaggagga gcgggaccug auccugaacu cccucuucag caagaaggag      900

gugaaguaca aggagcugcg caagcugcuc aagcugaacg acgagcagcg guucaacgag      960

aucuucuacg accccaacga gaagaucgag aagaacgaga accgcacguu ccugucccuc     1020

gagagccagu acaagauccg gaagauccug cagaagaucg agggcaagag ccugaacgac     1080

accuaccgcc ccguggaccu cgacaccauc gccuacgccc ugaccgucuu caaggacgac     1140

caggacaucc gggacuaccu gcagaaccag uacguggcgu ccaacgggaa gaaggagcuc     1200

aaccuggcca accgcaagua caacgacgag cugaucgagg agcuccugca ccugagcuuc     1260

acgaaguucg gccaccucuc ccugaaggcc cugcggaaca uccuccccuu cauggagcag     1320

ggcaucccgu accaccaggc cugcgagaag gccggguacc aguucaacaa gaaggugaac     1380

cgcgagaagc agaagcugcu gccgcccguc cccgcgaagg acaucgugaa ccccgugguc     1440

auccggagcc ugucccaggu gcgcaaggug aucaacggca ucaucaagcg cuacgggagc     1500

cccuccagca ucuacaucga gcucgcccgg gagaugggcc gcggguacaa ggagcggaag     1560

gaccuggaga agcaguacaa caagaaccgc cagaugaacg agggcgccaa gaagcacauc     1620

cuggagcucc ggcccgaguu cggcgacccc cgcgggcacg acauccugaa guacaagcug     1680

uggcaggagc aggacugccg gugcgccuac uccuucaaga gcaucccgau caagcagcug     1740

uucgagaucg gcuacgccga ggucgaccac aucauccccu acucccgcag cuucgacgac     1800

uccaacaaca acaaggugcu cguccuggcg gccgagaacc agaacaagca gaaccggacc     1860

cccuacgagu gguucgggca cgacgagacc aaguggcagg aguucaccag ccucgugacg     1920

accaugaagg uguccaagaa gaagaagucc cugcugcuca agaagaacuu cgacgaggag     1980

caggccgagg aguuccgcag ccggcaccug aacgacaccc gcuacaucac ccgguacauc     2040

aagacguuca ucgaggacaa ccugcaguuc cgcauggagg agggcaagaa gcaguacguc     2100

cacaccguga acggggccua caccagccuc augcggaagc gcuggggcuu caacaagaac     2160

cggcaggaga acgaccugca ccacgcgcug gacgccguga ucgucgccgu gucccucccc     2220

uucaagcaca aggugagcgc cuacuucaag cgccgggagg aguacgccca caaccagcug     2280

aagcgcgagg gcgaguucuu cccggagccc uggcccgggu ucauccacga gcuggagauc     2340

cggaugaugc aggacagcga gaacaucaag cucgcgaugg agucccugaa ccugaagacc     2400

uacgacgaga gcuucaucaa ggagcucaag cccaucuucg ucucccgcau gccgaagcgc     2460

gccaugcggg gccagaucca cgaggagacg cugaagcgcg ugcgggggcg caccgacgac     2520

ggcuacaucc ggaccgugaa gaaggucaag cuggaccaga uccccuucga caagaacggg     2580

gacuucccca uguucggcaa ggagaccgac augaagacgu acgaggccau caagagccgc     2640

uaccucgagu ccggcaagga caagaagaag gccuucaaca cgccccugua caagccggcc     2700

aagaaccccg agaacgcgcc caucauccgg agcaucaaga ucgaggagaa ggccaaccgc     2760

guggugaugc uggacgagaa gaccgccgcc gacaacgccu ccaucguccg gaccgaggug     2820

uuccgccaca aggagaccgg gaacuacuuc cucaccccgg uguacgucgc ggacgugcug     2880

gccaagaagg ugcccgaccg gcugaucacc accaagaagc ccuacagcga guggcgcaag     2940

aucaccgacg aguacgaguu ccucuucucc cuguacccca acgaccugau ccgggucaag     3000

augccgaagg agaagggcac gaagacccac accggggaga agcacgugug gcaggagaac     3060

uucauguacu ucaagggcgu gcacagcggc uccgccgggg uccgcaugau cgaccacaug     3120

aacagcuucg aggacgagau cggcgugcgg aagcucgugg ucuucgagaa guaccaggug     3180

gacccccugg ggcgcaugaa caaggugcac ggcgagaagc ggcacgggau cuga           3234


<210>  1533
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1533
augcagcaga agccccagcg cuacauccug ggccucgacc uggggaucgc cagcgugggc       60

ugggccugcg ucugggcgga cgagaacgag cacccgaccg ggcugcucga cugcggcgug      120

cggaccuucg agcgcgccga ggugcccaag acgggggacu cccuggcccu ggcccggcgc      180

caggcccgga gcguccgccg gcucauccgc cggcgcgcgc accggcugcu gcgccuccgg      240

cgccugcuga agcgggaggg cgugcuccug uccgccgacu ucaacgagaa cggccuggug      300

cgcgggcucc ccaucgacgc cugggcccug cgggucgcgg gccuggaccg caagcucagc      360

aacaaggagu gggccgccgu gcugcugcac cucgugaagc accgggggua ccugucccag      420

cgcaagaacg agacccagag caaggacaag gagcugggcc ggcuccuggc cggggucaac      480

ggcaaccgcg aggcccugca gaaccgcucc gacgaguacc ggaccgcggc cgagcucgcc      540

gugaagaagu ucgccgccga gaacaacggc agccugcgca acaagggcgg ggacuacucc      600

cacaccuucg accggaagga ccugcagcgc gagcuccacc uccuguucga gcggcagcgc      660

gcgcuggaca accccuucac gagcuccgag auggaggccg ccguggacga gcugcugaug      720

aagcagcgga gcgcccucca gggggaggcc guccugaaga ugcugggccg cugcaccuuc      780

gagccguccg aguacaaggc ggccaagaac accuacagcg ccgagcgguu cguguggcuc      840

accaagcuga acaaccugcg cauccaguac aacggcgagg agcgggcccu cuccgaggcc      900

gagcgccggc ugcugcucga cgagcccuac cagaaggcga agcugacgua caagcagguc      960

cgcaccaucc uggagcuccc cgagagcgcc guguucaagg ggcugcggua cggccacggg     1020

gaggacggcc ugaaggccga guccgggacc cugauggaga ugaaggccua ccacgcggug     1080

cgcaaggccc ucaaggacgc cggccuggag ggcgaguggg agaaccggau ccgcaacccc     1140

gagcugcucg acgccaucgg gaccgccuuc agccuguaca agacggacga ggacauccgg     1200

gcgaaccugg ccgccuuccg ccucuccgag aacgugcugg aggcccugcu cgccggccug     1260

aacuucgaca aguucgucca ccugagccuc aaggcgcugg ccaagauccu gccgcucaug     1320

gagcagggga ugcgcuacga cgaggccugc aaggaggccu acggcgacca cuacggggcc     1380

aaggagcagg aggaccaccg gcugcugccg cccuucgugg cggacgacau ccgcaacccc     1440

guggucuucc ggacccucac ccaggcccgc aagaucguga acgagaucgu gcggcgcuac     1500

ggcagccccc ugcgggucca caucgagacc ggccgcgagg uggggaaguc cuucagcgac     1560

cggaaggaga ucgagaagcg ccaggaggag aaccggaagg agcgcgagcg ggccgccgcg     1620

cacuucaagg agcuguuccc caacuucgcc ggcgagccca aggcccagga cauccucaag     1680

cugcgccugu acgagcagca gcacgggaag ugccucuacu ccggcaagga gaucgaccug     1740

cggcgccugc ccgagaaggg guacgucgag aucgaccacg cccucccguu cagccggacg     1800

ugggacgacu ccuucaacaa caaggugcug gugcugggca aggagaacca ggacaagcgc     1860

aaccggaccc ccuacgagua ccucgacggc aaggggaaca gcggcgccug gcgcgcguuc     1920

gccgagcggg ucuccgggug ccgcuucccc uacgccaaga agcagcgccu gcugacccag     1980

aagcucgacg agaagaccga gcagggcuuc cuggagcgga accugaacga cacgcgcuac     2040

guggcccggu uccucugcca guucaucgag gagaagaugc accuggaggg cgccgggaag     2100

aagcgcgugu ucgcgagcaa cggccagauc accgcccugc uccggggguu cuggggccug     2160

aagaaggucc gcgaggagaa cgaccggcac cacgcccugg acgccguggu ggucgccugc     2220

uccagcgugu ccaugcagca gaagaucacc cgccacuucc gguaccgcga ggagcucgag     2280

cacaucgacc gggagaccgg ggaggugauc cacggcuucc cccagccgug ggaguucuuc     2340

cgccaggagg ucaugacgcg gguguucagc gacgaccccg uguccgagcu ggcggagaag     2400

cuggacagcc gccccgaggc cguccacgag uucgugacgc cccucuucgu gucccgggcc     2460

ccgcaccgca aggucaccgg ccaggggcac cuggagacca uccggagcgc caagcgccug     2520

gacgagaaga ucuccguggu gaagcucccc cugacccagc ugaagaccaa ggacaucgag     2580

cggaucgucg gcuaccccaa ccgcgagccc gcgcucuacg acgcccuggc cgcccgccug     2640

gccgcguuca aggacgaccc ggccaaggcc uucagcgagc ccuucuacaa gaccgggaag     2700

gacggcaagu gcggggugcu cgugaaggcc guccgggugg agaacgugca gaaguccggc     2760

cugcuggucc gcaaggacga gagcggcaag augcaggggg uggccgacaa cgcgaccaug     2820

gugcgggucg acguguucgg caagaacggg aagaacuacc ucgugcccgu cuacgccugg     2880

caggucgaga agggcgagcu gccgaaccgc gccguggugg ccuacgccga cgaggaggac     2940

ugggacgaga uggacgacag cuucgaguuc aaguucuccc uguaccccaa cgaccucgug     3000

gaggugguca cgaagaagga caaguucuuc ggguacuaca gcggcuucaa ccgggcgacc     3060

ggcgccauca acaucaagga gcacgaccug uccaagagca aggugaagaa cgggguguac     3120

gagggcaucg gggucaagac cgcccagucc uuccagaagu accaggugga cacccugggc     3180

aagacgaucc gccucugcaa gcccgagaag cggauggggu ucaagaagaa guga           3234


<210>  1534
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1534
augcgcuacg ugcugggccu cgacaucggg aucgccagcu gcggcugggc cgucaucaac       60

caggagaagg agcggaucga ggaccugggg gugcgcaucu ucgacaaggc ggagaacccc      120

aaggacggca agucccuggc cgccccgcgg cgcgacgccc ggagcacccg ccggacccuc      180

cgccggaaga agcaccgcau gcagcggauc aaggugcugc uggucaagca cgggcuccug      240

uccaagacgg agcuggacca ccucuacgag agcgccaccg agguggacgu guggaaccug      300

cgccuggacg cgcucgagcg gaagcuguac cccaaggagu ucgcccgcau ccugauccac      360

cucgccaagc ggcgcggcuu caaguccaac cggaaggaga ccacccugag cgagaacggc      420

cagauccugg aguccaucag cgagaaccag cagaucaugg agaagcagaa cuaccgcacg      480

gucggggaga ugauccucaa ggacaagaag uucgagaacc acaagcggaa caagaacggc      540

accuacgugg ggaccgugac ccgccagcag cugcaggacg agauccagau gaucuuccac      600

gcccagcggc uguacaagaa cgacuacgcg acggaggagu ucgaguccag cuaccucgag      660

aucugggccu cccagcgccc cuacgccagc aaggaccaga ucgagaagau gaucggcuac      720

ugcacccugg agcccaagga gaagcgcgcc ccgaaggccu ccuggagcuu ccaguacuuc      780

gucgcgcugc agaccaucaa caaccuccgg cugaucucca aggaccgcau cgaggagcug      840

agcuucgagg agaagaagca gaucaugaac cucgcccugg agaaguccau cgugaaguac      900

aucgacaucc ggaagcugcu cagcgugccc aacgaguucc aguucaacuc ccugagcuac      960

agcgccgaca ccgucgacac ggccguggag ucccgcaagu gcaucgagcu gaaggaguac     1020

cacagcauca acaaggugua caagcagauc uacggggaga acgcccucaa ccugcugccc     1080

aaguacuacg acaccaucgc guacggccuc accaucuuca aggacgacaa ggacauccug     1140

gaguaccugc agaacaagua cgucaacgcc aagggcaagc ccaucuccaa cuucgccaac     1200

aagaccuacg acgacacguu cauccaggcc cuccugaacc ugaacuucac caagaugggg     1260

caccucagcu ucaaggcccu gaagaacauc aucccguucc uggaggaggg ccucuccuac     1320

gacaaggcgu gcgagaaggc cggguacaac uucaagggca ccagcuacac cgagcagacg     1380

aaguaccugc ccgugauccc ccagaacacc aaccccgugg uccaccgggc ccugucccag     1440

acccgcaagg ugaucaacgc caucaucaag aaguacggca gcccgaacgc cauccacauc     1500

gagaccgcgc gggagcucuc caagacguuc caggagcgca aggagaucga cagcauguac     1560

caggacaacu ccaagaagaa cgagcacgcc auccacaagc ugaaggagcu ggggaacauc     1620

aaccccagcg gcaucaacau cgugaaguuc aagcugugga acgagcagga cgggcggugc     1680

auguacuccg gcaaguacau cgagccccac cgccucuucg aggaggggua caccgagguc     1740

gaccacaucc ugcccuacag ccggucccug gacgacagcu acaacaacaa gacccucacc     1800

cugggcacgg agaaccagcg caagggcaac aagaucccgu acgaguacau ggggaacacc     1860

uccaucugga acgacuucga gacccgggug cagagcaaca agaagaucaa caagaagaag     1920

cagcagaagc ugcuccugca gcacuucucc uacgcccgcg agcaggaguu caucgagcgg     1980

aaccugaacg acacccgcua cgccacgauc uaccucagca gccugaucca gcagcaccug     2040

aucuucuccg agagcucccg gaagaagaag gugcacaccg ucagcggcau caucaccucc     2100

caccuccgca gccggugggg guucaacaag gaccgcaagg agggccacau ccaccacgcg     2160

cuggacgccg ugaucgucgc cgugaccucc gaccacauga uccagcgcgu gacgaaguac     2220

uacaagcuga aggagcucaa ccggaaccug caggccaagc gcaugcccuu ccccgagccc     2280

ugggaggggu uccggcugga gcucgaggcc cgcaucagcc cgaacaccca gcaguaccug     2340

aagggccugc gguucaagaa cuacgcggac gucaaccucu ccgagaucaa gagcaucuuc     2400

gugucccgca ugcccaagca cagcaucacc ggcgagcugc accaggagac cauccggaag     2460

uucaucgggu acaacgagaa gggcaaggug cugaccgcca ucaagacgaa gcucgaggac     2520

auccccuucg acgccaacgg ggacuucccc auguacggca aggaguccga ccuguacacc     2580

uacaacgcca ucaaggagcg cuaccugagc cacaagaagg acaagcggaa guccuuccag     2640

gagccgcucu acaagcccac caagagcggg ggcaucggcc cccugaucaa guccaucaag     2700

aucauggaca cccgcacgau cgucaacccc gugaaccagg ggaagggcgu ggucuacaac     2760

agcaagaucg cccggaccga cguguucaag aaggaggaga aguacuaccu gaucccgauc     2820

uacaccaucg accucaugaa gaacauccug ccccagaagg cgaucaccgc cgggaagggc     2880

uacgaggacu ggacgaccau cgaccccagc uucaccuucc uguucucccu cuucccgaac     2940

gaccugaucc agaucgugcc cagcaagaac aagaccauca aggcccgcac gaccgucucc     3000

aagaaggagg ugcugcuccc cagccugacc ggcuacuuca agggggugca cuccggcacc     3060

gccgggauca cggucgagac ccacgacggc agcgugaucg ccaacguggg guccaagcag     3120

cugcuccuga ucgagaagua ccaggucgac gugcugggca acuacaccaa gaucaaggag     3180

gagaagcgga ucggccucac cacguacaac gacaacaaga agaucgcggc cugcuga        3237


<210>  1535
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1535
augaagcagc agccccagcg cuacauccug ggccucgacc uggggaucgc cagcgugggc       60

ugggcccuga uccucgucga cgaggagggg aacccgaucg gccugcugga cugcggggug      120

cggaccuucg agcgcgcgga ggugcccaag accggcgacu cccucgcccu ggcccggcgc      180

caggcccgga gcguccgccg gcugauccgc cggcgcgccc accggcuccu gcgccugcgg      240

cgccuccuga agcgggaggg cgugcugucc gcggccgacu ucaacagcga cgggcucccc      300

aaccugccca ucgacacgug gcagcugcgc guggccggcc ucgagcggaa gcugaccaac      360

caggaguggg ccgcgguccu gcuccaccug gugaagcacc gcggguaccu gucccagcgc      420

aagagcgaga cccagaccga gaacaaggag cucggcugcc ugcuggccuc cguggccaag      480

aacagcaagc uccuguccga gaacgccaac caguaccgga cgccggccga gcuggcgguc      540

cggcuguucg ccgagcagag cgggcaccuc cgcaacaagg gcggcgacua cacccacacc      600

uucaaccgcc uggaccucca gaaggagcug caccuccugu ucgagcagca gcggaccuuc      660

gggaaccccu ucgccuccgc cgagcugcag agcaaggugg acggccugcu caugaugcag      720

cgcuccgccc ugcaggggga ggcggugcug aagaugcucg gcaagugcac guucgagccc      780

agcgaguaca aggccgccaa gcacaccuac uccgccgagc gguucaucug gcugaccaag      840

cugaacaacc uccgcauccu ggagaacggc accgagcggg cccugaacga ggcggagcgc      900

aagcuccugc uggacgagcc cuacaagaag gccaagcuga cguacagcca ggcccggaag      960

cuccuggaca ugucccacga cgccaccuuc aaggggcugc gcuacggcaa ggacagcgag     1020

gggcucaacg ccgagaacgg cgcgcugaug gagaugaagg ccuaccacaa gauccggacc     1080

guccuggaca aggccgggcu caagggcgag ugggagaacc gcauccggaa cccggagauc     1140

cuggacaaga ucggcaccgc cuucucccug uacaagacgg acgaggacau ccaggcggcc     1200

cucgccgacc ugcgccugcc cgagaacgug cucaacgccc ugcuggccgg gcucaacuuc     1260

gacaaguuca uccagcugag ccugaaggcg cucagcaaca ucaugccccu gauggagcgg     1320

ggcauccgcu acgacgaggc cugcaaggag guguacgggg accaccgggc ccuggccgag     1380

uccaagaacc agaugcuccu gcccccgauc cccgccaacg agauccgcaa ccccgucgug     1440

cugcggaccc ucacccagac ccgcaaggug aucaacgcga ucauccggcg cuacggcagc     1500

ccccagcgcg uccacaucga gacggcccgg gagcugggga aguccuucaa ggaccgcaag     1560

gagaucgaga agcggcagga ggagaaccgc aaggagcggg agcgcgccgc cgagcacuuc     1620

aaggagcugu ucccgcacuu cgccggcgag cccaagggcg gggacauccu caagcugcgg     1680

cuguacgagc agcagcaggg caagugccuc uacagcucca uccccaucga ccuggggcgc     1740

cugaacgaga agggcuacgu ggagaucgac cacgcgcucc ccuucagccg gaccugggac     1800

gacuccuuca acaacaaggu gcugguccug gggagcgaga accagaagaa gcgcaaccag     1860

accccguacg aguaccucga cggcgccucc aacagccagc gguggcgcga guuccaggcc     1920

cggcugcagg gcugccgcuu cuccuacgcc aagaagcagc ggauccagac cgcgaagcug     1980

gacagcaaga cggagcaggg guuccucgag cgcaaccuga acgacacccg guacaucgcc     2040

cgcuuccugu gcggcuucau cgaggagaac cuccaccucc agggggaggg caagcggcgc     2100

guguucgccu ccaacggcca gaucaccgcc cugcugcggg ggcugugggg ccugaagaag     2160

gugcgcgagg agaacgaccg ccaccacgcc cucgacgcga ucgucguggc cugcagcacc     2220

gucuccaugc agcagaagau cacgaaggcc augcagcggc gcgagacccu ggagaccgug     2280

gacaccgaga cgggggaggu gaagacccgg aucccccagc ccugggacuu cuuccgccag     2340

gaggucauga ugcggguguu cagcgacacg ccccacgagg agcuggccga gaagcucucc     2400

ggccgcccgg aggcccugca cgacuucgug agcccccugu ucgucucccg ggcgcccaac     2460

cgcaagauga ccgggcaggg ccaccucgag accgucaaga gcgccaagcg gcugagcgag     2520

aacaucuccg uggugcgcaa gccccugacc gcccucaagc ugaaggacau cgagaacauc     2580

gugggcuacc cggaccggga gcccgcccug uaccaggccc uccgcgagcg gcugcaggcg     2640

uacaaggacg accccgugaa ggccuucgcc gagccguucu acaagcccgc caagaacggg     2700

ggccagggga gccuggucaa ggcggugcgc gugaaggacg uccagaagac cggcgugaug     2760

gugcggaagg accgcggggg caucgcccgg ggcaucgccg acaacgccac caugguccgc     2820

guggacgugu ucgggaagaa gggcaagaac uaccucgucc ccguguacgc cuggcagguc     2880

gcgaagggga uccugcccaa ccgggccgug guggccuacg ccgacgaggc cgacugggac     2940

gagauggacg acuccuacga guucaaguuc acccuguacc cgaacgaccu cguggagauc     3000

aucagcaaga agggccgcau cuucggguac uacuccggcc ugaaccgcgc gacgggcgcc     3060

aucgacauca aggagcacga ccugagcaag gagaaggcca agaacgggau ccaccagucc     3120

gucggcguga agaccgugcu cagcuuccag aaguaccagg ucgacccccu ggggaaggag     3180

aucugccugu gcaaggugga gaagcacccc gagcucaacu ccaagaagaa gaaguga        3237


<210>  1536
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1536
augggcuacc ugaucgggcu cgacaucggc aucaccagcg ugggguacgc cguccugcag       60

accgacuuca agggcaaccc cuacaagauc cugcucauga acacggugau cuucccggug      120

gccgaggcgc ccaagaccgg ggagucccug gccgcccccg uccgccagaa gcggagcucc      180

cgccggacca accgccggac caaguuccgc aagcugcggg ugcagaagcu guucgugcgc      240

uucggccugc uggagagcgc cgacgucgcc acguuccuca accagaaggu gccccaccag      300

gacaucuggc agcugcggaa ggacgcgcug aaccgcaagc ucuccaaccg ggagcuguuc      360

gagauccugu acuucuucgu gggccaccgc ggguucaaga gcaaccggcg cuccgagguc      420

accggcaagc agccgaagga caaccaggug gugcucacca gcauccacga ccgggggaac      480

acccugaacg acuucgacuc ccugggcgag augaugcagg agcuccccga guucaagacg      540

aucaagcaca acaaggccua ccgccccgac aacaccaucu acccccugcg ggccuggauc      600

gagcacgagc ugagcgccau cuucuccguc cagcagaaga acaaccgcca gaucaccgac      660

aacuucauca cccaguaccu cgacaucuuc cggagccagc gcgacuucga ccaggggccg      720

gcggcccccu cccgguucgg cggcaacaug aucgagcgca ugguggggcc cgacagccug      780

gacgcccacg agaagcgcgc cgccaagcug acgaacaccu ucuuccgguu caacuuccuc      840

uccacccuga acaaccugaa gauccagcuc acccugggcg cggaguucca cccccugacg      900

accccgcagc gcgagaucgu gcuccaggcc gccacccuca agagccugaa cuacaagcag      960

auccggcaca agcuccagcu gcccgacucc gcccgcuuca accugguccc cuaccagagc     1020

aacgaggacc ccaucaagac cgaguccaag cacuaccucu ucaagcggaa ggcccugacg     1080

cagaucgagg cggugaccga caacccgcag cugaucgaca agaucgggac cauccucgcc     1140

uacuucaagg gcgacaagac ccgccaggag aagcugagcg cccugaaccu cagccgggcc     1200

gagaucaagc agcugcugcc caucaaccag cccggcuucg ggaaccucuc ccugaagacg     1260

augcacaaga uccugcccua ccucgagcag ggccaccugu accacgaggc cgcgcagcug     1320

gccggguacg acuuccagaa caaccuggug gaccgccagu accuccggga caaccugaag     1380

aacccggucg ugaaccgcgc caucggcaag accaucaagg uggucaacgc caucaucaag     1440

aaguacgggg accccgacgc cauccacauc gagcugaccc gggagaucaa gcacagccac     1500

gaggagcgcc agaagaucgc gcggcagaug aacaccaaca uggaccagaa cgacaaggug     1560

gccgagcacc uccgcgagaa cggcaucccc gugaacggcc agaacaucau caaguccaag     1620

cuguucuacg agcagaaggg gaucgaccug uacagcggcc agcagaucaa cgagcaggac     1680

cucuacacgg aggaccacua ccaggucgac cacaucaucc ccuacuccau cagcuucaac     1740

gacuccuuca ccaacaagac cgugacccgg acgagcaaca accagaacaa ggccaaccgc     1800

cugccgcugg aguaccucuc cgaggagaag caggacgccu uccgggcgcg cgucaugagc     1860

aacaucucca acuaccggaa gcgccagaac cugcugaagg aggggaucac cgaggacaac     1920

cgccagcagu ggaagacccg gaacaucaac gacaccggcu acaucaaccg ccuccugagc     1980

caguaccugc ggaacaacgu gcaguucacg aagaaguccc acaaccccgu guucaccguc     2040

aacggggcca ccaccgccua cauccgcaag cgguacggca ucaucaagaa ccgccaggcc     2100

accgaccucc accacgccgu ggacgcggug aucaucggcu gcaucagcaa cacguucguc     2160

ucccggcugg ccaaguacag cgaggcccag gaggcccgcu accacaagca gcuguggcag     2220

aucacccagu accggcagga gcacaaggac cucuccacca ucgaguacca ggagcugauc     2280

aagcaguucc cccugcccug gccggacuuc cgccacgagc ucauggcccg gcugagcacc     2340

gaccccgcga agaugauggc ccaccacacg ugggagcacu acaccgacga ggagaucucc     2400

cagcugaagc ccgccuucgu gauccgcagc gccaaccaca aggugaccgg gagcgcccac     2460

gacgacacca ucuacuccaa gaagcucuac gaccagcggg gcgucgggcu gcugcgcacg     2520

agccuccaga accugaagua cgacuccaag aaggacggca ucaagaacua cgagcggcug     2580

aaggacggga gcaacucccu cguguacgac agccugcugc gcgcgcucaa ggagcacgac     2640

ggcaacgcca agaaggccuu cccggacaac cagcugcuga ucuccaccgg caaccaccag     2700

cagaucauca cccacgugaa gaccgucaag aagaucacgc accccaucga ggugaaccgg     2760

gacaccgcca ucgcgaucgg gggccgcaug auccgggugg acaucuucaa gcaggccaag     2820

gcccagaagc ucaucgggau ccccgucuac gugaacgaca ccgugaagcc cacccugccg     2880

aaccgcgccu gcacgagcgg caagcgguac uccgacuggu accagcugac ccgccaggac     2940

caguuccucu gcagccuggu cuccaacgac cuggugcucg uggagaccgc cagcccccug     3000

aaggucacca acaacaucac gaacgaggag gugcagacca cccagcugcu cugcuacuuc     3060

uccagcuuca acaucgcgaa cgcccagauc aacuuccacu ccaucaacaa cgacagcucc     3120

aucaacccca ucgugcugaa caaggucacc uaccugacga aguaccagcu cgacuaccug     3180

ggcaacuacu uccccgugaa gcacgagagc cgcaagaccu ucaaccggaa gcgcuga        3237


<210>  1537
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1537
augcacaccc gccuggcccu cgacaucggc accacgagca ucggguggau ccuguacgag       60

cuggugaacg gccagcccgc cuccauccuc gacgggggca cccggaucuu cagcgacggg      120

cgcgaggcga agaccggcca cucccuggcc gucggccggc gcaccgcccg ggggaugcgc      180

cggcgccggg accgcuaccu gcggcgccgg gccgcccuca ugaagcgccu ggcggccauc      240

ggccugaugc cggagagccc cgaggccgcc gcgcagcucg ccacgcugga ccccuacgag      300

cugcgggccc gcgggcucga ccacccccug ccgcccgagc accugggccg ggcccucuuc      360

caccugaacc agcgccgggg guucaagucc aaccgccgga ccgacaaggg caacaacgag      420

ggcgggauga ucgccgacgc gagcgcccgc cuggagcagg ccaugauggc cgccggcgcg      480

cgcuccuacg gggaguuccu ccacaagcgg cgcgccgagg ccaccgcgcc ccgggccacc      540

cccgccgugc gcacgcggau caacccgcac cugaccggcg aggacggcaa ggagcagccc      600

ggguacgacu ucuaccccga ccgcaagcac cuggaggacg aguuccaccg gcucugggag      660

gcccaggccc gccacaaccc cgaccugacc ccggccaugg gcgaccuccu guuccagacc      720

aucuuccacc agcggccccu gaaggccccc gcgguggggc gcugccuguu ccucgacgag      780

ccccggcugc cgaaggccca cccccugacg cagcagcgca cccucuacga gaccgucaac      840

augcugcgga ucgccccgcc cggccagccc aagcgccggc ugagccgcgu gcagcgggac      900

cagaucgugc acgcccucaa caaccgcaag auccuggcgu cccccgggaa gaacguccug      960

acccucgccg ccaucggcaa gcugcugaag cuccggcccg gcgaggccuu cagccuggac     1020

acggccaacc gcgacgcgau cgccugcgac cccgugcgca ugucccuggc cuacgagggg     1080

cgguucggcc cgcaguggag cgcccucucc guggagagcc agugggagcu gauccagcgc     1140

cugcgggacg accaggacgg ggccgcgcuc gucgacugga ucauggaccg cuacggcuuc     1200

gaccgggacc gcgcccgggc caucgccgac gcgccccugc ccgagggguu cggccgccug     1260

ggcgagaccg ccacccgggc cgugcucgcc gugcugaucg ggggcaccac ggagcaggag     1320

guccugaccu acuccgaggc cguggcggcc ugcggguggg accacagccg ccugggcacc     1380

ggggaggugc ucgaccggcu gcccuacuac ggccaggucc uggaccgcca cgugcucccg     1440

ggcaccgggc ggcccgagga cgacgagauc acgcgcuucg gccggaucac caaccccacc     1500

gugcacaucg ggcugggcca ggucaagcgc cuggugaaca aggugaucga cgccuggggc     1560

cggccgcacg agauggucgu ggagcucgcc cgcgaccuga agauguccga ggaccagaag     1620

cggaaccugg ccaaggugaa cgcgcagaac cagcgcgccg ccgagaugcg gagcgccaag     1680

cucaucgggg accugaagau cgaggacaac ggccgcaacc gcgggauccu gcgggucugg     1740

gaggaguccg ccgcggacgu gcuccaccgc ugcugccccu acaccggcgc ccagaucagc     1800

gccagcaugc uguucgacgg guccugcgac aucgaccaca uccugcccua cagccggacg     1860

cucgacgacg gcuucaacaa ccgcgucauc ugccugaagu ccgccaaccg ggagaagcgc     1920

aaccugaccc ccugggaggc cuggggcaac accccgaagu gggcggccau cgaggccaac     1980

cucaagaacc ugccgcccgc caaggcgugg cgguucgccc ccgacgccau gcugcgcuac     2040

gagggggagc gggacuucac cgcccgcgcc cucguggaca cgcaguaccu gagccggcug     2100

gcgcgcgagu accucgagug ccuguacgcc gaggacaucg accccggccc ccacuccggg     2160

aagaagcccc ggcgccccau cugggugguc accggcaagc ugaccgagcu ccugcggcgc     2220

cacugggggc ugaacagccu ccugccggac gcccaccggg gcaccgccaa ggccaagaac     2280

cgccuggacc accggcacca caugaucgac gcggccgugg ugggcgccac ggaccacgcc     2340

accguccagc gcaucgccaa cauggcgaag gggaacgugg agaacggccu cgaggaccug     2400

gccgggacca uccugccgcc cuggcccggc cuccgcgacg accugaaggc ccggcuggag     2460

cgcaccaucg ugucccaccg ggggaaccac ggcgccaucg accccgccgc gcgcaagcag     2520

ggccgggaca ccacgagcgg gcagcuccac aacgacaccg ccuacggccu gaccggggag     2580

acccugaagg gcgucccccu cguggugaac cgcaagccca ucgacaugcu gacgcccgcc     2640

augaucgaca agauccggga cccgcaccug ucccgcuggc ucaccgccgu caccaagggg     2700

aaggagggca aggacuucaa ggcggccgug gaggccuucc gggcccaccc cggccccuac     2760

cgcgggaucc ggcgcgugcg gcugaucgag cccgucgagg ugauccgcau caaggaccgg     2820

aacggccgcc cguacaaggg guacaagggc gacagcaacc agugcuacga gguguggugc     2880

cugcccgacc ggacccucac gcaccacguc guguccaccu ucgccgcgca ccagggccag     2940

accgggaagc cccacccggc cgccaagcgc cuggcccggc uguacaaggg cgacauggug     3000

cgccucgacc ggagcaaguu cggguccguc aucgccaccg uggagaaguu cgaccgcaac     3060

ggccagcugg agcuggugcc ccacaacgag gggaacgcgg accagcgcua ccggaaggac     3120

aagagcgacu ccgucuucau ccgccuccgg gccgccacgu ucaucgccag cggcgcccgc     3180

cgggugaucg uggacgagau gggccgcauc cgggaccccg ggcccggcaa gcacuga        3237


<210>  1538
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1538
augaaguacg gcaucgggcu ggacugcggc aucaacagcg ugggguacug caucauggag       60

cucaacuccg acgacgagcc caagcgcauc guccggcuga acagccgcau cuucaccgag      120

gccgagaacc cgaaggacgg cuccagccug gccgcgcccc ggcgcgaggc ccgggggcuc      180

cgccggcgca uccggcgcca caagcaccgg cuggagcgca uccgggcccu gcucgugcgc      240

gagaacaucg ugacccaggc cgagcuggac aacauguucu ccggcacgcu gagcgacauc      300

uacgagcucc ggaccgccgc gcuggacgcg ccccugaaca acaucgaguu cgcccgcguc      360

cucaucaacc uggcccagcg gcgcggcuuc aaguccaacc gcaagagcga gaaguccagc      420

uccgacaagg agaccgggaa gcugcucagc gcgaucaaga agaacaagga guccaucgag      480

gccggcgggu accggaccgu gggcgagaug cuguucaagg acgagcggua cgccaagcac      540

aagcgcaaca agggggaggc cuaccugaac acggugagcc gcgacaugau cgaggccgag      600

auccacgcgg ucuucgccuc ccagcggaac uucggcaugc ccuucgccaa cgagaagauc      660

gagaacgagu acaccgacau cgcccucagc cagcgcccgu ucgaccuggg ccccggggag      720

ggccccgaga acugcaaguc ccccuacgcc gggaagcaga ucaagaacau gaucggccag      780

ugcaccuucu ucccggagga gaagcgggcg gugaaggcca gcuacuccuu ccagcuguuc      840

agccuccugc agggcaucaa caacaucacc cuggugagcu ccgacgggaa cacguucccc      900

cucagccagg agcagcgcaa gacccugaag gacuacugcu ucaagaccgc caacgucacc      960

uacgccucca uccggaaggu gcugcagauc ccccaggagu acacguucaa gaacaucacc     1020

uacaccgacg acggcaucga gaaguccgag aagaagacca aguuccagca ccucaagccc     1080

uaccacgaga ugaagaaggu gcuggggagc gccauccaga cgcugacccc ggaggagcuc     1140

gacgagaucg gcgagaucuu caccuucuac aaggacgaga ccaucaucau caagcagcug     1200

gaggagacgg agaucgacaa gagccuguac gaccuccugc ugcagcuccc caccuucgag     1260

aaggcggggc acaucuccac caaggccugc aagaugauca uccccuuccu ggaggagggc     1320

aagaccuacg acaaggccug cgagugcgcc ggccugaacu ucaaggccca cacgaacaug     1380

cagaagcaga ugcuccugcc cgccaagagc gacgagcuga acgacgucgu gaacccggug     1440

guccgccggg ccaucuccca gaccaucaag guggugaacg ccaucauccg cgagaugggg     1500

accagcccca ccuacaucaa caucgagcuc gcccgggagc uguccaagag caagaaggag     1560

cgcgacgaga ucaacaagaa guacaagcug aaccaggcgg agaacgagcg gcucaagaac     1620

gagaucaucg acaacuucaa gaucaagccc aacggccagg acaucaucaa gcugaagcug     1680

uggcacgagc aggacgacgg gaucugcccc uacacgggcg uccgcaucaa guacgagaac     1740

cucuucgacg acggguacgc cgagaucgac cacaucaucc cguacucccu gagcuucgac     1800

gacuccuuca acaacaagau ccugaccacc gccacccaga accggcagaa gggcaaccgc     1860

aucccccugg aguaccuccc cgagagcgag aaggacggcu uccgggugug ggugaacucc     1920

aacauccgca accaccggaa gcgccagaac cugcugaagg agcacuucac ggaggccgac     1980

aagaaggagu ucaagagccg gaaccucaac gacacccgcu accugaacaa gcugcuccug     2040

aacuacauca acgacaaccu ccuguucacc gacuucuaca acgggaagaa gaagcacguc     2100

accuccguga acggcgccgu gacggcguac auccgcaagc ggugggggau caacaagaac     2160

cgcgaggacg gcgaccugca ccacgccguc gacgccgccg ugaucgccug cgucacccag     2220

gggaucauca accggaucac cgcguacagc gcccgcaacg aggcccccua cgaccugaac     2280

accgagaccg gcgagaucaa ggagcgguuc ccgcuccccu accccaacuu caagaaggag     2340

cuggacgccc gcacgaccau cgaggacgag aagcggcuga agcgcgugcu caugagcuuc     2400

uccaacuaca gcuacgacga cguggagaag gccaagcccu gcuucgucuc ccggaccccg     2460

caccgcaagg ugaccggcgc ggcccacgag gagacgaucc ggagcgggaa gguggagggc     2520

uucaccgucu ccaagaccga gcugaccaag cugaagcuca cgaacgggga gaucgagggc     2580

uacuacgagc ccgagagcga cacccugcug uacaacgccc uccugcagcg ccugaacgag     2640

uucggcgggg acgccaaggc ggccuucgcc gacgaguucc acaagcccaa ggccgacggc     2700

accccggggc ccguggugaa gaaggucaag accaucaagc gguccagcuc caacgugaag     2760

gcccgcaaca agaacggccu cgggguggcg gacaacggca gcaugauccg gaucgacguc     2820

uucuacgugg agaacgaggg cuacuacuuc gugcccaucu acgucgccga cacggugaag     2880

gagaugccca acgaggccug cccguucggg aaggacaaga agaagaucau gaccgacgac     2940

gacuuccugu ucucccugua ccccaacgac cucaucaaga ucaccgccaa gaaggacaug     3000

aaguucagcc uggccaacaa ggaguccggc cugcccaaga acaaguacgc gaacgaggug     3060

cucgucuacu acagcgccgc cgacaucucc aacgccagca ucuccgugau caugcacgac     3120

aacgccuaca aguuccgcgg gugcggcauc aagagccugg ugaagcugga gaaguacacc     3180

gucgaccccc ucgggaacgu gagcaaggug aaccgggaga agcgcaugua cuucaaguga     3240


<210>  1539
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1539
augcgccugg gccucgacau cgggaccagc uccaucggcu gguggcugua cgagaccgac       60

ggggccggca gcgacgcccg gaucacgggg guggucgacg gcggcgugcg caucuucucc      120

gacgggcggg accccaagag cggcgcgucc cuggccgugg accgccgggc cgcccgcgcc      180

augcggcgcc ggcgcgaccg guaccuccgc cggcgcgcga cccugaugaa gguccuggcc      240

gagaccgggc ucaugccggc cgaccccgcc gaggcgaagg cccuggaggc ccuggacccc      300

uucgcccucc gggccgcggg ccuggacgag ccccugccgc ucccccaccu ggggcgcgcc      360

cuguuccacc ucaaccagcg gcgcggcuuc aagagcaacc ggaagaccga ccgcggcgac      420

aacgaguccg ggaagaucaa ggacgccacg gcccggcugg acauggagau gauggccaac      480

ggcgcgcgca ccuacgggga guuccugcac aagcgccggc agaaggccac cgacccccgc      540

cacgugccca gcgugcggac ccgccucucc aucgccaacc ggggcggccc ggacgggaag      600

gaggaggccg gcuacgacuu cuaccccgac cgccggcacc uggaggagga guuccacaag      660

cugugggccg cgcagggggc ccaccacccc gagcucacgg agacccugcg cgaccuccug      720

uucgagaaga ucuucuucca gcggccccug aaggagccgg aggucggccu gugccucuuc      780

agcgggcacc acggcgugcc gcccaaggac ccccgccugc ccaaggccca cccccugacc      840

cagcggcgcg ugcucuacga gaccgucaac cagcugcggg ugacggccga cggccgcgag      900

gcccggcccc ugacccgcga ggagcgggac caggugaucc acgcgcucga caacaagaag      960

cccaccaagu cccugagcuc caugguccug aagcucccgg cccuggccaa ggugcugaag     1020

cuccgcgacg gggagcgguu cacccuggag acgggcgugc gcgacgccau cgcgugcgac     1080

ccccugcgcg ccagccccgc ccaccccgac cgguucgggc cgcgcugguc cauccucgac     1140

gccgacgccc agugggaggu caucagccgg auccgccggg ugcaguccga cgcggagcac     1200

gccgcccugg uggacuggcu gaccgaggcc cacggccucg accgcgccca cgcggaggcc     1260

accgcccacg cgccccugcc cgacggguac ggccggcugg gccucaccgc cacgacccgc     1320

auccuguacc agcugaccgc cgacgucgug accuacgccg acgccgugaa ggccugcggg     1380

uggcaccaca gcgacggccg gacgggggag ugcuucgacc gccugcccua cuacggcgag     1440

guccucgagc ggcacgugau cccggggucc uaccaccccg acgacgacga caucacccgc     1500

uucggccgga ucaccaaccc caccgugcac aucggccuga accagcugcg ccggcucguc     1560

aaccgcauca ucgagacgca cgggaagccg caccagaucg uggucgagcu ggcgcgggac     1620

cugaagaagu ccgaggagca gaagcgcgcc gacaucaagc ggauccgcga caccaccgag     1680

gccgccaaga agcggagcga gaagcucgag gagcuggaga ucgaggacaa cggccgcaac     1740

cgcaugcugc uccggcugug ggaggaccug aaccccgacg acgccaugcg ccgguucugc     1800

cccuacaccg ggacgcgcau cagcgcggcc augaucuucg acggcagcug cgacguggac     1860

cacauccucc ccuacucccg gacccuggac gacagcuucc cgaaccgcac ccugugccuc     1920

cgggaggcca accgccagaa gcggaaccag acccccuggc aggccugggg cgacacgccc     1980

cacuggcacg ccaucgcggc caaccugaag aaccugcccg agaacaagcg cuggcgguuc     2040

gccccggacg ccaugacccg cuucgagggg gagaacggcu uccucgaccg ggcccugaag     2100

gacacccagu accuggcgcg caucucccgg agcuaccucg acacccuguu caccaagggc     2160

gggcacgugu gggucgugcc cgggcgcuuc acggagaugc ugcggcgcca cuggggccuc     2220

aacucccugc ugagcgacgc cggccgcggg gccgugaagg ccaagaaccg gaccgaccac     2280

cgccaccacg cgaucgacgc cgccgucauc gccgccaccg accccggccu ccugaaccgg     2340

aucucccgcg cggccgggca gggcgaggcc gccgggcaga gcgccgagcu gaucgcgcgg     2400

gacacgcccc cgcccuggga gggcuuccgc gacgaccucc gggugcgccu ggaccggauc     2460

aucguguccc accgcgccga ccacggccgg aucgaccacg ccgcccgcaa gcaggggcgg     2520

gacagcaccg ccggccagcu gcaccaggag accgcguacu ccaucgucga cgacauccac     2580

guggccagcc gcaccgaccu ccugucccug aagcccgccc agcuccugga cgagcccggg     2640

cggagcggcc aggugcgcga cccgcagcug cggaaggccc uccgcgucgc caccgggggc     2700

aagacgggca aggacuucga gaacgcgcug cgguacuucg ccuccaagcc cgggcccuac     2760

caggccaucc gccgggugcg caucaucaag ccccugcagg cccaggcgcg ggugccgguc     2820

cccgcccagg accccaucaa ggccuaccag ggcgggagca accaccucuu cgagaucugg     2880

cgccugcccg acggcgagau cgaggcccag gugaucaccu ccuucgaggc ccacacccug     2940

gagggggaga agcgcccgca ccccgcggcc aagcggcucc ugcgcgugca caagggcgac     3000

auggucgccc uggagcggga cggccgccgg gugguggggc acguccagaa gauggacauc     3060

gccaacggcc ucuucaucgu gccccacaac gaggccaacg cggacacccg caacaacgac     3120

aagagcgacc cguucaagug gauccagauc ggggcccggc ccgccaucgc cagcggcauc     3180

cgccgggugu ccgucgacga gaucggccgc cugcgggacg ggggcacgcg ccccaucuga     3240


<210>  1540
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1540
auggacgagg aggccaagau ccagcugagc aaggaguccu acgaggagga gcucgagagc       60

cugcuggaga aguccggcga ggaguuccgc gacguguucc uccaggccaa gaaggucuac      120

gacgcgaucc ugcugagcga cauccucucc accaagaagc agaacagcaa ggccaagcug      180

ucccugggga ugaucgagcg guacgacagc cacaagaagg accucgagga gcugaagcag      240

uucgugaagg ccaaccugcc cgagaagacc gccaucuucu ucaaggacuc cagcaagaac      300

ggcuacgccg gguacaucga cggcaagacg acccaggagg acuucuacaa guuccucaag      360

aaggagcuga acgggaucgc gggcuccgag cgcuucaugg agaaggugga ccaggagaac      420

uuccugcuca agcagcggac caccgccaac ggcgucaucc cgcaccaggu gcaccugacg      480

gagcugaagg ccaucaucga gcgccagaag cccuacuacc ccuuccucga ggaggcccgg      540

gacaagauga uccgccugcu gaccuuccgg auccccuacu acguggggcc gcucgcgcag      600

ggcgaggaga ccagcuccuu cgccuggcug gagcgcaaga cccccgagaa ggucacgccc      660

uggaacgcca ccgaggugau cgacuacagc gccuccgcca ugaaguucau ccagcggaug      720

aucaacuacg acaccuaccu gcccaccgag aaggugcucc cgaagcacag cauccuguac      780

cagaaguaca cgaucuucaa cgagcugacc aaggucgcgu acaaggacga gcgcgggauc      840

aagcaccagu ucuccagcaa ggagaagcgg gagaucuuca aggagcuguu ccagaagcag      900

cgcaagguga ccgugaagaa gcugcagcag uuccuguccg ccaacuacaa caucgaggac      960

gccgagaucc ucggcgucga caaggccuuc aacagcagcu acgccaccua ccacgacuuc     1020

cuggaccugg cgaagcccaa cacggagcgg guggccgagc uccuggagca gcccgagaug     1080

aacgccaugu ucgaggacau cgugaagauc cugaccaucu ucgaggaccg cgagaugauc     1140

cggacccagc ucaagaagua ccaguccguc cugggggacg gcuucuucaa gaagcuggug     1200

aagaagcacu acaccggcug ggggcgccuc agcgagcggc ugaucaacgg cauccgcgac     1260

aagaagacga acaagaccau ccuggacuac cucaucgacg acgacgacuu ccccuacaac     1320

cggaaccgca acuucaugca gcugaucaac gacgacuccc ugagcuucaa ggaggagcuc     1380

gccaacgagc uggcccuggc ggggaaccag ucccuccugg agguggucga ggcccugccg     1440

ggcagccccg ccaucaagaa gggcaucugg cagacccuga agaucgugga ggagcucauc     1500

gagaucaucg gguacaaccc caagaacauc guggucgaga uggcccggga gaaccagcgc     1560

accaaccgcu ccaagccccg gcugaaggcc cuggaggagg cgcucaagag cuucgacucc     1620

ccgcugcuga aggagcagcc cguggacaac caggcccucc agaaggaccg ccuguaccug     1680

uacuaccucc agaacggcaa ggacauguac acgggggagg cccuggacau cgaccggcug     1740

agcgaguacg acaucgacca caucaucccc cgcuccuuca ucguggacaa cagcaucgac     1800

aacaaggucc ucguguccag caaggagaac cggcugaaga uggacgacgu ccccgaccag     1860

aaggugguga uccgcaugcg gcgcuacugg gagaagcugc uccgggccaa ccugaucucc     1920

gagcgcaagu ucgcguaccu gaccaagcuc gagcugaccc cggaggacaa ggcccgguuc     1980

auccagcgcc agcuggucga gacccggcag aucacgaagc acguggccgc cauccucgac     2040

caguacuuca accagcccga ggagagcaag aacaagggca uccgcaucau cacccugaag     2100

uccagccugg ugucccaguu ccggaagacc uucgggaucc acaagguccg cgagaucaac     2160

aaccaccacc acgcccacga cgcguaccuc aacggcgugg uggccaucgc ccugcugaag     2220

aaguacccca agcucgagcc cgaguucguc uacggcaacu acaccaaguu caaccuggcc     2280

acggagaaca aggccaccgc gaagaaggag uucuacagca acauccugcg guucuucgag     2340

aaggaggagu acagcuacga cgagaacggg gagaucuucu gggacaaggc ccgccacauc     2400

ccgcagauca agaaggugau cuccagccac caggugaaca ucgucaagaa gguggaggug     2460

cagaccggcg gguucuacaa ggagaccguc aaccccaagg gcaagcccga caagcucauc     2520

cagcggaagg ccggguggga cguguccaag uacggcggcu ucgggagccc cguggucgcc     2580

uacgccgugg cguucaucua cgagaagggc aaggcccgca agaaggccaa ggccaucgag     2640

gggaucacga ucaugaagca gucccuguuc gagcaggacc cgaucggcuu ccugagcaac     2700

aagggguacu ccaacgugac caaguucauc aagcucagca aguacacccu guacgagcug     2760

gagaacggcc gccggcgcau ggucgcgucc cacaaggagg cccagaaggc caacagcuuc     2820

auccuccccg agaagcuggu gacccuccuc uucuacgccc agcacuacga cgagaucgcc     2880

cacaaggagu ccuucgacua cgugaacgac caccugagcg aguuccggga gauccuggac     2940

caggucaucg acuucuccaa ccgcuacacc aucgcggcca agaacacgga gaagaucgcc     3000

gagcuguucg agcagaacca ggagagcacc gugcaguccc ucagccaguc cuucaucaac     3060

cugaugcagc ucaacgccau gggcgccccc gcggacuuca aguucuucga cgugaucauc     3120

ccgcggaagc gcuaccccag ccugaccgag aucugggaga gcaccaucau cuaccagucc     3180

aucacggggc ugcgggagac ccgcacccgg auggccaccc ucugggacgg cgagcaguga     3240


<210>  1541
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1541
auggacgagg aggccaagau ccagcugagc aaggaguccu acgaggagga gcucgagagc       60

cugcuggaga aguccggcga ggaguuccgc gacguguucc uccaggccaa gaaggucuac      120

gacgcgaucc ugcugagcga cauccucucc accaagaagc agaacagcaa ggccaagcug      180

ucccugggga ugaucgagcg guacgacagc cacaagaagg accucgagga gcugaagcag      240

uucgugaagg ccaaccugcc cgagaagacc gccaucuucu ucaaggacuc cagcaagaac      300

ggcuacgccg gguacaucga cggcaagacg acccaggagg acuucuacaa guuccucaag      360

aaggagcuga acgggaucgc gggcuccgag cgcuucaugg agaaggugga ccaggagaac      420

uuccugcuca agcagcggac caccgccaac ggcgucaucc cgcaccaggu gcaccugacg      480

gagcugaagg ccaucaucga gcgccagaag cccuacuacc ccagccucga ggaggcccgg      540

gacaagauga uccgccugcu gaccuuccgg auccccuacu acguggggcc gcucgcgcag      600

ggcgaggaga ccuccagcuu cgccuggcug gagcgcaaga cccccgagaa ggucacgccc      660

uggaacgcca ccgaggugau cgacuacucc gccagcgcca ugaaguucau ccagcggaug      720

aucaacuacg acaccuaccu gcccaccgag aaggugcucc cgaagcacuc cauccuguac      780

cagaaguaca cgaucuucaa cgagcugacc aaggucgcgu acaaggacga gcgcgggauc      840

aagcaccagu ucagcuccaa ggagaagcgg gagaucuuca aggagcuguu ccagaagcag      900

cgcaagguga ccgugaagaa gcugcagcag uuccugagcg ccaacuacaa caucgaggac      960

gccgagaucc ucggcgucga caaggccuuc aacagcuccu acgccaccua ccacgacuuc     1020

cuggaccugg cgaagcccaa cacggagcgg guggccgagc uccuggagca gcccgagaug     1080

aacgccaugu ucgaggacau cgugaagauc cugaccaucu ucgaggaccg cgagaugauc     1140

cggacccagc ucaagaagua ccagagcguc cugggggacg gcuucuucaa gaagcuggug     1200

aagaagcacu acaccggcug ggggcgccuc uccgagcggc ugaucaacgg cauccgcgac     1260

aagaagacga acaagaccau ccuggacuac cucaucgacg acgacgacuu ccccuacaac     1320

cggaaccgca acuucaugca gcugaucaac gacgacagcc uguccuucaa ggaggagcuc     1380

gccaacgagc uggcccuggc ggggaaccag agccuccugg agguggucga ggcccugcug     1440

ggcuccccgg ccaucaagaa gggcaucugg cagacccuca agaucgugga ggagcugauc     1500

gagaucaucg gguacaaccc caagaacauc guggucgaga uggcccggga gaaccagcgc     1560

accaaccgca gcaagccccg gcugaaggcc cucgaggagg cgcugaaguc cuucgacagc     1620

ccccugcuca aggagcagcc gguggacaac caggcccugc agaaggaccg ccuguaccuc     1680

uacuaccugc agaacggcaa ggacauguac acgggggagg cccuggacau cgaccggcuc     1740

uccgaguacg acaucgacca caucaucccc cgcagcuuca ucguggacaa cuccaucgac     1800

aacaaggucc uggugagcuc caaggagaac cggcugaaga uggacgacgu ccccgaccag     1860

aaggugguga uccgcaugcg gcgcuacugg gagaagcucc ugcgggccaa ccugaucagc     1920

gagcgcaagu ucgcguaccu caccaagcug gagcugaccc ccgaggacaa ggcccgguuc     1980

auccagcgcc agcucgucga gacccggcag aucacgaagc acguggccgc cauccuggac     2040

caguacuuca accagccgga ggaguccaag aacaagggca uccgcaucau cacccucaag     2100

agcucccucg ugagccaguu ccggaagacc uucgggauca acaagguccg cgagaucaac     2160

aaccaccacc acgcccacga cgcguaccug aacggcgugg uggccaucgc ccugcucaag     2220

aaguacccca agcuggagcc cgaguucguc uacggcaacu acaccaaguu caaccuggcc     2280

acggagaaca aggccaccgc gaagaaggag uucuacagca acauccuccg guucuucgag     2340

aaggaggagu acuccuacga cgagaacggg gagaucuucu gggacaaggc ccgccacauc     2400

ccccagauca agaaggugau cagcucccac caggugaaca ucgucaagaa gguggaggug     2460

cagaccggcg gguucuacaa ggagaccguc aacccgaagg gcaagcccga caagcugauc     2520

cagcggaagg ccggguggga cgugagcaag uacggcggcu ucgggucccc cguggucgcc     2580

uacgccgugg cguucaucua cgagaagggc aaggcccgca agaaggccaa ggccaucgag     2640

gggaucacga ucaugaagca gagccuguuc gagcaggacc ccaucggcuu ccucuccaac     2700

aagggguaca gcaacgugac caaguucauc aagcugucca aguacacccu guacgagcuc     2760

gagaacggcc gccggcgcau ggucgcgagc cacaaggagg cccagaaggc caacuccuuc     2820

auccugccgg agaagcuggu gacccuccug uaccacgccc agcacuacga cgagaucgcc     2880

cacaaggaga gcuucgacua cgugaacgac caccuguccg aguuccggga gauccucgac     2940

caggucaucg acuucagcaa ccgcuacacc aucgcggcca agaacacgga gaagaucgcc     3000

gagcuguucg agcagaacca ggaguccacc gugcagagcc ugucccagag cuucaucaac     3060

cucaugcagc ugaacgccau gggcgccccc gcggacuuca aguucuucga cgugaucauc     3120

ccccggaagc gcuacccgag ccugaccgag aucugggagu ccaccaucau cuaccagagc     3180

aucacggggc uccgggagac ccgcacccgg auggccaccc ugugggacgg cgagcaguga     3240


<210>  1542
<211>  3243
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1542
augaagacca gcuucgccuu cgacaucggc accaacagca ucggguggug cgugcuggcc       60

cucgacaacg cgaaccugcc cuaccgcauc cuggacgccg gcguccggau cuucagcgac      120

gggcgcgagc cgaaguccgg cacgagccuc gccgaggggc ggcgccuggc ccggggcaug      180

ucccgccggc gcgaccggua caagcgccgg cgcaaggccg ugauccggac ccugaucgag      240

uacggccuca ugcccgacga ccccgcggcc cgccgggccc ugguggccga gaccaacgac      300

aaggcgcccg ggaagaucgc cagcgacguc uacgcccugc gcgcccgggc ccucgacgac      360

aagcugccgc ugcaccacau cggccgcgcc cucuuccaca ucaaccagcg gcgcggguuc      420

aaguccaacc ggaagaccga caagcgcgac aacgaccagg gcaagaucgc cagcgggauc      480

ucccgccugc gggacaagcu ggccagcgag ggcaugcgca cgcucggcga guaccugaac      540

caccggcacc aggccgggaa cugggugcgc gugcggcccu cccgcguccc cggcaaggac      600

gacaaggagg aggacgggua cggcuucuac cccgagcggg cccugcucca ggacgaguuc      660

aacgcgaucu ggaccgccca ggccgcccac uacccggacg ugcugaccga ggagcgccgg      720

acccaccugg agacgaucau cuucuuccag cgcccccuca agcccgugca ggucggcaag      780

ugcagcuuca accccgggga ggagcggcug ccgcgcgccc acccccuguu ccaggaguuc      840

cggcucuaca aggaggugaa cgagcuggag cugguggacc gcaaccagga ccaccacaag      900

cucucccugg accagcggga cgcgcugauc gcccagcuca agacccagcg cgaggccgcc      960

uacagcgccc ugcggaagac cgcgcgcgcg ccccugggca cccgguucaa caaggagacg     1020

gagucccgca ccaagaugaa gggggacgag gucgccgccg agcucagcgc gaagacccgc     1080

uucggccagg ccuggaucuc caugccccug gaggagcggu ggaucaucgu ggacacccug     1140

cgccgggugg aggacccgcu cgagcugacg gccuggcugg agcagcacua cgggcucgac     1200

ggcgagcaca ucgacgccau ccuggccgcg cgccugcccg agggcuacgg gcggcucggc     1260

gagaccgccc ugaccgagau ccuggucgag cucaaggccg ccgugaucag cgaggccgag     1320

gcggcccgcc gggccgggua cgaccacgcc cugcugcagc gcggcgccgg gcucgaggcg     1380

cugcccaagu accaggaggu gaucgcccgg cgcaucccgc ccggcaccgg caaccccgac     1440

gaccccuacg acgucgagaa ggggcggauc acgaacccca ccgugcacau cggccugaac     1500

cagcugcaga aggugcucaa cgcccugauc cgccgguacg ggaagccgga gcagaucgcc     1560

aucgagcugg cgcgcgagcu caagcugaac gacgagcaga agcgggagaa gaacaucgag     1620

aucgcccgca acacccgggc cgccgagcag cgcuccgcca agcuggucga gcucggcgug     1680

cccgacaccg gguacaaccg ggugcugcug aagcucuggg aggagcugaa ccugcgcgcg     1740

cccgagaacc gggucugcau cuacugcggc gagcccauca acaucgagac ccucuucagc     1800

agcgccgugg acaucgacca cauccugccg ugguccaaga cgcuggacga cagccaggcc     1860

aaccgccucg ucugccacgu ggccugcaac cgccagaagg ccaacuacgc gcccgccgag     1920

gugccccagu ggcagggccg guacgacgac auccuggccc gcgccaacac ccugccgcuc     1980

aacaagcggu ggcgcuucgg gcgggacgcc augaagaagu ucgcggacga ggagaccuuc     2040

cuggcccgcc agcugacgga cacccaguac cucucccggc uggcccacgc cuacauggcg     2100

gcccuguacc ccgacgagga gcccgacgcc cagggcgucc ucgcccgcaa gaaccacgug     2160

cgggugauca ccgggcgcau gaccgagaug cugcggcgcc aguggggccu gaacagccuc     2220

cuggccgacc acaaccagaa gaaccggaac gaccaccgcc accacgcgau cgacgccgcc     2280

gucgugggcg ugacgacccg ggcccugcuc cagcgcaucg ccaccgucgc cgggggccgg     2340

gaggcccagg cccuggagga gaccaucggg aagaucgagc ccccguggcc cggcuuccgc     2400

gacgagcugc aggccgugau caagcggauc guggucuccc acaagcccga ccacgggacg     2460

cuccccaagc cgggccaggg cgggcagacc gccggccagc ugcacaacga caccgcguac     2520

gggcugaccg gcgagaccga cagccgcggg acgcccaucg uggugcgccg gaagcccuuc     2580

accucccuca ccgagaagga ccugcccagc auccgcgacc ggcagcuggc ccaggcccuc     2640

ggcuccgcca ucgccggccg cagcgggaag gagcugcagg cggcccugga gcaguuccgg     2700

gaccgcgacg acuacaaguc cauccggcac guccgcgugg uggagccgcu caacgucauc     2760

cccauccggg acggcaccgg gcgcgccuac aaggccuaca agggcgacgc gaacuaccgg     2820

uacgacgugu gggagcugcc caacgggaag uggaugcacg agguggucag cauguucgac     2880

gcccacgacc gcuccuggac gagcgccgug cacauccacc accccgccgc ccggcgcgug     2940

cugucccuca agcagaacga cauggucgcg uacgaccacc cgcaggacgg ccccaccauc     3000

ggccgggugg ugaaguucag ccaguccggg cagaugaccu ucgcgcccca caaggaggcc     3060

ggcgcccuga aggcccgcga cgcggacccc gaggaccggu uccgcuacuu cuacaagagc     3120

ggguccgcca ugaaggacau cggccugcgg cagguccgcg uggacgagau cgggcgggug     3180

uucgacccgg gcccccgcga caaggaggcc cgcaacgccc ggaagaagaa guccaccgac     3240

uga                                                                   3243


<210>  1543
<211>  3243
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1543
auggccaaga gcuucgccuu cgacaugggc accaacucca ucgggugggc ggugcugggc       60

cucgacagcg ccggggaccc cgacuccauc gagggcgccg ggguccgcau cuucagcgac      120

ggccgggagc cgaaguccgg cgccagccug gccgaggggc gccggaucgc gcgcggcaug      180

ucccggcgcc gggagcgcua caaggcccgg cgcaaggccc ugauccggac ccucggggac      240

uacggccuga ugccccugga gcgcgccgcg cggcgcgccc ucguggccga gacgaacgac      300

cggagcuucg ggcugguggc ccaggacguc uacgcccugc gcgcgcgggc ccucguggag      360

aagcugccgc ccuaccaccu gggccgcgcc cucuuccacc ugaaccagcg gcgcggcuuc      420

aaguccaacc ggaagaccga cgggcgcgac aacgagcugg gcaugaucgc caccgggauc      480

gccgcgcucg acgcccagcu gaacggccgc acccugggcc aguaccucca cgagaugcgc      540

ggggccgacc cccggcagcg gggccaggug cgcguccggc cccaccugga gaucaacgac      600

aaggggcgcg agaucgccgc cuacggcuuc uacccccagc gggcgaugcu ggagcgcgag      660

uucaacgcca ucugggccgc ccaggcgccc caccacccgg agcuccugag cgaggagcgg      720

cgcgcccacc uguuccaggu gaucuucuac cagcggcccc ucaagcagcc cgaccccggg      780

cgcugcuccu ucaacccggc cgagccccgg cuggccaagg cccacccccu guuccagcgc      840

uuccggcucg ugaaggagau caacgagcug cgcaucguca cgggcagcaa cucccagggc      900

gugccccuga cccucgagca gcgggacauc cugcugaccc uccugcgcga caagaaggcg      960

agcuccuuca agacccugcu cgccaagauc aucaaggggc agccggaggc ccgguucaac     1020

aaggccagcg acucccgcga caagaugaag ggcgacgagc uggccgcgac gaugagcgac     1080

aagacccgcu ucgggcaggc cugguucgcc augcccgagg ccgagcagug gcggaucauc     1140

gccaagcugc gcgacaucga cgacccccuc gagcugaacg cguggcugga guccgaguac     1200

ggccucgccc ccgagcggcg cgacgccgug gccaacgccc ugcugccgca gggguacggc     1260

cggcucggcg agaccgcgcu gcgcaccaug cuggacuacc ucgaggccgc cgucaucagc     1320

gaggccgagg cggccgccca ggccggguac gaccacgccc uggcgcggcg cggcgccggg     1380

cuggaccagc ugcccaagua ccaggaggug cucggccggc gcaucccgcc cgggagcggc     1440

gagcccaacg accccuacga cauccggaug ggccgcaucu ccaaccccac ggugcacauc     1500

gcccugaacc agcugcggcg cgucgugaac gcccucaucc ggaaguacgg gaagcccgcc     1560

gagaucgcga ucgagcuggc ccgcgaccug aagcucagcg aggagcagaa gcgggaggug     1620

aacgccacca ucgccaagaa cacccgcgac gccgagcggc gcgcgguccg gcuggccgac     1680

gagggcaucg acaacaccgg gcacaaccgc gcccugcucc gcuucuggga ggagcuggcc     1740

gcggacccgc uggaccgggu gugcguguac ugcggcggcg ggaucggcgc cgccucccuc     1800

uucaacggga gcgucgacau cgaccacauc cugcccuggu cccgcacgcu ggacgacagc     1860

cgggccaacc gcauccucgu gcacaacucc ugcaaccggc ugaagcgcaa cuucgccccc     1920

uucgaggugc cggaguggcg ggagaucuac caggacaucc ugguccgcgc ggccaagcuc     1980

cccgccaaca agagcuggcg guucgccccc gacgccaugg accgcuucga gggcgagaag     2040

gacuuccugg cgcggcagcu gaccgacacc caguaccucg cccgcauggc ccacgaguac     2100

cuggccgccc uguaccccga cgaggagccg gacgaguggg gcguguucgc gcgggggaac     2160

cacguccgcg uggugcccgg ccggcucacc gagaugcugc gccgggccug ggggcugaac     2220

gccauccucu ccgacgacaa ccgcaagaac cggaacgacc accgccacca cgccaucgac     2280

gccgucgugg ugggcgucac gacccgcgac augcugaaca agaucagcca ggcggccggg     2340

cggggcgccg acaugggggc cgcggacgcc cugcgccggu ucgacccgcc cuggcccucc     2400

uuccaccagg ccgugcgcgc cgccguggag cggauccucg ucagccaccg cgcggaccac     2460

ggcacccugc ccggcaccga cgggcagggc uccacggccg ggcagcugca caacgacacc     2520

gccuacggcc ucaccgggga gacccggaag ggccuggccg uggugguccg ccggaagccc     2580

uaccugagcc ucgagccccg cgacaucgag acgauccggg acccgcagcu gcgcgaccac     2640

cuggccaccg agaccuacgg ccucgcgggg gccgaguuca ccgccgcccu gaagcggcug     2700

cagcagacgg acaaguucua cgccggcauc cgccgggugc gcgugaccga ggcgcucucc     2760

gucaucccca uccgggacgc caccgggcac gccuacaagg ccuacaaggg cgacgcgaac     2820

uuccgcuacg acguguggga gcugcccaac gggaaguggg ugcccgaggu caucagcaug     2880

uucgacgccc acgaccgggg cugggugucc caggugcacg ccgccaaccc ggccgcgcgc     2940

cggguccuga gccuccgcca gaacgacaug guggccuacc agcaccccga gcagggccug     3000

guguacgggc gcgucgugaa guucggccag uccgggcaga ugaccuucgc cgcccaccac     3060

gagggcggca gccucaagag ccgggacgcc gaccgcgacg accccuucaa guacuucuac     3120

aaguccgcgg ggacgaugaa ggacaucggc cugcggcaga uccgcgugga cgagaucggg     3180

cggguccagg accccggccc gcaggaccgc gacagcaagc gggugcgcaa guccgaccgg     3240

uga                                                                   3243


<210>  1544
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1544
auggccgccu ucaagcccaa cccgaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggacgaga accccaucug ccugaucgac      120

cugggggugc gcguguucga gcgggccgag guccccaaga ccggcgacuc ccucgccaug      180

gcccgccggc uggcgcgcag cgugcggcgc cugacccggc gccgggccca ccgccuccug      240

cgggcccgcc ggcugcucaa gcgcgagggg gugcugcagg ccgcggacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc gggccgccgc ccuggaccgc      360

aagcucacgc cccuggagug gagcgccguc cugcuccacc ugaucaagca ccggggcuac      420

cugucccagc gcaagaacga gggggagacc gcggacaagg agcucggcgc ccugcugaag      480

gggguggccg acaacgccca cgcccuccag accggcgacu uccggacccc cgcgaagcug      540

gcccugaaca aguucgagaa ggagagcggg cacauccgca accagcgggg cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaacc uccuguucga gaagcagaag      660

gaguucggca acccccacau cuccgacggg cugaaggagg gcaucgagac ccugcucaug      720

acccagcgcc cggcccugag cggggacgcc gugcagaaga ugcugggcca cugcacguuc      780

gagcccaccg agcccaaggc ggccaagaac accuacaccg ccgagcgguu cgucuggcuc      840

acgaagcuga acaaccugcg cauccucgag cagggcuccg agcagccccu gaccgacacc      900

cagcgggcca agcugaugga cgagccguac cgcaagagca agcucaccua cgcccaggcg      960

cggaagcugc uggggcucga ggacacggcc uucuucaagg gccugcgcua cgggaaggac     1020

aacgccgagg ccuccacccu gauggagaug aaggcguacc acgccaucag ccgggcccuc     1080

gagaaggagg gccugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggga ccgccuucuc ccuguucaag accgacgagg acaucacggg ccgccugaag     1200

gaccgggugc agcccgagau ccucgaggcc cugcugaagc acaucagcuu cgacaaguuc     1260

gugcagaucu cccucaaggc gcugcgccgg aucguccccc ugauggagca gggcaagcgc     1320

uacaacgagg ccugcgccga gaucuacggg gaccacuacg gcaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgga accccguggu gcugcgcgcc     1440

cugagccagg cgcggaaggu caucaacggg guggugcgcc gguacggcag ccccgcccgc     1500

auccacaucg agaccgcccg ggaggucggg aaguccuuca aggaccgcaa ggagaucgag     1560

aagcgccagg aggagaaccg gaaggaccgc gagaaggccg ccgcgaaguu ccaggaguac     1620

uuccccaacu ucgugggcga gcccaagagc aaggacaucc ucaagcugcg gcuguacgag     1680

cagcagcacg gcaagugccu cuacuccggg aaggagauca accugggccg ccugaacgag     1740

aagggguacg uggagaucga ccacgcccuc ccguucagcc ggaccuggga cgacuccuuc     1800

aacaacaagg uccuggugcu gggcagcgag aaccagaaca aggggaacca gacgcccuac     1860

gaguacuuca acggcaagga caacucccgc gaguggcagg aguucaaggc ccggguggag     1920

accagccgcu ucccccgguc caagaagcag cgcauccucc ugcagaaguu cgacgaggac     1980

ggcuucaagg agcggaaccu gaacgacacc cgcuacguca accgguuccu cugccaguuc     2040

guggccgacc gcaugcggcu gaccgggaag ggcaagaagc gcgucuucgc gagcaacggg     2100

cagaucacga accugcuccg gggcuucugg ggccugcgca aggugcgggc cgagaacgac     2160

cgccaccacg cccuggacgc cguggucgug gccugcucca ccguggcgau gcagcagaag     2220

aucacccggu ucguccgcua caaggagaug aacgccuucg acgggaagac caucgacaag     2280

gagacgggcg aggugcucca ccagaagacc cacuuccccc agccguggga guucuucgcc     2340

caggagguga ugauccgcgu cuucgggaag cccgacggca agcccgaguu cgaggaggcc     2400

gacaccccgg agaagcugcg gacccugcuc gccgagaagc ugagcucccg ccccgaggcg     2460

gugcacgagu acgugacgcc ccuguucguc agccgggccc ccaaccgcaa gauguccggg     2520

cagggccaca uggagaccgu caagagcgcc aagcggcucg acgagggcgu guccgugcug     2580

cgcgugccgc ugacccagcu caagcugaag gaccuggaga agauggugaa ccgggagcgc     2640

gagcccaagc ucuacgaggc ccugaaggcc cggcuggagg cgcacaagga cgaccccgcc     2700

aaggccuucg ccgagcccuu cuacaaguac gacaaggcgg ggaaccgcac ccagcagguc     2760

aaggccgugc ggguggagca gguccagaag accggcgugu gggugcgcaa ccacaacggg     2820

aucgccgaca acgccacgau gguccgggug gacguguucg agaagggcga caaguacuac     2880

cucgucccga ucuacagcug gcaggucgcc aaggggaucc ugcccgaccg cgcgguggug     2940

gccuacgccg acgaggagga cuggaccgug aucgacgagu ccuuccgguu caaguucgug     3000

cuguacagca acgaccucau caagguccag cugaagaagg acagcuuccu gggcuacuuc     3060

uccggccucg accgcgccac cggggccauc agccugcggg agcacgaccu ggagaagucc     3120

aagggcaagg acgggaugca ccgcaucggc gugaagaccg cgcucagcuu ccagaaguac     3180

cagaucgacg agauggggaa ggagauccgg cccugccgcc ugaagaagcg gcccccggug     3240

cgcuga                                                                3246


<210>  1545
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1545
auggccgccu ucaagcccaa ccccaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggacgaga accccaucug ccugaucgac      120

cugggggugc gcguguucga gcgggccgag guccccaaga ccggcgacuc ccucgccgcc      180

gcgcgccggc uggcccgcag cgugcggcgc cugacccggc gccgggccca ccgccuccug      240

cgggcccgcc ggcugcucaa gcgcgagggg gugcugcagg cggccgacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc ggagcgccgc ccuggaccgc      360

aagcucacgc cccuggagug guccgccguc cugcuccacc ugaucaagca ccggggcuac      420

cugagccagc gcaagaacga gggggagacc gcggacaagg agcucggcgc ccugcugaag      480

gggguggccg acaacgccca cgcccuccag accggcgacu uccggacccc cgcggagcug      540

gcccugaaca aguucgagaa ggaguccggg cacauccgca accggcgcgg cgacuacagc      600

cacaccuucu cccgcaagga ccuccaggcc gagcuggacc uccuguucga gaagcagaag      660

gaguucggca acccccacau cagcgacgac cugaaggagg ggaucgagac ccugcucaug      720

acccagcggc cggcccuguc cggcgacgcc gugcagaaga ugcuggggca cugcacguuc      780

gagcccaccg agcccaaggc ggccaagaac accuacaccg ccgagcgcuu cgucuggcuc      840

acgaagcuga acaaccugcg gauccucgag cagggcagcg agcgcccccu gaccgacacc      900

gagcgggcca cccugaugga cgagccguac cgcaagucca agcucacgua cgcccaggcg      960

cggaagcugc uggagcucga cgacaccgcc uucuucaagg gccugcgcua cgggaaggac     1020

aacgccgagg ccagcacccu gauggagaug aaggcguacc acgccaucuc ccgggcccuc     1080

gagaaggagg gccugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggga ccgccuucag ccuguucaag acggacgagg acaucaccgg ccgccugaag     1200

gaccgggugc agcccgagau ccucgaggug cugcugaagc acaucuccuu cgacaaguuc     1260

guccagauca gccucaaggc ccugcgccgg aucgugcccc ugauggagca ggggaagcgc     1320

uacgacgagg cgugcgccga gaucuacggc gaccacgacg gcaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgga accccguggu ccugcgcgcc     1440

cugagccagg cccggaaggu gaucaacgcg gugguccgcc gguacggguc ccccgcccgc     1500

auccacaucg agaccgcccg cgaggugggc aagagcuuca aggaccggaa ggagaucgag     1560

aagcgccagg aggagaaccg gaaggaccgc gagaaggccg ccgcgaaguu ccgggaguac     1620

uuccccaacu ucguggggga gcccaagucc aaggacaucc ucaagcugcg ccuguacgag     1680

cagcagcacg gcaagugccu cuacagcggg aaggagauca accugggccg gcugaacgag     1740

aagggcuacg ucgagaucga ccacgcccuc ccguucuccc gcacguggga cgacagcuuc     1800

aacaacaagg ugcuggugcu gggguccgag aaccagaaca agggcaacca gacccccuac     1860

gaguacuuca acgggaagga caacagccgg gaguggcagg aguucaaggc ccgcgucgag     1920

accucccggu ucccccgcag caagaagcag cggauccucc ugcagaaguu cgacgaggag     1980

ggcuucaagg agcgcaaccu gaacgacacc cgguacguga accgcuuccu cugccaguuc     2040

gucgccgacc acaugcugcu gacggggaag ggcaagcggc gcguguucgc guccaacggc     2100

cagaucacca accuccugcg gggguucugg ggccugcgca aggugcgggc cgagaacaac     2160

cgccaccacg cccucgacgc cgucguggug gccugcagca ccgucgcgau gcagcagaag     2220

aucacccgcu ucgugcggua caaggagaug aacgccuucg acgggaagac gaucgacaag     2280

gagaccggcg aggugcugca ccagaagacc cacuuccccc agccguggga guucuucgcc     2340

caggagguca ugauccgcgu guucggcaag cccgacggga agcccgaguu cgaggaggcc     2400

gacaccccgg agaagcugcg gacgcuccug gccgagaagc uguccagccg ccccgaggcg     2460

gugcacgagu acgucacgcc ccucuucgug ucccgggccc ccaaccgcaa gaugagcggc     2520

caggggcaca uggagaccgu gaaguccgcc aagcggcugg acgagggcgu cagcgugcug     2580

cgcgugccgc ucacccagcu gaagcugaag gaccucgaga agauggucaa ccgggagcgc     2640

gagcccaagc uguacgaggc ccugaaggcc cggcucgagg cgcacaagga cgaccccgcc     2700

aaggccuucg ccgagcccuu cuacaaguac gacaaggcgg ggaaccgcac ccagcagguc     2760

aaggccgugc ggguggagca gguccagaag accggcgugu gggugcgcaa ccacaacggc     2820

aucgccgaca acgccaccau ggugcgggug gacgucuucg agaaggccgg gaaguacuac     2880

cuggugccga ucuacuccug gcaggucgcg aagggcaucc ugcccgaccg cgccguggug     2940

gccuacgccg acgaggaggg guggaccgug aucgacgaga gcuuccgguu caaguucguc     3000

cucuacagca acgaccugau caaggugcag cugaagaagg acuccuuccu cggcuacuuc     3060

agcgggcugg accgcgccac cggcgcgauc ucccugcggg agcacgaccu cgagaagucc     3120

aagggcaagg acgggaugca ccgcaucggc gugaagacgg cccugagcuu ccagaaguac     3180

cagaucgacg agauggggaa ggagauccgc cugugccggc ucaagaagcg cccgcccguc     3240

cgguga                                                                3246


<210>  1546
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1546
augaccgccu ucaagcccaa ccccaucaac uacauccugg gccucgacau cgggaucgcc       60

agcgugggcu gggcgauggu cgagaucgac gaggaggaga accccauccg ccugaucgac      120

cugggggugc ggguguucga gcgcgccgag guccccaaga ccggcgacuc ccucgccgcc      180

gcccggcgcc uggcgcggag cgugcgccgg cugacgcgcc ggcgcgccca ccggcuccug      240

cgcgcccggc gccugcucaa gcgggagggg gugcugcagg ccgcggacuu cgacgagaac      300

ggccugauca agucccuccc caacaccccg uggcagcugc gcgccgccgc ccuggaccgg      360

aagcucacgc cccuggagug gagcgccguc cugcuccacc ugaucaagca ccgcggcuac      420

cugucccagc ggaagaacga gggggagacc gcggacaagg agcucggcgc ccugcugaag      480

gggguggccg acaacgccca cgcccuccag accggcgacu uccgcacccc cgcggagcug      540

gccgugaaca aguucgagaa ggagagcggg cacauccgga accagcgcgg cgacuacucc      600

cacaccuuca gccgcaagga ccugcaggcc gagcucgucc ugcuguucga gaagcagaag      660

gaguucggca acccccacau cuccgacggg cucaaggagg gcaucgagac ccugcugaug      720

gcccagcggc cggcccucag cggggacgcg gugcagaaga ugcugggcua cugcacguuc      780

gagcccaccg agcccaaggc cgccaagaac accuacaccg ccgagcgcuu caucuggcug      840

acgaagcuca acaaccugcg gauccuggag cagggcuccg agcgcccccu caccgacacc      900

gagcgggcca cccugaucga caugccguac aagaacaagc ugacguacgc gaaggcccgc      960

gagcuccugg ggcuggacga caccgccuuc uucaagggcc uccgguacgg gaaggacaac     1020

gccgagagcg cgacccugau ggagaugaag gccuaccacg ccauccgccg ggcccuggag     1080

aaggagggcc ucaaggacaa gaaguccccg cugagccugu cccccgagcu gcaggacgag     1140

aucgggaccg ccuucagccu cuucaagacg gacgaggaca ucaccggccg ccugaagggc     1200

ugccugcagc ccgagauccu cgacgcgcug cugaagcaca ucaacuucga caaguucgug     1260

cagaucuccc ucaaggcccu gcggcgcauc gucccccuga uggagcaggg gaagcgguac     1320

gacgaggccu gcgccgagac cuacggcgac cacuacgggg agaagaacag cgaggagaug     1380

cucuaccugc cgcccauccc cgccgacgag auccgcaacc ccguggugcu gcgggcgcuc     1440

ucccaggccc gcaaggucau caacggcgug gugcggcgcu acgggagccc cgcccggauc     1500

cacaucgaga ccgcccgcga ggucggcaag agcuucaagg accgcaagga gaucgagaag     1560

cggcaggagg agaaccgcaa ggaccgggag aaggccgcgg ccaaguuccg cgaguacuuc     1620

cccaacuucg ugggcgagcc caaguccaag gacauccuga agcugcggcu cuacgagcag     1680

cagcacggga agugccugua cagcggcaag gccaucgaca uccgccggcu gaacgggaag     1740

ggcuacgugg agaucgacca cgcccucccg uucucccgca cgugggacga cagcuucaac     1800

aacaaggucc uggugcuggg guccgagaac cagaacaagg gcaaccggac ccccuacgag     1860

uaccucaacg gcaaggacaa cagccgcgag uggcuggagu ucaaggcgcg gguggagacc     1920

ucccgcuucc cccacagcaa gaagcagcgg guccugcucc agaaguucga cgagaagggg     1980

uucaaggagc gcaaccugaa cgacacccgg uacgugaacc gcuuccugug ccaguucguc     2040

gccgaccaca ugcagcucac gggcaagggg aagcggcgcg uguucgccuc caacggccag     2100

aucaccaacc ugcugcgggg cuucuggggg cuccacaagg ugcgcgccga gaacgaccgg     2160

caccacgccc uggacgcggu cgugguggcc ugcagcaccg ucgccaugca gcagaagauc     2220

acgugcuucg ugcgcuacaa ggagaugaac gccuucgacg gcaagaccgu ggacaaggag     2280

accggggagg uccugcacca gaagacccac uucccccagc cgugggaguu cuucgcccag     2340

gaggugauga uccgcguguu cggcaagccc gacgggaagc ccgaguucga ggaggcggac     2400

accccggaga agcuccgggc ccugcuggcc gagaagcucu ccagccgccc cgaggccguc     2460

cacgaguacg ugacgccccu guucgugucc cgggccccca accgcaagau gagcggcgcg     2520

cacaaggaca cccugcgguc cgccaagcgc uucgucaagc acaacgagaa gaucagcgug     2580

aagcgggugu ggcucaccga gaucaagcug gccgaccugg agaacauggu caacuacaag     2640

aacggccgcg agaucgagcu cuacgaggcc cugaaggcgc ggcugggggc cuacggcggg     2700

aacgccaagc aggccuucga cccgaaggac aaccccuucu acaagaaggg cgggcagcuc     2760

gugaaggccg ugcgcgucga gaagacccag gaguccggcg ugcugcugaa caagaagaac     2820

gcguacacca ucgccgacaa cggcgacaug gugcgggucg acguguucug caagguggac     2880

aagaagggga agaaccagua cuucaucguc cccaucuacg ccuggcaggu cgccgagaac     2940

auccuccccg acaucgacug caagggcuac cgcaucgacg acagcuacac guucugcuuc     3000

ucccugcaca aguacgaccu gaucgccuuc cagaaggacg agaaguccaa gguggaguuc     3060

gcguacuaca ucaacugcga cagcagcaac gggcgguucu accucgccug gcacgacaag     3120

ggcagcaagg agcagcaguu ccgcaucucc acccagaacc uggugcugau gcagaaguac     3180

cagaucgacg agcucgggaa ggagauccgg ccgugccgcc ugaagaagcg gccgcccgug     3240

cgcuga                                                                3246


<210>  1547
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1547
auggccgccu ucaagcccaa ccccaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggacgaga accccaucug ccugaucgac      120

cugggggugc gcguguucga gcgggccgag guccccaaga ccggcgacuc ccucgccgcc      180

gcgcgccggc uggcccgcag cgugcggcgc cugacccggc gccgggccca ccgccuccug      240

cgggcccgcc ggcugcucaa gcgcgagggg gugcugcagg cggccgacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc ggagcgccgc ccuggaccgc      360

aagcucacgc cccuggagug guccgccguc cugcuccacc ugaucaagca ccggggcuac      420

cugagccagc gcaagaacga gggggagacc gcggacaagg agcucggcgc ccugcugaag      480

gggguggcca acaacgccca cgcccuccag accggcgacu uccggacccc cgcggagcug      540

gcccugaaca aguucgagaa ggaguccggg cacauccgca accagcgggg cgacuacagc      600

cacaccuucu cccgcaagga ccuccaggcc gagcugaucc uccuguucga gaagcagaag      660

gaguucggca acccccacgu gagcgggggc cugaaggagg ggaucgagac ccugcucaug      720

acccagcgcc cggcccuguc cggcgacgcc guccagaaga ugcugggcca cugcacguuc      780

gagcccgcgg agcccaaggc cgccaagaac accuacaccg ccgagcgguu caucuggcuc      840

accaagcuga acaaccugcg cauccucgag caggggagcg agcggccccu gacggacacc      900

gagcgcgcca cccugaugga cgagccguac cggaagucca agcucaccua cgcgcaggcc      960

cgcaagcugc ugggccucga ggacacggcc uucuucaagg ggcugcggua cggcaaggac     1020

aacgccgagg cgagcacccu gauggagaug aaggccuacc acgccaucuc ccgcgcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggca ccgccuucag ccuguucaag accgacgagg acaucacggg ccggcugaag     1200

gaccgcaucc agcccgagau ccucgaggcg cugcugaagc acaucuccuu cgacaaguuc     1260

gugcagauca gccucaaggc ccugcggcgc aucgugcccc ugauggagca ggggaagcgg     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgca accccgucgu gcugcgggcg     1440

cugagccagg cccgcaaggu gaucaacggc gucgugcggc gcuacggguc ccccgcccgg     1500

auccacaucg agaccgcccg cgaggugggc aagagcuuca aggaccgcaa ggagaucgag     1560

aagcggcagg aggagaaccg caaggaccgg gagaaggccg cggccaaguu ccgcgaguac     1620

uuccccaacu ucgucggcga gcccaagucc aaggacaucc ucaagcugcg gcuguacgag     1680

cagcagcacg ggaagugccu cuacagcggc aaggagauca accuggggcg ccugaacgag     1740

aagggcuacg uggagaucga ccacgcccuc ccguucuccc ggaccuggga cgacagcuuc     1800

aacaacaagg ugcugguccu gggguccgag aaccagaaca agggcaacca gacgcccuac     1860

gaguacuuca acggcaagga caacagccgc gaguggcagg aguucaaggc ccggguggag     1920

accucccgcu ucccccggag caagaagcag cgcauccucc ugcagaaguu cgacgaggac     1980

ggguucaagg agcggaaccu gaacgacacc cgcuacguga accgguuccu cugccaguuc     2040

gucgcggacc gcaugcggcu gaccggcaag gggaagaagc gcguguucgc cuccaacggc     2100

cagaucacga accugcuccg gggcuucugg gggcugcgca agguccgggc cgagaacgac     2160

cgccaccacg cccuggacgc cguggugguc gcgugcagca ccguggccau gcagcagaag     2220

aucacccgcu ucgugcggua caaggagaug aacgccuucg acggcaagac caucgacaag     2280

gagacggggg agguccucca ccagaagacc cacuuccccc agccguggga guucuucgcc     2340

caggagguga ugauccgcgu guucggcaag cccgacggga agcccgaguu cgaggaggcc     2400

gacaccccgg agaagcugcg gacccugcuc gcggagaagc uguccagccg ccccgaggcc     2460

guccacgagu acgugacgcc ccuguucgug ucccgggccc ccaaccgcaa gaugagcggc     2520

cagggccaca uggagaccgu caaguccgcc aagcggcucg acgagggggu gagcgugcug     2580

cgcgucccgc ugacccagcu caagcugaag gaccuggaga agauggugaa ccgggagcgc     2640

gagcccaagc ucuacgaggc ccugaaggcg cggcuggagg cccacaagga cgaccccgcc     2700

aaggccuucg cggagcccuu cuacaaguac gacaaggccg gcaaccgcac ccagcagguc     2760

aaggccgugc ggguggagca gguccagaag accggggugu gggugcgcaa ccacaacggc     2820

aucgccgaca acgccacgau ggugcggguc gacguguucg agaaggcggg gaaguacuac     2880

cucgugccga ucuacuccug gcaggucgcc aagggcaucc ugcccgaccg cgccguggug     2940

gccuacgccg acgaggaggg cuggaccguc aucgacgaga gcuuccgguu caaguucgug     3000

cuguacagca acgaccucau caaggugcag cugaagaagg acuccuuccu gggguacuuc     3060

agcggccucg accgcgcgac cggggccauc ucccugcggg agcacgaccu ggagaagucc     3120

aagggcaagg acgggaugca ccgcaucggc gucaagaccg cccucagcuu ccagaaguac     3180

cagaucgacg agaugggcaa ggagauccgc cugugccggc ugaagaagcg cccgcccgug     3240

cgguga                                                                3246


<210>  1548
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1548
augcugaagc gcgugcucgg caucgacguc gggaucagcu ccaucggcug ggcccugcug       60

gaguuccagg gggacaucga caacaaggac cuccccagcg gcaagauccu ggccuccggg      120

gugcgggugu ucacccaggc ggagaacccg aagaacggcg agagccuggc caagccccgc      180

cgggacgccc gccucgcccg gcgccggacc cgccggcgcg cccagcggcu gacggacauc      240

aaggaccugu ucuucaagua caagcucguc accggcaucu cccgcaacaa gaucaacagc      300

aucuacaaga ccgaggagaa caagaaggac cccuggaccc ugcggaagga ggcgcuguac      360

cgcaagcucg acggggagga guucgcccgg gugcugacgc acaucgccaa gcaccgcggc      420

uucaagucca ugcgcaagag cgaggaggag ggguccgaca ccaaggccaa ggagacccag      480

cgggugcuga ccgagaucgg caagaacaag gacacgcuca agaccgggau cuggcagacc      540

gcgggcgaga ugaucgccag ccggcccaag ggcgacgccc gccggaacaa gaccgacucc      600

uacaagaaca gcaucucccg cgagcugcug caggacgaga cgaagaucau cuucgagaag      660

cagcgcggga ugaacagcca guucaccucc gccgaccucg agaaggagua cagcgacauc      720

gccuuccacc aggcgccgau ccaguccaug cugaagaugg ucggcgggug caccuucgag      780

aaggacgagg agggcaagcg gcugaagcgc gcccccaaga acagcuacuc cgccgaggug      840

uucaccgccc ucacgaagau caacaaccug gugcugaaga ccaagaccgc caacaucaac      900

gaccgguucc ucaccacgga ggagcgcaag aaggcgcugg agcugugcca cagcaacgcc      960

aaggucaccu ucaagcagcu ccggggcgag cuggggcugc ccgaggacug gaucuucaac     1020

accguggacu acgccaagaa gcccaagaag accgccguga aggagacgga gccggagagc     1080

gccugcuucu uccagaugcg cggcuacaac gagauccgga aguccgucgg gaaggaccuc     1140

uggaccgaga ucagcaugaa gaaggagauc cugaacucca ucgugaccac ccuggcgacg     1200

gagaaguccg acgaggccau caaggcccag cucaagaagu ccgaggugcc cgacgagauc     1260

gccgagaagg uccugggccu gaacuuccgc ggguuccucc accugagcau caaggcccug     1320

gacaagauca uccccuucau gauggcgggc gacaaguaca ccgacgccug cgacaaggcc     1380

ggcuacaagg agcagaucag cgggaucaag aagaucuucc uccggaagcu gaacgacgag     1440

gagaacgagc agaucaccaa ccccguggcc aagcgcgcga ucacccaguu ccggaaggug     1500

aucaacgcca ucauccgcga guacggcaag uucgaccaga ugaacaucga gcuggcccgg     1560

gaccucgcca agagcuacga cgaccgcaag aagaucaagg aggggcagga ggacuuccgg     1620

gccgagaagg accgcgcggc ccagcggugc aaggacaacg gccuggaccc gaacuccaag     1680

gacaaccuga gcaaguaccg ccucugggag cagcaggaca acggguucug caucuacugc     1740

ggcaagccca ucgagcugca gcggcuggcc gagcagggcu acugcgaggu cgaccacauc     1800

cuccccuacu cccgcagccu ggacgacucc cagcacaaca aggugcuggc ccacauccac     1860

gacaaccagc agaagaagaa ccagaucccc uucgagcacu ucgggggcga cgggccgaag     1920

ugggaggagu ucgugggccg ccugaacagc caguucaaga accuccccaa guccaagcug     1980

aaccggcugg ucaagcgcaa guucgacgag aaggacaugg agggguucaa ggagcggaac     2040

aucaacgaca cgcgcuacau cagccgguuc aucaagaacu ucguggagaa ccaccucgag     2100

uucgccgagu ccgcgcagaa gcagaaggug uucguccgca gcggcucccu gaccgccuuc     2160

cugcggcacc aguggggccu caucaagaag cgcgacgaga gcgaccggca ccacgcccug     2220

gacgcccugg ugaccgccug cgcgacccag gccaugguca agagcacggc caucaccgcc     2280

cagaagaugg aguccuacaa cuggaucaac aaggagaagc cccgcaugcc ccucccgugg     2340

cugaacuucc gggacgacgu gcagaagucc cuggacgcgg uguucgucag ccgcgcgccc     2400

cggcacaagg ucgggggcgc cgcccacaag gagaccaucc gcagcauccg gaagugcggg     2460

gaggagaacc ucaucaucga gaagacgccc cugguggaca ucgccaccaa guccggcauc     2520

aagcugaagg acaucgagaa gcucuacaag aaggagcaga accgcaagcu guacgagacc     2580

cugaaggagc ggcucgagca gaacggcaac gaccccguga aggccuucgg gaacccggac     2640

gaccccgugc gcaugcccac caacgaccug accaagaccg gccccaucau caagagcauc     2700

aagcugauca cgaacaagaa guccgggcuc aacgugcggg ccggcaucgc cgacaacggg     2760

gagaucgccc gccuggacgu cuacgagcgg aagggcaagu ucuaccugau cccgaacuac     2820

guggccgacu ucgcgaacaa ggagcucccc aagaaggcca ucaaggccaa cacccccgag     2880

accgaguggc cgaucaucga ccgcaacuac aacuucaagu ucagccuguu caaggacgac     2940

cugaucucca ucaagcagaa gcucgacagc cccgaggagu ucuacuacua caagggcccc     3000

gacgugucca ccgccagcau cacggucgag uccguggacc gcaagcggcu gugggaggag     3060

aagaagaagg ggaagaucga gaacguguuc cacacccgca ucggcaccaa gacccucaag     3120

agcuuccgga aguucaaccu ggacauccug gggacgaagg cccccgucga gaucaagaag     3180

gagaccuggg ugggcgugau ccgcaagaac ggggucgcga acaacaccgg caacaaggcc     3240

auguga                                                                3246


<210>  1549
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1549
auggccgccu ucaagcccaa cccgaugaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac aaggaggaga accccauccg ccugaucgac      120

cugggggugc ggguguucga gcgcgccgag guccccaaga ccggcgacuc ccucgccaug      180

gcccggcgcc uggcgcggag cgugcgccgg cugacccgcc ggcgcgccca ccggcuccug      240

cgcgcccggc gccugcucaa gcgggagggg gugcugcagg acgccgacuu cgacgagaac      300

ggccugguca agucccuccc caacacgccg uggcagcugc gcgcggccgc ccuggaccgg      360

aagcucaccu gccuggagug gagcgccgug cugcuccacc ugaucaagca ccgcggcuac      420

cugucccagc ggaagaacga gggggagacc gccgacaagg agcucggcgc gcugcugaag      480

gggguggccg acaacgccca cgcccuccag accggcgacu uccgcacgcc cgccgagcug      540

gcgcugaaca aguucgagaa ggagagcggg cacauccgga accagcgcgg cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaacc ugcucuucga gaagcagaag      660

gaguucucca acccccacgu cagcgacucc cugaaggagg gcaucgagac ccugcucaug      720

gcccagcggc ccgcccugag cggggacgcc gugcagaaga ugcugggcca cugcaccuuc      780

gagccggcgg agcccaaggc cgccaagaac acguacaccg ccgagcgcuu caucuggcuc      840

accaagcuga acaaccugcg gauccucgag cagggguccg agcgcccccu gaccgacacg      900

gagcgggcca cccugaugga cgagcccuac cgcaagagca agcucaccua cgcgcaggcc      960

cggaagcugc ugggccucga ggacaccgcc uucuucaagg gccugcgcua cgggaaggac     1020

aacgccgagg cgccgacgcu gauggagaug aaggccuacc acgccaucuc ccgggcccuc     1080

gagaaggagg gccugaagga caagaagagc ccccugaacc uguccaccga gcuccaggac     1140

gagaucggga ccgccuucag ccuguucaag accgacaagg acaucacggg ccgccugaag     1200

gaccgggugc agcccgagau ccucgaggcg cugcugaagc acaucuccuu cgacaaguuc     1260

guccagauca gccucaaggc ccugcgccgg aucgugcccc ugauggagca ggggaagcgc     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacu gcaagaagaa cgccgaggag     1380

aagaucuacc ucccgcccau ccccgcggac gagauccgga accccguggu ccugcgcgcc     1440

cugagccagg cccggaaggu gaucaacugc gugguccgcc gguacggcuc cccggcccgc     1500

auccacaucg agaccgcccg cgaggugggg aagagcuuca aggaccggaa ggagaucgag     1560

aagcgccagg aggagaaccg gaaggaccgc gagaaggcgg ccgccaaguu ccgggaguac     1620

uuccccaacu ucgugggcga gcccaagucc aaggacaucc ucaagcugcg ccuguacgag     1680

cagcagcacg ggaagugccu cuacagcggc aaggagauca accugguccg gcugaacgag     1740

aagggguacg uggagaucga ccacgcccuc cccuucuccc gcaccuggga cgacagcuuc     1800

aacaacaagg ugcugguccu gggcuccgag aaccagaaca agggcaacca gaccccguac     1860

gaguacuuca acgggaagga caacagccgg gaguggcagg aguucaaggc gcgcguggag     1920

acgucccggu ucccccgcag caagaagcag cggauccucc ugcagaaguu cgacgaggag     1980

ggcuucaagg agcgcaaccu gaacgacacc cgguacguca accgcuuccu cugccaguuc     2040

guggccgacc acauccugcu gaccgggaag ggcaagcggc gcguguucgc cuccaacggg     2100

cagaucacca accuccugcg gggcuucugg ggccugcgca agguccgggc cgagaacgac     2160

cgccaccacg cccucgacgc gguggugguc gccugcagca cgguggccau gcagcagaag     2220

aucacccgcu ucgugcggua caaggagaug aacgccuucg acgggaagac caucgacaag     2280

gagaccggcg agguccugca ccagaaggcc cacuuccccc agcccuggga guucuucgcg     2340

caggagguga ugauccgcgu guucgggaag ccggacggca agcccgaguu cgaggaggcc     2400

gacacgcccg agaagcugcg gacccuccug gccgagaagc uguccagccg cccggaggcc     2460

guccacgagu acgugacccc ccucuucgug ucccgggccc ccaaccgcaa gaugagcggc     2520

caggggcaca uggagaccgu caaguccgcg aagcggcugg acgagggcau cagcgugcug     2580

cgcgugcccc ucacgcagcu gaagcugaag gggcucgaga agauggucaa ccgggagcgc     2640

gagccgaagc uguacgacgc ccugaaggcc cagcucgaga cccacaagga caaccccgcc     2700

aaggcguucg ccgagcccuu cuacaaguac gacaaggccg gcaaccggac ccagcaggug     2760

aaggccgugc gcaucgagca gguccagaag accggggugu gggugcggaa ccacaacggc     2820

aucgccgaca acgcgaccau gguccgcgug gacguguucg agaagggcgg gaaguacuac     2880

cuggucccca ucuacuccug gcagguggcc aagggcaucc ugccggaccg ggccgugguc     2940

gccuucaagg acgaggagga cuggacggug auggacgaca gcuucgaguu ccgcuucgug     3000

cucuacgcca acgaccugau caagcugacc gcgaagaaga acgaguuccu cggguacuuc     3060

gucagccuga accgggccac cggcgccauc gacauccgca cccacgacac ggacuccacc     3120

aaggggaaga acggcaucuu ccagagcgug ggcgugaaga ccgcccuguc cuuccagaag     3180

aaccagaucg acgagcucgg gaaggagauc cggcccugcc gccugaagaa gcgccccccc     3240

guccgguga                                                             3249


<210>  1550
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1550
auggccgccu ucaagcccaa cccgaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggaggaga accccauccg ccugaucgac      120

cugggggugc ggguguucga gcgcgccgag guccccaaga ccggcgacuc ccucgccaug      180

gcccggcgcc uggcgcggag cgugcgccgg cugacccgcc ggcgcgccca ccggcuccug      240

cgcgcccggc gccugcucaa gcgggagggg gugcugcagg ccgcggacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc gcgccgccgc ccuggaccgg      360

aagcucaccc cccuggagug gagcgccguc cugcuccacc ugaucaagca ccgcggcuac      420

cugucccagc ggaagaacga gggggagacc gcggacaagg agcucggcgc ccugcugaag      480

gggguggcca acaacgccca cgcccuccag accggcgacu uccgcacgcc cgcggagcug      540

gcccugaaca aguucgagaa ggagagcggg cacauccgga accagcgcgg cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaucc ugcucuucga gaagcagaag      660

gaguucggca acccccacgu guccgggggc cugaaggagg ggaucgagac ccugcucaug      720

acccagcggc cggcccugag cggcgacgcc guccagaaga ugcugggcca cugcacguuc      780

gagcccgcgg agcccaaggc cgccaagaac accuacaccg ccgagcgcuu caucuggcuc      840

accaagcuga acaaccugcg gauccucgag cagggguccg agcgcccccu gacggacacc      900

gagcgggcca cccugaugga cgagccguac cgcaagagca agcucaccua cgcgcaggcc      960

cggaagcugc ugggccucga ggacacggcc uucuucaagg ggcugcgcua cggcaaggac     1020

aacgccgagg cguccacccu gauggagaug aaggccuacc acgccaucag ccgggcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccccugaacc ugagcuccga gcuccaggac     1140

gagaucggca ccgccuucag ccuguucaag accgacgagg acaucacggg ccgccugaag     1200

gaccgggugc agcccgagau ccucgaggcg cugcugaagc acaucuccuu cgacaaguuc     1260

gugcagauca gccucaaggc ccugcgccgg aucguccccc ugauggagca ggggaagcgc     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgga accccguggu gcugcgcgcg     1440

cugagccagg cccggaaggu caucaacggc guggugcgcc gguacggguc cccggcccgc     1500

auccacaucg agaccgcccg cgaggucggc aagagcuuca aggaccggaa ggagaucgag     1560

aagcgccagg aggagaaccg gaaggaccgc gagaaggccg cggccaaguu ccgggaguac     1620

uuccccaacu ucgugggcga gcccaagucc aaggacaucc ucaagcugcg ccuguacgag     1680

cagcagcacg ggaagugccu cuacagcggc aaggagauca accuggugcg gcugaacgag     1740

aagggguacg ucgagaucga ccacgcccuc cccuucuccc gcaccuggga cgacagcuuc     1800

aacaacaagg ugcuggugcu gggcuccgag aaccagaaca aggggaacca gacgccguac     1860

gaguacuuca acggcaagga caacagccgg gaguggcagg aguucaaggc ccgcgucgag     1920

accucccggu ucccccgcag caagaagcag cggauccucc ugcagaaguu cgacgaggac     1980

ggcuucaagg agugcaaccu gaacgacacc cgcuacguga accgguuccu cugccaguuc     2040

gucgcggacc acauccugcu gaccgggaag ggcaagcgcc ggguguucgc cuccaacggg     2100

cagaucacga accuccugcg cggcuucugg ggccugcgga aggugcgcgc cgagaacgac     2160

cggcaccacg cccucgacgc cgucguggug gcgugcagca ccgucgccau gcagcagaag     2220

aucacccgcu ucgugcgcua caaggagaug aacgccuucg acgggaagac caucgacaag     2280

gagacgggca aggugcugca ccagaagacc cacuuccccc agcccuggga guucuucgcc     2340

caggagguca ugauccgggu guucgggaag ccggacggca agcccgaguu cgaggaggcc     2400

gacacccccg agaagcugcg cacccuccug gcggagaagc uguccagccg gccggaggcc     2460

gugcacgagu acgucacgcc ccucuucgug ucccgcgccc ccaaccggaa gaugagcggg     2520

cagggccaca uggagaccgu gaaguccgcc aagcgccugg acgagggcgu cagcgugcug     2580

cgggugcccc ucacccagcu gaagcugaag gaccucgaga agauggucaa ccgcgagcgg     2640

gagccgaagc uguacgaggc ccugaaggcg cgccucgagg cccacaagga cgaccccgcc     2700

aaggccuucg cggagcccuu cuacaaguac gacaaggccg ggaaccggac ccagcaggug     2760

aaggccgugc gcgucgagca ggugcagaag accggcgugu ggguccggaa ccacaacggg     2820

aucgccgaca acgccacgau ggugcgcgug gacgucuucg agaagggcga caaguacuac     2880

cuggugccca ucuacuccug gcagguggcg aaggggaucc ugccggaccg ggccgucgug     2940

cagggcaagg acgaggagga cuggcagcuc aucgacgaca gcuucaacuu caaguucagc     3000

cugcacccca acgaccuggu ggaggucauc accaagaagg cccgcauguu cggcuacuuc     3060

gccuccugcc accgggggac cggcaacauc aacauccgca uccacgaccu cgaccacaag     3120

aucgggaaga acggcauccu ggaggggauc ggcgugaaga ccgcccugag cuuccagaag     3180

uaccagaucg acgagcucgg caaggagauc cggcccugcc gccugaagaa gcgccccccg     3240

gugcgguga                                                             3249


<210>  1551
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1551
auggccgccu ucaagcccaa cccgaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggacgaga accccaucug ccugaucgac      120

cugggggugc gcguguucga gcgggccgag guccccaaga ccggcgacuc ccucgccaug      180

gcccgccggc uggcgcgcag cgugcggcgc cugacccggc gccgggccca ccgccuccug      240

cgggcccgcc ggcugcucaa gcgcgagggg gugcugcagg ccgcggacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc gggccgccgc ccuggaccgc      360

aagcucacgc cccuggagug gagcgccguc cugcuccacc ugaucaagca ccggggcuac      420

cugucccagc gcaagaacga gggggagacc gcggacaagg agcucggcgc ccugcugaag      480

gggguggccg acaacgccca cgcccuccag accggcgacu uccggacccc cgcggagcug      540

gcccugaaca aguucgagaa ggagagcggg cacauccgca accagcgggg cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaucc uccuguucga gaagcagaag      660

gaguucggca acccccacgu guccgggggc cugaaggagg ggaucgagac ccugcucaug      720

acccagcgcc cggcccugag cggcgacgcc guccagaaga ugcugggcca cugcacguuc      780

gagcccgcgg agcccaaggc cgccaagaac accuacaccg ccgagcgguu caucuggcuc      840

accaagcuga acaaccugcg cauccucgag cagggguccg agcggccccu gacggacacc      900

gagcgcgcca cccugaugga cgagccguac cgcaagagca agcucaccua cgcgcaggcc      960

cggaagcugc ugggccucga ggacacggcc uucuucaagg ggcugcggua cggcaaggac     1020

aacgccgagg cguccacccu gauggagaug aaggccuacc acgccaucag ccgcgcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggca ccgccuucuc ccuguucaag accgacgagg acaucacggg ccggcugaag     1200

gaccgcaucc agcccgagau ccucgaggcg cugcugaagc acaucagcuu cgacaaguuc     1260

gugcagaucu cccucaaggc ccugcggcgc aucgugcccc ugauggagca ggggaagcgg     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgca accccgucgu gcugcgggcg     1440

cugagccagg cccgcaaggu gaucaacggc gucgugcggc gcuacgggag ccccgcccgg     1500

auccacaucg agaccgcccg cgaggugggc aaguccuuca aggaccgcaa ggagaucgag     1560

aagcggcagg aggagaaccg caaggaccgg gagaaggccg cggccaaguu ccgcgaguac     1620

uuccccaacu ucgucggcga gcccaagagc aaggacaucc ucaagcugcg gcuguacgag     1680

cagcagcacg ggaagugccu cuacuccggc aaggagauca accuggggcg ccugaacgag     1740

aagggcuacg uggagaucga ccacgcccuc ccguucagcc ggaccuggga cgacuccuuc     1800

aacaacaagg ugcugguccu ggggagcgag aaccagaaca agggcaacca gacgcccuac     1860

gaguacuuca acggcaagga caacucccgc gaguggcagg aguucaaggc ccggguggag     1920

accagccgcu ucccccgguc caagaagcag cgcauccucc ugcagaaguu cgacgaggac     1980

ggguucaagg agcggaaccu gaacgacacc cgcuacguga accgguuccu cugccaguuc     2040

gucgcggacc gcaugcggcu gaccggcaag gggaagaagc gcguguucgc cagcaacggc     2100

cagaucacga accugcuccg gggcuucugg gggcugcgca agguccgggc cgagaacgac     2160

cgccaccacg cccuggacgc cguggugguc gcgugcucca ccguggccau gcagcagaag     2220

aucacccgcu ucgugcggua caaggagaug aacgccuucg acggcaagac caucgacaag     2280

gagacggggg agguccucca ccagaagacc cacuuccccc agccguggga guucuucgcc     2340

caggagguga ugauccgcgu guucggcaag cccgacggga agcccgaguu cgaggaggcc     2400

gacaccccgg agaagcugcg gacccugcuc gcggagaagc ugagcucccg ccccgaggcc     2460

guccacgagu acgugacgcc ccuguucgug agccgggccc ccaaccgcaa gauguccggc     2520

cagggccaca uggagaccgu caagagcgcc aagcggcucg acgagggggu guccgugcug     2580

cgcgucccgc ugacccagcu caagcugaag gaccuggaga agauggugaa ccgggagcgc     2640

gagcccaagc ucuacgaggc ccugaaggcg cggcuggagg cccacaagga cgaccccgcc     2700

aaggccuucg cggagcccuu cuacaaguac gacaaggccg gcaaccgcac ccagcagguc     2760

aaggccgugc ggguggagca gguccagaag accggggugu gggugcgcaa ccacaacggc     2820

aucgccgaca acgccacgau ggugcggguc gacguguucg agaaggggga caaguacuac     2880

cucgugccga ucuacagcug gcaggucgcg aagggcaucc ugcccgaccg cgccguggug     2940

cagggcaagg acgaggagga cuggcagcug aucgacgacu ccuucaacuu caaguucagc     3000

cuccacccca acgaccuggu cgaggugauc accaagaagg cccggauguu cggguacuuc     3060

gccagcugcc accgcggcac cgggaacauc aacauccgga uccacgaccu ggaccacaag     3120

aucggcaaga acgggauccu cgagggcauc ggcgugaaga ccgcccuguc cuuccagaag     3180

uaccagaucg acgagcuggg ggaggagauc cgccccugcc gccucaagaa gcggccgccc     3240

guccgcuga                                                             3249


<210>  1552
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1552
auggccgccu ucaagcccaa cccgaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggacgaga accccaucug ccugaucgac      120

cugggggugc gcguguucga gcgggccgag guccccaaga ccggcgacuc ccucgccaug      180

gcccgccggc uggcgcgcag cgugcggcgc cugacccggc gccgggccca ccgccuccug      240

cgggcccgcc ggcugcucaa gcgcgagggg gugcugcagg ccgcggacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc gggccgccgc ccuggaccgc      360

aagcucacgc cccuggagug gagcgccguc cugcuccacc ugaucaagca ccggggcuac      420

cugucccagc gcaagaacga gggggagacc gcggacaagg agcucggcgc ccugcugaag      480

gggguggccg acaacgccca cgcccuccag accggcgacu uccggacccc cgcggagcug      540

gcccugaaca aguucgagaa ggagagcggg cacauccgca accagcgggg cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaucc uccuguucga gaagcagaag      660

gaguucggca acccccacgu guccgggggc cugaaggagg ggaucgagac ccugcucaug      720

acccagcgcc cggcccugag cggcgacgcc guccagaaga ugcugggcca cugcacguuc      780

gagcccgcgg agcccaaggc cgccaagaac accuacaccg ccgagcgguu caucuggcuc      840

accaagcuga acaaccugcg cauccucgag cagggguccg agcggccccu gacggacacc      900

gagcgcgcca cccugaugga cgagccguac cgcaagagca agcucaccua cgcgcaggcc      960

cggaagcugc ugggccucga ggacacggcc uucuucaagg ggcugcggua cggcaaggac     1020

aacgccgagg cguccacccu gauggagaug aaggccuacc acgccaucag ccgcgcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggca ccgccuucuc ccuguucaag accgacgagg acaucacggg ccggcugaag     1200

gaccgcaucc agcccgagau ccucgaggcg cugcugaagc acaucagcuu cgacaaguuc     1260

gugcagaucu cccucaaggc ccugcggcgc aucgugcccc ugauggagca ggggaagcgg     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgca accccgucgu gcugcgggcg     1440

cugagccagg cccgcaaggu gaucaacggc gucgugcggc gcuacgggag ccccgcccgg     1500

auccacaucg agaccgcccg cgaggugggc aaguccuuca aggaccgcaa ggagaucgag     1560

aagcggcagg aggagaaccg caaggaccgg gagaaggccg cggccaaguu ccgcgaguac     1620

uuccccaacu ucgucggcga gcccaagagc aaggacaucc ucaagcugcg gcuguacgag     1680

cagcagcacg ggaagugccu cuacuccggc aaggagauca accuggggcg ccugaacgag     1740

aagggcuacg uggagaucga ccacgcccuc ccguucagcc ggaccuggga cgacuccuuc     1800

aacaacaagg ugcugguccu ggggagcgag aaccagaaca agggcaacca gacgcccuac     1860

gaguacuuca acggcaagga caacucccgc gaguggcagg aguucaaggc ccggguggag     1920

accagccgcu ucccccgguc caagaagcag cgcauccucc ugcagaaguu cgacgaggac     1980

ggguucaagg agcggaaccu gaacgacacc cgcuacguga accgguuccu cugccaguuc     2040

gucgcggacc gcaugcggcu gaccggcaag gggaagaagc gcguguucgc cagcaacggc     2100

cagaucacga accugcuccg gggcuucugg gggcugcgca agguccgggc cgagaacgac     2160

cgccaccacg cccuggacgc cguggugguc gcgugcucca ccguggccau gcagcagaag     2220

aucacccgcu ucgugcggua caaggagaug aacgccuucg acggcaagac caucgacaag     2280

gagacggggg agguccucca ccagaagacc cacuuccccc agccguggga guucuucgcc     2340

caggagguga ugauccgcgu guucggcaag cccgacggga agcccgaguu cgaggaggcc     2400

gacaccccgg agaagcugcg gacccugcuc gcggagaagc ugagcucccg ccccgaggcc     2460

guccacgagu acgugacgcc ccuguucgug agccgggccc ccaaccgcaa gauguccggc     2520

cagggccaca uggagaccgu caagagcgcc aagcggcucg acgagggggu guccgugcug     2580

cgcgucccgc ugacccagcu caagcugaag gaccuggaga agauggugaa ccgggagcgc     2640

gagcccaagc ucuacgaggc ccugaaggcg cggcuggagg cccacaagga cgaccccgcc     2700

aaggccuucg cggagcccuu cuacaaguac gacaaggccg gcaaccgcac ccagcagguc     2760

aaggccgugc ggguggagca gguccagaag accggggugu gggugcgcaa ccacaacggc     2820

aucgccgaca acgccacgau ggugcggguc gacguguucg agaaggggga caaguacuac     2880

cucgugccga ucuacagcug gcaggucgcg aagggcaucc ugcccgaccg cgccguggug     2940

cagggcaagg acgaggagga cuggcagcug aucgacgacu ccuucaacuu caaguucagc     3000

cuccacccca acgaccuggu cgaggugauc accaagaagg cccggauguu cggguacuuc     3060

gccagcugcc accgcggcac cgggaacauc aacauccgga uccacgaccu ggaccacaag     3120

aucggcaaga acgggauccu cgagggcauc ggcgugaaga ccgcccuguc cuuccagaag     3180

uaccagaucg acgagcuggg gaaggagauc cgccccugcc gccucaagaa gcggccgccc     3240

guccgcuga                                                             3249


<210>  1553
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1553
auggccgccu ucaagcccaa cccgaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggacgaga accccaucug ccugaucgac      120

cugggggugc gcguguucga gcgggccgag guccccaaga ccggcgacuc ccucgccgcc      180

gcgcgccggc uggcccgcag cgugcggcgc cugacccggc gccgggccca ccgccuccug      240

cgggcccgcc ggcugcucaa gcgcgagggg gugcugcagg cggccgacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc gggccgccgc ccuggaccgc      360

aagcucacgc cccuggagug gagcgcgguc cugcuccacc ugaucaagca ccggggcuac      420

cugucccagc gcaagaacga gggggagacc gccgacaagg agcucggcgc ccugcugaag      480

gggguggccg acaacgccca cgcgcuccag accggcgacu uccggacccc cgccgagcug      540

gcccugaaca aguucgagaa ggagagcggg cacauccgca accagcgggg cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaucc uccuguucga gaagcagaag      660

gaguucggca acccccacgu guccgggggc cugaaggagg ggaucgagac ccugcucaug      720

acccagcgcc cggcccugag cggcgacgcg guccagaaga ugcugggcca cugcacguuc      780

gagcccgccg agcccaaggc cgccaagaac accuacaccg ccgagcgguu caucuggcuc      840

accaagcuga acaaccugcg cauccucgag cagggguccg agcggccccu gacggacacc      900

gagcgcgcga cccugaugga cgagccguac cgcaagagca agcucaccua cgcccaggcc      960

cggaagcugc ugggccucga ggacacggcc uucuucaagg ggcugcggua cggcaaggac     1020

aacgcggagg ccuccacccu gauggagaug aaggccuacc acgccaucag ccgcgcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggca ccgcguucuc ccuguucaag accgacgagg acaucacggg ccggcugaag     1200

gaccgcgugc agcccgagau ccucgaggcc cugcugaagc acaucagcuu cgacaaguuc     1260

gugcagaucu cccucaaggc ccugcggcgc aucguccccc ugauggagca ggggaagcgg     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgcggac gagauccgca accccguggu gcugcgggcc     1440

cugagccagg cccgcaaggu caucaacggc guggugcggc gcuacgggag ccccgcccgg     1500

auccacaucg agaccgcccg cgaggucggc aaguccuuca aggaccgcaa ggagaucgag     1560

aagcggcagg aggagaaccg caaggaccgg gagaaggcgg ccgccaaguu ccgcgaguac     1620

uuccccaacu ucgugggcga gcccaagagc aaggacaucc ucaagcugcg gcuguacgag     1680

cagcagcacg ggaagugccu cuacuccggc aaggagauca accuggggcg ccugaacgag     1740

aagggcuacg uggagaucga ccacgcccuc ccguucagcc ggaccuggga cgacuccuuc     1800

aacaacaagg uccuggugcu ggggagcgag aaccagaaca agggcaacca gacgcccuac     1860

gaguacuuca acggcaagga caacucccgc gaguggcagg aguucaaggc gcggguggag     1920

accagccgcu ucccccgguc caagaagcag cgcauccucc ugcagaaguu cgacgaggac     1980

ggguucaagg agcggaaccu gaacgacacc cgcuacguca accgguuccu cugccaguuc     2040

guggccgacc gcaugcggcu gaccggcaag gggaagaagc gcgucuucgc cagcaacggc     2100

cagaucacga accugcuccg gggcuucugg gggcugcgca aggugcgggc cgagaacgac     2160

cgccaccacg cccuggacgc gguggucgug gccugcucca ccguggccau gcagcagaag     2220

aucacccgcu ucguccggua caaggagaug aacgccuucg acggcaagac caucgacaag     2280

gagacggggg aggugcucca ccagaagacc cacuuccccc agccguggga guucuucgcc     2340

caggagguga ugauccgcgu cuucggcaag cccgacggga agcccgaguu cgaggaggcg     2400

gacaccccgg agaagcugcg gacccugcuc gccgagaagc ugagcucccg ccccgaggcc     2460

gugcacgagu acgugacgcc ccuguucguc agccgggccc ccaaccgcaa gauguccggc     2520

cagggccaca uggagaccgu caagagcgcc aagcggcucg acgagggggu guccgugcug     2580

cgcgugccgc ugacccagcu caagcugaag gaccuggaga agauggugaa ccgggagcgc     2640

gagcccaagc ucuacgaggc gcugaaggcc cggcuggagg cccacaagga cgaccccgcc     2700

aaggcguucg ccgagcccuu cuacaaguac gacaaggccg gcaaccgcac ccagcagguc     2760

aaggccgugc ggguggagca gguccagaag accggggugu gggugcgcaa ccacaacggc     2820

aucgccgaca acgcgacgau gguccgggug gacguguucg agaaggggga caaguacuac     2880

cucgucccga ucuacagcug gcaggucgcc aagggcaucc ugcccgaccg cgccguggug     2940

cagggcaagg acgaggagga cuggcagcug aucgacgacu ccuucaacuu caaguucagc     3000

cuccacccca acgaccuggu ggaggugauc accaagaagg cccggauguu cggguacuuc     3060

gccagcugcc accgcggcac cgggaacauc aacauccgga uccacgaccu ggaccacaag     3120

aucggcaaga acgggauccu cgagggcauc ggcgucaaga ccgcgcuguc cuuccagaag     3180

uaccagaucg acgagcuggg gaaggagauc cgccccugcc gccucaagaa gcggccgccc     3240

gugcgcuga                                                             3249


<210>  1554
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1554
auggccgccu ucaagcccaa cccgaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggaggaga accccauccg ccugaucgac      120

cugggggugc ggguguucga gcgcgccgag guccccaaga ccggcgacuc ccucgccaug      180

gcccggcgcc uggcgcggag cgugcgccgg cugacccgcc ggcgcgccca ccggcuccug      240

cgcacgcggc gccugcucaa gcgggagggg gugcugcagg ccgccaacuu cgacgagaac      300

ggccugauca agucccuccc caacacccug uggcagcugc gcgcggccgc ccucgaccgg      360

aagcugaccc cgcuggagug gagcgccguc cuccugcacc ugaucaagca ccgcggcuac      420

cucucccagc ggaagaacga gggggagacc gccgacaagg agcugggcgc gcugcucaag      480

gggguggccg gcaacgccca cgcccugcag acgggggacu uccgcacccc cgccgagcug      540

gcgcucaaca aguucgagaa ggagagcggc cacauccgga accagcgcgg cgacuacucc      600

cacaccuuca gccgcaagga ccugcaggcc gagcugaacc uccuguucga gaagcagaag      660

gaguucggga acccccacau cuccgacggc cugaaggagg ggaucgagac ccuccugaug      720

acgcagcggc ccgcccugag cggcgacgcc gugcagaaga ugcucggcca cugcaccuuc      780

gagccgaccg agcccaaggc cgcgaagaac accuacacga ccgagcgcuu caucuggcug      840

accaagcuga acaaccuccg gauccuggag cagggguccg agcgcccccu gaccgacacg      900

gagcgggcca cccucaugga cgagcccuac cgcaagagca agcugaccua cgcccaggcc      960

cggaagcugc ucgagcugga cgacaucgcc uucuucaagg gccugcgcua cgggaaggac     1020

aacgcggagg ccuccacccu cauggagaug aaggccuacc acgccaucag ccgggcgcug     1080

gagaaggagg gccugaagga caagaagucc ccgcugaacc ucagccccga gcugcaggac     1140

gagaucggga cggccuucuc ccuguucaag accgacgagg acaucaccgg ccgccucaag     1200

gaccggaucc agcccgagau ccuggaggcc cugcucaagc acaucaacuu cgacaaguuc     1260

guccagauca gccugaaggc ccugcgccgg aucgugcccc ucauggagca gggcaagcgc     1320

uacgacgagg ccugcgcgga gaucuacggg gaccacuacg gcaagaagaa caccgaggag     1380

aagaucgacc ugccgcccau ccccgccgac gagauccaca accccguggu ccugcgggcc     1440

cucucccagg cccgcaaggu gaucaacggg gugguccggc gcuacggcag cccggcccgg     1500

auccacaucg agacggcgcg cgaggugggc aagagcuuca aggaccgcaa ggagaucgag     1560

aagcggcagg aggagaaccg caaggaccgg gagaaggccg ccgccaaguu ccgcgaguac     1620

uuccccaacu ucguggggga gcccaagucc aaggacaucc ugaagcugcg gcucuacgag     1680

cagcagcacg gcaagugccu guacagcggg aaggagauca accugggccg ccucaacgag     1740

aagggguacg ucgagaucga ccacgcccug cccuucuccc ggaccuggga cgacagcuuc     1800

aacaacaagg ugcuggugcu cggcuccgag aaccagaaca aggggaacca gaccccguac     1860

gaguacuuca acggcaagga caacagccgc gaguggcagg aguucaaggc gcgggucgag     1920

accucccgcu ucccccggag caagaagcag cgcauccugc ugcagaaguu cgacgaggac     1980

ggcuucaagg agcggaaccu caacgacacg cgcuacguga accgguuccu gugccaguuc     2040

guggccgacc gcaugcggcu gaccgggaag ggcaagaagc gcgucuucgc cuccaacggg     2100

cagaucacca accuccugcg gggcuucugg ggccugcgca aggugcgggc cgagaacgac     2160

cgccaccacg cgcucgacgc cgucguggug gccugcagca ccgucgccau gcagcagaag     2220

aucacgcgcu ucgugcggua caaggagaug aacgccuucg acgggaagac caucgacaag     2280

gagaccggcg aggugcugca ccagaagacc cacuuccccc agcccuggga guucuucgcg     2340

caggagguca ugauccgcgu guucgggaag ccggacggca agcccgaguu cgaggaggcc     2400

gacacgcccg agaagcugcg gacccuccug accgagaagc uguccagccg cccggaggcc     2460

gugcacgagu acgucacgcc ccucuucgug ucccgggccc ccaaccgcaa gaugagcggg     2520

cagggccaca uggagaccgu gaaguccgcc aagcggcugg acgagggcau cagcguccug     2580

cgcgugcccc ucacccagcu gaagcugaag gaccucgaga agauggugaa ccgggagcgc     2640

gagccgaagc uguacgaggc gcugaaggcc cggcucgagg cccacaagga cgaccccgcc     2700

aaggccuucg cggagcccuu cuacaaguac gacaaggccg ggaaccgcac ccagcagguc     2760

aaggccaugc ggguggagca gguccagaag accggcgugu gggugcgcaa ccacaacggg     2820

aucgccgaca acgcgaccau ggugcggguc gacguguucg agaagggcga caaguacuac     2880

cuggugccca ucuacuccug gcaggucgcc aaggggaucc ugccggaccg cgccguggug     2940

cagggcaagg acgaggagga cuggcagcuc aucgacgaca gcuucaacuu caaguucucc     3000

cugcacccca acgaccuggu cgaggugauc acgaagaagg cccggauguu cggcuacuuc     3060

gccagcugcc accgcgggac cggcaacauc aacauccgga uccacgaccu cgaccacaag     3120

aucgggaaga acggcauccu ggaggggauc ggcgugaaga ccgcgcugag cuuccagaag     3180

uaccagaucg acgagcucgg caaggagauc cgccccugcc gccugaagaa gcggcccccg     3240

guccgcuga                                                             3249


<210>  1555
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1555
auggccgccu ucaagcccaa cccgaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggacgaga accccaucug ccugaucgac      120

cugggggugc gcguguucga gcgggccgag guccccaaga ccggcgacuc ccucgccaug      180

gcccgccggc uggcgcgcag cgugcggcgc cugacccggc gccgggccca ccgccuccug      240

cgggcccgcc ggcugcucaa gcgcgagggg gugcugcagg ccgcggacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc gggccgccgc ccuggaccgc      360

aagcucacgc cccuggagug gagcgccguc cugcuccacc ugaucaagca ccggggcuac      420

cugucccagc gcaagaacga gggggagacc gcggacaagg agcucggcgc ccugcugaag      480

gggguggccg acaacgccca cgcccuccag accggcgacu uccggacccc cgcggagcug      540

gcccugaaca aguucgagaa ggagagcggg cacauccgca accagcgggg cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaacc uccuguucga gaagcagaag      660

gaguucggca acccccacau cuccgacggg cugaaggagg gcaucgagac ccugcucaug      720

acccagcgcc cggcccugag cggggacgcc gugcagaaga ugcugggcca cugcacguuc      780

gagcccgcgg agcccaaggc cgccaagaac accuacaccg ccgagcgguu caucuggcuc      840

accaagcuga acaaccugcg cauccucgag cagggcuccg agcggccccu gacggacacc      900

gagcgcacca cccugaucga cgagccguac cgcaagagca agcucacgua cgcccaggcg      960

cggaagcugc uggagcucga caacaccgcc uucuucaagg ggcugcggua cggcaaggac     1020

aacgccgagg ccuccacccu gauggagaug aaggcguacc acgccaucag ccgcgcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggca ccgccuucuc ccuguucaag acggacgagg acaucaccgg gcggcugaag     1200

gaccgcgucc agcccgagau ccucgaggcc cugcugaagc acaucagcuu cgacaaguuc     1260

gugcagaucu cccucaaggc gcugcggcgc aucgugcccc ugauggagca gggcaagcgg     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucgacc ucccgcccau ccccgccgac gagauccgca accccgucgu gcugcgggcc     1440

cugagccagg cgcgcaaggu gaucaacggc gucgugcggc gcuacgggag ccccgcccgg     1500

auccacaucg agaccgcccg cgaggugggc aaguccuuca aggaccgcaa ggagaucgag     1560

aagcggcagg aggagaaccg caaggaccgg gagaaggccg ccacgaaguu ccgcgaguac     1620

uuccccaacu ucgucgggga gcccaagagc aaggacaucc ucaagcugcg gcuguacgag     1680

cagcagcacg gcaagugccu cuacuccggc aaggagauca accuggggcg ccugaacgag     1740

aagggcuacg uggagaucga ccacgcgcuc ccguucagcc ggaccuggga cgacuccuuc     1800

aacaacaagg ugcugguccu ggggagcgag aaccagaaca agggcaacca gacccccuac     1860

gaguacuuca acgggaagga caacucccgc gaguggcagg aguucaaggc ccggguggag     1920

accagccgcu ucccccgguc caagaagcag cgcauccucc ugcagaaguu cgacgaggag     1980

ggcuucaagg agcggaaccu gaacgacacg cgcuacguga accgguuccu cugccaguuc     2040

gucgccgacc acaugcugcu gaccggcaag gggaagcgcc ggguguucgc cagcaacggc     2100

cagaucacca accuccugcg cggguucugg ggccugcgga agguccgcgc ggagaacgac     2160

cggcaccacg cccucgacgc cguggugguc gccugcucca ccguggccau gcagcagaag     2220

aucacgcgcu ucgugcgcua caaggagaug aacgcguucg acggcaagac caucgacaag     2280

gagaccgggg agguccugca ccagaagacc cacuuccccc agccguggga guucuucgcc     2340

caggagguga ugauccgggu guucggcaag cccgacggga agcccgaguu cgaggaggcc     2400

gacacgccgg agaagcugcg cacccuccug gccgagaagc ugagcucccg gcccgaggcc     2460

guccacgagu acgugacgcc ccucuucgug agccgcgcgc ccaaccggaa gauguccggc     2520

caggggcaca uggagaccgu caagagcgcc aagcgccugg acgagggcgu guccgugcug     2580

cgggucccgc ucacccagcu gaagcugaag gaccucgaga agauggugaa ccgcgagcgg     2640

gagcccaagc uguacgaggc ccugaaggcc cgccucgagg cccacaagga cgaccccgcg     2700

aaggccuucg ccgagcccuu cuacaaguac gacaaggccg gcaaccggac ccagcagguc     2760

aaggcggugc gcguggagca gguccagaag accggggugu gggugcggaa ccacaacggc     2820

aucgccgaca acgccaccau ggugcgcguc gacguguucg agaaggggga caaguacuac     2880

cuggugccga ucuacagcug gcaggucgcc aagggcaucc ugcccgaccg ggccguggug     2940

caggggaagg acgaggagga cuggcagcuc aucgacgacu ccuucaacuu caaguucagc     3000

cugcacccca acgaccuggu cgaggugauc accaagaagg cgcgcauguu cggcuacuuc     3060

gccagcugcc accggggcac ggggaacauc aacauccgca uccacgaccu cgaccacaag     3120

aucggcaaga acgggauccu ggagggcauc ggggugaaga ccgcccuguc cuuccagaag     3180

uaccagaucg acgagcucgg caaggagauc cggcccugcc gccugaagaa gcgcccgccc     3240

guccgguga                                                             3249


<210>  1556
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1556
auggccgccu ucaagcccaa cccgaugaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccaucgu cgagaucgac gaggaggaga accccauccg ccugaucgac      120

cugggggugc gcguguucga gcgcgccgag guccccaaga ccggcgacuc ccucgccgcc      180

gcgcggcgcc uggcccggag cgugcgccgg cugacccgcc ggcgcgccca ccggcuccug      240

cgcgcccggc gccugcucaa gcgggagggg gugcugcagg cggccgacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc gcgccgccgc ccuggaccgg      360

aagcucacgc cccuggagug gagcgcgguc cugcuccacc ugaucaagca ccgcggcuac      420

cugucccagc ggaagaacga gggggagacc gccgacaagg agcucggcgc ccugcugaag      480

gggguggccg acaacaccca cgcccuccag accggcgacu uccgcacccc cgcggagcug      540

gcccugaaca aguucgagaa ggagagcggg cacauccgga accagcgcgg cgacuacucc      600

cacaccuuca accgcaagga ccuccaggcc gagcugaacc uccuguucga gaagcagaag      660

gaguucggca acccccacgu gagcgacggg cugaaggagg gcaucgagac ccugcucaug      720

acgcagcggc cggcccuguc cggggacgcc guccagaaga ugcugggcca cugcaccuuc      780

gagcccaccg agcccaaggc ggccaagaac accuacacgg ccgagcgcuu cguguggcuc      840

accaagcuga acaaccugcg gauccucgag cagggcagcg agcgcccccu gaccgacacc      900

gagcgggcca cgcugaugga cgagccguac cgcaagucca agcucaccua cgcccaggcg      960

cggaagcugc uggaccucga cgacaccgcc uucuucaagg ggcugcgcua cggcaaggac     1020

aacgccgagg ccagcacccu gauggagaug aaggcguacc acgccaucuc ccgggcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggca cggccuucag ccuguucaag accgacgagg acaucaccgg gcgccugaag     1200

gaccgggugc agcccgagau ccucgaggcc cugcugaagc acaucuccuu cgacaaguuc     1260

guccagauca gccucaaggc gcugcgccgg aucgugcccc ugauggagca gggcaaccgc     1320

uacgacgagg ccugcaccga gaucuacggc gaccacuacg ggaagaagaa cacggaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgga accccguggu ccugcgcgcc     1440

cugucccagg cccggaaggu gaucaacggc gugguccgcc gguacgggag ccccgcgcgc     1500

auccacaucg agaccgcccg cgaggugggc aagagcuuca aggaccggaa ggagaucgag     1560

aagcgccagg aggagaaccg gaaggaccgc gagaaguccg ccgccaaguu ccgggaguac     1620

uuccccaacu ucguggggga gcccaagagc aaggacaucc ucaagcugcg ccuguacgag     1680

cagcagcacg gcaagugccu cuacuccggc aaggagauca accuggggcg gcugaacgag     1740

aagggcuacg ucgagaucga ccacgcccuc ccguucagcc gcaccuggga cgacuccuuc     1800

aacaacaagg ugcuggcgcu ggggagcgag aaccagaaca agggcaacca gacccccuac     1860

gaguacuuca acgggaagga caacucccgg gaguggcagg aguucaaggc ccgcguggag     1920

acgagccggu ucccccgcuc caagaagcag cggauccucc ugcagaaguu cgacgaggac     1980

ggcuucaagg agcgcaaccu gaacgacacc cgguacauca accgcuuccu cugccaguuc     2040

gucgccgacc acaugcugcu gaccggcaag gggaagcggc gcguguucgc cagcaacggc     2100

cagaucacca accuccugcg gggguucugg ggccugcgca agguccgggc ggagaacgac     2160

cgccaccacg cccucgacgc cguggugguc gccugcucca cgaucgccau gcagcagaag     2220

aucacccgcu ucgugcggua caaggagaug aacgcguucg acggcaagac caucgacaag     2280

gagaccgggg aggugcugca ccagaaggcc cacuuccccc agccguggga guucuucgcc     2340

caggagguca ugauccgcgu guucggcaag cccgacggga agcccgaguu cgaggaggcc     2400

gacacgccgg agaagcugcg gacccuccug gccgagaagc ugagcucccg ccccgaggcg     2460

gugcacaagu acgucacgcc ccucuucauc agccgggccc ccaaccgcaa gauguccggc     2520

caggggcaca uggagaccgu caagagcgcc aagcggcugg acgagggcau cuccgugcug     2580

cgcgugccgc ucacccagcu gaagcugaag gaccucgaga agauggugaa ccgggagcgc     2640

gagcccaagc uguacgaggc ccugaaggcc cggcucgagg cgcacaagga cgaccccgcc     2700

aaggccuucg ccgagcccuu cuacaaguac gacaaggcgg gcaaccgcac ccagcagguc     2760

aaggccgugc ggguggagca gguccagaag accggggugu gggugcacaa ccacaacggc     2820

aucgccgaca acgccaccau cgugcgcguc gacguguucg agaagggcgg gaaguacuac     2880

cuggugccga ucuacagcug gcaggucgcc aaggggaucc ugcccgaccg ggcgguggug     2940

cagggcaagg acgaggagga cuggaccguc auggacgacu ccuucgaguu caaguucgug     3000

cucuacgcca acgaccugau caagcugacg gccaagaaga acgaguuccu cggcuacuuc     3060

gugagccuga accgcgccac cggggccauc gacauccgga cccacgacac cgacagcacg     3120

aagggcaaga acgggaucuu ccaguccguc ggcgugaaga ccgcgcugag cuuccagaag     3180

uaccagaucg acgagcucgg gaaggagauc cgccccugcc ggcugaagaa gcggcccccg     3240

gugcgcuga                                                             3249


<210>  1557
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1557
auggccgccu ucaagcccaa cagcaucaac uacauccugg gccucgacau cgggaucgcg       60

uccgugggcu gggccauggu cgagaucgac aaggaggaga accccauccg ccugaucgac      120

cugggggugc ggguguucga gcgcgccgag guccccaaga ccggcgacag ccucgccgcc      180

gcgcggcgcc uggcccgguc cgugcgccgg cugacccgcc ggcgcgccca ccggcuccug      240

cgcacgcggc gccugcucaa gcgggagggg gugcugcagg ccgcgaacuu cgacaagaac      300

ggccugauca agagccuccc caacacccug uggcagcugc gcgccgccgc ccucgaccgg      360

aagcugacgc cccuggagug guccgccguc cuccugcacc ugaucaagca ccgcggcuac      420

cucagccagc ggaagaacga gggggagacc gcggacaagg agcugggcgc ccugcucaag      480

gggguggccg gcaacgccca cgcccugcag accggggacu uccgcacccc ggcggagcug      540

gcccucaaca aguucgagaa ggaguccggc cacauccgga accagcgcgg cgacuacagc      600

cacaccuucu cccgcaagga ccugcaggcc gagcugaacc uccuguucga gaagcagaag      660

gaguucggga acccccacau cagcggcggg cugaaggagg gcaucgagac ccuccugaug      720

acgcagcggc ccgcccuguc cggcgacgcc gugcagaaga ugcucgggca cugcaccuuc      780

gagcccaccg agccgaaggc ggccaagaac accuacacga ccgagcgcuu caucuggcug      840

accaagcuga acaaccuccg gauccuggag cagggcagcg agcgcccccu gaccgacacg      900

gagcgggcca cccucaugga cgagcccuac cgcaagucca agcugaccua cgcccaggcc      960

cggaagcugc ucagccugga ggacaccgcg uucuucaagg ggcugcgcua cggcaaggac     1020

aacgccgagg ccuccacgcu cauggagaug aaggccuacc acaccaucag ccgggcgcug     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ucagccccga gcugcaggac     1140

gagaucggca ccgccuucuc ccuguucaag accgacgagg acaucacggg ccgccucaag     1200

gaccggaucc agcccgagau ccuggaggcc cugcucaagc acaucagcuu cgacaaguuc     1260

guccagauca gccugaaggc ccugcgccgg aucgugcccc ucauggagca ggggaagcgc     1320

uacgacgagg ccugcgcgga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucuacc ugcccccgau ccccgccgac gagauccgga accccguggu ccugcgcgcc     1440

cucucccagg cccggaaggu gaucaacggc gugguccgcc gguacgggag ccccgcccgc     1500

auccacaucg agaccgcgcg cgaggugggc aaguccuuca aggaccggaa ggagaucgag     1560

aagcgccagg aggagaaccg gaaggaccgc gagaaggccg ccgccaaguu ccgggaguac     1620

uucccgaacu ucgugggcga gcccaagagc aaggacaucc ugaagcugcg ccucuacgag     1680

cagcagcacg ggaagugccu guacuccggc aaggagauca accugguccg gcucaacgag     1740

aagggguacg uggagaucga ccacgcccug cccuucagcc gcaccuggga cgacuccuuc     1800

aacaacaagg ugcugguccu cggcagcgag aaccagaaca aggggaacca gacgcccuac     1860

gaguacuuca acggcaagga caacucccgg gaguggcagg aguucaaggc gcgcguggag     1920

accagccggu ucccgcgcuc caagaagcag cggauccugc ugcagaaguu cgacgaggac     1980

ggcuucaagg agcgcaaccu caacgacacc cgguacguga accgcuuccu gugccaguuc     2040

gucgccgacc acauccugcu caccgggaag ggcaagcggc gcguguucgc cagcaacggg     2100

cagaucacga accugcugcg gggcuucugg ggccuccgca agguccgggc cgagaacgac     2160

cgccaccacg cgcuggacgc cguggugguc gccugcucca ccguggccau gcagcagaag     2220

aucacccgcu ucgugcggua caaggagaug aacgccuucg acgggaagac caucgacaag     2280

gagacgggcg agguccugca ccagaagacc cacuuccccc agcccuggga guucuucgcg     2340

caggagguga ugauccgcgu guucgggaag cccgacggca agccggaguu cgaggaggcc     2400

gacacccucg agaagcugcg gacccugcuc gccgagaagc ugagcucccg ccccgaggcc     2460

guccacgagu acgugacgcc ccuguucgug agccgggccc cgaaccgcaa gauguccggg     2520

cagggccaca uggagaccgu caagagcgcg aagcggcucg acgagggcgu guccgugcug     2580

cgcguccccc ugacccagcu caagcugaag gaccuggaga agauggugaa ccgggagcgc     2640

gagcccaagc ucuacgaggc ccugaaggcc cggcuggagg cccacaagga cgaccccgcc     2700

aaggcguucg ccgagccguu cuacaaguac gacaaggccg ggaaccgcac ccagcagguc     2760

aaggccgugc ggguggagca gguccagaag accggcgugu gggugcgcaa ccacaacggg     2820

aucgcggaca acgccacgau ggugcggguc gacguguucg agaagggcga caaguacuac     2880

cucgugccca ucuacagcug gcaggucgcc aaggggaucc ugcccgaccg cgccguggug     2940

cagggcaagg acgaggagga cuggcagcug aucgacgaca gcuucaacuu caaguucucc     3000

cuccacccca acgaccuggu cgaggugauc accaagaagg cccggauguu cggcuacuuc     3060

gcgagcugcc accgcgggac cggcaacauc aacauccgga uccacgaccu ggaccacaag     3120

aucgggaaga acggcauccu cgaggggauc ggcgugaaga ccgcccuguc cuuccagaag     3180

uaccagaucg acgagcuggg caaggagauc cgcccgugcc gccucaagaa gcggccgccc     3240

guccgcuga                                                             3249


<210>  1558
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1558
auggccgccu ucaagcccaa cccgaucaac uacauccugg gccucgacau cgggaucgcg       60

agcgugggcu gggccauggu cgagaucgac gaggaggaga accccauccg ccugaucgac      120

cugggggugc ggguguucga gcgcgccgag guccccaaga ccggcgacuc ccucgccaug      180

gugcggcgcc uggcccggag cgugcgccgg cugacccgcc ggcgcgcgca ccggcuccug      240

cgcgcccggc gccugcucaa gcgggagggg guccugcagg ccgccgacuu cgacgagaac      300

ggccugauca agucccuccc caacacgccg uggcagcugc gcgcggccgc ccuggaccgg      360

aagcucacgc cccuggagug gagcgccgug cugcuccacc ugaucaagca ccgcggcuac      420

cugucccagc ggaagaacga gggggagacc gccgacaagg agcucggcgc gcugcugaag      480

gggguggccg acaacgccca cgcccuccag accggcgacu uccgcacccc cgccgagcug      540

gcgcugaaca aguucgagaa ggagagcggg cacauccgga accagcgcgg cgacuacucc      600

cacaccuuca gccgcaagga ccuccaggcc gagcugaucc uccuguucga gaagcagaag      660

gaguucggca acccccacau cuccgggggc cugaaggagg ggaucgagac ccugcucaug      720

acccagcggc cggcccugag cggcgacgcc guccagaaga ugcugggcca cugcacguuc      780

gagcccgccg agcccaaggc ggccaagaac accuacaccg ccgagcgcuu caucuggcuc      840

accaagcuga acaaccugcg gauccucgag cagggguccg agcgcccccu gacggacacc      900

gagcgggcca cccugaugga cgagccguac cgcaagagca agcucaccua cgcccaggcg      960

cggaagcugc ugggccucga ggacacggcc uucuucaagg ggcugcgcua cggcaaggac     1020

aacgccgagg ccuccacccu gauggagaug aaggcguacc acgccaucag ccgggcccuc     1080

gagaaggagg ggcugaagga caagaagucc ccgcugaacc ugagccccga gcuccaggac     1140

gagaucggca ccgccuucuc ccuguucaag accgacgagg acaucacggg ccgccugaag     1200

gaccggaucc agcccgagau ccucgaggcc cugcugaagc acaucagcuu cgacaaguuc     1260

gugcagaucu cccucaaggc gcugcgccgg aucgugcccc ugauggagca ggggaagcgc     1320

uacgacgagg ccugcgccga gaucuacggc gaccacuacg ggaagaagaa caccgaggag     1380

aagaucuacc ucccgcccau ccccgccgac gagauccgga accccgucgu gcugcgcgcc     1440

cugagccagg cgcggaaggu gaucaacggc gucgugcgcc gguacgggag ccccgcccgc     1500

auccacaucg agaccgcccg cgaggugggc aaguccuuca aggaccggaa ggagaucgag     1560

aagcgccagg aggagaaccg gaaggaccgc gagaaggccg ccgcgaaguu ccgggaguac     1620

uuccccaacu ucgucggcga gcccaagagc aaggacaucc ucaagcugcg ccuguacgag     1680

cagcagcacg ggaagugccu cuacuccggc aaggagauca accuggggcg gcugaacgag     1740

aagggcuacg uggagaucga ccacgcccuc ccguucagcc gcaccuggga cgacuccuuc     1800

aacaacaagg ugcugguccu ggggagcgag aaccagaaca agggcaacca gacgcccuac     1860

gaguacuuca acggcaagga caacucccgg gaguggcagg aguucaaggc ccgcguggag     1920

accagccggu ucccccgcuc caagaagcag cggauccucc ugcagaaguu cgacgaggac     1980

ggguucaagg agcgcaaccu gaacgacacc cgguacguga accgcuuccu cugccaguuc     2040

gucgccgacc ggaugcgccu gaccggcaag gggaagaagc ggguguucgc gagcaacggc     2100

cagaucacga accugcuccg cggcuucugg gggcugcgga agguccgcgc cgagaacgac     2160

cgccaccacg cccuggacgc cguggugguc gccugcucca ccguggcgau gcagcagaag     2220

aucacccggu ucgugcgcua caaggagaug aacgccuucg acggcaagac caucgacaag     2280

gagacggggg agguccucca ccagaagacc cacuuccccc agccguggga guucuucgcc     2340

caggagguga ugauccgggu guucggcaag cccgacggga agcccgaguu cgaggaggcc     2400

gacaccccgg agaagcugcg cacccugcuc gccgagaagc ugagcucccg gcccgaggcg     2460

guccacgagu acgugacgcc ccuguucgug agccgcgccc ccaaccggaa gauguccggc     2520

cagggccaca uggagaccgu caagagcgcc aagcgccucg acgagggggu guccgugcug     2580

cgggucccgc ugacccagcu caagcugaag gaccuggaga agauggugaa ccgcgagcgg     2640

gagcccaagc ucuacgaggc ccugaaggcc cgccuggagg cgcacaagga cgaccccgcc     2700

aaggccuucg ccgagcccuu cuacaaguac gacaaggcgg gcaaccggac ccagcagguc     2760

aaggccgugc gcguggagca gguccagaag accggggugu gggugcggaa ccacaacggc     2820

aucgccgaca acgccacgau ggugcgcguc gacguguucg agaaggggga caaguacuac     2880

cucgugccga ucuacagcug gcaggucgcc aagggcaucc ugcccgaccg ggcgguggug     2940

cagggcaagg acgaggagga cuggcagcug aucgacgacu ccuucaacuu caaguucagc     3000

cuccacccca acgaccuggu cgaggugauc accaagaagg cccgcauguu cggguacuuc     3060

gccagcugcc accggggcac cgggaacauc aacauccgca uccacgaccu ggaccacaag     3120

aucggcaaga acgggauccu cgagggcauc ggcgugaaga ccgcccuguc cuuccagaag     3180

uaccagaucg acgagcuggg gaaggagauc cgccccugcc ggcucaagaa gcgcccgccc     3240

guccgguga                                                             3249


<210>  1559
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1559
augcgcgugc ugggccucga cgccgggauc gccagccugg gcugggcgcu gaucgagauc       60

gaggagucca accgcgggga gcucagccag ggcaccauca ucggggccgg caccuggaug      120

uucgacgccc ccgaggagaa gacgcaggcc ggcgccaagc ugaaguccga gcagcggcgg      180

accuuccgcg ggcagcggcg cgucgugcgg cgccggcgcc agcggaugaa cgaggugcgc      240

cggauccugc acagccacgg ccuccugccg uccagcgacc gcgacgcgcu gaagcagccc      300

gggcucgacc ccuggcggau ccgcgccgag gcccuggacc ggcugcucgg ccccgucgag      360

cuggccgugg cgcuggggca caucgcccgc caccggggcu ucaaguccaa cagcaagggc      420

gccaagacca acgacccggc cgacgacacc uccaagauga agcgcgccgu gaacgagacg      480

cgggagaagc ucgcgcgcuu cgggagcgcc gccaagaugc uggucgagga cgaguccuuc      540

gugcugcgcc agacccccac caagaacggc gccagcgaga ucgugcggcg cuuccggaac      600

cgcgaggggg acuacucccg gagccuccug cgcgacgacc uggccgcgga gaugcgggcc      660

cucuucaccg cccaggcccg cuuccagucc gccaucgcga cggccgaccu gcagaccgcc      720

uucaccaagg ccgccuucuu ccagcggccc cugcaggaca gcgagaagcu cgucggcccc      780

ugcccguucg agguggacga gaagcgcgcg cccaagcggg gcuacuccuu cgagcuguuc      840

cgcuuccuga gccggcucaa ccacgugacc cugcgcgacg ggaagcagga gcggacgcug      900

acccgcgacg agcucgcccu ggccgccgcg gacuucggcg ccgccgccaa ggucuccuuc      960

accgcccugc ggaagaagcu caagcugccc gagaccacgg uguucguggg ggucaaggcg     1020

gacgaggaga gcaagcugga cgugguggcc cgcagcggca aggccgccga ggggaccgcc     1080

cggcuccgcu ccgucaucgu ggacgcgcug ggcgagcugg ccuggggcgc ccuccugugc     1140

agccccgaga agcuggacaa gaucgccgag gugaucuccu uccgcagcga caucgggcgg     1200

aucuccgagg gccucgccca ggcggggugc aacgccccgc uggucgacgc ccugaccgcc     1260

gcggccagcg acggccgcuu cgaccccuuc accggggccg gccacaucuc cagcaaggcc     1320

gcccggaaca uccucuccgg ccugcgccag gggaugacgu acgacaaggc gugcugcgcc     1380

gccgacuacg accacaccgc cagccgggag cgcggcgccu ucgacguggg cgggcacggg     1440

cgggaggcgc ugaagcgcau ccuccaggag gagcggaucu cccgcgagcu ggugggcagc     1500

cccaccgccc ggaaggcccu gaucgagucc aucaagcagg ucaaggccau cguggagcgc     1560

uacggcgucc ccgaccggau ccacguggag cuggcccgcg acgugggcaa gagcaucgag     1620

gagcgggagg agaucacccg cgggaucgag aagcggaacc gccagaagga caagcuccgg     1680

gggcuguucg agaaggaggu cggccgcccg ccccaggacg gcgcgcgggg gaaggaggag     1740

cugcuccgcu ucgagcugug guccgagcag augggccgcu gccuguacac ggacgacuac     1800

aucagccccu cccagcucgu ggccaccgac gacgccgugc aggucgacca cauccugccc     1860

uggagccggu ucgccgacga cuccuacgcg aacaagaccc ugugcauggc caaggccaac     1920

caggacaaga aggggcgcac cccguacgag ugguucaagg ccgagaagac ggacaccgag     1980

ugggacgccu ucaucgugcg gguggaggcg cucgccgaca ugaagggcuu caagaagcgc     2040

aacuacaagc ugcggaacgc cgaggaggcc gccgcgaagu uccgcaaccg gaaccugaac     2100

gacacccgcu gggccugccg gcuccuggcc gaggcccuga agcagcucua ccccaagggg     2160

gagaaggaca aggacggcaa ggagcgccgg cgcgucuuca gccggcccgg cgcccugacc     2220

gaccgccugc ggcgcgcgug ggggcuccag uggaugaaga aguccacgaa gggcgaccgg     2280

auccccgacg accgccacca cgcccuggac gccaucguga ucgccgccac caccgagagc     2340

cugcuccagc gggcgacccg cgaggugcag gagaucgagg acaaggggcu gcacuacgac     2400

cuggucaaga acgugacgcc gcccuggccc ggcuuccgcg agcaggccgu ggaggccguc     2460

gagaaggugu ucguggcccg ggcggagcgc cggcgcgccc gggggaaggc ccacgacgcc     2520

accauccgcc acaucgccgu ccgggagggc gagcagcgcg uguacgagcg gcgcaaggug     2580

gcggagcuca agcuggccga ccuggaccgg gucaaggacg ccgagcgcaa cgcccggcuc     2640

aucgagaagc ugcgcaacug gaucgaggcc ggcagcccca aggacgaccc gccccugucc     2700

cccaaggggg acccgaucuu caaggugcgg cucgugacca agagcaaggu caacaucgcg     2760

cuggacaccg gcaaccccaa gcgccccggg accguggacc ggggcgagau ggcccgcgug     2820

gacgucuucc ggaaggccuc caagaagggg aaguacgagu acuaccuggu gcccaucuac     2880

ccgcacgaca ucgccacgau gaagaccccg cccauccgcg ccgugcaggc guacaagccc     2940

gaggacgagu ggcccgagau ggacagcucc uacgaguucu gcuggagccu cguccccaug     3000

accuaccucc aggugaucuc cagcaagggc gagaucuucg agggcuacua ccgggggaug     3060

aaccgcuccg ugggcgccau ccagcugagc gcccacucca acagcuccga cgucgugcag     3120

gggaucggcg cccgcacccu gacggaguuc aagaaguuca acguggaccg guucgggcgc     3180

aagcacgagg ucgagcggga gcugcgcacc uggcggggcg agaccuggcg cggcaaggcg     3240

uacaucuga                                                             3249


<210>  1560
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1560
augaagaaca cccuguacgg caucgggcuc gacaucggcg uggccagcgu cgggugggcc       60

guggugggcc ugaacgggac cggcgagccc gucggccugc accgccucgg ggugcggauc      120

uucgacaagg cggagcagcc gaagacgggc gagucccugg ccgcgccccg ccggauggcc      180

cgcgggaugc ggcgccggcu gcgccggaag gcccuccgcc gggcggacgu guacgcccug      240

cuggagcgca gcggccucuc cacccgggag gcccuggccc acauguucga ggccgggggc      300

cuggaggaca ucuacgcccu ccgcacccgg gcccuggacg agcccgucgg caaggccgag      360

uucagccgca uccugcucca ccuggcccag cggcgcgggu ucaaguccaa ccggcgcacc      420

gcgagcgacg gcgaggacgg gcggcugcuc gccgccguga acgagaaccg ccgccggaug      480

gcccagggcg gcuggcgcac ggugggggag augcuguacc ggcacgaggc cuucgcgcug      540

cacaagcgca acaaggccga cgaguaccuc uccaccgucg gccgggacau gguggccgag      600

gaggccagcc ugcuguucca gcgccagcgg gagcucgggu gcgccugggc gacccccgag      660

cugcaggccg aguaccuguc cauccuccug cgccagcgga gcuucgacga gggccccggg      720

ggcaacuccc cguacggcgg gaaccaggug gagaagaugg ucggccgcug caccuucgag      780

cccgacgagc cccgggccgc caaggccgcg uacagcuucg aguacuucuc ccugcuccag      840

aagaucaacc acauccgccu gguggagaac ggggagacgc ggccgcugac ccagcagcag      900

cgccagcagc uccugagccu ggcccacaag acccccgacg ugucccucgc ccggauccgc      960

aaggagcugg cccugcccga gaccguccag uucaacggcg ugcggugccg cgcgaacgag     1020

acgcucgagg agagcgagaa gaaggagaag uucgccugcc ugcccgccua ccacaagaug     1080

cggaaggccc uggacggggu ggucaagggc cgcaucucca gccucuccau cagccagcgc     1140

gacgccgcgg ccaccgcccu gagccuguac aagaacgagg acacccuccg ggccaagcug     1200

accgaggccg gcuuccaggc gccggagauc gacgcccugg ccgggcucac gggcuucucc     1260

aaguucgggc accugagccu gaaggccugc cgcaagcuga ucccccaccu cgagcagggc     1320

cugaccuacg accaggccug cuccgcggcc ggguacgacu ucaagggcca cggcgccggg     1380

gagcgggccu ucacccugcc cgcggccgcc cccgagaugg agcagaucac cagcccggug     1440

gugcgccggg ccgucgccca gacgaucaag guggugaacg gcaucauccg cgagauggac     1500

gcgucccccg ccuggguccg gaucgagcuc gcccgcgagc ugagcaagac cuucggggag     1560

cggcaggaga uggaccgcuc caugcgggag aacgccgccc agaacgagcg ccugaugcag     1620

gagcuccggg acaccuucca ccuccugagc cccaccggcc aggaccuggu gaaguaccgc     1680

cuguggaagg agcaggacgg cgucugcgcg uacucccugc ggcgccucga cguggagcgg     1740

cuguucgagc ccggguacgu ggacgucgac cacaucgugc cguacagccu guccuucgac     1800

gaccgccgga gcaacaaggu gcucguccug uccagcgaga accgccagaa gggcaaccgg     1860

cugccccucc aguaccugca ggggaagcgc cgcgaggacu ucaucgugug gacgaacucc     1920

agcgugcggg acuaccgcaa gcggcagaac cugcuccgcg agaagcuguc cggcgacgag     1980

gccgaggggu uccggcagcg caaccugcag gacacccagc acauggcccg guuccucuac     2040

aacuacauca gcgaccaccu ggccuucgcc caguccgagg cgcugggcaa gaagcgcguc     2100

uucgccguga gcggcgccgu gaccagccac cuccggaagc gcugggggcu guccaagguc     2160

cgggccgacg gcgaccugca ccacgcgcuc gacgccgccg ugaucgccug caccacggac     2220

gggaugaucc gccggaucag cggcuacuac gggcacaucg agggcgagua ccugcaggac     2280

gccgacggcg cgggguccca gcacgcccgc accaaggagc gguuccccgc ccccuggccg     2340

cgcuuccggg acgagcugau cgugcgccuc agcgagcagc ccggcgagca ccuccuggac     2400

aucaaccccg aguucuacug cgaguacggg accgagcaca uccggcccgu cuucgugucc     2460

cgcaugccgc gccggaaggu gaccggcccc gggcacaagg agacgaucaa gggcgccgcc     2520

gcggccgacg agggccugcu gaccguccgc aaggcccuga ccgagcucaa gcuggacaag     2580

gacggggaga ucaaggacua cuacaugccc agcuccgaca cccugcucua cgaggcccug     2640

aaggcccagc ugcggcgcuu cggcggggac agcaagaagg cguucgccga gccguucuac     2700

aagcccaagg ccgacggcac gcccgggccc cucgugcgga aggugaagac caccgagaag     2760

gccacccuga ccgucccggu gcacggcggc gccgcgucca acgacacgau ggugcgcguc     2820

gacguguucc uggugcccgg ggacggcuac uacugggucc ccguguacac cgccgacacc     2880

cucaagcccg agcugccgaa ccgggccgug guccagggga agcccuacag cgaguggaag     2940

gagaugcgcg aggaggacuu caucuucucc cuguacccca acgaccucgu guacguggag     3000

cacaagagcg gccugaaguu cacccugcag aacgccgacu ccacgcucga gaagacccgg     3060

acccagacca gcgcguucgu cuacuucgug ggcgggucca ucagcacggc cuccaucacc     3120

auccgcaccc acgacaacgc cuacggcauc cccagccugg gggugaagac ccugaagacg     3180

uuccacaagu accaggucga cgugcucggc aacguguucc cgguccacaa ggagacccgg     3240

cagaaguucc gcuga                                                      3255


<210>  1561
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1561
augaagaaca cccuguacgg caucgggcuc gacaucggcg uggccagcgu cgggugggcc       60

guggugggcc ugaacgggac cggcgagccc gucggccugc accgccucgg ggugcggauc      120

uucgacaagg cggagcagcc gaagacgggc gagucccugg ccgcgccccg ccggauggcc      180

cgcgggaugc ggcgccggcu gcgccggaag gcccuccgcc gggcggacgu guacgcccug      240

cuggagcgca gcggccucuc cacccgggag gcccuggccc agauguucga ggccgggggc      300

cuggaggaca ucuacgcccu ccgcacccgg gcccuggacg agcccgucgg caaggccgag      360

uucagccgca uccugcucca ccuggcccag cggcgcgggu ucaaguccaa ccggcgcacc      420

gcgagcgacg gcgaggacgg gcggcugcuc gccgccguga acgagaaccg ccgccggaug      480

gcccagggcg gcuggcgcac ggugggggag augcuguacc ggcacgaggc cuucgcgcug      540

cacaagcgca acaaggccga cgacuaccuc uccaccgucg gccgggacau gguggccgag      600

gaggccagcc ugcuguucca gcgccagcgg gagcucgggu gcgccugggc gacccccgag      660

cugcaggccg aguaccuguc cauccuccug cgccagcgga gcuucgacga gggccccggg      720

ggcgacuccc cguacggcgg gaaccaggug gagaagaugg ucggccgcug caccuucgag      780

cccgacgagc cccgggccgc caaggccgcg uacagcuucg aguacuucuc ccugcuccag      840

aagaucaacc acauccgccu ggccgagaac ggggagacgc ggccgcugac ccagccccag      900

cgccagcagc uccugagccu ggcccacaag acccccgaca ugucccucgc ccggauccgc      960

aaggagcugg cgcugcccga gaccgugcag uucaacggcg ugcggugccg cgccaacgag     1020

acgcucgagg agagcgagaa gaaggagaag uucgccugcc ugccggccua ccacaagaug     1080

cggaaggccc ucgggggcgu cgugaagggc cgcaucucca gccuguccau cagccagcgc     1140

gacgcggccg ccaccgcccu gagccuguac aagaacgagg acacccuccg ggccaagcug     1200

accgaggcgg gguuccaggc ccccgagauc gacgcccugg ccggccucac gggguucucc     1260

aaguucggcc accugagccu gaaggccugc cgcaagcuga ucccccaccu cgagcagggg     1320

cugaccuacg accaggcgug cuccgccgcc ggcuacgacu ucaagggcca cggggccggc     1380

gagcgggcgu ucacccugcc cgccgccgcc ccggagaugg agcagaucac cagccccgug     1440

guccgccggg ccguggcgca gacgaucaag guggucaacg ggaucauccg cgagauggac     1500

gccucccccg ccugggugcg gaucgagcuc gcccgcgagc ugagcaagac cuucggcgag     1560

cggcaggaga uggaccgcuc caugcgggag aacgccgcga agaacgagcg ccugaugcag     1620

gagcuccggg acaccuucca ccuccugagc cccaccggcc aggaccuggu gaaguaccgc     1680

cuguggaagg agcaggacgg ggucugcgcc uacucccugc agcggcucga cguggagcag     1740

cuguucgagc cgggcuacgu cgacguggac cacaucgugc ccuacagccu guccuucgac     1800

gaccgccgga gcaacaaggu ccucgugcug uccagcgaga accgccagaa ggggaaccgg     1860

cugccccucc aguaccugca gggcaagcgc cgggaggacu ucaucgugug gacgaacucc     1920

agcguccacg acuaccgcaa gcgccagaac cugcuccggg agaagcuguc cgacgacgag     1980

gccgaggggu uccgccagcg gaaccugcag gacacccagc acauggcccg cuuccucuac     2040

aacuacauca gcgaccaccu ggccuucgcg caguccgagg cccugggcaa gaagcgggug     2100

uucgccguga gcggcgccgu caccagccac cuccgcaagc ggugggggcu guccaaggug     2160

cgcgcggacg gcgaccugca ccacgcccuc gacgccgccg uggucgccug caccacggac     2220

gggaugaucc ggcgcaucag cggcuacuac gggcacaucg agggcgagua cuuccaggac     2280

gcggacggcg ccggguccca gcacgcccgg accaaggagc gcuuccccgc cccguggccc     2340

cgguuccgcg acgagcugau cgugcggcug agcgagcagc ccggcgagcc ccuccuggac     2400

aucaacccgg ccuucuacug cgaguacggg accgagcaca uccgccccgu guucgucucc     2460

cggaugcccc gccggaaggu gaccggcccg agccacaagg agacggugaa gggggcggcc     2520

gccgccgacg agggccugcu caccguccgc aaggcccuga ccgagcugaa gcucgacaag     2580

gacggcgaga ucaaggacua cuacaugccc uccagcgaca cccugcugua cgaggcgcuc     2640

aaggcccagc ugcgccgguu cgggggcgac gggaagaagg ccuucgccga gcccuucuac     2700

aagcccaagg ccgacggcac gccggggccc cuggugcgca aggugaagac caccgagaag     2760

gcgacccuca ccguccccgu gcacggcggc gccgccucca acgacacgau ggugcggauc     2820

gacgucuucu ucaugcccgg ggacggcuac uacugggugc cgcuguacgu ggccgacacc     2880

cugaagcccg agcuccccaa ccgcgcgguc guggcccaca agcccuacag cgaguggcag     2940

gagaugcggg aggaggacuu cguguucucc cuguacccga acgaccuggu cuacguggag     3000

cacaagagcg agcucaaguu caccgugcag aacgccgacu ccacccugga gaagacgcgc     3060

acccaggcca gcgccuucgu cuacuucgug uccgggggca ucagcggcgg gaccaucacc     3120

gugcggacgc acgacaacuc cuacggcauc cccagccugg ggcucaagac ccucaagagc     3180

cugugcaagu accaggucga cgugcugggc aacguguucc ccguccacaa ggagacccgc     3240

cagaaguucc gguga                                                      3255


<210>  1562
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1562
augaagaaca cccuguacgg caucgggcuc gacaucggcg uggccagcgu cgggugggcc       60

guggugggcc ugaacgggac cggcgagccc gucggccugc accgccucgg ggugcggauc      120

uucgacaagg cggagcagcc gaagacgggc gagucccugg ccgccccgcg ccggauggcc      180

cgcgggaugc ggcgccggcu gcgccggaag gcccuccgcc gggcggacgu guacgcccug      240

cuggagcgca gcggccucuc cacccgggag gagcuggccc agauguucga ggccgggggc      300

cuggaggaca ucuacgcgcu ccgcacccgg gcccuggacg agcccgucgg caaggccgag      360

uucagccgca uccugcucca ccuggcccag cggcgcgggu ucaaguccaa ccggcgcacc      420

gccagcgacg gcgaggacgg gcggcugcuc gcggccguga acgagaaccg ccgccggaug      480

gcccagggcg gcuggcgcac ggugggggag augcuguacc ggcacgaggc cuucgcccug      540

cacaagcgca acaaggcgga cgacuaccuc uccaccgucg gccgggacau gguggccgag      600

gaggccagca uccuguucga gcgccagcgg gccauggggu gcuccugggc cgcgcccgag      660

cugcaggccg aguaccucag cauccugcug cgccagcggu ccuucgacga gggccccggg      720

ggcaacagcc ccuacggcgg gaaccaggug gagaagaugg ucggccgcug caccuucgag      780

cccgacgagc cccgggccgc caaggccgcg uacuccuucg aguacuucag ccuccugcag      840

aagcugaacc acauccgccu cgccgagaac ggggagaccc ggccgcugac gcagccccag      900

cgccagcagc ugcucucccu ggcccacaag acccccgaca ucagccuggc ccggauccgc      960

aaggagcucg cgcugcccga gaccgugcag uucaacggcg ugcggugccg cgccaacgag     1020

acccuggagg aguccgagaa gaaggagaag uucgccugcc ucccggccua ccacaagaug     1080

cggaaggccc uggacggggu cgugaagggc cgcaucagcu cccugagcau cagccagcgc     1140

gacgcggccg ccacggcccu cucccuguac aagaacgagg acacccugcg ggccaagcuc     1200

accgaggcgg gcuuccaggc ccccgagauc gacgcccugg ccgggcugac cggcuucagc     1260

aaguucgggc accucucccu gaaggccugc cgcaagcuga ucccccaccu ggagcagggc     1320

cucacguacg accaggcgug caccgccgcc ggguacgacu ucaagggcca cggcgccggg     1380

gagcgggcgu ucacccugcc cgccgccgcc ccggagaugg agcagaucac cagccccgug     1440

guccgccggg ccguggcgca gacgaucaag guggucaacg gcaucauccg cgagauggac     1500

gccucccccg ccugggugcg gaucgagcug gcccgcgagc ucagcaagac cuucggggag     1560

cggcaggaga uggaccgcuc caugcgggag aacgccgcgc agaacgagcg ccugaugcag     1620

gagcugcggg acaccuucca ccuccugagc cccaccggcc aggaccuggu gaaguaccgc     1680

cucuggaagg agcaggacgg cgucugcgcc uacucccugc ggcgccugga cguggagcgg     1740

cuguucgagc cgggguacgu cgacguggac cacaucgugc ccuacagccu guccuucgac     1800

gaccgccgga gcaacaaggu ccuggugcuc uccagcgaga accgccagaa gggcaaccgg     1860

cugccccugc aguaccucca ggggaagcgc cgcgaggacu ucaucgugug gacgaacucc     1920

agcguccacg acuaccggaa gcgccagaac cugcugcggg agaagcucuc cggcgacgag     1980

gccgaggggu uccgccagcg gaaccugcag gacacccagc acauggcccg cuuccuguac     2040

aacuacauca gcgaccaccu cgccuucgcg caguccgagg cccugggcaa gaagcgggug     2100

uucgccguga gcggcgccgu caccagccac cuccgcaagc ggugggggcu cuccaaggug     2160

cgcgcggacg gcgaccugca ccacgcccug gacgccgccg uggucgccug caccacggac     2220

gggaugaucc ggcgcaucag cggcuacuac gggcacaucg agggcgagua ccuccaggac     2280

gcggacggcg ccggguccca gcacgcccgg accaaggagc gcuuccccgc cccguggccc     2340

cgguuccgcg acgagcugau cgugcggcug agcgagcagc ccgccgagcc ccuccuggac     2400

aucaacccgg aguucuacug cgaguacggc accgagcaca uccgccccgu guucgucucc     2460

cggaugcccc gccgcaaggu gaccgggccg ggccacaagg agacgaucaa gggggcggcc     2520

gccgccgacg agggccugcu caccgugcgg aaggcccuga ccgagcugaa gcucgacaag     2580

gacggcgaga ucaaggacua cuacaugccc agcuccgaca cccugcugua cgaggcgcuc     2640

aaggcccagc ugcgccgguu cgggggcgac gggaagaagg ccuucgccga gcccuucuac     2700

aagcccaagg ccgacggcac gccggggccc cugguccgca aggugaagac caccgagaag     2760

gcgacccuca ccgugcccgu ccacggcggc gccgccagca acgacacgau ggugcgggug     2820

gacgucuucc uggugcccgg ggacggcuac uacugggugc cggucuacac cgccgacacc     2880

cugaagcccg agcuccccaa ccgcgcggug gugcagggga agcccuacuc cgaguggaag     2940

gagaugcggg aggaggacuu caucuucagc cuguacccga acgaccuggu cuacguggag     3000

cacaaguccg gccucaaguu cacccugcag aacgccgaca gcacgcugga gaagacccgc     3060

acccagaccu ccgccuucgc cuacuucgug agcggcucca ucagcacggc cuccaucacc     3120

auccggaccc acgacaacgc guacgggauc cccagccucg gcgucaagac ccugaagacg     3180

uuccacaagu accaggugga cgugcugggg aacgucuucc ccgugcacaa ggagacccgc     3240

cagaaguucc gguga                                                      3255


<210>  1563
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1563
augcgccugg gccucgacau cgggaccaac agcaucggcu gguggcugua cgggaccgag       60

ggcgggcgga ucacggaggu ggucgacggc ggcgugcgca ucuucuccga cgggcgggac      120

ccccagagca aggccucccu ggccguggac cgccgggcgg cccgcagcca gcggcgccgg      180

cgcgaccggu accuccgccg gaaggccgcc cugaugaagc gcauggccca ggucggccug      240

augccggcgg accccgccga ggccaaggug cucgaggugc ugaaccccua cgagcugcgg      300

gccuccgggc ucgacggccc ccugccgcug acccacuucg ggcgcgcccu cuuccaccug      360

aaccagcggc gcggcuucaa gagcaaccgg aagaccgacc gcggcgacaa cgaguccggg      420

aagaucaagg acgccaccgc ccggcuggac caggccauga uggccaaggg cgcgcgcacg      480

uacggggagu uccuccacau gcgccgggcc gccgcccugg accccaagag cguccccucc      540

gugcgcaccc ggcugaccgu ggcccgccgg gacgacgcgg agaaggagga ggccggcuac      600

gacuucuacc ccgaccgcaa gcaccucagc gaggaguucg acaagcugug ggccgcccag      660

gcccggcacg cgccggacgu ccugaccgag gaccuccgcg acgagaucgc cacgaucauc      720

uuccaccagc ggccccugaa gacccccgag gugggccugu gccucuucac cgggcagcac      780

ggcgugcccg agcgcgacaa gcggaucccg uccgcccacc cccugaacca gcgccggauc      840

cugauggaga ccgucaacaa ccucaaggug gccgcccagg gggagcccag ccgcccccug      900

acgcgggagg agcgcgacca gaucgugcac gcgcuggaca acaagaagca caccaagucc      960

cucagcggca ugaccaugaa gcugaaggcc cuggggaagg ucaucaagcu ccggccggag     1020

cagggcuuca cccuggagac ggccaaccgc gacgccaucg cgugcgaccc cgugcgggcc     1080

ucccugagcc accccgaccg cuucggcgcc cgcuggucca cccucgagag cgccgggcag     1140

ugggacgugg uccagcggau ccgcgccgug caguccgacg cggagcacgc cgaccuggug     1200

ggcuggcuga uggacgucca cgggcucgac cgggaccacg ccgaggagac cgccaacgcg     1260

ccccugccgg agggcuacgg gcgccugggc cucaccgcga cgacccggau ccuggccgcc     1320

cuggaggccg acgugcucaa cuacagcgcc gcgguggccg ccugcggcug gcaccacagc     1380

gacgggcgca ccggcgaggu ccuggagucc cugcccuacu acggggagau ccucgagcgg     1440

cacgugaucc ccggcaccgg ggaccccaac gacgacgacg ucaagcgcua cggccggauc     1500

acgaacccga ccgugcacau cggccugaac cagcugcgcc ggcuggugaa ccgcaucauc     1560

accgucuacg ggcggcccga cgagaucgug guggagcucg cccgcgaccu gaagcugagc     1620

gaggaccaga agaaggacgu ccagcgggac aucaagaaga acaccgaggc ggccauccgc     1680

cggggcaaga agcucgagga gaugggggug cccaacacgg gcgccaaccg cauggugcug     1740

cggcuguggg aggaccucgg cgacgacguc augacccgca acugccccua caccgggaag     1800

cggaucuccg ccggcaugcu guucgacggg agcugcgacg uggaccacau ccugccguac     1860

ucccgcaccc ucgacgacag cuucgccaac cgcacgcugu gccugaagga ggcgaaccgg     1920

cagaagaccg acaagacccc cugggaggcc uggggcgaga ccgagcagug ggaggccauc     1980

gccgccaacc ucaagaaccu gcccgcgaac aaggccuggc gcuucgcccc ggacgccaug     2040

gagcgguucg agggggagaa ggacuucucc gcccgcgcgc uggccgacac gcaguaccuc     2100

agccggaucg cccgcgccua ccuggacgcg cuguacgacg gcgacgacgg caagucccac     2160

gugugggucg ugcccgggcg gcucaccgag augcugcgcc ggcacugggg ccugaacggg     2220

guggccgccc ucaccgacag cgacaagcag accgucaagg ccaagaaccg caccgaccac     2280

cggcaccacg ccaucgacgc ggccguggug gccgccacgg accgcggccu gaucaagcgg     2340

aucgccgaca uggcgaagcg cgacgaguuc aacggggccg aggaggucgc ccgguccauc     2400

ccgccgcccu gggagggcuu ccgcgacgac gugagcgccc aggugacccg gaucaucguc     2460

ucccaccgcg ccgaccacgg ccggaucgac cccgcggccc gcaagagcgg gcgcgacucc     2520

accagcggcc agcugcacaa cgacaccgcc uacgggaucg gccgggacgg gacgguggug     2580

ucccgcaagc cccucgccag ccuguccccc aacgacaucg ucgugaccaa gcggggcgcc     2640

aacauccgcg acguggaccu gcagaagcac cucgcgcggg ucacccacgg ccuggacggg     2700

aaggccuucg aggccgcccu gcucgcguuc gccgccagcc ccauccugcc cgacaacacc     2760

gacaaccccu acuucggccu gcgccgggug cgccucgagg agacgcugca ggagagcgcc     2820

cggguggagg ucgccgacca ggaggggcgc cccuacaagg cguacaaggg cgacuccaac     2880

cacugcuacg agaucuggug ccugccggac gggaagauca agccccaggu ggugaccacc     2940

uacgaggccc accagagcgg cgccgagaag aagccccacc ccgccgccaa gcggcucaug     3000

cgcaucuuca agcgggacau ggucaugcug gagcgcgacg gcgagaccau caucggguac     3060

gugcagaaga ugaagcagaa cggcuccauc uucguggcgc cgcacacgga ggccaacgcc     3120

gacgcccggg accgcgaccc caaggacgac uucaagcuga uccagcucgg ggccggcagc     3180

cuggucaagg cgaaggcccg gcgcgugauc guggacgaga ugggccggcu gcgcgacccc     3240

gggcccccgc ucuga                                                      3255


<210>  1564
<211>  3258
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1564
auggacaagg gcaccaacag caucgggugg gccgcccugc gccucgacga gcagggcgag       60

cccgugggga ucaucgaggc gggcguccgg aucuacgccg acgggcgcga gccgaagucc      120

ggcaccagcc uggccgaggg ccggcgcauc gcccggggga ugucccgccg gcgcgaccgg      180

uacaagcgcc ggcgccggac ggugcugaac acccucaccg aguucggccu gaugcccgcc      240

accaaggcgg cccgcaaggc ccugguggcc gagacgaacg accggcgcuu cggggagguc      300

gcgagcaacg uguacgcccu ccgggcccgc gcccuggacg agaagcugcc ccucuaccag      360

cugggccggg cccuguucca ccucaaccag cgccgggggu ucaaguccaa ccgcaagacc      420

gacaagcgcg acaacgacca gggcaagauc gcgagcgcca ucucccggcu gggccgcaag      480

cuggacgagg ccggggcccg gacccucggc gaguuccugc acaagcagca cgccgcgggg      540

aagugggugc gcguccggcc cagccgcgug ccgggcgagg acgacaagga ggaggacggc      600

uacggguucu accccgagcg guccuuccug gagcgcgagu ucgccgccau cugggaggcc      660

caggccgcgu ucuaccccga gcuccugacc gaggagcggc gcgaccaccu ccacugggug      720

auguucuucc agcggccccu gaagcgcccg acggucggca agugcagcuu ccaccccgac      780

gaggaccggc ugcccaaggc ccacccccug uuccaggagu uccgccucau caaggagcug      840

aacgagcugg agguggugca ccgcggggac cggcgggaga agcucacccc ggaccagcgc      900

gaccggcugu ucaaccagcu gcgcacccag cgcuccagcg ccuucaccgc ccuccggaag      960

acgaucaagg cccccaccgg cacccgcuuc aacaaggagu ccgacgcgcg ggacaagcug     1020

aagggggacg aggucgccgc cgagaugagc cacaaggacc gcuucggcca ggccuggauc     1080

ggcaugcccc uggcggagcg gugggccgug guggccaugc uccuggacac cgaggacccc     1140

cuggagguca aggccuggcu cgaggagcgc uacggguucg ccgcggaccg gaucgacgcc     1200

aucgccgccg ccaagcugcc gacgggcuac gggcgccugg gcgagaccgc gcuccgggac     1260

augcuggacg agcugcgcaa cgggaccgug aaggacgacg ccggccggga cgugguccuc     1320

accgagcgcc aggccaugga caacaucugg ggcgacaacc ccgcccugca gcgggccgug     1380

acggacgcgc gcgagcugcc gcccuaccag gaggugcucg ggcggcgcau cccgcccggc     1440

accgggaagc ccgccgaccc cuacgacguc cagaagggcc ggcugaccaa ccccaccgug     1500

cacaucgggc ugaaccagcu caagcgcauc gugaacgucc ugaugcggcg ccacggccgg     1560

cccgagcaga ucgcccugga gcuggcccgc gaccucaagc ugaacgagga gcagaaggcg     1620

gagaagaacc gcgagaucgc ccggaacacg aaggccgccg aggcccgcgg caagaagcug     1680

gaggagcucg ggcacgcgaa caacggcuac aaccgggugc ugcugaagcu cugggaggac     1740

cugaaccuga agcagcccga gaaccgcgag ugcaucuacu gcgggaaggc caucggcauc     1800

ggcacccucc uguccaacca ggucgacauc gaccacaucc ugcccuggag caagacccuc     1860

gacgacuccc aggccaaccg gaucgugugc cacguggagu gcaaccgcca gaaggggaac     1920

cacgccccgg ccgagguccc ccaguggcac gaccgguacg acgacauccu ggcgcgcgcc     1980

gagcggcugc cccgcaacaa ggccuggcgg uucggccgcg aggccaugaa gaaguucaag     2040

gacgacgaga gcuuccucgc ccggcagcug accgacacgc aguaccuguc ccgccucgcg     2100

cacgaguacc ugggggcccu guacccggac gaggagcccg acgagugggg cgggcccgcc     2160

cggcgcaacc acgugcgggu gauccccggc cgcaugaccg agaugcuccg gcgccagugg     2220

ggccugaacg ccaucaucag cgaccacaac cagaagaacc gcaacgacca ccggcaccac     2280

gcgaucgacg ccaucgucgu gggggugacc acccgccggc ugcuccagcg caucgccacg     2340

gucgccggcg ggcgggaggc ccuggcgcug gaggagacca ucggcaagau cgagccgccc     2400

uggcccgggu ucuacgacga cgugaaggcc gccaucgacc gcaucguggu cagccacaag     2460

cccgaccacg gcucccuccc gaagcccggc aagcccgggc ggaccgccgg ccagcugcac     2520

aacgacaccg ccuacggguu cacgggcgag gcgcgcaacg gggugcccau cauggugcac     2580

cgggucccgu ucgccagccu gaccgagaag gacauccccu ccauccgcga ccggcagcuc     2640

gccgacgccc uguacgccgc gaucagcggc aucuccggca aggccgagcu ggccgccgcc     2700

cucgcgcgcu ucagcgaguc cgggcccuac aagggcaucc ggcacgugcg cguggucgag     2760

cccgugagcg ugaucgccau caaggaccag gccgggaagg ccuacaaggg cuacaagggg     2820

gacgcgaacu accgguacga cgucugggag cugccgaacg gcaaguggac ccacgaggug     2880

guguccaugu ucaacgccca ccgccccgac uggcagagcg agauccgguc caagaacccc     2940

gccgcccgca agguccugag ccuccaccag aacgacaugg uggccuacga gcacccccag     3000

gacggcuaca ccaucgggcg gguggucaag uucuccgcgg gcgggcagau ggcccuggcc     3060

ggcaaccgcg agggcggggc ccucaagagc cgggacgccg acccggagga ccgcuuccgc     3120

uacuucuaca aguccggcgg ggcgaugcgg gacgugcagc ugcgccaggu gcgggucgac     3180

gagaccggcc gcguguucga ccccgggccc caggaccggg agagccgcaa cgcccggaag     3240

cagaacgacg ccggcuga                                                   3258


<210>  1565
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1565
augcgcuaca agaucggccu ggacaucggg aucaccagcg ugggcugggc cgucaugaac       60

cucgacaucc cccggaucga ggaccugggg gugcgcaucu ucgaccgggc cgagaacccg      120

cagaccggcg agucccuggc gcucccccgc cggcuggccc gcagcgcccg gcgccggcug      180

cgccggcgca agcaccggcu cgagcgcauc cggcgccugg ugauccggga ggggauccug      240

acgaaggagg agcucgacaa gcuguucgag gagaagcacg agaucgacgu cuggcagcug      300

cgcguggagg cccucgaccg gaagcugaac aacgacgagc uggcccgcgu gcuccugcac      360

cuggcgaagc ggcgcggcuu caaguccaac cgcaagagcg agcgguccaa caaggagaac      420

agcaccaugc ucaagcacau cgaggagaac cgcgccaucc uguccagcua ccggaccguc      480

ggcgagauga ucgugaagga ccccaaguuc gcccugcaca agcgcaacaa gggggagaac      540

uacaccaaca cgaucgcccg ggacgaccuc gagcgcgaga uccggcugau cuucuccaag      600

cagcgcgagu ucggcaacau gagcugcacc gaggaguucg agaacgagua caucaccauc      660

ugggcguccc agcggcccgu ggccagcaag gacgacaucg agaagaaggu cggguucugc      720

accuucgagc cgaaggagaa gcgcgccccc aaggccacgu acaccuucca guccuucauc      780

gccugggagc acaucaacaa gcugcggcuc aucagccccu ccggcgcgcg cgggcugacc      840

gacgaggagc ggcgccugcu cuacgagcag gccuuccaga agaacaagau caccuaccac      900

gacauccgga cgcugcugca ccuccccgac gacaccuacu ucaagggcau cguguacgac      960

cgcggcgaga gccggaagca gaacgagaac auccgcuucc uggagcugga cgccuaccac     1020

cagauccgga aggccgugga caaggucuac gggaagggca aguccagcuc cuuccucccg     1080

aucgacuucg acaccuucgg guacgcccug acccuguuca aggacgacgc ggacauccac     1140

agcuaccucc gcaacgagua cgagcagaac ggcaagcgca ugcccaaccu ggccaacaag     1200

guguacgaca acgagcugau cgaggagcuc cugaaccuga gcuucacgaa guucggccac     1260

cucucccuga aggcccugcg gagcauccuc cccuacaugg agcaggggga gguguacucc     1320

agcgccugcg agcgcgccgg cuacaccuuc accgggccca agaagaagca gaagaccaug     1380

cugcugccga acaucccgcc caucgcgaac cccgucguga ugcgggcccu cacgcaggcc     1440

cgcaaggugg ucaacgccau caucaagaag uacggcuccc ccgugagcau ccacaucgag     1500

cuggcccggg accuguccca gaccuucgac gagcgccgga agaccaagaa ggagcaggac     1560

gagaaccgca agaagaacga gaccgcgauc cggcagcuga uggaguacgg gcucacgcug     1620

aaccccaccg gccacgacau cgugaaguuc aagcugugga gcgagcagaa cggccgcugc     1680

gccuacuccc uccagcccau cgagaucgag cggcugcugg agcccgggua cgucgaggug     1740

gaccacguga ucccguacag ccgcucccuc gacgacagcu acaccaacaa gguccuggug     1800

cugacccggg agaaccgcga gaagggcaac cggauccccg ccgaguaccu cggggucggc     1860

acggagcgcu ggcagcaguu cgagaccuuc gugcugacca acaagcaguu cuccaagaag     1920

aagcgggacc gccugcuccg gcugcacuac gacgagaacg aggagaccga guucaagaac     1980

cgcaaccuga acgacacgcg guacaucagc cgcuucuucg ccaacuucau ccgcgagcac     2040

cucaaguucg cggaguccga cgacaagcag aagguguaca ccgucaacgg gcgggugacc     2100

gcccaccucc gcagccggug ggaguucaac aagaaccgcg aggaguccga ccugcaccac     2160

gccguggacg ccgucaucgu ggccugcacc acgcccagcg acaucgcgaa ggugaccgcc     2220

uucuaccagc ggcgcgagca gaacaaggag cuggccaaga agaaggagcc ccacuucccg     2280

cagcccuggc cccacuucgc cgacgagcug cgggcccgcc uguccaagca ccccaaggag     2340

agcaucaagg cgcucaaccu gggcaacuac gacgaccaga agcuggagag ccuccagccg     2400

gucuucgugu cccggaugcc caagcgcagc gugaccggcg ccgcccacca ggagacccug     2460

cggcgcuacg ucgggaucga cgagcggucc ggcaagaucc agaccguggu gaagacgaag     2520

cugagcgaga ucaagcucga cgccuccggg cacuucccca uguacggcaa ggagagcgac     2580

ccgcgcaccu acgaggccau ccggcagcgc cugcuggagc acaacaacga ccccaagaag     2640

gcguuccagg agccccucua caagcccaag aagaacgggg agccgggccc cgucauccgg     2700

accgugaaga ucaucgacac caagaaccag gugauccccc ugaacgacgg caagacgguc     2760

gccuacaacu ccaacaucgu gcgcguggac gucuucgaga aggacgggaa guacuacugc     2820

gugcccgugu acaccaugga caucaugaag ggcauccugc cgaacaaggc caucgagccc     2880

aacaagcccu acagcgagug gaaggagaug accgaggacu acaccuuccg guucucccuc     2940

uaccccaacg accugauccg caucgagcug ccgcgcgaga agacggucaa gaccgccgcg     3000

ggggaggaga ucaacgugaa ggacguguuc gucuacuaca agaccaucga cagcgccaac     3060

ggcggccucg agcugaucuc ccacgaccac cgguucagcc ugcgcggggu gggcucccgg     3120

acccucaagc gcuucgagaa guaccaggug gacguccugg ggaacaucua caaggugcgg     3180

ggcgagaagc gcguggggcu ggccagcucc gcccacagca agcccggcaa gacgauccgg     3240

ccccuccagu ccacccgcga cuga                                            3264


<210>  1566
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1566
augcugcgca ugaaguacgc caucggccuc gacaucggga ucgccagcgu gggcuacgcg       60

guccuggccc uggaccacga ggagaacccc ugggggauca uccggcucgg cucccgcauc      120

uucgacgugg ccgagaaccc gaaggacggg gccagccugg cccugccccg gcgcgaggcg      180

cgguccgugc gccggcgccu ccggcgccac caccaccggc uggagcgcau caagaaccug      240

cucaucaaca ccgaguucau caccaaggac gagcugcugc accucuacga cggcaaccug      300

agcgacgucu acgagcugcg ggugaaggcc cucgacuacu ccgugacgaa cgccgagcug      360

acccgcaucc ugcuccaccu ggcccagcgg cgcggcuuca agagcaaccg gaaguccgac      420

gcgaacgaca aggaggccgg gcagcugcuc gaggccguca gcgccaacgc caagcgcaug      480

caggagaacc acuaccggac cgugggcgag auguucuaca aggacgaccu guucuccaag      540

uacaagcgca acaagggcgg gaccuaccug acgaccgugc accgggacau gaucgcggcc      600

gaggcccgca ccauccucga gaagcaguuc gcccugggga acaacaucug cacccaggac      660

uucaucgaca aguaccugag cauccuccug ucccagcgcc aguucgacga gggccccggc      720

gagcccagcc cguacgccgg gaaccagauc gcgaacauga ucggcaagug cacguucgag      780

cccaacgagu accgggccgc caaggccucc uacagcuucg agcgcuucaa ccugcuccag      840

aaggucaacc accuccggcu gcugcuggag gggaagucca ucgcccugga caacgagcag      900

cgcaagaaga ucaucgcgcu cgcccacgac aaggccgacc ugcgguacag ccacauccgc      960

aaggcccugg gccucgacga gaaggugcug uucaacacca ucaccuacaa cgacgacgug     1020

uccgucaucg agaagaagac caaguucaac uuccugcagg ccuaccacca gaucaagaag     1080

gugcucgcgg aggacaugca gaagcugacg accgagcagc uggacaacau cggccagauc     1140

cucagcaccu acaaguccga caacaagcgg accgaggagc ugagcgcccu ggggcucgag     1200

aagaagauca ucgacgcccu gcugggcauc aacgggcuga gcaaguucgc ccaccucucc     1260

cugaaggcgc ugcgcaagau caaccccuac cucgaggagg gcaagaucua caacgaggcc     1320

ugcacggccg ccggguacga cuucaaggcc cacgcgaaca cccagaagac cgagcugcug     1380

cccgccuaca aggaggagau ggacgacauc accagcccgg uggcccggcg cgccaucgcc     1440

caguccauca aggucaucaa cgcgaucauc cgggagcaga agggcagccc ccucuacauc     1500

aacaucgagc uggcccgcga gauggccaag ggcuucgacg agcggacgca gaucgacaag     1560

gccaacaagg agaaccaggc caagaacgag cgcaucaugg agcggauccg caccgaguuc     1620

cacaaggcga accccaccgg gauggaccug aucaagcuca agcuguggga ggagcaggac     1680

ggccgguccc ccuacagcca gaaggccauc uccaucaacc gccuguucga gccgggguac     1740

guggacaucg accacaucgu gcccuacagc aucuccuucg acgacagcuu caagaacaag     1800

guccucgugu ucuccgacga gaaccgggac aagggcaacc gccugcccau cgccuaccug     1860

cagagcaagu ucggggccaa ggcggccgag aacuucauga ucugggugca guccaacauc     1920

aaggacuaca agaagcgcca gaagcuccug aagcgggaga ucaccgagga ggacaucaac     1980

aaguucaagg agcgcaaccu gcaggacacg aagaccauca gccgguuccu cuacaacuac     2040

aucaacgacu accugcuguu cgcccccucc gacaccggca agaagaagcg cgucaccgcc     2100

gugaacggca cgaucaccgc cuaccuccgg aagcgcuggg ggaucaacaa gauccgggcg     2160

aacggcgaca agcaccacgc cguggacgcc gucgugaucg ccugcaccac cgacaagaug     2220

aucaaggacc ugagcuccuu cagccgcuac cacgagcugg aguacacgca caccgacacc     2280

gaguccuucc ucgucaacag ccugaccggg gagauccuga agcgguuccc guaccccugg     2340

gaggacuucc gccccgagcu cauggcccgg cugagcgaca accccgcgga cgcccugcgc     2400

aagcucaacc ugaucuucua ccacggcauc gaccugucca cgaucaagcc gaucuucgug     2460

agccggaugc cccgccacaa ggugaccggg gccgcccaca aggccaccau caaguccgcg     2520

cggugccucg acaacggcau cgucaucugc aagaccagcc ugcagaaccu gaagcucgac     2580

aaggagggcg agaucgccaa cuacuacgcc cccgaguccg acaccauccu guacaacgcc     2640

cugaaggagc gccuccggag cuacgacaac aagcccgcga aggccuucgc cgagccguuc     2700

uacaagccca aggccgacgg gacgcccggc ccccugguga agaaggugaa ggucuacgag     2760

aaguccaccc ugaacguggc cgugcagcag aacaccgcgg ucgccgacaa cgacagcaug     2820

gugcgcgugg acgucuucca cgugaagggg aacggcuacu accucgugcc gaucuacauc     2880

gccgacaccc ugaagaagga gcugcccaac aaggccaucg ucgccuacaa gcccuacgcg     2940

gacuggccgg ugauggacga cuccaacuuc aucuucagcc ucuaccccaa cgaccugauc     3000

aaguucgagc acaagaacuc cgugaaguuc gccaagguca acaaggagag cacgcugaac     3060

gacuucuacu ccaccaagag cgagaucgug uacuucaagg gcugcaacau caacaccggg     3120

gccaucacca ucaucaacca cgagaacacg uacguggucu ccagccucgg cgugaagacc     3180

cugcccuccc uggagaagua ccaggccgac gugcucggga acuacaccaa ggucaagaag     3240

gaggugcgga agcccuuccg cuga                                            3264


<210>  1567
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1567
augcgcuaca agaucggccu ggacaucggg aucaccagcg ugggcugggc cgucaucaac       60

cucgacaucc cccggaucga ggaccugggg gugcgcaucu ucgaccgggc cgagaacccg      120

cagaccggcg agucccuggc gcucccccgc cggcuggccc gcagcgcccg gcgccggcug      180

cgccggcgca agcaccggcu cgagcgcauc cggcgccuga ucauccggga ggggauccug      240

acgaaggagg agcucgacaa gcuguucgag gagaagcacg agaucgacgu guggcagcug      300

cgcgucgagg cccucgaccg gaagcugaac aacgacgagc uggcccgcgu gcuccugcac      360

cuggcgaagc ggcgcggcuu caaguccaac cgcaagagcg agcgguccaa caaggagaac      420

agcaccaugc ucaagcacau cgaggagaac cgcgccaucc uguccagcua ccggaccgug      480

ggcgagauga ucgucaagga ccccaaguuc gcccugcaca agcgcaacaa gggggagaac      540

uacaccaaca cgaucgcccg ggacgaccuc gagcgcgaga uccggcugau cuucuccaag      600

cagcgcgagu ucggcaacau gagcugcacc gaggaguucg agaacgagua caucgcgauc      660

ugggccuccc agcggcccgu ggccagcaag gacgacaucg agaagaaggu gggguucugc      720

accuucgagc cgaaggagaa gcgcgccccc aaggccaccu acacguucca guccuucauc      780

gcgugggagc acaucaacaa gcugcggcuc aucagccccu ccggcacccg cgggcugacc      840

gacgaggagc ggcgccugcu cuacgagcag gccuuccaga agaacaagau caccuaccac      900

gacauccgga cgcugcugca ccuccccgac gacaccuacu ucaagggcau cgucuacgac      960

cgcggcgaga gccggaagca gaacgagaac auccgcuucc uggagcugga cgccuaccac     1020

cagauccgga aggccgugga caagguguac gggaagggca aguccagcuc cuuccucccg     1080

aucgacuucg acaccuucgg guacgcccug acccuguuca aggacgacgc ggacauccgc     1140

agcuaccucc gcaacgagua cgagcagaac ggcaagcgga ugcccaaccu ggccaacaag     1200

gucuacgaca acgagcugau cgaggagcuc cugaaccuga gcuucacgaa guucggccac     1260

cucucccuga aggcccugcg cagcauccuc cccuacaugg agcaggggga gguguacucc     1320

agcgccugcg agcgggccgg cuacaccuuc accgggccca agaagaagca gaagaccaug     1380

cugcugccga acaucccgcc caucgcgaac cccgugguca ugcgcgcccu cacgcaggcc     1440

cggaaggugg ugaacgccau caucaagaag uacggcuccc ccgucagcau ccacaucgag     1500

cuggcccgcg accuguccca gaccuucgac gagcggcgca agaccaagaa ggagcaggac     1560

gagaaccgga agaagaacga gaccgcgauc cgccagcuga uggaguacgg gcucacgcug     1620

aaccccaccg gccacgacau cgugaaguuc aagcugugga gcgagcagaa cggccggugc     1680

gccuacuccc uccagcccau cgagaucgag cgccugcugg agcccgggua caccgaggug     1740

gaccacguca ucccguacag ccggucccuc gacgacagcu acaccaacaa ggugcuggug     1800

cugacgaagg agaaccgcga gaagggcaac cggauccccg ccgaguaccu cggggucggc     1860

accgagcgcu ggcagcaguu cgagaccuuc gugcugacca acaagcaguu cuccaagaag     1920

aagcgggacc gccugcuccg gcugcacuac gacgagaacg aggagacgga guucaagaac     1980

cgcaaccuga acgacacccg guacaucagc cgcuucuucg ccaacuucau ccgcgagcac     2040

cucaaguucg cggaguccga cgacaagcag aaggucuaca ccgugaacgg gcgggugacc     2100

gcccaccucc gcagccggug ggaguucaac aagaaccgcg aggaguccga ccugcaccac     2160

gccgucgacg ccgugaucgu ggccugcacg acccccagcg acaucgcgaa ggucaccgcc     2220

uucuaccagc ggcgcgagca gaacaaggag cuggccaaga agaccgagcc ccacuucccg     2280

cagcccuggc cccacuucgc cgacgagcug cgggcccgcc uguccaagca ccccaaggag     2340

agcaucaagg cgcucaaccu gggcaacuac gacgaccaga agcuggagag ccuccagccg     2400

guguucgugu cccggaugcc caagcgcagc gucaccggcg ccgcccacca ggagacgcug     2460

cggcgcuacg uggggaucga cgagcggucc ggcaagaucc agaccguggu caagaccaag     2520

cugagcgaga ucaagcucga cgccuccggg cacuucccca uguacggcaa ggagagcgac     2580

ccgcgcaccu acgaggccau ccggcagcgc cugcuggagc acaacaacga ccccaagaag     2640

gcguuccagg agccccucua caagcccaag aagaacgggg agccgggccc cgugauccgg     2700

acggugaaga ucaucgacac caagaaccag gucauccccc ugaacgacgg caagaccgug     2760

gccuacaacu ccaacaucgu gcgcgucgac guguucgaga aggacgggaa guacuacugc     2820

gugcccgucu acaccaugga caucaugaag ggcauccugc cgaacaaggc caucgagccc     2880

aacaagcccu acagcgagug gaaggagaug acggaggacu acaccuuccg guucucccuc     2940

uaccccaacg accugauccg caucgagcug ccgcgcgaga agaucaucaa gaccgccggg     3000

ggcgaggaga ucaagaucaa ggaccucuuc gcguacuaca agaccaucca cagcggcacg     3060

gccgggcugg agcugguguc ccacgacugc agcuucuccc ucagcggcgu ggggucccgg     3120

acccugaagc gcuucgagaa guaccagguc gacgugcugg gcaacaucua caaggugcgc     3180

ggggagaagc gggucggccu cgccagcucc gcccacagca agaccggcga gaccauccgg     3240

ccccugcaga gcacgcgcga cuga                                            3264


<210>  1568
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1568
augcgcuaca agaucggccu ggacaucggg aucaccagcg ugggcugggc cgucaucaac       60

cucgaccugc cccggaucga ggaccugggg gugcgcaucu ucgaccgggc cgagaacccg      120

cagaccggcg agucccucgc gcugccccgc cggcuggccc gcagcgcccg gcgccggcuc      180

cgccggcgcc ggcaccgccu ggagcggauc cgccggcugc ucacgcgcga gaacgugcug      240

acccaggagg agcuggacaa gcuguucgag aagaagcacg agaucgacgu cuggcagcug      300

cggguggagg cccuggaccg caagcucaac aacgacgagc uggcccgggu gcugcuccac      360

cuggcgaagc gccgcggguu caaguccaac cgcaagagcg agcgguccaa caaggagaac      420

agcaccaugc ugaagcacau cgaggagaac cgguccaucc ucaccggcua ccgcacgguc      480

ggcgagauga ucgugcggga ccccaaguuc gugcugcaca agcgcaacaa gggggagaac      540

uacaccaaca ccaucgcccg ggacgaccug gagcgcgaga uccagcucau cuucgcccgg      600

cagcgcgagu ucggcaacau gaucugcacc gaggccuucg agaacgagua caucgcgauc      660

uggagcuccc agcggcccgu cgccagcaag gacgacaucg agaagaaggu gggguucugc      720

acguucgagc cgaaggagaa gcgcgccccc aaggccaccu acaccuucca guccuucauc      780

gccugggagc acaucaacaa gcugcggcug gugagccccu ccggcacccg cgagcucacg      840

gacgaggagc ggcgccugcu guacgagcag gcguuccaga agaacaagcu caccuaccac      900

gacauccgga cccugcugca ccuccccgac gacaccuacu ucaaggggau cgucuacgac      960

uggggcgagc cgcugaagca gaacgagaac auccgcuucc uggagcucga cgccuaccac     1020

cagauccgga agacggugga caagguguac ggccgcggca agagcuccag cuuccugccc     1080

aucgacuucg acaccuucgg guacgcccug acccucuuca aggacgacgc ccacauccgc     1140

uccuaccugc ggaacgagua cgagcagaac gggaagcgca ucgccaaccu ggcgaacaag     1200

gucuacgaca acgagcucau cgaggagcug cugaaccuca gcuucaccaa guucggccac     1260

cugucccuga aggcccuccg gaacauccug cccuacaugg agcagggcga gguguacagc     1320

agcgccugcg agcgcgccgg guacacguuc accggcccca ccaagaagca gaagaccgug     1380

cugcugccga cgaucccgcc caucgccaac cccgucguga ugcgggcgcu cacccaggcc     1440

cgcaaggugg ucaacgccau caucaagaag uacggguccc ccgugagcau ccacaucgag     1500

cuggcccggg accuguccca gaccuucgac gagcgcaaga agaccaagaa ggagcaggac     1560

gagaaccgga agcgcaacga gacggccauc cggcagcuca ucgaguacgg ccugacccgc     1620

aaccccaccg ggcacgacau cgugaaguuc aagcugugga gcgagcagaa cggccggugc     1680

gcguacuccc uccagcccau cgagaucaag cgccugcugg agcccggcua cgucgaggug     1740

gaccacguca ucccguacag ccggucccuc gacgacagcu acaccaacaa ggugcuggug     1800

cugacgaagg agaaccgcga gaaggggaac cggauccccg ccgaguaccu cggcgucggg     1860

accgagcgcu ggcagcaguu cgagaccuuc gugcugacca acaagcaguu cuccaagaag     1920

aagcgggacc gccugcuccg gcugcacuac gacgagaacg aggaggccga guggaagaac     1980

cgcagccuga acgacacgcg cuacaucucc cgguucuucg ccaacuucau ccgcgagcac     2040

cucaaguucg cggacagcga cgacaagcag aagguguaca ccgucaacgg cggggugacc     2100

gcccaccucc ggucccgcug ggacuucaac aagaaccggg aggagagcga ccugcaccac     2160

gccguggacg ccgccaucgu cgcgugcacc acccucuccg acaucgcccg cgugacggcc     2220

uucuaccagc ggcgcgagca ggccaaggag cuggccaaga agaccgagcc ccacuucccc     2280

cagccguggc cccacuucgc ggacgagcug cgggcccgcc ucagcaagaa ccccaaggag     2340

uccaucaagg cccugaaccu gggcaacuac gacaacgaga agcucgagag ccugcagccc     2400

guguucgucu cccggaugcc gaagcgcagc gugaccggcg ccgcccacca ggagacccug     2460

cggcgcuaca ucgggaucga cgagcggagc ggcaagaucc agacgguggu caagaccaag     2520

cucuccgaga ucaagcugga cgcgagcggg cacuucccca uguacggcaa ggaguccgac     2580

ccccgcaccu acgaggccau ccggcugcgc cuccuggagc acaacaacga cccgcggaag     2640

gccuuccagg agccccugua caagcccaag aagaacgggg agcucggccc caucauccgc     2700

accgugaaga ucaucgacac gaagaaccag gugaucccgc ugaacgacgg caagaccguc     2760

gccuacaaca gcaacaucgu gcgcguggac gucuucgaga aggacgggaa guacuacugc     2820

gugcccgugu acaccaugga caucaugaag aagguccugc ccaacaaggc gaucgagccc     2880

aacaagccgu acuccgagug gaaggagaug accgaggacu acacguuccg guucagccuc     2940

uaccccaacg accugauccg caucgagcug ccccgggaga agaccgugaa gaccaccgcc     3000

ggccacgaga ucaacaucaa ggacguguuc gucuacuaca agacgaucga cuccgccacc     3060

gggggccucg agcugaucag ccacgacaac uccuucagcc ugcgcggcau cgggucccgg     3120

acccucaagc gcuucgagaa guaccaggug gacgugcugg gcaacaucua caagguccgc     3180

ggggagaagc gggugggccu ggccagcucc gcccaccccg agacccggga gacgauccgc     3240

ccgcuccaga gcacccggga cuga                                            3264


<210>  1569
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1569
augaagaagc uggugcucgg ccuggacauc ggggucgcca gcgugggcug ggggaucauc       60

gacgccgaga acggcgacgu ggucgacacc ggggugcgcc uguucuccag ccgggacgcg      120

uccgagaacg agagccgccg gaccucccgc cagagccggc gccucucccg gcgcaagaag      180

caccggcgcg agcggaucaa gcagcugcug cucgcccacg acauggacau cugcgacauc      240

gugaacgaga accccuacga gcugcgcguc aagggccuga acgagaagcu cgaggugagc      300

gagaucuacg uggcccugac gcaccuggcc aagcggcgcg gcgucuccua ccucgacgac      360

gugguggagg agggcgggac caucaagagc gagcaccuga acaagaacau ggaggagauc      420

aaguccggga agaugccgug cgagauccag cuggagcggu ucgagaagua cggccagguc      480

cgcgggauga ucgagaucaa ccaggacacc gaggagagcu ccuuccucgu gaacaucuuc      540

cggaccagcg acuacaucaa ggaggccaag acgauccugg cgacccagca gaaguuccac      600

aagcagauca ccaacgaguu caucgacgag uacauccaca ucauggccuc caagcgcgac      660

uacuucaucg gccccggcaa cgagaagucc cggaccgacu acggggugua ccgcacgaac      720

ggcgacaccu gggacaaccu guucgagauc cucaucggga agugcagcgu cuuccccgag      780

gagacccggg ccgcccgcag cuccuacgug gcgcaggagu ucaaccugcu gaacgaccuc      840

aacaaccuga agaucagcaa ccuggagagc ggcaagcucu ccgccagcca gaagcacgac      900

aucguggaga aggucaagaa cuccaagaag uucggcacca aggaccugcu gaaguacauc      960

gccagcgugu gcgagugcaa gaucgaggac aucgggggcu gccgcgugga caagaaccgg     1020

aagcccauca ugcacacguu cgagaacuac cgcaaggucc acaacgcccu caacgaggcc     1080

gggguggacc ugcggcuguc cgagaccgac uacgacgagc ucaugaagau caugacccug     1140

aacagcgaga cccuggagau caagaagcag ugcaaggaga agcucccgca gcugacggag     1200

gacuaccuga acgugugccu cguccugaac aagaagggcg cguucuccgg guggcacgcc     1260

cugagccuca aggccaugaa gcugaugaug cccgagcugu acggcaccuc caagaaccag     1320

augcagauca uccacaccau gcagcucggc aaggccuacc ugaagccccu gaaccagcag     1380

aaguaccuca acagcgaggc cauccuggag gagaucuaca accccaucgc ggcccgcucc     1440

auccggcaga ccaucaaggu gacgaacgcc auccugaaga aguacgggca ccucgacagc     1500

gucaucaucg agaugccgcg cgacuaccag accuccgagg acgagcggaa gaugaucgag     1560

aagaugcaga aggagaacga gaaggagaag aaggccgccc ugaagcgcgc gcgggaggag     1620

uaccccuucc ccgacgaggc cuuccgcaac cacaaggagc uggccaccaa gcugcggcuc     1680

ugguaccagc aggaccaguu cugcguguac agcggccgca agaucuccgu ggucgaccug     1740

gugcggaacc ccaaccaguu cgagaucgac cacaucaucc cgcagagcgu gucccuggac     1800

gacagccuca acaacaaggu ccugugcuuc uccgaggaga accagaagaa ggggcagcag     1860

acccccuucu acuaccacaa gggcaagagc gggaacugga cguacgagca guacaagcug     1920

uucguggaga agcuguucga caagaagaag aucagcaaca agaagcgcga gcugcuccuc     1980

uucgaggagg acaucaacaa gugggacgug cggcgcggcu ucaucgcccg gaaccugguc     2040

gacacccgcu acgccucccg ggugauccug aacagcuacc agcaguacuu cggcaaccac     2100

gagaugggga ccaaggugaa ggucgugcgc ggcaaguuca ccucccagcu ccgcaagaag     2160

uggcggaucu ucaaggaccg caacgagagc cacucccacc acgcgaucga cgcccugauc     2220

guggccgcca cggcgaaccu gcggcucugg aagaagaaca cccugggggu cagcgacgag     2280

ggcaaggugu acgacaagga gaccggggag auccugcccu ucgagaagcu caccgagucc     2340

gaguacaacg agaugaugaa caaggagccc uaccugaacu ucaagacgaa gcugcagaac     2400

aucgagacca aggugaagua cagcuaccgc gucuccaaga aggugaaccg gaagaucagc     2460

gacgccacca ucuacuccac ccgcgagcgg gacggcaucg acuacaucgu gggcaagauc     2520

aagaacaucu acgacgucaa gacguacaag gaccaguucc ucaagcgcua caagaagaac     2580

aaggagcagu uccugaugua ccagcacgac ccgcagaccu ucgccauccu ggagaacauc     2640

auggccaccu accaggacgc cgagaacccc uuccucgcgu acaagaagga guacggggug     2700

auccggaagu acagcaagaa gggcgggggc ucccccguga ccagccugaa guucaucgac     2760

aagguccugg gccagaagau cgaccucacc gccaagaacc aggccgcccu gcccaagaac     2820

aagaaggugg ugcugcaguc caucacgccg uaccgcgccg acgucuacua cgacgagauc     2880

gacaagcggu accaccucau cggggugaag uacagccugu ucuccuucag caagcagggc     2940

uacgugauca accccaucgc guaccagaag cugcucgaga aggagcagau ccacagcgag     3000

augaaguucu gcuucucccu guaccgcgag gacaucaucg aguucgggga ggaccccgac     3060

agccugaccc gguaccgcuu cugguccaag aacgagagcg gcaagaacaa gaucgagguc     3120

aagcccaucg agaagccgaa cuucgagaag cagaccgugc ucaccaucac gaagaagauc     3180

aagauccuga acaaguacca gaccgacgug cuggggaacu ccuucaaggc ccaggaggag     3240

aaggaccccc ggaacaucga guga                                            3264


<210>  1570
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1570
augcgcuaca agaucggccu ggacaucggg aucaccagcg ugggcugggc cgucaugaac       60

cucgacaucc cccggaucga ggaccugggg gugcgcaucu ucgaccgggc cgagaacccg      120

cagaccggcg agucccuggc gcucccccgc cggcuggccc gcagcgcccg gcgccggcug      180

cgccggcgca agcaccggcu cgagcgcauc cggcgccugg ugauccggga ggggauccug      240

acgaaggagg agcucgacaa gcuguucgag gagaagcacg agaucgacgu cuggcagcug      300

cgcguggagg cccucgaccg gaagcugaac aacgacgagc uggcccgcgu gcuccugcac      360

cuggcgaagc ggcgcggcuu caaguccaac cgcaagagcg agcgguccaa caaggagaac      420

agcaccaugc ucaagcacau cgaggagaac cgcgccaucc uguccagcua ccggaccguc      480

ggcgagauga ucgugaagga ccccaaguuc gcccugcaca agcgcaacaa gggggagaac      540

uacaccaaca cgaucgcccg ggacgaccuc gagcgcgaga uccggcugau cuucuccaag      600

cagcgcgagu ucggcaacau gagcugcacc gaggaguucg agaacgagua caucaccauc      660

ugggcguccc agcggcccgu ggccagcaag gacgacaucg agaagaaggu cggguucugc      720

accuucgagc cgaaggagaa gcgcgccccc aaggccacgu acaccuucca guccuucauc      780

gccugggagc acaucaacaa gcugcggcuc aucagccccu ccggcgcgcg cgggcugacc      840

gacgaggagc ggcgccugcu cuacgagcag gccuuccaga agaacaagau caccuaccac      900

gacauccgga cgcugcugca ccuccccgac gacaccuacu ucaagggcau cguguacgac      960

cgcggcgaga gccggaagca gaacgagaac auccgcuucc uggagcugga cgccuaccac     1020

cagauccgga aggccgugga caaggucuac gggaagggca aguccagcuc cuuccucccg     1080

aucgacuucg acaccuucgg guacgcccug acccuguuca aggacgacgc ggacauccac     1140

agcuaccucc gcaacgagua cgagcagaac ggcaagcgca ugcccaaccu ggccaacaag     1200

guguacgaca acgagcugau cgaggagcuc cugaaccuga gcuucacgaa guucggccac     1260

cucucccuga aggcccugcg gagcauccuc cccuacaugg agcaggggga gguguacucc     1320

agcgccugcg agcgcgccgg cuacaccuuc accgggccca agaagaagca gaagaccaug     1380

cugcugccga acaucccgcc caucgcgaac cccgucguga ugcgggcccu cacgcaggcc     1440

cgcaaggugg ucaacgccau caucaagaag uacggcuccc ccgugagcau ccacaucgag     1500

cuggcccggg accuguccca gaccuucgac gagcgccgga agaccaagaa ggagcaggac     1560

gagaaccgca agaagaacga gaccgcgauc cggcagcuga uggaguacgg gcucacgcug     1620

aaccccaccg gccacgacau cgugaaguuc aagcugugga gcgagcagaa cggccgcugc     1680

gccuacuccc uccagcccau cgagaucgag cggcugcugg agcccgggua cgucgaggug     1740

gaccacguga ucccguacag ccgcucccuc gacgacagcu acaccaacaa gguccuggug     1800

cugacccggg agaaccgcga gaagggcaac cggauccccg ccgaguaccu cggggucggc     1860

acggagcgcu ggcagcaguu cgagaccuuc gugcugacca acaagcaguu cuccaagaag     1920

aagcgggacc gccugcuccg gcugcacuac gacgagaacg aggagaccga guucaagaac     1980

cgcaaccuga acgacacgcg guacaucagc cgcuucuucg ccaacuucau ccgcgagcac     2040

cucaaguucg cggaguccga cgacaagcag aagguguaca ccgucaacgg gcgggugacc     2100

gcccaccucc gcagccggug ggaguucaac aagaaccgcg aggaguccga ccugcaccac     2160

gccguggacg ccgccaucgu cgcgugcacc acgcccagcg acaucgccaa ggugaccgcc     2220

uucuaccagc ggcgcgagca gaacaaggag cuggccaaga agaccgagcc ccacuucccg     2280

cagcccuggc cccacuucgc cgacgagcug cgggcgcgcc uguccaagca ccccaaggag     2340

agcaucaagg cccucaaccu gggcaacuac gacgaccaga agcuggagag ccuccagccg     2400

guguucgucu cccggaugcc caagcgcagc gugaccggcg ccgcccacca ggagacccug     2460

cggcgcuacg uggggaucga cgagcggucc ggcaagaucc agacggucgu gaagaccaag     2520

cugagcgaga ucaagcucga cgccuccggg cacuucccca uguacggcaa ggagagcgac     2580

ccgcgcaccu acgaggcgau ccggcagcgc cugcuggagc acaacaacga ccccaagaag     2640

gccuuccagg agccccucua caagcccaag aagaacgggg agccgggccc cgugauccgg     2700

accgucaaga ucaucgacac gaagaaccag gugauccccc ugaacgacgg caagaccgug     2760

gccuacaacu ccaacaucgu ccgcguggac guguucgaga aggacgggaa guacuacugc     2820

guccccgugu acaccaugga caucaugaag ggcauccugc cgaacaaggc caucgagccc     2880

aacaagcccu acagcgagug gaaggagaug accgaggacu acacguuccg guucucccuc     2940

uaccccaacg accugauccg caucgagcug ccgcgcgaga agaccgugaa gaccgcggcc     3000

ggggaggaga ucaacgucaa ggacguguuc guguacuaca agaccaucga cagcgccaac     3060

ggcggccucg agcugaucuc ccacgaccac cgguucagcc ugcgcggggu cggcucccgg     3120

acgcucaagc gcuucgagaa guaccaggug gacgugcugg ggaacaucua caagguccgg     3180

ggcgagaagc gcguggggcu ggccagcucc gcccacagca agaccggcga gaccgugcgg     3240

ccccuccagu ccacccgcga cuga                                            3264


<210>  1571
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1571
augcgcuaca agaucggccu ggacaucggg aucaccagcg ugggcugggc cgucaugaac       60

cucgacaucc cccggaucga ggaccugggg gugcgcaucu ucgaccgggc cgagaacccg      120

cagaccggcg agucccuggc gcucccccgc cggcuggccc gcagcgcccg gcgccggcug      180

cgccggcgca agcaccggcu cgagcgcauc cggcgccugg ugauccggga ggggauccug      240

acgaaggagg agcucgacaa gcuguucgag gagaagcacg agaucgacgu cuggcagcug      300

cgcguggagg cccucgaccg gaagcugaac aacgacgagc uggcccgcgu gcuccugcac      360

cuggcgaagc ggcgcggcuu caaguccaac cgcaagagcg agcgguccaa caaggagaac      420

agcaccaugc ucaagcacau cgaggagaac cgcgccaucc uguccagcua ccggaccguc      480

ggcgagauga ucgugaagga ccccaaguuc gcccugcaca agcgcaacaa gggggagaac      540

uacaccaaca cgaucgcccg ggacgaccuc gagcgcgaga uccggcugau cuucuccaag      600

cagcgcgagu ucggcaacau gagcugcacc gaggaguucg agaacgagua caucaccauc      660

ugggcguccc agcggcccgu ggccagcaag gacgacaucg agaagaaggu cggguucugc      720

accuucgagc cgaaggagaa gcgcgccccc aaggccacgu acaccuucca guccuucauc      780

gccugggagc acaucaacaa gcugcggcuc aucagccccu ccggcgcgcg cgggcugacc      840

gacgaggagc ggcgccugcu cuacgagcag gccuuccaga agaacaagau caccuaccac      900

gacauccgga cgcugcugca ccuccccgac gacaccuacu ucaagggcau cguguacgac      960

cgcggcgaga gccggaagca gaacgagaac auccgcuucc uggagcugga cgccuaccac     1020

cagauccgga aggccgugga caaggucuac gggaagggca aguccagcuc cuuccucccg     1080

aucgacuucg acaccuucgg guacgcccug acccuguuca aggacgacgc ggacauccac     1140

agcuaccucc gcaacgagua cgagcagaac ggcaagcgca ugcccaaccu ggccaacaag     1200

guguacgaca acgagcugau cgaggagcuc cugaaccuga gcuucacgaa guucggccac     1260

cucucccuga aggcccugcg gagcauccuc cccuacaugg agcaggggga gguguacucc     1320

agcgccugcg agcgcgccgg cuacaccuuc accgggccca agaagaagca gaagaccaug     1380

cugcugccga acaucccgcc caucgcgaac cccgucguga ugcgggcccu cacgcaggcc     1440

cgcaaggugg ucaacgccau caucaagaag uacggcuccc ccgugagcau ccacaucgag     1500

cuggcccggg accuguccca gaccuucgac gagcgccgga agaccaagaa ggagcaggac     1560

gagaaccgca agaagaacga gaccgcgauc cggcagcuga uggaguacgg gcucacgcug     1620

aaccccaccg gccacgacau cgugaaguuc aagcugugga gcgagcagaa cggccgcugc     1680

gccuacuccc uccagcccau cgagaucgag cggcugcugg agcccgggua cgucgaggug     1740

gaccacguga ucccguacag ccgcucccuc gacgacagcu acaccaacaa gguccuggug     1800

cugacccggg agaaccgcga gaagggcaac cggauccccg ccgaguaccu cggggucggc     1860

acggagcgcu ggcagcaguu cgagaccuuc gugcugacca acaagcaguu cuccaagaag     1920

aagcgggacc gccugcuccg gcugcacuac gacgagaacg aggagaccga guucaagaac     1980

cgcaaccuga acgacacgcg guacaucagc cgcuucuucg ccaacuucau ccgcgagcac     2040

cucaaguucg cggaguccga cgacaagcag aagguguaca ccgucaacgg gcgggugacc     2100

gcccaccucc gcagccggug ggaguucaac aagaaccgcg aggaguccga ccugcaccac     2160

gccguggacg ccgucaucgu ggccugcacc acgcccagcg acaucgcgaa ggugaccgcc     2220

uucuaccagc ggcgcgagca gaacaaggag cuggccaaga agaccgagcc ccacuucccg     2280

cagcccuggc cccacuucgc cgacgagcug cgggcccgcc uguccaagca ccccaaggag     2340

agcaucaagg cgcucaaccu gggcaacuac gacgaccaga agcuggagag ccuccagccg     2400

gucuucgugu cccggaugcc caagcgcagc gugaccggcg ccgcccacca ggagacccug     2460

cggcgcuacg ucgggaucga cgagcggucc ggcaagaucc agacgguggu gaagaccaag     2520

cugagcgaga ucaagcucga cgccuccggg cacuucccca uguacggcaa ggagagcgac     2580

ccgcgcaccu acgaggccau ccggcagcgc cugcuggagc acaacaacga ccccaagaag     2640

gcguuccagg agccccucua caagcccaag aagaacgggg agccgggccc cgucauccgg     2700

accgugaaga ucaucgacac gaagaaccag gugauccccc ugaacgacgg caagaccguc     2760

gccuacaacu ccaacaucgu gcgcguggac gucuucgaga aggacgggaa guacuacugc     2820

gugcccgugu acaccaugga caucaugaag ggcauccugc cgaacaaggc caucgagccc     2880

aacaagcccu acagcgagug gaaggagaug accgaggacu acacguuccg guucucccuc     2940

uaccccaacg accugauccg caucgagcug ccgcgcgaga agaccgucaa gaccgccgcg     3000

ggggaggaga ucaacgugaa ggacguguuc gucuacuaca agaccaucga cagcgccaac     3060

ggcggccucg agcugaucuc ccacgaccac cgguucagcc ugcgcggggu gggcucccgg     3120

acgcucaagc gcuucgagaa guaccaggug gacguccugg ggaacaucua caaggugcgg     3180

ggcgagaagc gcguggggcu ggccagcucc gcccacagca agcccggcaa gaccauccgg     3240

ccccuccagu ccacccgcga cuga                                            3264


<210>  1572
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1572
augcgcuaca agaucggccu ggacaucggg aucaccagcg ugggcugggc cgucaucaac       60

cucgacaucc cccggaucga ggaccugggg gugcgcaucu ucgaccgggc cgagaacccg      120

cagaccggcg agucccuggc gcucccccgc cggcuggccc gcagcgcccg gcgccggcug      180

cgccggcgca agcaccggcu cgagcgcauc cggcgccuga ucauccggga ggggauccug      240

acgaaggagg agcucgacaa gcuguucgag gagaagcacg agaucgacgu guggcagcug      300

cgcgucgagg cccucgaccg gaagcugaac aacgacgagc uggcccgcgu gcuccugcac      360

cuggcgaagc ggcgcggcuu caaguccaac cgcaagagcg agcgguccaa caaggagaac      420

agcaccaugc ucaagcacau cgaggagaac cgcgccaucc uguccggcua ccggaccgug      480

ggggagauga ucgucaagga ccccaaguuc gcccugcaca agcgcaacaa gggcgagaac      540

uacaccaaca cgaucgcccg ggacgaccuc gagcacgaga uccgccugau cuucagcaag      600

cagcgggagu ucgggaacau guccugcacc gagaaguucg agaacgagua caucaccauc      660

ugggcgagcc agcgccccgu ggccuccaag gacgacaucg agaagaaggu gggcuucugc      720

accuucgagc cgaaggagaa gcgggccccc aaggccacgu acaccuucca gagcuucauc      780

gccugggagc acaucaacaa gcugcgccuc aucuuccccu ccggggcgcg gggccugacc      840

gacgaggagc gccggcugcu cuacgagcag gccuuccaga agaacaagau caccuaccac      900

gacauccgca cgcugcugca ccuccccgac gacaccuacu ucaagggcau cgucuacgac      960

cgcggggaga gccggaagca gaacgagaac auccgguucc uggagcugga cgccuaccac     1020

cagauccgca aggccgugga caagguguac ggcaagggga aguccagcuc cuuccucccg     1080

aucgacuucg acaccuucgg cuacgcccug acccuguuca aggacgacgc ggacauccac     1140

agcuaccucc ggaacgagua cgagcagaac ggcaagcgca ugcccaaccu ggccaacaag     1200

gucuacgaca acgagcugau cgaggagcuc cugaaccugu ccuucacgaa guucgggcac     1260

cucagccuga aggcccugcg cagcauccuc cccuacaugg agcagggcga gguguacucc     1320

agcgccugcg agcgggccgg guacaccuuc accggcccca agaagaagca gaagaccaug     1380

cugcugccga acaucccgcc caucgcgaac cccgugguca ugcgcgcccu cacgcaggcc     1440

cggaaggugg ugaacgccau caucaagaag uacggguccc ccgucagcau ccacaucgag     1500

cuggcccgcg accuguccca gaccuucgac gagcggcgca agaccaagaa ggagcaggac     1560

gagaaccgga agaagaacga gaccgcgauc cgccagcuga uggaguacgg ccucacgcug     1620

aaccccaccg gccacgacau cgugaaguuc aagcugugga gcgagcagaa cgggcggugc     1680

gccuacuccc uccagcccau cgagaucgag cgccugcugg agcccggcua cguggagguc     1740

gaccacguga ucccguacag ccggucccuc gacgacagcu acaccaacaa ggugcugguc     1800

cugacccgcg agaaccggga gaaggggaac cgcauccccg ccgaguaccu cggcgugggg     1860

acggagcggu ggcagcaguu cgagaccuuc guccugacca acaagcaguu cuccaagaag     1920

aagcgcgacc ggcugcuccg ccugcacuac gacgagaacg aggagaccga guucaagaac     1980

cggaaccuga acgacacgcg cuacaucagc cgguucuucg ccaacuucau ccgcgagcac     2040

cucaaguucg cggaguccga cgacaagcag aagguguaca ccgugaacgg ccgcgucacc     2100

agccaccucc ggucccgcug ggacuucaac aagaaccggg aggagagcga ccugcaccac     2160

gccguggacg ccgccaucgu ggccugcacc acgcccuccg acaucgcgaa ggucaccgcc     2220

uucuaccagc gccgggagca gaacaaggag cuggccaaga agaccgagcc ccacuucccg     2280

cagcccuggc cccacuucgc cgacgagcug cgcgcccggc ugagcaagca ccccaaggag     2340

uccaucaagg cgcucaaccu gggcaacuac gacgaccaga agcuggagag ccuccagccg     2400

guguucguga gccgcaugcc caagcggucc gucaccgggg ccgcccacca ggagacccug     2460

cgccgguaca ucggcaucga cgagcgcagc gggaagaucc agacgguggu gaagaccaag     2520

cuguccgaga ucaagcucga cgccagcggc cacuucccca uguacgggaa ggaguccgac     2580

ccgcggaccu acgaggccau ccgccagcgg cugcuggagc acaacaacga ccccaagaag     2640

gcguuccagg agccccucua caagcccaag aagaacggcg agccgggccc caucauccgc     2700

accgucaaga ucaucgacac gaagaaccag gugauccccc ugaacgacgg gaagaccgug     2760

gccuacaaca gcaacaucgu ccggguggac guguucgaga aggacggcaa guacuacugc     2820

guccccgugu acaccaugga caucaugaag gggauccugc cgaacaaggc caucgagccc     2880

aacaagcccu acuccgagug gaaggagaug accgaggacu acacguuccg cuucagccuc     2940

uaccccaacg accugauccg gaucgagcug ccgcgcgaga agaccgugaa gaccaccacg     3000

ggcgaggaga ucaacgucaa ggacguguuc guguacuaca agaccaucga cuccgccacc     3060

ggcgggcucg agcugaucag ccacgacaac cgcuucuccc ugcggggcgu cgggagccgc     3120

acccucaagc gguucgagaa guaccaggug gacgugcugg gcaacaucua caagguccgc     3180

ggggagaagc gggugggccu ggcguccagc gcccacucca agacgggcga gaccauccgc     3240

ccccuccaga gcacccggga cuga                                            3264


<210>  1573
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1573
augcgcuaca agaucggccu ggacaucggg aucaccagcg ugggcugggc cgucaucaac       60

cucgacaucc cccggaucga ggaccugggg gugcgcaucu ucgaccgggc cgagaacccg      120

cagaccggcg agucccuggc gcucccccgc cggcuggccc gcagcgcccg gcgccggcug      180

cgccggcgca agcaccggcu cgagcgcauc cggcgccugc ugauccggga gggggugcuc      240

acgaaggagg agcuggacaa gcuguucgag gagaagcacg agaucgacgu cuggcagcuc      300

cgcguggagg cccuggaccg gaagcugaac aacgacgagc ucgugcgcgu ccugcugcac      360

cucgccaagc ggcgcggcuu caaguccaac cgccagagcg agcgguccaa caaggagaac      420

agcaccaugc ugaagcacau cgaggagaac cgcgcgaucc uguccggcua ccggaccgug      480

ggggagauga ucgugaagga ccccaaguuc gcccuccaca agcgcaacaa gggcgagaac      540

uacaccaaca cgaucgcccg ggacgaccug gagcgcgaga uccggcugau cuucagcaag      600

cagcgcgagu ucgggaacau guccugcacc gaggaguucg agaacgagua caucgccauc      660

ugggcgagcc agcggcccgu cgccuccaag gacgacaucg agaagaaggu gggcuucugc      720

accuucgagc cgaaggagaa gcgcgccccc aaggccaccu acacguucca gagcuucauc      780

gccugggagc acaucaacaa gcuccggcug aucuccccca gcgggacccg cggccugacc      840

gacgaggagc ggcgccuccu guacgagcag gcguuccaga agaacaagau caccuaccac      900

gacauccgga cgcugcucca ccuccccgac gacaccuacu ucaagggcau cguguacgac      960

uggggcgagu cccugaagca gaacgagaac auccgcuucc uggagcugga cgccuaccac     1020

cagauccgga aggccaucga caaggucuac gggaagggca agagcuccag cuuccugccg     1080

aucgacuucg acaccuucgg guacgcccuc acccuguuca aggacgacgc cgacauccgc     1140

uccuaccugc ggaacgagua cgagcagaac ggcaagcgca ucgcgaaccu cgccaacaag     1200

guguacgaca acgagcugau cgaggagcug cucaaccuga gcuucacgaa guucgggcac     1260

cugagccuca aggcccugcg caacauccug cccuacaugg agcagggcga gguguacucc     1320

agcgccugcg agaaggccgg guacaccuuc accggcccca agaagaagca gaagaccguc     1380

cuccugccca cgaucccgcc caucgugaac cccgugguca ugcgggcgcu gacccaggcc     1440

cgcaaggugg ugaacgccau caucaagaag uacggguccc ccgucagcau ccacaucgag     1500

cuggcccggg accucuccca gaccuucgac gagcgccgga agaccaagcg cgagcaggac     1560

gagaaccgga agcgcaacga gacggccauc cggcagcuga uggaguacgg ccugacccuc     1620

aacccgaccg gccuggacau cgugaaguuc aagcugugga gcgagcagaa cgggcgcugc     1680

gcguacuccc uccagcccau cgagaucgag cggcugcugg agcccggcua cguggagguc     1740

gaccacguga uccccuacag ccgcucccuc gacgacagcu acaccaacaa gguccuggug     1800

cugacgaagg agaaccggga gaaggggaac cgcaucccgg ccgaguaccu cggcgugggg     1860

accgagcggu ggcagcaguu cgagaccuuc guccugacca acaagcaguu cuccaagaag     1920

aagcgcgacc ggcugcuccg ccugcacuac gacgagaacg aggagaugga guucaagagc     1980

cggaaccuga acgacacgcg cuacaucucc cgguucuucg ccaacuucau ccgcgagcac     2040

cucaaguucg ccgagagcga cgacaagcag aagguguaca ccgugaacgg ccgcgucacc     2100

ucccaccucc ggagccgcug ggacuucaac aagaaccggg aggaguccga ccugcaccac     2160

gcgguggacg ccgccaucgu ggccugcacc acgcccagcg acaucgccaa ggucaccgcg     2220

uucuaccagc gccgggagca gaacaaggag cuggccaaga agaccgagcc ccaguucccc     2280

cagccguggc cccacuucgc cgacgagcug cgcgcccggc uguccaagca ccccaaggag     2340

agcaucaagg cccucaaccu gggcaacuac gacgaccaga agcuggagag ccuccagccc     2400

guguucgugu cccgcaugcc gaagcggagc gucaccgggg cggcccacca ggagacccug     2460

cgccgguaca ucggcaucga cgagcgcucc gggaagaucc agacgguggu gaagaccaag     2520

cugagcgaga ucaagcucga cgccuccggc cacuucccca uguacgggaa ggagagcgac     2580

ccccggaccu acgaggccau ccgccagcgg cugcuggagc acaacaacga cccgaagaag     2640

gccuuccagg agccccucua caagcccaag aagaacggcg agcccggccc gaucauccgc     2700

accgucaaga ucaucgacac gaagaaccag gugauccccc ugaacgacgg gaagaccgug     2760

gcguacaacu ccaacaucgu ccggaucgac guguucgaga aggacggcaa guacuacugc     2820

gugcccgucu acaccaugga caucaugaag ggggugcugc ccaacaaggc caucgagccg     2880

aacaagcccu acagcgagug gaaggagaug accgaggacu acacguuccg cuucucccuc     2940

uaccccaacg accugauccg gaucgagcug ccccgcgaga agaccgugaa gaccgccgcc     3000

ggcgaggaga ucaacaucaa ggacauguuc gucuacuaca agaccaucga cagcgcgaac     3060

ggcgggcucg agcugaucuc ccacgacaac agcuucuccc ugcgcggcgu ggggagccgg     3120

acgcucaagc gcuucgagaa guaccaggug gacguccugg gcaacaucua caaggugcgc     3180

ggggagaagc gggugggccu ggccuccagc gcccacucca agaccggcga gaccauccgg     3240

ccgcuccaga gcacccgcga cuga                                            3264


<210>  1574
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1574
augcccaagg aguucgaccc gcugaagccc gccaaguacc gccucgcccu ggacaucggc       60

agcaagucca ucggguucgc gaucaucuuc cugaaggagg acgugaacaa gcuccugaag      120

cccuacgcca ucguccggag cggcgugcgc aucuucgggg acggccggac ccccgccgcc      180

ccggggcagc ugggcuccag ccucgccgcg gagcgccggg ugaagcgcuc cauccggcgc      240

caguacgccc ggaagcgcaa gcggcugcag cgccugcucc ggaaccugaa ggagcugggc      300

uucuuccccg acgacagcaa gcucugcaag uccuucgagg accugaaccc cuacgagcug      360

cgccacaagg ggcucaccaa ggagcugaag ccccacgagu ucggccggcu gcucaugcac      420

auggccaaga accgcggguu ccagagcaac cggaaggucg accugcuggc cgacaacauc      480

gacucccagg cgaaggccga gacgagcgcc augaaguccg ccaucgccac ccuccagagc      540

cgccugaacg gcgaguucga gacccugggg gcguaccucu acgagcgguu caagaagggc      600

gagaccauca aguccagcaa gacgcagaag ggccagaagg ccgaguacga guucuccauc      660

agccgccgga uggugaagca ggaguucgac cgccuguggg ccgcccagga gcaguucaac      720

ccggagcugu ucaacgagca ggccaagaac acccucaagg acaccaucuu cuuccagcgc      780

aaccugcugc cccagaaggu ggggaagugc accuuccucc ccgagcacga gcgggcgucc      840

aaggcccugc ccagcuccca guacuuccgc aucaucagcg agcugaacaa gaucagcuuc      900

acggacgccg agggccucau ccugccgauc ucccuggagc agcggaacaa gcuccucaag      960

agccugcgca ucaagcagaa gcuguccuuc gaccggaucc gcaccgagcu gaaccuggac     1020

agcaccgucc ucaucaaccu ggagcacgcc uccaaggaca gcaucgaggg ggacaagacc     1080

ggcuaccugc ucuccaagaa ggaguacuuc ggccccgccu ggcgggaccu gagccuggag     1140

acgcaggacg cgaucgugac cgcccuccug acccacgaga acgaggccga gcuggugcag     1200

aagcuggucg acgacuucaa caucgcccac gacguggcca aggcgcucgc caacgcccgc     1260

cuggaguccc aguacaugcg gcugagcaag gaggccaucg agcgccucau ccccgagcug     1320

gagaaggggg uggacuccca ggacggcuac aagguccccg ugaaguacca cgaggcgacc     1380

gaggccgccg gguucggcca ccacagcgcc cugcaggaga acugggccga cuucccgacg     1440

cucgaccuga agcagcugca cuccgacgac aacgacaagu acaccguggc ggagcucccc     1500

cugcccuacu acgggaaggu caucacccgc cagguggccu ucggcaccgg cgacgugaac     1560

gaccccgucg agaagcgguu cgggaagauc gccaacccga cggugcacau cugccugaac     1620

caggugcggg uccuccugga cgagaucauc gccaaguacg gcuaccccua cgaggugaac     1680

aucgagcugg cccgcgagcu caagcagaac cacgagcagc ggcagcgcag caucaagcag     1740

caggcggcca acaagaagga gaacgaggag auccggcgcg agaucaagca ggccuacaag     1800

guggagaagg cccggaucau caccgacucc gagaucagcc gcgccgacau cgagaagugg     1860

cgccuguggc ggcgcuccaa gagcaagcug ugcgucuacu ccgggaagaa gaucugcgag     1920

accaugaucc ucaacaacga gguggagguc gaccacaucc ugccccggag ccgcaccggc     1980

gacaacagcu accagaacaa ggugcuggug cucaacaagg cgaaccggau caaguccaac     2040

cgcacgcccu gggaggccaa ggagcuguuc gaggcccagg gguggagcua cgaggacauc     2100

cugaaccgga ugcgcgcccu cgacaagcac cagcuguucc gguucgcgga gaacuccaug     2160

gacaucuggg acaagaacua ccgcagcuuc gaggcccggg cccugaacga cacccgcuac     2220

aucucccggg ucgugaagga cuaccucaag guggucugcc acaaggaccg caucguggug     2280

agcaacggcg cccacaccgc ccugauccgg cgcgaguucg gccugaacga ccuccugggg     2340

aagaagggcg agaagaaccg ggacgaccac cgccaccacg cgaucgacgc cugcgucauc     2400

ggggcccugg accgguccau gcuccgcaag cugcagaucg ccauggaggu ggccgagaag     2460

cagaacagcg cgcggaacuu cgugggccug caggagccgu gguucaacuu cuccacccag     2520

guccgccgca gcaucgggca gaucuacgug ucccacaagc ccaaccacaa cuacgagggc     2580

gagaucuucg aggccagcau cuacggccuc cggcugaucu ccgacaagcg cacggagaag     2640

uacgugggga ucaagcggaa ccccgagacc ggcaagaccg acaagcccaa gaaguccguc     2700

guggugauca gcgacccgaa gacccagaac cgcuacgggc ugcggcacaa guuccacagc     2760

gagggccucc ccaagaagua cuacggccug uccagcagcu ccgucuucug ccuggagauc     2820

uaccgcgacg agaaggggaa cuggcaggcc uacacgaucc cccgguacga ggccuaccgc     2880

aucgugaagc ggcgcggcgu gaacgcccuc uacgacaaca agcggagcaa cuucgagggg     2940

cgcccgcugg ucaugcggcu gaugcgcgac gacugcaucg cguucgagaa gaacggcgug     3000

aaggaggugu accggaucca gaagaucaac gggcucaaga agaacaucac ccugacccgc     3060

cacuucgacg ccaacgucga cgaccgguac cgccggaugc ugaaggccca gaaggccgag     3120

aaggcgggcg aggacuucga cuccgaggac cucugcgacg accugguguu cagcuccuuc     3180

ggcgccagcu cccuguacag caacaaggcc cgcaagguga ccaucucccc cauggggguc     3240

cuccgcgacc ccggcuucaa gggguga                                         3267


<210>  1575
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1575
augaagaagc uggugcucgg ccuggacauc ggggucgcca gcgugggcug ggggaucauc       60

gacgccgaga acggcgacgu gcuggacgcg gggguccgcc ucuucuccag ccgggacgcc      120

uccgagaacg agagccgccg gaccucccgc cggcagagcc gccggcuguc ccgccggaag      180

aagcaccgcc gggagcgcau caagcagcug cuccuggccc acgacaugga cgugugcgac      240

aucgugaacg agaaccccua ccagcugcgg gucaagggcc ucaacgagaa gcuggagauc      300

agcgagaucu acguggcccu gacccaccuc gccaagcgcc ggggcguguc cuaccuggac      360

gacgucgugg aggagggcgg ggcgaucaag agcgagcacc ugaacaagaa cauggaggag      420

aucaaguccg ggaagaugcc gugcgagauc cagcucgagc gcuucgagaa guacggccag      480

gugcggggga ugaucgagau caaccaggac acggaggaga gcuccuuccu ggucaacauc      540

uuccgcacca gcgacuacau caaggaggcc aagaccaucc uggccaccca gcagaaguuc      600

cacaagcaga ucacgaacga guucaucgac gaguacauca acaucauggc cuccaagcgg      660

gacuacuuca ucggccccgg caacgagaag ucccgcaccg acuacggggu guaccggacc      720

aacggcgaca ccugggacaa ccucuucgag auccugaucg ggaagugcag cguguucccc      780

gaggagacgc gcgcggcccg cagcuccuac gucgcccagg aguucaaccu gcucaacgac      840

cugaacaacc ugaagaucag cagccucgag uccggcaagc ugagcaccuc ccagaagcac      900

gacaucgugg agaaggugaa gaacagcaag aaguucggca ccaaggaccu gcucaaguac      960

aucgccuccg ucugcgagug caagaucgag aacaucgacg ggugccggaa ggacaagaac     1020

cgcaagccca ucaugcacac cuucgagaac uaccggaagg ugcacaacgc ccugaacgag     1080

gcgggcgugg accugcgccu cagcgaggcc gacuacgacg agcugaugaa gaucaugacg     1140

cugaacuccg agacccucga gaucaagaag cagugcaagg agaagcugcc gcaccugacc     1200

gaggacuacc ucaacgucug ccuggugcug aacaagaagg gggccuucag cggcuggcac     1260

gcccucuccc ugaaggccau gaagcugaug augcccgagc ucuacgggac cagcaagaac     1320

cagaugcaga ucauccacac gaugcagcug ggcaaggcgu acaucaagcc ccugaaccag     1380

cagaaguacc ucaacuccga ggccauccug gaggagaucu acaaccccau cgccgcccgg     1440

agcauccgcc agaccaucaa ggugaccaac gccauccuga agaaguacgg cccgcucgac     1500

uccgucauca ucgagaugcc ccgggacuac cagaccagcg aggacgagcg caagaugauc     1560

gagaagaugc agaaggagaa cgagaaggag aagaaggcgg cccugaagcg ggcccgcgag     1620

gaguaccccu uccccgacga ggccuuccgg aaccacaagg agcuggccac gaagcugcgc     1680

cucugguacc agcaggacca guucugcgug uacuccgggc ggaagaucag cgucguggac     1740

cuggugcgca acccgaacca guucgagauc gaccacauca ucccccaguc cgucagccug     1800

gacgacuccc ucaacaacaa ggugcugugc uucagcgagg agaaccagaa gaagggccag     1860

cagacccccu ucuacuacca caagggcaag agcgggaacu ggaccuacga gcaguacaag     1920

cuguucgugg agaagcuguu cgacaagaag aagaucucca acaagaagcg ggagcugcuc     1980

cucuucgagg aggacaucaa caagugggac guccgccggg gguucaucgc gcgcaaccug     2040

guggacaccc gguacgccag ccgcgugauc cugaacuccu accagcagua cuucggcaac     2100

cacgagaugg gcacgaaggu caagguggug cgcgggaagu ucaccagcca gcuccggaag     2160

aaguggaaga ucuucaagga ccgcaacgag ucccacagcc accacgccau cgacgcccug     2220

aucgucgcgg ccaccgccaa ccugcggcuc uggaagaaga acaagcuggg caucuccgac     2280

gaggggaagg uguacgacaa ggagaccggc gagauccugc ccuucgagaa gcucacggcc     2340

agcgaguaca acgagaugau gaacaaggag ccguaccuga acuucaagac caagcugcag     2400

aacaucgaga ccaaggugaa guacuccuac aaggucagca agaaggugaa ccgcaagauc     2460

uccaacgaca ccaucuacag cacgcgggag cgcgacggga ucgacuacau cgugggcaag     2520

aucaagaaca ucuacgacgu caagaccuac aaggaccagu uccucaagcg guacaagaag     2580

aacaaggagc aguuccugau guaccagcac gacccccaga ccuucgccau ccuggagucc     2640

aucauggcga ccuaccccga cgccgagaac cccuuccucg ccuacaagaa ggagcacggc     2700

gugauccgca aguacgccaa gaacaaggcc ggggcgccgg ugacgagccu gaaguucauc     2760

gacggcgccc uggggcagaa gaucgacguc accuacaaga acaaggccgc ccuccccaag     2820

aacaagaagg uggugcugca guccaucaag cccuaccggg ccgacgucua cuacgacgag     2880

ggcaacaagc gcuaccaccu gaucggcgug aaguacagcc ucuucuccuu cagcaagcag     2940

ggguacguga ucaaccccau cgcguaccag aagcugcugg agaaggagca gauccacagc     3000

gagaugaagu ucugcuucuc ccucuaccgg gaggacauca ucgagagcgg cgaggacccg     3060

gacucccuga cccgcuaccg guucuggagc aagaacgagu ccgggaagaa caagaucgag     3120

gucaagccca ucgagaagcc caacuucgag gaccgcaagg ugaucaccau caccaagaag     3180

aucaagaucc ugaacaagua ccagacggac gugcucggca acagcuucaa ggcccaggag     3240

gagaaggacc cccgguccau cgaguga                                         3267


<210>  1576
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1576
augaacuaca agaugggccu ggacaucggg aucgccagcg ugggcugggc cgucaucaac       60

cucgaccuga agcgcaucga ggaccugggg gugcggaucu ucgacaaggc ggagcacccc      120

cagaacggcg agucccucgc ccugccgcgc cggaucgccc gcagcgcccg gcgccggcug      180

cgccggcgca agcaccggcu cgagcgcauc cggcgccugc ugguguccga gaacguccuc      240

accaaggagg agaugaaccu gcuguucaag cagaagaagc agaucgacgu guggcagcuc      300

cggguggacg cccuggagcg caagcugaac aacgacgagc ucgcgcgggu ccugcugcac      360

cucgccaagc gccggggguu caagagcaac cgcaaguccg agcggaacag caaggagucc      420

agcgaguucc ugaagaacau cgaggagaac caguccaucc uggcccagua ccgcagcgug      480

ggcgagauga ucgugaagga cuccaaguuc gccuaccaca agcgcaacaa gcucgacagc      540

uacuccaaca ugaucgcgcg ggacgaccug gagcgcgaga ucaagcugau cuucgagaag      600

cagcgggagu ucaacaaccc cgucugcacc gagcgccucg aggagaagua ccugaacauc      660

uggagcuccc agcggcccuu cgccagcaag gaggacaucg agaagaaggu gggcuucugc      720

acguucgagc ccaaggagaa gcgcgccccg aaggccaccu acaccuucca guccuucauc      780

gugugggagc acaucaacaa gcugcggcuc gucagccccg acgagacccg cgcccugacg      840

gagaucgagc ggaaccugcu cuacaagcag gcguucucca agaacaagau gaccuacuac      900

gacauccgca agcugcugaa ccucagcgac gacauccacu ucaaggggcu gcuguacgac      960

cccaagagcu cccucaagca gaucgagaac auccgguucc uggagcugga cagcuaccac     1020

aagauccgca agugcaucga gaacguguac ggcaaggacg ggauccggau guucaacgag     1080

accgacaucg acaccuucgg cuacgcccuc acgaucuuca aggacgacga ggacaucgug     1140

gccuaccugc agaacgagua caucaccaag aacgggaagc gcgucuccaa ccuggccaac     1200

aagguguacg acaagagccu caucgacgag cugcugaacc ucuccuucag caaguucgcc     1260

caccugucca ugaaggcgau ccggaacauc cugcccuaca uggagcaggg cgagaucuac     1320

agcaaggccu gcgagcucgc cggcuacaac uucaccgggc cgaagaagaa ggagaaggcc     1380

cugcugcugc ccgugauccc caacaucgcc aaccccgucg ugaugcgcgc gcucacccag     1440

ucccggaagg uggucaacgc caucaucaag aaguacggca gcccgguguc cauccacauc     1500

gagcuggccc gcgaccugag ccacuccuuc gacgagcgga agaagaucca gaaggaccag     1560

acggagaacc gcaagaagaa cgagaccgcc aucaagcagc ucaucgagua cgagcugacc     1620

aagaacccca ccgggcugga caucgugaag uucaagcucu ggagcgagca gcagggccgc     1680

ugcauguacu cccugaagcc caucgagcug gagcggcucc uggagcccgg cuacgucgag     1740

guggaccaca uccugccgua cagccgcucc cucgacgaca gcuacgccaa caagguccug     1800

gugcugacga aggagaaccg ggagaagggg aaccacaccc ccguggagua ccucggccug     1860

ggguccgagc gcuggaagaa guucgagaag uucguccugg cgaacaagca guucagcaag     1920

aagaagaagc agaaccuccu gcggcugcgc uacgaggaga ccgaggagaa ggaguucaag     1980

gagcggaacc ucaacgacac ccgcuacauc agcaaguucu ucgccaacuu caucaaggag     2040

caccugaagu ucgccgacgg cgacgggggc cagaaggugu acacgaucaa cggcaagauc     2100

accgcccacc uccggucccg cugggacuuc aacaagaacc gggaggagag cgaccugcac     2160

cacgcggugg acgccgucau cguggccugc gccacccagg ggaugaucaa gaagaucacc     2220

gaguucuaca aggcccgcga gcagaacaag gaguccgcga agaagaagga gcccaucuuc     2280

ccccagccgu ggccccacuu cgccgacgag cugaaggccc ggcugagcaa guucccccag     2340

gaguccaucg aggccuucgc ccucggcaac uacgaccgca agaagcugga gagccugcgg     2400

cccguguucg ucucccgcau gccgaagcgg agcgugacgg gggcggccca ccaggagacc     2460

cuccgccggu gcgugggcau cgacgagcag uccgggaaga uccagaccgc cgucaagacc     2520

aagcugagcg acaucaagcu ggacaaggac ggccacuucc ccauguacca gaaggagucc     2580

gacccccgca cguacgaggc cauccggcag cgccuccugg agcacaacaa cgaccccaag     2640

aaggccuucc aggagccgcu guacaagccc aagaagaacg gcgagcccgg gccggugauc     2700

cgcaccguga agaucaucga caccaagaac aaggucgugc accucgacgg cagcaagacc     2760

guggcguaca acuccaacau cguccggacg gacguguucg agaaggacgg gaaguacuac     2820

ugcgugcccg ucuacaccau ggacaucaug aagggcaccc ugcccaacaa ggccaucgag     2880

gccaacaagc ccuacagcga guggaaggag augaccgagg aguacacguu ccaguucucc     2940

cuguucccga acgaccucgu gcgcaucgug cugccccggg agaagaccau caagaccagc     3000

accaacgagg agaucaucau caaggacauc uucgccuacu acaagaccau cgacuccgcg     3060

accgggggcc uggagcucau cagccacgac cgcaacuuca gccugcgggg cgucgggucc     3120

aagacgcuga agcgcuucga gaaguaccag guggacgugc ucggcaacau ccacaagguc     3180

aagggggaga agcggguggg ccuggccgcc cccaccaacc agaagaaggg caagaccgug     3240

gacagccugc aguccgucag cgacuga                                         3267


<210>  1577
<211>  3270
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1577
augaccgccu ucaagcccaa cccccugaac uacauccucg gccuggacau cgggaucgcc       60

agcgugggcu gggcgauggu cgagaucgac gacaaggaga accccgugcg ccugaucgac      120

cucggggugc gggucuucga gcgcgccgag augcccaaga ccggcgacuc ccuggccgcc      180

gcccggcgcc uggcgcggag cgugcgccgg cucacgcgcc ggcgcgccca ccggcugcug      240

cgcgcccggc gccuccugaa gcacgagggg gugcugaagc ccgaggacuu cgacgagaac      300

ggccucguca agccgcugcc cuacaucccc aagcagcccc ggaacacccc guggcagcug      360

cgcgccgcgg cccucgaccg gaagcugacg ccccuggagu gggccgccgu gcuccugcac      420

cuggugaagc accgcggcua ccucucccag cggaagaacg agggggagac cgacgacaag      480

gagcugggcg cccugcucaa gggggucgcg gacaacaccc acgcccugca ggccggcaac      540

uuccgcaccc ccgccgagcu ggcccucaac aaguucgaga aggagagcgg gcacauccgg      600

aaccagcgcg gcgacuacuc ccacaccuuc agccgcaagg accugcaggc ggagcuggug      660

cuccuguucg agaagcagaa ggaguucggc aaccccuacg uguccgacga ccugaagaac      720

gggaucgaga accuccugau gacccagcgg ccggcccuga gcggcgacgc cauccagaag      780

augcucgggc acugcacguu cgagcccgcc gagcccaagg ccgcgaagaa caccuacacc      840

gccgagcgcu ucaucuggcu gaccaagcug aacaaccucc ggauccugaa ccagggcaac      900

gagcgccccc ugacggacgc cgagcggacc acccucaugg acgagccgua caagaagucc      960

aagcugaccu acgcccaggc ccgcaagcug cucgaccugg acgacacggc guucuucaag     1020

ggccugcggu acgggaagga caacgccgag agcgccaccc ucauggagau gaaggccuac     1080

cacgcgaucu cccgcgcccu ggagaaggag ggccugaagg acaagaaguc cccgcugagc     1140

cucagccccg agcugcagga cgagaucggg accgccuucu cccuguucaa gaccgacgag     1200

gagaucacgg gccggcucaa ggggugccug cagcccgaga uccuggacgc ccuccugaag     1260

cacaucagcu ucgacaaguu cguccagauc ucccugaagg cccuccgccg gaucgugccc     1320

cugauggagc agggcaagcg cuacgacgag gcgugcgccg agaucuacgg cgaccaccac     1380

gggcggcgca acagcgagga gacccuguac cucccgccca uccccgccga cgagauccgg     1440

aaccccgugg uccugcgcgc ccugucccag gcccggaagg ugaucaacgg cguggggcgc     1500

cgguacggca gccccgcgcg cauccacauc gagaccgccc gcgaggucgg caagagcuuc     1560

aaggaccgga aggagaucga gaagcgccag gaggagaacc ggaaggaccg cgagaaggcc     1620

gccgccgagu uccaggagua cuuccccaac uucguggggg agcccaaguc caaggacauc     1680

cucaagcugc ggcuguaccg ccagcagaac ugcaagugcc ucuacagcgg caaggagauc     1740

aaccuggggc ggcugaacga gaagggcuac guggagaucg accacgcgcu cccguucucc     1800

cgcaccuggg acgacagcuu caacaacaag guccuggugc ugggguccga gaaccagaac     1860

aagggcaacc ggacgcccua cgaguaccuc aacgggaagg acaacagccg cgaguggcag     1920

gaguucaagg cccgggugga gaccucccgc uucccccaca gcaagaagca gcggauccug     1980

cugcagaagu ucgacgagga gggcuucaag gagcgcaacc ucaacgacac ccgguacguc     2040

aaccgcuucc ugugcaaguu cguggccgac cacaugcagc ugaccggcaa ggggaagcgg     2100

cgcgucuucg ccuccaacgg ccagaucacg aaccuccugc ggggguucug gggccugcgc     2160

aaggugcggg cggagaacga ccgccaccac gcccucgacg ccguggucgu ggccugcagc     2220

accguggcca ugcagcagcg caucacccgg uucguccgcu acaaggagau gaacgcguuc     2280

gacggcaaga ccguggacaa ggagacgggg gaggugcugc accagaagac ccacuucccg     2340

cagcccuggg aguucuucgc ccaggagguc augauccggg uguucggcaa gcccgacggg     2400

aagcccgagc uggaggaggc cgacaccccg gagaagcucc gcgcccugcu ggccgagaag     2460

cucuccagcc ggcccgaggc ggugcacgag uacgucgcgc cccuguucgu gucccgcgcc     2520

cccaaccgga agaugagcgg ccaggggcac auggagacca ucaagucccc gaagcgccug     2580

gacgagggca ucagcguggu ccgggugccc cucacgcagc ugaagcugaa ggaccucgag     2640

aagaugguga accgcgagcg ggagcccaag cuguacgagg cccugaaggc ccgccucgag     2700

gcccacaagg acgaccccgc caaggccuuc gccgagccgu ucuacaagca ggacaaggcg     2760

ggcaaccgga cccagcaggu caaggccgug cgcaucgagc agguccagaa gaccggggug     2820

ugggugcgga accagaacgg caucgccgac aacgccacca uggugcgcgu cgacguguuc     2880

gagaagggcg ggaaguacua ccuggugccc aucuacuccu ggcaggucgc caaggggauc     2940

cugcccgaca aggcgaucau cgccuucaag gacgaggagg aguggcagcu caucgacgac     3000

agcuucaacu ucaaguuccc ccugcacccg aacgaccugg uggaggugau caccaagaag     3060

gcccggaucu ucggcuacuu cgccuccugc caccgcggca cggggaacau caacauccgg     3120

auccacgacc ucgacaacaa ggucggcaag aacgggaucc uggagggcau cggggugaag     3180

accgcccuga gcuuccagaa guaccagauc gacgagcucg gcaaggagau ccgccccugc     3240

cgccugaaga agcggccgcc cgugcgcuga                                      3270


<210>  1578
<211>  3270
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1578
augaacgugc gcuacaagau cggccuggac aucgggauca ccagcgucgg cugggccgug       60

gugaaccucg acaucccccg gaucgaggac cugggggucc gcaucuucga ccgggccgag      120

aacccgcaga ccggcgaguc ccuggcgcuc ccccgccggc uggcccgcag cgcccggcgc      180

cggcugcgcc ggcgcaagca ccggcucgag cgcauccggc gccugcugau ccgggagggg      240

auccucauga aggaggagcu ggacaagcug uucgaggagc gccacgagau cgacgugugg      300

cagcuccggg uggaggcccu ggaccgcaag cugaacuccg acgagcucgc ccggguccug      360

cugcaccucg cgaagcgccg cggcuucaag agcaaccgga aguccgagca caacagccgc      420

gagaacucca cgaugcugaa gcacaucgag gagaaccggg ccauccugag cuccuaccgc      480

accguggccg agaugaucgu gaaggacccc aaguucgccc uccacaagcg gaacaagggc      540

gagcacuaca ccaacaccau cgcgcgcgac gaccuggagc gggagaucca gcugaucuuc      600

acgaagcagc gcgaguucgg gaacaugaac ugcaccaagg aguucgagaa cgaguacauc      660

gccaucuggg ccagccagcg gcccuucgcc uccaaggacg acaucgagaa gaaggucggc      720

uucugcaccu ucgagccgaa ggagaagcgc gcccccaagg cgaccuacac guuccagagc      780

uucaucgccu gggagcacau caacaagcuc cggcuggugu cccccgccgg gacccgcggc      840

cugaccgacg aggagcggcg ccuccuguac gagcaggccu uccagaagaa caagaucacc      900

uaccacgaca uccggacgcu gcuccgccug cccgacgaca ccuacuucaa gggggugguc      960

uacgaccgga acaugccgcu gaagcagaac gagaccaucc gcuuccucga gcuggacgcc     1020

uaccaccaga uccggaaggu gaucgacaag gcguacggca agggcaagag cuccagcuuc     1080

cugcccaucg acuucgacac cuucggguac gcccucacgc uguucaagga cgacgccgac     1140

gugcgcuccu accugcagaa cgaguacgag cagaacggca agcccaugcc caaccucgcc     1200

aacaagaccu acgacaacga gcugaucgag gagcugcuca accugagcuu caccaaguuc     1260

gggcaccugu cccucaaggc ccugcacaac auccugccgu acauggagca gggcgagguc     1320

uacagcagcg cgugcgagcg cgccggcuac aaguucaccg ggccccggaa gaagcagaag     1380

acgaugcucc ugcccaacau ccccuccauc gccaacccgg uggugaugcg cgcccugacc     1440

caggcccgga aggucgugaa cgcgaucauc aagaaguacg gcagccccgu guccauccac     1500

aucgagcugg cccgcgaccu cagccagacc uucgacgagc ggcgcaagac caagcgggag     1560

caggacgaga accgcaagaa gaacgagacg gccauccggc agcugaugga guacgggcug     1620

acccucaacc ccaccggcca cgacaucguc aaguucaagc ugugguccga gcagaacggg     1680

cgcugcgccu acagccugca gcccaucgag aucgagcggc uccuggagcc gggcuacacc     1740

gagguggacc acgugauccc cuacucccgc agccuggacg acuccuacac gaacaagguc     1800

cucgugcuga ccaaggagaa ccgggagaag ggcaaccgca cccccgcgga guaccugggg     1860

gucggcaccg agcgguggca ccaguucgag acguucgugc ucaccaacaa gcaguucagc     1920

aagaagaagc gcgaccggcu gcugcgccuc cacuacgacg agaacgagga gaccgaguuc     1980

aagaaccgga accugaacga cacccgcuac aucucccggu ucuucgccaa cuucauccgc     2040

gagcaccuga aguucgccga gagcgacgac aagcagaagg uguacacggu caacgggcgc     2100

aucaccgccc accuccgguc ccgcugggag uucaacaaga accgggagga gagcgaccug     2160

caccacgccg uggacgcggc caucguggcc ugcaccaccc ccuccgacau cgccaagauc     2220

accgccuucu accagcgccg ggagcagaac aaggagcugg cgaagaagac ggagccgcac     2280

uucccccagc ccuggcccca cuucgccgac gagcuccgcg cccggcugag caagcacccg     2340

aaggagucca ucaaggcccu gaaccucggc aacuacgaca acgagaagcu ggagagccug     2400

caccccgucu ucgugucccg caugcccaag cggagcguga ccggggccgc gcaccaggag     2460

acccuccgcc gguacgucgg caucgacgag cgcagcggca agauccagac cguggugaag     2520

acgaagcugu ccgagaucaa gcuggacgcc agcgggcacu ucccgaugua cggcaaggag     2580

uccgaccccc ggaccuacga ggccauccgc cagcggcucc uggagcacaa caacgacccc     2640

aagaaggccu uccaggagcc ccuguacaag ccgaagaaga acggggagcc cggccccauc     2700

auccgcaccg ucaagaucau cgacaccaag aaccaggugg ugccccucaa cgacgggaag     2760

acggucgcgu acaacagcaa caucgugcgg guggacgucu ucgagaagga cggcaaguac     2820

uacugcgugc cgguguacac cauggacauc augaagggcg uccugcccaa caaggccauc     2880

gagcccaaca agcccuacuc cgaguggaag gagaugaccg aggacuacac cuuccaguuc     2940

agccuguacc cgaacgaccu cauccgcauc gagcugcccc gggagaaggc cauccgcacg     3000

uccaccgggg aggagaucaa gaucaaggac uucuucgccu acuacaagac cauccacagc     3060

ggcaccgggg gcauggagcu gaucucccac gaccgcagcu ucucccucag cggcgugggg     3120

ucccggacgc ugaagcgcuu cgagaaguac cagguggacg uccugggcaa caucuacaag     3180

gugcgcgggg agaagcgggu gggccucgcc agcuccgcgc acagcaagac cgggaagacc     3240

guccggcccc ugcaguccac ccgcgacuga                                      3270


<210>  1579
<211>  3273
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1579
augcgcuggc ggcugggccu cgacaugggg gccggcagcc ugggguggug cgccgugggc       60

cuggacgacc agggguaccc cgacuccauc cucgcggccg gcagccacau cuucggcgac      120

gggcgcgccc cgucccgggg cgaccagcag gggacgcccc ugggcgucga gcgccgggcc      180

gccaaggcga ugcgccggcg ccgggaccgc uuccuggggc ggcaggccaa gcuccuggcc      240

cgccugaccc ucgccggccu guuccccgcg gaccccgugc gggccaaggc ccuggagcuc      300

cuggacccgu acgugcugcg cgcccgggcc cucgacgagg cgcugccgcc ccaccagcug      360

ggccgcgccu ucuuccaccu caaccagcgg cgcggguuca agagcaaccg gaagaccgac      420

cgcggcgucg acgacgaggc cgggaagauc gccaucggca ucgaccggcu gcgcgccgcg      480

aucgccgccg ccgaggcccg caccaugggc gaguuccugc acaagcggcg ccaguccgcg      540

cccgacguga accagauccc cagcgugcgg acccgccucc ggcccgagac cggggaggag      600

gccaagggcg acggguacga cuucuacccc ggccgcgagc uggucgagga ggaguucgac      660

gccaucuggg acgcccaggc gccccaccac ccgucccugc ucacgcccga caaccgggcc      720

cugcugcacg aggccaucuu cuaccagcgc ccccucaagg cccccaagau cgggaagugc      780

acccugcuga acaacgagcc gcggcucccc aaggcccacc cccuguucca gcgccggcgc      840

cugcucgagg aggugaacgc gcugcggauc gugcgccccg gcgaggucgc caccaagcug      900

accugcgagc agcgggaccu ccugaugcug aagcucaagg acaaggccaa ggugagcuuc      960

gagacgcugc gcuccaaggu gcugaagcuc gagccggagg cccgguucaa caaggagggc     1020

gagaaccgca ccgagcuggu cggggacgag gugugcgccc agaugagcgc gaagacccgg     1080

uucggcaagg ccugggccca cuucaccgac gagaagcgcu gggccauccu gucccgccuc     1140

aucgaggagg aggacgagga gcggcuggac acguggcugc gcaccgagca cgggcucgac     1200

gccgagcagg cgcgggccgu ggccggcgcc cgccugcccg ccggguucgg ccgguucggc     1260

gagaccgcga cccgccggcu gaucgccgcc cugcaggccg aggucaucgu guacagcgag     1320

gcgguggucg ccgccgggcu cggccaccac uccgacaugc gcacggggga ggugcuggcc     1380

gagcugcccu acuacggcau gacccucgag cggcaccucc ugcccgggac cggcgacaag     1440

gccgacccgg aggagcuccg caucggccgg cugaccaacc ccacggugca caucgcgcug     1500

aaccgccucc ggcgcaucgu caaccggcug aucaagaccu acgggcgccc cgagcagauc     1560

gccaucgagc ugggccggga gcucaagcug accgacgagg acaagcagcg ccugagccgg     1620

cagaacaacc gcaaccgggc cgacgccgag gcccgcucca agaagcucgc ggagcugggg     1680

caggccgaca acggcgccaa ccgcgcccgg cugaagcucu gggaggagcu gaaccgcgag     1740

aacgcccugg accggcgcug caucuacagc ggccagcaga ucuccaucgc gaugcuguuc     1800

agcgacgccg uggaggugga ccacauccug cccuucgacg ccacccugga cgacuccaac     1860

aacaaccgga uccucugccu gcgcgagugg aaccggcgca agcggaagca gagcccguac     1920

gaggccuggg ggcacacgga cuacuggccc gacaucgcgg cccgcgccgc ccggcugccc     1980

aaggagaagc gcuggcgguu cgagccggac gccaugcagc gcuucagcgc ggaggaccgg     2040

uucaucgccc gccaccucgu cgacacccag uacauguccc ggcuggcccg cgaguaccug     2100

gaggcccucu accccgagaa gggcgagggg agcggccacg ugugggucuc ccccgggcgg     2160

cugaccgagc ugauccgccg caagcucggc cugaacgagc ugcuccccga ccacaacuuc     2220

gccggcguga agaaccggcu ggaccaccgc caccacacca ucgacgcguu cguggucgcc     2280

gugacggacc ggagccugcu ccaggggauc ucccgcgcca gcgcccugga gggcgaggag     2340

gggcgggagc gcgugaucgu cccggagccc ugggacggcu uccgggaggc ccugcgcacc     2400

acccucaaca ccaucacggu guggcaccgg caggaccacg ggaccgcguc caaggccggc     2460

cuggcccagg gccgcgacca gaccgccggg aagcugcaca acgacaccgc cuacggccuc     2520

acggggcggc aggacaccaa gggcaacgac cuggugguca gccgcaagcc ccugaugucc     2580

cucaagcccg cggacaucga gccggugccc gacguggccc ccaucaccaa ggggguccgg     2640

gaccgcgacc ugcggaaggc ccuggcccac uucacccagg cgaccgaggg caagggcuuc     2700

cuccaggccc uggagcgcuu cacgcggcug gggccccucc acuuccgcgg cauccggcgc     2760

gugcggcuga uccagccgcu ggccgugauc cccaucaagg ggcccggcgg gguccccuac     2820

aagggcuacc ucggcgggga gaacuuccgc ugcgacgugu gggagaugcc ggacggcgcc     2880

uggaagcccg aggugaccag cauguuccag gcccaccagc ccggguggac cuccgcgauc     2940

cgcgccgccu gccccaccgc ccggaagguc cugagccugc acaagcgcga caugaucgcc     3000

aucgagcggg aggagggcgu gcgcacgcuc augguggucg cgcagaucug guccaacggc     3060

caggugaccc ugguggagcc gcacgaggcc ggggaccuga acaagcggaa caagagcgag     3120

gacgaccccu ucggcuacuu caugcccacc gccgggggcc ucaagcgccu gaaggcccgg     3180

cagguccgcg uggacgagcu ggggcgggug caggaccccg gcuucccggc ccgcaaggcg     3240

cggcgccgga cccgccccaa gcaggucgac uga                                  3273


<210>  1580
<211>  3273
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1580
auggacuggc gccugggccu cgaccugggg gccaacagcc ugggcugggc cgcgcuccgg       60

cuggaccgcg ccggggaccc caucggcauc cuggccaugg ggucccggau cuucagcgac      120

ggccgcgacc cgaaguccgg cgccagccuc gccguggacc ggcgcgcggc ccgggccaug      180

cgccggcgcc gggaccgcuu caagcagcgg caggccgcgc ugcugaagua ccucauccgc      240

gacgggcugu uccccgccga ccaggccgac cggcagcgcc uggccgacga ggaccccuac      300

gcccuccggg cgcgcgcccu ggacgaggag cugucccucu ucgagaucgg ccgggcccug      360

uuccaccuga accagcgccg gggguuccag agcaaccgca aggccgaccg cggccaggac      420

gacgaggccg ggaagaucgc ggucggcgug gaccggcuca accagcagau ggaggagcag      480

ggcgcccgca cccuggggga guuccugcac cagcggcgcc gggccgccac cgacgccaac      540

cacauccccu ccgugcgcac gcggcuccgc ccggagaccg gcgagggggc gaagggcgac      600

ggcuacgacu ucuaccccag ccgggcccug aucgccgagg aguucgagga gaucugggcc      660

gcccaggcgc cccacuaccc cgacacccug acccagcagg uccacgaccg ccucuacgag      720

aucaucuucc accagcggcc gcugcgcgcg ccccaggugg ggcagugcac gcugguggcc      780

ggcgagaccc ggcuccccaa ggcccacccc cuguuccaga agcgccggcu gcucgaggag      840

aucaacgccc ugaugaucgu ccgcccgggg gagaucgccc agcggcugac ccccgaggag      900

cgcgacaccc ucuaccugaa gcugaaggac aagcggacgg uguccuucgc cacccuccgc      960

aagacccuga agcuggacag cgccgcccgc uucaacaagg aguccgagaa ccggaccgag     1020

cucaagggcg acgagguggc ggccgagcug ggggccaaga cgcgccuggg cacccggugg     1080

cagcaccuca gcggcgacca gcagugggag gucgugcacc gccugauggc ccuggagucc     1140

caggccgacg aggcggccuu ccgggacugg aucagccaga ccuacgaccu ccugcccgag     1200

caggccgccg ccgugcuggg ggcgcgccuc ccccagggcc acgggcgguu cggcccgacc     1260

gccacgacca cccugaucga cucccugcug cacgaccgca ccgaggacgg gcgggucgug     1320

guguacagcg aggccgucca gcgcuccggc cucggccacc acagcgaccu gcggcacgac     1380

cgcgacgacg aggacgugac ggagcugccc uacuacgggg uggcccucga gcggcacauc     1440

augcccggca ccgcggagcc caccgacccg gaggagaugc gcaucgggcg gcugaccaac     1500

cccacggucc acaucggccu gaaccagcuc cgccacgugg ugaaccggcu gauccgcgcc     1560

uacgggccgc ccaaggagau cgccaucgag cuggcccggg agcucaagcu guccgaggac     1620

gagaagaaga agcgcaacag cgagaaccgg gacaaccgcc uggccgcgga gggccgcagc     1680

aagaagcucg aggagauccg gcagcgcgac accggcgcca accgggcccu gcugaagcuc     1740

ugggaggagc ugaaccccgg gaacguccug gaccgccggu gcaucuacac cggcgagcag     1800

aucuccaucg acaugcuguu cagcgaccgc guggaggugg accacauccu ccccuucgcc     1860

cagacccugg acgacuccaa cgggaacaag cuccugugcc ugcgggaggc caaccgcgag     1920

aagcggcgcc ggagccccua cgaggcgugg ggccacacgg accgcuggcc cgacaucgcc     1980

gagcgggccu cccgccuccc gaagcccaag cgguggcgcu ucgagcccga cgccaugcag     2040

cgguucgcca ccgaggacgg cuuccuggcg cgccaccugg ucgacaccca guaccucggg     2100

cggcuggccc gcgaguaccu gagcuggcuc uacccgggcc gcggggaggg cuccgggcac     2160

guguggguca gccccggccg gcugaccgag auggugcgcc ggaagcuggg ccucaacgac     2220

cugcugcccg accacaacuu cgggggcggg gccgaccagc ccaagaaccg ccucgaccac     2280

cggcaccacg ccaucgacgc gcuggugguc gccaucgugg accgcggcau gcugcaggcc     2340

aucgcccggg ccuccgggca ggacggcagc gagggccgcg agcggaucca caucccggcg     2400

cccugggacg gguuccgcga cgaccuccgg guggccguca acggcaucau cgugucccac     2460

cgccccgacc acgggacggu ggccaaggcc ggccuggccc gggggcgcga ccagaccgcg     2520

ggccggcugc acaacgacac cgccuacggc cucaccgggg agacggacgc caagggcaac     2580

agcaucgucg ugcgccggau cccccugucc gccaucaaga agccggccga caucgcgcgc     2640

gugcgggacc cccugcuccg caacgcccug gccgaguuca ccaaggggcu ggacggcaag     2700

gccuucgagg cccggaucgg gcgcuuccac gagcucggcc cccugcugua ccgcggcauc     2760

cggcgcgucc ggguggugga gacccucacc gucaucccca uccgcgacgc ggaggggcgg     2820

gccuacaagg gcuacaaggg ggacagcaac uaccgcuacg acguguggga gaugccggac     2880

ggcaaguggc ggugccaggu ggucuccaug uucgacgccc accagagcac cgagcaggcc     2940

cgcccccacc ccgcggcccg gaagauccug ucccugcagc gcaacgacau ccucgccauc     3000

gagcggacgg agggcgaccg cgagcuggug cgggugguca aguucagcga gaagcaguuc     3060

gccguggcgc ccccgcacga gggcgggucc cugaagaccc gcgacgggaa cgccgacgac     3120

cccuuccggu accucuaccc cagccccaac acccugaagg gcuggcgcgc ccgccaggug     3180

cggaucgacg agcuggggcg gguccucgac ccgggcuucc ccgcccgcaa gagcgugcgc     3240

cggacccgcc cccgggccgc ccccguggac uga                                  3273


<210>  1581
<211>  3276
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1581
auguacagca accugcuguu cuccuucgac aucggcacca acagcaucgg guggugcgug       60

uucgcccugg acgaggccgg cgagcccuac cgcaucgucg accugggggc gcggaucuac      120

gccgacggcc gcgacccgca guccaagacc agccucgccg uggcccggcg cgaggcccgg      180

gcgauguccc gccggcgcga ccgguaccug cgccggcgca aggccacgcu gcggaccaug      240

aucgaguacg ggcucaugcc cggcgacaag gccggccagg aggccgugcu gcgcgagacc      300

ggggaccggg agggcgggga cgagagcuuc aaccccuacg cgcugcgcgc ccgggcccuc      360

uccgagaagc ugcccccggc cuacaucggc cgcguccugu uccaccucgg gcagcggcgc      420

ggcuucaaga gcaaccggaa gaccgaccgc aaggacaacg acaagggcaa gaucgcccug      480

gggaucgacg agcugacggc ggccaugcac cgcuccggca gccccacccu cggggccugg      540

cuggccaugc ggcgcgccga gggccacucc gugcggcugc gcgcgggcag cgagguguuc      600

gacgccgagg gguacgccuu cuaccccgag cggucccucc uggaggacga guuccgccag      660

aucugggacg cccaggccgu ccaccacccc gacauccuga ccccggagcg gcgcgcgcac      720

cucuuccggg ugauguucua ccagcgcccc cugaagaagc cccugguggg ccggugcagc      780

uucaaccccg ccgaguuccg ccucucccgg gcccacccgc uguuccagga guuccgccug      840

uacaaggaga ucaacgaccu cgaggucgug cugcccgacc agagccaccg gaagcugacc      900

cucgacgagc gcaacgcccu gguggccaag cugaagacgu cccggaaggc gagcuucucc      960

gcccuccgca agacccugaa gcugaccccc gaccucgucu ucaacaagga gagcgaggcc     1020

cggaaggagc ugcuggggga cgagaucuac agcgugcucg ccgacgcgaa gauguucggc     1080

gcccgcuggg gcggguucuc ccgcgcccgg cagugggaga ucaucacccg ccugaaggag     1140

gaggaggacc ccgccagccu guccagcugg cucaaguccg aguucggccu ggacgacgag     1200

cgggugacgg ccaucgcgaa cgccagccug ccggacgggu acggccgccu cggggagacc     1260

gcccuggccu ccaugcugga ggagcucaag accgccguca uccccgagag cgaggcggcc     1320

cggcugugcg gcuacgacca cuccaaccug gccaaggagc aggacgaggg gcuggacacc     1380

cuccccgagu accaggagau ccuggagcgc cacaucccgc ccggcacggg cgaccccgac     1440

gacaucuacg accugcggaa ggggcgcauc accaacccca ccgugcacau cggccucaac     1500

cagcugcggc gcguggucaa cgcccugauc caccgguucg ggcgccccca gcggaucgug     1560

guggagcucg cccgcgaccu gcagcugagc gagaagcaga aggacgacgu caaccggcgc     1620

aucgcgaaga acacccggga ggccgagggc cgcucccaga agcucaucga gauggggcag     1680

cuggacacgg gcuacaaccg gcugcuccug aagcuguggg aggagcucaa ccuggacaag     1740

ccggaggacc gcgugugcau cuacagcggc aagcccaucg ggaucgccau gcuguucucc     1800

ggcgaggugg acgucgacca cauccucccc uggagcaaga cccuggacga cucccaggcc     1860

aacaagcugc ucugccucaa gagcgcgaac cggcagaagc gcaaccgcac ccccuccgac     1920

gugccggagu ggcgggaccg cuacgacgag guccuggccc gggccgcccg ccucccccgg     1980

aacaagcgcu ggcgguucgc cgcggacgcc augcagcagu ucgaggccga gggcggguuc     2040

cuggcccgcc agcugaccga cacgcaguac cucagccgga uggcccugga guaccugucc     2100

gcgcucuacc ccagcgagga ggccgacaag agcggcgagc ugcgccagcg gaagcgcgug     2160

cacguggucc cggggcggcu gaccgagcuc cugcgccgga acuggggccu gaacacccuc     2220

cugcccgacc acaaccuggg ggagauggcc caggagaaga accgccggga ccaccgccac     2280

cacgccaucg acgccgcggu gaucggcacc acgucccgga gccuccugca gcgcaugucc     2340

ggggccgccg cccgccugga cgacguggcg uucgacgacc ucguccggac cguggugaag     2400

gacaacccgc ccuggcccgg cuuccgcgag gagcugcugg ccgccaucgg ccgggucacc     2460

gugagccaca agcccgacca cgggaccgug ucccgcgccg ccuacgcgca gggcaagggg     2520

cagacggccg gcaagcucca caacgacacc gccuacgggc ugaccggccu ggucgacgcc     2580

aagggcagcc cccucguggu gcggcgcaag cccuucaugg cccuggaggu caaggacauc     2640

gcguccaucc gggacaccga gcugcagagc gcccucuacg gggccaucgg cucccugacg     2700

gagaagaagg cccugcagga ggcccucauc cgcuuccggg acacccaccc gcaguucaag     2760

gggauccgcc gggugcgcgu gcuggagacc cugagcguca uccccaucgu ggaccgggac     2820

ggcaaggcgu acaaggggua caagggcgac gccaacuacc gcuacgaggu gugggagacc     2880

cucgacggca aguggcacac cgaggucgug uccauguucg acgcccacca gcccgggugg     2940

cagagccccu uccacaagca gaacccggcc gcccggcgcg ugcugaagcu gcagcagaac     3000

gacauggucg cguacgagca ccccgccgac ggcuacacga ucgcccgggu ggugaaguuc     3060

uccaacgaca agcgcaucua cuucgccagc caccgggagu ccgggagccu caaggcgcgc     3120

gacgccgaca agggcgaccc cuucuccuac uucgccaagg ccaacaacgg gcugcgggac     3180

aucaagugcc gccagguccg caucgacgcc gcgggccgga ucuucgaccc cggcccgcag     3240

gaccgcgaga cccggcaggc caacaagggg guguga                               3276


<210>  1582
<211>  3279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1582
auggccuaca ccaugggcau cgacgugggg aucgccagcu gcggcugggc gaucgucgac       60

cuggagcgcc agcggaucau cgacaucggg gugcgcaccu ucgagaaggc cgagaacccc      120

aagaacggcg aggcccucgc cgugccgcgg cgcgaggccc gguccagccg ccggcgccug      180

cggcgcaaga agcaccggau cgagcgccug aaguacaugu ucguccggaa cgggcucgcg      240

guggacaucc agcaccugga gcagacgcug cgcucccaga acgagaucga cguguggcag      300

cuccgggucg acggccugga ccgcaugcug acccagaagg aguggcuccg ggugcugauc      360

caccuggccc agcgccgggg cuuccagagc aaccgcaaga ccgacggguc cagcgaggac      420

ggccaggugc ucgucaacgu gaccgagaac gaccggcuga uggaggagaa ggacuaccgc      480

acgguggccg agaugauggu caaggacgag aaguucuccg accacaagcg caacaagaac      540

gggaacuacc acggcguggu gagccgggcc ucccugcucg ucgagaucca cacccuguuc      600

gagagccagc gccagcacca caacuuccug gcguccaagg acuucgagcu cgaguacauc      660

aacaucugga gcucccagcg gcccguggcc accaaggacc agaucgagaa gaugaucggg      720

accugcacgu uccugcccaa ggagaagcgc gcccccaagg ccagcuggca cuuccaguac      780

uucaugcugc uccagaccau caaccacauc cggaucacca acgcccaggg cacccgcccg      840

cugaacaagg aggagaucga gcaggucgug aacauggcgc ugacgaaguc caaggugagc      900

uaccacgaca uccggaaggu gcucgaccug uccgaggagu accaguucgu cggccuggac      960

uacgggaagg aggacgagaa gaagaaggug gagagcaagg agaccaucau caagcucgac     1020

gacuaccaca agcugaacaa gaucuucaac gagguggagc uggccaaggg cgagaccugg     1080

gaggccgucg acuacgacac cguggccuac gcccucacgu ucuucaagga cgacgaggac     1140

auccgcgacu accugcagaa caaguacaag gacuccaaga accggcuggu caagaacgug     1200

gcgaacaagg aguacaccaa cgagcucauc gagaagguga gcacccugag cuuccgcaag     1260

gucgggcacc ugucccucaa ggcccugcgg aagaucaucc ccuuccugga gcagggcaug     1320

accuacgaca aggccugcca ggccgccggc uucaacuucc aggggaucag caagaagaag     1380

cgcuccgugg ugcuccccgu caucgaccag aucagcaacc ccguggugaa ccgggcgcug     1440

acgcagaccc gcaaggucau caacgcccug aucaagaagu acggcucccc ggagaccauc     1500

cacaucgaga ccgcccggga gcucagcaag cgcuucgacg agcggaagaa caucacgaag     1560

gacuacaagg agaaccgcga caagaacgag cgggccaaga agcaccuguc cgagcugggg     1620

aucaucaacc ccaccggccu cgacaucgug aaguacaagc uguggugcga gcagcagggg     1680

cgcugcaugu acagcaacca gcccaucucc uucgagcgcc ugcgggagag cggcuacacc     1740

gagguggacc acaucauccc cuacucccgc agcaugaacg acuccuacaa caaccggguc     1800

cucgugauga cccgcgagaa ccgggagaag uccaaccaga cgccguacga guacaugggc     1860

aacgacaccc agcgcuggga cgaguucgag cagcggguga ccaccaaccc ccagaucaag     1920

aaggagaagc gccagaaccu gcugcugaag ggguucacga accggcgcga gcucgagaug     1980

cuggagcgga accugaacga cacccgccac aucaccaagu accucagcca cuucaucagc     2040

accaaccugg aguucucccc cagcgacaag aagaagaagg ucgugaacac guccggccgg     2100

aucaccagcc accuccgcag ccgguggggg cuggagaaga accgcgagca gaacgaccug     2160

caccacgcca uggacgcggu ggucaucgcc gugaccuccg acagcuucau ccagcagguc     2220

accaacuacu acaagcggaa ggagcgccgg gagcugaacg gcgacgacaa guucccccuc     2280

ccguggaagu ucuuccgcga ggaggugauc gcccggcugu cccccaaccc caaggagcag     2340

aucgaggccc ugcccaacca cuucuacagc gaggacgagc ucgcggaccu gcagccgauc     2400

uucguguccc gcaugcccaa gcgcagcauc accggggagg cccaccaggc ccaguuccgg     2460

cgcguggucg gcaagacgaa ggagggcaag aacaucaccg ccaagaagac cgcccuggug     2520

gacaucuccu acgacaagaa cggggacuuc aacauguacg gccgggagac cgaccccgcg     2580

acguacgagg ccaucaagga gcgcuaccuc gaguucgggg gcaacgccaa gaaggccuuc     2640

agcaccgacc ugcacaagcc caagaaggac gggaccaagg gcccgcugau caaguccgug     2700

cggaucaugg agaacaagac ccucguccac cccgugaaca agggcaaggg gguggucuac     2760

aacagcucca ucgugcgcac ggacguguuc cagcggaagg agaaguacua ccugcugccc     2820

gucuacguga ccgacgugac caagggcaag cuccccaaca aggucaucgc cgcgaagaag     2880

ggguacgacg acuggaucga gguggacgac agcuucaccu uccuguacuc ccuguacccg     2940

aacgaccuca ucuucauccg ccagaacccc aagaagaaga ucagccugaa gaagcggauc     3000

gagucccaca gcaucuccga cagcaaggag gugcaggaga uccugacgua cuacaagggc     3060

gucgacagcu ccaccgccgc caucgaguuc aucacccacg acggcagcua cuacgccaag     3120

ggggugggcg ugcagaaccu cgacugcuuc gagaaguacc aggucgacau ccuggggaac     3180

uacuccaagg ugaagggcga gaagcgccug gagcucgaga ccagcgacaa caaccacaag     3240

gggaaggacg ugaacuccau caagagcacg ucccgguga                            3279


<210>  1583
<211>  3279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1583
augaaguaca gcaucggccu ggacaucggg aucgccuccg ugggcuggag cgucaucaac       60

aaggacaagg agcgcaucga ggacaugggg gugcggaucu uccagaaggc cgagaacccc      120

aaggacggcu ccagccucgc guccagccgc cgggagaagc gcgggucccg gcgccggaac      180

cgccggaaga agcaccgccu ggaccggauc aagaacaucc ugugcgagag cggccucgug      240

aagaagaacg agaucgagaa gaucuacaag aacgccuacc ugaagucccc gugggagcug      300

cgcgccaaga gccucgaggc caagaucucc aacaaggaga ucgcccagau ccugcugcac      360

aucgcgaagc ggcgcggcuu caagagcuuc cggaagaccg accgcaacgc cgacgacacc      420

gggaagcucc uguccggcau ccaggagaac aagaagauca uggaggagaa gggguaccug      480

acgaucggcg acauggucgc caaggacccc aaguucaaca cccacgugcg gaacaaggcc      540

gggagcuacc ucuucuccuu cagccgcaag cugcuggagg acgaggugcg gaagauccag      600

gcgaagcaga aggagcucgg caacacccac uucaccgacg acguccugga gaaguacauc      660

gagguguuca acucccagcg caacuucgac gagggcccca gcaagcccuc cccguacuac      720

agcgagaucg ggcagaucgc caagaugauc ggcaacugca cguucgagag cuccgagaag      780

cggaccgcca agaacaccug gagcggggag cgcuucgugu uccugcagaa gcucaacaac      840

uuccgcaucg ucggccuguc cggcaagcgg ccccugaccg aggaggagcg cgacaucgug      900

gagaaggagg uguaccucaa gaaggagguc cgguacgaga agcugcgcaa gauccuguac      960

cucaaggagg aggagcgguu cggggaccug aacuacagca aggacgagaa gcaggacaag     1020

aagacggaga agaccaaguu caucucccug aucggcaacu acaccaucaa gaagcucaac     1080

cugagcgaga agcugaaguc cgagaucgag gaggacaaga gcaagcucga caagaucauc     1140

gagauccuga ccuucaacaa guccgacaag acgaucgaga gcaaccugaa gaagcucgag     1200

cugucccgcg aggacaucga gauccugcuc agcgaggagu ucuccgggac ccugaaccug     1260

agccucaagg ccaucaagaa gauccugccc uaccuggaga agggccucuc cuacaacgag     1320

gccugcgaga aggcggacua cgacuacaag aacaacggga ucaaguucaa gcggggcgag     1380

cugcugcccg ugguggacaa ggaccucauc gccaacccgg ucgugcugcg cgccaucagc     1440

cagacccgga agguggucaa cgccaucauc cgcaaguacg gcacgcccca caccauccac     1500

guggaggugg cccgggaccu ggcgaagucc uacgacgacc gccagaccau caucaaggag     1560

aacaagaagc gggagcucga gaacgagaag accaagaagu ucaucagcga ggaguucggg     1620

aucaagaacg ucaagggcaa gcugcugcug aaguaccgcc ucuaccagga gcaggagggg     1680

cggugcgccu acucccgcaa ggagcugagc cugagcgagg ugauccucga cgaguccaug     1740

accgacaucg accacaucau ccccuacagc cgguccaugg acgacagcua cuccaacaag     1800

gugcuggucc ugagcggcga gaaccgcaag aaguccaacc uccugcccaa ggaguacuuc     1860

gaccggcagg ggcgcgacug ggacacguuc gugcugaacg ucaaggccau gaagauccac     1920

ccgcgcaaga aguccaaccu ccugaaggag aaguucaccc gggaggacaa caaggacugg     1980

aagucccgcg cccugaacga cacccgguac aucagccgcu ucguggccaa cuaccucgag     2040

aacgcgcugg aguaccggga cgacagcccc aagaagcgcg uguucaugau ccccggccag     2100

cugaccgccc agcuccgggc ccgcuggcgg cugaacaagg uccgcgagaa cggcgaccug     2160

caccacgccc ucgacgccgc ggugguggcc gucacggacc agaaggccau caacaacauc     2220

agcaacaucu cccgguacaa ggagcugaag aacugcaagg acgugauccc cagcaucgag     2280

uaccacgccg acgaggagac cggggaggug uacuucgagg aggucaagga cacccgcuuc     2340

ccgaugcccu gguccggcuu cgaccuggag cuccagaagc ggcuggagag cgagaacccc     2400

cgcgaggagu ucuacaaccu gcucuccgac aagcgguacc uggggugguu caacuacgag     2460

gagggcuuca ucgagaagcu gcgccccgug uucgugagcc ggaugccgaa ccgcgggguc     2520

aagggccagg cgcaccagga gaccauccgg agcuccaaga agaucagcaa ccagaucgcc     2580

guguccaaga agccccucaa cagcaucaag cugaaggacc uggagaagau gcagggccgc     2640

gacacggacc ggaagcucua cgaggcccug aagaaccgcc uggaggagua cgacgacaag     2700

cccgagaagg ccuucgccga gcccuucuac aagccgacca acuccgggaa gcgcggcccc     2760

cucgugcggg ggaucaaggu cgaggagaag cagaacgugg gcguguacgu caacggcggg     2820

caggcgagca acggcuccau ggugcgcauc gacguguucc ggaagaacgg gaaguucuac     2880

accgucccca ucuacgugca ccagacccug cugaaggagc uccccaaccg cgccaucaac     2940

ggcaagccgu acaaggacug ggaccugauc gacgggagcu ucgaguuccu guacuccuuc     3000

uaccccaacg accucaucga gaucgaguuc ggcaagagca aguccaucaa gaacgacaac     3060

aagcugacga agaccgagau ccccgaggug aaccugagcg agguccucgg cuacuaccgg     3120

gggauggaca ccuccaccgg cgccgccacg aucgacaccc aggacgggaa gauccagaug     3180

cgcaucggca ucaagaccgu gaagaacauc aagaaguacc agguggacgu ccuggggaac     3240

guguacaagg ugaagcggga gaagcgccag accuucuga                            3279


<210>  1584
<211>  3285
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1584
augaagucca cccuggccau cgccuccauc agcucccucg gcugggugau cgagcugggg       60

gagaaccgca ucaugcggcu gggccucgac aucgggagca ccuccaucgg cugguggauc      120

uacgagacga ccgacgggca gaucaccagc gucaucgacg gcggcgugcg caucuucucc      180

gacgggcggg accccaagag caagggcucc cuggcggugg accgccgggu cgcccgcgcc      240

cagcggcgcc ggcgcgaccg guaccugcgc cggaaggccg cccucaugaa gcgcauggcg      300

gagguggggc ugaugcccag cgaccccaag caggccaagc agcuggaggc ccucgacccc      360

uacgagcugc gggcccgcgg ccuggacgag acccucgagc ugacggagcu ggggcgggcc      420

cucuuccacc ugaaccagcg ccggggcuuc aaguccaacc gcaagaccga cgccggcgac      480

aacgagagcg ggaagaucau cgacgccacc gcccggcugg acugggccau gcgcgaccac      540

cgcgcgcgga ccuacggcga guuccuccac augcgccggc agaaggccga cacgccccgc      600

accaccccgg ccgugcggac ccgccugacg aucgccgccc gggacggggc ggagaaggag      660

gaguccggcu acgacuucua ccccgaccgc cagcaccucc gcgacgaguu cgacgcccug      720

ugggaggccc aggccgagua ccacggcguc cugaccgagg accugcgcga cgagaucggg      780

accaucaucu uccaccagcg gccccucaag acccccaagg ugggcaagug ccuguucacg      840

aacgaggagc gcguggccag cgcgcacccg cugaaccagc ggcgcauccu cgucgagacc      900

gugaacgccc ugcggguggc ccagcugggg gaggucgccc gcgcccucac ccuggaggag      960

cgggacacca uccugcacgc gcucgacaac aaggcccaca cgaagucccu gagcggcaug     1020

uccaucaagc ugaaggcccu cgggaaggug aucaagcugc gcagcggcca guacuucacc     1080

cuggagaccg ccaaccggga cgcgaucgcg ugcgaccccg ugcgggccag ccucagcaag     1140

cccgaccgca ucggcagcua cugguccacc cuggaccaca acgcccagug ggaggucgug     1200

aaccggaucc gcgccgugca gagcgacgcg gaccaccggg agcuggucgc cuggcucgug     1260

ucccagcacc gccuggccgc cgagcacgcc gagggggcgg ccuacgcgcc ccugccggac     1320

ggcuacagcc ggcucgggau cacggccacc aagcgcaucc uggccgcccu ggaggccaac     1380

gugauccccu acuccaaggc cgucgagagc ugcggcuggc accacuccga cgagcggacc     1440

ggggaggugc ucgaccgccu gcccuacuac ggcgagaucc uggaccggca cgugaucccc     1500

ggcaccuacg acgacagcga cgacgacauc acgcgcuucg ggcggaucac caacccgacc     1560

guccacaucg gccugaacca gcuccgccgg cuggugaacc gcaucaucga gguccacggg     1620

aagcccgacg agaucguggu ggagcuggcc cgggaccuca agcuguccca ggaccagaag     1680

gcggacgucg agcgcaccau caagaagaac acgcaggccg ccauccggcg cggcgagaag     1740

cuggaggaga ugggggugcc caacaccggc ggcaaccgga uggugcuccg ccugugggag     1800

gaccugaacg acgacgacgu caugaagcgg uucugccccu acaccgggca gcgcaucagc     1860

gccggcaugc uguucgacgg guccugcgac guggaccaca uccugccguu cagccgcacc     1920

cuggacgacg gcuucacgaa caagacccuc ugccugaagg aggccaaccg gaagaagcgc     1980

aacuacgcgc cccacgaggc cgccgagaag ggccugcucg accgggacgc cauggacucc     2040

guggccaagu accugcccga caacaaggcg uggcgcuucg cccccgacgc caucgagcgg     2100

uucgaggggg agcgcgacuu caccgcccgg gcccugaagg acacccagua ccucagccgc     2160

cuggcgcgga ccuaccugga cgcccucuac gacggcgggg acgacaaguc ccacgucugg     2220

guggugccgg gccgccugac ggagaugcug cggcgccacu gggggcucaa cggccuggag     2280

gcccuggccg acagcgacga gaagaccguc aagcggaaga accgcaccga ccaccggcac     2340

cacgcgaucg acgccgccgu gguggccgcc accgaccgcu cccucaucca gaagaucagc     2400

aagaucgcgg ccuccgacga gcaggccggc gccgaggaca cgcucaagag caccccgccg     2460

cccugggaca gcuuccggga cgacaucgcc gcgcagcugc agaacaucac cgucucccac     2520

cgcgccgacc acgggcggac cgacagccuc gccaagggcc gcggggagga cacgaccucc     2580

ggcgcccugc acgaggccac cgcgcugggg aucaucgacg agacccacgu gacggugcgc     2640

auccccaucg agagccucaa guccggccac cugaucgagg gcagccggaa cggguacauc     2700

cgcgaccccu accugcggga ccagcucacc cuggccacca agaacggcuc cgacaaggac     2760

uucaccacgg uccugcagaa guucuucgac cgcagcaagc gguacggggg cacccgccgg     2820

gugcgcauca ucaagacccu ccaggacacc gcccggaucu ccgugcccca caacgccccc     2880

aucaaggccu acaagggcgg cagcaaccac gcguucgaga ucuggcgccu gcccaacggc     2940

aagaucgagu cccaggucau cacgaccugg gagcaccaca accugaccga gcccaagcgg     3000

ccccacccgg ccgccaagcg caucauccgg cuccacaaga ccgacauggu ggccaucgag     3060

cgcgacggga agacggugau cugcuucguc cagaagaccg accccagcaa cggcgcguuc     3120

cuggugcccc acuucgaggc caacgccgac gggcggaagc gcaagaacga gcccguggag     3180

ugguaccaga uguccgccaa cagccugauc aaggccgacc uccggcgcgu cggcguggac     3240

gagauggggc gggugcgcga cccggggccc ccgcccaucc gguga                     3285


<210>  1585
<211>  3285
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1585
augaaguacc gcgugggcuu cgaccugggg accaacagcc ucggcugggc cgucgugcgg       60

cugaacgccu ccggggagcc cgaccgcauc gagguggcgg gcagccggau cuucuccgac      120

gggcgcgagc ccaagagcgg cgccucccug gccggcgccc ggcgcgacgc ccgggcgaug      180

cgccggcgcc gggaccgcuu caagcagcgg caggccgccc ucaucaagua ccugacccag      240

gccggguucu uccccgcgga cgagagcgag cgcaaggccc uguccgcccu cgaccccuac      300

gagcugcggg cccgcgcccu ggacgaggcg cucccccugg cccaccuggg ccgggcccuc      360

uuccaccuga accagcgccg gggguucaag agcaaccgca agacggaccg gggcaagaac      420

gacgacaccg ggaagauccg caucggcguc gcccgccugu acgagcagau gcgggacgcc      480

gacgcgcgca cccucggcga guuccugcac aagcggcgcg ccgccgccuc cgacccgaac      540

gccauccccc acgugcggac ccgcaucgug acgcuggcgg ccgaggacgg gaaggccagc      600

gacgucuacg acuucuaccc cgaccggcag acccucgagg aggaguucga ggccaucugg      660

gaggcccaga ccucccacca ccccgaggcg cugaccgagg agcgccgggc ccaguucuac      720

gagaucaucu uccaccagcg cccgcugaag cagcccaaga ucggcuucug cacgcuccug      780

ccgcccgaga agcggcugcc gaaggcccac ccccucuucc agcgccggcg ccugcuggag      840

gaggugaacg cccuccggau cgugcugccc gggaaggaga cccgcgcgcu ggagaaggcc      900

gagcgggacg cccuccugag caagcugaac cagcgcaaga aggccgccua cgagucccuc      960

cggaaggcgc ugaagcugga ccccgacgcc cgcuucaaca aggagagcga caaccgcgag     1020

gacaugaucg gcgacgagau cgccgccgag auggcccaca agucccgguu cggcaagguc     1080

uggcucggga ugagcgcgga ggcccaggcc caggugaucg agcgccugcg gggccuggag     1140

uccgacgccg acgccgacga cuucgaggcg uggcuccgcg acgaccacgg gcuggcccag     1200

gagcaggcca aggccgugac cgcggccaac cugccggagg gcuacgggcg guucggcgag     1260

accgccacgc gcaagcucau cgccgcccug gagcaggagg ucaucgugua cagcgaggcg     1320

gugcagcggg ccggccuggg gcaccacucc gaccuccgcg acggccaggu cuggaccgac     1380

aagaagggga accccgcccu gcccuacuac ggcgccgugc uggagcggca caucgugccc     1440

gggaccgccg acccgaacga gaccgacgac gcgcuccgca ucggccggcu gacgaacccc     1500

accguccaca ucggccugaa ccagcugcgc cgggugguga acgcccucau gcgccgguac     1560

gggcagcccc aggagaucgc caucgagcug gcccgcgagc ugaagcucag cgacgaccgg     1620

aagaaggaga ucaacacccg caaccgggag aaccgcaagg ccgcggagaa gcggagccag     1680

aagcugcgca acaucggcca ggccgacaag ggcgggaacc gcgcccugcu caagcugugg     1740

gaggagcuga accccgagaa cguccucgac cggcgcugcg uguacaccgg gcgggugauc     1800

uccaucgaca ugcuguucag cggcgccgcg gaggucgacc acauccugcc guucuccgug     1860

agccucgacg acuccaacgg caaccgcauc cugugccugc gggaggucaa ccgcaucaag     1920

cggcagcgca cgcccuggga ggcccgggac gaccuccgcg cccacuucgg gcccgacgcc     1980

ggcugggagg ccaucgcggc ccgggccgcc cgccugcccc gggagaagcg cuggcgguuc     2040

gagccggacg ccgcgaaccg cuucgacgcc cagggcgggu uccuggcccg gcaccucacc     2100

gacacccagc accugagccg ccuggcccgg uccuaccuca gcgcccugua ccccgagacc     2160

ggcgaggggu ccggccacgu gugggugagc cccgggcgcc ugacggagau gcuccgccgg     2220

gcgugguccc ugaaccacca ccucccggac cacaacaucg ccggcgccgc caaccagccc     2280

aagaaccgca aggaccaccg gcaccacgcc aucgacgcga ucgucguggc ccuggccgac     2340

cgcgccaugc ugaaccggau cgcgcgcgag agcgggaagg agggccacga ggccgccggc     2400

cggcuggugg ccuccauccc cgagcccugg gagggguuca gcgacgaggu cggccgcgcc     2460

gugcgggcga ucaccguguc ccaccgcccg gaccacggga ccgccagcaa ggccggccgg     2520

gccaaggaca aggacgccac cgcggggcgc cuccacaacg agacggccua cggccugacc     2580

ggcgagaagg acgcccgggg gcuggacauc gucgugaccc gcaagccccu cuccgccuuc     2640

aagaaguccg ccgaccugga cgcgauccgg gacgacgacc ugaagcagaa gcucaaggcc     2700

ugggccgccg gcaaggaggg cggggcguuc gagcaggcca ugcgcagcuu cggggacccc     2760

gagcggggcc cccgcagcca cccgggccug cggcgcaucc gggugaccga gccccugagc     2820

gucaucccca uccgcgaccg cgacgggcgg cccuacaagg ccuacaaggg cgacuccaac     2880

uaccgcuucg acaucuggga gcucaagacg gggaagugga aggacgaggu ggugagcaug     2940

uucgaggccc accggccgaa cuggaccucc cccauccgcg ccggcaaccc caccgcgcgg     3000

aagguccuga gcgugcgcca gaacgacgug aucgccaucg agcgggagaa cgggcccgag     3060

cugaugcgcg ucgugaaguu cuccgccggc agccucgccc uggccccgcc ccaggaggcg     3120

ggcucccuga aggcccggga cgccgacaag aacgaccccu ucaaguacgu guacgggagc     3180

cccuccagcc uccagcgcug gaaggcccgg cagguccgca ucgacgagcu gggccgggug     3240

uucgacccgg gguucgccgu gcgcucccgg cgcagcuccg aguga                     3285


<210>  1586
<211>  3291
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1586
augaagaaca ucuucagcuu cgacaucggc accaacucca ucgggugggc cgugcuggcc       60

cucgacggca acggggaccc cguccgcauc gcggacgccg gcgugcggau cuucagcgac      120

gggcgcgacc cgaaguccaa ggccagccug gccgaggccc ggcgcauggc gcgggccaug      180

ucccgccggc gcgaccggac ccugcgccgg aagcgcgcca cgcuccggac ccuggccgag      240

aucggccuga ugcccgacac cagcuccgcg cgccaggccc agcuccggga gaccagcgac      300

cccaaguccg gcagcgggca gcugacgcgc aaccccuacg cccugcgggc ccucgcccug      360

gaggagaagc ugccccucuu ccacaucggc cgcgcgcugu accaccuggg gcagcggcgc      420

ggcuuccagu ccaaccggaa gaccgaccgc aaggacaacg acaaggggaa gaucgcccuc      480

ggcaucgagg agcugcacgc cgccaugcgc caggacggcg cccagaccua cgggcagugg      540

cuggcgcggc gccacgcccu cggccaggcc gugcggcugc gcagcggggc ccuggaccuc      600

gagggcgagg gcuacgccuu cuaccccgag cggaagcugc uggaggacga guuccaccgc      660

aucugggagg cgcaggcccg gcacuacccc gacauccuca cccccgagcg ccgggaccac      720

cuguucacgg ucauguucuu ccagcgcccg cugaagaagc ccaagguggg gaagugcucc      780

uucaacaacc aggaggagcg gcucagcaag gcccaccccc uguuccagga guuccgccug      840

uacaaggagg ugaacgagcu cgagaucguc gagcccgacc ugacccacaa gaagcugacc      900

cucgaccagc ggaacgcccu ggugaagaag cuccaggugu cccgcgaggc cagcuucgcg      960

gcccuccgga agguccugaa gcugcgcccg gaccagcggc ucaacaagga guccgagagc     1020

cgcaccaagc ugaagggcaa cgagguguac gccgugcugu ccgacaagac gcgguucggg     1080

ccccgcuggg gcaccuucag ccaggccgag cagugggcga ucaucgagcg ccucaaggag     1140

gaggaggacc ccgggcggcu guucgccugg cugcgcagcg ccuucggcau cgagggcgag     1200

caggccgccg acaucgcgaa cguccaccuc cccgaggggc acggccggcu ggggaagacc     1260

gcccuggagg ccaugcucga ggagcugaag gccgagguga ucaccgaggc cgaggcggcc     1320

aagcgcugcg gcuacgacca cgcccggcuc gggggcggcu ccgacgaggg ggagccggag     1380

cugccgcccu accaggagau ccugcagcac cgcaucccgc ccggcacgaa caaccccgac     1440

gaccccuacg acaucgagaa ggggcgguuc accaacccca ccgugcacau cgcccugaac     1500

cagcugcgcc acgucaugaa cacccucauc cggcgcuacg gcaagccggc ccagaucgcg     1560

guggagcugg cccgggaccu gaagcucagc gagcgccaga aggaggaggu gaaccggacg     1620

aucgcccgca acacccggga cgccgagcgc cgggggaaga cccuggagga gaaccgcauc     1680

cccaacaccg gcuacaaccg gacgcugcuc cgccuguggg aggagcugaa ccccggcaag     1740

gucgaggacc ggugcugcau cuacaccggg aagcccaucu ccaucagcau gcuguucucc     1800

ggcgaggugg acaucgacca cauccugccg uggagccgca cccuggacga cucccacggg     1860

aacaagcucc ugugccugcg cgaggcgaac cgggugaagc gcaaccgggc ccccgccgac     1920

gucaccgagu ggcgcgacaa cuacgacgag auccucagcc gggccgcccg ccugccccag     1980

aacaagcggg accgcuucgc gccggacgcc auggagcggu ucgagaaggc cggcggcuuc     2040

cuggcccgcc agcucacgga cacccaguac cugucccgga uggcccugga guaccucgcg     2100

ugccuguucc ccgccgagga gauggacggg uucggcgagc ugcgccggca ccgcuacauc     2160

aacgugugcc ccgggcggcu caccgagaug cugcgccgga acuggggccu gaacagcauc     2220

cucuccgaca gccagcuggg ggagaccgcc caggagaaga accgcaagga ccaccggcac     2280

cacgccaucg acgccaucgu gaucggcguc acgggccgcu cccugcucaa ccgcaucagc     2340

uacgcggccg ggcggcugga ggcccagcag cuggagaccc ucgugaagga ggccgcggcc     2400

accaacaagc ccugggacgg cuuccgcgag gacgucgcca agguggucgg gggcaucauc     2460

gugucccaca agccggacca cgggaccgcc ggccggaagg ccaaggaccc cgcgcgccag     2520

cagacggccg gccggcugca caacgacacc gccuacgggc ugaccggcga gaccgacgcc     2580

cgcgggaaca ccaucguggu gcggcgcaag cccuuccuca gccugacguc caaggacauc     2640

cccagcaucc gggacgaggc ccugcgcgcg guccucgagc agcaccuccg gggcaucccg     2700

gacgggaagg ccuuccaggc cgcccuggac gccuuccgca agcagaaggg cuuccaccac     2760

uaccacggca uccggcacgu gcgccugacc gagccccugu ccgugaucgg gaucaaggac     2820

cgggacggca aggucuacaa gggguacaag ggcgacgcga accagcgcua cgacgugugg     2880

gagacccugg acgggaagug gcacgccgag guggucagca uguucgacgc ccaccagccc     2940

ggcuggcaga gccgguucca cgccgagaac cccaccgccc gccgggugcu ccgccugcag     3000

caggacgaca ugguggcgcu gaacgccccg gacggcgggc ggcuccuggu ccgcguggug     3060

aaguuccgcc agaacggcca gaucacguuc gccgggcaca acgaguccgg caaccugaag     3120

gcccgggacg cggccagcaa cgagcaggac cccuucaagu acaucucccg cauggccagc     3180

ucccucaagg acugcggcug ccggcagguc cgcaucgacg ccaccggcca gguguucgac     3240

cccgggcccc aggaccgggc cagccgccug cagaagcagu ccaagcggug a              3291


<210>  1587
<211>  3294
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1587
augaagaagc agccccagcg cuacauccug ggccucgacc uggggaucgc cagcgugggc       60

ugggcccuga uccucgucga cggggcggag aacccggugg gccugcugga cugcggggug      120

cggaccuucg agcgcgccga gguccccaag accggcgacu cccucgcccu ggcccggcgc      180

caggcccgga gcgugcgccg gcugauccgc cggcgcgcgc accggcuccu gcgccugcgg      240

cgccuccuga agcgggaggg cauccugucc gccgccgacu ucaacagcga cgggcucccc      300

aaccugccca ucgacgccug gcagcugcgc guggcgggcc ucgagcggaa gcugacgaac      360

caggaguggg ccgccguccu gcuccaccug gugaagcacc gcggguaccu gucccagcgc      420

aagagcgaga cccagaccga gaacaaggag cucggccggc ugcuggccgg gguggccgag      480

aacuccaagc uccugagcga guccagcgag caguaccgga ccccggcgga gcuggccguc      540

aagcuguucg ccgagcaguc cggccaccuc cgcaacaagg gcggggagua cacgcacacc      600

uucaaccgcc uggaccucca gaaggagcug caccuccucu ucgagcggca guacgcccug      660

ggcaaccccu ucgccagcgc ggagcugcag uccaaggugg acgagcuccu gaugacccag      720

cgcagcgccc ugcaggggga ggccgugcuc aagaugcugg gcaagugcac cuucgagccc      780

uccgaguaca aggccgccaa gaacacguac agcgcggagc gguucaucug gcugaccaag      840

cucaacaacc ugcgcauccu guacaacggc gaggagcggg cccucgacga gggggaccgc      900

aagaagcugc uggacgagcc cuacaagaag gccaagcuga ccuacaagca gguccggucc      960

auccucaacc ugccggagag cgccguguuc aagggccugc gcuacgggaa ggacuccgac     1020

ggccucaagg ccgagaacgg gacccugaug gagaugaagg cguaccacaa gauccggacg     1080

auccuggaca aggccggccu caagggcgag ugggagaacc gcauccggaa ccccgagauc     1140

cuggacaaga ucgggaccgc cuucagccug uacaagaccg acgaggacau ccaggccgcg     1200

cucgccgacc ugcgccugcc cgagaacgug cucgacgccc ugcuggccgg ccucaacuuc     1260

gacaaguuca uccagcugag ccugaaggcc cuccggaaca uccugccccu gauggagcgc     1320

gggauccggu acgacgaggc gugcaaggag gucuacggcg accaccgcgc ccucgccgag     1380

uccaagaacc aggugcugcu gccgcccauc cccgccgacg agauccggaa ccccgugguc     1440

cuccgcaccc ugacgcaggc ccggaaggug aucaacgcga ucauccgccg cuacgggagc     1500

ccgcagcggg ugcacaucga gaccgcccgc gagcugggca aguccaagca ggagcgggac     1560

aagaucgacc gcaagaacaa ggagaaccag aacgaccgga accgccucgc cagcgacauc     1620

cggaagcacu ucaacuucga gcccaccccc aaggacaucc ugaagcugcg ccucuacgag     1680

cagcagcacg gcaagugccu guacuccgac cugcgggagg agaaggggga gugccucuac     1740

accggcaacg ucaucgagcu gcaccgccug uucgaggacg gguacgugga gaucgaccac     1800

gcccuccccc ugagccggac gugggacaac ucccugaaca acaaggugcu cguccucaag     1860

agcuccaacc agaacaaggg caacaagacc ccguacgagu ggcuggacgg ggccaacaac     1920

agccgccggu ggcgcgaguu ccaggcgcgg gugcagggcu gccacuuccc cuacgucaag     1980

aagcagcgca uccagaccgc caagcuggac uccaaggccg agcagggcuu ccuggagcgg     2040

aaccugaacg acacccgcua caucagcaag uucaugugcc aguucaucac ggccaaccuc     2100

cggcuggcgg agaccuccaa cgcccugaug gaggaggugg ccggggagaa ccccuacugg     2160

aaggagcgca agcggcagcg cguguucgcc cccagcggcu ccaucaucgc ccuccugcgc     2220

gggcuguggg gccucaagaa gguccgggag gacaacgacc gccaccacgc gcuggacgcc     2280

aucguggugg ccugcagcac cgucuccaug cagcagaaga ucaccaaggc caugcagcgg     2340

cgcgagacgc uggagaccgu ggacagcgag accggcgagg ugaagacccg gaucccgcag     2400

cccugggacu ucuuccgcca ggaggucaug auccgggugu ucuccgacag cccccgcgag     2460

gagcucgccg agaagcugaa cagccggccc gaggcgcugc acgacuucgu guccccgcug     2520

uucgucagcc gcgcccccaa ccggaagaug acggggcagg gccaccugga gaccgugaag     2580

uccgccaagc gccugagcga gaacaucucc gugguccgga agccccucac cgcccugaag     2640

cugaaggaca ucgagaacau cgugggguac ccggaccgcg agcccgcccu cuaccaggcg     2700

cugcgggagc gccugcucgc ccacaaggac gaccccguga aggccuucgc cgacaagccc     2760

uucuacaagc cggcgaagga cggcgggcag ggcagccugg ucaaggccgu gcggaucauc     2820

gagaacacca agcacggccu gaagauccgc aacgggauca cggacaccaa cggcgacaug     2880

gugcggaccg acgucuacca gaagaccgac aagaagggga agcgccagua cuucaucgug     2940

cccguguacg ccuggcaggu cgagaagaac gagcucccca ccguggacug caagggcuac     3000

gagaucgacg acaacuacga guucugcuuc ucccuguaca aguacgaccu gaucgagauc     3060

cagaacccca acgacaucaa ccaggagcuc cgcgccuacu acaucaacug cgacagcucc     3120

gacgggcggu ucuggcuggc cuaccacgac aagagcgcga aggagcagca guuccgcauc     3180

uccacgcaga accagccgau caucaagaag uaccaggugg acacccuggg caaggagauc     3240

cggcucugca aggccgagaa gcgcaccgcc cugaacggca agaagaagaa guga           3294


<210>  1588
<211>  3294
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1588
augggcaagg gggacauccu guucagcuuc gacaucggca ccaacuccau cggguggugc       60

guguucgccc ucagcgacaa cggcgacccc ugccgcaucc uggacgccaa cgcgcggauc      120

uucgccgacg ggcgcgagcc gaaguccggc aacagccugg ccgagggccg gcgcgccgcc      180

cgggcgaugg cccgccggcg cgaccgguac aagcgccggc gcaaggccgu ccuccggacc      240

cugacggagu acgggcugau gcccgccgac cccaaggcgc gccaggcccu cguggccaag      300

accggcgacc gggggcccaa guccgaggag gcccuggccg acguguacag ccugcgcgcg      360

cgggcccuca uggagaagcu gccgcugcac cacaucggcc gcgcccucuu ccaccugaac      420

cagcggcgcg gguucaaguc caaccggaag accgaccgca agagcaacga gaagggcgcc      480

aucgagaccg gcgucaaccg ccugcucgag gagaugcggg acacgggggc ccccacccug      540

ggcgcguacc uggccaagcg ccgguccgac gggcgccccg ugcgggugcg cgccggcgcc      600

agcgagcagg aggccgagga cggcaagccc gcgaagaccg ccuacgacuu cuacccgguc      660

cggcgccucc uggaggagga guucgacgcc aucugggaga agcaguacuc cuaccacccc      720

gaggugcuga ccgaggagcg gcgcgggcac cucuuccggg ugauguucca ccagcgcccc      780

cugaagcccg ccaucgucgg ccggugcagc uucaacuccg ccgagacgcg ccuggcgaag      840

gcccaccccc ucuuccagga guuccggcug uacaaggagg ugaacgagcu ggaggugguc      900

augcccgacc agagccaccg caagcucacc cugcccgagc gggaccgccu ggugcucaag      960

cugcgggggc agcgcaaggc caccuacgcc gcgcugcgca aggagcuccg gcugcgcgac     1020

cugcgguuca acaaggagac cgagaaccgc acggacauga ugggcgacga ggugaccgcc     1080

gcccucgccg acaaggccgu cuucgggaac cggugguccg gcuucagcac cgacgagaag     1140

uggcgcaucg uggagcggcu gcgcgaggag gaggaccccu ucgcgcugca cgaguggcuc     1200

guguccgagc acggccugag cgacgccgag gccgagcagg ccgccaaggu ccaccucccg     1260

gacggguacg gccggcucgg gcccaccgcg guguccacga ugcuggagga gcugaaggcc     1320

gacgugauca ccgaggccga cgccgcccgc cucgcgggcu acgaccacag ccuggcccgg     1380

gccgacgagg acgggcugga ccgccucccc aaguaccagg agguccugga gcggcgcauc     1440

ccgccgggca ccaacgaccc cgacgacccc uacgacaucg ccaagggccg gaucaccaac     1500

cccacggugc acaucgcgcu gaaccaguuc cgccacgugg ucaacgcccu ggcccggcgc     1560

uucgggaagc cggaccagau cgccaucgag cucgcccggg agcugaagcu gaacgaccgc     1620

cagaaggacg aggugaaccg ggucaucggc cgcaacaccc gcgcggccgc ccuccggagc     1680

cagaagcugc gcgacaucgg gcagaaggac aacggcuaca accgggcccu gcucaagcug     1740

ugggaggagc ugaaccccga caagcccgag gaccgcgugu gcguguacac cgggcggccc     1800

aucugcaucu ccaugcuguu cagcggcgag gucgacgugg accacauccu gccguggucc     1860

cgcaccaugg acgacagcca ggccaaccgg auccugugcg uggucgaggc gaaccgcgug     1920

aaggccaacc gggcccccgc cgagguggcc gaguggggcg ggcgcuacga cgagauccuc     1980

gagcgggcgg cccgccugcc ccggaacaag cgcuggcggu ucgcccgcga cgccauggag     2040

aaguacaagg agaacgaggg cuuccuggcg cggcagcuca cggacaccca guaccugucc     2100

cgccuggccc gggaguacgc cgaggcccuc uacccgaccg aggagcccga ccgccugggg     2160

gagcugcgcc ggcgcaacca cguccgggug auccccggcc gccucaccga gaugcugcgg     2220

cgcggcuggg ggcugaacgc ccuccugccc gaccacaacu ucggcgcggg gcaggccaag     2280

aaccggaagg accaccgcca ccacgccauc gacgccgccg uggucggcgu gacgacccgg     2340

gggcugcucc agcgcaucag caccgaggcg ggccugcggg agaagcuggc ccucgaggag     2400

accaucgccg acaucccgau gcccuggccc ggcuuccgcg aggagcugaa ggccgccauc     2460

gaccgggcgg uggucuccca caagcccgac cacgggacgc ugccgaagcc cggcgagcac     2520

gggcgcaccg ccggccagcu ccacaacgac accgccuacg ggaucaccgg cgagacggac     2580

gcccggggca accccaucgu ggugcgccgg aagcccuucc ugagccugac cgacaaggac     2640

aucccgucca uccgcgacac cgagcuccgg gggcgcgucu acagcgccac cuccggccug     2700

agcgggcggg agcuguccgc ggcccucgac uacuuccgca agaccgacaa gcaguuccgc     2760

ggcauccggc acgugcgcgu gacggagagc cuguccguca uccccauccg ggaccgcgac     2820

gggaacaucu acaagggcua caagggcgac gccaacuucc gguacgacgu gugggagcug     2880

cccgacggga aguggaaggc cgacgugguc accauguucg acgcgcaccg ccccgaccgg     2940

gccgccgagg agcgccggcc gcaccccgcc gcccgcaagg ugcucagccu gaagcagaac     3000

gacauggugg cgugcgagca ccccaaggac ggcuacacca ucgggcgggu cgugaaguuc     3060

aacaccgccg gccagaucua cuucgccgcc cacaacgagg gcgggucccu gaaggcccgc     3120

gacgcggacc gggccgaccc cuucaaguac uucagcaagu ccgccagcgg ccucaaggag     3180

aucgccgccc gccaggugcg gaucgacgcg acggggcagg uccuggaccc gggcccccag     3240

gaccgcgaga gccggcuggc ccgcaacaag cggcucgagc gcgagcgcgc cuga           3294


<210>  1589
<211>  3303
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1589
augaaguaca ccaucggccu ggacaucggg accagcuccg ugggcugggg cgucaucgac       60

acggagcgca accggaucca ggaccucggg gugcgccugu ucgagagcgc cgagaaccag      120

aaggacgggg agucccuggc caagccccgg cgcgacgcgc ggagcaugcg ccggcgccuc      180

gcccggcgcg gcucccggcu ggagcgcguc aagagcaucu ucgagaaggc caaguuccug      240

acccggggcc agauggggau cacccacgcc cgcccgaacg accccuaccg gcuccgcgcc      300

gagggccugg accggcugcu cuccaacgag gaccuguuca ucgcggugua ccaccuggcc      360

aagcgccggg gguacaagag caaccgcaag aacgugcucg agggcgacgg ggaggacgug      420

cgggagggca aggccguccu ggacggcgug gcccagaaca agaagcugau ggcguuccgc      480

ggguaccgca ccgugggcga gauguucuuc aaggacuccg ucuucacgga ggccaagcgg      540

aacaaggccg ggagcuacaa gcacugcgug cuccgcgaga ugcuggagga cgagcugggc      600

aagauccucc acgcccagcg gucccugggc aacacccugg ugaccgacag cuucaucucc      660

aagcuccugg agaagaagac cggggucuuc guguaccagc gccccuucgc cagcggcaac      720

cagauccuga agcucguggg gcccugcacg uucgagccgg aggagauccg ggcggccaag      780

gccaccuacu ccuuccagua cuucaacguc cugcagcgcc ugaacaaccu caccaucaag      840

aacaccgaca cgggcaccga gcggaagcug accagcgagg agcgcgccaa gaugaucgcc      900

uucaucuucu cccacaagga cgugcaguac gcgcagaucc ggcuggagcu caagcugagc      960

gacguggacc gcuucaacau ggucaacuac uucgugcagc ggaaggagcu ggagaccaag     1020

acggccgagg agcuccgccg ggagaucgag aagaagacca aguuccccuc ccugagcgac     1080

uacuacacca uccgcaagac cgugagcgag aucgacuccg ccuucuggau ccagaucgag     1140

aagcagcacg acgucaucga cgccaucggg gagaucuuca cgcuguacaa gaccgacgag     1200

gacaucaucg cccagcucaa cacccggauc cccagcaucc cccgcccggu gguguccgcg     1260

cugcugggcc ucagcuucac caaguucggc caccuguccc ugaaggcccu ccggaagauc     1320

aucccccacc uggagaacgg ggacacguac gacgucgccu gcgccaaggc cgaccccaag     1380

uacaccgacc gcgcgaagac ccgggcccgc aagcugcgcc cccucgacaa ggaggaccac     1440

agcaucacca acccgguggc ccggcgcgcc aucgcgcaga cgaucaaggu cgugaacgcc     1500

aucaucgaca aguacgacuc ccccagcgag gugcacaucg agcugggccg ggaccuggcc     1560

aagaacuuca aggagcgcau gaagauccag aaggagcaga agcagaacca ggaggggaac     1620

gagcgggcca ucgccaagau ccacgagcuc uacaagcugg agaacccccg cggccaggac     1680

aucaucaagu uccggcugug gaacgagcag aacgggcagu gcauguacuc cggcgagcgc     1740

aucgacgaga uccggcuguu cgacaacggc uacaccgaga ucgaccacau cauccccuuc     1800

agccgcuccu ucaacgacag cuacuccaac aagguccucg ugcugaagau gcggaaccag     1860

gagaagauga acaagacccc guacgaguac augggcgggg acgcggacac cuggcagcgc     1920

uucgagaacc uggugaacag caugcacaac aucgggcccc ggaagaagca gaaccuccug     1980

cugaagaagu acgucgccga cgaccucacg cugcgcaccc ugaacgacac ccgguucauc     2040

gcccgcuaca ugaagaacua caucgagaac acccuccugu ucacggaggg cccccagaag     2100

cagcgggugu acaccgugaa cgggcaggcc accgccuacc ugcgcaagcg guggguccuc     2160

cacaaggacc gcaccgagaa caaccggcac cacgcgcagg acgccguggu ggucgccgug     2220

gccacccccu cccuggugca gggcaucaug cgccacgcca agaugggcga gaucgucgac     2280

uaccugcaca gccagaaguu cgcgccgaac gugacggaca ucgagaccgg gaagcccuuc     2340

gaguccgccg acauccagag cgccaaggag cgcaucaucg ccaagcacga gcaccggcuc     2400

cgcauccccg agcccuggga gggcuucucc gacgagcugg acgcgcggau gggggacgac     2460

guggucgaga agcugagccu ccgcaagagc aacauccacg gcuacaagac caccuccgac     2520

uucagccaga uccacccgau cuucgugucc cggaugccca agcgcaagac gaccggccgg     2580

gcccaccagg agacccugcg cagcccccgg cgcuucaagg aguuccagga guccgugguc     2640

cggacgcccc ugagcuccau caaccucaag aagcuggagg agaugagcgg gaagaacucc     2700

gacaagcugc ucuacgacau ccugcgcaag cggcugcucg cccacggcga cgacccgaag     2760

aaggccuuca ccgagcccgu gcacaagccc cgcaaggacg ggacccaggg cccgcuggug     2820

cggagcauca agauggucuc cgaggggcag cgcagcggca uccuggugaa caacgagcag     2880

gcccucgugg accaggacuc caugauccgg aucgacaucu ucagcaagcc caacaagaag     2940

ggcaaggacg aguacuacau cauccccguc uacgcguacc acuucgccca gaaggagcug     3000

cccaacaagg ugaucaccgc caaggccgag gccgacuggc gcaccaucga cgacacguuc     3060

gcguucgagu ucucccugua cccgaacgac cucgugaagc ugaccaaggg ggacgacauc     3120

cgguucggcu acuacagcgg gugcaaccgc gcccuggccg ccauccacau ccucccccac     3180

gaccagaaca ugccccuggu caacaacggc aucaagaccc uggacaagau cgacaaguac     3240

gugguggaca uccucgggga guaccacaag gucggcaagg agacccgcca gcagauccag     3300

uga                                                                   3303


<210>  1590
<211>  3303
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1590
augggcgcca ccaucgugac cgacaucagc cgcuccgccu ucgcguucga caucgggacc       60

aagagcaucg gcuccgccgu ccugcggcuc gacgaccgcg gggagcccau cggcaucauc      120

aacgccgccg ugcggaucua cgccgacggg cgcgagccca agagcggcac gucccuggcg      180

gagggccggc gccuggcccg ggggaugagc cgccggcgcg accgguacaa gcgccggcgc      240

cggacccucc ugaacacccu gacggaguuc ggccucaugc cgcccaccga cgaggcccgc      300

aaggcccugc uggcggagac caacgaccgg cgcuucgggg ccguggccgg caacgucuac      360

gcccuccggg cccgcgcgcu ggacgagaag cugccccucc accaccuggg gcgggcccug      420

uuccaccuca accagcgccg cggcuucaag uccaaccgga agaccgacaa gcgcgacaac      480

gaccagggca agaucgccag cgggaucucc cggcugggcc agaagcugga cgaggccggg      540

gcccgcacgc ucggcgaguu ccugcaccgg cagcacagcg cgggcaagug ggugcgcgug      600

cggccguccc gcguccccag caaggacgac aaggaggagg acggguacgg cuucuacccc      660

gagcgguccu uccuggagcg cgaguucgcc gccaucugga aggcccaggc cgcguucuac      720

cccgagcucc ugaccgagga gcgcggggac caccuccacc gggugauguu cuuccagcgg      780

ccgcugaagc gccccaaggu gggcaagugc agcuucuacc ccgacgagga ccggcugccc      840

aaggcccacc cgcuguucca ggaguuccgc cucgucaagg agcugaacga gcuggaggug      900

gugcaccgcg gggaccggcg ggagaagcuc acccuggagc agcgcgacac ccuguucaac      960

cagcuccgca cgcagcgguc cagcgccuuc accgcccugc gcaagacccu gaaggccccc     1020

accggcacgc gguucaacaa ggaguccgac gcgcgcgaca agcucaaggg cgacgagguc     1080

gccgccgaga ugagccacaa ggaccgguuc gggcaggccu ggaucggcau gccccuggcg     1140

gagcgcuggg ccgugaucga gaugcugcuc gacaccgagg acccccugga ggugaaggcc     1200

uggcuggagg agcacuacgg guucgccgcc gaccggaucg acgcgaucgc cgccgccaag     1260

cucccgcccg gcuacgggcg ccugggcgag accgcccugc gggacaugcu cgacgagcug     1320

aagaacggca ccgucaagga cgacuccggg cgcgacgugg ugcugacgga gcggcaggcg     1380

auggacaaca ucuggaagga caaccccgcc cuccagcgcg ucguggccga cgcccgggag     1440

cugcccccgu accaggaggu gcugggccgc cggaucccgc ccgggaccgg caagcccgag     1500

gaccccuacg acguccagaa ggggcgccug accaacccca ccgugcacau cggccucaac     1560

cagcugaagc ggguggucaa cgcccuggug cgccggcacg gccgccccga ccagaucgcg     1620

cucgagcugg cccgggagcu gaagcucaac gaggagcaga aggccgagaa ggaccgcgag     1680

aucgcccgca acacgaaggc ggcccaggcc cggagcgaga agcugcgcca gcuggacauc     1740

gacgacaacg gguacaaccg gguccuccug aagcuguggg aggaccucaa ccugcgccag     1800

cccgagaacc gggagugcau cuacugcggc aaggccaucg ggaucggcac ccuguucagc     1860

aaccaggugg acaucgacca cauccucccg ugguccaaga cccuggacga cagccaggcc     1920

aaccgcaucg ugugccacgu cgagugcaac cggcagaagg gcaaccacgc gcccgccgag     1980

gugccccagu ggcacgaccg cuacgacgac auccuggccc gggccgagcg ccucccgcgg     2040

aacaaggccu ggcgcuucgg gcgggaggcg augaagaagu ucaaggacga cgaguccuuc     2100

cuggcccgcc agcugaccga cacgcaguac cucagccgga uggcccacga guacguggcc     2160

gcccuguacc ccgacgagga gcccgacgag uggggcgucu ucacccgccg gaaccacgug     2220

cgcgugaucc ccgggcggau gaccgagaug cugcgccgcc aguggggccu caacgcgauc     2280

aucuccgacc acgaccagaa gaaccggaac gaccaccgcc accacgccau cgacgccauc     2340

gucguggggg ugaccacgcg gcgccugcug cagcggaucg ccaccgcggc cggcggccgc     2400

gaggcccucg cccuggagga gaccaucggg aagaucgagc cgcccuggcc cggcuucuac     2460

gacgacguca aggaggccau cgaccggauc guggugagcc acaagcccga ccacgggucc     2520

cugccgaagc ccggcgagcc cgcgcgcacc gccgggcagc uccacaacga cacggccuac     2580

ggcuucaccg gcgaggagcg gaaggggguc cccaucaugg ugcaccgcgu gccguucgcc     2640

acccugaccg agaaggacau ccccagcauc cgggaccgcc agcuggccga ggcgcucuac     2700

ggcgccaucg ccgggaucuc cggcaaggcc gagcuggccg cggcccuggc ccgguucagc     2760

gccuccgggc ccuacaaggg cauccgccac guccgggugg uggagcccgu cagcgugauc     2820

gccaucaagg accgcgacgg caccccguac aagggguaca agggcgacgc gaacuaccgg     2880

uacgacgugu gggagcuccc caacgggaag uggacgcacg aggucguguc cauguucgac     2940

gcccaccgcc ccgacuggca gagcgagauc cggaacaaga accccgccgc ccgcaaggug     3000

cugucccugc accagaacga cauggucgcg uacgagcacc cgcaggacgg cuacaccauc     3060

gggcgggugg ugaaguucgg cagcggcggg cagauggccc ucgccggcaa ccgcgagggc     3120

ggggcccuga agucccgcga cgccgacccc gaggaccggu uccgcuacuu cuacaagagc     3180

ggcggggcga ugcgggacgc ccgccugcgg cagguccgcg uggacgagac cggccgggug     3240

uucgaccccg ggccccagga ccgcgagucc cggaacgccc gcaagcagag cgccgccggc     3300

uga                                                                   3303


<210>  1591
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1591
augaagaccc ugggccucga ccuggggacc aacagccugg gcugggccau cguggagacg       60

cgcgacgagc ccaccaagua cuccgagggg agcaucgucg ccuccggcgu gcggaucuuc      120

agccaguccg agauggcggg gcgcgacccg cagagcaagg ccucccucgc cguggcccgg      180

cgccaggccc ggggcagccg ccggcgccgg gaccgcuacc ugaagcggcg ccagcggcug      240

gucgaccucc ugguggaccu gggccucaug cccgcggaca aggccgcccg cgccgagcug      300

gugaagaacc acggggacgg caagaccggg gaccugucca gcuccgucua cgcgcuccgg      360

gcccgcgccc uggacgaggc ccugagcccc uacgagaucg gccgggccau cuuccaccuc      420

aaccagcgcc ggggguucaa guccaaccgc aaggcggaca gcaacgaccc cgagcagggc      480

gugaucuccg agggcaugcg ggcccuggac gagaagaugg ccgaggccgg ggcccgcacc      540

aucggcgagu ggcugcacau gcgccggcuc caggggcugc cggugcgcgc gcggaucgcc      600

cccgacggcg agggcuacgc cuucuacccc agccgcgggg cccuggagcg ggaguucgac      660

cgcaucaugg cccggcagac ccucuuccac acggaccugc ugaccgacac cgcgauccgc      720

gagcuccggg aggucguguu ccaccagcgc ccccugcggc cggugaagcc cggcaagugc      780

uccuacaacc cccuggagga gcgccucgcc aaggcccacc cccuguucca gaaguuccgg      840

cugcucaagg agcugaacga gcuggagauc gucggggagg accagaagca caucaagcuc      900

acgccggagc agcgcaacgc ccuggcccug cagcuccgga ccagccugac caagcagggc      960

aaggugcccu ucuccaagcu gcgcagcacc cucaagcugg ggcgggaggu gcgcuucaac     1020

aaggagacgg acaaccggag cgaccuggag ggcgacguca ucuacuuccg caugucccgc     1080

cccgagugcu ucggcaaccg gugggcggac cuccccgugg agaugcaggc cgccaucacc     1140

gagcgccugc ggaccgagcc ggacuacaag acccugauga acuggcucaa ggccgagacg     1200

gggcuggacg aggugcacgc gcgcgccguc gccgacaccc ccgugcccga cggcuucggg     1260

cggcugggcc agagcgcccu caccgcccug aucgacgcgc uggagaacga ggugggggag     1320

ggcggcuucg ucaucaccga ggccgaggcc gccaagcgcg uguacgggcg gacgaacucc     1380

gaggccgacc ccgaccgcaa gggcguggac gagcugccga aguaccagga gguccucgcg     1440

cggcacaucc cgcccgggac cggcgagccc gacgaccccu acgacgacuu cauggggcgc     1500

aucaccaacc ccaccgugca caucgcccug aaccagcugc ggcgcgucgu gaacgcccuc     1560

auccggacgu acggcaagcc cgaccgcauc gccaucgagg ugggccggga ccugaagaug     1620

aacgaccgcg agcgggacga ggucaaccgc cggaucgggc gcaacaccaa ggccgcgauc     1680

gagcggggcg ugaagcugac cgagaccuuc cgccagcucg acacggggua caaccgccug     1740

cggcuggagc ucugggagga ccugaaccag aagcccgaga accgcgugug caucuacugc     1800

ggcaaggcca ucgccggcca caugcuguuc aacggggaga ccgacaucga ccacauccuc     1860

ccguacagca agacccugga cgacuccaag gccaaccgga uccugugcug caccagcugc     1920

aaccgcgaga agaagaaccg gccgcccucc cgcguccccc aguggcggag ccgcuacggc     1980

gagauccucg cgcgggccac gacccugccc aagaacaagc aguggcgcuu cgccgaggac     2040

gccaugaccc gguucgggga ggaggagggc uuccuggccc gccagcucac cgacaugcag     2100

uacaucuccc ggcuggcgcu gacguaccuc gcccaccugu acgacuacga ggagguggac     2160

cuggacggga ucuacaagcg ccacgcccgg gugcgcgccc uccccggccg gaugaccgag     2220

augcugcgcc ggcagugggg ccugaacgag cuccugcacg accacaaccu gaucgggggc     2280

gacgucgcca agcccaagaa ccgccucgac caccgccacc acgcgaucga cgccaucgug     2340

aucgccugca ccagccgggc ccugauccag cgccuggcca ccgcguccgc cgccgccgag     2400

gagcgcgggg cggagcgggu ggucgagcgg aucgacccgc ccuggagcgg cuuccgcgag     2460

gacgugcggg ccgccguggg ggccaucguc gugucccaca agcccgacca cggcaccgug     2520

ggccgcgagg gguacgccaa gggccggggg cagacggcgg gcaagcucca caacgacacc     2580

gccuacgggc acaccggcga gaaggacgcc agcggcaacg acaucgucgu gcgccggauc     2640

gccgugaccg acauccgcuc ccccgccgac auccggaaga uccgcacgaa cgcgcacggg     2700

cacagcgagc ugcgggaccg ccuguacgag gccacccggg gccucgaggg gaaggccuuc     2760

gagcaggccg uccuggacuu cgugaagggc gacgccaagu uccgcgggau ccggcacgug     2820

cgcaucaccg aggucgcgaa cccggugugg aucacccacg gcgacggccg gcacaagaag     2880

ggguaccugc ccggcgggaa cgaccgcuuc gacguguggg agcucgccga cggccgcugg     2940

gacgccgagg ucgugacgac cuucgacgcc caccggcccg gcuucgugcc ccgcaugcgg     3000

cuggagaacc acaacgcccg caagaucaug ucccugaaga agggggacau gaucgcguac     3060

aacgacccgg ucagcggcca gcggaucauc gccaucgugc gcaaguucga ccagcggaac     3120

aagcagcucu accuggaccc ccacaacgag gccgggaacc uggaccgccg ggagaaggag     3180

aagaccuaca agccccuccg ccccaugccg aacccccuga agaaguaccg gccccgccag     3240

gugcggaucg acgagaucgg ccaggucuuc gaccccgggc cgugguggga gcgccaggac     3300

ggcuga                                                                3306


<210>  1592
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1592
auggagcaga ccagccugga ccucucccag gccaugaaga agguggccca gccccagaag       60

ccguaccgcc ugggcgucga cgugggggug gcgagcaucg gcgccgccgc cgucaccgac      120

acggagaucc uggggaucca cgugcggacc uucgagaagg gcgagaucaa cgggaagucc      180

ggccgcaagc cccucaacgc cgacuaccgg cgccagaagg gccagcggaa gcgccugcgg      240

cgcaagaaga accggcugcu cagccugcgc cggcuguucc gccggguggg gaugaucgag      300

uccgcggcca ucgaggccuu ccccaucagc uccaccagcc ccuggcagcu ccgcaccgag      360

ggccuggacc ggcgccugac gaacgaggag ugggcccagg uccucuacca caucgcgaag      420

caccgggggu uccaguccaa ccgccgggug gacauggacg aggccaccaa gcugagcgac      480

gccgccggcg acuccccgga gaccaccacg aacaccgaga gcgacuccga caccaagaag      540

augcuggccg ggguggcgga caacaagcgc cuccuggccg agaagggcua ccggaccauc      600

ggcgaggccg ucgcccgcga ccccaaguuc agccaccgca uccggaacaa ggaggagucc      660

uacagccaca cgaucgggcg cgacgagauc aacgccgagc ugaacugccu cuuccaggcg      720

cagaaccacu acggcaaccc ccacgccacc gaggagcugu ccgagcagau caucgaccug      780

aucaacuggc ggcgcaagac cgugaccggg gacgacauca agcggauggu gggccgcugc      840

acgcucgagc ccaaggagua ccgggccgcc auccacagcg ucuccgccga ggaguuccgc      900

uggcugagca cccugagccg gcuccgcauc gugacccagg gcgagucccg gccgcugacc      960

aagugcgagu gggacaaggu ggucgaccug cccuggcgcc aggaggcgau cacguacacc     1020

agcauccgga acgccaucgg gcuccgccac gacauccggu cccgcuucaa cauccgguac     1080

gagaccggcg ggaagaagac caagacggac accaagaacg agaccaccaa gggccagagc     1140

gccgacgccg ugacguccga ggugaagacc gccgagaagg agaccgcguu caaggccaag     1200

ggguaccaca uccugcgcaa ggccuacaag gccuucggcu acgacgagca gugggaccgg     1260

gaccgcuuca acaccgagcu gcucgacacg cuggcgaccg ccgucaccuu cuacaaggac     1320

gacagcgaga uccaggccca ccuggagacc aacgacacgc ccgaguacgc cauccagauc     1380

gcccuccgcc ugcccacccu gaccaaguuc uccaagcuca gcaacaaggc gauccugaag     1440

auccugccgc acaugcggga gcgccagucc uacagcaacg ccugccuccu ggccggcuac     1500

aagaaccacc acaagcagua cgacgacacg ccccagaacg ggaagauccc ccgguccgcc     1560

aucgagcaca ucaagaacac cgugguguac cgcagccuga accaggcccg gcgcgucauc     1620

aacgcgaugg ugaaggagua cggcgcgccc caggaggugc acaucgagcu cgcccgggac     1680

auggagaagu ccgccaagaa gaaggacgag aucgaccugu ggaugaagaa gcgcaagaag     1740

aagaacgacg agcugcggga gcgcuucggg aagaaccuca gccgggacga gcugcugaag     1800

uacaagcucu acgaggagca gaacggccag ugcgccuacu cccugcagcc gauccagcug     1860

gggcgccugc ucgagcccaa cuacgucgag guggaccacg cccugccccg gagccgcucc     1920

auggacaaca gcuacaacaa caagguccug gugcucacca agcagaaccg ggacaagggc     1980

gaccgcaccc ccuucgagua cuucggcgag gaccaggagu ccguggacug gaagcgguuc     2040

accaacuggg ucgagagcaa cccgcagaug ccccaggcca agcgccggaa gcugcugacc     2100

aagagccucc agaaggggau ccuggacaac uucuccgccc gcaaccugag cgacacgcgg     2160

uacaucgccc gcgagcucaa gacccugauc gagcacuaca uccugaacac ccccgagcgc     2220

gagaagacca aguacgagcg gugcguggug aucuccggca agcucacggc gcagaugcgc     2280

cagcuguggg ggaucagcaa ggcccgggac gucuccgacc gccaccacgg cagcgacgcc     2340

gccgugaucg ccugcaucac ccccuccaug gugcggcgca ucacccggua ccugcagcuc     2400

caggagcgcc gggcgaugcc gagcgccccc gggaagggcg acgagcaccc cgccgucgug     2460

gcccgcgcca ucccccacgc gccggugccc uggcccaccu ucgagcacga ccuggaggcc     2520

gcccuggaga agguccucgu gucccggccg cagagccgca agugguccgg ccccauccac     2580

gccgacacga ucuacgggga gcacaaggac caggagacca acaccguggc ccccacccag     2640

cggaucaagc ugacgcagcu gaccaagagc aacuacaaga acaucgucgg cuacgacgag     2700

gagcgcaaca aggacuucau cgaccagauc gaggagcggc ucgagugcca caacuucaac     2760

gggaagcagg cguucggcac cucccagagc cccaucuacc gcaucaagaa gaacggcgag     2820

cuggggccgg aggugaagaa ggugaccauc aagcugccca ucaagaacuc cgucuuccgg     2880

auccgccggg gcuacgugaa gaacgccaag aacagcacga ucuacaucaa caucuaccag     2940

aacaccaaga ccgggaaguu cgagaugcac cccguguacg ccaaggaccu caccuccagc     3000

gccaacgcga gcaaguccuu cgaguacagc cccgaccugc agcuggucga cacgauccgc     3060

cgcggcgacu acaucgagau cacccggcgc aacggggagg ugaucuccgg cuacuacgcc     3120

acccacaaca ucagcaccuc cgugcucgag cugcagccgc acgacacgac cgacagccgg     3180

aacaaccgca cccccgccau caagaccucc cugaacaucg agcgguucca caucgacauc     3240

cucggccgca agcggcgcgu cgugcggcgc gccaagaacg ggcuggccga cgaccgggac     3300

aaguga                                                                3306


<210>  1593
<211>  3309
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1593
augggcuacc gcaucgggcu ggacaucggc aucaccagcg ugggguacgc cauccucaag       60

accgacgaga agggcucccc cuacaagauc gagcaccuga cgagcgucau cuucccgauc      120

gccgagaacc ccaaggacgg gaagucccug gcgaugcccc ggcgcgagca gcggggcgcc      180

cgccggcgca accggcgcac caaguuccgg aagcaccgca ccaagaugcu guucgugcgg      240

agcgagcucc ugaccaucaa gcagaucgag gccaucuacu ccgagaagca gaagcucagc      300

uccaucuacg agcugcgcac gaaggcccug aacgagcggc ucaccaacga ggagcuguuc      360

cgcauccugu acuucuucag cggccaccgg ggguucaagu ccaaccgcaa gagcgagcuc      420

accgagaagg acaugggccc cgugcugacc gccaucaagg acaucgagga gaagcuggcg      480

gacgggacgu accggacccu cggcgaguac auggagcugg acgagaagua ccaggagcac      540

aagcgcaaca aggacgggau cgaccgguac cugggcaccg ccaagcgcuc ccucaucgag      600

gacgagauca agcagaucau caucgcccag cagcaguucg gcaacaccaa gcugacgagc      660

ggguuccagg ucgccuucau cggcgacgag gagcagaccg ggaucuucaa cgaccagcgg      720

gaguucgagg acggcccggg caagcagucc cccuacgcgg ggaaccagau cgagaagaug      780

aucggcgccu gcaccuucga ggagaacgag aagcgcgccc ccaaggccag cuacaccuuc      840

caguacuucg acuuccugug caagcucaac aaccugaagu accgcgagac gaucgggggc      900

gaguaccggu uccugucccu cgagcagaag aacgugcuga aggagaaggu gcugaccacc      960

cagaagauca ccuucagcgg ggucaagaag cuccuggacc ugcccgagca cgcccaguuc     1020

aaccucguga acuacggcac gaaguccacc gugaaggaga ccgagaccaa gagcgucuuc     1080

uacgcgauga ucuccuacca caagcugaag aaggccaucc cggcccccau cuucagcaac     1140

cugagcacgg ccgagcucga cgagaucggc cgcauccuga cccuguacuc cagcgacaag     1200

gggcggaaga acgaguucua cucccgccuc gccauccccg aggagguggu ggaggcgcug     1260

cugcccauca acuucagcaa guucggcaac cucucccuga aggccaugcg gaagaucauc     1320

ccguaccugg agauggggcu cgucuacagc gaggccgcca cccaggccgg cuacgacuuc     1380

cgccacaaga agaucgacca ggcguacauc cacgagaacg ugaccaaccc cguggucaag     1440

cgggccgccu cccagacgau caagaucgug aacaacauca uccgcaagua cggggagccc     1500

gacggcauca acaucgagcu ggcccgggag cugagcaaga acuucuccga gcgcaagaag     1560

accgugaagu accaggagga gaaccgggcc cgcaacgagc ggaucgcgga caagcuccgc     1620

gagugcgagc ugcccgucaa cggcgccaac aucacccggc ugaagcuguu cgaggagcag     1680

ggggaguucg acccguacac cggccucaag auccccuucg accgcgccuu cagcgaggag     1740

uacgaggugg accacaucau ccccuacucc aagacgcugg acgacagcua cgggaacaag     1800

auccuggugu ccgccaaggc gaaccaggag aagggcaacc ggauccccau ggaguaccuc     1860

caggggaagc cggagcgcau caagaagcug gagagcauca ccgccaccau caagaacccc     1920

aagaagcggg agaagcugcu caagcagcag cugaccaagg aggacgucga cggcuggaag     1980

acgcgcaacc ugaacgacac ccgcuacauc uccagccucc ugcacgacua cuucaaccag     2040

aacaucuccu ucgccaagcu ggagagcgag aagaagaagc gggugaucgc cgugaacggc     2100

gccaucaccu ccaagcuccg cgcgcggugg ggguucaaca agauccgcga gaacggcgac     2160

gagcaccacg cccuggacgc ccugaucguc gccugcguga ccgacaagua caucaaggag     2220

aucaccagcu ucagcaagcg gaaggagguc cgcuucaacg agaaccucug gaagcgggag     2280

aagacgcccc aggagaucga gcaggaggug gugcacuuca agaaggacua cgaccgcauc     2340

uucaaccagg ccuucccccu gccguggacc aacuuccggg aggaggucuc cugccgcaug     2400

agcgagaacc ccgcggagcu gaugaagaac cacaccuggg agaccuacac ggacgacgag     2460

aucgccgccc uccggcccau cuucaucgug cgcaugccca acaugaagac cuccgggccg     2520

gcccaccagg agaccauccg gagcggcaag cuggaggaga agggccugaa caucucccgc     2580

gugagcaucg agaagcucaa gcuggacaag gaggggacca ucaaguccgg caacgcccag     2640

uucuacaagg acagcgggaa cggcuggcag cugcuccacg acaagcugaa ggucgcgcug     2700

gagaagcacg acggggacgg cuccaaggcc uuccccgacg gcguguucaa guaccaguac     2760

cucgacaagg agcacacggu gcggaagguc cgccuggugg ccaagagcac ccugcagacc     2820

ucccucaacg agaagaaguc cguggccgac aacgggagca ugauccggau cgacgucuuc     2880

cgcaacgagc agggcaagua ccaguucgug cccaucuacg cgaaggaccg gguggccaag     2940

aagcugccga accaggccgu cgccgugggg aagaucgcca aggaguggcc ccugaucgag     3000

gagagcgacu ucaaguucuc ccucuacagc aacgaccuga uccgcgugaa gcacaagaag     3060

ggcgucaagc acguguccaa ggacgggaac aaggaggaga aguccaagac cgacuucuuc     3120

ggcuacuaca cgggcgcgga caucgccacc gccagcauca gcgggaucag ccccaacaac     3180

uucuucgagu uccgguccau gggccuggcc acccucauca gcaucgagaa guacaaggug     3240

gacuacuucg ggaacuacca ccaggucaac gagaaggugc gccagaaguu ccccaagaac     3300

cgcauguga                                                             3309


<210>  1594
<211>  3312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1594
augaaguacg acaucggcau cgacaucggg guggccagcg ucggcgaggc cgugaucgac       60

caggagggga acauccugga ggcgugcucc aaccucuucg acgaggccga cgccgccagc      120

aacguggacc gccggaacuu ccgcgagggc cggcgcaaca agcggcgcga gcggacccgc      180

gucaacgacu ucaagaagcu guggaccaag uucggguucg agauccccaa gaacgugaug      240

aacgacacga uccugcuccg gaacaagggc aucaagugcg agcuggaccu gaccgagcuc      300

uacuccgugc ugcuguacau gcucaagcac cgcggcauca gcuaccugga ggacgccauc      360

gacgaggcga agggguccaa cuacgccaag ggcaucgccc ugaaccagaa ggagcucaag      420

gagaagcugc cgugcgagau ccagcuggag cggcucaaga ucuacgggag cuaccgcggc      480

gacugcaucg ucaagaagga ggacgaggac gaguaccacu ccaacguguu caccaucagc      540

gccuacaaga aggagcugga gauccuguuc cggaaccaga agcuccccga ggaguucauc      600

cagggguaca ugaagaucuu cgagcgcaag cgggaguacu acaucggccc cggcaacgag      660

aagucccgca ccgacuacgg gguguacacg accaacaagg acgaggaggg ccacuacauc      720

accgacaaga acaucuucga gaagcugauc gggaagugca gcgucuacaa ggacgaguuc      780

cgggcggccg gcgccuccua caccgcccag gaguucaacg ugcugaacga ccucaacaac      840

cugacgguga acaaccgcaa gcugaccgag aacgagaaga agaagaucgu cgagaagauc      900

aagagcucca acgccgugaa caugcggagc aucaucaaga aggugcucgg cgaggacauc      960

gagcaguucg ggggcgcgcg caaggacaag gccgacaagg agaucuacca caccuucgag     1020

gucuaccgga agaugaagaa ggaguucgag aagaacggga ucgacaucgc cgacuucucc     1080

cgcgagaagc uggacgagau cggcaacauc cugacccuca acacggagac cgaggggauc     1140

aagggcgccc ugcacgaggc caagaacagc uucaccuacu ccgagaucga gacccugauc     1200

gacauccgca agaagaacag cucccucuuc agcaaguggc agagccuguc ccugaagauc     1260

augaacgagc ucauccccca gauguacgcg cagccgaagg agcagaugca gcugcugacg     1320

gagaugggcg uguucaaguu caagagcgug aaguucaaga acuccaagua cauccccguc     1380

gaggagauca ucaaggacau cuacaacccc gugguggucc ggagcgugcg caccaccauc     1440

aagaugauca acgagaccau ccggaaguac ggguaccccg acaacaucau caucgagaug     1500

ccgcgcgaca agaacuccac ggaggagaag gagcggauca agaaggagca gagcaagaac     1560

gagaaggagu acgagggcau ccucaagcgc aucaaggacg aguacgacau cgagaucaac     1620

cgggacgacu accgcaagca ggagaagcug gcccugaagc ucaagcugug gaacgagcag     1680

gaggggcggu gccccuacuc cggcaagaug aucagcaucu acgggcugcu cgaggacccc     1740

cagcuguucg agaucgacca caucaucccc aaguccauca gcuucgacga cucccgcaac     1800

aacaaggugc uggucuaccg gagcgagaac cagaagaagg gcaacaacac cccguacaug     1860

uaccucucca agggcgacaa gaacuggaac uacgaccagu acaagagcua cgugcugggg     1920

cugaagaaca agggcaacau cuccgacaag aaggucaaca acaugcuguu cauggaggac     1980

aucaacaaga ucgacgugcu caagggguuc aucacccgca accugaacga cacccgguac     2040

gccagccgcg ugguccugaa caugcuccag uccuucuuca acgagaagga cggcugcccc     2100

acgaagguga aggugauccg ggggagcuuc acccaccaga ugcgcgucaa ccugcggauc     2160

aacaaggacc gcgacgaguc cuacagccac cacgccgugg acgccaugcu gaucguguac     2220

agccagcggg gcuacgaggc guaccgcaag auccaggagg ccugcgucga cuucgagacc     2280

ggcgagauca ucgacgagga gcgcuggcag cagugcaucu gggggaagga cuacgaccag     2340

gugauguacg aggccaagug guccaagauc cgggagaaga ucaucgaggc cgagaagaac     2400

gugaaguacc accaccgcgu cgacaagaag gugaaccggg gccucugcaa ccagaccauc     2460

uacgggacgc gcaucaagga cggcaagauc uacaagauca gcuccuacaa caucuacgac     2520

aacaaggagu gcgccagccu gcugaagaug aucaagggcg ggaaggaguc caagauccuc     2580

auguacaacu acgacagcaa gaccuaccgg gacaugauca agaucaucga cgaguacucc     2640

gacgagaaga accccuucgu ggcguacaug aaggagaccg gcgacuacuu ccgcaaguac     2700

gccaagaagc acaacgggcc caagaucgag aaggucaagu acuacaagga ggaggccggc     2760

agcugcaucg acaucuccca caaguacggg uacgagaagg gcagcaagaa ggugauccug     2820

ggcucccuga acccguaccg gaccgacgug uucuucaacg agcgccucaa gaaguacuac     2880

cugaucggga ucaaguacaa ccacaucaag ugcgagggca acaacuacgu caucgacgag     2940

gacgccuaca acgggcugcu ccgggacgcg ggcgugauca gccacgagaa cacgaucaac     3000

gaccuggaga aggugggggu caaguaccug uucucccucu acaagaacga caucaucaag     3060

uacgagaaga acggcgagau guucguggag cgcuuccuga gccggaccau ggaguacgcc     3120

aacaacuaca ucgagaccaa gcccauccac aagcccuucu uccagaagaa ggacaagaag     3180

ggcaacauca agaacuccca gaaccuggug gggcucagca agaccaagua caucggcaag     3240

cuggucacgg acgcccuggg gaacaccuac augguggaga aggagaaguu cucccucauc     3300

guggacaucu ga                                                         3312


<210>  1595
<211>  3315
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1595
augaagcuga uccucgcccu ggacaucggc aucgccagcg uggggugggc gguccuggac       60

aagaaguccg agaccgugcu cgaggccggc agcaacaucu uccccgaggc cuccgccgcc      120

gggaaccagg ugcgccggga gaugcgccag gcgcggcgca ucaagcggcg ccagcggacc      180

cgccuggacg acuucaacaa gcuguggcgg aagaacaacc ucagcauccc gcaguacaag      240

uccacggaga ucgucaaccu gaaggugaag gcccugcgcg aggagaucag ccucgacgag      300

cuguaccuga uccucuacuc cuaccugaag caccggggca ucagcuaccu ggaggacgcc      360

caggacgacu cccucagcuc cagcaacgcc uacaccaacg ggcugcgcga gaacgcgaag      420

gcccuggaga agcacuaccc cugcgagauc cagcgggacc gccuccagac caucggcaag      480

uaccggggcc agacccagau cacggacgac aacaacgaga agaucgaccu guccaacgug      540

uucaccaucg gggccuaccg caaggagauc aagaaggucc uggagaccca gaagcucuac      600

cacaacgagc ugaccgacga gcugugcgac gacuacaucc ucaucuucaa ccggaagcgc      660

aaguacuacg agggccccgg gaacgagaag ucccggacgg acuacggccg cuucaccacc      720

cggcuggacc agaacgggaa cuacaucgac gaggagaaca ucuucgagaa gcugaucggc      780

aagugcagcg uguaccccga cgagcuccgc ggcgccgccg cgagcuacac cgcccaggag      840

uucaaccugc ugaacgaccu caacaaccug acgaucaacg ggcgcaagcu ggaggagucc      900

gagaagcacg ccaucgugca gcggaucaag accagcaaca ccgucaacau gcgcaagauc      960

aucgccgacu gcaugggcga gaccaucgag aacuucuccg gggcccggau cgacaagagc     1020

ggcaaggagc aguuccacaa guucgagacg uacaacaaga ugcgcaagag ccucuccgag     1080

aucggcaccg acaucgacga guucagccgg gacgagcugg acaagaucgg guacaucaug     1140

accaucaaca ccgacaagga ggcgcugaug gaggccuuca acgaguccgg ccucaaccug     1200

gacgacagcg ugaaggagug ccugaucucc uuccgcaagg ccaacagcuc cagcuucucc     1260

aaguggcaga gcuucucccu caagaucaug aacgagcuga ucccggagau guacauccag     1320

cccaaggagc agaugacgcu gcucaccgag augggggugg ccaagggcag cuccggggac     1380

uucgccggcc ugaacuacau ccccgucgac gugaucagcg acgagaucua caaccccgug     1440

guccggcgcu ccgugcggau cagcuucaag auccugaacg cggugcucaa gaaguacaag     1500

guccuggaca ccaucgugau cgagaugccg cgcgaccgga acuccgacga ggagcgcaag     1560

cggaucaccg acagccagaa gcugaacgag aaggagaugg aguucaucga gggcaagcuc     1620

gcccagaucu acaacaucaa gcuguccagc agcgacuucu ccagccagaa gcagcugucc     1680

cugaagcuca agcuguggaa cgagcaggac gggaucugcc uguacagcgg ccgcacgauc     1740

gaccccgagg acaucaucaa gaaccccgac cucuucgaga ucgaccacau caucccccgg     1800

uccaucagcu ucgacgacuc ccgcagcaac aaggugcugg ucuaccgguc cgagaaccag     1860

aagaagggga accagacccc guacuucuac aucacccaca gcgacggcgc cuggaccuac     1920

gagcaguuca aggccaacgu gaugaaccug uccaagaaga aggaguacgc caucagccgc     1980

aagaaggugc agaaccuccu guacuccgag gacaucacga agcgggacgu ccugaagggg     2040

uucaucaacc gcaacaucaa cgacaccggc uacgcgagcc gcgugguccu caacaccgug     2100

cagaacuucu ucaaggccaa ggaggccgac accaagguga aggucaucaa gggcuccuac     2160

acgcaccaga ugcggagcaa caugaagcug gacaagaacc gcgacgaguc cuacagccac     2220

cacgccgugg acgcgaugcu gaugggguuc agcgagaugg gcuacgaggc cuaccggcgc     2280

cuccaggggg aguucaucga cuucgagacc ggcgagaucc ugaagaacga cauguggaac     2340

gagaagaugu ccgacgacgu guacgucgac uaccuguacg ggcggaagug gagcgccauc     2400

cgcaacgaga ucgugaaggc cgagaaggac gugaaguucu ggcacuucau cguccggaag     2460

uccaaccagg gccucugcaa ccagaccauc cgcggcaccc gggaguacga cgggaagaag     2520

uacaagauca acaaguucga cauccgcacg aaggagggcg ugaagcuguu caagaagcug     2580

gccuucagcg agaaggaguc cgaccgggag aagcuccugg uguaccugaa cgaccgccag     2640

accuucaaca accucgucaa gaucuucgag gacuacaagg acgcgcccaa ccccuucgug     2700

caguacgaga aggagaccgg ggacgugguc cggaaguaca gcaagcgcca caacggcccc     2760

cggaucgaca agcugaagua caaggacggc gagguggggg ccugcaucga caucucccac     2820

aaguacggcc uggagaagga cagccgcaag gugauccucg agucccuggu cccguaccgg     2880

auggacgugu acuacaagga gagcgacaag auguacuacc uggugggggu caagcagucc     2940

gacaucaaga gccagaacgg cgccaacgug aucgacgagg accucuacac ccgcauccug     3000

aucaacgaga agaugaucga gcccgggcag ucccgggacg accuggagca gcucggcuac     3060

gaguuccgcu ucagcuucua caagaacgac aucaucgagu acgagaagga gggcaaggug     3120

uucgucgagc gguucguguc ccgcaccaug cccaaggugc gcaacuacau cgagacgaag     3180

cccaucaaca aggccaacuu cgagaagcag aacccggucg ggcugggcaa gaccaagagc     3240

aucaagaagu accggaccga cauccugggg aaccgcuacc ucugcgagaa ggagaaguuc     3300

accaaguucu gcuga                                                      3315


<210>  1596
<211>  3315
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1596
augaaccacu acgugcuggg ccucgacauc gggaucacca gcgucggcua cggggugauc       60

gaccuggaca acuaccaguu cguggacuac ggcguccgcc uguucaagga ggggaccgcc      120

gaggacaacg ugaaccggcg cacgaagcgg ggcucccgcc ggcucaagcg ccggcgccag      180

acccggcugg acgacaugaa ggagcugcuc cagaaggaga agaucaugag cgagaccuac      240

cacuccagcu acgaccccua cgacaugcgc aucaagggcc ugucccagaa gcugagcaac      300

gacgagcuga ccugcgccau ccuccacauc acgaagugcc gggggaccac ccuggaggcg      360

cucgacuccg ccgacgagga cagcgagggc accaagucca uccugagcaa gaacgaccag      420

gaccugaaga aguaccacua cgugugccuc guccagaagg agcgccugaa gcaguccggg      480

aagauccggg gcacggagaa caacuucaag accgacgacu acaucaagga gcuggacgag      540

auccucagcc accaggaccu guccgaggac cugugcgaga agauccgcac caucgugagc      600

cggcgccggc aguacuacga ggggccgggc uccgagaagu cccccacccc cuacggccgc      660

uggaucgccc aggggguggc ccccaucgac cucaucgaga agaugcgggg caagugcagc      720

gucuucccgg accagcugcg cgccgcgaag aacagcuaca cggccgagcu guucaaccuc      780

cugaacgacc ugaacaaccu cgagugcgag ggggagaagc ugaccaccuc ccagaagcag      840

gacgugaucg acuucgugaa cgagaagggc ggcaucaccc ccaagcagcu ggccaagcuc      900

cugaacgucg accugaacca gaucaagggg uuccggaucg acaagaacga caagccccuc      960

cugacggagu ucaagggcua caaggccuuc aagaagaucu ucgacaccaa caacugcgac     1020

cuguacaaga aggaccucag cauccuggac gggcuggcgg agaucgugac cggcaagaag     1080

gggaucgagg agcgcaagca ggccauccag caggccuacc ccgagaucga cgaggccauc     1140

guggagcagu gcgccgucau gaagggcaug acccaguacc acagccucuc ccugaaggcg     1200

augcaccuga ucaaccagga gaugcuccag agcccgcgca accagaugca gguccugcac     1260

gagucccacg uguucgacaa gaaccgggug agccacaagg gccagaagua caucgaggcc     1320

gacgacgagg ccauccuguc ccccguggug aagcgcgccc agcgggagac guucaagguc     1380

gugaacgccc uccgcgagaa guacggggag uucgagagca ucguggucga gaccacccgg     1440

gaccgcaacu cccaggagca gaagaagcgg cugaacgaca gccagaagua cuacgagcag     1500

gagaaccgca agguggacga gcugcucucc cacgagggcu acgaccccag caagaucaac     1560

gggaagacca agcugaaggu ccggcuguac cugcagcagg agggcaagac ggcguacacc     1620

aacgccccca ucgaccucaa ccugcugauc aaggacgcca ccgccuacga gguggaccac     1680

aucaucccgc ucuccaucag ccuggacgac uccaucaaca acaaggcccu gaucaaccgc     1740

accgagaacc agaacaagag caacaugacg cccaucuacg cgauccuccg ggggaagcug     1800

aacaucuccc uggacaccua caagaccgug gucaagaaca accgcaacua caccggcaag     1860

aagcggagcu accuccugua cgacaaggac aucacgaagu ucuccaacau gcaggaguuc     1920

aucgcccgca accuggugga caccagcuac gccugccggg uggucauguc cacccucagc     1980

gacuacuuca aggacaacga gaucagcacc cacgugcaca cggugcgcgg ccgggucacc     2040

aacauguucc gcaaccggau caacaugcag aaggagcgcg agaaggacua ccugcaccac     2100

gccguggacg cccugaucgu ggcguccauc aagaagcuca accugcugaa caccuaccuc     2160

cugaaguaca acuucaacga ccuguacaac gaggagaccg gggaggucuu cgccgugggc     2220

gacgaccagc agguccucga ccccaaguac auccgcuuca ucacccagcu gaagacgauc     2280

uacgacgaga gcaacaugua cuacaacggg cugaucgagc ggaacgugau gcaguucccg     2340

cccaucaaga ucucccacaa gaucgacacc aagcccaacc gccagaucag cgacgagacc     2400

aucuacucca cccggacggu gggcaacgag gaguacgugg ucaagaagua cagcgacauc     2460

uacgacccca aguucaccca gcucaccgac gacaucauca acgaccgccc ccuggaccgg     2520

uggcugaugu accgcaacga caaccagacc uucaagauca uccaguccau cauccucgac     2580

cacuuccaca ucuucaagga cgacgagaag cacuacagcu gcacgaagaa gaaggggcag     2640

auccaguacg cccugaaggg caagaacccg cuguaccucu acaagcagga gcacggcaag     2700

gugcggaagu acgccaagaa ggggaacggc cccgagauca ccaugaugaa guacuucgac     2760

gggaagcugg gcaacaacau cgacaucucc aaccacuacc acgugcacaa caagaagguc     2820

gugcugcucc agaucagccc cuaccgcacc gacuucuacg ugucccccga gggcaaguac     2880

aagaugcuga ccguccggua cgcgaacgug uucuacaagg agagcaagaa gaaguacgug     2940

aucgacgaga agugguacgc cuccgagaag aagcgcaaga acaucacgga cgacgacauc     3000

uucgucugca gccugcacca cgacgagcuc aucgggaucc agaagaagga cggcgccaag     3060

uacguguacg accuguccac cgaggacggg ggcaagccgc ggcuguacca cggggagaug     3120

gagauccuca aguucaccgc caccaacaac gacagcaaga acaucaucga ggugaagccc     3180

aucuacacgu acugcaagaa gcagcugaug aucuccaucg gccccaucac caagcuggcc     3240

aaguacgcga ccgacguccu cggcaacgug uacgagguga agaagaacau caucgaccac     3300

cugagccacc uguga                                                      3315


<210>  1597
<211>  3318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1597
augggcaucc gcaucgggcu ggacaucggc aucgccagcg uggggugggc cgucguggac       60

gacgacuaca acaucaucga guccggcgcg aacaucuuca acgaggccaa ggccgccagc      120

aacguggacc ggcgcacguu ccggcagacc aagcgccucc ugcggcgcca gaagacgcgg      180

cugcgcgacu ucgacauccu cuggaucaac gccgggcugg acauccccaa ggagaagguc      240

aacaagcagc ugcaguuccg ggugcagggc augaccaugg agcucuccga gaacgagcug      300

uacgcggccc ugaagaacau gcucaagcac cgcggcauca gcuaccugga ggacgcccug      360

gacgaggagc aguccggcaa gagcaccuac gagaaggggc uccaggugaa ccagaaggcc      420

cugaagugcg acaagaugcc gugcgagauc caguacgagc ggcugaugaa guacgggucc      480

uaccgcggcc acaucgaggu caaggacgag agcggggagg agaugauccu cuccaacauc      540

uucacccugg acgcguacug caaggaggcc aagaagaucc uggagugcca gaagcaguac      600

cacagcuuca ucacggaggc cuucgaggac gacuaccucg cccugcugcg gcgcaagcgg      660

aaguacuacg agggccccgg caacgagcag ucccgcaccg acuacgggaa guacaccacc      720

aagcucaacc ccgagacggg cgaguacgug accgaggaga acaucuucga gaagcugauc      780

gggaagugca gcguguaccc caaggagaug cgggccuccg cggccagcua caccgcccag      840

aucuacaaca ugcugaacga ccucaacaac cugaccauca acggccgcaa gcuggaguac      900

gaggagaaga aguccaucgu cgaggaggug cagaaggcca gcuccgugaa caugcucaag      960

aucaucaaga aggucaucaa cgaggacauc acgaccaucg agggcgcccg gaaggacaag     1020

aacgacaagg agaucuucca caagauggag accuacaaca agaugcgcca cuacuucgag     1080

aagaacgggc uggaguucaa gaagaucagc accgacgacc uggacgagau cggcaacauc     1140

cucacgcuga acaccgagaa ggaggcgauc cuggaggccu ucaacaaguc ccagcucgag     1200

cugagccagg aggagaagga ggccuucauc gagauccgga agaagaacgg gagccuguuc     1260

aacaaguggc acuccuucag ccucaagauc augaaggagc ugaucccgga gcuguaccuc     1320

caguccaaga accagaugca gcugcugacc gacaugggcg uguucaagaa gaagaccgag     1380

aucuuccaga acuacgacaa gauccccacg gacgugauca ucgaggacau cuacaacccc     1440

gucgugaagc gcagcauccg caucaccgug aagaucguca acgcccucau caagaaguac     1500

ggguaccccc gggagguggu gaucgagaug ccgcgcgacc ggaacuccga cgaggagaag     1560

aagcgcaucc aggacgagca gaagaagaac gacaccgagc ugaagaagau caucgagaag     1620

gucgagagcg aguacggcau caagaucacc gacacgcacu uccggaacca caagaaccug     1680

cgcaugaagc uccggcugug gcacgagcag gacggccgcu gccuguacuc cgggaaggug     1740

aucgacauca gcgagcuccu gaacgacugc uccaaguucg agaucgacca cgugaucccc     1800

aagagcaucu ccuucgacga cagccggucc aacaaggucc ugguguacag caccgagaac     1860

cagaagaaga ccaaccagac ccccuaccag uaccuggagg gcaacgggaa ggaguggucc     1920

uucgacgagu acaugaacca ggucaucgag cucaaggacc gcaagcugau cagcaaggcc     1980

aagaugaaca agcuccuguu ccgggaggac aucacgaaga ucgaggugcu gaagggcuuc     2040

aucgcgcgca acaucaacga cacccgguac gccucccgcg ugguccugaa cagcuuccag     2100

aaguacuucg aguccaagca gagcgacacc aaggugaagg ugauccgggg gagcuucacc     2160

caccagaugc gcaagaagcu ccagcugucc aagaaccggg acgagagcua cgcccaccac     2220

gccgucgacg ccaugcugau cugcuacucc cagaugggcu acgaggcgua ccgccagcuc     2280

caggaggacc ugaucgaccc cgacagcgag aagauccggg accugggccu cuggaaggag     2340

acgaucaccg aggccaagua cgacgaggug cuguacaccg ccaaguggcu ggagaaccgc     2400

gggaagaucc aggaggccga gaagaaggug aaguucuggc acaaggucga cuccaaggcg     2460

aaccgcgccc ucugcaacca gaccauccgg ggcacgcgcg ugaucgacgg gagcauccag     2520

aagaucaaca agaucaacau cuacuccgug gacggcuaca agaccuucaa gaacaugauc     2580

cagaagggga aggagaagga cuuccugaug uaccggaacg accgccggag cugggacgac     2640

cugaugaagg ucuaccgcga guacuccgac gccaagaacg ccuucgccgc guaccaggag     2700

gagaccggcg acuacgugcg gaaguacgcc aagaagcaca acggcagcaa gaucaccaac     2760

cucaaguacu gcgccaagga gcuggggucc ugcaucgaca ucagccacaa guacggccug     2820

gagaaggacu cccgcaaggu gauccucgag agccuguccc cguaccggag cgacgucuac     2880

uacaacgccg ggcugaagca guacuacauc gugggcauca agaacuccga ccucugcuuc     2940

cugaagggcg aguacuucau cgacagcaag cgcuaccugc agauccuccg ggaggagggg     3000

gugcugaccg agcagcacac gcugaacgac cucgccgcga ucggcaccga guuccaguuc     3060

agccuguacg agaacgacua ccuggaguac gagaagaacg gggagaucaa gaccgagcgc     3120

uuccucuccc ggaccaagcc caagcagaag aacuacaucg agaccaagag caucgacgcc     3180

cccaagauca agaacaagaa gcagaaccug gucggccugg ccaaguccaa gagcaucaag     3240

aagauccgcg uggacauccu cgggaaccgg uacuacgccu ccaaggagaa guucagccug     3300

augguggaca aguucuga                                                   3318


<210>  1598
<211>  3318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1598
auggacaugg ccuggcgccu gggccucgac cuggggacca acagccuggg cugggccgcg       60

cucucccugg acgccgccgg ggcccccgac gccauccugg cggccggcag ccggaucuuc      120

ggggacggcc gcgacccgca guccggcacc agccucgccg uggaccggcg cgccgcgcgg      180

gccgcccgcc ggcgccggga ccgcuucaag cagcggcagc gcgcccugcu gaagcaccuc      240

gaggccgacg ggcuguuccc cgcggacccc gagguccagc aggcccuggc cgcccucgac      300

cccuacgccc ugcgggcgcg cgcccuggac gaggcccucu cccugcacga gaucggccgg      360

gcccuguucc accucaacca gcgccggggg uuccagagca accgcaaggc cgaccggggc      420

aaggacgagg acgcggggaa gaucgccauc ggcguggacc gccugaagga cgccaucgcc      480

gccgcgggcg cccggacguu cggggaguuc cugcgccagc gccgggccgg cgccaccggg      540

gagaaccaga ucccguccgu gcgcacccgg cuccgcgcgg agaccggcga gggcgccaag      600

gggagcggcu acgacuucua ccccucccgg gcccugcuga aggacgaguu cgacgccauc      660

uggcacgccc aggcggagca ccaccccaag guccucacgg acgaggccua ccaccgccug      720

cacgagaucg uguuccggca gcgcccccug cgggccccga aggugggggc cugcacccuc      780

guccccggcg aggcccgccu gcccaaggcg cacccccugu uccagcggcg ccggcuccug      840

gaggagcuga acgcccucau gaucgugcgc gccggggccg uggccgagcg gcugaccccg      900

gagcagcgcg accugcuccu gcugaagcuc aaggacaagg gcaaggucac cuucaagagc      960

cugcgggaga aggugcugaa gcucgacggc gacgcgcgcu ucaacaagga guccgagcac     1020

cggacggagc ugaaggggga cgagguggcc gccgagaugg gcgggaagac ccgcuucggc     1080

agccuguggc cccaccucuc caccgagcag caguggaccg ucaucgagcg ccagcaggcc     1140

cuggagagcg acgcggacga ggccgccuuc cggacguggc ugguggagac ccacaagcuc     1200

accgccgagc aggcccgcgc gguggccggg gcccggcugc ccgccggcca cggccgcuuc     1260

gggcugaccg ccacggcggc ccugcucgcc gcccugcggg acggccgcac ccccgagggg     1320

cggggcccga acgcccccgc gaccgaggcc gggcgcgucg ugguguacuc cgaggccgcc     1380

gcgaucgccg gcuaccacca cagcgaccac cgguccggcg aggucuucac cgacgccaag     1440

gggcgccccg cccugcccua cuacggcgug ccgcucgacc ggcacaucgu gcccgggacg     1500

gccgaccccg acgagcccga cgaggcggcc cgcaucggcc ggcugaccaa cccgaccguc     1560

cacaucgccc ugaaccagcu ccgccgggug gugaaccgcc ugauccgggu cuacggcccg     1620

cccgccgagg uggcccugga gcucgcgcgc gagcugaagc ugagcgagga cgagaagaag     1680

gagcggaacc gcgagaacuc ccggaaccgc cucgacgccg agaagcgcag cagcaagcug     1740

gccgagcugg ggcagcggga caacggcggg aaccgcgccc uccugaagcu gugggaggag     1800

cucaaccccg agaacauccu ggaccggcgc ugcaucuacu ccggccggca gaucagcauc     1860

gggaugaucu ucuccggcgc cgcggagguc gaccacaucc ugcccuucga cgccacccuc     1920

gacgacagca acgccaacaa gauccugugc cugcgcgagg ccaaccggga caagcgcaag     1980

cggucccccu ucgaggccug gggcgggacg ccccaguggg aggagaucgc ggagcgcgcc     2040

agccggcucc cgcgcaacaa gcgguggcgc uucgagcccg acgccaugga gcgguucgcc     2100

gaggagggcg gguuccuggc gcgccaccug guggacaccc aguaccucgg ccggaucgcc     2160

cacgacuacc ugcgcugccu guaccccgac aagggggacg gcuccagcca cguguggguc     2220

ucccccggcc ggcucaccga gauggugcgc cgcaagcugg ggcugaacgg ccuccugggg     2280

gaccacaacc ugggcgccga gcagccgaag aaccggaagg accaccgcca ccacgccauc     2340

gacgccgugg ucaccgcgau ccucgaccgg agcaugcugc agcgcaucca gcgggccucc     2400

ggggagggcg ccagcgacgc cgagcugcuc cgcaucaucg ugcccgagcc cuggacgggc     2460

uuccgggacg accugcagcg cgccguggac cggaucgucg uggcgcaccg cgccgaccac     2520

gggaccgugg ccaaggccgc cacccggggc cgcgaccaga ccgcggcccg gcugcacaac     2580

gacacggccu acggguucac cggcgaggcc gacgcgaagg ggacccccau cgucgugcac     2640

cgcgccccgc ucggcgcccu gaagaagccc gagcacaucg accaggugcg ggacccccug     2700

cuccgcgccg cccugcacga cuucaccgcg ggccuguccg ggaagcccuu cgaggaccgg     2760

aucgccgccu ucccgcgccu cggcccccug gacuaccggg ggauccgccg gguccgcgug     2820

guggagcccc ugcaggucau ccccauccgc gacgccgccg gccgggcgua caagggcuac     2880

aagggggaca gcaacuaccg cuacgacgug ugggagcucc cggacggcaa gugggagcag     2940

cgggugcugc agauguacua cgcccaccag uccggggagc cgccccgccc ccaccccgcc     3000

gcccggaagg uccugagccu ccaccgcgac gacgugcugg ccaucgagcg gggcgagggc     3060

gggcgcgagc uggugcgggu cgugaaguuc uccacgaacg aguucacccu cgcgccccug     3120

aacgagggcg gggcccucaa gagccgccac gccgacaagg gcgaccccuu ccgguaccug     3180

uaccccuccc cgagcacccu gaaggccugg cgcgcgcggg aggugcgcgu cgacgagcug     3240

gggcgggugc ucgaccccgg cuuccccgcc cgcaagcggc gccgggugac ccgcccgggg     3300

cgcgccgacg ccgacuga                                                   3318


<210>  1599
<211>  3321
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1599
augcaguaca ucguggccau cgacaucggc gucgccagcg ugggguacgc ggugaucgac       60

aagaccuccg agaccgucau cgaggccggc agcaacaucu uccccgaggc cuccgccacg      120

gacaaccagg agcgccgggg gaugcgccag gcccggcgcc ugaagcggcg cgagcggacc      180

cgccucgacg acuucaccaa gcugugggag uaccacggcc uggcgauccc ccaggugaag      240

aagaacgaca ucguggggcu caaggucaag gcccuguacg agagcaucuc ccuggacgag      300

guguaccuca uccuguacag cuaccugaag caccggggca ucuccuaccu cgaggacgug      360

gaggacagcu ccgucagcgg cuccaccgcc uacgccaacg ggcugaagcu gaacgcgaag      420

gagcucgaga agaacuuccc gugcgagauc cagaaguacc gccuggagaa caucggcaag      480

uaccgggggc agauccagau cgaggacgac cagggcgaga agcuggaccu cagcaacgug      540

uucacgaucg gggccuacac caaggagauc cagcagaucc uggacaccca gaagaaguac      600

cacgccgaga ucgaggacga guucugcgag aaguaccuga agaucuucac ccgcaagcgg      660

aaguacuacg agggccccgg caacgagaag ucccgcacgg acuacgggaa guacaccacc      720

cggcucaagg agaacggcga guacaucacc gagaagaaca ucuucgagaa gcugaucggg      780

aagugcagcg uguacaagga cgagcugcgc gccgccuccg cgagcuacac ggcccaggag      840

uucaaccucc ugaacgaccu gaacaaccuc accgucaacg gccggaagcu ggaggagaac      900

gagaagcacc ugaucgugga caaggugaag accuccaaca ccaucaacau gcgcaagauc      960

aucgccgacg ccaugggcga gaagaucgag gaguucuacg gggcccggau cgacaagagc     1020

gagaaggaga ucuuccacaa guucgagguc uacaacaaga ugcgcaaggc gcucgccgcc     1080

auccacguga acaucgagga guucucccgg gacgaccugg acgagaucgg cuacauccug     1140

acgaucaaca ccgacaagga ggccaugaug gaggccuucg acaacagccg ccucgagcug     1200

cccggggaga ucaagaacug ccugaucagc cuccgcaaga ccaacggcac ccuguacucc     1260

aaguggcaca gcuucucccu gaagaucaug aacgagcuca ucccggagau guaccggcag     1320

cccaaggagc agaugacgcu gcugaccgag auggggguga ugaagggcca gaccgacgag     1380

uucgagaaga acaaguacau ccccgucgac guggcgagcg aggacaucuu caaccccgug     1440

guccgccggu ccgugcgcau cagcuucaag auccucaacg cccugaugaa gaaguacggc     1500

acccuggacg agguggucau cgagaugccg cgggaccgca acuccgacga gcagaagaag     1560

ugggagaagg agacgcagaa gaagaacgag aaggagcagg aguacaucga gaacaagcuc     1620

gccaccgugu acgacauccg gcugagcucc gccgacuuca gcucccagaa gcagcugaac     1680

cucaagcuga agcuguggaa cgagcaggac gggaagugcc ucuacagcgg caagaucauc     1740

gagcccgagg accugaucaa gcacuccgag cuguucgaga ucgaccacau caucccccgc     1800

agcaucuccu ucgacgacag ccgguccaac aagguccugg uguaccgcac cgagaaccag     1860

aacaagggga accagacccc cuacuacuac cucacgcaca ccaacagcaa gugguccuuc     1920

gagcaguaca aggcccuggu gauggaccug agcaagaaga aggaguacgg ccucagcaag     1980

aagaaggucc ggaaccugcu guacuccgag gacaucacca aggcggacgu gcuccgcggg     2040

uucaucaacc ggaacaucaa cgacaccagc uacgccgccc gcgugguccu gaacacggug     2100

cagggcuacu uccgggccaa ggagaugggc accaagguga aggucauccg cggguccuac     2160

acccaccaga ugcgggugaa caugaagcug gacaaggacc gcgagaaguc cuacgcgcac     2220

cacgccgugg acgccaugcu caucgccuuc agccagcugg gcuacgaggc cuaccggaag     2280

cugcaggggg aguucaucga cuucgagacc ggcgagaucc ucaacagcga gaaguggaag     2340

gaccagaugu ccgacgaggu cuacaagcag uaccuguacc gcaagaagug gagcaacauc     2400

cgcaacgaga ucacgcgggc ggagaagaac gugaaguacu gguacuacgu ggaccacaag     2460

uccaaccgcg ggcugugcaa ccagaagauc cggggcaccc gcgucuacga cggcaagauc     2520

uacaagauca acaagcucga cauccggaag aaggaggggg ccgcccuguu caagaagcug     2580

gccuucagcg agaaggaguc cgaccgcgag aagcuccugg uguacaagaa cgacaagaag     2640

accuacgagg accuggugcg gaucaugcag gacuacagcg acuccaagaa cccguucguc     2700

caguacgaga aggagaccgg cgacuacgug cgcaaguacg ccaagaagca caacgggccc     2760

cggaucgaca agcucaagua caucgacggc gaggugggca gcugcaucga caucucccac     2820

aaguacgggu acgagaaggg cagccgcaag gucgugcugg agucccuggu gcccuaccgg     2880

auggacgucu acuacaagaa ggaggagcag accuacuacc ucguggggau caagcagagc     2940

gacgugaagu gccagaaggg cgaguacguc aucgacgagg acgcguacgc caucaaccug     3000

gugaaggaga agaugaucca gcaggggcag aagcgcagcg accuggaguc ccucggcuuc     3060

gaguuccagc ugagcuucua caagaacgac aucaucgagu acgagaagga cggcaagauc     3120

gugcgggagc gcuuccuguc ccggacgaug cccaagguca agaacuacau cgagaccaag     3180

ccgaucgacc gcgccaagua cgaguggcac gaccggaagc ucguggggcu gggcaagacc     3240

aagcgcaucc ggaaguaccg cauggacauc cuggggaacu acuaccucug caacaaggag     3300

gaguucagca aguacugcug a                                               3321


<210>  1600
<211>  3324
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1600
augggcuaca ccguggggcu ggacaucggc gucgccagcg uggggguggc cguccucgac       60

gagaacgaca acaucgugga ggcggugucc aacaucuucg acgaggccga caccagcaac      120

aacaaggucc gccggacgcu gcgcgagggc cggcgcacca agcggcgcca gaagacccgg      180

aucgaggacu ucaagcagcu gugggagacc uccggguaca ucauccccca caagcuccac      240

cugaacauca ucgagcugcg caacaagggc cucacggagc ugcugagccu cgacgagcug      300

uacugcgugc ugcucuccau gcugaagcac cggggcauca gcuaccugga ggacgccgac      360

gacggggaga agggcaacgc cuacaagaag gggcucgccu ucaacgagaa gcagcugaag      420

gagaagaugc cgugcgagau ccagcuggag cgcaugaaga aguacggcaa guaccacggg      480

gaguucauca ucgagaucaa cgacgagaag gaguaccagu ccaacguguu caccaccaag      540

gcguacaaga aggagcucga gaagaucuuc gagacccagc ggugcaacgg caacaagauc      600

aacacgaagu ucaucaagaa guacauggag aucuacgagc gcaagcggga guacuacauc      660

ggccccggga acgagaaguc ccgcaccgac uacggcaucu acaccacccg gacggacgag      720

gaggggaacu ucaucgacga gaagaacauc uucggcaagc ugaucggcaa gugcagcguc      780

uaccccgagg aguaccgcgc cagcuccgcc agcuacaccg cccaggaguu caaccugcuc      840

aacgaccuga acaaccugaa gaucaacaac gagaagcuca ccgaguucca gaagaaggag      900

aucguggaga ucaucaagga cgcguccagc gugaacaugc ggaagaucau caagaagguc      960

aucgacgagg acaucgagca guacuccggg gcccgcaucg acaagaaggg caaggagauc     1020

uaccacaccu ucgagaucua ccggaagcug aagaaggagc ugaagacgau caacguggac     1080

aucgacagcu ucacccgcga ggagcucgac aagaccaugg acauccugac ccugaacacg     1140

gagcgggagu ccaucgugaa ggccuucgac gagcagaagu ucgucuacga ggagaaccuc     1200

aucaagaagc ugaucgaguu ccgcaagaac aaccagcgcc uguucagcgg guggcacucc     1260

uucagcuaca aggccaugcu ccagcugauc cccgugaugu acaaggagcc gaaggagcag     1320

augcagcugc ucaccgagau gaacguguuc aagagcaaga aggagaagua cgucaacuac     1380

aaguacaucc ccgagaacga gguggugaag gagaucuaca accccgucgu ggugaagucc     1440

auccggacca ccgucaagau ccugaacgcc cugaucaaga aguacggcua ccccgagagc     1500

guggucaucg agaugccgcg cgacaagaac uccgacgacg agaaggagaa gaucgacaug     1560

aaccagaaga agaaccagga ggaguacgag aagauccuca acaagaucua cgacgagaag     1620

gggaucgaga ucacgaacaa ggacuacaag aagcagaaga agcuggugcu gaagcucaag     1680

cuguggaacg agcaggaggg ccugugccuc uacagcggca agaagaucgc gaucgaggac     1740

cugcugaacc accccgaguu cuucgagauc gaccacauca uccccaaguc caucagccuc     1800

gacgacuccc ggagcaacaa ggugcugguc uacaagaccg agaacuccau caaggagaac     1860

gacacccccu accacuaccu gacccgcauc aacgggaagu ggggcuucga cgaguacaag     1920

gccaacgugc uggagcuccg gcgccggggg aagaucgacg acaagaaggu gaacaaccug     1980

cugugcaugg aggacaucac gaagaucgac gucgugaagg gcuucaucaa ccgcaaccuc     2040

aacgacaccc gguacgccag ccgcgugguc cugaacgaga ugcaguccuu cuucgagagc     2100

cggaaguacu gcaacaccaa ggugaaggug auccgcgggu cccugaccua ccagaugcgg     2160

caggaccucc accugaagaa gaaccgcgag gagagcuacu cccaccacgc cgucgacgcc     2220

augcugaucg cguucagcca gaagggcuac gaggccuacc ggaagaucca gaaggacugc     2280

uacgacuucg agacgggcga gauccucgac aaggagaagu ggaacaagua caucgacgac     2340

gacgaguucg acgacauccu guacaaggag cgcaugaacg agauccggaa gaagaucauc     2400

gaggccgagg agaaggugaa guacaacuac aagaucgaca agaagugcaa ccgcgggcug     2460

ugcaaccaga ccaucuacgg cacccgggag aaggacggga agauccacaa gaucuccagc     2520

uacaacaucu acgacgacaa ggagugcaac ucccucaaga agaugaucaa cagcggcaag     2580

gggagcgacc ugcugaugua caacaacgac ccgaagaccu accgcgacau gcucaagauc     2640

cuggagacgu acuccagcga gaagaacccc uucguggccu acaacaagga gaccggcgac     2700

uacuuccgca aguacuccaa gaaccacaac ggccccaagg ucgagaaggu gaaguacuac     2760

agcgggcaga ucaacuccug caucgacauc agccacaagu acggccacgc caagaacucc     2820

aagaaggugg uccuggugag ccucaacccc uaccggaccg acguguacua cgacaacgac     2880

accgggaagu acuaccuggu cggcgugaag uacaaccaca ucaagugcgu gggcaacaag     2940

uacgucaucg acuccgagac cuacaacgag cugcuccgca aggagggggu gcugaacagc     3000

gacgagaacc uggaggaccu caacuccaag aacaucacgu acaaguucag ccuguacaag     3060

aacgacauca uccaguacga gaagggcggg gaguacuaca ccgagcgguu ccugucccgc     3120

aucaaggagc agaagaaccu caucgagacc aagccgauca acaagcccaa cuuccagcgg     3180

aagaacaaga agggcgagug ggagaacacc cgcaaccaga ucgcgcuggc caagacgaag     3240

uacgugggga agcuggucac cgacgugcuc ggcaacugcu acaucgugaa cauggagaag     3300

uucagccugg ucguggacaa guga                                            3324


<210>  1601
<211>  3324
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1601
augggcuaca cccuggggcu cgacaucggc aucgccagcg ugggguucgg ccugaucgac       60

aacgucgacg ggaagauccu ggaguccggc gugcgccucu ucagcuccgc cgaccccgac      120

aacaaccaga cccggcgcag cucccggcag gcgcgccggc ugaaccgccg gaaggugcac      180

cgccuggagc gggucgccca ccuccuggag gaggccggcu ucgagaaggg ggaggugcug      240

gccccgacgc ccuaccaccu ccgcgugaag ggccugaccg agaagcugag caagcccgag      300

gucuacgccg cgcucuacaa ccugaugaag caccggggga ucuccuaccu ggaggacgug      360

gagcucgacg aggccgacag cuccagcgug ucccagaacg ucgagcugcu gaaggaguac      420

uaccccugcg agauccagcu cgagcgcuac gaggaguacg gccagauccg ggggaucauc      480

gagaccuaca acaaggacgg cgagaaggag agccugguga acaucuuccc gaccggcgcc      540

uacaagaagg aggccgaggc gaucauccug aagcaggggc aguucuaccc cgagcucgac      600

aagguguucc uggagaaguu cacggagauc cugaccguca agcgccccua cuacgugggc      660

cccgggaaca agaagucccg gaccgacuac ggcaucuacc gcgagaacgg cgagacccuc      720

gagaaccugu ucgacauccu gaucgggaag ugcacgaucu acccgcagga ggcccgggcc      780

gccaaguuca gcuacaccgc ccagcuguuc gacuuccuga acgacaugaa caacaucucc      840

aucgcgggcg agaagcugac cgaggugcag aagcgcgaga ucuaccacca gaucaugagc      900

uccaagaccg ucaacaugau gaagaucauc acgaagguga ccgggugccu caaggaggag      960

gugaccggcu accgggucga cagcaaguac aaggccgagu uccacuccuu cgccagcuac     1020

cgccaguucc ggaacaagau gcuggacuac ggguucgacg ugcagacccu gaacguggag     1080

auguucgaca agcucgccgc caagcugacg cugaacaccg aguaccacca gaucaucacc     1140

cagcucgaga gccugaaccu gggccucucc aaggagcuga ucgagaagau cgcggacuuc     1200

cgcaagaaga accgcagccu guucuccaag uggcacagcc ucucccugaa ggccaugaac     1260

gaggucgugg acgagcugug gagcaccucc aagaaccaga ugcagaucuu cagcgagcuc     1320

ggcggguuca aguccaacau cgagguguuc aagggccuga aguacauccc ccuggacgcc     1380

auggucgacg agaucuacaa ccccgugguc cggugcagca uccgcgaguc caucaagauc     1440

aucaacgccc uccugaagaa guacggggcc cuggacaagg uggugaucga gaugccccgg     1500

gacagcaacg agaaggagga gaaggaccgc aucacgaagc ugaacaagga gaacgacaac     1560

aagaagaagc aggcgaucga gaaggcccgg aacgaguacg gcuucgggga ggaggccuac     1620

cgccagcaga acgaccucaa caccaagcug cggcuguggu acgagcagaa gcagcgcugc     1680

cucuacuccg gcgagaucau caagaucuuc gaccugauca agaacccgca ccuguucgac     1740

aucgaccaca ucauccccaa gagcaucucc uacgacgaca gccucuccaa caagguccug     1800

ugcuacgcca ccgagaaccg ggagaagggc aaccugaccc ccuaccgcua cuucaagcgg     1860

aaggccagcc ucacguggaa cuacgaggcg uacaaguccu acgugaucga gcuguacaag     1920

gacgggaagg gcgaccugag ccgcaccaag cucgagcugc uccucuucga ggaggacauc     1980

aacaaguacg aggugcggca gcgcuucauc aaccggaacc uggucgacac ccgcuacgcc     2040

agcaaggugg ugcugaacac ccuccaggac uucaugaagg agaacuaccc cacgaccacc     2100

gucgaggugg ugcgggggaa guucaccgcc cagcugcgcc ggcacugggg cauccgcaag     2160

aaccgcgacg agaaccacgc ccaccacgcg aucgacgccc ugaucaucgc cugcgucccg     2220

caccucuccc uguggaagga gcagaagaag guguucagca cggacgaguu ccggguggac     2280

gagaccaccg gggagaucau cgagcagucc uacaaccagc ugaccuacca ggagcccuuc     2340

agccaguucu ucuaccagcu cgagaagguc uccaucgacu gccgcuacag cuacaaggug     2400

gaccggaagu ucaaccgcca gguggccgac gccacgaucu acuccacccg gcagggcauc     2460

gucaagcaga ccagcggcga caaguccuuc acccuggaca gcaacuccaa ggagcacuac     2520

gugaucgcga agaucaagaa caucuacgag gacguggacg ccaagcgcuu cauggaccgg     2580

uacaagaagg acaagaccca guuccugaug uaccaccacg acccccagac guucagcauc     2640

cucgaggacg ucaugaagac cuaccccgac aagaagaacc cguuccaggc cuaccgccug     2700

gaguacgggc ccauccggaa guacuccaag aagggcaacg ggccccagau ccagagcauc     2760

aaguacuacg acuccaagcu gggccagcac aucgagcuca agcaggccaa ccagagcacc     2820

aagaaggugg ugcugcaguc ccugaacccc uggcgcugcg acaucuacua caacaagaag     2880

gacaagcggu acaucgccgc gggccucaag uacgccgacc ugagcuacca gaaggggacc     2940

ggccuguacg ggaucagccu ccagcgcuac cagcagaucc ugaacuccga gaagaucgag     3000

cugagcaucg agcagaucga gcuccugcug gagggccggc ucaacgaguc caacuucgag     3060

uucugcuuca gccuguacaa gaacgacuuc auccagugga acgacgagaa caaccagacg     3120

uuccaguacc gcuuccuggc ccggaaccuc uccagcccga accgcaucga ggucaagccc     3180

aucaacaagu ccaaguacga caagcaggcc gaggggcuga agacccugaa gaagggccuc     3240

aaccgguucu acaagaucag cguggacgug cugggcuacc agcacuucau caagaaggag     3300

aagcugaagc ucaccuucaa guga                                            3324


<210>  1602
<211>  3330
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1602
augagccacu acgugcuggg ccucgacauc gggaucaccu ccgucggcua cgggaucauc       60

gaccuggaca ccaacgacuu caucgacuac ggcgugcgcc uguucaagga ggggacggcc      120

gccaacaacg aggaccggcg caacagccgg ucccgccggc gccuccugag ccggaagcac      180

acccgccuga uggacaugca gaaggugcuc aaggaggcgg gcaucaugac cgaguccuac      240

agcccccugu ccaacgucua cgagauccgg aagaagggcc ugaccaagga gcucacgaac      300

gacgagcuga ccgccgccau ccugcacauc accaagcacc gcgggagcgu gaucgagacc      360

guggaggacg acgaggccgc cgcgaaggag acgggcgagc ucaaguccac ccugcacgag      420

aacgcccugc uccuggccaa caacaaguuc gucugcgaga uccagcugga gcggcucaac      480

accgagaaca agauccgcaa ccacaagaac aacuucaaga ccaaggacua cguggccgag      540

cugaaggaga uccugaacca ccagaaccuc agcgaggagg ugaagaacaa gaucauccag      600

aucgucgagc ggcgccgggc guacuacgag gggccgggcu ccuucaacag ccccacgccc      660

uacgggcgcu ucaucgaggu gaacggccag gugcaggaga ucgaccugau cgagaagaug      720

cggggcaagu gcuccgucua ccccgacgag uuccgcgccc cgaagaugag caucuccgcc      780

gaccuguuca acauccucaa cgacuucaac aaccugcugg ugcaggggga gaagcuccag      840

gaggaggaca agaagaaccu gcugaagauc aucaacgaca agggcagcau cacccuccgg      900

cagaucucca agaagcugga ggugaacgag gacgagauca agggguaccg caucgacaag      960

aagggcaagg cccuguucac cgaguucaag ggcuacaaga uccucaagaa gcuguuccug     1020

aacugcgggc agagcaucuc ccucgaggac uacgagaugc uggacgagau caucgagauc     1080

gucaccaaga agaagggcau cgacgagcgg aaggacgccc ugaaggagcu caagugggac     1140

cugagcgacg acaucaucaa cagccugucc gaggugacgg ggaucguggg cuaccacagc     1200

cucucccuga aggcgauccg cgagcuggac agcgagcucu acaagaccuc ccugaaccag     1260

augcagcugc uccacgagcu gaagauguuc gacaagaacc gccccagcua caaggggaag     1320

aagaacaucg ucgccgaccc cgaggccauc cugucccccg uggccaagcg ggcccagaac     1380

gagaccuuca aggugaucaa cgcgcugcgc aagaaguacg gcgaguucga cagcaucguc     1440

guggagauga cccgggacaa gaacucccag gagcagaaga agcgcaucaa cgacagccag     1500

aaggcccucg agucccugaa caaggaggug gaccagcugc uccagcagaa gggcuacgac     1560

ccgcagaagg ucaaggggaa gacgaagcag aaggugcggc uguacaugau gcaggagggc     1620

aagagcgccu acacccugca ggagcucgac cugcaccgcg ucaucaagga cgacaaguac     1680

accgagaucg accacaucau ccccaucucc aucagccugg acgacuccca gagcaacaag     1740

gugcucgccc ugcacuccga gaaccagcag aaggggaacc ugacccccuu cauggccuuc     1800

cacacgggcc gguucgcgga cauggggugc accuggaaca aguucaagga gaacgugcuc     1860

agcaacaaga acuacaagca caagaagaag aacaaccugc uggacgagcg cgacaucaac     1920

aaguucuccg ucauccagga guucaucaac cggaaccucg uggacaccag cuacgccaac     1980

cgcguggucc ugaacacccu gacggacuac uucaaggaca acgagaucga caccaaggug     2040

cacaccguga acggcaagcu caccgaccug uuccggaagc gcaucaaccu gaagaaggac     2100

cggaacgagg acuaccucca ccacgccauc gacgcccuga ucgucgccag caucaagaag     2160

cugcgccucc uggacggcua ccuggcgaag uacggguucg agacgcucua cgacgagaug     2220

accggcgaga ucaaggagau ccccgaggac aaggccuucu ucgacgacaa guacauccgg     2280

uacauccuga accugaagac caucuacgag uccagcaaca uguacuaccg cgggauccuc     2340

gagcgggagg agcugaucua ccagccgauc aagaucuccc acaagaucga caccaagccc     2400

aaccgcaagg ugagcgacga gacgaucuac uccacccgga ucaucgacaa cgaggaccac     2460

cugaucgaga aguacagcga caucuacgac cccaaguuca agaaccucac cgaggacauc     2520

cugaacggcg agaagaacaa guaccugaug gagaagaacg acccccagac cuucaacauc     2580

cucaagcaga ucaucuucaa ccacuacaac accuacaagg acuccaagga guacuacaag     2640

aucgagaaga agaaggagaa ggagaucauc aagcuggugg ggaucaaccc gcugagccuc     2700

uacaaggagg agaacggccc caucaagaag uacuccaaga agggcaacgg gcccgucauc     2760

augagcauga aguacgacuc cgagaagcug ggcaaccaca ucgacaucac gcccgacagc     2820

gccaagaaca agaaggugau ccugaagcag aucuccccgu accgcaccga cuucuacaag     2880

ugcagcgacg agaaguuccg cuucgugacc guccgguaca aggacgugca cuaccagaag     2940

uccaucgaca aguacugcau cgacaaggag ugguaccaga acgagaagaa gaacaagaag     3000

aucgacgaga aggccgaguu ccucuucagc augcaccacg acgagcugau cgggaucacc     3060

cgcaaggagu ccgagcaguu caucuacgac gagagcaagg agaaccacgg cgacccccgg     3120

uaccacgacg gcgugaagcc cgagauccug aaguucacgg cgaccaacaa cgacaccaag     3180

gggaucaucg aggucaagcc ccucuacacc uacagcgccu accgccugaa gccgacggug     3240

cugaacguga agaacaucaa gaaguacgcc accgacgucc ucggcaaccu guacgaggug     3300

aagcagaacg agcugaagcu cgaguucuga                                      3330


<210>  1603
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1603
auggccgagg gcaagaagcg caucuggcgg cugggggccg accucggcgc gaacagccug       60

gggugggccg ccaucgagcu guccgccccc gugaacggca agccgaucgc cauccucgcg      120

gccgggagcc gcaucuucuc cgacggccgg gaccccaaga gcggcgccuc ccuggccguc      180

gaccgccggg acgcgcgcgc caugcggcgc cggcgcgacc gguucaagca gcgccagaag      240

gcccugauca aguaccucau cgccgacggg cuguuccccg ccgacgaggc ggcccggaac      300

gccauggccg ccaucgaccc cuacgcggug cgcgcccggg cccuggacga ggcccucagc      360

cugccggagc ugggccgcgc ccucuuccac cugaaccagc ggcgcggguu caaguccaac      420

cggaaggcgg accgcaaggc cgacgacgac gccggcaaga ucgccaucgg gaucgaccgg      480

acccgcgacg ccaucgcgga ggacgaggcc gacgccggcg ccgagcccgg cagcuggacc      540

uucgggaagu ggcugcacga gcgccggcuc aagggccagu ccgugcgcac gcggcugcgc      600

cccgagagcg gggaggacgc gaagggcgac ggcuacgacu ucuaccccga ccgggcccug      660

auggaggagg aguucgacgc caucugggcc gcccaggcgc cgcaccaccc cgacguccuc      720

accaacgacg ugcacgaccg ccugcacgag aucaucuucu accagcggcc ccugaaggcg      780

ccccaggugg ggaugugcuc ccucaucccg ggcgaccgcc ggcugcccaa ggcccacccc      840

cuguuccagc gccggcgccu ccuggaggag cugaacgccc ucaugaucgu ccggcccggg      900

gagguggccc agcgccugac ccgggaggag cgcgacaucc ugcuccugaa gcugaaggac      960

aagaacaagg ugagcuucgc cacccuccgg aaggcccuga agcuggaccc ggacgcccgc     1020

uucaacaagg aguccgagga ccggacggac cucaagggcg acgaggucgc cgccgugaug     1080

gggagcaaga cccgcuucgg caaccugugg gugcaccugu ccaucgaggc gcagugggac     1140

guccucgccc gccuggacgc cguggagagc gacgccgagg acgcggccuu cugcgccugg     1200

cugguggaga ccuacgcccu cacccccgag caggcccggg cggucgccaa cgcgccccug     1260

cccccgggcu acgggcgcuu cggcgagacg gccacccgga agcugaucga ggcccuccgc     1320

gacgggaaga aggacgaccg ggugcuggug uacuccgagg ccgucaccga ggccggccug     1380

gggcaccaca gcgaccgccg gaccggcgag guguucgagg acgccaaggg ccgcccccag     1440

cuccccuacu acgggguggc ccuggagcgg cacaucaugc ccggcacggg ggacccgucc     1500

gaccccgagg agaugcgcgu cggccggcug accaacccca ccgugcacau cgggcugaac     1560

cagcuccgcc ggauccugaa cgugcugauc cgcgccuacg ggcccccggc ggagaucgcc     1620

aucgagcucg cccgggagcu gaagcugacc gacgaggaca agaagcgcau caaccgggag     1680

aacaacgaca accgcaucgc cgcggagcgg cgcagcgaga agcuccuggc caucggccag     1740

gccgacagcg gcgccaaccg ggcccugcuc aagcuguggg aggagcugaa ccccgcgaac     1800

guccucgacc gccgcugcau cuacuccggc cggcagauca gcaucgacau gcuguucucc     1860

gccgccgugg aggucgacca cauccugccc uucgccgcca cgcucgacga cagcaacggg     1920

aacaagaucg ugugccugcg cgaggcgaac cgggagaagc gcaagcgguc cccgcacgac     1980

gccuggggcc acagcgcccg cugggacgag aucgccgccc gggcggccca gcugccccgc     2040

aacaagcggu ggcgcuucga gcccgacgcc auggacaagu uccgggacga gggcggguuc     2100

caggcccgcc accucgugga cacccaguac cuggcccggc ugucccgcga guaccucgcg     2160

acccuguacc ccgagaaggg cgaggggagc ggccacaucu gggucucccc ggggcggcug     2220

accgagaugg ugcgccggaa gcucggccug aacgaccugc uccccgacca caacuucggc     2280

gggggcgccg accaggccaa gaaccgccug gaccaccggc accacgccau cgacgcggug     2340

gucguggcca ucguggaccg cgggaugcug aacgccaucg cccgcgccag cggccaggag     2400

ggggcggagg gccgggagcg caucaucauc cccgagcccu gggacggcuu ccgggacgcc     2460

cuccgcgaga cggucaaccg gaucaccgug ucccaccgca ccgaccacgg gaccgccagc     2520

aaggccggcc ugccgaaggg gaaggacgcc acggcgggcc ggcugcacaa cgacaccgcc     2580

uacggguuca ccggcgacac cgacgccaag ggcaacucca ucgugguccg ccgggugccc     2640

cucagcuccu ucaagaagcu ggccgaccug gaccgcaucc gggacgccga ccucaaggcg     2700

gcccuguacc agcaccugaa cgggcucgag ggcaaggccu ucgagaaggc ccuggcggag     2760

uuccacaagc uggaguggca caacuucggg ggcgugcgcc ggguccgcgu gacggagccc     2820

cucaccguga uccccaucaa ggaccggaac gggcgcaucu acaagggcua caagggcgac     2880

agcaacuacc gguacgacgu cugggagcug ccggacggga agugggugac caacuggaag     2940

aacgaggccg gcgaggccau guccagcgug aucuccaugu ucgacgccca ccagagcagc     3000

gagccgcccc gcccccaccc cgccgcgcgg aagauccugu cccuccacca gaacgaccug     3060

cuggccaucg agcgcgaggg ggaggaccgg aagcucaugc gcgucgugaa guucggccag     3120

agcggccaga ucacccuggc cgagcaccag caggccgggg accugaagaa gcgcgacgcc     3180

guguccaacg acgacgaccc cuucaaguac uucgcgccca cgagcggcgg gcucaagaag     3240

gucaaggccc gccaggugcg gaucgacgag aucggccggg ugcuggaccc cggguucccg     3300

gcccgcaccg cccggcgcaa gacccggggc uga                                  3333


<210>  1604
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1604
augaccgccc gcacccacaa gaucuggcgg cugggcgccg accucggggc gaacagccug       60

ggcugggccg ccaucgagcu guccgccccc guggacggga agccgaucgc cauccuccac      120

gcgggcagcc gcaucuucuc cgacgggcgg gaccccaaga gcggcgccuc ccuggccguc      180

gaccgccggg acgcccgcgc gaugcggcgc cggcgcgacc ggcugcagca gcgccagcgg      240

gcccucauca aguaccugau cgccgacggc cuguuccccg ccgacgagaa cgagcgcaag      300

gccauggcga cgaucgaccc cuacgccgug cgggcccgcg cccucgacga ggcccugacc      360

cugccggagg uggggcgggc ccucuuccac cugaaccagc gccggggcuu caagagcaac      420

cgcaaggccg accggaaggc cgacgaggac gccgggaaga ucgccaucgg caucgaccgc      480

acccgcgacg cgauccggca ggccgaggag gaggccgggg ccgagcccgg cuccuggacc      540

uacggcaagu ggcuguacga gcgccgggcc agcgcgacgg acgccaacgc cauccccucc      600

guccgcaccc ggcuccgccc cgagaccgac gagcacgcca agggggacgg cuacgacuuc      660

uacccggggc gggcgcugau ggaggccgag uucgccgcca ucugggaggc ccaggcggcc      720

caccaccccg acgcccugac caacgaggug cacgaccgcc uccacgagau caucuucuac      780

cagcggcccc ugaaggcgcc ccagaucggc gugugcacgc ugauccgcgg cgaccagcgg      840

cucccgaagg cccacccccu guuccagaag cgccggcugc ucgaggagcu gaacgcgcug      900

accaucgucc gccccgggga gauggcccag cggcucaccc gcgaggagcg ggacacccug      960

uuccugaagc ucaaggacaa ggccaaggug agcuucgugu cccugcgcaa gacgcugaag     1020

cucgaccccg acgcccgguu caacaaggag agcgagaacc gcuccgaccu gaagggcgac     1080

gaggucgccg cggccauggg ggccaagacc cgcuucggca accggugggu gcaccugagc     1140

aucgaggagc agugggacgu gaucgcccgc cucgaggagg ucgaguccga cgcggaggac     1200

gccgccugcc gggccuggcu ggccgcgacc uucgaccuga ccccggagca gauccgcgcc     1260

gugacgaacg cgccccuccc gcccggguac agccgguucg gcaagguggc cacccagcgc     1320

cugaucgacg cccugcggga cggcgagaag gacgggcgcg uccucgugua cuccgaggcc     1380

guggccgccg ccggccuggg gcaccacagc gaccugcgga ccggcgagau cuucgaggac     1440

gcccaggggc gcccccagcu ccccuacuac ggcgucgcgc uggagcggca caucaugccc     1500

ggcaccggga accccgacca cuccgacgag augcgcaucg gccggcugac gaacccgacc     1560

gugcacaucg ggcucaacca gcugcgccgg aucgugaaca cccugauccg cgccuacggc     1620

ccgcccgccg agaucgccau cgagcuggcg cgggagcuca agcugaccga cgacgacaag     1680

aagcgcauca acaaggagaa caacgagaac cggcuggccg ccgagaagcg cagcgagaag     1740

cuccugacga ucgggcggcc cgacaccggc gccaaccgcg cccugcucaa gcugugggag     1800

gagcugaacc ccgcgaacgu ccucgaccgc cggugcaucu acaccggcac ccagaucagc     1860

guggacaugc uguucuccgc cgccguggag gucgaccaca uccugcccuu cacggccacc     1920

cucgacgaca gcaacgggaa caagaucgug ugcaugcgcg aggccaaccg gaccaagcgc     1980

aagcaguccc ccuacgaggc guggggccac accgaccggu gggaggagau cgccgcccgc     2040

gccgcccagc ugccgcggaa caagcgcugg cgguucgagc ccgacgcgau ggagaaguuc     2100

aaggacgaga gcggguucca ggcccgccac cuggucgaca cgcaguaccu cgcccggcug     2160

ucccgcgagu accuggagac ccucuacccc ggcagcggcg agggguccag ccacaucugg     2220

gugucccccg gccggcugac cgagauggug cgccggaagc uggggcucaa cgaccugcug     2280

ccggaccaca acuucggcgg gggcgccgac caggccaaga accgccucga ccaccggcac     2340

cacgcgaucg acgccgucgu gguggccauc gucgaccgca gcaugcugac cgagaucucc     2400

cgggugagcg gccuggaggg ggccgagggc cgcgagcgca ucaucauccc gccgcccugg     2460

gacgaguucc ggaacgaccu ccgcacggcg gugaacgcca ucaccgucuc ccaccggacc     2520

gaccacggga ccaucagcaa ggccggccug cccaagggga aggacgccac cgccggccgc     2580

cugcacaacg acacggcgua cggcuucacc ggggagaccg acgccaaggg caacuccauc     2640

guggugcggc gcgucccccu cgccagcuuc aagaagcugg ccgaccugga ccggauccgc     2700

gacaccgacc ucaaggccgc gcuguaccag caccuggagg ggcucgaggg caaggccuuc     2760

gagaaggccg ugauggacuu ccccaaccug gaguggcacc ccuucgggaa cgugcggcgc     2820

guccggguga cggaggugcu guccgucauc cccaucagcg accagaacgg ccgcaucuac     2880

aagggcuaca agggggacuc caacuaccgg uacgacgugu gggagcuccc cggcgggaag     2940

ugggaggccg agguggucag caccuucgac gcccaccagc ccgacugggu gagcaagauc     3000

cgcucccgga gccccaccgc gcgcaaggug cugucccugc agcagaacga ccucaucgcc     3060

gucgagcggg cccacgagac ccgcgagcug augcgggugg ugaaguuccg ccagaacggc     3120

gagaucacgu ucgccccgcc ccaggaggcg gggagccuga agcggcgcga cgccaccccc     3180

aacgaggacg accccuucaa guacuucgcc gucgccgccu ccagccucca gaaggcgaag     3240

gcccgccagg ugcggaucga cgagcugggc cgcgugcugg acccgggcuu ccccgcccgg     3300

accgcccgcc ggaagacccg caaguccacg uga                                  3333


<210>  1605
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1605
augcgcaucc ugggcuucga caucgggauc accagcaucg gcugggccua cguggagggg       60

gaggagcuca aggacugcgg cguccggauc uucaccaagg ccgagaaccc ccagaacggg      120

gacucccugg cggccccgcg ccgggaggcc cgcggcgugc ggcgccggcu ggcccgccgg      180

aaggugcgcc ucaacgucau caagcggcug cugugcaagg aguucggccu caaccugagc      240

gacuacuucg ccaacgacgg ggagcugccc aaggcguacg agacgaccaa ggacaccaag      300

ucccccuacg agcuccgcag ccuggcccug gagcagaagc ucgagcccaa ggagcuggcc      360

cgggugaucc ugcacaucgc caagcaccgc ggcuacggga acaagcacgc gaagcgggac      420

cucgaggccg aggagaaggc caagaaggcc gccgagaagg cgccgcagga gaaggagucc      480

accacgacca acggcgacag caagaaggag caggagaagg ugcugaaggc ccuguaccag      540

aacgagaccc uccugaagaa guaccagacc aacuaccaga cgaacaacaa gaccaacgac      600

gagaccaaca acaagaccau cuuccugguc gggaaguacc ucuacaagga guuccaggac      660

aagggccagc gcauccggaa cacgaccaac aacuaccagc acaccaugcg ccaggagugg      720

cugaaggacg agcuggaguu caucuucaag aagcagaagg acuucggcgc caccuucucc      780

gagaacuucg agagccagau ccucgagacg gccuucuacc agcgggaccu gaaguccuuc      840

gaggacaagg uggggaagug cguguucuac gagaacgaga accgcgcccc caaggacagc      900

cuguccgcga uggaguucgu cgcccucacc cggaucauca acacccugaa gaaccuggag      960

aagaagucca agaaccucgg caucggggag accuacggca aggacaagau ccaggagauc     1020

cugaagaucg ugcuggacaa gggcgagaug agcuacaagg agcuccgcaa gauccugaag     1080

cugaacgaga gcauccucuu cgccaaggac uccaagcugg acuacacgaa ggggaugcag     1140

gaggccgaga aggccaaguu caucgagcug aagaaccuca aggcguucaa gaaggccaug     1200

ggcggggacu ucagcaaguu cccccgcgag gagcuggaca agaucgccac cgacaucgug     1260

cugaucaagu ccaaggagaa ccucgccaag aagcugcaga acuacagcgc ccuguccaag     1320

gagcaggucg aggcgcucag caaccugaac uucgccaagc acaucgaccu gagccucaag     1380

gcccuggaca agauccugcc cuucaugcgg gagggccugu ccuacgacga gguggugaag     1440

aaggucgggc uccaggagca ccgcaaguac aagcagaagg gcgaguuccu gaucccgcug     1500

aaggaguacg agcccuaccu cgccaacccc gugguggcgc gggcccugag cgaguaccgc     1560

aagguccuga acgcccuccu gcggaaguac ggcaacgugc acaagaucca ccuggaguuc     1620

acccgcgagg ccgggaagaa cuacgacgag cggaagaaga ucgagaagga gcagcgcgag     1680

cacuucgagg ccaaccagaa ggcgaucaag cagcgggaga uccucggccu gcccaucaac     1740

ggggccaaca uccugaagau gaagcucugg aucgagcagg gcgagugcug cguguacucc     1800

ggggagaaga ucaccagcaa gcacuucuuc gacuccaacg cccugcaggu cgaccacauc     1860

uacccguaca gccgcuccuu cgacgacagc uacaugaaca aggugcuggu guucgccaag     1920

gagaaccaga acaagcucaa caagacgccc uucgaggccu ucggcuccga caaggagaag     1980

ugggaccgga uccugagccu guccgcgaag cuccccaagc agaagcagcg ccggaucagc     2040

aacaaggacu ucaaggacaa ggagcugggc uucaucgccc gcaacaucaa cgacaccggg     2100

uacaucgccc ggcuggccuc ccaguggacc aacaacugcc ucaaguucga ggaccugcgc     2160

gagaacgagg ucaccaucgc cggcgagaag gggagcaaga accaccugga ggugaucucc     2220

ggccagcuca cgagcaugcu gcggcacuac ugggggcugg gcgacaagga ccgcaacaac     2280

caccuccacc acgcggucga cgccgugauc aucgccuaca ccaacgccaa gaccaucaag     2340

gcguucgccg acuucaagaa gaaccaggag cagaaccggg ccaaguucua cgccaagcag     2400

aucuccgagc aggaguacaa gcgccagcgc aaccccuucg agccgugcgc caacuuccgg     2460

cagcgcgugg aggagaagau caacaagauc uucgucagca agccgccccg gaagcgcgcg     2520

cggggcgccc ugcacaagga gaccuucggg uccaucugcc uggacggcaa caacaacugg     2580

guggcccgcu ucaagaucaa gagcaagaag gagcaggagg acaucacgaa ggagaaggcc     2640

accaaggcca aguacaagga cgaguucaag ggggaggaga acauccggaa ggcgcucaag     2700

cugggcaaga uccgccggau caacaacacc cagaaggaca ucgccaucgg cgacaagauc     2760

gugaagaacg agaccauggu ccgcguggac aucuucaagg acaagaaggg gaaguucuac     2820

gccgugccca ucuacacgau ggacuucgcc cugggcaucc uccccaacaa ggccgucgcg     2880

accggggagg acgaggacgg cgugaucaag gacuggaucg agauggacaa gaccaaguac     2940

gaguucugcu ucagccuguu caaggacgac cugaucaagg ugcggaagaa gaagaugaag     3000

gaggccgagc ucugcuacuu cgucggguuc gacaccgacg gcucccgcau caucguggcc     3060

aagcacaaca acgacuacca gaccaugacg gagaacgagu gcaagcuguu cacccugaag     3120

gagaagaaga agaagcagaa gacccucaac gacgaggagg acaaccagga gcggaucgag     3180

accgaguaca agaucaucgg cgggcgcacg ggcauccaga accuggagac cuucgagaag     3240

caccagguga gcccccuggg ggagguccag aaggccgagu ucgaggaccg gcaggagauc     3300

aagcucaagu ccagccccaa gggcaaggac uga                                  3333


<210>  1606
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1606
augagcgaga aguaccgccu gggccucgac guggggacca acucccuggg cugggccguc       60

aucgggcuga aggaggacgu gcccagcucc gugcucgcca ccggcguccg gaucuucagc      120

gaggggcgcg acgcgaagac gaaguccacc cugaaggcca gccggaccga gaagcgcucc      180

gcccggcgcc ggcgcgaccg guuccugcag cgccagaccu uccucaucag cgagauggug      240

aaguccggcc uguucccgaa ggacaucgug gagcagaaga accuggagcu ccuggacccc      300

uacgagaucc gguucaaggc ccugacggag cagguccccc uccaccaccu gggccgcgcc      360

cuguuccaca ucaaccagcg gcgcggguuc aagagcuccc ggaaggacaa gagcgaggag      420

accaccuccg gcguggugag caaguccguc cgcgcgcucu acgaggagau gcagcugauc      480

aaccccgccc cggugaucga cgaccccaag gcccugagca aggaggagcg gcgcgagauc      540

cgggugcagg aggccuccag ccgcgaggag gcgcucguca cccuggccga gcagaagggg      600

gugacguucg gcgccuuccu guaccggcgc cuccaguccg gcaagagcgu gcgcgcccgg      660

aaggacgccg acagccagcu guacgacguc uaccccaccc gcgagcugcu caaggacgag      720

uacuccaagc ugaugcagca ccaggcgcag uacuacccca acgugaucaa cgaggcccug      780

aucagccggc uccacgacgu gaucuucuuc cagcgcaagc ugaaggagca ggagcggggg      840

uucugcaccu acuacccguc cgagcgccgg accuuccgcu gcaugcccag cuuccagcag      900

uaccggaugg uccaggagcu gaacgcccuc gagugggugg acgccgccgg caagcacugc      960

cugcgcgacu accccgaguc ccgggaccug auccucagcg agcuggagaa caucuccacg     1020

aaggcggggg aguucaccuu cggcaacaug aagaagaucc ugaagaagau gggggugauc     1080

gacggcgagg ucagcuucaa cuucgagggc cccaagcgca agggguucca gggcaacgug     1140

accucccggg agaugcgcca cgaggacugc uucgggaccc ggugggacga gcucagccug     1200

gacgagaagg acgacaucau cgacaagauc aacgacgacc accuggacga cgaggacaug     1260

gagcgcuacc ucaucgacac guacggccug uccagcuucu ccguggggaa cgccaucaac     1320

gcccggcugc ucgagggcac cgccaacguc agccgcaagg ccgcgcggac ccuguacgag     1380

accaugcugu ccgccaacug ccuccagagc gacgccguga ucgccgucuc cgagcaggac     1440

gacacguucg ugaacccgua cagccgcacc ggccgcgggg agcugcuguc cgagcucccc     1500

uacuacggcg aggccuucca ggacgggcgg cacaucaucc ccggcaagcg cgagcccaag     1560

gugagccacg accagcugaa guucuacggg ggcgucagca acccgaccgu gcacaucgcg     1620

cugaaccaga uccggcuggu ggucaacgag aucaucaagc gcuacggccu ccccuccagc     1680

aucuccaucg agcuggcccg gaaccugccc gaggggcagg acggccgccg gaagaucgag     1740

aagaugcaga gcgagaacca gaagaacaac gagcgcuacg accagauccu caccgagaac     1800

gggcaguccc ccaaccggga caaccgccug cggcuggccc ucugggagga gcugggcgag     1860

gggccgacgg accgccugug cguguucacc ggcaagcuca ucgagcuggc cgaccuguuc     1920

aacggcagcg uggagaucga gcaccucauc cccuucuccg ucagccugga cgacuccaag     1980

gcgaacaaga ccgugugcug gaaggaggcc aaccgguuca aggggaacaa gacccccuac     2040

gaggccuucg ccaacaacag cggcgacuac uccugggagg agaucaugga gcgcgugcgg     2100

gcccugccca acagcaagca guggcgcuuc caggaggacg cgcucaagau cuggaacucc     2160

gaccacagcg acuucuccgg gcggcaccug aacgacacgc gcuacaucag ccggcuggcc     2220

cgcgaguacc ucgagaacau cugcccguuc aacaagaucg acgucgugac cggccggcug     2280

accgcccugc uccgcggcca cugggggcug aacaacaucc ugcgcggcca caacgagccc     2340

gagaccgcca cgcccaagaa gaugcgggac gaccaccgcc accacgccau cgacgcggug     2400

gucaucgcca ugaccucccu caccauccug cagaagguga gcagcgccgc cgggaaggcc     2460

gaggagcugg acgugggcaa gcuguucgcg aaggacgagu ccgggcggag cgucaucgac     2520

cccugggacg gcuuccgcga cgagguggcc gagaaggugc ggaacaucau cgucucccac     2580

aagaccaagc gcaagacgau cggcaacagc gggaagucca ccgaccgggc ccuccacaac     2640

gagaccgccu acggccugau cgacgggccg gacgagaagg gccucuacag cguggugacc     2700

accaagccca ucuccuacuu cgacaaccgc gacaagaucg agaagauccg ggacgagaag     2760

cugcgcgacg acuuccugac ggcgugggac ggggagggcg acaacaaggc cagccagcuc     2820

gccguccugg acaaggccuc cggccugaac gugaagaagu gccgguacgu ggagcgcaag     2880

aaggucaucc ccaucaacgg gccggacggc cagcccuaca aggccuacga cgggaacagc     2940

aacuggggca uggagaucua cgaguucccc aacgggcacc ccaaggagaa gaaguggaac     3000

ggcgugguga ucuccaccua cgacgcgaac aagagcgagu ucaagccggg cgagaccuuc     3060

cggccccacc ccgccgccaa gcucgucaug cgccugcaca ucaacgacau cguggaggug     3120

cagaacgacg gggagcccau ccuguaccgg guccagcuca ucuccgccgg cacccugacg     3180

gugagcccgc ugcacgaggc caacguggac ucccgcaacc gggacaagca cgacgaguuc     3240

aaguacaaga gccucuccgu cacccccaug cagaagaugg gggcgaagaa ggugaacauc     3300

agccccaccg gccugaagaa cuacucccgc uga                                  3333


<210>  1607
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1607
augggcauca ucaucgggcu ggacaucggc aucgccagcg uggggaucgc cgucguggac       60

aaggacaccc ucgacaucaa gugcgugguc uccgaccugu ucgacagcgc ggacgccucc      120

aagaacaucg agcgccggaa caaccgccag gcccggcgcc ugaaccggcg ccggaagacc      180

cgcaucggcg acuucaacac gcucugggug agcauguucg gggagcugcc cgaggaggug      240

gacuccaacg uccugcuccu gcggaacgcc ggccuggacg agcagcucac ccuggacgag      300

aucuacugcg ugcugaagua caugcucaag caccgcggca ucagcuaccu ggaggacgcc      360

cugaacgagg agaacgugac cggguccuac cagaagggca ucgcgaucaa ccagcgggag      420

agcgaguaca ugcucccgug cgagauccag cuggagcgcu uccggaagua cgggcaguac      480

cgcggcgagu gcgaggccga gaacgagaac ggggagaaga ucacccuguc caacgucuuc      540

augacgaaca gcuaccggaa ggagaucgac aaguuccuca acacccaggg caaguacggc      600

auccugaacc agaaguucau cgacgaguac cugaagaucu ucaaccgcaa gcggaaguac      660

uacgaggggc ccggcaacga gaagucccgc accgacuacg ggaaguacac cacgggcaag      720

gaccucgacg gcaaguacau caccgagaag aacaucuucg agaagcugau cgggaagugc      780

agcguguaca aggaggagcu gcgggccgcc ggcgcguccu acaccgccca ggaguucaac      840

auccucaacg accugaacaa caucaccgug aacaacaaga agcugacgac ccagcagaag      900

aaggacguca ucgagaucgu gcagaacagc gaccgcauca acauggagaa gaucaucucc      960

agcugcaucg gggagaagau cgagaagauc gagggcgccc ggaucgacaa gaacgagaag     1020

aacaucuacc acaccuucga gcaguacaac aagaugcgca agaucuucgc cgagaagaac     1080

uacgacauca aggagcucac caucgagcag cuggacgaga ucgggcgcau ccugacgauc     1140

aacaccgagc gggagggcau caaggacgcc aucauggagu ccgagcuccu gcaccugaac     1200

aacgagcaga ucaaccuccu gaucgagcug cgcuacaaga acagcucccu cuucaccaag     1260

uggcagagcc ugagcaucaa ggcgaugcgg gagaucaucc ccgaccugua cgaccgcaac     1320

gugaaccaga ugaacauccu cgccgagcgg ggcgucuuca aggacaagau caaccuguuc     1380

gagaacuccg uguaccugcc caaggagccg gugcucgagg agaucuacaa ccccgucgug     1440

cgccggagca ucuccaugac caucgacgcc cugaacgccc ugaucaagaa guacggguac     1500

cccgaggaca ucgugaucga gaugccccgc gaccggaacg acgccgagca gaaggaccgc     1560

aucaagaagu uccagaagac gcgggagaac gagcucaaga acaucaagaa gaagaucaag     1620

gaggaguacg gcaucgagau caccgacgag uacuuccgca agcacaaggg gcuggagcug     1680

gagcucaagc ugugggucga gcagaacgag aagugcccgu acagcggcaa guacaucaac     1740

gugcaggaca uccuguacca ccacgagcug uucgagaucg accacaucau cccccucucc     1800

aucagcuacg acgacucccg gaacaacaag gugcuggucu acaaggagga gaaccaggac     1860

aaggggaacc gcacgccccu gauguaccuc agcaccguga accggcagua cgaccagcac     1920

gaguucauga acuacguccu guccaccaag acccugcccc gcaagaagcg ggagcuccug     1980

cugaacuacg aggacaucac caagaucgac gugcucaagg gcuucaucaa ccgcaaccug     2040

aacgacaccc gguacgcgag ccgcuccgug cugaacgcca uccagcuguu cuucggcggg     2100

aaggagugcg acacgaaggu ccgcgugauc cggggcgccg ugaccaacca gcuccgcauc     2160

aagcugaacc ucccgaagga ccgggagaag uccuacagcc accacgccau cgacgccgcg     2220

aucaugugcu acagccagau ggggcugaug aacuuccgca acauccagaa caaguucauc     2280

gacuucgaga acgagaccua ccuguccgac gagcgggaca gcugcuucga caacaaguac     2340

uacgacgagg cccucuucgg ccagguccug aagaugcgca agaaccugcu caaggccgag     2400

gggaagcccc agcgguccgu gcagagcaag ggcguguucg acgaggagga gaagaacaac     2460

ggcaucgucc gcuaccacca caagguggac aagaagccca accgggggcu guccaacgcc     2520

agcaucuacg gcacccgcaa gaucgacgac aagaucuaca agaucguggg guacgacauc     2580

cggaacaaca aggacgccga ggcgaucauc aacaucauca agaagggcaa gagcgacaac     2640

uuccugaugg ccaagcacga ccccgagacg uucaagaacc ucauggagau cuacgagcag     2700

uacaaggagg agaagaaccc guuccuggcc uacgagcagg agaccggcga cuacauccgc     2760

aaguacgcca agaaccacaa cgggcccauc aucaagaaga ucaaguacgu cgacuccgag     2820

gugggcagcu gcaucgacau cuccgcgaac uacggguaca agaacggcga caagcgggug     2880

aucauggaca cccugacccc cuaccgcgcc gacgucuacu acaacuacaa ggacgggacg     2940

uaccggauca ucggcgugaa guacgccgac uucaaguaca gcaacgccaa guacgugcuc     3000

gacgaggagg ccuacgcgcu gauccugauc aaggagggcc uccugaagau cggggacucc     3060

cuggagaaca ucagcuccag cggcuacgag uaccgccucu ccuucuacaa gaacgacauc     3120

aucgaguaca ccaagaacga caucgucuac aaggagcggu uccugagccg caccaugccc     3180

aaggugaaga acuacaucga gaccaagccg aucuccgccg cccuguacga gaagcggaac     3240

cccaucgggc ucaccaacgc cacgaacauc ugcaagaucu acaccgacgu gcugggcaac     3300

gagaccaaga ucuacaagga gaaguucaag cugagcgucg acuga                     3345


<210>  1608
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1608
augaacuaca ccgagaagga gaagcuguuc augaaguaca uccucgcccu ggacaucggc       60

aucgccagcg uggggugggc gauccuggac aaggaguccg agaccgucau cgaggccggc      120

agcaacaucu uccccgaggc cuccgccgcc gacaaccagc uccgccggga caugcgcggg      180

gcgaagcgga acaaccgccg gcugaagacg cgcaucaacg acuucaucaa gcugugggag      240

aacaacaacc ucagcauccc gcaguucaag uccaccgaga ucgugggccu gaaggugcgg      300

gccaucaccg aggagaucac ccuggacgag cucuaccuga uccuguacag cuaccucaag      360

caccgcggga ucuccuaccu ggaggacgcc cuggacgaca cggucagcgg cuccagcgcc      420

uacgcgaacg gccucaagcu gaacgccaag gagcuggaga cccacuaccc cugcgagauc      480

cagcaggagc ggcucaacac caucgggaag uaccgcggcc agucccagau caucaacgag      540

aacggggagg ugcuggaccu gagcaacgug uucaccaucg gcgccuaccg gaaggagauc      600

cagcgcgucu ucgagaucca gaagaaguac caccccgagc ucacggacga guucugcgac      660

ggguacaugc ugaucuucaa ccggaagcgc aaguacuacg agggccccgg caacgagaag      720

ucccggaccg acuacgggcg cuucaccacc aagcuggacg ccaacggcaa cuacaucacg      780

gaggacaaca ucuucgagaa gcucaucggg aagugcagcg uguacccgga cgagcugcgg      840

gccgcggccg ccuccuacac cgcccaggag uacaacgugc ugaacgaccu caacaaccug      900

accaucaacg gccgcaagcu ggaggagaac gagaagcacg agaucgucga gcggaucaag      960

agcuccaaca ccaucaacau gcgcaagauc aucagcgacu gcaugggcga gaacaucgac     1020

gacuucgccg gggcgcggau cgacaagucc ggcaaggaga ucuuccacaa guucgaggug     1080

uacaacaaga ugcgcaaggc ccuccuggag aucgggaucg acaucagcaa cuacagccgc     1140

gaggagcugg acgagaucgg cuacaucaug acgaucaaca ccgacaagga ggccaugaug     1200

gaggccuucc agaaguccug gaucgaccuc agcgacgacg ugaagcagug ccugaucaac     1260

augcggaaga ccaacggggc ccuguucaac aaguggcagu ccuucagccu caagaucaug     1320

aacgagcuga uccccgagau guacgcgcag cccaaggagc agaugacccu gcucacggag     1380

augggcguca ccaagggcac ccaggaggag uucgccgggc ugaaguacau ccccguggac     1440

guggucuccg aggacaucuu caacccggug gugcgccgga gcguccgcau cuccuucaag     1500

auccugaacg ccgugcucaa gaaguacaag gcccuggaca ccaucgugau cgagaugccc     1560

cgggaccgca acagcgagga gcagaagaag cggaucaacg acucccagaa gcugaacgag     1620

aaggagaugg aguacaucga gaagaagcuc gccgucacgu acggcaucaa gcugagcccc     1680

uccgacuuca gcucccagaa gcagcugagc cucaagcuga agcuguggaa cgagcaggac     1740

gggaucugcc uguacuccgg caagaccauc gaccccaacg acaucaucaa caacccgcag     1800

cuguucgaga ucgaccacau caucccccgc agcaucuccu ucgacgacgc gcggagcaac     1860

aaggugcucg uguaccgcuc cgagaaccag aagaagggga accagacccc cuacuacuac     1920

cugacccaca gccacagcga gugguccuuc gagcaguaca aggccacggu caugaaccuc     1980

agcaagaaga aggaguacgc caucucccgg aagaagaucc agaaccugcu guacagcgag     2040

gacaucacca agauggacgu gcucaagggc uucaucaacc gcaacaucaa cgacaccucc     2100

uacgccagcc ggcugguccu gaacaccauc cagaacuucu ucauggcgaa cgaggccgac     2160

acgaagguga aggugaucaa gggcuccuac acccaccaga ugcgcugcaa ccucaagcug     2220

gacaagaacc gggacgagag cuacucccac cacgccgucg acgccaugcu gaucggguac     2280

agcgagcucg gcuacgaggc cuaccacaag cugcaggggg aguucaucga cuucgagacc     2340

ggcgagaucc ugcgcaagga caugugggac gagaacaugu ccgacgaggu guacgcggac     2400

uaccucuacg ggaagaagug ggccaacauc cggaacgagg uggucaaggc cgagaagaac     2460

gugaaguacu ggcacuacgu gaugcgcaag agcaaccggg gccugugcaa ccagaccauc     2520

cgcggcacgc gcgaguacga cgggaagcag uacaagauca acaagcugga cauccggacc     2580

aaggagggca ucaaggucuu cgccaagcuc gccuucucca agaaggacag cgaccgcgag     2640

cggcugcugg uguaccucaa cgaccgccgg accuucgacg accugugcaa gaucuacgag     2700

gacuacuccg acgcggccaa ccccuucgug caguacgaga aggagaccgg ggacaucauc     2760

cgcaaguaca gcaagaagca caacggcccg cggaucgaca agcugaagua caaggacggc     2820

gaggucgggg ccugcaucga caucucccac aaguacggcu ucgagaaggg gagcaagaag     2880

gugauccucg agagccuggu gcccuaccgc auggacgucu acuacaagga ggagaaccac     2940

uccuacuacc uggugggcgu gaagcagagc gacaucaagu ucgagaaggg gcggaacguc     3000

aucgacgagg aggccuacgc ccgcauccuc gugaacgaga agaugaucca gcccggccag     3060

ucccgggcgg accuggagaa ccugggcuuc aaguucaagc ucagcuucua caagaacgac     3120

aucaucgagu acgagaagga cgggaagauc uacaccgagc gccugguguc ccggacgaug     3180

cccaagcagc gcaacuacau cgagaccaag ccgaucgaca aggccaaguu cgagaagcag     3240

aaccuggucg gccucgggaa gaccaaguuc aucaagaagu accgguacga cauccugggc     3300

aacaaguaca gcugcuccga ggagaaguuc accagcuucu gcuga                     3345


<210>  1609
<211>  3348
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1609
augagcgcca ccuacaccau cggccuggac aucgggaucg ccuccguggg cuggugcgcg       60

uucggggaga cgcacauccu cgaccugggc guccgcgccu ucgacaaggc cgagaccgcc      120

aaggaggggg agagccugaa ccucgcccgg cgcaacgcgc ggcugaugcg ccggcgccug      180

uuccggcgca ccuggcggcu caagaagcug gcccgccagc ugaaguccga gggccucauc      240

gccgacacca agcuguucaa gcccgagcug cccuucagca agucccucug gcagcugcgg      300

guggacggcc uggaccgccg gcucaacgac gaggaguggg cccgcgugau cuaccaccug      360

ugcaagcacc ggggguucca cuggaucagc cgcgcggagg ccaagcaggc cgagggcgac      420

uccaagagcg agggcgggaa ggucaagcag gggcuggccg gcacggccaa gcucauggac      480

gagaagggcu accgguccgc ggccgagaug gugcuggccg aguuccccca ggcccagcgc      540

aacaagcagg gggaguacca gaaggcccug agccgggagc uccuggcgaa ggagcuggcc      600

cuccuguucg cccagcagcg cgaguacggc aaccugcaca cccccggggc ccucgagacc      660

gccauccugg gcaacggcga caagaagucc gggcuguucu ggcagcagaa gcccgcgcuc      720

gccggcaccg accugcugaa gaugcucggg acgugcaccu ucgagaaggg cgaguaccgg      780

gccccgaagg ccagcuucac cgccgagcgc cacguguggc ugacccgccu gaacaaccuc      840

cggaucguca cggacggggu gacccgccag cugaccgagc aggagcggca gcugugccuc      900

ccccugcccu accgccaggc gggcgaccug accuacaagc agcucggcuc cgcccuggcc      960

aaggccgggc ugcucgaggc gggcggguuc aaguucacgg gccuggccua ccccaccgag     1020

gcccagaagg ccgaggggaa ggccaaggac ccggagagcg cgacccuggu gaagaucccc     1080

gccuggcacg agcuccggca gacccugacg aagggcggcc ugaacgacga gugggagaag     1140

cuggccgggg acgcccucug cggccagccc gagaucuucg acaagaucgc cuggguccug     1200

uccguguaca aggacgacga cgaggugcgc gcggagcugg ccaagcucgc ccugcccaac     1260

ccgcaggccc ugaccgaggc ccuccugaac auccgguucg acaaguucca cagccugucc     1320

cucaaggcgc ugcgcgccau cgucccccac auggagcagg ggcugcggua cgacgaggcc     1380

ugcgugcagg ccggcuacca ccacagccag cuccugaagg ugggggaggg cgagcacaag     1440

uaccugccgc cccucuacga cggccgcgac ggggacggcc ggaugaucuu cgcggaggac     1500

gccgacaucc cccgcaaccc cgucgugcug cggucccuga accaggcccg caaggugguc     1560

aacgccauca uccggcgcua cgggagcccc agcgccgugc acaucgagau ggcgcgggac     1620

cucucccgcc ccuucgacga gcggaacaag aucaagaagg accaggacga guaccaggcc     1680

cgcaaccagc gcgaccggga gcaguucagc gagcuguucg gccacccgcc caagggccug     1740

gaguucgaga aguggcgccu cuaccgggag cagaacgggc agugcgccua cucccagcgc     1800

ccccugaccg ccagcggcaa cguggccgag aucuuccugg acggguccac cgagaucgac     1860

cacgcgcucc ccuacagccg guccuucgac gacagcaaga acaacaaggu ccuggugcug     1920

acgcgcgaga accgggacaa gggcaaccgc accccguacg aguaccucgg gggcgccgag     1980

aacuccgaga gcuggcagcg guucgugucc uucgucgaga gcaacaaggc cuaccgccug     2040

gccaagcgga cccgccugcu ccggaaggac uucggcgcca aggaguccga ggaguuccgc     2100

gagcggaacc ugaacgacac ccgcuacgug ugccgguucu ucaagaacua cgucgagcag     2160

caccugaagc ucgcggaggg gagcgagacg aagcgcugcg ugguggucuc cggccagcug     2220

accaacuucc ugcgggcccg cugggggcuc cugaaggugc ggagcgacuc cgaccgccac     2280

cacgcccugg acgccgcggu ggucgccgcc ugcagccacu ccauggugca gcgccucagc     2340

aacuacagcc ggacccgcga gcuggagcag guccgggacg gcuucgugga caucgagacc     2400

ggggagaucg ugaaccccgc caugcaccag cagcugcgcg agcacuuccc cgaccccugg     2460

ccguacuucc ggcacgagcu cgaggcccgc cugaagaucg acucccccga gcugcuccgg     2520

gaggagaugg cgcgccuggg cagcuacucc gaggaggagc ugaaggugcu caagccccug     2580

uucgucagcc gggccccgca gcgccggaac ggcggggccg cccacaagga cacgaucuac     2640

ggccagcccg agaagcugaa gaagaacggg uccgugaccc agaagguggc ccucaccagc     2700

cugacccuga aggacaucga ccuccuggcg gacccccacc gcaacgccaa gcuguacggc     2760

gccauccggg agcgccucga ggcccacggg ggcaagggcg acaaggccuu ccccgcgacg     2820

aacccgcugc ggaagcccga caaggagggg aaccccaccg gccccaucgu ccgcaccgug     2880

accaagguga ucgacaagcu guccgggauc ccgguccggg gcgggaucgc caagaacgac     2940

accaugcucc gcguggacgu guucacgaag ggcggcaagu uccaccuggu ccccguguac     3000

gugcaccacc gggucaccgg gcugcccaac cgcgccauca ucgccuucaa ggacgaggac     3060

gaguggaccc ucaucgacga cagcuucgac uggugcuucu cccuguaccc caacgaccug     3120

cuccgcauca agcugaagaa ggagagccac uccggcuacu acagcuccug caaccggagc     3180

accggggccg ugaaccugug ggcgcacgac cgcaacguga acgucggcaa ggacggcuuc     3240

aucgagucca ucggggugaa gauggccgug agcaucgaga agcuccacgu cgacacgcug     3300

ggcaacaucu acccggcccc gcccgagaag cggcgcgggc uggccuga                  3348


<210>  1610
<211>  3351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1610
augagccaga accagaaccc caacacccag ucccaccuga acccgaccac gcagagcucc       60

acccagagca acccccagcc caccacccag gagucccuca agauccuggg cuucgacauc      120

gggguggcca gcaucggcug ggccuucgug gagggcgggg agcugaagga cugcggggug      180

cgcaucuuca cgaaggcgga gaaccccaag gacggcucca gccucgcccu gccgcggcgc      240

gaggcccggg gcgugcgccg gcgccuggcc cggcgcaagg ggcggcucaa cgcccugaag      300

guccugaucu gcaaggaguu cggccucaac cgcuccgacu accuggcgac cgccggggag      360

cugcccaagg ccuacgccac cagcaaggag accaaguccc ccuaccagcu ccgggcggag      420

agccuggagc gcaagcuguc cgccgacgag uucgcccggg ugauccucca caucgccaag      480

caccgcggcu acgggaacaa gcacgccaag gagagcuccg acgacgacag cggcaaggug      540

aagaaggcga ucuccgagaa ccagaagguc augagcgaga agggcuacac gaccgccggg      600

caguaccugu acggcgaguu cuaccagaag gcccggaacu ucuccgaggc cgagcccaag      660

agcccgaaga acgcccgcgg gacccaggag uucaagaacg ugcggaacaa gagcgagaac      720

uacgcgcgcu gccuggccca guccgagcuc cagaaggagc uggagaucau cuucgccaag      780

cagcgggagu ucggcuucca guucagcgac aagaagcaca agaccaucaa cgccaacggc      840

aagcugaagg agcucgacuu cgccgacgcg gugcuggaga ucgccuuccu gcagcgcccc      900

cucaaguccu ucgccgacaa ggucgggaag ugcacguucu acgaggacaa gccccgggcc      960

cccaaggaca gccuguccgc caucgaguuc guggcgcuga cccgcaucau caacacccuc     1020

gccaacauca ccaagcgcag cggcgaggug uacuccgccg acaagguccg ggagauccug     1080

agcaucgugc uggacaaggg ggagaucacg uacaagaacc uccgcaaggu gaucgcccug     1140

gacgagcggc ugcaguuccc gaaggacucc aagcucgacu acgcgaagga ggacgccgag     1200

aaggccaagg ccaucgaguu cagcaagcug aaggccuucc gcaaggcgcu gggcgggucc     1260

uucgagggcu ucauccgggc cgagcucgac agcaucgcca ccgacaucgc ccugaucaag     1320

uccaaggacg agcugaaggc caagcucgcg aacuaccaga gccugaacga cgagcagcgc     1380

gagaagcugu ccgagcucag cuucgacaag uucaucgacc uguccuucaa ggcccuggag     1440

gccauccucc cccugaugcg gggcgacaag gacgacaaga ucuaccgcua cgacgaggcc     1500

uggcgggagg ccgggcuggu cgagguggcg aagaagaccc agaagggcga cagccucccg     1560

ccccugaucg aguacgagaa ggaccuggcc aaccccgugg ucgcccgcgc ccuggcggag     1620

uaccggaagg ugcucaacgc ccugcugaag aagcacggga aggugcacaa gauccaccuc     1680

gaguucaccc gccaggccgg cgucuccagc aaggagcgcg gggagaucau cgccgagcag     1740

aacaagcggc ugaaggccaa cgaggaggcg cggaagcgcu gcgaggagua cggccugauc     1800

cccagcggca agaacauccu caagcugaag cuguggcagg agcaggggga guucugcgug     1860

uacuccggca agaagaucac gcagagcgac cuccgggacg aguccacccu gcagaucgac     1920

cacaucuacc ccuacagccg cuccuacgac gacagccaga acaacaaggu gcuggucuuc     1980

accgacgcca accagaacaa gcucaaccag acccccuacg aggccuucgg gaaggacauc     2040

uccaagugga accagauccu gggccggauc gagaagcugc cgaaggccaa gaagaaccgc     2100

aucacgaaca agaacuucgc caacaaggag gcgggguuca ucgcccgcaa caucguggac     2160

accagcuaca ucgcccggcu cuccgccagc uacaccgacg ccuucaucga guucugcccc     2220

cuggcggaga acgaggacac cacgcugggc cgcggcgaga agggguccaa gaagcacauc     2280

agcgucguga acggcucccu caccgccacc augcggcacu acugggggcu gaacacccug     2340

cucgacggcg agaacagcga guccaaggac cgcagcaacc accuccacca cgcccuggac     2400

gccaucauca ucgcguacgu gaacgacucc gucaucaagg ccuucagcga cuucaagaag     2460

acgcaggagc agaacaaggc ccggcugauc gccaaggaga ucgagagcgc cgaguacaag     2520

aucucccgcc gguucuucag ccccuccggc uucgagaaca acgaggcguu ccgccaggcc     2580

aucaagcgga agauccuggg gcagagccug gagucccgcg agaacgacau gaccaucaac     2640

agcgaggccc ucggcgagaa ggucgggggc aucuucgugu ccaagccccc gcggaagcgc     2700

gcccgcgggg cccugcacaa ggagaccuuc agcuccaucg acgacaagga ccugcucaag     2760

accuacggcg gcaaggaggg ggugaaccgg gcgaucaagc ugggcaaggu gcggcagauc     2820

gggagcaaga ucgccgacaa cggcacgaug gugcgcgucg acaucuuccg gcacaaggug     2880

uccgggaagu ucuacggcgu gcccaucuac accauggacu ucgcccuggg cauccucccc     2940

aacaaggccg ucgucggggg caaggacaag agcgggguga ucaaggacug gcugcccaug     3000

gacuccaacu acgaguucug cuucagccug uucaaggacg accucauccu ggugcagaag     3060

aaggagaugu ccaaggccga gcugugcuac uucgcgggcu ucgacaccag cgggggccag     3120

aucaaggugg agaagcacga caaccacuuc gccacccuca ccgacaacca gcgccugcug     3180

uucaacaucg ccacgccgaa ggagguggcc ggcaagucca ucgggaucca gaaccucaag     3240

gucuucgaga aguggcaggu cagcccccug ggcgaggugu ccaaggccga guucaaggag     3300

cgggagccca ucgcgcucaa gagcaccccg aagcgcuucc ugcaggggug a              3351


<210>  1611
<211>  3354
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1611
augaagcaca uccugggccu cgaccugggg agcaacucca ucggcugggc cuucgugcag       60

caggacuucg agaacaagca ggggaagauc aucgccaccg gcagccgcau cauccccaug      120

gaccagggga uccugggcga cuucgagcgg ggcaacaccg ucucccagac ggcggagcgc      180

accaccuacc ggagcaugcg ccggcuccgc gagcggcacc uccugcgccg ggagcgccuc      240

caccgggugc ugcaccuccu cggguuccug ccgccccacu acgacgccca gaucgacuuc      300

accaagcgcu acggcaaguu caucgacaac gccgagccca agaucgccua caacaacggg      360

aacuucaucu ucaugaacuc cuucaacgag augguggagg acuucaagaa gcaccagccc      420

cagcuguucu acaagaaguc caacggcgag gagagccgga ucccguacga cuggacgauc      480

uacuaccucc gcaagaaggc ccugagccag aagaucaccc agcaggagcu ggcguggcuc      540

auccugcacu ucaaccagaa gcggggguac uaccagcugc gcggcgagga ggagaccgag      600

aaccccaaca aggaggucgc cuuccacucc cucaaggugg uggacgucga ggccgaggcc      660

cccaacaaga agggcgagau cugguacacc auccggcugg agaacgggug gaucuaccgc      720

cggacgagca agaacccccu ggacgacugg aagggcaaga cccgcgacuu caucgugacc      780

accgaccuca acgacgacgg gacgaucaag cuggacaagg agggcaagga gaagcggucc      840

uuccgcgcgc cggacgagaa cgacuggaac cugcucaaga agaagaccga gcacgacauc      900

gacuucagcg gcaagcccgu ggggaccuac aucuacgacc accuccugca gaaccccaac      960

cagaagauca agggcaagcu gguccgcacc aucgagcgga aguacuacaa ggacgagcug     1020

aaggccaucc uggccaagca gaucgcccuc cagcccgagc uguucacgga gacccugcuc     1080

gccgacugca uccgcgagcu guaccggaag aacgagaccc agcagcagaa ccugcucucc     1140

aaggacuucc ugcaccuguu cguggaggac aucaucuucu accagcgccc gcucugcagc     1200

aagaagucca ccaucgcgaa cugcacgcug gagaagcgga gcuacaucga caaggagacc     1260

aacgagcgca aggacgcccc caucaaggug ugcgccaagu ccaaccccua cuaccaggag     1320

uuccgggucc ugcaguggcu ccagaaccug cgcaucuacg agaucgagac cgaccgggag     1380

gugaccgccg aguucaucga gacgccccag gacuacgagg cccuguucaa cuuccucaug     1440

agccagaagg agaucgacaa cgaggggcug cugaaguacu uccucuucgc gaagaacccg     1500

cagaucaagg acaaggccgc caaguccgag cugaagaagu ggcugaccac cuaccgcugg     1560

aacuacgugu acgacgccgc cgacaacagc agcaagaagu accccaugaa cgagaccggc     1620

uacgagcugc agcgguaccu ccagaagguc gaggggcugc ccgcgaacuu ccugacguuc     1680

gacaucaccu accagcucug gcaccuccug uacuccguga aggacaaggu ggaguucgag     1740

aaggcccuga agaaguucgc caagaagaag cagcuggaca agaccagcuu cgucgacucc     1800

uucaagaacu ucaagcccua cccgaacgag uacggcagcu ucuccgagaa ggccaucaag     1860

aagcugcucc cccugaugcg cuucggcaac cacugggacu ucaaccggau cgacgugagc     1920

acccagaagc gcaucgacga ccugaucaac gggguggcca acgaggacau ccggacgauc     1980

auccgcgaga aggcggagaa guaccagcuc aacuccgaga ccugcuucca ggaccugccc     2040

cuguggcucg cccaguacgu cguguacaac cggcacgccg aggccaccga caucgagaag     2100

uggaccagcg uguccgaccu ggaggcguac cuggaggacu ucaagcagca cagccuccgc     2160

aaccccaucg ucgagcaggu cgugacggag acccugcggg uggugcgcga caucuggcag     2220

caguacuccg gcgggcaggc caacuucuuc gacgagaucc acaucgagcu gggccgcgag     2280

cucaagaaga ccgccgagga gcggaagcag cugagcgaca agaaccagga gaacgagaac     2340

accaaccugc gcaucaagaa gcuccuggcc gagcugaagg aggacgccuc cauccagaac     2400

guccggccgu acagccucgu gcagcaggag cugcugaaga ucuacgagga ggggguguuc     2460

uccagcaccg acgagaucga ggaggacauc cagaagaucc gcaagaaggu cgagcccacg     2520

ccgcccgaga uccagcggua caagcucugg cuggagcaga aguacaaguc ccccuacacc     2580

ggcgagguga ucagccugac caagcuguuc accgaggagu acgagaucga gcacaucauc     2640

ccccaguccc gcuacuacga cgacagccug agcaacaagg ugaucugcga guccgcgguc     2700

aacaagcuga agacgaacca gcucggccug gaguucaucc agaaccacca cggggagaag     2760

gugcggauca ccgccaacaa ggaggugacc aucuucaccg aggagcagua caaggaguuc     2820

gucaagaagc acuacgccaa caacagcgcc aagcagcaga agcugcuccu gaacgagauc     2880

cccgagaaga ugguggcccg ccagcugaac gacacgcggu acaucuccaa gugcaucagc     2940

gagauccucu ccaagaucgu gcgcagcgac gagaaggacg agggcgucaa cuccaagaac     3000

aucaucccgu gcaccgggaa gaucaccacc gcgcugaagc aggacugggg ccugaacgac     3060

guguggaacg agcucauccu gccccgguuc gagcgcauga accagcugac gcagacccag     3120

cuguucacca gcuacaacga ccggcugcag aagaaccugc ccaccgugcc caucgaguac     3180

ucccaggggu uccagaagaa gcgcaucgac caccggcacc acgccaugga cgcccucguc     3240

aucgccugcg ccacgcgcga gcacaucaac uacaugaaca accagcacgc gcuggacaag     3300

agcaaggaca agaaggagaa gcagcagucc cggaucgacc ugcacccgcu cuga           3354


<210>  1612
<211>  3354
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1612
augcagacca agaaccugcu cuacacccug ggccuggaca ucgggaucgc cagcgugggc       60

uggugcgucc ucggggagaa ccgcaucauc gaccugggcg ugcggugcuu cgacaaggcc      120

gagacgcccg accgcgggga gucccugaac aaggugcggc gcgacgcgcg gcucaugcgc      180

cggcgccugc ggcgccgggc cuggcgccug accaagcucg cccggcugcu gaagcacgcc      240

ggccucaucg acagcacccg caucguccgg gaccgccacg acggcgccgu gucccccugg      300

cagcugcggg uggaggggcu ggaccgcaag cucgcgcccg aggagugggc ccgggucauc      360

uaccacaucg ugaagcaccg cggcuuccac uggaucagcc gggccgagga gcgcaaggcc      420

gaguccgaca agggggaggg cgggaaggug aagcagggcc ugagcgcgac caagcagcug      480

auggucgaga agaacuaccg cuccgccgcc gagauggugc ucacggaguu ccccggcgcc      540

cagcggaaca agcgcgggga guacaccaag gcccugagcc ggauccugcu cgcggccgag      600

cugaccaccc uguucgagcg ccagcaggag cucggcaacc cccacgccgg gccggaccug      660

cagacgaaga uccugggcaa cggcgaccgg aaguccgggc uccuguggau gcagaagccg      720

ccccugagcg gcuccgcccu ccugaagaug cuggggaagu gcaccuucga gcgcgaggag      780

uaccgggccc ccaaggcgag cuucaccgcc gagcgccacg uguggcucac caagcugaac      840

aaccugcggg uccucgugga cggcgugacg cgcccccugu ccgaccccga gcggcgccug      900

gcccucagcc ugcccuacca gcaggccucc gacuucaccu acaagcagcu gcgggccgcg      960

cucugcaaga acgggcugcu gcccgacagc uuccaguucu ccggccucgc cuacccgacc     1020

ggcgcccagc agcccgacgg gaaggccaag aaccccgaga acgaccgccu ggucaagcug     1080

cccgccuggc aggaguuccg gaagacccuc cgcgacgcgg gccuggagac ggagugggag     1140

agcaucgccg ggauggccgc cggcgggcag ccggaccugc uggaccagau cggcugggug     1200

cucuccgugu ucaaggacgg cgacgagguc cugcgggagc ugcgcaagau cccccucccc     1260

gggggcgaga agauggugca ggcgcugagc ggggugagcu ucgacaccuu ccacaaccug     1320

ucccucaagg cccugcggaa gaucgucccc cacauggagg ccggccugcg cuacgacgag     1380

gccugccucg ccgcggggua ccaccacagc gaccugcgca aggacaccga cgagcggucc     1440

cuguaccucc cgccccugua cagcggccgc accacggacg gccggauggu guuccgcgac     1500

gacaucgacg ugccccggaa ccccgucgug cugcgcgcca ucaaccaggc ccggaccguc     1560

gugaacuccg ugguccgccg guacgggagc ccguccagca ugcacaucga gauggcccgc     1620

gaccucuccc ggcccuggga cgagcgcaac aaggugaagc gggcccagga ggaguaccgc     1680

gaccggaacg agaagcugaa gaucgacuuc gcggccgagu acggcaucga gcccaagagc     1740

cgcgaguucg agaaguuccu gcucuaccgg gagcagggcg ggaagugcgc cuacucccug     1800

aagccccugg accucgaccg cgugcugcgg gaccugggcu acgccgaggu cgaccacgcc     1860

cucccguaca gccgcuccuu cgacgacagc aagaacaaca aggugcuggu gcuggcggag     1920

gagaaccgga acaaggggaa ccgcaccccc uacgaguacc ucgacggcac cuccgagagc     1980

ccccgcuggc gggaguucaa ggccuucguc gagacgaaca aggccuaccg ccuggccaag     2040

cgggcgcgcc ugcuccggaa ggacuucucc gggaagggca gcgaggaguu ccgcgaccgg     2100

aaccugaacg acacccgcua caucucccgg uucuucaaga acuacgugga gcgcuuccug     2160

aagcucgccg aggagagcga ggagcggacc ugcguggucc ugagcgggca gcugaccgcc     2220

uuccuccgcg cccggugggg ccuggccaag gugcgcggcg acuccgaccg gcaccacgcg     2280

cuggacgccg ccgugaucgc cgccugcagc cgcuccaugg ucaagcggcu cagcgacuac     2340

gcgcgccggc acgagcugga gaaggugcgc acgggguucg uggacuucga gaccggcgag     2400

aucgucgacg ccgggaagua ccgccagauc gagcgggacu ucccgucccc cuggcccuac     2460

uuccgcgacg agcugcucac ccggcugaac accgacgacc ccgccgagcu gcgcgagcgg     2520

augcagagcc ucggcuccua cccgcccgag gcccuggaga cgcugcgccc cauguucgug     2580

agccgggcgc cccagcgccg gaacgggggc gccgcccaca aggacaccau cuaccgccag     2640

accgagcggc ucaagaaggu gggcuccguc acccagaagg ugccgcuggc cgcccugaac     2700

cuccgcgacc uggagaaccu gguggacccc caccggaacg agaagcucua cgcggccauc     2760

cgcgcccggc uggaggccca cgggggcaag ggggagaagg ccuucccgcc caacaacccc     2820

cugcgcaagc ccgaccggga cggcaacccc acggggccca ucguccgcac cgugacccuc     2880

gugaucgaca agaugagcgg caucccgguc cggggcgggg uggcgaagaa cgacaccaug     2940

cugcgcgugg acaucuuccg guccaagaag gacggcaagu uccaccuggu cccccucuac     3000

gugcaccacg ccguggccaa gcagcugccc acccgcgccg ucaucgccua caaggacgag     3060

ggggagugga cgcccaucaa caaccacgac uucgacuucc uguucgcgcu cuacccgaac     3120

gaccuggugc gcaucacccu gaagaaggag cggcacuucg gcuacuacgc cgggugcaac     3180

cgcgccaccg gcgccgugag caucugggcc cacgaccgga accagcgcau cggcaaggac     3240

ggguucaucg acggcaucgg ggucaagacc gcgcucuccg uggagaaguu ccacguggac     3300

acgcugggca acgucuaccc cgccccgccc gagacccggc gcggccuggc cuga           3354


<210>  1613
<211>  3357
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1613
augggcaccc gcuacagcuu cgacaucggg accaacagca ucggcugggc cguguggcgg       60

acggggcgcg accccaaggg caucuucggg gagaaggacg ccccgcugga gcuccugggc      120

gcgggcgucc ggcuguucaa ggacgggcgc aaccccaagg acggccagag ccucgccacc      180

augcggcgca ucccccggca ggcccgcaag cggcgcgacc gguucgugcu gcgccgggag      240

gcccuggugg ccgcgcucau cgaggccggg cugaugcccg ccuccgagaa ggagcgcaag      300

gcccuggcgg accucgaccc guaccagcug cgggccgccg gccuggacaa ggcccucgcg      360

ccccacgagc uggggcgcgc caucuuccac cuccaccagc ggcgcggcuu caagagcaac      420

cggaagaccg accgcaaguc cgacgaccgg gacaagggca agaucgccac cgccagcgcc      480

gcccucgcga agaaguucga ggacgagaac gcccgcacgu ucggggaguu ccuguggcgc      540

cggcaccgcg accccgacau gaagcggcag gccguccuga aggcccacga ccccgccaag      600

ggcaagccgg accuccgcgc ggccaagcug cggcagccca cccgcauccg gcuggagggg      660

cagggcgcca ccgcccucua cgaguucuac cccacccgcg agaugcugug ccacgaguuc      720

gacgcccugu gggaggcgca ggcccggcac caccccaugc uccugacgcg cgaggccggc      780

gacaagaucc gguccauccu guuccgccag cgggagcuca agccgccccu gaucgggcug      840

ugcaccuucg aguacgccgc gggcgagaag gagagccgcc uccccaaggc ccugcccucc      900

gugcaggccc gggagaucua cgagcgccug gggcacaucc ggcucagcac cggcuccggg      960

accgaccgcc cgcucaagag cuccgagcgg gacgaccugg ccacggcccu gcugcacugc     1020

ggccugacca agaucaaggu gagcaagacc aagggccucg cguucaagac cgaguuccgc     1080

aaggcccugc gccucggggg cgagguccgg aucaacuucg aggagauggg ggaggagggc     1140

cugguggacc ugcgcacgaa ccggcugcuc gugcaggacg accacuacgg gccccgcugg     1200

cgggcccugu ccuggagcgg caaggacgcc uucgucaaga agcugcucga ggccaccgac     1260

gacgacaagc ugguggcgcg ccugaugacc gaggacggcc uguccgccgu gcaggcccgg     1320

aacgccgcca gcaucccccu ccccgacggg uacucccgcc ugggcccgac cgcgaacgcc     1380

gccauccugc aggcccucau cgacagccgg gacgagaacg gguucgucgu gacguacgac     1440

aaggcggugc gccgggccgg cgagcgccug gggaaggacu ggcaccacag cgacgagcgg     1500

gacggcgaga uccuggacgc ccuccccuac uacggcaagg uccugcagcg ccacauccug     1560

cccgggauga uggaccccgc cgaccggaag gacgaggcca aguucuacgg ccgcaucaug     1620

aacccguccg ugcacaucgg gcucaaccag cugcggcgcc uggugaacgc gcucaucgag     1680

cgguucggcc cgcccgacca gaucgucgug gagcuggccc gcgaccugaa gaugagcgcc     1740

cgggagaagg agcaggagca gcgccgcaac cgggacaacc gcgaggccaa cgagaagcgg     1800

accaagaagc ucguggagcu gggccaggac aacaacgggg agaaccgcgc ccggcugaag     1860

cucuacgacg agcagcagcg cgcgggcaac ggggucgccc acugccccuu cacccuggac     1920

cccaucggca ucacccagcu guucuccccc gagaucgaga ucgagcacgu gcucccccgg     1980

agccgcacgc uggacgacuc cgccgccaac aaggugcugu gcuuccggac caucaaccgc     2040

aucaagcggg ggaagacccc gugggaggcc uuccacgcgg accccaagug gcccgacauc     2100

gccgccaacg ccgagaagcu gcccccgaac aagcgcuggc gguuccgcga ggacgcgaug     2160

gacgucuucg aggcccggac cagcggcaag auggacaaga cgacccugga gcagaugggc     2220

cugaccaccg gguuccaggc ccgccaccuc aacgagacga agcacauguc ccggcuggcc     2280

aaggccuacc ugggcaagau cugcgacccc gacgcggugu acgucacccc cggggcccuc     2340

accagccugc ugcgcggcaa gugggggcuc aacuccaucc ugagcgacga caaccugaag     2400

gaccggaccg accaccgcca ccacgccauc gacgccauca cgaucggcgc caugacccgc     2460

ucccuccugc uggagcucaa cacccuggcg ggcaaggccg agcagagcga cuacgacgac     2520

guggugggga agguccccug gccguucgac aacuuccggg acgccgugcg cuccgugguc     2580

gaccgggugg uggucagcaa caagcccgag cacggcaagc agggggcccu gcacgaggac     2640

accgccuacg gcaucguggg ggacgaggcg gaggccgagg agaucggcaa ccucgugcgc     2700

cggaagcugc uggucgaccu cacccccggc gagguggacc gcauccggga cgccgggcug     2760

cgcaaggcca ugcaggccgc gguggccccc ucccgggacg cccgcggcaa ggucagcgac     2820

gccaaggggu ucgcggccgc ccuggacacg uucgccaagg cccagggcau gcggcgcauc     2880

cgggugggga aggaggacau guccgcggug accauccacg accgccagac cgacaaggcc     2940

uacaaggccg ucgccccggg cgagaaccac cacguggacg ugguccagau gcgggacggc     3000

gccuggaagg gguucgcggu gaccguguuc gaggccaacc agaaggacuu ccgcccccag     3060

ugggagcggg agaagcucgg cgggaagcug gucaugcgcc ugcacaaggg cgacgccgug     3120

gagcucgaga gcuccggcgu gcggcugguc aaguucgugc agcagaucug gaugaagucc     3180

aaccuggugc uccugacgga gcacaacgag ggcggggacc ugcagaagcg ccaccaggac     3240

gccgacgacc ccuuccggug ggacuucgcc aacaucggga agcucaagga gcgcggcugc     3300

gucgcgguga agguggacga gcuggggcgc gucaccaagc ggaucagcaa caccuga        3357


<210>  1614
<211>  3360
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1614
augggcuacc gcaucgggcu ggacgugggc aucaccagca ccggguacgc cguccucaag       60

acggacaaga acggccugcc cuacaagauc cugacccucg acuccgugau cuacccgcgg      120

gccgagaacc cccagaccgg ggcgagccug gccgagcccc gccggaucaa gcgcggccug      180

cggcgccgga cccgccggac gaaguuccgc aagcagcgga cccagcagcu guucauccac      240

uccggccucc ugagcaagcc cgagaucgag cagauccucg ccaccccgca ggccaaguac      300

uccguguacg agcugcgcgu cgccgggcug gaccggcgcc ucaccaacag cgagcuguuc      360

cgggugcugu acuucuucau cggccaccgc ggguucaagu ccaaccggaa ggcggagcuc      420

aaccccgaga acgaggccga caagaagcag augggccagc ugcugaacag caucgaggag      480

auccgcaagg ccaucgccga gaagggguac cggacggugg gcgagcucua ccugaaggac      540

cccaaguaca acgaccacaa gcgcaacaag ggcuacaucg acggguaccu guccaccccc      600

aaccggcaga ugcucgucga cgagaucaag cagauccugg acaagcagcg cgagcugggc      660

aacgagaagc ucaccgacga guucuacgcg accuaccugc ugggggacga gaaccgcgcc      720

ggcaucuucc aggcccagcg ggacuucgac gagggcccgg gggccggccc cuacgccggg      780

gaccagauca agaagauggu gggcaaggac aucuucgagc ccacggagga ccgcgcggcc      840

aaggccaccu acaccuucca guacuucaac cuccugcaga agaugaccag ccugaacuac      900

cagaacacga ccggggacac cuggcacacc cucaacggcc uggaccggca ggccaucauc      960

gacgccgugu ucgcgaaggc cgagaagccc acgaagaccu acaagccgac cgacuucggc     1020

gagcugcgca agcuccugaa gcugcccgac gacgcccggu ucaaccucgu caacuacggg     1080

ucccugcaga cccagaagga gaucgagacg guggagaaga agacccgcuu cguggacuuc     1140

aaggccuacc acgaccuggu caaggugcuc cccgaggaga uguggcagag ccggcagcug     1200

cuggaccaca ucggcaccgc ccucacccug uacuccagcg acaagcgccg gcgccgguac     1260

uucgcggagg agcugaaccu ccccgccgag cugaucgaga agcugcuccc gcugaacuuc     1320

uccaaguucg ggcaccugag caucaagucc augcagaaca ucauccccua ccuggagaug     1380

ggccaggugu acagcgaggc cacgaccaac accggguacg acuuccgcaa gaagcagauc     1440

uccaaggaca ccauccggga ggagaucacg aaccccgucg ugcgccgggc cgugaccaag     1500

accaucaaga ucgucgagca gaucauccgc cgguacggca agcccgacgg caucaacauc     1560

gagcucgccc gcgagcuggg gcggaacuuc aaggagcgcg gcgacaucca gaagcgccag     1620

gacaagaacc ggcagaccaa cgacaagauc gcggccgagc ugacggagcu cgggaucccg     1680

gugaacggcc agaacaucau ccgcuacaag cugcacaagg agcagaacgg gguggacccc     1740

uacaccggcg accagauccc cuucgagcgg gccuucagcg agggcuacga ggucgaccac     1800

aucauccccu acagcaucuc cugggacgac agcuacacca acaaggugcu gaccuccgcc     1860

aagugcaacc gcgagaaggg gaaccggauc ccgauggugu accucgcgaa caacgagcag     1920

cgccugaacg cccugaccaa caucgccgac aacaucaucc ggaacagccg caagcggcag     1980

aagcuccuga agcagaagcu guccgacgag gagcucaagg acuggaagca gcgcaacauc     2040

aacgacacgc gguucaucac ccgcguccug uacaacuacu uccggcaggc caucgaguuc     2100

aaccccgagc uggagaagaa gcagcgcgug cucccccuga acggcgaggu caccagcaag     2160

auccgguccc gcuggggguu ccugaaggug cgggaggacg gcgaccucca ccacgccauc     2220

gacgcgaccg ugaucgccgc caucacgccc aaguucaucc agcaggucac caaguacagc     2280

cagcaccagg aggugaagaa caaccaggcc cuguggcacg acgccgagau caaggacgcg     2340

gaguacgccg ccgaggccca gcgcauggac gccgaccugu ucaacaagau cuucaacggc     2400

uucccgcucc ccuggcccga guuccuggac gagcugcucg cgcggaucuc cgacaacccg     2460

guggagauga ugaagucccg cagcuggaac accuacaccc ccaucgagau cgccaagcug     2520

aagcccgucu ucguggugcg gcuggccaac cacaagauca gcgggcccgc ccaccucgac     2580

acgauccgcu ccgcgaagcu guucgacgag aagggcaucg uccugagccg cguguccauc     2640

accaagcuca agaucaacaa gaagggccag guggccaccg gggacgggau cuacgacccg     2700

gagaacagca acaacggcga caaggucgug uacuccgcca uccggcaggc ccuggaggcc     2760

cacaacggca gcggggagcu ggcguucccc gacggcuacc ucgaguacgu ggaccacggg     2820

accaagaagc ugguccgcaa ggugcgggug gccaagaagg ucucccugcc cgugcgccuc     2880

aagaacaagg ccgccgccga caacggcagc auggugcgga ucgacgucuu caacacgggg     2940

aagaaguucg uguucgugcc caucuacauc aaggacaccg ucgagcaggu ccugccgaac     3000

aaggcgaucg cccgcggcaa gagccugugg uaccagauca ccgaguccga ccaguucugc     3060

uucagccucu accccggcga cauggugcac aucgagucca agaccgggau caagcccaag     3120

uacagcaaca aggagaacaa cacguccgug gugcccauca agaacuucua cggcuacuuc     3180

gacggggccg acaucgccac cgccagcauc cuggugcggg cgcacgacuc cagcuacacc     3240

gcccgcucca ucggcaucgc cgggcugcuc aaguucgaga aguaccaggu cgacuacuuc     3300

ggccgguacc acaaggugca cgagaagaag cgccagcugu ucgugaagcg ggacgaguga     3360


<210>  1615
<211>  3360
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1615
augaacgaca agaagaaccu gggcccccuc accuucgggu ucgacaucgg caucgccagc       60

gugggguggg ccguccuguc cccgacccgc aucguggacc ugggcgugcg gugcuucgac      120

aaggcggaga cggccaagga gggggagagc cucaaccugg cccgccgguc cgcccgccug      180

augcggcgcc ggcuccgccg gcgcgccugg cggcugauca agcuggcgcg ccuccugaag      240

cacgagggcc ugaucgccga caccaagacc uucauccagc ccagcgccuc ccacgccagc      300

cccuggcagc uccggaagga cggccuggac cgcgcgcugu cccccgagga gugggcccgg      360

guccucuacc accugugcaa gcaccgcggg uuccacuggg ugagccgggc cgaggcccgc      420

aaggccgacg gcgacaccaa gggggagggc gggaagguga agcagggccu ggcguccacg      480

accaagcuca ugaacgacaa gagcuaccgg accgccgccg agaugguccu ggccgaguuc      540

ccguccgccc agcgcaacaa gcggggcgag uacaccaagg cgcugagccg cgagcuccug      600

gacaacgagc ugaagcagcu guucgagcgc cagcgggagu acgggaaccc ccacgccgac      660

acgaaccucg aggccaagau ccgcggcucc ggggaccaca agagcggccu guucugggag      720

cagaagcccg cccucuccgg caacgaccug cugaagaugc ucgggaagug caccuucgag      780

aagcaggagu accgggcccc caaggcgagc uucaccgccg agcgccacgu guggcugacc      840

cggcugaaca accuccgcau caucguggac ggccggacgc gcccgcuggc cgacgccgag      900

cggaccaugg cccugucccu ccccuacgag caggucgggg acuucaccua caagcagcug      960

cgcgcggccc uggugaaggc cggccucaug cccgacagcu ucaaguucgc cucccuggcg     1020

uaccccagcg aguuccagaa ggccgagggg aaggccaagg acccggagag cgccgcccug     1080

accaagcucg cggccuggca ggagcugcgg gccacgcuga agaaggccga ccucgagucc     1140

gaguggcagc agaucagcug cgccgcgcug gagggccagc ccgagacccu ggacgagauc     1200

gccggcgugc ucuccgucuu caaggacgac gacgaggugc ugaacgagcu gcagaagcug     1260

gggcuccccg gcggggccaa gaccgagaac gcccugcugg acauccgcuu cgacaaguuc     1320

cacgcccuca gccugaaggc gcugcggaag aucgugcccu acauggagcg cggccuccgg     1380

uacgacgagg ccugcgagcg cgccgaguac caccacuccc agcugcacaa ggucggggag     1440

ggcaaccgga aguaccugcc gccccucuac aagagccgcg acaaggacgg ccggauggug     1500

uucaacgacg agcuggggga caucccccgc aaccccgugg uccugcgcuc ccucaaccag     1560

gcccggaagg ugcugaacgc gcugaucgcc cgcuacggca gcccguccag cgugcacauc     1620

gagauggccc gggagcucuc caagcccuuc gacgagcgca ugaaggucgg gaaggagcag     1680

gaggccuacc gggagcgcaa cgagaaggac aagcagaccu ucguggccga guucggcauc     1740

gugggggcgg ccaagggcgg cgaguucgag aaguggaagc uguaccggga gcagcagggg     1800

aagugcgccu acagcaucca gggcaucgac cugaaccgcc uccuggagcc cggguacguc     1860

gagaucgacc acgcccugcc cuacucccgg agcuucgacg acuccaagag caacaaggug     1920

cucguccugg ccgcggagaa ccgcaacaag ggcaaccgga cgccguacga guaccuggac     1980

ggcgagcaca acuccgagcg cuggcggcag uucguggggu ucguggagag caacaagcuc     2040

uaccgccagg ccaagcgguc ccgccugcug cggaaggacu ucggcaagga gcaggccgac     2100

gaguuccgcg agcggaaccu caacgacacc cgcuacgucu gccgguucuu caagaaccag     2160

guggagcagu uccugaagcu ggccgagggg agcgagagca agcgcugcgu gguccucucc     2220

ggccagcuga ccgccuuccu gcgcgcgcgg ugggggcuca ucaaggugcg cgacgagagc     2280

gaccggcacc acgcccugga cgccgugguc guggccgcgu gcacccacgg cauggugaag     2340

cgcauguccg acuacgcccg gcgcaaggag cuggaccagg ucgccgaggg cuucguggac     2400

guggagacgg gggagaucau caacgccgcc auguuccagc agcuccacga gcacuucccc     2460

gaccccuggc cccacuuccg ggacgagguc cagaugcgcc ugaagaucga cgacccggcg     2520

cagcugcgga gcgaccuccu gcgccugggc aacuacgccg aggaggacgu gcacgccgug     2580

cggccccucu ucgucucccg cgcgccccag cggcgcaaca gcggggccgc ccacaaggag     2640

accaucuacg gccagccgga gcggcugaag gcccagggcg gggugaccga gaagguggcc     2700

cugaccgccc ucacgaugaa ggaccuggac cgccugaucg acccccaccg gaacgagaag     2760

cucuacgccg cgauccgcaa gcggcuggag gaguucggcg ggaaggccga caaggccuuc     2820

gccgccucca acccccugca caagcccgac aaggagggca acccgaccgg gcccaucguc     2880

cgcaccguga cccucgagau cgacaagcug agcggcgugc ccguccgcgg gggcaucgcg     2940

aagaacgaca cgaugcugcg gguggacgug uucaccaagg ccggcaaguu ccaccucguc     3000

cccguguacg ugcaccacaa ggucaccggg cugccgaacc gcgccaucgu ggccuccaag     3060

gacgaggacg aguggaccgc gaucgacgac agcuucgagu uccuguucuc ccucuacccc     3120

aacgaccugg ugacgaucac ccagaagggc aaggcccccg uccgggggua cuacgccagc     3180

ugccaccgcg gcaccggggc cuucucccug ugggcccacg accgggcgag cuccgugggc     3240

aagggcgggc agaucgaggg caucgggauc aagaccgccc ucgccgugca gaaguacaac     3300

gucgacgugc ugggcaccac guacgccgcg ccccuggagc agcgccgggg ccucgccuga     3360


<210>  1616
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1616
augcgcaagg ugaccaucgg cuucgacauc ggggucagcu ccgugggcug gagcgugguc       60

gacaugaaca acggggccau cguggagacc ggcgugcggc uguucuccgc cagcuccgcg      120

gagaacaacg ucgagcgccg gaacaaccgc cagacgcggc gccucaaccg gcgccggaag      180

aaccgccuga aggacgugaa guaccugcuc gagaccaacc acuucaccuu cgaccaggag      240

caggggacca accccuacga gcugcgggug gccggccugc accgcaagcu cacgcggagc      300

gagcuggccc gcgcccugua ccaccucguc aagcggcgcg gcaucuccua cgaccugacc      360

gacaucgagg aggaggagau cgugaccacc agcgccuaca agacggcgcu ggggaagaac      420

cagucccagc ucgccgagaa gaccccgggc gagauccagu ucgcccggcu ggagaacuac      480

gggaaggugc gcggcagcau cgagaucaac gagcaggaca uccugcucaa caucuucccc      540

accuccgccu accugaacga ggcgaagcgg auccuggcca cccagcgcaa guucuacccc      600

gagaucacgg aggccuucau cgagaaguac ugcaccaucc ucacccggaa gcgcgaguac      660

uacgucgggc ccggcaccga gaagaaccgc acggacuacg gcaucuaccg gaccaacggg      720

gagacccugg acaaccuguu cgagauccuc aucggcaagg acaaguucua cccgaacgag      780

gagcgcgccg ccgggaacag cuacaccgcg cagcuguuca acuucgugaa cgaccugaac      840

aaccucaaga uccagacgau cgaggaccag aagcugaccc ccaagcagaa ggaggagauc      900

aucgagcagg ucaagaccgc cgagaagaag gugucccuga uccagcucau cagcaagauc      960

accaagacgc ccaaggacca gaucaccggc uaccggaccg acaagaacgg caagcccgac     1020

cugcacucca uggccguguu ccgcaaggug cggaagcagu uccugaccau ggagaucgac     1080

auccuccacu ggccggacga gcugcugaac gaccucgggc gccugcugac gcucaacacc     1140

gagaagggcg agauccggaa gcagcugacc aaggacuuca ugaagcgcua ccccauccug     1200

aacgagcagc ucgucgacca gaucaucgag aaccggcaca ucuucgccgu gagcgggaac     1260

cacaaguggc accgcuucuc ccugcggacc augaacgagc ugcuccccga gaugcuggcc     1320

acgagcaagg agcagaugac ccugcucucc gagcgcggca ugaucaagca gcacaagcgg     1380

gacuaccuga agaugaacga gcuggacgcg cgccucauca gcgaggagau cuacaacccc     1440

guggucggca agagcgugcg ggaggccauc aagcuguuca acgagcugau gaagaaguac     1500

caggaggugc gcuacgucgu gcuggagaug ccgcgggacg acaacgagga gaaggccaag     1560

aaggagcucc agaaguacca gaaggagaac gagaacgaga aggagaucgc ccuguccgag     1620

uuccagcgca agggguucuu cagcgacgcc cagcuggagc gggcgcucau caaggaccgc     1680

aagcugaaga ccaagauccg cuucuggcac cagcaggagg gcaagugccu guacuccggg     1740

cagaucauca cccccgucga gcuccugacg aacagcuucu ccuucgacau cgaccacauc     1800

aucccccaga gcguguccuu cgacgacagc cugaacaaca aggugcucug caccaaggag     1860

augaacgacc agaagggcaa gcagaccccc uacggguucc ugcugaacgg caacgggcag     1920

ggcuucgacc gguucaaggc cauggucaac gccaaccgcc ucauguccaa gggcaagaag     1980

aagaaccugc uguucaugga ggaccucaac aacaucgaga cccggaagcg cuucaucgcc     2040

cggaaccugg uggacacccg cuacgccagc cggguggucc ugaacgagau ccagcaguac     2100

cuccgcgcga agaacaagga cacccaggug gccguggucc gggggaaguu cacggccacc     2160

cugcgcaagc acuggaacau caacaagucc cgggagaccc accaccacca cgcccuggac     2220

gcgagcauca ucgccaucac gccgcuccug uccgugugga agaagaagca gacccugauc     2280

cccaagaccc ucagcgagga gaagcuggag uucgaguacg acgacaucgu ggaggacacc     2340

aucuucgaga aggcccugua ccagcucccc aaggagaacu ucaaggagca gcugaacaac     2400

ucccagccca acaucaaguu cagcuaccag gucgacaaga agaugaaccg caaggugucc     2460

gacgccacga ucuacagcgu gaagcaggcc aagcugucca gcgacaagca gagcgaggag     2520

uacguccucg cgaagaucaa ggacaucuac uccguggagg gcuacaagac cuucgccaag     2580

aucuacgaga aggacaagag caaguuccug auggcccaga acgacccgaa gaccuucgag     2640

aagcuggagg agaucaucca guccuacccc agcacccggg agaucaucgu gggggagaag     2700

gucaaggugg uggacgucuc ccccuucgag cucuaccgca aggaguacgg cuacaucacg     2760

aaguacgcca agaagaacaa cgggcccauc auccggcagc ugaaguacua cgaccacaag     2820

aucggcaugc accuggacau caccaaccag aagaacaacg gcaaggggaa gaaggugauc     2880

cuccagcagc ucaagagcug gcgcaccgac guguacuaca accacgagaa gcaggaguac     2940

gagaucaugg gccugaagua cuccgaccug cgguucaaga aggaccagua cgggaucccg     3000

aaggagcgcu accaggaggu caagcagaug gagaagaucg cgccccagag cgaguucugc     3060

uucucccugu accggaacga ccgcaucaag gugcugaaca aggagaccga cgaggaggug     3120

gagcuccugu ucggcgcccg gaacuacacg agcgccggcu acgucgaccu gaagcccauc     3180

gaccgcgugg aguucgggaa gaaggagugg auccccgugu accaguacgc caccuccgac     3240

ggcaagugca agaagaaguu cgccaagaag ggguacgaga ucuacaaggu caacaccacc     3300

auccucggcg agagcuacua caucaagaag gagcgcaugc ugccgaagaa cauccuggac     3360

uga                                                                   3363


<210>  1617
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1617
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucguccggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgccggg ugcgccugaa ccgccucuuc gaggagaucg gccugaucac cgacuucacc      240

aagaucucca ucaaccugaa cccguaccag cuccggguga aggggcugac cgacgagcug      300

agcaacgagg agcuguucau cgcccugaag aacaugguca agcaccgggg caucuccuac      360

cuggacgacg ccagcgacga cgggaacucc agcgugggcg acuacgccca gaucgugaag      420

gagaacucca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgcuaccag      480

accuacggcc agcugcgggg cgacuucacc gucgagaagg acgggaagaa gcaccgccuc      540

aucaacgugu uccccacgag cgccuaccgg uccgaggcgc ugcgcauccu gcagacccag      600

caggaguuca acccccagau caccgacgag uucaucaacc gguaccucga gauccugacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggacgga cuacggccgc      720

uaccgcacca gcggggagac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuaccccg acgaguuccg ggccgccaag gccagcuaca cggcgcagga guucaaccug      840

cugaacgacc ucaacaaccu gaccgugccc accgagacca agaagcuguc caaggagcag      900

aagaaccaga ucaucaacua cgucaagaac gagaaggcca ugggccccgc caagcuguuc      960

aaguacaucg ccaagcuccu gagcugcgac guggccgaca ucaaggggua ccgcaucgac     1020

aaguccggca aggcggagau ccacacguuc gaggccuacc ggaagaugaa gacccucgag     1080

acccuggaca ucgagcagau ggaccgcgag acccuggaca agcucgccua cgugcugacg     1140

cugaacaccg agcgggaggg cauccaggag gcccucgagc acgaguucgc cgacgggagc     1200

uucucccaga agcaggucga cgagcuggug caguuccgca aggcgaacag cagcaucuuc     1260

ggcaaggggu ggcacaacuu cuccgugaag cugaugaugg agcucauccc ggagcuguac     1320

gagaccagcg aggagcagau gaccauccug acgcggcucg gcaagcagaa gaccaccucc     1380

agcuccaaca agaccaagua caucgacgag aagcugcuga cggaggagau cuacaacccc     1440

gucguggcca agagcgugcg ccaggccauc aagaucguca acgccgccau caaggaguac     1500

ggggacuucg acaacaucgu gaucgagaug gcgcgggaga ccaacgagga cgacgagaag     1560

aaggccaucc agaagaucca gaaggccaac aaggacgaga aggacgccgc caugcucaag     1620

gcggccaacc aguacaacgg caaggccgag cugccccacu ccguguucca cggccacaag     1680

cagcuggcca ccaagauccg ccuguggcac cagcaggggg agcggugccu cuacaccggc     1740

aagacgauca gcauccacga ccugaucaac aaccccaacc aguucgaggu cgaccacauc     1800

cugccgcucu ccaucaccuu cgacgacagc cuggcgaaca aggugcuggu cuacgccacc     1860

gccaaccagg agaaggggca gcgcaccccc uaccaggccc ucgacuccau ggacgacgcc     1920

uggagcuucc gggagcugaa ggcguucgug cgcgagucca agacccugag caacaagaag     1980

aaggaguacc uccugacgga ggaggacauc uccaaguucg acgugcggaa gaaguucauc     2040

gagcgcaacc uggucgacac ccgguacgcc agccgcgugg ugcucaacgc ccugcaggag     2100

cacuuccggg cccacaagau cgacaccaag gucuccgugg ugcgcggcca guucaccagc     2160

cagcugcgcc ggcacugggg gaucgagaag acgcgcgaca ccuaccacca ccacgccguc     2220

gacgcgcuca ucaucgccgc cuccagccag cugaaccugu ggaagaagca gaagaacacc     2280

cucgugagcu acuccgagga ccagcugcug gacaucgaga ccggcgagcu caucagcgac     2340

gacgaguaca aggaguccgu guucaaggcc cccuaccagc acuucgucga cacgcucaag     2400

agcaaggagu ucgaggacuc cauccuguuc agcuaccagg uggacuccaa guucaaccgg     2460

aagaucagcg acgccaccau cuacgcgacc cgccaggcca aggugggcaa ggacaaggcc     2520

gacgagaccu acguccucgg gaagaucaag gacaucuaca cgcaggacgg cuacgacgcc     2580

uucaugaaga ucuacaagaa ggacaagucc aaguuccuga uguaccggca cgacccccag     2640

accuucgaga aggugaucga gccgauccug gagaacuacc ccaacaagca gaugaacgag     2700

aaggggaagg aggugcccug caaccccuuc cucaaguaca aggaggagca cggcuacauc     2760

cgcaaguaca gcaagaaggg caacgggccg gagaucaagu cccugaagua cuacgacagc     2820

aagcugggca accacaucga caucaccccc aaggacucca acaacaaggu cgugcuccag     2880

agccugaagc ccuggcggac cgacguguac uucaacaaga acacggggaa guacgagauc     2940

cugggccuca aguacuccga caugcaguuc gagaagggga ccggcaagua cagcaucucc     3000

aaggagcagu acgagaacau caagguccgc gagggcgugg acgagaacag cgaguucaag     3060

uucacccugu acaagaacga ccugcuccug cugaaggaca gcgagaacgg ggagcagauc     3120

cuccugcggu ucaccucccg caacgacacg agcaagcacu acguggagcu gaagcccuac     3180

aaccggcaga aguucgaggg cuccgaguac cucaucaaga gccuggggac cgucgugaag     3240

ggcgggcgcu gcaucaaggg ccugggcaag uccaacauca gcaucuacaa ggugcggacc     3300

gacguccucg ggaaccagca caucaucaag aacgagggcg acaagccgaa gcuggacuuc     3360

uga                                                                   3363


<210>  1618
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1618
augggccugg acaucggggu ggccagcguc ggcuucuccg ugcucgacau cgacaagggg       60

aaggugaucg agcugggcgc ccgccuguuc agcgcgaccg ucgccgccgg gaaccaggac      120

cggcgcgaca ugcggggcgc ccgccggcuc cugaaccgca acaagcagcg gcgccaggac      180

accggcaagc uguucaagaa guucgggcuc aucgacgacu ucgacaaggg cuccuucuac      240

gacaacuuca accagaaccu gaaccccuac gagcugcggg ugaaggggcu cacggagcag      300

cugaccaagg aggagcuggc cgagagccuc uaccagaucg ugaagcaccg cggcaucucc      360

uacgcgcuga aggacgccga cgucgacgag ggcgggaccg acuacagcgu gucccugaag      420

aucaacagcc aggagcucgc cgagaagacc ccggcccaga uccagcugca gcggcugaac      480

gacuacggca aggugcgcgg gcaggucgug aucggcgacg accccgacaa ccagaaggug      540

cuccugaacg ucuuccccac guccgcguac gagaaggagg ccaagcagau caucgccacc      600

cagcagcagu ucuaccccga gagccugacc gacaaguuca ccgaggagua cugccagauc      660

cucacgcgga agcgcgacua cuucgugggg ccgggcaacg agaagucccg gaccgacuac      720

ggcaucuaca agaccgacgg gcgcacccug gacaaccugu ucgaggagcu caucggccac      780

gacaagaucu accccgagga gcugcgggcc agcgccgcgu ccuacacggc ccagcuguuc      840

aacgugcuca acgaccugaa caaccugcgc auccucaacu acgaggacgg gaagcugacc      900

aaggaggaca aggagaagau caucgccgag aucaagaaca acaccaccac gaucaacaug      960

cugaacguca ucaagaaggu ggccggcugc agcaaggacg acaucaaggg guuccgggug     1020

aacgagaagg acaagcccga gaucuccagc augcccgucu accgcaagau ccacaaggac     1080

cuccugaagg ccggcgugga caucuccgac uggccggugg aguucaucga cgagcugagc     1140

uucauccuca cccugaacac cgagaacggc gagauccgga agcagcugaa caaccgccuc     1200

gcgcccaagu ucgacuuccu gaacgccgac cugauccagc ucaucaucga caacaaggac     1260

uccuucgaga ucaagaccaa caacaagugg caccgcuuca gcgucaagac gaugaacaag     1320

cugauccccg agaugaugga gcggcccgug gagcagauga cccugcucaa cgagaugggg     1380

cuggugaaga aggacaagaa gcgcuucgag aacaacaagu accugccgua caaggagauc     1440

gccaaggaca ucuucaaccc cgucgccagc aaguccgugc gggaggcccu caagaucguc     1500

aacgcggugc ugaagaagua cggccacauc gacuaccugg uggucgagau gccccgcgac     1560

aagaaccuga aggaggagca ggacaacauc aaggaguucc agaacaagaa caagaaggcc     1620

aaggacgccg ccuucgaggc cuucgucaag agcguggggu ccgagcagcg ggugaaggag     1680

gcgcucagca agaaccgcaa gcugcagaug aagaugcggc ugugguacca gcagcaggag     1740

aucgaccccu acaacggcaa gaccaucgac gccaccgacc ucaucaacaa cccggacaag     1800

uucgagaucg accacaucau cccccagucc aucagcuucu acgacuccau caacaacaag     1860

acgcugugcu ucgccagcau gaaccaggug aaggggcaga agacccccua cgaguucaug     1920

cuggagggcc acggccaguc cuacgacaag uucaaggcca ccgugauggc gaacaagaac     1980

uucgggaagg ccaagcgcgc caacuaccuc uucgaggaga acgucagcga caucgagacc     2040

cggaagcgcu uccugucccg gaaccuggug gacacgcgcu acagcucccg ggugguccuc     2100

aacagccugc aggacuucuu ccgcgagaag uccgccgaca ccaaggugac cgugauccgg     2160

ggcaaguuca ccagcaacau gcgcaagcac uggcacaucg acaagacgcg ggagaccuuc     2220

caccaccacg ccaucgacgc guccaucauc gccgccaccc ccuuccugcg cauguggaag     2280

aagggcggga ccaucuuccc ggucaaggug ggggaggaga gcaucgacau cgagacgggc     2340

gagauccucg acgacaagaa cuucgacaag gccauguacg aggagcccua cuccggcuuc     2400

gugagcgaga ucaugaacgc cgacgaccgg aucaaguuca gccaccaggu cgacaagaag     2460

augaaccgca agguguccga cgcgaccauc uacagcaccc gcaccgggaa gcuggccaag     2520

gacaagaagg acgccgagua caucguggcc aaggucaagg acaucuacuc cguggacggc     2580

uucaagaagu ucaagaaggu guacgacaag gacaagacca aguuccugcu cuacaaguac     2640

gacccccgga cguucagcaa gcuggagcgc aucaucuccg acugccccga caaggucgag     2700

aaggugcaga ccaacgggaa ggugaaggcc gucgacauca gcccguucga gauguaccgg     2760

cgcgaccacg gcaugaucaa gaaguacucc aagaagggca acgggcccgc gaucaagcag     2820

cugaaguacc ucgacaagaa gcugggcagc cacaucgaca ucacccccgc caacgccaac     2880

gggaagcacg ugauccugca gucccucaag cccuggcgga ccgacgugua ccugaaccac     2940

gagacgggcg aguacgagau cauggggauc aaguacagcg accugaaguu caacaagaac     3000

gagggcuacg gcaucaagaa ggacaaguac cucgagauca agaaggucga ggaggugucc     3060

gagaaguccg aguucauguu cagccuguac cgcaaggacc gggugaaggu ccaggacaug     3120

aagaccgggg agagcgugga gcuccuguuc uggucccgca acuucagcaa caagaaguac     3180

gccgagcuga agccgaucuc ccaggcggag aacgacaaga agcugcccgu guacggcaag     3240

gggcggcuca ucaagcgccu gauccccaag aacugcaaga ucuggaaggu caacaccacc     3300

auccugggcg acccguacua ccucgagaag gagagcgaca gccccaagga cauccuggac     3360

uga                                                                   3363


<210>  1619
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1619
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucguccggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgccggg ugcgccugaa ccgccucuuc gaggaguccg gccugaucac cgacuucacc      240

aagaucagca ucaaccugaa cccguaccag cuccggguga aggggcugac cgacgagcug      300

uccaacgagg agcuguucau cgcccugaag aacaugguca agcaccgggg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccaucggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgcuaccag      480

accuacggcc agcugcgggg cgacuucacc guggagaagg acgggaagaa gcaccgccuc      540

aucaacgucu uccccacguc cgccuaccgg agcgaggcgc ugcgcauccu gcagacccag      600

caggaguuca acccccagau caccgacgag uucgugaacc gguaccucga gauccugacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggacgga cuacggccgc      720

uaccgcacca acggggagac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuaccccg acgaguuccg ggccgccaag gccagcuaca cggcgcagga guucaaccug      840

cugaacgacc ucaacaaccu gaccgugccc accgagacca agaagcuguc caaggagcag      900

aagaaccaga ucaucaacua cgucaagaac gagaaggcca ugggccccgc caagcuguuc      960

aaguacaucg ccaagcuccu gagcugcgac guggccgaca ucaaggggua ccgcaucgac     1020

aaguccggca aggcggagau ccacacguuc gaggccuacc ggaagaugaa gacccucgag     1080

acccuggaca ucgagcagau ggaccgcgag acccuggaca agcucgccua cgugcugacg     1140

cugaacaccg agcgggaggg cauccaggag gcccucgagc acgaguucgc cgacgggagc     1200

uucucccaga agcaggucga cgagcuggug caguuccgca aggcgaacag cagcaucuuc     1260

ggcaaggggu ggcacaacuu cuccgugaag cugaugaugg agcucauccc ggagcuguac     1320

gagaccagcg aggagcagau gaccauccug acgcggcucg gcaagcagaa gaccaccucc     1380

agcuccaaca agaccaagua caucgacgag aagcugcuga cggaggagau cuacaacccc     1440

gucguggcca agagcgugcg ccaggccauc aagaucguca acgccgccau caaggaguac     1500

ggggacuucg acaacaucgu gaucgagaug gcgcgggaga ccaacgagga cgacgagaag     1560

aaggccaucc agaagaucca gaaggccaac aaggacgaga aggacgccgc caugcucaag     1620

gcggccaacc aguacaacgg caaggccgag cugccccacu ccguguucca cggccacaag     1680

cagcuggcca ccaagauccg ccuguggcac cagcaggggg agcggugccu cuacaccggc     1740

aagacgauca gcauccacga ccugaucaac aacuccaacc aguucgaggu cgaccacauc     1800

cugccccuca gcaucaccuu cgacgacucc cuggcgaaca aggugcuggu cuacgccacc     1860

gccaaccagg agaaggggca gcgcaccccg uaccaggccc ucgacagcau ggacgacgcc     1920

ugguccuucc gggagcugaa ggcguucgug cgcgagagca agacccuguc caacaagaag     1980

aaggaguacc uccugacgga ggaggacauc agcaaguucg acgugcggaa gaaguucauc     2040

gagcgcaacc uggucgacac ccgguacgcc ucccgcgugg ugcucaacgc ccugcaggag     2100

cacuuccggg cccacaagau cgacaccaag gucagcgugg ugcgcggcca guucaccucc     2160

cagcugcgcc ggcacugggg gaucgagaag acgcgcgaca ccuaccacca ccacgccguc     2220

gacgcgcuca ucaucgccgc cagcagccag cugaaccugu ggaagaagca gaagaacacc     2280

cucguguccu acagcgagga ccagcugcug gacaucgaga ccggcgagcu caucuccgac     2340

gacgaguaca aggaguucgu guucaaggcc cccuaccagc acuucgucga cacgcucaag     2400

agcaaggagu ucgaggacuc cauccuguuc agcuaccagg uggacuccaa guucaaccgg     2460

aagaucagcg acgccaccau cuacgcgacc cgccaggcca aggugggcaa ggacaaggcc     2520

gacgagaccu acguccucgg gaagaucaag gacaucuaca cgcaggacgg cuacgacgcc     2580

uucaugaaga ucuacaagaa ggacaagucc aaguuccuga uguaccggca cgacccccag     2640

accuucgaga aggugaucga gcccauccug gagaacuacc cgaacaagca gaugaacgag     2700

aaggggaagg aggugcccug caaccccuuc cucaaguaca aggaggagca cggcuacauc     2760

cgcaaguaca gcaagaaggg caacgggccc gagaucaagu cccugaagua cuacgacagc     2820

aagcugggca accacaucga caucaccccg aaggacucca acaacaaggu cgugcuccag     2880

agcguguccc ccuggcgggc ggacgucuac uucaacaaca ccacggggaa guacgagauc     2940

cugggccuga aguacgccga ccuccaguuc gagaagggga ccggcaccua caagaucagc     3000

caggagaagu acaacgacau caagaagaag gagggcgugg acuccgacag cgaguucaag     3060

uucacccugu acaagaacga ccugcuccug gugaaggaga cggagaccaa ggagcagcag     3120

cuguuccgcu uccucagccg gaccaugccc aagcagaagc acuacgucga gcugaagccc     3180

uacgacaagc agaaguucga gggcggggag gcccugauca aggugcucgg gaacguggcc     3240

aacggcgggc agugcaucaa gggccugggc aaguccaaca ucagcaucua caagguccgc     3300

accgacgugc uggggaacca gcacaucauc aagaacgagg gcgacaagcc gaagcucgac     3360

uucuga                                                                3366


<210>  1620
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1620
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaagaacu ccgggaagau cauccacgcc aacagccgcc ucuuccccgc ggccaccgcc      120

gacaacaacg uggagcggcg caagaaccgg caggcccgcc ggcugaaccg ccggaagaag      180

caccgcggcg uccgguucca ggaccuguuc gaggacuacg gccuccugac cgacuucucc      240

aaggugagca ucaaccugaa cccguaccgc cuccgggugg acgggcugaa caagcagcug      300

acgaacgagg agcuguucau cgcccucaag aacaucguca agcgccgggg caucuccuac      360

cuggacgacg cgagcgagga cgggggcacc guguccagcg acuacgggaa ggccguggag      420

gagaaccgca agcuccuggc cgagcagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc agcuccgcgg ggacuucacc gucguggaga acggcgagaa gugccggcug      540

aucaacgugu ucuccacgag cgccuacaag aaggaggcgg agcgcauccu gcggaagcag      600

caggaguuca acuccaagau caccgacgag uucaucgagg acuaccucaa gauccugacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccggaccga cuacgggcgc      720

uuccgcaagg acggcacgac ccuggacaac aucuucggga uccucaucgg caagugcacc      780

uucuacccca acgaguaccg ggccagcaag gccagcuaca ccgcccagga guucaaccug      840

cugaacgacc ucaacaaccu guccgucccg acggagacca agaagcugag cgaggagcag      900

aagaagacca ucaucgagua cgccaagucc gcgaagaccc ucggggccag cacgcugcug      960

aaguacaucg ccaagaugau cgacaccucc guggaccaga uccgcggcua ccggguggac     1020

gucaacaaca agcccgagau gcacaccuuc gagguguacc gcaagaugca gagccucgag     1080

accaucagcg ugggcgagcu gucccggaac guccuggacg agcucgccca cauccugacg     1140

cugaacaccg agcgcgaggg gaucgaggag gccaucaaca ccaagcucaa ggacagcuuc     1200

ucccaggacc aggugcugga gcuggugcag uuccggaaga acaacagcuc ccucuucagc     1260

aagggcuggc acaacuucuc ccugaagcug augauggagc ucauccccga gcuguacgag     1320

accagcgagg agcagaugac gauccugacc cgccucggga agcagaaguc caaggagacc     1380

agcaagcggu ccaaguacau cgacgagaag gagcugaccg aggagaucua caaccccguc     1440

guggccaaga gcgugcgcca ggcgaucaag aucaucaacg aggccacgaa gaaguacggc     1500

aucuucgaca acaucgucau cgagauggcc cgggagaacg acgaggagga cgccaagaag     1560

gacuacauca agcgccagaa ggcgaaccag gacgagaaga acgccgccau ggagaaggcc     1620

gccuuccagu acaacgggaa gaaggagcug ccggacaaca ucuuccacgg ccacaaggag     1680

cuggcgacca agauccggcu cuggcaccag cagggcgaga agugccugua caccgggaag     1740

accaucccca ucuccgaccu gauccacaac caguacaagu acgagaucga ccacgugcuc     1800

ccccugagcc uguccuucga cgacagccuc uccaacaagg uccuggugcu ggccacggcc     1860

aaccaggaga agggccagcg cacccccuuc caggcccucg acagcaugga cgacgggugg     1920

uccuaccggg aguucaagag cuacgugaag gagagcaagc ugcugggcaa caagaagaag     1980

gaguaccucc ugaccgagga ggacaucucc aagaucgagg ucaagcagaa guucaucgag     2040

cgcaaccugg uggacaaccg guacagcucc cgcguggucc ucaacgcgcu gcaggacuuc     2100

uacaaggccc acaaguucga caccacgauc agcguggugc gggggcaguu caccucccag     2160

cugcgccgca aguggggccu cgagaagucc cgggagaccu accaccacca cgccgucgac     2220

gcccugauca ucgccgcgag cagccagcug cgccucugga agaagcacaa caacccgcug     2280

aucuccuaca aggagggccg guucaucgac agcgagaccg gggagaucau cucccugagc     2340

gacgacgagu acaaggagcu cguguucaag gcccccuacg accacuucgu ggacacccug     2400

cgcuccaaga cguucgagga cagcauccug uucuccuacc aggucgacag caaguacaac     2460

cggaagaucu ccgacgccac caucuacgcc acccgcaagg ccaagcucga caaggacaag     2520

agcgaggaga ccuacgugcu gggcaagauc aaggacaucu acagccaggc gggguacgac     2580

gccuucauca agaucuacaa caaggacaag uccaaguucc ugauguacca caaggacccc     2640

cagacguucg agaaggugau cgaggagauc cuccggaccu accccagcaa ggagcugaac     2700

gacaagaaca aggagauccc gugcaacccc uucgagaagu accgccagga gaacggcccc     2760

auccggaagu acuccaagaa gggcaacggg cccgagauca agagcaugaa guacuacgac     2820

aagaacaucc cggaguccuu caucaacauc acccccagca acuccaacaa caagguccug     2880

cuccgccagc uguaccccug gcggaccgac guguacuuca acccccagac ggacaaguac     2940

gagcugcucg gccugaagua cgccgaccug caguucgaga aggccaccgg gaaguacagc     3000

aucaccaucg acaaguacaa cgagaucaag gagaaggagg gcguguccaa ggagagcgag     3060

uucaaguuca cccucuacaa gaacgaccug cugcucauca aggacacgaa gaacaacgag     3120

gagcagaucu uccgcuuccu gucccggacc augccgaacc agaaccacua cgucgagcug     3180

aagcccuacg acaaggggaa cuucgagggc aaggagaaga ucaagguguu caagaugacc     3240

agcaacggcc gcugcaucaa ggggcucggc aagaaguacc uguccaucua caaggugaag     3300

accgacaucc uggggaacaa gcacuucauc aagaaggagg gcgacgagcc caagcucaag     3360

uucuga                                                                3366


<210>  1621
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1621
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaagaacu ccgggaagau cauccacgcc aacagccgcc ucuuccccgc ggccaccgcc      120

gacaacaacg uggagcggcg caagaaccgg caggcccgcc ggcugaaccg ccggaagaag      180

caccgcggcg uccgguucca ggaccuguuc gaggacuacg gccuccugac cgacuucucc      240

aaggugagca ucaaccugaa cccguaccgc cuccgggugg acgggcugaa ccagcagcug      300

acgaacgagg agcuguucau cgcccucaag aacaucguca agcgccgggg caucuccuac      360

cuggacgacg cgagcgagga cgggggcgcc guguccagcg acuacgggaa ggccguggag      420

gagaaccgca agcuccuggc cgagcagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc agguccgcgg ggacuucacc gugguggaga acggcgagaa gcggcgccuc      540

aucaacgucu ucuccaccag cgcguaccgg aaggaggccg agaagauccu gcgcaagcag      600

caggaguuca acaaccagau cacggacgag uucaucgagg acuaccugaa gauccucacc      660

gggaagcgga aguacuacca cggccccggc aacgagaagu cccgcaccga cuacgggcgc      720

uuccggaccg acggcacgac ccuggacaac aucuucgggg ugcugaucgg caagugcacc      780

uucuacccca acgaguaccg cgccagcaag uccagcuaca ccgcccagga guucaaccuc      840

cugaacgacc ugaacaaccu cacggugccg accgagacca agaagcuguc cgaggagcag      900

aagaagguca ucguggagua cgccaaggag gcgaagaccc ugggggccag cacgcuccug      960

aaguacaucg ugaagaugau cgacgccucc gucgaccaga ucagcggcua ccggguggac     1020

gugaacaaca agcccgagau gcacaccuuc gaggucuacc gcaagaugca gucccuggag     1080

accaucagcg ugggcgagcu cagccggaac gugcuggacg agcuggccca cauccucacc     1140

cugaacacgg agcgcgaggg gaucgaggag gccaucaacu ccaagcugau gggcgucuuc     1200

agccaggacc aggugcucga gcugguccag uuccggaaga acaacuccag ccuguucucc     1260

aagggguggc acaacuucag ccucaagcug augauggagc ugauccccga gcucuacgag     1320

accuccgagg agcagaugac cauccugacc cgccugggca agcagaaguc caaggagacg     1380

agcaagcgga ccaaguacau cgacgagaag gagcucaccg aggagaucua caaccccgug     1440

guggccaaga gcguccgcca ggcgaucaag aucaucaacg aggccaccaa gaagcacggg     1500

aucuucgaca acaucgugau cgagauggcc cgggagaaca acgaggagga cgccaagaag     1560

gacuacauca agcgccagaa ggcgaaccag gacgagaaga acgccgccau ggagaaggcc     1620

gccuuccagu acaacggcaa gaaggagcug ccgggcaaca ucuucaacgg gcacaaggag     1680

cuggcgacga agauccggcu guggcaccag cagggcgaga agugccucua caccgggaag     1740

accaucccca ucuccgaccu gauccacaac caguacaagu acgagaucga ccacauccug     1800

ccccucagcc uguccuucga cgacagccug uccaacaagg ugcucgucca cgccaccgcc     1860

aaccaggaga agggccagcg cacgcccuuc caggcccugg acagcaugga cgacgcgugg     1920

uccuaccggg aguucaagag cuacgugaag gaccugaagu cccucagcaa caagaagaag     1980

gacuaccugc ugaccgagga ggacaucagc aagaucgagg ugaagcagaa guucaucgag     2040

cgcaaccucg ucgacacccg guacuccagc cgcguggugc ugaacgcccu gcaggacuuc     2100

uacaaguccc accaguucga caccacgauc agcgucgugc gggggcaguu caccucccag     2160

cuccgccgca aguggggccu ggagaagucc cgggagaccu accaccacca cgccguggac     2220

gcccugauca ucgccgcgag cagccagcuc cgccugugga agaagcagaa caacccgcug     2280

aucaccuaca aggagggcca guucaucgac ucccagaccg gggagaucau cagccucacg     2340

gacgacgagu acaaggagcu ggucuucaag gcccccuacg accacuucgu ggacacccug     2400

cacuccaaga ccuucgagga cggcauccuc uucagcuacc agguggacuc caaguacaac     2460

cggaagauca gcgacgccac caucuacgcc acgcgcaagg ccaagcugga caaggacaag     2520

uccgaggaga ccuacguccu ggggaagauc aaggacaucu acagccaggc gggcuacgac     2580

gccuucauca agaucuacaa caaggacaag cggaaguucc ucauguacca caaggacccc     2640

cagaccuucg agaaggugau cgaggagauc cugcgcaccu accccuccaa ggagcggaac     2700

gacaagaaca aggaggugcc gugcaacccc uucgagaagu accgccagga gaacggcccc     2760

auccggaagu acagcaagaa ggggaacggc cccgagauca agugccugaa guacuacgac     2820

aacaagcucg ggaacuacau cgacaucagc ccggacggcu ccgacaacca ggucgugcug     2880

cagagccuga agcccuggcg cacggacgug uacuucaacc accagaccaa gaaguacgag     2940

cucauggggc ugaaguacuc cgaccugagc uucgagaagg gcuccggcaa guacagcauc     3000

uccaacgaga aguacaacag caucaagcgg aucgaggggg ucaacgagca guccgaguuc     3060

aaguucaccc ucuacaagaa cgaccugauc cugaucaagg acagcgagaa caacgagcag     3120

aagcuguucc gcuucaacuc ccggaacgac accgccaagc acuacgugga gcucaagccc     3180

uacgacaagg cccgcuucga cggccagcag gagcugauca cgauccucgg gaacguggcc     3240

aagggcgggc agugccugaa gggccugaac aagagcaaca ucuccaucuu caaggucaag     3300

accgacgugc ucggcaagaa gcacaucauc aagaaggagg gggacgagcc caagcugaag     3360

uucuga                                                                3366


<210>  1622
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1622
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucguccggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgcaucg ugcggcugaa ccgccucuuc gaggaguccg gccugaucac cgacuucacc      240

aagaucagca ucaaccugaa cccguaccag cuccggguga aggggcugac cgacgagcug      300

uccaacgagg agcuguucau cgcccugaag aacaugguca agcaccgcgg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgguaccag      480

accuacggcc agcugcgcgg cgacuucacc gucgagaagg acgggaagaa gcaccggcuc      540

aucaacgugu uccccacguc cgccuaccgc agcgaggcgc ugcggauccu gaagacccag      600

caggaguuca acccccagau caccgacgag uucaucaacc gcuaccucga gauccugacc      660

ggcaagcgga aguacuacca cgggccgggc aacgagaagu cccgcacgga cuacggccgg      720

uaccgcacca acggggagac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuaccccg aggaguuccg cgccgccaag gccagcuaca cggcgcagga guucaaccug      840

cugaacgacc ucaacaaccu gaccgugccc accgagacca agaagcuguc caaggagcag      900

aagaaccaga ucaucaacua cgucaagaac gagaaggcca ugggccccgc caagcuguuc      960

aaguacaucg ccaagcuccu gagcugcgac guggccgaca ucaaggggua ccggaucgac     1020

aaguccggca aggcggagau ccacacguuc gaggccuacc gcaagaugaa gacccucgag     1080

acccuggaca ucgagcagau ggaccgggag acccuggaca agcucgccua cgugcugacg     1140

cugaacaccg agcgcgaggg cauccaggag gcccucgagc acgaguucgc cgacgggagc     1200

uucucccaga agcaggucga cgagcuggug caguuccgga aggcgaacag cagcaucuuc     1260

ggcaaggggu ggcacaacuu cuccgugaag cugaugaugg agcucauccc ggagcuguac     1320

gagaccagcg aggagcagau gaccauccug acgcgccucg gcaagcagaa gaccaccucc     1380

agcuccaaca agaccaagua caucgacgag aagcugcuga cggaggagau cuacaacccc     1440

gucguggcca agagcgugcg gcaggccauc aagaucguca acgccgccau caaggaguac     1500

ggggacuucg acaacaucgu gaucgagaug gcgcgcgaga ccaacgagga cgacgagaag     1560

aaggccaucc agaagaucca gaaggccaac aaggacgaga aggacgccgc caugcucaag     1620

gcggccaacc aguacaacgg caaggccgag cugccccacu ccguguucca cggccacaag     1680

cagcuggcca ccaagauccg gcuguggcac cagcaggggg agcgcugccu cuacaccggc     1740

aagacgauca gcauccacga ccugaucaac aaccccaacc aguucgaggu cgaccacauc     1800

cugccgcucu ccaucaccuu cgacgacagc cuggcgaaca aggugcuggu cuacgccacc     1860

gccaaccagg agaaggggca gcggaccccc uaccaggccc ucgacuccau ggacgacgcc     1920

uggagcuucc gcgagcugaa ggcguucgug cgggagucca agacccugag caacaagaag     1980

aaggaguacc uccugacgga ggaggacauc uccaaguucg acgugcagaa gaaguucauc     2040

gagcgcaacc uggucgacac ccgguacgcc agccgcgugg ugcucaacgc ccugcaggag     2100

cacuuccggg cccacaagau cgacaccaag gucuccgugg ugcgcggcca guucaccagc     2160

cagcugcggc gccacugggg gaucgagaag acgcgcgaca ccuaccacca ccacgccguc     2220

gacgcgcuca ucaucgccgc cuccagccag cugaaccugu ggaagaagca gaagaacacc     2280

cucgugagcu acuccgagga ccagcugcug gacaucgaga ccggcgagcu caucagcgac     2340

gacgaguaca aggaguccgu guucaaggcc cccuaccagc acuucgucga cacgcucaag     2400

agcaaggagu ucgaggacuc cauccuguuc agcuaccagg uggacuccaa guucaaccgg     2460

aagaucagcg acgccaccau cuacgcgacc cgccaggcca aggugggcaa ggacaagaag     2520

gacgagaccu acguccucgg gaagaucaag gacaucuaca cgcaggacgg cuacgacgcc     2580

uucaugaaga ucuacaagaa ggacaagucc aaguuccuga uguaccggca cgacccccag     2640

accuucgaga aggugaucga gccgauccug gagaacuacc ccaacaagga gaugaacgag     2700

aaggggaagg aggugcccug caaccccuuc cucaaguaca aggaggagca cggcuacauc     2760

cgcaaguaca gcaagaaggg caacgggccg gagaucaagu cccugaagua cuacgacagc     2820

aagcugggca accacaucga caucaccccc aagaacucca acaacaaggu cgugcuccag     2880

agcguguccc ccuggcgggc cgacgucuac uucaacaaga ccacggggaa guacgagauc     2940

cugggccuga aguacgcgga ccuccaguuc gagaagggga ccggcaccua caagaucagc     3000

caggagaagu acaacgacau caagaagaag gagggcgugg acuccgacag cgaguucaag     3060

uucacccugu acaagaacga ccugcuccug gugaaggaca cggagaccaa ggagcagcag     3120

cuguuccgcu uccucagccg gaccaugccc aagaagaagc acuacgucga gcugaagccg     3180

uacgacaagc agaaguucga gggcggggag gcccugauca aggugcucgg gaacguggcc     3240

aacuccggcc agugcaucaa ggggcuggcc aagagcaaca ucuccaucua caagguccgc     3300

accgacgugc ugggcaacca gcacaucauc aagaacgagg gcgacaagcc caagcucgac     3360

uucuga                                                                3366


<210>  1623
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1623
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucguccggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgccggg ugcgccugaa ccgccucuuc gaggaguccg gccugaucac cgacuucacc      240

aagaucagca ucaaccugaa cccguaccag cuccggguga aggggcugac cgacgagcug      300

uccaacgagg agcuguucau cgcccugaag aacaugguca agcaccgggg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccaucggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgcuaccag      480

accuacggcc agcugcgggg cgacuucacc guggagaagg acgggaagaa gcaccgccuc      540

aucaacgucu uccccacguc cgccuaccgg agcgaggcgc ugcgcauccu gcagacccag      600

caggaguuca acccccagau caccgacgag uucaucaacc gguaccucga gauccugacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggacgga cuacggccgc      720

uaccgcacca gcggggagac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuaccccg acgaguuccg ggccgccaag gccuccuaca cggcgcagga guucaaccug      840

cugaacgacc ucaacaaccu gaccgugccc accgagacca agaagcugag caaggagcag      900

aagaaccaga ucaucaacua cgugaagaac gagaaggcca ugggccccgc caagcuguuc      960

aaguacaucg ccaagcuccu guccugcgac gucgccgaca ucaaggggua ccgcaucgac     1020

aagagcggca aggcggagau ccacacguuc gaggccuacc ggaagaugaa gacccucgag     1080

acccuggaca ucgagcagau ggaccgcgag acccuggaca agcucgccua cgugcugacg     1140

cugaacaccg agcgggaggg cauccaggag gcccucgagc acgaguucgc cgacgggucc     1200

uucagccaga agcaggucga cgagcuggug caguuccgca aggcgaacag cuccaucuuc     1260

ggcaaggggu ggcacaacuu cagcgugaag cugaugaugg agcucauccc ggagcuguac     1320

gagaccuccg aggagcagau gaccauccug acgcggcucg gcaagcagaa gaccaccagc     1380

uccagcaaca agaccaagua caucgacgag aagcugcuga cggaggagau cuacaacccc     1440

guggucgcca aguccgugcg ccaggccauc aagaucguga acgccgccau caaggaguac     1500

ggggacuucg acaacaucgu caucgagaug gcgcgggaga ccaacgagga cgacgagaag     1560

aaggccaucc agaagaucca gaaggccaac aaggacgaga aggacgccgc caugcucaag     1620

gcggccaacc aguacaacgg caaggccgag cugccccaca gcguguucca cggccacaag     1680

cagcuggcca ccaagauccg ccuguggcac cagcaggggg agcggugccu cuacaccggc     1740

aagacgaucu ccauccacga ccugaucaac aacagcaacc aguucgaggu ggaccacauc     1800

cugccccucu ccaucaccuu cgacgacagc cuggcgaaca agguccuggu guacgccacc     1860

gccaaccagg agaaggggca gcgcaccccg uaccaggccc ucgacuccau ggacgacgcc     1920

uggagcuucc gggagcugaa ggcguucguc cgcgagucca agacccugag caacaagaag     1980

aaggaguacc uccugacgga ggaggacauc uccaaguucg acgugcggaa gaaguucauc     2040

gagcgcaacc ugguggacac ccgguacgcc agccgcgucg ugcucaacgc ccugcaggag     2100

cacuuccggg cccacaagau cgacaccaag guguccgucg ugcgcggcca guucaccagc     2160

cagcugcgcc ggcacugggg gaucgagaag acgcgcgaca ccuaccacca ccacgccgug     2220

gacgcgcuca ucaucgccgc cagcucccag cugaaccugu ggaagaagca gaagaacacc     2280

cucgucagcu acuccgagga ccagcugcug gacaucgaga ccggcgagcu caucagcgac     2340

gacgaguaca aggaguccgu guucaaggcc cccuaccagc acuucgugga cacgcucaag     2400

agcaaggagu ucgaggacuc cauccuguuc agcuaccagg ucgacuccaa guucaaccgg     2460

aagaucagcg acgccaccau cuacgcgacc cgccaggcca aggugggcaa ggacaaggcc     2520

gacgagaccu acgugcucgg gaagaucaag gacaucuaca cgcaggacgg cuacgacgcc     2580

uucaugaaga ucuacaagaa ggacaagucc aaguuccuga uguaccggca cgacccccag     2640

accuucgaga aggucaucga gcccauccug gagaacuacc cgaacaagca gaucaacgag     2700

aaggggaagg aggugcccug caaccccuuc cucaaguaca aggaggagca cggcuacauc     2760

cgcaaguaca gcaagaaggg caacgggccc gagaucaagu cccugaagua cuacgacagc     2820

aagcugggca accacaucga caucaccccg aaggacucca acaacaaggu gguccuccag     2880

agcguguccc ccuggcgggc ggacguguac uucaacaaga ccacggggaa guacgagauc     2940

cugggccuga aguacgccga ccuccaguuc gagaagggga ccggcaccua caagaucagc     3000

caggagaagu acaacgacau caagaagaag gagggcgucg acagcgacuc cgaguucaag     3060

uucacccugu acaagaacga ccugcuccug gugaaggaca cggagaccaa ggagcagcag     3120

cuguuccgcu uccucagccg gaccaugccc aagcagaagc acuacgugga gcugaagccc     3180

uacgacaagc agaaguucga gggcggggag gcccugauca agguccucgg gaacguggcc     3240

aacuccggcc agugcaagaa ggggcugggc aagagcaaca ucuccaucua caaggugcgc     3300

accgacgucc ugggcaacca gcacaucauc aagaacgagg gggacaagcc gaagcucgac     3360

uucuga                                                                3366


<210>  1624
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1624
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucguccggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgccggg ugcgccugaa ccgccucuuc gaggaguccg gccugaucac cgacuucacc      240

aagaucagca ucaaccugaa cccguaccag cuccggguga aggggcugac cgacgagcug      300

uccaacgagg agcuguucau cgcccugaag aacaugguca agcaccgggg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgcuaccag      480

accuacggcc agcugcgggg cgacuucacc gucgagaagg acgggaagaa gcaccgccuc      540

aucaacgugu uccccacguc cgccuaccgg agcgaggcgc ugcgcauccu gcagacccag      600

caggaguuca acccccagau caccgacgag uucgugaacc gguaccucga gauccugacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggacgga cuacggccgc      720

uaccgcacca acggggagac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuaccccg aggaguuccg ggccgccaag gccagcuaca cggcgcagga guucaaccug      840

cugaacgacc ucaacaaccu gaccgucccc accgagacca agaagcuguc caaggagcag      900

aagaaccaga ucaucaacua cgugaagaac gagaaggcca ugggccccgc caagcuguuc      960

aaguacaucg ccaagcuccu gagcugcgac guggccgaca ucaaggggua ccgcaucgac     1020

aaguccggca aggcggagau ccacacguuc gaggccuacc ggaagaugaa gacccucgag     1080

acccuggaca ucgagcagau ggaccgcgag acccuggaca agcucgccua cguccugacg     1140

cugaacaccg agcgggaggg cauccaggag gcccucgagc acgaguucgc cgacgggagc     1200

uucucccaga agcaggucga cgagcuggug caguuccgca aggcgaacag cagcaucuuc     1260

ggcaaggggu ggcacaacuu cuccgugaag cugaugaugg agcucauccc ggagcuguac     1320

gagaccagcg aggagcagau gaccauccug aagcggcucg gcaagcagaa gacgaccucc     1380

agcuccaaca agaccaagua caucgacgag aagcugcuga ccgaggagau cuacaacccc     1440

gugguggcca agagcauccg ccaggccauc aagaucguca acgccgccau caaggaguac     1500

ggggacuucg acaacaucgu gaucgagaug gcgcgggaga cgaacgagga cgacgagaag     1560

aaggccaucc agaagaucca gaaggccaac aaggacgaga aggacgccgc caugcucaag     1620

gcggccaacc aguacaacgg caaggccgag cugccccacu ccguguucca cggccacaag     1680

cagcuggcca ccaagauccg ccuguggcac cagcaggggg agcggugccu cuacaccggc     1740

aagaccauca gcauccacga ccugaucaac aaccccaacc aguucgaggu cgaccacauc     1800

cugccgcucu ccaucacguu cgacgacagc cuggcgaaca aggugcuggu cuacgccacc     1860

gccaaccagg agaaggggca gcgcaccccc uaccaggccc ucgacuccau ggacgacgcc     1920

uggagcuucc gggagcugaa ggcguucgug cgcgagucca agacccugag caacaagaag     1980

aaggaguacc uccugaccga ggaggacauc uccaaguucg acgugcggaa gaaguucauc     2040

gagcgcaacc uggucgacac gcgguacgcc agccgcgugg ugcucaacgc ccugcaggag     2100

cacuuccggg cccacaagau cgacaccaag gucuccgugg ugcgcggcca guucaccagc     2160

cagcugcgcc ggcacugggg gaucgagaag acccgcgaca cguaccacca ccacgccguc     2220

gacgcgcuca ucaucgccgc cuccagccag cugaaccugu ggaagaagca gaagaacacc     2280

cucgugagcu acuccgagga ccagcugcug gacaucgaga ccggcgagcu caucagcgac     2340

gacgaguaca aggaguccgu guucaaggcc cccuaccagc acuucgucga cacccucaag     2400

agcaaggagu ucgaggacuc cauccuguuc agcuaccagg uggacuccaa guucaaccgg     2460

aagaucagcg acgccacgau cuacgcgacc cgccaggcca aggugggcaa ggacaagaag     2520

gacgagaccu acguccucgg gaagaucaag gacaucuaca cccaggacgg cuacgacgcc     2580

uucaugaaga ucuacaagaa ggacaagucc aaguuccuga uguaccggca cgacccccag     2640

acguucgaga aggugaucga gccgauccug gagaacuacc ccaacaagga gaugaacgag     2700

aaggggaagg aggugcccug caaccccuuc cucaaguaca aggaggagca cggcuacauc     2760

cgcaaguaca gcaagaaggg caacgacccg gagaucaagu cccugaagua cuacgacagc     2820

aagcugggga accacaucga caucaccccc aaggacucca acaacaaggu cgugcuccag     2880

agcguguccc ccuggcgggc cgacgucuac uucaacaaga ccaccggcaa guacgagauc     2940

cuggggcuga aguacgcgga ccuccaguuc gagaagggca cggggaccua caagaucagc     3000

caggagaagu acaacgacau caagaagaag gagggcgugg acuccgacag cgaguucaag     3060

uucacccugu acaagaacga ccugcuccug gugaaggaca ccgagacgaa ggagcagcag     3120

cuguuccgcu uccucagccg gaccaugccc aagcagaagc acuacgucga gcugaagccg     3180

uacgacaagc agaaguucga gggcggggag gcccugauca aggugcucgg caacguggcc     3240

aacuccgggc agugcaagaa gggccugggc aagagcaaca ucuccaucua caagguccgc     3300

accgacgugc uggggaacca gcacaucauc aagaacgagg gcgacaagcc caagcucgac     3360

uucuga                                                                3366


<210>  1625
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1625
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaagaacu ccgggaagau cauccacgcc aacagccgcc ucuuccccgc ggccaccgcc      120

gacaacaacg uggagcggcg caagaaccgg caggcccgcc ggcugcaccg ccggaagaag      180

caccgcggcg uccgguucca ggaccuguuc gaggacuacg gccuccugac cgacuucucc      240

aaggugagca ucaaccugaa cccguaccgc cuccgggugg acgggcugaa ccagcagcug      300

acgaacgagg agcuguucau cgcccucaag aacaucguca agcgccgggg caucuccuac      360

cuggacgacg cgagcgagga cgggggcacc guguccagcg acuacgggaa ggccguggag      420

gagaaccgca agcuccuggc cgagcagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc agguccgcgg ggacuucacc gugguggaga acggcgagaa gcggcgccuc      540

aucaacgucu ucuccacgag cgccuaccgg aaggaggcgg agcgcauccu gcggaagcag      600

caggaguuca acuccaagau caccgacgag uucaucgagg acugccugaa gauccucacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccgcaccga cuacgggcgg      720

uuccgcacgg acggcaccac ccuggacaac aucuucggga uccugaucgg caagugcacc      780

uucuaccccg acgaguaccg ggccagcaag gccagcuaca cggcccagga guucaaccuc      840

cugaacgacc ugaacaaccu caccgugccg accgagacca agaagcuguc cgaggagcag      900

aagaagguga ucgucgagua cgccaaggag gcgaagacgc ugggggccag cacccuccug      960

aaguacaucg ccaagaugau cgacgccucc guggaccaga uccgcggcua ccggguggac     1020

gucaacaaca agcccgagau ccacaccuuc gaggccuacc gcaagaugca gagccuggag     1080

accaucuccg ugggcgagcu cagccggaac gugcuggacg agcuggcgca cauccucacg     1140

cugaacaccg agcgcgaggg gaucgaggag gccaucaaca ccaagcugaa ggacagcuuc     1200

ucccaggacc agguccucga gcuggugcag uuccggaaga acaacagcuc ccuguucagc     1260

aagggcuggc acaacuucuc ccucaagcug augauggagc ugauccccga gcucuacgag     1320

accagcgagg agcagaugac gauccugacc cgccugggga agcagaaguc caaggagauc     1380

agcaagcgga ccaaguacau cgacgagaag gagcucaccg aggagaucua caaccccgug     1440

gucgccaagu ccgugcgcca ggccaucaag aucaucaacg aggccacgaa gaagcacggc     1500

aucuucgaca acaucgucau cgagauggcg cgggagaaca acgaggagga cgccaagaag     1560

gacuacauca agcgccagaa ggccaaccag gacgagaaga acgccgccau ggagaaggcg     1620

gccuuccagu acaacgggaa gaaggagcug ccggacagca ucuuccacgg ccagaaggag     1680

cuggccacca agauccggcu guggcaccag cagggcgaga agugccucua caccgggaag     1740

aacaucccca ucuccgaccu gauccacaac caguacaagu acgagaucga ccacauccug     1800

ccccucagcc uguccuucga cgacagccug uccaacaagg ugcucgugcu ggccaccgcg     1860

aaccaggaga agggccagcg cacgcccuuc caggcccugg acagcaugga cgacgccugg     1920

uccuaccggg aguucaagag cuacgucaag gaguccaagc uccuggggaa caagaagaag     1980

gaguaccugc ucaccgagga ggacaucagc aagaucgagg ugaagcagaa guucaucgag     2040

cgcaaccuga ucgacacccg guacagcucc cgcguggucc ugaacgcccu ccaggacuuc     2100

uacaaggccc acaaguucga caccacgauc agcguggugc gcggccaguu caccucccag     2160

cugcggcgca agugggggcu ggagaagucc cgggagaccu accaccacca cgcggucgac     2220

gcccucauca ucgccgccag cagccagcug cgccugugga agaagcagaa caacccgcuc     2280

aucgccuaca aggagggcca guucguggac uccgagaccg gcgagaucau cagccugucc     2340

gacgacgagu acaaggagcu gguguucaag gcgcccuacg accacuucgu cgacacccuc     2400

agcuccaaga cguucgagga cagcauccug uucuccuacc agguggacag caaguucaac     2460

cggaagaucu ccgacgccac caucuacgcc acccgcaagg ccaagcugga caaggagaag     2520

uccgaggaga ccuacgugcu cgggaagauc aaggacaucu acagccaggc cggcuacgac     2580

gcguucauca agaucuacaa caaggacaag agcaaguucc ugauguacca caaggacccc     2640

cagacguucg agaaggucau cgaggagauc cugcggaccu accccagcaa ggagcucaac     2700

gacaagaaca aggagauccc gugcaacccc uucgagaagu accgccagga gaacgggccc     2760

auccggaagu acuccaagaa guccaacggc cccgagauca agagccugaa guacuacgac     2820

aacaagcugg gcaaccacau cgacaucacc ccggagagcu ccgacaacca gguggugcuc     2880

cagagccuga agcccuggcg caccgacguc uacuucaacc accagacgaa gaaguacgag     2940

cugauggggc ucaaguacuc cgaccugagc uucgagaagg gcuccgggaa guacagcauc     3000

uccaaccaga aguacaacag caucaagcgg aucgagggca ucgacgagca guccgaguuc     3060

aaguucaccc uguacaagaa cgaccucauc cugaucaagg acagcgagaa caacgagcag     3120

aagcuguucc gcuucaacuc ccggaacgac accgccaagc acuacaucga gcucaagccc     3180

uacgacaagg ccaaguucga cggggagcag gagcugauca ccauccuggg caacguggcc     3240

aagggcgggc gcugccucaa gggccugaac aagagcaaca ucuccaucuu caaggugaag     3300

acggacgucc uggggaagaa gcacaucauc aagaaggagg gcgacgagcc caagcucaag     3360

uucuga                                                                3366


<210>  1626
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1626
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

aacaaggaca ccggggagau cauccacacc aacucccgca ucuuccccgc cgcgacggcc      120

gacaacaacg uggagcggcg caacagccgg caggaccgcc ggcucaaccg ccggaagaag      180

caccgcggcg uccggcugca cgaccuguuc gaggacuacg accuccugac cgacuucucc      240

aaggugagca ucaaccugaa cccguaccgc cuccgggugc agggccugga caacucccug      300

accaccgagg agcuguucau cgcccucaag aacaucguca agcgccgggg gaucagcuac      360

cuggacgacg ccuccgagga cggcggggug gugagcuccg acuacggcaa ggccgucgag      420

gagaaccgca agcuccuggc ggagcagacg cccgggcaga uccagcugga gcgguucaag      480

aaguacggcc aggugcgcgg cgacuucacc guggucgaga acggggagaa gcggcgccuc      540

aucaacgugu ucagcaccuc cgccuaccgg aaggaggccg agcgcauccu gcggaagcag      600

caggaguuca acaaccagau caccgacaag uucaucgagg acuaccugaa gauccucacg      660

gagaagcgca aguacuacca cggccccggg aacgagaagu cccgcaccga cuacggccgg      720

uuccgcaccg acggcaccac gcuggacaac aucuucggga uccugaucgg caagugcacc      780

uucuacccca acgaguaccg ggccagcaag gcgagcuaca ccgcccagga guucaaccuc      840

cugaacgacc ugaacaaccu caccgugccg acggagacca agaagcuguc cgaggagcag      900

aagaagacca ucgucgagua cgccaagagc gccaagaccc ugggggccuc cacgcuccug      960

aaguacaucg cgaagaugau cgacgccagc guggaccaga uccgcggcua ccggguggac     1020

gucaacaaca agcccgagau gcacaccuuc gagguguacc gcaagaugca gucccuggag     1080

accaucagcg ugugggagcu cagccggaac auccuggacg agcuggccca cauccucacc     1140

cugaacacgg agcgcgaggg gaucgaggag gccaucaaca ccaagcugau ggacgucuuc     1200

ucccaggacc aggugcucga gcuggugcag uuccggaaga acaacagcuc ccuguucagc     1260

aagggcuggc acaacuucuc ccgcaagcuc augauggagc ugauccccga gcuguacgag     1320

accagcgagg agcagaugac cauccucacg cggcugggca agcagaaguc caaggagacc     1380

agcaagcgca ccaaguacau cgacgagaag gaggucaccg aggagaucua caaccccgug     1440

gucgccaagu ccgugcggca ggcgaucaag aucaucaacg aggccacgaa gaaguacggg     1500

aucuucgaca acaucgugau cgagauggcc cgcgagaaca acgaggagga cgccaagaag     1560

gacuacauca agcggcagaa ggccaaccag gacgagaaga acgucgcgau cgagaaggcc     1620

gccuuccagu acaacggcaa gaaggagcug ccggacagca ucuuccacgg gcacaaggag     1680

cucgccacca agauccgccu guggcaccag cagggcgaga agugccugua caccgggaag     1740

aacaucccca ucuccgaccu gauccacaac caguacaagu acgagaucga ccacauccuc     1800

ccccugagcc uguccuucga cgacagccuc uccaacaagg ugcuggugcu ggccaccgcg     1860

aaccaggaga agggccagcg gacgcccuuc caggcccucg acagcaugga cgacgccugg     1920

uccuaccgcg aguucaagag cuacgucaag gacuccaagc ugcugggcaa caagaagaag     1980

gacuaccucc ugaccgagga ggacaucagc aagaucgagg ugaagcagaa guucaucgag     2040

cggaaccugg uggacacccg cuacagcucc cgcgucgugc ucaacacccu gcaggacuuc     2100

uacaaggccc acaaguucga caccacgauc agcguggucc gggggcaguu caccucccag     2160

cugcgccgga aguggggccu cgagaagucc cgcgagaccu accaccacca cgcgguggac     2220

gcccugauca ucgccgccag cagccagcug cggcucugga agaagcaggc caacccgcug     2280

aucuccuaca aggaggacca guucguggac agcgagaccg gggagaucau cucccugacg     2340

gacgacaagu acaaggagcu cgucuucaag gcgcccuacg cccacuucgu ggacacccug     2400

agcuccaaga aguucgagga cagcauccug uucuccuacc agguggacag caaguacaac     2460

cgcaagaucu ccgacgccac caucuacgcc acccggaagg ucaagcucga caaggacaag     2520

agcgaggaga cguacgugcu gggcaagauc aaggacaucu acucccaggc cggguacgac     2580

gcguucauca agaucuacaa caaggacaag agcaaguucc ugauguacca caaggacccc     2640

cagaccuucg agaaggugau cgaggagauc cuccgcaucu accccagcaa ggaguucaac     2700

gagaagaaca aggagguccc gugcaacccc uucgagaagu accggcagga gaacggcccc     2760

aucugcaagu acuccaagaa gggcaacggg cccgagauca agagccugaa guacuacgac     2820

aagaacaucc cggaguccuu caucgacauc acccccagca acuccaagaa caaggugauc     2880

cugcgccagc ucaaccccug gcggaccgac guguacuuca accaccagac ggagaaguac     2940

gagcugcugg gccucaagua cgccgaccug caguucgaga agaagaccga gaccuacagc     3000

aucaccaucg acaaguacaa cgagaucaag gagaaggagg gggucggcga gcacuccgag     3060

uucaaguuca cgcuguacaa gaacgaccuc cugcugauca aggacaugga gaucaaggag     3120

gagcagaucu uccgcuuccu cagccggacc augcccaaca agaagcacua cguggagcug     3180

aagccguacu acaagggcaa cuucgagggg aaggagcgca ucaagguguu caacaccacc     3240

uccaacggcc ggugccugaa ggggcucggc aagaaggggc ugagcaucua caagaucaag     3300

acggacgucc ugggcaacaa gcacuucauc aagaaggagg gcgagaagcc caagcucaag     3360

uucuga                                                                3366


<210>  1627
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1627
auggaccgca ccgaggugaa caucggcuuc gacaucggga ucagcuccgu cggcuggagc       60

gugguggcca ccaaguccgg gcggauccug gagagcggcg ucucccucuu cagcggggcc      120

uccgcggcca agaacgagga gcgccggaac uuccgccaga gccggcgccu gcugcggcgc      180

cggaagaacc gcaucuccga ccucaagaag uaccugaagg agcagagcuu cacguacaac      240

ggcgagcugc ucaacgagaa ccccuacgag auccggguga agggccugac cgagaagcug      300

uccaaggagg agaucgcccu cgcccugcug cacaucguga agcgccgcgg ggucagcuac      360

gaccucggcg acaucgagga ggaggggacc accggcuccu acaaggaggg ggugaagaag      420

aaccagaagc ugcugaagga gaagacgccg gccgagaucc aguuccagcg gcucaagaag      480

uuccacaagg ugcggggcca gaucgcgauc gacgagaacg acguccugcu gaacguguuc      540

cccaaccacg ccuacgugga cgaggcccag cagauccucu acacccagca ggaguucuac      600

cccgagauca acgagaccuu caucaaccag gucaccgacu ucaucagccg caagcgggac      660

uacuucaucg gccccggguc cgagaagucc cgcacggacu acggcaucua ccggaccgac      720

gggaccaccc ugcagaaccu guucagcauc cucaucggca aggacaagau cuucccggac      780

gaguuccgcg ccgcgggcaa cagcuacacg gcccaggugu acaaccugcu gaacgaccuc      840

aacaaccugg ccaucgacga caccgagacc gggaagcuga ccaagcagca gaaggagaag      900

aucaucaugg agcucaagac gaccgagaag uccgugaaca ugaugaagcu gaucgccaag      960

accgccggcg ucaccgugga gcagaucagc cgguaccgca ucaaccgcga cggcaagagc     1020

gagauccaca acauggcggu guaccggaag auccgcaagg ucuuccugga gcucggguac     1080

gagaucaacg aguggcccuc ccagcugcug gacgagcucg gggacgcccu gagccugaac     1140

acggagaacg gcgagauccg gcgcauccuc accgacaacc uggccaacaa guacaagauc     1200

cuggaccagc agcucaucaa ccugaucauc gagaacaaga acgccuucac ccuguccacc     1260

aaccagaagu ggcaccgguu cagcuacaag acgaugaaga uccucauccc cgagcugcug     1320

gagaccaaca aggagcagau gaccauccuc accgagaugg gccugaugaa gaaggaccag     1380

cgcaacuacg uggggaagaa caagaucgac uccgaggccc ugcucgagaa ccuguacaac     1440

cccguggucc gcaagagcac gcggcagacc augaagcugu ucaacacccu gcucaagaag     1500

uacccgaaca ucgcguacgu ggugaucgag augccccggg aggacgacga ggacaaggcc     1560

cgccgggacu acgucaaguu ccagaaggac aacgagaagg agaaggacgc cgcccugaag     1620

aacuucauca uggaguccgg caagagcgag gccgagguga ucgcgaagua ccgccagucc     1680

aagaaguucc ggagcaaggu ccgccugugg uaccagcaga uggggcggug ccucuacucc     1740

ggcaaggcca ugcccgccga gcgcguguuc cgggacgacc acuucaccga caucgaccac     1800

gugauccccc ugagcgucuc cuucgacgac gggaugaaca acaaggugcu gugccucgcc     1860

gagaugaacc gccagaagga caagcagacg ccguacgccu ucuucaacgc gggccacggc     1920

cagggguucc aggccaugaa cgccaugcug aagaacaaca accggaucag cuccgccaag     1980

aagcgcaacc uccuguucac cgagaaccug aacgaccugg aggugcgcaa gcgguucauc     2040

gagcgcaacc ucgucgacac ccgguacgcg agccgcgugg ugcugaacga gcugcagcag     2100

uucgucaagg ccaagcagcu cgacaccaag gugacgguga uccggggcaa guucaccucc     2160

aagcugcgcg agaaguggaa caucaacaag agccgggaca cccaccacca ccacgccguc     2220

gacgccgccg ugaucgcggu gucccccaug cugaaccucu gggagaaggg ggccagcauc     2280

auccccaaca aggucggcga gaacgugauc gacaucaaga ccggggagau caucaccgac     2340

cacgacuacg aggccgacgu guacgagcug cccuacgcca aguuccugga gcagaucccg     2400

aaggucaaga aggacaucaa guuccgccac cagguggaca agaagaagaa ccggaaggug     2460

agcgacgcca cgaucuacac cacccgcaag gcgaaggucg gcaaggacaa ggaggagacc     2520

gacuacgugc ucggcacgau caaggacauc uacucccugg agggguacgu gagcuucaag     2580

aagaucuaca acaaggacaa ggccaaguuc cugauggccc ggaucgaccc caagaccuuc     2640

gagaagcucg agaagaucgu cgccgaguac cccgagucca ucgagguggu gcaggacaac     2700

ggcaaggucg acgugcagaa gaucagcccc uucgagcugu accgcaagga caacggguuc     2760

gugaccaagu ucgccaagaa gaacaacggc ccggcgguca agucccugaa guacuacgac     2820

agcaagcucg gcuccagcau cgacgugacc cccgacaacg ccaagaacaa gaaggugauc     2880

cugcaguccc ugaagcccug gcggacggac gucuacuuca acgaggagaa ccaggaguac     2940

gagaucaugg ggcucaagua cagcgaccug aaguucggca agaacgggaa guacggcgug     3000

cccaaggugc gcuacgagga gaucaagcag gaggaggaga ucuccgaggg gagcaaguuc     3060

cuguucuccc ucuaccgggg cgaccgcauc aaggucacca acgagcuggg cgagaaccug     3120

gaccuccugu ucagcucccg gacccugccg gcccagaagg gguacgugga gcucaagccc     3180

aucgacaaga ccaaguucga cggcaaggag aaggugagcu ucuacgggac ggucaccccc     3240

aacggccagu ucgugaagaa guucgccaag aagggguaca agauccugaa gcugaacacc     3300

gacgagcucg gcaacaccua caucgagaac ggcgagaagg agcccuccga cauccuggac     3360

auguga                                                                3366


<210>  1628
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1628
augaacggcc uggugcucgg gcuggacauc ggcaucggga gcgucggcgu ggggauccug       60

aacaaggaca ccggcgagau cauccacgcc aacucccgca ucuuccccgc cgcgaccgcc      120

gacaacaacg ugguccggcg cagcucccgg cagggccgcc ggcucaacca ccgcaagaag      180

caccggagcg ugcgccugaa ggaccuguuc gaguacuacg accuccugac ggacuucucc      240

aaggugagca ucaaccugaa cccguaccgg cuccgcguca acgggcugaa ccagcagcug      300

accaacgagg agcuguucau cgcccucaag aacaucguga agcggcgcgg caucuccuac      360

cuggacgacg ccagcgagga cgggggcgcc guguccagcg acuacgggaa ggcggucgag      420

gagaaccgga agcuccuggc cgagcagacc cccggccaga uccagcugga gcgcuucgag      480

aaguacggcc aggugcgcgg ggacuucacc guggucgaga acggcgagaa gcggcggcuc      540

aucaacgugu ucuccacgag cgccuacaag aaggaggccg agcgcauccu gcggaagcag      600

caggaguaca acaaccagau cauggacgag uucaucgcgg acuaccugac cauccucacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccggaccga cuacgggcgc      720

uuccgcacgg acggcaccac ccuggacaac aucuucggga uccugaucgg caagugcacc      780

uucuaccccg agaaguaccg ggccagcaag gccuccuaca cggcccagga guucaaccuc      840

cugaacgacc ugaacaaccu caccgugccg accgagacca agaagcugag cgaggagcag      900

aagaagacga ucgucgagua cgccaagucc gcgaagaccc ugggggccag cacccuccug      960

aaguacaucg ccaagaugau cgacgccucc guggaccaga uccgcggcua ccggguggac     1020

gucaagaaca agcccgagau gcacaccuuc gagguguacc gcaagaugca gagccuggag     1080

acgaucagcg ugggcgaguu cucccgggag guccucgacg agcuggccca cgugcugacc     1140

cucaacaccg agcgcgaggg gaucgaggag gcgaucaaca ccaagcugcg ggacagcuuc     1200

ucccaggacc agauccugga gcucgugcag uuccgcaaga acaacagcuc ccuguucagc     1260

aagggcuggc acaacuucuc ccugaagcuc augauggagc ugauccccga gcuguacgag     1320

acgagcgagg agcagauggc cauccucacc cggcugggga agcagaaguu caaggagacc     1380

uccaagcgca ccaaguacau cgacgagcag gaggucacgg aggagaucua caaccccgug     1440

gucgccaaga gcgugcggca ggccaucaag aucaucaacg aggccaccaa gaaguacggc     1500

aucuucgaca acaucgugau cgagauggcg cgcgagaaca acgaggagga ggccaagaag     1560

gacuacauca agcggcagaa ggccaaccac gacgagaaga acgccgccau ggagaaggcg     1620

gccuuccagu acaacgggga gaaggagcug ccggacucca ucuuccacgg ccacaaggag     1680

cucgccacca agguccgccu guggcaccag cagggcgaga agugccugua caccgggaag     1740

aacaucccca ucagcgaccu gauccacaac caguacaagu acgagaucga ccacauccuc     1800

ccccuguccc ugagcuucga cgacucccuc agcaacaagg ugcuggugcu ggccacggcg     1860

aaccaggaga agggccagcg gacccccuuc caggcccucg acuccaugga cgacgccugg     1920

agcuaccgcg aguucaaguc cuacgucaag gacagcaaga gccuggggaa caagaagaag     1980

gacuaccugc ucaccgagga ggacaucucc aagaucgagg ugaagcagaa guucaucgag     2040

cggaaccugg uggacacccg cuacagcucc cgggucgugc ugaacacgcu ccaggacuuc     2100

uacaaggccc acaaguucga caccaccauc agcguggucc gcggccaguu caccucccag     2160

cugcgccgga agugggggcu ggagaagucc cgcgagaccu accaccacca cgccguggac     2220

gcgcucauca ucgccgccag cagccagcug cggcugugga agaagcagaa caacccgcuc     2280

aucuccuaca aggagggcca guucguggac agcgagacgg gcgagaucau cucccugacc     2340

gacgacgagu acaaggagcu ggucuucaag gcccccuacg accacuucgu ggacacccuc     2400

cgcagcaaga aguucgagga cuccauccug uucagcuacc agguggacuc caaguucaac     2460

cggaagauca gcgacgccac caucuacgcg acgcgcaaga ccaagcugga caaggacaag     2520

uccgaggaga ccuacguccu cgggaagauc aaggacaucu acagccagag cggcuacgac     2580

gccuucauca agaucuacaa caaggacaag uccaaguucc ugauguacca caaggacccc     2640

cagaccuucg agaaggugau cgaggagauc cugcggacgu accccagcaa ggaguucaac     2700

gagaagaaca aggagguguc cugcaacccg uucgagaagu acaagcagga gaacgggccc     2760

auccgcaagu acagcaagaa gggcaacggc cccgagauca agucccucaa guacuacgac     2820

aagaacaucc ccgagagcuu caucaacauc accccgucca acagcaagaa caaggucauc     2880

cugcggcagc ugaaccccug gcgcaccgac guguacuuca accaccagac cgagaaguac     2940

gagcuccugg ggcugaagua cgccgaccuc caguucgaga agaagacggg caccuacucc     3000

aucaccaucg acaaguacaa cgagaucaag gagaaggagg gggugagcga gcacuccgag     3060

uucaaguuca cccuguacaa gaacgaccug cuccugauca aggacacgga gaucaaggag     3120

gagcagaucu uccgguuccu gagccgcacc augcccaacc agaagcacua cgucgagcuc     3180

aagcccuacu acaagggcaa cuucgagggg aaggagcgga ucaagguguu caacaccacc     3240

uccaacggcc gcugccugaa gggccugcgg aagaaggggc ucagcaucua caagaucaag     3300

acggacgugc ugggcaacaa gcacuucauc aagaaggagg gggagaagcc gaagcugaag     3360

uucuga                                                                3366


<210>  1629
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1629
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucguccggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgcaucg ugcggcugaa ccgccucuuc gaggaguccg gccugaucac cgacuucacc      240

aagaucagca ucaaccugaa cccguaccag cuccggguga aggggcugac cgacgagcug      300

uccaacgagg agcuguucau cgcccucaag aacaugguca agcaccgcgg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgguaccag      480

accuacggcc agcugcgcgg cgacuucacc gucgagaagg acgggaagaa gcaccggcuc      540

aucaacgugu uccccacguc cgccuaccgc agcgaggcgc ugcggauccu gcagacccag      600

caggaguuca acccccagau caccgacgag uucaucaacc gcuaccucga gauccugacc      660

ggcaagcgga aguacuacca cgggccgggc aacgagaagu cccgcacgga cuacggccgg      720

uaccgcacca gcggggagac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuaccccg acgaguuccg cgccgccaag gccuccuaca cggcgcagga guucaaccug      840

cugaacgacc ucaacaaccu gaccgugccc accgagacca agaagcugag caaggagcag      900

aagaaccaga ucaucaacua cgucaagaac gagaaggcca ugggccccgc caagcuguuc      960

aaguacaucg ccaagcuccu guccugcgac guggccgaca ucaaggagua ccggaucgac     1020

aagagcggga aggcggagau ccacacguuc gaggccuacc gcaagaugaa gacccucgag     1080

acccuggaca ucgagcagau ggaccgggag acccuggaca agcucgccua cgugcugacg     1140

cugaacaccg agcgcgaggg cauccaggag gcccucgagc acgaguucgc cgacggcucc     1200

uucagccaga agcaggucga cgagcuggug caguuccgga aggcgaacag cuccaucuuc     1260

gggaagggcu ggcacaacuu cagcgugaag cugaugaugg agcucauccc ggagcuguac     1320

gagaccuccg aggagcagau gaccauccug acgcgccucg ggaagcagaa gaccaccagc     1380

uccagcaaca agaccaagua caucgacgag aagcugcuga cggaggagau cuacaacccc     1440

gucguggcca aguccgugcg gcaggccauc aagaucguca acgccgccau caaggaguac     1500

ggcgacuucg acaacaucgu gaucgagaug gcgcgcgaga ccaacgagga cgacgagaag     1560

aaggccaucc agaagaucca gaaggccaac aaggacgaga aggacgccgc caugcucaag     1620

gcggccaacc aguacaacgg gaaggccgag cugccccaca gcguguucca cggccacaag     1680

cagcuggcca ccaagauccg gcuguggcac cagcagggcg agcgcugccu cuacaccggg     1740

aagacgaucu ccauccacga ccugaucaac aacagcaacc aguucgaggu cgaccacauc     1800

cugccccucu ccaucaccuu cgacgacagc cuggcgaaca aggugcuggu cuacgccacc     1860

gccaaccagg agaagggcca gcggaccccg uaccaggccc ucgacuccau ggacgacgcc     1920

uggagcuucc gcgagcugaa ggcguucgug cgggagucca agacccugag caacaagaag     1980

aaggaguacc uccugacgga ggaggacauc uccaaguucg acgugcgcaa gaaguucauc     2040

gagcggaacc uggucgacac ccgcuacgcc agccgggugg ugcucaacgc ccugcaggag     2100

cacuuccgcg cccacaagau cgacaccaag gucuccgugg ugcgggggca guucaccagc     2160

cagcugcgcc gccacugggg caucgagaag acgcgggaca ccuaccacca ccacgccguc     2220

gacgcgcuca ucaucgccgc cagcauccag cugaaccugu ggaagaagca gaagaacacc     2280

cucguguccu acagcgagga ccagcugcug gacaucgaga ccggggagcu caucuccgac     2340

gacgaguaca aggagagcgu guucaaggcc cccuaccagc acuucgucga cacgcugaag     2400

uccaaggagu ucgaggacag cauccuguuc uccuaccagg uggacagcaa guucaaccgc     2460

aagaucuccg acgccaccau cuacgcgacc cggcaggcca aggugggcaa ggacaaggcc     2520

gacgagaccu acguccucgg caagaucaag gacaucuaca cgcaggacgg guacgacgcc     2580

uucaugaaga ucuacaagaa ggacaagagc aaguuccuga uguaccgcca cgacccccag     2640

accuucgaga aggugaucga gcccauccug gagaacuacc cgaacaagca gaucaacgag     2700

aagggcaagg aggugcccgu caaccccuuc cucaaguaca aggaggagca cggguacauc     2760

cggaaguacu ccaagaaggg caacggcccc gagaucaaga gccugaagua cuacgacucc     2820

aagcugggga accacaucga caucaccccg aaggacagca acaacaaggu ggugcuccag     2880

uccgucagcc ccuggcgcgc ggacguguac uucaacaaga ccacgggcaa guacgagauc     2940

cuggggcuga aguacgccga ccuccaguuc gagaagggca ccgggaccua caagaucucc     3000

caggagaagu acaacgacau caagaagaag gagggcgugg acagcgacag cgaguucaag     3060

uucacccugu acaagaacga ccugcuccug gucaaggaca cggagaccaa ggagcagcag     3120

cuguuccggu uccucucccg caccaugccc aagcagaagc acuacgugga gcugaagccc     3180

uacgacaagc agaaguucga gggcggggag gcccugauca aggugcucgg caacgucgcc     3240

aacagcgggc agugcaagaa gggccugggg aaguccaaca ucagcaucua caaggugcgg     3300

accgacgugc ugggcaacca gcacaucauc aagaacgagg gcgacaagcc gaagcucgac     3360

uucuga                                                                3366


<210>  1630
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1630
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaagaacu ccgggaagau cauccacgcc aacagccgcc ucuuccccgc ggccaccgcc      120

gacaacaacg uggagcggcg caagaaccgg caggcccgcc ggcugaaccg ccggaagaag      180

caccgcggcg uccgguucca ggaccuguuc gaggacuacg gccuccugac cgacuucucc      240

aaggugagca ucaaccugaa cccguaccgc cuccgggugg acgggcugaa ccagcagcug      300

acgaacgagg agcuguucau cgcccucaag aacaucguca agcgccgggg caucuccuac      360

cuggacgacg cgagcgagga cgggggcgcc guguccagcg acuacgggaa ggccguggag      420

gagaaccgca agcuccuggc cgagcagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc agguccgcgg ggacuucacc gugguggaga acggcgagaa gugccggcuc      540

aucaacgucu ucuccaccag cgcguaccgc aaggaggccg agaagauccu gcggaagcag      600

caggaguuca acaaccagau cacggacgag uucaucgagg acuaccugaa gauccucacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccggaccga cuacgggcgc      720

uuccgcaccg acggcacgac ccuggacaac aucuucgggg ugcugaucgg caagugcacc      780

uucuacccca acgaguaccg ggccagcaag uccagcuaca ccgcccagga guucaaccuc      840

cugaacgacc ugaacaaccu cacggugccg accgagacca agaagcuguc cgaggagcag      900

aagaagguca ucguggagua cgccaaggag gcgaagaccc ugggggccag cacgcuccug      960

aaguacaucg ugaagaugau cgacgccucc gucgaccaga ucagcggcua ccgcguggac     1020

gugaacaaca agcccgagau gcacaccuuc gaggucuacc ggaagaugca gucccuggag     1080

accaucagcg ugggcgagcu cagccgcaac gugcuggacg agcuggccca cauccucacc     1140

cugaacacgg agcgggaggg gaucgaggag gccaucaacu ccaagcugau gggcgucuuc     1200

agccaggacc aggugcucga gcugguccag uuccgcaaga acaacuccag ccuguucucc     1260

aagggguggc acaacuucag ccucaagcug augauggagc ugauccccga gcucuacgag     1320

accuccgagg agcagaugac cauccugacc cggcugggca agcagaaguc caaggagacg     1380

agcaagcgca ccaaguacau cgacgagaag gagcucaccg aggagaucua caaccccgug     1440

guggccaaga gcguccggca ggcgaucaag aucaucaacg aggccaccaa gaagcacggg     1500

aucuucgaca acaucgugau cgagauggcc cgcgagaaca acgaggagga cgccaagaag     1560

gacuacauca agcggcagaa ggcgaaccag gacgagaaga acgccgccau ggagaaggcc     1620

gccuuccagu acaacggcaa gaaggagcug ccgggcaaca ucuucaacgg gcacaaggag     1680

cuggcgacga agauccgccu guggcaccag cagggcgaga agugccucua caccgggaag     1740

accaucccca ucuccgaccu gauccacaac caguacaagu acgagaucga ccacauccug     1800

ccccucagcc uguccuucga cgacagccug uccaacaagg ugcucgucca cgccaccgcc     1860

aaccaggaga agggccagcg gacgcccuuc caggcccugg acagcaugga cgacgcgugg     1920

uccuaccgcg aguucaagag cuacgugaag gaccugaagu cccucagcaa caagaagaag     1980

gacuaccugc ugaccgagga ggacaucagc aagaucgagg ugaagcagaa guucaucgag     2040

cggaaccucg ucgacacccg cuacuccagc cggguggugc ugaacgcccu gcaggacuuc     2100

uacaaguccc accaguucga caccacgauc agcgucgugc gcgggcaguu caccucccag     2160

cuccggcgca aguggggccu ggagaagucc cgcgagaccu accaccacca cgccguggac     2220

gcccugauca ucgccgcgag cagccagcuc cggcugugga agaagcagaa caacccgcug     2280

aucaccuaca aggagggcca guucgucgac ucccagaccg gggagaucau cagccucacg     2340

gacgacgagu acaaggagcu gguguucaag gcccccuacg accacuucgu ggacacccug     2400

cacuccaaga ccuucgagga cggcauccuc uucagcuacc aggucgacuc caaguacaac     2460

cgcaagauca gcgacgccac caucuacgcc acgcggaagg ccaagcugga caaggacaag     2520

uccgaggaga ccuacgugcu ggggaagauc aaggacaucu acagccaggc gggcuacgac     2580

gccuucauca agaucuacaa caaggacaag cgcaaguucc ucauguacca caaggacccc     2640

cagaccuucg agaaggugau cgaggagauc cugcggaccu accccuccaa ggagcgcaac     2700

gacaagaaca aggagguccc gugcaacccc uucgagaagu accggcagga gaacggcccc     2760

auccgcaagu acagcaagaa ggggaacggc cccgagauca agugccugaa guacuacgac     2820

aacaagcucg ggaacuacau cgacaucagc ccggacggcu ccgacaacca gguggugcug     2880

cagagccuga agcccuggcg gacggacguc uacuucaacc accagaccaa ggaguacgag     2940

cucauggggc ugaaguacuc cgaccugagc uucgagaagg gcuccggcaa guacagcauc     3000

uccaacgaga aguacaacag caucaagcgc aucgaggggg ugaacgagca guccgaguuc     3060

aaguucaccc ucuacaagaa cgaccugauc cugaucaagg acagcgagaa caacgagcag     3120

aagcuguucc gguucaacuc ccgcaacgac accgccaagc acuacgugga gcucaagccc     3180

uacgacaagg cccgguucga cggccagcag gagcugauca cgauccucgg gaacgucgcc     3240

aagggcgggc agugccugaa gggccugaac aagagcaaca ucuccaucuu caaggugaag     3300

accgacgugc ucggcaagaa gcacaucauc aagaaggagg gggacgagcc caagcugaag     3360

uucuga                                                                3366


<210>  1631
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1631
auggccgaca agacccuggg cgcccucacc uucggguucg acaucggcau cgcgagcgug       60

ggguggugcg uccugggcga gaaccgcauc guggaccugg gggugcgggc cuucgacaag      120

gccgagacgg ccaaggaggg cgagucccuc aaccuggccc gccggaccgc gcgccugaug      180

cggcgccggc uccgccggcg cgccuggcgg cugaccaagc uggcccgccu ccugaagcgg      240

gagggccuga ucgccgacgc guuccagaug caccagccca gcgccaccuc cccguggaag      300

cuccgcgucg aggcccugga ccggcgccug acggacgacg agugggcccg ggugaucuac      360

caccucugca agcaccgcgg guuccacugg aucagccggg ccgaggagaa gaaggcggag      420

ggcgacgcca agggggaguc cggccgcgug aagaaggggc ugagcgacac ccagcggcgc      480

augggcgaga agggcuaccg cuccgccgcc gagauggucc uggccgaguu ccccgaggcg      540

cagcggaaca agcaggggga cuacggcaag gcccucagcc gcgugcugcu guccgaggag      600

cucgcccugc uguuccggcg ccagcgggag cucgggcaca cccacgccag caaggagcug      660

gaggccgcga uccugggcaa cggcgaccgc aagggcggcc ucuucugggc ccagaagccg      720

ccccuggccg gggccgaccu gcucaagaug cuggggaagu gcaccuucga gaaggacgag      780

uaccgggccc ccaaggcguc cuucacggcc gagcgccacg uguggcugac ccggcucaac      840

aaccugcgcg ucguggugga cgggaccacc cggccccuga cggaggacga gcgccggguc      900

gcccuccccc ugcccuacgg cuacgccgac gcgaagcuga ccuacaagca gcuccgcgcc      960

gcccugagca aggccggccu guccaacuuc agcuucgccg ggaucgcgga cgacaaggag     1020

aaggacaccc ucaccaagcu gccggccugg gagacgcugu ccaagacccu caagggcaag     1080

gggcuggaga ccgaguggga gggcauggcc ggggccgcca ccggcggcga ccccacgcug     1140

cucgacgaga ucagccgggu gcuguccgug uacaaggacg gggacgaggu cgacguggag     1200

cugcgcaagc ugccccuccc cggcggggag aagauggugg agacccugag cgagaucucc     1260

uucgacaagu uccacgcgcu gagccucaag gcccugcgga agaucguccc gcacauggag     1320

accggccugc gcuacgacga ggccugcgcc gccgcggggu accaccacag ccagcuccac     1380

gaggccggcg ccggcgggca gaaguaccug ccgcccuucu acacccggcg cgacaaggac     1440

ggccgcaugg uguucgacga ggagcuggac gugccccgga accccgucgu gcuccgcgcc     1500

cugaaccagg cgcggaaggu gcugaacgcc cucguccgcg ccuacggguc ccccagcgcc     1560

gugcacaucg agauggcccg ggaccugucc aagccccugg ucggccaccc cacgccgaac     1620

ggcggguaca ucgagggccg ccgggacauc gagaagcucc agaaggagua ccgcgagcgg     1680

aacgagaagg acaaggcggc cuucgccgcc gagcacggga ucgccgccaa gagccgcgag     1740

uucgagaagu uccagcugua ccgggagcag cagggcaagu gcgccuacuc ccugaagccc     1800

cucgaccugc accgcgugcu gcacgacauc ggguacgccg agguggacca cgcccucccc     1860

uacagccggu ccuacgacga cggcaagaac aacaaggucc uggugcugac cgaggagaac     1920

cgcaacaagg gcaaccggac cgccuacgag uaccucugcg cguuccccgg gggcgagaac     1980

ggggagcgcu ggcggaacuu cguggccuac gucgagagca acaaguccua ccgccuggcc     2040

aagcggaccc gccugcuccg gaaggacuuc ggcgggaagg ccgcggagga guuccgcgag     2100

cgcaaccuga acgacacgcg guacaucugc aaguucuuca agaacuucgu ggagcgcuuc     2160

cugcagcuca aggugggcgc cgacggcacc gccgccaagc ggugcgucgu gcugagcggg     2220

cagaccaccg ccuuccugcg cgcgcggugg ggccuccuga aggugcgcgg ggccuccgac     2280

cggcaccacg cccuggacgc cgccgucgug gcggccugca gccacggcau ggugaagcgc     2340

cucgccgacu acucccggac gaaggagcug gccaaggucc gcgacggguu cccggacccc     2400

gagaccggcg agaucgugga ccccgccaug uuccagcagc ugcacgcgca cuuccccgac     2460

ccguggcccc acuuccgggg cgagcucgag gcccgccugc agcuggacga cagcgccgag     2520

cuccgggccc agcuggagau guucgggacc uaccccgccg aggcgcugga gacccuccgc     2580

ccgcuguucg ugucccgggc gccccagcgc cggaacggcg gggccgccca caaggagacg     2640

aucuacgcgc agcccgagcg ccugaaggag cagggcgggg ucacccagaa ggugagccuc     2700

gccucccuga agcccaccga caucgacaag cugaucgacc cgcaccggaa cgagaagcuc     2760

uacgccgcca uccgcgcccg ccuggaggcg cacggcggca agagcgacaa ggccuucccc     2820

gccgacaacc cccugcggaa gcccgaccgc gacgggaacc cgaccggccc caucgugcgg     2880

gccgucacca uggugaucga caagcucucc gggauccaga uccgcggcgg caucgccaag     2940

aacgacacga ugcugcgggu ggacgucuuc cgccacaaga aggacgggaa guuccaccug     3000

gugccccucu acgugcacca cgcggucgcc caggagcugc ccaaccgggc caucguggcc     3060

uucaaggacg aggacgagug gacccugauc gacgacaacu uccacuuccu cuucagcuuc     3120

cacccgaacg acuucauccg caucacccag aagaacaagg cccccauccu gggcuacuac     3180

gcgagcugcc accgggggac cggcaaccug aaccucuggg cccacgaccg caaccagaag     3240

guggggaagg acggccagau cgagggcauc ggggucaaga cggccguggc ccuggagaag     3300

uuccacgugg acguccuggg caacaucuac cccgcgcccc cggagcagcg gcacgggcuc     3360

gccuga                                                                3366


<210>  1632
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1632
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucguccggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgccggg ugcgccugaa ccgccucuuc gaggaguccg gccugaucac cgacuucacc      240

aagaucagca ucaaccugaa cccguaccag cuccggguga aggggcugac cgacgagcug      300

uccaacgagg agcuguucau cgcccugaag aacaugguca agcaccgggg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgcuaccag      480

accuacggcc agcugcgggg cgacuucacc gucgagaagg acgggaagaa gcaccgccuc      540

aucaacgugu uccccacguc cgccuaccgg agcgaggcgc ugcgcauccu gcagacccag      600

caggaguuca acccccagau caccgacgag uucaucaacc gguaccucga gauccugacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggacgga cuacggccgc      720

uaccgcacca gcggggagac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuaccccg acgaguuccg ggccgccaag gccuccuaca cggcgcagga guucaaccug      840

cugaacgacc ucaacaaccu gaccgugccc accgagacca agaagcugag caaggagcag      900

aagaaccaga ucaucaacua cgucaagaac gagaaggcca ugggccccgc caagcuguuc      960

aaguacaucg ccaagcuccu guccugcgac guggccgaca ucaaggggua ccgcaucgac     1020

aagagcggca aggcggagau ccacacguuc gaggccuacc ggaagaugaa gacccucgag     1080

acccuggaca ucgagcagau ggaccgcgag acccuggaca agcucgccua cgugcugacg     1140

cugaacaccg agcgggaggg cauccaggag gcccucgagc acgaguucgc cgacgggucc     1200

uucagccaga agcaggucga cgagcuggug caguuccgca aggcgaacag cuccaucuuc     1260

ggcaaggggu ggcacaacuu cagcgugaag cugaugaugg agcucauccc ggagcuguac     1320

gagaccuccg aggagcagau gaccauccug acgcggcucg gcaagcagaa gaccaccagc     1380

uccagcaaca agaccaagua caucgacgag aagcugcuga cggaggagau cuacaacccc     1440

gucguggcca aguccgugcg ccaggccauc aagaucguca acgccgccau caaggaguac     1500

ggggacuucg acaacaucgu gaucgagaug gcgcgggaga ccaacgagga cgacgagaag     1560

aaggccaucc agaagaucca gaaggccaac aaggacgaga aggacgccgc caugcucaag     1620

gcggccaacc aguacaacgg caaggccgag cugccccaca gcguguucca cggccacaag     1680

cagcuggcca ccaagauccg ccuguggcac cagcaggggg agcggugccu cuacaccggc     1740

aagacgaucu ccauccacga ccugaucaac aacagcaacc aguucgaggu cgaccacauc     1800

cugccccucu ccaucaccuu cgacgacagc cuggcgaaca aggugcuggu cuacgccacc     1860

gccaaccagg agaaggggca gcgcaccccg uaccaggccc ucgacuccau ggacgacgcc     1920

uggagcuucc gggagcugaa ggcguucgug cgcgagucca agacccugag caacaagaag     1980

aaggaguacc uccugacgga ggaggacauc uccaaguucg acgugcggaa gaaguucauc     2040

gagcgcaacc uggucgacac ccgguacgcc agccgcgugg ugcucaacgc ccugcaggag     2100

cacuuccggg cccacaagau cgacaccaag gucuccgugg ugcgcggcca guucaccagc     2160

cagcugcgcc ggcacugggg gaucgagaag acgcgcgaca ccuaccacca ccacgccguc     2220

gacgcgcuca ucaucgccgc cagcucccag cugaaccugu ggaagaagca gaagaacacc     2280

cucgugagcu acuccgagga ccagcugcug gacaucgaga ccggcgagcu caucagcgac     2340

gacgaguaca aggaguccgu guucaaggcc cccuaccagc acuucgucga cacgcucaag     2400

agcaaggagu ucgaggacuc cauccuguuc agcuaccagg uggacuccaa guucaaccgg     2460

aagaucagcg acgccaccau cuacgcgacc cgccaggcca aggugggcaa ggacaaggcc     2520

gacgagaccu acguccucgg gaagaucaag gacaucuaca cgcaggacgg cuacgacgcc     2580

uucaugaaga ucuacaagaa ggacaagucc aaguuccuga uguaccggca cgacccccag     2640

accuucgaga aggugaucga gcccauccug gagaacuacc cgaacaagca gaucaacgag     2700

aaggggaagg aggugcccug caaccccuuc cucaaguaca aggaggagca cggcuacauc     2760

cgcaaguaca gcaagaaggg caacgggccc gagaucaagu cccugaagua cuacgacagc     2820

aagcugggca accacaucga caucaccccg aaggacucca acaacaaggu cgugcuccag     2880

agcguguccc ccuggcgggc ggacgucuac uucaacaaga ccacggggaa guacgagauc     2940

cugggccuga aguacgccga ccuccaguuc gagaagggga ccggcaccua caagaucagc     3000

caggagaagu acaacgacau caagaagaag gagggcgugg acagcgacuc cgaguucaag     3060

uucacccugu acaagaacga ccugcuccug gugaaggaca cggagaccaa ggagcagcag     3120

cuguuccgcu uccucagccg gaccaugccc aagcagaagc acuacgucga gcugaagccc     3180

uacgacaagc agaaguucga gggcggggag gcccugauca aggugcucgg gaacguggcc     3240

aacuccggcc agugcaagaa ggggcugggc aagagcaaca ucuccaucua caagguccgc     3300

accgacgugc ugggcaacca gcacaucauc aagaacgagg gggacaagcc gaagcucgac     3360

uucuga                                                                3366


<210>  1633
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1633
augaacggcc uggugcucgg gcuggacauc ggcaucggga gcgucggcgu ggggauccug       60

aacaaggaca ccggcgagau cauccacgcc aacucccgca ucuuccccgc cgcgaccgcc      120

gacaacaacg ugguccggcg cagcucccgg cagggccgcc ggcucaacca ccgcaagaag      180

caccggagcg ugcgccugaa ggaccuguuc gaguacuacg accuccugac ggacuucucc      240

aaggugagca ucaaccugaa cccguaccgg cuccgcgucg acgggcugaa ccagcagcug      300

accaacgagg agcuguucau cgcccucaag aacaucguga agcggcgcgg caucuccuac      360

cuggacgacg ccagcgagga cgggggcgcc guguccagcg acuacgggaa ggcggucgag      420

gagaaccgga agcuccuggc cgagcagacc cccggccaga uccagcugga gcgcuucgag      480

aaguacggcc aggugcgcgg ggacuucacc guggucgaga acggcgagaa gcggcggcuc      540

aucaacgugu ucuccacgag cgccuacaag aaggaggccg agcgcauccu gcggaagcag      600

caggaguaca acaaccagau cauggacgag uucaucgcgg acuaccugac cauccucacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccggaccga cuacgggcgc      720

uuccgcacgg acggcaccac ccuggacaac aucuucggga uccugaucgg caagugcacc      780

uucuaccccg aggaguaccg ggccagcaag gccuccuaca cggcccagga guucaaccuc      840

cugaacgacc ugaacaaccu caccgugccg accgagacca agaagcugag cgaggagcag      900

aagaagacga ucgucgagua cgccaagucc gcgaagaccc ugggggccag cacccuccug      960

aaguacaucg ccaagaugau cgacgccucc guggaccaga uccgcggcua ccggguggac     1020

gucaacaaca agcccgagau gcacaccuuc gagguguacc gcaagaugca gcugcucgag     1080

acgauccccg ugggcgaguu cagccgggag guccuggacg agcuggccca cauccucacc     1140

cugaacaccg agcgcgaggg gaucgaggag gcgaucaaca ccaagcugau ggacguguuc     1200

agccaggacc agauccucga gcuggugcag uuccggaaga acaacuccag ccuguucucc     1260

aagggcuggc acaacuucag ccucaagcug augaucgagc ugauccccga gcucuacgag     1320

acguccgagg agcagauggc cauccugacc cgccugggga agcagaaguu caaggagacc     1380

agcaagcgga ccaaguacau cgacgagcag gaggucacgg aggagaucua caacccggug     1440

gucgccaagu ccgugcgcca ggccaucaag aucaucaacg aggccaccaa gaaguacggc     1500

aucuucgaca acaucgugau cgagauggcg cgggagaaca acgaggagga ggccaagaag     1560

gacuacauca agcgccagaa ggccaaccac gacgagaaga acgccgccau ggagaaggcg     1620

gccuuccagu acaacgggga gaaggagcuc cccgacagca ucuuccacgg ccacaaggag     1680

cuggccacca agguccggcu guggcaccag cagggcgaga agugccugua caccgggaag     1740

aacaucccca ucuccgaccu cauccacaac caguacaagu acgagaucga ccacauccug     1800

ccccugagcc ucuccuucga cgacagccug uccaacaagg ugcuggugcu cgccacggcg     1860

aaccaggaga agggccgccg gaccccguuc caggcccugg acagcaugga cgacgccugg     1920

uccuaccgcg aguucaagag cuacgucaag gacuccaaga gccuggggaa caagaagaag     1980

gacuaccucc ugaccgagga ggacaucucc aagaucgagg ugaagcagaa guucaucgag     2040

cggaaccugg uggacacccg cuacagcagc cgggucgugc ucaacacgcu gcaggacuuc     2100

uacaaggccc acaaguucga caccaccauc uccguggucc gcggccaguu caccagccag     2160

cugcgccgga agugggggcu cgagaagucc cgcgagaccu accaccacca cgccguggac     2220

gcgcugauca ucgccgccag cucccagcug cggcucugga agaagcagaa caacccccug     2280

aucagcuaca aggagggcca guucguggac uccgagacgg gcgagaucau cagccugacc     2340

gacgacgagu acaaggagcu cgucuucaag gcccccuacg accacuucgu ggacacccug     2400

cgcuccaaga aguucgagga cagcauccug uucuccuacc agguggacag caaguucaac     2460

cggaagaucu ccgacgccac caucuacgcg acgcgcaagg ccaagcucga caaggacaag     2520

agcgaggaga ccuacguccu ggggaagauc aaggacaucu acucccagag cggcuacgac     2580

gccuucauca agaucuacaa caaggacaag uccaaguucc ugauguacca caaggacccc     2640

cagaccuucg agaaggugau cgaggagauc cuccggaccu acccgagcaa ggaguucaac     2700

gagaagaaca aggaggugag cugcaacccc uucgagaagu acaagcagga gaacgggccc     2760

auccgcaagu acuccaagaa gggcaacggc cccgagauca agagccugaa guacuacgac     2820

aagaacaucc cggaguccuu caucaacauc acgcccagca acuccaagaa caaggucauc     2880

cugcggcagc ucaaccccug gcgcaccgac guguacuuca accaccagac cgagaaguac     2940

gagcugcugg ggcucaagua cgccgaccug caguucgaga agaagaccgg cacguacagc     3000

aucaccaucg acaaguacaa cgagaucaag gagaaggagg ggguguccga gcacagcgag     3060

uucaaguuca cccuguacaa gaacgaccuc cugcugauca aggacaccga gaucaaggag     3120

gagcagaucu uccgguuccu cucccgcacg augcccaacc agaagcacua cgucgagcug     3180

aagccguacu acaagggcaa cuucgagggg aaggagcgga ucaagguguu caacaccacc     3240

agcaacggcc gcugccugaa gggccuccgg aagaaggggc uguccaucua caagaucaag     3300

accgacgugc ugggcaacaa gcacuucauc aagaaggagg gggagaagcc caagcucaag     3360

uucuga                                                                3366


<210>  1634
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1634
augaacggca agauccuggg gcucgacauc ggcguggcca gcgucggggu gggcauccug       60

gacaagaaga ccggggagau cauccacgcc uccagccgca ucuuccccgc ggccaccgcc      120

gacuccaacg uggagcggcg cggcuuccgg cagggccgcc ggcuggggcg ccggaagaag      180

caccgcaagg uccggcucgc cgaccuguuc agcgacacgg gccugaucac cgacuucucc      240

aaggugagca ucaaccucaa cccguacgag cugcgcauca aggggcugaa cgagaagcuc      300

accaacgagg agcuguucau cgcccugaag aacaucguga agcggcgcgg caucuccuac      360

cucgacgacg cgaacgagga cggggagagc uccagcuccg aguacggcaa ggccgucgag      420

gagaaccgga agcugcuggc cgacaagacc cccggccaga uccagcucga gcgcuucgag      480

aaguacgggc agguccgggg cgacuucacg aucgaggaga acggggagaa gcaccgccug      540

cugaacgugu ucagcaccuc cgccuacaag aaggaggcgg agcggauccu caccaagcag      600

caggacuaca accaggacau caccgacgag uucauccagg ccuaccugac gauccugacc      660

ggcaagcgca aguacuacca cggccccggg aacgagaagu cccggaccga cuacggccgc      720

uuccggaccg acgggacgac ccucgacaac aucuucggca uccugaucgg gaagugcacc      780

uucuaccccg aggaguaccg cgccgccaag gccagcuaca ccgcgcagga guucaaccug      840

cucaacgacc ugaacaaccu gacggugccg accgagacca agaagcucag cgaggagcag      900

aagcgccaga ucaucgagua cgccaagggc gccaagaccc ugggcgccgc cacgcugcuc      960

aaguacaucg cgaagcuggu ggacgggucc guggaggaca ucaagggcua ccggaucgac     1020

aagagcgaga agcccgagau gcacaccuuc gacaucuacc gcaagaugca gacccuggag     1080

accgucgacg uggagaagcu cucccgggag gugcuggacg agcuggccca cauccucacg     1140

cugaacaccg agcgcgaggg gaucgaggag gccaucaagg ucagcuucau caagcgggag     1200

uucgagcagg accagaucgc cgagcuggug agcuuccgca aguccaacag cucccucuuc     1260

ggcaaggggu ggcacaacuu cagcaucaag cugaugaccg agcugauccc cgagcucuac     1320

gagaccuccg aggagcagau gacgauccug acccggcugg gcaagcagaa gaccaaggcc     1380

cgcagcaagc ggaccaagua caucgacgag aaggagcuca cggacgagau cuacaacccc     1440

guggucgcga aguccgugcg ccaggccauc aagaucauca accuggccac caagaaguac     1500

ggcguguucg acaacaucgu caucgagaug gcccgggaga acaacgagga ggacgccaag     1560

aaggacuacg ugaagcgcca gaaggcgaac gaggacgaga agaacgccgc cauggagaag     1620

gccgcgcacc aguacaacgg gaagaaggag cugccggaca acguguucca cggccacaag     1680

gagcucgcca ccaagauccg gcuguggcac cagcaggggg agaagugccu guacaccggc     1740

aagaacaucc ccaucagcga ccugauccac aaccaguaca aguacgagau cgaccacauc     1800

cucccccugu cccugagcuu cgacgacucc cucgccaaca agguccuggu gcuggccacg     1860

gccaaccagg agaaggggca gcgcaccccc uuccaggcgc ucgacagcau ggacgacgcc     1920

ugguccuacc gggaguucaa ggccuacguc cgcggcgccc gggcccugag caacaagaag     1980

aaggacuacc ugcucaacga ggaggacauc aacaagaucg aggugaagca gaaguucauc     2040

gagcgcaacc ugguggacac ccgguacucc agccgcgucg ugcugaacgc gcuccaggac     2100

uucuacaagc ugaacgacuu cgacaccaag aucuccgugg uccgcggcca guucaccagc     2160

cagcugcggc gcaaguggcg gaucgacaag ucccgcgaga cguaccacca ccacgccgug     2220

gacgcccuca ucaucgccgc cagcagccag cugcggcugu ggaagaagca ggggaacccg     2280

cucaucuccu acaaggagaa ccaguucgug gacagcgaga ccggcgagau caucucccug     2340

accgacgacg aguacaagga gcuggucuuc cgcgcgcccu acgaccacuu cguggacacc     2400

gugagcucca agaaguucga ggaccggauc cucuucagcu accaggucga cuccaaguac     2460

aaccgcaaga ucagcgacgc cacgaucuac uccacccgga aggccaagcu ggggaaggac     2520

aagagcgagg agaccuacgu gcugggcaag aucaaggaca ucuacaccca gacgggcuac     2580

gacgccuuca ucaagcucua caagaaggac aaguccaagu uccugaugua ccacaaggac     2640

cccaucaccu ucgagaaggu gaucgaggag auccugaaga ccuaccccga caaggagauc     2700

aacgagaagg ggaaggaggu cgcgugcaac ccguucgaga aguaccgcca ggagaacggc     2760

ccccuccgga aguacagcaa gaaggggaag ggccccgaga ucaagucccu gaaguacuac     2820

gacaacaagc uggggaacca caucgacauc acccccgaca acagcgagaa ccaggugauc     2880

cuccaguccc ugaagccgug gcgcacggac guguacuuca accacaagac caagaucuac     2940

gagcugaugg gccucaagua cagcgaccug uccuucgaga agggcagcgg gaaguaccgg     3000

aucagccugg acaaguacaa cgucaucaag aagaaggagg gcgugcacaa ggaguccgag     3060

uucaaguuca cccucuacaa gaacgaccug auccugauca aggaccucga gaaguccgag     3120

cagcagcugu uccgcuacaa cagccggaac gacaccagca agcacuacgu ggagcugaag     3180

cccuacgaca aggccaaguu cgaggggaac cagccccuca uggcccuguu cggcaacguc     3240

gccaagggcg ggcagugccu gaagggccuc aacaaggcca acaucuccau cuacaaggug     3300

cagacggacg ugcuggggaa caagcgcuuc aucaagaagg agggcgacgc gcccaagcug     3360

gaguucuga                                                             3369


<210>  1635
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1635
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucguccggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgcaucg ugcggcugaa ccgccucuuc gaggaguccg gccugaucac cgacuucacc      240

aagaucagca ucaaccugaa cccguaccag cuccggguga aggggcugac cgacgagcug      300

uccaacgagg agcuguucau cgcccucaag aacaugguca agcaccgcgg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgguaccag      480

accuacggcc agcugcgcgg cgacuucacc gucgagaagg acgggaagaa gcaccggcuc      540

aucaacgugu uccccacguc cgccuaccgc agcgaggcgc ugcggauccu gcagacccag      600

caggaguuca acucccagau caccgacgag uucaucaacc gcuaccucga gauccugacc      660

ggcaagcgga aguacuacca cgggcccggc aacgagaagu cccgcacgga cuacggccgg      720

uaccgcacca acggggagac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuacccgg acgaguuccg cgccgccaag gccagcuaca cggcgcagga guucaaccug      840

cugaacgacc ucaacaaccu gaccgugccc accgagacca agaagcugag caaggagcag      900

aagaaccaga ucaucaacua cgucaagaac gagaagguga ugggccccgc caagcuguuc      960

aaguacaucg ccaagcuccu guccugcgac guggccgaca ucaaggggca ccggaucgac     1020

aagagcggca aggccgagau ccacacguuc gaggcguacc gcaagaugaa gacccucgag     1080

acccuggaca ucgagcagau ggaccgggag acccuggaca agcucgccua cguccugacg     1140

cugaacaccg agcgcgaggg cauccaggag gcccucgagc acgaguucgc cgacgggucc     1200

uucagccaga agcaggucga cgagcuggug caguuccgga aggccaacag cuccaucuuc     1260

ggcaaggggu ggcacaacuu cagcgugaag cugaugaugg agcucauccc cgagcuguac     1320

gagaccuccg aggagcagau gaccauccug acgcgccucg gcaagcagaa gaccaccagc     1380

uccagcaaca agaccaagua caucgacgag aagcugcuga cggaggagau cuacaacccg     1440

gugguggcga aguccguccg gcaggccauc aagaucguga acgccgccau caaggaguac     1500

ggggacuucg acaacaucgu gaucgagaug gcccgcgaga ccaacgagga cgacgagaag     1560

aaggcgaucc agaagaucca gaaggccaac aaggacgaga aggacgccgc caugcucaag     1620

gccgcgaacc aguacaacgg caaggccgag cugccccaca gcgucuucca cggccacaag     1680

cagcuggcca ccaagauccg gcuguggcac cagcaggggg agcgcugccu cuacaccggc     1740

aagacgaucu ccauccacga ccugaucaac aaccccaacc aguucgaggu ggaccacauc     1800

cugccccuca gcaucaccuu cgacgacucc cuggccaaca agguccuggu guacgcgacc     1860

gccaaccagg agaaggggca gcggaccccg uaccaggccc ucgacagcau ggacgacgcc     1920

ugguccuucc gcgagcugaa ggccuucgug cgggagagca agacccuguc caacaagaag     1980

aaggaguacc uccugacgga ggaggacauc agcaaguucg acguccgcaa gaaguucauc     2040

gagcggaacc ugguggacac ccgcuacgcg ucccgggugg uccucaacgc ccugcaggag     2100

cacuuccgcg cccacaagau cgacaccaag gugagcgugg uccggggcca guucaccucc     2160

cagcugcgcc gccacugggg gaucgagaag acgcgggaca ccuaccacca ccacgccgug     2220

gacgcccuca ucaucgcggc cagcagccag cugaaccugu ggaagaagca gaagaacacc     2280

cucguguccu acagcgagga gcagcugcug gacaucgaga ccggcgagcu caucuccgac     2340

gacgaguaca aggagagcgu cuucaaggcc cccuaccagc acuucgugga cacgcugaag     2400

uccaaggagu ucgaggacag cauccuguuc uccuaccagg uggacagcaa guucaaccgc     2460

aagaucuccg acgccaccau cuacgccacc cggcaggcga aggucggcaa ggacaagaag     2520

gacgagaccu acgugcucgg gaagaucaag gacaucuaca cgcaggacgg cuacgacgcc     2580

uucaugaaga ucuacaagaa ggacaagagc aaguuccuga uguaccgcca cgacccccag     2640

accuucgaga aggugaucga gcccauccug gagaacuacc cgaacaagga gaugaacgag     2700

aaggggaagg agguccccug caaccccuuc cucaaguaca aggaggagca cggcuacauc     2760

cggaaguacu ccaagaaggg caacgggccc gagaucaaga gccugaagua cuacgacucc     2820

aagcugcucg gcaacccgau cgacaucacc cccgagaaca gcaagaacaa gguggugcug     2880

cagucccuga agcccuggcg caccgacguc uacuucaaca agaacacggg gaaguacgag     2940

auccucggcc ugaaguacgc cgaccugcag uucgagaaga agaccgggac cuacaagauc     3000

agccaggaga aguacaacgg caucaugaag gaggagggcg uggacuccga cagcgaguuc     3060

aaguucaccc ucuacaagaa cgaccugcug cucgugaagg acacggagac caaggagcag     3120

cagcuguucc gguuccugag ccgcaccaug cccaacguca aguacuacgu ggagcucaag     3180

ccguacucca aggacaaguu cgagaagaac gagagccuga ucgagauccu gggguccgcc     3240

gacaagagcg gccggugcau caaggggcuc ggcaagucca acaucagcau cuacaaggug     3300

cgcaccgacg uccuggggaa ccagcacauc aucaagaacg agggcgacaa gcccaagcug     3360

gacuucuga                                                             3369


<210>  1636
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1636
augagcaacg gcaagauccu ggggcucgac aucggcaucg ccuccguggg ggucggcgug       60

aucgacgccc agaccgggga gaucauccac gcgagcuccc gcaucuuccc cagcgccaac      120

gccgccaaca acgccgagcg gcgcaccuuc cggggcuccc gccggcugau ccgccggaag      180

aagcaccgca ucaagcggcu ggacgaccuc uucaacgacu uccacaucaa ccuggacggc      240

gagaugagca cggacaaccc guacgugcug cgcgucaagg ggcucuccca gaagcugacc      300

guggaggagc uguacaucag caucaagaac aucaugaagc ggcgcggcau cuccuaccuc      360

gacgacgcgg agagcgacaa cgaggccggg cgguccgacu acgccaaggc caucgagcgc      420

aaccggcagc ugcugaccag caagaccccc ggcgagaucc agcucgagcg ccuggagaag      480

uacgggcagc ugcggggcaa cuucacgauc aucgacgagg agggccaguc ccagcagauc      540

aucaacgugu ucagcaccuc cgacuacguc aaggaggugg agaagauccu cgacugccag      600

aagauguacc acaaguucau cagcgacgag uucugcgaca agcugaucga gcugcuccgc      660

gagaagcgga aguacuacgu ggggcccggc aacgagaagu cccgcaccga cuacgggauc      720

uaccggaccg acggcacgac ccuggagaac cuguucggca uccucaucgg gaagugcacc      780

uucuaccccg accaguaccg cagcucccgc gcgagcuaca ccgcccagga guucaacuuc      840

cugaacgacc ugaacaaccu cacggucccg accgagacca agaagcugag ccaggagcag      900

aaggaguucc uggugaacua cgccaaggag accuccgugc ucggcgccgg gaagauccug      960

cagcagaucg ccaagcuggc ggacugcaag gucgaggaca uccggggcua ccgccucgac     1020

aacaaggaca agcccgagcu gcacaucuuc gagacguacc gggccaugaa ggggcuggug     1080

ccccucgugg acaucggcgu ccugagccgc gagcagcugg acauccucgc cgacauccug     1140

acccugaaca ccgacuucga gggcauccgg gaggcccuca agaagcagcu gcccaacgug     1200

uucgacgaga agcaggucaa ggggcuggcc uccuuccgca agagcaaguc ccagcuguuc     1260

gcgaagggcu ggcacaaccu cagccagaag aucaugcugg aggugauccc ggagcucuac     1320

gccaccuccg acgagcagau gacgauccug acccggcugg ggaaguucga gaaguccagc     1380

guggccgagu accccagcuc caucaacgug gacgagauca ccgacgagau cuacaacccc     1440

gucguggcca agagcauccg ccagaccauc aagaucauca acgccuccau caagaagugg     1500

ggcgaguucg accagaucgu gaucgagaug ccccgggacc gcaacgagga cgaggagaag     1560

aagcggaucg cggacgggca gaaggccaac gccaaggaga aggccgacag cauccuccgc     1620

gccgcggagc uguacugcgc cggcaagguc cugccggacu acguguacaa cggccacaac     1680

cagcuggcca cgaagauccg gcucugguac cagcaggggg agcgcugcau cuacaccggc     1740

cagcccaucu ccauccacga ccugauccac aaccagaacc aguacgagau cgaccacauc     1800

cugccccuca gccugaccuu cgacgacucc cugagcaaca agguccucgu gcuggccacc     1860

gcgaaccagg agaaggccca gcggacgccc uacaacuacc ugaaguccgc caccagcgcc     1920

uggagcuacc gcgaguucaa ggacuacgug accaagcgga aggggaucgg caagaagaag     1980

ugcgaguacc ucaccuucga ggaggacauc aacggguucg agguccgcuc caaguucauc     2040

cagcggaacc ugguggacac gcgcuacgcc agcaagguga uccugaacgc gcuccaggac     2100

uacuucaaga ucuccggcau ccagaccaag gucagcgugg ugcgcggcca guucaccucc     2160

cagcugcggc acaagugggg gaucgagaag acccgcgaga cguaccacca ccacgccguc     2220

gacgcccuga ucaucgccgc cagcucccag cuccggcugu ggaagaagca ggagagcccg     2280

cugguggugg acuaccagga gggccgccag gucgaccucg agaccgggga gauccuggag     2340

cugaccgacg agcaguacaa ggagcucgug uaccagccgc ccuaccaggg cuucgugaac     2400

accaucucca gcuccgcguu cgacaacgag auccuguuca gcuaccaggu cgacuccaag     2460

gugaaccgga agaucagcga cgccaccauc uacgccacgc gcaacgccca gcugggcaag     2520

gacaagaccg aggggaucua cgugcucggc aagaucaagg acaucuacac ccaggccggg     2580

uacgaggcgu uccugaagcg guacaccaag gacaagacgu ccuuccugau guaccacaag     2640

gaccucgaca ccugggagaa ggucaucgag aucauccugc gcgacuaccg ggaguacgac     2700

gagaagggca aggagaucgg gaaccccuuc gagcgcuacc ggcgcgagaa cggcuacgug     2760

aagaaguaca gccggaaggg caacgggacc gccaucaagu cccugaagua cuacgacaac     2820

aagcucggca accacaucga caucaccccc gagaacagcc gcaacgccgu gguccugcag     2880

agccugaagc ccuggcggac ggacguguac uucaacaagg agaccgggaa guacgaguuc     2940

cucggcauca aguacuccga ccugagcuuc gagaagggga ccggcgagua cggcaucucc     3000

caggagaagu acgacagcau caagaucgcc gagggggugg cgaagaaguc caucuucaag     3060

uucacccugu acaagcagga ccuccuguuc aucaaggaca ucgagaacaa cuucggcaag     3120

cugcuccgcu ucacgagcaa gaacgacacc uccaagcacu acgucgagcu gaagcccuac     3180

gacaagaaca aguucgggac cgaggagccc cugcucccgg ugcugggcaa cguggccaag     3240

agcgggcagu gcaucaaggg ccugaacaag uccaacauca gcaucuacaa gguccggacc     3300

gacauccucg gcuaccgcca cuucaucaag caggaggggg agcaccccca gcugaaguuc     3360

aagaaguga                                                             3369


<210>  1637
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1637
augagcaacg gcaagauccu ggggcucgac aucggcaucg ccuccguggg ggucggcgug       60

aucgacgccc agaccgggga gaucauccac gcgagcuccc gcaucuuccc cagcgccaac      120

gccgccaaca acgccgagcg gcgcaccuuc cggggcuccc gccggcugau ccgccggaag      180

aagcaccgca ucaagcggcu ggacgaccuc uucaacgacu uccacaucaa ccuggacggc      240

gagaugagca cggacaaccc guacgugcug cgcgucaagg ggcucuccca gaagcugacc      300

guggaggagc uguacaucag caucaagaac aucaugaagc ggcgcggcau cuccuaccuc      360

gacgacgcgg agagcgacaa cgaggccggg cgguccgacu acgccaaggc caucgagcgc      420

aaccggcagc ugcugaccag caagaccccc ggcgagaucc agcucgagcg ccuggagaag      480

uacgggcagc ugcggggcaa cuucacgauc aucgacgagg agggccaguc ccagcagauc      540

aucaacgugu ucagcaccuc cgacuacguc aaggaggugg agaagauccu cgacugccag      600

aagauguacc acaaguucau cagcgacgag uucugcgaca agcugaucga gcugcuccgc      660

gagaagcgga aguacuacgu ggggcccggc aacgagaagu cccgcaccga cuacgggauc      720

uaccggaccg acggcacgac ccuggagaac cuguucggca uccucaucgg gaagugcacc      780

uucuaccccg accaguaccg cagcucccgc gcgagcuaca ccgcccagga guucaacuuc      840

cugaacgacc ugaacaaccu cacggucccg accgagacca agaagcugag ccaggagcag      900

aaggaguucc uggugaacua cgccaaggag accuccgugc ucggcgccgg gaagauccug      960

cagcagaucg ccaagcuggc ggacugcaag gucgaggaca uccggggcua ccgccucgac     1020

aacaaggaca agcccgagcu gcacacguuc gagaccuacc gggccaugaa ggggcuggug     1080

ccccucgugg acaucggcgu ccugagccgc gagcagcugg acauccucgc cgacauccug     1140

acccugaaca ccgacuucga gggcauccgg gaggcccuca agaagcagcu gcccaacgug     1200

uucgacgaga agcaggucaa ggggcuggcc uccuuccgca agagcaaguc ccagcuguuc     1260

gcgaagggcu ggcacaaccu cagccagaag aucaugcugg aggugauccc ggagcucuac     1320

gccacguccg acgagcagau gaccauccug acccggcugg ggaaguucga gaaguccagc     1380

guggccgagu accccagcuc caucaacgug gacgagauca ccgacgagau cuacaacccc     1440

gucguggcca agagcauccg ccagacgauc aagaucauca acgccuccau caagaagugg     1500

ggcgaguucg accagaucgu gaucgagaug ccccgggacc gcaacgagga cgaggagaag     1560

aagcggaucg cggacgggca gaaggccaac gccaaggaga aggccgacag cauccuccgc     1620

gccgcggagc uguacugcgc cggcaagguc cugccggacu acguguacaa cggccacaac     1680

cagcuggcca ccaagauccg gcucugguac cagcaggggg agcgcugcau cuacaccggc     1740

cagcccaucu ccauccacga ccugauccac aaccagaacc aguacgagau cgaccacauc     1800

cugccccuca gccugaccuu cgacgacucc cugagcaaca agguccucgu gcuggccacg     1860

gcgaaccagg agaaggccca gcggaccccc uacaacuacc ugaaguccgc caccagcgcc     1920

uggagcuacc gcgaguucaa ggacuacgug accaagcgga aggggaucgg caagaagaag     1980

ugcgaguacc ucacguucga ggaggacauc aacggguucg agguccgcuc caaguucauc     2040

cagcggaacc ugguggacac ccgcuacgcc agcaagguga uccugaacgc gcuccaggac     2100

uacuucaaga ucuccggcau ccagaccaag gucagcgugg ugcgcggcca guucaccucc     2160

cagcugcggc acaagugggg gaucgagaag acgcgcgaga ccuaccacca ccacgccguc     2220

gacgcccuga ucaucgccgc cagcucccag cuccggcugu ggaagaagca ggagagcccg     2280

cugguggugg acuaccagga gggccgccag gucgaccucg agaccgggga gauccuggag     2340

cugaccgacg agcaguacaa ggagcucgug uaccagccgc ccuaccaggg cuucgugaac     2400

accaucucca gcuccgcguu cgacaacgag auccuguuca gcuaccaggu cgacuccaag     2460

gugaaccgga agaucagcga cgccacgauc uacgccaccc gcaacgccca gcugggcaag     2520

gacaagaccg aggggaucua cgugcucggc aagaucaagg acaucuacac ccaggccggg     2580

uacgaggcgu uccugaagcg guacacgaag gacaagaccu ccuuccugau guaccacaag     2640

gaccucgaca ccugggagaa ggucaucgag aucauccugc gcgacuaccg ggaguacgac     2700

gagaagggca aggagaucgg gaaccccuuc gagcgcuacc ggcgcgagaa cggcuacgug     2760

aagaaguaca gccggaaggg caacgggacc gccaucaagu cccugaagua cuacgacaac     2820

aagcucggca accacaucga caucacgccc gagaacagcc gcaacgccgu gguccugcag     2880

agccugaagc ccuggcggac cgacguguac uucaacaagg agaccgggaa guacgaguuc     2940

cucggcauca aguacuccga ccugagcuuc gagaagggga ccggcgagua cggcaucucc     3000

caggagaagu acgacagcau caagaucgcc gagggggugg cgaagaaguc caucuucaag     3060

uucacgcugu acaagcagga ccuccuguuc aucaaggaca ucgagaacaa cuucggcaag     3120

cugcuccgcu ucaccagcaa gaacgacacc uccaagcacu acgucgagcu gaagcccuac     3180

gacaagaaca aguucgggac cgaggagccc cugcucccgg ugcugggcaa cguggccaag     3240

agcgggcagu gcaucaaggg ccugaacaag uccaacauca gcaucuacaa gguccggacg     3300

gacauccucg gcuaccgcca cuucaucaag caggaggggg agcaccccca gcugaaguuc     3360

aagaaguga                                                             3369


<210>  1638
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1638
auggccaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucgac       60

gacgugcaga acaagauccu cggggucggc ucccgcaucu uccccauggg gguggagaac      120

cugggcgacg gcgacgggga gaucagcaag aacgcguccc ggaccggcac gcgcggggug      180

cggcgccagu ucuuccggcg ccggcugcgc aagaaggucc uccugaaggc ccugagcgag      240

aacaacaugu gcccgaugau caccuccgac uucgacaacu ggaagaagac caagcaguuc      300

cccagcgaga agcucgccuc cugguucgcc cugaaccccu acgagcugcg gaacaaggcc      360

cucagcgaga agcugacccu ggaggagauc ggccgcaucu ucuaccaccu cauccagcgg      420

cgcggguucc uguccaacag ccggaagggc ggcacggacg acggggcgau cuucaagggc      480

aaccccaagg aggggaagau cggcaucacc gacacccagg aguccaucca ggacaagacc      540

cugggcagcu accucuucga gaucuacccg aaggagaacc agcccuucca gaacgggcug      600

gagcgcaucc ggaaccgcua cacggugcgg aagauguacg uggacgaguu cgagaugauc      660

ugggagaagc aggcccagua cuacuccaac cugaacgagg accucaagac ccuguucggc      720

gggcgcaagc uggacggcua caaggaggac gggauccucu uccaccagcg gccccugcgc      780

agccagaagc accuggucgg caacugcgug uucgagccca acaagaccaa gugcccgauc      840

uccgccaucc ccuucgagca guuccgggug uggcaguggg ucaacaccgu ggaguacaac      900

ggcaagaaga ucagccaggu ggagaaggag aagaucgucg aguuccucua cgccaacgac      960

aagacggacu ucaagaagau ccgcaaggcg aucgggaagg aguccaccga guucaaguuc     1020

aacuacaagg acgacgacaa gaucgugggc accuacaccg ugagccaccu guccaacaag     1080

aaguacuucg ggaagaagug guucgaguuc agcgagaagg agcaggagga caucuggcac     1140

auccuguacu ucuucgacag caaguccaac cucaaggagu acgccaucaa gaacuggaac     1200

uucacggagg agcaggccga gagcaucgcc aaguucaacg ucaaggacgg cuacuccagc     1260

cugucccgca aggccaucag caacauccug cccuuccuca agcugggguu caccuacgac     1320

guggcggugg uccugggcgg caucaagaac guguucgggc acgaguggga gaagcucucc     1380

gacgagaagc ggaacuuccu gaucgacaac guggagggca ucguccgcag caagaccaag     1440

ggcggguuca ucgacgugau caaggacauc cugcggaacg acuacaacau cuccgacaac     1500

cagcuccgca agcuguacca ccacagcgcc accaucuacg uggccgagcu gcucgagaag     1560

cugcccgucg ggaaggaggc cgacaaggag auccagucca uccggaaccc gaucgugauc     1620

acggcccugu ucgagcuccg caagcugguc aacgagcuga ucgacgacuu cggcagcauc     1680

gacgagauca agguggagau ggcgcgggac cucaagaucu ccaagagcca gcgcaacaag     1740

auccggcgcg agcagaagcg gcuggagcgc gagaacgacc gggugaaggc ccgccugcug     1800

gaggagggcc agcggaucac ccacgacaac auccuccugu acaagcugug ggaggagugc     1860

aagaagaccu gccccuacac cgggaacccc aucccccucu acaagcuguu cacgggcgag     1920

auccagaucg agcacaucca cccguggucc cgcagccuga acgacuccuu cgccaacaag     1980

acccucugcu acgccgacga gaaccggaag aagggggaca agacccccua cgaguucuac     2040

ggcaacgacg agagcaacug guccgccauc aaggagcgcg cgcugaagcu guucagcgac     2100

accaaggagu accccaacgc cuaccagaag uucaagcggu ucguccagca caaguucgac     2160

gacgacuuca gcucccgcca gcucaacgac acgcgcuaca ucagcaagga ggccaagaac     2220

uaccugucca agaucugcaa gaagguggcc gugagccccg gccaggccac cuccaaccug     2280

cggcagaagu gggggaugaa caacauccuc aacgacgaga acgagaagac ccgcgaggac     2340

caccggcacc acgcgaucga cgcccugguc auggccugca ccaagaucag cuacgugcag     2400

gagcugucca aguggaaccg cuacaaccgg aacgccgagc ucaagaaguu cccgcugccc     2460

ugggagacgu ucuggagcga cgcgaaccgc gccguggaca agauccugau cucccacaag     2520

aaggucagca acgacaucac cggccggacc cacaucuccg agaagaacgg gaagaaguac     2580

aagaacgugg gcguggccgc ccgcgggcag cuccacaagg agaccgucua cggcaagcgg     2640

acguucaacg gcgaggaggc cuuccacgug cgcaagacca ucgacagccu gaccaccgag     2700

aagcagcugg agaagguggu cgacgagucc aucaagcucc ugaucaagaa gcgggugcag     2760

gagcucgggg gcuucgugaa gggggcgauc cccgccaaca ccuucuucgu cguggacgag     2820

aacggcauca agcagcccca gaucuuccug ccgaacaaga acggggcccc cgugcccguc     2880

cugaaggugc gcaugaagga gaacaucggc ggcgccgaga agcugaagga gaacgugaac     2940

caguggguca acccccggaa caaccaccac gugcucaucu acaaggacga gaaggggaac     3000

cugaaggagg agguggucac guucuggacc gugguggagc gcaagcggac cggccagagc     3060

gccuaccagc ugccguucga cgggaaggag aucgucacca cgcuccacau caacgacaug     3120

uuccugcugg gccucaacga ggaggagauc aacugggaga accccgacua cgaggugcug     3180

aaggagcacc uguaccgcgu gcagaaguuc accuccgggg acuacuacuu ccggaucgcg     3240

aagaccagcu ccauccagaa cgccaucgag aagcagcaga ucaacagcuu cggcuucggc     3300

aagaacgggu ggagcaccca caaccccauc aaggucaaga ucuccgugag cggcaagauc     3360

uccaaggugu ga                                                         3372


<210>  1639
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1639
augaacggcc uggugcucgg gcuggacauc ggcaucggga gcgucggcgu ggggauccug       60

aacaaggaca ccggcgagau cauccacacc aacucccgca ucuuccccgc cgccacggcg      120

gacaacaacg uggagcggcg caacagccgg caggcccgcc ggcucaaccg ccggaagaag      180

caccgcggcg cccggcugca cgaccuguuc gaggacuacg accuccugau cgacuucucc      240

aaggucagca ucaaccugaa cccguaccgc cuccgggugc aggggcugga caacccccug      300

accaccgagg agcuguucau cgcccucaag aacaucguga agcgccgggg caucuccuac      360

cuggacgacg ccagcgagaa cgggggcgcg gucuccagcg acuacgggaa ggccguggag      420

gagaaccgca agcuccuggc cgagcagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc aggugcgcgg ggacuucacg gucgaggaga agggcgacaa gcaccggcuc      540

aucaacgugu ucuccaccag cgccuaccgc aaggaggcgg agcggauccu gcgcaagcag      600

caggaguuca acaaccagau caccgacaag uucaucgagg acuaccugaa gauccucacc      660

gagaagcgga aguacuacca cgggcccggc aacgagaagu cccgcacgga cuacggccgc      720

uuccggaccg acgggaccac ccuggacaac aucuucggca uccugaucgg gaagugcacg      780

uucuacccga acgaguaccg cgccagcaag gccuccuaca ccgcccagga guucaaccuc      840

cugaacgacc ugaacaaccu caccgugccc accgagacga agaagcugag cgaggagcag      900

aagaagacca ucgucgagua cgccaagucc gcgaagaccc ugggcgccag cacccuccug      960

aaguacaucg ccaagaugau cgacgccucc guggaccaga uccgggggua ccgcguggac     1020

gucaacaaca agcccgagau gcacacguuc gagguguacc ggaagaugca gagccuggag     1080

accauccccg ugggcgaguu cucccgcgag guccucgacg agcuggccca cauccugacc     1140

cucaacaccg agcgggaggg caucgaggag gcgaucaaca cgaagcugau ggacguguuc     1200

agccaggacc agauccugga gcucgugcag uuccgcaaga acaacagcuc ccuguucagc     1260

aagggguggc acaacuucuc ccugaagcuc augauggagc ugaucccgga gcuguacgag     1320

accagcgagg agcagauggc cauccucacc cggcugggca agcagaaguu caaggagacc     1380

uccaagcgca cgaaguacau cgacgagaag gaggucaccg aggagaucua caaccccgug     1440

guggccaaga gcguccggca ggccaucaag aucaucaacg aggccaccaa gaaguacggg     1500

aucuucgaca acaucgugau cgagauggcg cgcgagaaca acgaggagga cgccaagaag     1560

gacuacauca agcggcagaa ggccaaccac gacgagaaga acgccgccau ggagaaggcg     1620

gccuuccagu acaacggcaa gaaggagcug cccgacucca ucuuccacgg gcacaaggag     1680

cucgccacca agauccgccu guggcaccag cagggcgaga agugccugua cacgggcaag     1740

aacaucccca ucagcgaccu gauccacaac caguacaagu acgagaucga ccacauccuc     1800

ccgcuguccc ugagcuucga cgacucccuc agcaacaagg uccuggugcu ggccaccgcg     1860

aaccaggaga aggggcagcg gacccccuuc caggcccucg acuccaugga cgacgccugg     1920

agcuaccgcg aguucaaguc cuacgugaag gacagcaagc ugcugggcaa caagaagaag     1980

gacuaccucc ugaccgagga ggacaucucc aagaucgagg ucaagcagaa guucaucgag     2040

cggaaccugg uggacacgcg cuacagcucc cggguggucc ucaacacccu gcaggacuuc     2100

uacaaggccc acaaguucga caccaccauc agcguggugc gcgggcaguu caccagccag     2160

cugcgccgga aguggggccu cgagaagucc cgcgagacgu accaccacca cgccgucgac     2220

gcgcugauca ucgccgccag cucccagcug cggcucugga agaagcaggc caacccccug     2280

aucagcuaca aggaggacca guucguggac uccgagaccg gggagaucau cagccugacc     2340

gacgacaagu acaaggagcu cguguucaag gcccccuacg cgcacuucgu cgacacccug     2400

uccagcaaga aguucgagga cuccauccug uucagcuacc agguggacuc caaguacaac     2460

cgcaagauca gcgacgccac gaucuacgcc acccggaagg ugaagcucga caaggacaag     2520

uccgaggaga ccuacguccu gggcaagauc aaggacaucu acagccaggc cggcuacgac     2580

gccuucauca agaucuacaa caaggacaag uccaaguucc ugauguacca caaggacccg     2640

cagaccuucg agaaggugau cgaggagauc cuccgcacgu accccagcaa ggaguucaac     2700

gagaagaaca aggaggugcc cugcaacccc uucgagaagu accggcagga gaacgggccg     2760

auccgcaagu acuccaagaa gggcaacggg cccgagauca agagccugaa guacuacgac     2820

aagaacaucc ccgagagcuu caucaacauc acccccucca acagcaagaa caaggucauc     2880

cugcggcagc ucaacccgug gcgcaccgac guguacuuca accaccagac cgagaaguac     2940

gagcugcugg gccucaagua cgcggaccug caguucgaga agaagacggg caccuacucc     3000

aucaccaucg acaaguacaa cgagaucaag gagaaggagg gggugggcga gcagagcgag     3060

uucaaguuca cccuguacaa gaacgaccuc cugcugauca aggacacgaa gaacaacgag     3120

aagcagaucu uccgguuccu cucccgcacc augcccaacg ucaagcacua cguggagcug     3180

aagcccuacg acaaggccaa guucgacggg aaccagcccc ugaucaccau cuucggcaac     3240

guggccaagg gcgggcagug ccucaagggc cugaacaaga gcaacaucuc caucuucaag     3300

gucaagaccg acgugcuggg gaacaagcac uucaucacga aggagggcga gaagccgaag     3360

cucaaguucu ga                                                         3372


<210>  1640
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1640
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

aacaaggaga ccggggagau cauccacgug aacucccgca ucuuccccgc cgcgaccgcc      120

gacagcaacg ucgagcggcg cggcuuccgg cagggccgcc ggcucgggcg ccggaagaag      180

caccgcuccg cccggcugaa cgaccuguuc gaggaguucg gcuucaucac ggacuucagc      240

gccgugccgc ucaaccugaa ccccuacgcc cugcgcguga aggggcucuc cgaggagcug      300

accaacgagg agcuguucau cgcgcucaag aacaucauca agcggcgcgg caucagcuac      360

cuggacgacg ccuccgagga cggggagacc gccagcaacg aguacggcaa ggccgucgag      420

gagaaccgga agcugcucgc ggacaagacc cccggccaga uccagcugga gcgcuucgag      480

aaguacgggc agguccgggg cgacuucacg gugguggaga acggggagaa ccaccgccug      540

aucaacgugu ucuccaccag cgccuacaag aaggaggccg agcggauccu ccgccggcag      600

caggaguuca acgugcgcau cuccgacgag uucaucgagg ccuaccugac cauccugacc      660

ggcaagcgga aguacuacca cggccccggg aacgagaagu cccgcacgga cuacggccgc      720

uuccggaccg acgggaccac ccucgacaac aucuucggca uccugaucgg gaagugcacg      780

uucuacccgg acgaguaccg cgccgcgaag gccagcuaca ccgcccagga guucaaccug      840

cucaacgacc ugaacaaccu gaccgucccc accgagacga agaagcuccg gcccgagcag      900

aagcgccaga ucguggagua cgcccggacc gccaagaccc ugggcacccc cacgcugcuc      960

aaguacaucg cgaagcuggu ggacggcagc aucgacgaca ucaaggggua ccgcaucgac     1020

aaguccgaca agccggagau gcacaccuuc gacgccuacc ggaagaugcg cacccuggag     1080

cucgucgacg uggacauccu gagccgggag acccuggacg accucgccua cauccugacg     1140

cugaacaccg aguccgaggg cauccucgag gcccugaaca gcaagaugcc cgggaccuuc     1200

accaaggagc agaucgacga gcugauccag uuccgcaaga agaacuccgc cguguucggc     1260

aagggguggc acaacuucag ccucaagcug augaacgagc ugauccccga gcucuacgag     1320

acgagcgagg agcagaugac cauccugacc cggcugggca agcagcgcuc ccgggagauc     1380

agcaagcgca ccaaguacau cgacgagaag gagcucacgg acgagaucua caaccccguc     1440

guggcgaagu ccgugcggca ggccaucaag aucaucaacc uggccaccaa gaaguacggc     1500

aucuucgaca acaucgucau cgagauggcc cgcgagagca acgaggacga cgagaagaag     1560

gccauccaga acgugcagaa ggcgaacgag gacgagaaga aggccgccau ggagaaggcc     1620

gcggaccugu acaacgggaa gaaggagcug ccggacucca ucuuccacgg ccacaaggag     1680

cucgccacca agauccggcu guggcaccag cagggggagc gcugccugua caccggcaag     1740

aacaucagca uccacgaccu cauccacaac ccccaccagu acgagaucga ccacauccug     1800

ccccuguccc ucagcuucga cgacgggcug gccaacaagg ugcugguccu cgccacggcc     1860

aaccaggaga agggccagcg gacccccuuc caggcgcugg acuccaugga cgacgccugg     1920

agcuacaucg aguucaagca guacgugcgc aacuccaaga gccuguccaa caagaagaag     1980

gacuaccucc ugaccgagga ggacaucagc aagaucgagg ucaagcagaa guucaucgag     2040

cgcaaccugg uggacacccg guacuccagc cgcguggucc ucaacacccu gcaggaguuc     2100

uacaagacga acgacuucga caccaagauc uccguggugc ggggccaguu caccagccag     2160

cugcgccgga aguggaagau cgagaagucc cgcgacaccu accaccacca cgccgucgac     2220

gcccucauca ucgccgcgag cagccagcug cggcugugga agaagcagaa caacccgcuc     2280

aucuccuacc gcgaggggca gcugguggac cccgagacgg gcgagauccu gagccucacc     2340

gacgacgagu acaaggagcu ggugcugcgg ccgcccuacg acuacuucgu cgacacccuc     2400

aaguccaaga gcuucgagga cuccauccug uucagcuacc agguggacuc caaguacaac     2460

cgcaagauca gcgacgccac caucuacggg acgcggaagg cccagcuggg caaggacaag     2520

caggaggaga ccuacgugcu cggcaagauc aaggacaucu acucccagaa gggguacgag     2580

gacuucauca agcgcuacaa gaaggacacc acccaguucc ugauguacca caaggacccc     2640

cagacguucg ccaaggucau cgaggagauc cugaagaccu accccgacaa ggagcucaac     2700

gagaagggca aggagauccc cugcaacccc uucgagaagu accggcagga gaacgggccg     2760

auccgcaagu acagcaagaa gggcaagggg cccgagauca agucccugaa guacuacgac     2820

aacaagcugg gcaaccacau cgacaucacc cccgugaaca gccagaacca ggugguccuc     2880

cagucccuga agcccuggcg gaccgacgug uacuucaacc cgcagacgag caaguacgag     2940

cugaugggcc ucaaguacuc cgaccugcgc uucgagaagg ggagcggcuc cuacgggauc     3000

agccccgaga aguacaacaa ggugaaggcc aaggagggcg ucgacgagga cagcgaguuc     3060

aaguucaccc uguacaagaa cgaccucauc cugaucaagg acaccgagac cggggagcag     3120

cagcuguucc gguacggcuc ccgcaacgac acgagcaagc acuacgugga gcucaagccc     3180

uacgagaagg cgaaguucga gggcaaccag cagcugauga accugcucgg gaccguggcc     3240

aagggcgggc agugccugaa gggcaucaac aagccgaacc uguccaucua caaggucaag     3300

accgacgugc ucgggaacaa guacuucauc aagaaggagg gcgaccagcc ccagcugaac     3360

uucaagaaga agaucuga                                                   3378


<210>  1641
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1641
augaccagca agcaggagaa ccugggcgcc cucaccuucg ggcuggacau cggcaucgcc       60

uccguggggu gggcgguccu gggcgagaac cgcaucaucg accucggggu gcggugcuuc      120

gacaaggccg agacggccaa ggagggcgag agccugaacc uggcccgccg gauggcccgc      180

cuccugcggc gccggcugcg ccggcgcgcg uggcggcuca ccaagcuggc ccgccugcuc      240

aagcgggagg gccugaucgc cgacgccaac cuguucaaga acaagaucga gaagaacacc      300

cccgugccca aguccagcug gcagcuccgc gucgacgcgc uggaccggaa gcugaacgcc      360

gaggaguggg cccgcgugau cuaccaccuc ugcaagcacc ggggguucca cuggaucucc      420

cgcgccgagg agaaggccgc ggagagcgac uccaagggcg agagcgggaa ggugaagcag      480

ggccuggccg ggaccaagaa gcugaugcag gagaagaacu accggacggc cgccgagaug      540

guccucgccg aguuccccga ggcgcagcgc aacaagcagg gcgacuacuc caaggcccug      600

agccggaucc ugcucggcga cgagcugaag acccuguucg agcaccagcg caagcucgac      660

ucccccuucg ccaccgagaa gcuggaggcc gccauccugg ggaacggcga ccgcaagagc      720

ggccucuucu gggcgcagaa ccccgcccug gccggguccg accugcucaa gaugcugggc      780

uacugcaccu ucgagaagac ggaguaccgg gccccgaagg ccagcuucac cgcggagcgc      840

cacguguggc ugacccggcu caacaaccug cgcaucgugg ucgacgggac cacgcggccc      900

cugaacgagg ccgagcgccg gaucgcccuc cugaugcccu accagcaggc cggcgacuuc      960

accuacaagc agcugcgcac cgcgcucacc aaggccgggu uccugcccga guccuuccgg     1020

uacacgggcc ugagcuacgc cuccgggcag aaggccggcg agaagaccaa ggacccggag     1080

agcgccgcgc ucgugaaguu ccccgccugg caggagcugc gcaagacccu ggagaagaag     1140

gagcucgccu ccgaguggca gaagaucagc accgacgccc uggacggcaa gccccagcug     1200

cuggaccaga ucgccugggu gcucagcguc uacaaggacg acgacgaggu gaaggcggag     1260

cuguccaagc ugaaccuccc caacgccgag aagaugaucg acgcccugcu ggacauccgg     1320

uucgacaagu ucagcaaccu cucccugaag gcccugugca agaucgugcc gcucauggag     1380

caggggcagc gcuacgacga ggccaucgcg aacauccccg aguacggcca ccacagccag     1440

cugcacaagg ucggggccgg cgagcacaag uaccugccgc ccuucuacaa ggagcgggac     1500

aagacggggc gcaugguguu caacgacgac auggacaucc cccggaaccc cgugguccuc     1560

cgcgcccuga accaggcccg gaagguggug aacgcgcuga ucaagaagua cgacagcccc     1620

cacgaggucc acaucgagau ggcccgcgac cucucccggc cccuggacga gcgccgcgag     1680

guggagaagc ugcagaagga guuccgggac aacaacgaga agggcaaggc ccaguucgcc     1740

caggacuuca acaucguggg cgccgucaag gggaaggagu ucgagaagua ccagcucuac     1800

cgcgagcagc agggcaagug cgcguacucc aucgagccga uggacaucca ccggcuguuc     1860

gagcaggggu acguggagau cgaccacgcc cugcccuaca gccgcuccuu cgacgacagc     1920

aagaacaacc ggguccucgu gcuggccaag gagaaccgcg acaagggcaa ccggaccccc     1980

uucgaguacc ucgggggcaa ggacaacucc gagcccuggc gccaguucgu ggccuucguc     2040

gagggcaaca aggccuaccg gcuggcgaag cgcagccggc ugcugcgcaa ggacuucggg     2100

gacaaggagg ccaaggaguu ccgggagcgc aaccucaacg acacccggua caucugcaag     2160

uucuucaaga acuacgugga gcaguaccug cagcugcacg aggacuccga ggccaagcgc     2220

ugcguggucc ucagcggcca gaugaccucc uuccugcggg cccgcugggg gcuggugaag     2280

gugcggggcg acagcgaccg ccaccacgcc cucgacgcgg ccgucguggc cgccugcucc     2340

cacggcaugg ugaagcggcu gagcgacuac ucccgccgca aggagcugga gcagguccgg     2400

gagggguucg uggacgugga cacgggcgag aucgucaacc cggcgauguu ccagcagguc     2460

caggagcacu uccccaaccc cuggccccac uuccgcgacg aggugaagcu ccggcugagc     2520

auggacgacc cggcccugcu ccgcagcgag auggagaagu ucgggaccua cacccccgag     2580

gcccugcagg cccugcaccc ccucuucgug ucccgggccc cgcagcgccg gaacagcggc     2640

gcggcccaca aggacaccau cuacgcccag cccaagcgcc ugguggccca gggcggggug     2700

acgcagaagg ucgcccugac cucccucacc cugaaggacc uggacaagcu caucgacccc     2760

caccggaacg acaagcugua caccgcgauc cgcgcccggc uggagcagca cggcgggaag     2820

ggcgacaagg ccuuccccag cuccaacccg cuccgcaagc ccgacagcga ggggaacccc     2880

acgggcccca ucgugcgcgg ggugaccaug gucaucgaca agcuguccgg caucccgauc     2940

cggggcggga ucgccaagaa cgacagcaug gugcgggugg acgucuucac caaggccaac     3000

aaguuccacc uggugcccgu guacguccac cacaccgugg cgaaggcccu ccccaaccgc     3060

gccguggucg ccuucaagga cgaggacgag uggacgcuga ucgacgacuc cuucgacuuc     3120

ugcuucagcc ugcaccccaa cgaccucguc caggugcagc agaagggcaa ggccgggaug     3180

aucggcuacu acuccagcug ccaccggggg accggcaaca ucaaccugug ggcgcacgac     3240

cgcaaccucc aggugggcaa ggacgggaug aucgagggca ucggggugaa gaccgcccug     3300

ucccuggaga aguucaacgu ggacguccug ggcaaccggu acccggcccc gcccgagaag     3360

cgccgcgacc ucgccuga                                                   3378


<210>  1642
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1642
augaccgacc gcaucagccu gggccucgac aucggggugg ccuccgucgg cuucagcgug       60

cuggacaucg acaaggggaa ggugaucgag cugggcgccc gccucuucaa cgcgaccguc      120

gccgccggga accaggaccg gcgggacaug cgcggcuccc ggcgccugcu gaaccggaac      180

aagcagcgcc ggaaggacgu gagcaagcug uuccaggagu acggccuccu ggacaacuuc      240

gaccgcgaca acuuccacac ggccuucaac aacaacugga accccuacga gcuccgggug      300

aaggggcuga ccaagcagcu gaacaaggag gagcucgccg acucccugua ccagaucauc      360

aagcgccggg gcaucagcua cgcgcugaag gacgccgacg ccaacgagga cgggaccgac      420

uacuccagcu cccucaagau caacagccag gagcugaucg agaagacccc ggcccagauc      480

cagcugcagc gccucaacga cuacggcaag guccggggga aggugaucgu gggcgacgac      540

aucgacgacc agaagguccu gcugaacgug uuccccacga acgcguacga gaaggaggcc      600

cgccagauca ucgccaccca gcagcaguuc uaccccgaga uccucaccgg cgaguucgug      660

aaggaguacu gccagauccu gacccggaag cgcgacuacu ucgucgggcc cggcaacgag      720

aagucccgga cggacuacgg gaucuacaag accgacggcc gcacccugga caaccucuuc      780

gaggagcuga ucggccacga caagaucuac ccggacgagc ugcgggccag cgccgcgucc      840

uacaccgccc agcuguucaa cgugcucaac gaccugaaca accuccgcau ccugagcuac      900

gaggacggga agcugacgca gaucgacaag gagaagauca ucgccgagau caagaacaac      960

accaccgugg ucaacaugcu caacgugauc aagaagguga ccggcugcca gaaggacgac     1020

aucaaggggu uccgccugaa cgagaaggac aagcccgaga ucuccagcau gcccaucuac     1080

cggaaggccc acaaggacuu ccugaagguc ggcaucgaca ucacggacug gcccaucgac     1140

uucaucgacc gccucuccuu cauccugacc cugaacaccg agaacgggga gauccggaag     1200

cagaucaaca agcgccucgu gccggacuuc gacuuccuga acgacgaccu ggugcagcuc     1260

aucaucgaca acaaggacag cuucgacauc aagaccaaca acaaguggca ccgguucucc     1320

cugaagacga ugaacaagcu gauccccacc augaucgagc gccccgucga gcagaugacc     1380

cuccugaccg agaugggccu gaucaagaag gacacgaagc gguucgaggg caacaaguac     1440

cuccccuaca aggagaucgc caacgacauc uucaacccgg uggcgagcaa gagcgugcgc     1500

gaggcccuga agaucgucaa cgccgugcug aagaaguacg ggcacaucga guacaucguc     1560

aucgagaugc cccgggacaa gaaccuggac gaggagcgca agcagaucgc cgacuuccag     1620

aagaagaaca agaagaucaa ggacgccgcg uucaacgccu ucgugaaggc cgugggcucc     1680

aagaagaacg ucaagaucgc ccucagcaag aaccggaagc ugcagauggg gauccgccug     1740

uggcaccagc agcagggcau cgaccccuac aacggggagg agaucaacgc caacgaccuc     1800

aucuccaacc ccgacaaguu cgagaucgac cacaucaucc cgcagagcau cuccuucgac     1860

gacggcauca acaacaagac ccugugcuuc gcgagcauga accaggugaa gggccagaag     1920

acccccuacg aguuccugaa cgaggggcac ggccaggggu acgccaaguu caaggccauc     1980

guguccaaga acaagaacuu cagcaaggcc aagcgggaca acuaccucuu cgaggagaac     2040

gucuccaaca ucgagacccg caagcgguuc cugagccgca accuggugga cacgcgguac     2100

uccagccgcg ugguccucaa cucccugcag gaguucuucc acgagaaguc cgacgacacc     2160

aaggugaccg ugauccgggg caaguucacc agcaacaugc gcaagcacug gcacaucgac     2220

aagacgcgcg acaccuucca ccaccacgcg aucgacgcca gcaucaucgc cgccaccccc     2280

uuccugcgga uguggaagaa gggguccacc aucuucccca cgaagaucaa cgagaccagc     2340

aucgacaucg agaccggcga gauccucgac gacaagaacu ucgacaaggu cauguacgag     2400

gagccguacu ccggcuucau cagcgagauc augaacgccg acgaccgcau caaguucagc     2460

caccaggugg acaagaagau gaaccggaag guguccgacg cgaccaucua cagcacccgc     2520

aucgggaagc uguccaagga caagaaggac gccgaguacg ucguggccaa ggugaaggac     2580

aucuacagcg ucgacggcua cgagaacuuc aagaaggugu acaagaagga caagacgaag     2640

uuccugcucu acaaguacga cccccggacc uucuccgagc uggagaagau cgugaucgac     2700

uaccccgaca agaucgagaa gguccagacc aacgggaaga ucaaggccgu ggacaucagc     2760

cccuucgagc uguaccgccg ggacaacggc auggugcgca aguacuccaa gaagaccggc     2820

ccggccauca agcagcucaa guaccuggac aagaagcugg ggagccacau cgacaucacg     2880

cccaagaacg cgaacaacaa gcacgucgug cuccaguccc ugaagcccug gcggaccgac     2940

guguaccuga accacgagac cggcgaguac gagaucaugg ggaucaagua cagcgaccuc     3000

aaguucaaca agaacgaggg cuacgggauc aagaaggaca aguaccugaa gaucaagaac     3060

aucgagggcg ucuccgagaa cagcgaguuc auguucuccc uguaccgcaa ggaccggauc     3120

aaggugcagg acauggagac caacgagagc guggagcucc uguucugguc ccgcaacuuc     3180

agcaacaaga aguacgccga gcugaagccc aucucccaga ucgacaacga caagguccuc     3240

ccgaucuacg gccgggggcg ccugaucaag cggcugaucc ccaagaacug caagaucugg     3300

aaggugaaca cgagcauccu cggcgacacc uacuaccugg agaaggagag caacuacccc     3360

cagaagaucc uggacuga                                                   3378


<210>  1643
<211>  3381
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1643
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

aacaaggaga ccggggagau cauccacgcc aacucccgca ucuuccccgc ggccaccgcc      120

gacagcaacg uggagcggcg cggcuuccgg cagggccgcc ggcucgggcg ccggaagaag      180

caccgcuccg cccggcugaa caaccuguuc gaggaguucg gcuucaucac ggacuucagc      240

gccaucccgc ucaaccugaa ccccuacgcg cugcgcguca aggggcucuc cgaggagcug      300

accaacgagg agcuguucau cgcccucaag aacaucauca agcggcgcgg caucagcuac      360

cuggacgacg ccuccgagga cggggagacc gccagcaacg aguacggcaa ggcgguggag      420

gagaaccgga agcugcucgc cgacaagacc cccggccaga uccagcugga gcgcuucgag      480

aaguacgggc agguccgggg cgacuucacg gugguggaga acggggagaa ccaccgccug      540

aucaacgugu ucuccaccag cgccuacaag aaggaggccg agcggauccu ccgccggcag      600

caggaguuca acguccgcau cuccgacgag uucaucgagg ccuaccugac cauccugacc      660

ggcaagcgga aguacuacca cggccccggg aacgagaagu cccgcacgga cuacggccgc      720

uuccggaccg acgggaccac ccucgacaac aucuucggca uccugaucgg gaagugcacg      780

uucuacccgg acgaguaccg cgcggccaag gccagcuaca ccgcccagga guucaaccug      840

cucaacgacc ugaacaaccu gaccgugccc accgagacga agaagcucag ccccgagcag      900

aagcggcaga ucguggagua cgcccgcacc gcgaagaccc ugggcacccc cacgcugcuc      960

aaguacaucg ccaagcuggu cgacggcucc aucgacgaca ucaaggggua ccggaucgac     1020

aagagcgaca agccggagau gaugcacacc uucgacgccu accgcaagau gcggacccug     1080

gaccucguga acaucgacgc ccugucccgc gagacccugg acgaccucgc ccacauccug     1140

acgcugaaca ccgagagcga gggcauccuc gaggcgcuga acuccaagau gcccagcacc     1200

uucaccaagg agcagaucga cgagcugauc caguuccgga agaagaacag cgccguguuc     1260

gggaagggcu ggcacaacuu cucccucaag cugaugaacg agcugaucag cgagcucuac     1320

gagacguccg aggagcagau gaccauccug acccgccugg ggaagcagcg gagccgcgag     1380

aucuccaagc ggaccaagua caucgacgag aaggagcuca cggaggagau cuacaacccc     1440

gucguggcca agagcgugcg ccaggccauc aagaucauca acgaggccac caagcgguac     1500

ggcaucuucg acaacaucgu caucgagaug gcgcgcgaga acaacgagga ggacgccaag     1560

aaggacuaca ucaagcggca gaaggccaac caggacgaga agaacgccuc cauggagaag     1620

gcggccuucc aguacaacgg caagaaggag cugcccgaca gcaucuucca cgggcacaag     1680

gagcuggcca ccaagauccg ccuguggcac cagcagggcg agcggugccu cuacaccggg     1740

aagaacaucu ccauccgcga ccugauccac aacccgcacc aguacgagau cgaccacauc     1800

cugccccuca gccuguccuu cgacgacggc cuggccaaca aggugcucgu gcuggccacg     1860

gcgaaccagg agaaggggca gcgcaccccc uuccaggcca ucgacagcau ggacgacgcc     1920

ugguccuacc gggaguucaa gcaguacguc cgcaacagca agucccugag caacaagaag     1980

aaggacuacc uccugaccga ggaggacauc uccaagaucg aggugaagca gaaguucauc     2040

gagcggaacc ugguggacac ccgcuacagc ucccgggucg ugcucaacac ccugcaggag     2100

uucuacaaga cgaacgacuu cgacaccaag aucagcgucg ugcgcggcca guucaccagc     2160

cagcugcggc gcaaguggaa gaucgagaag ucccgggaca ccuaccacca ccacgccgug     2220

gacgcccuca ucaucgcggc cagcucccag cugcgccugu ggaagaagca gaacaacccc     2280

cucaucagcu acaaggaggg ccaguucguc gacccggaga cgggggagau ccugucccug     2340

accgacgacg aguacaagga gcucguguuc cggccgcccu acgacuacuu cguggacacc     2400

cucaagagca aguccuucga ggacagcauc cuguucuccu accaggucga cagcaaguac     2460

aaccgcaaga ucuccgacgc caccaucuac ggcacgcgga aggcccagcu cgggaaggac     2520

aagcaggagg agaccuacgu gcugggcaag aucaaggaca ucuacagcca gaagggcuac     2580

gaggacuuca ucaagcgcua caacaaggac gagacccagu uccugaucua ccacaaggac     2640

ccccagaccu ucgagaaggu gaucgaggag auccucaaga cguaccccga caaggagcug     2700

aacgagaagg ggaaggagau ccccugcaac cccuucgaga aguaccggca ggagaacggc     2760

cccauccgca aguacuccaa gaaggggaag ggcccggaga ucaagauccu gaaguacuac     2820

gacaacaagc ucgggaacca caucgacauc acccccguca acagccagaa ccagguggug     2880

cugcaguccc ugaagcccug gcggaccgac gucuacuuca acccccgcac cagcaaguac     2940

gagcucaugg gccugaagua cuccgaccug cgguucgaga agggcagcgg gagcuacggc     3000

aucuccccgg agaaguacaa caaggugaag gccaaggagg gggugaacga ggacagcgag     3060

uucaaguuca cgcucuacaa gaacgaccug auccugauca aggacaccga gaccggcgag     3120

cagcagcugu uccgcuacgg gucccggaac gacaccagca agcacuacgu cgagcugaag     3180

cccuacgaga aggcgaaguu cgagggcaac cagcagcuga ugaaccuccu gggcacggug     3240

gccaagggcg ggcagugccu gaaggggauc aacaagccca accucuccau cuacaaggug     3300

aagaccgacg uccugggcaa caaguacuuc aucaagaagg agggggacca gccgcagcug     3360

aacuucaaga agaaguucug a                                               3381


<210>  1644
<211>  3381
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1644
auggccagca agcacgagaa ccugggcgcc cucaccuucg ggcuggacau cggcaucgcg       60

uccguggggu gggccguccu gggcgagaac cgcaucaucg accucggggu gcggugcuuc      120

gacaaggccg agaccgccaa ggagggcgag agccugaacc uggcccgccg gacggcgcgc      180

cuccugcggc gccggcugcg ccggcgcgcc uggcggcuca ccaagcuggc ccgccugcuc      240

aagcgggagg gccugaucgc cgacaccaac guguuccaga agcccuucaa ccugaaggac      300

gcgaacgaga aggggcucuc cuucuggcag cugcgcgucg acgcccugga ccggaagcuc      360

agcgccgagg agugggcccg cgugaucuac caccugugca agcaccgggg cuuccacugg      420

aucucccgcg ccgaggagaa ggcggccgag ggggacagca aguccgaggg cgggaaggug      480

aagcagggcc uggccggcac caagaagcuc augcaggaga agaacuaccg gacggccgcc      540

gagauggucc ugaccgaguu ccccgaggcg cagcgcaaca agcaggggga cuacagcaag      600

gcccuguccc ggauccuccu gggcgacgag augacccagc uguucaagca ccagcgcgag      660

cucgggaacc cccacgccac cgagaagcug gaggccgcca uccugggcaa cggcgaccac      720

aagagcggcc ucuucugggc gcagaacccc gcccuggccg ggaacgaccu gcucaagaug      780

cuggggcacu gcacguucga gaaggccgag uaccgcgugc ccaaggccuc cuucaccgcg      840

gagcggcacg uguggcugac ccgccucaac aaccugcgga ucgucgugga cggcaccacg      900

cgcccgcuga acgaggccga gcggcaccuc gcccugcuga ugcccuacca gcaggccggg      960

gaccucaccu acaagcagcu gcgcaccgcg cugaccaagg ccggcuaccu ccccgagagc     1020

uuccgguaca cgggccuggc cuacgccucc gggcagaagg ccgacgagaa ggcgaaggac     1080

cccgagagcg ccccgcuggu gaagcucccc gccuggcacg agcugcgcaa gacccuggag     1140

aagaaggagc uggccuccga guggcagaag aucagcuccg acgcccucga cggcaagccc     1200

cagcugcugg accagaucgc guggguccuc agcguguaca aggacgacac cgagguggag     1260

gccgagcugu gcaagcugaa ccuccccaac gccgagaaga ugaucgacgc ccugcuggac     1320

auccgguucg acaaguucuc caaccucagc cugaaggccc ugcgccagau cgucccgcuc     1380

auggagcagg ggcagcggua cgacgaggcg guggccaaca uccccgagua cggccaccac     1440

agccagcugc acgagguggg ggccggcgag cacaaguacc ugccgcccuu cuacaaggag     1500

cgcgacaaga ccggccggau ggucuucaac gacgacaugg acaucccccg caaccccgug     1560

gugcuccggg cccugaacca ggcgcgcaag gucgugaacg cccugaucaa ggaguacggg     1620

agcccccacg aggugcacau cgagauggcc cgggaccucu cccgcccccu ggacgagcgg     1680

cgcgacgucg agaagcugca gaaggaguuc cgcgaccgga acgagaagga caaggcccuc     1740

uucgcccagg acuucaacau cgugggcgcg gucaagggga aggaguucga gaaguaccag     1800

cuguaccgcg agcagcaggg caagugcgcc uacuccaucg agccgaugga cauccaccgg     1860

cuguucgagc agggguacgu ggagaucgac cacgcccucc ccuacagccg cuccuucgac     1920

gacagcaaga acaaccgggu gcugguccug gccaaggaga accgcgacaa gggcaaccgg     1980

acgcccuucg aguaccucgg cgggaaggac aacuccgagc gcuggcggca guucguggcc     2040

uucguggagg gcaacaagag cuaccgccug gcgaagcggu cccgccugcu ccggaaggac     2100

uucgggaagg acgaggccaa gggcuuccgc gagcggaacc ugaacgacac ccgcuacauc     2160

ugccgguucu ucaagaacua cgucgagcag uaccugcagc uccacgagga cagcgacgcc     2220

aagcgcugcg uggugcuguc cggccagaug accagcuucc ugcgggcccg cugggggcuc     2280

gucaaggugc ggggcgacuc cgaccgccac cacgcccugg acggggcgau cguggccgcc     2340

ugcagccacu ccauggucaa gcgccugagc gacuacuccc ggcgcaagga gcucgagcag     2400

guccgggccg gcuucgugga cguggacacc ggggagaucg ugaaccccgc gauguuccag     2460

cagguccagg agcacuuccc gaaccccugg ccccacuucc gcgacgaggu gaagcugcgg     2520

cugaacgugg acgaccccgc ccuccugcac agcgagauga accgcuucgg cacguacucc     2580

ccggaggccc ugaaggcccu ccggccccug uucaucagcc gcgcgcccca gcggcgcaac     2640

agcggcgccg cccacaagga caccaucuac gcccagccgg agcggcuggu ggcccagggc     2700

ggggucaccc agaaggugcc ccucaccucc cugacgcuga aggaccucga caagcugauc     2760

gacccccacc gcaacgagaa gcuguacagc gccauccgga uccgccucga gcagcacggg     2820

ggcaaggggg acaaggcguu ccccgccucc aacccgcugc ggaagcccga cagcgagggc     2880

aaccccaccg gccccaucgu gcgcgggguc accaugguga ucgacaagcu guccggcauc     2940

ccgauccgcg ggggcaucgc caagaacgac agcauggugc gggucgacgu guucaccaag     3000

gccgggaagu uccaccucgu gcccgucuac gugcaccacg ccguggcgaa gguccugccc     3060

aaccgggccg ugguggccua caaggacgag acggagugga cccugaucga cgacggcuuc     3120

gacuucugcu ucuccaugua ccccaacgac cuccugcgca ucaagcugcg caacgccagc     3180

caccucggcu acuacgccuc cugcaaccgg agcaccgggg cgaucaaccu guggucccac     3240

gaccgcaacc agcaggucgg caaggacggg augaucgaga gcaucggcgu gaagaccgcc     3300

cugaacgugg agaagcucaa caucgacguc cuggggaacc gguacccggc cccgcccgag     3360

aagcgccggg accuggccug a                                               3381


<210>  1645
<211>  3384
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1645
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacg ucgagcggcg caucaaccgg cagggccgcc ggcucacccg ccggaagaag      180

caccgccggg ugcgccugaa ccaccuguuc gaggaguccg gccucaucac ggacuucacc      240

aacgugagca ucaaccugaa cccguaccag cugcggguca aggggcucac cgacgagcug      300

uccaacgagg agcuguucau cgcccucaag aacaugguga agcaccgcgg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgucaag      420

gagaacagca agcagcugga gaccaagacg cccgggcaga uccagcucga gcgguaccag      480

aaguacggcc agcugcgcgg cgacuucacc guggaggagg acgggaagaa gcaccggcug      540

aucaacgugu uccccaccuc cgccuaccgc gcggaggccc uccggauccu gcagacccag      600

caggaguuca acccccagau cacggacgag uucaucaaca gcuaccugca gauccucacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggaccga cuacggccgc      720

uacaccacga agaaggacag cgaggacgag uacaucaccc uggacaacau cuucgggauc      780

cugaucggca agugcaccuu cuaccccgag gaguaccggg ccgccaaggc guccuacacc      840

gcccaggagu ucaaccuccu gaacgaccug aacaaccuca cgguccccac cgagaccaag      900

aagcugagcg aggagcagaa gaaccagauc aucaccaacg ugaagaacga gaaggccaug      960

gggcccgcca agcuguucaa guacaucgcc aagcuccugu ccugcgacgu ggcggacauc     1020

aagggcuacc gcaucgacaa gagcgacaag gccgagaucc acacguucga ggccuaccgc     1080

aagaugaaga cccuggccac caucgacauc gagcagaugg agcgggagaa ccucgacaag     1140

cuggccuacg uccugacccu caacacggag aaggagggga uccaggaggc gcuggagcac     1200

gaguucgccg acggcaccuu cucccaggag cagaucgacg agcuggugca guuccgcaag     1260

gccaacagca gcaucuucgg caaggggugg cacuccuuca gcgugaagcu caugauggag     1320

cugaucccgg agcuguacgc caccuccgag gagcagauga ccauccucac gcggcugggc     1380

aagcagaaga ccaccagcuc cagcaacaag accaaguaca ucgacgagaa gcagcugacg     1440

gaggagaucu acaaccccgu cgccgcgaag uccgugcgcc aggccaucaa gaucgugaac     1500

gccgccauca aggaguacgg ggacuucgac aacaucguca ucgagauggc ccgggagacc     1560

aacgaggacg acgagaagaa ggcgauccag aagauccaga aggccaacaa ggacgagaag     1620

gacgccgcca ugcucaaggc ggccaaccag uacaacggca aggccgagcu gccccacagc     1680

guguuccacg ggcacaagca gcuggccacc aagauccgcc ucuggcacca gcagggcgag     1740

cggugccugu acaccggcaa gacgaucucc auccacgacc ugaucaacaa cagcaaccag     1800

uucgagaucg accacauccu gccccucucc aucaccuucg acgacagccu ggccaacaag     1860

gugcuggucu acgcgaccgc caaccaggag aaggggcagc gcaccccgua ccaggcccuc     1920

gacuccaugg acgacgccug gagcuuccgg gagcugaagg ccuucgugcg cgacuccaag     1980

gcgcugagca acaagaagaa ggaguaccuc cugaccgagg aggacaucuc caaguucgac     2040

guccggaaga aguucaucga gcgcaaccug guggacacgc gguacgccag ccgcgugguc     2100

cucaacgccc ugcaggagca cuuccgggug cacaagaccg acaccaaggu guccgucgug     2160

cgcggccagu ucaccagcca gcugcggcgc cacuggggga ucgagaagac gcgggacacc     2220

uaccaccacc acgccgugga cgcccucauc aucgcggcca gcucccagcu gaaccugugg     2280

aagaagcaga agaacacccu cgucagcuac uccgaggacc agcugcugga caucgagacc     2340

ggcgagcuca ucagcgacga cgaguacaag gaguccgugu ucaaggcccc cuaccagcac     2400

uucguggaca cgcucaagag caaggaguuc gaggacucca uccuguucag cuaccagguc     2460

gacuccaagu ucaaccgcaa gaucagcgac gccaccaucu acgcgacccg ccaggccaag     2520

guggggaagg acaagaagga cgagaccuac gugcucggca agaucaagga caucuacucc     2580

cagacgggcu acgacgccuu caucaagauc uacaagaagg acaagagcaa guuccugaug     2640

uaccggcacg acccccagac cuucgagaag gucaucgagc ccauccugga gaacuacccg     2700

aacaaggagc ucaacgagaa ggggaaggag gugcccugca accccuuccu gaaguacaag     2760

gaggaccacg gcuacauccg caaguacucc aagaagggga acggccccga gaucaagagc     2820

cugaaguacu acgacuccaa gcucggcaac cacaucgaca ucaccccgaa gaacagcaac     2880

aacaaggugg uccugcaguc cgugagcccc uggcgggccg acguguacuu caacaagacc     2940

acggggaagu acgagauccu gggccucaag uacgccgacc ugaaguucga gaaggggacc     3000

ggcaccuaca agaucagcga ggagaaguac aacgacauca agaucaagga gggggucgac     3060

uccgacagcg aguucaaguu cacccuguac aagaacgacc uccugcugau caaggacacg     3120

gagaccaagg agcagcagcu guuccgcuuc cugucccgga ccaugcccaa cgugaagcac     3180

uacguggagc ugaagcccua cgacaagcag aaguucgacg acaacgagga gcucaucaag     3240

auccugggca ucgucgcgaa gggcgggcag ugcaagaagg gcgugagcaa gccgaacauc     3300

uccaucuaca agauccgcac cgacgugcug gggaaccagc acaucaucaa gaacgagggc     3360

gacaagccca agcucgacuu cuga                                            3384


<210>  1646
<211>  3384
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1646
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaaggacu ccgggaagau cauccacgcc aacagccgca ucuuccccgc ggccaccgcc      120

gacaacaacg uggagcggcg caccucccgg caggcccgcc ggcucaaccg ccggaagaag      180

caccgcagcg uccggcugca cgaccuguuc gaggaguacg gccuccugac ggacuucucc      240

aaggugagca ucaaccugaa cccguaccag auccgcgugc agggccucga caagcggcug      300

accaacgagg agcuguucau cgcccucaag aacaucguca agcgccgggg gaucuccuac      360

cuggacgacg cgagcgagga cggcggggcc guguccagcg acuacggcaa ggccguggag      420

gagaaccgca agcugcucgc ccagcagacc cccgggcaga uccagcugga ccgguucgag      480

aaguacggcc agguccgcgg cgacuucacc gugguggaga acggggagaa gcggcgccug      540

aucaacgucu ucuccacgag cgcguaccgg aaggaggccg agcgcauccu ccggaagcag      600

caggaguuca acaacaagau caccgagggc uucaucgagg acuaccugac cauccugacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccgcacgga cuacgggcgg      720

uacaccacca agaaggacag ccagggcgag uacaucaccc ucgacaacau cuucgggauc      780

cugaucggca agugcacguu cuaccccgac gaguaccgcg ccuccaaggc cagcuacacc      840

gcccaggagu ucaaccugcu caacgaccug aacaaccuga ccgugccgac cgagacgaag      900

aagcucuccg uggagcagaa gaagaccauc gucgaguacg cgaagaccgc caagacccug      960

ggggccagca cgcugcucaa guacaucgcc aagcugaucg gcguguccgu ggaccagauc     1020

cacggcuacc ggaucgaccc caacgagaag cccgagaugc acaccuucga ggucuaccgc     1080

aagaugcaga gccuggagac cauccgggug gaggagcucc cccgcaaggu gaucgacgag     1140

cuggcccaca uccugacccu caacacggag cgggagggga ucgaggaggc gaucaacgcc     1200

aagcugaagg acaucuucag ccaggaccag guccuggagc ucgugcaguu ccgcaagaac     1260

aacuccagcc uguucuccaa gggcuggcac aacuucagcc ugaagcucau gauggagcug     1320

aucccggagc uguacgagac cuccgaggag cagaugacca uccucacccg gcuggggaag     1380

cagaagucca aggagacgag caagcgcacc aaguacaucg acgagaagga gcugaccgag     1440

gagaucuaca accccguggu cgccaagagc gugcggcagg ccaucaagau caucaacgag     1500

gccaccaagc gcuacggcau cuucgacaac aucgucaucg agauggcgcg ggagaacaac     1560

gaggaggacg ccaagaagga guacaucaag cgccagaagg ccaaccucga cgagaagaac     1620

gccgccaugg agaaggcggc cuuccaguac aacgggaaga aggagcugcc cgacaacgug     1680

uuccacggcc acaaggagcu ggccacgaag auccggcugu ggcaccagca gggcgagaag     1740

ugccucuaca ccgggaagaa cauccccauc uccgaccuga uccagaaccg cuacaaguac     1800

gagaucgacc acauccugcc gcucagccug uccuucgacg acagccuguc caacaaggug     1860

cucguccugg ccaccgcgaa ccaggagaag ggccagcgga cccccuucca ggcccuggac     1920

agcauggacu acgccugguc cuaccgcgag uucaagagcu acgugaagga cuccaagcuc     1980

cugagcaaca agaagaagga cuaccugcuc acggaggagg acaucuccaa gaucgaggug     2040

aagcagaagu ucaucgagcg gaaccugguc gacacccgcu acagcagccg cguggugcug     2100

aacgcccucc aggacuucua caaggagcac caguucgaca ccaccaucuc cgucgugcgg     2160

gggcaguuca ccagccagcu gcgccggaag uggggcaucg agaagucccg cgagacguac     2220

caccaccacg ccguggacgc gcugaucauc gccgccagcu cccagcuccg gcuguggaag     2280

aagcacaaca acccccugau cagcuacaag gaggagcagu ucgucgacuc cgagaccggg     2340

gagaucauca gcauguccga cgacgaguac aaggagcucg uguucaaggc ccccuacgac     2400

cacuucgugg acacccucaa gagcaagaag uucgaggacu ccauccuguu cagcuaccag     2460

gucgacucca aguacaaccg caagaucagc gacgccacca ucuacgcgac gcggaaggcc     2520

aagcuggaca aggacaaguc cgaggagacc uacgugcugg gcaagaucaa ggacaucuac     2580

agccaggccg gcuacgacac cuucaucaag aucuacaaca aggacaaguc ccgcuuccug     2640

auguaccaca aggacccgca gaccuucgag aaggugaucg aggagauccu ccggacguac     2700

cccagcaagg agcugaacga gaagaacaag gagguccccu gcaaccccuu cgagaaguac     2760

cgccaggaga acgggccgau ccggaaguac agccgcaagg gcaacgggcc cgagaucaag     2820

ucccugaagu acuacgacaa caagcucggc aaccacaucg acaucacccc caacgagagc     2880

augaaccagg uggugcugca gucccugaag cccuggcgga ccgacgucuu cuucaaccac     2940

cagaccgaga aguacgagcu caugggccug aaguacagcg accugaacuu cgagaagggg     3000

uccggcaagu acagcaucuc ccucgagaag uacaacagca ucaagaugaa cgagggggug     3060

uccgaggaga gcgaguucaa guucacgcug uacaagaacg accugauccu caucaaggac     3120

gcccugacca aggaggccca gaucuuccgc uacggcuccc ggaacgacac cagcaagcac     3180

uacguggagc ugaagccgua cgacaugucc aaguucgagg gggacgagcg ccucaucaag     3240

guccugggca ccguggcgaa gggcgggcgg ugcaucaagg gccugaacaa gaaggacauc     3300

agcaucuuca aggugaagac ggacguccuc gggaacaagc acuucaucaa gaaggagggc     3360

gaggagccca agcugaaguu cuga                                            3384


<210>  1647
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1647
augaccaagc ugggcaagcc cuacgggauc ggcguggaca ucgggagcaa cuccaucggc       60

uucgccgccg ucgacgagaa cagccaccuc auccgccuga aggggaagac cgugaucggc      120

gcgcggcugu ucgaggaggg caaggccgcc gccaagcgcc gggccgggcg cacgacccgg      180

cgccggcucu cccgcaaccg guggcgccug agcuuccugc gggacuucuu cgagucccac      240

aucaccccga ccgaccccaa cuucuucaug cgccagaagu acagcgagau cucccccaag      300

gacaaggcgc gguacaagua cgagaagcgc cucuucaacg accggacgga cgccgaguuc      360

uaccagcagu acagcaccau guaccaccuc cgcaaccggc ugaugaccga ccccucccgc      420

gccgacgugc gggagaucua cuucgccauc caccacaucc ucaagagccg cggccacuuc      480

cugaccccgg gggacgcgaa ggacuucaac acgaacgacg ucgcccugga cgagaucuuc      540

cccgcccugc aggacgccua cgcccaggug uaccccgacc ugggcaucac cuucgacgag      600

gacaaggcga acgaguucaa gaccauccug cucaacgaga ucgccaccuc caucgacacg      660

cagcgggccc uggugaagcu gcuccuggcc gaggaggacg acaaggacau ccugaagcag      720

cagaagcagg uccucaccga guucgccaag gcgguggugg ggcugaagac caagcugaac      780

cucgcccugg gcaccgaggu cgacagcucc gaggccacgg ccuggaacuu cagccugggc      840

cagcucgacg acaagugggc cgggaucgag uccgcgauga ccgacgaggg caccgagauc      900

cuggaccaga uccgcgaccu guaccgcgcc cggcuccuga acgggaucgu gcccaccggc      960

aagacgcuga gccaggccaa gguggacgac uacacccagc accacgagga ccuccagcug     1020

uucaaggccu accugaagca gcucgaggac gacggcaccg ccaaggcgau ccgccagcug     1080

uacgaccggu acaucgacgg ggacgacgcc gccccguucc ugcgcgagaa cuucgucaag     1140

gcccucacca aggacgugac ggcgcacccc aacaccaagu cccccgagcu gcuggagcgg     1200

cucgcccagc ccgacuucau gcugaagcag cgcaacaagg ccaacggcgc caucccggug     1260

cagaugcagc agcgggagcu ggaccagauc aucaagaacc aggccgucua cuacgacugg     1320

cucgcggccc ccaaccccgu ggagaagcac cgcaagagca ugcccuacca gcuggacgag     1380

cugcucaacu uccggauccc guacuacgug gggccccugg ucaccgccaa ggagcagaag     1440

gccgcccagg gcgggguguu cgcguggaug gugcgcaagg accccgaggg caacaucacc     1500

cccuacaacu ucgacgagaa ggucgaccgg gaggccuccg ccaacacguu cauccagcgc     1560

augaagacca ccgacaccua ccugaucggc gaggacgugc ugccgaagca gagccuccug     1620

uaccagcggu acgaggugcu gaacgagcuc aacaacgucc gcgugaacaa cgagaagcug     1680

agcaucgagc agaagcagca ggucauccgg gagcuguucg agcgccacaa cuccgugacg     1740

aucaagcagu ucgccgagaa ccuccgggcc cacggggacu acgcgcacau ccccgagauc     1800

cgcggccugg ccgacgagaa gcgguuccug agcucccuca gcaccuaccg ccagcugaag     1860

ucccugcucc ccgaggccau cgacgacccc gccaagcagg cggacaucga gaacaucauc     1920

gccuggagca ccguguucga ggacgccgcc aucuucaaga ccaagcugaa ggagaucucc     1980

uggcugggga gcgaggccau cacgaagcuc uccaacaucc gcuaccgggg cugggggcag     2040

uucagccgca aguuccugaa cggccugacc cucggcaacg ggcacaccau cauccaggag     2100

cugcugcucu ccaccaacaa ccugaugcag auccugaccg acgagacgcu ccagaagaag     2160

augaccgagc ugaacgcgga caagcugaag accgccaaca ucaacgacgc caucgacaac     2220

gccuacacca gcccguccaa caagaaggcc cuccggcagg uccugcgcgu gguggacgac     2280

aucaagcggg cggccgacgg ccaggacccc agcuggcugu acgucgagac ggccgacggg     2340

ggcgggaccc ccggcaagcg cacccgggcc cgccagcacc agcuccagga gaucuacgcc     2400

aacgcggccc acgagcugau cgacaccgcc gugcggggcg agcuggagga caagaucucc     2460

gacaaggccg acuucaacga ccgccucgug cuguacuuca ugcagggcgg gcgggacauc     2520

uacacggggg cgccccugaa caucgaccag cucagcuccu acgacaucga ccacauccug     2580

ccgcagagcc ugaucaagga caacucccuc gacaaccgcg uccuggugca cgccaucauc     2640

aaccgggaga agaacgccac cuucgccagc accaucuacg cccagaagau gaacgcgacc     2700

uggcgccagu ggcacgaggc cggccugauc agcggccgga agcuccgcaa ccugcagaug     2760

cggcccgacc agaucgacaa guacgccucc ggguucgugg cccgccagcu ggucgagacg     2820

cggcagauca ucaagcucac cgagcagauc guggccgcgc aguaccccga caccaagauc     2880

aucgccguga aggccggccu gagccaccag cugcgcgagg agcucgaguu cccgaagaac     2940

cgggacguca accacuacca ccacgccuuc gacgccuucc uggcggcccg caucgggacc     3000

uaccugcuca agcgcuuccc caaccugcag gccuucuuca cguacggcaa guucaccaag     3060

gccgacguga agaagcugcg gggguucaac uucauccgcg acaucaccca cgccgaggac     3120

aagaucgugg cgaaggacac cggcgagguc gugugggaca agcagcggga cguggacgag     3180

cucgaccgca ucuacaacuu caagcggaug cugaucacgc acgagguccg cuucgagacc     3240

gccgaccugu ucaagcagac cguguacggc gcccgggacu ccaaggaggc cgggggcagc     3300

aagcagcuca uccccaagaa gaaggacauc cagcugaucu ucaugggggc gaucuccgag     3360

cgcacccggc ccauccugcc gcucuga                                         3387


<210>  1648
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1648
auggacgaga agauggacua ccgcaucggc cuggacaucg ggaucgccag cgugggcugg       60

gccguccucc agaacaacuc cgacgacgag cccgugcgga ucguggaccu ggggguccgc      120

aucuucgaca ccgcggagau cccgaagacc ggcgagagcc uggccgggcc ccggcgcgcc      180

gcccggacga cccgccggcg ccuccggcgc cggaagcacc gccuggaccg gaucaagugg      240

cuguucgaga accagggccu caucaacauc gacgacuucc ugaagcgcua caacauggcc      300

ggccugcccg acguguacca gcuccgguac gaggcgcugg accgcaagcu gaccgacgag      360

gagcucgccc aggugcugcu gcacaucgcc aagcaccggg gguuccgcuc cacccggaag      420

gccgagacgg cggccaagga gaacggcgcc guccucaagg ccaccgacga gaaccagaag      480

cgcaugcagg agaaggggua ccggaccgug ggcgagauga ucuaccugga cgaggccuuc      540

cgcaucgggu gcagcugguc cgagaagggc uacauccuga cgccccgcaa caaggcggag      600

aacuaccagc acaccaugcu ccgggccaug cugguggagg aggucaagga gaucuucagc      660

ucccagcgcc ggcugggcaa caagaaggcc accgaggagc ucgaggagaa guaccuggag      720

aucaugacca gccagcgcuc cuucgaccug gggccgggca ugcagcccga cgggaagccc      780

agccccuacg ccauggaggg cuucuccgac cgggugggca agugcaccuu ccucggggac      840

cagggcgagc ugcgcggggc caagggcacg uacaccgcgg aguacuucgu ggcccugcag      900

aagaucaacc acaccaagcu cgucaaccag gacggggaga cccggaacuu cacggaggag      960

gagcgccggg cccugacccu gcuccuguuc acccagaagg aggugaagua cgccgccgug     1020

cgcaagaagc ugggccuccc ggaggacauc cuguucuaca accugaacua caagaaggcg     1080

gccaccaagg aggagcagca gaaggagaac cagaacacgg agaaggccaa guucaucggc     1140

augcccuacu accacgacua caagaagugc cucgaggagc gggucaagua ccugaccgag     1200

aacgaggugc gcgaccuguu cgacgagauc gggaugaucc ugaccugcua caagaacgac     1260

gacagccgga ccgagcgccu cgccaagcug ggccucgugc ccaucgagau ggaggggcug     1320

cuggccuaca cgcccaccaa guuccagcac cucuccauga aggcgaugcg gaacaucauc     1380

ccguuccugg agaagggcau gaccuacgac aaggccugcg aggaggccgg guacgacuuc     1440

aaggccgaca gcaagggcac caagcagaag cugcucacgg gcgagaacgu caaccagacc     1500

aucaacgaga ucaccaaccc cguggugaag cgcuccguca gccagaccgu gaaggugauc     1560

aacgcgauca uccggacgua cgggagcccc caggccauca acaucgagcu ggcccgcgag     1620

auguccaaga ccuucgagga gcggcgcaag aucaagggcg acauggagaa gcgccagaag     1680

aacaacgagg acgucaagaa gcagauccag gagcugggga agcugucccc caccggccag     1740

gacauccuca aguaccggcu guggcaggag cagcagggga ucugcaugua cagcggcaag     1800

accaucccgc uggaggagcu guucaagccc ggcuacgaca ucgaccacau ccuccccuac     1860

agcaucacgu ucgacgacuc cuuccgcaac aaggugcugg ucaccagcca ggagaaccgg     1920

cagaagggga accgcacccc cuacgaguac augggcaacg acgagcagcg guggaacgag     1980

uucgagaccc gcgugaagac cacgauccgg gacuacaaga agcagcagaa gcuccugaag     2040

aagcacuucu ccgaggagga gcgcagcgag uucaaggagc ggaaccugac cgacaccaag     2100

uacaucacca cggugaucua caacaugauc cgccagaacc ucgagauggc cccgcugaac     2160

cggcccgaga agaagaagca gguccgcgcc gugaacgggg cgaucaccgc cuaccugcgg     2220

aagcgcuggg gccuccccca gaagaaccgg gagaccgaca cccaccacgc cauggacgcc     2280

guggucaucg ccugcugcac ggacggcaug auccagaaga ucucccgcua caccaaggug     2340

cgggagcgcu gcuacagcaa ggggaccgag uucguggacg cggagaccgg cgagaucuuc     2400

cggcccgagg acuacucccg cgccgagugg gacgagaucu ucggggucca caucccgaag     2460

cccugggaga cguuccgggc cgagcuggac gugcgcaugg gcgacgaccc caagggguuc     2520

cuggacaccc acagcgacgu ggcccucgag cuggacuacc ccgaguacau cuacgagaac     2580

cugcgcccga ucuucgucuc ccggaugccc aaccacaagg ugaccggcgc cgcgcacgcc     2640

gacaccaucc gcagcccccg gcacuucaag gacgagggca ucgugcucac gaagaccgcc     2700

cugaccgacc ugaagcucga caaggacggg gagaucgacg gcuacuacaa cccgcagucc     2760

gaccugcugc ucuacgaggc ccugaagaag cagcugcucc uguacgggaa cgacgcgaag     2820

aaggccuucg cccaggacuu ccacaagccc aaggccgacg gcaccgaggg gcccgucgug     2880

cgcaagguga agauccagaa gaagcagacg augggcgucu ucguggacag cggcaacggg     2940

aucgccgaga acggcgggau ggugcggauc gacgucuucc gcgugaacgg caaguacuac     3000

uucgugcccg ucuacaccgc ggacguggug aagaaggucc ugccgaaccg ggccuccacc     3060

gccaacaagc ccuacagcga guggcgcacc auggaggaca aggacuuccu cuucucccug     3120

uacagccggg accuggugca caucaagucc aagaagggga uccccaucaa gaacguggac     3180

ggcagccucg gcgaguccaa ggaggucuac gccuacuaca ucggggccga caucagcggc     3240

gcgaagaucg aggggaaggc ccacgacagc cgcuacacgu uccggggcau cggggugcag     3300

aagcuggagc ugaucgagaa gugccaggug gacauccucg gccacaucuc caccgucaag     3360

aacgagaagc gcaugggcuu cagcuga                                         3387


<210>  1649
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1649
augaacgaca agaaccagaa guaccgccug ggccucgacc uggggaccaa cagcaucggc       60

uggugcaucu acucccugga cgugaacaac aagcccgaga cccucaucga cauggggguc      120

cggaucuuca gcgacggccg cgacccgaag acgaaggagc cccuggccgu ggagcggcgc      180

accgcccggg ggcugcgccg gauccuccac cgccggaagc ugcgccggcg ccagauguuc      240

cggcugcucc aggagcaggg ccugaugccc aacgacgcgu ccgaggccgc cgcccucaag      300

agccucaacc ccuacgagcu gcgcgugaag gcccuggaca ccaagcucga gccguacgag      360

cugggccggg cgcuguucaa ccucuccguc cgccgggggu ucaagagcaa ccgcaaggag      420

gccgugcagg agaaggagaa ggacucccag aacgacgcca agagccagaa cgagaagugc      480

gagcaccugg ccaaggcgau caaggacucc ggcugccgga cccuggggga guuccucugg      540

aagcacaaga acacggagac ccagcagacc gagaacgacg cccgcaacau cggcaugcgg      600

uucgugcccg ggcgcagcaa cuacuacccc acccgccgga uguacgucga ggaguucgac      660

gccauccgca aggcccagga gcccuacuac uccggcguga acugggacgc ccugcacgag      720

aagcuguucu accagcggcc gcuccgcgcg caggagcggg gcaagugccg cuacaugccc      780

gacaaggagc ggacguucaa ggccaugccc ugcagccaga agauccgcau ccugcaggag      840

guguacaaca uggacuacau cgacgaguac gggaagacca ucccccugga ggaggcccag      900

accgacuccc ucaucagccu gcuggacaag cgggagaagc ucuccuucaa ggccaugaag      960

aaggagcugg gcaucgacga gagcugcacc uucaaccugg agcgcggggg ccgggaguac     1020

cuccagggca acgccgcggc cgucaagcug cgcaacaaga agcgguucgg ggagcugugg     1080

gacucccuca gccuggccaa gcaggacgcc aucguggaga agcugaucac ggccgacgag     1140

gacgcggaga ucaucuccau gcucgcccag uacaaccuga ccgacgagca gaagaagaac     1200

aucgccguga ccguccugcc gagcggcacc gccaugcucu gcaaggaggu gacggagaug     1260

cuggugcaga agauggagca gaacaagauc ccccugaccg ucgccgugca gaugcucggg     1320

uacaccuacg cggaccagag cgugcgcgag gaggacaccc ugcccuacua cggcgccauc     1380

cugacggggu ccaccauggg cgcccacccc gaggccgacg agagcgagcc ggagcucaag     1440

uacggcaaga ucuccaaccc caccguccac guggcgcuga accagacccg gacggugguc     1500

aacgcccuga ucaaggccua cgggaagccc cagcagaucg ugguggagcu gagccgcgac     1560

cucaaggccu cccgggaggc caaggcgcgc auccagaaga ccaucaccau gaaccagaag     1620

cggaacgagc gcgacaacaa gaacaucacc gacaucacga agauccccua cccgaaccgc     1680

aacgaccggc ugaaguaccg ccugugggag gagcucggca gcggguccaa cagcuucucc     1740

cggaagugcc uguacugcgg caagcccauc agcggguccg agaucuucac cgagaacauc     1800

gagaucgagc acauccugcc cuucggccgc acccucuucg acggcgagac caacaagacg     1860

gucgcccaca ccgacugcaa ccgggacaag ggggaccgca gccccuucga ggccuucggc     1920

uccagcccga acggguacaa cuggcacgag aucugcgccc gggccaacuc ccugaagaac     1980

cccgcgaagc gcacccuguu cgccgagaac gccauggaga aguucgagaa ggacaacagc     2040

uucauccagc ggcagcucac cgacaacgcc uaccuguccc gcagcgcccu gcgguaccuc     2100

cgcugcaucu gcaaggacgu gugguccauc aacggcggga ugacgaagcu gcugcgggac     2160

aaguggaaca ucgacagcau ccucaagcgc aagaucgacg accccgagau cgcgcacuuc     2220

gagcugaaga acgaccagau cggccaguac aagaagaacc gguacgacca ccgccaccac     2280

gcccuggacg ccagcgucau cgcccucauc gaccgggcga uggugcagga gaucucccag     2340

cugaacaaga uccgccugaa gaaccggauc gaggcgcccc agaugccggu gcuccgcagc     2400

gagcugaucg agaaggucaa gcacaucacc guguccuuca agcccgacca cggccggcag     2460

gggaagcuga gcaaggagac ccuccugggc aagaucaaga agaucgagaa gguggacauc     2520

gacuccaucc agaccgacga caucgccagc aucaaggacg acgccauccg cacccgcuuc     2580

gagacgaagc ggcgcgagcu gggggacauc accaaggcca aggccgcccu caagaccgag     2640

uuccccaagc uggacgucuu caagaccuac uacguguccc ggacgccgau cgugagccug     2700

accgagaaga acaucaacuc caucgucgac gagaagauca aggagaagcu caagaccuuc     2760

aucaaggagc acgccgaccu gagcuucgcc gagcagcugc agaaguucuc cgagaccgag     2820

uggaucggca agcccagcgc cgcgggcaag cccgggaaga aguaccgcau cgagaaggug     2880

cggugcauca acuggggcca gacgcccauc accaucaccu ccagcgccgu gccgcgcuac     2940

cucugccccg aggacuaccu ggccgccguc guguggcaga uccccuccaa gaagaaggac     3000

accgagcccg aguacaaggc cacguuccug cggcgcgacg aguucgacgu gaacaacaag     3060

ccgaagaagc ucgagaagcc ccacgacgcg gcccaguaca ucugcaugcu guacaaggac     3120

gacuaccugg aguucaccga gagcggggcc acccaccucu gccggaucgc cggccugccc     3180

gcgucccaga acaacaagau cgacauccgc cccaucuacg ccgucagcga cugcaaggac     3240

uggaucaucg ccaccaacga gaacaugcug gagccgugcu ggaagcccca gaaggaggag     3300

aagcggauca gcgugaacgu gcuguucggg aagcacaagg cccgcuccgu cacggugaac     3360

cccaucggcc ggguguuccg caaguga                                         3387


<210>  1650
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1650
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacg ucgagcggcg caccaaccgg cagggccgcc ggcucacgcg ccggaagaag      180

caccgccggg ugcgccugaa ccaccuguuc gaggaguccg gccucaucac cgacuucacc      240

aaggugagca ucaaccugaa cccguaccag cugcggguca aggggcucau cgacgagcug      300

uccaacgagg agcuguucau cgcccucaag aacaugguga agcaccgcgg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgucaag      420

gagaacagca agcagcugga gaccaagacg cccgggcaga uccagcucga gcgguaccag      480

aaguacggcc agcugcgcgg cgacuucacc guggaggagg acgggaagaa gcaccggcug      540

aucaacgugu uccccaccuc cgccuaccgc agcgaggcgc uccggauccu gcagacccag      600

aaggaguuca acccccagau cacggacgag uucaucaacc gcuaccugga gauccucacc      660

ggcaagcgga aguacuacca cgggccgggc aacgagaagu cccgcaccga cuacggccgg      720

uacaccacga agaaggacag cgagaacgag uacaucaccc uggacaacau cuucgggauc      780

cugaucggca agugcaccuu cuaccccgag gaguaccgcg ccgccaaggc cuccuacacc      840

gcgcaggagu ucaaccuccu gaacgaccug aacaaccuca cgguccccac cgagaccaag      900

aagcugagcg aggagcagaa gaaccagauc aucaacuacg ugaagaacga gaaggccaug      960

gggcccgcca agcuguucaa guacaucgcc aagcuccugu ccugcgacgu ggccgacauc     1020

aagggcuacc gcaucgacaa gagcgacaag gcggagaucc acaccuucga ggccuaccgg     1080

aagaugaaga cgcuggagac caucgacuuc gagaagaugu cccgcgacca gcucgacaag     1140

cuggccuacg uccugacccu caacaccgag cgggagggga uccaggaggc ccuggaccac     1200

gaguucgccg acggcaacuu cagccaggag cagaucgacg agcuggugca guuccgcaag     1260

gcgaacagcu ccaucuucgg caaggggugg cacaacuuca gcgugaagcu caugauggag     1320

cugaucccgg agcuguacgc cacguccgag gagcagauga ccauccucac ccggcugggc     1380

aagcagaaga ccacgagcuc cagcaacaag accaaguaca ucgacgagaa gcugcucacc     1440

gaggagaucu acaaccccgu cguggccaag uccgugcgcc aggccaucaa gaucgucaac     1500

gccgcgauca aggaguacgg ggacuucgac aacaucguga ucgagauggc ccgggagacc     1560

aacgaggacg acgagaagaa ggccauccag aagauccaga aggccaacaa ggacgagaag     1620

gacgccgcga ugcugaaggc cgccaaccag uacaacggcc gcgccgagcu gccccacagc     1680

guguuccacg ggcacaagca gcucgcgacg aagauccggc uguggcacca gcagggcgag     1740

cgcugccugu acaccggcaa gaccaucucc auccacgacc ugaucaacaa ccccaaccag     1800

uucgagaucg accacauccu cccgcugagc aucaccuucg acgacucccu ggccaacaag     1860

guccucgugu acgccacggc caaccaggag aaggggcagc ggacccccua ccaggcccug     1920

gacagcaugg acgacgcgug guccuuccgc gagcugaagg ccuucguccg ggagagcaag     1980

ucccucagca acaagaagaa ggaguaccug cugaccgagg aggacaucuc ccgcuucgac     2040

gugcggaaga aguucaucga gcgcaaccuc guggacaccc gguacgccag ccgcgucgug     2100

cugaacgccc ugcaggagca cuuccgggcc cacaagaccg acacgaaggu guccgucgug     2160

cgcggccagu ucaccagcca gcuccgccgg cacuggggga ucgagaagac ccgcgacacc     2220

uaccaccacc acgcggugga cgcccugauc aucgccgcca gcucccagcu gaaccucugg     2280

aagaagcaga agaacacgcu ggucaacuac agcgagaacc agcugcucga caucgagacc     2340

ggcgagcuga ucuccgacga cgaguacaag gagagcgugu ucaaggcccc cuaccagcac     2400

uucguggaca cccugaaguc caaggaguuc gaggacagca uccucuucuc cuaccagguc     2460

gacagcaagu ucaaccggaa gaucuccgac gcgaccaucu acgccacgcg ccaggccaag     2520

guggggaagg acaagaagga cgagaccuac gugcugggca agaucaagga caucuacagc     2580

cagaccggcu acgacgccuu caucaagauc uaccagaagg acaaguccaa guuccugaug     2640

uaccggcacg acccccagac cuucgagaag gucaucgagc cgauccucga gaacuacccc     2700

aacaaggaga ugaacgagaa ggggaaggag gugcccugca accccuuccu gaaguacaag     2760

gaggagcacg gcgacuacau ccgcaaguac agcaagaagg ggaacggccc ggagaucaag     2820

ucccugaagu acuacgacag caagcucggc aaccacaucg acaucacgcc caaggacucc     2880

aacaacaagg ugguccugca gagcgugucc cccuggcggg cggacgugua cuucaacaag     2940

accaccggga aguacgagau ccugggccuc aaguacgccg accugcaguu cgagaagggg     3000

accggcacgu acaagaucag ccaggagaag uacaacguca ucaagaagaa ggagggggug     3060

gacagcgacu ccgaguucaa guucacccug uacaagaacg accuccugcu gaucaaggac     3120

accgagacca aggagcagca gcuguuccgc uuccucagcc ggacgaagcc caacgugaag     3180

cacuacgucg agcugaagcc guacgacaag cagaaguucg agggcaacga gucccucauc     3240

aacgugcugg gcgccguggc caagggcggg cagugccaga aggggaucaa caagcccaac     3300

aucagcaucu acaagguccg caccgacgug cugggcaacc agcacaucau caagaacgag     3360

ggggacaagc ccaagcucga cuucuga                                         3387


<210>  1651
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1651
auggacgaga agauggacua ccgcaucggc cuggacaucg ggaucgccag cgugggcugg       60

gccguccucc agaacaacuc cgacgacgag cccgugcgga ucguggaccu ggggguccgc      120

aucuucgaca ccgcggagau cccgaagacc ggcgagagcc uggccgggcc ccggcgcgcc      180

gcccggacga cccgccggcg ccuccggcgc cggaagcacc gccuggaccg gaucaagugg      240

cuguucgaga accagggccu caucaacauc gacgacuucc ugaagcgcua caacauggcc      300

ggccugcccg acguguacca gcuccgguac gaggcgcugg accgcaagcu gaccgacgag      360

gagcucgccc aggugcugcu gcacaucgcc aagcaccggg gguuccgcuc cacccggaag      420

gccgagacgg cggccaagga gaacggcgcc guccucaagg ccaccgacga gaaccagaag      480

cgcaugcagg agaaggggua ccggaccgug ggcgagauga ucuaccugga cgaggccuuc      540

cgcaccgggu gcagcugguc cgagaagggc uacauccuga cgccccgcaa caaggcggag      600

aacuaccagc acaccaugcu ccgggccaug cugguggagg aggucaagga gaucuucagc      660

ucccagcgcc ggcugggcaa cgagaaggcc accgaggagc ucgaggagaa guaccuggag      720

aucaugacca gccagcgcuc cuucgaccug gggccgggca ugcagcccga cgggaagccc      780

agccccuacg ccauggaggg cuucuccgac cgggugggca agugcacguu ccucggggac      840

cagggcgagc ugcgcggggc caagggcacc uacaccgcgg aguacuucgu ggcccugcag      900

aagaucaacc acaccaagcu cgucaaccag gacggggaga cgcggaacuu caccgaggag      960

gagcgccggg cccugacccu gcuccuguuc acccagaagg aggugaagua cgccgccgug     1020

cgcaagaagc ugggccuccc ggaggacauc cuguucuaca accugaacua caagaaggcg     1080

gccacgaagg aggagcagca gaaggagaac cagaacaccg agaaggccaa guucaucggc     1140

augcccuacu accacgacua caagaagugc cucgaggagc gggucaagua ccugaccgag     1200

aacgaggugc gcgaccuguu cgacgagauc gggaccaucc ucacgugcua caagaacgac     1260

gacagccgga ccgagcgccu ggccaagcug ggccucgugc ccaucgagau ggaggggcug     1320

cuggccuaca cccccaccaa guuccagcac cucuccauga aggcgaugcg gaacaucauc     1380

ccguuccugg agaagggcau gacguacgac aaggccugcg aggaggccgg guacgacuuc     1440

aaggccgaca gcaagggcau caagcagaag cugcucaccg gcgagaaggu caaccagacc     1500

aucaacgaga ucaccaaccc cguggugaag cgcuccguca gccagacggu gaaggugauc     1560

aacgcgauca uccggaccua cgggagcccc caggccauca acaucgagcu ggcccgcgag     1620

auguccaaga ccuucgagga gcggcgcaag aucaagggcg acauggagaa gcgccagaag     1680

aacaacgagg acgucaagaa gcagauccag gagcugggga agcugucccc caccggccag     1740

gacauccuca aguaccggcu guggcaggag cagcagggga ucugcaugua cagcggcaag     1800

acgaucccgc uggaggagcu guucaagccc ggcuacgaca ucgaccacau ccuccccuac     1860

agcaucaccu ucgacgacuc cuuccgcaac aaggugcugg ucaccagcca ggagaaccgg     1920

cagaagggga accgcacccc cuacgaguac augggcaacg acgagcagcg guggaacgag     1980

uucgagaccc gcgugaagac gaccauccgg gacuacaaga agcagcagaa gcuccugaag     2040

aagcacuucu ccgaggagga gcgcagcgag uucaaggagc ggaaccugac cgacaccaag     2100

uacaucacga ccgugaucua caacaugauc cgccagaacc ucgagauggc cccgcugaac     2160

cggcccgaga agaagaagca gguccgcgcc gugaacgggg cgaucaccgc cuaccugcgg     2220

aagcgcuggg gccuccccca gaagaaccgg gagaccgaca cgcaccacgc cauggacgcc     2280

guggucaucg ccugcugcac cgacggcaug auccagaaga ucucccgcua caccaaggug     2340

cgggagcgcu gcuacagcaa ggggaccgag uucguggacg cggagacggg cgagaucuuc     2400

cggcccgagg acuacucccg cgccgagugg gacgagaucu ucggggucca caucccgaag     2460

cccugggaga ccuuccgggc cgagcuggac gugcgcaugg gcgacgaccc caagggguuc     2520

cuggacaccc acagcgacgu ggcccucgag cuggacuacc ccgaguacau cuacgagaac     2580

cugcgcccga ucuucgucuc ccggaugccc aaccacaagg ugaccggcgc cgcgcacgcc     2640

gacacgaucc gcagcccccg gcacuucaag gacgagggca ucgugcucac caagaccgcc     2700

cugaccgacc ugaagcucga caaggacggg gagaucgacg gcuacuacaa cccgcagucc     2760

gaccugcugc ucuacgaggc ccugaagaag cagcugcucc uguacgggaa cgacgcgaag     2820

aaggccuucg cccaggacuu ccacaagccc aaggccgacg gcacggaggg gcccgucgug     2880

cgcaagguga agauccagaa gaagcagacc augggcgucu ucguggacag cggcaacggg     2940

aucgccgaga acggcgggau ggugcggauc gacgucuucc acgugaacgg caaguacuac     3000

uucgugcccg ucuacaccgc ggacguggug aagaaggucc ugccgaaccg cgccgccacc     3060

gcccacaagc ccuacgggga guggaaggug auggaggaca aggacuuccu cuucucccug     3120

uacagccggg accugaucca caucaagucc aagaaggaca uccccaucaa gauggugaac     3180

ggcggcaugg aggggaucaa ggagacguac gccuacuaca ucggcgcgga caucagcgcc     3240

gccaacaucc aggggaucgc ccacgacucc cgcuacaagu uccggggccu cgggauccag     3300

agccuggacg uccuggagaa gugccagauc gacgugcucg gccacguguc cgucgugcgc     3360

agcgagaagc ggaugggcuu cagcuga                                         3387


<210>  1652
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1652
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cguccacaag aacgcccgca ucuuccccgc cgcgcaggcc      120

gagaacaacg uggagcggcg caccaaccgg cagggccgcc ggcucacgcg ccggaagaag      180

caccgccggg ugcgccugaa ccacaucuuc gaggagagcg gccugaucac cgacuucacc      240

aaggucucca ucaaccucaa cccguaccag cugcggguga aggggcugac cgacgagcuc      300

agcaacgagg agcuguucau cgcccugaag aacaugguga agcaccgcgg caucuccuac      360

cucgacgacg ccagcgacga cgggaacucc agcgucggcg acuacgccca gaucgugaag      420

gagaacucca agcagcugga gacgaagacc cccgggcaga uccagcugga gcgguaccag      480

aaguacggcc agcuccgcgg cgacuucacc aucgaggagg acgggaagaa gcaccggcug      540

aucaacgugu uccccaccag cgcguaccgc gccgaggccc ugcggauccu ccagacgcag      600

caggaguuca acccccagau caccgacgag uucaucaacu ccuaccugca gauccugacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggaccga cuacggccgc      720

uacacgacca agaaggacag cgaggacgag uacaucaccc ucgacaacau cuucgggauc      780

cugaucggca agugcaccuu cuaccccgag gaguaccggg ccgcgaaggc cagcuacacg      840

gcccaggagu ucaaccugcu caacgaccug aacaaccuga ccguccccac cgagaccaag      900

aagcucuccg aggagcagaa gaaccagauc aucacguacg ugaagaacga gaaggccaug      960

gggcccgcca agcuguucaa guacaucgcg aagcugcucg ccugcgacgu ggccgacauc     1020

aagggcuacc gcaucgacaa gagcgacaag ccggagaucc acaccuucga ggccuaccgc     1080

aagaugaaga cccuggagac caucgacguc gagcagaugg agcgggagaa gcuggacaag     1140

cucgccuacg ugcugacgcu gaacaccgag cgcgagggga uccaggaggc gaucgagcac     1200

gaguucuccg acggcaccuu cagccaggag caggucgacg agcucgugca guuccggaag     1260

uccaacagca gcaucuucgg caaggggugg cacaacuucu ccgugaagcu gaugauggag     1320

cugauccccg agcucuacgc caccagcgag gagcagauga cgauccugac ccgccugggc     1380

aagcagaagc ggaccuccag cuccaacaag accaaguaca ucgacgagaa gcgccucacg     1440

gaggagaucu acaaccccgu ggucgccaag agcgugcggc aggccaucaa gaucgugaac     1500

gccgcgauca aggaguacgg ggacuucgac aacaucguca ucgagauggc ccgcgagaug     1560

aacgaggacg acgagaagaa ggccaucaag aagauccagg aggccaacaa guccgagaag     1620

gacgccgcga ugcggaaggc cgccaaccag uacaacggca aggccgagcu gccccacagc     1680

guguuccacg ggcacaagca gcuggcgacc aagauccgcc ucuggcacca gcagggcgag     1740

cggugccugu acaccggcaa gaccaucucc auccacgacc ugaucaacaa cccgaaccag     1800

uucgagaucg accacauccu cccccugagc aucacguucg acgacucccu ggccaacaag     1860

guccuggugu acgccaccgc caaccaggag aaggggcagc gcacccccua ccaggcccuc     1920

gacagcaugg acgacgcgug guccuuccgg gagcugaagg ccuucgugcg cgagagcaag     1980

acccugucca acaagaagaa ggaguaccuc cugaccgagg aggacaucag caaguucgac     2040

guccggaaga aguucaucga gcgcaaccug guggacacgc gguacgccuc ccgcgugguc     2100

cucaacgccc ugcaggagca cuuccgggcc cgcaagaucg acaccaaggu gagcgugguc     2160

cgcggccagu ucaccuccca gcugcggcgc cacuggggga ucgacaagac ccgggacacg     2220

uaccaccacc acgcggugga cgcccucauc aucgccgcca gcagccagcu gaaccugugg     2280

aagaagcaga agaacacccu cguguccuac agcgaggacc agcugcugga caucgagacc     2340

ggcgagcuca ucuccgacga cgaguacaag gagagcgucu ucaaggcccc cuaccagcac     2400

uucguggaca cccugaaguc caaggaguuc gaggacagca uccuguucuc cuaccaggug     2460

gacagcaagu ucaaccgcaa gaucuccgac gcgacgaucu acgccacccg gcaggccaag     2520

gucgggaagg acaagaagga cgagaccuac gugcucggca agaucaagga caucuacagc     2580

caggccggcu acgacgcguu caucaagauc uaccagaagg acaaguccaa guuccugaug     2640

uaccgccacg acccgcagac cuucgagaag gugaucgagc ccauccugga gaacuacccc     2700

aacaaggaga ugaacgagaa ggggaaggag guccccugca acccguuccu caaguacaag     2760

gaggagcacg gcgacuacau ccggaaguac agcaagaagg ggaacggccc cgagaucaag     2820

ucccugaagu acuacgacag caagcugggc aaccacaucg acaucacgcc caagaacucc     2880

aacaacaagg uggugcucca gagcgucucc cccuggcgcg ccgacgugua cuucaacaag     2940

accaccggga aguacgagau ccugggccug aaguacgccg accuccaguu cgagaagggg     3000

accggcacgu acaagaucag ccaggagaag uacaacgaca ucaagaagaa ggagggggug     3060

gacagcgacu ccgaguucaa guucacccug uacaagaacg accugcuccu gaucaaggac     3120

accgagacca aggagcagca gcuguuccgg uuccucagcc gcuccaugcc gaacaagaag     3180

cacuacgucg agcugaagcc cuacagcaag gacaaguucg agaagaacga gacgcugauc     3240

gagauccucg gccccaccga caaguccggc cggugcauca aggggcuggc caagagcaac     3300

cuguccaucu ucaaggugcg caccgacauc cucggcaacc agcacaucau caagaacgag     3360

ggggacaagc ccaagcugga cuucuga                                         3387


<210>  1653
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1653
augaccaaga uccugggccu cgaccugggg accaacagca ucggcugggc cguggucgac       60

gagaagaagg agaagauccu ggggaugggc ucccgcaucu uccccgaggg gguggugaac      120

aucggcgagg gcgaggggcg ggagacgagc aagaacgccu cccgcaccga cgcgcggggc      180

acccgccggc aguucuuccg ccggcgccuc cggaagcgcu accugcugcg ggagcucgcc      240

aagaaggggc ugugcccgau caacuacacc cuggucaagg aguggaacga ggcccagauc      300

uucaagaaca agcagcucca ggagugguuc aagcugaacc ccuacgagcu gcgcagcaag      360

gccauccagg agaagaucuc ccucgaggag cugggccgga ucuucuacca cuucauccag      420

cgccgggggu uccagagcaa cucccgcagc gccggcgugg acaacaucga gaaguccgug      480

aucuucaagg gcgacacgaa gaccgggaag aucggcauca gcgagacccu ggaguccauc      540

aagaacacca agacgcucgg gagcuaccug cacaccaucu accccaagca gaacaagccc      600

uacuccggcc acaaggagcg gauccgcaac cgguacacca cccgccagau guacaucuac      660

gaguucgaga cgaucuggga guaccagaag caguucuaca gcgagcuguc caacgagcuc      720

aagaccgucc ugggcgggcg gaagaaggac ggcuacaagg aggacgggau ccuguuccac      780

cagcgcccgc uccgcagcca gaagcaccug gugggcuacu gcuccuucga gcccaagaag      840

accaagugcc agaucaacac cauccccuuc gagcuguucc ggaucuacca gugggugaac      900

acggucgacu gcaacgagaa ggugaccagc gaggaccgcg ggaagauggu ggagcuccug      960

cuguccaagg agaaggucag cuucaaggag auccggaagg ugaucaacaa gagcgacggc     1020

uacuaccagu ucaacuacaa gaacgacgac aagaucgugg gcuccuacac caucagcaac     1080

cucuccaaca agaaguucuu cgggaagaag ugguucgacu ucagcgacaa ggagcaggag     1140

gacaucuggc acguccuguc cuucuucgac gaccgcgaca agcugaagca guacgcgauc     1200

aaguacuggg gcuucaacga ggagaaggcc gugaagauca gcaaguucaa ccucaaggag     1260

ggguacucca accugagccg gaaggccauc cacaacaucc ugcccuuccu caagcugggc     1320

uucauguacg acguggccgu cgggcugggc ggcgugaaga acgcgcucgc cgacaagugg     1380

aucgagaacg agcaguucau ccuggacaac gugccguccg ucgugcgcag caacuucaag     1440

ggcggguaca ucgagccccu gaagcagaug cucaaguacg aguucgacgu guccgacaag     1500

cagcugcuga agcucuacca ccacagcucc gccaucgaga ccacggagcu gcugaagaag     1560

cuccccauca acagcgaggc cgacaaggag auccagucca ucaagaaccc cgucgugauc     1620

accgcccugu ucgagauccg gaagcugguc aacgagauca ucgacaccua cgggaaguuc     1680

gacgagauca agguggagcu cgcgcgcgac cugaaggcca gcaaguccaa gcgguacgac     1740

acccgccggg agcagcugcg ccuggagaag gagaacgacc ggaucaagau cgagcucgag     1800

aaccugggcc agcgcaucaa ccacaucacg auccugaagu acaagcucug ggaggagugc     1860

gacaagcggu gcccguucac cgggaagaac aucgagguga acgagcuguu cagcggcgag     1920

guccagaucg agcacaucuu ccccuggucc cgcagccuga acgacagcuu caugaacaag     1980

acccucugcu ucgccgacga gaaccgggcc aagggcaaca agacccccua cgaguucuac     2040

uccgagcagg gggagcagaa gugggaggag aucaagaucc aggcccugag cugcuucaag     2100

aacaagccca acuacccgaa cgcguaccag aaguucaagc aguucgugaa gaagaagcac     2160

gacgaggacu ucaucucccg ccagcugaac gacacgcggu acaucagcaa ggaggccaag     2220

aacuaccucu ccaagaucug caacaacgug caggucgccc ccggccagau gaccgccacc     2280

cugcgccaca agugggggcu gaacaccauc cucaacaagg acgacgacac gaagacccgc     2340

gacgaccacc ggcaccacgc caucgacgcg cuggugaugg ccugcagcac ccgcucccac     2400

cuccaggagc ugagcaagcg gaaccgcuac cagaaggacu acgaguucaa ggacuucccc     2460

augcccuggc aguucuuccg gcaggacgcc gagaaguccg uggagcagau ccuggucagc     2520

cacaagaagc ugaacaccau ccucacggug cgcacccaca agaccaagaa gaacggcgug     2580

gucuacaaga acaucggcgu ggccgcccgg gggcagcugc acaaggagac cguguucggc     2640

aagcgcaccg cgccggacgg gaucgaggcc uaccacgucc ggaagcccau cgagucccug     2700

agcacgcaca agcacaucaa caagguggug gacgagacca uccgccuccu gauccugaag     2760

caggucaaca agcucggcgg guucgugaag ggcaagaucc ccgagggcac cuucuacaac     2820

accgacgaga acgggaacaa gcagccccag aucuuccugc cgaacaagaa cggcaacccc     2880

gugcccauca agaagauccg gcugcgcgag aacaucgggg gcgccgagca gcucaagucc     2940

acggucaacc agcacgugaa cccccggagc aaccaccacg ugcugaucua caaggacuuc     3000

gacgacaacc ugaaggagaa ggucgugacc uucuggaccg ccguggagcg caagaagcag     3060

aagcaggcgg ucuuccagcu cccggagaac gggaaggaga ucgugaccac gcugcagauc     3120

aacgacaugu uccugcucgg ccugaaggac gacgagauca acugggagca ccccaaguuc     3180

acccugcuca aggagcaccu guaccgggug cagaaguuca ccuccggcga cuacuacuuc     3240

cgcaagcaca ucgagagcaa ccugaacggg gagcucggca aggccuacca guacaucaag     3300

ggguucggcg aggggaagac cggcuggaag acguucaacc ccaucaagau caaggucucc     3360

gugagcggca agaucgagaa gguguga                                         3387


<210>  1654
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1654
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggc cggcauccug       60

aagaaggaca ccggggagau cauccacgcg aacucccgcc ucuuccccgc cgccaccgcc      120

gacaacaacg ugcugcggcg cagcucccgg caggcccgcc ggagcacgcg ccggaagcgc      180

caccgggcgg ugcgccugca cgaccucuuc gaggacuacg gccugcugac cgacuucucc      240

aaggucagca ucaaccucaa cccguacaag cugcgggugg agggcaugga cauccagcug      300

accaccgagg agcuguucau cgcccucaag aacaucauca agcgccgggg gaucuccuac      360

cuggacgacg ccagcgagga cggcgugacg gucuccagcg acuacgggaa ggccguggag      420

gagaaccgca agcuccuguc cgagcagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc aggugcgcgg ggacuucacc gucgaggaga acggcgagaa gcggcgccuc      540

aucaacgugu ucagcaccuc cacguaccgg aaggaggcgg agcgcauccu gcggaagcag      600

caggaguuca acaacaagau caccgacgac uucaucgacg acuaccugac cauccucacc      660

gggaagcgca aguacuacca cggccccggg aacaagaagu cccgcacgga cuacggccgg      720

uacaccacca agaaggacag cgagggcaag uacaucaccc uggacaacau cuucggggug      780

cugaucggca agugcacguu cuaccccgac gaguaccgcg ccagcaaggc cuccuacacc      840

gcccaggagu ucaaccuccu gaacgaccug aacaaccuca ccgucccgac cgagacgaag      900

aagcugagcg agaagcagaa gaagaccauc guggaguacg ccaagaccgc gaagacccug      960

ggggccucca cgcuccugaa guacaucgcc aagauggugg acgccagcgu cgaccagauc     1020

cacggcuacc ggguggacgu gaacaagaag cccgagaugc acaccuucga cgucuaccgc     1080

aagaugcaga gccuggagac cuucaacgug aacgagaugc cccgggaggu gcucgacgag     1140

cuggcccaca uccugacccu caacacggag cgcgagggga ucgaggaggc gaucaacacc     1200

aagcugaagg gcaccuucaa ccaggaccag guccuggagc ucgugcaguu ccggaagaac     1260

aacuccagcc uguucuccaa gggcuggcac aacuucagcc ugaagcucau gauggagcug     1320

auccccgagc uguacgccac cuccgaggag cagaugacga uccucacccg ccuggggaag     1380

cagaagucca aggagaucag caagcggacc aaguacaucg acgagaagga gaucaccgag     1440

gagaucuaca acccgguggu cgccaagagc gugcgccagg ccaucaagau caucaacgag     1500

gccacgaagc aguacggcau cuucgaccac aucgucaucg agauggcgcg gcugaccaac     1560

gaggaggacg ccaagaagga cuacaucaag cgccagaagg ccaaccucga cgagaagaac     1620

gccgccaugg agaaggcggc cuuccaguac aacgggaaga aggagcugcc cgacuccauc     1680

uuccacggcc acaaggagcu ggccaccaag auccggcugu ggcaccagca gggggagaag     1740

ugccucuaca ccggcaagaa cauccccauc agcgaccuga uccagaacca guacaaguac     1800

gagaucgacc acauccugcc ccucucccug agcuucgacg acucccugag caacaaggug     1860

cucgugcugg ccaccgcgaa ccaggagaag ggccagcgca cgccguucca ggcccuggac     1920

cggauggacg acgccugguc cuaccgcgag uucaagagcu acgucaagga cuccaagcuc     1980

cuggggaaca agaagaagga cuaccugcuc accgaggagg acaucagcaa gaucgaggug     2040

aagcagaagu ucaucgagcg gaaccuggug gacacccgcu acuccagccg ggucgugcug     2100

aacgcccucc aggacuucua caagaaccac cacuucgaca ccacgaucag cgugguccgc     2160

ggccaguuca ccucccagcu gcgccggaag ugggggcugg agaagucccg cgagaccuac     2220

caccaccacg ccguggacgc gcucaucauc gccgccagca gccagcugcg gcuguggaag     2280

aagcagaaca acccccucau cuucuacaag gagggccagu ucguggacuc cgagaccggg     2340

gagauccuga gccugaacga cgacgaguac aaggagcucg ucuucaaggc ccccuacgac     2400

cacuucgugg acacgcugcg cuccaagacc uucgaggaca gcauccuguu cuccuaccag     2460

guggacagca aguacaaccg gaagaucucc gacgccacca ucuacgcgac ccgcaaggcc     2520

aagcucgaca aggacaagag caaggagacg uacguccugu ccaagaucaa ggacaucuac     2580

agccaggccg gcuacgacgc cuucaucaag aucuacaaca aggacaaguc caaguuccug     2640

auguaccaca aggacagcca gaccuucgag aaggugaucg aggagauccu ccggaccuac     2700

cccuccaagg aguucaacga caagaacaag gaggugccgu gcaaccccuu cgagaaguac     2760

cgccaggaga acggccccau ccggaaguac agcaagaagg ggaacggccc cgagaucaag     2820

agccugaagu acuacgacaa gaacaucccg gaguccuuca ucaacaucac ccccagcaac     2880

uccaagaaca aggucauccu gcgccagcuc aaccccuggc ggacggacgu guacuucaac     2940

caccagaccg agaaguacga gcugcugggg cucaaguacg ccgaccugca guucgagaag     3000

aagaccggca ccuacagcau cacgaucgac aaguacaacg agaucaagga gaaggagggc     3060

gugggggagc aguccgaguu caaguucacc cuguacaaga acgaccuccu gcugaucaag     3120

gacaccaaga acaacgagaa gcagaucuuc cgcuuccuca gccggaccau gcccaacguc     3180

aagcacuacg uggagcugaa gccguacaac aaggcgaagu ucgacggcaa ccagccccug     3240

aucacgaucu ucgggaacgu ggccaagggc gggcagugcc ucaagggccu gaacaagccc     3300

aacaucucca ucuacaagau caagaccgac guccugggca acaaguacuu caucaagaag     3360

gagggggaca agcccaagcu caaguucuga                                      3390


<210>  1655
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1655
auggccgacc gcaucagccu gggccucgac aucggggugg ccuccgucgg cuucagcgug       60

cuggacaucg acaaggggaa ggugaucgag cugggcuccc gccucuucaa cgcgaccguc      120

gccgagggga accaggaccg gcggagcaug cgcggcgccc ggcgccugcu gaaccggaac      180

aagcagcgcc ggcaggacac cggcaagcug uuccagaagu ucgggcuccu ggacaacuuc      240

aacaaggaca acuucuacga cacguucaac cagaacugga accccuacga gcuccgcgug      300

aagggccuga ccgagcaccu gaccaagaag gagcucgccg agucccugua ccagaucgug      360

aagcaccggg ggaucagcua cgcccugaag gacgcggacg ccgacgaggg cgggaccgac      420

uacuccgcca gccucaagau caacucccag gagcuggccg agaagacgcc ggcgcagauc      480

cagcugcagc gccucaacga cuacggcaag guccggggcc agguggugau cggggacgac      540

cccgacaacc agaagguccu gcugaacgug uuccccacca gcgccuacga gaaggaggcc      600

aagcagauca ucgccaccca gaagcaguuc uaccccgaga uccucaccga cgaguucacg      660

aaggaguacu gcaagauccu gacccgcaag cgggacuacu ucgugggccc ggggaacgag      720

aagucccgca ccgacuacgg caucuacaag accgacggcc ggacgcugga caaccucuuc      780

gaggagcuga ucgggcacga caagaucuac cccgacgagc ugcgcgccag cgcggccucc      840

uacaccgccc agcuguucaa cguccucaac gaccugaaca accuccggau ccugagcuac      900

gaggacggca agcugaccaa ggaggacaag gagaagauca ucgccgagau caaguccaac      960

accacgacca ucaacaugcu caacgugauc aagaaggugg ccgggugcag caaggaggac     1020

aucaagggcu uccgcgucaa cgagaaggac aagcccgaga ucuccagcau ggcgguguac     1080

cggaaggugc acaaggaccu gcugaagguc gggguggaca ucuccgacug gcccguggag     1140

uucaucgaca agcucagcuu cauccugacc cugaacaccg agaacggcga gauccgcaag     1200

cagcucaaca accgccuggu cccggaguuc gacuuccuga acgacgaccu cauccagcug     1260

aucaucgaca acaaggacag cuucgagauc aagacgaaca acaaguggca ccgguucucc     1320

cugaagacca ugaacaagcu caugcccgag augcuggagc gccccgugga gcagaugacc     1380

cugcucaacg agaugggccu ggugaagaag gacaagaagc gguucgagaa caacaaguac     1440

cugcccuaca aggagaucgc caaggacauc uucaacccgg ucgccagcaa guccgugcgc     1500

gaggcccuga agaucgucaa cgccgugcuc aagaaguacg ggcacaucga cuaccuggug     1560

aucgagaugc cccgggacaa gaaccugaag gaggagcagg acaacaucaa ggaguuccag     1620

aacaagaaca agaaggcgaa ggacgccgcc uucgaggccu ucgucaagag cgugggcucc     1680

gagcagcgcg ugaaggaggc ccucagcaag aaccggaagc ugcagaugaa gaugcgccug     1740

ugguaccagc agcaggagau cgaccccuac aacggggaga ccaucgacgc gacggaccuc     1800

aucaacaacc ccgacaaguu cgagaucgac cacaucaucc cgcaguccau cagcuucgac     1860

gacuccauca acaacaagac ccugugcuuc gccagcauga accaggucaa gggccagaag     1920

acccccuacg aguucaugcu ggaggggcac ggccagggcu acgacaaguu caaggccacc     1980

gugcucgaca acaagaacuu cgaccggaac aagcgcaaga acuaccuguu cgaggagaac     2040

guguccgaca ucgagacgcg gaagcgcuuc cugagccgga accucgucga cacccgcuac     2100

uccagccggg uggugcugaa cucccugcag gacuucuucc acgagaaguc cgccgacacc     2160

aaggucaccg ugauccgcgg gaaguucacg agcaacaugc ggaagcacug gcacaucgac     2220

aagacccgcg agaccuucca ccaccacgcg aucgacgcca gcaucaucgc cgccaccccc     2280

uuccuccgga uguggaagaa gggcgggacg aucuuccccg ugaaggucgg cgaggagucc     2340

aucgacaucg agaccgggga gauccuggac gacaagaagu ucgacaaggu gauguacgag     2400

gagccguaca acggcuucgu gagcgagauc augaacgccg acgaccgcau caaguucagc     2460

caccaggucg acaagaagau gaaccgcaag guguccgacg cgaccaucua cagcacccgg     2520

accggcaagc uggccaagga caagaaggac gccgaguaca ucguggccaa ggucaaggac     2580

aucuacuccg uggacggguu caagaaguuc aagaaggugu acgacaagga caagagcaag     2640

uuccuccugu acaaguacga cccccgcacg uucuccgagc uggagcggau caucagcgac     2700

ugccccgaca aggucgagaa ggugcagacc aacggcaagg ugaaggccgu cgacgugucc     2760

cccuucgaga uguaccgccg guaccacggg augaucaaga aguacagcaa gaagggcaac     2820

ggcccggcga ucaagcagcu caaguaccug gacaagaagc ugggguccca caucgacauc     2880

acccccgcca acgccaacgg caagcacgug auccuccaga gccugaagcc cuggcgcacc     2940

gacgucuacc ugaaccacga gacgggggag uacgagauca ugggcaucaa guacuccgac     3000

cucaaguuca acaagaacgu gggguacggc aucaagaagg acaaguaccu ggagaucaag     3060

aagaacgagg aggugagcga gaaguccgag uucauguuca gccuguaccg gaaggaccgc     3120

guccggaucc agaacaugaa gaccggcgag uccguggagc uccuguucug gagccgcaac     3180

uucuccaaca agaaguacgc cgagcugaag cccaucuuca aguucgugac cgaggacgac     3240

gacuggccgg ucuacgggca cgccaagacc cagauccuca agcggcuggu gcccaaggac     3300

ugcaagaucu ggaaggugaa cacgaccauc cugggcgacc ccuacuaccu cgagaaggag     3360

agcgacaacc cgaaggacau ccuggacuga                                      3390


<210>  1656
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1656
augaccaacg gcaagauccu ggggcucgac aucggcaucg ccagcguggg ggucggcauc       60

aucgaggcca agaccgggaa gguggugcac gcgaacuccc gccuguucag cgccgccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcuccc gccggcugaa ccgccggaag      180

aagcaccgcg ucaagcgggu gcgcgaccuc uucgagaagu acgggaucgu gacggacuuc      240

cggaaccuga accugaaccc cuacgagcuc cgcgucaagg gccugaccga gcagcugacc      300

aacgaggagc uguucgcggc ccuccggacc aucagcaagc gccgggggau cuccuaccug      360

gacgacgccg aggacgacag cacgggcucc accgacuacg ccaagagcau cgacgagaac      420

cgccggcucc ugaagaccaa gaccccgggg cagauccagc uggagcgccu cgagaaguac      480

ggccagcugc ggggcaacuu cacgguguac gacgagaacg gggaggcgca ccgccugauc      540

aacguguucu ccaccagcga cuacgagaag gaggcccgga agauccucga gacccaggcc      600

gacuacaaca agaagaucac cgccgaguuc aucgacgacu acgucgagau ccugacgcag      660

aagcgcaagu acuaccacgg ccccgggaac gagaaguccc gcaccgacua cggccgguuc      720

cgcaccgacg gcaccacgcu ggagaacauc uucgggaucc ucaucggcaa gugcagcuuc      780

uaccccgacg aguaccgggc cuccaaggcg agcuacaccg cccaggagua caacuuccug      840

aacgaccuga acaaccucaa ggugcccacc gagaccggga agcuguccac ggagcagaag      900

gaggcccugg uggaguucgc caagagcacc gccacccucg gcccggcgaa gcugcugaag      960

gagaucgcca agauccucga cugcaagguc gacgagauca agggguaccg cgaggacgac     1020

aagggcaagc ccgaccugca caccuucgag cccuaccgga agcugaaguu caaccucgac     1080

uccgugaaca ucgacgaccu gagccgcgag gugcuggaca agcucgccga cauccugacg     1140

cugaacaccg agcgggaggg caucgaggac gccaucaagc gcaaccuccc caaccaguuc     1200

accgaggagc agaucuccga gaucaucaac guccgcaaga gccagagcac cgccuucaac     1260

aagggguggc acuccuucag cgcgaagcug augaacgagc ugaucccgga gcucuacgcc     1320

acguccgacg agcagaugac cauccugacc cggcuggaga aguucaaggu gaagaagaag     1380

uccagcaaga acaucaagac caucgacgag aaggagauca cggacgagau cuacaacccc     1440

guggucgcca agagcgugcg gcagaccauc aagaucauca acgccgccgu gaagaaguac     1500

ggcgacuucg acaagaucgu caucgagaug ccccgcgaca agaacgcgga cgacgagaag     1560

aaguucaucg acaagcggaa caaggagaac aagaaggaga aggacgacgc ccucaagcgc     1620

gccgccuacc uguacaacgg gaccgacaag cugcccgacg agguguucca cgagaacaag     1680

cagcucgaga ccaagauccg gcugugguac cagcagggcg agcgcugccu guacuccggg     1740

aagccgaucc ccauccagga gcuggugcac aacagcaaca acuucgagau cgaccacauc     1800

cucccccugu cccugagcuu cgacgacucc cucgcgaaca agguccuggu guacagcugg     1860

acgaaccagg agaagggcca gcggaccccc uaccagguca ucgacuccau ggacgccgcc     1920

uggagcuucc gcgagaugaa ggacuacgug cugaagcaga agggccucgg gaagaagaag     1980

cgggacuacc ugcugaccac cgagaacauc gacaagaucg aggugaagaa gaaguucauc     2040

gagcgcaacc ucgucgacac ccgcuacgcc ucccgggugg ugcugaacag ccugcagucc     2100

gcccuccgcg agcugggcaa ggacacgaag gucagcgugg ugcgggggca guucaccucc     2160

cagcugcgcc ggaaguggaa gaucgacaag agccgcgaga ccuaccacca ccacgcgguc     2220

gacgcccuca ucaucgccgc cagcucccag cugaagcugu gggagaagca ggacaacccg     2280

auguucgugg acuacggcaa caaccaggug gucgacaagc agaccgggga gauccucagc     2340

guguccgacg acgaguacaa ggagcuggug uuccagccgc ccuaccaggg cuucgucaac     2400

acgaucagcu ccaagggcuu cgaggacgag auccuguuca gcuaccaggu ggacuccaag     2460

uacaaccgga aggugagcga cgccaccauc uacuccaccc gcaaggcgaa gaucgggaag     2520

gacaagaagg aggagaccua cguccucggc aagaucaagg acaucuacag ccagaacggg     2580

uucgacacgu ucgugaagaa guacaacaag gacaagaccc aguuccugau guaccagaag     2640

gacccccuga ccugggagaa cgugaucgag gucauccucc gggacuaccc caccacgaag     2700

aaguccgagg acggcaagaa cgacgugaag ugcaaccccu ucgaggagua ccgccgggag     2760

aacggccuga ucugcaagua cagcaagaag gggaagggca cccccaucaa gucccugaag     2820

uacuacgaca agaagcucgg gaacugcauc gacaucacgc cccaggagag caagaaccgc     2880

gugguccugc ggcagaucuc cccguggcgc gccgacaucu acuucaaccc cgagacccug     2940

aaguacgagc ucaugggccu gaaguacagc gaccuguccu ucgagaaggg gaccggcaag     3000

uaccacauca gccaggagaa guacgacgcc aucaaggaga aggagggcgu ggggaaggac     3060

agcgaguuca aguucacccu cuaccggaac gaccugaucc ugaucaagga cacccucaac     3120

aacugcgagc gcaugcugcg guucggcucc aagaacgaca ccagcaagca cuacguggag     3180

cugaagcccc ucgagaaggg gacguucgac uccgaggagg agauccugcc cguccugggc     3240

aagguggcca agagcgggca guucaucaag ggccucaaca agccgaacau cuccaucuac     3300

aaggugcgca ccgacguccu gggcaacaag uacuucguga agaaggaggg ggacaagccc     3360

aagcuggacu ucaagaacaa caagaaguga                                      3390


<210>  1657
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1657
augagcaacg gcaagauccu ggggcucgac aucggcgugg ccuccgucgg ggugggcauc       60

aucgacagca agaccgggaa cgugauccac gccaacuccc gccuguucag cgcggccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcgccc gccggcugac ccgccggaag      180

aagcaccgcg ucaagcgggu gcgcgaccuc uucgagaagu acgacaucuc cacggacuuc      240

cggaaccuga accugaaccc cuacgagcuc cgcgugaagg ggcugaccga gcagcugacc      300

aacgaggagc uguucgcggc ccuccggacc aucgccaagc gccggggcau cagcuaccug      360

gacgacgccg aggacgacuc cacggggagc uccgacuacg ccaagagcau cgacgagaac      420

cgccggcucc ugaagaccaa gaccccgggc cagauccagc uggagcgccu cgagaaguac      480

gggcagcugc ggggcaacuu caccgucuac gacgagaacg gcgaggcgca ccgccugauc      540

aacguguucu ccacgagcga cuacaagaac gaggcccgga agauccucga gacccagucc      600

aacuacaaca agcagaucac cgacgaguuc aucgaggacu acaucgagau ccugacccag      660

aagcgcaagu acuaccacgg gcccggcaac gagaaguccc gcacggacua cgggcgguuc      720

cgcaccgacg gcaccacccu ggagaacauc uucggcaucc ucaucgggaa gugcagcuuc      780

uaccccgagg aguaccgggc cagcaaggcc uccuacacgg cgcaggaguu caacuuccug      840

aacgaccuga acaaccucaa ggugcccacc gagaccggca agcugagcac cgagcagaag      900

gaguaccugg ucgacuucgc caagaagucc aaggcccucg gggccagcaa gcugcugaag      960

gagaucgcca agaucgugga cugcagcgug gacgacauca agggcuaccg cgucgacaac     1020

aaggacaagc cggaccucca cacguucgag cccuaccgga agcugaaguu caaccugucc     1080

agcaucgaca ucgacgagcu cucccgcgag acccuggaca agcuggcgga cauccucacc     1140

cugaacaccg agcgggaggg gaucgaggac gccaucaagc gcaaccugcc cagccaguuc     1200

acggaggagc agaucuccga gaucgugcag auccggaaga accagagcuc cgccuucaac     1260

aagggcuggc acagcuucuc cgccaagcuc augaacgagc ugauccccga gcuguacgug     1320

accagcgagg agcagaugac cauccucacc cgccuggaga aguucaaggu caacaagaag     1380

uccagcaaga acacgaagac caucgacgag aaggagauca ccgacgagau cuacaacccg     1440

gugguggcca aguccguccg gcagaccauc aagaucauca acgcggccgu gaagaaguac     1500

ggcgacuucg acaagaucgu gaucgagaug ccccgcgaca agaacgccga ggacgagaag     1560

aaguucaucg acaagaagca gaaggagaac aagaaggaga aggacgacag ccugaagcgg     1620

gccgccuucc ucuacaacgg gacggacaac cugcccgacg gcgucuucca cgggaacaag     1680

gagcugaaga ccaagauccg ccucugguac cagcagggcg agcggugccu guacuccggg     1740

aagcugauca gcauccacga ccuggugcac aacuccaaca aguucgagau cgaccacauc     1800

cucccccuga gccuguccuu cgacgacagc cucgcgaaca aggugcuggu cuacgccugg     1860

accaaccagg agaagggcca gaagaccccg uaccagguga ucgacagcau ggacacggcc     1920

ugguccuucc gcgagaugaa ggacuacguc cugaagcaga agggccucgg gaagaagaag     1980

ugcgaguacc ugcugaccac cgagaacauc gacaagaucg aggugaagaa gaaguucauc     2040

gagcggaacc ucguggacac ccgcuacgcc agccgggucg ugcugaacuc ccugcagacc     2100

gcgcucaagg agcugggcaa ggacacgaag gugagcgucg ugcgcgggca guucaccucc     2160

cagcugcgcc ggaaguggaa caucgacaag agccgcgaga ccuaccacca ccacgccgug     2220

gacgcccuca ucaucgccgc cuccagccag cugaagcugu ggcagaagca ggagaacccc     2280

auguucgagu ccuacggcga gaaccagguc gugaacaagg agaccgggga gauccucagc     2340

aucuccgacg acaaguacaa ggagcuggug uuccagccgc ccuaccaggg cuucgucaac     2400

acgaucagcu ccaagggcuu cgaggacgag auccuguuca gcuaccaggu ggacuccaag     2460

uucaaccgga aggugagcga cgcgaccauc uacuccaccc gcaaggccaa gcucgggaag     2520

gacaagaagg acgagaccua cguccugggc aagaucaagg acaucuacag ccaggacggg     2580

uucgacacgu ucaucaagcg guacaagaag gacaagaccc aguuccugau guaccagaag     2640

gacccccuca ccugggagaa cgugaucgag gugauccugc gcgacuaccc cagcgagaag     2700

cuguccgagg acggcaagaa gaccgucaag ugcaaccccu ucgaggagua ccggcgcgag     2760

aacggccuca ucugcaagua cagcaagaag gggaacggca cgcccaucaa gucccugaag     2820

uacuacgaca agaagcuggg gaacugcauc gacaucaccc cggagaaguc caagaaccgg     2880

guggugcucc gccagaucag ccccuggcgg gccgacaucu acuucaaccu ggagacccug     2940

aaguacgagc ucaugggccu gaaguacagc gaccuguccu ucgagaaggg gaccggcaag     3000

uaccacauca gccaggagaa guacgacgcc auccgcgaga aggagggcau cgggaagaag     3060

uccgaguuca aguucacgcu cuaccggaac gaccugaucc ugaucaagga cacccucaac     3120

aacugcgagc gcaugcugcg guucggcagc aagaacgaca ccuccaagca cuacgucgag     3180

cugaagcccc ucgagaaggg gaccuucgac agcgaggagg agauccugcc cgugcugggc     3240

aagguggcca aguccgggca guucaucaag ggccucaaca agccgaacau cagcaucuac     3300

aagguccgca cggacgugcu gggcaacaag uucuucauca agaaggaggg ggacaagccc     3360

aagcuggacu ucaagaacaa caacaaguga                                      3390


<210>  1658
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1658
augagcaacg gcaagauccu ggggcucgac aucggcgugg ccuccgucgg ggugggcauc       60

aucgacgcca agaccgggaa cgugauccac gcgaacagcc gccuguucuc cgccgccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcgcgc gccggcugac ccgccggaag      180

aagcaccgcg ucaagcgggu gcgcgaccuc uucgagaagu acgacaucag cacggacuuc      240

cggaaccuga accugaaccc cuacgagcuc cgcgugaagg ggcugaccga gcagcugacc      300

aacgaggagc uguucgccgc ccuccggacc aucgccaagc gccggggcau cuccuaccug      360

gacgacgcgg aggacgacag cacggggucc agcgacuacg ccaaguccau cgacgagaac      420

cgccggcucc ugaagaccaa gaccccgggc cagauccagc uggagcgccu cgagaaguac      480

gggcagcugc ggggcaacuu caccgucuac gacgagaacg gcgaggccca ccgccugauc      540

aacguguuca gcacguccga cuacaagaac gaggcccgga agauccucga gacccagagc      600

aacuacaaca agcagaucac cgacgaguuc aucgaggacu acaucgagau ccugacccag      660

aagcgcaagu acuaccacgg gcccggcaac gagaaguccc gcacggacua cgggcgguuc      720

cgcaccgacg gcaccacccu ggagaacauc uucggcaucc ucaucgggaa gugcagcuuc      780

uaccccgagg aguaccgggc cuccaaggcg agcuacacgg cccaggaguu caacuuccug      840

aacgaccuga acaaccucaa ggugcccacc gagaccggca agcuguccac cgagcagaag      900

gaguaccugg ucgacuucgc caagaagucc aaggcccucg gggccagcaa gcugcugaag      960

gagaucgcga agaucgugga cugcagcgug gacgacauca agggcuaccg cgucgacaac     1020

aaggacaagc cggaccucca cacguucgag cccuaccgga agcugaaguu caaccugagc     1080

uccaucgaca ucgacgagcu cagccgcgag acccuggaca agcuggccga cauccucacc     1140

cugaacaccg agcgggaggg gaucgaggac acgaucaagc gcaaccugcc cucccaguuc     1200

accgaggagc agaucagcga gaucgugcag auccggaaga accaguccag cgccuucaac     1260

aagggcuggc acuccuucag cgccaagcuc augaacgagc ugauccccga gcuguacgug     1320

accuccgagg agcagaugac cauccucacg cgccuggaga aguucaaggu caacaagaag     1380

uccagcaaga acaccaagac caucgacgag aaggagauca ccgacgagau cuacaacccg     1440

gugguggcca agagcguccg gcagacgauc aagaucauca acgcggccgu gaagaaguac     1500

ggcgacuucg acaagaucgu gaucgagaug ccccgcgaca agaacgccga ggacgagaag     1560

aaguucaucg acaagaagga gaaggagaac aagaaggaga aggacgacuc ccugaagcgg     1620

gccgccuucc ucuacaacgg gaccgacaac cugcccgacg gcgucuucca cgggaacaag     1680

gagcugaaga ccaagauccg ccucugguac cagcagggcg agcggugccu guacagcggg     1740

aagcugaucu ccauccacga ccuggugcac aacagcaaca aguucgagau cgaccacauc     1800

cucccccugu cccugagcuu cgacgacucc cucgcgaaca aggugcuggu cuacgccugg     1860

accaaccagg agaagggcca gaagacgccg uaccagguga ucgacagcau ggacgccgcc     1920

uggagcuucc gcgagaugaa ggacuacguc cugaagcaga agggcaucgg gaagaagaag     1980

cgggaguacc uccugaccac cgagaacauc gacaagaucg aggugaagaa gaaguucauc     2040

gagcgcaacc ugguggacac ccgguacgcg ucccgcgucg ugcucaacag ccugcagacc     2100

gcccugaagg agcucggcaa ggacacgaag guguccgucg ugcgcgggca guucaccagc     2160

cagcugcggc gcaaguggaa gaucgacaag ucccgggaga ccuaccacca ccacgccgug     2220

gacgcccuga ucaucgccgc gagcggccag cucaagcugu ggcagaagca cgagaaccug     2280

auguucgaga acuacgggga gaaccagguc gugaacaagg agaccggcga gauccucucc     2340

aucagcgacg acgaguacaa ggagcuggug uuccagccgc ccuaccaggg cuucgucaac     2400

acgaucucca gcaagaccuu cgaggacgag auccuguucu ccuaccaggu ggacagcaag     2460

uucaaccgca agguguccga cgccaccauc uacagcaccc ggaaggccaa gcucgggaag     2520

gacaagaagg aggagacgua cguccugggc aagaucaagg acaucuacac ccaggacggg     2580

uucgacaccu ucaucaagcg cuacaagaag gacaagaccc aguuccugau guaccagaag     2640

gacccccuca cgugggagaa cgugaucgag gugauccugc gggacuaccc cuccgagaag     2700

cugagcgagg acggcaagaa gaccgucaag ugcaaccccu ucgaggagua ccgccgggag     2760

aacggccuca ucugcaagua cuccaagaag gggaacggca cccccaucaa gagccugaag     2820

uacuacgaca agaagcuggg gaacugcauc gacaucaccc ccgagaaguc caagaaccgc     2880

guggugcucc ggcagaucag cccguggcgc gccgacaucu acuucaaccu ggagacgcug     2940

aaguacgagc ucaugggccu gaaguacagc gaccuguccu ucgagaaggg gaccggcaag     3000

uaccacauca gccaggagaa guacgacgcc auccgggaga aggagggcau cgggaagaag     3060

uccgaguuca aguucacccu cuaccgcaac gaccugaucc ugaucaagga cacccucaac     3120

aacugcgagc ggaugcugcg cuucggcagc aagaacgaca cguccaagca cuacgucgag     3180

cugaagcccc ucgagaaggg gaccuucgac agcgaggagg agauccugcc cgugcugggc     3240

aagguggcga aguccgggca guucaucaag ggccucaaca agcccaacau cagcaucuac     3300

aagguccgga ccgacgugcu gggcaacaag uucuucauca agaaggaggg ggacaagccg     3360

aagcuggacu ucaagaacaa caacaaguga                                      3390


<210>  1659
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1659
augaacaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucgag       60

aacaacauca uccucgggau gggcucccgc guguuccccg agggggucgu gaaccugggc      120

gagggcgagg ggcgggagac cagcaagaac gccucccgca cggaggcgcg gggcgugcgc      180

cggcagaucu uccgccggcg ccugcggaag cgcuaccucc ugaaggagcu ggccaagcac      240

aaccucugcc cgaucagccc cacccugauc aaggacugga acgggaccga caucuucaag      300

aacgagcuga ucauccagug guuccagcuc aaccccuacg agcugcgggc caaggccauc      360

caggaggaga ucacccugau cgagcucggc cgcaucuucu accacaugau ccagcggcgc      420

ggguuccugu ccaacagccg guccgccggc gcggacguca aggagacgag caccaucuuc      480

aagggcgacg ccaagaucgg gaagaucggc aucuccgaca cccucaagag caucgagcag      540

aacaagaccc ucggguccuu ccugcacagc auccagccca aggagaacga gccguucucc      600

ggcgagcugg agcgcauccg gaaccgcuac acgacccggc agauguacau cgacgaguuc      660

gagaccaucu gggaguucca gaagcaguac cacagcgagc ucaacuccaa ccugaagacc      720

auccugggcg ggcgcaagaa ggacaacuac aaggaggacg gcgugcuguu ccaccagcgg      780

ccccugcgca gccagaagca ccuggugggg uucugcuccu ucgagcccaa gaagacgaag      840

ugcaagcuca gcgccauccc cuucgagcug uuccgcaucu accagugggu caacacccug      900

uccuacgacg ugaacggcgu gcggcagaag aucacccagg aggagcgcaa gaagaucauc      960

gaccuccugc ugagcaagga gaagccgagc uucaaggaga uccggaaggc caucgggaag     1020

cucgacggcu ccuaccaguu caacuacaag gacgacgaca agaucgucgg cagccacacc     1080

aucuccaacc ugagcaacaa gaaguucuuc gggaacaagu gguucgacuu cuccgagaag     1140

gagcaggaga acaucuggca cauccugagc uucuucgacg accgcgagaa gcucaaggag     1200

uacgcgauca agaacugguc cuucgacgag gagcgggccg ugaagaucag caaguucaac     1260

cugaaggacg gcuacuccaa ccugagccgc aaggccaucc ucaacauccu gcccuuccug     1320

cagaaggggu ucauguacga cguggccguc ggccucgccg gggugaagaa cacgcugucc     1380

gugaacuggg aggagcacga gcaguucguc cuggacaacg ugcccgagau cgugcggagc     1440

aaccucaagg gcggcuacau cgagccccug aagaacaugc ugaagaacga guucaacugc     1500

aacgacaagc agcucgagaa gcuguaccac cacuccagcg cgaucgagac cgagaacauc     1560

cuggaccgcc ugccggucaa ccuccacgcc gaccgggaga uccagaagau caagaacccc     1620

guggugauca ccgcccuguu cgagauccgc aagcugguca acgaggugau cgacaccuac     1680

gggaagccca acgagaucaa gguggagcuc gcccgggacc ugaaggccuc caagagcaac     1740

cgccuggagg cgcggaagcg ccagaaggac cucgagaagc agaacgaccg ggucaagcug     1800

gagcuggaga accucaacca gcgcuccaac cacaucaaca uccugaagua caagcugugg     1860

gaggagugca accacacgug ccccuucacc ggccagauca ucaccaucag ccagcuguuc     1920

accggggagg ugcagaucga gcacaucuuc ccgugguccc ggagccugaa cgacagcuuc     1980

aacaacaaga cgcugugcuu cgccgacgag aaccgcgcca agggcaacaa gacccccuac     2040

gagcucuacu ccgagcaggg ggaccagaag ugggaggcca ucaagcugca ggcccugagc     2100

ugcuucaaga ccaaggcgaa cuaccccaac ugcuacaaca aguucaagca cuucgucaag     2160

aagaagcacg acgaggacuu caucucccgg cagcucaacg acacccgcua caucagcaag     2220

gaggccaaga acuaccuguc caagaucugc agcaacgugc ugguggcccc cggccagaug     2280

acggccaccc uccgccacca cuggggccug aacuccaucc ugaaccucga ggacgacacc     2340

aagacccggg acgaccaccg ccaccacgcc aucgacgcgc uggucauggc cugcagcacg     2400

cggucccacc uccaggagcu cagcaagcgc aaccgguaca acaagaacua cgaccugaag     2460

gacuucccgg accccuggca gaacuuccgc caggacgccg agaacgccgu ggcgcagauc     2520

cugguguccc acaagaaguc caagagccuc cugaccgucc ggacccacaa gaccaucaag     2580

aagggggugg agcacaagaa caucggcgug gccgcccgcg ggcagcugca caaggagagc     2640

gucuacggcc uccggaaggc ccccaacucc aacaaggccu uccacauccg caagccccug     2700

gagagccuga cgaccgagaa gcagcucgag aagaucgugg acgacuccau ccggcugcug     2760

auccucaagc gcaucaacau caucggcggg uucaucaagg gcaagauccc gaaggagacc     2820

uucuucgugg ucgacgagaa cgggaucaag cagccccaga ucuuccugcc caaccggaac     2880

ggcaaccccg ugccgaucaa gaaggugcgc auccgggaga acaucggggg cgcggagaag     2940

cugaaggaga acaccaacca guacgucaac ccccgcagca accaccacgu gaucguguac     3000

gaggacagca acggcgggcu caaggacgac gucgugaccu ucuggacggc cguggagcgg     3060

aagcgccagg gccaggacgu cuucaagcug cccaacgacg gggagaagau cguguccacc     3120

cugcagauca acgacauguu ccuccugggc cugaacgacg acgacaucau cacccucacc     3180

agcaaggagc ugaagaagca ccuguucaag gugcagacgc ucuccagcuc cuucuacgag     3240

uuccggcuga gcaccgacuc cacccagaac aagaacacca acagcacggu cuuccgccgg     3300

auccaguccu ucgggauggg caagaccggc uggaaggccu ucaacccgau caaggugaag     3360

cugaacagca ucggggaggu gaagaucauc uga                                  3393


<210>  1660
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1660
augaccaacg gcaagauccu ggggcucgac aucggcaucg ccagcguggg ggucggcauc       60

aucgaggcca agaccgggaa gguggugcac gcgaacuccc gccuguucag cgccgccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcuccc gccggcugaa ccgccggaag      180

aagcaccgcg ucaagcgggu gcgcgaccuc uucgagaagu acgggaucgu gacggacuuc      240

cggaaccuga accugaaccc cuacgagcuc cgcgucaagg gccugaccga gcagcugacc      300

aacgaggagc uguucgcggc ccuccggacc aucagcaagc gccgggggau cuccuaccug      360

gacgacgccg aggacgacag cacgggcucc accgacuacg ccaagagcau cgacgagaac      420

cgccggcucc ugaagaccaa gaccccgggg cagauccagc uggagcgccu cgagaaguac      480

ggccagcugc ggggcaacuu cacgguguac gacgagaacg gggaggcgca ccgccugauc      540

aacguguucu ccaccagcga cuacgagaag gaggcccgga agauccucga gacccaggcc      600

gacuacaaca agaagaucac cgccgaguuc aucgacgacu acgucgagau ccugacgcag      660

aagcgcaagu acuaccacgg ccccgggaac gagaaguccc gcaccgacua cggccgguuc      720

cgcaccgacg gcaccacgcu ggagaacauc uucgggaucc ucaucggcaa gugcagcuuc      780

uaccccgacg aguaccgggc cuccaaggcg agcuacaccg cccaggagua caacuuccug      840

aacgaccuga acaaccucaa ggugcccacc gagaccggga agcuguccac ggagcagaag      900

gaggcccugg uggaguucgc caagagcacc gccacccucg gcccggcgaa gcugcugaag      960

gagaucgcca agauccucga cugcaagguc gacgagauca agggguaccg cgaggacgac     1020

aagggcaagc ccgaccugca caccuucgag cccuaccgga agcugaaguu caaccucgac     1080

uccgugaaca ucgacgaccu gagccgcgag gugcuggaca agcucgccga cauccugacg     1140

cugaacaccg agcgggaggg caucgaggac gccaucaagc gcaaccuccc caaccaguuc     1200

accgaggagc agaucuccga gaucaucaac guccgcaaga gccagagcac cgccuucaac     1260

aagggguggc acuccuucag cgcgaagcug augaacgagc ugaucccgga gcucuacgcc     1320

acguccgacg agcagaugac cauccugacc cggcuggaga aguucaaggu gaagaagaag     1380

uccagcaaga acaucaagac caucgacgag aaggagauca cggacgagau cuacaacccc     1440

guggucgcca agagcgugcg gcagaccauc aagaucauca acgccgccgu gaagaaguac     1500

ggcgacuucg acaagaucgu caucgagaug ccccgcgaca agaacgcgga cgacgagaag     1560

aaguucaucg acaagcggaa caaggagaac aagaaggaga aggacgacgc ccucaagcgc     1620

gccgccuacc uguacaacgg gaccgacaag cugcccgacg agguguucca cggcaacaag     1680

cagcucgaga ccaagauccg gcugugguac cagcaggggg agcgcugccu guacuccggc     1740

aagccgaucc ccauccagga gcuggugcac aacagcaaca acuucgagau cgaccacauc     1800

cucccccugu cccugagcuu cgacgacucc cucgcgaaca agguccuggu guacagcugg     1860

acgaaccagg agaagggcca gcggaccccc uaccagguca ucgacuccau ggacgccgcc     1920

uggagcuucc gcgagaugaa ggacuacgug cugaagcaga aggggcucgg caagaagaag     1980

cgggacuacc ugcugaccac cgagaacauc gacaagaucg aggugaagaa gaaguucauc     2040

gagcgcaacc ucgucgacac ccgcuacgcc ucccgggugg ugcugaacag ccugcagucc     2100

gcccuccgcg agcuggggaa ggacacgaag gucagcgugg ugcggggcca guucaccucc     2160

cagcugcgcc ggaaguggaa gaucgacaag agccgcgaga ccuaccacca ccacgcgguc     2220

gacgcccuca ucaucgccgc cagcucccag cugaagcugu gggagaagca ggacaacccg     2280

auguucgugg acuacgggaa caaccaggug gucgacaagc agaccggcga gauccucagc     2340

guguccgaca acgaguacaa ggagcuggug uuccagccgc ccuaccaggg cuucgucaac     2400

acgaucagcu ccaagggguu cgaggacgag auccuguuca gcuaccaggu ggacuccaag     2460

uacaaccgga aggugagcga cgccaccauc uacuccaccc gcaaggcgaa gaucggcaag     2520

gacaagaagg aggagaccua cguccucggg aagaucaagg acaucuacag ccagaacggc     2580

uucgacacgu ucgugaagaa guacaacaag gacaagaccc aguuccugau guaccagaag     2640

gacccccuga ccugggagaa cgugaucgag gucauccucg gcgacuaccc caccacgaag     2700

aaguccgagg acgggaagaa cgacgugaag ugcaaccccu ucgaggagua ccggcgcgag     2760

aacggccuga ucugcaagua cagcaagaag gggaagggca cccccaucaa gucccugaag     2820

uacuacgaca agaagcucgg gaacugcauc gacaucaccc ccgaggagag ccggaacaag     2880

gugauccugc aguccaucaa cccguggcgc gccgacgucu acuucaaccc cgagacccug     2940

aaguacgagc ucaugggccu gaaguacagc gaccuguccu ucgagaaggg cacggggaac     3000

uaccacauca gccaggagaa guacgacgcc aucaaggaga aggagggcau cgggaagaag     3060

uccgaguuca aguucacccu cuaccggaac gaccugaucc ugaucaagga caucgccagc     3120

ggcgagcagg agaucuaccg cuuccucagc cggaccaugc ccaacgugaa ccacuacgug     3180

gagcugaagc ccuacgacaa ggagaaguuc gacaacgucc aggagcuggu ggaggcccuc     3240

ggggaggcgg acaagguggg ccgcugcauc aagggccuga acaagccgaa caucuccauc     3300

uacaaggucc ggaccgacgu gcuggggaac aaguacuucg ugaagaagga gggcgacaag     3360

cccaagcucg acuucaagaa caacaagaag uga                                  3393


<210>  1661
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1661
augaccaacg gcaagauccu ggggcucgac aucggcaucg ccagcguggg ggucggcauc       60

aucgaggcca agaccgggaa gguggugcac gcgaacuccc gccuguucag cgccgccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcuccc gccggcugaa ccgccggaag      180

aagcaccgcg ucaagcgggu gcgcgaccuc uucgagaagu acgagaucgu gacggacuuc      240

cggaaccuga accugaaccc cuacgagcuc cgcgucaagg ggcugaccga gcagcugacc      300

aacgaggagc uguucgcggc ccuccggacc aucagcaagc gccggggcau cuccuaccug      360

gacgacgccg aggacgacag cacggggucc accgacuacg ccaagagcau cgacgagaac      420

cgccggcucc ugaagaccaa gaccccgggc cagauccagc uggagcgccu cgagaaguac      480

gggcagcugc ggggcaacuu cacgguguac gacgagaacg gcgaggcgca ccgccugauc      540

aacguguucu ccaccagcga cuacgagaag gaggcccgga agauccucga gacccaggcc      600

gacuacaaca agaagaucac cgccgaguuc aucgacgacu acgucgagau ccugacgcag      660

aagcgcaagu acuaccacgg gcccggcaac gagaaguccc gcaccgacua cgggcgguuc      720

cgcaccgacg gcaccacgcu ggagaacauc uucggcaucc ucaucgggaa gugcagcuuc      780

uaccccgacg aguaccgggc cuccaaggcg agcuacaccg cccaggagua caacuuccug      840

aacgaccuga acaaccucaa ggugcccacc gagaccggca agcuguccac ggagcagaag      900

gaggcccugg uggaguucgc caagagcacc gccacccucg ggccggcgaa gcugcugaag      960

gagaucgcca agauccucga cugcaagguc gacgagauca agggcuaccg cgaggacgac     1020

aaggggaagc ccgaccugca caccuucgag cccuaccgga agcugaaguu caaccucgac     1080

uccgugaaca ucgacgaccu gagccgcgag gugcuggaca agcucgccga cauccugacg     1140

cugaacaccg agcgggaggg caucgaggac gccauccgcc acaaccuccc caaccaguuc     1200

accgagggcc agaucuccga gaucaucaag guccgcaaga gccagagcac cgccuucaac     1260

aagggguggc acuccuucag cgcgaagcug augaacgagc ugaucccgga gcucuacgcc     1320

acguccgacg agcagaugac cauccugacc cggcuggaga aguucaaggu gaacaagaag     1380

uccagcaaga acaccaagac gaucgacgag aaggagguga ccgacgagau cuacaacccc     1440

gucguggcca agagcgugcg gcagaccauc aagaucauca acgccgccgu caagaaguac     1500

ggcgacuucg acaagaucgu gaucgagaug ccccgcgaca agaacgcgga cgacgagaag     1560

aaguucaucg acaagcggaa caaggagaac aagaaggaga aggacgacgc ccucaagcgc     1620

gccgccuacc uguacaacgg gaccgacaag cugcccgacg agguguucca cggcaacaag     1680

cagcucgaga cgaagauccg gcugugguac cagcaggggg agcgcugccu guacuccggc     1740

aagccgaucc ccauccagga gcugguccac aacagcaaca acuucgagau cgaccacauc     1800

cucccccugu cccugagcuu cgacgacucc cucgcgaaca aggugcuggu cuacgccugg     1860

accaaccagg agaagggcca gaagaccccc uaccagguga ucgacagcau ggacgccgcc     1920

ugguccuucc gggagaugaa ggacuacgug cugaagcaga aggggcucgg caagaagaag     1980

cgcgacuacc ugcugaccac cgagaacauc gacaagaucg aggucaagaa gaaguucauc     2040

gagcggaacc ucguggacac gcgcuacgcc agccgcgugg uccugaacuc ccugcagagc     2100

gcgcuccggg agcuggggaa ggacaccaag aucuccguga uccgcggcca guucaccagc     2160

cagcugcggc gcaaguggaa gaucgacaag ucccgggaga ccuaccacca ccacgccgug     2220

gacgcccuca ucaucgccgc cagcagccag cugaagcugu gggagaagca ggacaacccg     2280

auguucgucg acuacgggaa caaccaggug guggacaagc agacgggcga gauccucucc     2340

gucagcgacg acgaguacaa ggagcuggug uuccagccgc ccuaccaggg cuucgugaac     2400

augaucucca gcaagggguu cgaggacgag auccuguucu ccuaccaggu cgacagcaag     2460

uacaaccgca agguguccga cgcgaccauc uacagcaccc ggaaggccaa gaucggcaag     2520

gacaagaagg aggagaccua cgugcucggg aagaucaagg acaucuacuc ccagaacggc     2580

uucgacacgu ucaucaagaa guacaacaag gacaagaccc aguuccugau guaccagaag     2640

gacccccuga ccugggagaa cgucaucgag gugauccucc gcgacuaccc caccacgaag     2700

aaguccgagg acggcaagaa cgacgugaag ugcaaccccu ucgaggagua ccggcgcgag     2760

aacgggcugg ucugcaagua cagcaagaag ggcaagggga cccccaucaa gagccugaag     2820

uacuacgaca agaagcucgg caacugcauc gacaucaccc ccgagggguc caagaacgag     2880

guggugcugc agagccugaa cccguggcgg gccgacgucu acuucaaccc cgagacccuc     2940

aaguacgagc ugcugggccu caaguacucc gaccugagcu ucgagaaggg cacggggaag     3000

uaccacaucu cccaggagaa guacgacgug aucaaggaga aggagggcau cgggaagaag     3060

uccgaguuca aguucacccu guaccgcaac gaccucaucc ugaucaagga caccgccagc     3120

ggcgagcagg agaucuaccg guuccugagc cgcaccaugc ccaacgugaa gcacuacgcc     3180

gagcucaagc ccuacgacaa ggagaaguuc gacaacgucc aggagcuggu ggaggcgcug     3240

ggggaggccg acaagguggg ccggugcauc aagggccuca acaagagcaa ccuguccauc     3300

uacaaggucc gcacggacgu gcuggggaac aaguacuucg ugaagaagga gggcgacaag     3360

ccgaagcucg acuucaagaa caacaagaag uga                                  3393


<210>  1662
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1662
augaccaacg gcaagauccu ggggcucgac aucggcaucg ccagcguggg ggucggcauc       60

aucgaggcca agaccgggaa gguggugcac gcgaacuccc gccuguucag cgccgccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcuccc gccggcugaa ccgccggaag      180

aagcaccgcg ucaagcgggu gcgcgaccuc uucgagaagu acgggaucgu gacggacuuc      240

cggaaccuga accugaaccc cuacgagcuc cgcgucaagg gccugaccga gcagcugacc      300

aacgaggagc uguucgcggc ccuccggacc aucagcaagc gccgggggau cuccuaccug      360

gacgacgccg aggacgacag cacgggcucc accgacuacg ccaagagcau cgacgagaac      420

cgccggcucc ugaagaccaa gaccccgggg cagauccagc uggagcgccu cgagaaguac      480

ggccagcugc ggggcaacuu cacgguguac gacgagaacg gggaggcgca ccgccugauc      540

aacguguucu ccaccagcga cuacgagaag gaggcccgga agauccucga gacccaggcc      600

gacuacaaca agaagaucac cgccgaguuc aucgacgacu acgucgagau ccugacgcag      660

aagcgcaagu acuaccacgg ccccgggaac gagaaguccc gcaccgacua cggccgguuc      720

cgcaccgacg gcaccacgcu ggagaacauc uucgggaucc ucaucggcaa gugcaacuuc      780

uaccccgacg aguaccgggc cagcaaggcg uccuacaccg cccaggagua caacuuccug      840

aacgaccuga acaaccucaa ggugcccacc gagaccggga agcugagcac ggagcagaag      900

gagucccugg uggaguucgc caagaacacc gccauccucg gcccggccaa gcugcugaag      960

gagaucgcga agauccucga cugcaagguc gacgagauca agggguaccg cgaggacgac     1020

aagggcaagc ccgaccugca caccuucgag cccuaccgga agcugaaguu caaccucgac     1080

agcgugaaca ucgacuaccu gucccgcgag gugcuggaca agcucgccga cauccugacc     1140

cugaacacgg agcgggaggg caucgaggac gccaucaagc gcaaccuccc caaccaguuc     1200

accgaggagc agaucagcga gaucaucaac guccggaagu cccagagcac cgccuucaac     1260

aagggguggc acagcuucuc cgccaagcug augaacgagc ugaucccgga gcucuacgcg     1320

accagcgacg agcagaugac gauccugacc cgccuggaga aguucaaggu gaagaagaag     1380

uccagcaaga acaucaagac caucgacgag aaggagauca ccgacgagau cuacaacccc     1440

guggucgcca aguccgugcg gcagacgauc aagaucauca acgccgccgu gaagaaguac     1500

ggcgacuucg acaagaucgu caucgagaug ccccgcgaca agaacgccga cgacgagaag     1560

aaguucauca acaagcggaa caaggagaac aagaaggaga aggacgacgc gcucaagcgc     1620

gccgccuacc uguacaacgg gaccgacaag cugcccgacg agguguucca cggcaacaag     1680

cagcucgaga ccaagauccg gcugugguac cagcaggggg agcgcugccu guacagcggc     1740

aagccgaucc ccauccagga gcuggugcac aacuccaaca acuucgagau cgaccacauc     1800

cucccccuga gccuguccuu cgacgacagc cucgccaaca agguccuggu guacuccugg     1860

accaaccagg agaagggcca gaagacgccc uaccagguca ucgacagcau ggacgcggcc     1920

ugguccuucc gggagaugaa ggaguacgug cugaagcaga aggggcucgg caagaagaag     1980

cgcgacuacc ugcugaccac cgagaacauc gacaagaucg aggugaagaa gaaguucauc     2040

gagcggaacc ucgucgacac ccgcuacgcc agccgcgugg ugcugaacuc ccugcagagc     2100

gcccuccggg agcuggggaa ggacaccaag gucuccgugg ugcgcggcca guucacgagc     2160

cagcugcggc gcaaguggaa gaucgacaag ucccgggaga ccuaccacca ccacgccguc     2220

gacgcgcuca ucaucgccgc cagcagccag cugaagcugu gggagaagca ggacaacccg     2280

auguucgugg acuacgggaa caaccaggug gucgacaagc agaccggcga gauccucucc     2340

gugagcgaca acgaguacaa ggagcuggug uuccagccgc ccuaccaggg cuucgucaac     2400

accaucucca gcaagggguu cgaggacgag auccuguucu ccuaccaggu ggacagcaag     2460

uacaaccgca agguguccga cgccacgauc uacagcaccc ggaaggccaa gaucggcaag     2520

gacaagaagg aggagaccua cguccucggg aagaucaagg acaucuacuc ccagaacggc     2580

uucgacaccu ucgugaagaa guacaacaag gacaagacgc aguuccugau guaccagaag     2640

gacccccuga ccugggagaa cgugaucgag gucauccucg gcgacuaccc caccaccaag     2700

aaguccgagg acgggaagaa cgacgugaag ugcaaccccu ucgaggagua ccgccgggag     2760

aacggccuga ucugcaagua cagcaagaag gggaagggca cgcccaucaa gagccugaag     2820

uacuacgaca agaagcucgg gaacugcauc gacaucaccc ccgaggaguc ccgcaacaag     2880

gugauccugc agagcaucaa cccguggcgg gcggacgucu acuucaaccc cgagacccug     2940

aaguacgagc ucaugggccu gaaguacucc gaccugagcu ucgagaaggg caccgggaac     3000

uaccacaucu cccaggagaa guacgacgcc aucaaggaga aggagggcau cgggaagaag     3060

uccgaguuca aguucacgcu cuaccgcaac gaccugaucc ugaucaagga caucgccagc     3120

ggcgagcagg agaucuaccg guuccucagc cgcaccaugc ccaacgugaa ccacuacgug     3180

gagcugaagc ccuacgacaa ggagaaguuc gacaacgucc aggagcuggu ggaggcccuc     3240

ggggaggccg acaagguggg ccggugcauc aagagccuga acaagccgaa caucuccauc     3300

uacaaggucc gcaccgacgu gcugggcaac aaguacuucg ugaagaagga gggggacaag     3360

cccaagcucg acuucaagaa caacaagaag uga                                  3393


<210>  1663
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1663
augagcaacg gcaagauccu ggggcucgac aucggcgugg ccuccgucgg ggugggcauc       60

aucgacgcca agaccgggaa ggugauccac gcgagcuccc gccuguucag cgccgccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcgcgc gccggcugac ccgccggaag      180

aagcaccgca ucaagcgggu ccgcgaccuc uucgagaagu acgagaucgu gacggacuuc      240

uccaaccuga accugaaccc cuacgagcuc cgggugaagg ggcugaccga gcagcugacc      300

aacgaggagc uguucgccgc ccuccgcacc aucgccaagc ggcgcggcau cagcuaccug      360

gacgacgcgg aggacgacuc cacggggagc uccgacuacg ccaagagcau cgaggagaac      420

cggcgccucc ugaagaacau gaccccgggc cagauccagc uggagcggcu cgagaaguac      480

gggcagcugc gcggcaacuu caccgucuac gacgagaacg gcgaggccca ccggcugauc      540

aacguguucu ccaccagcga cuacaagaac gaggcccgca agauccucga gacgcagucc      600

aacuacaaca agcagaucac cgacgaguuc aucgaggacu acaucgagau ccugacccag      660

aagcggaagu acuaccacgg gcccggcaac gagaaguccc gcaccgacua cgggcgcuac      720

cggacggacg gcaccacccu ggagaacauc uucggcaucc ucaucgggaa gugcaccuuc      780

uaccccgacg aguaccgcac gagcaaggcc agcuacaccg cgcaggagua caacuuccug      840

aacgaccuga acaaccucaa ggugcccacc gagaccggca agcuguccac ggagcagaag      900

cacuaccugg ucgacuucgc caagagcucc aaggcccucg ggguggccaa gcugcugaag      960

gagaucgcca agaucgugga gugcagcguc gacgacauca agggcuaccg ggaggacaac     1020

aaggggaagc cggaccucca caccuucgag cccuaccgca agcugaaguu caaccugagc     1080

cucaucgaca ucgacgagcu gucccgggag acccuggaca agcucgcgga cauccugacc     1140

cugaacacgg agcgcgaggg caucgaggac gccaucaagc ggaaccuccc caaccaguuc     1200

accgaggagc agaucgugga gcuggugaac guccgcaaga accagagcuc cgccuucaac     1260

aagggcuggc acagcuucuc cgugaagcug augaacgagc ucauccccga gcuguacgcc     1320

agcuccgagg agcagaugac cauccugacc cggcucgaga aguucaaggu gaacaagaag     1380

uccagcgaga acacgaagac caucaacgag aaggagauca ccgacgagau cuacaacccg     1440

gucguggcca agagcguguc ccagaccguc aagaucauca acgcggccgu gaagaaguac     1500

ggggacuucg acaagaucgu gaucgagaug ccccgcgaca agaacgccga cgacgagaag     1560

aaguucaucg acaagaagca gaaggagaac gccaaggagg ucgaggacgc ccugaagcgg     1620

gcggccuacc uguacaacgg cacggacaag cuccccgacg ggguguucca cggcaacaag     1680

cagcugaaga ccaagauccg ccugugguac cagcaggggg agcggugccu guacagcggc     1740

aagcccaucu ccauccagga ccucguccac aacagcaaca acuucgagau cgaccacauc     1800

cugccgcugu cccucagcuu cgacgacucc cuggccaaca aggugcuggu guacgccugg     1860

accaaccagg agaagggcca gaagaccccc uaccagguca ucgacagcau ggacgcggcc     1920

ugguccuucc gcgagaugaa ggacuacgug cucaaccaga aggggaucgg caagaagaag     1980

cgggaguacc ugcugacgac cgagaacauc gacaagaucg aggugaagaa gaaguucauc     2040

gagcgcaacc ucgucgacac ccgguacgcc agccgcgugg ugcugaacag cauccaggcc     2100

gcccugaagg agcucgacaa ggacaccaag gucuccguga uccgggggca guucaccagc     2160

cagcugcgcc gcaaguggaa gaucgacaag ucccgggaga cguaccacca ccacgcggug     2220

gacgcccuga ucaucgccgc cagcucccag cucaagcugu ggcagaagca ggagaacccc     2280

auguucgucg acuacggcga gaaccaggug gugaacaagg agaccgggga gauccugagc     2340

gucuccgacg acgacuacaa ggagcucgug uuccagcccc cguaccaggg cuucgugaac     2400

accaucagcu ccaaggccuu cgaggacgag auccuguuca gcuaccaggu cgacuccaag     2460

uacaaccgca aggugagcga cgcgaccauc uacuccacgc ggaaggccaa gcugggcaag     2520

gacaagaagg aggagaccua cgugcucggg aagaucaagg acaucuacag ccaggacggc     2580

uucgacgccu ucaucaagcg cuacaagaag gacaagaccc aguuccugau guaccagaag     2640

gaccccguca ccugggagaa cgugaucgag gugauccugc gggacuaccc cuccgagaag     2700

cucagcgagg acgggaagaa gaacgucaag ugcaaccccu ucgaggagua ccgccgggag     2760

aacggccuga ucugcaagua cuccaagaag ggcaagggga cgccgaucaa gagccugaag     2820

uacuacgaca agaagcucgg caacugcauc gacaucaccc ccaaggggag caagaacgag     2880

guggugcugc agucccugaa ccccuggcgc gccgaccucu acuucaaccc cgacacccug     2940

aaguacgagc ugaugggccu caaguacagc gaccuguccu ucgagaaggg gaccggcaag     3000

uaccacauca gccaggagcg guacgacgag aucaagguca aggagggcau cgggaagaac     3060

uccgaguuca aguucacgcu guaccgcaac gaccucaucc ugaucaagga caccgagagc     3120

ggcgaggagg agcuguaccg guuccucucc cgcaccaugc cgaacgugaa gcacuacgug     3180

gagcugaagc ccuacgacaa ggagcgguac gacgggggcc aggagcugau gaaggucuuc     3240

gggaacgugg ccaacggcgg ccagugccuc aagagccuga acaaguccaa ccugagcauc     3300

uacaaggugc gcaccgacgu ccucgggaac aaguucuuca ucaagaagga gggcgacaag     3360

cccaagcugg acuucaagaa caacaagaag uga                                  3393


<210>  1664
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1664
augaccaacg gcaagauccu ggggcucgac aucggcaucg ccagcguggg ggucggcauc       60

aucgaggcca agaccgggaa gguggugcac gcgaacuccc gccuguucag cgccgccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcuccc gccggcugaa ccgccggaag      180

aagcaccgcg ucaagcgggu gcgcgaccuc uucgagaagu acgggaucgu gacggacuuc      240

cggaaccuga accugaaccc cuacgagcuc cgcgucaagg gccugaccga gcagcugaag      300

aacgaggagc uguucgcggc ccuccggacc aucagcaagc gccgggggau cuccuaccug      360

gacgacgccg aggacgacag caccggcucc acggacuacg ccaagagcau cgacgagaac      420

cgccggcucc ugaagaacaa gaccccgggg cagauccagc uggagcgccu cgagaaguac      480

ggccagcugc ggggcaacuu caccguguac gacgagaacg gggaggcgca ccgccugauc      540

aacguguucu ccaccagcga cuacgagaag gaggcccgga agauccucga gacgcaggcc      600

gacuacaaca agaagaucac cgccgaguuc aucgacgacu acgucgagau ccugacccag      660

aagcgcaagu acuaccacgg ccccgggaac gagaaguccc gcaccgacua cggccgguuc      720

cgcacggacg gcaccacccu ggagaacauc uucgggaucc ucaucggcaa gugcaacuuc      780

uaccccgacg aguaccgggc cagcaaggcg uccuacaccg cccaggagua caacuuccug      840

aacgaccuga acaaccucaa ggugagcacg gagaccggga agcuguccac cgagcagaag      900

gagagccugg uggaguucgc caagaacacc gccacgcucg gccccgccaa gcugcugaag      960

gagaucgcga agauccucga cugcaagguc gacgagauca agggguaccg cgaggacgac     1020

aagggcaagc cggaccugca caccuucgag cccuaccgga agcugaaguu caaccucgag     1080

uccaucaaca ucgacgaccu gagccgcgag gugaucgaca agcuggccga cauccucacc     1140

cugaacaccg agcgggaggg caucgaggac gccaucaagc gcaaccugcc caaccaguuc     1200

acggaggagc agaucuccga gaucaucaag gugcgcaaga gccagagcac cgccuucaac     1260

aagggguggc acuccuucag cgccaagcuc augaacgagc ugauccccga gcuguacgcg     1320

accuccgacg agcagaugac cauccucacg cggcuggaga aguucaaggu caacaagaag     1380

uccagcaaga acaccaagac caucgacgag aaggagguga ccgacgagau cuacaacccg     1440

guggucgcca agagcgugcg gcagacgauc aagaucauca acgccgccgu gaagaaguac     1500

ggcgacuucg acaagaucgu caucgagaug ccccgcgaca agaacgccga cgacgagaag     1560

aaguucaucg acaagcggaa caaggagaac aagaaggaga aggacgacgc gcugaagcgc     1620

gccgccuacc ucuacaacuc cagcgacaag cugcccgacg agguguucca cgggaacaag     1680

cagcuggaga ccaagauccg gcucugguac cagcagggcg agcgcugccu guacuccggg     1740

aagcccauca gcauccagga gcuggugcac aacuccaaca acuucgagau cgaccacauc     1800

cugccgcuca gccuguccuu cgacgacagc cuggccaaca agguccucgu guacgcgugg     1860

accaaccagg agaagggcca gaagaccccc uaccagguca ucgacuccau ggacgccgcc     1920

uggagcuucc gggagaugaa ggacuacgug cugaagcaga agggccuggg gaagaagaag     1980

cgcgacuacc uccugacgac cgagaacauc gacaagaucg aggugaagaa gaaguucauc     2040

gagcggaacc uggucgacac ccgcuacgcc ucccgcgugg ugcucaacag ccugcagucc     2100

gcccugcggg agcucggcaa ggacaccaag gucagcgugg ugcgcgggca guucaccagc     2160

cagcugcggc gcaaguggaa gaucgacaag ucccgggaga cguaccacca ccacgcgguc     2220

gacgcccuga ucaucgccgc cagcucccag cucaagcugu gggagaagca ggacaacccc     2280

auguucgugg acuacggcaa gaaccaggug gucgacaagc agaccgggga gauccugagc     2340

guguccgacg acgaguacaa ggagcucgug uuccagcccc cguaccaggg cuucgucaac     2400

accaucagcu ccaagggcuu cgaggacgag auccuguuca gcuaccaggu ggacuccaag     2460

uacaaccgca aggugagcga cgccaccauc uacuccacgc ggaaggcgaa gaucgggaag     2520

gacaagaagg aggagaccua cguccugggc aagaucaagg acaucuacag ccagaacggg     2580

uucgacaccu ucaucaagaa guacaacaag gacaagaccc aguuccucau guaccagaag     2640

gacucccuga cgugggagaa cgugaucgag gugauccugc gcgacuaccc caccaccaag     2700

aaguccgagg acggcaagaa cgacgucaag ugcaaccccu ucgaggagua ccggcgcgag     2760

aacggccuca ucugcaagua cagcaagaag gggaagggca cccccaucaa gagccugaag     2820

uacuacgaca agaagcuggg gaacugcauc gacaucacgc cggaggaguc ccggaacaag     2880

gugauccucc agagcaucaa ccccuggcgc gccgacgugu acuucaaccc cgagacccug     2940

aaguacgagc ugaugggccu caaguacagc gaccuguccu ucgagaaggg gaccggcaac     3000

uaccacauca gccaggagaa guacgacgcc aucaaggaga aggagggcau cgggaagaag     3060

uccgaguuca aguucacccu guaccggaac gaccucaucc ugaucaagga caucgccagc     3120

ggcgagcagg agaucuaccg cuuccugucc cggacgaugc ccaacgucaa ccacuacgug     3180

gagcucaagc cguacgacaa ggagaaguuc gacaacgugc aggagcuggu cgaggcccug     3240

ggggaggcgg acaagguggg ccgcugcauc aaggggcuca acaagcccaa caucagcauc     3300

uacaaggugc ggaccgacgu ccugggcaac aaguacuucg ugaagaagaa gggcgacaag     3360

cccaagcugg acuucaagaa caacaagaag uga                                  3393


<210>  1665
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1665
augaccaacg gcaagauccu ggggcucgac aucggcaucg ccagcguggg ggucggcauc       60

aucgaggcca agaccgggaa gguggugcac gcgaacuccc gccuguucag cgccgccaac      120

gccgagaaca acgccgagcg gcgcggcuuc cggggcuccc gccggcugaa ccgccggaag      180

aagcaccgcg ucaagcgggu gcgcgaccuc uucgagaagu acgggaucgu gacggacuuc      240

cggaaccuga accugaaccc cuacgagcuc cgcgucaagg gccugaccga gcagcugacc      300

aacgaggagc uguucgcggc ccuccggacc aucagcaagc gccgggggau cuccuaccug      360

gacgacgccg aggacgacag cacgggcucc accgacuacg ccaagagcau cgacgagaac      420

cgccggcucc ugaagaccaa gaccccgggg cagauccagc uggagcgccu cgagaaguac      480

ggccagcugc ggggcaacuu cacgguguac gacgagaacg gggaggcgca ccgccugauc      540

aacguguucu ccaccagcga cuacgagaag gaggcccgga agauccucga gacccaggcc      600

gacuacaaca agaagaucac cgccgaguuc aucgacgacu acgucgagau ccugacgcag      660

aagcgcaagu acuaccacgg ccccgggaac gagaaguccc gcaccgacua cggccgguuc      720

cgcaccgacg gcaccacgcu ggagaacauc uucgggaucc ucaucggcaa gugcagcuuc      780

uaccccgacg aguaccgggc cuccaaggcg agcuacaccg cccaggagua caacuuccug      840

aacgaccuga acaaccucaa ggugcccacc gagaccggga agcuguccac ggagcagaag      900

gaggcccugg uggaguucgc caagagcacc gccacccucg gcccggcgaa gcugcugaag      960

gagaucgcca agauccucga cugcaagguc gacgagauca agggguaccg cgaggacgac     1020

aagggcaagc ccgaccugca caccuucgag cccuaccgga agcugaaguu caaccucgac     1080

uccgugaaca ucgacuaccu gagccgcgag gugcuggaca agcucgccgg cauccugacg     1140

cugaacaccg agcgggaggg gaucgaggac gccaucaagc gcaaccuccc caaccaguuc     1200

accgaggagc agaucuccga gaucaucaac guccgcaaga gccagagcac cgccuucaac     1260

aagggcuggc acuccuucag cgcgaagcug augaacgagc ugaucccgga gcucuacgcc     1320

acguccgacg agcagaugac cauccugacc cggcuggaga aguucaaggu gaagaagaag     1380

uccagcaaga acaucaagac caucgacgag aaggagauca cggacgagau cuacaacccc     1440

guggucgcca agagcgugcg gcagaccauc aagaucauca acgccgccgu gaagaaguac     1500

ggggacuucg acaagaucgu caucgagaug ccccgcgaca agaacgcgga cgacgagaag     1560

aaguucauca acaagcggaa caaggagaac aagaaggaga aggacgacgc ccucaagcgc     1620

gccgccuacc uguacaacgg caccgacaag cugcccgacg agguguucca cgggaacaag     1680

cagcucgaga ccaagauccg gcugugguac cagcagggcg agcgcugccu guacuccggc     1740

aagccgaucc ccauccagga gcuggugcac aacagcaaca acuucgagau cgaccacauc     1800

cucccccugu cccugagcuu cgacgacucc cucgcgaaca agguccuggu guacagcugg     1860

acgaaccagg agaaggggca gaagaccccc uaccagguca ucgacuccau ggacgccgcc     1920

uggagcuucc gggagaugaa ggaguacgug cugaagcaga agggccucgg gaagaagaag     1980

cgcgacuacc ugcugaccac cgagaacauc gacaagaucg aggugaagaa gaaguucauc     2040

gagcggaacc ucgucgacac ccgcuacgcc ucccgcgugg ugcugaacag ccugcagucc     2100

gcccuccggg agcugggcaa ggacacgaag gucagcgugg ugcgcgggca guucaccucc     2160

cagcugcggc gcaaguggaa gaucgacaag agccgggaga ccuaccacca ccacgcgguc     2220

gacgcccuca ucaucgccgc cagcucccag cugaagcugu gggagaagca ggacaacccg     2280

auguucgugg acuacggcaa caaccaggug gucgacaagc agaccggcga gauccucagc     2340

guguccgaca acgaguacaa ggagcuggug uuccagccgc ccuaccaggg guucgucaac     2400

acgaucagcu ccaagggcuu cgaggacgag auccuguuca gcuaccaggu ggacuccaag     2460

uacaaccgca aggugagcga cgccaccauc uacuccaccc ggaaggcgaa gaucgggaag     2520

gacaagaagg aggagaccua cguccucggc aagaucaagg acaucuacag ccagaacggc     2580

uucgacacgu ucgugaagaa guacaacaag gacaagaccc aguuccugau guaccagaag     2640

gacccccuga ccugggagaa cgugaucgag gucauccucg gggacuaccc caccacgaag     2700

aaguccgagg acggcaagaa cgacgugaag ugcaaccccu ucgaggagua ccgccgggag     2760

aacgggcuga ucugcaagua cagcaagaag ggcaagggga cccccaucaa gucccugaag     2820

uacuacgaca agaagcucgg caacugcauc gacaucaccc ccgaggagag ccgcaacaag     2880

gugauccugc aguccaucaa cccguggcgg gccgacgucu acuucaaccc cgagacccug     2940

aaguacgagc ucaugggccu gaaguacagc gaccuguccu ucgagaaggg gacgggcaac     3000

uaccacauca gccaggagaa guacgacgcc aucaaggaga aggaggggau cggcaagaag     3060

uccgaguuca aguucacccu cuaccgcaac gaccugaucc ugaucaagga caucgccagc     3120

ggggagcagg agaucuaccg guuccucagc cgcaccaugc ccaacgugaa ccacuacgug     3180

gagcugaagc ccuacgacaa ggagaaguuc gacaacgucc aggagcuggu ggaggcccuc     3240

ggcgaggcgg acaagguggg ccggugcauc aaggggcuga acaagccgaa caucuccauc     3300

uacaaggucc gcaccgacgu gcugggcaac aaguacuucg ugaagaagga gggggacaag     3360

cccaagcucg acuucaagaa caacaagaag uga                                  3393


<210>  1666
<211>  3396
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1666
auggcccagc acguguucgg ccuggacauc gggaucgcca gcgucggcug ggcgauccuc       60

ggggagcagc gcaucaucga ccugggcgug cggugcuucg acaaggccga gaccgccaag      120

gagggggacc cccugaaccu cacccgccgg caggcccgcc ugcugcggcg ccggcucuac      180

cgccgggccu ggcgccugac gcagcugucc cggcuccuga agcgcaaggg ccugaucgcg      240

gacgccaagc uguucgccaa ggcccccagc uacggcgacu ccgcguggga gcugcggcgc      300

caggggcugg accggcuccu gacgccccug gagugggccc gcgugaucua ccaccagugc      360

aagcaccggg gcuuccacug gaccagcaag gccgaggagg ccaaggccga cuccgacgcg      420

gagggcgggc gcgucaagca ggggcucgcc cacaccaagg cccugaugca ggccaagaac      480

uaccggagcg ccgcggagau ggugcuggcc gaguuccccg acgcccagcg caacaagcgg      540

ggccaguacg acaaggcccu cucccgcgug cugcugggcg aggagcucgc ccugcuguuc      600

gcgacccagc gccggcucgg gaacccccac gccagcgacu ucuucgagaa gcugauccug      660

ggcgacgggg accgcaaguc cggccucuuc uggcagcaga agccggcccu gagcggcgcc      720

gaccugcuca agaugcuggg gaagugcacc uucgagaagg gcgaguaccg ggcccccaag      780

gcguccuuca gcgucgagcg ccacgugugg cugacccggc ucaacaaccu gcgcaucgug      840

gucgacgggc ggucccgccc ccugaacgag gccgagcggc aggccgcccu ccugcugccc      900

uaccagaccg agacgagcaa guacaagacc cucaagaacg cguucaucaa ggccggccug      960

uggggcgacg gggugcgcuu cggcgggcug gccuacccgu cccaggccca gaucgacgcc     1020

gagaagacca aggaccccga ggaccaguuc cucgugaagc ugcccgcgug gcacgagcug     1080

cggaaggccu ucaaggccgc cggccacgag gcccucuggc agcagaucag cacccccgcg     1140

cuggacgggg acccgacgcu gcuggaccag aucgccaccg uccucuccgu guacaaggac     1200

ggcgccgagg ugguccagca gcugcgccag cuggcccucc ccgagcccgc cgcgagcauc     1260

gccgugcugg agaagaucuc cuucgacaag uucagcagcc ugucccucaa ggcccugcgg     1320

cgcaucgugc cccugaugca gagcgggcuc cgguacgacg aggccgucgc gcagaucccg     1380

gaguacggcc accacuccca gcgcaucgag cccggcgccg ccaagcaccu guaccugccg     1440

cccuucuacg aggcccagcg gaaguacgcc gggaagggcg accacaucgg gagcaugcag     1500

uuccgcgacg acgcggacau cccccggaac cccguggugc uccgcgcccu gaaccaggcc     1560

cgcaaggucg ugaacgcccu gauccgggag uacggcuccc ccaucgccgu gaacaucgag     1620

auggcgcgcg accucagccg gccccuggac gagcgcaaca aggucaagcg ggcccaggag     1680

gaguuccgcg accggaacga ccgcgcccgg uccgaguucg agcgcgacuu cggguacaag     1740

ccgaaggccg ccgcguucga gaaguggaug cuguaccggg agcagcucgg ccagugcgcc     1800

uacagccagc agccccugga cauccagcgc gugcuggacg accacaacua cgcccagguc     1860

gaccacgccc uccccuacuc ccggagcuac gacgacucca agaacaacaa ggugcuggug     1920

cugacccacg agaaccagaa caagggcaac cgcaccgcgu ucgaguaccu cacgagcuuc     1980

cccgacgggg aggacggcga gcgguggcgc accuucgucg ccugggugca ggggaacaag     2040

gccuaccgga uggccaagcg caaccggcug cugcgcaaga acuacggcgu ggacgagucc     2100

aagggcuuca ucgaccggaa ccucaacgac acccgcuaca ucugcaaguu cuucaagaac     2160

uacgucgagg agcaccucca gcuggccgcg cgcgccgacg gggacaccgc ccggcgcugc     2220

guggugguca acggccagcu cacggccuuc cugcgggccc gcugggggcu gaccaaggug     2280

cggggcgaca gcgaccgcca ccacgcgcuc gacgccgccg uggucgccgc cugcacccac     2340

gggaugguga aggcgcuggc cgacuacucc cggcgcaagg agaucagcuu ccugcaggag     2400

ggcuucccgg accccgagac cggcgagauc cucaaccccg ccgccuucga ccgggcccgc     2460

cagcacuucc cggagcccug gacgcacuuc gcgcacgagc ugaaggcccg gcuguucacc     2520

gacgaccucg ccgcccugcg cgaggacaug cagcggcugg gguccuacac caccgaggac     2580

cucggccgcc ugcggacgcu guucgugagc cgcgcgcccc agcggcgcuc cgggggcgcc     2640

guccacaagg agaccaucua cgcccagccc gagagccuca agcagcaggg cggggugauc     2700

gagaagaucc ugcugaccag ccucaagcug caggacuucg acaagcugcu caacccggag     2760

uccaacgacc acuucgugga gccccaccgg aacgagcgcc uguacgccgc cauccgccag     2820

cggcuggagc aguucggcgg gcgcgcggac aaggccuucg gccccgacaa ccucuuccac     2880

aagcccgaca agaacaacca gccgaccggg cccgucgugc ggagcaucaa gcuggugcgc     2940

ggcaagcaga cggggauccc cauccggggc ggccuggcca agaacgacuc caugcuccgc     3000

gucgacaucu ucaccaaggc cgggaaguuc caccuggugc ccguguacgu ccaccaccgg     3060

gugaccggcc ugccgaaccg cgccaucgug gcguucaagg acgaggacga guggacccuc     3120

aucgacgaga gcuucgccuu ccuguucucc gucuacccca acgacuacgu gaaggugacg     3180

cugaagaagg agcagcagag cggguacuac uccggcgccg accggagcac cggggccaug     3240

aaccucuggg cccacgaccg cgcggccucc gucggcaagg acggccugau ccgggggauc     3300

ggcgugaaga ccgcccugag cguggagaag uucaacgucg acgugcucgg gcgcaucuac     3360

cuggccccgc ccgagacccg guccggccug gcguga                               3396


<210>  1667
<211>  3399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1667
auggcccagu acguguucgg ccuggacauc gggaucgcca gcgucggcug ggcggugcuc       60

gacgcccagc gcaucgugga ccuggggguc cggugcuucg acaaggccga gaccgccaag      120

gagggcgacc cccugaaccu cacccgccgg caggcccgcc ugcugcggcg ccggcucuac      180

cgccgggcgu ggcgccugac gcagcuggcc cggcuguuca agcgcgaggg gcugaucgac      240

gacgccaagc uguucgccaa ggcgccgucc uggaccgaga acgccuggga gcuccggcug      300

cgcggccugg accaccuccu gacgccccug gagugggccc gggugaucua ccaccucugc      360

aagcaccgcg gcuuccacug gaccagcaag gccgaggagg ccaaggcgga guccgacgcc      420

gagggcgggc gggugaagca ggggcuggcc cacaccaagg cccugaugca ggucaagaac      480

uaccgcagca ccgccgagau ggugcucgcg gaguuccccg gcgcccagcg gaacaagcgc      540

gggcaguacg acaaggcccu gucccgggug cugcucggcg aggagcuggc ccuccucuuc      600

gccacccagc gccgccuggg caacccccac gcgagcgagc acuucgagca gcugauccuc      660

gggcacggcg accggaaguc cgggcuguuc uggcagcaga agccggcccu gagcggcgcc      720

gaccuccuga agaugcuggg caagugcacc uucgagaaga cggaguaccg cgcccccaag      780

gccuccuuca gcgcggagcg gcacgucugg cucacccgcc ugaacaaccu gcggaucgug      840

guggacgggc gcgcccggcc ccucaacgac gccgagcgcc aggccgcgcu gcugcucccc      900

uaccagaccg agaccuucaa guacaagacg cugaaggccg ccuucgucaa ggugggccug      960

uggggcgacg gggugcgguu cgggggccuc gccuacccgu cccaggccca ggcggaggcc     1020

gagaaggcca aggaccccga ggaccaguuc cuggucaagc ugcccgccug gcacgagcuc     1080

cgcaaggccu ucaaggcggc cggccacgag gcccuguggc agcagaucag caugcccgcc     1140

cuggaggagg acccgacccu gcucgaccag aucgccaccg ugcuguccgu guacaaggac     1200

gggaccgagg ucgugcagca gcugcggcag cucgcgcugc ccgagcccga cgccagcauc     1260

gccgugcugg agaagauccg cuucgacaag uucuccagcc ucucccugaa ggcccugcgg     1320

cgcaucgucc cccucaugca gggcgggcug cgguacgacg aggcgguggc ccagaucccg     1380

gaguacggcc accacagcca gcgcaucgac cccggggcca cgcggcaccu guaccucccg     1440

cccuucuacg aggcccagcg caaguacgcc ggcaagggcg accggaucgg guccaugcag     1500

uuccgcgagg acgcggacgu gccccgcaac cccgucgugc ugcgggcccu gaaccaggcc     1560

cgcaaggucg ugaacgcccu cauccgggag uacggcagcc ccguggccgu caacaucgag     1620

auggcgcgcg accugagccg gccccuggac gagcgcaaca aggugaagcg ggcccaggag     1680

gaguuccgcg accggaacga caaggcccgc uccgaguucg agcgggacuu cggguacaag     1740

ccgaagggcg ccguguucga gaaguggaug cucuaccgcg agcagcuggg gcagugcgcc     1800

uacagccagc agccccugga cauccagcgg guccucgacg accccaacua cgcccaggug     1860

gaccacgccc ugcccuacuc ccgcagcuac gacgacucca agaacaacaa ggugcugguc     1920

cucacccggg agaaccagaa caagggcaac cgcaccccgu ucgaguaccu gaccagcuuc     1980

cccggcgggg aggacggcga gcgguggcgc gccuucgugg ccugggugca ggggaacaag     2040

gcguaccgga uggccaagcg caaccggcug cuccgcaaga acuacggcac ggacgagucc     2100

cgcggcuuca ucgaccggaa ccugaacgac acccgcuaca ucugcaaguu cuucaagaac     2160

uacgucgagg agcaccucca gcuggccgcc cggaccgacg gggacaccgc ccgccggugc     2220

guggugguca acggccagcu gacggcguuc cugcgcgccc ggugggggcu cgccaaggug     2280

cgcggcgaca gcgaccggca ccacgcccug gacgccgcgg uggucgccgc cugcacccac     2340

gggaugguga aggcccuggc cgacuacucc cgccggaagg agaucgcguu ccuccaggag     2400

ggcuuccccg acccggagac cggcgagauc cugaaccugg ccgccuucga ccgcgcccgg     2460

gagcacuucc ccgagcccug gccccacuuc gcccacgagc ucaaggcgcg ccuguucauc     2520

gacgaccugg uggcccuccg ggaggacaug cagcaccugg ggagcuacgc ccaggacgac     2580

cuggaccgcc uccggacccu guucgucucc cgcgccccgc agcggcgcaa cggcggggcg     2640

gugcacaagg gcacgaucua cgcccagccc gagaaccuga agcagcaggg gagcaccauc     2700

gagaagaucc uccugaccgg ccugaagcuc caggacuucg acaagcugcu gaaccccgag     2760

uccagcgacc acuucgugga gccccaccgc aacgagcggc ucuacgccgc cauccgccgg     2820

cgccuggagg aguucggcgg gaaggccgac aaggcguucg gcccggacaa gcccuucuac     2880

aagcccgaca agaacgggca gcccaccggc ccgaucgucc gguccaucaa gcuggugcgc     2940

gggaagcaga cgggcauccc cauccggggc gggcucgcca agaacgacag caugcugcgc     3000

guggacgucu ucaccaaggc cggcaaguuc caccuggugc ccguguacgu ccaccaccgg     3060

gugaccgggc uccccgaccg cgccaucgug gccuucaagg acgaggagga guggacccug     3120

aucgacgagu ccuacgcguu ccuguucagc gucuacccga acgacuucgu gugcgugcag     3180

cagcggggca agccccucau cgagggguac uacgccggcc uggaccgcgc caacggcaac     3240

aucaacgucu gggcccacga ccgggccgcg uccgugggga aggacggccu gauccgcggg     3300

aucggcguga agacggcccu cagccuggag aagcugaacg ucgacgugcu cggccggauc     3360

uaccccgcgc ccccggagac ccgccggggg cuggccuga                            3399


<210>  1668
<211>  3399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1668
augaagaaga uccugggcgu ggaccucggg aucaccagcu ucggcuacgc cauccugcag       60

gagaccggga aggaccugua ccgcugccuc gacaacuccg ucgugaugcg gaacaacccc      120

uacgacgaga aguccggcga guccagccag uccauccgca gcacgcagaa guccaugcgg      180

cgccugaucg agaagcggaa gaagcgcauc cggugcgugg cccagaccau ggagcgcuac      240

gggauccugg acuacagcga gaccaugaag aucaacgacc cgaagaacaa ccccaucaag      300

aaccgguggc agcuccgcgc ggucgacgcc uggaagcggc cccugagccc ccaggagcug      360

uucgccaucu ucgcccacau ggccaagcac cgcggcuaca agagcaucgc gaccgaggac      420

cucaucuacg agcuggagcu ggagcucggc cugaacgacc cggagaagga guccgagaag      480

aaggccgacg agcggcgcca gguguacaac gcccugcggc accucgagga gcugcgcaag      540

aaguacgggg gcgagacgau cgcccagacc auccaccggg cgguggaggc cggggaccug      600

cgcagcuacc ggaaccacga cgacuacgag aagaugaucc gccgggagga caucgaggag      660

gagaucgaga agguccuccu gcgccaggcc gagcugggcg cccucgggcu gcccgaggag      720

caggucuccg agcugaucga cgagcucaag gccugcauca ccgaccagga gaugcccacc      780

aucgacgaga gccuguucgg caagugcacg uucuacaagg acgagcuggc ggcccccgcc      840

uacuccuacc ucuacgaccu guaccgccug uacaagaagc ucgccgaccu gaacaucgac      900

ggcuacgagg ugacccagga ggaccgggag aaggugaucg agugggugga gaagaagauc      960

gcccagggga agaaccugaa gaagaucacc cacaaggacc uccgcaagau ccugggccug     1020

gcgccggagc agaagaucuu cgggguggag gacgagcgga ucgucaaggg caagaaggag     1080

ccccgcaccu ucgugcccuu cuucuuccuc gccgacaucg ccaaguucaa ggagcuguuc     1140

gccagcaucc agaagcaccc cgacgcccug cagaucuucc gggagcucgc ggagauccug     1200

cagcgcucca agacgccgca ggaggcccug gaccggcucc gcgcccugau ggccggcaag     1260

gggaucgaca ccgacgaccg ggagcugcuc gagcuguuca agaacaagcg cagcggcacc     1320

cgggagcugu cccaccgcua cauccucgag gcccugcccc uguuccugga gggguacgac     1380

gagaaggagg ugcagcggau ccucggcuuc gacgaccgcg aggacuacag ccgguacccc     1440

aagagccugc gccaccucca ccuccgggag gggaaccugu ucgagaagga ggagaacccc     1500

aucaacaacc acgcggucaa gucccuggcc agcugggccc ugggccugau cgccgaccug     1560

uccuggcgcu acggcccguu cgacgagauc auccucgaga ccacgcggga cgcgcugccc     1620

gagaagaucc gcaaggagau cgacaaggcc augcgcgagc gggagaaggc ccuggacaag     1680

aucaucggga aguacaagaa ggaguucccc agcaucgaca agcgccucgc ccggaagauc     1740

cagcuguggg agcgccagaa gggccuggac cucuacuccg ggaaggugau caaccugagc     1800

cagcugcucg acggcuccgc cgacaucgag cacaucgugc cccagagccu gggcgggcug     1860

uccaccgacu acaacaccau cgucacccuc aagagcguga acgcggccaa gggcaaccgg     1920

cugccggggg acuggcuggc cggcaacccc gacuaccgcg agcggaucgg gaugcucucc     1980

gagaagggcc ugaucgacug gaagaagcgc aagaaccugc ucgcccagag ccuggacgag     2040

aucuacacgg agaacaccca cuccaagggg auccgggcca ccagcuaccu ggaggcgcuc     2100

guggcccagg uccugaagcg cuacuacccc uuccccgacc cggagcugcg gaagaacggc     2160

aucggcgugc gcaugauccc cgggaaggug accuccaaga cgcggagccu ccugggcauc     2220

aaguccaaga gccgcgagac caacuuccac cacgccgagg acgcccugau ccucuccacc     2280

cugacccggg gguggcagaa ccgccugcac cggaugcucc gcgacaacua cggcaagagc     2340

gaggccgagc ugaaggagcu guggaagaag uacaugcccc acaucgaggg ccucacgcug     2400

gcggacuaca ucgacgaggc cuuccggcgc uucaugagca agggggagga gucccuguuc     2460

uaccgggaca uguucgacac cauccgcagc aucuccuacu gggucgacaa gaagccccuc     2520

agcgccucca gccacaagga gaccguguac uccagccgcc acgagguccc gacccugcgg     2580

aagaacaucc uggaggccuu cgacucccuc aacgugauca aggaccgcca caagcugacg     2640

accgaggagu ucaugaagcg guacgacaag gagauccgcc agaagcugug gcuccaccgg     2700

aucggcaaca ccaacgacga gagcuaccgc gcgguggagg agcgggccac ccagaucgcc     2760

cagauccuga cgcgcuacca gcugauggac gcccagaacg acaaggagau cgacgagaag     2820

uuccagcagg cccucaagga gcugaucacc ucccccaucg aggucaccgg gaagcugcuc     2880

cggaagaugc gcuucgugua cgacaagcug aacgcgaugc agaucgaccg gggccuggug     2940

gagaccgaca agaacaugcu cgggauccac aucagcaagg gccccaacga gaagcugauc     3000

uuccgccgga uggacgucaa caacgcccac gagcugcaga aggagcgcuc cggcauccuc     3060

ugcuaccuga acgagaugcu guucaucuuc aacaagaagg ggcucaucca cuacggcugc     3120

cugcggagcu accuggagaa ggggcagggc uccaaguaca ucgcccucuu caacccgcgc     3180

uuccccgcca accccaaggc ccagcccagc aaguucaccu ccgacagcaa gaucaagcag     3240

gucgggaucg gcagcgcgac gggcaucauc aaggcccacc uggaccugga cgggcacgug     3300

cgguccuacg agguguucgg cacccucccg gaggggagca ucgagugguu caaggaggag     3360

uccggcuacg ggcgcgugga ggacgacccc caccacuga                            3399


<210>  1669
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1669
auggaccuga acuaccgcau cggccucgac aucgggaucg ccagcguggg cugggccguc       60

cuggagaaca acuccaagga cgagcccgug cggaucgugg accugggggu ccgccucuuc      120

gaggcggccg aggugccgaa gaccggcgcc gcccuggccg agccccggcg cgaggcgcgg      180

accacgcgcc ggcgccugcg gcgccggaag caccgccucg agcggaugaa guggcugcug      240

caggaguacg ggcucaucca gaccgaggag uucaugaagc gcuaccacag cgccggccug      300

cccgacgugu accggcugcg cuacgagggc cucgagcgga agcugaagga cgaggagcug      360

gcccagaucc ucauccacau cgggaagcac cgcggcuucc gguccacccg caaggccgag      420

cugaaggaca agaacgacaa ggagaccggg gcgguccuga gcgccacgcg ggagaacgag      480

aagcucaugg aggagcacgg cuaccgcacc gccggggaga ugaucuacaa ggacgacaag      540

uuccgcaccg gcugcaccug gaaggaggag ggcuaccggc ugacgccccg caacaaggag      600

ggggacuaca agaacaccau gcugcgggcc cuccuggagg acgaggugaa ggugaucuuc      660

gacugccagc gcaaguucgg caaccagaag gccuccgagg agcuggagaa ggcguaccuc      720

gccaucauga ccagccagcg guccuucgac auggggccgg gcaagcagcc cgacggcagc      780

gccucccccu acgccaugga gggguucggc gaccgcgucg ggcugugcgc ccuggagccc      840

cgggagaagc aggagccgcg cgcgcccaag gccgccuaca ccagcgagcu guucguggcc      900

cugcagaaga ucaaccaccu ccggaucacg gagaagaacg gcaaggggcg cggccucacc      960

uccgaggagc gggagcgccu gagcgaccug cuccacaaga agaaggagcu gaccuacggc     1020

gccgugcgga aggagcugca gcucgaccug cgcuaccggu ucaacacccu gaacuacucc     1080

agcaagaaga ugacggagga ggagaagauc aaggacgcgg agaaggccaa guucgucucc     1140

auggugagcc acuacgagua ccgcaagcgg auguccggga ugcucgagca gcugagcccc     1200

gaggagcagg ccgaccugau ggacgaggug ggcaagaucc ucacccugua caagaacgac     1260

gacacccgcu cccgggaguu ccagaagcug gggcucgaca gcgagaucac cgaggagcug     1320

cuggagcuca acccccgcgg cuuccagcac gucagccuga aggccaugaa gaagcugaug     1380

ccguaccuga aggaggggcu cacguacgac aaggccugcg cggaggccgg cuacgacuuc     1440

cagggcuccg ugcagaagga gcgcagccgg cugcugaagg ggaaggagau caccgacauc     1500

aucaacgaca ucaacaaccc cguggucaag cgcuccguga gccagaccgu gaaggucauc     1560

aacgccauca uccagaagua cggcagcccc caggccguga acaucgagcu cgcgcgggag     1620

augucccgca acuacgacga gcggcgcaag auggagaagc agaugaagga ccggcagucc     1680

gagaacgagc gcgccaagaa ggagauccag gagcugggga acgccuuccc caccggccag     1740

gacauccuga aguaccggcu cuggcacgac caggacggga ucugcaugua cagcgccaag     1800

cgcaucccgc uggccgagcu guucacgggc gcguacgaca ucgaccacau ccuccccuac     1860

uccaucaccu ucgacgacag cuaccggaac aaggugcugg ucaccuccga ggagaaccgc     1920

cagaagggca accggauccc cuacgaguac uucgggagcg acuccgagcg cuggagcaag     1980

uucgagaccc uggugaacuc ccggauccgc gacuaccgga agcagcagaa ccuccugaag     2040

cagagcuuca cggaggagga gcgcaaggag uucaagcagc ggaaccugaa cgacaccaag     2100

uacaucaccc gcgucaucua caacaugauc cgggagaacc ucgcccuggc ccccuacaac     2160

uccccgggca agaagaagca ggucuucgcc gugaacgggg gcgugaccgc cuaccugcgc     2220

aagcgcuggg gcaugagcgc gaaggaccgg uccaucgaca cgcaccacgc ccgcgacgcc     2280

guggugaucg ccugcugcac cgacgggaug auccagaaga ucagccggaa cauccagggc     2340

cgcgagcucc uguacgcccg gggguucacc gcggucgacg uggagaccgg cgagaaguuc     2400

acgcgcgacg acuucacccg ggaccagugg gacgagaucu ucggggugca gguccccaag     2460

cccugggagu acuucaacga ggagcuggac auccgccucg gcgagaaccc cguggaguuc     2520

aucgagaccc acagcgacgu gucccggaag cuggacuacc cggccugguu cuacgagaac     2580

aacaucaucc gccccgucuu cgugagccgg augcccaacc gcaaggugac cggcgccgcc     2640

cacgcggaca ccauccggag cccccgccac uaccaggaga cgggguccgu ccugaccaag     2700

accgcccuca ucgaccugaa gcugaacaag aagaccggcg agaucgagaa cuacuacaag     2760

ccggaguccg acacgcuccu guacaacgcc cugaaggagc ggcucgagcu guacgggggc     2820

gacgggaaga aggccuucga ggagcccuuc uacaagccca aggccgacgg cagccgcggc     2880

aaccuggugc ggaaggugaa gaccuacgac aagcucaccc ugggggucua cgugaacucc     2940

cagcacggca ucgcggccaa cgccaacggg agcauggugc gcgucgacgu guuccgggug     3000

uccggcaagu acuacuucgu cccgguguac aucgccgacg ugguccgcaa gcgccugccc     3060

augaaggccg uggugcaggg gaagcaguac aucgacugga aggagaugga ggacaaggac     3120

uucaucuuca gccucuacac ccgggaccug aucuccuuca agagcaagaa cggcaagaag     3180

gucacgugcg uggacggcac cuccaaggcg gugaacgagg agaucgucua cuacuacggg     3240

gccaacauca gcaccgccuc cuucagcggc aagucccaca acgggagcuu cgaguucacc     3300

agccugggca uccaggggcu cgaguaccug aagaaguacc agguggacgu gcugggcaac     3360

gucuccgagg ugaagcgcga gaagcggaug ggcuuccgcu ga                        3402


<210>  1670
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1670
augggcgggu ucgaggugga ccgcaagauc agccugggcc ucgacaucgg ggucuccagc       60

gugggcuacu ccgugaucga cgucgacacc gggaaggugc uggagcuggg cagccggcuc      120

aucaacggca agaucgggac cgagaaccag gcccgccggg agaugcgcgg cacgcggcgc      180

cugaucaacc ggaagaagca gcgccggaag gacaccguga agcuguucaa cgaguucaag      240

cucaucgacu ccaagaagga cgagcacuac uucgaccacu ucgccaacaa cagcgacccc      300

uacgagcugc gcgucaaggg gcuguccgag aagcucagcc uguccgagau cgcggacggc      360

cuguaccaca ucgugaagcg gcgcgggauc agcuacgacc ucaaggacgc cgacgaggag      420

uccgagggca gcgaguacga gaacggccug aagaucaaca acuccgagcu gaagaaccag      480

accccggccc agauccagcu caagcggcug aacgaguacg gggccauccg cggcaaggug      540

gucaucgacg aggagaagau ccugcucaac guguucccca ccaaggccua ccuggacgag      600

gcgaagcgga ucaucgagaa gcagcgcgag uucuaccccg ggauccugac ggaggaguuc      660

gaggagaagu acugccagau ccucacccgg aagcgcgacu acuucguggg ccccggcagc      720

gagaaguccc ggaccgacua cgggaucuac aaggaggacg gccgcacccu gaacaaccug      780

uucgaggagc ucaucgggca cgacaagauc uacccggagg agcugcgggc cagcggcgcc      840

uccuucacgg cccagcuguu caacguccuc aacgaccuga acaaccugca caucagcucc      900

uacgaggacc agaagcucac caccgagaac aaggagcaga ucaucagcga gcucaagagc      960

accacgucca acgugaacau gaugaagcug auccagaaga ucgcggggug cgaggagagc     1020

gacaucuccg gcuaccgcac cgacaagaac uacaagcccg agaucagcuc caugcgcauc     1080

uaccggaagg ugcacaagaa gcuccuggag agcgagaucg acaucaccaa cuggcccgac     1140

gacuucauca acaagcuguc cuucauccuc acccugaaca cggagaacgg cgagauccgc     1200

aaccagcuga agaaccggcu ccagcccgag uacgacuucc ugaccgacga gcugauccag     1260

accaucaucg acaacaagga gagcuucgac aucaagucca acaacaagug gcaccgcuuc     1320

agcaucaaga ccauggagcu ccugaucccg gagaugauca agcggcccgu cgagcagaug     1380

acgcugauca acgagauggg gcucaucaag aacuccggca agaaguucag caacuacaag     1440

uaccugcccu acaagaagau cgccgaggac aucuacaacc ccguggccuc caagagcgug     1500

cgcgaggccc ugaagaucgu caacgccauc cucaaggagu acgggcacau cgaguacauc     1560

gugguggaga ugccgcggga caagaacgag gacgaggcga aggaccagau cgagaaguuc     1620

cagaaggcca acggccgccu gaaggacgcc gcccuggagg aguucaccga ggccaucggg     1680

gacaagucca ccgucgacaa gaagcugggc gugcaccgga gcaagcuccu guucaagauc     1740

cgccuguggu accagcagcg gggcauggac cucuacgagg ggaagcccau cuccgcggug     1800

gaccugcuga acaaccccga cagcuuccag aucgaccaca ucauccccca guccgucagc     1860

uucgacgaca gcuucaacaa caagacccuc ugcaacuccg gccugaacca gaucaaggac     1920

aagaagacgc cguucgagua caugaacgag ggguacggcc agggguucga cagcaugaag     1980

gccaugguga ugaagaaccc ccgcaucgac cacaacaagc ggaagaacua ccuguucucc     2040

gagaacguca gcgacaucga gacccgcaag cgguucaucu cccgcaaccu cguggacacc     2100

cgguacgcca gccgcguggu ccugaacaac cugcaggagu ucuucaagga gaaggagucc     2160

gacaccaaga ucagcaucau ccggggcaag uucacgucca accuccgcaa gcacuggaag     2220

aucaacaaga accgcgacac cuaccaccac cacgccaucg acgccaccau caucgcggcc     2280

acccccuucc ugaacaucug gaagaagggc agcuccaucu uccccagcaa cguggagucc     2340

gaggacuucu ucgacaucaa gacggggaag aucaucggca acgacgaguu caacaaguac     2400

gccuacgacg agccguacaa cggguucuac aacgagcugc ggaacgccac cgaccgcauc     2460

aaguucagcc accagaucga caagaagaug aaccggaagg uguccgacag caccaucuac     2520

uccacccgcg ucgccaagag ccccaagaac aagaaggaga gcgaguacau cguggcgaag     2580

aucaagaaca ucuacgacgc cgacgaguac gccaaguuca agcggaucua cgacaaggac     2640

aagacgaagu uccuccugaa caaguacgac ccccgcuccu ucaaccaccu ggagaagauc     2700

augaacaccu accccgacag caucgagcgg accaugucca acaacaaggu gaaggucgug     2760

cgcaucagcc cguucgagcu cuaccggcag gaccacggcc ccgugaagaa guacuccaag     2820

aagaacaacg gccccgagau caagcagcug aaguaccugg acaagaaggu cgggagcucc     2880

aucgacguga cccccgacaa cgccaagaac aagcacguga uccucaagag ccugaagccg     2940

uggcgcaccg acaucuaccu gaaccacgag acgggcgagu acgagaucau ggggaucaag     3000

uacuccgacc ucaaguucaa caagggcgag ggguacggca ucaagaaggc gaaguaccug     3060

gagaucaagc accgggagaa ggucagcgac gacuccgagu ucauguucac ccuguacaag     3120

aagaaccgca ucaaggugga gaacauggag accgacgaga gcguggagau gcuguucugg     3180

ucccggacca uggacagcca gaagggcuac gccgagcuga agcccaucua caaguacaag     3240

acggaggacg acaacuggcc caucuacggg aacgccaaga agcagaucau caagaagcug     3300

aucccgaaga acugcaagau cuggaagguc aacaccgacg ugcucggcaa ccccuucuac     3360

guggagaagg agggggagua ccccaaggac guccuggacu ga                        3402


<210>  1671
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1671
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaagaacu ccgggaagau cgugcacgcc agcucccgca ucuuccccgc ggccaccgcc      120

gacaacaacg ucgagcggcg caagaaccgg caggcccgcc ggcuccaccg ccggaagaag      180

caccgcggcg cccggcugaa ggaccuguuc gaguacuacg gccuccugac cgacuucagc      240

aaggugucca ucaaccugaa cccguaccgc cuccgggugg acgggcugga ccagcagcug      300

acgaacgagg agcuguucau cgcgcucaag aacaucguca agcgccgggg caucagcuac      360

cuggacgacg ccuccgagga cgggggcacc gugagcuccg acuacgggaa ggccguggag      420

gagaaccgca agcuccuggc cgagcagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc agcuccgcgg ggacuucacc gucguggaga acagcgagaa gugccggcug      540

aucaacgugu ucuccacgag cgcguacaag aaggaggccg agcgcauccu gcggaagcag      600

caggaguuca acaaccagau caccgacgag uucaucgagg acuaccucaa gauccugacc      660

ggcaagcgca aguacuacca cgggcccggc aacgagaagu cccggaccga cuacggccgc      720

uuccgcacgg acggggccac ccuggacaac aucuucggca uccucaucgg gaagugcacc      780

uucuacccca acgaguaccg ggccagcaag gccuccuaca ccgcgcagga guucaaccug      840

cugaacgacc ucaacaaccu gacggucccg accgagacca agaagcugag cgaggagcag      900

aagaagacca ucaucgagua cgccaagucc gccaagacgc ucggcgccag cacccugcug      960

aaguacaucg ccaagaugau cgacgcgucc guggaccaga uccgcgggua ccggguggac     1020

gucaacaaca agcccgagau gcacaccuuc gagguguacc gcaagaugca gagccucgag     1080

accaucagcg ugggcgagcu gucccggaac auccuggacg agcucgccca cauccugacg     1140

cugaacaccg agcgcgaggg caucgaggag gccaucaaca ccaagcuccg ggacagcuuc     1200

ucccaggacc agguccugga gcuggugcag uuccgcaaga acaacagcuc ccucuucagc     1260

aagggguggc acaacuucuc ccugaagcug augauggagc ucauccccga gcuguacgag     1320

accagcgagg agcagaugac gauccugacc cggcucggca agcagaaguc caaggagacc     1380

agcaagcgca ccaaguacau cgacgagaag gaggugacgg aggagaucua caaccccguc     1440

guggccaagu ccgugcggca ggccaucaag aucaucaacg aggcgaccaa gaagcacggg     1500

aucuucgaca acaucgucau cgagauggcc cgcgagaaca acgaggagga cgccaagaag     1560

gacuacauca agcggcagaa ggccaaccag gacgagaagu acgccgcgau ggagaaggcc     1620

gccuuccagu acaacggcaa gaaggagcug ccggacaaca ucuuccacgg gcacaaggag     1680

cuggccacca agauccgccu guggcaccag cagggcgaga agugccucua caccggcaag     1740

agcaucccca ucuccgaccu gauccacaac caguacaagu acgagaucga ccacauccug     1800

ccccucagcc uguccuucga cgacagccug uccaacaagg ugcucguccu ggcgacggcc     1860

aaccaggaga aggggcagcg gacccccuuc caggcccugg acagcaugga cgacgccugg     1920

uccuaccgcg aguucaagag cuacgugaag gagagcaagc uccugggcaa caagaagaag     1980

gaguaccugc ucaccgagga ggacaucucc aagaucgagg ugaagcagaa guucaucgag     2040

cggaaccugg ucgacacccg cuacagcucc cggguggugc ugaacgcccu ccaggacuuc     2100

uacaagaacc acaacuucga cacgaccauc agcgucgugc gcgggcaguu caccucccag     2160

cugcgccgga aguggggccu ggagaagucc cgcgagaccu accaccacca cgcgguggac     2220

gcccucauca ucgccgccag cagccagcug cggcugugga agaagcagaa caacccgcuc     2280

aucgccuaca aggaggggca guucgucgac ucccagaccg gcgagaucau cagccugacg     2340

gacgacgagu acaaggagcu gguguucaag gcgcccuacg accacuucgu ggacacccuc     2400

uccagcaaga ccuucgagga cuccauccug uucagcuacc aggucgacuc caaguucaac     2460

cgcaagauca gcgacgccac caucuacgcc acgcggaagg ccaagcugga caaggagaag     2520

aaggaguaca ccuacacccu cggcaagauc aaggacaucu acucccuggg gaccaagacg     2580

cccagcaaga ccggcuucua caaguuccug gaccucuaca acaaggacaa gagccaguuc     2640

cugauguucc agaaggaccg caagaccugg gacgagguga ucgagaagau cauggagcag     2700

uaccggcccu ucaaggagua cgacaaggcc gggaagcugg uggacuucaa cccguucgag     2760

aaguaccgcc aggagaacgg ccccauccgg aaguacucca agaagggcaa cgggcccgag     2820

aucaagagcc ucaaguacua cgacauccug cugggcaagc acaagaacau cacccccgag     2880

gggucccgca acacggucgc gcuccugagc cugaacccgu ggcggaccga cguguacuac     2940

aacauggaga ccaagaagua cgaguuccuc ggccugaagu acgccgaccu gcccuucgag     3000

gagggcgggg ccuacggcau cuccaccgag acguacaacg agcuccgcga gaaggagggg     3060

aucggcaaga acagcgaguu caaguucacc cuguacaaga acgaccugau ccucaucaag     3120

gacaccgaga ccaacugcca gcaguucuuc cgguucuggu cccgcacggg gaaggacaac     3180

cccaagagcu ucgagaagca caagaucgag cugaagcccu acgagaaggc caaguucgag     3240

aagggcgagg agcuggaggu gcucgggaag gucccgcccu ccagcaacca guuccagaag     3300

aacaugcaga ucgagaaccu guccaucuac aaggugaaga ccgacgugcu gggcaacaag     3360

cacuucauca agaaggaggg cgacaagccc aagcucaagu ucuga                     3405


<210>  1672
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1672
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaagaacu ccgggaagau cauccacgcc aacagccgcc ucuuccccgc ggccaccgcc      120

gacaacaacg uggagcggcg caagaaccgg caggcccgcc ggcugaaccg ccggaagaag      180

caccgcggcg uccgguucca ggaccuguuc gaggacuacg gccuccugac cgacuucucc      240

aaggugagca ucaaccugaa cccguaccgc cuccgggugg acgggcugaa ccagcagcug      300

acgaacgagg agcuguucau cgcccucaag aacaucguca agcgccgggg caucuccuac      360

cuggacgacg cgagcgagga cgggggcgcc guguccagcg acuacgggaa ggccguggag      420

gagaaccgca agcuccuggc cgagcagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc agguccgcgg ggacuucacc gugguggaga acggcgagaa gcuccggcug      540

aucaacgucu ucuccaccag cgcguacaag aaggaggccg agcgcauccu gcggaagcag      600

caggaguuca acaaccagau cacggacgag uucaucgagg acuaccucaa gauccugacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccggaccga cuacgggcgc      720

uuccgcaccg acggcacgac ccuggacaac aucuucggga uccucaucgg caagugcacc      780

uucuaccccg aggaguaccg ggccagcaag gccuccuaca ccgcccagga guucaaccug      840

cugaacgacc ucaacaaccu gacggugccg accgagacca agaagcugag cgaggagcag      900

aagaagguga ucgucgagua cgcgaaggag gccaagaccc ucggggccuc cacgcugcug      960

aaguacaucg ccaagaugau cgacgccagc guggaccaga uccgcggcua ccggguggac     1020

gucaacaaca agcccgagau gcacaccuuc gagguguacc gcaagaugca gucccucgag     1080

accaucagcg ugggcgagcu gucccggaac guccuggacg agcucgcgca cauccugacc     1140

cugaacacgg agcgcgaggg gauggaggag gccaucaaca ccaagcucaa ggacagcuuc     1200

agccaggacc aggugcugga gcuggugcag uuccggaaga acaacuccag ccucuucucc     1260

aagggcuggc acaacuucag ccugaagcug augauggagc ucauccccga gcuguacgag     1320

accuccgagg agcagaugac cauccugacg cgccucggga agcagaaguc caaggagacc     1380

agcaagcgga ccaaguacau cgacgagaag gagcugaccg aggagaucua caaccccguc     1440

guggccaaga gcguccgcca ggccaucaag aucaucaacg aggccacgaa gaagcacggc     1500

aucuucgaca acaucgugau cgagauggcg cgggagaaca acgaggagga cgccaagaag     1560

gacuacauca agcgccagaa ggccaaccag gacgagaaga acgccgccau ggagaaggcg     1620

gccuuccagu acaacgggaa gaaggagcug ccggacaaca ucuuccacgg ccacaaggag     1680

cuggccacca agauccggcu cuggcaccag cagggcgaga agugccugua caccgggaag     1740

accaucccca ucuccgaccu gauccacaac caguacaagu acgagaucga ccacauccuc     1800

ccccugagcc uguccuucga cgacagccuc uccaacaagg ugcugguccu ggccacggcg     1860

aaccaggaga agggccagcg cacccccuuc caggcccucg acagcaugga cgacgccugg     1920

uccuaccggg aguucaagag cuacgugaag gaguccaagc ugcuggggaa caagaagaag     1980

gaguaccucc ugaccgagga ggacaucagc aagaucgagg ugaagcagaa guucaucgag     2040

cgcaaccuga ucgacacccg guacuccagc cgcgucgugc ucaacgcccu gcaggacuuc     2100

uacaaggccc acaaguucga cacgaccauc agcguggucc ggggccaguu caccucccag     2160

cugcgccgca agugggggcu cgagaagucc cgggagaccu accaccacca cgcgguggac     2220

gcccugauca ucgccgccag cagccagcug cgccucugga agaagcagaa caacucccug     2280

aucagcuaca aggagggcca guucguggac uccgagaccg gcgagaucau cagccugacg     2340

gacgacgagu acaaggagcu cgucuucaag gccccguacg accacuucgu ggacacccug     2400

cgguccaaga aguucgagga cagcauccug uucuccuacc agguggacag caaguucaac     2460

cgcaagaucu ccgacgcgac caucuacgcc acccggaagg ccaagcucga caaggagaag     2520

aaggaguaca cguacacccu ggggaagauc aaggacaucu acagccuggg caccaaggcc     2580

cccuccaaga ccggguucua caaguuccuc gaccuguaca acaaggacaa gagccaguuc     2640

cugauguucc agaaggaccg caagacgugg gacgagguca ucgagaagau cauggagcag     2700

uaccggcccu ucaaggagua cgacaagacc ggcaagcucg uggacuucaa ccccuucgag     2760

aaguaccgcc aggagaacgg cccgauccgg aaguacucca agaaggggaa cggccccgag     2820

aucaagagcc ugaaguacua cgacauccug cucgggaagc acaagaacau cacccccgag     2880

ggcagccgca acaccguggc ccugcugucc cucaaccccu ggcggacgga cgucuacuac     2940

aacauggaga ccaagaagua cgaguuccug gggcugaagu acgcggaccu cccguucgag     3000

gagggcggcg ccuacgggau cagcaccgag accuacaacg agcugcgcga gaaggagggc     3060

aucgggaaga acuccgaguu caaguucacg cuguacaaga acgaccucau ccugaucaag     3120

gacaccgaga ccaacugcca gcagaucuuc cgguucugga gccgcaccgg caaggacaac     3180

cccaaguccu ucgagaagca caagaucgag cugaagcccu acgagaaggc ccgguucgag     3240

aagggggagg agcucaaggu gcugggcaag gugcccccga gcuccaacca gcugcagaag     3300

aacaugcaga ucgagaaccu cagcaucuac aaggucaaga cggacgugcu gggcaacaag     3360

cacuucauca agaaggaggg ggacgagccc aagcugaagu ucuga                     3405


<210>  1673
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1673
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaagaacu ccgggaagau cauccacgag aacagccgcc ucuuccccgc cgcgaccgcc      120

gacaacaacg uggagcggcg caagaaccgg caggcccgcc ggcugaaccg ccggaagaag      180

caccgcggcg uccgguucca ggaccuguuc gaggacuacg gccuccugac cgacuucucc      240

aaggugagca ucaaccugaa ccccuaccgc cuccgggugg acgggcugaa ccagcagcug      300

acgaacgagg agcuguucau cgcccucaag aacaucguca agcgccgggg caucuccuac      360

cuggacgacg ccagcgagga cgggggcacc gugcccuccg acuacgggaa ggcggugaag      420

gagaaccgca agcuccuggc cgagaagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc agcuccgcgg ggacuucacc gucgaggaga acggcgagaa gugccggcug      540

aucaacgugu ucagcacguc cgccuacaag aaggaggccg agcgcauccu gcggaagcag      600

caggaguuca acaaccagau caccaacgag uucaucgagg acuaccucaa gauccugacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccggaccga cuacgggcgc      720

uuccgcacgg acggcaccac ccuggacaac aucuucggga uccucaucgg caagugcacc      780

uucuacccgg aggaguaccg ggugagcaag gcgagcuaca cggcccagga guucaacauc      840

cugaacgacc ugaacaaccu caccgucccc accgagacca agaagcuguc cgaggagcag      900

aagaaggaca ucguggagua cgccaaggag acgaagaccc ugggggccag cacccuccug      960

aaguacaucg ccaagaugau cgacgcgucc guggaccaga uccgcggcua ccgggucgac     1020

gugaacaaca agcccgagau gcacaccuuc gagguguacc gcaagaugca gagccuggag     1080

acgaucuccg ucggcgagcu cagccggaac gugcuggacg agcuggccca cauccucacc     1140

cugaacaccg agcgcgaggg gaucgaggag gccaucaaca ccaagcugau gggcguguuc     1200

ucccaggacc agguccucga gcuggugcag uuccggaaga acaacagcag ccuguucucc     1260

aagggguggc acaacuucag ccucaagcug augauggagc ugauccccga gcucuacgag     1320

acguccgagg agcagaugac cauccugacc cgccugggca agcagaaguc caaggagacc     1380

agcaagcgga cgaaguacau cgacgagaag gaggugaccg aggagaucua caacccgguc     1440

guggccaaga gcguccgcca ggccaucaag aucaucaacg aggcgaccaa gaaguacggg     1500

aucuucgaca acaucgugau cgagauggcc cgggagaaca acgaggagga cgccaagaag     1560

gacuacauca agcgccagaa ggccaaccag gacgagaaga acgccgcgau ggagaaggcc     1620

gccuuccagu acaacggcaa gaaggagcuc cccgacucca ucuuccacgg ccacaaggag     1680

cuggccacca agauccggcu guggcaccag cagggggaga agugccugua cacgggcaag     1740

aacaucccca ucagcgaccu cauccacaac caguacaagu acgagaucga ccacauccug     1800

ccccuguccc ucagcuucga cgacucccug agcaacaagg ugcugguccu cgccaccgcg     1860

aaccaggaga aggggcagcg caccccguuc caggcccugg acuccaugga cgacgccugg     1920

agcuaccggg aguucaaguc cuacgugaag gacagcaagc ugcucuccaa caagaagaag     1980

gacuaccugc ugaccgagga ggacaucagc aagaucgagg ugaagcagaa guucaucgag     2040

cgcaaccucg ucgacacgcg guacuccagc cgcguggugc ugaacgcccu gcaggacuuc     2100

uacaaggcgc acaaguucga caccaccauc agcgucgugc ggggccaguu caccucccag     2160

cuccgccgca agugggggcu ggagaagucc cgggagaccu accaccacca cgccguggac     2220

gcccugauca ucgccgccag cagccagcuc cgccugugga agaagcagaa caacccccug     2280

aucacguaca aggagggcca guucgucgac uccgagaccg gcgagaucau cagccucacc     2340

gacgacgagu acaaggagcu gguguucaag gcgcccuacg accacuucgu ggacacccug     2400

cgguccaaga cguucgagga cagcauccuc uucuccuacc aggucgacag caaguucaac     2460

cgcaagaucu ccgacgccac caucuacgcc acccggaagg ccaagcugga caaggagaag     2520

aaggaguaca ccuacacgcu ggggaagauc aaggacaucu acagccucgg caccaagacc     2580

cccuccaaga ccggguucua caaguuccug gaccuguaca acaaggacaa gagccaguuc     2640

cucauguucc agaaggaccg caagacgugg gacgagguga ucgagaagau cauggagcag     2700

uaccggccgu ucaaggagua cgacaagacc ggcaagcugg uggacuucaa ccccuucgag     2760

aaguaccgcc aggagaacgg ccccaucugc aaguacucca agaaggggaa cggccccgag     2820

aucaagagcc ugaaguacua cgacauccuc cuggggaagc acaagaacau caccccggag     2880

ggcucccgga acaccgucgc ccugcucagc cugaaccccu ggcgcacgga cguguacuac     2940

aacauggaga ccaagaagua cgaguuccug gggcucaagu acgcggaccu gcccuucgag     3000

aagggcggcg ccuacgggau cagcaccgag accuacaaca agcugcggga gaaggagggc     3060

aucgggaaga acuccgaguu caaguucacg cucuacaaga acgaccugau ccugaucaag     3120

gacaccgaga ccaacugcca gcagaucuuc cgcuucugga gccggaccgg caaggacaac     3180

cccaaguccu ucgagaagca caagaucgag cucaagccgu acgagaaggc ccgcuucgag     3240

aagggggagg agcuggaggu gcugggcaag gucccgccca gcuccaacca guuccagaag     3300

aacaugcaga ucgagaaccu cagcaucuac aaggugaaga cggacgugcu gggcaagaag     3360

cacaucauca agaaggaggg ggacgagccc aagcugaagu ucuga                     3405


<210>  1674
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1674
augaaguaca ucgagagcgu guccaucggc uucgacaucg gggucggcag cgugggguac       60

gccgugauca acuccaagac caagcagauc cuggagagcg gcgucuccau cuuccccgcc      120

gcgguggccc agaagaacgu ggagcgccgg gagagccgcc agucccggcg ccuccggcgc      180

cggcgcguca accggcugaa cgaccugaag aagcuccuga agaagaacgg guucaucccg      240

agccccaaca agaaccccua cgagauccgc gugcggggcc uggagcacca gcucucccgc      300

gacgagaucg ccaucgccgu gcugcacauc gccaagcggc gcggcaucag cuacucccug      360

gacgacgucg uggacgagaa cgugaccgac uucaagggga agaucgcgaa gaaccaggag      420

gcccucaaga acaagacgcc cggcgagauc caguacgagc ggcugcugaa guacgggaag      480

guccgcggca ccgugaccaa cuacguggag uacgacgagc agccggagua cuuccucaac      540

gucuucccca acagcgccua cguggacgag gccaagcgga uccugaccug ccagaagcag      600

uacuaccccg agaucacgga cgaccugauc gagaacaucg ugaagaucau cucccgcaag      660

cgggcguacu acaucgggcc cggcaacgag aagucccgca ccgacuacgg caucuaccgg      720

accaacgggg agacccucga gaacaucuuc gagauccuga ucggcaagga cagcgucaac      780

ccggacgagc ugcgcgcccc cgccaacagc cucacggccc agguguacaa ccugcugaac      840

gaccucacca acauccgcua caacgggucc gagcggcuga cccccgagca gaagcaggac      900

auccugagcg agcuccugac cacggagaag accgugcaga ugcugaacgu caucgccaag      960

aaggugaacg ugaagaagga ggagaucucc ggcuuccgca ccaagaagga cgacaagccc     1020

gacuuccaca cccucgaggu cuaccggaag gugcgcaagc acaugcugaa caagggcauc     1080

gacaucaacg aguggccgac ggagugcuuc gacgagcugg cguucaucuu caaccucaac     1140

accgagaucg gggagauccg gaaccgcauc agcgagaagg ucgugcccaa guacgacuuc     1200

aucaccaacc agaucgugga cgaccugcug gaggacccca agaucuucgc caucagcacc     1260

ucccagaagu ggcaccgguu cagcaucaag acgaugaacg uccucauccc cgagcugcug     1320

aacggcuaca acgagcagau gaccauccuc acccgcaugg ggcugcugga ggacuccaag     1380

aacgacuaca gcaacaucga caagaucgac aucaagaacg ugaccgagaa caucuacaac     1440

ccgguggucc ggaaguccau caagaagugc cucaagaucu ucaacgagcu guacaagaag     1500

uaccccaaca ucagcgacgu ggugaucgag cugccccgcg aggacaaccc cgaggacgcc     1560

cggaagcuca ucaagaagau gcagaaggag aaccuggucg agaaggacac gucccuggag     1620

gccuuccgca aggaggccaa ccucagcgag aacggccuga ucgacuuccu gcggaagggg     1680

aagaaccgcg cgcucaagau ccggcugugg uaccagcagg agggcaagug ccuguacacc     1740

ggcgagccga uccuggccga ggaccucugc aagaacgagg ccaacuacga caucgaccac     1800

aucaucccca ucuccgugag cuucgacgac uccaucaaca acaaggugcu gugcagcucc     1860

cacuucaacc gcuucgagaa ggagaagaag acccccuucg aguacuucag caacaagucc     1920

agccaccugg ggcgguacuc caacuucgac gaguuccgcg ccagcgucaa cgccaacaag     1980

cggaucuccg acgugaagcg caagaaccuc cugagcaagg agcugcucag cgaccuggac     2040

acccgcaaga aguucaucgc gcggaaccug guggacacgc gcuacgccuc ccgggucgug     2100

uucaacgagc ucacccaguu cuucgccagc aagaaggccg acaccaaggu ggccgucauc     2160

cgcggcaagu ucaccuccca gcugcggcgc gcguggcagc ugaacaagag ccgggacacg     2220

uaccaccacc acgccgugga cgccgcccuc aucgggguga cccccuuccu gaagaucugg     2280

cgcuccggca agaccaucua cccgcggaag augaccgagg cgcagacgga cuucgagcug     2340

aacgagauca ccaccaacga cgaguucaag gagagcuacu accagcuccc cugcccccag     2400

uuccuggacu accugaccca gaugcaggag aaccucaaga ucaacuucga ggucgacaag     2460

aagaugaacc gcaagaucuc cgacgccaug aucuacagcu cccggaagaa gaagcugagc     2520

aaggacaaga aggagaagga guacucccug gggaccauca acgacaucua cagcgacgcc     2580

ggcuacucca aguucaagaa caucuacaag aaggacaagg agaaguuccu cauguaccac     2640

cuggaccaca agagcuucga gauccuggag gacaucaucu cccaguaccc cgacaaggag     2700

gaguacaucg acagcgaggg caaggugaag cagcgcgccg uguccccguu cgagaucuac     2760

cggcgcaagu acggguacau ccggaaguac agcaagaaga acaacggccc caucaucaag     2820

aacaugaagu acuacaacga gaaccucccc aagaucuaca ucgacaucac gccgaagcag     2880

agcaucgaca agaaggucua ccugaccaac aucucccccu ggcgcaccga cguguacuac     2940

aaccggagcc agaacuccua ccacaucaug gggaucaagu acagcgaccu gcgcuacauc     3000

gacggcaagu acgggauccc caagaacgug uacgagaaga ucaagcuccg ggagaagauc     3060

cccuccgaga gcgaguucug cuucucccug uacaagaacg accgcgucaa gaucaucgac     3120

ccgaacaaga acgaggagau cgagcugcgg uucaacagcc gcacccuccc cuccgugcug     3180

aacuacgugg aguucaagcc ccuguuccag gagagcuaca acucccucac gcugcugccc     3240

gucuucagca agaccaccuc cgagggccgc aucaucaaga aguucacccg ggaggacaac     3300

aucguguaca aggugaacac ggacgagcuc ggcaacccgu acuacauccu gaaggagagc     3360

cagaacccca agaacaucau cgacaacucc aacaagaccg aguga                     3405


<210>  1675
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1675
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgagggggu cggcauccug       60

gagaagaacu ccgggaagau cauccacgcc aacagccgcc ucuuccccgc ggccaccgcc      120

gacaacaacg uggagcggcg caagaaccgg caggcccgcc ggcugaaccg ccggaagaag      180

caccgcggcg ugcgguucca ggaccuguuc gaggacuacg gccuccugac cgacuucucc      240

aaggucagca ucaaccugaa cccguaccgc cuccgggugg acgggcugaa ccagcagcug      300

acgaacgagg agcuguucau cgcccucaag aacaucguga agcgccgggg caucuccuac      360

cuggacgacg cgagcgagga cgggggcgcc gucuccagcg acuacgggaa ggccguggag      420

gagaaccgca agcuccuggc cgagcagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc aggugcgcgg ggacuucacc gucguggaga acggcgagaa gcuccggcug      540

aucaacgugu ucuccaccag cgcguacaag aaggaggccg agcgcauccu gcggaagcag      600

caggaguuca acaaccagau cacggacgag uucaucgagg acuaccucaa gauccugacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccggaccga cuacgggcgc      720

uuccgcaccg acggcacgac ccuggacaac aucuucggga uccucaucgg caagugcacc      780

uucuaccccg aggaguaccg ggccagcaag gccuccuaca ccgcccagga guucaaccug      840

cugaacgacc ucaacaaccu gacggucccg accgagacca agaagcugag cgaggagcag      900

aagaagguga ucguggagua cgcgaaggag gccaagaccc ucggggccuc cacgcugcug      960

aaguacaucg ccaagaugau cgacgccagc gucgaccaga uccgcggcua ccggguggac     1020

gugaacaaca agcccgagau gcacaccuuc gaggucuacc gcaagaugca gucccucgag     1080

accaucagcg ugggcgagcu gucccggaac gugcuggacg agcucgcgca cauccugacc     1140

cugaacacgg agcgcgaggg gauggaggag gccaucaaca ccaagcucaa ggacagcuuc     1200

agccaggacc agguccugga gcuggugcag uuccggaaga acaacuccag ccucuucucc     1260

aagggcuggc acaacuucag ccugaagcug augauggagc ucauccccga gcuguacgag     1320

accuccgagg agcagaugac cauccugacg cgccucggga agcagaaguc caaggagacc     1380

agcaagcgga ccaaguacau cgacgagaag gagcugaccg aggagaucua caaccccgug     1440

gucgccaaga gcgugcgcca ggccaucaag aucaucaacg aggccacgaa gaagcacggc     1500

aucuucgaca acaucgucau cgagauggcg cgggagaaca acgaggagga cgccaagaag     1560

gacuacauca agcgccagaa ggccaaccag gacgagaaga acgccgccau ggagaaggcg     1620

gccuuccagu acaacgggaa gaaggagcug ccggacaaca ucuuccacgg ccacaaggag     1680

cuggccacca agauccggcu cuggcaccag cagggcgaga agugccugua caccgggaag     1740

accaucccca ucuccgaccu gaucuacaac caguacaagu acgagaucga ccacauccuc     1800

ccccugagcc uguccuucga cgacagccuc uccaacaagg ugcuggcccu ggcgacggcc     1860

aaccaggaga agggccagcg cacccccuuc caggcccucg acagcaugga cgacgccugg     1920

uccuaccggg aguucaagag cuacgugaag gaccugaagu cccugagcaa caagaagaag     1980

gacuaccucc ugaccgagga ggacaucucc aagaucgagg ucaagcagaa guucaucgag     2040

cgcaaccugg uggacacccg guacagcagc cgcguggucc ucaacgcccu gcaggacuuc     2100

uacaaguccc accaguucga cacgaccauc agcguggugc gggggcaguu caccucccag     2160

cugcgccgca aguggggccu cgagaagucc cgggagaccu accaccacca cgcggucgac     2220

gcccugauca ucgccgccag cagccagcug cgccucugga agaagcagaa caacccgcug     2280

aucaccuaca aggaggggca guucguggac ucccagacgg gcgagaucau cagccugacc     2340

gacgacgagu acaaggagcu cguguucaag gcccccuacg accacuucgu cgacacccug     2400

uccagcaaga ccuucgagga cuccauccug uucagcuacc agguggacuc caaguucaac     2460

cggaagauca gcgacgcgac gaucuacgcc acccgcaagg ccaagcucga caaggagaag     2520

aaggaguaca ccuacacccu gggcaagauc aaggacaucu acucccuggg gacgaagacc     2580

cccagcaaga ccggcuucua caaguuccuc gaccuguaca acaaggacaa gucccaguuc     2640

cugauguucc agaaggaccg gaagaccugg gacgagguga ucgagaagau cauggagcag     2700

uaccgccccu ucaaggagua cgacaagacg gggaagcucg ucgacuucaa cccguucgag     2760

aaguaccggc aggagaacga gcccauccgc aaguacagca agaagggcaa cggccccgag     2820

aucaagagcc ugaaguacua cgacauccug cucgggaagc acaagaacau cacccccgag     2880

ggcucccgga acaccguggc ccugcugagc cucaacccgu ggcgcaccga cguguacuac     2940

aacauggaga cgaagaagua cgaguuccug gggcugaagu acgccgaccu ccccuucgag     3000

gagggcgggg cguacggcau cuccaccgag accuacaacg agcugcggga gaaggagggc     3060

aucgggaaga acagcgaguu caaguucacc cuguacaaga acgaccucau ccugaucaag     3120

gacacggaga ccaacugcca gcagaucuuc cgcuucuggu cccggaccgg caaggacaac     3180

cccaagagcu ucgagaagca caagaucgag cugaagcccu acgagaaggc ccgcuucgag     3240

aagggggagg agcucgaggu ccugggcaag gugccgcccu ccagcaacca gcugcagaag     3300

aacaugcaga ucgagaaccu cuccaucuac aaggugaaga ccgacguccu ggggaacaag     3360

cacuucauca agaaggaggg cgacaagccc aagcugaagu ucuga                     3405


<210>  1676
<211>  3408
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1676
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc ggcccaggcg      120

gagaacaacc ucgagcggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgccggg uccgccugaa ccaccucuuc gaggaguccg gccugaucac cgacuucacc      240

aaggugagca ucaaccugaa cccguaccag cuccggguga aggggcugac cgccgagcug      300

uccaacgagg agcuguucau cgcccucaag aacaugguca agcaccgcgg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgguaccag      480

aaguacggcc agcugcgcgg cgacuucacc gucgaggagg acgggcggaa gcaccgccuc      540

aucaacgugu uccccaccuc cgcguaccac gccgaggccc ugcggauccu gcagacgcag      600

caggaguuca acccccagau caccgacgag uucaucaaca gcuaccucga gauccugacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggaccga cuacggcaag      720

uacacgacca agaaggacgc ccaggggcag uacaucaccc ugaacaacau cuucggcauc      780

cucaucggga agugcaccuu cuaccccgag gaguaccgcg cggccaaggc cagcuacacg      840

gcccaggagu ucaaccugcu gaacgaccuc aacaaccuga ccgugcccac cgagaccaag      900

aagcuguccg aggagcagaa guaccagauc aucacguacg ucaagaacga gaaggccaug      960

ggccccgcga agcuguucaa guacaucgcc aagcuccugg ccugcgacau cgccgacauc     1020

aagggguacc ggaucgacaa gagcgacaag gccgagaucc acaccuucga ggcguaccgc     1080

aagaugaaga cccucgagac caucgacauc gagcagaucg agcgcgagaa gcuggacaag     1140

cuggccuacg ugcucacgcu gaacaccgag cgggagggca uccaggaggc ccuggaccac     1200

gaguucgccg acggcaccuu cucccaggag caggucgacg agcucgugca guuccgcaag     1260

gccaacagcu ccaucuucgg gaagggcugg cacagcuuca gcgugaagcu gaugauggag     1320

cugaucccgg agcucuacgc gaccuccgag gagcagauga cgauccugac ccggcugggg     1380

aagcagaaga ccaccagcuc cagcaacaag acgaaguaca ucgacgagaa gcagcucacc     1440

gaggagaucu acaaccccgu ggucgccaag uccgugcgcc aggccaucaa gaucgugaac     1500

gccgccauca agaaguacgg cgacuucgac aacaucguca ucgagauggc gcgggagacc     1560

aacgaggacg acgagaagaa ggccauccag aagauccaga aggccaacaa ggccgagaag     1620

gacgcggcca ugcgcaaggc cgccaaccag uacaacggga aggccgagcu gccccacagc     1680

guguuccacg gccacaagca gcuggcgacc aagauccggc ucuggcacca gcagggcgag     1740

cgcugccugu acacggggaa gaccaucucc auccacgacc ugaucaacaa ccccaaccag     1800

uucgagaucg accacauccu gccgcucagc aucaccuucg acgacucccu ggccaacaag     1860

gugcuggucu acgccaccgc caaccaggag aagggccagc ggacccccua ccaggcccuc     1920

gacagcaugg acgacgcgug guccuuccgc gagcugaagg ccuucgugcg ggagagcaag     1980

gcccugucca acaagaagaa ggaguaccuc cugacggagg aggacaucag caaguucgac     2040

guccgcaaga aguucaucga gcggaaccug guggacaccc gcuacgccuc ccgggugguc     2100

cucaacgccc ugcaggagca cuucaaggcg cacaagaucg acaccaaggu gagcgugguc     2160

cgcgggcagu ucaccuccca gcugcggcgc cacuggggca ucgacaagac gcgggacacc     2220

uaccaccacc acgccgugga cgcccucauc aucgccgcga gcucccagcu gaaccugugg     2280

aagaagcaga agaacacccu cgugagcuac agcgaggacc agcugcugga caucgagacc     2340

ggggagcuca ucuccgacga cgaguacaag gagagcgucu ucaaggcccc cuaccagcac     2400

uucguggaca cgcugaaguc caaggaguuc gaggacagca uccuguucuc cuaccaggug     2460

gacagcaagu ucaaccgcaa gaucuccgac gccaccaucu acgccacccg ccaggccaag     2520

gucggcaagg acaagaagga cgagaccuac gugcucggca agaucaagga caucuacgcg     2580

cuggggacga agacccccag caagaccggc uucuacaagu uccuggaccu cuacaacaag     2640

gacaaguccc aguuccuggu guaccagaag gaccggaaga ccugggacga ggucaucgag     2700

aagaucaugg agcaguaccg cccguucaag gagagcgaca agaacgggaa ggagguggac     2760

uucaaccccu ucgagaagua ccggaucgag aacggcccca uccgcaagua cucccggaag     2820

ggcaacgggc ccgagaucaa gagccugaag uacuacgccu ccaagcucgg caaccacauc     2880

gacaucacgc cgaaggacag caacaacaag gugguccugc aguccgugag ccccuggcgc     2940

gccgacgugu acuucaacaa gaccaccggg uccuacgaga uccugggccu caaguacgcc     3000

gaccugcagu ucgagaaggg gaccggcacg uaccggauca gcgaggagaa guacaacgac     3060

aucaagaaga aggagggcgu cgacagcgac uccgaguuca aguucacccu guacaagaac     3120

gaccuccugc ugaucaagga caccgagacc aaggagcagc gccucuuccg guuccugagc     3180

cgcuccaugc ccaacaagaa gcacuacgug gagcugaagc ccuacagcaa ggacaaguuc     3240

gagaagaacg agucccucau cgagauccug gggagcgccg acaaguccgg ccggugcauc     3300

aaggggcugg ccaagagcaa ccucuccauc uucaaggugc gcacggacgu ccugggcaac     3360

cagcacauca ucaagaacga gggggacaag ccgaagcugg acuucuga                  3408


<210>  1677
<211>  3408
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1677
augggcguga ucuuccugua cgagaacauc gucaagcccu ucccgagccg cuacgacaag       60

gccaucauga agcggcucaa ggggcgccag accaugggcu acauccuguc ccuggacauc      120

gggaucgcca gcugcggcua cuccgugcuc gagaccgacg cgcugggggu gcccuacaag      180

aucaagacgc ugaacagcau caucuucacc cgggccgaga accccaagac cggcgccucc      240

cucgccgagc cccgccggaa guaccgcggc gcccggcgcc ggaagcgccg gaccagcuuc      300

cgcaagcacc ggacgcgcca gcuguucauc accuccgggc ugcucagcaa gcaggacauc      360

gcgcgguacc ugaaccagca ggagccgcac gacgacaucu gggagcugcg cgucaagggc      420

cucgacgagc cccugaccaa ccugcagcug uuccaggugc ucuacuacuu cgugacccac      480

cggggguuca aguccaaccg caaggccgag cugaaggaca aggacaucgg ccccguccuc      540

aacagcauca acgccuucaa ggaggagaug cagucccacg acuaccggac gaucggggag      600

cuguacgcca acgacccccg cuuccgggac cgcaagcaca acaaggacua caccaacggc      660

uuccugggca ccauccagcg cagcuggcuc guggacgaga ucaagaugcu gcugaccaac      720

cagcaguccu ucgggaacac gcagcucacc agcgaguuca ucgagcugua ccugaacauc      780

uucaccuccc agcggaacuu cgacgacggc ccggcgagcg ggcccuacuc cggcgaccuc      840

aucgacaaga uggugggccc cgacaccuuc aaccgcagcc ggaagcgcgc gccccgggcc      900

acguacaccu uccaguacuu cuccuuccug agcaagcuga acagccuccg caucgccccg      960

uccaucagcg cccccuucga gacccugacc gucgaccagc gggcccugau cacgaagcgc     1020

cucugggggc ccgacgugaa guccgccgac cugaaguuca agcagauccg gauggugcug     1080

agccuccccg agaccgcccg cuucaaccug gucaacuacg gcaacacccc ggagaaggcc     1140

cuggagaucg aggacaagac cacguucauc uccaucaagc cccuccggga gaucgagaag     1200

gcccugaccg ggaccacccu gcccgugagc cacgaccagc ucgacgcgau cgcccgcauc     1260

cugacggugu acaaguccga cagcaaccgg aagaccgccu uccacaacca gaccguccug     1320

uccgacgagg ugaucgagca gcuccugccc cugaacuuca gcaaguucgg ccaccucucc     1380

guggagacca ugcaggagau caucccguac cuggagcagg ggauggucua cagcgacgcc     1440

gccacggcgg ccggcuacga cuuccgcaac aaccagaucg acauggagua cauccgggac     1500

aacgugacca accccguggu ccgccgggcc guguccgugg ccaacaagcu ggucaaggcc     1560

gugguccgcc gguacggcaa gcccgacagc auccacaucg agcuggcgcg cgaccucucc     1620

cagaacuucc aggagcgccg gcaggacgag gugcgccagc agaagaacgc ccgggacaac     1680

gagaagaucg ccaagcgccu ggaggagcug gggauccccg ugaacggcgu caacaucacc     1740

aagaugaagc ucuacgacga gcagcacggg aucgaccugu acgaccugag caccaacgcc     1800

uccauggcgg ugacggacau cuucagcgac gaccgguacg agaucgacca caucaucccg     1860

uacuccguga gcuucgacga cagcuacacc aacaaggucc ucguguccgc caaggccaac     1920

cgcgagaagc gggaccgcau ccccauggag guguucggca acgacagcga gcggcgccgg     1980

caguucgaga accgcgucaa caccaacauc accaaccugc ggaagaagcg caaccugcuc     2040

aaggagaccu ucuccgacga ggacaaggcc aaguggaagu cccggaacau caacgacacg     2100

cgcuaccuga accggauccu gaugaacuac cucagccaga acguggaguu caccgacgug     2160

cugagcgacg ccaagcacgu caucgcgcug aacggggccg ugaccgccaa gauccgcucc     2220

cgguggggca ucaccaaggu gcgcgccgcc ggcgaccucc accacgcggu cgacgccgug     2280

gugaucgggu gcgucacgga cgcccugauc caggccguga cccgguacgc caaguaccag     2340

gagcgccgcg acaacaaggc ggccgccgag caggaccugg ccgagaacaa ccccgaggag     2400

cucgacgcga agcaguuccg gaagguguuc aacgacuacc cccugccgug gcccgaguuc     2460

cgcgaggagc ugaugggccg ggucagcucc gaccccagcc agaucauggc cgggcgccag     2520

uggucccacu acaccgccga ggagaucagc gagcucaagc agccguucgu ggugcggguc     2580

cccaaccacc gcaucguggg ccccgccacc cuggacacgg uguacgggca gcccaacccg     2640

gacgacggcc ugaucaccca gcggguccgc aucaccgacc ucaagcccca gaagaacaag     2700

gacggcgagg acaucaucaa ggccggggcg aacguguacg agcuggccgc cgacggcggg     2760

aacaagcugg uguacgaguc cauccucgac gcccugcagg ucgccggcgg cgacgggaag     2820

aaggcguucc ccaccggccg gcugcucgug cgcagcccca uccaggacau cccgguggag     2880

cggguccgcg ugacguccaa ggccacccug gugguccccg uggggcccaa gagcguggcc     2940

gccaacggcu ccaugguccg gaucgacgug uacaagaccc ccaagaagua cguguucguc     3000

ccgaucuacg ugcgcgacac cgugaaggcc gagcugcccg accgggcggu cgcccccgug     3060

aagcccuaca gccaguggua ccagcuccag ccgaaggacc aguuccuguu cagccuguuc     3120

gccaacgacc ucauccgcuu caagcacccc cgcggggugu ccacgaagga gauccugacc     3180

gacggcagcc aggagaaggg caagcggacc gacgucgugg gguacuucac cggcgccgac     3240

aucgcgacgg ccuccaucgc caucgagggg cccgaccgga gcuucuccgc ccgcggcauc     3300

ggguucgcca gccugaagaa cguggagaag cucaccguca acuacuucgg cgagcugcac     3360

uccgugcacg agacccagcc caugcaguuc cgcggccagc ggggguga                  3408


<210>  1678
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1678
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaaggaca ccgggaagau cauccacgcc uccagccgcc ucuuccccgc ggccaccgcc      120

gacaacaacg uggagcggcg cuccaaccgg cagggccgcc ggcugaaccg ccggaagaag      180

caccgcagcg uccggcugca ggaccucuuc gaguccuacg gccugcugac ggacuucagc      240

aagcucucca ucaaccugaa cccguaccag cugcgcgugc aggggaugga gaaccagcuc      300

accaacgagg agcuguucau cgcccugaag aacaucguga agcggcgcgg caucagcuac      360

cucgacgacg ccuccgagga cgggggcacc gucagcuccg acuacgggaa ggcgguggag      420

gagaaccggc gccugcugac cgagaagacg cccggccaga uccagcucga gcgguucgag      480

aaguacggcc agcugcgcgg ggacuucacc guggaggaga acggcgagaa gcggcgccug      540

aucaacgucu ucagcaccuc cgccuaccgg aaggaggccg agcgcauccu ccggaagcag      600

caggaguuca acaacaagau caccgacgag uucaucgagg acuaccugac gauccugacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccgcaccga cuacgggcgg      720

uuccgcaccg acggcacgac ccucgacaac aucuucggga uccugaucgg caagugcacc      780

uucuaccccg aggaguaccg ggccagcaag gcgagcuaca ccgcccagga guucaaccug      840

cucaacgacc ugaacaaccu gacggugccg accgagacca agaagcucuc cgaggagcag      900

aagaagacca ucaucgagua cgccaagagc gccaagacgc ugggggccuc cacccugcuc      960

aaguacaucg cgaagaugau cgacgccagc guggaccaga uccgcggcua ccgggucgac     1020

gugaacaaca agcccgagau gcacaccuuc gagguguacc gcaagaugca gagccuggag     1080

accaucaagg ucgaggagcu gccccggaag gugcucgacg agcuggccca cauccugacg     1140

cucaacaccg agcgcgaggg caucgaggag gccaucaacu ccaagcugaa ggacaccuuc     1200

aaccgggacc aggugcugga gcucguccag uuccgcaaga acaacagcuc ccuguucagc     1260

aagggguggc acaacuucuc ccugaagcuc augauggagc ugauccccga gcuguacgag     1320

accagcgagg agcagaugac gauccucacc cggcugggca agcagcgcuc caaggagacc     1380

agcaagcgga ccaaguacau cgacgagaag gagcugacgg aggagaucua caacccggug     1440

guggccaagu ccguccgcca ggcgaucaag aucaucaacg aggccaccaa gaaguacggg     1500

aucuucgaca acaucgugau cgagauggcc cgggagaaca acgaggagga cgccaagaag     1560

aacuacauca agcagcagaa ggccaaccag gacgagaaga acgcggccau ggagaaggcc     1620

gccuuccagu acaacggcaa gaaggagcug cccgacaaca ucuuccacgg gcacauggag     1680

cucgccacca agauccgccu guggcaccag cagggcgaga agugccugua caccggcaag     1740

aacauccaga ucagcgaccu cauccacaac caguacaagu acgagaucga ccacauccug     1800

ccccuguccc ucagcuucga cgacucccug agcaacaagg ugcugguccu cgcgacggcc     1860

aaccaggaga aggggcagcg gacccccuuc caggcccugg acuccaugga cgacgccugg     1920

agcuaccgcg aguucaaguc cuacgugaag gagagcaagc ugcucggcaa caagaagaag     1980

gacuaccugc ugaccgagga ggacaucucc aagaucgagg ucaagcagaa guucaucgag     2040

aagaaccucg uggacacccg guacagcagc cgcguggucc ugaacgcgcu gcaggacuuc     2100

uacaagaagc accaguucga caccacgauc uccguggugc gcgggcaguu caccagccag     2160

cuccggcgca aguggaagcu ggagaagucc cgggacaccu accaccacca cgccgucgac     2220

gcccugauca ucgccgccag cucccagcuc cgccugugga agaagcacaa caacccgcug     2280

aucagcuaca aggacggcca guucguggac uccgagaccg gggagaucgu gagccucucc     2340

aacgaggagu acaaggagcu ggucuucaag gcgcccuacg accacuucgu ggacacgcug     2400

cggagcaaga aguucgagga cuccauccuc uucagcuacc agguggacuc caaguacaac     2460

cgcaagauca gcgacgccac caucuacgcc acccggaagg ccaagcugga caaggacaag     2520

aaggaguaca ccuacacgcu gggcaagauc aaggacaucu acgcccucgg caccaagacc     2580

cccuccaaga ccggguucua caaguuccug gaccuguaca agacggacaa gagccaguuc     2640

cucauguacc agaaggaccg caagaccugg gacgagguca ucgagaagau caucgagcag     2700

uaccggcccu ucaaggagua cgacaagaac ggcaaggagg uggacuucaa cccguucgag     2760

aaguaccgca ucgagaacgg gcccauccgg aaguacuccc gcaagggcaa cggcagcgug     2820

aucaagagca ugaaguacua cgacaaccug cuggggaagu ucgucgacau cacccccucc     2880

gagagcaaga accccguggc gcuccugucc cugaacccgu ggcggaccga cguguacuac     2940

aaccccgaga cgcgcaagua cgaguuccuc ggccugaagu acgccgaccu gugcuucggg     3000

aagggcggga gcuacggcau cuccgagguc aaguacaaca agauccggga gaaggagggc     3060

aucgggaagg agagcgaguu caaguucacc cucuacaaga acgaccugau ccugaucaag     3120

gacaccgaga ccaacugcca gcaguucuuc cgcuucuggu cccggacggg caaggacaac     3180

cccaagagcu ucgagaagca caagaucgag cucaagcccu acgagaaggc caaguucgag     3240

aagggggagg agcuggaggu gcugggcaag gugccgcccu ccagcaacca guuccagaag     3300

aacaugcaga ucgagaaccu cuccaucuac aaggucaaga ccgacgugcu ggggaacaag     3360

cacuucauca agaaggaggg cgacgagccc aagcugaagu ucaagaagug a              3411


<210>  1679
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1679
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

aagaaggaca ccggggagau cauccacacc aacucccgcc ucuucagcgc cgcgacggcc      120

gacuccaaca ucgagcggcg ccggcaccgc ggcggcaagc ggcugacccg ccggaagaag      180

caccgcagca uccggcugca cgaccucuuc gaggacuccg ggcugcugac cgacuucagc      240

aaggugucca ucaaccucaa ccccuaccag cugcgcgucc agggccugga caaccagcuc      300

accaacgagg agcuguucau cgcccugaag aacaucguga agcggcgcgg gaucagcuac      360

cucgacgacg ccuccgagga cggcgggacg gugagcuccg acuacggcaa ggccgucgag      420

gagaaccgga agcugcuggc ggagcagacc ccgggccaga uccagcucga ccgcuucgag      480

aaguacgggc agguccgggg cgacuucacc gugguggaga acggggagaa gcgccggcug      540

aucaacgugu ucaccacgag cgccuacucc aaggaggccg agcgcauccu gcggaagcag      600

caggaguuca acaagaagau caccgacgag uucaucgaga acuaccucac cauccugacc      660

ggcaagcgca aguacuacca cggccccggg aacgagaagu cccggacgga cuacggccgc      720

uacaccacca agaaggaccc cgaggggaag uacaucaccc uggacaacau cuucggcauc      780

cucaucggga agugcacguu cuaccccgac gaguaccgcg ccagcaaggc gagcuacacc      840

gcccaggagu ucaaccugcu gaacgaccuc aacaaccuga ccgugccgac cgagacgaag      900

aagcuguccg aggagcagaa gaagaccauc aucaaguacg ccaagaccgc caagacccuc      960

ggcgccagca cgcugcugaa guacaucgcg aagcucaucg gcgccuccgu cgaccagauc     1020

cacggguacc ggaucgaccc caacaagaag cccgagaugc acaccuucga gaccuaccgc     1080

aagaugcaga gccuggagac caucagcgug gaggagaucc cccggaaggu gcuggacgag     1140

cucgcccaca uccugacgcu gaacaccgag cgcgagggca ucgaggaggc caucaacgcc     1200

acccucaagg acaccuucuc ccaggaccag guccuggagc uggugcaguu ccggaagaac     1260

aacagcuccc ucuucagcaa gggguggcac aacuucuccc ugaagcugau gauggagcuc     1320

aucccggagc uguacgagac gagcgaggag cagaugacca uccugacccg ccucggcaag     1380

cagaagucca aggagaccag caagcggacg aaguacaucg acgagaagga gcugaccgag     1440

gagaucuaca accccguggu cgcgaagucc gugcgccagg ccaucaagau caucaacgag     1500

gccaccaaga aguacgggau cuucgacaac aucgugaucg agauggcccg ggagaacaac     1560

gaggaggacg ccaagaaggg cuacaacgag cgccagaagg cgaaccugga cgagaagaag     1620

uccgccaugg agaaggccgc cuuccaguac aacggcaaga aggagcugcc cgacaacguc     1680

uuccacgggc acaaggagcu cgccaccaag auccggcugu ggcaccagca gggcgagaag     1740

ugccuguaca ccgggaagaa cauccccauc agcgaccuca uccagaacca guacaaguac     1800

gagaucgacc acauccugcc gcugagccuc uccuucgacg acagccuguc caacaaggug     1860

cuggugcucg cgacggccaa ccaggagaag ggccagcgca cccccuucca ggcccuggac     1920

agcauggacg acgccugguc cuaccgggag uucaagagcu acgucaagga ccugaagcuc     1980

cuguccaaca agaagaagga cuaccugcuc accgaggagg acaucagcaa gaucgaggug     2040

aagcagaagu ucaucgagcg caaccugguc gacacccggu acagcucccg cguggugcug     2100

aacgcgcucc aggacuucua caaggagcac caguucgaca cgaccaucag cgucgugcgg     2160

gggcaguuca ccucccagcu gcgccggaag uggggccugg agaagucccg cgagaccuac     2220

caccaccacg ccguggacgc ccucaucauc gccgccagca gccagcugcg ccuguggaag     2280

aagcagaaca acccccucau cuccuacaag gagggccagu ucgucgacca ggagacgggg     2340

gagaucauca gccuguccga cgacgaguac aaggagcugg uguucaaggc gcccuacgac     2400

cacuucgugg acacccucaa gagcaagaag uucgaggacu ccauccuguu cagcuaccag     2460

gucgacucca aguacaaccg gaagaucagc gacgccacca ucuacgccac ccgcaaggcc     2520

aagcuggaca aggagaagaa ggaguacacg uacacccucg gcaagaucaa ggacaucuac     2580

gcccuggggu ccgagaagcu gaagaccggc uucuacaagu uccuccaccu guacaagaag     2640

gacaagagcc aguuccugau guaccagaag gaccggaaga ccugggacga ggugaucgag     2700

aagauccucg agcaguaccg cccguucaag gagaaggaca agaacggcaa ggagguggac     2760

uucaaccccu ucgagaagua ccggaucgag aacgggccca uccacaagua cucccgcaag     2820

ggcaacgggc ccgucaucaa gagccugaag uacuacgaca agaacauccc ggagagcuuc     2880

aucaacauca cgcccuccaa cagcaagaac aaggugaucc ugcggcagcu caaccccugg     2940

cgcaccgacg uguacuucaa ccaccagacc gagaaguacg agcugcuggg ccucaaguac     3000

gcgcaccucc aguucgagaa gaagaccggg aacuacucca ucacgaucga caaguacaac     3060

gagaucaagg agaaggaggg cgucagcgag caguccgagu ucaaguucac ccugcacaag     3120

aacgaccucc ugcugaucaa ggacaccgag accaaggagc agcagcuguu ccgguuccug     3180

agccgcacga agcccaacgu gaagcacuac guggagcuga agccguacuc ccgggacgag     3240

uucgagaaga acgagcgccu caucgagauc cugggcagcg ucgacaaguc cgggcgguuc     3300

gugaagggcc ugggcaagag caaccucucc aucuacaagg ugcgcaccga cguccugggg     3360

aaccagcaca ucaucaagaa ggagggggag gagcccaagc ugaaguucug a              3411


<210>  1680
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1680
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaaggaca ccgggaagau cauccacgcc uccagccgcc ucuuccccgc ggccaccgcc      120

gacaacaacg uggagcggcg cuccaaccgg cagggccgcc ggcugaaccg ccggaagaag      180

caccgcagcg uccggcugca ggaccucuuc gagggcuacg ggcugcugac ggacuucucc      240

aaggugagca ugaaccucaa cccguaccag cugcgcgugc agggcaugga gaaccagcug      300

accaacgagg agcuguucgu cgcccucaag aacaucguga agcggcgcgg gaucuccuac      360

cuggacgacg ccagcgagga cggcgggacc guguccagcg acuacggcaa ggcggucgag      420

gagaaccgga agcuccuggc cgagaagacc cccggccaga uccagcugga gcgcuucgag      480

aaguacgggc agcuccgggg cgacuucacg guggaggaga acggggagaa gcaccgccug      540

aucaacgugu ucuccaccag cgccuaccgg aaggaggccg agcgcauccu gcggaagcag      600

caggaguuca acuccaagau caccgacgag uucaucgagg acuaccucau cauccugacc      660

ggcaagcgca aguacuacca cggccccggg aacgagaagu cccggacgga cuacggccgc      720

uuccgcaccg acgggaccac ccuggacaac aucuucggca uccucaucgg gaagugcacg      780

uucuacaccg aggaguaccg ggcgagcaag gccagcuaca ccgcccagga guucaaccug      840

cugaacgacc ucaacaaccu gaccgucccc acggagacca agaagcuguc cgaggagcag      900

aagaagcuca ucaucgagua cgccaagagc gccaagaccc ugggcgcguc cacccugcuc      960

aaguacaucg ccaagaugau cgacgccagc guggaccaga uccgcggcua ccggguggac     1020

gucaacaaca agccggagau gcacacguuc gagguguacc gcaagaugca gagccuggag     1080

accaucaagg uggaggagcu gccccggaag guccucgacg agcuggccca cauccugacc     1140

cucaacaccg agcgcgaggg gaucgaggag gccaucaacu ccaagcugaa ggacaucuuc     1200

aaccgggacc aggugcugga gcucgugcag uuccgcaaga acaacagcuc ccuguucagc     1260

aagggcuggc acaacuucuc caucaagcug augauggagc ucauccccga gcuguacgag     1320

acgagcgagg agcagaugac cauccugacc cggcucggga agcagcgcuc caaggagacc     1380

agcaagcgga cgaaguacau cgacgagaag gagcugaccg aggagaucua caaccccguc     1440

guggcgaagu ccgugcgcca ggccaucaag aucaucaacg aggccaccaa gaaguacggc     1500

aucuucgaca acaucgucau cgagauggcc cgggagaaca acgaggagga cgccaagaag     1560

gacuacauca agcgccagaa ggcgaaccag gacgagaaga acgccgccau ggagaaggcc     1620

gccuuccagu acaacgggaa gaaggagcug ccggacaaca ucuuccacgg ccacaaggag     1680

cugaccacga agauccggcu cuggcaccag cagggcgaga agugccugua caccgggaag     1740

aacaucccca ucagcgaccu gauccacaac caguacaagu acgagaucga ccacauccuc     1800

ccccuguccc ugagcuucga cgacucccuc agcaacaagg ugcugguccu ggcgaccgcc     1860

aaccaggaga agggccagcg cacccccuuc caggcccucg acuccaugga cgacgccugg     1920

agcuaccggg aguucaaguc cuacgugaag gacagcaagc ugcuguccaa caagaagaag     1980

gacuaccucc ugacggagga ggacaucagc aagaucgagg ugaagcagaa guucaucgag     2040

cgcaaccugg ucgacacccg guacagcucc cgcguggugc ucaacgcgcu gcaggacuuc     2100

uacaagagcc accagcugga caccaccauc uccgucgugc gcgggcaguu caccagccag     2160

cuccggcgca aguggggcau cgagaagucc cgggagacgu accaccacca cgccguggac     2220

gcccugauca ucgccgccag cucccagcug cgccucugga agaagcacag caacccgcug     2280

aucgcguaca aggaggggca guucgucgac uccgagaccg gcgagaucgu gagccugucc     2340

gacgaggagu acaaggagcu cguguucaag gcccccuacg accacuucgu cgacacccug     2400

cggagcaaga aguucgagga cuccauccug uucagcuacc agguggacuc caaguacaac     2460

cgcaagauca gcgacgccac caucuacgcc acgcggaagg ccaagcucga caaggagaag     2520

aaggaguaca ccuacacccu gggcaagauc aaggacaucu acgcgcuggg gaccaagacg     2580

cccuccaaga ccggcuucua caaguuccuc gaccuguaca agaccgacaa gagccaguuc     2640

cugauguacc agaaggaccg caagaccugg gacgagguga ucgagaagau caucgagcag     2700

uaccggcccu ucaaggagua cgacaagaac gggaaggagg ucgacuucaa cccguucgag     2760

aaguaccgca ucggcaacgg ccccauccgg aaguacagca agaaggggaa cggccccgag     2820

aucaaguccc ucaaguacua cgacauccug cuggggaagc acaagaacau cacgcccgac     2880

ggcagccgca acaccguggc ccuccugucc cugaacccgu ggcggaccga cguguacuac     2940

aacagcgaga ccaagaagua cgaguuccuc gggcugaagu acgccgaccu gugcuucgag     3000

gagggcggcg ccuacgggau cuccgagguc aaguacaaga agauccgcga gaaggagggc     3060

aucgggaaga acagcgaguu caaguucacg cucuacaaga acgaccugau ccugaucaag     3120

gacaccgaga ccaacugcca gcaguucuuc cgguucuggu cccgcaccgg caaggacaac     3180

cccaagagcu ucgagaagca caagaucgag cucaagcccu acgagaaggc caaguucgag     3240

aagggggagg agcugaaggu gcugggcaag gugcccccgu ccagcaacca guuccagaag     3300

aacaugcaga ucgagaaccu cuccaucuac aaggucaaga cggacauccu gggcaacaag     3360

cacuucauca agaaggaggg ggacgagccc aagcugaagu ucaagaagug a              3411


<210>  1681
<211>  3420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1681
augagcgacc uggugcucgg ccuggacauc gggaucggcu ccgucggggu gggcauccug       60

aacaagguga ccggggagau cauccacaag aacagccgca ucuuccccgc cgcccaggcg      120

gagaacaacc ucgagcggcg caccaaccgg cagggccgcc ggcugacgcg ccggaagaag      180

caccgccggg uccgccugaa ccaccucuuc gaggaguccg gccugaucac cgacuucacc      240

aaggugagca ucaaccugaa ccccuaccag cuccggguga aggggcugac cgccgagcug      300

uccaacgagg agcuguucau cgcccugaag aacaugguca agcaccgcgg caucagcuac      360

cuggacgacg ccuccgacga cgggaacagc uccgugggcg acuacgccca gaucgugaag      420

gagaacagca agcagcucga gacgaagacc cccgggcaga uccagcugga gcgguaccag      480

aaguacggcc agcugcgcgg cgacuucacc gucgaggagg acgggcggaa gcaccgccuc      540

aucaacgugu uccccaccuc cgcguaccac gccgaggccc ugcggauccu gcagacgcag      600

caggaguuca acccccagau caccgacgag uucaucaaca gcuaccucga gauccugacc      660

ggcaagcgca aguacuacca cgggccgggc aacgagaagu cccggaccga cuacggcaag      720

uacacgacca agaaggacgc ccaggggcag uacaucaccc ugaacaacau cuucggcauc      780

cucaucggga agugcaccuu cuaccccgag gaguaccgcg cggccaaggc cagcuacacg      840

gcccaggagu ucaaccugcu gaacgaccuc aacaaccuga ccgugcccac cgagaccaag      900

aagcuguccg aggagcagaa guaccagauc aucacguacg ucaagaacga gaaggccaug      960

ggccccgcga agcuguucaa guacaucgcc aagcuccuga gcugcgacgu ggccgacauc     1020

aagggguacc ggaucgacaa guccgacaag gccgagaucc acaccuucga ggccuaccgc     1080

aagaugaaga cccucgagac ccuggacgug aagaagaugg cgcgcgagga gcuggacaag     1140

cucgccuacg uccugacgcu gaacaccgag cgggagggca uccaggaggc ccucgaccac     1200

gaguucgccg acggcaccuu cagccaggag caggucgacg agcuggugca guuccgcaag     1260

gccaacucca gcaucuucgg gaagggcugg cacagcuucu ccgugaagcu gaugauggag     1320

cucaucccgg agcuguacgc gaccagcgag gagcagauga cgauccugac ccggcucggg     1380

aagcagaaga ccaccuccag cuccaacaag acgaaguaca ucgacgagaa gcagcugacc     1440

gaggagaucu acaaccccgu gguggccaag agcguccgcc aggccaucaa gaucgugaac     1500

gccgccauca agaaguacgg cgacuucgac aacaucguga ucgagauggc gcgggagacc     1560

aacgaggacg acgagaagaa ggccauccag aagauccaga aggccaacaa ggccgagaag     1620

gacgcggcca ugcgcaaggc cgccaaccag uacaacggga aggccgagcu gccccacucc     1680

gucuuccacg gccacaagca gcucgcgacc aagauccggc uguggcacca gcagggcgag     1740

cgcugccugu acacggggaa gaccaucagc auccacgacc ugaucaacaa ccccaaccag     1800

uucgagaucg accacauccu cccgcugucc aucaccuucg acgacagccu ggccaacaag     1860

gugcucgucu acgccaccgc caaccaggag aagggccagc ggacccccua ccaggcccug     1920

gacuccaugg acgacgcgug gagcuuccgc gagcugaagg ccuucgugcg ggaguccaag     1980

gcccucagca acaagaagaa ggaguaccug cugacggagg aggacaucuc caaguucgac     2040

gugcgcaaga aguucaucga gcggaaccuc gucgacaccc gcuacgccag ccggguggug     2100

cugaacgccc ugcaggagca cuuccgcacc cacaagaucg acaccaaggu cuccguggug     2160

cgggggcagu ucacgagcca gcuccgccgg cacuggggca ucgagaagac ccgcgacacc     2220

uaccaccacc acgcggucga cgcccugauc aucgccgccu ccagccagcu gaaccucugg     2280

aagaagcaga agaacacccu ggugagcuac uccgaggacc agcugcucga caucgagacg     2340

ggggagcuga ucagcgacga cgaguacaag gaguccgugu ucaaggcgcc cuaccagcac     2400

uucgucgaca cccucaagag caaggaguuc gaggacucca uccucuucag cuaccaggug     2460

gacuccaagu ucaaccgcaa gaucagcgac gccaccaucu acgccacccg gaaggccaag     2520

cuggacaagg agaagaagga guacacguac acccugggca agaucaagga caucuacgcc     2580

cucggcacca agacccccuc caagacgggg uucuacaagu uccuggaccu guacaagaag     2640

gacaagagcc aguuccucau guaccagaag gaccgccgga ccugggacga ggugaucgag     2700

aagauccugg agcaguaccg cccguucaag gagaaggaca agaacggcaa ggaggucgac     2760

uucaaccccu ucgagaagua ccggaucgag aacgggccca uccgcaagua cucccggaag     2820

ggcaacggcc ccgagaucaa gagccugaag uacuacgaca accuccuggg gcgcuucgug     2880

gacaucaccc cguccgagag caagaacccc guggcgcugc ucucccugaa ccccuggcgg     2940

accgacgucu acuacaacac ggagacccgc aaguacgagu uccugggccu caaguacgcc     3000

gaccugugcu ucgagaaggg cgggagcuac gggaucucca aggugaagua caacaagauc     3060

cgggagaagg agggcaucgg caagaacagc gaguucaagu ucacccugua caagaacgac     3120

cucauccuga ucaaggacac cgagacgaac cgccagcaga ucuuccgguu cuggagccgc     3180

accgggaagg acaaccccaa guccuucgag aagcacaagc uggagcucaa gccguacgag     3240

aagacccggu ucgagaaggg cgaggagcug aaggugcugg ggaagguccc gcccagcucc     3300

aaccgccucc agaagaacau gcagaucgag aaccugagca ucuacaaggu gcggaccgac     3360

gugcugggca accagcacau caucaagaac gagggggaca agcccaagcu cgacuucuga     3420


<210>  1682
<211>  3420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1682
augaacuacu acaacggcuu caucaucacc gagaacgcca uggccagcaa gcacgagaac       60

cugggggcgc ucaccuucgg ccuggacauc gggaucgccu ccgugggcug ggccguccug      120

ggggagaacc gcaucaucga ccucggcgug cggugcuucg acaaggccga gacggccaag      180

gagggcgaga gccugaaccu ggcgcgccgg accgcccgcc uccugcggcg ccggcugcgc      240

cggcgcgccu ggcggcucac caagcuggcc cgccugcuca agcgggaggg gcugaucgcg      300

gacaccaacg uguuccagaa gcccuucaac cugaaggacg ccaacgagaa gggccucucc      360

uucuggcagc ugcgcgucga cgcccuggac cggaagcuca gcgccgagga gugggcccgc      420

gugaucuacc accugugcaa gcaccggggg uuccacugga ucucccgcgc ggaggagaag      480

gccgccgagg gcgacagcaa guccgagggc gggaagguga agcagggccu ggccgggacg      540

aagaagcuca ugcaggagaa gaacuaccgg accgccgcgg agaugguccu gaccgaguuc      600

cccgaggccc agcgcaacaa gcagggcgac uacagcaagg cccugucccg gauccuccug      660

ggggacgaga ugacccagcu guucaagcac cagcgcgagc ucggcaaccc ccacgccacg      720

gagaagcugg aggccgcgau ccugggcaac ggggaccaca agagcggccu cuucugggcc      780

cagaaccccg cccuggccgg gaacgaccug cucaagaugc ugggccacug caccuucgag      840

aaggccgagu accgcgugcc caaggcgucc uucaccgccg agcggcacgu guggcugacc      900

cgccucaaca accugcggau cgucguggac gggacgaccc gcccgcugaa cgaggccgag      960

cggcaccucg cccugcugau gcccuaccag caggcgggcg accucaccua caagcagcug     1020

cgcaccgccc ugacgaaggc cggcuaccuc cccgagagcu uccgguacac cgggcuggcc     1080

uacgccuccg gccagaaggc ggacgagaag gccaaggacc ccgagagcgc cccgcuggug     1140

aagcuccccg ccuggcacga gcugcgcaag acccuggaga agaaggagcu ggccuccgag     1200

uggcagaaga ucagcuccga cgcgcucgac gggaagcccc agcugcugga ccagaucgcc     1260

uggguccuca gcguguacaa ggacgacacc gagguggagg ccgagcugug caagcugaac     1320

cuccccaacg ccgagaagau gaucgacgcc cugcuggaca uccgguucga caaguucucc     1380

aaccucagcc ugaaggcgcu gcgccagauc gucccgcuca uggagcaggg ccagcgguac     1440

gacgaggccg uggccaacau ccccgaguac gggcaccaca gccagcugca cgaggugggc     1500

gccggcgagc acaaguaccu gccgcccuuc uacaaggagc gcgacaagac ggggcggaug     1560

gucuucaacg acgacaugga caucccccgc aaccccgugg ugcuccgggc gcugaaccag     1620

gcccgcaagg ucgugaacgc ccugaucaag gaguacggca gcccccacga ggugcacauc     1680

gagauggccc gggaccucuc ccgcccccug gacgagcggc gcgacgucga gaagcugcag     1740

aaggaguucc gcgaccggaa cgagaaggac aaggcccucu ucgcgcagga cuucaacauc     1800

gugggggccg ucaagggcaa ggaguucgag aaguaccagc uguaccgcga gcagcagggg     1860

aagugcgccu acuccaucga gccgauggac auccaccggc uguucgagca gggcuacgug     1920

gagaucgacc acgcccuccc cuacagccgc uccuucgacg acagcaagaa caaccgggug     1980

cugguccugg ccaaggagaa ccgcgacaag ggcaaccgga cccccuucga guaccucggg     2040

ggcaaggaca acuccgagcg cuggcggcag uucguggcgu ucguggaggg gaacaagagc     2100

uaccgccugg ccaagcgguc ccgccugcuc cggaaggacu ucggcaagga cgaggccaag     2160

ggcuuccgcg agcggaaccu gaacgacacc cgcuacaucu gccgguucuu caagaacuac     2220

gucgagcagu accugcagcu ccacgaggac agcgacgcca agcgcugcgu ggugcugucc     2280

gggcagauga ccagcuuccu gcgggcccgc uggggccucg ucaaggugcg cggggacucc     2340

gaccggcacc acgcgcugga cggcgccauc guggccgccu gcagccacuc cauggucaag     2400

cgccugagcg acuacucccg gcgcaaggag cucgagcagg uccgggcggg guucguggac     2460

guggacacgg gcgagaucgu gaaccccgcc auguuccagc agguccagga gcacuucccg     2520

aaccccuggc cccacuuccg cgacgaggug aagcugcggc ugaacgugga cgaccccgcc     2580

cuccugcaca gcgagaugaa ccgcuucggc accuacuccc cggaggcccu gaaggcccuc     2640

cggccccugu ucaucagccg cgcgccccag cggcgcaaca gcggggccgc ccacaaggac     2700

accaucuacg cccagccgga gcggcuggug gcccagggcg gggucaccca gaaggugccc     2760

cucacguccc ugacccugaa ggaccucgac aagcugaucg acccccaccg caacgagaag     2820

cuguacagcg cgauccggau ccgccucgag cagcacggcg ggaagggcga caaggccuuc     2880

cccgccucca acccgcugcg gaagcccgac agcgagggca accccaccgg gcccaucgug     2940

cgcggcguca ccauggugau cgacaagcug uccgggaucc cgauccgggg cgggaucgcc     3000

aagaacgaca gcauggugcg cgucgacgug uucacgaagg ccggcaaguu ccaccucgug     3060

cccgucuacg ugcaccacgc gguggccaag guccugccca accgggccgu gguggccuac     3120

aaggacgaga ccgaguggac ccugaucgac gacggcuucg acuucugcuu cuccauguac     3180

cccaacgacc uccugcgcau caagcugcgc aacgccagcc accucgggua cuacgcgucc     3240

ugcaaccgga gcaccggcgc caucaaccug uggucccacg accgcaacca gcaggucggg     3300

aaggacggca ugaucgagag caucggggug aagacggccc ugaacgugga gaagcucaac     3360

aucgacgucc ugggcaaccg guacccggcc ccgcccgaga agcgccggga ccuggcguga     3420


<210>  1683
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1683
augaagaaca ugaccaaggu gaacgucggc cuggacgugg ggaucagcuc cgccggcugg       60

agcgugauca acgccgagac caaccagauc cucgaguccg ggguccgccu guucagcggc      120

uccgacgcgg acgagaacgu gaaccggcgc aaccagcggc aggggcgccg gcuguuccgc      180

cggcgcaagc uccggaucca ggaggccaag aaguaccugg agaacguguu ccagcugaag      240

gacaucgagc ccaaggacga guggaacccg uacgagaucc gcguccgggc ccucaacgag      300

aaggugagca aggaggagcu guccaucgcc cugcucaaca ucgugaagca ccgcggcauc      360

agcuacgagc uggaggacgu cacggacgag gagggcacca acacccaguc cuuccagagc      420

gggcuggacu acaaccggcg ccuccugaag accaagacgg ugggcgagau ccaguacgac      480

cggcugcaga aguacggcca ggugcgcggg cagauccaga ccgaggacgg gcuccugauc      540

aacaucuucc ccaccaagaa cuacgucgag gagguggagc ggauccugga gacccagaag      600

aaguaccacc ccgagaucga cgacguguuc aucgagaaga ucaucuccuu cgucacgcgc      660

aagcgggagu acuucguggg ccccggcaac gagaaguccc gcaccgacua cgggaucuac      720

cgggagaacg gcgagacccu cgacaaccug uucgagaucc ugaucgggaa ggacaaguuc      780

uacccggagg aguaccgcgc cgcgggcagc agcuacaccg cccagaucuu caaccuccug      840

aacgaccuga acaaccuccg cauccccacg accgaggacc accaccugac caaggcccag      900

aaggaggaga ucaucaccga gcugaagaac acgucccggc ucagcggcgg gaugcugaag      960

cugaucucca aggugaccaa ggcccccaag gaggagauca gcggcuaccg caaggacgag     1020

aaggacaagc ccgaccucca cucccuggcg uacuaccgga agcagaagaa caugcuggag     1080

aaggagggga uccagaucgc cgacuucagc gaggagcucc uggacgagcu ggccaccaug     1140

augacccuca acacggagcc gggcgagauc cgcaagcagc ugaacaagau caccaaccag     1200

uacgacaucc ugacccccga ggucaucgag gugcucguga agaaccacac cgccuucguc     1260

gccacgagca accacaagug gcaccgguuc ucccugaagc ugaugaagga guucaucccc     1320

gagcuccugg agaccagcaa ggagcagucc accauccugu gcgagcgcgg guucauccag     1380

aaccgggcga gcuaccucga cgagaaccac cacuucaacu acgccgagcu gcgcgagaac     1440

cuguacaacc ccguggugcg gaaguccacg ugcgagaugc ucaaggucuu caccgccauc     1500

caggagaagu acccgaacau ccagaacgug aucgucgagc ugccccgcga gaccaacgag     1560

gacgagcaga agcggaagau caagaagauc aacgacaaca acgccaagcg caagaaggag     1620

auccugaagg aggcccaggc gcagagcggc cuguccgagg aggcccucga ggagaagcug     1680

cugaagacca agggccucua ccugaagcug augcucuggu acgagcagga ggggaagugc     1740

guguacagcg gccaggagau cccccugucc gaccugcucc agaacaaggg cggguacgag     1800

guggaccaca ucauccccau cagcaucucc uucgacgacc ggcugugcaa caagguccug     1860

gugcgccagc agcucaacag ccagaagucc aaccagaccc cguucgagua caugacgacc     1920

cacaccgacg ggcaccugag cuuccacgag cugaaggagc ucgugggcaa gaacaagcgg     1980

uucuccaagg agaagcgcga gcugcugcuc gccgagaucu acagcaacga caucgagacc     2040

cggaagcgcu ucaucaaccg gaaccugguc gacacguccu acaccgcccg caucgugcug     2100

aacgcccucc agcaguacaa gaaggagaac cagcugagca ugaacguguc cgucgugcgc     2160

ggcgcgauga ccaacauccu gcggaagaag gugaagcucg acaagacccg cgagaccuuc     2220

caccaccacg ccaucgacgc cagccugaug gccgucaucc ccaacaucga guucuucaag     2280

gagcggaagg aguggcugcc caagagcgcc aacgaggagg ugguggaggu cgaggugucc     2340

gaggaggaga cgaugcccga gaaggaguuc aaggugaaga ugcgccuccc ggagagcuuc     2400

auccaggacg uccagauccu gcagcggaac uccuucgcga aggaggaggu gagcgggccc     2460

cagacccgcg agcggaaccu gaagaucaag uucucccacc agguggacca gaagaugaac     2520

cgcaaggucg ccgacgacac caucuacggc acccggcagg cccagcucgg gaaggacaag     2580

aaggaggaga cguacgugau cggcaagauc aaggacaucu acaacuacga cgaguacgug     2640

cgcuucaaga agcgguacga caagaacaag gagagcuucc ugauguacca ccacgacccc     2700

cagaccuucg agaagcugga ggagauccuc gcccaguacc ccgaguacau cgaggacgag     2760

aaggggaaga agaagaaggu cuccccguuc gcgcuguacc gcgaggagaa cggccccgug     2820

accaaguacg ccaagaagaa caacggcccc gugaucaagc agcugaagua cuacgacagc     2880

aagcucaacc ggaagaucga caucucccac aaguacagca ccaagaaccg ccaggucgug     2940

cugcaguccc ugaagcccua ccggacggac guguaccgca accaggacac cggggaguac     3000

acccucgucg gcaucgacca gaacgacggg aaguucguga acggccacua cggcaucccc     3060

aagagccggu acgaggagau caaggccaac aacaagcugu ccgagcacga cgaguucgug     3120

uucagccugu acaagaacuc cauggucaag gugaucgacg agaacgggga gagcaucgug     3180

guccgcuacc ucucccggag caugccgaac cgcaagaaca acgccgagau caagcccgug     3240

gagaaguacc aguucgagga cggcgaggug gucccggugu acgggaagug ccggaagcag     3300

augcagaccg uguucgucaa gaagggcugg acgcugcuga aggggaacac cgacauccuc     3360

ggcaacaccu acuucgugga gaaggagagc gagaacccca agaagaugau cgacgacaag     3420

uga                                                                   3423


<210>  1684
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1684
augcgcgaga acggcagcga cgagcggcgc cggaacaugg acgagaagau ggacuaccgc       60

aucgggcugg acaucggcau cgccuccgug gggugggccg uccuccagaa caacagcgac      120

gacgagcccg ugcggaucgu ggaccugggc guccgcaucu ucgacaccgc ggagaucccg      180

aagaccgggg agucccuggc cggcccccgg cgcgccgccc ggacgacccg ccggcgccuc      240

cggcgccgga agcaccgccu ggaccggauc aaguggcugu ucgagaacca gggccucauc      300

aacaucgacg acuuccugaa gcgcuacaac auggccgggc ugcccgacgu guaccagcuc      360

cgguacgagg cgcuggaccg caagcugacc gacgaggagc ucgcccaggu gcugcugcac      420

aucgccaagc accggggcuu ccgcagcacc cgcaaggccg agacggcggc caaggagaac      480

ggggccgucc ucaaggccac cgacgagaac cagaagcgga ugcaggagaa gggcuaccgc      540

accguggggg agaugaucua ccuggacgag gccuuccgga ccggcugcuc cuggagcgag      600

aagggcuaca uccugacgcc ccgcaacaag gcggagaacu accagcacac caugcuccgg      660

gccaugcugg uggaggaggu caaggagauc uucuccagcc agcgccggcu ggggaacgag      720

aaggccaccg aggagcucga ggagaaguac cuggagauca ugaccuccca gcgcagcuuc      780

gaccugggcc cggggaugca gcccgacggc aagcccuccc ccuacgccau ggagggcuuc      840

agcgaccggg uggggaagug cacguuccuc ggcgaccagg gggagcugcg cggcgccaag      900

gggaccuaca ccgcggagua cuucguggcc cugcagaaga ucaaccacac caagcucguc      960

aaccaggacg gcgagacgcg gaacuucacc gaggaggagc gccgggcccu gacccugcuc     1020

cuguucaccc agaaggaggu gaaguacgcc gccgugcgca agaagcuggg ccucccggag     1080

gacauccugu ucuacaaccu gaacuacaag aaggcggcca cgaaggagga gcagcagaag     1140

gagaaccaga acaccgagaa ggccaaguuc aucgggaugc ccuacuacca cgacuacaag     1200

aagugccucg aggagcgggu caaguaccug accgagaacg aggugcgcga ccuguucgac     1260

gagaucggca ugauccugac cugcuacaag aacgacgacu cccggacgga gcgccucgcc     1320

aagcuggggc ucgugcccau cgagauggag ggccugcugg ccuacacccc caccaaguuc     1380

cagcaccuca gcaugaaggc gaugcggaac aucaucccgu uccuggagaa ggggaugacc     1440

uacgacaagg ccugcgagga ggccggcuac gacuucaagg ccgacuccaa gggcacgaag     1500

cagaagcugc ucaccgggga gaacgucaac cagaccauca acgagaucac caaccccgug     1560

gugaagcgca gcgucuccca gacggugaag gugaucaacg cgaucauccg caccuacggc     1620

agcccccagg ccaucaacau cgagcuggcc cgggagaugu ccaagaccuu cgaggagcgc     1680

cggaagauca agggggacau ggagaagcgc cagaagaaca acgaggacgu caagaagcag     1740

auccaggagc ugggcaagcu gagccccacc gggcaggaca uccucaagua ccggcugugg     1800

caggagcagc agggcaucug cauguacagc ggcaagacga ucccgcugga ggagcuguuc     1860

aagcccgggu acgacaucga ccacauccuc cccuacucca ucaccuucga cgacagcuuc     1920

cgcaacaagg ugcuggucac cucccaggag aaccggcaga agggcaaccg cacccccuac     1980

gaguacaugg ggaacgacga gcagcggugg aacgaguucg agacccgcgu gaagacgacc     2040

auccgggacu acaagaagca gcagaagcuc cugaagaagc acuucagcga ggaggagcgc     2100

uccgaguuca aggagcggaa ccugaccgac accaaguaca ucacgaccgu gaucuacaac     2160

augauccgcc agaaccucga gauggccccg cugaaccggc ccgagaagaa gaagcagguc     2220

cgcgccguga acggcgcgau caccgccuac cugcggaagc gcuggggccu cccccagaag     2280

aaccgggaga ccgacacgca ccacgccaug gacgccgugg ucaucgccug cugcaccgac     2340

gggaugaucc agaagaucag ccgcuacacc aaggugcggg agcgcugcua cuccaagggc     2400

accgaguucg uggacgcgga gacgggggag aucuuccgcc ccgaggacua cagccgggcc     2460

gagugggacg agaucuucgg cguccacauc ccgaagcccu gggagaccuu ccgcgccgag     2520

cuggacgugc ggauggggga cgaccccaag ggcuuccugg acacccacuc cgacguggcc     2580

cucgagcugg acuaccccga guacaucuac gagaaccugc gcccgaucuu cgucagccgg     2640

augcccaacc acaaggugac cggcgccgcg cacgccgaca cgauccgcag cccccggcac     2700

uucaaggacg aggggaucgu gcucaccaag accgcccuga ccgaccugaa gcucgacaag     2760

gacggcgaga ucgacgggua cuacaacccg caguccgacc ugcugcucua cgaggcccug     2820

aagaagcagc ugcuccugua cggcaacgac gcgaagaagg ccuucgccca ggacuuccac     2880

aagcccaagg ccgacgggac ggagggcccc gucgugcgca aggugaagau ccagaagaag     2940

cagaccaugg gcgucuucgu ggacuccggg aacggcaucg ccgagaacgg gggcauggug     3000

cggaucgacg ucuuccgcgu gaacgggaag uacuacuucg ugcccgucua caccgcggac     3060

guggugaaga agguccugcc gaaccgggcc agcaccgccc acaagcccua cggcgagugg     3120

aaggugaugg aggacaagga cuuccucuuc ucccuguaca gccgcgaccu gauccacauc     3180

aaguccaaga aggacauccc caucaagaug gugaacggcg ggauggaggg caucaaggag     3240

acguacgccu acuacaucgg ggccgacauc agcgcggcca acauccaggg caucgcccac     3300

gacucccggu acaaguuccg cgggcucggc auccagagcc uggacguccu ggagaagugc     3360

cagaucgacg ugcucggcca cgugagcguc gugcgguccg agaagcgcau gggguucagc     3420

uga                                                                   3423


<210>  1685
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1685
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

gagaagaacu ccgggaagau cgugcacgcc agcucccgca ucuuccccgc ggccaccgcc      120

gacaacaacg ucgagcggcg caagaaccgg caggcccgcc ggcucaaccg ccggaagaag      180

caccgcggcg ugcgguucca ggaccuguuc gaggacuacg gccugcucac cgacuucagc      240

aaggugucca ucaaccugaa cccguaccgc cugcgggucg acgggcucaa ccagcagcug      300

acgaacgagg agcuguucau cgcccucaag aacaucguga agcgccgggg caucagcuac      360

cuggacgacg cguccgagga cgggggcacc gugagcuccg acuacgggaa ggccgucgag      420

gagaaccgca agcugcucgc cgagaagacc cccggccaga uccagcugga gcgguucgag      480

aaguacggcc agcugcgcgg ggacuucacc gugguggaga acggcgagaa gugccggcuc      540

aucaacgucu ucagcacguc cgccuacaag aaggaggcgg agcgcauccu gcggaagcag      600

caggacuuca acaaccagau caccgacgag uucaucgagg acuaccugaa gauccucacc      660

gggaagcgca aguacuacca cggccccggc aacgagaagu cccggaccga cuacgggcgc      720

uacacgaccc acaaggacag caagggcgag uacgugaccc uggacaacau cuucggggug      780

cugaucggca agugcaccuu cuaccccaac gaguaccgcg ccagcaaggc cuccuacacg      840

gcccaggagu ucaaccuccu gaacgaccug aacaaccuca ccgucccgac cgagaccaag      900

aagcugagcg aggagcagaa gaagacgauc aucgaguacg ccaaguccgc gaagacccug      960

ggggccagca cccuccugaa guacaucgcc aagaugaucg acaccuccgu ggaccagauc     1020

cggggcuacc gcguggacgu caacaacaag cccgagaucc acacguucga gguguaccgg     1080

aagaugcaga gccuggagac caucagcgug ggcgagcucu cccgcaacgu ccuggacgag     1140

cuggcccaca uccucacccu gaacaccgag cgggagggga ucgaggaggc caucaacacg     1200

aagcugaagg acagcuucuc ccaggaccag gugcucgagc uggugcaguu ccgcaagaac     1260

aacagcuccc uguucagcaa gggcuggcac aacuucuccc ucaagcugau gauggagcug     1320

auccccgagc ucuacgagac cagcgaggag cagaugacca uccugacccg gcuggggaag     1380

cagaagucca aggagacgag caagcgcacc aaguacaucg acgagaagga gcucaccgag     1440

gagaucuaca accccgucgu ggcgaagucc guccggcagg ccaucaagau caucaacgag     1500

gccaccaaga agcacggcau cuucgacaac aucgugaucg agauggcccg cgagaacaac     1560

gaggaggacg ccaagaagga cuacaucaag cggcagaagg cgaaccagga cgagaaguac     1620

gccgccaugg agaaggccgc cuuccaguac aacgggaaga aggagcugcc ggacaacauc     1680

uuccacggcc acaaggagcu ggcgacgaag auccgccugu ggcaccagca gggcgagaag     1740

ugccucuaca ccggcaagag cauccccauc uccgaccuga uccacaacca guacaaguac     1800

gagaucgacc acauccugcc ccucagccug uccuucgacg acagccuguc caacaaggug     1860

cucguccugg ccaccgccaa ccaggagaag gggcagcgga cccccuucca ggcccuggac     1920

agcauggacg acgcgugguc cuaccgcgag uucaagagcu acgugaagga gagcaagcuc     1980

cuggggaaca agaagaagga guaccugcuc acggaggagg acaucuccaa gaucgaggug     2040

aagcagaagu ucaucgagcg gaaccugguc gacacccgcu acagcucccg gguggugcug     2100

aacgcccucc aggacuucua caagaaccac aacuucgaca ccaccaucag cgucgugcgc     2160

ggccaguuca ccucccagcu gcggcgcaag ugggggcugg agaagucccg cgagacguac     2220

caccaccacg ccguggacgc ccucaucauc gccgcgagca gccagcugcg gcuguggaag     2280

aagcagaaca acccgcucau cgccuacaag gagggccagu ucgucgacuc ccagaccggc     2340

gagaucauca gccugaccga cgacgaguac aaggagcugg uguucaaggc ccccuacgac     2400

cacuucgugg acacccucuc cagcaagacg uucgaggacu ccauccuguu cagcuaccag     2460

gucgacucca aguucaaccg caagaucagc gacgccacca ucuacgccac ccggaaggcg     2520

aagcuggaca aggagaagaa ggaguacacc uacacgcucg ggaagaucaa ggacaucuac     2580

ucccugggca ccaagacccc cagcaagacc ggguucuaca aguuccugga ccucuacaac     2640

aaggacaaga gccaguuccu gauguuccag aaggaccgca agacguggga cgaggugauc     2700

gagaagauca uggagcagua ccggcccuuc aaggaguacg acaaggccgg caagcuggug     2760

gacuucaacc cguucgagaa guaccgccag gagaacggcc ccauccggaa guacuccaag     2820

aaggggaacg gccccgagau caagagccuc aaguacuacg acauccugcu ggggaagcac     2880

aagaacauca cccccgaggg cucccgcaac accgucgccc uccugagccu gaacccgugg     2940

cggaccgacg uguacuacaa cauggagacg aagaaguacg aguuccucgg gcugaaguac     3000

gccgaccugc ccuucgagga gggcggcgcc uacgggaucu ccaccgagac cuacaacgag     3060

cuccgcgaga aggagggcau cgggaagaac agcgaguuca aguucacccu guacaagaac     3120

gaccugaucc ucaucaagga cacggagacc aacugccagc aguucuuccg guucuggucc     3180

cgcaccggca aggacaaccc caagagcuuc gagaagcaca agaucgagcu gaagcccuac     3240

gagaaggcga aguucgagaa gggggaggag cuggaggugc ucggcaaggu cccgcccucc     3300

agcaaccagu uccagaagaa caugcagauc gagaaccugu ccaucuacaa ggugaagacc     3360

gacgugcugg gcaacaagca cuucaucaag aaggaggggg acaagcccaa gcucaaguuc     3420

uga                                                                   3423


<210>  1686
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1686
augaacggcc uggugcucgg gcuggacauc ggcaucgcca gcgucggggu gggcauccug       60

aagaaggaca ucggggagau cauccacacc aacucccgcc ucuucagcgc cgcgaccgcc      120

gacuccaaca ucgagcggcg cggccaccgg ggcgggaagc gccugacgcg gcgcaagaag      180

caccggagca uccgccugca cgaccucuuc gaggacuucg gccugcugac cgacuucucc      240

aaggugagca ucaaccucaa ccccuaccag cugcgggucc aggggcugga caaccagcuc      300

accaacgagg agcuguucau cgcccugaag aacaucguga agcgccgggg caucuccuac      360

cucgacgacg ccagcgagga cgggggcacc guguccagcg acuacggcaa ggccgucgag      420

gagaaccgca agcugcuggc ggagcagacg ccggggcaga uccagcucga ccgguucgag      480

aaguacggcc aggugcgcgg ggacuucaac guggucgaga acggcgagaa gcggcgccug      540

aucaacgugu ucaccaccuc cgccuacagc aaggaggccg agcggauccu gcgcaagcag      600

caggaguuca acaagaagau caccgacgag uucaucgagg acuaccucac gauccugacc      660

ggcaagcgga aguacuacca cgggcccggc aacgagaagu cccgcaccga cuacgggcgg      720

uacaccacga agaaggaccc cgagggcaag uacaucaccc uggacaacau cuucgggauc      780

cucaucggca agugcaccuu cuaccccgac gaguaccgcg ccagcaaggc guccuacacc      840

gcccaggagu ucaaccugcu gaacgaccuc aacaaccuga cggugccgac cgagaccaag      900

aagcugagcg aggagcagaa gaagaccauc aucaaguacg ccaagacggc caagacccuc      960

ggcgccucca cccugcugaa guacaucgcg aagcucaucg gggccagcgu cgaccagauc     1020

cacggcuacc gcaucgaccc caacaagaag cccgagaugc acaccuucga gacguaccgg     1080

aagaugcagu cccuggagac caucagcgug gaggagcugc cccgcaaggu gcucgacgag     1140

cuggcccaca uccugacccu caacaccgag cgggagggga ucgaggaggc caucaacgcc     1200

acgcugaagg acaccuucag ccaggaccag guccuggagc ucgugcaguu ccgcaagaac     1260

aacuccagcc uguucuccaa gggcuggcac agcuucuccc ugaagcucau gauggagcug     1320

aucccggagc uguacgagac cagcgaggag cagaugacca uccucacgcg gcuggggaag     1380

cagaagucca aggagaccag caagcgcacc aaguacaucg acgagaagga gcugaccgag     1440

gagaucuaca accccguggu cgcgaagucc gugcggcagg ccaucaagau caucaacgag     1500

gccacgaaga aguacggcau cuucgacaac aucgugaucg agauggcccg cgagaacaac     1560

gaggaggacg ccaagaagga guacaucaag cggcagaagg cgaaccugga cgagaagaac     1620

gccgccaugg agaaggccgc cuuccaguac aacggcaaga aggagcuccc cgacaacguc     1680

uuccacgggc acaaggagcu ggcgaccaag auccgccugu ggcaccagca gggcgagaag     1740

ugccucuaca ccgggaagaa cauccccauc agcgaccuga uccagaacca guacaaguac     1800

gagaucgacc acauccugcc gcucucccug agcuucgacg acucccugag caacaaggug     1860

cucgugcugg ccaccgccaa ccaggagaag ggccagcgga cccccuucca ggcccuggac     1920

uccauggacg acgcguggag cuaccgcgag uucaaguccu acgucaagga cagcaagcuc     1980

cuggggaaca agaagaagga guaccugcuc acggaggagg acaucuccaa gaucgaggug     2040

aagcagaagu ucaucgagcg gaaccugguc gacacccgcu acagcagccg gguggugcug     2100

aacgcccucc aggacuucua caaggagcac caguucgaca ccaccaucuc cgucgugcgc     2160

ggccaguuca cgagccagcu gcggcgcaag uggggccugg agaagucccg ggagaccuac     2220

caccaccacg ccguggacgc ccucaucauc gccgcgagcu cccagcugcg ccuguggaag     2280

aagcagaaca acccccucau cagcuacacc gaggggcagu ucgucgacca ggugaccggc     2340

gagaucaucu cccugagcga cgacgaguac aaggagcugg uguucaaggc ccccuacgac     2400

cacuucgucg acacgcucaa guccaagaag uucgaggaca gcauccuguu cuccuaccag     2460

guggacagca aguacaaccg caagaucucc gacgccacca ucuacgccac ccggaaggcc     2520

aagcuggaca aggagaacaa ggaguacacc uacacgcucg ggaagaucaa ggacaucuac     2580

gcgcugggca ccaagagccc guccaagacc ggcuucuaca aguuccugga ccucuacaac     2640

aaggacaaga gccaguuccu gauguuccag aaggaccgca agaccuggga cgaggugauc     2700

gagaagauca ucgagcagua ccggcccuuc aaggaguacg acgagaacgg gaaggagguc     2760

gacuucaacc ccuucgagaa guaccgcauc gagaacggcc ccauccggaa guacuccaag     2820

aaggggaacg gcccggagau caagagccug aaguacuacg acaaccuccu ggggaaguuc     2880

guggacauca cgcccagcga guccaagaac cccguggccc ugcucagccu gaaccccugg     2940

cgcaccgacg ucuacuacaa caccgagacc uccaaguacg aguuccuggg ccucaaguac     3000

gccgaccugu gcuucgagaa gggcggggcc uacggcauca gcgaggugaa guacaacaag     3060

auccgggaga aggaggggau cggcaaggag uccgaguuca aguucacgcu guacaagaac     3120

gaccucaucc ugaucaagga caccgagacc aacugccagc agaucuuccg cuucuggagc     3180

cggaccggga aggacaaccc gaaguccuuc gagaagcaca agaucgagcu gaagcccuac     3240

gagaaggccc gcuucgagaa gggcgaggag cucgaggugc ugggcaaggu cccgcccagc     3300

uccaaccagc ugcagaagaa caugcagauc gagaaccuca gcaucuacaa ggugaagacg     3360

gacgugcugg ggaacaagca cuucaucaag aaggagggcg aggagcccaa gcugaaguuc     3420

uga                                                                   3423


<210>  1687
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1687
auggaggaga acuuccagua ccgcaucggc cuggacaucg ggaucaccag cgugggcugg       60

gccguccucc agaacaacuc ccaggacgag cccgugcgga ucaccgaccu gggggugcgc      120

aucuucgacg ucgccgagaa cccgaagaac ggcgacgcgc uggccgcgcc ccggcgcgac      180

gcccggacga cccgccggcg ccuccggcgc cggcgccacc ggcuggagcg caucaaguuc      240

cugcuccagg agaacgggcu gaucgagaug gacagcuuca uggagcggua cuacaagggc      300

aaccugcccg acguguacca gcuccgcuac gagggccugg accggaagcu gaaggacgag      360

gagcucgccc aggugcugau ccacaucgcc aagcaccgcg gguuccgguc cacccgcaag      420

gccgagacca agggcaagga gggcggggcc guccugaagg ccacgaccga gaaccagaag      480

aucaugcagg agaaggggua ccggaccgug ggcgagaugc ucuaccugga cgaggcguuc      540

cacaccgagu gccuguggaa cgagaagggc uacgugcuca cgccccgcaa ccgcccggac      600

gacuacaagc acaccauccu gcggagcaug cuggucgagg aggugcacgc caucuucgcc      660

gcccagcgcg cccucgggaa cgagaaggcg accgaggagc uggagaacgc cuacguggag      720

aucaugaccu cccagcggag cuucgacaug ggccccgggc ugcagcccga cggcaagccc      780

uccccguacg ccauggaggg cuucggggac cgcgucggca agugcacguu cgagaaggac      840

gaguaccggg cccccaaggc caccuacacc gcggagcugu ucguggcccu ccagaagauc      900

aaccacacca agcugaucga cgaguucggg acgggccgcu ucuucaccga ggaggagcgg      960

aagaccauca ucgaccuccu gcugagcggg aaggagcuca aguacggcac cauccgcaag     1020

aagcugaaca ucgaccccuc ccugaaguuc aacagccuca acuacuccgc caagaaggag     1080

ggcgagacgg aggaggagcg ggugcgcgac accgagaagg ccaaguucgc cagcauguuc     1140

uggaccuacg aguacuccaa gugccugaag gaccggaacg aggagaugac cgucgggcag     1200

aaggcggacc ugcucgaccg caucggcgag auccugacgg ccuacaagaa cgacgacagc     1260

cgguccagcc gccuggagga ccucgggcug ucccgggagg agaucgacgg ccugcucgag     1320

cugagccccg ccaaguacca gcgcgugucc cugaaggcca ugcggaagau gcagccguac     1380

cucgaggacg ggcugaucua cgacaaggcc ugcgaggcgg ccggcuacga cuuccgcgcc     1440

cugaacgacg gcaacaagaa gcaccuccug aagggggagg agaucaacgc caucgugaac     1500

gacaucacca accccgucgu gaagcggagc gugagccaga ccaucaaggu caucaacgcg     1560

aucauccaga aguacggcag cccccaggcc gugaacaucg agcuggcccg cgagaugucc     1620

aagaacuucc aggaccgcac caaccuggag aaggagauga agaagcggca gcaggagaac     1680

gagcgcgcca agcagcagau caucgagcuc gggaagcaga accccacggg ccaggacauc     1740

cugaaguacc ggcuguggaa cgaccagggc ggguacugcc ucuacuccgg caagaagauc     1800

ccgcuggagg agcuguucgg gggcggguac gacaucgacc acauccuccc cuacagcauc     1860

accuucgacg acuccuaccg caacaaggug cuggucaccg cccaggagaa ccggcagaag     1920

ggcaaccgca cccccuacga guacuucggc gcggacgaga agcacuggga ggacuacgag     1980

gcccgggugc gccugcucgu ccgggacuac aagaagcagc agaagcugcu gaagaagaac     2040

uucacggagg aggagcgcaa ggaguucaag gagcggaacc ucaacgacac caaguacauc     2100

acccgcgugg uguacaacau gauccggcag aaccuggagc uggagcccuu caaccacccg     2160

gagaagaaga agcaggucug ggccgugaac ggggccguga ccagcuaccu ccgcaagcgg     2220

uggggccuga ugcagaagga ccgcuccacg gaccggcacc acgccaugga cgcggucgug     2280

aucgccugcu gcaccgacgg gaugauccac aagaucagcc gcuacaugca gggccgggag     2340

cuggccuacu cccgcaacuu caaguucccc gacgaggaga ccggggagau ccucaaccgg     2400

gacaacuuca cccgcgagca gugggacgag aaguucggcg ugaagguccc ccugcccugg     2460

aacagcuucc gcgacgagcu ggacauccgg cuccugaacg aggacccgaa gaacuuccug     2520

cucacccacg ccgacgugca gcgcgagcug gacuaccccg gcuggaugua cggggaggag     2580

gaguccccca ucgaggaggg ccgguacauc aacuacaucc gcccccuguu cgugagccgg     2640

augccgaacc acaaggucac gggguccgcc cacgacgcga ccauccgcag cgcccgggac     2700

uacgagaccc gcggcguggu gaucaccaag gucccccuca cggaccugaa gcugaacaag     2760

gacaacgaga ucgaggggua cuacgacaag gacuccgacc ggcuccugua ccaggcccug     2820

gugcgccagc uccugcugca cggcaacgac ggcaagaagg ccuucgcgga ggacuuccac     2880

aagcccaagg ccgacgggac cgagggcccg gugguccgga aggugaagau cgagaagaag     2940

cagaccagcg gggugauggu ccgcggcggg accggcaucg ccgccaacgg cgagauggug     3000

cggaucgacg uguuccgcga gaacgggaag uacuacuucg uccccgugua cacggccgac     3060

gugguccgga aggugcuccc caaccgcgcg gccaugaacg gcaagaucgc cuccgacugg     3120

aagaccaugg aggacaacaa cuucguguuc agccuguacu cccgggaccu gauccacguc     3180

aagcgcaaga aggccauccc caccauccuc agcagcgggg ccaccuucga gcaggaggag     3240

uucuacgcgu acuucacggg cgccgacauc uccaccguga gcaucgccgg gaucuccaac     3300

gacaacagcu ucaaguuccg gggccugggc auccaggggc uggagaucuu cgagaagugc     3360

cagguggaca uccucggcaa caucuccguc gugcgccacg agaaccgcca gggguuccac     3420

uga                                                                   3423


<210>  1688
<211>  3426
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1688
auguacagca ucggccugga caucgggauc gccuccgugg gcuacagcgu caucgagacc       60

aacuccgggc gcaucgugga gcucggcgcc cggaucuucg aggcgcgcaa cagcgacaac      120

aacaaggacc ggcgcgacuu ccgcgggggc cggcggcuga uccgccggcg caagacccgg      180

cuggacgacg ccaagcgcua ccucgacgac cagggcuuca agaacgacaa gucccugauc      240

aacgugugcc ccuacagccu gcgggucaag gggcucacgg aggagcuguc caagagcgag      300

auccgcaagg ugauccugca cauccucaag aagcggggcg uguccuaccu gagcuccgag      360

gaccuggaca ccagcuccgu cgacaacaag gacagcuuca agcaggccgu gaccaagaac      420

cuccgccagc uggacgacaa gaccccgggg cagauccagu acgagcggcu gcaggagucc      480

ggccgcgugc ggacggggau caaccagaac ggccaguacc agcucaacgu cuucgacgug      540

gccagcuacg ccaaggagcu gaagcugauc cucgagaccc agcagcagua ccaccaggag      600

aucgacgacc acuuccugga cuucuucacc aaggacggcu accgcgagga cgcggggcug      660

aucuaccgga agcgccccua cuacgacggc cccgggaacg ccgcccagaa cuccagcuac      720

ggccgcuggg ccaacuacca gguggacggc aagcccgcgg agaacaucuu cgaccagcuc      780

aucgggaagg acuucaccgg caagauccgg gccuccaacc uggggccgag cgcccagaag      840

uucaaccugc ucaacgaccu gaacaaccug cgcaucacga cccgggagga ggagaagcuc      900

accuucgagg agaagcagga gauccuggag guccuggaga acgaccgccc caagaccuuc      960

ggcccccgga acaucgccaa gaugcucaag guggacaugg acgacaucaa gggguggcgc     1020

cugaacagcu ccgacaagcc cgagauccac acgaugaagg aguacaacga caugcggcug     1080

gugcucgagg ccggcggcuu cgacaucaag gcgaucgacc uggaccacau cgaggagauc     1140

gccagccuga ucacccucaa caccgagcgc gaggccuucg agcugucccu gagcaacgag     1200

aucgacaaca accucaagca guuccugguc gagaacuuca acgcccugcg gaagugcaag     1260

ccgaacaacu ccuggcacag cuucuccaag gagaaccucc agcgccugau caccgagcug     1320

gagcacacga gcgacgagca gaacaccauc cucgagcggc uggggcugaa guucgaccuc     1380

cgcuccaagu acagcgaccg ggacacccug cccauccacg aggugcugga ggagaucuac     1440

aaccccaccg uguccaagag cguccgccag acguuccggg ugcugaacgc ccucgugaag     1500

aaguacggca aggagaacau cgacuacguc accaucgaga ugccccgcga caagaacgag     1560

gacgaccaga agaagaccau ccgggagauc cagaagguga acaagaaccg caaggacaag     1620

uccaaggagu acuuccugaa gaaguccggg uggagcgagg agaaguucga ggagaagcug     1680

cggaccagcc gccgcuucgc guccaagcuc uacuacuucu acgagcagga cggccugugc     1740

gccuacacgg ggcagcagau ccagccggag gaccugcuca ccgagcgggu ggaggucgac     1800

cacaucaucc cccugagcgu gucccuggac gacagcauca acaacaaggu ccucgugacc     1860

agcgaggcca accagaagaa gggccagcgc ucccccuacg acgccuucau gagcggcgac     1920

uucuccaagu accggaccua cgacgaguac aaggccuggg ugcugagcaa caaguacaag     1980

aaguacaaga aggcgcugcu ccuggaggag cgcaacaucu ucgaccccga gcuggccaag     2040

aaguuccggc agcgcaaccu caacgacacg cgguacgccu cccgcaucgu ccugaacgcc     2100

gugcagagcu ucuucuacaa gaccgacacc aagacccggg uggucaacgg ggccuucacg     2160

cacacccugc gcaagaagug gaaccucgcg gacaaggacc gggagaccca ccaccaccac     2220

gccaucgacg ccacccuggu ggccgugacg ccguucguca aggcggagcg cuacgaguac     2280

uacaaggacu ccgagggcaa ggccagcaug guggacaccg ugaccgggac caucaucccc     2340

uacuccgagu acaagaagaa gaaccuguac gaccggagca acuaccuccu gauguucggc     2400

aacgagucca ccaucaacca ggagaaggag gacaagagcg ccgaguucau ccgcaaccug     2460

auccccgcca agaucuaccc cgagaucaag uucucccacc aggucgaccg caagagcaac     2520

cggcggcucu ccgacgccac gaucuacagc acccgcuccu acaccgugga gaaggggcgg     2580

ggcaagaaca agcgcgaggu gaucgagcgg gacaucaucg gcaagaucaa ggacaucuac     2640

accgcggacg gguacgccga guacgucaag uacaaggaca gccugcuggu gaaggagauc     2700

gacgagaaga cguucgagaa gcucgaggcc aucaucgagg aguucagcga caccguggag     2760

gucaccgacc agcagggcaa ggugaagaag guguccaaga gcccguuccu gcaguacugc     2820

gagaaguacg acguccccgg ggugaccaag uacuccaaga agaacaacgg ccccgugauc     2880

cgcagccuga aguaccgcua cacgaagcuc cggucccaca ucgacaucag ccccaagaac     2940

uccaagaaca ccgucgcccu gcugacccuc gccccguggc gcaccgacgu guacuacagc     3000

caggagacgg cguccuacca ccuccugggc cugaaguaca ucgaccugag cuuccgggac     3060

gggggcuacg gggugucccg caagcgguac gaggagauca agaagaacga gcgcaucccc     3120

gaggacggcg aguucaaguu cagccuguac aagaaggacu acaucgagau cgaguucgac     3180

aagaagaucc uccccgggcu guaccagucc cgggacguca gcgcccgcaa caaguucucc     3240

augaagccca ucgacaaggc ccaguggacc agcaaggagg acgugcugau guucggcaac     3300

gugaaggccu ccggcgucuu cgagaagacc cucaagaaga acuacgugcu gcggaaguac     3360

cacguggacg uccuggggaa caaguucgag gugaaggacg agauccucaa ggacauccug     3420

gaguga                                                                3426


<210>  1689
<211>  3429
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1689
auguacagcg ugggccugga caucgggauc gccuccugcg gcuacagcau caucaacgac       60

gucaacggga acaucgugga gcucggcacc cugcuguuca agucccgcaa cagcgacaac      120

aacgaggagc ggcgcaccaa ccgggggucc cgccggcuca ucaaccgccg gcgcacgcgg      180

cuggacgacg gcaagaagcu gcucgccgag aagggcuuca agcaggacag caagcugaag      240

cacgugugcc ccuacgagcu gcgcgucaag gggcuccacg agaagcugac ccagggcgag      300

aucuacaagg uggugcugca cauccucaag aagcggggga ucuccuaccu ggacgagagc      360

gacgagaacu ccagcggcau cgacggggcg uccuacaagg accaggucaa ccugaaccuc      420

aaccugcaga aggagcugac cccgggccag auccagcuca agcgccugaa ggagaacggc      480

cggaucaaga gcggggugac cuucgagaac ggcuaccagc ugaacguguu cuccgucagc      540

gccuacgcca acgagcucaa caagauccug acgaagcagu ccgaguacua cagcgagauc      600

gaccagaucu ggaucgaccg cuucaccaag aacggguacc ggaaggaggc cggccuggug      660

uaccgcaagc ggcccuacua cgacggcccc gggaacgaga agaacaacuc caucuacggc      720

cgcugggcca acuucaagga ggacgggcac cccaaggaga acaucuucga ccagcucauc      780

ggccuggaca gcguggacuc cacccagguc cgggcgagcg ccagcacccu gacggcccag      840

aaguacaacc uccugaacga ccugaacaac cucaccauca ucgaggacga ccgcaaguuc      900

accaccgacg agaagguguc caucaucaac uaccugauga cgcaggacau cacccgguuc      960

gggccgaagg agcuggccaa guucuaccag cucgaggugg accagauccg cggcuggcgc     1020

aucgacaaca agggcaaccc cgagauccac agccugaagg ucuaccggga cuggaagaag     1080

aucuucaaca ccuccagcau cgaccugacc uccauccaga cguggguggu ggacgagcuc     1140

gcgaacguca ucacccugaa caccgagauc cccagcaccc uggccacgcu caagaucaag     1200

auccccgaca ucgaccagca gauccugaac uucguggaga acaacuucua cgagcugaag     1260

aagaagaccu ccaagaacag cuggcacucc uucagccuca agacccugaa cauccugauc     1320

ccggagcucc ugaacaccuc cgaggagcag aacacgaucc uggagcgccu caaccugaag     1380

uucgaccuga agaacaagua cgccgagaag aaccagaucc ccgugaagga gcugcucgag     1440

cagaucuaca accccaccgu caccaagagc gugcaccagu ccaucaagcu guucaacgag     1500

aucaucgaga aguacgggaa gaacaacauc agcuacguga ccaucgagau gccccgggac     1560

aagaacgacc aggaccagaa ggacacgauc aagcgcauca acaagcugaa cuccgagcgg     1620

aagaacgaca gcgagaagua cuuccucgag cgcuccggcu gggacaccau ccgguucgag     1680

agcgcccuga agaagccgcg cuucgccgag aagcuccugu ucuacuacga gcagaagggg     1740

aagugcgcgu acuccggcca ggacauccug gccgagagcc ugcucaacga gagcugcgag     1800

gucgaccaca ucaucccccu guccaucagc uucgacgacu ccauccacaa caaggugcug     1860

gucaccgcca gcuccaacca ggagaagggg cagcggaccc ccuacauggc cuucaucgac     1920

ggcgccaagc ucggccagag cugggacgac uucguggcgu gggugaacuc cugcggguuc     1980

caccgcaaga agguccagaa ccugcuguac gaggagaaca ucuucaaccc cgacgugcag     2040

cggggcuucg ugagccgcaa ccucaacgac acgcgguacg ccucccgcau cguccugaac     2100

gccauccaga gcuucuucua caagcagaag gaccugacca aggugaaggu ggucaacggg     2160

uccuucaccc acacccuccg gaagaagugg ggcagcuccc uggacaagaa ccgcgacacg     2220

uaccaccacc acgccgugga cgccacccug ugcgcgguga ccccguucau caacaucguc     2280

aaguacgagu ucuucgagga ggacgggauc aaguacaugg uggaccucac caccggcgag     2340

aagguggccu acagcgacua caagaagaug aaguucuacg acaagcgggc cuacgucccc     2400

aucugggacg gcuucaucaa gcagcugacg cccgccaacc uguaccccaa gaucaaguuc     2460

ucccaccagg uggacagcaa guggaaccgc aagaucuccg acgccaccau cuacagcacc     2520

cggaagaucg uggagaccaa gaucaacaag aagggggucg cgaaggagac ggugaaggac     2580

aucgugaucg acaagaucaa ggacaucuac accccggagg gcuggcgcaa guucaaggag     2640

aagcaggaca agcuccugau gaagaacuuc gacgagaaga ccuacaacaa gcugaucaag     2700

aucgccaagg aguaccccga gaagaaggag guccagacca gcaacgggaa ggugucccuc     2760

guggagguca gccccuucaa gauguacugc gaggagcaga acgugcccgc cauccgcaag     2820

uacuccaaga agggcaacgg cccguacauc cggagccuga aguucuacga caaggagcug     2880

gggaagcaca ucaacaucac gaaggacucc uacggcaacg acaucgagcg caccauccgg     2940

aacaagaaga ucauccucaa gaaccagaac agcuggcgca ccgacgugua cuacaacucc     3000

gucaacaacu gcuacgagcu gcuggggauc aaguacugcc accucaaguu cuaccggggc     3060

aaguacggga uccccaagag caucuacgac gagcugaaga aggaggacga gaucuccagc     3120

gaguccgugu ucaaguucag ccuguaccgc cacgaccggg ugcgcuucga gaaggacggc     3180

gaguacaucg aggcccucuu cggcuccaag aacgagagca acaagaacua ccuggagcug     3240

ucccccaucg acaagaacaa guggcucagc aaggagaacg ucaaguucuu cggggaggug     3300

gcggccuccg gccgguucau caaggggcug aagcccggca ucaaggugau caagcagagc     3360

accgacuaca uggggcacgu cuacgccauc caccaggaga agcugaaguu cgugcucucc     3420

gacgccuga                                                             3429


<210>  1690
<211>  3435
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1690
augaccagca agcaggagaa gcacggcgcc cugaccuucg ggcucgacau cggcaucgcc       60

uccguggggu gggcgguccu gggcgagagc cgcaucgugg accugggggu gcggugcuuc      120

gacaaggccg agacggccaa ggagggcgag ucccucaacc ugacccgccg gauggcccgc      180

cugcuccggc gccggcugcg ccggcgcgcc uggcggcuga ccaagcucgc gcgccugcug      240

aagcgggagg gccucaucgc cgacgccaac cuguucaagc acaagaucga gaagaacacc      300

cccgucccca agagcuccug gcagcugcgc guggacgggc ucgaccggca gcugaacgcc      360

gaggaguggg cgcgcgugau cuaccaccug ugcaagcacc ggggcuucca cuggaucagc      420

cgcgccgagg agaaggccgc cgagggggac uccaagggcg agggcgggaa ggucaagcag      480

ggccucgccg ggacgaagaa gcugaugcag gagaaguccu accggaccgc ggccgagaug      540

gugcuggccg aguuccccga cgcccagcgc aacaagcagg gcgacuacag caaggcccuc      600

agccggaucc ugcuggggga cgagcucaag gcgcuguucg agcaccagcg caagcugggc      660

aaccccgugg ccaccgagaa gcucgaggcc gccauccugg gcaacgggga caagaagugc      720

ggccuguucu gggcccagaa ccccgcgcuc gccgggaaca agcugcugga gaugcucggc      780

cgcugcaccu ucgagaagac cgggggcgac gacggcaagg gggaguaccg ggccccgaag      840

gccuccuuca gcgccgagcg ccacgucugg cugacgcacc ugaacaaccu ccggaucgug      900

guggacggca ccacccgccc ccugaacgag gcggagcggc gcgucgcccu gcccaugccc      960

uaccagcagg ccggggaccu cuccuacaag cagcugggcg gggcccugau caaggagggc     1020

uuccuccaga aggacggcuu caaguucgcg gggcugccgu accccacgaa gcaggccagc     1080

gaggagaagg ccaaggaccc cgagaccucc gugcugugca agcuccccgc cuggcacacc     1140

cugcggaaga cccuggagaa gcacggccuc gagagcgagu ggcagaagcu guccacggac     1200

gcccuggacg ggaagccgca gcugcucgac aacaucgcgu gggugcugag cgucuacaag     1260

gacgacgacg aggugaagac cgagcugggc aagcucaacc ugcccaacac cgagaagaug     1320

aucgacgccc ugcucgacau ccgcuucgac aaguucucca accugagccu gaaggcccuc     1380

agcaagaucg ugcccuucau ggagcagggg cagcgguacg acgaggccgu cgccaacauc     1440

cccgaguacg gccaccacuc ccagcugcac aaggugggcg cgggggagca caaguaccug     1500

ccgcccuucu acaaggagcg cgacaagacc ggccggaugg uguucaacga cgacauggac     1560

aucccccgca accccgucgu gcuccgggcc cugaaccagg cccgcaaggu ggucaacgcc     1620

cugaucaagg aguacgggag cccgcacgag gugcacaucg agauggcccg ggaccucucc     1680

cgcccccugg acgagcggcg cgacaucgag aagcugcaga aggaguucgg cgagcggaac     1740

aagaaggaca aggagcuguu cgcgcaggac uucaacaucg ucggcacggu gaaggggaag     1800

caguucgaga aguaccagcu cuaccgcgag cagcagggca agugcaucua ccccagcaug     1860

gacgagaagc aggggaucug cugcuacucc cugaagcccc ucgaccugca ccgcgugcug     1920

uacgaggagg gcuacgccga gaucgaccac gcccucccgu acagccgguc cuucgacgac     1980

agcaagaaca accgcguccu ggugcuggcc aaggagaacc gggacaaggg gaaccgcacc     2040

cccuucgagu accucggcga caaggacaac uccgagcccu ggcggcaguu cguggcguuc     2100

gucgagggca acaaggccua ccgccuggcc aagcggagcc gccugcuccg gaaggacuuc     2160

ggggacaagg aggccaagga guuccgcgag cggaaccuga acgacacccg cuacaucugc     2220

aaguucuuca agaacuacgu ggagcaguac cugcagcucc acgaggacuc cgaggccaag     2280

cggugcgugg uccugagcgg ccagaugacc uccuuccugc gcgcgcggug ggggcucgug     2340

aaggugcgcg gcgacagcga ccggcaccac gcccuggacg ccgccgucgu ggccgcgugc     2400

ucccacggga uggugaagcg ccugagcgac uacucccggc gcaaggagcu cgagcagguc     2460

cgggagggcu ucguggacgu ggacacgggc gagaucguca accccgccau guuccagcag     2520

guccaggagc acuucccgaa ccccuggccc cacuuccgcg acgaggugaa gcugcgccug     2580

agcaucgacg acccgacccu ccugcggucc gagauggaga aguucgggac cuacaccccc     2640

gagagccugg aggcccuccg cccccuguuc gugagccggg ccccgcagcg ccggaacucc     2700

ggcgccgcgc acaaggacac gaucuacgcc cagccgaagc gccugguggc ccagggcggg     2760

gugacccaga aggucgcccu cagcucccug acccugaagg accucgacaa gcugaucgac     2820

ccccaccgga acgagcgccu guacgcggcc auccgggccc gccucgagca gcacgggggc     2880

aagggcgaca aggccuuccc cgccagcaac ccccugcgga agccggacuc cgaggggaac     2940

cccaccggcc ccaucgugcg cggggugacg auggucaucg acaagcugag cggcaucccc     3000

auccgcgggg gcaucgcgaa gaacgacucc auggugcggg uggacgucuu caccaaggcc     3060

aacaaguucc accucgugcc gguguacguc caccacagcg ugcugggccu gcccaaccag     3120

gccaucgucg ggggcaagga ggaguccgag uggacccaga ucgacgacag cuucgacugg     3180

cacuucucca ucuaccccaa cgaccucauc cgggugaccc ugaagcagga ggggcgcuuc     3240

ggcuacuacg ccggcugcga ccggagcacg gccgcgaucc accugugggc ccacgaccgc     3300

aaccagcagg ucgggaagga cggccucauc cgguccaucg gggugaagac cgcccugagc     3360

aucgagaagu ucaacgugga cgugcugggc aaccgcuacc ccgccccgcc cgagaagcgc     3420

cgggaccucg ccuga                                                      3435


<210>  1691
<211>  3435
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1691
augaccagca agcaggagaa ccugggcgcc cucaccuucg ggcuggacau cggcaucgcc       60

uccguggggu gggcgguccu gggcgagaac cgcaucaucg accucggggu gcggugcuuc      120

gacaaggccg agacggccaa ggagggcgag agccugaacc ugacccgccg gauggcccgc      180

cuccugcggc gccggcugcg ccggcgcgcc uggcggcuca ccaagcuggc gcgccugcuc      240

aagcgggagg gccugaucgc cgacgccaac cuguucaagc acaagaucga gaagaacacc      300

cccgugccga aguccagcug gcagcuccgc gucgacgggc uggaccggca gcugaacgcc      360

gaggaguggg cgcgcgugau cuaccaccuc ugcaagcacc ggggcuucca cuggaucucc      420

cgcgccgagg agaaggccgc cgagggggac agcaagggcg agggcgggaa ggugaagcag      480

ggccuggccg ggacgaagaa gcugaugcag gagaaguccu accggaccgc ggccgagaug      540

guccucgccg aguuccccga cgcccagcgc aacaagcagg gcgacuacag caaggcccug      600

ucccggaucc ugcucgggga cgagcugaag gcgcuguucg agcaccagcg caagcucggc      660

aaccccgugg ccaccgagaa gcuggaggcc gccauccugg gcaacgggga caagaagugc      720

ggccucuucu gggcccagaa ccccgcgcug gccgggaaca agcugcucga gaugcugggc      780

cgcugcaccu ucgagaagac cgggggcgac gacggcaagg gggaguaccg ggccccgaag      840

gccagcuucu ccgccgagcg ccacgugugg cugacgcacc ucaacaaccu gcggaucguc      900

guggacggca ccacccgccc ccugaacgag gcggagcggc gcguggcccu ccccaugccc      960

uaccagcagg ccggggaccu gagcuacaag cagcugggcg gggcccucau caaggagggc     1020

uuccugcaga aggacggcuu caaguucgcg gggcugccgu accccacgaa gcaggccucc     1080

gaggagaagg ccaaggaccc cgagaccagc guccucugca agcugcccgc cuggcacacc     1140

cugcggaaga cccucgagaa gcacggccug gaguccgagu ggcagaagcu gagcacggac     1200

gcccuggacg ggaagccgca gcuccuggac aacaucgcgu gggugcuguc cguguacaag     1260

gacgacgacg aggucaagac cgagcucggc aagcugaacc ugcccaacac cgagaagaug     1320

aucgacgccc uccuggacau ccgcuucgac aaguucagca accugucccu caaggcccug     1380

agcaagaucg ugcccuucau ggagcagggg cagcgguacg acgaggccgu ggccaacauc     1440

cccgaguacg gccaccacag ccagcugcac aaggucggcg cgggggagca caaguaccuc     1500

ccgcccuucu acaaggagcg cgacaagacc ggccggaugg uguucaacga cgacauggac     1560

aucccccgca accccguggu ccugcgggcc cugaaccagg cccgcaaggu ggugaacgcc     1620

cucaucaagg aguacggguc cccgcacgag guccacaucg agauggcccg ggaccugagc     1680

cgcccccugg acgagcggcg cgacaucgag aagcuccaga aggaguucgg cgagcggaac     1740

aagaaggaca aggagcuguu cgcgcaggac uucaacaucg ugggcacggu caaggggaag     1800

caguucgaga aguaccagcu guaccgcgag cagcagggca agugcaucua ccccuccaug     1860

gacgagaagc aggggaucug cugcuacagc cucaagcccc uggaccugca ccgcgugcuc     1920

uacgaggagg gcuacgccga gaucgaccac gcccugccgu acucccggag cuucgacgac     1980

uccaagaaca accgcgugcu gguccucgcc aaggagaacc gggacaaggg gaaccgcacc     2040

cccuucgagu accugggcga caaggacaac agcgagcccu ggcggcaguu cguggcguuc     2100

guggagggca acaaggccua ccgccuggcc aagcgguccc gccuccugcg gaaggacuuc     2160

ggggacaagg aggccaagga guuccgcgag cggaaccuga acgacacccg cuacaucugc     2220

aaguucuuca agaacuacgu cgagcaguac cuccagcugc acgaggacag cgaggccaag     2280

cggugcgugg ugcuguccgg ccagaugacc agcuuccucc gcgcgcggug ggggcugguc     2340

aaggugcgcg gcgacuccga ccggcaccac gcccuggacg ccgccguggu cgccgcgugc     2400

agccacggga uggugaagcg ccucuccgac uacagccggc gcaaggagcu ggagcagguc     2460

cgggagggcu ucguggacgu ggacacgggc gagaucguga accccgccau guuccagcag     2520

guccaggagc acuucccgaa ccccuggccc cacuuccgcg acgaggugaa gcugcgccuc     2580

uccaucgacg acccgacccu gcugcggagc gagauggaga aguucgggac cuacaccccc     2640

gagucccucg aggcccugcg cccccuguuc gugagccggg cgccccagcg ccggaacagc     2700

ggcgccgccc acaaggacac gaucuacgcc cagccgaagc gccucgucgc ccagggcggg     2760

gugacccaga agguggcccu guccagccug acccucaagg accuggacaa gcugaucgac     2820

ccccaccgga acgagcgccu cuacgcggcc auccgggccc gccuggagca gcacgggggc     2880

aagggcgaca aggccuuccc cgccuccaac ccccugcgga agccggacag cgaggggaac     2940

cccaccggcc ccaucguccg cggggugacg auggugaucg acaagcucuc cggcaucccc     3000

auccgcgggg gcaucgcgaa gaacgacagc augguccggg uggacguguu caccaaggcc     3060

aacaaguucc accugguccc gguguacgug caccacuccg uccugggccu ccccaaccag     3120

gccaucgugg gcgggaagga ggagagcgag uggacccaga ucgacgacuc cuucgacugg     3180

cacuucagca ucuaccccaa cgaccugauc cgggugaccc ugaagcagga ggggcgcuuc     3240

ggcuacuacg ccggcugcga ccgguccacg gccgcgaucc accucugggc ccacgaccgc     3300

aaccagcagg ucgggaagga cggccugauc cggagcaucg gggugaagac cgcccugucc     3360

aucgagaagu ucaacgugga cguccucggc aaccgcuacc ccgccccgcc cgagaagcgc     3420

cgggaccugg ccuga                                                      3435


<210>  1692
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1692
augaagacca uccuggggcu cgaccugggg accaacagca ucggcugggc ccuggugaag       60

gagacggaga acuccaacga gaaguccgag aucaucaagc ucgggguccg cgugaacccc      120

cugaccgugg acgagaagac caacuucgag gccggccggc cgcugagcac caacgcggac      180

cgcacggcca agcggagcgc ccgccggaac cuccagcgcu acaagcugcg gcgcaagaac      240

cugaucgacc uccugaucaa gcaccggcug aucgacaagg acacgccccu caccgagauc      300

gggaagaaca ccacccacca gacccuggag cugcgcgcca aggccgcgcg ggagcgcauc      360

gagcucgagg accuggcccg ggucuuccug gccaucaaca agaagcgcgg cuaccggucc      420

agccgcaagg ugaacaacga ggaggagggc cagguggucg acgggauggc cguggcgaag      480

aagcucuacg acgagaaccu gacccccggc caguacgccu acgagcugcu cuccaagggg      540

aagaaguacg ugcccgacuu cuaccggagc gaccugcagg ccgaguucga cuccaucugg      600

gaguaccaga agcaguucua cgccgacauc cuggacgacg agcucuacaa cgcccugaag      660

ggccaggggc agcagaacag ccgcaagcgg uuccuggcga ucaagggcgu cuacaccgcc      720

gagaacaagg gcaagcgcga cgaggugaag cuccagcacu acaaguggcg guccgaggcc      780

aucacgcaga agcugagcau cgaggaggug gccuacgucc ugguggagau caacaacgac      840

cucaacaagu ccagcgggua ccugggcgcc aucuccgacc gcagcaagga gcuguacuuc      900

aacaaggaga ccguggggga gaaccucugg aagcagaucc agaagaaccc gcacaccucc      960

cugaagaacc aggucuucua ccgccaggac uaccuggacg aguucgagca gaucugggag     1020

acccaggcgc aguuccaccc ccagcucacg cuggcccuga aggagcagau ccgggacgug     1080

gugaucuucu accagcgcaa gcucaagagc cagaagggcc ugcuguccuu cugccaguuc     1140

gagagcuggg agaucgagcg gaaggacgag aacggcaacg ucauccucaa caagaccacc     1200

cagcugccca agcgccagac cguggggcgg cgcguggccc ccaagagcuc cccgcuguuc     1260

caggaguuca agaucuggca gaacaucaac aaccucgaga ucgccaagau cagcgacggc     1320

aacuccaaga acaagaagga gacggaggcc cugagcgacg acgagcggaa gcuccuguuc     1380

gaggagcuga accugcgcgg gaaccugucc cagaaggagg uccuccagau ccugggccug     1440

aaggacaagg aguacaagac caacuucccc gaggggcucg agggcaaccg gaccaacgcg     1500

gcccuguuca acaucuacca gcagaucgcc gagaacgagg gcuacgggga cuggaccaag     1560

aaguccgccc aggagaucaa ggaggagcug aaggccgugu ucccccagau cggcauccag     1620

gcggacaucc ucgacuucaa cgccgagcug gacgggaagg aguucgagaa ccaggccagc     1680

uaccagcugu ggcaccuccu guacagcgcc gaggaggacg acaagaucaa cgaggaggac     1740

cagaucaucu acggcaacuc cgcggugagc cugaagaaga agcucugcga gaaguucggg     1800

uucacgcccg aguacgccaa guggaucgcc aacgucuccc ugcaggacga cuacggcaac     1860

cugagcacca aggccaugcg caagaucauc ccguaccuca ucgacggcaa cgacuacucc     1920

gaggccugcg cgcuggccgg guacaaccac agcaacuccc ugaccaagga ggagaacgac     1980

aaccgggagc gccucaacaa gcuggagcug cuccccaaga acagccugcg gaaccccgug     2040

gucgagaaga uccugaacca gauggugaac guggucaacc aggugaucga gaccuacggc     2100

aagcccgacg aggugcgcau cgagcucgcc cgggagcuga agaaguccgc cgaggagcgc     2160

gccgagauga cgaaggggau caacgaggcg acccggcgca acgaggacau caagaagcug     2220

aucaccaaga acuucggcau cccgaacccc accaagaacg acgucgugcg guaccgccuc     2280

ugggaggagc uggcgccccu ggccuacaag gacguguuca cggggcgcca gaucaagaag     2340

gaggaccucu ucagcuccaa gaucgacauc gagcacauca uccccaaggc ccugcuguuc     2400

gacgacagcu ucagcaacaa gacccucgcc uuccgggaga ccaaccugaa gaaggccgac     2460

cgcaccgccu acggcuucau cgaguccgac uacaacgcca cgcuggacga cuacauccag     2520

cgggucgaga cccucuacaa caacgccaag ggcaccauca gcaaggggaa gcgcaacaag     2580

cugcugaugg cgcagaagaa ccucccggac ggcuucaucg agcgggaccu gcgcaacucc     2640

caguacaucg ccaagaaggc caagagcaug aucgagaagg uguucaacga cgugaacguc     2700

accuccggga gcaucaccga ccggcugcgc gaggacuggg accucaucaa cgugaugaag     2760

gagcugaacu uccccaagua caaggcccug ggccucacgg agaucgagga gcgguacgac     2820

aucggccagg agaagaccaa gaagguggag gucaucaccg acuggaccaa gcgcaacgac     2880

caccggcacc acgccaugga cgcgcugacg guggccuuca ccaccaaguc ccacgugcag     2940

uaccugaaca accucaacac cauccacagc cugaaggggg acgaggucga ccuggagcuc     3000

uccaagcugu acggccugaa gaacacgauc accgaggugg ugaacaagaa gcgcaaguuc     3060

aucccgccca ugcccaacuu ccgggaggag gccaagaagc acaucgagac cauccucguc     3120

agcaucaaga acaagaacaa gguguacacc aagaacauca acaagacgaa gaaggagucc     3180

ggguaccaca ccaaggagca gcugacgccc cgcggccagc ugcacaagga gaccguguac     3240

ggcaagagca agcggcccau gaacaagccc accaagauca acaagaacuu cucccucgag     3300

caggcccagc ugaucaucaa cguccaggag aaggagcugg ugcuccgcca ccuggcccag     3360

uucgacaaca acccggcggu ggccuucgac accaagaccc ugaagaagug ccacuaccuc     3420

aagaugguca accuguga                                                   3438


<210>  1693
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1693
augagcggca acacccacgg gccccugacc uucggcuucg acaucgggau cgccuccgug       60

ggcuggugcg uccucgggga gaaccgcauc gugaaccugg gcgugcggug cuucgacaag      120

gccgagacgg cgaaggaggg cgagagccug aaccucgccc gccggaccgc ccgccugaug      180

cggcgccggc ugcgccggcg cgccuggcgg cucaccaagc uggcccgccu gcucaagcgg      240

gaggggcuga ucgcggaggc cucccgcauc cacaucccca gccccgucuc ccccuggcgg      300

cugcgcgugg agggcaucga ccggcgccuc accgacgacg agugggcccg ggugaucuac      360

caccugugca agcaccgcgg guuccacugg aucagccggg ccgaggagcg caaggcggag      420

uccgacgcca aggagggcgg gcgcgucaag aagggccuga gcgacacgca gcggcgcaug      480

accgagaagg gcuaccgguc cgccgccgag auggugcucg ccgaguuccc cgacgcgcag      540

cgcaacaagc agggggacua cggcaaggcc cugagccgga uccugcucuc cgacgagcug      600

gcccuccucu uccgccggca gcgcgagcug gggaacccga acgccggcaa cgagcuggag      660

gccaccaucc ucggcaacgg gaaccgcaag agcggccugu ucugggagca gaagccgccc      720

cuggcggggg ccgaccuccu gaagaugcug ggccggugca ccuucgagaa gaacgaguac      780

cgggccccca aggccuccuu cacggccgag cgccacgugu ggcucacccg gcugaacaac      840

cugcgcgucg ugguggacgg gaccacccgg agccucgacg acgcggagca ccgccugguc      900

cugccgcucc ccuacaagca ggccggcgac uucacguaca agcagcugcg gaccgcccug      960

accaaggccg gccuccugcc cgagggguuc aaguucgccg gccuguccua ccccaccggg     1020

cgccagcucg acgacccgaa ggcgaaggac cccgagagcg agaagcuggu gcggcugccc     1080

gccuggcagg agcuccgcaa gacgcugaag gacaagaacc uggagaccga gugggagggc     1140

auggccgggg ccgcgaccgg cggcaacccc gagcuccugg acgagaucgc ccgcgugcug     1200

uccgucuuca aggacgggga cgaggugacc gccgagcugu ggaagcuccc gcugcccggc     1260

gggaaggcca ugaucgacgc ccugagcgag aucuccuucg acaaguucca cgcgcucagc     1320

cugaaggccc ugcggaagau cgugccccac auggagagcg gccuccgcua cgacgaggcc     1380

ugcgccgucg ccggguacca ccacucccag cugcacgcgg ccggcgccgg cgagcacaag     1440

uuccugcccc cguucuacag ccggcgcgac aaggacgggc ggaugguguu caacgaggag     1500

cucgacgugc cccgcaaccc cgucgugcug cgggcccuga accaggcccg caaggugcuc     1560

aacgcgcugg uccggacgua cggcuccccc accgccgugc acaucgagau ggcccgcgac     1620

cugagccggc cgcucgacga gcgcaacaag gucaagaagg cccaggacga guaccgggag     1680

cgcaacgaga aggacaaggc ggccuucgcc gccgaguacg ggaucgcccc caagucccgg     1740

gaguucgaga aguuccagcu guaccgcgag cagcagggca agugcgcgua cagccugaag     1800

ucccucgacc ugcaccgggu gcugcacgac aucggcuacg ccgaggugga ccacgcccuc     1860

cccuacagcc gcuccuacga cgacagcaag aacaacaagg uccuggcccu gaccgaggag     1920

aaccggaaca aggggaaccg caccgccuac gaguaccuca cguccuuccc cggcggggag     1980

aacggcgagc gguggcgcaa cuucagcgug gcgguggaga ccaacaaggc cuaccgccug     2040

gccaagcgga cccgccugcu ccggaaggac uucuccgggc gcgccgccga ggaguuccgg     2100

gagcgcaacc ugaacgacac ccgguacauc ugccgcuucu ucaagaacac ggucgagcgg     2160

uuccugaagc ucgacgcgaa ggugggcgcc ggcgacaccg ccgagaucag cgccaagcgc     2220

ugcguggucc uguccgggca gaccaccgcg uuccugcggg cccgcugggg ccuccugaag     2280

gugcggggga acagcgaccg ccaccacgcc cuggacgccg ccguggucgc ggccugcucc     2340

cacggcaugg ugaagcggcu cggggacuac agccgcacga aggagcuggc caaggugcac     2400

gacggcuucc cggaccccga gaccggcgag aucaucgacc ccgucauguu ccagcagcug     2460

cacgcccacu ucccggaccc cuggccccac uuccgggccg agcucgaggc gcgccugaac     2520

guggacgaca ccgccgaccu gcgggcccgc auggagcugu ucgggaccua cgcccccgag     2580

gcccuggaga cgcugcgccc ccucuucgug ucccgggcgc cccagcgccg gaacagcggc     2640

gccgcccaca aggacaccau cuacgcccag cccgagcgcc ugaaggagca gggcgggguc     2700

acccagaagg ugccccugac cagccucacc cugaaggacc uggacaagcu caucgacccg     2760

caccggaacg agaagcugua cgccgcgauc cgcgcccggc uggaggccca cgggggcaag     2820

gccgacaagg cguuccccgc cggcaacccc cuccgcaagc ccgaccggga ggggaacccg     2880

acgggccccg ugguccgcac cgugaccaug gugaucgaca agcuguccgg gauccccguc     2940

cggggcggga ucgccaagaa cgacaccaug cugcgcgugg acaucuuccg gcacaagaag     3000

gacggcaagu uccaccucgu gcccgucuac guguaccacg ccguggccaa ggaguucccg     3060

aaccgcgcga ucguccaggc caaggacgag gacgagugga cgcugaucga cgacaaguuc     3120

gacuucugcu ucagcgccca ccccaacgac cugcucaaga ucacccagaa guccggcgag     3180

accaucuucg gguacuaccg gggcugccac agcgggaccg ccgccaucaa caucgcgcug     3240

cacgaccgcu acgccuacgc cgagaagacg accucccugg ccuucagcaa gaagaacccc     3300

ggcaagccca ucccgaacga caagcucggc cugaucgagg ggaucggcgu gaagauggcc     3360

gcgaacaugg agaaguucca cguggacacc cuggggaaca ucuaccccgc cccgcccgag     3420

cagcggcgcg gccucgccug a                                               3441


<210>  1694
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1694
auggccuacc gccugggccu cgacaucggg aucaccagcg ugggcugggc cgucguggcg       60

cuggagaagg acgaguccgg gcugaagccc gugcggaucc aggaccucgg cguccgcauc      120

uucgacaagg ccgaggacag caagaccggg gccucccugg cccugccgcg gcgcgaggcc      180

cggagcgcgc gccggcgcac gcggcgccgg cgccaccggc ucuggcgcgu gaagcggcug      240

cuggagcagc acggcauccu cuccauggag cagaucgagg cccuguacgc ccagcgcacc      300

agcucccccg acguguacgc ccugcggguc gcgggccucg accgcugccu gaucgccgag      360

gagaucgccc gggugcugau ccacaucgcc caccgccggg gguuccagag caaccgcaag      420

uccgagauca aggacagcga cgccggcaag cuccugaagg cggugcagga gaacgagaac      480

cugaugcagu ccaaggggua ccgcaccguc gccgagaugc ucgugagcga ggccaccaag      540

acggacgccg agggcaagcu ggugcacggg aagaagcacg gcuacgucuc caacgugcgg      600

aacaaggccg gcgaguaccg ccacaccgug agccggcagg cgaucgucga cgaggugcgc      660

aagaucuucg ccgcccagcg ggcccugggg aacgacguga uguccgagga gcucgaggac      720

agcuaccuga agauccugug cucccagcgc aacuucgacg acggccccgg gggcgacagc      780

cccuacggcc acggguccgu cagcccggac ggcgugcggc agagcaucua cgagcgcaug      840

gugggguccu gcaccuucga gaccggcgag aagcgggcgc cccgcagcuc cuacagcuuc      900

gagcgguucc agcuccugac gaaggucgug aaccugcgca ucuaccggca gcaggaggac      960

gggggccgcu accccugcga gcucacccag accgagcggg cccgcgugau cgacugcgcc     1020

uacgagcaga ccaagaucac guacggcaag cugcggaagc ugcucgacau gaaggacacc     1080

gaguccuucg ccgggcugac cuacggccug aaccgcagcc ggaacaagac cgaggacacg     1140

gucuucgugg agaugaaguu cuaccacgag guccgcaagg cccuccagcg cgccggggug     1200

uucauccagg accuguccau cgagacccug gaccagaucg gcuggauccu cagcgugugg     1260

aaguccgacg acaaccggcg caagaagcug agcacccugg ggcucuccga caacgucauc     1320

gaggagcugc ugccccucaa cggcagcaag uucggccacc ugucccugaa ggcgauccgg     1380

aagauccucc cguuccugga ggacggguac agcuacgacg uggccugcga gcuggccggc     1440

uaccaguucc aggggaagac cgaguacgug aagcagcgcc uccugccgcc ccugggcgag     1500

ggggagguca cgaaccccgu ggugcggcgc gcccuguccc aggcgaucaa ggucgugaac     1560

gccgugaucc ggaagcacgg cagccccgag uccauccaca ucgagcucgc ccgcgagcug     1620

agcaagaacc uggacgagcg gcgcaagauc gagaaggccc agaaggagaa ccagaagaac     1680

aacgagcaga ucaaggacga gauccgggag auccucggcu ccgcccacgu caccgggcgc     1740

gacaucguga aguacaagcu guucaagcag cagcaggagu ucugcaugua cagcggcgag     1800

aagcuggacg ugacccggcu guucgagccc ggguacgcgg aggucgacca caucaucccc     1860

uacggcauca gcuucgacga cuccuacgac aacaaggugc uggugaagac cgagcagaac     1920

cgccagaagg gcaaccggac gccccuggag uaccuccgcg acaagccgga gcagaaggcc     1980

aaguucaucg cccuggucga gagcaucccc cugucccaga agaagaagaa ccaccuccug     2040

auggacaagc gggccaucga ccuggagcag gagggguucc gcgagcggaa ccucagcgac     2100

acccgcuaca ucacccgggc ccugaugaac cacauccagg cguggcuccu cuucgacgag     2160

accgccucca cgcgcagcaa gcgcguggug ugcgucaacg gcgccgugac cgccuacaug     2220

cgggcccgcu gggggcugac caaggaccgg gacgcgggcg acaagcacca cgccgccgac     2280

gccguggucg uggcgugcau cggggacucc cugauccagc gcgugaccaa guacgacaag     2340

uucaagcgga acgcccucgc cgaccgcaac cgguacgucc agcaggucag caaguccgag     2400

ggcaucacgc aguacgugga caaggagacc ggcgaggugu ucaccuggga gagcuucgac     2460

gagcgcaagu uccugcccaa cgagccccug gagccguggc ccuucuuccg ggacgagcuc     2520

cuggcccgcc uguccgacga ccccagcaag aacauccggg ccaucgggcu ccugaccuac     2580

uccgagacgg agcagaucga ccccaucuuc gugagccgca ugccgacccg gaaggugacc     2640

ggcgcggccc acaaggagac cauccgcagc ccccggaucg ucaaggugga cgacaacaag     2700

gggaccgaga uccagguggu cguguccaag guggcccuga cggagcucaa gcugaccaag     2760

gacggcgaga ucaaggacua cuuccgcccc gaggacgacc cccggcugua caacacccuc     2820

cgcgagcggc ugguccaguu cgggggcgac gccaaggccg cguucaagga gccgguguac     2880

aagaucucca aggacggcag cgugcgcacc cccguccgca aggugaagau ccaggagaag     2940

cugacgcucg gggugcccgu ccacggcggg cggggcaucg ccgagaacgg gggcauggug     3000

cgcaucgacg uguucgccaa gggcgggaag uacuacuucg ucccgaucua cguggccgac     3060

gugcugaagc gggagcugcc caaccgccuc gccaccgcgc acaagcccua cagcgagugg     3120

cgggucgugg acgacuccua ccaguucaag uucagccugu accccaacga cgccgucaug     3180

aucaagccgu cccgcgaggu ggacaucacc uacaaggacc ggaaggagcc cgugggcugc     3240

cgcaucaugu acuucgucag cgccaacauc gccuccgcga gcaucucccu gcggacccac     3300

gacaacagcg gggagcucga gggccugggg auccagggcc uggagguguu cgagaaguac     3360

guggucggcc cccucgggga cacgcacccc guguacaagg agcgccggau gccguuccgc     3420

guggagcgga agaugaacug a                                               3441


<210>  1695
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1695
auggccuacc gccugggccu cgacaucggg aucaccagcg ugggcugggc cgucguggcg       60

cuggagaagg acgaguccgg gcugaagccc gugcggaucc aggaccucgg cguccgcauc      120

uucgacaagg ccgaggacag caagaccggg gccucccugg cccugccgcg gcgcgaggcc      180

cggagcgcgc gccggcgcac gcggcgccgg cgccaccggc ucuggcgcgu gaagcggcug      240

cuggagcagc acggcauccu cuccauggag cagaucgagg cccuguacgc ccagcgcacc      300

agcucccccg acguguacgc ccugcggguc gcgggccucg accgcugccu gaucgccgag      360

gagaucgccc gggugcugau ccacaucgcc caccgccggg gguuccagag caaccgcaag      420

uccgagauca aggacagcga cgccggcaag cuccugaagg cggugcagga gaacgagaac      480

cugaugcagu ccaaggggua ccgcaccguc gccgagaugc ucgugagcga ggccaccaag      540

acggacgccg agggcaagcu ggugcacggg aagaagcacg gcuacgucuc caacgugcgg      600

aacaaggccg gcgaguaccg ccacaccgug agccggcagg cgaucgucga cgaggugcgc      660

aagaucuucg ccgcccagcg ggcccugggg aacgacguga uguccgagga gcucgaggac      720

agcuaccuga agauccugug cucccagcgc aacuucgacg acggccccgg gggcgacagc      780

cccuacggcc acggguccgu cagcccggac ggcgugcggc agagcaucua cgagcgcaug      840

gugggguccu gcaccuucga gaccggcgag aagcgggcgc cccgcagcuc cuacagcuuc      900

gagcgguucc agcuccugac gaaggucgug aaccugcgca ucuaccggca gcaggaggac      960

gggggccgcu accccugcga gcucacccag accgagcggg cccgcgugau cgacugcgcc     1020

uacgagcaga ccaagaucac guacggcaag cugcggaagc ugcucgacau gaaggacacc     1080

gaguccuucg ccgggcugac cuacggccug aaccgcagcc ggaacaagac cgaggacacg     1140

gucuucgugg agaugaaguu cuaccacgag guccgcaagg cccuccagcg cgccggggug     1200

uucauccagg accuguccau cgagacccug gaccagaucg gcuggauccu cagcgugugg     1260

aaguccgacg acaaccggcg caagaagcug agcacccugg ggcucuccga caacgucauc     1320

gaggagcugc ugccccucaa cggcagcaag uucggccacc ugucccugaa ggcgauccgg     1380

aagauccucc cguuccugga ggacggguac agcuacgacg uggccugcga gcuggccggc     1440

uaccaguucc aggggaagac cgaguacgug aagcagcgcc uccugccgcc ccugggcgag     1500

ggggagguca cgaaccccgu ggugcggcgc gcccuguccc aggcgaucaa ggucgugaac     1560

gccgugaucc ggaagcacgg cagccccgag uccauccaca ucgagcucgc ccgcgagcug     1620

agcaagaacc uggacgagcg gcgcaagauc gagaaggccc agaaggagaa ccagaagaac     1680

aacgagcaga ucaaggacga gauccgggag auccucggcu ccgcccacgu caccgggcgc     1740

gacaucguga aguacaagcu guucaagcag cagcaggagu ucugcaugua cagcggcgag     1800

aagcuggacg ugacccggcu guucgagccc ggguacgcgg aggucgacca caucaucccc     1860

uacggcauca gcuucgacga cuccuacgac aacaaggugc uggugaagac cgagcagaac     1920

cgccagaagg gcaaccggac gccccuggag uaccuccgcg acaagccgga gcagaaggcc     1980

aaguucaucg cccuggucga gagcaucccc cugucccaga agaagaagaa ccaccuccug     2040

auggacaagc gggccaucga ccuggagcag gagggguucc gcgagcggaa ccucagcgac     2100

acccgcuaca ucacccgggc ccugaugaac cacauccagg cguggcuccu cuucgacgag     2160

accgccucca cgcgcagcaa gcgcguggug ugcgucaacg gcgccgugac cgccuacaug     2220

cgggcccgcu gggggcugac caaggaccgg aacgcgggcg acaagcacca cgccgccgac     2280

gccguggucg uggcgugcau cggggacucc cugauccagc gcgugaccaa guacgacaag     2340

uucaagcgga acgcccucgc cgaccgcaac cgguacgucc agcaggucag caaguccgag     2400

ggcaucacgc aguacgugga caaggagacc ggcgaggugu ucaccuggga gagcuucgac     2460

gagcgcaagu uccugcccaa cgagccccug gagccguggc ccuucuuccg ggacgagcuc     2520

cuggcccgcc uguccgacga ccccagcaag aacauccggg ccaucgggcu ccugaccuac     2580

uccgagacgg agcagaucga ccccaucuuc gugagccgca ugccgacccg gaaggugacc     2640

ggcgcggccc acaaggagac cauccgcagc ccccggaucg ucaaggugga cgacaacaag     2700

gggaccgaga uccagguggu cguguccaag guggcccuga cggagcucaa gcugaccaag     2760

gacggcgaga ucaaggacua cuuccgcccc gaggacgacc cccggcugua caacacccuc     2820

cgcgagcggc ugguccaguu cgggggcgac gccaaggccg cguucaagga gccgguguac     2880

aagaucucca aggacggcag cgugcgcacc cccguccgca aggugaagau ccaggagaag     2940

cugacgcucg gggugcccgu ccacggcggg cggggcaucg ccgagaacgg gggcauggug     3000

cgcaucgacg uguucgccaa ggacggcaag uacuacuucg ucccgaucua cguggccgac     3060

gugcugaagc gggagcugcc caaccgccuc gccaccgcgc acaagcccua cagcgagugg     3120

cgggucgugg acgacuccua ccaguucaag uucagccugu accccaacga cgccgucaug     3180

aucaagccgu cccgcgaggu ggacaucacc uacaaggacc ggaaggagcc cguggggcug     3240

cgccucaugu acuucgccgg cgccgacauc agcggggcga cccugacgcu gcggacccac     3300

gacaacuccg gcgagcucaa ggggcugggc auccagggcc uggaggucuu cgagaaguac     3360

gugguggggc cccucggcga cacccacccc gucuacaagg agcgccggau gccguuccgc     3420

guggagcgga agaugaacug a                                               3441


<210>  1696
<211>  3447
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1696
augaccaagc ugaaccagcc cuacggcauc gggcucgaca ucggcagcaa cuccaucggg       60

uucgccgugg ucgacgccaa cagccaccuc cugcgccuca agggcgagac cgcgaucggg      120

gcccggcugu uccgcgaggg ccaguccgcc gccgaccggc gcggcagccg gacgacccgc      180

cggcgccugu cccggacccg cuggcggcuc agcuuccugc gcgacuucuu cgccccgcac      240

aucaccaaga ucgaccccga cuucuuccug cggcagaagu acuccgagau cagccccaag      300

gacaaggacc gcuucaagua cgagaagcgg cuguucaacg accgcacgga cgcggaguuc      360

uacgaggccu accccuccau guaccaccuc cggcugcgcc ugaugaccga cgccagccag      420

gccgacccgc gggagaucuu ccuggcgauc caccacaucc ugaagucccg cgggcacuuc      480

cucacccccg gcgccgccaa ggacuucaac accgacaagg uggaccugga ggacaucuuc      540

cccgcccuga cggaggccua cgcccaggug uaccccgacc ucgagcugac cuucgaccug      600

gccaaggccg acgacuucaa ggccaagcuc cuggacgagc aggccacccc gagcgacacc      660

cagaaggcgc uggucaaccu ccugcugucc agcgacgggg agaaggagau cgugaagaag      720

cggaagcagg uccucacgga guucgccaag gccaucaccg gccugaagac caaguucaac      780

cuggcccucg ggaccgaggu ggacgaggcc gacgcgucca acuggcaguu cagcaugggc      840

cagcuggacg acaagugguc caacaucgag acgagcauga ccgaccaggg caccgagauc      900

uucgagcaga uccaggagcu guaccgcgcc cgccuccuga acgggaucgu gcccgccggc      960

augucccuga gccaggccaa gguggccgac uacgggcagc acaacgagga ccucgagcug     1020

uucaagaccu accugaagaa gcucaacgac cacgagcugg cgaagacgau ccgggucauc     1080

uacgaccgcu acaucaacgg cgacgacgcc aagcccuucc ugcgggagga cuucgugaag     1140

gcccucacca aggaggugac cgcccacccc aacgagguca gcgagcagcu gcugaaccgc     1200

augggccagg cgaacuucau gcucaagcag cggaccaagg ccaacggggc caucccggug     1260

cagcugcagc agcgcgagcu ggaccagauc aucgccaacc aguccaagua cuacgacugg     1320

cucgccaagc ccaaccccgu ggagaagcac cgggcgagca agcccuacca gcuggacgag     1380

cugcugacgu uccgcauccc guacuacguc ggcccccuca ucacccccaa ggagcaggcc     1440

aaguccgggg agaacguguu cgccuggaug gugcggaagg accccagcgg caacaucacc     1500

ccguacaacu ucgacgagaa ggucgaccgc gaggccuccg ccaacguguu cauccagcgg     1560

augaagacca cggacaccua ccugaucggg gaggacgugc ugcccaagca gagccuccug     1620

uaccagaagu acgagguccu gaacgagcuc aacaacgugc gcgugaacaa cgaggcgcug     1680

ggcaccgacc agaagcagcg gcugauccgc gaggucuucg agcggcacuc cagcgugacc     1740

aucaagcagg ucgccgacaa ccucguggcc cacggcgacu ucgcccagcg ccccgagauc     1800

cgggggcugg ccgacgagaa gcgcuuccug uccagccucu ccacguacca ccagcugaag     1860

gagauccugc acggcgcgau cgacgacagc accaagcucc acgacaucga gaacaucauc     1920

accuggucca ccguguucga ggacgccgcc aucuucaaga cgaagcuggc cgagaucgac     1980

uggcuggacg cggccaccau caagaagcuc agcaacaucc gguaccgcgg guggggccag     2040

uucucccgca agcugcugga cgggcucaag cugggcaacg gccagaccgu cauccaggag     2100

cugaugcuca gcaaccacaa ccugaugcag auccugaccg acgagacccu caagacgacc     2160

auggccgagc ugaaccagga caagcugaag gccgacgaca ucgaggacgu gaucaacgac     2220

gccuacaccu cccccagcaa caagaaggcg cuccgccagg ugcugcgggu cguggaggac     2280

aucaagcacg ccgccaacgg gcaggacccg uccuggcugu acaucgagac cgccgacggc     2340

cccgggacgg ccggcaagcg gacccagagc cgccagaagc agauccagac cguguacgcg     2400

aacgccgccc aggagcucau cgacagcgcc guccgcgggg agcuggagga caagaucgcc     2460

gacaaggcgu ccuucaccga ccggcuggug cucuacuuca ugcagggcgg ccgggacauc     2520

uacacggggg cgccccugaa caucgaccag cugagccacu acgacaucga ccacauccuc     2580

ccccaguccc ugaucaagga cgacagccug gacaaccgcg ugcucgucaa cgccaccauc     2640

aaccgggaga agaacaacgu guucaccucc acccuguucg ccagcaagau ggcggccacg     2700

uggcgcaagu ggcacgaggc cggccugauc uccgggcgga agcuccgcaa ccugaugcug     2760

cggccggacg agaucgacaa guucgcccac ggcuucgugg cccgccagcu cgucgagacc     2820

cggcagauca ucaagcugac cgagcagauc gcggccgccc aguaccccaa caccaagauc     2880

aucgccguga aggccggccu gagccaccag cuccgcgagg agcuggacuu ccccaagaac     2940

cgggacguga accacuacca ccacgcguuc gacgccuucc uggccgcccg caucgggacg     3000

uaccuccuga agcgguacgc caagcuggcg ccguucuuca ccuacggcga guucgccaag     3060

gucgacguga agaaguuccg cgaguucaac uucaucgggg cccucaagca cgccaagaag     3120

aacaucgugg ccaaggacac cggcgagauc gucugggaca aggagcggga cauccaggag     3180

cuggaccgca ucuacaacuu caagcgcaug cugaucaccc acgaggugua cuucgagacg     3240

gcggaccucu ucaagcagac caucuacgcc gccaaggacu ccaaggagcg cgggggcagc     3300

aagcagcuga uccccaagaa gcggggcuac cccacccagg uguacggggg cauccacggg     3360

aagauccggc ugaccuggcg cuggcucaag cugcugcggc gcaagaagau guccaucgag     3420

cucagcgagc ugcagcugcu caaguga                                         3447


<210>  1697
<211>  3450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1697
auggagcgcu accacaucgg ccuggacauc gggaccagcu ccaucggcug ggccgugauc       60

ggggacgacu ucaagaucaa gcggaagaag ggcaagaacc ucaucggggu ccgccuguuc      120

aaggagggcg acaccgccgc ggagcggcgc ggcuuccgga cgcagcgccg gcgccugaac      180

cggcgcaagu ggcggcucaa gcugcuggag gagaucuucg accccuacau ggccgaggug      240

gacaaguacu ucuucgcccg ccucaaggag agcaaccugu ccccgaagga cagcaacaag      300

aaguaccugg ggucccuccu guuccccgac aucagcgacu ccaacuucua cgacaaguac      360

cccaccaucu accaccuccg gcgcgaccug auggagaagg acaagaaguu cgaccugcgg      420

gagaucuacc uggccaucca ccacaucgug aaguaccgcg gcaacuuccu cgagaagguc      480

cccgccaaga acuacaagaa cagcggggcg uccaucggcu uccugcugga ggaggugaac      540

gaccucuacg ggaacaucau cggcaacgag gacguggcca uccuggacaa cgacaaguuc      600

gaggacgucg agaagaucau ccugaacgac gagauccgga acaucgacaa gcagaagaac      660

gugggccgcc uccuggugaa ggacaagaag gagaagaaca ucgucaccgc cuucagcaag      720

gccaucuucg gguacaaguu caaccuggag gaccuccugc ugaucgaguc cgacgagaag      780

aacaagcuca ccuucaacga cgagaacauc gacgacaucu ucaacgagcu gagccacucc      840

cugaacgaca accagaugga ccuccugacg aagacccggg agaucuacuu caaguucaag      900

cugaacauga ucgugccgac cggcuacacc aucagcgagu ccaugaucga gaaguacgag      960

augcacaagg cgcaccucaa gauguacaag gaguucauca acacgcugaa cgccaaggac     1020

cgcaagaucc ugaagaacgc cuacagcgac uacaucaaca acgagaaggc caaggccgcg     1080

aacgcccagg agaacuucua caagaccgug aagaagacca ucaaggagaa cgacuccgac     1140

accgccaaga agaucaucgg gcucaucgac gagggcaacu ucaugcccaa gcagcggacg     1200

ggcgagaacg gggucauccc ccaccagcug caccagaucg agcuggaccg caucaucgag     1260

aaccaggcca aguacuaccc cuggcucgcc gaggagaacc cgguggagaa gaaccgcaag     1320

uucgcgaagu acaagcugga cgagcuggug accuuccggg uccccuacua cgugggcccc     1380

cucaucgaca agaccgagag caacaagaac gagaaggaga ccaaguucgc cuggauggug     1440

cgcaaggcca aggggacgau cacccccugg aacuucgaga accuggucga ccggaccgag     1500

uccgccaacc gcuucaucaa gcggaugacc agcaaggaca cguacaucau cggcgaggac     1560

gugcugccgg ccagcucccu ccuguacgag aaguacaagg ugcugaacga gcucaacaac     1620

aucaagguca acaagaagaa gcuggacgug gagcagaagc agcacgugua ccuggaccug     1680

uucaccaccc gcaagaacgu caccaaggac gaccucgcga cgagccugaa cugcgacgug     1740

gaguccauca ccgggcugac cgacaacaag aaguucaaca gcucccucag cuccuacauc     1800

gaccugaagg ccauccuggg caacaucguc gacgacuaca gcaagaacga ggaccucgag     1860

aagaucaucg aguacuccac caucuucgag gacggcaaca ucuacaagga gaagcugagc     1920

gagaucuccu ggcugacgga cgagcagauc gagaagcuca gcaacaucca cuucaagggg     1980

uggggccggc uguccaagaa gcugcucacc cagaucacca acgagaacgg ggagcgcauc     2040

aucgacaccc uguggaacac gagcaacaac uucauccagg ugaucuccga cgagagcauc     2100

caggccaagc uggccgagau caacggcgag uacgccaaca aguacaaccu cgaggacauc     2160

cuggacgagg cguacaccag cccccagaac aagaaggcca uccgccaggu gaugaagguc     2220

guggaggaca ucgagaaggc caugaagugc gagcccaccu ccaucgccau cgaguucacc     2280

cgggagaagc ggaaguccaa gcugacgaac acccgcuaca agaagaucag cgagaccuac     2340

gagaagauca ccgacgagcu caucagcgag uacgagcugg ggaagcugca guccgagcuc     2400

gacagcaagg ugaacaacau gcgggaccgc uacuaccugu acuucaugca gcugggccgg     2460

gacauguaca ccggcgagaa gaugaacauc gacgagcucc accagaagua cgacaucgac     2520

cacauccugc cccaguccuu caucaaggac gacagccuga acaaccgcgu ccucacgucc     2580

aagagcguga acaucaagga gaaguccgac aagaccgcgg ccgaccugua cgccgccaag     2640

augggggacu ucuggcggaa gcugcgcaag cagggccuca ugaccgagca gaaguacaag     2700

aaccugcuga cccggacgga cagcaucaac aaguacacca agcaguccuu caucaagcgc     2760

cagcucgugg agaccagcca ggucgugaag cuggccgcga acauccugca ggacaaguac     2820

cgcaacacca agaucaucga gauccgggcc cgccucaacu ccgaccugcg gaagaaguac     2880

gagcugauca agaaccgcga ggugaacgac uaccaccacg ccaucgacgg guaccucacg     2940

accuucgucg gccaguaccu guacaaggug uacccgaagc ugcggagcua cuucguguac     3000

gacgacuuca agaagcucga cuccaacuac cugaagcaca uggacaaguu caacuucauc     3060

uggaagcugg aggacaagaa ggccgaggac gucuacgaca aggugaacga cgaguucgug     3120

cucaacgucc ccgagaugaa ggaguacauc cgcaagaucu acaacuacaa guacaugcug     3180

gugagcaagg aggugaccac caagaacggg gccuucuacg accagacgaa guacaacgcg     3240

aagaccauca accugauccc caucaagaag gacaagccca ccaacaucua cggcggcuac     3300

aaggggaagg ucuccagcua caugaugcuc gugaagaucc agaagaagaa ggaggugauc     3360

uacaaguucg ucggcgugcc gcggcugugg accgacgagc uggaccgccu caucgacacg     3420

gacgagaaga aggcccugcu gaagaaguga                                      3450


<210>  1698
<211>  3459
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1698
augaacgccg agcacggcaa ggaggggcug cucaucaugg aggagaacuu ccaguaccgc       60

aucggccugg acaucgggau caccagcgug ggcugggccg uccugcagaa caacucccag      120

gacgagcccg ugcggaucac cgaccucggg gugcgcaucu ucgacgucgc ggagaacccg      180

aagaacggcg acgcccuggc cgcgccccgg cgcgacgccc ggacgacccg ccggcgccug      240

cggcgccggc gccaccggcu cgagcgcauc aaguuccugc ugcaggagaa cggccucauc      300

gagauggaca gcuucaugga gcgguacuac aaggggaacc ugcccgacgu guaccagcug      360

cgcuacgagg gccucgaccg gaagcugaag gacgaggagc uggcccaggu gcucauccac      420

aucgccaagc accgcggguu ccgguccacc cgcaaggccg agaccaagga gaaggagggc      480

ggggccgucc ugaaggcgac gaccgagaac cagaagauca ugcaggagaa gggcuaccgg      540

accgugggcg agaugcugua ccucgacgag gccuuccaca ccgagugccu guggaacgag      600

aagggguacg ugcugacgcc ccgcaaccgc ccggacgacu acaagcacac cauccuccgg      660

agcaugcugg ucgaggaggu gcacgccauc uucgccgccc agcgcgcgca cggcaaccag      720

aaggccaccg aggggcugga ggaggccuac guggagauca ugaccuccca gcggagcuuc      780

gacaugggcc ccggccucca gcccgacggg aagcccuccc cguacgccau ggagggcuuc      840

ggggaccgcg ucggcaagug cacguucgag aaggacgagu accgggcccc caaggcgacc      900

uacaccgccg agcuguucgu ggcccugcag aagaucaacc acaccaagcu caucgacgag      960

uucgggacgg gccgcuucuu cagcgaggag gagcggaaga ccaucaucgg ccugcugcuc     1020

uccagcaagg agcugaagua cgggaccauc cgcaagaagc ugaacaucga ccccucccuc     1080

aaguucaaca gccugaacua cuccgccaag aaggagggcg agaccgagga ggagcgggug     1140

cuggacacgg agaaggccaa guucgcgagc auguucugga ccuacgagua cuccaagugc     1200

cucaaggacc gcaccgagga gaugcccguc ggggagaagg ccgaccuguu cgaccggauc     1260

ggcgagaucc ugaccgccua caagaacgac gacagccgcu ccagccggcu caaggagcug     1320

gggcuguccg gcgaggagau cgacggccuc cuggaccuga gcccggccaa guaccagcgc     1380

gugagccuca aggccaugcg gaagaugcag cccuaccugg aggacgggcu gaucuacgac     1440

aaggcgugcg aggccgccgg cuacgacuuc cgcgcccuca acgacgggaa caagaagcac     1500

cuccugaagg gcgaggagau caacgcgauc gugaacgaca ucacgaaccc cgucgugaag     1560

cgguccguga gccagaccau caaggucauc aacgccauca uccagaagua cggguccccg     1620

caggccguga acaucgagcu ggcccgcgag augagcaaga acuuccagga ccggaccaac     1680

cucgagaagg agaugaagaa gcgccagcag gagaacgagc gcgccaagca gcagaucauc     1740

gagcugggca agcagaaccc gaccggccag gacauccuga aguaccggcu cuggaacgac     1800

cagggcgggu acugccugua cuccgggaag aagauccccc uggaggagcu guucgacggc     1860

ggcuacgaca ucgaccacau ccugcccuac agcaucacgu ucgacgacuc cuaccgcaac     1920

aaggugcugg ucaccgcgca ggagaaccgg cagaagggga accgcacccc cuacgaguac     1980

uucggcgccg acgagaagcg gugggaggac uacgaggcca gcgugcgccu ccugguccgg     2040

gacuacaaga agcagcagaa gcugcucaag aagaacuuca ccgaggagga gcgcaaggag     2100

uucaaggagc ggaaccugaa cgacacgaag uacaucaccc gcguggugua caacaugauc     2160

cggcagaacc uggagcucga gccguucaac caccccgaga agaagaagca ggucugggcc     2220

gugaacgggg ccgugaccuc cuaccugcgc aagcgguggg gccugaugca gaaggaccgc     2280

agcaccgacc ggcaccacgc gauggacgcc gucgugaucg ccugcugcac ggacgggaug     2340

auccacaaga ucucccgcua caugcagggc cgggagcucg ccuacagccg caacuucaag     2400

uuccccgacg aggagaccgg cgagauccug aaccgggaca acuucacccg cgagcagugg     2460

gacgagaagu ucggggugaa ggucccccug ccguggaacu ccuuccggga cgagcucgac     2520

auccgccugc ugaacgagga ccccaagaac uuccuccuga cccacgccga cgugcagcgc     2580

gagcuggacu accccggcug gauguacggg gaggaggaga gccccaucga ggagggccgg     2640

uacaucaacu acauccgccc ccucuucgug ucccggaugc ccaaccacaa ggucaccggg     2700

agcgcgcacg acgccacgau ccgcuccgcc cgggacuacg agacccgcgg cguggugauc     2760

accaaggucc cccugaccga ccugaagcuc aacaaggaca acgagaucga gggcuacuac     2820

gacaaggaca gcgaccggcu gcuguaccag gcccucgugc gccagcugcu gcuccacggg     2880

aacgacggca agaaggcguu cgccgaggac uuccacaagc cgaaggccga cgggacggag     2940

ggccccgugg uccggaaggu gaagaucgag aagaagcaga ccuccggggu gaugguccgc     3000

ggcggcaccg ggaucgccgc caacggcgag auggugcgga ucgacguguu ccgcgagaac     3060

gggaaguacu acuucguccc cguguacacc gcggacgugg uccggaaggu gcugcccaac     3120

cgcgccgcca cgcacaccaa gccguacagc gaguggcggg ugauggacga cgccaacuuc     3180

gucuucagcc uguacucccg cgaccucauc cacgucaaga gcaagaagga caucaagacc     3240

aaccugguga acggcgggcu gcuccugcag aaggagaucu ucgccuacua caccggcgcg     3300

gacaucgcca cggccuccau cgccggcuuc gccaacgaca gcaacuucaa guuccggggg     3360

cugggcaucc agucccucga gaucuucgag aagugccagg uggacauccu ggggaacauc     3420

agcguggugc gccacgagaa ccggcaggag uuccacuga                            3459


<210>  1699
<211>  3462
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1699
augaagaaca uccugggccu cgaccugggg cugagcucca ucggcuggag cgugauccgc       60

gagaacuccg aggagcagga gcucgucgcc auggggagcc gggugguguc ccugaccgcc      120

gcggagcuga gcuccuucac ccagggcaac ggggucagca ucaacuccca gcgcacgcag      180

aagcggaccc agcgcaaggg cuacgaccgg uaccagcucc gccggacccu gcugcgcaac      240

aagcucgaca cccugggcau gcugcccgac gacagccucu ccuaccugcc gaagcugcag      300

cucugggggc ugcgggccaa ggccgugacg cagcgcaucg agcugaacga gcucggccgg      360

gugcugcugc accucaacca gaagcgcggg uacaagagca ucaaguccga cuucagcggc      420

gacaagaaga ucaccgacua cgucaagacc gugaagaccc gguacgacga gcugaaggag      480

augcgccuga cgaucgggga gcuguucuuc cggcgccuca ccgagaacgc cuucuuccgg      540

ugcaaggagc aggucuaccc ccgccaggcc uacguggagg aguucgacug caucaugaac      600

ugccagcgga aguucuaccc cgacauccug accgacgaga ccauccgcug cauccgggac      660

gagaucaucu acuaccagcg cccccucaag uccugcaagu accuggugag ccgcugcgag      720

uucgagaagc gguucuaccu gaacgcggcc ggcaagaaga cggaggccgg cccgaaggug      780

uccccgcgca ccagcccccu cuuccagguc ugccggcugu gggaguccau caacaacauc      840

guggugaagg accgccggaa cgagaucguc uucaucagcg ccgagcagcg cgcggcccug      900

uucgacuucc ucaacaccca cgagaagcug aaggggagcg accugcucaa gcugcugggc      960

cucuccaaga ccuacgggua ccggcugggc gagcaguuca agacgggcau ccaggggaac     1020

aagacccgcg uggagaucga gcgggcccug ggcaacuacc ccgacaagaa gcgccuccug     1080

caguucaacc ugcaggagga gagcuccagc auggugaaca ccgagaccgg ggagaucauc     1140

cccaugaucu cccucagcuu cgagcaggag ccccuguacc ggcuguggca cguccucuac     1200

uccaucgacg accgcgagca gcugcagagc gugcugcggc agaaguucgg caucgacgac     1260

gacgaggugc uggagcgccu cuccgccauc gaccugguca aggccggguu cggcaacaag     1320

agcuccaagg cgauccggcg cauccugccc uuccuccagc ugggcaugaa cuacgccgag     1380

gccugcgagg ccgccgggua caaccacagc aacaacuaca cgaaggcgga gaacgaggcc     1440

cgggcccugc ucgaccgccu gcccgccauc aagaagaacg agcugcggca gccgguggug     1500

gagaagaucc ucaaccagau ggucaacgug gucaacgccc ugauggagaa guacggccgc     1560

uucgacgaga uccgcgugga gcuggcgcgg gagcucaagc aguccaagga ggagcgcagc     1620

aacaccuaca aguccaucaa caagaaccag cgggagaacg agcagaucgc caagcgcauc     1680

guggaguacg ggguccccac ccggagccgc auccagaagu acaagaugug ggaggagucc     1740

aagcacugcu gcaucuacug cggccagccc guggacgugg gggacuuccu gcggggcuuc     1800

gacgucgagg uggagcacau cauccccaag agccuguacu ucgacgacuc cuucgccaac     1860

aaggugugca gcugccgcag cugcaacaag gagaagaaca accggaccgc cuacgacuac     1920

augaagucca agggcgagaa ggcccucagc gacuacgucg agcgcgugaa cacgauguac     1980

accaacaacc agaucuccaa gaccaagugg cagaaccugc ugaccccggu ggacaagauc     2040

agcaucgacu ucaucgaccg gcagcuccgc gagucccagu acaucgcgcg gaaggccaag     2100

gagauccuga cgagcaucug cuacaacguc accgccaccu ccgggagcgu gaccuccuuc     2160

cugcgccacg uguggggcug ggacacgguc cuccacgacc ugaacuucga ccgguacaag     2220

aagguggggc ugaccgaggu gaucgagguc aaccaccgcg gcagcgugau ccggcgcgag     2280

cagaucaagg acugguccaa gcgguucgac caccgccacc acgccaucga cgcgcucacc     2340

aucgccugca ccaagcaggc cuacauccag cggcugaaca accugcgcgc cgaggagggg     2400

cccgacuuca acaagaugag ccucgagcgc uacauccagu cccagcccca cuucagcgug     2460

gcccaggucc gggaggcggu ggaccgcauc cugguguccu uccgggccgg caagcgcgcc     2520

gucacgcccg gcaagcggua cauccgcaag aaccggaagc gcaucagcgu gcaguccgug     2580

cugaucccgc gcggggcccu cagcgaggag agcgucuacg gcgugaucca cgugugggag     2640

aaggacgagc aggggcacgu cauccagaag cagcgggccg ugaugaagua ccccaucacc     2700

uccaucaacc gggagaugcu ggacaaggag aagguggucg acaagcgcau ccaccggauc     2760

cugagcggcc gccucgcgca guacaacgac aaccccaagg aggccuucgc caagcccgug     2820

uacaucgaca aggagugccg gaucccgauc cgcaccgugc ggugcuucgc caagcccgcc     2880

aucaacaccc uggucccccu gaagaaggac gacaagggca accccguggc gugggugaac     2940

ccggggaaca accaccacgu cgccaucuac cgcgacgagg acggcaagua caaggagcgg     3000

accgugacgu ucugggaggc cguggaccgc ugccgcgucg ggauccccgc caucgugacc     3060

cagcccgaca ccaucuggga caacauccuc cagcggaacg acaucuccga gaacgugcug     3120

gagagccugc cggacgucaa guggcaguuc gugcucuccc ugcagcagaa cgagauguuc     3180

auccugggca ugaacgagga ggacuaccgc uacgcgaugg accagcagga cuacgcccuc     3240

cugaacaagu accuguaccg ggugcagaag cucagcaagu ccgacuacag cuuccgcuac     3300

cacaccgaga cguccgucga ggacaaguac gacgggaagc ccaaccugaa gcugagcaug     3360

cagaugggca agcucaagcg gguguccauc aagagccugc ugggccucaa cccccacaag     3420

gugcacaucu ccguccuggg ggagaucaag gagaucagcu ga                        3462


<210>  1700
<211>  3474
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1700
augaacuacu acaacggcuu caucaucacc gagaacgcca ugaccagcaa gcaggagaag       60

cacggggccc ugacguucgg ccucgacauc gggaucgcgu ccgugggcug ggccguccug      120

ggggagagcc gcaucgugga ccugggcgug cggugcuucg acaaggccga gaccgccaag      180

gagggcgagu cccucaaccu gacccgccgg auggcccgcc ugcuccggcg ccggcugcgc      240

cggcgcgcgu ggcggcugac caagcucgcc cgccugcuga agcgggaggg gcucaucgcc      300

gacgccaacc uguucaagca caagaucgag aagaacacgc ccguccccaa gagcuccugg      360

cagcugcgcg uggacggccu cgaccggcag cugaacgcgg aggagugggc ccgcgugauc      420

uaccaccugu gcaagcaccg gggguuccac uggaucagcc gcgccgagga gaaggccgcc      480

gagggcgacu ccaaggggga gggcggcaag gucaagcagg ggcucgcggg caccaagaag      540

cugaugcagg agaaguccua ccggaccgcc gccgagaugg ugcuggccga guuccccgac      600

gcccagcgca acaagcaggg ggacuacagc aaggcgcuca gccggauccu gcugggcgac      660

gagcucaagg cccuguucga gcaccagcgc aagcugggca accccguggc caccgagaag      720

cucgaggccg ccauccuggg gaacggcgac aagaagugcg ggcuguucug ggcgcagaac      780

cccgcccucg ccggcaacaa gcugcuggag augcucgggc gcugcacguu cgagaagacc      840

ggcggcgacg acgggaaggg cgaguaccgg gccccgaagg ccuccuucag cgcggagcgc      900

cacgucuggc ugacccaccu gaacaaccuc cggaucgugg uggacgggac cacgcgcccc      960

cugaacgagg ccgagcggcg cgucgcccug cccaugcccu accagcaggc cggcgaccuc     1020

uccuacaagc agcucggggg cgcgcugauc aaggagggcu uccuccagaa ggacggguuc     1080

aaguucgccg gccugccgua ccccaccaag caggccagcg aggagaaggc caaggacccc     1140

gagaccuccg ugcugugcaa gcuccccgcc uggcacaccc ugcggaagac gcuggagaag     1200

cacgggcucg agagcgagug gcagaagcug uccaccgacg cgcuggacgg caagccgcag     1260

cugcucgaca acaucgccug ggugcugagc gucuacaagg acgacgacga ggugaagacc     1320

gagcugggga agcucaaccu gcccaacacc gagaagauga ucgacgcccu gcucgacauc     1380

cgcuucgaca aguucuccaa ccugagccug aaggcccuca gcaagaucgu gcccuucaug     1440

gagcagggcc agcgguacga cgaggccguc gcgaacaucc ccgaguacgg ccaccacucc     1500

cagcugcaca aggugggggc cggcgagcac aaguaccugc cgcccuucua caaggagcgc     1560

gacaagacgg ggcggauggu guucaacgac gacauggaca ucccccgcaa ccccgucgug     1620

cuccgggccc ugaaccaggc ccgcaaggug gucaacgccc ugaucaagga guacggcagc     1680

ccgcacgagg ugcacaucga gauggcgcgg gaccucuccc gcccccugga cgagcggcgc     1740

gacaucgaga agcugcagaa ggaguucggc gagcggaaca agaaggacaa ggagcuguuc     1800

gcccaggacu ucaacaucgu cgggaccgug aagggcaagc aguucgagaa guaccagcug     1860

uaccgcgagc agcaggggaa gugcaucuac cccagcaugg acgagaagca gggcaucugc     1920

ugcuacuccc ugaagccccu cgaccugcac cgcgugcugu acgaggaggg guacgccgag     1980

aucgaccacg cccucccgua cagccggucc uucgacgaca gcaagaacaa ccgcguccug     2040

gugcuggcga aggagaaccg ggacaagggc aaccgcaccc ccuucgagua ccucggcgac     2100

aaggacaacu ccgagcccug gcggcaguuc guggccuucg ucgaggggaa caaggccuac     2160

cgccuggcca agcggagccg ccugcuccgg aaggacuucg gcgacaagga ggccaaggag     2220

uuccgcgagc ggaaccugaa cgacacccgc uacaucugca aguucuucaa gaacuacgug     2280

gagcaguacc ugcagcucca cgaggacucc gaggcgaagc ggugcguggu ccugagcggg     2340

cagaugacgu ccuuccugcg cgcccggugg ggccucguga aggugcgcgg ggacagcgac     2400

cggcaccacg cccuggacgc cgccgucgug gcggccugcu cccacggcau ggugaagcgc     2460

cugagcgacu acucccggcg caaggagcuc gagcaggucc gggagggcuu cguggacgug     2520

gacaccgggg agaucgucaa ccccgccaug uuccagcagg uccaggagca cuucccgaac     2580

cccuggcccc acuuccgcga cgaggugaag cugcgccuga gcaucgacga cccgacccuc     2640

cugcgguccg agauggagaa guucggcacc uacacgcccg agagccugga ggcccuccgc     2700

ccccuguucg ugagccgggc cccgcagcgc cggaacuccg gggcggccca caaggacacc     2760

aucuacgccc agccgaagcg ccugguggcc cagggcgggg ugacccagaa ggucgcgcuc     2820

agcucccuga cccugaagga ccucgacaag cugaucgacc cccaccggaa cgagcgccug     2880

uacgccgcca uccgggcccg ccucgagcag cacggcggca agggggacaa ggccuucccc     2940

gcgagcaacc cccugcggaa gccggacucc gagggcaacc ccacggggcc caucgugcgc     3000

ggcgugacca uggucaucga caagcugagc gggaucccca uccggggcgg caucgccaag     3060

aacgacucca uggugcgcgu ggacgucuuc accaaggcca acaaguucca ccucgugccg     3120

guguacgucc accacagcgu gcuggggcug cccaaccagg ccaucguggg cgggaaggag     3180

gaguccgagu ggacccagau cgacgacagc uucgacuggc acuucuccau cuaccccaac     3240

gaccucaucc gggucacgcu gaagcaggag ggccgcuucg gcuacuacgc cgggugcgac     3300

cggagcaccg cggccaucca ccugugggcc cacgaccgca accagcaggu cggcaaggac     3360

gggcucaucc gguccaucgg cgugaagacc gcccugagca ucgagaaguu caacguggac     3420

gugcugggga accgcuaccc cgccccgccc gagaagcgcc gggaccucgc guga           3474


<210>  1701
<211>  3486
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1701
augaagaaga uccugggccu cgaccugggg agcgccucca ucggcugggc ccugguggag       60

gagaccgagc agaugcagua caacaucguc agccucgggu cccgcaucau ccccuacaac      120

ggcaccgagg ggcaggaguu cgagaagggc acgggcgaga gccggaacuc ccugcgcacc      180

aaggcgcgga ccgcccgcaa gggguacgac cgguaccagc ugcgccggaa gaaccucgag      240

aagauccugc ugcagaacgg cauguacccg aacgagcagg uccgcgcccu ccagaagcug      300

gagcugugga agcuccgggc cgacgccccc aacgugcaga ucagccugcc cgagcugggg      360

cgccuccugu ucuggcugaa ccagaagcgg ggcuacaagu ccagccgcuc cgacgcgaac      420

cucgacaaga aggacaccga guacguggcc acggucaaga gccggcacca gaagaucaag      480

gacgccgggc agaccguggg ccaguacuuc uacggccaga uccuggagaa cgaguaccac      540

cgcaucaagg agcaggucuu cccccgggag gccuacaucg aggaguucga cgcgaucgug      600

gcccgccaga aggaguacua cccgaccauc cugaagcagg agcucaucga caagauccgg      660

aacgagguga ucuacuucca gcgcgggcug aagucccaga agggccuggu gagcgugugc      720

gaguucgagg ggcgggaguu caacggccac uucggcgggc ccaagguggc ccccaagucc      780

agccccaucu uccagcucgg caagaucugg gagaacauca acaacaucaa gaucgccgac      840

aucgagcuga ccgccgagca gaagcugguc uucuucaacu accucaacug ccacgagcgc      900

aucacggagg cgaagauccg ggagcugcug aagcucucca aggacagccg ccugaacggg      960

cagcugaaga acggccucac cgggaacaag accuacuccc agaucgccag cugcuucggc     1020

aacaagaucg gcgaguacga gcgccuguuc aaguucgagc uggaggugau cgaguccgag     1080

aagcucgagu acaccuacca ccccaagagc cacaucccgg ugggggagaa gcggcgcaag     1140

uacaucagcg agaacgucga gaaggagccc uacuaccggc uguggcacac gaucuacucc     1200

aucaaggaca aggacgagug ccgcgccgcc cugaucaaga acuucggcau cgaggaggag     1260

aucgccgaga agcucgcgag ccuggacuuc uccgccgggg gcuucgggaa caagugcgcc     1320

aaggugaucc ggaagauccu gcccuaccuc auggagggcg acaaguacaa cguggccaug     1380

gacuacgccg gcuacaacca cagcuucucc cagaccaagg acgagaaccu gcagcgcguc     1440

cugcucgaca gcaucaacca ccucccgaag aacucccugc ggcagcccau cguggagaag     1500

auccucaacc agaugaucaa ccuggugaac gaggucauca agcagugggg gcgccccgac     1560

gagauccggg uggagcuggc gcgcaagcug aagcagagca agaccgagcg gaacgacgac     1620

gacaacuuca ugcgcaagcg ggagcgcgag aacgagauca ucaaggccga ccucgccaag     1680

uacggccuga aggccacccg gaacaacgug aucaaguggc gccuguacca cgagaucgac     1740

gacaaggaca agaagcucaa cgcccggugc aucuacugcg ggcagcccau cucccugacg     1800

gaggcgaucc ugggcaacaa cgucgacgug gagcacauca ucccgaagga caagcuguuc     1860

gacgacagcc aguccaacaa gacccuggcc caccgcagcu gcaacuccgg gaagggcaac     1920

aagaccgccu acgacuucau gaaggacaag ccccaguucg aggaguacgu cgcccgggug     1980

gaggagcugu acaagaagca ccucaucagc cugaccaaga agcgcaaguu ccugaugucc     2040

gaggacaaga uccccagcga cuucaucgac cgccagcucc gggaguccca guacaucgcg     2100

cgcaagagcc gggagauccu gcagacggug ugccgcaacg ucugguccac cgacggcggg     2160

gugaccgccc accuccggaa gcuguggggc ugggacgacg ugaccaugaa ccugcagaug     2220

cccaaguaca aggggaucgg caugacggag aucgucgagu gggagagcaa ccacggcaag     2280

aaccagcaca cccaggaggu gaucaaggac uggagcaagc gcgacgacca ccggcaccac     2340

gccaucgacg cccugguggu cgccugcacc cgccaggggu acauccagcg gaucaacacc     2400

cucaacucca gcgagacgaa ggacgagaug caccgcaagg uggcgggcuc ccugguggag     2460

uucaaccaga agcacacccu gcucaacaag uaccugauca ccgagaagcc guucaccacg     2520

agcgaggucg aggacguggu guccaagauc cuggucagcu ucaaggccgg gaagaaggcc     2580

gccacccccg gcaagcggaa gguggggaag cgcggcaaca agcaggucgu gcaggacggc     2640

aucaucaucc cccgcggggc ccuccacgcg gacuccguga gcggcuccau caagcaguac     2700

gaccccaccg ggaacuucga gaaccacaac gugaucaagu acaaggucgg cguggggaag     2760

ggcuaccugu ucgacggcaa ggacgacaag aagaagaucg acaagaccau cgacugcauc     2820

guggacaaga agauccggga ccugaucaag cagcgggccg aggagagcaa cuacucccuc     2880

aagaagaugu ucgagacgcc gauccuguuc aaccagcgcg ccgucuucag cgugcggugc     2940

aagaccggga acaaggccgu gguccccauc cgcaacggcu acguggugcc cgacaacaac     3000

caccacaccg ccaucuacaa guacgacggg ggccugaagg agcagugcgu cucccucaug     3060

caggcgaucg agcggaagcg cuacaaccug cccaucauca ucaagaaccc gaaggagauc     3120

uacgacaaga ucaccgagau cgccaacauc aaccagcucc uguucgacag ccugcccaag     3180

cuggacuggg aguucgugac cacgcuccag aagaacgaca uguucauccu ggggauggac     3240

uacuccgcca ccgaggaggc ccuggcggac ggcaacuacc ggcuccugag cgaguaccug     3300

uacaaggugc agaacaucuc cgacggcauc uaccgccucu gccaccacac caucaccaag     3360

uucgaccuga gcaaggccaa caagcccgac aagcgcuucu acaacaucca gagccugaag     3420

gccuggaaca cgcucaaccc gcagaagguc aagaucaaca uccuggggca gaucgagcgg     3480

caguga                                                                3486


<210>  1702
<211>  3501
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1702
augcacgugg agaucgacuu cccccacuuc agccgcggcg acucccaccu ggccaugaac       60

aagaacgaga uccuccgggg gagcuccguc cuguaccgcc ugggccucga ccuggggagc      120

aacucccugg gcugguucgu gacccaccuc gagaagcgcg gggaccggca cgagccggug      180

gcccugggcc ccggcggggu ccggaucuuc cccgacggcc gcgaccccca gagcgggacc      240

uccaacgcgg uggaccggcg cauggcccgg ggcgcccgca agcggcgcga ccgguucgug      300

gagcgccgga aggagcugau cgccgcccuc aucaaguaca accugcugcc ggacgacgcg      360

cgcgagcggc gcgcccucga gguccuggac cccuacgccc ugcggaagac ggcccucacc      420

gacacccugc ccgcgcacca cguggggcgc gcccuguucc accucaacca gcggcgcggc      480

uuccagagca accgcaagac cgacuccaag cagagcgagg acggcgccau caagcaggcc      540

gccucccggc uggcgacgga caaggggaac gagacccugg gcguguucuu cgccgacaug      600

caccuccgca agagcuacga ggaccggcag accgccaucc gcgccgagcu gguccggcug      660

gggaaggacc accucaccgg caacgcccgc aagaagaucu gggcgaaggu gcggaagcgc      720

cuguucggcg acgaggugcu gccccgggcc gacgccccgc acgggguccg cgcccgggcc      780

acgaucaccg gcaccaaggc guccuacgac uacuacccca cccgcgacau gcuccgggac      840

gaguucaacg ccaucugggc cgggcagagc gcccaccacg ccacgaucac cgacgaggcg      900

cgcaccgaga ucgagcacau caucuucuac cagcggcccc ugaagcccgc caucgugggc      960

aagugcaccc uggacccggc cacgcgcccc uucaaggagg accccgaggg guaccgggcc     1020

cccugguccc acccgcucgc gcagcgcuuc cggauccuga gcgaggcccg caaccuggag     1080

auccgcgaca ccggcaaggg cucccggcgc cucaccaagg agcagagcga ccuggugguc     1140

gccgcccugc ucgccaaccg ggaggugaag uucgacaagc ugcgcacccu gcucaagcug     1200

cccgcggagg cccgguucaa ccuggagucc gaccgccggg ccgcccucga cggggaccag     1260

acggccgcgc gccugagcga caagaagggc uucaacaagg ccuggcgggg guucccgccc     1320

gagcgccaga ucgccaucgu ggcccggcug gaggagaccg aggacgagaa cgagcucauc     1380

gccuggcugg agaaggagug cgcgcuggac ggcgccgccg ccgcgcgcgu cgccaacacc     1440

acccuccccg acgggcacug ccggcugggc cugcgcgcca ucaagaagau cgugcccauc     1500

augcaggacg gccuggacga ggacggggug gccggcgccg gguaccacau cgcggccaag     1560

cgggccggcu acgaccacgc caagcucccc acgggggagc agcugggccg ccugcccuac     1620

uacggccagu ggcuccagga cgccgucgug gggagcggcg acgcgcggga ccagaaggag     1680

aagcaguacg ggcaguuccc gaaccccacc gugcacaucg gccugggcca gcugcgccgg     1740

gucgugaacg accucaucga caaguacggg ccgcccaccg agaucuccau cgaguucacc     1800

cgcgcccuga agcugagcga gcagcagaag gccgagcggc agcgcgagca gcgccggaac     1860

caggacaaga acaaggcccg cgccgaggag cucgcgaagu ucggccggcc cgccaacccc     1920

cgcaaccugc ugaagaugcg gcucugggag gagcuggccc acgacccccu ggaccgcaag     1980

ugcgucuaca cgggggagca gaucuccauc gagcggcucc ugagcgacga gguggacauc     2040

gaccacaucc ugccgguggc caugacccuc gacgacuccc ccgcgaacaa gaucaucugc     2100

augcgcuacg ccaaccggca caagcgcaag cagaccccca gcgaggccuu cggcuccagc     2160

cccacccugc aggggcaccg guacaacugg gacgacaucg ccgcccgcgc gacgggccug     2220

ccgcggaaca agcgcuggcg guucgacgcc aacgcccgcg aggaguucga caagcggggc     2280

ggguuccucg cccgccagcu gaacgagacc ggcuggcugg cccggcucgc gaagcaguac     2340

cugggggccg ucaccgaccc caaccagauc uggguggugc ccggccgccu gaccuccaug     2400

cuccgcggga aguggggccu gaacggccug cuccccagcg acaacuacgc cgggguccag     2460

gacaaggccg aggaguuccu ggccuccacg gacgacaugg aguucagcgg cgugaagaac     2520

cgggcggacc accgccacca cgccaucgac gggcugguga ccgcccucac cgaccggucc     2580

cugcugugga agauggccaa cgccuacgac gaggagcacg agaaguucgu caucgagccg     2640

cccuggccca ccaugcgcga cgaccucaag gcggcccugg agaagauggu ggugagccac     2700

aagcccgacc acggcaucga ggggaagcug cacgaggacu ccgccuacgg cuucgucaag     2760

ccgcucgacg ccaccggccu gaaggaggag gaggcgggga accuggugua ccggaaggcc     2820

aucgagagcc ucaacgagaa cgagguggac cgcauccggg acauccagcu gcgcacgauc     2880

guccgggacc acgugaacgu ggagaagacc aagggcgucg cccuggccga cgcccuccgc     2940

cagcugcagg cgcccuccga cgacuacccc caguucaagc acgggcugcg gcacgugcgc     3000

auccucaaga aggagaaggg cgacuaccug gugcccaucg ccaaccgggc cagcgggguc     3060

gccuacaagg ccuacuccgc gggcgagaac uucugcgugg agguguucga gaccgccggc     3120

gggaaguggg acggcgaggc cguccgccgg uucgacgcca acaagaagaa cgccgggccg     3180

aagaucgcgc acgcgcccca guggcgcgac gccaacgagg gcgccaagcu ggugaugcgg     3240

auccacaagg gcgaccucau ccgccuggac cacgaggggc gggcccgcau cauggugguc     3300

caccggcugg acgccgccgc cggccgcuuc aagcucgccg accacaacga gaccgggaac     3360

cuggacaagc gccacgcgac gaacaacgac aucgaccccu uccgguggcu gauggccagc     3420

uacaacaccc ucaagaagcu ggccgccgug cccgugcgcg ucgacgagcu gggccgggug     3480

uggcgcguga ugccgaacug a                                               3501


<210>  1703
<211>  3507
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1703
auggcccgcc ccgccuuccg ggcgccgcgc cgggagcacg ugaacggcug gacccccgac       60

ccccaccgca ucagcaagcc cuucuucauc cuggucuccu ggcaccuccu gagccgggug      120

gugaucgacu ccagcuccgg gugcuucccg ggcaccagcc gcgaccacac ggacaaguuc      180

gccgaguggg agugcgccgu ccagcccuac cggcuguccu ucgaccucgg gaccaacagc      240

aucggcuggg ggcugcugaa ccucgaccgc cagggcaagc cccgggagau ccgcgcccug      300

ggcucccgga ucuucagcga cgggcgcgac ccccaggaca aggccucccu ggcgguggcc      360

cggcgccucg cccggcagau gcgccggcgc cgggaccgcu accugacccg gcgcacccgc      420

cugaugggcg cccucgugcg guucgggcug augccggcgg accccgccgc ccgcaagcgg      480

cuggaggucg ccguggaccc cuaccucgcc cgcgagcggg cgacgcgcga gcggcuggag      540

cccuucgaga ucggccgcgc ccuguuccac cucaaccagc ggcgcgggua caagccggug      600

cggaccgcca ccaagcccga cgaggaggcc ggcaagguca aggaggccgu ggagcgccug      660

gaggcggcca ucgccgccgc cggcgcgccc acccuggggg ccugguucgc cuggcggaag      720

acgcgcggcg agacccuccg ggcccgccug gccgggaagg gcaaggaggc ggccuacccc      780

uucuacccgg cccggcgcau gcuggaggcc gaguucgaca cccucugggc ggagcaggcc      840

cggcaccacc ccgaccugcu gaccgccgag gcccgcgaga uccuccgcca ccggaucuuc      900

caccagcgcc cccugaagcc cccgcccgug ggccggugca cgcuguaccc cgacgacggg      960

cgcgcgcccc gggcccuccc gagcgcccag cgccugcggc uguuccagga gcucgccucc     1020

cugcgcguca uccaccugga ccucagcgag cggccccuga cccccgccga gcgcgaccgg     1080

aucguggccu ucgugcaggg ccggcccccg aaggcggggc ggaagcccgg caagguccag     1140

aaguccgugc ccuucgagaa gcugcgcggg cuccuggagc ugccgcccgg caccggcuuc     1200

agccucgagu ccgacaagcg gcccgagcug cugggggacg agaccggcgc ccgcaucgcc     1260

cccgccuucg ggccgggcug gacggcccuc ccccuggagg agcaggacgc gcugguggag     1320

cuccugcuga ccgaggccga gcccgagcgg gccaucgccg cgcugaccgc ccgcugggcc     1380

cucgacgagg ccaccgccgc gaagcuggcc ggggccacgc ugcccgacuu ccacggccgg     1440

uacggccgcc gggccgucgc cgagcuccug ccggugcugg agcgcgagac ccgcggggac     1500

cccgacggcc gggugcgccc cauccggcuc gacgaggcgg ucaagcugcu gcgcgggggc     1560

aaggaccaca gcgacuucuc ccgggagggg gcccuccugg acgcccugcc cuacuacggc     1620

gccgugcucg agcgccacgu ggccuucggc accgggaacc cggcggaccc cgaggagaag     1680

cgggucggcc gcguggccaa ccccaccguc cacaucgccc ugaaccagcu gcggcaccuc     1740

gugaacgcca uccuggcgcg ccacgggcgg cccgaggaga ucgugaucga gcuggcccgc     1800

gaccucaagc ggagcgccga ggaccgccgg cgcgaggaca agcggcaggc cgacaaccag     1860

aagcgcaacg aggagcggaa gcgccugauc cugagccucg gcgagcgccc gacgccccgg     1920

aaccugcuga agcuccgccu gugggaggag cagggccccg ucgagaaccg gcgcugcccc     1980

uacuccgggg agaccaucag caugcggaug cugcucuccg agcaggucga caucgaccac     2040

auccugccgu ucagcguguc ccuggacgac agcgccgcga acaagguggu gugccuccgc     2100

gaggccaacc ggaucaagcg caaccggucc cccugggagg ccuucggcca cgacagcgag     2160

cgcugggccg ggauccuggc ccgggcggag gcccugccca agaacaagcg cuggcgguuc     2220

gcccccgacg cccucgagaa gcuggagggc gagggcggcc ugcgcgcccg gcaccucaac     2280

gacacccgcc accugucccg gcuggcggug gaguaccucc gcugcgucug cccgaaggug     2340

cgggugagcc ccggccgccu gaccgcccug cuccggcgcc gcugggggau cgacgccauc     2400

cuggccgagg ccgacgggcc cccgcccgag guccccgcgg agacgcugga ccccuccccg     2460

gccgagaaga accgggccga ccaccgccac cacgcccucg acgcgguggu gaucgggugc     2520

aucgaccgga gcauggucca gcgcgugcag cuggccgccg ccuccgccga gcgggaggcg     2580

gccgcccgcg aggacaacau ccggcgcgug cuggagggcu ucaaggagga gcccugggac     2640

ggguuccggg ccgagcucga gcgccgggcc cgcaccaucg ucgugagcca ccggcccgag     2700

cacggcaucg gcggggcgcu gcacaaggag accgccuacg gccccgugga cccgcccgag     2760

gagggguuca accuggucgu gcgcaagccc aucgacggcc ucuccaagga cgagaucaac     2820

agcgugcggg acccccgccu gcggcgcgcc cugaucgacc ggcucgccau ccgccgccgg     2880

gacgccaacg acccggcgac cgcccuggcc aaggccgcgg aggaccuggc cgcccagccc     2940

gccucccgcg ggauccggcg cguccgggug cucaagaagg agagcaaccc cauccgcgug     3000

gagcacggcg gcaaccccuc cgggccgcgg agcggcgggc ccuuccacaa gcugcugcuc     3060

gccggcgagg uccaccacgu ggacguggcg cugcgcgccg acgggcggcg cugggucggc     3120

cacuggguga cgcuguucga ggcccacggc gggcggggcg ccgacggggc cgcggccccg     3180

ccccgccucg gcgacggcga gcgguuccug augcgccugc acaaggggga cugccucaag     3240

cuggagcaca agggccgggu gcgcgucaug caggucguga agcuggagcc cagcuccaac     3300

agcguggugg uggucgagcc ccaccaggug aagaccgacc gguccaagca cgugaagauc     3360

agcugcgacc agcuccgcgc ccgcggggcc cgccggguca ccguggaccc ccugggccgc     3420

gugcgcgucc acgcccccgg ggcgcgggug ggcaucggcg gggacgccgg ccgcaccgcc     3480

auggagccgg ccgaggacau cuccuga                                         3507


<210>  1704
<211>  3513
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1704
augagcaagu acaacguggg ccuggacauc gggaucgccu ccgucggcug gagcaucgug       60

gacgccgaca agaagaagau caucgaccuc gggucccgca ucuucagcuc cggcaacgcg      120

gccgccaacc aggagcggcg cggguuccgg ggcacccgcc ggcugauccg ccggcgcaag      180

aaccggcuga ccgacgugau caacuucuuc ggcgagaaga aguucaucga cacgaagguc      240

guggacaaga acgggcacgu gcacuacaag uucagcguca acaacaccga gucccccuac      300

gagcuccgcg ugaagggccu gagcgagauc cugaccaagc aggagcucgu gaucgcccug      360

uaccacaucg ucaagcaccg ggggaucucc uacgaccugg gcgaccucga ggacgacggg      420

accagcggcg ugacggacua caagaccucc aucaacauca accgccagcu gcugaacgag      480

aagaccgugg gccagaucca gcucgagcgg cugaacgagu ucgggaaggu ccgcggccag      540

guggaggaga acgagaacgu gacccugcuc aacgucuucc cgagcgccgc guacgugaag      600

gaggccacgc agauccugac caagcagcgg gaguucuacc ccgagaucac cgacgacuuc      660

aucgccaagu ucaugcuguu caucacccgc aagcgggacu acuucguggg gcccggcaac      720

gagaaguccc gcacggacua cggcaucuac aagaaggacg ggcggacccu cgacaaccug      780

uucgacgagc ugaucggcau cgacaagauc aacggggaga agcgcgccag cgcguccagc      840

cucaccgccc agaucuacaa caugcugaac gaccugaaca accucaccgu ccccaacacg      900

gaggacggcc ggcugaccau ccagaccaag aaggagaucc uggaggccgc caagaccacg      960

aacgggcugu ucggcaucgc ccagaucugc aagaucaucg gcugcaagaa ggacgacauc     1020

aagggguucc gcaucgacaa guccgagaag aagaucaugc acacccugag cggcuaccgc     1080

aaguuccgga aggagcuggc ggacgugaac aucgaggcca acgaccucaa caucaaccug     1140

gugaacaaga ucgccgacau ccugacccuc aacaccgaga aggccgagau ccgcaagcag     1200

cuggacaagu ccaacaugga caucagcaac gagaugaagg agaucgucau caacaagaac     1260

aaggaguucc ugguggacgg gcgggccacg uggcacagcu ucuccuacaa gacccucaac     1320

cugcugaucc cggagcuccu gaacaccccc gaggagcaga ugaccauccu gacgcgccuc     1380

ggccugguga agcccaacaa caagaaguac gaggggcuga agaacauccc cgcgcggcgc     1440

aucaccgagg agaucuacaa cccggucgug gcccgggcca cccgcgaggc cgucaacgug     1500

uucaacgcca ucaccaagaa ggugggccgg gacaacauca aggacgucau caucgagcuc     1560

ccccgcgagg acaacgaggu ggacaucaag aagggcaucg ugaagcggca gaagcagaac     1620

gaggucgaga aggagacggc ggacgcccug gugaagcagc acaugaccau cagcgacucc     1680

gcccugaucg cccaguaccg caagauccgg gggcucagcc agaaggugcg cuacugguac     1740

cagcaggaca ccaucugccc cuacugcggc aagaagaucg aggccgucca gcugaucaac     1800

aacaacgacu ccuucgaggu ggaccacauc gugcccauca gcgucuccuu cgacgacagc     1860

cagaacaaca aggugcuggu gcacucccag ugcaaccagg cgaaggagaa gcagaccccg     1920

cugggguggc ucaacaacgg cgggggcuuc ggccagagca aggccgacua caucgcccgg     1980

gucaagucca acaagaacua cgccaagaac aagaucgaca accugcugaa cgagacggac     2040

cucaacgaca ucgcgaccca gcgcggguuc auccagcgga accugaacga cacccgcuac     2100

gccagccgga ucgugcugaa cgaguucuac uccuucuucc gcagcaacaa ccucuccacc     2160

aaggugaagg ucgugcgcgg caaguggacc agccagaugc ggaagaagug gaacggggug     2220

ggcggccugu ccaagacgcg cgacaccuac caccaccacg ccaucgacgc cugcaucauc     2280

gccugcuucc cccugcucaa ggcguucgac aaggccauca agcugaucga cgucgacggg     2340

gagaccggcg agauccugca ggacaagaag gccaucaagc ucgcccagga ggccgagauc     2400

guggagaaga ucagcgugau caagaacaag aaguucgaga acauggucaa cgagaucuac     2460

aacuuccccc uguucaagca ggucgagcug gcgaacgaca ucaccaaccu cgagaacccc     2520

gugaaguucu cccaccacgu ggacaagaag gccaaccggg ccguggccaa ccagacgauc     2580

uacgggaccc gcaucaagga gaaggaggug accaagcggg gcaagaagga gaagauccag     2640

gagcgcuaca cccuggggac gaucaagaac aucuacgaca ucgacggcuu caugaacuuc     2700

aagaagcugu acgacaagau cagcaagaac gacgacguca aguuccucau gcagaagaac     2760

gacccgaaga ccugggagaa gcugaucgac aucuucaaca gcuaccccga cuccgaggag     2820

gugacccagg uggacggcaa ggucaagcgg guggccguga gccccuucaa gcuguacuac     2880

cgcgagaacg gguucaucac caaguacucc aagcacaacg acggccccaa ggucgugagc     2940

aucaaguacu acgacuccaa gguggggagc cacaucgaca ucacgccgaa ggacgcgcgg     3000

aacaagguca uccuccaguc ccugaagccc uggcgcaccg acguguacuu caacagcauc     3060

acccagcagu acgagcugcu cggccugaag uacuccgacc ugaaguucac caacggggug     3120

uacggcauca cgcgggacgc cuacgagaag cucaagcacg gcgucagcga ggacggggug     3180

accgccugga agcccaucuc caagaacagc gaguuccgcu ucucccugua ccggaacgac     3240

cgcgugcggg ucaucgacga gaagggcgac agcaucgagc uccucuucgc cucccgcacc     3300

accagcaacg agggguacgu ggagcugaag cccaucuaca aggcgaaguu cgacuccaag     3360

gaggaggugg gcuucuacgg gcuggucagc aacggccggc ucaucaagaa gaugagcaag     3420

ggcggguaca agcuguacaa ggugaacacg gacauccugg gcaagccgua cuacgugacc     3480

cgcgaguccg acgagcccaa gcucagccug uga                                  3513


<210>  1705
<211>  3516
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1705
auggccaagu acagcguggg ccuggacauc gggaucucca gcgucggcug ggccaucauc       60

gacaucgaga ccaagcagau cgugaaccuc gggucccgca ucuuccccag cggcaacgcg      120

gccgggaacc aggagcggcg cggcuuccgg ggcauccgcc ggcugauccg ccggcgcaag      180

aaccggcuga ccgaccucaa gaagcugcug ggggagacgu ucuucuccga ccucaagaag      240

aucgacagca acggcuacga guacuacgac aucgacuucc agaucaacgc caacccguac      300

gagcugcgcg ugaaggggau caccgagaag cuguccaaga aggagcucgc cgucgugcug      360

caccacauca ucaagcaccg gggcaucagc uacgaccugg gggaccucga ggacgacggc      420

accuccggcg ugagcgacua caagaacucc aucaacauca accgccagcu gcugcgggag      480

aagaccgucg ggcagaucca gcucgagcgc cugaucgagu ucaaccaggu gcacggccag      540

gugaagaagg ccgaggacgu cagccugcuc aacguguucc ccuccagcgc guaccgggac      600

gaggccaaca agaucaugau caagcagcag gaguucuacu ccgagaucac gaacagcuuc      660

auggagcagg ucgaccagcu ggugucccgc aagcgggacu acuucaucgg gcccggcaac      720

gagaaguccc gcaccgacua cggcaucuac aagagcgacg ggcggacccu ggacaaccuc      780

uucgccgagc ugaucggcac cgacaagauc aacggggaga agcgcgccag cgcgagcucc      840

cugacggccc agguguacaa ccuccugaac gaccugaaca accuccacgu gagcaacacc      900

gaggacggca agcugaccac cuccacgaag aaggagauca ucaaggccgc caagaccacc      960

gacggggucu ucggcaucag ccagaucugc aagaucaucg gcugcaagaa ggaggagauc     1020

accggguucc ggcaggacaa guccgagaag aaggugaucc acacgcuggc cggcuaccgc     1080

aagauccgca aggcgcucgc caccaucaac aucgaggcca acgagcugaa ccccaccgug     1140

uucgaccaga ucgccgacau ccugacccuc aacacggaga aggccgagau ccggaagcag     1200

cuggacaagg uccagcugga caucaccgac gagaugaagg agcucgugau cgacaagaac     1260

aaggacuucc ugguggacgg gcgcgcgacc uggcacagcu ucuccuacca gacccugaac     1320

augcucaucc cggagcugcu gaacacgagc gaggagcaga ugaccauccu cacccggaug     1380

ggccugauga aguccgacaa cgagaaguac cgcgggcuga agaagauccc cgccgagaag     1440

aucgccagcg agaucuucaa ccccgucgug gccaagucca cccgggaggc caucaaggug     1500

uucaacgcga ucagcaagga gaucggcaag aacaacaucg agcacgucgu gaucgagcuc     1560

ccccgcgacg acaacgagga ggacgagaag cggaagaucc agaagcugca gaaggccaac     1620

gagacggaga aggaggccgc cgacaaccug gucaagcaca agcucggcau cuccgacagc     1680

gcccugaucg cgcacuaccg caaguucaag gggcuguccc agaaggugcg guacugguac     1740

cagcaggaga ccuacugccc guacugcggc aagaucaucg aggccaccca gcugaucaac     1800

aacaacgaca acuucgaggu ggaccacauc auccccauca gcgucuccua cgacgacagc     1860

cagaacaaca aggugcucgu gcacucccag ugcaaccagg ccaaggagaa gcagacgccc     1920

cuggggugga ucaacaacgg cgggggcuuc ggccagagca agaacgagua caccaaccgc     1980

gucaagaaca acaagaagua cagcaagaac aagguggaca accugcucaa cgagaccgac     2040

cugaccgaca ucaucaccca gcgguccuuc auccagcgca accugaacga cacgcgguac     2100

gccgcgcgca ccgugcucga cgaguucuac agcuucuuca aguccaacga ccugcccacc     2160

aaggucaagg uggugcgggg gaaguggacc agccagaugc gccggaagug gaacaacguc     2220

ggcgggcugc gcaagacccg ggacacgcac caccaccacg ccaucgacgc cuccaucauc     2280

gccagcuucc cgcuccugcg cgccuucgac cgcgcgauca agcugaucga cguggacaag     2340

gagaccggcg agaucgugca guacggcaag gccucccuca acggggacgu caucgaggag     2400

aaguccguga uccaggcccc caucaucaag aacgagaagu ucgagaagcu gguggacgag     2460

aucuacaacu ucccccuguu caagcagguc gagcucgcca acaacaucga gaaccccgag     2520

aacccgguga aguucagcca ccacguggac aagaaggcca accggaccgu cgcgaaccag     2580

accaucuaca gcacgcgcau caagaagacc gagaagaucg ugcggaagaa ggugaccgcc     2640

aaggaggagg aguacgucau cggcaccauc aaggacaucu acgacgugac gaacuucgag     2700

aaguucaaga agcuguacga cgaguccagc aagaaggacg acaucaaguu ccugaugcag     2760

aagaacgacc ccaagaccug ggagaagcuc guggagauca ugaacaccua ccccgacuac     2820

gaggagaucu cccagcugga cgggaagguc aagcgcguga aggugagccc cuucaagcug     2880

uacuuccggg agaacggcuu caucaccaag uacgccaagc acaacaacgg gccgaagguc     2940

guguccauca aguacuacga cagcaagcuc ggcaaccaca ucgacaucac gcccgugaac     3000

gccaacaaca aggucauccu gcagucccug aagcccuggc gcaccgacgu guacuacaac     3060

aacgagaccc agcaguacga gcuccugggc cugaaguaca gcgaccucaa guucaccaag     3120

gggguguacg gcaucacgcg ggaggccuac gagaagcuga aguacgggua caccgacggc     3180

guccccgcgu ggaagccgau cagcaaggag uccgaguuca aguucagccu guaccgcaac     3240

gaccgggugc gcaucaucaa cgaggccggg gagaagaucg accuccuguu cggcucccgg     3300

aacgagacca gcgagggcua cguggagaug aagcccaucu ucaagaccaa cuucgacgac     3360

gccgaggagg ccuccuucua cgggaagacg agcaacaagc gccugaucaa gaagauggcg     3420

cggaagggcu accagaucua caaggucaac accgacaucc ucgggaaccu guacuacacc     3480

gccaaggagu ccaacgagcc caagcuggac cucuga                               3516


<210>  1706
<211>  3519
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1706
augcgcaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gccgagcaag aagaugcgga uccaggggac gaccgaccgc      120

accuccauca agaagaaccu caucggcgcc cugcuguucg acaacgggga gaccgcggag      180

gccacgcggc ucaagcgcac cacccggcgc cgguacaccc gccggaagua ccgcaucaag      240

gagcugcaga agaucuucag cuccgagaug aacgagcugg acaucgccuu cuucccccgg      300

cucagcgagu ccuuccuggu gagcgacgac aaggaguucg agaaccaccc caucuucggc      360

aaccugaagg acgagaucac guaccacaac gacuacccca ccaucuacca ccuccgccag      420

acccuggccg acuccgacca gaaggccgac cugcggcuca ucuaccuggc gcuggcccac      480

aucaucaagu uccgcggcca cuuccucauc gaggggaacc uggacagcga gaacaccgac      540

guccacgugc uguuccucaa ccuggugaac aucuacaaca accuguucga ggaggacauc      600

gucgagacgg ccuccaucga cgccgagaag auccucacca gcaagaccuc caagagccgg      660

cgccuggaga accugaucgc ggagaucccg aaccagaagc ggaacaugcu guucggcaac      720

cugguguccc uggcccucgg gcugaccccc aacuucaaga cgaacuucga gcugcucgag      780

gacgccaagc ugcagaucag caaggacucc uacgaggagg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc aucgcggcca agaagcucag cgacgccauc      900

cugcuguccg acaucaucac cgugaagggg gccagcacca aggcgccccu cagcgccucc      960

augguccagc gcuacgagga gcaccagcag gaccuggccc ugcucaagaa ccuggugaag     1020

aagcagaucc ccgagaagua caaggagauc uucgacaaca aggagaagaa cggcuacgcc     1080

ggcuacaucg acgggaagac cagccaggag gaguucuaca aguacaucaa gccgauccug     1140

cucaagcugg acggcacgga gaagcugauc uccaagcucg agcgggagga cuuccugcgc     1200

aagcagcgga ccuucgacaa cgggagcauc ccccaccaga uccaccugaa cgagcugaag     1260

gccaucaucc gccgccagga gaaguucuac cccuuccuca aggagaacca gaagaagauc     1320

gagaagcugu ucaccuucaa gauccccuac uacgugggcc cgcuggccaa cggccagucc     1380

agcuucgcgu ggcucaagcg gcaguccaac gagagcauca cccccuggaa cuucgaggag     1440

gucguggacc aggaggccuc cgcccgcgcc uucaucgagc ggaugacgaa cuucgacacc     1500

uaccugcccg aggagaaggu gcugcccaag cacagcccgc ucuacgagau guucaugguc     1560

uacaacgagc ugaccaaggu gaaguaccag accgagggga ugaagcgccc cguguuccug     1620

uccagcgagg acaaggagga gaucgucaac cuccuguuca agaaggagcg gaaggugacg     1680

gugaagcagc ugaaggagga guacuucucc aagaugaagu gcuuccacac cgucaccauc     1740

cucggcgugg aggaccgcuu caacgccagc cuggggaccu accacgaccu gcucaagauc     1800

uucaaggaca aggcguuccu ggacgacgag gccaaccagg acauccugga ggagaucgug     1860

uggacgcuca cccuguucga ggaccaggcc augaucgagc ggcgccuggu caaguacgcc     1920

gacguguucg agaaguccgu ccucaagaag cugaagaagc ggcacuacac cggcuggggg     1980

cgccugagcc agaagcucau caacggcauc aaggacaagc agaccggcaa gacgauccug     2040

ggguuccuga aggacgacgg cguggcgaac cggaacuuca ugcagcucau caacgacucc     2100

agccuggacu ucgccaagau caucaagaac gagcaggaga agaccaucaa gaacgagucc     2160

cuggaggaga ccaucgccaa ccucgccggg agccccgcca ucaagaaggg cauccugcag     2220

agcaucaaga ucguggacga gaucgucaag aucauggggc agaaccccga caacaucgug     2280

aucgagaugg cgcgcgagaa ccaguccacc augcagggca ucaagaacag ccggcagcgc     2340

cugcggaagc ucgaggaggu gcacaagaac accggcucca agauccugaa ggaguacaac     2400

gucagcaaca cgcagcugca guccgaccgc cucuaccugu accugcucca ggacgggaag     2460

gacauguaca ccggcaagga gcuggacuac gacaaccuga gccaguacga caucgaccac     2520

aucaucccgc aguccuucau caaggacaac agcaucgaca acaccgugcu caccacgcag     2580

gccuccaacc ggggcaagag cgacaacgug cccaacaucg agaccgucaa caagaugaag     2640

uccuucuggu acaagcagcu caagagcggg gccaucuccc agcgcaaguu cgaccaccug     2700

accaaggccg agcgcggggc ccucagcgac uucgacaagg cgggcuucau caagcgccag     2760

cugguggaga cccgccagau cacgaagcac guggcccaga uccuggacuc ccgguucaac     2820

agcaaccuca ccgaggacuc caagagcaac cgcaacguca agaucaucac ccucaagagc     2880

aagauggugu ccgacuuccg gaaggacuuc ggcuucuaca agcugcgcga ggugaacgac     2940

uaccaccacg cccaggacgc cuaccugaac gccgucgugg ggaccgcgcu gcugaagaag     3000

uaccccaagc ucgaggccga guucguguac ggcgacuaca agcacuacga ccuggccaag     3060

cugaugaucc agcccgacag cucccucggg aaggccacga cccggauguu cuucuacagc     3120

aaccugauga acuucuucaa gaaggagauc aagcuggcgg acgacaccau cuucacccgc     3180

ccgcagaucg aggucaacac ggagaccggc gagaucgugu gggacaaggu gaaggacaug     3240

cagaccaucc ggaaggucau guccuacccc caggugaaca ucgugaugaa gaccgagguc     3300

cagacgggcg gguucagcaa ggaguccauc uggcccaagg gcgacagcga caagcucauc     3360

gcccgcaaga aguccuggga ccccaagaag uacgggggcu ucgacagccc gaucaucgcc     3420

uacuccgugc ugguggucgc caagaacugc aagcggaaga acagcaagac caagaacuac     3480

cagcggaccu gcgggaacaa gaacaacggc acccgguga                            3519


<210>  1707
<211>  3522
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1707
augcgcccca ucgagccgug gauccugggc cucgacaucg ggaccgacag ccugggcugg       60

gccguguucu ccugcgagga gaaggggccg cccaccgcca aggagcugcu cggcgggggc      120

guccggcugu ucgacagcgg ccgcgacgcg aaggaccaca cgucccggca ggccgagcgc      180

ggggccuucc ggcgcgcccg gcgccagacc cggaccuggc ccuggcgccg ggaccgccug      240

aucgcccucu uccaggcggc cggccugacc ccgcccgccg ccgagacgcg gcagaucgcg      300

cuggcccucc gccgggaggc cgugagccgc ccccuggccc ccgacgcccu gugggcggcc      360

cuccugcacc uggcccacca ccggggguuc cgcuccaacc ggaucgacaa gcgcgagcgg      420

gccgccgcga aggcccucgc caaggccaag ccggccaagg cgaccgccaa ggccaccgcc      480

cccgccaagg aggcggacga cgaggccggc uucugggagg gggccgaggc cgcgcugcgc      540

cagcgcaugg ccgccagcgg cgcccccacc gugggcgccc ugcucgcgga cgaccuggac      600

cgggggcagc ccguccgcau gcgguacaac caguccgacc gcgacggcgu gguggccccg      660

acgcgggccc ugaucgccga ggagcucgcc gagaucgucg cgcgccagag cuccgccuac      720

cccgggcugg acuggcccgc cgugacccgg cuggugcucg accagcgccc ccugcggagc      780

aagggcgccg gcccgugcgc cuuccugccc ggggaggacc gcgcgcuccg ggcccugccc      840

accguccagg acuucaucau ccgccagacc cuggccaacc uccggcugcc cuccacgagc      900

gccgacgagc cgcgcccccu gaccgacgag gagcacgcga aggcccucgc ccugcugucc      960

accgcccggu ucguggagug gcccgcccuc cgccgggcgc ugggccugaa gcgcggggug     1020

aaguucaccg ccgagacgga gcggaacggc gccaagcagg ccgcccgcgg gaccgcgggc     1080

aaccucaccg aggccauccu ggcgccccug aucccgggcu ggagcgggug ggaccucgac     1140

cgcaaggacc gggucuucuc cgaccugugg gccgcccgcc aggaccggag cgcccugcuc     1200

gcccugaucg gcgacccccg cgggcccacc cgggugacgg aggacgagac cgccgaggcc     1260

guggcggacg ccauccagau cguccugccc accggccgcg ccucccucag cgccaaggcc     1320

gcgcgggcca ucgcccaggc cauggccccg gggaucggcu acgacgaggc ggugacccug     1380

gcccugggcc ugcaccacuc ccaccgcccc cggcaggagc gccucgcccg gcugcccuac     1440

uacgccgccg cgcugccgga cguggggcuc gacggcgacc ccgucgggcc cccgcccgcc     1500

gaggacgacg gcgccgccgc cgaggcguac uacgggcgca ucggcaacau cagcgugcac     1560

aucgcccuga acgagacgcg gaagaucgug aacgcccugc uccaccgcca cggccccauc     1620

cugcggcugg ucauggugga gaccacccgc gagcucaagg ccggggcgga cgagcggaag     1680

cgcaugaucg ccgagcaggc cgagcgggag cgcgagaacg ccgagaucga cguggagcug     1740

cgcaagagcg accgguggau ggccaacgcg cgcgagcggc gccagcgggu ccgccuggcc     1800

cggcgccaga acaaccucug ccccuacacc uccacgccca ucggccacgc cgaccugcug     1860

ggggacgccu acgacaucga ccacgugauc ccccucgccc ggggcggccg cgacagccug     1920

gacaacaugg uccugugcca guccgacgcg aacaagacca agggggacaa gacccccugg     1980

gaggccuucc acgacaagcc cggcuggauc gcccagcggg acgacuuccu cgcccgccug     2040

gacccccaga ccgccaaggc gcuggccugg cgguucgccg acgacgccgg ggagcgcgug     2100

gcgcgcaaga gcgccgagga cgaggaccag ggcuuccucc cgcggcagcu gacggacacc     2160

ggguacaucg cccggguggc ccugcgcuac cucucccugg ucaccaacga gcccaacgcc     2220

gugguggcga ccaacggccg gcugacgggc cuccugcgcc uggccuggga caucaccccc     2280

gggcccgccc cgcgggaccu ccugcccacg ccccgcgacg cccugcgcga cgacaccgcc     2340

gcgcggcgcu uccucgacgg ccugacgccc ccgccccugg ccaaggccgu cgagggggcc     2400

gugcaggccc ggcucgcggc ccugggccgc agccgggugg ccgacgccgg gcuggcggac     2460

gcccucggcc ugacccuggc cucccucggc gggggcggga agaaccgcgc cgaccaccgg     2520

caccacuuca ucgacgccgc gaugaucgcc gucaccaccc gcggccugau caaccagauc     2580

aaccaggcca gcggggccgg ccggauccug gaccuccgca aguggccccg gaccaacuuc     2640

gagcccccgu accccaccuu ccgcgccgag gugaugaagc agugggacca cauccacccc     2700

uccauccggc cggcgcaccg cgacggcggg agccugcacg ccgccaccgu guucggcguc     2760

cggaaccgcc ccgacgcccg ggugcuggug cagcgcaagc ccgucgagaa gcuguuccuc     2820

gacgccaacg cgaagccccu gccggccgac aagaucgccg agaucaucga cggguucgcc     2880

agcccccgga uggccaagcg cuucaaggcg cuccuggccc gguaccaggc cgcccacccc     2940

gaggugcccc cggcgcuggc cgcccucgcc guggcccgcg accccgcguu cggcccccgc     3000

gggaugaccg ccaacaccgu caucgccggc cgguccgacg gcgacgggga ggacgccggc     3060

cugaucacgc ccuuccgcgc caacccgaag gcggccgugc ggaccauggg gaacgccgug     3120

uacgaggucu gggagaucca ggugaagggc cgcccccggu ggacccaccg cgugcugacc     3180

cgguucgacc gcacgcagcc cgcgcccccg cccccgcccg agaacgcccg gcucgucaug     3240

cgccugcggc gcggcgaccu gguguacugg ccccucgagu ccggggaccg gcuguuccug     3300

gugaagaaga uggccgucga cggccgccuc gcccuguggc ccgcccggcu ggccaccggg     3360

aaggcgaccg cccucuacgc ccagcugagc ugccccaaca ucaaccugaa cggcgaccag     3420

ggguacugcg ugcaguccgc cgagggcauc cgcaaggaga agauccggac cacgagcugc     3480

accgcccucg gccgccugcg gcuguccaag aaggcgaccu ga                        3522


<210>  1708
<211>  3525
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1708
augggcaagu acagcaucgg gcuggacauc ggcaucucca gcguggggug guccaucauc       60

gacaucgaga agaagaagau cgucgaccuc ggcagccgca ucuuccccuc cgggaacgcc      120

gccgcgaacc aggaccggcg cggcuaccgg ggcacccgcc ggcugauccg ccggcgcaag      180

aaccggcugg ccgacgugau caacuuccuc ggggaggaga acuucaucga caccaaggug      240

gucgaccaga acggcaacau ccacuacaag cugcugguga acagcacgga guccccguac      300

aagcuccgcg ugaaggggcu gagcaagaug cugaccaagc aggagcucgu caucgcccug      360

uaccacaucg ugaagcaccg gggcaucucc uacgaccugg gggaccucga ggacgacggc      420

accagcggcg ugaccgacua caagacgucc aucaacauca accgccagcu gcugaucaag      480

aagaccgucg ggcagaucca gcucgagcgg cugaaggagu ucggcaaggu gcgcgggcac      540

gugaagaagg acgagaacau cacccugcuc aacgucuucc ccagcuccgc cuacgugaag      600

gaggccaccc agauccugac gaagcagcgg gaguucuacc ccgagaucac cgacgacuuc      660

aucaccaagu ucauccccuu caucagccgc aagcgggacu acuucguggg cccgggcaac      720

gagaaguccc gcaccgacua cgggaucuac aagaaggacg gccggacgcu ggacaaccuc      780

uucgacgagc ugaucgggau cgacaagauc agcggcgaga agcgcgcguc cgccagcucc      840

gucaccgccc agaucuacaa caugcugaac gaccucaaca accugaccgu gaccaacacg      900

gaggacggga agcugaccac ccagaccaag cgggagauca ucgaggccga caagacgacc      960

aacggccucu ucggcaucac ccagaucugc aagaucaucg ggugcaagaa ggacgacauc     1020

aagggcuucc gcaucgacaa gguggagaag cccaucaugc acacccugag cggguaccgc     1080

cgguuccgca aggagcuggc gaagaucaac aucgaggcca acgaccucga caucaaccug     1140

gucaacaaga ucgccgacau ccugacgcuc aacaccgaga aggccgagau ccggaagcag     1200

cugaacaaga gcaacaucga cguguccaac gagaucaagg agcugaucau caacaagaac     1260

aaggacuucc ucguggacgg ccgcgccacc uggcacagcu ucuccuacaa gacccugaac     1320

cugcucaucc ccgagcugau gaacacgagc gaggagcaga ucaccauccu gacccggcuc     1380

gggcugguca aguccaacag cgagaaguac aagggccuga agaacauccc cgcgcgccgg     1440

aucaccgagg gcaucuacaa cccgguggug gccaaggcca cgcgcgaggc cgucaacgug     1500

uucaacgcca ucgcgaagaa gaucgggcgg gacaacaucu ccgacgucgu gaucgagcuc     1560

ccccgcgagg acaacgaggc cgacaucaag aagggcaucg ugaagcggca gaagcagaac     1620

gagcuggaga aggaggccgc cgacgcccug gucaagcagc agaugaccau cagcgacucc     1680

gcgcucgugg cccacuaccg caaggugcgg gggcugagcc agaagguccg cuacugguac     1740

cagcagggga ccugcugccc cuacugcggc aagaacaucg aggccgugca gcugaucaac     1800

aacaacgacu ccuucgaggu ggaccacauc auccccauca gcgucuccuu cgacgacagc     1860

cagaacaaca aggugcuggu gcacucccag ugcaaccagg ccaagggcaa gcagaccccg     1920

cucggcuggc ugaacaacgg gggcggguuc ggccagaaca agacggagua cgucgccaag     1980

gugaagacca acaagaacua cagcaagaac aagauggaca accugcucaa cgacauggac     2040

cuguccgaca ucgugaccca gcggggcuuc auccagcgca accugaacga cacccgguac     2100

gcgagccgca ucguccucga cgaguucuac uccuucuuca agagcaacaa ccugagcacc     2160

aaggugaaga ucgugcgggg gaaguggacg ucccagaugc gcaagaagug gaacggcguc     2220

gggggccuga agaagacccg cgacaccuac caccaccacg ccaucgacgc cagcaucauc     2280

gccuccuucc cccuccugaa gguguucgac aaggcgauca agcugaucga caucgaccgg     2340

gagaccgggg agauccucca ggacaaggag gccaucaaga ugagccagga ggccaagcug     2400

cuggagaaga uccccgugau ccagaacgag aaguucgagg agcucaucaa cgagcuguac     2460

gacuuccccc uguucaagca ggucgagcuc gccaacgaca ucacgaaccc ggagaacccc     2520

gugaaguucu cccaccacgu ggacaagaag gccaaccgca gcgucgcgaa ccagaccauc     2580

uacggcaccc ggaucaagga gaaggagauc aacaagcgcg gcaaggugga gaaggugcag     2640

gagcgguaca uccuggggac caucaagaac aucuacgaca ucgacuccuu cacgagcuuc     2700

aagaagaucu acgacaagau guccaagacc ggcgacguca aguuccugau gcagaagaag     2760

gaccccaaga ccugggggaa gcucguggag auccugaaca gcuaccccga cuccgaggag     2820

aucacccagg uggacggcaa ggucaagcgc gugaagguga gcccguucga gcuguaccgg     2880

cgcgagaacg acuucaucac gaaguacgcc aagcacaacg acgggcccaa gaucgucucc     2940

aucaaguacu acgacagcaa gaucggcucc cacaucgaca ucacccccau caacgccaag     3000

aacaaggugg ugcuccagag ccugaagccc uggcggaccg acgucuacuu caacuccgag     3060

acccgccagu acgagcugcu cggccugaag uacagcgacc ugaaguucac gaacggggug     3120

uacggcauca ccagcgaggc cuacgagaag cucaagcacg ggaucuccga ggacggcacc     3180

aagaccugga agccgaucgg gaagaacagc ggcuuccggu ucucccugua ccgcaacaac     3240

cgggugaaga ucgucgacga gaagggcgag agccuggagc uccuguucgc cucccgcacg     3300

accagcaacg agggguacgu ggagcugaag cccaucuaca aguccaaguu cgacagcaag     3360

gaggacgugg gcuucuacgg gcagaugacc cccuccggcc aguucgucaa gaagauggcg     3420

cggguggggu acaagcucua caaggugaac accgacgucc ugggcaaccc cuacuacauc     3480

acgaaggaga gcgacgaccc ggagcugucc cucuacaaga aguga                     3525


<210>  1709
<211>  3528
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1709
auggaggaga ugaagcagac cggccgcaag aacgaggccc ccaagcugac cgaccucaac       60

uaccggaucg ggcuggacau cggcaucgcc agcguggggu gggcgguccu ggagaacaac      120

ucccacgacg agccguuccg caucaucgac cucggcgugc ggaucuucga cacggccgag      180

cagcccaaga ccggggccag ccuggcccug ccccgccggg aggcccgcuc cgcgcggcgc      240

cggauccgcc ggcgccggga ccgcaucgag cggaucaaga agcuguucca ggccgagggc      300

cugaucaaca ucgacgaguu cgaggcccgc uacaagaucg ccggccugag cgacguguac      360

aagcuccggu acgaggggcu ggaccgcaag cugaccgacg acgagcucgc gcagguccug      420

cugcacaucg ccaagcaccg gggcuucaag uccaccagca aggccgagga ggccgagaac      480

gagaacgacg ccgcgaagaa gaaggaggcc ggggcccuca agaagggcau cuccaacaac      540

aagaagcuga uggaggagaa gggguaccgc acggugggcg agaugaugua ccuggacgag      600

aacuuccgga ccgacugccc cuggaacgag aacggcuaca uccucacccc gcgcaacaag      660

agcggggacu acaagcacac cgugcugcgc gacaugcugg ucgacgaggu gcgggagauc      720

uucaaguccc agcgcagccu cggcaacauc cacgccuccg aggagcugga gaacgccuac      780

cuggacauca ugcucagcca gcgguccuuc gacauggggc ccggcgcgcc cagcaaguac      840

gccauggagg gcuucgagga ccgcgugggg cugugcacgu ucgagggcaa gaacggggag      900

aagcgggccg ccaaggccgc guacaccucc gagcuguucg ucgcccucga gaagaucaac      960

cacaccaaga ucgugagcga cgacggcgcc acccgcuucc ugacgaccua cgagcggaag     1020

uccaucaucg agcugaugca caagcagcag accgugaagu acgccgccgu ccgcaaggcg     1080

aucgggcucg ccgaggagga caaguucuac aaccugaacu acagccccaa ggccggcucc     1140

aagaaguccc cggaggacac cgacuucgug aagcuggaga acuaccacaa gauccggaag     1200

gcccucggcg aggacaucgu gagcgagcac cuccccgcgg agaagaucaa gcuguacgac     1260

agcaucgcca cgauccucac ccuguucaag aacgacgaca gccgcaucaa gcgguucauc     1320

gagcugggga ucggcgagga gugcuucgac gggcuccugu cccugagccc cuccaaguuc     1380

cacaaccuca gccuggucgc caugggcaag aucauccccu uccugcucga cgggaacacc     1440

uacgacaagg ccugcgagcu ggccggcuac gacuucaagg cggacaacaa gggcgagaag     1500

uccgugcugc ucaaggggaa gaacaucacc gacaugguga acgacaucac gaacccgguc     1560

gugcgccgga gcguguccca gaccaucaag gucaucaacg ccaucauccu ggaguacggc     1620

agcccccagg ccgugaacau cgagcuggcc cgcgagaugu ccaagaacuu cgacgagcgg     1680

cgcaagcuca agagcgacau ggagucccgg gagaaggaga acaacaacgu gaucaagcgc     1740

gucaaggagu acaagcugaa ccccaccggg caggacaucg ugaaguuccg gcugugggag     1800

gagcaggacc gccgcugccu guacagcggc gagaagaucg acaucaacga ccucuucucc     1860

cgggacgggg gcuacgacgu cgaccacauc cugcccuaca gcaucaccuu cgacgacacg     1920

uaccgcaaca aggugcuggu gaaggccucc gagaaccggc agaagggcaa ccgcaccccg     1980

uacgaguacu ucauggcgga gaaggacgac gacgagugga acgucuacga gacccucgug     2040

cggaacaccg ugaaggacua caagaagguc cugaagcugc ucaagaagca caucacggag     2100

gacgagaagu ccgaguuccg cgagcggaac cugaccgaca ccaaguacau cacccgcauc     2160

aucuacaaca ugauccggca gaaccuggug cucaagcccu accugagccu ggagaaggag     2220

cgcaagaagc aggucauggc cgugaacggg agcaucacgc acuaccucag caagcggugg     2280

ggccuggagu ccaaggaccg ccggaccgac acccaccacg cccaggacgc cguggugauc     2340

gccugcacca cggacgggau gaucaacaag aucagccgcu acuccaaggg ccgggagcug     2400

cucuacagcc gcggcuucaa guucgucgac gaggagaccg gggagauccu gucccgggac     2460

aacuucaccc gcgaggagug ggacgagaag uacggcguga agauccccau ccccugggag     2520

cacuuccggg acgagcugga cauccgcaug agccgguaca acgggaccgg cgacgacucc     2580

aucgacaaga ccacgccgcg cgacuucauc cucagccacc gcgacgcgca caaguggauc     2640

aacuaccccg aguggauguu cguggagggg cggcccggcc gccggggcgu ccuggacggg     2700

aucuucgugu cccgcaugcc caagcacaag gugagcggcc aggcccacga ggagaccguc     2760

cgguccgcca aguacuacaa cgacgggggc agcguguucg aguccggggg cuacgugguc     2820

accaagaagu ccauccagga caucaagcug aaggacggcg agaucaagga guucuucaac     2880

ccggacagcg accgccuccu guacaacgcc cugguggacc ggcucaacaa guucgagaac     2940

gaccccaaga aggcguucgc cgagcccuuc uacaagccca aggccgacgg gacccagggc     3000

ccgaucguga agaagaucaa ggucuucgag aagcagagcu ccgggguguu cgugcgcgac     3060

ggcaagggga ucgccgccaa cggcgcgaug guccggaucg acguguucug cgaggaggac     3120

aagggcaaga agaaguacua cuucgugccc aucuacacgg ccgacgucgu gaagaagaag     3180

cugcccgacc gcgccgccac cgccaacaag cugaucagcg aguggcgggu gaugaaggag     3240

gagaacuuca ucuucagccu cuacucccgc gaccugaucu acaucgagag cgacaagggg     3300

guccagguga aggacaucga cggcaagauc uccaagccgg ugaaccuguu cgcguacuac     3360

cucggggccg acaccagcac cgccuccauc agcggcaagc ugcacgacuc cagcgccaac     3420

uuccggucca ucgggaucca gggccugcgc cgguucgaca agugccaggu cgacgugcuc     3480

ggcaacauca gcuucgugaa caaggagaag cgcaugcccu ucaacuga                  3528


<210>  1710
<211>  3531
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1710
augaacaagg ugcaggagga cugcuacaag aagaucaagg agaagcuggc cgaccgcgag       60

uaccggaucg gccucgaccu gggggucggc agcaucgggu acgccguggu gucccugaag      120

ggcgacaugc ccgaggacgu cauccucgcg gggagccgca ucuucaaguc cagcaucggc      180

gccgccgagc ggcgccacuu ccgggcccag cgcaacaccc accggcacca ccgcgagcgg      240

augcgcuacc uguggaagcu gcucgccgag aagaaccugg cgcugcagcu cccggagaac      300

cuguccaaga aggagaacuu caccgacuuc gacacgagcc ggaagcgcuu ccccaucgag      360

gugcugcgga acgaccccua cauccuccgc uacaaggccc uggacgagaa gcugucccuc      420

uuccagcugg gcuacgcccu guaccacauc gccaaccacc gggggaccgc gagcgugcgc      480

uccuuccucg aggacgacga ggccaagguc aaggagaaca aggagaccgc caagcuggcc      540

aagaacacca agaagaucau guacgagaag aaguaccgga cguucggcga guaccuguac      600

cgcgagcgga ucgagaaguu ccgccagaac gagcggauga aggugaccaa caagaacgag      660

aacaaggagu ucgccgugac ccgcgacauc aucgagaagg aggucgagau gauccucggg      720

gcgcagaagc gcuacaacac cgccgugcug gccgacgacu acaucgagcg gaucaagaag      780

gccaucuucu acgagacgga gaagcugauc cccgagccgg aguacugccc cuacuucaag      840

gacgacaagc gccucccccg gagccacaag cugaacgagu accgccggau cuacgaggcc      900

cugaacaaca cccgcuacua cauccccgag aucgaggaga agaccggcga gauccucucc      960

cggaaggaga aggagcagcu gagcaaggag gagaaggaga agcuguucaa cuuccuccug     1020

aagaacaagg agcugaccga ggcgaaggug aagaagcucc ugaaccuguc cgagauggcc     1080

aagguccagc uccaggggcg cgacaagaag aaccagaaga ucaagggcua cgcccugauc     1140

acgcuggaga gccucccguu cugguccaag cugcccgagc agcagcagga cgaguuccug     1200

uacgacugga acaagcuccc cgacgagaag cugaaggacc ggcugcgcaa cuacuacaag     1260

cucaccgacg acgagaucga cgagaccuuc cagaacaucg ugcugcccag caacuacgcc     1320

ccgcugggca agaaggccau ggagauccuc cuggaguaca ucaagaacgg gcuguccuac     1380

aucgaggcgc uggacaaggc cgccgacgag aacaagauca agguggagaa gcacgagauc     1440

aacgaccucc ugcccuacua cggcaagauc cugcaggaga gcacccagau gcccaucggg     1500

aaggccuucu ccaagcaguu cgccgcgaag aacuacaaga agcccgacau caacaagaac     1560

gagcaggagu ucggccggau cgccaacccg gucgugcacc agacgcucaa cgagcugcgc     1620

aagcugauca acgagaucau caccguguuc ggcaagaagc cccucgagau cagccuggag     1680

accagccggg agcugaagaa gucccagaag gagcgcgagc ucaucgccaa gcagcaggac     1740

aucgacgagg agaagcggaa gaagaucugg aagaccuaca ucgagcccca ccuccagcag     1800

aucaagagcc gcuccgacaa gcugaagaag cuguucgucu ucgagcugug ggaggaccag     1860

aagcacaacu gccccuucug ccuggagaag aucaacgugg acgacaucua cagccgcggg     1920

guggacaagg agcaccucuu cccgcugaag aaguccgagg acgacagcga gaacaaccug     1980

gucaucgccc acaacaagug caacgcgcgc aaggccgagc gggcccccuu cgacgccuuc     2040

ggccacgagu ccaucuggaa cgacaucuuc agcaacgccg agaagaaccu cccccgcaag     2100

cuguggcgcu uccggcaggg guccuucgag aaguuccugg agaagccccc gaugcucaag     2160

cgcuucgaga cggacaacag cuacaucucc aagaucagcg cgcgguaccu ggccugccug     2220

uucgacaagc ccuccaagau cuucugcgug aagcccuucc ucaccgccca gcugcgcauc     2280

gccuggcagc uggacgagau caugauccgg cucgccgaga acaucauccu ggacagcgag     2340

aaggacuccu ucaacaaggg cgggcugcag aagaagaagc gcaucgacaa ccggcaccac     2400

gcgcucgacg ccguggucau cgccuacgcc agccgcggcu accacaacuu ccugaaccgg     2460

gugcacgcca agggcuacaa gaucaacuac gacgagaaga ccuggcuguc cgagacccuc     2520

cggcccccgg ggaacaagaa ccuggaggac uucaagagcg gcaucaucaa cgucaugauc     2580

aacgcgaaca ucuccaucaa gcacgaccac aacaacaagg ggcagcuggu caagagcaag     2640

gccuacaagc ucuacuacag cgagaucggc aaguacauca ucacgacccu gaaguccgug     2700

agcgagaucc uguucaagca gaaggagaag ccccaggaca cccuccggcg cgcccugugc     2760

aaguucgagg accggcuguc cgacaucaag gacaagaagc uccgcaagaa gcuggaggac     2820

aacaucacca ucuacaagag caucgagaac gaguccgccc ugagcaaggc gaagcaggag     2880

cucgaggagu ucgaccugaa ggccaagaag gaggggaaga agggcuacga gaagaucacg     2940

gacauccuga ucuacaagaa ggccugcucc augcacggcg ggaaguacau ccacgugaag     3000

aaccggaucg agaacaaguu cuucgcccuc aaggagccca ccgagaucga gaccggcuuc     3060

ggguacgaca ccgccgacaa ccugagccug gaccucuacc acgacaacaa cggcaagcug     3120

ugcggggagg ugauccgcaa gaucgacuuc aaccggaacc gcgugccgaa cuacaagaag     3180

gagggcuaca uccugcucga gcggaucuac cagggcgaca cgcuggaggu cgacaucucc     3240

gacgacaaga ucagccugaa gaacaaggug ggguccgcgc ccgacaagcg caccuucgug     3300

aagguccuca ccuucaccga gagcgaccgg uaccugaaga acaagcgcga ccagauccag     3360

aucuucuucu gcaaccugcu caaguccacg cugaagcacg acgacagcuc caagcacgac     3420

gacagcuucu acguguccag caugcagaag uacaacgugc gcaaggucau ccugaccagc     3480

cucggcauca ucaaguaccg guccaagauc cugaagaaca aggagaucug a              3531


<210>  1711
<211>  3531
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1711
augcuguucc ccaagaagcu cagcaaggag gagguggccu acguccuggu ggagaucaac       60

uccaaccuga acaacagcuc caacuaccuc ggcaacauca gcgaccgcuc caaggagcug      120

uacuucaaca agcagaccau cgggcaguuc cuguacacca agcucaagga ggaccggcac      180

uacagccuga agaacaaggu guucuaccgc caggacuacc uggacgaguu cgagcggauc      240

ugggagacgc aggccaucuu ccacaaggag cucaccgagg accugaagaa gcagauccgc      300

gacaccauca ucuucuacca gcggcgccug aagucccaga agggccucau cagccugugc      360

gaguucgaga acaaggagau caccaucgag aaggagggga agacggucac caagcagauc      420

ggcgugcggg ugaucccgaa guccagcccc cuguuccagg aguucaagau cuggcagauc      480

cucaacaacg ucaucugcaa gaagaagggg acccgcaaga agaaggcggu gacccccgcg      540

ccccugcugu ucgagcuguu cggcacgccg uccgagaccu ucuucagccu ggacauggag      600

ugcaagcagc uccucuucga ggagcugaac cugaagggca accucaaggc cgacaaggug      660

auccggcugc ugggguacau cccccaggag ugggagauca acuacaacca gcucgagggc      720

aaccgcaccc agaagucccu guacgaggcc uaccugaaga ucaucgagau cgagggguac      780

gacaucaaga ccuuccuccg gaucaagagc gacaaggagg acugggaccu ggaggagagc      840

ccccuguccg ccaaggagau ccgcgagaac auccagcaca ucuucgagac gcucggcauc      900

uccaccgcca uccuguacuu cgacgccacc cugcccauca aggacuucga gaagcaggcc      960

agcuaccagc ucuggcaccu ccuguacucc uacgagagcg acaacagccc guccgggaac     1020

gagacccugu acaagcugcu gcaggacaag uucggcuuca agcgggagca cgccgucuuc     1080

cucagcaaca ucuccuucca ggacgacuac ggcagccugu ccgcgaaggc cauccgcaag     1140

aucuaccccu acaucaagga gaaccaguac agcacggccu gcgagcuggc cggguaccgg     1200

cacuccaagc ucagccugac caaggaggag cugaacaacc gcccccucaa ggaccugauc     1260

agcccccuga agaagaacuc ccugcggaac ccgguggugg agaagggccu caaccagaug     1320

aucaacgucg ugaacgcccu gaucgagaag aacuccgaga aggacgcgga ggggaacauc     1380

aucaaguacu uccacuucga cgagauccgc aucgagcugg cccgggagcu caagaagaac     1440

gcccaggagc gcgccgagau gacccugaac aucaagaacg ccaccaucga gcaggagaag     1500

aucaagagca uccugaucuc cgacuucggc aucaagaacc ccacgaagaa cgacaucacc     1560

cgguaccgcc ucuaccagga gcuggcgcac aacgacuaca aggaccugua caccaacacc     1620

gacaucccca aggagcuccu guucagcaag aacaucgaca ucgagcacau cgugcccaag     1680

gcccgcgucu ucgacgacuc cuucagcaac aagacgcugg ccuaccggga cuucaaccuc     1740

aagaagggcg agaacaccgc cuucgacuuc aucgacucca gccuggggga ggaggagcug     1800

aaccaguaca ucgcccgcgu ggagaacgcg uucaagacca agaacaucuc cgccaccaag     1860

uaccggaacc uccugaagaa ggccacggag aucaacgacg gcuucaucaa ccgcgaccug     1920

cgggagaccc aguacaucgc caagaaggcc aaggaccucc uguuccagau cacccgcagc     1980

guggucagca ccuccgggag caucacggac cggcugcgcg aggacugggg ccucaucgac     2040

aucaugaagg agcugaacau gccgaaguac cgggaccugg ggcucaccga gaucaucgag     2100

cgccgggacg gccgcaaggu ggaggugauc aaggacugga ccaagcggaa cgaccaccgc     2160

caccacgcga uggacgcccu gaccgucgcc uucacgaagc ccuccuacau ccaguaccug     2220

aaccaccuca acgcccggaa ggacgagaac aacaagcugu acgcggacau ccugggcauc     2280

gagcgcaagg agaccaccaa gauccacgac gagaacggga acgagaagcg gguguucauc     2340

gagcccaucc ccggcuuccg ccagaucgcc aagaaguacc ucgaggagau ccugaucagc     2400

cacaagacca agaacaaggu ggucaccaag uccaucaaca agaucgccgg gacgaacaag     2460

aagcaggaga cccugacccc gcggggccag cuccacaagg agaccaucua cgggaaguac     2520

cacuacuaca gcaacaagga ggagaagauc ggcacgaagu ucgacaagga gaccaucucc     2580

aaggugagca accccaucua ccgcuacgcc cugcagaagc gccuggaggc caacgagaac     2640

aaccccaaga aggcguucac cggcaagaac gcccucucca agaaccccau cuaccuggac     2700

gacgccaaga ccaagauccu gccggagaag gucaaccuca ucuaccugga ggaggacuuc     2760

agcauccgga agaagaucgu guccgagaac cccacgagca aggaguucgu gcccaacucc     2820

aucagcaucg agaagaucgu cgacgagggg aucaagcgca uccugaagga gcggcucgag     2880

accuacaaca acgaccccaa gaaggccuuc gccgaccugg acaagaaccc gaucuggcug     2940

aacaaggaga agggcaucuc caucaagcgc gugaccauca gcggggugaa gaacacccag     3000

gcgcuccacu ccaagaagga ccaccugggc aagcccaucc uggacaagga ggggcaggag     3060

auccccgucg acuucgugag cacgggcaac aaccaccacg uggccaucua ccgggaccag     3120

aagggcaacc uccaggagca gaucgucagc uucuucgagg ccguggugcg cgcccagcag     3180

gggcugccga ucaucgacaa gaccuacaag caggaggagg gcuggcaguu ccuguucacc     3240

augaagcaga acgagauguu cgucuucccc aacgagauca ccggguucaa ccccuccgag     3300

aucgaccucc uggaccccaa gaacaagaag cugaucagcc cgaaccucuu ccgggugcag     3360

aagaucucca ucaaggacua cuucuuccgc caccaccugg agacgaacgu ggagaccaac     3420

aacauccuga aggacaucac cuggaagcgg ggcggccuca gccugcugaa ggggaucauc     3480

aagguccgca ucaaccaccu cggcaacauc guguccaucg gggaguacug a              3531


<210>  1712
<211>  3534
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1712
augaacaagg ugcaggagau cgaguacaag aagaucaagg agaagcuggc cgaccgcgag       60

uaccggaucg gccucgaccu gggggucggc agcaucgggu acgccguggu gucccugaag      120

aaguacgacg accucagcua ccugcccgag gacaucaucc uggcgggcuc ccgcaucuuc      180

gccagcucca ucggggccgu cgagcggcgc gaguuccggc uccagcgcaa cagccaccgg      240

caccgccggg agcgcaugcg guuccugugg aagcugcucg ccgagaagca gcuggcccug      300

cagccgucca aggaccucga gaagaaggag aacagcgcgg acugcgagac cucccagaag      360

cgcuucccca ucaacauccu gaagaacgac cccuacaucc ugcgguacaa ggcccucgac      420

gagaagcuga gccuguucca gcucggcuac gugcuguacc acguggccaa ccaccgcggc      480

accgacucca uccggagcuu ccuggaggac acggacgaca agaucaagga gaacaccaag      540

accgccaagc ucgcgaccga gacgaagaag aucaucgacu ccaagaagua ccgcaccuuc      600

ggggaguacc uguacaagga gcggauccag cagaacgagc gcaagaaggu cagcaaccgg      660

aagaccgccg gcaacaagaa caaggaguuc gccgugaccc gcgacaugau cuucaaggag      720

acggagaaga uccuggccuc ccagaagcag uacuacacca acgugcucac cgacgaguac      780

aucaaggaga ucgagggggc caucuucuac cagaccgaga agcugauccc cgagagcggc      840

uacugcccgu acuucaagga cgagaagcgg cugcccugcu cccacaagcu caacgaguac      900

cgccgcaucu acgaggcgcu gaacaacgcc cgguacagcg cccccguccu ggacgagaag      960

acgggggaga uccucuccua ccaggagggc gaggaguuca gcagcgagga gaagaagauc     1020

cuguucgacg aggugcugcu caagggcaag aagcugaccg aguccgagau caagaagcug     1080

cuccaccucc ccgacgggug cgagauccag cugcagggcc gcgacaagcg gacccagaag     1140

aucaaggggu acgcccugac cgcccuggag gcgcugccgu ucuggagcaa gcucucccag     1200

cagcagcagg acgaguuccu guacgacugg aacggccugc ccgaggagaa gcucaagccc     1260

aagcugaucg acugcuacag ccugacggag aacgaggugg acgagaccuu ccagaagauc     1320

cgccuccccu ccaacuacgc cccggucggc aagaaggcca uggagauccu gcugaccuac     1380

aucaaggacg gguacagcua caccgaggcc cuggacaagg ccguguccga gggcaaguuc     1440

aagguggaca agcagacgac caucuacgac agccuccccu acuacgggaa gauccugcag     1500

gaguccaccc agaagcugau cggcaaggcg uucagcaagc aguucgccaa gcggaacuac     1560

aagaagccca acaccaaccg caacgagcgg gaguucggga agaucgccaa ccccgucgug     1620

caccaguccc ucaacgagcu gcgcaagcug aucaacgaga ucaucacggu guucggcaag     1680

aagccgcucg agaucggccu ggagaccgcc cgggagcuga agaaguccag caaggaccgc     1740

gagagccucg ucaagcagca gaacgagaac gaguacaacc ggaacaagau cuacaaggac     1800

uacguggagc cccaccuguc ccagaucaag agccgccagg agaaccccuc ccgguacauc     1860

uucaaguucg agcuguucga ggagcagaag uccaucugcc ccuucugccu caaggagauc     1920

accccggacg acaucaucaa cagcaaggug gacaucgagc acaucuuccc caucgaggag     1980

agcgaggacg acgccaagaa caaccugguc aucgcgcaca acgagugcaa cgccgacaag     2040

gggaagaagu cccccuucga cgccuucggc gacaagacca gcgggaagua cguguggaac     2100

gacauccugc acaacgccaa gaagaaccuc ccccacaaga ucuggcgcuu cuaccagggc     2160

agccuggaga aguuccugga gaacaagccg augcucaagc gguucgggac ggacaacucc     2220

uacaucagca agaucgcggc caaguaccug gccugccugu ucaacaacuc cagcaagauc     2280

uucugcauca aguccagccu caccgcccag cugcgcaucg ccugggagcu gaacuccauc     2340

augauccccc ucgcgaagaa cauccugagc gagaaggagc uggaggaguu caagacccuc     2400

aagaacaaga agauccggau ggacaaccgc caccacgccc uggacgccau caccaucgcc     2460

uacgccaacc ggggcuacca caacuuccug aacaagaucc acgcgaaggg cuacaagauc     2520

gacuaccgcg agaagaacug gcucuccaag auccugcugc cgccccucaa ccgcgaccug     2580

gaggacuuca agaucagcgu gaagaacucc aucgagaagg ccaacgucag caucaagcac     2640

gaccacaaga cgaacggguc ccuggugaag ggcaccgccu acaaggucua cuacagcggg     2700

accggcaagu acaucaucac cacgcucaag aacguggagg agaucuccuu caaggagaag     2760

gaggaccccg aggacacccu gcggcgcgcc cugugcgggu ucgaggacag caacaucaag     2820

gacgagaagc ucaucaagaa gcugaaguac aacagcgacc uguacaagaa gauccucaac     2880

accaagccca aggccaagca ggagcuggag caggcgaacc aggagaccaa ggaggagggc     2940

aagaagaccc agaagaucac ggacgugcug aucuacaaga aggccugcuc ccucgucggc     3000

gagcaguaca uccagugcgc cagccggcug gagaacaagu ucuucgccgu gaaggagccc     3060

accgagaucc gcaccgggau cggcuacgac accggggaca accuguuccu cgaccuguac     3120

uacgacaaga cgcggaagcu gugcggcgag aucauccgca agaucgacuu caaccagaac     3180

aagaucccga acuaccagaa ggagggcuac auccucuucg agcggaucua ccagggggac     3240

acccuggagg cggacaccuc cgaggacaag aucagccuga agaacaagac cggcuccgcc     3300

cucgacaacc gcacguucgu gaagguccug accuucaccg agagcgacaa guacuucgac     3360

gggaagaagg acaagaucca gaucuucuuc ggcaaccugc ucaaguccaa cuacaagcag     3420

gacgacagcu ucuacaucuc cagcaugcag aaguacaacg ugcggaaggu gauccugacc     3480

ucccugggga ucaucaagua ccgcagccag auccucaaga acaaggaggu cuga           3534


<210>  1713
<211>  3540
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1713
augagcaaga aggugucccg ccgguacgag gagcaggccc aggagaucug ccagcgccug       60

ggcagccggc ccuacuccau cgggcucgac cugggcgucg ggagcaucgg cguggccgug      120

gcggccuacg acccgaucaa gaagcagccc uccgaccugg ucuucgugag cucccgcauc      180

uucaucccca gcaccggggc cgccgagcgg cgccagaagc ggggccagcg caacucccuc      240

cggcaccgcg ccaaccggcu gaaguuccug uggaagcucc uggcggagcg caaccugaug      300

cucagcuacu ccgagcagga cgugcccgac ccggcccggc ugcgcuucga ggacgccguc      360

gugcgggcca accccuacga gcugcgccuc aagggccuga acgagcagcu gacccucagc      420

gagcuggggu acgcgcugua ccacaucgcc aaccaccggg gcuccagcuc cgugcgcacg      480

uuccucgacg aggagaaguc cagcgacgac aagaagcugg aggagcagca ggccaugacc      540

gagcagcugg ccaaggagaa ggggaucagc accuucaucg agguccucac cgccuucaac      600

acgaacggcc ugaucgggua ccggaacucc gagagcguca agagcaaggg cgugcccgug      660

cccacccgcg acaucaucuc caacgagauc gacgugcugc uccagaccca gaagcaguuc      720

uaccaggaga uccugagcga cgaguacugc gaccggaucg uguccgcgau ccuguucgag      780

aacgagaaga ucgucccgga ggccggcugc ugccccuacu uccccgacga gaagaagcuc      840

ccccgcugcc acuuccugaa cgaggagcgc cggcuguggg aggccaucaa caacgcccgc      900

aucaagaugc cgaugcagga gggggccgcg aagcgguacc agagcgccuc cuucagcgac      960

gagcagcgcc acauccucuu ccacaucgcc cgguccggca ccgacaucac gcccaagcug     1020

gugcagaagg aguuccccgc ccugaagacc agcaucaucg ugcuccaggg gaaggagaag     1080

gccauccaga agaucgcggg cuuccgcuuc cggcgccugg aggagaaguc cuucuggaag     1140

cggcugagcg aggagcagaa ggacgacuuc uucuccgccu ggaccaacac ccccgacgac     1200

aagcgccuca gcaaguaccu gaugaagcac cuccugcuga cggagaacga ggucguggac     1260

gcccugaaga ccgugucccu caucggcgac uacgggccga ucggcaagac cgccacccag     1320

cugcugauga agcaccucga ggacgggcug acguacaccg aggcccugga gcggggcaug     1380

gagaccgggg aguuccagga gcucagcguc ugggagcagc agucccugcu gcccuacuac     1440

ggccagaucc ugaccggcag cacgcaggcg cucaugggga aguacuggca cuccgccuuc     1500

aaggagaagc gcgacagcga gggcuucuuc aagcccaaca ccaacuccga cgaggagaag     1560

uacgggcgga ucgccaaccc cguggugcac cagacccuga acgagcugcg caagcucaug     1620

aacgagcuga ucaccauccu gggcgccaag ccgcaggaga ucacggucga gcucgcgcgg     1680

gagcugaagg ugggggccga gaagcgcgag gacaucauca agcagcagac caagcaggag     1740

aaggaggccg uccuggccua cagcaaguac ugcgagccca acaaccucga caagcgguac     1800

aucgagcgcu uccggcugcu ggaggaccag gccuucgugu gccccuacug ccucgagcac     1860

aucagcgugg cggacaucgc cgccggccgc gccgacgucg accacaucuu cccccgcgac     1920

gacaccgccg acaacuccua cggcaacaag gugguggcgc accggcagug caacgacauc     1980

aaggggaagc gcaccccgua cgccgccuuc agcaacacgu ccgccugggg ccccaucaug     2040

cacuaccugg acgagacccc cgggaugugg cggaagcgcc ggaaguucga gaccaacgag     2100

gaggaguacg ccaaguaccu gcagagcaag ggcuucgucu cccgcuucga gagcgacaac     2160

uccuacaucg cgaaggccgc caaggaguac cuccggugcc uguucaaccc caacaacgug     2220

accgccgugg ggagccugaa gggcauggag acguccaucc uccgcaaggc guggaaccug     2280

cagggcaucg acgaccugcu cgggagccgg cacuggucca aggacgccga caccagcccg     2340

accaugcgca agaaccggga cgacaaccgc caccacggcc uggacgccau cgucgcccug     2400

uacugcuccc ggagccucgu gcagaugauc aacaccaugu ccgagcaggg gaagcgcgcc     2460

guggagaucg aggcgaugau ccccaucccc ggcuacgcca gcgagccgaa ccuguccuuc     2520

gaggcccagc gggagcuguu ccgcaagaag auccucgagu ucauggaccu gcacgccuuc     2580

gucagcauga agacggacaa cgacgccaac ggcgcgcugc ucaaggacac cguguacagc     2640

auccuggggg ccgacaccca gggcgaggac cugguguucg ucgugaagaa gaagaucaag     2700

gacaucgggg ugaagaucgg cgacuacgag gaggucgccu ccgccauccg ggggcgcauc     2760

accgacaagc agcccaagug guaccccaug gagaugaagg acaagaucga gcagcuccag     2820

agcaagaacg aggccgcgcu gcagaaguac aaggaguccc uggugcaggc cgccgccgug     2880

cucgaggaga gcaaccggaa gcugaucgag uccggcaaga agcccaucca gcugagcgag     2940

aagaccaucu ccaagaaggc gcucgagcug gucggcgggu acuacuaccu gaucagcaac     3000

aacaagcgca cgaagaccuu cguggugaag gagccgucca acgaggucaa gggcuucgcc     3060

uucgacaccg ggagcaaccu cugccuggac uucuaccacg acgcccaggg caagcugugc     3120

ggggagauca uccggaagau ccaggccaug aaccccuccu acaagcccgc cuacaugaag     3180

cagggcuaca gccucuacgu gcgccuguac cagggcgacg ugugcgagcu gcgcgcgucc     3240

gaccucaccg aggccgagag caaccuggcc aagacgaccc acguccggcu gcccaacgcc     3300

aagccggggc gcaccuucgu gaucaucauc accuucacgg agaugggcuc cggguaccag     3360

aucuacuuca gcaaccucgc caaguccaag aagggccagg acaccagcuu cacccugacc     3420

acgaucaaga acuacgacgu gcggaagguc cagcugucca gcgcggggcu cgugcgcuac     3480

gugagccccc ugcuggucga caagaucgag aaggacgagg uggcccucug cggcgaguga     3540


<210>  1714
<211>  3567
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1714
augaaguaca uccugggccu cgacaucggg gugagcuccg ucggcaucgc cgugaucagc       60

gaggagaacc agaagaagac caucgagaac cuggccgugc gcaucguccc cgaggacccg      120

gacuuccacg ggaaguucua cuccggcaac accgcgagca agaaccugga gcggacggcc      180

aagcgcggga accggaagaa caaccagcgc uucaaggccc ggcgcgacaa gcucuaccag      240

ucccugaagg agaacaacau guuccccacc agcaaccugu ucgaccucuc cgccguggag      300

cuguacggcc ugcgggccaa ggcggugagc gagcaggucu cccuccagga gcugggccgc      360

auccugaucc uccugaacca gcggcgcggg uuccugagca accggaaguc cgugagcgag      420

gagaacucca ccgaguacaa ggagcgcauc gccggccucg agaaggcccu ggggaacaag      480

accaucggcc agaagcugua cagcgagcuc caggagaaca acaacguguu cgagguccug      540

cugcgggagc gcacguacca gcgguccagc uacaccgagg aguucgaccg caucugggac      600

gagcagaaga aguacuaccu cauccugacc ggguccagca acgaggacga caacaagggc      660

acccuguacg accucauccg gaaccgcauc aucuacuacc agcggccccu gaagucccag      720

aagggccuga ucagcgagug ccucuucgag aagcaccaca aggccguggc caagagcucc      780

cccuacuucg agcuguuccg caucuggcag aagaucaacg accugagcug gaagacgccg      840

aacggggagc uguucaagcc caccgacgag cagaagcaga agcucaagaa cgcgcugugg      900

aacggcgaca accucaaccc caagcacaag cugaccgugu ccaagaucaa ggagauccug      960

ggguacacca gccgggagaa gaucuaccuc aacuucacgg agcuggacgg cucccgcacc     1020

uacagcaucc ugcgcaacgc ccucaucgag gccggcaucg agaagcccga gcaguaccug     1080

uucuucaaca uggagaccaa cgacgagaag ggcgggcugu ucgagcucug gcacaucacc     1140

uacucccugc cgaucgagaa ggaggucauc aacgcccuga agaaccgguu cggguucgac     1200

aagaagcagg cggagaucau cgcccgccgg gugggcuaca gcuccgacua cgggagccuc     1260

uccacgcgcg ccauccggaa gcugcuggug caccucgaga agggccuggg cuacaccgac     1320

gccugcgaca acaucgggua cgaccacagc ggcuacaaga ccaagaucga ccugcagccc     1380

aagcugaagc agcucaagcg caacgagcug cggaaccccg ucguggagca gauccugaac     1440

caggugguca acgccgugaa ccucaucauc gagaaguacg ggaagaucga cgagauccgc     1500

guggagcugg cgcgggagcu gcgcaacaac gccaagaccc ggaagaacau cucccuccug     1560

aacagcaaga acaagaagaa caacgacauc auccgcaaga agcugcagga ggaguacggc     1620

uucaaguucg ucaacgggcg ggacgugacg cgcuacaucc ucugggagga gaacuccaag     1680

gagugccugu acugcaacaa gaccaucaac ggcagcgaca ugcugaccgg caacgccgac     1740

aucgagcaca uccuccccaa gucccggagc uucaacaacg ccaugaacaa cuacaucauc     1800

acccaccgca agugcaacuc cgacaagggg cagaagacgg ccuacgacuu caugagcagc     1860

aagggcgagg ugauccugca gcaguacauc gagaagguca acgaccugua caaggacggg     1920

aagggcucca ucagcaagac caaguucgag aaccuccugu gcaaggggga ggacaucccg     1980

uccgacuucg uggagcggau gaagaaggac agccaguaca ucgcgaagga ggccgucaag     2040

augcugaaga ccgugugccc caacguguac accacgaccg gccaggucac cgacuuccuc     2100

cgcgaggagu ggaagcugaa gaaccugcuc caggagcuga ccuucgagaa guacaaggcc     2160

aucggccagg ugaaggagaa gaucaucaag acgaacaacg ggcagaccaa gaccuucaag     2220

gugaucgccg acugguccaa gcgggacgac caccgccacc acgccaucga cgcgcugauc     2280

ugcgcccuca ccgaccagaa gaucaucuuc aagcugaaca accugaacaa gaucuaccag     2340

uacgagcgca acgcccucag ccagaaggag aucgaggaga ucgaggaguu ccuggagggc     2400

aaguucucca ucaaggaguu cagcgcccag caggggaaca ucaucgagug cuccaucccc     2460

aacauccgga aggacgcgca gaagcaccug gagaacaucc ucaucagcuu caagaaggag     2520

aacuccaagg uccugacgau gaaccgcaac cagcccaaga acugcaagga gcagaucacc     2580

ugggugccgc gggcccgccu gcacgaggac accaucaugg gccgggugaa gcgcaucccc     2640

gagaagaaga ugaagcucaa cgacaagguc agcuucgagc agaucaagau gaucgugaac     2700

cccgagauca aggagauccu gcuguaccgg cucgccaagu ucggcgacaa ccccaaggug     2760

gccuucgcca ccaaggcgcu gaagaaggag ccgaucuucu accgcgagaa cgagcugaag     2820

gaggucucca ucuacgagug ggugaacacc aagcgggugg cccucaacga gaacaucacg     2880

gacagccaga ucgagaagau caucgaccag gccuccaagc gcgccgucaa ggagcggcug     2940

aacgggggca agaucaagga gguguucaag aaccugaccg agaaccccau ccagcucaag     3000

aacgggauga uccugaagaa cgugaccguc accgacgaga gcaaggugga gaagauccgc     3060

aacggcuucg ccaucacggg gaagaaccac cacgcgcugg uguacaagga cgagaacggc     3120

aaguacaacg acaaggucgu guccuucugg gaggccgugg ccaucggccu ccggaacguc     3180

accgaguacg ggaaccccua ccccaucauc aaccgcaacc acaacgacga gcugggcgag     3240

uucuacuuca gcaugcagau caacgaccug uucguguucg accucaagca cagcgagaac     3300

ccgcaggagg agaacgagau caacuucuuc gacguggaga accggaagca gaucuccgac     3360

aagcuguucc gcguccagaa gaugagcaag aaguccaacg ggcaguucgu gagcgacuuc     3420

cggcaccacc uggagaccuc cgugauccgc aacgacauga uccucaagaa caucaccugg     3480

aucagcaucu ccaagaacag cgaccuggag cggcugacga agauccgcgu caaccaccuc     3540

ggcgagauca ucaagguggg ggaguga                                         3567


<210>  1715
<211>  3585
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1715
auggacuuca ccgccgacgu gagcgagcug aacgaguccu ucaugcagau ccacaccgag       60

agcaaguucg agaucgacac ggccaagguc caggagaucu cccagcuccu gcuggacaac      120

aagaucaccc gcauggaccg gcagaagcag gucgcgaacg ugcucgccaa gaacagcggc      180

gacaaggagg ccgacaagau caacaagaag aucgccaccg aguucugcaa ggccgugcug      240

gggaacaagg cgaaguucaa cgugauccug ggcgaggacg ccguggacuc cgacuggagc      300

uucaagaugg acgacgagaa cuccgacgag gagaucgaga uccucauggg gcagaccaac      360

ggccagcagc agaacguccu gcgcaucauc caccaguggu acagccagau cacgcugaac      420

gggaucgugc ccgacggcca gaugaucucc gagagcauga ucgacaagua cgagcuccac      480

cggaagcacc uccugcugcu gaagcagcag aucaagguga ccaaggacaa gaagaaggcc      540

cagcagcugc acgaggccua cgagcaguac guccacggca ccggggcgaa gaagaagcuc      600

acccaggacg acuucuacuc caaggugacg aagaaccugg agaccagcga cuucuccgac      660

gagaucaaga agcagaucaa cgccgagacc uucaugccga agcagcgcac cagcgccaac      720

ggcgugaucc cccaccagcu gcaccagcag gagcucgaca agaucaucga gaagcaggcc      780

aaguacuacc ccuuccuggc cgagcugaac cccgacacga agcggcagaa caucgcgaag      840

uacaagcucg acgagcugau cgccuucaag aucccguacu acgucgggcc ccugaucacc      900

accgaggacc agaagaagac cuccggcaag agcuucgccu ggauggugcg caaggagccc      960

gggaagauca cgcccuggaa cuucgaccag aagguggaca agauggccuc cgccgaccag     1020

uucauccggc gcaugaccac caaggacagc uaccucauca ccgaggacgu ccugccggcg     1080

gagucccuga ucuaccagaa guacaaggug cucaacgagc ugaacaugau ccgggugaac     1140

ggcaacaagc ugacgaagca cgacaagcag aacaucuacc agcaggucuu cgagaaguac     1200

aagaccguga acgugaagaa gguccagaac uacuuccuca ccgacggccg cuaccagcac     1260

gcccccaaga ucaccgggcu gagcgacgac aacaaguuca acagcaaccu guccacguac     1320

aucgacuuca agaagaucuu ccaggacgug guggacgacc cccagaagca gaaggacuuc     1380

gagcggauca ucgaguggag caccaucuuc gaggaccgcc acaucuacga ggucaagcuc     1440

aaggagaucu ccuggcugaa cgacgagcag cggaaggagc ugaagaagaa gcgccaccag     1500

ggcugggggc ggcucagcgc caagcugcug accggcaucu ucgacgagaa cggcuacucc     1560

aucauggacc agcucuggaa cacccagaag aacuucaugc agauccaggc cgaggagggg     1620

uucaacaaga agauccaggc cgagaacagc aagcaccugg agaagaacga cuucgaggac     1680

auccuggagg acgcguacac gagcccccag aacaagaagg ccauccgcca ggugaucaag     1740

gucguggagg acauccagaa gaccaugggc accgccccgc agaagaucag caucgaguuc     1800

acccgguccg ccgacaagaa cccccgccgg agcaucgagc gccagcggca gcucgaggag     1860

aucuacuaca acgacgccaa ggagaucgug uccgagggga ugcaggacaa ccugcagaac     1920

uucacggaca acaagcagac ccugaccgac aaguacuucc ucuacuucac ccagcugggc     1980

aucgacaugu acacggggaa gaagaucaac aucgacgaga ucggcaacua ccagaucgac     2040

cacauccugc cccaggcguu caucaaggac gacagcaucg acaacaaggu ccucgugucc     2100

agcgccauca acaacggcaa guccgacaac gugccccuga gcauguacgc cuccguccgc     2160

aucaagagcg aggugcagaa caaguggaag aacuucgccg agcacgggcu gaucucccag     2220

cgcaagcuga acaaccucau gaccgacccg gagagcaucg acaaguacaa ggcgcggggc     2280

uucaucaagc gccagcuggu ggagaccagc caggucaucc ggcugguggc cgagauccuc     2340

aacgagaagu accaggggga gaccgagauc aucgaggugc gcgccaagau gaacucccag     2400

cugcggaagg acuucgaccu gcucaagaac cgcaacguca acgacuacca ccacgcccug     2460

gacgccuacc ugaccacguu caucggcaac uaccucuacc ggcgcuaccc caagcugcgg     2520

agcuacuuca ccuacgggca cuucaagaag aucgacgacg cgaagcugaa gcaguucaac     2580

uuccuccacg acaucgagaa cgacaaggac cccaaguacg ugaacgacaa cggcgagcug     2640

cugugggacc gcgagaagga caucgccuac uucaagaaga ucuacaacuu caaguacaug     2700

cucguguccc acgaggucag cacccggcag ggcgccaugu ucaagcagac caucuucccc     2760

gccgccgcgg acgccaaguc caagcugauc ccgaucaaga acgacaagcc cacggccauc     2820

uacgggggcu acaccaacaa caacgacgcc uaccuggcca ucaucaagcu cgcgaagacc     2880

gggcaguuca aggugguggg cgucccccag cgcgccaucg ugaagcugaa gcaggccuac     2940

gacaaggaga ccggggacua cagcaacgug cuguacaagg uccucgagcc caaguucgug     3000

aagaagaagg ugaaccggaa gacgggcgag aucaccuacc aggucgagaa guucaagauc     3060

guggugccga agguccccua ccgccagcug gugaucgacg gcgaccagaa guucaugcug     3120

ggguccagca ccuacaagua caacgccaag cagcucgugc uguccgagaa cgcgaucaag     3180

auccuggaca agaaguccuu cgaccagucc ggcuacaaga gcuccgaccu cgacaccguc     3240

uacgacgaga uccugagcca gguggaccgg uacuucucca uguacgacaa gaacaaguuc     3300

cgcgaggggc ugcacaacgg caagaaguac uuccagcggc uccccaugau cgacaacgug     3360

gagaagggca agaucaagga gaacgggaag uucaagcugc ugaacgagau ccucaucggc     3420

cugcacgcca accccagcuu cgggaaccug aagucccucg gcaucagcac gccguucggg     3480

augaugcagu ucgccagcgg caucucccug agcgagaacg ccauccugug cuaccagucc     3540

cccaccggca ucuucgagcg ccgggucgcc cucaaggacc uguga                     3585


<210>  1716
<211>  3588
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1716
augcagaaga acaucaacac caagcagaac cacaucuaca ucaagcaggc ccagaagauc       60

aaggagaagc ugggcgacaa gcccuaccgc aucgggcucg accugggcgu ggggagcauc      120

ggcuucgcca ucgucuccau ggaggagaac gacgggaacg ugcugcuccc gaaggagauc      180

aucauggugg gcagccggau cuucaaggcg uccgccggcg ccgccgaccg caagcugagc      240

cgggggcagc gcaacaacca ccggcacacc cgcgagcgga ugcgcuaccu guggaagguc      300

cucgccgagc agaagcuggc gcugcccgug cccgccgacc ucgaccggaa ggagaacucc      360

agcgagggcg agacguccgc caagcgcuuc cugggggacg ugcugcagaa ggacaucuac      420

gagcuccggg ucaagagccu ggacgagcgc cugucccucc aggagcuggg cuacgugcug      480

uaccacaucg ccgggcaccg gggcagcucc gcgauccgca ccuucgagaa cgacagcgag      540

gaggcccaga aggagaacac cgagaacaag aagaucgccg gcaacaucaa gcggcucaug      600

gccaagaaga acuaccgcac cuacggggag uaccuguaca aggaguucuu cgagaacaag      660

gagaagcaca agcgggagaa gaucuccaac gccgcgaaca accacaaguu cagccccacg      720

cgcgaccugg ugaucaagga ggccgaggcc auccucaaga agcaggccgg caaggacggg      780

uuccacaagg agcugaccga ggaguacauc gagaagcuga ccaaggccau cggcuacgag      840

uccgagaagc ucaucccgga gagcggcuuc ugccccuacc ugaaggacga gaagcggcug      900

cccgcguccc acaagcucaa cgaggagcgc cggcuguggg agacccugaa caacgcccgc      960

uacagcgacc ccaucgucga caucgugacg ggggagauca ccggcuacua cgagaagcag     1020

uucaccaagg agcagaagca gaagcuguuc gacuaccucc ugaccggguc cgagcucacg     1080

ccggcccaga ccaagaagcu gcugggccuc aagaacacca acuucgagga caucauccug     1140

caggggcgcg acaagaaggc ccagaagauc aagggcuaca agcugaucaa gcucgagagc     1200

augcccuucu gggcccggcu gagcgaggcg cagcaggacu ccuuccugua cgacuggaac     1260

agcugccccg acgagaagcu ccugaccgag aagcugucca acgaguacca ccucacggag     1320

gaggagaucg acaacgccuu caacgagauc gugcugagcu ccagcuacgc gccccugggc     1380

aaguccgcca ugcucaucau ccuggagaag aucaagaacg accugagcua caccgaggcc     1440

gucgaggagg cccugaagga ggggaagcuc accaaggaga agcaggccau caaggaccgc     1500

cugccguacu acggcgccgu gcugcaggag uccacccaga agaucaucgc caagggguuc     1560

agcccccagu ucaaggacaa gggcuacaag acgccccaca ccaacaagua cgagcucgag     1620

uacgggcgga ucgcgaaccc cgugguccac cagacccuga acgagcugcg caagcucgug     1680

aacgagauca ucgacauccu gggcaagaag ccgugcgaga ucggccugga gaccgcccgg     1740

gagcucaaga aguccgccga ggaccgcagc aagcuguccc gggagcagaa cgacaacgag     1800

agcaaccgca accggaucua cgagaucuac auccgccccc agcagcaggu caucaucacg     1860

cggcgcgaga acccccggaa cuacauccug aaguucgagc uccuggagga gcagaagucc     1920

cagugccccu ucugcggggg ccagaucagc ccgaacgaca ucaucaacaa ccaggccgac     1980

aucgagcacc uguuccccau cgccgagucc gaggacaacg ggcgcaacaa ccucgugauc     2040

agccacuccg cgugcaacgc cgacaaggcc aagcggagcc ccugggccgc cuucgcgagc     2100

gccgccaagg acuccaagua cgacuacaac cgcauccuga gcaacgugaa ggagaacauc     2160

ccccacaagg ccuggcgguu caaccagggc gccuucgaga aguucaucga gaacaagccg     2220

auggcggccc gcuucaagac cgacaacucc uacaucagca agguggccca caaguaccug     2280

gccugccucu ucgagaagcc caacaucauc ugcgucaagg ggucccugac cgcgcagcug     2340

cggauggccu ggggccucca gggccugaug auccccuucg ccaagcagcu gaucaccgag     2400

aaggagagcg aguccuucaa caaggacgug aacagcaaca agaagauccg ccucgacaac     2460

cgccaccacg cccuggacgc caucgugauc gcguacgccu cccgggggua cggcaaccug     2520

cucaacaaga uggccgggaa ggacuacaag aucaacuaca gcgagcgcaa cuggcugucc     2580

aagauccugc ugcccccgaa caacaucguc ugggagaaca ucgacgccga ccucgagagc     2640

uucgagucca gcgugaagac ggcccugaag aacgcguuca ucuccgucaa gcacgaccac     2700

agcgacaacg gcgagcucgu gaagggcacc auguacaaga ucuucuacuc cgagcggggg     2760

uacacccuga ccacguacaa gaagcugagc gcccucaagc ugaccgaccc ccagaagaag     2820

aagaccccca aggacuuccu ggagaccgcc cuccugaagu ucaagggccg cgagagcgag     2880

augaagaacg agaagaucaa guccgccauc gagaacaaca agcggcuguu cgacgugauc     2940

caggacaacc ucgagaaggc caagaagcug cuggaggagg agaacgagaa guccaaggcg     3000

gaggggaaga aggagaagaa caucaacgac gccagcaucu accagaaggc caucagccuc     3060

uccggcgaca aguacgucca gcugagcaag aaggagccgg ggaaguucuu cgccaucucc     3120

aagcccacgc ccaccaccac cggcuacggc uacgacaccg gggacagccu gugcguggac     3180

cucuacuacg acaacaaggg caagcugugc ggggagauca uccgcaagau cgacgcgcag     3240

cagaagaacc cccugaagua caaggagcag ggcuucaccc ucuucgagcg gaucuacggg     3300

ggcgacaucc uggaggugga cuucgacauc cacuccgaca agaacagcuu ccgcaacaac     3360

acgggcuccg ccccggagaa ccgggucuuc aucaaggugg ggaccuucac cgagaucacg     3420

aacaacaaca uccagaucug guucggcaac aucaucaaga gcaccggggg ccaggacgac     3480

uccuucacca ucaacagcau gcagcaguac aacccccgca agcugauccu cuccagcugc     3540

ggguucauca aguaccgguc ccccauccug aagaacaagg agggcuga                  3588


<210>  1717
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1717
augagcaagc acgugcuggg ccucgaccug ggggucggcu ccaucgggug gugccugauc       60

gcccucgacg agcaggacca gcccgccgag auccugggca uggggagccg caucgugccg      120

cugaccaccg acgaccucaa cgaguucucc cagggcaagg cgaucagcaa gaaccagaag      180

cggacgaccc agcgcaccau ccggaagggc cuggaccgcu accagcugcg gcgcaaggug      240

cucuccgaga agcuggccgg gcacggcaug cugcccgaca aggaccucau gcagcugccc      300

guccuggacc ucugggagcu gcgggcccgc gccgccagcc ccggggagaa gcugucccuc      360

ccggagaucg gccgggugcu guaccacauc aaccagaagc gcggguacaa gcacgccaag      420

agcgacgagg cggaggaggu gaccaagcgg ggcaaggagc agggcuacgu cgccacggug      480

aaggggcgcu acgccgagcu gcagcgggag ggcgcgaccg uggggcagca cuucgccugc      540

aagcucaagg agcaccgcca ggaguccccc agcggcggcu ucuucuacaa cuaccggauc      600

aaggaccagg ucuacccccg cgaggccuac aucgaggagu ucgaccggau cauggaggug      660

caguccgugu ucuaccccga gguccugacc ccggccuuca uccaggagcu gcgcgacaag      720

gugaucuucu uccagcggcc ccucaagagc ugcaaggggc ugguguccuu cugcgaguuc      780

gagaagcagg agcgcgucgu gcagcugaag cgggugaacc gcgagggcaa ggaggagcuc      840

caggagcgca agaucaccau cgggcccaag gucgccccca agagcucccc gaucgcgcag      900

cugugcaagc ugugggagag cauccacaac auccggaucu acuaccccga cggcaaggag      960

cgccccauca cgcucgagga gaagcggaag cugcuggccu accuccagac ccacgacaag     1020

cugaccuuca ccgcccugaa gaaggagcuc aagaucuccg acaaggagcg ccuguggugc     1080

gacaagcugc ucaagggcgg gaucaagggc gacacgaccc gggugcugcu gcgcaaggcc     1140

cucgccgggu acaagcggua cgagccccug cugcgcaugg accucgagcu gcgggacuuc     1200

caggagaucg ugagcggcaa gcgccugauc cggccgaucg ucgacgaguc cuaccucagc     1260

cagccccugu accgccugug gcacauccug uacagcaucg aggagcggga ggcgaugcgc     1320

cgggcccuca ucgaccagcu gggguuccug gaggaggacc ucgacggcgg gcugcuggac     1380

aagcucuacc agaucgacuu cgccaagcag ggcuacucca acaagagcgc caaguucauc     1440

ugcaagcugc ugccccaccu ccagggcggg cacggcuacu cccaggccug cgagcuggcg     1500

gggaagcgcc acagcgccgg cucccugacc aaggaggagc ucgaggagcg gccccugcug     1560

gagcacaucc cgcuccugca gugcaacgag cugcgccagc ccaucgugga gaagauccuc     1620

aaccagauga ucaaccuggu gaacgcccug cggcagaagu acgggagcau cgacgagguc     1680

cgcguggagc ucgcccgcga gcugaagaug ucccgggagg agcgcgagga gaccagcaag     1740

cggauccgcu cccgggagaa ggagaacaag gagaucgccg agcgcaucaa ggaguacgag     1800

cuguacccca ccaagagccg gauccagaag uaccgccucu ggcaggaggc gggcgagcag     1860

ugccuguacu gcggccgguc caucgacguc gugcaguucc ugcgcgggga cggcguggag     1920

aucgagcaca ucauccccaa gagcauccuc uacgacgacu ccagcgccaa caagacgugc     1980

gccugcuccc ggugcaacaa ggacaagggg aaccgcaccg ccuacgagua cauccuggcg     2040

gagggccggg aggccgagua ccuggaccgc cucgagaccc uguuccacga cggcaagcug     2100

agcuacucca agcggcgccg gcuccagugg aagaaggccg acaucccggu cgacuucauc     2160

gagcgccagc ugcggcugac gcaguacauc gcccgccagg cccaggagau ccuccggcag     2220

gggauccgcc gcgugggcgc gagcgagggg uccgugaccg ccaagcugcg gcaccugugg     2280

ggcuacgacg acauccuccg cgagcugaac uucgagcggu accagagcau gggggagacc     2340

gagaucguca gccaggagga gaaggacggc uccaucacca agagcgagcg caucaaggac     2400

ugguccaagc ggaaggacca ccgccaccac gccaucgacg cccugguggu ggccugcacg     2460

cggcagggcu acauccagcg ccucaacagc cugaacuccg aggaggacag ccugcagaug     2520

caggaggacu ccaagcagau gcgggagaag augggcgggc agaagcgccc caccgcggac     2580

aagcacagca uccucgagaa cuggcugauc cugcagcccc acuucuccca ccaggagguc     2640

aagagcaagg uggccgagau ccucgugucc uuccggcccg ggaagcgcac cgucagcacc     2700

ggccggaaca cguaccugcg cuccgggaag aaggugaccc agagcggcgu gcugaucccg     2760

cggggccccc ucuccgagga gagccuguac gggcagaucc ugcgcaacgg cgagcccacc     2820

uacgucaaga agguggcccu cgagucccug agcgaggccc agaucgagca gaucguggac     2880

ccgaagcugc ggcgcgagau ggucgcccgg acccagccca gcgagggggc gaagcucaac     2940

cccaagcugc ugcucgccac gcccuucuac uccgacgccg ccggcaccca gcgcgugcgc     3000

agcgugcggg ucgcgaucga gaacgggaag aacuccaucg ugccgcugcg cuacaacgag     3060

agcggcaagg ccaucaccuu cgugaacccc ggcaacaacc accacgucgg gaucuaccgg     3120

accuccgagg gcaagcuggu ggagagcugc gugacguucu ggcaggccgu cgaccgcgug     3180

cgguacggga uccccaccau cauccgcaag ccccgggagg ugcucgacca gcugcugcuc     3240

cgcgacgaca ucccggaggc ccugcuggcc caccuccccg cguccgacug ggaguucguc     3300

gaguaccugc aggccaacga gauguuccug aucggccuca ccgacgagga gauccagacc     3360

gcccuggccg agagcgaccu gcucacccug uccaaccacc uguaccgggu gcagaagaug     3420

agcuccgagu acuacguguu ccgcuaccac cucgagacgg aggucgccga caacaagaac     3480

aacaccggga ucauccccaa guucuaccgg cugcgcggcc ccaacgacac cuaccugaag     3540

gcgaacccgc ggaaggugaa ggugaaccuc cugggcgaga ucagccugcc cuga           3594


<210>  1718
<211>  3615
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1718
auggagcgca uccugggcau cgacaccggg accaacagcc ucggcugggc caucguggac       60

aaggacgaga accaguacca ccuggucgcc aaggggacga acaucuucuc cgagggcgug      120

aagaucgaga aggggaucga gagcuccaag gcggccgagc ggaccgagca ccgcagcgug      180

cggaagcacu acuggcgccg gaagauccgc aagauccggc ugcucaccau ccuguccgac      240

aacgaccugu gcccccgccu caagcacgag cagcugcggc aguggcgccu gaagaagguc      300

uacccgaccg acgacacguu cauggagugg cagcggaccg aggacaaggc ccaggugaac      360

cccuacaagu uccgcuacau cugccucacc cagaagcugg accugagcga caucacccag      420

cgguucaucc ucggccgcgc ccuguaccac cugaaccagc ggcgcggcuu ccucuccaac      480

cggaaggagg ccacgaagga gagcgagggg accgugaagg agggcaucuc cagccugucc      540

aaggacauga aggacagcgg gugcgaguac cugggcgagu acuucuacca gcucuaccag      600

caggggaaga agauccgcaa ccacuacacc gcgcggaagg agcacuaccu gaaggaguuc      660

aaggccaucu gcuccaagca ggagcuggac gacgcccuca ucgagaagcu ggagaaggcc      720

aucuucgacc agcgcccccu caagagccag aagggccagg ucgggaccug cacguucgag      780

aagggcaagu cccggugccc cagcucccac ccgcuguacg aggacuuccg cauguacagc      840

uucaucaaca acaucaaggu ccagguggac acccuguacg acaagggcga gcgcucccug      900

accgacgagg agaagcagcu caucaucccc cuguucaagc gcaagagcaa gcggaccuuc      960

cccuucgagg acaucgcgaa gaagaucgcc gggaagaaca acuacugcua caucaagagc     1020

aacgaggaga aggccuacaa guucaacuac cagauggaca cccagguguc cggcagcccc     1080

gugaacgccc agcuggagga gaucuucggc gaggacuggc ucuccagcgu gugcgagguc     1140

uacacgcugg ccaacaacaa gucccgguuc cagaucauga acgacgugug gcacgugcug     1200

uucuucuacg acgacgagga caagcucaag gaguucgcga aggaccgccu gcagcuggac     1260

gacgagcucg ccgagaaguu cagcaagauc uccaucccga acgacuacgc cgcccugagc     1320

cugaaggcca uccggaagau caugcccuac cuccgcgacu acgggcugau cuacuccgag     1380

gcggucuucc uggccaaccu cguggacgug cugccccagc acgucugggg caucaaggag     1440

augcgggagg ccgccaucga gaacgugauc gacgugaugc acagcuacga caagaacaac     1500

aucgacggga ucacccugga gggcugcguc aagaccuucc ucaaggagcg cuaccaggug     1560

uccgacgaca accugaagaa gcuguaccac cccagcaugc ucgacccgua cccccggcag     1620

cgccccaacg acgagggggu guuccagcug ggcagccccc ggaucagcuc cgucaagaac     1680

ccgauggcca ugcacagccu guuccgccug cggaaggugg ucaaccucau gcugaagaag     1740

ggcuacauca cgcccgacac caaggugcac aucgaguucu cccgcgaccu gaacgacgcg     1800

aacaagcggu gggccaucca gaccuggcag cgcgagaacg agaaggagcg ggagcgcugc     1860

cggaaggaga ucgagaagua caucgccagc cccuccgaca ccgacauccu caaguaccag     1920

cugugggagg agcagaacca caagugccug uacacggggg acgagauccg caucaccgac     1980

uuccucggcg acaaccccaa guacgacauc gagcacacca ucccgcgcag cgccgggggc     2040

gacagcacca agaugaaccu gacgcugugc uccagccggu ucaaccgcga cgagaagaag     2100

accaagcucc ccgccgagcu ggugaaccac gacgagaucc uggagcggau cuccaacugg     2160

aaggagaagu acgaggacau cgacaagcag auccgcaaga ucagcaccaa gggggcgacc     2220

uccaaggagg acaaggacag caagauccgg cgccggcacu accucauccu gcagcgcgac     2280

uacuggcggg gcaaguacca gcgcuucacg augaccgagg uccccgaggg cuucucccgg     2340

cgccagggga ccgacaucag cgugaucucc cgguacgccc gcauguaccu caagagcgug     2400

uucaagcagg ucuacaucgu gaagggcauc gccaccuccg acuuccggaa gauguggggg     2460

auccaggacg aguacgccaa gaaggagcgc gugaaccacg uccaccacug caucgacgcg     2520

aucacgaucg ccugcaucgg caaggccgag uacgacaagc uggccgacua ccggcaccag     2580

uacgacgacu accuguucca ggggaagacc aagcccaccu ucgacaagcc guggcccagc     2640

uucgugaucg acaucaagca cauccaggac gagcugcuca ucgcccacua cgcgaaggac     2700

aaccuggaga aguucaccaa gaagaaccug cgcggcaagg acggccggau cauccgcgac     2760

aaguucggga acaagcucca gaugaagggc gacaccuccc ggaacagccu gcacaacgac     2820

aaguucuacg gggccaucaa ccacgagggc gagaucaagu acguguaccg cacgaacauc     2880

gaggcccugg acgccaaguc caucggcaac aucgucgacc ccaccgugcg cagcaucauc     2940

gaguccgcca ucgcgaucca cgggagccuc aaggccgcca ucaacaaggg caucuggaug     3000

aacgaggaga agcacauccc caucaagaag gugcgggucu uccgcaacga cgugaagaag     3060

ccgaucgagc ugaaggacca gcgggaccug agcgacaagg acuacaagca gcacuacuac     3120

gugaccaacg acgggaacua caugaugcuc aucuucucca agcugaacaa caagggccgc     3180

gagauccggc agcacgucgu gaucaacagc cuggaggcca ccgaggugau caagucccac     3240

aagcgcagcc aggucgggaa gguggugugg gaguccuacu acagcaagga gggcuucgac     3300

cucaucuaca agcugaaguc cggcacgaug auccugcucu acgagaacaa ccgggacgag     3360

gucugggacc uggacauccg caaccugcag aagcggcucu acaucaucac caagaugaag     3420

uacgacgggc gcaucuacau gaugcaccac caggaggccc gggagaagaa ggaccuggag     3480

gagaaguccg gcuccuucaa gaucaacgag gacuaccgcc cccugaucgu gaacagcuac     3540

aaccaguuca acgcgcucgu gcaggaccug gacuucgaga ucaacgaccu gggggagauc     3600

cggcgccuca ucuga                                                      3615


<210>  1719
<211>  3630
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1719
augaagaagg acuacauccu gggccucgac aucgggauca gcuccguggg cugggggcug       60

cuggagcucg acgacaacaa caaccccuac aagaucaucg acgucggcag ccgcaucuuc      120

accccggggg agguggagaa gaccggcgac ucccgggcca aggagcgccg ggagaagcgc      180

ggcgcccggc gcaucgcgcg gcgccgggag uuccgccugg accgggugaa gaaccugcuc      240

uacgagaacg gguaccugaa gggcaacauc aacagcaaca ucgucuccga gaagaacgag      300

gagcugagca ucaucuucaa caacaugauc aacaacuacu acaacgggaa gaacacgaac      360

cccuacaucc ucaaggugga ggcccuggac cgcaagcuga ccgacgagga gcucuccauc      420

auccuggugc acuacgccaa gaagcggggc uacaagagca accgcgagga ggccuccgac      480

aaggagagcg ggaagguccu gaccgccauc aaggagaacg agaagaucau gaaggacaac      540

aacuaccgga ccaucuccga gauguacauc aaggacgaca aguucaagaa caagaucaag      600

aacagcccca acgacuacaa gaucuccgug acgaacgaga uguaccucga ggagaucaac      660

aaggugcugg acagccagau caaguucggc cugaucaccg acgacuucaa gaaccaguac      720

cucgagaucu acaacucccg ccggagcuac gcgaagggcc ccgggggcaa cuccccguac      780

gggggcgacc ugaucgagcg caugaccggc aagugcaacu ucgacgggaa cccccgggcc      840

cccaagcgcg ccuucagcuc cgagaucuac gucgcccuga ccaagcucgu gaaccugaag      900

uacaagguga acgacggcga guacaucaag cugagcgaga aggagaucaa gaacaucauc      960

gagaaggcga aggagaagaa guccaucagc uacaaggacc ucacgaagau gaucgggaac     1020

aacaucgacu ucaaggaccu gacccugagc aagaaggagu acaucaagcu caucgaggag     1080

cugaagaaga agcugaacau caagaaggac accaagauca acaucaacga gcucuccagc     1140

gaggacaagg agcuguacaa caagcugugc aacgacaagc uccuguccaa gacccugauc     1200

gagcucaagg gcuaccacau ccugaaggac agcgucauca aggccuacuc caaggacauc     1260

ugggacgagg ugaaggacaa cauguaccug cucgacgacc uggcccugua cugcacgaac     1320

uacaagguga acgaggacau ccucaacaag aucaaggaga gcgagaacau cgacaacauc     1380

uucaacgaca uccacuucgu cgagcugcug cccaacuuca aggaccaccu caugcugucc     1440

accgagcuga uccggaagcu caucccgauc auguacgggg gcaacaccua cgacaaggcc     1500

augaacaucc ugggcuacaa ccacagcgac aucaacgggg acaaggagaa gaaggaccug     1560

cuggugccca ucugcgugga cgacaacauc accaaccagc gcgucauccg cucccucacg     1620

cagacccgga aggugaucaa cagcaucauc aagaaguacg gcaugcccaa gaucaucaac     1680

aucgagaccg cccgcgagcu ggcgaagucc cggcaggagc gcaacgagau cgccaagaac     1740

aacguggaga agcaggccga gaacgagaag aucaagaacc agcuggucga gcucgggcug     1800

uucgugagca ccgacaagau cuccagcacg gaccugcuca aguacaagcu guggaaggag     1860

cagaacgagu ucugcggcua cuccaugaag aagaucagca ucgaggaccu guucgggaac     1920

aacaucgugc agaucgacca cauccucccc uacucccgga ccuacaacga caacuaccug     1980

aacaagaccc uggucuacac caaggagaac caggagaagg gcaaccgcac gccguacgag     2040

ugguucggca agaccgacaa gugggacgag uacgagaccu acaucaacaa ccucaccauc     2100

agccagaaga agaaggacaa cuaccugcug aagaagcucg acuucgacac ggagcgggag     2160

augcgcagcc agaaccugaa cgacaccaag uacaucucca aggagcugag cucccucauc     2220

aaggccuacc ugaacgugga gaaggucaac auguaccccg gggccaucac cgcgaagcug     2280

cgggcccgcu ggggcuucaa ccggcucacc cacagcuacc uguccaagaa cuacauccug     2340

cccaagaaca ucaaggagga caucaacaag gaccgcgaca accaccucca ccacgccaug     2400

gacgcccugg ugaucgccag caucacgccc ucccugcagc agaagaucac ccucuacgag     2460

aaguucagcc gguacaucga cgggcugacc gagcugcaga ucaagaacau cuccuacauc     2520

aagaucgagg acagcgaggg ccaguacuac aacgacgaca ccgacgaggu ggagacgauc     2580

gacauccgcg aguacaucaa gcagcagcuc gcggagaaca acaucuacua caaccggcac     2640

aacaucucca agcuggaguu cccgcugccc uacgagaacu ucgacaagga ggccaagcuc     2700

cgcgucuacg agcaggaccu ggacacccug aaguggaacc ucaagagcua caacaccuac     2760

acccagaagg acaucgacaa cgugcacacc cugauccccu ccaucgccaa gacgaaggug     2820

agcgggaaga ugcacgagga gaccuacuac ggcaucaagg agaucggcaa ggacgaggac     2880

aagaagaucu acaagacccu gcggaccccc aucgagaagg ucaagcgcaa ggaccucgac     2940

aacaucccgg accggcucgg gggcuccaag gacguguacg agagccuggu ggagugguuc     3000

ggggacuacg aggucggcga ggaggugcug aaggccaacg gcaacaagua ccccgugucc     3060

aagagcgaca aggagcacaa ggagaucaag aagcugaagc uguacagcga guacaagaac     3120

acggggcaca ugaucaacaa guccaacguc gagaagggcg ggaucuaccg caucgacgug     3180

uucaagagca aggacaagga ggacgacaag cucuacuucg ccgcguacga cauccuggag     3240

aucaagaaga ucaacacccu gaaggagaag aaccgcgugg acgacaacuu caacaucaag     3300

cucgacuacg gccaggggaa gaacuccaag aucaugagcu acaccgacau caucaacaac     3360

uacgaccugu acaucucccu gaacaagaac gaccucauca agaucaccac gaacgacggc     3420

aaggagagcg ucgccuacgu ggugggcugc uccagcggga ugcuggaggu caaguccaag     3480

cugggcgacg gguacgacau caucaagacc gaccucaacg agaacaacau cuucagcaag     3540

cagcgggccc aguaccagau caccgugucc accaucaagg agaucaagaa gcugagcauc     3600

uccgugcugg gcgagaucag cgggcucuga                                      3630


<210>  1720
<211>  3633
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1720
augcgcacca uccugggccu cgaccugggg accaacagca ucggcugggc cgugaucaac       60

uccgucauca aggagcagac ggagcggauc uggaucgaga uggccgggag ccgcaucauc      120

cccauggacg cggccauccu gggcgacuuc gacaagggga acuccaucag ccagaccgcc      180

gagcggaccc gcuuccgggg cgugcgccgg cuccgcgagc ggcagcugcu gcgccgggag      240

cgccuccaca aggugcugaa gauccugggc uuccucccgg agcacuaccu gaaggggauc      300

gacuucgaga agcacaccgg caaguuccug uccgggagcg agcccaagcu ccccuggguc      360

aaggaccggu ggggcaagua cuccuuccug uuccagacgg ccuucaacga gaugcuggcc      420

gacuucgcga agcaccagcc cagccucgug uucgacggga agaagauccc guacgacugg      480

accguguacu accugcgcaa gaaggcccug accgagauga ucuccaagga ggagcucgcc      540

uggauccugc ugaacuucaa ccagaagcgg ggcuacuacc agcuccgcgg cgaggaggag      600

gaggacaacg ccggcaagag cgucgaguuc uacgcgcuga aggugcuguc cguggaggcc      660

accgacgaga agaaggggcg ggacaucugg uacaacgucc accucgagaa cgggugggug      720

uaccgccgga gcuccaacgu gccccuggac ugggagggcc gcgucaagga guucaucgug      780

acgaccgacc ugaaccccga cggcaccccc aagcucgaca aguacgggga ggugaagcgg      840

agcuuccgcg ccccgaagga ggacgacugg augcugauca agaagcgcac cgaggccgac      900

aucacgggca cccacaagac caucgggucc uacaucuacg acgcccugcu ccaggagccc      960

cagcagaaga ucaucggcaa gcuggucaag accguggagc ggaaguucua caaggaggag     1020

cugaacuuca uccuccagaa gcagugcgcg uuccacgccg agcugcagga ccgcgaccug     1080

uacaugcggu gcaucgaggc ccucuacccc gggaacgagg ugcagcgccg gaacaucgcc     1140

aaccgcgacu ucaucuaccu gcugaucgac gacguccucu ucuaccagcg gccccugaag     1200

acgaagaagu cccugaucgc caacugcccg uacgaggaga acgaguacgu gaaccgcaag     1260

accggcgaga ucaagaccgc gccccucaag ugcaucgcca agagccaccc ccuguuccag     1320

gaguuccggc ugugguacuu ccucagcaac auccgcgugu accagaagga gcgggagguc     1380

aacggcaccc ugcaccugga cguggacgug acgaccgagu uccucaagac cgaggacgac     1440

uacgucgccc uguucgacug gcugaacucc cgcaaggaga ucgaccagaa ggccuuccug     1500

cgguaccccg ccuucgggcu caagaaggag aucaacaacu accgcuggaa cuacguggag     1560

gacaaguucu acccgugcaa cgagacccgg cacgcgaucc ugagccgccu ggagaaggcc     1620

ggcaucggga ucgacuuccu cagcgacgag aaggagacgg cccuguggca cauccuguac     1680

uccgucagcg acaagcagga gaucgagaag gcccucgcca ccuucgcggu gaagaacggc     1740

cugaacgagu ccuucgugga ggucuucaag aaguucccgc ccuucaagag cgaguacggg     1800

gccuacuccg ccaaggccau caagaagcug cucccccuga ugcggugcgg caaguacugg     1860

gacaugagcc ugaucgacgg caccaccaag gagcgcaucg agcggaucau cgcgggggag     1920

ugcgacgagu ccaucggcgc caaggugcgc gagaaggcca ugaaccucag cgacaucucc     1980

ugcuucaagc gccugccccu guggcucgcc ugcuacgugg ucuacaaccg gcacagcgag     2040

gacaaggaga ucacgaagug ggagaccccc gccgacaucg acgcguaccu ggccgccuuc     2100

aagcagcacu cccugcgcaa ccccaucgug gagcagguca ucaccgagac ccuccgggug     2160

gugcgcgaca ucuggaagca ggucgggaag aucgacgaga uccggaucga gcugggccgc     2220

gagaugaaga accccgccga caagcggaag cgcaugacga cccagauccu ggagaacgag     2280

aacgccaacc uccggaucaa ggcgcugcug gccgaguucg ugaacccgga guacggggug     2340

gagaacgugc gccccuacag ccccucccag caggagaucc uccggaucua cgaggacgcc     2400

guccugaagg gcgaggagca gauccccgag gacaucgacg ugauccugaa gaaguucaac     2460

accagcaagc ucccgaccaa guccgaguuc cugcgcuaca agcuguggcu cgagcagaag     2520

uaccggagcc ccuacacggg cgagcugauc ccccuggcca agcuguucac cgccgcguac     2580

gagaucgagc acgugauccc gcagucccgc uacuucgacg acagcuucuc caacaagguc     2640

aucugcgaga gcgccgugaa caagcucaag gacaaccagc ugggguacga guucaucaag     2700

aaccaccacg gccagaaggu ggaggucggg uucggcaaga ccguggagau cuucagcgug     2760

gacuccuacg agcgguucgu caaggagcag uacgccaaga gcggcaucaa gaugaagaag     2820

cuccugaugg acgacauccc cgagcaguuc aucgagcgcc agcugaacga cucccgguac     2880

aucagcaagg uggugaaggg gcuccugucc aacaucgucc gcgagaagaa cgacagcggc     2940

gaguacgagc ccgaggccgu guccaagaac auccuggugu gcaccgggag cgucaccgac     3000

cggcucaaga aggacugggg caugaacgac guguggaacu ccaucgugag cccccgcuuc     3060

gagcggcuga acgcgcugac ggagacccag ugcuucgggc acugggagaa caaggacggc     3120

aagaaggucu uccagaccgc cgugccgcuc gaguaccaga agggcuucuc caagaagcgc     3180

aucgaccacc gccaccacgc cauggacgcc aucgugaucg ccugcgcgac ccggaaccac     3240

gucaacuacc ugaacaacga gagcgccucc cgcaacgcca agaucagccg guacgaccug     3300

cagcgccucc ugugcgacaa gucccgcggg gacggcaacg ggaacuaccg guggaucauc     3360

aagaagcccu ggacgaccuu cacccaggac gcccgggagg cccugagcgg caucaucgug     3420

ucccucaagc agaaccugcg caucaucaac aagaccacga acaucuacca gcacuucgac     3480

gcgaacggga cccgggugua cgagaagcag aagaucggcg acagcugggc cauccgcaag     3540

cccaugcaca aggacaccgu cuucggcgcc gugaaccugc ggaaggcccu caucccccag     3600

cacuacaacc ugacccuguu caucagcucc uga                                  3633


<210>  1721
<211>  3642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1721
auggagaagc gccugggcau cgacgugggg accaacagcc ucggcugggc caucgucgag       60

guggacgacg aggggugcac ccugcuggac cggggcgugg acaucuucca ggaggggguc      120

gcccgcgaga agaaccagga gaagcccgcg gugcaggacc ggacggacgc ccgcgcccuc      180

cggcgccacu acuuccggcg ccggcugcgc aagaucgccc ugcuccgggu gcugguccgc      240

cacgaccugu gcccggcccu cuccgaggag cagcugaacg cguggcaggg caagggcauc      300

uacccccugg acgacgccuu cauccggugg cagcgcaccg acgacaacgc cgaccggaac      360

cccuaccgcg accgguuccg cugccucacc gaggagcugg accuggccug ccgggaggag      420

cgcuacgugc ucgggcgggc gcuguaccac cugugccagc gccgcggcuu ccucagcaac      480

cggaaggacg ccgggaacga ggccgaggag ggcaagguga agcaggagau caagacccug      540

uccgccgaca uggccgcggc cgggugcgag uaccugggcg aguacuucua ccgccucuac      600

cagcggaagg agaagauccg caagcacuac acggcccgga acgagcacua ccuggccgag      660

uuccgcgcca ucugccggaa gcagcgccug gacgaggagc uccggcaggc gcuggagaag      720

gccaucuucu accagcgccc ccucaagagc cagaagggcc uggucgggaa gugcaccuuc      780

gagaaggaca agucccggug cccggugagc cacccccgcu ucgaggaguu ccggaugcug      840

caguucauca acaacgugcg cgucaccggc cccggggacg ccgagccccg gccgcugucc      900

cccgaggagg uggagaucgu gcgcccccuc uuccugcgga aguccaagcc ccacuucgac      960

uucgaggaca ucgccaagaa gaucgccggc aagggcaacu acgcgugccg cggggagcug     1020

cuccaggccc cguaccgguu caacugcgcc ggcaagagca ccgucacggg gagccccgug     1080

uccgcccugu ucgccagcgu guucggcgac gacuggcagc gcgagaucug cucccuguac     1140

acccucgggg cgggcaagaa cgaggagcag auccugaacg acgucuggca cgugcuguuc     1200

gccuucgagg acgaggagcg ccuccaggcc uggcugcagg agaagcugca gcucagcgcc     1260

gacgacgcga agacccuggc cgccuuccgg cugccccagg gcuacgccuc ccucagccug     1320

aacgugauca acaagauccu gcccuaccuc cgccgggggu accgcuacga cgaggccguc     1380

uucuucgcga accugggcaa ggugcugccg gccgaggugu acgccgaccc cgcccggcgc     1440

cgggaggucg agcaggggau cuccgcccag auggacgacc ugggcgacgg ggagaagacc     1500

cgcaaggagg agcggauccg cgacuaccuc cggcgcuacu ggggcgugga ggagaagcgg     1560

cuggagcgcc uguaccaccc cagcaugauc gaggcguacc ccuccgccag cccgaacggc     1620

caggggcucc ugcagcuggg cagcccccgg auccccgccg uccgcaaccc cauggccaug     1680

cgggcccucu uccgccugcg ggugcugcuc aacgagcugc ugcgcgaggg gaagaucgac     1740

cggggcacga agaucaacau cgagcucgcg cgcgagcuga acgacgccaa caagcgcaag     1800

gccaucgagc gguaccagcg cgagcgggag gccgagaacc gcaaguacgc ggaggagauc     1860

cggcagcagu acguggaggc caccgggcac uccaucgagc cgagcgagga ggacauccug     1920

aaguaccgcc ucugggucga gcagggccac ugcucccugu acaccggcaa gaccaucggg     1980

aucagcgagu uccugggcga cgggcggcgc uacgacaucg agcacacggu gccccggucc     2040

cgcggcggcg aggacgccca guacaacaag acccucugcg aggaggaguu caaccgggac     2100

aucaagcgcg acaagcugcc cagcgagcug gccaaccaca ccgccauccu cgcgugcgug     2160

gaguccugcg ggugguucga gcggaucgag ggccuggagc accgcgucug gggcgccgac     2220

cggcgcgcca agagcgccac cacgaagucc gccaaggacu ucgcgaucca ggaccggcac     2280

uacuggcgca ugcaccugga cuacuggcgg cgcaagugcg aggccuucac caugaccgag     2340

gugcccgagg gguucagcaa ccgccagggg guggacgucg gcaucaucgg ccgguacgcc     2400

cgccucuacc ugcagaccgu guucgaccgg guguacacgg ucaagggggc caccaccgcc     2460

gcguuccgcc ggaugugggg ccugcaggag gaguacgccg ccaaggagcg cgugaaccac     2520

gugcaccacu gcaucgacgc caucaccauc gcgugcaucg ggucccggga guacgcccuc     2580

ugggcccagu acguccgcca ggacgagcug uaccgguggg gcgaggccga caagccgcgc     2640

ugcgggaagc ccuggcccac guucaccgag gacgugcggg ccaccgcgga cgagcugcuc     2700

aucagccacc acaccccgga caacaugccc aagcaguccc gcaagcggcu gcgcgugcgg     2760

ggcaaggucc agcgcaacga ccggggcgag gugaaguaca ugcaggggga cacggcccgc     2820

ggcgcccugc accagcagac cuucuacggg gccaucgagc gggacggcga gauccgcuac     2880

gugguccgca agagccucga ccagcugcug cccgccgacg uggagaagau cguggacgag     2940

gcgguccggc agaaggugcg cgaggccgug gaggccuacg gguucaagga ggccaccgac     3000

cccaccaagc acacggucug gaugaacgag gagaagcaga ucccgauccg gaaggugcgc     3060

auccucaccg gcgugaagaa ccccauccug cugaagcccc accgggacgu cuccgugaag     3120

gaguacaagc gcuacuacca cguggucaac gacggcaacu acugcauggc caucuacgag     3180

gggcgggacc gccagggccg gcucaagcgc accuucgaga ucgugaacaa gcuggaggcg     3240

gcccgguacu ucaagcgcag cgccgaccgg gagucccgcc ccgaccuggu gccgcucagc     3300

gacgucaacg gguacucccu gaaguaccug cucaagaccg gcaccauggu gcuguucuac     3360

gagaacagcc ccgacgagcu guacgagugc agcguggggg agcucgccaa gcggcuguac     3420

aaggucgugg gcauggccca ggacggccgc gugcaguucc uguuccacca ggaggcgcgg     3480

gaccagaagg ccgucacggc cgagugcggg augggcaucu ccguguucga cgcccgccac     3540

cccgcgccca agcuccggau ccgcgugagc aacuucaaga uguucgucga gggguacgac     3600

uucgagcuga ccgugaccgg cgaggugaag uucaagcggu ga                        3642


<210>  1722
<211>  3642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1722
auggagaagc gccugggcau cgacgugggg accaacagcc ucggcugggc caucgucgag       60

guggacgacg aggggugcac ccugcuggac cggggcgugg acaucuucca ggaggggguc      120

gcccgcgaga agaaccagga gaagcccgcg gugcaggacc ggacggacgc ccgcgcccuc      180

cggcgccacu acuuccggcg ccggcugcgc aagaucgccc ugcuccgggu gcugguccgc      240

cacgaccugu gcccggcccu cuccgaggag cagcugaacg cguggcaggg caagggcauc      300

uacccccugg acgacgccuu cauccggugg cagcgcaccg acgacaacgc cgaccggaac      360

cccuaccgcg accgguuccg cugccucacc gaggagcugg accuggccug ccgggaggag      420

cgcuacgugc ucgggcgggc gcuguaccac cugugccagc gccgcggcuu ccucagcaac      480

cggaaggacg ccgggaacga ggccgaggag ggcaagguga agcaggagau caagacccug      540

uccgccgaca uggccgcggc cgggugcgag uaccugggcg aguacuucua ccgccucuac      600

cagcggaagg agaagauccg caagcacuac acggcccgga acgagcacua ccuggccgag      660

uuccgcgcca ucugccggaa gcagcgccug gacgaggagc uccggcaggc gcuggagaag      720

gccaucuucu accagcgccc ccucaagagc cagaagggcc ucgucgggaa gugcaccuuc      780

gagaaggaca agucccggug cccggugagc cacccccgcu ucgaggaguu ccggaugcug      840

caguucauca acaacgugcg cgucaccggc cccggggacg ccgagccccg gccgcugucc      900

cccgaggagg uggagaucgu gcgcccccuc uuccugcgga aguccaagcu gcacuucgac      960

uucgaggaca ucgccaagaa gaucgccggc aagggcaacu acgcgugccg cggggagcuc     1020

cugcaggccc ccuaccgguu caacugcgcc ggcaagagca ccgucacggg gagcccggug     1080

uccgcccugu ucgccagcgu guucggcgac gacuggcagc gcgagaucug cucccucuac     1140

acccuggggg cgggcaagaa cgaggagcag auccugaacg acgucuggca cgugcuguuc     1200

gccuucgagg acgaggagcg ccugcagacc uggcugcagg agaagcucca gcugagcgcc     1260

gacgacgcca agacccuggc ggccuuccgg cucccccagg gcuacgccuc ccugagccug     1320

aacgugauca acaagauccu ccccuaccug cgccgggggu accgcuacga cgaggccguc     1380

uucuucgcca accugggcaa ggugcugccc gcggaggugu acgccgaccc ggcccggcgc     1440

cgggaggucg agcaggggau cuccgcccag auggacgacc ucggcgacgg ggagcagacg     1500

cgcaaggagg agcggauccg cgacuaccug cggcgcuacu ggggcgugga ggagaagcgg     1560

cuggagcgcc ucuaccaccc cagcaugauc gaggccuacc ccuccgcgag ccccaacggc     1620

caggggcugc ugcagcucgg cagcccgcgg auccccgccg uccgcaaccc cauggccaug     1680

cgggcccugu uccgccugcg ggugcuccug aacgagcugc uccgcgaggg gaagaucgac     1740

cggggcacca agaucaacau cgagcuggcc cgcgagcuga acgacgcgaa caagcgcaag     1800

gccaucgagc gguaccagcg cgagcgggag gccgagaacc gcaaguacgc cgaggagauc     1860

cggcagcagu acguggaggc gaccgggcac uccaucgagc ccagcgagga ggacauccuc     1920

aaguaccgcc ugugggucga gcagggccac ugcucccugu acaccggcaa gacgaucggg     1980

aucagcgagu uccucggcga cgggcggcgc uacgacaucg agcacaccgu gccgcggucc     2040

cgcggcggcg aggacgccca guacaacaag acccugugcg aggaggaguu caaccgggac     2100

aucaagcgcg acaagcugcc cagcgagcuc gccaaccaca ccgccauccu ggccugcgug     2160

gaguccugcg ggugguucga gcggaucgag ggccuggagc accgcgucug gggcgcggac     2220

cggcgcgcca agagcgccac gaccaagucc gccaaggacu ucgccaucca ggaccggcac     2280

uacuggcgca ugcaccucga cuacuggcgg cgcaagugcg aggcguucac caugaccgag     2340

gugcccgagg gguucagcaa ccgccagggg guggacgucg gcaucaucgg ccgguacgcc     2400

cgccuguacc ugcagacggu guucgaccgg guguacaccg ucaagggggc caccaccgcc     2460

gccuuccgcc ggaugugggg ccuccaggag gaguacgcgg ccaaggagcg cgugaaccac     2520

gugcaccacu gcaucgacgc caucacgauc gccugcaucg ggucccggga guacgcgcug     2580

ugggcccagu acguccgcca ggacgagcug uaccggcgcg gcgaggccga caagccccgg     2640

ugcgggaagc cguggcccac cuucaccgag gacgugcgcg ccaccgccga cgagcuccug     2700

aucagccacc acacgcccga caacaugccg aagcaguccc ggaagcgccu gcgggugcgc     2760

ggcaaggucc agcggaacga ccgcggcgag gugaaguaca ugcaggggga caccgcgcgg     2820

ggcgcccucc accagcagac cuucuacggg gccaucgagc gcgacggcga gauccgguac     2880

gugguccgca agagccugga ccagcugcuc cccgccgacg uggagaagau cguggacgag     2940

gccguccgcc agaaggugcg ggaggcggug gaggccuacg gguucaagga ggccaccgac     3000

cccacgaagc acaccgucug gaugaacgag gagaagcaga uccccauccg caaggugcgg     3060

auccugaccg gcgugaagaa cccgauccug cucaagcccc accgcgacgu cuccgugaag     3120

gaguacaagc gguacuacca cguggucaac gacggcaacu acugcauggc caucuacgag     3180

gggcgcgacc ggcagggccg ccugaagcgg accuucgaga ucgugaacaa gcuggaggcc     3240

gcgcgcuacu ucaagcggag cgccgaccgc gagucccggc ccgaccucgu gccccugagc     3300

gacgucaacg gguacucccu gaaguaccuc cugaagaccg gcacgauggu gcuguucuac     3360

gagaacagcc cggacgagcu cuacgagugc agcguggggg agcuggccaa gcgccuguac     3420

aaggucgugg gcauggccca ggacggccgg gugcaguucc ucuuccacca ggaggcccgc     3480

gaccagaagg cggucaccgc cgagugcggg augggcaucu ccguguucga cgcccggcac     3540

cccgccccca agcugcgcau ccgggugagc aacuucaaga uguucgucga gggguacgac     3600

uucgagcuga ccgugaccgg cgaggugaag uucaagcgcu ga                        3642


<210>  1723
<211>  3651
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1723
augaccaaga uccugggcau cgacaccggg acgaacagcc ucggcugggc caucguggac       60

aagaccgccg acaacuacaa ccugcuggac aaggggguca acaucuucca ggagggcgug      120

aagaucgaga aggggaucga guccagcaag gcggccgagc gcaccgagca ccgggccacc      180

cgcgugcggu acuaccgcau caagcuccgg aagauccgcc ugcugcggau ccucuccgac      240

aaccaccugu gcccgccccu gagccaggag gagcucucca cguggcgccu gaagaagguc      300

uaccccaaga acgagcuguu caugcagugg cagcggaccg acgacgacac cgagaagacc      360

cccuacgccu accgccacaa gugcauccac gagaagcucg accugagcga cgugacggac      420

cgguacaucc ugggccgcgc cuucuaccac aucauccagc ggcgcggcuu ccucuccaac      480

cggaaggagc agggcgggga ggacagcggg aaggugaagg agggcaucuc cacccugacc      540

cacgagauga acgaggcggg guacgaguac cugggcgacu acuucuacgc ccucuacaac      600

aagggcgaga agauccgcaa gcacuacacc gcccggaacg agcacuuccu gagcgaguuc      660

aaggccaucu gcgagaagca ggaccuggag aaggagcucg gguccgaggu cgugcgcaac      720

aucgagaagg cgaucuucga ccagcggccc cugaaguccc agaagggcca gguggggaag      780

ugcgucuucg agaagaacaa gacgaagugc ccguccagcc accccaugua cgaggaguuc      840

cgcaugcuga ccuucaucaa caacaucaag aucaagaccc ccaacgacga cgugcuccgg      900

ccccugaaca ccggcgagcg cgagaagauc augccgcugu ucuuccgcaa guccaagaag      960

caguucgacu ucgaggacau cacgaagaag cucgccccca agaagaugua cggcuucuac     1020

aagaaggagg aggacgccga gaugcccuuc cuguucaacu accccaugga caccagcgug     1080

uccgggugcc cggucaccgc cgcccugaag gacguguucg gcgacgacug gaucaccgcg     1140

aucugcgaga cguacacccu cggggagggc aagagccggu ucgacaucgu gaacgacauc     1200

uggcacgccc uguacuucua cuccgacaag gacaagcugg ccgaguucgc caucaaccgc     1260

cuccagcuga gcgaggacga ggccaagauc uucagcgaga ucucccugcc cgcggacuac     1320

gccagccucu cccugaaggc caucugcaag auccugcccu accuccggcg cgggcugauc     1380

uacagccacg ccgucuuccu gggcaaccuc ugcgagguga ugcccaagua cgaguggucc     1440

aucaaggaga ugcgggagac caucaucgac aacaucauca uggagaugga caagugcgac     1500

accaaggaca agcgcacguu cgaggugugc aucaaggacu accugaaggg ccaguacaac     1560

gucagcgaca acucccugaa gaagcucuac cacccgagca ugaucgagac cuacccccgg     1620

gugcaguaca ccaacgagca cgggguguac cagcugggca gcccccgcau cgacagcguc     1680

cggaacccca uggccaugcg cuccauguuc cggcugcgca agcucgugaa ccggcugcug     1740

guggagggga agaucgacca gaacaccgag guccacaucg aguucgcgcg cgagcugaac     1800

gacgccaaca agcggaacgc caucgcccuc uucgugaagg agaaccagag cgccaacgac     1860

gaggcgcgca agcagaucau cacgcuguuc aagaaggaga ccggcaccga caucgagccg     1920

accgacaagg acauccugaa guacguccuc ugggaggagc agaaccacau cugccuguac     1980

acggggaagc agauccggau cuccgacuuc aucggcgcca accccaaguu cgacaucgag     2040

cacaccaucc cccgcagcgu gggcggggac uccaccaaga ugaaccugac ccucugcgac     2100

agccgguuca accgcgaggu gaagaagacg aagcugcccg ucgagcugcc gaaccacgac     2160

gagaucaugg agcgcaucaa ggaguggaag aagaacuacg agcuccugga cauccagauc     2220

cggaagaaca agaagcuguc caagggcgcc accaccaagg agcagaagga caccaucauc     2280

cgcaagcggc accuccugga gcugcgccgg gacuacuggc gcgggaagua cgagcgguuc     2340

accauggaga gcgugcccga gggcuucucc cgccggcagg ggacggacau cagcgugauc     2400

agcaaguacg cccgccucua ccugaagucc cuguucaacc gggucuacac cgugaagggc     2460

aucgcgacca gcgacuuccg caagaucugg ggcauccagg acaucuacuc caagaaggag     2520

cggaucaacc acgugcacca cugcaucgac gccaucguca ucgccugcau cgggcucgac     2580

gaguacaaca agcugggcgc cuacuaccac gacgaggaga acaacaagug guacgggaug     2640

agcaaggccu ccuucaagaa gcccugggcg accuucgugg aggacaucaa gaagauccag     2700

gacgagaucc ugguguacca cuacacgccc gacaacaugc cgaagcaggg ccgccggcgc     2760

aucaagaucg acggcaagaa gauccucugc aagggggacg ccgcccgggg cagccugcac     2820

aacgacaccu acuacggggc caucgagaac gacggcgccg ucaaguacgu gaagcgcauc     2880

gaccuggcgu cccucgagga gaaggacgug aagaacaucg ucgacgacac cgugcggagc     2940

aucaucgagg ccgccaucaa ggagaagggg uucaaggacg ccauggccuc caccaucugg     3000

augaacgagg agaagcgcgu gcccaucaag aagguccggu gcuucacgcc cagcaucacc     3060

aagccgcuga acauccgcaa gcagcgcgac guguccauca aggaguacaa gcagcaguac     3120

cacguggcga acgacagcaa cuaccugcuc gcccuguaca ucggcaccga caacaagggc     3180

aaggagaagc gggaguucga gaucgucaac auccugcagg ccgcccagua cuaccgcacc     3240

uccaacgaca aggagguggu ggaccggcac aucgucccca ucaagagcga gcacgacuac     3300

cccuucgcgu acacgcucaa gaucgggacc auggugcugc uguacgagaa gucccccaac     3360

gagguguggg acgccaccau caaggagcgc aaccggcgcc ucuacaaggu caccggccug     3420

agcacgaugc ggaugaaggg gcgcaacggc gaguacgccu acgccaccgu gaagcugauc     3480

cacaacgagg aggcccggcc guccaaggac aucaaggcga agaacgggga guacgagcag     3540

ggcgaggagu uccgccccgc caucaucaug cuccacaccc agcugaacgc ccuggugcag     3600

ggcuacgacu ucgagaucaa cgagcucggg gagauccggc gccugcggug a              3651


<210>  1724
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1724
auggagaagc agcgcauccu gggccucgac cccgggacca acagccuggg cugggccgug       60

cuggacuucg acaacuccaa gcaggaguac aaccucgucc ggaaggggga ggugaucuuc      120

gaggagggcg ugggcaagag cgagcgcggg gaguucucca aggccgcgua ccggaccuuc      180

uacaagagca cgcgcaagca cuacuuccgg cgccggcugc gcaagaucga gguccugaag      240

gugcucguga aguacgacuu cugcccguac cuguccgacg accugcucaa gcaguggcac      300

gagaagaaga ucuaccccgc cgucaaggag uucauggacu ggcagcggac cagcgacgcc      360

gagggcaaga accccuacua cgaccgccac ugcugccuga acgagaagcu ggaccucucc      420

cucaagagcg accgguacau ccuggggcgc gcccuguacc accugaccca gcggcgcggc      480

uucaagucca accggcugau ccagaacaac gacgagaacg aggaggggaa ggugaagaag      540

ggcaucagcg agcucuccac cgagaugcag gaggccaacu gcaacuaccu gggggacuac      600

uucuacaagc uguacacgga gaagggcaac accgugcaga uccgcaagcg guacaccgac      660

cgcgagaagc acuacaagaa cgaguucaac gcgaucugcg agaagcagga gcucaacgag      720

accaucguca aggagcugga caaggccauc uucuuccagc ggaagcugaa gccccagcgc      780

cacggcgugg ggaagugcac guucgagccg aacaaccggc gcugcgccau cagccacccc      840

gacuucgagc gcuuccggau gcucugcuuc aucaacaaca ucaagaucaa gacccccuuc      900

gaggagaacc ugcgcccccu gaacaacgag gagaaggccc ucaucgagcc gcuguucuac      960

cggaagucca ucaagaacuu cgacuucgag gagaucgcga agaagcuggc cggcaagaac     1020

aacuacugcu acaucaagga cgacagcucc aagcaguaca aguucaacua ccgcaugagc     1080

cagggggccu ccacgugcca gaccaucgcc aagcucaaga gcaucuucgg cgaggagugg     1140

aagaacggca ucgccgagac guacaccaac cggcgcaaga aggaggggcu gaagaagcag     1200

gcggagaugg uggacgacau cuggaacguc cuguacuccg ugagcgagug cgacgugcuc     1260

aaggacuucg ccaucaacaa gcugcagcug gacgagaaga ccgccgacaa cuucagcaag     1320

accaagcucg cccagggcuu cgccacgcug ucccugaagg cgauccggaa gauccucccc     1380

uuccuggagc agggguucgg cuacaccgag gccgucuucc uggccaacau ccccaccgug     1440

guggggaagg aguacuggaa caacgaggac auccgcaacg acaucaugga ggagaucaag     1500

accaucuucu accacaacac gcucaagacc aacgacagcc acaacaccau ccuguucugc     1560

aucaaggaca uccuguccaa caacuucgac cucgcccccg gcgccaucga caagcuguac     1620

cacccgagca ugaucaacgu cuacaaggac gugaucccca acgaggacgg cauccugcag     1680

cucggguccc ccaagaucga cgcggugaag aaccccaugg ccaugcggag ccugcacauc     1740

auccgcaagg ucgugaacga gcugcugcgg gagggcauca ucaacuccga caccgaggug     1800

cgcaucgagu acgcccggga gcucaacgac gccaacaagc gccacgccau cgcggacugg     1860

cagaagaagc aguacgggaa gcaccagaag uaccggaagg agaucaagga gcuguucaag     1920

gccgagacgg gcuucgagau cgagccgaac gagaccgaca uccugaaguu cgagcucugg     1980

gaggagcaga accacaucug ccuguacacc gggcaggaga ucggcaucag cgaguucguc     2040

ggcgagaacc ccaaguacga caucgagcac accuuccccc gcucccucgg gggcgacagc     2100

acgaagauga acaugacccu gugcgagaac aaguucaacc gggagaacaa gcagaagaag     2160

cugcccuccg agcuggccaa ccacagcgag auccucgagc gcaucgacga cuggaagaag     2220

aagaucuaca uccugcagaa gaaguacgac uccauccgga cccacaacgg gauggacaag     2280

gagaacaagg acaagaagau cgugagccgc cacaagauca agcuggagcu cgacuacugg     2340

aagggcaagu acaaccgcuu caccaugacg gagaucccgg agggguucuc ccggcgccag     2400

ggcaugggca ucgggcugau cagccgguac gccggccugu accucaagac caucuuccac     2460

aagaaggaca acccccagaa guccaacguc uacaucguga aggggagcau caccgcggag     2520

uuccgccgga ucuggaacau ccaggacgac uacgagaaga agugccgcga caaccacauc     2580

caccacugca ucgacgccau cguggucggc ugcaucggca agcgggagua cgaccugaug     2640

gcccaguucu accgcgacga ggagaaguac cggugggaga agaagaagaa gcccuucuuc     2700

aagaagcccu gggagaccuu cacgcaggac augcuguccc ucaaggacga ggugcugacc     2760

gugcacuuca acccgagcaa cuucaccaag aaggcccaca agaaggucuu cacccccaag     2820

gggaucuucg uggcccaggg cgacugcgcg cgcgugaagc ugcacaaggg gagcuacuuc     2880

ggcgccaucg agcagaaggg ggagaucaag uacgucaugc ggaaggagcu cuccgcccug     2940

aagaucgagg acaucaagaa caucguggac gccgugguca aggagaaggu gcuggccgug     3000

gucaagaaga agggcuucaa gcaggcgaug gccgagccca ucuacaugaa cgaggacaag     3060

cgcauccuca ucaagaaggu gcggcuguuc gugagccagg ccaacccccu gaucgucaag     3120

aagcaccgcg accucuccag caaggaguac aagcagaacu acuacgugga caacgagggc     3180

aaccugauga ucgccaugua cgagggggug aagaagaacg gcaagaucga ccgggagauc     3240

accgucguga acaaccugga ggccgcgaag uucuuccgcc agucccagaa gaacaacgcc     3300

gagaagcagc ucaucagcca ccuguccccg aagaacgggu acccccugaa gacggugcuc     3360

acccagaagc agcugguccu gauguacgag gacagcccca aggagaucaa gcuccgggac     3420

accaagaaca uggugaagcg ccuguaccag guguucggca ucgagaagga cgggcggguc     3480

aagcugaagu uccaccagga ggcccgcucc gagggccuca acaucaacag cgccgcguuc     3540

aagauccagg acaccccgga gucccuguac cggcacacca agagcaaccu gaaggugcuc     3600

gugaacggcg ucgacuucaa gaucaacauc cugggggaga ucucccugau cuga           3654


<210>  1725
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1725
auggagaagc agcgcauccu gggccucgac cccgggacca acagccuggg cugggccgug       60

cuggacuucg acaacuccaa gcaggaguac aaccucgucc ggaaggggga ggugaucuuc      120

gaggagggcg ugggcaagag cgagcgcggg gaguucucca aggccgcgua ccggaccuuc      180

uacaagagca cgcgcaagca cuacuuccgg cgccggcugc gcaagaucga gguccugaag      240

gugcucguga aguacgacuu cugcccguac cuguccgacg accugcucaa gcaguggcac      300

gagaagaaga ucuaccccgc cgucaaggag uucauggacu ggcagcggac cagcgacgcc      360

gagggcaaga accccuacua cgaccgccac ugcugccuga acgagaagcu ggaccucucc      420

cucaagagcg accgguacgu gcuggggcgc gcccuguacc accugaccca gcggcgcggc      480

uucaagucca accggcugau ccagaacaac gacgagaacg aggaggggaa ggugaagaag      540

ggcaucagcg agcucuccac cgagaugcag gaggccaacu gcaacuaccu gggggacuac      600

uucuacaagc uguacacgga gaagggcaac accguccaga uccgcaagcg guacaccgac      660

cgcgagaagc acuacaagaa cgaguucaac gcgaucugcg agaagcagga gcucaacgag      720

accaucguga aggagcugga caaggccauc uucuuccagc ggaagcugaa gccccagcgc      780

cacggcgugg ggaagugcac guucgagccg aacaaccggc gcugcgccau cagccacccc      840

gacuucgagc gcuuccggau gcucugcuuc aucaacaaca ucaagaucaa gacccccuuc      900

gaggagaacc ugcgcccccu gaacaacgag gagaaggccc ucaucgagcc gcuguucuac      960

cggaagucca ucaagaacuu cgacuucgag gagaucgcga agaagcuggc cggcaagaac     1020

aacuacugcu acaucaagga cgacagcucc aagcaguaca aguucaacua ccgcaugagc     1080

cagggggccu ccacgugcca gaccaucgcc aagcucaaga gcaucuucgg cgaggagugg     1140

aagaacggca ucgccgagac cuacaccaac cggcgcaaga aggaggggcu gaagaaccag     1200

gcggagaugg ucgacgacau cuggaacgug cuguacuccg ugagcgagug cgacguccuc     1260

aaggacuucg ccaucaacaa gcugcagcug gacgagaaga ccgccgacaa cuucagcaag     1320

accaagcucg cccagggcuu cgccacgcug ucccugaagg cgauccggaa gauccucccc     1380

uuccuggagc agggguucgg cuacaccgag gccguguucc uggccaacau ccccaccgug     1440

gucgggaagg aguacuggaa caacgaggac auccgcaacg acaucaugga ggagaucaag     1500

accaucuucu accacaacac gcucaagacc aacgacagcc acaacaccau ccuguucugc     1560

aucaaggaca uccuguccaa caacuucgac cucgcccccg gcgccaucga caagcuguac     1620

cacccgagca ugaucaacgu guacaaggac gugaucccca acgaggacgg cauccugcag     1680

cucggguccc ccaagaucga cgcggucaag aaccccaugg ccaugcggag ccugcacauc     1740

auccgcaagg uggugaacga gcugcugcgg gagggcauca ucaacuccga caccgagguc     1800

cgcaucgagu acgcccggga gcucaacgac gccaacaagc gccacgccau cgcggacugg     1860

cagaagaagc aguacgggaa gcaccagaag uaccggaagg agaucaagga gcuguucaag     1920

gccgagacgg gcuucgagau cgagccgaac gagaccgaca uccugaaguu cgagcucugg     1980

gaggagcaga accacaucug ccuguacacc gggcaggaga ucggcaucag cgaguucgug     2040

ggcgagaacc ccaaguacga caucgagcac accauccccc gcucccucgg gggcgacagc     2100

acgaagauga acaugacccu gugcgagaac aaguucaacc gggagaacaa gcagaagaag     2160

cugcccuccg agcuggccaa ccacagcgag auccucgagc gcaucgacga cuggaagaag     2220

aagaucuaca uccugcagaa gaaguacgac uccauccgga cccacaacgg gauggacaag     2280

gagaacaagg acaagaagau cgucagccgc cacaagauca agcuggagcu cgacuacugg     2340

aagggcaagu acaaccgcuu caccaugacg gagaucccgg agggguucuc ccggcgccag     2400

ggcaugggca ucgggcugau cagccgguac gccggccugu accucaagac caucuuccac     2460

aagaaggaca acccccagaa guccaacgug uacaucguga aggggagcau caccgcggag     2520

uuccgccgga ucuggaacau ccaggacgac uacgagaaga agugccgcga caaccacauc     2580

caccacugca ucgacgccau cgucgugggc ugcaucggca agcgggagua cgaccugaug     2640

gcccaguucu accgcgacga ggagaaguac cggugggaga agaagaagaa gcccuucuuc     2700

aagaagcccu gggagaccuu cacgcaggac augcuguccc ucaaggacga ggugcugacc     2760

guccacuuca acccgagcaa cuucaccaag aaggcccaca agaagguguu cacccccaag     2820

gggaucuucg uggcccaggg cgacugcgcg cgcgucaagc ugcacaaggg gagcuacuuc     2880

ggcgccaucg agcagaaggg ggagaucaag uacgugaugc ggaagaagcu cuccgcccug     2940

aagaucgagg acaucgagca caucguggac gccgucguga aggagaaggu gcuggccguc     3000

gugaagaaga agggcuucaa gcaggcgaug gccgagccca ucuacaugaa cgaggacaag     3060

cgcauccuca ucaagaaggu gcggcuguuc gucagccaga ccaacccccu gaucgugaag     3120

aagcaccgcg accucuccag caaggaguac aagcagaacu acuacgugga caacgagggc     3180

aaccugauga ucgccaugua cgaggggguc aagaagaacg gcaagaucga ccgggagauc     3240

acggugguga acaaccugga ggccgccaag uucuuccgcc agucccagaa gaacaacgcg     3300

gagaagcagc ucaucagcca ccuguccccg aagaacgggu acccccugaa gaccguccuc     3360

acccagaagc agcuggugcu gauguacgag gacagcccca aggagaucaa gcuccgggac     3420

accaagaaca uggugaagcg ccuguaccag gucuucggca ucgagaagga cgggcgggug     3480

gagcugaagu uccaccagga ggcccgcucc gagggccuca acaagaacag cgccgccuuc     3540

aagauccagg acacgccgga gucccuguac cggcacacca agagcaaccu gaaggugcuc     3600

gucaacggcg uggacuucaa gaucaacauc cugggggaga ucucccugau cuga           3654


<210>  1726
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1726
augaaguaca acuacgugcu gggccucgac aucgggauca gcuccgucgg cugggggcug       60

cuggcccucg acgagaacaa caaccccuac aagaucaucg acgugggcag ccgcaucuuc      120

uccccggggg agguggagaa gaccggcgac agcaaggcca aggagcggcg cgagaagcgg      180

ggcacccgcc gggucauccg ccggcgcgag uuccggcugg accgcgugcg gaaccugcuc      240

uacgagaagg gguaccugaa gggcaacauc acguccaaca ucgugagcgu caagaacgag      300

gagcugacca ucaucuucaa cgacauggug aacaacuacu acaaguccaa ggacaccaac      360

cccuacaagc ucaaggugga ggcgcuggac cgcaagcuga ccaacgacga gcucagcauc      420

auccuggucc acuacgccaa gaagcggggg uacaagucca accgcgagga gagcuccgac      480

aacgacaacg gcaaggugaa ggaggccauc aaggagaaca cgaaccugau ggaggagaag      540

aaguaccgga ccaucagcga gauguacauc aagaacgaga aguucaagaa caagaucaag      600

aacgggcccg acgacuacaa gcucuccgug accaacgaga uguaccugga ggagaucaac      660

aagguccugg acagccagau cggcuucggc cucaucgacg acaacuucaa gaacgaguac      720

cuggagaucu acaacucccg ccggcacuac agcgaggggc ccggcuacua cuacgccaag      780

gacgccaacg ggaacaaggu gaaggugcgc uccccguacg gcggcgaccu gaucaagaag      840

augaccgggg ucugcagcuu cgacaagaag gcgcgggccc ccaaguacgc cuucuccagc      900

gagcuguucg ugagcaucuc caagcuggug aaccugcgcu acaagaucga cgacgacagc      960

uacaagcagc ucacgaagga gcagaucaac gagcugaucg acgaggccaa gaacaagaac     1020

aaggucaccu acaagucccu gaucaagauc cucggcauca agaacaucaa ggugaaggac     1080

cugaaccuga gcaaguccga guacaucaag gugaucgacg agcucaagaa gaagcugaac     1140

aucagcaagg aggagaagau cgacaucuac ucccuggacg aggagaacaa gaagaucuac     1200

gacgacaucu acaacgagaa gcuguucagc aagacccuca ucgaguucaa gggguacaac     1260

cugcucaaga aggcgaucac ccuguccuac aacagcggca cguggaacga cgucaaggac     1320

aacaucgagu uccuggacga gcucgcccug uacugcaccg acuacaaggu gaacaacgag     1380

auccaggagc acauccacaa cucccugauc aucgacagcc gguucgacga cuccaacuuc     1440

guggagaacc uccccaacuu caaggaccac cugaugcuga gcaccgacau cauccgcaag     1500

cucaucccca ucaugcugga ggggaucacc uacgacaagg ccauggacaa gaucggcuac     1560

aaccacucca accuguacaa ggacacgauc aaggaggacc uccugguccc gguguacguc     1620

agcaagaaca ucaccaacca gcgcgugauc cggucccuga cccaggcccg caaggugauc     1680

aacgccauca ucaagaagua cggcagcccc aagaucauca acaucgagac cgcgcgggag     1740

cuggccaaga cgcagaagga gcgccggauc aucgagaaga accagcagga caagcaguac     1800

gagaacgagc gcaucaagaa gcaccucguc gagcuggagc uguucuccga cgagagcaag     1860

aucaccggga acgaccuccu gaaguacaag cuguggcagg agcagaagga guucugcggc     1920

uacagcauga agaagaucaa gcucgaggac cuguucucca acaacaucgu gcagaucgac     1980

cacauccugc ccuacagccg gaccuacaac gacaacuacc ucaacaagac ccugguguac     2040

acgaaguaca accaggacaa ggggaacaag acccccuacg agugguucgg caagaccgac     2100

aaguggcagg aguacgaggc cuucaucaac ucccugagca ucucccagaa caagaaggac     2160

aacuaccucc ugaagaaccu ggaccaggac gccgagcgcg agaugcggga gcagaaccuc     2220

aacgacacca aguacaucag ccgcgagcug cugucccuca ucaaggccua ccugaacguc     2280

gacgagguga acgcguuccc gggggccauc acggccaagc ugcgggcccg cuggggcuuc     2340

aaccggcuca cccacagcua caucuccaag accuacauca ugcccgagga caugaagaag     2400

ggcaucaaca aggaccgcga caaccaccuc caccacgcca uggacgcgcu ggugaucgcc     2460

agcaucacca agucccucca gcagaagauc acgcuguacg agaaguucag ccgguacguc     2520

gacgggauca ccaagaacga gcugggcaag aucuccaccg ggaacaucaa cgacgaccuc     2580

uacaacuacu acgacgagga gaccggcgag aucaaggagc uggagcugcg ccaguacauc     2640

aagaaccagg uggcccagaa caacaucuac uucagcaagc acaacguguc caagcucauc     2700

uuccccacgc ccuacaacaa cuucgucgag gaggccaagc ugcgggugua cgagcgcgac     2760

cuggaggugc ucaaguggaa ccugaagaac cuggggaccu acaccagcuc cgagcucaac     2820

cggauccacg uccugauccc gagcguggcc aagaacaaga ucagcggccg ccugcacgag     2880

gagaccuacu acggccucaa ggaggugggg aucggcgagg acaaggucac cuacaagacg     2940

cugaagaccc ccaucuccaa ggugaagaag aaggaccugg acaacauccc cgacugcaag     3000

ggcgggagca aggacaucua caacgcgcuc gugaaguggu ucggcaacug cgagaccggg     3060

gaggaggccc ugaagaugca cgacggcaag uaccccguca acccgaacga caaggagcag     3120

aaggagaucc gcaagaucaa gguguacacc aaguacaacg ccacggggca cguggucaac     3180

aacggcaacg uggagaaggg cgggaucuac cggaucgacg uguucaaguc caagaacaag     3240

gaggacaaca agcuguacuu caccagcuac gacaucuucg agaucgagca gaucaaggcc     3300

uacgagaagg gcaagaacau caacuucucc aucaagcucg aguacgggca gggcaagaac     3360

aacaagaucc ugcuguacaa ggacgucaag aacgaguacg accucaucau gagcaucuac     3420

aagaacgacc ugauccgcau caagaccaag accgggaaca aguccacggc guacguggug     3480

ggcuucagcg ggggcaagcu ggagaucaag uccaccaucg gcgacgggua cgacaucauc     3540

ggcaacaaca acaucuucga caagagcaug gagcgguacc gcgucaccuc cagcaccauc     3600

gugaagaucg acaagcucuc caucagcauc cugggggagg uguccggccu guga           3654


<210>  1727
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1727
augaacaagc gcauccuggg ccucgacacc gggaccaaca gccugggcug ggccgugguc       60

gacugggacg agcacgccca guccuacgag cugaucaagu acggggacgu gaucuuccag      120

gagggcguga agaucgagaa ggggaucgag agcuccaagg cggccgagcg gagcggcuac      180

aaggccaucc gcaagcagua cuuccggcgc cggcuccgca agauccaggu ccugaaggug      240

cuggugaagu accaccucug ccccuaccug uccgacgacg accugcggca guggcaccuc      300

cagaagcagu accccaagag cgacgagcug augcuguggc agcgcacguc cgacgaggag      360

ggcaagaacc cguacuacga ccggcaccgc ugccuccacg agaagcugga ccugaccguc      420

gaggccgacc gguacacccu cgggcgcgcc cuguaccacc ugacccagcg gcgcggcuuc      480

cucagcaacc ggcuggacac guccgcggac aacaaggagg acgggguggu caagagcggc      540

aucucccagc ugagcaccga gauggaggag gccgggugcg aguaccucgg cgacuacuuc      600

uacaagcugu accacgagca gggcaacaag gugcgcaucc ggcagcgcua caccgaccgg      660

aagaagcacu accagcacga guucgacgcc aucugcgaga agcaggagcu guccagcgag      720

cucaucgagg accugcagcg cgccaucuuc uuccagcugc cccucaaguc ccagcggcac      780

ggggugggcc gcugcaccuu cgagcgcggg aagccccggu gcgcggacag ccacccggac      840

uacgaggagu uccgcaugcu gugcuucgug aacaacaucc aggucaaggg cccccacgac      900

cuggagcucc ggccccugac guacgaggag cgcgagaaga ucgagcaccu guucuuccgg      960

aaguccaagc ccaacuucga cuucgaggac aucgccaagg agcucgccgg caagaagaac     1020

uacgccugga uccacgacaa ggaggagcgc gccuacaagu ucaacuaccg gaugacccag     1080

ggggugccgg gcugccccac caccgcgcag cucaagagca ucuucgggga cgacuggaag     1140

acgggcaucg ccgagaccua cacccugauc cagaagaaga acggguccaa gagccugcag     1200

gagauggugg acgacgucug gaacgugcug uacagcuucu ccagcgugga gaagcugaag     1260

gaguucgccc accacaagcu ccagcuggac gaggagaacg ccgagaaguu cgccaagauc     1320

aagcuguccc acagcuucgc ggcccucucc cugaaggcca uccgcaaguu ccugcccuuc     1380

cuccggaagg gcauguacua cacccacgcc agcuucuucg ccaacauccc cacgaucguc     1440

ggcaaggaga ucuggaacaa cgagcagucc cgcaaguaca ucauggagaa cgugggggag     1500

cugguguuca acuaccagcc gaagcaccgg gacguccagg gcaccaucga gaugcugauc     1560

aaggacuucc ucgcgaacaa cuucgagcug cccgccgggg ccaccgacaa gcuguaccac     1620

cccagcauga ucgagaccua cccccacgcc cugcgcaacg aguucggcau ccuccagcug     1680

ggguccccgc ggacgaacgc cauccgcaac cccauggcga ugcggagccu gcacgugcuc     1740

cgccgggugg ucaaccagcu gcugaaggag uccaucaucg acgagaacac cgaggugcac     1800

gucgaguacg cccgcgagcu caacgacgcc aacaagcggc gcgccaucgc ggaccgccag     1860

aaggagcagg acaagcagca caagaaguac ggcgacgaga uccggaagcu guacaaggag     1920

gagaccggca aggacaucga gcccacccag acggacgugc ugaaguucca gcucugggag     1980

gagcagaacc acuacugccu guacaccggg gagcagaucg gcaucaccga cuucaucggg     2040

agcaacccca aguucgacau cgagcacacc aucccgcagu ccgugggcgg ggacagcacg     2100

cagaugaacc ugacccucug cgacaaccgc uucaaccggg aggucaagaa ggccaagcug     2160

cccaccgagc uggccaacca cgaggagauc cucacccgca ucgagcccug gaagaacaag     2220

uacgagcagc uggugaagga gcgggacaag cagcgcacgu ucgccggcau ggacaaggcc     2280

gugaaggaca uccggaucca gaagcgccac aagcugcaga uggagaucga cuacuggcgg     2340

ggcaaguacg agcgcuucac caugaccgag guccccgagg gguucucccg gcgccagggc     2400

accgggaucg gccucaucag ccgguacgcg ggccuguacc ugaagucccu cuuccaccag     2460

gccgacagcc gcaacaaguc caacguguac guggucaagg ggguggccac ggccgaguuc     2520

cggaagaugu ggggccugca gagcgaguac gagaagaagu gccgcgacaa ccacagccac     2580

cacugcaugg acgccaucac caucgcgugc aucgggaagc gggaguacga ccugauggcc     2640

gaguacuacc acauggagga gaccuuccgc cagggccggg gguccaagcc gaaguucagc     2700

aagcccuggg ccaccuucac cgaggacgug cucaacaucu acaagaaccu gcuggucgug     2760

cacgacacgc ccaacaacau gcccaagcac accaagaagu acgugcagac cuccaagggc     2820

aagguccucg cccagggcga caccgcccgc gggagccugc accuggacac guacuacggc     2880

gcgaucgagc gggacgggga gauccgcuac guggugcgcc ggccgcucuc cagcuucacc     2940

aaccccaagg agcuggagaa caucgucgac gagaccguga agcgcaccau caaggaggcc     3000

aucgccgaca agaacuucaa gcaggccauc gcggagccca ucuacaugaa cgaggacaag     3060

ggcauccuga ucaagaaggu gcggugcuuc gccaacuccg ucaagcagcc gaucaacauc     3120

cgccagcacc gggaccucag caagaaggag uacaagcagc aguaccacgu gaugaacgag     3180

aacaacuacc ugcuggccau cuacgagggg cucgugaaga acaaggucgu gcgcgaguuc     3240

gagaucgugu ccuacaucga ggccgccaag uacuacaagc ggagccagga ccgcaacauc     3300

uucuccagca ucguccccac gcacuccacc aaguacggcc ugccccugaa gaccaagcuc     3360

cugaugggcc agcagguccu gauguucgag gagaaccccg acgagaucca gguggacaac     3420

accaaggacc ucgugaagcg gcuguacaag guggugggga ucgagaagga cggccgcauc     3480

aaguucaagc accaccagga ggcgcggaag gaggggcuga cgaucuacag caccccguac     3540

aagaacaacg acgacuacgu ccccaucuuc cgccagucca ucaagaacau caacauccuc     3600

guggacggca ucgacuucaa caucgacauc cuggggaagg ugacccugaa ggaguga        3657


<210>  1728
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1728
augaacaagc gcauccuggg ccucgacacc gggaccaaca gccugggcug ggccgugguc       60

gacugggacg agcacgccca guccuacgag cugaucaagu acggggacgu gaucuuccag      120

gagggcguga agaucgagaa ggggaucgag agcuccaagg cggccgagcg gagcggcuac      180

aaggccaucc gcaagcagua cuuccggcgc cggcuccgca agauccaggu ccugaaggug      240

cuggugaagu accaccucug ccccuaccug uccgacgacg accugcggca guggcaccuc      300

cagaagcagu accccaagag cgacgagcug augcuguggc agcgcacguc cgacgaggag      360

ggcaagaacc ccuacuacga ccggcaccgc ugccuccacg agaagcugga ccugaccguc      420

gaggccgacc gguacacccu cgggcgcgcc cuguaccacc ugacccagcg gcgcggcuuc      480

cucagcaacc ggcuggacac guccgcggac aacaaggagg acgggguggu caagagcggc      540

aucucccagc ugagcaccga gauggaggag gccgggugcg aguaccucgg cgacuacuuc      600

uacaagcugu acgacgccca gggcaacaag gugcgcaucc ggcagcgcua caccgaccgg      660

aacaagcacu accagcacga guucgacgcc aucugcgaga agcaggagcu guccagcgag      720

cucaucgagg accugcagcg cgcgaucuuc uuccagcugc cccucaaguc ccagcggcac      780

ggggugggcc gcugcaccuu cgagcgcggg aagccccggu gcgccgacag ccacccggac      840

uacgaggagu uccgcaugcu gugcuucgug aacaacaucc aggucaaggg cccccacgac      900

cuggagcucc ggccccugac guacgaggag cgcgagaaga ucgagccccu guucuuccgg      960

aaguccaagc cgaacuucga cuucgaggac aucgccaagg cccucgccgg caagaagaac     1020

uacgcgugga uccacgacaa ggaggagcgc gccuacaagu ucaacuaccg gaugacccag     1080

ggggugcccg gcugccccac caucgcccag cucaagagca ucuucgggga cgacuggaag     1140

accggcaucg ccgagacgua cacccugauc cagaagaaga acggguccaa gagccugcag     1200

gagauggugg acgacgucug gaacgugcug uacagcuucu ccagcgugga gaagcugaag     1260

gaguucgccc accacaagcu ccagcuggac gaggaguccg cggagaaguu cgccaagauc     1320

aagcugagcc acuccuucgc cgcccucagc cugaaggcca uccgcaaguu ccugcccuuc     1380

cuccggaagg gcauguacua cacccacgcg uccuucuucg ccaacauccc gaccaucguc     1440

ggcaaggaga ucuggaacaa ggagcagaac cgcaaguaca ucauggagaa cgugggggag     1500

cugguguuca acuaccagcc caagcaccgg gagguccagg gcacgaucga gaugcugauc     1560

aaggacuucc ucgccaacaa cuucgagcug cccgccgggg ccaccgacaa gcuguaccac     1620

cccagcauga ucgagaccua cccgaacgcg cagcgcaacg aguucggcau ccugcagcuc     1680

ggguccccgc ggaccaacgc cauccgcaac cccauggcca ugcggagccu gcacauccug     1740

cgccgggugg ugaaccagcu ccugaaggag uccaucaucg acgagaacac ggagguccac     1800

guggaguacg cccgcgagcu gaacgacgcg aacaagcggc gcgccaucgc cgaccgccag     1860

aaggagcagg acaagcagca caagaaguac ggcgacgaga uccggaagcu cuacaaggag     1920

gagaccggca aggacaucga gcccacccag accgacgucc ugaaguucca gcugugggag     1980

gagcagaacc accacugccu cuacacgggg gagcagaucg gcaucaccga cuucaucggg     2040

agcaacccga aguucgacau cgagcacacc aucccccagu ccgugggcgg ggacagcacc     2100

cagaugaacc ugacgcugug cgacaaccgc uucaaccggg aggugaagaa ggccaagcuc     2160

cccaccgagc uggccaacca cgaggagauc cugacccgca ucgagcccug gaagaacaag     2220

uacgagcagc ucgucaagga gcgggacaag cagcgcaccu ucgcgggcau ggacaaggcc     2280

gugaaggaca uccggaucca gaagcgccac aagcugcaga uggagaucga cuacuggcgg     2340

ggcaaguacg agcgcuucac gaugaccgag gugccggagg gguucucccg gcgccagggc     2400

accgggaucg gccugaucag ccgguacgcc ggccucuacc ugaagucccu guuccaccag     2460

gccgacagcc gcaacaaguc caacgucuac guggugaagg gggucgccac cgcggaguuc     2520

cggaagaugu ggggccucca gagcgaguac gagaagaagu gccgcgacaa ccacagccac     2580

cacugcaugg acgccaucac gaucgccugc aucgggaagc gggaguacga ccugauggcc     2640

gaguacuacc gcauggagga gaccuucaag cagggccggg gguccaagcc caaguucagc     2700

aagcccuggg ccaccuucac cgaggacgug cugaacaucu acaagaaccu ccuggugguc     2760

cacgacaccc ccaacaacau gccgaagcac acgaagaagu acgugcagac cuccaucggc     2820

aaggugcugg cgcagggcga caccgcccgc gggagccucc accuggacac cuacuacggc     2880

gccaucgagc gggacgggga gauccgcuac gucgugcgcc ggccccuguc cagcuucacg     2940

aagcccgagg agcucgagaa caucguggac gagaccguca agcgcaccau caaggaggcc     3000

aucgcggaca agaacuucaa gcaggccauc gccgagccga ucuacaugaa cgaggagaag     3060

ggcauccuga ucaagaaggu gcggugcuuc gccaaguccg ugaagcagcc caucaacauc     3120

cgccagcacc gggaccugag caagaaggag uacaagcagc aguaccacgu caugaacgag     3180

aacaacuacc uccuggccau cuacgagggg cuggugaaga acaagguggu ccgcgaguuc     3240

gagaucgugu ccuacaucga ggcggccaag uacuacaagc ggagccagga ccgcaacauc     3300

uucuccagca ucgugcccac ccacuccacg aaguacggcc ucccccugaa gaccaagcug     3360

cucaugggcc agcugguccu gauguucgag gagaacccgg acgagaucca gguggacaac     3420

accaaggacc ucgugaagcg gcuguacaag gucgugggga ucgagaagga cggccgcauc     3480

aaguucaagu accaccagga ggcccggaag gaggggcugc ccaucuucag cacccccuac     3540

aagaacaacg acgacuacgc ccccaucuuc cgccagucca ucaacaacau caacauccuc     3600

guggacggca ucgacuucac gaucgacauc cuggggaagg ucacccugaa ggaguga        3657


<210>  1729
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1729
augaacaagc gcauccuggg ccucgacacc gggaccaaca gccugggcug ggccgugguc       60

gacugggacg agcacgccca guccuacgag uucaucaagu acggggacgu gaucuuccag      120

gagggcguga agaucgagaa ggggaucgag agcuccaagg cggccgagcg gagcggcuac      180

aaggccaucc gcaagcagua cuuccggcgc cggcugcgca agauccaggu ccucaaggug      240

cuggugaagu accaccugug ccccuaccuc uccgacgacg accugcggca guggcaccuc      300

cagaagcagu accccaagag cgacgagcug augcuguggc agcgcacguc cgacaagaag      360

ggcaagaacc ccuacuacga ccggcaccgc ugccugcacg agaagcucga ccugaccguc      420

gaggccgacc gguacacccu ggggcgcgcc cucuaccacc ugacccagcg gcgcggcuuc      480

cugagcaacc ggcucgacac guccgcggac aacaaggagg acgggguggu caagagcggc      540

aucucccagc ugagcaccga gauggaggag gccgggugcg aguaccuggg cgacuacuuc      600

uacaagcucu acgacgagca gggcaacaag gugcgcaucc ggcagcgcua caccgaccgg      660

aacaagcacu accagcacga guucgacgcc aucugcgaga agcaggagcu guccagcgag      720

cugaucgagg accuccagcg cgccaucuuc uuccagcugc cccugaaguc ccagcggcac      780

ggggugggcc gcugcaccuu cgagcgcggg aagccccggu gcgcggacag ccacccggac      840

uacgaggagu uccgcaugcu cugcuucgug aacaacaucc aggucaaggg cccccacgac      900

cuggagcugc ggccccucac guacgaggag cgcgagaaga ucgagccccu guucuuccgg      960

aaguccaagc cgaacuucga cuucgaggac aucgccaagg cccuggccgg caagaagaac     1020

uacgccugga uccacgacaa ggaggagcgc gcguacaagu ucaacuaccg gaugacccag     1080

ggggugcccg gcugccccac caccgcccag cucaagagca ucuucgggga cgacuggaag     1140

acgggcaucg ccgagaccua cacccugauc cagaagaaga acggguccaa gagccugcag     1200

gagauggagg acgacgugug gaacguccuc uacagcuucu ccagcgugga gaagcugaag     1260

gaguucgccc accacaagcu gcagcucgac gaggaguccg ccgagaaguu cgcgaagauc     1320

aagcugagcc acuccuucgc cgcccugagc cucaaggcca uccgcaaguu ccugcccuuc     1380

cugcggaagg gcauguacua cacccacgcc uccuucuucg cgaacauccc gacgaucgug     1440

ggcaaggaga ucuggaacaa cgagcagaac cgcaaguaca ucauggagaa cgucggggag     1500

cucguguuca acuaccagcc caagcaccgg gaggugcagg gcaccaucga gaugcugauc     1560

aaggacuucc uggccaacaa cuucgagcuc cccgccgggg ccaccgacaa gcuguaccac     1620

cccagcauga ucgagaccua cccgcacgcc cagcgcaacg aguucggcau ccugcagcug     1680

ggguccccgc ggacgaacgc gauccgcaac cccauggcca ugcggagccu ccacauccug     1740

cgccgggucg ugaaccagcu gcucaaggag uccaucaucg acgagaacac cgaggugcac     1800

gucgaguacg cccgcgagcu gaacgacgcc aacaagcggc gcgcgaucgc cggcuggcag     1860

aaggagcagg acaagcagca caagaaguac guggacgaga uccgcaagcu guacaaggag     1920

gagaccggca aggacaucga gcccacccag acggacgucc ucaaguucca gcugugggag     1980

gagcaggacc accacugccu guacaccggg gagcagaucg gcaucaccga cuucaucggg     2040

agcaacccga aguucgacau cgagcacacc aucccccagu ccgugggcgg ggacagcacg     2100

cagaugaacc ucacccugug cgacaaccgg uucaaccgcg agaucaagaa ggccaagcug     2160

cccgcccagc ucuccaacca cgaggagauc cuggcccgga ucgagcccug gaagaacaag     2220

uacgagcaga agaucaagga gcgcgacaag cagcggaccu ucgcgggcau ggacaaggcc     2280

gugaaggacc ugcgcaucca gaagcggcac aagcuccaga uggacaucga cuacuggcgc     2340

ggcaaguacg agcgguucac caugacggag gucccggagg gguucagccg ccggcagggc     2400

accgggaucg gccugaucuc ccgcuacgcc ggccuguacc ucaagagccu guuccaccag     2460

gccgacuccc ggaacaagag caacguguac guggucaagg ggguggccac cgcggaguuc     2520

cgcaagaugu ggggccugca gagcgaguac gagaagaagu gccgggacaa ccacucccac     2580

cacugcaugg acgccaucac caucgccugc aucgggaagc gcgaguacga ccucauggcc     2640

gaguacuacc ggauggagga gacguuccgc cagggccggg ggagcaagcc caaguucucc     2700

aagcccuggg ccaccuucac cgaggacgug cugaacaucu acaagaaccu gcucgucgug     2760

cacgacaccc ccaacaacau gccgaagcac accaagaagu acgugcagac gagcaagggc     2820

aagguccugg cgcagggcga caccgcccgc gggucccugc accucgacac cuacuacggc     2880

gccaucgagc gggacgggga gauccgcuac guggugcgcc ggccccugag cuccuucacc     2940

aagcccaagg agcuggagaa caucgucgac gagacgguga agcgcaccau caaggaggcc     3000

aucgcggaca agaacuucaa gcaggccauc gccgagccga ucuacaugaa cgaggacaag     3060

ggcauccuca ucaagaaggu gcggugcuuc gccaagagcg ucaagcagcc caucaacauc     3120

cgccagcacc gggaccuguc caagaaggag uacaagcagc aguaccacgu ggugaacgag     3180

aacaacuaca ugcuggucau cuacgagggg cucgugaaga acaagguggu ccgcgaguuc     3240

gagaucguga gcuacaucga ggccgcgaag uacuacaagc ggucccagga ccgcaacauc     3300

uucagcucca ucgugcccac ccacagcgcc aaguacggcc ugccccugaa gaccaagcuc     3360

cugaugggcc agcugguccu cauguucgag gagaacccgg acgagaucca gguggacaac     3420

acgaaggacc uggugaagcg gcuguacaag gucgugggga ucaagaagga cggccgcgug     3480

aaguucaagu uccaccagga ggcccggaag gacgggcucc ugaucuucuc cacccccuac     3540

aagaacaaca acggcuacgu ccccaucuuc cgccacagca agaagaacau caacauccug     3600

guggacggga aggacuucac caucgacauc cucggcaagg ugacccugaa ggaguga        3657


<210>  1730
<211>  3660
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1730
augaaggagg aggugcgcgg caagaaccgg agccagaccu gggccauccu ggccaaguuc       60

uucgucuggu ccgagaagga gagcgugugg aacgagaagg aggcgcgcac cgaggagcgg      120

gugaaggagu acaagcucgu cgggcugaag cgcgacacga agggcaacga gcugaagcuc      180

gagaacuacc gguggcgcgu gcaggcccug uccgagcagc ugaaccccga gaucguggcc      240

aucguccucc aggagaucaa cgggcagauc agcgccucca gcggcuaccu gggggccauc      300

uccgaccgga gcaagcagcu guacuucaac cgccagaccg ugggccagua ccucaugucc      360

gagcuggaga aggacccgaa cgugagccug cggaaccgcg ucuucuaccg gcaggacuac      420

cucgacgagu ucgaccgcau cugggagaag caggcggagu uccacgagga gcugaccgug      480

gagcugaaga aggagauccg ggacaucauc aucuucuacc agcgccggcu caagucccag      540

aagggccuga ucagcuucug cgaguucgag aagcgcgagg uggucgugga ggaggacggg      600

cggaagaaga acaagaccgu gggcugccgc gucauccccc ggucccaccc ccuguuccag      660

gaguucaaga ucuggcagac gcucaacgac aucgaggugu ucgccuggga caagcagagc      720

aagcgcaaga aggccgacaa guccagcauc cuguucgaca acuccgagga cgcccugcuc      780

guggagggga agcgguuccu guaccaggag gagaaggagc ugcucgcgaa ggagcuguuc      840

gucaaggaga gcaugaagaa ggccgaggug cugaagcucc uguucgagaa cuaccaggag      900

cuggaccuca acuucaagca gaucgacggc aaccacaccg gguucgcccu guucuccgcc      960

uacagcaaga ugaucgagaa guacggcuac gagcccgugg acuucaagcg cagcgccgac     1020

gagaucaucg agaagcugga gaccaucuuc accaaccucg gcuggaacac ggagcugcug     1080

uccaucgacc ucagcaagga ggacaaggag cuggagcgcc aguccuacuu ccggcugugg     1140

caccuccugu acagcuucga gggggacaac accccgaccg gcaacgggaa gcugcucgag     1200

aagaucaugc agcugugcga cgucgagaag gaguacgcgg uggagcuggc caacgugucc     1260

uuccaggagg acuacggcag ccucuccgcc aaggccauca agaagauccu gcccuaccug     1320

aaggagggca accaguacga cgucgccugc gacuacgcgg gguaccgcca cagccggucc     1380

agccucacca aggacgagau cgagaacaag auccugaagg acaagcugga gauccugccc     1440

aagaacuccc uccgcaaccc cguggucgag aagauccuga accagauggu gaaccugauc     1500

aacacgcuca ucgacaccua cggcaagccg gacgagaucc ggguggagcu ggcccgcgag     1560

cugaagaaga acgccaagga gcgggaggag cucaccaaga gcaucgcccg caacacccgg     1620

gagcacgacg agauccgcca gcugcugcgg acggaguucg ggaugaugaa cgucucccgc     1680

aacgacauca uccgguacaa gcucuacgag gagcugaagg acaacggcua caagacccug     1740

uacagcaacg aguacauccc ccgcgagaag aucuucucca aggagaucga caucgagcac     1800

gugauccccc aggcccgccu cuucgacgac agccugucca acaagacccu ggaguaccgg     1860

gcgaucaaca ucgagaaggg gaacaagacc gccuacgacu ucgugaagga gaaguacggc     1920

aacgacggcc ucgagaaguu ccugaaccgg ugcgagacgc uguucaagga caagcgcacc     1980

aagcuccgga agcugaagau ggaggagaag gacauccccg agggguucau cgaccgcgac     2040

cugcgcaaca cccaguacau cagcaagaag gccuucgcca ugcucaacga gaucucccgg     2100

cgcgucgugg ccaccacggg cagcaucacc gacaagcugc gggaggacug gcagcuggug     2160

gaccucauga aggagcugaa ccugccgaag uacgagaagc ucgggauggu cgagauguuc     2220

gaggacaagg acggccgcaa gaucaagaag aucaccgacu ggaccaagcg gaacgaccac     2280

cgccaccacg cgguggacgc ccugacggug gccuucacca aggacgucuu cauccaguuc     2340

uacaacaaca agaacgccgc guggaugccc gccagcaagg agcacgccaa caucaccggg     2400

aucaaggccc gguacuucga gaacggcaag gcccuggcgc ccaugccccu cgcccaguuc     2460

cgcgccgagg ccaagcggca ccuggagaac cugcucgugu ccaucaaggc caagaacaag     2520

gugaucacca gcaacgucaa ccgcacgaag aacaagaacg gcgagaaccg gaagcugcag     2580

cagaccccgc gcgggcagcu gcaccucgag accguguacg gcucccaccg gcaguacgcg     2640

accaagaucg agaaggugaa cgccgccuuc gacgucacga agaucgccac cgugagcaag     2700

cuggccuacc gcaacgcgcu gcucaagcgg cuggaggccu ucgggaacga ccccaagaag     2760

gccuucaccg gcaagaacac ccuggagaag aacccccucu accuggacga gaaccagacc     2820

gagauggugc ccgagaaggu ccagacggug gaguucgaga ccguguacac cauccgcaag     2880

ccgaucgacc ccaaccuguc caucgacaag gucguggacg ugaagauccg gagcguccuc     2940

gagaagcgcc ugaaggagua cgggggcgac gccaagaagg cguucgugaa ccuggacgag     3000

aaccccaucu ggcucaacga ggccaagggc aucuccauca agcgggugag cauccgcggg     3060

aucuccaacg cccagagccu gcacgagaag aaggacaagg acggcaaccu gauccucggg     3120

aaggagggcc ggaagauccc cgucgacuuc gugaacaccg gcaacaacca ccacguggcc     3180

aucuacaaga agccgguccu ggacaaggug ggccaggugg uccuggacga ggaggggaac     3240

cccaaguacg agcucgacga gguggugguc uccuucuucg acgccgugac gcgcgcgaac     3300

cuggggcagc ccaucaucga caaggacuac cgccggagcg agggcuggca guuccuguuc     3360

uccaugaagc agaacgagua cuucguguuc cccaacgaca agaccggguu caacccgaag     3420

gaggucgacc uccugaaccc cgagaacuac gcccugauca gccccaaccu cuuccgcgug     3480

cagaaguucg ccuacaagaa cuacguguuc cggcaccacc uggagaccac caucaaggac     3540

acguccagca cccugaaggg caucaccugg accgacuucc gcuccagcaa gggccucgac     3600

aagaucguca aggugcgggu ggaccacauc gggcagaucg ucagcaucgg cgaguacuga     3660


<210>  1731
<211>  3663
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1731
augaagcgca uccugggccu cgaccugggg agcgccucca ucggcugggc ccugaucgag       60

gaggacgaga aggggauccg gaucaucgcg cucggcagcc gcaucauccc cuacaacggg      120

accgagggcc aggacuucgc caagggcgcc ggggaguccc ggaacagccu gcgcacccag      180

gcccggacga cccgcaaggg cuacgaccgg uaccagcugc gccggaagua ccucaucaac      240

gugcuggaga agaaccugau gaugccgucc gaggaccucc gcaagaugcc caagaugaac      300

cuguggcagc ugcggagcaa ggccgucacc gaguacaucu cccgccagga gcucgggcgg      360

cugcugcucu ggcugaacca gaagcgcggc uacaagagcu cccggagcga cgcgaaccug      420

gacaagaagg acaccgacua cgugaaggag gugaaguccc gcuucgacaa gaucaaggag      480

aagaaccaga cgaucgggca guacuucuac gagagccucc agugcgacga guacuuccgg      540

gucaaggaga acguguuccc ccgcgaggcc uacaucgccg aguucgacgc caucugcucc      600

cggcagaagg accaccuggg ccugagcgag gagcucaucg cggagauccg caacgagauc      660

aucuacuacc agcggccccu gaagucccag aagggccugg ugagcgucua cgacuucgag      720

gggaagugga ugaaccagaa cggcaaggag cuguucaucg ggccgaaggu ggcccacaag      780

uccagccccc ucuuccagcu ggccaagauc ugggaguccg ugaacaacau caagcucagc      840

accaaguacg gcgacaccau cgagcugacc cuggaggaga agaagcagcu cgucgaguuc      900

cuggacaaca acgagaagcu gacgaccgcc gcccucuuca agauccugcg caaggacaag      960

aaggaguaca uccugcccaa gcagcucgag aagggccugc aggggaacau caccaagugc     1020

aagaucgcgg acauccuggg cagcaaguac aaccagcucc ugcagcugaa ccucgagaag     1080

caggaggugg ggacccccug cuaccuguac acgaagucca ccggcgaggu gcugggggag     1140

aagccgcucc aguacaucga caagagcguc gagaaggagc uguucuacca gcuguggcac     1200

accaucuacu ccaucagcga cgaggacgag ugcugcaagg cccuccagaa gcgcuucgac     1260

aucgacgccg agaccgccgg ccggcuggcc aagaucgacu ucuccaagua cggcuucggg     1320

aacaagagcg cgaaggccau ccgcaagauc cugcccuacc ugauggaggg cgacaaguac     1380

uccgacgcca ugugcuacgc cggguacaac cacagcaacu cccucacgaa ggaggagaac     1440

cacgagcgga agcugaacga caagcugaag cuccugacca agaacagccu gcgccagccc     1500

aucguggaga agaucaugaa ccagaugauc cagcucguga acaccaucau cgacaaguac     1560

ggcaagcccg acgagauccg gcuggagcug gcccgcgagc ucaagcaguc caaggaggag     1620

cggaacgacg cggacaaggc caugagcaag cgccagcggg agaacaagga ggccgagaag     1680

aagcuggagg agcacgggau ccgcgccacc cacaacaaca uccugaagua ccgccucuuc     1740

gaggagaugg gcgagaagaa gcacgaggcc aucugcaucu acugcggcaa cccgaucucc     1800

uucagcgcgg ccaugaacgg ggacgagguc gacguggagc acaucauccc ccgguccaag     1860

cuguucgacg acagccaguc caacaagacg cuggcccacc ggaagugcaa cgccaacaag     1920

aacgacauga ccgcguacga cuucaugaag accaagagca agcaggacuu cgacaaguac     1980

guggagcgcg ucaaccagcu cuacgccgac cggaucaucu cccgcagcaa guacaacaag     2040

cugcugauga gcgaggacga cauccccucc aacuucaucg accggcagcu ccgcgagagc     2100

caguacaucg ccaccaaggc ccgggagauc cugcagacga ucugcuacaa cguguggucc     2160

accagcggca ccgugaccgc cgagcugcgc caccucuggg ggugggacga caucacgaug     2220

aaccugcaga ugcccaagua caaggagcug ggccucaccg agauggucga guggaccucc     2280

gaccacggga agaacgugca cagcaaggag gugaucaagg gcuggaccaa gcgcgacgac     2340

caccggcacc acgcgaucga cgcccugacg aucgccugca ccaagcaggg cuucauccag     2400

cgcuucaaca cccuguccag cuccaagacc cgggacgaca ugcagaagga gcucgagaag     2460

ugcagcaucc aguuccgcga gaagcggucc cugcuggaga aguacaucau cagccagcgc     2520

ccgaucuccg ucagcgacgu ggccgaggcc gucucccgga uccucaucag cuucaaggcg     2580

gggaagaagg uggccacgau cggcaagcgc aaggugaagg ggaaggucgu gcagcagggc     2640

aucguggucc cccggggcgc ccuguccgag gggagcgugu acggcaagau ccgcaccauc     2700

gugcggaaga ugcccgucaa guaccuguuc gagaaccccg accucaucuu caagccguac     2760

aucaaggccc uggugaagga gcgccuggcc gaguucgaca acgacaacaa gcaggcgcuc     2820

gccagccuga agaagaaccc cguguaccug gacaaggaga agaccaagcu ccuggaguac     2880

gggaccugcu ucaaggacga guacgucaag aaguacgacg ugaaccccaa cuucaccaag     2940

aucgccgacg ugaucgacgg caaggucaag cagauccugg aggaccggcu cuccaaguuc     3000

gggggcaagc ccaaggaggc cuucaaggac guggugaagg acggcaagac gcugaagugg     3060

uacgaggacg aggggcugga gcgcccgauc cggagcgucc gcugccucac cggccugucc     3120

gcgguggugc ccgucaagaa ggacgagaac gggauggaca ucggcuucgu gaagcccggg     3180

aacaaccacc acguggccau cuacgucggc gccgacggca aggggcagga gcacgugugc     3240

accuucugga acgccgugga gcggaagaag uacggccugc ccaucaucau caaggacgcc     3300

aacgaggucu gggacaagau caccauggcg ggggacgacg uguacccgca gagcuuccuc     3360

ucccugcugc ccaagcccga cuggacgcuc aagcugagca ugcagcagaa cgagauguuc     3420

auccugggca uggaggagga cgaggugaag uucgccaucg aguccaagga cuacaagcuc     3480

cugagcgacc accuguaccg cguccagaag cucuccagcu ccgacuacaa cuucauccac     3540

caccuggaga ccgacgugca gaagaagacc augaccuacc gggugcgcag ccuguccggc     3600

cucuucgccc ugcggccguu caagguccgc aucgacugcc uggggaacau cauccccagc     3660

uga                                                                   3663


<210>  1732
<211>  3669
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1732
augaagaagc gcauccuggg ccucgacagc gggaccaacu cccugggcug ggccgugguc       60

gaccgggacg agaacaacca guacagccug aucaaguacg gggaccucau cuucgaggag      120

ggcgugaaga ucgagaaggg gguggagucc agcaaggccg cggagcgcac cucccaccgg      180

gcccugcgca agcuguacuu ccggcgccgg cuccgcaaga ucgagguccu gaaggugcug      240

gugaaguaca accucugccc ccaggucagc gaggaggagc ugcaccugug gcacacgcgg      300

aagcucuacc cgcagaacaa ggaccugcug caguggcagc gcaccgacga gaaggccggc      360

aagaaccccu acaucuaccg guaccgcugc cuccacgagg agcuggacau guccgugagc      420

gccaaccgga ugaccguggg ccgcgccaug uaccaccugg cgcagcggcg cggguuccuc      480

uccaaccggc uggacaacga ggaggacgag aaccagaucg gcgccgucaa gugcgggauc      540

agcaaccuga ccgaggagau ccaguccagc ggcugcaagu accucgggga guacuucuac      600

aagcuguaca acgagcacgg caacacggug cgccugcgga cccgcuacac cucccgggag      660

uuccacuaca agaaggaguu cuacgccauc ugcgagaagc agaagcucga caagaagcug      720

aucgaggagc uggccagcgc gcucuacuuc cagcgccccc ugaaguccca gcgccacacc      780

gugggccggu gcacguucga gcccaacaag ccgcgcugcg ccgacagcca ccccgccuac      840

gaggaguucc ggaugcuguc cuucaucaac accaucaagg uccaggggcc cuacgacacc      900

gagcuccgcg ugcugaacac cgaggagcgg gacagcauca cgaagcuguu cugccgcaag      960

uccaagccca acuucgacuu cgaggacauc gccaaggagc ucgccggccg gaacaacuac     1020

ugcuacaucg gggacguggu cgacaaggcg uacaaguuca acuaccgcau gagccagggc     1080

gugagcggcu gcccgaccac caccuaccug aucaacgugu ucgggcagga cuggcagacg     1140

ggcaucgccg aguccuacca ggccaucgac accaagcugg ggcagaaguc cgucgacgcc     1200

augaucgacg acguguggaa cgugcucuac uccuucagcu ccaaggacaa gcugcgggac     1260

uucggccgca agaagcugca gcucggggac aaggaggccg acaccuucgc gaacaucaag     1320

cugacccaca aguucgccag ccugucccuc aaggccaucc ggaagaucaa caucuuccug     1380

cgcaugggcc uggucuacag ccacgccgug acgcucgcca acauccccac caucgugggc     1440

accgacaucu ggcagcggaa gcgcaacuuc auccuggcgg agcugcuccc ccugaucgag     1500

aacuucaacc ccaaggagac caagcugcag gagacgcugg acuucugcgu caaggacuuc     1560

cuccagaaca acuucgagcu gaagaacggg gccguggaca agcuguacca cccguccaug     1620

aucgaggccu accccgacgc cggccucaac agcaacggga uccugcagcu gggcagcccc     1680

cggaccaacg ccauccgcaa ccccauggcg augcggagcc uccacgagau ccgcaagguc     1740

aucaaccagc ugcugaagga caagaucauc aaccaggaca ccgagaucca cguggaguac     1800

gcccgggagc ucaacgacgc caacaagcgc aaggccaucu ccaucuggca gaagucccag     1860

gagaagcugc acaagaagua cguggaggag aucaccaacc uguacaagga ggagacgggg     1920

aaggcgaucg ucccgaccga caccgacauc cucaaguuca agcuguggga ggagcagaac     1980

cacaucugcc uguacaccgg ccagcagauc ggcaucgccg aguuccucgg ggccacgccc     2040

aaguacgaca ucgagcacac caucccccag agcgugggcg gggacagcac cgacaugaac     2100

cugacccugu gcgacucccg cuucaaccgg gagaucaaga aggcccagcu ccccacgcag     2160

cugggcaacc acgccgacau ccuggugcgc aucgcggggu ggaagaacag cuacgagaag     2220

cucuccaagg acaucgaccg gauccgcacc cacagcggca uggccaagga caucaaggac     2280

cggcugaucc agaagcgcca ccuccugaag cuggagcggg acuacuggcg cggcaaguac     2340

gagcgguuca ccaugaacga gaucccggag ggguucagcc gccggcaggg cgucgggauc     2400

ggccugaucu ccaaguacgc cggccucuac cucaagagcc uguuccacga caagaacgag     2460

cgcaacaagg ggcacguguu cguggucaag ggcaccacca cggccgaguu ccggaagaug     2520

ugggggcugc agaacgagua cgagaagaag ucccgcaaca accacgugca ccacugcauc     2580

gacgccauca ccaucgcgug caucggcaag cacgaguacg cccagauggc ccaguacuac     2640

cacgacgagg ccgaguaccu guaccaccac gggaucaagc ccaucuuccc caagcccugg     2700

aagaccuuca ccgaggaccu gcucgaccug gagcacagcc uguucguggu ccaccacucc     2760

ccgaacaaca uggccaagca ggcgcggaag cgcaucauca cgagcaccgg ccagcacugg     2820

gcccagggcg acuccgccca cgggagccuc cacaacgaca ccuacuacgg cgccaucgag     2880

cgggacgggg agaucaagua cguggugcgc cgggcggucu ccagcuucga gcagaugucc     2940

gacguggaca gcaucgugga cgacgucgug aaggagaccg ugcgcaaggc ccuggagggc     3000

aagaacuuca aggaggccgu caacgccccc aucuacauga acaaggagaa gggggugcug     3060

aucaagaagg ugcggugcua cgccaagucc aucaagaagc ccaucgacau ccgcacgcac     3120

cgcgacagcu ccagcaagga guacaagcgg aaguuccacg ucuccaacga caucaacuac     3180

cucaucgcga ucuacgaggg caccgugaag ggcaagguga agcgcgacuu cgaggucgug     3240

aagaacaucg aggccgccaa cuucuucaag cggagccagg accgccggga cuaccccacc     3300

aucgugccgg agaaguccau caagcacggg uucccccuga agaccacgcu gaccaacggc     3360

accaugguca uccucuacga ggagaacaag gaggagaucg acuucaccaa gaaggaggac     3420

aucugccgcc ggcuguacaa gcuggugggg augagcagca ugaucguguc cggcugcgag     3480

uacggggucc ucagccugcg ccaccaccag gaggcccggc ccuccaagga caucaagagc     3540

aagaacggcg ccuucaagaa cggcgaggcg cugcgcccgg ccaucacgau gcuccacacc     3600

caguucaacg cccuggugga gaacaucgac uucaacaucg acgugaccgg gaacaucgag     3660

cugaucuga                                                             3669


<210>  1733
<211>  3669
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1733
augaagaagc gcauccuggg ccucgacagc gggaccaacu cccugggcug ggccgugguc       60

gaccgggacg agaacaacca guacagccug aucaaguacg gggaccucau cuucgaggag      120

ggcgugaaga ucgagaaggg gguggagucc agcaaggccg cggagcgcac cucccaccgg      180

gcccugcgca agcuguacuu ccggcgccgg cuccgcaaga ucgagguccu gaaggugcug      240

gugaaguaca accucugccc ccaggucagc gaggaggagc ugcaccugug gcacacgcgg      300

aagcucuacc cgcagaacaa ggaccugcug caguggcagc gcaccgacga gaaggagggc      360

aagaaccccu acaucuaccg guaccgcugc cuccacgagg agcuggacau guccgugagc      420

gccaaccgga ugaccguggg ccgcgccaug uaccaccugg cccagcggcg cggguuccuc      480

uccaaccggc uggacaacga ggaggacgag aaccagaucg gcgcggucaa gugcgggauc      540

agcaaccuga ccgaggagau ccaguccagc ggcugcaagu accucgggga guacuucuac      600

aagcuguaca acgagcacgg caacacggug cgccugcgga cccgcuacac cucccgggag      660

uuccacuaca agaaggaguu cuacgccauc ugcgagaagc agaagcucga caagaagcug      720

aucgaggagc uggccagcgc ccucuacuuc cagcgccccc ugaaguccca gcgccacacc      780

gugggccggu gcacguucga gcccaacaag ccgcgcugcg cggacagcca ccccgccuac      840

gaggaguucc ggaugcuguc cuucaucaac accaucaagg uccaggggcc cuacgacacc      900

gagcuccgcg ugcugaacac cgaggagcgg gacagcauca cgaagcuguu cugccgcaag      960

uccaagccca acuucgacuu cgaggacauc gccaaggagc ucgccggccg gaacaacuac     1020

ugcuacaucg gggacguggu cgacaaggcc uacaaguuca acuaccgcau gagccagggc     1080

gugagcggcu gcccgaccac caccuaccug aucaacgugu ucgggcagga cuggcagacg     1140

ggcaucgcgg aguccuacca ggccaucgac accaagcugg ggcagaaguc cgucgacgcc     1200

augaucgacg acguguggaa cgugcucuac uccuucagcu ccaaggacaa gcugcgggac     1260

uucggccgca agaagcugca gcucggggac aaggaggccg acaccuucgc caacaucaag     1320

cugacccaca aguucgcgag ccugucccuc aaggccaucc ggaagaucaa caucuuccug     1380

cgcaugggcc uggucuacag ccacgccgug acgcucgcca acauccccac caucgugggc     1440

accgacaucu ggcagcggaa gcgcaacuuc auccuggccg agcugcuccc ccugaucgag     1500

aacuucaacc ccaaggagac caagcugcag gagacgcugg acuucugcgu caaggacuuc     1560

cuccagaaca acuucgagcu gaagaacggg gcgguggaca agcuguacca cccguccaug     1620

aucgaggccu accccgacgc cggccucaac agcaacggga uccugcagcu gggcagcccc     1680

cggaccaacg ccauccgcaa ccccauggcc augcggagcc uccacgagau ccgcaagguc     1740

aucaaccagc ugcugaagga caagaucauc aaccaggaca ccgagaucca cguggaguac     1800

gcgcgggagc ucaacgacgc caacaagcgc aaggccaucu ccaucuggca gaagucccag     1860

gagaagcugc acaagaagua cguggaggag aucaccaacc uguacaagga ggagacgggg     1920

aaggccaucg ucccgaccga caccgacauc cucaaguuca agcuguggga ggagcagaac     1980

cacaucugcc uguacaccgg ccagcagauc ggcaucgcgg aguuccucgg ggccacgccc     2040

aaguacgaca ucgagcacac caucccccag agcgugggcg gggacagcac cgacaugaac     2100

cugacccugu gcgacucccg cuucaaccgg gagaucaaga aggcccagcu ccccacgcag     2160

cugggcaacc acgccgacau ccuggugcgc aucgccgggu ggaagaacag cuacgagaag     2220

cucuccaagg acaucgaccg gauccgcacc cacagcggca uggcgaagga caucaaggac     2280

cggcugaucc agaagcgcca ccuccugaag cuggagcggg acuacuggcg cggcaaguac     2340

gagcgguuca ccaugaacga gaucccggag ggguucagcc gccggcaggg cgucgggauc     2400

ggccugaucu ccaaguacgc cggccucuac cucaagagcc uguuccacga caagaacgag     2460

cgcaacaagg ggcacguguu cguggucaag ggcaccacca cggccgaguu ccggaagaug     2520

ugggggcugc agaacgagua cgagaagaag ucccgcaaca accacgugca ccacugcauc     2580

gacgccauca ccaucgccug caucggcaag cacgaguacg cgcagauggc ccaguacuac     2640

cacgacgagg ccgaguaccu guaccaccac gggaucaagc ccaucuuccc caagcccugg     2700

aagaccuuca ccgaggaccu gcucgaccug gagcacagcc uguucguggu ccaccacucc     2760

ccgaacaaca uggccaagca ggcccggaag cgcaucauca cgagcaccgg ccagcacugg     2820

gcgcagggcg acuccgccca cgggagccuc cacaacgaca ccuacuacgg cgccaucgag     2880

cgggacgggg agaucaagua cguggugcgc cgggccgucu ccagcuucga gcagaugucc     2940

gacguggaca gcaucgugga cgacgucgug aaggagaccg ugcgcaaggc gcuggagggc     3000

aagaacuuca aggaggccgu caacgccccc aucuacauga acaaggagaa gggggugcug     3060

aucaagaagg ugcggugcua cgccaagucc aucaagaagc ccaucgacau ccgcacgcac     3120

cgcgacagcu ccagcaagga guacaagcgg aaguuccacg ucuccaacga caucaacuac     3180

cucaucgcca ucuacgaggg caccgugaag ggcaagguga agcgcgacuu cgaggucgug     3240

aagaacaucg aggcggccaa cuucuucaag cggagccagg accgccggga cuaccccacc     3300

aucgugccgg agaaguccau caagcacggg uucccccuga agaccacgcu gaccaacggc     3360

accaugguca uccucuacga ggagaacaag gaggagaucg acuucaccaa gaaggaggac     3420

aucugccgcc ggcuguacaa gcuggugggg augagcagca ugaucguguc cggcugcgag     3480

uacggggucc ucagccugcg ccaccaccag gaggcccggc ccuccaagga caucaagagc     3540

aagaacggcg ccuucaagaa cggcgaggcc cugcgcccgg cgaucacgau gcuccacacc     3600

caguucaacg cccuggugga gaacaucgac uucaacaucg acgugaccgg gaacaucgag     3660

cugaucuga                                                             3669


<210>  1734
<211>  3678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1734
augagcaagc gcauccuggg caucgacgug gggaccaacu cccucggcug ggccgucgug       60

gaccggaccg acugcgacaa cuacacgcug gugaagcgcg gggacgucau cuuccaggag      120

ggcgugaaca ucgagaaggg gaacaucgag aagucccggg ccagcgagcg caccggccac      180

cggagcaccc gcaagcacua cuuccggcgc cggaugcuga agaucaaggu gcucgagauc      240

cuggucgagc accagcugug ccccuaccuc ucccacgagg agcugcacca guggaugacc      300

cgcaaggugu acccgacgaa cgaggacuuc cggcuguggc uccgcaccaa cgacgcgaac      360

gaccagaacc ccuaccacga ccggcacgug ugccugcacg agcgccugga ccuccggcug      420

caggagaacc gcuaccggcu gggccgcgcc cucuaccacc uggcccagcg gcgcggguuc      480

cugagcaacc ggcucgagca gaacuccaag accgacgaga ccggcgccgu caagagcgac      540

aucucccagc ugaacgagga gaugaagaag gccgggagcc aguaccuggg cgacuacuuc      600

uacuggcucu accaggagua cgggaaccag gugacgcugc gcauccgcua caccgaccgg      660

aagcagcacu accagaagga guucgaggcg aucugccagc gccagcagcu guccgacgac      720

cucguggaca agcugcggcg cgccaucuac uuccagcgga gccugaaguc ccagcgcgcc      780

ggcgucggca agugcaccuu cgagcccgac aagccccggu gcgcccucag ccacccgcgc      840

uacgaggagu uccggaugcu guccuuccug aacaacguga aggugaaggg gcccagcgac      900

gacucccucc gcccccugag ccccuccgag aaggagaaga ucaagccgcu guuccuccgc      960

aagagcaagg cgaacuucga cuucgaggac aucgccaagg ccaucgccgg caaggggaag     1020

uacgccugca uccgggacaa ggagggcgcg cagaagcccu accaguucaa cuacgcccug     1080

agccagggcg ucuccgggug ccccgugacc gccaagcuga ucgccguguu cggcgagagc     1140

ugggagcgga accucgucaa cgccuaccgc ucccggaacg acaugcgcaa gagcgacgag     1200

gagaucguga acgaggugug gaacguccug uacuccuuca gcuccaagga gaagcugcgg     1260

gcguggggga uggagaagcu cggccuggac gaggccugcg ccggggaguu cgccgacauc     1320

acgcugccca ccgccuucgc ggcccucagc cugaaggccg ugaacaagau ccugccguuc     1380

cuccgccagg gcuucaucua cucccacgcc gugaugcugg ccaacauccc cuacaucauc     1440

ggcauggacc gguggaacga gccccgccag cgccaguuca ucauggacaa ccugcaggag     1500

cuccuggaca acuucaccgc gagcgacaag gcccugcagc agacccugga cuucugcauc     1560

aaggacuucc uccugaacaa cuacgagcug gcccccggga aggccgaccg gcucuaccac     1620

ccguccauga ucgagacgua ccccgacgcg aaggccgacg gccaggggau cuaccagcug     1680

ggcagccccg ccaccaacgc cauccgcaac cccauggcca ugcggucccu gcacauccuc     1740

cgccgggucg ugaaccagcu gcugaaggag aagaccaucg acagcgagac cgagguccac     1800

aucgaguacg cgcgcgagcu caacgacgcc aaccggcgcu gggccaucaa ccaguggcag     1860

cggaagcagg agacgcagcg caaggacuac gccgagaaga uccggaaggu guacgccgac     1920

gaguucgaga aggugaucga gccgacccag gaggagcugc ugaaguacca gcucugggag     1980

gagcagcagc accugugccu guacaccggg gagcagaucg gccucaccga cuucaucggc     2040

cccaaccccc gcuacgacau cgagcacacg aucccccagu ccaugggcgg ggacagcacc     2100

cggaugaacc ugacccugug cgagcgcacc uacaaccgga cggucaaggg gaaccgcaug     2160

ccgucccagc ucgcggagca cgagagcauc cugacccgga ucaagcccug gaaggacaag     2220

uacgaggagc ugaccgugaa gaucaacaag cuccgcaccc accccggcau ggacaaggcc     2280

uccaaggaca acaucauccg gaagcgccac cuccuggagc ucgagcggga cuacugguuc     2340

cagaaguacc agcgcuucac gaugaccgag gugcccgagg gguucagccu gcgccagggc     2400

accggcaucg ggcugaucgg caaguacgcc gggcuguucc ucaagagccu guuccaccac     2460

gucaaggagc ggaaccgcaa gcaggucuac gugaucaagg gcccgaucac cgccgaguuc     2520

cggcgccugu ggggccugca gaaccuguac gagcagaagu cccgggacaa ccacgcccac     2580

cacugcaucg acgcgaucgu gaucgccugc aucaaccccg ccgaguacgc ccgcauggcg     2640

agcuacuacc ggcaggagga ggacuuccgc cucaacaagg ggaacaagcc cguguucccg     2700

aagcccugga cgaccuucac ccaggaccug caggcccugg aggccgagcu ccuggugguc     2760

cacgagaccc ggaacaaccu gaccaagaag gcccgcaagc gggugcgcac gaagggcggg     2820

aacgugcucu ccgagagcga cuccgcccgg ggcagccugc acaacgacac cuacuacggg     2880

gcgauccaga aggacggcgu cauccgcuac guggugcgga aggcccuguc cagccucucc     2940

gagaaggacg ucaaggacau cguggacaag accgugcgcg agaaggucca ggccgccauc     3000

caggagaagg gcgccaagga ggcgcuggcc gggaccaucu acaugaacga ggagaagggc     3060

aucacgauca agaaggugcg gugcuucacc aagaucgcca gcccccuggc cauccgccgg     3120

caccgcgacg ccuccgcgaa ggaguacaag cgccccuacu acgugaccaa cgagagcaac     3180

uacgucaugg ggaucuacga gggcugcauc aaggagaaga ccaagcggga guucauccuc     3240

gugaacaacc uggaggccgu gcgccagcug aaggccgccc ggaagcaggg ggucgugucc     3300

agcauccucc cgcccguguc cgccaagagc caguuccccc ugaaguaccg ccuguacacg     3360

ggcacccucg uccugcugua cgagaacucc cccgaggaga ucgacuucac caaccaggug     3420

gacaucugca agcggcucua caaggugacc ggccugagca gcaugacggu caccgggaac     3480

caguacggca ccauccagcu gcgcuaccac caggaggcgc ggcaggccaa ggaccucaag     3540

gcccagaacg gggccuacaa ggagggcgag gagcaccgcc cggcgaucau gaugcugcac     3600

acccaguuca aggcccuggu ggaggggacg gacuuccgga ugaacauccu cggcgagauc     3660

gagccccugc gcaaguga                                                   3678


<210>  1735
<211>  3690
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1735
augggcgacc gcaaguacaa ccuggggcuc gacaucggca ccagcuccau cggguucgcc       60

gccguggacg agaacaacca gcccauccgg gucaagggca agaccgcgau cggggugcgc      120

cuguucgagg agggcaagac ggccgccgac cggcgcggcu uccggaccac ccgccggcgc      180

cugagccggc gccgguggcg caucaaccuc cugaacgaga ucuucgacgc ccaccuggcc      240

gagguggacc cgaccuuccu cgcgcggcug aaggagucca accgcagcaa ccuggacccc      300

aagaaguccu uccaggggag ccuccuguuc cccgagcgga aggacuacca guucuacgag      360

gaguacccca cgaucuacca ccuccgcaag gcccugaugg agaaggaccg gaaguucgac      420

auccgcgaga ucuaccuggc cguccaccac aucaucaagu accggggcaa cuuccugaac      480

gggaccccga ugcgcuccuu caagguggag aacaucgagc ucaacacccu guucgaccag      540

cugaaccagc ucuacgccga gaucgugccc gacaacgagc uggcguucga ccuggcccag      600

gucgccgacg ugaaggacgu gcucagcucc accacgaucu acaagaugga caagaagaag      660

cagcugguca agaugaugcu gcuccccgcc agcaacaagg cccugcaguc cgagaacaag      720

aagaucguga cccaguucgu gaacgcgauc cugaacuaca aguucaagcu cgacguccug      780

cuccaggugg agaccgacgc cgacuggagc cugaagcuga acgacgaggg cgccgacgac      840

aagcuggagg aguucaccgg ggaccucgac gagaaccggc uggagaucau cgaccugcuc      900

cagcgccugc acaacugguu cucccugaac gagaucacga aggacggcaa cagccucucc      960

gccgccaugg uggagaagua cgagaaccac caccaccacc ugggccugcu caagaagguc     1020

aucgagaacc accccgacgc gaagaaggcc aaggcccuga aggagaccua caccgccuac     1080

guggggaaga ccgacgacaa gacgcagaac caggacgacu ucuacaaggc cguggagaag     1140

aaccuggacg acagcccgga cgcgaagggc aucaagcgcc ucauccagcu ggaccaguuc     1200

augcccaagc agcggaccgg gcagaacggc gccauccccc accagcugca ccagcaggag     1260

cucgaccaga ucaucgagaa gcaguccaag uacuaccccu uccuggccga gccgaacccc     1320

aacgucaagc gccggaagga cgcccccuac aagcuggacg agcugaucgc cuucaagauc     1380

cccuacuacg ugggcccgcu cgugaccccc gaggagcagg cgcagaacaa ggagaacguc     1440

uucgccugga ugaagcgcaa ggccgccggg cccaucaccc ccuggaacuu cgacgagaag     1500

guggaccgga uggagagcgc gaaccgcuuc auccggcgca ugacgaccaa ggacaccuac     1560

cuguucggcg aggacgugcu gccggccgag uccaugaucu accagaaguu cgucgugcuc     1620

aacgagcuga acaaccugaa gaucaacggg cggcaccuca gccugaagga caagcaggac     1680

gucuacaacg accuguucaa gcagcagaag accgugagca ucaaggcccu ccagaacuac     1740

uacgugacga agaagaaggc cgccaccgcg cccaccgucg gcgggcuggc cgaccccaag     1800

aaguuccugu ccagccucuc caccuacauc gacuucaaga acauguucgg cgagcgcgug     1860

aacgaccccc aguuccagga ggaccuggag cagaucgugg aguggagcac gaucuucgag     1920

gaccggggca ucuucaaggc caagcugcag gcccucgggu ggcuguccga gaagcagauc     1980

cagcagcugg ucgccaagcg cuacaagggc ugggggcggc ucagcaagaa gcugcugacc     2040

ggccucaaga acgcggaggg guacuccauc cuggacgaga uguggcgcag caccgagaac     2100

uucaugcaga uccagucccg gccggaguuc gccgcccuga uccagcaggc caacgagaag     2160

caguucgagg gcaacgaccc cgacaacgug ugggagaaca ucgagaacau ccucggcgac     2220

gccuacacca gcccccagaa caagaaggcg auccgccagg uggucaaggu ggugcaggac     2280

aucgagaagg ccgucgggaa cccgcccgag aagaucgcca ucgaguucac gcgggaggcc     2340

gcggccaacc cccagcgcac ccagucccgg cugcgcaccc uggagaagcu cuacgagagc     2400

gccgaggagg ugguggacgc cggccugacc gccgagcugg cggaguucaa ggagaacaag     2460

cacguccucu ccgacaagua cuaccuguac uucacgcagc uggggcgcga cguguacacc     2520

ggcgacacca ucagccucga caagcugaac gacuacgacg uggaccacau ccugccccag     2580

uccuucauca aggacgacag ccucgacaac cggguccuga ccauccgcgc cgugaacaac     2640

gggaaguccg acaacgugcc ggccaagaug uucggcaaga agaugggcag cuucuggcgg     2700

caccuccugg acaacgggau gaucuccaag cgcaaguaca acaaccugau caccgacccc     2760

gacaacauca gcaaguacgc ccagaagggc uucaucaacc ggcagcuggu cgagacgagc     2820

caggugauca agcucaccgc caacauccug aacgggaucu acgacaagga caccgagauc     2880

aucgaggugc ccgcgaagau gaacucccag augcgcaaga uguucgaccu ggucaaggug     2940

cgggagguga acgacuacca ccacgccuuc gacgccuacc ucaccaucuu caucggcaac     3000

uaccuguaca agugcuaccc caagcugcgc ccguacuucg ucuacgacaa cuucaagaag     3060

uucggcaaca aggaggacau cgggcacaag cgguucaacu uccucggcaa gaucgagcgc     3120

gagaagaagg ugguggcccc cgagacgggg gagauccugu ggagcaacgu cgcccccaac     3180

gagaccauca agcagaucaa gaagguguac aacuacaagu ucaugaucgu gucccgggag     3240

aucaccaccc gccgggcgga gcuguucaac cagacggucu accccaagaa cuaccgcggc     3300

aagcucaucc cgaucaagga ggaccggccc accgaccugu acgggggcua cagcggcaac     3360

accgacgccu accuggccau cguggcccuc gaggacaaga agaaggggaa guacuucaag     3420

guggucggca uccccacccg cgcggccgcc aagcuggaga agcugaagca gcaggacucc     3480

cagcaguacc uccaggcccu gcacgaggug aucgcgcccc aguucaccaa gagcacgaag     3540

aaggggauca agaagaccga guucgagauc gugcuggaca agguccacua ccggcagcuc     3600

gugcaggacg gcccggugaa gaugaugcug ggguccagca ccuacaagua caacgccaag     3660

cagcuggucc ucuccgagaa ggcccuguga                                      3690


<210>  1736
<211>  3696
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1736
augaucaccc gcauccuggg ccucgacacc gggacgaaca gccuggccug ggccgugguc       60

gaccgggacg cgaacggcaa cuacacccug gugaagcgcg gggacgugau cuucaccgag      120

ggcgucaaga ucgagaaggg gaucgagucc agcaaggccg ccgagcggac cgcccacaag      180

gcccuccgcg ugcacuacuc ccggcgccgg cugcacaaga ucgacacgcu gaaggugcuc      240

gucgagcugg accugugccc cuaccucagc aacgagcagc ugaaccugug gaaggcgaag      300

aagaccuacc cgcucaccga cgacuucaug cuguggcagc gcuccggcag cgccgaggag      360

aacaaccccu acuacugccg gcaccgcugc cugacccaga agcucgaccu gacggaccag      420

ucccagcggu uccugcucgg ccgcgccuuc uaccaccugg cccagcggcg cggguuccug      480

agcaaccggc ucgacaccuc cgcggacagc gacgaguccg gcaaggucaa gagcgggauc      540

gccgagcugu ccguggagau ggagaaggcc ggcugcgagu uccuggggga guacuucuac      600

cagcucuaca agcagcacgg caacaccauc aagcugcgca gccgguacac cgaccgcgag      660

gagcacuaca agaaggaguu ccuggccauc ugcgagaagc aggagcucga ccccaagugg      720

guggaggccc ugcagcgggc gaucuacuuc cagcgccccc ugaaguccca gcggaagggc      780

guggggaagu gcacguucga gccgaagaag ccccgcugcg ccgagagcca ccccgacuac      840

gaggccuucc gcaugcucug cuuccugaac aacgugaaga uccagacccc caacgacacc      900

ucccugcggc cgcucaacga ggacgagcgc gagaagaucu ggccccuguu cuuccgcaag      960

agcaagccca acuucgacuu cgaggacauc gccaaggccc uggcgggcaa gaacaacuac     1020

gccugguaca aggacgccgg ggagaagccc uucaaguuca acuaccggau gcagcagggc     1080

gucuccggcu gcccggugac cgcccagcuc aucgacaucu ucgggagcga cuggcagacg     1140

gccaucgcgg agaccuacac cgccguggag aagaagaacg gcaccaagac gaucgacgag     1200

auggccgacg acgucuggaa cgugcuguac agcuucucca gcaaggacaa gcugaaggcc     1260

uucgccaccg agaagcucca gcuggacgag gcguccgcca ccaaguucgc caacgccaag     1320

cugccccacg gguucgcgag ccucucccug aaggccaucc ggaacauccu gcccuuccuc     1380

cgccagggcu ucaucuacgc ccacgccgug caccuggcca agauccccga caucgucacc     1440

aaggcggugu ggaacgacgc cgacaagcgg gagaugaucc ugagcgggcu cauggagauc     1500

aucgagaacu acaacccgaa ggaccgcggc guggagggca cgaucgacuu cugcaucaag     1560

ucccaccucc aggacgucau cgaccugcgg cccggggccg ccgacgcccu guaccacccc     1620

agcaugaucg agaccuaccc cgacgcgaag cugaacaaga ugggcguguu ccagcugggg     1680

uccccgcgca ccaacgccau ccggaacccc auggccaugc gcagccugca cauccuccgg     1740

uccgugguca accagcugcu gcgcgacggc aucaucgacc ggaacaccga ggugcacguc     1800

gaguacgccc gcgagcucaa cgacgccaac aagcggaagg cgaucgccga cuaccagcgc     1860

gagcaggaga agaagcggaa gcaguaccgc gacgacauca ucgcccugua caagagcgag     1920

ugccacaagg agaucgagcc cacggacacc gacauccuga aguuccagcu cugggaggag     1980

caggaccacc ugugccugua caccgggacc cagaucggca ucugcgacuu ccucggcgac     2040

aaccccaagu ucgacaucga gcacacggug ccgcgcuccg ugggcgggga cagcacccag     2100

augaaccuga cccugugcga cgccaccuuc aaccggcagg ucaagggggc gcagcucccc     2160

ucccagcuga gcaaccacgc cgacauccug gugcgcaucg accacuggaa guccaagugc     2220

gaggaccucc ggaagcagcu ggacaagauc cgcacguacg gcgggaugga gaaggccaug     2280

aaggaccggc ugauccagaa gcgccaccgg cucaagaugg accuggacua cuggcagggc     2340

aaguacagcc gcuucaccau gaccgaggug cccgagggcu ucucccggcg ccagggggcc     2400

ggcaucgggc uggucagcaa guacgccggc cucuuccuca agagccuguu ccacaacgag     2460

cagaacccca acaaguccaa cguguacgug gucaagggcg ugaccaccgc ggaguuccgg     2520

cgcauguggg ggcugcaggg cgaguacgag aagaaguccc ggcugaacca cugccaccac     2580

ugcaucgacg ccaucacgau cgccugcauc gggaaggccg aguacgacaa gauggcccag     2640

uacuaccacg acgaggagcu guuccagugg cgcggcacca ccaccaagcc gcacuucaac     2700

aagcccuggc ccacguucac cgaggacgug cucgcgaucg agcagagccu gcuggucaag     2760

caccucacca aggacaacau gccgaagcag gccaagaagc gggugcgcac cgccacgggg     2820

aaguaccugg cccagggcga caccgcccgg ggcagccugc acaaggacac cuacuacggg     2880

gcgaucgagc aggacggcga gauccgcuac gugcuccgga agccccugaa caaguucgag     2940

aacgaggccg agcuggagaa gaucgucgac caggccguga aggagaagau caaggaggcc     3000

aucgcgggga agaacuucaa gaccgccauc acggagccca ucuucaugaa caaggagaag     3060

ggcauccgca ucaacaaggu gcgguacuac gucaaggccc aggagcgccu caagauccgc     3120

cagcagcggg accuguccag caaggaguac aagcaguucu ucugcgugga gaacgagucc     3180

aacuaccugc ucgccaucua cgagggggag cugaagggca agaaggagcg cgacuucugc     3240

guggucaaca accuggagag cgccgcgugc cucaagcggu ccaacaaggg caaggccgcg     3300

ccccuggugc cggagcgcag cgaggagcgg aaccugcccc uccuggccgu gcugaagcgc     3360

gggacccagg uccucuucua cgagaacucc cccgaggaga uccuguucga caacccccag     3420

gaccuggugc ggcgccucua caaggugacc ggcaucaaca ccaagaagga cgucaagaac     3480

gggaagacgu accuguacgg caacaucgag cugcgguucc accagaacgc ccagaagaag     3540

gacgacguga agaugcagag cgggguguac aagaacaacg agguccuccg ccagggcauc     3600

gugcuguccc acaagcaguu caaggcccug guggagggcg ucgacuucga guucaacgug     3660

cucggggaga ucaagaagaa ggagcggcac gccuga                               3696


<210>  1737
<211>  3699
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1737
augaccaaga cccggauccu gggccucgac acggggacca acagccuggg cugggccauc       60

guggaccggg acgaggucuc cgggcaguac gagcuggugg accgcggcga ccucaucuuc      120

accgaggggg ugaagaucga gaagggcauc gagagcucca aggccagcga gcggacccag      180

caccgcgcgc ugcggaagca guacuuccgc cggcgccugc ggaagaucga gguccuccgc      240

gugcugacgu ucuaccaccu gugcccgccc guguccgagg aggagcucca ccuguggcac      300

gucaagaaga ucuaccccgu ggagaaccac gaguucauga ucuggcagcg gaccaacgac      360

aacgagggca agaaccccua cuacuaccgc cacaucugcc ugcacaccaa gcucaaccug      420

acccugauga aggaccggua cauccucggg cgcgcccugu accaccugag ccagcggcgc      480

ggcuuccucu ccaaccggcu ggaccagagc caggacgagg acgacaaggg gacggugaag      540

accggcaucu ccagccuguc caaggacaug aacgacgccg ggugcaccua ccucggcgac      600

uacuucuaca agcuguacag cgagaacggc aaccgcgucc ggauccgcaa ccacuacacc      660

gcccggaacg agcacuaccu ggccgaguuc gaggcgaucu gccaggugca gaagcucgag      720

cccgagcugg uggaggcccu gcgccgcgcc aucuucuucc agcggccgcu caagucccag      780

cgcaaggggg ucggcaagug cacguucgag ccccggaagc cccgcugcgc cgacagccac      840

cccgacuacg aggaguuccg gaagcugugc uucaucaaca acaucaagau ccagaccucc      900

gacgacgaca ccaugcgccc gcugaacaag gaggagcggc agcgcaucaa cagccucuuc      960

uaccgggugu ccaaggcgaa cuucuacuuc gaggagaucg ccaagcugcu cgggggccgc     1020

aacaacuacu gcuacuucaa ggacgaggug cagaaggacu ucaaguucaa cuaccggaug     1080

agccaguccg ucagcggcug ccccaccacg gcccagcugc gcaacaucuu cggggaggac     1140

uggaagaccg gcaucgccga gaccuaccag cacagcgcca agaaggacgg gucccugaag     1200

accguggacg agauggcgga cgacaucugg aacgugcugu acagcuucuc cagcaaggac     1260

ugccucuaca acuucgccgu caacaagcug cagcuggagg aggacaaggc caagaaguuc     1320

uccaagauca agcucagcca cggcuucgcc uccaucagcc ugaacgccau ccacaagauc     1380

cugcccuucc uccggauggg gaugaucuac ucccacgcgg ugcuguucgc caacaucccc     1440

acgaucgugg gcauggacgu cuggaacgac gagagcaagc gccagcacau caugaccgag     1500

cuguccgacc ucguguucaa cuacgacccg aagaaccggg agguggaggg caccaucgag     1560

augugcauca aggacuuccu gcugaacaac uacgaccucc ccgccggggc cgucugccgc     1620

cuguaccacc ccagcaugau cgacaccuac cccgacgcca aggcgaacaa guacggcgug     1680

cugcagcugg gguccccggc cacgaacgcc auccggaacc ccauggccau gcgcagccuc     1740

cacgagcugc ggcacgucgu gaaccugcuc cugcgcaagg gcaagaucga ccagaacacc     1800

gaggugcacg ucgaguacgc ccgcgagcug aacgacgcga acaagcggaa ggccaucgcc     1860

gaccgccagc gggagcucga caagcugcac aagaaguacc acgacgacau caucacccug     1920

uacaaggagg agaccgggaa ggacaucaac cccacgcagg ccgacauccu caaguuccag     1980

cugugggagg agcaggagca cgugugccug uacaccggcc gccagaucgg caucuccaag     2040

uuccucgggc ccgacccgga guucgacauc gagcacaccg ugccccggag cgucggcggg     2100

gacuccaccc agaugaacau gacgcugugc gaccgccggu ucaaccgcga cgugaagaag     2160

gcgaugcugc ccagccagcu ccccaacuac caggagaucc ugccgcggau cgagaagugg     2220

aagaagaagu acgagaagcu gaccaaggac aucgaccacu gcaucaccca cuccggcaug     2280

accaaggagg ccaaggacag caucauccag aagcgccacc ggcucacgcu ggagcacgac     2340

uacuggcgca acaaguacag ccgguucacc augaccgagg ugcccgaggg guucucccgc     2400

cggcagggcg ccggcaucgg gcuggucagc aaguacgccg gccucuuccu gaagucccug     2460

uuccacgacc ccaccgaccg ccggcgcagc cacguguacg uggucaaggg gaccacgacc     2520

gccgaguucc ggaagaugug gggccuccag uccgaguacg agaagaaguc ccgcgacaac     2580

cacgugcacc acugcgugga cgcgaucacc aucgccugca ucggcaagaa cgaguacaac     2640

aagauggcca gcuucuacca cgacgaggag gccuucgagc gcggggagug cgagaagccc     2700

cacuucgaca agccguggcc caccuucacg caggacgucc ugggccugga gaagcggcuc     2760

aucguggugc acagcaccca ggacaacaug cccaagcacg ccaagaagua cguccgcacc     2820

ccgcggggga aguucaucgc gaagggcgac ugcgcccgcg ggucccugca ccuggacacc     2880

uacuacggcg ccaucaagca caaggacgac acggagcccc gguacgugcu ccgccgggag     2940

cugagcuccu ucgacagcgu gaacgaccug gacaucaucg ucgacgacgc cgugcgcgag     3000

aagaucaagg aggccgugaa gggcaagucc gggaaggcgu ucaaggaggc ccucgccaac     3060

gucuacauga acaaggagaa ggacauccgg aucaagaagg ugcgcugcua cgugcccagc     3120

gucaagcggc ccaucgacau ccgccaccag cgggacaugu cccgcuucga cuacaagcgg     3180

caguuccacg uggccaacga cggcaacuac augauggcga ucuacgaggg gguggucaag     3240

ggcaagcuga agcgcgacuu cgagcuggug aagaacaucg aggccgccag cuacuucaag     3300

gugucccagg acaacgagag cuucagcucc aucgucccgg agaaguccag caacggguuc     3360

ccccucaagg ccgugcugaa gaucggcacc cacgugcugc ucuacgagaa caccgccagc     3420

gagaucgacu ucuccgaccc cgucgaccug acccggcgcc uguacaaggu ggugggccuc     3480

agcuccgcga ucaucaacaa cugcagcuac ggguucauca cgcugcgguu ccaccaggag     3540

gcccgccagg ccaagaaccu gaagcucacc aagggcgucu ucaagaacgu ggacaacuac     3600

cgcggggcca ucauccugua ccacacccag cugaaggccc ucguggaggg cuacgacuuc     3660

gacaucaaca uccuggggga gaucaccucc aagcaguga                            3699


<210>  1738
<211>  3705
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1738
augcccaacu ucuggagcau cuucaccgag aaccugcaga ccuaccucau gaagacgcgc       60

auccugggcc uggacaccgg gaccaacucc cucggcuggg ccguggucga ccgggacgag      120

aacaaccagu acaccccgau cugccgcggg gaccugaucu ucacggaggg cgugaagcag      180

gagaagggga ucgaguucag ccuggccgcg gagcggaccu ccuaccgcgc cagccggcgc      240

cacuacuucc ggcgccggcu ccgcaaggug gagguccuga aggugcuggu gaagcacaag      300

cucugcccgc cccuguccga cgaggcccug aaccgguggc acaccaagaa ggaguacccc      360

cucgacgagg acuucauccu guggcagcgc accaacgagg ccgacaacaa gaaccccuac      420

cacgaccggc acaucugccu gcaccgcaag cucaaccuga aggacagcca gacggaccgg      480

uacacccugg gccgcgcccu cuaccaccug acccagcggc gcggcuuccu guccaaccgg      540

cucgaccaga acgaggaggc ggaggagacc gggaagguca agagcgccau cuccgagcug      600

agcaaggaga ugaaggaggc cggcugcgag uuccuggggg acuacuucua ccuccuguac      660

cgcacggagg gcaaccgggu gcgcauccgc ucccgguaca ccgaccgcga gaagcacuac      720

cugcaggagu ucaaggagau cugccgggug caggagcucg acgaggaccu ggccaaggcg      780

cuggagaaga ccaucuucuu ccagcgcccc cucaagagcc agcggcaggg ggucggcaag      840

ugcaccaugg agaagggcca cucccgcugc gccgugagcc acccccugua cgaggaguac      900

cggcugcucu gcuaccugaa caacauccgc aucaagacgc ccuccgacga ccggcugcgc      960

ccgcucaacg ccgaggagcg ggagaagauc acgccccugu ucuacaagga gaagaacuuc     1020

gacuucgaga agaucgccaa gaagcuggcc gggaagaccc ccuucggcug gauccgcgac     1080

gcggaggacu ggcccuacca guucaacuac cggaugaccc aggaggcccc ggccugcccc     1140

accaccgccc agcucaucaa gguguucggg gaggacugga aggccgcgcu ggccgagacc     1200

uacaccauga gcgccaagca ggacggcacg cugaagaccg aggacgagau cugcaccgac     1260

aucuggaacg uccucuacuc cuucagcucc aaggagaagc ugaaggaguu cggccugaag     1320

aagcuccagc uggacgaggu gcaggccaag gccuucagcg agauccgccu gacccgggac     1380

uucgcgagcc ucucccugaa ggccauccgc aagauccugu ucuuccugcg gacgggggag     1440

gacuacagca aggccaucuu ccucgccaac auccccacca ucgugggcga ggagaucugg     1500

aacgacaucg agcgccgguc ccgccugcug uacaaggucg accucgcccu gcgcccccag     1560

gaggagcggg acgcggagga gcaccgcacc aucgccgagc ggaucaaggc cgccguggcg     1620

gccgaggugc cggacgcccc caucgaccug cucuaccacc ccagcaugau cgaggccuac     1680

cccgacgcca agcgcaacga gcgguucgac ugcuaccagc uggggucccc gaagaccaac     1740

gcgguccgca accccauggc caugcggagc cugcacaugg ugcgcaaggu gaucaaccac     1800

cuccugcgga agcacaucau cgacgagaag acggagaucc acaucgagua cgcccgcgag     1860

cugaacgacg ccaacaagcg gcaggccauc gcggacuggc agcgcgagcu cuccaagcgg     1920

cacaccgccu acgcccagaa cauccgccag cuguacaagg ccgagaccgg ccuggucauc     1980

gagcccacca aggccgacau ccucaaguac cagcuguggg aggagcagca gcacgugugc     2040

cuguacacgg ggaagaagau cggcaucgcg gacuuccucg gcgccaaccc caaguucgac     2100

aucgagcaca ccaucccgcg gagccgcggg ggcgacucca cccaggagaa ccugacccug     2160

ugcgagagca aguucaaccg ggacaucaag cgcgacaagc uccccaccga gcuggccaac     2220

uacgaggaga uccuggcccg gaucgagcac uggaagaagc agugcuacgc gcucaagcug     2280

aagcgcgacg ggauccggac gcacuccggc cuggagaagu ccaucaagga cagccgcauc     2340

cgccggcgcc accucguggg gcuggaguac ggcuaccuga agggcaagua cgaccgguuc     2400

accaugaagg agguccccga gggguucagc cgccggcagg gcgccgggau cggccucauc     2460

uccaaguacg ccgggcuguu ccucaagagc cuguucaagg accccaagaa cccgaaccgc     2520

uccaacgugu acgucgugaa gggcgccauc accgccgagu uccggaagcu guggggccug     2580

cagcaggcgg acgaggccaa gagccgcgag aaccacaccc accacugcau cgacgccuac     2640

acgaucgccu gcaucgggcc cugcgaguac gccgcgcucg ccgccuacua ccgguccgac     2700

gaggaguuca aguacggccg ccggcgcgag aagccccagu ucgagaagcc guggcccacc     2760

uucaccgagg accugcugaa gcuccaggag gagcugcuga ucgugcacca gaccacggac     2820

aagcucggga agcgggaccg ccggaagguc aagacgcccc gcggcaaguu ccugaccggc     2880

ggggacagcg cccggggccg ccugcaccag gagaccuacu acggggccau caacuacgac     2940

ggcaacauca aguacguggu gcggaagccc cucgacagcc ugaccgagaa ggaccuggac     3000

aagaucgucg acgagaccgu gauggggauc gugaagggcg cggucgaggc caagggcucc     3060

cucaaggagg cccuggccga cgggaucuac augaacaagg agaagggcau ccugaucaag     3120

cgcgugcgca ucuucagcga cguguccgag ccgaucaacc ucaagcagcu gcgggacguc     3180

agcaccaagg aguacaagcg ccacauccac cuccggaagg acgagaacua ccuguucgcg     3240

aucuacgagg ggaucgacaa gaagaagaac aagaagcaga ccgagacggc caccguggac     3300

uaccuggacg ccgccaaguu cuacaaggac uccaagcgcc gggacaaggc caacagccug     3360

gugggcgacu acuccaagaa gaacaagcuc ccccugaagu gcauccugaa gaagcgccag     3420

augguccucu ucuacgagca cagccccgag gagauccggu ucaaggacaa gaaggaccug     3480

ucccgccggc uguacaucgu gaccagcauc gcgaaggacg ggcgcgugaa guuccuccac     3540

caccaggccg ccaccgccac gggcauccag uccagccccu ccccguucac caacgacggc     3600

gagcaccagc ccuacuaccg gcugagcgcc uccaacauca agguccuggu ggcggggauc     3660

gacuucgaca ucaacauccu cggcgagauc acccccaagc uguga                     3705


<210>  1739
<211>  3711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1739
auguacuucu acaagaacaa ggagaacaag cugaacaaga aggugguccu cggccuggac       60

cuggggaucg ccagcguggg cuggugccuc accgacaucu cccagaagga ggacaacaag      120

uuccccauca uccugcacgg ggugcgccug uucgagaccg ucgacgacag cgacgacaag      180

cuccugaacg agacgcggcg caagaagcgg ggccagcgcc ggcgcaaccg gcgccuguuc      240

acccggaagc gcgacuucau caaguaccuc aucgacaaca acaucaucga gcuggaguuc      300

gacaagaacc cgaagauccu ggugcggaac uucaucgaga aguacaucaa ccccuucucc      360

aagaaccucg agcugaagua caagagcgug accaaccugc ccaucggguu ccacaaccuc      420

cgcaaggccg cgaucaacga gaaguacaag cuggacaagu ccgagcugau cguccuccug      480

uacuucuacc ugagccuccg gggcgccuuc uucgacaacc ccgaggacac caaguccaag      540

gagaugaaca agaacgagau cgagaucuuc gacaagaacg agagcaucaa gaacgccgag      600

uucccgaucg acaagaucau cgaguucuac aagaucuccg gcaagauccg cagcacgauc      660

aaccugaagu ucgggcacca ggacuaccug aaggagauca agcaggucuu cgagaagcag      720

aacaucgacu ucaugaacua cgagaaguuc gccauggagg agaaguccuu cuucagccgg      780

auccgcaacu acuccgaggg ccccgggaac gagaaguccu ucagcaagua cggccucuac      840

gccaacgaga acgggaaccc cgagcugauc aucaacgaga agggccagaa gaucuacacc      900

aagaucuuca agacccugug ggagagcaag aucggcaagu gcuccuacga caagaagcuc      960

uaccgggcgc ccaagaacag cuucuccgcc aagguguucg acaucaccaa caagcugacg     1020

gacuggaagc acaagaacga guacaucagc gagcgccuga agcggaagau ccuccugagc     1080

cgcuuccuga acaaggacuc caagagcgcc guggagaaga uccucaagga ggagaacauc     1140

aaguucgaga accuguccga gaucgccuac aacaaggacg acaacaagau caaccugccg     1200

aucaucaacg cguaccacag ccucaccacc aucuucaaga agcaccugau caacuucgag     1260

aacuaccuga ucuccaacga gaacgaccuc agcaagcuga uguccuucua caagcagcag     1320

agcgagaagc uguucgugcc caacgagaag ggguccuacg agaucaacca gaacaacaac     1380

gugcuccaca ucuucgacgc caucagcaac auccugaaca aguucuccac cauccaggac     1440

cggauccgca uccuggaggg cuacuucgag uucagcaacc ucaagaagga cgucaagucc     1500

agcgagaucu acuccgagau cgccaagcug cgcgaguuca gcgggacguc cagccugucc     1560

uucggcgccu acuacaaguu cauccccaac cucaucagcg agggcagcaa gaacuacucc     1620

accaucagcu acgaggagaa ggcccugcag aaccagaaga acaacuucuc ccacagcaac     1680

cuguucgaga agaccugggu ggaggaccug aucgcguccc ccaccgugaa gcggagccuc     1740

cgccagacga ugaaccugcu gaaggagauc uucaaguacu ccgagaagaa caaccucgag     1800

aucgagaaga ucgucgugga ggugacccgg agcuccaaca acaagcacga gcgcaagaag     1860

aucgagggga ucaacaagua ccggaaggag aaguacgagg agcugaagaa ggucuacgac     1920

cugccgaacg agaacaccac ccuccugaag aagcuguggc uccugcgcca gcagcagggc     1980

uacgacgccu acagccugcg gaagaucgag gccaacgacg ugaucaacaa gcccuggaac     2040

uacgacaucg accacaucgu gccccgcucc aucagcuucg acgacuccuu cagcaaccuc     2100

gucaucguga acaagcugga caacgccaag aaguccaacg accugagcgc caagcaguuc     2160

aucgagaaga ucuacgggau cgagaagcuc aaggaggcga aggagaacug gggcaacugg     2220

uaccugcgga acgccaacgg gaaggccuuc aacgacaagg gcaaguucau caagcuguac     2280

acgaucgaca accucgacga guucgacaac uccgacuuca ucaaccgcaa ccugagcgac     2340

accagcuaca ucaccaacgc ccuggugaac caccucaccu ucuccaacag caaguacaag     2400

uacuccgucg ugagcgucaa cggcaagcag acguccaacc ugcggaacca gaucgccuuc     2460

guggggauca agaacaacaa ggagaccgag cgcgagugga agcggcccga gggcuucaag     2520

agcaucaacu ccaacgacuu ccugauccgc gaggagggga agaacgacgu gaaggacgac     2580

guccucauca aggaccggag cuucaacggc caccacgcgg aggacgccua cuucaucacc     2640

aucaucuccc aguacuuccg cagcuucaag cggaucgagc gccugaacgu gaacuaccgg     2700

aaggagaccc gcgagcugga cgaccucgag aagaacaaca ucaaguucaa ggagaaggcc     2760

uccuucgaca acuuccugcu gaucaacgcc cucgacgagc ugaacgagaa gcugaaccag     2820

augcgcuuca gccggauggu gaucacgaag aagaacaccc agcuguucaa cgagacccuc     2880

uacuccggga aguacgacaa gggcaagaac accaucaaga aggucgagaa gcugaaccuc     2940

cuggacaacc gcacggacaa gaucaagaag aucgaggagu ucuucgacga ggacaagcug     3000

aaggagaacg agcucaccaa gcugcacauc uucaaccacg acaagaaccu guacgagacc     3060

cucaagauca ucuggaacga ggugaagauc gagaucaaga acaagaaccu gaacgagaag     3120

aacuacuuca aguacuucgu gaacaagaag cugcaggagg gcaagaucag cuucaacgag     3180

ugggucccga uccucgacaa cgacuucaag aucauccgga agauccgcua caucaaguuc     3240

uccagcgagg agaaggagac cgacgagauc aucuucuccc agagcaacuu ccugaagauc     3300

gaccagcggc agaacuucuc cuuccacaac acgcuguacu gggugcagau cuggguguac     3360

aagaaccaga aggaccagua cugcuucauc agcaucgacg cccgcaacag caaguucgag     3420

aaggacgaga ucaagaucaa cuacgagaag cucaagaccc agaaggagaa gcugcagauc     3480

aucaacgagg agcccauccu gaagaucaac aagggggacc ucuucgagaa cgaggagaag     3540

gagcuguucu acaucgucgg ccgggacgag aagccccaga agcuggagau caaguacauc     3600

cucgggaaga agaucaagga ccagaagcag auccagaagc ccgugaagaa guacuucccg     3660

aacuggaaga aggugaaccu gaccuacaug ggcgagaucu ucaagaagug a              3711


<210>  1740
<211>  3717
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1740
augaccaagg ugcugggccu cgaccugggg gccgccagcg ucggcugggc gaugaucgag       60

gaggaggaga agucccacaa gauccugggg cucggcagcc gcaucauccc cuacgagggg      120

accgagggca acgacuucgc caagggcacg ggggaguccc ggaacgcccu gcgcaccaag      180

gcccggaccg cccgcaaggg cuacgaccgg uaccagcugc gccggaagua ccucguggac      240

acccugguga agaacgagau gaugccggac gaguaccugc gcaagcuccc caagaugcag      300

cugugggagc ugcggagcaa ggcggucacg accgagauca ccaagcagga gcucgggcgc      360

cuguuccugu ggcucaacca gaagcggggc uacaagucca gccgcuccga cgccaaccug      420

gacaagaagg acaccgagua cgugguggag gucaagagcc ggcacgagaa gaucaaggag      480

uccggguuca cgaucggcca guacuucuac agcgagcuga aguccaacga guacuuccgc      540

gugaaggaga acaucuuccc ccgggaggcc uacaucgagg aguucgacgc caucugcaag      600

cagcagaagc gccaccucac cgacaagcug aucagccgga uccgcaacga gaucaucuac      660

uaccagcggc cccugaaguc ccagaagggc cucgugagcg ucugccgcuu cgagggguuc      720

uucaagaagg gcaucaacgg gaaggaguac uucgugggcc cgaagguggc gcccaagucc      780

agcccccugu uccagaccgu caagaucugg gagaacauca acuccauccg gcugagcgac      840

cgcaacggca aggagaucau gcucucccug gagcagaaga agugcaucuu caaccaccug      900

gacaacaacg agaagcucac ccccaaggac cuguucauca ucaucggggu gaagaaggag      960

aacugcuucg ugaacaagca gcuggagaag ggcauccagg ggaacgucac caagagcacg     1020

auccucaagu gcuucggcga gaacaacgac uacgacaagu ggcugaagcu ggaccucaac     1080

aucaucguga agaaggacac cuucggguac cuguacgaca agaagaccgg cgagaucauc     1140

ggcgagaagc ugaucaagca cguggacagc gccgucgagc gcgagccgcu cuaccagcug     1200

uggcacacga ucuacuccau caacaacacc gacgagugca gcgccgcccu gcagaacaag     1260

uucggguucg acuccgagac cgccgacaag cucagcaaca ucgacuucgg caaggcgggg     1320

uucggcaaca aguccgccaa ggugauccgg aagauccugc ccuaccugau ggagggggac     1380

gacuacagcc aguccaugag cuacgccggc uucaaccacu ccgacagccu caccaaggac     1440

gagaaccuga agcgcaagcu gcucgacaag cugcggccca ucgagaagaa cucccugcgc     1500

cagcccaucg uggagaagau ccucaaccag auggucaacg uggugaacgc caucaucgac     1560

aaguacggca agccggacga gauccggauc gagcuggccc gcgagcugaa gcagaacaag     1620

gaggagcgga acaagacgga caaggacaug agcaagcgcc agcgggagaa cggggagauc     1680

uccaagcgcc uggcggagua cggccuccgg gccacccgca acaacaucau caaguggcgg     1740

cuguacgagg agaucgacaa ccaggacaag aagcugaacg ccaucugcgu cuacugcggg     1800

cagcccauca gccucaccga ggccaucaag ggcaacgagg uggacgucga gcacaucauc     1860

cccaagucca agcuguucga cgacagccag uccaacaaga cccuggccca ccgccggugc     1920

aacagcacga agggggacau gaccgcguac gacuucaugg agaacaagcc cgcccaggug     1980

uucaacgccu acaucgagcg cgugaacaug cucuacgaca acaagaucau ccggaagguc     2040

aagcgcgaca agcugcugau gccggagucc aagauccccg acaacuucau cgaccggcag     2100

cuccgcgaga gccaguacau cgccagcaag gcccgcgaga uccugcagac caucugcuac     2160

aacguguggu ccaccagcgg cacggugacc gcgcggcugc gccaccucug gggcugggac     2220

gacgccacca ugucccugca gcugcccaag uaccgggagc ucgggcugac cgagaugguc     2280

gagugggaga gcgagcacgg caagcagaag cacuccaagg aggugaucag cgacuggucc     2340

aagcgcgacg accaccggca ccacgccgug gacgcccuga cgaucgcgug caccaagcag     2400

ggguucaucc agcgcuucaa cacccucaac agcuccaaga ccaaggagga cauggaguuc     2460

gagaucgcca agugcagcau cgaguaccgg gagaaccugu ccaagcugga gaaguacauc     2520

aucgcccucc gccccauccc ggucagcgag guggaggacg ccguggccaa cauccugauc     2580

uccuucaaga gcggcaagaa ggucgcggug aagggcaagc ggaagaucgg gaagcgcggc     2640

aacaagaaga ucauccagac ggacaucauc aucccccacg ggucccugag cgaggagucc     2700

guguacggca agaucaagac caucgagcgg gagaagcccc ucaaguaccu guuccagaac     2760

agccagcuga ucuucaacaa caagaucaag auccucgucg aggagcgccu ggccaccuuc     2820

gagaacgaca ccaagaaggc ccuggccagc cucaagaagg ugcccgugua ccuggacgag     2880

gagaagacca ucguccugga guacgccacg ugcuucaagg aggaguacgu gaagaaguac     2940

gacguggaca ccaacuucuc caagaccgac aaggucaucg acgacaagau caaggagauc     3000

cuccagcacc ggcuggggaa guucggcggc aaggcgaagg agaaggaggc cuucaaggac     3060

gugcagcagg gggacaagac caucaagugg uacgaggacg agggccugga gcgcccgauc     3120

cacagcgugc ggugcuucac ggggcucucc gccaucgucc ccgugaagaa ggacgagaac     3180

ggcaucgaca ucggguucgu gaagcccggc aacaaccacc acaucgccau cuacagcgac     3240

aaggacggca accgcuacga gcacaucugc accuucuggc acgccgucga gcggaagaag     3300

uacgacaucc ccguggugau caaggacucc aacgagaucu ggaacaagau cuggagcaag     3360

ccggaggggu ccuaccccca gagcuuccug gacaagcugc cccaggagaa ccucacccug     3420

gaccugucca ugcagcagaa cgagaugcuc auccugggca ugagcuacga ggacaucaag     3480

accgcgaucg acaacuccga cuacaagcag cugagcucca accucuaccg ccugcagaag     3540

cuggccaacg ccaacuacau guuccggcac caccucgaga cgcagaucau cgacgacaac     3600

aacagcaagg ccucccgccg guucaucaac auccacagcc ugaaggcgcu guucgccugg     3660

aacccguaca aggucaacau caacugccuc ggggacaucc agaccaacuc ccgcuga        3717


<210>  1741
<211>  3720
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1741
augaccgaga agcgcaaguu cgacauccgg gagguguacc uggccaucca ccacaucguc       60

aaguaccgcg gcaacuuccu caacagcgcc accguggacu ccuucaagac gagcaacauc      120

gacuucaccu cccaguucga ccggcugaac gagcuguacc gccaggugau ccucgaggag      180

cccuuccaga ucaacaugaa ccaggucgac aagaugacca acaagcugcu ggacaacgac      240

gcgcucaagc uggacaccca gaagcagguc gccaagcugc ucccggugau cuacaacgac      300

aagcccgugg acaagcagua cacgcggcug gccaccgagu ucagcaaggc cauccugggg      360

uacaagacca agcucgacgu gauccugaac cuggacaccc agaacgccaa ggacugggcg      420

auccgccucg acgacgagga caucgacgac aagcugcccg cccuggugga gaaccucgac      480

gagucccggc aggagaucgu cacgaucauc cgcgaccugu acgcccagau cacccugaac      540

gccaucgugc ccaagggcaa gagccucucc gagagcaugg uggacaagua caacgaccac      600

aaggaccacc uggaccugcu cauggggcug auccacgagc ugggcgacga cuccgcgaag      660

gggaucaagc uccgggaggc cuacacccag uacgucggca agagcggcga caagacccug      720

aaccaggacg acuucuacgc cgccaucaag aagaaccugg acgaggacuc caagcgcagc      780

ccgaagaucc agcggcucau cgagcaggcc uccuucaugc ccaagcagcg cacgagcgcg      840

aacgggguga ucccccacca gcugcaccag cuggagcucg accggaucau cgagaaccag      900

ggccgcuacu accccuuccu gaaggagccg aacuccaaca cccacaagcc caacgggggc      960

aaguacaagc uggacgagcu cguggccuuc cggauccccu acuacgucgg gccccugauc     1020

accaagcagg accaggagaa gaccagcggc gccgacuucg ccuggaugau ccgcaagccg     1080

ggccagggcg gggagaucac gcccuggaac uucgaccagg agguggaccg gauggccucc     1140

gcgaacaccu ucauccgccg gaugaccacc aaggacagcu accugguggc cgaggacguc     1200

cuccccgacu ccagccugau cuacgagaag uucaaggugc ugaacgagcu caacaugcug     1260

aaggugaacg accgccggcu gagcaucucc cagaagcagg accucuucaa caaccuguuc     1320

aagaagcaga agacgauccg caccaagaag cugcagaccu acauccgcac caagugggag     1380

cuccccagcg ucaugaucuc cgggcugagc gacccggaca aguucaacuc cagccuggcc     1440

acguacaucg acuuccggga cguguucggc gagaaggugg acgaccccaa ccgccaggcc     1500

gacuacgaga agaucaucga gugguccacc gucuucgagg accggaagau cuaccaggcc     1560

aagcugaacc agcucgacug gcugaccgag agccagucca aggcgcugcu caccaagcac     1620

uacacgggcu gggggcgccu gagcaagaag cugcucaccg gccugaagga ccagaacggg     1680

gacuccaucc uggaccagcu cuggaagacc aacgacaacu ucaugcagau ccagagccgg     1740

gacgaguucg ccaagcagau ccacgacgag aacgccaagc aguuccacga ggccaacaac     1800

guggacgaca uccuggacga cgccuucacc agcccccaga acaagaaggc gauccgccag     1860

gucgacaagg uggugcagga cgucgugaag gccgugggcu acgcccccga caagaucgcc     1920

aucgaguuca cgcggucccc gcaggaccgc ccccagcgga ccuucucccg ccagcggcag     1980

cugcagagca ccuaccucaa cgucgcgaag gcccugauga aguccaaccu gaaccaggag     2040

cucaagagcg ugaucgacuc ccagcagacc cugacggaca agcuguaccu cuacuucacc     2100

cagcuggggc aggacaugua caccggcaag gagaucaacu ucgacgagcu ggugaacuac     2160

cagaucgacc acauccuccc ccaggccuuc aucaaggacg acagccugga caaccgcguc     2220

cuggugagcg cccccaucaa caacgccaag uccgacaacg ugccggucaa gaaguucggc     2280

gcgaagaugg gguacuucug gaagcagcuc gccgagaacc accugaucag caagcggaag     2340

cugaacaacc ucaccaccga ccccgacaag aucggcaagu ucacggccca gggguucauc     2400

caccgccagc ugguggagac cucccaggug auccggcugg ucgccaacau ccucggcaac     2460

gaguacgggc acgacggcac caccaucauc gaggugacgg ccaagaugaa ccaccagaug     2520

cgcaaggacu ucgaccugau caagauccgg gacgugaacg acuaccacca cgcgauggac     2580

gccuaccuga ccgccuacgu cggcgacuac cucuaccugc gcuaccccaa gcugcggagc     2640

uacuucgugu acggggacuu caagaagcuc aaggaccagu cccugaagau ccgcaacuuc     2700

aacuuccugc acgaccucac caacgacgag gccggcgaca agaucgugga ccaggagacc     2760

ggggagauca ucugggacaa gcacgagagc gucaagcagc ugaagaaggu guaccacuac     2820

aaguucaugc ugguguccca ggaggucuac acgcgccagg acgcccucuu caaccagacc     2880

aucuaccccg cgagcgacgc cgacaagcgg aagcugaucc cgaucaagaa caacaagccc     2940

guggacgugu acggcgggua cuccggcaac gucgacgccu acauggccau cgugcgcauc     3000

cacggcaaga aggaggacaa guacaaggug gucggggugc ccaugcgggc gguggccgac     3060

cugaagaagg ccgagagcaa gggccgcgac aacuaccuca acgccgucca cgaggugcug     3120

aagccgcagu ucaccaagaa gaagaaggac cggaagaccg gggagaucac ggagaugaug     3180

gacgacuucg acgugcugcu cggcaagguc uacuaccgcc agcugaucgu ggacggcaac     3240

aagaaguuca ugcugggguc cagcaccuac caguacaacg ccaagcagcu cgugcugucc     3300

gacaaggcga ugcagguccu gagcaaggac gacaagcuca agaaccagga cgagaaccag     3360

aaccugaucg acguguacga cgagauccug gagaaggugg accaguacuu cgagcucuac     3420

gacaucaaca aguuccggca gaagcugcac gacggccgca agaaguucau cgagcugccc     3480

gucgacaacg acuucaacgg gaagaagcuc aucuccuacg gcaagcgggc caccaucauc     3540

agcauccuga acgggcugca cgccaacgcc accaugucca accucaagua ccugggcauc     3600

agcagccccu ucggcaugcu ccaggugccc aacgggauca uccugucccc ggccagccgc     3660

aucugcuacc agucccccac gggccuguuc gagcggaagg ugaagcugag cgaccucuga     3720


<210>  1742
<211>  3738
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1742
augaucaucu acuucaucuu cgagaagauc gugaacacca uccuggucau gaagaagauc       60

gugggccucg accuggggac caacagcauc ggcugggccc ugaucaacgc cuacaucgac      120

gaguacaaca aggagcaccu cuacgggauc gaggcgugcg gcucccgcau cauccccaug      180

gacgccgcca uccuggggaa cuucgacaag ggcaacagca ucucccagac ggccgaccgg      240

accagcuacc gcggcauccg gcgccugcgg gagcgccacc uccugcggcg cgagcggcug      300

caccgcaucc ucgaccugcu gggguuccuc ccgaagcacu acuccgacag ccugaaccgg      360

uacggcaagu uccugaacga caucgagugc aagcuccccu gggugaagga cgagaccggg      420

uccuacaagu ucaucuucca ggagagcuuc aaggagaugc uggccgacuu caccgagcac      480

caccccaagc ugaucgcgaa caacaagaag guccccuacg acuggacgau cuacuaccuc      540

cgcaagaagg cccugaccca gaagaucucc aaggaggagc uggccuggau ccuccugaac      600

uucaaccaga agcggggcua cuaccagcug cgcggggagg aggaggagac cccgaacaag      660

cucguggagu acuacagccu gaagguggag aaggucgagg acuccggcga gcggaagggc      720

aaggacaccu gguacaacgu gcaccuggag aacgggauga ucuaccgccg gacgagcaac      780

aucccccucg acugggaggg caagaccaag gaguucaucg ugaccaccga ccuggaggcc      840

gacggguccc ccaagaagga caaggagggc aacaucaagc gcagcuuccg ggcgcccaag      900

gacgacgacu ggacgcugau caagaagaag accgaggccg acaucgacaa gaucaagaug      960

accgucggcg ccuacaucua cgacacccuc cugcagaagc cggaccagaa gauccgcggg     1020

aagcuggugc ggacgaucga gcgcaaguac uacaagaacg agcucuacca gauccugaag     1080

acccaguccg aguuccacga ggagcugcgc gacaagcagc ucuacaucgc cugccugaac     1140

gagcuguacc ccaacaacga gccccggcgc aacagcaucu ccacccggga cuucugccac     1200

cucuucaucg aggacaucau cuucuaccag cgcccccuca agagcaagaa gucccugauc     1260

gacaacugcc cguacgagga gaaccgguac aucgacaagg agagcggcga gaucaagcac     1320

gccagcauca agugcaucgc gaagucccac ccccuguacc aggaguuccg ccuguggcag     1380

uucaucguga accugcggau cuaccgcaag gagaccgacg ucgacgugac gcaggagcuc     1440

cugcccaccg aggccgacua cgugacccug uucgaguggc ucaacgagaa gaaggagauc     1500

gaccagaagg ccuucuucaa guacccgccc uucggguuca agaagaccac gagcaacuac     1560

cgguggaacu acgucgagga caagcccuac cccugcaacg agacccacgc ccagaucauc     1620

acccgccugg gcaaggccca cauccccaag gcguuccugu ccaaggagaa ggaggagacc     1680

cuguggcaca uccucuacag caucgaggac aagcaggaga ucgagaaggc ccugcacucc     1740

uucgccaaca agaacaaccu gagcgaggag uucaucgagc aguucaagaa cuucccgccc     1800

uucaagaagg aguacggguc cuacagcgcc aaggccauca agaagcuccu gccccugaug     1860

cggaugggca aguacugguc caucgagaac aucgacaacg gcacgcgcau ccggaucaac     1920

aagaucaucg acggggagua cgacgagaac auccgcgagc gggugcgcca gaaggcgauc     1980

aaccucaccg acaucaccca cuuccgggcc cugccccugu ggcucgccug cuaccuggug     2040

uacgaccgcc acagcgaggu caaggacauc gugaagugga agaccccgaa ggacaucgac     2100

cuguaccuca aguccuucaa gcagcacagc cugcggaacc ccaucgugga gcaggucauc     2160

acggagaccc ugcgcaccgu gcgcgacauc uggcagcagg ucggccacau cgacgagauc     2220

cacaucgagc ucgggcggga gaugaagaac cccgccgaca agcgcgcccg gaugucccag     2280

cagaugauca agaacgagaa caccaaccug cgcaucaagg cgcugcucac ggaguuccug     2340

aaccccgagu ucggcaucga gaacgugcgg ccguacagcc ccucccagca ggaccugcuc     2400

cgcaucuacg aggagggggu gcugaacagc auccuggagc uccccgagga caucggcauc     2460

auccugggca aguucaacca gaccgacacc cugaagcggc cgacccgcuc cgagauccuc     2520

cgguacaagc uguggcugga gcagaaguac cgcagccccu acaccgggga gaugaucccc     2580

cucuccaagc uguucacgcc cgccuacgag aucgagcaca ucaucccgca gagccgguac     2640

uucgacgaca gccuguccaa caaggucauc ugcgagagcg agaucaacaa gcucaaggac     2700

cgcucccugg gcuacgaguu caucaagaac caccacgggg agaaggugga gcuggccuuc     2760

gacaagcccg uggagguccu cagcguggag gccuacgaga agcuggugca cgaguccuac     2820

agccacaacc gguccaagau gaagaagcug cucauggagg acauccccga ccaguucauc     2880

gagcgccagc ugaacgacag ccgguacauc uccaaggucg ucaagagccu gcucuccaac     2940

aucgugcgcg aggagaacga gcaggaggcg aucagcaaga acgugauccc cugcaccggc     3000

gggaucaccg accggcugaa gaaggacugg ggcaucaacg acguguggaa caagaucgug     3060

cugccgcgcu ucauccggcu caacgagcug accgagucca cgcgcuucac cagcaucaac     3120

accaacaaca ccaugauccc cuccaugccc cuggagcucc agaagggcuu caacaagaag     3180

cggaucgacc accgccacca cgccauggac gccaucauca ucgccugcgc caaccgcaac     3240

aucgucaacu accugaacaa cgugagcgcg uccaagaaca cgaagaucac ccggcgcgac     3300

cugcagaccc uccugugcca caaggacaag accgacaaca acgggaacua caagugggug     3360

aucgacaagc ccugggagac guucacccag gacacccuga ccgcccucca gaagaucacg     3420

gucagcuuca agcagaaccu gcgggugauc aacaagacca ccaaccacua ccagcacuac     3480

gagaacggca agaagaucgu gagcaaccag uccaaggggg acagcugggc cauccgcaag     3540

uccaugcaca aggagaccgu ccacggcgag gugaaccugc ggaugaucaa gacggugagc     3600

cucaacgagg cccugaagaa gccgcaggcc aucgucgaga uggaccugaa gaagaagauc     3660

cucgcgaugc uggagcuggg cuacgacacc aagcgcauca agaacuacuu cgaggagaac     3720

aaggacaccu ggcaguga                                                   3738


<210>  1743
<211>  3741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1743
augcgccugg uguaccucgc ccuggcccac augaucaagu uccggggcca cuuccuguac       60

gagggggacc ucaaggcgga gaacaccgac guccaggccc uguucaagga cuucguggag      120

gaguacgaca agaccaucga ggagagccac cuguccgaga ucacggugga cgcccucagc      180

auccugaccg agaaggucuc caagagcucc cgccuggaga accucaucgc ccacuacccc      240

accgagaaga agaacacccu guucggcaac cugaucgccc ucagccuggg gcugcaggcg      300

aacuucaaga cgaacuucca gcucuccgag gacgccaagc ugcaguucag caaggacacc      360

uacgaggagg accuggaggg ccuccugggg gaggugggcg acgaguacgc cgaccuguuc      420

guguccgcca agaaccucua cgacgcgauc cugcugagcg gcauccucac cgucgacgac      480

aacuccacca aggccccgcu gagcgccucc auggugaagc gguacaagga gcacaaggag      540

gagcuggccg ccuucaagcg cuucaucaag gagaagcucc ccaagaagua cgaggagauc      600

uucaaggacg acacgaagaa cggguacgcg ggcuacgugg gggccgacaa gaagcugcgg      660

aagcgcagcg gcaagcuggc caccgaggag gaguucuaca aguacgucaa ggggauccuc      720

aacaaggugg agggcgccga cuacuuccug gacaagaucg accgggagga cuuccugcgc      780

aagcagcgga ccuucgacaa cggcuccauc ccccaccaga uccaccucca ggagaugcac      840

gccauccugc gccggcaggg ggagcacuac cccuuccuga aggagaacca ggacaagauc      900

gagaagaucc ucaccuuccg caucccguac uacgugggcc cccuggcgcg gaaggggagc      960

cgcuucgccu gggccgagua caaggccgac gagaagauca cgcccuggaa cuucgacgac     1020

auccuggaca aggagaaguc cgccgagaag uucaucaccc ggaugacccu caacgaccug     1080

uaccugcccg aggagaaggu ccucccgaag cacagccccc uguacgagac cuucacggug     1140

uacaacgagc ugaccaaggu gaaguacguc aacgagcagg gcgaggcgaa guucuucgac     1200

accaacauga agcaggagau cuucgaccac guguucaagg agaaccgcaa ggugaccaag     1260

gacaagcucc ugaacuaccu gaacaaggag uucgaggagu uccggaucgu caaccucacg     1320

ggccuggaca aggagaacaa ggccuucaac gccucccugg ggaccuacca cgaccugcgc     1380

aagauccucg acaagagcuu ccuggacgac aaggugaacg agaagaucau cgaggacauc     1440

auccagaccc ugacccucuu cgaggaccgg gagaugaucc gccagcggcu gcagaaguac     1500

agcgacaucu ucacgaccca gcagcugaag gagcucgagc gccgccacua caccggcugg     1560

gggcggcugu ccuacaagcu gaucaacggc auccgcaaca aggagaacaa gaagaccauc     1620

cucgacuacc ugaucgacga cggguacgcc aaccggaacu ucaugcagcu gaucaacgac     1680

gacgcccuca gcuucaagga ggagaucgcg cgcgcccaga ucaucggcga cguggacgac     1740

aucgccaacg ucgugcacga ccugcccggc ucccccgcca ucaagaaggg gauccugcag     1800

agcgugaaga ucgucgacga gcucgugaag gucaugggcc acaacccgac gaacaucauc     1860

aucgagaugg cgcgggagaa ccagaccacc gacaaggggc gccggaacuc ccagcagcgc     1920

cugaagcugc uccaggacag ccugaagaac cuggacaacc ccgugaacau caagaacgug     1980

gagaaccagc agcuccagaa cgaccggcug uuccuguacu acauccagaa cggcaaggac     2040

auguacaccg gggagacgcu cgacaucaac aaccuguccc aguacgacau cgaccacauc     2100

aucccccagg ccuucaucaa ggacaacagc cuggacaacc gcguccucac ccgguccgac     2160

aagaaccgcg gcaagagcga cgacgugccc uccaucgagg ugguccacga gaugaagucc     2220

uucuggagca agcugcugag cgugaagcuc aucacccagc ggaaguucga caaccugacc     2280

aaggccgagc gcggcgggcu gacggagaac gacaaggccg gcuucaucaa gcggcagcuc     2340

guggagaccc gccagaucac caagcacguc gcccaggugc uggacgcgcg guucaacgcc     2400

aagcacgacg agaacaagaa ggugauccgc gacgucaaga ucaucacccu gaaguccaac     2460

cucgugagcc aguuccggaa ggacuucaag uucuacaagg ugcgcgagau caacgacuac     2520

caccacgccc acgacgccua ccugaacgcc gucaucggga ccgcgcugcu caagaaguac     2580

ccgaagcugg ccuccgaguu cguguacggc gaguucaaga aguacgacgu gcgcaaguuc     2640

aucgccaaga gcgacaagga gaucgggaag gccacggcca aguacuucuu cuacuccaac     2700

cugaugaacu ucuucaagaa ggaggucaag uucgcggacg gcaccguggu ggagcggccc     2760

gacaucgaga ccagcgagga cggcgagauc gccuggaaca agcagaccga cuucaagauc     2820

guccgcaagg ugcucagcua cccccaggug aacaucguca agaagacgga ggugcagacc     2880

cacgggcugg accggggcaa gccccgcggg cuguucaacg ccaacccguc ccccaagccc     2940

aagcccgaca gcuccgagaa ccucgugggc aucaagcgga accuggaccc gaagaaguac     3000

ggcggguacg ccggcaucag caacuccuac gcgguccugg ugaaggccau caucgagaag     3060

ggggugaaga agaaggagac caugguccuc gaguuccagg gcaucagcau ccuggaccgc     3120

aucaccuucg agaaggacaa gcgggccuuc cugcucggga agggcuacaa ggacaucaag     3180

aagaucaucg agcugcccaa guacucccug uucgagcuca aggacggcag ccgccggaug     3240

cuggccucca uccugagcac gaacaacaag cgcggggaga uccacaaggg caacgagcug     3300

uucgugcccc agaaguucac cacccuccug uaccacgcca agcggaucaa caaccccauc     3360

aacaaggacc acaucgagua cgugaagaag caccgcgacg acuucaagga gcuccugaac     3420

uacguccugg aguucaacga gaaguacgug ggggcgacca agaacggcga gcggcucaag     3480

gaggccgugg ccgacuucga cuccaagagc aacgaggaga ucugcacguc cuuccugggg     3540

gccgucaaca gcaagaacgc cggccuguuc gagcucaccu cccugggcag cgcguccgac     3600

uucgaguucc ugggggugaa gaucccgcgc uaccgggacu acacccccag cagccuccug     3660

aaggacucca uccugaucca ccagagcauc accggccucu acgagacgcg caucgaccug     3720

uccaagcugg gggaggacug a                                               3741


<210>  1744
<211>  3747
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1744
augaagaaga uccugggccu cgaccugggg agcgccucca ucggcugggc cgugaucacc       60

gagaacgucg agaacgggau caccaagugc gacauccugg gcauggggag ccgcaucauc      120

cccuacgagg gcacggaggg caaggacuuc accaagggga ccggcgaguc ccggaacagc      180

cuccgcacca aggcgaagac ggcccggaag ggguacgacc gcuaccagcu gcggcgcaag      240

uaccuggugg acacccucau caagaaccag auguacccgg acgaggccac ccggaaccug      300

cccaagauga agcuguggga gcuccgcgac aaggccgugc acucccccgu cagccugaug      360

gagcugggcc ggcuccugcu guggcucaac cagaagcgcg gguacaaguc cagccggucc      420

gacgccaacc ugggcaagaa ggacaccgag uacguggcga cggucaagag ccgccacgag      480

aagaugaagg agcuggacgu gaccaucggc caguacuucu accgggagcu cuccgccaac      540

gaguucuuca aggugaagga gaacaucuuc ccccgcgagg ccuacaucga ggaguucgac      600

gccaucugca agacccagca gaucgcgcac ccgaugaucc ugcggagcga gcugaucgac      660

aagauccgca acgaggugau cuacuaccag cggccccuca agucccagaa ggggcugguc      720

agcgugugcg aguucgaggg cuucuggcgc aucgggaagg acggcaagga guacuucgug      780

ggccccaagg ucgcccccaa guccagcccg cuguuccagc ucgccaagau gugggagaac      840

aucaacaaca ucaagaucgg gucccggacc ggcgaggacc ugaagcugac gaccgagcag      900

aagugggcca ucuuccagca ccucgacaac aacgagaagc ugacccccac cgaccugcuc      960

aagauccuga accugaagaa ggagaacugc uacgugaaca agcagauggg gaagggccuc     1020

aacgggaaca ucacgaagca cgccauccug cagugccugg gcggcagcga caaguacgag     1080

aaccuccuga agcuggagcu caagauggug gacggggaca aggagggcua ccuguacgac     1140

cgcaagaccg gggagguccu gaacuccaag augaucaagu ucaucgaccc caugguggag     1200

caggagcccu ucuaccagcu cuggcacauc aucuacagca uccacgacca ggaggagugc     1260

agccgggugc ugcaggcgaa guucgacauc gagcagccga ucgccgacaa gcuggccgcc     1320

aucgacuucu ccaagcacgc cuucggcagc aaguccgcga aggucauccg caagauccuc     1380

cccuaccuga uggacgggga cggcuacagc gaggccaugu ccuacgccgg cuacgaccac     1440

agcaacuccc agaccaagga ggagaaccug cagcgcaagc uccuggaccu gcugaagccc     1500

auccccaaga acagccuccg gcagccgauc guggagaaga uccugaacca gauggugaac     1560

gucgugaacg ccaucaucga gaaguacggg aaccccgacg agauccgcgu ggagcuggcc     1620

cgggagcuca agcaguccaa ggaggagcgc aacgacgcgg acaaggccau gagcaagcgg     1680

cagcgcgaga acgacgccau caccaagcgg cuggaggagu ucggccugcg cgccacgcgg     1740

aacaacauca ucaaguggcg ccucuacgag gagaucgaca accaggagaa gaagcugaac     1800

gccaugugcg ucuacugcgg gcagcccauc ucccugaccg aggcgcuccg gggcaacgac     1860

guggacaucg agcacaucau ccccaagagc aagcuguucg acgacuccca gagcaacaag     1920

acccuggccc accgccacug caacaagaac aagaacgacc ucaccgccua cgacuucaug     1980

aaggggaagc cggaggccgu cuucaacgag uacguggagc gggugaaccu gcuguacgac     2040

aaccacauca uccgcaaggc gaagcgggac aagcuccuga ugacggaguc caagaucccc     2100

gacaacuuca ucgaccgcca gcugcgggag agccaguaca ucgccaagaa ggcccgcgag     2160

auccuccaga ccaucugcca cgacgucugg uccaccagcg gcaccgugac ggccgagcug     2220

cggcaccugu ggggcuggga cgacgugacc augaaccucc agauggccaa guaccgcgag     2280

cuggggcuga cccaccaggu cgagugggag uccgagcacg gcaagaacaa gcacgagaag     2340

gaggugauca ccgacuggac gaagcgcgac gaccaccggc accacgcggu ggacgcccuc     2400

accaucgccu gcaccaagca gggguucauc cagcgcuuca acacccugag cgccgccaag     2460

acgcgggagg acaugauguu cgacaucgag cagcgcagcc acgaguucaa ggagaagcgg     2520

ucccugcucg agaaguacau caucagcgag cagccccuga aggucgacga gguggagaag     2580

gcggugucca acauccugau cagcuucaag uccggcaaga aggucgccgu guggggcacc     2640

cgcaagaucg ggaagcgggg cagcaagaag gugauccaga agaagaucau cgucccccgc     2700

gggucccuca gcgaggaguc cguguacggc aagaucaaga agcaggagga gaaggacuac     2760

aaguaccugu ucgagaaccc gcagcugauc uucaacccca ccaucaaggc caagguggag     2820

gagcggcucc ugauguucga cggggacguc aagaaggcca ucaccagccu gaagaagauc     2880

cccaucuacc ucgacaacaa caagaccgug gugcuggaga aggccaucug ccugaaggac     2940

gaguucguca ucaaguacuc cguggacauc aacuucaaca agguggacaa ggucguggac     3000

cccaagauca aggagauccu ccagaaccgc cugaagaagu ucggcggcaa ggagaaggag     3060

gcguucaagg acgugcagcu ggaggacaag augaucauca agugguacga ggacgagggg     3120

cucgagcggc cgaucaagag cguccgcugc uucacgggcc uguccgccgu ggugcccguc     3180

aagaaggacg agaacgggaa cgacaucggc uucgugaagc ccgggaacaa ccaccacauc     3240

gccaucuaca ccgacaccga ggagaacaag cagcagcacg ugugcaccuu cuggcacgcc     3300

gucgagcgga agaaguacga caucccggug aucaucaaga acacgaacga ggugugggac     3360

aagauccaga gccagcccga cggcaccuac cccgacuccu uccuggagau gcugcccccg     3420

cccaaccuca accugcucgu caacaugcag cagaacgaga uguucauccu gggcaccacc     3480

acggaggaca ucaagcuggc gaucgacaag cgcgacuaca agaucaucgg ggacaagcuc     3540

uaccggcugc agaagaugag caucaagccc uccagcggcc agaucgacau cguguuccgc     3600

caccaccugg agacccagcu caucgacgac gagaacagca agaaguccaa gcgguucuac     3660

aacgugcaga gccugggggc ccuguucgcc cucaaccccu ucaagguccg caucgacaac     3720

cugggcaacc uggugaagga gaaguga                                         3747


<210>  1745
<211>  3762
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1745
augaaggccc gcggcaacuu ccugcaccag gacacccccg ggcucaccgc cgcgaacgcc       60

aacaugggcg acgccgugga gaagcugugc gucgcccugg aggacuggug cacgcuccgg      120

aaccuggagu gcaacauccg caagaaccag aagaacaucc agaaccugcu cgaggggaag      180

accguggagg ugagcgccga gaacgcgcug aaggaguccu ucgacagcuc ccgggccagc      240

cgcaaggaca aggccaccau ccuguccacc uggaucgcca uccagccggg cgaguaccuc      300

gacgcgaagc aggccaagga cgccagcaag gagaucgcca acguccuguu cgggcugcag      360

ggcaacauga agaagaucuu caucgaguuc gaggaggacc agaagauccg gcucgacaac      420

gacgagcagg ucgaggccuu cuacucccug ugccccgacg acggcguggc gcuguucgag      480

agcuuccagu ccaucuacag cgccuacauc cucaugggga uccugaagaa cgccaacggc      540

ggguccauca gcuccugcaa gguggccgcc uacgaccagu acaaggagga ccugaagcuc      600

cugaaggacc uggugcgcga guucgcgagc gagaaguacg acgaguucuu ccggggcgac      660

aucuucgacu ccagcaaggg guuccgcgcc cggaacuaca accccgccac ggccaagggc      720

uacaccaagu acaacuccgu gcgcggcgcc agcuacgacg acuucaagaa ggaggucgag      780

aagaucuuca aggggaccgu ggcggaggag gacauccggu acguggacau gaugcagcgc      840

uucgccgagg gcaccuuccu ccggcgccug aagacguccg acaacgggag cauccccuac      900

cagcugcacc ucgaggagau ggacgccauc gucgagaacc agaagcaguu cuacccguuc      960

cuggagagcg agaaggagaa gcuggagucc cucgugaccu uccggaucag cuacuacgug     1020

ggcccccuga cccagaagaa cgcccgccug gacaaccaca acaacgcccg guucgcgugg     1080

ucccagcgca aggagggcaa ggagcacgcc cgggucuacc ccuggaacug ggaggaggug     1140

aucgaccgca agcagagcgc cgccaaguuc auggagcggc ucaccgggac gugcaccuac     1200

auccuggacg agcccgugcu gccgcgcugc ucccuccugu acgaggagua cugccugcuc     1260

aacgagcuga acggcagcaa guggcgcgac augggcgggg acuggcaccg guucgacuac     1320

gagacccgca uggagaucau cgagaaccug uucaagaaga agaagggguc cgucaaguac     1380

gcggccgugg aggacuggcu caagugcgag aagcacaaga gcugcgccga ggugaagggc     1440

ggccaggggg agaccggcuu cgaguccaag cugagcuccu acgucuucuu caaggaggug     1500

cugcacgugg acgagaucga cgaggccgac auccccauga ucgaggagcu caucuggugg     1560

aacacgcugu ucgaggaccg gagcauccug aaggagucca ucgaggacaa guacggggac     1620

cgccucaccg acgaccagau caaggccauc ugcaagaagc gguucaccgg cugggggcgc     1680

cugagcaagg agcugcucug cggcaucaag acccacacgg acaacggccc cuacuccauc     1740

auggacgucc ugcgggaggg gaaccccaac aacaacgcgc gcagcgagac caugguguuc     1800

auggagaucc ugcgggacga caaguucggc uucgacaagc ugaucgagga ggagaacaug     1860

cgccgggucu ccgaggugga caacuucgac gugaacgacc ucccggggag ccccgccguc     1920

cgccggaccg ugaaccaggc caagcgcauc guggacgaga ucgucggcau cgccgggugc     1980

gagccccaga acaucuucau cgaggugacc cgggacgagg acggccgcaa caagggccgg     2040

cgcacgaagc ggcgcuacga caacaucaag aacgccaucg cggcccugaa gaaggagcag     2100

aaggaguacu ucaacaagga cguguccgac agccugaaca agaccaaccc cagcgaccuc     2160

gacaacgagc gccuggcccu guacuucaug cagaacggga agugcaugua cugcggcaag     2220

gaccucgaca ucaagcagcu ggacaaguac caggucgacc acauccugcc gcaguccuac     2280

gugaaggacg acagcuucga gaacaaggcc cucgugcaca uggaggacaa ccagcggaag     2340

uccgccagca ugcugcugga cgacuccauc auccgcaaga ugaagcccua cugggaggcg     2400

cuccgggccg ccgggcugau cggcgagaag aaguucaaga accugacccg cguccgggug     2460

agcgacaagg agcucggcgg guucaucgcc cgccagaugg uggagaccuc ccagagcaug     2520

aagcugacgu cccugguccu cgagaagacc uaccccaaca ccaacgugcg gcccgugaag     2580

gcgggcuuca gcuccgaccu gcgcaagcgg cuggaccuca ucaagugccg cgagaucaac     2640

gacuaccacc acgcccacga cgccuaccug gccugcgaga ucgggcaguu caucaccaug     2700

cggcacaaca agcuguacga gaacccgauc agcuacuccc acgccgucaa ggacuucauc     2760

gcgaagcgcg ccaaguacgc caacgaccac ggcacgccca ugagcaccua cgggaugccc     2820

ggcuccagcu ccuucgugau cagcagcuuc cucaccuccg gcuucgacga ggagaccggg     2880

gagaucuuca aggacgccug gagcgccgag guggcggccg uccggauccg cgagucccug     2940

aaccggaagg acugcuacau cagccgcaug cccgaggaga cguccggcgc cuucuggaag     3000

gagacccuga ucagcccgcg ggaccccucc cucgggaaga accugagccu gcccaucaag     3060

aagggccucg acccccgcaa guacgggggc uacaacgagc agaaguucgc cuacuucuuc     3120

guguacgagg ccgugaagau caagaagaag ggcgagguca aggaguacgu guucgcggcc     3180

gugccggucc agguggccgc caucguggcg cgggacccca acgcccugac cggguucgcc     3240

aagaaggacg ccgaggcccg caagaucaag uuccugcgga ucauccgcga gaaggucuac     3300

aaguaccagc ucaucgaggu gaacggcgag cgccuguaca ucaccgggcu gaaguccgug     3360

cggaacgcgc gccagaucgc ccucagccag gacaugacca cggucuuccu gaaggccauc     3420

aacgacaagg acguggccga ggacgagcug aagucccucu ucgaggagau cgugcagcgg     3480

uaccgccugu gcgccaaccg gcugggcaac cagcucaaga ucuucagccu guccgacagc     3540

uucaacuccg cgaacagcga guccaagaag cgcgcccugg ucagccucgu ggccaucauc     3600

gacgccaaga ccaacaagau cgaccugucc gggaucggca gcggcaaggc cguggggaac     3660

augcggcuga gcuucuccaa ggagaugagc aacuccaaga accacuucgc guucaucgac     3720

cagagcguca ccggcauguu cgagcgcaag accuaccucu ga                        3762


<210>  1746
<211>  3777
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1746
augaccgagg agaaccccga cauccgcaag guguuccugg ccauccacca cauccucaag       60

aaccggggcc acuuccugcu gcaggggcag agcuucaagg agggcaaccu caacaaccug      120

aucaaggagc ugcucaaccu ggacauccug cacaucucca ucgagaucac cgaggagaac      180

aucaacaaga ucacggagau cgcccucgag aagaagaccc ugaccgacaa ggucaacgac      240

cugaagaaga ucuacccgaa ggagaagcag cuccuggagg cguuccgccu gaucuucggg      300

ggcaagacca gccucgacaa gcuguucgcc aucgacgagu acaaggagcu ggacgccucc      360

gucaagagcg uguccuucaa ggagaagauc uacgaggagg ugcggcacga cuacgagcag      420

guccucagca acuacaucga gcugcuggac cucgccaagc ugguguacga cuccaucauc      480

cugagcgaca ucaagaagga ggggaagacg cucuccgaga gcaaggugga gcuguaccac      540

aagcaccgcg aggaccugac caagcucaag acccugguga agaacgacuc caagcugagc      600

gaggacaaga agguggagcu cuacuccgcc aucuucaagg aggacaagga caagggcacc      660

aacuacguca acuacauccg caagagcgag gagggcaagg ggugcaacua cgaggacuuc      720

aagaaguucc uggugaagga gcuggcgaag cucgaggagu ccagcgccaa ggaggagauc      780

cugcgggacc uggagcucga gcuguuccug ccccuccagc ggacgaagga caacuccgug      840

guccccuacc agauccacaa ggaggagcug gugaagaucc uggacaacgc cgccaaguac      900

cacaaguucc ucaacgagaa ggacgacagc ggcuacucca ucaucgagaa ggugauccag      960

cugcuggagu uccgcauccc cuacuacguc gggccgcuca acagcagcaa gaaggcgaag     1020

guggacggcu ucgccugguc cgugcggaac aagggguacg agaacacccc cgucaccccc     1080

uggaacuacg agaaggugau cgacgagagc gccuccgccg agaaguucau caccaaccug     1140

acgaacaagu gcaccuaccu gaagggcgag gacgugcucc ccaagagcuc ccugcuguac     1200

agcgaguucg cccugcucaa cgagcugaac gcgcugaagu acgacggcaa ccgcaucucc     1260

cucgaggccc ggaacgucau caucgagaag cuguucaagg agcaggggaa gaaggugacc     1320

aagaccagca ucaagaaccu gcucaaggcc gagggcuaca ucgacgggaa gggcgagauc     1380

acgggcaucg acaucaccgu gaagaacgac cugaaguccu accgcgacuu caagaagauc     1440

cugggggaca aguucaacac cgagcacguc gagaacauca uccucuggau cacccuguac     1500

ggcgagagcc ggaagcugau caaggccaag aucgaggccg uguacgggga caucuacucc     1560

aaggaggaga ucgcgaagau gagccgccuc gucuacaagg acuggggccg guucucccgc     1620

aagcugcuga cggagcucgu gagcaagaag cuguacaacg aggagaccgg ggagugccug     1680

aacaucaucg gcgccaugcg gcagaacaac auccucuuca uggagcugcu ggccgaccgc     1740

uucgacuacu cccagcagau caucgacuac aacaaggagc uccagaagga ggugaccgag     1800

aucaccccgg agauccugga cgaccuguac gucagccccu ccgugaagcg gagcaucugg     1860

cagacggugc gcaucgucga ggagcucaag aagaucaucg gcugcgcccc cgccaagauc     1920

uucguggaga ccacccgguc caaccaggac aagaagaagc ccaccgacag ccgcaagaag     1980

cagcuggagc uguucuacaa ggcggugaag aaggacguca aggaccucga gaaggagauc     2040

gggagccuga acuucgacaa gcugaacgag cgccucucca gcguggagcc guccaagcug     2100

aaggccaaga agcuguaccu cuacuacacg cagcugggcc ggugcaugua cagcggggag     2160

accaucgaca ucacccagcu gaacaccacg gccuacgaca ucgaccacau cuacccccag     2220

uccaagguga aggacgacag cuucaccaac accguccucg ugaagcgcga guacaacgcc     2280

gccaagaccg accgguaccc ccugggcucc gacauccaga cgcccagcaa ccgccgguuc     2340

uggaaguucc ugaaggagaa ggggcucauc accgacgaga aguacaaccg ccuggugcgg     2400

accgaggagu ucuccgacga ggagcugacc ggcuucaucg cgcgccagcu cgucgagacc     2460

agccaguccg ugaaggccgu ggccggcauc cugggggagc ugaacccgaa gacgagcauc     2520

ugcuacucca aggccgagaa cgucagcucc uuccggcaga acuucggcaa gaucaaggag     2580

gggaaccgca agagcgacaa caccgagcag cucaucaagg ugcgggagau caacgacuac     2640

caccacgcga aggacgccua ccugaacgug gucgugggca acguguacga caccaaguuc     2700

acccgcaacg ucuucaacuu caucaaggac aagaaggacg ggcggaagua cucccugaac     2760

aacguguucu acgagaacgu gagcaacagc aacaacgucg ccugggagcu caacaagacg     2820

gugcaccagg uggagaaggu caugaacaac aacaacaucc uggugacccg ccggaccucc     2880

gagcagaagg gcggccuguu cgacgccacc guguacaagg ccaaggucgc ggccaaggcc     2940

aaggaggggg uguacuaccc ccucaagacg agcgaccccg uggucaacga cgugaccaag     3000

uacggcgggu ucaccaaggu gaagaucgcc uacuacucca ucuucgagua cacccugguc     3060

aacaagaagg gcgaggagaa gaucacgcgc aucaucccca ucccgaucua cgugagccag     3120

aacaucaagg acgacuccac ccugcucgag uucggcaaga cccagaucaa caucaagagc     3180

ggggaggagg ugaaggacuu caagcugaag uaccggaagc ugugcaucgg cgaccucguc     3240

aagaucaaca acuucuucaa cuucaucggg ggcaagacca acgacuccuu cguguacgac     3300

ggggccgugc aggucacgcu ggacaccgag agcgagcgcu acaucaagac ccuguccaag     3360

uaccagaacu ggaagaagga gaacaaggac ggcgagcucu gggagagcau caccaaggag     3420

aagaacacgg agcuguacaa ccugcucgug gagaagauga acuccggcau cuucaucaag     3480

aagaagccca acaaguucaa cgagcugaac agcgaggaga uccggaacaa cuucuccagc     3540

aucucccugg aggagcaggc gaaggugcuc cuggaggucc ugaaccuccu gaccaacaag     3600

aaguccaucu ucgaccucaa gagcaucaac aucaccgccg cccgcgggaa gaucagcuuc     3660

aaccugucca accugagcca guucagcauc aucgagcagu ccguggccgg cuucuacgag     3720

aaggagauca ccaucaucgg ggacaaggag aacgacaugg agaacaacaa cugcuga        3777


<210>  1747
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1747
augaaguaca uccugggccu cgaccugggg guggccagca ucggcugggc cgucaccgag       60

aucaacgaga agggggagcc cgugggccug aucgacgcga acuccgugau cuucaagccg      120

cucgacaacg acaaggggaa gcuguacaac gucgagcgcc gggacaagcg cggcagccgg      180

cgcauccugc ggcgcaagaa gcaccgggug gagcgcacca agaugcuccu ggugaacucc      240

agcuuccuga cgaacgagga gaucgacaac cucuacgucg gcaagcugga caacaucuac      300

gaggugcggc ugaaggggcu caaggagcag cuguccaaga acgagaucgc ccgccugaug      360

aucuacuacu gcaagaaccg gggcuucaag agcaaccgca agaccgagga ccucgagauc      420

cugaaguccc ugggggagaa gaucagcgag aaggacuccg acgagaagaa gcucaagccc      480

aucaucgcca agaacaccga gcugaucgag agcaagggcc ugacccccau cgaggugauc      540

uacaugaucc gggagaacga caacuccauc cucggguuca agaacaagga gggcaacuac      600

aaguucggcu ucaagcgcaa ccaggucauc gacgaggugc ggaagauccu ggggacgcag      660

cagauccuga gcaaggagau cguggacgag uacaucgaca uccucuccag ccagcgcgac      720

uucuccgacg gccccggggg cgacagcaag uacaagaucg acuacaccaa gcuggccggc      780

aagugcaagu acaccgggga gguccgggcc gugaaguccg cgccgagcua cgagaucuuc      840

accaugcugc agaagcucaa cgacauccgc uacgugaagu ucacguccga ggacaagauc      900

gagaagaaga agcugagcaa ggaggucauc cacaagcugu acgaccucgu gguggagaag      960

aacaagaccc ugaccuacga ccugaucgag aaguccaucg acgaggagaa caucaagcuc     1020

cugaacaucc ccaagcugag caagagcaag uacgucgagc uccggaaguc cuacuucaag     1080

gagcgcaacg acaacgagaa ccugaccgag gaggagauca acgccuucaa cgagaagcug     1140

aacaacgagc ggaugaagca ggagcucauc cgcaacaacc ugaaggccua caaggagcuc     1200

aagagcaagu ucaagaagaa caacaucgac gagaaccgca ucaaggagcu gggcgggauc     1260

uacuuccugg accugguggc cgagcuccug acguacucca agaccgacga gaagcuggag     1320

uacuucgugg agaacgucga gcgguacagc cucuucaagg agcagcgcga caucguggag     1380

aucaucaaga ccuuccccaa cuacaccaag aacggcaacc ugucccugag ccuggugcgg     1440

gagcucaaca agcugcugau ggaggggcac gacuacgagu ccagccucuc cagccugaac     1500

uacuacaucg acacggacgu cgacugggag aaguucccca ccaucuccga gaucgaggac     1560

agccugucca ccaagaucac caacccgaac gugaagcaca uccucgucau ccugcgcaag     1620

cuguacaaca cgcuccuguu caaguacggc cggcccgaga aggugcaccu ggagcucagc     1680

cgcgacuucu ccaacgacuu cagcacccgg aacaagaucc agaaggagca gcuggagaac     1740

aaggugcgcc gggaggucgc ggccuucgag auguacggcg ccaacaagga caucguggcc     1800

gggaaggacc gccuguccaa cgacgacuuc gugcggauca agcucuggga ggagcagaac     1860

aaggucugca uguacagcgg ccgcaccauc gagaaguacc agcugaccag cgccgaggug     1920

cagaucgacc acauccugcc cuacuccaag agcuucgaca acuccuacag caacaaggug     1980

cucgucuucu ccaacgagaa ccaggacaag aaggagcgga cgcccuacca guggcugaag     2040

gggaccgaga aguggaacga guucaagcag cgcgugcggc ugaaccucaa caucagcaac     2100

aagaagaagg agaaccugcu guucgaggac gaggugguca acaacgaguu ccucgagcgc     2160

gagcugcacg cgaccuccua cagcucccgg cuggcccuca acaucuucca gcgccugauc     2220

ccggugagcg acgaggacaa guacgacgag cacggcaacg agaaggugaa guaccuguac     2280

aaccggaacg ucaucgccuu ccaggggaag augaccucca ugcuccgcaa ccuguacagc     2340

cugaacaagu acacgcacuc cuucgagagc gacacccucg acaucgacaa caaggccuuc     2400

auccugaagg aguucgagau caacaaggac aaccugaaga ugaccgccua caacgugaac     2460

accggccucg agaucacguc cgagaccaag guggagaaga acaagagcgg cgaguucaag     2520

accaucaagg acgagcugcu gaagaaggag cucgacaaga aggagaaccu gaucgagauc     2580

uccgacuacu ucaaggacaa gguccuguuc gaccucaaga ucguggacuu cgaggagauc     2640

gacaccgugg agcccgagau caucagcauc cuguacaaga ugaucacggc gcugaaggag     2700

gaggucuaca gcaagaaccg cgagaaccac cuccaccacu cccuggacgc cuuccugcuc     2760

accaucauga accggagcau gcagaugaag cugaucaagu acaaccagcu gaucuccgug     2820

cucaagaaca aggagaucga gaucuucaac gaggagaagg gggaguacau cgacagcaag     2880

gaguucgccg aggagcugca gaagcagaag aucaucgaca ucgacggcaa cgagcgcggg     2940

aucaaguuca acgugaacgg caagacccac cggcugaacc ucgucaagcc cuacgagaac     3000

uuccuggacg acaucaagaa caagaucuuc gacaagcgcg agaacaacaa gcugcccuac     3060

cacgugguga aguccaaggu cagcggggcc cuccacgccg agaccauccu gggcgagucc     3120

aagggcgaga ucaccaagcg gaucagcgug uucgacauca acuacaagaa ccuggggaag     3180

aucuucgaca aggacggcuc ccagaaggag aucuacgaga cgcucgugaa guggcuggag     3240

gccaagagca aggacuaccc gaagcugaag aacgggaaca ucaucaagaa ggucaagauc     3300

guggacggca acaaggacaa gcucaucaag cugggcaaca agcgcuacgu ggagaugggg     3360

cggaccaccg ucaagauccu ggugcucaag aaggaggcgg aggagggccu gaaguucgcc     3420

uccaucgggc gcuacaagua caaccugcuc aagagcgaga aggacgugaa caucuccauc     3480

uggaaggacg ccauccacuu cugcaagguc cgguacaaca agcugaagga gaacggcuac     3540

cgccugaucc acgagcucau ccccggggag accaucgagc uggagcugaa caagggcaac     3600

aucagcaagu gccucguggu gggcuucucc gggggcaaga ucgagaucag cuccgucauc     3660

ggggacgccc uggaccugau caacgacaag aucagcacgc ggaucaagga gcgcuacuac     3720

aucaccgugu ccaccaucaa gagcaucaag aagaucaaca agaacauccu cggcgacuga     3780


<210>  1748
<211>  3792
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1748
augcacaaga acaaccccga cauccgcgag cuguuccucg ccauccacca cauccugaag       60

uaccggggcc acuuccuguu cgccgggcag caguucaagg gcagcgagau caaggacaac      120

auccgcgagc uccugcagua caacucccug aacaucaaga ucgacaucac cgacgagcuc      180

aucaacaaca ucgcggagau ccugcugaag aagaagaaca gcaccgacaa gaucaucgag      240

uucaagaagc uguucgccga cgagaagcag cugaucgagg cccugcggcu cgcccugggg      300

aacaagacgc agcuggccaa gcuguucgac aucgaggcgu acaaggagcu ggacaucucc      360

aucaagacca ucagcuucaa ggagaagauc uacgaggacg ugcgcuccaa cuacgaggag      420

auccugggcg aguacaucau cguccucgac accgccaagc ugaucuacga cgccaucauc      480

cugagcgaga ucaagaucaa gaacaugacc aucuccgaga gcaaggugau cuccuacgag      540

aagcacggga aggaccucaa gcagcugaag cagcugauca aggaggacag caagcucucc      600

aagaaggaga agcggcagcu guucgagaac aucuucguga aggaggacga gaaggaggcc      660

aacuacguca acuacaucgg caagaacaac gugggcagcu ccaagagcuc cugcagcuac      720

gaggacuuca agaagucccu gaucaaggag cucaaguacu gcgaggagag caagcugaag      780

caggacaucc uggcggagcu cgagcuggag acguuccugc cgcgccagcg gaccaaggac      840

aacuccguga uccccuacca gauccacaag gaggagcucg agaagauccu gaacaacgcc      900

guccaguacc accccuuccu gaacaacacc gacgaguacg ggaucagcau caaggagaag      960

aucauccagc uccuggaguu ccgcaucccc uacuacgugg gcagccugaa caccucccac     1020

aaggugggga acggcggguu cagcuggucc guccggaaca agggcuacga gcgcacggcc     1080

gugaccccgu ggaacuucaa caaggugauc aacaaggagg agagcgccaa gaacuucauc     1140

gagaaccuca ccaaccagug caccuaccug cuguacgagg acguccuccc caagcacucc     1200

cugcuguaca gcgaguucac gcuccugaac gagcugaacu ccaucaacua cggcgggaag     1260

cggcucaccg uggagcaccg caaccugauc aucgagcggu ucuucaagca gagcgccccc     1320

aaggcgaccg gcaaggagcu gaagaaguuc cugauguccg agggggugau cgacggcaag     1380

gacgagcuca gcggcuucga caucuccauc aagaacgacc ucaagagcua ccgcgacuuc     1440

aagaagaucc ugggggagaa guucgacucc gagaaggucg agaagaugau ccgguggauc     1500

acccucuucg gcgagagcaa gcagcugcug guggacaaga ucacgaagga guacggggac     1560

aucuacucca gcgaggagau caagaagauc uccggccuca ccuacaagga cugggggcgc     1620

cugagcgaga agcugcucac cgagaucgug uccagccagc uguacgacga gagcaccggc     1680

gagugcuuca acaucaucaa cgccaugcgg aacaacacgc uccucuucau cgagcugcug     1740

ggcaccaacu acgacuacuu cgagaagauc aacaccuaca acgggaagcu ccgcaaggag     1800

aucaccgaga ucacgcccca cauccuggac gaccuguacg ucuccccggc cgugaagcgg     1860

agcaucuggc aggucgugcg caucguggag gagcucaaga aggugaucgg cuccgagccc     1920

aagcggaucu ucgucgagac cacccgcagc aacaaggaga agaaggggga cgacggccgc     1980

aaggacuccc ggaagaaggc ccugcuggag cucuacaaga agaucaagac cagcgacauc     2040

gccgagcugg agaaggcgcu ggggcacacg guggacuucg acaagaagaa gcaggaccuc     2100

accgacaucg acaacaacaa gcugaaggcc cgcaagcugu accucuacua cacccagcug     2160

ggccggugcc uguacaccgg caaggccauc gaccucaaca accuguucgg guccaacagc     2220

gaguacgaca ucgaccacau cuacccccag uccaagacga aggacggcag cuggaacaac     2280

cugguccucg ugaacaagac cgagaacucc aagaagucca acgggcugau cagcgagagc     2340

auccagcgca agaacaagca cuucuggcgg uuccugaagg ccaagaaccu caucuccgag     2400

gagaaguaca agcgccugac ccggaccgag gacuucacgg acggcgagcu gagcggguuc     2460

aucgcccgcc agcucaucga gaccucccag agcgugaagg ucgugagcaa gauccugcag     2520

aaccugaacc ccaccaccga gcucugcuuc uccaaggcgg agaacgugag cgacuuccgg     2580

caggaguucg gcaagaucaa ggucggcaag cgcaagaacc ugaccgagga ggagcagaag     2640

aaggccaagg aggggcagcu gaucaaggug cgggaccuca acgaccugca ccacgccaag     2700

gacgccuacc ugaacaucgu ggucggcaac guguaccaca ucaaguucac gcgcaacccg     2760

aagaacuuca uccggaagaa cuucaagaac cgcgaguaca cccucaacac ccuguucuac     2820

uccgacaagg uggagcggaa cggggagguc gccugggaga agggcaagac cgaggagauc     2880

gugagcuccg ugaugugcaa caaccugauc aacgucacgc gccggaccag cgagcagaag     2940

ggcgcgcucu acgaggccac caucuacaag gccaacgccg cgaaggaggg guccuacuac     3000

cccgugaaga ccaacaacaa gaaccugaag aacgugacgc gcuacggcgg guucaccagc     3060

aucaagaucg ccuacuacuc caucuucaag uacgacaucc ggaacaagga caaggucaag     3120

gagaacauca agaucauccc cauccccauc uacaucagcc agaacaucaa gaccgaccug     3180

gaccucauca acuucgccga gaagcagauc aagcugaaga agggcgagaa caucgagaac     3240

cugcagcuca ucuacaagaa gcugaacauc ggggcccugg ugaagaucga ccacuacccg     3300

uacuacaucg gcggcaagac cgacgacaac uucuacgugg acggggccgu ccaggugcuc     3360

cuguccagcg acaucgagcg cuaccugaag gaggugguca aguucgugaa cuuccagcgc     3420

gagaacaagg aggcgaaggu guccaaggag cacaucagcc ucgaggagaa caucaagcug     3480

uacaacacgc uggucugcaa gauggccacc aagaucuuca cccacaagaa gaacaacaag     3540

uacaacgacc ucacccagga caaggccaag aacacguuca acaagcuguc ccuggaggag     3600

cagugcaagg ugcuccugga gauccugaac cuccugacca acaagaagac caccuucgac     3660

cugagccuca ucaacgagaa gacgucccgg gugaagugcg gcuucaaccu gcugaaccag     3720

aagcaguuca gcaucaucga gcaguccguc accgggcucu acgagaagga gaucaagauc     3780

aucgaggacu ga                                                         3792


<210>  1749
<211>  3795
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1749
augaagaacg ugcugggccu cgaccugggg accaacagca ucggcugggc ccugaucgag       60

gucgacgaga acaaccuccc cauccgcauc aucgccaugg ggucccggau caucccgcug      120

agcaccuccg acaaggagga guuccagcgc ggccugagca ucacgaagaa ccagucccgg      180

accgugagcc gcacccagcg gaagggguac gaccgcaagc agcucaagaa guccgaccug      240

aagaagaucc ugaacaagua caacaucuuc cccagcgagg agcuccugcg gcugcccaug      300

cucgaccugu ggaagcugcg cuccgacagc gcgaaccccg acgagaacau cuccgccgag      360

cagcucggcc ggauccugua caugcugaac cagaagcgcg gcuacaagag cgcccggucc      420

gaggccaacg ccgacaagaa ggacagcgac uacgugcagg cggucaaggg gcgcuacgac      480

cagcucaagg accggaagca gaccaucggc caguacuucu acgacgagcu guacaacgcc      540

aacgccaaca acuccuacuu ccgcaucaag gaggaggugu acccgcggga ggccuacauc      600

gaggaguucg acacgaucau cagcgcgcag cgcgagaagc acgacuuccu gaccgacgag      660

gugguccacc agcuccggaa cgagaucauc uacuuccagc gcaagcugaa gucccagaag      720

gggcugguga gcaucugcga guucgagggc uucgagaaga ccguguucga caaggagaag      780

aacaagaaca aggucguguu cguggggccc aaggucgccc ccaagaccag cccccucuuc      840

cagcugugcc ggaucuggga gguggugaac acgaucaccc ugaagaucaa gaacccggag      900

ggcuccaagu acaagugggg cgagaagauc cccgacauca aggagaagga gauccucgcc      960

gacuaccugu ucaagaacga gagccugucc uucagcaagc uccuggagau ccuggagcuc     1020

aagaaggaga acgucuacgu gaacaagcag auccugaagg ggaucaaggg caacgagacc     1080

uacuccgccc ugcacagcau ccucggggac aacgagcugc ugaacuucga cguguccauc     1140

augaccagca accagacggc ccuccugguc gaccgcaaga ccggcgagau ccuggaggag     1200

cgggcgggcc ucgagcugga cgccucccug gagaaggagc cccucuacca gcuguggcac     1260

accaucuaca gccugaagga ccucgacgag ugcaagaacg cccugaucaa gcgcuucgac     1320

uucgacgacg agaccgccga gaagcugucc cgcaucgacu ucaacaagca ggccuucggg     1380

aacaagagca acaaguccau gcggaagauc cugcccuucc ucauggaggg cuacgacuac     1440

agccagucca gcucccuggc gggguacaac cacagcaacu cccugacgaa gaacgagcgc     1500

gagcagcaga ucacccucga ccggcuggag cugcucccga agaacagccu gcgccagccc     1560

aucguggaga agauccugaa ccagaugauc aacgugguca acgccauccu cgacgaguac     1620

ggcaagcccu ccgagauccg gguggagcug gcccgcgagc ugaagcagag caaggacgag     1680

cggaacgaga ccgaccucca gaacagcaag aacaagaagc ugaacgacga gaucgggaag     1740

cgccuggagg agcucggccu gcccgugacc aagcgguaca uccagaagua caaguucauc     1800

uucccguccc ugagcaagaa ccucaaggag gccaagguca acaaccagug caucuacugc     1860

ggcgagacgu ucaaccugac cgaggcccug uccggggacg cguacgacgu ggaccacauc     1920

guccccaagg cccuccuguu cgacgacagc cagaccaaca aggugcuggu gcaccgcaag     1980

ugcaacucca ccaagacgaa ccagaccgcc uacgacuaca ucgccaccaa gggcgacgag     2040

caccucaagc uguacagcga gcggcuggac gacugguucg agcgcgggau caucuccuac     2100

agcaagaugc agcggcucaa ggucucccac aaggaguacc uggagcgcaa gaagcugggc     2160

aaggagaccg agacggacaa gaagcucugg gagaacuuca ucgaccggca gcugcgcgac     2220

acccaguaca ucagccggaa gucccgcgag auccugcaga agaucugcaa cgacgugaag     2280

accaccgagg gcggggugac ggccaagcuc cggaaccugu ggggcuggga cgacguccug     2340

augaaccucc agaugcccaa guacaaggag cuggggcaga ccaucaccaa ggaguggacc     2400

agcgagcacg gcaagcgcaa gcaccagaag gaggagaucg acaacuggac gaagcgcgac     2460

gaccaccggc accacgcgau cgacgcccug gugaucgccu gcaccaagca gggguucauc     2520

cagcgcauca acacccucaa cuccagcgac accaaggacc ugaugaucaa ggagguggag     2580

gaggccaaga ucgaguucaa cgagaagacc acgcugcucg agaaguaccu gaagacccag     2640

aagcccuucu ccacccagga ggucaugaag gaggcggaca agauccuggu gagcuucaag     2700

gccggcaaga agguggccac caucacgaag uucaaggcca ccgggaagaa caaggagacc     2760

ggcgucaucg ugccgcgggg cgcccuccac gagcaguccg uguacgggaa gaucaagguc     2820

aucgagaagg acaagccccu gaaguaccug cucgagaaca gcgacaagau cgugaacccc     2880

cgcaucaaga accugaucga gaucaagcug agcgagaaca agaacgaccc caagaaggcg     2940

cuccuggccc ugaagaagau cccgaucuuc cucgacgagg agcaguccau cacccuggag     3000

aaggccagcu gcuacaacga cgccacggug cugaaguaca agcuccagaa ccugaaggcc     3060

gcgcaggucg acgacaucgu ggacgagaag gugaagaaga ucguccggga gcgccugucc     3120

caguucggca acaaggagaa ggaggccuuc aaggacaccc ucugguucaa cgaggagaag     3180

aagaucccca ucaucaccgu gcggcuguuc gcccgccccg acgccaacaa ccugcagacc     3240

aucaagaagg acgagaacgg gaacggcauc ggcuucgugg ucacggggaa caaccaccac     3300

aucgccaucu acgaggacaa ggacaacaag cccgugcagc acagcugcac cuucuggcac     3360

gcgguggagc ggaagaagua caacaucccg gccgucaucc gcgacuccag cgaggugugg     3420

aacgaccucc ugcggaagga gcugcccgag uccuuccuca acaagcugcc cgccgacaac     3480

cugaaccuca aguucagcau gcagcagaac gagauguuca uccugggccu gucccaggac     3540

gaguucguga aggccaucgc gaacaacgac aagagccucc uguccaagca ccuguaccuc     3600

gucuggagca ucuccgagaa caacuacugg uuccgccacc accuggagac caagaacagc     3660

gaccugaaga agaccgacgg ggugaaggag uccaagcggc ucuacaacau ccgcagccug     3720

ggcucccugu ucagccucaa cccgaucaag gugcggcuga acuaccuggg ggagaucacg     3780

aagaucggcg aguga                                                      3795


<210>  1750
<211>  3801
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1750
auggccaagu acauccuggg ccucgaccug gggaccaaca gcaucggcug ggcccugauc       60

gagguggacg agaacaacau ccccauccgc aucaucguca uggggucccg gaucaucccg      120

cucagcucca acgaccgcga ggaguuccag cggggccuga gcaucaccaa gaaccagaac      180

cgcacgaucu cccggaccca gcgcaagggg uacgaccgga agcagcugaa gcgcaacgac      240

cucaacaagg ugcugaacaa guacaacauc uuccccagca agaugcugcu caaccugccc      300

augcuggacc ucuggaagcu gcgguccgac agcgcgaacu ccaacaagga caucagcgcc      360

gagcagcugg gccgcauccu cuacaugcug aaccagaagc ggggcuacaa guccgugcgc      420

agcgaggcca acuccgacaa gaaggacacc gacuacgucc aggccgugaa ggggcgguac      480

gcccagcuga aggaccgcaa ccagaccauc ggccaguacu ucuacgagga gcucuacaac      540

gcgaacguga acaacagcua cuucaagauc aaggaggagg ucuacccccg ggaggccuac      600

aucgaggagu ucgacacgau caucaacucc cagaagacca agcacgaguu ccugaccgac      660

caguucgugc accagcugcg caacgagauc aucuacuucc agcggaagcu caagagccag      720

aaggggcuga ucuccaucug cgaguucgag ggcuucgaga agaccguguu cgacaaggag      780

aagaacaagc acaagaucgu cuucgugggg ccgaaggugg cgccccgcag cgcgccccug      840

uuccagcucu gccgggucug ggaggugauc aacacgaucu ccaucaaggu gaagaacccc      900

gagggcagca aguacaagug gggcgagcgc aucccgagca ucaaggagaa ggagauccug      960

gccgacuacc uguucaagaa cgagguccuc uccuucacca agcugcugga gauccucggg     1020

cugaacaaga acgacgugua cgugaacaag cagauccuga agggcaucaa ggggaacgag     1080

accuacgcca cccucuacaa cauccugggc gagaacgagu uccugaacuu cgacgucagc     1140

aucauccccu ccaaccacac gagcaugcuc guggacaaga agaccggcga gauccuggag     1200

gagcgggccg ggcuggagcu cgacgugucc cuggagaagc agcccuucua ccagcugugg     1260

cacaccaucu acagccucaa ggaccuggac gagugcaaga acgcccugau caagcgcuuc     1320

gacuucgacg acgagaccgc cgagaaccuc ucccgcaucg acuucaacaa gcaggccuuc     1380

ggcaacaaga gcaacaaguc caugcggaag auccugcccu uccugaugga gggguacgac     1440

uacagccagu ccugcagccu cgccggcuac aaccacucca acagccugac gaaggacgag     1500

cgcgagcagc aggucacccu ggaccggcug gagcuccugc cgaagaacuc ccugcgccag     1560

cccaucgugg agaagauccu caaccagaug aucaaccugg ugaacgccau ccuggaccag     1620

uacggggagc ucagcgagau ccgggucgag cuggcccgcg agcugaagca guccaaggac     1680

gagcggaacg acgccgaccu ccagaacacc aagaacaaga agcugaacga cgagaucggc     1740

aagcgccugg cggagcucgg ccugcccauc accaagcggu acauccagaa guacaaguuc     1800

aucuucccca gccuguccaa gaaccucaag gacgcccacg ugaacaacca gugcaucuac     1860

ugcggggaga cguucaaccu gagcgaggcc cugagcggcg acgccuacga cgucgaccac     1920

aucgugccga aggcccuccu guucgacgac ucccagacca acaaggugcu gguccaccgc     1980

aagugcaaca gcaccaagac caaccagacg gcguacgacu acaucuccac caagggggac     2040

gagcaggucc ggaucuacuu cgagcgcgug gacgacuggu ucaagagcgg caucaucucc     2100

uacagcaaga ugcagcggcu caaggugucc cacaaggagu accuggagcg caagaagauc     2160

gggaaggaga ccgagaccga caagaagcug ugggagaacu ucaucgaccg gcagcuccgc     2220

gacacgcagu acaucagccg gaagucccgc gagauccugc agaaggugug caacaacgug     2280

accaccaccg agggcggcgu cacggccaag cugcggaacc ucugggggug ggacgacgug     2340

cugaugaacc ugcagaugcc caaguaccgc gagcucggcc agaccgugau caaggagugg     2400

accagcgagc acgggaagcg caagcaccag aaggaggagg ucgagaacug gaccaagcgg     2460

gacgaccacc gccaccacgc caucgacgcc cuggugaucg ccugcacgaa gcagggcuuc     2520

auccagcgga ucaacacccu gaacuccagc gacaccaagg accucaugcu gaaggaggug     2580

gaggaggcga aggucaaguu cuccgagaag accacccugc ucgagaagua ccugcgcacg     2640

caggagcccu ucagcaccca ggaggugaug cgggaggccg acaaggugcu ggucuccuuc     2700

aaggccggga agaagguggc caccaucacc aaguucaagg cgaucggcaa gaacaaggac     2760

agcggcguga ucgucccccg cggggcccuc cacgagcagu ccguguacgg caagaucaag     2820

gugaucgaga aggacaagcc gcugaaguac cuguucgaga acagcgaccg gaucgucgac     2880

cccaagguga agaagcucgu ggagguccgc cugagcgaga acgagaacaa cuccaagacg     2940

gccaucagca cccugaagaa ggcccccauc uuccucaacg aggagaagac cgaggugcug     3000

gagaaggccu ccugcuacaa cgacgcgacc gugcugaagu acaagcucca gagcaucaag     3060

gccucccagg ccgacgacau cgucgacgag cgggugaagu uccuggugaa ggagcgccug     3120

gcccaguucg ggaacaagga gaaggaggcc uucaaggaca uccucugguu caacgaggag     3180

aagcagaucc ccaucaucag cguccggcug uucgcgcgcc cggacgccaa caaccuccag     3240

gugaucaaga aggacgagaa cggcaagucc aucggcuucg ugcugacggg gaacaaccac     3300

cacaucgcca ucuacgagga caaggacaac aagcugaucc agcacagcug caccuucugg     3360

cacgccgucg agcggaagaa guacaacauc cccgugguga ucaagaacuc cagcgacguc     3420

uggucccagc ugcucgacaa gcagcugccc gagagccugc ucaacaagcu gcccaccgac     3480

aagcugaacc ucaaguucuc caugcagcag aacgagaugu ucauccuggg ccugagccag     3540

aaggaguucg acgaggccau caagaacaac cacaaguccu uccucagcaa guaccuguac     3600

cugguguggu ccaucagcaa caacgacuac uacuuccgcc accaccucga gaccaagaac     3660

acggagcuga agaacaucuc caccgcgaag gagagcaagc gguacuuccg cuucaagagc     3720

gugggggccc uggucggcac caacccgauc aagauccggc ucaaccaccu gggggagauc     3780

aacaaggugg gcgaguacug a                                               3801


<210>  1751
<211>  3807
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1751
augaccaaga acauccuggg ccucgaccug gggaccaaca gcaucggcug ggcccugauc       60

gagguggacg agaacaacac gcccauccgc aucaucgcca uggggucccg gaucaucccg      120

cucagcaccu ccgacaagga ggaguuccag cgcggccuga gcaucaccaa gaaccagucc      180

cggaccguca gccgcacgca gcggaagggg uacgaccgca agcagcugaa gaaguccgac      240

cucaagaaga uccugaacaa guacaacauc uuccccagcg aggagcugcu caagcugccc      300

augcuggacc ucuggaagcu gcgguccgac agcgcgaacc ccaacgagaa caucuccgcc      360

gagcagcugg gccgcauccu cuacaugcug aaccagaagc ggggcuacaa gagcgcccgc      420

uccgaggcca acgccgacaa gaaggacacc gacuacgugc aggcggugaa ggggcgguac      480

gcccagcuga aggagcgcaa ccagaccauc ggccaguacu ucuacgagga gcucuacaac      540

gccaacacca acaacagcua cuuccggauc aaggaggagg ucuacccgcg cgaggccuac      600

acggaggagu ucgacaccau caucaacgcg cagaagaaca agcacgacuu ccugaccgac      660

gagguggugc accagcugcg gaacgagauc aucuacuucc agcgcaagcu caagucccag      720

aaggggcugg ucagcaucug cgaguucgag ggcuucgaga agaccguguu cgacaaggag      780

aagaacaagg acaagguggu cuucgugggg cccaaggugg cccccaagac gagcccccug      840

uuccagcucu gccggaucug ggaggucgug aacaccaucu cccugaagau caagaacccg      900

gagggcucca aguacaagug gggcgagaag auccccaacc ugaaggagaa gcagauccuc      960

gccgacuacc uguucaagaa cgacagccug agcuucacca agcuccugga gauccuggac     1020

cucaacaaga acgacgugua cgucaacaag cagauccuga aggggaucaa gggcaacgag     1080

accuucucca cgcugcacac cgugcucggg gacaacgagc ugcugaacuu cgacgugagc     1140

aucauccccu ccaaccacac cagcaugcuc gucgacaaga agaccggcga gauccuggag     1200

gagcgcgccg gccuggagcu cgacgccucc cuggagaagg agccccugua ccagcucugg     1260

cacacgaucu acagccugaa ggaccuggac gagugcaaga acgcgcucau caagcgguuc     1320

ggguucgacg acgagaccgc cgagaagcug ucccacaucg acuucaacaa gcaggccuuc     1380

ggcaacaaga gcaacaaguc caugcgcaag auccugccgu uccugaugga gggguacgac     1440

uacagccagu ccugcagccu cgccggcuac aaccacucca acagccugac caaggacgag     1500

cgggagcagc agaucacccu ggaccgccuc gagcugcugu ccaagaacag ccuccgccag     1560

cccaucgugg agaagauccu gaaccagaug aucaacgugg ucaacgccau ccuggagcag     1620

uacgggaagc ucuccgagau ccggguggag cuggcgcgcg agcugaagca gagcaaggac     1680

gagcggaacg acgccgaccu ccagaacucc aagaacaaga agcugaacga ggagaucggc     1740

aagcgccugg ccgagcucgg ccugcccgug acgaagcggu acauccagaa guacaaguuc     1800

aucuucccca gccugagcaa gaaccucaag gacgcccacg ucaacaacca gugcaucuac     1860

ugcggggaga ccuucaaccu gaccgaggcc cuguccggcg acgcguucga cguggaccac     1920

aucgucccga aggcccuccu guucgacgac agccagacca acaaggugcu ggugcaccgc     1980

aagugcaacu ccacgaagac caaccagacc gccuacgacu acaucgggac caagggcgag     2040

gagcaggucc ggguguacag cgagcgcauc gacgacuggu ucaagcgggg gaucaucucc     2100

uacagcaaga ugcagcgccu caaggugucc cacaaggagu accuggagcg gaagaagcug     2160

ggcaaggaga cggagaccga caagaagcuc ugggagaacu ucaucgaccg ccagcugcgg     2220

gacacccagu acaucagccg caaggcccgg gagauccugc agaaggucug caacaacgug     2280

accacgaccg agggcggggu gaccgccaag cuccgcaacc uguggggcug ggacgacguc     2340

cugaugaacc uccagaugcc caaguacaag gagcuggggc agaccgugau caaggagugg     2400

accuccgagc acggcaagcg gaagcaccag aaggaggaga ucgagaacug gacgaagcgc     2460

gacgaccacc ggcaccacgc gaucgacgcc cugguggucg ccugcaccaa gcagggguuc     2520

auccagcgca ucaacacccu caacagcucc gacaccaagg accugaugca gcgcgagauc     2580

aaggaggcca agaagcugcu cggcgagaac uacaacgaga aggaguucga gaacgacaac     2640

gaguucguga agaaccacaa ggagcggcug agccagcugg acgacuaccu ccgccugcag     2700

aagcccguga aguucgacac gaaguacguc gagcaggagg cggacaaggu gcuggugucc     2760

uucaaggccg gcaagaaggu cgccaccauc accaaguuca aggccaccgg gaagaacaag     2820

gacacgggcg ugaucgugcc ccgcggggcc cuccacgagg cgggccugua cggcauccgg     2880

accaucuaca gcaagggcgg gaucgccgag aagcacauca ucaagaagua caaggucggg     2940

guguccgccc aggaccacau cuucaccggc aaggagaccu acagcgugaa gaaggacaag     3000

aacgggaacg agaaggucga cgacaagauc aagaucgugc uggacuccau cguggacaac     3060

aaggucaagg agguggugcu caaccggcug aaccgcggcu ucaacaacga ggaggacuac     3120

cggaucgacg ucaagaaggc ccugaacaac uucaagaacc ucgacgagga cccgcugugg     3180

uacaacgaga gcaagggcau caagauccug agcaucaagc ucaagacggg gcugaaggac     3240

uccguggugc cccugcgccu caacgagaag aacgagguca ucacccuggu gaagaccggc     3300

aacaaccacc acaucgccau cuacgaggac aacgacggga agcugaucca guacagcugc     3360

accuucuggc acgcggugga gcggaagaag uacaacaucc ccacggucau caagaacucc     3420

agcgaggugu ggaaccaggu gcucgacaag aagcugcccg aguccuuccu gaaccgccuc     3480

ccggccgaca agcugaaccu gaaguucagc augcagcaga acgagauguu cauccucggc     3540

cugcuggagg aggaguucga caaggccauc aaggacaacg acaagucccu ccugagcaag     3600

uaccuguacc ucgucugguc caucgccgag ggggacuacu gguuccggca ccaccuggag     3660

accaagaaca ccgagcugaa gaagaccaac ggcgccaagg agagcaagcg cuacuuccgg     3720

cucuccacga agggccugau cgaccugaac cccgugaagg ugcgccucaa ccaccugggg     3780

gagaucacca agaucggcga guacuga                                         3807


<210>  1752
<211>  3810
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1752
augaacaaca gcaucaaguc caagcccgag gugaccaucg gccuggaccu cggggucggc       60

agcguggggu gggccaucgu ggacaacgag accaacauca uccaccaccu gggcucccgc      120

cuguucagcc aggccaagac ggcggaggac cggcgcuccu uccgcggggc ccggcggcuc      180

auccgccggc gcaaguacaa gcugaagcgg uucaucaacc ugaucuggaa guacaacagc      240

uacuucggcu ucaagaacaa ggaggacauc cucaacaacu accaggagca gcagaagcug      300

cacaacaccg uccugaaccu caagcuggag gcccugaacg ccaagaucga cccgaaggcc      360

cucuccugga uccugcacga cuaccugaag aaccgcggcc acuucuacga ggacaaccgg      420

gacuucaacg uguaccccac cgaggagcuc gcgaacuacu ucggggagua cggcuacuac      480

aaggggauca ucgacagcaa ggaggacaac gacgacaagc uggaggaggg ccugaccaag      540

uacaaguucu ccaaccagca cuggcucgag gagaucaaga aggugcugag caaccagacg      600

gggcugcccg agaaguucaa ggaggaguac gagucccucu ucagcuacgu ccgcaacuac      660

uccgagggcc ccggcagcau caacuccgug agcccguacg ggaucuacca ccuggacgag      720

aaggagggca agguggucca gaaguacaac aacaucuggg acaagaccau cgggaagugc      780

uccaucuucc ccgacgagua ccgggccccc aagaacagcc ccaucgccau gaucuucaac      840

gagaucaacg agcuguccac cauccgcagc uacagcaucu accucaccgg cugguucauc      900

aaccaggagu ucaagaaggc cuaccugaac aagcugcucg accugcugau caagacgaac      960

uccgagaagc cgaucgacgc gcggcaguuc aagaagcucc gcgaggagac caucgccgag     1020

agcaucggca aggagacccu gaaggacgug gagcgggagg agaagcugga gaaggaggac     1080

cacaagugga agcucaaggg gcugaagcug aacaccaacg gcaagaucca guacaacgac     1140

cucuccagcc uggccaaguu cgugcacaag cugaagcagc accucaagcu ggacuuccug     1200

cucgaggacc aguacacgac ccuggacaag aucaacuucc ugcagucccu cuucgucuac     1260

cuggggaagc accuccgcua cagcaaccgg guggacuccg ccaaccucaa ggaguucagc     1320

gacuccaaca agcuguucga gcgcgugcug caggagcaga aggacgaccu guucaagcug     1380

uucgagcaga ccgacaagga cgacgagaag auccuggccc agacccacag ccuguccacg     1440

aaggcgaugc uccuggccau cacccggaug accaaccugg acaacgacga ggacaaccag     1500

aagaacaacg acaagggcug gaacuucgag gccaucaaga acuucgacca gaaguucauc     1560

gacaucacca agaagaacaa caaccucagc cugaagcagg acaagcgcua ccuggacgac     1620

cgcuucauca acgacgccau ccucuccccc ggggucaagc ggauccugcg cgaggccacg     1680

aagguguuca acgcgauccu gaagcaguuc agcgaggagu acgacgugac caaggucgug     1740

aucgagcucg cccgggagcu guccgaggag aaggagcugg agaacaccaa gaacuacgag     1800

aagcucauca agaagaacag cgacaagauc uccgagcgcc uggaggcccu ggacaucgcc     1860

gaggacaaga ucgaggacau ccucaagagc cccaccaagu ccuacaaggu gcugcugugg     1920

cuccagcagg accacaucga ccccuacagc cagaaggaga ucgccuucga ggacauccug     1980

acgaagaccg agaagaccga gaucgaccac aucaucccgu acagcaucuc cuucgacgac     2040

agcuccagca acaagcugcu cguccuggcg gaguccaacc aggccaagag caaccagacc     2100

cccuacgagu ucaucacguc cggcaacgcc ggcaucaagu gggaggacua cgaggccuac     2160

ugccggaagu ucaaggacgg ggacaccagc cugcucgacu ccacccagcg cagcaagaag     2220

uucgccaaga ugaugaagac cgacacgucc agcaaguacg acaucggcuu ccuggcgcgg     2280

aaccugaacg acacccgcua cgccaccauc guguuccggg acgcccucaa ggacuacgcc     2340

aacaaccacc ugguggagga caagcccaug uucaaggucg ugugcaucaa cgggggcguc     2400

accuccuucc ugcgcaagaa cuucgacaag agcugguacg cgaagaagga ccgggacaag     2460

aacauccacc acgccgugga cgccuccauc aucagcaucu ucuccaacga gacgaagacc     2520

cucuucaacc agcugaccca guucgccgac uacaagcugu ucaagaacac cgacgggagc     2580

uggaagaaga ucgaccccaa gaccggcgug gucacggagg ugaccgacga gaacuggaag     2640

cagauccgcg ugcggaacca ggucagcgag aucgccaagg ugaucaacaa gugcauccag     2700

gacuccaaca ucgagcgcaa ggcgcgguac agccgcaaga ucgagaacaa gaccaacauc     2760

ucccucuuca acgacaccgu guacagcgcc aagaaggucg gcuacgagga ccagaucaag     2820

cggaagaacc ugaagacgcu ggacauccac gaguccgccg aggagaacaa gaacagcaag     2880

gugaagaagc aguucgugua ccgcaagcuc gucaacgugu cccugcugaa caacgacaag     2940

cucgccgacc uguucgccga gaaggaggac auccugaugu accgggcgaa cccgugggug     3000

aucaaccucg ccgagcagau cuucaacgag uacaccgaga acaagaagau caagagccag     3060

aacgucuucg agaaguacau gcuggaccug accaaggagu uccccgagaa guucuccgag     3120

gccuucguca agagcaugcu ccgcaacaag accgccauca ucuacaacgu ggagaagaag     3180

guggugcacc gcaucaagcg gcugaagauc cuguccagcg agcucaagga gaacaagcug     3240

uccaacguga ucauccgcag caagaacgag uccgggacga agcugagcua ccaggacacc     3300

aucaacucca ucgcccucau gaucaugcgg agcaucgacc ccaccgcgaa gaagcaguac     3360

auccgcgucc cccugaacac ccugaaccuc caccugggcg accaggacuu cgaccugcac     3420

aacaucgacg ccuaccucaa gaagccgaag uucgugaagu accugaaggc caacgagauc     3480

ggggacgagu acaagcccug gcgggugcug acguccggca cccuccugau ccacaagaag     3540

gacaagaagc ugauguacau cagcagcuuc cagaaccuca aggacgucau cgagaucaag     3600

aaccugaucg agaccgagua caaggagaac gacgacuccg accccaagaa gaagaagaag     3660

gccaaccgcu uccugaugac ccucagcacg auccugaacg acuacauccu gcucgacgcg     3720

aaggacaacu ucgacauccu ggggcugucc aagaaccgga ucgacgagau ccucaacagc     3780

aagcugggcc uggacaagau caccaaguga                                      3810


<210>  1753
<211>  3810
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1753
augaacaaca gcaucaaguc caagcccgag gugaccaucg gccuggaccu cggggucggc       60

agcguggggu gggccaucgu ggacaacgag accaacauca uccaccaccu gggcucccgc      120

cuguucagcc aggccaagac ggcggaggac cggcgcuccu uccgcggggu ccggcggcuc      180

auccgccggc gcaaguacaa gcugaagcgg uucgugaacc ugaucuggaa guacaacagc      240

uacuucggcu ucaagaacaa ggaggacauc cucaacaacu accaggagca gcagaagcug      300

cacaacaccg ugcugaaccu caaguccgag gcccugaacg ccaagaucga cccgaaggcc      360

cugagcugga uccuccacga cuaccugaag aaccgcggcc acuucuacga ggacaaccgg      420

gacuucaacg ucuaccccac caaggagcug gccaaguacu ucgacaagua cggguacuac      480

aagggcauca ucgacuccaa ggaggacaac gacaacaagc ucgaggagga gcugaccaag      540

uacaaguuca gcaacaagca cuggcuggag gaggugaaga aggugcucuc caaccagacg      600

gggcugcccg agaaguucaa ggaggaguac gagagccugu ucuccuacgu ccgcaacuac      660

agcgagggcc ccggguccau caacagcgug uccccguacg gcaucuacca ccucgacgag      720

aaggagggca agguggucca gaaguacaac aacaucuggg acaagaccau cgggaagugc      780

aacaucuucc ccgacgagua ccgggcgccc aagaacagcc ccaucgccau gaucuucaac      840

gagaucaacg agcuguccac cauccgcagc uacagcaucu accugaccgg cugguucauc      900

aaccaggagu ucaagaaggc cuaccucaac aagcugcugg accuccugau caagacgaac      960

ggggagaagc cgaucgacgc ccggcaguuc aagaagcugc gcgaggagac caucgcggag     1020

uccaucggca aggagacccu caaggacgug gagaacgagg agaagcugga gaaggaggac     1080

cacaagugga agcugaaggg ccucaagcug aacaccaacg ggaagaucca guacaacgac     1140

cugagcuccc ucgccaaguu cgugcacaag cugaagcagc accugaagcu cgacuuccug     1200

cuggaggacc aguacgccac gcucgacaag aucaacuucc ugcagagccu guucgucuac     1260

cucggcaagc accuccggua caacaaccgc guggacuccg ccaaccugaa ggaguucagc     1320

gacuccaacc ggcuguucga gcgcgugcug caggagcaga aggacgggcu guucaagcug     1380

uucgagcaga ccgacaagga cgacgagaag auccucgccc agacccacag ccuguccacc     1440

aaggcgaugc ugcucgccau cacgcggaug accaaccugg acaacgacga ggacaaccag     1500

aagaacaacg acaagggcug gaacuucgag gccaucaaga acuucgacca gaaguucauc     1560

gacaucacca agaccaacaa caaccugagc cucaagcagg acaagcgcua ccuggacgac     1620

cgcuucauca acgacgccau ccuguccccc ggggucaagc ggauccuccg cgaggccacg     1680

aagguguuca acgcgauccu gaagcaguuc agccaggagu acgacgugac caaggucgug     1740

aucgagcugg cccgggagcu cuccgaggag aaggagcugg agaacaacaa gaacuacaag     1800

aagcugauca agaagaacag cgacaagauc uccgagggcc ucaaggcccu ggacaucgcc     1860

gaggacaaga ucgaggacau ccucaagagc cccaccaagu ccuacaaggu gcugcugugg     1920

cugcagcagg accacaucga ccccuacagc cagaaggaga ucgccuucga ggacauccuc     1980

accaagacgg agaagaccga gaucgaccac aucaucccgu acuccaucag cuucgacgac     2040

agcuccagca acaagcugcu gguccucgcg gaguccaacc aggccaagag caaccagacc     2100

cccuacgagu ucaucaccuc cggcaacgcc gggaucaagu gggaggacua cgaggccuac     2160

ugccgcaagu ucaaggacgg cgacacgagc cugcuggacu ccacccagcg gagcaagaag     2220

uucgccaaga ugaugaagac cgacaccucc agcaaguacg acaucggguu ccucgcgcgc     2280

aaccugaacg acacgcggua cgccaccauc guguuccgcg acgcccugaa ggacuacgcc     2340

aacaaccacc ucgucgagga caagcccaug uucaaggugg ugugcaucaa cggcgggguc     2400

accuccuucc ugcggaagaa cuucgacaag agcugguacg cgaagaagga ccgcgacaag     2460

aacauccacc acgccgugga cgccuccauc aucagcaucu ucuccaacaa gaccaagacg     2520

cuguucgacc agcucaccca guucgccgac uacaagcugu ucaagaacac cgacggcagc     2580

uggaagaaga ucgaccccaa gaccggcgug gucaccgagg ugacggacga gaacuggaag     2640

cagauccggg ugcgcaacca ggucuccaag aucgccgagg agaucgacaa gugcauccag     2700

gacagcaaca ucgagcggaa ggcgcgcuac agccggaaga ucgagaacaa gaccaacauc     2760

ucccuguuca acgacaccgu guacagcgcc aagaaggugg gguacgacga ccagaucaag     2820

cgcaagaacc ucaagacccu ggacaucgac gaguccgucg aggagaacaa gaacagcaag     2880

gugaagaagc aguucgugua ccggaagcug gucaacgugu cccuccugaa caacgacaag     2940

cuggccgacc ucuucgccga gaaggaggac auccugaugu accgcgccaa cccgugggug     3000

aucaaccugg cggagcagau cuucaacgag uacacggaga accggaagau caagagccag     3060

aacgucuucg gcaaguacau gcucgaccug accaaggagu uccccgagaa guucuccgag     3120

gccuucguca agagcaugcu gcgcaacaag accgccauca ucuacaacgu ggagaagaag     3180

guggugcacc gcaucaagcg gcucaagauc cuguccagcg agcugaagga gaacaagcuc     3240

uccaacguga ucauccgcag caagaacgag uccgggacca agcugagcua ccaggacacg     3300

aucaacuccg ucgcccugau gaucaugcgg agcaucgacc ccaccgccaa gaagcaguac     3360

auccgcgugc cccucaacac ccugaaccug caccucggcg accacgacuu cgaccugcac     3420

aacaucgacg cguaccugaa gaagccgaag uucgugaagu accucaaggc caacgagauc     3480

ggggacgagu acaagcccug gcggguccug aucuccggca gccugcucau ccacaagcgc     3540

gacaagaagc ugauguacau cuccagcuuc cagaaccuga acgaccucau cgagaucaag     3600

aaccugaucg agaccgagua caaggagaac guggacagcg accccaagaa gaagaagaag     3660

gccucccaga uccugcggag ccucuccacg auccugaacg acuacauccu gcucgacgcc     3720

aaggacaacu ucgacauccu gggccugagc aagaaccgca ucgacgagau ccucaacucc     3780

aagcuggacc uggacaagau cgcgaaguga                                      3810


<210>  1754
<211>  3822
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1754
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucgag       60

guggacgaga acaacuuccc cauccgcauc aucgccaugg ggucccggau caucccgcuc      120

agcuccaacg acaaggacga guuccagcgc ggccugagca ucaccaagaa ccagucccgg      180

acggucagcc gcacccagcg gaagggguac gaccgcaagc agcugaagaa guccgacgac      240

uucaaguaca gccuccggcg cauccugaac aaguacaaca ucuuccccuc cgaggagcug      300

cucaagcugc ccaugcugga ccucuggaag cugcggagcg acgcggugaa ccccaacgag      360

aacaucuccg ccgagcagcu gggccgcauc cucuacaugc ugaaccagaa gcggggcuac      420

aagagcgccc gcuccgaggc caacgccgac aagaaggaca ccgacuacgu gcuggcgguc      480

aaggggcggu acgcccagcu caaggaccgc aaccagacca ucggccagua cuucuacgag      540

gagcugcaca acgccaagac gaccaacagc uacuuccgga ccaaggagga gguguacccg      600

cgcgaggccu acaucgagga guucgacacc aucaucaacg cgcagaaguc caagcacgac      660

uuccugacgg acgagguggu ccaccagcuc cggaacgaga ucaucuacuu ccagcgcaag      720

cucaagagcc agaaggggcu gguguccauc ugcgaguucg agggcuucga gaagaccauc      780

uucgacaagg agaaggacaa gaacaaggug gucuucgugg ggcccaaggu ggcccccaag      840

accagccccc uguuccagcu gugccggauc ugggaggucg ugaacaccau cacgcugaag      900

aucaagaacc cggagggcuc caaguacaag uggggcgaga agauccccaa caucaaggag      960

aaggaggccc ucgccgacua ccuguucaag aacgagaccc ugagcuucaa caagcuccug     1020

gacauccugg agcucaagaa ggagaacgug uacgucaaca agcagaucau caaggggauc     1080

aagggcaacg agaccuacag cgcccugcac cgcauccugg gggacaacga gcuccugaag     1140

uucgacgugu ccaucauccc cagcaaccac accuccaugc ugguggacaa gaagacgggc     1200

gagauccucg aggagcgggc gggccuggag cuggacguca gccucgagaa ggagccccug     1260

uaccagcugu ggcacaccau cuacuccauc aaggaccucg acgagugcaa gaacgcccug     1320

aucaagcgcu ucggguucga cgacgagauc gccgagaagc ugagccaccu cgacuucaac     1380

aagcaggccu ucggcaacaa guccaacaag agcaugcgca agauccugcc guuccugaug     1440

gagggguaca acuacuccca gagcugcacc cuggccggcu acaaccacuc caacagccuc     1500

accaaggacg agcgggagca gcaggucacg cuggacaagc uggagcuccu gcccaagaac     1560

ucccugcgcc agcccaucgu ggagaagauc cucaaccaga ugaucaacgu ggugaacgcg     1620

auccuggagg aguacgggaa gcccagcgag auccggaucg agcuggcccg cgagcucaag     1680

caguccaagg acgagcggca cgacgccgac cugcagaaca gcaagaacaa gaagcugaac     1740

gaggagaucg gcaagcgccu caccgagcug ggccugccgg ugaccaagcg guacauccag     1800

aaguacaagu ucaucuuccc cucccucagc aagucccuga aggacgccca ggucaacaac     1860

cagugcaucu acugcgggga gaccuucaac cugacggagg cccucagcgg cgacgcguac     1920

gacguggacc acaucguccc caagagccug cuguucgacg acucccagac caacaaggug     1980

cucgugcacc gcaagugcaa cagcaccaag accaaccaga cggccuacga cuacaucgcc     2040

uccaaggggg aggaggaggu ccagaucuac agcgcccggg uggacgacug guucaagcgc     2100

ggcaucaucu ccuacagcaa gaugcagcgg cugaaggugu cccacaagga guaccuggag     2160

cgcaagagcc ucaacaagga gaccgagacc gacaagaagc ugugggagaa cuucaucgac     2220

cggcagcugc gcgacaccca guacaucucc cgcaagagca aggagauccu ccagaagguc     2280

ugcaacaacg ugacgaccac cgagggcggg gugaccgcca agcugcggga ccuguggggc     2340

ugggacgaca uccucaugaa ccugcagaug cccaaguaca aggagcugcg gcagacgguc     2400

aucaaggagu ggaccuccga gcacgggaag cgcaagcacc agaaggagga gaucgagaac     2460

uggaccaagc gggacgacca ccgccaccac gcgaucgacg cccucgugau cgccugcacc     2520

aagcagggcu ucauccagcg caucaacacc cugaacagcu ccgagacgaa ggaccugaug     2580

cagcaggaga ucaacgaggc caagaagcuc cugggggaga acuacaagga caaggaguuc     2640

gagaacagca acgagcuggu gaagaaccac aagggccggc ucuccaagcu ggacgacuac     2700

cugcgccucc agaagccgau caaguucaac accaaguacg ucgagaagga ggcggacaag     2760

auccugauca gcuucaaggc cgggaagaag guggccacca ucaccaaguu caaggccacg     2820

ggcaagaaca aggacaccgg cgugaucguc ccccgcgggg cccugcacga ggcgggccuc     2880

uacggggugc ggaccaucua cuccaagggc ggcaucgccg agaagcacau caucaagaag     2940

uacaaggugg gggucagcgc ccaggaccac aucuucaccg gcaaggagag cuacuccgug     3000

aagaaggaca agaacgggaa cgagaaggug gacgacaaga ucaaggucgu gcuggacagc     3060

aucguggacu ccaaggucaa ggagaucgug cugaaccggc ucaacuucgg cuucagcgac     3120

ggggaggacu acaagaacaa cgugaagaag gcccugaaca acuucaagaa ccuggacgag     3180

uacccccucu gguacaacga gaacaagggc aucaagaucc uguccaucaa gcugaagacg     3240

ggccucaagg acagcgucgu gccccugcgc cugaacgagu ccaacgaggu gaucacccuc     3300

gucaagaccg ggaacaacca ccacaucgcc aucuacgagg acaagaacag caagccggug     3360

cagcacuccu gcaccuucug gcacgcggug gagcggaaga aguacaacau ccccgccguc     3420

aucaagaagu ccagcgacgu guggaaccag cugcuggaca aggagcuccc cgagagcuuc     3480

cugaacaacc ugcccgagga caaccucaac cugaaguucu ccaugcagca gaacgagaug     3540

uucgugcugg gccucagcca ggaggaguuc aaggaggcca ucgagaacaa cgacaagucc     3600

cugcugagca aguaccucua ccuggucugg uccgugagcg agaacaacua cugguuccgc     3660

caccaccugg agacgaagaa cuccgaccuc aagaagaccg agggggccaa ggagagcaag     3720

cggcuguaca acauccgcag cguguccagc cuguucuccc ucaacccggu caaggugcgg     3780

cugaaccacc ugggcgagau caccaagauc ggggaguacu ga                        3822


<210>  1755
<211>  3825
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1755
augaccaagc acauccuggg ccucgaccug gggaccaaca gcaucggcug ggccaucauc       60

cagguggaca acaacaacaa cguccccauc cagaucaucg ccaugggguc ccgcaucauc      120

ccgcuggaca gcaacgaccg ggaccaguuc cagaagggcc aggcgaucuc caagaacaag      180

gaccgcacga ccgcccggac ccagcgcaag ggguacgacc ggaagcagcu caagaagucc      240

gacgacuuca aguacagccu gaagaagauc cuggagaagc ucgacaucuu ccccaccgag      300

gagcugauga agcugcccac gcucgaccug uggaagcugc gcagcgacgc cguguccaac      360

aucgaggaca ucacccccaa gcagcucggc cggauccugu acaugcugaa ccagaagcgc      420

ggcuacaaga gcgcccgguc cgaggccaac gcggacaaga aggacaccga cuacguggcc      480

gaggucaagg ggcgcuacac ccagcucaag gacaagggcc agacgcuggg gcaguacuuc      540

uacaaggagc ugagcgacgc caaccagaac aacaccuacu accgggugaa ggagaaggug      600

uacccgcgcg aggccuacau cgaggaguuc gacgcgauca ucaacgucca gaaguccaag      660

cacagcuucc ucaccgacga ggugauccac ucccugcgga acgagaucau cuacuaccag      720

cgcaagcuca agagccagaa gggccuggug uccaucugcg aguucgaggg guucgagacc      780

acguacuucg acaagaagac ccagcaggac aagaccaucu ucaccggccc caaggucgcg      840

ccccggacga gcccccuguu ccaguucugc aagaucuggg agguggugaa caacaucucc      900

cugaagacca agaacccgga gggcagcaag uacaaguggu ccgaccgcau ccccaccauc      960

gaggagaagc agaccaucgc caacuaccuc caggagaacg agaaccugag cuucaucgag     1020

cugcucaaga uccugcagcu gaagaaggag caggucuacg ccaacaagca gauccucaag     1080

gggauccagg gcaacacgac cuucagcgcc auccacaaga ucaucgggaa cuccgagcac     1140

cugaaguucg acaucgagac cauccccagc aagcacuucg ccgugcuggu ggacaagaag     1200

accggcgaga uccucgacga gcgggacucc cuggagcuga acagcgcccu cgagcaggag     1260

cccuucuacc agcuguggca cacgaucuac uccaucaagg accuggacga gugcaagaag     1320

gcccucauca agcgcuucaa cuucgaggag gagaucgccg agaagcugag caagaucgac     1380

uucaacaagc aggccuucgg caacaagucc aacaaggcga ugcggaagau gcugccguac     1440

cucaugcugg gguacaacca gagcgaggcc gaguccuucg ccggcuacaa ccgccggcug     1500

accaaggagg agaaguccaa gaacgucagc gacgagcccc uccagcugcu ggccaagaac     1560

agccuccgcc agcccguggu ggagaagauc cugaaccaga ugaucaacgu cgugaacgcc     1620

aucaucgaga aguacgggaa gcccgaggag auccgcgugg agcuggcgcg ggagcugaag     1680

caguccaagg acgagcgcga ggacgccgac aagcagaacg gcuucaacaa gaagcucaac     1740

gagcuggucg ccaccaagcu gaccgagcuc gggcugccga cgaccaagca cuacauccag     1800

aaguacaagu ucaucuuccc cgccaaggac aagaacugga aggaggccca gguggcgaac     1860

cagugcaucu acugcggcga caccuucaac cugaccgagg cccucagcgg cgacaacuuc     1920

gacguggacc acaucguccc caaggcccug cuguucgacg acucccaggc caacaaggug     1980

cucguccacc ggagcugcaa cuccacgaag accaacaaca ccgcguacga cuacaucacc     2040

aagaagggga gccaggcccu gaacgacuac guggcccgcg uggacgacug guucaagcgg     2100

ggcaucaucu ccuacgggaa gaugcagcgc cugaagguca gcuucgagga guaccaggag     2160

cggaagaaga ucggcaagga gacggaggcc gacaagcgca ucugggagaa cuucaucgac     2220

cggcagcucc gcgagaccgc cuacaucgcg aagaaggcca aggagauccu ggagaaggug     2280

ugccacaacg ugaccagcac cgaggggaac gucaccgcca agcugcggca gcucuggggc     2340

ugggacaacg ugcugaugaa ccugcagcuc cccaaguaca aggagcugga gaagaagacg     2400

aagcagaccu ucacccagcu gaaggagugg accuccgacc acggcaaccg caagcaccag     2460

aaggaggaga ucaucaacug gacgaagcgg gacgaccacc gccaccacgc caucgacgcc     2520

cucgugaucg cgugcaccca gcagggguuc auccagcgga ucaacacccu gagcuccagc     2580

gacgucaagg acgagaugaa gaaggagcug gaggaggaca agaccgugua caacgagcgc     2640

cucacgcugc uggagaacua ccuccuggag aagaagccgu ucuccaccga ggagaucgag     2700

aaggaggccg acaagauccu ggugagcuuc aaggccggca agaaggucgc cacccucucc     2760

aaguacaagg ccaccgggau caacgagauc aagggcgugc uggugccccg ggggccccug     2820

cacgagcaga gcgucuacgg caagaucaag gugaucgaga aggacaagcc ccucaaguac     2880

cuguucgaga acuccgacaa gaucgugaac ccgcugauca agcaccucgu caagacgcgc     2940

cugcuggaga acgagaacaa cgcgcaggcc gcccucguga cccugaagaa caagcccauc     3000

cugcucaaca acaagcagac cgagauccug gagaaggcca gcugcuacaa cgaggcgacc     3060

gugcugaagu acaagcucca gucccugaag gccagccaga ucgacgacau cgucgacgag     3120

aagaucaagu uccugaucaa ggagcggcuc uccaaguucg gcaacaagga gaaggaggcc     3180

uucaaggaca uccugugguu caacgagaag aagcagaucc ccaucacgag cauccgccug     3240

uucgcccgcc ccgacgccaa caaccuccag gugaucaaga agcacgagaa ggggaagaac     3300

aucggcuucg ugcuguccgg gaacaaccac cacaucgcga ucuacgagga caagaacaac     3360

aagcugaucc agcacaucug cgacuucugg cacgccgucg agcggaagcg caacaacauc     3420

ccggugcuca ucgaggacac cagcaccauc uggaaccacc ugaucaacga ggacuucucc     3480

gagagcuucc ugaacaagcu ccccaacgac agccugaagc ugaaguucuc ccuccagcag     3540

aacgagaugu ucauccuggg ccugcccaag gagcagagcg aggaggccau caaguccaac     3600

aacaagagcc uccuguccaa gcaccuguac cucgugugga gcaucaccga cggcgacuac     3660

uucuuccggc accaccugga gacgaagaac accgagcuga agaagaucga cggguccaag     3720

gagagcaagc gcuaccuccg gcuguccacc aagagccugg ucgaccucaa cccgaucaag     3780

gugcgccuga accaccuggg cgagaucacc aagaucgggg aguga                     3825


<210>  1756
<211>  3846
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1756
auggaggacg acagccagca cgacauccgc gagaucuacc uggccaucca ccacauggug       60

aaguaccggg gcaacuuccu cgucgagggg acccuggagu ccagcaacgc cuucaaggag      120

gacgagcugc ucaagcugcu gggccgcauc acccgguacg agauguccga gggggagcag      180

aacagcgaca ucgagcagga cgacgagaac aagcucgugg cgcccgccaa cggccagcug      240

gccgacgccc ugugcgccac gcgcgggucc cggagcaugc gcguggacaa cgcgcucgag      300

gcccuguccg ccgucaacga ccugagccgg gagcagcgcg ccaucgugaa ggcgaucuuc      360

gccggccucg agggcaacaa gcuggaccug gccaagaucu ucguguccaa ggaguucagc      420

uccgagaaca agaagauccu cgggaucuac uucaacaaga gcgacuacga ggagaagugc      480

guccagaucg uggacuccgg ccugcuggac gacgaggagc gggaguuccu cgaccgcaug      540

caggggcagu acaacgccau cgcccugaag cagcugcucg gccggagcac cuccgugagc      600

gacuccaagu gcgcgagcua cgacgcccac cgcgccaacu ggaaccugau caagcugcag      660

cuccggacca aggagaacga gaaggacauc aacgagaacu acgggauccu ggucggcugg      720

aagaucgacu ccggccagcg caagagcgug cgcggggaga gcgccuacga gaacaugcgg      780

aagaaggcca acguguucuu caagaagaug aucgagaccu ccgaccugag cgagacggac      840

aagaaccggc ucauccacga caucgaggag gacaagcugu ucccgaucca gcgcgacucc      900

gacaacggcg ucauccccca ccagcugcac cagaacgagc ucaagcagau caucaagaag      960

caggggaagu acuaccccuu ccugcuggac gcguucgaga aggacggcaa gcagaucaac     1020

aagaucgagg gccuccuggc cuuccgggug cccuacuucg uggggccgcu ggucgugccc     1080

gaggaccucc agaaguccga caacagcgag aaccacugga uggugcgcaa gaagaagggc     1140

gagaucaccc ccuggaacuu cgacgagaug gucgacaagg acgccagcgg gcggaaguuc     1200

aucgagcgcc uggugggcac cgacuccuac cugcucgggg agcccacccu gccgaagaac     1260

agccugcucu accaggagua cgaggugcug aacgagcuga acaacguccg ccucuccgug     1320

cggacgggca accacuggaa cgacaagcgc cggaugcgcc ugggccggga ggagaagacc     1380

cugcucugcc agcgccuguu caugaagggg cagaccguga ccaagcggac ggccgagaac     1440

cugcugcgca aggaguacgg ccggaccuac gagcucagcg ggcuguccga cgagagcaag     1500

uucaccucca gccuguccac cuacggcaag augugccgca ucuucgggga gaaguacguc     1560

aacgagcacc gggaccucau ggagaagauc guggagcugc agacggucuu cgaggacaag     1620

gagacccugc uccaccagcu gcgccagcug gagggcauca gcgaggccga cugcgcgcuc     1680

cuggugaaca cccacuacac cggcuggggg cggcuguccc gcaagcuccu gacgaccaag     1740

gccggcgagu gcaagaucag cgacgacuuc gcgccccgga agcacuccau caucgagauc     1800

augcgcgccg aggaccggaa ccugauggag aucaucaccg acaagcagcu cggguucagc     1860

gacuggaucg agcaggagaa ccugggcgcc gagaacggga gcucccugau ggaggugguc     1920

gacgaccucc gcgugagccc caaggugaag cggggcauca uccaguccau ccgccugauc     1980

gacgacauca gcaaggccgu cggcaagcgc cccucccgga ucuuccugga gcucgccgac     2040

gacauccagc cgagcgggcg caccaucucc cgcaagagcc ggcugcagga ccuguaccgg     2100

aacgccaacc ucggcaagga guucaagggg aucgccgacg agcugaacgc gugcuccgac     2160

aaggaccugc aggacgaccg ccucuuccug uacuacacgc agcugggcaa ggacauguac     2220

accggcgagg agcucgaccu ggaccggcug agcuccgccu acgacaucga ccacaucauc     2280

ccccaggccg ugacccagaa cgacagcauc gacaaccgcg ugcucgucgc ccgggccgag     2340

aacgcgcgca agaccgacuc cuucacguac augccccaga ucgccgaccg gaugcgcaac     2400

uucuggcaga uccugcugga caacgggcuc aucagccggg ugaaguucga gcgccugacc     2460

cggcagaacg aguucuccga gcgcgagaag gagcgguucg ugcagcgcag ccuggucgag     2520

acccggcaga ucaugaagaa cguggccacc cucaugcgcc agcgguacgg caacuccgcc     2580

gccgugaucg ggcugaacgc ggagcugacg aaggagaugc accgcuaccu cggcuucagc     2640

cacaagaacc gcgacaucaa cgacuaccac cacgcccagg acgcccugug cgucgggauc     2700

gccggccagu ucgccgcgaa ccggggcuuc uucgccgacg gggaggugag cgacggcgcc     2760

cagaacuccu acaaccagua ccugcgcgac uaccuccggg gguaccgcga gaagcugagc     2820

gccgaggacc ggaagcaggg ccgcgcguuc ggguucaucg ugggcuccau gcggagccag     2880

gacgagcaga agcgcgucaa cccccggacc ggcgaggugg ugugguccga ggaggacaag     2940

gacuaccugc gcaaggucau gaacuaccgg aagaugcucg ugacccagaa ggugggggac     3000

gacuucggcg cccuguacga cgagacccgc uacgccgcca cggacccgaa ggggaucaag     3060

ggcauccccu ucgacggggc caagcaggac accagccugu acggcggcuu cuccagcgcg     3120

aagcccgccu acgccguccu caucgagucc aaggggaaga cccggcuggu gaacgugacc     3180

augcaggagu acagccugcu cggcgaccgc cccuccgacg acgagcugcg gaagguccug     3240

gccaagaaga aguccgagua cgccaaggcg aacauccucc ugcgccacgu gccgaagaug     3300

cagcugaucc gguacggggg cgggcucaug gugaucaaga gcgccggcga gcugaacaac     3360

gcccagcagc uguggcuccc cuacgaggag uacugcuacu ucgacgaccu gagccagggc     3420

aagggguccc uggagaagga cgaccucaag aagcugcugg acagcauccu cggcuccguc     3480

cagugccugu accccuggca ccgcuucacc gaggaggagc uggccgaccu ccacguggcc     3540

uucgacaagc ugcccgagga cgagaagaag aacgugauca cggggaucgu cagcgcgcug     3600

cacgccgacg ccaagaccgc caaccucucc aucgugggca ugaccggcag cuggcggcgc     3660

augaacaaca agagcgggua caccuucucc gacgaggacg aguucaucuu ccagagcccg     3720

uccggccugu ucgagaagcg cgugacgguc ggggagcuga agcggaaggc gaagaaggag     3780

gugaacagca aguaccgcac caacgagaag cggcucccca cccuguccgg cgccagccag     3840

cccuga                                                                3846


<210>  1757
<211>  3861
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1757
augaccaaga acauccuggg ccucgaccug gggaccaaca gcaucggcug ggcccugauc       60

gagguggacg agaacaacac gcccauccgc aucaucgcca uggggucccg gaucaucccg      120

cucagcaccu ccgacaagga ggaguuccag cgcggccuga gcaucaccaa gaaccagucc      180

cggaccguca gccgcacgca gcggaagggg uacgaccgca agcagcugaa gaaguccgac      240

cucaagaaga uccugaacaa guacaacauc uuccccagcg aggagcugcu caagcugccc      300

augcuggacc ucuggaagcu gcgguccgac agcgcgaacc ccaacgagaa caucuccgcc      360

gagcagcugg gccgcauccu cuacaugcug aaccagaagc ggggcuacaa gagcgcccgc      420

uccgaggcca acgccgacaa gaaggacacc gacuacgugc aggcggugaa ggggcgguac      480

gcccagcuga aggagcgcaa ccagaccauc ggccaguacu ucuacgagga gcucuacaac      540

gccaacacca acaacagcua cuuccggauc aaggaggagg ucuacccgcg cgaggccuac      600

acggaggagu ucgacaccau caucaacgcg cagaagaaca agcacgacuu ccugaccgac      660

gagguggugc accagcugcg gaacgagauc aucuacuucc agcgcaagcu caagucccag      720

aaggggcugg ucagcaucug cgaguucgag ggcuucgaga agaccguguu cgacaaggag      780

aagaacaagg acaagguggu cuucgugggg uccaaggugg cccccaagac gagcccccug      840

uuccagcucu gccggaucug ggaggucgug aacaccaucu cccugaagau caagaacccc      900

gagggcagca aguacaagug gggcgagaag accccgaacc ugaaggagaa ggagauccuc      960

gccgacuacc uguucaagaa cgacagccug uccuucacca agcuccugga gauccuggac     1020

cucaacaaga acgacgugua cgucaacaag cagauccuga aggggaucaa gggcaacgag     1080

acguucagcg cccugcacac cgugcucggg gacaacgagc ugcugaacuu cgacgugucc     1140

aucaucccca gcaaccacac cuccaugcuc gucgacaaga agaccggcga gauccuggag     1200

gagcgcgccg gccuggagcu cgacgcgagc cuggagaagg agccccugua ccagcucugg     1260

cacacgaucu acucccugaa ggugcuggac gagugcaaga acgcccucau caagcgguuc     1320

ggguucgacg acgagaccgc cgagaagcug agccacaucg acuucaacaa gcaggccuuc     1380

ggcaacaagu ccaacaagag caugcgcaag auccugcccu uccugaugga gggguacgac     1440

uacucccaga gcugcucccu cgccggcuac aaccacagca acucccugac caaggacgag     1500

cgggagcagc agaucacccu ggaccgccuc gagcugcuga gcaagaacuc ccuccgccag     1560

ccgaucgugg agaagauccu gaaccagaug aucaacgucg ugaacgcgau ccuggagcag     1620

uacgggaagc ucagcgagau ccggguggag cuggcccgcg agcugaagca guccaaggac     1680

gagcggaacg acgccgaccu ccagaacagc aagaacaaga agcugaacga ggagaucggc     1740

aagcgccugg ccgagcucgg ccugcccguc acgaagcggu acauccagaa guacaaguuc     1800

aucuucccca gccuguccaa gaaccucaag gacgcccacg ugaacaacca gugcaucuac     1860

ugcggggaga ccuucaaccu gaccgaggcg cugagcggcg acgccuucga cgucgaccac     1920

aucgugccca aggcccuccu guucgacgac ucccagacca acaaggugcu gguccaccgc     1980

aagugcaaca gcacgaagac caaccagacc gccuacgacu acaucgggac caagggcgag     2040

gagcaggucc ggaucuacuc cgagcgcgug gacgacuggu ucaagcgggg gaucaucagc     2100

uacuccaaga ugcagcgccu caaggugagc cacaaggagu accuggagcg gaagaagcug     2160

ggcaaggaga cggagaccga caagaagcuc ugggagaacu ucaucgaccg ccagcugcgg     2220

gacacccagu acaucucccg caaggcccgg gagauccugc agaaggugug caacaacgug     2280

accacgaccg agggcgggau caccgcgaag cuccgcaacc uguggggcug ggacgacguc     2340

cugaugaacc uccagaugcc gaaguacaag gagcuggggc agaccgugau caaggagugg     2400

accagcgagc acggcaagcg gaagcaccag aaggaggaga ucgagaacug gacgaagcgc     2460

gacgaccacc ggcaccacgc caucgacgcc cugguggucg ccugcaccaa gcagggguuc     2520

auccagcgca ucaacacccu caacuccagc gacaccaagg accugaugca gcgcgagauc     2580

aaggaggcga agaagcugcu cggcgagaac uacaacgaga aggaguucga gaacgacaac     2640

gaguucguga agaaccacaa ggagcggcug ucccagcugg acgacuaccu ccgccugcag     2700

aagcccguga aguucgacac gaaguacguc gagcaggagg ccgacaaggu gcuggugagc     2760

uucaaggccg gcaagaaggu cgccaccauc accaaguuca aggccaccgg gaagaacaag     2820

gacacgggcg ugaucgugcu ccgcggggcg cugcacgagc aguccgucua cggcaagauc     2880

aaggugaucg agaaggacaa gccccugaag uaccucuucg agaacagcaa gaagaucgug     2940

aaccccaaga ucaagguccu gguggaggag cggcuggccg agaacgagaa caacuccaag     3000

cucagccuga gcacccugaa gaagaacccg aucuuccuca acgaggagaa gaccgagauc     3060

cuggagaagg ccuccugcua caacgacgcc accgugcuga aguacaagcu ccagagccug     3120

aaggccuccc aggcggacga caucgucgac gaccggguga aguuccuggu gaaggagcgc     3180

cucgcccagu ucggcaacaa ggagaaggag gccuucaagg acacgcugug gcugaacgag     3240

gagaagcaga uccccaucau caccguccgc cucuucgccc ggcccgacgc caacaaccug     3300

caggcgauca agaaggacaa gaacgggcgg gacaucggcu ucgugcugac cgggaacaac     3360

caccacaucg ccaucuacga ggacaaggac aacaagcucg ugcagcacag cugcaccuuc     3420

uggcacgccg ucgagcgcaa gaagaacaac gugcccgccg ugaucaagaa cuccagcgac     3480

gucuggaacc agcugcugga caaggagcuc ccggaguccu uccugaacaa gcugcccauc     3540

gacaagcuca agcugaaguu cagccugcag cagaacgaga uguucgugcu cggccugcug     3600

caggaggacu ucgaggcggc caucgagaac aacgacaaga cgcuccuguc caagcaccug     3660

uaccucgugu ggagcaucac cgacggggac uacuucuucc ggcaccaccu ggagaccaag     3720

aacaccgagc ugaagaagac ggagggcgcc aaggagucca agcgcuacuu ccggcucagc     3780

accaagggcc ugaucgaccu gaaccccguc aaggugcgcc ucaaccaccu gggggagauc     3840

accaagaucg gcgaguacug a                                               3861


<210>  1758
<211>  3864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1758
augaagaccg ugcugggccu cgaccugggg accaacagca ucggcugggc ccugaucgag       60

cucgacgaga acaacauccc cauccgcauc gucgccaugg ggucccggau caucccgcug      120

agcacguccg acaaggagga guuccagcgc ggccugagca ucaccaagaa ccagucccgg      180

accgugagcc gcacccagcg gaagggguac gaccgcaagc agcucaagaa guccuaccug      240

aagaagaucc ugaacaagua caacaucuuc cccagcgacg agcuccugaa gcugcccaug      300

cucgaccugu ggaagcugcg guccgacagc gcgaacccca acgagaacau cuccgccgag      360

cagcucggcc gcauccugua caugcugaac cagaagcggg gcuacaagag cgcccgcucc      420

gaggccaacg ccgacaagaa ggacacggac uacauccagg cggugaaggg gcgguacgcc      480

cagcucaagg accgcaacca gaccaucggc caguacuucu acggggagcu gaacgacgcc      540

aacgagaaca acagcuacuu ccggaucaag gaggaggucu acccgcgcga ggccuacacc      600

gaggaguucg acaccaucau caacgcgcag aaggacaagc acgacuuccu gacggacgag      660

gugauccacc agcuccggaa cgagaucauc uacuuccagc gcaagcugaa gucccagaag      720

ggccugguga gcaucugcga guucgagggg uucgagaaga ccgucuucga caaggagaag      780

aacaaggaca aggugguguu cgucggcccc aagguggccc ccaagaccag cccccucuuc      840

cagcugugcc ggaucuggga gguggucaac accaucacgc ugaagaucaa gaacccggag      900

ggcagcaagu acaagugggg cgagaagauc cccgacguga aggagaagga gauccucgcc      960

gacuaccugu ucaagaacga gucccugagc uucaccaagc uccuggagau ccugaagcuc     1020

aacaagaaca acguguacuu caacaagcag auccugaagg ggaucaaggg gaacgagacc     1080

uacagcaucc ugcacuccau ccucggcgac agcgagcugc ugaacuucga cgucuccauc     1140

auccccagca accacaccuc caugcucauc gacaagaaga cgggcgagau ccuggaggag     1200

cgcgccgggc uggagcucga caccagccug gagaaggagc cccugaacca gcucuggcac     1260

accaucuacu ccaucaagga ccuggacgag ugcaagagcg cccugaucaa gcgguucggc     1320

uucgacgacg agaccgcgga caagcucucc cgcaucgacu ucaacaagca ggccuucggg     1380

aacaagagca acaaguccau gcggaagauc cugccguucc ugauggaggg cuacgacuac     1440

agccaguccu gcagccuggc cggguacaac cacuccaaca gcuucacgaa ggacgagcgc     1500

gagcagcaga ucacccucga caagcuggag cugcucccca agaacucccu gcgccagccc     1560

aucguggaga agauccugaa ccagaugauc aacgugguca acgccauccu cgagcgguac     1620

ggcaagccca gcgagauccg cguggagcug gcccgggagc ugaagcaguc caaggacgag     1680

cgcaacgaca ccgaccucca gaacagcaag aacaagaagc ugaacgagga gaucggcaag     1740

cggcuggcgg agcucgggcu gccgcugacc aagcgcuaca uccagaagua caaguucauc     1800

uuccccagcc ucuccaagaa ccugaaggac gcccacguga acaaccagug caucuacugc     1860

ggcgagacgu ucaaccugac cgaggcccuc agcggggacg ccuucgacgu cgaccacauc     1920

gugcccaagg cccugcuguu cgacgacucc cagaccaaca aggugcucgu ccaccggaag     1980

ugcaacagca ccaagacgaa ccagaccgcg uacgacuaca ucgccaccaa gggcgacgag     2040

cagguccgcg uguacuccga gcggguggac gacugguuca agcgcgggau caucagcuac     2100

uccaagaugc agcggcugaa ggugagccac aaggaguacc uggagcgcaa gaagcucggc     2160

aaggagaccg agacggacaa gaagcugugg gagaacuuca ucgaccggca gcugcgcgac     2220

acccaguaca ucucccggaa gucccgcgag auccuccaga aggucugcaa caacguggcc     2280

accaccgagg gcggggugac ggccaagcug cggaaccugu ggggcuggga cgacguccuc     2340

augaaccugc agaugcccaa guacaaggag cuggggcaga ccguggugaa ggaguggacc     2400

uccgagcacg gcaagcgcaa gcaccagaag gaggagaucg agaacuggac caagcgggac     2460

gaccaccgcc accacgccau cgacgcgcuc gucguggccu gcaccaagca gggguucauc     2520

cagcgcauca acacgcugaa cagcuccgag accaaggacc ugaugcagca ggagaucaag     2580

gaggccaaga agcuccuggg cgagaacuac aacgagaagg aguucgagaa caacaacgag     2640

agcgugaaga accacaagga gcggcugucc cagcucgacg acuaccugcg ccugcagaag     2700

ccggucaagu ucgacaccaa guacguggag caggaggccg acaaggugcu cgucagcuuc     2760

aaguccggca agaagguggc gaccaucacg aaguucaagg ccaccgggaa gaacaaggac     2820

accggcguga ucgucccccg cggggcccug cacgagcaga acguguacgg caagaucaag     2880

gugaucgaga aggacaagag ccugaaguac cucuucgaga acagcgagaa gaucgucaac     2940

cccaagauca agaacaucgu ggagaucauc cuguccgaga acgagaacaa cagcaagaug     3000

gcccugucca cccucaagaa gaaccccauc uuccugaacg aggagaagac ggaccugcuc     3060

gagaaggcca gcugcuacga guacgugacc guccggaagu acccgcugaa caccaucaag     3120

gcgucccagg ccgacgacau cguggacgag cgggugaagu uccuggucaa ggagcgccuc     3180

acccaguucg gcaacaagga gaaggaggcc uucaaggaca cgcugugguu caacgaggag     3240

aagcagaucc ccaucaucac cgugcggcug uucgcccgcc ccgacgccaa caaccuccag     3300

gcgaucaaga aggacgagag cgggaaggag aucggcuucg uggucauggg gaacaaccac     3360

cacaucgcca ucuacgagga caaggacggc aagcuggugc agcacuccug caccuucugg     3420

cacgccgugg agcggaagaa guacaacauc agcaccguca ucaagaacuc cagcgacgcc     3480

uggaaccagu uccuggacaa cgagcucccc gagccguucc ugaacaagcu gcccgccgac     3540

aaccucaacc ugaaguucuc ccugcagcag aacgagaugu ucauccucgg gcugagccag     3600

gaggaguucg acaaggcgcu gaaguccaac gacaagagcc uccuguccaa gcaccuguac     3660

cucgugugga gcaucuccga gaacaacuac ugguuccgcc accaccugga gacgaagaac     3720

agcgaccuga agaagaccaa cggcgugaag gaguccaagc ggcucuacaa cauccgcagc     3780

cugggcagcc uguucucccu caaccccauc aagauccggc ugaaccaccu gggggagauc     3840

accaagaucg gcgaguacag cuga                                            3864


<210>  1759
<211>  3867
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1759
augcgccugu ucgaggaggg ccacaccgcc gaggaccggc gccucaagcg gaccgcccgc       60

cggcgcauca gccggcgccg gaaccgccug cgguaccugc aggcguucuu cgaggaggcc      120

augacggacc ucgacgagaa cuucuucgcc cgccugcagg aguccuuccu ggugcccgag      180

gacaagaagu ggcaccggca cccgaucuuc gccaagcucg aggacgaggu cgccuaccac      240

gagaccuacc ccaccaucua ccaccuccgc aagaagcugg cggacagcuc cgagcaggcc      300

gaccuccggc ugaucuaccu ggcccucgcc cacaucguga aguaccgcgg gcacuuccug      360

aucgagggca agcugagcac cgagaacacg uccgugaagg accaguucca gcaguucaug      420

gucaucuaca accagaccuu cgugaacggg gagagccggc ucguguccgc gccccugccc      480

gagagcgucc ugaucgagga ggagcucacc gagaaggccu cccgcaccaa gaaguccgag      540

aaggugcugc agcaguuccc gcaggagaag gccaacggcc uguucgggca guuccucaag      600

cugauggugg gcaacaaggc cgacuucaag aaggucuucg gccuggagga ggaggccaag      660

aucacguacg cgagcgagag cuacgaggag gaccucgagg ggauccuggc caaggugggc      720

gacgaguacu ccgacguguu ccuggccgcc aagaacgucu acgacgccgu ggagcucagc      780

accauccugg cggacuccga caagaagucc cacgccaagc ugagcagcuc caugaucgug      840

cgguucaccg agcaccagga ggaccucaag aaguucaagc gcuucauccg ggagaacugc      900

cccgacgagu acgacaaccu guucaagaac gagcagaagg acggguacgc cggcuacauc      960

gcccacgccg ggaaggucag ccagcugaag uucuaccagu acgugaagaa gaucauccag     1020

gacaucgcgg gcgccgagua cuuccucgag aagaucgccc aggagaacuu ccugcgcaag     1080

cagcgcaccu ucgacaacgg cgugaucccc caccagaucc accuggccga gcuccaggcc     1140

aucauccacc ggcaggcggc cuacuacccc uuccugaagg agaaccagga gaagaucgag     1200

cagcugguca cguuccgcau cccguacuac guggggcccc ucagcaaggg cgacgccucc     1260

accuucgccu ggcugaagcg gcagagcgag gagcccaucc gccccuggaa ccugcaggag     1320

accguggacc ucgaccaguc cgcgaccgcc uucaucgagc ggaugacgaa cuucgacacc     1380

uaccugccga gcgagaaggu ccugcccaag cacucccucc uguacgagaa guucauggug     1440

uucaacgagc ugaccaagau cagcuacacc gacgaccgcg ggaucaaggc caacuucucc     1500

ggcaaggaga aggagaagau cuucgacuac cucuucaaga cgcggcgcaa ggugaagaag     1560

aaggacauca uccaguucua ccggaacgag uacaacaccg agaucgucac ccugagcggc     1620

cuggaggagg accaguucaa cgccuccuuc agcaccuacc aggaccugcu caagugcggg     1680

cugacgcgcg ccgagcugga ccaccccgac aacgcggaga agcucgagga caucaucaag     1740

auccugacca ucuucgagga ccggcagcgc auccggaccc agcuguccac cuucaagggc     1800

caguucagcg ccgaggugcu caagaagcug gagcgcaagc acuacacggg guggggccgg     1860

cuguccaaga agcucaucaa cgggaucuac gacaaggaga gcggcaagac cauccuggac     1920

uaccugguca aggacgacgg cguguccaag cacuacaacc gcaacuucau gcagcucauc     1980

aacgacagcc agcuguccuu caagaacgcc auccagaagg cccagagcuc cgagcacgag     2040

gagacccuga gcgagaccgu gaacgagcuc gccgggagcc ccgcgaucaa gaagggcauc     2100

uaccaguccc ugaagaucgu cgacgagcug guggccauca ugggguacgc cccgaagcgg     2160

aucguggucg agauggcccg cgagaaccag acgaccagca ccggcaagcg gcgcuccauc     2220

cagcgccuca agaucgugga gaaggccaug gcggagaucg ggagcaaccu gcugaaggag     2280

cagcccacca cgaacgagca gcuccgggac acccgccugu uccuguacua caugcagaac     2340

ggcaaggaca uguacaccgg cgacgagcuc ucccugcacc ggcugagcca cuacgacauc     2400

gaccacauca ucccccaguc cuucaugaag gacgacagcc ucgacaaccu ggugcugguc     2460

ggguccaccg agaaccgcgg caagagcgac gacgugcccu ccaaggaggu ggucaaggac     2520

augaaggccu acugggagaa gcucuacgcc gccgggcuga ucagccagcg gaaguuccag     2580

cgccugacca agggcgagca gggcgggcuc acgcuggagg acaaggcgca cuucauccag     2640

cggcagcugg uggagacccg ccagaucacc aagaacgugg ccggcauccu cgaccagcgg     2700

uacaacgcca aguccaagga gaagaagguc cagaucauca cccugaaggc cagccugacg     2760

ucccaguucc gcagcaucuu cgggcucuac aaggugcggg aggugaacga cuaccaccac     2820

ggccaggacg ccuaccugaa cugcgucgug gcgaccaccc ugcucaaggu guacccgaac     2880

cuggcccccg aguucgucua cggggaguac cccaaguucc agaccuucaa ggagaacaag     2940

gccacggcca aggccaucau cuacaccaac cugcuccgcu ucuucaccga ggacgagccc     3000

cgguucacca aggacggcga gauccugugg uccaacagcu accugaagac gaucaagaag     3060

gagcucaacu accaccagau gaacaucgug aagaaggugg agguccagaa gggcggguuc     3120

agcaaggagu ccaucaagcc gaagggcccc agcaacaagc ugauccccgu gaagaacggg     3180

cuggaccccc agaaguacgg cggguucgac uccccggugg ucgcguacac cguccucuuc     3240

acccacgaga agggcaagaa gccccugauc aagcaggaga uccugggcau caccaucaug     3300

gagaagacgc gcuucgagca gaaccccauc cucuuccugg aggagaaggg guuccugcgg     3360

ccgcgcgugc ucaugaagcu gcccaaguac acccuguacg aguuccccga gggccggcgc     3420

cggcuccugg ccagcgccaa ggaggcccag aaggggaacc agauggugcu gcccgagcac     3480

cuccugaccc ugcucuacca cgccaagcag ugccugcugc cgaaccaguc cgagagccuc     3540

gcguacgugg agcagcacca gcccgaguuc caggagaucc uggagcgcgu ggucgacuuc     3600

gccgaggugc acacccuggc caaguccaag gugcagcaga ucgucaagcu guucgaggcc     3660

aaccagacgg cggacgugaa ggagaucgcc gccagcuuca uccagcugau gcaguucaac     3720

gccaugggcg cccccuccac cuucaaguuc uuccagaagg acaucgagcg ggcgcgcuac     3780

accagcauca aggagaucuu cgacgccacc aucaucuacc agucccccac ggggcuguac     3840

gagacccgcc ggaagguggu cgacuga                                         3867


<210>  1760
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1760
auguggggcg ugagccuguu cgaggccggg aagaccgccg cggagcgccg gggcuaccgc       60

uccacccggc gccggcucaa ccaccgcaag uuccggcugc gccugcucga ggacauguuc      120

gagaaggaga uccugagcaa ggaccccucc uucuucaucc ggcugaagga ggccuuccuc      180

agcccgaagg acgagcagaa gcaguucaag gggaacaucc uguucaacga caaggacuac      240

acggacgccg acuacuacga gcaguacaag accaucuacc accuccgcua cgaccucauc      300

ucccagcacc ggcaguucga cauccgcgag gucuaccugg ccauccacca ccugaucaag      360

uaccggggcc acuucaucua cgaggaccag accuucacca cggacgggaa ccagcuccag      420

caccacauca aggccaucau caccaugauc aacagcaccc ugaaccgcau caucaucccc      480

gagaccaucg acaucaacgu guucgagaag auccugcucg accggaugau gaaccgcucc      540

agcaagguga aguuccugau cgagcugacg ggcgaggaca agcaggacaa gccccuccug      600

aaggagcugu ucaaccucau cgucggccug aaggcgaagc ccgccuccau cuucgagcag      660

gagaaccugg ccaccaucgu ggagaccaug aacaugagca ccgagcaggu ccagcucgac      720

cugcugacgc ucgccgacgu gcuggcggac gaggaguacg accugcuccu gaccgcccag      780

aagaucuacu ccgccaucau ccuggacgag agcauggacg gguacgagua cuucgccgag      840

gccaagaagg aguccuaccg gaagcaccag gaggagcucg ugcuggugaa gaagaugcuc      900

aagagcaacg cgaucaccaa cgacgagcgc gccaaguucg aguacuucua caccgacuac      960

aucggcgcca aguccagcaa cuacgaggag uccaagaaca ucaagaaggg gcugagcgcc     1020

gccuacggca aguacuccaa ggaggagcgg cuguucaagc acaucgagcu gcuccuggcg     1080

aaggagaacg uccugacgac cguggagcac gcccuccugg agaagaacau caccuucgcc     1140

agccugcucc cgcugcagcg cagcucccgg aacgccguga uccccuacca gguccacgag     1200

aaggagcugg uggccauccu cgagaaccag gcgaccuacu accccuuccu gcuggagcag     1260

aaggacaaca uccacaagcu gcucacguuc cgcauccccu acuacguggg gccgcuggcc     1320

gaccagaagg acagcgaguu cgccuggaug guccgcaagc aggccggcaa gaucaccccc     1380

uucaacuucg aggagauggu ggacaucgac gccuccagcg aggcguucau caagcggaug     1440

accaacaagu gcaccuaccu gauccacgag gacgugaucc ccaagcacuc cuucagcuac     1500

gccaaguucg agguccucaa cgagcugaac aagauccgcc uggacggcaa gcccaucgac     1560

aucccgcuca agaagcggau cuucgagggg cuguuccugg agaagacgaa ggugacccag     1620

accucccuca agaaguggcu ggccgagcac gagcacauga ccgugagcgu cgugcagggc     1680

acgcagaagg agaccgaguu cgccaccucc cugcaggcgu uccaccgcuu cgugaagauc     1740

uucgaccggg agaccgucag caaccccgcc aacgaggaga uguucgagaa gaucaucuac     1800

ugguccacgg uguucgagga caagaagauc augcgccgga agcucagcga guacccccag     1860

cugaccgagc agcagcaggu ccagcuggcc caggugcgcu uccgggggug gggccgccuc     1920

ucccagcggc ugaucaaccg caucaagacc cccgugagcg gcgacgagga ccacaagcug     1980

uccaucaacg agauccucug gcagaccaac gagaacuuca ugcagaucau ccggaacaag     2040

gacuaccugu ucaagaagau caucgaggag caguucgaga acgagacggc ccugcucaac     2100

aagcagcgca ucgacgagcu ggccgcgagc ccggccaaca agaaggggau cuggcaggcc     2160

aucaagaucg ucaaggagcu ggagaaggug cuccagcagc ccgccgagaa caucuucauc     2220

gaguucgccc gguccgacga ggagagcaag cgcuccacgc cccgggacaa guucaucgag     2280

aaggcguacg cccagcugaa gaaggagacc gacaccuuca accuggagca ccucaaggag     2340

cugaagcagc gcagcaagca gcugagcucc cagcggcugu uccuguacuu cauccagaac     2400

ggcaagugca uguacagcgg ggagcaccug gacaucgagc gccucgacuc cuacgaggug     2460

gaccacaucc ugccccagag cuacaucaag gacaacucca ucgagaaccu ggcccucguc     2520

aagaaggugg agaaccagcg caagaaggac agccugcugc ucaacuccag caucaucaac     2580

cagaacuacu cccgguggga gcagcugaag aacgccggcc ugaucgggga gaagaaguuc     2640

cgcaaccuca cccggaccaa gaucaccgac cgcgacaagg agggcuucau cgcccggcag     2700

cugguggaga cccgccagau caccaagcac gucacgcagc ugcuccagca ggaguacaag     2760

gacaccacca agguguucgc gaucaaggcc acccugguga gcggccugcg gcgcaaguuc     2820

gaguucauca agaaccggaa cgucaacgac uaccaccacg cccaggacgc cuuccucgug     2880

gcguuccugg ggacgaacau caccuccaac uacccgaaga ucgagaugga guaccuguuc     2940

aagggcuacc agcacuaccu caacgaggug ggcaagagcg ccgccaagcc caaguucacc     3000

uucaucgucg agaaccuguc caagcagcag uacaacagca ccacggggga ggugaagugg     3060

aaccccgagg uggacaucgc caagcugaag cgcauccuca acuucaagca gugcaacauc     3120

guccggaagg uggaggagca guccggggcc cuguucaagg agaccaucua ccccguggag     3180

gagagcucca gcaagaccau cccgcugaag aagcaccucg acaccgcgau cuacggcggc     3240

uacacggccg ucaacuacgc cagcuacgcc cugauccagu ucaagaaggg gcgcaagcug     3300

aagcgggaga ucaucggcau cccccucgcc gugcagaccc gcaucgacaa cuccgagacc     3360

agccugcagg cguacaucgc cgagcagauc aaguccgagg uggagauccu gaacgggcgg     3420

auccucaagu accagcugau cagcaacaac ggcaaccgcc uguacaucgc cgggcccucc     3480

gagcggcaca acgcccgcca gcucaucguc agcgacgagg ccgcgaaggu gaucuggcug     3540

aucuccacca agcaggccga cgaggccaug uuccugaagu acuaccggcu cgagcaccug     3600

gaggccgugu ucgaggagcu gauccgcaag caggcggccg acuaccagau cuucgagaag     3660

cucaucaaga agaucgaggu caacaaggug uacuucuaca gcugcacgua caacgagaag     3720

gugaagguca ucgaggagcu gcugaagauc acccaggcca acgccaccaa cggcgaccuc     3780

aagcugcuga agauguccaa ccgggagggc cgccucggga gcguguccgu ggcccuggac     3840

uucaagauca ucaaccagag cgucaccggc cuguaccagu ccaucgagga cuacaacaac     3900

uga                                                                   3903


<210>  1761
<211>  3930
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1761
augggcagcg ugggguucgc cggcaucgac aaccaguaca accugguccg caccaagggg       60

aagaacguga ucggcgugcg gcuguucgac gaggccgacu ccgcggccga gcgccggggg      120

caccgcacca accggcgccg gcugcagcgc cggcgcuggc ggcugcgccu ccuggacgac      180

aucuucgcca agccccugca ggccgucgac ccgaacuucc ucgcccggcu gaaguacagc      240

uacgugaaca agaaggacca gggccagcag gaccacuacu acggcgggua cguguucggc      300

uccacggcgg ccgaccaggc cuaccaccag gccuacccca ccaucuacca ccuccgcaag      360

cggcucaugg aggacgacca gaagcacgac cugcgcgagg ucuaccuggc gauccaccac      420

aucgugaagu accgggggaa cuuccucaac ccccagagcu cccuggacau cgaccagcag      480

uucgacguga ccgacuucgc ccaggcccug gcccgcuucg ccgaccacca ggcgcucagc      540

ugggcccugg aggcccccau ccgguuccug gaggccgagc ucgccaccgg ccuguccaac      600

agcgcgcgcg ucgacgccgc caucgaggcc uucuccuucg acacgaaggu ggaccgggcc      660

gcgaucaagg agaugcugaa ggggcucagc ggcaaccaga ucgacuucac caagcuguuc      720

gugaacgucg acuccgccga cugggaccag gaggagcgca agcaguggaa gaugaagcug      780

agcgaggagg acuucgacga gcaggcccuc ccgauccugg agcgccuguc ccaggacgag      840

accgaguucu uccucgccau caagcgggcc uacgacggca ucgcgcugau gcgcuuccug      900

ggggacgagc agagccucuc cagcgccaug aucaaggccu acgaggacca ccggcgcgac      960

cugaccuucc ugaagaccca ggugcggacg ccccagaacc gccaggcccu cuccgagggc     1020

uacaccaacu accugagcgu ggacgacaag aagcacaagc gcggggcgaa ggagcuggcc     1080

cagcucaucg aggccuccga cgccagcgag caggacaagg ccaccaugcu ggaccggauc     1140

gcgaacgacc aguucgcccc caagcagcgg acgaaggcca acggccugau ccccuaccag     1200

cuccaccugg ccgagcugaa gaagauccuc gccaagcagg gccaguacua cccguuccug     1260

cuggacaccu ucgcgaagca ggggcagagc gucaacaaga ucgaggagcu cgugcaguuc     1320

cgcgugcccu acuacgucgg ccccauggug cccaaguccg agaccgccgg gaacgccgag     1380

aaccacuggg uggagaagaa cgacggccag accaagguca gcgugacgcc guggaacuuc     1440

gaccaggugu ucaaccggga ccgcgccgcc aaguccuuca ucgaccggcu gaccgggacc     1500

gacaccuacc ugaucggcga gcccacgcuc ccccgccaca gccugaccua cgagaccuuc     1560

accguccuga acgagcugaa caacauccgg aucgacggca agcgccuccc cguggagacg     1620

aagcaggcga ucgucgagga ccuguucaag aaguaccggc uggugaccaa gaagcgccuc     1680

caggacuacu ucgccuccuu cgggaagcgg gagguggagc ugaccggccu ggccgacgag     1740

agccgcuuca ccuccagccu cacguccuac cacgaccugc aggggcugcu cggcaccgac     1800

uucaucacca acccgcagaa ccacagccug cuggagaaga ucgucgagau ccagaccgug     1860

uucgaggacu ccgacaucgc cgagcgcgag cucgggaagc ugggccugga gcagaagcuc     1920

aucccccggc uggcgaagaa gcacuacacg ggcuggggga accugagccg caagcuccug     1980

gacaccuccu ucauccacga ccccgagcgg cccgaggagc cggugagcau cauggaccug     2040

cucuacacca ccaacaagaa cuucauggag auccugcacg acuccgagua cggcgucgag     2100

gaguggcuca agagccagaa caugaucgac gaccagaagg acauccagau gcgcaucgac     2160

gagcugacga ccucccccgc caacaagcgg gggaucaagc aggccuucaa cgugcuggac     2220

gacaucaccc aggccauggg cgaggagccc gccuacgugu accuggaguu cgcgcgcgag     2280

aagcaggcca gccggcgcac cgucucccgg aagaagcgcc ucgagacccu guacaagaac     2340

gccgcccuga agacggaguu caaggccauc aaggaggcgc ucgccgagga gagcgacgac     2400

cggaugcagg acgaccgccu guaccuguac uacgcccagc ucgggcggga cauguacacc     2460

ggccagagca ucuccaucga ccagcugagc ucccacuacg acaucgacca caucgugccc     2520

cgcgccuuca ucaaggacga cagccuggag aacaaggugc ucgucaaccg gaccgacaac     2580

gcccgcaaga ccgacuccgc gacguucacc gccgacguga aggccaaggc cuucccgcug     2640

uggcagcagc ugaagaagcu cggccugauc agcgcgaaga aguuccggcu gcucacccgc     2700

accggggacu ucacggagau ggagcgggag cgcuucaucg cccggcagcu gguggagacc     2760

cgccagauca ucaagaacgu cgccgcccug aucgagggcc acuucuccca gacccaggcc     2820

guggcgaucc gcgccgaggu gaccggggag cuccggcagc ugacgcagau caagaaggac     2880

cgcgacauca acgacuacca ccacgcccag gacgcccugc ucgucgccac cgcgggcacc     2940

uaccugcacc ggcacuuccc caagcgcgac gcccgguuca ucuacaacga guucgacuac     3000

uacacccagc acuggcugaa gaaccagggc gagaaccgcc ggcgccaccc cuacagcuuc     3060

guggugggga cgauguccaa gggcaacgag gacuggaccc ccgacaaccu caacuaccug     3120

cggaagguca ugcaguacaa gaccaugcug augacccgca agccgguggg gcccgagggc     3180

gcccucuaca aggagacgcu gaucgccgcc gaccccaaga agcggcuggu gggggcgagc     3240

aaggagcgcc aggacccgac caucuacggc ggcuacacca aggaguccag cgccuacaug     3300

ucccucgucc gggccggggg caagaaccag cuggugaaga uccccgugcg caucgccaac     3360

gagauccaca gcgggcagcg gaagcuggac gacuacgucc aggccaaggu gaagaaguuc     3420

gagcgcaucc uccugcccaa gaucucccug ggccagcucg uggaggacga ggggcagcgg     3480

uucuaccugg cgaccaacga gaugaagcac aacgccaagc agcuguggcu cgaccagaag     3540

gucgugacga ccuacaagcg ccugaccgcc gagagccccg uggaggacuu ccugaccguc     3600

uucgacgccc ucacguccag cgcgaccauc caccacuuca aguucuacca gcgggaccug     3660

gagcugcucc gcgacaaccg cgccggcuuc caggaccugg ccaaggccac ccagcugaag     3720

gugcucaagg acgugcugua cgagcugcac gacaacgccg gcuggcggga cccgaucaag     3780

caguacuuca aggagaucgg gcucaagguc cgcaugugga ccaagcugca gaaggagggc     3840

gggaucaagc ugacggacca ggcggagcuc aucuaccaga gccccuccgg ccuguucgag     3900

aagcggcgcc gggugcagga ccugcucuga                                      3930


<210>  1762
<211>  3939
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1762
augcagaacg gcuuccuggg gaucaccgug agcuccgagc aggucggcug ggccgugacc       60

aaccccaagu acgagcucga gcgcgccagc cggaaggacc uguggggcgu gcgccuguuc      120

gacaaggcgg agacggccga ggaccggcgc auguuccgga ccaaccgccg gcucaaccag      180

cgcaagaaga accggaucca cuaccugcgc gacaucuucc acgaggaggu caaccagaag      240

gacccgaacu ucuuccagca gcuggacgag uccaacuucu gcgaggacga ccggaccgug      300

gaguucaacu ucgacaccaa ccucuacaag aaccaguucc ccacggugua ccaccuccgc      360

aaguaccuga uggagaccaa ggacaagccc gacauccggc ucgucuaccu ggccuucagc      420

aaguucauga agaaccgcgg gcacuuccug uacaagggga accucggcga ggugauggac      480

uucgagaacu ccaugaaggg cuucugcgag agccuggaga aguucaacau cgacuucccc      540

acccuguccg acgagcaggu caaggaggug cgggacaucc ucugcgacca caagaucgcc      600

aagaccguga agaagaagaa caucaucacg aucaccaagg ucaagagcaa gaccgccaag      660

gcguggaucg ggcuguucug cggcugcucc gugccgguga aggugcuguu ccaggacauc      720

gacgaggaga ucgucaccga ccccgagaag aucagcuucg aggacgccuc cuacgacgac      780

uacaucgcca acaucgagaa gggggugggc aucuacuacg aggccaucgu gagcgcgaag      840

augcuguucg acugguccau caugaacgag auccuggggg accaccagcu gcucagcgac      900

gccaugaucg ccgaguacaa caagcaccac gacgaccuga agcgccugca gaagaucauc      960

aagggcacgg gcucccggga gcucuaccag gacaucuuca ucaacgacgu cagcgggaac     1020

uacgugugcu acgugggcca cgccaagacc auguccagcg ccgaccagaa gcaguucuac     1080

accuuccuga agaaccgccu gaagaacguc aacgggaucu ccagcgagga cgcggagugg     1140

aucgacaccg agaucaagaa cggcacgcuc cugcccaagc agaccaagcg ggacaacagc     1200

gugauccccc accagcugca gcuccgcgag uucgagcuga uccuggacaa caugcaggag     1260

auguacccgu uccucaagga gaaccgggag aagcugcuga agaucuucaa cuucgugauc     1320

cccuacuacg ucggcccccu caagggggug guccgcaagg gcgaguccac caacuggaug     1380

gugcccaaga aggacggggu gauccacccg uggaacuucg acgagauggu cgacaaggag     1440

gccagcgccg agugcuucau cucccgcaug accggcaacu gcagcuaccu guucaacgag     1500

aaggugcugc ccaagaacuc ccuccuguac gagacguucg aggugcugaa cgagcucaac     1560

ccccugaaga ucaacgggga gcccaucagc gucgagcuga agcagcggau cuacgagcag     1620

cuguuccuga ccggcaagaa ggugaccaag aagucccuga ccaaguaccu caucaagaac     1680

ggcuacgacg aggacaucga gcugagcggg aucgacaacg aguuccacuc caaccucaag     1740

agccacaucg acuucgagga cuacgacaac cuguccgacg aggaggugga gcgcaucauc     1800

cuccggauca cggucuucga ggacaagcag cugcugaagg acuaccucaa ccgcgaguuc     1860

gugaagcuga gcgaggacga gcggaagcag aucugcuccc ugagcuacaa gggcuggggg     1920

aaccucuccg agaugcugcu gaacggcauc accgugaccg acagcaacgg ggucgaggug     1980

uccgugaugg acaugcucug gaacaccaac cugaaccuga ugcagauccu cagcaagaag     2040

uacggcuaca aggccgagau cgagcgcuac aacaaggagc acgagaagaa caucuacaac     2100

cgggaggacc ugauggacua ccugaacauc ccgcccgccc agcgccggaa ggucaaccag     2160

cucaucacga ucgugaaguc ccugaagaag cgcugcggcg ugcccaacaa gaucuucuuc     2220

aaggucagcc gggagcacca ggacgacccc aagcgcacca gcucccggaa ggagcagcug     2280

aaguaccucu acaagagccu gaaguccgag gacgagaagc accugaugaa ggagcucgac     2340

gagcugaacg ggcacgagcu gagcaacgac aagguguacc ucuacuuccu gcagaagggc     2400

cgcugcaucu acuccgggaa gaagcugaac cucagccggc ugcgcaaguc caacuaccag     2460

aacgacaucg acuacaucua cccgcugagc gcggugaacg accgguccau gaacaacaag     2520

guccucaccg gcauccagga gaaccgcgcc gacaaguaca ccuacuuccc cguggacccc     2580

gagauccaga agaagaugaa gggguucugg auggagcugg ugcugcaggg cuucaugagc     2640

aaggagaagu acuuccggcu cucccgcgag aacgacuuca gcgagaacga gcuggucucc     2700

uucaucgagc gcgagaucag cgacaaccag caguccggcc ggaugaucgc cagcgugcug     2760

caguacuacu uccccgaguc caagaucgug uucgucaagg agaagcucau cagcagcuuc     2820

aagcgcgacu uccaccugau cuccagcuac gggcacaacc accuccaggc cgccaaggac     2880

gcguacauca cgaucguggu gggcaacguc uaccacacca aguucaccau ggacccggcc     2940

aucuacuuca agaaccacaa gcggaaggac uacgaccuca accgccuguu ccuggagaac     3000

aucucccggg acgggcagau cgccugggag agcggccccu acggcuccau ccagaccgug     3060

cgcaaggagu acgcccagaa ccacaucgcc gugacgaagc gggucgugga ggugaagggc     3120

ggccucuuca agcagaugcc ccugaagaag gggcacgggg aguacccccu gaagaccaac     3180

gacccgcgcu ucgggaacau cgcgcaguac ggcggcuaca ccaacgucac cgggagcuac     3240

uucgugcucg uggaguccau ggagaagggc aagaagcgga ucagccugga guacgucccc     3300

guguaccugc acgagcgccu cgaggacgac cccgggcaca agcugcugaa ggaguaccuc     3360

guggaccacc ggaagcugaa ccacccgaag auccugcucg ccaagguccg caagaacucc     3420

cugcugaaga ucgacggcuu cuacuaccgg cucaacgggc gcagcggcaa cgcccugauc     3480

cugaccaacg ccguggagcu caucauggac gacuggcaga cgaagaccgc gaacaagauc     3540

uccggcuaca ugaagcggcg cgccaucgac aagaaggccc ggguguacca gaacgaguuc     3600

cacauccagg agcuggagca gcuguacgac uucuaccucg acaagcugga gaacgggguc     3660

uacaagaacc gcaagaacaa ccaggccgag cugauccaca acgagaagga gcaguucaug     3720

gagcucaaga ccgaggacca gugcgugcug cugaccgaga ucaagaagcu guucgugugc     3780

agccccaugc aggccgaccu gacgcugauc ggcgggucca agcacaccgg caugaucgcg     3840

augagcucca acgucaccaa ggccgacuuc agcaucaucg ccgaggaccc ccucgggcug     3900

cggaacaagg ugaucuacuc ccacaugggc gagaaguga                            3939


<210>  1763
<211>  3939
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1763
augcagaacg gcuuccuggg gaucaccgug agcuccgagc aggucggcug ggccgugacc       60

aaccccaagu acgagcucga gcgcgccagc cggaaggacc uguggggcgu gcgccuguuc      120

gacaaggcgg agacggccga ggaccggcgc auguuccgga ccaaccgccg gcucaaccag      180

cgcaagaaga accggaucca cuaccugcgc gacaucuucc acgaggaggu caaccagaag      240

gacccgaacu ucuuccagca gcuggacgag uccaacuucu gcgaggacga ccggaccgug      300

gaguucaacu ucgacaccaa ccucuacaag aaccaguucc ccacggugua ccaccuccgc      360

aaguaccuga uggagaccaa ggacaagccc gacauccggc ucgucuaccu ggccuucagc      420

aaguucauga agaaccgcgg gcacuuccug uacaagggga accucggcga ggugauggac      480

uucgagaacu ccaugaaggg cuucugcgag agccuggaga aguucaacau cgacuucccc      540

acccuguccg acgagcaggu caaggaggug cgggacaucc ucugcgacca caagaucgcc      600

aagaccguga agaagaagaa caucaucacg aucaccaagg ucaagagcaa gaccgccaag      660

gcguggaucg ggcuguucug cggcugcucc gugccgguga aggugcuguu ccaggacauc      720

gacgaggaga ucgucaccga ccccgagaag aucagcuucg aggacgccuc cuacgacgac      780

uacaucgcca acaucgagaa gggggugggc aucuacuacg aggccaucgu gagcgcgaag      840

augcuguucg acugguccau ccugaacgag auccuggggg accaccagcu ccugagcgac      900

gccaugaucg ccgaguacaa caagcaccac gacgaccuga agcgccucca gaagaucauc      960

aagggcacgg gcucccggga gcuguaccag gacaucuuca ucaacgacgu cagcgggaac     1020

uacgugugcu acgugggcca cgccaagacc auguccagcg ccgaccagaa gcaguucuac     1080

accuuccuga agaaccgccu caagaacguc aacgggaucu ccagcgagga cgcggagugg     1140

aucgacaccg agaucaagaa cggcacgcug cugcccaagc agaccaagcg ggacaacagc     1200

gugauccccc accagcucca gcugcgcgag uucgagcuga uccucgacaa caugcaggag     1260

auguacccgu uccugaagga gaaccgggag aagcugcuca agaucuucaa cuucgugauc     1320

cccuacuacg ucggcccccu gaagggggug guccgcaagg gcgaguccac caacuggaug     1380

gugcccaaga aggacggggu gauccacccg uggaacuucg acgagauggu cgacaaggag     1440

gccagcgccg agugcuucau cucccgcaug accggcaacu gcagcuaccu guucaacgag     1500

aaggugcucc ccaagaacuc ccugcuguac gagacguucg aggugcucaa cgagcugaac     1560

ccccugaaga ucaacgggga gagcaucucc gucgagcuca agcagcggau cuacgagcag     1620

cuguuccuga ccggcaagaa ggugaccaag aagucccuca ccaaguaccu gaucaagaac     1680

ggcuacgaca aggacaucga gcugagcggg aucgacaacg aguuccacag caaccugaag     1740

ucccacaucg acuucgagga cuacgacaac cucagcgacg aggaggugga gcagaucauc     1800

cugcgcauca cggucuucga ggacaagcag cugcucaagg acuaccugaa ccgggaguuc     1860

gugaagcugu ccgaggacga gcgcaagcag aucugcagcc ucuccuacaa gggcuggggg     1920

aaccugagcg agaugcugcu caacggcauc accgugaccg acuccaacgg ggucgaggug     1980

agcgugaugg acaugcugug gaacaccaac cugaaccuca ugcagauccu guccaagaag     2040

uacggcuaca aggccgagau cgagcacuac aacaaggagc acgagaagac gaucuacaac     2100

cgggaggacc ugauggacua ccucaacauc ccgcccgccc agcgccggaa ggucaaccag     2160

cugaucacca ucgucaagag ccugaagaag accuacggcg ugcccaacaa gaucuucuuc     2220

aaggugagcc gcgagcacca ggacgacccc aagcggaccu ccagccgcaa ggagcagcuc     2280

aaguaccugu acaagucccu caagagcgag gacgagaagc accugaugaa ggagcuggac     2340

gagcugaacg ggcacgagcu cuccaacgac aagguguacc uguacuuccu gcagaagggc     2400

cggugcaucu acagcgggaa gaagcucaac cugucccgcc ugcgcaagag caacuaccag     2460

aacgacaucg acuacaucua cccccucucc gcggugaacg accggagcau gaacaacaag     2520

guccugacgg gcauccagga gaaccgggcc gacaaguaca ccuacuuccc gguggacccc     2580

gagauccaga agaagaugaa gggguucugg auggagcugg ugcuccaggg cuucaugucc     2640

aaggagaagu acuuccgccu gagccgggag aacgacuucu ccgagaacga gcuggucagc     2700

uucaucgagc gcgagaucuc cgacaaccag cagagcggcc ggaugaucgc cuccgugcuc     2760

caguacuacu uccccgagag caagaucgug uucgucaagg agaagcugau cagcuccuuc     2820

aagcgcgacu uccaccugau cagcuccuac gggcacaacc accuccaggc cgccaaggac     2880

gcguacauca ccaucguggu gggcaacguc uaccacgcca aguucaccau ggaccccgcc     2940

aucuacuuca agaaccacaa gcgcaaggac uacgaccuga accggcuguu ccucgagaac     3000

aucagccgcg acgggcagau cgccugggag uccggcccgu acggcagcau ccagacggug     3060

cggaaggagu acgcccagaa ccacaucgcg gugaccaagc gcgucgugga ggugaagggc     3120

gggcuguuca agcagaugcc ccugaagaag gggcacggcg aguacccccu caagaccaac     3180

gacccccggu ucgggaacau cgcccaguac ggcggcuaca ccaacgucac cggguccuac     3240

uucgugcugg uggagagcau ggagaagggc aagaagcgca ucucccugga guacgucccg     3300

guguaccucc acgagcggcu ggaggacgac cccgggcaca agcugcucaa ggaguaccug     3360

guggaccacc gcaagcugaa ccaccccaag auccuccugg ccaagguccg gaagaacagc     3420

cugcucaaga ucgacggcuu cuacuaccgc cugaacgggc gguccggcaa cgcccugauc     3480

cucacgaacg cgguggagcu gaucauggac gacuggcaga ccaagaccgc caacaagauc     3540

agcggcuaca ugaagcgccg ggccaucgac aagaaggccc gcguguacca gaacgaguuc     3600

cacauccagg agcuggagca gcucuacgac uucuaccugg acaagcugga gaacgggguc     3660

uacaagaacc ggaagaacaa ccaggccgag cucauccaca acgagaagga gcaguucaug     3720

gagcugaaga ccgaggacca gugcgugcug cucacggaga ucaagaagcu guucgugugc     3780

uccccgaugc aggcggaccu gacccucauc ggcgggagca agcacaccgg caugaucgcc     3840

auguccagca acgucaccaa ggccgacuuc gccgugaucg ccgaggaccc ccuggggcug     3900

cgcaacaagg ugaucuacuc ccacaugggc gagaaguga                            3939


<210>  1764
<211>  3942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1764
augcacuacc uggacgagau cuucgcgccc caccuccagg agguggacga gaacuuccug       60

cgccggcuga agcagagcaa cauccacccg gaggaccccg ccaagaacca ggccuucauc      120

ggcaagcucc uguuccccga ccugcucaag aagaacgagc gcggguaccc cacccugauc      180

aagaugcggg acgagcugcc ggucgagcag cgcgcccacu accccgugac caacaucuac      240

aagcuccggg aggccaugau caacgaggac cgccaguucg accugcggga gguguaccug      300

gccguccacc acaucaugaa guaccgcggc cacuuccuca acaacgccuc cguggacaag      360

uucaaggugg ggcggaucga cuucuacaag agcuucaacg uccugaacga ggcguacgag      420

gagcugcaga acggcgaggg guccuucacg aucgagccca gcaaggugga gaagaucggc      480

cagcuccugc uggacaccaa gaugcgcaag cucaaccggc agaaggccgu ggccaagcug      540

cuggagguca agguggccga caaggaggag accaagcgca acaagcagau cgcgaccgcc      600

auguccaagc ucgugcuggg cuacaaggcc gacuucgcca cggucgccau ggcgaacggg      660

aacgagugga agaucgaccu gagcuccgag accagcgagg acgagaucga gaaguuccgg      720

gaggagcucu ccgacgccca gaacgacauc cugaccgaga ucaccagccu guucucccag      780

aucaugcuca acgagaucgu gcccaacggc augagcaucu ccgagagcau gauggaccgc      840

uacuggacgc acgagcggca gcuggccgag gugaaggagu accuggccac ccagccggcc      900

uccgcgcgca aggaguucga ccaggucuac aacaaguaca ucgggcaggc ccccaaggag      960

cggggcuucg accucgagaa ggggcugaag aagauccuga gcaagaagga gaacuggaag     1020

gagaucgacg agcuccugaa ggccggcgac uuccugccca agcagcgcac cuccgccaac     1080

ggcgugaucc cccaccagau gcaccagcag gagcucgacc ggaucaucga gaagcaggcc     1140

aaguacuacc cguggcuggc gaccgagaac cccgccacgg gggagcgcga ccggcaccag     1200

gccaaguacg agcuggacca gcucgugagc uuccgcaucc ccuacuacgu cggcccccug     1260

gugaccccgg aggugcagaa ggccaccucc ggggccaagu ucgcgugggc caagcgcaag     1320

gaggacggcg agaucacccc cuggaaccug ugggacaaga ucgaccgggc cgagagcgcc     1380

gaggcguuca ucaagcgcau gacggucaag gacaccuacc uccugaacga ggacgugcug     1440

cccgccaaca gccuccugua ccagaaguac aacgugcuga acgagcucaa caacguccgg     1500

gugaacggcc gccggcuguc cgucgggauc aagcaggaca ucuacaccga gcuguucaag     1560

aagaagaaga ccgugaaggu gagcgacguc gccucccugg ugauggccaa gacgcgcggc     1620

gugaacaagc ccagcgucga ggggcucucc gacccgaaga aguucaacag caaccuggcc     1680

accuaccugg accucaaguc caucgugggc gacaaggugg acgacaaccg guaccagacc     1740

gaccuggaga acaucaucga guggcgcagc gucuucgagg acggggagau cuucgcggac     1800

aagcugaccg agguggagug gcucacggac cagcagcggu ccgcccuggu gaagaagcgc     1860

uacaagggcu ggggccggcu gagcaagaag cuccugaccg ggaucgucga cgagaacggc     1920

cagcgcauca ucgaccugau guggaacacc gaccagaacu ucaugcagau cgugaaccag     1980

cccguguuca aggagcagau cgaccagcuc aaccagaagg ccaucaccaa cgacgggaug     2040

acgcugcggg agugggucga guccgugcug gacggcgccu acaccagccc ccagaacaag     2100

aaggccaucu ggcaggucgu gcgcguggug gaggacaucg ucaaggcggu ggggaacgcc     2160

cccaagucca ucagcaucga guucgcccgg aacgagggca acaagggcga gaucacccgc     2220

ucccggcgca cccagcucca gaagcuguuc gaggaccagg cccacgagcu ggugaaggac     2280

acgagccuca ccgaggagcu ggagaaggcc ccggaccugu ccgaccggua cuaccucuac     2340

uucacccagg gcgggaagga cauguacacc ggggacccca ucaacuucga cgagaucagc     2400

acgaaguacg acaucgacca cauccugccc caguccuucg ucaaggacga cagccuggac     2460

aaccgcgugc ucgugagccg cgcggagaac aacaagaagu ccgaccgggu ccccgccaag     2520

cuguacgccg ccaagaugaa gccguacugg aaccagcugc ucaagcaggg ccugaucacc     2580

cagcgcaagu ucgagaaccu gaccauggac guggaccaga acaucaagua ccggagccuc     2640

ggguucguga agcgccagcu ggucgagacc cgccagguga ucaagcugac cgcgaacauc     2700

cucggcucca uguaccagga ggccggcacg gacaucaucg agacccgggc cgggcugacc     2760

aagcagcugc gggaggaguu cgaccucccc aaggugcgcg aggucaacga cuaccaccac     2820

gccguggacg ccuaccugac cacguucgcg ggccaguacc ugaaccggcg cuaccccaag     2880

cuccggagcu ucuucgugua cggggaguac augaaguuca agcacggcuc cgaccugaag     2940

cugcgcaacu ucaacuucuu ccacgagcuc auggagggcg acaagagcca ggggaagguc     3000

guggaccagc agaccggcga gcugaucacc acccgggacg agguggccaa guccuucgac     3060

cgccugcuca acaugaagua caugcugguc agcaaggagg ugcacgaccg guccgaccag     3120

cuguacgggg ccacgaucgu gaccgccaag gagagcggca agcucaccuc cccgaucgag     3180

aucaagaaga agcgccccgu cgaccuguac ggggccuaca ccaacggcac gagcgcguuc     3240

augaccauca ucaaguucac cggcaacaag cccaaguaca aggugaucgg ggugcccacc     3300

acguccgccg ucggccugaa gcgggugggg aagccgggca gcgaguccua caaccaggag     3360

cuccaccgca ucaucaagag caaccccaag gugaagaagg acuucgagau cgucgugccc     3420

cacguguccu acagccagcu gaucgucgac gacgacugca aguucacccu ggccagcgac     3480

accuaccagc accccgccac ccagcucgug cuguccaagg agagcaugga gaucaucgcc     3540

gacgacuuca aguucgucaa ggagaacacg gcgaccgccg acgagcagcu ggugcgggug     3600

uucgacgaga ucgucaacca gaugaaccac uacuucacca ucuucgacca gcgcuccaac     3660

cggcagaagg ugaccaaggc ccgcgacaag uucgugagcc ucccgacgga guccgaguac     3720

gagggggcca agaagaccca ggucggcaag accgagguga ucaccaaccu gcugaugggc     3780

cuccacgcga acagcgccca gggggaccug aaggugcugg gccucuccac guucggguuc     3840

uuccagagcu ccggcgggcu gaaccugagc gaggacgcca ugaucgucua ccaguccccc     3900

accggccucu ucgagcgccg gaucugccug aaggacaucu ga                        3942


<210>  1765
<211>  3942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1765
augagcuuca aggagaacuc caagaucuac uacggccugg acaucgggac cgacagcgug       60

ggcuggucca ccacgaacga gaaguacgag cuccugcgcu acaagaacaa ccugaugugg      120

ggcgucagcc ucuucgaggc cgccucccug gcggaggagc ggcgcgggca ccggacccag      180

cgccggcgcu acgaccggcg ccagcagcgg guggcccugc ucggggagcu guucgccaag      240

gagauccuga agaccgaccc cgacuucuuc cuccgccuga aggagagcuc ccuguacccg      300

gaggaccgga ccagcaagaa cgugaacacg uacuucgacg acgccgacuu caaggacucc      360

gacuacuuca agauguaccc caccguccac caccucauca aggagcugag cgaguccaac      420

aagccccacg acgugcgccu gguguaccuc gccugcgcgu ucaucgucgc ccaccggggc      480

cacuuccuga acggcgccga cgagaacaac gugcaggccg ugcuggacuu cgacagcucc      540

uacugcgagu ucaccgacug guucaagagc aacgacaucg aggacaaccc cuucuccgag      600

agcgucaaga acgaguucuc cgugauccuc cgcaagaaga ucgggaucac cgcgaaggag      660

cgggagauca agaaccugcu guucggcacg accaagaccc cggacugcua caaggacgag      720

gaguacccca ucgacaucga cgugcucauc aaguucauca gcgggggcaa gaccaaccug      780

gccaagcugu uccgcaaccc cuccuacgac gagcucgaca uccagacggu cgaggugggg      840

aaggccgacu ucgccgacac caucgaccug cuggccagca gcauggagga caccgacgug      900

ccccuccugu ccgcggucaa ggccauguac gacuggagcc ugcucaucga cgugcugaag      960

ggccagaaga ccaucuccga cgccaaggug ugcgaguacg agcagcacaa gagcgaccug     1020

aaggcccuca aguacaucgu ccggaaguac cuggaccgcg cccaguacga cgagaucuuc     1080

cggacggcgg gcgagaagcc gaacuacgug uccuacagcu acaacgugac cgacgucaag     1140

cugaagcagc uccccuccaa cuucaagaag aagaacagcg aggaguucug caaguacauc     1200

aaguccaagc uggagaagau caagcccgag agcgacgacg aggccgugua caacgagcug     1260

aucgagaagu gcaacuccaa gacccucugc cccaagcagg ucaccgacga gaaccgcgug     1320

aucccguacc agcuguacua ccacgagcug agcaugaucc ucgacaaggc cuccgccuac     1380

cuggacuucc ugaacaagac ggaggacggg aucagcguga agcagaagau ccucacccug     1440

augaaguucc ggauccccua cuucgucggc ccccugguga agcgcaacga gaccgacaac     1500

guguggaucg uccgcaaggc cgaggggcgg aucuaccccu ggaacuucga gaacauggug     1560

gacuucgaca aguccgagga cggcuucauc cgccggauga ccugcaagug cacguaccuc     1620

gcgggcgagg acgugcugcc gaaguacagc cugcuguacu ccaaguacac cguccucaac     1680

gagaucaaca acaucaaggu gaacggggag aagaucagcc ccgagcugaa gcaggacauc     1740

uucaacgagc uguucaugaa gaccucccgc gugaccguca agaagaucac ggagcuccug     1800

aagcggaagg gcgccuucag cgaggagaag ggggacagcc uguccggcgu ggacaucaac     1860

aucaagagcu cccucaagag cuaccuggac uuccgccggc ugcucgagaa cgggucccug     1920

agcgagaacg acguggagcg caucaucgag cggaucaccg ucaccaccga caagccccgc     1980

cugguguccu ggcucaaggc cgaguacagc gaccugcccg ccgaggacau ccgguacauc     2040

ucccgccuga gcuacaagga cuacggccgg cucuccgcca agcugcugac gggcugcuac     2100

gagcucgaca ccaacaccgg ggagaucggc gggcgcagca ucaucgacuu caugugggcg     2160

gagaacauca accugaugca gauccugucc gacagcuacg gcuacaaguc cuucaucgag     2220

gaggagaaca agaaguacua caugaucaac ccgaccggga gcaucgccca gacgcuccgg     2280

gagauguacg ugucccccag cgucagccgc gccaucaucc ggaccaugga gaucgugaag     2340

gagcugcgca agaucaccaa gaaggacccc gacaagaucu ucguggagau ggcccggggc     2400

ggcaagcccg aggagaaggg gaagcgcacc uccagccgcc gggagcagau cgagaagcug     2460

uacgacuccg cgaaggccuu cgucagcgac gaggacaucu cccacaugcg cagccagcuc     2520

ggcucccuga gcgacgagca gcugcggcgc gagaaguacu accucuacuu cauccaguuc     2580

gggaagugca uguacuccgg cgaggccauc gacuucagcc ggcuggggga caaccacugc     2640

uacgacaucg accacaucuu cccgcagucc aagaucaacg acgacagccu gcacaacaag     2700

gugcucguga agucccagcu gaacggcgag aaguccgacg acuaccccau caaggccgag     2760

auccgcaaca agaugcaccc ccuguggaag aaccucuucu accgggaccc caagaacccg     2820

gccgacaagg ucaaguacga gcgccugacc cggagcacgc ccuucaccga ggacgagcug     2880

gcgggcuuca ucgagcgcca gcucguggag acccggcaga gcaccaaggc cguggccacg     2940

cugcugaagg agauguuccc cgacuccaag aucgucuacg ugaaggccgg ccaggugagc     3000

gaguuccgcc acgacuucga caugcucaag ugccgggaga ucaacgaccu gcaccacgcc     3060

aaggacgcgu accugaacau cgucaucggg aacgugcacg acgugaaguu caccuccaac     3120

ccgaugaacu ucgucaagaa cgccgacaag cacuacacca ucaaggugaa ggagacccuc     3180

aagcacaagg ugagccgcaa cggggagacg gccuggaacc ccgagaccga cuucgacacc     3240

gucaagcgga ugaugagcaa gaacuccgug cgcuacgugc gguacugcua caagcgcaag     3300

ggcggcuucu ucaaccagaa ccccgaccgg gccgggaacc ccgaccuggc ccgccugaag     3360

aaggaccucg acccggucaa guacggcggg uacaacagca aguccaucag cugcuucucc     3420

cugaucaagu gcaccggcgu gggggcggug aucauccccg ucgagcugcu cugcgagaag     3480

cgguacuuca gcgacgacuc cuucgccagc gaguacgccu acuccgugcu gaagaacgcc     3540

cugcccgcga agaacaucgc caagaucagc aucgacgaca ucuccuuccc ccucaagcgc     3600

cgcccgauca agaucaacac gcuguucgag uucgacggcu accgggugaa cauccgcagc     3660

aaggacuccu accacuacuu caccaucagc uccgccaugg ccgccaucua cagcaaggac     3720

accuccgacu acaucaaggc ggucagcucc uacaucgaca agagcgacaa gggcagcaag     3780

uucaagcccg gggaggccuu cgacgugcug uccaaccuca agguguacga cgagaucgcc     3840

aagaagugca ucagcgagcc cuucuccaag aucagcaagc uggccgaggc cggcaagcgg     3900

uggaagaagg aggagaucuc ccugcagaac ugccuccugu ga                        3942


<210>  1766
<211>  3945
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1766
augcugaaga aggacuacag caucggccuc gacaucggga ccaacuccgu gggccacgcc       60

gucgugaccg acgacuacaa ggugcccacg aagaagauga aggucuucgg ggacaccagc      120

aagaagacca ucaagaagaa caugcugggc gugcuccucu ucaacgaggg gcagaccgcc      180

gcggacacgc gccugaagcg gggcgcccgc cggcgcuaca cccggcgcaa gaaccggcug      240

cgcuaccucc aggagaucuu cgccccggcc cuggccaagg uggaccccaa cuucuucuac      300

cggcuggagg aguccagccu cgucgcggag gacaagaagu acgacgugua ccccaucuuc      360

ggcaagcgcg aggaggagcu gcuguaccac gacacccaca agaccaucua ccaccuccgg      420

uccgagcugg ccaacaacga ccgccccgcc gaccugcggc ucguguaccu ggcccuggcg      480

cacaucauca aguaccgcgg gaacuuccuc cuggagggcg agaucgaccu gcggacgacc      540

gacaucaaca aggucuucgc cgaguucagc gagacccuca acgagaacuc cgacgagaac      600

cuggggaagc uggacguggc cgacaucuuc aaggacaaca ccuucagcaa gacgaagaag      660

uccgaggagc uccugaagcu gagcggcgcc aagaagaacc agcucgccca ccagcuguuc      720

aagaugaugg uggggaacau gggcuccuuc aagaaggucc ugggcaccga cgaggagcac      780

aagcucagcu ucgggaagga caccuacgag gacgaccuga acgaccugcu cgcggaggcc      840

ggcgaccagu accuggacau cuucguggcc gccaagaagg uguacgacgc cgcgauccug      900

gccuccaucc ucgacgucaa ggacacccag acgaagaccg uguucagcca ggccaugauc      960

gagcgcuacg aggagcacca gaaggaccug aucgagcuga agcggguguu caagaaguac     1020

cucccggaga agugccacga cuucuucucc gagcccaaga ucagcgggua cgccggcuac     1080

aucgacggca aggucuccga ggaggacuuc uacaaguaca ccaagaagac ccugaagggg     1140

auccccgaga cggaggagau ccugcagaag aucgacgcca acaacuaccu ccgcaagcag     1200

cggaccuucg acaacggcgc gaucccccac caggugcacc ugaaggagcu gguggccauc     1260

gucgagaacc aggggaagua cuacccguuc cuccgcgaga acaaggacaa guucgagaag     1320

auccugaacu uccggauccc cuacuacgug ggcccccugg cccgcgggaa cagcaaguuc     1380

gccuggcuca cccgcgccgg cgagggcaag aucacccccu acaacuucga cgagaugauc     1440

gacaaggaga cguccgcgga ggacuucauc aagcggauga ccaucaacga ccuguaccug     1500

ccgaccgagc ccgugcugcc caagcacagc cuccuguacg agcgcuacac caucuucaac     1560

gagcuggccg ggguccggua cgugacggag aacggcgagg ccaaguacuu cgacgcccag     1620

accaagcgca gcaucuucga gcuguucaag cuggaccgga aggucuccga gaagauggug     1680

aucaagcacc ugaagguggu caugcccgcg auccgcaucc aggcccucaa ggggcuggac     1740

aacggcaagu ucaacgccag cuacgggacc uacaaggacc ugguggacau gggcguggcc     1800

ccggagcucc ugaacgacga ggucaacucc gagaaguggg aggacaucau caagacccug     1860

acgaucuucg agggccggaa gcucaucaag cgccggcugg agaacuaccg cgacuuccug     1920

ggggaggaca uccuccggaa gcugagccgc aagaaguaca ccggcugggg gcggcugucc     1980

gccaagcucc uggacggcau cuacgacaag aagacccaca agaccauccu ggacugccuc     2040

augacggagg acuacagcca gaacuucaug cagcugauca acgacgacac cuacuccuuc     2100

aaggagacca ucaagaacgc ccaggugauc gagaaggagg agacccuggc caagaccgug     2160

caggagcucc ccgggagccc cgccaucaag aagggcaucc ugcagucccu ggagaucguc     2220

gacgagauca ucaaggugau gggcuacaag cccaagagca ucguggucga gauggcccgc     2280

gagacgcaga agacccacgg gacccggaag cgcgaggacc gggugcagca gaucgugaag     2340

aaccucaagg acgccaacga gcugccgaag aagcugcccu ccaacgcgga gcucagcgac     2400

gagcgcaagu accuguacug ccugcagaac ggccgggaca uguacaccgg ggcgccccuc     2460

gacuacgacc accuccaguu cuacgacguc gaccacauca ucccccaguc cuuccugaag     2520

gacgacagca ucgagaacaa ggugcugacg aucaagaagg agaacgugcg caagaccaac     2580

ggccugccgu ccgaggccgu cauccagaag augggcagcu ucuggaagaa gcugcucgac     2640

gccggggcca ugaccaacaa gaaguacgac aaccugcgcc ggaaccugca cggcgggcuc     2700

aacgagaagc ugaaggagcg cuucaucgag cggcagcugg uggagacccg ccagaucacg     2760

aaguacgugg cgcagcuccu ggaccagcgg cugaacuacg acggcaacgg ggucgagcuc     2820

gacgagaaga ucgccaucgu gacccugaag gcccagcugg ccucccaguu ccgcagcgag     2880

uucaagcucc ggaaggugcg cgcgcugaac aaccugcacc acgcccacga cgccuaccuc     2940

aacgccgucg uggccaaccu gaucauggcg aaguaccccg agcuggagcc cgaguucgug     3000

uacggcaagu accggaagac caaguucaag ggccucggga aggccaccgc caagaacacg     3060

cuguacgcca acguccugua cuuccucaag gagaacgagg uguaccccuu cugggacaag     3120

gcccgcgacc ugccgaccau caagcgguac cuguaccgcg cccaggugaa caagguccgg     3180

aaggcggagc gccagaccgg cggguucucc gacgagaugc ucgugcccaa gagcgacagc     3240

ggcaagcugc ugccccggaa ggaggggcuc gacccgguga aguacggcgg cuacgccaag     3300

gccgucgagu ccuacgccgu gcugaucacc gcggacgagg ugaagaaggg gaagacgaag     3360

aaggucaaga cccuggugaa cauccccauc aucgacagca agaaguacga ggccgacccc     3420

accgccuacc ucgccucccg cggcuacacc aacgugacga acagcuucau ccugcccaag     3480

uacucccugc ucgaggaccc ggaggggcgg cgccgguacc uggcgagcuu caaggaguuc     3540

cagaaggcca acgagcugau ccucccccag caccuggucg agcugcucua cugggugaac     3600

gccaaggacg gcgagcagaa gcuggaggac cacaaggccg aguucaagga gcuguucgac     3660

aagaucaugg aguucgccga caaguacgug gucgcgccca agaacuccga gaagauccgc     3720

cggcucuacg aggagaacca ggacgccacc cccauggagc uggggaagaa cuucguggag     3780

cugcuccgcu acaccgccga cggcgccgcc agcgacuuca aguucuucgg cgagaacauc     3840

ccgcgcaagc gguacaacuc cgcggggagc cugcugaacg gcacccucau cuaccagucc     3900

aagacggggc uguacgagac ccgcaucgac cugggcaagc ucuga                     3945


<210>  1767
<211>  3948
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1767
augggcuaca gcguggggcu ggacaucggc accggguccg ucggcugggu ggugaccaac       60

gaggacggga agcucgcccg cgccaagggc aagaaccuga ucggcguccg gcuguucaag      120

acggcggagc ccgccgccga ccgccggacc aaccgcacca cccggcgccg gcucagccgc      180

cggaaguggc gccugcggcu gcucgacgag cuguucgcca acgagcuggg gaagaucgac      240

uccaacuucc ucacgcgccu gaaguacagc uacgugcacc cgaaggacga cgccaaccgg      300

gcgaacuacu acggcgggua ccuguucccc acccaggagg agaccaagaa guuccacgcc      360

aaguacccca ccaucuacca ccuccgccug gcccugauga cggagucccg gcaguucgac      420

cuccgcgaga ucuaccuggc caugcaccag aucgugaagu accggggcca cuuccugaac      480

agccagucca agaugaccau cgggaacacc uacaaccccc aggaccucca gaccgcgaug      540

gagaacuacg ccgaggccaa gggccugagc aucgagcuga agcagccgga ggagaugguc      600

gccauccuca cgggcgaggc cggguucggc cuguccaaga aggcgaaggc cgagaagcug      660

cucaacaacu ucuucuucga caccaaggag gagaaggcca ccgugcaggc cauccuggcc      720

gggaucgugg gcaacaccau cgacuucacg aaggucuuca accgccagcu gaccggcgac      780

acccucaagg cguggaagcu gaagcuggac agcgagaccu ucgacgugga cuccgagccc      840

auccucagcg accuggacga cgacgagacg gagcuguucu ucgccauccg gcaggccuuc      900

gacggguuca cccucaugaa ccugcugggc gaccagaccu ccaucagcgc cgccauggug      960

gagcgcuaca cccagcacca cgacgaccuc cugacgguca agcaggucgc gaagaagcag     1020

gggcuguccc acaaggaguu cagcaacauc uacuccgccu uccucaagga cgacaccgac     1080

aagggcauga agaagcugcu ggaggagagc aaccucucca ccgaggacgu gaacgggauc     1140

cagcugcgga uggacagcca cgacuuccug ccccagcagc gcaccaaggc caacagcgug     1200

aucccccacc agcuccaccu ggccgagcug gaggccauca ucgagaacca gggccaguac     1260

uacccguucc uccuggagac guucgagaag gagggcaccg cggccaacaa gaucgccgag     1320

cugcugaagu uccgcauccc cuacuacgug gggcccaugg ugaccaagaa ggacgucgag     1380

aacgccggcg gggacgcgac caaccacugg gugacgcgga acgagggcua cgagaagucc     1440

gccgugaccg ccuggaacuu cgaccagguc uucaaccgcg accaggccgc ccucgacuuc     1500

auccagcggc ugaccgggac cgacacguac cugaucggcg agcccacccu cccgcagaac     1560

agccugaagu accagauguu caccgugcug aacgagcuca acaacgugaa ggucaacggc     1620

cgccggaucg acaaccagac caagcacgac cugauccagg accuguucaa guccaagaag     1680

accgugagcc ucaaggcguu ccaggacuac uaccaguccc agggcaagag cgacauccag     1740

gucguggggc uggccgacaa gacgaaguuc aacuccaacc ugagcaccua caucgaccuc     1800

uccaagaccu ucagcgccga ggagauggag aacccccaga accaggaccu gcuggagcag     1860

aucguggaga uccagaccgu cuucgaggac uccaagaucg ccgagcgcga gcucagcaag     1920

cuggagcuga cgcccgagca ggucaccaag cucgccaaga cccacuacac cggguggggc     1980

aaccugucca acaagcugcu cacgaccccc aucguggacg ugagcgggaa gaaggugucc     2040

auccuggacc agcuggcgaa gaccagcaag aacuucaugu ccaucaucag caacgagcag     2100

uacggcgugc aggacuggau caaggagcag aacaccacgg agaccaccgg cacccucgag     2160

gacaagcugg ccgaccugac gaccuccccg gccaacaagc gggggaucaa gcaggccuuc     2220

aacguccucu ucgacaucca gaaggccaug aaguccgagc ccgaccgcgu guaccuggag     2280

uucgcgcggg agacccagac cagcgugcgc acgaugagcc gguacaacua ccugaagggc     2340

cucuaccaga gcaagucccu ggacgccgag gccaagaacc ugaagaagga gcucgccgag     2400

accgaccaga agaaguucga caacgaccgc cuguaccugu acuuccagca gcaggggaag     2460

gacauguaca ccggccagcc caucaacauc gacaagcuca gcuccgacua cgacaucgac     2520

cacaucaucc cccaggcguu caccaaggac gacagcaucg acaaccgggu ccuggugucc     2580

cgcccggaga acgcccgcaa gagcgacucc ggcacguaca ccaccgaggu gcaggccgcc     2640

gccagcgggc uguggaagac ccucguccag uccggcuuca ucagccggaa gaaguacgac     2700

cggcugacgc acgcggggga cuacuccgag ggccagaaga ccggguucau cgcccgccag     2760

cugguggaga cccggcagau caucaagaac guggccgccc ucaucgagag cgaguucgac     2820

cagacccagg ccgucgcgau ccgcgccgag aucacgggcg acaugcgccg gcugguggcc     2880

aucaagaagc accgcgagau caacuccuuc caccacgccu ucgacgcccu gcucaucacc     2940

gcggccggcc aguacaugca gaagcgguac cccgaccgcg acagcaacaa cguguacaac     3000

gaguucgacc gguacaccaa cgacuaccug aagaagcugc gcgaguccag cagcaaggac     3060

gagguccgga agcucaagcc cuucggguuc aucgugggca ccauggccaa ggggaacgag     3120

gacuggacga ccgagcagac cgaguaccug cgccacauca ugaacuucaa gaacauccug     3180

accaccuaca agacgggccc cgaccggggg ccgcucucca aggagaccau cuacgccgug     3240

gaccccaagg cgaagcugau cggcaccaac aagcaccgcc aggacgucag ccuguacggc     3300

ggguacaccu accccuacuc cgccuacaug acgcucgugc gggccaacgg caagaaccag     3360

cuggugaagg ucagccgcuc cgccgccagc cugaugaagu ccgggcagcu caagcuggag     3420

gacuacgugc agcagcagcc cgaggugaag aaguucgaga agauccugcu cgagcaccuc     3480

ccgcugggcc agcuggucga caacgacggg aagcugaugu accugaccag cuacgaguuc     3540

uaccacaacg cgaagcagcu cuggcugucc accgagaacg ccgaccugau cagcucccuc     3600

agcaucgacu ccgacgacca gaagcugauc aaggccuucg acauccugac cgccgacagc     3660

gugaucgagc gguuccccuu cuacgagauc gaccucaaga agcugaucga gcugcgcgac     3720

aaguucgugc ucaucgacaa caagcuggac guccugcggg uggugcucaa ggcccugcag     3780

cuggacuccg cgcagcagaa gccccucaag cugaucgaca aggccgccac ggacuggaag     3840

aacuaccgcc aggccggcgg caucaagcug agcgacaacg ccgagaucau cuaccagucc     3900

accaccggga ucuucgagaa gcggaucaag aucagcgacc uccuguga                  3948


<210>  1768
<211>  3951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1768
augcugcaga agaacuacac caucggccuc gacaucggga ccaacagcgu gggcuacgcc       60

gucaucaacg acaacuacaa ggugcccgcc aagaagauga aggugcuggg gaacacggac      120

aagaagacca ucaagaagaa ccugaugggc guccuccugu ucgacgcggg ggagaccgcc      180

gccaccacgc gccugaagcg gggcacccgc cggcgcuaca cccggcgccg gaaccgccuc      240

cgguaccugc aggagauccu gggcccggag cucgccaagg uggacgccaa cuuccugcac      300

cgccuggcgg aguccagccu cgugaccgag gacaaggagu ucuccgccua ccccaucuuc      360

ggggaccggg aggaggagcu gcuguaccac aagacguaca agaccaucua ccaccuccgc      420

agcgcccugg ccaacaagga cgagaaggcg gaccugcggc ucaucuaccu ggcccuggcc      480

cacaucauca aguaccgcgg caacuuccuc aucgaggggg agaucgagcu guccgacacc      540

gacaucgaca aggucuacgc cgacuucugc cggagcuuca acgagcuguu cgacaccgag      600

gugcuccagg agaucgaggu ggaggccauc uucaaggaca agacgcuguc caagaccaag      660

aaggcggagg agcuggucaa ggugagcggc gugaagaaga accaggucgc ccaccaguuc      720

cucaagauga ugguggggaa caccgugaac uucaagaagg ucuucaaccu gaccgaggac      780

cagaagcugu ccuucggcac ggacagcuac gaggacgagc ucaacgaccu gcuggccaag      840

uccgacgacc gcuaccucga cguguucgug gccgccaaga aggucuacga caccgcgauc      900

cuggccaaca uccugaacac caagaacagc guggccacca agacgaucuu cucccagagc      960

augaucgagc gguucaagga ccaccagaag gaccucaagg agcugaagca gcuguucaag     1020

accuaccucc ccgagaagua ccccgacuuc uucggcaacu ccaaggagga cggguacgcc     1080

ggcuacaucg aggggaagac caacgaggag aaguucuaca cccacacgaa gaagauccug     1140

aagaacaucc cgcaggccgc gagcgugcug gcccagaucg acgucggcaa cuaccuccgc     1200

aagcagcgga ccuucgacaa cggcgugauc ccccaccaga uccagcugaa ggagcugcuc     1260

gccaucaucg agaaccaggg gaaguacuac cccuuccugc uggagaacaa ggagaagauc     1320

gccaagaucc ucaccuuccg cauccccuac uacgugggcc cgcuggcccg ggggaacucc     1380

cgcuucgcgu ggcugaagcg cgucggcaag gagaagauca cccccuucaa cuucgagaac     1440

guggucgacc uggagagcuc cgccgagcgg uucaucgagc gcaugacgcu caacgaccug     1500

uaccugcccg aggagaaggu gcuccccaag cacagcaugc uguacgagaa guucagcauc     1560

uucaacgagc uggccgggau ccgguacauc accgacaacg gcgagaugaa guuccucgac     1620

gcccagacca agcaguccau cuucgagcug uucaagcagg agcgcaccgu gaaggagaag     1680

aagguccugg accuccugaa gacgcugaug ccgagcgagc gggugguggc gaucaagggc     1740

aucgacaacg ggaaguucaa cgccuccuuc ggcaccuacc acgaccucag caagaugggg     1800

uucaagaccg agaagaucgc cuccgagagc aaccaggccc gcugggagga gaucaucaag     1860

acccugacga ucuucgagga ccggaagcug aucaaguccc gccuccggaa guacgacgac     1920

uuccugaacg agaaggagau cggcgcccug agcaagcgca aguacaccgg guggggccgg     1980

cucuccgcga agcugaucga cggcaucuac gacaagaaga cccaccagac cauccuggac     2040

uuccucauga acgaggacua caaccagaac uucaugcagc ugaucagcaa cgacaacuac     2100

uccuucaaga agaucaucga ggacagccag gucaucaagg aggagggguc ccugaccgag     2160

gugauccagg agcuccccgg cagccccgcc aucaagaagg ggauccugca guccaucgag     2220

aucguggacg agcuggucaa ggugaugggc gggaacccca agaagaucgu ggucgagaug     2280

gcccgcgaga cgcagaagac ccacggcacc acccggcgcg aggcccggau caaggagauc     2340

aucaagaacc ucaacaagcu gaagcugaac gagcucccga aggaccugcc cagcaacgag     2400

gagcuguccg acgagaaggu guaccucuac ugccugcaga acggccgcga cauguacacg     2460

ggggcgcccc uggaccucga caaccugagc caguacgagg uggaccacau caucccccag     2520

uccuuccuga aggacgacag caucgagaac aagguccuca ccgccaagau cgagaacagc     2580

cggaagacca acggccugcc guccguggag aucgccaaga agaugggggc cuucuggcgc     2640

agccugcuca aggugggcgc caucuccgag aagaaguacc ggaaccugcg ccgcagccug     2700

cacggcggga ugaccgagaa gcucaaggag acguucguca agcggcagcu gguggagacc     2760

cgccagauca ccaaguacgu ggcccagauc cugaacuccc gguucaacga ggagaagaac     2820

gaggacaagg ucgcgaucgu gacccucaag gcccagcuga cgagccaguu ccgcaaggac     2880

uucgagcugu acaaggugcg ggagaucaac aaccuccacc acgcccacga cgccuaccug     2940

aacgcggucc uggccacccu ccugaugacc uccuuccccg agcuggagcc cgaguucgug     3000

uacggcaagu accgcaagac caaguucaag gggcucggca aggccacggc caagaacaac     3060

cuguacacca acauccugca gcuccugaag gaggagagcg cccaguacug ggacaccaag     3120

gagcgggacc uggcgaccau caagaagaac guguaccagg cccaggucaa caaggugcgc     3180

aagguggagc ggcagacggg guccuucuac gacgagaccg ucgccaagaa guccgccagc     3240

cagaagcugu ucccccgcaa gaacggccug gacccgguga aguacggcgg guaccagcgg     3300

gccaacgagg gcuacagccu ccucuuccgc gcggacaugc ugaacaagcg ggggaagacc     3360

aaguccguga agacccuggu caagaucccc aucaucgaga gcgcccagua ccucgccgac     3420

cccacggagu accuggccaa gcgcggcuac uacaacgugu ccgagcacag ccugcuccug     3480

cccaaguucu cccugcucga gaacaacaac gggcugcggc gcuaccuggc cagcagcaac     3540

gaguuccaga aggcgaacga gcucauccug ccgaagcacc uggugcggcu ccuguacugg     3600

ucccagagca agcagcgcaa ggaggagcug aacaagcacc gggccgaguu cgccgagcug     3660

uucaccuaca ucauggacuu cgccgacaag uacgucgugg cgcccaagaa cuccgagaag     3720

aucaagaaca ucuacgagaa caacaaggac agcuccgugg agguccucgg caagaacuuc     3780

aucgagcugc ucaaguacac cgccgacggc gccgccagcg acuucaccuu cuucgacacg     3840

aagauccccc gcaagcggua caacuccgcc ggggaccugc uggacggcac ccucaucuac     3900

cagagcaaga ccgggcugua cgagacccgc aucgaccugg gcgcgaucug a              3951


<210>  1769
<211>  3957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1769
augaugauca agcugaacaa caugaagaag auccucggcc uggaccuggg gaccaacagc       60

aucggcuggg ccguggucga gaccgaggac aacaagaagu ucgagcuccu ggacaagggg      120

gugcgcaucu uccaggaggg cgugaagauc gagaagggga ucgaguccag caaggccgcg      180

gagcggacgg gcuaccgcuc cgcccggcgc aucaaguucc ggcgccggcu gcgcaagauc      240

gagacccuca agguccugau cgagaacaac augugccccc ugagcaugga cgagcucauc      300

guguggaagg agaacaagaa caacuacccg aagaacaaga ucuucuacaa cuggcugaag      360

accaaggacc ccaaggaggg caccaacggg acguacauca accccuacua cuuccggacc      420

gaggccauca agcgcaagcu ggagaacacc aacgacgugg gccgggcccu cuaccaccug      480

gcccagcgcc ggggguucaa guccaaccgc cuggagagca ccgacgagaa gucccggggc      540

aucgucaaga cggagaucaa caagcucagc uccgagcuga acgggcgcua ccugggcgag      600

uacuucuacg agcucuaccu gaagggcgag aagauccgga agcgccacau cagccgggag      660

gagcacuacc ugaacgaguu caagaagauc ugcgaggugc agaacuucaa caaggagcuc      720

gcggagaagc uggagcgcgc caucuucuac cagcggcccc ugaaguccca gaaggggcuc      780

gugggcaagu gcacccugca gaacaacaag caccgcugcc cgaucagccg ccccgaguuc      840

gaggaguucc ggauguggca guucaucaac uccaucaagu acaagaacag cgacgggaag      900

uucgacuucc ugaacaagga gcagcgcgag aacaucaucc ccgccuucgu cuccaacaag      960

aacgccaccu ucgaguucag caagaucgag aagauccuca acaagggcgu gcacaucuac     1020

caguucaacu acaaguccaa ccacaccauc ggcagcaacc agacgaugau caacuucaug     1080

gacguguucg acaucgaccg gaagcugacc aacgcguggc ugcucugcgu caacaagauc     1140

uacgagcugu acaccgccaa gaccaagaag gacggguccc ucaagagcaa gagcgaggug     1200

aucacggacg uguggcacgu ccuguucacc uucgacaacg aggagaaccu gaaggaguuc     1260

gcccgcaaca agcugcuccu ggacgagucc agcgcccaga aguucuccaa gaucaaccug     1320

aagcaggacu acagccagcu cucccugaag gccaucaaca agauccugcc cuaccuccag     1380

gagggccuga ucuacagcua cgcgguguuc aucgccaaca ucgagaacgu gaucgggaag     1440

uccaucugga acaucaagga gaaccagcag aucauccgga ccagcaucaa gaagaucauc     1500

gacaaccaca agaccgagcu ggacaucaac acgaucacca acucccucau caacgacuuc     1560

aaggucaacu acaacaacgc ccacaaggag uacaaggugg acgacaucga ccugaagaac     1620

auccgcgaga agaucaucaa guacuacggc aacaagaccu ggaacugccu gccggaggac     1680

gagaagcucu acgugaagga gaaggucgag aagcuguacg agaagcagcu caagagcggg     1740

aagcggggca ucuucaucga gaagauccgc cuggacgacc ggaucgagaa cuuccugaag     1800

gacaacuucg acuccagcga gcugaaccug aagaagcucu accaccccuc cgacgugagc     1860

guguacaagu ccgucaagau caccgaccug ggccugcgcc uccuggggag ccccaugauc     1920

cccuccauca agaacccgau ggccaugcgg gccaugcacc agcugcgcaa gcucaucaac     1980

caccugauca ucgagggcgu gaucgacgac agcacgauca ucaacgucga gaugucccgg     2040

gagcugaacg acgcgaacau gcgcaaggcc aucgagcggu accagcgcca gcgggccacc     2100

gagaacgagg aguucaagac caagaucaag gagcucuaca agaaggagug cgggaccgac     2160

uucgagccca acaacgacga caucaugaag uaccgccugu gggaggagca gaaccacaag     2220

ugccuguaca cgggcaagag caucgggauc ugcgacuuca ucggcagcaa ccccgccuuc     2280

gagaucgagc acaccauccc ccggucccuc agcuacgaca acucccugga gaacaagacc     2340

cugugcgaga aggaguucaa ccagcagguc aagaagaacc gcaucccguu cgagcucagc     2400

aacuacaagg agaucaucuc ccgcaucaac aacugggaga ucaaggccga ggagcuguuc     2460

aagcagaucc agaaggugaa gauccagagc cggauggcgg ccaccaagga gaucaaggac     2520

aacgccaucc agcgcaagca ccuccugcag auggaguuca acuacuggaa cggcaagcug     2580

ucccgguuca cgcgcgagga cgugcccgag ggguucaaga acagccagcu gguggacauc     2640

ggcaucauca ccaaguacgc ccgggccuac cucaagaccg uguucaacaa cgucaacacc     2700

gugaaggggu ccacgguggc ggaguuccgc aacaucuggg gccugcagga guacuucgag     2760

aagaaggacc ggagcaacca cauccaccac ugcaucgacg ccaucaccau cgccugccug     2820

accaaggaca aguacgacuu ccucgcccac cuguaccacc uggacgagca gaacaaccgc     2880

gacgcggucc accgggccau gaucaagucc aagcccuggg agaacuucgu gcaggacgug     2940

aaggagaucg agaaccagau ccucgucagc cacuacaccc ccgacaaccu gccgaagcag     3000

uccaagaaga uccugcgcaa gcgggggaag auccagaaga acgagaacgg cgaccucauc     3060

uacgagaagg gcgacgccgu gcgcgggagc cugcacaagg aguccuucua cggcgccauc     3120

aagcgggaga ucaucaacaa gaagggggag accgaggaga aggugcugua cgucaagcgc     3180

aagccccucg aguucagcga ccugggcuuc aagacgaucu ccgagcugga gaacaucgug     3240

gacgacgccg ugcgggacaa gguccucaac gcggugaagg acaagggccu gaagcaggcc     3300

cugaacgagc ccaucuggau gaacgaggag aagcagaucc ccaucaagaa ggugcgcugc     3360

uacaccccga gcgucaccaa ccccaucaac cucaagaagc accgggacaa gagcaagcac     3420

gaguacaagc aguccuacca cgugaccaac gacgggaacu accugauggu gaucuacgag     3480

ggcaaggaca gccaggagaa ggucaagcgc gacuacgaga ucguguccaa ccugcaggcc     3540

ggggaguacu ucaagcagag cgugcacggc guccucaagg cccaggggau caacaaccug     3600

gagggccuga uccccaaguu caaggagucc ggcaagaaca uccucccccu gaaguacauc     3660

cugaagacgg ggaccauggu gaucuucugg gagaacuacc cggaggagau cugggacauc     3720

gacaagaacc agcucuacaa gcggcuguuc aaggugaccc aguucgagag cgacggccgc     3780

auccaggccc gguuccacca gaucgcgaag cccgacaacg agcugaccaa ggucuccgag     3840

cucgacuuca gcaacaucgc cgagaagauc cgccugucca aggggaaccu gaacaugcuc     3900

gugaacggcu acgacuucaa gaucaacgug cuggggcaca ucgagaagcu gagcuga        3957


<210>  1770
<211>  3957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1770
augggcagca acaugcugaa gaaggacuac uccaucgggc ucgacaucgg caccaacagc       60

guggggcacg ccgucgugac cgacgacuac aaggugccca cgaagaagau gaaggucuuc      120

ggcgacaccu ccaagaagac caucaagaag aacaugcugg gggugcugcu guucaacgag      180

ggccagaccg ccgcggacac gcgccugaag cggggcaccc gccggcgcua cacccggcgc      240

aagaaccggc ugcgcuaccu ccaggagauc uucgccccgg agcuggccaa gguggacccc      300

aacuuccugu accggcucga ggagagcucc cuggucgccg aggacaagaa guacgacgug      360

uaccccaucu ucgggaagcg cgaggaggag cugcucuacc acgacaccua caagacgauc      420

uaccaccucc ggagcgcccu ggcgaacaac gaccagcccg ccgaccuccg ccugguguac      480

cuggcccucg cccacaucau caaguaccgg ggcaacuucc ugcuggaggg ggagaucgac      540

cuccgcacca ccgacaucaa caaggucuuc gcggaguucu ccgagacccu gaacgagaac      600

agcgacgaga accugggcaa gcucgacgug gccgacaucu ucaagaacaa cacguucucc      660

aagaccaaga aguccgagga gcugcugaag cucagcgggg ccaagaagaa ccagcuggcc      720

caccagcugu ucaagaugau ggugggcaac augggcagcu ucaagaaggu ccucgggacc      780

gacgaggagc acaagcuguc cuucggcaag gacaccuacg aggacgaccu gaacgaccuc      840

cuggccgagg cgggggacca guaccuggac aucuucgugg ccgccaagaa gguguacgac      900

gccgccaucc ucgcgagcau ccuggacguc aaggacacgc agaccaagac cguguucucc      960

caggccauga ucgagcggua cgaggagcac cagaaggacc ugaucgagcu caagcgcgug     1020

uucaagaagu accugccgga gaagugccac gacuucuuca gcgagcccaa gaucuccggc     1080

uacgccggcu acaucgacgg gaaggucagc gaggaggacu ucuacaagua caccaagaag     1140

acgcugaagg gcauccccga gaccgaggag auccuccaga agaucgacgc caacaacuac     1200

cugcggaagc agcgcaccuu cgacaacggg gccauccccc accaggugca ccugaaggag     1260

cucguggcga ucgucgagaa ccagggcaag uacuacccgu uccugcggga gaacaaggac     1320

aaguucgaga agauccugaa cuuccgcauc cccuacuacg uggggccccu cgcccggggc     1380

aacuccaagu ucgccuggcu gacccgcgcc ggcgagggga agaucacgcc cuacaacuuc     1440

gacgagauga ucgacaagga gaccagcgcc gaggacuuca ucaagcacau gaccaucaac     1500

gaccuguacc ugccgaccga gcccgugcuc cccaagcaca gccugcugua cgagcgcuac     1560

acgaucuuca acgagcucgc gggcguccgg uacgugaccg agaacgggga ggccaaguac     1620

uucgacgccc agaccaagcg cuccaucuuc gagcuguuca agcuggaccg gaaggucagc     1680

gagaagaugg ugaucaagca ccucaaggug gucaugcccg ccauccgcau ccaggcgcug     1740

aagggccugg acaacgggaa guucaacgcc uccuacggca ccuacaagga ccucguggac     1800

augggcgugg ccccggagcu gcugaacgac gaggucaaca gcgagaagug ggaggacauc     1860

aucaagacgc ucaccaucuu cgaggggcgg aagcugauca agcgccggcu ggagaacuac     1920

cgcgacuucc ucggcgagga cauccugcgg aagcuguccc gcaagaagua caccgggugg     1980

ggccggcuca gcgccaagcu gcuggacggg aucuacgaca agaagaccca caagacgauc     2040

cucgacugcc ugaugaccga ggacuacucc cagaacuuca ugcagcugau caacgacgac     2100

aacuacagcu ucaaggagac caucaagaac gcccagguga ucgagaagga ggagacccuc     2160

gcgaagaccg ugcaggagcu gcccggcucc cccgccauca agaagggcau ccugcagagc     2220

cucgagaucg ucgacgagau caucaaggug augggguaca agcccaaguc caucgugguc     2280

gagauggccc gcgagacgca gaagacccac ggcacccgga agcgcgagga ccgggugcag     2340

cagaucgugg agaaccugaa ggacgccaac gagcugccgg agaagcuccc cagcaacgcc     2400

gagcuguccg acgagcgcaa guaccuguac ugccuccaga acgggcggga cauguacacc     2460

ggcgcgcccc uggacuacga ccaccuccag uucuacgacg ucgaccacau caucccccag     2520

agcuuccuca aggacgacuc caucgagaac aaggugcuga cgaucaagaa ggagaacgug     2580

cgcaagacca acggccugcc gagcgaggcc gucauccaga agaugggguc cuucuggaag     2640

aagcuccugg acgccggcgc caugaccaac aagaaguacg acaaccugcg gcgcaaccuc     2700

cacgggggcc ugaacgagaa gcugaaggag cgcuucaucg agcggcagcu cguggagacc     2760

cgccagauca cgaaguacgu ggcccagcug cuggaccagc ggcucaacua cgacgggaac     2820

ggcgucgagc uggacgagaa gaucgcgauc gugacccuga aggcccagcu cgccagccag     2880

uuccgcuccg aguucaagcu gcggaaggug cgcgcccuga acaaccucca ccacgcgcac     2940

gacgccuacc ugaacgccau cgucgccaac cugaucaugg ccaaguaccc cgagcucgag     3000

cccgaguucg uguacggcaa guaccggaag gcgaaguuca aggggcuggg caaggccacc     3060

gccaagaaca cccuguacgc caacgugcuc uacuuccuga aggagaacga ggucuacccc     3120

uucugggaca aggcccgcga ccugccgacg aucaagcggu accucuaccg cgcccaggug     3180

aacaaggugc ggaaggcgga gcgccagauc gggggcuuca gcgacgagau gcuggucccc     3240

aagagcgacu ccgggaagcu gcucccccgg aaggagggcc uggacccggu gaaguacggc     3300

ggguacgcca aggccgugga gagcuacgcc guccugauca ccgcggacga ggugaagaag     3360

ggcaagacca agaaggugaa gacccucguc aacaucccca ucaucgacuc caagaaguac     3420

gaggccgacc ccacggccua ccuggccagc cgcggguaca ccaacgugac caacuccuuc     3480

auccugccca aguacagccu ccuggaggac ccggagggcc ggcgccggua ccuggcgucc     3540

uucaaggagu uccagaaggc caacgagcuc auccugcccc agcaccuggu ggagcuccug     3600

uacuggguca acgccaagga cggggagcag aagcuggagg accacaaggc cgaguucaag     3660

gagcuguucg acaagaucau ggaguucgcc gacaaguacg ugguggcgcc caagaacagc     3720

gagaagaucc gccggcugua cgaggagaac caggacgcca cccccaugga gcugggcaag     3780

aacuucgucg agcuccugcg cuacacggcc gacggcgccg ccuccgacuu caaguucuuc     3840

ggggagaaca ucccgcggaa gcgcuacaac agcgcgggcu cccugcucaa cgggacccug     3900

aucuaccaga gcaagaccgg ccuguacgag acccgcaucg accucgggaa gcuguga        3957


<210>  1771
<211>  3960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1771
auguucagcc agcgcaacaa ccccuacugc gagcuccugu ucaagaucau cgugaccaac       60

aucgaggaga agaaccugga ggucaucaug ggcauccgga acuccgacau cuucuucauc      120

agcaacuuca ugugguacca gaagcugauc augaugauca agaacauccu cgggcuggac      180

cugggcacca acuccaucgg gugggcccuc aucgaggugg acgagaacaa caucccgauc      240

cgcaucaucg ccaugggcag ccggaucauc ccccugucca cgagcgacaa ggaggaguuc      300

cagcgcgggc uguccaucac caagaaccag aaccggaccg ugagccgcac ccagcggaag      360

ggcuacgacc gcaagcagcu ccggcgcucc gaccugaaga agauccugaa gaaguacaac      420

aucuucccca gcgaggagcu ccugaagcug cccaugcucg accuguggaa gcugcggucc      480

gacagcgcga acccgaacga ggacaucucc gccgagcagc ucggccgcau ccuguacaug      540

cugaaccaga agcgggggua caagagcgcc cgcuccgagg ccaacagcga caagaaggac      600

acggacuacg uccaggccgu gaagggccgg uacgcgcagc ucaaggaccg ccggcagacc      660

aucgggcagu acuucuacga ggagcugucc accgccuuca gcgacaagaa caaccagcag      720

uacuuccgcg ugaaggagga ggucuacccc cgggaggccu acaucgagga guucgacacc      780

aucaucaacg cccagaagga caagcacggc uuccugacgg acaaggugau ccaccagcuc      840

cgcaacgaga ucaucuacuu ccagcggaag cugaaguccc agaaggggcu ggugagcauc      900

ugcgagcucg agggcuucga gaagaccguc uacgacaagg agaagaacaa gaacaaggug      960

guguucgucg gccccaaggu ggcgcccaag accagcccgc uguuccagcu gugccgcauc     1020

ugggaggugg ucaacaccau cucccucaag aucaagaacc ccgaggggag caaguacaag     1080

uggggcgaga aggugcccaa ccugaagcag aaggagaucc uggccgacua ccucuucaag     1140

aacgagaacc uguccuucag cacgcugcuc gagauccugg agcugaagaa ggaggacgug     1200

uacgucaaca agcagauccu caaggggauc aagggcaacg aggccuacgc cgcccugcac     1260

aacgugcugg gcgaccacga gcuccugaac uucgacgugu ccaucaucac cagcgaccac     1320

accgcgaucc uggucgacaa gaagaccggg gagauccucg aggagcgcgc cggccuggag     1380

cuggacgccu cccucgagcg ggagccccug uaccaguucu ggcacacgau cuacagccug     1440

aaggaccugg acgagugcaa gaacgcccuc aucaagcgcu ucgggcugga cgaggacauc     1500

gccgagaagc ugucccacau cgacuucaac aagcaggcgu ucggcagcaa guccaacaag     1560

agcaugcgga agauccuccc guuccugaug gagggguaca acuacuccga gagcugcucc     1620

cuggccggcu acaaccacag caacucccuc accaaggacg agcgcgagag ccagaucacc     1680

cuggagcggc uggagcuccu gcccaagaac aaccugcgcc agcccaucgu ggagaagauc     1740

cucaaccaga ugaucaacgu ggucaacacc auccuggaga aguacggcaa gcccuccgag     1800

auccgggugg agcuggcccg cgagcucaag cagagcaagg acgagcggaa cgacacggac     1860

cugcagaaca gccgcaacaa gaagcugaac gaggagaucg ggaagcggcu cgccgagcug     1920

ggccugccgg ucaccaagcg cuacauccag aaguacaagu ucaucuuccc cucccucagc     1980

aagaaccuga aggacgccca cgugaacaac cagugcaucu acugcgggga gaccuucaac     2040

cugaccgagg cgcucaacgg cgacgccuac gacguggacc acaucguccc caaggcccug     2100

cuguucgacg acucccagac gaacaaggug cucgugcacc ggaagugcaa cagcaccaag     2160

accaaccaga ccgccuacga cuacaucacg gccaaggggg aggaggaggu ccgcaucuac     2220

uccgagcggg uggacgacug guucaagaag ggcaucauca gcuacuccaa gaugcagcgc     2280

cugaagguga gccacaagga guaccuggag cggaagaagc ucggcaagga gaccgagacc     2340

gacaagaagc ugugggagaa cuucaucgac cgccagcugc gggacaccca guacaucucc     2400

cgcaagagcc gcgagauccu ccagaagguc ugcaacaacg ugacgaccac cgagggcggg     2460

gugaccgcga agcugcggaa ccuguggggg ugggacgaca uccucaugaa ccugcagaug     2520

cccaaguaca aggagcuggg ccagacgauc accaaggagu ggaccuccga gcacgggaag     2580

cgcaagcacc agaaggagga gaucgagaac uggaccaagc gggacgacca ccgccaccac     2640

gccaucgacg cccucgucgu ggccugcacc aagcagggcu ucauccagcg gaucaacacg     2700

cugaacagcu ccgagaccaa ggaccugaug aucaaggagg uggaggaggc gaagaucgag     2760

uucaacgaga agaccacccu ccuggagaag uaccugaaga cgcagaagcc guucaccacc     2820

cgcgagguca ugaaggaggc cgacaagauc cucaucagcu ucaaggccgg caagaaggug     2880

gccaccauca cgaaguucaa ggccaccggg aagaacaagg agaccggcgu gaucgucccc     2940

cgcggggcgc ugcacgagca guccguguac ggcaagauca aggugaucga gaaggacaag     3000

ccccugaagu accucuucga gaaccccgac aagaucguca acagccggau ccgggaccug     3060

guggagaccc gccuguccga gaacgagaac aacagcaaga aggcccucag cacggugaag     3120

aagaacccga ucuucuucaa cgaggagaag aacgagaucc uggagaaggc cuccugcuac     3180

aacgacgcca ccguccugaa guacaagcuc cagaaccuga aggccaacca gguggacgac     3240

aucguggacg agcgggucaa guuccuggug aaggagcgcc ucacccaguu cggcagcaag     3300

gagaaggagg cguucaagga cauccugugg uucaaccagg agaagcagau ccccaucauc     3360

accgugcggc uguucgcccg ccccgacgcc aacaaccucc agaucaucaa gaaggacaag     3420

aacgggaagg acaucggcuu cguccugacg gggaacaacc accacaucgc caucuacgag     3480

gacaaggacu ccaagcuggu gcagcacagc ugcgccuucu ggcacgcggu ggagcggaag     3540

aaguacaaca uccccgccgu caucaagaac uccgccgacg uguggagccu ccugcuggac     3600

aaggagcucc cggugcuguu ccuggacaag cuccccgccg acgagcugaa ccugaaguuc     3660

uccaugcagc agaacgagau guucauccuc ggccugagcc aggagggguu cgacgaggcg     3720

aucaagaaca acgacaaguc ccugcucagc aagcaccugu accuggucug guccaucagc     3780

aacaacgacu acuacuuccg ccaccaccuc gagaccaaga acaccgagcu gaagaacauc     3840

cccaccgcca aggacuccaa gcgguacuuc cgcuucaaga gcgugggcgc ccuggugggc     3900

aucaacccga ucaagguccg gcucaaccac cugggggaga ucacgaagau cggcgaguga     3960


<210>  1772
<211>  3960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1772
augggcuaca acaucgggcu ggacaucggc accgggagcg ugggcugggc cgcccucacc       60

gacgagggga agcuggcgcg cgccaagggc aagaaccuga ucggcguccg ccucuucgac      120

uccgcccaga gcgccgccca gcggcggucc uaccgcacga cccggcgccg gcugagccgc      180

cggaaguggc gccugcggcu ccuggagaac aucuucuccg acgagauggg gaugaucgac      240

gagaacuucu ucgcgcgccu gaaguacagc uacgugcacc ccaaggacga ggugaacaac      300

gcccacuacu acggcgggua ccucuucccg acccagcagg agacccacga cuuccacgag      360

aaguuccaga cgaucuacca ccuccggcug aagcucauga ucgaggacug caaguucgac      420

cugcgcgaga ucuaccuggc caugcaccac aucgucaagu accggggcca cuuccucaac      480

ucccagagca agaugaccau cggggacucc uacaaccccc gcgacuucca gcaggccauc      540

cagaacuacg cggaggccaa gggccugauc uggagccuga acgacgccca ggagaugacc      600

gacgugcucg ugggccaggc cggguucggc cuguccaaga aggccaaggc ggagcggcug      660

cucagcgccu ucuccuucga caccaaggag gacaagaagg ccauccaggc cauccuggcc      720

gggaucgucg gcaacacgac cgacuucacc aagaucuuca accgcgagcg gagcggcgac      780

gagcugaaga aguggaagcu caagcuggac uccgaggcgu ucgacgagca gagccaggcc      840

aucguggacg agcuggacga cgacgagaug gagcuguuca acgccauccg ccaggccuuc      900

gacggguuca cccugaugga ccugcucggc gaccagacgu ccaucagcgc cgcgauggug      960

aagcgguacc agcagcacca cgacgaccug aagaugguca aggagaucgc caagaagcag     1020

gggcuguccc accaggacuu cagcaagauc uacaccgccu uccucaagga cgacaccgac     1080

aagggcauga aggcccugcu ggacaaggcc gaccucgcgg acgacgugcu gguggagauc     1140

cagcagcgca ucgagagcca cgacuuccug cccaagcagc gcaccaaggc caacuccguc     1200

auccccuacc agcuccaccu ggccgagcug gagaagauca ucgagaacca ggggaaguac     1260

uacccguucc uccuggacac guucaccaac aaggccggcg agaccaucaa caagcuggug     1320

gagcucguga aguuccgggu ccccuacuac gugggcccca uggugaccgc ggccgacguc     1380

gagaaggccg ggggcgacgc cacgaaccac ugggugaagc gcaacgaggg guacgagaag     1440

ucccccguga ccccguggaa cuucgaccag gucuucaacc gggaccaggc cgcgcaggac     1500

uucaucgacc gccugaccgg caccgacacg uaccugaucg gggagcccac ccuccugaag     1560

aacagccuga aguaccagcu guucaccgug cucaacgagc ugaacaacgu gaagaucaac     1620

ggccacaaga ucgacgagaa gaccaagcac guccugaucc aggaccucuu caagagcaag     1680

aagacggugu ccgagaaggc caucaaggac uacuaccuga gccagggcau gggggagauc     1740

cagaucgucg gccuggccga caagaccaag uucaacucca accucagcuc cuacaucgac     1800

cugagcaaga ccuucgacgc cgaguucaug gagaaccccg ccaaccagga gcugcucgag     1860

aacaucaucc agauccagac cguguucgag gacgugaaga ucgcggagcg ggagcugcag     1920

aagcuggccc uccccgacga gcagguccag cagcuggcca agacgcacua caccgggugg     1980

ggcaaccugu ccgacaagcu ccugagcacc ccgaucaucc aggagggguc ccagaaggug     2040

agcauccuga acaagcucca gaccacgucc aagaacuuca ugagcaucau caccgacaac     2100

aaguucggcg ugcagcagug gauccaggag cagaacaccg ccgagaccgc cgacuccauc     2160

caggaccgca ucgacgagcu gaccacggcg cccgccaaca agcggggcau caagcaggcc     2220

uucaacgucc uguucgacau ccagaaggcc augggggagg agcccaaccg cguguaccuc     2280

gaguucgcga aggagaccca gaacagcgug cggaccaacu cccgcuacaa ccggcugaag     2340

gaccuguaca agagcaagac ccucagcgac gacgucaagg cccugaagga ggagcuggag     2400

ucccagaagu ccagccucca gagcgagcgc aucggcgacc ggcuguaccu guacuuccuc     2460

cagcagggga aggacaugua cacgggccag cccaucaaca ucgacaagcu guccaccgac     2520

uacgacaucg accacaucau cccgcaggcc uacaccaagg acgacagcau cgacaaccgc     2580

gugcuggugu cccggcccga gaacgcccgc aagagcgacu ccgccaccua cacgaccgag     2640

guccagcaga gcgcgggcgg gcucuggaag ucccugaaga acgccggcuu caucagccag     2700

aagaaguacg accggcugac caagggcggg gacuacucca aggggcagaa gaccggcuuc     2760

aucgcccgcc agcucgugga gacgcggcag aucaucaaga acguggccag ccugaucgag     2820

uccgaguuca gccagaccaa ggccgucgcg auccgcuccg agaucaccgc cgacaugcgc     2880

cggcuggugg ccaucaagaa gcaccgcgag aucaacagcu uccaccacgc cuucgacgcc     2940

cuccugauca ccgcggccgg ccaguacaug caggcccggu accccgaccg cgacggggcc     3000

aacguguaca acgaguucga cuacuacacg aacaccuacc ugaaggagcu ccggcagagc     3060

uccagcucca gccagguccg ccggcugaag cccuucggcu ucgugguggg gaccauggcg     3120

aagggcaacg agaacugguc cgaggacgac acccaguacc ugcgccacgu caugaacuuc     3180

aagaacaucc ucacgacccg gcgcaacgac aaggacaacg gggcccugaa caaggagacc     3240

aucuacgccg uggacccgaa ggccaagcug aucggcacca acaagaagcg gcaggacgug     3300

agccucuacg gcggguacau cuaccccuac uccgccuaca ugacgcuggu ccgcgcgaac     3360

ggcaagaacc ugcucgugaa ggugaccauc agcgccgccg agaagaucaa guccgggcag     3420

aucgagcuga gcgaguacgu ccagcagcgg cccgagguga agaaguucga gaagauccug     3480

aucaacaagc ucgccaucgg ccagcuggug aacaacgacg ggaaccugau cuaccucacc     3540

uccuacgagu ucuaccacaa cgccaagcag cuguggcugc ccaccgagga ggcggaccuc     3600

aucagccagc ugaacaagga cuccagcgac gaggaccuga ucaagggcuu cgacauccuc     3660

acguccccgg ccauccugaa gcgcuucccc uucuacgagc uggaccucaa gaagcugguc     3720

aacauccggg acaaguucau cgccguggag aacaaguucg acauccugau ggugauccuc     3780

aaggcccugc agcuggacgc cgcgcagcag aagcccguca agaugaucga caagaagucc     3840

gccgacugga aggacuaccg ccagcggggc gggaucaagc ucagcgacac cagcgagauc     3900

aucuaccagu ccaccaccgg caucuucgag aagcgcguga agaucagcaa ccugcuguga     3960


<210>  1773
<211>  3963
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1773
augaccgagc agcagcgccc cuacaacauc ggccuggaca ucgggaccag cuccaucggc       60

ugggccguga cggaccagaa cgacaagcuc cugcacaucc gggggcacaa cggcaucggg      120

guccgccugu ucaaggaggg cgagagcgcc gcggagcggc gcggcuuccg gaccacccgc      180

cggcgccucu cccggcgccg guggcgccug cgguuccugg accgcaucuu cgacgccgag      240

cucgccaagg uggacccgaa cuuccuggcc cggcugaagc agagcaacau cagcccccuc      300

gaccccaaca agcagauguu cgggaacauc cuguucgacg acgcccaguu cgacgaccag      360

aaguuccacc agcaguaccc caccaucuac caccuccgca gcgcgcuggc ccagccggac      420

gcgccccagg ccgacgugcg gcugaucuac cuggcccucc accacaugau caaguuccgc      480

ggccacuucc ugaacggggc cgccgucgag aaguuccacg gcgggcagcu gcaccucgag      540

gccgaguucc aggagcugaa ccagaucuuc uccaaccagc agcgggacca cgugcagcug      600

cagacgagcu cccucgacga gguggccacc auccugaagg acaaccagcg cacccccagc      660

gaccggcagc gcgagcuguc cgcgcgccuc uucguccccg uggacaagac caucgacaag      720

gagaacaaga agcuggccac ggagcugcuc aaggccgugc ugggccugaa ggccaaguuc      780

gacgucaucc ucggcgugga ggccaccgac ccgcaggcgu ucaagcugca guucagcucc      840

gacgacuucg acgacaagau cgccgagcug gccagcgagc ucaccgacga ggccaacgag      900

auccugaccg ugcugcagcg gcucuacuuc ucccugaacc uggccgacau ccucacgaac      960

cgccagacca agcaggucgc ggacagcauc uccaccgcca ugaucaaccg guacgacgac     1020

cacaagcacc accugaagau gcugaaggag cucgagaccc aggugacgca ggagcagcgc     1080

gacgggcuga agaaggccua cgacgaguac aucgacggcg acaagcccac caucgacacc     1140

uucuacacca acgugaagaa gaaccuggac gacagcgagc ucgccaacca gaucaagucc     1200

cugaucgacc ugcaggccuu caugcccaag cagcggagca aggagaacgg gagcaucccc     1260

caccagcucc accagcgcga gcuggaccag cugaucgagg uccagggcaa guacuacccg     1320

uggcugcucg cggccaagga caagcuggac gcccugaucg ccuuccgggu gcccuacuac     1380

gugggcccca ugaucgaccc caacgagucc gacgacccgc agaacacgaa guucgcgugg     1440

augguccgca aggaggccgg ggagaucacc cccuggaacu ucgaggagaa gguggacaag     1500

accggcagcg ccaccaacuu caucaagcgg augacgacca ccgacaccua ccuccugggg     1560

gagcccgugc ugcccaagaa cucccuccug uaccagcagu ucacgguccu gaacgagcuc     1620

aacaagcuga agguggacgg caaggcccug accguggacc agaagaagua ccucuacgag     1680

cacgucugca agaagcagcg ccgggugacc cugaagaagc ugagcgacug ccucauggcc     1740

cgcggggacu ucccgauggg cgcgaucuuc accggcacga ccgaccagaa gaacaugaac     1800

aacacccuga agaccuacca ggacuuccag gagaucuucg gggacgagau cgacaacccc     1860

gaccggcagg ucgacuucga gaagaugauc gaguggucca cgaucuucga ggaccgcgag     1920

aucuucaagc ugaagcucgc cgagcugaac uggcugacca gcgagcagcu cgagcgggug     1980

uccaagaagc gcuaccaggg cugggggcgg cugagccaga agcugcucac ccacaucgug     2040

aacgagaacg gcgagcgcau caucgaccag cuguggaaca ccaaccagaa cuucauggag     2100

aucauggggc aggacggcgu caagcaggcc auccaggagc acaacggcgc caacguggcc     2160

cagaccggga ucaacgagac gaucgacgag cuguacacca gcccccagaa caagaaggcg     2220

auccggcaga uccuccuggu ggucgacgac aucaagaagg ccgugggcua cgcccccgcc     2280

aacaucauga uggaguucgc ccgcgaggac cgcgacgacc accggcugau cgcgucccgc     2340

gcccggcagc ucgagaagac cuaccaggag aucuccaaga acugguucga caacgagagc     2400

gugacccagg agcugaagga ccagaucaag aacaagacga aguucaccga ccgccuguac     2460

cucuacuuca uccagggcgg gaucgaccug uacaccgggc acccgcugaa caucgaccag     2520

cucuccagcu acgacaucga ccacauccug ccccaguccu uccugcucga caacagccug     2580

aacaaccggg uccugaccuc ccagagcgag aaccgcgugc acaaggagga ccggcucccc     2640

ggcgagacgg uggccaacaa gaugcgcccc accuggaaga ugaugcggga gcagggccug     2700

augacccagc gcaaguacaa caaccugacc cucaagccgg accagaucuc caaguucaac     2760

aaccgggggc aguucaucaa ccgccagcug gucgagacgc gccaggugau caagcuggcc     2820

gcggacauca ugaaccagaa guaccaggcc gaccagacca agguggucac cgugaaggcc     2880

aaccucaccc accacaugcg ggagaaguuc aacuucuaca agagccggaa cgugaacgac     2940

uaccaccacg ccuucgacgc cuaccugacg gucuucgugg gcaacuggcu gcucaaggag     3000

uaccccaagc ugcagcccua cuucguguac ggggacuucg cgaagaccgg caccaacaac     3060

cugaaguccu ucaacuuccu cuaccgcuuc gagcgggacc acaagcugaa cgacaagggc     3120

gccaucgaca ccgacaagga ccagcagcug ggguacauga agcgcaucua ccgguucaag     3180

aagaugcucg ucacgaagca gcuggagacc aacgagggcg cccuguacaa gcagaccauc     3240

uacaaggccc cggccaacga gaagaacacc aagaagaacc gcucccucau caacacgaag     3300

aagaaccggc ccagcgacau cuacgggggc uacucccaga gcgugaccgc guacaugucc     3360

cugguguucu ucaagaacac cuucaagcug aucaagaugc cccucgucgc cgccaacgcc     3420

aucaagaaca gccuguccag cuaccacgac accauccuga agauccucgg guccaacgag     3480

uucaaggugc ugauccccaa ggugaagaug aaccagcuga ucaaggaggg cgacagccuc     3540

uacacggucg ugggcgugua caagcacaac gcccagcagc ugauccuguc cgacaacagc     3600

auggggauca ucaccaagcc gauccgcgag aucugcgacu ccgaccucga cagcgucuuc     3660

aacgagcugg uggacaacau cgacaaguac cugaaccucu acaagaucga ggacaagcug     3720

accaucaagg agaaguucua cgcgcugaag aacaaggagu ccaaguucaa gugcaucagc     3780

gacauccucg ugggcaccca cugcaacgcc gggcgcucca accugaagau caucggccug     3840

agcagcuacu gggggauguu cuccggcaac aacgacggcc ucaagaucaa cgagaacagc     3900

cagcugaucu accagucccc cacggggcug uucgagcacc gggucaaggu gagcgaccuc     3960

uga                                                                   3963


<210>  1774
<211>  3972
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1774
augaaggacu ucuacaucgg gcuggacaug gggaccgaca gcgugggcug ggccgucgcc       60

gacgaggacu acaaccucug caaguucaag gggaaggcga uguggggcgu gcgccuguuc      120

gacgaguccg ccaccgccgc cgagcggcgc ggguuccgga cggcccgccg gcgcgcgcag      180

cggaagcgcc agcggauccu guggcuccag augcuguuca acgaggagau cagcaagaag      240

gacaucgccu ucuuccagcg ccugaacgag uccaagcucu accuggagga caagagcacc      300

aacgugcccu acgccgucuu cgccgacgag uccuucaccg acguggacua ccacaagcug      360

uacccgacca ucuaccaccu ccggaaggag cuggugcaga gcgacgagcc ccacgacguc      420

cgccuggugu accucgcgcu gcaccaccug aucaagaacc ggggccacuu ccucuucgag      480

aacgugggcu ccgacuucga gaaggagagc uccuucgagg uccuguucaa gaaccugcag      540

aacuacaucc ucggggagua cgagcuggcc cuggagugca ucagcccccu ccagaucgcc      600

gacauccuga aggacaaguc caagcgcaag acgaccaaga gcuccgagau cugcaagcug      660

cucggcauca gcaagaaggg gcagccccgg cugaacgcca ucgugcugcu caugugcggc      720

caguccguga agcuggccga ccuguacggg gacgagaacu acaacaagga ggagaagucc      780

agcaucagcu ucgagaaggg cuacgaggac aacgaggaga ccuaccgcga gcuccugcug      840

gagcgguucg agcucaucga gaagaucaag gcgcuguacg acugggccau ccuggccgac      900

auccucaagg gcgagaccua caucuccuuc gccaagguca agacguacga cgagcaccgc      960

cacgaccugc agcagcugaa ggccuucgug aaggagcggu gccgcagcga cuacaacgag     1020

aucuuccggg agaccagcga caagcucaac aacuacaccg cguacugcgg gaaguucaag     1080

gagaacggcc gcaacggggu gauccugcac uccgccaacc agaccgacuu cugcaaguac     1140

cucaagagca aguucgacaa gcucgacaag uccggcuacg aggacauguu cgagaagauc     1200

gagaacggca gcuucaugcc gaagaucguc gugaaggaca acggggugau ccccaugcag     1260

gucaacgagu ccgaggugcg cgccauccug aagaacgcca gcggcuaccu ggacuuccuc     1320

aacaaccagg acaaggacgg gaucacggug gccgacaaga uccugcagau cuucgaguuc     1380

cggauccccu acuacgucgg cccccugaac gugcacuccg acaacagcug gcucgugcgc     1440

aaggacggga agaucuaccc guggaacuuc aacaagaucg ucgacgugga caaguccgcg     1500

gaggaguuca ucaacaagcu gaccgccaag ugcaccuacc ugcccgaccg ggacgucauc     1560

cccaagaaca gcauccugua cucccgcuac auggugcuca acgagcugaa caaccugaag     1620

cucgacggca agaagcccga cgugagcuuc aagcaggccg ucuucaacga ccuguucaug     1680

caccggcgca aggugaccca gaaggcccug cgggaguaca ucaaggccca gacgggcacc     1740

gagccggaga ucaccgggau cgacggcgac uucaaggcga acaugcgccc cgccaucgag     1800

cucgccgggu acaaccuguc cgagcccgac aaggaggaga ucaucaaggc caucaccauc     1860

uucggcgacg acaagaagcu gcucaagcgg cgcaucaagg acaaguacag cgagaugcug     1920

uccgaggacg agaucaagaa gaucugcaag cugaaguaca aggacugggg gcggcucagc     1980

cgcgaguucc ugacggagau cuucgacgug gacaccgcga ccggcgagug caaggacaac     2040

aucaucaaca cccuguggaa cacgaacgac aaccucaugg agcugcuggg cgccaaguac     2100

aacuucaccc aguccaucga gcaggccauc agcaagucca cccacaaggc cagcaucgag     2160

aagauggucg aggacaugua cgugagcccc aaggugaagc ggaccgucuu ccagucccuc     2220

aagaucauga aggagaucgu gaagaucaag gggcacgccc cgaagaaggu guucgucgag     2280

augacgcgcc acgacggcac caagggggac aacggccgga ccaagagccg caagaagcag     2340

cuggaggagc uguacaagaa gugcaaggag gacuccaccg agcucuggga cgagcuggag     2400

aagacggacg acgacgaguu ccggcaggac aagcuguucc ucuacuacac ccagaugggg     2460

cgcugcaugu acagcggcga gcggaucgag cugcgcgacc uguucaacaa gaaccucuac     2520

gacaucgacc acaucuuccc ccgcuccaag gugaaggacg acagcaucga caaccgggug     2580

cuggucaaga agcagaucaa cucccacaag gacaacgacu acccccugga cagcaccgug     2640

cgcgagaaga ugcggggcca cuggcaguac cuccugcgcc acggguucau cuccaagaag     2700

aaguacgacc ggcugacccg cgugacgccc cucaccgacg acgagcuggc ggacuucauc     2760

gcccggcagc uggucgagac cagccagucc accaaggccg uggccacccu ccugaagacg     2820

cuguacccga ccaccgagau cguguacguc aaggccgagc ucgugagccg cuuccggacc     2880

gaguacaagu ucaucaagug ccgcgaggug aacgaccugc accacgcgaa ggacgccuac     2940

cugaacaucg ucgugggcaa cguguacaac guccgguaca cgcacuccaa ggccaucuuc     3000

aucaaggggc uccagaccaa gaaguacagc cugaaccgca uguucgccuu cgacaccccc     3060

ggcgccugga acaucgagaa cgagaagucc cugagcaucg ugaagaagac cgugaugaag     3120

aacaacaucc ucuacacgcg guacuccuac augaagaagg gcgggcuguu caagauccag     3180

ccccugaaga agggcaacgg gcaggcgccc cucaagaagg accugccgcu gagcgacauc     3240

ggcaaguacg ggggcuacga caagcccacc gccagcuacu ucuccuacau caaguacgag     3300

ggcaagaagg ggaaggagug ccgccagcuc gucgccaucg acaacuaccu gaagaaguac     3360

uacgaggaga accccaucga guaccugacc aaccggcucg gccugaagaa ccccgccgug     3420

cugaucccgg ugaucaagua caacagcugc aucgagaucg acggguuccg caugaacauc     3480

uccagcaagu ccaacgaggg caagaccauc gucuacaagc ccgcggugca gcucgugcug     3540

ggggccgaga aggaggccua cgucaagaag aucagcaagg ugcuguccaa gcccgaggag     3600

cagaagauca cgagcuacga cggcgugucc gccgaggaga acaucgagcu guucgacgcc     3660

cugaccgcga agaugaccaa caccaucuuc aagaucaagc uggaggacau cggcaacaag     3720

aucaagagcg ggcgggacaa guucgucacg cucgacguga agaagcagug cuacgugauc     3780

aacgagaucc ugaagaucau gcacugcaac gucgugaccg gcgaccuguc ccucaucggg     3840

gccgccaaga aggccggcau cgugagcacc aacuccgccc ugagcgagau caagaagguc     3900

uccagcauca ugcugaucaa ccaguccgug accggccucu ucgagacgaa gaucgaccug     3960

cugaacaucu ga                                                         3972


<210>  1775
<211>  3975
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1775
augcgcgcca agggcaagac ccugaucggg accagccucu uccgggaggg caacacggcc       60

gcggagcgcc ggggguaccg caccacccag cggcgccuga agcggcgcaa gcggcgccug      120

aagcuccugg aggccuucuu cgaccccuac aucuucgagg uggacgagaa cuuccuggcc      180

cggcuccacg aggccgacau cuccccgaag gacgagaaca agcgcuucug gggcagccug      240

cuguucccca acgagaagga cgccaacuuc uaccggaagu accccaccau cuaccaccuc      300

cgcaaggcgc ugauggagga ggaccggaag uucgacgucc gcgccaucuu ccuggccauc      360

caccacauca ugaaguaccg ggggaacuuc cucagccccc ugagcgugga cuccuucaag      420

caggagggcc uggagcucaa ggagcagcug gaccgccagc ugaagcgggu ggccgagcuc      480

uacaacgagc ugaacacgga cgucaagauc aacgugagcg acguggagac cauccaggac      540

aucaucaucg acaagaacaa gucccgccgg gacaaggcgg gcgcccugaa gaagcuccug      600

gccgucagcu ccgaccgcaa ggagaucaac aagcugaacg ccagccucgc caaggagcug      660

uccaacgcga uccuggggau gaaggcccag uucgccaacc uccugcuggc cgacgaccuc      720

gagaucgaca agaacaagaa gaacuucagc cugcuggaca ccaacgccga ggagaacgug      780

uccggccucc uggagcuggu gaacgagaag caggcggagc uguucgacgc caucaagacc      840

auccacgacc agaucauccu gaacgagauc gucccggacg gcaagagcau cucccagagc      900

augguggaca aguacgaggc ccaccgcgag cagcuccagg ugcucaaggc ccuguacgag      960

aagauggacg ccaagcagau caaggcgauc aagaaggccu acucccugua caucgacggg     1020

aaggacccca aggccaagaa ggucgacaag cgggcccacc agaaccgcgc cgacuucuac     1080

aaggagguga agaaggugcu cgucgagcug gacgacagcc gggcggagaa gcugcucggg     1140

cugaucgaga ugggccaguu ccugcccaag cagcgcacgg ccaugaacgg cgugaucccc     1200

aaccagcucc accaggugga gcuggacaag aucaucgaga ugcaguccaa guacuacccg     1260

uggcuggccg agaagaaccc cgucaagagc caccagaagc gggcccccua caagcuggac     1320

gagcuccuga ccuuccgcgu gcccuacuac guggggccga uggucaccag caagaagcag     1380

cagcagaccu ccaacagcaa cuucgcgugg auggugcgga agcaggaggg cgagaucacg     1440

cccuggaacu ucgaggagaa gguggacacc aucgccuccg ccaacgacuu caucaagcgc     1500

augaccgcca aggacaccua ccuggucggg gaggacgugc uccccgccaa cagccugcug     1560

uaccagcggu acaagguccu caacgagcug aacaacauca aggugaacgg ccgccggcug     1620

cccguggagc ucaagcgcga ggcguacguc gcccuguucg agaacggcga cuccaagacg     1680

gugagcgcca agugcuucaa gaacuggcug aacgugaagc ucaagaaccu gaacucccug     1740

aagcucgagg ggcugagcac cggcaccaag uucgacaaca accuguccac cuaccacgac     1800

cucaagaaga ucuucgggaa ggccgucgac gacgccggcc ggcagaagga cuucgagcag     1860

aucaucgagu ggcugacgau cuucgaggac aagaagaucc ugcgcaucaa gcucaaggag     1920

aucgaguggc ugaccgagga gaacaucacc cagcugaccc ggcucaacua caacgggugg     1980

ggccgccuga gccggcgccu gcucacgggc aucugcgacu ccaacgggca gcggaucaug     2040

gacuggcugu gggacaccac caacaacuuc augcagaucc agagccagcc ggacuuccag     2100

aaggcgaucg uggaguacaa cuccgacaag cuggagcagg gcgacaucga gcagauccuc     2160

ggggaggccu acaccagccc ccagaacaag aaggccaucc gcaaggugau caaggucgug     2220

caggacgugg agaaggccau gggcgccaag cccacgucca ucagcaucga guucacccgc     2280

uccgacgggc ggagcgagcu gaccaacucc cgcaugaccc agcugcagaa gacgcucaug     2340

agccugagcg aggagaugac cgaccaggcg cugcucgccg aguucaagga cuuccaggcc     2400

uccaagaaga agcugaccaa caaguacuac cuguacuuca cccagcucgg ccgggacaug     2460

uacacgggcg agccccugaa caucgaccag cugagccucu acuccgacgg ggaccacauc     2520

cugccgcgca gcuucaucaa ggacgacucc cuggacaacc ggguccucac caagcgcgcc     2580

gugaacaacg agaaguccga caccgugccc gucugcgagu acggcgcgaa gaugaaggac     2640

uucuggaacg accuguacga caacgggcug aucuccaagc agaagcucgc caaccugcug     2700

accgaccccg acgagaucgg ccccuacaag gccaacgggu ucauccggcg ccagcucgug     2760

gagaccagcc aggugaucaa gcuguccgcc gagauccugc agacgcucua ccaggacacc     2820

aagaucaucg agguccggca gaaguacacc cacgagcugc gcgagcacuu caacuucgug     2880

aagauccggc agcugaacga cuaccaccac gccuucgacg cguaccucac cgccuucagc     2940

ggccgcuacc uguaccagcg guacccgaag cugcgcggcu acuucgugua cggggacuac     3000

aacaaguucg acgccaagaa ggaccggaac uaccucaagu ccuacaacuu ccuguacgac     3060

cuggaggagg gcaagacgga ccagaucguc gacaacgugg agcacaaggu guucaugaac     3120

cgccaggacg ccaucaacca gcucaagaag gccuacgagu acaaguacau gcuggucagc     3180

caggagaccu acaccaacuc cggggcgaug uucaaggaga ccaucuaccc cgugaacccc     3240

aagcagaagc ugauccagaa gaagaacgac aagcccgugg acaucuacgg cggcuacagc     3300

aacaacuacg acgccuaccu cuccaucguc cggcuggagg acaagaaggg gaucaaguuc     3360

cgcguggugg gcaucccgau gcggaacaag gccgagcugg acaaccucgc caaggaggac     3420

cacaacgggu acaugcagcg ccugaacgag aucgucgacg agcagaucaa cagcuccggc     3480

aagaagaaga cguuccggau cauccugccc aaggugcacu ucaagcagcu cguggaggac     3540

gacagcggga aguucaugcu gggcagcgcc gcguaccccu acaacgccaa gcagcugguc     3600

cucuccguca agagcaucga gucccuggcc aacaaggacg ccagcgaccg cgagcuggug     3660

gcgguguacg aggagauccu caacguggug aacgacaagu ucacccugua cgacgcccgc     3720

caguuccgga acaagcugac cuccagcaag gacaaguucg ucgagcucaa caccgccgac     3780

aaggugacgg agcugcugga cauccucgac ggccugcacg ccaacccggu ggucaagaag     3840

cugaagauca ucuccgggag ccccuucggc uuccuccagc gccccuccgg gaucgugcug     3900

accgagaacg ccaagcugau cuaccagagc cccaccggca ucuucgagcg gguggucuac     3960

cuccgcgacc uguga                                                      3975


<210>  1776
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1776
auggagggcc agaugaagaa caacgggaac aaccugcagc agggcaacua cuaccucggg       60

cuggacgugg gcaccagcuc cgucgggugg gccgugaccg acacggacua caacgugcug      120

aaguuccgcg gcaagagcau guggggcgcc cgccucuucg acgaggcguc caccgccgag      180

gagcggcgga cccaccgcgg gaaccggcgc cggcuggccc gccggaagua ccgccugcuc      240

cugcuggagc agcuguucga gaaggagauc cggaagaucg acgacaacuu cuucguccgc      300

cuccacgaga gcaaccugug ggccgacgac aaguccaagc ccagcaaguu ccuccuguuc      360

aacgacacca acuucacgga caaggacuac cugaagaagu acccgaccau cuaccaccuc      420

cgguccgacc ugauccacaa cagcaccgag cacgacaucc gccugguguu ccucgcccug      480

caccaccuga ucaaguaccg gggccacuuc aucuacgaca acuccgcgaa cggggacgug      540

aagacccucg acgaggccgu cagcgacuuc gaggaguacc ugaacgagaa cgacaucgag      600

uucaacaucg agaacaagaa ggaguucauc aacgugcugu ccgacaagca ccucacgaag      660

aaggagaaga agaucagccu gaagaagcug uacggcgaca ucaccgacuc cgagaacauc      720

aacaucagcg ugcucaucga gaugcugucc gggagcucca ucagccuguc caaccucuuc      780

aaggacaucg aguucgacgg caagcagaac cugagccugg acagcgacau cgaggagacc      840

cucaacgacg ucguggacau ccugggcgac aacaucgacc ugcucaucca cgccaaggag      900

guguacgaca ucgccguccu gaccuccagc cuggggaagc acaaguaccu cugcgacgcg      960

aagguggagc uguucgagaa gaacaagaag gaccugauga uccucaagaa guacaucaag     1020

aagaaccacc ccgaggacua caagaagauc uucuccagcc ccacggagaa gaagaacuac     1080

gccgccuacu cccagaccaa cagcaagaac gugugcuccc aggaggaguu cugccuguuc     1140

aucaagcccu acauccgcga cauggucaag agcgagaacg aggacgaggu gcggaucgcc     1200

aaggaggugg aggacaaguc cuuccugacc aagcucaagg gcaccaacaa cagcgucgug     1260

ccguaccaga uccacgagcg cgagcugaac cagauccuga agaacaucgu ggccuaccuc     1320

cccuucauga acgacgagca ggaggacauc uccgucgugg acaagaucaa gcugaucuuc     1380

aaguucaaga uccccuacua cguggggccc cugaacacca agagcacgcg guccuggguc     1440

uaccgcagcg acgagaagau cuacccgugg aacuucucca acgugaucga ccuggacaag     1500

accgcgcacg aguucaugaa ccggcucauc ggccgcugca ccuacacgaa cgaccccguc     1560

cugcccaugg acagccugcu cuacuccaag uacaacgugc ugaacgagau caaccccauc     1620

aaggugaacg gcaaggccau cccggucgag gugaagcagg ccaucuacac cgaccuguuc     1680

gagaacagca agaagaaggu gacccgcaag uccaucuaca ucuaccuccu gaagaacggg     1740

uacaucgaga aggaggacau cgucagcggc aucgacaucg agaucaagag caagcugaag     1800

ucccaccacg acuucaccca gaucgugcag gagaacaagu gcacgcccga ggagaucgag     1860

cggaucauca aggggauccu cguguacagc gacgacaagu ccaugcugcg ccgguggcug     1920

aagaacaaca ucaagggccu cagcgagaac gacgucaagu accuggccaa gcugaacuac     1980

aaggaguggg ggcgccucuc caagacccug cugaccgaca ucuacaccau caaccccgag     2040

gacggcgagg ccugcagcau ccucgacauc auguggaaca cgaacgcgac ccugauggag     2100

auccugucca acgagaagua ccaguucaag cagaacaucg agaacuacaa ggccgagaac     2160

uacgacgaga agcagaaccu ccacgaggag cuggacgaca uguacaucag ccccgccgcc     2220

cggcgcucca ucuggcaggc ccugcggauc guggacgaga ucguggacau caagaagucc     2280

gcgccgaaga agaucuucau cgagauggcc cgcgagaaga aguccgccau gaagaagaag     2340

cggaccgaga gccgcaagga cacccuccug gagcuguaca agagcugcaa gagccaggcc     2400

gacggcuucu acgacgagga gcuguucgag aagcucucca acgagagcaa cucccggcug     2460

cgccgggacc agcucuaccu guacuacacg cagauggggc gcagcaugua caccggcaag     2520

cggaucgacu ucgacaagcu gaucaacgac aagaacaccu acgacaucga ccacaucuac     2580

ccccgcagca agaucaagga cgacuccauc accaaccggg uccucgugga gaaggacauc     2640

aacggggaga agacggacau cuaccccauc agcgaggaca uccgccagaa gaugcagccc     2700

uucuggaaga uccugaagga gaagggccug aucaacgagg agaaguacaa gcggcucacc     2760

cgcaacuacg agcugaccga cgaggagcug uccagcuucg uggcccgcca gcucgucgag     2820

acccagcagu ccaccaaggc gcuggccacg cugcucaaga aggaguaccc gagcgccaag     2880

aucguguacu ccaaggccgg gaacgugagc gaguuccgga accgcaagga caaggagcug     2940

cccaaguucc gggagaucaa cgaccugcac cacgcgaagg acgccuaccu caacaucguc     3000

gugggcaacg uguacgacac caaguucacc gagaaguucu ucaacaacau ccgcaacgag     3060

aacuacuccc ugaagcgggu cuucgacuuc agcgugcccg gcgccuggga cgccaagggg     3120

uccaccuuca acacgaucaa gaaguacaug gccaagaaca accccaucau cgcguucgcc     3180

ccguacgagg ugaagggcga gcuguucgac cagcagaucg uccccaaggg gaagggccag     3240

uuccccauca agcaggggaa ggacaucgag aaguacggcg gcuacaacaa gcucagcucc     3300

gccuuccugu ucgccgugga guacaagggg aagaaggccc gcgagcggag ccuggagacc     3360

guguacauca aggacgucga gcucuaccug caggacccca ucaaguacug cgaguccgug     3420

cugggccuca aggagccgca gaucaucaag cccaagaucc ugauggggag ccuguucucc     3480

aucaacaaca agaagcucgu ggucaccggc cgcagcggca agcaguacgu gugccaccac     3540

aucuaccagc uguccaucaa cgacgaggac agccaguacc ugaagaacau cgcgaaguac     3600

cuccaggagg agcccgacgg gaacaucgag cggcagaaca uccugaacau caccagcgug     3660

aacaacauca agcuguucga cguccucugc acgaaguuca acuccaacac cuacgagauc     3720

auccugaaca gccugaagaa cgacgugaac gagggccgcg agaaguucuc cgagcucgac     3780

auccuggagc agugcaacau ccugcuccag cugcugaagg ccuucaagug caaccgggag     3840

agcuccaacc ucgagaagcu gaacaacaag aagcaggccg gggugaucgu caucccgcac     3900

cuguucacca agugcagcgu guucaaggug auccaccagu ccaucaccgg ccucuucgag     3960

aaggagaugg accugcugaa guga                                            3984


<210>  1777
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1777
augaagaacu acagcaucgg ccuggacauc gggaccggcu ccguggggug ggucugcauc       60

gacgagaacu accagauccu ccgcuacaac aaccgggccg ccuucggcgu gcacgaguuc      120

gagaccgcgc acguggccga ggagcgccgg aucaagcgcg gggcccggcg ccgguacaac      180

cgccggaaga agcgcaucca gcugcugcag agccucuucg ucccccacau gcagcagaag      240

gccuucuucg ccccgacgau caccgagcac uacuggaaga acgacaacca guucgagaac      300

cggacccugu ccgaggugcu gaugagccuc ggcaugaacc ccaacaccua ccccacgauc      360

uuccaccucc gccacgagcu gaucgagucc agcgugaaga aggacgcgcg gcugaucuac      420

cuggcccugc acaaccucgu caaguuccgc ggccacuucc ugcaggaggg guccaccugg      480

aacgccaaca ugagcgacgc caaccugucc gagcaguucg agcagcuccu gcacaucuac      540

accgagaugg aggagcugcc ccaggugagc cucaccgcgc agcaguccac ggacguggag      600

cacauccugc ugaacaacca caucagcaag gccgagcggg cccgccagcu caccgcccug      660

uucgagcgga ccuacgagcc guucuucaag cugaucgucg gccucgacau cgccgcggcc      720

cagcuguuca agcaguccga gcagcagcug cucuacaagg agagcaagac caaggugaac      780

cugacggccg agacccugcc cgaggugcuc cagaccaugc uggagcccga ggucgccuuc      840

aucgaggccg ugcagcccuu cuaccagcag accgugcugu ucgaccuccu gaaggggcag      900

uccuacgucg cggccgccaa gguggaggcc uucaaccagu acgagcgcga ccuggcccuc      960

cucaagagcg ugaucaacga ggucggcucc gagaagcagu accgggcguu cuucaucacg     1020

agcaagcaga agaagcgcca guucgagcag aagccgaaca uggagcugcu gugccaccug     1080

gaccgguuca accgcgagaa gaaguacgag gagaccuucu acaaggaggu guccaagcug     1140

cucaccgccc ugaucaagga ggccaccggg gaggugaagg agcacaucga gcagaucauc     1200

caggccaugg agagccagag cuuccugguc aagcagaagg gcauccagaa cgccuccauc     1260

cccuaccaga acagccucua cgaggcggag augauccugc gcaaccagca ggccuacuac     1320

cccuggauca cggacgaguu caucgagaag gugaagcagc ugaucgccuu ccggaucccc     1380

uacuacaucg gcccgcucgu gcagggggac gagcagcccu ucggcuggcu gcagcgccag     1440

gccgacggga acaucacccc cuggaacuuc gacgagaagg ucaacaaggc ggccuccgcc     1500

gagagcuuca ucacccggau gaccaagaac ugcacguacc ugaaggagga gaaggugcug     1560

cccaagcagu cccucaugua cgagaaguuc acccugcuga acgagcucaa cagcguccag     1620

auccgcucca gccuggagca gccgcacaag aagcaccggc ugugcgagga caugaagcag     1680

uggaucaucg agcacguguu caagaagacc aagaccguga cgcacaagac ccucauccag     1740

gcccugaaga agucccccua caaggacgag cuguacgacg agagcaccgg caacuggaag     1800

gacgucuacg gcacccagaa ggaggaccgc uucgccucca gccucuccac guacaucagc     1860

cugaaggaga ucguggggga caucguggac aacgcguuca uggaggagau caucuacugg     1920

cugaccgucu ucaccgaccg ggacaucauc acccucaaga ugaacgacaa guaccccuuc     1980

aucacggagc agcagaucca gcgccugcug ucccucaaca ucagcggcug ggggcagcug     2040

ucccagcggc ugcuccagag cauccccgug gacgacauga accgcuccgu gaucgaguac     2100

auggagaagc acgccgucaa cuucauggag cugcugagcc cgcggcagag cgaccucgcc     2160

uacaagaucg agcaggucaa caagcagcac uccaagaaga uccacaagau ccgcuacgag     2220

gacaucgagc agcugaccgg cagccccgcc cugaagcggg ggaucuggcg cuccgugaag     2280

aucaucgagg agcucaccgc caucuucggc gagcccaaga acaucaugau cgagguggcg     2340

cgggaggacc aggccagcca ccgcaccaag ucccggaagg agcaguggga gagcaucggc     2400

aagucccugg ccaaggagga gaaggagcug aaggccuucu ucaaggagau ggcccaguac     2460

cccaagcagc aguucgaccg cacccgguuc uggcucuacg ugacgcagca ggggaagugc     2520

cuguacaccg gcaagacccu gcgccucgag gaccugagcu ccuacgagau cgaccacauc     2580

cugccgcgca acuucgugaa ggacgacagc aucgacaacc ucgcgcuggu cuaccccaug     2640

gccaaccagg agaagaacaa ggccgggaac aacaagaugc cccuggagau caucgacgcc     2700

aagcagcagc ccgcgaugau ggcccacugg aagcggcuca accagcugaa gcugaucucc     2760

gacagcaagc uccacaagcu gaugaagccg uccuucaacg acguggacaa ggagaacuuc     2820

aucgcccgcc agcuggugga gacccggcag aucaucaagc acguccgcga ccuccugcag     2880

gagcgguucg agcacacgga cauccacgug gugaaggcca agauggucag ccaguuccgc     2940

aaguucuccc ggauccccaa gauccgcgac uacaacaaca agcaccacgc cauggacgcg     3000

cuguucacca ccgugcucau ccaguucauc cugcagaagu acggcaagaa cuuccugcag     3060

uucgaccucc agcacaagga caucagcaag aaguggucca agaucgccca gagcaccaag     3120

cacuucuucc uguuccagca guucgccgag cagcagauca agagccccgu gacgaaggag     3180

cuggucuccg gggugcagua cuucgagcac aucuucuacg agcagagcug gcagaccacc     3240

aagaugaccc agacgaucga cuccaacuuc uacaaggaga ccaucuucag cccgaagcuc     3300

aagaccgcca aguacaccuc cgccaaggcg gagaaguucg ugcacgacgc caucgucaag     3360

uacgccgugu gcggcaucca guacacgagc cugaagaaga acaaggagca gcaccacgug     3420

cugcucaccg acaucaacgu caucgagcac uaccagcuga aggaggccuc ccugaacgag     3480

cucgcccugc accuggcgaa gcgggagagc aaggacgacg ugaucgacgc ccagaugcuc     3540

guggucaucc ccaaguacaa gaagaucaug cugaacggcu cccccuacua cugcaucagc     3600

uccgacgagc gccacaucgc cacccaguuc gugcugcgga aggaccuccu ggaggccgug     3660

cugcacguca acgacgagac cagcguggac gagcuccgca cgcuguuccg guccaucgug     3720

gaggagauga ugacccagua caaggucuac aacaaggaca ccauccgcaa caagaucgac     3780

gcguucaucg agaccgagcu gaucgacgug gagacguucc aggugggguu caccgagauc     3840

aagaagaugg ucgccgccaa cgcccagcgg agcgacaagu ucggcgggcg ccuccgccag     3900

gugaugaagg uggaggacau cuacaucauc gacgagucca ucaccggccu gcgguaccgg     3960

aagccccgca agcuggucaa guga                                            3984


<210>  1778
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1778
augaagaaga acuacaccau cggccuggac aucgggaccg ccagcguggg cugggccguc       60

cucacggagg acuacgagcu gaucaagcgc aagaugaaga ucuccgggaa cacccagaag      120

aaggcgguga agaagaacuu cuggggcgug cggcuguucg agcaggggga gaccgccgag      180

ggccgccggc ucaagcgcac cacgcggcgc cggaucgccc gccggcgcca gcggauccag      240

uaccugcgca ccaucuucga cgagggcaug aaccaggucg acgccaacuu cuucgcccgg      300

cuggacgaga gcuucuccau caucgacgag aaggagaacg agcgccaccc caucuucggg      360

aacguggcgg aggaggccgc cuaccacgag caguucccga ccaucuacca ccuccgggag      420

caccuggcca acagcuccga gcaggcggac cugcgccucg uguaccuggc ccuggcccac      480

aucaucaagu uccggggcca cuuccucauc gagggggagc ugaacaccga gaacagcucc      540

gucagcggca cguucgagca guucaucaag guguacaacg agaccuucaa cguggagaag      600

gcccuggacc ucaccgucga ccuggacggg aucgccgagc agaagaucuc ccgcaugaag      660

cgggcggagc ugauccucag ccuguucccc gaggagaagu ccaccggcga cuucgcccag      720

uucaucaaga ugaucguggg caaccagggg aacgugaaga agacguucag ccugaacgag      780

gacgccaaga uccaguucuc caaggaggag uacgaggaga accucgagac ccugcuggcc      840

gagaucggcg aggacuuccg cagcgucuuc gacgccgcga aguccgugua cgacgccauc      900

agccucgcca acauccugaa ggugaccgac gccaccacgc gggccaagcu guccagcucc      960

auggucgugc gcuucaccga ccacaaggag gaccucaaga cccugaagcg cuucgugcgg     1020

gagaaccugc ccgacgagua cgacgaccuc uucaagaaca agaaggucgc gggguacgcc     1080

ggcuacaucg acggcaacga gacccaggag gccuucuaca aguaccugaa gaagacgcug     1140

gccaaggcca ccggggcgga gggcuuccuc gccaagaugg agcaggagga cuuccugcgc     1200

aagcagcgga ccuucgacaa cggggugauc cccuaccagc ugcaccucga ggagcugaag     1260

gccaucauca agaaccagaa guccuacuac agcuuccugg acgaggagaa gaucagccag     1320

cucaugaccu uccgcauccc guacuacgug ggcccccugg ccaaggagaa ggggcaguuc     1380

gcguggcuga cgcggaagga gaugggcaag aucacccccu ggaaccucaa cgagaagguc     1440

gacaucgaga aguccgccac cgacuucgug gagcgcauga ccaacaacga cagcuaccug     1500

cccauggaga aggugcugcc gaagcacagc cuccuguacg agacguucac cgucuacaac     1560

gagcugacca aggugcggua caucgacgac aacggccgcg cccagaacuu cuccagcaag     1620

gagaagcggc agaucaucaa cgagcuguuc aagcagcagc gcaaggucaa gaaggagaug     1680

cuggaggccu uccugaagaa cgaguacggg aucgagaacc ccaaggugga gggcaucgag     1740

aaguccuuca acgccugccu ggggaccuac cacgaccuca ucaagcuggg cauccggccc     1800

gagcuguucg agcagcccga gcacgagcag caguucgagc agaucgugaa gauccucacg     1860

gucuucgagg accgcaagau gcggcgcaag cagcuggaga aguucagcaa cauccugacc     1920

gaggaggagc agaagcagcu cgagcggaag cacuacaagg gguggggccg ccuguccgcg     1980

aagcugaucc acggcaucgu ggaccagaag acccagaaga ccauccucga cuaccugauc     2040

gacgacgacg accugccgaa gaaccggaac cgcaacuuca ugcagcucau caacgacgag     2100

aaccugagcu ucaaggagga gaucgagaag aucgccuucg acaacgacaa guccacggag     2160

gagaucgugc aggagcuggc cgggagcccc gccaucaaga agggcauccu ccagucccug     2220

aagaucgucg aggagaucau cgacaucaug ggggagcugc ccaccaacau cgugguggag     2280

auggcccggg agaaccagac caccgcgcag ggcaaccgcg ccagcaaggc ccgcaugaag     2340

uaccucgagg aguccaucaa gaagcugggg agcuccaucc uggaggacga gcccaucagc     2400

aaggacgcca accuccugcg gaacgaccgc cuguuccucu acuaccugca gaacggccgg     2460

gacauguaca ccggcaacga gcuggacauc aacaaccucu ccagcuacga caucgaccac     2520

aucgucccgc agagcuucgu gaaggacgac uccaucgaca accgcgugcu gacgaccagc     2580

uccaugaacc ggggcaagag caacaccguc cccgccgagu ccguggugaa gaagaugcgc     2640

cccaccuggg agcggcugcu cgcgagcggg cugaucucca agaagaaguu cagcuaccug     2700

acgaaggcca ccaacggggg ccucaccgag gaggacaagg cccacuucau ccagcgccag     2760

cuggucgaga cccggcagau cacgaagaac guggcccaga uccugcacca gaaguacaac     2820

aacgagcagc ucuccgagaa gcccgugcgc gucaucaccc ucaagagcgc gcuggccucc     2880

caguuccgga aggacuucag ccucuacaag auccgcgagc ugaacgacua ccaccacgcc     2940

caggacgccu accugaacgg cgugaucgcc caggcgcucc ugaaggugua cccgaagcug     3000

gagcccgagu ucgucuacgg ggaguaccag aaggugucca uccgggcccu caacaaggcc     3060

accgccaaga aggaguucua cagcaacauc augaacuucu ucaccuccga cgagauggug     3120

gccaacgagg agacgggcga gguccugugg aaccgccagc gggacaucaa gaccaucaag     3180

aaggugauga acuaccacca gaugaacauc gugaagaagg ucgagaucca gaccggggcg     3240

uucaccaagg agagcauccu gcccaagggc cccuccaaga agcucaucgc ccgcaagaac     3300

aacugggccc cggugaacua cgggggcgug gacagcccca cggucgccua cagcgugauc     3360

aucacccacg agaagggcaa ggccgcgaag gugguccagc agcugguggg gaucaagauc     3420

cuggagcggc aggccuucga gcaggacgag guggccuucc ucgaggagaa gggcuucauc     3480

caccccaagg uccagcugaa gcugcccaag uacucccucu accaguucgc cgacgggcgc     3540

cggcgccugc uggcgagcgc cgaggagucc cagaagggca accagauggu gcucccggug     3600

caccugaucg agcugcucua ccacgccaag cacgucagcg acuccagcgg gaagucccug     3660

gaguaccuga acgagcaccg gcacgaguuc gccgagcucc uggaggccau ccugcaguuc     3720

accgagcagu acaucgacgc gggcaagaac cagaagaagg ugcgcgaccu cuacgagaag     3780

aaccaggacg ccgacaugcg cgagcuggcc agcuccuuca uccagcugcu ccagcugaac     3840

aagcagggcg cccccgccga cuucaaguuc uucggggaga ccaucccccg gcgccgguac     3900

aagaacacgg cggagaucgu ggacgccacc uucaucaacc agagcaucac cggccuguac     3960

gagacccaga agcgccucgu cuga                                            3984


<210>  1779
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1779
augaccgagc agcagcagcc cuacaacauc ggccuggaca ucgggaccag cuccaucggc       60

ugggccguga cggaccagaa cgacaagcuc cugcacaucc gcgggcacaa cggcaucggg      120

guccggcugu ucaaggaggg cgagagcgcc gcggagcgcc ggggcuuccg caccacccgg      180

cgccggcucu cccgccggcg cuggcggcug cgcuuccugg accggaucuu cgacgccgag      240

cucgccaagg uggacccgaa cuuccuggcc cgccugaagc agagcaacau cagcccccuc      300

gaccccaaca agcagauguu cgggaacauc cuguucgacg acccccaguu cgacgaccag      360

aaguuccacc agcaguaccc gaccaucuac caccuccgga gcgcccucgc gcagcccgac      420

gcgccccagg ccgacgugcg ccugaucuac cuggcccucc accacaugau caaguuccgg      480

ggccacuucc ugaacggggc ggccgucgag aaguuccacg gcgggcagcu gcaccucgag      540

uccgaguucc aggagcugaa ccagaucuuc agcaaccagc agcgcgacca cgugcagcug      600

cagacgucca gccucgacga gguggccacc auccugaagg acaaccagcg gacccccucc      660

gaccgccagc gggagcugag cgcccgccuc uucgucccgg uggacaagac caucgacaag      720

gagaacaaga agcuggccac ggagcugcuc aaggcggugc ugggccugaa ggccaaguuc      780

gacgucaucc ucggcgugga ggccaccgac ccccaggccu ucaagcugca guucuccagc      840

gacgacuucg acgacaagau cgccgagcug gcguccgagc ucaccgacga ggccaacgag      900

auccugaccg ugcugcagcg ccucuacuuc agccugaacc uggccgacau ccucacgaac      960

cggcagacca agcaggucgc cgacuccauc agcaccgcca ugaucaaccg cuacgacgac     1020

cacaagcacc accugaagau gcugaaggag cucgaggccc aggugaccca ggagcagcgg     1080

gacgggcuga agaaggccua caacgaguac aucgacggcg acaagcccac gaucgacacc     1140

uucuacacca acgugaagaa gaaccuggac gacuccgagc ucgccaacca gaucaagagc     1200

cugaucgacc ugcaggccuu caugcccaag cagcgcagca aggagaacgg guccaucccg     1260

caccagcucc accagcggga gcuggaccag cugaucgagg uccagggcaa guacuacccc     1320

uggcugcucg ccgcgaagga caagcuggac gcccugaucg ccuuccgcgu gcccuacuac     1380

gugggcccca ugaucgaccc gaaccagagc gacgaccccc agaacaccaa guucgccugg     1440

augguccgga aggaggcggg ggagaucacg cccuggaacu ucgaggagaa gguggacaag     1500

accuccagcg ccaccaacuu caucaagcgc augaccacga ccgacaccua ccuccugggc     1560

gagcccgugc ugccgaagaa cucccuccug uaccagcagu ucaccguccu gaacgagcuc     1620

aacaagcuga aggugaacgg gaaggcccug acgguggacc agaagaagua ccucuacgac     1680

cacgucugca agaagcagcg gcgcgugacc cugaagaagc ugagcgacug ccucgucgcc     1740

cggggcgacu uccccauggg ggccaucuuc accggcacca cggaccagaa gaacaugaac     1800

aacacccuga agaccuacca ggacuuccag gagaucuucg gcgacgagau cgacaacccc     1860

gaccgccagg uggacuucga gaagaugauc gaguggucca ccaucuucga ggaccgggag     1920

aucuucaagc ugaagcucgc ggagcugaac uggcugacga gcgagcagcu cgagcgcgug     1980

uccaagaagc gguaccaggg guggggccgc cugagccaga agcugcucac ccacaucguc     2040

aacgagaacg gggagcggau caucgaccag cuguggaaca ccaaccagaa cuucauggag     2100

aucaugggcc aggacuccgu gaagcaggcc auccaggagc acaacggggc caacguggcc     2160

cagaccggca ucaacgagac caucgacgag cuguacacga gcccccagaa caagaaggcc     2220

auccgccaga uccuccuggu cguggacgac aucaagaagg cggugggcua cgccccggcc     2280

aacaucauga uggaguucgc ccgggaggac cgcgacgacc accgccugau cgccucccgg     2340

gcgcgccagc ucgagaagac cuaccaggag aucagcaaga acugguucga caacgagucc     2400

gucacccagg agcugaagga ccagaucaag aacaagacca aguucacgga ccggcuguac     2460

cucuacuuca uccagggcgg gaucgaccug uacaccgggc acccccugaa caucgaccag     2520

cucagcuccu acgacaucga ccacauccug ccccagagcu uccugcucga caacucccug     2580

aacaaccgcg ugcugaccag ccaggccgag aaccgggugc acaaggagga ccgccucccc     2640

ggcgagaccg ucgccaacaa gaugcggccg acguggaaga ugaugcgcga gcaggggcug     2700

augacccagc ggaaguacaa caaccugacc cucaagcccg accagaucag caaguucaac     2760

aaccgcggcc aguucaucaa ccggcagcug guggagaccc gccaggugau caagcuggcc     2820

gcggacauca ugucccagaa guaccaggcc gaccagacga aggucgugac cgugaaggcc     2880

aaccucaccc accacaugcg ggagaaguuc aacuucuaca agagccgcaa cgucaacgac     2940

uaccaccacg ccuucgacgc cuaccugacc cuguucgugg gcaacuggcu ccugaaggag     3000

uaccccaagc ugcagccgua cuucguguac ggggacuucg cgaagacggg caccaacaac     3060

cucaaguccu ucaacuuccu guaccgguuc gagcgcgacc acaagcugaa cgacaagggg     3120

gccaucgaca ccgacaagga ccagcagcuc ggcuacauga agcggaucua ccgcuucaag     3180

aagaugcugg ucaccaagca gcuggagacg aacgagggcg cccucuacgg gcagacccug     3240

uaccccgccc ccaagaacga caagaagacc cggaaccuga ucaacagcaa gaagaaccgc     3300

cccuccgaca ucuacggcgg guacagcaac gagaacggcu ccuuccucac ccuggugaag     3360

cugaagaagg ggagcaagua cgacuaccgg cucguggccg ucccgguguc caagaucagc     3420

gagaucaagg aguccgagcu ggcgacgauc gagaaggcca ucgcgcccca guuccugaag     3480

uccuccggca aguaccgcga cuuccaggug guccucagca agauccugau caaccagaag     3540

gugaucgacg acggcaugau cuucaccauc ggguccaaga gcuacaagca caacgccaag     3600

caggucgugc uguccgacaa cagcauccag aucgugagca cccucucccg caucagcgac     3660

aaggagaagc ugaucaccgu guucgacgag aucgucgaca ucacguccaa guaccugccc     3720

ugguacaagg ugaacgacgu ggagaagacc aagaugcugu uccggcagau gcaggacauc     3780

gacgagauga agaagaucau cgacaacagc cugaucggcc ugcacugcaa cgccuccauc     3840

accgaccucg ggaaggucgg ccugagcaag uacugggggc gcuucgugcu gaccgccggc     3900

aucucccuca cgguggacgc ccagcugauc uaccagagcc ccaccggccu guucgagcac     3960

cgggucaagg uguccgaccu cuga                                            3984


<210>  1780
<211>  3990
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1780
augaacaagc agcccuacaa caucggccug gacaucggga ccagcuccgu gggcuggagc       60

gccaccgagc ucgacaaccg ccugcugcac aucaaggggc acaacggcau cgggguccgg      120

cuguucaagg agggcgaguc cgccgcggac cgccggggcu uccgcacgac ccggcgccgg      180

cuggcccgcc ggaaguggcg ccugcggcuc cugaacgaga ucuucgccac cgagaucgcc      240

aagguggacc cgagcuucuu cgcccgccug aagcagucca acgugagccc caaggacccc      300

aacaagacca uguucgggaa cauccucuuc gacgacgaga accuggacga ccagaaguuc      360

caccacgacu acaagacgau cuaccaccuc cggcaggcga ucaucaacca ccccgagcag      420

aaguucgaca uccgccucau cuaccuggcc augcaccaca ucaucaagua ccggggccac      480

uuccugaacc aggccaacgu ccaggacuuc aagggcggga agaucgaccu cgagaagucc      540

uucaaggccc ugaacgacau cuucgagaac caggggcgcg accugcggcu cgugaccgac      600

gaggcgaacc aguacgugaa gguccugagc gacaacgcca agacccgcuc cgaccggaag      660

aaggagcuga gcaagcuccu guacgugccg aucgacaagg acaccgacaa gaacuccaag      720

aaggugacgg gcgagauccu gaaggccauc cucggcaaca aggccaaguu cgacgucauc      780

uucgggaucg agguggagaa ccccaaggag ugggugcuga ccuucaacag cgacgacuuc      840

gacgacaaga ucgagggccu guccagccag augaccgacg aggacuccga gauccuccug      900

auccugaagg agcucuacuu cgcccugaac cugagcgaca uccucaacaa cuccgucacc      960

gacaagaacg cgaagacgcu gagcgaggcc augaucaacc gcuacgacga ccacaagaac     1020

cagcuggcca ugcucaagaa ggugaucgac uccagcgaca agaagcacgc caaggagcug     1080

aaggaggccu acagcgcgua caucgacggg gagaacaaca agaagaucuc cuucgaggac     1140

cugaagaagc ggauccagaa gaaccucgug gacagcgagc ugucccagaa gaucaacgac     1200

cugcucgacc acgacuacuu ccugcccaag cagcgcagca aggagaacgg cgccaucccc     1260

caccagcugc agcagcagga gcucgaccag aucaucgagu cccagaagca guacuacccg     1320

uuccuggccg agcugaaccc caacgagaag cgcagccggc aggccaagua caagcucgac     1380

gagcuggucg ccuuccgcgu guccuacuac gugggcccca uggucgaggu gaccgacagc     1440

aaccaggaga acaacgggaa guucgcgugg augauccgga aggagcaggg cgagaucacc     1500

cccuggaacu ucgacgagaa gguggaccgc accgagacgg ccaccaaguu caucacccgg     1560

augaccacga ccgacaccua ccugaucggg gagccggucc ugcccaagaa cucccuccug     1620

uaccagaagu ucguggugcu gaacgagcuc aacaaccuga agaucaacca ggagaccauc     1680

acgcccggcc agaagcagua ccuguacgag cacgucauga agaagaacaa gcgcgugacc     1740

cucaagaaga ucgccgacgc ccuggucauc aagggggacu accccuacgc cccggaggug     1800

aagggcacca ccgaccagaa gaaccugaac aacggccuca cgaccuacau cgaccuggcg     1860

aagaucuucg ggaacgagcu ggacgacgug gaccgggagg ccgacuucga gaagaucauc     1920

gaguggagca ccaucuucga ggaccagcac aucuucgucc ucaagcugca ggagcugaag     1980

uggcucaccg agaagcagcg caaccaggug gugaagcugc gguaccaggg cugggggaag     2040

cuguccaagg agcuccuggc cggccugguc gacgacaacg ggcagcgcau caucgaccuc     2100

cuguggagcg acaaccagaa cuucaugcag aucgugaacc agaaguccuu caaggagagc     2160

aucgccgacc acaacggcgc gcaccuggag gagaagaacc ucaaggacgu gaucaacgac     2220

cuguacacga gcccccagaa caagaaggcc auccggcagg ucauccuggu gguggacgac     2280

aucaucaacg ccgucggcua cgagcccgcc aacaucauga uggaguucgc ccgcgaggac     2340

uccaacgacc accggcucac caacucccgc agccggcagc uggagaacgu guacaaggac     2400

aucaccaacu ccugguucga caacgacagc gugaaggacg agcuggagga caagaucaag     2460

aacaaggaca aguucaccga ccgccucuac cuguacuuca cgcagggcgg gaaggaccug     2520

uacaccgggg agccccucaa caucgacgac cugagcuccu acgacaucga ccacauccug     2580

ccgcagagcu ucauccucga caacucccug gacaaccggg uccugaccag ccagcgcaac     2640

aaccgggugu ccaaggagga ccagcucccc ggcgagcugu ucgggaccaa gaugcacgcg     2700

uucuggaagc gccugaaggc cgcccaccuc aucagcaaca agaaguacua caaccugacc     2760

cugacgccca acuccaucag caaguucaac aaccagggcc gcuucaucaa ccggcagcuc     2820

guggagaccc gccaggucau caagcuggcc gccgagaucc ugcacaaccg guacuccgag     2880

gagaagggca ccaacaucgu gaccaucaag gcgaaccuca cgcaccacau gcgcgagaag     2940

uucaacuucu acaagaaccg gaacgugaac gacuaccacc acgccuucga cgccuaccug     3000

accgccuucg ucgggaacug gcugcuccag caguacccca agcugaagcc guacuucgug     3060

uacggcgacu ucgccaagac cgagaucaac aaccucaaga gcuucaacau gcucuacaag     3120

uucgagcagg cgcaccagcu gaacgagaag gacgggaaga ucgccaagga caccgacgcc     3180

cugcucggcu acaugaagaa cguguaccac uucaagaaga ugcuggucac gaaggagcug     3240

gagaccaacc acggcgcccu cuucaaccag acccuguacc ccucccccgc gcacgacauc     3300

aagaagcgcc agcugaucaa gcccaaggac aaccggccga ccgacaucua cgggggcuac     3360

acgcacgagg agcgcaagua caugagccuc gugaagguga ucgggaagaa ggacaacgag     3420

uaccggaugg ccaacguccc ccugcugaag aucaacaaga acaacgacau caaggagauc     3480

cucaaguccc aguucagcaa caagaaguuc gagaucgugc ugcccaaggu gcugaagaac     3540

cagcuguucu ccgagaacgg cgcccuguuc gucaucggga gcaagggcua caaguacaac     3600

gccaaccagc uggugcucuc cgaccuggac augaagcugc ucaacgaccu gagcaacaac     3660

aagagcaucg acgaggugac caucaaccag cuguucaaga acaucgucga cuccaccaac     3720

aaguacaugc cgcucuacga gaucaagaac cugaacgagg ugguggagaa guucaacaac     3780

cuggaguuca acgacaagaa gaagaccauc acggacuucc uccuggccac ccacgcgaac     3840

cccaccauga gcaaccugaa gaacaucggc cuccccuacu ucgggcgcuu cauccggaac     3900

accggcauca agcuguccga gaacgccaac gucaucuacc agagccccac ggggcuguuc     3960

ucccgcaaga ucaagaucag cgacuucuga                                      3990


<210>  1781
<211>  3993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1781
augaagaacg acuacaccau cggccuggac aucgggacca acagcguggg cuacuccguc       60

gugacggacg acuacaaggu gaucagcaag aagaugaacg ucuucgggaa caccgagaag      120

aaguccauca agaagaacuu cuggggcgug cgccucuucg agagcgggca gaccgcccag      180

gaggcccgga ugaagcgcac cucccggcgc cggaucgcgc gccggaagaa ccgcaucugc      240

uaccugcagg agaucuucca gcccgagaug aaccaccugg acaacaacuu cuucuaccgg      300

cucaacgaga gcuuccuggu ggccgacgac gccaaguacg acaagcaccc gaucuucggc      360

acgcuggacg aggagaucca cuuccacgag caguucccca ccaucuacca ccuccgcaag      420

uaccuggccg acggcgacga gaaggccgac cugcggcucg ucuaccuggc gaucgcccac      480

aucaucaagu uccgcgggaa cuuccugauc gagggcgagc ucaacaccga gaacaacucc      540

gugaucgagc ugagcaaggu guucguccag cuguacaacc agacccucuc cgagcuggag      600

ggguuccagu ucaucgacga gagcaucgac uucuccgagg ugcugacgca gcagcucagc      660

aaguccgagc gggccgacaa cgugcugaag cuguuccccg acgagaaggg caccgggauc      720

uucgcccagu ucaucaagcu caucgucggc aaccagggca acuucaagaa gguguuccag      780

cuggaggagg accagaagcu gcagcucagc accgacgacu acgaggagaa caucgagaac      840

cugcuggcga ucaucgggga cgaguacggc gacaucuucg uggccgccca gaaccucuac      900

caggccaucc ugcuggccgg gauccucacc uccacggaga agacccgcgc gaagcugagc      960

gccuccauga uccagcggua cgaggagcac gccaaggacc ugaagcuccu gaagcgcuuc     1020

gucaaggagc acauccccga caaguacgcc gagaucuuca acgacgccac caagaacggc     1080

uacgcgggcu acaucgacgg gaagaccaag gaggaggagu ucuacaagua ccugaagacg     1140

acccucgugc agaaguccgg cuaccaguac uucaucgaga agaucgagca ggagaacuuc     1200

cugcggaagc agcgcaucua cgacaacggg gugaucccgc accaggucca cgccgaggag     1260

cugcgggcca uccuccgcaa gcaggagaag uacuacagcu uccugaagga gaaccacgag     1320

aagaucgagc agaucuucaa ggugcggauc cccuacuacg ugggcccccu ggccaagcac     1380

aacgagcaga gccgcuucgc cuggaacauc cgcaagagcg acgagcccau ccggccgugg     1440

aacaugaacg acgucgugga cgagaacgcg uccgccgugg ccuucaucga gcgcaugacc     1500

aucaaggaca ucuaccucaa cgagaacguc cugccccggc acagccugau cuacgagaag     1560

uucaccgugu ucaacgagcu cacgaagguc cuguacgccg acgaccgcgg gguguuccag     1620

cgguucuccg ccgaggagaa ggaggacauc uucgagaagc uguucaagag cgagcgcaag     1680

gugaccaaga agaagcucga gaacuaccug cggaucgagc uguccaucag cucccccagc     1740

gucaagggca ucgaggagca guucaacgcg aacuucggca ccuaccugga ccucaagaag     1800

uucgacgagc ugcaccccua ccuggacgac gagaaguacc aggacacccu cgaggaggug     1860

aucaaggugc ugacggucuu cgaggaccgc uccaugaucc agaaccagcu ggagcagcuc     1920

ccgcugaacc ugagcaccaa gaccaucaag gcccucuccc ggcgcaagua caccgggugg     1980

ggccggcuga gcgcccgccu gaucgacggg auccacgaca agaacuccgg caagacgauc     2040

cucgacuacc ugaucgagga cgagagcgac uccuacaucg ugaaccggaa cuucaugcag     2100

cugaucaacg acgaccaccu cagcuucaag aagaucaucg aggacuccca gcccuacaag     2160

gagcagcaga gcgccgagga gaucgugucc gagcugagcg ggagccccgc gaucaagaag     2220

ggcauccugc agucccucaa gaucgucgac gagcuggugg ccaucauggg cuacaagccc     2280

aagaacaucg uggucgagau ggcccgcgag aaccagacca ccgggcgggg caagcagaac     2340

agcaagccgc gccugaaggg gaucgagaac ggccucaagg aguucuccga cagcgugcug     2400

aaggggucca gcaucgacaa caagcagcug cagaacgacc ggcucuaccu guacuaccug     2460

cagaacggca aggacaugua caccggccac gagcucgaca ucgaccaccu guccacguac     2520

gacaucgacc acaucauccc ccagagcuuc cugaccgaca acuccaucga caaccgcgug     2580

cucaccacca gcaaguccaa ccggggcaag agcgacaacg uccccuccga ggagguggug     2640

cgcaagaugg accgcuucug gcggaagcug cugaacgcca agcucaucag cgagcgcaag     2700

uacacgaacc ugaccaagaa ggagcugacc gaguccgaca aggccggguu ccucaagcgg     2760

cagcuggucg agacccgcca gaucaccaag cacguggcga cgauccugga cagcaaguuc     2820

aacgaggacu ccaacaaccg ggacgugcag aucaucaccc ucaagagcgc ccuggucagc     2880

gaguuccgca agaccuucaa ccuguacaag gugcgggaga ucaacgaccu ccaccacgcc     2940

cacgacgccu accugaacgc cguggucgcg cugucccucc ugcgcgugua cccccagcug     3000

aagccggagu ucguguacgg ggaguacggc aagaacagca uccacgacca gaacaaggcc     3060

accaucaaga agcaguucua cuccaacauc acgcgguacu ucgccagcaa ggacuacauc     3120

aucaacgacg acggcgagau ccucuggaac aagcaggaga ccaucgccca ggucaucaag     3180

acccugggga ugcaccaggu gaacgugguc aagaaggugg agauccagaa gggcggguuc     3240

uccaaggaga gcauccagcc caagggcgag ucccagaagc ugauccgccg gaagcagcag     3300

uggaacacca agaaguacgg gggcuucgac agccccgugg ucgccuacgc gauccuccug     3360

uccuucgaca agggcaagcg caaggcccgg agcuucaaga ucguggggau cacgauccag     3420

gaccgcgagu ccuucgaggg caaccccauc cuguaccuca gcaagaagga cuaccacaac     3480

ccgaaggugg aggccauccu gcccaaguac ucccuguucg aguucgagaa cgggcggcgc     3540

cggauggucg ccagcgcguc cgagacccag aagggcaacc agcucaucau ccccgggcac     3600

cugauggagc ugcucuacca cagcaagaag aucaucaacg gcaagaacuc cgacagcgug     3660

uccuacaucc agaacaacaa ggagaaguuc cgcgagaucu ucgaguacau cguggacuuc     3720

agcagcaagu acaucuccgc cgacgccaac cugaacaaga ucgagaagau cuucgagaac     3780

aacuuccaca aggccagcga gcaggagauc gccaaguccu ucaucaaccu gcucaccuuc     3840

accgcgaugg gcgcccccgc cgacuucgag uucuucgggg agaagauccc gcggaagcgc     3900

uacgucagca ucuccgagau caucgacgcc guguucaucc accagagcau cacgggccug     3960

uacgagaccc gggugcgccu gaccgagguc uga                                  3993


<210>  1782
<211>  3993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1782
auggagaagg aguacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacggacg acuaccgccu gguggcccgg aagaugucca uccaggggga cagcaaccgc      120

aagaagauca agaagaacuu cuggggcgcg cggcuguucg aggaggggaa gaccgcccag      180

uuccgccgga ucaagcgcac caaccggcgc cggaucgccc gccggcgcca gcgggugcuc      240

gcccugcagg acaucuucgc cgaggagauc cacaagaagg accccaacuu cuucgcgcgc      300

cuggaggagg gcgaccgggu cgaggccgac aagcgcuucg ccaaguuccc gguguucgcc      360

acccucuccg aggagaagaa cuaccaccgg caguacccca cgaucuacca ccuccgccac      420

gaccuggcga acagcaagga gcaggccgac auccggcucg uguaccuggc caucgcccac      480

ugccugaagu accgcggcca cuuccucuuc gagggggagc uggacaccga gaacaccucc      540

gucaccgaga acuaccagca guuccugcag gccuaccagc aguucuuccc cgagcccauc      600

ggcgaccucg acgacgcggu gccgauccug acggagcggc ugagcaaggc caagcgcgug      660

gagaaggucc ucgccuacua ccccuccgag aaguccaccg ggaacuucgc ccaguuccug      720

aagcugaugg ugggcaacca ggccaacuuc aagaagaccu ucgaccucga ggaggagaug      780

aagcugaacu ucacccgcga cugcuacgag gaggaccuga acgagcuccu ggagaagacg      840

uccgacgacu acgcggagcu guuccucaag gccaaggggg uguacgacgc cauccugcug      900

agccagaucc ucuccaagag cgacgacgag accaaggcca agcuguccgc caacaugaag      960

cugcgguucg aggagcacca gcgcgaccuc aagcagcuga aggagcuggu ccggcgcgac     1020

cuccccaaga aguacgacga cuucuucaag aaccggagca agaacggcua cgcgggcuac     1080

gugaagggga aggccaccca ggaggacuuc uacaaguucc ugcgcaccga gcuggccggc     1140

cucgaggagu cccagagcau cauggagaag aucgaccugg agaucuaccu gcucaagcag     1200

cggacguucg ccaacggggu gaucccccac cagauccacc uggucgagau gcgcgagauc     1260

auggaccggc agaagcgcuu cuacccguuc cugaagggcg cccagggcaa gaucgagaag     1320

cuccugaccu uccggauccc cuacuacgug gggccccugg cgcaggaggg ccaguccccc     1380

uucgccugga ucaagcgcaa gagcccgucc cagaucaccc ccuggaacuu cgccgaggug     1440

gucgacaagg agaacagcgc caucgaguuc aucgagcgga ugaccaacca ggacacguac     1500

cuccccaagg agaaggugcu gcccaagcag agccugaucu accagcgcuu caugaucuuc     1560

aacgagcuga ccaagguguc cuacaccgac gagcggggca agagccacua cuucuccagc     1620

gagcagaagc gcaagaucuu caacgagcug uucaagcagc acccgcgggu caccgagaag     1680

cagcugcgca aguuccugga gcucaacgag cagaucgacu ccacggagau caaggggauc     1740

gagaccagcu ucaacgcguc cuacagcacc uaccacgacc ugcugaagcu cuccgaccag     1800

auggacaccc ugcuggacga ccccgacaug acgaccaugu ucgaggagau caucaagauc     1860

cucaccaucu ucgaggaccg ggagaugauc cgcgagcagc ugaagcccua cgagaccgug     1920

cuggggcucc ccgccaucaa gaagcuggcc aagaagcacu acacgggcug gggccgccug     1980

agcgagaaga ugauccaggg gaugcgggag aagcaguccc gcaagaccau ccucgacuac     2040

cugaucgacg acgacgacuu cccgugcaac cggaaccgca acuucaugca gcugaucaac     2100

gacgaccacc ucagcuucaa ggagaccauc gccaacgagc ugaucauguc cgacagcaac     2160

gugcugcucg accaggucaa ggccaucccc ggcucccccg cggugaagaa ggggaucugg     2220

cagagcauca agaucgucga ggagaucauc ggcaucaucg ggaaggcccc caagaacauc     2280

gugaucgaga uggcccggga gaaccagcgc accucccgga gccgcccgcg gcugaaggcc     2340

cuggaggagg cccucaagaa caucgacagc ccccugcuga aggacuaccc cacggacaac     2400

caggcgcucc agaaggaccg ccuguaccug uacuaccucc agaacggcaa ggacauguac     2460

accggcgagc cccuggagau ccaccggcug agcgaguacg acaucgacca caucaucccg     2520

cgcagcuuca ucguggacaa cucccucgac aacaaggucc uggugagcuc caaggugaac     2580

cgggggaagc uggacaacgc ccccgacccc cucgucguga agcgcaugcg gagccacugg     2640

gagaagcugc accaggccaa gcugaucucc gacaagaagc ucgccaaccu gaccaagcag     2700

aaccugaccg aggccgacaa ggcgcgcuuc auccagcggc agcucgugga gacccgccag     2760

aucacgaagc acgucgccaa ccugcugcac cagcacuuca accucccgga ggaggugagc     2820

gccaccgaga agaccuccau caucacccuc aagagcacgc ugaccuccca guuccggcag     2880

auguucgaca ucuacaaggu gcgcgagauc aaccaccacc accacgccca cgacgcguac     2940

cugaacggcg ucguggccau gacccugcug aagaaguacc ccaagcucgc ccccgaguuc     3000

guguacggga gcuacaucaa gggcgacauc aaccagauca acaaggccac cgccaagaag     3060

gaguucuacu ccaacaucau gaaguucuuc gagagcgagg agaucaucug cgacgagcag     3120

ggggagguca ucuggaacaa gaagcgggac cuguccacga ucaagaagac caucggcgcg     3180

caccagguga acaucgugaa gaaggucgag aagcagaagg gcggguucua caaggagacc     3240

aucaacagca aggccaaccc cgagaagcug aucccgcgca aggccucccu cgacccccug     3300

aaguacggcg gguacggcag cccccuggug gccuacaccg ugaucuucau cuucgagaag     3360

ggcaagcaga agaaggucac gaaggggauc gagggcauca ccgugaugga gcagcuccgc     3420

uucgagcagg acccccggga guuccugaag accaaggggu acgagggcgu gaagcagugg     3480

cugauccucc cgaaguacau ccuguucgag gcccagggcg gguaccgccg gaugaucgcg     3540

ucccaccagg agacccagaa ggccaacagc cugauccucc ccgagaaccu ggucacgcug     3600

cucuaccacg cccgccacua cgacgagauc aaccacaagg ugagcuucga cuacgugaac     3660

gcccacaagg agggcuucaa cgacaucuuc gacuucaucu ccgacuucgg gguccgguac     3720

auccuggcgc cccagcaccu ggagaagauc aagguggccu acgagaagaa caaggaggug     3780

gaccucaagg agaugaucga cgccauccug agccugcuca aguucacccu guucggcgcc     3840

uccgucgagu ucaaguucuu cgacaucaag auccugaagc gcuacaagag ccucaccgac     3900

aucugggagg ccaccaucau cuaccagucc gugacggggc uguacgagcg gcgcguggag     3960

guccggaagc ugugggacgg cgagcgccuc uga                                  3993


<210>  1783
<211>  3996
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1783
augaacaagc agcccuacaa caucggccug gacaucggga ccagcuccgu gggcuggagc       60

gccaccgagc ucgacaaccg ccugcugcac aucaaggggc acaacggcau cgggguccgc      120

cucuucaagg agggcgaguc cgccgcggac cggcggggcu uccgcacgac ccggcgccgg      180

cuggcccgcc ggaaguggcg ccugcggcuc cugaacgaga ucuucgccac cgagaucgcc      240

aagguggacc cgagcuucuu cgcccgccug aagcagucca acgugagccc caaggacccc      300

aacaagacca uguucgagaa cauccucuuc gacgacgaga accuggacga ccagaaguuc      360

caccacgacu acaagacgau cuaccaccuc cggcaggcga ucaucaacca ccccgagcag      420

aaguucgaca uccgccucau cuaccuggcc augcaccaca ucaucaagua ccgggggcac      480

uuccugaacc aggccaacgu ccaggacuuc aagggcggga agaucgaccu cgagaagucc      540

uucaaggccc ugaacgacau cuucgagaac cagggccgcg accugcggcu cgugaccgac      600

aaggcgaacc aguacgugaa gguccugagc gacaacgcca agacccgcuc cgaccggaag      660

aaggagcuga gcaagcuccu guacgugccg aucgacaagg acaccgacaa gaacuccaag      720

aaggugacgg gggagauccu gaaggccauc cucggcaaca aggccaaguu cgacgucauc      780

uucggcaucg agguggagaa ccccaaggag ugggugcuga ccuucaacag cgacgacuuc      840

gacgacaaga ucgaggggcu guccagccag augaccgacg aggacuccga gauccuccug      900

auccugaagg agcucuacuu cgcccugaac cugagcgaca uccucaacaa cuccgucacc      960

gacaagaacg cgaagacgcu gagcgaggcc augaucaacc gcuacgacga ccacaagaac     1020

cagcuggcca ugcucaagaa ggugaucgac uccagcgaca agaagcacgc caaggagcug     1080

aaggaggccu acagcgcgua caucgacggc gagaacaaca agaagaucuc cuucgaggac     1140

cugaagaagc ggauccagaa gaaccucgug gacagcgagc ugucccagaa gaucaacgac     1200

cugcucgacc acgacuacuu ccugcccaag cagcgcagca aggagaacgg ggccaucccc     1260

caccagcugc agcagcagga gcucgaccag aucaucgagu cccagaagca guacuacccg     1320

uuccuggccg agcugaaccc caacgagaag cgcagccggc aggccaagua caagcucgac     1380

gagcuggucg ccuuccgcgu gcccuacuac gugggcccca uggucgaggu gaccgacucc     1440

aaccaggaga acaacggcaa guucgcgugg augauccgga aggagcaggg ggagaucacc     1500

ccguggaacu ucgacgagaa gguggaccgc accgagacgg ccaccaaguu caucacccgg     1560

augaccacga ccgacaccua ccugaucggc gagcccgucc ugcccaagaa cagccuccug     1620

uaccagaagu ucguggugcu gaacgagcuc aacaaccuga agaucaacca ggagaucauc     1680

acccccgggc agaagcagua ccuguacgag cacgucauga agaagaacaa gcgcgugacg     1740

cucaagaaga ucgccgacgc ccuggucauc aagggcgacu acccguacgc ccccgaggug     1800

aaggggacca ccgaccagaa gaaccugaac aacggccuca ccacguacau cgaccuggcg     1860

aagaucuucg gcaacgagcu ggacgacgug gaccgggagg ccgacuucga gaagaucauc     1920

gaguggucca ccaucuucga ggaccagcac aucuucgccc ucaagcugca ggagcugaag     1980

uggcucaccg agaagcagcg caaccagguc gugaagcugc gguaccaggg guggggcaag     2040

cugagcaagg agcuccuggc cgggcuggug gacgacaacg gccagcgcau caucgaccuc     2100

cugugguccg acaaccagaa cuucaugcag aucgucaacc agaaguccuu caaggagagc     2160

aucgcggacc acaacggggc ccaccuggag gagaagaacc ucaaggacgu gaucaacgac     2220

cuguacacca gcccccagaa caagaaggcc auccgccagg ugauccuggu cguggacgac     2280

aucaucaacg ccgugggcua cgagcccgcc aacaucauga uggaguucgc gcgggaggac     2340

uccaacgacc accggcucac gaacagccgc ucccggcagc uggagaacgu cuacaaggac     2400

aucaccaaca gcugguucga caacgacucc gugaaggacg agcuggagga caagaucaag     2460

aacaaggaca aguucaccga ccgccucuac cuguacuuca cccagggcgg gaaggaccug     2520

uacacgggcg agccgcucaa caucgacgac cugagcagcu acgacaucga ccacauccug     2580

ccccaguccu ucauccucga caacagccug gacaaccggg ugcugaccuc ccagcgcaac     2640

aaccggguca gcaaggagga ccagcucccc ggggagcugu ucggcaccaa gaugcacgcc     2700

uucuggaagc gccugaaggc cgcccaccuc aucuccaaca agaaguacua caaccugacc     2760

cugaccccca acagcaucuc caaguucaac aaccaggggc gcuucaucaa ccggcagcuc     2820

guggagacgc gccaggugau caagcuggcc gcggagaucc ugcacaaccg guacagcgag     2880

gagaagggca ccaacaucgu caccaucaag gccaaccuca cccaccacau gcgcgagaag     2940

uucaacuucu acaagaaccg gaacgugaac gacuaccacc acgccuucga cgccuaccug     3000

acggccuucg ugggcaacug gcugcuccag caguacccga agcugaagcc cuacuucguc     3060

uacggggacu ucgcgaagac cgagaucaac aaccugaagu ccuucaacau gcucuacaag     3120

uucgagcagg cccaccagcu gaacgagaag gacggcaaga ucgccaagga cgccgacgcg     3180

cugcucgggu acaugaagaa cguguaccac uucaagaaga ugcuggugac caagaagcug     3240

gagaccaacc acggcgcccu cuucaagcag acgcuguacc ccagccccgc ccacgacauc     3300

aagaagcgcc agcugaucaa gccgaaggac aaccggccca ccgacaucua cggcggguac     3360

acccacgagg agcgcaagua caugucccuc gucaagguga ucggcaagaa ggacaacgag     3420

uaccggaugg ccaacgugcc ccugcugaag aucaacaaga acaacgacau caaggagauc     3480

cucaagagcc aguucuccaa caagaaguuc gagaucgucc ugccgaaggu gcugaagaac     3540

cagcuguuca gcgagaacgg ggcccuguuc gugaucggcu ccaaggggua caaguacaac     3600

gcgaaccagc ugguccucag cgaccuggac augaagcugc ucaacgaccu guccaacaac     3660

aagagcaucg acgaggugac caucaaccag cuguucaaga acaucgugga cagcacgaac     3720

aaguacaugc cccucuacga gaucaagaac cugaacgagg ucguggagaa guucaacaac     3780

cuggaguuca acgacaagaa gaagaccauc accgacuucc uccuggccac ccacgccaac     3840

cccacgaugu ccaaccugaa gaacaucggc cuccccagcu acuucggccg cuucaccucc     3900

gggaacgccg gcaucaagcu gagcgagaac gccaacguga ucuaccaguc cccgaccggg     3960

cuguucagcc ggaaggucaa gaucuccgac uucuga                               3996


<210>  1784
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1784
augaagaacc gcgagaacau caaguacuac aucggcgugg acaucgggac caacagcguc       60

ggcugggccg ugaucgacga gaacgggaac cugcucaaga agggcaagca ccaccugugg      120

gggucccggc uguucgacca ggcccagacc gcgcagaacc gccggaacua ccgcagcucc      180

cggcgccggu acaacaagcg ccggcagcgc aucggccucc ugcggcugau caugagcgac      240

auggugcucg aggucgaccc cuccuucuuc auccgccugg agaagacgac cuuccuggac      300

aaggaggaca agaaggccau ccucaaggac aacuacaaga ugaacuacaa ccuguucugc      360

gacgaggacu acaacgacaa gaaguacuuc aaggacuacc cgaccaucua ccaccuccgg      420

aagaagcucu gcgagagcga cgagaaggcc gacccccgcc ugaucuaccu ggcccuccac      480

cacaucguga aguaccgggg caacuuccug uacgaggggc aggagcugca ccucgagccc      540

uccaacaagg aggaggaccu gaagauccug uucgacaucc ucggcaagaa caacgacacc      600

guguacgaca ucagcgagga gcagauccag uucauccuga agacggucgu ggagaacauc      660

uccaagaccg ccaaggugga cgagugcaug agccagcuga agcucaacuc cgaggacaag      720

aagaucguca aggaguucau gcgcgggcug gugggcaaca aguucaacgu gagcaagcug      780

uacaugcacg aggaccucca guucgacgac gaggaccuga agcugcaguu cuccgacaag      840

agcuacgagg agaagaucac cgaguacgag aacguccucg aggagaagau ggaguucauc      900

gaccugaugc agcgguucua cuccuggauc gagcugagca agaucguggg guccgacagc      960

cagcacgcgu ccaucagcgg cgccauggug aacaucuacg agagccaccg cgaggaccuc     1020

cggacccuga aggaggucau gcugaagauc ggcaagaagg aguacgacga gauguucaag     1080

cccacgucca agaacguggu gaacuacuac aacuacguca acccgguggc cugcagcggg     1140

gacaagaccg acggcuucua caaguacgug aagaaggcca ucgagaaguc cgacgacagc     1200

cgcaaggacg agauccucca gaagaucgcg aacgagaccu acaugcugaa gcagaccucc     1260

aagaacaacg ccuacauccc cuaccagaug cagaaggacg agcugaucaa gauccucgac     1320

caccaggaga aguacuaccc cguccugaag gagaaccggg acaagaucau cagcauccug     1380

gaguuccgca uccccuacua cuacgggccg cucgacggca acaagcaguu cggcuggcug     1440

aucaagaaga aggggaagga gaacgagcgc auccugcccu ggaaccacca ggagaucgug     1500

gacgugcagg agacggccgc ccaguucauc aagaagcuca ccaacuacug caccuaccug     1560

cccaucgaga aggucaugcc ccagaagucc cugaccugca gcauguacga ggugcucucc     1620

gaggucaaca agauccggau cgacggcaag cugcugccga ucgacacgaa gaaccgccug     1680

aucgaggacc ucuucuucaa gcggaagacc gugaaggaga aggaccugau caacuggcug     1740

aagcagaacc agcucaccgu gggggagauc accggcuacc agaaggagaa ggccuucagc     1800

uccagccugg cgcccuggau cgacuucaag gagaucuucg acgagaucaa cgacuccaac     1860

uacgaccuga ucgagaagau caucgaggac augacgaucu ucaacgaggg gagcauccuc     1920

aaggagcgcc ugaagaaggc cuacaaccug gaccagaaca aggucaagaa gaucaugaag     1980

cucaaguacu ccggcuggag ccggcugucc aagaagcuga ucaacggcau ccgcgccgac     2040

aacaaguucg ggagcuccgu gagcauccuc gacgugauga aggagagcca cauggcccug     2100

auggagauca ucaacgacca ggaccugggc uucaagcaga ucaucgagaa ggagaacuuc     2160

aagaacgaga ccggguccuu cgaguacgag gacaucgaga accucgccgg cagccccgcg     2220

cugaagcggg ggaucuggca gacccugcag gucaucgagg agaucaagaa cuacaugggc     2280

cacgagccca agaacaucua caucgaguuc gcccgcgagg agcaggagaa ggugcggacc     2340

acgacccgcg ugaagaagcu caaguccauc uacaacgaca ucaagaacca gcuggacguc     2400

cacggcaagg agguguacag caaccugaac aagcaggacg agaaguccag caucgagaag     2460

cggcucuacc uguacuacac ccagcugggg aagugcaugu acuccggcga gagccucgac     2520

aucgacaagc uguccaacua cgagaucgac cacaucuucc cgcgcacccu gacgagcgac     2580

gacucccucg acaaccuggu gcuggucaag aagaaggaga accagcggaa gcucgacgac     2640

cuggugcugc cccucgagau ccgcaacaag auggaggugu ucuggaagaa gcuguacgac     2700

aacgggcuga ucacccagac caaguacuac cggcucaugc gcgacgaguu ccggcgcgac     2760

cagaucgaca aguucaucaa ccggcagcug gucgagaccc gccagaucau caagcacgug     2820

gccaacauca ucgagaacca cuacgaggac acgaaggugu ucaccguccg cgccaaccug     2880

agccacgagu uccgggagaa guacggcauc uacaagaacc gcaacgugaa cgacuuccac     2940

cacgcccacg acgcguacau cgccugcauc aucgggcggu acauccagau ccgcuucccc     3000

ggccucgagg ccaaguacgu guacggccag uacaugcaga acgucaagaa gaccaagaac     3060

aacaucgaca aggagaacca cggguucauc aucaacucca ugaaguacga gugcaucgac     3120

gaggacaccg gcgaggugau cuggaacccc gagcggaucc uggacuacau caagugcuuc     3180

aacuaccgcg acguguacau cacgaagaag cuggacacca acaaccggga gcuguucaac     3240

gucaccaucc ugccgaacga caagaacagc gagaagggga agaccaaggc caccaucccc     3300

gugaacaagc ugcgcuccaa cgugcggaag uacggcggcu ucagcgggcu ccaguccgac     3360

aucgucgcca ucgagggcaa gaaggggaag aagaucgacc gccggcugau caaccugccc     3420

auccuccugc gcuacgugag caucgaggac cagugcaagu acaucaugga gaacaacaag     3480

uacagcgacg ugaagaucau caagaagauc aagaagaacc agcugaucga gaucgacggc     3540

aucugcuacu accucaaguc cgcggacgag cuggacacgg ccuaccagcu gauccucagc     3600

cgggacgaca acgagaugau cuaccgcauc gaccgggcca uccagaacaa cgacuucucc     3660

uacuucgaca acaagaagga gaacaucgac gagcuguacc gccugaucug ccacaagaug     3720

aucgagaacu accccaagua caagaagaag gucuacgagu ucaucaagga gcgggaggag     3780

gaguucuucg agcucgacaa cagccagaag ugcaucguga ucuucgagau gauccgcgug     3840

augaagcgcg gggagcagua caucaacauc ccguucgagc ccuacaaccu gaagaacgac     3900

cggaagggcc ggcugaacug ccaguccauc cuccuggaca acaccuacuu cuacgacguc     3960

agcaucaccg gcaucuacuc caagaaguac aagcuguga                            3999


<210>  1785
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1785
augcagaaga acuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

augaaggacg acuacacgcu cauccgcaag cggaugaagg ugcuggggaa caccgacauc      120

aagaagauca agaagaacuu cuggggcgug cgccuguucg acgaggggga gaccgccaag      180

gagacccggc ucaagcgcgg cacgcggcgc cgguaccagc gccggcgcaa ccggcugauc      240

uaccugcagg acaucuucca gcagcccaug cucgcgaucg acgagaacuu cuuccaccgc      300

cuggacgacu ccuucuucgu cccggacgac aagagcuacg accggcaccc caucuucggc      360

ucccuggagg aggagguggc cuaccacaac accuacccca ccaucuacca ccuccgcaag      420

caccuggccg acaaccccga gaaggccgac cugcggcucg uguacaccgc ccuggcgcac      480

aucgucaagu accgcgggca cuuccugauc gagggcgagc ucaacacgga gaacaccagc      540

aucuccgaga ccuucgagca guuccuggac accuacagcg acaucuucaa ggagcagcug      600

gugggggaca ucuccaaggu ggaggagauc cucagcucca agcagagccg gucccgcaag      660

cacgagcaga ucauggcccu guucccgaac gagaacaagc ugggcaacuu cgggcgguuc      720

augaugcuca ucgucggcaa cacgagcaac uucaagcccg uguucgaccu ggacgacgag      780

uacaagcuga agcucuccga cgagaccuac gaggaggacc uggacacccu gcucggcaug      840

accgacgacg uguuccugga cgucuucaug gccgccaaga acguguacga cgcgguggag      900

augagcgcca ucaucuccac ggacaccggg aacagcaagg ccguccuguc caaccagaug      960

aucaacuucu acgacgagca caagguggac cucgcccagc ugaagcaguu cuucaagacc     1020

caccuccccg acaaguacua cgagugcuuc agcgaccccu ccaagaacgg cuacgccggg     1080

uacaucgacg gcaagaccaa ccaggaggac uucuacaagu acaucgagaa ggugaugaag     1140

acgaucaaga gcgacaagaa ggacuacuuc cucgacaaga ucgaccgcga ggucuuccug     1200

cggaagcagc gcagcuucua caacuccgug aucccgcacc agauccaccu ccaggagaug     1260

caggcgaucc uggaccgcca gagccaguac uaccccuucc uggccgagaa ccgggacaag     1320

aucgaguccc uggugaccuu ccgcaucccc uacuacgucg gcccccucac cgugagcgac     1380

caguccgagu ucgccuggau ggagcggcag agcgacgagc cgauccgccc cuggaacuuc     1440

gacgagaucg ugaacaagga gcgguccgcc gagaaguuca ucgagcgcau gaccaacaug     1500

gacacguacc ugcuggagga gaagguccuc cccaagcgga gccugcugua ccagaccuuc     1560

gagguguaca acgagcucac caagguccgc uacaccaacg agcaggggaa gacggagaag     1620

cugaaccggc agcagaaggc cgagaucauc gagacccugu ucaagcagaa gaaccgcgug     1680

cgggagaagg acaucgcgaa cuaccucgag caguacggcu acguggacgg gaccgacauc     1740

aagggcgucg aggacaaguu caacgccucc cugagcaccu acaacgaccu ggccaagauc     1800

gacggggcca aggccuaccu ggacgacccc gaguacgcgg acguguggga ggacaucauc     1860

aagauccuca cgaucuucga ggacaaggcc augcgcaaga agcagcugca gaccuacucc     1920

gacacccuga gcccggagau ccucaagaag cuggagcgga agcacuacac cggcuggggc     1980

cgcuucucca agaagcugau caacgggcuc cgggacgagg gcagcaacaa gacgauccug     2040

gacuaccuga aguccgacga ggggagcucc ggccccacca accgcaacuu caugcagcuc     2100

auccgggaca acacccugag cuucaagaag aagaucgagg acgcccagac caucgaggac     2160

accacgcaca ucuacgacac cguggccgag cugcccgggu cccccgccau caagaagggc     2220

auccgccagg cgcucaagau cgucgaggag aucaucgaca ucaucggcua cgagccggag     2280

aacaucgugg uggagauggc ccgggagagc cagaccacca agaaggggaa ggaccugagc     2340

aaggagcgcc uggagaagcu cacggaggcc aucaaggagu ucgacggccc cuccgacguc     2400

aaggugaagg accugaagaa cgagaaccug cgcaacgacc ggcucuaccu guacuaccug     2460

cagaacgggc gcgacaugua caccaacgag ccccucgaca ucaacaaccu gagcaaguac     2520

gacaucgacc acaucauccc ccaguccuuc accaccgaca acagcaucga caacaaggug     2580

cuggucuccc ggacgaagaa ccagggcaac aagagcgacg acgugccguc caucaacauc     2640

gugcacaaga ugaagcccuu cuggcgccag cuccacaagg ccgggcugau cagcgaccgg     2700

aaguucaaga accugaccaa ggcggagcac ggcggccuca ccgaggccga ccgcgcccac     2760

uuccugaacc ggcagcuggu cgagacccgc cagaucacga agcacguggc caaccuccug     2820

gacucccagu acaacaccgc cgaggagcag cggaucaaca ucgugcugcu caagagcucc     2880

augaccagcc gcuuccggaa ggaguucaag cuguacaagg uccgcgagau caacgacuac     2940

caccacgggc acgacgcgua ccugaacgcc gugguggcca ccacgaucau gaaggucuac     3000

cccaaccuca agccccaguu cguguacggc caguacaaga agaccuccau guucaaggag     3060

gagaaggcca ccgcccggaa gcacuucuac agcaacauca ccaaguucuu caagaaggag     3120

aaggugguca acgaggagac gggggagauc cugugggaca ccgagcgcca ccuguccacc     3180

aucaagcggg ugcucagcug gaagcagaug aacaucguga agaaggucga gaagcagaag     3240

ggccagcugu ggaaggagac caucuacccg aagggcgacu ccagcaagcu gauccccgug     3300

aaggagggga uggaccccca gaaguacggc gggcucagcc agguguccga ggcguucgcc     3360

gucgugauca cgcacgagaa gggcaagaag aagcagcuca agagcgaccu gaucuccauc     3420

ccgaucgugg accagaaggc cuacgagcag caccccaccg ccuaccugga ggaggccggg     3480

uacaacaacc ccaccguccu gcacgagcug uucaaguacc agcuguucga gcuggaggac     3540

ggcagccgcc ggaugaucgc guccgccaag gaguuccaga agggcaacca gauggugcug     3600

ccccucgagc ugguggagcu gcucuaccac gccaaccgcu acgacaaggu caaguucccg     3660

gacagcaucg aguacgugca cgacaaccug gccaaguucg acgaccugcu cgaguacgug     3720

aucgacuucu ccaacaagua caucaacgcg gacaagaacg uccagaagau ccagaagauc     3780

uacaaggagc acgggaccga ggacguggag cugacggugg agagcuucgu caaccugaug     3840

accuucaccg ccaugggcgc ccccgccacc uucaaguucu acggggaguc caucacgcgg     3900

agccgcuaca ccuccaucac cgaguuccgg ggcagcaccc ucaucuucca guccaucacg     3960

gggcuguacg agacccgcua caagcuggag gacaacuga                            3999


<210>  1786
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1786
augaugaagg aguacagcau cggccuggac aucgggucca gcuccguggg cuucgucgcc       60

guggacgacc aguaccgcgu gguccgggcc aaggggaaga acgugaucgg cacccgccuc      120

uucgacgagg ggaagaccgc ggccgaccgg cgcggccugc ggacgacccg ccggcgccug      180

agccggcgca aguggcggcu ccgccugcug cggggcaucu ucgagcccca caucaacgag      240

aucgacgagu ccuucuucau gcgccucaag gagagcaaca ucuccccgaa ggacagcaac      300

aagcacuucc ccggggacau ccuguucaac gaccgguccg accgcgaguu cuacgagaac      360

uaccccacca ucuaccaccu ccggcaggcc cucaagaccg agaagcgcca guucgacauc      420

cgggagaucu accuggccau gcaccacauc gugaaguacc gcggccacuu ccuggagggc      480

gggacgguca ccgacuucga ggugggggag cucgaccugg ccggcaaguu cagccggcug      540

aacgagcugu ucgaguccau caaccuggag gugcccuucg uccugcgccu cgacaaccug      600

gaggacguga aggcggugcu gaucgacagc cagcgcuccg cccaggaccg gcagcgcacc      660

cucaccccgg acaucuacgu ccccagcacg gacaagauca ccgagaagcg guucaagggc      720

aucgccaccg agcugcugaa ggccaucgug gggaacaagg cgaaguucga cgugcucgcc      780

ggccuggagc uggacaagga ggaggccaag cucugggccc ugaccuucga cuccgagacg      840

uucgacgagg acaucgagca gcuggagggg gacaugaccg aggccaccca ggaccucauc      900

gagauccuca agagccugcg cucccucauc cggcuggcgg ucaacaugcc cgagggcgug      960

agccuguccg agaugaugau ccagcgcuac gaggaccacg ccgaccaccu caagcugcug     1020

cgggacauca uccacaacac cgacgccgag aaggccgccg gccuccaggc ggccuacgac     1080

ggguacaucg acggcgugaa ggggaagaag acgcccgucg acgacuucua caaggccauc     1140

aagaagaacc uggacgacag cgaggccgcc caccagaucc uggcgcucau cgacaagaac     1200

gaguucaugc cgaagcagcg cagcaagucc aacggcugga ucccccacca gauccagcag     1260

aaggagcugg accggaucau cgagaaccag aagcaguacu accccuggcu ggccgacgug     1320

aaccccaacg agagccgccg gaagguggcc ccguacaaga ucgacgagcu cgucaccuuc     1380

cgcgugcccu acuacguggg gccccugguc accuccgagg agcagaagaa guacggcagc     1440

aaguucgccu ggauggugcg gaaggaggag aagggcgaga ucacccccug gaacuucgac     1500

cagaaggucg accgcgccgc guccgccacg gaguucauca accggaugac caccauggac     1560

accuaccugc ucaacgagga cgugcugccg gcccacagcc ugaucuacca gaaguacgag     1620

gugcugaacg agcucaacaa ggucaagauc aacggggacg ccaucacgcc cgagcugaag     1680

cagaagaucu accacgaccu guucgagaag cagaccaccg uguccaucaa gaaccuccag     1740

accuaccugg ugagcgaguc cgaggcgagc gagguccccg agaucaaggg ccuggccgac     1800

gagaagaagu uccucuccgg gcugacgacc uaccacgacc ugaugaagau cuucgaccgc     1860

aacgugguga acgaccccga gcgggccgag gacuucgaga agaucauccg cuggagcacc     1920

gucuucgagg acaagcacau cuaccgggag aagcucgacu cccugagcug gcugaccacg     1980

gaccagaagg accgccucgc cgugaagcgc uaccggggcu gggggcgccu guccgagaag     2040

cugcucaucg gccugcggga cgagggccac cgcagcauca uggacuaccu gugggagacc     2100

accuccaacu ucaugcagau ccaggcccag aacgacuuca gcgagaagau cgcggccgag     2160

aacaaggagu uccucgacaa cacccagucc gugcagggga ucaucaacga gcuguacacg     2220

agcccgcaga acaagaaggc cauccggcag acccugcucg ucguggagga caucaagaag     2280

gccuuccacg gcgaggcccc caagcgcauc uucguggagu ucgcgcggga gaacgagagc     2340

acgccccagc gcuccaucca gcggcagcgc caggucgaga acgccuacga gaaggugacc     2400

cagaaccugc ugguggacga caagauccgg gccgagcuca aggacagcau caacgagaag     2460

cgccuguucc acgaccggcu guuccucuac uucaugcagg gcgggaucga cauguacucc     2520

gggaagaaga ucaacaucga caaccugagc gccuacgaca ucgaccacau ccugccccag     2580

uccuucaccg ucgacaacag ccucgacaac cgcgugcuga ccaaccagaa ggacaaccgg     2640

gagaagggca acucccugcc gagcgagcuc uaccgcggcu ccaugaagga gaccugggag     2700

augaugcgga accaggggcu gcugagcaag ggcaaguucc accgccucau gaugaccccc     2760

aacuccauga ccgccuacga ccagcagggg uucaucaacc ggcagcuggu ggagacgcgc     2820

caggucauca agcuggcggu ggagauccuc gccagcgagu ucgaccaggc cgacgagaag     2880

guggagaucg ucuccgugaa ggccggccug acccaccaga ugcggcagga cuucagccug     2940

aucaagaacc gcgacgugaa cgacuaccac cacgcguucg acgccuaccu caccgccuuc     3000

gucggcaccu accugcugaa gcgcuacccc aagcuccggc ccuacuucgu guacggggag     3060

uucaagaagu ucucccagaa gcaggccaac cugacgaacu ucaacuuccu gugggcccuc     3120

aagcacaagg accagguggc ggucgaggac accggcgaga agaucuggaa caagccgcag     3180

gaccuggccu acauccgcca ccuguucgcc uucaagaaga uccucaucag ccgggaggug     3240

uccgagcgcc acggggcccu guucgaccag acccuguaca aggccagcga cgacaaggcg     3300

agcggccagg gguccaagaa gcucaucccc gccaaggaca accggcccac cgagcuguac     3360

ggcggcuaca gcgggcagac guccgccuuc auggccaucg ugaaggucac caagggcaag     3420

aagaccaagu accugguggu gggggucccg accgccgcga ucagccagcu ccagggcaug     3480

gacgugaagu ccagccagua cacggagaag aucuacaaca uccugucccc caagcugcuc     3540

agcaagaacg acgacuucga ggugaucguc cccaaggugc gcuaccggca gcugauccug     3600

gacaacaacc agcuguucac ccuggggucc gacaccuaca aguacaacgc ccgccagcug     3660

gugcucagca acgagguccu ggccgugauc aaccgggaca aguccuucau cgacaagaag     3720

cugagcgagg aggccgagaa ccaggcgcuc aacgacgccu ucgacgagau cguggucgcc     3780

auggacaagc acuucgaccu guacgacaag aacggcuucc gcgagaaggu gaaggccggc     3840

aagcagcggu ucgaggagcu gcccgugucc cucuucagcg aggacaagga gaacgagggg     3900

aucaccaagc gcacgguccu gaagcggauc cugcagggcc uccacgccaa cgcgaccacc     3960

uccgaccuga aggugcugga cgggaaguuc uacaacuga                            3999


<210>  1787
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1787
augaagaacu acaccaucgg ccuggacauc gggguggcca gcgucggcug ggugugcauc       60

gacgagaacu acaagauccu caacuacaac aaccgccacg ccuucggggu gcacgaguuc      120

gaguccgcgg agagcgccgc cggccggcgc cugaagcggg ggaugcgccg gcgcuacaac      180

cggcgcaaga agcggcugca gcuccugcag ucccuguucg acagcuacau caccgacucc      240

ggcuucuuca gcaagacgga cucccagcac uucuggaaga acaacaacga guucgagaac      300

cgcagccuca ccgagguccu guccagccug cggaucucca gccgcaagua ccccaccauc      360

uaccaccucc gguccgaccu gaucgagagc aacaagaaga uggaccugcg ccucguguac      420

cuggcccugc acaaccucgu gaaguaccgg ggccacuucc ugcaggaggg gaacuggucc      480

gaggccgcga gcgccgaggg cauggacgac cagcugcucg agcuggucac ccgcuacgcc      540

gagcuggaga accucucccc gcuggaccug agcgaguccc aguggaaggc cgcggagacg      600

cuccugcuga accggaaccu caccaagacc gaccagagca aggagcugac cgccauguuc      660

gggaaggagu acgagcccuu cugcaagcug guggccggcc ucggggugag ccugcaccag      720

cuguuccccu ccagcgagca ggcccucgcc uacaaggaga cgaagaccaa gguccagcug      780

uccaacgaga acguggagga ggugauggag cugcuccugg aggaggagag cgcgcugcuc      840

gaggccgucc agcccuucua ccagcagguc gugcuguacg agcugcucaa gggcgagacc      900

uacguggcca aggccaaggu guccgccuuc aagcaguacc agaaggacau ggcgagccug      960

aagaaccugc ucgacaagac cuucggcgag aagguguacc gcuccuacuu caucagcgac     1020

aagaacuccc agcgggagua ccagaagucc cacaaggucg aggugcugug caagcuggac     1080

caguucaaca aggaggccaa guucgccgag acguucuaca aggaccucaa gaagcugcug     1140

gaggacaaga gcaagaccag caucgggacc accgagaagg acgagaugcu gcgcaucauc     1200

aaggccaucg acuccaacca guuccuccag aagcagaagg gcauccagaa cgccgcgauc     1260

ccgcaccaga acagccugua cgaggccgag aagauccugc ggaaccagca ggcccacuac     1320

cccuucauca cgaccgagug gaucgagaag gugaagcaga uccucgccuu ccgcaucccc     1380

uacuacaucg ggccccuggu caaggacacc acccaguccc cguucagcug gguggagcgc     1440

aagggcgacg cccccaucac gcccuggaac uucgacgagc agaucgacaa ggcggccucc     1500

gccgaggccu ucaucagccg gaugcgcaag accugcaccu accugaaggg ccaggaggug     1560

cuccccaagu ccagccugac cuacgagcgg uucgaggucc ugaacgagcu caacgggauc     1620

cagcugcgca cgaccggcgc ggagagcgac uuccggcacc gccuguccua cgagaugaag     1680

ugcuggauca ucgacaacgu guucaagcag uacaagaccg ucagcaccaa gcggcuccug     1740

caggagcuga agaagucccc guacgccgac gagcucuacg acgagcacac gggggagauc     1800

aaggaggugu ucggcaccca gaaggagaac gccuucgcca ccagccuguc cggguacauc     1860

agcaugaagu ccauccuggg cgccgugguc gacgacaacc ccgcgaugac cgaggagcuc     1920

aucuacugga ucgccguguu cgaggaccgc gagauccugc accugaagau ccaggagaag     1980

uaccccagca ucacggacgu gcagcggcag aagcucgccc uggucaagcu gcccggcugg     2040

gggcgcuucu cccggcuccu gaucgacggc cugccgcucg acgagcaggg gcagagcgug     2100

cuggaccaca uggagcagua cuccagcgug uucauggagg uccugaagaa caagggcuuc     2160

gggcucgaga agaagaucca gaagaugaac cagcaccagg uggacggcac caagaagauc     2220

cgcuacgagg acaucgagga gcuggccggc ucccccgccc ugaagcgggg gaucuggcgc     2280

agcgugaaga ucgucgagga gcucgugucc aucuucggcg agcccgcgaa caucgugcug     2340

gaggucgccc gggaggacgg ggagaagaag cgcaccaaga gccggaagga ccagugggag     2400

gagcugacca agacgacccu caagaacgac cccgaccuga aguccuucau cggcgagauc     2460

aagagccagg gggaccagcg cuucaacgag cagcgguucu ggcuguacgu gacccagcag     2520

ggcaagugcc ucuacaccgg caaggcccug gacauccaga accugagcau guacgaggug     2580

gaccacaucc ucccgcagaa cuucgucaag gacgacuccc uggacaaccu ggcccucgug     2640

augcccgagg ccaaccagcg caagaaccag guggggcaga acaagaugcc ccuggagauc     2700

aucgaggcga accagcagua cgccaugcgc acccuguggg agcggcucca cgagcugaag     2760

cugaucagcu ccggcaagcu cgggcgccug aagaagccca gcuucgacga ggucgacaag     2820

gacaaguuca ucgcccggca gcugguggag acgcgccaga ucaucaagca cgugcgggac     2880

cuccuggacg agcgcuucuc caagagcgac auccaccugg ucaaggccgg caucgugucc     2940

aaguuccggc gcuucagcga gaucccgaag auccgggacu acaacaacaa gcaccacgcg     3000

auggacgccc ucuucgccgc cgcccugauc caguccaucc ugggcaagua cgggaagaac     3060

uuccucgcgu ucgaccugag caagaaggac cgccagaagc aguggcgguc cgugaagggc     3120

agcaacaagg aguucuuccu guucaagaac uucgggaacc uccgccugca gucccccguc     3180

accggcgagg aggugagcgg gguggaguac augaagcacg ucuacuucga gcugcccugg     3240

cagaccacca agaugacgca gaccggcgac ggcauguucu acaaggaguc caucuucagc     3300

cccaagguga agcaggccaa guacgugucc ccgaagaccg agaaguucgu ccacgacgag     3360

gugaagaacc acagcaucug ccucguggag uucaccuuca ugaagaagga gaaggagguc     3420

caggagacga aguucaucga ccugaaggug aucgagcacc accaguuccu gaaggagccc     3480

gagucccagc ucgccaaguu ccuggccgag aaggagacca acagccccau cauccacgcc     3540

cggaucaucc gcaccauccc gaaguaccag aagaucugga ucgagcacuu ccccuacuac     3600

uucaucagca cccgggagcu gcacaacgcg cgccaguucg agaucuccua cgagcucaug     3660

gagaagguga agcagcugag cgagcggucc agcgucgagg agcugaagau cguguucggg     3720

cuccugaucg accagaugaa cgacaacuac cccaucuaca cgaaguccag cauccaggac     3780

cgcgugcaga aguucgucga cacccagcug uacgacuuca aguccuucga gaucggcuuc     3840

gaggagcuca agaaggccgu ggccgccaac gcccagcgga gcgacaccuu cgggucccgc     3900

aucagcaaga agcccaagcc ggaggaggug gcgaucggcu acgaguccau caccgggcug     3960

aaguaccgga agccccgcag cgucgugggc acgaagcgcu ga                        4002


<210>  1788
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1788
augagccaga acaucgugga cuacugcauc ggccuggacc ucgggaccgg cuccgucggg       60

ugggccgugg uggacaugaa ccaccgccug augaagcgga acggcaagca ccuguggggg      120

agccgccucu ucuccaacgc cgagaccgcg gccaaccggc gcgccagccg guccauccgc      180

cggcgcuaca acaagcggcg cgagcggauc cgccugcugc gggccauccu ccaggacaug      240

guccuggaga acgaccccac guucuucauc cgccuggagc acaccagcuu ccucgacgag      300

gaggacaagg ccaacuaccu gggcgcggac uacaaggaca acuacaaccu guucaucgac      360

gaggacuuca acgacuacac cuacuaccac aaguacccga ccaucuacca ccuccggaag      420

gcccugugcg aguccacgga gaaggccgac ccccgccuga ucuaccucgc ccugcaccac      480

aucgugaagu accggggcaa cuuccuguac gaggggcaga aguucaacau ggacgcgagc      540

aacaucgagg accgccucuc cgacguguuc acccaguucg ccgacuucaa caacaucccc      600

uacgaggacg acgagaagaa gaaccuggag auccuggaga uccucaagaa gccccugagc      660

aagaaggcca aggucgacga ggugauggcc cugaucgccc cggagaagga cuucaagucc      720

gcguacaagg agcucgugac cggcaucgcc gggaacaaga ugaacgucac caagaugauc      780

cugugcgagc ccaucaagca gggcgacagc gagaucaagc ugaaguucuc cgacagcaac      840

uacgacgacc aguucuccga gguggagaac gaccucgggg aguacgugga guucaucgac      900

agccugcaca acaucuacuc cugggucgag cugcagacga ucaugggcgc cacccacacc      960

uacaacgcca gcaucuccga ggccauggug agccgguaca acaagcacca cgaggaccuc     1020

cagcugcuga agaagugcau caaggacaac gugcccaaga aguacuucga cauguuccgc     1080

aacgacagcg agaagcucaa gggcuacuac aacuacauca accaccccuc caaggcgccg     1140

gucgacgagu ucuacaagua cgugaagaag ugcaucgaga agguggacac ccccgaggcc     1200

aagcagaucc ugcacgacau cgagcuggag aacuuccucc ugaagcagaa cagccggacg     1260

aacggguccg uccccuacca gaugcagcug gacgagauga ucaagaucau cgacaaccag     1320

gccaaguacu accccgugcu caaggagaag cgcgagcagc ugcugagcau ccucaccuuc     1380

aagaucccgu acuacuucgg cccccugaac gagaccuccg agcacgccug gaucaagcgc     1440

cuggagggga aggagaacca gcggauccuc cccuggaacu accaggacau cguggacguc     1500

gacgccaccg cggagggcuu caucaagcgc augcggagcu acugcacgua cuuccccgac     1560

gaggaggugc ugccgaagaa cucccugauc gugagcaagu acgaggucua caacgagcuc     1620

aacaagaucc gcguggacga caagcugcug gaggucgaca ucaagaacga caucuacaac     1680

gagcuguuca ugaacaacaa gaccgugacc gagaagaagc ucaagaacug gcuggugaac     1740

aaccagugcu gcaacaagaa cgccgagauc aagggcuucc agaaggagaa ccaguucucc     1800

accagccuga cgcccuggau cgacuucacc aacaucuucg gggagaucaa ccaguccaac     1860

uucgaccuca ucgaggacau caucuacgac cugaccgucu ucgaggacaa gaagaucaug     1920

aagcggcgcc ugaagaagaa guacgcccuc cccgacgaca agaucaagca gauccugaag     1980

cugaaguaca aggacuggag ccggcucucc aagaagcugc uggacggcau cguggccgac     2040

aacaaguucg ggagcuccgu gaccguccuc gacgugcugg agaugagccg ccugaaccuc     2100

auggagauca ucaacgaccg ggaccugggc uacgcccaga ugaucgaggc ggccgccucc     2160

ugccccgagg acgggaaguu cacguacaag gaggugcagc gccuggccgg cagcccggcg     2220

cucaagcggg gcaucuggca gucccugcag aucgucgagg agaucaccaa ggugaugaag     2280

ugccgcccca aguacaucua caucgaguuc gagcggagcg aggagaccaa ggagcgcacc     2340

gagagcaaga ucaagaagcu ggagaacgug uacaaggacc ucgacgagca gacgaagguc     2400

gaguacaaga ccgugcugga ggagcugaag ggguucgaca acaccaagaa gaucuccagc     2460

gacucccucu uccuguacuu cacccagcug ggcaagugca uguacagcgg gaagaagcuc     2520

gacaucgacu cccuggacaa guaccagauc gaccacaucg ugccccagag ccuggucaag     2580

gacgacuccu ucgacaaccg ggugcucgug guccccagcg agaaccagcg caagcuggac     2640

gaccuggugg ugccguccga cauccggguc aagaugaaca gcuucuggaa gcuccuguuc     2700

gaccacgagc ugaucucccc caagaaguuc uacagccuca ucaagacgga guacaccgag     2760

cgcgacgagg agcgguucau caaccgccag cugguggaga cccgccagau caccaagaac     2820

gugacgcaga ucaucgagga ccacuacucc accaccaagg ucgccgccau ccgggccaac     2880

cugagccacg aguuccgcgu gaagaaccac aucuacaaga accgggacau caacgacuac     2940

caccacgccc acgacgcgua caucguggcc cucaucggcg gguucaugcg cgaccgguac     3000

cccaacaugc acgacuccaa ggccgucuac agcgaguaca ugaagauguu ccgcaagaac     3060

aagaacgaca agaagcggug gaaggacggc uucgugauca acuccaugaa cuaccccuac     3120

gagguggacg gcgagcugau cuggaacccg gacaucauca acgagauccg caagugcuuc     3180

uacuacaagg acugcuacug caccaccaag cuggaccaga aguccgggca gauguucaac     3240

cucacggucc ugcccaacga cgcccacagc cccaagggca ccaccgaggc cgugaucccg     3300

gugaacaaga accggaagga cgucaacaag uacgggggcu ucagcgggcu gcaguacgug     3360

aucguggcga ucgagggcaa gaagaagcgc ggcaagaaga ccaagcucgu caagaagauc     3420

agcggggugc cccugcaccu gaaggccgcc ucccucgacg agaagaucaa guacaucgag     3480

gagaaggaga accugacgga cgugaagauc aucaaggaca gcauccccgu caaccagaug     3540

aucgagaugg acggcgggga guaccugcuc accuccccca ucgaguucgu gaacggccgg     3600

cagcuggugc ugaacgagaa gcagugcgcc cucaucgccg acaucuacaa cgcgaucuac     3660

aagcaggacu gcgacaaccu ggacgacguc cugaugaucc agcucuacau cgagcugauc     3720

aacaagauga aggcccugua cccggccuac cagagcaucg ccgagaaguu cgaguccaug     3780

accgaggacu acguggcggu gagcaaggag gagaaggccg acaucaucaa gcagaugcuc     3840

aucaucaugc accgcggccc ccggaacggg aagauccagu acgccgacuu caacgucggc     3900

gaccgcaucg ggcggaagaa caagaugucc cuggaccugg agcgcgugac cuucgugagc     3960

caguccccca cgggcaucua caccaagaag uacaagcucu ga                        4002


<210>  1789
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1789
augggcaagg aguacuaccu ggggcucgac aucggcaccg acagcguggg gugggccguc       60

ugcgacacca acuacaagau ccccaaguuc aagggcaacg ccaugugggg gauccgccug      120

uucgagaagu ccaacagcgc ggaggagcgg cgcggcuucc gguccagccg ccggcgcacg      180

cagcggaagc gcgagcggcu gaagcuccug gagaugcugu ucgacgagga gaucuccaag      240

aucgacccgg ccuucuucau caagcuccgc gagagcaacc uguaccugga ggacaagucc      300

accaagaucc ccuacugcgu guucagcgac gagaacuaca ccgacaagga cuuccaccgg      360

gacuacccca ccaucuacca ccuccgcaac gagcugauca aguccaacaa gccccacgac      420

gugcggcugg ucuaccucgc ccugcaccac aucaucaaga accgcggcca cuuccuguuc      480

gacuacagcu acaagaacga caaguccggg gacuucaugc cgaucuacaa cgaccucaag      540

aacuacuucu acgacaacua cgagaucgag cuggacugca gcgacgugga cgaguucgcc      600

aacacgcuga aggacaaguc caagggcaag accaagaaga acgccgagau cgcggagcug      660

uucgggguga gcaagaagua cgacaagguc cuguacuccg cccugagccu ccuguccggc      720

agcuccguga agcuguucga caucuucgac gacgaggccc ucaaggacgu ggagaagaag      780

uccgucagcu ucuccagcgg guucgacgac aacgccaacg acuuccaguc cuaccugggc      840

gagcgguucg agcugcucga gaagcugaag gcgguguacg acugggccgu gcuggccgac      900

auccucgacg gcaaggagua caucagcgag gccaaggucg ccaucuacga gaagcacaag      960

cgcgaccuga agacccugaa gaaguacauc aagaucuaca ugcccgagaa gaaggcggag     1020

aucuucaaca ucuccagcaa ggacaacaac uacguggccu acuccaagca caucaagacc     1080

agcaacggga cgggcgugcu caucuccacg ugcacccagg aggaguucug cgccuaccug     1140

aagaagaccc uggggaucug caaggacgcc agcuacgagg agauguucaa ggagaucgag     1200

aacuccaccu ucaugcccaa gcaggucacc aaggacaacg gcguggugcc caugcagguc     1260

aacgagaagg agcucguggc cauccuggac aacgcgaagg aguaccugcc guuccuccug     1320

aacaaggaca acgacggcaa gaccguguac gacaagauca aggcccuguu cgaguaccgg     1380

auccccuacu acaucgggcc ccucaacacc cacagcacga aguccuggcu gguccgcagc     1440

aacgagaaga ucuaccccug gaacaucgag gacguggugg acuucgacaa guccgccgag     1500

aaguucaucg agaaccugac cagcaagugc accuaccucc cgcaguacga cgucaucccc     1560

aagcacuccc ugcuguacaa caaguucugc gugcucaacg agcugaacaa ccugaagauc     1620

gacggcgaga agaucagcgu ccagcugaag caggacaucu acaacgaccu cuucaagaag     1680

uacuacaagg ugaccaacaa gaagcugugc gacuaccuga agucccggaa caucgccuuc     1740

gacgagauca gcgggaucga caacgacuuc aagagcuccc ucaagagcua caaggaccug     1800

guggccuaca accugacgga cgagcagaag gaggagaucg ucaaggcgau caccaucuuc     1860

ggcgacgaca agaagcuccu gaagaagcgc cugaagaacg aguucggggg cugccucucc     1920

gacgacgaga ucaagaagau cugcaagcug aaguacaccg gcuggagcaa gcugucccgg     1980

gaguuccuca ccgacgugac ggccguguac aaggagaccg gggagcugau caacaucauc     2040

ugcgcccugu gggagaccaa cuacaaccuc accagccugc uguacuccga cgaguucugc     2100

ugcgacgaca aggagaacaa gacguucaac gacaagauca acgagcucaa cagcuucggc     2160

aacgacaaga cccugaacca gaucgucgag gagcuguacg ugagcccccu cgugaagcgc     2220

cccaucuacc agagccugcu gaucaccaag gagcucgaga agaucaugaa gugcccgccc     2280

aagaagaucu ucgucgaggu ggcccgcggg gccgaggaga agaagcggac cgugucccgc     2340

cggaacaagc ugcuggaccu cuacaagagc ugcaagaacg acuacccccu gcuguucgac     2400

gagcuccaga acaacacgac cgacgacgag cugaaguccg acaagcugua ccucuacuuc     2460

acccaguucg gcaagugcau guacaccggg gagaacaucg agcugggcga ccuguucaac     2520

aagaaccucu acgacaucga ccacaucuac ccccagagca agaucaagga cgacucccug     2580

gacaaccgcg uccuggugaa gaagacgacc aacgcgaaga aggacaacga guacccgcuc     2640

agcucccaga uccgggagaa gaugacgccc cacuggaagg cccugcugga caagggccuc     2700

aucaacaaga agaaguacga gcgccuggug cggaacuacc cccugagcga cgaggagcuc     2760

agccaguuca ucucccgcca gcuggucgag acccggcaga gcaccaaggc cguggccaac     2820

auccuggagc agcucuaccc ccgcccggac accgagaucg uguacgucaa ggcggcccug     2880

gccuccgagu uccggcacca guacgacaug cugaagugcc gcgaggugaa cgaccuccac     2940

cacgccaagg acgccuaccu gaacaucgug gucgggaacg cguacaacgg caaguucacc     3000

cacaacaagg ccaacuucau caaggggcug cagaacaacg acaaggccua caccgugaag     3060

cucgacagca ugcugaagca caacaucgac ggcgccugga ucgccgacaa caacgagucc     3120

cugaacaucg ucaagagcac caugaacaag aacaacaucc gguacacgcg cuacgcguuc     3180

gagaagaagg gcggccucuu caaggugacc auccugaaga aggggaacgg gcaggccucc     3240

aucaagaaga acgacaagcg gaacagcauc gagaaguacg gcggguacaa ccaggugucc     3300

agcaccuacu ucuccugcgu ggagcacggc aaggacaaca accgcaugau cagcauguuc     3360

uccgucaacc uguacgacaa gaacaaguac gaggagaacc ccaucgccua ccucaucgag     3420

aacuacggcc ugaucaaccc caucaucaag gugaagaagg ugaagaucca gagcugcauc     3480

uccuucgaca acaugcugug ccaccucggg ggcaaggaca gcgggggcuc ccagaucagc     3540

uaccggcccg ggguccagcu ggugcugggc uacgacaacg agcgcuacau ccggaacgug     3600

auccgcugcg ccggcgggga ccuccaccuc cagaccucca agaacgagaa ggacaucguc     3660

acggccgaga agaacaucga gcuguucaac cuccugaucg agaagaugaa gaacaacauc     3720

uacggcgugc uguacagcaa ccucggggac aagcuguacu gcagcaagga gaaguucgag     3780

aagcuggaca ucguggagca gugcaacguc aucaagaacg ugcucaccau ccugaagaac     3840

aacgcgucca agggcgaccu caagagcauc gggaucaagg ccaacggcgc cauccgccug     3900

aacaacaaca ucuccgagau caagaagguc aagagcauca agcugaucaa ccaguccgug     3960

accggccugu ucgagcagga gaucgagcuc cugaaccugu ga                        4002


<210>  1790
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1790
augagccaga acaucgugga cuacugcauc ggccuggacc ucgggaccgg cuccgucggg       60

ugggccgugg uggacaugaa ccaccgccug augaagcgga acggcaagca ccuguggggg      120

agccgccucu ucuccaacgc cgagaccgcg gccacgcggc gcagcucccg gagcauccgc      180

cggcgcuaca acaagcggcg cgagcggauc cgccugcugc gggccauccu ccaggacaug      240

guccuggaga aggaccccac cuucuucauc cgccuggagc acaccuccuu ccucgacgag      300

gaggacaagg ccaaguaccu gggcaccgac uacaaggaca acuacaaccu guucaucgac      360

gaggacuuca acgacuacac guacuaccac aaguacccga ccaucuacca ccuccggaag      420

gcccugugcg agagcaccga gaaggcggac ccccgccuga ucuaccucgc ccugcaccac      480

aucgugaagu accggggcaa cuuccuguac gaggggcaga aguucaacau ggacgccucc      540

aacaucgagg acaagcucag cgacguguuc acccaguucg ccgacuucaa caacaucccc      600

uacgaggacg acgagaagaa gaaccuggag auccuggaga uccucaagaa gccccugucc      660

aagaaggcga aggucgacga ggugauggcc cugaucagcc cggagaagga guucaagucc      720

gccuacaagg agcucgugac gggcaucgcc gggaacaaga ugaacgucac caagaugauc      780

cugugcgaga gcaucaagca gggcgacucc gagaucaagc ugaaguucag cgacuccaac      840

uacgacgacc aguucagcga gguggagaac gaccucgggg aguacgugga guucaucgac      900

ucccugcaca acaucuacag cugggucgag cugcagacca ucaugggcgc cacccacacg      960

gacaacgcga gcaucuccga ggccauggug agccgcuaca acaagcacca cgaggaccuc     1020

cagcugcuga agaagugcau caaggacaac gugcccaaga aguacuucga cauguuccgg     1080

aacgacuccg agaaggucaa gggcuacuac aacuacauca accgccccaa caaggccccc     1140

guggacgagu ucuacaaguu cgugaagaag ugcaucgaga aggucgacac cccggaggcc     1200

aagcagaucc uccacgacau cgagcuggag aacuuccugc ucaagcagaa cagccggacc     1260

aacggguccg ugcccuacca gaugcagcug gacgagauga ucaagaucau cgacaaccag     1320

gccaaguacu accccgugcu gaaggagaag cgcgagcagc uccugagcau ccugaccuuc     1380

cgcauccccu acuacuucgg cccgcucaac gagacguccg agcacgcgug gaucaagcgg     1440

cuggagggga aggagaacca gcgcauccug cccuggaacu accaggacac cgucgacgug     1500

gacgccaccg ccgagggcuu caucaagcgg augcgcagcu acugcaccua cuuccccgac     1560

gaggaggugc uccccaagaa cucccugauc gucagcaagu acgaggugua caacgagcug     1620

aacaagaucc gggucgacga caagcuccug gagguggacg ugaagaacga caucuacaac     1680

gagcuguuca ugaagaacaa gacggucacc gagaagaagc ugaagaacug gcucgugaac     1740

aaccagugcu gccgcaagga cgccgagauc aagggcuucc agaaggagaa ccaguucucc     1800

accagccuga ccccguggau cgacuucacg aacaucuucg ggaagaucga ccaguccaac     1860

uucgaccuga ucgagaagau caucuacgac cucaccgugu ucgaggacaa gaagaucaug     1920

aagcggcgcc ugaagaagaa guacgcccug cccgacgaca agaucaagca gauccucaag     1980

cugaaguaca aggacuggag ccggcugucc aagaagcucc uggacggcau cgucgcggac     2040

aaccgcuucg ggagcuccgu gaccgugcug gacguccucg agaugagccg gcugaaccug     2100

auggagauca ucaacgacaa ggagcucggc uacgcccaga ugaucgagga ggccuccagc     2160

ugccccaagg acgggaaguu caccuacgag gagguggcca agcuggccgg cagccccgcg     2220

cugaagcgcg gcaucuggca gucccuccag aucguggagg agaucacgaa ggucaugaag     2280

ugccggccga aguacaucua caucgaguuc gagcgcagcg aggaggccaa ggagcggacc     2340

gaguccaaga ucaagaagcu ggagaacgug uacaaggacc uggacgagca gaccaagaag     2400

gaguacaaga gcgugcucga ggagcugaag ggguucgaca acaccaagaa gaucuccagc     2460

gacucccugu uccucuacuu cacgcagcug ggcaagugca uguacagcgg gaagaagcug     2520

gacaucgacu cccucgacaa guaccagauc gaccacaucg ucccccagag ccuggugaag     2580

gacgacuccu ucgacaaccg cgugcugguc cuccccagcg agaaccagcg gaagcuggac     2640

gaccuggugg ugcccuucga cauccgcgac aagauguacc gguucuggaa gcuccuguuc     2700

gaccacgagc ugaucucccc gaagaaguuc uacagccuca ucaagaccga guacaccgag     2760

cgcgacgagg agcgcuucau caaccggcag cuggucgaga cccgccagau cacgaagaac     2820

gugacccaga ucaucgagga ccacuacucc accaccaagg uggccgccau ccgggcgaac     2880

cugagccacg aguuccgcgu caagaaccac aucuacaaga accgggacau caacgacuac     2940

caccacgccc acgacgccua caucguggcc cucaucggcg gguucaugcg cgaccgguac     3000

cccaacaugc acgacuccaa ggccguguac agcgaguaca ugaagauguu ccgcaagaac     3060

aagaacgacc agaagaagug gaaggacggc uucgucauca acagcaugaa cuaccccuac     3120

gagguggacg gcaagcugau cuggaacccc gaccugauca acgagaucaa gaagugcuuc     3180

uacuacaagg acugcuacug caccacgaag cucgaccaga aguccgggca gauguucaac     3240

cugaccgugc ugccgaacga cgcgcacagc gccaagggca ccaccgaggc cgucaucccc     3300

gugaacaaga accggaagga cgugaacaag uacgggggcu ucuccgggcu ccaguacguc     3360

aucgccgcca ucgagggcac gaagaagaag ggcaagaagc uggugaaggu gcgcaagcug     3420

agcgggaucc cccucuaccu gaagcaggcg gacaucaagg agcagaucga guacgucgag     3480

aaggaggaga agcuguccga cgugaagauc aucaagaaca acaucccgcu caaccagcug     3540

aucgagaucg acggccggca guaccugcuc accagcccca ccgagugcgu gaacgccaug     3600

cagcuggucc ugaacgagga gcagugcaag cucaucgccg acaucuacaa cgccaucuac     3660

aagcaggacu ucgacgggcu ggacaacaug cugaugaucc agcucuaccu gcagcugauc     3720

gacaagcuca agacccugua ccccaucuac augggcaucg uggagaaguu cgagaagcug     3780

acggaggacu ucguguccau cagcaaggag gagaaggcca acgucaucaa gcagaugcuc     3840

aucaucaugc acaagggccc ccagaacggg aacaucaucu acgacgacuu caacgugggc     3900

aagcgcaucg ggcggcugaa cggccgcacc uucuaccugg acaacaucga guucaucucc     3960

cagagcccga ccgggaucua caccaagaag uacaagcucu ga                        4002


<210>  1791
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1791
auguacaccu acuacuucaa ggagagcgag augguguacg acgucggccu ggacaucggg       60

accggcuccg uggggugggu ggcccucgac gagaacggca agcuggcccg cgcgaagggg      120

aagaaccugg ucggcgugcg gcuguucgac acggcccaga ccgccgccga ccgccggggc      180

uuccgcacca cccggcgccg gcugagccgc cggaaguggc gccugcggcu ccuggacgag      240

cuguucuccg ccgagaucaa cgagaucgac agcuccuucu uccagcgccu caaguacagc      300

uacgugcacc ccaaggacga ggagaacaag gcgcacuacu acgggggcua ccuguucccg      360

acggaggagg agaccaagaa guuccaccgg uccuacccca ccaucuacca ccuccgccag      420

gagcucaugg cccagcccaa caagcgguuc gacauccgcg agaucuaccu ggccauccac      480

caccugguca aguaccgggg gcacuuccuc agcucccagg agaagaucac caucggcagc      540

acguacaacc ccgaggaccu ggccaacgcg aucgaggugu acgccgacga gaaggggcug      600

uccugggagc ucaacaaccc ggagcagcug accgagauca ucagcggcga ggccggcuac      660

gggcugaaca aguccaugaa ggccgacgag gcccucaagc uguucgaguu cgacaacaac      720

caggacaagg uggcgaucaa gacccugcuc gccggccuga ccgggaacca gaucgacuuc      780

gccaagcugu ucggcaagga caucagcgac aaggacgagg ccaagcucug gaagcugaag      840

cuggacgacg aggcccucga ggagaagucc cagacgaucc ugagccagcu gaccgacgag      900

gagaucgagc uguuccacgc ggucgugcag gccuacgacg gcuucgugcu gaucgggcug      960

cucaacggcg ccgacuccgu cagcgccgcc auggugcagc uguacgacca gcaccgcgag     1020

gaccggaagc ugcucaaguc ccuggcgcag aaggccgggc ugaagcacaa gcgcuucagc     1080

gagaucuacg agcagcucgc ccuggccacc gacgaggcca ccaucaagaa cggcaucagc     1140

acggcgcggg agcuggugga ggaguccaac cucagcaagg aggucaagga ggacacccug     1200

cgccgccugg acgagaacga guuccucccc aagcagcgga ccaaggccaa cuccgugauc     1260

ccccaccagc ugcaccuggc cgagcugcag aagauccucc agaaccaggg gcaguacuac     1320

cccuuccugc uggacaccuu cgagaaggag gacggccagg acaacaagau cgaggagcuc     1380

cugcgcuucc ggaucccgua cuacgugggc ccccugguca cgaagaagga cguggagcac     1440

gccgggggcg acgcggacaa ccacugggug gagcgcaacg agggguucga gaagucccgg     1500

gucacccccu ggaacuucga caagguguuc aaccgcgaca aggccgcccg ggacuucauc     1560

gagcgccuca ccggcaacga caccuaccug aucggggaga agacgcugcc ccagaacagc     1620

cuccgguacc agcuguucac cgugcugaac gagcucaaca acguccgcgu gaacggcaag     1680

aaguucgaca gcaagaccaa ggccgaccug aucaacgacc uguucaaggc ccggaagacc     1740

gucucccuca gcgcgcugaa ggacuaccug aaggcccagg gcaaggggga cgugacgauc     1800

accggccucg ccgacgaguc caaguucaac agcucccuga gcuccuacaa cgaccugaag     1860

aagaccuucg acgccgagua ccucgagaac gaggacaacc aggagacccu ggagaagauc     1920

aucgagaucc agacgguguu cgaggacagc aagaucgccu cccgcgagcu gagcaagcuc     1980

ccgcuggacg acgaccaggu caagaagcug ucccagaccc acuacaccgg guggggccgg     2040

cucagcgaga agcugcugga cuccaagauc aucgacgagc gcgggcagaa ggugagcauc     2100

cucgacaagc ucaagagcac cucccagaac uucaugagca ucaucaacaa cgacaaguac     2160

ggcgugcagg cguggaucac ggagcagaac accggcucca gcaagcugac cuucgacgag     2220

aaggucaacg agcucaccac cucccccgcc aacaagcggg ggaucaagca gagcuucgcc     2280

gugcugaacg acaucaagaa ggccaugaag gaggagcccc gccgggugua ccuggaguuc     2340

gcccgcgagg accagacguc cguccggagc gugccccgcu acaaccagcu caaggagaag     2400

uaccagucca agagccuguc cgaggaggcg aaggugcuga agaagacccu cgacggcaac     2460

aagaacaaga ugagcgacga ccgcuacuuc cuguacuucc agcagcaggg gaaggacaug     2520

uacaccggcc ggccgaucaa cuucgagcgc cugucccagg acuacgacau cgaccacauc     2580

aucccccagg ccuucaccaa ggacgacagc cucgacaacc ggguccuggu gucccgcccc     2640

gagaacgccc gcaagagcga cuccuucgcc uacacggacg aggugcagaa gcaggacggc     2700

agccugugga ccagccuccu gaaguccggg uucaucaacc ggaagaagua cgagcggcug     2760

accaaggcgg gcaaguaccu cgacgggcag aagaccggcu ucaucgcccg ccagcugguc     2820

gagacgcggc agaucaucaa gaacguggcc agccugaucg agggggagua cgagaacucc     2880

aaggccgugg ccauccgcag cgagaucacc gcggacaugc ggcuccuggu cggcaucaag     2940

aagcaccgcg agaucaacuc cuuccaccac gccuucgacg cccugcucau caccgccgcc     3000

ggccaguaca ugcagaaccg guaccccgac cgcgacagca ccaacgugua caacgaguuc     3060

gaccgguaca cgaacgacua ccugaagaac cugcgccagc ucuccagccg ggacgaggug     3120

cgccggcuga aguccuucgg guucgucgug ggcaccaugc gcaaggggaa cgaggacugg     3180

agcgaggaga acaccuccua ccugcggaag gugaugaugu ucaagaacau ccucaccacg     3240

aagaagaccg agaaggaccg cggcccgcug aacaaggaga ccaucuucag ccccaagucc     3300

gggaagaagc ugaucccccu caacagcaag cgcuccgaca ccgcgcugua cggcggcuac     3360

agcaacgucu acuccgccua caugacgcug gugcgggcca acgggaagaa ccuccugauc     3420

aagaucccca ucagcaucgc caaccagauc gaggugggca accugaagau caacgacuac     3480

aucgucaaca acccggccau caagaaguuc gagaagaucc ucaucuccaa gcugccccug     3540

gggcagcucg ugaacgagga cggcaaccug aucuaccugg cgagcaacga guaccgccac     3600

aacgccaagc agcucuggcu gagcaccacc gacgccgaca agaucgccuc caucagcgag     3660

aacuccagcg acgaggagcu gcucgaggcg uacgacaucc ugaccuccga gaacgugaag     3720

aaccgguucc ccuucuucaa gaaggacauc gacaagcuga gccagguccg cgacgaguuc     3780

cucgacuccg acaagcggau cgccgugauc cagacgaucc ugcgcgggcu gcagaucgac     3840

gccgccuacc aggcccccgu gaagaucauc agcaagaagg ucuccgacug gcacaagcuc     3900

cagcagagcg gcggcaucaa gcuguccgac aacagcgaga ugaucuacca guccgcgacc     3960

gggaucuucg agacccgggu gaagaucagc gaccugcucu ga                        4002


<210>  1792
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1792
augaagaacc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacgaacc aguacgaccu ggugaagcgc aagaugaagg uggccgggaa cuccgacaag      120

aagcagauca agaagaacuu cuggggcguc cggcuguucg acgacgggca gaccgcggug      180

gaccgccgga ugaaccgcac cgcccggcgc cggaucgagc gccggcgcaa ccggaucagc      240

uaccuccagg agaucuucgc cguggagaug gccaacaucg acgccaacuu cuucugccgc      300

cugaacgacu ccuucuacgu cgacagcgag aagcggaacu cccgccaccc guucuucgcg      360

accaucgagg aggagguggc cuaccacaag aacuaccgga cgaucuacca ccuccgcgag      420

gagcucguga acagcuccga gaaggccgac cugcggcugg ucuaccucgc ccuggcgcac      480

aucaucaagu accgcggcaa cuuccugauc gagggcgccc ucgacaccaa gaacaccagc      540

guggacgggg uguacgagca guucauccag accuacaacc aggucuucau guccaacauc      600

gaggagggca cgcuggccaa gguggaggag aacaucgagg uggccaacau ccuggccggg      660

aaguucaccc ggcgcgagaa guucgagcgg auccuccagc uguaccccgg cgagaagucc      720

accgggaugu ucgcgcaguu caucagccug aucgucggca gcaagggcaa cuuccagaag      780

guguucgacc ucaucgagaa gaccgacauc gagugcgcca aggacuccua cgaggaggac      840

cuggaggccc ugcucgccau caucggggac gaguacgccg agcuguucgu ggcggccaag      900

aacacguaca acgccgucgu gcugagcucc aucaucaccg ugaccgccac cgagacgaac      960

gccaagcuca gcgcguccau gaucgagcgc uucgacgccc acgagaagga ccugggcgag     1020

cugaaggccu ucaucaagcu ccaccucccc aagcaguacc aggagaucuu caacaacgcc     1080

gccaucgacg gguacgcggg cuacaucgac ggcaagacca agcaggucga cuucuacaag     1140

uaccugaaga ccauccucga gaacaucgag ggggccgacu acuucaucgc caagaucgag     1200

gaggagaacu uccugcgcaa gcagcggacc uucgacaacg gcgccauccc ccaccagcug     1260

caccucgagg agcuggaggc gaucauccac cagcaggcca aguacuaccc guuccugcgc     1320

gaggacuacg agaagaucaa gagccucgug acguuccgga uccccuacuu cguggggccc     1380

cuggccaagg gccaguccga guucgccugg cugacccgca aggccgacgg ggagauccgg     1440

cccuggaaca ucgaggagaa ggucgacuuc ggcaagagcg cgguggacuu caucgagaag     1500

augaccaaca aggacaccua ccucccgaag gagaacgucc ugcccaagca cagccugugc     1560

uaccagaagu acauggugua caacgagcuc acgaaggugc gcuacaucga cgaccagggc     1620

aagaccaacu acuucuccgg gcaggagaag cagcagaucu ucaacgaccu guucaagcag     1680

aagcggaagg ucaagaagaa ggaccuggag cuguuccugc gcaacaucaa ccacaucgag     1740

agccccacca ucgagggccu ggaggacucc uucaacgcca gcuacgccac cuaccacgac     1800

cuccugaagg uggggaugaa gcaggagauc cuggacaacc cccugaacac ggagaugcuc     1860

gaggacaucg ugaagauccu gaccgucuuc gaggacaagc cgaugaucaa ggagcagcug     1920

cagcaguucu ccgacgugcu cgacggcggg gugcugaaga agcuggagcg gcgccacuac     1980

accggcuggg gccggcucag cgccaagcug cuggucggga uccgcgagaa gcagucccac     2040

cucaccaucc uggacuaccu gaugaacgac gacggccuca accggaaccu gaugcagcug     2100

aucaacgaca gcaaccucuc cuucaagagc aucaucgaga aggagcaggu cuccacgacc     2160

gacaaggacc ugcagagcau cguggccgac cuggcggggu cccccgccau caagaagggc     2220

auccuccaga gccugaagau cgucgacgag cuggugucca ucauggggua cccgccccag     2280

accaucgugg ucgagauggc ccgcgagaac cagaccacgg gcaagggcaa gaacaacagc     2340

aagccccggu acaagucccu cgagaaggcc aucaaggagu ucgggagcaa gauccugaag     2400

gagcacccca ccgacaacca ggagcugaag aacaaccgcc ucuaccugua cuaccugcag     2460

aacggcaagg acauguacac cgggcaggag cucgacaucc acaaccuguc caacuacgac     2520

aucgaccaca ucgugcccca gagcuucauc accgacaaca gcaucgacaa ccuggugcuc     2580

accuccagcg ccggcaaccg ggagaagggc ggggacgugc ccccgcugga gaucgugcgc     2640

aagcggaagg uguucuggga gaagcuguac cagggcaacc ucauguccaa gcgcaaguuc     2700

gacuaccuga cgaaggcgga gcgcgggggc cugaccgacg ccgacaaggc ccgguucauc     2760

caccgccagc ucgucgagac ccggcagauc accaagaacg uggccaacau ccugcaccag     2820

cgcuucaaca acgagacgga caaccacggg aacaccaugg agcagguccg gaucgugacc     2880

cucaagagcg cgcugguguc ccaguuccgc aagcaguucc agcuguacaa ggugcgggag     2940

gucaacgacu accaccacgc ccacgacgcc uaccugaacg gcgugguggc caacacccuc     3000

cugaaggucu acccccagcu ggagcccgag uucguguacg gcgaguacca ccaguucgac     3060

ugguucaagg ccaacaaggc gacggccaag aagcaguucu acaccaacau caugcuguuc     3120

uucgggcaga aggagcgcau caucgacgag aacggcgaga uccuguggga caagaaguac     3180

cuggagacca ucaagaaggu gcucgacuac cggcagauga acaucgucaa gaagaccgag     3240

auccagaagg gggaguucag caaggccacg aucaagccca agggcaacuc cagcaagcug     3300

aucccgcgca aggagaacug ggaccccaug aaguacgggg gccuggacuc ccccaacaug     3360

gccuacgccg ugaucaucga gcacgcgaag ggcaagaaga agcucaucuu cgagaagaag     3420

aucauccgga ucaccaucau ggagcgcaag auguucgaga aggacgagga ggccuuccug     3480

gaggagaagg gguaccggca ccccaaggug cugaccaagc ucccgaagua cacccuguac     3540

gagugcgaga agggccgccg gcgcaugcug gccagcgcca acgaggccca gaaggggaac     3600

cagcucgucc uguccaacca ccuggugagc cuccuguacc acgcgaagaa cugcgaggcc     3660

uccgacggca agagccugaa guacaucgag gcccaccggg agacguucuc cgagcuccug     3720

gcccagguga gcgaguucgc gacccgcuac acccuggccg acgccaaccu cuccaagauc     3780

aacaaccugu ucgagcagaa caaggagggg gacauccagg ccaucgccca gagcuucguc     3840

gaccugaugg cguucaacgc caugggcgcc cccgccagcu ucaaguucuu cgaggccacc     3900

aucgaccgga agcgcuacac gaaccucaag gagcugcugu ccagcaccau caucuaccag     3960

uccaucaccg gccucuacga gagccggaag cgccuggacg acuga                     4005


<210>  1793
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1793
augaagaagc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccgcc       60

cucacggacc aguacgaccu ggucaagcgc aagaugaagg uggcggggaa cuccgagaag      120

aagcagauca agaagaaccu guggggcgug cggcucgucg acgaggggaa gaccgccgcc      180

caccgccggg ugaaccgcac cacccggcgc cggaucgagc gccggcgcaa ccggaucagc      240

uaccugcagg agaucuucac ggccgagaug uucgaggugg acgccaacuu cuucuaccgc      300

cuggaggacu ccuucuacau cgagagcgag aagcggcagu cccgccaccc guucuucgcg      360

accaucgagg aggaggucgc cuaccacgag aacuaccgga ccaucuacca ccuccgcgag      420

aagcugguga acagcuccga caaggccgac cugcggcucg uguaccuggc ccuggcgcac      480

aucaucaagu accgcggcaa cuuccucauc gagggcaagc uggacaccaa gaacacgagc      540

gucgacgagg uguucaagca guucaucaag accuacaacc agguguucgc cuccgacauc      600

gaggagggga gccugacccg gaucgaggag aacaacgagg ucgccaagau cuucuccgag      660

aagcucacca agcgcgagaa gcuggacaag auccugaacc ucuaccccaa cgagaagucc      720

acggaccugu ucgcccaguu caucagccug aucaucggca gcaaggggaa cuucaagaag      780

uucuucaacc ucaccgagaa gaccgacauc gagugcgcca aggacuccua cgaggaggac      840

cuggaggugc ugcucgcgcg ggugggcgac gaguacgccg agaucuucgu cgccgccaag      900

aacgccuaca acgcgguggu gcugagcucc aucaucaccg ucagcaacac ggagaccaag      960

gccaagcugu ccgccagcau gaucgagcgc uucgacaagc acgacaagga ccucaagcgc     1020

augaaggccu ucuucaaggu gcggcugccc gagaacuuca acgagguguu caacgacguc     1080

gagaaggacg gguacgccgg cuacaucgag ggcaagacca agcaggaggc guucuacaag     1140

uacaugaaga agaugcugga gcacguggag ggggccgacu acuucaucaa ccagaucgag     1200

gaggagaacu uccuccgcaa gcagcggacc uucgacaacg gcgccauccc ccaccagcug     1260

caccuggagg agcucgaggc cauccugcac cagcaggcca aguacuaccc guuccugaag     1320

guggacuacg agaagaucaa gagccucguc acguuccgca uccccuacuu cguggggccc     1380

cuggcgaacg gccaguccga guucagcugg cugacccgga aggccgacgg cgagauccgc     1440

cccuggaaca ucgaggagaa ggucgacuuc gggaaguccg ccaucgacuu caucgagaag     1500

augaccaaca aggacaccua ccucccgaag gagaacgugc ugcccaagca cagcaugugc     1560

uaccagaagu acauggugua caacgagcug acgaagaucc gguacaccga cgaccagggc     1620

aagacccacu acuucuccgg gcaggagaag cagcagaucu ucaacgaccu cuucaagcag     1680

aagcgcaagg ucaagaagaa ggaccuggag cuguuccucu acaacaugaa ccacguggag     1740

agccccaccg uggagggcgu cgaggacgcc uucaacucca gcuucacgac cuaccacgac     1800

cugcagaagg ugggggugcc ccaggagauc cuggacgacc cgcucaacac cgagaugcug     1860

gaggagauca ucaagauccu gaccgucuuc gaggacaagc ggaugaucaa cgagcgccug     1920

caggaguucu ccaacgugcu cgacgaggcg gugcugaaga agcuggagcg gcgccacuac     1980

acgggcuggg gccggcucag cgccaagcug cugaucggga uccgcgacaa ggagucccac     2040

cucaccaucc uggacuaccu gaugaacgac gacaagcaca accggaaccu caugcagcug     2100

aucaacgaca gcaaccuguc cuucaagagc aucaucgaga aggagcaggu cuccaccgcc     2160

gacaaggaca uccagagcau cguggccgac cucgccggcu cccccgcgau caagaagggg     2220

auccugcaga gccugaagau cguggacgag cucgucggca ucauggggua cccgccccag     2280

accaucgugg uggagauggc ccgcgagaac cagacgaccg gcaagggcaa gaacaacucc     2340

aagccccggu ucaccagccu ggagaaggcc aucaaggagc uggggagcca gauccucaag     2400

gagcacccca ccgacaacca gggccugaag aacgaccgcc uguaccucua cuaccugcag     2460

aacgggaagg acauguacac cggccaggag cuggacaucc acaaccucuc caacuacgac     2520

aucgaccacg ucgugcccca gagcuucauc acggacaacu ccaucgacaa ccgggugcug     2580

gccagcuccg ccgcgaaccg cgagaagggg gacaacgucc ccagccugga gguggugcgc     2640

aagcggaagg ucuacuggga gaagcucuac caggccaagc ugauguccaa gcgcaaguuc     2700

gacuaccuga ccaaggccga gcggggcggc cucaccgagg ccgacaaggc ccgcuucauc     2760

caccggcagc ugguggagac ccgccagauc acgaagaacg uggcgaacau ccugcaccag     2820

cgguucaacu gcaagaagga cgagagcggg aacgucaucg agcagguccg caucgugacc     2880

cucaaggccg cccugguguc ccaguuccgg aagcaguucc agcuguacaa ggugcgcgag     2940

gugaacgacu accaccacgc ccacgacgcg uaccucaacu gcgucguggc caacacccug     3000

cugaaggugu acccgcagcu cgagcccgag uucgucuacg gcgacuacca ccaguucgac     3060

ugguucaagg ccaacaaggc caccgccaag aagcaguucu acacgaacau caugcuguuc     3120

uucgcgaaga aggaccggau caucgacgag aacggggaga uccuguggga caagaaguac     3180

cucgacacca ucaagaaggu gcugaacuac cgccagauga acaucgugaa gaagaccgag     3240

auccagaagg gcgaguucag caacgccacc gccaacccca agggcaacuc cagcaagcug     3300

aucccccgga aggccgacug ggacccgauc aaguacgggg gcuucgacgg guccaacaug     3360

gccuacgcga ucgucaucga gcacgagaag cgcaagaaga agacggugau caagaaggag     3420

cucauccaga ucaacaucau ggagcggacc gccuucgaga aggaccagaa ggaguuccug     3480

gagggcaagg gguaccgcaa ccccaaggug aucaccaaga uccccaagua cacccuguac     3540

gagugcgaga acggccggcg ccggaugcuc ggcagcgcca acgaggccca gaaggggaac     3600

cagauggucc ugcccaacca ccugaugacg cuccuguacc acgccaagaa cugcgaggcg     3660

uccgacggca agagccuggc cuacaucgag ucccaccgcg agauguucgc cgagcuccug     3720

gacagcauca gcgaguucgc cucccgguac acccuggcgg acgccaaccu cgagaagauc     3780

aacaccaucu ucgagcagaa caagagcggg gacgugaagg ugaucgccca guccuucguc     3840

aaccugcugg aguucaacgc caugggcgcc ccggcgagcu ucaaguacuu cgagaccaac     3900

aucgagcgca agcgcuacaa caaccucaag gagcugcuga acgccacgau caucuaccag     3960

uccaucaccg ggcucuacga ggcccggaag cgccuggacg acuga                     4005


<210>  1794
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1794
augaagaacc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacggacc aguacgaccu ggugaagcgc aagaugaagg uggccgggaa cuccgacaag      120

aagcagauca agaagaacuu cuggggcguc cggcuguucg acgacgggca gaccgcggug      180

gaccgccgga ugaaccgcac cgcccggcgc cggaucgagc gccggcgcaa ccggaucagc      240

uaccuccagg agaucuucgc cguggagaug gccaacaucg acgccaacuu cuucugccgc      300

cugaacgacu ccuucuacgu cgacagcgag aagcggaacu cccgccaccc guucuucgcg      360

accaucgagg aggagguggc cuaccacaag aacuaccgga cgaucuacca ccuccgcgag      420

gagcugguga acagcuccga gaaggccgac cugcggcugg ucuaccucgc ccuggcgcac      480

aucaucaagu accgcggcaa cuuccugauc gagggcgccc ucgacaccaa gaacaccagc      540

guggacgagg uguacaagca guucauccag accuacaacc aggucuucau guccaacauc      600

gaggaggggg cccuggccaa gguggaggag aacacggagg uggccagcau ccuggcgggc      660

aaguucaccc ggcgcgagaa guucgagcgg auccuccgcc uguaccccgg ggagaagucc      720

accggcaugu ucgcccaguu caucagccug aucgucggga acaagggcaa cuuccagaag      780

guguucaacc ucguggagaa gaccgacauc gagugcgcca aggacuccua cgaggaggac      840

cuggaggccc ugcucgccau caucggcgac gaguacgcgg agcuguucgu cgccgccaag      900

aacacguaca acgccguggu gcugagcucc aucaucaccg ucaccgccac cgagacgaac      960

gcgaagcuca gcgccuccau gaucgagcgc uucgacgccc acgagaagga gcugggggag     1020

cugaaggccu ucaucaagcu ccaccucccc aagcaguacc aggagaucuu caacaacgcc     1080

gagaucgacg gcuacgcggg guacaucgac ggcaagacca agcaggucga cuucuacaag     1140

uaccugaaga ccacccugga gaacguggag ggcgccgacu acuucaucac gaagaucgag     1200

gaggagaacu uccugcggaa gcagcgcacc uucgacaacg gggugauccc ccaccagcug     1260

caccucgagg agcuggaggc cauccugcac cagcaggcca aguacuaccc guuccuccgg     1320

gagggcuacg acaagaucaa gagccuggug accuuccgca uccccuacuu cgucgggccc     1380

cuggcgaacg gccaguccga guucgccugg cucacccgga aggacgacgg ggagauccgc     1440

cccuggaaca ucgaggagaa gguggacuuc ggcaagagcg ccguggacuu caucgagaag     1500

augacgaaca aggacaccua ccugccgaag gagaacgucc ugcccaagca cagccucugc     1560

uaccagaagu acauggugua caacgagcug accaagaucc gguacaucga cgaccagggc     1620

aagaccaacu acuucuccgg gcgcgagaag cagcagaucu ucaacgaccu guucaagcag     1680

aagcggaagg ugaagaagaa ggaccucgag cuguuccugc gcaacaucaa ccacaucgag     1740

agccccacga ucgagggccu cgaggacucc uucaacgcca gcuacgccac cuaccacgac     1800

cugcugaagg ucgggcugaa gcaggagauc cucgacaacc cccugaacac cgagauccug     1860

gaggacaucg ugaagauccu caccguguuc gaggacaagc ggaugaucaa ggagcagcug     1920

gagcaguucu ccgacguccu ggacggcgug gugcucaaga agcuggagcg ccggcacuac     1980

acgggguggg gccgccugag cgcgaagcuc cuggucggca uccgggacaa gcagucccac     2040

cugaccaucc ucgacuaccu gaugaacgac gacgggcuga accgcaaccu caugcagcug     2100

aucaacgaca gcaaccuguc cuucaagagc aucaucgaga aggagcaggu cuccaccacc     2160

gacaaggacc uccagagcau cguggccgac cuggccggcu ccccggccau caagaagggg     2220

auccugcaga gccucaagau cguggacgag cuggugucca ucaugggcua cccgccccag     2280

acgaucgugg ucgagauggc ccgggagaac cagaccaccg ggaagggcaa gaacaacagc     2340

aagccccgcu acaagucccu ggagaaggcg aucaaggacu ucggcagcca gauccucaag     2400

gagcacccca ccgacaacca ggagcugaag aacaaccggc uguaccucua cuaccugcag     2460

aacgggaagg acaucuacac cggccaggag cuggacaucc acaaccucuc caacuacgac     2520

aucgaccaca ucgugcccca gagcuucauc acggacaaca gcaucgacaa ccuggugcug     2580

accuccagcg ccgggaaccg cgagaagggc ggcgacgucc cgccccucga gaucgugcgc     2640

aagcggaagg uguucuggga gaagcuguac caggggaacc ugauguccaa gcgcaaguuc     2700

gacuaccuca ccaaggccga gcggggcggg cugaccgagg ccgacaaggc ccgcuucauc     2760

caccggcagc uggucgagac gcgccagauc accaagaacg uggcgaacau ccuccaccag     2820

cgguucaaca aggagaccga caaccacggc aacaccaugg agcagguccg caucgugacg     2880

cucaagagcg cccugguguc ccaguuccgg aagcaguucc agcuguacaa ggugcgcgag     2940

gucaacgacu accaccacgc ccacgacgcc uaccugaacg gggugguggc gaacacccug     3000

cucaaggucu acccccagcu ggagcccgag uucguguacg gcgaguacca ccaguucgac     3060

ugguucaagg ccaacaaggc caccgccaag aagcaguucu acaccaacau caugcuguuc     3120

uucgcccaga aggagcggau caucgacgag aacggcgaga uccucuggga caagaaguac     3180

cuggagacga ucaagaaggu gcuggacuac cgccagauca acaucgucaa gaagaccgag     3240

auccagaagg gggaguucag caaggcgacc aucaagccca agggcaacuc cagcaagcuc     3300

aucccccgga aggagaacug ggacccggug aaguacgggg gccuggacuc ccccaacaug     3360

gccuacgccg ugaucaucga gcacgccaag gggaagaaga agaucgucau cgagaagaag     3420

cugauccaga ucaacaucau ggagcgcaag auguucgaga aggacgagga ggccuuccuc     3480

gaggagaagg gcuaccacca gcccaaggug cugaccaagc ugcccaagua cacgcucuac     3540

gagugcgaga agggccggcg ccggaugcug agcuccgcga acgaggccca gaaggggaac     3600

cagcuggugc ucagcaacca ccuggucucc cugcucuacc acgccaagaa cugcgaggcc     3660

agcgacggca agucccugaa guacaucgag gcccaccgcg agaccuucag cgagcugcuc     3720

gcccaggugu ccgaguucgc gaccaaguac acccuggccg acgccaaccu gagcaagauc     3780

aacaaccucu ucgagcagaa caaggagggg gacaucaagg cgaucgccca gagcuucgug     3840

gaccugaugg ccuucaacgc caugggcgcc ccggcguccu ucaaguucuu cgaggccacg     3900

aucgaccgga agcgcuacac caaccugaag gagcuccuga gcuccaccau caucuaccag     3960

agcaucaccg ggcuguacga gucccggaag cgccucgacg acuga                     4005


<210>  1795
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1795
augggccgca agccguacau ccugagccuc gacaucggga ccggcuccgu gggguacgcc       60

ugcauggaca agggcuucaa cguccugaag uaccacgaca aggacgcccu ggggguguac      120

cucuucgacg gcgcgcugac cgcccaggag cggcgccagu uccggacgag ccgccggcgc      180

aagaaccggc gcaucaagcg gcugggccuc cugcaggagc ugcucgcccc gcuggugcag      240

aaccccaacu ucuaccaguu ccagcgccag uucgccugga agaacgacaa cauggacuuc      300

aagaacaagu cccugagcga gguccucucc uuccuggggu acgagagcaa gaaguacccc      360

accaucuacc accuccagga ggcccuccug cugaaggacg agaaguucga ccccgagcuc      420

aucuacaugg cgcuguacca ccuggugaag uaccggggcc acuuccucuu cgaccaccug      480

aagaucgaga accugaccaa caacgacaac augcacgacu ucguggagcu caucgagacc      540

uacgagaacc ugaacaacau caagcugaac cucgacuacg agaagacgaa ggucaucuac      600

gagauccuga aggacaacga gaugaccaag aacgaccgcg ccaagcgggu gaagaacaug      660

cagaagaagc uggagcaguu cuccaucaug cuccuggggc ugaaguucaa cgagggcaag      720

cuguucaacc acgccgacaa cgccgaggag cugaaggggg cgaaccagag ccacaccuuc      780

gccgacaacu acgaggagaa ccugaccccg uuccucacgg uggagcaguc cgaguucauc      840

gagcgcgcca acaagaucua ccugagccug acccuccagg acauccugaa gggcaagaag      900

uccauggcca ugagcaaggu cgccgcguac gacaaguucc ggaacgagcu gaagcagguc      960

aaggacaucg uguacaaggc cgacuccacc cgcacccagu ucaagaagau cuucgugagc     1020

uccaagaagu cccucaagca guacgacgcc acgcccaacg accagaccuu cagcagccug     1080

ugccuguucg accaguaccu cauccggccc aagaagcagu acucccugcu gaucaaggag     1140

cucaagaaga ucauccccca ggacagcgag cuguacuucg aggccgagaa cgacacccug     1200

cugaaggugc ucaacaccac ggacaacgcc agcaucccga ugcagaucaa ccuguacgag     1260

gcggagacca uccugcgcaa ccagcagaag uaccacgccg agaucaccga cgagaugauc     1320

gagaaggugc ucucccugau ccaguuccgg auccccuacu acgucggccc ccuggugaac     1380

gaccacaccg ccagcaaguu cggguggaug gagcgcaagu ccaacgagag caucaagccc     1440

uggaacuucg acgagguggu cgaccggucc aagagcgcca cgcaguucau ccgccgcaug     1500

accaacaagu gcuccuaccu caucaacgag gacgugcugc cgaagaacag ccugcucuac     1560

caggagaugg aggugcugaa cgagcugaac gccacccaga uccggcucca gaccgacccc     1620

aagaaccgca aguaccggau gaugccccag aucaagcugu ucgcggucga gcacaucuuc     1680

aagaaguaca agacgguguc ccacagcaag uuccuggaga ucaugcucaa cuccaaccac     1740

cgcgagaacu ucaugaacca cggcgagaag cugagcaucu ucgggaccca ggacgacaag     1800

aaguucgccu ccaagcugag cuccuaccag gacaugacca agaucuucgg cgacaucgag     1860

ggcaagcggg cccagaucga ggagaucauc caguggauca ccaucuucga ggacaagaag     1920

auccucgugc agaagcugaa ggagugcuac cccgagcuga cgagcaagca gaucaaccag     1980

cucaagaagc ugaacuacuc cggguggggc cgccugagcg agaagcuccu gacccacgcc     2040

uaccaggggc acuccaucau cgagcugcuc cggcacagcg acgagaacuu cauggagauc     2100

cugaccaacg acgucuacgg cuuccagaac uucaucaagg aggagaacca ggugcagagc     2160

aacaagaucc agcaccagga caucgccaac cugaccacgu ccccggcgcu caagaagggg     2220

aucuggagca ccaucaagcu gguccgcgag cugaccucca ucuucggcga gcccgagaag     2280

aucaucaugg aguucgccac cgaggaccag cagaagggca aggagcagaa gucccggaag     2340

cagcucuggg acgacaacau caagaagaac aagcucaaga gcguggacga guacaaguac     2400

aucaucgacg uggccaacaa gcugaacaac gagcagcugc agcaggagaa gcuguggcug     2460

uaccucagcc agaacgggaa gugcauguac uccggccaga gcaucgaccu ggacgcccug     2520

cucuccccca acgcgacgaa gcacuacgag gucgaccaca ucuucccccg cagcuucauc     2580

aaggacgacu ccaucgacaa caaggugcug gugaucaaga agaugaacca gaccaagggg     2640

gaccaggucc cgcugcaguu cauccagcag cccuacgagc ggaucgccua cuggaagucc     2700

cucaacaagg ccggccugau cagcgacagc aagcugcaca agcucaugaa gcccgaguuc     2760

accgccaugg acaaggaggg guucauccag cgccagcugg uggagacccg gcagaucucc     2820

gugcacgucc gcgacuuccu gaaggaggag uaccccaaca ccaaggugau cccgaugaag     2880

gccaagaugg ugagcgaguu ccggaagaag uucgacaucc ccaagauccg ccagaugaac     2940

gacgcgcacc acgccaucga cgccuaccuc aacggcgucg uguaccacgg cgcccagcug     3000

gccuacccca acguggaccu guucgacuuc aacuucaagu gggagaaggu ccgggagaag     3060

uggaaggcgc ucggggaguu caacacgaag cagaaguccc gcgagcuguu cuucuucaag     3120

aagcuggaga agauggaggu gagccagggc gagcggcuca ucagcaagau caagcuggac     3180

augaaccacu ucaagaucaa cuacucccgc aagcuggcca acauccccca gcaguucuac     3240

aaccagaccg ccgugagccc gaagaccgcc gagcucaagu acgaguccaa caagagcaac     3300

gaggucgugu acaaggggcu gacccccuac cagacguacg uggucgccau caaguccgug     3360

aacaagaagg gcaaggagaa gauggaguac cagaugaucg accacuacgu guucgacuuc     3420

uacaaguucc agaacgggaa cgagaaggag cuggcgcucu accuggccca gcgcgagaac     3480

aaggacgagg uccuggacgc ccagaucgug uacagccuca acaagggcga ccugcuguac     3540

aucaacaacc accccugcua cuucgugucc cggaaggagg ucaucaacgc caagcaguuc     3600

gagcucaccg uggagcagca gcugagccug uacaacguga ugaacaacaa ggagaccaac     3660

gucgagaagc uccugaucga guacgacuuc aucgcggaga aggugaucaa cgaguaccac     3720

cacuaccuga acuccaagcu caaggagaag cgcgugcgga ccuucuucag cgaguccaac     3780

cagacgcacg aggacuucau caaggcccug gacgagcugu ucaaggucgu gaccgccagc     3840

gccacccgcu ccgacaagau cggcagccgg aagaacucca ugacccaccg cgccuuccuc     3900

gggaagggca aggacgugaa gaucgcguac acgagcaucu ccgggcugaa gaccaccaag     3960

cccaagagcc uguucaagcu cgccgagucc cggaacgagc uguga                     4005


<210>  1796
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1796
augaagaacc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacggacc aguacgaccu ggugaagcgc aagaugaagg uggccgggaa cuccgacaag      120

aagcagauca agaagaacuu cuggggcguc cggcuguucg acgaggggga gaccgcggcc      180

gaccgccgga ugaaccgcac cgcccggcgc cggaucgagc gccggcgcaa ccggaucagc      240

uaccuccagg agaucuucgc ccuggagaug gccaacaucg acgcgaacuu cuucugccgc      300

cugaacgacu ccuucuacgu ggacagcgag aagcggaacu cccgccaccc guucuucgcc      360

accaucgagg aggagguggc cuaccacaag aacuaccgga cgaucuacca ccuccgcgag      420

gagcugguca acagcuccga gaaggccgac cugcggcucg uguaccuggc gcuggcccac      480

aucaucaagu accgcggcaa cuuccucauc gagggcgccc uggacaccaa gaacaccagc      540

guggacgggg ucuacaagca guucauccag accuacaacc agguguucau cuccaacauc      600

gaggagggca cgcuggccaa gauggaggag aacaccaccg uggccgacau ccucgcgggg      660

aaguucaccc ggaaggagaa gcuggagcgc auccugcagc ucuaccccgg cgagaagucc      720

acggggaugu ucgcccaguu caucagccug aucgucggca gcaagggcaa cuuccagaag      780

guguucgacc ugguggagaa gaccgacauc gagugcgcca aggacuccua cgaggaggac      840

cucgaggccc ugcuggccau caucggggac gaguacgcgg agcuguucgu cgccgccaag      900

aacaccuaca acgccguggu gcucagcucc aucaucaccg ucacggacac cgagaccaac      960

gccaagcuga gcgcguccau gaucgagcgg uucgacgccc acgagaagga ccuccgcgag     1020

cugaaggccu ucaucaagcu gcaccucccc aagcaguacg aggagaucuu cagcaacgug     1080

gccaucgacg gcuacgccgg guacaucgac ggcaagacca agcaggucga cuucuacaag     1140

uaccugaaga cgcugcucga gaacaucgag ggcgcggacu acuucaucgc caagaucgag     1200

gaggagaacu uccugcgcaa gcagcggacc uucgacaacg gggccauccc ccaccagcug     1260

caccucgagg agcuggaggc cauccugcac cagcaggcga aguacuaccc guucuucaag     1320

gaggccuacg acaagaucaa gucccucgug accuuccgca uccccuacuu cgugggcccc     1380

cuggccaacg ggcagagcga cuucgccugg cugacccgga aggccgacgg cgagauccgc     1440

cccuggaaca ucgaggagaa gguggacuuc ggcaagagcg cggucgacuu caucgagaag     1500

augacgaaca aggacaccua ccucccgaag gagaacgugc ugcccaagca cucccuguac     1560

uaccagaagu acauggucua caacgagcuc accaaggugc gguacaucga cgaccagggg     1620

aagaccaacu acuucagcgg ccaggagaag cagcagaucu ucaacgacua cuucaagaag     1680

aagcgcaagg uguccaagaa ggaccuggag caguuccugc ggaacaugag ccacaucgag     1740

ucccccacga ucgaggggcu cgaggacagc uucaacucca gcuacgccac cuaccacgac     1800

cugcugaagg ucggcaucaa gcaggaggug cuggagaacc cccucaacac cgagaugcug     1860

gaggacaucg ugaagauccu gaccgucuuc gaggacaagc gcaugaucaa ggagcagcuc     1920

cagcaguucu ccgacgugcu ggacggggcc gugcugaaga agcucgagcg gcgccacuac     1980

acgggcuggg ggcggcugag cgccaagcug cucgucggca uccgcgacaa gcagucccac     2040

cugaccaucc uggacuaccu caugaacgac gacggccuga accggaaccu gaugcagcuc     2100

aucaacgaca gcaaccuguc cuucaagagc aucaucgaga aggagcaggu cuccaccacc     2160

gacaaggacc ugcagagcau cguggccgac cucgcggggu ccccggccau caagaagggc     2220

auccugcaga gccugaagau cguggacgag cucgugucca ucauggggua cccgccccag     2280

acgaucgugg ucgagauggc ccgcgagaac cagaccaccg ugaagggcaa gaacaacagc     2340

cggccccgcu acaagagccu ggagaaggcc aucaaggagu ucggguccca gauccugaag     2400

gagcacccca ccgacaacca ggagcuccgg aacaaccgcc uguaccugua cuaccuccag     2460

aacggcaagg acauguacac cggccaggag cuggacaucc acaaccugag caacuacgac     2520

aucgaccaca ucgugcccca guccuucauc acggacaaca gcaucgacaa ccucguccug     2580

accuccagcg ccgggaaccg ggagaagggc gacgacgugc cgccccugga gaucgugcgc     2640

aagcgcaagg ucuucuggga gaagcuguuc caggggaacc ucauguccaa gcggaaguuc     2700

gacuaccuga ccaaggcgga gcgcggcggc cucaccgagg ccgacaaggc cacguucauc     2760

caccggcagc ugguggagac ccgccagauc accaagaacg uggccaacau ccugcaccag     2820

cgguucaaca acgagaccga caaccacggg aacaacaugg agcagguccg caucgugaug     2880

cucaagagcg cgcugguguc ccaguuccgg aagcaguucc agcuguacaa gguccgcgag     2940

gugaacgacu accaccacgc ccacgacgcc uaccucaacg gcguggucgc caacacgcug     3000

cugaaggugu acccccagcu cgagcccgag uucguguacg gggaguacca ccaguucgac     3060

ugguucaagg ccaacaaggc gaccgccaag aagcaguucu acaccaacau caugcuguuc     3120

uucgcccaga aggagcggau caucgacgag aacggcgaga uccuguggga caagaaguac     3180

cucgagacca ucaagaaggu ccuggacuac cgccagauga acaucgugaa gaagacggag     3240

auccagaagg gggaguucag caaggccacc aucaagccca agggcaacuc cagcaagcug     3300

aucccccgga aggagaacug ggacccgaug aaguacggcg ggcucgacuc ccccaacaug     3360

gccuacgcgg ugaucaucga gcacgccaag ggcaagaaga aggucguguu cgagaagaag     3420

aucauccgca ucaccaucau ggagcggaag gccuucgaga aggacgagaa guccuuccug     3480

gagaagcagg gguaccgcca gcccaaggug cugaccaagc uccccaagua cacgcuguac     3540

gagugcgaga acggccggcg ccggaugcug gccagcgcca acgaggcgca gaaggggaac     3600

cagcaggucc ucaagggcca gcugaucacc cugcuccacc acgccaagaa cugcgaggcc     3660

agcgacggca agucccugga cuacaucgag agcaaccgcg agauguucgg ggagcugcuc     3720

gcccacguga gcgaguucgc gaagcgguac acccuggccg acgccaaccu guccaagauc     3780

aaccagcugu ucgagcagaa caaggacaac gacaucaagg ugaucgccca gagcuucguc     3840

aaccucaugg ccuucaacgc gaugggcgcc ccggccuccu ucaaguucuu cgaggccacc     3900

aucgagcgca agcgguacac gaaccugaag gagcuccuga gcgccaccau caucuaccag     3960

uccaucaccg ggcuguacga ggcgcgcaag cgccucgacg gcuga                     4005


<210>  1797
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1797
augaagaacc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacgaacc aguacgaccu ggugaagcgc aagaugaagg uggccgggaa cuccgacaag      120

aagcagauca agaagaacuu cuggggcguc cggcuguucg acgacgggca gaccgcggug      180

gaccgccgga ugaaccgcac cgcccggcgc cggaucgagc gccggcgcaa ccggaucagc      240

uaccuccagg agaucuucgc cguggagaug gccaacaucg acgccaacuu cuucugccgc      300

cugaacgacu ccuucuacgu cgacagcgag aagcggaacu cccgccaccc guucuucgcg      360

accaucgagg aggagguggc cuaccacgac aacuaccgga cgaucuacca ccuccgcgag      420

gagcugguga acagcuccga gaaggccgac cugcggcugg ucuaccucgc ccuggcgcac      480

aucaucaagu accgcggcaa cuuccugauc gagggcgccc ucgacaccaa gaacaccagc      540

guggacgagg uguacaagca guucauccag accuacaacc aggucuucau guccaacauc      600

gaggaggggg cccuggccaa gguggaggag aaccuggagg uggccagcau ccucgcgggc      660

aaguucacgc ggcgcgagaa guucgagcgg auccugcgcc uguaccccgg ggagaagucc      720

accggcaugu ucgcccaguu caucagccuc aucgucggga acaagggcaa cuuccagaag      780

guguucaacc ugguggagaa gaccgacauc gagugcgcca aggacuccua cgaggaggac      840

cuggaggccc uccuggccau caucggcgac gaguacgcgg agcuguucgu cgccgccaag      900

aacaccuaca acgccguggu gcucagcucc aucaucacgg ucaccgccac cgagaccaac      960

gcgaagcuga gcgccuccau gaucgagcgc uucgacgccc acgagaagga ccugggggag     1020

cucaaggccu ucaucaagcu gcaccucccc aagcaguacc aggagaucuu caacaacgcc     1080

gcgaucgacg gcuacgccgg guacaucgac ggcaagacga agcaggucga cuucuacaag     1140

uaccugaaga ccacccugga gaacguggag ggcgccgacu acuucaucac caagaucgag     1200

gaggagaacu uccugcggaa gcagcgcacg uucgacaacg gggugauccc ccaccagcuc     1260

caccuggagg agcuggaggc cauccuccac cagcaggcga aguacuaccc guuccugcgg     1320

gaggacuacg agaagaucaa gagccuggug accuuccgca uccccuacuu cgucggcccc     1380

cucgccaagg ggcaguccga guucgccugg cugacccgga aggccgacgg cgagauccgc     1440

cccuggaaca ucgaggagaa gguggacuuc ggcaagagcg ccguggacuu caucgagaag     1500

augaccaaca aggacacgua ccugccgaag gagaacgucc uccccaagca cagccugugc     1560

uaccagaagu acauggugua caacgagcug accaaggugc gguacaucga cgaccagggg     1620

aagaccaacu acuucuccgg ccaggagaag cagcagaucu ucaacgaccu cuucaagcag     1680

aagcgcaagg ucaagaagaa ggaccuggag cuguuccucc ggaacaucaa ccacaucgag     1740

agccccacca ucgaggggcu ggaggacucc uucaacgcga gcuacgccac guaccacgac     1800

cugcugaagg ugggcaugaa gcaggagucc cucgacagcc cccugaacgc cgagaugcug     1860

gaggacaucg ugaagauccu caccgucuuc gaggacaagc gcaugaucaa ggagcagcug     1920

cagcaguucu ccgacgugcu ggacggggug guccucaaga agcuggagcg gcgccacuac     1980

accggcuggg ggcggcugag cgccaagcuc cuggugggca uccgcgacaa gcagucccac     2040

cugaccaucc ucgacuaccu gaugaacgac gacggccuga accggaaccu caugcagcug     2100

aucaacgaca gcaaccuguc cuucaagagc aucaucgaga aggagcaggu cuccacgacc     2160

gacaaggacc uccagagcau cguggccgac cuggcggggu ccccggccau caagaagggc     2220

auccugcaga gccucaagau cguggacgag cuggugucca ucauggggua cccgccccag     2280

accaucgucg uggagauggc ccgcgagaac cagaccacgg ugaagggcaa gaacaacagc     2340

cggccccgcu acaagucccu ggagaaggcc aucaaggagu ucgggagcca gauccucaag     2400

gagcacccca ccgacaacca ggagcugaag aacaaccggc uguaccucua cuaccugcag     2460

aacggcaagg acaucuacac cggccaggag cuggacaucc acaaccucag caacuacgac     2520

aucgaccaca ucguccccca guccuucauc accgacaaca gcaucgacaa ccuggugcug     2580

accuccagcg ccgggaaccg cgagaagggc ggggacgugc cgccccucga gaucguccgc     2640

aagcggaagg uguucuggga gaagcuguac cagggcaacc ugauguccaa gcgcaaguuc     2700

gacuaccuca cgaaggcgga gcggggcggg cugaccgacg ccgacaaggc ccgcuucauc     2760

caccggcagc ugguggagac ccgccagauc accaagaacg ucgccaacau ccuccaccag     2820

cgguucaaca acgagacgga caacuacggc aacaccaugg agcagguccg caucgugacc     2880

cucaagagcg cgcugguguc ccaguuccgg aagcaguucc agcuguacaa ggugcgcgag     2940

gugaacgacu accaccacgc ccacgacgcc uaccugaacg gggucguggc caacacccug     3000

cucaaggugu acccccagcu ggagcccgag uucgucuacg gcgaguacca ccaguucgac     3060

ugguucaagg ccaacaaggc gacggccaag aagcaguucu acaccaacau caugcuguuc     3120

uucgcccaga aggagcggau caucgacgag aacggggaga uccucuggga caagaaguac     3180

cuggagacca ucaagaaggu gcuggacuac cgccagauga acaucgugaa gaagaccgag     3240

auccagaagg gcgaguucag caaggccacg aucaagccca agggcaacuc cagcaagcuc     3300

aucccccgga aggagaacug ggacccgaug aaguacgggg gccuggacuc ccccaacaug     3360

gccuacgcgg ucaucaucga gcacgccaag gggaagaaga agaucgugau cgagaagaag     3420

cugauccaga ucaacaucau ggagcgcaag auguucgaga aggacgagga ggccuuccuc     3480

gaggagaagg gcuaccggca ccccaaggug cugaccaagc ugcccaagua cacccucuac     3540

gagugcgaga aggggcgccg gcgcaugcug gccagcgcca acgaggcgca gaagggcaac     3600

cagcuggucc ucuccaacca ccuggugagc cugcucuacc acgccaagaa cugcgaggcc     3660

uccgacggca agagccugaa guacaucgag gcccaccggg aggcguucuc cgagcugcuc     3720

gcccagguga gcgaguucgc cacccgcuac acgcuggccg acgccaaccu gagcaagauc     3780

aacaaccucu ucgagcagaa caaggagggg gacaucaagg cgaucgccca guccuucguc     3840

gaccugaugg uguucaacgc caugggcgcc ccggccagcu ucaaguucuu cgaggcgacc     3900

aucgaccgga agcgcuacac caaccugaag gagcuccugu ccagcaccau caucuaccag     3960

uccaucacgg ggcuguacga gagccgcaag cggcucgacg acuga                     4005


<210>  1798
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1798
augaagaacc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacggacc aguacgaccu ggugaagcgc aagaugaagg uggccgggaa cuccgacaag      120

aagcagauca agaagaacuu cuggggcguc cggcuguucg acgaggggga gaccgcggcc      180

gaccgccgga ugaaccgcac cgcccggcgc cggaucgagc gccggcgcaa ccggaucagc      240

uaccuccagg agaucuucgc ccuggagaug gccaacaucg acgcgaacuu cuucugccgc      300

cugaacgacu ccuucuacgu ggacagcgag aagcggaacu cccgccaccc guucuucgcc      360

accaucgagg aggagguggc cuaccacaag aacuaccgga cgaucuacca ccuccgcgag      420

gagcugguca acagcuccga gaaggccgac cugcggcucg uguaccuggc gcuggcccac      480

aucaucaagu accgcggcaa cuuccucauc gagggcgccc uggacaccaa gaacaccagc      540

guggacgggg ucuacaagca guucauccag accuacaacc agguguucau cuccaacauc      600

gaggagggca cgcuggccaa gauggaggag aacaccaccg uggccaacau ccucgcgggg      660

aaguucaccc ggaaggagaa gcuggagcgc auccugcagc ucuaccccgg cgagaagucc      720

acggggaugu ucgcccaguu caucagccug aucgucggca gcaagggcaa cuuccagaag      780

guguucgacc ugguggagaa gaccgacauc gagugcgcca aggacuccua cgaggaggac      840

cucgaggccc ugcuggccau caucggggac gaguacgcgg agcuguucgu cgccgccaag      900

aacaccuaca acgccguggu gcucagcucc aucaucaccg ucacggacac cgagaccaac      960

gccaagcuga gcgcguccau gaucgagcgg uucgacgccc acgagaagga ccucagcgag     1020

cugaaggccu ucaucaagcu gcaccucccc aagcaguacg aggagaucuu cuccaacgug     1080

gccaucgacg gcuacgccgg guacaucgac ggcaagacca agcaggucga cuucuacaag     1140

uaccugaaga cgcugcucga gaacaucgag ggcgcggacu acuucaucgc caagaucgag     1200

gaggagaacu uccugcgcaa gcagcgcacc uucgacaacg gggccauccc ccaccagcug     1260

caccucgagg agcuggaggc cauccugcac cagcaggcga aguacuaccc guuccucaag     1320

gaggccuacg acaagaucaa gagccuggug accuuccgga uccccuacuu cgugggcccc     1380

cuggccaacg ggcagagcga cuucgccugg cucacccgca aggccgacgg cgagauccgg     1440

cccuggaaca ucgaggagaa gguggacuuc gggaaguccg cggucgacuu caucgagaag     1500

augacgaaca aggacaccua ccugccgaag gagaacgugc ugcccaagca cagccucuac     1560

uaccagaagu acauggucua caacgagcug accaaggugc gcuacaucga cgaccagggc     1620

aagaccaacu acuucuccgg ccaggagaag cagcagaucu ucaacgacua cuucaagcag     1680

aagcggaagg ugagcaagaa ggaccuggag caguuccucc gcaacauguc ccacaucgag     1740

agccccacga ucgaggggcu ggaggacucc uucaacagcu ccuacgccac cuaccacgac     1800

cugcugaagg ucggcaucaa gcaggaggug cucgagaacc cccugaacau ggagaugcug     1860

gaggacaucg ugaagauccu caccgucuuc gaggacaagc ggaugaucaa ggagcagcug     1920

cagcaguuca gcggggugcu ggacggcgcc gugcucaaga agcuggagcg ccggcacuac     1980

accggguggg gccgccuguc cgccaagcuc cuggucggca uccgggacaa gcagagccac     2040

cugacgaucc ucgacuaccu gaugaacgac gacgggcuga accgcaaccu caugcagcug     2100

aucaacgacu ccaaccugag cuucaagucc aucaucgaga aggagcaggu cagcaccacc     2160

gacaaggacc uccaguccau cguggccgac cuggcgggca gcccggccau caagaagggg     2220

auccugcagu cccucaagau cguggacgag cuggugagca ucaugggcua cccgccccag     2280

accaucgugg ucgagauggc ccgggagaac cagacgaccg ugaaggggaa gaacaacagc     2340

cgcccccggu acaagucccu ggagaaggcc aucaaggagu ucggcagcca gauccucaag     2400

gagcacccca ccgacaacca ggagcugcgc aacaaccggc uguaccucua cuaccugcag     2460

aacggcaagg acauguacac cgggcaggag cuggacaucc acaaccucuc caacuacgac     2520

aucgaccaca ucgugcccca gagcuucauc accgacaacu ccaucgacaa ccugguccug     2580

acgagcuccg ccggcaaccg cgagaagggg gacgacgugc cgccccucga gaucgugcgg     2640

aagcgcaagg ucuucuggga gaagcuguuc cagggcaacc ugaugagcaa gcgcaaguuc     2700

gacuaccuca ccaaggcgga gcggggcggg cugaccgagg ccgacaaggc caccuucauc     2760

caccgccagc ugguggagac gcggcagauc accaagaacg uggccaacau ccuccaccag     2820

cgcuucaaca acgagaccga caaccacggc aacaacaugg agcagguccg gaucgugaug     2880

cugaaguccg cgcuggugag ccaguuccgc aagcaguucc agcucuacaa gguccgggag     2940

gugaacgacu accaccacgc ccacgacgcc uaccugaacg ggguggucgc caacacccug     3000

cucaaggugu acccccagcu ggagcccgag uucguguacg gcgaguacca ccaguucgac     3060

ugguucaagg ccaacaaggc gacggccaag aagcaguucu acaccaacau caugcuguuc     3120

uucgcccaga aggagcgcau caucgacgag aacggggaga uccucuggga caagaaguac     3180

cuggagacca ucaagaaggu ccuggacuac cggcagauga acaucgugaa gaagaccgag     3240

auccagaagg gcgaguucuc caaggccacg aucaagccca agggcaacag cuccaagcuc     3300

aucccccgca aggagaacug ggacccgaug aaguacgggg gccuggacag ccccaacaug     3360

gccuacgcgg ugaucaucga gcacgccaag gggaagaaga agaucgucuu cgagaagaag     3420

aucauccgga ucaccaucau ggagcgcaag gccuucgaga aggacgagaa guccuuccug     3480

gagaagcagg gcuaccggca gcccaaggug cucaccaagc ugcccaagua cacccuguac     3540

gagugcgaga acgggcgccg gcgcaugcuc gccagcgcca acgaggcgca gaagggcaac     3600

cagcaggucc ugaagggcca gcugaucacg cuccugcacc acgccaagaa cugcgaggcc     3660

uccgacggca agagccugga cuacaucgag agcaaccggg agauguucgg ggagcuccug     3720

gcccacgugu ccgaguucgc gaagcgcuac acccuggccg acgccaaccu cagcaagauc     3780

aaccagcugu ucgagcagaa caaggacaac gacaucaagg ugaucgccca guccuucgug     3840

aaccugaugg ccuucaacgc gaugggggcc ccggccagcu ucaaguucuu cgaggccacc     3900

aucgagcgga agcgcuacac caaccucaag gagcugcugc gggccacgau caucuaccag     3960

uccaucaccg gccucuacga ggcgcgcaag cgccuggacg gguga                     4005


<210>  1799
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1799
augaagaacc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacggacc aguacgaccu ggugaagcgc aagaugaagg uggccgggaa cuccgacaag      120

aagcagauca agaagaacuu cuggggcguc cggcuguucg acgaggggga gaccgcggcc      180

gaccgccgga ugaaccgcac cgcccggcgc cggaucgagc gccggcgcaa ccggaucagc      240

uaccuccagg agaucuucgc ccuggagaug gccaacaucg acgcgaacuu cuucugccgc      300

cugaacgacu ccuucuacgu ggacagcgag aagcggaacu cccgccaccc guucuucgcc      360

accaucgagg aggagguggc cuaccacaag aacuaccgga cgaucuacca ccuccgcgag      420

gagcugguca acagcuccga gaaggccgac cugcggcucg uguaccuggc gcuggcccac      480

aucaucaagu accgcggcaa cuuccucauc gagggcgccc uggacaccaa gaacaccagc      540

guggacgggg ucuacaagca guucauccag accuacaacc agguguucau cuccaacauc      600

gaggagggca cgcuggccaa gauggaggag aacaccaccg uggccgacau ccucgcgggg      660

aaguucaccc ggaaggagaa gcuggagcgc auccugcagc ucuaccccgg cgagaagucc      720

acggggaugu ucgcccaguu caucagccug aucgucggca gcaagggcaa cuuccagaag      780

guguucgacc ugguggagaa gaccgacauc gagugcgcca aggacuccua cgaggaggac      840

cucgaggccc ugcuggccau caucggggac gaguacgcgg agcuguucgu cgccgccaag      900

aacaccuaca acgccguggu gcucagcucc aucaucaccg ucacggacac cgagaccaac      960

gccaagcuga gcgcguccau gaucgagcgg uucgacgccc acgagaagga ccucagcgag     1020

cugaaggccu ucaucaagcu gcaccucccc aagcaguacg aggagaucuu cuccaacgug     1080

gccaucgacg gcuacgccgg guacaucgac ggcaagacca agcaggucga cuucuacaag     1140

uaccugaaga cgcugcucga gaacaucgag ggcgcggacu acuucaucgc caagaucgag     1200

gaggagaacu uccugcgcaa gcagcgcacc uucgacaacg gggccauccc ccaccagcug     1260

caccucgagg agcuggaggc cauccugcac cagcaggcga aguacuaccc guuccucaag     1320

gaggccuacg acaagaucaa gagccuggug accuuccgga uccccuacuu cgugggcccc     1380

cuggccaacg ggcagagcga cuucgccugg cucacccgca aggccgacgg cgagauccgg     1440

cccuggaaca ucgaggagaa gguggacuuc gggaaguccg cggucgacuu caucgagaag     1500

augacgaaca aggacaccua ccugccgaag gagaacgugc ugcccaagca cagccucuac     1560

uaccagaagu acauggucua caacgagcug accaaggugc gcuacaucga cgaccagggc     1620

aagaccaacu acuucuccgg ccaggagaag cagcagaucu ucaacgacua cuucaagcag     1680

aagcggaagg ugagcaagaa ggaccuggag caguuccucc gcaacauguc ccacaucgag     1740

agccccacga ucgaggggcu ggaggacucc uucaacagcu ccuacgccac cuaccacgac     1800

cugcugaagg ucggcaucaa gcaggaggug cucgagaacc cccugaacac cgagaugcug     1860

gaggacaucg ugaagauccu caccgucuuc gaggacaagc ggaugaucaa ggagcagcug     1920

cagcaguuca gcgacgugcu ggacggggcc gugcucaaga agcuggagcg ccggcacuac     1980

acgggcuggg ggcgccuguc cgccaagcuc cuggucggca uccgggacaa gcagagccac     2040

cugaccaucc ucgacuaccu gaugaacgac gacggccuga accgcaaccu caugcagcug     2100

aucaacgacu ccaaccugag cuucaagucc aucaucgaga aggagcaggu cagcaccacc     2160

gacaaggacc uccaguccau cguggccgac cuggcgggga gcccggccau caagaagggc     2220

auccugcagu cccucaagau cguggacgag cuggugagca ucauggggua cccgccccag     2280

acgaucgugg ucgagauggc ccgggagaac cagaccaccg ugaagggcaa gaacaacagc     2340

cgcccccggu acaagucccu ggagaaggcc aucaaggagu ucgggagcca gauccucaag     2400

gagcacccca ccgacaacca ggagcugcgc aacaaccggc uguaccucua cuaccugcag     2460

aacggcaagg acauguacac cggccaggag cuggacaucc acaaccucuc caacuacgac     2520

aucgaccaca ucgugcccca gagcuucauc acggacaacu ccaucgacaa ccugguccug     2580

accagcuccg ccgggaaccg cgagaagggc gacgacgugc cgccccucga gaucgugcgg     2640

aagcgcaagg ucuucuggga gaagcuguuc caggggaacc ugaugagcaa gcgcaaguuc     2700

gacuaccuca ccaaggcgga gcggggcggc cugaccgagg ccgacaaggc cacguucauc     2760

caccgccagc ugguggagac ccggcagauc accaagaacg uggccaacau ccuccaccag     2820

cgcuucaaca acgagaccga caaccacggg aacaacaugg agcagguccg gaucgugaug     2880

cugaaguccg cgcuggugag ccaguuccgc aagcaguucc agcucuacaa gguccgggag     2940

gugaacgacu accaccacgc ccacgacgcc uaccugaacg gcguggucgc caacacgcug     3000

cucaaggugu acccccagcu ggagcccgag uucguguacg gggaguacca ccaguucgac     3060

ugguucaagg ccaacaaggc gaccgccaag aagcaguucu acaccaacau caugcuguuc     3120

uucgcccaga aggagcgcau caucgacgag aacggcgaga uccucuggga caagaaguac     3180

cuggagacca ucaagaaggu ccuggacuac cggcagauga acaucgugaa gaagacggag     3240

auccagaagg gggaguucuc caaggccacc aucaagccca agggcaacag cuccaagcuc     3300

aucccccgca aggagaacug ggacccgaug aaguacggcg ggcuggacag ccccaacaug     3360

gccuacgcgg ugaucaucga gcacgccaag ggcaagaaga aggucguguu cgagaagaag     3420

aucauccgga ucaccaucau ggagcgcaag gccuucgaga aggacgagaa guccuuccug     3480

gagaagcagg gguaccggca gcccaaggug cucaccaagc ugcccaagua cacgcuguac     3540

gagugcgaga acggccgccg gcgcaugcuc gccagcgcca acgaggcgca gaaggggaac     3600

cagcaggucc ugaagggcca gcugaucacc cuccugcacc acgccaagaa cugcgaggcc     3660

uccgacggca agagccugga cuacaucgag agcaaccggg agauguucgg ggagcuccug     3720

gcccacgugu ccgaguucgc gaagcgcuac acccuggccg acgccaaccu cagcaagauc     3780

aaccagcugu ucgagcagaa caaggacaac gacaucaagg ugaucgccca guccuucguc     3840

aaccugaugg ccuucaacgc gaugggcgcc ccggccagcu ucaaguucuu cgaggccacc     3900

aucgagcgga agcgcuacac gaaccucaag gagcugcugu ccgccaccau caucuaccag     3960

agcaucaccg ggcucuacga ggcgcggaag cgccuggacg gcuga                     4005


<210>  1800
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1800
augaagaacc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacgaacc aguacgaccu ggugaagcgc aagaugaagg uggccgggaa cuccgacaag      120

aagcagauca agaagaacuu cuggggcguc cggcuguucg acgacgggca gaccgcggug      180

gaccgccgga ugaaccgcac cgcccggcgc cggaucgagc gccggcgcaa ccggaucagc      240

uaccuccagg agaucuucgc cguggagaug gccaacaucg acgccaacuu cuucugccgc      300

cugaacgacu ccuucuacgu cgacagcgag aagcggaacu cccgccaccc guucuucgcg      360

accaucgagg aggagguggc cuaccacgac aacuaccgga cgaucuacca ccuccgcgag      420

gagcugguga acagcuccga gaaggccgac cugcggcugg ucuaccucgc ccuggcgcac      480

aucaucaagu accgcggcaa cuuccugauc gagggcgccc ucgacaccaa gaacaccagc      540

guggacgagg uguacaagca guucauccag accuacaacc aggucuucau guccaacauc      600

gaggaggggg cccuggccaa gguggaggag aaccuggagg uggccagcau ccucgcgggc      660

aaguucacgc ggcgcgagaa guucgagcgg auccugcgcc uguaccccgg ggagaagucc      720

accggcaugu ucgcccaguu caucagccuc aucgucggga acaagggcaa cuuccagaag      780

guguucaacc ugguggagaa gaccgacauc gagugcgcca aggacuccua cgaggaggac      840

cuggaggccc uccuggccau caucggcgac gaguacgcgg agcuguucgu cgccgccaag      900

aacaccuaca acgccguggu gcucagcucc aucaucacgg ucaccgccac cgagaccaac      960

gcgaagcuga gcgccuccau gaucgagcgc uucgacgccc acgagaagga ccugggggag     1020

cucaaggccu ucaucaagcu gcaccucccc aagcaguacc aggagaucuu caacaacgcc     1080

gcgaucgacg gcuacgccgg guacaucgac ggcaagacga agcaggucga cuucuacaag     1140

uaccugaaga ccacccugga gaacguggag ggcgccgacu acuucaucac caagaucgag     1200

gaggagaacu uccugcggaa gcagcgcacg uucgacaacg gggugauccc ccaccagcuc     1260

caccuggagg agcuggaggc cauccuccac cagcaggcga aguacuaccc guuccugcgg     1320

gaggacuacg agaagaucaa gagccuggug accuuccgca uccccuacuu cgucggcccc     1380

cucgccaagg ggcaguccga guucgccugg cugacccgga aggccgacgg cgagauccgc     1440

cccuggaaca ucgaggagaa gguggacuuc ggcaagagcg ccguggacuu caucgagaag     1500

augaccaaca aggacacgua ccugccgaag gagaacgucc uccccaagca cagccugugc     1560

uaccagaagu acauggugua caacgagcug accaaggugc gguacaucga cgaccagggg     1620

aagaccaacu acuucuccgg ccaggagaag cagcagaucu ucaacgaccu cuucaagcag     1680

aagcgcaagg ucaagaagaa ggaccuggag cuguuccucc ggaacaucaa ccacaucgag     1740

agccccacca ucgaggggcu ggaggacucc uucaacgcga gcuacgccac guaccacgac     1800

cugcugaagg ugggcaugaa gcaggagucc cucgacagcc cccugaacgc cgagaugcug     1860

gaggacaucg ugaagauccu caccgucuuc gaggacaagc gcaugaucaa ggagcagcug     1920

cagcaguucu ccgacgugcu ggacggggug guccucaaga agcuggagcg gcgccacuac     1980

accggcuggg ggcggcugag cgccaagcuc cuggugggca uccgcgacaa gcagucccac     2040

cugaccaucc ucgacuaccu gaugaacgac gacggccuga accggaaccu caugcagcug     2100

aucaacgaca gcaaccuguc cuucaagagc aucaucgaga aggagcaggu cuccacgacc     2160

gacaaggacc uccagagcau cguggccgac cuggcggggu ccccggccau caagaagggc     2220

auccugcaga gccucaagau cguggacgag cuggugucca ucauggggua cccgccccag     2280

accaucgucg uggagauggc ccgcgagaac cagaccacgg ugaagggcaa gaacaacagc     2340

cggccccgcu acaagucccu ggagaaggcc aucaaggagu ucgggagcca gauccucaag     2400

gagcacccca ccgacaacca ggagcugaag aacaaccggc uguaccucua cuaccugcag     2460

aacggcaagg acaucuacac cggccaggag cuggacaucc acaaccucag caacuacgac     2520

aucgaccaca ucguccccca guccuucauc accgacaaca gcaucgacaa ccuggugcug     2580

accuccagcg ccgggaaccg cgagaagggc ggggacgugc cgccccucga gaucguccgc     2640

aagcggaagg uguucuggga gaagcuguac cagggcaacc ugauguccaa gcgcaaguuc     2700

gacuaccuca cgaaggcgga gcggggcggg cugaccgacg ccgacaaggc ccgcuucauc     2760

caccggcagc ugguggagac ccgccagauc accaagaacg ucacgaacau ccuccaccag     2820

cgguucaaca acgagaccga caacuacggc aacaccaugg agcagguccg caucgugacc     2880

cucaagagcg cccugguguc ccaguuccgg aagcaguucc agcuguacaa ggugcgcgag     2940

gugaacgacu accaccacgc gcacgacgcc uaccugaacg gggucguggc caacacgcug     3000

cucaaggugu acccccagcu ggagcccgag uucgucuacg gcgaguacca ccaguucgac     3060

ugguucaagg ccaacaaggc caccgcgaag aagcaguucu acaccaacau caugcuguuc     3120

uucgcccaga aggagcggau caucgacgag aacggggaga uccucuggga caagaaguac     3180

cuggagacca ucaagaaggu gcuggacuac cgccagauga acaucgugaa gaagacggag     3240

auccagaagg gcgaguucag caaggccacc aucaagccca agggcaacuc cagcaagcuc     3300

aucccccgga aggagaacug ggacccgaug aaguacgggg gccuggacuc ccccaacaug     3360

gccuacgccg ucaucaucga gcacgcgaag gggaagaaga agaucgugau cgagaagaag     3420

cugauccaga ucaacaucau ggagcgcaag auguucgaga aggacgagga ggccuuccuc     3480

gaggagaagg gcuaccggca ccccaaggug cugaccaagc ugcccaagua cacccucuac     3540

gagugcgaga aggggcgccg gcgcaugcug gccagcgcca acgaggccca gaagggcaac     3600

cagcuggucc ucuccaacca ccuggugagc cugcucuacc acgcgaagaa cugcgaggcc     3660

uccgacggca agagccugaa guacaucgag gcccaccggg aggccuucuc cgagcugcuc     3720

gcccagguga gcgaguucgc gacgcgcuac acccuggccg acgccaaccu gagcaagauc     3780

aacaaccucu ucgagcagaa caaggagggg gacaucaagg ccaucgcgca guccuucguc     3840

gaccugaugg uguucaacgc caugggcgcc ccggccagcu ucaaguucuu cgaggccacc     3900

aucgaccgga agcgcuacac caaccugaag gagcuccugu ccagcacgau caucuaccag     3960

uccaucaccg ggcuguacga gagccgcaag cggcucgacg acuga                     4005


<210>  1801
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1801
augaagaagg aguacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacggaga acuacgaccu ggugaagaag aagaugaagg uguacgggaa caccgagacc      120

aaguaccuga agaagaaccu cuggggcguc cgccuguucg acgaggggga gaccgccgcg      180

gaccggcgcc ugaagcggac gacccgccgg cgcuacuccc ggcgccggaa ccgcaucugc      240

cggcuccagg accuguucac cgaggagaug aaccaggugg acgccaacuu cuuccaccgc      300

cugcaggaga gcuuccucgu gcccgacgag aaggaguucg agcggcacgc caucuucggc      360

aagauggagg aggaggucuc cuacuaccgc gaguucccga ccaucuacca ccuccggaag      420

caccuggccg acacgagcga gcaggccgac cuccgccugg uguaccuggc gcucgcccac      480

aucgugaagu accggggcca cuuccugauc gagggggagc ugaacaccga gaacuccagc      540

gucuccgaga ccuuccgcac cuucauccag guguacaacc agaucuuccg ggagaacgag      600

gugccccucg ccguccccga caacaucgag gagcuguuca gcgagaaggu gucccgcgcc      660

cggaaggugg aggcgauccu gagcgucuac uccgaggaga aguccacggg cacccucgcc      720

caguuccuga agcugauggu ggggaaccag ggccgcuuca agaagaccuu cgaccucgag      780

gaggacggga ucauccagau ccccaaggag gaguacgagg aggagcugga gacccugcuc      840

gccaucaucg gcgacgagua cgccgagauc uucagcgcca ccaagagcgu guacgacgcg      900

gucgcccugu ccggcauccu gagcgugacg gacggggaca ccaaggccaa gcucuccgcc      960

agcauggugg agcgcuacga ggcccaccag aaggaccugg uccaguucaa gcaguucauc     1020

cggaaggagc ugccggagau guacgcgccc aucuuccgcg acaacuccgu gagcggcuac     1080

gccggguacg uggagaacag caaggucgug acccaggccg aguucuacaa guacaucaag     1140

aaggccaucg agaagguccc cggcgccgag uacuuccucg agaagaucga gcaggagacg     1200

uuccuggaca agcagcggac cuucaacaac ggcgugaucc cccaccagau ccaccuggag     1260

gagcucgagg cgaucaucca gaagcaggcc accuacuacc cguuccuggc cgacaacaag     1320

gaggagauga agcagcuggu gaccuuccgc auccccuacu acgucgggcc ccucgccgac     1380

ggcaacuccc ccuucgccug gcuggagcgg aucagcuccg agccgauccg ccccgggaac     1440

cuggcggagg ugguggacau caagaagucc gccacgaagu ucaucgagcg gaugaccaac     1500

uucgacaccu accuccccac cgagaagguc cugcccaagc acagcaugau cuacgagaag     1560

uacauggugu acaacgagcu gacgaaggug agcuacgucg acgagcgcgg caugaaccag     1620

cgguucuccg gggaggagaa gaagcagauc guggaggagc uguucaagca gagccgcaag     1680

gugaccaaga agcugcugga gaaguuccuc uccaacgagu ucggccuggu cgacguggcc     1740

aucaagggca ucgagaccag cuucaacgcc ggguacggca ccuaccacga cuuccugaag     1800

aucgggauca cgcgggagca gcuggacaag gaggagaacu ccgagacccu cgaggagauc     1860

gugaagaucc ugaccgucuu cgaggaccgc aagaugaucc gggagcagcu gaagaaguac     1920

accuaccucu ucgacgagga ggugcugaag aagcuggagc gccggcacua cacgggcugg     1980

gggcgccuca gcgcgaagcu gcugaucggc aucaaggaga agcggaccca caagaccauc     2040

cucgacuacc ugaucgagga cgacggcggg aagcagccga ucaaccgcaa ccugaugcag     2100

cucaucaacg acuccgaccu gagcuucaag uccgagaucg ccgaggccca gagcgacaug     2160

aacaccgagg accugcacga ggugguccag aaccucgccg gcucccccgc caucaagaag     2220

gggauccugc agagccugaa gaucguggac gagcucgugg acaucauggg cucccugccc     2280

aagaacaucg ucguggagau ggcgcgggag aaccagacca cgagccgcgg gcgcaccaac     2340

agcaaccccc ggaugaaggc ccuggaggag gccaugcgca accuccgguc caaccugcug     2400

aaggaguacc cgaccgacaa ccaggcccuc cagaacgacc gccuguaccu guacuaccuc     2460

cagaacggca aggacaugua caccggccug gaccugagcc uccacaaccu guccagcuac     2520

gacaucgacc acaucgugcc ccaguccuuc acgaccgaca acagccugga caaccggguc     2580

cucgugucca gcaaggagaa ccgcgggaag aaggacgacg ugcccuccaa ggaggucgug     2640

cagaagaaca ucacccugug ggagacccug aagaacagca accucaucuc ccagaagaag     2700

uacgacaacc ugacgaaggg ccugcgcggg ggccucaccg aggacgaccg ggcccacuuc     2760

aucaagcggc agcuggugga gacccgccag aucaccaagc acgucgcgcg gauccuggac     2820

cagcgcuuca acagccagaa ggacgaggag ggcaagacga uccgggccgu gcgcgugguc     2880

acccucaagu ccagccugac cucccaguuc cggaagcagu ucgccaucca caaggugcgc     2940

gagaucaacg acuaccacca cgggcacgac gccuaccuga acggcguggu cgccaacagc     3000

cuccugcggg uguaccccca gcugcagccg gaguucgugu acggggacua ccccaaguuc     3060

aacgcguaca aggccaacaa ggccaccgcc aagaagcagc ucuacacgaa caucaugaag     3120

uucuucgcgg aggacgccgu caucaucgac gagaacggcg agauccugug ggacaagaag     3180

aacaucgcca ccgugaagaa ggugaugucc uacccccaga ugaacaucgu caagaagccc     3240

gagauccaga ccgggagcuu cagcaaggag accaucaagc cgaagggcga cuccgacaag     3300

cugaucagcc gcaagacgaa cugguccccc aagcucuacg gcggguucga cagcccccag     3360

guggccuacu ccgugaucau caccuacgag aagggcaaga agaagguccg ggccaaggcg     3420

aucgugggga ucaccaucau ggagcagagc cuguucaaga aggacccggu gucccugcuc     3480

gaggagaagg gcuacgccaa ccccgagguc cugauccacc uccccaagua cacccuguac     3540

gagcuggaga acgggcgccg gcgccugcuc gccagcgcca acgaggccca gaagggcaac     3600

cagcuggugc ugcccgcguc ccucgugacg cugcuguacc acgccaagca ggucgacgag     3660

gacagcggca aguccgagga guacgugcgc gagcaccggg ccgaguucgc cgagauccuc     3720

aacuacgucc aggccuucag cgagaccaag auccuggcga acaagaaccu gcagaccauc     3780

cucaagcugu acgaggagaa caaggaggcc gacaucaagg agaucgccga guccuucgug     3840

aaccugauga aguucagcgc cuacggggcg ccgauggacu ucaaguucuu cggcaagacc     3900

aucccccgcu cccgguacac gagcgugggg gagcuccugu ccgccaccau caucaaccag     3960

agcaucaccg gccuguacga gacccgccgg aagcucgucg acuga                     4005


<210>  1802
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1802
augcgcaagc ccuacaccau cggccuggac aucgggacca acagcguggg cugggccguc       60

cucacggacc aguacaaccu ggugaagcgg aagaugaagg uggccggguc cgccgagaag      120

aagcagauca agaagaacuu cuggggcguc cgccuguucg acgaggggga gguggccgcc      180

ggccggcgca ugaaccggac cacccgccgg cgcaucgagc ggcgccggaa ccgcaucgcc      240

uaccuccagg agaucuucgc cgcggagaug gccgaggugg acgccaacuu cuucuaccgg      300

cuggaggaca gcuucuacau cgaguccgag aagcgccaca gccggcaccc guucuucgcc      360

accaucgagg aggaggucgc guaccacgag gaguacaaga cgaucuacca ccuccgcgag      420

aagcugguga acuccagcga caaggccgac cugcggcugg uguaccucgc ccuggcccac      480

aucaucaagu accgcggcaa cuuccugauc gaggggaugc ucgacaccaa gaacaccucc      540

gucgacgagg uguucaagca guucauccag accuacaacc agaucuucgc cagcgacauc      600

gaggagggcu cccugacgcg gcuggaggag aacaaggagg uggcggagau ccucagcgag      660

aagcugaccc gccgggagaa gcuggacaag auccucaagc uguacaccgg ggagaagucc      720

accggcaugu ucgcccgcuu caucaaccug aucaucggga gcaagggcga cuucaagaag      780

gucuucgacc ucgacgagaa ggccgagauc gagugcgcca aggacacgua cgaggaggac      840

cuggaggccc ugcucgcgaa gaucggcgac gaguacgccg agaucuucgu ggccgccaag      900

uccaccuaca acgccguggu ccugagcaac aucaucaccg ugaccgacac ggagaccaag      960

gcgaagcugu ccgccagcau gaucgagcgc uucgacaagc acgccaagga ccucaagcgg     1020

cugaaggccu ucuucaagau gcagcugccc gagaaguuca acgagguguu caacgacauc     1080

gagaaggacg gguacgccgg cuacaucgac gggaagacca cccaggagaa guucuacaag     1140

uacaugaaga agaugcucgc gaacaucgac ggcgccgacu acuucaucga ccagaucgag     1200

gaggagaacu uccugcgcaa gcagcggacg uucgacaacg gcaccauccc ccaccagcug     1260

caccucgagg agcuggaggc cauccugcac cagcaggcca aguacuaccc cuuccuccgc     1320

aaggacuacg agaagauccg gucccugguc accuuccgca ucccguacuu caucgggccc     1380

cuggcgaacg gccagagcga cuucgccugg cucacccgga aggccgacgg ggagauccgc     1440

cccuggaaca ucgaggagaa gguggacuuc ggcaaguccg ccaucgacuu caucgagaag     1500

augacgaaca aggacaccua ccugcccaag gagaacgugc ugccgaagca cagccucugc     1560

uaccagaagu acauggucua caacgagcug accaagaucc gguacaucga cgaccagggg     1620

aagacccacc acuucagcgg ccaggagaag cagcagaucu ucaacggccu guucaagcag     1680

cagcgcaagg ugaagaagaa ggaccucgag cgguuccugu acacgaucaa ccacaucgag     1740

ucccccacca ucgagggggu ggaggacgcc uucaacagcu ccuucgcgac cuaccacgac     1800

cugcagaagg gcggggucac ccaggagauc cucgacaacc cccugaacgc cgacaugcug     1860

gaggagaucg ugaagauccu gacggucuuc gaggacaagc gcaugaucaa ggagcagcuc     1920

cagagcuucu ccgacgugcu ggacggcacc auccugaaga agcucgagcg gcgccacuac     1980

accggguggg gccggcugag cgccaagcug cucaccggca uccgcgacaa gcacucccac     2040

cugacgaucc uggacuaccu caugaacgac gacgggcuga accggaaccu gaugcagcuc     2100

aucaacgaca gcaaccuguc cuucaagagc aucaucgaga aggagcaggu cuccaccgcc     2160

gacaagggca uccagagcau cguggccgag cuggcggggu cccccgccau caagaagggc     2220

auccuccaga gccugaagau cguggacgag cuggugggga ucaugggcua cccgccccag     2280

accaucgucg uggagauggc ccgcgagaac cagaccaccg gcaaggggaa gaacaacucc     2340

aagccccggu ucaucagccu cgagaaggcc aucaaggagu ucggcuccca gauccugaag     2400

gagcacccca cggacaacca gugccugaag aacgaccgcc ucuaccugua cuaccugcag     2460

aacgggaagg acauguacac cggcaaggag cucgacaucc acaaccugag caacuacgac     2520

aucgaccaca ucaucccgca guccuucauc accgacaaca gcaucgacaa ccgcgugcug     2580

gucagcucca ccgccaaccg ggagaagggc gacaacgugc cccuccugga ggugguccgc     2640

aagcggaagg cguucuggga gaagcuguac caggccaagc ucaugagcaa gcgcaaguuc     2700

gacuaccuga cgaaggccga gcgcgggggc cugaccgagg ccgacaaggc gaacuucauc     2760

cagcggcagc ucguggagac ccggcagauc accaagaacg uggccaacau ccuguaccag     2820

cgcuucaacu gcaagcagga cgagaacggg aacgaggucg agcagguccg gaucgugacg     2880

cugaagucca cccucgugag ccaguuccgc aagcaguucc agcuguacaa ggugcgggag     2940

gugaacgacu accaccacgc ccacgacgcc uaccugaacg gcgucguggc caacacccuc     3000

cugaaggugu acccccagcu ggagcccgag uucgucuacg gggacuacca ccaguucgac     3060

ugguucaagg cgaacaaggc caccgccaag aagcaguucu acacgaacau caugcgcuuc     3120

uucgccaagg agaaccagau caucgacaag aacggcgaga uccucuggga caaccgguac     3180

cuggacacca ucaagaaggu gcuguccuac cgccagauga acaucgugaa gaagaccgag     3240

auccagaagg gcgaguucag caacgccacc gucaacccga aggggaacuc cagcaagcuc     3300

aucucccgga aggcggacug gaaccccauc aaguacggcg gguucgacgg cagcaacaug     3360

gccuacucca ucgugaucga guacgagaag cgcaagaaga agacggugau caagaaggag     3420

cugauccaga ucaacaucau ggagcggguc gccuucgaga aggaccagaa ggccuuccug     3480

gaggagaagg gguacuacag ccccaaggug cucaccaaga uccccaagua cacccuguac     3540

gagugcgaga acggccgccg gcgcaugcug ggcuccgcca acgaggcgca gaaggggaac     3600

cagauggugc ucccgaacca ccugaugacc cugcucuacc acgccaagaa cugcgaggcc     3660

aacgacggcg agagccuggc cuacaucgag augcaccggg agauguucgc ggagcugcuc     3720

gccuacaucu ccgaguucgc caagcgcuac acgcuggcca acgaccgccu ggagaagauc     3780

aacauguucu ucgagcagaa caagaagggg gacaucaagg ucaucgccaa gagcuucgac     3840

aagcucaagg uguucaacgc guucggcgcg ccccgggacu ucgaguucuu cgagaccacc     3900

aucaagcgca agcgguacua caacaucaag gagcugcuga acgccaccau caucuaccag     3960

uccaucacgg ggcucuacga ggcccgcaag cggcuggagg acuga                     4005


<210>  1803
<211>  4008
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1803
augaagaacu acaccaucgg ccuggacauc gggaccaaca gcgugggcug ggccgucauc       60

aaggacgacc ucacgcuggu gcgcaagaag aucaagaucu ccgggaacac cgacaagaag      120

gaggugaaga agaaccugug gggcagcuuc cucuucgagc agggggacac cgcccaggac      180

acccggguca agcgcaucgc gcggcgccgg uacgagcgcc ggcgcuuccg gauccgcgag      240

cugcagaaga ucuucgacaa guccaugggc gagguggaca gcaacuucuu ccaccggcug      300

gacgaguccu uccucgugga ggaggacaag gaguacagca aguaccccau cuucuccaac      360

gagaaggagg acaagaacua cuacgacaag uacccgacga ucuaccaccu ccgcaaggac      420

cuggccgaca gcaaccagaa ggccgaccuc cggcugaucu accuggcccu cgcccacaug      480

aucaaguacc gcggccacuu ccugaucgag ggggaccuga agauggacgg caucuccauc      540

agcgaguccu uccaggaguu caucgacagc uacaacgagg ucugcgcgcu cgaggacgag      600

aaccuggaga ucaucuacaa cgacgagcug cucacccaga ucgagaacau cuucaagcag      660

gacaucuccc ggagcaagaa gcuggaccag gccaucgccc uguuccaggg ggugaagcgc      720

cagucccucu ucggcaucuu ccugacccug aucgugggga acaaggccaa cuuccagaag      780

aucuucaacc ucgaggacga caucaagcug gaccugaagg aggaggacua cgacgagaac      840

cucgaggagc ugcugagcaa caucgacgag ggcuaccggg acgucuuccu ccaggcgaag      900

aacguguaca acgccaucga gcuguccaag auccugaaga ccgacggcaa ggagacgaag      960

gcgccccuca gcgcccagau gguggagcug uacaaccagc accgcgagga ccugaagaag     1020

uacaaggacu acaucaaggc cuaccucccc gagaaguacg gggagaccuu caaggacgcc     1080

accaagaacg gcuacgccgg guacaucgac ggcaagaccu cccaggagga cuucuacaag     1140

uucgucaagg cccagcugaa gggcgaggag aacggggagu acuuccugga ggccaucgag     1200

aacgagaacu uccuccggaa gcagcgcagc uucuacaacg gcgugauccc cuaccagauc     1260

caccuccagg agcugacggc cgugcuggac cagcaggaga agcacuacag cuuccugaag     1320

gagaaccggg acaagaucau cucccugcuc accuuccgca ucccguacua cgucgggccc     1380

cuggcgaagg gcgagagccg cuucgccugg cuggagcggu ccaacagcga ggagaagauc     1440

aagcccugga acuucgacaa gaucguggac aucgacaagu ccgccgagcu guucaucgag     1500

aaccugacca gccgcgacac cuaccugccc gacgagccgg ugcugcccaa gcggucccuc     1560

aucuaccaga aguucacgau cuucaacgag cugaccaaga ucagcuacau cgacgagcgc     1620

gggauccugc agaacuucuc cagccgggag aagaucgcca ucuucaacga ccucuucaag     1680

aacaagucca aggucaccaa gaaccagcug gugaaguaca ucgagaacaa ggagcagauc     1740

aucgcccccg agaucaaggg caucgaggac agcuucaacu ccaacuacag caccuacauc     1800

gaccugucca agauccccga caugaagaag cuccuggaga aggacgagga cgagauccug     1860

gaggagauca ucaagauccu cacgaucuuc gaggaccgca agaugcggaa gcgccagcug     1920

augaaguuca aggacaagcu gagcgagaag gcgaucaacc agcucuccaa gaagcacuac     1980

accggcuggg ggcagcugag cgagaagcug aucaacggca uccgggacga gcaguccaac     2040

aagaccaucc ucgacuaccu gaucgaggac aacgggugcc cgaagaacau gaaccgcaac     2100

uucaugcagc ugaucaacga cgacacccuc agcuucaagg agaagauccg gaaggcccag     2160

gacaucaacc aggugaacga caucaaggag aucgucaagg accugcccgg cagccccgcc     2220

aucaagaagg ggaucuacca guccauccgc aucguggacg agaucauccg gaagaugaag     2280

gaccgcccca agaacaucgu gaucgagaug gcccgggaga accagacgac ccaggagggc     2340

aagaacaaga gcaaggcccg ccugaagaag auccaggagg gccucgagaa ccuggacucc     2400

guccacgugg agaagcaggc gcuggacgag gagaugcuca agagcccgaa guacuaccug     2460

uacugccugc agaacgggaa ggacaucuac accggcaagg accucgacau cgggcagcug     2520

cagaccuacg acaucgacca caucaucccc cgguccuuca ucacggacaa cagcuucgac     2580

aaccuggucc ucaccuccag caccgugaac cgcggcaagc uggacaacgu gcccuccccc     2640

gacaucgucc ggcagcagaa gggguucugg aagcagcugc uccgcgccgg ccugaugagc     2700

cagcgcaagu ucaacaaccu gaccaagggc aagcucacgg accgggaccg ccagcaguuc     2760

aucaaccggc agcuggugga gacccgccag aucaccaagc acguggccaa ccugcucucc     2820

caccaccuga acgagaagaa ggaggucggg gagaucaaca ucgugcugcu caagagcgcc     2880

cugaccuccc aguuccggaa gaaguucgac uucuacaagg ugcgcgaggu caacgacuac     2940

caccacgcgc acgacgccua ccugaacggc gugaucgccc ucaagcugcu ggagcucuac     3000

ccguacaugg ccaaggaccu gaucuacggg aaguacagcu accaccggaa gaucgagggc     3060

gacaaggcca cgcaggcgaa guacaagaug uccaacauca ucgagcgcuu cagccaggac     3120

cugcucgcca accccgacgg cgagaucgcc ugggagaagg acaaggaccu gaacaccauc     3180

cggaaggugc uguccagcaa gcagaucaac aucaucaaga aggccgagga ggggaagggc     3240

cgccucuuca aggagaccau caacagccgg cccuccaaga agaccgagaa gcgcaucccc     3300

aucaagaaca accuggaccc gaacaucuac gggggcuaca ucgaggagaa gauggccuac     3360

uacaucgcga ucaacuaccu ggagaacggg aagaccaaga aggccaucgu cggcaucagc     3420

aucaaggaca agaaggacuu cgagggccag acgaccgagu accucgggaa gaucggcuuc     3480

aacaaggccu ccaucaucaa cagcuucaag aacuacaccc uguucgagcu ggagaacggg     3540

ucccggcgca ugaucguggg cgccagcaag gagaacgacu ccaaggggga gcuccagaag     3600

ggcaaccaga uguaccugcc ccagaaccug cucgaguucg uguaccaccu gaagcacuac     3660

aacgaggacg agaccagcca caaguucauc gucgagcaca aggccuacuu cgacgagcug     3720

cucaacuaca ucguggaguu cgcgaacaag uaccuggagc uggagaacuc caucgagaag     3780

aucaaggacc ucuaccacgg caaggggccc gacguggagg agaaggagcu ggucgagagc     3840

uucaucaacc ugcucgccau cacgaagugc ggccccgccg ccgacaucac cuuccugggg     3900

gagaagaucu cccggaagcg cuaccggagc accaacugcc uguggggcuc cgaggugauc     3960

uuccagagcc cgaccgggcu cuacgagacg cgccugcggc uggaguga                  4008


<210>  1804
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1804
auggacgaca acuaccagcu gaugcacgcc aagggcaagu acggguacgg cacccgcauc       60

uacagcgagg ggcagaccgc cgcggaccgg cgcggcuucc ggacgacccg ccggcgccuc      120

aaccggcgca aguggcggcu gcgccugcuc caggacuucu ucgagcccua cauccugccg      180

caggacgagg ccuucuucau ccggcagaag caguccaacc uggugcccca ggacaccaag      240

aagcaguacc ccgagagcgu ccucuucgac gacaccuccg acaaggacuu cuacacggag      300

uaccccacca ucuaccaccu ccgccaggcc cugaugaccg agaaccggca guucgacauc      360

cgcgaggugu accuggccau ccaccacauc gugaaguacc gggggcacuu ccugaacaac      420

gccccgguca agaccuucaa gagcggcaac cuggaccucg cgaucaaguu caacgagcug      480

aacgaggagu ucaaguccgu guaccccgac cuggagcucc aguucaaccu ggaccagauc      540

gacgagauca agaagauccu gcucgacagc acgaaguccc gcuucgaccg gcagggcgag      600

gccgugaagc ugaucuacac cgucagcgag gugaagacca ucgagaagcu gcagaacaag      660

aucgugaagc aguuccucuc cgggauccug ggccugaaca cccgcuucga cguccucacg      720

aacaccgaga gcgacgacuc cauguggaag uucagccugg acuccgagag cuccgacgac      780

gcccuggacg agaucaucgc ccagcucgac gaccccgcga uccacguggu ggagcugguc      840

aacgagaucc acagcuccau cacccuggcc gggaucgugc ccgagggcca ggggcucagc      900

gaguccaugg uggagaagua cgaggcccac aaggcccacc uccagcugcu gaaggccguc      960

gagaagcugg uggacaagaa ggcggccgac aagcuggagg uggccuacga ccaguacauc     1020

aacggcaagg acagcaagca guucaucaag gaggacuucu acaaggccgu ccagagcgag     1080

aucaagggcg uggccgggga cgagguggcg gagaucaacc agcucaucga gcuggaccag     1140

uucaugccga agcagcggac caaggagaac ggcgccaucc cccaccagcu gcaccagcac     1200

gagcucgacg ucaucaucga gaaccagucc aaguacuacg acuggcuggg ggagaaggug     1260

ggcaacgagu acaagcugga caagcucgug agcuuccgcg uccccuacua cgugggcccc     1320

cuggucgaca cggccuccaa ggagaagaag gacgagaccc gguucgccug gauggugcgc     1380

aagaacccgg agagcaccga ggccaucacc cccuggaacu ucgaggacau cguggacaag     1440

aacgcguccg ccgagaacuu caucaagcgc augacgacca aggacaagua ccugcucaac     1500

gaggacgucc ugcccaagug cagccugcuc acccaggacu ucgaggugua caacgagcug     1560

aacaacaucc gggugaacgg ggagaaguuc accgccgagc agaagcagcu gauccacgac     1620

gagcuguuca cgaagcacgc cuccgucagc aucaagcagc ucaaggacuu ccuggugucc     1680

gagcugaugu acgugcgcga caaccucaag gucgagggcc uggccgacga gaagaagcug     1740

augagcuccc ucagcaccca guucgacuac cagaagauca uccccgacca gaucaacgac     1800

gugaaguacc gggaggacau cgagcagauc aucgaguggu ccaccaucuu cgaggacgcg     1860

gccaucuuca agaccaagcu gaaggagauc gacuggcuga cggacgagca gcgcaacaag     1920

cucgccaaca agcgguaccg cggguggggc agccugucca agcggcugcu cgccugccug     1980

guggacgaga acgggcagcg caucaucgac auccugcggg acagcuccga caacuucaug     2040

augaucguca accgcccgga guucaagcag gcgaucuucg acgagaacgc caaccucacc     2100

gagaccaaca gcaagaagga cauguucgag augaucgaca acuucuacac cagcccccag     2160

aacaagaagg ccauccgcca ggugcugcug gugguccagg acauccagaa cgccaugggc     2220

ggcguggcgc cccagaagau cuucaucgag uucgcccggg aggacgaggu gaacgggcgg     2280

cgcacgaacu cccgguacag ccagauccgc gagauguacu ccaaggucac cgacgacgug     2340

aucaacaaga ccgugcgggg cgagcucgag gccaacaagg ggagccugaa caaggagaag     2400

cuguuccucu acuuccugca gggcgggauc gaccuguacu ccggcaagac caucaacauc     2460

gacaacauca gcuccuacga cgucgaccac gugcuccccc agagcuucau caaggacgac     2520

uccaucaaca acaaggugcu ggucaacgcc aagggcaacc gcgugaagaa cgacaacgug     2580

ccgauggagc uguucggggc caagauguac cccacgugga agcggauggu cgacaccggc     2640

aucaugagca aggccaaguu caagaaccuc acccugaacc ccgacaacgu ggacaaguuc     2700

aaggcggagg gguucaucaa ccgccagcug guggagaccc gccagaucau caagcucguc     2760

gcccagaucc ugaacgacaa gcugggcgac gacaccaaga ucguguccgu gaaggccaac     2820

cucacgcacg acaugcgggu cuucuucgac uuccccaaga accgcaacgu gaacaacuac     2880

caccacgccu ucgacgccua ccugagcgcg uucgugggga ccuaccugua caagcgguac     2940

ccgaagcucc agcccuacuu cgucuacggc gaguucaaga agauggacaa gcugaagaac     3000

augaccuccu ucgacuuccu gcgcgaccuc aagcacaccg acaagguggu ggacaaggac     3060

acgggcgaga ucaucgccga caagcuggcc gucaucacca acaugaacaa gaucuacgac     3120

uucaagaagg ugcuggugac ccacgagguc cacgagaaca gcggggccau guacgaccag     3180

acccucuacc ccgcgaagcu ggacaagucc cugggccgga agggggccaa gcagcucauc     3240

cccaagaagg acgacaugcc gacggcccug uacggcggcu acaccgggag caaggacgcc     3300

uucaugucca ucgugcgccu gaagaagaag gacgaggugu acuacaaggu caucggcauc     3360

cccgugcgga ucgccgacag cgugcagcac gggaaggagg ucgacgugga ggcgcucacc     3420

caguucaucg agaccaaguu cacgaaggag aaccugaaca agaagaccgg cgagaucacc     3480

cgcaaggugg agugguucga gcugguccuc cccaaggugc uguucgggca ggccgucuuc     3540

gacggcggcc agccguucau gcuggggucc agcaccuacc cccacaaccu ccgggagcug     3600

gugcugacga aggacagcgu gaagaagauc gccggcaagg aguccaacaa gagcuaccuc     3660

gacgccuacg acgagaucau ggaggccaug aacaaguacu ucccccugua cgacaccaac     3720

aaguuccgcg agaaggucau cgaggggcgg uccaaguuca ucgaccugcc cgacaaggac     3780

gaguacgaga acaacaagcu cguccaggug ggcaagagca ccaucaucga ccgcauguuc     3840

aucgggcugc acgcgaacgc cgcccggacc gaccugaaga acaucggcau cacgaccccg     3900

uucggcuuca ugcagcaggc cuccgggauc aagcucacca aggacaccgu ggugcuguuc     3960

cagagcucca cgggccuguu cgagcgcaag guggcccuca aggaccugug a              4011


<210>  1805
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1805
augaccaagg acuacaccau cggccuggac aucgggacga acagcguggg cugggccguc       60

cucaccgacg acuaccagcu gaugaagcgc aagauguccg ugcacgggaa caccgagaag      120

aagaagauca agaagaacuu cuggggcgcc cggcuguucg acgaggggca gaccgcggag      180

uuccgccgga cgaagcgcac caaccggcgc cggcucgccc gccggaagua ccgccugagc      240

aagcugcagg accucuucgc cgaggagcug ugcaagcagg acgacugcuu cuucgugcgg      300

cuggaggagu ccuuccucgu ccccgaggag aagcaguaca agccggccag caucuucccc      360

acccuggagg aggagaagga guacuaccag aaguacccca ccaucuacca ccuccgccag      420

aagcuggugg acuccacgga gaaggaggac cugcggcugg uguaccucgc ccuggcgcac      480

cuccugaagu accgcggcca cuuccuguuc gagggcuacc uggacaccga gaacaccagc      540

aucgaggagu ccuuccgggu cuuccucgag caguacggga agcagagcga ccagccccug      600

aucgugcacc agccggugcu gaccauccuc acggacaagc uguccaagac caagaagguc      660

gaggagaucc ugaaguacua ccccaccgag aagaucaaca gcuucuucgc ccagugccuc      720

aagcugaucg ugggcaacca ggccaacuuc aagcgcaucu ucgaccugga ggccgaggug      780

aagcuccagu ucuccaagga gaccuacgag gaggaccugg agagccugcu cgagaagauc      840

ggggacgagu accuggacau cuuccugcag gcgaagaagg uccacgacgc cauccuccug      900

uccgagauca ucagcuccac ggugaagcac acccaggcca agcugagcuc cggcauggug      960

gagcgguacg agcgccacaa ggccgaccuc gccaaguuca agcaguucgu caaggagaac     1020

gugccccaga aggcgaccgu guucuucaag gacaccacga agaacgggua cgccggcuac     1080

aucaagggca agaccaccca ggaggaguuc uacaaguucg ucaagaagga gcugagcggg     1140

guggugggcu ccgagcccuu ccuggagaag aucgaccagg agaccuuccu ccugaagcag     1200

cggacguaca ccaacggggu caucccgcac caggugcacc ugaucgagcu caaggccauc     1260

aucgaccagc agaagcagca cuaccccuuc cuggaggagg ccggccccaa gaucaucgcc     1320

cuguucaagu uccgcauccc cuacuacgug ggcccgcucg cgaaggagca ggaggccagc     1380

agcuucgccu ggaucgagcg gaagaccgcc gagaagaucc accccuggaa cuucuccgag     1440

gucguggaca ucgagaaguc cgccaugcgc uucauccagc gcaugaccaa gcaggacacg     1500

uaccugccca ccgagaaggu ccugcccaag aacagccugc ucuaccagaa guacaugauc     1560

uucaacgagc ugaccaaggu gagcuacaag gacgagcgcg gggugaagca guacuucucc     1620

ggcgacgaga agcagcagau cuucaagcag cuguuccaga aggagcgggg gaagaucacc     1680

gucaagaagc uccagaacuu ccuguacacg cacuaccaca ucgagaacgc gcacaucuuc     1740

ggcaucgaga aggccuucaa cgccagcuac uccaccuacc acgacuucau gaagcuggcc     1800

aagaccaacc agaaguccau gcaggagugg cucgagcagc cggagaugga gcccaucuuc     1860

gaggacaucg ugaagauccu gaccaucuuc gaggaccggc agaugaucaa gcaccagcug     1920

agcaaguacc aggagguguu cggggagaag cuccugaagg aguucgcccg caagcacuac     1980

acgggcuggg gccgguucag cgcgaagcug auccacggga uccgcgaccg gaagaccaac     2040

aagaccaucc ucgacuaccu gaucaacgac gacgacgucc ccgccaaccg caaccggaac     2100

cugaugcagc ucaucaacga cgagcaccug uccuucaagg aggagaucgc caaggccacc     2160

guguucagca agcacaaguc ccugguggac gucauccagg accuccccgg cagcccggcg     2220

aucaagaagg ggaucuggca gucccugaag aucguggagg agcugaucgc caucaucggc     2280

uacaagccca agaacaucgu gaucgagaug gcccgcgaga accagaagac ccaccggacg     2340

aagccccgcc ucaaggcccu ggagaacggg cugaagcaga ucggcagcac ccuccugaag     2400

gagcagccca ccgacaacaa ggcccugcag aaggagcggc ucuaccugua cuaccugcag     2460

aacggccgcg acaucuacac cggggagccg cucgacaucg agaaccugca ccaguacgag     2520

gucgaccaca ucaucccccg guccuucauc guggacaaca gcaucgacaa caaggugcug     2580

gucgcgucca agcagaacca gaagaagcgc gacgacgugc ccaacaagca gaucgugaac     2640

gagcagcgga ucuucuggaa ccagcucaag gaggccaagc ugaucagccc caagaaguac     2700

gccuaccuga cgaagaucga gcucaccccg gaggacaagg cccgcuucau ccagcggcag     2760

cuggucgaga cccgccagau caccaagcac guggccaaca uccugcacca gagcuuccac     2820

caggaggagg agggcacgga cugcgacggg gugcagauca ucacccucaa ggcgacccug     2880

accucccagu uccgccagac guucggccug uacaaggucc gggagaucaa cccccaccac     2940

cacgcccacg acgccuaccu caacggguuc aucgccaacg ugcugcugaa gcgcuacccc     3000

aagcucgccc cggaguucgu guacggcaag uacgucaagu acagccuggc gcgggagaac     3060

aaggccaccg ccaagaagga guucuacucc aacauccuga aguuccucga gagcgacgag     3120

cccuucugcg acgagaacgg cgagaucuac ugggagaagu ccuaccaccu cccccgcauc     3180

aagaaggugc ugagcuccca ccaggugaac gucgugaaga agguggagca gcagaagggc     3240

ggguucuaca aggagaccgu caacagcaag gagaagcccg acaagcugau cgagcggaag     3300

aacaacuggg acgugaccaa guacgggggc uucggcuccc cggugaucgc cuacgcgauc     3360

gccuucgucu acgccaaggg gaagacgcag aagaagaccc gcgccaucga gggcaucacc     3420

aucauggagc aggccgcguu cgagaaggac cccaccacgu uccugaagga caagggguuc     3480

ccccagguga ccgaguucau caagcugccc aaguacaccc ucuucgaguu cgacaacggc     3540

cggcgccggu uccuggccag ccacaaggag ucccagaagg ggaacccguu cauccugagc     3600

gaccagcucg ugacccugcu guaccacgcc cagcacuacg acaagaucac guaccaggag     3660

uccuucgacu acgucaacac ccaccucagc gacuucuccg ccauccugac cgaggugcug     3720

gccuucgcgg agaaguacac ccucgccgac aagaacaucg agcgcaucca ggagcuguac     3780

gaggagaaca aguacggcga gaucagcaug aucgcccagu ccuuccugca gcuccugcag     3840

uucaacgcca ucggcgcccc cgcggacuuc aaguucuucg gggugacgau cccccggaag     3900

cgcuacacca gccugaccga gaucugggac gccaccauca ucuaccagag cgucacgggc     3960

cucuacgaga cccggauccg caugggggac cugugggccg gcgagcagug a              4011


<210>  1806
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1806
augaccaagg acuacaccau cggccuggac aucgggacga acagcguggg cugggccguc       60

cucaccgacg acuaccagcu gaugaagcgc aagauguccg ugcacgggaa caccgagaag      120

aagaagauca agaagaacuu cuggggcgcc cggcuguucg acgaggggca gaccgcggag      180

uuccgccgga cgaagcgcac caaccggcgc cggcucgccc gccggaagua ccgccugagc      240

aagcugcagg accucuucgc cgaggagcug ugcaagcagg acgacugcuu cuucgugcgg      300

cuggaggagu ccuuccucgu ccccgaggag aagcaguaca agccggccag caucuucccc      360

acccuggagg aggagaagga guacuaccag aaguacccca ccaucuacca ccuccgccag      420

aagcuggugg acuccacgga gaaggaggac cugcggcugg uguaccucgc ccuggcgcac      480

cuccugaagu accgcggcca cuuccuguuc gagggcgacc uggacaccga gaacaccagc      540

aucgaggagu ccuuccgggu cuuccucgag caguacagca agcaguccga ccagccccug      600

aucgugcacc agccggugcu gaccauccuc acggacaagc ugagcaagac caagaagguc      660

gaggagaucc ugaaguacua ccccaccgag aagaucaacu ccuucuucgc ccagugccuc      720

aagcugaucg uggggaacca ggccaacuuc aagcgcaucu ucgaccugga ggccgaggug      780

aagcuccagu ucagcaagga gaccuacgag gaggaccugg agucccugcu cgagaagauc      840

ggcgacgagu accuggacau cuuccugcag gcgaagaagg uccacgacgc cauccuccug      900

agcgagauca ucuccagcac ggugaagcac accaaggcca agcuguccag cgggauggug      960

gagcgguacg agcgccacaa ggccgaccuc gccaaguuca agcaguucgu caaggagaac     1020

gugccccaga aggcgaccgu guucuucaag gacaccacga agaacggcua cgccggguac     1080

aucaagggca agaccaccca ggaggaguuc uacaaguucg ucaagaagga gcuguccggc     1140

guggugggga gcgagcccuu ccuggagaag aucgaccagg agaccuuccu ccugaagcag     1200

cggacguaca ccaacggcgu caucccgcac caggugcacc ugaucgagcu caaggccauc     1260

aucgaccagc agaagcagca cuaccccuuc cuggaggagg ccgggcccaa gaucaucgcc     1320

cuguucaagu uccgcauccc cuacuacgug ggcccgcucg cgaaggagca ggaggccagc     1380

uccuucgccu ggaucgagcg gaagaccgcc gagaagauca accccuggaa cuucagcgag     1440

gucguggaca ucgagaaguc cgccaugcgc uucauccagc gcaugaccaa gcaggacacg     1500

uaccugccca ccgagaaggu ccugcccaag aacagccugc ucuaccagaa guacaugauc     1560

uucaacgagc ugaccaaggu guccuacaag gacgagcggg gcgugaagca guacuucagc     1620

ggggacgaga agcagcagau cuucaagcag cuguuccaga aggagcgcgg caagaucacc     1680

gucaagaagc uccagaacuu ccuguacacg cacuaccaca ucgagaacgc gcagaucuuc     1740

gggaucgaga aggccuucaa cgccuccuac agcaccuacc acgacuucau gaagcuggcc     1800

aagaccaacc agaaggccau gcaggagugg cucgagcagc cggagaugga gcccaucuuc     1860

gaggacaucg ugaagauccu gaccaucuuc gaggaccggc agaugaucaa gcaccagcug     1920

uccaaguacc aggagguguu cggcgagaag cuccugaagg aguucgcgcg caagcacuac     1980

acgggguggg gccgguucag cgccaagcug auccacggca uccgcgaccg gaagaccaac     2040

aagaccaucc ucgacuaccu gaucaacgac gacgacgucc ccgccaaccg caaccggaac     2100

cugaugcagc ucaucaacga cgagcaccug uccuucaagg aggagaucgc caaggcgacc     2160

guguucagca agcacaaguc ccugguggac gucauccagg accuccccgg gagcccggcc     2220

aucaagaagg gcaucuggca gucccugaag aucguggagg agcugaucgc caucaucggg     2280

uacaagccca agaacaucgu gaucgagaug gcccgcgaga accagaagac ccaccggacg     2340

agcccccgcc ucaaggcccu ggagaacggc cugaagcaga ucggguccac ccuccugaag     2400

gagcagccca ccgacaacaa ggcgcugcag aaggagcggc ucuaccugua cuaccugcag     2460

aacggccgcg acauguacac cggcgagccg cucgagaucg agaaccugca ccaguacgag     2520

gucgaccaca ucaucccccg gagcuucauc guggacaacu ccaucgacaa caaggugcug     2580

gucgccagca agcagaacca gaagaagcgc gacgacgugc ccaagaagca gaucgugaac     2640

gagcagcgga ucuucuggaa ccagcucaag gaggccaagc ugaucagccc caagaaguac     2700

gccuaccuga cgaagaucga gcucaccccg gaggacaagg cccgcuucau ccagcggcag     2760

cuggucgaga cccgccagau caccaagcac guggcgaaca uccugcacca guccuucaac     2820

caggaggagg aggggacgga cugcgacggc gugcagauca ucacccucaa ggccacccug     2880

accagccagu uccgccagac guucgggcug uacaaggucc gggagaucaa cccccaccac     2940

cacgcccacg acgccuaccu caacggcuuc aucgccaacg ugcugcugaa gcgcuacccc     3000

aagcucgcgc cggaguucgu guacgggaag uacgucaagu acucccuggc ccgggagaac     3060

aaggccaccg ccaagaagga guucuacagc aacauccuga aguuccucga guccgacgag     3120

cccuucugcg acgagaacgg cgagaucuac ugggagaagu cccaccaccu cccccgcauc     3180

aagaaggugc ugagcagcca ccaggugaac gucgugaaga agguggagca gcagaagggc     3240

ggguucuaca aggagaccgu caacuccaag gagaagcccg acaagcugau cgagcggaag     3300

aacaacuggg acgugaccaa guacggcggg uucggcagcc cggugaucgc guacgccauc     3360

gccuucgucu acgccaaggg caagacgcag aagaagaccc gcgccaucga ggggaucacc     3420

aucauggagc aggcggccuu cgagaaggac cccaccacgu uccugaagga gaagggcuuc     3480

ccccagguga ccgaguucau caagcugccc aaguacaccc ucuucgaguu cgacaacggg     3540

cggcgccggu uccuggccuc ccacaaggag agccagaagg gcaacccguu cauccugucc     3600

gaccagcucg ugacccugcu guaccacgcc cagcacuacg acaagaucac guaccaggag     3660

agcuucgacu acgucaacac ccaccucucc gacuucagcg ccauccugac cgaggugcug     3720

gcguucgccg agaaguacac ccucgccgac aagaacaucg agcgcaucca ggagcuguac     3780

gaggagaaca aguacgggga gacguccaug aucgcccaga gcuuccugca gcuccugcag     3840

uucaacgcca ucggcgcgcc cgccgacuuc aaguucuucg gcgugaccau cccccggaag     3900

cgcuacacca gccugaccga gaucugggac gccacgauca ucuaccaguc cgucaccggg     3960

cucuacgaga cccggauccg caugggcgac cugugggccg gggagcagug a              4011


<210>  1807
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1807
augaagaaca ugaagaagcc cuacaccauc ggccuggaca ucgggaccaa cagcgugggc       60

ugggccgucc ucacggacca guacgaccug gugaagcgca agaugaagau cgccggggac      120

uccgagaaga agcagaucaa gaagaacuuc uggggcgugc ggcuguucga cgaggggcag      180

accgcggccg accgccggau ggcccgcacc gcccggcgcc ggaucgagcg ccggcgcaac      240

cggaucagcu accuccaggg caucuucgcc gaggagaugu ccaagaccga cgcgaacuuc      300

uucugccgcc ugagcgacuc cuucuacguc gacaacgaga agcggaacag ccgccacccg      360

uucuucgcca cgaucgagga ggagguggag uaccacaaga acuaccccac caucuaccac      420

cuccgggagg agcuggugaa cuccagcgag aaggccgacc ugcgccuggu cuaccucgcc      480

cuggcgcaca ucaucaagua ccggggcaac uuccugaucg agggggcccu cgacacccag      540

aacaccuccg uggacggcau cuacaagcag uucauccaga cguacaacca gguguucgcc      600

agcgggaucg aggacggcuc ccugaagaag cuggaggaca acaaggacgu cgccaagauc      660

cucguggaga aggugacccg caaggagaag cuggagcgga uccugaagcu cuaccccggg      720

gagaaguccg ccggcauguu cgcgcaguuc aucagccuga ucgucggcag caaggggaac      780

uuccagaagc ccuucgaccu gaucgagaag uccgacaucg agugcgccaa ggacagcuac      840

gaggaggacc ucgagucccu gcuggcccuc aucggcgacg aguacgccga gcuguucgug      900

gccgcgaaga acgccuacag cgccgugguc cuguccagca ucaucaccgu ggccgagacc      960

gagacgaacg ccaagcucag cgcguccaug aucgagcgcu ucgacaccca cgaggaggac     1020

cugggggagc ugaaggccuu caucaagcuc caccucccga agcacuacga ggagaucuuc     1080

agcaacaccg agaagcacgg cuacgccggc uacaucgacg ggaagaccaa gcaggccgac     1140

uucuacaagu acaugaagau gacgcuggag aacaucgagg gcgccgacua cuucaucgcg     1200

aagaucgaga aggagaacuu ccugcggaag cagcgcaccu ucgacaacgg ggccaucccc     1260

caccagcugc accuggagga gcucgaggcc auccugcacc agcaggccaa guacuacccc     1320

uuccugaagg agaacuacga caagaucaag ucccucguga ccuuccgcau ccccuacuuc     1380

gucggcccgc uggcgaacgg gcagagcgag uucgccuggc ugacccggaa ggccgacggc     1440

gagauccgcc ccuggaacau cgaggagaag guggacuucg gcaaguccgc cguggacuuc     1500

aucgagaaga ugacgaacaa ggacaccuac cuccccaagg agaacguccu gcccaagcac     1560

agccugugcu accagaagua ccucguguac aacgagcuga ccaaggugcg guacaucaac     1620

gaccagggga agaccuccua cuucagcggc caggagaagg agcagaucuu caacgaccug     1680

uucaagcaga agcgcaaggu caagaagaag gaccucgagc uguuccugcg gaacaugucc     1740

cacguggaga gcccgacgau cgaggggcug gaggacuccu ucaacagcuc cuacagcacc     1800

uaccacgacc uccugaaggu cggcaucaag caggagaucc uggacaaccc cgugaacacc     1860

gagaugcucg agaacaucgu gaagauccug accgucuucg aggacaagcg caugaucaag     1920

gagcagcugc agcaguucuc cgacgugcuc gacggggugg uccugaagaa gcuggagcgg     1980

cgccacuaca cgggcugggg ccggcucagc gccaagcugc ugauggggau ccgcgacaag     2040

cagucccacc ucaccauccu ggacuaccug augaacgacg acggccucaa ccggaaccug     2100

augcagcuga ucaacgacag caaccucucc uucaagagca ucaucgagaa ggagcagguc     2160

accaccgcgg acaaggacau ccagagcauc guggccgacc uggccggguc ccccgccauc     2220

aagaagggca uccugcagag ccucaagauc guggacgagc ugguguccgu gaugggguac     2280

ccgccccaga cgaucgucgu ggagauggcc cgcgagaacc agaccaccgg caagggcaag     2340

aacaacagcc ggccccgcua caagucccug gagaaggcga ucaaggaguu cgggagccag     2400

auccucaagg agcaccccac cgacaaccag gagcugcgga acaaccgccu guaccucuac     2460

uaccugcaga acggcaagga cauguacacg gggcaggacc uggacaucca caaccucucc     2520

aacuacgaca ucgaccacau cgugccccag agcuucauca ccgacaacuc caucgacaac     2580

cugguccuga ccagcuccgc cggcaaccgg gagaagggcg acgacgugcc gccccucgag     2640

aucgugcgca agcggaaggu cuucugggag aagcuguacc aggggaaccu gaugagcaag     2700

cgcaaguucg acuaccucac caaggccgag cgcggcgggc ugaccgaggc cgacaaggcc     2760

cgguucaucc accgccagcu gguggagacg cggcagauca ccaagaacgu ggcgaacauc     2820

cuccaccagc gcuucaacua cgagaaggac gaccacggca acaccaugaa gcagguccgg     2880

aucgugaccc ugaaguccgc ccuggugagc caguuccgca agcaguucca gcucuacaag     2940

guccgggacg ugaacgacua ccaccacgcc cacgacgccu accugaacgg gguggucgcg     3000

aacacgcugc ucaaggugua cccccagcug gagcccgagu ucguguacgg cgacuaccac     3060

caguucgacu gguucaaggc caacaaggcc accgccaaga agcaguucua caccaacauc     3120

augcuguucu ucgcccagaa ggaccgcauc aucgacgaga acggcgagau ccucugggac     3180

aagaaguacc uggacaccgu caagaaggug auguccuacc ggcagaugaa caucgugaag     3240

aagacggaga uccagaaggg ggaguucagc aaggcgacca ucaagccgaa gggcaacucc     3300

agcaagcuga ucagccgcaa gaccaacugg gaccccauga aguacggggg ccucgacucc     3360

cccaacaugg ccuacgccgu cgugaucgag uacgccaagg ggaagaacaa gcugguguuc     3420

gagaagaaga ucauccgggu caccaucaug gagcgcaagg ccuucgagaa ggacgagaag     3480

gcguuccugg aggagcaggg cuaccggcag cccaaggugc ucgccaagcu gccgaaguac     3540

acgcuguacg agugcgagga gggccgccgg cgcaugcucg ccagcgccaa cgaggcccag     3600

aaggggaacc agcagguccu gcccaaccac cuggugaccc uccugcacca cguggcgaac     3660

ugcgaggugu ccgacggcaa gagccuggac uacaucgagu ccaaccggga gauguucgcc     3720

gagcuccugg cccacgucag cgaguucgcc aagcgcuaca cccuggcgga ggccaaccuc     3780

aacaagauca accagcuguu cgagcagaac aaggaggggg acaucaaggc caucgcccag     3840

uccuucgugg accugauggc cuucaacgcg augggcgccc ccgccagcuu caaguucuuc     3900

gagaccacga ucgagcggaa gcgcuacaac aaccucaagg agcugcugaa cuccaccauc     3960

aucuaccaga gcaucaccgg gcucuacgag ucccggaagc gccuggacga cuga           4014


<210>  1808
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1808
augaagaagu ucgagaacua cuaccugggc cucgacaucg ggaccagcuc caucggcugg       60

gccgugacca acagccagua cgacauccug aaguucaacg ggaaguacau guggggcacg      120

cgccuguucc ccgaggccaa caccgcgcag gagcggcgca uccaccgguc cagccgccgg      180

cgccucaagc ggcgcaagga gcggauccag auccugcaga ugcuguucga caaggagauc      240

gccaagaucg acuccggguu cuuccagcgc cucaaggaca gcaaguacua caaggaggac      300

aagaccgaga agcagaccaa cuccaucuuc cacgacaagg acuacagcga caaggaguac      360

caccaggacu ucccgacgau cuaccaccuc cggaaguucc ugcuggaggg caacaagccc      420

aaggacaucc gcuucgucua ccuggcccug caccacaucc ucacccaccg gggccacuuc      480

cuguuccccg acauggaggu guccaacgug accgaguuca gcaacaucuu cuccgagcug      540

aagcaguacc ucuacgacga gauggaccug gacuucgagu ggaagaacgg gaagcugaac      600

gaggucgaga acauccucaa ggaccgcaac cuggccaaga gcgagaagga gaagaagcug      660

ugcggcauca ucgagcucga ggggaagauc gacaaccagc ggaaggccau gaucggcaug      720

augugcgggu gcaagaagaa guucuccgac cuguuccaga acgagaacua ccgcgagcug      780

gagaucagcu ccuucagcuu cgcggacucc agcuacgagg agaagcggga cgagcucgag      840

ggcauccugg ccgagaacau ccugugccuc gacuaccuga agaccaucua cgacuggggc      900

aagcuguccg acauccucca gggggaggag agcaucagcg uggccaaggu gaaguccuac      960

gaggagcacc agcuggaccu gaaguaccuc aagaacaucc ugaagcugua cagccacaag     1020

gagaaggaga acgucuuccg caagaaggag ggcaaguacc ccaaguacau cuccgggaag     1080

cucagccagg aggaguucaa caaguccauc aagaagaucc uggaggagau caagagcgug     1140

aagcaggagg accggaaggu guucgacacg cugcucaagc gcgccuccaa caaccugcug     1200

ugcccgaagc aggucaucaa ggagaacggc gugauccccu accagaucca caaguucgag     1260

cucgagaaga uccugcggaa cauggcggac uucuucccca ugcugaagga gaagaaggac     1320

ggcaagaccc ucagcgagaa gaucaucucc guguucaccu uccgcauccc cuacuacguc     1380

gggccgcuga accagaacag cgaccgcgcc uggcugguga agaacaagga cgagaagauc     1440

uaccccugga acuucgagga gaucgugaac cucgaggagu ccgccgagaa guucauccag     1500

aaccugacca acaagugcac guaccugguc cuggaggacg ugcuccccaa gagcuccauc     1560

cuguacagca aguucauggu gcugaacgag cucaacaacc ugaagaucga cggcgaggcc     1620

aucuccgucg accugaagca gaagaucuac cucaaccugu uccagaagua caagaaggug     1680

acccugaaga agcucaaggg guaccucaag agcgagaaca uccugaucga caccuccacc     1740

cagaucacgg gcaucgacgg ggacuucaag agcucccugg gcagcuaccu ggacuucuac     1800

aacauccugg gcgacaaggu gaagaccgac uucgggaaga agcucaucga gaacugcauc     1860

cuguggauca cccuguacac cggcgagaag aagcuccuga agaacaagau caucgccaac     1920

uacaaggggg agcugagcga ggaggagauc aagaagaucg ucaaccucaa guacaaggac     1980

uggggccggc uguccuacgc guuccuggag gagauccaga gcgccucccu cgagacgggg     2040

gagcugcgca acaucaucca gaugaugugg gagaccaaca acaaccugau ggagcuccug     2100

agcuccaacu accaguuccu gagcgagauc gagaagcgga acuccguggu ggccaucggc     2160

aaggaguuca acuacgagac cauccucggc gacagcuacg ccucccccag cgucaagcgc     2220

augaucuggc agucccugag cguggucgac gagaucaaga agaucaugaa gaaggccccg     2280

aagaagaucu ucaucgagau ggcgcggcag gaggacauga agaaggagcg caaggagucc     2340

cgcaagagca ccuuccugac gcucuacaag uccaucaagg aggaggggcg ggacuggauc     2400

aaggagaucg agaacuggag cgacuccgag uuccggagca agaagcugua ccuguacuac     2460

acccagaugg gcaagugcau guacaccggg gagaagauca gccucgacca gcuguucaac     2520

aagaacaucu acgacaucga ccacaucuac ccccgcucca agaccaagga cgacagcauc     2580

gagaacaucg ugcuggugaa gcggaacauc aacgccaaga agacggacga guacccccuc     2640

gagcgcaaca uccagcagaa gcagcacgac uucuggaaga ugcugcacuc caagaagcug     2700

aucggcgaca agaaguacga gcggcucacc cgcaccaccg aguucacgga cgaggagcug     2760

agcgacuuca ucgcccggca gcuggucgag acccgccagu ccaccaagau cguggccgac     2820

auccucaaga accuguuccc cgagaccaag aucguguacg ucaaggccaa ccugacgagc     2880

gacuuccgga agaacuucaa gauccucaag ucccgcgaca ucaacgacua ccaccacgcg     2940

cacgacgccu accugaacau cgugaccggg aacguguaca acaucaaguu caccgacaac     3000

ccgcggaacu ucaucaagga caagaagcug gagggcaaga aguacaaccu caaggucgag     3060

aagaucuucg aguaccgcaa ggagaacgag agcuucugga accccaagac cauggugacc     3120

aagaucgaga acuacaucuu caagaagcgc ccccaguuca cgcgguacuc cuucgagcag     3180

cacggcgggu ucuucgacca gaacaucgug gacaaggaga gcugcaagaa gggcaccggg     3240

uacaucccca ucaaguccag caacgccauc cuguacaaca ccuccaagua cggcggcuac     3300

gggagcguca ccggcacgua cuucuuccug guggagcaca ccaugaaggg gaagcgcauc     3360

cggaccaucg agaugaugcc gcucuaccug uccaagaaga uccacagcaa ggaggagcug     3420

gagaaguacu gcaaggagaa gcucgagcug cagaagcccu ccgugcgccu ggcccggauc     3480

aaguacaaca gccuccugaa gaucaacggc uuccccuacc acaucaccgg gaagacgaac     3540

gacuccuacu ggaucaugag cgccauccag cugcuccuga gcaagaacua cuacgaguac     3600

cugcgcaagc ucuacaucuu cugcaaggag gagcggcugg aggaggagau cgucggcgag     3660

aagaacauca agcuguacac gugcauccuc gagaagcugg agaaguccau cuacagccac     3720

aagcugcuca acaucaacua caacggcaag uccaagaacc ugaaggacau ccuggagagc     3780

gagaaggaga acuuccuccu gcuguccgag aagaagcagg cgcagauccu caucgagauc     3840

uucacccugc uggagagcaa caacuucggg gccaaccucg aguccuucgg cugcgggaag     3900

aagugcggca ucaccaagau caacaagaac aucgacaagc uggaggaggu gcugcucauc     3960

aaccagagcc ccaccgggcu guucgagaac uccaucgacc ugaagaaggu guga           4014


<210>  1809
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1809
augaagaagg acuacgugau cggccuggac aucgggacca acagcgucgg cugggccgug       60

augaccgagg acuaccagcu cgugaagaag aagaugccca ucuacgggaa cacggagaag      120

aagaagauca agaagaacuu cuggggcguc cgccuguucg aggaggggca caccgccgag      180

gaccggcgcc ugaagcggac cgcgcgccgg cgcaucuccc ggcgccggaa ccgccuccgg      240

uaccugcagg ccuucuucga ggaggccaug accgaccugg acgagaacuu cuucgcccgc      300

cuccaggaga gcuuccuggu gccggaggac aagaaguggc accggcaccc caucuucgcc      360

aagcuggagg acgagguggc guaccacgag acguacccca ccaucuacca ccuccgcaag      420

aagcuggccg acuccagcga gcaggccgac cugcggcuca ucuaccuggc ccuggcgcac      480

aucgucaagu accgcggcca cuuccucauc gagggcaagc uguccaccga gaacaucagc      540

gugaaggagc aguuccagca guucaugauc aucuacaacc agaccuucgu gaacggggag      600

ucccggcugg ucagcgcgcc ccucccggag uccgugcuga ucgaggagga gcugacggag      660

aaggccagcc gcaccaagaa guccgagaag gugcuccagc aguuccccca ggagaaggcc      720

aacggccugu ucgggcaguu ccugaagcuc auggucggca acaaggccga cuucaagaag      780

guguucgggc uggaggagga ggccaagauc accuacgcca gcgaguccua cgaggaggac      840

cuggagggca uccucgccaa ggugggcgac gaguacagcg acgucuuccu ggccgccaag      900

aacguguacg acgcggugga gcuguccacc auccucgccg acagcgacaa gaagucccac      960

gccaagcuga gcuccagcau gaucguccgg uucacggagc accaggagga ccugaagaag     1020

uucaagcgcu ucauccggga gaacugcccc gacgaguacg acaaccucuu caagaacgag     1080

cagaaggacg gguacgccgg cuacaucgcc cacgcgggga aggugagcca gcugaaguuc     1140

uaccaguacg ugaagaagau cauccaggac aucgccggcg ccgaguacuu ccuggagaag     1200

aucgcccagg agaacuuccu ccgcaagcag cgcaccuucg acaacggcgu caucccccac     1260

cagauccacc uggccgagcu gcaggcgauc auccaccggc aggccgccua cuacccguuc     1320

cucaaggaga accaggagaa gaucgagcag cuggugaccu uccgcauccc cuacuacgug     1380

gggccccugu ccaagggcga cgccagcacc uucgcguggc ucaagcggca guccgaggag     1440

cccauccgcc cguggaaccu gcaggagacg gucgaccugg accagagcgc caccgccuuc     1500

aucgagcgga ugaccaacuu cgacaccuac cuccccuccg agaaggugcu gcccaagcac     1560

agccugcucu acgagaaguu cauggucuuc aacgagcuga cgaagaucuc cuacaccgac     1620

gaccgcggga ucaaggccaa cuucagcggc aaggagaagg agaagaucuu cgacuaccug     1680

uucaagaccc ggcgcaaggu gaagaagaag gacaucaucc aguucuaccg gaacgaguac     1740

aacaccgaga ucgugacgcu guccgggcuc gaggaggacc aguucaacgc cagcuucucc     1800

accuaccagg accugcugaa gugcggccuc acccgcgcgg agcuggacca ccccgacaac     1860

gccgagaagc uggaggacau caucaagauc cucaccaucu ucgaggaccg gcagcgcauc     1920

cggacgcagc ugagcaccuu caagggccag uucuccgccg agguccugaa gaagcucgag     1980

cgcaagcacu acaccgggug gggccggcug agcaagaagc ugaucaacgg gaucuacgac     2040

aaggaguccg gcaagaccau ccucggguac cugaucaagg acgacggcgu gagcaagcac     2100

uacaaccgca acuucaugca gcugaucaac gacucccagc ucagcuucaa gaacgccauc     2160

cagaaggccc aguccagcga gcacgaggag acgcugagcg agaccgugaa cgagcuggcg     2220

ggcuccccgg ccaucaagaa ggggaucuac cagagccuca agaucgucga cgagcuggug     2280

gccaucaugg gcuacgcccc caagcggauc guggucgaga uggcccgcga gaaccagacc     2340

accuccacgg ggaagcggcg cagcauccag cgccugaaga ucguggagaa ggcgauggcc     2400

gagaucggcu ccaaccuccu gaaggagcag cccaccacca acgagcagcu gcgggacacc     2460

cgccucuucc uguacuacau gcagaacggg aaggacaugu acaccggcga cgagcugagc     2520

cuccaccggc ugucccacua cgacaucgac cacaucaucc cccagagcuu caugaaggac     2580

gacucccugg acaaccucgu gcuggucggc agcacggaga accgcgggaa guccgacgac     2640

gugccgagca aggagguggu caaggacaug aaggccuacu gggagaagcu guacgccgcg     2700

ggccucaucu cccagcggaa guuccagcgc cugaccaagg gggagcaggg cggccugacc     2760

cucgaggaca aggcccacuu cauccagcgg cagcuggugg agacccgcca gaucacgaag     2820

aacguggccg ggauccugga ccagcgguac aacgccaaca gcaaggagaa gaagguccag     2880

aucaucaccc ucaaggccuc ccugaccagc caguuccgcu ccaucuucgg ccuguacaag     2940

gugcgggagg ugaacgacua ccaccacggg caggacgcgu accucaacug cgucguggcc     3000

accacgcugc ugaaggugua ccccaaccuc gcccccgagu ucgucuacgg cgaguacccc     3060

aaguuccaga ccuucaagga gaacaaggcc accgccaagg cgaucaucua caccaaccug     3120

cugcgcuucu ucacggagga cgagccgcgg uucaccaagg acggggagau ccucuggagc     3180

aacagcuacc ugaagaccau caagaaggag cugaacuacc accagaugaa caucgugaag     3240

aagguggagg uccagaaggg cggcuucucc aaggagagca ucaagcccaa ggggcccucc     3300

aacaagcuca uccccgugaa gaacggccug gacccgcaga aguacggggg cuucgacagc     3360

cccaucgugg ccuacaccgu ccuguucacg cacgagaagg ggaagaagcc ccucaucaag     3420

caggagaucc ugggcaucac caucauggag aagacccgcu ucgagcagaa cccgauccug     3480

uuccucgagg agaagggcuu ccugcggccc cgcgugcuga ugaagcuccc caaguacacc     3540

cuguacgagu uccccgaggg gcggcgccgg cugcucgccu ccgccaagga ggcccagaag     3600

ggcaaccaga uggugcugcc ggagcaccuc cugacgcugc uguaccacgc gaagcagugc     3660

cuccugccca accagagcga gucccugacc uacgucgagc agcaccagcc cgaguuccag     3720

gagauccucg agcgcguggu ggacuucgcc gagguccaca cccuggccaa gagcaaggug     3780

cagcagaucg ugaagcuguu cgaggccaac cagaccgcgg acgucaagga gaucgccgcc     3840

uccuucaucc agcucaugca guucaacgcc augggggccc ccagcacguu caaguucuuc     3900

cagaaggaca ucgagcgggc gcgcuacacc uccaucaagg agaucuucga cgccaccauc     3960

aucuaccaga gcaccacggg ccuguacgag acccgccgga agguggugga cuga           4014


<210>  1810
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1810
augaagaagg acuacgugau cggccuggac aucgggacca acagcgucgg cugggccgug       60

augaccgagg acuaccagcu cgugaagaag aagaugccca ucuacgggaa cacggagaag      120

aagaagauca agaagaacuu cuggggcguc cgccuguucg aggaggggca caccgccgag      180

gaccggcgcc ugaagcggac cgcgcgccgg cgcaucuccc ggcgccggaa ccgccuccgg      240

uaccugcagg ccuucuucga ggaggccaug accgaccugg acgagaacuu cuucgcccgc      300

cuccaggaga gcuuccuggu gccggaggac aagaaguggc accggcaccc caucuucgcc      360

aagcuggagg acgagguggc guaccacgag acguacccca ccaucuacca ccuccgcaag      420

aagcuggccg acuccagcga gcaggccgac cugcggcuca ucuaccuggc ccuggcgcac      480

aucgucaagu accgcggcca cuuccucauc gagggcaagc uguccaccga gaacaccagc      540

gugaaggacc aguuccagca guucauggug aucuacaacc agacguucgu caacggggag      600

ucccggcugg ugagcgcgcc ccucccggag uccgugcuga ucgaggagga gcugaccgag      660

aaggccagcc gcaccaagaa guccgagaag guccuccagc aguuccccca ggagaaggcc      720

aacggccugu ucgggcaguu ccugaagcuc auggugggca acaaggccga cuucaagaag      780

guguucgggc uggaggagga ggccaagauc accuacgcca gcgaguccua cgaggaggac      840

cuggagggca uccucgccaa ggucggcgac gaguacagcg acguguuccu ggccgccaag      900

aacguguacg acgcggucga gcuguccacg auccucgccg acagcgacaa gaagucccac      960

gccaagcuga gcuccagcau gaucgugcgg uucaccgagc accaggagga ccugaagaag     1020

uucaagcgcu ucauccggga gaacugcccc gacgaguacg acaaccucuu caagaacgag     1080

cagaaggacg gguacgccgg cuacaucgcc cacgcgggga aggugagcca gcugaaguuc     1140

uaccaguacg ucaagaagau cauccaggac aucgccggcg ccgaguacuu ccuggagaag     1200

aucgcccagg agaacuuccu ccgcaagcag cgcaccuucg acaacggcgu gaucccccac     1260

cagauccacc uggccgagcu gcaggcgauc auccaccggc aggccgccua cuacccguuc     1320

cucaaggaga accaggagaa gaucgagcag cuggugaccu uccgcauccc cuacuacguc     1380

gggccccugu ccaagggcga cgccagcacg uucgcguggc ucaagcggca guccgaggag     1440

cccauccgcc cguggaaccu gcaggagacc guggaccugg accagagcgc caccgccuuc     1500

aucgagcgga ugaccaacuu cgacacguac cuccccuccg agaagguccu gcccaagcac     1560

agccugcucu acgagaaguu caugguguuc aacgagcuga ccaagaucuc cuacaccgac     1620

gaccgcggga ucaaggccaa cuucagcggc aaggagaagg agaagaucuu cgacuaccug     1680

uucaagaccc ggcgcaaggu gaagaagaag gacaucaucc aguucuaccg gaacgaguac     1740

aacacggaga ucgucacccu guccgggcuc gaggaggacc aguucaacgc cagcuucucc     1800

accuaccagg accugcugaa gugcggccuc acccgcgcgg agcuggacca ccccgacaac     1860

gccgagaagc uggaggacau caucaagauc cucacgaucu ucgaggaccg gcagcgcauc     1920

cggacccagc ugagcaccuu caagggccag uucuccgccg aggugcugaa gaagcucgag     1980

cgcaagcacu acaccgggug gggccggcug agcaagaagc ugaucaacgg gaucuacgac     2040

aaggaguccg gcaagacgau ccucgacuac cugaucaagg acgacggggu gagcaagcac     2100

uacaaccgca acuucaugca gcugaucaac gacucccagc ucagcuucaa gaacgccauc     2160

cagaaggccc aguccugcga gcacgaggag acccugagcg agaccgucaa cgagcuggcg     2220

ggcagcccgg ccaucaagaa gggcaucuac cagucccuca agaucgugga cgagcuggug     2280

gccaucaugg gguacgcccc caagcggauc gucguggaga uggcccgcga gaaccagacc     2340

acgagcaccg gcaagcggcg cuccauccag cgccugaaga ucguggagaa ggcgauggcc     2400

gagaucggga gcaaccuccu gaaggagcag cccaccacca acgagcagcu gcgggacacc     2460

cgccucuucc uguacuacau gcagaacggc aaggacaugu acacggggga cgagcugucc     2520

cuccaccggc ugagccacua cgacaucgac cacaucaucc cccaguccuu caugaaggac     2580

gacagccugg acaaccucgu ccuggugggc uccaccgaga accgcggcaa gagcgacgac     2640

gugccgucca agaaggucgu gaaggacaug aaggccuacu gggagaagcu guacgccgcg     2700

gggcucauca gccagcggaa guuccagcgc cugaccaagg gcgagcaggg cgggcugacc     2760

cucgaggaca aggcccacuu cauccagcgg cagcuggugg agacgcgcca gaucaccaag     2820

aacgucgccg gcauccugga ccagcgguac aacgccaagu ccaaggagaa gaaggugcag     2880

aucaucaccc ucaaggccag ccugaccucc caguuccgca gcaucuucgg gcuguacaag     2940

gugcgggagg ucaacgacua ccaccacggc caggacgcgu accucaacug cgugguggcc     3000

acgacccugc ugaaggucua ccccaaccuc gcccccgagu ucguguacgg ggaguacccc     3060

aaguuccagg ccuucaagga gaacaaggcc accgcgaaga ccaucaucua cacgaaccug     3120

augcgcuucu ucaccgagga cgagccgcgg uucaccaagg acuccgagau ccuguggagc     3180

aacagcuacc ucaagaccau caagaaggag cugaacuacc accagaugaa caucgugaag     3240

aaggucgagg ugcagaaggg cggguucucc aaggagagca ucaagcccaa gggccccucc     3300

aacaagcuga uccccgugaa gaacggccuc gacccgcaga aguacggggg cuucgacagc     3360

cccgucgugg ccuacacggu gcuguucacc cacgagaagg ggaagaagcc ccugaucaag     3420

caggagaucc ucggcaucac caucauggag aagacccgcu ucgagcagaa cccgauccug     3480

uuccuggagg agaagggguu ccuccggccc cgcguccuga ugaagcugcc caaguacacg     3540

cucuacgagu uccccgaggg ccggcgccgg cugcuggccu ccgccaagga ggcccagaag     3600

ggcaaccaga uggugcuccc ggagcaccuc cugacccugc uguaccacgc gaagcagugc     3660

cugcucccca accagagcga gucccuggcc uacguggagc agcaccagcc cgaguuccag     3720

gagauccugg agcgcgucgu ggacuucgcc gaggugcaca cccucgccaa gagcaagguc     3780

cagcagaucg ugaagcuguu cgaggcgaac cagaccgccg acgugaagga gaucgccgcc     3840

uccuucaucc agcugaugca guucaacgcc augggggcgc ccagcacguu caaguucuuc     3900

cagaaggaca ucgagcgggc ccgcuacacc uccaucaagg agaucuucga cgccaccauc     3960

aucuaccaga gcaccacggg ccucuacgag acccgccgga aggucgugga cuga           4014


<210>  1811
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1811
augaagaagg acuacgugau cggccuggac aucgggacca acagcgucgg cugggccgug       60

augaccgagg acuaccagcu cgugaagaag aagaugccca ucuacgggaa cacggagaag      120

aagaagauca agaagaacuu cuggggcguc cgccuguucg aggaggggca caccgccgag      180

gaccggcgcc ugaagcggac cgcgcgccgg cgcaucuccc ggcgccggaa ccgccuccgg      240

uaccugcagg ccuucuucga ggaggccaug accgcccugg acgagaacuu cuucgcccgc      300

cuccaggaga gcuuccuggu gccggaggac aagaaguggc accggcaccc caucuucgcg      360

aagcuggagg acgagguggc cuaccacgag acguacccca ccaucuacca ccuccgcaag      420

aagcuggccg acuccagcga gcaggccgac cugcggcuca ucuaccuggc gcuggcccac      480

aucgucaagu accgcggcca cuuccucauc gagggcaagc uguccaccga gaacaucagc      540

gugaaggagc aguuccagca guucaugauc aucuacaacc agaccuucgu gaacggggag      600

ucccggcugg ucagcgcgcc ccucccggag uccgugcuga ucgaggagga gcugacggag      660

aaggccagcc gcaccaagaa guccgagaag gugcuccagc aguuccccca ggagaaggcc      720

aacggccugu ucgggcaguu ccugaagcuc auggucggca acaaggccga cuucaagaag      780

guguucgggc uggaggagga ggccaagauc accuacgcca gcgaguccua cgaggaggac      840

cuggagggca uccucgccaa ggugggcgac gaguacagcg acgucuuccu ggccgcgaag      900

aacguguacg acgccgugga gcuguccacc auccucgccg acagcgacaa gaagucccac      960

gccaagcuga gcuccagcau gaucguccgg uucacggagc accaggagga ccugaagaac     1020

uucaagcgcu ucauccggga gaacugcccc gacgaguacg acaaccucuu caagaacgag     1080

cagaaggacg gguacgccgg cuacaucacc cacgcgggga aggugagcca gcugaaguuc     1140

uaccaguacg ugaagaagau cauccaggac aucgccggcg ccgaguacuu ccuggagaag     1200

aucgcccagg agaacuuccu ccgcaagcag cgcaccuucg acaacggcgu caucccccac     1260

cagauccacc uggccgagcu gcaggcgauc auccaccggc aggccgccua cuacccguuc     1320

cucaaggaga accaggagaa gaucgagcag cuggugaccu uccgcauccc cuacuacgug     1380

gggccccugu ccaagggcga cgccaacacg uucgcguggc ucaagcggca gagcgaggag     1440

cccauccgcc cguggaaccu gcaggagacc gucgaccugg accaguccgc caccgccuuc     1500

aucgagcgga ugaccaacuu cgacacguac cuccccagcg agaaggugcu gcccaagcac     1560

ucccugcucu acgagaaguu cauggucuuc aacgagcuga ccaagaucag cuacaccgac     1620

gaccgcggga ucaaggccaa cuucuccggc aaggagaagg agaagaucuu cgacuaccug     1680

uucaagaccc ggcgcaaggu gaagaagaag gacaucaucc aguucuaccg gaacgaguac     1740

aacacggaga ucgugacccu gagcgggcuc gaggaggacc aguucaacgc cuccuucagc     1800

accuaccagg accugcugaa gugcggccuc acccgcgcgg agcuggacca ccccgacaac     1860

gccgagaagc uggaggacau caucaagauc cucacgaucu ucgaggaccg gcagcgcauc     1920

cggacccagc uguccaccuu caagggccag uucagcgccg agguccugaa gaagcucgag     1980

cgcaagcacu acaccgggug gggccggcug uccaagaagc ugaucaacgg gaucuacgac     2040

aaggagagcg gcaagacgau ccucgacuac cugaucaagg acgacggggu guccaagcac     2100

uacaaccgca acuucaugca gcugaucaac gacagccagc ucuccuucaa gaacgccauc     2160

cagaaggccc agagcuccga gcacgaggag acccugagcg agaccgugaa cgagcuggcg     2220

ggcagcccgg ccaucaagaa gggcaucuac cagucccuca agaucgucga cgagcuggug     2280

gccaucaugg gguacgcccc caagcggauc guggucgaga uggcccgcga gaaccagacc     2340

acgagcaccg gcaagcggcg cuccauccag cgccugaaga ucguggagaa ggcgauggcc     2400

gagaucggga gcaaccuccu gaaggagcag cccaccacca acgagcagcu gcgggacacc     2460

cgccucuucc uguacuacau gcagaacggc aaggacaugu acacggggga cgagcugucc     2520

cuccaccggc ugagccacua cgacaucgac cacaucaucc cccaguccuu caugaaggac     2580

gacagccugg acaaccucgu gcuggucggc uccaccgaga accgcggcaa gagcgacgac     2640

gugccgucca aggagguggu caaggacaug aaggccuacu gggagaagcu guacgccgcg     2700

gggcucauca gccagcggaa guuccagcgc cugaccaagg gcgagcaggg cgggcugacc     2760

cucgaggaca aggcccacuu cauccagcgg cagcuggugg agacgcgcca gaucaccaag     2820

aacguggccg gcauccugga ccagcgguac aacgccaagu ccaaggagaa gaagguccag     2880

aucaucaccc ucaaggccag ccugaccucc caguuccgca gcaucuucgg gcuguacaag     2940

gugcgggagg ugaacgacua ccaccacggc caggacgcgu accucaacug cgucguggcc     3000

acgacccugc ugaaggugua ccccaaccuc gcccccgagu ucgucuacgg ggaguacccc     3060

aaguuccaga ccuucaagga gaacaaggcc accgccaagg cgaucaucua cacgaaccug     3120

cugcgcuucu ucaccgagga cgagccgcgg uucaccaagg acggcgagau ccucuggucc     3180

aacagcuacc ugaagaccau caagaaggag cugaacuacc accagaugaa caucgugaag     3240

aagguggagg uccagaaggg cggguucagc aaggagucca ucaagcccaa gggccccagc     3300

aacaagcuca uccccgugaa gaacgggcug gacccgcaga aguacggcgg guucgacucc     3360

cccaucgugg ccuacacggu ccuguucacc cacgagaagg gcaagaagcc ccucaucaag     3420

caggagaucc uggggaucac caucauggag aagacccgcu ucgagcagaa cccgauccug     3480

uuccucgagg agaagggcuu ccugcggccc cgcgugcuga ugaagcuccc caaguacacg     3540

cuguaccagu uccccgaggg ccggcgccgg cugcucgcca gcgccaagga ggcccagaag     3600

gggaaccaga uggugcugcc ggagcaccuc cugacccugc uguaccacgc gaagcagugc     3660

cuccugccca accaguccga gagccuggcc uacgucgagc agcaccagcc cgaguuccag     3720

gagauccucg agcgcguggu ggacuucgcc gagguccaca cccuggccaa guccaaggug     3780

cagcagaucg ugaagcuguu cgaggcgaac cagaccgccg acgucaagga gaucgccgcc     3840

agcuucaucc agcucaugca guucaacgcc augggcgcgc ccuccacguu caaguucuuc     3900

cagaaggaca ucgagcgggc ccgcuacacc agcaucaagg agaucuucga cgccaccauc     3960

aucuaccagu ccaccacggg gcuguacgag acccgccgga agguggugga cuga           4014


<210>  1812
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1812
augaagaagg acuacgugau cggccuggac aucgggacca acagcgucgg cugggccgug       60

augaccgagg acuaccagcu cgugaagaag aagaugccca ucuacgggaa cacggagaag      120

aagaagauca agaagaacuu cuggggcguc cgccuguucg aggaggggca caccgccgag      180

gaccggcgcc ugaagcggac cgcgcgccgg cgcaucuccc ggcgccggaa ccgccuccgg      240

uaccugcagg ccuucuucga ggaggccaug accgaccugg acgagaacuu cuucgcccgc      300

cuccaggaga gcuuccuggu gccggaggac aagaaguggc accggcaccc caucuucgcc      360

aagcuggagg acgagguggc guaccacgag acguacccca ccaucuacca ccuccgcaag      420

aagcuggccg acuccagcga gcaggccgac cugcggcuca ucuaccuggc ccuggcgcac      480

aucgucaagu accgcggcca cuuccucauc gagggcaagc uguccaccga gaacaucagc      540

gugaaggagc aguuccagca guucaugauc aucuacaacc agaccuucgu gaacggggag      600

ucccggcugg ucagcgcgcc ccucccggag uccgugcuga ucgaggagga gcugacggag      660

aaggccagcc gcaccaagaa guccgagaag gugcuccagc aguuccccca ggagaaggcc      720

aacggccugu ucgggcaguu ccugaagcuc auggucggca acaaggccga cuucaagaag      780

guguucgggc uggaggagga ggccaagauc accuacgcca gcgaguccua cgaggaggac      840

cuggagggca uccucgccaa ggugggcgac gaguacagcg acgucuuccu ggccgccaag      900

aacguguacg acgcggugga gcuguccacc auccucgccg acagcgacaa gaagucccac      960

gccaagcuga gcuccagcau gaucguccgg uucacggagc accaggagga ccugaagaag     1020

uucaagcgcu ucauccggga gaacugcccc gacgaguacg acaaccucuu caagaacgag     1080

cagaaggacg gguacgccgg cuacaucgcc cacgcgggga aggugagcca gcugaaguuc     1140

uaccaguacg ugaagaagau cauccaggac aucgccggcg ccgaguacuu ccuggagaag     1200

aucgcccagg agaacuuccu ccgcaagcag cgcaccuucg acaacggcgu caucccccac     1260

cagauccacc uggccgagcu gcaggcgauc auccaccggc aggccgccua cuacccguuc     1320

cucaaggaga accaggagaa gaucgagcag cuggugaccu uccgcauccc cuacuacgug     1380

gggccccugu ccaagggcga cgccagcacc uucgcguggc ucaagcggca guccgaggag     1440

cccauccgcc cguggaaccu gcaggagacg gucgaccugg accagagcgc caccgccuuc     1500

aucgagcgga ugaccaacuu cgacaccuac cuccccuccg agaaggugcu gcccaagcac     1560

agccugcucu acgagaaguu cauggucuuc aacgagcuga cgaagaucuc cuacaccgac     1620

gaccgcggga ucaaggccaa cuucagcggc aaggagaagg agaagaucuu cgacuaccug     1680

uucaagaccc ggcgcaaggu gaagaagaag gacaucaucc aguucuaccg gaacgaguac     1740

aacaccgaga ucgugacgcu guccgggcuc gaggaggacc aguucaacgc cagcuucucc     1800

accuaccagg accugcugaa gugcggccuc acccgcgcgg agcuggacca ccccgacaac     1860

gccgagaagc uggaggacau caucaagauc cucaccaucu ucgaggaccg gcagcgcauc     1920

cggacgcagc ugagcaccuu caagggccag uucuccgccg agguccugaa gaagcucgag     1980

cgcaagcacu acaccgggug gggccggcug agcaagaagc ugaucaacgg gaucuacgac     2040

aaggaguccg gcaagaccau ccucggguac cugaucaagg acgacggcgu gagcaagcac     2100

uacaaccgca acuucaugca gcugaucaac gacucccagc ucagcuucaa gaacgccauc     2160

cagaaggccc aguccagcga gcacgaggag acgcugagcg agaccgugaa cgagcuggcg     2220

ggcuccccgg ccaucaagaa ggggaucuac cagagccuca agaucgucga cgagcuggug     2280

gccaucaugg gcuacgcccc caagcggauc guggucgaga uggcccgcga gaaccagacc     2340

accuccacgg ggaagcggcg cagcauccag cgccugaaga ucguggagaa ggcgauggcc     2400

gagaucggcu ccaaccuccu gaaggagcag cccaccacca acgagcagcu gcgggacacc     2460

cgccucuucc uguacuacau gcagaacggg aaggacaugu acaccggcga cgagcugagc     2520

cuccaccggc ugucccacua cgacaucgac cacaucaucc cccagagcuu caugaaggac     2580

gacucccugg acaaccucgu gcuggucggc agcacggaga accgcgggaa guccgacgac     2640

gugccgagca aggagguggu caaggacaug aaggccuacu gggagaagcu guacgccgcg     2700

ggccucaucu cccagcggaa guuccagcgc cugaccaagg gggagcaggg cggccugacc     2760

cucgaggaca aggcccacuu cauccagcgg cagcuggugg agacccgcca gaucacgaag     2820

aacguggccg ggauccugga ccagcgguac aacgccaaca gcaaggagaa gaagguccag     2880

aucaucaccc ucaaggccuc ccugaccagc caguuccgcu ccaucuucgg ccuguacaag     2940

gugcgggagg ugaacgacua ccaccacggg caggacgcgu accucaacug cgucguggcc     3000

accacgcugc ugaaggugua ccccaaccuc gcccccgagu ucgucuacgg cgaguacccc     3060

aaguuccaga ccuucaagga gaacaaggcc accgccaagg cgaucaucua caccaaccug     3120

cugcgcuucu ucacggagga cgagccgcgg uucaccaagg acggggagau ccucuggagc     3180

aacagcuacc ugaagaccau caagaaggag cugaacuacc accagaugaa caucgugaag     3240

aagguggagg uccagaaggg cggcuucucc aaggagagca ucaagcccaa ggggcccucc     3300

aacaagcuca uccccgugaa gaacggccug gacccgcaga aguacggggg cuucgacagc     3360

cccaucgugg ccuacaccgu ccuguucacg cacgagaagg ggaagaagcc ccucaucaag     3420

caggagaucc ugggcaucac caucauggag aagacccgcu ucgagcagaa cccgauccug     3480

uuccucgagg agaagggcuu ccugcggccc cgcgugcuga ugaagcuccc caaguacacc     3540

cuguacgagu uccccgaggg gcggcgccgg cugcucgccu ccgccaagga ggcccagaag     3600

ggcaaccaga uggugcugcc ggagcaccuc cugacgcugc uguaccacgc gaagcagugc     3660

cuccugccca accagagcga gucccuggcc uacgucgagc agcaccagcc cgaguuccag     3720

gagauccucg agcgcguggu ggacuucgcc gagguccaca cccuggccaa gagcaaggug     3780

cagcagaucg ugaagcuguu cgaggcgaac cagaccgccg acgucaagga gaucgccgcc     3840

uccuucaucc agcucaugca guucaacgcc augggggcgc ccagcaccuu caaguucuuc     3900

cagaaggaca ucgagcgggc ccgcuacacg uccaucaagg agaucuucga cgccaccauc     3960

aucuaccaga gcaccaccgg ccuguacgag acgcgccgga agguggugga cuga           4014


<210>  1813
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1813
augcaggacu acuaccuggg ccucgacauc gggaccaaca gcgugggcug ggccgucacc       60

gacgagaacu acaaccugcu gcgcaagaag gggaaggacc ucuggggcgu gcggcuguuc      120

aaggaggccc agcccgcgac ggagcgccgg gggcaccgcu ccagccggcg ccggaccauc      180

cgcaaguccc ugcggaagca gcuccugcgc gagcuguucg aggaggagau caacgccauc      240

gacaaggacu ucuuccuccg gcuggacgag agcaaguacu ggcuggagga caagaagauc      300

gagggcaagu acucccucuu caacgacgag aacuucaccg acaaggacua cuacgagaag      360

uucccgacca ucuuccaccu ccgcaaggcc cugaucgaga gcgacgacaa ggaggacauc      420

cggcucuacu uccuggccau caaccagaug augaagcgcc ggggccacuu ccugaucgac      480

gggaagcucu ccaacgugga ggacaucacg cccuucaagg agugcaucca gaaccugauc      540

aacgaggagc uggacaccga gaucgagacc gaguucuacg acaaggccau caagaucguc      600

agcgacuacu cccucaagaa gaccgagaag aagaaccuga ucaaggaccu ggugaaggag      660

acggacagca ucgaggacuc caagaagauc aucaacaucu ucaaccuccu gaucagcggc      720

aaguccaagg ugaacgggau cuucgacgac gaggagaucc uggagaagau caaggaggac      780

aacaaggagg acuucuaccu caccagcgag aaguacgagg agaaccugga guacuucucc      840

cagcugcucg acuaccgcuu cgagcugauc agcucccuca agagcaucua cgacuacauc      900

auccugcagg agguccugaa gggcaagaag uaccuguccu acgcccaggu ggagcgguac      960

aagaaccaca aggaggaccu ccgcaagcug aagcagcuga ucaagaagua cgacaagaac     1020

gggaagaucu acuaccagau cuuccgggac gagaacuuca agaagggcua cguggcguac     1080

cucggcuacu acaagaagaa caagaagaac guccccguga agaagugcga cuacgacacc     1140

uucagcaaga acaucaagag cacccuggac aagaucugcg acaaggagga cccccuguac     1200

aucgagcuca ucgacaagau cgagaagggc ggguuccugc ugaagcagau cguguccacg     1260

aacagcguca ucccgcacca ggugcacgag cucgagcugg acaagauccu ggagaaccuc     1320

gagaagaacu accccuccuu caaggagauc agcgacgggg uguccaagau cgacaagauc     1380

aagaugaucu ucaacuuccg cauccccuac uacgucggcc cccugaacga cuaccacaag     1440

gacaagguga acagcaacac cuggaucgug aagaacgagg gcuucgagaa cgagaacauc     1500

cggccgugga acuucuccaa gaucgucgac gagcacgccu gcgaggagga guucaucaag     1560

cgcaugauca acgagugcag cuacaucccc ggggagaagg ugcugcccaa guccagccug     1620

cucuacuccg aguacauggu gcugaacgag cugaacaacc uccgcgucaa cggcgacaag     1680

cugaacccca agaucaagaa ggagaucgug gagaacaucu acaagaagga gcggaaggug     1740

acccugaaga agauccgcaa cuuccucauc aucaacaacu acgucgacag caagguggag     1800

uucuccgggc ugagcgacgg caucaaggcc aacaugucca gcuacaucga cuucgcggag     1860

auccuggacg acaacuucga caacaacaug gucgaggacc ucaucgagca gaucaccauc     1920

cacgugggga acgugaagcu gcugacggag aagaucucca acaaguaccc gaaccucgag     1980

aagaagcaga ucaacaagau cgucaaccug aaguucaagg acugggcccg guucagccgc     2040

aagcugcucg ugggcaucga gggcaccgag aaggagaccg gggagaucaa caccaucauc     2100

ggcuuccugc gggagacgaa cgagaacuuc auggagcuga uguccaaccg cuacaccuuc     2160

agcgaggaga ucagcgagau caagaagaag uacaucccca acaagcucuc caagcagauc     2220

guggacgacc uguacgucag ccccgccgug aagaagauga ucuggcaggu ccugaacauc     2280

aacgacgagc ucaucaaggu gcuggggaag cagcccaaga agaucuucau cgagauggcc     2340

cggggcaagg aggagaagcc ggaggugaag gacucccgca agaacaagcu gaucgagcuc     2400

uacaagaacc uggggaagga gggcaaggag uggcugaacc ggaucgagga caagaccgag     2460

gccgacuucc gcaaccgggc gcucuaccug uacuacaccc agaugggccg cugcauguac     2520

acgggggaga ucaucgacau cgagcggcug uacgacaagg agcucuacga ccuggaccac     2580

aucauccccc gcaagaugaa gaaggacgac agcauccaca agaaccuggu gcucgucacc     2640

cggaacucca accaguacga gaagaaggac aacuaccccg ugcccgaggu gaucaagagc     2700

cgcgagaacg uccggcaccu gugguacgcc cugaagaaga agggccucau caccgaggag     2760

aaguacaacc gccugacccg gaucaacccg cuguccgaca aggagcucag cgaguucauc     2820

gagcgccagc ugguggagac gcgccagacc accaaggugg ucaaggaccu guucgagaac     2880

uacuacaccg acuccaagau cgugacggug aaggccgggc ucgucagcga gcugcggcgc     2940

gacuucgagg ugcugaagug ccgggagauc aacgaccuca accacgccca cgacgccuuc     3000

cugaacauca ucgugggcga cgucuggucc aagaaguuca ccagcaaccc cuacaacuuc     3060

gugaagaaga accgcgagaa guacacccug aacaacaucu ucaccaagaa ccagaaggac     3120

cuccggggga acgagaucug gaacugcgcg gagggcaaga agcgcaucau caagacgcug     3180

aacaagcccu ccgugcuguu cagcgucgag aacuucgagc agaccggcca gcuguucgac     3240

cagaacauca ucgggaagca ggacuacaag gagaagaccu ccuacgugcc caucaagggc     3300

gacgagcggc ugcaggacau cagcaaguac gggggcuacc gcggcaucuc cggggccuac     3360

uucuucaucg uggagcacac cgagaagaac aagcggguca agaccaucga gagccugccg     3420

aucuacauga aguccuacgu ggaggagaac ggccuccugg acuucugcac gaacgagaug     3480

aagcuggugg accccaagau caucgucaag aagcucaacu acaagagcga gaucaucgug     3540

gacggguuca aguaccugau cagcggcaag accgagaacc gccugucccu cgagaccaac     3600

gaguaccugu acuggaacca cacccagacg accgacauca agaagauccu gaacaucaac     3660

gagaagaucg agcucgagaa guacaacggg gacaucgaga gcgagcggga gaacaucauc     3720

aaguacaucc agaucauccu ggagaaguuc aagcugaagc ccuucaucaa gcgccggaac     3780

cuccccaaca ucgagaucaa cuucgccgac uacgagaccg agaagcugcu gcagcuccug     3840

gugaaccuga ucccgcucac cacgaaguac gucucccccg uggaccugac cgugaucagc     3900

ggcuccaagu acgccggcaa gguccugauc cccaagaaga ucagcaaccu caacgaggug     3960

aucuucaucu ccaagagcau caccgggauc uacgaguccc gccggcgcau caucuga        4017


<210>  1814
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1814
augaagcagg aguacuuccu gggccucgac auggggaccg gcagccuggg gugggccgug       60

accgacucca cguaccaggu caugcgcaag cacggcaagg cccugugggg gacccggcug      120

uucgagagcg cguccaccgc cgaggagcgc cggauguucc gcaccgcccg gcgccggcug      180

gaccgccgga acuggcgcau ccaggugcug caggagaucu ucagcgagga gaucuccaag      240

guggaccccg gcuucuuccu ccggaugaag gagagcaagu acuacccgga ggacaagcgc      300

gacgccgagg gcaacugccc cgagcugccc uacgcccugu ucgucgacga caacuacacg      360

gacaagaacu accacaagga cuaccccacc aucuaccacc uccggaagau gcugauggag      420

accaccgaga ucccggacau ccgccuggug uaccucgugc ugcaccacau gaugaagcac      480

cgggggcacu uccugcucuc cggcgacauc agccagauca aggaguucaa guccacguuc      540

gagcagcuga uccagaacau ccaggacgag gagcuggagu ggcacaucag ccucgacgac      600

gcggccaucc aguucgucga gcacgugcug aaggaccgca accugacccg guccaccaag      660

aagucccgcc ucaucaagca gcugaacgcc aagagcgccu gcgagaaggc gauccugaac      720

cuccugagcg ggggcaccgu gaagcugucc gacaucuuca acaacaagga gcucgacgag      780

agcgagcggc ccaaggucuc cuucgccgac agcggguacg acgacuacau cggcaucgug      840

gaggccgagc uggccgagca guacuacauc aucgccuccg cgaaggccgu guacgacugg      900

agcguccugg uggagauccu cggcaacagc guguccauca gcgaggccaa gaucaagguc      960

uaccagaagc accaggccga ccugaagacg cugaagaaga ucgugcgcca guacaugacc     1020

aaggaggacu acaagcgggu guucgucgac accgaggaga agcucaacaa cuacuccgcc     1080

uacaucggga ugaccaagaa gaacggcaag aagguggacc ucaagagcaa gcagugcacg     1140

caggcggacu ucuacgacuu ccugaagaag aacgugauca aggucaucga ccacaaggag     1200

aucacccagg agaucgaguc cgagaucgag aaggagaacu uccuccccaa gcaggucacc     1260

aaggacaacg gggugauccc cuaccaggug cacgacuacg agcugaagaa gauccuggac     1320

aaccucggca cccgcaugcc guucaucaag gagaacgccg agaagaucca gcagcuguuc     1380

gaguuccgca uccccuacua cgugggcccc cugaaccggg ucgacgacgg gaaggacggc     1440

aaguucacgu ggagcgugcg caaguccgac gcccggaucu accccuggaa cuucaccgag     1500

gugaucgacg ucgaggccag cgccgagaag uucauccgcc ggaugaccaa caagugcacc     1560

uaccucgugg gggaggacgu gcugccgaag gacucccugg ucuacagcaa guucauggug     1620

cucaacgagc ugaacaaccu gcgccucaac ggcgagaaga ucuccgugga gcugaagcag     1680

cggaucuacg aggagcuguu cugcaaguac cgcaagguca cgcggaagaa gcuggagcgc     1740

uaccucguga ucgaggggau cgcgaagaag ggcguggaga ucaccggcau cgacggggac     1800

uucaaggcca gccugaccgc cuaccacgac uucaaggagc ggcugaccga cguccagcuc     1860

ucccagcgcg ccaaggaggc caucgugcug aacguggucc uguucggcga cgacaagaag     1920

cuccugaagc agcggcugag caagauguac cccaaccuca cgaccgggca gcugaagggc     1980

aucugcuccc ugagcuacca gggguggggc cgccucucca agaccuuccu ggaggagauc     2040

accgugcccg cgcccggcac gggggaggug uggaacauca ugaccgcccu guggcagacc     2100

aacgacaacc ucaugcagcu gcugagccgg aacuacggcu ucaccaacga ggucgaggag     2160

uucaacacgc ucaagaagga gaccgaccug uccuacaaga ccguggacga gcuguacgug     2220

agcccggccg ucaagcgcca gaucuggcag acccucaagg uggugaagga gauccagaag     2280

gucaugggga acgcccccaa gcggguguuc guggagaugg cgcgcgagaa gcaggagggc     2340

aagcggagcg acucccgcaa gaagcagcug gucgagcugu accgcgccug caagaacgag     2400

gagcgggacu ggaucaccga gcucaacgcc cagagcgacc agcagcugcg cuccgacaag     2460

cuguuccucu acuacaucca gaaggggcgg ugcauguaca gcggcgagac gauccagcug     2520

gacgagcugu gggacaacac caaguacgac aucgaccaca ucuaccccca guccaagacc     2580

auggacgaca gccucaacaa ccgcgugcug gugaagaaga acuacaacgc caucaagucc     2640

gacaccuacc cccugagccu cgacauccag aagaagauga uguccuucug gaagaugcug     2700

cagcagcagg gcuucaucac gaaggagaag uacguccggc uggugcgcag cgacgagcuc     2760

uccgccgacg agcuggcggg guucaucgag cggcagaucg uggagacccg ccagagcacc     2820

aaggccgucg ccaccauccu gaaggaggcc cucccggaca cggagaucgu guacgugaag     2880

gccggcaacg ucuccaacuu ccggcagacc uacgagcugc ugaaggugcg cgagaugaac     2940

gaccuccacc acgcgaagga cgccuaccug aacaucgugg ucgggaacgc cuacuucgug     3000

aaguucacca agaacgccgc cugguucauc cggaacaacc ccggccgcag cuacaaccug     3060

aagcggaugu ucgaguucga caucgagcgc uccggcgaga ucgcguggaa ggccgggaac     3120

aagggcagca ucgugaccgu caagaaggug augcagaaga acaacauccu cgugacgcgg     3180

aaggccuacg aggucaaggg cgggcuguuc gaccagcaga ucaugaagaa ggggaagggc     3240

caggugccca ucaagggcaa cgacgagcgc cuggccgaca ucgagaagua cgggggcuac     3300

aacaaggcgg ccgggaccua cuucaugcuc gugaaguccc uggacaagaa gggcaaggag     3360

auccggacca ucgaguucgu cccccuguac cucaagaacc agaucgagau caaccacgag     3420

agcgccaucc aguaccuggc ccaggagcgc gggcugaaca gcccggagau ccuccugucc     3480

aagaucaaga ucgacacccu guucaaggug gacggcuuca agauguggcu cagcggccgg     3540

acggggaacc agcugaucuu caagggcgcc aaccagcuga uccucuccca ccaggaggcg     3600

gccauccuga aggggguggu caaguacgug aaccgcaaga acgagaacaa ggacgccaag     3660

cugagcgagc gggacggcau gaccgaggag aagcuccugc agcuguacga caccuuccuc     3720

gacaagcugu ccaacaccgu cuacagcauc cgccuguccg cccagaucaa gacgcucacc     3780

gagaagcgcg ccaaguucau cgggcugagc aacgaggacc agugcaucgu gcugaacgag     3840

auccuccaca uguuccagug ccaguccggc agcgcgaacc ugaagcugau cggcgggccc     3900

ggcuccgccg ggauccucgu gaugaacaac aacaucaccg ccugcaagca gaucagcguc     3960

aucaaccagu cccccaccgg caucuacgag aaggagaucg accugaucaa gcuguga        4017


<210>  1815
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1815
augaccaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

gugacggacg gguacaaggu gccggccaag aagaugaagg uccucggcaa caccgacaag      120

agccacauca agaagaaccu gcugggggcg cuccuguucg acuccggcaa caccgccaag      180

gaccgccggc ugaagcgcac cgcccggcgc cgguacacgc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucgc cgaggagaug gccaaggcgg acgagagcuu cuuccagcgc      300

cugaacgagu ccuuccucac caacgacgac aaggaguucg acagccaccc caucuucggc      360

aacaaggccg aggaggaggc ccaccaccac aaguucccca ccaucuucca ccuccggaag      420

caccuggccg acuccaccga gaaguccgac cugcgccuga ucuaccuggc gcucgcccac      480

augaucaagu uccgggggca cuuccugauc gagggccagc ugaaggccga gaacacgaac      540

gugcagaccc ucuucgacga cuucguggag gucuacgaca agaccgugga ggagagccac      600

cugagcgaga ucuccgugag cuccauccug accgagaaga ucagcaaguc ccgccggcuc      660

gagaaccuga ucaaguacua ccccacggag aagaagaaca cccuguucgg gaaccucauc      720

gcccugagcc ugggccucca gccgaacuuc aagaccaacu ucaagcuguc cgaggacgcc      780

aagcugcagu ucagcaagga caccuacgag gaggaccucg gggagcugcu gggcaagauc      840

ggcgacaacu acgcggaccu cuucaucucc gccaagaacc uguacgacgc cauccugcuc      900

agcgggaucc ugacgaucga cgacaacacc accaaggcgc cccugagcgc cuccaugauc      960

aagcgcuacg aggagcacca ggaggaccuc gcccagcuga agaaguucau ccggcagaac     1020

cugcccgacc aguacagcga ggucuucucc gacaagacca aggacggcua cgccggguac     1080

aucgacggca agacgaacca ggaggccuuc uacaaguaca ucaagaacau gcucagcaag     1140

accgagggcg ccgacuacuu ccuggacaag aucgaccgcg aggacuuccu gcggaagcag     1200

cgcaccuucg acaacggguc cgugccccac cagauccacc uccaggagau gcacgccauc     1260

cugcggcgcc agggcgagua cuacccguuc cugaaggaga accaggacaa gaucgagaag     1320

auccucaccu uccgcauccc cuacuacgug gggccccugg cgcggaaggg cagccgcuuc     1380

gccugggccg aguacaaggc cgacaagaag gucacgcccu ggaacuucga cgacauccug     1440

gacaaggaga aguccgccga ggaguucauc acccggauga cccugaacga ccucuaccug     1500

ccggaggaga aggugcugcc caagcacagc cucguguacg agaccuucaa cgucuacaac     1560

gagcugacga aggugaagua cgugaacgag caggggaagg cgaucuucuu cgacgccaac     1620

augaagcagg agaucuucga ccacgucuuc aaggagaacc gcaaggugac caaggacaag     1680

cugcucaacu accugaacaa ggaguucgag gaguuccgga ucgugaaccu gaccggccuc     1740

gacaaggaga acaaggccuu caacuccagc cugggcaccu accacgaccu gcgcaagauc     1800

cucgacaagu ccuuccugga cgacaagguc aacgagaaga ucaucgagga caucauccag     1860

acgcugaccc ucuucgagga ccgggagaug auccgccagc ggcugcagaa guacagcgac     1920

aucuucacca cccagcagcu gaagaagcuc gagcgccggc acuacacggg guggggccgc     1980

cuguccgcca agcugaucaa cgggauccgg gacaagcaga gcaacaagac cauccucggc     2040

uaccugaucg acgacgggua cuccaaccgc aacuucaugc agcugaucaa cgacgacagc     2100

cuccccuuca aggaggagau cgcgcgggcc caggugaucg gcgagaccga cgaccugaac     2160

cagcuggugu ccgacaucgc cggcagcccc gccaucaaga aggggauccu ccagucccug     2220

aagaucgucg acgagcuggu gaaggucaug ggccacaacc cggccaacau cgugaucgag     2280

auggcgcgcg agaaccagac caccgccaag gggcggcgca gcagccagca gcgguacaag     2340

cgccucgagg aggccaucaa gaaccuggac cacgaccuga accacaagau ccucaaggag     2400

caccccacgg acaaccaggc ccugcagaac gaccgccugu uccucuacua ccugcagaac     2460

ggccgggaca uguacaccga ggacccccug gacaucaacc gccucuccga cuacgacauc     2520

gaccacauca ucccccagag cuucaucaag gacgacucca ucgacaacaa ggugcugguc     2580

agcuccgcca agaaccgggg caagagcgac aacgugccgu ccgaggacgu ggucaaccgc     2640

augcggcccu ucuggaacaa gcugcucagc ugcgggcuga ucucccagcg caaguacagc     2700

aaccugacca agaaggagcu caagcccgac gacaaggcgg gcuucaucaa gcggcagcug     2760

guggagaccc gccagaucac gaagcacgug gcccagaucc uggacgcccg guucaacacc     2820

aagcgcgacg agaacaagaa ggucauccgg gacgugaaga ucaucacccu caaguccaac     2880

cuggugagcc aguuccgcaa ggacuucaag uucuacaagg uccgggagau caacgacuac     2940

caccacgccc acgacgccua ccugaacgcg gugaucggga aggcccuccu ggacguguac     3000

ccccagcugg agccggaguu cgucuacggc gaguaccccc acuuccacgg guacaaggag     3060

aacaaggcca ccgccaagaa guucuucuac uccaacauca ugaacuucuu caagaaggac     3120

gacauccgca cggacgagaa cggcgagauc guguggaaga aggacgagca caucagcaac     3180

aucaagcggg ugcucuccua cccccagguc aacaucguga agaaggugga gauccagacc     3240

gucggccaga acgggggccu guucgacgac aaccccaaga gcccgcugga ggugaccccc     3300

uccaagcucg ugccccugaa gaaggagcug aacccgaaga aguacggggg cuaccagaag     3360

cccaccacgg cguaccccgu ccuccugauc accgacacca agcagcugau ccccaucagc     3420

gugaugaaca agaagcaguu cgagcagaac ccggugaagu uccuccgcga ccggggguac     3480

cagcaggucg gcaagaacga cuucaucaag cugcccaagu acacccuggu ggacaucggc     3540

gacgggauca agcgccucug ggccagcucc aaggagaucc acaagggcaa ccagcuggug     3600

gucagcaaga agucccagau ccugcucuac cacgcccacc accuggacag cgaccugucc     3660

aacgacuacc uccagaacca caaccagcag uucgacgugc uguucaacga gaucaucagc     3720

uucuccaaga agugcaagcu ggggaaggag cacauccaga agaucgagaa cguguacagc     3780

aacaagaaga acuccgccag caucgaggag cucgccgagu ccuucaucaa gcugcugggc     3840

uucacgcagc ucggggcgac cagccccuuc aacuuccugg gcgucaagcu gaaccagaag     3900

caguacaagg gcaagaagga cuacauccuc cccugcaccg aggggacccu gauccggcag     3960

uccaucacgg gccuguacga gacccgcgug gaccucagca agaucgggga ggacuga        4017


<210>  1816
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1816
augcgcgacu acuacaucgg ccuggaccuc gggaccggca gccuggggug ggccgugacc       60

gaccgggagu acgagaucau gcgcgcccac ggcaaggcgc uguggggcgu ccggcuguuc      120

gacuccgcca acacggccga ggagcgccgg ggguuccgca ccgcccggcg ccggcuggac      180

cgccggaacu ggcgcaucga gcugcuccag gagcuguucg gcgaggacau cgggaaggug      240

gacagcggcu ucuuccugcg gaugaaggag uccaaguaca ugcccgagga caagcgcgac      300

gugaacggga acugcccgaa gcuccccuac gcccuguucg ucgaggacgg cuacaccgac      360

aaggacuacc accggcaguu ccccaccauc uaccaccucc gcaaguggcu cauggagacg      420

gaggagaccc ccgacauccg gcugguguac cuggcgcucc accacaugau gaagcaccgc      480

gggcacuucc uguucagcgg caacaucgag aagaucaagg aguuccagga gaccuuccgg      540

caguacaucg gcaagauccg cgaggaggag cuggacuucc accucugcau cgagggggag      600

gagcugcggg agaccgagaa cauccugaag gacaagaacc ucacgcgcuc cgccaagaag      660

acccgccuga ucaagcugcu cggcgcccac accgccugcg agaaggcggc ccugaaccug      720

guggccgggg gcaccgucaa gcucagcgac aucuucggca acuccgagcu ggacgccugc      780

gagaagccga agcugagcuu cgccgacgcg ggguacgacg acuacgccgg caugaucgag      840

gacgagcucg gggagcagca cgugaucauc gagacggcca aggccgugua cgacuggucc      900

guccuggccg acauccuggg cgacuaccgg ugcaucagcg aggcgaaggc cgccguguac      960

gagaagcacc agaaggaccu ccgccaccug aaggagcugg ugaaggagaa ccucgggcgg     1020

gacgucuaca aggagguguu cgugaagacc aacgagaagc ugcccaacua cuccgccuac     1080

aucggcauga ccaagaagaa cggcgucaag agcgagaugg aggggaagcg cugcgaccgg     1140

aaggccuucu acgacuaccu gaagaagacc guggugaacg cgauccccga cgaguccaag     1200

acggaguacc uccgcaagga gauggagacc gagaccuucc ugccccggca ggucaccaag     1260

gacaacggcg ugaucccgca ccaggugcac cuccaggagc uggacgccau ccuggagaac     1320

cugagcgggc gcauccccgc ccucaaggag aacggcucca agauccggga caucuucacg     1380

uuccgcaucc ccuacuacgu cgggccccug aacggcaucg ugaagggcgg ggagcggacc     1440

aacugggugc gccggaagaa ggccggccgc aucugcccgu ggaacuucga cgagaugguc     1500

gacaccgggg ccagcgcgga ggaguucauc cggcgcauga ccuccaagug cacguaccug     1560

auccacgagg acgugcuccc caagaacagc augcuguacu ccaaguucau gguccugaac     1620

gagcugaaca acgugcggcu caacggcgag cccaucagcg uggagcugaa gcagaagacc     1680

uacgaggacc uguuccagcg ccaccggaag gucacccgcc gccggcucac cgacuacauc     1740

cgccgggagg ggaucgccgg ccgcgacgcc gacaucacgg gcaucgacgg ggacuucaag     1800

ggcagccuga ccgccuacca cgacuucaag gagaagcuga ccgggugcga gcucucccag     1860

gcggacaagg agaacaucau ccugaacauc acccuguucg gcgaggacaa ggcccuccug     1920

aagaagcggc ugggcgcccu cuaccccgcc cugacggagc cgcagaagaa ggccaucugc     1980

gcgcugagcu acaaggggug gggccgccuc ucccagcggc ugcuggaggg gaucaccgcc     2040

cccgcccccg agaccggcga gaucuggacc gugauccgcg ccauguggga gacgaacgac     2100

aaccucaugc agguccugag cgagaaguac ugcuucgccg cggccaucga cgaggagaac     2160

gccggggagg agcugaagga gaucaccuac aagaccgugg agcagaugaa cguguccccc     2220

gccgugcggc gccagaucug gcagagccuc caggucauca aggagaucug caaggugaug     2280

ggcggcccgc ccaagcgggu guucgucgag auggcccgcg agaagaugga guccaagcgg     2340

accgagagcc gcaagaagcg gcugaucgac cuguacaaga agugccgcga ggaggagcgg     2400

gacuggaucg aggagcucgg gaacaccgag gagacgcgcc ugcgguccga caagcuguac     2460

cucuacuaca cccagaaggg ccgcugcaug uacagcgggg aggugaucga gcuggaggag     2520

cugugggaca accggaagua cgacaucgac cacaucuacc cccaguccaa ggugauggac     2580

gacagccucg acaaccgcgu ccuggugaag aaggaguaca acgcggacaa gaccgacgag     2640

uaccccaucc gcgccgacau ccggggcaag augcgcgccu ucuggcggau ccugcgcgag     2700

gagggguuca ucuccaagga gaaguacaac cggcucaccc gcggcacggg cuucgagccg     2760

agcgagcugg ccggguucau cgcgcggcag cugguggaga cccgccaggg caccaaggcc     2820

gucgccuccg ugcucaagca ggucuucccc gagaccgaca ucguguacgc caaggcccgg     2880

guggcgagcc aguuccgcca ggaguucgac cugaucaagg ugcgggagau gaacgaccug     2940

caccacgcca aggacgccua cgucaacauc guggugggga acgucuacua cacgaaguuc     3000

accuccaacg ccgccuggua cgugaaggag caccccggcc gcagcuacaa ccucaagaag     3060

auguucaccu ccgagcggga cguggcgcgc aacggggaga ccgccuggcg ggccggcaac     3120

agcggcacga ucgccaccgu caagcgcgug auggggaaga acaacauccu ggugacccgg     3180

cgcagcuacg aggucaaggg cgggcuguuc gaccagcagc ucaugaagaa gggcaagggc     3240

caggugccca ucaaggggcg ggacgagcgc cuggccgaca ucgacaagua cggcggguac     3300

aacaaggcgg ccggcaccua cuucaugcug gccgaguccg aggacaagaa gggggccaag     3360

auccgcagcg uggaguacgu cccgcucuac cugugcaacu gcaucgagaa ggacgaggag     3420

gcggccaaga aguaccugca gaaggagcgg ggccucaaga acccccgcgu gcugaucgcc     3480

aagaucaaga ucgacacgcu guucaaggug gacggcuucu acauguggcu cuccgggcgg     3540

accggcaacc agcugaucuu caagggggcc aaccagcuga uccucagcga gcccgacaug     3600

cgcauccuga agaagguccu gaaguacgug aaccggaaga aggagaacaa gaacgccgug     3660

cucggcgagc acgaccagcu gccggagacc gaccugaucc gccucuacga cgucuuccug     3720

gacaagaucg agaacaccgu guaccacgug cggcuguccg cgcagcaggg gacgcucacc     3780

aagaacaagg acaccuucug cgagcugagc aacgaggaca agugcaucgu ccuguccgag     3840

auccuccaca uguuccagug ccagagcggc uccgccaacc ugaagcugau caagggcccc     3900

gggagcgccg gcauccucgu gcugaacaac aucaucucca agugcaacca ggugagcauc     3960

auccaccagu cccccaccgg gaucuacgag caggagaucg accugaagaa gaucuga        4017


<210>  1817
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1817
augaacaagg ccuacacccu gggccucgac aucgggacca acagcguggg cugggccguc       60

gugacggacg acuaccgccu gauggcgaag aagaugcccg ugcacuccaa gauggagaag      120

aagaagauca agaagaacuu cuggggcgcc cggcuguucg acgaggggca gaccgccgag      180

gagcgccgga acaagcgcgc cacccggcgc cggcuccgcc ggcgcaagua ccggauccug      240

gagcugcaga agaucuucag cgaggagauc cucaagaagg acucccacuu cuucgcccgc      300

cuggacgaga gcuuccugau cccggaggac aagcaguacg cgcgguuccc caucuucccc      360

acccuccugg aggagaaggc cuacuaccag aacuacccca cgaucuacca ccuccgccag      420

aagcuggccg acuccaccga gaaggccgac auccggcugg ucuaccuggc gcucgcccac      480

augaucaagu accgcgggca cuuccuguuc gagggcgagc uggacaccga gaacaccagc      540

guggaggaga cguucaagga guucaucgac aucuacaacg agcaguucga ggagggcauc      600

aucuucuaca aggacauccc gcucauccug accgacaagc uguccaagag caagaaggug      660

gagaagaucc uccaguacua ccccaaggag aagaccaccg ggugccuggc ccaguuccug      720

aagcucaucg ucggcaacca ggggaacuuc aagcaggccu uccaccugga cgaggaggug      780

aagauccaga ucuccaagga gacguacgag gaggaccugg agaagcuccu gcgcaagagc      840

aacgaggaga ugaucgacgu guuccugcag gucaagaagg uguacgacgc cauccuccug      900

uccgacaucc ugagcaccaa gaugaaggac accaaggcga agcucuccgc cggcaugauc      960

gagcgguacc agaaccacaa gaaggaccug gaggagcuga agcaguucgu gcgggcccac     1020

cuccacgaga aggucaccgu guucuucaag gacagcucca aggacgggua cgccggcuac     1080

aucgacggca agacgaccca ggccgacuuc uacaaguucc ugaagaagga gcugaccggg     1140

gugcccggca gcgagcccau gcucgcgaag aucgaccagg agaacuuccu gcugaagcag     1200

cgcaccccga cgaacggggu caucccccac caggugcacc ucaccgaguu caaggccauc     1260

aucgaccagc agaagcagua cuaccccuuc cuggagaagu ccaaggagaa gaugauccag     1320

cugcucaccu uccggauccc cuacuacgug ggcccgcugg cccaggacaa ggagaccagc     1380

uccuucgccu ggcuggagcg caagacgacc gagaagauca agcccuggaa cgccaaggac     1440

gucaucgacu acggcgcgag cgccaccaag uucauccagc gcaugaucaa cuacgacacc     1500

uaccucccca cggagaaggu gcugcccaag uacagcaugc uguaccagaa guacaccauc     1560

uucaacgagc ugaccaaggu ggccuacaag gacgaccggg ggaucaagca ccaguucucc     1620

agcgaggaga agcuccgcau cuuccaggag cuguucaaga agcagcggcg cgucaccaag     1680

aagaagcugc agcacuuccu cuccgccaac uacaacaucg aggacgccga gauccugggc     1740

guggacaagg uguucaacag cuccuacgcg acguaccacg acuuccugga gcucgccaag     1800

ccguacaccg agaagaucau cgagcugcug gagcagcccg agauggagga gauguucgag     1860

gacaucguca agaucaucac caucuucgag gaccgggaga uggugcgcac ccagcucaag     1920

aaguaccagc ggauccuggg ggaggagauc uucaagaagc uggucaagaa gaaguacacg     1980

ggcugggggc gccucagcaa gcggcugauc aacggcaucc gcgaccagaa gaccaacaag     2040

accauccugg acuaccucau caacgacgac gacuuccccu acaaccggaa ccgcaacuuc     2100

augcagcuga ucaacgacga ccaccugucc uucaaggagg agaucgccaa ggagcucacc     2160

cugagcgaca agcagucccu gcucgaggug guggaggcca uccccggcag cccggcgauc     2220

aagaagggga ucuggcagac ccugaagauc gucgaggagc ugaucgccau caucggcuac     2280

aagcccaaga acaucgugau cgagauggcc cgggagaacc agacgaccac cgggggcaag     2340

aaccgcucca agccccggcu caagagccug gaggaggccc ugaagaacuu cgacucccag     2400

cuccugaagg agcgccccgu ggacaaccag agccugcaga aggaccggcu cuaccuguac     2460

uaccugcaga acgggaagga cauguacacc ggcgaguccc ucgacaucga ccgccugagc     2520

gaguacgaca ucgaccacau caucccgcgg uccuucaucg ucgaccacag ccuggacaac     2580

aaggugcucg uguccagcaa ggagaaccgc cugaagaagg acgacguccc cgacagcaag     2640

guggugaagc gcaugaaggc cuacugggag aagcugcucc gggcgaaccu gaucuccgag     2700

cgcaaguuca gcuaccugac gaagcucgag cugaccgacg acgacaaggc ccgguucauc     2760

cagcgccagc uggucgagac ccggcagauc accaagcacg uggccgccau ccuccaccag     2820

uacuucaacc agacgcagga gcuggagaag gagaaggaca uccgcaucau cacccugaag     2880

uccagccucg ugucccaguu ccggcagguc uucggcaucc acaaggugcg cgagaucaac     2940

caccaccacc acgcccacga cgcguaccug aacgccgugg ucgcccuggc ccuccugaag     3000

aaguaccccc ggcuggcccc cgaguucgug uacgggagcu ucgcgaaguu ccaccucgug     3060

aaggagaaca aggccaccgc caagaaggag uucuacucca acauccugaa guucuucgag     3120

aaggaggagc aguucugcga cgagaacggc gagaucuucu gggacaagcg caagcacauc     3180

cagcagauca agaaggucau cagcucccac caggugaaca ucgugaagaa ggucgaggug     3240

cagaccggga gcuucuacaa ggagacggug aacaccaagg agaagccgga caagcugauc     3300

aagcggaaga acaacuggga cgucaccaag uacggcgggu ucggcucccc cgugguggcc     3360

uacgcggucg uguucaccua cgagaagggc aagaaccaca agaaggccaa ggccaucgag     3420

gggaucacga ucauggagca ggcccucuuc gagaaggacc ccaucagcuu ccugaucgag     3480

aagggcuacu ccaacgugaa ccaguucauc aagcugccga aguacacccu cuucgagcug     3540

gccaacgggc agcgccggau gcuggcgagc caccaggagc uccagaaggc caacuccuuc     3600

auccugcccg agaagcuggu cacccuccug uaccacgcca accacuacga cgagaucgcc     3660

uacaaggaca gcuacgacua cgugaacgag cacuucucca acuuccagga cauccuggac     3720

aaggugauca ucuucgccga gaaguacacc agcgcgcccc agaagcucaa ccagaucauc     3780

gccacguacg agaagaacca ggaggccgac cgcaagauca uggcccacag cuucgucaac     3840

cugaugcagu ucaacgcccu gggcgcgccc gccgacuuca aguucuucga caccaccauc     3900

acccggaagc gcuacacguc ccucaccgag aucuggcaga gcaccaucau cuaccagucc     3960

gugaccggcc uguacgagac gcggcgccgg auggccgacc ugugggacgg ggugcaguga     4020


<210>  1818
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1818
auggagcgcu accacaucgg ccuggacauc gggaccagcu ccaucggcug ggccgugauc       60

ggggacgacu ucaagaucaa gaagaagaag ggcaagaacc ucaucgggac ccggcuguuc      120

aacgagggcg ccacggcggc cgagcgccgg ggcuuccgca cccagcggcg ccggcugaac      180

cgccggaagu ggcgccucaa gcugcuggag gagaucuucg accccuacau ggccgagguc      240

gacgaguacu ucuucgcccg gcucaagagc uccaaccuga gcccgaagga cuccaacaag      300

aaguaccugg ggagccuccu guuccccgac aaguccgaca gcaacuucua cgacaaguac      360

cccaccaucu accaccuccg ccgggaccug auggagaagg acgagaaguu cgaccugcgc      420

gagaucuacc uggccaucca ccacaucgug aaguaccggg gcaacuuccu cgagaaggug      480

cccgcgaaga acuacaagaa cuccggggcc agcaucggcu uccugcugga ggaggucaac      540

ucccucuaca aggacaucau cggggacgag agcaucgcca uccugaacuc cgagaaguuc      600

gaggacgugg agaagaucau ccuggacgag gagacccgca accucgacaa gcagaagucc      660

gugggcaagc ugcuggucga ggacaagaag aagaagaaca ucgugacggc cuucuccaag      720

gcgaucuucg gcuacaaguu caacaucgag gaccuccugc ugaucgagaa cgacgagaag      780

aacaagcuca ccuucaacga cgagaacauc gacgacaucu ucaacgagcu gagccacucc      840

cuggacgaca agcagaugga ccuccugacc aagacccggg agaucuacuu caaguucaag      900

cugaacauga ucgugccgac gggguacacc cucagcgagu ccaugaucga gaaguacgag      960

augcacaagg cccaccugaa gauguacaag gaguucauca acacccugaa caccaaggac     1020

cgcaagaucc ucaagaacgc cuacagcgac uacaucaaca cggagaaggc caaggccgcg     1080

aacgcccagg agaacuucua caagaccguc aagaagauca ucaaggacaa caacagcgac     1140

accgccaaga agaucaucgu gcugaucgac gagggcaacu ucaugcccaa gcagcggacc     1200

ggggagaacg gcgugauccc ccaccagcug caccagaucg agcucgaccg caucaucgag     1260

aaccaggcca aguacuaccc cuggcuggcc gaggcgaacc cggucgagaa gaaccggaag     1320

uucgccaagu acaagcugga cgagcucgug acguuccgcg ugcccuacua cgucggcccc     1380

cugaucgaca agaccgaguc caacaagaac gagaaggaga ccaaguucgc cuggauggug     1440

cgcaaggcca aggggaccau cacgcccugg aacuucgaga accuggugga ccggaccgag     1500

agcgccaacc gcuucaucaa gcggaugacc uccaaggaca ccuacaucau cggcgaggac     1560

guccucccgg cgagcucccu gcuguacgag aaguucaagg ugcucaacga gcugaacaac     1620

aucaagguga acgagaagaa gcuggacauc gagcagaagc aggacaucua ccugaaccuc     1680

uucacgaccg ccaagaacgu caccaagaag ucccuggcca ccuaccugaa cugcagcgcc     1740

gacagcaucu ccgggcucag cgacggcgag aaguucaacu ccagccuguc cagcuacauc     1800

gaccugaagg ccauccucgg gaacaucgug gacgacugca acaagaacga ggaccuggag     1860

aagaucaucg aguacuccac gguguucgag gacggcaaca ucuacaagga gaagcugagc     1920

gagaucuccu ggcucaccga cgagcagauc gagaagcuga gcaacaucca cuucaagggc     1980

ugggggcgcc uguccaagaa gcuccugacc cagaucacca acgagaacgg cgagcggauc     2040

aucgacgcgc uguggaacac gagcaacaac uucaugcaga ucaucaacga cgagagcauc     2100

caggccaagc ucgccgagau caacgggaag uacgccggca aguccgaccu ggaggacauc     2160

cugagcgagg cguacaccag cccccagaac aagaaggcca uccgccaggu caugaaggug     2220

gucgaggaca ucgagaaggc caugcagugc gagcccacca gcaucuccau cgaguucacc     2280

cggaagaagc agaagucccg ccucacgaac acccgguaca agaagaucag cgacguguac     2340

gagaagaucg ccgacgaggu gaucagcgag uacgagcugc gcaagcugca guccgagcuc     2400

gacagcaacg ccaacaacau gcgggaccgc uacuaccugu acuucaugca gcuggggcgg     2460

gacauguaca ccggcgagaa gaucaacauc gacgagcucc accagcgcua cgacaucgac     2520

cacauccugc cccaguccuu caucaaggac gacagcauca acaaccgggu ccugacccgc     2580

aaggacguga acaacaagga gaaguccgac aagaccgcgg ccgaccucua cgccguggag     2640

augagcgacu ucuggcggaa gcugcgcaag cagggccuga ucacggagca gaaguacaag     2700

aaccuccuga ccaaguccga cagcaucgac aaguacgcca agaagggguu caucaagcgg     2760

cagcuggucg agaccuccca gguggugaag cucgucgcca acauccugca ggacaaguac     2820

agcgacacca agaucaucga ggugcgcgcg aagcugaaca gcaaccuccg cgaggaguac     2880

aagcugauca agaaccgggc cgugaacgac uaccaccacg ccaucgacgg cuaccugacg     2940

accuucaucg ggcaguaccu cuacaagacc uacccgaagc ugcgcuccua cuucgucuac     3000

aacgacuuca agaagcugga cagcaacuac cucaagcaca ugaagaaguu caacuuccug     3060

uggcagcugc aggacgacaa gaccaucgac aucuacgaca acgugaacaa caaguucgug     3120

cucaacgucc ccgagaugaa gaaguacauc cagaagaucu acaacuacaa guacaugcug     3180

guguccaagg aggugacgac caagaacggc gccuucuacg accagaccaa guacaacgcc     3240

aagaccguca accugauccc caucaagaag aacaagccca cgaacaucua cgggggcuac     3300

aagggcaagg ugagcuccua caugaugcuc gugaagaucc ggaagaagaa ggaggucauc     3360

uacaaguucg ugggggugcc gcgccugugg accgacgagc uggaccggcu caaggacacc     3420

accgagcgca agauccugcu gaacgagauc gcgaagaaca gccucuccaa gacggagcag     3480

gacuucgagg ucauccugga caagguguac uacggccagc ugaucaucga cgggaaccag     3540

aaguacaccc ucggcagcuc cgaguacaag uacaacgcca ugcagcugca ccugagcaag     3600

cacgcccucg aggugcuggc caaggagaac gucaaggacg cggagaucac cgacaaggac     3660

cugguguccg uguacgagga gauccucagc gucgugaaca aguacuucga gcuguacgac     3720

aucaacaagu uccggcagaa gcugaacgag ggccucgaga ucuuccgcaa gcugcccgug     3780

uacaacgucu acgaguccaa caagaucaag caggucggga aguucgaggu gcugaaccgg     3840

auccucaugg gccugcacgc caacgccaug aucaccgacc ugaaggugcu cgggaucaag     3900

acgaagcugg gccagaugca ggucaacggg ggcaucaagc ugagccccga cgccaagcuc     3960

aucuaccagu cccccaccgg caucuucagc cgcgccgugc gggugaagga ccugggguga     4020


<210>  1819
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1819
augagccuga acaccaacaa gaaguacaac aucggccucg acaucgggac cacguccgug       60

ggcugggccg ucaccgacgc cgacgggcag aagcugcuga aggcgaagaa caagaaccuc      120

uggggcguga gccuguucga ggccgggaag accgccgccg agcgccgggg cuaccgcucc      180

acccggcgcc ggcugaacca ccgcaaguuc cggcuccgcc ugcuggagga cauguucgag      240

aaggagaucc ucagcaagga ccccuccuuc uucauccggc ugaaggaggc cuuccugagc      300

ccgaaggacg agcagaagca guucaagggc aacauccucu ucaacgacaa ggacuacacg      360

gacgcggacu acuacgagca guacaagacc aucuaccacc uccgcuacga ccugaucucc      420

cagcaccggc aguucgacau ccgcgaggug uaccucgcca uccaccaccu gaucaaguac      480

cgggggcacu ucaucuacga ggaccagacc uucaccacgg acggcaacca gcugcagcac      540

cacaucaagg ccaucaucac caugaucaac agcauccuca accgcaucau cauccccgag      600

accaucgaca ucaacgucuu cgagaagacc cugcuggacc ggacgaugaa ccgcuccagc      660

aaggugaagu uccucaucga gcugaccggg gaggacaagc aggacaagcc ccugcucaag      720

gagcuguuca accugaucgu gggccucaag gccaagcccg cguccaucuu cgagcaggag      780

aaccuggcca ccaucgucga gaccaugaac augagcacgg agcaggucca gcuggaccuc      840

cugacccugg ccgacgugcu cgccgacgag gaguacgacc ugcugcucac cgcccagaag      900

aucuacuccg cgaucauccu ggacgagagc auggacgggu acgaguacuu cgccgaggcc      960

aagaaggagu ccuaccggaa gcaccaggag gagcuggugc ucgugaagaa gaugcucaag     1020

agcaacgcca ucaccaacga cgagcgcgcc aaguucgagu acuucuacac ggacuacauc     1080

ggcgcgaagu ccagcaacua cgaggagucc aagaucauca agaagggccu gagcgccgcc     1140

uacgggaagu acagcaagga ggagcggcug uucaagcaca ucgagcugcu gcucgccaag     1200

gagaacgugc ugaccaccgu cgagcacgac cugcucgaga agaacaucac cuucgccucc     1260

cugcugccgc ugcagcgcag cucccggaac gcggugaucc ccuaccaggu gcacgagaag     1320

gagcucgucg ccauccugga gaaccaggcc acguacuacc ccuuccugcu cgagcagaag     1380

gacaacaucc acaagcugcu gaccuuccgc auccccuacu acgugggccc gcucgccgac     1440

cagaaggaca gcgaguucgc cuggauggug cgcaagcagg cggggaagau cacccccuuc     1500

aacuucgagg agauggucga caucgacgcc uccagcgagg ccuucaucaa gcggaugacc     1560

aacaagugca cguaccugau ccacgaggac gugaucccca agcacuccuu cagcuacgcc     1620

aaguucgagg ugcugaacga gcucaacaag auccgccugg acggcaagcc caucgacauc     1680

ccgcugaaga agcggaucuu cgagggccuc uuccuggaga agaccaaggu cacccacacc     1740

ucccugaaga aguggcucgc ggaguacgag cacaugacgg ugagcguggu ccaggggacc     1800

cagaaggaga ccgaguucgc caccucccug caggccuucc accgcuucgu gaagaucuuc     1860

gaccgggaga cggucagcaa ccccgccaac gaggagaugu ucgagaagau caucuacugg     1920

uccaccgugu ucgaggacaa gaagaucaug cgccggaagc ugagcgagua cccccagcuc     1980

accgagcagc agcaggucca gcuggcccag gugcgcuucc ggggcugggg gcgccugucc     2040

cagcggcuca ucaaccgcau caagaccccc gugagcggcg acgaggacca caagcugucc     2100

aucaacgaga uccuguggca gaccaacgag aacuucaugc agaucauccg gaacaaggac     2160

uaccucuuca agaagaucau cgaggagcag uucgagaacg agacggcgcu gcugaacaag     2220

cagcgcaucg acgagcucgc caccagcccg gccaacaaga aggggaucug gcaggccauc     2280

aagaucguga aggagcugga gaagguccug cagcagcccg ccgagaacau cuucaucgag     2340

uucgcgcggu ccgacgagga gagcaagcgc agcacgcccc gggacaaguu caucgagaag     2400

gccuacgccc agcucaagaa ggagaccgac accuucaacc uggagcaccu gaaggagcuc     2460

aagcagcgcu ccaagcagcu gagcucccag cggcuguucc ucuacuucau ccagaacggc     2520

aagugcaugu acagcggcga gcaccuggac aucgagcgcc uggacuccua cgagguggac     2580

cacauccucc cccagagcua caucaaggac aacuccaucg agaaccuggc ccuggugaag     2640

aaggucgaga accagcgcaa gaaggacagc cuccugcuga acuccagcau caucaaccag     2700

aacuacuccc ggugggagca gcucaagaac gccgggcuga ucggcgagaa gaaguuccgc     2760

aaccugaccc ggaccaagau caccgaccgc gacaaggagg gguucaucgc gcggcagcuc     2820

guggagacgc gccagaucac caagcacgug acccagcugc ugcagcagga guacaaggac     2880

accacgaagg ucuucgccau caaggccacc cucgugagcg accugcggcg caaguucgag     2940

uucaucaaga accggaacgu gaacgacuac caccacgccc aggacgcguu ccuggucgcc     3000

uuccucggca ccaacaucac cuccaacuac ccgaagaucg agauggagua ccuguucaag     3060

ggguaccagc acuaccugaa cgaggugggc aagagcgccg ccaagcccaa guucacguuc     3120

aucguggaga accucuccaa gcagcaguac aacagcacca ccggcgaggu caaguggaac     3180

cccgaggugg acaucgccaa gcugaagcgc auccugaacu ucaagcagug caacaucgug     3240

cggaaggucg aggagcaguc cggggcgcug uucaaggaga ccaucuaccc cguggaggag     3300

agcagcucca agacgauccc gcugaagaag caccuggaca ccgccaucua cggcggguac     3360

accgccguga acuacgccag cuacgcccuc auccaguuca agaagggccg caagcugaag     3420

cgggagauca ucgggauccc ccuggcgguc cagacccgca ucgacaacuc cgagacgagc     3480

cuccagaccu acaucgccga gcagaucaag uccgaggugg agauccugaa cggccggauc     3540

cugaaguacc agcucaucag caacaacggc aaccgccugu acaucgccgg gccccgggag     3600

cgccacaacg cccggcagcu gaucgugucc gacgaggccg cgaaggucau cuggcucauc     3660

agcaccaagc aggccgugga ggccaccuuc cugaagcacu acacgcugga gcaccucgag     3720

gccguguucg aggagcugau ccgcaagcag gcggccgacu accagaucuu cgagaaccug     3780

aucaagaaga ucgaggucaa caaggccuac uucuacuccu gcaccuacaa cgagaaggug     3840

aaggugaucg aggagcuccu gaagaucacc caggccaacg ccaccaacgg cgaccugaag     3900

cuccugaaga ugacgaaccg ggaggggcgc cugggcagcg ucuccguggc gcucgacuuc     3960

aagaucauca accagagcgu gaccggccug uaccagucca ucgaggacua caacaacuga     4020


<210>  1820
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1820
augagccuga acaccaacaa gaaguacaac aucggccucg acaucgggac cacguccgug       60

ggcugggccg ucaccgacgc cgacgggcag aagcugcuga aggcgaagaa caagaaccuc      120

uggggcguga gccuguucga ggccgggaag accgccgccg agcgccgggg cuaccgcucc      180

acccggcgcc ggcugaacca ccgcaaguuc cggcuccgcc ugcuggagga cauguucgag      240

aaggagaucc ucagcaagga ccccuccuuc uucauccggc ugaaggaggc cuuccugagc      300

ccgaaggacg agcagaagca guucaagggc aacauccucu ucaacgacaa ggacuacacg      360

gacgcggacu acuacgagca guacaagacc aucuaccacc uccgcuacga ccugaucucc      420

cagcaccggc aguucgacau ccgcgaggug uaccucgcca uccaccaccu gaucaaguac      480

cgggggcacu ucaucuacga ggaccagacc uucaccacgg acggcaacca gcugcagcac      540

cacaucaagg ccaucaucac caugaucaac agcauccuca accgcaucau cauccccgag      600

accaucgaca ucaacgucuu cgagaagacc cugcuggacc ggacgaugaa ccgcuccagc      660

aaggugaagu uccucaucga gcugaccggg gaggacaagc aggacaagcc ccugcucaag      720

gagcuguuca accugaucgu gggccucaag gccaagcccg cguccaucuu cgagcaggag      780

aaccuggcca ccaucgucga gaccaugaac augagcacgg agcaggucca gcuggaccuc      840

cugacccugg ccgacgugcu cgccgacgag gaguacgacc ugcugcucac cgcccagaag      900

aucuacuccg cgaucauccu ggacgagagc auggacgggu acgaguacuu cgccgaggcc      960

aagaaggagu ccuaccggaa gcaccaggag gagcuggugc ucgugaagaa gaugcucaag     1020

agcaacgcca ucaccaacga cgagcgcgcc aaguucgagu acuucuacac ggacuacauc     1080

ggcgcgaagu ccagcaacua cgaggagucc aagaucauca agaagggccu gagcgccgcc     1140

uacgggaagu acagcaagga ggagcggcug uucaagcaca ucgagcugcu gcucgccaag     1200

gagaacgugc ugaccaccgu cgagcacgac cugcucgaga agaacaucac cuucgccucc     1260

cugcugccgc ugcagcgcag cucccggaac gcggugaucc ccuaccaggu gcacgagaag     1320

gagcucgucg ccauccugga gaaccaggcc acguacuacc ccuuccugcu cgagcagaag     1380

gacaacaucc acaagcugcu gaccuuccgc auccccuacu acgugggccc gcucgccgac     1440

cagaaggaca gcgaguucgc cuggauggug cgcaagcagg cggggaagau cacccccuuc     1500

aacuucgagg agauggucga caucgacgcc uccagcgagg ccuucaucaa gcggaugacc     1560

aacaagugca cguaccugau ccacgaggac gugaucccca agcacuccuu cagcuacgcc     1620

aaguucgagg ugcugaacga gcucaacaag auccgccugg acggcaagcc caucgacauc     1680

ccgcugaaga agcggaucuu cgagggccuc uuccuggaga agaccaaggu cacccacacc     1740

ucccugaaga aguggcucgc ggagcacgag cacaugacgg ugagcguggu ccaggggacc     1800

cagaaggaga ccgaguucgc caccucccug caggccuucc accgcuucgu gaagaucuuc     1860

gaccgggaga cggucagcaa ccccgccaac gaggagaugu ucgagaagau caucuacugg     1920

uccaccgugu ucgaggacaa gaagaucaug cgccggaagc ugagcgagua cccccagcuc     1980

accgagcagc agcaggucca gcuggcccag gugcgcuucc ggggcugggg gcgccugucc     2040

cagcggcuca ucaaccgcau caagaccccc gugagcggcg acgaggacca caagcugucc     2100

aucaacgaga uccuguggca gaccaacgag aacuucaugc agaucauccg gaacaaggac     2160

uaccucuuca agaagaucau cgaggagcag uucgagaacg agacggcgcu gcugaacaag     2220

cagcgcaucg acgagcucgc caccagcccg gccaacaaga aggggaucug gcaggccauc     2280

aagaucguga aggagcugga gaagguccug cagcagcccg ccgagaacau cuucaucgag     2340

uucgcgcggu ccgacgagga gagcaagcgc agcacgcccc gggacaaguu caucgagaag     2400

gccuacgccc agcucaagaa ggagaccgac accuucaacc uggagcaccu gaaggagcuc     2460

aagcagcgcu ccaagcagcu gagcucccag cggcuguucc ucuacuucau ccagaacggc     2520

aagugcaugu acagcggcga gcaccuggac aucgagcgcc uggacuccua cgagguggac     2580

cacauccucc cccagagcua caucaaggac aacuccaucg agaaccuggc ccuggugaag     2640

aaggucgaga accagcgcaa gaaggacagc cuccugcuga acuccagcau caucaaccag     2700

aacuacuccc ggugggagca gcucaagaac gccgggcuga ucggcgagaa gaaguuccgc     2760

aaccugaccc ggaccaagau caccgaccgc gacaaggagg gguucaucgc gcggcagcuc     2820

guggagacgc gccagaucac caagcacgug acccagcugc ugcagcagga guacaaggac     2880

accacgaagg ucuucgccau caaggccacc cucgugagcg accugcggcg caaguucgag     2940

uucaucaaga accggaacgu gaacgacuac caccacgccc aggacgcguu ccuggucgcc     3000

uuccucggca ccaacaucac cuccaacuac ccgaagaucg agauggagua ccuguucaag     3060

ggguaccagc acuaccugaa cgaggugggc aagagcgccg ccaagcccaa guucacguuc     3120

aucguggaga accucuccaa gcagcaguac aacagcacca ccggcgaggu caaguggaac     3180

cccgaggugg acaucgccaa gcugaagcgc auccugaacu ucaagcagug caacaucgug     3240

cggaaggucg aggagcaguc cggggcgcug uucaaggaga ccaucuacag cguggaggag     3300

agcuccagca agacgauccc ccugaagaag caccuggaca ccgccaucua cggcggguac     3360

accgccguga acuacgccuc cuacgcccuc auccaguuca agaagggccg caagcugaag     3420

cgggagauca ucgggauccc gcuggcgguc cagacccgca ucgacaacag cgagacgucc     3480

cuccagaccu acaucgccga gcagaucaag agcgaggugg agauccugaa cggccggauc     3540

cugaaguacc agcucaucuc caacaacggc aaccgccugu acaucgccgg gccccgggag     3600

cgccacaacg cccggcagcu gaucgugagc gacgaggccg cgaaggucau cuggcucauc     3660

uccaccaagc aggccgugga ggccaccuuc cugaagcacu acacgcugga gcaccucgag     3720

gccguguucg aggagcugau ccgcaagcag gcggccgacu accagaucuu cgagaaccug     3780

aucaagaaga ucgaggucaa caaggccuac uucuacagcu gcaccuacaa cgagaaggug     3840

aaggugaucg aggagcuccu gaagaucacc caggccaacg ccaccaacgg cgaccugaag     3900

cuccugaaga ugacgaaccg ggaggggcgc cugggcuccg ucagcguggc gcucgacuuc     3960

aagaucauca accaguccgu gaccggccug uaccagagca ucgaggacua caacaacuga     4020


<210>  1821
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1821
augaagaagg aguacaccau cggccuggac aucgggacca acagcguggg cugguccguc       60

cucacggacg acuaccgccu ggugagcaag aagaugaagg uggccgggaa caccgagaag      120

uccagcacca agaagaacuu cuggggcguc cggcuguucg acgaggggca gaccgccgag      180

gcgcgccggu ccaagcgcac ggcccggcgc cggcucgccc gccggcgcca gcggauccug      240

gagcugcaga agaucuucgc ccccgagauc cucaagaucg acgagcacuu cuucgcccgc      300

cugaacgaga gcuuccuggu gcucgacgag aagaagcagu cccggcaccc gguguucgcg      360

accaucaagc aggagaaguc cuaccaccag accuacccca ccaucuacca ccuccgccag      420

gcccuggccg acagcagcga gaaggccgac auccggcucg ucuaccuggc gauggcccac      480

cuccugaagu accgcggcca cuuccugauc gagggcgagc ugaacacgga gaacuccagc      540

gugaccgaga ccuuccggca guuccucucc accuacaacc agcaguucag cgaggccgac      600

gacaagcaga cggagaagcu ggacgaggcc guggacugcu ccuucgucuu caccgagaag      660

augagcaaga ccaagaaggc cgagacccug cucaaguacu ucccccacga gaaguccaac      720

ggguaccuga gccaguucau caagcugaug gugggcaacc aggggaacuu caagaacgug      780

uucggccucg aggaggcgaa gcugcaguuc uccaaggaga cguacgagga ggaccuggag      840

gagcuccugg agaagaucgg ggacgacuac aucgaccugu ucguccaggc caagaacgug      900

uacgacgccg ugcuccugag cgagauccug agcgacucca ccaagaacac ccgcgccaag      960

cucagcgccg gcaugauccg gcgcuacgac gcgcacaagg aggaccuggu ccugcucaag     1020

cgguucguga aggagaaccu gcccaagaag uaccgcgccu ucuucggcga caacuccgug     1080

aacggguacg ccggcuacau cgaggggcac gccacccagg aggacuucua caaguucguc     1140

aagaaggagc ugacgggcau ccgcggcagc gagguguucc ucaccaagau cgagcaggag     1200

aacuuccugc ggaagcagcg caccuucgac aacgggguga ucccgcacca gauccaccug     1260

accgagcucc gggccaucau cgcgaaccag aagaagcacu accccuuccu gaaggaggag     1320

caggagaagc uggagucccu ccugacguuc aagauccccu acuacgucgg cccccuggcc     1380

aagaagcagg agaacagccc guucgccugg cucauccgca aguccgagga gaagaucaag     1440

cccuggaacc ugcccgagau cguggacaug gaggggagcg ccgugcgguu caucgagcgc     1500

augaucaaca ccgacaugua caugccccac aacaaggucc ugccgaagaa cucccugcuc     1560

uaccagaagu ucagcaucua caacgagcug accaaggugc gguaccagga cgagcgcggc     1620

cagaugaacu acuucuccag caucgagaag aaggagaucu uccacgagcu guucgagaag     1680

aaccggaagg ucaccaagaa ggaccuccag gaguuccugu accugaagua cgacaucaag     1740

cacgccgagc ucuccgggau cgagaaggcg uucaacgcca gcuacacgac cuaccacgac     1800

uuccugacca uguccgagaa caagcgcgag augaagcagu ggcuggagga ccccgagcuc     1860

gccagcaugu ucgaggagau caucaagacc cugacggugu ucgaggaccg ggagaugauc     1920

aagacccgcc ugucccacca cgaggccacc cucggcaagc acaucaucaa gaagcugacc     1980

aagaagcacu acacgggcug ggggcggcug agcaaggagc ucauccaggg cauccgcgac     2040

aagcagucca acaagaccau ccuggacuac cugaucaacg acgacgacuu cccccaccac     2100

cggaaccgca acuucaugca gcucaucaac gacgacagcc ugagcuucaa gaaggagauc     2160

aagaaggcgc agaugaucac cgacaccgag aaccuggagg agaucgugaa ggagcucacc     2220

gggucccccg ccaucaagaa gggcauccug cagagccuga agaucgucga cgagaucgug     2280

gggaucaugg gcuacgagcc ggccaacauc guggucgaga uggcccggga gaaccagacg     2340

accggccgcg ggcucaaguc cagccggccc cgccugaagg cccuggagga gucccucaag     2400

gacuucggca gccagcugcu gaaggaguac cccaccgaca acuccagccu ccagaaggac     2460

cgccuguacc uguacuaccu ccagaacggg cgggacaugu acaccggcgc gccccuggac     2520

auccaccgcc uguccgacua cgacaucgac cacaucaucc cgcggagcuu cacgaccgac     2580

aacuccaucg acaacaaggu gcucgugagc uccaaggaga accgccugaa gaaggacgac     2640

guccccagcg agaagguggu gaagaagaug cgguccuucu gguacgaccu guacagcucc     2700

aagcucauca gcaagcgcaa gcuggacaac cugaccaaga ucaagcucac cgaggaggac     2760

aaggccgggu ucaucaagcg gcagcugguc gagacgcgcc agaucaccaa gcacguggcc     2820

ggcauccugc accaccgguu caacaaggcc gaggacacca acgagcccau ccgcaaggug     2880

cggaucauca cccucaagag cgcccugguc ucccaguucc gcaaccgguu cggcaucuac     2940

aaggugcgcg agaucaacga guaccaccac gcgcacgacg ccuaccugaa cggggugguc     3000

gcccucgccc ugcugaagaa guacccccag cucgccccgg aguucgugua cggcgaguac     3060

cugaaguuca acgcgcacaa ggccaacaag gccacgguga agaaggaguu cuacagcaac     3120

aucaugaagu ucuucgaguc cgacaccccc gucugcgacg agaacgggga gaucuucugg     3180

gacaagagca aguccaucgc ccaggugaag aaggugauca accaccacca caugaacauc     3240

gucaagaaga ccgagaucca gaagggcggc uucagcaagg agaccgugga gcccaagaag     3300

gacuccagca agcugcuccc ccggaagaac aacugggacc cggcgaagua cgggggccug     3360

ggguccccca acguggccua cacggucgcc uucaccuacg agaagggcaa ggcccgcaag     3420

cggaccaacg cccuggaggg gaucaccauc auggagcgcg aggcguucga gcagagcccc     3480

gugcuguucc ugaagaacaa gggcuacgag caggccgaga ucgagaugaa gcugccgaag     3540

uacgcccucu ucgagcugga gaacggccgg aagcgcaugg uggccuccaa caaggaggcc     3600

cagaaggcga acagcuuccu gcuccccgag caccugguca cgcugcucua ccacgccaag     3660

caguacgacg agaucuccca caaggagagc uucgacuacg ugaacgagca ccacaaggag     3720

uucuccgagg uguucgcccg gguccuggag uucgccggga aguacacccu ggccgagaag     3780

aacaucgaga agcucgagaa gaucuacaag gagaaccaga ccgacgaccu ggcgaagcug     3840

gccagcuccu ucgugaaccu caugcaguuc aacgccaugg gcgcccccgc ggacuucaag     3900

uucuucgacg ugaccauccc ccgcaagcgc uacacgagcc ugaccgagau cuggcagucc     3960

accaucaucc accagagcau caccgggcug uacgagacgc ggauccgcau gggcaaguga     4020


<210>  1822
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1822
augaagaagg aguacaccau cggccuggac aucgggacca acagcguggg cugguccguc       60

cucacggacg acuaccgccu ggugagcaag aagaugaagg uggccgggaa caccgagaag      120

uccagcacca agaagaacuu cuggggcguc cggcuguucg acgaggggca gaccgccgag      180

gcgcgccggu ccaagcgcac ggcccggcgc cggcucgccc gccggcgcca gcggauccug      240

gagcugcaga agaucuucgc ccccgagauc cucaagaucg acgagcacuu cuucgcccgc      300

cugaacgaga gcuuccuggu gcucgacgag aagaagcagu cccggcaccc gguguucgcg      360

accaucaagc aggagaaguc cuaccaccag accuacccca ccaucuacca ccuccgccag      420

gcccuggccg acagcagcga gaaggccgac auccggcucg ucuaccuggc gauggcccac      480

cuccugaagu accgcggcca cuuccugauc gagggcgagc ugaacacgga gaacuccagc      540

gugaccgaga ccuuccggca guuccucucc accuacaacc agcaguucag cgaggccgac      600

gacaagcaga cggagaagcu ggacgaggcc guggacugcu ccuucgucuu caccgagaag      660

augagcaaga ccaagaaggc cgagacccug cucaaguacu ucccccacga gaaguccaac      720

ggguaccuga gccaguucau caagcugaug gugggcaacc aggggaacuu caagaacgug      780

uucggccucg aggaggaggc gaagcugcag uucuccaagg agacguacga ggaggaccug      840

gaggagcucc uggagaagau cggggacgac uacaucgacc uguucgucca ggccaagaac      900

guguacgacg ccgugcuccu gagcgagauc cugagcgacu ccaccaagaa cacccgcgcc      960

aagcucagcg ccggcaugau ccggcgcuac gacgcgcaca aggaggaccu gguccugcuc     1020

aagcgguucg ugaaggagaa ccugcccaag aaguaccgcg ccuucuucgg cgacaacucc     1080

gugaacgggu acgccggcua caucgagggg cacgccaccc aggaggacuu cuacaaguuc     1140

gucaagaagg agcugacggg cauccgcggc agcgaggugu uccucaccaa gaucgagcag     1200

gagaacuucc ugcggaagca gcgcaccuuc gacaacgggg ugaucccgca ccagauccac     1260

cugaccgagc uccgggccau caucgcgaac cagaagaagc acuaccccuu ccugaaggag     1320

gagcaggaga agcuggaguc ccuccugacg uucaagaucc ccuacuacgu cggcccccug     1380

gccaagaagc aggagaacag cccguucgcc uggcucaucc gcaaguccga ggagaagauc     1440

aagcccugga accugcccga gaucguggac auggagggga gcgccgugcg guucaucgag     1500

cgcaugauca acaccgacau guacaugccc cacaacaagg uccugccgaa gaacucccug     1560

cucuaccaga aguucagcau cuacaacgag cugaccaagg ugcgguacca ggacgagcgc     1620

ggccagauga acuacuucuc cagcaucgag aagaaggaga ucuuccacga gcuguucgag     1680

aagaaccgga aggucaccaa gaaggaccuc caggaguucc uguaccugaa guacgacauc     1740

aagcacgccg agcucuccgg gaucgagaag gcguucaacg ccagcuacac gaccuaccac     1800

gacuuccuga ccauguccga gaacaagcgc gagaugaagc aguggcugga ggaccccgag     1860

cucgccagca uguucgagga gaucaucaag acccugacgg uguucgagga ccgggagaug     1920

aucaagaccc gccuguccca ccacgaggcc acccucggca agcacaucau caagaagcug     1980

accaagaagc acuacacggg cugggggcgg cugagcaagg agcucaucca gggcauccgc     2040

gacaagcagu ccaacaagac cauccuggac uaccugauca acgacgacga cuucccccac     2100

caccggaacc gcaacuucau gcagcucauc aacgacgaca gccugagcuu caagaaggag     2160

aucaagaagg cgcagaugau caccgacacc gagaaccugg aggagaucgu gaaggagcuc     2220

accggguccc ccgccaucaa gaagggcauc cugcagagcc ugaagaucgu cgacgagauc     2280

guggggauca ugggcuacga gccggccaac aucguggucg agauggcccg ggagaaccag     2340

acgaccggcc gcgggcucaa guccagccgg ccccgccuga aggcccugga ggagucccuc     2400

aaggacuucg gcagccagcu gcugaaggag uaccccaccg acaacuccag ccuccagaag     2460

gaccgccugu accuguacua ccuccagaac gggcgggaca uguacaccgg cgcgccccug     2520

gacauccacc gccuguccga cuacgacauc gaccacauca ucccgcggag cuucacgacc     2580

gacaacucca ucgacaacaa ggugcucgug agcuccaagg agaaccgccu gaagaaggac     2640

gacgucccca gcgagaaggu ggugaagaag augcgguccu ucugguacga ccuguacagc     2700

uccaagcuca ucagcaagcg caagcuggac aaccugacca agaucaagcu caccgaggag     2760

gacaaggccg gguucaucaa gcggcagcug gucgagacgc gccagaucac caagcacgug     2820

gccggcaucc ugcaccaccg guucaacaag gccgaggaca ccaacgagcc cauccgcaag     2880

gugcggauca ucacccucaa gagcgcccug gucucccagu uccgcaaccg guucggcauc     2940

uacaaggugc gcgagaucaa cgaguaccac cacgcgcacg acgccuaccu gaacggggug     3000

gucgcccucg cccugcugaa gaaguacccc cagcucgccc cggaguucgu guacggcgag     3060

uaccugaagu ucaacgcgca caaggccaac aaggccacgg ugaagaagga guucuacagc     3120

aacaucauga aguucuucga guccgacacc cccgucugcg acgagaacgg ggagaucuuc     3180

ugggacaaga gcaaguccau cgcccaggug aagaagguga ucaaccacca ccacaugaac     3240

aucgucaaga agaccgagau ccagaagggc ggcuucagca aggagaccgu ggagcccaag     3300

aaggacucca gcaagcugcu cccccggaag aacaacuggg acccggcgaa guacgggggc     3360

cugggguccc ccaacguggc cuacacgguc gccuucaccu acgagaaggg caaggcccgc     3420

aagcggacca acgcccugga ggggaucacc aucauggagc gcgaggcguu cgagcagagc     3480

cccgugcugu uccugaagaa caagggcuac gagcaggccg agaucgagau gaagcugccg     3540

aaguacgccc ucuucgagcu ggagaacggc cggaagcgca ugguggccuc caacaaggag     3600

gcccagaagg cgaacagcuu ccugcucccc gagcaccugg ucacgcugcu cuaccacgcc     3660

aagcaguacg acgagaucuc ccacaaggag agcuucgacu acgugaacga gcaccacaag     3720

gaguucuccg agguguucgc ccggguccug gaguucgccg ggaaguacac ccuggccgag     3780

aagaacaucg agaagcucga gaagaucuac aaggagaacc agaccgacga ccuggcgaag     3840

cuggccagcu ccuucgugaa ccucaugcag uucaacgcca ugggcgcccc cgcggacuuc     3900

aaguucuucg acgugaccau cccccgcaag cgcuacacga gccugaccga gaucuggcag     3960

uccaccauca uccaccagag caucaccggg cuguacgaga cgcggauccg caugggcaag     4020

uga                                                                   4023


<210>  1823
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1823
augaagaccg acuacuucau gggccuggac auggggaccg gcagccucgg gugggccgug       60

acggacucca gcuaccaccu gugccgcgcc cacggcaagg accugugggg cguccggcug      120

uucgagaccg cgcagaccgc cgaggagcgc cggguguucc gcugcaaccg gcgccggcug      180

gaccgccgga agcgccggau ccagcugcuc caggacaucu ucgcccccga gaucuacaag      240

aucgacccgg gguucuuccu gcgccugaag gagucccggu accuccccga ggacaagaag      300

aaccuggagg gcaagacccc cgagcugccc uacgcccucu ucguggacaa gaaguacacg      360

gacaaggacu uccacaagga guacccgacc aucuaccacc uccgccugcg gcucaugcag      420

gaccgcaccc ccgccgacgu ccggcuggug uaccuggcgc uccaccaccu gaucaagcac      480

cgcgggcacu uccuguucgc cggcaucgag gccgacaagg ugaccgacuu cagcgccgcg      540

uucuccgagu uccuccggau cguccaggag caggagcugg acaucagcuu cacgcccgac      600

gaggagucca gccagaucau caaggagacc cugcagaaca ccaacuucac ccgcuccaag      660

aaguccacgg agcucaucaa ggggcugaag gccagcaccg gcugcgagaa ggcccugcuc      720

aagcugauca gcgggugcaa ggugaagcug uccgccgugu ucgacgaccc cggcugcgac      780

gccuacgaga agccgcaggu cugcuucgcg gagagcuccu acgaggacua cgccgccauc      840

cucgaggacg agcuggagga ccgguucauc gugaucgccg ccgcgaaggc ccuguacgac      900

uggagcgugc ucgucaacau ccugggcgag gccuccaccg ugagcgaggc caaggugggg      960

aucuacgaga agcaccgccg cgaccugaag uaccucaagc accuggucaa gaccaaccug     1020

acgcgggagg acuaccagga ccucuucgug aaggccggcg cgaagaagaa caacuacugc     1080

gccuacaucg ggaugaccaa gaagaacaac aagaaggugg uccuggagga gaagcgcugc     1140

accaaggagg acuucuacgc cuaccugaag aaggagaucu acuccaaggu gcaggagaac     1200

cccgaggccg aguaccuccu ggaggagcug gagaagggca ccuuccuccc ccgccaggug     1260

agcaaggaca acggcgucau ccccuaccag cugcaguucc uggagcucca gaagauccug     1320

aagaacgccg uggaguaccu gccguuccug aaggaggaga gcgagaagau ccagcagauc     1380

augacguucc ggauccccua cuacgugggg ccccucaacg gcaagaagga ggggcagaac     1440

accuucgcgu gggccguccg gaagaacaac gagcccgugu acccguggaa cuucgaggag     1500

gucaucgaca ccgaggccuc cgccgagcag uucauccgcc ggaugaccaa caagugcacg     1560

uaccugcccg gcgaggacgu gcugcccaag gagagccucc uguacuccaa guucauggug     1620

cugaacgagc ucaacaaccu gcgcaucaac gggaccaaga ucagcgucga gcugaagcag     1680

gagcucuacg agaagcuguu ccagcgguac cgcaaggugu ccgcgaagaa gcugcgggac     1740

uaccucgugc gcaacggcau caccgagaag gacgccgaga ucagcggcau cgacggggac     1800

uucaagggca cccugcgggc cuaccacgac uucaaggaga agcugacggg ggucgagcuc     1860

accgaggccg agaaggagac caucauccug aacaucaccc uguucggcga ggacaaggug     1920

cuccugaaga agcgcaugca gcggcugcuc cccaaccuga cggaggggca gaagaaggcc     1980

aucgugaccc ugaacuacuc cggcuggggc cgccucagcc gggaguuccu ggaggagguc     2040

caggcgccga accccgaguc cggggagauc cugagcauca uccgcgcccu cugggagacc     2100

aacgccaacc ugaugcagcu gcucuccaac caguacggcu accaggagau ccuggagcag     2160

augcgggccg acuggaccgg gcacaagcug gacuaccaga cgguggagga gcucuacgug     2220

agccccgccg ucaagcgcca gaucuggcag acccuccagg uggugaagga gcugaagaag     2280

gucaugggcg gggagcccaa gcgcguguuc cucgaggugg cgcgggagaa gauggagucc     2340

ggccgcaccg ucagccggaa gaagcaccug aaggaccugu accucuccug caagagcgag     2400

gagcgcaacu ggcuggccga gcuggaccgg cacgaggagc acuccuaccg cagcgaccgg     2460

cuguuccugu acuacaccca gaagggccgc ugcauguacu gcgggaagcc gaucgaccug     2520

ggccaccucu gggacgugaa cauguacgac aucgaccaca ucuaccccca guccaaggug     2580

auggacgaca gcauccggaa ccgcguccug accugccgga cgugcaacgc cgagaagacc     2640

gaccacuacc cccugaaggc cgagauccgc cagaagaugc ggcccuucug ggacagccuc     2700

aaggccggga acuucaucga ggaggagaag uggaagcgcc ugauccgguc cgaggaguuc     2760

accagcaccg agcuggcggg cuucaucgag cgccagcucg uggagacgcg gcaguccacc     2820

aaggccgugg ccgacauccu gaagcaggcc augccggaga ccgagaucgu cuacgugaag     2880

gcgaagaacg ugagcgccuu ccgccaggac uucaacuucg ucaagaaccg ggacgugaac     2940

gacuuccacc acgccaagga cgccuaccug aacaucgugg ucggcaacac cuacuucgug     3000

aaguucacga agaacgccuc cugguucauc gagcgcaacc ccgggcggag cuacaaccuc     3060

aagcgcaugu ucaccuccga cuacgacgug augagcaacg gcgagaucgc guggaaggcc     3120

gggaacuccg gcaccaucac cacgguccgc aagaugauga agaagaacag cgugcuguuc     3180

acccggcgcu ccuucgaggu gagcgggggc cuguucgacc agcagcucau gaagaagggc     3240

aaggggcagg ucuacgugaa guccggcgac gagcggcugc gcagcaucga gaaguacggg     3300

ggcuacaaca aggccaccgg ggccuacuuc augcuggugg aguccgaggg caagaagggc     3360

gucaagaagc ggaccaucga guucgugccc cucuaccgca aggccgagcu ggaggcgagc     3420

gaggagaaga ugcuggagua ccuccggacg gagaucgggc ugaaggagcc gcacgugcug     3480

cuccacaaga ucaagaccga cacccuguuc caggccgacg gcuucaagau gcaccugucc     3540

gggcgcaccg gcaagcagcu cgucuucaag ggggccaacc aguuccuggu ggccgaggac     3600

uacgagcggc ugcucaagaa ggugggcaag gucacggagc agcugaagac caacaagaac     3660

uacaagauca acgacuacga cggcaucacc gaggaggggc ugaaggcccu cuacagcgag     3720

uuccugagca agcugcagaa ggcgcccuac ggcaaccgcc ucgggaccca ggccgagacg     3780

cuggagaacg gcaaggagaa guucgacaag cugucccucg agaacaagug ccaggugcug     3840

agcgagaucc ugcaccucuu ccagugcaac uccagcgccg ccgaccuguc caagcucggg     3900

ggccccggcc acgggggcau ccuggugcug agcaacgaga ucaccaagug caacaagauc     3960

uccaucaucc accagagccc cgcggggauc uacgagcagg agaucgaccu gaagacccuc     4020

uga                                                                   4023


<210>  1824
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1824
auggagcagg aguacuaccu gggccucgac auggggaccg gcagcguggg gugggccguc       60

accgacuccg aguaccacgu gcugcgcaag cacggcaagg cccugugggg cgugcgccuc      120

uucgagagcg cguccacggc cgaggagcgg cggauguucc gcaccagccg gcgccggcug      180

gaccgccgga acuggcgcau cgagauccug caggagaucu ucgccgagga gaucuccaag      240

aaggaccccg gguucuuccu ccggaugaag gagagcaagu acuacccgga ggacaagcgc      300

gacaucaacg gcaacugccc cgagcugccc uacgcccugu ucgucgacga cgacuucacc      360

gacaaggacu accacaagaa guuccccacc aucuaccacc uccggaagau gcugaugaac      420

acggaggaga ccccggacau ccgccuggug uaccucgcca uccaccacau gaugaagcac      480

cgggggcacu uccugcuguc cggcgacauc aacgagauca aggaguucgg gaccaccuuc      540

agcaagcucc uggagaacau caagaacgag gagcuggacu ggaaccucga gcugggcaag      600

gaggaguacg cgguggucga guccauccug aaggacaaca ugcucaaccg cagcacgaag      660

aagacccggc ugaucaaggc ccugaaggcc aaguccaucu gcgagaaggc cgugcucaac      720

cugcuggccg ggggcaccgu gaagcucagc gacaucuucg gccuggagga gcugaacgag      780

accgagcgcc ccaagaucuc cuucgcggac aacggguacg acgacuacau cggcgagguc      840

gagaacgagc ucggggagca guucuacauc aucgagacgg ccaaggccgu guacgacugg      900

gccgugcugg ucgagauccu gggcaaguac accagcaucu ccgaggcgaa gguggccacc      960

uacgagaagc acaagagcga ccuccaguuc cugaagaaga ucgugcggaa guaccugacc     1020

aaggaggagu acaaggacau cuucgucucc acgagcgaca agcucaagaa cuacuccgcc     1080

uacaucggca ugaccaagau caacgggaag aagguggacc ugcagagcaa gcgcugcagc     1140

aaggaggagu ucuacgacuu caucaagaag aacgugcuga agaagcucga gggccagccc     1200

gaguacgagu accugaagga ggagcuggag cgggagaccu uccuccccaa gcaggucaac     1260

cgcgacaacg gggugauccc guaccagauc caccuguacg agcugaagaa gauccucggc     1320

aaccugcgcg acaagaucga ccugaucaag gagaacgagg acaagcucgu gcagcuguuc     1380

gaguuccgga uccccuacua cgucgggccc cugaacaaga ucgacgacgg caaggagggc     1440

aaguucaccu gggccgugcg caaguccaac gagaagaucu accccuggaa cuucgagaac     1500

guggucgaca ucgaggccag cgcggagaag uucauccggc gcaugacgaa caagugcacc     1560

uaccugaugg gggaggacgu gcucccgaag gacucccugc uguacagcaa guacaugguc     1620

cucaacgagc ugaacaacgu gaagcuggac ggcgagaagc ucuccgugga gcugaagcag     1680

cggcuguaca ccgacgucuu cugcaaguac cgcaagguga ccgugaagaa gaucaagaac     1740

uaccucaagu gcgaggggau caucagcggc aacgucgaga ucacggggau cgacggcgac     1800

uucaaggccu cccugaccgc cuaccacgac uucaaggaga uccugaccgg caccgagcuc     1860

gccaagaagg acaaggagaa caucaucacg aacaucgugc uguucgggga cgacaagaag     1920

cugcucaaga agcggcugaa ccgccuguac ccccagauca cccccaacca gcucaagaag     1980

aucugcgccc ugagcuacac cggcuggggg cgguucucca agaaguuccu ggaggagauc     2040

accgcgcccg acccggagac gggcgaggug uggaacauca ucaccgcccu cugggagagc     2100

aacaacaacc ugaugcagcu gcucuccaac gaguaccgcu ucauggagga ggucgagacc     2160

uacaacaugg ggaagcagac caagacgcug agcuacgaga ccguggagaa cauguacgug     2220

ucccccagcg ucaagcggca gaucuggcag acccugaaga ucgugaagga gcucgagaag     2280

gugaugaagg agucccccaa gcgcgucuuc aucgagaugg cccgggagaa gcaggagagc     2340

aagcgcaccg agucccggaa gaagcagcug aucgaccugu acaaggccug caagaacgag     2400

gagaaggacu gggugaagga gcucggcgac caggaggagc agaagcugcg cagcgacaag     2460

cuguaccucu acuacaccca gaagggccgg ugcauguacu ccggggaggu gaucgagcug     2520

aaggaccugu gggacaacac gaaguacgac aucgaccaca ucuaccccca gagcaagacc     2580

auggacgaca gccucaacaa ccgcguccug gugaagaaga aguacaacgc caccaagucc     2640

gacaaguacc cgcugaacga gaacauccgg cacgagcgca agggcuucug gaagucccuc     2700

cuggacgggg gcuucaucag caaggagaag uacgagcgcc ugauccggaa caccgagcuc     2760

agccccgagg agcuggcggg cuucaucgag cgccagaucg uggagacgcg gcaguccacc     2820

aaggccgucg ccgagauccu gaagcagguc uuccccgaga gcgagaucgu guacgugaag     2880

gccgggaccg ugucccgcuu ccggaaggac uucgagcucc ugaaggugcg cgaggucaac     2940

gaccugcacc acgcgaagga cgccuaccuc aacaucgugg ugggcaacag cuacuacguc     3000

aaguucacca agaacgccuc cugguucauc aaggagaacc ccgggcggac guacaaccug     3060

aagaagaugu ucaccagcgg cuggaacauc gagcgcaacg gggagguggc cugggaggug     3120

ggcaagaagg gcaccaucgu caccgugaag cagaucauga acaagaacaa cauccuggug     3180

acgcggcagg uccacgaggc caagggcggc cucuucgacc agcagaucau gaagaagggg     3240

aaggggcaga ucgcgaucaa ggagaccgac gagcgccugg ccuccaucga gaaguacggg     3300

ggcuacaaca aggccgccgg cgccuacuuc augcuggugg agagcaagga caagaagggg     3360

aagaccaucc ggaccaucga guucaucccg cucuaccuga agaacaagau cgaguccgac     3420

gagagcaucg cgcugaacuu ccucgagaag ggccgcgggc ugaaggagcc caagauccug     3480

cucaagaaga ucaagaucga cacgcuguuc gacguggacg gcuucaagau guggcugucc     3540

gggcggaccg gcgaccgccu ccuguucaag ugcgccaacc agcugauccu cgacgagaag     3600

aucaucguca ccaugaagaa gaucgugaag uucauccagc ggcgccagga gaaccgggag     3660

cugaagcuga gcgacaagga cggcaucgac aacgaggugc ucauggagau cuacaacacc     3720

uucgucgaca agcuggagaa cacgguguac cgcauccggc uguccgagca ggccaagacc     3780

cucaucgaca agcagaagga guucgagcgc cugagccugg aggacaagag cuccacccuc     3840

uucgagaucc ugcacaucuu ccagugccag agcuccgccg ccaaccugaa gaugaucggg     3900

ggccccggga aggcgggcau ccucgugaug aacaacaaca ucagcaagug caacaagauc     3960

uccaucauca accagagccc caccgggauc uucgagaacg agaucgaccu gcugaagauc     4020

uga                                                                   4023


<210>  1825
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1825
augggcgagg aguacuuccu ggggcucgac augggcaccg ggagccuggg cugggccgug       60

accgacgaca aguaccagau ccugcgccgg cacgggaacg cccucugggg cguccgccug      120

uucgagacgg cgaacaccgc cgaggagcgg cgcauguucc ggaccaaccg ccggcgccug      180

gaccggcagg gcuggcgcau caagauccuc caggagcugu ucgccgagga gaucugccgg      240

guggaccccg gguucuaccu gcgcaugaag gaguccaagu acuacccgga ggacaagcgg      300

gacgccaacg gcgagugccc cgcccucccc uacgcgcugu ucgccgacgc ccaguacacc      360

gacaaggacu accaccgcga guuccccacg aucuaccacc uccggaagau gcugauggag      420

accgagacca ccccggacau ccgccuggug uaccuggccu uccaccacau gaugaagcac      480

cgggggcacu uccuccugag cggcgacauc ucccagguca cggaguuccg caacaccuuc      540

cugcagauga ucgcgaacau caagagcgag gagcucgacu ucucccugga gaucgaggag      600

acccagaugg ggcagaucga ggccgugcug aaggaccaca agcucacccg gagcgagaag      660

aagcgccggc ugacgcagau ccugaagccc gccaccgccu gcgagaaggc caucuucacc      720

cuccuguccg gcggcaccgu gaagcugagc gacaucuucg ggaaggcgga gcucgacgag      780

ggcgagcgcc ccaagaucuc cuucgccgac aacggguacg acgacuacag cgccuccguc      840

gaggccgacc ugggcgagca guacuacauc aucgagagcg ccaaggcggu guacgacugg      900

uccgugcuga gcgacauccu cggcgacucc gucagcaucu ccgacgccaa ggugaagcug      960

uacgagaagc acaagaagga ccuggccuac cucaagaagg ugguccgcga gaaccugccc     1020

cgggaggugu acaaccaggu guucgucaac acggacgaga agcugugcaa cuacugcgcc     1080

uacaucggga uggccaagaa gaacggccgc aagguggcgc ucgaggggaa gcggugcacc     1140

cgcgaggagu ucgagagcuu ccugcggaag aagaucguga acaagaucga ggacaaggag     1200

auccaggagu accuguccga ggagaucgag aagggcaccu uccucccgcg ccaggucacc     1260

aaggacaacg gggugauccc cuaccagauc caccuguacg agcugcggaa gaucaucgac     1320

aaccucagcc cccgcaugcc cuucauccgg gacaacggcg acaagaucau ccagcuguuc     1380

acguuccgca ucccguacua cgugggcccc cuggggaagg gcgaggggag cgagaaguuc     1440

uccugggccg uccggcgcac cgacgaggcc aucuaccccu ggaacuucaa ggacgugauc     1500

gacguggagg ccagcgccga gaaguucauc ugccggauga ccaacaagug caccuaccuc     1560

uacggcgagg acguccugcc caaggacucc ccgcuguaca gcaaguucau ggugcucaac     1620

gagcugaaca accugaagcu ggacgggcag uccgucagcg uggagcucaa gcagcgcauc     1680

uaccaggagc uguucugcca cacgcggaag gugacccaga agaagcuggu cuccuaccuc     1740

gugcgcgagg gcaucaccgg caagaacgug gagaucagcg ggaucgacgg cgacuucaag     1800

gcgucccuga ccgccuacca cgacuucaag gagaagcuga cgggggucga gcucagccag     1860

caggagcagg aggagaucgu gcggaacauc gugcuguucg gcgaggacaa gaagcugcuc     1920

aagcagcgcc uggaggggcg guucccccag cugacccaga agcagaucuc cgucaucgcc     1980

acccucagcu acaagggcug gggccgccug ucccgcgagu uccuggagaa caucaccgug     2040

cccgcccccg ggacgggcga gguguggaac aucaucaccg cgcucuggga gaccaacgac     2100

aaccugaugc agcugcucua cggggaguac ggcuuccaga ccgagaucga gugcuucaac     2160

gccaaggaga agaagaaguc ccugagcuac gagacgaucg aggagagcua cgucuccccg     2220

agcgugaagc ggcagaucug gcagacccuc caggugguca aggagcugaa gaaggugaug     2280

cgcggcgacc ccaagcgggu guucaucgag auggcccgcg agaaccagga guccaagcgg     2340

accgagagcc gcaagaagac ccugcuggac cucuacaaga acugcaagaa ggaggagccc     2400

gaguggaucg ccgagcugac cgagcaccug gagaagcucg acgacagcca gcugcggaag     2460

gacaagcugu uccucuacua cacgcagaag gggcgcugca uguacuccgg cgagcggauc     2520

gagcuggagg accuguggga caacacccgc uacgacaucg accacaucua cccccagagc     2580

aagaccaugg acgacuccgu cgacaaccgg gugcucguga agaaggagua caacgccaag     2640

aagaccgacg ucuacccgau cgcggccgac auccgcggga agaugagccc cuucuggaag     2700

aagcugcugg acaacaagcu caucuccaag aagaaguacg accggcuggu gcgcacggag     2760

gagcugaccc ccgaggagcu cgccggcuuc aucgaccggc agcuggugga gacccgccag     2820

agcaccaagg ucguggccga gauccugaag gaggccuucc ccgacacgga gaucguguac     2880

gucaaggcgg ggaacguguc caacuuccgg cacauguucg acaucgugaa gguccgcgag     2940

cucaacgacc ugcaccacgc caaggacgcc uaccugaaca ucgugguggg caacgccuac     3000

uacgucaagu ucaacaagaa cgccgcgugg uucaucaagg agaacccggg ccggagcuac     3060

aaccucaagc gcauguucga cuacgacauc cggcgcaaca acgagguggc cuggcuggcc     3120

ggguccaccg gcaccaucag ccaggugaag aacauggucu ccaagaacca cauccuggug     3180

acccgcaaga gcuacgaggu gaccgggggc cucuucgacc agcagcugau gaagaagggg     3240

aagggccagg uccccgugaa gacguccgac gagcggcugg gcaacaucga gaaguacggg     3300

ggcuacaaca aggccagcgg ggcguacuuc augcucguga aguccaagaa caagaagggc     3360

gaggagcagc gcaccaucga guacgucccc aucuaccuga agcacuacau cgaggagagc     3420

aaggagaacg cccuggacua ccucaagaac gagcggaagc ugaacgagcc ggagauccug     3480

cucgacaaga ucaagaagga cacccuguuc cgcgugaacg gguucuacau gugccugucc     3540

aagcggacgg gcaagcagcu cgccuucaag ggcgccaacg agcugcugcu ccccgagccc     3600

gagacccugc ugcucaagaa gaucaccaag uacauccagc gccggaagga gaacaaggac     3660

cugaagcuga ccaagcagga caacaucacg gagaaggagc uccuggagcu guacgacgug     3720

uucaccgaga agcucaacca cagcgucuac gccgugugcc uggggaccca gggcaagacc     3780

cuggugcaga agaagcccgc guucgucgcc cucccggugg aggagaagug caucgugcug     3840

agcgagaucc ugcaccucuu ccagugccag uccgggagcg ccaaccugaa gcugaucggc     3900

gggcccgccu ccgccggcau ccucuaccug aacagcaaca ucuccgacgc gggcgagauc     3960

cugaucauca accagagccc cuccgggauc uucgagaagc gcaucgaccu ccugcggcug     4020

uga                                                                   4023


<210>  1826
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1826
augaagaagg aguacaccau cggccuggac aucgggacca acagcguggg cugguccguc       60

cucacggacg acuaccgccu ggugagcaag aagaugaagg uggccgggaa caccgagaag      120

uccagcacca agaagaacuu cuggggcguc cggcuguucg acgaggggca gaccgccgag      180

gcgcgccggu ccaagcgcac ggcccggcgc cggcucgccc gccggcgcca gcggauccug      240

gagcugcaga agaucuucgc ccccgagauc cucaagaucg acgagcacuu cuucgcccgc      300

cugaacgaga gcuuccuggu gccggacgag aagaagcagu cccggcaccc cguguucgcg      360

accaucaagc aggagaaguc cuaccaccag accuacccca ccaucuacca ccuccgccag      420

gcccuggccg acagcagcga gaaggccgac auccggcugg ucuaccucgc gauggcccac      480

cuccugaagu accgcggcca cuuccugauc gagggcgagc ugaacacgga gaacuccagc      540

gugaccgaga ccuuccggca guuccugucc accuacaacc agcaguucag cgaggccggg      600

gacaagcaga cggagaagcu cgacgaggcc guggacugcu ccuucgucuu caccgagaag      660

augagcaaga ccaagaaggc cgagacccug cugaaguacu ucccccacga gaaguccaac      720

ggcuaccuca gccaguucau caagcugaug guggggaacc agggcaacuu caagaacgug      780

uucgggcugg aggaggaggc gaagcuccag uucuccaagg agacguacga ggaggaccug      840

gaggagcugc ucgagaagau cggcgacgac uacaucgacc uguucgucca ggccaagaac      900

guguacgacg ccgugcugcu cagcgagauc cugagcgacu ccaccaagaa cacccgcgcc      960

aagcugagcg ccggcaugau ccggcgcuac gacgcgcaca aggaggaccu cguccugcug     1020

aagcgguucg ugaaggagaa ccucccgaag aaguaccgcg ccuucuucgg ggacaacucc     1080

gugaacggcu acgccgggua caucgagggc cacgccaccc aggaggccuu cuacaaguuc     1140

gucaagaagg agcugacggg cauccgcggg agcgaggugu uccugaccaa gaucgagcag     1200

gagaacuucc uccggaagca gcgcaccuuc gacaacggcg ugauccccca ccagauccac     1260

cuguccgagc ugcgggcgau caucgccaac cagaagaagc acuaccccuu ccucaaggag     1320

gagcaggaga agcuggagag ccugcucacc uucaagaucc ccuacuacgu cgggccgcug     1380

gccaagaagc aggagaacuc ccccuucgcc uggcugaucc gcaagagcga ggagaagauc     1440

aagcccugga accuccccga gaucguggac auggagggcu ccgccgugcg guucaucgag     1500

cgcaugauca acacggacau guacaugccg cacaacaagg uccugcccaa gaacagccug     1560

cuguaccaga aguucuccau cuacaacgag cucaccaagg ugcgguacca ggacgagcgc     1620

gggcagauga acuacuucag cuccaucgag aagaaggaga ucuuccacga gcuguucgag     1680

aagaaccgga aggucaccaa gaaggaccug caggaguucc ucuaccugaa guacgacauc     1740

aagcacgcgg agcugagcgg caucgagaag gccuucaacg ccuccuacac cacguaccac     1800

gacuuccuca ccaugagcga gaacaagcgc gagaugaagc aguggcugga ggaccccgag     1860

cuggccucca uguucgagga gaucaucaag acccucaccg uguucgagga ccgggagaug     1920

aucaagacgc gccugagcca ccacgaggcg acccugggca agcacaucau caagaagcuc     1980

accaagaagc acuacaccgg guggggccgg cuguccaagg agcugaucca ggggauccgc     2040

gacaagcaga gcaacaagac gauccucgac uaccugauca acgacgacga cuucccccac     2100

caccggaacc gcaacuucau gcagcugauc aacgacgaca gccucuccuu caagaaggag     2160

aucaagaagg cccagaugau caccgacacc gagaaccugg aggagaucgu gaaggagcug     2220

accggcagcc cggccaucaa gaaggggauc cuccaguccc ugaagaucgu cgacgagauc     2280

gugggcauca ugggcuacga gcccgccaac aucguggucg agauggcccg ggagaaccag     2340

accacggggc gcggccucaa gagcucccgg ccccgccuga aggcgcugga ggagagccug     2400

aaggacuucg ggucccagcu ccugaaggag uaccccaccg acaacagcuc ccugcagaag     2460

gaccgccucu accuguacua ccugcagaac ggccgggaca uguacaccgg ggccccgcuc     2520

gacauccacc gccugagcga cuacgacauc gaccacauca ucccccgguc cuucaccacg     2580

gacaacagca ucgacaacaa ggugcuggug uccagcaagg agaaccgccu caagaaggac     2640

gacguccccu ccgagaaggu ggugaagaag augcggagcu ucugguacga ccuguacucc     2700

agcaagcuga ucagcaagcg caagcucgac aaccugacca agaucaagcu gaccgaggag     2760

gacaaggccg gcuucaucaa gcggcagcuc gucgagaccc gccagaucac gaagcacgug     2820

gccggcaucc ugcaccaccg guucaacaag gccgaggaca ccaacgaccc cauccgcaag     2880

gugcggauca ucacccugaa guccgcgcuc gucagccagu uccgcaaccg guucgggauc     2940

uacaaggugc gcgagaucaa cgaguaccac cacgcccacg acgccuaccu gaacggcgug     3000

gucgcccugg cccuccugaa gaaguacccg cagcuggcgc ccgaguucgu guacggggag     3060

uaccucaagu ucaacgccca caaggccaac aaggccaccg ugaagaagga guucuacucc     3120

aacaucauga aguucuucga gagcgacacg cccgucugcg acgagaacgg cgagaucuuc     3180

ugggacaagu ccaagagcau cgcccaggug aagaagguga ucaaccacca ccacaugaac     3240

aucgucaaga agaccgagau ccagaagggc ggguucucca aggagaccgu ggagcccaag     3300

aaggacagcu ccaagcugcu gccgcggaag aacaacuggg accccgcgaa guacggcggg     3360

cucggcagcc ccaacguggc cuacaccguc gccuucacgu acgagaaggg gaaggcccgc     3420

aagcggacca acgcgcugga gggcaucacc aucauggagc gcgaggccuu cgagcagucc     3480

ccggugcugu uccucaagaa caagggcuac gagcaggccg agaucgagau gaagcugccc     3540

aaguacgccc uguucgagcu cgagaacggg cggaagcgca ugguggccag caacaaggag     3600

gcgcagaagg ccaacuccuu ccugcugccc gagcaccucg ucacccugcu guaccacgcc     3660

aagcaguacg acgagaucag ccacaaggag uccuucgacu acgugaacga gcaccacaag     3720

gaguucagcg agguguucgc ccggguccuc gaguucgccg gcaaguacac gcuggcggag     3780

aagaacaucg agaagcugga gaagaucuac aaggagaacc agaccgacga ccucgccaag     3840

cuggccucca gcuucgugaa ccugaugcag uucaacgcca ugggggcgcc cgccgacuuc     3900

aaguucuucg acgugaccau cccgcgcaag cgcuacaccu cccucacgga gaucuggcag     3960

agcaccauca uccaccagag caucaccggc cuguacgaga cccggauccg cauggggaag     4020

uga                                                                   4023


<210>  1827
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1827
auggagcagg aguacuaccu gggccucgac auggggaccg gcagcguggg gugggccguc       60

accgacuccg aguaccacgu gcugcgcaag cacggcaagg cccugugggg cgugcgccuc      120

uucgagagcg cguccacggc cgaggagcgg cggauguucc gcaccagccg gcgccggcug      180

gaccgccgga acuggcgcau cgagauccug caggagaucu ucgccgagga gaucuccaag      240

aaggaccccg gguucuuccu ccggaugaag gagagcaagu acuacccgga ggacaagcgc      300

gacaucaacg gcaacugccc cgagcugccc uacgcccugu ucgucgacga cgacuucacc      360

gacaaggacu accacaagaa guuccccacc aucuaccacc uccggaagau gcugaugaac      420

acggaggaga ccccggacau ccgccuggug uaccucgcca uccaccacau gaugaagcac      480

cgggggcacu uccugcuguc cggcgacauc aacgagauca aggaguucgg gaccaccuuc      540

agcaagcucc uggagaacau caagaacgag gagcuggacu ggaaccucga gcugggcaag      600

gaggaguacg cgguggucga guccauccug aaggacaaca ugcucaaccg cagcacgaag      660

aagacccggc ugaucaaggc ccugaaggcc aaguccaucu gcgagaaggc cgugcucaac      720

cugcuggccg ggggcaccgu gaagcucagc gacaucuucg gccuggagga gcugaacgag      780

accgagcgcc ccaagaucuc cuucgcggac aacggguacg acgacuacau cggcgagguc      840

gagaacgagc ucggggagca guucuacauc aucgagacgg ccaaggccgu guacgacugg      900

gccgugcugg ucgagauccu gggcaaguac accagcaucu ccgaggcgaa gguggccacc      960

uacgagaagc acaagagcga ccuccaguuc cugaagaaga ucgugcggaa guaccugacc     1020

aaggaggagu acaaggacau cuucgucucc acgagcgaca agcucaagaa cuacuccgcc     1080

uacaucggca ugaccaagau caacgggaag aagguggacc ugcagagcaa gcgcugcagc     1140

aaggaggagu ucuacgacuu caucaagaag aacgugcuga agaagcucga gggccagccc     1200

gaguacgagu accugaagga ggagcuggag cgggagaccu uccuccccaa gcaggucaac     1260

cgcgacaacg gggugauccc guaccagauc caccuguacg agcugaagaa gauccucggc     1320

aaccugcgcg acaagaucga ccugaucaag gagaacgagg acaagcucgu gcagcuguuc     1380

gaguuccgga uccccuacua cgucgggccc cugaacaaga ucgacgacgg caaggagggc     1440

aaguucaccu gggccgugcg caaguccaac gagaagaucu accccuggaa cuucgagaac     1500

guggucgaca ucgaggccag cgcggagaag uucauccggc gcaugacgaa caagugcacc     1560

uaccugaugg gggaggacgu gcucccgaag gacucccugc uguacagcaa guacaugguc     1620

cucaacgagc ugaacaacgu gaagcuggac ggcgagaagc ucuccgugga gcugaagcag     1680

cggcuguaca ccgacgucuu cugcaaguac cgcaagguga ccgugaagaa gaucaagaac     1740

uaccucaagu gcgaggggau caucagcggc aacgucgaga ucacggggau cgacggcgac     1800

uucaaggccu cccugaccgc cuaccacgac uucaaggaga uccugaccgg caccgagcuc     1860

gccaagaagg acaaggagaa caucaucacg aacaucgugc uguucgggga cgacaagaag     1920

cugcucaaga agcggcugaa ccgccuguac ccccagauca cccccaacca gcucaagaag     1980

aucugcgccc ugagcuacac cggcuggggg cgguucucca agaaguuccu ggaggagauc     2040

accgcgcccg acccggagac gggcgaggug uggaacauca ucaccgcccu cugggagagc     2100

aacaacaacc ugaugcagcu gcucuccaac gaguaccgcu ucauggagga ggucgagacc     2160

uacaacaugg ggaagcagac caagacgcug agcuacgaga ccguggagaa cauguacgug     2220

ucccccagcg ucaagcggca gaucuggcag acccugaaga ucgugaagga gcucgagaag     2280

gugaugaagg agucccccaa gcgcgucuuc aucgagaugg cccgggagaa gcaggagagc     2340

aagcgcaccg agucccggaa gaagcagcug aucgaccugu acaaggccug caagaacgag     2400

gagaaggacu gggugaagga gcucggcgac caggaggagc agaagcugcg cagcgacaag     2460

cuguaccucu acuacaccca gaagggccgg ugcauguacu ccggggaggu gaucgagcug     2520

aaggaccugu gggacaacac gaaguacgac aucgaccaca ucuaccccca gagcaagacc     2580

auggacgaca gccucaacaa ccgcguccug gugaagaaga aguacaacgc caucaagucc     2640

gacaaguacc cgcugaacga gaacauccgg cacgagcgca agggcuucug gaagucccuc     2700

cuggacgggg gcuucaucag caaggagaag uacgagcgcc ugauccggaa caccgagcuc     2760

agccccgagg agcuggcggg cuucaucgag cgccagaucg uggagacccg gcaguccacg     2820

aaggccgucg ccgagauccu gaagcagguc uuccccgaga gcgagaucgu guacgugaag     2880

gccgggaccg ugucccgcuu ccggaaggac uucgagcucc ugaaggugcg cgaggucaac     2940

gaccugcacc acgcgaagga cgccuaccuc aacaucgugg ugggcaacag cuacuacguc     3000

aaguucacca agaacgccuc cugguucauc aaggagaacc ccgggcggac cuacaaccug     3060

aagaagaugu ucacgagcgg cuggaacauc gagcgcaacg gggagguggc cugggaggug     3120

ggcaagaagg gcaccaucgu caccgugaag cagaucauga acaagaacaa cauccuggug     3180

acccggcagg uccacgaggc caagggcggc cucuucgacc agcagaucau gaagaagggg     3240

aaggggcaga ucgcgaucaa ggagacggac gagcgccugg ccuccaucga gaaguacggg     3300

ggcuacaaca aggccgccgg cgccuacuuc augcuggugg agagcaagga caagaagggg     3360

aagaccaucc ggaccaucga guucaucccg cucuaccuga agaacaagau cgaguccgac     3420

gagagcaucg cgcugaacuu ccucgagaag ggccgcgggc ugaaggagcc caagauccug     3480

cucaagaaga ucaagaucga cacccuguuc gacguggacg gcuucaagau guggcugucc     3540

gggcggacgg gcgaccgccu ccuguucaag ugcgccaacc agcugauccu cgacgagaag     3600

aucaucguca ccaugaagaa gaucgugaag uucauccagc ggcgccagga gaaccgggag     3660

cugaagcuga gcgacaagga cggcaucgac aacgaggugc ucauggagau cuacaacacc     3720

uucgucgaca agcuggagaa caccguguac cgcauccggc uguccgagca ggccaagacg     3780

cucaucgacc gccagaagga guucgagcgg cugagccugg aggacaagag cuccacccuc     3840

uucgagaucc ugcacaucuu ccagugccag agcuccgccg ccaaccugaa gaugaucggg     3900

ggccccggga aggcgggcau ccucgugaug aacaacaaca ucagcaagug caacaagauc     3960

uccaucauca accagagccc caccgggauc uucgagaacg agaucgaccu gcugaagauc     4020

uga                                                                   4023


<210>  1828
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1828
augcgcuucc agaacaagaa gaccggcgac uacuucgugg ggcuggacau cggcaccaac       60

agcgucgggu gggccgugac gaacgagaag uacgagaucc uccgggccaa gaaccaccgc      120

auguggggcu cccggcuguu cgaggagggg aagaccgcgg aggagcgccg ggugcaccgc      180

gccggccggc gccggcugaa ccgccggaac gaccgccuca ugauccugga ggaccuguuc      240

gccgaggaga ucagcaaggu cgacaaggcc uucuaccagc ggcuccacga gucccaguac      300

cgccaggagc acaagaccgu gagcggcccc uacuuccugu ucaacgacaa ggaguucacc      360

gacgaggacu accacaucga cuacccgacg aucuaccacc uccggguggc ccugaugaag      420

gcgcccgcca aggacguccg ccugcuguuc cucgccaucc accacaucau caaguaccgg      480

gggaacuucc ugcacgaggg cgagaccuuc aagaccaccg ggggccugac ggacauccuc      540

cagcgcgugc uggccugcaa cgaguucucc gggcugaagc agcacaucga cgacaccgcg      600

cucgugaagc ugcugaccaa caagggcauc acccggagcg acaucgccaa gcagaucucc      660

gagcucgcca cggacaagcu guaccgcaag caggccaagg ccuucgcgag ccugguccuc      720

ggccugaagg ccaaccugga cgaccucuuc aacgagcccg aguacaagga gcuggacucc      780

gagauccgga ccguggaguu cagcgggaag gaguacuggg aggagcgcga caaguacgag      840

cccuaccugg gcgagucccu cuucgcccug gacgggugca agacccugua cgacacccuc      900

cugcugagca acaucaagcc ggacggcaag uaccucuccg aggccaaggu ggagacguuc      960

gagaagcacc acgagcaccu ggagagccug aaggaccucc ugaaguccaa caaggcccug     1020

uacaaccgcg uccuccggga cgacgagaag aaccugaaca acuacaucac cuacaucggc     1080

aagagcgagu ucaacaagcg cugcucccgg gacgaguucu acaaguuccu gaagcagacc     1140

aucaaggaca ucagcgacaa cgagaacaag gcggagaucg ugcagcucau ggagcuggac     1200

uccuuccugc cccuccugcg cgugcgggag aacgggguca uccccuacca gauccacgag     1260

gaggagcugg agcagauccu gaacgugagc agcgagaagu accccuuccu caacaaggcc     1320

gacggcuccg gguucaccau cgcccagaag auccugaagc ugcucacguu ccgcaucccg     1380

uacuucgugg gcccccugaa ccccgcccac aaggaccggg acaacggguu cgccuggguc     1440

gugcgcaagg cgcccggcaa gguguacccg uggaacuucg aggacaaggu cgacaucagc     1500

gccuccgcca ccgccuucau cgagaaccug accaacaagu gcaccuaccu caucggcgag     1560

gacgugcugc ccaaguacag ccuggccuac uccaaguuca gccuccugaa cgagcugaac     1620

aaccuccggu acgacgggaa gccccugucc uacgagacga agcagagcuu caucaaggac     1680

ugcuucgaga aggagcaccg caagaugacc gagaagcgga uccgcgaguu ccugaagcac     1740

cucggcggga ccgacggcaa gggggcgcug accggccugg acguggacau caaguccgac     1800

cucaagagcu accgggacau gguccgcauc cugggcgacg gguucgacga gacgauggcc     1860

gagcggauca uccgcuacgu gacccuguuc ggcgaggcca agaagguccu ccuguccacc     1920

cuggagaucg aguucgggga cgaccucagc gacacccaga agaagcagau ccugaagcug     1980

caguaccggg acuggggccg ccucuccgcc acgcugcuga aggggaucaa gggcguggcg     2040

ggcgacaacc ccgagugcag cauccucgag ugcauggagc agcagccgcu gaaccugaug     2100

gaggugcucu ccagccacaa caccuaccug gccgccaucg aggaguucaa caaggagcgg     2160

accgagccca ccgacaucgu cgacuacaag cugguggacg accucuacau cucccccgcc     2220

gugaagcgca gcgucuggca gacgcugcgc auccuggacg agaucaccaa gcuccggggg     2280

uccgugccca agaagguguu cgucgaggug gcccgcacca accgggcgga gaagaagaag     2340

accgacagcc gcaaggagcg gcugcugcag cucuacaaga ccaucaagga cgagggccgc     2400

aacuggacgg aggagaucaa gcagcgggac gccggggacu ucaacagcaa gaagcuguuc     2460

cuguacuaca cccagcaggg ccgcugcaug uacuccggcg agaagaucac cagcaucggg     2520

gaccucuuca ccuccaagua cgacaucgac cacaucuacc cgcggagcgu gacgaaggac     2580

aacuccuggg acaaccuggu ccuggugaag gccgagcuca accgcgagaa gaaggacauc     2640

uaccccaucg agaaggacac ccaggaccgg ugcaucagcc uguggaagca gcugcucgag     2700

cagggccuga ucuccgagcg caaguacgag cggcuggugc gcaccacgcc ccucaccgcc     2760

gacgagcugg ccgacuucgu caaccggcag cugguggaga ccaaccagag cgugaaggcg     2820

gucaccaacc uccugaagcg ccuguacccc gacaccacga ucugcuacgu gaaggccgag     2880

aacguguccg acuuccggca ggagcgcgag uucgucaagc uccggagccu gaacgcccac     2940

caccacgcca aggacgccua ccugaacauc guggugggga acgucuacca cgagaaguuc     3000

accaacaacc cgcgcaacuu caucaagaac gcgcgguccg acaagaagcg ccccuacagc     3060

cucaaccaca uguucgacua ccugcuccag gugggcaaca aggagaucug gaaccccaag     3120

accgacaaca accuggugaa gaagaugaug cgcaacaacg acguccaggu gacccggaug     3180

cugguggagc agacgggggc ccuguacgac gccaccaucu acaaggccuc cgucaccaag     3240

agcgaguccu acgugccccu caagagcucc gacgcgaucc ugagcgacgu gaagaaguac     3300

ggcggcuuca ccagcaucaa gaucgccuac uacuccaucu uccgcuucua caagaagaac     3360

agcgaggagu ucgagacgca caucaucucc aucccggucc ugaaggccgg gacccucaac     3420

agcaaggagg acuacguggc cuacggcgag gagaucauga acaagcagaa guucgaccgg     3480

guggagcugg ucuacaagaa gcugugcaug aacucccucg ugcgcaucaa cggggugaug     3540

uacuaccugg gcgggaagac caacaacagc cuguacaucg acuccgccau cagccucguc     3600

cugcccgaca acaucgcgaa gaagaucaag gccguggaga aguacaucgc ccggguggcc     3660

gagaacggca acuacaagcc ccagccggac caggucuccc ccgaguucaa caugaucauc     3720

uacaaggccc ugaccgagaa gaugaagcag cccgcguucg ugggccucaa gacgaaccgc     3780

gagaacgagc uggagaagga gaccaccauc accgccuuca ucgccaugac gcugaucgag     3840

cagugcaaga ugcucaugga gauccugaac cugcucaccc agcagaagac caccuacgcc     3900

cugaagccca ucggggugag cgccucccgg agcacgcugg gcaugaacau caccgggcuc     3960

uccgaguuca ccaucaucac ccagagcguc acgggccugc aggagaagga gauccgcauc     4020

aucuga                                                                4026


<210>  1829
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1829
auguggggca gccgccuguu cgaggaggcc uccaccgccc aggaccggcg cgagcaccgg       60

agcaaccgcc ggcgccucga gcggcgcaag uaccggcugg accugcucga ggagcuguuc      120

guggaggaga ucaacaagau cgaccccgag uucuucaucc gccugcggga guccaaguuc      180

cacuucgagg accgcagccu caagaacacc uucauccugu ucaacgacaa gauggagggc      240

ggguacaacg acaaggucuu cuucuccaag uacccgacga ucuaccaccu ccggagcgcg      300

cucaucaacg ggaacaagga ggacgugcgc gccguguucc uggccaucca caacauccug      360

aagaaccggg gccacuuccu ccuggccggg aacgacauca acaucucccu gagccucaac      420

gacacccuga ccgaccuguu cgaguaccuc aagauccacc ugggcaucga ggucgaguuc      480

gagaacaagc ugaaggacau cgagaagauc gcccucgaca agaaccuguc caagagcgac      540

aagcagaaga agaucgugca gcugcucgag accgacuaca agaagcagcu ggcggacgug      600

uucaagcugu cccucggccu gacggccaac auggagaaca ucuucgggaa ggagaacgag      660

cagggccugg aguucaucaa cgccgagaac aagaucaaga agauccaguu cgacagcucc      720

uacgaggaga acaucgccga guucgaccag uucuacgggg aguacuucga gaucaucgac      780

aucugcaaga agaucuacga cgcgaucagc cucucccaga uccuggucga cggcaagggg      840

cugagcgagu ccaaggugcu cagcuacgag aagcacaagg aggaccugaa gcugcucaag      900

aaggugcuga agaacaucga caaggccuac gacaacaagg gcgaggaccg ccuggucuac      960

aaggugcuca aggaggacaa ggacaagggc accaacuacg ugaacuacuc caagcugagc     1020

aacaacggga ccgccaccac gaaggaggag uucuacaagu uccugagcaa gaaccucgac     1080

cugaagaagu ucggcaagcu gaacaaggag gacaucgaga cccucgacua caucaacucc     1140

gagauccugc uggggcgguu ccucccccug cagaaggaga agaucaacgg cgucaucccc     1200

uaccagcugc agaugaagga gcugacccag auccucaaca acgccagcaa guaccugccc     1260

uuccugaaca agaucgagga cggcaugacc gugaaggaca agaucaucuc ccucaucaag     1320

uuccgcaucc cguacuacgu ggggccccug aacgaguaca accggaucga gaacggcaac     1380

ggguucgccu ggaucgagaa gaagaagaac gagaagaucu uccccuggaa cuucaacgac     1440

gucguggaca ucgagaaguc cgcggagaag uucaucaaca accugacgaa caagugcacc     1500

uacaucaagg aggagaccgu gcuccccaag aacagcauca ucuacagcaa guucaugguc     1560

cugaacgagc ugaacaaccu ccaguacggc aacaagaagc uggacaucga gcugaagaug     1620

aagauguaca ccgaccucuu ccuggagaag aaggacaacc gcaaggugac ggagaagcgg     1680

auccuggagu acuuccgcau caacgggauc aacgacggca aggagaagcu caccggccug     1740

aacaacgaga uccacgccga caugaagucc uaccgcgaca ugugcgagau caugggggag     1800

aacuucaaca ucaacguggc cgagaacauc aucaacugga ucaccgucuu ccacggcgag     1860

aagaagaucc ugaucaacaa gaucaagaac guguacggga agauguucaa cgacgagcag     1920

aucagcaagc ucuccaagcu gaacuacagc gacuggggcc ggcuguccaa gaagcuccug     1980

accgacauca agugcuacau cgacgccgac gggaucaacu ucccggugag cauccuggag     2040

gccauggagg ucuccaacaa gaaccucaug gagcugcuga gcaacaagua cgaguucauc     2100

uccugcaucg acgacaucaa caacaaguac uucggcgacc gcgacaucag cgcguccgag     2160

cucaucgacg agcuguacgu gagccccgcc gucaagcggg gcaucuggca guccgugcgc     2220

auccuggagg agaucgugag caucaucggg cacaacccca agaagaucuu caucgagaug     2280

acgcggcaca acgaggagaa gguccagaag uccagccgcc ggcacaaccu cgaggagcug     2340

uacaaggcca uccgcaagga ccccgaguac guggagcuga ucaaggaguu cgacagcacc     2400

accgacaccg acuccuucaa gaucaagaag cucuaccugu acuucaugca gcugggcaag     2460

ugcgccuaca gcaaggagaa gaucgacuuc gacaagcugu ucacggacuc cuacgacauc     2520

gaccacaucu acccgcggag caagaccaag gacgacucca uccacaacaa ccuggugcug     2580

gucaaggcca aguacaaccg cgagaagacc gacguguacc ccaucgacau caacauccag     2640

aagaacauga acagcuucug gaacuuccuc uacaccaaga aguucaucac ggaggagaag     2700

uacaagcggc ugauccgcac cuccgaccug accgacgagg agcucgcgaa guucaucagc     2760

cggcagcugg uggagaccuc ccagagcacg aaggccaccg ccgagguccu gaagcagauc     2820

uacggggacu ccgacaucug cuacgugaag gccgagaacg ugagcgacuu ccgcuacggc     2880

uccagccggc agaaggacaa gaacgacauc cagaagcugu ucgucaagug ccgcgaggug     2940

aaccacuucc accacgccaa ggacgcguac cugaacaucg uggucgggaa cguguacgac     3000

gugaaguuca ccaagaaccc cgucaacuuc aucaagaaga acaagauggg caacaagucc     3060

aacuacagcc ugaaccacau guucugcuuc gacguggagc ggaacggcua caaggccugg     3120

gacagcuccg agaccaucaa gacggugggg cgcaugauga aguccaacga cguccggauc     3180

accaagcacg ugcaccccac ccgcggcgag cucuacgacg ccaccaucua caaggccaag     3240

aucgcgaaga ccgggagcua caugccgcuc aagagcucca agaacacgcc ccugagcgac     3300

gugaccaagu acggcggcua caccaagauc aagaucgccu acuacuccgu cuacaaguac     3360

aaguucagcg acaagaaccu caucaagacc gagauccgcc ugauccccau ccccaucuac     3420

cuguccaaga acgacgacga caucaaggag uacgugaaga acuaccucaa cgaccgggug     3480

aacagcaagc ugaucgacuu cacgaagauc uacgacaagc uguacgucaa cucccucauc     3540

aagaucaacg gguaccugua cuaccugggc gggaagacca acgaccgcgu guggaucgac     3600

aacgccguga gcaucguccu cgacgacaag aucgaguacc ugaucaagcg gaucuccaac     3660

gacaucaagu acggcuacga gaucaacgug agcgacgacg agcugaugga caucuacgag     3720

ucccucgccg agaaggccaa caagaagauc uucuacaacc gccugagcga cuucgagaag     3780

aucuucucca acauggacgu gaagaagaag uucgucaagc uggagaagaa cgacaagugc     3840

cgggugcucc ugcagcugcu caacgugcug accgacagcc agaccacgua caagcuggag     3900

auggucgggc ucaagcacuc ccgcagcaac aucggcauga agaucagcaa ccuggaggag     3960

uucaagguga ucaacuccag caucaccggc cuguacucca acgagaucga cauccucaac     4020

aucuga                                                                4026


<210>  1830
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1830
auggagaagg acuacuaccu gggccucgac auggggagcg gcuccgccgg gugggccgug       60

accgacgacc aguaccacgu gcugcgcgcg cacggcaagg ccaugugggg gacccggcug      120

uucgagagcg ccaacacggc cgaggagcgc cggaucaacc gcaccgcccg gcgccggcuc      180

aagcgccgga accagcgcau cgaguggcug caggagaucu ucgcggagga gaucaacacc      240

aaggacuccg gcuucuuccg gcgcaugaag gagagccggu acugggccga ggacaagcgc      300

gacgagaacg gcuccugccc cgagaugccg uacagccugu ucaucgacga ggacuucacc      360

gacaaggagu accacgagaa guuccccacg aucuaccacc uccggaagua ccugaugacc      420

accgaggaga cccccgggau ccgccuggug uaccucgccu uccaccacau gaugaagcac      480

cggggccacu uccugcugaa cgggaacauc gaggagaucc gcaaguuccg gaagacguuc      540

gaccaguucc ucgagguggu ccgcaacgag gagaugggcu ucgccgggga gaucgaggag      600

gagaccuaca agaagaucga gaugacccug aaggacguga accugacccg guccgcgaag      660

aagacgaccc ucaucaagag ccugaaggug acccgcgucg aggagaagga gcugcucgcc      720

cugaucuccg gcugcaccgu gaagcugagc aacaucuuca acgacaaguc ccucgacgag      780

acggagcgcc ccaagaucag cuucgccgac gccggcuucg acgacuacga ggccgagauc      840

caguccaacc ugaacgagaa guacuacauc aucgagagcg cgaaggccgu guacgacugg      900

gccgcccugg ccgacauccu cggggaguac uccugcauca gcgaggcgaa ggucgcccug      960

uacgagaagc accacaagga ccugaagcgg cucaaggacc uggugcgcaa caacaucucc     1020

agcgaggugu acaaggagau cuucgucgac guguccggca aguacaagaa cuacugcgcc     1080

uacaucggga ccaccaacau caacggcaag aagaaggacu ucgagggcaa gaagugcacc     1140

aaggaggacu ucuacgccua ccugaagaag uccgugaucg agugcaucaa cgacagcgag     1200

aagacggacu ggcucaagcu ggagcuggag aaggggaacu uccucccgaa gcagcugacc     1260

ggcgagaaca gcgucauccc cuaccaggug caccuguacg agcucaacgc caucaucacc     1320

aaccugcagg agcggauccc ccugcucaag cgcgacgggg agaagauccg gcagaucuuc     1380

accuuccgcg ugcccuacua cgucggcccg cugaacggga ucagcaucga cggcaagaag     1440

acgaacuggg ugaagcggaa ggagggcgug aucuaccccu ggaacuucuc cgacaucguc     1500

gacgaggaga gcuccgcgga gagcuucauc cgccggauga ccaacaagug caccuaccug     1560

auccacgagg acgugcuccc caaguacucc cugcuguaca gccgcuucga ggugcucaac     1620

gagcugaaca acguccggcu gaacggggag cuccuguccg uggagcugaa gcagaagauc     1680

uacgaggacg ucuuccagcg cuaccggaag gugacccaga agcgccugaa gcgguaccuc     1740

cugacggagg gcaucaucga cgagaacacc gugaucaccg ggcuggacgg cgacuucaag     1800

gggagccuca ccgccuacca cgacuucaag gagaagcuga cgggccugaa ccucaccgag     1860

cgcgagaagg aggagaucau ccugaacauc acccuguucg gcgacgcccg gucccuccug     1920

cgcaagcgga ucgggaagau cugcagccag cuguccgaca agcagaagga gaaccucagc     1980

aagcuguccu accagggcug ggggcgccug agcaagaagu uccucaacgg caucgccauc     2040

uccguccagg gggaggagga guggaccauc aucaaggccc ugugggagac gcaggacaac     2100

cugagccagc ucaugggcgc gaaguacacc uuccgcaacg ccguggagaa ggagaacggc     2160

gagcuggaga accgggagau cuccuacgag accgugaagg accugagcau cagccccucc     2220

gucaagcgcc agguguggca gagccuccug aucgugaagg agaucugcaa gauccagaag     2280

aagccgcccc ggaaggucuu cguggaggug gccaaggaga agaccaacuc cgggcgcacc     2340

gagagccgga agaagcgccu gcaggacuuc uacaaguacu gcuccaagua cgagaaggag     2400

cucuacgacg agagcaucca gaacaagcug gagaagugcu ccgagagcga ccugcggucc     2460

gacaagcugu uccuguacua caugcagaug ggccgcugca uguacacggg gcagcccauc     2520

gacuucgacg agcuguggaa caaccagaag uacgacaucg accacaucua cccccagagc     2580

aagaccaugg acgacuccau cgacaaccgg guccucguga agcgcaucag caaccaggag     2640

aagaccgacg uguacccgau ccgggccgac auccgcgaga agcggcgcgg cuucuggaag     2700

gagcugaaga acgucggccu gaucuccgag gagaaguaca agcggcucac ccgcgcgacg     2760

gaguucgagc ccgccgagcu ggccggguuc aucagccggc agcuggugga gaaccgccag     2820

accaccaagg uggucgccuc cauccucaag caggccaugc ccgacaccga gaucguguac     2880

gugaaggcgc ggacggucag cgaguuccgc cacaaguuca acuucaucaa ggugcgggag     2940

augaacgacu uccaccacgc caaggacgcc uaccugaacg uggucguggg caacaccuac     3000

cuggugaagu ucaccaaguc ccccgucaac uucaucgaga accacagcaa ccgcagcuac     3060

aaccucaaga agauguucac cgacgggcgc guggagcgga acggcgagau cgccuggauc     3120

uccggggaga acggcacgau cggcaccgug cgcaacauca ugaagaagaa caacauccug     3180

gucacccggc gcgcccacga ggcgaccggg ggcuucuucg accagcagcu gaugaagaag     3240

gggaagggcc aggugccggu gaaggggagc gacgagcggc uccuggacau cgagaaguac     3300

ggcggcuaca acaaggagac gggggccuac uucguccugg uggaguccga cgagaagaag     3360

ggcaagaaga agcgcaccau cgaguacgug cccauccggc ucaagaagca gcuggagaac     3420

aaccgcaagg cccuggagaa cuaccucugc gagcagcggg ggcugaccaa ccccgucgug     3480

cugaucgaca agaucaagau ggacagccuc uuccgcgugg acggcuucuu cauguggcug     3540

uccgggcgga ccggcaacca gcuccucuuc aagugcgcca accagcuggu ccugagcacg     3600

gaggacgagg ccacccucaa gaagaucgug aaguucguga accgccggaa ggagaacaag     3660

gagaucaaga ucuacgaguc cgacaagcug aacgaggaga ugcugaaccg ccucuacaac     3720

accuuccugu acaagcugca gaacagcauc uacggcaacc ggcucgggga gcaggcgaag     3780

acccugucca gcaagaagga gaacuucgac aagcugcaga ucgaggagaa gugccucguc     3840

cuguacgaga uccugcacau guuccagugc caguccgcca cgagcgaccu ccagcugauc     3900

gagggccccg ggaaggccgg cacccugcgc cucaacucca acaucaccaa guacaagaac     3960

aucaacauca ucaaccagag cccgaccggg aucuacgagc aggucaucga ccugcagaag     4020

aucuga                                                                4026


<210>  1831
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1831
augggcaagg gguacuacgu gggccuggac auggggaccg gcagcgucgg gugggccgug       60

accgacgagu ccuaccagau ccuccgccgg cacggcaagg ccaugugggg cgugcgccug      120

uucgagagcg cgaagacggc cgaggagcgg cgcauguucc ggaccgggcg ccggcgccug      180

gaccggcgcg gcuggcggau cgagauccuc caggagaucu ucgccgagga gaucucccgc      240

gucgaccccg gguucuuccu gcggaugaag gagagcaagu acuacccgga ggacaagcgc      300

gacauccagg gcaacugccc cgagcugccc uacacccucu ucguggacaa gaccuucacg      360

gacaaggacu uccacaagaa guaccccacc aucuaccacc uccggaagau gcugauggag      420

accgaggaca ccccggacau gcgccucgug uaccuggccc ugcaccacau gaugaagcac      480

cgggggcacu uccuccuguc cggcgacauc agccagguca cggaguucaa gaacaccuuc      540

aaccaguuca ucgagaacau ccgcaacgag gagauggacu ucgagaucga gcuggacgag      600

uccgccgugc agaugaucga ggagacccuc aaggacaaga accugacccg gagcgcgaag      660

aaguccaagc uggugaaggg ccucaacgcc aagaacgucc gcgacaaggc cuuccugacg      720

cugcucagcg ggggcaccgu gaagcugucc gaccuguucg ggauggagga gcucaacgag      780

ggcgagcggc ccaagaucag cuucgccgac aacggcuacg aggaguacgc ggccgugguc      840

gagauggagc ugggggagcu guacuacauc guggaguccg ccaaggccgu guacgacugg      900

gccauccuca gcgacauccu gggcgggucc accagcgucu ccgacgcgaa ggugcgcgcc      960

uacgagaagc acaaggccga ccugaaguac cucaaggccg uggucaagga guacuucccc     1020

aaggaggugu acaacgccgu guucgucgag agcuccgaca agcugaacaa cuaccccgcg     1080

uacaucggca ugaccaagaa gaacgggaag aaggugagcc uggagggcaa gcgcugcagc     1140

cgggaggagu ucauggacuu ccucaagaag aacaucgugg ucaagcugcc ggacgaggag     1200

gccaagaugu accugcaguc cgagcucgag aaggacagcu uccugcccaa gcaggucaac     1260

aaggacaacg gcgugauccc cuaccaggug cacaaguacg agcugaagaa gauccucgac     1320

aaccuggggg acaagauccc cuuccugaag gagaacgccg agaagaucga gaagcuguuc     1380

uccuuccgca ucccguacua cgugggcccc cugcggacgg ggaacggcga gaacagcaag     1440

uucgccuggg ccgagcgcaa guccaccgag aagaucuacc ccuggaacuu cgagaacguc     1500

aucgacgugg agcagagcgc ggagaacuuc auccggcgca ugaccaacaa gugcaccuac     1560

cugaucgggg aggacgugcu ccccaaggac ucccugcugu acagcaaguu caugguccuc     1620

aacgagcuga acaaccugcg gcuggacggc cagaagaucu ccguggagcu caagcagaag     1680

aucuaccgcg acguguucug ccggagccgc aaggucacgc agaagaagcu gaaguccuac     1740

cugauccggg agggcaucgc cgggaagaac guggagcuca ccggcaucga cggggacuuc     1800

aagggcagcc ugaccgccua ccacgacuuc aaggagaagc ugaucgacgu ggagcucacc     1860

cagaaggaga aggaggagau cauccugaac aucguccugu ucggggacga caagaagcuc     1920

cugaagcagc gccugaagaa gaaguucccg cagcucacgg agaagcagau caacaccgug     1980

accgcccugu ccuacaaggg cuggggccgg cugagcaaga agcuccugga ggaggugacc     2040

guccccgccc ccgagacggg ggaggugugg aacaucauca ccgcgcugug ggagaccaac     2100

gacaacuuca ugcagcuccu guccaaggag uaccaguuca gcgaccgcau cgaggaguuc     2160

aacgaggaga aggccgaccc cgaggugucc uaccagacca ucgaggaccu guacgucagc     2220

ccggccguga agcggcagau cuggcagacg cuccagauca uccaggagau cgagaaggug     2280

augggcgaga agcccaagcg cgucuucgug gagauggccc gggagaagca ggagaacaag     2340

cgcaccgagu cccggaagaa gacccugagc gaccuguaca ccaagugcaa gaaggaggag     2400

cccgagugga ucgaggcgcu cugcacgagc cuggagaagc acgaggagca ccagcugcgc     2460

uccgacaagc ucuaccugua cuacacccag aaggggcgcu gcauguacag cggcgagccc     2520

aucgaccugg aggaccucug ggacaacacc aaguacgaca ucgaccacau cuacccgcag     2580

uccaagacca uggacgacag ccugaagaac cgggugcugg ucaagaagga guacaacgcc     2640

aagaaguccg acaccuaccc caucgccgcc gacauccaga agaagaugau gcccuucugg     2700

aagucccucc ugacgggcgg guucaucccc aaggagaagu acgaccgccu ggugcggaac     2760

aacccgcucg acgccaacga gcuggcgggc uucaucgagc gccagaucgu ggagacccgg     2820

cagagcacca aggccgucgc cgagauccug aagaagaucc uccccgacac cgagaucgug     2880

uacgugaagg ccaagacggu cagcaaguuc cgccaggacu ucgacuucau caaggugcgg     2940

gacaugaacg accugcacca cgccaaggac gcguaccuga acaucguggu cgggaacgug     3000

uacuucguga aguucaccaa gaacgccgcc ugguucguca aggagaaccc cggccgcacc     3060

uacaaccucg agaagauguu caucugggac guggcccggg acggggagau cgccuggaag     3120

gcgggcaagu ccggcagcau cgugcagguc cgcgccauga uggagaagaa ccacauccug     3180

gugacccggc gcuccuacga ggugaagggc gggcuguucg accagcagau ccucaagaag     3240

gggaagggcc aggucccggu gaaggagagc gacgagcggc ugcgcgacau cgggaaguac     3300

ggcggcuaca acaaggccgc cgggguguac uucaugcugg ucuccagcaa ggacaagaag     3360

ggcaaggagc agcggacgau cgaguucgug cccaucuacc ucaaggaccg cguggagaag     3420

gacauggagu ccgcgcggaa cuaccugaug gaggagcgcg ggcugcggga gcccaagauc     3480

cucaucaaga agaucaagau cgacacccug uucaaggucg acggcuucua cauguggcug     3540

agcgggcgca ccuccaacca gcucaucuuc aagggcgcca accagcugau ccugagcaag     3600

gaggacauga agacccucaa gaagguggug aaguacaucc agcggcagaa ggaggacaag     3660

aacgucaaga ucagcaugcg cgacggcauc acggaggagg agcugcugaa gcucuacgag     3720

accuuccugg acaagauccg ccacuccauc uacggggccc ggcugagcuc ccaggagaag     3780

acccucguga acggcaagga gaaguucauc gaccugagcg ucgaggccaa gugccaggug     3840

cuguacgaga uccuccaccu guuccagugc caguccgggg ccgcgaaccu gaagaugauc     3900

ggcgggcccg ccagcgccgg cauccucgug augaacaaca acaucuccaa gugcaagaac     3960

aucagcaucg ucaaccaguc cccgaccggc aucuacgaga aggagaucga ccugcugcgc     4020

guguga                                                                4026


<210>  1832
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1832
augacccaga aggugauccc cuacaacauc ggccuggaca ucgggaccaa cagcgucggc       60

ugggccguga cggacaacga gaacaagcuc cugcacauca aggggcacaa cggcaucggg      120

gugcgccugu ucaaggaggg ccaguccgcc gcggagcggu gcggcuuccg caccacccgg      180

cgccggcuca gccgccggaa guggcgccug cggcugcuca acgagaucuu cgccgacaag      240

aucgccaagg ucgacccguc cuucuucgcc cgccugaagc agagcaacgu gucccccaag      300

gaccccaaca agaccauguu cgggaacauc cuguucgaca acggcaaccu caacgacaag      360

agcuuccacg acgaguacaa gacgaucuac caccuccggc aguccaucau cgaccacccc      420

gagaagaagu ucgacauccg ccugaucuac cuggccaugc accacaucau caaguaccgg      480

gggcacuucc ugaaccaggc gaacgugcag gacuucaagg gcgggaagau cgaccuggag      540

gagagcuuca aggaccucaa cuccaucuuc gagaaccagg gccgcgaccu gcggcugguc      600

accgaggacg ccaacaagua cgugaaggag cucagcgaca acuccaagac ccgcaacgac      660

cggcagaagg agcugagcaa ggugcuguac aucccgaucg acaagaacuc cgacaagaac      720

aacaagaagg ucaccacgga gaucaucaag gccauccucg gcaugaaggc caaguucgac      780

gugaucuucg ggaucgacgc ggagaacgcc aaggagugga gccugaccuu caacuccgac      840

gacuucgacg acaagaucga gggccugagc gcccagauga ccgacgaggc cggggagauc      900

cuccugaucc ugaaggagcu cuacuucgcc cugaaccugu ccgacauccu caacaacagc      960

gugaccgaca agaacgcgaa gacgcugucc gaggccauga ucaaccgcua cgacgaccac     1020

aaggagcagc ugaccaugcu caagcagguc aucgacaaca gcgacaagaa gcacgccaag     1080

gcccugaagg ccgcguacag cgccuacauc gacggcgaga acaacaagaa gaucuccuuc     1140

gaggaccugc ggaagcgcau ccagaagaac cucgacaaca gcgagaccuc caagaagauc     1200

aacgaccugc uggagcacga cuacuuccuc cccaagcagc ggagcaagga gaacggcgcc     1260

aucccccacc agcugcagca gcaggagcug gacaagauca ucgaguccca gaagcacuac     1320

uaccccuucc ucgccgagcu gaacccgaac gagaagcgca gccgccaggc caaguacaag     1380

cuggacgagc ucguggcguu ccgggugccc uacuacgucg ggcccauggu gaaggccacc     1440

gagaacaacc aguccaacaa cggcaaguuc gccuggauga ucaagaacga caacaucagc     1500

gacaagcuga agaaggagga gaucacgccc uggaacuucg acaagaaggu ggaccgcacc     1560

gagaccgcca ccaaguucau cacgcggaug accaccaccg acacguaccu gaucggggag     1620

ccgguccucc ccaagaacuc ccugcuguac cagaaguucg uggugcugaa cgagcucaac     1680

aaccugaaga ucaacaagca gcccaucacc cccgagcaga agcaguacgu cuacgagcac     1740

gugaugaagc agaccaagcg cgugagccug aagaagaucg ccgacgcgcu cgucaucaag     1800

ggcgacuacc cguacgcccc cgagaucacc gggacgaccg accagaagaa caugaacaac     1860

ggccugucca ccuacaucga ccuggccaag aucuucggcg acgagcucga cgacaucgac     1920

cggaaggagg acuucgagaa gaucaucgag uggagcacca ucuucgagga ccagcacauc     1980

uucgcccuga agcugaagga gaucaagugg cucacggagg accagcgcaa gcaggucgug     2040

aagcugcggu accaggggug gggcaagcug uccaagaagc uccuggcgga ccugguggac     2100

gacaacgggc agcgcaucau cgaccuccug ugggacagca agcaggacuu caugcagauc     2160

gugaaccaga acuccuucaa gcaggccauc gccgagcaca acggcgccca ccuggaggag     2220

aaggaccuca aggacaucau caacaaccug uacaccagcc cccagaacaa gaaggccauc     2280

cggcagguca uccugguggu ggacgacauc auccgcgcgg ucggguacga gcccgugaac     2340

aucaugaugg aguucgcccg ggaggacucc aaggaccacc gccucaccaa cagccggucc     2400

cgccagcugg agaaggugua caaggacauc accaacagcu gguucgacga cgacaacguc     2460

aaggacgagc ugaaggacaa gaucaaggac aaggccaagu ucacggaccg gcucuaccug     2520

uacuucaccc agggcggcaa ggaccuguac accggggagc cgcucgacau cgacaaccug     2580

agcaacuacc agaucgacca cauccugccc caguccuuca uccucgacaa cagccuggac     2640

aaccgcgugc ugaccuccaa gaucaacaac cagaucaaga aggacaaccu ccccggcgag     2700

uucuucggga gcaagaugca caacuucugg aagcaccuga aggagcugca ccucaucucc     2760

aacaagaagu acuacaaccu gacccugacg cccaacagca ucuccaaguu caacaaccag     2820

ggccgguuca ucaaccgcca gcucguggag acccggcagg ucaucaagcu ggccgccgag     2880

auccugcaca accgcuacag cgaggagaac gggaccaaca ucgugaccgu gaaggcgaac     2940

cucacgcacc acaugcgcga gaaguucaac uucuacaaga accggaacgu caacgacuac     3000

caccacgccu ucgacgccua ccuggccgcc uucgugggca acuggcugcu ccagcaguac     3060

ccgaagcuga agcccuacuu cguguacggc gacuucgcga agaccgagau cgacaaccug     3120

aaguccuuca accucaucua caaguucgag caggcccacc agcugaacga ggaggacggg     3180

aagaucgcca aggacaccga cgcccugcuc ggcuacauga agaacgucua ccacuucaag     3240

aagaugcugg ugaccaagga gcuggagacg aaccacggga accucuucaa gcagacccug     3300

uaccccagcc acggcaagau gaagaaccug aucaacccca aggagaaccg cuccagcgac     3360

aucuacggcg gguacuccag cgagaccggc gcguacgugu cccucaucaa ggucuucaag     3420

aagaacaaga ucgagaacaa gaucaucaag aucccgcugc uguacaucga caagcucaac     3480

cggcaggcca acgccaagca guucauccgc aacuucauca ucaaggacga cagcaacaag     3540

uucgacaucc uguccgagaa gaucuuccug aaccagcucg ugaucgacgg ggacgagcug     3600

uucaccauca gcggcagcgg ggaccggcag aacgcccagc agcugauccu cuccgacaag     3660

agccugaaga uccuguccaa gaacaucaac gugaucagca agaacgaccu caucaucauc     3720

uucaacgaga ucaucgacga caucugcaag uacaugaaga uguacgaggu cgagaacucc     3780

gacgagacga aggccaaguu cgaggagauc aacgacaagg accgcaugcu gaagaucauc     3840

aacgaccugc uccugggccu gcacgcgaac gccagccggu ccaaccucag cuccaucggc     3900

cugagccccu ccuucgggcg cuucuucagc ggcaacgccg ggaucaagcu guccgagaac     3960

gccaaguuca ucuaccagag ccccaccggc cucuucuccc ggaaggugaa gaucagcgac     4020

cuguga                                                                4026


<210>  1833
<211>  4032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1833
augaccuacu accugggccu cgacaugggg accagcuccg ugggcugggc cgugacggac       60

gagaacuaca accugaucca ccgccaccgg aaggaccugu gggggauccg cgaguucgag      120

gaggccaaga ccgcggugga gcggcgcacc cagcggauca gccgccggcg ccggcagcgc      180

gagguggccc ggaucggccu ccugaaggac uacuuccacg acgagaucca gaaggucgac      240

cccaacuucu accagcgccu ggacaacucc aaguacuucc cggaggacaa ggaggaggug      300

gugaagggga agaacggcau cuucgccgac gagaacuaca ccgaccggga cuacuucgcc      360

gaguacccca cgaucuucca ccuccgccgg gagcugcuga acagcaagga gccccacgac      420

guccgccucc uguaccuggc ccuccugaac auguacaagc accggggcca cuuccugaac      480

accacccucg ggaccgacgg cgagaaccgc aagcuguccg acguguaccg ggacuucgug      540

gucaagacgg aggaggugcu ggggcucacc uucccccagg agaccgacaa cggcaagaug      600

caggagauca ugugcagccg cgaguucucc cggaagcgca aggcggagcu gcugugcgcc      660

cuccuggaga ucgacaagaa gcagaagcag caggucgccc ugaucgagug cgugcucggg      720

cuggacaaga gcgugaagca gauguacggc accgccgugc cggacgacuu cgacaagucc      780

gucaagguga acuucagcga cuccagcuuc gaggagaagg uguccgaggu cgaggagcug      840

cucgacgagg agaaguacga gcugauccug ugcaugaagg agcucuacga cacggcggug      900

cugaaccaga ucaugggcga cugcaacuac cugagcgagg cccgcgugca ggaguacgag      960

aagcacaagg aggaccucaa gcugcugaag cggaccguca agaaguacgg gaccgccgag     1020

gaguaccagu uccucuuccg cuccagcgag aaggccuccu acaacgccua cgugcacagc     1080

gugaacuccg gcgagaagau ccggcgcaac auggacgggc ggagccgcga cgaccuguac     1140

accguccuga agaagaugcu caagaacuac ccccaggagg acgcggacug caaguacauc     1200

cuggaggaga ucgagaagga gcaguuccug cccaagcagc ucgugagcga caacggcguc     1260

auccccaacc agauccacua caaggagaug aagaagaucc uggagaacgc cgagcaguac     1320

cugcacuucc uccgggagaa ggacgagucc ggcuacacgg uggccgagcg cauccugaag     1380

cuguucgccu uccagauccc guacuacauc gggcccacca gcgagaaguc cggcaagaac     1440

gggggcaacg gguggguggu ccggaaggag cagggccagg ugcuccccug gaacaucgag     1500

gagaagaucg acaugaaggc caccagcgag accuucaucu cccgcauggu gcggcgcugc     1560

acguaccuga gcgacgagau gguccugccc aaggcguccc uggaguacga gagcuucugc     1620

gugcucaacg agcugaacaa ccugcggauc cgcggcguga agcucccgau caccgucaag     1680

caggacaucu acaaggaccu guucgagcgg gggaagcgcg ugacccggaa gcagaucgug     1740

aacuaccuga agggcgucgg gauccuccag gagggcgagg aggacgagcu gaccgggaug     1800

auggacacgu ucaacuccag ccuggccucc uacggcaagu ucaagagcau ccucggcgag     1860

aagauccagg aggacuccug caagcgcaug guggagcaga ucaucuucug gugcaccauc     1920

uacggggacg ccaagaaguu ccugaaggag cagcuggagg agaaguacgg cgacgugcuc     1980

acccgggagc agaucaagcg cauccugggg uucaagcuga aggacugggg caaccucagc     2040

aagaaguucc uggagcugaa cgggugcgac cgcaacaccg gcgaggucau gucccucauc     2100

cggaugcugu gggagagcga cgacaaccug auggagcucc ugaaggacuc cagcuacacg     2160

uaccgcgccg agcuggagga gcagcaggag aagcucacca agacccugac cgagaucugc     2220

cccgaggacc uggagggcua cuacuucucc gcccccguga agcggaugau cuggcagacg     2280

cuccugaucc ugaaggaggu ggagaagguc augggggagc ccccgaagcg cguguucgug     2340

gagaugaccc gggagcacga cgagcgccgg gagaugaagg acagccgcgg caagaaguuc     2400

caggagcucu acaagaacau caaggacgag gagcacaacu ggaaggcgga gaucgaggac     2460

gccgagaagg acgggcggau ccgcagcaag aagauguacc uguaccugac ccagcggggc     2520

cgcugcaugu acaccggcaa ccccaucgac cucgaggagc uguucacgac caaguacgac     2580

aucgaccaca ucuacccccg gcacuucguc aaggacgaca accuggacaa caaccucgug     2640

cugguggaga aggagaagaa cgcccacaag uccgaccagu acccccuggg ggacgacauc     2700

uacaccggcc gccucagcug guggaaggag cugcaccggg agaagcugau caacgacgag     2760

aaguacaagc gccucaccgg gcgggccccg uucuccgacg agcagaaggc cggcuucauc     2820

gcgcgccagc uggucgagac cagccagggg acgaagggcg uggccuccau ccugaaggag     2880

gugcuccccg agaccaccac caucgucuac gccaaggccc ggaacgugag cgacuuccgc     2940

aacacgaacg agcugcugaa ggcgcggucc gugaacgacu uccaccacgc ccaggacgcc     3000

uaccucaaca ucgucguggg caacguguac uacgucaagu ucaccaacaa cccccugaac     3060

uucgugcgca gcggggaggg cuacaaccug gacggggugu uccgcaagaa cgucauccgg     3120

ggccaggaga acgccuggau cgugaagccc gagaaggggg agaagaugac ccucgagacc     3180

gugaagcgcg ucauggccaa gaacacgccg cugcugaccc ggcucuccuu cgagggccgc     3240

ggcgggcugg cgaaccagac ccuguacagc gccaaggagg ccgccggcga gggguacgug     3300

ccccucaagu ccagcgacuc ccggcugcag gacgugacca aguacggcgg guucacgagc     3360

gucaccaccg ccuacuucau ccugguggag cacgacauga agaagaagcg cgugcggacc     3420

aucgaguccg uccccauccu cuggaaggag cgcaucgaga aggagagcgg ccagcuggag     3480

aucuacugcc gggaggcgcu gggccucgug aaccccgaca uccgcgugcg gaagauccug     3540

auccaguccc uggucaagaa ggacggguuc uacaugcacc ucagcggcaa gacggggaac     3600

cgcuucauca ugcggaacgc cguggagcug ugccugaagc aggaguggau caacuacagc     3660

aagaagcucg agaaggaggg cugcgacgag uccaucacca aggagaagaa cgaggagcug     3720

uacgagaucc ugaaggagaa gcacaucaac gggaucuacc gcaagcggcc gaaccccaug     3780

ggcgagaagc uccacauccg ccaggagacc uuccggaugc ugacccugga ggagcagaag     3840

aaggugcucc ugagccugcu caaccugacg aagaucggcc ccgucgaggc caaccuggag     3900

cucaucgggg aguccgccca caccggcaag augcugauca gcaagaacau caccgaggug     3960

aaggaguucu accugaucca ccaguccguc accgggaucu acgagaagca ggucaaccuc     4020

cugacggugu ga                                                         4032


<210>  1834
<211>  4032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1834
auggccacca agaaggagua caacaucggc cuggacaucg ggaccacgag cgugggcugg       60

uccgucgugg agaccgacaa ccagaaggug augcgcaagg ggaacaaggc ccucuggggc      120

guccggcugu ucgaggaggc gaccaccgcc gagagccgcc ggaugcagcg cuccacgcgg      180

cgccgguacg accgccggcg cgagcggauc aagcugcucc aggaggaguu cagcgaggag      240

aucaacaagg uggacaagaa cuucuuccag aagcugaagg aguccaagua cguggagaac      300

gacaagauca acaagaagau cguccugacc aaggaggaga agcaggagcu caaggacuac      360

cagaacaagu acaagaccau cuaccaccuc cgcgacgagc ugaucaacaa ccccgagaag      420

aaggacaucc ggcuggugua ccuggccauc caccacauca ucaaguaccg cgggaacuuc      480

cuguaccaga acgccaacuu caacaucgac aaccucgaca ucaaggacaa gcugaacgag      540

cuguucagca uccucuccaa caacauccag gagcuggaga ucccggagga cuacaucaac      600

aucaucgacu ucgacgagcu ggaggccgac cuccugaagg acaccaagaa cgacgugaag      660

gcgcugcuca ucgacaaccu gacggagcug accaacaaga gcuccgccac cgaguucggc      720

aagcucaugg ucggcaacaa ggggaacauc aacaagcugc ugaugcucga gaccgacaac      780

aagaucgaga ucagcuucuc cggcacggac uacgacgaca aguacgagga guaccaggag      840

gcccuggggg agaacaucga gauccuggac auccucaagc agaucuacga cugcaucuuc      900

cugaagaaga ucuucaaggg caacaagaac accagcaucu ccagccugau ggugaagucc      960

uucgagcagc acaagaagga ccucaacuuc cugaaggagc uguucaagga caaccgggag     1020

cucuacaacg agaucuuccg caccaagaag gaccugugcc uguacgagaa guacaucacc     1080

aacaagaucg acugcgacga guuccggaag gagaucaaca agcuccugga gagccuguuc     1140

uccgacgaga acaucaagca gaaccuccug gacgaguaca accuggaggu gaaggagcgc     1200

aucgagaacg ggacguuccu cccccggauc accaccaccg acaacggcaa guaccccuac     1260

cagcugaaca agagcgagcu gaucaagauc aucgagaacc agggcaagua cuaccccuuc     1320

cuccugaaca agcugaacga caacaagacg uacaagaucg ucaagcugcu cgaguuccgc     1380

aucccguacu acguggggcc ccuggugucc gagaagcgga gcgagaacag cuggcuggag     1440

cgcaagauca acaacgucaa gaucaccccc uacaacuucg acgacguggu ggacaaggag     1500

aagaccgccg agaaguucau caagcgcaug aucucccacu gcaccuaccu ccuggacgag     1560

uacgcgcugg ccaacaacag cauccucuac uccgaguuca aggucaugaa cgagcugaag     1620

cagauccggg ugaacgaccg caagcuggag cucaaguucc agcagcagau ccuggaggac     1680

uucuucaaga agacgagcgg caccaucacc gagcggaagu ucaagaacua ccugaucucc     1740

accggggagu acagcaugga gggcggcaac uuccgcauca cgggguacuc cgccgacggc     1800

aaguucgcca acaacaugca gagcuacgug gacuucuucg gggagaacgg caucuucgcc     1860

gacaccgacu acaaccucga gaacgcggag gagaucaucg aguggaucac caucuucgag     1920

gacaaggaca uccuggagaa gaagguccgg gacaacuacu ccaagcugaa cgagaaccag     1980

aucaagaagg ugcucgccaa gaaguacagc ggguggggcu cccugagcaa gaagcugcuc     2040

aagaccaagu acuacaagga caaggccacg gagcuguaca aguccauccu ggaccucaug     2100

uacgagaccg acaagaacuu caugcagauc aucaacgacg acaaguacaa cuuccaggag     2160

augaucaaga aguucaacaa caaggaggag aacaagaagc ugaacuacag caucguggag     2220

gagcuggcca ccucccccgc caccaagcgc ggcaucuacc aggcgcucaa ggucguggag     2280

gagcuggucg acuacaucgg guacgagccg aagaacauca gcaucgagau ggcccggucc     2340

gaggaggaga aggugcgcaa ggacagccgg aaggacuaca ucaacaaccu guacaagggc     2400

ugcaaggacu ccaucgagaa cuacaagaag cucaagcacg agcuggacag ccacgagauc     2460

acgagccagc gccuguuccu cuacuucauc caggagggga agugccugua caccggcacg     2520

ccccugaaca ucgaggacau cgagaaccag ucccucuacg agaucgacca caucaucccc     2580

cggagccuga ucaaggacga cuccaucgac aacaaggccc uggugcucaa ggagugcaac     2640

caggacaaga aggccagcua cguccugccc uccaaguucc gcaacagcaa gcagaagacc     2700

ugguggaagc accugaagga caacgggcuc auguccgcca agaaguucca caaccugauc     2760

cgggagaagu acaacgagga ggacaucaac ggcuucauca accgccagcu gguggagacc     2820

cggcagauca ccaagcacgu ggcgaacauc cucaacaacc uguacaagga caccaacguc     2880

aucuaccuga aggccgaccu cagccacaac uaccgcgaga aguacgaccu guucaaguuc     2940

cgggacauca acgacuacca ccacgcccac gacgccuacc uggccgcggu gcucggcgag     3000

uacaaggagc gcuucaugaa gcggaagauc aacuucgaca uggugaagga gcugaacucc     3060

aagaucgucg agauggacga ggggaagcgc cgcaaccugc gguucggcua cgugaucaac     3120

agccucgacg agaaccugaa cgacaucgug cugaagaucu ccgagaaccu cgucgacaac     3180

gagaccgggg aggugcuguu cgacgcccac gaguucaaca agcgcgugga ggacacccug     3240

uaccggaacg acauccucau cagccgcaag acggagaucc ggaccggcca guucuacaag     3300

gagaccaucu acaagaaggg gaagggcacc aucccgauca aguucggcau gcccacggac     3360

aucuacgggg gcuacuccaa caccaacgcc cgcaagcuga ugcugaucga guacaacgag     3420

agcaagauga agaucaucgg gcuccccaug gcccuggcgg uccggaacaa cgacgccgag     3480

aucaacgacu acaucaagac ccagaucaac cugaagaaga acuccaccuu caagaucaag     3540

aagugcagca uccccuucga gacggagauc aucuacaaca accagaacgu guucaucaag     3600

ggcuacucca ucggccacaa gaacugcgag aucagcaacg ccauccagcu caagaucgcc     3660

aaggacaaga ugaagcacug gaaguacgcc cugcaguacc ugcucaacga caagaagcag     3720

uacaucgacu ucgcggagaa guacgugaac gagaucuacg acuuccugau caaccuggac     3780

cucuucccgc uguucaacaa ggagcuggac aagaucaaga acaaggucaa cuucgaggac     3840

cucgcccugg accagcagaa gaagaucacc aaggagcugu ucaagaugcu ccacugcaac     3900

agcgugaacg ccaaccuguc cgaguucggg cugggcgacc gcauggggcg gcucagcggc     3960

uacaacauca ccgacgggau caucaucucc aagagcguga ccggcauccg cgagucccgg     4020

uacgaguucu ga                                                         4032


<210>  1835
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1835
augagccaga acaacaacaa gaucuacaac aucggccugg acaucgggga cgccuccgug       60

ggcugggccg ucguggacga gcacuacaac cuccugaagc gccacgggaa gcacaugugg      120

ggcagccggc uguucaccca ggcgaacacc gccguggagc gccgguccag ccgcuccacg      180

cggcgccggu acaacaagcg ccgggagcgc auccggcucc ugcgcgagau cauggaggac      240

augguccugg acguggaccc caccuucuuc auccggcucg ccaacgugag cuuccuggac      300

caggaggaca agaaggacua ccugaaggag aacuaccacu ccaacuacaa ccucuucauc      360

gacaaggacu ucaacgacaa gaccuacuac gacaaguacc cgaccaucua ccaccuccgc      420

aagcaccugu gcgagagcaa ggagaaggag gacccccggc ucaucuaccu ggcccugcac      480

cacaucguca aguaccgcgg gaacuuccuc uacgagggcc agaaguucuc cauggacgug      540

agcaacaucg aggacaagau gaucgacgug cugcggcagu ucaacgagau caaccuguuc      600

gaguacgucg aggaccgcaa gaagaucgac gaggugcuca acgugcugaa ggagccccug      660

uccaagaagc acaaggccga gaaggcguuc gcccucuucg acacgaccaa ggacaacaag      720

gccgccuaca aggagcugug cgcggcccug gccggcaaca aguucaacgu caccaagaug      780

cucaaggagg ccgagcugca cgacgaggac gagaaggaca ucagcuucaa guucuccgac      840

gccaccuucg acgacgcguu cguggagaag cagccccugc ucggggacug cguggaguuc      900

aucgaccugc ugcacgacau cuacagcugg gucgagcucc agaacauccu gggcuccgcc      960

cacacgagcg agccguccau cagcgccgcc augauccagc gguacgagga ccacaagaac     1020

gaccugaagc uccugaagga cgugauccgc aaguaccugc ccaagaagua cuucgaggug     1080

uuccgggacg agaaguccaa gaagaacaac uacugcaacu acaucaacca ccccagcaag     1140

acccccgucg acgaguucua caaguacauc aagaagcuca ucgagaagau cgacgacccg     1200

gacgugaaga ccauccugaa caagaucgag cuggagagcu ucaugcucaa gcagaacucc     1260

cgcaccaacg gggccgugcc cuaccagaug cagcuggacg agcugaacaa gauccucgag     1320

aaccagagcg ucuacuacuc cgaccugaag gacaacgagg acaagauccg cagcauccug     1380

acguuccgga uccccuacua cuucggcccc cucaacauca ccaaggaccg ccaguucgac     1440

uggaucauca agaaggaggg gaaggagaac gagcggaucc ugccguggaa cgcgaacgag     1500

aucguggacg uggacaagac cgccgacgag uucaucaagc gcaugcggaa cuucugcacc     1560

uacuuccccg acgagcccgu cauggccaag aacucccuga cggugagcaa guacgagguc     1620

cucaacgaga ucaacaagcu gcgcaucaac gaccaccuga ucaagcggga caugaaggac     1680

aagaugcugc acacccucuu cauggaccac aaguccauca gcgccaacgc caugaagaag     1740

uggcugguga agaaccagua cuucuccaac accgacgaca ucaagaucga gggcuuccag     1800

aaggagaacg cgugcagcac cucccugacg cccuggaucg acuucaccaa gaucuucggc     1860

aagaucaacg agagcaacua cgacuucauc gagaagauca ucuacgacgu gaccgucuuc     1920

gaggacaaga agauccuccg ccggcgccug aagaaggagu acgaccugga cgaggagaag     1980

aucaagaaga uccucaagcu gaaguacucc ggguggagcc ggcuguccaa gaagcuccug     2040

agcggcauca agaccaagua caaggacucc acgcgcaccc cggagaccgu gcuggaggug     2100

auggagcgga ccaacaugaa ccucaugcag gucaucaacg acgagaagcu gggguucaag     2160

aagacgaucg acgacgccaa cagcaccucc gugagcggca aguucagcua cgccgaggug     2220

caggagcugg ccggcucccc cgccaucaag cgcgggaucu ggcaggcgcu ccugaucguc     2280

gacgagauca agaagaucau gaagcacgag cccgcccacg uguacaucga guucgcccgg     2340

aacgaggacg agaaggagcg caaggacagc uucgugaacc agaugcugaa gcucuacaag     2400

gacuacgacu ucgaggacga gaccgagaag gaggccaaca agcaccugaa gggcgaggac     2460

gcgaagucca agauccggag cgagcgccug aagcucuacu acacccagau ggggaagugc     2520

auguacacgg gcaagucccu ggacaucgac cggcuggaca ccuaccaggu cgaccacauc     2580

gugccccaga gccuccugaa ggacgacucc aucgacaaca aggugcuggu ccucagcucc     2640

gagaaccagc gcaagcugga cgaccuggug aucccgagcu ccauccgcaa caagauguac     2700

ggguucuggg agaagcuguu caacaacaag aucaucagcc ccaagaaguu cuacucccug     2760

aucaagaccg aguucaacga gaaggaccag gagcgguuca ucaaccgcca gaucguggag     2820

acccggcaga ucacgaagca cgucgcccag aucaucgaca accacuacga gaacaccaag     2880

guggugaccg uccgcgccga ccugagccac caguuccggg agcgcuacca caucuacaag     2940

aaccgggaca ucaacgacuu ccaccacgcc cacgacgccu acaucgcgac cauccucggc     3000

accuacaucg gccaccgcuu cgagucccug gacgccaagu acaucuacgg ggaguacaag     3060

cggaucuucc gcaaccagaa gaacaagggc aaggagauga agaagaacaa cgacggguuc     3120

auccugaaca gcaugcggaa caucuacgcc gacaaggaca cgggcgagau cgugugggac     3180

cccaacuaca ucgaccgcau caagaagugc uucuacuaca aggacugcuu cgugaccaag     3240

aagcucgagg agaacaacgg gaccuucuuc aacgucaccg ugcugcccaa cgacacgaac     3300

uccgacaagg acaacacccu ggccaccgug ccggucaaca aguaccggag caacgugaac     3360

aaguacggcg gcuucagcgg ggugaacucc uucaucgucg ccaucaaggg caagaagaag     3420

aaggggaaga aggugaucga ggugaacaag cucaccggca ucccccugau guacaagaac     3480

gcggacgagg agaucaagau caacuaccug aagcaggccg aggaccucga ggagguccag     3540

aucgggaagg agauccugaa gaaccagcug aucgagaagg acggcggccu cuacuacauc     3600

guggccccca cggagaucau caacgccaag cagcugaucc ugaacgagag ccagaccaag     3660

cucgugugcg agaucuacaa ggccaugaag uacaagaacu acgacaaccu ggacuccgag     3720

aagaucaucg accuguaccg ccuccugauc aacaagaugg agcuguacua ccccgaguac     3780

cggaagcagc ucgucaagaa guucgaggac cgcuacgagc agcugaaggu gaucagcauc     3840

gaggagaagu gcaacaucau caagcagauc cuggcgaccc uccacugcaa cuccagcauc     3900

gggaagauca uguacuccga cuucaagauc agcaccacga ucggccggcu gaacgggcgc     3960

accaucuccc uggacgacau cagcuucauc gccgaguccc cgaccggcau guacagcaag     4020

aaguacaagc ucuga                                                      4035


<210>  1836
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1836
augaagaaga ccaaccccua cauccugagc cucgacaucg gcaccgccuc caucggguac       60

gccugcaugg accuggacuu caacauccug aaguaccacg acaaggacgc gaucggcgug      120

cucacguucg agggggccga caccgccgcc gagcgccggg gcuuccgcac cagccggcgc      180

cggaagaacc gccggaucaa gcgccugggg cugcuccagg agguccuggc cccgcuggug      240

aagaacccca acuacuacca gcuccugaac cugcacaagu ggaagaacuc caacgaggac      300

uucaagcacc ggagccucuc cgaggugcug aagauccugg gcaagaaccc caagaccuac      360

cccacgaucu accaccucca gcaggacauc cagaaccacc cggagaagaa guucgagccc      420

gagcugaucu uccuggcgcu cuaccaccug gucaaguacc gcggccacuu ccuguucaac      480

aaccucagcu ucgacaaggg gaagaccgug gacaacgagg cccagcuguc cgagcugcuc      540

gagcaguacc agagcaucaa ccccuacgag gacuccauca gcaacaagga caucaacgug      600

aucgucgaca cccugaagga cgaccagcug acccggaacg acaaguccaa ggagcucaag      660

cgcaacguga gcaagacgca cgccgagcug uucaagaugc uggugggccu caaguucaag      720

caguccaucc uguucgagaa cauggagaac gccgaggaca ucaaggagag cgcgaccggg      780

uccgacgcca ucagccuguc cgacgacuac uacgagaacc ucagcgaguu ccugaccgac      840

gagcagcuga acuucaucga guccgccaac accuucuuca ugggcaucau gcucgaccgg      900

auccugaagg gggccacgag caucagcgcc gcgaaggucc gcgacuacaa gaucuucaag      960

aaccagcuga agaucaucaa ggacaucgug uaccagaaca aguacgacaa guaccacgag     1020

uucuucguga ccuccaagga ggacaugcgg gccuaccagg agaaccccga cgccaagacc     1080

uucggcaagc ucagcaaguu cgaccaguac cugauccacc cgaaggacca guacgccaag     1140

cugaugaagg accucaaggg caucgucccc aagaaguaca ccgaggugcu gaaccugauc     1200

gacgagcaga aguuccucgu gguccagaac acgaccgaca acgccuccau ccccuaccag     1260

aacaacgugu ucgaggcgga gaacauccug cgcaaccagc agaaguacua ccccgagauc     1320

accgaggaga ugaucgggaa ggugaucaac cugaucagcu uccggauccc guacuacguc     1380

ggcccccuca ccaacaagaa cgggcagucc gacuucgccu ggaugauccg caagagcgac     1440

gagcagacgc ugcccuggaa cuucgacaag gugaucgaca aguccaagag cgccgagaag     1500

uucauccggc gcaugaccuc caagugcacc uaccugcugg aggaggacgu gcuccccaag     1560

cacagccugc uguaccagga gauggagguc cucaacgagc ugaacaacgu gcagcugcgg     1620

ggcgagaacg agccgaagca ccgcauccac cgccucgacc ccaaggugaa gcaguucauc     1680

uaccacaacc uguuccagaa gaagaagaac gucacccaca aggaccugau cacgcuccug     1740

cagaacuccg agcaccggaa cacccugaac aagaccagcg gccagaagcu caagguguuc     1800

gggacccaga acgacuccaa guucgccagc aagcugucca cguacaacga caugaaggcc     1860

aucuucggca gcgucgagaa caagcgccag augaucgagg agcuggugcu cuggaucacc     1920

aucuucgagg acaaggcgau ccugaagcag aagaucgggg agaaguaccc caacaucacc     1980

gagaagcagc ugaaccagau cgugaagcuc aacuacuccg gcugggggcg gcugagcgag     2040

aagcugcuca ccaaggaggu caacggccgc ucccugaucg accugaugua cagcacggag     2100

aacaacuuca uggagaucau caccgacaag acccucggcu ucgacugcuu caucaagaac     2160

agcaacaagg ugaagaacgc caagaucucc uacaaggacg uggccgagcu gcggaccagc     2220

cccgcccuga agaaggggau cuggaacgcc aucaagaugg uccgcgagau cuccgaccuc     2280

uucggcgcgc cggagaagau caucaucgag uucgccacgg aggaccaggu gaaggggaag     2340

cggcagaagu cccgcagcga gcugugggac gaccugguga agaagaacaa ccucaagcgg     2400

aacaagaagu ucaagggccu guucgaggag cugaaggccu accccgaccu cgacuucagc     2460

aaccccaagc uguggcugua cauccaccag aacgggaagu gcauguacac caagaagccc     2520

aucgaccucg agcgccugau guccgacacc aacaaccagc uguacgagau cgaccacauc     2580

cucccgcgga ccuucgucaa ggacgacagc aucaacaaca aggugcuggu gauccagucc     2640

gccaaccaga agaaguccgg cgacaagaug ccccuggaga ucuucgacaa ccacguccuc     2700

gugcccuucu ggaagaugcu guacgagaac gaccugauca gcagcuccaa gcucagcaag     2760

cugaugaaga aggaguucaa cgaccuggac aaggagggcu ucauccagcg ccagcucgug     2820

gagacccggc agaucaucuc ccacgucagc gacuuccuga cggaggagua ccccuccacc     2880

accguggugc cgaugaaggc gaacgucgug agcgagcugc gccggaaguu cgacaucccc     2940

aagauccgcc agcucaacga cuggcaccac gccguggacg ccuaccugaa cggguucguc     3000

uaccaggccg cccggguguu cuaccccaag gugaaccugu ucgaguucaa cuucaagugg     3060

gagaaggucc gcgagaagug gcggcagcuc ggcgaguuca ccgagaacaa caagaagcgc     3120

gaguucuucc uguucaagcg cauggagaac uacgugaacg agcacgggga gggcaugauc     3180

agcaagcuga ucuucgaccu cgacaacuuc aaguucaacu acucccggaa gguggagagc     3240

acggaguccg cguucuacaa gcagagcgcc uucuccccga agaacaccac cgcccaguac     3300

gacagcgaca agaacaagac cgccaucuac aaggagauga agaacaagcg cgggaucguc     3360

guguccuaca cguacgugaa gaaggaccag gagcugaucg gcuacggcgu cuucaacgag     3420

accguguucg aguacgugaa caacgccgac gucgacauga acacccgggc gcugaagauc     3480

gugcgcaagg ccuuccccaa gcucgacauc aagaaggccc aguaccuguu cagcaucaac     3540

aagggggacg cccugcucgu gaacggccac cccuuccugu ucgucuccgc caaggagcgg     3600

aucaacggga agcagcugga guucgacauc aacaugcagc gccggcucaa gaaggcgcug     3660

cgccccuaca acaagaugac cgagcaggag aaggugggcg uguacaacca guucgccgag     3720

aaggucgugc aggaguacga gaacagccug ggcgccaaga cgcggaacga gaagcucgag     3780

cugauccgca accaguucaa cgaguccgac aagagcaacg aggcccugau ccagugcauc     3840

cucgaggugc ugaagaugac caaggucucc gcggagcgga gcgugaagcu gaagauccgc     3900

uggggguccc agaccccgaa cacccucauc gacaucaaca acaaggccaa gaucauguac     3960

aacagcauca cgggccugaa gcagacccgg cugaagucca ucuacaagcu cgccgagagc     4020

aagaacgagc uguga                                                      4035


<210>  1837
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1837
augaacaaga acuacuacau cggccuggac auggggacca acagcguggg cugggccguc       60

accgaccagg aguacaaccu caucaagcug aaggggacgc acgccugggg cgcgcgcauc      120

uuccaggagg ccaaggacaa gaaggggacc cgggccaucc gcuccagccg gcgccggaug      180

gaccgccgga aguaccgcau ccaccuccuc aaccagcugu ucgcccagga gauccaggcc      240

aucgacucca ccuucuucau ccggcuggcg uacagcgccu acugggaggu ggacaagaag      300

gacaagggca ucggccgcaa cauccucuuc aagaccaagc aggaggaguc ccaguucuac      360

aagaaguacc ccacgaucug gcaccuccgc aagagccugc uggagaacca gccggacgcc      420

uucuccgacc ugcgguacgu guaccuggcc uuccaccaca ucaugaagua ccgggggaac      480

uuccuccacg agggcgagau caagaccaac gagcugcagg uccagcagau cgagcagcug      540

aaccaguucc ucaucaacaa guacaaggag aucuacgaca acgacgugga ggagcgcacc      600

uucaucgcga accaccagau cgacgcccug aaggagaucc ugcucaacaa gaacaccaac      660

aagacgacca aggagaagga gaucaagaag cuguucgacu acgccgacug cgagagccug      720

gagcccuaca ucuccugcuu cagcaagcuc gccgucgggg gcaaguucga ccuguccaag      780

cugggguacg agggcaacau ccaguucagc gaccccuccu ucgaggagaa ggccagcauc      840

cuccaggacg acuuccggcu gaucgcgauc gccaaggaga uguacgacuu cgugauccug      900

aacgaccucc ugaaggacca gcccaaccug uccaccgcca uggugcacgu guacgagacc      960

caccgccagg accucaagaa gcugaaggac aucaucaucg acaucgacgc caacaagggc     1020

cugcagaaca acgaccggug guacuucaag cuguucaagg acaagaacgc cccgaagaac     1080

uacagcgcgu ucauccacaa gaacuccaac cagaagcgcc ccagcaucga cgaguucaac     1140

gacgagauca agaagauccu gaucgagcac cggaacgacg ucucccugga gaagcaggac     1200

gaccucaaga ugcuguucga gaagguggag aacaagacgc ugcucaagac caucgccauc     1260

cagagcacca gcgugauccc ccaccagcug caccuggacg agcucgagaa gaucaucgac     1320

aacgccucca ccaaguaccc cuuccugagc aacauccgcg acaagcuggu ccuccuguuc     1380

acguuccggg ugccguacua cuacgggccc cugaacgaca agucccccua cagcaacgug     1440

gagcgcaagc aguucgccac cgucaccccc uggaacuucg acgagaucuu cgacgagaac     1500

aagacccgcg agaaguucau gaagaagcuc acgaacaacu gcaccuaccu guccggcgag     1560

accgugaugc cgaagaaggc ccuggcguac gaggacuacc ugaucuacga ccggcucaac     1620

gugcugcugg ucaacgggga guaccucagc cugcaggaga aggagaagau cuaccacuac     1680

cugaucuccc gccccaagac cacgcucgac cagcugaaga aggagcuggc caaguggcac     1740

aaccagagcc ccaucgacau caucuucucc aacauccacg aggagauccc cuucgaggcc     1800

agcucccacg cccaccucau gaaguccuuc gaccuggaga agcagaagga ggagcuggag     1860

cgguacauca cccucgccac caucuacgcg gacgacaaga agucccugaa ggagcugcuc     1920

aagaacgaga gccugcugga cgagaaccag aagaacguga uccucacccu gccgacgaag     1980

aagugggcgc cccugagcua caagcuccug cagaacaucg uguacaccga cgacuacggc     2040

gucgugcaca gcauccugga caucaugaag gagaccaacc agaacuucca gauggugcuc     2100

cacaacgagc aguaccacuu caaggagcug aucgagcagg agaaccagaa guucacccag     2160

gagaagacga ucgaggaccu gaucgaggag aagcucgagu ccauccccag ccugacccag     2220

cgcuccaucc ggcagacccu gcucaucauc gacgacauca ucaaggccag ccaccagaag     2280

guccccgaga agaucuucau cgaggugacc cgcaacaacg acgacaagcg gaagggccag     2340

gacgggaacc agcuggcccg cgacaaggag cugaaccggu uccucacgaa ccugcugaag     2400

gacgagaagg ccaaccaguu cugcgacguc agccagcucc ugaaggaccu ggaggaggug     2460

gacaagguga agcucaaggg caagcacauc uaccuguacu ucaagcagcu ggggaucgac     2520

cucuacaccg gcaagaagau cguccugaac cagcugcuca ccuccaccaa guacgacacc     2580

gaccacauca ucccgcagag ccugaucaag gacgacuccc uggacaaccu cgugcuggug     2640

gagcgcgagg ucaaccugaa ggugaagggg aaccacuacc ccgugcccag ccggauccag     2700

gacgagcaga agaagcucug gcacuuccug cugcagaaga aggccaucuc cgagaagaag     2760

uacggcaacc ucacgcgcac cagcaagauc aagcuggagg aggucgagga cuucguggcc     2820

cggcagauca acgugaucaa cuacuccaac gucaucaucc gcgacauccu gaagcucaag     2880

uacccccaca ccgacaucau cuucagcaag ucccaguacc cgagcuucau ccggaagcac     2940

cugaagaucg ugaagaaccg cgacgugaac gacgcccacc acgccgucga cgcguaccug     3000

aacguggugu ccggcaaccu ccugagcacc acguucucca aggccuacau ccggaagaag     3060

uacauccaga uccaggacgg ggagaaggag accuucaaca uggagaccau ccuggagaac     3120

uacuucaaga acaacgagaa ccagcucaag aucaagacca acugccugcg ccgggacgcc     3180

cugaucacgu ucaaggucga cuacuucagc ggcgaguucu acgggcagac ccgccucccc     3240

aagggcaacg agaaccugau ccccaucacc cugaacgagg acaaccccau guccgacacc     3300

agcaaguacg ggggcuacac gaaccucgcc caggccuacc uguucgcggu guccuaccag     3360

gaggacggcg agaugaagaa ggccauccug cgcgugccgu accucuacga cacccuguac     3420

aagaacgaca gcgacaagcu gcucagcaag guccuggaca accccaaggc caccaacauc     3480

cagauccugc ggaagaucca ccucaaccag aagauccgcu ucaacgacgg gaucuaccug     3540

cuguacacca acgagaagga ccggaacaag uacaagaugg ccuaccagaa cuacaucgac     3600

aacgacuacc ucuacuaccu gcacugggcc aacaagcacc uggaggagcu ccuggacaac     3660

caggagaagc agacguucau cuacaacucc aagaacgacc aguucgaggu gagcaaggcg     3720

aagaacaagg agaucuucca guaccugcuc cagaagacca aggccaaggu guacgacacg     3780

ugcaacuaca ucgucaaggc ccgcgagacc aacaucgaga ucuucgagca gcugacccug     3840

aaggagcaga ucaccaugau caaccagugg aucaagauga ugucccggga cugcgagaag     3900

gugaaguucg acgcccgcuu caccaacuuc agcaacugcc uccugcggcu guccaacaac     3960

aucaacccca aggacagcau caccaucauc uacgaguccc cgacgggccu cuucaagcac     4020

gaggugaaga ucuga                                                      4035


<210>  1838
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1838
augaagaaga ccaaccccua cauccugagc cucgacaucg gcaccgccuc caucggguac       60

gccugcaugg accuggacuu caacauccug aaguaccacg acaaggacgc gaucggcgug      120

cucacguucg agggggccga caccgccgcc gagcgccggg gcuuccgcac cagccggcgc      180

cggaagaacc gccggaucaa gcgccugggg cugcuccagg agguccuggc cccgcuggug      240

aagaacccca acuacuacca gcuccugaac cugcacaagu ggaagaacuc caacgaggac      300

uucaagcacc ggagccucuc cgaggugcug aagacccugg gcaagaaccc caagacguac      360

cccaccaucu accaccucca gcaggacauc cagaaccacc cggagaagaa guucgagccc      420

gagcugaucu uccuggcgcu cuaccaccug gucaaguacc gcggccacuu ccuguucaac      480

aaccucagcu ucgacaaggg gaagaccgug gacaacgagg cccagcuguc cgagcugcuc      540

gagcaguacc agagcaucaa ccccuacgag ggcuccaucu ucaacaagga caucaaggug      600

aucgucgaca cccugaagga cgaccagcug acgcggaacg acaagagcaa ggagcucaag      660

cgcaacgugu ccaagaccca cgccgagcug uucaagaugc ugguggggcu caaguucaag      720

cagagcaucc uguucgagaa cauggagaac gucgaggaca ucaaggaguc cgccaccggc      780

agcgacgcga ucucccugag cgacgacuac uacgagaacc ucuccgaguu ccugaccgac      840

gagcagcuga acuucaucga gagcgccaac acguucuuca uggggaucau gcucgaccgg      900

auccugaagg gcgccaccuc caucagcgcc gccaaggugc gcgacuacaa gaucuucaag      960

aaccagcuga agcucaucaa ggacaucgug uaccagaaca aguacgacaa guaccacgag     1020

uucuucguca ccagcaagga ggacaugcgg gcguaccagg agaaccccga cgccaagacc     1080

uucggcaagc uguccaaguu cgaccaguac cugauccacc cgaaggacca guacgccaag     1140

cucaugaagg accugaaggg gaucgugccc aagaaguaca cggaggugcu gaaccucauc     1200

gacgagcaga aguuccuggu cgugcagaac accaccgaca acgccagcau ccccuaccag     1260

aacaacgugu ucgaggccga gaacauccug cgcaaccagc agaaguacua ccccgagauc     1320

accgaggaga ugaucggcaa ggucaucaac cucaucuccu uccggauccc guacuacgug     1380

gggccccuga cgaacaagaa cggccagagc gacuucgcgu ggaugauccg caaguccgac     1440

gagcaggccc ugcccuggaa cuucgacaag gugaucgaca agagcaaguc cgccgagaag     1500

uucauccggc gcaugaccag caagugcacc uaccugcucg aggaggacgu ccugcccaag     1560

cacucccugc ucuaccagga gauggaggug cugaacgagc ugaacaacgu gcagcuccgg     1620

ggcgagaacg agccgaagca ccgcauccac cgccuggacc ccaaggucaa gcaguucauc     1680

uaccacaacc uguuccagaa gaagaagaac gugacccaca aggaccucau cacgcugcug     1740

cagaacagcg agcaccggaa cacccucaac aagaccuccg ggcagaagcu gaaggucuuc     1800

ggcacccaga acgacagcaa guucgccucc aagcugagca cguacaacga caugaaggcc     1860

aucuucgggu ccguggagaa caagcgccag augaucgagg agcucgugcu gugggucacc     1920

aucuucgagg acaaggcgau ccugaagcag aagaucggcg agaaguaccc caacaucacc     1980

gagaagcagc ucaaccagau cgugaagcug aacuacagcg gguggggccg gcuguccgag     2040

aagcuccuga ccaaggaggu gaacggccgc agccugaucg accucaugua cuccacggag     2100

aacaacuuca uggagaucau caccgacaag acccuggggu ucgacugcuu caucaagaac     2160

agcaacaagg ucaagaacgc caagaucagc uacaaggacg uggccgagcu gcggaccucc     2220

cccgcccuca agaagggcau cuggaacgcc aucaagaugg ugcgcgagau cagcaaccug     2280

uucggggcgc cggagaagau caucaucgag uucgccacgg aggaccaggu caagggcaag     2340

cggcagaagu cccgcagcga gcugugggac gaccucguga agaagaacaa ccugaagcgg     2400

aacaaggagu ucaaggggcu guucgaggag aucaaggccu accccgaccu cgacuucucc     2460

aaccccaagc uguggcugua cauccaccag aacggcaagu gcauguacac caagaagccc     2520

aucgaccucg agcgccugcu gagcgacacc aacaaccagc ucuacgagau cgaccacauc     2580

cugccgcgga ccuucgugaa ggacgacucc aucaacaaca agguccuggu gauccagagc     2640

gccaaccaga agaaguccgg cgacaagaug ccccucgaga ucuucgacaa ccacgugcug     2700

guccccuucu ggaagaugcu guacgagaac gaccucauca gcuccagcaa gcuguccaag     2760

cugaugaaga aggaguucaa cgaccucgac aaggaggggu ucauccagcg ccagcuggug     2820

gagacccggc agaucaucag ccacgugucc gacuuccuga cggaggagua ccccagcacc     2880

accgucgugc cgaugaaggc gaacgugguc uccgagcucc gccggaaguu cgacaucccc     2940

aagauccgcc agcugaacga cuggcaccac gccguggacg ccuaccugaa cggcuucgug     3000

uaccaggccg cccgggucuu cuaccccaag gugaaccucu ucgaguucaa cuucaagugg     3060

gagaaggugc gcgagaagug gcggcagcug ggggaguuca ccgagaacaa caagaagcgc     3120

gaguucuucc uguucaagcg cauggagaac uacgucaacg agcacggcga ggggaugauc     3180

agcaagcuca ucuucgaccu ggacaacuuc aaguucaacu acagccggaa gguggagucc     3240

acggagagcg cguucuacaa gcaguccgcc uucagcccga agaacaccac cgcccaguac     3300

gacuccgaca agaacaagac cgccaucuac aaggagauga agaacaagcg cggcaucgug     3360

gucagcuaca cguacgugaa gaaggaccag gagcugaucg gcuacggggu guucaacgag     3420

accgucuucg aguacgugaa caacgccgac guggacauga acacccgggc gcucaagauc     3480

guccgcaagg ccuuccccaa gcuggacauc aagaaggugc aguaccuguu cuccaucaac     3540

aagggcgaca cccuccuggu gaacgggcac cccuuccugu ucgucagcgc cuacgagcgg     3600

aucaacggca agcagcucga guucgacauc gccaugcagc gcaagaucaa gcaggcccug     3660

ucccccuaca acaacaugac ggagcaggag aagguggagu ucuacaacca guucgcggag     3720

aaggugaucg aggaguuccg gaacagcauc ggccugcagu cccgcgaccg gaaggucaac     3780

cucaucaaga accuguucaa caacaccgag aaguccaacg acagcaugac ccagagcauc     3840

cuggaggugc ucaagaugac caaggugucc gccgagcgca gcaaggagcu gaagauccgg     3900

uggaacgggc agacgccgaa caccuucauc gacaccaaga acaacgccga gauccaguac     3960

aaguccauca ccggccugaa gacgaccaag cccaagagca ucuucaagcu cgccgagucc     4020

aagaacgagc uguga                                                      4035


<210>  1839
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1839
augaagaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

gugaccgacg acuacaaggu gccggccaag aagaugaagg uccucgggaa cacggacaag      120

agccacauca agaagaaccu gcugggcgcg cuccuguucg acuccgggaa caccgccgag      180

gaccgccggc ugaagcgcac cgcccggcgc cgguacaccc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucag cgaggagaug ggcaaggugg acgacuccuu cuuccaccgc      300

cuggaggaca gcuuccucgu gacggaggac aagcggggcg agcgccaccc caucuucggg      360

aaccuggagg aggaggucaa guaccacgag aacuucccca ccaucuacca ccuccggcag      420

uaccuggccg acaaccccga gaagaccgac cugcgccugg uguaccucgc ccuggcgcac      480

aucaucaagu ucggcgggca cuuccugauc gagggcaagu ucgacacccg gaacaacgac      540

gugcagcgcc ucuuccagga guuccuggcc gucuacgaca acacguucga gaacuccagc      600

cugcaggagc agaacgucca gguggaggag auccucaccg acaagaucuc caagagcgcc      660

aagaaggacc gggugcugaa gcuguucccg aacgagaagu ccaacgggcg cuucgccgag      720

uuccucaagc ugaucguggg caaccaggcg gacuucaaga agcacuucga gcuggaggag      780

aaggcgcccc uccaguucag caaggacauc uacgaggagg agcuggaggu gcugcucgcc      840

cagaucggcg acaacuacgc cgagcuguuc cuguccgcca agaagcucua cgacagcauc      900

cugcuguccg ggauccucac cgucaccgac gugagcacga aggcgccccu guccgccagc      960

augauccagc gguacaacga gcaccagaug gaccuggccc agcucaagca guucauccgc     1020

cagaagcugu ccgacaagua caacgaggug uucagcgacg ucagcaagga cggcuacgcc     1080

ggguacaucg acggcaagac caaccaggag gccuucuaca aguaccugaa gggccuccug     1140

aacaagaucg agggguccgg cuacuuccug gacaagaucg agcgggagga cuuccuccgc     1200

aagcagcgca ccuucgacaa cgggagcauc ccccaccaga uccaccucca ggagaugcgg     1260

gcgaucaucc gccggcaggc cgaguucuac ccguuccugg ccgacaacca ggaccgcauc     1320

gagaagaucc ugaccuuccg gauccccuac uacgugggcc cccuggcccg cgggaagucc     1380

gacuucgccu ggcugagccg gaaguccgcg gacaagauca cgcccuggaa cuucgacgag     1440

aucguggaca aggagagcuc cgucgaggcc uucaucaacc gcaugaccaa cuacgaccug     1500

uaccucccga accagaaggu gcugcccaag cacagccugc ucuacgagaa guucaccgug     1560

uacaacgagc ugaccaaggu caaguacaag acggagcagg gcaagaccgc cuucuucgac     1620

gccaacauga agcaggagau cuucgacggc guguucaagg ucuaccggaa ggugaccaag     1680

gacaagcuga uggacuuccu cgagaaggag uucgacgagu uccgcaucgu ggaccugacc     1740

gggcuggaca aggagaacaa ggccuucaac gcguccuacg gcacguacca cgaccuccgg     1800

aagauccugg acaaggacuu ccuggacaac agcaagaacg agaagauccu cgaggacauc     1860

guccugaccc ugacccucuu cgaggaccgc gagaugaucc ggaagcgccu gaagaacuac     1920

uccgaccugc ucaccaagga gcagcugaag aagcuggagc ggcgccacua cacggggugg     1980

ggccggcuca gcgccgagcu gauccacggg auccgcaaca aggagucccg gaagaccauc     2040

cuggacuacc ucaucgacga cggcaacagc aaccgcaacu ucaugcagcu gaucaacgac     2100

gacgcccugu ccuucaagga ggagaucgcc aaggcccagg ugaucggcga gaccgacaac     2160

cucaaccagg uggucagcga caucgccggg ucccccgcca ucaagaaggg cauccugcag     2220

agccugaaga ucguggacga gcucgugaag aucauggggc accagcccga gaacaucguc     2280

guggagaugg cccgcgagaa ccaguucacc aaccagggcc ggcgcaacuc ccagcagcgg     2340

cugaaggggc ugacggacag caucaaggag uucggcagcc agauccucaa ggagcacccg     2400

guggagaacu cccagcugca gaacgaccgc cuguuccucu acuaccugca gaacggccgg     2460

gacauguaca ccggggagga gcuggacauc gacuaccuca gccaguacga caucgaccac     2520

aucauccccc aggccuucau caaggacaac uccaucgaca accgcguccu gaccagcucc     2580

aaggagaacc ggggcaagag cgacgacgug cccuccaaga acgugguccg caagaugaag     2640

uccuacugga gcaagcugcu cagcgcgaag cugaucaccc agcggaaguu cgacaaccug     2700

acgaaggccg agcgcggggg ccucaccgac gacgacaagg ccggcuucau caagcggcag     2760

cugguggaga cccgccagau caccaagcac guggcccgga uccuggacga gcgcuuccac     2820

accgagacgg acgagaacaa caagaagauc cggcagguca agaucgugac ccucaagucc     2880

aaccugguga gcaacuuccg caaggaguuc gagcuguaca agguccggga gaucaacgac     2940

uaccaccacg cccacgacgc guaccucaac gccgugaucg ggaaggcccu gcugggcgug     3000

uacccccagc ucgagccgga guucgucuac ggggacuacc cccacuucca cggccacaag     3060

gagaacaagg ccaccgccaa gaaguucuuc uacuccaaca ucaugaacuu cuucaagaag     3120

gacgacgugc gcaccgacaa gaacggggag aucaucugga agaaggacga gcacaucagc     3180

aacaucaaga aggugcuguc cuacccccag gucaacaucg ugaagaaggu ggaggagcag     3240

acgggcggcu ucagcaagga guccauccug cccaagggga acagcgacaa gcucaucccg     3300

cggaagacca agaaguucua cugggacacc aagaaguacg gcggguucga cagccccauc     3360

gucgcguacu ccauccuggu gaucgccgac aucgagaagg gcaagagcaa gaagcugaag     3420

accgugaagg cccucgucgg ggugacgauc auggagaaga ugaccuucga gcgcgacccc     3480

guggccuucc uggagcggaa gggcuaccgc aacguccagg aggagaacau caucaagcug     3540

cccaaguacu cccucuucaa gcuggagaac ggccgcaagc ggcugcucgc gagcgcccgc     3600

gagcugcaga aggggaacga gaucgugcug ccgaaccacc ucggcacccu gcuguaccac     3660

gccaagaaca uccacaaggu ggacgagccc aagcaccucg acuacgucga caagcacaag     3720

gacgaguuca aggagcugcu ggacguggug uccaacuuca gcaagaagua cacccucgcc     3780

gaggggaacc uggagaagau caaggagcug uacgcccaga acaacggcga ggaccucaag     3840

gagcuggcgu ccagcuucau caaccugcuc acguucaccg ccaucggggc ccccgccacc     3900

uucaaguucu ucgacaagaa caucgaccgg aagcgcuaca ccuccacgac cgagauccug     3960

aacgccaccc ugauccacca gagcaucacc ggccucuacg agacgcggau cgaccugucc     4020

aagcugggcg gggacuga                                                   4038


<210>  1840
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1840
auggagaacu acaacaucgg ccuggacauc gggaccacca gcgugggcug ggccgucgug       60

aacgacgcca cgaacaaggu gaugcgcaag ggcgggaagg cgcucugggg cguccggcug      120

uucgaccccg ccgugaccgc cgaggaccgc cgggccaagc gcgggacccg gcgccgguac      180

gaccgccggc gcgagcggau caagcugcuc caggacgagu ucaaggacga gaucaacaag      240

guggacucca acuucuuccu gaagcuggag gagagcaagu acaccuccaa ggacaucaag      300

aacaagacgg uccucaucag cauggaggag gagaaggcca ucaagaagua caaccaggag      360

uacccgacca ucuaccaccu ccgccagaag cugaugaacu ccaacgagca gauggacauc      420

cggcucgugu accuggcgau ccaccacauc aucaaguacc gcggcaacuu caucuacgag      480

gggaacagcu ucuccgugaa caaccugaac aucgacaaca agcucaagga gaucuuccug      540

agccuguccg aggacgucga cgagcucgac aucagcgagg agugcaucaa caacaucaac      600

uacaagcagc uggccaacau cuucaacgag caguccaaga acgaccugaa ggugaagcuc      660

accagcgagc ugaccuccua cagccugaac aagaacuuca ucuccgaguu cugcaagcuc      720

aucaucggca acaaguucag cgccaagaag cuguucaacc uggagacgga caacgacgug      780

ucccucagcu ucgacgggac cgacuucgag gacaaggccg gcgaccugga gucccugcuc      840

aacgacuacc uggagguccu ggagcagcuc aaggagcugu acgacauggu guuccugaag      900

aagaucuucg ggggcagcaa ggagaccacc aucagcggcc ucauggugga gcgguacaac      960

cagcacaaga aggaccugaa gcugcucaag gacacguucu ccagcgaccg caagaucuac     1020

aacaagaucu uccgguccaa gaaggacauc aaggacgaca aggacaugug ccuguacgac     1080

cuguacaucc acaacaagcu cgagaccacc gacuuccuga aggagaucaa gaaguacguc     1140

aacaccgaca acuucaucau cacggacgag gacuuccuga agcgccucga gaacaacgag     1200

gugcugcccc ggaucaccag caaggagaac gggaaguacc ccuaccagcu gaacaaggac     1260

gagcucgugu ccaucaucga gcaccagggc aagcacuacc ccuuccugcu gaacaagacc     1320

gacgacggga ucuacaagcu cguccgccug cuggaguucc ggaucccgua cuucgugggc     1380

ccccugguga gcaaggacau guccgagaac gcguggaugc uccgcaacga gggcaugcag     1440

gggguccaca ucacccccua caacuucaag aaggugauca accuggacaa gacggccgag     1500

aucuucauca agcgcaugau cagccacugc accuaccugc ucgacgagga ggcccugccc     1560

aacaacucca uccuguacag cgaguucaag gugcucaacg agcugaagca gaucaagguc     1620

aacggcgagc accugaccaa ggacgagcag cacaagauca ucgaggagcu guucaugaag     1680

accuccggga cgaucaccga gaacaaguuc cgggaguucc ugcugaacac ccucgacuac     1740

aacuucuacg agaacgagcu gaagaucacc ggcuacagcg ccgacugcaa guucgccaac     1800

aacaugcagu ccuacaucga cuucuucggg gagaacggca ucuucgaggg cacgagcuac     1860

aacaccaacg acgcggagaa gaucaucgag uggaucacca ucuucgacga caaggacauc     1920

cugaagcgca agguggagaa cgaguacccg gagcucaagu ccaaccaggu caagaacauc     1980

cugaacaaga aguacagcgg guggggccgg cugucccgca agcuccugcu ggaccuccgg     2040

gugcuggaca aggagaccga cauccccaag agcaucaugg accugaugua cgagacggac     2100

gagaacuuca ugcagaucau caacaacgac aaguacaacu uccagaagcu caucgccaag     2160

gagaacaaga ucgagaacac cuccaacgac cugagcuacg acguggucga gaaccuggcc     2220

accagccccg ccaccaagcg cgggaucuac caggcccuca agguggugaa ggagaucacg     2280

accuacaugg gcuacgagcc caagaacauc auggucgaga uggcgcgcgg ggacgagaag     2340

aagauccgga aggacgaccg gaagaaguac cugcagaagc uguacgacaa caucaaggac     2400

gagguggacu ccaacuacaa gaaccucaag aaggagcugg ccaaggagga gaagaucgac     2460

accgacaagc uguaccucua cuaccugcag gagggcaagu gccuguacug cggcaagccg     2520

aucaacgugg acgaccucaa caccaagaac agccuguacg agaucgacca cauccugccc     2580

cgcacccuca ucaaggacaa cuccugggac aaccgggucc uggugugccg cgggugcaac     2640

cagaagaagg ccgccagccu ggugcucccc aaggaguucc ggaacgacua cacgaagucc     2700

aucuggaucc gccugaagaa caacaagcug augagcaaca agaaguucua caaccucacc     2760

cggaaguccu acagcgagga ggacaucaag ggcuucauca accgccagcu ggucgagacc     2820

cggcagauca ccaagcacgu ggccaacauc cugaacugcu ucuaccccaa gacgaagauc     2880

guguaccuca aggcgaacau cucccacagc uaccgcgaca aguacgaccu guucaaguuc     2940

cgggacauca acgacuacca ccacgcccac gacgccuacc uggccgccac ccucgggucc     3000

uaccaggaga aguaccugca cuacgaccug gacuacaaca agaucaacga guucaacgag     3060

aagcucauca agaacgagga cuacaagcgc cugcacuacg gcaucgucgu gaacagccug     3120

gacgaggagu uccaguccgc guucgccgag aucgccccgc gccucaucaa cgaggagacc     3180

ggggagcugc uguucaacgc caagaaguuc aacgaccucg uggaggacac ccuguaccgg     3240

aacgacaucc ugaucagccg caagacggag auccggaccg gcaagcucua caaggagacc     3300

aucuacaagc gcggcaaggg gaacaucaag aucaaggaga acaugcccac cgagacguac     3360

ggcggguacu ccaacaugga gaccagcuac cugacccugg ucaaguacgg caacaagacc     3420

aagcucaucg gcauccccau guccaucgcg aacaacaagg acgugaaggu gaagcuggac     3480

uucaucaagg agcaccugga ccucaagagc cuggacaaca ucgucauccu gaaggacaac     3540

auccccuacg acgugagcau caacuacaag aaccaggacg uguacgucaa gggguacucc     3600

acggccaaca agaacugcga gcucagcaac gcccaccagc ugaagaucaa gaaggacaug     3660

augaagaagu gcaaguacgu gcugaacaag guguacaaga acaaguacga cgacaucacc     3720

aacauggaca ucgagaacgc caucaccuuc aucaacuacc ucuucgagau caagaucgag     3780

uucccgcugu ucuccaagga gaucaccaag auccaggaca acaucaaccu gacggagugc     3840

agcuucgagc agcucgccaa caucaucaag cagcugcuga ucaucuacca cugcaacucc     3900

aagaacggca accucaagga guucgggcug agcgaccgga ucggccgccu guccggggcg     3960

aacgucaccg gcggcgacuu caucuucacc agcgugaccg ggcucaagga gaagacggcc     4020

uccuacgacg gcgaguga                                                   4038


<210>  1841
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1841
augguggaga aguacggcau cgggcuggac aucggcacca gcacccucgg guggacgucc       60

gucgacgaga acggcaagau ccagcgccug aaggggcaga ccgcccuggg cacccggcug      120

uucaacgagg gccagaccgc cgaggagcgc cggggguucc gcacgacccg gcgccggauc      180

cagcgccggc gcuggcggcu gcgccugcuc cgggagaucu ucgacgagcc caucggcagc      240

guggacgcgg gguucuucgc ccgccagaag auguccagcc uguccccgca ggacgaccgg      300

uucgaggaca ccuaccgccu guucaacaac cagaccgaca aggccuucca cgacgaguac      360

cccacgaccu accaccuccg gcaggcccug augaacagcg accgccaguu cgacauccgg      420

gagaucuacc uggccaucca ccacaucgug aaguaccgcg gcaacuuccu ccgguccggg      480

aagcccgcgg acuucaagac cggcaagcug gaccugaagg agaaguucga ggagaucaac      540

gccgacuggc agacccucau cagcgaggac gcgccccagc ugccggucga cgaccugacg      600

accaucaagg ccgugcucgu ggacggcucc cgcagccgcu ccgaccggca gcgcgggcug      660

gucaagcugu ggauggccaa cgagaccgug gagaagaacu ggaagcccgu gaugaccgag      720

cucgccaagg ccauccuggg ccugaagacg aagcucuacc ugcugaccgg gcucgaccug      780

cccaaggagg accagaaggc cuggaccuuc agccuggaca cccucgagga ccaccagggc      840

gacaucgacu ccuucuucga cgaccacgcc cagcagcuga ucgaccggcu ggcggagcuc      900

cgcgccauga uccagcugac ggagaucauc cccagcggcc ugaacuucuc ccagagcaug      960

guccagcggu acgaggccca ccgcucccac cucgagaugu acaagcagua cgcccuggac     1020

cagaccgacc cgaagcggac caaggcccug cgcgcggccu acaacgccua caucgaccag     1080

cacgagaucg ccgacgacuu cuacaccgcc gugaagcggg ugacgaagga cgaccagagc     1140

gagaccgcgg aggagaucaa ccgccucauc gggcuggagc aguucaugcc caagcagcgg     1200

accaaggaga acggcgucau ccccuaccag gugcaccagc aggagcugga ccagaucauc     1260

gcccaccaga agaaguacua ccccuuccuc gggaccgaga acccggugau cgaccaccgc     1320

gagucccagc ccuacaugcu ggacgagcug gucagcuucc gggugcccua cuacgugggc     1380

cccauggucg ccgccaaccc ggagcucaag acgaccgaga accccuucgc cuggaugauc     1440

cgcaaggcgc agggggagau cacccccugg aacuucgacg agaaggugga ccggaagggc     1500

agcgccaccg ccuucaucga gcgcaugaag acgaccgaca ccuaccugcu gggcgaggac     1560

gugcuccccc agcagucccu gcuguaccag cgguucaugg uccugaacga gcucaacaac     1620

auccgcaucg acgacgagcu gcugaccacg aaccagaagc aguggguguu cgaccgggug     1680

uucaagggga agggcaccau cagcgccaag caccuccagg acgcgcugaa gcaccacggg     1740

gagaccgacg gccugccgaa gaucuccggg cucgccgacc ccaccaaguu caacagcgcc     1800

cugaccuccu accgcgacuu ccagaagugg cugcccaacg acaucgaccg caaggaccgg     1860

acggccgaca ucgagaccau caucaacugg agcaccgucu ucgaggacac ccacaucuuc     1920

guggagaagc ucaaggagau cgaguggcug acggccgacc agcgccggcg caucgcgucc     1980

aaccgguacc gcggcugggg ccggcugagc aagcaccucc ugaccgagau caaggacgag     2040

caggggcugu ccaucaugga ccagcucugg cgcacccagc acaacuucau gcagaucguc     2100

cacgagcccg acuacgccaa gcagaucgag gccgccaaca ccgugggcau ggccaagcag     2160

gacaucggga cggugaucaa cgaccuguac accagcccgc agaacaagaa ggcgauccgg     2220

cagguccugg ccguggugaa ggacauccag aacgcccacc acggcgucgc ccccuccugg     2280

aucuucaugg aggccgcgcg cggggccgac cagaaccccc agcggagccg ccagcggcag     2340

cgccagcucc aggaccugua cgccaagacc gccgcggacc ugguggacac cgaggugcag     2400

caggagcuca cggacaagau caaggacaag gccaacuuca ccgaccggcu gaugcuguac     2460

uuccuccaga acggccagga cauguacucc aaggagaagc ugaacaucga ccgccugagc     2520

gaguacgaca ucgaccacau ccucccccag gcccugauca aggacgacuc ccuggacaac     2580

cggguccucg ugcaccagag caucaaccgc aacaaguccg acaccuucgc cagcgagaag     2640

uacggcgcca agaugcaggg ggcguggcac caguggcggg ccgccggccu gaucuccgcc     2700

cgcaagcugc ggaaccucga gaugcgcccg gacgagauca gcaaguacgc caccggguuc     2760

auccgccggc agcuggugga gacgcgccag gucaucaagc uggugaccga gauccucagc     2820

caccaguaca accccgagga gaccaagcug guguccguca aggcgggccu gagccacgag     2880

cuccggcgcg aggccggccu gcccaagcug cgggaccuca acgacuacca ccacgccauc     2940

gacgccuacc uggccgcgcg caucgggacc uaccugcuca agcgguaccc gcgccuggag     3000

cgguucuucg uguacggcca guuccagacg ucccgccugg accuccggcg cuucaacuuc     3060

auccacgacc uggucaagag caagcagggg gugaucgugg acuccgacac cggcgagauc     3120

cugugggacc ggcagaagga caucgacuac aucaagcacc ucauggggcu gaagcaccuc     3180

cucgugaccc acgaggucua cgacagcgcc gccgagaugu acgcccagac caucuucaag     3240

gcguccgaca acgacagcaa ggugcugauc cccacgaaga acgaccgccc caccgccauc     3300

uacggcggcu acaccaagcg gcgcaacggg uaccuggcca uccucaagau caccgagaag     3360

aaggccgaga cguaccgggu ggucggcgug cccacccugu acauguccca gcuggccacc     3420

gcgcgcagcc acuccgugga ggccgagaag gcccuccuca agagccugau ggccccggag     3480

auccuguccg agagcaaggu cguguccuuc gagguggucg ugagccacgu gcaccugaac     3540

cagcuggucc gggacaccgu guucgggccc gcccacuacu ucggccucgg gcccuuccgc     3600

uacgugcgca acuuccacca gcugguccug cccuucaagg accaggagau ccucgugaag     3660

cggacgccga ccgacgagga gcugaacacc guguucgagg acaucugcug gcaggucgag     3720

aacuacuucg cgguguucga caucaacggc uuccgcaacg cccugaagaa guccaucgac     3780

aaguucgagc agcuccccau cgagaacgug uucaaggaca agaagcuggu cacccagggc     3840

aagaaggagg ugcuggccuu cgugaugcag gggcuccacg ccaacgccag cuaccggaac     3900

cugaagguca gccugggcau caagacgccc cucgggcagc ugcagcugac caaguucaug     3960

cucucccgcg acgcggagcu gaucgacgag agccccaccg gccuguuccg gcgcauccgg     4020

cgccucaagg accuguga                                                   4038


<210>  1842
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1842
augggcaaca agcugcgcaa cguggacgac uacuaccucg ggcuggacau cggcaccgag       60

agcgucgggu gggccgugac cgacaaguac uacgugaucc ccaaguucaa gggcaacgcc      120

auguggggcg uccaccuguu cgacgagggg gagacguccg ugaagcggcg cggcuuccgg      180

agcgcgcgcc ggcgcaccca gcggaagcgc gagcggcucg cccugcugga ggcccucuuc      240

gacgaggaga ucugcaagaa ggacugcacc uucuuccgcc ggcugcacga guccgaccug      300

uggccggagg acaagagcgg ggacgacaag uacgccgugu ucgccgacga gaacuacacc      360

gacaaggacu uccacaagga guaccccacg aucuaccacc uccgcuacga gcugaucaag      420

aacaaggagc cccacgacgu caagcuggug uuccucgcgc ugcaccacau caucaagaac      480

cggggccacu uccuguucga cucccucagc uccgccgagg acuucaacag cgccuacgag      540

accaugugcu ccuaccugag cgacaacgag auccagcuga acugcuccga caucgagcag      600

uucaaggcca uccucaagga ccgccggcag gggaagaucg agaagaccaa gaaccuggcg      660

agccuguucg gcaucaccaa gaaguccgac cccgugagcu acgccgucau ggccgugcuc      720

uccgggagca cggugaagau gugcgaccug uucggcgacg acgagcugaa gaccgucgag      780

aagaacaaca ucuccuucgc cagcggcuac gaggagaacg aggagaucua cgccuccgug      840

cucggggacc gcuucgagcu ggugaagauc cugaaggcgg ucuacgacug ggccgugcuc      900

gccgacaucc uggacggcaa gaaguacauc agcgaggcca agguggccgu cuacgagaag      960

cacaagaacg accugaagac ccucaagaag uacgugaaga ccuaccugcc ggagaaguac     1020

aacgagaucu ucaagaucag cgacgggaag aaggacaacu acguggcgua cuccggccac     1080

aucaaggaga gcggcaagac gggggagcug aagaagaccu gcaacuccga gaacuucugc     1140

gccuaccuca agaagacccu gggcaagaac gccgaccuga gcuacaagga gauguucgac     1200

gagaucgaga acaagaccuu caugcccaag caggucacga aguccaacgg gguggugccc     1260

augcagguca acgaggagga gcucaaggcc auccuggaca acgccaagga cuaccugagc     1320

uuccucaacu ccugcgacga gaacggcgug agcgucuacg acaagaucau caagaucuuc     1380

gaguaccgga uccccuacua cguggggccg cugaacaccc acaccgagaa cuccuggcug     1440

aagcgcaagg acggcgugaa gaucuacccc uggaacuucg acgacgucgu ggacaucgac     1500

agcuccgcgg agaaguucau caacaaccuc accagcaagu gcacguaccu gcccgagugc     1560

gacgugaucc ccaagugcuc ccugcucuac agcaaguuca ccguccugaa cgagaucaac     1620

aaccugaagc ucaacggcga ggacaucccg guggagcuga agcaggggau cuacaaggac     1680

cuguucauga cccggcgcaa ggugaccguc aagcagauca aggacuaccu gaaggcccgg     1740

ggcugcuacg acaacaacga cgugcucucc gggaucgaca cgaccgucaa gagcucccuc     1800

aagagcuacu ucgacuucaa gggcuucgag ucccugaccu ucgacgacaa ggagagcauc     1860

aucgccagcu ccgugaucuu cggggacgac aagaagcucc ugaagaagcg ccugaagaag     1920

cuguuccccg agaagcugac cgacgaggac aucaagaaga ucugcaagcu gaaguacacg     1980

ggcuggagcc gccucuccaa ggaguuccug ugcgacgugg aguucaccua caagaagacc     2040

ggcgagguga ugaacgucau caccgcccug ugggagacga acuacaaccu caugcagauc     2100

cuguacagcg aggacuucug cugcgacggg gacgagaaga agaaccugaa cgacaagcuc     2160

gacgagcugu ccuacaccgg cgaccagcac agccugcgga aggccgugga cgcgcucuac     2220

guguccccca aggucaagcg ccccauccug cgggcccugc ucaucgccaa ggagaucgag     2280

aagauccagg agcgcccgcc caagaagauc uucguggagg uggcccgggg gaucgacaac     2340

cgcgaggagc ggcaccgcga cagccggaag aaccgccuga uggagcugua caagaacugc     2400

aagaaggagg cggagaaguu cugccccuac uucgacgccc ucuccaagaa gcugagcgac     2460

gaggacgagu ccaagcugcg gcgcgacaag cucuacuucu acuacaucca gcugggcaag     2520

ugcauguaca ccggggagga gaucagccug ggcgagcuca ugaacggcaa cgggcgguuc     2580

gacaucgacc acaucuaccc ccgcuccaag aucaaggacg acagccugac caacaagguc     2640

cuggugaaca agacggugaa caacaacaag accaacgucu acccgaucga cgccgccauc     2700

caguccaaga ugagccccua cuggcagaug cuccggucca gcggccugau cagcgaggag     2760

accuacaagc gccugacccg gaagacgccc cucaccgacg acgagcuguc cgccuucgug     2820

agccgccagc ugaccgagac ccagcagucc accaaggcgg uggcccacau ccucgagcag     2880

cuguacccca agccgaagac ggagaucguc uacgugaagg ccgcccuggu gcacgccuuc     2940

cggcagagca acgacaugcu caagugccgc gaggucaacg accugcacca cgcgaaggac     3000

gccuaccuga acaucguggu ggggaacgcc uacaacguca aggugaccca caacaaggug     3060

aacuucauca agggccucca gaacaacgac aagggcuaca ccgucaaccc cgagaaguuc     3120

uucucccacg acauccccgg ggccuggagc gugaaggacu ccaugaacac cgugcgccgg     3180

uucaugaaca agaacaacau ccgcuacacg cgguuccccc gcaagaagca gggcgggcug     3240

uucaagauca acccgcugaa gaagggcaac gggcaggucc cccucaaggc cagcggcgcg     3300

cgguccugca ucgagaagua cggcggguac gacaaggcca ccagcgccua cuucuccuac     3360

guggaguaca ccgacaagaa gggcaaggcc gugcgccaga uguuccccgu caaccuguac     3420

gaggugaagg aguacgaggc ggacaugcag ggguucguga ccagccggau cgccgaggcc     3480

uccaacguca agauccugau cccgugcgug aaguacgacg ccugcaucag cuucgacggc     3540

ugccgcaugu ucaucacgaa gaagaccggg aaccggaucg gcuacaagcc cggcaugcag     3600

cucgugcugg gguacaagua cgagaaguac auccgcaaca ucgagaacua ccugaccaag     3660

cacaaggacc gggagaucau cccccgcgac aacaucuccg ccgaggagaa caucgagcuc     3720

uacgacaucc ugaccggcaa gaugcugaac acgguccuca gcgugaaguu cuccgggauc     3780

ggcaagaagc uggagaagcg gcgcgagaag uucgugcugu gcagccccga ggagcagugc     3840

ugcguccuca acgagauccu gaagauccug cacgcgaacg ugcucuccgg ggaccugagc     3900

cugaucggcg agagcccgaa caccggcacc augaagacca acgccaaguu cuccgagauc     3960

aaggacgucc acagcaucaa gcucaucaac caguccguga cggggcuguu cgagcaggag     4020

aucgaccugc ucaaguga                                                   4038


<210>  1843
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1843
augggcaagc ccuacagcau cgggcuggac aucggcacca acuccguggg gugggccguc       60

gugaccgacg acuacaaggu gccggccaag aagaugaagg uccucggcaa cacggacaag      120

cagagcauca agaagaaccu gcugggggcg cuccuguucg acuccggcga gaccgccgag      180

gccacccgcc ugaagcggac cgcccgccgg cgcuacacgc ggcgcaagaa ccggcuccgc      240

uaccugcagg agaucuucac cggcgagaug aacaaggugg acgagaacuu cuuccagcgg      300

cuggacgaca gcuuccucgu ggacgaggac aagcgcgggg agcaccaccc caucuucggc      360

aacaucgccg cggaggucaa guaccacgac gacuucccca ccaucuacca ccuccggcgc      420

caccuggccg acaccuccaa gaaggccgac cugcggcugg uguaccuggc ccucgcgcac      480

augaucaagu uccgcgggca cuuccuguac gagggcgacc ugaaggccga gaacacggac      540

gugcaggccc ucuucaagga cuucgucgag gaguacgaca agaccaucga ggagagccac      600

cuguccgaga ucaccgugga cgcccugagc auccucaccg agaagguguc caagagcucc      660

cggcuggaga accugaucgc ccacuacccc acggagaaga agaacacccu cuucgggaac      720

cugaucgcgc ugagccucga ccugcacccg aacuucaaga ccaacuucca gcuguccgag      780

gacgccaagc uccaguucag caaggacacc uacgaggagg accuggaggg cuuccugggc      840

gaggucgggg acgaguacgc cgaccucuuc gccuccgcca agaaccugua cgacgcgauc      900

cugcucagcg gcauccugac gguggacgac aacuccacca aggccccgcu gagcgccucc      960

auggugaagc gcuacgagga gcaccagaag gaccucaaga agcugaagga cuucaucaag     1020

gucaacgccc ccgaccagua caacgccauc uucaaggaca agaacaagaa gggguacgcg     1080

agcuacaucg agagcggcgu gaagcaggac gaguucuaca aguaccugaa gggcauccuc     1140

cugaagauca acggguccgg cgacuuccug gacaagaucg accgggagga cuuccuccgc     1200

aagcagcgga ccuucgacaa cgggagcauc ccccaccaga uccaccucca ggagaugcac     1260

gccauccugc gccggcaggg cgagcacuac ccguuccuga aggagaacca ggacaagauc     1320

gagaagaucc ugaccuuccg cauccccuac uacguggggc cccuggcccg caagggcucc     1380

cgguucgccu gggccgagua caaggcggac gagaagauca cgcccuggaa cuucgacgac     1440

auccuggaca aggagaaguc cgccgagaag uucaucaccc gcaugacccu caacgaccug     1500

uaccugccgg aggagaaggu ccuccccaag cacagccccc uguacgaggc cuucaccgug     1560

uacaacgagc ugacgaaggu gaaguacguc aacgagcagg gcgaggccaa guucuucgac     1620

accaacauga agcaggagau cuucgaccac guguucaagg agaaccggaa ggugaccaag     1680

gacaagcucc ugaacuaccu gaacaaggag uucgaggagu uccgcaucgu caaccucacc     1740

gggcuggaca aggagaacaa ggcguucaac agcucccugg gcacguacca cgaccuccgg     1800

aagauccugg acaagagcuu ccuggacgac aaggccaacg agaagaccau cgaggacauc     1860

auccagaccc ucacccuguu cgaggaccgc gagaugaucc ggcagcgccu gcagaaguac     1920

uccgacaucu ucacgaaggc ccagcucaag aagcuggagc ggcgccacua caccgggugg     1980

ggccggcuga gcuacaagcu caucaacggg auccgcaaca aggagaacaa gaagaccauc     2040

cuggacuacc ugaucgacga cggcuacgcc aaccggaacu ucaugcagcu caucaacgac     2100

gacgcccugu ccuucaagga ggagaucgcg cgcgcccaga ucaucgacga cguggacgac     2160

aucgccaacg ucgugcacga ccugcccggc agcccggcca ucaagaaggg gauccuccag     2220

uccgugaaga ucgucgacga gcuggugaag gugaugggcc acaaccccgc caacaucauc     2280

aucgagaugg cgcgggagaa ccagaccacg gacaaggggc gccggaacag ccagcagcgc     2340

cugaagcucc ugcaggacuc ccugaagaac cucgacaacc ccgucaacau caagaacgug     2400

gagaaccagc agcugcagaa cgaccggcug uuccucuacu acauccagaa cggcaaggac     2460

auguacaccg gggagacccu ggacaucaac aaccugagcc aguacgacau cgaccacauc     2520

aucccccagg ccuucaucaa ggacaacucc cucgacaacc gcgugcugac ccgcagcgac     2580

aagaaccggg gcaaguccga cgacgucccg agcaucgagg uggugcacga gaugaagucc     2640

uucuggagca agcugcucag cgucaagcug aucacccagc gcaaguucga caaccugacg     2700

aaggccgagc ggggcgggcu caccgaggag gacaaggccg gcuucaucaa gcgccagcug     2760

guggagaccc ggcagaucac caagcacgug gcccagaucc uggacgagcg cuucaacacg     2820

gaguucgacg ggaacaagcg gcgcauccgg aacgucaaga ucaucacccu caaguccaac     2880

cuggugagca acuuccgcaa ggaguucgag cuguacaagg ugcgggagau caacgacuac     2940

caccacgcgc acgacgccua ccucaacgcc gucgugggca acgcccugcu gcucaaguac     3000

ccccagcugg agcccgaguu cguguacggc gaguacccca aguacaacuc cuaccgcagc     3060

cggaaguccg cgaccgagaa guuccuguuc uacagcaaca uccuccgcuu cuucaagaag     3120

gaggacaucc agaccaacga ggacggggag aucgccugga acaaggagaa gcacaucaag     3180

auccugcgga agguccuguc cuacccccag gugaacaucg ugaagaagac ggaggagcag     3240

accggcgggu ucagcaagga guccauccuc cccaagggcg agagcgacaa gcugaucccc     3300

cgcaagacca agaacuccua cugggacccc aagaaguacg ggggcuucga cagcccgguc     3360

guggccuacu ccauccuggu guucgccgac gucgagaagg gcaagagcaa gaagcuccgg     3420

aaggugcagg acaugguggg gaucaccauc auggagaaga agcgcuucga gaagaacccc     3480

gucgacuucc uggagcagcg gggcuaccgc aacgugcggc uggagaagau caucaagcuc     3540

cccaaguacu cccuguucga gcuggagaac aagcgccgcc ggcuccuggc cagcgcgaag     3600

gagcugcaga aggggaacga gcucgugauc ccgcagcgcu ucacgacccu gcuguaccac     3660

agcuaccgga ucgagaagga cuacgagccc gagcaccgcg aguacgucga gaagcacaag     3720

gacgaguuca aggagcuccu ggaguacauc uccguguuca gccggaagua cgugcuggcc     3780

gacaacaacc ucaccaagau cgagaugcug uucuccaaga acaaggacgc cgaggucagc     3840

ucccuggcca agagcuucau cucccuccug accuucacgg ccuucggcgc gcccgccgcc     3900

uucaacuucu ucggggagaa caucgaccgc aagcgguaca ccagcgugac cgagugccug     3960

aacgccaccc ucauccacca guccaucacg ggccuguacg agacccgcau cgaccugagc     4020

aagcucggcg aggacuga                                                   4038


<210>  1844
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1844
auggaccaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

gugaccgacg acuacaaggu gcccgccaag aagaugaagg uccucgggaa cacggacaag      120

cagagcauca agaagaaccu gcugggcgcg cuccuguucg acuccgggga gaccgccgag      180

gccacccgcc ugaagcggac cgcccgccgg cgcuacacgc ggcgccggaa ccgccuccgg      240

uaccugcagg agaucuucgc cgaggagaug aacaaggugg acgagaacuu cuuccagcgc      300

cuggacgaca gcuuccucgu ggacgaggac aagcggggcg agcgccaccc gaucuucggc      360

aacaucgcgg ccgaggucaa guaccacgac gacuucccca ccaucuacca ccuccggaag      420

caccuggccg acaucuccca gaaggccgac cuccgccugg uguaccuggc gcucgcccac      480

augaucaagu uccgggggca cuuccugauc gagggccagc ugaaggccga gaacaccaac      540

gugcaggccc ucuucaagga cuucgucgag guguacgaca agaccgugga ggagagccac      600

cuguccgaga ugacggucga cgcccugagc auccucaccg agaagguguc caagagccgc      660

cggcuggaga accugaucgc gcacuacccc gccgagaaga agaacacccu cuucgggaac      720

cugaucgccc ugucccucgg ccugcagccc aacuucaaga ccaacuucca gcugagcgag      780

gacgccaagc uccaguucuc caaggacacg uacgaggagg accuggaggg gcugcucggc      840

gagaucggcg acgaguacgc cgaccuguuc gcgagcgcca agaaccugua cgacgccauc      900

cuccuguccg ggauccugac cguggacgac aacagcacca aggccccgcu cuccgccagc      960

auggucaagc gcuacgagga gcaccagaag gaccugaaga agcugaagga cuucaucaag     1020

gugaacgcgc ccgaccagua caacgccauc uucaaggaca agaacaagaa gggcuacgcc     1080

ggguacaucg agaacggcgu gaagcaggac gaguucuaca aguaccucaa gggcauccug     1140

cugcagauca acggguccgg cgacuuccuc gacaagaucg accgggagga cuuccugcgc     1200

aagcagcgga ccuucgacaa cgggagcauc ccccaccaga uccaccucca ggagaugcac     1260

gccauccucc gccgccagga ggagcacuac cccuuccuga aggagaacca ggacaagauc     1320

gagaagaucc ugacguuccg gaucccguac uacgucggcc cccuggcccg caaggggagc     1380

cgguucgcgu gggccgagua caaggccgac gagaagauca cccccuggaa cuucgacgac     1440

auccucgaca aggagaaguc cgccgagaag uucaucaccc gcaugacccu gaacgaccug     1500

uaccuccccg aggagaaggu gcugccgaag cacagccugc ucuacgagac guucaccgug     1560

uacaacgagc ugaccaaggu caaguacgug aacgagcagg gcgaggcgaa guucuucgac     1620

gccaacauga agcaggagau cuucgaccac guguucaagg agaaccggaa ggucaccaag     1680

gacaagcugc ucaacuaccu gaacaaggag uucgaggagu uccgcaucgu gaaccugacg     1740

ggccucgaca aggagaacaa ggccuucaac uccagccugg ggaccuacca cgaccugcgg     1800

aagauccucg acaaguccuu ccuggacgac aaggccaacg agaagaccau cgaggacauc     1860

auccagaccc ugacgcuguu cgaggaccgc gagaugaucc ggcagcgccu gcagaaguac     1920

agcgacaucu ucaccaaggc ccagcugaag aagcucgagc ggcgccacua caccggcugg     1980

gggcggcugu ccuacaagcu gaucaacggc auccgcaaca aggagaacaa gaagaccauc     2040

cucgacuacc ugaucgacga cggguacgcg aaccggaacu ucaugcagcu gaucaacgac     2100

gacgcccuca gcuucaagga ggagaucgcc cgcgcccaga ucaucggcga cgucgacgac     2160

aucgccaacg uggugcacga ccugcccggc ucccccgcga ucaagaaggg gauccugcag     2220

agcgucaaga ucguggacga gcucgugaag gucaugggcc acaaccccgc caacaucauc     2280

aucgagaugg cccgggagaa ccagaugacg gacaaggggc gccggaacuc ccagcagcgc     2340

cugaagcugc uccaggacag ccugaagaac cuggacaacc cggugaacau caagaacgug     2400

gagaaccagc agcuccagaa cgaccgccug uuccuguacu acauccagaa cggcaaggac     2460

auguacaccg gggagacccu cgacaucaac aaccuguccc aguacgacau cgaccacauc     2520

aucccccagg ccuucaucaa ggacaacagc cuggacaacc ggguccucac ccgcuccgac     2580

aagaaccggg gcaagagcga cgacgugccc uccaucgagg ugguccacga gaugaagucc     2640

uucuggagca agcugcugag cgugaagcuc aucacgcagc gcaaguucga caaccugacc     2700

aaggccgagc ggggcgggcu gaccgaggag gacaaggcgg gcuucaucaa gcgccagcuc     2760

guggagaccc ggcagaucac caagcacguc gcccagaucc uggacgagcg cuucaacacg     2820

gaguucgacg ggaacaagcg gcgcauccgg aacgugaaga ucaucacccu caagagcaac     2880

cuggugucca acuuccgcaa ggaguucgag cuguacaagg uccgggagau caacgacuac     2940

caccacgccc acgacgccua ccugaacgcg guggugggca acgcccuccu gcugaaguac     3000

ccccagcucg agccggaguu cgucuacggc gaguacccca aguacaacag cuaccgcucc     3060

cgcaagagcg ccaccgagaa guuccuguuc uacuccaaca uccugcgguu cuucaagaag     3120

gaggacaucc agaccaacga ggacggggag aucgccugga acaaggagaa gcacaucaag     3180

auccuccgga aggugcugag cuacccccag gugaacaucg ucaagaagac ggaggagcag     3240

accggcgggu ucuccaagga gagcauccug cccaagggcg aguccgacaa gcucaucccg     3300

cgcaagacca agaacagcua cuggaacccc aagaaguacg ggggcuucga cucccccgug     3360

guggccuaca gcauccuggu cuucgcggac guggagaagg gcaaguccaa gaagcugcgg     3420

aaggugcagg acauggucgg gaucaccauc auggagaaga agcgcuucga gaagcacccc     3480

guggacuucc ucgagcagcg gggcuaccgc aacgugcgcc uggagaagau caucaagcug     3540

ccgaaguaca gccucuucga gcuggagaac aagcggcgcc ggcugcucgc cuccgcccgc     3600

gagcugcaga aggggaacga gcuggucauc ccccagcggu ucacgacccu ccuguaccac     3660

agcuaccaga ucgagaagaa cuacgagccc gagcaccgcg aguacgugga gaagcacaag     3720

gacgaguuca aggagcugcu cgaguacauc uccguguuca gccggaagua cguccuggcc     3780

gacaacaacc ugaccaagau cgagaugcug uucagcaaga acaaggacgc cgaggugucc     3840

agccuggcga aguccuucau cagccugcuc accuucacgg ccuucggcgc cccggccgcc     3900

uucaacuucu ucggggagaa caucgaccgc aagcgguaca ccuccgugac cgagugccug     3960

aacgcgaccc ugauccacca gagcaucacg ggccucuacg agacccgcau cgaccugucc     4020

aagcugggcg aggacuga                                                   4038


<210>  1845
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1845
augaagaagg aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccgccaag aagaugaagg ugcucgggaa cacggacaag      120

cagagcauca agaagaaccu gcugggcgcg cuccuguucg acuccgggga gaccgccgag      180

gccacccgcc ugaagcggac cgcccgccgg cgcuacacgc ggcgcaagaa ccggcuccgc      240

uaccugcagg agaucuucac cgaggagaug aacaaggucg acgagaacuu cuuccagcgg      300

cuggacgaca gcuuccucgu ggaggaggac aagcagggcu ccaaguaccc gaucuucggc      360

acccugaagg aggagaagga guaccacaag aaguucaaga ccaucuacca ccuccgcgag      420

gagcucgcca acagcaagga gaaggcggac cugcggcugg uguaccucgc ccuggcccac      480

augaucaagu uccgcgggca cuuccuguac gagggcgacc ucaaggccga gaacacggac      540

guccaggcgc uguucaagga cuucguggag gaguacgaca agaccaucga ggagucccac      600

cugagcgaga ucaccgugga cgcccucucc auccugaccg agaaggucag caaguccagc      660

cggcuggaga accucaucgc ccacuacccc acggagaaga agaacacccu guucgggaac      720

cugaucgccc ucucccuggg ccugcagccc aacuucaaga ccaacuucca gcucagcgag      780

gacgccaagc ugcaguucuc caaggacacc uacgaggagg accuggagga gcuccugggg      840

gagaucggcg acgaguacgc ggaccuguuc gccagcgcca agaaccucua cgacgccauc      900

cugcuguccg gcauccucgc cguggacgac aacacgacca aggcgccccu gagcgccucc      960

auggugaagc gcuacgagga gcaccagaag gaccugaaga agcucaagga cuucaucaag     1020

gucaacgccc cggaccagua caacgccauc uucaaggaca agaacaagaa gggguacgcc     1080

ggcuacaucg agagcggggu ggagcaggac gaguucuaca aguaccugaa gggcauccug     1140

cucaagauca acggcagcgg ggacuuccug gacaagaucg acugcgagga cuuccugcgg     1200

aagcagcgca ccuucgacaa cggcuccauc ccccaccaga uccaccucca ggagaugcac     1260

gcgauccugc ggcgccaggg ggagcacuac cccuuccuga aggagaacca ggacaagauc     1320

gagaagaucc ugaccuuccg gauccccuac uacgugggcc cgcucgcccg caaggggagc     1380

cgguucgccu gggccgagua caaggccgac gagaagauca cgcccuggaa cuucgacgac     1440

auccuggaca aggagaaguc cgcggagaag uucaucaccc gcaugacccu gaacgaccuc     1500

uaccugcccg aggagaaggu ccugcccaag cacagcccgc ucuacgagac cuucacggug     1560

uacaacgagc ugaccaaggu gaaguacguc aacgagcagg gcgaggccaa guucuucgac     1620

accaacauga agcaggagau cuucgaccac guguucaagg agaaccgcaa ggugaccaag     1680

gacaagcugc ucaacuaccu gaacaaggag uucgaggagu uccggaucgu caaccugacg     1740

ggccucgaca aggagaacaa ggccuucaac uccagccugg ggaccuacca cgaccugcgc     1800

aagauccucg acaaguccuu ccuggacgac aaggccaacg agaagaccau cgaggacauc     1860

auccagaccc ugacgcuguu cgaggaccgg gagaugaucc gccagcggcu gcagaaguac     1920

agcgacaucu ucaccaaggc gcagcugaag aagcucgagc gccggcacua caccggcugg     1980

gggcgccugu ccuacaagcu gaucaacggc auccggaaca aggagaacaa gaagaccauc     2040

cucgacuacc ugaucgacga cggguacgcc aaccgcaacu ucaugcagcu gaucaacgac     2100

gacgcccuca gcuucaagga ggagaucgcc cgggcccaga ucaucggcga cguggacgac     2160

aucgcgaacg ugguccacga ccugcccggc ucccccgcca ucaagaaggg gauccugcag     2220

agcgugaaga ucgucgacga gcucgugaag gugaugggcc acaaccccgc caacaucauc     2280

aucgagaugg cccgcgagaa ccagacgacc gacaaggggc ggcgcaacuc ccagcagcgg     2340

cugaagcugc uccaggacag ccugaagaac cuggacaacc cggucaacau caagaacgug     2400

gagaaccagc agcuccagaa cgaccgccug uuccuguacu acauccagaa cggcaaggac     2460

auguacaccg gggagacccu cgacaucaac aaccuguccc aguacgacau cgaccacauc     2520

aucccccagg ccuucaucaa ggacaacagc cuggacaacc gggugcucac ccgcuccgac     2580

aagaaccggg gcaagagcga cgacgucccc uccaucgagg uggugcacga gaugaagucc     2640

uucuggagca agcugcugag cgucaagcuc aucacgcagc gcaaguucga caaccugacc     2700

aaggcggagc gcggcgggcu gaccgaggag gacaaggccg gcuucaucaa gcggcagcuc     2760

guggagaccc gccagaucac gaagcacgug gcccagaucc uggacgagcg guucaacacc     2820

gaguucgacg gggcccagcg ccggauccgc aacgucaaga ucaucacccu caagagcaac     2880

cucgugucca acuuccggaa ggaguucgag cuguacaagg ugcgcgagau caacgacuac     2940

caccacgccc acgacgcgua ccugaacgcc gucgugggca acgcccuccu gcugaaguac     3000

ccccagcucg agccggaguu cguguacggc gaguacccca aguacaacag cuaccggucc     3060

cgcaagagcg ccaccgagaa guuccuguuc uacuccaaca uccugcgguu cuucaagaag     3120

gaggacaucc agacgaacga ggacggggag aucgcgugga acaaggagaa gcacaucaag     3180

auccuccgca agguccugag cuacccccag gugaacaucg ugaagaagac cgaggagcag     3240

accggcgggu ucuccaagga gagcauccug cccaagggcg aguccgacaa gcucaucccg     3300

cggaagacca agaacagcua cuggaacccc aagaaguacg ggggcuucga cucccccguc     3360

guggccuaca gcauccuggu guucgccgac gucgagaagg gcaaguccaa gaagcugcgc     3420

aaggugcagg acaugguggg gaucacgauc auggagaaga agcgguucga gaagaacccc     3480

gucgacuucc ucgagcagcg cggcuaccgg aacgugcgcc uggagaagau caucaagcug     3540

ccgaaguaca gccucuucga gcuggagaac aagcggcgcc ggcugcucgc cuccgcccgc     3600

gagcugcaga aggggaacga gcuggugauc ccccagcgcu ucaccacccu ccuguaccac     3660

agcuaccaga ucgagaagaa cuacgagccc gagcaccggg aguacgucga gaagcacaag     3720

gacgaguuca aggagcugcu cgaguacauc agcguguucu cccgcaagua cgugcuggcg     3780

gacaacaacc ugaccaagau cgagaugcug uucagcaaga acaaggacgc cgaggucucc     3840

agccuggcca aguccuucau cagccugcuc acguucaccg ccuucggcgc cccggcggcc     3900

uucaacuucu ucggggagaa caucgaccgg aagcgcuaca ccuccgugac cgagugccug     3960

aacgccacgc ugauccacca gagcaucacc ggccucuacg agacccggau cgaccugucc     4020

aagcugggcg aggacuga                                                   4038


<210>  1846
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1846
augaaccaga ccaagaacua cgacaucggc cuggacaucg gggugggcag cgucgggugg       60

accaucacgg acucccagag ccagaaggug cucaagguga agggcaagca ggggaucggc      120

guccaccugu ucaacgaggg ccagaccgcc gaggagcgcc ggcaguuccg caccaaccgg      180

cgccggcuga agcgccggaa guggcgccuc cggcugcugc gcgagcuguu cgacgagccc      240

aucuccagca ucgacccgga guucuucgcc cggcugaagu acuucaacga guccagcaag      300

gaccccaaga acaaggggua ccagccccag cugcacgacg accucaagga cguggacuuc      360

uaccgccagu accccaccau cuaccaccuc cggcacgcgc ugaugacgca gaaggagaag      420

uucgacaucc gcgacaucua ccucgccauc caccacaucg ugaaguaccg gggccacuuc      480

cugaugaacg ggcacuccag cuccuucagc gucgcccaga ccgacuggaa ggccgaccug      540

accaagcugu ucgagcugau ccaggagacc cgcacgaacc uggacuucuc cuucgaccuc      600

accgagaucg acaccaucaa gagccugcug uucgacaaga accucucccg gaacgaccgc      660

cagaaggaga ucgugggccu gaucaacaac aacagccagc uggacaagaa ggacaaggcc      720

cucaccaagc agcuggugga guccgucgug aaggggaucg ugggcaugaa gacgcggacc      780

gacauccugc ucggcuacga ggucgacaac aaggagcaga gcaaggcgug gaacuucucc      840

aucgacgagc uggacgacca gauggccacc aucgacccgg cccugagcca gaacucccag      900

gcccacgaca ucaucacccu cauccagaag cuguacauga cggugcagcu ggcgcagauc      960

gugcccgagg ggcagagcgu cagcgagaug augaucaacc gcuacaaccu cuacgccgag     1020

cagcugcagc agcugaagga cuacaagaag aucaaccucg agaccggccu gcugcuccgg     1080

gaggccuacg acgagcugau gaccaaggcc cugaccgccg cgcccgggaa gacgauccac     1140

gacaccuucu acaacaaggu gaagaagauc cucaaggacc aggacaacaa gcaggcccug     1200

uccaucaagc aggccaucga ggacagcaac uaccugaucc cccuccgcau caaguccaac     1260

ggcgugaucc cguaccagcu gcaccagcgc gagcuggacg ccaucaucga gaaccagaag     1320

gaguucuacc ccuuccucgc cacccccaac cccgucgacg gccaccagga ggcgcugccg     1380

uacaagcugg acgagcugau cggguucaag gugcccuacu acgugggccc cauggucgac     1440

cccgaggugg acagcgacca gaacuucgcc uggauggugc ggaaggagaa gggggagauc     1500

accccgugga acuuccacaa caaggucgac aucacgcagu ccgccgacaa cuucaucaag     1560

cgcaugaaga ccaccgacac cuaccucauc ggcaaggacg ugcugcccca gaacagccug     1620

cucuaccagc gguacacggu ccugaacgag cugaacggga ucaagguggc caacaagaag     1680

cucaccaucg agcagaagca gcgccuguac gaccagcugu ucaagaagaa gaagaccgug     1740

aagaagcagg acauccucaa caaccugguc gccgcgggcg agauccacga ggacgacaac     1800

cugaccggcc ucuccaacgg gacgaccuuc aacaacaacc ugggcagcua caucgacuac     1860

ugcaagauca ucgggcagga caagcuggag gacgccucca agcaggccga caucgagcgg     1920

aucaucgagu ggagcaccac cuucgaggac gccaacaucu uccucacgaa gcuggccgag     1980

aucaacuggc ugaccgagga ccagcgccag aagcucuccc agauccggua ccgcggcugg     2040

gggcaguuca gcaagacccu gcugaccgac aucaaggaca acaaggacaa cuccaucauc     2100

gacaaccuca ugagcaccaa gaagguguuc augcagauca ucaacgacac gaccaucaag     2160

cagaagaucg acgacuacaa ccaguaccac cugaaccacg cggaccugaa ggacaccauc     2220

gccaaccucu ucaccagccc ccagaacaag cgggccauca accagacgcu gcuggugauc     2280

gaggacauca ucaacgccgu cggccacaag cccaaccgca ucuucaucga gagcgcgaag     2340

gacaaccgga acgccaagaa ccagcucacc cagucccgcc ugaagcggau cgugaagcag     2400

uucgaggaca acaagcagca cguggaccag agcgucuacg aggagcugaa gcagcucgac     2460

gacggcuccc acgccagcau cgacgaccgc cugcugcucu acuuccugca gaacgggaag     2520

gacauguaca ccggccagcc gaucgacauc agccagcugu ccaccagcca guaccacauc     2580

gaccacaucc ucccccaggc caaggugaug gacaacgccc uggacaaccg ggugcugguc     2640

uccagcaagg cgaacacgga gaaguccgac cgcuuccuca gcgagauccc ccaguuccag     2700

cagcgguccc ugugggccga gcuggagcgc augggguucc ucaccaaggc caagaaggag     2760

cggcugcuga ugaccagcga cgaguucaag cagaacuccg ugcgcuucau cgcccgccag     2820

cucguggaga cccggcagau caucaagcug gucaaccagc ugaucaucca ggagcacccc     2880

gaggugaagc ucgugaacau caaggccgac cugacgaacg cguuccgcaa gaaguucaac     2940

uucgucaaga accggaacgc caacgacuac caccacgccu ucgacgccua ccugaccagc     3000

cucaucggcu cccaccuccu gaagucccac ccgaaguggg agccccucuu cguguacucc     3060

gacuacgaca agaucaaguu cgacaacgcg ccccaggggg ugaacaucau gaaggaguuc     3120

gagcagagca agcuggccga cgagaacggc gagaucaucu ggaacaagga cgagaugaug     3180

gccuaccugg agcgccucua caacucccgg cagauccuga ucaccaagaa ggucuacgcc     3240

cgccacggca accuguucaa gcagaccauc uucaaggccg gggagagcga caaccucauc     3300

cccgugaaga acaaccgguc cgacaucgcg aucuacggcg gguacaagag caagcagguc     3360

agcuacuacg cccugaucuc ccugcacaac aagaagcgcc agaagcggcu cguggccgug     3420

uaccugaucg acauccagaa gcugcagaac cucaacgaga gcgacuacuu caagacgcug     3480

cacaccauca ucaaggagcg ccacaacauc aagccggagu ucgacauccu ggccgacaag     3540

aucuaccaga accagcucau caucgacaac aagaacggcu ccaaccggcc cuuccgcauc     3600

gagggcgacu ucaagaacga ccggcagcug uaccugcccc uccaguacca gcgcuggcug     3660

gccagcaagg agccgaccga cgagaagcug aaccggaucu ucgaccacau cugcgagcag     3720

cucgcggacu acuucccccu guucgccggg aacgccugcg acaagcugcu cgacaaccgc     3780

gacaaguucu accagcuguc caucaagagc gaguucaacg acaagggcaa gcugaucgcc     3840

gaggggaagc ucuccgugau cgacaagcug cuggucgcca ugcacgcgga cagccggaac     3900

uccaaccucg ccgugaucgg ccugagcucc agcugggggc gcuucaagcu gcacggcggc     3960

aacgaggcca ucgggaccuc cgugcagcuc auccaccaga gccccacggg ccuguucaag     4020

cggauccagc gcuucaacug a                                               4041


<210>  1847
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1847
augcugagcg ccggcgugau gcucggggac gucuccgccc ucaagagcuc ccgcaacagc       60

cuguuccaga aguccaagaa gcugaucgag aacagcucca gcgaccugac caagguggac      120

uacuccaaga ugcgggagau ccugcucagc auccgcaaca ucgcgauccg guucuucaac      180

aacgaggacu ucaaggaguc cgacuacuac aaggaguucc ccaccaucug gcaccuccgc      240

aagaaccuga uggagagcga caagaaggcc gacauccggc ugguguaccu ggcccugcac      300

aacaucguca aguaccgcgg caacuuccuc caggagaaca acaaggaccu gcgggccgcc      360

aacgcgaacg ccggggccgc cgcgaucgac cuguccaacg cccucgagga guacuucagc      420

cugcacgagg acgaguccgc ccugacgugc gugccgaacg uggccgaccu ccagaugacc      480

cuggagcagc ccggccugcg caacggggag cggcucgacc gcaucgucgc cgcgcugaac      540

augcccgacc gggcccgcgc caagcuguuc gccaaggccu gcaucggcca gaagguggag      600

uucagcaagc ucgcgcuggg cauggagaag caggagggga ccaacgccua caucugggac      660

gacgacaagu acgacgagcu gguggcccuc ugccccgagg acuucuuccc gguccuggag      720

acccugaaga acgccuacuc cgccuacgug cucagcgacc ugcuggcggg caagcucucc      780

aucagcgcca gccugaucga cuccuacgag cagcaccaga acgaccugaa gaucgugaag      840

gagcucgucc gggaguaccu ggggcagaag gaguacggcc gccuguuccg ggggcccaag      900

uucgccaacg gcgacuacaa cuucaacaag guggccaagc ccagcuacac guccuacauc      960

cucggcgaga gccugugcaa caagcagggg accacccgcg aggagcuggu gaaggagcuc     1020

aagcgggucu ucgacgacug cuccgccauc caggcggaca agcgcuacca gaagaucaag     1080

cagcggcugg aggacgaccc caaguuccug aucaagcaga agacccgcgc caacggcgcc     1140

aucccguacc agcuccaccu ggaggagaug caccagauca ucgagaagca ggggaaguac     1200

uaccccuucc ugguggagca gcaggaccuc aucgagaagc uggugagcuc ccggaucccc     1260

uacuacgucg gcccccugaa cggcgggcac gacccgaacc accccuaccc cacgaacccc     1320

aucgacccga agcgcaaguu cgccuggagc gugcgcaagc ccggcaugga gcacgagaag     1380

gcguaccccu ggaacgugga cgaggucauc gacuccgagg agaccgccga gcgguucauc     1440

cgccggauga ccgggaccug cacguaccug uacggcgagc ccguggugcc gcgcugcagc     1500

cuccuguacg aggaguucug cguccugaac gagcucaacg gggcccggug gggcgagggc     1560

aacggggaca cccaccgcuu cgacuccagc gaccggaccg agauccugga gcagcuguuc     1620

aagaagcgca agcgggugac ccacaaggcc aucgccacgu ggcucgagau caaccacggc     1680

gucaccaacg ugaagaucuc cgggacccag gcggagaacg ccuucgagag caagcugucc     1740

agcuacaacg acuucugcaa ccugcucggc guggacgggc uggaggacgu cugcccccug     1800

accaaggacg acaucgagca gaucauccuc ugggugacgg uguucgagga caagaagcug     1860

cugcuccaga agcugcagcg cgccuucggc gacaagcugu ccgagaagca gaucgccagc     1920

aucaugcgga agcccuacgc cggcuggggg cgccucucca gcaagcugcu gaccggcauc     1980

aagcgggaga cccccuucgg gcgcaucagc auccucgacg ucaugcggga cggcgacccg     2040

cgccccgggc accaccugga cgcgaugaac uucauggaga uccugcggga gaaggacuuc     2100

ggcuuccagg accgcaucga cgagaucaac aaggagcgga ccaaggaguc cggccuccug     2160

cugagcgugg aggaccuccc cggguccccc gcccugcgcc gcagcgugaa ccaggccaug     2220

cacauccuga aggagaucgu cuccaucgcc ggcaaggagc cgagccggau cgugaucgag     2280

gugacgcgcg accccgacga ggagaagcgg gggcaccgca ccgacacccg guacaagcag     2340

cucuccgagg cccuggcggc caucaagacc gacaucgucc aguucgacag cucccugcuc     2400

gccgagcuga aggagcgcaa ggacagccug gacuccgagc ggcucaugcu guacuuccag     2460

cagugcggca agagccugua cuccggcaag ccccucgaca ucaaccgccu gagcgaguac     2520

cagguggacc acaucauccc gcagugcuac aucaaggacg acucccugga caacaaggcc     2580

cucguguucg cgagcgagaa ccagaucaag cuggacuccc ugcuccugga caagagcauc     2640

auccggagcc agaaggggcg cuggcaggag cugcaccggg ccggccucau cuccaacaag     2700

aaguuccgca accugacgug caccgagauc agcgaccgga ugaugaaggg guucaucaac     2760

cgccagcugg ucgagaccuc ccagaucgug aaguucgugc ggcagaugug cgagcagcgc     2820

uacgagagcu ccgaggucau cagccuccgg gccucccuga gccacaaccu gcgcgaccgg     2880

ugcggcuuca ucaagugccg cgagcucaac gacuaccacc acgcccacga cgccuaccug     2940

gcgugccaga ucgcccgguu caucgggauc cgcuaccccc gcuggcagga cggcaucucc     3000

cgggaguuca ucaacaagua caucaagcag cuggugaaga agggcaagag cuccgggaag     3060

uucuucgccg gcaagagcgg guucaucauc gacuccuuca ugcacgacgg cuucgaccgc     3120

gagaccgggg agaucuuccg ggacggcugg gacgccacgu ucgagaucac ccgcaugcgg     3180

cgcgugcucg gcuacaagga cugcuucauc acccggaugc ccgaggagca gaccggggcc     3240

uacugggagg agacgaucua cagcccccug gacgcgaaga acggcaagaa ccugcagauc     3300

ccgcucaagc aguccgggaa ggagggcuac cuggacccca agaaguacgg gggcaccaac     3360

accgccuuca acgccuacuu cuucgccuuc gucgccaagg acgcgcgcgg ccgggacaag     3420

uacuucuucg agggggugcc cguguaccug gccaagcagc ucaacgagca gccccagauc     3480

cuggucgacu ucgccaacag caucgccgcg accaagaagu gcuccaccgc caagauccug     3540

cgcagcaagg ugccgcuccg gcagaaguuc cugcuggacg gcacguccuu cuaccucggg     3600

ggccgcaccg ggaacaagaa cgugauccug agcggcgucg aguucgccug cggcaucgac     3660

auggccaaga ugaucgacac cgccaucaag caccccgagg agcugagcca gaaggagcuc     3720

aucuccauca uccagaugcu ggcggacacc aucgagcgga acagcgaccg ccaggccucc     3780

cugaucaacu uccggcgcuu cgacgacaag cuccggaaca ucgacgccau cgccgccagc     3840

cgcaucauca agaaccugcu gaacuccaug aacgggguga agcagagcug cgaccucucc     3900

gaccugggcg ggagcaagca guccggcuuc augcagagca acaucgcgac gcuguucgag     3960

cggaucaccu ggugcuacuc cagcaucacc ggcauguucg agcagcgcgu guccuucgag     4020

gaccucaaga acgggaucug a                                               4041


<210>  1848
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1848
augaagaagc gcaaccggga ggaguacuac gugggccugg acaucgggac cagcuccguc       60

ggcugggccg uggccacccc cgacuaccgc aucccgaagu uccgggggca caagaugugg      120

ggcagccgcc ucuucgcgga ggggaagcgg gccgccgagc gccggcaguu ccgcacgaac      180

cggcgccggc uggcccgccg gcgcgaccgg cugcagaucc ucgaggagcu guucgccgag      240

gagauggcga agguggacaa caccuucuuc augcgccuga aggaguccaa guucuuccuc      300

gaggacaaga ccgucaagga gaagcacauc cuguucagca ccgacaaguu cgacgccgac      360

cgguucuaca aggacaaccc cacgaucuac cacgugcgca agcggcugau ggaggaggag      420

ccccaggaca uccgcgagcu guuccucgcc gugcaccaca uccugaagua ccggggccac      480

uuccucuucg ccggcucccu ggagcagaac ggggacagca ucgaggagcu gguccgcacc      540

cucuucggca agcaggggcu ggcgauccug gacgacacca ucgacuccaa cgagauggug      600

aacauccuca agaacaacga gaagaccaag agcgaccgca agaaggccgu gcaggagcug      660

uggaaccccc ugaaggacaa ggucaagaag gcccaggccg ccgagauggc gggcguggug      720

cucgggcuga acuccgacau cagcaagcug uucgacaacu ccgacuacaa ggaccucaag      780

aucaacuuca gcuccggcga cuacgacgag aagcgggacg acuacgccaa cagcaucggc      840

gacgacaucg uccugcugga cacgaugaag gcccucuacg acacccugau ccugucccag      900

auccuccagg ggaagcagag caucuccgag gccaaggugg aggacuacaa cacccacaag      960

gagcagcugc gcgcccugaa ggcgauccuc aagccgcaca agcggcugua caacgagaug     1020

uuccgcgaga acaacaagaa ggaccugagc aacuacgugg ccuacaucuc cagcuacagc     1080

gagggcaagc gggucaaccg cgagggguuc uacaaguacg ugcaggccgu gcucaagcag     1140

gucgacgacu ccgacagcaa gcaggccauc cuggaggcca uccggcugga gaccuuccuc     1200

ccccugcugc gcgugaagga caacgcggug aucccccacc agauccacca ggccgagcuc     1260

gaggccaucc uggagacggc ccggaagaag uacaaguucc ugaacgacgu cuccgagggc     1320

gugagcguga aggacaagau cauccagcug uucaccuucc gcauccccua cuacgucggc     1380

ccgcucaacc cccacuccgg ggagaccgug gccgaccgga agcagggcac cggguggguc     1440

gugcgcaagc agcggggcaa gguguacccc uggaacuucg cggacauggu cgacgugaag     1500

gccagcgccg aggccuucau cacgaaccug accaacaagu gcaccuaccu caucggggag     1560

gacgugcugc ccaagcgcuc ccugcuguac agcgaguuca ugcuccugaa cgagcugaac     1620

augaucaagu acgacggcca cgagcucccg gucgagauca agcggcugua caucgagcag     1680

gucuucaagc aggagcacaa gaagcagacc aagggccgcg ugcuggacuu ccucaagcgg     1740

aacgggcuga ucacgggcuc cgcgaccgcc gugaucaccg ggauggacga cagcaucaag     1800

ggcgaccugc aguccuaccg cgacauggug cggaucuucg ggaccggcuu cgaccacagc     1860

auggccgagc gcaucauccg cugggugacc cucuucggcg aggccaagga cauccuggag     1920

aacaagaucc gggaggucua cgggaacucc aucagcgacu cccagaugcg cgccaucaag     1980

aagcugaagu acgcggacug gggccggcuc agccaggccu ucuucaucaa gaucaucggg     2040

ugcgacaagu ccggcgugga gcgcaacauc aucacgugcc ugcgggagag cucccagaac     2100

cugauggaga uccucagcga ccaggggacc uacaucaagc agaucaaggc cuggaaccac     2160

cagcacacca cgaacaugau ggacacccug gacuaccagc ugguggacga ccucuacguc     2220

gcccccagcg ugaagcgcuc caucuggcag agccugugca ucguggagga gcuggucaag     2280

aucaugggcc aggcccccaa gaagaucuuc guggagguga cccggaccaa ccaggcggag     2340

aagacgaaga aggacucccg caagaagcgg cucgucgagc uguacaccgc caucggcaag     2400

gagggggccg ccuggaagaa ggagaucgag aagcaccagg ccggcgacuu ccgccggaag     2460

aagcuguacc ucuacuacac ccagaugggg cgcugcaugu acagccacga gcccaucgcg     2520

auggacgccc uguucaccuc cgaguacgac aucgaccaca ucuacccgca gagccugacg     2580

aaggacgacu ccugggacaa ccucgugcug gucaagagcc agcugaacaa ggagaagucc     2640

gaccaguacc cccuccggcc cgccauccgc gagaagaaca aggccuacug gcaguaccug     2700

ugccgggcgg gcuucaucag cgagaagaag uacgagcgcc ugaugcggac caccgagcuc     2760

accgccgagg agcuguccgg cuucaucgcc cgccagcugg uggagaccag ccaggccacg     2820

aaggccguga ucgggcuccu ggagcggauc uacaagcaca ccgacaucug cuucgugaag     2880

gcggagaccg ucucccaguu ccgccaccag cacaacuuca ucaagauccg ggagcugaac     2940

aacuaccacc acgccaagga cgccuaccuc aacaucgugg ugggcaacgu cuaccacgag     3000

aaguucacca agaacccccg cgaguucauc gcccgcagca cggagcggcg cccguacaac     3060

cugaaccgga uguacgacuu cgaccugcuc aagcaggggc gcgugaucug gcaggucccc     3120

aagucccugg agaccgugca ggccaugaug aaguccaacg acgugcggau caccaagaag     3180

cugcucgugc agaagggcga gcuguacgac gcgaccauca cgaaggccca caagaccaag     3240

gaggggaugu acuucccccu gaagggcgcc gacgcccgcc uccgggacgu ccaccgcuac     3300

ggcggguacg agaagaucaa gaucgccuac uacagcgugu ucagcugcug cgagaaggcg     3360

agcgacacca uccacacccu gaucaucccc aucccggugu acgccgccgg ccugcuccag     3420

acggaggccg accugaccgc guacgccugg cagaccaagc agaagaccaa gcccaucaag     3480

gacaucuacu ccaugaucac gcccaucuac aagaagcugu gcaucaacac cgucgugaag     3540

aucgacgggu ucaaguacuu ccucggcggg cggaccgagg acuacaucug ccuguacccc     3600

gccaucccgg ugguccugga cgagcaggcc gccgcguacc ucaaggccgu gagcaaggug     3660

ggccgcgccg acgacgucga gaacaccgcc caggccaucg accugaucac ggcggaggcc     3720

aacgaggccu ucuacgacgu gcugcucaag aagauggagg ccaagaucuu cgugaaggcc     3780

uaccaggagc gggucgagaa gcuggcgucc gagaccgccc gcgagcgguu ccgcaagcug     3840

agcaucggcg cccagugcuc cugccuccug gagauccuga accuccuggc caacaacaag     3900

accaccagca accugaagaa guggcucggg gugacgaugu cccggcgcaa gcagcccaug     3960

gaccugacca agcuggagga gugcaucgug uacgagacca gcaucaccgg ccucuacgag     4020

aaggccgucu cccugcugug a                                               4041


<210>  1849
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1849
augguggagc acuacgccau cggcuucgac aucgggaccg gcagcaccgg guucgccggc       60

gucgacccca acgggaagcu ggugcgcaug cggggcaaga acguguacgg cgucuaccgc      120

uucaagggcg gggaggcggc cgccgaccgg cgcggguucc ggacgacccg ccggcgcauc      180

aagcggcgca aguggcggcu ccgcuuccug aaguccuucu ucgagccgua caucgccccc      240

aucgaccccg aguucuuccu gcggcagaag uacagcgccg acagcccccu cgacccgaag      300

ggcauccgcg cggacaaggg gcugcugucc gacauguccg accaggaguu ccacgccaag      360

uaccccacca ucuaccaccu ccgguaccag cugaugcacg agaagcgcca guucgacgug      420

cgggagaucu accuggccau gcaccacauc aucaaguacc gcggccacuu ccucaucgag      480

gacggcgccg acaacuucga cgcgaagcag aucgacuucu ucgaccaguu ccagcggaug      540

gccgccguga ccgccgggcu gcccgagcug gagacgcucg ucgugcccga cgagacccag      600

agcgacgccu ucaagcgcgu gcuguucgac gagucccaga ccaagagcga ccggcagaag      660

guccuggcgu cccagcuccu ggacauccag gacaaccaga gcaaggccau caaggacuuc      720

aucgccgccc ugaccaaggc cguggugggc augaaggcga accucgccac gcugaccucc      780

accgccaucg agagcaccga caagaacgac uggacgaagc cgcuggggca gcucgacgac      840

uuccugcuga agcacgagga ggaccucgac gaggacggcu acgcccugau cgacgccgcg      900

aaccagaugu acuccgccgc cguccuggcc gcccucgugc ccgagggcau gggguucagc      960

gaggcgauga uggcccgcua cgcccagcac aagaagcagc uggcccugua caagcagguc     1020

aucgcguccc uccccgacaa gacccagcgc cacgagcuga aggacgccua cgccgacuac     1080

aucgagcacg ccgcccagaa cccccgggac gcguucuaca agcgcgugac caaggugcug     1140

gagggcgugg acagcgccga cgcccggacc gucaugacgg ccaucuccca cgacgaguuc     1200

cuccugaagc agcgcacccu gagcaacggg aagaucccgu accagaugca gcagcacgag     1260

uucgacgcca ucaucgagaa ccagaaggcg uacuaccccu ggcucgccac ccccaacccc     1320

guggaggccg acgccaagcg gcugccguac uaccuggacg agcucaucgg cuuccgcgug     1380

cccuacuacg ucgggcccau ggugaccgag aagcaguccc aguucgccug gaugauccgg     1440

aaggcggacg gcgagcugac gcccuggaac uucuucgacg uggucgaccg cgagaagacc     1500

gccaaccggu ucauccagcg caugcagacc accgacaagu acauccuggg cgaggacgug     1560

cucccgaagc acagcauccu guaccagcag uucgaggucc ugaacgagcu caacaacauc     1620

cggguggaga agcacccccu gacgaccgag cagaagcagu ucaucuacca gcgccuguuc     1680

guggaccagc agcggaagac caucaccaag aagaaccucc aggacacgcu ggucgccaug     1740

ggggaguucg acaaggcccc cgacaucggc gggcuggcga aggaggacgu gaugaugagc     1800

ucccuggcca ccuucgccga cuacagcaag auccuccccg aggccaucuc cgacccgacc     1860

cgccagaacg acaucgagga caucaucgcc uggagcacca ucuucgagga cacgcagauc     1920

uucuucgaca agcugaagac cguggacugg cugaccaccc agcagcggaa gcgccucgcg     1980

uccauccggu acacgggcug ggggaagcug agcaagcgcc ugcuccagca gauccccacc     2040

uccaagcaca ccaacgucau ggccgcccug ugggccacca gcgacaacuu caugcagauc     2100

cugcaggacg agcaguucca ggagggccuc uacgacgcca accggcugcc cgaggagacc     2160

cugacgcccg acgagcaggu cgagaagauc cucgacgagg cguaccuguc cccggccgac     2220

aagaagggcg cccgcaaggu gaucaaggug augaacgaca ucgugcgcgc caaccacggg     2280

gugcugcccg agcaggucuu ccucgagagc gccgacgacc cccagaagaa caagcggcgc     2340

uccgugagcc gguccaagca gcuggaggag aaguaccgcc aggcggccca gagcuucauc     2400

gacgaggccg ugaaggagga gcugaacgcc cagaucguca acuccgcgga guucaacgac     2460

cggcucgugc uguacuucac ccagaacggc cgcgacaugu acaccgggaa gcggcuggac     2520

aucgagaacc ucggcagcua ccagaucgac cacauccugc cgcagucccu ggugaaggac     2580

gacagccucg acaaccgcgc ccugaccgcc cgggacauca acgccgagau guccaaccag     2640

uucgucagcg agcgcuucgg gcugggcccc cgggccuucu ggcagcagcu caagaacaac     2700

ggccugauca gcgggcgcaa guuccggaac cuggugaugc agcgcgacga gaucgacaag     2760

uacgcgcccg gcuucaucaa ccggcagcuc guggagacgc gccagaucau caagaccguc     2820

gagcagauca uccaccggcu guggcccgac auccagaucg ugaccaucaa ggccaaccug     2880

acccaccagg ugcgcuccga gcucggguuc ccgaaggucc gggagcugaa cgacuaccac     2940

cacgccuucg acgcccugcu cgccgcgcgc aucggccagu accugcugga gcacuacgcc     3000

ggccucaagc ccuucuucac guacggggag uucaagcggg gcgacaccaa gaaguacggg     3060

accaccaacu uccugcgccg ccugacgaag gacaacaagg acaagaaggg cgagcuccug     3120

cccgagguga gcgacuggau cgacuccauc gacagcaucu acaacuacaa gaagaucccc     3180

guggucuggg agaacaccac caacaugucc gcccuguucg accagaccau cuacccgcgg     3240

cccgaccgcg acacgaccaa gcggaagcuc auccccacca agcuggacaa gcccaccgac     3300

aucuacgggg gcuacagcuc cgccaagacg gccuacaugg cggugaugcg cgugcagacc     3360

aagaagggca ccgaguaccc ggucuucaac auccccguga gcaucgccuc ccagcugaac     3420

cggaucaccg accagacgau gcucaagcag caggucaagc aguucaucau caacgccgag     3480

cagagcgccu ccaagaagaa gcuguacgag agcggggacg ugauccuguc ccgcguggcc     3540

cucggccagc gguucaccga caagaacuac ggggaggugu uccugaucag caacauggag     3600

guccagaacc cccagucccu guggcucggc cgcgcgagcc agcagcggcu gacccgcgag     3660

cccgagggga acgagaagcc ggagaccucc cuggccgaca ucuaccagca gauccucaag     3720

caggucgacc gguacuuccu ggccuucaac auccgccagc agcggcagaa gcugcucggc     3780

acgcuggacc aguucaccag ccugcccgug ggccgcguga acgagcagac ccggaccgac     3840

cagaaccagc gcagcaucau cgcccgggug augaucgugc ucgggaaggg cucccgcgag     3900

gagaaguucc ccgaguucgg gcugagcgcg cugggccggc ucguccgcac cgggggcauc     3960

acgcuguccg aggacgccca gaucaucuac cagagcccca ccggccuguu cgagcgccgg     4020

guggcccuca aggaccugug a                                               4041


<210>  1850
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1850
auggccaaga uccugggccu cgaccugggg accaacagca ucggcugggc cguggucgac       60

aucgagaaca acaacgaguu cacccugauc gagaaggggg ugcgcaucuu ccaggagggc      120

gugaagaucg agaaggggau cgagggcucc aaggcggccg agcggacgac cuaccgcagc      180

gcccggcgca ucaaguaccg gcgcaagcuc cggaagaucc agcugcuggg cguccucucc      240

gagaacgggu acugccccuu ccugagcaag gacgagcugg acuccuggcg cuacaagaag      300

guguacccga ccaacaagag cuuccucaac uggcagaaga ccaacgagau ccagcuggag      360

aaccccuacu ucuaccgggc ccugcucgug gagaagaagc ugaaccugaa caucgaggag      420

gaccgcuuca agaucggccg ggccuucuac caccucgcgc agcgccgggg guuccugucc      480

aaccgcaugg agggcacgaa ggagaacgac gggaccguca ucaagagcau caccgagauc      540

uccaccgcca agggcgacaa gacgcugggc caguacuucu acgagcggua caagaagggg      600

gagaagaucc gcgacaccua cacccaccgg gaggagcacu accucgagga guucgugcgc      660

aucugcgagu uccagcagcu ggacaacaag uucgccgaga aggugcggaa ggccaucuuc      720

uaccagcgcc cccucaagag ccagaagggc cucaucggga agugcgucuu cgagcccaag      780

aagucccggu gcagcguguc ccgcccggag uucgaggagu accgcaugcu gugcuuccug      840

aacaacauca agaucaagac ccccagcgac gagaaccucc gguuccugac ggacaacgag      900

aaggagaagc ugaagcccca guucuuccgc aaguccaagg accacuucga cuucgaggac      960

cucgugaagc agcuggcgcc caagaaccag uucaaguucu acaagagccg ggacauccug     1020

ccgggcaacu ggcuguucaa cuacuccaug aacaccaccg ucagcggcug ccccaucucc     1080

gcccgccuga aggaccuguu cggggagaac uucauggaca ucaaguucga cuacauccgg     1140

gagaaggacg gcaagaaguc cgugaucgac auccacgaca ucuggcacgu ccucuucacc     1200

uacgacagcg aggagaagcu gaaggaguuc gcccgcaacc ggcugaaccu cgacgacauc     1260

aagaucaagg aguuccagcg cauccagcug aagaaggagu acgccgcccu gagccucaag     1320

gcgauccgga agauccugcc cuuccugcgc gaggggcuca ucuacagcca cgccguguuc     1380

aucgccaaca uggagacggc cauccccucc gagaucugga acaacuacac caaccggaac     1440

aucaucaaga acgagaucua caacaucauc agcaaccaga ccgaggacaa gcaggucgug     1500

gacaucguga acggcaucau caaguccagc cgcaucgaga acuccgugug gagcaacgag     1560

gccguggagu ucuucaagaa ggaccugaac uccaagauca agaacuacua cgggaucaag     1620

accuacaacg acuucccgga ggagaagaag auccggaucg agacguacgc guucaaccug     1680

uucaagcagc agaugaugaa gaacuucggc cgcggcgagu ucgccgccgu ccagcggauc     1740

gacgagcgcc ucaagcuguu ccugaccgac aacuucaaca ucgaccccau ccuccugaag     1800

aagaucuacc accccagcgc caucgaggug uaccagcccc cggugcggaa gaacgacggg     1860

aagcuguacc ugggcucccc cauggucagc uccauccgca accccauggc caugcgggcg     1920

cuccaccagc ugcgcaagcu gaucaacgag cucauccgca aggagcugau cgacagcaac     1980

accaagguga acaucgagau ggcccgggag cugaugaacg ccaacgagcg caaggccuac     2040

cagaacuggc agaacgaccg ggagaagauc cgcaaggagu acgcccucaa gaucaagcag     2100

caccuggacg ucgacaagga gcccuccgac guggacaucc ugaaguacca gcucugggag     2160

gagcagaacc acaagugcau cuacaccggg aaggagauca acaucagcga cuuccugggc     2220

uccaacccga aguucgacau cgagcacacg aucccccgga gcaucuccuu cgacaacagc     2280

cagagcaaca agacccugug cgagaacuac uucaaccgcu ccgugaagaa gaaccggauc     2340

cccgcggccc uccccaacca cggggagauc aucgagcgcc uggagaacug gaagcugaag     2400

uacgaggagc ucgagaagca gauccaguac gccguccggc aggccaaggg cgcgggcgac     2460

aaggacagca aggaccgcgc cauccagaag cggcacaagc ugaaguucga guacgacuac     2520

uggaagaaca aguacgaccg cuucgugaug gaggacgugc cggagggguu caagaacucc     2580

cagcuggucg acaccggcau caucaccaag uacagccgga uguaccucaa cacgcuguuc     2640

gacaaggugu acaccgugaa ggggaacacc gucgccgacu uccgcaagcu guggggcauc     2700

caggacgagu acgagaagaa ggcccgggug aaccacuccc accacugcgu ggacgccauc     2760

accaucgcgu gcaucaccaa gagcaacuac gagaaccucg ccaaguucua ccacgacugg     2820

gaggagcugc acgucaaggg gaucgaccag cugccgccca ugaacaagcc cuggaagacg     2880

uucaccgagg acaucaaggc cauccaguac gagauccucg ugucccacua cacccccgac     2940

aaccugccca agcagagcaa gaagauccug cgcaagaagg gccggaucca gaagaacgag     3000

aacggcaacc ccaucuaccu caagggggac acggugcgcg gcucccugca ccagcagacc     3060

uucuacgggg ccaucgagaa gaccgagguc aacaagaagg gcgagccgga gaaggugauc     3120

aaguacgugg uccgcaagag ccuggacgug cucgacgacu ccagcgugaa gaacaucguc     3180

gacgacaucg ugaaggagau cgugcagaac gcccggauca aggagaagga cuucaucaag     3240

gagcuggaga uccagaagcg gaagcuggcg aaggccgagg agaacaaggu cgugcagauc     3300

caggccgaga ucgacaagcu caacaagcag gucgccgacc uguacaccau gcccaacaag     3360

aacggcaacc ccauccccau caagaaggug cgcaucuucc agaccuccgu gacgaacccg     3420

auccaccuga agcagcagcg cgacaagagc cagaagaccu ccaagcccca caaggaguuc     3480

uacuacgccg cgaacgacag caacuacaug auggccaucu acgaggggaa ggacgagaag     3540

ggcaagguga agcgggacuu caagcuccug aacaaccugg aggccgggga guucuucaag     3600

uacuccgucc acaaggaccu caaggagaag aaccuggaga acauccgcaa cgugaucccc     3660

gacaagguga gcgccggcaa gaucgagauc ccccugaagg cgaucaucaa gaccgggacc     3720

auggucaucc ucugggagaa cagcccggac gagguguggg accugacgau cuccgaggug     3780

gacaagcggc uguacaaggu cgugggcaug agcgugaacc gcaucaaguc cgacgucaag     3840

accuucgagu ucggcaacau cgugcuccgg cacaacaagg aggccagccc cgccacccug     3900

cugaagaucc aggacgggga guucaagucc gacgagaacu acaucgccca gcgcaagcuc     3960

agccacaacc aguucaacgc ccugguggag ggcgucgacu ucucccugag cgugcucggg     4020

gagaucaagc ggcugaagug a                                               4041


<210>  1851
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1851
augggcgagg acgggaagcc cgugcgcguc aagggcaaga ccgccaucgg ggcccggcug       60

uuccaggagg gccacguggc ggccgaccgc cggauguucc gcaccacgcg gcgccggcuc      120

aaccgccgga aguggcgccu gaagcugcuc gacgagaucu ucgacccgua caucaccccc      180

guggacagca ccuucuucgc ccggcugaag caguccaacc ugagccccaa ggacucccgc      240

aaggaguuca aggggagcau gcuguucccc gaccugaccg acaagcagua ccacgaggac      300

uacccgacga ucuaccaccu ccggcgcgcc cucaugaccg aggacaagaa guucgacauc      360

cggauggucu accuggccau ccaccacauc gugaaguacc gcggcaacuu ccugaacucc      420

gcgaccgugg acaccuucaa ggccagcaag gucgacuuca agaaguccuu caaccagcuc      480

aacgagcugu acagcgccau caaccccgag gacgccuucc agguggacau gaacaagucc      540

gacgagacgg gccggcuguu ccucgacagc uccauccgca aguucgacaa gcagaagcag      600

aucagcaagc ugauccccgu ggucguggag gacaaggagg cgaagaagcu gaacgggaag      660

aucgcccucg agaucaccaa ggccauccug ggcuacaagu ccaagcugaa cguggucacc      720

cagaccgagc ccgaggaccc gaagaagugg gccgugaagu ucgacgacga ggacaucgac      780

agcaagcuca acgacauccu gcccgagaug gacgagaacc agcaguccau cguggagauc      840

auccaggacc uguacagcca ggucacgcuc aaccagaucg ugcccaacgg gaugucccug      900

agcgacucca ugaucaagaa guacaacgac caccacgagc accugaagcu cuacaagaag      960

cugaucgaca acaucagcga caccaagaag cggagcgccc ugaagaccgc guacgacaag     1020

uacgugggcg acgacgggaa gaccaucgag caggacgagu ucuacuccaa cgucaagaag     1080

aaccucgacg acagcgaccu guccaagcag aucgccgacc ugaucgaggc cgagaaguuc     1140

augcccaagc agcgcacgag ccagaacggc gugaucccgc accagcucca ccagcgggag     1200

cuggacgaga ucaucgagca ccaguccaag uacuaccccu gguucgccga ggccaacccc     1260

aacaagcacg accugcaccu cgcgaaguac aagaucgagg agcugguggc cuuccgcauc     1320

cccuacuacg ucggcccgau gaucaccccc gaggaccagg agaaguccuc ccagaccauc     1380

uucgccugga ugaagcgcaa ggagcccggg cagaucaccc ccuggaacuu cgacgagaag     1440

guggaccgga uggagagcgc caaccgcuuc aucaagcgga ugacgaccaa ggacaccuac     1500

cugaucggcg aggacgugcu cccggaccag ucccugcugu acgagaaguu caagguccuc     1560

aacgagcuga acauggugcg cgucaacgac cacccccuga aggugagcga gaagcaggcc     1620

aucuaccggg accucuucga gaaccagaag cgcaucuccg ugaagaaccu gcagaacuac     1680

gucaagagca aguccgggcu gcccaccggc cccaagauca gcggccucuc cgacccggac     1740

cacuucaaca gcucccuggg gacguacaac gacuucaaga agauguucgg cgacaaggug     1800

gacgaccccg accugcagga cgacuucgag aagaugaucg aguggagcac caucuucgag     1860

gacaagaaga uccugcgggc gaagcucgac gagguggccu ggcuguccga cacccagaag     1920

aacgaguuca aggagauccg cuaccagggg uggggccggc ugagcaagaa gcuccugacc     1980

gggaucgucg acggcaacgg ccagcgcauc aucgacgagc uguggaacac gaacaagaac     2040

uucaugcaga uccagagcga cgacuccuuc gccaagcgga uccacgaggc caacgccaac     2100

cagaugaagg cgaccgacgu ggaggacgug cucgccgacg ccuacaccag cccccagaac     2160

aagaaggcca uccgccaggu cgugaaggug gucgacgaca uccagaaggc gaugggcggg     2220

guggccccca aguucaucuc caucgaguuc acccggagcg aggaccgcaa cccgcggcgc     2280

acgaucuccc ggcagcgcca gcuggagaac acccugaagg acaccgccca ggcccucguc     2340

aagagcauca accccgacau ccuguccgag cuggacaacg ccagcaaguc caagaagggg     2400

cucaccgacc ggcuguaccu guacuucacg cagcucggca aggacaugua caccgggaag     2460

cccaucaaca ucgacgagau cagcaacuac gacaucgacc acaucuuccc gcaggcguuc     2520

aucaaggacg acucccugaa caaccgcgug cuggugagca aggccggcaa caacggcaag     2580

uccgccaacg ugccccucaa gcuguucggg gccaagaugg gcggguacug gaagcagcug     2640

gccgacgcgg gccucaucgg gcagcgcaag cugaagaacc ugcagaccaa ccccgacacc     2700

aucagcaagu ucgccaugca cggcuucauc cggcgccagc ucgucgagac cucccaggug     2760

aucaagcugg uggccaacau ccugggcgac aaguaccgga acgacgacac gaagaucauc     2820

gagaucaccg cccgcaugaa ccaccagaug cgggacgagu ucggguucau caagaaccgc     2880

gagaucaacg acuaccacca cgccuucgac gcguaccuca ccgccuuccu gggccacuac     2940

cuguaccacc gguacaucaa gcuccgcccc uacuucgucu acggggacuu caagaaguuc     3000

cgggaggaca aggugaccau gcgcaacuuc aacuuccugc acgaccugac ggacgacaag     3060

caggagcgga ucgccgacac cgagaccggc gaggugaucu gggaccgcaa gcggagcauc     3120

cagcagcucc gcgacgucua ccacuacaag uucaugcuga ucucccagga gguguucagc     3180

cugaacaagg agauguucaa gcagaagcug uucccggcca cccucgcgaa gaaguccaag     3240

cugauccaga ucaagaagga caagcccgug gacaucuacg gcggguacag caacaacacg     3300

gacgccuacc ucaccgucgu gaagcugaag ggcagcaagg aggacaagua caagaucgug     3360

ggggucccca ugcgggcccu ggacaaccuc aagcacgccg agaaggagag cccccagagc     3420

uacaccgccg agcugcgcaa ggcgcuggag ccgcaguuca ccaagaucaa gaagaaccgg     3480

aagacgggcg agguguccaa gguggucacc aacuucgaca ucgugcucgg gcgccuguuc     3540

uaccggcaga uggugaucga cggcagcgag aagucccuga ucgccagcuc cgucuaccag     3600

uacaacgcca agcagcucgu gcugagcgac caggccaucc gcaccaucuc cgaccaggag     3660

ggcaucgacg aguucgagcu gagcucccag uacgacaagg uguacgacga gaucguccag     3720

aagguggaga agcuccugcc caucuuccag caccggcacu uccaggagcg ccugaacaac     3780

ggcaagagcc ucuucgugaa ccugcccguc cacgacgugu acgaggggaa caagcuggag     3840

caggucggga agaagcacau caucaacucc auccucggcg ggauccacgc caacgcggac     3900

cgccccgaca ucgaccuguu cggcaucaag aaccugggcc aguucagcuc caccagcggg     3960

aucaaccucu ccagcgacgc caucaucugc uaccagagcc cgacgggccu guucgagcgg     4020

cgcguggugc ugaaggaccu cuga                                            4044


<210>  1852
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1852
augaccaagc gcgaggagcc cuacaacgug ggccuggaca ucgggagcuc cagcgucggc       60

ugggccgugg uggacaacaa cuaccaccuc cugaacauca agaagaacaa ccuguggggc      120

gcccgccucu ucaaggaggc ggagaccgcc caggucacgc gggggcaccg guccaugcgc      180

cggcgcuacc ggcgccggcg caaccggcug aacuggcugg acgagcuguu cgccgacgag      240

cuggccaaga ucgacccgag cuuccugcuc cgcaugaaga acuccugggu gagcaagaag      300

gaguccaccc ggaagcgcga ccccuacaac cuguucaucg acgagaagua caacgacgug      360

gacuacuuca accaguaccc caccaucuuc caccuccgga aggagcucau caccgaggac      420

aagaaggucg acauccgccu gaucuaccug gccauccaca acaucaucaa guaccggggg      480

aacuucacgc ucgagaacca gaacuucgac aucagccagc uguccagcaa cuucucccag      540

cagaucagcg acuucuucgc gcuguucucc gacuucggcg ugaucaugcc cgaggacuuc      600

gacccggaca agaucagcga cauccuccug aaccccaacc ugucccccag cggcaaggug      660

uccgaggcca ucgccaccau cagccccaag accaacguca aggccaagau caagaucauc      720

cuccugcugc ucguggggaa caacggcgac cugaagaagc uguucgaccu cgagaccacg      780

gagaagaucg cggugaagcu guccagccgc cacaucgaca gcgagcugcc gaucauccuc      840

uccgagcuga acgagcagca ggagaacauc aucaccaucg ccaacagcau cuucgggucc      900

aucauccuga aggacuuccu cggcgacgag accagcaucu ccgccgccaa ggucaucagc      960

uucgaggacc acaagcagga ccugcagaag cugaagacca uguggcggga gacguccaac     1020

aaggaggccg ugaaggcggg gaagaaggcc uacgaggacu acaucggcca cgaggacagc     1080

gagaccuucu acaagaagau caagaaguuc cucgagaagg cccagcccgu ggaccuggcc     1140

aacaaggccc uggcggagau cgagcucgag aacuaccugc ccaagcagcg caaccggaac     1200

aacaccguca uccccuacca gcugaacgag aacgagcuca ucgccauccu ggaccaccag     1260

gagaaguacu acccguuccu gaaggagaac cgcgacaaga uccucucccu gcugaccuuc     1320

cgcauccccu acuacguggg cccccuccag gacagcaaca acaaccgguu cuccuggaug     1380

acgcgcaagg ccagcggggc cauccggccc uggaacuucu ccgagaaggu gaacgucgag     1440

cagagcucca acgacuucau caagcgcaug cggagcaccg acaccuaccu gaucggcgag     1500

ccggugcugc ccaagaaguc ccugaucuac cagugcuacg aggugcucag cgagcugaac     1560

aacacccgcg ucaaggacgg guccagcaac cccaagcggc uggacgugac gaucaagcag     1620

cgcaucuaca acgagaucuu caagaaccag aaguccguga gcgucaaggu gcuccagaac     1680

uggcugauca aggagagcua cuucaagucc cccgagauca gcggccuggc cgacaagaag     1740

aaguaccucu ccagccuguc caccuacauc gacuucaaga agaucuucgg ccagcgguuc     1800

gucgacgacc cggugaacag cccccagcug gaggagcucg cggaguggcu gacccuguuc     1860

gaggacaaga agauccuccu gaucaagcug cagaacucca aguacagcua cgaccaggcc     1920

accaucaaca agcucuccac gaugcgcuac caggggaccg gcaagcugag caagaagcug     1980

cucguggacc ugaagaccac caagaagucc aucggcaaga gcggggccga gucccugagc     2040

auccucgacc ugaugugguc cacgaaggac aacuucaugc agaucaucca cgacgccgac     2100

uacaccuucg agcagcagau caaggaguuc aacuacgaca ccgaggacga gcugacgccc     2160

cucgagaagg ucgccaaccu gcacgggagc cccgcgcuga agcggggccu caaccagagc     2220

aucaaggugg uggccgacau cgucaaguuc augggccacg acccggagaa gaucuucauc     2280

gaguucaccc gcuccgacga cuucagcaag cugaccaucu cccgguaccg ccggaucaag     2340

aagcaguacc uggagaucgc caaggccauc aagaagaucc ccgcggaguu caaggacauc     2400

aaggaguacc agacccagcu cgaggagaac aaggggaagc uggccagcga gcgccugaug     2460

cucuacuucc ugcagugcgg ccacucccug uacagcaaca agcccaucga ccucaacaug     2520

aucaacucca gcaaguacca cguggaccac auccugcccc aguccuacau caaggacgac     2580

agccuggaga acaaggcccu cgugcuggcc uccgagaacg agaacaagau cgacaacaug     2640

cugaucagcc acgacaucau cgccaccaac cucccgcggu ggcaggcgcu gaaggaccag     2700

aaccugaugg gguccaagaa guucgccgac cucacgcgca ccaccgucac cgagaaccag     2760

aagaagggcu ucauccagcg gcagcuggug cagaccagcc agaucgugaa gaacaucacg     2820

cugauccuca acgaccugua caagaacacc uccugcaucg agacccgcgc cacccugagc     2880

uccgaguucc gcaaggccuu cagcaacuuc gacgagacga ccuaccacua ccaguucccc     2940

gaguucguca agaaccggga cgugaacgac uuccaccacg cccaggacgc guuccucgcc     3000

ugcgugaucg gggaguacca gcugaagaag uaccccaagg acaaccugcg ccucgucuac     3060

gaccaguacu ccaaguuccu ggacagccug aagaaggaca cccggaagaa gaacggccgc     3120

augccgcggu acacccagaa cggcuucauc aucgggagca uguucaacgg caagacguac     3180

guggacgaca acggggagau caucugggac cagaagauca aggaguccau ccgcaagacc     3240

uucaacuacc accaguucaa cgugguccgg cagaccaucg agcagcacgg caagcuguuc     3300

aacgacacca uccagcccca cagcgaccgc uacaagcuga ucccccugaa gacgaaccgg     3360

gaccccgcca ucuacggggg cuacaacaac gacaacaacg ccuacuccgu ggugcucgac     3420

gucgacggca agaagaagau caacgggauc ccgauccgca ucgccaacca gcucaagagc     3480

gacgagcugg accucuccag cuggcuggag aacaacauca agcacaagaa gcccaugacc     3540

auccugaucg acaaggugcc caaguaccag cggaucauca acgaggagac cggcgaccuc     3600

cugaucaccu ccgcgaacga ggugaucaac aacguccagc uguuccuccc cagcauguac     3660

acggcccuga ucucccugcu cgacagcacc aagaccgaga uguacuccaa gcugcugagc     3720

aacuacgagg ccaacauccu caucgacauc uacgacuacc ugcugaccaa gcucaagaac     3780

aacuacccgc uguaccgcaa ggagugggcc aagcuggcgg agcaccggga cgacuucauc     3840

gaguccgacc ucgugacgca ggccagcacc cugcagcagc ugaucaaguu caugcacgcc     3900

gaccccucca acgugaaccu caaguucggg aacuucaagg gcaaccgcuu cggccggaag     3960

aacgggaaca ucaagcugag caagaccgac uucaucuacg agucccccac cggccuguuc     4020

aagagcauca agcacaucga cuga                                            4044


<210>  1853
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1853
augaaguccg agaagaagua cuacaucggc cuggacgugg ggaccaacag cgucggcugg       60

gccgugaccg acgaguucua caacauccuc cgcgccaagg ggaaggaccu guggggcgug      120

cggcuguucg agaaggcgaa gacggccgcc gacacccgca ccuuccggag cgggcgccgg      180

cgcaacgacc ggaagggcau gcgccuccag auccugcggg agaucuucga ggacgagauc      240

aagaaggucg acaaggacuu cuacgaccgc cuggacgagu ccaaguucug ggccgaggac      300

aagaagguga gcggcaagua cucccucuuc aacgacaaga acuucagcga caagcaguac      360

uucgagaagu uccccaccau cuuccaccuc cggaaguacc ugauggagga gcacgggaag      420

guggacaucc gcuacuacuu ccuggccauc aaccagauga ugaagcggcg cggccacuuc      480

cugaucgacg ggcagaucuc ccacgucacg gacgacaagc cgcugaagga gcagcucauc      540

cugcugauca acgaccuccu gaagaucgag cuggaggagg agcucaugga cagcaucuuc      600

gagauccugg cggacgugaa cgagaagcgg accgacaaga agaacaaccu gaaggagcuc      660

aucaagggcc aggacuucaa caagcaggag gggaacaucc ugaacuccau cuucgagagc      720

aucgugaccg gcaaggccaa gaucaagaac aucaucuccg acgaggacau ccuggagaag      780

aucaaggagg acaacaagga ggacuucguc cucaccggcg acagcuacga ggagaaccug      840

caguacuucg aggaggugcu gcaggagaac aucacccucu ucaacacccu gaaguccacc      900

uacgacuucg ugauccugca gagcauccuc aaggggaagu ccacccugag cgacgcccag      960

gucgagcgcu acgacgagca caagaaggac cuggagaucc ucaagaaggu gaucaagaag     1020

uacgacgagg acggcaagcu guucaagcag gucuucaagg aggacaacgg gaacggcuac     1080

guguccuaca ucggcuacua ccugaacaag aacaagaaga ucacggccaa gaagaagauc     1140

agcaacaucg aguucaccaa guacgugaag gggauccucg agaagcagug cgacugcgag     1200

gacgaggcgg ugaaguaccu gcugggcaag aucgagcagg agaacuuccu ccugaagcag     1260

aucuccagca ucaacagcgu caucccccac cagauccacc uguucgagcu cgacaagauc     1320

cuggagaacc uggccaagaa cuaccccucc uucaacaaca agaaggagga guucaccaag     1380

aucgagaaga uccggaagac cuucacguuc cgcauccccu acuacguggg gccgcucaac     1440

gacuaccaca agaacaacgg cgggaacgcc uggaucuucc ggaacaaggg cgagaagauc     1500

cgccccugga acuucgagaa gaucguggac cugcacaaga gcgaggagga guucaucaag     1560

cggaugcuga accagugcac cuaccucccc gaggagaccg uccugcccaa guccagcauc     1620

cuguacuccg aguacauggu gcugaacgag cucaacaacc ugcgcaucaa gggcaagccg     1680

cuggacaccg acgugaagcu caagcugauc gaggagcugu ucaagaagaa gacgaagguc     1740

acccucaaga gcauccgcga cuacaugauc cggaacaacu ucgccgacaa ggaggacuuc     1800

gacaacuccg agaagaaccu ggagaucgug agcaacauga aguccuacau cgacuucaac     1860

aacauccugg gggacaaguu cgagcucgag auggucgagg accugaucga gaagaucacc     1920

auccacaccg gcaacaagac gcugcucaag aaguacaucg aggagaccua ccccgaccug     1980

agcuccagcc agauccagaa gaucaucaac cugaaguaca aggacugggg gcgccucucc     2040

cggaagcugc uggacggcau caaggggcgc aacaagaccg acgacgugaa caccgugauc     2100

aacuucaugc ggaacagcuc cgacaaccuc augcagauca ucgagggcaa gaacuacagc     2160

uucaacgagu acaucgacaa ccugcgcaag aaguacaucc cccaggagau cuccuacgag     2220

gucguggaga accuguacgu gagccccucc gucaagaaga ugaucuggca ggucauccgg     2280

gugacggagg agaucaccaa ggugaugggc uacgacccgg acaagaucuu caucgagaug     2340

gccaagagcg aggaggagaa gaagaccacc aucagccgca agaacaagcu ccuggaccug     2400

uacaaggcga ucaagaagga cgagcgggac ucccaguacg agaagcuccu gacggggcug     2460

aacaagcucg acgagagcga gcugcgcucc aagaagcugu accucuacua cacccagaug     2520

ggccgggaca uguacaccgg ggagaagauc gaccuggaca agcuguucga cagcacccac     2580

uacgacaagg accacaucau cccccagucc augaagaagg acgacagcau caucaacaac     2640

cucgugcugg ugaacaagaa cgccaaccag acgaccaagg gcaacaucua ccccgucccc     2700

uccagcaucc gcaacaaccc ggacaucuac aacuacugga aguaccugau ggagaaggag     2760

uucaucucca aggagaagua caaccggcuc auccgcaaca cgccccugac caacgaggag     2820

cugggggugu ucaucaaccg gcagcucgug gagacccgcc agagcacgaa ggccaucaag     2880

gagcuguucg agaaguucua ccagaagucc aagaucaucc ccgucaaggc cagccuggcc     2940

uccgaccucc ggaaggacau gaacacccuc aagagccgcg aggugaacga ccugcaccac     3000

gcgcacgacg ccuuccugaa caucguggcc ggcgacgucu ggaaccggga guucaccucc     3060

aaccccauca acuacgugaa cgagaaccgc gagggcgaca aggugaagua cagccugucc     3120

aaguacuuca cccgcccgcg caagagcaag gggaagguca ucuggacgcc cgagaagggc     3180

cggaagcuga ucguggacac ccucaacaag cccagcgugc ugaucuccaa cgagagccac     3240

gucaagaagg gggagcuguu caacgccacc aucuccggca agaaggacua caagaagggc     3300

aagaucuacc ucccccugaa gaaggacgac cggcugaagg acgugagcaa guacgggggc     3360

uacaaggcca ucaacggggc guucuucuuc cucguggagc acaccaaguc caagaagcgc     3420

auccggagca ucgagcuguu cccgcugcac cuccugucca aguucuacga ggacaagaac     3480

acgguccugg acuacgccau caacgugcuc cagcugcagg accccaagau caucaucgac     3540

aagaucaacu accgcaccga gaucaucauc gacaacuuca gcuaccugau cuccaccaag     3600

agcaacgacg gcuccaucac cgugaagccc aacgagcaga uguacuggcg ggucgacgag     3660

aucagcaacc ucaagaagau cgagaacaag uacaagaagg acgccauccu gacggaggag     3720

gaccgcaaga ucauggaguc cuacaucgac aagaucuacc agcaguucaa ggccgggaag     3780

uacaagaacc ggcgcaccac cgacaccauc aucgagaagu acgagaucau cgaccuggac     3840

acgcucgaca acaagcagcu guaccagcug cucguggccu ucaucagccu guccuacaag     3900

accagcaaca acgcggugga cuucaccguc aucggccugg gcaccgagug cgggaagccc     3960

cggaucacga accucccgga caacaccuac cugguguaca aguccaucac cggcaucuac     4020

gagaagcgca uccggaucaa guga                                            4044


<210>  1854
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1854
augaagaagc ccuacaccau cgcccuggac aucggcacca acagcguggg gugggucgug       60

gugacggacg acuaccgcgu cccgaccaag aagaugaagg ugcucggcaa caccgagcgg      120

aagaccauca agaagaaccu gaucggggcc cugcuguucg acuccggcga cacggcggag      180

gggacccgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgcaagaa ccggcugcgc      240

uaccucaagg agaucuucac ggaggagaug gccaaggugg acgacggcuu cuuccagcgg      300

cuggaggaca gcuucuacgu ccuggaggac aaggagggca acaagcaccc caucuucgcc      360

aaccucgccg acgagguggc guaccacaag aaguacccca ccaucuacca ccuccgcaag      420

gagcuggugg acaaccccca gaaggccgac cuccggcuga ucuaccuggc cgucgcccac      480

aucaucaagu uccgcgggca cuuccucauc gagggcaccc uguccagcaa gaacaacaac      540

cugcagaagu ccuucgacca ccucguggac accuacaacc ugcuguucga ggagcagcgg      600

cuccugacgg aggggaucaa cgcgaaggag cugcucagcg ccgcccuguc caagagcaag      660

cgccuggaga accucaucuc ccugaucccg ggccagaaga agaccgggau cuucggcaac      720

aucaucgccc ugagccucgg ccugaccccc aacuucaagg ccaacuucgg gcuguccaag      780

gacgugaagc uccagcuggc gaaggacacc uacgccgacg accuggacag ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugac ggaguccgac gagaucaccc gggccccgcu gagcgccucc      960

auggucaagc gcuaccggga gcaccacaag gaccucguga cccugaagac ccugaucaag     1020

gaccagcucc ccgagaagua ccaggagauc uuccuggaca agacgaagaa cggguacgcc     1080

ggcuacaucg agggccaggu gagccaggag gaguucuaca aguaccugaa gccgauccug     1140

gcgcgccucg acggguccga gccccugcug cucaagaucg accgggagga cuuccugcgc     1200

aagcagcgca ccuucgacaa cggcagcauc ccccaccaga uccaccugga ggagcuccac     1260

gccauccugc ggcgccagga ggucuucuac cccuuccuga aggacaaccg gaagaagauc     1320

gagagccucc ugaccuuccg caucccguac uacguggggc cccuggcccg gggccacucc     1380

cgcuucgccu gggugaagcg gaaguucgac ggggccaucc gccccuggaa cuucgaggag     1440

aucgucgacg aggaggcgag cgcccagauc uucaucgaga agaugaccaa gaacgaccuc     1500

uaccugccca acgagaaggu gcugccgaag cacucccucc uguacgagac guucaccgug     1560

uacaacgagc ugaccaaggu caaguacgcc accgagggca ugacgcggcc ccaguuccuc     1620

agcgccgacc agaagcaggc gaucguggac cugcuguuca agaccaaccg caaggugacc     1680

gucaagcagc ucaaggagaa cuacuucaag aagaucgagu gcugggacuc cguggagauc     1740

accggcgucg aggacagcuu caacgccucc cuggggacgu accacgaccu gcucaagauc     1800

auccaggaca aggacuuccu ggacaacccc gacaaccaga agaucaucga ggacaucauc     1860

cugacccuca cccuguucga ggacaagaag augaucagca agcggcugga ccaguacgcc     1920

caccucuucg acaagguggu gcugaacaag cuggagcgcc accacuacac cggcuggggg     1980

cggcucuccg gcaagcugau caacgggauc cgcgacaagc agagcggcaa gacgauccug     2040

gacuuccuca aggccgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacucc     2100

gagcugagcu ucaucgacga gaucgcgaag gcccagguca ucgggaagac cgaguacucc     2160

aaggaccucg ugggcaaccu ggccgggagc cccgccauca agaagggcau cucccagacc     2220

aucaagaucg uggacgagcu ggucaagauc augggguacc ucccgcagca gaucgugauc     2280

gagauggccc gcgagaacca gaccaccgcg cagggcauca agaacgcccg gcagcgcaug     2340

cggaagcugg aggagacggc caagaagcug ggcagcaaca uccucaagga gcaccccgug     2400

gacaacuccc agcugcagaa cgacaagcgc uaccuguacu accuccagaa cgggaaggac     2460

auguacaccg gcgacgaccu ggacaucgac uaccugagcu ccuacgacau cgaccacauc     2520

aucccccaga gcuucaucaa gaacaacagc aucgacaaca agguccucac cucccagggg     2580

gccaaccgcg gcaagcugga caacgugccc agcgaggcca ucgugcggaa gaugaagggc     2640

uacuggcagu cccugcuccg cgcgggggcc aucagcaagc agaaguucga caaccugacc     2700

aaggccgagc ggggcgggcu gacgcagguc gacaaggccg gcuucaucca gcgccagcuc     2760

guggagaccc ggcagaucac caagcacgug gcgcagaucc uggacucccg cuucaacacc     2820

gaguucgacg accacaacaa gcggauccgc aagguccaca ucaucacgcu caagagcaag     2880

cugguguccg acuuccggaa ggaguucggg cuguacaaga uccgcgacau caaccacuac     2940

caccacgccc acgacgccua ccugaacgcc guggucgcca aggcgauccu cggcaaguac     3000

ccgcagcugg cccccgaguu cguguacggc gacuacccca aguacaacag cuucaaggag     3060

cggcagaagg ccacccagaa gaugcuguuc uacuccaaca uccucaaguu cuucaaggac     3120

caggagagcc ugcacgugaa cuccgacggg gaggagaucu ggaacgccaa caagcaccuc     3180

cccaucauca agaacguccu gagcaucccc caggugaaca ucgugaagaa gaccgagguc     3240

cagaccggcg gguucuacaa ggaguccauc cugagcaagg gcaacuccga caagcugauc     3300

ccccgcaaga acaacuggga cacgcggaag uacgggggcu ucgacagccc caccguggcc     3360

uacuccgugc ucgucaucgc gaagauggag aagggcaagg ccaaggugcu gaagccggug     3420

aaggagaugg ucgggaucac caucauggag cgcaccgccu ucgaggagaa ccccguggug     3480

uuccuggagg cccggggcua ccgcgagauc caggagcacc ucaucaucaa gcugcccaag     3540

uacagccugu ucgagcucga gaacgggcgg cgccggcugc uggccagcgc guccgagcuc     3600

cagaagggca acgagcuguu ccugcccguc gacuacauga cguuccucua ccuggccgcc     3660

cacuaccacg agcugaccgg gagcuccgag gacgugcucc gcaagaagua cuucguggac     3720

cggcaccucc acuacuucga cgacaucauc cagaugauca acgacuucgc cgagcgccac     3780

auccuggcga gcuccaaccu ggagaagauc aaccacaccu accacaacaa cagcgaccug     3840

ccggucaacg agcgcgccga gaacaucauc aacguguuca ccuucguggc ccugggcgcc     3900

cccgccgcgu ucaaguucuu cgacgccacg aucgaccgga agcgcuacac cuccaccaag     3960

gagguccuca acgccacccu gauccaccag agcgugacgg gccuguacga gacccggauc     4020

gaccuguccc agcucgggga gaacuga                                         4047


<210>  1855
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1855
augaaguccg agaagaagua cuacaucggc cuggacgugg ggaccaacag cgucggcugg       60

gccgugaccg acgaguucua caacauccuc cgcgccaagg ggaaggaccu guggggcgug      120

cggcuguucg agaaggcgaa gacggccgcc gacacccgca ccuuccggag cgggcgccgg      180

cgcaacgacc ggaagggcgu ccgccuccag auccugcggg agaucuucga ggacgagauc      240

aagaaggugg acaaggacuu cuacgaccgc cuggacgagu ccaaguucug ggccgaggac      300

aagaagguga gcggcaagua cucccucuuc aacgacaaga acuucagcga caagcaguac      360

uucgagaagu uccccaccau cuuccaccuc cggaaguacc ugauggagga gcacgggaag      420

gucgacaucc gcuacuacuu ccucgccauc aaccagauga ugaagcggcg cggccacuuc      480

cugaucgacg ggcagaucuc ccacgugacg gacgacaagc cgcugaagca gcagcucauc      540

cugcugauca acgaccuccu gaagaucgag cuggaggagg aguucaugga cagcaucuuc      600

gagauccucg cggacgugaa cgagaagaag accgagaaga agaacauccu gaaggagcug      660

aucaacgccc aggacuucaa caagcaggag ggcaagaucc ucuacuccau cuucgagagc      720

aucgucaccg ggaaggccaa gaucaagaac aucaucuccg acgaggacau ccuggagaag      780

aucaaggagg acaacaagga ggacuucgug cugaccggcg acagcuacga ggagaaccuc      840

caguacuucg aggaggugcu gcaggagaac aucacgcugu ucaacacccu caaguccgcc      900

uacgacuucg ucauccugca gagcauccug aagggcaagu ccacccucag cgacgcccag      960

guggagcggu acgacgagca caagaaggac cuggagaucc ugaagaaggu gaucaagaag     1020

uacgacgagg acgggaagcu guucaagcag gucuucaagg aggacaacgg caacggguac     1080

guguccuaca ucggcuacua ccugaacaag aacaagaaga ucaccgcgaa gaagaagauc     1140

agcaacaucg aguucacgaa guacgugaag ggcauccugg agaagcagug cgacugcgag     1200

gacgaggacg ucaaguaccu ccuggggaag aucgagcagg agaacuuccu gcucaagcag     1260

aucuccagca ucaacagcgu gaucccccac cagauccacc uguucgagcu ggacaagauc     1320

cucgagaacc uggccaagua cuaccccucc uucaacaaca agaaggagga guucaccaag     1380

aucgagaaga uccgcaagac cuucaccuuc cggauccccu acuacguggg cccgcugaac     1440

gacuaccaca agaacaacgg gggcaacgcc uggaucuucc gcaacaaggg ggagaagauc     1500

cggcccugga acuucgagaa gaucgucgac cuccacaaga gcgaggagga guucaucaag     1560

cgcaugcuga accagugcac guaccugccc gaggagaccg ugcuccccaa guccagcauc     1620

cuguacuccg aguacauggu gcugaacgag cugaacaacc uccggaucaa cggcaagccg     1680

aucgacaccg acgucaagcu gaagcugauc gaggagcugu ucaagaagaa gaccaaggug     1740

acgcucaaga gcauccgcga cuacaugauc cgcaacaacu ucgccgacaa ggaggacuuc     1800

gacaacuccg agaagaaccu ggagaucguc agcaacauga aguccuacau cgacuucaac     1860

aacauccucg gcgacaaguu cgagcuggag augguggagg accugaucga gaagaucacc     1920

auccacaccg ggaacaagaa gcuccugaag aaguacaucg aggagaccua ccccgaccug     1980

agcuccagcc agauccagaa gaucaucaac cucaaguaca aggacugggg ccggcugucc     2040

cgcaagcugc ucgacgggau cacgggcacc aacaucgaga ccggggagau cgacaccgug     2100

aucaacuucc ugcggaacag cuccgacaac cugaugcaga ucaucggcag ccagaacuac     2160

uccuucaaca acgacaucga caagcuccgc aagaaguaca ucccccagga gaucagcuac     2220

gaggucgugg agaaccugua cguguccccc agcgucaaga agaugaucug gcaggucauc     2280

cggaucacgg aggagaucac caaggugaug ggcuacgacc cggacaagau cuucaucgag     2340

auggccaaga gcgaggaggg gaagaagacc accaucuccc gcaagaacaa gcugcucgcg     2400

cuguacaagg ccaucaagaa ggacgagcgg gacagccagu acgagaagcu gcucacgggc     2460

cugaacaagc uggacgacuc cgaccuccgc agccggaagc uguaccugua cuacacccag     2520

auggggcgcg acauguacac cggcgagaag aucgaccucg acaagcuguu cgacuccacc     2580

cacuacgaca aggaccacau caucccccag agcaugaaga aggacgacuc caucaucaac     2640

aaccuggugc ucgugaacaa gaacgccaac cagacgacca aggggaacau cuaccccgug     2700

cccagcucca uccggaacaa cccgaagauc uacaacuacu ggaaguaccu gauggagaag     2760

gaguucauca gcaaggagaa guacaaccgc cugauccgga acacgccccu caccaacgag     2820

gagcugggcg gcuucaucaa ccgccagcug gucgagaccc ggcaguccac caaggccauc     2880

aaggagcugu ucgagaaguu cuacaagaag uccaagauca uccccgugaa ggcgauccug     2940

gccagcgacc ugcgcaagga caugaacacc cucaagagcc gggaggugaa cgaccugcac     3000

cacgcccacg acgccuuccu gaacaucguc gccggggacg uguggaaccg cgaguucacc     3060

uccaacccca ucaacuacgu gaaggagaac cgggagggcg acaaggucaa guacagccuc     3120

uccaaguacu ucacccgccc gcgcaagagc aaggggaagg ugaucuggac gcccgagaag     3180

ggccggaagc ugaucgugga cacccugaac aagcccagcg uccucaucuc caacgagagc     3240

cacgugaaga agggcgagcu guucaacgcg accaucgccg ggaagaagga cuacaagaag     3300

ggcaagaucu accugccccu caagaaggac gaccgccugc aggacguguc caaguacggg     3360

ggcuacaagg ccaucaacgg ggccuucuuc uuccuggucg agcacaccaa gagcaagaag     3420

cggauccgcu ccaucgagcu guucccgcuc caccuccuca gcaaguucua cgaggacaag     3480

aacacggugc uggacuacgc caucaacgug cugcagcucc aggaccccaa gaucaucauc     3540

gacaagauca acuaccggac cgagaucauc aucgacaacu ucuccuaccu gaucagcacc     3600

aaguccaacg acggcagcau caccgucaag cccaacgagc agauguacug gcgcguggac     3660

gagaucucca accugaagaa gaucgagaac aaguacaaga aggacgcgau ccucacggag     3720

gaggaccgga agaucaugga gagcuacauc gacaagaucu accagcaguu caaggccggc     3780

aaguacaaga accgccggac caccgacacc aucaucgaga aguacgagau caucgaccug     3840

gacacgcugg acaacaagca gcucuaccag cugcuggugg ccuucaucuc ccucagcuac     3900

aagaccucca acaacgccgu cgacuucacc gugaucgggc ugggcaccga gugcgggaag     3960

ccccgcauca cgaaccugcc ggacaacacc uaccucgugu acaagagcau caccggcauc     4020

uacgagaagc ggauccgcau caaguga                                         4047


<210>  1856
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1856
augaaguccg agaagaagua cuacaucggc cuggacgugg ggaccaacag cgucggcugg       60

gccgugaccg acgaguucua caacauccuc cgcgccaagg ggaaggaccu guggggcgug      120

cggcuguucg agaaggcgga cacggccgcc aacacccgca ucuuccggag cgggcgccgg      180

cgcaacgacc ggaagggcau gcgccuccag auccugcggg agaucuucga ggacgagauc      240

aagaaggucg acaaggacuu cuacgaccgc cuggacgagu ccaaguucug ggccgaggac      300

aagaagguga gcggcaagua cucccucuuc aacgacaaga acuucagcga caagcaguac      360

uucgagaagu uccccaccau cuuccaccuc cggaaguacc ugauggagga gcacgggaag      420

guggacaucc gcuacuacuu ccucgccauc aaccagauga ugaagcggcg cggccacuuc      480

cugaucgacg ggcagaucuc ccacgucacc gacgacaagc cgcugaagga gcagcucauc      540

cugcugauca acgaccuccu gaagaucgag cuggaggagg agcucaugga cagcaucuuc      600

gagauccugg cggacgugaa cgagaagcgg acggacaaga agaacaaccu gaaggagcuc      660

aucaagggcc aggacuucaa caagcaggag gggaacaucc ugaacuccau cuucgagagc      720

aucgugaccg gcaaggccaa gaucaagaac aucaucuccg acgaggacau ccuggagaag      780

aucaaggagg acaacaagga ggacuucguc cucaccggcg acagcuacga ggagaaccug      840

caguacuucg aggaggugcu gcaggagaac aucacccucu ucaacacgcu gaaguccacc      900

uacgacuucc ugauccucca gagcauccug aaggggaagu ccacccugag cgacgcccag      960

guggagcgcu acgacgagca caagaaggac cucgagaucc ugaagaaggu caucaagaag     1020

uacgacgagg acggcaagcu guucaagcag gucuucaagg aggacaacgg gaacggcuac     1080

guguccuaca ucggcuacua ccucaacaag aacaagaaga ucaccgccaa gaagaagauc     1140

agcaacaucg aguucacgaa guacgugaag gggauccugg agaagcagug cgacugcgag     1200

gacgaggacg ugaaguaccu gcucggcaag aucgagcagg agaacuuccu gcugaagcag     1260

aucuccagca ucaacagcgu gaucccccac cagauccacc ucuucgagcu ggacaagauc     1320

cuggagaacc ucgcgaagaa cuaccccucc uucaacaaca agaaggagga guucaccaag     1380

aucgagaaga uccggaagac cuucaccuuc cgcauccccu acuacgucgg gccgcugaac     1440

gacuaccaca agaacaacgg cgggaacgcc uggaucuucc ggaacaaggg cgagaagauc     1500

cgccccugga acuucgagaa gaucguggac cugcacaaga gcgaggagga guucaucaag     1560

cggaugcuca accagugcac guaccugccc gaggagaccg ugcugcccaa guccagcauc     1620

cuguacuccg aguacauggu ccucaacgag cugaacaacc ugcgcaucaa cggcaagccg     1680

cucgacaccg acgugaagcu gaagcugauc gaggagcugu ucaagaagaa gaccaaggug     1740

acgcucaaga gcauccgcga cuacaugguc cggaacaacu ucgccgacaa ggaggacuuc     1800

gacaacuccg agaagaaccu ggagaucgcc agcaacauga aguccuacau cgacuucaac     1860

aacauccucg aggacaaguu cgacguggag auggucgagg accugaucga gaagaucacc     1920

auccacaccg ggaacaagaa gcugcucaag aaguacaucg aggagaccua ccccgaccug     1980

agcuccagcc agauccagaa gaucaucaac cugaaguaca aggacugggg ccgccucucc     2040

cggaagcugc uggacgggau caagggcacg aagaaggaga ccgagaagac cgacaccgug     2100

aucaacuucc uccgcaacag cuccgacaac cugaugcaga ucaucgggag ccagaacuac     2160

uccuucaacg aguacaucga caagcugcgg aagaaguaca ucccccagga gaucagcuac     2220

gagguggucg agaaccucua cguguccccc agcgugaaga agaugaucug gcaggucauc     2280

cgcgugacgg aggagaucac caaggugaug ggcuacgacc cggacaagau cuucaucgag     2340

auggccaaga gcgaggagga gaagaagacc accaucuccc ggaagaacaa gcugcuggac     2400

cucuacaagg cgaucaagaa ggacgagcgc gacagccagu acgagaagcu gcugacgggc     2460

cucaacaagc uggacgacuc cgaccugcgg agccgcaagc ucuaccugua cuacacccag     2520

auggggcggg acauguacac cggcgagaag aucgaccugg acaagcuguu cgacuccacc     2580

cacuacgaca aggaccacau caucccccag agcaugaaga aggacgacuc caucaucaac     2640

aaccuggucc uggugaacaa gaacgccaac cagacgacca aggggaacau cuaccccgug     2700

cccagcucca uccgcaacaa cccgaagauc uacaacuacu ggaaguaccu cauggagaag     2760

gaguucauca gcaaggagaa guacaaccgg cugauccgca acacgccccu gaccaacgag     2820

gagcucggcg gguucaucaa ccggcagcug gucgagaccc gccaguccac caaggccauc     2880

aaggagcugu ucgagaaguu cuaccagaag uccaagauca uccccgugaa ggccagccuc     2940

gccagcgacc ugcggaagga caugaacacc cugaaguccc gcgaggugaa cgaccuccac     3000

cacgcgcacg acgccuuccu gaacaucguc gccggcgacg uguggaaccg ggaguucacc     3060

agcaacccca ucaacuacgu gaaggagaac cgcgagggcg acaaggucaa guacucccug     3120

agcaaggacu ucacccgccc gcgcaagagc aaggggaagg ugaucuggac gcccgagaag     3180

ggccggaagc ucaucgugga cacccugaac aagcccuccg uccugaucag caacgagucc     3240

cacgugaaga agggggagcu guucaacgcc accaucgccg gcaagaagga cuacaagaag     3300

ggcaagaucu accugccccu gaagaaggac gaccggcucc aggacgugag caaguacggg     3360

ggcuacaagg cgaucaacgg ggccuucuuc uuccuggucg agcacaccaa guccaagaag     3420

cgcauccgga gcaucgagcu guucccgcuc caccuccugu ccaaguucua cgaggacaag     3480

aacacggugc ucgacuacgc caucaacgug cugcagcugc aggaccccaa gaucaucauc     3540

gacaagauca acuaccgcac cgagaucauc aucgacaacu ucagcuaccu caucuccacc     3600

aagagcaacg acggcuccau caccgucaag cccaacgagc agauguacug gcggguggac     3660

gagaucagca accugaagaa gaucgagaac aaguacaaga aggacgccau ccugacggag     3720

gaggaccgca agaucaugga guccuacauc gacaagaucu accagcaguu caaggccggg     3780

aaguacaaga accggcgcac caccgacacc aucaucgaga aguacgagau caucgaccuc     3840

gacacgcugg acaacaagca gcuguaccag cuccuggugg cguucaucag ccuguccuac     3900

aagaccagca acaacgccgu cgacuucacc gugaucggcc ucggcaccga gugcgggaag     3960

ccccggauca cgaaccugcc ggacaacacc uaccuggugu acaaguccau caccggcauc     4020

uacgagaagc gcauccggau caaguga                                         4047


<210>  1857
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1857
auggagaaga acuacuaccu gggccucgac cuggggagca acuccguggg cugggccgcc       60

accgacgagg acuacaacuu ccugcgccuc aaggggaaga ccgcgugggg cgcccggauc      120

uucagcgagg ccaaggacug caaggagcgc cggacguacc gcuccaacaa gcggcgccug      180

gcccggcgca aguaccggcu gguccuccug aagcagcugu ucgccgagga gaugaccaag      240

guggacaaga ccuucuuccu ccgccuggcg aacagcaccu acugggagga ggacaagacg      300

aaggacuccg acgggaacga gaucggcaag aaccugaucu ucaagaccca cgaggaggag      360

aagcaguacu acaccaagua ccccaccauc uggcaccucc ggaaggcccu gaucgagaac      420

gacgaggagg cccugagcaa ccuccgcuac guguaccugg ccauccacca caucgucaag      480

uaccggggca acuuccugac ggagggggag augggcaacg acaucccgcc caucaccacc      540

aucgaggaga ucaaccagac ccucucccug cuggaggugg acagcuccga ggacgcgacg      600

gacaagacca ccuacaucaa gcccgagagc uacaacgagc uguucaccau ccucgagaac      660

aacaaccuga acaaggugaa gaagcagcag gagaucgaca agcuguucau cuccagcaag      720

gagcgcuccg agaacaucaa gaacuacauc aaccucuuca agacgaucau cgucgggggc      780

agcuucuccc ugaagaagau caacgaggac accgaggaca ucagcgugga cuucaccaag      840

gacuucgaga acuccgaggc cgacaucagc aaggcccucg gggacuccuu caaccuggug      900

cugaucgcca agaaguacua cgacuacauc auccucaagg agcugcuggg caagaacaag      960

gagaucagcg aggccauggu ccagaucuac gaggagcaca aggacgaccu caagagccug     1020

aagaccaucc ugaaggaccu cgacaacaag cugggcaacg acgggaagga ccggaucuac     1080

uacuccgugu ucaagcagga gcccacggag aaguucaagg agaccaacua ccugagcuuc     1140

gugcagggcg ggaagcucga ggagcugaac aaguacauca agaagauccu ggagaacaac     1200

gagaaggaca ucgacgacaa ggagaccuac aacgcgcucu acgagaaggc cugcaagggc     1260

gaccuguuca agaagcaggc caacgccugc accucccuga ucccgcacca guuccaccuc     1320

cgcgagcuga agaagauccu ggagaacgcc ggccggaagu acagcuuccu caacgacauc     1380

uccgacaaga ucaucaagau cuucaaguuc cgcguccccu acuacuacgg gccccugaac     1440

aacgagagca acgcgcacuc cuggguggug aagaaggagg gccaggagaa ggccaagauc     1500

acgcccugga acaucaacga gaucaucgac accccgaaga cccgggacaa cuucuucaag     1560

aagcugacca acagcugcac guaccugguc ggggagaccg ugcuccccaa gaccucccug     1620

uacuacgaga aguaccuggu gcucgaccgc cugaacaaga ugcaggucaa cggcgacuac     1680

cugagcgccu ccgagaagaa cgacgugcuc aaguucaucc ugagccggaa gaccacgacc     1740

aucgagcaga uccgcaccua ccuguccaag aacaagagca ccaaggccaa cgacaucucc     1800

aucagcaaca ucaaggccac ggaccccuuc aacgcggugu cccacgccuu cuacgggaag     1860

caccccgacc ucaacaucaa ccugugcgag gagcugauca aguucgccac caucuacgcc     1920

gacgacaaga aggagcucaa gaaguucguc agcuccaacu ucaaccugac cgacgaguuc     1980

aucaaggagu accugaacac cccgaccaac aaguggggcc gccucagccg gaaguuccug     2040

ugcggcgggg accagcugga gauguacuac guggacgaca acggcauccu ccacagcauc     2100

cugaacaucc uggaggagac gaacgagaac cuccaggaga uccugaacaa cgagaaguac     2160

ggguucaacc agaagaucgc cgacuucaac aacacccuga ccgcggacaa gaccacggag     2220

gagcagaucg aggacauccu cgacuccacc cccgcccuga ugcgccggag caucauccag     2280

acccuggccg uggucgacga cgugaucaag gccgcgggca aggcgcccca gaagaucaug     2340

auggagguca cccgcacgga cgggaagaag gaggagacca agucccggua cgugcagcuc     2400

aagaagcaga ucaccaacga caaggugcug accagcgacg agaagaagga gcugcucacg     2460

aaccuggaga acaagaagau ccagcugaag ggcaagcaca ucuaccucua cuucaugcag     2520

augggcaucg acuucuacac cgggaagauc auggacauca acgacgucau caacggcaag     2580

uacgacaucg accacaucau cccccagucc augaucaagg acgacagccu ggacaacaug     2640

gugcuggugu ccaaggacua caaccagaag gucaagaagg acaucuaccc gaucugcgac     2700

gagauccgca acaacaugcu cgacaagugg aagggguacu acgcccggaa gaucaucagc     2760

gaggagaagu acaaccgccu gaagcggacc aaggagcuga acgccgacga gaucaacgcc     2820

uucaucaacc gccagaucaa cguggugaac uacuccaaca uccagaucaa gcggaucuuc     2880

gagaucaagu accccgagac caagaucauc uucagcaagg ccuccuuccc cagccuccug     2940

cgcaaggagu acgagaucgc caagaugcgg gaccugaacg acgcccacca cgccgucgac     3000

gccuaccuca acaucgugug cggcaacauc cugacgaccc uguacaccga caucuucuac     3060

auguucaagg agaagaagga ggaccacaag aacaucuccu ucaacaugga gaagcgccuc     3120

aucaacuaca ucgacaagaa caaccugaag gacaagauca agaccagcug ccugcggcac     3180

gacgcgcuca ucacguacaa gcccgacuac gacuccggga aguucuacaa ggagaccauc     3240

uucaagaagu ccggcgacaa ggagaccggc cugaucccgc ugcacaccaa ggggcccaug     3300

agcgacacga agaaguacgg cggguacagc cagcucagcc aguccuacau caucgccgug     3360

gaguacaagc ugaagaacaa gaccgucaag aagcugugcc gcgugcccac ccucuaccug     3420

aagcaguaca agaacgaccu gaacaccauc gccaagauca ugaucgacga cgagaaggcc     3480

acggacauca aggugcuccg gaagaucuac cugaaccaga agauccgcua caacggcugc     3540

uacuaccuga ucuacaccag caaggagaag cagaacaagu acaagauggc cuaccagaac     3600

uaccucgaca acgacuaccu gaucuaccug aacaagugcc ucaagaagau cgacgagcug     3660

gacgacggcg agcugaccuc ccaggaguuc aucgucaaca aggacgggga caaguuccuc     3720

aucgacaaga accacaaccu ggagaucuuc aagagcauca aggagcagua caacaagacc     3780

aucuacgacu acacgcccua caucgugcag ucccgggaga ugaaggagga ggacuucaac     3840

aaccugagcc ucaagaagca gaucgacacc cugcugaaca ugaucaagau ccucucccgc     3900

agcaacgagc aggcgaaguu cgagaccgcc uucuucgaga acuucaccaa gguguuccug     3960

aagacgauga ccaucaccaa cgagaagguc uacaucguga acgagucccc gagcggccug     4020

uucaagggga aggaggagcu caucuga                                         4047


<210>  1858
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1858
augaagaagu ucgaggacua cuaccugggc cucgaccugg ggaccagcuc caucggcugg       60

gccgugacca acgagaagua cgacauccug cgcuucaaca agaaguucau gugggggacg      120

caccucuucg acaugggcaa caccgccagc gagcggcgca ucuuccggac cucccgccgg      180

cgccugaagc ggaagaagaa ccgcaucgag cugcuccaga cccuguucag cgcggagaug      240

uccaagaagg acuucggguu cuuccagcgg cugaaggaga gccaguacuu caucgaggac      300

aagacguuca agaacaagaa cacccucuuc auggacgaga acuacaccga ccaggacuac      360

cacauggagu accccaccau cuaccaccuc cgcaaggccc ugaucgacaa cgaggagucc      420

gccuucgaga uccggaagcu guaccuggcc aucagccaca uccuguccca ccgcggccac      480

uuccucuucg agggcgagcu gagcgacaac cuggacuucg acgacaucuu caacgaguuc      540

gucaaguacc ucaacgagga gcugaacauc gacuucaaga ucaaguccga cgugagccug      600

caggccauca aggagauccu cacggacaag augcugaacc ggaccaagaa gaaggagaag      660

cuguccgagc uccuggagga ggagaagaac aagaucuacc aggagaucug gaagcugcuc      720

guggggaacg aggcgaagau gagcaccauc uucgacgaca aggaguacga ggaggccaag      780

aucgccuucu ccaagggcaa cuacgacgag aaggaggagg aguacgagag cauccugggg      840

gagaagaucc uguucgucca gaaggccaag gcgaucuacg acuggaugcu ccugcagaac      900

auccugaagg aggacaagua ccucuccuac agcaagguga agcuguacaa cgagcacaag      960

aaggaccuga agauccucaa ggacgugcug aagaaccugg acaagaagau cuacaaggag     1020

aucuucuccg agaacggccu cuacgaggag uaccugcgca ucgagaagaa gaagaucggg     1080

agcaaggagg agaagaccaa ggacauccug uacaaccggc ucaagaagau cuucgagaac     1140

cgcgacaaca uggccaucga ggacauccug aacaagaucg agcuggacag cuuccucccg     1200

aagcagaaga ucaaggacaa cggcgucauc ccccgccagg ugcaccagaa ggagcuggag     1260

aucauccuga agaacgccga gaaguacuuc aaguggcuca ucgagaagga cgagacgggc     1320

cuguccguga gcgagaagau caugaagauc uucaaguuca agauccccua cuacgucggg     1380

ccccugaacu ccaagagccc guucgccugg aucgagcgga aggaguacgg caagauccug     1440

cccuggaacu ucgagaacau gguggacaug uccaagaccg ccgagaaguu caucaagcgg     1500

augaccaaca agugcaccua ccuccccgag aaggacguga uccccaagaa cagccugcug     1560

uacacgaagu acaugguccu caacgagcug aacaaccuga agauccgcgg ggagaagauc     1620

aagguggagc ucaagcagaa gaucuacacc gagcuguucc agaagaacaa gaagaucggc     1680

augaagaccc ugaucgaguu ccucaagucc cagaacaucg uggucaccaa gguggagauc     1740

acgggcaucg acgggaccuu caacaccagc cugggcuccu acaucgacau gaagaagauc     1800

uucggggagg agaagaucgu gaaggacagc uacaagaaga ugaucgagga caucauccug     1860

uggaucaccc ucuacggcga ggagaagaag cugaucaagu ccaagaucga ggagaaguac     1920

ccggagaagg ucacgcccga ggagaugaag cugauccuca agcugaagua ccgggacugg     1980

agccgccugu ccaaggaguu ccucaccgag aucgagacca uggacgagga gaccggggag     2040

cugaucagca ucaucgagau gcuguggcgc aagaacgaga acaucaugga gcuccugucc     2100

uacaaguacg acuucagcaa ccggaucaag gaguacaacg gcaucaacga gaaggagaag     2160

aacaucacgu acgaggaccu ggugaaggac cuccagaucu cccccagcgu gaagaagaug     2220

aucuggcgcg cgcuggucau cgugaaggag aucaagaagg ugaccaacaa gucccccaag     2280

aaggucuuca ucgagauggc ccgggacacc cagaccgaga agaagcgcac ggagaagcgg     2340

aaggacaagc ugcucgagcu guacaagaac aucaagaacg acgacagcau caaggagcug     2400

gagaagaacc ucaaggacaa gaccaacgac gaccucaaga gcaagaagcu guaccuguac     2460

uaccugcagc ugggccgcug cauguacucc ggggagguga uccggcucga ggaccugaug     2520

aacgacaacc uguacgacau cgaccacauc uacccgcaga gcaagaucaa ggacgacucc     2580

uucgacaaca ccguccucgu gaagaagcag aucaacgccg acaagaccaa cgaguacccc     2640

aucgacaaga gcauccagaa caagugcaag gccuucugga cgucccugaa ggagcgcggc     2700

cugaugagca acaccaagua cgagcggcuc acccgcaccg agggguucuc cgacgacgag     2760

cuggccggcu ucaucagccg gcagaucgug gagacccgcc aggcgacgaa gguccuggcc     2820

gaccuccuga aggugaucuu cggguccgac acccggaucg uguacgucaa ggccggccug     2880

accagcgacu uccgccagaa guucggcauc uacaaguucc gggagcucaa cgacuaccac     2940

cacgcccacg acggguaccu gaacaucgug accggcaaca ucuacaacac gcuguucacc     3000

gacaaccccu acaacuucau caaggaccgc aagaaggaca accggaccua caaccucaac     3060

aagguguucg agcgcgagaa cgacgucugg aaccccgacg ugaugcuguc caagaucgag     3120

cgguacaucu acaagauccg cccgcuguuc acccgcgcca gcuacgagca gaagggcggg     3180

uucuccgacc agaacaucag cuccgagaag gacaagcucc ggccccucaa gagcuccggg     3240

gugcugagca acaucaagua cggcggcuac gacaagauca agaacagcua cuucuucauc     3300

gucgaguaca cggagaagaa gaagcgcgug cgguccaucg agacccugcc caucuacauc     3360

aagaagucca ucagcaccaa ggaggaccug gagaaguacu gcaaggagga gcugaagcuc     3420

caggagccca aggugcuggu cagcaagcug aacuacaagu cccucaucua cagcaacggg     3480

cacgaguacu acaucaacuc caagaccgcg gacagcauca cgauccagaa cggcauccag     3540

aucauccuga ccaaggagaa cuacgagacc uaccgcaaga ucuacaagaa ccuggaguac     3600

cucaagcuga ucuacaagga cgagaagauc cgggaggaga agauccugga gaagaucccg     3660

aagauccuca agaacaacaa gaacgaccgc gaccuguccg aggacgagau cagcgagaag     3720

uucuggaagc uguacaagga gaucuucgag aagaucaaga ucgggaucuu cuccaaguuc     3780

aagagcaccc uccccaucua caucgaggac ugcaagaagc ugaaggacuu cacgacccug     3840

aacaacuacg ccaagucccg guucauccag gagauccuca agugccugaa cagccagcag     3900

ggcgaguucg acaccaagua cacccacaac uacuccaaga aguacaacac gaagcugagc     3960

aaguccaaga uccucgagaa cgacuucuac aucaucaacc agagccccac cggccuguuc     4020

gagaagaagg uguuccugga caaguga                                         4047


<210>  1859
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1859
augggggagg acgggaagcc ccugcgcgug aagggcaaga ccgccaucgg ggcccggcug       60

uuccaggagg gcaacccggc ggccgaccgc cggauguucc gcaccacgcg gcgccggcug      120

aaccgccgga aguggcgccu gaagcuccug gacgagaucu ucgaccccua caucaccccc      180

gucgacagca ccuucuucgc ccggcugaag caguccaacc ucagccccaa ggacucccgc      240

aaggaguuca aggggagcau gcuguucccg gaccugaccg acaagcagua ccaccucgac      300

uaccccacga ucuaccaccu ccggcacgcc cugaugaccc aggacaagca guucgacauc      360

cgcauggugu accucgccau ccaccacauc gugaaguacc ggggcaacuu ccugaacucc      420

acccccgucg acagcuucaa ggcguccaag gugaacuucg acgaccaguu caagaagcug      480

aacgagcucu acaccgccau caaccccgag gagagcuucc agaucaaccu ggccaacucc      540

gagaagaucg gccagcaguu ccuggacccg agcauccgca aguucgacaa gaagaagcag      600

auccccaaga ucgugcccgu cacgauggac gacaaggccg uggacaagcu caacgggaag      660

aucgcguccg agaucaucaa cgccauccug ggcuacaaga gcaagcugga cguggccguc      720

cagugcgacc ccgaggacuc caacccgugg gccgugaagu ucgacgacga ggacaucgac      780

gccaagcucc agaagauccu gcccgagaug gacgagaacc agcagagcau caucgggauc      840

auccaggacc uguacuccca ggugacccuc aaccagaucg uccccaacgg caugagccug      900

uccgagagca ugaucaagaa guacaacgac caccacgacc accugaagcu cuacaagaag      960

cugaucgggc agaucuccga cagcaagaag aagaccgcgc ugaagaaggc cuacgaccag     1020

uacgugggca acgacggcaa ggugaucgag caggacgacu ucuacagcuc cgucaagaag     1080

aaccucgacg acagcgagcu guccaagcag aucauggacc ugaucgacgc cgacaaguuc     1140

augcccaagc agcggaccag ccagaacggg gugaucccgc accagcucca ccagcgcgag     1200

cuggacgaga ucaucgagca ccaguccaag uacuaccccu ggcuggccga gagcaacccc     1260

aacaagcacg accuccaccu ggccaaguac aagaucgagg agcugguggc guuccggauc     1320

cccuacuacg ucggcccgau gaucacgccc gaggaccaga agaaguccgg ggccgccgug     1380

uucagcugga ugaagcgcaa ggagcccggc cagaucaccc ccuggaacuu cgacgagaag     1440

guggaccgga uggaguccgc caacaaguuc aucaagcgca ugaccaccaa ggacacguac     1500

cucaucggcg aggacguccu gccggacgag agccugcucu acgagaaguu caaggugcug     1560

aacgagcuga acauggugcg cgucaacggg gacucccuca agguggccga caagcaggcg     1620

aucuaccagg accuguucga gaacuacaag cacaucagcg ucaagaagcu gcagaacuac     1680

aucaagggca agaccgggcu ccccaacaac cccgagaucu ccggccugag cgaccccgac     1740

caguucaaca acucccuggg gaccuacaac gacuucaaga agcuguucgg cgacaaggug     1800

gacgacccgg accuccagaa cgacuucgag aagaucgugg aguggagcac cgucuucgag     1860

gacaagaaga uccugcgggc caagcugaac gagaucacgu ggcucaccga caagcagaag     1920

gacgaccugg aguccagccg cuaccagggc ugggggcggc uguccaagaa gcuccugacc     1980

ggcaucguga acgaccaggg ggagcgcauc aucgacgagc uguggaacac caacaagaac     2040

uucaugcaga uccagagcga cgacgacuuc gccaagcgga uccacgaggc caacgccgac     2100

cagaugaagu ccacgaacau ggaggacgug cucgcggacg ccuacaccag cccccagaac     2160

aagaaggcca uccgccaggu cgugaaggug gucgacgaca uccagaaggc caugggcggg     2220

guggcgccca aguucaucag caucgaguuc acccgguccg aggaccgcaa cccgcggcgc     2280

accaucagcc ggcagcgcca gcuggagaac acgcugaagg acaccgccaa gucccucguc     2340

aagagcauga accccaacau ccuguccgag cuggacaacg ccagccgguc caagaagggc     2400

cucaccgacc gccuguaccu guacuucacc cagcucggca aggacaugua cacgggggag     2460

cccaucaaca ucgacgagau cagcaacuac gacaucgacc acauccugcc ccaggccuuc     2520

aucaaggacg acucccugag caaccgggug cucgugucca aggccgugaa caacggcaag     2580

agcgcgaacg ucccgcugaa gcuguucggg gccaagaugg gccacuucug gaagcagcuc     2640

gccgaggccg ggcugaucaa caagcacaag cugaagaacc uccagaccaa ccccgacacc     2700

aucuccaagu ucgccaugca cggcuucauc cgccggcagc ugguggagac cagccaggug     2760

aucaagcugg ucgcgaacau ccucggcgac aaguaccgca acgacgacac gaagaucauc     2820

gagaucaccg cccggaugaa ccaccagaug cgcgacgagu ucggguucau caagaaccgc     2880

gagaucaacg acuaccacca cgccuucgac gccuaccuga ccgccuuccu gggccgguac     2940

cucuaccacc gcuacaucaa gcugcggccc uacuucgugu acggggacuu caagaaguuc     3000

cgcgaggaca aggugaccau gcggaacuuc aacuuccugc acgaccucac cgacgacacg     3060

caggcgcgca ucgccgacac cgagaccggc gaggucaucu gggaccggga gaccuccauc     3120

aagcagcugc gcgacgugua ccacuacaag uucaugcuga ucagccagga gguguacacg     3180

cuccggggcg ccauguucaa ccagaccauc uaccccgcca gccaggcggg gcgcaagaag     3240

cugaucccga ugaaggccga caagcccguc gacguguacg gcggguacuc cggcagcacc     3300

gacgccuaca uggccaucgu gcggauccac gacaagaagg gggacaagua caaggucgug     3360

ggcgugccca ugcgcgcccu gaaccggcuc aacaccgcga agaacgucuc cgacgccaag     3420

uacgaccagg agcugaagga ggugcugacg ccccaguuca ccaagagcaa gaagaaccgc     3480

aagaccggcg agaucaccca gacgguggag gacuucgagg ucgugcucgg gaaggugaug     3540

uaccggcagc uggucaucga cggcgacaag aaguucaugc ugggguccag caccuaccag     3600

uacaacgcca agcagcucgu gcuguccgac gagagcauca agacccuggc cuccgagggc     3660

aagcucaacg aggcccagga gagcgcggcc uacgacaagg uguacgacga gauccuggac     3720

aaggucaaca aguacuucgc ccuguacgac accaacaagu uccgccagaa gcucucccug     3780

gggaaggaca aguucguggg ccugccgaac cacaacgugu ucgacggcag caagaagauc     3840

uccagcggga agcgggagau ccucaacgag auccugaacg gccugcacgc caacgccacg     3900

cucgggaccc uguccgacau cggcuucagc acccccuucg ggaagaugca gcagccccgc     3960

ggcaucggcc uguccagcaa caccuccauc gaguacgaga gcuucacggg ccucuucaag     4020

cggaccaucu uccugaagga ccuguga                                         4047


<210>  1860
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1860
augaagaagc ccuacaccau cgcccuggac aucggcacca acagcguggg gugggucgug       60

gugacggacg acuaccgcgu cccgaccaag aagaugaagg ugcucggcaa caccgagcgg      120

aagaccauca agaagaaccu gaucggggcc cugcuguucg acuccggcga cacggcggag      180

gggacccgcc ugaagcggac cgcccgcccc cgguacaccc gccggaagaa ccgccugcgg      240

uuccucaagg agaucuucac ggaggagaug gccaaggugg acgacggcuu cuuccagcgc      300

cuggaggaca gcuucuacgu ccuggaggac aaggagggca acaagcaccc caucuucgcc      360

aaccucgccg acgagguggc guaccacaag aaguacccca ccaucuacca ccuccggaag      420

gagcuggugg acaacccgca gaaggccgac cuccgccuga ucuaccuggc cgucgcccac      480

aucaucaagu uccgggggca cuuccucauc gagggcaccc uguccagcaa gaacaacaac      540

cugcagaagu ccuucgacca ccucguggac accuacaacc ugcuguucga ggagcagcgc      600

cuccugacgg aggggaucaa cgcgaaggag cugcucagcg ccgcccuguc caagagcaag      660

cggcuggaga accucaucuc ccugaucccc ggccagaaga agaccgggau cuucggcaac      720

aucaucgccc ugagccucgg ccugaccccc aacuucaagg ccaacuucgg gcuguccaag      780

gacgugaagc uccagcuggc gaaggacacc uacgccgacg accuggacag ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugac ggaguccgac gagaucaccc gcgcgccccu gagcgccucc      960

auggucaagc gguaccgcga gcaccacaag gaccucguga cccugaagac ccugaucaag     1020

gaccagcucc cggagaagua ccaggagauc uuccuggaca agacgaagaa cggguacgcc     1080

ggcuacaucg agggccaggu gagccaggag gaguucuaca aguaccugaa gcccauccug     1140

gcccggcucg acggguccga gccccugcug cucaagaucg accgcgagga cuuccugcgg     1200

aagcagcgca ccuucgacaa cggcagcauc ccccaccaga uccaccugga ggagcuccac     1260

gccauccugc gccggcagga ggucuucuac ccguuccuga aggacaaccg caagaagauc     1320

gagagccucc ugaccuuccg gauccccuac uacguggggc cccuggcccg cggccacucc     1380

cgguucgccu gggugaagcg caaguucgac ggggccaucc ggcccuggaa cuucgaggag     1440

aucgucgacg aggaggcgag cgcccagauc uucaucgaga agaugaccaa gaacgaccuc     1500

uaccugccga acgagaaggu gcugcccaag cacucccucc uguacgagac guucaccgug     1560

uacaacgagc ugaccaaggu caaguacgcc accgagggca ugacgcgccc ccaguuccuc     1620

agcgccgacc agaagcaggc gaucguggac cugcuguuca agaccaaccg gaaggugacc     1680

gucaagcagc ucaaggagaa cuacuucaag aagaucgagu gcugggacuc cguggagauc     1740

accggcgucg aggacagcuu caacgccucc cuggggacgu accacgaccu gcucaagauc     1800

auccaggaca aggacuuccu ggacaacccc gacaaccaga agaucaucga ggacaucauc     1860

cugacccuca cccuguucga ggacaagaag augaucagca agcgccugga ccaguacgcc     1920

caccucuucg acaagguggu gcugaacaag cuggagcggc accacuacac cggcuggggg     1980

cgccucuccg gcaagcugau caacgggauc cgggacaagc agagcggcaa gacgauccug     2040

gacuuccuca aggccgacgg cuucgccaac cgcaacuuca ugcagcugau ccacgacucc     2100

gagcugagcu ucaucgacga gaucgcgaag gcccagguca ucgggaagac cgaguacucc     2160

aaggaccucg ugggcaaccu ggccgggagc ccggccauca agaagggcau cucccagacc     2220

aucaagaucg uggacgagcu ggucaagauc augggguacc ucccccagca gaucgugauc     2280

gagauggccc gggagaacca gaccaccgcg cagggcauca agaacgcccg ccagcggaug     2340

cgcaagcugg aggagacggc caagaagcug ggcagcaaca uccucaagga gcaccccgug     2400

gacaacuccc agcugcagaa cgacaagcgg uaccuguacu accuccagaa cgggaaggac     2460

auguacaccg gcgacgaccu ggacaucgac uaccugagcu ccuacgacau cgaccacauc     2520

aucccccaga gcuucaucaa gaacaacagc aucgacaaca agguccucac cucccagggg     2580

gccaaccgcg gcaagcugga caacgugccg agcgaggcca ucgugcgcaa gaugaagggc     2640

uacuggcagu cccugcuccg ggcgggggcc aucagcaagc agaaguucga caaccugacc     2700

aaggccgagc gcggcgggcu gacgcagguc gacaaggccg gcuucaucca gcuccagcug     2760

guggagaccc ggcagaucac caagcacgug gcgcagaucc uggacucccg cuucaacacc     2820

gaguucgacg accacaacaa gcggauccgc aagguccaca ucaucacgcu caagagcaag     2880

cugguguccg acuuccggaa ggaguucggg cuguacaaga uccgcgacau caaccacuac     2940

caccacgccc acgacgccua ccucaacgcc guggucgcca aggcgauccu gggcaaguac     3000

ccccagcugg cccccgaguu cguguacggc gacuacccca aguacaacag cuucaaggag     3060

cggcagaagg ccacccagaa gacccucuuc uacuccaaca uccugaaguu cuucaaggac     3120

caggagagcc ugcacgugaa cuccgacggg gaggagaucu ggaacgccaa caagcaccuc     3180

ccgaucauca agaacguccu gagcaucccc caggugaaca ucgugaagaa gaccgagguc     3240

cagacgggcg gguucuacaa ggaguccauc cugagcaagg gcaacuccga caagcucauc     3300

ccccgcaaga acaacuggga cacccggaag uacgggggcu ucgacagccc gaccguggcc     3360

uacuccgugc uggucaucgc gaagauggag aagggcaagg ccaaggugcu gaagcccgug     3420

aaggagaugg ucgggaucac caucauggag cgcaucgccu ucgaggagaa ccccguggug     3480

uuccucgagg ccaagggcua ccgggagauc caggagcacc ugaucaucaa gcugcccaag     3540

uacagccucu ucgagcugga gaacgggcgc cggcgccugc ucgccagcgc guccgagcug     3600

cagaagggca acgagcuguu ccucccgguc gacuacauga cguuccugua ccuggccgcc     3660

cacuaccacg agcucaccgg gagcuccgag gacgugcugc ggaagaagua cuucguggag     3720

cgccaccucc acuacuucga cgacaucauc cagaugauca acgacuucgc cgagcggcac     3780

auccuggcga gcuccaaccu ggagaagauc aaccacaccu accacaacaa cagcgaccug     3840

cccgucaacg agcgcgccga gaacaucauc aacguguuca ccuucguggc ccucggcgcc     3900

cccgccgcgu ucaaguucuu cgacgccacg aucgaccgga agcgcuacac cuccaccaag     3960

gagguccuga acgccacccu gauccaccag agcgugacgg gccuguacga gacccgcauc     4020

gaccucuccc agcuggggga gaacuga                                         4047


<210>  1861
<211>  4050
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1861
augaagcaga aguucuacgu gggccuggac auggggaccg ccagcguggg cugggccgug       60

accgacgaca aguacaacau caucaagcgc cacgggaagg cgcucugggg cgugcggcug      120

uucaagacgg ccaacaccgc cgaggagcgc cggaucuucc gcgccgcccg gcgccggacc      180

cagcgccgga agcagcgcau ggagcugcuc caggagaucu ucggggacga gaucuccaag      240

accgacaucg gcuucuacca gcggaugaag gagagcaagu acugguucga ggacaagcgc      300

gacaucaacg gcaagcagcc cgagcugccg uacgcgcugu ucgucgacga cggguucacg      360

gacaaggacu accacgagca guuccccacc aucuuccacc uccggaacgu gcuggugaac      420

gaguggaccc agaaguacga cauccgccug gucuaccucg ccauccacca caucaucaag      480

caccggggcc acuuccuguu cgaggggaag aagauguccg aggugaccga cuucggcagc      540

gccuucgagg ccuucugcaa gguggcgucc gacaacgaca ucgagaucac ggucgacgag      600

gagaagcuga aggacaucga ggaccuccuc aagagccgcc agaaguccaa gaccgccaag      660

aagcaggagc uggggaagau caucgccggc gccgacaagc agaagaaggc ccucgcggcc      720

cuggugaccg gcugcauggu gaagcugagc gagaucuucg ccgacaaguc ccucgacgac      780

ggggagcgga acaagaucag cuucgccgac uccaacuacg acgaguacau cgccaacguc      840

gaggagaccc uggcggagcg cuuccugcuc aucgccgccg gcaaggcccu guacgacugg      900

gccgugcuga gcgacauccu cgaggggucc acguacauca gcgaggcgaa ggugaagauc      960

uacaagaagc acggcgacga ccugcggaag cugaagggcc uccugaagac ccgccccgac     1020

gaguacaagc ugaucuucgg gcuccccgag aagcaggcca acuacuccgc cuacaucggc     1080

auggucaaga agaacgggaa gaaggugccg aucgacaaga agugcaccaa ggccgacuuc     1140

uacagcuucc ugaagaagac ccugaacaag aucaacuucg aggacgagaa ggagcgggag     1200

aucgccgagu ccaucaucaa cgagaucgag aacgagacgu uccuccccaa gcaggucauc     1260

agcgacaacg gcgugauccc cuaccagcug cacgaggcgg agcuggaccg cauccucaag     1320

aacgccgccc uguacuaccc cuuccugucc gagaaggacg acgaggggua caccaacaug     1380

gacaagauca aggccaucuu caaguuccgc aucccguacu acgugggccc ccucaacacc     1440

uaccacagcg acaagggcgg gaacagcugg gcggugcgga agcaggacgg caagaucuac     1500

cccuggaacu ucgaguccaa gaucgacgcc ggggagagcg cccgcaaguu caucggccgg     1560

augaccaaca agugcacgua ccugaucggg aaggacgucc ugcccaagga gucccuccug     1620

uacagcaagu ucauggugcu gaacgagcuc aacaaccugc gcaucgacgg cgagaaggug     1680

uccgucgaga ucaagcaggc cguguacgag aacgucuuca agaaguacca gcgggugacc     1740

ggcaagaagg ugaaggacuu ccugauccgc agcgggauca ucggccggga gcaggagcuc     1800

uccggguucg accagaacuu caagagcucc cugaaggccu accacgacau caagcagauc     1860

gcgggcgaca ucaaccugag ccagaaccag aaggaggaca ucaucaagga gaucaccguc     1920

uucgccgacu cccagaagau gcugaagaag cgccucaaga ucaaguaccc ggagcugagc     1980

gacaagcagg ucaccaagcu ggccgccaag aaguacacgg gguggggccg gcucucccgc     2040

gaguuccugg aggaggugga gagcgugaac aaggagaccg gcgaggugcu gaacaucauc     2100

uccgcccucu gggagaccaa cgagaaccug augcagcugc ucagcaacaa guacgacuac     2160

cuguccgaga ucaagcggcu gaacgacgag gcggaggaca agagcgaggu ggucaccuac     2220

gacgacgugc agaagcucua cguguccccc gccgucaagc gccccgugug gcagacgcug     2280

aagaucgugg aggagaucag caagaucaug gggagcaagc ccgagcggau cuucgucgag     2340

auggcccgcg agccgggcca gaagggggac gugaaggugu cccgcaagag ccggcugcuc     2400

gagaaguaca aguacugcaa gaaggaggcc cccgagaucu acgagaaccu ggucaaggag     2460

gacgacaaca agcugcgguc cgacaagcug uuccuguacu acacccagau gggccgcugc     2520

auguacagca acgaggugau cgacaucgag gaccuguuca ccaaccggua cgacaucgac     2580

cacaucuacc cccaguccaa ggugauggac gacagccucg acaaccgcgu ccuggugaag     2640

cgcgagcuga acuccaacaa gaccgacgug uuccccgucc cgaaggagua cauggacaag     2700

gccaagacgc ucugggacau gcugaagaag aaggaccuga ucagcaagga gaaguaccac     2760

cggcucaccc gccgggacgg cuucuccgag aacgagcuga gcggguucau cgcgcgccag     2820

cugguggaga cccggcaguc caccaaggcc gugacgcagc uccugaccaa ggccuacccc     2880

gagagcgaga ucgucuacgc caaggcgaac gccguguccg aguuccgcca caaguucggc     2940

uucaccaagg ugcggagcau caacgacuac caccacgcca aggacgccua ccugaacauc     3000

gucgugggga acaccuacua cgugaaguuc accaaggacg ccgcgugguu cauccgcaac     3060

aaccccggcc ggacguacaa ccucaagaag auguucgagc aggggaccgu cucccgcggc     3120

ggcgagaccg ccugggugag cggggaggac ggcaccaucu ucacggugaa gcgggucaug     3180

gggaagaaca acauccuguu cacccgcaag gccuacgagg ugcggggcgg gcuguucgac     3240

cagcagauca ugaagaaggg caagggccag auccccguga agaacaacuc cgaggacgac     3300

cgccucugcg ccaccgagaa guacggggcc uacaacaagg cgagcggcgc cuacuucaug     3360

cuggucgaga gcgaggacaa gaaggggaac aagauccgga cccuggaguu cgugccggug     3420

uaccucgcca aggagcugga gaaguccgag gacgccgcca uccgcuacug cgucgagaag     3480

cuggagcuca agaaccccga cgugcgggug cgcaagauca agauggacag ccuguucaac     3540

gucgacggcu uccggaugca ccuguccggg cgcacgggca agcagcucau cuucaagugc     3600

gcggagcagc uggugcugga cgaggagagc cagaagaucc ucaagaagac cgccaaggug     3660

uucgccgacu acaaggagaa caagaacauc aagaucuccg acaagaucga gcugaccgac     3720

gagaugcuga ucaagcucua cgagcuguuc uacaagaagc ugaaggacac cgucuacaac     3780

auccggcuca gcacccaggu gaagacgcug gaguccggca aggagaaguu cgagaagcug     3840

agccuccugg acaagugccu ggugaucgag gagauccucc accuguucca gugccagucc     3900

gucgccgcga accugagcuc caucgggggc gccgccaccg ccgggauccu cgugcugaac     3960

aacgagauca gccgcugcgg caaggucgag aucaucaacc aguccaucac cggguucuac     4020

gagcagguca ucgaccugaa gaacaucuga                                      4050


<210>  1862
<211>  4050
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1862
augcagaagu acuaccuggg ccucgacauc gggaccaaca gcgugggcug ggcggucacc       60

gacgagaacu acaaccugug caaguucggg ggcaagcgca ugugggggau ccggcuguuc      120

gacggcgccc agacggcgga ggagcgccgg augaaccgcu ccaaccggcg ccggcucagc      180

cgccagaagc agcggaucga ccugcugcag gagcuguucg ccaaggagau cgccaaggug      240

gaccccaccu ucuucauccg ccugaacgag ucccggcugu acauggagga caagagcacc      300

ggcaccaagu ucccccucuu cgccgacccg ggguacacgg acaaggagua cuacaaggag      360

uaccccacca ucuaccaccu ccgccaggag cugaucaaga accccgaguc ccacgacauc      420

cggcucgugu accuggccug ccaccacauc cugaagaacc gcggccacuu ccucaucaac      480

gggagcuucu ccgaccugaa gaaccugagc uucgucugcc gggagaugcu cgacgcguuc      540

aacgagcugu ccaaguacca gcugaugauc agcgacaucg acgcccucga gaagauccug      600

uccgaccgca agcagcagaa guccgccaag gugaaggagc uggugcagau cuucgagugc      660

gucccgcggg aggacaagag cgacgccaag aucugcaagg agauccucag caaccugugc      720

aaguuccugg ugggcgcgaa ggggaacauc acccgccucu ucgacaccga cuucgacgag      780

auggagacgu ccagcuucuc cuucggcgac agcaaguacg aggacucccu gcugcccagc      840

cucgaggaga acuaccccga cuacgccaac gugauccaga agaucaaggc cauguacgac      900

uggagcgucc ugguggagau cauggccggc gagaacuacc uguccuucgc caaggugaac      960

gcguacaaga agcacaagga gaaccucagc auccugaagu cccugaugaa gaaguacugc     1020

acccccgacg ucuaccagcg guucuucuac gagaccgacg agaaguccag cuacgggaac     1080

uacaucggcg ccauccacaa caacgggcac agccacacca ugaagaagug cgacgaggag     1140

acguucuaca agucccucag caagauccug gacaagcugg uggugcagaa gaccgaccag     1200

gacacccucg agauccugaa gaaggaggcc aagaaccaga cccugcuccc gcuggcccgc     1260

acgaaggcca acggcgucau ccccaaccag auccacaucc aggagcugcg ggcgauccuc     1320

gacaacgcca agaccuaccu guccuuccug aacgaggccg acgaguacgg caccaucgcc     1380

gagaagauca uccgcaucgc caaguucaag auccccuacu acgcggggcc ccucagcacc     1440

cgccacaagg agcagggcgc caacacgugg aucgugcgga agcaggaggg gcgcaucuac     1500

ccguggaacu ucgaggagau gguggaccug gagcggucca acaacgaguu cauccgccgg     1560

augaccaaca agugcaccua ccugaucggc gaggacgucc ugcccaagaa cagccuccug     1620

uacuccaagu ucauggugcu gaacgagcuc aacaaccuga agauccgcgg gcacaagauc     1680

agcguggagc ugaagcagga gcucuacaag gagcuguucu gcaagaaggc caaggucacc     1740

ggcaaggccc ugcucgugua ccugaagaag aaggacgccg agcugacgcu cgaggacauc     1800

uccggcuucg acgcggacuu caagagcucc cugagcaucu acaucgacuu caagaaccac     1860

auccugcggg agcagaucga cgacgggaag uaccagucca ucguggagaa caucaucaag     1920

uggaagacca ucuucgacgu cgacagcaag augaucgaga agcucaucgc ccgcgaguac     1980

cccaacaugu ucagcuccga gcagaucaag aagaucugcc ggcugaagug ccgcggcugg     2040

gggaacuuca gcucccgguu ccugaacggc auccaggggg agaacaccga gaccaaggag     2100

ugcuucacga ucaucgaggc ccucuggaag accaacuaca accugaugca gcugcucagc     2160

aagcaguuca ccuucaagga cgccaucgac ugcaucaacg cggacaaggu gggcaagauc     2220

gacaagguca ccuacgagaa cacggugaag gaccuggugg ucucccccgc caacaagcgc     2280

gccaucuggc agaccgugca gaucaccgag gagaucaaga aggugaugaa gugcgagccg     2340

gagaagaucu ucgucgagau ggcccggggc ggggagaagg agaagaagcg caccaucagc     2400

cggaaggagc gccugcucaa gcuguacaug gacuacaagg acgagaaccg ggacuggauc     2460

aaggagaucg agucccgcga cgagcaggac uucacgaacc ggaagcugua ccucuacuac     2520

acccagaucg gccgcugcau guacagcggg gaggagaucu cccuggagga gcugaugcag     2580

aagaacagca agugggacaa ggaccacauc uacccccagu ccaagaucaa ggacgacagc     2640

cucgacaacc uggugcuggc ccgcaaggac aucaacaaca acaaguccaa caagcuccug     2700

cccaccggca uccaggacaa gaugaagaag uacuggaaga ugcugcucaa guacggguuc     2760

aucagcaaga agaaguacga ccggcugaug cgcaccgagg acuucuccga cgaggagcug     2820

agcggcuuca ucgcgcggca gaucguggag acccgccagu ccagcaaggc cgucgccgag     2880

cuccuggagc accugaacga gcacacggag aucguguacg ugaaggccga gcucgucagc     2940

gacuuccggc acgacaacaa caugcugaag ucccgccgga ucaacgacua ccaccacgcc     3000

aaggacgcgu uccugaacau cguggugggc aacgucuaca acgccaaguu caccgccaac     3060

ccccgcaacu ggaucaagaa gaaccggaac accaacuaca gcauccgccg gguguucgac     3120

uacaccguga agcgcggguc cacgaucguc uggcagggcg ugaaggacgg gggccacagc     3180

aucgacggca ucaagaagac cauggcccgg aacgacaucc ucuacaccga gcgcaccuac     3240

ugcgagcagg gggagcuguu caacgccacg cugaucaaga agggcaaccc gaacgcgauc     3300

gggcucaaga agggccugga ccccaugaag uacgggggcu acuccagccc caagaccucc     3360

gccuucgccu ucaucgaguu cgacggcaag aaggggcggc agaaccaccu gguggagauc     3420

cccgucuaca ucgccaacau cgcggcccgc cagccggagu acaucaagaa ccaccucgag     3480

gagaagaagg gcuaccagaa cgugaucgug aagaaguacc ccauccagaa gaacgcccug     3540

aucaagcugg acggguaccc cgcccggcuc cgcggcgcca acgagaaguc caucacccug     3600

aagaacgcgg uccagcuggu gcucaagccc gaguaccacg agaccauccg gaacguggag     3660

aaguaccugg aguacaucga caaggacgag agcgucagca aggccgcccg cuucucccac     3720

acggagcugg acaagcucua cgacgagcug ugcuucaagg ccgagaacag caucuacaag     3780

aagcggccgg ccaaccaguc cgcgacccug aagaacgggc agaagaccuu ccucgccauc     3840

accaaccuga aggagaaggc ccaggugauc aacaacauca ucacgauguu ccgcugcgac     3900

gccaccacca ccacgaaccu gaagaccauc ggcggcagcg ccaacgccgg gggcaugcag     3960

gucaacaaga acacccucac cucccagaag guggugcugg ugcaccagag caucacgggg     4020

cuguucggca ccgaggagga gcuccuguga                                      4050


<210>  1863
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1863
augaagaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

gugaccgacg acuacaaggu gccggccaag aagaugacgg uccucgggaa caccgacaag      120

agccacauca agaagaaccu gcugggcgcg cuccuguucg acuccgggaa caccgccgcc      180

gaccgccggc ugaagcgcac cgcccggcgc cgguacacgc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucgc cgaggagaug agcaaggugg acgacuccuu cuuccaccgc      300

cuggaggaca gcuuccucgu gaccgaggac aagcggggcg agcgccaccc caucuucggc      360

aaccuggagg aggaggucaa guaccacgag aacuucccca ccaucuacca ccuccggcag      420

uaccucgcgg acaaccccga gaagguggac cugcgccugg uguaccucgc ccuggcccac      480

aucaucaagu uccgggggca cuuccugauc gagggcaagu ucgacacccg caacaacgac      540

guccagcggc uguuccagga guuccuggcc guguacgaca acacguucga gaacuccagc      600

cugcaggagc agaacgugca ggucgaggag auccucaccg acaagaucuc caagagcgcg      660

aagaaggacc gcgugcugaa gcuguucccg aacgagaagu ccaacgggcg guucgccgag      720

uuccucaagc ugaucguggg caaccaggcc gacuucaaga agcacuucga gcuggaggag      780

aagacgcccc uccaguucag caaggacacc uacgaggagg agcuggaggu ccugcucgcc      840

cagaucgggg acaacuacgc cgagcuguuc cuguccgcga agaagcucua cgacagcauc      900

cugcuguccg gcauccucac ggugaccgac gugagcacca aggcgccccu guccgccagc      960

augauccagc gcuacaacga gcaccagaug gaccuggccc agcucaagca guucauccgg     1020

cagaagcugu ccgacaagua caacgagguc uucagcgacg ugagcaagga cggcuacgcc     1080

ggguacaucg acggcaagac caaccaggag gccuucuaca aguaccugaa ggggcuccug     1140

aacaagaucg agggcuccgg cuacuuccug gacaagaucg agcgcgagga cuuccuccgc     1200

aagcagcgga cguucgacaa cgggagcauc ccccaccaga uccaccucca ggagaugcgc     1260

gccaucaucc ggcgccaggc cgaguucuac ccguuccugg ccgacaacca ggaccggauc     1320

gagaagaucc ugaccuuccg cauccccuac uacgugggcc cccuggcccg ggggaagucc     1380

gacuucgcgu ggcugagccg caaguccgcc gacaagauca cccccuggaa cuucgacgag     1440

aucgucgaca aggagagcuc cgccgaggcc uucaucaacc ggaugaccaa cuacgaccug     1500

uaccucccga accagaaggu gcugcccaag cacagccugc ucuacgagaa guucacggug     1560

uacaacgagc ugaccaaggu caaguacaag accgagcagg gcaagaccgc cuucuucgac     1620

gcgaacauga agcaggagau cuucgacggg guguucaagg ucuaccgcaa ggugacgaag     1680

gacaagcuga uggacuuccu cgagaaggag uucgacgagu uccggaucgu ggaccugacc     1740

ggccuggaca aggagaacaa ggccuucaac gccuccuacg gcaccuacca cgaccuccgc     1800

aagauccugg acaaggacuu ccuggacaac agcaagaacg agaagauccu cgaggacauc     1860

guccugaccc ugacccucuu cgaggaccgg gagaugaucc gcaagcggcu ggagaacuac     1920

uccgaccugc ucaccaagga gcaggucaag aagcuggagc gccggcacua caccgggugg     1980

ggccgccuga gcgccgagcu cauccacggg auccggaaca aggagucccg caagaccauc     2040

cuggacuacc ugaucgacga cggcaacagc aaccgcaacu ucaugcagcu caucaacgac     2100

gacgcgcugu ccuucaagga ggagaucgcc aaggcccagg ugaucgggga gacggacaac     2160

cugaaccagg uggugagcga caucgccggc ucccccgcca ucaagaaggg cauccuccag     2220

agccugaaga ucguggacga gcuggucaag aucauggggc accagcccga gaacaucgug     2280

guggagaugg cgcgggagaa ccaguucacc aaccagggcc gccggaacuc ccagcagcgc     2340

cucaaggggc ugaccgacag caucaaggag uucggcagcc agauccugaa ggagcacccg     2400

gucgagaacu cccagcucca gaacgaccgg cuguuccugu acuaccucca gaacgggcgc     2460

gacauguaca ccggcgagga gcuggacauc gacuaccuga gccaguacga caucgaccac     2520

aucauccccc aggccuucau caaggacaac uccaucgaca accgggugcu cacgagcucc     2580

aaggagaacc gcggcaagag cgacgacgug cccuccgagg acgucgugaa ccggaugcgc     2640

cccuucugga acaagcugcu gagcuccggg cucaucagcc agcggaagua caacaaccug     2700

accaagaagg agcugacccc ggacgacaag gccggcuuca ucaagcgcca gcucguggag     2760

acccggcaga ucaccaagca cgucgcccgc augcuggacg agcgguucaa caaggaguuc     2820

gacgacaaca acaagcgcau ccggcgcgug aagaucguga cgcugaaguc caaccucguc     2880

agcuccuucc ggaaggaguu cgagcuguac aaggugcgcg agaucaacga cuaccaccac     2940

gcccacgacg cguaccugaa cgccgugguc gugaaggccc uccuggugaa guaccccaag     3000

cuggagcccg aguucgucua cggggaguac cccaaguaca acagcuaccg ggagcgcaag     3060

accgccaccc agaagauguu cuucuacucc aacaucauga acauguucaa gagcaaggug     3120

aagcucgccg acgaccagau cguggagcgc ccgaugaucg aggucaacga cgagaccggc     3180

gagaucgcgu gggacaagac gaagcacauc accaccguga agaaggugcu guccuacccc     3240

caggucaaca ucgugaagaa gguggaggag cagaccaucg gccagaacgg gggccuguuc     3300

gacgacaacc ccaagagccc ccucgagguc aucccgagca agcuggugcc ccugaagaag     3360

gcccucaacc ccgagaagua cgggggcuac cagaagccga cgaccgccua ccccauccug     3420

cugaucgugg acaccaagca gcucaucccc aucuccguca uggacaagaa gcgguucgag     3480

cagaaccccg ugaaguuccu gaaggacaag ggguaccagc agaucgagaa gaacaacuuc     3540

gugaagcugc cgaaguacac ccucgucgac aucggcaacg gcaucaagcg ccugugggcc     3600

agcuccaagg aggugcacaa ggggaaccag cuggugguca gcaagaaguc ccaggaccuc     3660

cuguaccacg cgcaccaccu ggacaacgac uacagcaacg aguacgugaa gaaccacuac     3720

cagcaguucg acauccucuu caacgagauc acguccuuca gcaagaagug caagcugggc     3780

aaggagcaca uccagaagau cgaggaggcc uacuccaagg agcgggacag cgccuccauc     3840

gaggagcugg ccgacggguu caucaagcuc cugggcuuca cccagcuggg ggccaccagc     3900

cccuucuccu uccucggcau caagcugaac cagaagcagu acaccggcaa gaaggacuac     3960

cugcuccccu gcauggaggc gacgcugauc caccagagca ucaccgggcu guacgagacc     4020

cgcaucgacc ucuccaagcu gggcggggac uga                                  4053


<210>  1864
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1864
augggcaaga acuacuaccu ggggcucgac augggcacca gcuccguggg gugggccguc       60

accgacgaga aguaccgcau ccugcgggcc aagggcaagg accugugggg cgugcgccuc      120

uucagcgagg cgaagacggc cgccgagcgg cgcacccacc ggaccucccg ccggcgccug      180

gaccgggaga aggcccgcau cgccugccug aaggagaugu ucgcggagga gaucaacaag      240

aucgaccccg gguucuacca gcggcucaac gacagcaagu acuuccugga ggacaagaag      300

gagcaccagc cguacgccau cuucgccgac accggcuaca cggacaagga guacuacgac      360

gaguacccca ccaucuucca ccuccgcaag gcccuccuga acaucgagaa cgacaagaag      420

aaguacgacg ugcggcuggu cuaccucgcg auccugaaca uguucaagca ccgcgggcac      480

uuccugaacg ccaaccucga cgacaagucc ggcgggaacc ugaaggagua caucgagaug      540

cugcaccaga gcauggugaa cauccucaac aucaaccuga agaacgugga gaucgagaag      600

aucgagaaga uccugaaguc cacccaguac agcaacuccg agcggcucga gaaccugcug      660

gagcucuacg agcugagcaa gaacaagaac aagaucgaga ccgagaucuu caaguucauc      720

ugcggccuga aggggacgau cuccaagauc uacgucgagg acaaguucga ggacgaguuc      780

gccaagauga cccucagcuu ccgcgacucc aacuucgacg agaagaucau cgagaucgag      840

gacaaccugg acgacgacaa guacgacaug uuccugcuca ucaagcagau ccacgacugg      900

agcgugcugg ccaacaucau gaacggcgag gaguaccugu ccguggcccg ggucaagcuc      960

uacgacaagc acaagaagga ccuggaggug cugaagaagu acuacaagca gaacagcaug     1020

gaggaguaca acaagauguu ccgccagaug aacgacggca acuacuccgc guacgugggg     1080

agcgucaucu acaaggacaa cuccgugcgg cgcggcugca agagcaagaa ggaggacuuc     1140

uacaagaaca uccucaacac caucaagagc ugggaggacu gcgaggccaa ggaguacauc     1200

accuccgaga ucgacaaggg gaacuuccug cccaagcaga ucacggccag caacggcgug     1260

auccccaacc agguccacaa gaaggagcug aagaagaucc ucaagaacgc cuccgaguac     1320

cugaacuucc ugaacgagaa ggacgagagc ggcuacacca ucuccgagaa gaucgugaag     1380

cuguucgagu uccagauccc guacuacgug gggcccaucg ccuacaacau cggcaacgac     1440

gagagcaagc accggcacaa cauguggucc guccgcaagg agaaggggcc caucuacccc     1500

uggaacuucg agcagaagau cgacaucaag aaguccagcg agaaguucau ccgcaaccug     1560

aucaaccacu gcaccuaccu gaacgacgag gaggugcucc cgaagaacag ccugcuguac     1620

gagaaguuca uggugcucaa cgagcugaac aagcugaaga ucaacggcga gaagaucucc     1680

gucgagcuga agcagaacau cuucaacgac cucuucaaga aggggaagaa ggugaccaag     1740

aagggccuga ucaaguaccu gaaggagcag ggcgagaucg acaacugcga ggagguggag     1800

aucagcggga ucgacggcga cuucacgaac aagcucucca acuacaagaa guucgcggac     1860

aucuucgggg uccagagccu gaccuacgag cagaccgaca ucgccgagaa caucauccgg     1920

uacuccacca ucuacggcga cagccgcaag uuccuggagg agcggauccg cgaggaguac     1980

uccaacgugc ucgacgagaa gcagaucaag cggauccugg ggaugaaguu caaggacugg     2040

ggccgccuga gcaaggagcu ccuggagcug uccggcgucg acaaggagac gggggagauc     2100

gccagcguga ucagccggau guggaacgac aacuacaacc ucauggagcu gaucgccacc     2160

gagcgcuucu ccuacgccua cgagaucgag cagcggagca agaagcugga gaagaccauc     2220

uccaccaucg aguacgagga ccucgacgag cuguacauga gcacgcccgu gaagcgcaug     2280

gucuggcaga ccauccuggu gcucaaggag cuggaggagg ugcugggcug cccgcccaag     2340

cggaucuucg ucgagauggc gcgcgacgug aacgccccca aggaccggaa ggacucccgc     2400

aagaagaagc uccuggagcu guacaagaac ugcaagaagg aggaggggga cuggagcaag     2460

gagcucaacg cccgggagga guccgaguuc aagagcaaga agcuguaccu guacuacaug     2520

cagaagggcc gcugcaugua cuccgggaag cccaucgacc ucaagaaccu gcugaacgac     2580

aaccucuacg acaucgacca caucuacccc cggcacuucg ugaaggacga cagcaucgag     2640

aacaaccugg uccuggugga gaagaacuuc aacgcccaca aguccgacaa cuaccccauc     2700

gagaacgaga uccgccagaa gaugcacagc auguggaaga ugcuccggga cggcaacuuc     2760

aucaccgagg agaaguacuc ccgccugauc cgcaaggagg aguucaccga cgacgagaag     2820

gccaaguuca ucagccggca gaucguggag acgcgccagg gcaccaaggu caucaccgac     2880

cuguucgagc agaccuuccc ggaguccgac aucguguacg ugaaggcggg gaacgucagc     2940

gaguuccggc acaaguucaa gcucaucaag ugccgcaacg ugaacgacuu ccaccacgcc     3000

aacgacgccu accugaacau cguggucggc aacguguacu acacgaaguu caccaagucc     3060

gcccugaacu ucaucaagga cuacaagaag aaccccgaga agaacaagua ccacauggac     3120

cacauguucc aguaccccgu gagccgcggg ggcgucgacg cguggaucac caagggcgcc     3180

gagagcaucu ccauggugcg gaagaugaug gccaagaaca cccccauggu gacgcggaug     3240

aacuacgaga agcacagcgg gcagaagggc gggaucuccg accagaacau cugcagcgcc     3300

aagaucgcca agaagaacgu cuaccucccg aucaagaugg gcgacgagaa gcugaagaac     3360

guggagaagu acgggggcua cggcucccug accgcgaagu acuucuuccu cguggagcac     3420

accaucaaga agaagcgcau ccggaccauc gaggccuacc ccauguaccu ggacgcccug     3480

aacaaggaga acaagcuccu gaacuacugc guccaggugc uggaguacga cgagcccaag     3540

aucaucaugg agaagaucaa ggucaagagc cucuucaaga ucaacgggua cuacuacuac     3600

cugaacgcca aguccgugaa ccgcaucauc uggggcaacg ccacccagcu guuccucgag     3660

aacgacauca acgaguacgu gcggaagauc agcaagaaca ucgagugcau cgacaucaac     3720

uccgagaaca acaucaaggu guacgacauc uucaucgaga aguacagcaa caagcuguuc     3780

uccaaccgcc uggggaaccu caaggaggcg aucauccaga accggaacaa guucaucgag     3840

cugaagauca acgagcagau caacgucauc cuggagcucg ugcgcuacag ccaggacggc     3900

guguacucca ucaaccugag ccagcugggg cucuccagca cguacgccau caaccggacc     3960

uccaaggaga ucagcaagau gaaggagugc aagcugauca accaguccgu caccggccug     4020

uacgagaacg agaucgaccu ccugaccgug uga                                  4053


<210>  1865
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1865
augcagcagu acuaccuggg cguggacaug gggagcgccu ccgucggcug ggccgugacc       60

gacgagaagu accagcucgu gcgcaagaag gggaaggacc uguggggcgu ccggaccuuc      120

gacaucgcgc agacggccga ggugcgccgg gugagccgca ccaaccggcg ccggcagaac      180

cgccggaagc agcgcaucca gauccugcag gagcuccugg gggaggaggu ccugaagauc      240

gacgccggcu ucuuccaccg gaugaaggag ucccgcuacg uggccgagga caagcggacc      300

cucgacggca agcaggucga gcugcccuac gcccuguucg uggaccaggg guucaccgac      360

aaggacuucu acaagcaguu cccgacgauc aaccaccuca ucguguaccu gaugaccacc      420

agcgacaccc ccgacauccg ccugguguac cucgcgcugc acuacuacau gaagaaccgg      480

ggcaacuucc ugcacuccgg ggacaucaac gacgucaagg acauccagag cauccucgag      540

cagcuggaga acgugcugaa ggaguacgug gacgacuggg agcucucccu gaaggacaag      600

gucgacgcca ucaaggagau cuacaacaag gaccugggcc gcggggagcg gaagaaggcc      660

uucaucaaca cgcucggcgu gaagaccaag agcgccaagg cguucugcuc ccugaucagc      720

ggcgggucca ccaaccuggc cgagcuguuc gacgacagcg gccugaagga guccgaguac      780

gccaagaucg aguucgccaa cgccaacuuc gaggacagcg uggaggggau ccaggcgcug      840

cucgaggacc gcuucgccgu caucgaggcc gccaagcggc uguacgacug gaagauccug      900

accgacaucc ucggcgacaa cgccucccug gcggaggccc gcgucaagag cuacgagacg      960

caccacgagc agcuggugga gcucaagucc uucaucaaga aguaccugga ccggaagauc     1020

uaccaggaca ucuucaucaa ccccaacauc gccaacaacu accccgccua cgugggccac     1080

accaagauca acgggaagaa gcaggagcug gaggugaagc gcgccaagcg caacgacuuc     1140

uacgcguaca ucaagaagca ggucaucgac ccgaucaaga agaaggugag cgacaaggcc     1200

gugcucgccc ggcuggccga gaucgagucc cugaucgagg ucaacaagua ccucccccuc     1260

caggugaaca gcgacaacgg cgugaucccc uaccagauca agcugaacga gcuccgccgg     1320

aucuucaaca accuggagaa ccgccugccc guccucaagg agaaccggga caagaucauc     1380

aagaccuuca gcuaccgcau cccguacuac guggggcccc ugaacggcgu gaaccggaac     1440

gggaagucca ccaacuggau gguccgcaag gagggcgagg agggcaagau cuaccccugg     1500

aacuucgagg agaaggugga ccuggaggcc agcgcggaga aguucauccg gcgcaugacg     1560

aacaagugca ccuaccucgu gaacgaggac guccugccca aguacucccu gcuguacagc     1620

aaguaccucg ugcuguccga gcugaacaac cuccggcugg acgggcgccc gcuggaggug     1680

agcgucaagc aggagaucua cgagaacgug uucaagcgga accgcaaggu gacccucaag     1740

aagaucaaga acuaccugcu gaaggagggc gucaucuccg agaaggacga gcucagcggg     1800

cuggccgacg acgugaaguc cagccugacc gccuaccacg acuucaagga gaagcucggc     1860

caccugacgc ugaccgagga ccagauggag aagaucaucc ucaacgugac ccuguucggg     1920

gacgacaaga agcugcucaa gaagcggcug gccgcgcugu accccaacau cgacgagaag     1980

ucccucagcc gcauggccac cuucaacuac cgggacuggg gccgccuguc caagaaguuc     2040

cugagcgaga ucacguccgu cgaccaggag accggcgagc uccggaccau cauccagugc     2100

auguacgaga cccagaacaa ccugaugcag cugcucagcg agcccuacca cuucguggag     2160

gccaucgaga aggagaaccc caagguggac cuggagucca ucagcuaccg caucgucaac     2220

gaccuguacg uguccccggc cgugaagcgc cagaucuggc agacgcuccu ggucaucaag     2280

gacaucaagc aggucaugaa gcacgacccc aagcggaucu ucaucgagau ggcccgcgag     2340

aagcaggaga gcaagaccac caagagccgg aagcaggucc uguccgaggu guacaagaac     2400

gcggagaagu acaagaaccu cuucgagaag cugaacagcc ugaccgagga gcagcuccgc     2460

uccaagaagg uguaccugua cuucacgcag cuggggaagu gcauguacac caacgacgcc     2520

aucgacuucg agaaccucgu gagcgccaac uccaacuacg acaucgacca caucuacccc     2580

cagagcaaga ccaucgacga cuccuucaac aaccuggugc uggugaagaa gggcaucaac     2640

aacgacaaga gcgaccggua ccccaucgac aagaacaucc gcgacgacga gaaggugaag     2700

acccucugga acacgcugcu guccaagggg cucaucacca aggagaaguu cgagcggcug     2760

auccgcagca ccccguucuc cgacgaggag cuggccggcu ucaucgcccg gcagcucguc     2820

gagacccgcc agagcaccaa ggcgguggcc gagauccugu ccaacugguu ccccgagagc     2880

gagaucgugu acuccaaggc caagcacauc acgaacuucc ggcaggacuu cgagauccug     2940

aagguccgcg agcucaacga cugccaccac gcccacgacg ccuaccugaa caucguggug     3000

gggaacgcgu accacaccaa guucaccaac agccccuacc gguucaucca gaacaaggcc     3060

aaccaggagu acaaccugcg caagcuccug cagaaggcca agaagaucga guccaacggc     3120

gucaucgccu ggaucggcca gagcgagaac aaccccggga ccaucgcgac ggugaagaag     3180

gugaucagcc ggaacaccgu ccugaucucc cgcaugguga aggaggugga cggccagcug     3240

uucgaccagc agcucaugaa gaaggggaag ggccaggucc cgaucaagag cuccgacgac     3300

cggcugaucg acaucagcaa guacggcggg uacaacaagg ccaagggcgc cuacuucgug     3360

uucaucaagu ccgugcgccg ggggaagacc aucaagagcu ucgaguacau ccccguccac     3420

cucgccaaga aguucgacug caaccuggag cugcucaagg aguaccugga guccgagaag     3480

gaccugaaca acguggagau ccucaugccc aaggugauga ucaacagccu guucaacuac     3540

aacggcuccc ugauccgcau ccccgggcgg uacgacaaga agucccuccu gaucaacguc     3600

gacgugccgc ugcuccugga gagccagcac aucaagcagc ugaaggucau cgagaaguac     3660

auguacaaga agcgcgugag caagaacucc aacauccucc ugaccaaguu cgccagcgac     3720

cagcugaagg accucgacgc gcuguucgac gugcuguccu acaagcucaa cgagaacauc     3780

uacaacguca ucaacgacaa guacgacaag cuggugaucu gccgcgacaa guucaucagc     3840

cuggacacgg aggugaagug cgagaugauc uucgagcucc ugcaccuguu ccagugcaac     3900

ucccagcucg ccaacaucac caagaucggc gccaccagca aguucggcuc caucagcaug     3960

agcaagaacc ugaaggagaa cgacaagaug uccaucaucc accagagccc cuccgggauc     4020

uucgagcacg agaucgagcu gaccgcccuc uga                                  4053


<210>  1866
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1866
augaagcgca uccugggccu cgaccugggg accgccagca ucggcugggc ccuggugaac       60

gaguccgaga acgagaagga gaaguccgag aucaucaagc ucgggguccg ggugaacccc      120

cugaccacgg acgagcagac cgacuucgag aagggccgcc cgcuguccgu gaacgcggac      180

cggacccuca agcgcggggc ccggcgcaac cugcagcggu ucaagcagcg ccggaagaac      240

cugaucgaga uccucaucaa gaagggcuuc aucaccaaca ucagccugcu gacggagauc      300

ggcuccaaca ccaccuacca gacccucgag cugcgcgcca agagcgucaa ggagaagauc      360

gagaaggagg aguucgcccg ggugcugcuc acgaucaaca agaagcgcgg guacaagucc      420

agccggaagg ccaagaacga cgaggagggc ucccugaucg acgggaugag cguggcgaag      480

gagcuguacg acaacaaccu caccgucggc caguacgugu accagcugcu ggagaagggc      540

gggaaguaca uccccgacuu cuaccgcucc gaccuccaga acgaguucga cgugaucugg      600

aacuuccaga aggaguucua ccccaacauc cugaccggcg agcuguacaa gaagcuccag      660

gggcagggcc agcagaacag ccggaagcug uuccuggcca uccaccagau cuacaccgcc      720

gagaacaagg ggaagcgcga cacggucaag aagcagcacu acaaguggcg gaacgacgcc      780

aucaacaccc agcuccugau cgaggaggug gcguacgugc uggucgagau caacaacaac      840

cucaacaagu ccagcggcua ccugggcgcc aucuccgacc gcagcaagga gcuguacuuc      900

aacaaggaga ccguggggga gaaccucuac aagcagaucc aggagaaccc ccagaccucc      960

cugaagaacc agguguucua ccggcaggac uaccuggacg aguucgagca gaucugggag     1020

acgcaggcca aguucuacag cgagcucacc aaggagcuga aggaggaggu ccgcgacgug     1080

gugaucuucu accagcggaa gcugaagucc cagaagcacc ucaucagcga gugcgaguuc     1140

gagaagcacc acaaggccau ccccaagagc agcccccugu uccaggaguu caagaucugg     1200

cagauccuga acaacaucga guucaagcac aagaagacca aggagcucaa gauccugagc     1260

gaggagcuga aggagacccu cuucgaggag cugaacaucc gcggcaacuu cacguccgac     1320

gccguccuga aguucgugaa gcucgacaag aaggaguggg agcugaacua caagaagggg     1380

aucgagggca accgcaccaa cgaggugcug uacaacgucu accagaagau cgcggaguac     1440

gagggguacg gcuucgacug gagcaagaag uccgccgugg agaucaagga ggagcugaac     1500

accaucuuca gcgagaucgg caucaacucc aagauccucg acuucaaccc cgagaucagc     1560

gggaacgacu ucgacaagca ggaguccuac cagcuguggc accuccucua cagcgccgag     1620

gaggacggcu uccugaagca gaagcugcag gacaaguacg gguucaaguc cgaguacgcc     1680

aacaugcucg ccaacgugag cuuccagucc gacuacggca gccuguccgc gcgggccauc     1740

aagaagaucc ugcccaaccu caucgccggg cacgaguuca gcgaggccug cucccugagc     1800

ggcuacaacc acuccaacag ccugaccaag gaggagcucg ccaaccgccc gcugaagggc     1860

aagcuggagc uccugaagaa gaacagccug cggaaccccg ucguggagaa gauccucaac     1920

cagaugguca accuggugaa ccaggugauc gaguccuacg gggagaucga cgagguccgc     1980

aucgagcugg cgcgggagcu caagaagucc gcccaggagc gcgcccugau gacgaccaac     2040

aucaacgcca gcaagaccaa guacgagagc uuccggaagc ugcuccagaa ggacuucggc     2100

aucaagaacc ccacccgcaa cgacaucauc aaguaccggc uguacgagga gcugaaggcc     2160

aacaaguacc acacgaucua caccaacaag uacauccccc gcgacaagcu guucuccaag     2220

gagaucgaca ucgagcacau caucccgaag gcgaagcugu ucgacgacag cuucuccaac     2280

aagacccugg ccuuccggaa gcccaaccag gacaagggga acaugaccgc caucgacuac     2340

auccucagcu accaguccaa ggacaucgac aacuacaaga gccgcaucga gaaccuguuc     2400

aagaacggca agggggacau cuccaaggcc aagcggaaca agcugcucau ggaggagaag     2460

gacauccccg acggcuucau cgaccgcgac cugcggaaca gccaguacau cgcgaagaag     2520

gccaagcagc ugcucgagga ggugauccgc acggugaaca ccaccaccgg cuccgucacg     2580

aacaagcugc gggacgacug ggggcugauc aacgugaugc aggagcucaa caugcccaag     2640

uucaaggcca ucgacgagag ccugaucgag cagaucgaga agaaggacgg caccuucaag     2700

acccgcauca aggacugguc caagcggaac gaccaccgcc accacgccau ggacgcccug     2760

accguggcgu ucacccgcua caaccacauc caguaccuca acaaccugaa cgccaagagc     2820

gacaagaagg gggccaucua cggcauccag gagcuggaga cguacgucaa cuccaagggc     2880

aagcggaagu ucaucaagcc gaugcacaac uuccgcgagg aggccaagaa gcacaucgag     2940

gacauccuca ucagcuucaa ggccaagaac aaggugguga ccaagaacau caacaagauc     3000

aagagcaagg acggguucau caagaagguc cagcugaccc cgcggggcca gcugcacaag     3060

gagaccgugu acgggaagau caacgaggug gucgugaagg aggagaagau caacgcgaag     3120

uucgaccucg agaagaucaa caaggugacg aagcccaucu accgcaacgc ccugcugaag     3180

cggcuccagg aguucaacgg cgagcccaag aaggccuuca ccgggaagaa cucccugagc     3240

aagaacccga ucuaccugga cgucgcccag accaagaccc uccccgaguc cgugaaggug     3300

caguucuucg aggagaacua cacgauccgc aaggagauca gccccgaccu gaagaucgac     3360

aagaucaucg acaagggcau ccagcggauc cugcucaagc gccuggacga guucaacggc     3420

aagcccaagg aggccuucuc caaccuggag aagaacccga ucuggcucaa caaggagaag     3480

gggaucgcga ucaagcgggu caagaucaag ggcgugaaga acguggagag ccugcacaac     3540

aagcgcgacc accuggggaa cgagauccuc cagaagggca aguccgcccc caacgacuac     3600

gucagcaccg ggaacaacca ccacguggcc aucuaccagg acaagaacgg caagcugcag     3660

gagaaggugg ucuccuucua cgaggccgug gcgcggguga acaacaagcu gcccaucauc     3720

gaccaggacu ucaacaagga ccucggcugg aaguuccugu ucaccaugaa gcagaacgag     3780

auguucgucu uccccagcga ggacuucaag ccgcuggaga ccgaccucau ggacaagaag     3840

aacaacucca agaucagcaa gaaccuguuc cgcgugcaga agcuggggaa caaggacuac     3900

acguuccggc accaccucga gaccaagauc gaggacauca agaagcugcg cgacuucacc     3960

uacauccgga agcgcaaccc cgaguccauc aagaacauca ucaaggugcg gaucaaccac     4020

cugggcaaca ucguccacgu gggggaguac uga                                  4053


<210>  1867
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1867
augagcaaga aguacuaccu gggccucgac aucgggacca acuccguggg cugggccguc       60

accgacgaga acuacaaccu gugcaaguac gccgggaagc gcaugugggg cauccggcug      120

uucgagggcg gggagacggc ggagcagcgc cggaucagcc gcuccaaccg gcgccggcuc      180

agccgcaaga agcagcggau cgaccugcug caggagcugu ucgccgagga gaucgccaag      240

auggacccca ccuucuucau ccgccugaac gagucccggc ugcaccucga ggacaagagc      300

accggcgaga aguucccgcu guuccgcgac aaggacuaca ccgacaucca guacuacaag      360

gaguacccca cgauguacca ccuccggaag gagcucaucg agaacuccaa ggugcacgac      420

auccgccugg uguaccuggc cugccaccac auccucaaga accgggggca cuuccugauc      480

aacggcagcu ucuccgacgu caagaagcug agcuccgugu gcgagagcau gcucaacucc      540

uucaacggga ucagcaagua ccagaucgag aucaacgaca ucgagcgccu ggaggagauc      600

cuguccgacc ggaagaagca gaaguccgug aaggccaagg agcucaucca gaucuucgag      660

ugcggccccu gcgaggacaa gaccgacgag aagaccugca aggcgaucgu cagcaaccug      720

ugcaaguggc ugaccgggcu caagggcaac aucaacagca ucuucgacau gcaguucgag      780

gagaucgaca cguccagcuu cagcuucacc gagcccaagu acgaggacac caucuccgcc      840

gcccuggagg agaaguaccc ggacuacgcc gacgugaucc agaagaucaa ggcgguguac      900

gacuggagcg uccuggugga gaucauggcc ggcgaggacu accucuccuu cgccaaggug      960

aacgccuaca acaagcacaa ggagaaccug ugcauccuga aggaggucau ccgcaaguac     1020

ugcgacagcg agaucuacca caaguucuuc aacggguucg acgagaagaa cggcuacggg     1080

aacuacaucg gcuccaucua caagaacggc cacagcuacg ccaugaagaa gugcuccacc     1140

gaggacuucu acaagacgcu cgggaagauc cuggacaaga uccagcccga gaaggaggac     1200

gagaacgugc uggaggagcu caagguggag gucaagaacc agacccugcu gccccucgcg     1260

cggaccaccg ccaacggcac gauccccaac caggugcaca uccaggagcu gcgcgccauc     1320

cuggacaacg ccaagaagua ccucccguuc cugaacgagg aggacgagua cgggaccgug     1380

gccgagaaga ucaucagcau cgcgaaguuc cggauccccu acuacgucgg cccccugucc     1440

acccgccaca aggagcaggg ggccaacagc uggaucgugc gcaagcagga gggccggauc     1500

uaccccugga acuucgagga gaugguggac cucgagcagu ccaacaagga guucauccag     1560

cgcaugacca acaagugcac guaccugauc ggcgaggacg uccugccgaa gaacagccuc     1620

cuguacuccc gguacauggu gcugaacgag cucaacaacc ugcgcauccg ggggaacaag     1680

aucagcguga agcugaagca ggagcuguac aaggagcugu ucugcaccaa ggccaagguc     1740

accggcaagg gccucuucaa cuaccugaag aaggaggacg aggagcucgc ccuggaggac     1800

aucuccgggu ucgacaucga cuucaagagc ucccugacca gcuaccucga cuucaagaag     1860

cagauccugg gggaggagau cgagaaggac aaguacaagg gcaucgugga gaacaucauc     1920

aaguggaaga cgaucuacga cgacgacucc aagaugauga agaagaugau cgagcgcgag     1980

uaccccaacg ucuucagcaa ggacaagauc aagaagaucu gccgguucaa guacagcggc     2040

ugggggaacu ucucccugag cuuccucaac ggcauccgcg gggccgaccg ggagaccggc     2100

gaguccuuca ccaucaucga ggcgcugugg aagaccaacu acaaccugau gcagcuccug     2160

agcaagcagu ucacguuccg cgaggagauc gaccugauca acgccgagaa gguggggaag     2220

aucaacaagg uguccuacga caacaccguc aaggaccuca ucgugagccc cgccaacaag     2280

cgggccaucu ggcagaccgu gcagaucacc gaggagauca agaaggucau gaagugcgag     2340

cccgagcgca ucuucaucga gauggcccgg ggcggcgaga aggagaagaa gcgcacggug     2400

ucccggaagg cgcgccugcu ggagcucuac gccgccugca aggacgacgu gcgggacugg     2460

accaaggaga ucgaggaccg cgaggagcgg gaguucaaca gcaagaagcu guaccuguac     2520

uacacccaga uggggcgcug cauguacucc ggcgaggaga ucaacaucga cgagcucaug     2580

cagaagaaca gcaaguggga caucgaccac aucuacccgc aguccaagau caaggacgac     2640

agccuggaca acaugguccu ggccaagaag gugaucaaca acuccaagca gaacaagcuc     2700

cugagcuccg acauccgcau caagaugaag aaguacugga acgcgcugua caaggggaac     2760

uucaucagca agaagaagua cgaccggcuc acccgcacgg aggacuucac caacgaggag     2820

cuguccggcu ucaucgagcg gcagaucgug gagacccgcc agagcagcaa ggccgucgcc     2880

gagcugcuca accggcugua cgagcacucc gagaucgugu acgugaaggc cggccugguc     2940

agcgacuucc gccacgacaa caagcuccug aagucccggc gcaucaacga cuaccaccac     3000

gccaaggacg cguuccugaa caucguggug gggaacgucu acaacgccaa guucaccagc     3060

aacccccgga aguggaucaa ggagaaccgc ggcaccaacu acuccauccg gcgcguguuc     3120

gacuacaacg ugaagcgggg gaacacgguc guguggcagg gcguggagga cgggggccac     3180

agcaucgacg agauccgcaa gaccauggcc cggaacgaca uccucuacac cgaguacacc     3240

uacugcgaca agggcgagcu guucaacgcc acgcugauca agaaggggaa cgacaaggcc     3300

aucaaccuca agaaggcgcu ggacuccguc aaguacggcg gguacaacag ccccaagacc     3360

uccgccuucg ccuucaucga guucgacggc aagaagaagg agcgcaagaa ccacaucgug     3420

gagauccccg uguacaucgu caacaucgcc aagcggcagc cggacaccau caaggaguac     3480

cuggagcaga agaaggggua caagaacgug guggucaaga aguaccccau caagaagaac     3540

gcccucauca agaucgacgg cuaccuggcg cgccugcggg gcgccaacga gaccgacauc     3600

cuccugaaga acgccgugca gcugauccuc aacgacaagu acuacgagau cauccgccgg     3660

guggagaagu accuggagua caacguccgc uacgaggccg agagcauguu cgagggguuc     3720

gaccacgcgg accugaacuc ccucuacgac gagcugacga gcaagcugga gaccuccauc     3780

uacaagaagc gccccgccaa ccagcucaac gcccuggaga agggcaagga gaaguucaag     3840

aacauccaga cccugcggga gaaggcccag aucaucaagg agaugcucau cauguaccgc     3900

ugcgacgccg cgaccguggc caaccugaag gccaucgggg gcagcgccaa cgccgggucc     3960

augaagauca agaagaacac gcugaccagc cggucccucg ugcuggucaa ccagagcauc     4020

accggccugu ucgagaccga ggaggagcuc guguga                               4056


<210>  1868
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1868
auguacaaga aguacggcau cgggcuggac aucggcacca gcuccaucgg guggagcgug       60

gucgacgagu acggccacau cauccgcgug aaggggcaga ccggccucgg cgugcggcug      120

uuccgcgagg ggcagcccgc cgccgaccgg cgcacguucc ggaccacccg ccggcgccug      180

ggccggcgcc gguggcgccu ccggcugcug cgcgagaucu ucgacccgua caucgcggcc      240

caggacccca acuucuucgc ccggcagaag gccuccgcca ucgcgcccgg ggacagccgc      300

uucaaggccg ucuaccggcu guucaacgac cagaccgacc gccaguucua cgacaaguac      360

cccacggugu accaccuccg gcagaagcug augaccgaga agcgccaguu cgacauccgg      420

gagaucuacc ucgccgugca ccacaucguc aaguaccgcg gccacuuccu gaccgggggc      480

gccgcgaaga acuucaagcc gggcaagcug gaccucaaca ccuacuucgc cacgaucaac      540

gcccagcugg ccgccaucuu cauggacucc cagguggagc ugccccagcu cgacugggac      600

gcggugguca gccugcugac caacgaccag gaguccccca gcgaccgcca gaaggcccuc      660

accaagaugc ugguggccca gggggacgcc acccagaagc gguucuacac ggaguucucc      720

aaggcccugc ucggccugaa ggcgaagcug uacgugcuca ccggguucga gggcgacaag      780

accgaccaga aggcccugac ccugagccuc gagacgcugg acgaccacca ggacgacguc      840

acccagcugc ucggcgacga gcagcgccag cugguggagc ugcucgcccg ggugcagucc      900

gccauccagc uggccaucau cauccccaac gggaagaccu ucagcggcca gaugguccag      960

caguaccaga cccacuacga ccagcugcgc cuccugaagg acuaccggga gcagcugacg     1020

gaccagaagc accgccgggc gcucaagcag gccuacgccg acuaccuggc caacgaccag     1080

gaccaggacg cccuggugaa ggcgguggag aagguccuca agcagaacca gccgcugucc     1140

accguggggg ugcgccugca gaccgccauc gacgccggcg acuuccuccc caagcagcgg     1200

accaaggcca acagcgucau cccccaccag auccaccagc aggagcugga ccgcaucauc     1260

gaggcgcagu ccguguacua ccccuggcug gccgaggaga acccgguggc cgcccaccgg     1320

agcuacgccc ccuacaagcu cgacgagcug gucgacuucc gcgugcccua cuacgucggg     1380

ccccuggugc agccgcaggc ggacaaggcc gccaaggaga cgaaguucgc cuggaugacc     1440

cggaaggagg cccaguccac cacccccauc acgcccugga acuucgagca ggagguggac     1500

cgcaacgcga gcgccaacgc cuucauccag cggaugaaga ccaccgacac cuaccugcuc     1560

ggcgaggacg uccugcccaa gaacucccug cucuaccagc gcuucgaggu gcugaacgag     1620

cugaacaacg ugcgggucga cggcaagcuc cugacgaucg ccgagaagca gcgccuguuc     1680

caggaccucu ucguggaccg gagccaggcc agcgugacca ucgcgcagcu gcaggccaac     1740

cugaucgccc acggggccgc gaaggaccgc gucgagauca agggccucgc cgacccgaag     1800

cgcuuccugu ccagccugac caccuaccac gacuacgccc agaucauccc cgaggcccuc     1860

gagcagcccg acaagcgggc cgacaucgag aagaucauca acugguccac gaucuucgag     1920

gacaccagca ucuucaagga gaagcugcag gagaucgcgu ggcugacccc cgaccagcgc     1980

cggcagcucu cccagaagcg cuaccggggg uggggccagc ugagcgacaa gcugcucacc     2040

cagcugcgcg acgccaccgg gcaguccgug auggaccugc ucuggacgga gccgaacaac     2100

uucaugcaga ucauccggcg caccgaguuc cagcaggcca ucgcccgggu gaaccagacc     2160

gaccugaagc agcaggaccu ggccaccacg aucaacgacc ucuacaccag cccccagaac     2220

aagaaggcga uccgccaggu ccugcuggug guggccgaca uccagaccgc caugcacgag     2280

gucgcccccu ccuggcuguu caucgaggcc gcgcggggcc ccgagcagaa cccgaagcgc     2340

aagcuggccc ggcagcagca gcugcucgag gccuacaaga ccagcgccaa ggagaucgug     2400

aacgaggcgg ugaaggagaa gcugcugucc gccaucgacg accaccagaa guucgaggac     2460

caggucgugc ucuacuucca gcagaacggc cuggacaugu acacggggaa gccccuggac     2520

uucgagcgcc ucagcaagua ccacaucgac cacaucaucc cccaguccgu ggucaaggac     2580

gacagccugg acaaccgggu gcugaccacc ccggaggaga acgagcgcaa gggcgcccgg     2640

gugcccgggc cccucuacgc cucccagcag ggccgcuggc ggcugcugca gcgcgccggg     2700

cucaucagcc agcggaagcu ggaccaccuc cuccugcgcc ccgaggagcu ggaccagcag     2760

gcgaccggcu ucgucgcccg ccagcucgug gagacgcgcc aggugaucaa gcugaucacc     2820

aacauccugu cccagcagua cgacccggag cagaccaagc ucgucagcgu gaaggccggc     2880

cugucccacc aguuccggac ccggcugaac cuccccaagc ugcgcgagcu gaacgacuac     2940

caccacgccc acgacgccua ccucgcggcc cggaucggga cguaccugcu gcagcgcuac     3000

cccaaccucg agcgguucuu cguguacggc caguaccaga agacccgcuu cgaccugaag     3060

cggcacuuca acuucaucgg ggagaugcug aagggcaagc ccgaggacgc cgucaucgug     3120

aaggccccgc acagcgagac cgugaucugg gacaagcagg acgaggucaa gcagcucaag     3180

aagaucaugg aguaccgcca ccuccuggug acccacgagg uguacgacaa ccacggcgcc     3240

cuguucaacc agacgaucca ccccgcgaag aaccccaaga accggaagcu gaucccccgc     3300

aagaccaccc agagcaccga gcuguacggg ggcuacuccg gggagacgac cggcuaccuc     3360

gccaucgccc ggaccgucac caagaacaag aucagcuacc aggugcugcg cgugccgacg     3420

cggcuggucg ggcgccucca gcgguccagc gaguccgccc agcgccagau ccugaccgac     3480

ugguucgccc ccagcuucgu gucccggggc aagcagcagc ccuucgagau cgugcugcgc     3540

cacgucuuca agaaccagac cgugaucgac gacauccacg agcagagcca ccgcuucggc     3600

cuccgguccg acaccuacua cugcaaccag cagcagcugg ugcugagccu cgagcagcag     3660

cgcaugauca ucgaccagaa gcugaacaac acgaacaagc ugauggucgu guacgaggcg     3720

gugugcgacc aggucgccaa guacuucccc cucuaccgga ugaaccacuu caaggagucc     3780

cugcuggagg ccgggccgaa gcucgccgag cugcccauca acaccgugca gagcaagaac     3840

ggcaagcuga agcucgggca gcgcuccgug cugcugcaga ccaugcaggg ccuccacgcg     3900

aaccccgaga ccaaggaccu gagcaucauc gggugcccca aguacuucgg caagcugcag     3960

gucuccagcg gcaucaagcu gacggagaac gccgugcuca uccacgaguc cccgaccggg     4020

cuguuccagc ggcgcguccg gcugagcgac cucuga                               4056


<210>  1869
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1869
augaagaucc ugggccucga ccuggggacc aacagcaucg gcugggccgu ggucaacgag       60

gccgagaacg agcgcgaguc cagcuccauc auccggcugg gggugcgcgu gaacccccuc      120

accgucgacg agcagaacaa cuucgagaag ggcaagccga ucacgaccaa cgcggaccgg      180

acccugaagc gcagcaugcg gcgcaaccug cagcgguaca agcuccgccg ggccaaccug      240

gugucccugc ucaagaccca gggguggauc acggacgaga cccugcugag cgagcagggc      300

aacaacucca ccuucgagac cuaccgccuc cgggccgagg ccgccacgaa ggagaucacc      360

cuggagcagu ucgcgcgcgu gcugcucaug cugaacaaga agcggggcua caagagcucc      420

cgcaagauga agcacgacga ggacgggagc uucgucaaca accuggacau cgcccgggag      480

cucuacgacu ccggccugac ccccgggcag uacgccuaca accugcucaa ggacggcaag      540

aaguacaucc ccgacuucua ccgcagcgac cugcaggagg agcuggacaa gaucugggcc      600

uaccaguacg aguaccacga gaacauccuc acccacuccc ugcggagcgc gaucaucgac      660

caguccaaga cggggaccac caagaucuuc cugaagaccc acggcaucuu cacggccgac      720

aucaagggca accgcaacga gcggaacaag cagcuguucg agcuccgcug cggggcccug      780

gagcgggccc ucaccgccga ggagcuggcg accgugaucg ccgaggugaa cggccagauc      840

aacagcucca gcggguaccu gggcgccauc uccgaccgca gcaaggcccu cuucaucaac      900

caccagaccg ucggccagua ccugaugcag cagcuggacc gcgaccccaa caagagccuc      960

aagaaccagc cguucuaccg gcaggacuac cuggacgagu ucgagcagcu gugggagacg     1020

caggcccacu accaccccga gcucaccgcg gcccugaagc acgagauccg cgacaucauc     1080

aucuucuacc agcggcgccu gaagucccag aaguggcucg uggccaacug ccccuucgag     1140

caccggaagg ugugccccaa gagcuccccg cuguuccagc acuuccgcau cuggcagguc     1200

cugaacaacg ugaccgugac cgucgacaac aagacguacg ugcucaacga ggagcagaag     1260

uccauccuga gcaaggagcu gcucuacaag gagaagauca gcaagcggga cgcccugaag     1320

augcuguucg ugaaggccaa gggggucgac augaacuucg cgucccugga gggcgaccgc     1380

accaugagca accuccugaa ggccgcccag gagaucgugu ccaucagcgg gcacggcgac     1440

uacgacuucg ggaagaugcc cgccgacgac auccacaaca ucauccgguc cguguucggc     1500

ggccuggggu ucgccaccga cuggcucaac cuggacaccg acgcggacga ccuggagcag     1560

gagagccaga ugcgccucug gcaccugauc uacuccuucg agggcgacaa cagcacgacc     1620

ggggacacca agcuggucga gcggcucaug ucccugcuga accuccccca ggaguacgcc     1680

cgccuguucg ccaacgugca ccuggaggac gacuacugcg gcaucagcuc caaggcccuc     1740

aagcggaucc ugcccuucau gaaggcgggg cacaucuaca gcgaggccug cgcccuggcc     1800

ggcuacaagc acuccgagca gagccucacc cgcgaggaga acaagaaccg gccgcugaag     1860

gaccaccugg agcagcuccc caagaacagc cugcgcaacc ccguggucga gaagauccug     1920

aaccagaugg ugaacgucgu gaacuccauc aucgacacgu acggccggcc cgacgagauc     1980

cgcaucgagc ucgcccggga gcugaagaac aacgcgcugg ggcgccaggc caucuucgag     2040

gccaucaacc gcaacaccaa ggagagcgag cggauccagc agauccucaa gaaggaguuc     2100

ggccugaccc acgugucccg caacgacauc auccgguacc gccuguacca ggagcucagc     2160

gggaacggcu acaagacccu guacuccaac aucuacaucc cgcaggagaa gcuguucagc     2220

aaggagaucg acaucgagca caucaucccc caggcccgcc ucuucgacga cuccuucagc     2280

aacaagacgc uggaguaccg ggacgucaac aucaagaagu ccaacaccac cgccaucgac     2340

uucgugcgga acgaguacgg ggagaacgag guggagcagu acaagaagcg cgucaacgac     2400

cugcucaaga gcggcgcgau cucccaggcc aaggccaaga agcugaagau gaccgaggcc     2460

gagaucccca acgacuucau cgagcgggac cugcgcaaca cccaguacau cagccggaag     2520

gcccaggaga ugcuccgcga cguggcgcgg guggucacgg ccaccaccgg cuccgugacc     2580

gaccgccugc ggcaggacug gcagcuggug gacgucaugc aggagcucaa cugggacaag     2640

uacgagcgcc aggggcugac gaccgugauc aacggccggg acgggcagca gauccgccgg     2700

aucaccgacu ggaccaagcg caacgaccac cggcaccacg ccauggacgc ccugacgauc     2760

gcguucacca agcccgccua caucagcuac cucaaccacc ugaacgcccg cuccgaggac     2820

gacauccgca gcaucgagcg gcgcuuccug gagcgggacg ccaacggcca ccuccgcuuc     2880

aucuccccga ucgcccacaa cuuccggagc gaggcgaagc gccaccugga gaugauccug     2940

gugagcauca aggccaagaa caaggucgug acccggaaca acaacaccac gaagaccaag     3000

ggcgggcgcc ggaaccgcau ccagcucacg ccccggggcc agcugcacga cgccaccgug     3060

uacgggucca uccugcuccc ccacaccaag accaucgccg ucgacggcaa gaugaccgcc     3120

gagaucaucg gccaggugca gaaccccgug uuccgccagg cgcugagcca gcggcuggag     3180

gccugcggga acgacccgaa gaaggccuuc accggcaaga acucccucga gaaggccccc     3240

gucuggaucg accaggccca cacggagcac gugcccgcgg aggugaccau ccagcgccug     3300

gagccccagc acaccauccg gcgcgccguc aacaaggacc ugaacaucaa caaggugcuc     3360

gacaagggga uccagcacau ccugcaggcc cggcuggacg ccuacggcgg ggacgcggcc     3420

aaggccuucg ccaaccucga caacaacccg aucuggcuga accaggagaa gggcaucgcc     3480

aucaagcgcg ugaccacggc ggagaacauc agcggggagc cccugcacca caagcacgac     3540

aaggagggcc ggcccauccu cgacaccaac ggcaagccga ugcccuccga cuucguccgc     3600

cugaugaaca accaccacgu ggccaucuuc caggacgccg acgggaaccu gcaggagcac     3660

aucgugagcu ucuacgaggc cgucgcccgc gugaccgagc ucggccugcc cgugaucgac     3720

aaggaguaca acaaggacaa gggguggcag uuccuguuca cccucaagca gaacgaguac     3780

uucgucuucc ccaacccggc gcagggcuuc aaccccgacg agaucgaccu gacggacgag     3840

cggaacuacu cccgcaucag ccccaaccug uaccgggugc agaagauguc cagcaaguac     3900

uacguguucc gccaccaccu cgagaccacc gucgaggaca agaaggagcu gcaggggauc     3960

accuggaagc ggauccaguc ccugaacaac cucaagggca ucgugaaggu gcgcaucgac     4020

cacaucggcc ggaucgucca gaucggggag uacuga                               4056


<210>  1870
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1870
auguucagcc agcagaacaa ccuguacugc gagucccuca gcaagcugua cuacaacauc       60

accaagaacu uccuguccaa gaacgaggug ugccgcaucu acgagaucuu cuucugcagc      120

auccucaccu acuuccugaa gaaguacaac cacgacaucu ucaagaaguc cagcacgaug      180

aagaccaucc ugggccucga ccuggggguc accuccaucg gcugggccgu gaucgugacc      240

gacgacgaca acacgcccau caagauccug gggaugggca gccggaucau cccgcucucc      300

accgccgaca aggaggaguu ccagaagggg caggcgauca gcaagaacca gucccgcacc      360

aucgcccgga cccagcgcaa gggcuacaac cggaagcagc ugcgcaagga ggagcugaag      420

aagauccucg gcgagcuggg gaucacgccc agcaaggagc ugcucaaccu gcccuccauc      480

gagcugugga agcuccgggc cgacgccgcc agcggcaacg cgccccuguc cgccgagcag      540

cuggggcgca ucuucuacau gcucaaccag aagcggggcu acaagagcgc ccgcuccgag      600

gccaaccagg acaagaagga caccgacuac gucgcggccg ugaaggggcg gcaccagcag      660

cugaaggaga agggccagac caucggccag cacuucuaca ccgagaugcu gacgcucgag      720

gagaacagcg ggcacuucca ggugaagaac aacgucuacc cgcgcgaggc cuaccuggag      780

gaguucaacg ccaucaucgc cgcgcagaag ccccagcacg ccuuccugac cgacgacgug      840

guggaccggc uccgcgacga ggucaucuuc uaccagcgga agcugaaguc ccagaagggc      900

cuggugagca ucugcgaguu cgagggguuc gagcgcaccu ucuucgacaa cgugaagcag      960

aaggacaaga ccaucuucac gggccccaag gucgccccca agagcucccc gcuguuccag     1020

cugugcaaga ucugggagac cgugaacaac aucaaccuga aggugaagaa caccgacggc     1080

agcaagaaca agugggccga cuacgucccc acccucgagc agaagcagga gaucaucgag     1140

caccuguuca agaacgccgg gaucucccug gacgcgcucc ggaagauccu gggcuucaac     1200

aaggacgaga ucuucgugaa caagcagauc gagaaggggc ugcagggcaa cacgaccuac     1260

gccgagaucc acaagauccu cggggacagc aagcccgagc ugcugggcuu caacaucaac     1320

aucgagcccu ccaagcacag cgcccuccug guggacaagc gcaccggcga gauccuggcc     1380

gagaaggagg ggcagcaggu cuccgccgag aucgagaagg agaacuucua ccagcucugg     1440

cacaccaucu acagcaucaa ggaccuggac gagugcgcgg ccgcccugau caagcgguuc     1500

aacaucgacc cgaacguggc cgcccagcuc gcgaagaucg acuucuccaa gggcgccuuc     1560

gggaacaaga gcaacaaggc caugcgcaag auccugcccu accugaugca gggcuacaac     1620

uacgccgacg cgugcucccu ggccggguac aaccacagcg acucccagac gaagaaggag     1680

aaggaggaga acguggucga cgacaagcuc aagcugcugg agaagaacag ccuccggcag     1740

cccguggugg agaagaucau caaccagaug auccacgucg ugaacgccau caucgacaag     1800

uacggcaagc ccgacgagau ccgcgucgag cuggcccgcg accugaagaa guccaaggac     1860

gagcggaacg aggccgagcu ccagaacgcg agcaacaaga agcugaacga ggagguggcc     1920

aaccgccuga aggagcucgg ccugccggug accaagcggu ucguccagaa guacaaguuc     1980

aucuuccccg ugcgcgaccg gaagaucaag gaggcccagc uggugaacca gugcaucuac     2040

ugcggggaga ccuucaacgu caccgaggcc cucuccggcg acaacuucga cguggaccac     2100

aucaucccca agagcaugcu guucgacgac ucccagacga acaaggugcu gguccaccgc     2160

aagugcaacg ccaaggacaa gagcaacaag accgcguacg acuucaucgc cgccaagggg     2220

gagaacgagc ucgccaucua ccuggaccgg guggacgacu gguucaagcg cggcgugcug     2280

agcuacggga agaugcagcg gcucaagguc uccuacgagc aguacgccga gcgcaagcag     2340

cugaagaagg agaccgaggc ggacaucaag cugugggaga acuucaucga gcgggaccuc     2400

cgccagaccc aguacaucgc caagaagucc aaggaccugc ugaagaagau cugcaacaac     2460

gugacgguga ccgagggcaa gaucaccgcc cagcuccgga accugugggg cugggacgac     2520

guccugauga accuccagcu gcccaaguac aaggcccugg ggcagaccga gaucaaggag     2580

uggacgagcg agcacggccg ccggcuccac aagaaggagg agaucaccaa cuggaccaag     2640

cgcgacgacc accggcacca cgcgaucgac gcccuggugg uggccugcac caagcagggg     2700

uucauccagc gcaucaacac ccugaacagc uccgacacga aggacgccau ggaccgggag     2760

aucaaggccg cgaaggagaa guacaacgag cucuacgaca agaagcugcu gaccgagacc     2820

aacgagcagg ucaagaacca caccgagcgc uucaacaagc ucgagacgua ccucaagagc     2880

cagcagccgc cccuguucac cacccgcuac guggagcagg aggccgacaa gaucuucgug     2940

uccuucaagg ccggcaagaa ggccgccacc uacggcaucc ggaagaucaa gaaggacggg     3000

aagaaggucg uggugcagga gaagaucguc gugccccgcg gcgcgcucca cgagcagagc     3060

guguacggga agaucaagac gcaggagaag gacaagcccc ugaaguaccu guucgagaac     3120

ccggagacca ucgucgacaa ccggaucaag auccucguga aggagcgccu ggacaaguuc     3180

gagggcgacg ugaagaaggc ccugaccucc cucaagaagg agccccuguu caucgacaac     3240

gagaagaaca cccugcucga gaaggccgcc ugcuacacgg acgccaccgu ccugaaguac     3300

aagcugcagg cgcucaaggc caaccaggug aacgacaucg uggacgaggg ggucaagcgg     3360

aucgugaagg cccgccuggc ccagcacaag aacgagaagg aggcguucaa ggacaccgug     3420

ugguucaacc aggagaagcg gauccccauc gucagcgugc gcguguucgc ccggcccgac     3480

gccuccaagc uggccaucau caagaccgac gagaacaaca agccgaucgg cuucgcccuc     3540

acgggcaaca accaccacau cgcgaucuac aaggacaaca ccgggaagcu gguccagcac     3600

aucugcaccu ucuggagcgc cguggagcgc aagaaguucg gcgugcccac cgucgugaag     3660

aacacgggga cccugugguc cgagcuccug cacaaggacc ugcccggcac cuuccucgac     3720

aagcugccgc aggacgggcu ggagcucgag uacagccugc agcagaacga gauguucgug     3780

cugggccucc ccaaggaguc cuucgaggac gccaucaaga acaacgaccu gccccugcuc     3840

agcaagcacc uguaccuggu cugguccauc agcgacaacg acuacugguu ccggcaccac     3900

cucgagacca agaacagcga gcugaagucc acgaccggcg ccaaggagag caagcgcuac     3960

uaccgguucc aguccguggg gaccuucacc aaggagaacc ccaucaaggu gcagcugaac     4020

caccucggcg agaucacgaa gaucggggag ccguga                               4056


<210>  1871
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1871
augaagaagc gcaaccggga ggaguacuac gugggccugg acaucgggac cagcuccguc       60

ggcugggccg uggccacccc cgacuaccgc aucccgaagu uccgggggca caagaugugg      120

ggcagccgcc ucuucgcgga ggggaagcgg gccgccgagc gccggcaguu ccgcacgaac      180

cggcgccggc uggcccgccg gcgcgaccgg cugcagaucc ucgaggagcu guucgccgag      240

gagauggcga agguggacaa caccuucuuc augcgccuga aggaguccaa guucuuccuc      300

gaggacaaga ccgucaagga gaagcacauc cuguucagca ccgacaaguu cgacgccgac      360

cgguucuaca aggacaaccc cacgaucuac cacgugcgca agcggcugau ggaggaggag      420

ccccaggaca uccgcgagcu guuccucgcc gugcaccaca uccugaagua ccggggccac      480

uuccucuucg ccggcucccu ggagcagaac ggggcgagca ucgaggagcu gguccgcacc      540

cuccugggca agcaggggcu ggccauccuc gacgacacca ucgacuccaa cgagauggug      600

aacauccuga agaacaccgg cgagaccaag agcgaccgca agaaggacgu gcaggagcug      660

uggaaccccc ucaaggacaa ggucaagaag gcccaggccg ccgagauggc ggggcuggug      720

cugggccuca aguccgacau cagcaagcug uucgccaacu ccgacuacaa ggaccuggac      780

agcuccauca agaacaucaa cuucagcucc ggcgacuacg aggagaagcg ggacgacuac      840

gccaacagca ucggggacga caucgugcuc cuggacacca ugaaggcccu guacgacacc      900

cucauccugu cccagauccu gcagggcaag cgcagcaucu ccgccgcgaa ggucgaggac      960

uacaacaccc acaaggagca gcuccgggcc cugaaggcca uccugaagcc gcacaagcgc     1020

cucuacaacg agauguuccg ggagaacaac aagaaggacc ugagcaacua cguggccuac     1080

aucagcuccu acagcgaggg gaagcgcgug aaccgggagg gcuucuacaa guacguccag     1140

gccgugcuga agcaggucga cgacuccgac agcaagcagg cgauccucga ggccauccgc     1200

cuggagacgu uccugccccu ccugcgggug aaggacaacg ccgugauccc ccaccagauc     1260

caccaggccg agcuggaggc cauccucgag accgcgcgca agaaguacaa cuuccugaac     1320

gacguguccg acggcgucag cgugaaggac aagaucaucc agcuguucac cuuccggauc     1380

cccuacuacg uggggccgcu caacccccac uccggcgaga ccgucgccga ccgcaagcag     1440

gggacgggcu gggugguccg gaagcagcgc gggaaggugu accccuggaa cuucgccgac     1500

augguggacg ucaaggccag cgcggaggcc uucaucacca accugaccaa caagugcacc     1560

uaccugaucg gcgaggacgu gcugcccaag cggucccucc uguacagcga guucaugcug     1620

cucaacgagc ugaacaugau caaguacgac ggccacgagc ugccgguggc caucaagcag     1680

cucuacaucg agcaggucuu caagcaggag cacaagaagc agacgaaggg gcgcgugcug     1740

gacuuccuga agcggaacgg ccucaucacc ggguccgcca ccgccgugau caccggcaug     1800

gacgacgcga ucaaggggga ccugcagagc uaccgcgaca ugguccggau cuucggcgcc     1860

ggcuucgacc acuccauggc cgagcgcauc auccgcuggg ugacgcuguu cggggaggcc     1920

aaggacaucc ucgagaacaa gauccgggag guguacggca acagcaucuc cgacagccag     1980

augcgcgcca ucaagaagcu gaaguacgcg gacugggggc ggcuguccca ggccuuccuc     2040

aucgccguca agggcugcga caagagcggg guggagcgca acaucaucac gugccugcgg     2100

gccuccagcc agaaccugau ggagauccuc agcgaccagg gcaccuacau caagcagauc     2160

aaggccugga accaccagca caccacgaac augauggaca cccuggacua ccagcuggug     2220

gacgaccucu acgucgcgcc cuccgugaag cgcagcaucu ggcagucccu gugcaucgug     2280

gaggagcugg ucaagaucau gggccaggcc cccaagaaga ucuucgugga ggugacccgg     2340

accaagcagg ccgagaagac gaagaaggac agccgcaaga agcggcucgu cgagcuguac     2400

accgccaucg ggaaggaggg cgcggccugg aagaaggaga ucgaggccca ccaggccggg     2460

gacuuccgcc ggaagaagcu guaccucuac uacacccaga ugggccgcug cauguacucc     2520

aacgagccca ucgccaugga cgcgcuguuc accagcgccu acgacaucga ccacaucuac     2580

ccgcaguccc ugacgaagga cggcagcugg gacaaccucg ugcuggugaa gucccagcug     2640

aacaaggaga aguccgacca guacccccuc cggcccgcca uccgcgagaa gaacaaggcc     2700

cacuggcagu accugugccg ggccggguuc aucagcgaga agaaguacga gcgccugaug     2760

cggaccaccg agcucaccgc ggaggagcug agcggcuuca ucgcccgcca gcuggucgag     2820

accucccagg ccacgaaggc cgugaccggg cuccuggagc ggaucuacac caacaccgag     2880

aucugcuucg ugaaggccga gacggucagc caguuccgcc accagcacaa cuucaucaag     2940

auccgggagc ugaacaacua ccaccacgcg aaggacgccu accucaacau cguggugggc     3000

aacgucuacc acgagaaguu caccaagaac cccugggagu ucaucgcccg cuccaccgag     3060

cgccggccgu acaaccugaa ccgcauguac gacuucgacc ugcucaagca ggggcgggug     3120

aucuggcagg uccccaagag ccuggagacc gugcaggcca ugaugaaguc caacgacgug     3180

cgcaucacga agaagcugcu cgugcagaag ggcgagcugu acgacgcgac caucaccaag     3240

gcccacaagg ccaaggaggg caccuacuuc ccccugaagg gggccgacgc ccggcuccgc     3300

gacguccacc gguacggcgg guacgagaag aucaagaucg cguacuacag cguguucagc     3360

ugccgcgaga aggccuccaa cacgauccac acccugauca uccccauccc gguguacaag     3420

gccggccugc uccagaccga ggccgaccug accgccuacg cguggcagac gaagcagaag     3480

accaagccca ucaaggacau cuacagcgcc aucaccccca ucuacaagaa gcugugcauc     3540

aacaccgucg ugaagaucga cggguucaag uacuuccucg gcggcaagac gggggacuac     3600

auguggcugg accccgccau cccggugguc cuggacgagc aggccgccgc guaccucaag     3660

gccaucucca aggugggccg ggccgacgac guggagaaca ccgcccaggc caucgaccug     3720

aucaccgcgg aggccaacga ggccuucuac gacguccugc ucaagaagau ggaggccaag     3780

aucuucgcga aggccuacca gaccaucgug gagaagcugg ccagcgagac ggcccgcgag     3840

cgguuccgcg agcuguccau cggggcccag ugcagcugcc uccuggagau ccugaaccuc     3900

cuggcgaaca acaagaccac cuucaccuuc uucaaggaca agaugggcgu gacgaugucc     3960

cggcgcaagc agcccaugga ccugaccaag cucgaggagu gcaucgucua cgagaccagc     4020

aucaccgggc uguacgagaa ggccgugucc cugcucuga                            4059


<210>  1872
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1872
augggcaagc gcuaccacau cgggcuggac aucggcacca gcuccaucgg gugggccgug       60

auggacgacg acuucaagau caugcggguc aagggcaaga aggggaucgg cgugcgccuc      120

uucgaggagg gcaagaccgc cgcggagcgg cgcgggcacc ggacgacccg ccggcgcuac      180

agccggcgca aguggcggcu gaaccugcuc gaggagaucu ucgaccccga gaucgccaag      240

guggacccga ccuucuucgc ccgccugaag gaguccaacc ugagccccaa ggacgaccgg      300

aagcaguacu ccggcaaccu caucuucccc gagaaggcca aggaggagau ccgcgagugg      360

accgagaagu accccacgau cuaccaccuc cggaacgccc ugaugaccga gaaccgccag      420

uucgacaucc gggaggucuu ccucgcgcug caccacaucg ugaaguaccg cgggaacuuc      480

cuguucaacg gcaacuucca gaccggggag gugaaccuca gcgaccgguu cgacgacauc      540

aacuccgccu ucaucagcgc cugcggcgag gaggaggccc cgcagauccc cuccgagcug      600

uacaacgaca uccaggagcu gcuguucgac aagaacaccc gcaagcugga caaccagaag      660

cagcuggcga cgaagcucag ccaggacaac gacaaggacu gguccaccca guucagcaag      720

gcccuguucg gcuacaagac caaguucaag gucgugcugc aguccaacga ggaccucgac      780

gacuuccagc uggacagcga cgacgccgac gacaagaucg ccuccaucag cgagcagauc      840

gacgacgacc ggggguccau ccuggacaac aucaagaucc ucuacagccg caucacccug      900

aacgagaucg ugcccgacgg caagacgcug ucccagagca ugaucgagaa guacaaccuc      960

caccgcgacc accugaagga gaucaaggac cagcugaagc acaccgacaa cugggucaag     1020

uucgacgacc ucuacagcca guacgugaac gggaagcugg aguccgaccg gcacaagaag     1080

cugagcuccg acgacuucua caagaaggug cucacccugu ucgacggcga gcugaagacc     1140

cgcgucaagg aguggcagga gaagggcgag uuccucccca agcagcggac ggggagcaac     1200

ggcgugaucc cgcaccagcu gcaccagaac gagcuggacc agaucaucga gaaccagucc     1260

aaguacuacc ccuggcucgc cacccugaac cccaacgaga agcgccagcg gguggcgaag     1320

uacaagcuga gcgagcucgu cgccuuccgc auccccuacu acguggggcc gauggugacc     1380

aaggaccagu ccgacuucgc cuggaugguc cggaaguccg acgagaagga gaucaccccc     1440

uggaacuucg accagguggu ggaccgcgac aaguccgccg aggaguucau caaccggaug     1500

acgaccaagg acaccuaccu gaucggcgag gacguccugc ccgccaagag ccuccuguac     1560

gagaaguuca ccgugcugaa cgagcucaac aacauccgca ucaacgggaa gccccugacg     1620

accgacgaga agcaccaggu guaccgggac cuguuccaga acgcgaaguc caucagcacc     1680

aagaaguucc acaacuaccu ggucugcaag cucaacaagc cgaaccaccu gaacaucacc     1740

ggcuucuccg accugacgaa guucaacagc ucccucgaca gcuacaucga ccugaagaag     1800

aucuucgccg accagaucga cgaccagucc cugcaggacg acuucgagaa gaugauccgc     1860

uggagcaccg uguucgagga caagcagauc uucgagcgga agcucuccga gaucaacugg     1920

cugaccgacc aggagcgcag cgaggucgcc aagcugcggu accgcggcug ggggcggcuc     1980

agcaagcgcc ugcuggccgg cauccacgac gagaacgggc agcggaucau cgacgcccuc     2040

uggaacuccc ccaagaacuu caugcagauc caggcggagg uggccuucaa caagcagauc     2100

aacgaccuga acaccaagga gcugagcgac aacaacgugg aggacguccu cgccgacgcc     2160

uacacgagcc cccagaacaa gaaggccauc cgccaggugc ugcugguggu cgaggacauc     2220

cagaaggcga ugcacggcga cgcccccacc agcaucuccc ucgaguucgc ccgcgaggag     2280

caggagagcu cccggacccg cagccggcgc gccgcgcugu ccgccaccua ccggcagauc     2340

agcgacgaga ucguguccgc cacggugaag ggggagcugg gcaaggccac cagcaucucc     2400

gaccgccucu accuguacuu cacccagggc gggcgggaca uguacaccgg cgagccgauc     2460

aacaucgacg agaucagccg cggguacgag aucgaccaca uccugcccca guccuucauc     2520

aaggacgaca gccucgacaa ccggguccug gugaagggcg ccaucaacaa cgggaagucc     2580

gcgaacuacg ugauggacca gagcugggcc cccgaccgcc uggccuggug gaagaagcuc     2640

cuggacaacc ggcugaucag ccgcaagaag uaccaguccc ucacccugac gcccggcaag     2700

aucaacaagu acagccagaa cggcuucauc aaccggcagc uggucgagac ccgccaggug     2760

aucaagcucg uggccaacau ccugaacgac caguacucca acgacgacac caagaucauc     2820

gucgugaagg ccgcgcugaa cagccagcuc cggggguucc uggagcuguu caagaaccgc     2880

aacaucaacg acuaccacca cggcauggac gccuaccucu ccaccuucau cgggaacuac     2940

cuguacaaga aguacccgaa gcugcggagc uacuucgugu acggcgaguu caagaaguuc     3000

uccggcaagc agaaccucaa gagcuucaac uuccugcacg accuggagga cgacuccauc     3060

aacaucauca gcaacgacga cggggagauc gucggcaacc accacgagau gaucgaggag     3120

cucgagaagg ccuaccagua caaguacaug cugguguccc gcgagguguu cgagaagacg     3180

ggggaccugu ucaacgucac cguguacagc cggaaggcca agaagacccu cauccccauc     3240

aagcaggaca agcccaucaa caucuacggc ggguacuccg gcaacaacga cgccuacaug     3300

gcgaucguga agcugcccgg caagaagggg aagccggaca ccuacaagau cgucggcauc     3360

ccccugcgcu ucgccgggga gcucgagcgg gccaagcagc ugggcgagag cgccuaccgc     3420

gagaagcugu ccgcggugau cacgccccag uucaccaagg agaagaagga ccgcaagacc     3480

ggggagauca ccaagguggu cgacgacuuc gagguggugc ucgaccgggu cccguaccgc     3540

cagcuguuca aggacagcga caagugguuc augcugggcu ccgccgccua ccaguacaac     3600

gccaagcagc ucgugcugaa caacgacgac gugaagcugc ucaacgagca gggccggaag     3660

aagcuggagc ugcccaacga gaagcucagc uccgcccuga acgacgucuu cgaccacauc     3720

auccagauca ugaacagcaa guucgggcug uacgacaaga acggcuuccg cacgaagcuc     3780

accgaggcgg ccagcaucuu cuacgaccug ccccugcggg ccgaguacaa cgggaacaag     3840

aaggugaccg acggcaagcu cgaggugcug gacaacaucc ugaucgggcu ccacgccgac     3900

cccuccuucg gcaaccugaa gcagcugaac augagcaccc cguucggcca gaugcagacg     3960

aagggcgggg ucgugcucaa cgggaacucc acccugaucu accagagccc caccggccug     4020

uucgagcgcc gggugaagcu caaggaccug uccaaguga                            4059


<210>  1873
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1873
augaagaaga uccugggccu cgaccugggg accaccagca ucggcugggc cuucaucaac       60

gagggggaga acgacaacga gacguccagc aucaucaacg ccggcgugcg caucgucccc      120

cugaccaccg acgaggaggc ggacuucaag aaggggaaca ccaucuccau caacgccgac      180

cggacgcuca agcgcggcgc ccggcgcggc cugcagcggu ucaagcagcg ccgggacgcc      240

cugcuccaga gcuuccgcgg gaucaacuuc aucuccagca acuacgugua cgccgagacc      300

ggcaagcaga ccaccuucuc cagcuacaag cugcgggcga aguccgccac ggagcuggug      360

accaaggagg agcucgucca ggugcugcug augcucaaca agaagcgcgg guacaagagc      420

ucccggaagg ccaagaccgc cgaggagggc gacgcgaucg acgggaugaa gaucgccaag      480

gagaucuucg agaacaaccu gaccccgggc cagugggugu acaacagccu guccaucggc      540

gggaaguuca uccccgacuu cuaccgcagc gaccuccaga aggaggucga gaagaucgug      600

cgguuccaga accaguucca ccccgagcag augaacgaga agcugcugga gaacauccag      660

ggcaaggccc gcacggugac cucccaguac uucagccgga uccucaacau cccccuggcc      720

gagaacaagg ggacccgcga ggagaccaag cugcagcacu acaaguggcg gaacgaggcc      780

cucacggagg agcuggaccu gaagguccuc gcguucauca ucaccgagau caacaaccag      840

aucaaccagu ccaacggcua ccugggcgcc aucagcgacc gcuccaagga gcuguacuuc      900

aaccaggaga ccguggggca guuccaguac aaccagcuca aggagaacgc ccacaccaag      960

cugaagaacc agguguucua ccggcaggac uaccuggacg aguucgagaa gaucugggag     1020

acgcagagca aguucuaccc ggagcucaac gacgagcuca agagcgaggu ccgcgacauc     1080

accaucuucu accagcggaa gcugaagucc cagaagcacc ucgugagcuu cugcgaguuc     1140

gagaagggcc acaaggccau ccccaagucc agcccccugu uccaggaguu ccgcaucugg     1200

cagaacauca acaacaucgu ggucaagaac gagaccacca aggagcccuu ccugcucggg     1260

gaggaccuga agaaggugau cgcccaggag cuguccuuca aggagagcau gacggacucc     1320

cagcuccuga ccuucugcga ccugaagaag ggcacccaca ccgugaacuu caagaagauc     1380

gaggggaacc gcacgaacac cagccucuuc aaggcguucg agaagauccu gguccuggag     1440

ggcuacgacg agcucgaccu guccaagcug gacgccagcg acaucaaguc caucaucaag     1500

agcgccuucu ccgaccucga gaucagcacc gccauccugg acuucgaccc gaccauccag     1560

ggcaacgacu acgacaagca guccuacuac cagcuguggc accuccugua cagcuccgag     1620

gaggacgacu uccugaagaa gucccugaug gacaaguucg gguucaagga gagccaccuc     1680

cccuuccugc ugaacaucaa ccuccaggcc gacuacggca gccugagcgc gaaggccauc     1740

aagaaggugc ugccccaccu cauggacggg cacaucuacg acaaggccug cacgcuggug     1800

ggcuacaacc acuccagcuc ccugaccacc gaccagaaca acgagcgggu ccucaaggac     1860

acccuggagc ugcucaagaa gaacagccug cgcaaccccg ugguggagaa gauccugaac     1920

cagaugauca acgucaucaa cgccaucauc aaggacccgg ccauggggaa gcccgacgag     1980

auccgggugg agcucgcgcg cgagcugaag aacaacaacg agcagcgguc cgagaugacg     2040

aaggccauca acaagagcac caccgagcac gagcagaucc gcaagcugcu ccacaccgag     2100

uucggcaucc accgggucac ccgcaacgac aucauccggu acaagcugug gaaggagugc     2160

gacggcaucu cccuguacac ggggaagccc aucgagccca gcaagcuguu caccaaggac     2220

uacgacaucg agcacaucau cccgaagucc cgccuguucg acgacagcuu cuccaacaag     2280

accaucugcg agcggcagcu gaacaucgac aagagcaaca agaccgccua cuccuucuuc     2340

caggagaagc ucagcgccga ggaguucgac caguucgaga agcgcgugaa gucccuguuc     2400

ggcaagauca gccggacgaa gcagaacaag cugcucaugg cgaacaacga gauccccgag     2460

ggguucaucg accgccagcu gcgggagacc caguacaucg ccaagaaggc caaggagauc     2520

cugcucgagg ugucccgcaa cgucaccgcc accaucggca gcgugacgga caagcugcgg     2580

gacgacuggg agcuggugga cgucaugaag gagcucaacu gggagaagua cgacaagcug     2640

gggcugaccu acaucgagga gggcaagaac ggcgagcgcc ucuacaagau caaggacugg     2700

accaagcgga acgaccaccg ccaccacgcc auggacgcga ucaccguggc cuucacgaag     2760

cccgccuaca uccaguaccu gaacaaccug aacgcccgca ccaacaccga caagaagggg     2820

aacagcauca ucggcaucga gaacaaguac cucaagcggg acaagaacaa caagcugcgc     2880

uucaucgagc ccaucccgaa cuuccgggag guggccaaga agcagcugga guccauccuc     2940

aucagcuaca aggcgaagaa caaggucgug accaagaaca agaacgugac gaagaagucc     3000

aacgggacca accagaagau ccagcugacg ccccgcggcc agcugcacaa ggagaccguc     3060

uacggcaagc uccagcagua cgugaccaag gaggagaaga ucaacgccaa cuucaccccc     3120

gaguacguga ccaaggucgc caagaaggag uaccgggagg cccugcugaa gcgccucauc     3180

gagaacgaga acgaccccaa gaaggccuuc accgggaaga acgcgcugag caagaacccg     3240

aucuacaucu cccuggagaa caacauccuc gugcccgaga aggugaagau cgucuggcuc     3300

aagagcgacu acacgauccg gaaggacauc acccccgacc ugaagaucga caaggugauc     3360

gacaucggcc ugaagaccau ccugcagaac cgccugaacg aguucaacgg ggacaccaag     3420

aaggccuucg ugaaccucga cgagaacccc aucuggcaga acaaggagaa gggcaucgcc     3480

aucaagcggg ucacgaucuc cggggugagc aacgcccagg cgcugcacac caagaaggac     3540

caccugggca acgagauccu cgacgagaac ggccagccgc ugcccgugga cuucgucucc     3600

accgggaaca accaccacgu ggccaucuac cgcgacgaga agggcaaccu gcaggaggag     3660

guggucagcu ucuucgaggc cgugacccgg aacaaccucg ggcuguccau caucaacaag     3720

aaccacgaga agggcuggga guuccuguuc agcaugaagc agaacgaguu cuucaucuuc     3780

cccacggacg gguucaaccc gcacgagauc gaccuccuga acaccgagaa cuaccaccug     3840

aucucccgca acauguuccg ggugcagaag aucgccacca agaacuacau guucaaccac     3900

caccucgaga ccaagagcgu cgacggcgag augcugaaga cgaagaagca gcuguccaag     3960

aucagcuacc acuucaucca gacccccucc uaccucaacu acaucaucaa gauccgcauc     4020

aaccaccugg gccagaucgu gcagcugggg gaguacuga                            4059


<210>  1874
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1874
augaagcagg acuacuacau cggccuggac auggggaccg agagcguggg cugggccguc       60

accgacgugg acuacaaccu caucaagaag cgcgggcagg acuacugggg cguguaccug      120

uucgacgagg cccagacggc gcaggggcgg cgcgccuucc ggaccaaccg ccggcgccug      180

gcccgggucc gccaccggcu caaccugcug caggagcugu ucgacuccga gaucagcgcc      240

aaggacuucg gcuucuucua ccgccuggag aacuccaagu acuucgacga ggacaagccc      300

gagaucgccc ggggcaaguu ccuccucuuc aacgacaagg acuucgggga caaggaguac      360

uacaagaacu acccgaccau cuuccaccuc cgcaacgcgc ugcggaccca ggagaucaag      420

gacgugcgcc uccuguaccu ggccgugcac cacauccuca agaaccgggg ccacuuccug      480

uucggggagc aggacuucaa cgucaacgac gccagcgacg uguccggcga guucgagaag      540

aucaaccgcu uccugagcga gaacgacggg gccggcuucg accgggagaa gauccccgag      600

aucuucgaga cgcucugcaa cgagaaccug gcgaagcgcg agcggaagga gaagauccug      660

gagcucgccg gcaacuccaa gaacaagcug cugggggagg ccgugaacgc cgucgugggc      720

cucaacuuca gccuguccaa gauguucggg gaggaccgcg acuuccaggg cgagaacaag      780

augagcuuca ccgacuccag cuucgaggag aagcuggcca aggcgcggau ggcccucgac      840

gacgacgagg ccgacuuccu ggagacccug aagaaggugc aguccgucgc cagccuccac      900

aacauccugc acggcaagga guucaucucc gaggccaaga ccgaggcgua cgagacgcac      960

cgccgcgacc ugagcgagcu caagaaguac gugcgggaga acugccccga gaaguacgcc     1020

cugguguucc gccggcaggu caagaagggg aagaagaaca ccuccggcgc cgacgagaac     1080

gagaaggacg acaagaaggu gaacaacuac gccgccuaca ucgggaugga caagggcaag     1140

ggguucgaga agugcaagaa ggaggacuuc uacgcguucc ugaagaagga gaucggcauc     1200

aacgacgaga gccuccuggc caagaucgac gagggcaucu uccugcccaa gcagaucucc     1260

gccgagaacu gcgacauccc guaccaggug caccucgccg agcugaaggc cauccuggcg     1320

aacgccgaga acuacuuccc cuuccucaag cagaaggacg agaacugcga gaugaccguc     1380

agcgagaaga ucaucagccu gaugaaguuc cgcgugcccu acuacguggg gcccuucacc     1440

gacaaggaga cgcgguucuc cuggaugauc aagaaggagg gccugagcac ccagaagauc     1500

accccgugga acuucgacga cgucguggac cgcgacgccu ccgagaagaa guucaucgag     1560

cggaugacca acuucugcac guaccucacc ggggagaagg ugcugcccgc cagcuccuuc     1620

cuguacagcg aguacgcguu ccucaacgag cugaacaacc ugaaggucaa cggcaccaag     1680

gaccugcgcg ugaagaacau ccucuucgac uucgccaaga ccaacaagaa gcugacgcug     1740

aagaagugcu gcgagcagcu cgucaagaac gggcugcugc ccgagggcuc caaggcccgg     1800

gacguguuca gcggcaccga cggggacuuc aagaacuccc ucagcuccua caucgacuuc     1860

aagcgcauca ucggcgacaa ggccgaccug uaccgggaca ugugcgagaa gaucauccug     1920

uggaucaccg ugaucagcga caaggagcgc cucgucaaga ucaucaagga caaguacggg     1980

accgugcugu ccgacgagga gaucaagggc cugaaggccc ucaacuacac gaaguggggg     2040

cggcugagcg agaagcugcu cgacggcauc uucgagaccg acuccgaggg cgaggugggg     2100

aacgucagca ucaucgagaa gaugcgcacc gacaccgaga acuucaugga gcugcugucc     2160

aacaagcaca acuacaagcg ggcgaucgag cucgccaaca gcgaguccau cguggacgag     2220

cgcgugacgu accgggccgu cgaggagcug uacugcagcc ccgccgugaa gcgcagcauc     2280

ugguccgccc ugaagaucgu gcgggagauc gucaaggugc agggccacag cccgaagaag     2340

aucuucgugg agaccacccg cgagaagccc gacgucaccc gcaaggggca gcggacggug     2400

ucccgcaagc agcagcuccu ggacaaguac cgggagaacc ugcgcaacga gaccgagcuc     2460

aucaagaaga ucgaggagac cgaggacagc aaguucaacu ccgacaagcu guucuucuac     2520

uucacccaga ugggcaagga cgcguacacg ggcgagaaga ucgaguucga cgacguguuc     2580

aagaccgaga aguacgacau cgaccacauc uacccccgga gcaagaucaa ggacgacucc     2640

cuggacaacc gcguccucgu gcggcgcaag aucaaccaga gcgugaagaa ggacuccuac     2700

ccccugagcg ccgagauccg gaaccgcaug gaggggcugu ggcggcagcu ccgcgacaag     2760

aagcugaucu ccgacgagaa guucuaccgg cugacccgca gcaccccgcu caccgagaag     2820

gagugcggcg acuucaucaa cgcccagcug aucgccacgu cccagagcgc caaggcggcc     2880

auccagcugc ucaagcggau gcugcccgag uccgagaucg ucuacagcaa ggccgggaac     2940

gugaacgagu ucaagaagcg ccuggaguuc gugaagaucc gggagcucaa cgaccugcac     3000

cacgccaagg acgcguacuu caacaucguc gugggcaacg uguucaacac caaguucaac     3060

cacaaccccg ccgucuucca gcgcuccaac gggaccggca acuacgacuu cgagcggcug     3120

uacgacuucg acaucaccaa cgccuggaag aagggcgacg agaaccgcau ccggagcgug     3180

gccgagaaga acacgugccg cgugguccgc uucagcaaga ccgagaaggg gcaguucuac     3240

gacgccacca ucuaccccgc gggcaagaac gacaagcucg ccccggugaa ggggaaggcg     3300

ccccugaacg acaccuccaa guacggcggg uacgacaagg agacgaccgc cuacuucaug     3360

cuggucaaga gccgggacaa gaagaagaag gacaagaccc uccugucccu ggagugcgug     3420

accgugcucg ccgacaagaa cuuccgcacg gccgaggaca agcugaagua cugcgagaag     3480

gagcugggcc ucaaggagcc cgagauccug aucgagaaga ucaagcugaa cacccucuuc     3540

aagaucaacg gcagcuacgc cuggauccgg gggcgcacca acgaccggcu ggugcugugc     3600

aacgccaacc agcucuggcu ggacaacggc uccgccaagg agcugaagaa gaucagcaac     3660

cacuuccgcg acgucaagaa guacaacaac aaggaccucc ccgugcggga ggaggaguuc     3720

uccaaggagc agaaccuggc ccuguacgac cucuucaccg agaagcucgg gggcaagauc     3780

uacgcggggc ugccggugca gggccagauc aagaaccugg agaagggccg cgacaaguuc     3840

gaggcccugg agcugaagga ccagcuguuc guccugaugg agauccugaa guucaugcag     3900

ugcaacagca ugacguccaa caucaagcuc aucgggggcg ccgagaacgc cgggaagaac     3960

cggaucagca agcugaucca ggacuccgac gugaagauga ucuucugcag ccccaccggc     4020

uacuucugca agguggucga cuucaagcgc uuccuguga                            4059


<210>  1875
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1875
augaagaaga uccugggccu ggaccugggg accaccagca ucggcugggc cuucaucaag       60

gagcccgaga aggacguggu cggguccgag aucguggaca ugggcgugcg caucgucccg      120

cugagcuccg acgaggagaa cgacuucgcc aaggggaaca cgaucagcau caacgcggac      180

cggacccuca agcgcggcgc ccggcgcaac cugcagcggu ucaagcagcg ccggaacgcc      240

cugcucgaga ucuucaagga gaagaagcug aucuccacca acuucaagua cgccgaggac      300

ggccccagcu ccaccuucag cacgcugaac cuccgcgcca aggcggccaa ggagaagauc      360

gagcugcagg accuggugaa ggugcuccug cagaucaaca agaagcgggg guacaagucc      420

agccgcaagg ccaaguccga ggaggacgac ggcagcgcca ucgacuccau ggggaucgcg      480

aaggagcugu acgagaacga ccucaccccc ggccaguggg ucuacgaggc ccugcagaag      540

gggcggaaga acgugcccga cuucuaccgc agcgaccugc aggaggaguu caagaagauc      600

gugaacuacc aguccgaguu cuucccggac aucuucaacg ccagcuucgu cgaggacugg      660

augggcaagg ccuccacccc caccaagcag uacuucaaca agaagggcgu gcagcucgcc      720

gagaacaagg ggaagcggga ggagcgccgg cugcaggagu acaaguggcg cgcggaggcc      780

gugaacuuca agaucgaccu gagcgagauc gcccucaucc ugucccagau caacagccag      840

aucagcaacu ccagcggcua ccugggggcc aucuccgacc ggagcaagga gcucuacuuc      900

aagaaccuga cggucggcca guaccuguac cagcagauca agaagaaccc ccacacccgc      960

cucaagggcc agguguucua ccggcaggac uaccuggacg aguucgagcg caucuggucc     1020

gugcagagcu ccuucuaccc ccagcugaac gacgcccuca agcgggaggu ccgcgacauc     1080

accaucuucu uccagcgccg gcucaagagc cagaagcacc ugaucuccaa cugcgaguuc     1140

gaggaccacc acaagguggu gcccaagagc caccccgucu uccaggaguu ccgcaucugg     1200

cagaaccuga acaaccugcu gcucaucaag aaggacaacc ugaacgagaa guucgaccug     1260

gagcucgagu ccaagaucgc gcuggccaac gagcuggccu ucaagcggga gcucaacgug     1320

aaggacgccc ugaagauccu ggggcucaag cccaacgagu gggaguucaa cuucaccaag     1380

aucgagggca accgcacgaa ccaggccuuc uucgacgcgu ucgccaagau caucgagcug     1440

gaggacgggg agcccaucga ccugggcgac cucaaggccg acgacauccu ggaccaguuc     1500

agcgaggccu uccugcggau cgggaucgac accgagcugc uccaggugaa cuccgacauc     1560

gagggcgccg aguacgagaa gcagagcuac auccaguucu ggcaccuccu guacuccagc     1620

gaggacgacc agaagcugaa gcugaaccug auccgcaagu ucggcuucaa gccggagcac     1680

gcgaagaucc ucgccuccau cagccugcag gacgaccacg ccucccugag cagccgggcc     1740

aucaagaaga uccuccccca ccuccagucc gggcugaucu acgacaaggc gugcaccuac     1800

gccggcuaca accacagcuc cagcuucacc aaggacgaga acgagaagcg cgagcugcgg     1860

gccgagcugg agcugcucaa gaagaacucc cugcgcaacc ccgucgugga gaagauccug     1920

aaccagauga ucaacguggu caacgccauc cucaaggacc ccgagcuggg gcggccggac     1980

gagauccgcg uggagauggc ccgggagcug aaggcgaacg ccgagcagcg caagaacaug     2040

acgagcaaca ucgccuccgc cacccgggac cacgacaagu accgcgagau ccucaagagc     2100

gaguucggcc ugaagcgggu caccaagaac gaccugcucc gcuacaagcu guggcuggag     2160

accgacggga ucucccucua cacgggcaag cccaucgagg ccagcaagcu guucuccaag     2220

gaguacgaca ucgagcacau cauccccaag gcgcggcugu ucgacgacag cuucuccaac     2280

aagaccaucu gcgagcgcca gcucaacauc gacaaggcca acgugaccgc cuucagcuuc     2340

cugcagaaca agcuguccgc cgacgaguuc gagcaguacc agagccgcgu gaagucccuc     2400

uacggcaagc ugagcaaggc caagauccag aagcugcuca uggcgaacga caagaucccc     2460

gaggacuuca ucgcccggca gcugcaggag acccgcuaca ucagcaagaa ggccaaggag     2520

auccuguucg agaucucccg gcgcgucagc gugacgaccg ggaccaucac cgacaagcuc     2580

cgggaggacu ggggccuggu ggagaucaug aaggagcuga acugggagaa guacgacaag     2640

cucgggcuga cguacaccau cgagggcaag cacggggagc gccugaacaa gaucaaggac     2700

ugguccaagc ggaacgacca ccgccaccac gccauggacg cgcucaccgu cgcccugacc     2760

aagccggccu acauccagua ccugaacaac cucaacgcca agggccugaa caacaagaag     2820

ggcacggagg uguucgccau cgagcagaag uaccugaagc gggagaacgg gaagcucugc     2880

uucaucccgc ccaucgagaa cauccgcagc gaggcgaaga agcaccuguc ccggauccug     2940

gugagcuaca aggccaagaa caaggucgug accaucaaca agaacaagac caaguccaag     3000

gccggccuca acgagcagau cgcccugacg ccccgcgggc agcugcacaa ggagaccgug     3060

uacggcaaga gcuuccacua cuccaccaag uucgagaaga ucggcgccag cuucaacguc     3120

cagaagauca acaccguggc gaagaaggag gagcgggagg cccuccugaa gcgccuggcc     3180

gagaacggga acgacccgaa gaaggccuuc accggcaaga acacccucaa caagaugccc     3240

aucuaccugg accuggggaa gaacaucaag cucuccgaga aggugaagac ggucgugcug     3300

gagcagaacu acaccauccg gaagaacauc gaccccgacc ugaaggugga caaggucauc     3360

gacgugggca ucaagcgcau ccucgagagc cggcuggagg aguucggggg caacgccaag     3420

cuggcguucu ccaaccucga ggagaacccc aucuggcuga acaaggagaa gggcaucagc     3480

aucaagcgcg ugaagaucuc cggggucagc aacguggagu cccugcacgu gaagaaggac     3540

cacuucggcg agccgauccu cgaccaggag gggaacgaga uccccgucga cuucgugagc     3600

accggcaaca accaccacgu ggccaucuac gaggacgaga acgggaaccu gcaggaggag     3660

gucguguccu ucuucgaggc cgugguccgg cagaaccagg gccugcccau caucaagaag     3720

aaccacaccc ucggcuggaa guuccuguuc acgcugaagc agaacgagua cuucguguuc     3780

ccgagcgacg acuucgugcc cgccgacguc gaccucaugg acgagcagaa cuaccaccug     3840

aucagcccca accuguuccg cgugcagaag aucgcgcgga agaacuacgu guucaacaac     3900

caccucgaga ccaaggccgu cgacaacgac cugcugaagu ccaagaagga gcucagcaag     3960

aucaccuacc acuucuacca gacccccgag caccuccgcg ggaucaucaa gauccgcauc     4020

aaccaccugg gcaagaucau ccagaucggg gaguacuga                            4059


<210>  1876
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1876
augaacaaga aguacuaccu gggccucgac aucgggacca acagcguggg cugggccguc       60

accgacgaga acuacaaccu guacaaguac gccgggaagc gcaugugggg cauccggcug      120

uucgaggcgg ccgacacggc cgccgagcgc cggaugaagc gcuccaaccg gcgccggcuc      180

agccgcaaga agcagcggau cgaccugcug caggagcugu ucuccgagga gaucgugaag      240

aaggaccuga ccuucuucau ccgccugaac gagagccggc uccacuucga ggacaagucc      300

accggggaga aguacccccu guucguggac aaggaguaca ccgacaucga cuaccacaag      360

caguacccga cgauguacca ccuccgcaag gagcucaucg agaaccccga gccccacgac      420

auccggcugg ucuaccuggc cugccaccac auccucaaga accgcggcca cuuccugauc      480

aacggcagca ucuccgacgu gaagaaccug agcuucgugu gcggggagau gaucaaggag      540

uucaacuccg ucagcgagua cgagaucucc cucaccgaca uccgggagcu ggagcgcauc      600

cugagcgaca agaagaccgc gaaguccgug aaggccaagc aggucgccca gcuguucgag      660

ugcacccccg ccgaggacaa ggagcagcag aagugcgcga aggccaucau cacgaaccuc      720

agcaagcuga ucgugggccu caaggggaac aucuccaaga uguuccacca gccgcuggag      780

gagaucagca ccaaguccuu caaguucacc gaggccaagu acgaggacga gaucaugccc      840

gccaucgagg agaacuaccc cgacuacgcc agcgugaucg accggaucaa gggcguguac      900

gacugguccg uccuggugga gauccucgac ggggaggacu accugagcuu cgcgaaggug      960

aaggccuacg agaagcacgg caagaaccug cacauccuca agaacgucau gaagaaguac     1020

ugcaaggacg agguguacca ggccuucuuc aacggcauca acgagaagaa cggguacggc     1080

aacuacaucg gggccauccg caagaacggc cacaccuaca agauggaccg gugcaagagc     1140

gaggaggacu ucuacaaggg ccugcagaag auccugucca cgaucgugcc cgaccaggag     1200

gacgaggagc uccuggagaa gcugaaggcc gaggucgaga accagacccu ccugccgcug     1260

gcgcgcagca aggagaacgg gaccaucccc aaccaggugc accuccagga gcugaugaag     1320

auccuggaca acgccaagga guaccucgac uuccugaaga aggaggacga guacggcacc     1380

aucgccgaga agaucaucuc caucgccaag uuccggaucc ccuacuacgu ggggccccug     1440

agccgccgcc acaaggagga gggcuccaac agcuggauga uccggaagga ggaggggcgc     1500

aucuacccgu ggaacuucgg cgacaagguc gacguggagc aguccaacaa ggaguucauc     1560

gagcggauga cgaacaagug caccuaccuc aucggcgagg acgugcugcc caagaacagc     1620

cugcucuacu ccaaguacau gguccugaac gagcugaaca accugaagau ccgcgggaac     1680

aagaucagcg uggagcucaa gcagcggauc uacaaggacc uguucugcac cauggccaag     1740

gucaccggca agcgcauccu ggaguaccuc aagauggagg accccgagau cgugcaggag     1800

gacaucuccg gguucgacau cgacuucaag agcgcgcugg ccuccuaccu ggacuucaag     1860

aagcaggucg ugggcaacga gaucgagaag gaccacuaca aggagaucau cgaggagauc     1920

aucaagugga agacgaucua cgacgacgac agcaagauga ugcggcgcau gaucgagcgg     1980

gaguaccccg agguguucga caaggagcag aucaagaaga ucugccagcu caaguacucc     2040

ggguggggca acuucagcga ccgcuuccug aacggcgugc gcggggccga caaggagacc     2100

gaggaguccu acaccaucau cgaggcccug uggaagacca acuacaaccu caugcagcug     2160

cugagcaagc aguucuccuu caaggaggag aucgaccaga ucaacgccga gaaggcgggc     2220

gacgucggga agaucaacua cgagaacacg gugaaggacc ucaucgugag cccgagcaac     2280

aagcgggcca ucuggcagac cauccagauc gccgaggaga ucaagaaggu caugaagugc     2340

gagcccgagc ggaucuucau cgagauggcc cgcggcgggg agaaggagaa gaagcggacc     2400

gugucccgca agacccggcu gcaggagcug uacgcgggcu gcaaggacga cacgcgcaac     2460

uggaugaagg agaucggcga ccgggaggag cgcgaguuca acagccgcaa gcucuaccug     2520

uacuacaccc agauggggaa gugcauguac accggcgacg agaucgacgu ggacgcccug     2580

augaagggga acuccaacug ggacaucgac cacaucuacc cccagagccg gaucaaggac     2640

gacucccucg acaacauggu ccuggugcgc aaggacguga acaacaagaa gcggaacaag     2700

auccugagcc ccaagauccg cgaggacaug aagggcuacu ggaccaugcu cugccagaag     2760

ggccugaucu ccaagcggaa guacgaccgc cugacgcgga ccaccgacuu caccgacgag     2820

gagcucagcg gguucaucga gcgccagcug gucgagacgc ggcaguccag caaggccgug     2880

gccgagcugc ucaaccgccu guacaaggac uccgaggugg ucuacgugaa ggccggccug     2940

gugagcgacu uccggcacga caacgaccuc cugaaguccc gccgggucaa cgacuaccac     3000

cacgcgaagg acgccuuccu gaacaucgug guggggaacg ucuaccacgu gcgcuucacc     3060

agcaacccgc ggaaguggau caaggacaac cgcgacucca acuacagcau ccggcgcgug     3120

uucaacuacg acgucaagca gggcgacaag aucguguggc acggguccga gaacggcggc     3180

cacagcaucg accagaucaa gaagaucaug agccggaacg acauccucua caccgaguac     3240

accuacugcg agaaggggca gcuguucaac gagacccugg ccaagaaggg caacgagaag     3300

gccaucgccc ucaagaagaa ccuggacccc gugaaguacg ggggcuacac gacccccaag     3360

accuccgcgu ucgccuucgu cgaguucgac gggaagaaga aggagcgcaa gaaccacauc     3420

guggagaucc ccguguacgu cgugaacauc gccaagcggg agccggacgc cgugaagaag     3480

uaccuggagg agcgcaaggg cuacagcaac gucgugauca agaaguaccc caucaagaag     3540

aacucccuca ucaagaugga cggcuaccug gcccgccugc gcggggcgaa cgagaaggac     3600

aucauccuca agaacgccgu gcagcugauc cugaacaagg accucuacga gaucguccgg     3660

aagaucgaga aguaccugga guacaaccug caguaccagg ccgacgccaa guucgagggc     3720

uucaccauga aggaccucaa caugaucuac gaggagcugu accugaagcu caaggagagc     3780

aucuuccaga agcggcccgc gcagcagaug aagacgcugg agaaggggaa ggaggccuuc     3840

aucuccaucg agaaccuggg cgagaaggcc aaggugauca acgagaucau cgccauguuc     3900

cgcugcgacg ccaccaccgu ggcgaaccuc aaggucauca acgggagcca gaacgccggc     3960

uccaugaagg ugaacaagaa cacccugacg agcaaguccc ugaagcucgu gcaccagagc     4020

aucaccggcc uguucgagac cgaggaggag cuggucuga                            4059


<210>  1877
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1877
augaagaaga aguacuaccu gggccucgac aucgggacca acagcguggg cugggccguc       60

accgacgaga acuacaaccu gugcaaguuc aacgggaaga agaugugggg cauccgccug      120

uucgaggacg ccaagacggc ggccgagcgg cgcaccaagc gguccaaccg ccggcgcauc      180

gcccggacca agcagcgcau cgaccuccug caggagcugu gcgagaaguc caucuucgag      240

aaggacccca ccuucuucau ccggcucaac gagagcagcc ugcaccugga ggacaagucc      300

acgggggaga aguacccgcu guucguggac aaggaguaca ccgacaucga cuaccaccgc      360

gaguacccca ccguguacca ccuccggaag gagcugaucg agaaccccga gccccacgac      420

ccgcgccucg ucuaccuggc cugcugccac aucaucaaga gccggggcca cuuccuggug      480

aacggcucca ucagcgacgc caaggaccuc uccuucaucu gcgagaacau gaucaacagc      540

uucaacgcgg ccuccgacua caagaucaac auccaggaca ugaccgcccu ggagacgauc      600

cugaccgacc gcaagauggc caagagcgug aaggcgaagc agaucgucca gcuguucaag      660

ugcauccccu gcgaggacaa ggagcagcag aagugcugca aggccaucau caccaaccug      720

uccaagcuga ucguggggcu caagggcgac aucagcaaga ucuucaacga guccuucgag      780

gagaucaaga gcuucaaguu cgccgagacc aaguacgagg acgagauccu gcccacgaug      840

gaggagaucu accccgacca cgccgagauc aucgagaaga ucaaguccau cuacgacugg      900

agcauccugg acgagauccu cgacggggag gaguaccugu ccuucgccaa ggugaaggcg      960

uacgagaagc acaagaagaa ccugcacgac cucaagaucc ugaucaagaa guacugcgac     1020

gacgagaccu accagagcuu cuucaacggc augaacgaga agaacaagua cagcaacuac     1080

aucgggucca uccggaagaa cggccacacc uacaaggucg agaagugcaa gagcuccgag     1140

gacuucuaca aggagcugcu caagcugcug agcuccguga acccgggcca ggaggacgag     1200

agccuccugg agcagaucaa guucgagacc gagaaccaga cgcugcuccc ccugcugcgc     1260

accaaggaga acgggaccau ccccaaccag gugcacaugc aggagcucau caagauccug     1320

gacaacgccu ccaaguaccu ggacuuccuc aacgagaagg acgaguacgg caccacggcc     1380

gacaagauca ucagcaucgc caaguucaag auccccuacu acgucgggcc gcuguccgac     1440

cggcacaagg agaacggcag caacuccugg auggugcgca aggagaacgg ccggaucuac     1500

cccuggaacu ucgacgacaa gguggacgcc gagaagucca acaaggaguu cauccagcgc     1560

augaccaaca agugcaccua ccugaucggg gaggacgucc uccccaagaa cagccugcug     1620

uacagccggu acauggugcu caacgagcug aacaaccuga agauccgcgg caacaagauc     1680

uccguggagc ugaagcagca gaucuacaac gaccucuucu gcaccaaggc gcgcgucacg     1740

gggaagaaca uccugaacua ccugaagaag gaggaccccg agcucgccgc cgaggaccug     1800

agcggcuucg acguggacuu caaguccgcc cugaccagcu accucgacuu caagaagcag     1860

gucgugggga acgagaucga gaaggacuac uacaaggaga ucaucgagga gaucaucaag     1920

uggaagacca ucuacaacga ggacuccaag augaugaagc ggaugaucgg ccgcgaguac     1980

ccggagaccc ugaaccagga ccagaucaag aagaucugcc accugaagua caacggcugg     2040

gggaacuuca gcgagacguu ccucaacggc aucaaggggg ccaacaagga gaccggcgag     2100

ugcuacacca ucaucgaggc gcuguggaag accaacuaca accugaugca gcuccugagc     2160

aagcaguucu ccuucaagga ggagaucgac cagaucaacg ccgagaagac gggggccauc     2220

ggcaagauca ccuacgagaa ccuggugaag gaccucgugg ucagccccgc caacaagcgg     2280

gccaucuggc agaccaucca gaucaccgag gagaucaaga aggugaugaa gugcgagccc     2340

gagcgcaucu ucaucgagau ggcgcggggc ggggagaagg agaagaagcg cacggucucc     2400

cggaagaccc gccugcagga gcuguacacc ggcugcaagg acgacacccg ggacuggaug     2460

aaggagaucg gggaccgcga ggagcgggag uucagcuccc gcaagcucua ccuguacuac     2520

acccagaugg gcaagugcau guacacgggg gaggagaucg acguggaccu gcucaugacc     2580

gccaacagca agugggacau cgaccacauc uacccccagu cccggaucaa ggacgacagc     2640

uucgacaaca uggugcuggu ccgcaaggac aucaacaaca acgugaaggg caacaacauc     2700

gugccgucca gcauccagaa ggagaugacc uccuacugga agaugcugcu ccagaaggag     2760

cugaucagca agaagaagua cgagcggcug acccgcucca cggacuucac cgacgaggag     2820

cucagcggcu ucaucgagcg gcagcugguc gagacccgcc aguccagcaa ggccguggcc     2880

gcgcugcuca accggcugua caagaacucc gagaucgugu acgucaaggc cgggcuggug     2940

agcgacuucc gccacgacaa caagcuccuc aagagccgcc ggaucaacga cuaccaccac     3000

gccaaggacg ccuuccugaa caucgugguc ggcaacugcu accgcaccaa guucacggcc     3060

aacccccggc aguggaucaa gaagaaccgc gacaccaacu acuccauccg gcgcguguuc     3120

gacuucgaca ucaagcaggg ggacaagauc gugugguacg gcaacaagaa cgagaacggc     3180

aagcacacca ucgaccagau caagaaggcg augagccgga acgacauccu cuacaccgag     3240

uacacguacu gcgagaaggg gaagcuguuc gacgagaccc ugaccaagaa gggcaacaag     3300

aaggccaucc cccucaagaa gaaccuggac ccccugaagu acgggggcua caccuccguc     3360

aagacgagcg ccuucgccuu caucgaguuc gacgacaaga agaaggagcg caagaaccac     3420

aucguggaga ucccgaucua cguggccaac aucgcgaagc gggagcccga ggccgucauc     3480

aaguaccucg aggagcagaa gggguacgug aacaucgugg ucaagaagua ccccaucaag     3540

aagaacuccc ugcugaagau cgacggcuac cucgcccgcc ugcggggcgg ggacagcucc     3600

aucaucaccc ugaagaacgc cgugcagcuc gugcuggaca agaaguccua cgagaucauc     3660

cgcaaggucg agaaguaccu ggaccacaac agcaaguacg aggccaacag caagcucgag     3720

aaguucaacg agaacgaccu gaacaucauc uacaacgagc ugugccucaa gcugcggaac     3780

uccaucuacc agaagcgccc cgugcaccag cuggagaccc ucgagcaggg caucgacaac     3840

uuccggagcc uggagucccu gaaggacaag gcgaagauca ucaacgagcu ccuggccaug     3900

uuccgcugcg acgccaagac cgggauggac cugcggcuca ucggcggggc cagcaacucc     3960

ggccgcaugc ggaucagcaa gucccgccug acgagcaagu cccugaagcu caucaaccag     4020

agcgugaccg gccuguucga gaccgaggag gagcuggucu ga                        4062


<210>  1878
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1878
auggagaaga acuacuaccu gggccucgac auggggacca gcuccguggg cugggccguc       60

accgacgaga acuacaaccu gaugcgcaag aaggggaagg accugugggg cgugcgccuc      120

uucaaggagg ccaagacgag cgcggagcgg cgggugaagc gcaccucccg gcgccggcug      180

cagcgcgaga aggcccggau cggguaccug aaggagcugu ucgccgacga gaucaacaag      240

gucgacgccg gcuucuacca gcgcauggag gacagcaagu ucaucuccga cgacaagacc      300

gugcaccaga aguucacccu guucagcgac acgaacuaca ccgacgagga cuacuacaac      360

gacuacccca ccaucuucca ccuccggaag aagcucaucg aguccgacga cgaguacgac      420

gugcgcaugg ucuuccuggc ccugcucaac auguacaagc accggggcca cuuccugaac      480

gcgaaccuga gcgacaacgg gaugggcgac aucaacgagc aguacgaccg ccucaaggag      540

cugugcuccg aguaccugaa cguggagugg gagaacaagc cggacaaguu cgaggagauc      600

cucaaggagu gcaacugcag caacucccug cggaacgagc gccugcucga gaccuucaac      660

cugaagaaga cggacaagga gaagugcgag augcugaagc ucaucugcgg gcugagcggc      720

acccugucca agaucuucaa ggaccucgac gacgacagcg agaccaagaa gaugaccauc      780

uccuuccgga gcuccaacua cgacgaggag gccaucaacg uggaggagau caucgggagc      840

gagaacuucg agaucaucgc cguccugaag gagauccacg acugguccau gcuggccaac      900

aucaugcgcg gcaagaagua ccucagcgug gcgcgggugg aggaguacga gaagcacaag      960

gacgaccuga agauccugaa gaaggucauc aaggaguaca gcaucgagga guacaacaac     1020

uucuuccgcg ugauggccga caacaacuac uccgccuacg ugaacagcgu caacuccaac     1080

aaggagagca cgcggcgcgg cgccaagugc gacaacgaca ccuucuucaa ggagaucaag     1140

aagaucguga acaagugcua cgacuccgag gagaaggagu acauccucaa ggagaucgag     1200

aaggggaccu uccugcccaa gcagaucauc agcuccaacg gcgugauccc caaccagguc     1260

cacaagaucg agaugaagaa gauccugcag aacgccgaga aguaccuccc cuuccugacc     1320

gagaaggacg acagcgggcu guccgugagc gagcgcaucc uccagauguu cuccuuccag     1380

aucccguacu acaucggccc caucagcggc aagaucgggu ccgacgagaa guccaagaac     1440

agcuggagcg ugcggaagga gaagggccgc aucuaccccu ggaacuucaa ggacaagauc     1500

gacgagaagg aguccgcgga gaaguucauc gagcggaugg ucaaccacug cacguaccug     1560

aacaacgaga uggugcugcc caagaacagc cuccuguacg agaaguucau ggugcugaac     1620

gagcucaaca accugaagau caacaacgag aacaucuccg ucgagcugaa gcagagccuc     1680

uacaacaacc uguucaagac cgggaagaag gugaccgcca agggcaucaa gagcuaccug     1740

gagcgcaacg ggcugugcga gccgaacgag gacaucaacc ucaccggcau cgacggcgac     1800

uucacgaacc ggcuguccaa cuacgccaag uucaagggga ucuucaacac cgacgagcug     1860

accuacgagc aguggaacau ggccgagaag aucaucaagu ggagcaccau cuacggcgac     1920

uccaagaagu uccucgagga gaagauccgc aacgaguaca gcgagaagcu ggacucccag     1980

cagaucaagc ggauccuggg gaucaaguuc cgcgacuggg gccggcucag cgaggagcug     2040

cugaacaugg agggggccga cgucaacacg ggcgagauca aguccgugau cggccgcaug     2100

ugggacgaga acuacaaccu cauggagcug aucgcgaccg acaacuucac cuacaagcag     2160

gucaucaagg agaagcagaa cgggcuggac aagagccucu ccgagaucga cuacgacgac     2220

cuggacgagc uguacgugag cgcccccgug aagcggauga ucuggcagac ccuccugauc     2280

aucaaggagc ugguguccgu caucggcuac gagcccacgc gcguguucgu ggagauggcc     2340

aaggacguca acaccgagaa gaagcggacc gacagccgca agaagaaguu ccucgaccug     2400

uacaagcagu gcgagaagga cgagcgggac uucuggauca aggagaucga cgacaagucc     2460

gaggccgagu uccgcaagaa gaagcuguac cucuacuaca cccagaaggg gcggugcaug     2520

uacacgggcg agcagaucaa cccccuggac cugaccaacg acaaccucua cgacaucgac     2580

cacaucuacc cgcgccacuu cgugaaggac gacagcaucg agaacaaccu ggugcugguc     2640

aagaaggaga agaacgccca caaguccgac accuucccca ucgaggacga cauccggaag     2700

aaccaguacg cguucuggaa gaugcucuac gagaagaacu ucaucaccaa ggugaaguac     2760

cagcgccuga cgcgcaagga gcccuucagc gacgaggaga aggccaacuu caucaugcgg     2820

cagaucgugg agacccgcca ggggaccaag gucaucaccg acauccugga gaaguccuuc     2880

gacaagaccc agaucgugua cgugaaggcc ggcaacguca gccucuuccg gcagaaguac     2940

gagugcauca agugccgcga gaucaacgac uuccaccacg ccaacgacgc cuaccugaac     3000

aucguggugg gcaacgucua cgacacgaag uucaccagcg acccccggaa cuucaucaag     3060

gaguaccgca agaacccgca gaagaacgag uaccacaugu acaagcuguu cgacuacaac     3120

gugcagcgcg gggaccgggu ggcguggacc gccuccaacg gcgagagcau gaaggucgug     3180

aaggggaccc ucaagaagaa cacgccccug gugaccuuca uguccuacga ggagcacggc     3240

ggccuguacg accagaacau caucagcgcc aaggacaucg agaaggucaa ggggaagggc     3300

uaccucccca ucaaguccag cgacucccgg cugaacaaca ccgagaagua cgggggcuac     3360

gccaagguga gcggggcgua cuucuucgug gucgagcaca ccgugaagaa cgugcgcauc     3420

cggacgaucg agcccaugcc gugcuaccug accaaggucc ucaccaccaa ggagaagauc     3480

gaggacuacu gcugcaacau ccugaaguac gacucccccg uggugaagua cgagaagauc     3540

aagauguaca gccugaucaa ggucaacggc uaccccaugu accucacggg ccgcaccggg     3600

aaccagcuga ucguguccaa cggcauccag cuggugcuca gcuacgacga ggccaaguac     3660

aucaaggacc ugcugaagau caaccaggag gaguucgacc agaaccggcu cgagaaccug     3720

gggaucaaca aguuccgcaa cgagaagcug uacgacaccc ucaacaagaa guacaccaac     3780

gagaucuaca agaagcggcc caacuccaug ggcaacaaga ugaucgagug gaagaacacg     3840

uuccagaacc ugagcaucaa ggaccagacc gagguccugc uccagcugau ccagcugggg     3900

cagaagcaga acuccggcgu gaaccucgag aagcugggcg ccagcaagaa gaccggggug     3960

ucccugguca gcaagaagau caccgacaag gaguccuucg agcucaucaa caagagcauc     4020

acgggccugu acgagaacaa gguggaccug cuccgcgugu ga                        4062


<210>  1879
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1879
auggccgccu ucaaguacua ccugggccuc gacaugggga ccaacagcgu gggcugggcg       60

gucaccgacc ccaacuacaa ccugcugaag gccaagggga aggaccucug gggcauccgc      120

gaguucaacg aggccuccac ggccguggag cggcgcaccc accggaucag ccgccggcgc      180

cggcagcgcg agcagguccg gaucgggcug cugaaggacu acuuccacga cgccaucggc      240

gagguggacc cggacuucuu ccagcgccuc gcgaacucca aguaccaccu ggaggacaag      300

gacaccgagg ugcgguacaa gaacaacgug uucaacgacg acgacuacac cgacaaggac      360

uacuucgacc aguaccccac gaucuaccac cuccgcaagg agcugaucga gagcaccgac      420

aagcacgacg uccggcuggu guuccuggcc cuccugaaca uguucaagca ccgcggccac      480

uuccugaacu ccgggcucgg cgagaacagc ggggagaaca acaucaacaa cgccuaccug      540

gagcugacca accuccuguc cgaguucacc caguacaacc ugaacgagac gaucgacggc      600

aagaagaucg aggacauccu cagccggcgc gacauguccc ggacccgcaa ggccgagggg      660

cuggcggaga uccugggcgu ggacuacaag aacaagcccu acaaggagcu cauccggggc      720

cugugcgggc ugaaguucaa cgccugcgcc aucuuccccg agauccagag cgacgagguc      780

ccgaagcucg acaucugccu guccgaggcc agcuucgacg agaaguccga cgagaucgcc      840

aacauccugg gcgaggacua cuucgagauc aucauggcga ugaaggacau cuacgacauc      900

gggagccucg ccggcaucau gaagggccac aacuaccugu cccaggcccg cgugaccagc      960

uacgagaagc acaaggagga ccugcggcuc cugaagaacg ugauccgcaa guacugcucc     1020

aaggaggacu acgacaacuu cuucaacagc gaggccgacg gguccuacgc cagcuacguc     1080

ggcagcuaca acuccaagac caagcagcgc cgggugggga acaagcgcag cuccgaggag     1140

cuguacaagg cgaucaagaa gauccucaag aacgugcccg ccgaggacgc cgacguccag     1200

cggauccuga ugagcaucga gaacgagacg uuccugccca agcagcucac cgccuccaac     1260

ggcgugaucc ccaaccaggu gcacgccaag gagaucaaga agauccugag caacgcggag     1320

cgcuaccugc cguuccuccg ggagaaggac gagaccgggc uguccgucag cgagaagauc     1380

augaagcugu ucuccuucca gauccccuac uacgugggcc ccaccacgga gaaguccagc     1440

aaggacggcg ggaacggcug ggugguccgc aaggaggagg gccaggugcu ccccuggaac     1500

aucgacgaga agaucgacau gaaggccacc agcgaggccu ucaucucccg gauggugcgc     1560

cggugcaccu acaucagcgg ggagccgguc cugcccaagu ccagccugga guaccagucc     1620

uucugcgugc ucaacgagau caacaacauc aagaucgacg gggagcgcau cagcgucgag     1680

cugaagcagg acaucuacaa ggagguguuc cagaagggca agcgggugac caagaagcag     1740

cugugcaagu accugcacac gcgcggcauc aucgaggccg aggagcaggu caccgggauc     1800

gacgugacca ucaacaacgc ccucaccacg uacggcaagu ucaaggcgau ccugggggac     1860

gacaucucca aggacagcgu gcagaagaug gucgagaaca ucguguucug gugcaccgug     1920

uacggcgaca gcaagaaguu ccugaaggac cggaucgagg agaaguacgg ggagaagcuc     1980

accaaggagc agaucaagcg caucgucggc uucaaguuca aggacugggg caaccugucc     2040

aagaacuucc uggagcucag cggggccgac guguccaccg gcgagagcgu guccaucauc     2100

cgggcccugu ggaacaacaa ccugaaccuc auggagcuga ucaacagccg ccuguacaac     2160

uacaaggagc ggcucgccga guaccagaac acgaugauga agacccuguc cgacaucgag     2220

gcggaggacc uggacgagua cuacuucagc gcccccguca agcgcaugau cuggcagacc     2280

auccucauca ucaaggagcu ggugaaggug cuggggugcg agcccgaccg gaucuucguc     2340

gagaugaccc gccgcccgga cgagcggaag augcgcacgg agucccggcg caagaaguuc     2400

gaggagcucu acaagaaggu gaaggacgag gacguggacu ggaugaaggu caucgcccac     2460

gccgacgaga ccggcagcau ccgguccaag aagauguacc uguaccugac ccagaagggc     2520

cgcugcaugu acaccgggaa gcacaucgag cucagcgacc uguucaacaa caaccuguac     2580

gacaucgacc acguguaccc ccggcacuuc gugaaggacg acaacaucga caacaaccuc     2640

guccuggugu gcaaggagaa gaacgcccac aaguccgaca ucuacccccu ggaggacgag     2700

auccucgcga gccagaagaa cauguggucc gagcugcgcc ggagcggcuu caucacggag     2760

gagaaguaca accgccugau cgggcggaac uccuucaccg acgagcagaa ggccggcuuc     2820

aucgcccgcc agcucgugga gaccagccag gggaccaagg gcgucgccaa cauccugcag     2880

cagcugcucc ccgagagcaa gaucguguac gccaaggcgu ccaacgugag cgaguuccgg     2940

aacacccgcg acaucccgaa gucccggcug aucaacgagu uccaccacgc ccacgacgcc     3000

uaccugaaca ucgucguggg caacguguac uacgucaagu ucacgcagaa cccccucaac     3060

uucaucaaga acgacuacga ccgcgacaag accaagaaca acuacaaccu gagcaagaug     3120

uucgacuggg acguggagcg gaacggggag guggccugga ucgcccagaa gaaggacggc     3180

gaggcgggga ccaucgccac cgucaagaag gugcugggcc gcaacacgcc ccucaugacc     3240

cgguacuccu ucgaggggaa gggcggccug accaacgaga cccuguacag cgccaagaag     3300

gccaaggagg agggguacau ccccuucaag uccagcgacg agaagaugaa ggacgugacg     3360

aaguacggcg gguucaccuc cgucaccggc gcguacuucu uccucgugga gcacaccgag     3420

aagaagaagc gcagccggau gaucgagucc gugccgcugu accuggccca gaagaucgag     3480

cagaaccccc acgagcucga gaaguacugc gagcaggucc uggggcugau cgacuucgac     3540

auccgcgugc gcaagaucaa gaucggcagc cucaucaagc ggaagggcua cuucuucuac     3600

aucacgggga agaccggcca ccgccugauc cugcggaacg ccgugaacau gugccucaag     3660

aaggaguggg ucaaguacau cuccaagcug gagaaggccg uggacaagaa caacguggag     3720

gggaucauca ccaaggagaa gaacgagcug cucuacagcg agcugaucga gaaguucaag     3780

aacuccaucu ucagcaaccg ccccaacccc gucggcgaca agcuggacga ggccaaggag     3840

aaguucauca accucgacau cgagaagcag ugcaucgugc ugugccagau ccugcagcuc     3900

uccagcgugc ugggguccga gugcgacaug acccugcucg gcgagagcgc gcacugcggc     3960

aagauccuga ucagcaagaa gaucggggac gaggacaucu accugaucaa ccaguccguc     4020

acgggccucu acgagaagaa gaucaaccug cggaccgugu ga                        4062


<210>  1880
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1880
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cuucaucaac       60

gaggccgaga acgagaucga gguguccagc aucgucaaca ccggggugcg caucgugccc      120

cugacgaccg acgaggaguc cgacuucaag aagggcaaca ccaucagcau caacgcggac      180

cggacccuca agcgcggggc ccggcgcggc cugcagcggu ucaagcagcg ccgggaggcc      240

cugcucgaga cguucaaccg caucaaguuc aucgccaccg acuucgucua cgccgagacc      300

ggcgcggcca ccacguucuc cagcuaccag cugcgggcca aguccgccgu ggaggcggug      360

accaaggagg agcuggucaa ggugcuccug augcugaaca agaagcgcgg guacaagagc      420

ucccggaagg ccaagaccgc cgaggagggc gacgccaucg acgggaugaa gaucgccaag      480

gagaucuucg agaacaaccu caccccgggc cagugggugu acaacagccu guccaagggc      540

gggaaguuca uccccgacuu cuaccacagc gaccugcaga aggagcucga gaagaucguc      600

cgcuuccaga accaguucca cuccgagcag aucaacgaca agcugcuggc ggacauccag      660

ggcaagagcc ggacgcagac cucccagcac uucagcaaga cccucaacau cccccuggcc      720

gagaacaagg ggacccgcga ggagacgaag cugcagcacu accaguggcg gaacgaggcc      780

cucgccaacg agcuggacau gaagauccug gccuucauca ucaccgagau caacaaccag      840

aucaaccagu ccagcggcua ccucggggcg aucuccgacc gcagcaagga gcuguacuuc      900

aaccaggaga ccgugggcca guaccaguac aagcagcugc aggagaacgu gcacgccaag      960

cucaagaacc aggucuucua ccggcaggac uaccuggacg aguucgagaa gaucugggag     1020

acccaggcca aguuccacag cgagcugaac gagaagcuca aggccgagau ccgcgacauc     1080

acgaucuucu accagcggaa gcugaagucc cagaagcacc uggugagcuu cugcgaguuc     1140

gagaagggcc acaaggccau ccccaagucc agcccgcugu uccaggaguu ccgcaucugg     1200

cagaaccuga acaacguggu cgugaagaac gagaccacca aggagguguu cgagcugucc     1260

gaggagcuca agcagcugau cgcggaggag cugcaguuca aggagagcau gaccgacguc     1320

cagaugcuca cguucugcga gcugaagaag gggacccaca ccgugaacuu caagaagauc     1380

gagggcaacc ggaccaacac guccaucuac aaggccuucg agaagauccu ggagguggag     1440

ggguacgaga ucgacuucag caagaugaag ccccucgaga ucaaggagac cgucgccccc     1500

aucuucgaga cccugaacau caacaccgug auccuggacu ucgaccccac gauccagggc     1560

aacgacuucg acaagcagcc guacuaccag cucuggcacc uccuguacuc cgccgaggac     1620

gacgacaagc ucaaggagac ccugaagacc aaguucgggu ucaaggagaa ccaccucccc     1680

uucauccuga acaucaaccu gcaggccgac uacggcagcc uguccgcgaa ggccaucaag     1740

aagauccugc cccaccucau ggacggccac aucuacgaca aggccugccu gauggugggg     1800

uacaaccaca gcuccagccu gaccacggcc cagaacaacg accgcguccu caaggacacc     1860

cuggagcugc ucaagaagaa cucccugcgc aaccccgugg ucgagaagau ccugaaccag     1920

augaucaacg ugaucaacgc gaucaugaag gacccggcca ugggcaagcc cgacgagauc     1980

cggguggagc ucgcccgcga gcugaaggcc aacaacgagc agcggaagaa ggccaccagc     2040

gacaucaaca ccgcgaccac ggagcacgag cgcauccgga agcugcucca cuccgaguuc     2100

aacauccccc gcgucacccg gaacgacauc auccgcuaca agcuguggcu ggagaccggg     2160

ggcaucagcc ucuacaccgg gaaccccauc aaggccuccg accuguucag caagaacuac     2220

gacaucgagc acaucauccc caagagccgg cuguucgacg acuccuucag caacaagacg     2280

cucugcgagc gccagcugaa caucgacaag uccaacaaga ccgccuacag cuuccugcag     2340

gagaagcucu ccgagaacga cuucgccgcc uacgagaacc gggugaagga ccuguucaag     2400

accggcaaga ucaagagcua caccaaguac aagaagcugc ucauggcgga caacgagauc     2460

ccggagggcu ucaucgaccg ccagcugcgg gagacgcagu acaucgccaa gaaggccaag     2520

gagauccugc ucgagguguc ccgcaacguc accgccacca ucgggagcgu gaccgacaag     2580

cugcggcagg acugggagcu gguggacguc augaaggagc ucaacuggga gaaguacgac     2640

aagcugggcc ugacgcacau cgaggagggg aagaacggcg agcgccucuc caagaucaag     2700

gacuggacca agcggaacga ccaccgccac cacgccaugg acgcgaucac cguggccuuc     2760

accaagcccg ccuacaucca guaccugaac aaccugaacg ccaagacgca gggggagaac     2820

aaggcgaaca gcaucaucgg caucgagaac aagcaccucu accgggacaa gaacaacaag     2880

cugcgcuuca ucucccccau gaagaacuuc cgcgaggagg ccaagaagca gcuggagagc     2940

auccucaucu ccuacaaggc caagaacaag guggucacca agaacaagaa caccaccaag     3000

aaguccggcg ggacgaacca gaagauccag cugaccccgc ggggccgccu gcacaaggag     3060

accguguacg ggaagcucca gcaguacgcc accaaggagg agaaggugaa cgccagcuuc     3120

acggaggacu acauccagaa ggucgcgaag aaggaguacc gggaggcccu gcugaagcgc     3180

cucgaggaga acgacaacga ccccaagaag gccuucaccg gcaagaacgc ccugaacaag     3240

acccccaucu acaucagccu gaaggacaac aucaucgugc ccgagaaggu gaagaccguc     3300

uggcucgaga cggacuacac cauccggaag gacaucaccc cggaccugaa gaucgacaag     3360

gugaucgacg ugggcaucaa gcgcauccug cagaaccggc ucaacgaguu caacggggac     3420

cccaagaagg ccuucgucaa ccugggcgag ucccccaucu ggcugaacaa ggagaagggg     3480

aucgcgauca agcgcgugac caucagcggc gugagcaacg cccaggcccu ccacacgaag     3540

aaggaccacc uggggaacga gauccuggac aagaacggca accccauccc ggucgacuuc     3600

aucuccaccg gcaacaacca ccacguggcc aucuaccggg acgagaaggg gaaccuccag     3660

gaggaggugg ucagcuucua cgacgccgug auccgccgga accugggccu guccgugauc     3720

aacaagaacc acgagaaggg gugggaguuc cucuucagca ugaagcagaa cgaguucuuc     3780

aucuuccccu ccgacggcuu caacccccac gagaucgacc ugcugaaccc gaacaacuac     3840

caccucauca gccccaacau cuuccgcguc cagaagaucu ccaccaagaa cuacauguuc     3900

aaccaccacc uggagaccaa ggcgguggac ggggagaugc ucaagagcaa gaaggagcug     3960

uccaagacga gcuaccacuu cauccagacc cccaccaacc ugaacggcau caucaaggug     4020

cggaucaacc accugggcaa gaucguccac cucggggagu acuga                     4065


<210>  1881
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1881
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cuucaucaac       60

gaggccgaga acgagaucga gguguccagc aucgucaaca ccggggugcg caucgugccc      120

cugacgaccg acgaggaguc cgacuucaag aagggcaaca ccaucagcau caacgcggac      180

cggacccuca agcgcggggc ccggcgcggc cugcagcggu ucaagcagcg ccgggaggcc      240

cugcucgaga cguucaaccg caucaaguuc aucgccaccg acuucgucua cgccgagacc      300

ggcgcggcca ccacguucuc cagcuaccag cugcgggcca aguccgccgu ggaggcggug      360

accaaggagg agcuggucaa ggugcuccug augcugaaca agaagcgcgg guacaagagc      420

ucccggaagg ccaagaccgc cgaggagggc gacgccaucg acgggaugaa gaucgccaag      480

gagaucuucg agaacaaccu caccccgggc cagugggugu acaacagccu guccaagggc      540

gggaaguuca uccccgacuu cuaccacagc gaccugcaga aggagcucga gaagaucguc      600

cgcuuccaga accaguucca cuccgagcag aucaacgaca agcugcuggc ggacauccag      660

ggcaagagcc ggacgcagac cucccagcac uucagcaaga cccucaacau cccccuggcc      720

gagaacaagg ggacccgcga ggagacgaag cugcagcacu accaguggcg gaacgaggcc      780

cucgccaacg agcuggacau gaagauccug gccuucauca ucaccgagau caacaaccag      840

aucaaccagu ccagcggcua ccucggggcg aucuccgacc gcagcaagga gcuguacuuc      900

aaccaggaga ccgugggcca guaccaguac aagcagcugc aggagaacgu gcacgccaag      960

cucaagaacc aggucuucua ccggcaggac uaccuggacg aguucgagaa gaucugggag     1020

acccaggcca aguuccacag cgagcugaac gagaagcuca aggccgagau ccgcgacauc     1080

acgaucuucu accagcggaa gcugaagucc cagaagcacc uggugagcuu cugcgaguuc     1140

gagaagggcc acaaggccau ccccaagucc agcccgcugu uccaggaguu ccgcaucugg     1200

cagaaccuga acaacguggu cgugaagaac gagaccacca aggagguguu cgagcugucc     1260

gaggagcuca agcagcugau cgcggaggag cugcaguuca aggagagcau gaccgacguc     1320

cagaugcuca cguucugcga gcugaagaag gggacccaca ccgugaacuu caagaagauc     1380

gagggcaacc ggaccaacac gugcaucuac aaggccuucg agaagauccu ggagguggag     1440

ggguacgaga uggacuucuc caagaugaag ccccucgaga ucaaggagac cgucgccccc     1500

aucuucgaga cccugaacau caacaccgug auccuggacu ucgaccccag cauccagggc     1560

aacgacuucg acaagcagcc guacuaccag cucuggcacc uccuguacuc cgccgaggac     1620

gacgacaagc ucaaggagac gcugaagacc aaguucgggu ucaaggagaa ccaccucccc     1680

uuccugcuca acaucaaccu gcaggccgac uacggcagcc uguccgcgaa ggccaucaag     1740

aagauccucc cccaccugau ggacggccac aucuacgaca aggccugccu gauggugggg     1800

uacaaccaca gcuccagccu caccaccgcc cagaacaacg accgcguccu gaaggacacg     1860

cuggagcucc ugaagaagaa cucccugcgc aaccccgugg ucgagaagau ccucaaccag     1920

augaucaacg ugaucaacgc gaucaugaag gacccggcca ugggcaagcc cgacgagauc     1980

cggguggagc uggcccgcga gcugaaggcc aacaacgagc agcggaagaa ggccaccagc     2040

gacaucaaca ccgcgaccac cgagcacgag cgcauccgga agcuccugca cuccgaguuc     2100

aacauccccc gcgucacgcg gaacgacauc auccgcuaca agcuguggcu cgagaccggg     2160

ggcaucagcc uguacaccgg gaaccccauc aagggcuccg accuguucag caaggacuac     2220

gacaucgagc acaucauccc caagagccgg cuguucgacg acuccuucag caacaagacc     2280

cugugcgagc gccagcugaa caucgacaag uccaacaaga cggccuacag cuuccuccag     2340

gagaagcugu ccgagaacga cuucgccgcc uacgagaacc gggugaagga ccuguucaag     2400

accggcaaga ucaagagcua caccaaguac aagaagcucc ugauggccga caacgagauc     2460

ccggaggggu ucaucgaccg ccagcugcgg gagacccagu acaucgcgaa gaaggccaag     2520

gagauccucc uggagguguc ccgcaacguc acggccacca ucggcagcgu gaccgacaag     2580

cugcgggagg acugggagcu cguggacguc augaaggagc ugaacuggga gaaguacgac     2640

aagcuggggc ucacccacau cgaggagggc aagaacgggg agcgccuguc caagaucaag     2700

gacuggacga agcggaacga ccaccgccac cacgccaugg acgccaucac cguggcguuc     2760

accaagcccg ccuacaucca guaccugaac aaccucaacg ccaagaccca gggcgagaac     2820

aaggccaaca gcaucaucgg caucgagaac aagcaccugu accgggacaa gaacaacaag     2880

cugcgcuuca ucucccccau gaagaacuuc cgcgaggagg cgaagaagca gcucgagagc     2940

auccugaucu ccuacaaggc caagaacaag guggucacga agaacaagaa caccaccaag     3000

aaguccgggg gcaccaacca gaagauccag cugacgccgc gggggcgccu ccacaaggag     3060

accguguacg gcaagcugca gcaguacgcc accaaggagg agaaggugaa cgccagcuuc     3120

accgaggacu acauccagaa ggucgccaag aaggaguacc gggaggcgcu gcucaagcgc     3180

cuggaggaga acgacaacga ccccaagaag gccuucacgg gcaagaacgc ccugaacaag     3240

acccccaucu acaucagccu caaggacaac aucaucgugc ccgagaaggu gaagaccguc     3300

uggcuggaga ccgacuacac gauccggaag gacaucaccc cggaccugaa gaucgacaag     3360

gugaucgacg uggggaucaa gcgcauccuc cagaaccggc ugaacgaguu caacggcgac     3420

cccaagaagg ccuucgucaa ccugggggag ucccccaucu ggcucaacaa ggagaagggc     3480

aucgccauca agcgcgugac caucagcggg gugagcaacg cgcaggcccu gcacaccaag     3540

aaggaccacc ugggcaacga gauccucgac aagaacggca accccauccc ggucgacuuc     3600

aucuccacgg ggaacaacca ccacguggcc aucuaccggg acgagaaggg caaccugcag     3660

gaggaggugg ucagcuucua cgacgccgug auccgccgga accuggagcu cuccgugauc     3720

aacaagaacc acgagaaggg gugggaguuc cuguucagca ugaagcagaa cgaguucuuc     3780

aucuuccccu ccgacggcuu caacccccac gagaucgacc ugcucaaccc gaacaacuac     3840

caccugauca gccccaacau cuuccgcguc cagaagaucu ccaccaagaa cuacauguuc     3900

aaccaccacc uggagaccaa ggccguggac ggggagaugc ucaagagcaa gaaggagcug     3960

uccaagacca gcuaccacuu cauccagacg cccaccaacc ugaacggcau caucaaggug     4020

cggaucaacc accucggcaa gaucguccac cugggggagu acuga                     4065


<210>  1882
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1882
auggccgacu ucgccaagua ccagcccggc augguggcgg acgggcgcaa gaucccgcug       60

gacuggaccc ucuacuaccu gcggaagaag gcccugaccc aggccauccg caaggaggag      120

cucgccugga uccugcugaa cuucaaccag aagcggggcu acuaccagcu ccgcggggag      180

gaggaggagg agaacagcac gaagcacgag gaguacuacg agcugaaggu cguguccgug      240

gaggccgcgg aggagggcaa gggcgggaac accugguaca acgucacccu ggagaacggc      300

uggaucuacc ggcgccagag caagaucgag cucaacgacu ggguggggaa ggugcggccc      360

uucaucguca ccacggagua cgagaucgac ggcgugacca ucaagaagga caagaacggg      420

gaggugaagc gcuccuuccg ggcccccagc gagaacgacu ggggccugcg caagaagcgg      480

accgaguccc ugcucgagga gagcgggaag accgucggcg ccuucaucua caaccacauc      540

cuguccgagc ccgccgacaa gauccgcggc aacuucaucc ggacgaucga gcgcaaguuc      600

uacaagaagg agcuggaggc gauccuccgg gagcagagca agcaccagaa ggagcugaag      660

gacaucgaca cccugaucgc cugcaucaag gagcucuacc cgaacaacaa gccccaccag      720

gacucccuga ucaagaagga caugaccuac cugcucauca ccgaccugau cuucuaccag      780

cgcccccuca agagcaagaa gucccucauc gccgacugcc ccuacgagua cuacgaguac      840

gugaucaagg agacggggga gaacaagcgg cagagcauca agugcaucgc caaguccaac      900

ccguacuacc aggaguuccg ccuguggcag uucaucauca accugcggcu guucgaccgc      960

aacaacgaca aggaggugac cagcgaguac cuguccagca uggaggacua cacccggcug     1020

uucaccuacc ucaacgaccg caaggacauc aaccaggaga cgcugcugaa ggacuucuuc     1080

aagcagaaga aggucaagcg cggcauggag aaggaguucc ccauccggug gaacuacauc     1140

gaggacaagg agaagaagua ccccugcaac gagacccgcc acgagcuccu gaccgcccug     1200

gggaaggugg gcaucgacca cuccuggcuc gacgacaagg agcuguucua ccggcugugg     1260

caccuccugu acagcgugga guccaaggag gaggcggagg gcgcccugcg caagcucguc     1320

gacaacgacg acuucgugaa cgccuuccug aagaucaagc ccuucaagaa ggaguacggg     1380

gccuacagcg agaaggccau caagaagcug cucgcgguga ugcggauggg caaccugugg     1440

aacgaggucg acaucugcga gcagacccgc gaccggaucc agaacaucau caagggggac     1500

aucgacgaga agaucaagga ccgcaugggc cggacguuca gccagcuguc cgacuuccag     1560

gggcucccgg aguggcuggc cugcuacgug guguacggcc agcacagcga ggccaccgac     1620

auccagcgcu ggaccaagcc cgagaaccug auggccuaca ucaacggcuu caagcagcac     1680

ucccugcgga accccaucgu cgagcagugc auccucgaga cccugcgcac ggugcacgac     1740

aucuggaagg aggccgggca caucgacgag auccacaucg agcugggccg ggggaugaag     1800

uccaccgcgg accagcgcgc ccggaugagc aagagcauca ugcagaacga gaacaccaac     1860

cuccgcauca agucccugcu gauggagcuc aagaacgcca aggaguucga gaacgugcgg     1920

cccuacagcc cgaugcagca ggagauccug cgcaucuacg aggagggcgc ccugcaggag     1980

cucaccaagg aggacgccca guacaacgag aucuccaaga ucagccagau ggcgcagccc     2040

uccgccaacg agcugcugaa guaccggcuc uggcuggagc agaaguacug cagccccuac     2100

acggggaagc ccaucucccu gagcaagcug uucaccuccg ccuaccagau cgagcacauc     2160

aucccgcaga gccgcuacuu cgacgacucc uucaccaaca aggucaucug cgaggccgag     2220

gugaaccagc ucaagagcaa caugcugggc uacgaguuca ucaagaagca cggcggggag     2280

aucguccgcu gcaccaugau cggcgacgug aagcuccugg acgcgaagga guacuacaac     2340

uucgugacgg agcacuacac caacaaccgg cgcaagaagg agaagcugcu cauggaggac     2400

aucccccagg aguuccugaa ccggcagaug aacgacuccc gcuacaucag caagaagguc     2460

auggccaucc ugagcaacau cgugcgggag gaggacgagc aggaggccaa guccaaguuc     2520

gugaucgccu gcagcggggg caucaccgac cgccucaagc aggacugggg gcugaacgac     2580

gucuggaacu ccaucgugua cccccgguuc gagcgccuga acaagcucac caacacggag     2640

aacuucggcc ggugggagaa caagaagggc aagcaggucu uccagaccag cgugccccug     2700

gagcugcaga aggacuucuc caagaagcgc aucgaccacc ggcaccacgc cauggacgcg     2760

cucgugaucg ccugcgccag caacaacauc aucaacuacc ugaacaacga guccgccaag     2820

agccaggaga agcgcgagga ccugcggaag aagcucugcg acaagaaccg caucauccgg     2880

aagccguggg agaccuucac ccaggacgcc cacaccgcgc uggagaacgu ggucgugucc     2940

uucaagaacu acauccgcgu gaucaacaag gccacgaacu acuacgagaa guacgacaag     3000

aacgggaaga aggagaucgc cgagcagaag ggccaggaca ugugggccau ccggaagccc     3060

augcacaagg agaccgucuu cggccaggug aaccugcgcc ggaagaucac caucaagcuc     3120

aaggacgccc uggagaagau ccccgcgauc ugcgacaagg agcugcgcga cuacaucaac     3180

auccucgugg ccaagcaguu caacaagaag cagauccugg cccacuucaa gagcaucaac     3240

uaccggcuga acaugaaguc cgucgacaag guggacgugu ggcaguucag caacgagaag     3300

gagcccaugg ucgccacccg caaguccgug gacacgagcu ucgacaccaa gcgcaucgcg     3360

accaucaccg acacggggau ccagaagauc cuccugaacu accugaaggc caagaacaac     3420

gacccgaaca ucgccuucac ccccgagggc aucgccgaga ugaacaagca caucaccgag     3480

uacaacaacg ggaagcagca ccagcccauc cucaaggugc gggucuccga gccgaagggc     3540

gccaaguacc agguggggga gaccggcaac aagcgcagca aguucgugga ggcgcagaag     3600

gggacgaacc uguacuucgc caucuacgag gacaaggagg gcaaccgguc cuacaacacc     3660

aucccccuga acgaggucau cgagcgccuc aagcagggcc ugagccccgu gcccgacgcc     3720

aacgagaagu ccaucccgcu gaaguuccac cucagcccca acgaccuggu guacgucccc     3780

aagaccgacg aggagaacga gaucaagaug gggcagaucu acaaguucgu ggacagcucc     3840

ggcaccacgg ccaacuucgu gccccaccgg gucagcaacc ugaucuacaa cguggacaag     3900

aagaucgccg agaacuucug caaccacgag aucauccaga acgaguacgg gaucggcucc     3960

ccgcagagca agaaccagaa guccaucacc ggggagauga ucaaggcggu gugcuggaag     4020

cucgaggucg accgccuggg caacauccac aagaucauca aguga                     4065


<210>  1883
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1883
augaagaaga uccugggccu cgaccugggg accaccagca ucggcugggc cuucaucaag       60

gagcccgaga aggacguggu cggguccgag aucguggaca ugggcgugcg caucgucccg      120

cugagcuccg acgaggagaa cgacuucgcc aaggggaaca cgaucagcau caacgcggac      180

cggacccuca agcgcggcgc ccggcgcaac cugcagcggu ucaagcagcg ccggaacgcc      240

cugcucgaga ccuucaagga gaagaaccug auccccucca acuuccagua cgccgaggac      300

ggccccagcu ccaccuucag cacgcugaag cuccgcgaca aggccgcgaa ggagaagauc      360

gagcuggacg acuucgugaa ggugcugcuc cagaucaaca agaagcgggg guacaagucc      420

agccgcaagg ccauguccga ggaggacgag ggcaccgcca ucgacagcau ggggaucgcc      480

aaggagcugu acgagaacga ccugaccccc ggccaguggg ucuacgaggc gcucaagaac      540

gggcggaaga acgugccgga cuucuaccgc uccgaccugc aggaggaguu caagaagauc      600

gugaacuacc agagcgaguu cuaccccgac auccugaaca ccuccuucgu cgaggacugg      660

augggcaagg ccagcacgcc caccaagcag uacuucaaca agcggggcgu gcagcucgcc      720

gagaacaagg ggaagcgcga ggagcggcgc cugcaggagu acaaguggcg ggccgaggcc      780

gugaacuuca agaucgaccu guccgagauc gcgcucaucc ugagccagau caacucccag      840

aucagcaaca gcuccggcua ccugggggcc aucagcgacc gcuccaagga gcucuacuuc      900

aagaaccuga ccgucggcca guaccuguac cagcagguca agaagaaccc ccacacccgg      960

cucaagggcc agguguucua ccgccaggac uaccuggacg aguucgagaa gaucuggagc     1020

gugcagucca gcuucuaccc gcagcugaac gacgagcuca agcaggaggu gcgggacgug     1080

acgaucuucu uccagcgccg ccugaagucc cagaagcacc ugaucagcaa cugcgaguuc     1140

gaggagcacc acaaggucgu gcccaagucc caccccaucu uccaggaguu ccggaucugg     1200

cagaaccuca acaaccugac ccugaucaag aaggacaacc ucaacgagaa gugcgagcug     1260

gcccuggagg acaagaccgc ccucgccaac gagcuggcgu ucaagaagga gcugagcgug     1320

aaggacgccc ucaagguccu ggggcugaag cccuccgacu gggaguucaa cuucaccaag     1380

aucgagggca accgcacgaa ccacgccuuc uucgaggccu ucgccaagau caucgagcuc     1440

gaggacgggg agccgaucga ccugggcaac cugaaggcgg acgacauccu cgaccaguuc     1500

agcgaggccu uccugcggau cgggaucgac accgagcugc uccaggugaa cuccgacauc     1560

gagggcgccg aguacgagaa gcagagcuac auccaguucu ggcaccuccu guacuccagc     1620

gaggacgacc agaagcugaa gcagaaccuc auccgcaagu ucggcuucaa gcccgagcac     1680

gccaagaucc uggccuccau cagccugcag gacgaccacg cgucccucag cagccgggcc     1740

aucaagaaga uccugcccca ccuccagucc gggcugaucu acgacaaggc cugcaccuac     1800

gccggcuaca accacagcuc cagcuucacc aaggacgaga acgagaagcg cgagcugcgg     1860

gcggagcugg agcuccugaa gaagaacucc cugcgcaacc ccguggucga gaagauccuc     1920

aaccagauga ucaacguggu caacgccauc cugaaggacc cgaaccuggg gcggcccgac     1980

gagauccgcg uggagauggc ccgggagcuc aaggccaacg ccgagcagcg caagaacaug     2040

acgagcaaca ucgcguccgc cacccgggac cacgagaagu accgcgagau ccugaagaag     2100

gaguucggcc ugaagcgggu gaccaagaac gaccuccugc gcuacaagcu guggcucgag     2160

accgacggga ucagccugua cacgggcaag cccaucgagg ccuccaagcu guucagcaag     2220

gaguacgaca ucgagcacau cauccccaag gcccggcugu ucgacgacuc cuucagcaac     2280

aagaccaucu gcgagcgcca gcugaacauc gacaaggcca acgucaccgc guucuccuuc     2340

cugcagaaca agcucagcgc cgacgaguuc gagcaguacc agucccgggu caagagccug     2400

uacggcaagc uguccaaggc caagauccag aagcuccuga uggccaacga caagaucccg     2460

gaggacuuca ucgcccgcca gcugcaggag acccgcuaca ucagcaagaa ggcgaaggag     2520

auccucuucg agaucacgau gcgggugagc gugaccaccg ggaccaucac ggacaagcug     2580

cgcgaggacu ggggccuggu ggagaucaug aaggagcuca acugggagaa guacgacaag     2640

cuggggcuga ccuacaccau cgagggcaag cacggggagc ggcucaacaa gaucaaggac     2700

uggaacaagc gcaacgacca ccggcaccac gccauggacg cccugaccgu ggcccugacg     2760

aagcccgcgu acauccagua ccucaacaac cugaacgcca aggccaucgg caacaagaag     2820

ggcaccgaga ucuucgccau cgagcagaag uaccugaagc gcgagaacgg gaagcucugc     2880

uucaucccgc ccaucgagaa cauccgggcc gaggcgaaga agcaccuguc caagauccug     2940

gucagcuaca aggccaagaa caagguggug accaucaaca agaacaagac caagaagaag     3000

gccggcuuca acgagcagac cgcccucacg ccccgcgggc agcugcacaa ggagaccguc     3060

uacggcaagu ccuuccacua cagcaccaag uucgagaaga ucggcgccuc cuucgacgcg     3120

cagaagauca acaccguggc caagaaggag gagcgggagg cccugcucaa gcgccuggcc     3180

gagaacggga acgaccccaa gaaggccuuc acgggcaaga acagccugaa caagaugccc     3240

aucuaccucg accuggggaa gaacaucaag cuguccgaga aggugaagac cgucgugcuc     3300

gagcagaacu acaccauccg gaagaacauc gaccccgacc ugaaggugga caaggucauc     3360

gacgugggca ucaagcgcau ccuggagagc cggcucgagg aguucggggg caacgcgaag     3420

cuggccuucu ccaaccugga ggagaacccg aucuggcuca acaaggagaa gggcaucccc     3480

aucaagcgcg ugaagaucag cggggucucc aacguggaga gccugcacgu gaagaaggac     3540

cacuucggcg agaagauccu ggacgaggag gggaacgaga uccccgucga cuucgugucc     3600

accggcaaca accaccacgu ggccaucuac gaggacgagg acgggaaccu ccaggacgag     3660

gucgugaacu ucuacgaggc cgugauccgg cgcaaccagg gcgagagcau caucaaguac     3720

cggcacgagg agggcuggaa cuuccuguac uccaucaagc agaacgaguu cuucgucuuc     3780

ccgagccagg acuucaaccc caacgacuuc gaccugcucg accccaagaa cuacgaccug     3840

aucuccccca accuguaccg cgcgcagaag uucagcaagg ugauguacgg gaacuccgcc     3900

gugcgggacu acgucuuccg ccaccaccuc gagacggugc ugaacgacgu gaaggagcug     3960

aaggacgucg ccuacaagca gcucaagagc cugcaguacc ugaacggcau caagaaggug     4020

cggcucaacc accuggggca gaucauccag gugggcgagu acuga                     4065


<210>  1884
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1884
augacccugc acuacaacgu gggccucgac aucgggacca acagcgucgg cuacacgauc       60

guggacgaca acgggaagau ccuccaggug aagggcaaga acggguacgg cguccgcgug      120

uucaaggagg gcgccaccgc cgcggagcgg cgcaucuucc ggaccaccca ccgccggcug      180

aagcgccgga aguggcgccu ccggcugcug caggacuucu ucgagcccua cauccucccg      240

caggacgacg gguucuucau ccgccggaag gaguccaacc uggugcugaa cgaccgcgac      300

gaggacacgg ccagccucuu caacgaccgg uccgaccgcg acuucuacca ggccuacccc      360

accaucuacc accuccggca ggcccugaug accgagaagc gccaguucga cguccgggag      420

aucuaccugg ccaugcacca caucaucaag uaccgcggcc acuuccugcu gaacgggacc      480

gugaacaacu ucacgaacaa caagaucgac cuccacagcg acuucgagga gcugaacgcg      540

cugcuccuga acgugauccc cgagcugaag uuccagaucg accucgacca cuccaccaug      600

uuccagacca cccugcugaa cacgaccauc agccgguccg gccgccagcg ggaccucguc      660

aagagcuucu acaacggguc cgacgacaag cagcuggcca agcagcagaa guccguggcc      720

accgagcugc ucaaggccau ccugggccug aaggcgaagu uccacaagcu guucaaccug      780

gaccugcucg agaccacgga gugggaguuc agcuucgacg ccgaggacau cgacgacaag      840

cuggccaagc uggagccccu caugaccgag aaccagcagc aggucaugga cauccugcag      900

cgcaucuucg ccgccaucac ccugaacggc aucgugccgg aggggaagac ccucagcgcg      960

uccaaggugg acagcuacca gaagcacaag caggaccuga agcugcucaa ggccaucgcc     1020

cagcacuccg acaagaagac ggccaccgug cugcagaccg ccuacaacga guacaucaac     1080

ggcaucgcga gcaagccccu gaagcaggag gacuucuaca agcgccucac caacgccguc     1140

aagaagucca gcgaccccca gguggccgac augcuggccc ugaucgacca ggagaaguuc     1200

cuccccaagc agcggacgaa ggagaacggg gccaucccgc accagcugca ccagcuggag     1260

cuggagcgca ucaucgacaa ccagaaggac uacuaccccu ggcucgcgga gcugaacccc     1320

aaccaggccc ggcagaaggu ggccaaguac aagcuguccg agcucgucgc cuuccgcgug     1380

cccuacuacg ugggcccgcu gaucgagccc gagguccagc aggccaccag caacgcgcac     1440

uucgccugga uggcccggaa ggaggccggc cccaucaccc ccuggaacuu cgaccagaag     1500

guggaccgca acgugagcgc ggagcgguuc aucaagcgca ugaccacgaa ggacaccuac     1560

cugcucgccg aggacguccu gccgcugcac ucccuccugu accagcgguu caucgugcug     1620

aacgagcuca acaacguccg cgugaacggg cagaagcuga ccaagaagca gaagcaggcc     1680

guguaccagg accuguucaa gcggcagccc cacgucacca agaagcaguu caagagcuac     1740

cucgugcaga cgggcgaguu cgccgagucc agcaagaucg aggggcuggc caacgagacc     1800

uccuucaaca gcggccugac caccgagaac gagcuccgca agaucuucgg ggcgcagcug     1860

gacgaggccc gguaccaggu ggacuucgag cagaucaucg agugggccac gcuguucgag     1920

gacgccaaga uccuccgcac caagcuggcc gagaucaccu ggcugaccac cgagcagauc     1980

gagaagcucg cgggcauccg guaccgcggc ugggggcggu ucucccgcaa gcugcuggcc     2040

ggccuccggg accagaacgg gcagcagauc aucgaccugc ugugggacac gcccaacaac     2100

uucaugguca ucgugagcca ggccgccuuc uccgaggcca ucaccaagga gaacgagaag     2160

cucaucgacc gccgcggcgc gcaggacgug aucaccgacc uguacaccag cccccagaac     2220

aagaaggccc ugcggcaggu ccucgccauc guggccgacg ugcagaaggc gaugggcggg     2280

gucccgcccc agcgcaucuu caucgaguuc gcccgggagg acgagaagaa cccccgccgg     2340

uccguggagc gcagccggca gcuggagaag cuguaccaga cgaucuccaa cgaguuccuc     2400

aucaacagcg aggugcgcca ggagcugaag gaggccgucg accagaaggu gaacuucaag     2460

gaccggcugu uccucuacuu ccugcagggc gggguggacc uguacuccgg caagcgcauc     2520

aacaucgacc agcucagcca cuacgacauc gaccacaucc ugccccaguc cuucaucaag     2580

gacgacagcc uggacaaccg gguccucgug ucccagaagc ugaaccgcag caagagcgac     2640

uccgugccgc ugagcgaguu cgccaacuac aaguucgggc ccgccaugca ggcgaagugg     2700

cuccagcuga aggaggccgg ccugcucucc aagcggaagu acgacaaccu gacccuggac     2760

cccaccaagc ucagcaaguu cgcgccccug ggguucguca accgccagcu gguggagacc     2820

cgccagguga ucaagcucgc cgccaaccug cuggccuccg aggagacgaa ggucgugacc     2880

aucaaggcca acaugaccca ccaggugcgg aaggagcucg acuucccgaa gaaccggaac     2940

gucaacaacu accaccacgc cuucgacgcc uaccugaccg ccuucguggg caucuuccug     3000

agcaagcgcu accccaagcu caagcccuac uucacguacg gcgacuucca gaaggggaac     3060

aagcugccgg accugaagaa cuucaacuuc cucuacgagc ugaagaacaa ggaccggucc     3120

aucgacagca acaccggcga gaucaucugg gacaagcagc gcgaccuggc guacaugaac     3180

aagaucuaca acuucaagaa ggugaccguc gugcacgagg ugcucaccaa guccggggcc     3240

cuguacaacc agacgcugua caaggccagc gaggacaagg ccuccggccg gggcaccaag     3300

cagcucaucc gcaagaagga caacaugccc accgagcugu acgggggcua caccgggagc     3360

acguccgcgu ucaugagcau cguccgccug uggaagaagg acaagcccua cuacaaggug     3420

gugggcaucc ccacccggau ggccgccaag cucgccaacc aggcccagcu gcuggacuac     3480

cucaccaaga aguucaccac gcgcaagcug gucaagaaga ccggggacua caagaccacc     3540

guggagcggu ucgagcuggu ggucccgaag gugggcuuca accagcucgu gaucgacggc     3600

gggcagcccu ucaugcuggg cuccgcgacg uaccaguaca acgcccgcga gcuguucauc     3660

agcaaggagg ccgucaaggc ccucaacaag cagcugucca cccacgugga ccuggugcag     3720

gucuucgacg agauccucgu ccaggugaac cggugguucc cccuguacga caccaacggg     3780

uuccgcgaga agcugagcca cggccgggac cgcuucauga agcucaacga cagcuucgag     3840

gagaaguccg acacccagau cgacgugcug aaccggaucc ugaucggguu ccacgccaac     3900

gcggcccgca cgaaccucaa gauccugggc cugggcaccg accugggguu caugacccag     3960

caggccggca uccggcucac cgagaaggcc auccuggugc accagagccc cuccgggcug     4020

uucgagcgca agguggcccu ccgggaccug aagggccugc gcuga                     4065


<210>  1885
<211>  4068
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1885
augaccuaca gcaucggccu ggacaucggg accggcuccg uggggugggc cgucaucggc       60

gacgacuacc gccucaagcg ggccaagggg aagaaccuga ucggcgugcg ccuguucgac      120

agcgcgacga ccgccgccga gcggcgcggc uaccggacca cccgccggcg ccucucccgg      180

cgccacuggc ggcugcgccu gcucaacgac aucuucgcca gcgaccuggc cgagcaggac      240

gagaacuucc ugccccggcu caaguacucc ugggugaacc cggacgacga gcagaacccc      300

cagcugaaca gcgacgucgc gaacggggcc cuguucggcu ccagcgagac ggacaaggcc      360

uuccacaaga acuaccccac caucuaccac cuccgccacg agcugaugac cgacaccgcc      420

aagcacgacc ugcgggaggu guaccucgcg auccaccaca ucgugaagua ccgcgggaac      480

uuccugaaca cggccgacaa gaucaacgcc gagaacuccu ucaacgucga ccuguucauc      540

gccgcccucg acgaguacaa cgaguuccug gagaccccca ucaccagcau caaggacgcg      600

cagcuguucu ccgacaagcu caccgacauc aacgcccgga acaagagcgu gcgcguggag      660

aacgccaugu ccgagacgua cgacgacaag agcaagaucu ucaaggccau ccugaccgcc      720

cuggucggca acaccgcgaa cuuccagacc aucuucgacc ucuccgaccu ggagaaggac      780

gaggccaaga acuucaaguu caagcuggac cucgaggacg uggagacgaa gcuggacgug      840

guccgggcca agcugagcga cgagcagaac gccuuccucg aggccgugcu gaucgcguac      900

gacgggauca cccugaagau gauccucggc gacuacaagu ccaucagcca guccaugauc      960

gagagcuaca agagccacca gcgcgacugg aaguacauca agaacaacgu gcggcugaac     1020

gagcagaaga ucgagccgga caagaacucc gucaaguucu ucaagcugga ccccaagaag     1080

cucaucuacc ccagcaaccu ggccuaccuc caggugaucu ccagcaacga ggacgagcac     1140

cgcaagggcc uggacuacuu caagaccgcc auccaggacg ccgacauccu gcagcgcauc     1200

gagaacgaca ccuuccugcc ccggcagcgc acgaagcgga acggggugcu cccguaccag     1260

cugcaccugg ccgagcucga gcagaucauc aagaagcagu ccaaguacua ccccuuccug     1320

gcgaacaucu acacccagga caacaagacc gagaacaagc ugaucggccu ccugaaguuc     1380

cgcguccccu acuacguggg gccccuggug gaggccgaga aggucaccgg cgacggcaag     1440

aaccacuggc ugaucaagaa ggagcuccag gagaacacgc cgaucacccc cuucaacuuc     1500

gacgacgugg uggacaagga caagagcggg gagcaguuca ucaagcggau caccggcacc     1560

gacuccuacc ugaucgggga gcccacgcug cccgagaaca gccuccugua ccagaaguac     1620

aacguccugc aggagcucaa caacgugcgc aacgagcggc accagcgccu gccgaucaag     1680

gucaagcagg acaucuucga gcacguguuc aagaucgaca agaacgugaa cgccaagaag     1740

gucauggccu accugaagcu caacggcuac ggggacuucc agaucgcggg ccuguccgac     1800

acccggaccg gcaaguucaa cagcaaccug uccagcuaca acuacuucgu ggaccuccug     1860

gggcgcgaga agaccgagaa gauccgggag gaccgccugg aggagaucau cgagauccag     1920

acgguguucg aggacaagau ccucgucgcc aagcggcugu ccaaccugga cuuccucacg     1980

ccccaggaga aguccaagcu gagcgccaag cacuacaccg gcugggggcg ccugagccag     2040

aagcuccuga ccacccaguu caucaagacc gcccuguccg aggccggcga caucaucccc     2100

cagagccacu ccaucaucga ccuccuguac uacaccgaca agaaccugau ggagauccuc     2160

aacgacaaga ccuucaacgu gcggaacugg cugaccgagc agaacaaggg gacggagagc     2220

ggcaacagcc uguacgacca gaucgacgcg cucgccggcc ccaaggacau caagcgcggg     2280

aucacccagg uguucuccau caucgacgac aucaagaagg ccaugggcag caacccggag     2340

accgucuacc uggaguucgc ccgggagacc cagacguccc gccugaccaa cagccggcuc     2400

aaccgcgugc aggagaucua caacuccccc gagcugaaga ccgaguucgc cuaccuggcg     2460

aagaagcucg aggccgagac caaggagagc auccacgacg accgccugua ccuguacuac     2520

cuccaggagg ggcgggacau guacacgggc aaggagaucc ccuucgacca gaucuccagc     2580

aacuacgaca ucgaccacau caucccccag gccuucacca aggacgacuc ccuggacaac     2640

cgcgugcugg ucaaccgggc caccaacgcc cgcaagagca acuccccgac cuucacgaac     2700

gacaucaucg acaaccgcaa gagcuggugg cagucccucg ugaagcgggg gcugaugagc     2760

aaggagaagu ucgagcggcu gaccgacaag cgcaagaccu ucuccgagaa ccagaccaac     2820

cacuucaucg cgcggcagcu cguggagacg cgccagauca ucaagaacgu cgccgaccug     2880

aucaagaaca acuacgacgg cacccaggcc guggccaucc gggcgagccu gaccggcgag     2940

augcgccggu accucgacug gcccaagaac cgcgacauga acgacuacca ccacgcccac     3000

gacgcccuga ugauggccac cguggggaag uacgucgacc ggcgcggcuu cuucgacaac     3060

ggguccguga gcgacggcgc cggcaacgcg uacaaccugu acacgaagga cuggcucgug     3120

gccgcccggg ucgggacccg caacgacagc cagcggguga accccuacgg cuucaucgug     3180

ggguccauga acagcaaggc cguccgccag caggucaaca aggagaccgg cgagaucgug     3240

ugggggaacg agaaccugga cuaccugcgg cacgugcucu ccuacaagaa gauccuggug     3300

acccgccgcc ugagcggcuc cagcggccgg cucuacgccg agacgcuguu cucccccaac     3360

ccgaaggcga agcugaucgc ccagaaggcc aaccgcagcg uggcccucua cgggggcuuc     3420

accaaggccg aguccgcgua caccgcccug gucgaguuca acaagaaggg ggaccggagc     3480

uacaagcuga ucaaggugcc cauggccauc gccaaccgcg ugaacgacaa gcagaucacc     3540

gucacggcgu acaucgccuc ccucaaccug cgggacuuca gccgcguggu gcuggagcac     3600

cucccccugg gcacccugau ccgggagucc gacgggagcg aguucuacgc cgccuccagc     3660

gaguacaagc acaacgccaa ggagcucugg gcgccgaagg agauccugcg cuccagcaag     3720

aagcuggaca aggccaccgc cgacaaccuc aucgacaucu ucgacuuccu gaccaacgac     3780

aagacgaucu ccaacuucaa guucuacgag aaggaccugc uccaccugaa gaacguccgg     3840

gacaaguucg uggaggccag cgacgagauc aagaucaagg ugcugagcga cgucaucuac     3900

gagcuccaca acgacgccgg cuggcgcgac cccuucaagc acauccagga gaagcccgcg     3960

uggaccaugc uccaggugaa gggcgggauc aagcuguccg agaaggccgc caucguguac     4020

cagagcccca ccggccuguu cgagaccauc acgaucgucg gggaguga                  4068


<210>  1886
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1886
augaagaagu ucgaggagua cuacaucggc cuggacaugg ggaccggcag cguggggugg       60

gccgugaccg acuccgccua caacaucauc aagcgccacg gcaaggcgcu cugggggauc      120

cggcuguucg agagcgccaa gacggccgag gagcgccgga ucuuccgcac cucccggcgc      180

cggaccgagc gccggaagaa ccgccugcac cuccugcagg agaucuucgc cgaggagauc      240

agcaagaagg acaugggcuu cuaccagcgg augaaggagu ccaaguacug gcacgaggac      300

aagaaggaca ucaacggcaa caugcccgag cugccguacg cccucuucgc ggacgacggg      360

uucaccgaca aggacuacua caagaaguac cccacgaucu uccaccuccg cuacgcccug      420

gccaccaaca ccaccgacaa gcccgacauc cggcugcugu accuggcccu ccaccacauc      480

aucaagcacc gcggccacuu ccuguucgag gggaagaagc ugagcgaggu gaaggaguuc      540

uccacggcga ucgaggcccu cgugcagcag gccgaggagc aggagaucga ccucaagagc      600

cugacccagg aggagguccu gacccagcug gagcacauca uguccgacgc ccagugcacc      660

aagagcgaca agaagaccca gguguccaag cugaucgugg gcacggacaa gcagaagcag      720

aagcaggaga aggccaucgc ggcccucauc accgggugca ccgucaagcu gagcgacauc      780

uucgccgacc ccgagcugga caaggaggag aucuccaaga ucugcuucag cgacaacggc      840

uacgaggaga aggaggugga gaucgagucc gugcucgccg accgguuccu gcugaucgcc      900

gcggccaagg gcgucuacga cuggacggug cuccacgaca uccuggggga cgcccgcuuc      960

cugagcgagg ccaaggugaa gaccuacgag aagcaccggg acgaccuccg caaggucaag     1020

cagcugcugc ggauggacaa ggagguguac gaggccgugu ucggcgucuc cgagaagaac     1080

gaggcgaacu acagcgccua caucgggaug gccaagaaga acggcaagaa gcagccgauc     1140

gagaagaagu gcucccagga ggacuucuac aaguuccuca agaagcagcu ggaccgccug     1200

agcguggacg agaugggcca ggagaucauc gacgagcuca aggccgagau ggaccugggg     1260

acccuguucc ccaagcagau gauccgggac aacggcguga uccccuacca gcuccacgag     1320

aacgagcuga acgccauccu ggacaacgcg cagaaguucu accccuuccu ccuggagaag     1380

gaccaggacg gguacacccc ggucgagaag auccagaaga ucuuccgcuu ccgcaucccc     1440

uacuacgugg gcccccugaa cacggcccac caggacaagg gcgggcacag cugguccgug     1500

cggaagaagg agggcaagau cuaccccugg aacuucagcg agaugaucga cgaggaggag     1560

uccgccacca aguucaucga gcgcaugacc aacaagugca ccuaccucau cggggaggac     1620

guccugccga aggagagccu gcucuacgcc aaguucaacg ugcugaacga gcugaacaac     1680

cugcggaucg acggcgagcu caucgcgccc gagcugaagc aggacaucua caacaacgug     1740

uucaagaagu accagcgcgu cacggggaag aaggugaagg acuaccugcg gaucaacggc     1800

gucaucucca aggagcagga gcucagcggg uucgaccaga acuucaaguc cagccugaag     1860

gccuaccacg acuucaagca gaucgugggc accggcgugc uguccgagag cgagcaggag     1920

gacaucgucc gcgacaucac ccucuucgcc gacucccaga agaugcugaa gcagcggauc     1980

caccgcaagu accccaugcu gagcgacaag cagaucgccc agcucgccaa caagaaguac     2040

gcgggguggg gccggcuguc ccgcaccuuc cuggagaaca ucgagagcgu gaacaaggag     2100

acgggggagg ugaugaacau caucaccgcc cucugggaga ccaacgagaa ccugaugaag     2160

cugcucuccg gcaaguacga cuacaccaag cagaucgagc aguggaacgc ccggcagaug     2220

aagaaggggg agaucgagua ccaggacauc gaggagcugu acgucagccc cgccgugaag     2280

cgcccgaucu ggcagacgau caagaucgug cgggagauca ccaacaucau gggcggcgcc     2340

cccaagcgca ucuucgucga gguggcgcgg gagcccgggg agaagggcgu gcgcaagguc     2400

ucccggcgca accagcugcu cgaccuguac aaggccugca agaaggaggc ccccgagcug     2460

uacgaccggc ucgccaacaa ggagagcgac aacgcccugc gcuccgacaa gcuguuccuc     2520

uacuacagcc agauggggcg cugcauguac agcggcgacg cgaucgaccu ggacgagcug     2580

uucaccaacc gguacgacau cgaccacauc uacccgcagu ccaaggugau ggacgacagc     2640

aucgacaacc gcgugcucgu caagcgggac cugaacucca agaagaccga cacguucccc     2700

gugagcgagg aguaccgcaa ggccggcaag gcccuguggu ccacccuccu gaagaagggg     2760

cugaucagca aggagaagua caagcggcuc acccgcaccg agcccuucgg cgaggacgag     2820

cuggccgggu ucaucgcgcg gcagcuggug gagacccgcc aguccacgaa ggccgucgcc     2880

caccuccugg aggaggccua ccccgacgcc gagaucgugu acgcgaaggc ccgggccgug     2940

agcgaguucc gccacaaguu cgacaugauc aagguccggg aggugaacga cuuccaccac     3000

gccaaggacg ccuaccugaa caucgugguc ggcaacaccu acuacgugaa guucaccaag     3060

gacgcggccu gguucgugcg caacaacccg gggcggaccu acaaccucaa gaagauguuc     3120

gagaacgaga cggucaagcg cggcaacgag accgccugga uccccggcga caacgggacc     3180

aucugcaccg ugaagaagug gaugcggaag aacaacgugc uguucacgcg ccgggccuac     3240

gaggucaagg gcgggcuguu cgaccagaac auccucaaga agggcaaggg gcagaucccc     3300

aucaagggcg gcgccgacga gcgccugcgg ucccgcgaca aguacggggc guacaacaag     3360

gccagcggcg ccuacuucau gcugguggag uccgacggga agaagggcga gcggaagcgc     3420

accaucgagu ucgugcccgu cuaccucgcc aagagccugg aggaguccgu ggagcugcgc     3480

cggcgcuacu gcgaggagga gcucgggcug gugaacccgg acguccggcu gagcaagauc     3540

cgcaagaacg cgcucaucga cguggacggc uucaagaugc accugguggg ccggaccgag     3600

aaccgccugc uccugaucgg ggccacccag cugguccucu ccgaggacga ccagcagcug     3660

aucaagaaga ucggcaagua cugccuggac caccgggaga acaaggacgc cgugcucagc     3720

gagaaguacg agcugacgau ggaccuguuc gagaagcucu acgacaucuu cguguucaag     3780

cuggagcaga ccgucuacgc cgugaagcug uccaagcagg ccaaggaccu caaggaggug     3840

aaggaggagu uccuggcgcu ggacgacgag cagaagugcc uccugcugau ggagauccuc     3900

caccuguucc agugcagccc ccagcuggcc aaccucacca acaucggggg cggcaagagc     3960

gccggguccc ugaagauccu gagcgacauc accaagcagc aguccguccu ccugaucaac     4020

cagagcauca cgggcuucua cgagcagguc aucgaccuga agaccaucug a              4071


<210>  1887
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1887
auggaggugg gcaacuacua ccuggggauc gacgccggca ccgacagcgu cgggugggcc       60

gugaccgaca accaguacaa cgugauccgc aagaagggca agucccucug ggggauccgg      120

cuguucgacg gcgcgaacac ggcccaggag cgccggaccu uccgcgcccu gcggcgccgg      180

aacgagcgca agaagcagcg gaucaaccuc cugcaggagc uguucugcaa ggagaucugc      240

aagcucgacc ccaacuucuu cauccgccug cgggacagcu cccuguggag cgaggacaag      300

gaggagaagc agaucuacuc ccucuucaac gacaagaacu ucaacgaccg caacuaccac      360

aacaacuaca agaccaucua ccaccuccgg gccgaccuga ucaagaacaa ggagaagcac      420

gacgcccgcc uggucuaccu ggcgauccac cacaucauga agaaccgggg ccacuuccug      480

uucaacggga acaucgagag cgugaccucc uucgagaaca cguucaacga guucaagaac      540

ugccuccugg acgagcugga gaucaaccuc gagugcccga gcaucuccga guucgagaac      600

auccugaagg accgcaccuu caagggcacc cagaagugca agaagcugaa ggagcucugc      660

agcauccccu ccagcgacaa gaagcuggac aacaucuuca agcugaucuc cgggcucaag      720

gccgaccuga gcaucaucuu cgaggacgag uccuucaagg agaacgagca caacaagauc      780

agcuucgccg acuccaacua cgaggagacc cgggaggugc ugaacagcga gauccaggag      840

aagacgcuca ucaucgacau cuucaacucc aucuacaacu gggucauccu gagcgacauc      900

cugagcggcg gggaguacaa cggcggccuc uaccugucca ucgccaaggu gaacaccuac      960

aacaagcaca gcaaggaccu gaagauccuc aaguccguga ucaagaagua cuucccccag     1020

aaguacaccg acaucuucgc gagcaacaag gagaacaacu acugcuccua caucgggaug     1080

uacaagagcg gcgggaagaa guacuccguc aagaagugca gcgacaagcc cgaggaguuc     1140

uacaagacca ucaagaacau caucaacaag aacacggccg aggagaucuc caacgacaag     1200

gacgugcagu acauccugag cgagaucgag aacaacuccu uccugccgcu ccagaucaac     1260

aagaacaacg gcgugauccc cuaccaggcc aacaagaucg agcuggagag cauccugaag     1320

aacgccgaga acuaccuccc cuuccugaag gagaucgaca ccgagugcaa caagaccacc     1380

uccgagaaga ucaucgaccu guucgaguuc cgcauccccu acuacgucgg cccgcucaac     1440

acgagcaagg gggagaacgc cuggaugauc cggaaggagg acggcaccau ccgccccugg     1500

aacuucgagc agaaggugga caucgacaag accgcggccg aguucaucac ccggaugacg     1560

aaccagugca ccuaccugaa caacgagacc gugcugccca aguacucccu ccuguacagc     1620

gaguacaccg uccugaacga gaucaacaac auccgcauca acggggagaa gaucuccgug     1680

gagcucaagc aggacaucuu caacauccug uucaagaaca acaagcaggu cacgugcaag     1740

aagcugcuga gccaccucaa cuccaacggc uacaaccuga agaaggacga ccugagcggg     1800

uucgacggca acuucaagag cucccucagc uccuacaucg accugaagaa cuacaucuuc     1860

ggcgacaaca ucgacaagcc ccagaucuac aaccuggccg agaagaucau ccucuggauc     1920

acccuguacg gggacgacag caagcugcuc aagcgcgugc ugcagaagaa cugcuccgag     1980

cagcugaacc cggagcagau caagaagauc accaagcuca aguucaaggg cugggggcgg     2040

cugagcaagg ccuuccuguc cgagcuccgc ggcgccgacc gggagaccgg ggagacgaug     2100

accaucauca agggccugcg caacacccag aacaaccuga ugcagcuccu gagccagaac     2160

uacaccuuca cggaggagug cgagaagauc aacuccggcu acuucaucaa ccccaacgag     2220

aucagcuacg acuccaucgu gaagaacauc guggcgagcc ccuccaucaa gcggagcguc     2280

uggcagacca uccagaucgu ggaggagauc cagaagguga ugggguccaa gccccgcaag     2340

aucuucgucg agauggcccg gggcgaggag gagaaggugc gcaccaccag ccggaagcag     2400

aagcugcucg accuguacaa gaacaucucc gacgagugcg ccaaggggau guacgacgag     2460

aucuacagca aggacgagag cgacuucaag aacauccgcc uguaccucua cuacacgcag     2520

augggcugcu gcauguacuc cgggaagcac aucgaccuga gccagcuguc caacaccaac     2580

aucugggacc gggaccacau cuacccgcag agcaagacca aggacgacuc ccucgacaac     2640

cugguccugg uguacaagag caucaacucc gagaaguccg acgacaucau cagccccacc     2700

auccagaaca acaugcgcac cuucuggaag cuccuguucg accggaaguu caucagcgag     2760

aagaaguacc accgccugac gcggaccacg ccccucaccg acgaggagcu ggccggcuuc     2820

aucaaccgcc agcuggugga gacccggcag uccaccaagg ucauggccag caucuucaag     2880

cgcaucuacc ccaacaccga gaucaucuac gugaaggcga agaacguguc cgacuuccgg     2940

caccgcaagg acugcgagaa cggcgucaag gugcgcgagc ucaacgacua ccaccacgcc     3000

aaggacgccu accugaacau cguggucggg aacguguaca gcuccaaguu caccaacaac     3060

ccgcugaugu ggcucaagaa caaccggaac gugaaguaca gccugaaccg cauguacgac     3120

uucaaccuga cggucaacaa cgagaacauc uggguggugg gcaacgaggg gacccagaag     3180

accgucaaca aguaccucgg caagaacgac auccuguaca cccgguacgc caugacgaac     3240

aagggcgagc uguucgacca gaacccccuc aaggccaccg gggacaaguc caagcagacc     3300

acccuggugc cccugaagaa gggcaaggag guguggaagu acgggggcua caacagcauc     3360

acgcccuccu acuucaugcu cgucgagagc aaggacaaga aggggaacua cauccgcacc     3420

aucgagacca ugccgcugua ccgggagaag gaguucagca agaacgagga guuccugcuc     3480

aacuacugca aggaggugca caagcugaag gagcccaaga ucauccugaa gugcaucaag     3540

aagaacgcgc uccugaucgu ggacggcuuc cccaugcacc ugaagggcuc caccgggcuc     3600

cagcugaagc ugcagggcgc cguccagcuc auccuggaga gcgacauggu gaucuaccug     3660

aagaagcuca ugaaguucgu gaacgagaac aacaucaaca agaacaagcu ggaggucacg     3720

cccuacaugg agcuguccgc caaggagaac aucaagcucu acgacaucuu cauccagaag     3780

cugaccaccg ggaucuacag caagcgcccg gccaaccccg uggagaagcu gaucaacgcg     3840

cgggacaucu ucaucgagcu caccgugaag gagcagugca ucguccuguc cgagauccug     3900

caccucuucc agugcaagcc ccugacgagc gccaaccugg uguccaucaa ccucaagaag     3960

accgugggca ucauccagau cgccaagaac aucaccgcca agaacgucaa gcuggugcac     4020

cagagcccga ccgggaucua cgaguacacg guggaccuga acaagaucug a              4071


<210>  1888
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1888
augaccaaga aggagcagcc cuacaacauc ggccuggaca ucgggaccag cuccgugggc       60

ugggccguca cgaacgacaa cuacgaccuc cugaacauca agaagaagaa ccuguggggc      120

gugcgccucu ucgaggaggc ccagaccgcg aaggagaccc ggcugaaccg cagcacccgg      180

cgccgguacc gccggcgcaa gaaccggauc aacuggcuga acgagaucuu cuccgaggag      240

cucgccaaga cggacccgag cuuccugauc cgccugcaga acuccugggu gagcaagaag      300

gaccccgacc ggaagcgcga caaguacaac cucuucaucg acgggcccua caccgacaag      360

gaguacuacc gggaguuccc caccaucuuc caccuccgca aggagcugau ccugaacaag      420

gacaaggccg acauccggcu gaucuaccug gcccuccaca acauccugaa guaccgcggg      480

aacuucaccu acgagcacca gaaguucaac aucuccaacc ugaacaacaa ccucagcaag      540

gagcugaucg agcugaacca gcagcucauc aaguacgaca ucuccuuccc ggacgacugc      600

gacuggaacc acaucagcga cauccugauc ggccggggca acgccacgca gaaguccagc      660

aacauccuga aggacuucac ccucgacaag gagaccaaga agcugcugaa ggaggucauc      720

aaccucaucc uggggaacgu ggcgcaccug aacaccaucu ucaagacguc ccucaccaag      780

gacgaggaga agcugaacuu cagcggcaag gacaucgagu ccaagcugga cgaccucgac      840

agcauccugg acgacgacca guucaccgug cuggacgccg ccaaccgcau cuacuccacc      900

aucacgcuca acgagauccu gaacggggag agcuacuucu ccauggccaa ggucaaccag      960

uacgagaacc acgcgaucga ccugugcaag cuccgggaca uguggcacac caccaagaac     1020

gaggaggccg uggagcagag ccgccaggcc uacgacgacu acaucaacaa gcccaaguac     1080

ggcaccaagg agcuguacac gagccugaag aaguuccuca agguggcccu gcccaccaac     1140

cuggccaagg aggcggagga gaagaucucc aaggggaccu accucgucaa gccccggaac     1200

agcgagaacg gcguggugcc guaccagcug aacaagaucg agauggagaa gaucaucgac     1260

aaccaguccc aguacuaccc cuuccugaag gagaacaagg agaagcuccu gagcauccug     1320

uccuuccgca uccccuacua cgucggcccc cugcagagcg ccgagaagaa cccguucgcc     1380

uggauggagc gcaaguccaa cgggcacgcc cggcccugga acuucgacga gaucguggac     1440

cgcgagaagu ccagcaacaa guucauccgg cgcaugaccg ugacggacag cuaccucguc     1500

ggcgagcccg ugcugcccaa gaacucccug aucuaccagc gguacgaggu gcucaacgag     1560

cugaacaaca uccgcaucac cgagaaccug aagaccaacc cgaucgggag ccggcucacc     1620

gucgagacga agcagcgcau cuacaacgag cuguucaaga aguacaagaa ggugaccgug     1680

aagaagcuga ccaaguggcu caucgcccag ggcuacuaca agaaccccau ccugaucggc     1740

cugucccaga aggacgaguu caacagcacc cucacgaccu accuggacau gaagaagauc     1800

uucgggucca gcuucaugga ggacaacaag aacuacgacc agaucgagga gcugaucgag     1860

uggcucacca ucuucgagga caagcagauc cugaacgaga agcugcacuc cagcaaguac     1920

uccuacaccc ccgaccagau caagaagauc agcaacaugc gguacaaggg cugggggcgc     1980

cucagcaaga agauccugau ggacaucacg accgagacca acacgcccca gcugcuccag     2040

cuguccaacu acagcauccu ggaccucaug ugggcgacca acaacaacuu caucuccauc     2100

augagcaacg acaaguacga cuucaagaac uacaucgaga accacaaccu gaacaagaac     2160

gaggaccaga acaucuccga ccuggucaac gacauccacg ugagcccggc ccucaagcgg     2220

ggcaucaccc aguccaucaa gaucgugcag gagaucguca aguucauggg gcacgccccc     2280

aagcacaucu ucaucgaggu gacccgcgag accaagaagu ccgagaucac cacgagccgg     2340

gagaagcgca ucaagcggcu gcagagcaag cugcucaaca aggccaacga cuucaagccc     2400

cagcugcgcg aguaccuggu ccccaacaag aagauccagg aggagcucaa gaagcacaag     2460

aacgaccugu ccagcgagcg gaucaugcug uacuuccucc agaacggcaa gucccuguac     2520

agcgaggagu cccugaacau caacaagcuc agcgacuacc agguggacca cauccugccg     2580

cgcaccuaca uccccgacga cucccuggag aacaaggccc ucgugcuggc gaaggagaac     2640

cagcggaagg ccgacgaccu gcuccugaac agcaacguca ucgaccgcaa ccuggagcgc     2700

uggaccuaca ugcucaacaa caacaugauc ggccugaaga aguucaagaa ccugacccgg     2760

cgcgugauca cggacaagga caagcucggg uucauccacc ggcagcuggu gcagaccagc     2820

cagaugguca agggcguggc caacauccug gacaacaugu acaagaacca ggggacgacc     2880

ugcauccagg cccgcgccaa ccucuccacc gcguuccgga aggcccugag cggccaggac     2940

gacaccuacc acuucaagca ccccgagcug gugaagaacc gcaacgucaa cgacuuccac     3000

cacgcccagg acgccuaccu cgcguccuuc cuggggaccu accggcugcg ccgguucccc     3060

accaacgaga ugcuccugau gaacggcgag uacaacaagu ucuacggcca ggugaaggag     3120

cuguacagca agaagaagaa gcucccggac ucccgcaaga acggguucau caucagcccc     3180

cuggugaacg gcacgaccca guacgaccgg aacaccgggg agaucaucug gaacgucggc     3240

uuccgcgaca agauccugaa gaucuucaac uaccaccagu gcaacgugac ccggaagacg     3300

gagaucaaga ccgggcaguu cuacgaccag accaucuacu cccccaagaa cccgaaguac     3360

aagaagcuca ucgcccagaa gaaggacaug gaccccaaca ucuacggcgg cuucagcggg     3420

gacaacaagu ccagcaucac caucgugaag aucgacaaca acaagaucaa gcccgucgcc     3480

auccccaucc gccugaucaa cgaccugaag gacaagaaga cgcuccagaa cuggcuggag     3540

gagaacguga agcacaagaa guccauccag aucaucaaga acaacgugcc gaucggccag     3600

aucaucuaca gcaagaaggu cgggcugcuc ucccugaaca gcgaccggga gguggccaac     3660

cgccagcagc ugauccuccc gcccgagcac uccgcccugc ugcggcuccu gcagaucccc     3720

gacgaggacc uggaccagau ccucgcguuc uacgacaaga acauccuggu ggagauccug     3780

caggagcuca ucaccaagau gaagaaguuc uaccccuucu acaagggcga gcgcgaguuc     3840

cugaucgcca acaucgagaa cuucaaccag gccaccacca gcgagaaggu caacucccug     3900

gaggagcuca ucacgcugcu gcacgccaac agcaccuccg cccaccucau cuucaacaac     3960

aucgagaaga aggcguucgg ccggaagacc cacgggcuga cccugaacaa cacggacuuc     4020

aucuaccaga gcgugaccgg ccucuacgag acccgcaucc acaucgagug a              4071


<210>  1889
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1889
augaccaaga aggagcagcc cuacaacauc ggccuggaca ucgggaccgg cagcgugggg       60

ugggccguca cgaacgacaa cuacgaccuc cugaacauca agaagaagaa ccuguggggc      120

gugcgccucu ucgagggggc ccagaccgcg aaggagaccc ggcugaaccg cuccacccgg      180

cgccgguacc gccggcgcaa gaaccggauc aacuggcuga acgagaucuu cagcgaggag      240

cucgccaaca cggacccguc cuuccugauc cgccugcaga acagcugggu guccaagaag      300

gaccccgacc ggaagcgcga caaguacaac cucuucaucg acaaccccua caccgacaag      360

gaguacuacc gggaguuccc caccaucuuc caccuccgca aggagcugau caucaacaag      420

aacaaggccg acauccggcu ggucuaccug gcccugcaca acauccucaa guaccgcggc      480

aacuucaccu acgagcacca gaaguucaac aucagcacgc ugaacuccaa ccugagcaag      540

gagcucaucg agcugaacca gcagcugauc aaguacgaca ucuccuuccc ggacaacugc      600

gacuggaacc acaucagcga cauccucauc ggccggggga acgccaccca gaaguccagc      660

aacauccuga acaacuucac ccuggacaag gagaccaaga agcuccugaa ggaggugauc      720

aaccugaucc ucggcaacgu ggcgcaccug aacacgaucu ucaagaccuc ccugaccaag      780

gacgaggaga agcucagcuu cuccgggaag gacaucgaga gcaagcugga cgaccuggac      840

uccauccucg acgacgacca guucaccguc cuggacacgg ccaaccgcau cuacagcacc      900

aucacccuga acgagauccu caacggcgag uccuacuuca gcauggccaa ggugaaccag      960

uacgagaacc acgccaucga ccugugcaag cugcgggaca uguggcacac cacgaagaac     1020

gagaaggcgg uggggcucag ccgccaggcc uacgacgacu acaucaacaa gcccaaguac     1080

ggcaccaagg agcuguacac cucccugaag aaguuccuca aggucgcccu gcccaccaac     1140

cuggccaagg aggccgagga gaagaucagc aagggcacgu accucgugaa gccccggaac     1200

uccgagaacg gggugguccc guaccagcug aacaagaucg agauggagaa gaucaucgac     1260

aaccagagcc aguacuaccc cuuccugaag gagaacaagg agaagcuccu guccauccug     1320

agcuuccgca uccccuacua cgugggcccc cugcagucca gcgagaagaa cccguucgcg     1380

uggauggagc gcaaguccaa cgggcacgcc cggcccugga acuucgacga gaucguggac     1440

cgcgagaagu ccagcaacaa guucauccgg cgcaugaccg ucaccgacag cuaccucgug     1500

ggcgagcccg ugcugcccaa gaacucccug aucuaccagc gguacgaggu ccucaacgag     1560

cugaacaaca uccgcaucac cgagaaccug aagacgaacc cgaccggcag ccggcucacc     1620

guggagacca agcagcacau cuacaacgag cuguucaaga acuacaagaa gaucacggug     1680

aagaagcuga ccaaguggcu caucgcccag ggguacuaca agaaccccau ccugaucggc     1740

cugucccaga aggacgaguu caacagcacc cucaccacgu accuggacau gaagaagauc     1800

uucgggucca gcuucaugga gaacaacaag aacuacaacc agaucgagga gcugaucgag     1860

uggcucacca ucuucgagga caagcagauc cugaacgaga agcugcacuc cagcaacuac     1920

agcuacaccu ccgaccagau caagaagauc agcaacaugc gcuacaaggg cugggggcgg     1980

cucuccaaga agauccugac cugcaucacc acggagacca acacccccaa gagccugcag     2040

cucuccaacu acagcguccu ggaccugaug uggaccacga acaacaacuu caucuccauc     2100

aucagcaacg acaaguacga cuucaagaac uacaucgaga accacaaccu caacaagaac     2160

gaggaccaga acaucuccaa ccuggugaac gacauccacg ugagccccgc ccugaagcgc     2220

ggcaucaccc aguccaucaa gaucguccag gagaucguga aguucauggg ccacgccccg     2280

aaguacaucu ucaucgaggu cacccgggag accaagaagu ccgagaucac gaccagccgc     2340

gggaagcgga uccagcgccu ccagagcaag cugcugaaca aggcgaacgg cuucaagccc     2400

cagcuccgga aguaccuggu gcccaacgag aagauccagg aggagcugaa gaagcacaag     2460

aacgaccucu ccagcgagcg caucaugcug uacuuccugc agaacgggaa gucccucuac     2520

agcgaggaga gccugaacau caacaagcug uccgacuacc agguggacca cauccucccc     2580

cggaccuaca ucccggacga cagccuggag aacaaggccc ugguccucgc caaggagaac     2640

cagcgcaagg ccgacgaccu gcugcucaac uccaacguga ucgacaagaa ccuggagcgg     2700

uggaccuaca ugcugaacaa caacaugaug ggccucaaga aguucaagaa ccugacgcgc     2760

cgcgugauca ccgacaagga caagcugggg uucauccacc ggcagcucgu ccagaccagc     2820

cagaugguga agggcguggc caacauccug aacuccaugu acaagaacca gggcaccacg     2880

ugcauccagg cgcgcgccaa ccugagcacc gccuuccgga aggcccucuc cgaccaggac     2940

gaccgcuacc acuucaagca ccccgagcug gucaagaacc ggaacaucaa cgacuuccac     3000

cacgcccagg acgcguaccu ggccagcuuc cucgggaccu accgccugcg gcgcuucccc     3060

accgacgaga ugcugcucau gaacggcgag uacaacaagu ucuacggcca ggugaaggag     3120

cuguacucca agaagaagaa gcugcccgac agccggaaga acggguucau caucuccccg     3180

cucgugaacg ggacgaccca guacgaccgc aacaccggcg agaucaucug gaacgucggc     3240

uuccgggaca agauccugaa gaucuucaac uaccaccagu gcaacgugac ccgcaagacg     3300

gagaucaaga ccgggcaguu cuacgaccag accaucuaca gccccaagaa ccccaaguac     3360

aagaagcuga ucgcccagaa gaaggacaug gacccgaaca ucuacggcgg guucuccggc     3420

gacaacaaga gcuccaucac caucgugaag aucgacaaca acaagaucaa gcccgucgcc     3480

auccccaucc ggcucaucaa cgaccugaag gacaagaaga cgcugcagaa cuggcucgag     3540

gagaacguga agcacaagaa guccauccag aucaucaaga acaacgugcc caucggccag     3600

aucaucuaca gcaagaaggu cgggcugcug agccucaacu ccgaccgcga gaucgcgaac     3660

cggcagcagc ugauccugcc gcccgagcac agcgcccucc ugcgcauccu gcagaucccc     3720

gacgaggacc ccgaccagau ccucgccuuc uacgacaaga acauccuggu ggagauccug     3780

caggagcuca ucaccaagau gaagaaguuc uacccguucu acaagaacga gcaggaguuc     3840

cuggccagca acaucgagaa cuucaaccag gccaccaccu ccgagaagau caacagccug     3900

gaggagcuca ucacgcugcu gcacgcgaac uccaccagcg cccaccucau cuucaacaac     3960

aucgagaaga aggccuucgg ccggaagacc cacgggcuga cccugaacga cacggacuuc     4020

aucuaccagu ccgugaccgg ccucuacgag acccgcaucc acaucgagug a              4071


<210>  1890
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1890
augagcaaga aggacuccga guacuacauc ggccuggaca ucgggaccaa cagcgugggc       60

ugggccguca ccaacgggaa guacgaccuc cugaacauca agaagaagaa ccuguggggc      120

gugcgccucu ucgaggccgc ggaguccgcc gccgggcggc gcaucaaccg gagcacgcgc      180

cggcgcuacc ggcgccggcg caaccggauc aacuggcuga acgagaucuu cgccgacgag      240

cuguccaagg uggaccccag cuuccucacc cgccugaacu ccagcuggau cuccaagaag      300

gacagcuccc ggacccgcga caaguacaac cuguucaugg acgccgacua caccgacaag      360

aacuaccacc acaaguaccc gacgaucuac caccuccggc gcgaccugau guucgacgac      420

aagcaguucg acauccggcu ggucuaccuc gcgcugcaca acaucaucaa guaccgcggc      480

aacuucaccu acgaccaccg gaaguucaac gugagcgaga uguccagcaa ccugaccaag      540

gcccucgaga acuucaacca ggagcugaug gaguucgagg acgccuuccc cgacaacacc      600

gacuacuccg ccaucagcga ggugcugcuc aaguccaaca acgucagcac gaagaucgac      660

gagaucaucu ccaaccugga gcuggacaag gacaccaaga aguaccucaa ggaggugcug      720

aagcugaucc ucggcaacag cgcgaaccug aacaccaucu uccacaccag ccuggacaag      780

gacgacgaga agcucucccu gagcgccaag gacaucgagc ugaagcucaa caaccuggac      840

ucccugcuca gcgacgagca gauccacuuc aucuccagcg ccaacgagcu guacuccagc      900

aucacgcuga acgacauccu cgacgggaag accuaccugu cccuggccaa gaucaagcag      960

uacgacaccc acugcagcga ccuccaggag cugaaggaga ugugguacaa gaccacgaac     1020

cccgaccuga ucaagaaguc ccgcaaggcc uacaaggagu acgugcacgg cagcuacaac     1080

accgaggacu ucuacaaggc gaucuccccc uuccucaaga ucgccauccc ggaggaccug     1140

gccaaggacg cccugaagaa gaucgacgcc aacaccuacc uccccaagca gcggaccagc     1200

aacaacgggg ucauccccuu ccagcugaac cugaucgagc ucgagaagau caucgacaac     1260

caguccaagu acuaccccuu ccugaaggag aaccgcgaga agaucaucag cauccugucc     1320

uuccgcaucc cguacuacgu gggccccauc caggcgccca cggacaagaa cuucagcugg     1380

auggugaaga agauccaggc caaggcccgg cccuggaacu ucgacgaggu caucgaccgc     1440

gagaagucca gcaacaacuu caucaagcgg augaccacca ccgacacgua ccuggugggg     1500

gagccggugc uccccaagaa cagccugauc uaccagaagu ucgagguccu gaacgagcuc     1560

aacaacaucc gcaucuccaa gaaccugagc caggaccccc ugggcuccaa gcucagcgug     1620

gaggugaagc agcagaucuu cgaggagcug uucaagcggu acaagaccgu caaguccgac     1680

cagcugaaga aguggcucau ccgcaacagc uacuucaccg accccaccau caucggccug     1740

uccaagccga gcgaguucaa cuccagccug uccacguuca ucgacaugaa ggccaucuuc     1800

gggauggacu ucgugaacaa cccccggaac agcucccagc ucgaggagcu ggccgagugg     1860

cugaccgugu ucgaggacaa gcagauccuc agcgagaaga uccgccacuc caacuacccc     1920

uacagcgacc agcagauccg gaagaucucc aacaugcgcu accggggcug ggggcgcauc     1980

agcaagaagc ugcugugcga caucaagguc gagagcacca ucuacaaguc ccaccagcuc     2040

caccccuaca gcauccugga ccugaugugg uccaccaaca acaacuucua cagcauccuc     2100

cacaaggaca aguacaacuu cgaguccauc cuggaggacu acaaccuggg cacggacgac     2160

aagaccgaca ucaacgagcu cguggacaac auccacacca gcccggcgcu gaagcggggc     2220

aucauccagu ccaucaacau cgugaaggag cuggucaagu ucauggggca cgcgccccag     2280

cacaucuucc ucgaguucac ccgcgaggac aagacgagca cccuguccga gagccggaag     2340

aagcgccuga agaagcucua caacaaccug uccgaggaca ccaagaaguu caagcuggac     2400

cuccgggacu accuggugcc cgacaagagc auccagaagg agcuggaguc ccacgacaag     2460

gaccucagca aggagcgccu gaugcuguac uuccuccaga acggcaaguc ccuguacagc     2520

gacgaggccc uggacaucaa ccggcucucc gaguaccagg ucgaccacau ccugccccgc     2580

accuacauca aggacgacag ccuggagaac aaggcccucg ugcuggccaa ggagaaccag     2640

cgcaaggccg acgaccugcu ccugcagccg uccaucauca acaagaacau ggagcggugg     2700

aucuacauga aggaccacgg gaugaucagc cccaagaagu ucaagaaccu gacccgcacg     2760

accaucaccg aggacgacaa ggugcacuuc aucaaccggc agcucgucca gaccagccag     2820

aucaucaaga acguggccaa cauccuggac aacauguaca aggagcaggg cacgaccugc     2880

guggagacca aggccaaccu guccagcgcc uuccgcaccg cguucuccga gcaggacgac     2940

aaguaccacu ucaagcaccc cgaguucauc aagaaccggg acgucaacga cuuccaccac     3000

gcccaggacg ccuaccucgc cugccugcug gggcucuacc gcauccagcg guuccccagc     3060

gacgagaugc ugcugaucaa gaaggaguac cgcaaguucu ucgcccaggc gcagaacucc     3120

gugaagaacc acggcaagau cccggacagc cagaagaacg gcuucauccu cgcgccccug     3180

gugaacgggg agacgcugau caacaacaag accauggaga ccaucuggaa cauggacuac     3240

aagcggaagg ucaucaagau cuucaacuac aagcaguaca acaucaccaa gcgcacggag     3300

aucaagaccg gcgaguucua caagcagacc cucuacagcc cccacgacga gaacgugaag     3360

aacccgaucc cccagaagaa gaaccuggac cccaagaucu acgggggcua cuccggggug     3420

caguccagcu accuggccau caucaagguc gaccagaaga agcccaccau gguguccgug     3480

ccgauccggu ucagcaagau gaucgacgac ggcaagcucu cccugcagag cuggcuggag     3540

aacaacauca agcacaagaa gacgaucgag guccucaaga ccaagauccc caucggccag     3600

cugaccuaca accccaagaa cggguaccug uccgugcaga gcgagaagga gaucgccaac     3660

gcccagcagc uguuccuccc cuucgagcac guggcccugc ucacccugcu gcacgccccg     3720

acguccgagu acgaccagau ccucagcuuc uacgacaccg acauccugcu ggagaucuuc     3780

gacgagauca ccaacaagau caagaccuuc uuccccuucu acaucgacga gcaggaguac     3840

cucgucucca uccgcgagaa cuuccagagc gccacgguga ucgagaaggu gaacguccug     3900

cagcagcugc ucaucaugcu gcacgccaac agcucccgga ucagcaucaa gcugggcgag     3960

guguccaaga aggaccucgg gcgcaagaag aagcggaucc cccuggagaa caccgaccug     4020

guguaccaga gcgucaccgg ccucuaccag accaaguucc acaucgacug a              4071


<210>  1891
<211>  4074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1891
augaacgacc gcaacaagaa guacaacauc ggccuggaca ucgggaccgg cagcgugggg       60

ugggccguca ccgacaacaa cuacaaccuc cugcacgcca agaagaagaa ccuguggggc      120

auccggcugu ucgacggggc ggagacggcc gccucccgcc ggaccuuccg cagcacccgg      180

cgccgguacc gccggcgccg gaaccgcauc aacuggcuga acgagaucuu cgccgacgag      240

cuggccaaga aggaccaguc cuuccucgug cggaugaaca gcuccugggu gagcaagaag      300

gacaagaucc gccagcggga ccaguacaac cuguucaugg acuccgacua caccgacguc      360

gaguaccacc gcgaguuccc cacgaucuuc caccuccgga agcgccucau cgaggacaag      420

agccaggcgg acauccggcu gguguaccug gcccuccaca acaucaucaa guaccgcggc      480

aacuucaccu acgagcacca gaacuucgac gugucccaca uggacagcgg ccuggcccug      540

cuccugaacc agcugaacca gcagcuccag gccuucgagg ucgcguuccc ggacgagacc      600

aacuucgagu ccaugagcac cauccugcug gagaagaaga cgcccaccca gaagaucgac      660

gagaucgugg accagaucca gcccgagaag gaccuccugc agaucaccaa gaacaucaug      720

aagaugcugc ucgggaacaa ggccgaccug gugaagaucu ucucccugga cagcgacgac      780

aagaucaccg ucgaguucuc cagcaacucc aucgagcagg accucagcaa ccuggaggag      840

acgcuguccg acgagcaguu caacaucauc acccucgcca acagcaucua cagcuccauc      900

gugcugaacg acauccugag cggcgagacc uaccucuccu ucgccaaggc ccggcaguac      960

agcgaccaca agaaggaccu ggcgaagcug aagaccaugu ggcgcgaguc ccccgaccgc     1020

aaggccgugg ggaaggccaa gaaggccuac gacgacuacc uccacagcgg caaguacggg     1080

aucgaggacu ucuacaagga cauguccaag uuccuggaga cggccacccc ggucgagcug     1140

gcgaaggagg ccaacgagaa gauccucaac aagagcuacc ugcugaagca gcggaacaac     1200

gagaacggcg ugauccccuu ccagcucaac aagaacgaga ugaucgagau caucaacaac     1260

caggccaagu acuaccccuu ccuggccgag aacaaggaca agcugcucuc cauccugagc     1320

uuccgcaucc ccuacuacgu gggcccgcug caguccaaga aggacaaccc cuucgccugg     1380

auggagaaga agucccaggg gaagauccgg cccuggaacu ucgacgagau cgucgaccgc     1440

gaggcgagca gcaacaacuu cauccggcgc augaccucca ccgacacgua ccucaucggc     1500

gagcccgugc ugccgcucaa gagccccauc uaccagaagu acgaggugcu gaacgagcug     1560

uccaacgucc ggaucaacgc cgacgaggug gacaaccccc ugggggacaa gcugagcgug     1620

gaccucaagc aggccaucua caacgagcug uuccugaagc accgcuccgu cagcaaggcc     1680

cuccugacca aguggcugau caaccagagc uacuuccaga acccccagau cuccggccuc     1740

gccgacagca agaaguucaa cuccggccug accaccuacc acgacuucaa gaagaucuuc     1800

ggggaggacu ucgugaacga cccgacgaac cagagccagc uggagcagcu cgcggagugg     1860

cugaccaucu ucgaggacaa gaagauccug aagaucaagc ucgacgacuc ccaguucaac     1920

ugcacccccg agcagaucaa gaagaucgcc ggcaugcggu accaggggug gggccgccug     1980

agcaagaagc ugcucugcga caucaagacc acgaccguga ccuccaagaa ccaccagcag     2040

gccagcuacu ccauccugga ccugaugugg aacaccaacc ggaacuucau cagcguccuc     2100

aagaacgaca cguacaaguu cgaggacaag auccagaccu ucaaccugga caagaacgag     2160

gccgcgacgc cccaggccau gaucgacgag cugcagaccu cccccgcccu caagcgcggg     2220

auccggcaga gcgugaccau cauccaggag cuggucaagu ucaugggcca cgccccggag     2280

cacaucuucc uggaguucac ccgcgaggac gacgugucca gccucaccaa gucccgggac     2340

agccgccuga acaagcugua cgcccggauc gcgaagaaca ccaagaagac cgacgacgag     2400

cucaagaagu cccugcugcc cagcagcgac aucaaggcca cgcucaaggc caacaagaac     2460

aaccugucca acgaccgccu gaugcucuac uaccugcaga ugggcaagag ccuguacucc     2520

aaccagcccc ucgacaucaa ccggcugagc gaguaccaga ucgaccacau ccugccccag     2580

uccuacauca aggacaacag ccucgagaac cgcgcccugg ugcuggccuc cgagaaccag     2640

cacaagagcu ccgaccuccu gcugaagccg gagaucauca accagaacau cgcgcgcugg     2700

cgguacauga aggacgccgg gcucaugggc cccaagaagu ucaagaaccu gacccgcauc     2760

cagaucaccg acagcgacca gaacaacuuc aucaaccggc agcuggucca gaccucccag     2820

aucaucaagc acgugagcaa cauccucgac uccauguacg gggaccaggg cacgcagugc     2880

guggagaccc gcgcccaccu gagcaccgag uuccggaagg ccuucuccaa cgaggacgac     2940

aacuaccacu uccagcaccc cgaguucguc aagaaccgca acaucaacga cuaccaccac     3000

gcccaggacg cguaccuggc cugccuccug gggcuguacc agaugaagaa guaccccacc     3060

gacaacauga uccuccugaa gaaggaguac aagcgguucu ucagcaacac gaagaagauc     3120

uacgacaaga agcaccacuu cccggacuac gugaagaacg gcuggaucau cggcuccaug     3180

uucaacgggg agaaguacgu ggacgaggag accggccaga ucaucuggga ccagcagacc     3240

aaggacaugg ucagcugcau cuuccaguac aagcaguaca acgugaccaa gcgcacggag     3300

gccgccaacg gggaguucua caaccagacc guguacaaga aggaccccaa cgagaagcgg     3360

aagcugaucu cccucaagca gggccuggac accaagcugu acgggggcua cagcggcgac     3420

aaccccgcgu acaucguccu cgugaagauc gacgacaaga agaacaagcu ggugaagaug     3480

ccgauccgcc uggccgagca ggucgacaac aagaccaucg accuccagac guggcuggag     3540

gagaacgugc cccacaagaa ggggauccag gugcugaaga ccaagauccc ccucggccag     3600

gucaucuaca gcaagaagaa cggguaccug gcccugaagu ccgacaccga gaucgugaac     3660

gcccagcagc ucgugcugcc cuacggcuac gucgcccugc ucacccugcu gcagaagaug     3720

ccggagaaca aguacgacga ggugcucgcg ugcuacgacg acggcauccu ggacaacauc     3780

uucguggcca ucaucgacaa gaugaagcac uucuaccccu acuacagcgg ggagcugaag     3840

acgcucuccg acaacaucga caacuucaag gagagccccg acgucaacaa gaucaagacc     3900

cugcagcagc ugcuccugcu gcuccacgcc uccagcacca ccgccuccau cgaguucaac     3960

aacgugaaga agaacagccu gggccggaag cccaacucca ucaacaucca gaacacggac     4020

cugcucagcu ucuccgccac cgggcuguac gagagccgca uccacaucga cuga           4074


<210>  1892
<211>  4074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1892
auggccgcca agaacuacaa caucgggcug gacaucggga ccagcuccau cggcugggcg       60

aucguggacg acaacuacca gcucaugcac gccaagggga aguacggcua cgggacccgc      120

aucuacagcg agggccagac ggccgccgac cggcgcggcu uccggaccac ccgccggcgc      180

cugaaccggc gcaaguggcg gcugcgccuc cugcaggacu ucuucgagcc cuacauccug      240

ccgcaggacg aggccuucuu cauccggcag aagcagucca accucguccc ccaggacacc      300

aagaagcagu accccgagag cgugcuguuc gacgacacgu ccgacaagga cuucuacacc      360

gaguacccca ccaucuacca ccuccgccag gcgcucauga ccgagaaccg gcaguucgac      420

auccgcgagg uguaccuggc cauccaccac aucgucaagu accgggggca cuuccugaac      480

aacgccccgg ugaagacguu caagagcggc aaccucgacc uggccaucaa guucaacgag      540

cugaacgagg aguucaaguc cguguacccc gaccucgagc ugcaguucaa ccuggaccag      600

aucgacgaga ucaagaagau ccuccuggac agcaccaagu cccgcuucga ccggcagggg      660

gaggcgguca agcugaucua caccgugagc gaggugaaga ccaucgagaa gcuccagaac      720

aagaucguca agcaguuccu guccggcauc cuggggcuca acacgcgcuu cgacgugcug      780

accaacaccg agagcgacga cuccaugugg aaguucagcc uggacuccga gagcuccgac      840

gacgcccucg acgagaucau cgcccagcug gacgaccccg ccauccacgu ggucgagcug      900

gugaacgaga uccacagcuc caucacccuc gccggcaucg ugcccgaggg ccaggggcug      960

agcgagagca uggucgagaa guacgaggcg cacaaggccc accuccagcu ccugaaggcc     1020

guggagaagc ugguggacaa gaaggccgcc gacaagcucg aggucgcgua cgaccaguac     1080

aucaacggca aggacuccaa gcaguucauc aaggaggacu ucuacaaggc cgugcagagc     1140

gagaucaagg ggguggccgg cgacgagguc gccgagauca accagcugau cgagcuggac     1200

caguucaugc cgaagcagcg gacgaaggag aacggcgcca ucccccacca gcuccaccag     1260

cacgagcugg acgugaucau cgagaaccag uccaaguacu acgacuggcu gggggagaag     1320

gugggcaacg aguacaagcu cgacaagcug gucagcuucc gcgugcccua cuacgucggg     1380

ccccuggugg acaccgcguc caaggagaag aaggacgaga cccgguucgc cuggauggug     1440

cgcaagaacc cggagagcac cgaggccauc acgcccugga acuucgagga caucgucgac     1500

aagaacgccu ccgccgagaa cuucaucaag cgcaugacca ccaaggacaa guaccuccug     1560

aacgaggacg ugcugcccaa gugcagccuc cugacccagg acuucgaggu guacaacgag     1620

cugaacaaca uccgggucaa cggcgagaag uucacggcgg agcagaagca gcugauccac     1680

gacgagcugu ucaccaagca cgccuccgug agcaucaagc agcugaagga cuuccuggug     1740

uccgagcuca uguacguccg cgacaaccug aagguggagg ggcuggccga cgagaagaag     1800

cucaugagcu cccugagcac ccaguucgac uaccagaaga ucauccccga ccagaucaac     1860

gacgugaagu accgggagga caucgagcag aucaucgagu gguccaccau cuucgaggac     1920

gccgcgaucu ucaagacgaa gcugaaggag aucgacuggc ucaccgacga gcagcgcaac     1980

aagcuggcca acaagcggua ccgcggcugg ggcagccugu ccaagcggcu ccuggccugc     2040

cuggucgacg agaacgggca gcgcaucauc gacauccucc gggacagcuc cgacaacuuc     2100

augaugaucg ugaaccgccc ggaguucaag caggccaucu ucgacgagaa cgccaaccug     2160

accgagacca acagcaagaa ggacauguuc gagaugaucg acaacuucua cacgagcccc     2220

cagaacaaga aggcgauccg ccaggugcug cucgucgugc aggacaucca gaacgccaug     2280

ggcggggugg cgccccagaa gaucuucauc gaguucgccc gggaggacga ggucaacggc     2340

cggcgcacca acucccggua cagccagauc cgcgagaugu acuccaaggu gaccgacgac     2400

gugaucaaca agaccguccg cggggagcug gaggccaaca agggcagccu gaacaaggag     2460

aagcuguucc uguacuuccu gcagggcggg aucgaccucu acuccggcaa gacgaucaac     2520

aucgacaaca ucagcuccua cgacguggac cacgugcugc cccagagcuu caucaaggac     2580

gacuccauca acaacaaggu ccuggugaac gccaagggga accgggugaa gaacgacaac     2640

gucccgaugg agcuguucgg cgccaagaug uaccccaccu ggaagcggau gguggacacc     2700

gggaucauga gcaaggccaa guucaagaac cucacccuga accccgacaa cguggacaag     2760

uucaaggccg agggcuucau caaccgccag cucgucgaga cccgccagau caucaagcug     2820

guggcccaga uccugaacga caagcucggc gacgacacga agaucguguc cgucaaggcg     2880

aaccugaccc acgacaugcg gguguucuuc gacuucccca agaaccgcaa cgugaacaac     2940

uaccaccacg ccuucgacgc cuaccugagc gccuucgucg ggaccuaccu cuacaagcgg     3000

uacccgaagc ugcagcccua cuucguguac ggcgaguuca agaagaugga caagcugaag     3060

aacaugaccu ccuucgacuu ccuccgcgac cugaagcaca cggacaaggu ggucgacaag     3120

gacaccgggg agaucaucgc ggacaagcug gccgugauca ccaacaugaa caagaucuac     3180

gacuucaaga aggugcucgu cacccacgag gugcacgaga acagcggcgc cauguacgac     3240

cagacgcugu accccgccaa gcuggacaag ucccucggcc ggaagggggc caagcagcug     3300

auccccaaga aggacgacau gccgaccgcg cuguacggcg gguacaccgg cagcaaggac     3360

gccuucaugu ccaucgugcg ccucaagaag aaggacgagg ucuacuacaa ggugaucggg     3420

auccccgugc ggaucgccga cagcguccag cacggcaagg agguggacgu ggaggcccug     3480

acccaguuca ucgagacgaa guucaccaag gagaaccuga acaagaagac cggcgagauc     3540

acccgcaagg ucgagugguu cgagcucgug cugcccaagg uccuguucgg gcaggccgug     3600

uucgacggcg ggcagccguu caugcucggc agcuccacgu acccccacaa ccugcgggag     3660

cuggugcuca ccaaggacag cgucaagaag aucgcgggga aggaguccaa caagagcuac     3720

cuggacgccu acgacgagau cauggaggcc augaacaagu acuucccccu guacgacacc     3780

aacaaguucc gcgagaaggu gaucgagggc cgguccaagu ucaucgaccu ccccgacaag     3840

gacgaguacg agaacaacaa gcuggugcag gucggcaaga gcaccaucau cgaccgcaug     3900

uucaucgggc ugcacgccaa cgccgcgcgg acggaccuca agaacaucgg caucaccacc     3960

ccguucgggu ucaugcagca ggccuccggc aucaagcuga ccaaggacac gguggugcug     4020

uuccagagcu ccaccggccu cuucgagcgc aaggucgccc ugaaggaccu guga           4074


<210>  1893
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1893
augaaggagc ccuacggcgu ggggcuggac aucggcacca acagcgucgg guggaccgug       60

guggacgccu ccggccacgu ccgcaagauc aaggggcaga cgggcaucgg cgugcggcug      120

uucaaggagg gggccgcggc cgccgaccgc cggggcuucc gcaccacccg gcgccggcug      180

aagcgcguga aguggcggcu gcgccuccug cgggaguucu ucgaccagcc gaucagcaag      240

gucgacauca acuucuucgc ccgccggaag uacuccgacg ugagcccccg cgaccccaac      300

uacaacgggc uggagaagac ccucuucaac gaccgguccg accaggacuu cuaccacgac      360

uaccccacga ucuaccaccu ccgcgaggcc cugaugaccc agcaccggaa guucgacgug      420

cgcgagaucu accucgcgau ccaccacauc gucaaguacc ggggcaacuu ccugcgcaac      480

gacgccgcca ccgccuaccg gagcgggacc cuggaccucc agcagcacuu cgagacgcug      540

aaccaccugu ucucccaggc ggaccucgag cugaaccuga accucaccac cgacguggcc      600

cugcuggaca gcaucaagca gacccucgug cgcacggaca ucucccgcag cgaccggcag      660

aagcugauca ugccgcugcu cgccguccug accggcgcca ccaccgccga gaagaagcgc      720

cagaaggcgg uggugacgga guucgccaag gcccuggucg gcaacaagac caagaucgac      780

gugcucaccc ugaccgacau cgacgccacg gaggccaagg acugggcguu cucccuggag      840

gagaaccagg acaagcuccc cgggaucgag gaccgguuca gcgagguggg ccagcagauc      900

aucgacgagg ucauccgccu guacgccucc gugaaccugg cccagcucau ccccgagggg      960

aagcgguuca gccaguccau gguggagaag uacaagcgcc acggcgagga ccugaagcug     1020

cucaaggccu acauccggag ccaguccgac gccaagcgcg gccgggcgau ccgcgccacc     1080

uacgaccagu acaucgacgg ggucaagagc aagcagguca cccaggaggc cuuccagaag     1140

gcccugggca aguucgugga cgccgacgug gaccugaacu ccugggcggc ccagaucaag     1200

caggccaucg acagcgagca guucaugccc aagcuccgga ccaagcagaa cggggccauc     1260

ccguaccagg ugcagcagaa cgagcuggac cagaucauca gcaaccagaa ggaguacuac     1320

cccuggcugg gcgaggagaa ccccgucauc aagcgccggg ggaaguuccc cuacaagcuc     1380

gacgagcugu ucggcuuccg cgugccguac uacgugggcc cccugaucac gaaggagaug     1440

caggcggcca ccgccaacgc cggguucgcc uggauggucc ggaaggcgga cggccccauc     1500

acccccugga acuucgacga caagguggac cgcguggccu ccgccaccgc cuucauccag     1560

cggaugcaga cgaccgacac cuaccucauc ggggaggagg uccugccggc ccgcagccug     1620

aucuaccagc gguucauggu gcucaacgag cugaacaaca uccgcgugga cgacggccag     1680

cugacgcccc gccagaagca gcggcuguac aaccaggucu ucaagaagca caagcgcgug     1740

accgcgaaga acauccagga caaccucgug gccgcccggg aguuccccac cgcgccccgc     1800

aucaccgggc uggccgaccc gaaggucuuc aacuccagcc uguccaccua ccacgaccuc     1860

aagcaggucc ugcccgaggc caugagcgac gugcggaagc aggccgacau cgagaagauc     1920

auccuguggu ccacgaucuu cgaggacagc accaucuuca agcagaagcu caccgagauc     1980

uccuggcuga ccgccgccca gcugaagcag cucggccagc ugcgcuaccg gggcuggggg     2040

cagcugcucc gcaagcugcu gaccggccuc aaggacgaca acaaccggag caucauggac     2100

gggcuguggg agacgaacgc gaacuucaug cagcugcgcu cccagcccgc caucgagaac     2160

cagaucaaga gcgccaacca ggccaaccuc accaccgccg accugcagga caccaucaac     2220

gagcuguaca cgagccccca gaacaagaag gcgauccggg aggugauggu ggugcucgcc     2280

gacaucaagg acgccaugca cggccagacc ccgaacugga ucuucgucga ggccgcccgc     2340

gggggcggcg uggcggggcg gcgcacccag ucccggucca agcagaucgu ggccgccuac     2400

cagggcaccg cccaggccau cguccgcgac caggugcagc gggagcugaa ggagaagauc     2460

aagaacaagg cgaacuucaa cacgcgccug gugcucuacu uccugcagaa cgggcgggac     2520

cuguacaccg gcgacgccau caacaucgac cgccucagcg aguacgacau cgaccacauc     2580

cugccccagu cccuggucaa ggacgacagc cucgacaacc gggugcugac caacgcccgc     2640

aucaaccgcg agaagaacga caccuucgcc uccgagaagu ucggcaagaa gaugggggcg     2700

aaguggaagg agaugcaccg gaugggccug augacgaagc gcaagcucga ccaccuccug     2760

augcggcccg acgagaucag caaguacgcc accggguuca ucaaccgcca gcugguggag     2820

acccggcagg ucaucaagcu gguggaggag cugaucaacg ccgaguaccc cagcaccgcc     2880

aucguguccg ucaaggccaa ccucacgcac caguuccgcc agaccuucga cuucccgaag     2940

auccgggagg ugaacgacua ccaccacgcg uucgacgccg cccugaccgc cuucaucggc     3000

accuaccugc ucaagcgcua ccccaagcug gagcgguucu ucguguacgg gaaguucgcc     3060

aagcagcccg ucaaccugac gcgcuucaac gugauccgga agcucgcgaa gaccgacacc     3120

ccgaucaccg acaucgacac gggcgaggug cugugggaca agcugaccga caucaaguac     3180

uucgagaccc ucuacgacua caagcgccug cuggucaccc gggaggugag cgagaacuac     3240

ggcgccaugu ucgaccagac gcuccugaag gccuccgaca acgggaagcg ccugauccgg     3300

aagaagcaga gcuucgacac cgccaucuac ggcggguaca ccggcaagac ccucgccuac     3360

auggcgaucg ugaaggucuc cacgaagaag gacgugcagu accagguggu cggggugccc     3420

accaccaugg ugacccagau cgagcagcug gagcagcagg gcaugacgaa caagcaggcc     3480

gucaccaagg ugaucgagcc ccugaucgcc gccaacaaca agaagaugcu caccuacgag     3540

gugcugcugc ccaagguccg cuucgagcag gucgugcggg accgcaucaa gggccagacc     3600

caccgguucg cccucgggac cgacacguac uaccacaaca uccaggagcu guaccugccg     3660

cucacccugc agcgcgcguu ccugggcaag cagaacgaga gcgaggugca gcucaaccag     3720

aaccugcuga ccguguucga cgccacccuc gcccagguca accgcuacuu cccccuguac     3780

gagaugaaca aguucaagaa gauccugaac uccgcccggg accagaucga gcuccuggag     3840

gcgaagaacc aguucgugaa gaacaagcug gugcucggga agcagggcau ccugacgaac     3900

auguucaucg ggcugcacgc caacgccagc uacggcgacc ucaagguccu gggcgugaag     3960

aucgacuucg ggaagcucca ggugcccggc gggaucaagc ugaccggcga cgccgagauc     4020

aucuaccagu cccccaccgg gcuguucgag cgcaagaucg cccugaagga ccucuga        4077


<210>  1894
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1894
augaacgagc ccuacggcgu ggggcuggac aucggcacca acagcgucgg guggaccgug       60

guggacauga acggccgcgu ccggaaggug aaggggaaga cggcccucgg cgcccgccug      120

uucaaggagg gcgcgaccgc cgaggaccgg cgcggguucc ggaccacccg ccggcgccug      180

aagcggguga aguggcgccu ccggcugcug cgcgaguucu ucgaccagcc gaucuccaag      240

aucgacccca acuucuucgc ccggcgcaag uacagcgaca ucagcccccg ggaccccaac      300

uacaacggcc ucgccaagac gcuguucaac gaccgcaccg accaggaguu cuaccacgac      360

uacccgacca ucuaccaccu ccgggacaag cugaugaccu ccgaccgcaa guucgacauc      420

cgggagaucu accuggccau ccaccacauc gucaaguacc gcgggaacuu ccugcggacg      480

ggccccgcgu cccaguacgg gagcgccucc cuccaccugg ccaccagcuu ccagaagcug      540

aacgaccucu ucgcccaguc cgaggagagc cugaacuuca agcuggugac cgacgaggcg      600

cagcuccagc agauccagca gauccuggug cgcaccgacc ugucccgcag cgagcagcag      660

cggcagaucu ggccccucau ggucgugcug augggcgcca cggccgccga gaagaagcgc      720

cagaaggacu ucgccaagga gcuggcgaac gcccucgugg gccugaaggc caagcugaac      780

gucgugaccc ucaccaacgu ggacaccacg gucgugaagg acuggaccuu cacccuggag      840

gagucccagg acaagcugcc cgagaucgag gagcagcuca gcgagguggg gcagcagauc      900

auggacgagg ucauccagcu guacgccucc gugaaccugg cccagcucau cccggcgggc      960

aagcgguuca gccagcacau gguggagaag uacaaccacc acgagaagaa ccuggagcug     1020

cucaaggccu acauccacuc ccagagcgac uccaagcgcg ggcgggagau ccgcgccacc     1080

uacgaccggu acaucgacgg cgucaagagc aagcccguga cgcaggagau guucuacaag     1140

gaccugauga aguacgugga ggccgacgcc accagcaacc accuggcggc cgagaucaag     1200

gacgagaucg acuccgagca guucaugccc aagcuccgca ccaagcagaa cggcagcauc     1260

cccuaccagg uccagcagua cgagcuggac cagaucaucu cccaccagaa gaaguacuac     1320

ccguggcugg gggaggagaa ccccguggcc gagcggcgcg gcaaguuccc cuacaagcuc     1380

gacgagcugg ucggguuccg ggugcccuac uacgugggcc cgcugaucac caaggaggac     1440

cagcaggcca cgagcggggc cggcuucgcg uggauggucc gcaaggccga cggccccauc     1500

acccccugga acuucgacca gaagguggac cggaucgccu ccgccaccgc guucauccag     1560

cgcaugcaga ccacggacac cuaccucauc ggggaggacg ugcugcccgc ccggagccug     1620

aucuaccagc gcuucauggu ccucaacgag cugaacaaca ugcgggugga ggaccagaag     1680

cuggccccgc gccagaagca gcggcuguac aaccaggugu ucaagcagca ccagcacguc     1740

uccgugaaga acauccagca gaaccucaug gacgccggcg aguaccgcga gacccccaag     1800

aucaccgggc uggccgaccc caagggcuuc aacagcuccc ugagcacgua ccacgacuuc     1860

aagaagaucc uccccgaggc gaucgccgac gagcacaagc gcgccgacau cgagaagauc     1920

auccuguggu ccaccaccuu cgaggacagc gccaucuuca agcagaagcu ggaggaggug     1980

gccuggcuca ccgacgcgca gcggaagcag cuguccgggc ugcgcuaccg gggcuggggc     2040

cagcucagcc acaagcugcu gacggccuuc aaggacgaca acgggcgcuc cgucauggac     2100

ggccucuggg agaccagcga caacuucaug cagcugcgga agcagccggc caucgagacc     2160

cagaucaagg aggccaacca ggccaaccug accgacgcgg acauccagga caccaucaac     2220

gagcucuaca cgagccccca gaacaagaag gccauccgcg aggugaugcu ggugcuggac     2280

gacaucaaga acgccaugca cgggcagacc cccuccugga ucuucaucga ggccgcgcgg     2340

ggcgggggcg ucgccggccg ccggacccag ucccgcagca agcagaucgu ggaggccuac     2400

aagagcaccg ccaaggagau cguguccgag aagguccagc acgagcucaa cgagaagauc     2460

aaggccaagg cggacuucaa cacgcggcug gugcuguacu uccuccagaa cgggcgcgac     2520

cuguacacca acgaggccau caacaucgac cggcugagcg aguacgacau cgaccacauc     2580

cucccccagu cccuggugaa ggacgacagc cuggacaacc gcguccucac cuccgcccgg     2640

aucaaccgcg agaagaacga caccuucgcc agcgagaagu ucggccggaa gaugggggcc     2700

caguggcgcg agcugcaccg gaacggccug augacgcagc gcaagcucaa gcaccuccug     2760

augcggccgg acgagaucuc caagcacgcg accgacuuca ucaaccgcca gcugguggag     2820

acccgccagg ugaucaagcu ggucgaggag cugaucagcu ccgaguaccc cagcgccucc     2880

aucguggccg ugaaggccaa ccucacccac caguuccggc agacguucaa cuuccccaag     2940

cugcgcgagg ucaacgacua ccaccacgcc uucgacgcga gccugaccgc cuucaucggc     3000

auguaccucc ugaagcagua cccgaagcug gagcgguucu ucguguacgg gaaguucgcc     3060

aagcagccca ucaaccucac ccgcuucaac gugauccgga agcuggccgu cgcggaggag     3120

cccaucgcca acaucgagac cggcgagauc cugugggaca agacggccga caucaagugc     3180

uucgagaagc ucuacaacua caagcgccug cuggugaccc acgaggugcg ggagaacuac     3240

ggggccaugu ucgaccagac caucuucaag gccaccgacu cccgcaagcg gcucauccag     3300

aagaagaagg gcuucgcgac ggagaccuac gggggcuaca ccggccgcac ccuggccuac     3360

cuggccaucg ucaagguggu gacgaacaag gacacccagu accaggucgu ggggaucccc     3420

accaccaugg ugacgcagau ccagcagcuc gaggccagcg gccugaccuc caagcaggcc     3480

gucaccaagg cgaucggggc caugaucaac gagaacaaca agaagcugca gagcuacgag     3540

gugcuccugc cgaaggugcg guucgagcag gucgugcgcg acgaggugaa gggccacauc     3600

caccgguucg cccuggggac cgacacguac uaccacaaca uccaggagcu cuaccugccc     3660

cugaccaucc agcgcgccuu ccucggcaag cggaacgaca ccgacgccca gaccgagcag     3720

aaccugcuga cggucuucga gaccacccuc gcccagguga accgcuacuu cccccuguac     3780

gagaugaaca aguucaagca gauccugaac uccgcgcagg agaaguucgc cgcccucccc     3840

accaagaaca agaccgugaa gaacaagcug gucacgggca aggacggggu gcugaacaac     3900

cucuucaucg gccugcacgc caacgcgagc cugggggacc ucaagauccu gggcaugaag     3960

accggguucg gcaagcucca ggugccgggc gggcugaagc ugaccgacca ggccgagauc     4020

aucuaccaga gccccaccgg ccuguucgag cggaaggucg cccucaagga ccuguga        4077


<210>  1895
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1895
augaacgagc ccuacggcgu ggggcuggac aucggcacca acagcgucgg guggaccgug       60

guggacauga acggccgcgu ccggaaggug aaggggaaga cggcccucgg cgcccgccug      120

uucaaggagg gcgcgaccgc cgaggaccgg cgcggguucc ggaccacccg ccggcgccug      180

aagcggguga aguggcgccu ccggcugcug cgcgaguucu ucgaccagcc gaucuccaag      240

aucgacccca acuucuucgc ccggcgcaag uacagcgaca ucagcccccg ggaccccaac      300

uacaacggcc ucgccaagac gcuguucaac gaccgcaccg accaggaguu cuaccacgac      360

uacccgacca ucuaccaccu ccgggacaag cugaugaccu ccgaccgcaa guucgacauc      420

cgggagaucu accuggccau ccaccacauc gucaaguacc gcgggaacuu ccugcggacg      480

ggccccgcgu cccaguacgg gagcgccucc cuccaccugg ccaccagcuu ccagaagcug      540

aacgaccucu ucgcccaguc cgaggagagc cugaacuuca agcuggugac cgacgaggcg      600

cagcuccagc agauccagca gauccuggug cgcaccgacc ugucccgcag cgagcagcag      660

cggcagaucu ggccccucau ggucgugcug augggcgcca cggccgccga gaagaagcgc      720

cagaaggacu ucgccaagga gcuggcgaac gcccucgugg gccugaaggc caagcugaac      780

gucgugaccc ucaccaacgu ggacaccacg gucgugaagg acuggaccuu cacccuggag      840

gagucccagg acaagcugcc cgagaucgag gagcagcuca gcgagguggg gcagcagauc      900

auggacgagg ucauccagcu guacgccucc gugaaccugg cccagcucau cccggcgggc      960

aagcgguuca gccagcacau gguggagaag uacaaccacc acgagaagaa ccuggagcug     1020

cucaaggccu acauccacuc ccagagcgac uccaagcgcg ggcgggagau ccgcgccacc     1080

uacgaccggu acaucgacgg cgucaagagc aagcccguga cgcaggagau guucuacaag     1140

gaccugauga aguacgugga ggccgacgcc accagcaacc accuggcggc cgagaucaag     1200

gacgagaucg acuccgagca guucaugccc aagcuccgca ccaagcagaa cggcagcauc     1260

cccuaccagg uccagcagua cgagcuggac cagaucaucu cccaccagaa gaaguacuac     1320

ccguggcugg gggaggagaa ccccguggcc gagcggcgcg gcaaguuccc cuacaagcuc     1380

gacgagcugg ucggguuccg ggugcccuac uacgugggcc cgcugaucac caaggaggac     1440

cagcaggcca cgagcggggc cggcuucgcg uggauggucc gcaaggccga cggccccauc     1500

acccccugga acuucgacca gaagguggac cggaucgccu ccgccaccgc guucauccag     1560

cgcaugcaga ccacggacac cuaccucauc ggggaggacg ugcugcccgc ccggagccug     1620

aucuaccagc gcuucauggu ccucaacgag cugaacaaca ugcgggugga ggaccagaag     1680

cuggccccgc gccagaagca gcggcuguac aaccaggugu ucaagcagca ccagcacguc     1740

uccgugaaga acauccagca gaaccucaug gacgccggcg aguaccgcga gacccccaag     1800

aucaccgggc uggccgaccc caagggcuuc aacagcuccc ugagcacgua ccacgacuuc     1860

aagaagaucc uccccgaggc gaucgccgac gagcacaagc gcgccgacau cgagaagauc     1920

auccuguggu ccaccaccuu cgaggacagc gccaucuuca agcagaagcu ggaggaggug     1980

gccuggcuca ccgacgcgca gcggaagcag cuguccgggc ugcgcuaccg gggcuggggc     2040

cagcucagcc acaagcugcu gacggccuuc aaggacgaca acgggcgcuc cgucauggac     2100

ggccucuggg agaccagcga caacuucaug cagcugcgga agcagccggc caucgagacc     2160

cagaucaagg aggccaacca ggccaaccug accgacgcgg acauccagga caccaucaac     2220

gagcucuaca cgagccccca gaacaagaag gccauccgcg aggugaugcu ggugcuggac     2280

gacaucaaga acgccaugca cgggcagacc cccuccugga ucuucaucga ggccgcgcgg     2340

ggcgggggcg ucgccggccg ccggacccag ucccgcagca agcagaucgu ggaggccuac     2400

aagagcaccg ccaaggagau cguguccgag aagguccagc acgagcucaa cgagaagauc     2460

aaggccaagg cggacuucaa cacgcggcug gugcuguacu uccuccagaa cgggcgcgac     2520

cuguacacca acgaggccau caacaucgac cggcugagcg aguacgacau cgaccacauc     2580

cucccccagu cccuggugaa ggacgacagc cuggacaacc gcguccucac cuccgcccgg     2640

aucaaccgcg agaagaacga caccuucgcc agcgagaagu ucggccggaa gaugggggcc     2700

caguggcgcg agcugcaccg gaacggccug augacgcagc gcaagcucaa gcaccuccug     2760

augcggccgg acgagaucuc caagcacgcg accgacuuca ucaaccgcca gcugguggag     2820

acccgccagg ugaucaagcu ggucgaggag cugaucagcu ccgaguaccc cagcgccucc     2880

aucguggccg ugaaggccaa ccucacccac caguuccggc agacguucaa cuuccccaag     2940

cugcgcgagg ucaacgacua ccaccacgcc uucgacgcga gccugaccgc cuucaucggc     3000

auguaccucc ugaagcagua cccgaagcug gagcgguucu ucguguacgg gaaguucgcc     3060

aagcagccca ucaaccucac ccgcuucaac gugauccgga agcuggccgu cgcggaggag     3120

cccaucgcca acaucgagac cggcgagauc cugugggaca agacggccga caucaagugc     3180

uucgagaagc ucuacaacua caagcgccug cuggugaccc acgaggugcg ggagaacuac     3240

ggggccaugu ucgaccagac caucuucaag gccaccgacu cccgcaagcg gcucauccag     3300

aagaagaagg gcuucgcgac ggagaccuac gggggcuaca ccggccgcac ccuggccuac     3360

cuggccaucg ucaagguggu gacgaacaag gacacccagu accaggucgu ggggaucccc     3420

accaccaugg ugacgcagau ccagcagcuc gaggccagcg gccugaccuc caagcaggcc     3480

gucaccaagg cgaucggggc caugaucaac gagaacaaca agaagcugca gagcuacgag     3540

gugcuccugc cgaaggugcg guucgagcag gucgugcgcg acgaggugaa gggccacauc     3600

caccgguucg cccuggggac cgacacguac uaccacaaca uccaggagcu cuaccugccc     3660

cugaccaucc agcgcgccuu ccucggcaag cggaacgaca ccgacgccca gaccgagcag     3720

aaccugcuga cggucuucga gaccacccuc gcccagguga accgcuacuu cccccuguac     3780

gagaugaaca aguucaagca gauccugaac uccgcgcagg agaaguucgc cgcccucccc     3840

accaagaaca agaccgugaa gaacaagcug gucacgggca aggacggggu gcugaacaac     3900

cucuucaucg gccugcacgc caacgcgagc cugggggacc ucaagauccu gggcaugaag     3960

aucggguucg gcaagcucca ggugccgggc gggcugaagc ugaccgacca ggccgagauc     4020

aucuaccaga gccccaccgg ccuguucgag cggaaggucg cccucaagga ccuguga        4077


<210>  1896
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1896
augaaggagc ccuacggcgu ggggcuggac aucggcacca acagcgucgg guggaccgug       60

guggacgccu ccggccacgu ccgcaagauc aaggggcaga cgggcaucgg cgugcggcug      120

uucaaggagg gggccgcggc cgccgaccgc cggggcuucc gcaccacccg gcgccggcug      180

aagcgcguga aguggcggcu gcgccuccug cgggaguucu ucgaccagcc gaucagcaag      240

gucgacauca acuucuucgc ccgccggaag uacuccgaca ucagcccccg cgaccccaac      300

uacaacgggc uggagaagac ccucuucaac gaccgguccg accaggacuu cuaccacgac      360

uaccccacga ucuaccaccu ccgcgaggcc cugaugaccc agcaccggaa guucgacgug      420

cgcgagaucu accucgcgau ccaccacauc gugaaguacc ggggcaacuu ccugcgcaac      480

gacgccgcca ccgccuaccg gagcgggacc cuggaccucc agcagcacuu cgagacgcug      540

aaccaccugu ucucccaggc ggaccucgag cugaaccuga accucaccac cgacgucgcc      600

cugcuggaca gcaucaagca gacccucgug cgcacggaca ucucccgcag cgaccggcag      660

aagcugauca ugccgcugcu cgccgugcug accggcgcca ccaccgccga gaagaagcgc      720

cagaaggcgg ucgugacgga guucgccaag gcccuggugg gcaacaagac caagaucgac      780

guccucaccc ugaccgacau cgacgccacg gaggccaagg acugggcguu cucccuggag      840

gagaaccagg acaagcuccc cgggaucgag gaccgguuca gcgagguggg ccagcagauc      900

aucgacgagg ugauccgccu guacgccucc gucaaccugg cccagcucau ccccgagggg      960

aagcgguuca gccaguccau gguggagaag uacaagcgcc acggcgagga ccugaagcug     1020

cucaaggccu acauccggag ccaguccgac gccaagcgcg gccgggcgau ccgcgccacc     1080

uacgaccagu acaucgacgg ggugaagucc aagcagguca cccaggaggc cuuccagaag     1140

gcccugggca aguucgugga cgccgacgug gaccugaacu ccugggcggc ccagaucaag     1200

caggccaucg acagcgagca guucaugccc aagcuccgga ccaagcagaa cggggccauc     1260

ccguaccagg uccagcagaa cgagcuggac cagaucauca acaaccagaa ggaguacuac     1320

cccuggcugg gcgaggagaa ccccgugauc aagcgccggg ggaaguuccc cuacaagcuc     1380

gacgagcugg ucggcuuccg cgugccguac uacgugggcc cccugaucac gaaggagaug     1440

caggcggcca ccgccaacgc cggguucgcc uggauggucc ggaaggcgga cggccccauc     1500

acccccugga acuucgacga caagguggac cgcguggcca gcgccaccgc cuucauccag     1560

cggaugcaga cgaccgacac cuaccucauc ggggaggagg uccugccggc ccgcucccug     1620

aucuaccagc gguucauggu gcucaacgag cugaacaaca uccgcgugga cgacggccag     1680

cugacgcccc gccagaagca gcggcuguac aaccaggucu ucaagaagca caagcgcgug     1740

accgcgaaga acauccagga caaccucgug gccgccgggg aguuccccac cgcgccccgg     1800

aucaccggcc uggccgaccc gaaggucuuc aacagcuccc ugagcaccua ccacgaccuc     1860

aagcaggucc ugcccgaggc cauguccgac gugcgcaagc aggccgacau cgagaagauc     1920

auccugugga gcacgaucuu cgaggacucc accaucuuca agcagaagcu caccgagauc     1980

agcuggcuga ccgccgccca gcugaagcag cucggccagc ugcgguaccg cggguggggc     2040

cagcuguccc ggaagcuccu gaccgggcug aaggacgaca acaaccgcag caucauggac     2100

ggccucuggg agacgaacgc gaacuucaug cagcugcggu cccagcccgc caucgagaac     2160

cagaucaaga gcgccaacca ggccaaccug accaccgccg accuccagga caccaucaac     2220

gagcuguaca cgagccccca gaacaagaag gcgauccgcg aggugauggu ggugcuggcc     2280

gacaucaagg acgccaugca cgggcagacc ccgaacugga ucuucgucga ggccgcccgg     2340

ggcggcgggg uggcgggccg ccggacccag agccgcucca agcagaucgu ggccgccuac     2400

caggggaccg cccaggucau cgugagcgac caggugcagc gggagcucaa ggagaagauc     2460

aagaacaagg ccaacuucaa cacgcgccug guccuguacu uccuccagaa cggccgggac     2520

cuguacaccg gcgacgcgau caacaucgac cgccuguccg aguacgacau cgaccacauc     2580

cucccccaga gccuggugaa ggacgacucc cuggacaacc gggugcucac caacgcccgc     2640

aucaaccggg agaagaacga caccuucgcc agcgagaagu ucgggaagaa gaugggcgcc     2700

aaguggaagg agaugcaccg cauggggcug augacgaagc gcaagcugga ccaccuccug     2760

augcggcccg acgagaucag caaguacgcg accggcuuca ucaaccgcca gcuggucgag     2820

acccgccagg ugaucaagcu cguggaggag cugaucaacg ccgaguaccc cuccaccgcc     2880

aucgucagcg ugaaggccaa ccugacgcac caguuccggc agaccuucga cuucccgaag     2940

auccgggagg ugaacgacua ccaccacgcc uucgacgcgg cccucaccgc cuucaucggg     3000

accuaccugc ugaagcgcua ccccaagcuc gagcgguucu ucgucuacgg caaguucgcc     3060

aagcagcccg ugaaccugac gcgcuucaac gugauccgga agcuggccaa gaccgacacc     3120

ccgaucaccg acaucgacac gggcgagguc cucugggaca agcugaccga caucaaguac     3180

uucgagaccc uguacgacua caagcgccuc cuggugaccc gggagguguc cgagaacuac     3240

ggggcgaugu ucgaccagac gcugcucaag gccagcgaca acggcaagcg ccugauccgg     3300

aagaagcagu ccuucgacac cgccaucuac gggggcuaca ccgggaagac ccuggccuac     3360

auggccaucg ucaaggugag cacgaagaag gacgugcagu accaggucgu gggcgugccc     3420

accaccaugg ucacccagau cgagcagcuc gagcagcagg gcaugacgaa caagcaggcg     3480

gugaccaagg ugaucgagcc ccugaucgcc gccaacaaca agaagaugcu gaccuacgag     3540

guccuccugc ccaaggugcg cuucgagcag gucgugcggg accgcaucaa ggggcagacc     3600

caccgguucg cccugggcac cgacacguac uaccacaaca uccaggagcu cuaccugccg     3660

cugacccucc agcgcgccuu ccuggggaag cagaacgagu ccgaggugca gcugaaccag     3720

aaccuccuga ccgucuucga cgcgacccug gcccagguga accgguacuu cccccucuac     3780

gagaugaaca aguucaagaa gauccugaac agcgcccgcg accagaucga gcugcucgag     3840

gccaagaacc aguucgugaa gaacaagcug guccugggca agcaggggau ccucacgaac     3900

auguucaucg gccugcacgc gaacgccucc uucggcgacc ucaagagccu gggggugaag     3960

accuccuucg gccagcucca ggugcccggg ggcaucaagc ugaccgggga cgccgagauc     4020

aucuaccaga gccccaccgg ccuguucgag cgcaagaucg cccugaagga ccuguga        4077


<210>  1897
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1897
augaagcagg gcaagcgcgg guacuaccug ggccucgaca ucgggaccga cagcaucggc       60

uacgccguga ccgacgagca guaccaccuc cugcgguucc acgggaacga cgccuggggc      120

ucccacgucu ucgacagcgc gucccucugc gacgagcgcc ggggcuuccg cagcgcccgg      180

cgccggcugg accgccggca gcagcgcgug aagcugcucc aggagaucuu cgccaaggag      240

aucgccaaca ucgacccccg guucuucauc cgccuguccg agagcuaccg guggcgcgac      300

gacacggacg accgguacau cuacuucaac gaggaggagu acaccgacau ccaguacaug      360

cgcgacuacc cgaccaucca ccaccugauc ugcgagcuca uggagaacga ggaggagcac      420

gacauccggc ugguguaccu ggccugcgcg uggcucgucg cccaccgcgg gcacuuccug      480

ucccagcuga gcguggacaa caucgacucc cucaucgaca ucgagagcgu guaccagaag      540

uuccugcacu ucuucaucga caacggcugc gagaaccccu ggauccagga cgccgagcug      600

gucgggggca uccucaagga gcacaucggg gugaccgaga agcacaaccg gcugggcgag      660

aucaugcugg gcaaccgcaa gcccuccaag gacgugcggg acacguuccu cuacagcgag      720

uacugcauca uccgccugcu ggccgggggc acgugcaagc uccgcgaccu guacgggaag      780

gaggaguacg aggacuucgg cuccgucagc cuggagaugg acgacgacaa gcucgcggag      840

aucaugauca acaucggcga ggacuacgag cugaucgccg ugcugcgguc ccuccacgac      900

uggagcgugc uggccgacau ccuggacggg cagaacggcc ucaccaacau cuccaccgcc      960

aaggucgcca ucuacgagca gcacaagaag gaccuggcga cgcugaagua cuucauccgc     1020

aaguacugcc ccgagaagua caacgccauc uucaaggacg cccgggagga caacuacgug     1080

gccuacagcu accacuacaa caacaagcuc gacgggcaga ucaagaagaa ggccgacacc     1140

gagaccuucu ccaaguuccu gaucaagauc auccacagca ucaagccgga caaggaggac     1200

gagaucaucu accaggacau ggacaaccgc cugggccucc ggcaguuccu gcccaagcag     1260

cgcaacaccg acaaccgggu gaucccccac cagcuguacg aguacgagcu caagaagauc     1320

cuggagaacg cggccaagua ccugagcuuc cucaaccaga aggaggacgg gaucuccaac     1380

acggagaaga ucaucagcau cuucaaguuc aagauccccu acuacgucgg cccgcugaac     1440

aagcacuccg aguucgccug gaucgaccgc aagagcggca agauccugcc cuggaacuac     1500

gagaacauga ucgacgacga cgccuccgag gaggccuuca ucaagaagau gaccaaccag     1560

ugcaccuacc uccccgggga gagcgugcug cccaaggacu cccugugcua ccagaaguuc     1620

auggugcuga acgagaucaa caacaucaag gucgacggcc ggaagaucag cgugggggcg     1680

aagcagggca ucuacuccga ccucuuccug aaguacaaga aggugaagcg cagccagauc     1740

gaggaguacc ugaucuccaa cgggcagcuc gagaagaacc ggcgcgagac ccugagcggc     1800

aucgacaucc agaucaaguc cagccugucc agccacaucg ccuuccggcg ccuccuggag     1860

cagaagaucc ugaacgaggc cgacgucgag cggaucaucg agcgcgccuc cuacgccgag     1920

gacaagggcc ggguggcgaa guggcuccgc gccaaguacc cgcaccugac ggaggccgac     1980

aucaaguaca ucugcaaggu gaagaucaag gacuucgggc ggcugagccg caccuuccuc     2040

accggcaucg agggggccug caaggagacc ggcgaggucg cgacgaucau cuccaugaug     2100

ugggagagca acgacaaccu gauggagcug cucagcgagc gcuacaccuu cgccgacacc     2160

auccuggagu ucaaguccga cuacuacacc gagcggaagc agacgcugag cgagcgccuc     2220

gacgagaugu acguguccaa cgccguccgg cgccccaucu accggacccu ggacaucgug     2280

aaggaccugg agaaggccuu cgggaagccc gagaagaucu ucaucgagau gacccgcggc     2340

gccaugcccg agcucaaggg caagcggacc aagagccgcc agcagcagcu gcuggaguac     2400

uacgacaagu gcaaggagga ggacgugcgg gaccucaagc agcagcugga ggcgcugggg     2460

gaguacgucg acaacaagcu ccaguccgac cgccuguucc uguacuucau gcaguucggc     2520

aagugcgccu acagcgggau gccgaucucc cucgagcggc ugauggccgg cagcaaggag     2580

uacgacaucg accacaucua cccccaggcc uacgugaagg acgacuccau caucaacaac     2640

aaggugcugg uccucagcgu ggccaacggc gagaagaagg acguguaccc caucaagucc     2700

gagauccgca acaagaugca gaagacgugg agcuucuggc accacgucgg gaccaucucc     2760

gacgagaagu acaagcggcu gauccgcagc acccccuuca ccgaggacga gaaguacggc     2820

uucaucaacc ggcagcugac ggagaccucc cagagcacca aggugguggc ggagcuccug     2880

aaggagcgcu acccggaggc cgagaucguc uacuccaagg ccgggcugau cagcgacuuc     2940

cggcacgagu ucgaccucua caagagccgc uccuacaacg accugcacca cgccguggac     3000

gccuaccuga acaucguggu cggcaacgug uaccacaugc gguucagccg ccagugguuc     3060

aacgugaacu ccagcuacuc caucaagacc aagacccucu ucacccacac cgucaacugc     3120

aacgggaagg agguguggaa caaggacaug cugcccggcg ugcugaagac cgcgaagaag     3180

aacaccgccc acuucacgaa gcacgccacc uucaagaccg gcgggcuguu cgaccagaac     3240

cccgucaaga aggcccccgg ccugaccccg cugaaggcgg ggcuccccac ggagaaguac     3300

ggcggguaca acaaggccgg cgugauguuc uucauccccg ugcgguacaa gagcggcaag     3360

aagaccgucc ugauggugcu guccguggag cuccuguacg ggaaccgcuu ccuggaggac     3420

gagaucuucg ccaaggagua cgccaaggac cgccuccagc ggaugaucgg caagagcguc     3480

gacgagaucg acuuccccau cgggaugcgc ccguggaagg ugaacaccau ccugucccug     3540

gacggcuucc ggaucugcau caccgggaac gccagcggcg gcaagugccu caucgcgcag     3600

cccaucaugc aguucuccag cgacgaguac uggaaguacu accugaagaa gguggagaag     3660

uucgucgaga aggugaagaa caacuccagc uacguguacg acgucgacua cgacgccgug     3720

cagcacgagg acaaccugaa gcucuacgac cuguaccugg acaagcucca gaacuccauc     3780

uaccgcaagc ggaucaacgc ccccauccag acgcugaucg aggggcgcga gaaguucauc     3840

gacugcagcg ugaucgagca gugccagguc cugcucaaca uccaccaggu guucggccgg     3900

augaccuccg ggugcgaccu gacccugauc ggcgggaaga cccgcagcgc cuccacggug     3960

aacuucagca gcaucauccu caacuggaag aagaacuaca ccgacguccg gaucaucgac     4020

caguccgcca gcggccugug ggagguggug uccgagaaca uccuggagua ccucuga        4077


<210>  1898
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1898
augggcaaga uguacuaccu ggggcucgac aucggcacca acagcguggg guacgccguc       60

accgaccccu ccuaccaccu ccugaaguuc aagggcgagc cgaugugggg cgcccacgug      120

uucgcggccg ggaaccagag cgccgagcgc cgguccuucc gcacgagccg gcgccggcuc      180

gaccgccggc agcagcgcgu gaagcugguc caggagaucu ucgcccccgu gaucuccccc      240

aucgaccccc gguucuucau ccgccugcac gagagcgccc ucuggcggga cgacguggcg      300

gagaccgaca agcacaucuu cuucaacgac ccgaccuaca ccgacaagga guacuacucc      360

gacuacccca cgauccacca ccugaucguc gaccugaugg agagcuccga gaagcacgac      420

ccccgccucg uguaccuggc cguggccugg cuggucgccc accggggcca cuuccucaac      480

gagguggaca aggacaacau cggggacgug cugagcuucg acgcguucua ccccgaguuc      540

cuggccuucc ucuccgacaa cggcgucagc ccgugggugu gcgaguccaa ggcccugcag      600

gccacccugc ucagccgcaa cuccgugaac gacaaguaca aggcccucaa gagccugauc      660

uucggguccc agaagcccga ggacaacuuc gacgcgaaca ucagcgagga cggccugauc      720

cagcugcugg ccgggaagaa ggucaaggug aacaagcugu ucccccagga guccaacgac      780

gccagcuuca cccugaacga caaggaggac gccaucgagg agauccuggg cacccucacg      840

cccgacgagu gcgaguggau cgcccacauc cggcgccugu ucgacugggc gaucaugaag      900

cacgcccuga aggacggccg gaccaucagc gaguccaagg ugaagcucua cgagcagcac      960

caccacgacc ugacccagcu gaaguacuuc gucaagaccu accucgccaa ggaguacgac     1020

gacaucuucc gcaacgugga cagcgagacg accaagaacu acguggccua cuccuaccac     1080

gucaaggagg ugaaggggac ccugccgaag aacaaggcca cccaggagga guucugcaag     1140

uacgugcugg gcaaggucaa gaacaucgag ugcagcgagg cggacaaggu ggacuucgac     1200

gagaugaucc agcggcucac ggacaacucc uucaugccca agcaggucag cggggagaac     1260

cgcgugaucc ccuaccagcu guacuacuac gagcugaaga ccauccucaa caaggccgcc     1320

uccuaccugc ccuuccugac ccagugcggc aaggacgcca ucagcaacca ggacaagcuc     1380

cuguccauca ugaccuuccg gaucccguac uucgugggcc cccugcgcaa ggacaacagc     1440

gagcacgccu ggcucgagcg caaggcgggg aagaucuacc ccuggaacuu caacgacaag     1500

gucgaccugg acaaguccga ggaggccuuc auccggcgca ugacgaacac gugcaccuac     1560

uaccccggcg aggacgugcu gccgcucgac agccugaucu acgagaaguu caugauccug     1620

aacgagauca acaacauccg gaucgacggg uaccccaucu ccguggacgu caagcagcag     1680

gucuucggcc uguucgagaa gaagcgccgg gugaccguga aggacaucca gaaccuccug     1740

cugagccucg gggcccugga caagcacggc aagcugaccg gcaucgacac caccauccac     1800

uccaacuaca acaccuacca ccacuucaag agccucaugg agcgcggggu gcugacgcgg     1860

gacgacgugg agcgcaucgu cgagcggaug accuacuccg acgacaccaa gcgcgugcgg     1920

cuguggcuca acaacaacua cggcacccug acggccgacg acgugaagca caucagccgc     1980

cugcggaagc acgacuucgg gcgccucucc aagauguucc ugaccggccu gaaggggguc     2040

cacaaggaga ccggcgagcg ggcgagcauc cucgacuuca uguggaacac caacgacaac     2100

cugaugcagc ugcucagcga gugcuacacg uucuccgacg agaucaccaa gcugcaggag     2160

gccuacuacg ccaaggccca gcugagccuc aacgacuucc uggacuccau guacaucagc     2220

aacgccguga agcgccccau cuaccggacc cuggcggugg ucaacgacau ccgcaaggcc     2280

ugcggcaccg cccccaagcg gaucuucauc gagauggccc gcgacgggga guccaagaag     2340

aagcgcagcg ugacccggcg cgagcagauc aagaaccucu accgguccau ccgcaaggac     2400

uuccagcagg agguggacuu ccuggagaag auccuggaga acaagagcga cggccagcuc     2460

caguccgacg cccuguaccu guacuucgcg cagcucgggc gggacaugua cacgggcgac     2520

ccgaucaagc uggagcacau caaggaccag agcuucuaca acaucgacca caucuacccc     2580

caguccaugg ucaaggacga cagccuggac aacaaggugc ucgugcaguc cgagaucaac     2640

ggggagaagu ccagccgcua cccccuggac gccgccaucc ggaacaagau gaagccccug     2700

ugggacgccu acuacaacca cggccucauc agccugaaga aguaccagcg ccugacccgg     2760

uccaccccgu ucaccgacga cgagaagugg gacuucauca accgccagcu cgucgagacg     2820

cggcagagca ccaaggcccu ggcgauccug cucaagcgca aguuccccga caccgagauc     2880

guguacagca aggccggccu guccagcgac uuccggcacg aguucgggcu ggugaagucc     2940

cgcaacauca acgaccucca ccacgccaag gacgccuucc uggcgaucgu caccggcaac     3000

guguaccacg agcgguucaa ccgccggugg uucaugguga accagcccua cagcgucaag     3060

acgaagaccc uguucaccca cuccaucaag aacgggaacu ucguggccug gaacggcgag     3120

gaggaccucg gccgcaucgu gaagaugcug aagcagaaca agaacaccau ccacuucacg     3180

cgguucagcu ucgaccgcaa ggaggggcug uucgacaucc agccgcucaa ggccuccacc     3240

ggccuggucc cccggaaggc cgggcuggac guggugaagu acggcgggua cgacaagagc     3300

accgccgcgu acuaccuccu gguccgcuuc acccuggagg acaagaagac gcagcacaag     3360

cucaugauga uccccgugga gggccuguac aaggcccgca ucgaccacga caaggaguuc     3420

cugaccgacu acgcccagac caccaucucc gagauccucc agaaggacaa gcagaaggug     3480

aucaacauca uguuccccau gggcacgcgg cacaucaagc ugaacagcau gaucuccauc     3540

gacggguucu accugagcau cggcgggaag uccagcaagg gcaaguccgu ccucugccac     3600

gccauggugc cgcugaucgu gccccacaag aucgagugcu acaucaaggc cauggagagc     3660

uucgcgcgca aguucaagga gaacaacaag cugcggaucg ucgagaaguu cgacaagauc     3720

accguggagg acaaccucaa ccuguacgag cuguuccucc agaagcugca gcacaacccc     3780

uacaacaagu ucuucuccac ccaguucgac gugcugacca acgggcgcag cacguucacc     3840

aagcucuccc ccgaggagca gguccagacc cugcugaaca uccucagcau cuucaagacc     3900

ugccggagcu ccggcugcga ccucaagagc aucaacggcu ccgcccaggc cgcccgcauc     3960

augaucagcg ccgaccugac ggggcugucc aagaaguaca gcgacauccg gcugguggag     4020

caguccgcga gcggccuguu cguguccaag agccagaacc uccuggagua ccuguga        4077


<210>  1899
<211>  4083
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1899
augaccaagc ugggcaagcc cuacgggauc ggcguggaca ucgggagcaa cuccaucggc       60

uucgccgccg ucgacgagaa cagccaccuc auccgccuga aggggaagac cgugaucggc      120

gcgcggcugu ucgaggaggg caaggccgcc gccgaccgcc gggccucccg cacgacccgg      180

cgccggcuca gccgcaaccg guggcgccug uccuuccugc gggacuucuu cgagagccac      240

aucaccccga ccgaccccaa cuucuucaug cgccagaagu acuccgagau cagccccaag      300

gacaaggcgc gguacaagua cgagaagcgc cucuucaacg accggacgga cgccgaguuc      360

uaccagcagu accccaccau guaccaccuc cgcacccggc ugcugaccga cccguccaag      420

gccgacgugc gcgagaucua cuucgccauc caccacaucc ucaagagccg ggggcacuuc      480

cugacguccg gcuacgcgaa ggacuucaac accaacaagg ucgcccugaa cgagaucuuc      540

cccgcccugc aggacgccua cgcccaggug uaccccgacc uggacaucac cuucgacgag      600

aacaagguga acgaguucaa gaccguccuc cuggacgaga aggcgacgcc cagcgacacc      660

cagcgcgccc uggugaaccu ccugcuggcc gaggacgggg acaaggacau ccucaagcag      720

cagaagcagg uccugaccga guucgccaag gccguggugg gccugaagac caagcucaac      780

guggcgcugg ggacggaggu cgacuccagc gaggccaccg ccuggaacuu cucccugggc      840

cagcucgacg acaagugggc cggcaucgag agcgccauga ccgacgaggg gaccgagauc      900

cuggaccaga uccgggaccu guaccgcgcg cgccuccuga acggcaucgu gccggccggg      960

aagacgcugu cccaggccaa gguggacgac uacgagcagc accacgagga ccucgagcug     1020

uucaaggacu accugaagca gcucgaggac gacggcaugg ccaaggccau ccggcagcug     1080

uacgaccgcu acaucgacgg cgacgacgcg aagcccuucg uccgggagga cuucgugaag     1140

gcccugacca aggaggugac cgcccacccc aacgccaaga gccccgagcu ccuggagcgc     1200

cugguccagc cggacuucau gcucaagcag cggaacaagg cgaacggggc cauccccgug     1260

cagaugcagc agcgcgagcu ggaccagauc aucaagaacc aguccgugua cuacgacugg     1320

cuggccgccc ccaaccccgu cgagaagcac cggcagagca ugccguacca guucgacgag     1380

cuccugaacu uccgcauccc cuacuacgug ggcccccugg ugaccgccaa ggagcagaag     1440

gcggcccagg gcggggucuu cgccuggaug gugcggaagg accccgacgg gaacaucacg     1500

ccguacaacu ucgacgagaa ggucgaccgc gaggccagcg ccaacaccuu cauccagcgg     1560

augaagacca ccgacacgua ccucaucggc gaggacgugc ugcccaagca gucccugcug     1620

uaccagagcu acgaggugcu caacgagcug aacaacgucc gcgugaacga caacaagcug     1680

uccuucgacc agaagcagca gguccucggc gagcuguucg agcggcacaa caccgugacc     1740

aucaagcagu ucguggacaa ccugcucgcg cacggggacu acgaccgcau ccccgaggug     1800

cggggccugg ccgacgagaa gcgcuuccug agcucccuca gcaccuacaa ccagcugaag     1860

gggauccugc acgaggccau cgacgacucc gacaagcaga cggacaucga gaagaucauc     1920

gccuggagca ccaucuucga ggacaaggcc aucuucaaga ccaagcucaa cgagaucucc     1980

uggcugaacg cgggcaugau caacagccug gccaacaucc gguaccgcgg guggggccag     2040

uucucccaca agcuccugaa cgagcugacc cucagcacgg gccggaccgu cuucgaggag     2100

cugcugaucu ccagccacaa ccucaugcag auccugaccg acgaggugcu gaagcagaag     2160

aucgccgagc ucaacgccca cgagcugaag gcguccggga ucuacgccgc caucgacgac     2220

gccuacacca gccccuccaa caagaaggcc cugcgccagg ugcucagcgu caucgacgac     2280

aucaccaacg cggccggcca ggccccgaac uggcuguaca ucgagacggc cgacggggac     2340

ggcgugcccg ggaagcgcac caacucccgg cagcgcaaga uccaggagau cuacgugaac     2400

gccgcgcagg agcugaucac cggcagcguc cggggcgagc ucgaggacaa gaucgccagc     2460

cgcgccgacu ucaccgaccg gcuggugcug uacuucaugc agggcgggcg cgacaucuac     2520

acgggggagc cccucaacau cgacaaccug uccagcuacg acaucgacca cauccugccc     2580

cagucccuca ucaaggacaa cagccuggac aaccgggugc ugguccaccc gcucgugaac     2640

cgcgagaagg acaccaucuu cgccuccacc cgguucgcca agcagaugaa cggcaugugg     2700

cgccaguggc acgcggccgg ccugaucagc ucccggaagc ugcgcaaccu ccagaugcgg     2760

cccgacgaga ucgacaagua cgccagcggg uucguggccc gccagcuggu cgagacccgg     2820

cagaucauca agcugacgga gcagaucgug gcggaccagu accccgacgc caagaucauc     2880

gccgugaagg ccggccucuc cagccagcug cgcaaggagc uggacuuccc gaagaaccgg     2940

gaggucaacc acuaccacca cgccuucgac gcguuccucg ccgcccgcau cgggaccuac     3000

cugcugaagc gguaccccaa ccucgagccc uucuucaccu acggcaaguu caagaagacc     3060

gaggugaaga agcugaaguc cuucaacuuc auccgcgaca ugacgcacgc caaggacaag     3120

aucguggcca aggagaccgg ggagaucguc ugggacaacg cgagcgacau caacgagcug     3180

gaccggaucu acaacuucaa gcgcaugcuc aucacccacg aggugcgcuu cgagaccgcc     3240

ucccuguuca agcagacgcu guacgccgcc aagaacagca agaaccgggg cggcucccgc     3300

cagcucaucc ccaagaagaa gggguaccug guggacaucu acggcgggua cacccaggag     3360

accggcagcu accuguccgu cgugcggcuc accaagaagg ccauguacgc gguggucaag     3420

gugagcacgc gcgacgccgc caagcuggcc guggccaaga gcaucuccga gcagaaggag     3480

aacgagaccc ugaagaagau caucgacggg aagcucagca agaccuccaa gaagggcaag     3540

accacgcacc agcuguucga gaucguccug ccgcgggugg gccagaagac ccucuucaag     3600

aacagcaagu acaaccaguu ccuggugaac uccgacaccu acaugcacaa cuaccaggag     3660

cuguggaugc cccgcgagua ccagcggaug uggaaggaca uccuccugag caaccacggg     3720

gacgcgcagc uggagggcca gcucgaccag aucuucaagu ucaucgucuc ccaggugaac     3780

agcuacuuca accuguacga caucaaccag uuccgcaaga agaucgccga cgccaccgac     3840

aaguucgcca agcugcccac gcacgacacc gacaacgcgc gggggaagau cgccaccauc     3900

ggccagcucc ugaucgggcu gcaggccaac gccaugcgcu ccgaccuccg gaaccuggac     3960

aucaagaccc ccuucggccu gcuccagauc ggcacgggga ucacccugga ccuggacacc     4020

agcaucaucu accagucccc gaccggccuc uucgagcgcg aggugccccu gacggaccug     4080

uga                                                                   4083


<210>  1900
<211>  4083
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1900
augaccaagc ugaaccagcc cuacggcauc gggcucgaca ucggcagcuc cagcaucggg       60

uucgccgugg ucgacgccaa cucccaccuc cugcgccuca agggcgagac cgcgaucggg      120

gcccggcugu uccgcgaggg cgagagcgcc gccggccggc gcgccucccg gacgacccgc      180

cggcgccuga gccacaaccg guggcgccuc cgguuccugc aggacuucuu cgacccgcac      240

aucuccagca ucgaccccga cuucuuccug cgccagaagu acuccgagau cagccccaag      300

gacaaggagc gguucaagua cgagaagcgc cucuucaacg accggaccga cgcgcaguuc      360

uacaagcagu accccaccau guaccaccuc cgccugcacc ucaugaacga cgccuccaag      420

gccgacccgc gggagaucuu cuucgccauc caccacaucc ucaagagccg cgggcacuuc      480

cugacgcccg gcgacgcgaa gaacuucaac accgacgacg uggaccucaa gcacaucuuc      540

cccgcccuga ccgaggccua cgcccaggcc uaccccgacc ugacccucuc cuucgagccg      600

agcaaggcgg agaaguucaa guccauccug cuggacgagg acgccacgag caacgacacc      660

cagaaggccc ucgugaagcu gcugcucucc gacgacgggg acaaggaggu ccugaagcag      720

cagaagaagg ugcugaccga guucgccaag gccguggcgg gccucaaggu caaguucaac      780

guggcccugg ggaucgaggu gaccgacgcc gacgucgaga aguggcacuu cggcauggcc      840

cagcuggagg acaaguggag cgacaucgag cccucccuca cggaccaggg cauggagauc      900

uucgagcaga uccaggaccu guaccgggcc cgccugcuca acgggaucgu gcccgcgggc      960

aagacccuga gccaggccaa gguggacgac uacgaccagc acugcaagga ccugaagcug     1020

uucaaggagu accugaagca gcugaacgac cacgaccucg ccaagcggau ccgccugcug     1080

uacgaccgcu acaucgacgg ggacgacgcc aagcccuucc uccgggacga cuucgucaag     1140

gcccugaaga aggaggugac cgcgcacccg accgagcugg ccgagagccu ccuggagcgc     1200

auggagcagg acaacuucau gcugaagcag cggacgaagg ccaacggcuc cauccccgug     1260

cagaugcagc agcgcgagcu cgacaagauc aucgccaacc aggcggagua cuacagcugg     1320

cuggccgccg agaaccccgu caaggagcac cgggacucca agcccuacca gcuggacgag     1380

cugcucaacu uccgcauccc guacuacgug ggccccauga ucaccaagaa gcagcaggag     1440

aaguccgggc agaacguguu cgccuggaug guccggaagg accccagcgg caacaucacc     1500

cccuacaacu ucgacgagaa gguggaccgc gaggccagcg cgaacgccuu cauccagcgg     1560

augaagacca cggacaccua ccugaucggg gaggacgugc ugccgaagca gucccuccug     1620

uaccagaagu acgagguccu gaacgagcuc aacaacgugc gcgugaacgg cgagagccug     1680

uccaccgacc agaagaagcg gcugauccgc gagcuguucg agcggcacag cuccgucacc     1740

cugaagcagu ucguggagaa ccuggucgcc cacggggacg uggccaagcg ccccgacgug     1800

cggggccucg ccgacgagaa gcgcuuccug agcucccuga gcacguacca ccagcuccgg     1860

ggcauccugc acgacgcgau cgacgaccgc gggaagcagu ccgacaucga gaacaucauc     1920

accuggagca ccgucuucga ggacgccucc aucuucaaga gcaagcuggg cgagaucucc     1980

uggcucgaca gcaagacccg gacgacccug agcaacaucc gcuaccgcgg guggggccag     2040

uucucccgga agcugcucga cgggcugaag cugggcaacg gccacaccau cauccaggag     2100

cucaugcuga gcucccgcaa ccugaugcag auccucaccg acgagacgcu gaagaaggag     2160

auggccaccc ugaaccagga gcggcucaag cccgacagca ucaacgaggc caucgacgcc     2220

gcguacaccu cccccagcaa caagaaggcc auccgccagg ugcugaaggu ggucgacgac     2280

aucaagcacg ccgccaacga ccaggcgccg uccuggcugu acaucgagac cgccgacggg     2340

ggcgggcacc ccggcaagcg gacgcucagc cgccagaagc agauccaggc caucuacgag     2400

aacaccgcca aggagcugau cgacuccgcc gugcgcgggg agcugaagga caagaucgcg     2460

gacaagacca acuucagcga ccggcucgug cuguacuuca ugcagggcgg ccacgacauc     2520

uacaccggga ccgcccugaa caucgaccag cucuccagcu acgacaucga ccacauccug     2580

ccccaguccc ugaucaagga cgacagccuc gacaaccggg uccuggugca cgccaagauc     2640

aaccgcgaga agaacgccga guucgccucc acgcuguucg cggccaagau ggccagcacc     2700

uggcaccggu ggcgcgacgc cggccucauc uccgggcgga agcugaagaa ccugcucaug     2760

cgccccgacg agguggacaa guacgccaag ggcuucgucg cgcggcagcu gguggagacc     2820

cgccagauca ucaagcugac cgagcagauc guggccgccc aguacccgga cacgaagauc     2880

aucgccguca aggcgggccu cagccaccag cugcggcagg agcuggacuu ccccaagaac     2940

cgcgacguga accacuacca ccacgccuuc gacgccuucc ucgccgcccg gaucgggacc     3000

uaccugcuga agcgcuaccc caaccucgcg ccguucuuca ccuacggcga guucaccaag     3060

acgaaccuga agaagcugca gagcuucaac uucaucgggg cccucaccca caccgacggc     3120

aagaucaccu ccaaggacac gggggaggug cugugggaca accagcggga caucgaggag     3180

cuggaccaga ucuacaacuu caagcgcaug cucaucaccc acgaggucua cuucgagacc     3240

gccggccugu ucaagcagac cguguacgcc gccaaggaca gcaaggagcg cggcgggucc     3300

cggcagcuga uccccaagaa gaagggcuac agcacgggga ucuacggcgg guacacccag     3360

gagaccggcu ccuacaacgc gcucgugcgc cugaccaagg ccggcaaggu cguguaccgg     3420

guguucaaga ucagcacgca gaacgccgcc gagaucgccg cggcccgcuc ccugagcgcc     3480

gaggagggga acgagcggcu cugccagauc cuggagcagu cccugcucaa gaccaacaag     3540

gacaagaaga acaccgucga ccuguucgac guggugaucc cccgccuggg caaggaggcc     3600

cucuuccaca acagcaagua cgggcuguuc cuggucaacu ccgacaccua cuaccacaac     3660

uaccaggagc ucuggcugag ccgggagaac cagaagaucc ugaagaagcu guucuccacg     3720

aaguacgacc agagcgagau ggaccgcgac gcccugcacc uguucgagac caucgcgaag     3780

caggucgagc aguucuucaa gcucuacgac aucaaccagu uccggaccaa gaucuccagc     3840

gccuucgacg ccuucgagaa gcugcccgug gagggcgagg aggggaaggu ggagacccug     3900

aagcagcucc ugaucggccu gcacgccaac ccggugcgcu ccgaccucaa gaaccugggc     3960

aucaagacgc ccuucggguu ccugcaggcg ggcaagggga uccaccucga cgacgacacc     4020

gagaucaucc agcagagccc cgccggccug uucaagcggc gcgugcggcu ggccgaccug     4080

uga                                                                   4083


<210>  1901
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1901
augaccaagc ugggcaagcc cuacgggauc ggcguggaca ucgggagcaa cuccaucggc       60

uucgccgccg ucgacgagaa cagccaccuc auccgccuga aggggaagac cgugaucggc      120

gcgcggcugu ucgaggaggg caaggccgcc gccgaccgcc gggccucccg caccacccgg      180

cgccggcuca gccgcaaccg guggcgccug uccuuccugc gggacuucuu cgagagccac      240

aucaccccga ccgggcccaa cuucuucaug cgccagaagu acuccgagau cagccccaag      300

gacaaggcgc gguacaagua cgagaagcgc cucuucaacg accggacgga cgccgaguuc      360

uaccagcagu accccaccau guaccaccuc cgcaaccggc ugcucaccga cccguccaag      420

gccgacgugc gcgagaucua cuucgccauc caccacaucc ucaagagccg gggccacuuc      480

cugaccuccg gguacgcgaa ggacuucaac acgaacaagg ucgcccugaa cgagaucuuc      540

cccgcccugc aggacgccua cgcccaggug uaccccgacc uggacaucac cuucgacgag      600

aacaagguga acgaguucaa gaccguccuc cugaacgaga aggcgacccc cagcgacacg      660

cagcgcgccc uggugaaccu ccugcuggcc gaggacggcg acaaggacau ccucaagcag      720

cagaagcagg uccugaccga guucgccaag gccguggugg ggcugaagac caagcucaac      780

guggcgcugg gcaccgaggu cgacuccagc gaggccacgg uguggaacuu cucccugggc      840

cagcucgacg acaagugggc cgggaucgag agcgccauga ccgacgaggg caccgagauc      900

cuggaccaga uccgggaccu guaccgcgcc cgccuccuga acgggaucgu gccggcgggc      960

aagacccugu cccaggccaa ggucgacgac uacgcccagc accacgagga ccucgagcug     1020

uucaaggacu accugaagca gcucgaggac gacggcacgg ccaaggccau ccggcagcug     1080

uacgaccgcu acaucgacgg ggacgacgcg aagcccuucg ugcgggagga cuucgugaag     1140

gcccugacca aggaggucac cgcccacccc aacgccaaga gccccgagcu ccuggagcgc     1200

cuggcgcagc cggacuucau gcucaagcag cggaacaagg ccaacggcgc cauccccgug     1260

cagaugcagc agcgcgagcu ggaccagauc aucaagaacc aguccgugua cuacgacugg     1320

cuggccgccc ccaaccccgu cgagaagcac cgcaagagca ugccguacca gcucgacgag     1380

cugcugaacu uccggauccc cuacuacgug gggccccucg ugaccgcgaa ggagcagaag     1440

gccgcccagg gcggggucuu cgccuggaug gugcggaagg accccgacgg caacaucacg     1500

ccguacaacu ucgacgagaa ggucgaccgc gaggccagcg cgaacaccuu cauccagcgg     1560

augaagacca ccgacacgua ccugaucggc gaggacgugc ugcccaagca gucccugcuc     1620

uaccagcgcu acgaggugcu gaacgagcug aacaacgucc gggugaacaa cgagaagcuc     1680

agcaucgagc agaagcagca ggucauccgc gagcuguucg agcggcacaa cuccgugacc     1740

aucaagcagu ucgccgagaa ccugcgcgcc cacggggacu acgcccacau ccccgagauc     1800

cggggccucg ccgacgagaa gcgcuuccug agcucccuga gcaccuaccg gcagcucaag     1860

ucccugcugc ccgacgcgau cgacgacccg gccaagcagg ccgacaucga gaacaucauc     1920

gccuggagca ccguguucga ggacgcggcc aucuucaaga cgaagcucaa ggagaucaac     1980

uggcuggacu cccagaccau caccaagcug agcaacaucc gcuaccgcgg guggggccag     2040

uucucccaca aguuccucaa cgggcugacc cugggcaacg gccacacgau cauccaggag     2100

cuguuccuga gcaacaacaa ccugaugcag auccucaccg acgagacccu gcagaagaag     2160

augaccgagc ugaacgccga caagcucaag gccgccaaca ucaacgacgc gaucgacgac     2220

gccuacaccu cccccagcaa caagaaggcc cugcgccagg ugcugcgggu caucgaggac     2280

aucaagcggg ccgccgacgg gcaggacccc uccuggcucu acguggagac ggcggacggc     2340

gggggcaccc ccgggaagcg cacccgggcc cgccagcacc agcugcagga gaucuacgcc     2400

aacgccgccc acgagcugau cgacagcgcg gugcggggcg agcucgagga caagaucgcc     2460

gacaaggccg acuucaacga ccgccugguc cuguacuuca ugcagggcgg gcgggacauc     2520

uacaccggca cgccgcucaa caucgaccag cuguccagcu acgacaucga ccacauccug     2580

ccccaguccc ucaucaagga caacagccug gacaaccgcg ugcuggugca cgccaucauc     2640

aaccgggaga agaacgcgac cuucgccagc accaucuacg cccagaagau gaacgccacc     2700

uggcgccagu ggcacgaggc cgggcucauc uccggccgga agcugcgcaa ccugcagaug     2760

cggcccgacc agaucgacaa guacgcgagc ggcuucgucg cccgccagcu cguggagacg     2820

cggcagauca ucaagcugac cgagcagauc guggccgccc aguacccgga gaccaagauc     2880

aucgccguca aggcggggcu gucccaccag cuccgcgagg agcuggaguu ccccaagaac     2940

cgggacguga accacuacca ccacgccuuc gacgccuucc uggccgcccg caucggcacc     3000

uaccuccuga agcgguuccc caaccugcag gcguucuuca cguacgggaa guucaagaag     3060

gccgacguga agaagcuccg cggcuucaac uucauccgcg acaucaccca cgccgaggac     3120

aagaucgucg ugaaggacac cggggaggug aucugggaca agaagcggga cgucgacgag     3180

cuggaccgca ucuacaacuu caagcggaug cugaucaccc acgaggugca cuucgagaag     3240

gccgaccucu ucaagcagac gguguacggc gccaaggaca gcaaggaggc gggcgggaag     3300

aagcagcuga ucuccaagaa gaagggcuac cccgucgaca ucuacggggg cuacacccag     3360

gagaccggga gcuaccuguc cguggugcgc cucaccaaga aggccaugua cgccgucgug     3420

aaggugagca cgcgggacgc cgcgaagcug gccgucgcca aguccaucag cgagcagaag     3480

gagaacgaga cccugaacga caucaucgac gagaagcucu ccaagaucag caagaacggc     3540

aagaucaccc acgagcuguu cgagguggug cugccgcgcg ucggccagaa gacccucuuc     3600

aagaacucca aguacaaccu guuccuggug aacagcgaca cguacaugca caacuaccag     3660

gagcucugga ugccccggga guaccagaag auguggaagg acaucucccu gagcaaguac     3720

ggggacgccc agaccgagga ccagcuggac cagaucuucg gcuucaucgu gucccagguc     3780

aacagcuacu ucaaccucua cgacaucaac caguuccgca agaagcugaa cgacgccgcg     3840

gacaaguucg ccacccugcc caugcgggac accgacgaca ugcaggggaa gaucgccacg     3900

aucggccagc uccugaucgg gcugcaggcc aacgccgcgc gcagcgaccu ccggaaccug     3960

gacaucaaga cgccccuggg ccuccugcag aucccgaacg gcaucucccu ggacagcgac     4020

accuccgugg uguaccagag ccccaccggg cuccuggagc gcgaggucca ccuguccgac     4080

cucuga                                                                4086


<210>  1902
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1902
augaagaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcguc       60

gugaccgacg acuacaaggu gccggccaag aagaugaagg uccucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acuccgggga gaccgcggag      180

gccacccgca ugaagcggac cgcccgccgg cgcuacacgc ggcgccggaa ccgcauccug      240

uaccugcagg acaucuucag ccccgagcuc aaccaggugg acgaguccuu ccugcaccgg      300

cuggacgaca gcuuccucgu ggccgaggac aagcgcggcg agcggcacgu caucuucggc      360

aacaucgccg acgaggugaa guaccacaag gaguucccca ccaucuacca ccuccgcaag      420

caccuggcgg acuccagcga gaaggccgac cuccggcugg uguaccuggc ccucgcccac      480

aucaucaagu accgcgggca cuuccugauc gacgagccca ucgacauccg gaacaugaac      540

ucccagaacc uguucaagga guuccuccug gcguucgacg gcauccaggu cgacugcuac      600

cuggccagca agcacaccga caucuccggg aucaucaccg ccaagaucag caagucccgc      660

aagguggagg ccgugcucga gcaguucccg gaccagaaga agaacagcuu cuucggcaac      720

auggucuccc ugguguucgg gcugaugccc aacuucaaga gcaacuucga gcucgacgag      780

gacgccaagc ugcaguucuc ccgggacagc uacgacgagg accuggagaa ccuccugggc      840

aucaucggcg acgaguacgc ggacguguuc gucgccgcca agaaggugua cgacuccauc      900

cugcucagcg ggauccugac gaccaacaac cacagcacca aggcgccccu guccgccagc      960

augaucgacc gcuacgacga gcacaacucc gacaagaagc uccugcggga cuucauccgc     1020

accaacaucg gcaaggaggu guucaaggag gucuucuacg acacgagcaa gaacggguac     1080

gccggcuaca ucgacggcaa gaccaaccag gaggacuucu acaaguaccu gaagaaccuc     1140

cugcagaagg uggacggggg cgacuacuuc aucgagaaga ucgagcggga ggacuuccug     1200

cgcaagcagc gcaccuucga caacgggucc aucccccacc aggugcaccu cgacgagaug     1260

aaggccaucc ugcggcgcca gggcgaguuc uacccguucc ugaaggagaa cgccgagaag     1320

auccagcaga uccucaccuu caagaucccc uacuacgucg ggccccuggc ccggggcaac     1380

agccgcuucg ccugggcguc cuacaacagc aacgagaaga ugacgcccug gaacuucgac     1440

aacgugaucg acaagaccuc cagcgcccag gccuucaucg agcggaugac caacaacgac     1500

cuguaccucc cggaccagaa ggugcugccc aagcacuccc ugcucuacca gaaguucgcc     1560

gucuacaacg agcugaccaa gaucaaguac gugacggaga ccggcgaggc ccgccuguuc     1620

gacgucuucc ugaagaagga gaucuucgac gggcuguuca agaaggagcg gaaggugacc     1680

aagaagaaga uccugaacuu ccuggacaag aacuucgacg aguuccgcau caccgacauc     1740

cagggccucg acaacgagac ggggaacuuc aacgcgagcu acggcaccua ccacgaccug     1800

cugaagauca ucggggacaa ggaguucaug gacuccagcg acaacgugga cguccucgag     1860

gacaucgugc ugucccugac ccucuucgag gaccgggaga ugaucaagca gcgccugcug     1920

aaguacgagg acaucuucag caagaaggug aucgccaacc ucacccggcg ccacuacacg     1980

ggcuggggcc ggcuguccgc caagcugauc aacgggauca aggacaagca cagccgcaag     2040

accauccucg acuaccugau cgacgacggc cacuccaacc ggaacuucau gcagcugauc     2100

aacgacgaca accucagcuu caaggacgag aucgccaaca gccaggucau cggggacggc     2160

gacgaccugc accagguggu gcaggagcug gcgggguccc ccgccaucaa gaagggcauc     2220

cuccagagcc ugaagaucgu cgacgagcug gugaagguga ugggcuacaa ccccgagcag     2280

aucgucgugg agauggcccg cgagaaccag accaccgccc gggggcgcaa caacucccag     2340

cagcggcucg gcagccugac gaaggccauc caggacuucg gguccgacau ccugaagcgc     2400

uacccggugg agaacaacca gcuccagaac gaccagcugu accuguacua ccuccagaac     2460

ggcaaggaca uguacaccgg cgacacccug gacauccaca accugagcca guacgacauc     2520

gaccacauca ucccccaguc cuucaucaag gacaacagcc ucgacaaccg cguccugacc     2580

aacuccaaga gcaaccgggg gaaguccgac aacgugccca gcaacgaggu ggucaagcgc     2640

augaagggcu ucuggcugaa gcagcucgac gcgaagcuga ucucccagcg gaaguucgac     2700

aaccugacga aggccgagcg cgggggccuc agcgccgagg acaaggccgg guucaucaag     2760

cggcagcugg uggagacccg ccagaucacc aagcacgugg cccggauccu ggacgagcgc     2820

uucaaccggg acuucgacaa gaacgacaag cgcauccgga acgucaagau cgugacccuc     2880

aaguccaacc uggugagcaa cuuccgcaag gaguucggcu ucuacaaggu ccgggagauc     2940

aacaacuucc accacgcgca cgacgccuac cugaacgccg ugguggccaa ggcccuccug     3000

auccgcuacc ccaagcugga gccggaguuc gucuacggcg aguaccccaa guacaacucc     3060

uaccgggagc gcaagagcgc gaccgagaag auguucuucu acagcaacau caugaacaug     3120

uucaagacga ccaucaagcu cgccgacggg cggguggugg agaagcccgu caucgaggcc     3180

aacgaggaga ccggcgagau cgccugggac aagaccaagc acuucgcgaa cgugaagaag     3240

gugcuguccu acccccaggu cagcaucgug aagaaggugg aggagcagac cgggggcuuc     3300

uccaaggaga gcauccugcc gaagggcggg uccgacaagc ucaucgcccg caagacgaag     3360

aacaacuacc ugagcaccca gaaguacggc ggguucgacu cccccaccgu cgccuacagc     3420

aucauguucg uggccgacau cgagaagggc aaguccaagc ggcugaagac ggugaaggag     3480

augaucggga ucaccaucau ggagcgcagc cgguucgagu ccaacagcgu caccuuccuc     3540

gaggagaagg gcuaccgcaa cauccgcgag aacaccauca ucaaguuccc caaguacucc     3600

cuguucgagc uggagaacgg gcggcgccgg cuccuggcca gcgcgaucga gcugcagaag     3660

ggcaacgaga uguuccuccc ccagcaguuc gugaaccugc uguaccacgc ccagcacgcc     3720

aacaaggagg acuccgugau cuaccucgag aagcaccgcc acgagcugag cgagcuguuc     3780

caccacauca ucggcgucuc cgagaagacg auccucaagc cgaaggugga gaugacccug     3840

aacgaggccu ucgagaagca cuucgaguuc gacgagguga gcgagcuggc ccaguccuuc     3900

aucagccucc ugaaguucac cgcguucggg gcccccggcg gguucaaguu ccuggacgcc     3960

gacaucaagc agagcaaccu ccgguaccag accgucacgg aggugcuguc cagcacccug     4020

auccaccagu ccgugaccgg ccucuacgag acccgcaucg accugagcaa gcucgggggc     4080

gaguga                                                                4086


<210>  1903
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1903
augggcaacu acuaccuggg gcucgacauc ggcaccaaca gcguggggug ggccgucacc       60

gacgagaacu acaacaucga gcgcuucaag aagaagaaca uguggggcau ccggcuguuc      120

gacgaggcca agacggcgga gucccgccgg auccaccgcc aggcccggcg caugcggcag      180

cgcaagaucc agcggcugaa ccuccugaug gagcuguucg acguggagau cagcaaggug      240

gacgaguccu ucuucauccg caagaaggag agcgaccucc acuacgagga caagucccag      300

gacagccggu acgcccuguu caacgacaag gaguucaacg acaaggacua ccacaacaag      360

uaccccacca ucuuccaccu gaucauggac cucaucgaga acgacgagaa gaaggacauc      420

cgccugcugu accucgccug ccacuaccug aucaagaacc gggggcacuu caucuucgag      480

ggccagaagu ucgacgucaa cuccagccug aacaacaaca ucaacaagcu caagucccac      540

cuggaccagu ucuacaacau cugccuggac uucgacaacg acaagcucau cgagauccug      600

gugaaccaga accugaacau cagcgagaag uccaagaaga ucaaggagcu ccugcgcaag      660

accaaccugg aggugggcau cgccaaccuc aucgccgggg gcagcaucaa gcuguccaac      720

auguucgacg acgacgagcu gaagaaccuc gacuacaaca gccuguccuu caagaacagc      780

aacuucgacg aggggcggga gcuguucgag gagguccucg aggaccgcuu cgagcuggug      840

gagauccuga aggacaucca cgacgcgucc gugcucgaga ccaugcugag cuccgccagc      900

aucagcccgg acggcgggaa guacaucucc agcuccauga ucaacagcua caacaagcac      960

aagcgggacc ugaagcuccu gaagaagauc aucaagaagu acaagaaguc cgaguacgac     1020

aacaucuucc gcgacgagaa caucaagaac aacuaccuga gcuacacgcg gaccggcauc     1080

aacaagaaca agaaggucca ggccgaggac aacaccgacu ccgacggcuu cuacaaguac     1140

aucgugaaga uccucaagga caucaaggag aacgacagcg acgaggacau caaguacauc     1200

cuguccgaga ucgacaagaa gaacuucgug cccaagcagc gcaccaagga gaacagcguc     1260

auccccuacc agcugcggga gaucgagcuc cgcaagauca ucgacaacca guccaaguac     1320

cacaaguucc ugaacgaggu ggacgagaag gggaucagcg ugaaggagaa gaucaucucc     1380

cugcucacgu uccgcauccc cuacuacauc ggcccgcuga accccagcua caacgaggag     1440

aagaacuuca ccaccuccug ggucaagcgg cuggcgaagg ggaaggugac ccccuggaac     1500

uucgaggaca ugguggacau cgacgccagc cgcgaggccu ucaucgagaa caagcucaac     1560

aagugcuccu uccugcugga cgagacgguc cugcccaagg acagccuccu guaccaggag     1620

uacauggugc ugaacgagcu caacaaccug aagaucaacg gccaguccau caacaccgac     1680

cugaagaagu ccaucuacga ggagcuguuc aagaaguaca agaaggugac ccggaagcgc     1740

cugaucagcu acaucaagga gaaggagaac augagcggcg acaucgucau caccgggauc     1800

gacggcgacu ucaagcaggg gcucaagagc uacaucgccu ucaaggacau caucggcgag     1860

aagaucgacg acgacaucua ccggaaggag guggagaaga ucauccgcga caucucccuc     1920

uacggggacg acaagagcuu cauccagaag aagaucaaga acgagaacaa gcuggaccug     1980

uccgacgacc agaucagcaa gaucgccaag cucaaguuca aggacugggg ccgguucucc     2040

aagaagcugc uggugggcau caaggcgagc gacaagaaca cgggggagau cgacaacauc     2100

aucggcuuca ugcgcaacua caacuacaac aucaugcagc ucauggccaa cgucuucacc     2160

uucaaggacg agaucgagaa gcugaacucc aaguacuaca ccgacgagaa gaucaacauc     2220

gacuaccugg acgaccucua ccugagcucc ccggccaagc ggaugcugug gcagagcuuc     2280

cagaucgugg acgagaucaa gaacaucaag aacuccgacc ccaagaagau cuucaucgag     2340

auggcccgca gcaaggagga caagcccacc cggaagaugu cccgcaagag ccggcucgag     2400

aagcuguaca cggacuccaa gaagcaguuc aucaagcugu ucgggcccga cgaguacgac     2460

aagcucaaca acgagaucaa gaacaagagc gagagcgacu uccgcuggga caaccuguac     2520

cuguacuaca cccaguucgg ccggugcaug uacucccucg acccgaucga ccuggaggag     2580

cugagcaaca agaaccucua cgaccaggac cacaucuacc ccaaguccaa gaucuacgac     2640

gacagcaucg agaaccgcgu ccuggugaag aagcagcuga accucgacaa guccaacgag     2700

uaccccaucc ccgaccgggu gcugaacaag gacaagaaca agcugcucgc cuucuggaag     2760

augcugaacg agaaggagcu gaucggggac aagaaguacg cgcgccucau ccgcaagagc     2820

ccguucaccg acgaggagcu gggcgacuuc aucgcccggc agcuggucga gacccgccag     2880

gccacgaagg agaccgccaa caugcucaag cggcugugcc ccgacuccaa gaucguguac     2940

gugaaggcca agaacgucag cgacuuccgc cggaaguucg acauccugaa gugccgcucc     3000

aucaacgacc uccaccacgc gcacgacgcc uaccugaaca ucgugguggg caacgucuuc     3060

gacaccaagu ucaccagcaa cccccugaac uucaucaaga acaacuccaa gaaccggagc     3120

uacaaccucg agaagaucua cgacuacgac gugauccgca acgagaagau cgccuggcug     3180

gccgagcgga aggaggagaa ccaggacggg uccaucgcca cggugcugaa gaaccucagc     3240

aagaacgaca uccgcgucac ccggcgcacc uacaucaagu gcgaggaccu guucgacgag     3300

aacaucgugc ggaagggcaa cgggaaguuc cccaucaagg agaacaacaa caaguccgac     3360

accaacaagu acggcgggua cagcggcauc aagaacgcgu acuucgugcu gcucgaggac     3420

gagaagcaca accgcauccu ggaggccauc ccguccuuca ucuacaagaa ggagaagaac     3480

ggcgagaagg ggagcgucau caacuaccug cagaacaucc ucggcaucaa guccgccaag     3540

auccugcggg acaagaucaa gaucgggagc cugcucaaga ucgacaaguu ccccuacaac     3600

cuggccggca agagcggcaa cgggauccug aucuccggcg gggugcagcu cauccuggac     3660

aagaacacga gcaaguacau caagaagauc gagaacuaca aguacaagcu ggacgagaac     3720

aaggacuacc agaucaacau cuucgacaag cucuccaagg aggagaacau caagcuguuc     3780

gaccuguucg uggacaaguu cgagaacagc aucuacaaga accgcgucaa caucaagcuc     3840

aaggagauca agcuggcgcg gcccaaguuc gagcaccugu ccaucgagga gcagaucaag     3900

acccucaccc agaugcugau ccuguucggc cgcgagaaca acgccgugaa ccucgaguac     3960

aucgggcugc ccaagaacac cggcaagacg aagaucaaca agaagaucaa caacaaggac     4020

aucaagauca ucaaccagag caucaccggc cuguacgaga ccgaggagga ccucgccaac     4080

aucuga                                                                4086


<210>  1904
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1904
augaccaagc ugaaccagcc cuacggcauc gggcucgaca ucggcagcaa cuccaucggg       60

uucgccgugg ucgacgccaa cagccaccuc cugcgccuca agggcgagac cgcgaucggg      120

gcccggcugu uccgcgaggg ccaguccgcc gccgagcggc gcgagagccg gacgacccgc      180

cggcgccugu cccggacccg cuggcggcuc agcuuccugc gcgacuucuu cgccccgcac      240

aucaccaaga ucgaccccga cuucuuccug cggcagaagu acuccgagau cagccccaag      300

gacaaggacc gcuucaagua cgagaagcgg cuguucaacg accgcacgga cgcggaguuc      360

uacgaggacu accccuccau guaccaccuc cggcugcacc ugaugaccca cacccacaag      420

gccgacccgc gcgagaucuu ccuggccauc caccacaucc ucaagagccg gggccacuuc      480

cugacccccg gggccgcgaa ggacuucaac acggacaagg uggaccugga ggacaucuuc      540

cccgcccuga ccgaggccua cgcccaggug uaccccgacc ucgagcugac cuucgaccug      600

gccaaggcgg acgacuucaa ggccaagcuc cuggacgagc aggccacccc guccgacacg      660

cagaaggccc uggucaaccu ccugcugagc uccgacggcg agaaggagau cgugaagaag      720

cgcaagcagg uccucaccga guucgccaag gcgaucaccg ggcugaagac caaguucaac      780

cuggcccucg gcacggaggu ggacgaggcc gacgccagca acuggcaguu cuccaugggg      840

cagcuggacg acaaguggag caacaucgag accuccauga ccgaccaggg caccgagauc      900

uucgagcaga uccaggagcu guaccgggcc cgccuccuga acggcaucgu gcccgcgggg      960

augagccugu cccaggccaa gguggccgac uacggccagc acaaggagga ccucgagcug     1020

uucaagacgu accugaagaa gcucaacgac cacgagcugg ccaagaccau ccgcgggcug     1080

uacgaccggu acaucaacgg cgacgacgcc aagcccuucc uccgcgagga cuucgucaag     1140

gcgcugacca aggaggugac cgcccacccc aacgagguga gcgagcagcu gcucaaccgg     1200

augggccagg ccaacuucau gcugaagcag cgcacgaagg ccaacggggc gaucccgguc     1260

cagcugcagc agcgggagcu cgaccagauc aucgccaacc agagcaagua cuacgacugg     1320

cuggccaagc ccaaccccgu ggagaagcac cgcgccucca agcccuacca gcuggacgag     1380

cugcucaccu uccggauccc guacuacgug ggcccccuga ucacccccaa ggagcaggcc     1440

gagagccgcg agaacgucuu cgcguggaug auccggaagg accccuccgg gaacaucacc     1500

ccguacaacu ucgacgagaa gguggacugc cccgccagcg ccaacauguu cauccagcgc     1560

augaagaaca cggacaccua ccugaucggc gaggacgugc uccccaagcg gucccugcug     1620

uaccagaagu acgagguccu caacgagcug aacaacguga gcaucgacgg gcgccggcug     1680

ggcgccgacc agaagcagcg ccucauccgg gagguguucg agcgccacuc cagcgucacc     1740

aucaagcagg ucgccgagaa ccugguggcg cacggcgacu ucgccaagcg gcccgagauc     1800

cgcgggcugg ccgacgagaa gcgguuccuc uccagccugu ccaccuacca ccagcugaag     1860

aagauccucc acgaggccau cgacgacccg acgaaggagc acgacaucga gaacaucauc     1920

accuggagca ccguguucga ggacgccgcg aucuucaaga ccgagcuggc caagaucucc     1980

uggcuggaca gcgacacgau caaggcccuc uccaacaucc gcuaccgcgg cugggggcag     2040

uucagccgga agcugcugga cggccucaag cuggggaacg gccacaccgu gauccaggag     2100

cugaugcucu ccaaccacaa ccugaugcag auccugaccg acgagacccu caagaccacg     2160

auggccgagc ugaaccagga caagcugaag gcggacgaca ucgaggacgu caucaacgac     2220

gccuacacca gccccuccaa caagaaggcc cuccgccagg ugcugcgggu ggucgaggac     2280

aucaagcacg ccgccaacgg ccaggacccc agcuggcugu acaucgagac cgcggacggg     2340

cccggcaccg ccgggaagcg cacgcagagc cggcagaagc agauccagac cguguacgcc     2400

aacgccgccc aggagcucau cgacuccgcg gugcgcggcg agcuggagga caagaucgcc     2460

gacaaggcca gcuucaccga ccggcugguc cucuacuuca ugcagggcgg gcgcgacauc     2520

uacaccggcg ccccgcugaa caucgaccag cugucccacu acgacaucga ccacauccuc     2580

ccccagagcc ugaucaagga cgacucccug gacaaccggg ugcucgugaa cgccacgauc     2640

aaccgcgaga agaacaacgu cuucgcgagc acccuguucg ccuccaagau ggccgccacc     2700

uggcggaagu ggcacgaggc ggggcugauc agcggccgca agcuccggaa ccugaugcug     2760

cgccccgacg agaucgacaa guucgcccac ggguucgugg cccggcagcu cguggagacc     2820

cgccagauca ucaagcugac ggagcagauc gccgccgcgc aguacccgaa caccaagauc     2880

aucgccguca aggccggccu gucccaccag cuccgggagg agcuggacuu ccccaagaac     2940

cgcgacguga accacuacca ccacgccuuc gacgccuucc uggcggcccg gaucggcacc     3000

uaccuccuga agcgcuaccc caagcuggcc cccuucuuca ccuacgggga guucgccaag     3060

guggacguca agaaguuccg ggaguucaac uucaucggcg cccucacgca cgcgaagaag     3120

aacaucgugg ccaaggacac cggggagauc gugugggaca aggagcgcga cauccaggag     3180

cuggaccgca ucuacaacuu caagcggaug cugaucaccc acgaggucua cuucgagacc     3240

gccgaccucu ucaagcagac gaucuacgcc gccaaggaca gcaaggagcg cggcgggucc     3300

aagcagcuga ucccgaagaa gcagggcuac cccacccagg uguacggcgg guacacccgg     3360

gagaacaccg cguaccuggc cguggucaag acgguggaga agaaggccga cguguaccgc     3420

gucgugcgga ucgccaccag ccagguggcg gcccucaagg aggcccgcgc ccaguccacc     3480

gucaaggagc gggagcugcu gaagaaguuc cucaccacga aguucaccaa gaucggcaag     3540

aacgggaaga aggccaucac ccccuucgac aucgugaucc cccgcgugcc gcgggagcag     3600

cuguuccaca acaagcgcua cggcuucuuc auggucaaca gcgacacccu gaugcacaac     3660

uaccaggagc ucuggauguc ccggagcgac cagcagaucc uguccaagcu ggugaagucc     3720

gcgaagacgg uggagaacgg gcaggucggc cgccucuucg agaacaucgu ggagcagauc     3780

aacaaguacu ucccccugua cgacaucaac caguuccgga agaagcugga ccagagcaag     3840

gacaucuuca gcaagcuccc ccuggagggc aacgggacca ccagcggcaa gcgcgagacc     3900

auccagaaca uccugacggg ggccggcacc ggggccaaga acggcgaccu caagaaccug     3960

ggcaucaaga cccccuucgg guuccuccag gugccguccg gcaucguccu ggacgacgac     4020

accacccuga ucuaccagag ccccacgggg augcugaacc ggaucgugcg ccucuccgag     4080

cuguga                                                                4086


<210>  1905
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1905
augaagaacg aggagaacau cuaccugggc cucgacaucg ggaccgacag cgugggcuac       60

gccgucaccg acgacaagua caagaucaug aaguaccacg ggaacgccgc guggggcucc      120

acgaucuucg acgccggggc ccugggcgcc gaccgccggg uguuccgcag cgcccggcgc      180

cggcuggacc gccggcagga gcgcauccag cuccugcagg agaucuucgc gaacgagauc      240

gccaaggugg acgagcgguu cuucauccgc cugcucgagu ccggccugug gcgggccgac      300

gccgaggacc gcuacgucuu cuucaacgac aaggaguaca ccgacgugca guacaugcgg      360

gacuacccca ccauccacca ccugaucugc gagcucaucu acaacccgaa caagcacgac      420

gugcgccugg ucuaccuggc gugcgccugg cucgugaccc accgggggca cuuccugagc      480

aacaucaaca cggagaacau cgccgaccug guggacaucc acaacgucua caacggcuuc      540

augcgcuucu ucgaggagaa cgagaucauc ugcgacuggc gggaggugaa cguggaggag      600

cucgggcgcg uccuggccgc caacaucggc aucaacgaga aguaccggaa ccuguccacc      660

guccucuucg cgacccgcaa gcccgacaag aaggccaagg agggguuccc cuacagcgag      720

gacgccaucg ugcggcugcu ggccggcggg accugcaagc ucaaggacgu guacaucaag      780

gaggaguacg acgaguucgg guccaucacg cugggcaugg acgacgagaa gaugaccgag      840

aucauggccg acaucgggga cgacuucgac cugaucaagg cgcuccgcag ccuguacgac      900

uggggcaucc uggccaacau ccucgugaac gagaacaaca gcccccugau cagccagucc      960

aaggugaagg ucuacgagca gcaccgcgag gaccuggcca accucaagua ccuggugcac     1020

aaguacaucc cgcacaagua caacgagauc uuccgggaga uccgcggcga caacuacgug     1080

gccuacagcu accacuacga gcugaagaac gacgagaaga uggggaagaa ggccgacguc     1140

gagaaguucu ccaaguuccu ccugaagauc cuggagggca ucagcgugga cgagcaggac     1200

aagguggucu acgacaacau gaucgagcgg cucacccugc gccuguuccu ccccaagcag     1260

aagaacaccg acaaccgggu gaucccccac cagcuguacg aguacgagcu gaaguccauc     1320

cucaagaaca cgaagaacua ccugcccuuc cugaaggaga aggacagcga cgggaucucc     1380

aacgaggaca agaucaucag caucuucaag uucaagaucc cguacuucgu cggcccccuc     1440

aacugcaacu cccaguucgc guggauccag cgcaaggccg acgggaagau cuuccccugg     1500

aacuucgagg acaaggugga cuucgacgcc agcgagcagg ccuucaucca gcggaugacc     1560

aaccagugca ccuaccugcc cgaggagagc gugcugccga agcagucccu cuacuaccag     1620

aaguucaugg uccugaacga gaucaacaac aucaagaucg acggccgcaa gaucagcgug     1680

gccaugaagc aggagcugua caacgagauc uucgagaaga agaagcgggu gcgcaagaag     1740

gacgucaucg acuucuucgu guccaacggc uaccuggagg gcggcaagga ggcgcucauc     1800

agcgggcugg acgagcagau ccacaccaac cuguccagcu uccacgccuu ccggcgccuc     1860

cugcagaacc acauccuguc cguggaggac gucgagagca ucaucgagcg ggccuccuac     1920

agcgacgaca agcgccgggu gcuccuguuc cugaagaaca aguacuccuu ccucacggac     1980

gaggacauca aguacaucug caagaucaag aucaaggacu ucggccgccu gagcaagaag     2040

uuccuggugg acuucgaggg gaccugcaag gagaccggcg agaucuccac cguccuccgg     2100

cugauguggg agacgaacaa caaccugaug gagcuccuga gcgacaagua caccuucgcc     2160

aacggcauca aggagaaccg caagaaguac uacaccgagc agcagaagac ccuggacgac     2220

cggcucgacg agauguacau cuccaacagc gugaagcgca cgaucuaccg cacccugucc     2280

guggucaagg acgugaccaa ggcguucggg gugcccaaga agaucuucgu cgagauggcc     2340

cggaccaaca accccgaccu gaagggcaag cgcacgcgga cccgccagca gcaggucauc     2400

gagcucuacg ccaagugcga cgaggaggac gugcgggagc ugaagcacca gauggagagc     2460

cugggggagu acguggacaa caagcuccag uccgacaagc uguuccugua cuucauccag     2520

uucggcaaga gcgccuacag cgggaagaug aucgaccucg agaagcugau guccggcagc     2580

aagguguacg acaucgagca caucuacccc caggccuacg ugaaggacga cuccaucauc     2640

aacaacaagg uccugguguu cagcgaggag aacggcgcga agcaggacau cuacccgauc     2700

aagaccgaga uccgcaagca gauguccggg aucuggacca cguggcacca cgugggcgcc     2760

aucagcgacg agaaguacaa gcggcucacc cgcuccaccc ccuucaacga ggacgagaag     2820

uacggguuca ucaaccggca gcugaccgag acgagccagu ccaccaaggc caucgcccag     2880

auccugaacg agaaguaccc cgagaccgag aucgucuacg ugaaggccgg ccucgugagc     2940

gacuuccgcc acgaguucgg ccugugcaag ugccgguccu acaacgaccu gcaccacgcg     3000

aucgacgccu accucaacgu cguggugggg aacgucuaca acaugaaguu caaccgcaag     3060

ugguucagcg uggacuccaa guacagcauc aagaccaaga ccaucuucuc ccacagccug     3120

gugugcaacg gcaagaucau cugggacaag gagaagaugc ugcccauggu ccucaagaac     3180

gccaagaaga acaacgccca cuucacgaag uaccaguuca ugaagaccgg gggcuucuuc     3240

gaccagaugc cgugcaagaa ggccuccggg cuggugcccc ggaagaaggg ccuggacacc     3300

gagcgcuacg gcggguacaa caagagcggc aucauguucc ucacccccau gcgguacaag     3360

aucgggaaga aguccgacau caucauccug uccaucgagc ugcucuacgg caagcuguuc     3420

cugaaggacg agguguucgc gaagaaguac gccgaggagc gccuccagaa gaucaucggg     3480

aagaaggccg acgaggucag cuuccccaug ggcaugaagc cguggaagau caacacggug     3540

cugucccugg acggcuucaa cgugugcauc accgggaucg gcgggggcgg gaagugccuc     3600

agcgcccagc ccaucaugca gcugucccug cccuacgagc ucgcgaagua ccugaagaag     3660

aucgagaagu ucgucgagaa gaacaagaac aacgccaacu acaucuacga cgagcaguac     3720

gacagcgugu ccguggacga gaacaucaag cuguacgacg ucuaccucga caagcuggag     3780

aacagcaucu acaacaagcg gaucaacucc cccgaggcca uccugaagaa cggccgcgag     3840

aaguucugca agcucaacgu gcuggagcag gccgugaccc ugaucaacau ccagagcauc     3900

uucggccgga ugaccggggg cugcgaccuc gagaacaucg ggggcaagaa gcacuccgcc     3960

gcgacgguca acuucagcuc caacgugagc aacuggaaga aguacuacaa cgacgugcgc     4020

gucguguacc aguccccgag cgggaucugg gagcggcgcu cccagaacau ccuggagcug     4080

cucuga                                                                4086


<210>  1906
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1906
augaacgagc ccuacggcgu ggggcuggac aucggcacca acagcgucgg guggaccgug       60

guggacauga acggccgcgu ccggaaggug aaggggaaga cggcccucgg cgcccgccug      120

uucaaggagg gcgcgaccgc cgaggaccgg cgcggguucc ggaccacccg ccggcgccug      180

aagcggguga aguggcgccu ccggcugcug cgcgaguucu ucgaccagcc gaucuccaag      240

aucgacccca acuucuucgc ccggcgcaag uacagcgaca ucagcccccg ggaccccaac      300

uacaacggcc ucgccaagac gcuguucaac gaccgcaccg acaaggaguu cuacgacgac      360

uacgccacca ucuaccaccu ccgggacaag cugaugaccu ccaaccgcaa guucgacauc      420

cgggagaucu accuggcgau ccaccacauc gucaaguacc gcgggaacuu ccugcggacg      480

ggcccggccu cccaguacgg gagcgccucc cuccaccugg ccaccagcuu ccagaagcug      540

aacgaccucu ucgcgcaguc cgaggagacc cugaaccuga agcucgugac cgacgaggcc      600

cugcugcagc agauccagca gauccucgug cgcacggacc ugagccgcuc cgagcagcag      660

cggcagaucu ggccccugau ggccguccuc accggcgcca gcgccgcgga gaagaagcgc      720

cagaagaacg ugguggucga gcuguccaag gcccuggugg gccucaaggc caagaugaac      780

guggucaccc ugaccgaggu ggacgccgcc guggucaagg acuggacguu caccauggag      840

gagagccagg acaagcugcc cgagaucgag gagcagcucu ccgagguggg gcagcagauc      900

auggacgagg ugauccagcu guacgcgagc gucaaccugg cccagcucau ccccgccggc      960

aagcgguucu cccagcacau gguggagaag uacaagcacc acgagaagaa ccuggagcug     1020

cucaaggccu acauccacag ccaguccgac agcaagcgcg ggcgggagau ccgcgccacc     1080

uacgaccggu acaucgacgg cguggacagc aagccgguca cccaggagau guucuacaag     1140

gaccugauga aguacgugga ggcggacgcc acguccaacc accuggccgc cgagaucaag     1200

gacgagaucg acagcgagca guucaugccc aagcuccgca ccaagcagaa cggcuccauc     1260

cccuaccagg uccagcagua cgagcuggac cagaucauca gccaccagaa gaaguacuac     1320

cccuggcugg gggaggagaa cccgguggcc gagcggcgcg gcaaguuccc cuacaagcuc     1380

gacgagcugg ugggguuccg gguccccuac uacgugggcc cccugaucac caaggaggac     1440

cagcaggcga ccuccggggc cggcuucgcc uggauggugc gcaaggccga cggcccgauc     1500

acgcccugga acuucgacca gaaggucgac cggaucgcga gcgccaccgc cuucauccag     1560

cgcaugcaga ccaccgacac guaccucauc ggggaggacg ugcugcccgc ccggucccug     1620

aucuaccagc gcuucauggu gcucaacgag cugaacaaca ugcgggucga ggaccgcaag     1680

cuggcgcccc agcagaagca gcggcuguac aaccaggugu ucaagcagca ccagcacgug     1740

agcgucaaga acauccagca gaaccucaug gacgccggcg aguaccgcaa gaccccgcag     1800

aucaccgggc uggccgaccc caagggcuuc aacuccagcc uguccaccua ccacgacuuc     1860

aagaagaucc uccuggaggc caucgccgac gagcacaagc gcgccgacau cgagaagauc     1920

auccugugga gcacgaccuu cgaggacucc gcgaucuuca agcagaagcu cgaggaggug     1980

gccuggcuga ccgacgccca gcggaagcag cugagcgggc uccgcuaccg gggcuggggc     2040

cagcuguccc acaagcugcu caccgccuuc aaggacgaca aggggcgcag caucauggac     2100

ggccuguggg agacguccga caacuucaug cagcugcgga agcagcccgc caucgagacc     2160

cagaucaagg aggcgaacca ggccaaccuc accgacgccg acauccagga caccaucaac     2220

gagcuguaca ccagccccca gaacaagaag gccauccgcg aggugaugcu gguccucgac     2280

gacaucaaga acgcgaugca cgggcagacg ccguccugga ucuucgugga ggccgcccgg     2340

ggcgggggcg uggccggccg ccggacccag agccgcagcu cccagaucgu cgaggccuac     2400

aaggggaccg cgaaggagau cgugagcgag aaggugcagc acgagcugaa cgagaagauc     2460

aaggccaagg ccgacuucaa cacccggcug guccucuacu uccugcagaa cggccgcgac     2520

cuguacacga acgaggccau caacaucgac cggcucuccg aguacgacau cgaccacauc     2580

cugccccaga gccuggugaa ggacgacucc cucgacaacc gcgugcugac cagcgcccgg     2640

aucaaccgcg agaagaacga caccuucgcg uccgagaagu ucgggcggaa gaugggcgcc     2700

caguggcgcg agcugcaccg gaacggccuc augacccagc gcaagcugaa gcaccuccug     2760

augcggcccg acgagaucag caagcacgcc acggacuuca ucaaccgcca gcuggucgag     2820

acccgccagg ugaucaagcu gguggaggag cucaucucca gcgaguaccc cgccgccucc     2880

aucgucgcgg ugaaggccaa ccugacccac caguuccggc agaccuucaa cuucccgaag     2940

cugcgcgagg ugaacgacua ccaccacgcc uucgacgcca gccucacggc guucaucggg     3000

auguaccugc ugaagcagua ccccaagcuc gagcgguucu ucgucuacgg caaguucgcc     3060

aagcagccca ucaaccugac ccgcuucaac aucauccgga agcuggccgu ggccgagaag     3120

ccgaucgcca acaucgagac cggggagauc cucugggaca agaccgcgga caucaaguac     3180

uucgagaagc uguacaacua caagcgccug cucgugacgc acgagguccg ggagaacuac     3240

ggcgccaugu ucaagcagac ccuguucaag gccuccuaca acaagagcaa gacccuggug     3300

cccaagaaga accacaugga gaccuccgug uacgggggcu acagcaacca ggagacggcc     3360

uaccucgcca ucguccgcau ccccuucaag uccggcuuca aguucaucgu gguggggauc     3420

cccacccgga uggucgcgaa gaucaagcac uaccagagcc ugggcgccac ccugaaccag     3480

gccacccaca aggugaucga gccgaaguuc acgaagauca gccgcaagac caagcagacc     3540

gugaucuccg acuacgaggu cgugcucccc aagguguacc uggaccaggu cgugcgggac     3600

caggugaagg ggcagaugua ccgcuucagc cugggcuccg acaaggagua ccacaacguc     3660

caggagcucu accugccccu gagcauccag caggccuucg uggggcacua cgacgagucc     3720

gacgaccagc ggagcaacga ccucgugaag gucuacgacg ccgcgcugaa gcagcugcag     3780

cgcuacuucc cccuccaccu gucccggaac uucgaccagg uggccagcca ggcccaccag     3840

uccuucgagg gccugaagaa caacguccag accagcgaca agcagcucgg caagaaggag     3900

gugcugaacu cccuguucgu ggggcuccac gccaacgcca cgcgcagcaa ccuguccguc     3960

cugggcauga gcaaggacuu cgggcggauc aaguccaacg gcaucacccu caccgaccag     4020

gcggagauca ucuaccagag cccgaccggg cuguucgagc gcaagguggc ccugaaggac     4080

cucuga                                                                4086


<210>  1907
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1907
augggcaacu acuaccuggg gcucgacauc ggcaccaaca gcguggggug ggccgucacc       60

gacgagaacu acaacaucga gcgcuucaag aagaagaaca uguggggcau ccggcuguuc      120

gacgaggcca agacggcgga gucccgccgg auccaccgcc aggcccggcg caugcggcag      180

cgcaagaucc agcggcugaa ccuccugaug gagcuguucg acguggagau cagcaaggug      240

gacgaguccu ucuucauccg caagaaggag agcgaccucc acuacgagga caagucccag      300

gacagccggu acgcccuguu caacgacaag gaguucaacg acaaggacua ccacaacaag      360

uaccccacca ucuuccaccu gaucauggac cucaucgaga acgacgagaa gaaggacauc      420

cgccugcugu accucgccug ccacuaccug aucaagaacc gggggcacuu caucuucgag      480

ggccagaagu ucgacgucaa cuccagccug aacaacaaca ucaacaagcu caagucccac      540

cuggaccagu ucuacaacau cugccuggac uucgacaacg acaagcucau cgagauccug      600

gugaaccaga accugaacau cagcgagaag uccaagaaga ucaaggagcu ccugcgcaag      660

accaaccugg aggugggcau cgccaaccuc aucgccgggg gcagcaucaa gcuguccaac      720

auguucgacg acgacgagcu gaagaacuuc gacuacaaca gccucuccuu caagaacagc      780

aacuucgacg aggggcggga gcuguucgag gagguccugg aggaccgcuu cgagcucgug      840

gagauccuga aggacaucca cgacgcgucc gugcuggaga ccaugcucag cuccgccagc      900

aucagcccgg acggcgggaa guacaucucc agcuccauga ucaacagcua caacaagcac      960

aagcgggacc ugaagcugcu caagaagauc aucaagaagu acaagaaguc cgaguacgac     1020

aacaucuucc gcgacgagaa caucaagaac aacuaccuga gcuacacgcg gaccggcauc     1080

aacaagaaca agaaggucca ggccaaggac aacaccgacu ccgacggcuu cuacaaguac     1140

aucgugaaga uccugaagga caucaaggag aacgacagcg acgaggacau caaguacauc     1200

cucuccgaga ucgacaagaa gaacuucgug cccaagcagc gcaccaagga gaacagcguc     1260

auccccuacc agcugcggga gaucgagcug cgcaagauca ucgacaacca guccaaguac     1320

cacaaguucc ucaacgaggu ggacgagaag gggaucagcg ugaaggagaa gaucaucgcg     1380

cugcugacgu uccgcauccc cuacuacauc ggcccgcuca accccuccua caacgaggag     1440

aagaacuuca ccaccagcug ggucaagcgg cuggccaagg ggaaggugac ccccuggaac     1500

uucgaggaca ugguggacau cgacgccucc cgcgaggccu ucaucgagaa caagcugaac     1560

aagugcagcu uccuccugga cgagacgguc cugcccaagg acucccugcu cuaccaggag     1620

uacauggugc ugaacgagcu gaacaaccuc aagaucaacg gccagagcau caacaccgac     1680

cugaagaagu ccaucuacga ggagcuguuc aagaaguaca agaaggugac ccggaagcgc     1740

cucaucagcu acaucaagga gaaggagaac accagcggcg acaucgucau cacggggauc     1800

gacggcgacu ucaagcaggg gcugaagucc uacaucgccu ucaaggagau caucggcgag     1860

aagaucgaca acgacaucua ccggaaggag guggagaaga ucauccgcga caucagccug     1920

uacggggacg acaaguccuu cauccagaag aagaucaaga acgagaacaa gcucgaccug     1980

agcgacaacc agaucuccaa gaucgcgaag cugaaguuca aggacugggg ccgguucagc     2040

aagaagcucc uggugggcau caaggccucc gacaagaaca ccggggagau cgacaacauc     2100

aucggcuuca ugcgcaacua caacuacaac aucaugcagc ugauggccaa cgucuucacc     2160

uucaaggacg agaucgagaa gcucaacagc aaguacuaca ccuacgagaa gaucaacgug     2220

gacuaccugg acgaccugua ccucuccgcc ccggccaagc ggaugcugug gcagagcuuc     2280

cagaucgucg acgagaucaa gaacaucaag aacuccgcgc ccaagaagau cuucaucgag     2340

auggcccgca gcaaggagga caagcccacg cggaagaugu cccgcaagag ccggcuggag     2400

aagcucuaca ccgacuccaa gaagcaguuc aucaagcugu ucgggcccga cgaguacgac     2460

aagcugaaca acgagaucaa gaacaagagc gaguccgacu uccgcuggga caaccucuac     2520

cuguacuaca cccaguucgg ccggugcaug uacagccugg acccgaucga ccucgaggag     2580

cugagcaaca agaaccugua cgaccaggac cacaucuacc ccaaguccaa gaucuacgac     2640

gacagcaucg agaaccgcgu gcucgugaag aagcagcuga accuggacaa guccaacgag     2700

uaccccaucc ccgaccgggu ccucaacaag gacaagaaca agcugcuggc cuucuggaag     2760

augcucaacg agaaggagcu gaucggggac aagaaguacg cccgccugau ccgcaagagc     2820

ccguucaccg acgaggagcu cggcgacuuc aucgcccggc agcuggugga gacgcgccag     2880

gcgaccaagg agaccgccaa caugcugcgg cgccucugcc ccgacuccaa gaucguguac     2940

gucaaggcca agaacgugag cgacuuccgg cgcaaguucg acauccugaa gugccggucc     3000

aucaacgacc ugcaccacgc ccacgacgcc uaccucaaca ucguggucgg caacguguuc     3060

gacaccaagu ucacgagcaa cccccugaac uucaucaaga acaacuccaa gaaccgcagc     3120

uacaaccugg agaagaucua cgacuacgac gugauccgga acgagaagau cgcguggcuc     3180

gccgagcgca aggaggagaa ccaggacggg uccaucgcca ccguccugaa gaaccugagc     3240

aagaacgaca uccgggugac ccgccggacc uacaucaagu gcgaggaccu cuucgacgag     3300

aacaucgugc gcaagggcaa cgggaaguuc ccccugaagg agaacaacaa caaguccgac     3360

acgaacaagu acggcgggua cagcggcauc aagaacgccu acuucguccu gcucgaggac     3420

gagaagcaca accggaucau cgaggcgauc ccguccuuca ucuacaagaa ggagaagaac     3480

ggcgagaagg ggagcgugau caacuaccug cagaacaucc ugggcaucaa guccgccaag     3540

auccuccgcg acaagaucaa gaucgggagc cugcugaaga ucgacaaguu ccccuacaac     3600

cucgccggca aguccggcaa cgggauccug aucagcggcg gggugcagcu gauccucgac     3660

aagaacacca gcaaguacau caagaagauc gagaacuaca aguacaagcu ggacgagaac     3720

aaggacuacc agaucaacau cuucgacaag cuguccaagg aggagaacau caagcuguuc     3780

gaccucuucg ucgacaaguu cgagaacagc aucuacaaga accgggugaa caucaagcug     3840

aaggagauca agcucgcccg cuccaaguuc gagcaccuga gcaucgagga gcagaucaag     3900

acccugaccc agaugcucau ccuguucggc cgggagaaca acgccgugaa ccuggaguac     3960

aucgggcucc ccaagaacac gggcaagacc aagaucaaca agaagaucaa caacaaggac     4020

aucaagauca ucaaccaguc caucaccggc cuguacgaga ccgaggagga ccuggcgaac     4080

aucuga                                                                4086


<210>  1908
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1908
augaagaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgagg acuacaaggu gccggccaag aagaugaagg ugcucgggaa cacgagccgc      120

gacuucauca agaagaaccu gcugggcgcg cuccuguucg acuccgggga gaccgccgag      180

gcccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgccuccgg      240

uaccugcagg agaucuuccu ggacgagaug aacaagcucg acagcggcuu cuuccaccgc      300

cuggacgacu ccuuccuggu ccccgaggag aagcggggca gccgcuaccc caucuucggg      360

acgcuccagg aggaggugaa cuaccacgag aacuucccca ccaucuacca ccuccggaag      420

aagcuggccg acuccaagga gaaggcggac cuccgccuga ucuaccuggc ccucgcccac      480

augaucaagu accggggcca cuuccuguac gaggagagcu ucgacuccaa caacaacagc      540

auccaggaga ucuucaacga guucaccgcc gaguaccaca acaccaucga gggguccugc      600

cugagcggcc agaucgagca ggucgaggcg auccucacgg acaagaucuc caagagcgcc      660

aagcgcgagc ggauccugaa gcuguucccg gccgagaagu ccaccgggcu guucagcgag      720

uuccugaagc ugaucguggg caaccaggcc gacuucaaga agcacuucga ccucgaggag      780

aagaccccgc ugcaguucuc caaggacacc uacgaggagg accuggagaa ccuccuggag      840

cagaucggcg acgaguucgc cgaccuguuc cuccuggcga agaagcugua cgacgccacg      900

cuccuggccg ggauccugac cgccaccgac gugagcacca aggcgccccu cuccgccagc      960

augaucaacc gcuacgagac gcaccagcag gaccuggccg cccugaagca guucaucaag     1020

aacaagcucc ccgagaagua cagcgaggug uucuccgacc ugagcaagga cggcuacgcc     1080

ggguacauca acggcaagac cacccaggag gccuucuaca aguacaucaa gaaccugcuc     1140

aucaaguucg agggcgcggu cgacuuccug gagaagaucg agcgggagga cuuccugcgc     1200

aagcagcgca ccuucgacaa cggguccauc ccgcaccagc uccaccucca ggagaugaac     1260

gccauccugc ggcgccaggg cgaccacuac cccuuccuga aggagaaccg ggagaagauc     1320

gagaagaucc ucacguuccg cauccccuac uacguggggc cccuggcccg gggcaaccgc     1380

gacuucgccu ggcugacccg gaacagcgac caggccaucc gcccguggaa cuucgaggag     1440

guggucgaca agaccuccag cgcggaggac uucaucaaca agaugaccaa cuacgaccuc     1500

uaccugcccg aggagaaggu gcugcccaag cacuccaucc ucuacgagac guucgccgug     1560

uacaacgagc ugaccaaggu caaguucauc gccgaggggc ugcgggacua ccaguuccuc     1620

gacagcggcc agaagaagca gaucaucaac cugcuguuca aggacaagcg caaggugacc     1680

gagaaggaca ucauccacua ccuccacacc guggacggcu acgacgggau cgagcugaag     1740

ggcaucgaca agcaguucaa cgccucccug agcacguacc acgaccuccu gaagaucauc     1800

aaggacaagg acuucaugga cgacgcggac aacgaggcca uccuggagaa caucguccac     1860

acccucacca ucuucgagga ccaggagaug aucaagcagu cccuggccca guacgacgcc     1920

aucuucgacc ggaaggugau gaaggcccug acccgccggc acuacacggg guggggcgag     1980

cucagcgcga agcugaucaa cgggauccgc gacaagcaga ccggcaagau gauccuggag     2040

uaccucaagg acgacggcga gaucaaccgg aacuucaugc agcugaucaa cgacgacucc     2100

cugagcuuca aggagaccau ccagaaggcc caggugaucg gggagaccga gaacaucaag     2160

cagaucgucc aggagcuccc cggcuccccg gccaucaaga aggggauccu gcagagcauc     2220

aagaucgugg acgagcuggu ggagaucaug ggcuacaacc ccgaguccau cgucguggag     2280

auggcccgcg agaaccaguu cacgaaccag gggcggcgca acagccagca gcggcucaag     2340

aagcugaccg acuccaucaa caagcugggc agcagcaucc ucgaggagca ccccgucgac     2400

aacucccagc ugcagaacga ccgccuguuc cucuacuacc ugcagaacgg caaggacaug     2460

uacaccgggg aggcccugga gcucagcaag cuguccagcu acgacgugga ccacaucauc     2520

ccccaggcgu ucaucaagga cgacucccug gacaaccgcg ugcucgucag cuacgccaag     2580

aaccggggca aguccgacga cgugccgagc cuggagaucg ugcagaagcg caaggccuuc     2640

uggcggcagc ugcucgacuc ccagcugauc agcgagcgca aguucaacaa ccugaccaag     2700

gccgagcgcg ggggccucac cgaggacgac aaggccggcu ucaucaagcg gcagcugguc     2760

gagacgcggc agaucaccaa gcacguggcg cagauccugg acgcccgcuu caacaccgag     2820

gugaccgaga aggggaagaa gaagcgggcc gucaagauca ucacgcucaa guccaaccug     2880

gugagcaacu uccgcaagga cuucgagcug uacaaggugc gggagaucaa cgacuaccac     2940

cacgcccacg acgcguaccu caacgccguc gugggcaccg cccugcugaa gaaguacccc     3000

aagcucgagc ccgaguucgu guacggggac uaccccaagu acaacuccua ccgcgagcgg     3060

aagaccgcca ccgagaaggu cuacuucuac agcaacauca ugaacuucuu caagacgacc     3120

gugaagcugg ccgacggcuc cgagaagaag cgccaccaga ucgagaccaa cgaggagacc     3180

ggggagaucg ugugggacaa gacgaagcac uucgagaucg uccggaaggu gcugagcuac     3240

ccccagguga acaucgucaa gaagaccgag gagcagaccg ugggccagaa cggcgggcug     3300

uucgacaaca acaucguguc caagaacaag gucguggacg cgagcaagcu gauccccauc     3360

aagagcggcc ugucccccga gaaguacggg ggcuacgccc gccccaccau cgccuacagc     3420

gugcucguca ucgccgacau cgagaagggg aaggccaaga agcugaagcg gaucaaggag     3480

auggugggca ucacgaucca ggacaagaag aaguucgagg cgaacccgau cgccuaccug     3540

gaggagugcg gcuucaagaa caucaacucc aaccacauca ucaagcuccc caaguacagc     3600

cuguucgagu ucgacgacgg gcagcgccgg cugcucgccu ccagcaucga gcugcagaag     3660

ggcaacgagc ugauccuccc cuaccaccug accgcccugc ucuaccacgc ccagcgcauc     3720

aacaagaucu ccgagccgau ccacaagcag uacguggagg cgcaccagaa cgaguucgag     3780

gagcugcuga ccaccaucau cagccucucc aagaaguaca uccagaagcc caacgucgag     3840

agccugcugc agcaggccuu cgaccagacg gacgaggaca ucuaccagcu cuccgagagc     3900

uucaucuccc ugcugaagcu caucagcuuc ggggcccccg gcgccuucaa guuccugggg     3960

guggacaucu cccagagcaa cgugcgguac caguccguca gcuccugcuu caacgcgacc     4020

cugauccacc agagcaucac cggccucuac gagacccgca ucgaccugag caagcugggc     4080

gaggacuga                                                             4089


<210>  1909
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1909
augaacgagc ccuacggcgu ggggcuggac aucggcacca acagcgucgg guggaccgug       60

guggacauga acggccgcgu ccggaaggug aaggggaaga cggcccucgg cgcccgccug      120

uucaaggagg gcgcgaccgc cgaggaccgg cgcggguucc ggaccacccg ccggcgccug      180

aagcggguga aguggcgccu ccggcugcug cgcgaguucu ucgaccagcc gaucuccaag      240

aucgacccca acuucuucgc ccggcgcaag uacagcgaca ucagcccccg ggaccccaac      300

uacaacggcc ucgccaagac gcuguucaac gaccgcaccg acaaggaguu cuacgacgac      360

uacgccacca ucuaccaccu ccgggacaag cucaugaccu ccaaccgcaa guucgacauc      420

cgggagaucu accuggcgau ccaccacauc gucaaguacc gcgggaacuu ccugcggacg      480

ggcccggccu cccaguacgg gagcgccucc cuccaccugg ccaccagcuu ccagaagcug      540

aacgaccucu ucgcgcaguc cgaggagacc cugaaccuga agcucgugac cgacgaggcc      600

cugcugcagc agauccagca gauccucgug cgcacggacc ugagccgcuc cgagcagcag      660

cggcagaucu ggccccugau ggccguccuc accggcgcca gcgccgcgga gaagaagcgc      720

cagaagaacg ugguggucga gcuguccaag gcccuggugg gccucaaggc caagaugaac      780

guggucaccc ugaccgaggu ggacgccgcc guggucaagg acuggacguu caccauggag      840

gagagccagg acaagcugcc cgagaucgag gagcagcucu ccgagguggg gcagcagauc      900

auggacgagg ugauccagcu guacgcgagc gucaaccugg cccagcucau ccccgccggc      960

aagcgguucu cccagcacau gguggagaag uacaagcacc acgagaagaa ccuggagcug     1020

cucaagaccu acauccacag ccaguccgac agcaagcgcg ggcgggagau ccgcgccacc     1080

uacgaccggu acaucgacgg cguggacagc aagccgguca cgcaggagau guucuacaag     1140

gaccugauga aguacgugga ggccgacgcg accuccaacc accuggccgc cgagaucaag     1200

gacgagaucg acagcgagca guucaugccc aagcuccgca ccaagcagaa cggcuccauc     1260

cccuaccagg uccagcagua cgagcuggac cagaucauca gccaccagaa gaaguacuac     1320

cccuggcugg gggaggagaa cccgguggcc gagcggcgcg gcaaguuccc cuacaagcuc     1380

gacgagcugg ugggguuccg gguccccuac uacgugggcc cccugaucac caaggaggac     1440

cagcaggcca cguccggggc gggcuucgcc uggauggugc gcaaggccga cggcccgauc     1500

acccccugga acuucgacca gaaggucgac cggaucgcca gcgcgaccgc cuucauccag     1560

cgcaugcaga ccacggacac cuaccucauc ggggaggacg ugcugcccgc ccggucccug     1620

aucuaccagc gcuucauggu gcucaacgag cugaacaaca ugcgggucga ggaccgcaag     1680

cuggcgcccc agcagaagca gcggcuguac aaccaggugu ucaagcagca ccagcacgug     1740

agcgucaaga acauccagca gaaccucaug gacgccggcg aguaccgcga ggccccgaag     1800

aucaccgggc uggccgaccc caagggcuuc aacuccagcc uguccaccua ccacgacuuc     1860

aagaagaucc uccccgaggc caucgccgac gagcacaagc gcgccgacau cgagaagauc     1920

auccugugga gcacgaccuu cgaggacucc gcgaucuuca agcagaagcu ggaggaggug     1980

gccuggcuca ccgacgccca gcggaagcag cugagcgggc ugcgcuaccg gggcuggggc     2040

cagcucuccc acaagcugcu gaucgccuuc aaggacgaca acgggcgcag caucauggac     2100

ggccucuggg agaccuccga caacuucaug cagcugcgga agcagcccgc caucgagacg     2160

cagaucaagg aggcgaacca ggccaaccug accgacgccg acauccagga caccaucaac     2220

gagcucuaca ccagcccgca gaacaagaag gccauccgcg aggugaugcu gguccuggac     2280

gacaucaaga acgcgaugca cgggcagacc cccuccugga ucuucgugga ggccgcccgg     2340

ggcgggggcg uggccggccg ccggacgcag agccgcagcu cccagaucgu cgaggccuac     2400

aaggggaccg cgaaggagau cgugagcgag aaggugcagc acgagcucaa cgagaagauc     2460

aaggccaagg ccgacuucaa cacccggcug guccuguacu uccuccagaa cggccgcgac     2520

cuguacacca acgaggccau caacaucgac cggcuguccg aguacgacau cgaccacauc     2580

cucccccaga gccuggugaa ggacgacucc cuggacaacc gcgugcucac gagcgcccgg     2640

aucaaccgcg agaagaacga caccuucgcg uccgagaagu ucgggcggaa gaugggcgcc     2700

caguggcgcg agcugcaccg gaacggccug augacccagc gcaagcucaa gcaccuccug     2760

augcggcccg acgagaucag caagcacgcc accgacuuca ucaaccgcca gcuggucgag     2820

acgcgccagg ugaucaagcu gguggaggag cugaucucca gcgaguaccc ggccgccucc     2880

aucgucgcgg ugaaggccaa ccucacccac caguuccggc agaccuucaa cuuccccaag     2940

cugcgcgagg ugaacgacua ccaccacgcc uucgacgcca gccugaccgc guucaucggg     3000

auguaccucc ugaagcagua ccccaagcug gagcgguucu ucgucuacgg caaguucgcc     3060

aagcagccga ucaaccucac gcgcuucaac aucauccgga agcuggccgu ggccgagaag     3120

cccaucgcca acaucgagac cggggagauc cugugggaca agaccgcgga caucaaguac     3180

uucgagaagc ucuacaacua caagcgccug cuggugaccc acgagguccg ggagaacuac     3240

ggcgccaugu ucaagcagac gcuguucaag gccuccgaca acaagagcaa gacccuggug     3300

cccaagaaga accacaugga gaccuccgug uacgggggcu acagcaacca ggagaccgcc     3360

uaccuggcca ucguccgcau ccccuucaag uccggcuuca aguucaucgu gguggggauc     3420

ccgacgcgga uggucgcgaa gaucaagcac uaccagagcc ucggcgccac ccugaagcag     3480

gccacccaca aggugaucga gcccaaguuc accaagauca gccgcaagac gaagcagacc     3540

gugaucuccg acuacgaggu cgugcugccc aagguguacc ucgaccaggu cgugcgggac     3600

caggugaagg ggcagaugua ccgcuucagc cugggcuccg acaaggagua ccacaacguc     3660

caggagcugu accucccccu gagcauccag caggccuucg uggggcacua cgacgagucc     3720

gacgaccagc ggagcaacga ccuggugaag gucuacgacg ccgcgcucaa gcagcugcag     3780

cgcuacuucc cgcugcaccu cucccggaac uucgaccagg uggccaacca ggcccaccag     3840

agcuucgagg gccugaagaa caacguccag aaguccgaca agcagcuggg caagaaggag     3900

gugcucaaca gccuguucgu ggggcugcac gccaccgcca cccgcuccaa ccucagcguc     3960

cugggcaugu ccaaggacuu cgggcggcug cagagccgcu ccggcaucac gcucaccgac     4020

caggcggaga ucaucuacca gagccccacc gggcuguucg agcggaaggu ggcccugaag     4080

gaccucuga                                                             4089


<210>  1910
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1910
augaccaagc ugggcaagcc cuacgggauc ggcguggaca ucgggagcaa cuccaucggc       60

uucgccgccg ucgacgagaa cagccaccuc auccgccuga aggggaagac cgugaucggc      120

gcgcggcugu ucgaggaggg caaggccgcc gccaagcgcc gggccgggcg cacgacccgg      180

cgccggcucu cccgcaaccg guggcgccug agcuuccugc gggacuucuu cgagucccac      240

aucaccccga ccgaccccaa cuucuucaug cgccagaagu acagcgagau cucccccaag      300

gacaaggcgc gguacaagua cgagaagcgc cucuucaacg accggacgga cgccgaguuc      360

uaccagcagu acagcaccau guaccaccuc cgcaaccggc ugaugaccga ccccucccgc      420

gccgacgugc gggagaucua cuucgccauc caccacaucc ucaagagccg cggccacuuc      480

cugaccccgg gggacgcgaa ggacuucaac acgaacgacg ucgcccugga cgagaucuuc      540

cccgcccugc aggacgccua cgcccaggug uaccccgacc ugggcaucac cuucgacgag      600

gacaaggcga acgaguucaa gaccauccug cucaacgaga ucgccaccuc caucgacacg      660

cagcgggccc uggugaagcu gcuccuggcc gaggaggacg acaaggacau ccugaagcag      720

cagaagcagg uccucaccga guucgccaag gcgguggugg ggcugaagac caagcugaac      780

cucgcccugg gcaccgaggu cgacagcucc gaggccacgg ccuggaacuu cagccugggc      840

cagcucgacg acaagugggc cgggaucgag uccgcgauga ccgacgaggg caccgagauc      900

cuggaccaga uccgcgaccu guaccgcgcc cggcuccuga acgggaucgu gcccaccggc      960

aagacgcuga gccaggccaa gguggacgac uacacccagc accacgagga ccuccagcug     1020

uucaaggccu accugaagca gcucgaggac gacggcaccg ccaaggcgau ccgccagcug     1080

uacgaccggu acaucgacgg ggacgacgcc gccccguucc ugcgcgagaa cuucgucaag     1140

gcccucacca aggacgugac ggcgcacccc aacaccaagu cccccgagcu gcuggagcgg     1200

cucgcccagc ccgacuucau gcugaagcag cgcaacaagg ccaacggcgc caucccggug     1260

cagaugcagc agcgggagcu ggaccagauc aucaagaacc aggccgucua cuacgacugg     1320

cucgcggccc ccaaccccgu ggagaagcac cgcaagagca ugcccuacca gcuggacgag     1380

cugcucaacu uccggauccc guacuacgug gggccccugg ucaccgccaa ggagcagaag     1440

gccgcccagg gcgggguguu cgcguggaug gugcgcaagg accccgaggg caacaucacc     1500

cccuacaacu ucgacgagaa ggucgaccgg gaggccuccg ccaacacguu cauccagcgc     1560

augaagacca ccgacaccua ccugaucggc gaggacgugc ugccgaagca gagccuccug     1620

uaccagcggu acgaggugcu gaacgagcuc aacaacgucc gcgugaacaa cgagaagcug     1680

agcaucgagc agaagcagca ggucauccgg gagcuguucg agcgccacaa cuccgugacg     1740

aucaagcagu ucgccgagaa ccuccgggcc cacggggacu acgcgcacau ccccgagauc     1800

cgcggccugg ccgacgagaa gcgguuccug agcucccuca gcaccuaccg ccagcugaag     1860

ucccugcucc ccgaggccau cgacgacccc gccaagcagg cggacaucga gaacaucauc     1920

gccuggagca ccguguucga ggacgccgcc aucuucaaga ccaagcugaa ggagaucucc     1980

uggcugggga gcgaggccau cacgaagcuc uccaacaucc gcuaccgggg cugggggcag     2040

uucagccgca aguuccugaa cggccugacc cucggcaacg ggcacaccau cauccaggag     2100

cugcugcucu ccaccaacaa ccugaugcag auccugaccg acgagacgcu ccagaagaag     2160

augaccgagc ugaacgcgga caagcugaag accgccaaca ucaacgacgc caucgacaac     2220

gccuacacca gcccguccaa caagaaggcc cuccggcagg uccugcgcgu gguggacgac     2280

aucaagcggg cggccgacgg ccaggacccc agcuggcugu acgucgagac ggccgacggg     2340

ggcgggaccc ccggcaagcg cacccgggcc cgccagcacc agcuccagga gaucuacgcc     2400

aacgcggccc acgagcugau cgacaccgcc gugcggggcg agcuggagga caagaucucc     2460

gacaaggccg acuucaacga ccgccucgug cuguacuuca ugcagggcgg gcgggacauc     2520

uacacggggg cgccccugaa caucgaccag cucagcuccu acgacaucga ccacauccug     2580

ccgcagagcc ugaucaagga caacucccuc gacaaccgcg uccuggugca cgccaucauc     2640

aaccgggaga agaacgccac cuucgccagc accaucuacg cccagaagau gaacgcgacc     2700

uggcgccagu ggcacgaggc cggccugauc agcggccgga agcuccgcaa ccugcagaug     2760

cggcccgacc agaucgacaa guacgccucc ggguucgugg cccgccagcu ggucgagacg     2820

cggcagauca ucaagcucac cgagcagauc guggccgcgc aguaccccga caccaagauc     2880

aucgccguga aggccggccu gagccaccag cugcgcgagg agcucgaguu cccgaagaac     2940

cgggacguca accacuacca ccacgccuuc gacgccuucc uggcggcccg caucgggacc     3000

uaccugcuca agcgcuuccc caaccugcag gccuucuuca cguacggcaa guucaccaag     3060

gccgacguga agaagcugcg gggguucaac uucauccgcg acaucaccca cgccgaggac     3120

aagaucgugg cgaaggacac cggcgagguc gugugggaca agcagcggga cguggacgag     3180

cucgaccgca ucuacaacuu caagcggaug cugaucacgc acgagguccg cuucgagacc     3240

gccgaccugu ucaagcagac cguguacggc gcccgggacu ccaaggaggc cgggggcagc     3300

aagcagcuca uccccaagaa gaagggguac cccguggaca ucuacggcgg guacuuccgc     3360

gagaacaccg cguaccuggc cgucgugaag gugacgaaga agaccgagac caucuucaag     3420

gucgugaaga ucgccaccuc ccagguggcc gcccugaaca aggcgcggag ccgcuccacg     3480

gccgaggagc ucagcguccu gaccgagcug cucaagccga aguucuccaa ggugggcaag     3540

aacggcaaga ucaccgacac ccccuucgag gugguccugc cccgggugcc ccgcgagcag     3600

cuguucuaca acgccaagua cggguucuuc auggugaaca gcgacacgau guuccacaac     3660

uuccaggaga ucugggucuc ccggagcgac cagaagaucc uccagcagau ccgcaaggcc     3720

aagaucgacu acccgaacgu ggaccaggac cuggacaacc uguuccacaa ccucgccgac     3780

cagaucguga aguacuucga ccuguacucc aucgcgggcu ucaaggagaa gaucagccag     3840

ucccagaaca ccuucaacga ccuggccguc gacgacaccg accagagcgu ggggaagauc     3900

accgugauca acgagcuccu gaagggcgcc caggccaacg ggaugacggg cucccugaag     3960

guccucaaga ucagcacccc cuucggcuuc acccaggaca agagcggggu gcugaccaag     4020

gacuccagca ucaucuacca gucccccacc ggccuguucg agcggagcgu gcgccucacg     4080

gaccuguga                                                             4089


<210>  1911
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1911
augaccaagc ugggcaagcc cuacgggauc ggcguggaca ucgggagcaa cuccaucggc       60

uucgccgccg ucgacgagaa cagccaccuc auccgccuga aggggaagac cgugaucggc      120

gcgcggcugu ucgaggaggg caaggccgcc gccaagcgcc gggccgggcg cacgacccgg      180

cgccggcucu cccgcaaccg guggcgccug agcuuccugc gggacuucuu cgagucccac      240

aucaccccga ccgaccccaa cuucuucaug cgccagaagu acagcgagau cucccccaag      300

gacaaggcgc gguacaagua cgagaagcgc cucuucaacg accggacgga cgccgaguuc      360

uaccagcagu acagcaccau guaccaccuc cgcaaccggc ugaugaccga ccccucccgc      420

gccgacgugc gggagaucua cuucgccauc caccacaucc ucaagagccg cggccacuuc      480

cugaccccgg gggacgcgaa ggacuucaac acgaacgacg ucgcccugga cgagaucuuc      540

cccgcccugc aggacgccua cgcccaggug uaccccgacc ugggcaucac cuucgacgag      600

gacaaggaga acgaguucaa gaccauccug cucaacgaga ucgcgaccuc caucgacacg      660

cagcgggccc uggugaagcu gcuccuggcc gaggaggacg acaaggacau ccugaagcag      720

cagaagcagg uccucaccga guucgccaag gccguggugg ggcugaagac caagcugaac      780

cucgcgcugg gcaccgaggu cgacagcucc gaggccacgg ccuggaacuu cagccugggc      840

cagcucgacg acaagugggc cgggaucgag uccgccauga ccgacgaggg caccgagauc      900

cuggaccaga uccgcgaccu guaccgcgcg cggcuccuga acgggaucgu gcccaccggc      960

aagacgcuga gccaggccaa gguggacgac uacacccagc accacgagga ccuccagcug     1020

uucaaggccu accugaagca gcucgaggac gacggcaccg ccaaggccau ccgccagcug     1080

uacgaccggu acaucgacgg ggacgacgcg gccccguucc ugcgcgagaa cuucgucaag     1140

gcccucacca aggacgugac ggcccacccc aacaccaagu cccccgagcu gcuggagcgg     1200

cucgcgcagc ccgacuucau gcugaagcag cgcaacaagg ccaacggcgc caucccggug     1260

cagaugcagc agcgggagcu ggaccagauc aucaagaacc aggccgucua cuacgacugg     1320

cucgccgcgc ccaaccccgu ggagaagcac cgcaagagca ugcccuacca gcuggacgag     1380

cugcucaacu uccggauccc guacuacgug gggccccugg ucaccgccaa ggagcagaag     1440

gccgcccagg gcgggguguu cgccuggaug gugcgcaagg accccgaggg caacaucacc     1500

cccuacaacu ucgacgagaa ggucgaccgg gaggcguccg ccaacacguu cauccagcgc     1560

augaagacca ccgacaccua ccugaucggc gaggacgugc ugccgaagca gagccuccug     1620

uaccagcggu acgaggugcu gaacgagcuc aacaacgucc gcgugaacaa cgagaagcug     1680

agcaucgagc agaagcagca ggucauccgg gagcuguucg agcgccacaa cuccgugacg     1740

aucaagcagu ucgccgagaa ccuccgggcc cacggggacu acgcccacau ccccgagauc     1800

cgcggccugg cggacgagaa gcgguuccug agcucccuca gcaccuaccg ccagcugaag     1860

ucccugcucc ccgaggccau cgacgacccc gccaagcagg ccgacaucga gaacaucauc     1920

gcguggagca ccguguucga ggacgccgcc aucuucaaga ccaagcugaa ggagaucucc     1980

uggcugggga gcgaggccau cacgaagcuc uccaacaucc gcuaccgggg cugggggcag     2040

uucagccgca aguuccugaa cggccugacc cucggcaacg ggcacaccau cauccaggag     2100

cugcugcucu ccaccaacaa ccugaugcag auccugaccg acgagacgcu ccagaagaag     2160

augaccgagc ugaacgccga caagcugaag accgcgaaca ucaacgacgc caucgacaac     2220

gccuacacca gcccguccaa caagaaggcc cuccggcagg uccugcgcgu gguggacgac     2280

aucaagcggg ccgcggacgg ccaggacccc agcuggcugu acgucgagac ggccgacggg     2340

ggcgggaccc ccggcaagcg cacccgggcc cgccagcacc agcuccagga gaucuacgcc     2400

aacgccgcgc acgagcugau cgacaccgcc gugcggggcg agcuggagga caagaucucc     2460

gacaaggccg acuucaacga ccgccucgug cuguacuuca ugcagggcgg gcgggacauc     2520

uacacggggg cgccccugaa caucgaccag cucagcuccu acgacaucga ccacauccug     2580

ccgcagagcc ugaucaagga caacucccuc gacaaccgcg uccuggugca cgccaucauc     2640

aaccgggaga agaacgccac cuucgccagc accaucuacg cccagaagau gaacgccacc     2700

uggcgccagu ggcacgaggc gggccugauc agcggccgga agcuccgcaa ccugcagaug     2760

cggcccgacc agaucgacaa guacgccucc ggguucgugg cccgccagcu ggucgagacg     2820

cggcagauca ucaagcucac cgagcagauc guggccgccc aguaccccga caccaagauc     2880

aucgcgguga aggccggccu gagccaccag cugcgcgagg agcucgaguu cccgaagaac     2940

cgggacguca accacuacca ccacgccuuc gacgccuucc uggccgcgcg caucgggacc     3000

uaccugcuca agcgcuuccc caaccugcag gccuucuuca cguacggcaa guucaccaag     3060

gccgacguga agaagcugcg gggguucaac uucauccgcg acaucaccca cgccgaggac     3120

aagaucgugg ccaaggacac cggcgagguc gugugggaca agcagcggga cguggacgag     3180

cucgaccgca ucuacaacuu caagcggaug cugaucacgc acgagguccg cuucgagacc     3240

gcggaccugu ucaagcagac cguguacggc gcccgggacu ccaaggaggc cgggggcagc     3300

aagcagcuca uccccaagaa gaagggguac cccguggaca ucuacggcgg guacuuccgc     3360

gagaacaccg ccuaccuggc ggucgugaag gugacgaaga agaccgagac caucuucaag     3420

gucgugaaga ucgccaccuc ccagguggcc gcccugaaca aggcccggag ccgcuccacg     3480

gcggaggagc ucagcguccu gaccgagcug cucaagccga aguucuccaa ggugggcaag     3540

aacggcaaga ucaccgacac ccccuucgag gugguccugc cccgggugcc ccgcgagcag     3600

cuguucuaca acgccaagua cggguucuuc auggugaaca gcgacacgau guuccacaac     3660

uuccaggaga ucugggucuc ccggagcgac cagaagaucc uccagcagau ccgcaaggcc     3720

aagaucgacu acccgaacgu ggaccaggac cuggacaacc uguuccacaa ccucgccgac     3780

cagaucguga aguacuucga ccuguacucc aucgccggcu ucaaggagaa gaucagccag     3840

ucccagaaca ccuucaacga ccuggcgguc gacgacaccg accagagcgu ggggaagauc     3900

accgugauca acgagcuccu gaagggcgcc caggccaacg ggaugacggg cucccugaag     3960

guccucaaga ucagcacccc cuucggcuuc acccaggaca agagcggggu gcugaccaag     4020

gacuccagca ucaucuacca gucccccacc ggccuguucg agcggagcgu gcgccucacg     4080

gaccuguga                                                             4089


<210>  1912
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1912
augaccaagc ugggcaagcc cuacgggauc ggcguggaca ucgggagcaa cuccaucggc       60

uucgccgccg ucgacgagaa cagccaccuc auccgccuga aggggaagac cgugaucggc      120

gcgcggcugu ucgaggaggg caaggccgcc gccgaccgcc gggccucccg cacgacccgg      180

cgccggcuca gccgcaaccg guggcgccug uccuuccugc gggacuucuu cgagagccac      240

aucaccccga ccgaccccaa cuucuucaug cgccagaagu acuccgagau cagccccaag      300

gacaaguccc gguacaagua cgagaagcgc cucuucaacg accggacgga cgcggaguuc      360

uacaaguacg agaacugccc caccauguac caccuccgca accggcugau gaccgacccg      420

agcaaggccg acgugcgcga gaucuacuuc gccauccacc acauccucaa gucccggggg      480

cacuuccuga cccccggcga cgccaaggac uucaacacga acaaggucgc ggugaacaag      540

aucuuccccg cccugcagga cgccuacgcc cagguguacc ccgaccucga caucaccuuc      600

gacgagaaca aggucaacga guucaagacc gugcugcuga acgagaaggc caccccgagc      660

gacacgcagc gcgcgcucgu gaaccugcug cucgccaagg agggggaccg ggacauccug      720

aagcagcaga agcaggcccu gaccgaguuc gccaaggccg ucgugggccu caagaccaag      780

cugaacgugg cgcuggggac cgaggucgac uccagcgagg ccacggccug gaacuucucc      840

cucggccagc uggacgacaa guggaccggc aucgagagcg ccaugaccga cgaggggacc      900

gagauccugg accagauccg cgaccucuac cgcgcccggc ugcugaacgg caucgugccc      960

gcggggaaga cgcucuccca ggccaaggug gacgacuacg cccagcacca cgaggaccug     1020

gagcuguuca aggacuaccu caagcagcug gaggacgacg gcaccgccaa ggccauccgc     1080

cagcuguacg accgguacau cgacggcgac gacgcgaagc ccuucguccg cgaggacuuc     1140

gugaaggccc ucaccaagga ggugaccgcc caccccaacg ccaagagccc ggagcugcug     1200

gagcggcucg cgcagcccga cuucaugcug aagcagcgca acaaggccaa cggggccauc     1260

cccguccaga ugcagcagcg ggagcuggac cagaucauca agaaccaguc cguguacuac     1320

gacuggcucg ccgcccccaa cccgguggag aagcaccgca agagcaugcc cuaccagcug     1380

gacgagcugc ucaacuuccg gauccccuac uacgucggcc cccuggugac ggcgaaggag     1440

cagaaggccg cccagggcgg gguguucgcc uggauggucc gcaaggaccc ggacgggaac     1500

aucacccccu acaacuucga cgagaaggug gaccgggagg ccagcgcgaa caccuucauc     1560

cagcgcauga agaccacgga caccuaccug aucggcgagg acguccuccc caagcagucc     1620

cugcuguacc agagcuacga ggugcugaac gagcucaaca acgugcgggu caacgacaac     1680

aagcuguccu ucgaccagaa gcagcagguc cugggcgagc uguucgagcg ccacaacacc     1740

gugaccauca agcaguucgu ggacaaccug cuggcccacg gggacuacga ccggaucccc     1800

gaggugcgcg gccucgccga cgagaagcgg uuccugagcu cccugagcac guacaaccag     1860

cucaagggga uccugcacga ggccaucgac gacuccgaca agcagaccga caucgagaac     1920

aucaucgccu ggagcaccau cuucgaggac aaggcgaucu ucaagaccaa gcugaacgag     1980

aucuccuggc ucaacgccgg caugaucaac agccuggcca acauccgcua ccgggggugg     2040

ggccaguucu cccacaagcu gcucaacgag cugacgcuga gcaccggccg caccguguuc     2100

gaggagcucc ugaucuccag ccacaaccug augcagaucc ucaccgacga gguccugaag     2160

cagaagaucg ccgagcugaa cgcgcacgag cucaaggccu ccgggaucua cgccgccauc     2220

gacgacgccu acaccagccc guccaacaag aaggcgcugc gccaggugcu gagcgugauc     2280

gacgacauca cgaacgccgc cggccaggcc cccaacuggc ucuacaucga gaccgccgac     2340

ggggacggcg uccccgggaa gcggaccaac ucccgccagc ggaagaucca ggagaucuac     2400

gcgaacgccg cccaggagcu gaucaccggc agcgugcgcg gcgagcugga ggacaagauc     2460

gccagccggg ccgacuucac ggaccgccuc gugcuguacu ucaugcaggg cgggcgggac     2520

aucuacaccg ggaagccccu gaacaucgac aaccucucca gcuacgacau cgaccacauc     2580

cugccgcagu cccugaucaa ggacaacagc cucgacaacc gcguccuggu gcacccccug     2640

gugaaccggg agaaggacac caucuucgcg uccacccgcu ucgccaagca gaugaacggc     2700

auguggcggc aguggcacgc cgccggccuc aucagcuccc gcaagcugcg gaaccugcag     2760

augcgccccg acgagaucga caaguacgcg agcggguucg ucgcccggca gcucguggag     2820

acgcgccaga ucaucaagcu gaccgagcag aucguggccg accaguaccc ggacaccaag     2880

aucaucgccg ucaaggccgg ccuguccagc cagcuccgga aggagcugga cuuccccaag     2940

aaccgcgagg ugaaccacua ccaccacgcg uucgacgccu uccuggccgc ccggaucggg     3000

accuaccucc ugaagcgcua ccccaaccug gagcccuucu ucacguacgg caaguucaag     3060

aagaccgagg ugaagaagcu caaguccuuc aacuucaucc gggacaugac ccacgccaag     3120

gacaagaucg ucgcgaagga gaccggggag aucguguggg acaacgccag cgacaucaac     3180

gagcuggacc gcaucuacaa cuucaagcgc augcugauca cgcacgaggu gcgguucgag     3240

accgccuccc ucuucaagca gacccuguac gccgccaaga acagcaagaa ccgcggcggc     3300

ucccggcagc ugaucccgaa gaagaagggg uaccucgucg acaucuacgg cggguacacc     3360

caggagacgg gcagcuaccu guccguggug cgccugacca agaaggcgau guacgccguc     3420

gugaagguga gcacccggga cgccgccaag cucgccgucg ccaagagcau cuccgagcag     3480

aaggagaacg agacccugaa gaagaucauc gacgggaagc ugagcaagac guccaagaag     3540

ggcaagacca cccaccagcu guucgagauc gugcuccccc gcgugggcca gaagacccug     3600

uucaagaaca gcaaguacaa ccaguuccuc gucaacuccg acacguacau gcacaacuac     3660

caggagcugu ggaugccccg ggaguaccag cgcaugugga aggacauccu gcucagcaac     3720

cacggggacg cgcagcugga gggccagcug gaccagaucu ucaaguucau cgugucccag     3780

gugaacagcu acuucaaccu cuacgacauc aaccaguucc ggaagaagau cgccgacgcc     3840

accgacaagu ucgcgaagcu gcccacccac gacaccgaca acgcccgcgg gaagaucgcc     3900

acgaucggcc agcugcucau cgggcugcag gccaacgcca ugcgguccga ccugcgcaac     3960

cucgacauca agaccccguu cggccugcug cagaucggca ccgggaucac ccucgaccug     4020

gacacgagca ucaucuacca gucccccacc ggccuguucg agcgggaggu cccccucacc     4080

gaccuguga                                                             4089


<210>  1913
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1913
augaaccagg agcccuacgc caucggccug gacaucggga ccagcucccu cggcugggcc       60

aacgcggaca uggacgggua caucgcccgc gugaagggcc agaccgggcu gggcguccgg      120

cuguucgagg agggccagcc ggccgccgac cgccggagcu uccgcacgac ccggcgccgg      180

cucucccgcc ggcgcuggcg gcugcgccug cuccgggaga ucuucgacga gcccaucagc      240

uccaucgacc ccaacuucuu cgcccgccag aaggcgagcu ccaucagccc caaggacgac      300

cgguucgaga ccaccuacca gcuguucaac gacaagacgg accaggaguu cuacuccaag      360

uacccgacca ucuaccaccu ccgcugggcc cugaugaccg agcaccggaa guucgaccug      420

cgcgagaucu accuggccgu gcaccacauc gugaaguacc gggggaacuu ccuccgcagc      480

ggcaacccca aguccuucaa gcccagcgag cuggacuucu cccagcgguu caaggagguc      540

aacgggcugu ggcaccucau cuucgacgag guggcgcccc agcugccgac cgacaaccug      600

gaggagauca cgaagcucgu gcuggacacc acccgcaccc gcgccgaccg gcagcgcgag      660

agcgucaagc ugcucauggc cgccacggac aaccacaagg acuggaagcc cuaccugacc      720

gaguucacca aggccauccu gggccucaag accaagcugu accugcucac ggggguggac      780

guggccaagg cggaccagaa gaccuggacc uucucccugg aggacaucga ggaccaccgg      840

gccgaccugg agaccgccuu cgacgaccgc agccucgagc ugaucgagaa gcuggucgac      900

cuccaggccg ccauccagcu gacggagauc augcccuccg gccaguacuu cagccagucc      960

augaucuacc gguacgacca gcacaaggag caccuccgcu ggcugaagga cuacgcggag     1020

cagcagaccg acgccaacaa gcggaaggcc gugcgccugg ccuacgacca cuacaucgag     1080

ggcgacgcca aggggaagcc cgagaucgcg gacgacuucu acaaggagcu gaaccggauc     1140

cucaagggcg acaccagcga gcuggccaag aagaucaacc acgagaucgc ccuggagcac     1200

uucaugccga agcagcgcac caaggagaac ggggugaucc ccuaccagau ccaccagcag     1260

gagcucgaca gcaucaucga caaccagaag aaguacuacc aguuccuggg cgaggacaac     1320

cccgucgugu cccaccacgg cagccagccc uacaagaucg acgagcuggu guccuuccgg     1380

gucccguacu acguggggcc caugaucacg gccgaccagc agaagaagac cgccgcgggc     1440

caguucgccu ggauggugcg caaggacagc ggggacauca cccccuggaa cuucgacgac     1500

aaggucgacc ggaccgccuc cgccacgaac uucauccagc gcaugaagac caccgacacc     1560

uaccucaucg gcgaggacgu gcugccccug cagagccuca cguaccagaa guucauggug     1620

cugaacgagc ugaacaacau ccggaucaac ggggagcugc ucacccgcga gcagaagcag     1680

cggaccuaca acgacgucuu caagggccac ggcaccguga acgugaaguc ccugaucaag     1740

agccuggagu gccacuccga ggucacgggg gcgaucaagg uggagggccu cgccgacccg     1800

aagcgcuuca acagcgcccu gaccaccuac cgggacuuca agaagauccu gcccgucgcc     1860

aucgaccagc cccagcgcca ggccgacauc gagcgcauca ucaacugguc caccaucuuc     1920

gaggacgcgg acaucuucaa ggccaagcuc caggagaucg acuggcugac cgacacgcag     1980

cgggaccgcc ugagcaccaa gcgguaccgc ggguggggcc ggcucuccgc caagcugcug     2040

cucaacauca aggacaccca ggggcugagc aucauggacc agcuguggaa cacccagcac     2100

aacuucaugc agaucgugca cgagcccgac uacgccgaga agaucgagaa gacgaacgcc     2160

aagggccuca agcagcagga caucuucgag accaucaacg agauguacac cuccccgcag     2220

aacaagaagg cgcugcgcca gauccuggcc gugguccggg acauccagaa ggcccaccac     2280

ggccaggccc ccagccgcau cuucaucgag gccgcgcgcg gggccgagca gaacccccag     2340

cggacccggc agcgccagaa gcagcucgcc gaccuguucg ccgaccaggc gaaggagauc     2400

gugaacgaga cgguggccca ggagcugaac gacgagaccg acaacaaggc ccgguucacc     2460

gaccgccuca ugcuguacuu ccugcagaac ggccaggaca uguacaccgg ggagaagcuc     2520

aacaucgaca agcuguccac guacgacauc gaccacaucc ugccccagag ccucgucaag     2580

gacgacuccc uggacaaccg ggugcuggug agcaaggucg agaaccucaa gaagaacgac     2640

cacuucgcca gcgagcugua cgcccgcaag augcagggca ccugggagcu guggcggucc     2700

gcggggcuca ucagcgcccg caagcugaag caccuccuga ugcggccgga cgagaucaac     2760

aaguacgcca ccggcuucau ccgccggcag cugguggaga cccgccaggu gaucaagcug     2820

gucacggacg ugcucuccaa cgaguacgac agcgacaaga ccaagaucau cuccgugaag     2880

gccggccuga gccaccagcu gcgcauggag cucgaccugc ccaagguccg ggagcugaac     2940

gacuaccacc acgccuucga cgcguuccuc gccgugcgca ucgggaccua ccugcugaag     3000

cgguacccca agcucgagcg cuucuucgug uacggccagu accaggucgc cccgcagcug     3060

gaccugcggc gcuucaacuu cauccacgac cucguguccg ggaagcagga gaagaucgug     3120

gccgaggaga ccggcgagau cgucuggaac cgggagaagg acgugagcua caugaagcac     3180

cuccuguccc ugaagcaccu ccuggugacg cacgagguca aggaggacca cggcgcccug     3240

uucaaccaga ccaucuacaa ggcgaagaag gacaacggga acgugggcgg gaagaugaag     3300

cugcugccca ccaagagcaa ccgccccacc gagaucuacg gcgggcacuc cagccaggcc     3360

aucgccuacc ucgccaucgu gcgggucaac ggcaaggagc ccuccuaccg cgugaugggc     3420

gugccgaucc ggauccugga ccagcugaag caggcgaaga agcaggggga guacgccgag     3480

cgccucgccc ugaagcaguu cuucacgccc gacuucacca agggcaaggg gaagaucguc     3540

agcgacuucg acguggugau cccccacauc ccccugcucc agccggucau cgacgagauc     3600

uccggccaga gccaccgguu cgcccugggg accaacaccu acuaccgcaa ccugcagcag     3660

cucauccuga cgcuggccga ccagaaggug cucaucucca aggagaccgg cguggacgag     3720

gagaagaagc uggucgaggu guacagccac aucugccagc aggucaugaa guucuucccc     3780

cuguacgacc ggaacaaguu ccgcgagcuc cugguggaca gccgguccca cuucgagcag     3840

cugcccaucg ugaaccgcua cagcgacgcg ggcaagcucg agaucgccgg gaagcggggc     3900

auccugcagc agaucauggu ggggcugcac gccaacgcca cccccggcga ccucaaggag     3960

cuggggguca agaccccguu cggcaagcuc caggugcccg gcgggaucaa gcugacggag     4020

aacgccaagc ugaucuacga gucccccacc ggccuguuca agcgcgaggu gaagaucacc     4080

accgaguga                                                             4089


<210>  1914
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1914
augaagaaga aggccgagaa gaucuaccug ggccucaacg uggggaccaa cagcaucggc       60

uacgccguca ccgacgagca guaccgccug cugaaguuca acgggaacga cgcguggggc      120

uccaucgugu ucgacgccgc cagcuccaac gaggaccggc gcaaccaccg gcucgcccgc      180

cggaaccugg accgccggca gcagcgcauc guguggcugc aggagaucuu cgccaaggag      240

aucagcaagg ucgacgaccg guucuucauc cgccucuccg agagcuuccg guggcgcgag      300

gacgugcagg accgguacgu guucuucaac gacaaggagu acacggacgu ccaguacaug      360

aaggaguacc ccaccaucca ccaccugauc ugcgagcuga uggacaacaa gaccccgcac      420

gacgugcgcc ucguguaccu ggcgugcgcc uggcugauca cccaccgggg gcacuuccuc      480

aacaaccuga acgucgagaa gcuggacgag aucauggacu ucuccagcgu guaccagaac      540

uucaucaacu acuucacgga gaacggcuac aagcgccccu ggggcgagau cgacgugucc      600

gcccucagcg agguccugaa gcagaagacc gggaucaccg ugaagaacaa ggagcugcag      660

aacauccucc uggagggcaa gaagcccgag aaguccggga ccgaggaguu ccccuucagc      720

caggacucca ucauccggcu gcucgccggc gggcagugca agcugaagga cguguucugc      780

aaggaggagu accuggaccu cggcagcguc ucccugggca uggacgagga gaaguucgac      840

gagaucagcg gguccaucgg cgaggacuac gaccugcuca ucgcgcugcg cgggcuguac      900

gacuggagcg ugcucgccaa ccugcugaac ggccacuccg agacgaucag cauggccaag      960

gugcagaugu acgagcagca cggcaaggac cuccagaucc ugaaguacuu cguccggaag     1020

uacgugccgg acaaguacaa cgagguguuc cgcaaggcca aggccgacaa cuacgucgcg     1080

uacaccgggc acauggacaa ggacaccgcc agccagauca agaagaaggc caaggccgag     1140

gaguucucca aguacgugcu gaagcucaug agccccauca agcccgagaa gaccgaccug     1200

caguccuacg aggacaugug cgagcggcug aagcucaacc gcuuccugcc caagcagaag     1260

aacacggaca accgcgugau cccgcaccag cuguacgagu acgagcucau ccagauccug     1320

aagaacgcca gcgucuaccu gcccuuccuc aacgacacca acaacggcau cuccgaggcg     1380

gacaaggugc ugagcgucuu ccgguacaag auccccuacu acguggggcc ccugaacgag     1440

gaguccagcu ucgccuggau cucccgcaag cagggcaaga ucaccccgug gaacuucaag     1500

gagauggugg acgaggacgc cagcgagaac gccuucaucc ggcgcaugac caacaagugc     1560

acguaccucc ccaccgagga cguccugccc aaggacuccc ugugcuacca gaaguucaug     1620

gugcugaacg agaucaacaa ccuccagauc gacgggcuga agauccccgu gaaggccaag     1680

cagggcaucu accacgagcu guucgagaag aacaagaagg ucaagcggaa ggacaucgag     1740

gaguaccuca uccgcaacaa cuaccuggac aaggacagca ccgagcugau cuccggcauc     1800

gacgagcaga uccacagcgg gcucuccagc uacugcgcgu ucuucaaccu gcugaacaag     1860

aagauccucu ccgaggacga cguggagcgg aucaucgagc gcgccagcua cgccgaggac     1920

aagucccggg ugaucaagug gcugcgcaag gaguacccgg gccugagcga ggacgacauc     1980

cgguacaucg cccgcaucaa gauccgggac uucgggcgcc ucagcaagac cuuccugacg     2040

ggccugaagg ggugcgacgu cuccaagggc uucgacggcc aggacgugga gaugaucagc     2100

auccuccaga ccauguggga gaccaacgac aaccugaugg agauccuguc cgacaaguac     2160

accuuccucg aggcccugga ggcguucacg caggaguacu acaccgggaa gaagaagucc     2220

cugaaggagc ggcucgacga gaugcgcgug agcaacagcg uccggcgccc cguguaccgg     2280

acccuggccg uggucaagga caucgagaag gccuucggca agcccaacaa gaucuucgug     2340

gagaugaccc gcgggggcca ggccgaccag aaggggaagc gcaucaaguc ccggaagcag     2400

cagauccugg accucuacga caagugcaag gacgacgugc acgaccugau guggcagcug     2460

gagagccucg gcgaguacgc ggacucccgc cugcagagcg accggcugua ccucuacuuc     2520

augcaguucg gcaagugcgc cuacuccggg gcccacaucg gcuucgagca gcugauggcc     2580

gggagcgucg aguacgacau cgaccacauc uacccccagu ccuacgugaa ggacgacagc     2640

cugaacaaca aggugcucgu ccuguccgac cugaacggca cgaaggacaa caucuacccg     2700

gugagccccg agauccgcgg caagaugcag gggcucuggu ccugguggaa ccacgugggc     2760

gccaucagcg acgagaagua caagcggcug auccgcagcg cgcccuucac cgacgaggag     2820

aagauggggu ucaucaaccg gcagcugacc gagaccuccc agagcacgaa guucaucgcc     2880

gaccuccuga aggagcgcuu ccccgacgcc gaggucgugu acgugaaggc cggccugguc     2940

uccgacuucc ggcacgaguu cgaccucccc aagagccgcu ccuacaacga ccugcacaac     3000

gccgcggacg ccuuccugag cgugguggcc gggaacgucu acgacaugcg guucuccaag     3060

aagugguucc gcaucgacga gaaguacagc gugaagguga agaccaucuu cacccacgag     3120

gucaagugcc ggggcgacau caucugggac ggcaugccga ugcucgagaa gguggaggac     3180

accgcccgca agaacacggc ccacuucgug aaguacgcgg ccuucaagac cgggggccug     3240

uucgaccaga accccgucaa gaaggggacc ggccugaucc cccucaaggc cgggcugccg     3300

accgagaagu acggcggcua caacaaggcc gggaucaugu uccugauccc cgugcgguac     3360

aacaucggca agaaguccga gauccucauc cugcccgugg agcugaugca cgggaagcac     3420

uuccucgagg acaaggucuu cgcgcgcaac uacaccuuca cgcggcugga gaacauccug     3480

ggcaagaagg ugggggaggu gagcuucccg augggcaugc gccccuggaa gaucaacacc     3540

augcucuccu ucgacggcuu ccgggucugc cugaccggga ccagcggccg cgggaagugc     3600

cugguggccc agcccgugau gcaguucucc gccgacgagc acuggcgguu cuacgucaag     3660

cgccucgagc gcuucgugga gaagaucggc aucaaccccg accugcugua cgacaaggac     3720

cacgacaagg ugagcaagga cgagaaccuc aagcuguacg agcuguacgu cgacaagcuc     3780

cagaacacga ucuacaagaa gcgggugaac uccccggugc agacccugcu gaaggggaag     3840

gaggaguuca agaagcucag caucgaggag cagugccagg uccugcugaa cauccagucc     3900

guguucggcc gcaugaccgg cgggugcgac cucacccuga ucggcgggag cgcccacucc     3960

gccgcgacgg gcagccugag caccaccauc uccaacugga agaagaagua cagcaccguc     4020

cggaucaucg accagucccc cagcggcaug ugggagaagu ccagcgagaa ccuccuggac     4080

uuccuguga                                                             4089


<210>  1915
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1915
augaccaagc ugggcaagcc cuacgggauc ggcguggaca ucgggagcaa cuccaucggc       60

uucgccgccg ucgacgagaa cagccaccuc auccgccuga aggggaagac cgugaucggc      120

gcgcggcugu ucgaggaggg caaggccgcc gccgaccgcc gggccucccg cacgacccgg      180

cgccggcuca gccgcaaccg guggcgccug uccuuccugc gggacuucuu cgagagccac      240

aucaccccga ccgaccccaa cuucuucaug cgccagaagu acuccgagau cagccccaag      300

gacaaggcgc gguacaagua cgagaagcgc cucuucaacg accggacgga cgccgaguuc      360

uaccagcagu accccaccau guaccaccuc cgcaaccggc ugcucaccga cccguccaag      420

gccgacgugc gcgagaucua cuucgccauc caccacaucc ucaagagccg ggggcacuuc      480

cugaccuccg gcuacgcgaa ggacuucaac acgaacaagg ucgcccugaa cgagaucuuc      540

cccgcccugc aggacgccua cgcccaggug uaccccgacc uggacaucac cuucgacgag      600

aacaagguga acgaguucaa gaccguccuc cuggacgaga aggcgacccc cagcgacacg      660

cagcgcgccc uggugaaccu ccugcuggcc gaggacgggg acaaggacau ccucaagcag      720

cagaagcagg uccugaccga guucgccaag gccguggugg gccugaagau gaagcucaac      780

guggcgcugg ggaccgaggu cgacuccagc gaggccaccg ccuggaacuu cucccugggc      840

cagcucgacg acaagugggc cggcaucgag agcgccauga cggacgaggg gaccgagauc      900

cuggaccaga uccgggaccu guaccgcgcg cgccuccuga acggcaucgu gccggccggg      960

aagacccugu cccaggccaa gguggacgac uacgagcagc accacgagga ccucgagcug     1020

uucaaggacu accugaagca gcucgaggac gacggcaugg ccaaggucau ccggcagcug     1080

uacgaccgcu acaucgacgg cgacgacgcc aagcccuucg ugcgggagga cuucgugaag     1140

gcgcugacca aggaggucac ggcccacccc aacgccaaga gccccgagcu ccuggagcgc     1200

cuggugcagc cggacuucau gcucaagcag cggaacaagg ccaacggggc gauccccgug     1260

cagaugcagc agcgcgagcu ggaccagauc aucaagaacc aguccgucua cuacgacugg     1320

cuggccgccc ccaaccccgu ggagaagcac cggcagagca ugccguacca gcucgacgag     1380

cugcugaacu uccgcauccc cuacuacgug ggcccccucg ucaccgccaa ggagcagaag     1440

gccgcgcagg gcgggguguu cgccuggaug guccggaagg accccgacgg gaacaucacc     1500

ccguacaacu ucgacgagaa gguggaccgc gaggccagcg ccaacaccuu cauccagcgg     1560

augaagacga ccgacaccua ccugaucggc gaggacgugc ugcccaagca gucccugcuc     1620

uaccagagcu acgagguccu gaacgagcug aacaacgugc gcgugaacga caacaagcuc     1680

uccuucgacc agaagcagca gguccugggc gagcuguucg agcggcacaa caccgugacg     1740

aucaagcagu ucguggacaa ccuccuggcc cacggggacu acgaccgcau ccccgagguc     1800

cggggccugg cggacgagaa gcgcuuccuc agcucccuga gcaccuacaa ccagcugaag     1860

gggauccucc acgaggccau cgacgacucc gacaagcaga ccgacaucga gaagaucauc     1920

gccuggagca ccaucuucga ggacaaggcc aucuucaaga cgaagcugaa cgagaucucc     1980

uggcugaccg ccggcaugau caacagccuc gcgaacaucc gguaccgcgg guggggccag     2040

uucucccaca agcugcugaa cgagcucacc cugagcaccg gccggacggu guucgaggag     2100

cugcucaucu ccagccacaa ccugaugcag auccugaccg acgaggugcu caagcagaag     2160

aucgccgagc ugaacgccca cgagcugaag gccuccggga ucuacgcggc caucgacgac     2220

gccuacacca gccccuccaa caagaaggcc cuccgccagg uccugagcgu gaucgacgac     2280

aucaccaacg ccgcgggcca ggccccgaac uggcuguaca ucgagaccgc cgacggggac     2340

ggcgugcccg ggaagcgcac gaacucccgg cagcgcaaga uccaggagau cuacgccaac     2400

gccgcgcagg agcucaucac cggcagcguc cggggcgagc uggaggacaa gaucgccagc     2460

cgcgccgacu ucaccgaccg gcuggugcuc uacuucaugc agggcgggcg cgacaucuac     2520

accggggagc cccugaacau cgacaaccug uccagcuacg acaucgacca cauccucccc     2580

cagucccuga ucaaggacaa cagccuggac aaccgggugc ucguccaccc gcuggugaac     2640

cgcgagaagg acacgaucuu cgccuccacc cgguucgcca agcagaugaa cggcaugugg     2700

cgccaguggc acgcggccgg ccugaucagc ucccggaagc uccgcaaccu gcagaugcgg     2760

cccgacgaga ucgacaagua cgccagcggg uucguggccc gccagcuggu cgagacccgg     2820

cagaucauca agcucaccga gcagaucgug gcggaccagu accccgacgc caagaucauc     2880

gccgugaagg ccggccuguc cagccagcug cgcaaggagc ucgacuuccc gaagaaccgg     2940

gaggucaacc acuaccacca cgccuucgac gcguuccugg ccgcccgcau cgggacguac     3000

cugcucaagc gguaccccaa ccuggagccc uucuucaccu acggcaaguu caagaagacc     3060

gaggugaaga agcugaaguc ccucaagagc uucaacuuca uccgcgacau gacccacgcc     3120

aaggacaaga ucguggccaa ggagacgggg gagaucgucu gggacaacgc guccgacauc     3180

aacgagcugg accggaucua caacuucaag cgcaugcuga ucacccacga ggugcgcuuc     3240

gagaccgcca gccucuucaa gcagacccug uacgccgcca agaacuccaa gaaccggggc     3300

ggcagccgcc agcugauccc caagaagaag ggguaccucg uggacaucua cggcggguac     3360

acgcaggaga ccggcuccua ccugagcguc gugcggcuga ccaagaaggc cauguacgcg     3420

guggucaagg ugagcacccg cgacgccgcc aagcucgccg uggccaaguc caucagcgag     3480

cagaaggaga acgagacgcu gaagaagauc aucgacggga agcuguccaa gaccagcaag     3540

aagggcaaga ccacccacca gcuguucgag aucguccugc cgcggguggg ccagaagacg     3600

cuguucaaga acuccaagua caaccaguuc cucgugaaca gcgacaccua caugcacaac     3660

uaccaggagc uguggaugcc ccgcgaguac cagcggaugu ggaaggacau ccugcucucc     3720

aaccacgggg acgcgcagcu ggagggccag cuggaccaga ucuucaaguu caucgucagc     3780

caggugaacu ccuacuucaa ccucuacgac aucaaccagu uccgcaagaa gaucgccgac     3840

gccaccgaca aguucgccaa gcugcccacc cacgacacgg acaacgcgcg ggggaagauc     3900

gccaccaucg gccagcugcu caucgggcug caggccaacg ccaugcgcag cgaccugcgg     3960

aaccucgaca ucaagacccc cuucggccug cugcagaucg gcaccgggau cacgcucgac     4020

cuggacaccu ccaucaucua ccagagcccg accggccugu ucgagcgcga ggugccccuc     4080

accgaccugu ga                                                         4092


<210>  1916
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1916
augcgcggca acuaccacau cgggcuggac aucggcacca gcuccaucgg gugggccgug       60

aucgacgaca acgccaaggu caugcgggcg aagggcaaga ccgccaucgg ggcccgccuc      120

uacaacgagg gccagacggc cgccgaccgg cgccuguucc ggaccacccg ccggcgccug      180

aaccggcgca aguggcggcu caagcugcug gacgagaucu ucgaccccua caucaccccg      240

guggacgcgg ccuucuucgc ccgccucaag cagagcaacc ugucccccaa ggaccagaac      300

cgggacugga cgggcagcau gcuguucccc gaccgcaccg accggaaguu ccacaaggac      360

uaccccacca ucuaccaccu ccgccacaag cugaugaccg aggaccacca guucgacauc      420

cgggagaucu accuggccau ccaccacauc gugaaguacc gcgggaacuu ccucaacacg      480

accccggucu ccagcuucuc cgugagcgac gugaaguucg acgagcaguu cgaccggcug      540

aacgagcugu acgcggccgu cucccccgac gagcccuucg ccaucaacgg cgacaacagc      600

aaggccaucg agacccuccu gcuguccgac gagaccaaga aguucgacaa gcagaagcag      660

agcgccaagc ucauccuguc cgagacgggg gacaaggcgc uggacaagga gaacaccaag      720

guggccaccc agaucagcaa ggccauccuc ggcuacaaga cccagcugga cgugcugcuc      780

aaguacgacg ccaucaacuc cagcgccugg aagaucgagc uggacgacga gaacaucgac      840

gacacgcugc ccgggcuccu gucccagcug gacgagaacc gccaggagau ggucagcauc      900

auccagaacc ucuacgccca ggugacccug aaccagaucg ugccgaacgg caugucccug      960

agcgagagca ugaucgagaa guacaacgcg caccgggacc accucaagcu gcugaagcag     1020

cucgaggaca agaucgacga ccccaagaag aagaagggcc uggaggccgc cuacgacgcc     1080

uacgucggga agaacaugaa gguggcgaac caggacgugu ucuacgccuc cgucaagaag     1140

aaccuggacg acagcgaccu cgccaagcag aucgccgacc ugaucgaggc ccagaaguuc     1200

augcccaagc agcgcaccuc ccagaacggc gugauccccc accagcugca ccagcucgag     1260

cuggaccaga ucaucgagaa ccagagcaag uacuacccgu ggcugaccgc ggccaacccc     1320

aaccccaagc gcauccacca ggccaaguac aagcucgacg agcugguggc cuuccggauc     1380

cccuacuacg ucgggccgcu ggugacgccc gaggagcagg agaaguccgc gaaccaggug     1440

uucgccugga ugaagcgcaa gcagcccggc accaucaccc ccuggaacuu cgaccagaag     1500

gucgaccgga uggccagcgc caaccgcuuc auccggcgca ugaccacgaa ggacaccuac     1560

cucaucggcg aggacgugcu gccggacgcc ucccugaucu acgagcgguu caaggugcug     1620

aacgagcuca accuggucaa ggcgaacuac caccgccugg cccccgccca gaagcaggcc     1680

gccuaccggg accucuucga gaaccagaag uccaucucca ccaagaagcu gcagaacuac     1740

cugguggacc agggguacuu caccgcgaag cccaagauca cgggccucag cgaccccgag     1800

cgcuucaacu ccagccuggg gaccuacauc gacuucaagg agaucuucgg cgaccagguc     1860

gacaacccgg accggcagga cgacuacgag aagaucaucg agugguccac cguguucgag     1920

gaccgccaga ucuacgaggc caagcugcgg acccucgacu ggcugacgga ggaccagauc     1980

caggcccuga agaccaagcg cuacaacggg uggggccggc ucagcaagaa gcugcugacc     2040

ggcaucgugg acgagaacgg ggagcgcauc aucgacgucc ucuggaacac caaccagaac     2100

uucaugcagg uccaguccca ggagagcuuc gccaagcgga uccacgaggc caacgcgaag     2160

cagaugaagg ugacgggcgu ggaggacauc cuggccgacg ccuacaccag cccccagaac     2220

aagaaggcca uccgccaggu gauccgggug gucgacgaca uccagaaggc gaugggcggg     2280

guggccccca ccagcguguc caucgaguuc acccgcagcg aggaccagaa cccccgccgg     2340

acccagagcc gcgaguccca gcugcagaac acguucaagg ucgacgccaa ggagaucgug     2400

gacagcgacc ucgugaccga gcuggagacc gucuccaaga gcaagaaggg gcugaccgac     2460

cggcucuacc uguacuucac gcagcugggc aaggacaugu acaccggcga cccgcucaac     2520

aucgacgagc uguccaccua cgacaucgac cacauccugc cccaggccuu caucaaggac     2580

gacagccucg acaaccgcgu gcuggugucc aaggccguca acaacgggaa guccaacaac     2640

gugcccguga agcuguucgg cgggaagaug ggcggguucu ggaaggagcu cgcggaccac     2700

cggcugauca gcaagcgcaa gcugaagcac cuccugaccg acccggacac gaucgacaag     2760

uacaccaugc ggggcuucau ccgccggcag cuggucgaga ccagccaggu gaucaagcuc     2820

guggcccaga uccugggcga caaguaccag aacgacgaca ccaagaucau cgaggucacg     2880

gccaagauga accaccagau gcgcgagaac uucaagcuga ucaagaaccg ggaggugaac     2940

gacuaccacc acgccgugga cgccuaccuc accgcguucg ucgggcagua ccuguacaag     3000

cgcuacccca agcugcggcc cuacuucgug uacggcaacu ucaagaaguu caacgaggac     3060

cacguguccu uccgcuacuu caacuuccuc cacgagcuga uggacgagaa caacaagcgg     3120

aucgucgagc ccgagaccgg ggagguggug cuggagcgcg agccggccau cgccuacaug     3180

aagcgggucu accacuucaa guucaugcuc aucagccagg agguguacac ccgccgggac     3240

gccauguuca accagacgau cuaccccgcc aaggaggcga accgccggaa gcugaucgcc     3300

aucaagcacg gcaagcccgu ggacgucuac ggcggguacu ccggcagcgu ggacgccuac     3360

auggccaucg ugcgcaucca cgacaagaag ggggacaagu acaaggucgu gggcgugccc     3420

augcgcgcgc uggagauccu caacgccacc cacgacaaga aggacuacga cgccaagcug     3480

aagcagaucc uggucccgaa guucaccaag gugaagaagu cccggaagac cggggagauc     3540

acgaagaagg uggaggacuu cgagaucguc cucggcaagg ugauguaccg ccagcuggug     3600

aucgacggcg acaagaaguu cauggucggg agcuccaccu accuguacaa cgcccggcag     3660

cucgugcuga gcgacagcuc ccuggacacc cucgccagca agggcacccu ggacuccgac     3720

gcggacagca agcgccuggu ggccgucuac gacgagaucg uggagaaggu cgacaaguac     3780

uucccccucu acgacaagaa cgccuuccgg uccgggcugc acaagggccg cgacaaguac     3840

cugcuccugc ccaaccggag cacguucaac ggggccaaga aggugcacuc cggcaagcgc     3900

gagauccuga acgagauccu caacggccug cacgccaacg cgacccucgg gggccugaag     3960

gaccugaaga ucagcacccc cuucgggaag augcagggcc aguccgggau cccgcugagc     4020

uccaacacca ucaucugcua ccagagcccc acgggccucu ucgagcaccg ggugucccug     4080

aaggaccugu ga                                                         4092


<210>  1917
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1917
auggagaaca accgcuacaa cgugggccug gacaucggga ccagcuccau cggcuucgcc       60

gucaccgacg agcaggacga gcucguccag gugaagggga agaacuacau cggcgugcgg      120

cuguucaagg aggggcagac ggccagcgag cgccgguccu uccgcaccac ccggcgccgg      180

cugaagcgcc ggaaguggcg ccucggccug cuggaggaga ucuucgcgcc cuaccucgcc      240

cagggcgacc cguacuuccu ggcccggaug aaggagagca acaucagccc ccgcgacgag      300

cggaagaagu acuacgggag ccugcuguuc uccgacgaca ccguguucua cgacaaguac      360

cccacggugu accaccuccg ccacgcccug augaccgagg accggaaguu cgacauccgc      420

gagaucuacc ucgccgucca ccacaucauc aaguaccggg gcaacuuccu gaucaacacc      480

cugaucaacg aguucagcuc caaggcggug gacuucaaga aggacgccga cauccucaac      540

gaccuguucg agcaguucua caucagcgac aucaacgacu ccuuccagcu gaacaccgcc      600

gacaugaacg agaucagcga gcuccugcug gacaacucca agacgcgccu cgaccggcag      660

cgcgagagcc ugaagaagcu ggccaagaag guggacgaca ucuccgucgc gaagaagcac      720

cagaagaucg ccaccgaguu cagcaaggcc auccucggga acaaggccaa guucgacgug      780

auccugaacg ugaccaccga cuccagcaac gacugguccu ucaagcugga ggacgaggac      840

gccgacgaga agaucgucgu gcuccuggag gagcugaaca cgccccagca ggaggcgcuc      900

aacaucaucc accaguggua cagccagauc agccugaacg gcaucgugcc ggacgggaag      960

uccaucagcg aggccaugau cuccagcuac gagcugcaca aggagcaccu cgcccugcug     1020

aagcagcaga ucaacaacac caaggacaag aagaaggccc acggccucca cgaggccuac     1080

aacgaguacg uccacggcaa caacaacaag aagcugaacc aggcggacuu cuacuccaag     1140

gugaagcaga accuggagag cucccucauc accgacgaga uccuggagcu gaucaacgcc     1200

gagaacuuca ugcccaagca gcggaccagc gccaacgggg ugauccccca ccagcuccac     1260

cagcaggagc uggaccgcau cauccagaac caggccaagu acuaccccuu ccuggccgag     1320

acgaacccga acgagaagcg cuccaagguc gcgaaguaca agcucgacga gcugguggcc     1380

uuccgggugc ccuacuacgu cggcccccug aucaccgcca ccgaccagaa gaagaccagc     1440

gggaaggagu ucgccuggau ggugcgcaag cagcccggcc ggaucacgcc guggaacuuc     1500

gacgagaagg ugaacaagau ggaguccgcc aacaccuuca uccgccggau gaccaccaag     1560

gacagcuacc ucaucgcgga ggacguccug cccgacgagu cccugcugua ccagaaguuc     1620

aaggugcuca acgagcugaa caaccugcgc gccgacggcc ggaagcucaa ggucagcgug     1680

aagcagaagg uguucaagga gcuguuccag cagcagaaga cgaucaccau cgucaagcug     1740

cagaacuacc ucaaggccga gugcuccuuc cugaccaccc ccgagaucag cgggcugucc     1800

aagcccgagc acuucgugag cucccucgcc acguacaucg accugaagaa gaucuucggc     1860

gaggacaucg acaacccggc gcgccagaac gacuucgagc ggaugaucga gugggccacc     1920

aucuucgagg acaagaacau cuacagcgag aagcugaacg agaucaagug gcucaacgac     1980

cgccagaaga aggagcugaa gaacaagaac uaccacgggu ggggcaagcu gagcgccaag     2040

cuccugaagg ggcugcggga cuccgacgag ugcagcauca ucgagcacau guggaacacc     2100

cagcagaacu ucaugcaggu ccaggccgag gaggacuucg cccagcgcau ccaggaggag     2160

aacaaccagc accucaaggg caacacccug gaggaggugc uggcggacgc cuacacgagc     2220

ccccagaaca agaaggccau ccgccaggug gugaagguca ucgaggacau ccagaaggcc     2280

augggcggga ucgcgcccca gaagaucucc aucgaguuca cccgggcccc cgaggagaag     2340

ccgcggcgcu ccguggagcg gcagcagcag aucgagaaga ucuacaagag cguggccaac     2400

gaggucgaga acgccggcgu gaaggacgag cuccagcgcu ucuccgaccg gaagcagacc     2460

cugaccgaca aguacuuccu guacuucacg cagcucggga aggacaugua caccggcaag     2520

agcaucaaca ucgaccagau cgggaacuac cagaucgacc acauccugcc ccaggccuuc     2580

aucaaggacg acucccugga caaccgcgug cucgucagcg ccgcggugaa caacggcaag     2640

uccgacaacg ugcccaccaa gaacgaccgc uacaaggucc gggagcacgg ccagcgccug     2700

uacgaccagg ggcugaucag caagcggaag uucaacaacc ucaucaccga ccccgacucc     2760

aucaacaagu ucaccgcccg cggcuucgug aagcggcagc ugguggagac gagccagguc     2820

auccgccugg uggccgaguu ccucuacaac aaguaccagu ccaagagcga gaucaucgag     2880

gugcgggcca agaugaacuc ccagcugcgc aaggacuucg ggcugaucaa gaaccggaac     2940

gucaacgacu accaccacgc gcucgacgcc uaccugacca ccuucaucgg caacuaccug     3000

uaccgccggu acccgaagcu ccgcgccuac uucaccuacg ggugcuucaa gaagguggac     3060

gacagccagc ugaaguacuu caacuuccug cacgacaucg aggacgacaa gaagaagauc     3120

aucaaggaca acgacacggg cgagauccuc uggcaccggg acgagggcau cgcccgcuuc     3180

aagcggaucu acaacuucaa guacaugcug gugacccacg agguccugac ccgccagggg     3240

gccauguuca agcagaccgu guacagcgcg gccguggccg gcuccaagaa gcucaucccc     3300

gucaaggagg accggcccau cgacaucuac gggggcuaca cgaacaacaa cgacgccuuc     3360

cuggccaucg ugaaggugac caagaagaag caguacaugg ucgugggcau cccccagcgc     3420

gcggugguca agcugaacga cgccgccaag caggggagca aggccuacca gaaggugcuc     3480

uacaacguga ucaagccgca guucaucaag aagaagaagg accggaagac cggcaagauc     3540

acccagaagc uggagaacuu cgagaucguc guguccaaga cgcuguaccg ccagcucauc     3600

aucgacgggg accagaagua caugcugggc agcuccgccu acaaguacaa cgcgaagcag     3660

cuggugcuca gcgaguacgc ccuggagguc cuggaccggu ccaucuucga gaagaaggag     3720

uacaccaccg uggaccucga caacguguac gacgagaucc uggcccaggu cgacaaguac     3780

uucagccugu acgacaagaa caaguuccgc cagaagcucc acgagucccg caagaucuuc     3840

accgagcugc ccaucaacaa ccuguucagc gggacgaagc uccugaaguc cggcaagcgg     3900

gagacccuga acgagauccu cgagggccug cacgccaacg cgaccaagaa ggaccugaag     3960

ucccucaagu ucuccacgcc ccuggggaug auccaguacc cgagcggcau cauccugucc     4020

gagaacgcca uccucugcua ccagagcccc accgggaucu ucgagcgccg ggugacccug     4080

aaggaccugu ga                                                         4092


<210>  1918
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1918
augaccaagc ugggcaagcc cuacgggauc ggcguggaca ucgggagcaa cuccaucggc       60

uucgccgccg ucgacgagaa cagccaccuc auccgccuga aggggaagac cgugaucggc      120

gcgcggcugu ucgaggaggg caaggccgcc gccgaccgcc gggccucccg cacgacccgg      180

cgccggcuca gccgcaaccg guggcgccug uccuuccugc gggacuucuu cgagagccac      240

aucaccccga ccgaccccaa cuucuucaug cgccagaagu acuccgagau cagccccaag      300

gacaaguccc gguacaagua cgagaagcgc cucuucaacg accggacgga cgcggaguuc      360

uacaaguacg agaacugccc caccauguac caccuccgca accggcugau gaccgacccg      420

agcaaggccg acgugcgcga gaucuacuuc gccauccacc acauccugaa gucccggggg      480

cacuuccuga cccccggcga cgccaaggac uucaacacga acaaggucgc ggugaacaag      540

aucuuccccg cccugcagga cgccuacgcc cagguguacc ccgaccucga caucaccuuc      600

gacgagaaca aggucaacga guucaagacc gugcugcuga acgagaaggc caccccgagc      660

gacacgcagc gcgcgcucgu gaaccugcug cucgccaagg agggggaccg ggacauccug      720

aagcagcaga agcaggcccu gaccgaguuc gccaaggccg ucgugggccu caagaccaag      780

cugaacgugg cgcuggggac cgaggucgac uccagcgagg ccacggccug gaacuucucc      840

cucggccagc uggacgacaa guggaccggc aucgagagcg ccaugaccga cgaggggacc      900

gagauccugg accagauccg cgaccucuac cgcgcccggc ugcugaacgg caucgugccc      960

gcggggaaga cgcucuccca ggccaaggug gacgacuacg cccagcacca cgaggaccug     1020

gagcuguuca aggacuaccu caagcagcug gaggacgacg gcaccgccaa ggccauccgc     1080

cagcuguacg accgguacau cgacggcgac gacgcgaagc ccuucguccg cgaggacuuc     1140

gugaaggccc ucaccaagga ggugaccgcc caccccaacg ccaagagccc ggagcugcug     1200

gagcggcucg cgcagcccga cuucaugcug aagcagcgca acaaggccaa cggggccauc     1260

cccguccaga ugcagcagcg ggagcuggac cagaucauca agaaccaguc cguguacuac     1320

gacuggcucg ccgcccccaa cccgguggag aagcaccgca agagcaugcc cuaccagcug     1380

gacgagcugc ucaacuuccg gauccccuac uacgucggcc cccuggugac ggcgaaggag     1440

cagaaggccg cccagggcgg gguguucgcc uggauggucc gcaaggaccc ggacgggaac     1500

aucacccccu acaacuucga cgagaaggug gaccgggagg ccagcgcgaa caccuucauc     1560

cagcgcauga agaccacgga caccuaccug aucggcgagg acguccuccc caagcagucc     1620

cugcuguacc agcgguacga ggugcugaac gagcucaaca acgugcgcgu caacaacgag     1680

aagcugagca ucgagcagaa gcagcagguc auccgggagc uguucgagcg ccacaacucc     1740

gugaccauca agcaguucgc cgagaaccuc cgggcccacg gcgacuacgc ccacaugccc     1800

gagauccgcg ggcuggccga cgagaagcgg uuccugagcu cccucagcac cuaccgccag     1860

cugaaguccc ugcucccgga ggcgaucgac gaccccgcca agcaggccga caucgagaac     1920

aucaucgccu ggagcacggu guucgaggac gcggccaucu ucaagaccaa gcugaaggag     1980

aucaacuggc uggacuccca gaccaucacc aagcucagca acauccgcua ccggggcugg     2040

gggcaguucu cccacaaguu ccugaacggc cugacgcucg ggaacggcca caccaucauc     2100

caggagcugu uccugagcaa caacaaccuc augcagaucc ugaccgacga gacccugcag     2160

aagaagauga ccgagcucaa cgccgacaag cugaaggccg ccaacaucaa cgacgcgauc     2220

gacgacgugu acacgucccc cagcaacaag aaggcccugc gccaggugcu ccgggucauc     2280

gaggacauca agcgcgccgc cgacggccag gaccccuccu ggcuguacgu ggagaccgcc     2340

gacgggggcg ggaccccggg caagcggacc cgcgcgcggc agcaccagcu gcaggagauc     2400

uacgccaacg ccgcccacga gcucaucgac agcgccgugc gcggggagcu ggaggacaag     2460

aucgcggaca aggccgacuu caacgaccgg cugguccucu acuucaugca gggcggccgc     2520

gacaucuaca cggggacgcc ccugaacauc gaccagcugu ccagcuacga caucgaccac     2580

auccuccccc agucccugau caaggacaac agccuggaca accgggugcu cgugcacgcc     2640

aucaucaacc gcgagaagaa cgccaccuuc gcgagcacca ucuacgccca gaagaugaac     2700

gccaccuggc ggcaguggca cgaggccggc cugaucuccg ggcgcaagcu gcggaaccuc     2760

cagaugcgcc cggaccagau cgacaaguac gccagcggcu ucgucgcgcg gcagcuggug     2820

gagacccgcc agaucaucaa gcugaccgag cagaucgugg ccgcccagua ccccgagacc     2880

aagaucaucg ccgucaaggc cggccucucc caccagcugc gggaggagcu ggaguucccc     2940

aagaaccgcg acgugaacca cuaccaccac gcguucgacg ccuuccucgc cgcccggauc     3000

gggacguacc ugcugaagcg cuuccccaac cuccaggccu ucuucaccua cggcaaguuc     3060

aagaaggcgg acgugaagaa gcugcgcggg uucaacuuca uccgggacau cacccacgcc     3120

gaggacaaga ucgucgugaa ggacaccggc gaggugaucu gggacaagaa gcgcgacguc     3180

gacgagcugg accggaucua caacuucaag cgcaugcuca ucacgcacga ggugcacuuc     3240

gagaaggccg accuguucaa gcagaccgug uacggggcca aggacagcaa ggaggccggc     3300

ggcaagaagc agcugaucuc caagaagaag ggguacccgg ucgacaucua cggcggguac     3360

acccaggaga ccggcagcua ccucuccgug gugcggcuga cgaagaaggc gauguacgcc     3420

gucgugaagg ugagcacccg cgacgccgcc aagcuggcgg ucgccaaguc caucagcgag     3480

cagaaggaga acgagacccu caacgacauc aucgacgaga agcuguccaa gaucagcaag     3540

aacgggaaga ucacccacga gcuguucgag guggugcucc cccgggucgg ccagaagacg     3600

cuguucaaga acuccaagua caaccuguuc cucgugaaca gcgacaccua caugcacaac     3660

uaccaggagc uguggaugcc ccgcgaguac cagaagaugu ggaaggacau cucccugagc     3720

aaguacggcg acgcccagac cgaggaccag cucgaccaga ucuucggguu caucgugucc     3780

caggucaaca gcuacuucaa ccuguacgac aucaaccagu uccggaagaa gcugaacgac     3840

gccgccgaca aguucgcgac ccuccccaug cgcgacacgg acgacaugca gggcaagauc     3900

gccaccaucg ggcagcugcu gaucggccuc caggccaacg ccgcccggag cgaccugcgc     3960

aaccuggaca ucaagacccc gcucgggcug cugcagaucc ccaacggcau cucccucgac     4020

agcgacaccu ccguggugua ccagagcccc acgggccugc uggagcggga gguccaccuc     4080

uccgaccugu ga                                                         4092


<210>  1919
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1919
augaccaagc ugaaccagcc cuacggcauc gggcucgaca ucggcagcaa cuccaucggg       60

uucgccgugg ucgacgccaa cagccaccuc cugcgccuca agggcgagac cgcgaucggg      120

gcccggcugu uccgcgaggg ccaguccgcc gccgaccggc gcgagagccg gacgacccgc      180

cggcgccugu cccggacccg cuggcggcuc agcuuccugc gcgacuucuu cgccccgcac      240

aucaccaaga ucgaccccga cuucuuccug cggcagaagu acuccgagau cagccccaag      300

gacaaggacc gcuucaagua cgagaagcgg cuguucaacg accgcacgga cgcggaguuc      360

uacgaggccu accccuccau guaccaccuc cggcugcgcc ugaugaccga cgccagccag      420

gccgacccgc gggagaucuu ccuggcgauc caccacaucc ugaagucccg cggccacuuc      480

cucacccccg gggccgccaa ggacuucaac accgacgagg uggaccugaa gcacaucuuc      540

cccgcccugg ccgaggcgua cgcccaggug uaccccgacc ucgagcugaa cuucgacagc      600

gcccuggccg acgacuucaa ggccaagcuc cuggacgagg acgcgaagcc guccgacauc      660

cagaaggccc uggucaaccu ccugcugagc uccgacggcg agaaggagau cgugaagaag      720

cggaagcagg uccucacgga guucgccaag gccaucaccg ggcugaagac caaguucaac      780

cuggcccucg gcaccgaggu ggacgaggcg gacgccagca acuggcaguu cuccaugggg      840

cagcuggacg acaaguggag caacaucgag acguccauga ccgaccaggg caccgagauc      900

uucgagcaga uccaggagcu guaccgcgcc cgccuccuga acggcaucgu gcccgccggg      960

augagccuga gccaggccaa gguggcggac uacggccagc acaaggagga ccucgagcug     1020

uucaagaccu acaucaagaa gcugaacgac cacgagcucg ccaagacgau ccgggggcug     1080

uacgaccgcu acaucaacgg cgacgacgcc aagcccuucc ugcgggagga cuucgucaag     1140

gcccucacca aggaggugac cgcgcacccc aacgaggugu ccgagcagcu gcugaaccgc     1200

augggccagg ccaacuucau gcucaagcag cggaccaagg ccaacggggc caucccgguc     1260

cagcugcagc agcgcgagcu ggaccagauc aucgccaacc agagcaagua cuacgacugg     1320

cucgcgaagc ccaaccccgu ggagaagcac cgggccucca agcccuacca gcuggacgag     1380

cugcugacgu uccgcauccc guacuacgug ggcccccuca ucacccccaa gcagcaggcc     1440

gagagcgggg agaacgucuu cgccuggaug gugcggaaga accccuccgg caacaucacc     1500

ccguacaacu ucgacgagaa gguggaccgc gaggccagcg cgaacaccuu cauccagcgg     1560

augaagacga ccgacaccua ccugaucgac gaggacgucc ugcccaagca gucccuccug     1620

uaccagaagu acgaggugcu gaacgagcuc aacaacgugc gcaucaacaa cgaggcccug     1680

agcaccgacc agaagcagcg gcugauccgc gaggucuucg agcggcacuc cagcgugacg     1740

aucaagcagg ucgccgacaa ccucguggcc cacggggacu ucgcccagcg ccccgagauc     1800

cggggccugg cggacgagaa gcgcuuccug uccagccucu ccaccuacca ccagcugaag     1860

gagauccugc acggcgccau cgacgacccc accaagcucc acgacaucga gaacaucauc     1920

accuggagca cgguguucga ggacgccgcc aucuucaaga ccaagcuggc ggagaucgac     1980

uggcuggacc cggccaccau caagaagcuc uccaacaucc gguaccgcgg guggggccag     2040

uucagccgca agcugcugga cgggcucaag cugggcaacg ggcacaccgu cauccaggag     2100

cugaugcucu ccaaccacaa ccugaugcag auccuggccg acgagacgcu caaggagacc     2160

augaccgagc ugaaccagga caagcugaag accgacgaca ucgaggacgu gaucaacgac     2220

gccuacacca gccccuccaa caagaaggcc cuccgccagg ugcugcgggu cguggaggac     2280

aucaagcacg cggccaacgg ccaggacccc agcuggcugu acaucgagac ggccgacggc     2340

cccgggaccg ccggcaagcg gacccagagc cgccagaagc agauccagac cguguacgcc     2400

aacgcggccc aggagcucau cgacuccgcc guccgcgggg agcuggagga caagaucgcc     2460

gacaaggcca gcuucacgga ccggcuggug cucuacuuca ugcagggcgg gcgggacauc     2520

uacaccggcg cgccgcugaa caucgaccag cugucccacu acgacaucga ccacauccuc     2580

ccccagagcc ugaucaagga cgacucccug gacaaccgcg ugcucgucaa cgccaccauc     2640

aaccgggaga agaacaacgu guucgccagc acccuguucg ccggcaagau gaaggcgacg     2700

uggcgcaagu ggcacgaggc cgggcugauc uccggccgga agcuccgcaa ccugaugcug     2760

cggcccgacg agaucgacaa guucgccaag ggguucgugg cccgccagcu cgucgagacc     2820

cggcagauca ucaagcugac cgagcagauc gccgcggccc aguacccgaa caccaagauc     2880

aucgccguga aggccggccu gagccaccag cuccgcgagg agcuggacuu ccccaagaac     2940

cgggacguga accacuacca ccacgccuuc gacgcguucc uggccgcccg caucggcacg     3000

uaccuccuga agcgguaccc caagcugacc cccuucuuca ccuacgggga guucgccaag     3060

gucgacguga agaaguuccg cgaguucaac uucaucggcg cccucaccca cgcgaagaag     3120

aacaucaucg ccaaggacac gggggagauc gugugggaca aggagcggga cauccaggag     3180

cuggaccgca ucuacaacuu caagcgcaug cugaucaccc acgaggucca cuucgacaug     3240

gccgaccucu ucgaccagac caucuacgcc gccaaggacu ccaaggagcg gggcgggagc     3300

aagaaccuga uccagaagaa gcagggcuac cugaccggca ucuacggggg cuacuccacg     3360

gagcgcggga gcuacguguc caucauccgg accuucaaga agggcgcgcc gaucuacaac     3420

aucgugaaca ucagcaccgc cgacgucucc cuccugagcg acaagcagcg cguguccgag     3480

gccagcgccg acgugcugcu caaggagcug aucaagaaga agcuggcgaa gcgcaagagc     3540

ggggagaaca ccaaguccgg cgccuucgag aucguccucc cccggguggg caagaacacg     3600

cuguucaaga acaagaagua cgggcuguuc cucguggaga gcaccaccga guuccacaac     3660

uaccaggagc uguggcugcc ccggaacgac cagaugcuca ucaaguccau ccagagccac     3720

aagaucgagg aguccaaggc cgacuacgag cugacccgca uguaccggaa caucgccagc     3780

caggucgacc acuacuucgg ccuguacacg auccgcaagu ucuccgagaa gcucaccgcc     3840

agcaucaacg acuacggggc gcugcccgug uacaaguccg agaccaccga cggcaaguac     3900

gaggugcuga gcaaccucuu cgaggggcug ggcuccggca cgagcaccgg gaaccugacc     3960

gccgucaagc uccagccgaa cuucggccgg guguaccuga ccuccgggau cgugcuggag     4020

gacgacacga gcaucaucua ccaguccccc accggccucu ucgagcgcag cgucaagcug     4080

gccgaccugu ga                                                         4092


<210>  1920
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1920
augaccaagc ugaaccagcc cuacggcauc gggcucgaca ucggcagcaa cuccaucggg       60

uucgccgugg ucgacgccaa cagccaccuc cugcgccuca agggcgagac cgcgaucggg      120

gcccggcugu uccgcgaggg ccaguccgcc gccgaccggc gcggcagccg gacgacccgc      180

cggcgccugu cccggacccg cuggcggcuc agcuuccugc gcgacuucuu cgccccgcac      240

aucaccaaga ucgaccccga cuucuuccug cggcagaagu acuccgagau cagccccaag      300

gacaaggacc gcuucaagua cgagaagcgg cuguucaacg accgcacgga cgcggaguuc      360

uacgaggacu accccuccau guaccaccuc cggcugcacc ucaugaccca cacccacaag      420

gccgacccgc gcgagaucuu ccuggccauc caccacaucc ucaagagccg ggggcacuuc      480

cucacccccg gcgccgcgaa ggacuucaac acggacaagg uggaccugga ggacaucuuc      540

cccgcccuga ccgaggccua cgcccaggug uaccccgacc ucgagcugac cuucgaccug      600

gccaaggcgg acgacuucaa ggccaagcuc cuggacgagc aggccacccc guccgacacg      660

cagaaggccc uggucaaccu ccugcugagc uccgacgggg agaaggagau cgugaagaag      720

cgcaagcagg uccucaccga guucgccaag gcgaucaccg gccugaagac caaguucaac      780

cuggcccucg ggacggaggu ggacgaggcc gacgccagca acuggcaguu cuccaugggc      840

cagcuggacg acaaguggag caacaucgag accuccauga ccgaccaggg caccgagauc      900

uucgagcaga uccaggagcu guaccgggcc cgccuccuga acgggaucgu gcccgcgggc      960

augagccugu cccaggccaa gguggccgac uacgggcagc acaaggagga ccucgagcug     1020

uucaagacgu accugaagaa gcucaacgac cacgagcugg ccaagaccau ccgcggccug     1080

uacgaccggu acaucaacgg cgacgacgcc aagcccuucc uccgcgagga cuucgucaag     1140

gcgcugacca aggaggugac cgcccacccc aacgagguga gcgagcagcu gcucaaccgg     1200

auggggcagg ccaacuucau gcugaagcag cgcacgaagg ccaacggcgc gaucccgauc     1260

cagcugcagc agcgggagcu cgaccagauc aucgccaacc agagcaagua cuacgacugg     1320

cuggccgccc ccaaccccgu cgaggcccac cgcuggaaga ugcccuacca gcuggacgag     1380

cugcucaacu uccacauccc guacuacgug gggccccuga ucacccccaa gcagcaggcg     1440

gaguccggcg agaacguguu cgccuggaug guccggaagg accccagcgg gaacaucacc     1500

ccguacaacu ucgacgagaa gguggaccgc gaggccuccg ccaacaccuu cauccagcgg     1560

augaagacga ccgacaccua ccugaucggc gaggacgugc uccccaagca gagccugcug     1620

uaccagaagu acgagguccu caacgagcug aacaacgugc gcaucaacaa cgagugccug     1680

ggcaccgacc agaagcagcg gcucauccgc gagguguucg agcggcacuc cagcgucacg     1740

aucaagcagg ucgccgacaa ccugguggcg cacggggacu ucgcccgccg gcccgagauc     1800

cgcggccugg ccgacgagaa gcgguuccuc uccagccugu ccaccuacca ccagcugaag     1860

gagauccucc acgaggccau cgacgacccc accaagcugc uggacaucga gaacaucauc     1920

accuggagca cgguguucga ggaccacacc aucuucgaga ccaagcucgc cgagaucgag     1980

uggcuggacc cgaagaagau caacgagcug uccgggaucc gcuaccgggg cugggggcag     2040

uucagccgca agcuccugga cggccugaag cucggcaacg ggcacaccgu gauccaggag     2100

cugaugcugu ccaaccacaa ccucaugcag auccuggcgg acgagacccu gaaggagacg     2160

augaccgagc ucaaccagga caagcugaag accgacgaca ucgaggacgu caucaacgac     2220

gccuacacca gccccuccaa caagaaggcc cugcgccagg ugcuccgggu ggucgaggac     2280

aucaagcacg ccgcgaacgg ccaggacccc agcuggcugu ucaucgagac ggccgacggg     2340

accggcaccg ccgggaagcg cacccagucc cggcagaagc agauccagac gguguacgcc     2400

aacgccgcgc aggagcugau cgacagcgcc gugcgcggcg agcucgagga caagaucgcc     2460

gacaaggccu ccuucaccga ccggcugguc cuguacuuca ugcagggcgg gcgcgacauc     2520

uacaccggcg cgccccucaa caucgaccag cugagccacu acgacaucga ccacauccug     2580

ccgcagagcc ucaucaagga cgacucccug gacaaccggg ugcuggugaa cgccaccauc     2640

aaccgcgaga agaacaacgu cuucgccagc acgcuguucg ccgggaagau gaaggccacc     2700

uggcggaagu ggcacgaggc cggccugauc uccggccgca agcugcggaa ccucaugcug     2760

cgccccgacg agaucgacaa guucgcgaag ggguucgugg cccggcagcu gguggagacc     2820

cgccagauca ucaagcucac cgagcagauc gccgccgcgc aguaccccaa cacgaagauc     2880

aucgccguca aggccggccu gagccaccag cugcgggagg agcucgacuu cccgaagaac     2940

cgcgacguga accacuacca ccacgccuuc gacgccuucc uggcggcccg gaucgggacc     3000

uaccugcuca agcgcuaccc caagcuggcc cccuucuuca ccuacggcga guucgccaag     3060

guggacguca agaaguuccg ggaguucaac uucaucgggg cccugaccca cgcgaagaag     3120

aacaucaucg ccaaggacac gggcgagauc gugugggaca aggagcgcga cauccgggag     3180

cucgaccgca ucuacaacuu caagcgcaug cugaucaccc acgaggugua cuucgagacc     3240

gccgaccugu ucaagcagac caucuacgcc gccaaggacu ccaaggagcg gggcgggagc     3300

aagcagcuca uccccaagaa gcagggcuac ccgacgcagg ucuacggggg cuacacccag     3360

gaguccggga gcuacaacgc gcuggugcgc guggccgagg ccgacaccac cgccuaccag     3420

gucaucaaga ucuccgccca gaacgcgagc aagaucgccu ccgccaaccu caagagccgg     3480

gagaagggca agcagcuccu gaacgagauc guggugaagc agcuggccaa gcgccggaag     3540

aacuggaagc ccuccgcgaa cagcuucaag aucgucaucc cccgcuucgg cauggggacg     3600

cuguuccaga acgccaagua cggccuguuc auggugaacu ccgacaccua cuaccggaac     3660

uaccaggagc uguggcucag ccgcgagaac cagaagcugc ugaagaagcu guucuccauc     3720

aaguacgaga agacccagau gaaccacgac gcccuccagg uguacaaggc caucaucgac     3780

caggucgaga aguucuucaa gcuguacgac aucaaccagu uccgggccaa gcugagcgac     3840

gcgaucgagc gcuucgagaa gcugcccauc aacaccgacg ggaacaagau cggcaagacg     3900

gagacccugc ggcagauccu caucgggcug caggccaacg gcacccgcag caacgugaag     3960

aaccugggca ucaagaccga ccucgggcug cuccaggugg gcuccgggau caagcuggac     4020

aaggacacgc agaucgucua ccagagcccg uccggccugu ucaagcggcg caucccccug     4080

gccgaccucu ga                                                         4092


<210>  1921
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1921
augggcgacc gcaaguacaa ccuggggcuc gacaucggca ccagcuccau cggguucgcc       60

gccguggacg agaacaacca gcccauccgg gucaagggca agaccgcgau cggggugcgc      120

cuguucgagg agggcaagac ggccgccgac cggcgcggcu uccggaccac ccgccggcgc      180

cugagccggc gccgguggcg caucaaccuc cugaacgaga ucuucgacgc ccaccuggcc      240

gagguggacc cgaccuuccu cgcgcggcug aaggagucca accgcagcaa ccuggacccc      300

aagaaguccu uccaggggag ccuccuguuc cccgagcgga aggacuacca guucuacgag      360

gaguacccca cgaucuacca ccuccgcaag gcccugaugg agaaggaccg gaaguucgac      420

uuccgcgaga ucuaccuggc cguccaccac aucaucaagu accggggcaa cuuccugaac      480

gggaccccga ugcgcuccuu caagguggag aacaucgagc ucgacacccu guucgaccag      540

cugaaccagc ucuacgccga gaucgugccc gacaacgagc uggcguucga ccuggcccag      600

gucgccgacg ugaaggacgu gcucagcucc accacgaucu acaagaugga caagaagaag      660

cagcugguca agaugaugcu gcuccccgcc agcaacaagg cccugcaguc cgagaacaag      720

aagaucguga cccaguucgu gaacgcgauc cugaacuaca aguucaagcu cgacguccug      780

cuccaggugg agaccgacgc cgacuggagc cugaagcuga acgacgaggg cgccgacgac      840

aagcuggagg aguucaccgg ggaccucgac gagaaccggc uggagaucau cgaccugcuc      900

cagcgccugc acaacugguu cucccugaac gagaucacga aggacggcaa cagccucucc      960

gccgccaugg uggagaagua cgagaaccac caccaccacc ugggccugcu caagaagguc     1020

aucgagaacc accccgacgc gaagaaggcc aaggcccuga aggagaccua caccgccuac     1080

guggggaaga ccgacgacaa gacgcagaac caggacgacu ucuacaaggc cguggagaag     1140

aaccuggacg acagcccgga cgcgaaggag aucaagcgcc ucauccagcu ggaccaguuc     1200

augcccaagc agcggaccgg ccagaacggg gccauccccc accagcugca ccagcaggag     1260

cucgaccaga ucaucgagaa gcaguccaag uacuaccccu uccuggccga gccgaacccc     1320

aacgucaagc gccggaagga cgcccccuac aagcuggacg agcugaucgc cuucaagauc     1380

cccuacuacg ugggcccgcu cgugaccccc gaggagcagg cgcagaacgg cgagaacguc     1440

uucgccugga ugaagcgcaa ggccgccggg cccaucaccc ccuggaacuu cgacgagaag     1500

guggaccgga uggagagcgc gaaccgcuuc auccggcgca ugacgaccaa ggacaccuac     1560

cuguucggcg aggacgugcu gccggccgag uccaugaucu accagaaguu cgucgugcuc     1620

aacgagcuga acaaccugaa gaucaacggg cggcaccuca gccugaagga caagcaggac     1680

gucuacaacg accuguucaa gcagcagaag accgugagca ucaaggcccu ccagaacuac     1740

uacgugacga agaagaaggc cgccaccgcg cccaccgucg gcgggcuggc cgaccccaag     1800

aaguuccugu ccagccucuc caccuacauc gacuucaaga acauguucgg cgagcgcgug     1860

aacgaccccc aguuccagga ggaccuggag cagaucgugg aguggagcac gaucuucgag     1920

gaccggggca ucuucaaggc caagcugcag gcccucgggu ggcuguccga gaagcagauc     1980

cagcagcugg ucgccaagcg cuacaagggc ugggggcggc ucagcaagaa gcugcugacc     2040

ggccucaaga acgcggaggg guacuccauc cuggacgaga uguggcgcag caccggcaac     2100

uucaugcaga uccagucccg gccggaguuc gccgcccuga uccagcaggc caacgagaag     2160

caguucgagg gcaacgaccc cgacaacgug ugggagaaca ucgagaacau ccucggggac     2220

gccuacacca gcccccagaa caagaaggcg auccgccagg uggucaaggu ggugcaggac     2280

aucgagaagg ccgucggcaa cccgcccgag aagaucgcca ucgaguucac gcgggaggcc     2340

gcggccaacc cccagcgcac ccagucccgg cugcgcaccc uggagaagcu cuacgagagc     2400

gccgaggagg ugguggacgc cgggcugacc gccgagcugg cggaguucaa ggagaacaag     2460

cacguccucu ccgacaagua cuaccuguac uucacgcagc ugggccgcga cguguacacc     2520

ggggacacca ucagccucga caagcugaac gacuacgacg uggaccacau ccugccccag     2580

uccuucauca aggacgacag ccucgacaac cggguccuga ccauccgcgc cgugaacaac     2640

ggcaaguccg acaacgugcc ggccaagaug uucggcaaga agauggggag cuucuggcgg     2700

uaccugcucg acaacggcau gaucuccaag cgcaaguaca acaaccugau caccgacccc     2760

gacaacauca gcaaguacgc ccagaagggg uucaucaacc ggcagcuggu cgagacgagc     2820

caggugauca agcucaccgc caacauccug aacggcaucu acgacaagga caccgagauc     2880

aucgaggugc ccgcgaagau gaacucccag augcgcaaga uguucgaccu ggucaaggug     2940

cgggagguga acgacuacca ccacgccuuc gacgccuacc ucaccaucuu caucggcaac     3000

uaccuguaca agugcuaccc caagcugcag ccguacuucg ucuacgacaa cuucaagaag     3060

uucgggaaca aggaggacau cggccacaag cgcuucaacu uccucgggaa gaucgagcgg     3120

gagaagaagg ugguggcccc cgagacgggc gagauccugu ggagcaacgu cgcccccaac     3180

gagaccauca agcagaucaa gaagguguac gacuacaagu ucaugaucgu gucccgcgag     3240

aucaccaccc ggcgcgcgga gcuguucaac cagacggucu accccaagaa cuaccacggg     3300

aagcucaucc cgaucaagga ggaccggccc accgaccugu acggcggcua cagcgggaac     3360

accgacgccu accuggccau cguggcccuc gaggacaaga agaagggcaa guacuucaag     3420

guggucggga uccccacccg cguggcggcc aagcuggaga agcugaagca gcaggacucc     3480

cagcaguacc uccaggcccu gcacaaggug aucgcgcccc aguucaccaa gagcacgaag     3540

aagggcauca agaagaccga guucgagauc guccuggaca aggugcacua ccggcagcuc     3600

gugcaggacg ggccggucaa gaugaugcug ggcuccagca ccuacaagua caacgccaag     3660

cagcuggugc ucuccgagaa ggcccuccag gugaucgccg acgaccgcaa guucgacgag     3720

acgcagaagg acgacaaccu gaucgccguc uacgacgaga uccugagcau cgugaaccag     3780

uccuucgacc uguacgacau caacggcuuc cggaagaagc ugaacgacaa ccgcgaccag     3840

uucaucgacc uccccgccga gaccaaguac gaggggcgga agguggucgc ccacggcaag     3900

cgcgagauga uccuggagau ccugaagggg cuccacgcga acgccgccuu cggcaaccug     3960

aagcccaucg gguucagcac cgccuucggc cagcuccagg ugcccaacgg caucauccug     4020

uccaagaacg ccauccugau ccaccagagc ccguccgggc uguucgagcg gaagaucaag     4080

cucagcgacc uguga                                                      4095


<210>  1922
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1922
auggagaagc aggaguacua ccugggccuc gacaugggga ucagcuccgu gggcugggcc       60

gucaccaacg acaaguacga gcugcugcgc aagaagggga aggacaugug gggcauccgg      120

gaguucgagg aggccgacag cuccgcggag cgccggaccc accgcgugaa ccggcgccgg      180

cgccagcgcc agguggcccg gauggggcuc cugaaggagu acuucagcga cgccaucgag      240

aaggucgacu ccaacuucuu cauccggauc gagaacagca aguacuucuu cgaggacaag      300

gacggccgcc uguccagcaa gaacggcauc uucgacgacg ccgacuacaa ggacguggac      360

uacuacacgg aguaccccac caucuuccac cuccggaagg cccugcugga caucgaggac      420

accgggaagg aguacgacgu gcgccucguc uaccuggcgc uggugaacau guucaagcac      480

cggggccacu uccuccugaa caccgagggg gacgcccugu ccagcgagca ggucaacacg      540

accuaccagg agcuccugga gauccuggag aacaacuacg acaucucccu cgagagccgc      600

gacaucaagg acgugaucga caucaucucc agcuccgaca acggccggaa gaagaagcac      660

gagcagaucc uggagcuguu cggggugaag aaguccgaca agcgcgccaa cgagcuccug      720

aagugccugu gcggccucag cgugaacgcc aagcugaucu ucgacgagaa cuacgaggag      780

aaggucgacc ugugcuucca cgacuucagc uacaccgaca agaucccgga ccucauggag      840

aagcuguccg aggaggagua cuacguggug gagcagcuca agagcaucua cgacuacagc      900

auccucgcgg gcacccugaa gggguacgac uaccuguccu gcgcccgggu cgagcucuac      960

gagaagcacc gcgaggaccu gaagcugcuc aaggcccugu accggcgcua caagacgaac     1020

gaggaguaca acgacauguu caagagcgac aaggagggca ccuacuccgc cuacgugaac     1080

agcuucaacu ccagcgacuc cagcgugaag aucaagaagu accgcuggaa caugaagggg     1140

cggaccaucg acaaccugua cggcaccauc aagaaggccu ucaaggacaa cacggacgac     1200

aaggacgucc aguccaugau ggagagcauc gacaaggagc aguuccuccc caagcagcug     1260

accggcgcga acggggugau ccccaaccag gugcacaaga aggagaugga gcgcauccug     1320

aagaaggccc agaacuaccu ccccuuccug aaggagaagg acgaguccga gcugaccguc     1380

agcgagcgga uccuccgccu guucaccuuc cagaucccgu acuacguggg cccccugggg     1440

gagaacucca agacgggcug ggugguccgg cgcgagggcg ggcgggugcu ccccuggaac     1500

aucgacgaga agaucgacau gagcgccacc uccgaggccu ucaucggcaa ccugauccgc     1560

aagugcaccu accugagcga cgagaagguc augcccaagg ccucccugag cuacgagcgg     1620

uacugcgugc ucaacgagau caacaacauc ugcaucgacg gggagcgccu ggacaccgag     1680

cugaagcaga agaucuacac ggagugcuuc augaagggca agcgggugac ccgcaagcag     1740

aucugcaacu accucgucgc gcaggggugc aucaaguccg acgagcaggu caccggcauc     1800

gacaucaacg ugaacagcag ccuguccagc uacgggaaga uguucgccau cuucggcgag     1860

caccugaagg aggacgccau ccggaaggug gccgaggaga ucaucuacug gggcaccauc     1920

uucuccgaca gcaagaagau guuccgcaag caccucacga aguacgugga gcaggggauc     1980

cugggcgagu cccaggugaa gcggauccug ggguacaagu ucaaggacug gggccgcuuc     2040

agcaaggccc uccugacccu gcaggggauc gacaagucca ccggcgagcu ccugagccug     2100

gagaacgcga ugugggagua cucccucaac uucauggagc ugaucaacag cgacgacuuc     2160

accuucaagg acgagcuggc caaccggcgc acguccgcca ucaagagccu cuccgaguuc     2220

accuacgagg accuggagga caccuacuuc agcgucccgg ugaagcggau gaucuggcag     2280

accauccugg ugguccgcga gaucgagcag aucaugggcu acgcccccaa gaagguguuc     2340

guggagaugg cccgcuccga ggaggagaag ggggacaagg gccggaagga cagccgcggg     2400

acgcagcucc uggagcugua caagaacauc aagaacaccg agaaccacga cuggaagaag     2460

gagaucuccg aggcgaacga gagcggcaag cuccggagca agaagcugua ccuguacuac     2520

acccagaugg gccgcgacau guacaccggg gaggagauca acaucgacau gcucuacgac     2580

gacuccaugu acgacaucga ccacaucuac ccccggcacu acgucaagga cgacagcauc     2640

gugaacaacc uggugcuggu caaccgccgg gccaaccagg acaucaagaa ggaccucuac     2700

cccaucccgg acaagaucau cuccaacccc aaggugcagg agcuguggaa gaugcugcac     2760

aagcagaacc ucaucacgga cgagaaguac aagcgccuga ugagccgguc ccccuucacc     2820

gagaagcagc ugggcgacuu caucgcccgc cagcucgugg agacccggca ggggaccaag     2880

ggcaucgccg agcugcuggg gcagcuguuc aaggacaccg agaucgucua cgcgaaggcc     2940

acgaacguga gcgacuuccg ccgggacaac ggcuucguga agucccgccu ggucaacgac     3000

uuccaccacg cccaggacgc cuaccugaac aucguggugg gcaacgucua cuacaccaag     3060

uucaccaaga accccuggaa cuucaucaag aaggacuucg acaaggaccg gaacaagaac     3120

ggguacaacc ucgccaagau guucgaccgc gacguggugc ggggcgacga caccgcgugg     3180

aucgccagca agaaggacac gcaggggacc aucgagaccg uccgcaagau gaugcggaag     3240

aacaccccgc ugaugacgcg cauguccuuc gagcagcacg gcgggaucgc caacgccacc     3300

cuguacagcg ccaagaaggc gaagguggag aacuacaucc cccucaaguc cagcgacauc     3360

cggcuguccg acgugaagaa guacggcggc uucaccagcg ccaccgucgc cuacuacuuc     3420

aucgugcacc acaaggaggg gaagaaggag aacacgacca ucgaggcccu gccccucuac     3480

ugcaagcgca agguggagaa cgaggagaac ggccugcuga aguacugcga ggagguccuc     3540

ggguacggcg acgugaagau ccuguucaac aagcugaaga uccagucccu ccuggacauc     3600

aacggguacc aggcccaccu gagcggcaag accggcaacc agcucacccu gcgcaacgcg     3660

augaaccugu gcgugcccga gaaguggacg gccuacauca agaagcucga gaagcugguc     3720

guggggcacg ugaaggagca ggagaucucc agcgaggcca acaucgagcu guacgaccuc     3780

cugaccgaca agcaccugaa cuccgucuac gccaagcggc cgaacgcgua cggccagaag     3840

cucaucgaca accgcagccg guucgugaac cugaagcccg agagccagug cgaggccauc     3900

cugcagaucc ucaagcugac caagaucggg aacaacgaga ccaaccugaa gcucaucggc     3960

gaguccgagc acagcgggaa gaugcugauc uccaagaacc ugaaggacac ggacaagccc     4020

gccaucaucu gccagagcgu gaccggccuc uacgagcgca agaucuacau cuucgacgag     4080

cagcuggaca aguga                                                      4095


<210>  1923
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1923
augaacaagc gcuacaacau cggccuggac aucgggacca acagcaucgg cugguccgug       60

gucaacgacc agagccagcu cgugcggauc aaggggaagc ugggcuacgg ggugcgccug      120

uacgacgagg gccagaccgc cgaggaccgg cgccucuucc ggacgacccg ccggcgccug      180

aagcggcgca aguggcggcu gggccuccug cgcgagaucu ucgagcccua caucaccccg      240

caggacgcca ccuucuuccu gcggcagaag caguccaacc ucagccccaa ggaccugcag      300

aagcagcagc cccagaucgg gcuguucaac gaccgccucg acuccgacuu ccacuaccag      360

uaccccacga ucuaccaccu ccgguacgcg cugaugaccg agaagcgcca guucgaccuc      420

cgggagaucu accuggccau gcaccacauc gucaaguacc gcggccacuu ccugaacgcc      480

gccgccgcga gcuccuucaa gagcggggag aucgaccucg ccaaggacuu cgccgcccug      540

aaccagaucu ucggcaccau cuucuccgag gugggguuca gccuguccga gagcaaccuc      600

gacgagauca aguccauccu gcuggacaac aaccucagcg cguccgugcg gcagcgcaag      660

gcccugccgc ugaucuacca gaccgccgcc agcaaggacu ccgagaagca guacaaggcc      720

aucgcgacgg agcuccugaa ggccauccug ggccucaagg ccaaguucaa ccugaucacc      780

ggcaucgagg ucgaggacgu gaaggccugg acccugagcu ucaacgccga ggacuucgac      840

gcgaagcucu ccgaccugaa caucgccgac aacgacgacg ccgugcagau caucgagauc      900

cugcgggccc ucuacagcgg gguccugcug accggcaucg ugcccgaggg gcagagcauc      960

ucccaggcca ugaucgacaa guacgaggcg cacaaggccc accucaucau gcugcgcagc     1020

auccgcgagc agcuguccga caaggagaac aaggcccucg agaaggccua cgacgacuac     1080

aucgaccacc ccgaggacaa gcagcacggc agccgggagg acuucuacgc cgcgaucacg     1140

aaggugcuga agaaggacuc cgaggacacc aacaucaucg agauccugaa gcucaucgag     1200

cuggaccagu ucaugcccaa gcagcgcacc aaggacaacg gcgccauccc gcaccagcug     1260

caccagcagg agcucgacca gaucaucgag aaccagaagc aguacuaccc cuggcuggcc     1320

gacaucaacc ccaacgagca gcggcagaag auggcccccu acaagcugga cgagcugguc     1380

accuuccgcg ugccguacua cguggggccc augaucacgg cggaggagca guuccagacc     1440

agcaacaaga aguucgccug gaugauccgg aaggagcccg gcgccaucac ccccuggaac     1500

uucgaccaga aggucgaccg ccaggccucc gccaacgagu ucaucaagcg gaugaccacg     1560

accgacaccu accuccuggc ggaggacgug cugccgaagc agagccuccu guaccagcgc     1620

uucgaggugc ugaacgagcu caacgggauc aagaucgacg accagcccau caccgucgag     1680

cagaagcagg ccaucuucaa cgacaaguuc augcagcagg cccacgugac ggugaagaac     1740

auccaggccu accuggucuc cgagggccag uacgccagcc ggcccaacau caccgggcug     1800

uccgacggcg agaaguucaa cagcucccuc agcaccuacc acgaccugaa gaagaucuuc     1860

ggcgacauga ucgacgaccc caacaagcag accgacuucg agaagaugau cgaguggucc     1920

acgaucuucg aggacgggaa gaucuucgcg gacaagcugc gcgagaucaa cuggcucacc     1980

gacgagcagc ggcagcgccu gagcaacaag cgguaccgcg gcugggggca gcuguccacc     2040

aagcuccuga ccaagaucgu ggaccagaac ggccagcgga ucaucgacau ccuguggaac     2100

acgaacgaca acuucaugcg caucauccac agcgacgacu ucgaccaggc cgugacccag     2160

gccaaccaga ugcuccuggc cgacaacgac acccaggacg ucaucaacga gcuguacacc     2220

uccccgagca acaagaaggc ccuccggcag auccugcugg uggucgcgga cauccagaag     2280

gccaugaagg ggcaggcccc cgcccgcauc cucaucgagu ucgcgcggga gaagcaggcc     2340

aacccccgcc ugagcacgca gcgccggcgc cagguggagc aggucuacca ggagcugaac     2400

aacgaccagc ucaacaagga gcuggccgac ccccaguacg ccaaccuguc caaggaccgg     2460

cuguuccugu acuucaugca gggcggccgc gacauguaca ccggggagag ccugaacauc     2520

gaccggcucu ccgaguacga caucgaccac auccugccgc aggccuucau caaggacaac     2580

agccuggaca accgcgugcu cguguccgcg aagaugaacc ggagcaaguc cgaccacgug     2640

ccccuggacu ucaccgagga gcgcuucggc cagaagaugc ggagccagug ggagcagaug     2700

cugcgcgccg ggcuccugac ccggcgcaag uacaacaacc ugacccucaa ccccgacucc     2760

cugaacaagu acacgaugag cggcuucauc aaccggcagc uggucgagac ccgccaggug     2820

aucaagcucg ccaccaaccu gcugguggac caguacgccg acgaggacgu ccagcucgug     2880

accgugaagu ccgggcugac gcaccagaug cgggaggacu ucgacuuccc caagaaccgc     2940

aaccugaaca acuaccacca cgccuucgac gcguaccuca ccgccuucgu cggccuguac     3000

cugcucaaga aguacccgaa gcuggcccac uacuucgugu acggccagua ccagcagggg     3060

uucaaggcca acgaccagug gcggaacuuc aacuuccuga acggccuccg ccaggagcag     3120

caggucgacg ccgagaccgg ggagcugauc uggcagaagg accacgaccu ggcguacaug     3180

aacaagaucu acgacuucaa gaagauccuc gugacccggg aggugcacac gaaccacggc     3240

gcccuguaca accagacccu guacaaggcc aaggacgaca aggccagcgg ccagggcggg     3300

cgccagcuca ucgcggccaa ggagaaccgg cccaccgcca ucuacggggg cuacuccggg     3360

aagaccgugg ccuacaugug ccugguccgc aucacggaga agaagggcga cuucuaccgc     3420

aucugcggcg ugcagaccgc cuggguggac gagcuggagc acaucgagga cgcgaccauc     3480

aagcuccagc ggcugaccgc ccugcucacg ccccaguuca gcgccaccaa gaagcagaag     3540

gacgaggucg ugaccguggu caagccguuc gaggugcugc ugcccaagau ccucaucaac     3600

cagcgcuucu acgacggggg ccaggagcug acccuggccu ccgccacgua caagaacaac     3660

gcgcaggagc ucgugcugga caagaaggcc guccaggugc ugaacgggca gcuccccaag     3720

gccgagcugg aggcccaggc caccgugguc uacgaccaga uccuggagcg ggugcagaag     3780

uacuuccccc ucuacgacau gaacagcuuc cgcgagaagc uguucguggg ccgggacaag     3840

uucaacgagc ugccguggca ggaccagugg gagaaccaca agcucaucca ggucgggcag     3900

aagaccaucc uggaccgcau ccugaucggc cuccacgcga acgccgccau gggcgaccug     3960

aaggcccuga acauguccac ccccuucggg aagcuccaga aguccaacgg caucagccug     4020

agccccgaga cgcagaucau cuaccagagc cccaccgggc uguucgagcg gcgcguggcc     4080

cucaaggacc uguga                                                      4095


<210>  1924
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1924
augaagaacc ugaaggagua cuacaucggc cucgacaucg ggaccgccag cgugggcugg       60

gccgucaccg acgaguccua caacaucccc aaguucaacg ggaagaagau guggggcgug      120

cgccuguucg acgacgcgaa gacggccgag gagcggcgca cccagcgggg gagccgccgg      180

cgccugaacc ggcgcaagga gcggaucaac cuccugcagg accuguucgc caccgagauc      240

uccaaggugg acccgaacuu cuuccuccgc cuggacaaca gcgaccugua ccgggaggac      300

aaggacgaga agcucaaguc caaguacacc cuguucaacg acaaggacuu caaggaccgc      360

gacuaccaca agaaguaccc cacgauccac caccugauca uggaccucau cgaggacgag      420

ggcaagaagg acauccggcu gcuguaccuc gccugccacu accugcugaa gaaccgcggc      480

cacuucaucu ucgaggggca gaaguucgac accaagaaca gcuucgacaa guccaucaac      540

gaccucaaga uccaccuccg ggacgaguac aacaucgacc uggaguucaa caacgaggac      600

cugaucgaga ucaucaccga caccacgcug aacaagacca acaagaagaa ggagcugaag      660

aacaucgucg gcgacaccaa guuccucaag gccaucagcg cgaucaugau cggguccagc      720

cagaagcugg uggaccuguu cgaggacggc gaguucgagg agaccacggu gaaguccguc      780

gacuucagca ccaccgccuu cgacgacaag uacuccgagu acgaggaggc ccucggggac      840

accaucagcc ugcugaacau ccucaagucc aucuacgaca gcuccauccu ggagaaccug      900

cucaaggacg ccgacaagag caaggacggc aacaaguaca ucuccaaggc guucgugaag      960

aaguucaaca agcacggcaa ggaccugaag acgcugaagc gcaucaucaa gaaguaccuc     1020

cccagcgagu acgccaacau cuuccggaac aagagcauca acgacaacua cguggccuac     1080

accaagucca acaucaccag caacaagcgc accaaggccu ccaaguucac gaagcaggag     1140

gacuucuaca aguucaucaa gaagcaccug gacaccauca aggagaccaa gcugaacagc     1200

uccgagaacg aggaccucaa gcugaucgac gagaugcuga ccgacaucga guucaagacg     1260

uucaucccca agcucaagag cuccgacaac ggggucaucc cguaccagcu gaagcugaug     1320

gagcucaaga agauccugga caaccagagc aaguacuacg acuuccugaa cgaguccgac     1380

gaguacggca ccgugaagga caagguggag agcaucaugg aguuccggau ccccuacuac     1440

gucgggcccc ucaaccccga cuccaaguac gccuggauca agcgcgagaa caccaagauc     1500

accccgugga acuucaagga caucguggac cuggacagcu cccgggagga guucaucgac     1560

cgccugaucg gccgcugcac guaccugaag gaggagaagg ugcuccccaa ggcgagccug     1620

aucuacaacg aguucauggu ccugaacgag cucaacaacc ugaagcugaa cgaguuccuc     1680

aucaccgagg agaugaagaa ggccaucuuc gaggagcugu ucaagaccaa gaagaaggug     1740

acccugaagg ccguguccaa ccuccugaag aaggaguuca accugacggg cgacauccuc     1800

cugagcggga ccgacggcga cuucaagcag gggcugaacu ccuacaucga cuucaagaac     1860

aucaucggcg acaaggucga ccgggacgac uaccgcauca agaucgagga gaucaucaag     1920

cucaucgugc uguacgagga cgacaagacc uaccugaaga agaagaucaa gagcgccuac     1980

aagaacgacu ucaccgacga cgagaucaag aagaucgccg cgcucaacua caaggacugg     2040

gggcggcugu ccaagcgcuu ccugacgggc aucgagggcg uggacaagac caccggggag     2100

aagggcagca ucaucuacuu caugcgggag uacaaccuca accugaugga gcugaugagc     2160

gggcacuaca ccuucaccga ggaggucgag aagcucaacc ccguggagaa ccgcgagcug     2220

ugcuacgaga ugguggacga gcuguaccuc ucccccagcg ucaagcggau gcuguggcag     2280

ucccugcgcg uggucgacga gaucaagcgg aucaucggca aggacccgaa gaagaucuuc     2340

aucgagaugg cccgcgccaa ggaggccaag aacagccgga aggagucccg caagaacaag     2400

cuccuggagu ucuacaaguu cgggaagaag gccuucauca acgagaucgg cgaggagcgg     2460

uacaacuacc ugcucaacga gaucaacagc gaggaggagu ccaaguuccg cugggacaac     2520

cuguaccugu acuacacgca gcucggccgg ugcauguaca gccuggagcc caucgaccug     2580

gcggaccuca aguccaacaa caucuacgac caggaccaca ucuaccccaa gagcaagauc     2640

uacgacgacu cccuggagaa ccgcgugcug gugaagaaga accucaacca cgagaagggg     2700

aaccaguacc ccaucccgga gaagguccug aacaagaacg ccuacggcuu cuggaagauc     2760

cuguucgaca aggggcucau cggccagaag aaguacaccc ggcugacccg ccggaccccc     2820

uucgaggagc gcgagcuggc cgaguucauc gagcgccaga ucguggagac gcggcaggcc     2880

accaaggaga ccgccaaccu ccugaagaac aucugccagg acagcgagau cguguacucc     2940

aaggcggaga acgccagccg cuuccggcag gaguucgaca ucaucaagug ccgcaccguc     3000

aacgaccugc accacaugca cgacgccuac cucaacaucg ugguggggaa cgucuacaac     3060

acgaaguuca ccaagaaccc ccugaacuuc aucaaggaca aggacaacgu gcgguccuac     3120

aaccuggaga acauguucaa guacgacgug guccgcggca gcuacaccgc cuggaucgcg     3180

gacgacuccg agggcaacgu gaaggccgcc accaucaaga aggugaagcg ggagcucgag     3240

gggaagaacu accgcuucac gcggaugagc uacaucggca ccgggggccu guacgaccag     3300

aaccugaugc gcaagggcaa ggggcagauc ccccagaagg agaacaccaa caagagcaac     3360

aucgagaagu acggcgggua caacaaggcc uccagcgccu acuucgcgcu caucgagucc     3420

gacggcaagg ccgggcggga gcgcacccug gagacgaucc cgaucauggu cuacaaccag     3480

gagaaguacg gcaacaccga ggccguggac aaguaccuga aggacaaccu cgagcugcag     3540

gaccccaaga uccugaagga caagaucaag aucaacagcc ucaucaagcu ggacggcuuc     3600

cuguacaaca ucaaggggaa gaccggcgac ucccucagca ucgccggguc cgugcagcug     3660

aucgucaaca aggaggagca gaagcugauc aagaagaugg acaaguuccu cgugaagaag     3720

aaggacaaca aggacaucaa ggugaccagc uucgacaaca ucaaggagga ggagcugauc     3780

aagcuguaca agacgcucuc cgacaagcug aacaacggca ucuacagcaa caagcggaac     3840

aaccaggcca agaacaucuc cgaggcgcug gacaaguuca aggagaucag caucgaggag     3900

aagaucgacg uccucaacca gaucauccug cuguuccagu ccuacaacaa cgggugcaac     3960

cucaagagca ucggccuguc cgccaagacc ggcguggugu ucauccccaa gaagcugaac     4020

uacaaggagu gcaagcucau caaccagagc aucaccgggc uguucgagaa cgaggucgac     4080

cugcucaacc uguga                                                      4095


<210>  1925
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1925
auguacaaga aggacuacac cgugggccug gacaucggga gcaccuccgu cggcuucgcc       60

gccaucgacg acgacuacca gcccauccgc cucaagggga agcgggugau cggcgugcgc      120

cuguucgagg aggggaagac ggcggccgag cggcgcaacu uccggaccac ccgccggcgc      180

cugaaccggc gcaaguggcg gcucagccug cuggaggaga ucuucgaccc guacauggcc      240

aaggucgacc ccaccuucuu cgcccgccuc aagcagucca accuguuccc caaggacaag      300

aacaagaagu ucacgggcag ccuccucuuc cccaagacca ccgacuccaa guucuacgac      360

gaguacccga ccaucuacca ccuccgguac caccugauga acgacagccg caaguucgac      420

cuccgggaga ucuuccuggc cauccaccac aucgugaagu accgcggcaa cuuccugaac      480

uccgcgccca ucgccagcuu cuccacggac aagaucaacc ucguggacga cuucgagacc      540

gucaacgccg ccuaccugca gaucaacagc uccaaccccg ugcagcugga caccgaccag      600

gcggaccagg ugagccggau ccuccuggac aacaagcugu ccaaccucga caagcagaag      660

caggccgcca aggcccuggc ccagaagucc ggggacaaga agcaggacaa gaucaacaac      720

accacgauca agcagaucug caaggcgcug cucggcuaca aguucgaccu ggccgucgug      780

cugaacaucg acagcgacga ccacaacaag uggaagcucc agcugagcga cgagaacauc      840

gacucccgcc uggccgagcu caccggggag cugaacgagg cccagcugga cauccucaac      900

gugcugagcc ggcuguacuc ccagaucacc cucaacgaca ucguccccag cggcaugacc      960

cugagcgagg ccaugaucga gaaguacaag gcgcacaaga agcaccugaa gaugcucaag     1020

gacuucgccc gcucccugag caagaaggac uacggccagg ugauggaggc cuacgccgag     1080

uacaucggga acaucccgga guccgccagc uccaaggcgc agaagaagau cagccaggac     1140

gacuucuaca agaagaucaa gcgguaccug gacgacucca agcucggcca gaagaucgcc     1200

gagcugaucg ccaagaacga guucaugccc aagcagcgca cgaaccagaa cggggugauc     1260

cccuaccagc ugcaccucaa ggagcugaac cagaucaucg agaaccagag caaguacuac     1320

cccuggcugg ccgagcucaa cccgaacgag aagcgccggg gcaaggccaa guacaagcug     1380

uccgagcugg ucgcguuccg cauccccuac uacguggggc cccugaucac ccaggaggag     1440

cagcagaaga ccagcaacgc caacuucgcc uggaugaucc ggaaguccga gggccgcauc     1500

acccccugga acuucgacga gaagguggac cggaucgcca cggcgaaccg cuucaucaag     1560

cggaugacca ccaaggacac cuaccucauc ggcgaggacg uccugccggc ccacagccug     1620

cucuacgagc gcuucaaggu gcugaacgag cugaacauga uccgggugaa caaccaccgc     1680

cucuccgucg ggcagaagca ggacguguuc gagaagcugu uccagaagca caagacggug     1740

aagacccggg agcugaucaa gcagcucaug gccagcggcc ugcccaccca gccccgcauc     1800

accgggcugu ccgaccccgc caaguucaac acgagccucg gcaccuacaa caacuucgcc     1860

aagauccugg gggacgagau caaccagccg gaccggcagg aggacuucga gaagaucauc     1920

gaguggauca ccaucuucga cgacaaggac auccugaaga ccaagcucuc cgagcugagc     1980

uggcuggacg agaagcagau caacgcgcuc aucaacaaga acuacuccgg cuggggccgc     2040

cugagccgga agcugcucac ggagcugacc aacaagaagg ggcagagcau caucgaccgc     2100

auguggaaca cccagaaguc cuucaucgag aucaucaacg agcccgucuu cgccgagcag     2160

aucaaggacg ccaaccagga ccagcuggac gaggacgacu acgagaccgu gcuccacgac     2220

gccuacacga gcccccagaa caagaaggcc auccggcagg ucaucaaggu gguggacgac     2280

aucguccgcg cggccggccg ggcccccaag uucaucuccc uggaguucgc ccgcgaggac     2340

caggagagcc gccggaccca gucccgccug agccggauca agaagaucua cgagaccacc     2400

accaaggagc ucguggccaa cgaccagauc cgcgaggagc uggggaacgu gaaggaccug     2460

aacgaccggc uguuccugua cuucacgcag cugggccgcg acgcguacac cggggagcgg     2520

aucaacaucg acgacaucuc caccaaguac gacaucgacc acaucuuccc gcgcgccuuc     2580

aucaaggacg acagccucga caacaagguc cuggugaagc gggccgugaa caacggcaag     2640

uccaacaacg ucccccugaa gcuguucggc gccaagauga agcccaucug gaagaugugg     2700

aaggaccacc agcugaucag caagcgcaag uacgagcacc ugaugaccaa ccccgacgcg     2760

aucgacaagu acaaggccaa cggguucauc aaccggcagc ucaucgagac gcgccagauc     2820

aucaagcugg ccgccaacau ccuggccaac cgguacucca ccgaggacac caagaucauc     2880

gaggugaagg cgaaccucaa ccaccagcug cgccagagcc ugcaccucua caagaaccgc     2940

caggugaacg acuaccacca cgccgucgac ggcuaccugu ccgccuucgu ggggcaguac     3000

cuguaccacc gguacccgag ccuccagucc uacuucgugu acggccagua ccagcgguuc     3060

uucgacaagc agguccagga gaacaugaag uucaaccgcu ucaacuuccu guacgaccug     3120

accgaccggg acaacgacca gaucgugguc aagagcacgg gggagaucgu gggcgagaag     3180

uccaagcuca ucgagcagau caagugggug uaccgcuaca aguucaugcu ggugagccag     3240

gaggucuuca ccaagaccgg cccccuguuc gaccagacca ucuaccccgc cgggagcaac     3300

aagucccggc ucaucccgau caagaaggac cgccccacgg acaucuacgg cggguacagc     3360

uccagcaccg acgccuacau gucccugauc cgcgugcccg acaagaagga ggacaaguac     3420

aagguggucg gcgugcccag ccggucccug gcgagccuca accgcgccga gaagcggggc     3480

cacgacgccu accuggacca gcugcgccag gugaucgcca agucccagaa gaaggaguuc     3540

cagaucauca ucccgaagcu ccgguaccgc cagcugauca ucgacggggg ccugaaguac     3600

acccucggga gcuccgccua ccaguacaac gcgcggcagc ugguccugag cgagaagucc     3660

auggaggugc ucgugaccga cuucagcaag gucacgaccg accuggacaa gcagaaccag     3720

aagcugaucg ccguguacga cgacauccuc gacaagguga accgcugccu gccccuguac     3780

gacaagaacc aguuccggaa ggcccucaac ggcgcccgcg acaaguucgu ccagcugccc     3840

auccugccca aguucaacgg gaacaagaag gaggaggcgg gcaaggugga gauccucaac     3900

gagauccugc ugggccucca cgacgacgcc uccaccggga accugaagcc gcugggcauc     3960

aagacccccu ucgggaugcu ccagacgcag agcggcauca ccauguccag caacgccgug     4020

aucgucaacc agucccccac cgggcuguuc gagcaccgga ucuaccugaa ggcccuccug     4080

aacaagcuga acaaguga                                                   4098


<210>  1926
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1926
augaccaagc ugaaccagcc cuacggcauc gggcucgaca ucggcagcaa cuccaucggg       60

uucgccgugg ucgacgccaa cagccaccuc cugcgccuca agggcgagac cgcgaucggg      120

gcccggcugu uccgcgaggg ccaguccgcc gccgaccggc gcggcagccg gacgacccgc      180

cggcgccugu cccggacccg cuggcggcuc agcuuccugc gcgacuucuu cgccccgcac      240

aucaccaaga ucgaccccga cuucuuccug cggcagaagu acuccgagau cagccccaag      300

gacaaggacc gcuucaagua cgagaagcgg cuguucaacg accgcacgga cgcggaguuc      360

uacgaggacu accccuccau guaccaccuc cggcugcacc ugaugaccca cacccacaag      420

gccgacccgc gcgagaucuu ccuggccauc caccacaucc ucaagagccg ggggcacuuc      480

cugacccccg gcgccgcgaa ggacuucaac acggacaagg uggaccugga ggacaucuuc      540

cccgcccuga ccgaggccua cgcccaggug uaccccgacc ucgagcugac cuucgaccug      600

gccaaggcgg acgacuucaa ggccaagcuc cuggacgagc aggccacccc guccgacacg      660

cagaaggccc uggucaagcu ccugcugagc uccgacgggg agaaggagau cgugaagaag      720

cgcaagcagg uccucaccga guucgccaag gcgaucaccg gccugaagac caaguucaac      780

cuggcccucg ggacggaggu ggacgaggcc gacgccagca acuggcaguu cuccaugggc      840

cagcuggacg acaaguggag caacaucgag accuccauga ccgaccaggg caccgagauc      900

uucgagcaga uccaggagcu guaccgggcc cgccuccuga acgggaucgu gcccgcgggc      960

augagccugu cccaggccaa gguggccgac uacgggcagc acaaggagga ccucgagcug     1020

uucaagacgu accugaagaa gcucaacgac cacgagcugg ccaagaccau ccgcggccug     1080

uacgaccggu acaucaacgg cgacgacgcc aagcccuucc uccgcgagga cuucgucaag     1140

gcgcugacca aggaggugac cgcccacccc aacgagguga gcgagcagcu gcucaaccgg     1200

auggggcagg ccaacuucau gcugaagcag cgcacgaagg ccaacggcgc gaucccgguc     1260

cagcugcagc agcgggagcu cgaccagauc aucgccaacc agagcaagua cuacgacugg     1320

cuggccaagc ccaaccccgu ggagaagcac cgcgccucca agcccuacca gcuggacgag     1380

cugcucaccu uccggauccc guacuacgug gggccccuga ucacccccaa gcagcaggcc     1440

gagagcggcg agaacgucuu cgcguggaug gugcgcaaga accccuccgg gaacaucacc     1500

ccguacaacu ucgacgagaa gguggaccgg gaggccagcg ccaacacguu cauccagcgc     1560

augaagacca ccgacaccua ccugaucgac gaggacgucc uccccaagca gucccugcug     1620

uaccagaagu acgaggugcu caacgagcug aacaacgugc ggaucaacaa cgaggcccug     1680

agcacggacc agaagcagcg ccucauccac gaggucuucg agcggcacuc cagcgugacc     1740

aucaagcagg ucguggacaa ccugguggcc cacggcgacu ucgcgcagcg ccccgagauc     1800

cggggccugg ccgacgagaa gcgcuuccuc uccagccugu ccaccuacca ccagcugaag     1860

gagauccucc acggggccau cgacgacagc accaagcugc acgacaucga gaacaucauc     1920

acguggucca ccgucuucga ggacgccgcc aucuucaaga ccaagcuggc ggagaucgac     1980

uggcucgacc ccgccaccau caagaagcug agcaacaucc gguaccgcgg cugggggcag     2040

uucucccgga agcugcucga cggccugaag cuggggaacg gccacaccgu gauccaggag     2100

cucaugcuga gcaaccacaa ccugaugcag auccucgccg acgagacgcu gaaggagacc     2160

augaccgagc ugaaccagga caagcucaag accgacgaca ucgaggacgu gaucaacgac     2220

gccuacacgu ccccgagcaa caagaaggcg cugcgccagg uccugcgcgu gguggaggac     2280

aucaagcacg ccgccaacgg ccaggacccc uccuggcucu acaucgagac cgccgacggg     2340

accggcaccg ccgggaagcg gacgcagagc cgccagaagc agauccagac cgucuacgcg     2400

aacgccgccc aggagcugau cgacagcgcc gugcggggcg agcuggagga caagaucgcc     2460

gacaaggcgu ccuucaccga ccgccucgug cuguacuuca ugcagggcgg gcgggacauc     2520

uacaccggcg cgccccugaa caucgaccag cucagccacu acgacaucga ccacauccug     2580

ccccaguccc ugaucaagga cgacagccuc gacaaccgcg uccuggugaa cgccacgauc     2640

aaccgggaga agaacaacgu guucgccucc acccuguucg ccagcaagau ggccgccacc     2700

uggcgcaagu ggcacgaggc cgggcucauc uccggccgga agcugcgcaa ccugaugcuc     2760

cggccggacg agaccgacaa guucgcccac ggguucgucg cgcgccagcu gguggagacg     2820

cggcagauca ucaagcugac cgagcagauc gccgccgccc aguaccccaa caccaagauc     2880

aucgccguga aggcgggccu cagccaccag cugcgcgagg agcuggacuu ccccaagaac     2940

cgggacguca accacuacca ccacgccuuc gacgccuucc ucgccgcccg caucggcacc     3000

uaccugcuga agcgguaccc gaagcucgcg cccuucuuca cguacgggga guucgccaag     3060

guggacguga agaaguuccg cgaguucaac uucaucggcg cccugaccca cgccaagaag     3120

aacaucaucg ccaaggacac cggggagauc gucugggaca aggagcggga cauccaggag     3180

cuggaccgca ucuacaacuu caagcggaug cucaucaccc acgaggugca cuucgagacg     3240

gcggaccugu ucgaccagac caucuacgcc gccaaggacu ccaaggagcg cggcgggcgc     3300

augagccuga uccccaagaa gcucggcuac cugaccggca ucuacggggg cuaccagacc     3360

gagcgggggu ccuacgugag caucauccgc acguucaaga agggcgagac caucuacaag     3420

gucgugaaca uccugaccgc cgacgugucc cuccugagcg agaaggagaa ggucuccaag     3480

acccuggccg acaucuaccu caaggagcug aucgcgaaga agcugagcaa ggggaagucc     3540

ggcaagaaca ucgccagcga gcccuucgag aucgugcucc cgcggguggg caaggacagc     3600

cuguucaaga acaagaagua cgggcuguuc cucaucgagu ccgccacgga gcugcacaac     3660

uaccaggagc uguggcuccc ccgcaacgac cagcugcuga ucaagcggau ccaggcccgc     3720

cagaucgagg agagcaaggc ggacuacgag cucacccgga uguaccgcaa caucgccugg     3780

caggucgacc acuacuuccg gcuguacaag auccgcaagu ucgccgagaa gcugcaggcc     3840

uccguggagg acuucgccgg ccuccccgug uacaagaacg agaccagcga cgggaagguc     3900

gaggugcugg cguccgugcu gaagcggcuc aacggcgccg ccgacaaggg gcgcaccaac     3960

cccacgaccg ucaagcugca gagcgacuuc ggccggaccc ugcucaccaa cggcaucgug     4020

cuggaggacg acuccagcau cguguaccag uccccgaccg ggcuguucaa gcgcagcguc     4080

aagcucgugg accuguga                                                   4098


<210>  1927
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1927
augaccaagg aguacuaccu gggccucgac guggggacca acagcgucgg cugggccgug       60

acggacuccc aguacaaccu gugcaaguuc aagaagaagg acaugugggg gauccgccug      120

uucgagagcg ccaacaccgc gaaggaccgg cgccuccagc ggggcaaccg ccggcgccug      180

gagcggaaga agcagcgcau cgaccugcuc caggagaucu ucucccccga gaucugcaag      240

aucgacccga ccuucuucau ccggcugaac gagagccgcc ugcaccucga ggacaagucc      300

aacgacuuca aguacccccu guucaucgag aaggacuaca gcgacaucga guacuacaag      360

gaguucccca ccaucuucca ccuccggaag caccugaucg aguccgagga gaagcaggac      420

auccgccuga ucuaccuggc ccuccacaac aucaucaaga cgcgggggca cuuccugauc      480

gacggcgacc ugcagagcgc caagcagcuc cgccccaucc uggacaccuu ccugcucucc      540

cugcaggagg agcagaaccu gagcgugucc cucagcgaga accagaagga cgaguacgag      600

gagauccuga agaaccgguc caucgccaag agcgagaagg ucaagaagcu gaagaaccuc      660

uucgagaucu ccgacgagcu ggagaaggag gagaagaagg cccagagcgc ggugaucgag      720

aacuucugca aguucaucgu gggcaacaag ggggacgucu gcaaguuccu gcgcgugucc      780

aaggaggagc ucgagaucga cagcuucucc uucagcgagg gcaaguacga ggacgacauc      840

gugaagaacc uggaggagaa ggucccggag aagguguacc uguucgagca gaugaaggcc      900

auguacgacu ggaacauccu cguggacauc cuggagaccg aggaguacau cagcuucgcc      960

aaggucaagc aguacgagaa gcacaagacc aaccugcggc uccugcgcga caucauccug     1020

aaguacugca cgaaggacga guacaaccgg auguucaacg acgagaagga ggccgggucc     1080

uacaccgcgu acgugggcaa gcucaagaag aacaacaaga aguacuggau cgagaagaag     1140

cgcaaccccg aggaguucua caagagccug gggaagcugc ucgacaagau cgagccccug     1200

aaggaggacc uggaggugcu caccaugaug aucgaggagu gcaagaacca cacccugcug     1260

cccauccaga agaacaagga caacggcguc aucccgcacc aggugcacga gguggagcuc     1320

aagaagaucc uggagaacgc caagaaguac uacuccuucc ugacggagac cgacaaggac     1380

ggcuacagcg ucgugcagaa gaucgagucc aucuuccggu uccgcauccc cuacuacgug     1440

gggccccuga gcacccgcca ccaggagaag ggcuccaacg ucuggauggu gcggaagccc     1500

gggcgcgagg accggaucua cccguggaac auggaggaga ucaucgacuu cgagaagucc     1560

aacgagaacu ucaucacccg caugacgaac aagugcaccu accucaucgg cgaggacgug     1620

cugcccaagc acagccugcu cuacagcaag uacauggucc ugaacgagcu gaacaacgug     1680

aagguccggg gcaagaagcu ccccaccucc cugaagcaga agguguucga ggaccuguuc     1740

gagaacaaga gcaaggugac cgggaagaac cuccuggagu accugcagau ccaggacaag     1800

gacauccaga ucgacgaccu cuccggcuuc gacaaggacu ucaagacgag ccugaagucc     1860

uaccuggacu ucaagaagca gaucuucggg gaggagaucg agaaggagag cauccagaac     1920

augaucgagg acaucaucaa guggaucacc aucuacggca acgacaagga gaugcucaag     1980

cgcgucaucc gggccaacua cuccaaccag cugaccgagg agcagaugaa gaagaucacc     2040

ggguuccagu acagcggcug gggcaacuuc uccaagaugu uccugaaggg gaucagcggc     2100

agcgacgugu ccacggggga gaccuucgac aucaucaccg ccauguggga gaccgacaac     2160

aaccucaugc agauccugag caagaaguuc acguucaugg acaacgugga ggacuucaac     2220

uccggcaagg ucgggaagau cgacaagauc accuacgaca gcaccgugaa ggagauguuc     2280

cugucccccg agaacaagcg cgccgugugg cagaccaucc aggucgcgga ggagaucaag     2340

aaggugaugg gcugcgagcc gaagaagauc uucaucgaga uggcccgggg cggggagaag     2400

gugaagaagc gcaccaagag ccggaaggcc cagcuccugg agcuguacgc cgccugcgag     2460

gaggacugcc gcgagcucau caaggagauc gaggaccggg acgagcgcga cuucaacucc     2520

augaagcugu uccuguacua cacgcaguuc ggcaagugca uguacagcgg ggacgacauc     2580

gacaucaacg agcucauccg gggcaacucc aagugggacc gcgaccacau cuacccccag     2640

agcaagauca aggacgacuc caucgacaac cugguccugg ugaacaagac cuacaacgcg     2700

aagaagucca acgagcuccu gagcgaggac auccagaaga agaugcacag cuucuggcug     2760

ucccuccuga acaagaagcu gaucaccaag agcaaguacg accggcucac gcgcaagggg     2820

gacuucaccg acgaggagcu guccggcuuc aucgcccggc agcuggugga gacccgccag     2880

agcaccaagg ccaucgccga caucuucaag cagaucuaca gcuccgaggu cguguacguc     2940

aagagcuccc ucgugagcga cuuccgcaag aagccccuga acuaccugaa gucccggcgc     3000

gugaacgacu accaccacgc caaggacgcg uaccucaaca ucguggucgg caacguguac     3060

aacaagaagu ucaccagcaa ccccauccag uggaugaaga agaaccggga cacgaacuac     3120

ucccugaaca agguguucga gcacgacguc gugaucaacg gggaggugau cugggagaag     3180

ugcaccuacc acgaggacac caacaccuac gacggcggga cgcuggaccg cauccggaag     3240

aucgucgagc gcgacaacau ccucuacacc gaguacgccu acugcgagaa gggcgagcug     3300

uucaacgcca ccauccagaa caagaacggc aacagcaccg ugucccugaa gaaggggcuc     3360

gacgugaaga aguacggcgg guacuucagc gccaacacgu ccuacuucag ccugaucgag     3420

uucgaggaca agaagggcga ccgggcccgc cacaucaucg gggucccgau cuacaucgcg     3480

aacaugcugg agcacucccc cagcgccuuc cucgaguacu gcgagcagaa gggcuaccag     3540

aacgugcgga uccuggugga gaagaucaag aagaacuccc ugcucaucau caacggcuac     3600

ccccugcgca uccgcgggga gaacgagguc gacaccagcu ucaagcgggc cauccagcug     3660

aagcucgacc agaagaacua cgagcuggug cggaacaucg agaaguuccu ggagaaguac     3720

guggagaaga agggcaacua ccccaucgac gagaaccgcg accacaucac ccacgagaag     3780

augaaccagc ucuacgaggu ccugcugucc aagaugaaga aguucaacaa gaaggggaug     3840

gccgacccga gcgaccggau cgagaagucc aagcccaagu ucaucaagcu cgaggaccug     3900

aucgacaaga ucaacgugau caacaagaug cugaaccucc ugcgcugcga caacgacacc     3960

aaggcggacc ugagccucau cgagcugccc aagaacgccg gcagcuucgu ggucaagaag     4020

aacacgaucg ggaaguccaa gaucauccug gugaaccaga gcgugaccgg ccucuacgag     4080

aaccggcgcg agcuguga                                                   4098


<210>  1928
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1928
auggacaccg ccaagaccaa caagcagccc uacuacaucg gccuggacau ggggacgagc       60

uccgugggcu gggccgucac cgacgcgagc uacaacgugg ugaagaagca cgggaaggcc      120

cucuggggcg uccgccuguu cgaggaggcc cagaccgccg ccgaccggcg ccugcaccgg      180

accgcgcgcc ggcgcaucca gcggcgcaag cagcggcucg cccugcugca ggagcuguuc      240

gccaaggaga ucugcaaggu ggacccgggg uucuucaucc gccugcacga gucccggcug      300

uggcaggagg acaagagcau cgaccagccc aacacccucu ucaacgacga ggacuucgac      360

gacaaggccu accacaaggc guaccccacc aucuaccacc uccgcuacgc ccugaugacc      420

gagaacaagu ccuucgacgu gcggcucguc uaccuggcca uccaccacau caucaagcac      480

cgcggccacu uccugcucga gaacuacgac cugagcaacc aggagaccuc cggcuucgac      540

gagagcuaca ucgcccugac gaacgccauc aaggagcucc ugcagaagga gauccccgug      600

gggcacgugg agaagaucca gaucauccug aaggacaagu cccucagcaa gcgggagaag      660

accgagaaga uccugcaggu cugggacgug aucaaggaca agcagcugaa ggaggugguc      720

cagcucaucu gcggcgggac cgcgaaccug uccacccugu ucauggacga gagccucaag      780

gaggcccuga agguguucga gaucaaggac uccaagauca gcuucuccac ggccguguac      840

gaggagaagg agagcgaccu gcaguccguc cucgagcagc gcuucgaccu gguguacgcc      900

gccaagaugc uguacgacug gagccuccug gcggagcuga ugggcgaccg gaagacccuc      960

uccgaggcca agaucgccau cuacgagacc cacaagaagg accuggaggu gcugaagcgc     1020

cucacccggc aggacaagag ccuguaccgc aagcuguucc gggggacgga caaggacagc     1080

uacuccgccu acgucggcag cugcaugauc cacggcgaga agcucgugau cgagaagcgc     1140

ggguccaccg aggaguucua caaguuccug aagaaggaga ucagcaagcu gccggacugc     1200

gaggacaaga ucuacgugcu cuccaagauc gaggccgaga acuuccugcc caaggcgguc     1260

agcaccauca acggcaucau ccccuaccag cugcaggagc aggagcucga caucauccug     1320

aagaaggccg agcaguaccu gcccuuccug auggagaagg acgccuacgg gaccaucucc     1380

gagaagauca agcagcuccu gacguuccgc aucccguucu acgugggccc ccugaaccgg     1440

cacagcgaca aguccugggc cgugcacacc gacaaggagg gccgcauccu ccccuggaac     1500

uucaacgagc ggaucaacga ggagaagucc gccgagaagu ucaucacccg caugaccaac     1560

aagugcacgu accugcuggg gaaggacguc cuccccaaga acagccugcu guacaccgag     1620

uacaugcugu ucaacgagcu caacaacgug aagaucggca aggcggggac cuaccugacc     1680

gaggagcaga aggagaagcu cuggaaggag cuguuccuga cgaccaagaa ggucacccuc     1740

agcaaguucu ccaaguuccu gaucaaggag ggcaucgaca aggaggaggc cguggagauc     1800

aaggggcugg acggcggcuu caagagcucc cucgccccgu ggaucgaccu gaagaacauc     1860

cugggggagg acuucacccg ggacaaggcc gaggagauca ucaaggcgau cacccucuuc     1920

agcauggaca agaagauccu gaagaaguac cugaugcgca ucguguccga gcccgucgcc     1980

aagcagcuca gcaagcugcg guacacgggc ugggggcgcu ucuccaagga guuccugacc     2040

gagaucacgc cccgggugac ggacgacgug cagguccucg uggacaccag caccggcgag     2100

aucaugaaca ucaucaccgc ccugaagaag accagccuga accucaugga gauccugucc     2160

agcacguacg gguacuccgc cgccaucagc gaggcgaaca aggagcuggu gggcaacacc     2220

guccucuccu acgagaccgu gaaggagcug accgugagcc ccgccgucaa gcgcccgauc     2280

uggcagacgc ugaagaucau caaggagauc acccacauca ugggcgccga gcccgcccgg     2340

aucuucaucg agauggcccg cgacaagcag cccgacaagg ggcggaccga cucccgcaag     2400

aagcagcuca ucgagcugua caagaagugc aaggaggacg ugcggagcug guccaaccgc     2460

gagagcaagg acugggugga ggagaucgag uccaagagcg acucccagcu gcggagcaac     2520

aagcuguucc ucuacuacac ccagaugggc aagugcaugu acacggggaa gcccaucgac     2580

cugggcgcgc uccugaaggg gcagcuguac gaccgcgacc acaucuaccc gcagucccag     2640

accaaggacg acagccucga caaccugguc aucguggaca accgggagaa ccgcgacaag     2700

uccaacaccu acccccugag caagaacauc cgcgagcggc agaucggcuu cuggaagcuc     2760

cugcgcgaca agggcuucau cagcaaggag aaguacuucc ggcugacccg caacacgccc     2820

uucuccgacg ccgagaaggc cgaguucauc gcccggcagc ucguggagac ccgccagagc     2880

accaaggccg ucgcggagau ccugaagcgg gugcugccca aguccaucau cguguacguc     2940

aaggccgggc ucaccagccg cuuccggcag uacggcgagu ucaucaaggu gcgcgaccug     3000

aacgacuacc accacgccaa ggacgccuac cugaacaucg ugacggggaa cguccacuac     3060

accaaguuca ccuccaaccc gauccgguuc gugaaggacu gccagaucua cagccucaac     3120

gagcacgcgc uguacaagua caccguggag cgcaacggcg ucguggccug ggcccccaac     3180

gaggagggca ugaugcccuc cgugacgcgg uggaugaaca agaacaacau ccuggucacc     3240

cgcaugagcu acguggggca gggcgagcug uucgaccagc ucccccugaa gaaggggaag     3300

ggccaggugu cccucaagaa ggagggggcc aucagcgaca ucuccaagua cggcggcuac     3360

aacagcgccu ccaccgcgua cuucauguac gucgaggggg aggacaacaa gggcaagccg     3420

guguacguga ucgagaccau gccccugcac cuggccgccc ggcucacgac ccuggaggag     3480

aagcagaagu acugcgagga gcuguggaug agcaccgggg gcaagcucaa gaaccccaag     3540

guccugaucg agcagauccc gauccagucc cuguucagcg uggacggguu ccgcgugacc     3600

aucgccggca agacgggcaa gcaguacggg ugccggcucg ccgaggagcu gguccugaac     3660

aacggcuacg cgaugacccu caaggccgug cugaaguucg ugaaccgccg gaagaugaac     3720

aaggaggccc ugaucaccuc cuacgaccac cucgaggaga gccagcugcu ggcccucuac     3780

gacgucuucc uggacaagcu ggagcacucc ugcuucagca agcgcuucag cacccagcgg     3840

gacgugcuca agaacgggcg cgaguccuac aucgcccuga gccuggagga caagugccag     3900

auccucuccg agauccugca ccuguuccag ugcaacgcga cgcucagcaa ccugucccug     3960

cucggcagcu ccaagcagag cgggcgccug uacaugucca agaaccugag cgacaaggac     4020

uccugguaca ucauccacca gagcgugacc ggcuucuacg agcagcacau cccccuggcc     4080

cccuacaaga aguccuga                                                   4098


<210>  1929
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1929
auggugaaga agaaguacgc caucggcauc gacaucggga ccaacagcgu cggcuggucc       60

guggugaccg acgacuacaa gguccccagc aagaagauga agguguucgg gaacacggag      120

aagcgcuaca ucaagaagaa ccugcucggc acccugcugu ucgacgaggg gaacaccgcc      180

gagaaccggc gccucaagcg gaccgcgcgc cggcgcuaca cgcggcgccg gaaccgcauc      240

cuguaccugc aggagaucuu cgccgaggag aucaacaaga ucgacgacuc cuucuuccag      300

cggcucgacg acagcuuccu gaucguggag gacaagcagg gcuccaagca cccgaucuuc      360

ggcacccugc aggaggagaa gaaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacagcuc ccagaaggcc gacauccggc ugaucuaccu cgcccuggcg      480

cacaucauca aguaccgcgg gcacuuccug uucgagggcg accucaagag cgagaacaag      540

gacguccagc accuguucaa cgacuucgug gagauguucg acaagaccgu ggaggggucc      600

uaccugagcg agaaccuccc caacgucgcc gacgugcugg uggagaaggu cuccaagagc      660

cggcgccugg agaacauccu ccacuacuuc cccaacgaga agaagaacgg ccuguucggg      720

aacuuccugg cccucgcccu gggccugcag ccgaacuuca agacgaacuu cgagcucgcg      780

gaggacgcca agauccaguu cuccaaggag accuacgagg aggaccugga ggagcugcuc      840

ggcaagaucg gggacgacua cgccgaccug uucaucgcca ccaagagccu guacgacggc      900

auccuccugg ccgggauccu guccaccacg gacagcacca ccaaggcgcc ccucuccagc      960

uccaugguga accgguacga ggagcacaag aaggaccugg cccugcucaa gaacuucauc     1020

caccagaacc ugagcgacag cuacaaggag guguucaacg acaagcugaa ggacggcuac     1080

gccggcuaca ucgaggggaa gaccacgcag gagaacuucu accgcuucau caagaaggcc     1140

aucgagaaga ucgagggcuc cgacuacuuc aucgacaaga ucgaccggga ggacuuccuc     1200

cgcaagcagc ggaccuucga caacgggagc aucccccacc agauccaccu ccaggagaug     1260

cacgccauca uccgccgcca ggcggaguuc uaccccuucc uggucgagaa ccaggacaag     1320

aucgagaaga uccucaccuu ccggaucccg uacuacgugg gcccccuggc ccgcgggaag     1380

uccgaguucg ccuggcugaa ccgcaagagc gacgagaaga uccggcccug gaacuucgac     1440

gagauggugg acaaggagac cuccgccgag aacuucauca cgcggaugac caacuacgac     1500

caguaccucc ccgaccagaa gguccugccg aagcacagcc ugcuguacga gaaguucgcc     1560

guguacaacg agcucaccaa ggugaaguuc aucgcggagg gcaugcgcga cuaccaguuc     1620

cuggacuccg gccagaagaa ggacaucguc aagacccugu ucaagacgaa gcggaaggug     1680

accgccaagg acaucaaggc cuaccucgag aacagcaacg gguacgccgg cguggagcug     1740

aaggggcugg aggagcaguu caacgccucc cuccccaccu accacgaccu gcugaagauc     1800

cuccgcgaca aggcguucau cgacgccgag gagaaccagg agauccugga ggacaucguc     1860

cugacccuca cgcuguucga ggaccgggag augauccgca agcggcugga gaaguacaag     1920

gacauccuca ccgaggagca gcgcaagaag cuggagcggc gccacuacac cggcuggggg     1980

cggcugagcg ccaagcucau caacggcauc cuggacaagg ugacccgcaa gacgauccug     2040

ggcuaccuca ucgacgacgg gaccuccaac cggaacuuca ugcagcugau caacgacgac     2100

acccugagcu ucgucgacga gauccgccuc gcccagggcu ccggggaggc ggaggacuac     2160

cgggccgagg ugcagaaccu ggccggcagc cccgccauca agaaggggau ccugcagucc     2220

cucaagaucg uggacgagcu gaucgagguc augggcuacg accccgagca caucguggug     2280

gagauggccc gcgagaacca guucaccaac cagggccgcc ggaacagcca gcagcgcuac     2340

aagaagaucg agaacgcgau caagaaccug gacuccaacc ucaacagcaa gauccugaag     2400

gaguacccga cgaacaacca ggcccugcag aacgaccggc uguuccugua cuaccugcag     2460

aacgggaagg acauguacac cgacgaggag cucgacaucg accagcuguc ccaguacgac     2520

aucgaccaca ucauccccca ggccuucauc aaggacgaca gccuggacaa caagguccuc     2580

accaagagcg ccaagaaccg cggcaagucc gacgacgugc ccagccugga gaucgugcac     2640

aagaagaaga acuucuggaa gcagcugcuc gacucccagc ugaucagcca gcggaaguuc     2700

gacaaccuga ccaaggccga gcgcgggggc cucaccaacg aggacaaggc gcgguucauc     2760

cagcgccagc uggucgagac gcggcagauc accaagcacg uggcccgcau ccuggacacc     2820

cgguucaaca ccaagcucga cgaggccggc aaccgcaucc gggaccccaa ggugaacauc     2880

aucacgcuga aguccaaccu ggucagccag uuccgcaagg acuaccagcu cuacaaggug     2940

cgggagauca acaacuacca ccacgcccac gacgccuacc ugaacgcggu ggucgccacc     3000

gcccugcuca agaaguaccc gcagcuggcc cccgaguucg uguacgggga cuaccccaag     3060

uacaacuccu acaagagccg caaguccgcg accgagaagg ugcuguucua cagcaacauc     3120

augaacuucu uccggcgcgu ccucguguac uccaagaccg gcgaggugcg gauccgcccc     3180

gucaucgagg ugaacaagga gacgggggag aucguguggg acaagaaguc cgacuuccgg     3240

accguccgca aggugcugag cuacccccag gugaacgucg ugaagaaggu ggagaugcag     3300

accggcgggu ucagcaagga guccauccug cagcacggcg acagcgacaa gcucaucccc     3360

cgcaagaccg agaaguucua ccuggacacg aagaaguacg gcggguucga cucccccacc     3420

aucgccuaca gcguccugcu caucgccgac aucgagaagg gcaaggccaa gaagcugaag     3480

cgggugaagg agcugaucgg gaucaccauc auggagcgca uggccuucga gaagaacccc     3540

aucgaguucc ucgagcacaa gggcuacaag aacauccugg agaagaacau caucaagcug     3600

ccgaaguaca gccucuucga gcuggagaac gggcggcgcc ggcugcucgc guccgccaag     3660

gagcugcaga agggcaacga gaugauccug ccgccccacc ucgugacccu gcuguaccac     3720

agcuccaaca uccacaagau cacggagccg auccaccuca acuacgucaa caagaacaag     3780

cacgaguuca aggagcugcu gcgccacauc agcgacuucu ccacccggua cauccucgcc     3840

caggaccgcc ugagcaagau cgaggagcug uacgacaaga acgacggcga cgacaucucc     3900

gaccucacca gcuccuucgu gaaccugcug accuucacgg ccaucggggc ccccgcggcc     3960

uucaaguucc ucggcagcgu gaucgaccgg aagcgcuaca ccuccaucgc cgagauccug     4020

gaggccaccc ugauccacca gagcgucacc gggcucuacg agacgcggau cgaccugucc     4080

aagcugggcg gggacuga                                                   4098


<210>  1930
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1930
augaagaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gccggccaag aagaugaagg ugcucgggaa cacgaacaag      120

aagaccauca agaagaaccu gaucggcgcg cugcuguucg acgccgggaa caccgccgcc      180

gaccgccggc ugaagcgcac cgcccggcgc cgguacacgc gccggcgcaa ccggauccug      240

uaccuccagg agaucuucgc ggccgagaug aacaagcugg acgagagcuu cuuccaccgc      300

cuggacgacu ccuuccucgu ccccgaggac aagggcggca gcaaguaccc caucuucggg      360

acccuggagg aggagaagga guaccacaag caguucccca ccaucuacca ccuccggaag      420

ucccucgccg acagcaagga gaaggccgac cugcgccuga ucuaccucgc gcuggcccac      480

aucaucaagu accggggcca cuuccuguac gaggacaccu ucgacaucaa gaacaacgac      540

auccagaaga ucuucaacga guucacgucc aucuacaaca acaucuucga ggagagcucc      600

cucagcaacg agaacgccca gguggaggag aucuucaccg acaagaucuc caagagcgcc      660

aagaaggacc gcgugcugaa gcuguucccg gacgagaagu ccaccgggcu guucagcgag      720

uuccugaagc ugaucgucgg caacaaggcc gacuucaaga agcacuucga ccucgaggag      780

auggcgcccc ugcaguucuc caaggacacc uacgacgagg accuggagag ccuccugggg      840

cagaucggcg acgacuacgc cgaccuguuc gugguggcca agaagcucua cgacgccauc      900

cugcuggccg gcauccucuc cgucaaggac cccgcgacga aggcgccccu gagcgccucc      960

augaucgagc gguacgacaa ccaccagaac gaccugagcg cccucaagca guucauccgc     1020

agcaaccugc cggagaagua cgccgaggug uucuccgacg acagcaagga cggguacgcc     1080

ggcuacaucg acgggaagac cacccaggag ggcuucuaca aguacaucaa gaaccagauc     1140

uccaaguucg agggcaccaa guacuuccug gagaagaucg agcgggagga cuuccuccgc     1200

aagcagcgga cguucgacaa cgggagcauc ccccaccaga uccaccucca ggagaugaac     1260

gccauccugc agaaccaggg cggguacuac cccuuccuca aggccaacaa ggagcgcauc     1320

gagcagaucc ugaccuuccg gauccccuac uacgugggcc cgcuggcccg cgggaacucc     1380

gacuucgccu ggcucacccg caacagcgac gacgcgaucc ggcccuggaa cuucgaggag     1440

gucguggaca aggccuccag cgccgaggac uucaucaaca agaugaccaa cuacgaccug     1500

uaccugcccg aggagaaggu gcuccccaag cacucccugc uguacgagac guucgccguc     1560

uacaacgagc ugaccaaggu gaaguucauc gcggaggcca ugaaggacua cgaguuccuc     1620

gacagcggcc agaagcgcaa gaucguggag aacaucuuca agaaccaccg gaaggucacc     1680

gagaaggaca ucaucaacua ccugcacaac guggacggcu acgacgggau cgagaucaag     1740

ggcaucgaga agcaguucaa cgccucccug agcaccuacc acgaccuccu gaagaucauc     1800

aaggacaagg aguucaugga caacccgaag aacggggaga uccuggaguc caucauccac     1860

acgcucacca ucuucgagga ccgcgagaug aucaagcagc ugcuggccca guacaacagc     1920

guguucgacg agaaggucau caaggcccuc ucccggaagc acuacaccgg cugggggaag     1980

cugagcgcga agcugaucaa cggcauccgc gacaagcaga ccggcaacuc cauccucgac     2040

uaccugaugg acgacggcaa gagcaaccgg aacuucaugc agcugaucaa ggacgacggg     2100

cucuccuuca aggagaucau cgacaaggcc cagguggugg gggagacgga caaccugcgc     2160

caggucgugc agaaccugcc cggcagcccc gccaucaaga aggggauccu ccagagcauc     2220

aagcuggucg acgagcuggu gaaggugaug ggcuccgacc ccgagagcau cgucaucgag     2280

auggcccggg agaaccagac caccaacaag ggcaagucca agagccagca gcgccucaag     2340

acccuguccg acgccaucag cgagcugggg uccaacaucc ucaaggagca cccgacggac     2400

aacauccagc ugcagaacga ccggcuguuc cucuacuacc ugcagaacgg caaggacaug     2460

uacaccgggg aggcgcugga caucaaccag cucagcaacu acgacaucga ccacaucauc     2520

ccccaggccu ucaucaagga cgacucccug gacaacagcg ugcugaccuc cagcaaggac     2580

aaccgcggca aguccgacaa cgugcccagc cucgagaucg ucgagaagau gaagggcuuc     2640

uggcagcagc ugcuggacuc caagcucauc agcgagcgga aguucaacaa ccugaccaag     2700

guggagcgcg ggggccugga cgagcgggac aagguggggu ucaucaagcg ccagcucguc     2760

gagacgcggc agaucaccaa gcacguggcc cagauccugg acgcccgcuu caacgccgag     2820

gugaacgaga agaaccagaa gcugcggucc gucaagauca ucacccucaa gagcaaccug     2880

gugagcaacu uccgcaagga guucggccug uacaaggugc gcgagaucaa cgacuaccac     2940

cacgcgcacg acgccuaccu caacgccguc aucgccaagg cgauccugaa ggaguacccc     3000

aagcuggagc cggaguucgu guacggggac uaccagaagu acgaccucaa gcgguacauc     3060

ucccgcacca aggaccccaa ggagaucgag aaggccaccg agaaguacuu cuucuacagc     3120

aaccugcuga acuucuucaa ggacaaggug uacuacgccg acggcacgau cauccagcgg     3180

ggcaacgucg aguacuccaa ggacaccggg gagaucgccu ggaacaagaa gcgcgacuuc     3240

gccaucgugc ggaaggugcu cagcuacccc caggucaaca ucgugaagaa gaccgaggag     3300

cagaccggcg gguucuccaa ggagagcauc cugcccaagg gcaacuccga caagcugauc     3360

ccgcgcaaga cgaagaacau ccagcucgac accaccaagu acgggggcuu cgacagcccc     3420

gugaucgcgu acuccauccu gcuggucgcc gacguggaga agggcaagag caagaagcuc     3480

aagaccguga agucccugau cgggaucacg aucauggaga aggucaaguu cgaggccaac     3540

cccguggccu uccuggaggg caagggguac cagaacgugg ucgaggagaa caucauccgg     3600

cucccgaagu acagccuguu cgagcuggag aacggccgcc ggcgcaugcu cgccuccagc     3660

aaggagcugc agaaggggaa cgagauggug cugcccuccu accuccuggc gcugcucuac     3720

cacgccaaga agauccagaa gaaggacgag cccgagcacc uggaguacau caagcagcac     3780

cacagcgagu ucaacgaccu gcucaacuuc guguccgacu ucagccagaa guacguccug     3840

gccgagucca accuggagaa gaucaagaac cucuacaucg acaacgagca gaccaaccug     3900

gaggagaucg ccaacagcuu caucaaccug cucaccuuca ccgccuucgg cgcgcccgcc     3960

guguucaagu ucuucggcaa ggacaucgag cggaagcgcu acagcacggu gaccgagauc     4020

cugaaggcca cccugaucca ccaguccauc accgggcucu acgagacgcg gaucgaccug     4080

agcaagcugg gcgaggagug a                                               4101


<210>  1931
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1931
auggacaaga aguacaacau cggccuggac aucgggacca gcuccaucgg cuucguggcc       60

accgacgagg acaaccacgu cauccgcgcc aaggggaaga acgugaucgg cgugcggcug      120

uucgacgagg ggcagagcgc ggccgagcgc cggggcuucc gcacgacccg gcgccggcug      180

aaccgccgga aguggcgccu gaaccuccug cgggagaucu ucgagcccca caucuacccg      240

aucgacgccu ccuucuucau gcgccagaag uacagcaacc uguccaagca ggaccccaac      300

cagaagaucg aguucaagga cauccucuuc agcgacaagu ccgacgccga guucuaccgg      360

caguacccca ccaucuacca ccuccgccag gcccugauga ccgagaagcg gcaguucgac      420

cuccgcgaga ucuaccuggc gauccaccac aucgucaagu accggggcca cuuccuggac      480

gagacgccca ccgacaaguu cagcuucgac cagcucgacc ugacccagga guucgagcgc      540

cugaacgucc ucuucuccga gcagaacccg gaggagcccu ucgagaucgu ggugaaccag      600

aaccagagca ucgaggacau ccuguccaac cagagcauga ccgccuccga ccggcgcaag      660

caggcccugg ugguguucua ccagcagagc gagaacaagg agaacgagaa gcaguacaag      720

gccaucgcga cggagauccu caaccugcug cucgggaaca aggccaagcu gaacgucauc      780

cuggcccagg agguggacaa ggaggccgcc aaggacuggu cccucagccu guccagcgag      840

accuucgacg acgaccugga gaaggcgucc agcggccuca acgacagcca gcuggagaug      900

guggggaucu ucgagcggau cuacuccagc cugauccuca aguccaucau cggcgaccac      960

aacagccugu ccgccgccau ggucgacaag uucaagaccc acgcccagca ccgcgaccug     1020

cucaaggacg ugaucaagaa cagcgacgcc accaaggggg agcagcugca guucgcguac     1080

gaccacuacc ugcagcgcca cuccgccgac ggcaaggccu acggcaagga cgacuucuac     1140

aaggagguga agaagaaccu cgacgacagc gacgccgccc agcagaucca gcagcugauc     1200

gacacggacc aguucaugcc caagcagcgg accaaggcga acggggccau cccccaccag     1260

guccagcaga aggagcugga ccucaucauc gagaaccagu gccaguacua cccguggcug     1320

gccgaggaga accccaacuc cgagcgccac aacguggcca aguacaagcu ggacgagcuc     1380

gugagcuucc ggguccccua cuacgugggc ccccugaucu ccccggccga cgacgagacc     1440

gacccccagg cgauccagaa caccaaguuc gccuggauga uccgcaagga cgcccagggg     1500

cggaucacgc ccuggaacuu cgacgacaag guggaccgcg agaaguccgc cgacaaguuc     1560

aucaagcgga ugaccaccac cgacacguac cugaucggcg aggacguccu ccccgaccag     1620

agccugaucu accagcgcua caaggugcug aacgagcuca acaacgugcg gaucaacggc     1680

cgcccgaucg agguccagau caagcagaac aucuacaccg accuguucaa gaaguucaag     1740

agcguguccg ucaagaagcu gcggagcuac cuggaggaga uguccuacua ccccgggagc     1800

uucaagaucu ccggccucac caaccccggg aaguucgaca acggccugac cagcuaccug     1860

gacuacaaga acauccucgg ggacagcgug gacgcgaacg acaagcgcgc cgacaucgag     1920

cagaugaucg agugguccac gaucuucgag gacaacaaga ucuucgccca gaagcuggag     1980

agcaugaccu ggcugaccgc cgagcagcgg aaggccaucu cccgcaagcg guacaccggc     2040

uggggccgcc ucagcaacaa gcugcugacg cagcucaaga accaggacaa ccgguccauc     2100

auggaccugc ugugggagac cgaggagaac uucaugaaga uccagagcgc gcccgacuuc     2160

uccaagauga ucgaggccca caaccagcag uucaucaaga acgaggccaa cauccagcag     2220

gucaucagcg accucuacac cuccccgcag aacaagaagg ccauccgcca gauccugcug     2280

gugguggacg acauccagaa ggccgugggg cacgcgcccg agcacaucgc caucgaguuc     2340

gccaagggcg aggacaagaa cccccggcgc accaucagcc gcgagaugaa gauccaggac     2400

gucuaccagc agaucuccga cgacauccuc gugagccccg aggugcgggc ccagcugaac     2460

gagaagaucg agaaccgcca cgggcggcug uccgaccgcc ucuuccugua cuucaugcag     2520

ggcgggcggg acauguacac gggcaagccg aucaacauca acaacaucag ccacuacgac     2580

aucgaccaca uccugcccca ggccuucauc aaggacgacu cccucgacaa ccgcguccug     2640

gugagcgaca agcugaaccg ggagaagucc gacaccgugc cccgcgaccu cuucggccgg     2700

gagaugauga gcaccuggga gaagauggcg gaccgcgggc ugaucaccaa gcggaagcug     2760

gccaaccuca agacgauccc cggcagcauc gacgcccaca aggcccaggg guucaucaac     2820

cgccagcugg ucgagacccg ccaggugauc aagcuggcgg ugggcaucau gaucgaccag     2880

uacccggaga cccagaucgu cuccgugaag gccaaccuca cccacgagau gcgggagaug     2940

uucgaccugg ugaagaaccg ggacgucaac gacuaccacc acgccuucga cgccuaccug     3000

accgccuucg uggggaacua ccuccugcag cgcuacccca agcuggagcc cuacuucgug     3060

uacgacaagu ucaagaagcu caaggaccag aacaucagca ugcgggacuu caaccugcug     3120

uacgaccuca agaagaacga ggacgucuac gaccagcgca cgggccacuc cgcgcacaag     3180

aucugggacc agcacggcag caucgacuac auccggcacc uguucgccua caagaagauc     3240

cugguguccc acgaggugca ccagaacggg ggcgcccucu acaacaugac cgucuacggg     3300

aaggccgacg acgccaagcg caagcugauc ccgaucaagg cggacaagcc caccgagcug     3360

uacggcggcu acaccagccg gaagcucgcc uacauggcca ucgugaagac gaagaagggg     3420

aagaaggagc aguaccaggu gaccggcguc cccguguccg cccugaccac ccugcgccag     3480

uucaaggagg acagccccga gcagaagcag gcccucgagg agaucgugcg ccggaacuuc     3540

gacgagaaga cggaggucga gaucaucgug ccgaaggugc uguucaacca gcugaucguc     3600

gacgagaagu gccgcuucau gguggggucc gacaccuacc gguacaacgc gcagcagcuc     3660

gugcugagcg ccgacgaccu ggagaucauc aacgccuccc acaaggacau ccagaccauc     3720

gacagcuccg acuacagcca gcagcucgac caggucuacc agaacauccu gaccaucgug     3780

gacaagcacu uccccaugua cgacaucaac aaguuccgca cgaagcugca cgacaucuac     3840

gagcaguaca ucgagcuccc caacgaggac gugugggaga acaacaagcu gaccacccug     3900

ggcaagcaca ccaucaucau ccggauccuc caggggcugc acgccaacgc gacggucgug     3960

gaccugaagc agcucggcau caagaccccc uucggcuuca ugcagcaggc ccgcgggauc     4020

guggucuccc cggacgccca gcugaucuac cagagcccca ccggccugua cgagcggcgc     4080

gugcggcucg gggaccugug a                                               4101


<210>  1932
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1932
augggcgacc gcaaguacaa ccuggggcuc gacaucggca ccagcuccau cggguucgcc       60

gccguggacg agaacaacca gcccauccgg gucaagggca agaccgcgau cggggugcgc      120

cuguucgagg agggcaagac ggccgccgac cggcgcggcu uccggaccac ccgccggcgc      180

cugagccggc gccgguggcg caucaaccuc cugaacggga ucuucgacgc ccaccuggcc      240

gagguggacc cgaccuuccu cgcgcggcug aaggagucca accgcagcaa ccuggacccc      300

aagaaguccu uccagggcag ccuccuguuc cccgagcgga aggacuacca guucuacaag      360

gaguacccca cgaucuacca ccuccgcaag gcccucaugg aggaggaccg gaaguucgac      420

auccgcgaga ucuaccuggc cguccaccac aucaucaagu accgggggaa cuuccugaac      480

ggcaccccga ugcgcuccuu caagguggag aacaucgagc ucgacacccu guucgaccgg      540

cugaaccagc ucuacgagga gaucaucccc gacaacgagc uggccuucga ccuggagcag      600

gucgcggacg ugaaggaggu gcucagcucc accacgaucu acaagaugga caagaagaag      660

cagcugguga agaugaugcu gcuccccacc agcaacaagg cccugcaguc cgagaacaag      720

aagaucguca cccaguucgu gaacgccauc cugaacuaca aguucaagcu cgacgugcug      780

cugcaggucg agaccgacgc cgacuggagc cucaagcuga acgacgaggg ggccgacgac      840

aagcuggagg aguucacggg cgaccucgac gagaaccgcc uggagaucau cgaccugcuc      900

cagcgccugc acaacugguu cucccugaac gagaucacca aggacggcaa cagccucucc      960

gcggccaugg uggagaagua caaggaccac cggcaccacc uggagcugcu caagaaggug     1020

aucgaggacc accccgacgc caagaaggcc aaggcccuga aggagaccua caccgcguac     1080

gucgggaaga cggacgacaa gacccagaac caggacgacu ucuacaaggc cguggagaag     1140

aaccuggacg acagcccgga cgccaaggag aucaagcgcc ucauccagcu ggaccaguuc     1200

augcccaagc agcggaccgg ccagaacggg gccauccccc accagcugca ccagcaggag     1260

cucgaccaga ucaucgagaa gcaguccaag uacuaccccu uccuggccga gccgaacccc     1320

aacgugaagc gccggaagga cgcgcccuac aagcuggacg agcugaucgc cuucaagauc     1380

cccuacuacg ucggcccgcu cgugaccccc gaggagcagg cccagaacgg cgagaacgug     1440

uucgccugga ugaagcgcaa ggucgccggg cccaucacgc ccuggaacuu cgacaagaag     1500

guggaccgga uggagagcgc gaaccgcuuc auccggcgca ugaccaccaa ggacaccuac     1560

cuguucggcg aggacgugcu gccggccgag uccaugaucu acgagaaguu cgucgugcuc     1620

aacgagcuga acaaccugaa gaucaacggg cggcaccuca gccugaagga caagcaggac     1680

aucuacaacg accuguucaa gcagcagaag acggucagca ucaaggcccu ccagaacuac     1740

uacgugacca agaagaaggc cgcgaccgcc cccaccgugg gcgggcuggc cgaccccaag     1800

aaguuccugu ccagccucuc cacguacauc gacuucaaga acauguucgg cgagcagguc     1860

aacgaccccc aguuccagga ggaccuggag cagaucgugg aguggagcac caucuucgag     1920

gaccgcggca ucuucaaggc caagcugcag gcccucgggu ggcuguccga gaagcagauc     1980

cagcagcugg uggcgaagcg guacaagggc ugggggcgcc ucagcaagaa gcugcugacc     2040

ggccucaaga acgccgaggg guacuccauc cuggacgaga uguggcggag caccgagaac     2100

uucaugcaga uccagucccg cccggaguuc gccgcccuga uccagcaggc caacgagaag     2160

caguucgagg gcaacgaccc caacaacguc ugggagaaca ucgagaacau ccucggcgac     2220

gcguacacga gcccccagaa caagaaggcc auccgccagg uggugaaggu cgugcaggac     2280

aucgagaagg ccguggggaa cccgcccgag aagaucgcca ucgaguucac ccgggaggcc     2340

gcggccaacc cccagcggac ccagucccgc cugcgcaccc uggagaagcu cuacgagagc     2400

gccgaggagg ucguggacgc ccggcugacg gcggagcugg ccgaguucaa ggagaacaag     2460

cacgugcucu ccgacaagua cuaccuguac uucacccagc ugggccgcga cgucuacacc     2520

ggggacacca ucagccucga ccggcugaac gacuacgacg uggaccacau ccugccccag     2580

uccuucauca aggacgacag ccucgacaac cgcgugcuga ccauccgggc cgucaacaac     2640

ggcaaguccg acaacgugcc ggccaagaug uucgggaaga agaugggcag cuucuggcgc     2700

uaccugcucg acaacggcau gaucuccaag cggaaguaca acaaccugau cacggacccc     2760

gacaacauca gcaaguacgc ccagaagggg uucaucaacc gccagcuggu ggagaccagc     2820

caggucauca agcucaccgc gaacauccug aacggcaucu acgacaagga caccgagauc     2880

aucgaggugc ccgccaagau gaacucccag augcggaaga uguucgaccu ggugaagguc     2940

cgcgagguga acgacuacca ccacgccuuc gacgccuacc ucacgaucuu caucgggaac     3000

uaccuguaca agugcuaccc caagcugcgg ccguacuucg uguacggcaa guucaagaag     3060

uucagcgaca acgaggacau cgaggucggc gacgggcgcu ucaacuuccu caaccggauc     3120

gagcgccuga agaacgugac cgaccccgag accggcgaga uccugugguc caacguggcc     3180

cccaacgaga ccaucaagcg gaucaagaag gucuacaacu acaaguucau gaucgugagc     3240

cgcgagauca cgacccggcg cgcggagcug uucaaccaga ccguguaccc caagaacuac     3300

cgggggaagc ugaucccgau caaggaggac cgccccaccg accuguacgg cggguacucc     3360

ggcaacacgg acgccuaccu cgccaucguc gcccuggagg acaagaagaa gggcaaguac     3420

uucaaggugg uggggauccc cacccgggcc gcggccaagc uggagaagcu caagcagcag     3480

gacagccagc aguaccugca ggccguccac gaggugaucg cgccccaguu caccaagucc     3540

accaagaagg gcaucaagaa gacggaguuc cgcaucgugc uggacaaggu ccacuaccgc     3600

cagcucgugc aggacgggcc ggugaagaug augcugggca gcuccaccua caaguacaac     3660

gccaagcagc ugguccucag cgagaaggcc cuccagguga ucaaggacga ccggaaguuc     3720

gacgagaccc agaaggacga caaccugauc gccguguacg acgagauccu guccaucguc     3780

aaccagagcu ucgaccugua cgacaucaac ggguuccgca agaagcugaa cgacaaccgg     3840

aaccaguuca ucgaccuccc cgccgagacc aaguacgagg gccgcaaggu gguggcccac     3900

ggcaagcggg agaugauccu ggagauccug aaggggcucc acgcgaacgc cuccuucggc     3960

aaccugaagc ccaucggguu cgccacgccc uucggccaga ugcagagcaa gaacgggauc     4020

gagcuguccg agaacgccuu ccucauccac cagagcccgu ccggccuguu cgagcacaag     4080

aucaagcuga gcgaccucug a                                               4101


<210>  1933
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1933
augaccaagc ugggcaagcc cuacgggauc ggcguggaca ucgggagcaa cuccaucggc       60

uucgccgccg ucgacgagaa cagccaccuc auccgccuga aggggaagac cgugaucggc      120

gcgcggcugu ucgaggaggg caaggccgcc gccuaccgcc gggccucccg cacgacccgg      180

cgccggcuca gccgcaaccg guggcgccug uccuuccugc gggacuucuu cgagagccac      240

aucaccccga ccgaccccaa cuucuucaug cgccagaagu acuccgagau cagccccaag      300

gacaaggcgc gguacaagua cgagaagcgc cucuucaacg accggacgga cgccgaguuc      360

uaccagcagu accccaccau guaccaccuc cgcacccggc ugcucaccga cccguccaag      420

gccgacgugc gcgagaucua cuucgccauc caccacaucc ucaagagccg ggggcacuuc      480

cugacgcccg gcuacgcgaa ggacuucaac accaacaagg ucgcccugaa cgagaucuuc      540

cccgcccugc aggacgccua cgcccaggug uaccccgacc uggacaucac cuucgacgag      600

aacaagguga acgaguucaa gaccguccuc cugaacgaga aggcgacgcc guccgacacc      660

cagcgcgccc uggugaaccu ccugcuggcc aaggaggggg accgggacau ccucaagcag      720

cagaagcagg uccugaccga guucgccaag gccguggugg gccugaagac caagcucaac      780

guggcgcugg ggacggaggu cgacagcucc gaggccaccg ccuggaacuu cagccugggc      840

cagcucgacg acaagugggc cggcaucgag uccgccauga ccgacgaggg gaccgagauc      900

cuggaccaga uccgcgaccu guaccgcgcg cggcuccuga acggcaucgu gcccgccggg      960

aagacgcuga gccaggccaa gguggacgac uacgcccagc accacgagga ccucgagcug     1020

uucaaggacu accugaagca gcucgaggac gacggcaccg ccaaggcgau ccgccagcug     1080

uacgaccggu acaucgacgg cgacgacgcc aagcccuucg uccgcgagga cuucgugaag     1140

gcccugacca aggaggugac cgcccacccc aacgcgaagu ccccggagcu ccuggagcgg     1200

cuggcccagc ccgacuucau gcucaagcag cgcaacaagg ccaacggggc cauccccguc     1260

cagaugcagc agcgggagcu ggaccagauc aucaagaacc aggccgugua cuacgacugg     1320

cuggcggccc ccaacccggu ggagaagcac cgccagagca ugacguacca gcucgacgag     1380

cugcugaacu uccggauccc cuacuacguc ggcccccucg ugaccgccaa ggagcagaag     1440

gccgcccagg gcgggguguu cgcguggaug guccgcaagg accccgacgg gaacaucacc     1500

ccguacaacu ucgacgagaa gguggaccgg gaggccuccg ccaacaccuu cauccagcgc     1560

augaagacga ccgacaccua ccugaucggc gaggacgucc ugcccaagca gagccugcuc     1620

uaccagagcu acgaggugcu gaacgagcug aacaacgugc gggucaacga caacaagcuc     1680

uccuucgacc agaagcagca gguccugggc gagcuguucg agcgccacaa caccgugacg     1740

aucaagcagu ucguggacaa ccuccuggcc cacggggacu acgaccggau ccccgaggug     1800

cgcggccugg ccgacgagaa gcgguuccuc agcucccuga gcaccuacaa ccagcugaag     1860

gggauccucc acgaggcgau cgacgacgcc gacaagcaga ccgacaucga gaacaucauc     1920

gccuggucca ccaucuucga ggacaaggcc aucuucaaga cgaagcugaa cgagaucagc     1980

uggcugaacg cgggcaugau caacucccuc gccaacaucc gcuaccgggg guggggccag     2040

uucagccaca agcugcugaa cgagcucacc cuguccaccg gccgcaccgu guucgaggag     2100

cugcucauca gcucccgcaa ccugaugcag auccugaccg acgagguccu caagcagaag     2160

aucgccgagc ugaacgccca cgagcugaag gccagcggga ucuacgcggc caucgacgac     2220

gccuacacgu cccccagcaa caagaaggcc cuccgccagg ugcuguccgu gaucgacgac     2280

aucaccaacg ccgcgggcca ggccccgaac uggcuguaca ucgagaccgc cgacggggac     2340

ggcguccccg ggaagcggac caacagccgg cagcgcaaga uccaggagau cuacgccaac     2400

gccgcgcagg agcucaucac gggcuccgug cggggcgagu ucgaggacaa gaucgccagc     2460

cgcgccgacu ucaccgaccg gcuggugcug uacuucaugc agggcgggcg cgacaucuac     2520

accggggagc cccucaacau cgacaaccug uccagcuacg acaucgacca cauccugccc     2580

cagagccuca ucaaggacaa cucccuggac aaccgggucc uggugcaccc gcucgugaac     2640

cgcgagaagg acaccaucuu cgccagcacg cgguucgcga agcagaugaa cggcaugugg     2700

cgccaguggc acgccgccgg ccugaucucc agccggaagc ugcgcaaccu ccagaugcgg     2760

cccgacgaga ucgacaagua cgccuccggg uucgucgccc gccagcuggu ggagacccgg     2820

cagaucauca agcugaccga gcagaucgug gcggaccagu accccgacac caagaucauc     2880

gccgucaagg ccggccucag cucccagcug cgcaaggagc uggacuuccc gaagaaccgg     2940

gaggugaacc acuaccacca cgccuucgac gccuuccucg cggcccgcau cgggacguac     3000

cugcugaagc gguaccccaa ccucgagccc uucuucaccu acggcaaguu caagaagacc     3060

gaggugaaga agcucaagag ccugaagucc cucaagagcu ucaacuucau ccgcgacaug     3120

acccacgcca aggacaagau cgucgccaag gagacggggg agaucgugug ggacaacgcc     3180

uccgacauca acgagcugga ccgcaucuac aacuucaagc ggaugcugau cacccacgag     3240

gugcgcuucg agaccgcgag ccuguucaag cagacccugu acgccgccaa gaacuccaag     3300

aaccggggcg gcagccgcca gcugaucccc aagaagaagg gguaccucgu cgacaucuac     3360

ggcggguaca cgcaggagac cggcuccuac cugagcgugg ugcggcugac caagaaggcc     3420

auguacgccg ucgugaaggu guccacccgc gacgcggcca agcucgccgu cgccaagagc     3480

aucagcgagc agaaggagaa cgagacgcug aagaagauca ucgacgggaa gcuguccaag     3540

accagcaaga agggcaagac cacccaccag cuguucgaga ucgugcugcc gcgggugggc     3600

cagaagacgc uguucaagaa cuccaaguac aaccaguucc ucgucaacag cgacaccuac     3660

augcacaacu accaggagcu guggaugccc cgcgaguacc agcggaugug gaaggacauc     3720

cugcucucca accacgggga cgcgcagauc gagggccagc uggaccagau cuucaaguuc     3780

aucgugagcc aggugaacuc cuacuucaac cuguacgaca ucaaccaguu ccgcaagaag     3840

aucgccgacg ccaccgacaa guucgccaag cuccccaccc acgacacgga caacgcccgg     3900

gggaagaucg cgaccaucgg ccagcugcug aucgggcucc aggccaacgc caugcgcagc     3960

gaccugcgga accuggacau caagaccccc uucggccucc ugcagaucgg caccgggauc     4020

acgcuggacc ucgacaccuc caucaucuac cagagcccga ccggccuguu cgagcgcgag     4080

gucccccuga ccgaccucug a                                               4101


<210>  1934
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1934
augaccaagg acuacagccu gggccucgac aucgggaccg gcuccguggg guucgccgcc       60

cuggacacga acuaccgccu gcuccgggcg aagggcaaca acgucaucgg ggugcgccug      120

uucacccccg ccgagaccgc cgaggagcgg cgcggcuucc ggaccgcccg ccggcgccug      180

agccggcgcc gguggcgccu cggcgcccug aacgggaucu ucgcgccgga gcuggccaag      240

guggacccca acuuccucca ccggcugaag uacgccuggg uccaccccgc cgaccagcag      300

aacaagggca aguacuccgc ggcgccccug uucaacgacg ccgcccucga ccgccaguuc      360

cacgacgacu acccgacgau cuaccaccuc cggcugaagc ugaugaccga caccagccag      420

cacgaccugc gcgaggugua ccuggccauc caccacauca ucaaguuccg ggggaacuuc      480

cuccgcggcg aggggcccau caacgccucc gaggccuucg acaucaacac cuucaucgac      540

cagaugacgg ccuucuacga ccaggccuuc cccgacuuca agaucgugga ccaggucgac      600

cagcagauca ucuucaaggg ccugaccgac augacccuga gccccgccga ccgggcgaac      660

aaggcccuca ugggcuucaa caacgccgcc cagcagccga acaagcgcau cuucaaggcc      720

auccuguccg ggcugguggg caaccaggug gaccucaugg cgaucuucca gcuggacggg      780

auccccaagg aggaccagaa ggaguucaag uucaaguucg ucgacaacga caucgaggag      840

aagcuggccg cccucggcga caaccccgcc cugagcgacg agcagaacga guuccugacc      900

acgcucuacg ccgcguucuc cggccugacc cugaaggggc uccugggcga cgccaccagc      960

aucuccgccg ccaugguggc gcgguacgcc agccaccgca ccgacuggac guacaucaag     1020

gaccacgugc ugacgcccca gaacaaccgc gagcuccggc uggccuacgc cgaccugaug     1080

accgacaacg aggacguccg ccucaaggcc gggaagaagu ucgcgaaggc caucgaggcc     1140

gccgacaucc cggccgcgaa gaaguccgag uucagccuga agaucgacaa caacgaguuc     1200

cugccccggc agcgcaccaa ggccaacggc accauccccc accagcucca ccugaacgag     1260

cugcagcaga ucaucgagaa ccaggggcag uacuaccccu uccucaaggc caccuucgag     1320

caggacggcc acacccugaa caagaucgag ggccugguga aguuccgggu gccguacuac     1380

gucgggcccc ucgugcccaa gcacaaggug gccggcaaca acgccaacca cuggaugguc     1440

cccaaggagc cgggggcgau cacccccugg aacuacacgg agaaggugga ccgcgacaag     1500

uccggcaacg aguucauccg gcgcaugacc gggaccgaca ccuaccugcu gggcgagccc     1560

acgcugccca agaacagccu cguguaccag gaguucaacg uccugcagga gcugaacaac     1620

cuccggaucg agaagcgccg gcuggacauc gccgugaagc aggacaucuu cgagcacgug     1680

uucaagacca acaagaccgu caccgugaag cgcaucgagg aguaccugac gaccgaguuc     1740

ggcuccccgg ucacccucag cggguucgcc aacgugaacg agcggaagcu gaacuccagc     1800

cugagcaccu accacuacuu cgugggcauc uucggggccg aguucgucga cgaccccaag     1860

aaccucacgc ugcuggagca gaucguggag cuccagaccg uguucgagga ccgcaaggcg     1920

cugcagcggc agcugaagcu ccgccccgag cuguccgaga agcaggucaa gcagcuggcc     1980

cggacccacu acaccggcug ggggcgccuc agccagaagc ugcugaccuc caagaucgug     2040

cacguggccc ucgagggcga gcgggagccc ggccaguaca gcauccugga gacgcuguac     2100

gggacccaga ugaacuucau ggagaucguc aacgacauga aggaccgcua cggcgugaag     2160

uccuggcucg accaggagaa cagcgggucc gagaacaacc ugagccugaa gcagcgcauc     2220

cucgacaugc cgggccccaa gaacaucaag cggggcauca accaggccuu ccacaucauc     2280

gacgagauca cccgcgccgu ggggaacgcg cccucccgga ucuacaucga guucgcccgc     2340

gagagccagc aguccaaccu gaccaacgcc cgggucaacc gccugaagca gcucuacagc     2400

aaccaggccc ugaagaagga guucgccaag cuggcguccg agaucaagga ccucacgaag     2460

gagcagcuga aggacgaccg gcuguaccuc uacuaccugc agcagggcaa ggacauguac     2520

accgagaccg acaucgacau caccaagauc agcuccaacu accagaucga ccacaucauc     2580

ccgcaggccu acacgaagaa caacagccug gacaaccgcg ugcucgugaa cuccgagaug     2640

aacgcccgca agagcgacuc ccccaccuuc cugcccagcc ugauccagga gcggaaggcc     2700

ugguggcggc agcuccgcga caccgggcug augagcacca ucaaguacaa caaccugacg     2760

cggaccuccg acgacuucag cgccgaccag aaggagcgcu ucaucgcgcg gucccucguc     2820

gagacccgcc aggugaucaa gaacguggcc acccugauca aggaguacua cgacgacagc     2880

caggucgagg cccugcccuc cgagaucacg gccgagaugc ggcgcuaccu caacgugcgg     2940

aagaaccgcg acaucaacga cuaccaccac gcgcacgacg cccugcuguu cgccaccgcc     3000

ggcgaguuca gccggaaggu ggggaucaug gagaagggcc gcgucuccga cggcgccggg     3060

aacaucuaca accgcuacac caaggacauc auccagcggg cgcgcgccaa ggccggccag     3120

gacaagaagg ugcggccguu caccuucauc guggggagca uggccaaccc cgacacgaag     3180

uuccagacca accagaccac cggcgagauc gucuggacgc ccgaggacgu ggccuaccuc     3240

cgcaagguga ucgacuacaa gaagaugcug gucaccaaga ugacccugga ccagaccggg     3300

acgcucuaca acgagacccg guacggcucc ggcgacaagc acguggcggu ggcccugagc     3360

aaggacaagg ccgccgaccu guacgggggc uucggguccc ucaagcccgc cuacgcggcc     3420

cuggucuaca gcaagaagaa guuccgccug gugaacgugc uccggcugug gcugccggag     3480

aucaagaaga acgccuccgu ccucgagcgc aaggugcaga gcgugcuggg cgacgacaac     3540

gccaagguca uccgggccca cgugcccugc gggcagcugc ucaucaagga cggcgcgcug     3600

gugaccgucu ccagcgccac cgagcugcac aacuuccgcc agcucuggcu gcccaaggcc     3660

gacuaccagg acaucuccau ccugcucggc gcccccgaca aggugacggc ggagaaccgg     3720

cugcaggccg agagccacuu cgacgacgug gaccagcgcu ucgagagccu gcucaaggac     3780

gucaucgacc aggcccggca guacuacccg cugaagaccc accagaccuc ccuggagaag     3840

cucaucggga agaucggcaa guucgacgag cugaaguucg cccagaagca gcaggucuuc     3900

agccgccugc ucuccgcccu gcacgcgaac gccaccaaca gcgaccugaa gcagaucggg     3960

cucuccacgg ccuggggccg gcugaucuac cccaaggggg ugagccugac cgacgaggac     4020

aagcucauca acuccagcuu caccggccug uucaacgacg ugauguccau caaggaguuc     4080

cacgacuuca gcaucaacug a                                               4101


<210>  1935
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1935
augaagaagc agaaguucag cgacuacuac cugggcuucg acaucgggac caacuccgug       60

ggcuggugca ucaccgaccu cgacuacaac guccugcgcu ucaacaagaa ggacaugugg      120

gggagccggc uguucgagga ggccaagacg gccgcggagc gccgggugca gcgcaacucc      180

cggcgccggc ucaagcgccg gaaguggcgc cugaaccugc ucgaggagau cuucagcaac      240

gagauccuga agaucgacuc caacuucuuc cggcgccuga aggagagcuc ccucuggcug      300

gaggacaaga gcuccaagga gaaguucacc cuguucaacg acgacaacua caaggacuac      360

gacuucuaca agcaguaccc caccaucuuc caccuccgga acgagcugau caagaacccg      420

gagaagaagg acauccgccu gguguaccuc gccauccaca gcaucuucaa gucccggggc      480

cacuuccugu ucgaggggca gaaccugaag gagaucaaga acuucgagac ccucuacaac      540

aaccugaucg ccuuccugga ggacaacggc aucaacaaga ucaucgacaa gaacaacauc      600

gagaagcucg agaagaucgu cugcgacagc aagaagggcc ugaaggacaa ggagaaggag      660

uucaaggaga ucuucaacuc cgacaagcag cugguggcca ucuucaagcu cagcgugggg      720

uccagcgucu cccugaacga ccuguucgac acggacgagu acaagaaggg cgagguggag      780

aaggagaaga ucagcuuccg cgagcagauc uacgaggacg acaagcccau cuacuacucc      840

auccucgggg agaagaucga gcugcuggac aucgccaaga gcuucuacga cuucauggug      900

cucaacaaca uccuggcgga cagccaguac aucuccgagg ccaaggucaa gcuguacgag      960

gagcacaaga aggaccucaa gaaccugaag uacaucaucc ggaaguacaa caagggcaac     1020

uacgacaagc uguucaagga caagaacgag aacaacuaca gcgccuacau cgggcucaac     1080

aaggagaagg gcaagaagga ggugguggag aagucccgcc ugaagaucga cgaccugauc     1140

aaggucauca agggcuaccu ccccaagccc gagcggaucg aggagaagga caagaccgug     1200

uucaacgaga uccugagcaa gaucgagcug aagaccaucc ucccgaagca gcgcaucucc     1260

gacaacggga cccugcccua ccagauccac gagguggagc uggagaagau ccucgagaac     1320

cagagcaagu acuacgacuu ccugaacuac gaggagaacg gcgucuccac gaaggacaag     1380

cugcucaaga ccuucaaguu ccggaucccc uacuacgugg ggccccugaa cagcuaccac     1440

aaggacaagg gcggcaacuc cuggaucgug cgcaaggagg aggggaagau ccugccgugg     1500

aacuucgagc agaaggucga caucgagaag uccgccgagg aguucaucaa gcgcaugacc     1560

aacaagugca ccuaccugaa cggcgaggac gugaucccca aggacacguu ccucuacagc     1620

gaguacguga uccugaacga gcugaacaag guccagguga acgacgaguu ccucaacgag     1680

gagaacaagc ggaagaucau cgacgagcug uucaaggaga acaagaaggu gagcgagaag     1740

aaguucaagg aguaccugcu cgucaagcag aucguggacg ggaccaucga gcugaagggc     1800

gucaaggacu ccuucaacag caacuacauc uccuacaucc gcuucaagga caucuucggg     1860

gagaagcuga accucgacau cuacaaggag aucagcgaga aguccauccu guggaagugc     1920

cuguacggcg acgacaagaa gaucuucgag aagaagauca agaacgagua cggcgacauc     1980

cucaccaagg acgagaucaa gaagaucaac accuucaagu ucaacaacug ggggcggcug     2040

agcgagaagc ugcucacggg caucgaguuc aucaaccugg agaccgggga gugcuacucc     2100

agcgugaugg acgcgcugcg ccggaccaac uacaaccuca uggagcugcu gagcuccaag     2160

uucacccucc aggagagcau caacaacgag aacaaggaga ugaacgaggc cuccuaccgc     2220

gaccugaucg aggagagcua cguguccccc agccugaagc gggccaucuu ccagacgcuc     2280

aagaucuacg aggagauccg caagaucacc ggccgggucc ccaagaaggu guucaucgag     2340

auggcccgcg ggggcgacga guccaugaag aacaagaaga ucccggcccg gcaggagcag     2400

cugaagaagc uguacgacag cugcggcaac gacaucgcga acuucuccau cgacaucaag     2460

gagaugaaga acagccucau cuccuacgac aacaacagcc ugcgccagaa gaagcuguac     2520

cucuacuacc ugcaguucgg gaagugcaug uacaccggcc gggagaucga ccuggaccgc     2580

cuccugcaga acaacgacac cuacgacauc gaccacaucu acccccgguc caaggugauc     2640

aaggacgaca gcuucgacaa ccugguccuc gugcugaaga acgagaacgc cgagaagucc     2700

aacgaguacc ccgugaagaa ggagauccag gagaagauga aguccuucug gcgcuuccug     2760

aaggagaaga acuucaucag cgacgagaag uacaagcggc ucacggggaa ggacgacuuc     2820

gagcugcgcg gcuucauggc ccggcagcug gucaacgugc gccagaccac caaggaggug     2880

gggaagaucc uccagcagau cgagcccgag aucaagaucg ucuacagcaa ggccgagauc     2940

gccagcuccu uccgcgagau guucgacuuc aucaaggugc gggagcugaa cgacacccac     3000

cacgcgaagg acgccuaccu gaacaucgug gccggcaacg ucuacaacac gaaguucacc     3060

gagaagccgu accgcuaccu ccaggagauc aaggagaacu acgacgugaa gaagaucuac     3120

aacuacgaca ucaagaacgc cugggacaag gagaacagcc uggagaucgu gaagaagaac     3180

auggagaaga acaccgucaa caucacccgg uucaucaagg aggagaaggg ccagcuguuc     3240

gaccucaacc ccaucaagaa gggggagacg uccaacgaga ucaucagcau caagcccaag     3300

guguacaacg gcaaggacga caagcugaac gagaaguacg gguacuacaa gucccugaac     3360

cccgccuacu uccucuacgu ggagcacaag gagaagaaca agcgcaucaa gagcuucgag     3420

cgggucaacc ugguggacgu gaacaacauc aaggacgaga agucccuggu caaguaccuc     3480

aucgagaaca agaagcuggu ggagccgcgc gugaucaaga aggucuacaa gcgccaggug     3540

auccugauca accacuaccc cuacagcauc gugacccucg acuccaacaa gcugauggac     3600

uucgagaacc ugaagccccu cuuccuggag aacaaguacg agaagauccu gaagaacguc     3660

aucaaguucc ucgaggacaa ccagggcaag agcgaggaga acuacaaguu caucuaccug     3720

aagaagaagg accgguacga gaagaacgag acccuggagu ccgugaagga ccgguacaac     3780

cucgaguuca acgagaugua cgacaaguuc cuggagaagc uggacagcaa ggacuacaag     3840

aacuacauga acaacaagaa guaccaggag cuccuggacg ugaaggagaa guucaucaag     3900

cugaaccucu ucgacaaggc guucacccug aaguccuucc uggaccucuu caaccgcaag     3960

acgauggccg acuucagcaa ggucggccug accaaguacc uggggaagau ccagaagauc     4020

uccagcaacg ugcucuccaa gaacgagcug uaccugcucg aggagagcgu gaccggccug     4080

uucgucaaga agaucaagcu guga                                            4104


<210>  1936
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1936
augaagaagc agaaguucag cgacuacuac cugggcuucg acaucgggac caacuccgug       60

ggcuggugcg ucaccgaccu cgacuacaac gugcugcgcu ucaacaagaa ggacaugugg      120

gggagccggc uguucgacga ggccaagacg gccgcggagc gccgggugca gcgcaacucc      180

cggcgccggc ucaagcgccg gaaguggcgc cugaaccugc ucgaggagau cuucagcgac      240

gagaucauga agaucgacuc caacuucuuc cggcgccuga aggagagcuc ccuguggcuc      300

gaggacaaga acagcaagga gaaguucacc cuguucaacg acgacaacua caaggacuac      360

gacuucuaca agcaguaccc caccaucuuc caccuccggg acgagcucau caagaacccg      420

gagaagaagg acauccgccu gaucuaccug gcccuccacu ccaucuucaa gagccggggc      480

cacuuccugu ucgaggggca gaaccugaag gagaucaaga acuucgagac ccucuacaac      540

aaccugaucu ccuuccugga ggacaacggc aucaacaaga gcaucgacaa ggacaacauc      600

aagaagcucg agaagaucau cugcgacucc ggcaaggggc ugaaggacaa ggagaaggag      660

uucaaggaga ucuucaacag cgacaagcag cuggucgcca ucuucaagcu cuccgugggc      720

agcuccguga gccugaacga ccuguucgac acggacgagu acaagaagga ggaggucgag      780

aaggagaaga ucuccuuccg cgagcagauc uacgaggacg acaagcccau cuacuacagc      840

auccucgggg agaagaucga gcugcuggac aucgccaaga gcuucuacga cuucauggug      900

cucaacaaca uccuguccga cagcaacuac aucuccgagg ccaaggugaa gcuguacgag      960

gagcacaaga aggaccucaa gaaccugaag uacaucaucc ggaaguacaa caaggagaac     1020

uacgacaagc uguucaagga caagaacgag aacaacuacc ccgcguacau cggccucaac     1080

aaggagaagg ggaagaagga ggucguggag aaguccggcc ugaagaucga cgaccugauc     1140

aaggugauca agggcuaccu ccccaagccg gagcgcaucg aggagaagga caagaccauc     1200

uucaacgaga uccugaacaa gaucgagcug aagaccaucc uccccaagca gcggaucagc     1260

gacaacggga cccugcccua ccagauccac gaggucgagc uggagaagau ccucgagaac     1320

cagagcaagu acuacgacuu ccugaacuac gaggagaacg gcguguccac gaaggacaag     1380

cugcucaaga ccuucaaguu ccgcaucccc uacuacgugg ggccgcugaa cagcuaccac     1440

aaggacaagg gcggcaacuc cuggaucguc cggaaggagg aggggaagau ccugcccugg     1500

aacuucgagc agaaggugga caucgagaag uccgccgagg aguucaucaa gcgcaugacc     1560

aacaagugca ccuaccugaa cggcgaggac gugaucccca aggacagcuu ccucuacagc     1620

gaguacauca uccugaacga gcugaacaag guccagguga acgacgaguu ccucaacgag     1680

gagaacaagc gcaagaucau cgacgagcug uucaaggaga acaagaaggu guccgagaag     1740

aaguucaagg aguaccugcu cgucaaccag aucgccaacc ggacggugga gcugaagggg     1800

aucaaggaca gcuucaacuc caacuacgug agcuacauca aguucaagga caucuucggc     1860

gagaagcuga accucgacau cuacaaggag aucuccgaga aguccauccu guggaagugc     1920

cuguacgggg acgacaagaa gaucuucgag aagaagauca agaacgagua cggcgacauc     1980

cucaacaagg acgagaucaa gaagaucaac agcuucaagu ucaacaccug gggccgccug     2040

agcgagaagc ugcucaccgg gaucgaguuc aucaaccugg agaccggcga gugcuacagc     2100

uccgucaugg aggcccugcg gcgcacgaac uacaaccuca uggagcugcu gagcuccaag     2160

uucacccucc aggagagcau cgacaacggg aacaaggaga ugaacgaggu guccuaccgg     2220

gaccugaucg aggagagcua cgucuccccc agccugaagc gcgcgauccu ccagacccug     2280

aagaucuacg aggagaucaa gaagaucacc ggccgggugc cgaagaaggu guucaucgag     2340

auggcccgcg ggggcgacga gacgaugaag aacaagaaga uccccgcccg gcaggagcag     2400

cugaagaagc ucuacgacuc cugcggcaac gacaucgcca acuucagcau cgacaucaag     2460

aagaugaagu ccagccuguc cagcuacgac aacaacuccc ugcgccagaa gaagcucuac     2520

cuguacuacc ugcaguucgg gaagugcaug uacaccggcc gggagaucga ccucgaccgc     2580

cugcugcaga acaacgacac cuacgacauc gaccacaucu acccccggag caaggucauc     2640

aaggacgacu ccuucgacaa ccucgugcug gugcugaaga acgagaacgc cgagaagucc     2700

aacgaguacc ccgucaagaa ggagauccag gagaagauga aguccuucug gcgcuuccuc     2760

aaggagaaga acuucaucag cgacgagaag uacaagcggc ugaccgggaa ggacgacuuc     2820

gagcugcgcg gcuucauggc gcggcagcuc gugaacgugc gccagacgac caaggagguc     2880

gggaagaucc ugcagcagau cgagccggag aucaagaucg uguacagcaa ggccgagauc     2940

gccagcagcu uccgggagau guucgacuuc aucaaggugc gcgagcugaa cgacacccac     3000

cacgccaagg acgccuaccu caacaucguc gcgggcaacg uguacaacac caaguucacg     3060

gagaagcccu accgcuaccu gcaggagauc aaggagaacu acgacgugaa gaagaucuac     3120

aacuacgaca ucaagaacgc cugggacaag gagaacuccc uggagaucgu caagaagaac     3180

augaagaaga acaccgugaa caucacccgg uucaucaagg aggagaaggg ccagcuguuc     3240

gaccugaacc ccaucaagaa gggggagacc agcaacgaga ucaucuccau caagcccaag     3300

cuguacaacg gcaaggagga gaagcucaac gagaaguacg gguacuacaa gagccugaac     3360

ccggccuacu uccuguacgu ggagcacaag gagaagaaca agcgcaucaa guccuucgag     3420

cgggucaacc ucguggacgu gaacaacauc aaggacgaga agucccuggu caaguaccug     3480

aucgagaaca agaagcucgu ggagccccgc cugaucaaga agguguacaa gcggcagguc     3540

auccugauca acgacuaccc cuacagcauc guggcccucg acagcaacaa gcugauggac     3600

uucgagaacc ugaagccccu cuuccuggag aacaaguacg agaagauccu gaagaacgug     3660

aucaaguucc ucgaggacaa ccagggcaag uccgaggaga acuacaaguu caucuaccug     3720

aagaagaagg accgcuacga gaagaacgag acgcuggaga gcgucaagga ccgguacaac     3780

cucgaguuca acgagaugua cgacaaguuc cuggagaagc uggacuccaa ggacuacaag     3840

aacuacauca acaacaagaa guacaagaac cuccuggacg ugaaggagaa guucaucaag     3900

cugaaccucu ucgacaaggc cuucacccuc aagagcuucc uggaccuguu caaccgcaag     3960

accauggcgg acuucuccaa ggugggccug accaaguacc uggggaagau ccagaagauc     4020

agcuccaacg uccucagcaa gaacgagcug uaccugcucg aggagagcgu gacgggccug     4080

uucgugaaga agaucaagcu guga                                            4104


<210>  1937
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1937
augaagaagc agcaguucag cgacuacuac cugggcuucg acaucgggac caacuccgug       60

ggcuggugcg ucaccgacuu caacuacaac gugcuccgcu ucaacaagaa ggacaugugg      120

gggagccggc uguucgacga ggccaagacg gccgcggagc gccgggugca gcgcaacucc      180

cggcgccggc ugaagcgccg gaaguggcgc cucaaccugc uggaggagau cuucagcgac      240

gagauccuca agaucgacuc caacuucuuc cggcgccuga aggagagcuc ccuguggcuc      300

gaggacaaga gcuccaagga gaaguucacc cuguucaacg acgacaacua caaggacuac      360

gacuucuaca agcaguaccc caccaucuuc caccuccgga acgagcugau caagaacccg      420

gagaagaagg acauccgccu ggucuaccug gcccuccaca gcaucuucaa gucccggggc      480

cacuuccugu ucgaggggca gaaccugaag gacaucaaga acuucgagac ccucuacaac      540

aaccugaugg ccuuccugga ggacaacgac aucuacaaga acaucgacag cuccuacauc      600

ggcaaccucg agaacaucau cugcgacagc aagaagggcc ugaaggacaa ggagaaggag      660

uucaaggaga ucuucaacuc cgacaagcag cugguggggu ucuucaagcu cagcgugggc      720

uccagcgucu cccugaacga ccuguucgac acggacgagu acaagaaggg ggagguggag      780

aaggagaaga ucagcuuccg cgagcagauc uacgaggacg acaagcccau cuacuacagc      840

auccucggcg agaagaucga guuccuggac aucgccaagu ccuucuacga cuucauggug      900

cugaacaaca uccucgccga cagccaguac aucuccgagg cgaaggucaa gcuguacgac      960

gagcacaagc gggaccugaa gaaccucaag uacaucaucc gcaaguacaa caaggagaac     1020

uacgacaagc uguucaagga caagaacgag agcaacuacu ccgccuacau cgggcugaac     1080

aaggagaagg gcaagaagga ggugaucgag aagucccggc ucaagaucga cgacuucgcc     1140

aagaucauca agggcuaccu gcccaaggcc gagaagaucg acgagaagga ccgcagcauc     1200

uucaacgaga uccuggacaa gaucgagcuc aagaccaucc ugcccaagca gcggaucagc     1260

gacaacggga cccugccgua ccagauccac gaggcggagc ucgagaagau ccuggagaac     1320

caggccaagu acuacgacuu ccugaaccac gaggagaacg gcaucuccac caaggacaag     1380

cuccugauga cguucaaguu ccgcaucccc uacuacgugg ggccccugaa cagcuaccac     1440

aagaacaagg gcggcaacuc cuggaucguc cgcaaggagg aggggaagau ccuccccugg     1500

aacuucgagc agaaggugga caucgagaag uccgccgagg aguucaucaa gcggaugacc     1560

aacaagugca ccuaccugaa cggcgaggac gugaucccga aggacagcuu ccuguacagc     1620

gaguacauca uccugaacga gcucaacaag guccagguga acgacgaguu ccugaacaag     1680

gagaucaaga agaagaucau cgaggaccug uucaagaagu ccaagaagau cagcgagaag     1740

aacuuccgcg aguaccuccu ggugaaccag aucaccaaca agacggucga gcugaagggg     1800

gugaaggacg ccuucaacuc caacuacgug agcuacauca aguucaagga caucuucggc     1860

gacaagcuca accuggacau cuacaaggag aucuccgaga aguccauccu guggaagugc     1920

cucuacgggg acgacaagaa gaucuucgag aagaagauca agagcgucua cggcgacauc     1980

cugaccaagg acgagaucaa gaagaucaac agcuucaagu ucaacaccug gggccggcug     2040

agcgagaagc uccugaccga gaucgaguuc aucgaccugg agacggggga gugcuacucc     2100

agcgugaugg acgcccuccg ccggaccaac uacaaccuga uggagcugcu cuccagcaag     2160

uucacccugc aggagaacau cgacaacgag aacaaggagg uguccgaguu cagcuaccgc     2220

gaccuggucg aggaguccua cgugagcccc ucccucaagc gggcgauccu gcagacccug     2280

aagaucuacg aggagauccg caagaucacg ggccggaucc ccaagaaggu cuucaucgag     2340

auggcccgcg ggggcgacga gaccaugaag aacaagaaga uccccgcccg gcaggagcag     2400

cucaagaagc uguacgacag cugcgggaag gacaucucca acuucagcau cgacaucaag     2460

gagaugaaga acucccugaa cagcuacgac aacaacuccc uccgccagaa gaagcuguac     2520

cuguacuacc uccaguucgg caagugcaug uacaccggca aggagaucga ccugaaccgg     2580

cugcuccaga acaacgacac cuacgacauc gaccacaucu acccgcgcag caaggugauc     2640

aaggacgaca gcuucgacaa ccuggugcug guccucaaga acgagaacgc cgagaagucc     2700

aacgaguacc cccugaagaa ggagauccag gagaagauga aguccuucug gaaguuccug     2760

aaggagaaga acuucaucag cgacgagaag uacaagcggc ucacggggaa ggacgaguuc     2820

gagcugcgcg gcuucauggc ccggcagcug gugaacgugc gccagaccac caaggaggcg     2880

gggaagaucc uccagcagau cgagcccgag aucaagaucg ucuacagcaa ggccgagauc     2940

gccuccagcu uccgggagau guucgacuuc aucaaggugc gcgagcugaa cgacacccac     3000

cacgccaagg acgccuaccu gaacaucgug gcgggcaacg ucuacaacac gaaguucacc     3060

gagaagcccu accgcuaccu ccaggagauc aaggagaacu acgacgugaa gaagaucuac     3120

aacuacgaca ucaagaacgc cugggacaag gagaaguccc uggagaucgu gaagaagaac     3180

auggagaaga acaccgucaa caucacccgg uucaucaagg aggagaaggg gcagcuguuc     3240

gaccucaacc cgauccgcaa gggcgagacg agcaacgaga ucaucgccau caagcccaag     3300

uucuacgagg gcuccaccga gaagcugaac gagaaguacg gguacuacaa gagccugaac     3360

cccgccuacu ucaucuacgu ggagcacaag gagaagaaca agaucauccg guccuucgag     3420

cgcgugaacc ucgucgacgu gaacaagauc aaggacgaga agucccugau caaguaccug     3480

aucgagaaca agggccucau cgagcccaag gugaucaaga aggucuacaa gcgccaggug     3540

auccugauca acaacuuccc guacagcauc guggcccugg acagcaacaa gcucauggac     3600

uucgagaacc ugaagccccu guuccucgag cggaaguacg agaagauccu gaagaacgcg     3660

aucaaguucc uggaggacaa ccaggggaag accgaggagu acuacaaguu ccucuaccug     3720

aagaagaagg accggaacga gaagaacgag accaucgacu ccgucaagga gcgcuacaac     3780

aucgaguuca acgagaugua cgacaaguuc cuggagaagc ucgacagcaa ggacuacaag     3840

aacuacauca acaacaagaa guacuccgag cuggugaacg ugaaggagaa guucaucaag     3900

cugaaccucu ucgacaaggc cuucacgcuc aagagcuucc uggaccuguu caaccggaag     3960

accauggccg acuucuccaa ggucggccug accaaguacc ugggcaagau ccagaagauc     4020

agcagcaacg ugcucuccaa gaacgagcug caccuccugg aggagagcgu gaccgggcug     4080

uucgucaaga agaucaagcu guga                                            4104


<210>  1938
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1938
augaaggacu acuaccuggg ccucgacaug gggagcucca gcgugggcug ggcccugucc       60

gacgagcgcu acaaccugcu ccgggccaag gggaaggacc uguggggcgu ccgccuguuc      120

gacgaggcga agaccgccgc cgagcggcgc accuuccgga gccagcgccg gcgccucgag      180

cgguccaagu ggcgccugag ccugcucacg ucccuguucg aggaggagau cagcaagauc      240

gacgacaagu ucuucgaccg gcugaaggac uccaaguucu ucaucgacga caagagcgac      300

cagcagccca acacccucuu caacgacgug aacuucaccg acaaggagua ccacaagcgc      360

uacccgucca ucuaccaccu ccggaacgcc cuguucaacc agaacgacaa guucgacauc      420

cgccucaucu uccuggcccu gagccacauc gugaagaagc gggggcacuu ccucuucgac      480

ggcgacaagg cgggcuucgg ggaggacauc gacgucaucc uggacgagcu gaaggccuac      540

cuccaggacg ccuuccacac caagcuggag uucacguccg aguucaagag caucaugcug      600

gacaagaacc uccgcaagaa gcagaaggag gaggagcuga agaacuccau caagaucagc      660

uccgacacca ucaacagcaa ggacggcaag uccauccuga ucaagcuguu ccugggguac      720

gaggccaacc ugaagcccuu cuuccucgag uacaaggugg agaaggacaa cgaguacaag      780

aucaacuuca gcaaggagga cuacgaggag aagcugcugg acaugaccag caaguucgag      840

gagaccacgu acuucaucga gaucgcgaag aagauccacg acuacuuccu ccuggaggag      900

aucaugcugg gcuucaagaa caucuccgcc agcaaggugu ccaucuacca gaagcacaag      960

aacgaccuca agacccugaa gaacaucguc aagaaccaga ucaacaacag cuacaacgcc     1020

aucuucaagg acaucgggcu ggacaacuac cccgccuacg uguccugcug gaacagcaag     1080

aagcacaagg agaagcagaa gggcugcacc aaggacaagu ucuacgccuu ccucaagacc     1140

aagcugaaga acaucaacaa cgcgaaggag auccuggacu ccaucgacaa cgacgacuuc     1200

cucccccggc ugguggacaa gaacagcauc aucccgcacc agauccaccu ggccgagcuc     1260

aucaagauca ucgacaacca gucccaguac uaccccuucc ugaaggagau caaggacaag     1320

aucaucagca ucuucaaguu ccgcaucccc uucuacgucg gcccccugaa caacuuccac     1380

aaggacaagg gcgggaacgc cuggaucgag aagcucaagg acgagcggau cuucccgugg     1440

aacuucaacg acguggugga ccugaacaag uccgccgcca acuucaucga gaagaugauc     1500

aacgagugca gcuaccugaa gggggagaag guccuggcga agaacucccu ccuguacacg     1560

aaguacagcg ugcugaaccu caucaacaag cugaagauca acggcaccgc cguguccguc     1620

gagaccaagc agaagaucua caacgagcug cuccugaagc agaagaacau caagaagacc     1680

gccaucaaga aguuccugau caacaacagc aucaucgaca agaacgacga gaucuccggc     1740

aucgacgaca gccucacguc caaccuggcc guguucaucg acuucgagaa gaucagcaac     1800

cgccugcggu acgaccagaa ggaggacauc aucaagagca ucucccucuu cggguacgac     1860

aacgccaugc ugaagaagca gaucaucacc aucgcgcccg accugagcga ggacgagcuc     1920

gagaagcugu ccaagcugcg cuacagcggc uucgggcgcc ucuccaagcg gcugcugacc     1980

agcaucaagc acaacggcaa guccaucauc gagaugcucu gggacaccaa cgacaaccug     2040

auggagcugc ucagcgaccg cuacacguuc aucgagcaga ucaacaagca caacgacggg     2100

uucaaggagg acaucaaccu gaacuuccag aacguggaca cccuguacgu cucccccagc     2160

gugaagcggc agaucuggca ggccuucaag gugaucaacg agcucaucuc cgucaugggc     2220

cgcgagccca agaagaucuu cguggagaug gcccgggagg agggcgagaa gaagcgcacc     2280

caggcccgga aggacaaggu gcuggagcug uacaagagca ucaagaacaa cgagaucucc     2340

accuuccuca cgaacuucaa ggacaucaag accaagcugg agggggagag caaccugaac     2400

uccaagaagc ucuaccugua cuaccugcag cucggcgucg acaucuacac cggggagaag     2460

aucccguucg agaagcuguu cgaccagaac aucuacgaca ucgaccacgu gaucccccgc     2520

agccugaaga aggacgacuc cuucgacaac cucguccuga ccagcaagac gaacaacgcc     2580

aagaagaccg acgaguuccc cgugagcucc gagauccaga gcaagaugca gaccuucugg     2640

aaggugcuga aggacaagga ccucaugucc gagaucaagu acaccaaccu gacgcggaag     2700

acccaccuga gcgacgacga gcucggcgac uucaucgcgc gccagcuggu cgagacccgg     2760

caguccacca aggcccugac gcagauccuc aagaacgugu acgccaacag cgacaucgug     2820

uacgucaagg ccgggaucgu guccgaguuc cgccagcagu ucgacaugcu gaagauccgg     2880

gagcugaacg acuuccacca cgcgaacgac gccuaccuca acaucgugau cggcaacguc     2940

uacaacauca aguucaccaa gaacccccgc aacuacguga aggaguacua caagaagggc     3000

gagcacuaca accugaagau cgagaagaug cuggagaagg acaucgcccg cgggggcaac     3060

uucggguggg acaagaagca cagcaucgag accgugcaga agaccaugaa gaagcagucc     3120

ccgcucauca ccuaccggac guucgagaac aagggcgagu ucuucaacca gcagcccagc     3180

aaggccuccg ccaaccugcu ggcgcucaag agcuccgaca agcggcugcu ggacaccguc     3240

cgcuacgggg gcuacaaggg cacccaguac gcccacaugu uccucgugga gcacuucaac     3300

gggaagauca ucaaggagau caugggcgug cccaucuacc ugagcaagga gaucaaggac     3360

gacaaggcca agcugcucga guacugcaac gacguccuga agcugaagaa ccccaagauc     3420

cucuucucca agaucccgua caacagccug aucguguaca acggguaccc cauccugcuc     3480

cggggccuga ccggcgugcg cggggccgcc cagcugaagc ucgaccagac guccacccag     3540

gucgugaaga acaucgugaa gcuguacgac uggcugagcc agaagcagaa ggagcucucc     3600

gaccugagca agaagaucga guucuuccag gagaacaaca agaaggacga ggucaaggcg     3660

gccaugcugg aggccgacaa cauccucuuc auccugaacg accgcuucaa gaacaccaag     3720

aucggcaagg acaucaugaa cgacaaccac cugaacaacc ucuacgacac cuucaucaac     3780

aagcuggaga acagcaucua caagaacaag cccgggggca agcaggacgu gcugaugaag     3840

gagaagcggg accucuuccu guccuucagc auguccaaga agugcaucac gcuguacaag     3900

aucaucaaga ucuucgggag cugcgaccag ggcguggacu ucuccgacau cgccgagaag     3960

aucagcaagu ccggcgggau cggcgggaag aaccucggca agaacaccuu cgaccugguc     4020

uucaaggaga acagcaccua cacccuggug caguccagcg ugacggggcu cuacgccaag     4080

aagaccgacc ugcacaagcu guga                                            4104


<210>  1939
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1939
augaagaagc agaaguucag cgacuacuac cugggcuucg acaucgggac caacuccgug       60

ggcuggugcg ucaccgaccu cgacuacaac gugcugcgcu ucaacaagaa ggacaugugg      120

gggagccggc uguucgagga ggccaagacg gccgcggagc gccgggugca gcgcaacucc      180

cggcgccggc ucaagcgccg gaaguggcgc cugaaccugc ucgaggagau cuucagcaac      240

gagauccuga agaucgacuc caacuucuuc cggcgccuga aggagagcuc ccucuggcug      300

gaggacaaga gcuccaagga gaaguucacc cuguucaacg acgacaacua caaggacuac      360

gacuucuaca agcaguaccc caccaucuuc caccuccgga acgagcugau caagaacccg      420

gagaagaagg acauccgccu ggucuaccuc gccauccaca gcaucuucaa gucccggggc      480

cacuuccugu ucgaggggca gaaccugaag gagaucaaga acuucgagac ccucuacaac      540

aaccugaucg ccuuccugga ggacaacggc aucaacaaga ucaucgacaa gaacaacauc      600

gagaagcucg agaagaucgu gugcgacagc aagaagggcc ugaaggacaa ggagaaggag      660

uucaaggaga ucuucaacuc cgacaagcag cugguggcca ucuucaagcu cagcgucggg      720

uccagcgugu cccugaacga ccuguucgac acggacgagu acaagaaggg cgagguggag      780

aaggagaaga ucagcuuccg cgagcagauc uacgaggacg acaagcccau cuacuacucc      840

auccucgggg agaagaucga gcugcuggac aucgccaaga ccuucuacga cuucaugguc      900

cucaacaaca uccuggcgga cagccaguac aucagcgagg ccaaggugaa gcuguacgag      960

gagcacaaga aggaccucaa gaaccugaag uacaucaucc ggaaguacaa caagggcaac     1020

uacgacaagc uguucaagga caagaacgag aacaacuacu ccgccuacau cgggcucaac     1080

aaggagaagu ccaagaagga ggugaucgag aagucccgcc ugaagaucga cgaccugauc     1140

aagaacauca agggcuaccu ccccaagguc gaggagaucg aggagaagga caaggccauc     1200

uucaacaaga uccugaacaa gaucgagcug aagaccaucc uccccaagca gcggaucagc     1260

gacaacggca cccugccgua ccagauccac gaggcggagc uggagaagau ccucgagaac     1320

cagagcaagu acuacgacuu ccugaacuac gaggagaacg ggaucaucac gaaggacaag     1380

cugcucauga ccuucaaguu ccgcaucccc uacuacgugg gcccccugaa cagcuaccac     1440

aaggacaagg gcgggaacuc cuggaucgug cggaaggagg agggcaagau ccugcccugg     1500

aacuucgagc agaaggucga caucgagaag uccgccgagg aguucaucaa gcgcaugacc     1560

aacaagugca ccuaccugaa cggggaggac gugaucccga aggacacguu ccucuacagc     1620

gaguacguga uccugaacga gcugaacaag guccagguga acgacgaguu ccucaacgag     1680

gagaacaagc gcaagaucau cgacgagcug uucaaggaga acaagaaggu gagcgagaag     1740

aaguucaagg aguaccugcu cgucaagcag aucguggacg gcaccaucga gcugaagggg     1800

gugaaggacu ccuucaacag caacuacauc uccuacaucc gguucaagga caucuucggc     1860

gagaagcuga accucgacau cuacaaggag aucagcgaga aguccauccu guggaagugc     1920

cuguacgggg acgacaagaa gaucuucgag aagaagauca agaacgagua cggcgacauc     1980

cucaccaagg acgagaucaa gaagaucaac accuucaagu ucaacaacug gggccgccug     2040

agcgagaagc ugcucacggg gaucgaguuc aucaaccugg agaccggcga gugcuacucc     2100

agcgucaugg acgcccugcg gcgcaccaac uacaaccuca uggagcugcu guccagcaag     2160

uucacccucc aggagagcau caacaacgag aacaaggaga ugaacgaggc cuccuaccgg     2220

gaccugaucg aggagagcua cguguccccc agccugaagc gcgccaucuu ccagacgcuc     2280

aagaucuacg aggagauccg gaagaucacc gggcgcgucc ccaagaaggu guucaucgag     2340

auggcgcggg gcggggacga guccaugaag aacaagaaga uccccgcccg ccaggagcag     2400

cugaagaagc uguacgacag cugcggcaac gacaucgcca acuucuccau cgacaucaag     2460

gagaugaaga acagccucau cuccuacgac aacaacagcc ugcggcagaa gaagcuguac     2520

cucuacuacc ugcaguucgg caagugcaug uacaccgggc gcgagaucga ccuggaccgg     2580

cuccugcaga acaacgacac cuacgacauc gaccacaucu acccgcgcuc caaggugauc     2640

aaggacgaca gcuucgacaa ccugguccuc gugcugaaga acgagaacgc cgagaagucc     2700

aacgaguacc ccgugaagaa ggagauccag gagaagauga aguccuucug gcgguuccug     2760

aaggagaaga acuucaucag cgacgagaag uacaagcgcc ucacgggcaa ggacgacuuc     2820

gagcugcggg gguucauggc ccgccagcug gucaacgugc ggcagaccac caaggaggug     2880

ggcaagaucc uccagcagau cgagcccgag aucaagaucg ucuacagcaa ggcggagauc     2940

gccuccagcu uccgcgagau guucgacuuc aucaaggugc gcgagcugaa cgacacccac     3000

cacgccaagg acgccuaccu gaacaucgug gccgggaacg ucuacaacac gaaguucacc     3060

gagaagcccu accgguaccu ccaggagauc aaggagaacu acgacgugaa gaagaucuac     3120

aacuacgaca ucaagaacgc gugggacaag gagaacagcc uggagaucgu gaagaagaac     3180

auggagaaga acaccgucaa caucacccgc uucaucaagg agaagaaggg ccagcuguuc     3240

gaccucaacc cgaucaagaa gggcgagacg uccaacgaga ucaucagcau caagcccaag     3300

guguacaacg ggaaggacga caagcugaac gagaaguacg gcuacuacaa gucccugaac     3360

cccgccuacu uccucuacgu ggagcacaag gagaagaaca agcggaucaa gagcuucgag     3420

cgcgucaacc ugguggacgu gaacaacauc aaggacgaga agucccuggu caaguaccuc     3480

aucgagaaca agaagcuggu ggagccccgg gugaucaaga aggucuacaa gcgccaggug     3540

auccugauca acgacuaccc guacagcauc gugacccucg acuccaacaa gcugauggac     3600

uucgagaacc ugaagccccu cuuccuggag aacaaguacg agaagauccu gaagaacguc     3660

aucaaguucc ucgaggacaa ccagggcaag agcgaggaga acuacaaguu caucuaccug     3720

aagaagaagg accgguacga gaagaacgag acccuggagu ccgugaagga ccgcuacaac     3780

cucgaguuca acgagaugua cgacaaguuc cuggagaagc uggacagcaa ggacuacaag     3840

aacuacauga acaacaagaa guaccaggag cuccuggacg ugaaggagaa guucaucaag     3900

cugaaccucu ucgacaaggc cuucacccug aaguccuucc uggaccucuu caaccggaag     3960

acgauggccg acuucagcaa ggucgggcug accaaguacc ugggcaagau ccagaagauc     4020

uccagcaacg ugcucuccaa gaacgagcug uaccugcucg aggagagcgu gaccgggcug     4080

uucgucaaga agaucaagcu guga                                            4104


<210>  1940
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1940
augaagaagc agaaguucag cgacuacuac cugggcuucg acaucgggac caacuccgug       60

ggcuggugcg ucaccgacau gaacuacaac gugcuccgcu ucaacaagaa ggacaugugg      120

gggagccggc uguucgagga ggccaagacg gccgcggagc gccgggugca gcgcaacucc      180

cggcgccggc ugaagcgccg gaaguggcgc cucaaccugc uggaggagau cuucagcaac      240

gagauccuca agaucgacuc caacuucuuc cggcgccuga aggagagcuc ccuguggcuc      300

gaggacaaga gcuccaagga gaaguucacc cuguucaacg acgacaacua caaggacuac      360

gacuucuaca agcaguaccc caccaucuuc caccuccgga acgagcucau caagaacccg      420

gagaagaagg acauccgccu ggucuaccug gccauccaca gcaucuucaa gucccggggc      480

cacuuccucu ucgaggggca gaaccugaag gagaucaaga acuucgagac ccuguacaac      540

aaccucaucg ccuuccugga ggacaacggc aucaacaaga gcaucgacaa ggacaaccug      600

gagaagcucg agaagaucgu gugcgacucc ggcaaggggc ugaaggacaa ggagaaggag      660

uucaaggaga ucuucaacag cgacaagcag cugguggcca ucuucaagcu cuccgucggc      720

cugagcgugu cccugaacga ccucuucgac acggacgagu acaagaaggg ggagguggag      780

aaggagaaga ucagcuuccg cgagcagauc uacgaggacg acaagcccau cuacuacucc      840

auccugggcg agaagaucga gcugcucgac aucgccaaga gcuucuacga cuucaugguc      900

cugaacaaca uccuggcgga cagccaguac aucuccgagg ccaaggugaa gcucuacgag      960

gagcacaagc gggaccugaa gaaccugaag uacaucaucc gcaaguacaa ccgggagaac     1020

uacgacaagc uguucaagga caagaacgag agcaacuacc ccgccuacau cgggcugaac     1080

aaggagaagu ccaagaagga ggugaucgag aaguccaagc ugaagaucga cgaccucauc     1140

aagagcauca agggcuaccu gcccaagguc gaggagaucg aggagaagga caaggccauc     1200

uucaacgaga uccugaacaa gaucgagcuc aagaucaucc ugccgaagca gcgcaucagc     1260

gacaacggca cccugcccua ccagauccac gaggcggagc ucgagaagau ccuggagaac     1320

caguccaagu acuacgacuu ccugaacuac gaggagaacg ggaucaucac caaggacaag     1380

cuccugauga ccuucaaguu ccggaucccc uacuacgugg gcccccugaa cagcuaccac     1440

aaggacaagg gcgggaacuc cuggaucgug cgcaaggagg agggcaagau ccugccgugg     1500

aacuucgagc agaaggucga caucgagaag uccgccgagg aguucaucaa gcgcaugacg     1560

aacaagugca ccuaccucaa cggggaggac gugaucccca aggacagcuu ccuguacagc     1620

gaguacauca uccugaacga gcucaacaag gugcagguca acgacgaguu ccugaacgag     1680

gagaacaagc ggaagaucau cgaggagcug uucaagggca acaagaaggu guccgagaag     1740

aaguucaagg aguaccuccu ggugaaccag aucaccaccg ggacgaucga gcugaagggc     1800

aucaaggaca gcuucaacuc caacuacauc agcuacauca aguucaagga caucuucggg     1860

gagaagcuca accuggacau cuacaaggag aucuccgaga aguccauccu guggaagugc     1920

cucuacggcg acgacaagaa gaucuucgag aagaagauca agaacgagua cggcgacauc     1980

cugaccaagg acgagaucaa gaagaucaac accuucaagu ucaacaacug ggggcgccug     2040

agcgagaagc uccugaccgg caucgaguuc aucaaccugg agacggggga gugcuacagc     2100

agcgucaugg aggcccuccg gcgcaccaac uacaaccuga uggagcugcu cuccagcaag     2160

uucacccugc aggagaacau cgacaacgag aacaaggaca ucucccaguu cagcuaccgg     2220

gaccuggugg aggaguccua cgugagcccc ucccucaagc gcgccauccu gcagacccug     2280

aagaucuacg aggagaucaa gaagaucacg ggccgggucc ccaagaaggu guucaucgag     2340

auggcccgcg ggggcgacga gagcaugaag aacaagaaga ucccggcgcg gcaggagcag     2400

cucaagaagc uguacgacuc cugcggcaac gacaucgcca acuucagcau cgacaucaag     2460

gagaugaagu ccagccuguc ccucuacgac aacaacagcc ugcgccagaa gaagcuguac     2520

cucuacuacc ugcaguucgg gaagugcaug uacaccggcc gggagaucga ccuggaccgc     2580

cuccugcaga acaacgacac cuacgacauc gaccacaucu acccccgguc caaggucauc     2640

aaggacgaca gcuucgacaa ccuggugcuc gugcugaaga acgagaacgc cgagaagucc     2700

aacgaguacc ccgucaagaa ggagauccag gagaagauga aguccuucug gcgcuuccug     2760

aaggagaaga acuucaucag cgacgagaag uacaagcggc ucaccgggaa ggacgacuuc     2820

gagcugcgcg gcuucauggc ccggcagcug gugaacgugc gccagacgac caaggagguc     2880

gggaagaucc uccagcagau cgagcccgag aucaagaucg uguacagcaa ggccgagauc     2940

gcgagcuccu uccgggagau guucgacuuc aucaaggugc gcgagcugaa cgacacccac     3000

cacgccaagg acgccuaccu gaacaucguc gccggcaacg uguacaacac caaguucacg     3060

gagaagccgu accgcuaccu ccaggagauc aaggagaacu acgacgugaa gaagaucuac     3120

agcuacgaca ucaagaacgc cugggacaag gagaacuccc uggagaucgu caagaagaac     3180

auggagaaga acaccgugaa caucacccgg uucaucaagg aggagaaggg ccagcuguuc     3240

gaccucaacc ccaucaagaa gggggagacc agcaacgaga ucaucuccau caagcccaag     3300

guguacaacg gcaaggacga caagcugaac gagaaguacg gguacuacaa gagccugaac     3360

cccgcguacu uccucuacgu cgagcacaag gagaagaaca agcgcaucaa guccuucgag     3420

cgggugaacc ugguggacgu caacaacauc aaggacgaga agucccugau caaguaccuc     3480

guggagaaca agaagcuggu ggagccgcgc gucaucaaga agguguacaa gcgccaggug     3540

auccugauca acgacuaccc cuacagcauc gucgcccucg acagcaacaa gcugauggac     3600

uucgagaacc ugaagccccu cuuccuggag cggaaguacg agaagauccu gaagaacgug     3660

aucaaguucc ucgaggacaa ccagggcaag uccgaggaga acuacaaguu caucuaccug     3720

aagaagaagg accgguacga gaagaacgag acgcuggaga gcgugcgcga gcgguacaac     3780

aucgaguuca acgagaugua cgacaaguuc cucgagaagc ugaacuccaa ggacuacaag     3840

aacuacauca acaacaagaa guaccaggac cugcucgacg ucaaggagaa guucaucaag     3900

cugaaccugu ucgacaaggc cuucacccuc aagagcuucc uggaccuguu caaccgcaag     3960

accauggccg acuucuccaa ggugggccuc accaaguacc uggggaagau ccagaagauc     4020

agcuccaacg ugcugagcaa gaacgagcuc uaccugcugg aggagagcgu cacgggccuc     4080

uucgugaaga agaucgagcu guga                                            4104


<210>  1941
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1941
auggugaaga agaaguacgc caucggcauc gacaucggga ccaacagcgu cggcuggucc       60

guggugaccg acgacuacaa gguccccagc aagaagauga agguguucgg gaacacggag      120

aagcgcuaca ucaagaagaa ccugcugggc acccugcugu ucgacgaggg gaacaccgcc      180

gagaaccggc gccucaagcg gaccgcgcgc cggcgcuaca cgcggcgccg gaaccgcauc      240

cuguaccugc aggagaucuu cgccgaggag aucaacaaga ucgacgacuc cuucuuccag      300

cggcucgacg acagcuuccu gaucguggag gacaagcagg gcuccaagca cccgaucuuc      360

ggcacccugc aggaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacagcuc ccagaaggcc gacauccggc ugaucuaccu cgcccuggcg      480

cacaucauca aguaccgcgg gcacuuccug uucgagggcg accucaagag cgagaacaag      540

gacguccagc accuguucaa cgacuucgug gagauguucg acaagaccgu ggaggggucc      600

uaccugagcg agaaccuccc caacgucgcc gacgugcugg uggagaaggu cuccaagagc      660

cggcgccugg agaacauccu ccacuacuuc cccaacgaga agaagaacgg ccuguucggg      720

aacuuccuga cgcucgcccu gggccugcag ccgaacuuca agaccaacuu cgagcucgcc      780

gaggacgcga agauccaguu cuccaaggag accuacgagg aggaccugga ggagcugcuc      840

ggcaagaucg gggacgacua cgccgaccug uucaucgcca ccaagagccu guacgacggc      900

auccuccugg ccgggauccu guccacgacc gacagcacca ccaaggcgcc ccucuccagc      960

uccaugguga accgguacga ggagcaccag aaggaccugg cccugcucaa gaacuucauc     1020

caccagaacc ugagcgacag cuacaaggag guguucaacg acaagcugaa ggacggcuac     1080

gccggcuaca ucgaggggaa gacgacccag gagaacuucu accgcuucau caagaaggcc     1140

aucgagaaga ucgagggcuc caacuacuuc aucgacaaga ucgaccggga ggacuuccuc     1200

cgcaagcagc ggaccuucga caacgggagc aucccccacc agauccaccu ccaggagaug     1260

cacgccauca uccgccgcca ggccgaguuc uaccccuucc uggucgagaa ccaggacaag     1320

aucgagaaga uccugaccuu ccggaucccg uacuacgugg gcccccuggc gcgcgggaag     1380

uccgaguucg ccuggcugaa ccgcaagagc gacgagaaga uccggcccug gaacuucgac     1440

gagauggugg acaaggagac guccgccgag aacuucauca cccggaugac caacuacgac     1500

caguaccucc ccgaccagaa gguccugccg aagcacagcc ugcuguacga gaaguucgcc     1560

guguacaacg agcucaccaa ggugcgcuac gucacggagc agggcaaguc cuucuucuuc     1620

gacgccaaca ugaagcagga gaucuucgac ggcguguuca agguguaccg gaaggucacc     1680

aaggagaagc ugauggacuu ccuggggaag gaguucgacg aguuccgcau cguggaccuc     1740

cugggccugg acaaggacaa caagagcuuc aacgcguccc ucgggaccua ccacgaccug     1800

aagaagaucg ucagcaagga ccugcucgac aaccccgaga acgaggacau ccuggagaac     1860

guggugcuga cccucacgcu guucgaggac cgggagauga uccgcaagcg gcuggagaag     1920

uacaaggacg uccucaccga ggagcagcgc aagaagcugg agcggcgcca cuacaccggc     1980

ugggggcggc uguccgccaa gcucaucaac ggcauccgcg acaaggugac ccggaagacg     2040

auccuggacu accugaucga cgacggcacc agcaaccgca acuucaugca gcucaucaac     2100

gacgacaccc uguccuucgu ggacgagauc cggcuggccc aggggagcgg cgaggccgag     2160

gacuaccgcg ccgaggucca gaaccucgcg gggucccccg ccaucaagaa gggcauccug     2220

cagagccuga agaucgugga cgagcucauc gaggugaugg gguacgaccc cgagcacauc     2280

gucguggaga uggcccgcga gaaccaguuc accaaccagg gccggcgcaa cucccagcag     2340

cgguacaaga agaucgagaa cgccaucaag aaccuggaca gcaaccugaa cuccaagauc     2400

cucaaggagu acccgacgaa caaccaggcg cugcagaacg accgccuguu ccucuacuac     2460

cugcagaacg gcaaggacau guacaccgac gaggagcugg acaucgacca gcucagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagccu ggacaacaag     2580

gugcugacca aguccgccaa gaaccggggg aaguccgacg acguccccag ccucgagauc     2640

gugcacaaga agaagaacuu cuggaagcag cugcuggaca gccagcucau cucccagcgc     2700

aaguucgaca accugaccaa ggccgagcgg ggcgggcuga ccaacgagga caaggcccgc     2760

uucauccagc ggcagcucgu ggagacgcgc cagaucacca agcacgucgc gcggauccug     2820

gacacccgcu ucaacaccaa gcuggacgag gccggcaacc ggauccgcga ccccaaggug     2880

aacaucauca cgcucaagag caaccuggug ucccaguucc ggaaggacua ccagcuguac     2940

aagguccgcg agaucaacaa cuaccaccac gcccacgacg ccuaccucaa cgccguggug     3000

gcgaccgccc ugcugaagaa guacccgcag cucgcccccg aguucgucua cggcgacuac     3060

cccaaguaca acagcuacaa gucccgcaag agcgccaccg agaaggugcu guucuacucc     3120

aacaucauga acuucuuccg gcgggugcug gucuacagca agaccgggga ggugcgcauc     3180

cggcccguga ucgaggucaa caaggagacg ggcgagaucg ugugggacaa gaaguccgac     3240

uuccgcaccg ugcgcaaggu ccucagcuac ccccagguga acguggucaa gaagguggag     3300

augcagaccg ggggcuucuc caaggagagc auccugcagc acggggacuc cgacaagcug     3360

aucccccgga agaccgagaa guucuaccuc gacacgaaga aguacggcgg cuucgacagc     3420

cccaccaucg ccuacagcgu gcugcugauc gcggacaucg agaaggggaa ggccaagaag     3480

cucaagcgcg ucaaggagcu gaucggcauc accaucaugg agcggauggc cuucgagaag     3540

aaccccaucg aguuccugga gcacaagggg uacaagaaca uccucgagaa gaacaucauc     3600

aagcugccga aguacucccu guucgagcuc gagaacggcc gccggcgccu gcuggccagc     3660

gcgaaggagc uccagaaggg gaacgagaug auccugccgc cccaccuggu gacccuccug     3720

uaccacucca gcaacaucca caagaucacg gagccgaucc accugaacua cgugaacaag     3780

aacaagcacg aguucaagga gcuccugcgg cacaucuccg acuucagcac ccgcuacauc     3840

cuggcccagg accggcucuc caagaucgag gagcuguacg acaagaacga cggcgacgac     3900

aucagcgacc ugaccuccag cuucgucaac cuccugaccu ucacggccau cggcgccccc     3960

gccgcguuca aguuccuggg guccgugauc gaccgcaagc gguacaccag caucgccgag     4020

auccucgagg ccacccugau ccaccagucc gugaccggcc uguacgagac gcgcaucgac     4080

cucagcaagc ucgggggcga cuga                                            4104


<210>  1942
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1942
auggugaaga agaaguacgc caucggcauc gacaucggga ccaacagcgu cggcuggucc       60

guggugaccg acgacuacaa gguccccagc aagaagauga agguguucgg gaacacggag      120

aagcgcuaca ucaagaagaa ccugcugggc acccugcugu ucgacgaggg gaacaccgcc      180

gagaaccggc gccucaagcg gaccgcgcgc cggcgcuaca cgcggcgccg gaaccgcauc      240

cuguaccugc aggagaucuu cgccgaggag aucaacaaga ucgacgacuc cuucuuccag      300

cggcucgacg acagcuuccu gaucguggag gacaagcagg gcuccaagca cccgaucuuc      360

ggcacccugc aggaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacagcuc ccagaaggcc gacauccggc ugaucuaccu cgcccuggcg      480

cacaucauca aguaccgcgg gcacuuccug uucgagggcg accucaagag cgagaacaag      540

gacguccagc accuguucaa cgacuucgug gagauguucg acaagaccgu ggaggggucc      600

uaccugagcg agaaccuccc caacgucgcc gacgugcugg uggagaaggu cuccaagagc      660

cggcgccugg agaacauccu ccacuacuuc cccaacgaga agaagaacgg ccuguucggg      720

aacuuccugg cccucgcccu gggccugcag ccgaacuuca agacgaacuu cgagcucgcg      780

gaggacgcca agauccaguu cuccaaggag accuacgagg aggaccugga ggagcugcuc      840

ggcaagaucg gggacgacua cgccgaccug uucaucgcca ccaagagccu guacgacggc      900

auccuccugg ccgggauccu guccaccacg gacagcacca ccaaggcgcc ccucuccagc      960

uccaugguga accgguacga ggagcaccag aaggaccugg cccugcucaa gaacuucauc     1020

caccagaacc ugagcgacag cuacaaggag guguucaacg acaagcugaa ggacggcuac     1080

gccggcuaca ucgaggggaa gaccacgcag gagaacuucu accgcuucau caagaaggcc     1140

aucgagaaga ucgagggcuc caacuacuuc aucgacaaga ucgaccggga ggacuuccuc     1200

cgcaagcagc ggaccuucga caacgggagc aucccccacc agauccaccu ccaggagaug     1260

cacgccauca uccgccgcca ggcggaguuc uaccccuucc uggucgagaa ccaggacaag     1320

aucgagaaga uccugaccuu ccggaucccg uacuacgugg gcccccuggc ccgcgggaag     1380

uccgaguucg ccuggcugaa ccgcaagagc gacgagaaga uccggcccug gaacuucgac     1440

gagauggugg acaaggagac cuccgccgag aacuucauca cgcggaugac caacuacgac     1500

caguaccucc ccgaccagaa gguccugccg aagcacagcc ugcuguacga gaaguucgcc     1560

guguacaacg agcucaccaa ggugcgcuac gucaccgagc agggcaaguc cuucuucuuc     1620

gacgcgaaca ugaagcagga gaucuucgac ggcguguuca agguguaccg gaaggucacg     1680

aaggagaagc ugauggacuu ccuggggaag gaguucgacg aguuccgcau cguggaccuc     1740

cugggccugg acaaggacaa caagagcuuc aacgccuccc ucgggaccua ccacgaccug     1800

aagaagaucg ucagcaagga ccugcucgac aaccccgaga acgaggacau ccuggagaac     1860

guggugcuga cccucacccu guucgaggac cgggagauga uccgcaagcg gcuggagaag     1920

uacaaggacg uccucacgga ggagcagcgc aagaagcugg agcggcgcca cuacaccggc     1980

ugggggcggc uguccgccaa gcucaucaac ggcauccgcg acaaggugac ccggaagacc     2040

auccuggacu accugaucga cgacggcacg agcaaccgca acuucaugca gcucaucaac     2100

gacgacaccc uguccuucgu ggacgagauc cggcuggccc aggggagcgg cgaggccgag     2160

gacuaccgcg cggaggucca gaaccucgcc gggucccccg ccaucaagaa gggcauccug     2220

cagagccuga agaucgugga cgagcucauc gaggugaugg gguacgaccc cgagcacauc     2280

gucguggaga uggcccgcga gaaccaguuc accaaccagg gccggcgcaa cucccagcag     2340

cgguacaaga agaucgagaa cgcgaucaag aaccuggaca gcaaccugaa cuccaagauc     2400

cucaaggagu acccgaccaa caaccaggcc cugcagaacg accgccuguu ccucuacuac     2460

cugcagaacg gcaaggacau guacacggac gaggagcugg acaucgacca gcucagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagccu ggacaacaag     2580

gugcugacca aguccgccaa gaaccggggg aaguccgacg acguccccag ccucgagauc     2640

gugcacaaga agaagaacuu cuggaagcag cugcuggaca gccagcucau cucccagcgc     2700

aaguucgaca accugaccaa ggccgagcgg ggcgggcuga ccaacgagga caaggcgcgc     2760

uucauccagc ggcagcucgu ggagacccgc cagaucacga agcacgucgc ccggauccug     2820

gacacccgcu ucaacaccaa gcuggacgag gccggcaacc ggauccgcga ccccaaggug     2880

aacaucauca cccucaagag caaccuggug ucccaguucc ggaaggacua ccagcuguac     2940

aagguccgcg agaucaacaa cuaccaccac gcccacgacg ccuaccucaa cgcgguggug     3000

gccacggccc ugcugaagaa guacccgcag cucgcccccg aguucgucua cggcgacuac     3060

cccaaguaca acagcuacaa gucccgcaag agcgccaccg agaaggugcu guucuacucc     3120

aacaucauga acuucuuccg gcgggugcug gucuacagca agaccgggga ggugcgcauc     3180

cggcccguga ucgaggucaa caaggagacc ggcgagaucg ugugggacaa gaaguccgac     3240

uuccgcacgg ugcgcaaggu ccucagcuac ccccagguga acguggucaa gaagguggag     3300

augcagaccg ggggcuucuc caaggagagc auccugcagc acggggacuc cgacaagcug     3360

aucccccgga agaccgagaa guucuaccuc gacaccaaga aguacggcgg cuucgacagc     3420

cccacgaucg cguacagcgu gcugcugauc gccgacaucg agaaggggaa ggccaagaag     3480

cucaagcgcg ucaaggagcu gaucggcauc accaucaugg agcggauggc cuucgagaag     3540

aaccccaucg aguuccugga gcacaagggg uacaagaaca uccucgagaa gaacaucauc     3600

aagcugccga aguacucccu guucgagcuc gagaacggcc gccggcgccu gcuggcgagc     3660

gccaaggagc uccagaaggg gaacgagaug auccugccgc cccaccuggu gacccuccug     3720

uaccacucca gcaacaucca caagaucacc gagccgaucc accugaacua cgugaacaag     3780

aacaagcacg aguucaagga gcuccugcgg cacaucuccg acuucagcac gcgcuacauc     3840

cuggcccagg accggcucuc caagaucgag gagcuguacg acaagaacga cggcgacgac     3900

aucagcgacc ugaccuccag cuucgucaac cuccugaccu ucaccgccau cggcgccccc     3960

gcggccuuca aguuccuggg guccgugauc gaccgcaagc gguacacgag caucgccgag     4020

auccucgagg ccacccugau ccaccagucc gugaccggcc uguacgagac ccgcaucgac     4080

cucagcaagc ucgggggcga cuga                                            4104


<210>  1943
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1943
auggacaaga aguacagcau cggccuggac aucgggaccu ccagcaucgg cuucgugggg       60

gucgacgaga acggcuggcc ccucaacauc aaggggaagc gcgccaucgg cgcccaccug      120

uuccgggagg gcgaggcggc cgccgagacc cgcggguucc ggacgacccg ccggcgcuac      180

aaccggcgca aguggcggcu gcgccuccug cgggagaucu ucgacgagcc gaucucccgc      240

guggacccca ccuucuucgc ccggaugaag gagagcuccg ugagccccaa ggaccccaac      300

uacgacggcc uggccaagga gauccucauc ccgcgcgaca aggacgggaa gauccuguac      360

cccaccaucu accaccuccg gcacgcgcuc auguacgagg accaccaguu cgacauccgc      420

gagaucuacc uggccaucca ccacaucguc aaguaccggg gccacuuccu gcucgacggg      480

gccgccaaga acuucgaguc cagcaaccug gaccugaagu ccgacaucca cgagaucaac      540

gcgauccucg ugggcaucuu cggcaacgag cagguccagu ucaacgacca guacguggag      600

aagcugaagg aggugcugcu cgacagcacg cagucccgca gcgaccggca gaaggccgcc      660

gccaaggugc ugcugcccac caacgaccuc gacaaggaca ccaccaagau ccugaagacg      720

gucuccaccg agaucaccaa ggccaucacc gggcugaagg cgaaguucuu cgugaucacg      780

ggcaacgaca ucgccaagga ccagcagaag gacuggaccu ucagcaucga cacccucgac      840

gagaagaugu ccgugaucga gcccagcuuc uccgagaaga acuucagccu gaucaccauc      900

cugcagaagc ucuacuccag cgucacgcug gccgggaucg ugccgaacgg ccugagccuc      960

uccgaggcca ugauccagaa guacgaccgc cacgccagcg accuggugcu gcucaagaag     1020

uacugggaca ccaagcccaa gguccugaag caccagcugc agaacaccua cgcggaguac     1080

aucgagaucc accagguggu ggacgaguuc uacuccgacc ucagcaagcu gaucaagaac     1140

gacgacgaca cccuggccca ggagaucaag uccaagauca cgaucggcaa cuuccucccc     1200

aagcagcgga ccaccgagaa cggggucauc cccuaccagc ugcagcagcg cgagcuggac     1260

accaucauca agaaccagca ggaguacuac ccguggcucg ccgaggagaa ccccguggcc     1320

gaccaccaga ucgccuuccc cuacaagcug gacgagcugg ugggcuuccg cauccccuac     1380

uacgucgggc cgaugaucgc gaaggaggac acgagcaacu ccaagaacag caaguucgcc     1440

uggaugaucc ggaagcaguc cggcgaggug acccccugga acuucgacga gaagaucgac     1500

cgcaccgcca gcgccaccgc cuucaucgac cggaugacga ccaccgacac cuaccucauc     1560

ggggaggacg ugcugcccaa gcagucccug aucuaccagc gcuucgaggu ccucaacgag     1620

cugaacaaga ucagcaucga cggccagccc aucacgcgga agcagaagca guccguguac     1680

acccacgucu ucgagaagaa gaagaccguc caggugaagg acgugcagaa guuccuggug     1740

agcuccggcg aggugaugag ccugccgaag aucgggggcc ucgcgaacga gaagcaguuc     1800

aacuccagcc uguccacccu guacgacuac aacaagauca uccccgacgc ccucgaggac     1860

cgcaacaagg ucgacgacau cgagaagauc auccugugga gcacgaucuu cgaggacaac     1920

agcaucuucg uggagaagcu ggacuccauc gacuggcuca ccagcuccca gcggaagcgc     1980

cuggcccaga agcgguaccg cggguggggc cggcugagcc uccgccugcu gaccaccuuc     2040

aagaacaagg acggguacuc cauccucgac gagcugugga acagcaacca caacuucaug     2100

gagauccagg cccaguccga cuucagcgag cagaucgcga ccgccaacaa caacaaccug     2160

ucccaggugc uccccgccaa gugggagccc ggccggaaga accuggccag ccuggacgag     2220

uucaucucca accucuacac gagcccgcag aacaagaagg cccugcgcaa ggcgauccgg     2280

gucgugcagg acaugaugac cgccgccggc cacaugcccg agaagaucuu caucgaggug     2340

gcccgcgaca ccuccaacag ccaccagcag accaucgccc ggcagcgcca gcugcagcag     2400

cucuaccagu ccgaggcgaa ggaggucauc gacagcuccg ugcggagcga gcuggacgac     2460

auccugaaga accgcgccaa caucaaggac cagcuguucc uguacuucca gcagggcggg     2520

cgcgaccugu acacggggaa ggccaucaac aucgacaagc ucagccugua ccacaucgac     2580

cacauccugc cccagucccu cgugaaggac gacagccugg acaaccgggu ccuggugucc     2640

cagaagacca acgaggacaa gggcgugacc uucgucagcg acaaguacau gaacaacaug     2700

cuccccaccu ggaagcgcug gcacgagaug gggcugaucu ccgcccggaa gcugaagaac     2760

cucacgaugc gcccggacga gaucgacaag uacgcccccg gcuucaucca gcggcagcug     2820

guggagaccc gccaggugac caagcugguc aucgagcucc uggacgcgcu gcucggcaac     2880

agccagaccg agaucaucac gaucaaggcc ucccugagcc accaguuccg gcagcaguuc     2940

gaccugguga agauccgcga ggugaacgac aaccaccacg ccuucgacgc cuuccucgcg     3000

gcccggaucg ggaccuaccu guacaagcgc uaccccaagc ugcggccgua cuucgucuac     3060

ggccaguaca ugcagaacca caagaaccuc aaguccauca accugaucaa gacccuggac     3120

agcgacaagg ccaucgugga cgaggggacc ggcgagaucc ucugggacaa gaaguccgac     3180

cuggccaaga ugaucaagau cuacaacuac cagcgccugc ucaucacgca cgagagccgg     3240

accuccuucg gcgccaugua caagcagaag auccugaagg cgaccgacgc caagcagaac     3300

gggggcgggu ucaagcuggu gcccaccaag cgcaacuggc ccgucgacau cuacggcggg     3360

uacacgggcc agaccgccgc cuaccucacc aucgugcggg uguacagcaa gaagaccucc     3420

gagcugcgcg uccugcccau cuacacgaac aaggugcagc agaucgagaa ggccaugacc     3480

cagggcgaca aggcgaccau gcucgccuuc aagaagguga ucgccccgca gcuggggaag     3540

gagaacaaga agacccacga gaucaagauc cccgaguucg acgucgugau ccccaaggug     3600

cugcucgacc agcuggucau cgacgacauc gccggcagcc cccaccgguu ccgccugggg     3660

acggacaccu acuaccacaa cucccagcag cuguuccucc cgcugagcau gcagcggcag     3720

cucauggacc gcgaguccga cgacaaggug uaccuggaca ucuucaacga cgugcugaag     3780

caggucgaca gcuacuucaa ccucuacgac gugaacggcu uccgggccgc gcugcagaag     3840

uccaagaagg ccuucgagca gcugcccauc aacgacuucc ccaaggacgu gcccgacaac     3900

aagaagaaga aggggaagcg cagcauccuc aaggagaucu ucaucggccu gcacgccaac     3960

gccacccugg gcaaccucaa gcagaugggg aucaagaccg acuucggcaa gcugcagaag     4020

uccaacgggc ugcgcaucac ggagaacugc aagaucaucc accagagccc gaccggccuc     4080

uucgagcgga ucguccccau caaguga                                         4107


<210>  1944
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1944
augagccgcc ccuacaacau cggccuggac aucgggaccu ccagcaucgg cugguccgug       60

gucgacgacc agagcaagcu cguguccgug cgggggaagu acggcuacgg gguccgccug      120

uacgacgagg gccagaccgc cgccgagcgg cgcagcuucc ggacgacccg ccggcgccug      180

aagcggcgca aguggcggcu cggccugcug cgcgagaucu ucgagccgua caucaccccc      240

guggacgaca ccuucuuccu ccggcagaag caguccaacc ugagccccaa ggaccagcgc      300

aagcuguacc cccagacguc ccucuucaac gaccggaccg accgcgcguu cuacgacgac      360

uacccgacca ucuaccaccu ccgguacaag cugaugaccg agaagcgcca guucgacauc      420

cgggagaucu accuggccau gcaccacauc gugaaguacc gcgggcacuu ccugaacgag      480

gcccccguca gcuccuucaa gagcuccgag aucaaccugg uggcccacuu cgaccggcuc      540

aacacgaucu ucgccgaccu guucagcgag uccggcuucc agcuggagac cgacaagcuc      600

gcggagguga aggcccugcu gcucgacaac cagcagagcg ccuccaaccg ccagcgccag      660

gcccugagcc ugaucuacac ccccuccacc aacaaggcgg ucgagaagca gaacaaggcc      720

aucgccacgg agcuccugaa ggccauccug gggcucaagg ccaaguucaa cgugcugacc      780

ggcaucgagg cggaggacgu gaaggccugg acccugaccu ucaacgccga gaacuucgac      840

gaggagaugg ucaagcucga gagcagccug gacgacaacg cccaccagau caucgagucc      900

cugcaggagc ucuacagcgg ggugcugcug gccggcaucg ugcccgagaa ccagucccuc      960

agccaggcga ugaucacgaa guacgacgac caccagaagc accugaagau gcugaaggcc     1020

guccgggagg cccucgcccc ggaggaccgc cagcggcuga agcaggccua cgaccaguac     1080

guggacggcc aggagaacac caaggcguac uccaaggagg acuucuacgg ggacaucacc     1140

aaggcccuga agaacaaccc cgaccacccc aucgugagcg agaucaagaa gcucaucgag     1200

cuggaccagu ucaugcccaa gcagcgcacc aaggacaacg gcgccauccc gcaccagcug     1260

caccagcagg agcucgaccg gaucaucgag aaccagcagc aguacuaccc cuggcuggcc     1320

gagcugaacc ccaacuccaa gcgccagacg gucgccaagu acaagcucga cgagcuggug     1380

gcguuccggg ugcccuacua cgucgggccg cugaucaccg ccgagcagca gcgccagagc     1440

uccgacgcca aguucgccug gcugauccgg aaggcggagg gccgcaucac ccccuggaac     1500

uucgacgaca agguggaccg gcaggccagc gccaacgagu ucaucaagcg caugaccacg     1560

accgacaccu accuccuggc cgaggacgug cugcccaagc agucccucau cuaccagcgg     1620

uucgaggucc ugaacgagcu gaacggccuc aagaucgacg accagcccau caccacggag     1680

cugaagcagg ccaucuucac cgaccuguuc augcagaaga ucagcgugac cgucaagaac     1740

auccaggacu accucguguc cgagaagcgc uacgcgagcc ggccggccau caccgggcug     1800

uccgacgaga acaaguucaa cagccgccug uccacguacc acgaccucaa gaugaucgug     1860

ggcgacgccg ucgacgacgu ggacaagcag gccgaccugg agaagugcau cgaguggagc     1920

accaucuucg aggacgggaa gaucuacucc gccaagcuga acgagaucga cuggcucacc     1980

gaccagcagc gggugcagcu ggcggccaag cgcuaccggg gcugggggcg ccugagcgcc     2040

aagcuccuga cccagaucgu caacgccaac ggccagcgca ucauggaccu gcucugggac     2100

acgaccgaca acuucaugcg gaucgugcac agcgaggacu ucgacaagcu gaucaccgag     2160

gccaaccaga ugaugcuggc ggagaacgac gugcaggacg ucaucaacga ccucuacacc     2220

agcccccaga acaagaaggc ccugcgccag auccugcucg uggugaacga cauccagaag     2280

gccaugaagg gccaggcccc cgagcggauc cugaucgagu ucgcgcgcga ggacgagguc     2340

aacccccggc uguccgugca gcgcaagcgc cagguggagc aggucuacca gaacaucucc     2400

aacgagcucc ugaacaacac cgagauccgg aacgagcuga aggaccucag caacuccgcc     2460

cugagcaaca cgcggcuguu ccucuacuuc augcagggcg ggcgcgacau guacaccggg     2520

gacucccuga acaucgaccg gcugagcacc uacgacaucg accacauccu cccgcagucc     2580

uucgugaagg acaacagccu ggacaaccgc gugcuggucu cccagaagau gaaccggagc     2640

aaggccgacc aggugcccac cgacuucacg uccguggagc ucggcaagaa gaugcagcug     2700

cagugggagc agaugcugcg cgccgggcuc aucaccaaga agaaguacga caaccugacc     2760

cugaaccccg accacaucag caaguacgcc augaagggcu ucaucaaccg gcagcucguc     2820

gagacccgcc aggugaucaa gcuggcgacg aaccugcuca uggagcagua cggcgaggac     2880

aacaucgagc ugaucaccgu gaaguccggg cugacccacc agaugcggac cgaguucgac     2940

uuccccaaga accgcaaccu caacaaccac caccacgccu ucgacgccua ccugacggcc     3000

uucgucggcc uguaccuccu gaagcgguac ccgaagcuga agcccuacuu cguguacggg     3060

gaguaccaga aggccagcca gcaggacaag uggcgcaacu ucaacuuccu caacggccug     3120

aagaaggacg agcuggugga cgagaacacc gaggcgguca ucugggacaa ggaguccggg     3180

cucgccuacc ugaacaagau cuaccaguuc aagaagaucc uggugacccg ggaggugcac     3240

gagaacagcg gcgcccucuu caaccagacc cuguacgccg ccaaggacga caaggcgagc     3300

ggccagggcg ggaagcagcu gauccccgcc aagcaggacc gcccgacggc ccucuacggg     3360

ggcuacuccg gcaagaccag cgccuaccug ugccugguca agaucaagaa caaguccgag     3420

uacaucuacc gcaucuucgg ggugaacacc agcuggucca gcgagcucaa gcagauccag     3480

gaccccgcgg agcggcuggu gcgccuggag aagcuccuga cgccccagcu gaccaucaac     3540

aagaagaaga agggcgagcc cgucccggug accaaggacu ucaagaucau ccucccccgg     3600

auccugauca accagaaguu cuacucccgc gggcaggaca ucagcaucgc cuccgaguuc     3660

uaccugcaca acgagcagga gcucgugcug gacaagagcg acaucaagau ccugaacggc     3720

gccaucaagg ucaacaacuc caacaagcag gucaucaacc ucuucaacca cauccuggag     3780

caggucaacc aguacuuccc cguguacgac aucuacaaga gccgggaggu gcugaacucc     3840

agcaaggaga ucuucgagaa ccucaccugg aacgaccagu ggcgcaaccg gaagcugauc     3900

aacguggggc agaagaccau ccugaaccgc auccucaucg gccugcacgc caacgccgcg     3960

cggaucaaga ucuccgagcu gggcuucaag aacgacuucg ggcuccugac gcagaccagc     4020

ggcaucaccu ucacccccga cacgcagauc aucuaccagu ccccgaccgg gcuguucgag     4080

cgccgggugg cccugcgcga ccucuga                                         4107


<210>  1945
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1945
augaagcgca gcuacaacau cggccuggac aucgggacca acuccaucgg cugggccauc       60

guggacgacc agagcaagcu caucuccguc aaggggaagu acggcuacgg ggugcggcug      120

uacgacgagg gccagaccgc cgcggagcgc cggagcuucc gcacgacccg gcgccggcug      180

aagcgccgga aguggcgccu cggccugcug cgggagaucu ucgagcccua caucaccccg      240

aucgacucca ccuucuuccu ccgccagaag cagagcaacc ugucccccaa ggaccagcgg      300

aagcuguacc cccagacgag ccucuucaac gaccgccccg acgccgacuu cuaccagcag      360

uacccgacca ucuaccaccu ccgguaccag cugaugaccg agaagcgcca guucgacauc      420

cgggagaucu accucgccau gcaccacauc gugaaguacc gcgggcacuu ccugaacgag      480

gcccccgucg ccuccuucaa gagcuccgag aucaaccugg cggagcacuu cgaccagcuc      540

aacaccaucu ucgccgaccu guuccgggag agcggcuucc agcuggagac ggacgagcuc      600

guggccguga aggcccugcu gauggacaac cagcaguccg cgagcaaccg ccagcggaag      660

gcccucccca agcuguacac ccccuccgcc gacaagagca ucgagaagca gcgcaaggcc      720

aucgccaccg agauccugaa ggcgauccuc gggcugaagg ccaaguucaa cgucgugacc      780

ggcaucgagg ccgaggacgu gaaggccugg gcccugacgu ucaacgcgga gaacuucgac      840

gaggagcucg ccaagcugga guccagccug gacgacaacg ggcacgaggu caucggcauc      900

cuccaggagc uguacuccgg cauccugcuc gccgggaucg ugccggagaa ccagagccug      960

agccaggcca ugaucaccaa guacgaggag caccaggccc accugaagau gcucaaggcg     1020

gugcgcgacg accuggccac cgccgaccag cagaagcuga agaaggccua cgaccaguac     1080

aucgacggcc aggagaacac caaggccuac uccaaggagg cguucuacga cgacgucacg     1140

aaggcccuca aggacuacag cgagcagccc cugguguccg agauccagaa gcugaucgag     1200

cucgaccagu ucaugcccaa gcagcggacc aaggacaacg gggccauccc ccaccagcug     1260

caccagcagg agcuggaccg caucaucgag aaccagcagc aguacuaccc guggcucgcc     1320

gagcugaacc ccaaccccaa gcggcagacc guggcgaagu acaagcugga cgagcucguc     1380

gccuuccgcg ugcccuacua cgugggcccg cugaucaccg ccgagcagca gcagcagagc     1440

uccaacgcca aguucgccug gaugauccgg aagacggagg gccgcaucac ccccuggaac     1500

uucgacgaca aggucgaccg gcaggcgagc gccaacgagu ucaucaagcg caugaccacc     1560

acggacaccu accugcuggc cgaggacgug cuccccaagc agucccugau cuaccagcgg     1620

uucgaggugc ugaacgagcu caacgggcug aagaucgacg accagcccau caccaacgag     1680

cugaagcagg ccaucuucaa cgaccucuuc augcagaaga ccagcgucuc cgugaagaac     1740

auccaggacu accuggugag cgagaagcgc uacgccuccc ggccggcgau cacgggccug     1800

agcgacgaga agaaguucaa cucccgccuc agcaccuacc acgaccugaa ggucaucgug     1860

ggggacgccg ucgacgacgu ggacaagcag gccgaccugg agaagugcau cgaguggucc     1920

accaucuucg aggacggcaa gaucuacagc gccaagcuca acgagaucac cuggcugacg     1980

gagcagcagc gggggcagcu ggccaccaag cgcuaccggg gcuggggccg ccucuccgcg     2040

aagcugcuga cccagaucgu gaacgccaac gggcagcgga ucauggaccu ccugugggac     2100

accacggaca acuucaugcg caucguccac uucgaggacu ucgacaagcu gaucaccgag     2160

gccaaccaga uggugcucgc cgagaacgac gugcaggacg ucaucaacga ccuguacacc     2220

agcccccaga acaagaaggc gcugcggcag auccuccugg uggugaacga cauccagaag     2280

gccaugaagg gccaggcccc cgagcgcauc cugaucgagu ucgcccgcgg ggcccagaag     2340

gacaaguccc ucaccguccg gcgcaagcgg aagcuggacg agauguacag ccagaucagc     2400

gacgcggugc uggccgacga guccgugcgc caggagcuca agagcuccaa ccaggacgac     2460

cuggucaagg agaaggugua ccuguacuuc augcagggcg ggcgggaccu cuacaccggc     2520

aacgcccuga acaucgaccg ccugagcgac uaccacgugg accacaucau cccccaggcc     2580

uacaucaagg acgacucccu cgacaacaag gcccugguca gcucccggau gaacgagauc     2640

aaggcggacc gcgugccgcu ccaggugguc cagaagcagu ucggcaagaa cauggccagc     2700

gagugggggu acaugcaccg ggccggccug aucucccgca agaaguacga caaccugaug     2760

uucaaccccg agaaccugaa caaguaccag aucaaccggu ucaucagccg ccagcucgug     2820

gagacgcagc aggucaucaa gcugguggcc aaccugcucu ccaaccagua cgagggggac     2880

aacgugaaga ucaucaccgu caagagcaag cugacccaga ccauccgguc cgaguucgac     2940

uuccccaaga accgcgaggu gaacaacuac caccacgccu ucgacgcgua ccugacggcc     3000

uucgugggca uguaccuccu gaagcgguac cccaagcuga gcuccuacuu caccuacggg     3060

gaguaccuca agggcaacca gcaggacaag uggagcuccu ucaacuuccu gaacgaccug     3120

aagcgcgcca agcucgagga caagaccaac aaggagaucu ucuggcacaa ggagagcggc     3180

cuggccuacc ugaacaagau cuaccaguuc aagaagaucc ucgugacccg ggagguccac     3240

gagaacagcg gggcgcuguu caaccagacg cuguacgccg ccaaggacga caaggccucc     3300

ggccagggcg ggaagcagcu caucccggcc aagcaggcgc gccccaccgc ccuguacggc     3360

ggguacagcg gcaagaccgu ggccuacaug ugcaucgugc ggaucaagaa caagaagggg     3420

gaccuguaca aggucugcgg cguggagacc uccuggcucg cccagcugaa gcagcugaag     3480

gacgaggaca gccagaaggc cuuccucaag cagaagauca gcccccaguu cacgaaggcg     3540

aagaagcaga aggggaccau cgugaagguc guggaggacu ucgaggugau cgccccgcac     3600

auccugauca accagcgccu guucgacaac ggccaggagc ucacccuggg cagcgccacc     3660

uacaagcaca acgagcagga gcugauccuc gacaagacgg ccgucaagcu gcugaacggg     3720

gagcuccccc uggcccaguc cgaggagcug gcggagcucg uguacgacga gauccuggac     3780

caggugaugc acuacuuccc ccuguacgac accaaccagu uccgggccaa gcuccuggcc     3840

ggcaagaccg ccuucaugca gcugcccugg aaguccaacc cggacgacaa caagaccguc     3900

cagaucacgc agcccaucgu gcucaagaag auccugcagg ggcugcacgc cgacgcgggc     3960

cgcguguccc ucaaggagcu ggggaugacc accgacuucg gcaugcugca gaaggccacc     4020

ggcaucacgu ucacccccga cacccagauc aucuaccaga gccccaccgg gcuguucgag     4080

cgccgggucg cccugcgcga ccuguga                                         4107


<210>  1946
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1946
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcuggggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcaucugc      240

uaccugcagg agaucuucuc caacgagaug gccaaggucg acgacagcuu cuuccaccgg      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcgccaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccggaag      420

aagcucgugg acagcaccga caaggcggac cugcgccuga ucuaccucgc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

guggacaagc uguucaucca gcugguccag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gcggcgugga cgcgaaggcc auccuguccg cccgccugag caagucccgg      660

cgccucgaga accugaucgc ccagcugccg ggggagaaga agaacggccu cuucgggaac      720

cugaucgccc ugagccucgg ccugacgccc aacuucaagu ccaacuucga ccuggcggag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg ggugaacacc gagaucacca aggccccgcu guccgccagc      960

augaucaagc gcuacgacga gcaccaccag gaccucacgc ugcugaaggc ccucguccgg     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc agagcaagaa cggguacgcg     1080

ggcuacaucg acgggggcgc cucccaggag gaguucuaca aguucaucaa gccgauccug     1140

gagaagaugg acggcaccga ggagcuccug gugaagcuga accgcgagga ccugcuccgg     1200

aagcagcgca ccuucgacaa cgggagcauc ccccaccaga uccaccuggg cgagcugcac     1260

gccauccucc gccggcagga ggacuucuac cccuuccuga aggacaaccg cgagaagauc     1320

gagaagaucc ugaccuuccg gauccccuac uacguggggc cgcucgcccg cggcaacucc     1380

cgguucgccu ggaugacgcg caagagcgag gagaccauca cccccuggaa cuucgaggag     1440

gucguggaca agggggcguc cgcccagagc uucaucgagc ggaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu gcugcccaag cacucccucc uguacgagua cuucacgguc     1560

uacaacgagc ugaccaaggu gaaguacgug accgagggca ugcgcaagcc ggccuuccuc     1620

agcggcgagc agaagaaggc caucgucgac cugcuguuca agaccaaccg gaaggugacg     1680

gugaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacuc cgucgagauc     1740

agcggggugg aggaccgcuu caacgcgucc cugggcaccu accacgaccu gcucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucguc     1860

cucacccuga cccuguucga ggaccgggag augaucgagg agcgccucaa gacguacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc gccgguacac cggguggggc     1980

cgccucagcc ggaagcugau caacgggauc cgcgacaagc aguccggcaa gaccauccug     2040

gacuuccuca agagcgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

ucccugaccu ucaaggagga cauccagaag gcccagguga gcgggcaggg cgacucccuc     2160

cacgagcaca ucgccaaccu ggcggggagc cccgccauca agaagggcau ccugcagacg     2220

gucaaggugg uggacgagcu cgucaaggug auggggcgcc acaagcccga gaacaucgug     2280

aucgagaugg cccgcgagaa ccagaccacc cagaagggcc agaagaacuc ccgggagcgc     2340

augaagcgga ucgaggaggg caucaaggag cuggggagcc agauccugaa ggagcacccc     2400

gucgagaaca cccagcucca gaacgagaag cuguaccugu acuaccucca gaacggccgc     2460

gacauguacg uggaccagga gcuggacauc aaccggcuga gcgacuacga cguggaccac     2520

aucgucccgc aguccuuccu caaggacgac agcaucgaca acaaggugcu gacgcgcucc     2580

gacaagaacc ggggcaagag cgacaacgug cccuccgagg aggucgugaa gaagaugaag     2640

aacuacuggc gccagcugcu caacgccaag cugaucaccc agcggaaguu cgacaaccug     2700

accaaggccg agcgcggggg ccucagcgag cuggacaagg cgggguucau caagcggcag     2760

cugguggaga cccgccagau caccaagcac gucgcccaga uccucgacuc ccggaugaac     2820

acgaaguacg acgagaacga caagcugauc cgcgagguga aggugaucac ccucaagagc     2880

aagcucgucu ccgacuuccg gaaggacuuc caguucuaca aggugcgcga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gccguggucg gcaccgcgcu gaucaagaag     3000

uaccccaagc ucgagagcga guucguguac ggggacuaca agguguacga cguccggaag     3060

augaucgcca aguccgagca ggagaucggc aaggccaccg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacggagauc acccuggcga acggggagau ccgcaagcgg     3180

ccccugaucg agaccaacgg cgagaccggc gagaucgugu gggacaaggg gcgcgacuuc     3240

gccacggugc ggaagguccu cuccaugccc caggugaaca ucgugaagaa gaccgagguc     3300

cagaccggcg gguucagcaa ggaguccauc cugcccaagc gcaacagcga caagcugauc     3360

gcccgcaaga aggacuggga ccccaagaag uacggcgggu ucgacucccc caccguggcc     3420

uacagcgugc ucgucguggc caagguggag aagggcaaga gcaagaagcu gaaguccguc     3480

aaggagcugc ucggcaucac gaucauggag cggagcuccu ucgagaagaa cccgaucgac     3540

uuccuggagg cgaaggggua caaggaggug aagaaggacc ugaucaucaa gcuccccaag     3600

uacagccugu ucgagcugga gaacggccgc aagcggaugc ucgccuccgc cggggagcug     3660

cagaagggca acgagcuggc ccuccccagc aaguacguga acuuccugua ccuggccucc     3720

cacuacgaga agcucaaggg gagcccggag gacaacgagc agaagcagcu guucgucgag     3780

cagcacaagc acuaccugga cgagaucauc gagcagaucu ccgaguucag caagcgcgug     3840

auccucgcgg acgccaaccu ggacaaggug cuguccgccu acaacaagca ccgggacaag     3900

cccauccgcg agcaggccga gaacaucauc caccucuuca cccugaccaa ccugggcgcc     3960

cccgcggccu ucaaguacuu cgacaccacg aucgaccgga agcgcuacac cagcaccaag     4020

gagguccucg acgccacccu gauccaccag uccaucacgg gccuguacga gacccggauc     4080

gaccucagcc agcucggggg cgacuga                                         4107


<210>  1947
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1947
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aagcucaagg ggcugggcaa cacggaccgc      120

cacgggauca agaagaaccu gaucggcgcc cuccuguucg acuccggcga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcaucugc      240

uaccuccagg agaucuucag caacgagaug gccaaggugg acgacuccuu cuuccaccgg      300

cuggaggaga gcuuccuggu cgaggaggac aagaagcacg agcgccaccc gaucuucggg      360

aacaucgugg acgagguggc cuaccacgag aaguacccca ccaucuacca ccuccggaag      420

aagcuggcgg acuccaccga caaggucgac cugcgccuca ucuaccuggc ccuggcccac      480

augaucaagu uccggggcca cuuccucauc gagggggacc ugaaccccga caacagcgac      540

guggacaagc uguucaucca gcucgugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgccu cccgcgucga cgcgaaggcc auccugagcg cccggcucuc caagagccgc      660

cggcuggaga accugaucgc ccagcucccg ggcgagaaga agaacgggcu guucggcaac      720

cugaucgccc ucucccuggg ccugacgccc aacuucaaga gcaacuucga ccucgcggag      780

gacgccaagc ugcagcuguc caaggacacc uacgacgacg accucgacaa ccugcuggcc      840

cagaucgggg accaguacgc cgaccucuuc cuggccgcga agaaccugag cgacgccacc      900

cuccuguccg acauccugcg cgugaacagc gagaucacca aggcgccccu cuccgccagc      960

augaucaagc gguacgacga gcaccaccag gaccugacgc ugcucaaggc ccuggugcgc     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc agagcaagaa cggcuacgcc     1080

ggguacaucg acggcggcgc cucccaggag gaguucuaca aguucaucaa gccgauccuc     1140

gagaagaugg acgggaccga ggagcugcug gccaagcuga accgggagga ccuccugcgc     1200

aagcagcgca ccuucgacaa cggcagcauc cccuaccaga uccaccuggg ggagcuccac     1260

gccauccugc ggcgccagga ggacuucuac cccuuccuga aggacaaccg ggagaagauc     1320

gagaagaucc ucaccuuccg cauccccuac uacgucggcc cgcuggcccg ggggaacucc     1380

cgcuucgcgu ggaugacgcg caagagcgag gagaccauca cccccuggaa cuucgaggag     1440

gugguggaca agggcgccuc cgcccagagc uucaucgagc ggaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu ccuccccaag cacucccugc uguacgagua cuucacggug     1560

uacaacgagc ucaccaaggu gaaguacguc accgagggca ugcggaagcc ggccuuccug     1620

agcggggagc agaagaaggc gaucguggac cugcuguuca agaccaaccg caaggugacg     1680

gucaagcagc ugaaggagga cuacuucaag aagaucgagu gcuucgacuc cguggagauc     1740

agcggcgugg aggaccgguu caacgccucc cuggggaccu accacgaccu ccugaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccucga ggacaucguc     1860

cugacccuga cccucuucga ggaccgcgag augaucgagg agcggcugaa gacguacgcc     1920

caccuguucg acgacaaggu gaugaagcag cucaagcgcc ggcgcuacac cggcuggggg     1980

cggcugagcc gcaagcugau caacggcauc cgggacaagc aguccggcaa gaccauccuc     2040

gacuuccuca agagcgacgg guucgccaac cgcaacuuca ugcagcugau ccacgacgac     2100

ucccucaccu ucaaggagga cauccagaag gcccagguca gcggccaggg ggacucccug     2160

cacgagcaca ucgcgaaccu ggccggcagc cccgccauca agaaggggau ccuccagacg     2220

gugaaggugg ucgacgagcu ggugaaggug augggccgcc acaagcccga gaacaucguc     2280

aucgagaugg cccgggagaa ccagaccacc cagaagggcc agaagaacuc ccgcgagcgg     2340

augaagcgca ucgaggaggg gaucaaggag cugggcagcg acauccucaa ggaguacccc     2400

guggagaaca cccagcugca gaacgagaag cuguaccucu acuaccugca gaacgggcgg     2460

gacauguacg uggaccagga gcuggacauc aaccgccuca gcgacuacga cgucgaccac     2520

aucgugccgc aguccuuccu gaaggacgac agcaucgaca acaaggugcu gacgcggucc     2580

gacaagaacc gcggcaagag cgacaacguc cccuccgagg agguggugaa gaagaugaag     2640

aacuacuggc ggcagcuccu gaacgccaag cugaucaccc agcgcaaguu cgacaaccuc     2700

accaaggcgg agcggggcgg gcugagcgag cuggacaagg ucggcuucau caagcgccag     2760

cucguggaga cccggcagau caccaagcac guggcccaga uccuggacuc ccgcaugaac     2820

acgaaguacg acgagaacga caagcugauc cgggaggucc gcgugaucac ccucaagagc     2880

aagcuggugu ccgacuuccg gaaggacuuc caguucuaca agguccgcga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gccguggugg ggaccgcgcu caucaagaag     3000

uaccccaagc uggagagcga guucgucuac ggcgacuaca agguguacga cgugcggaag     3060

augaucgcca aguccgagca ggagaucggg aaggccaccg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gacggagauc acccuggcga acggcgagau ccgcaagcgg     3180

ccccucaucg agaccaacgg cgagaccggg gagaucgucu gggacaaggg ccgcgacuuc     3240

gccacggugc gcaaggugcu guccaugccg caggucaaca ucgugaagaa gaccgaggug     3300

cagaccgggg gcuucagcaa ggaguccauc cugcccaagc ggaacagcga caagcucauc     3360

gcccgcaaga aggacuggga ccccaagaag uacgggggcu ucgacucccc caccgucgcc     3420

uacagcgugc ugguggucgc caagguggag aagggcaaga gcaagaagcu gaaguccgug     3480

aaggagcucc uggggaucac gaucauggag cggagcuccu ucgagaagga cccgaucgac     3540

uuccuggagg cgaagggcua caaggagguc cgcaaggacc ucaucaucaa gcugcccaag     3600

uacagccugu ucgagcucga gaacgggcgg aagcgcaugc uggccuccgc cggcgagcug     3660

cagaagggga acgagcucgc ccugcccagc aaguacguga acuuccugua ccucgccucc     3720

cacuacgaga agcugaaggg cagcccggag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccucga cgagaucauc gagcagaucu ccgaguucag caagcggguc     3840

auccuggcgg acgccaaccu ggacaaggug cucuccgccu acaacaagca ccgcgacaag     3900

cccauccggg agcaggccga gaacaucauc caccuguuca cccugaccaa ccucggcgcc     3960

cccgcggccu ucaaguacuu cgacaccacg aucgaccgca agcgguacac cagcaccaag     4020

gaggugcugg acgccacccu gauccaccag uccaucacgg ggcucuacga gacccgcauc     4080

gaccugagcc agcugggcgg ggacuga                                         4107


<210>  1948
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1948
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cuccucuucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcaucugc      240

uaccugcagg agaucuucuc caacgagaug gccaaggucg acgacagcuu cuuccaccgg      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcgccaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccggaag      420

aagcucgcgg acagcaccga caaggccgac cugcgccuga ucuaccucgc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

guggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgcga gccgcgucga cgccaaggcc auccuguccg cccggcugag caagucccgc      660

cggcucgaga accugaucgc ccagcugccg ggcgagaagc gcaacgggcu guucggcaac      720

cugaucgcgc ugagccucgg gcugacgccc aacuucaagu ccaacuucga ccuggccgag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgcggcca agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg ggugaacucc gagaucacca aggccccgcu gagcgccagc      960

augaucaagc gcuacgacga gcaccaccag gaccucaccc ugcugaaggc gcucgugcgg     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc aguccaagaa cggcuacgcc     1080

ggguacaucg acggcggggc cagccaggag gaguucuaca aguucaucaa gccgauccug     1140

gagaagaugg acggcacgga ggagcuccug gccaagcuga accgcgagga ccugcuccgc     1200

aagcagcgga ccuucgacaa cggcuccauc ccccaccaga uccaccuggg ggagcugcac     1260

gccauccucc gccggcagga ggacuucuac cccuuccuga aggacaaccg cgagaagauc     1320

gagaagaucc ugaccuuccg gauccccuac uacgucggcc cgcucgcgcg cgggaacagc     1380

cgguucgccu ggaugacccg caaguccgag gagacgauca cccccuggaa cuucgaggag     1440

gugguggaca agggcgccag cgcccagucc uucaucgagc ggaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu ccugcccaag cacagccucc uguacgagua cuucaccgug     1560

uacaacgagc ugacgaaggu gaaguacguc accgagggga ugcgcaagcc ggaguuccuc     1620

uccggcgagc agaagaaggc gaucguggac cugcuguuca agaccaaccg gaaggugacc     1680

gucaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacag cguggagauc     1740

uccggcgugg aggaccgcuu caacgccagc cuggggacgu accacgaccu gcucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucguc     1860

cucacccuga cccuguucga ggaccgggag augaucgagg agcgccucaa gaccuacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc gccgguacac gggcuggggg     1980

cgccucuccc ggaagcugau caacggcauc cgcgacaagc agagcgggaa gaccauccug     2040

gacuuccuca aguccgacgg cuucgccaac cgcaacuuca ugcagcugau ccacgacgac     2100

agccugaccu ucaaggagga cauccagaag gcccaggucu ccggccaggg ggacagccuc     2160

cacgagcaca ucgcgaaccu ggccggcucc cccgccauca agaaggggau ccugcagacc     2220

gugaaggugg ucgacgagcu cgugaaggug augggccggc acaagcccga gaacaucguc     2280

aucgagaugg cccgcgagaa ccagacgacc cagaaggggc agaagaacag ccgggagcgc     2340

augaagcgga ucgaggaggg caucaaggag cugggcagcg acauccugaa ggaguacccc     2400

guggagaaca cccagcucca gaacgagaag cuguaccugu acuaccucca gaacgggcgc     2460

gacauguacg uggaccagga gcuggacauc aaccggcugu ccgacuacga cgucgaccac     2520

aucgugccgc agagcuucau caaggacgac uccaucgaca acaaggugcu cacccgcagc     2580

gacaagaacc ggggcaaguc cgacaacguc cccagcgagg agguggugaa gaagaugaag     2640

aacuacugga agcagcugcu gaacgccaag cucaucacgc agcgcaaguu cgacaaccug     2700

accaaggcgg agcgcggggg ccuguccgag cucgacaagg ccggcuucau caagcggcag     2760

cuggucgaga cccggcagau caccaagcac guggcccaga uccuggacag ccgcaugaac     2820

accaaguacg acgagaacga caagcucauc cgggagguga aggucaucac gcugaagucc     2880

aagcugguga gcgacuuccg caaggacuuc caguucuaca aggugcggga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccucaac gcggucgugg ggaccgcccu gaucaagaag     3000

uaccccaagc uggaguccga guucguguac ggcgacuaca aggucuacga cgugcgcaag     3060

augaucgcca agagcgagca ggagaucggg aaggccaccg cgaaguacuu cuucuacucc     3120

aacaucauga acuucuucaa gaccgagauc acgcucgcca acggcgagau ccggaagcgc     3180

ccccugaucg agaccaacgg ggagaccggc gagaucgugu gggacaaggg ccgggacuuc     3240

gccaccgucc gcaaggugcu gagcaugccc caggugaaca ucgucaagaa gacggaggug     3300

cagaccgggg gcuucuccaa ggagagcauc cuccccaagc gcaacuccga caagcugauc     3360

gcccggaaga aggacuggga ccccaagaag uacgggggcu ucgacagccc caccguggcc     3420

uacagcgucc uggugguggc gaaggucgag aaggggaagu ccaagaagcu caagagcgug     3480

aaggagcugc ugggcaucac caucauggag cgcuccagcu ucgagaagaa cccgaucgac     3540

uuccucgagg ccaagggcua caaggaggug cggaaggacc ugaucaucaa gcugcccaag     3600

uacucccucu ucgagcugga gaacgggcgc aagcggaugc uggccagcgc cggcgagcuc     3660

cagaagggga acgagcuggc ccugcccucc aaguacguca acuuccucua ccuggcgagc     3720

cacuacgaga agcugaaggg cuccccggag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccugga cgagaucauc gagcagauca gcgaguucuc caagcgcgug     3840

auccuggccg acgccaaccu cgacaagguc cugagcgccu acaacaagca ccgggacaag     3900

cccauccgcg agcaggccga gaacaucauc caccuguuca cgcucaccaa cuucggggcg     3960

cccgccgccu ucaucuacuu cgacaccacc aucggccgga accgcuacaa guccaucaag     4020

gaggugcugg acgccacgcu gauccaccag agcaucaccg gccucuacga gacccggauc     4080

gaccuguccc agcucggggg cgacuga                                         4107


<210>  1949
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1949
augaaguacg agagcgagga guacuacauc ggccuggaca uggggaccuc cagcgugggc       60

ugggccguca ccgaccccca cuaccaccuc auccgcaaga aggggaagga ccuguggggc      120

gcccggcugu ucgagaaggc gaacacggcc caggagcgcc ggaccaaccg caucaaccgg      180

cgccggaacc agcgccggaa gcagcgcauc gccuucaucc gggaggccuu ccgcaaggag      240

guggagaagg uggacgucaa cuucuuccgg cgccucgacg agucccagcu guaccuggag      300

gacaagagca ucaaggagcc guaccuccug uucaacgacc ugaacuacac cgacaaggac      360

uucuaccacc aguaccccac cguguuccac cucaucacgg accugcugac cuccgaccgg      420

aaguucgaca uccgcgagau cuacaucgcc gugagcuccc ucuacaacca ccgggggaac      480

uuccugaaca agagccuggg caacgagacc caggacgagg accucgucuu caagaaccug      540

gacgaggcgu ucuccgccug cggcaugaac uggaacacca aggccgagaa caucgaggug      600

gugaagcaga uccugugcga ccgcagccuc aacaagaagg agaagcagga ccagcuggcc      660

cacugccugg agaucaacaa gaagacggag aaggagaaga ucgaguuccu caagcugcug      720

accgggggca aggcggacuc caagaagaug uucccccagu accacuacga gcgggacgag      780

aagcucagcu ucgccuucgc cgacgagaac ggggacgaga acugggagga gacccugaac      840

gccaucgagg agcgccugac cuccgacgag uacggccuca uccuggcccu caagagcguc      900

uacgacuggu ccauccucgc ggaccugaug aagaacgaga agacggggga gaccuacccc      960

uaccugagca uggcccggaa ggacuccuac gagaagcaca aggccgaccu ccgccugcuc     1020

aagagcguga ucaagcgccu caucccgaac gaguacaacc acuucuuccg gcaguacgac     1080

ggcaagggca agaccuacac caacuacauc ggguccgugc aguaccacga gaagcgccac     1140

cgguucaagc acaucacgac caaggaguuc uacgacggcc ugaagaagcu gcucaagccc     1200

uacagcgagg acgaggagau caagcagauc cugcaggaca ucgagagcga gaccuuccug     1260

cccaagcaga ucaccaacau gaacgggguc auccccaacc aggugcacgc caaggagcuc     1320

aaggugaucc uggagaaggc cucccagcac uacgacuucc ugaacgagac gaacgagagc     1380

ggccucaccg ucaaggagca gaucaugcag auguucaccu uccagauccc guacuacgug     1440

ggcccccugg cgaaccugaa cgggaagggc aacggguggg ccgugcgcua cgucggcaag     1500

gagcggacca agguguaccc cuggaacaug gacgagguga ucgacgagaa ggagacggcc     1560

gaccaguuca ucaagcgcau gaucuccacg ugcaccuacc ucggggacga guacguccug     1620

cccaagagcu ccauccugua cgagaaguuc gccgugcuga acgagcucaa cagccugaag     1680

aucaacggcc agccgguguc cgucgaccug aagcagaaga ucuaccggga ccucuucacc     1740

aagggcaagc gcgugaccca gaaggggcug cugcucuacc ugaagcggga gggcgucauc     1800

agcaaguccg aggagaccau ccugagcggg uucgacaucg acuucaagaa cacccucucc     1860

agcuaccgca aguucugcga gguguucgcc accaacgagc ugaacgacga gcagauccgg     1920

auggcggagg acaucaucga guggcugacg guguucggcg acuccaagaa guucguccgc     1980

gagaagaucc acgggaacua cccccagauc accgccaacc agcucaagcg gauccugagc     2040

uucaacuuca aggacugggg caagcugucc aagcaguucc ucgagcugga gggcgaggac     2100

cgcgagaccg gcaagagcug cucccugauc caggcccucu gggacaccaa caacaaccug     2160

aaccagcugc ucagcucccg guucacguac aaggagaacc uggagaagaa gcgcaagaag     2220

aucgagaugg accuguucga guucacccac gaggaccucg acgaccugua caucagcgcc     2280

cccgugaagc ggaugaucug gcaggcccug aagaucauga aggacaucgu gaucgucaug     2340

aaggagagcc ccaccaagcu guucaucgag augacccgca acaacgagaa cgagaagacc     2400

cggacggugu cccgcaagaa caagcuggug gaccuguaca agaagaucaa gggggagagc     2460

cgcgacugga ucaaggagcu cgagcgguac gaggagaagg acuuccgcuc caagaagcug     2520

uaccuguacu acacccagau gggcaagagc gcguacuccg gggagccgau cgacauggac     2580

aagcuguucg acaagaacgu cuacgacauc gaccacaucu accccaagag caaggugaag     2640

gacgacuccc ugcugaacaa cugcgugcuc gucaagcgga aggagaacau ggacaagggg     2700

aacaacuucc ccaucgaccc cgacauccag accaagcagg agcaccugug gaaguucugg     2760

cuggagaagg gccucaucac ccaggagaag uucaaccgcc ugacgcggaa gaagccguuc     2820

accaacgacg agcuggccga cuucgugaac cgccagcucg uggagaccgg ccaggccacc     2880

aagacgaucg ccgaccuguu caagaugacc uuccccaaca gcgacaucgu cuacgugaag     2940

gcggggaacg ugaccgacuu ccggcaguac aucgaggucu acaagugccg cgaggugaac     3000

aaccugcacc acgccuacga cgccuaccuc aacguggucg ugggcaacgu guacgacacc     3060

aaguucacga agaaccccau gaacuacauc aaggagcagc ggaaggagaa cuccaacgag     3120

cgcuaccaca uggacaagau cuucgaguuc cccgucaucc ggaacgggaa ggaggccugg     3180

aucaccaaga aggacgagag caucaacauc gugcgcaaga cccuggcccg gaagaccccg     3240

cuggugacga agcgcaacuu cgaggcgaac ggcaccaucg ccaaggccac caucuacccc     3300

aagggcaacg ucaaggacca gggguaccuc ccccggaaga ccuccauccc cgcccugcgc     3360

gacaugaaga aguacggcgg ggugacgagc auccagaucg ccuacuucuu ccugguggag     3420

cacgagguca agaagaagcg gauccgcacc aucgagugcg ugccgaucua ccggaaggag     3480

gagcucaaga ccaaggagca gcuggagcgc uacugccggg aggagcugaa gcucaucaac     3540

cccuccaucc gccuggagaa gaucaagccc uacagccugc ucaagaugaa cggcaacuuc     3600

uucuacauca ccgggaagac gaacgaccgc uucucccugg cgaacgccau ggagcuguac     3660

guggacaagg ucagcgagaa ccacauccgg gaccucguga aguacaagaa cugcggcuac     3720

uucaacgugg acgacaagga ggcccgcaag cggaacgagc gccuguacac cauccugacc     3780

aagaagcacc ucaccgucuu caagggcucc ccgaagccca ugggggagaa gcugcagcag     3840

uaccagcagc uguuccucga gcuggaggug agcaagcagg ucuacgugcu gcuccagauc     3900

cugcagcuga cggccaccaa cgugaagacc aaggccgacc ucaccgcgau cggcgggaag     3960

gccaacgagg gcacgaugga caucagcaag aaccuggaga acgugaucaa gaaccagauc     4020

aagaaggggc ugguccccga gcucaugcug aucaaccagu cccccaccgg ccuguucgag     4080

agcgagaucg accuccugac caucuga                                         4107


<210>  1950
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1950
augagccgcc ccuacaacau cggccuggac aucgggaccu ccagcaucgg cugguccgug       60

gucgacgacc agagcaagcu cguguccgug cgggggaagu acggcuacgg gguccgccug      120

uacgacgagg gccagaccgc cgccgagcgg cgcagcuucc ggacgacccg ccggcgccug      180

aagcggcgca aguggcggcu cggccugcug cgcgagaucu ucgagccgua caucaccccc      240

guggacgaca ccuucuuccu ccggaagaag caguccaacc ugagccccaa ggaccagcgc      300

aagcuguacc cccagacguc ccucuucaac gaccggaccg accgcgcguu cuacgacgac      360

uacccgacca ucuaccaccu ccgguacaag cugaugaccg agaagcgcca guucgacauc      420

cgggagaucu accuggccau gcaccacauc gugaaguacc gcgggcacuu ccugaacgag      480

gcccccguca gcuccuucaa gagcuccgag aucaaccugg uggcccacuu cgaccggcuc      540

aacacgaucu ucgccgaccu guucagcgag uccggcuucc agcuggagac cgacaagcuc      600

gcggagguga aggcccugcu gcucgacaac caccagagcg ccuccaaccg ccagcgccag      660

gcccugcugc ucaucuacac ccccagcacc aacaaggcgg ucgagaagca gaacaaggcc      720

aucgccacgg agcugcugaa ggccauccuc gggcugaagg ccaaguucaa cgugcugacc      780

ggcaucgagg cggaggacgu gaagaccugg acccucacgu ucaacgccga gaacuucgac      840

gaggagaugg ucaagcugga guccagccug gacgacaacg cccaccagau caucgagagc      900

cuccaggagc uguacuccgg ggugcugcuc gccggcaucg ugcccgagaa ccagagccug      960

ucccaggcca ugaucaccaa guacgacgac caccagaagc accugaagau gcucaaggcg     1020

guccgggagg cccuggcccc ggaggaccgc cagcggcuga agcaggccua cgaccaguac     1080

guggacggcc aggagaacac caaggccuac agcaaggagg acuucuacgg ggacaucacc     1140

aaggcgcuca agaacaaccc cgaccacccc aucguguccg agaucaagaa gcugaucgag     1200

cuggaccagu ucaugcccaa gcagcgcacg aaggacaacg gcgccauccc gcaccagcuc     1260

caccagcagg agcuggaccg gaucaucgag aaccagcagc aguacuaccc cuggcuggcc     1320

gagcucaacc ccaacagcaa gcgccagacc gucgccaagu acaagcugga cgagcuggug     1380

gccuuccggg ugcccuacua cgucgggccg cugaucaccg cggagcagca gcagcagucc     1440

agcgacgcca aguucgccug gaugauccgc aaggccgagg gccagaucac ccccuggaac     1500

uucgacgaca agguggaccg gcaggcgucc gccaacgagu ucaucaagcg caugacgacc     1560

accgacaccu accuccuggc cgaggacgug cugcccaagc agagccucau cuaccagcgg     1620

uucgaggucc ugaacgagcu gaacggccuc aagaucgacg accagcccau cacgaccgag     1680

cugaagcagg ccaucuucac cgaccuguuc augcagaaga ccuccgugac ggucaagaac     1740

auccaggacu accucgugag cgagaagcgc uacgccuccc ggccggcgau caccgggcug     1800

agcgacgaga acaaguucaa cucccgccug agcaccuacc acgaccucaa gaccaucgug     1860

ggcgacgccg ucgacgacgu ggacaagcag gccgaccugg agaagugcau cgaguggucc     1920

acgaucuucg aggacgggaa gaucuacagc gccaagcuga acgagaucga cuggcucacc     1980

gaccagcagc gggugcagcu ggccgcgaag cgcuaccggg gcugggggcg ccuguccgcc     2040

aagcuccuga cccagaucgu caacgccaac ggccagcgga ucauggaccu gcucugggac     2100

accaccgaca acuucaugcg caucgugcac agcgaggacu ucgacaagcu gaucacggag     2160

gccaaccaga ugaugcuggc cgagaacgac gugcaggacg ucaucaacga ccucuacacc     2220

agcccccaga acaagaaggc gcugcgccag auccugcucg uggugaacga cauccagaag     2280

gccaugaagg gccaggcccc cgagcggauc cugaucgagu ucgcccgcga ggacgagguc     2340

aacccccggc uguccgugca gcgcaagcgc cagguggagc aggucuacca gaacaucagc     2400

aacgagcucc ugaacaacac cgagauccgg aacgagcuga aggaccucuc caacagcgcg     2460

cuguccaaca cccggcuguu ccucuacuuc augcagggcg ggcgcgacau guacacgggg     2520

gacagccuga acaucgaccg gcuguccacc uacgacaucg accacauccu cccgcagagc     2580

uucaucaagg acaacucccu ggacaaccgc gugcugguga gccagcggau gaaccgcucc     2640

aaggccgacc agguccccac cgacuucacc agcguggagc ucggccagaa gaugcagauc     2700

cagugggagc agaugcugcg ggccgggcug aucacgaaga agaaguacga caaccucacc     2760

cugaaccccg accacaucuc caaguacgcc augaagggcu ucaucaaccg ccagcuggug     2820

gagacccggc aggucaucaa gcucgccacc aaccugcuga uggagcagua cggcgaggac     2880

aacaucgagc ucaucacggu gaaguccggg cugacccacc agaugcgcac cgaguucgac     2940

uuccccaaga accggaaccu gaacaaccac caccacgcgu ucgacgccua ccucaccgcc     3000

uucgugggcc uguaccugcu caagcgcuac ccgaagcuga agcccuacuu cgucuacggg     3060

gaguaccaga aggccuccca gcaggacaag uggcggaacu ucaacuuccu gaacggccuc     3120

aagaaggacg agcuggugga cgagaacacg gaggccguga ucuggaacaa ggagagcggg     3180

cuggcguacc ucaacaagau cuaccaguuc aagaagaucc uggucacccg cgaggugcac     3240

gagaacuccg gcgcccuguu caaccagacc cucuacgccg ccaaggacga caaggccagc     3300

ggccagggcg ggaagcagcu gauccccgcg aagcaggacc ggccgaccgc ccuguacggg     3360

ggcuacagcg gcaagacggu ggccuacaug ugcaucgucc gcaucaagaa caagaagggg     3420

gaccucuaca aggugugcgg cguggagacc uccuggcugg cccagcugaa gcagcucacc     3480

gacgaggaca gcaagaaggc guuccugaag cagaagauca gcccccaguu caccaagguc     3540

aagaagcaga aggggacgau cgugaaggug gucgaggacu ucgaggugau cgcgccccac     3600

auccugauca accagcgcuu cuucgacaac ggccaggagc ucacccuggg gagcgccacc     3660

uacaagcaca acgagcagga gcugauccuc gacaagaccg ccgugaagcu gcugaacggc     3720

gcccuccccc ugacgcaguc cgaggagcug gccgagcagg ucuacgacga gauccucgac     3780

caggugaugc acuacuuccc gcuguacgac accaaccagu uccgggccaa gcugagcgcc     3840

ggcaaggccg ccuucaugaa gcuccccugg aagucccagu gggacgggaa caagauggug     3900

caggucggcc agcaggucau ccuggaccgc gugcugaucg ggcuccacgc gaacgccgcc     3960

gugagcgacc ugggcgugcu gaagaucucc acgccccucg ggaagaugca gcagcccagc     4020

ggcaucuccc ugagcccgga cacccagauc aucuaccagu cccccaccgg ccuguucgag     4080

cggcgcgugg cccugcggga ccucuga                                         4107


<210>  1951
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1951
augagccgcc ccuacaacau cggccuggac aucgggaccu ccagcaucgg cugguccgug       60

gucgacgacc agagcaagcu cguguccgug cgggggaagu acggcuacgg gguccgccug      120

uacgacgagg gccagaccgc cgccgagcgg cgcagcuucc ggacgacccg ccggcgccug      180

aagcggcgca aguggcggcu cggccugcug cgcgagaucu ucgagccgua caucaccccc      240

guggacgaca ccuucuuccu ccggcagaag caguccaacc ugagccccaa ggaccagcgc      300

aagcuguacc cccagacguc ccucuucaac gaccggaccg accgcgcguu cuacgacgac      360

uacccgacca ucuaccaccu ccgguacaag cugaugaccg agaagcgcca guucgacauc      420

cgggagaucu accuggccau gcaccacauc gugaaguacc gcgggcacuu ccugaacgag      480

gcccccguca gcuccuucaa gagcuccgag aucaaccugg uggcccacuu cgaccggcuc      540

aacacgaucu ucgccgaccu guucagcgag uccggcuucc agcuggagac cgacaagcuc      600

gcggagguga aggcccugcu gcucgacaac cagcagagcg ccuccaaccg ccagcgccag      660

gcccugagcc ugaucuacac ccccuccacc aacaaggcgg ucgagaagca gaacaaggcc      720

aucgccacgg agcuccugaa ggccauccug gggcucaagg ccaaguucaa cgugcugacc      780

cggaucgagg cggaggacgu gaaggccugg acccugaccu ucaacgccga gaacuucgac      840

gaggagaugg ucaagcucga gagcagccug gacgacaacg cccaccagau caucgagucc      900

cugcaggagc ucuacagcgg cauccugcug gccgggaucg ugcccgagaa ccagucccuc      960

agccaggcga ugaucaugaa guacgacgac caccagaagc accugaagau gcugaaggcc     1020

gugcgcgagg cccucgcccc ggaggaccgg cagcgccuga agcaggccua cgaccaguac     1080

gucgacggcc aggagaacac gaaggcguac uccaaggagg acuucuacgg cgacaucacc     1140

aaggcccuga agaacaaccc cgaccacccc aucgugagcg agaucaagaa gcucaucgag     1200

cuggaccagu ucaugcccaa gcagcggacc aaggacaacg gggccauccc gcaccagcug     1260

caccagcagg agcucgaccg caucaucgag aaccagcagc aguacuaccc cuggcuggcc     1320

gagcugaacc ccaacuccaa gcggcagacc guggccaagu acaagcucga cgagcugguc     1380

gcguuccgcg ugcccuacua cgugggcccg cugaucacgg ccgagcagca gcggcagagc     1440

uccgacgcca aguucgccug gcugauccgc aaggcggagg ggcggaucac ccccuggaac     1500

uucgacgaca aggucgaccg ccaggccagc gccaacgagu ucaucaagcg gaugaccacc     1560

acggacaccu accuccuggc cgaggacgug cugcccaagc agucccucau cuaccagcgc     1620

uucgaggugc ugaacgagcu gaacggccuc aagaucgacg accagcccau caccaccgag     1680

cugaagcagg ccaucuucac ggaccuguuc augcagaaga ccagcgucuc cgugaagaac     1740

auccaggacu accucgucag cgagaagcgg uacgcguccc gcccggccau caccggccug     1800

agcgacgaga acaaguucaa cucccggcug agcaccuacc acgaccucaa ggccaucgug     1860

ggggacgccg uggaggacgu cgacaagcag gucgaccugg agaagugcgu ggaguggucc     1920

acgaucuucg aggacggcaa gaucuacagc gccaagcuga acgagaucga cuggcucacc     1980

gaccagcagc gcgugcagcu ggcggccaag cgguaccgcg gguggggccg ccugagcgcc     2040

aagcuccuga cccagaucgu gaacgccaac gggcagcgga ucauggaccu gcucugggac     2100

accacggaca acuucaugcg caucgugcac uccgaggacu ucgacaagcu gaucaccgag     2160

gccaaccaga ugaugcuggc ggagaacgac guccaggacg ugaucaacga ccucuacacc     2220

agcccccaga acaagaaggc ccugcggcag auccugcucg uggucaacga cauccagaag     2280

gccaugaagg gccaggcccc cgagcgcauc cugaucgagu ucgcgcggga ggacgaggug     2340

aacccccgcc uguccgugca gcggaagcgc caggucgagc aggucuacca gaacaucagc     2400

aacgagcucc ugaacaacac cgagauccgg aacgagcuga aggaccucuc caacagcgcc     2460

cuguccaaca cccgccuguu ccucuacuuc augcagggcg ggcgggacau guacacgggc     2520

gacagccuga acaucgaccg ccuguccacc uacgacaucg accacauccu cccgcagagc     2580

uucaucaagg acaacucccu ggacaaccgg gugcugguga gccagaagau gaaccgcucc     2640

aaggccgacc aggugcccac cgacuucacc agcguggagc ucgggaagaa gaugcagcug     2700

cagugggagc agaugcugcg ggccggccuc aucacgaaga agaaguacga caaccugacc     2760

cugaaccccg accacaucuc caaguacgcc augaaggggu ucaucaaccg ccagcucguc     2820

gagacccgcc aggugaucaa gcuggcgacc aaccugcuca uggagcagua cggcgaggac     2880

aacaucgagc ugaucacggu gaaguccggc cugacccacc agaugcggac cgaguucgac     2940

uuccccaaga accggaaccu caacaaccac caccacgccu ucgacgccua ccugaccgcc     3000

uucgucgggc uguaccuccu gaagcgcuac ccgaagcuga agcccuacuu cguguacggc     3060

gaguaccaga aggccuccca gcaggacaag uggcggaacu ucaacuuccu caacgggcug     3120

aagaaggacg agcuggugga cgagaacacg gaggcgguca ucugggacaa ggagagcggc     3180

cucgccuacc ugaacaagau cuaccaguuc aagaagaucc uggugacccg cgaggugcac     3240

gagaacagcg gggcccucuu caaccagacc cuguacgccg ccaaggacga caaggcgucc     3300

ggccagggcg ggaagcagcu gauccccgcc aagcaggacc gcccgaccgc ccucuacggc     3360

ggguacagcg gcaagacggu cgccuacaug ugcaucgugc ggaucaagaa caagaagggc     3420

gaccuguaca aggugugcgg ggucgagacc uccuggcugg cgcagcucaa gcagcugacc     3480

gacgaggaca gccagaaggc cuuccugaag cagaagauca gcccccaguu caccaaggug     3540

aagaagcaga agggcacgau cgugaagguc guggaggacu ucaaggugau cgcgccccac     3600

auccucauca accagcgcuu cuucgacaac gggcaggagc ugacccuggg cagcgccacc     3660

uacaagcaca acgagcagga gcucauccug gacaagaccg ccgucaagcu gcucaacggg     3720

gcccugcccc ugacgcaguc cgaggagcuc gccgagcagg ucuacgacga gauccuggac     3780

caggugaugc acuacuuccc gcuguacgac accaaccagu uccgggccaa gcucagcgcc     3840

ggcaaggccg cguucaugaa gcugcccugg aaguccaucu gggacggcaa caagaugacc     3900

cagaucgggc agcagaucau ccuggaccgc auccucaucg gccugcacgc caacgccgcc     3960

gugagcgacc ugggggugcu caagaucucc acgccccugg gcaagaugca gcagcccagc     4020

gggaucuccc ugagcccgga cacccagauc aucuaccagu cccccaccgg ccuguucgag     4080

cggcgcgugg cccuccggga ccuguga                                         4107


<210>  1952
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1952
augguggaga agaaguccua cagcaucggc cuggacaucg ggaccaacag cgucggcugg       60

gccgugauca ccgacgacua caaggugccc gccaagaaga ugaagguccu cgggaacacg      120

ucccgccaga gcaucaagaa gaacaugauc ggcgcgcugc uguucgacga gggcgggccg      180

gccgccucca cccgggugaa gcgcaccacc cggcgccggu acacgcgccg gaagaaccgc      240

cucugcuacc ugcgggacau cuucgagagc gagaugcaca ccaucgacaa gcacuucuuc      300

cugcgccucg aggacuccuu ccugcacaag agcgacaagc gguacgaggc ccaccccauc      360

uucggcaccc ugcaggagga gaaggccuac cacgacaacu accccaccau cuaccaccuc      420

cgcaaggcgc uggccgacaa cacggagaag gccgaccugc ggcucaucua ccuggcccug      480

gcgcacauca ucaaguuccg cgggcacuuc cucaucgagg gcgcccuguc cgccaacaac      540

accgacgugc agcagcuggu ccacgcccuc guggacgccu acaacaucau guucgaggag      600

gaccagcugg acaucgaggc gaucgacgug aaggccaucc ugaccgagaa gaucagcaag      660

acccggcgcc ucgagaaccu gaucuccaac auccccgggc agaagaagaa cggccuguuc      720

gggaaccuca ucgcccugag ccugggccuc acgccgaacu ucaaguccca cuucaaccug      780

cccgaggacg ccaagcugca gcucgccaag gacaccuacg acgaggagcu gaacaaccug      840

cucacccaga ucggcgacga guacgcggac cuguuccuga gcgccaagaa ccucuccgac      900

gccauccugc ugagcgacau ccucaccguc aacggggacg gcacgcaggc gccccugucc      960

gccagccuga ucaagcggua cgaggagcac cgccaggacc ucgcccugcu gaagcagaug     1020

uucaaggagc agcuccccga ccuguaccgg gacguguuca ccgacgagaa caaggacggg     1080

uacgccggcu acaucuccgg caagaccagc caggaggccu ucuacaagua caucaagccg     1140

auccuggaga cccucgacgg ggccgaggac uuccugacga agaucaaccg cgaggacuuc     1200

cugcggaagc agcgcaccuu cgacaacggc agcauccccc accagaucca ccugggggag     1260

cuccaggcca uccuggagcg ccagcaggcg uacuaccccu uccugaagga caaccaggag     1320

aagaucgaga agauccucac cuuccggauc cccuacuaca ucggcccgcu ggcccgcggg     1380

aacucccggu ucgccuggcu gacccgcacg agcgaccaga agaucacccc cuggaacuuc     1440

gacgagaugg uggaccagga ggccuccgcg caggccuuca ucgagcggau gaccaacuuc     1500

gacgaguacc ucccccagga gaagguccug cccaagcaca gccugaccua cgaguacuuc     1560

acgguguaca acgagcucac caaggugaag uacgucaccg agggcaugac caagccggag     1620

uuccuguccg ccggccagaa ggagcagauc guggagcucc uguucaagaa guaccgcaag     1680

gugacgguca agcagcugaa ggaggacuuc uucagcaaga ucgagugcuu cgacaccgug     1740

gacaucuccg ggguggagga caaguucaac gccagccugg gcaccuacca cgaccuccug     1800

aagaucauca aggacaaggc cuuccuggac aacuccgaga acgagaacau caucgaggac     1860

aucauccuca cccugacgcu guucgaggac aaggagauga ucgcgaaccg gcucgccguc     1920

uacgaggacc uguucgacca gaacgugcug aagcagcuca agcgccggca cuacaccggg     1980

uggggccgcc ugagcaagca gcugaucaac gggaugcggg acaagcacac cggcaagacc     2040

auccucgacu uccugaaggc cgacggcuuc aucaaccgca acuucaugca gcugaucaac     2100

gacgacaacc ucuccuucaa ggaggagauc aagaaggccc aggagggcgg gcugaaggac     2160

agcaucaacg accagauccg ggaccuggcc gggucccccg cgaucaagaa gggcauccuc     2220

cagaccauca acaucgugga cgagaucguc aagaucaugg ggaaggcgcc ccagcacauc     2280

guggucgaga uggcccgcga cgugcagaag acggacaucg gcgugaagca gagccgggag     2340

cgcaugaagc ggguccagga ggugcugaag aagcugggcu cccagcuccu gaaggagcac     2400

cccguggaga acuuccagcu gcagaacgag cgccucuacc uguacuaccu gcagaacggg     2460

aaggacaugu acaccggcga ggagcucagc aucuccaacc ugagccacua cgacaucgac     2520

cacaucaucc cgcgguccuu caucaaggac gacagcaucg acaacaaggu ccugacccgc     2580

agcgagcaca accgcgggaa gaccgacaac gugcccucca ucgagguggu caagcggaug     2640

aagcccuacu ggcagaagcu ccuggacacg aaggugauca gccagcgcaa guucgacaac     2700

cugaccaagg ccgagcgggg cggccuccag gaguccgaca aggccaacuu cauccagcgc     2760

cagcuggugg agacccggca gaucaccaag cacgucgccc agauccugga cagccgcuuc     2820

aacacggagc gggacgagaa ggaccgcccc auccggcgcg ugaaggugau cacccucaag     2880

uccaaguucg ucagcgacuu ccggcaggac uucggguucu acaagcugcg cgagaucaac     2940

gacuaccacc acgcccacga cgccuaccug aacgccgugg ugggcaccgc gcuccugaag     3000

auguacccga agcuggccuc cgaguucguc uacggggacu accagaagua cgaccucaag     3060

cggauggugg gcaagagcgg gaaggccucc ggccacgcca ccgccaagua cuucuucuac     3120

agcaaccuga ugaacuucuu caaguccgag gugaagcugg cgaacggcaa caucaucaag     3180

cgcagcccca ucgaggucaa cgaggagacg ggggagaucg ugugggacaa gaccaaggac     3240

uucggcaccg ugcggaaggu ccucuccgcg ccccagguga acaucgugaa gaagaccgag     3300

auccagaccg ggggcuucag caacgagacg auccugucca agggcaagag cuccaagcug     3360

aucccccgca agaacaagug gcgggacacc accaaguacg ggggcuucaa cacgccgacc     3420

gucgccuaca gcgugcucgu ggucgccaag guggagaagg ggaaggccaa gaagcugaag     3480

cccgugaagg agcuggucgg caucaccauc auggagcgca ccaaguucga ggccaacccc     3540

aucgccuucc ucgagagcaa gggguaccac gacauccagg agcaccugau gaucacgcug     3600

ccgaaguacu cccucuucga gcuggagaac ggccggcgcc ggcugcucgc cagcgccacc     3660

gagcugcaga aggggaacga gauggugcug ccccagcacc ucgugaccuu ccuguaccgc     3720

gucuccaagc gcgacaaggg cacccagagc gagaacaugg aguacaucuc caaccacaag     3780

gagaaguuca ucgagaucuu ccacuacauc auccgguacg ccgagaagaa cgugaucaag     3840

cccaagguga ucgagcgccu gaacgacacg uucaaccaga aguucaacga cagcgaccuc     3900

accgagcugu ccaucagcuu ccugaaccuc uucaaguuca ccuccuucgg cgcgcccgag     3960

aaguucaccu uccugaacag cgagaucaag caggacgacg uccgguaccg cuccacgaag     4020

gagugccuga acagcacccu cauccaccag uccgugaccg ggcuguacga gacccggauc     4080

gaccugagcc aguucggcgg ggacuga                                         4107


<210>  1953
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1953
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcaucugc      240

uaccugcagg agaucuucuc caacgagauc gccaaggucg acgacagcuu cuuccaccgg      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcgccaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccggaag      420

aagcucgcgg acagcaccga caaggccgac cugcgccuga ucuaccucgc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

guggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgcga gccgcgucga cgccaaggcc auccuguccg cccggcugag caagucccgc      660

cggcucgaga accugaucgc ccagcugccg ggcgagaagc gcaacggccu cuucgggaac      720

cugaucgcgc ugagccucgg gcugacgccc aacuucaagu ccaacuucga ccuggccgag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgcggcca agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg ggugaacacc gagaucacca aggccccgcu guccgccagc      960

augaucaagc gcuacgacga gcaccaccag gaccucacgc ugcugaaggc gcucgugcgg     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc agagcaagaa cggcuacgcc     1080

ggguacaucg acggcggggc cucccaggag gaguucuaca aguucaucaa gccgauccug     1140

gagaagaugg acggcaccga ggagcuccug gccaagcuga accgcgagga ccugcuccgc     1200

aagcagcgga ccuucgacaa cggcagcauc ccccaccaga uccaccuggg ggagcugcac     1260

gccauccucc gccggcagga ggacuucuac cccuuccuga aggacaaccg cgagaagauc     1320

gagaagaucc ugaccuuccg gauccccuac uacgucggcc cgcucgcgcg cgggaacucc     1380

cgguucgccu ggaugacgcg caagagcgag gagaccauca cccccuggaa cuucgaggag     1440

gugguggaca agggcgccuc cgcccagagc uucaucgagc ggaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu ccugcccaag cacucccucc uguacgagua cuucacggug     1560

uacaacgagc ugaccaaggu gaaguacguc accgagggga ugcgcaagcc ggcguuccuc     1620

agcggcgagc agaagaaggc caucguggac cugcuguuca agaccaaccg gaaggugacg     1680

gucaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacuc cguggagauc     1740

agcggcgugg aggaccgcuu caacgccucc cuggggaccu accacgaccu gcucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucguc     1860

cucacccuga cccuguucga ggaccgggag augaucgagg agcgccucaa gacguacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc gccgguacac cggcuggggg     1980

cgccucagcc ggaagcugau caacggcauc cgcgacaagc aguccgggaa gaccauccug     2040

gacuuccuca agagcgacgg cuucgccaac cgcaacuuca ugcagcugau ccacgacgac     2100

ucccugaccu ucaaggagga ccuccagaag gcgcagguca gcggccaggg ggacucccug     2160

cacgagcaca ucgccaaccu ggccggcagc cccgccauca agaaggggau ccuccagacg     2220

gugaaggugg ucgacgagcu ggugaaggug augggccggc acaagcccga gaacaucguc     2280

aucgagaugg cccgcgagaa ccagaccacc cagaaggggc agaagaacuc ccgggagcgc     2340

augaagcgga ucgaggaggg caucaaggag cugggcagcg acauccucaa ggaguacccc     2400

guggagacca cgcagcugca gaacgagaag cuguaccucu acuaccugca gaacgggcgc     2460

gacauguacg uggaccagga gcuggacauc aaccggcuca gcgacuacga cgucgaccac     2520

aucgugccgc aguccuuccu gaaggacgac agcaucgaca acaaggugcu gacccgcucc     2580

gacaagaacc ggggcaagag cgacaacguc cccuccgagg agguggugaa gaagaugaag     2640

aacuacugga agcagcuccu gaacgcgaag cugaucaccc agcgcaaguu cgacaaccuc     2700

accaaggccg agcgcggggg ccugagcgag cuggacaagg ccggcuucau caagcggcag     2760

cucgucgaga cccggcagau cacgaagcac guggcccaga uccuggacuc ccgcaugaac     2820

accaaguacg acgagaacga caagcugauc cgggaggugc gcgucaucac ccucaagagc     2880

aagcuggugu ccgacuuccg gaaggacuuc caguucuaca aggugcgcga gaucaacaac     2940

uaccaccacg cccacgacgc guaccugaac gccgucgugg ggaccgcccu caucaagaag     3000

uaccccaagc uggagagcga guucguguac ggcgacuaca aggucuacga cauccggaag     3060

augaucgcca aguccgagca ggagaucggg aaggcgacgg ccaaguacuu cuucuacagc     3120

aacaucauga acuucuucaa gaccgagauc acccuggcca acggcgagau ccgcaagcgg     3180

ccccucaucg agaccaacgg ggagacgggc gagaucgugu gggacaaggg ccgcgacuuc     3240

gccaccgugc gcaagguccu guccaugccc caggugaaca ucgugaagaa gaccgagguc     3300

cagaccgggg gcuucagcaa ggaguccauc cugcccaagc ggaacagcga caagcucauc     3360

gcccgcaaga aggacuggga ccccaagaag uacgggggcu ucgacucccc cacgguggcg     3420

uacagcgugc uggucguggc caagguggag aagggcaaga gcaagaagcu gaaguccguc     3480

aaggagcucg uggggaucac caucauggag cggagcuccu ucgagaagga cccgguggac     3540

uuccuggagg ccaagggcua caaggagguc cgcaaggacc ugaucaucaa gcuccccaag     3600

uacagccugu ucgagcugga gaacgggcgg aagcgcaugc ucgccuccgc cggcgagcug     3660

cagaagggga acgagcuggc gcuccccagc aaguacguga acuuccugua ccuggccucc     3720

cacuacgaga agcucaaggg cagcccggag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccugga cgagaucauc gagcagaucu ccgaguucag caagcggguc     3840

auccucgccg acgccaaccu ggacaaggug cuguccgccu acaacaagca ccgcgacaag     3900

cccauccggg agcaggcgga gaacaucauc caccucuuca cccugaccaa ccugggggcc     3960

cccgccgccu ucaagugcuu cgacacgacc aucggccgca accgguacaa gagcaucaag     4020

gaggugcucg acgcgacccu gauccaccag uccaucaccg gccuguacga gacgcgcauc     4080

gaccucagcc agcucggggg cgacuga                                         4107


<210>  1954
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1954
auggacaaga aguacagcau cggccuggac aucgggaccu ccagcaucgg cuucgugggg       60

gucgacgaga acggcuggcc ccucaacauc aaggggaagc gcgccaucgg cgcccaccug      120

uuccgggagg gcgaggcggc cgccgagacc cgcggguucc ggacgacccg ccggcgcuac      180

aaccggcgca aguggcggcu gcgccuccug cgggagaucu ucgacgagcc gaucucccgc      240

guggacccca ccuucuucgc ccggaugaag gagagcuccg ugagccccaa ggaccccaac      300

uacgacggcc uggccaagga gauccucauc ccgcgcgaca aggacgggaa gauccuguac      360

cccaccaucu accaccuccg gcacgcgcug auguacgagg accaccaguu cgacauccgc      420

gagaucuacc uggccaucca ccacaucguc aaguaccggg gccacuuccu gcucgacggg      480

gccgccaaga acuucgaguc cagcaaccug gaccugaagu ccgacaucca cgagaucaac      540

gcgauccucg ugggcaucuu cggcaacgag cagguccagu ucaacgacca guacguggag      600

aagcugaagg aggugcugcu cgacagcacg cagucccgca gcgaccggca gaaggccgcc      660

gccaagcugc ugcuccccac caacgaccug gacaaggaca ccaccaagau ccugaagacg      720

guguccaccg agaucaccaa ggccaucacc gggcucaagg cgaaguucuu cgucaucacg      780

ggcaacgaca ucgccaagga ccagcagaag gacuggaccu ucagcaucga cacccuggac      840

gagaagaugu ccgugaucga gcccagcuuc uccgagaaga acuucagccu gaucaccauc      900

cuccagaagc uguacuccag cgugacgcug gccgggaucg ucccgaacgg ccucagccug      960

uccgaggcca ugauccagaa guacgaccgc cacgccagcg accuggugcu ccugaagaag     1020

uacugggaca ccaagcccaa ggugcugaag caccagcucc agaacaccua cgcggaguac     1080

aucgagaucc accaggucgu ggacgaguuc uacuccgacc ugagcaagcu gaucaagaac     1140

gacgacgaca cccucgccca ggagaucaag uccaagauca cgaucggcaa cuuccugccc     1200

aagcagcgga ccaccgagaa cggggugauc cccuaccagc ugcagcagcg cgagcucgac     1260

accaucauca agaaccagca ggaguacuac ccguggcugg ccgaggagaa ccccgucgcc     1320

gaccaccaga ucgccuuccc cuacaagcug gacgagcucg ugggcuuccg cauccccuac     1380

uacguggggc cgaugaucgc gaaggaggac acgagcaacu ccaagaacag caaguucgcc     1440

uggaugaucc ggaagcaguc cggcgagguc acccccugga acuucgacga gaagaucgac     1500

cgcaccgcca gcgccaccgc cuucaucgac cggaugacga ccaccgacac cuaccugauc     1560

ggggaggacg ugcugcccaa gcagucccuc aucuaccagc gcuucgaggu gcugaacgag     1620

cugaacaaga ucagcaucga cggccagccc aucacgcgga agcagaagca guccgucuac     1680

acccacgugu ucgagaagaa gaagaccguc caggugaagg acgugcagaa guuccugguc     1740

agcuccggcg aggugaugag ccucccgaag aucgggggcc uggcgaacga gaagcaguuc     1800

aacuccagcc uguccacccu cuacgacuac aacaagauca uccccgacgc ccuggaggac     1860

cgcaacaagg uggacgacau cgagaagauc auccugugga gcacgaucuu cgaggacaac     1920

agcaucuucg ucgagaagcu cgacuccauc gacuggcuga ccagcuccca gcggaagcgc     1980

cuggcccaga agcgguaccg cggguggggc cggcucagcc ugcgccugcu caccaccuuc     2040

aagaacaagg acggguacuc cauccuggac gagcugugga acagcaacca caacuucaug     2100

gagauccagg cccaguccga cuucagcgag cagaucgcga ccgccaacaa caacaaccuc     2160

ucccaggugc ugcccgccaa gugggagccc ggccggaaga accuggccag ccucgacgag     2220

uucaucucca accuguacac gagcccgcag aacaagaagg cccugcgcaa ggcgauccgg     2280

gugguccagg acaugaugac cgccgccggc cacaugcccg agaagaucuu caucgaggug     2340

gcccgcgaca ccuccaacag ccaccagcag accaucgccc ggcagcgcca gcuccagcag     2400

cuguaccagu ccgaggacaa ggaggugauc gacagcuccg uccggagcga gcuggacgac     2460

auccucaaga accgcgcgaa caucaaggac cagcuguucc uguacuucca gcagggcggg     2520

cgcgaccucu acacggggaa ggccaucaac aucgacaagc ugagccugua ccacaucgac     2580

cacauccucc cccagucccu ggugaaggac gacagccugg acaaccgggu gcucgucucc     2640

cagaagacca acgaggacaa gggcgugacc uucgugagcg acaaguacau gaacaacaug     2700

cugcccaccu ggaagcgcug gcacgagaug gggcugaucu ccgcccggaa gcucaagaac     2760

cugacgaugc gcccggacga gaucgacaag uacgcccccg gcuucaucca gcggcagcug     2820

gucgagaccc gccaggugac caagcucgug aucgagcugc uggacgcccu ccugggcaac     2880

agccagaccg agaucaucac gaucaaggcg ucccugagcc accaguuccg gcagcaguuc     2940

gaccucguca agauccgcga ggugaacgac aaccaccacg ccuucgacgc cuuccuggcc     3000

gcgcggaucg ggaccuaccu guacaagcgc uaccccaagc uccggccgua cuucguguac     3060

ggccaguaca ugcagaacca caagaaccug aaguccauca accugaucaa gacccucgac     3120

agcgacaagg ccaucgucga cgaggggacc ggcgagaucc ugugggacaa gaaguccgac     3180

cuggccaaga ugaucaagau cuacaacuac cagcgccucc ugaucacgca cgagagccgg     3240

accuccuucg gcgccaugua caagcagaag auccugaagg ccaccgacgc gaagcagaac     3300

gggggcgggu ucaagcucgu gcccaccaag cgcaaccggc ccguggacau cuacggcggg     3360

uacacgggcc agaccgccgc cuaccugacc aucguccgcg uguacagcaa gaagaccucc     3420

gagcugcggg ugcuccccau cuacacgaac aagguccagc agaucgagaa ggccaugacc     3480

cagggcgaca aggccaccau gcuggcguuc aagaagguga ucgccccgca gcuggggaag     3540

gagaacaaga agacccacga gaucaagauc cccgaguucg acguggucau ccccaaggug     3600

cuccuggacc agcuggugau cgacgacauc gccggcagcc cccaccgcuu ccggcucggg     3660

acggacaccu acuaccacaa cucccagcag cuguuccugc cgcucagcau gcagcgccag     3720

cugauggacc gggaguccga cgacaagguc uaccuggaca ucuucaacga cgugcucaag     3780

caggucgaca gcuacuucaa ccuguacgac gugaacggcu uccgcgccgc ccugcagaag     3840

uccaagaagg cguucgagca gcuccccauc aacgacuucc ccaaggacgu gcccgacaac     3900

aagaagaaga aggggaagcg cagcauccug aaggagaucu ucaucggccu gcacgccaac     3960

gccacccucg gcaaccugaa gcagaugggg aucaagaccg acuucggcaa gcugcagaag     4020

uccaacgggc uccggaucac ggagaacugc aagaucaucc accagagccc gaccggccug     4080

uucgagcgca ucgugcccau caaguga                                         4107


<210>  1955
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1955
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaucgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacaccc gccggaagaa ccgcaucugc      240

uaccugcagg agaucuucuc caacgagaug gccaaggucg acgacagcuu cuuccaccgg      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcgccaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca cgaucuacca ccuccggaag      420

aagcucgugg acagcaccga caaggcggac cugcgccuga ucuaccucgc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

guggacaagc uguucaucca gcugguccag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gcggcgugga cgcgaaggcc auccuguccg cccgccugag caagucccgg      660

cgccucgaga accugaucgc ccagcugccg ggggagaaga agaacggccu cuucgggaac      720

cugaucgccc ugagccucgg ccugaccccc aacuucaagu ccaacuucga ccuggcggag      780

gacgccaagc uccagcugag caaggacacg uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg gcugaacucc gagaucacca aggcgccccu cagcgccagc      960

augaucaagc gcuacgacga gcaccaccag gaccugaccc ugcucaaggc ccuggugcgg     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc aguccaagaa cggguacgcc     1080

ggcuacaucg acgggggcgc cagccaggag gaguucuaca aguucaucaa gccgauccuc     1140

gagaagaugg acggcaccga ggagcugcug gccaagcuga accgcgagga ccuccugcgg     1200

aagcagcgca cguucgacaa cggguccauc ccccaccaga uccaccuggg cgagcuccac     1260

gccauccugc gccggcagga ggacuucuac cccuuccuga aggacaaccg cgagaagauc     1320

gagaagaucc ucaccuuccg gauccccuac uacgucgggc cgcuggcccg cggcaacagc     1380

cgguucgcgu ggaugacccg caaguccgag gagaccauca cgcccuggaa cuucgaggag     1440

gugguggaca agggggccag cgcccagucc uucaucgagc ggaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu ccuccccaag cacagccugc uguacgagua cuucaccgug     1560

uacaacgagc ucaccaaggu gaaguacguc acggagggca ugcgcaagcc ggccuuccug     1620

uccggcgagc agaagaaggc gaucguggac cuccucuuca agaccaaccg gaaggugacc     1680

gucaagcagc ugaaggagga cuacuucaag aagaucgagu gcuucgacag cguggagauc     1740

uccggggugg aggaccgcuu caacgccagc cugggcaccu accacgaccu ccugaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccucga ggacaucguc     1860

cugacgcuga cccucuucga ggaccgggag augaucgagg agcgccugaa gaccuacgcc     1920

caccuguucg acgacaaggu gaugaagcag cucaagcggc gccgguacac cgucuggggg     1980

cgccuguccc ggaagcugau caacggcauc cgcgacaagc agagcgggaa gacgauccuc     2040

gacuuccuga aguccgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

agccucaccu ucaaggagga cauccagaag gcccaggugu ccggccaggg ggacagccug     2160

cacgagcaca ucgcgaaccu ggccggcucc cccgccauca agaaggggau ccuccagacc     2220

gugaaggucg uggacgagcu ggugaagguc augggccgcc acaagcccga gaacaucgug     2280

aucgagaugg cccgcgagaa ccagaccacg cagaaggggc agaagaacag ccgggagcgc     2340

augaagcgga ucgaggaggg caucaaggag cugggcagcc agauccucaa ggagcacccc     2400

guggagaaca cccagcugca gaacgagaag cuguaccucu acuaccugca gaacgggcgc     2460

gacauguacg ucgaccagga gcuggacauc aaccggcucu ccgacuacga cguggaccac     2520

aucgugccgc agagcuucau caaggacgac uccaucgaca acaagguccu gacccgcagc     2580

gacaagaacc ggggcaaguc cgacaacgug cccagcgagg agguggucaa gaagaugaag     2640

aacuacuggc gccagcugcu caacgccaag cugaucaccc agcggaaguu cgacaaccug     2700

acgaaggcgg agcgcggggg ccucuccgag cuggacaagg ccggcuucau caagcggcag     2760

cugguggaga cccgccagau caccaagcac guggcccaga uccucgacag ccggaugaac     2820

accaaguacg acgagaacga caagcugauc cgcgagguca aggugaucac ccugaagucc     2880

aagcucguga gcgacuuccg gaaggacuuc caguucuaca agguccgcga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gcgguggugg ggacggcccu gaucaagaag     3000

uaccccaagc ucgaguccga guucgucuac ggcgacuaca agguguacga cgugcggaag     3060

augcuggcca agagcgagca ggagaucggg aaggccaccg cgaaguacuu cuucuacucc     3120

aacaucauga acuucuucaa gaccgagauc acccuggcca acggcgagau ccgcaagcgg     3180

ccccucaucg agacgaacgg ggagaccggc gagaucgucu gggacaaggg ccgcgacuuc     3240

gccaccgugc ggaaggugcu gagcaugccg caggucaaca ucgugaagaa gaccgaggug     3300

cagaccgggg gcuucuccaa ggagagcauc cugcccaagc gcaacuccga caagcucauc     3360

gcccgcaaga aggacuggga ccccaagaag uacgggggcu ucgacagccc cacggucgcc     3420

uacagcgugc ugguggucgc gaagguggag aaggggaagu ccaagaagcu caagagcgug     3480

aaggagcugc ugggcaucac caucauggag cgguccagcu ucgagaagaa cccgaucgac     3540

uuccuggagg ccaagggcua caaggagguc cgcaaggacc ucaucaucaa gcugcccaag     3600

uacucccugu ucgagcucga gaacgggcgg aagcgcaugc uggccagcgc cggcgagcug     3660

cagaagggga acgagcucgc ccugcccucc aaguacguga acuuccugua ccucgcgagc     3720

cacuacgaga agcugaaggg cuccccggag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccucga cgagaucauc gagcagauca gcgaguucuc caagcggguc     3840

auccuggccg acgccaaccu ggacaaggug cucagcgccu acaacaagca ccgcgacaag     3900

cccauccggg agcaggccga gaacaucauc caccuguuca cccugacgaa ccucggggcg     3960

cccaccgccu ucaaguacuu cgacaccacc aucgaccgca agcgguacac guccaccaag     4020

gaggugcugg acgccaccuu cauccaccag agcaucaccg gccuguacga gacgcgcauc     4080

gaccucuccc agcugggcgg ggacuga                                         4107


<210>  1956
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1956
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcaucugc      240

uaccugcagg agaucuucuc caacgagaug gccaaggucg acgacagcuu cuuccaccgg      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcgccaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccggaag      420

aagcucgugg acagcaccga caaggcggac cugcgccuga ucuaccucgc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

guggacaagc uguucaucca gcugguccag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gcggcgugga cgcgaaggcc auccuguccg cccgccugag caagucccgg      660

cgccucgaga accugaucgc ccagcugccg ggggagaaga agaacggccu cuucgggaac      720

cugaucgccc ugagccucgg ccugacgccc aacuucaagu ccaacuucga ccuggcggag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg gcugaacucc gagaucacca aggccccgcu cagcgccagc      960

augaucaagc gcuacgacga gcaccaccag gaccugaccc ugcucaaggc ccuggugcgg     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc aguccaagaa cggguacgcg     1080

ggcuacaucg acgggggcgc cagccaggag gaguucuaca aguucaucaa gccgauccuc     1140

gagaagaugg acggcacgga ggagcugcug gccaagcuga accgcgagga ccuccugcgg     1200

aagcagcgca ccuucgacaa cggguccauc ccccaccaga uccaccuggg cgagcuccac     1260

gccauccugc gccggcagga ggacuucuac cccuuccuga aggacaaccg cgagaagauc     1320

gagaagaucc ucaccuuccg gauccccuac uacgucgggc cgcuggcccg cggcaacagc     1380

cgguucgcgu ggaugacccg caaguccgag gagacgauca cccccuggaa cuucgaggag     1440

gugguggaca agggggccag cgcccagucc uucaucgagc ggaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu ccuccccaag cacagccugc uguacgagua cuucaccgug     1560

uacaacgagc ucacgaaggu gaaguacguc accgagggca ugcgcaagcc ggccuuccug     1620

uccggcgagc agaagaaggc gaucguggac cuccucuuca agaccaaccg gaaggugacc     1680

gucaagcagc ugaaggagga cuacuucaag aagaucgagu gcuucgacag cguggagauc     1740

uccggggugg aggaccgcuu caacgccagc cugggcacgu accacgaccu ccugaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccucga ggacaucguc     1860

cugacccuga cccucuucga ggaccgggag augaucgagg agcgccugaa gaccuacgcc     1920

caccuguucg acgacaaggu gaugaagcag cucaagcggc gccgguacac gggguggggc     1980

cgccuguccc ggaagcugau caacgggauc cgcgacaagc agagcggcaa gaccauccuc     2040

gacuuccuga aguccgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

agccucaccu ucaaggagga cauccagaag gcccaggucu ccgggcaggg cgacagccug     2160

cacgagcaca ucgcgaaccu ggccgggucc cccgccauca agaagggcau ccuccagacc     2220

gugaaggugg ucgacgagcu ggugaaggug auggggcgcc acaagcccga gaacaucguc     2280

aucgagaugg cccgcgagaa ccagacgacc cagaagggcc agaagaacag ccgggagcgc     2340

augaagcgga ucgaggaggg caucaaggag cuggggagcc agauccucaa ggagcacccc     2400

guggagaaca cccagcugca gaacgagaag cuguaccucu acuaccugca gaacggccgc     2460

gacauguacg uggaccagga gcuggacauc aaccggcucu ccgacuacga cgucgaccac     2520

aucgugccgc agagcuucau caaggacgac uccaucgaca acaaggugcu gacccgcagc     2580

gacaagaacc gggggaaguc caacaacguc cccagcgagg agguggugaa gaagaugaag     2640

aacuacuggc gccagcugcu caacgccaag cugaucacgc agcggaaguu cgacaaccug     2700

accaaggcgg agcgcggcgg ccucuccgag cuggacaagg ccggguucau caagcggcag     2760

cuggucgaga cccgccagau caccaagcac guggcccaga uccucgacag ccggaugaac     2820

accaaguacg acgagaacga caagcugauc cgcgagguga aggucaucac gcugaagucc     2880

aagcucguga gcgacuuccg gaaggacuuc caguucuaca aggugcgcga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gcggucgugg gcaccgcccu gaucaagaag     3000

uaccccaagc ucgaguccga guucguguac ggggacuaca aggucuacga cgugcggaag     3060

augaucgcca agagcgagca ggagaucggc aaggccaccg cgaaguacuu cuucuacucc     3120

aacaucauga acuucuucaa gaccgagauc acgcuggcca acggggagau ccgcaagcgg     3180

ccccugaucg agaccaacgg cgagaccggc gagaucgugu gggacaaggg gcgcgacuuc     3240

gccaccgucc ggaaggugcu cagcaugccc caggugaaca ucgucaagaa gacggaggug     3300

cagaccggcg gguucuccaa ggagagcauc cugcccaagc gcaacuccga caagcugauc     3360

gcccgcaaga aggacuggga ccccaagaag uacggcgggu ucgacagccc caccguggcc     3420

uacagcgucc ucgugguggc gaaggucgag aagggcaagu ccaagaagcu caagagcgug     3480

aaggagcugc ugggcaucac caucauggag cgguccagcu ucgagaagaa cccgaucgac     3540

uuccuggagg ccaaggggua caaggaggug cgcaaggacc ugaucgucaa gcuccccaag     3600

uacucccugu ucgagcugga gaacggccgg aagcgcaugc ucgccagcgc cggggagcug     3660

cagaagggca acgagcuggc ccuccccucc aaguacguga acuuccugua ccuggcgagc     3720

cacuacgaga agcucaaggg guccccggag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccugga cgagaucauc gagcagauca gcgaguucuc caagcggguc     3840

auccucgccg acgccaaccu ggacaaggug cugagcgccu acaacaagca ccgcgacaag     3900

cccauccggg agcaggccga gaacaucauc caccucuuca cgcugaccaa ccugggcgcg     3960

cccgccgccu ucaaguacuu cgacaccacc aucgaccgca agcgguacac guccaccaag     4020

gaggugcucg acgccaccuu cauccaccag agcaucaccg gccuguacga gacgcgcauc     4080

gaccuguccc agcucggggg cgacuga                                         4107


<210>  1957
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1957
augagccgcc ccuacaacau cggccuggac aucgggaccu ccagcaucgg cugguccgug       60

gucgacgacc agagcaagcu cguguccgug cgggggaagu acggcuacgg gguccgccug      120

uacgacgagg gccagaccgc cgccgagcgg cgcagcuucc ggacgacccg ccggcgccug      180

aagcggcgca aguggcggcu cggccugcug cgcgagaucu ucgagccgua caucaccccc      240

aucgacgaca ccuucuuccu ccggcagaag caguccaacc ugagccccaa ggaccagcgc      300

aagcuguaca cgcagaccuc ccucuucaac gaccggaccg accgcgcguu cuacgacgac      360

uaccccacca ucuaccaccu ccgguaccgc cugaugacgg agaagcggca guucgacauc      420

cgcgagaucu accuggccau gcaccacauc gugaaguacc gggggcacuu ccugaacgag      480

gccccgguga gcuccuucaa gagcuccgag aucaaccugg ucgcccacuu cgaccgccuc      540

aacaccaucu ucgccgaccu guucagcgag uccggcuucc agcuggagac cgacaagcuc      600

gcggagguga aggcccugcu gcucgacaac cagcagagcg ccuccaaccg ccagcggcag      660

gcccugagcc ugaucuacac ccccuccacg aacaaggcgg uggagaagca gaacaaggcc      720

aucgccaccg agcuccugaa ggccauccug gggcucaagg ccaaguucaa cguccugacc      780

ggcaucgagg cggaggacgu gaaggccugg acccugacgu ucaacgccga gaacuucgac      840

gaggagaugg ugaagcucga gagcagccug gacgacaacg cccaccagau caucgagucc      900

cugcaggagc ucuacagcgg gguccugcug gccggcaucg ugcccgagaa ccagucccuc      960

agccaggcga ugaucaccaa guacgacgac caccagaagc accugaagau gcugaaggcc     1020

gugcgcgagg cccucgcccc cggcgaccgg cagcgccuga agcaggccua cgaccaguac     1080

gucgacgggc aggagaacac caaggcguac uccaaggagg acuucuacgg cgacaucacc     1140

aaggcccuga agaacaaccc ggaccacccc aucgugagcg agaucaagaa gcucaucgag     1200

cuggaccagu ucaugcccaa gcagcggacg aaggacaacg gggccauccc ccaccagcug     1260

caccagcagg agcucgaccg caucaucgag aaccagcagc aguacuaccc guggcuggcc     1320

gagcugaacc ccaacuccaa gcggcagacc guggccaagu acaagcucga cgagcugguc     1380

gcguuccgcg ugcccuacua cgugggcccc cugaucaccg ccgagcagca gcggcagagc     1440

uccgacgcca aguucgccug gcugauccgc aaggcggagg gccggaucac cccguggaac     1500

uucgacgaca aggucgaccg ccaggccagc gccaacgagu ucaucaagcg gaugacgacc     1560

accgacaccu accuccuggc cgaggacgug cugcccaagc agucccucau cuaccagcgc     1620

uucgaggugc ugaacgagcu gaacgggcuc aagaucgacg accagcccau cacgaccgag     1680

cugaagcagg ccaucuucac cgaccuguuc augcagaaga ucagcgucac cgugaagaac     1740

auccaggacu accucgucuc cgagaagcgg uacgcgagcc gccccgccau cacgggccug     1800

uccgacgaga acaaguucaa cagccggcug uccaccuacc acgaccucaa gaugaucgug     1860

ggggacgccg uggacgacgu cgacaagcag gccgaccugg agaagugcau cgaguggagc     1920

accaucuucg aggacggcaa gaucuacucc gccaagcuga acgagaucga cuggcucacc     1980

gaccagcagc gcgugcagcu ggcggccaag cgguaccgcg gguggggccg ccugagcgcc     2040

aagcuccuga cgcagaucgu gaacgccaac ggccagcgga ucauggaccu gcucugggac     2100

accaccgaca acuucaugcg caucguccac agcgaggacu ucgacaagcu gaucaccgag     2160

gccaaccaga ugaugcuggc ggagaacgac gugcaggacg ugaucaacga ccucuacacc     2220

uccccgcaga acaagaaggc ccugcggcag auccugcucg ucgugaacga cauccagaag     2280

gccaugaagg ggcaggcccc cgagcgcauc cugaucgagu ucgcgcggga ggacgaggug     2340

aacagccgcc uguccgucca gcggaagcgc cagguggagc aggucuacca gaacaucagc     2400

aacgagcucc ugaacaacac ggagauccgg aacgagcuga aggaccucuc caacagcgcc     2460

cuguccaaca cccgccuguu ccucuacuuc augcagggcg ggcgggacau guacaccggc     2520

gacagccuga acaucgaccg ccuguccacc uacgacaucg accacauccu cccccagagc     2580

uucaucaagg acaacucccu ggacaaccgg gugcugguga gccagaagau gaaccgcucc     2640

aaggccgacc aggugcccac ggacuucacc agcgucgagc ucgggcagaa gaugcagauc     2700

cagugggagc agaugcugcg ggccggccug aucaccaaga agaaguacga caaccucacc     2760

cugaacccgg accacaucuc caaguacgcc augaagggcu ucaucaaccg ccagcuggug     2820

gagacgcgcc aggugaucaa gcucgcgacc aaccugcuga uggagcagua cggggaggac     2880

aacaucgagc ucaucaccgu caagagcggc cugacccacc agaugcggac ggaguucgac     2940

uuccccaaga accggaaccu gaacaaccac caccacgccu ucgacgccua ccucaccgcc     3000

uucguggggc uguaccugcu caagcgcuac cccaagcuga agcccuacuu cguguacggc     3060

gaguaccaga aggccuccca gcaggacaag uggcggaacu ucaacuuccu gaacgggcuc     3120

aagaaggacg agcuggucga cgagaacacc gaggcgguga ucugggacaa ggagagcggc     3180

cuggccuacc ucaacaagau cuaccaguuc aagaagaucc uggugacccg cggcguccac     3240

gagaacagcg gggcccuguu caaccagacg cucuacgccg ccaaggacga caaggcgucc     3300

ggccagggcg ggaagcagcu gaucccggcc aagcaggacc gcagcaccgc ccuguacggc     3360

ggguacuccg gcaagaccgu ggccuacaug ugcaucgugc ggaucaagaa caagaagggg     3420

gaccucuaca aggucugcgg cguggagacc agcuggcugg cgcagcugaa gcagcucacg     3480

gacgaggacu cccagaaggc cuuccuggag cagaagauca gcccccaguu caccaaggug     3540

aagaagcaga aggggaacau caucgaggcc gucgaggagu ucgaggugau cgcgccccac     3600

auccugauca accagcgcuu cuucgacaac ggccaggagc ucacccuggg cuccgccacc     3660

uacaagcaca acgagcagga gcugauccuc gacaagacgg ccgugaagcu gcugaacggg     3720

gcccucccgc ugacccagag cgaggagcug gccgagcagg ucuacgacga gauccucgac     3780

caggugaugc acuacuuccc ccuguacgac accaaccagu uccgggccaa gcuguccacc     3840

ggcaaggccg cguucaugaa gcuccccugg aagucccagu gggacgggaa caagauggug     3900

caggucggcc agcaggucau ccuggaccgc auccugaucg ggcuccacgc caacgccgcc     3960

augagcgacc ugaaggugcu gaagaugacg acccccuucg gcaugaucca gcagagcacc     4020

ggcaucaccc ucuccgccga cacgcagauc aucuaccaga gcccgaccgg gcuguucgag     4080

cggcgcgugg cgcugcggga ccuguga                                         4107


<210>  1958
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1958
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcaucugc      240

uaccugcagg agaucuucuc caacgagaug gccaaggucg acgacagcuu cuuccaccgg      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcgccaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccggaag      420

aagcucgugg acagcaccga caaggcggac cugcgccuga ucuaccucgc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

guggacaagc uguucaucca gcugguccag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gcggcgugga cgcgaaggcc auccuguccg cccgccugag caagucccgg      660

cgccucgaga accugaucgc ccagcugccg ggggagaaga agaacggccu cuucgggaac      720

cugaucgccc ugagccucgg ccugacgccc aacuucaagu ccaacuucga ccuggcggag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg gcugaacucc gagaucacca aggccccgcu cagcgccagc      960

augaucaagc gcuacgacga gcaccaccag gaccugaccc ugcucaaggc ccuggugcgg     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc aguccaagaa cggguacgcg     1080

ggcuacaucg acgggggcgc cagccaggag gaguucuaca aguucaucaa gccgauccuc     1140

gagaagaugg acggcacgga ggagcugcug gccaagcuga accgcgagga ccuccugcgg     1200

aagcagcgca ccuucgacaa cggguccauc ccccaccaga uccaccuggg cgagcuccac     1260

gccauccugc gccggcagga ggacuucuac cccuuccuga aggacaaccg cgagaagauc     1320

gagaagaucc ucaccuuccg gauccccuac uacgucgggc cgcuggcccg cggcaacagc     1380

cgguucgcgu ggaugacccg caaguccgag gagacgauca cccccuggaa cuucgaggag     1440

gugguggaca agggggccag cgcccagucc uucaucgagc ggaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu ccuccccaag cacagccugc uguacgagua cuucaccgug     1560

uacaacgagc ucacgaaggu gaaguacguc accgagggca ugcgcaagcc ggccuuccug     1620

uccggcgagc agaagaaggc gaucguggac cugcuguuca agaccaaccg gaaggugacc     1680

gucaagcagc ugaaggagga cuacuucaag aagaucgagu gcuucgacag cguggagauc     1740

uccggggugg aggaccgcuu caacgccagc cugggcacgu accacgaccu ccugaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccucga ggacaucguc     1860

cugacccuga cccucuucga ggaccgggag augaucgagg agcgccugaa gaccuacgcc     1920

caccuguucg acgacaaggu gaugaagcag cucaagcggc gccgguacac gggguggggc     1980

cgccuguccc ggaagcugau caacgggauc cgcgacaagc agagcggcaa gaccauccuc     2040

gacuuccuga aguccgacgg cuucgccaac cggaacuuca ugcagcugau ccacgacgac     2100

agccucaccu ucaaggagga cauccagaag gcccaggucu ccgggcaggg cgacagccug     2160

cacgagcaca ucgcgaaccu ggccgggucc cccgccauca agaagggcau ccuccagacc     2220

gugaaggugg ucgacgagcu ggugaaggug auggggcgcc acaagcccga gaacaucguc     2280

aucgagaugg cccgcgagaa ccagacgacc cagaagggcc agaagaacag ccgggagcgc     2340

augaagcgga ucgaggaggg caucaaggag cuggggagcc agauccucaa ggagcacccc     2400

guggagaaca cccagcugca gaacgagaag cuguaccucu acuaccugca gaacggccgc     2460

gacauguacg uggaccagga gcuggacauc aaccggcucu ccgacuacga cgucgaccac     2520

aucgugccgc agagcuucau caaggacgac uccaucgaca acaaggugcu gacccgcagc     2580

gacaagaacc gggggaaguc cgacaacguc cccagcgagg agguggugaa gaagaugaag     2640

aacuacuggc gccagcugcu caacgccaag cugaucacgc agcggaaguu cgacaaccug     2700

accaaggcgg agcgcggcgg ccucuccgag cuggacaagg ccggguucau caagcggcag     2760

cuggucgaga cccgccagau caccaagcac guggcccaga uccucgacag ccggaugaac     2820

accaaguacg acgagaacga caagcugauc cgcgagguga aggucaucac gcugaagucc     2880

aagcucguga gcgacuuccg gaaggacuuc caguucuaca aggugcgcga gaucaacaac     2940

uaccaccacg cccacgacgc cuaccugaac gcggucgugg gcaccgcccu gaucaagaag     3000

uaccccaagc ucgaguccga guucguguac ggggacuaca aggucuacga cgugcggaag     3060

augaucgcca agagcgagca ggagaucggc aaggccaccg cgaaguacuu cuucuacucc     3120

aacaucauga acuucuucaa gaccgagauc acgcuggcca acggggagau ccgcaagcgg     3180

ccccugaucg agaccaacgg cgagaccggc gagaucgugu gggacaaggg gcgcgacuuc     3240

gccaccgucc ggaaggugcu cagcaugccc caggugaaca ucgucaagaa gacggaggug     3300

cagaccggcg gguucuccaa ggagagcauc cugcccaagc gcaacuccga caagcugauc     3360

gcccgcaaga aggacuggga ccccaagaag uacggcgggu ucgacagccc caccguggcc     3420

uacagcgucc ucgugguggc gaaggucgag aagggcaagu ccaagaagcu caagagcgug     3480

aaggagcugc ucggccugac caucauggag cgguccagcu ucgagaagaa cccgaucgac     3540

uuccuggagg ccaaggggua caaggaggug aagaaggacc ucaucaucaa gcugcccaag     3600

uacucccugu ucgagcucga gaacggccgc aagcggaugc uggccagcgc cggggagcug     3660

cagaagggca acgagcucgc ccugcccucc aaguacguca acuuccugua ccucgcgagc     3720

cacuacgaga agcugaaggg guccccggag gacaacgagc agaagcagcu guucguggag     3780

cagcacaagc acuaccucga cgagaucauc gagcagauca gcgaguucuc caagcgcgug     3840

auccuggccg acgccaaccu ggacaagguc cucagcgccu acaacaagca ccgggacaag     3900

cccauccgcg agcaggccga gaacaucauc caccuguuca cgcugaccaa ccucggcgcg     3960

cccgccgccu ucaaguacuu cgacaccacc aucgaccgga agcgcuacac guccaccaag     4020

gaggugcugg acgccacccu gauccaccag agcaucaccg gccucuacga gacgcggauc     4080

gaccuguccc agcucggggg cgacuga                                         4107


<210>  1959
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1959
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga ccgucaacga gaaguccagc aucgugaagc ucggggugcg ggucaacccc      120

cugacggugg acgagcagaa gaacuucgag gcgggcaagu ccaucccgac caacgccggg      180

cgcacccuga agcggggcau gcgccggaac cuccagcgcu acaagcugcg gcgcgacuac      240

cugauccagu gccucaagga guacggcugg aucaccagcg acacgcugcu guacgagugc      300

gggaaccgga ccaccuucga gaccuaccgc cuccgggcca aggccgugga cgaggagauc      360

gcccuggagg agcugggccg cguccuccug augaucaaca agaagcgggg guacaagucc      420

aaccgcaagg cgaaguccuc cgacgagggc cagcugaucg acgggauggc caucgccaug      480

gacauguacc acaacggcac gacccccggc gaguacgugu acagccuccu guccaagggg      540

aagaaguaca uccccguguu cuaccggagc gaccugcucg acgaguucga ccgcaucugg      600

gacuaccagu gcgccuacua ccccgacauc cugaccuccc aguucaagga gcugauccgg      660

ggcaagagcu cccaggucac cagcaagcug uucaagcagc aguacaaccu guucacggcg      720

gacaacaagg agaaggacaa gcgccugcag gccuacugcc ggcgcgccga cgcccucaag      780

aagcggcugc cgguggagga gguggccgcg gcccugugcg acgucaaccg cgacgugaac      840

gccuccagcg gguaccucgg caccaucucc gaccgcagca agaagcuguu cuucaacaag      900

cagaccgugg gccaguaccu gaugcucgag cuggacaagg acucccacuu cagcgucaag      960

aacgaggugu ucuaccggca ggacuaccug aacgaguucg aggccaucug ggagaagcag     1020

gccgaguacc accccgagcu cacccccgac cgcaagaaga uccugcggga cguggucauc     1080

uucuaccagc gcccccucaa gagccagaag gaccucgugg cgugcugcga gcuggagcgg     1140

cgccggaucg aggugaugaa ggacgggaag guccgcuccg uggagaucgg cccgaaggug     1200

ugccccaaga gcagcccccu guuccaggac uucaagaucu ggcacguccu caacaaccug     1260

aaggugcggg acaaggccac gggggacgag cgcuuccugg agcucgacga gaaggagauc     1320

cuguucagcg agcuggccau caaguccaag cucagcaaga ccgaggcccu gaagcugcug     1380

uaccagaagc ccaagggccu cgaccugaac uacgacuccg ucgaggggaa caccacccag     1440

gccgcgcugu ucgaggccua ccagacggug aucgccauga ccggccacgg cgaguacgug     1500

uucagcaaga uguccgccag cgaggucaug uccguggugg agggggucuu cggcgggcuc     1560

ggcuucaaca ccagcgugcu gcgguucgac ucccugcucc aggggaagga gcuggagaag     1620

caggacagcu accgccugug gcaccuccug uacuccuacg agggcgacaa cagcgugacc     1680

ggcacggacg ggcuggucgc caagguggcg ucccuccugc ggugcgacga ggaguacgcc     1740

aagguggucg ccggcgugac cuuccagccg gacuacggga gccuguccgc caaggcgauc     1800

cgccggaucc ucccccacau gcgcgccggc aacgaguaca gccuggccug cgaguacgcc     1860

ggguacaagc acagccggcg cucccugacc aaggaggagc ucgagcagaa ggacuacaag     1920

gaccaccugg agcugcuccc ccggaacagc cugcgcaacc ccguggucga gaagauccug     1980

aaccagaugg ugaacguggu caacgccgug guggacgcgu acggcaagcc ggacgagauc     2040

cgcaucgaga uggcccggga gcucaagaag uccgccaagg agcgcgagga gaugaccgcc     2100

gccaucaaca aggcgacgag cgagcacgag cggaucaagg agauccugaa caaggaguuc     2160

ggcauccagc acgucuccca caacgacauc gugcgcuaca agcuguacug cgagcucaag     2220

aacaacgggu acaagacccu guacagcgac accuacaucc cccaggagaa gcuguucucc     2280

aaggaggugg acaucgagca cauccucccc caggcccggc uguucgacga cagcuucucc     2340

aacaagaccc uggaguaccg cagcgucaac aucgagaagu ccaaccagac ggccugggac     2400

uacguggccg ccaagugggg cgaggagggg agccuccggu acaagaagcg cguggacgac     2460

cugcuggcgg ccggcgucau cggcaagucc aagcacgaca agcuccugau gaagcaggag     2520

gacaucccca ccgacuucau caaccgggac cugcgcgaca cccaguacau cgcccggaag     2580

gcccgcgaga uccucgagga ccuggugccg auggugguca gcaccacggg ggcggugacc     2640

gaccggcugc gcgaggacug gcagcucgug gacgucaugc aggagcugaa cugggacaag     2700

uacgccauga agggccugac cgaggagugg accgacaagg acgggcggcg caucucccgg     2760

aucaaggagu ggaccaagcg caacgaccac cggcaccacg ccauggacgc ccucacgauc     2820

gccuucacca agcacagcuu cauccaguac cugaacaacc ugaacgcgcg cuccgacaag     2880

agcggcagca ucuacgccau cgagcagaag gagcucuacc gggacaacca cgggaagcug     2940

cgcuucauca ugcccuucga gggcuuccgc uccgaggcca agcggcagcu ggagaacauc     3000

cucgugagca ucaaggccaa gaacaaggug gucacccgca acgugaacaa cgcccggcgc     3060

ggcuccgggc ggcaggcgaa ggugcagcug acgccccgcg gccagcugca caacgagacc     3120

aucuacggga gcauccaccg guacgucacc aagcucgaga aggugggcgg gaacuucgac     3180

ggcgagcaga ucgccaaggu ggccaaccug cgcuaccggg aggcccugca ggcccgccac     3240

gaggcguucg gcggggacgc caagaaggcc uucaccggca agaacgcccu cgacaagaac     3300

ccccuguacc ugaaggacac ccggacggag gucccgcuca aggugaagac cguguccacc     3360

gagacccugu acacgauccg caaggcgguc gcccccgacc ugaacgugga caaggugauc     3420

gacgccaagg uccggaaggu gcucgaggag cgccugcagg aguacggggg cgaccccaag     3480

aaggccuucg ugaaccugga cgagaacccc aucuaccuca acaaggccaa ggggaucacc     3540

aucaagcggg ucgcgaucac cggcgugaac aacgccaucg cccugcacga caagaaggac     3600

gucggcgggc acgugaugcu ggacgccagc ggccgcaagc agccggugga cuucgucaac     3660

accgggaaca accaccacgu ggccaucuac cgggacgcgg agggcucccu ccaggaggug     3720

gucgugagcu ucuacgaggc cguggcccgc gucucccugg ggcugcccgu gaucgaccgg     3780

gaguaccgca aggaggacgg cuggcaguuc cucuucacga ugaagcagaa cgaguacuuc     3840

guguucccca acccggagac cggcuucaac ccccaggagg ucgaccugac cgacgagacc     3900

aacuacgccc ugaucagccc caaccucuuc cgcgugcaga aguucacgau caaggacuac     3960

uucuuccggc accaccugga gaccaccgug gaggaccgca aggagcugaa ggacaugacc     4020

uggaagcggu gcgggcucgg cgggaucauc ggcaucguca aggugcgcgu gaaccacauc     4080

ggccggaucg ucuccguggg ggaguacuga                                      4110


<210>  1960
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1960
auggccaaga ccuacaacau cggccuggac aucgggacca acagcaucgg cugggccgug       60

gucgacgacc agaaccaccu cauguccauc aaggggaagu acggcuacgg ggugcgccug      120

uacgacgagg gcaagacggc ggaggagcgg cgcagcuucc ggaccacccg ccggcgccug      180

aagcggcgca aguggcggcu cucccugcug cgcgagaucu ucgagcccca gaucaccccg      240

cucgacaaca cguucuuccu gcggcagaag cagagcuccc ugagcuccaa ggacaaccgc      300

aagcucuacc ccgagaugag ccuguucaac gaccggaccg acaccgacuu cuacacccag      360

uaccccacga ucuaccaccu gucccgcgac cucaugacca agaacaagca guucgacauc      420

cgggagaucu accuggccau guaccacauc gugaaguacc gcggccacuu ccugaacgag      480

agccccgucu ccagcuucaa guccagcgag aucaagcucg ccgagaacuu cgagacccug      540

aaccagaucu ucaacgagcg guucaacgag uccauguucc agcuggccgu gaacgacuuc      600

gacgaccuca aggcccugcu gcucaacgac cagcagagcg cguccaaccg ccagaagaag      660

gcccugggga ugaucuacgu gccgaacgag aacaagagcc uggagaagca gaacaaggcc      720

aucgccaccg agcuccugaa ggcgauccug ggccucaagg ccaaguucaa cguccugacg      780

gggaucgagg uggacgacgc caagcccugg acccuguccu ucaacagcga gagcuucgac      840

gaggacaucg acaaggccgc caccaacauc gacgacgacu cccgggagau caucgcgauc      900

cuccagggcc uguacagcgg gauccugcuc gccggcauca uccccgagaa ccagucccug      960

agcgaggcca ugguggagaa guacgaccag cacgccgccc accugaagca gcucaagucc     1020

aucuaccccc ugcugagcau cuccgacaag aaggcgaucc agcaggccua caacuacuac     1080

aucgacggcc aggacaacgc caaggccuac agccgcgagg acuucuacgc cgcgaccacg     1140

aaggcccuca aguccuacca ggacaacgag accaucgccg agauccugaa gcugaucgag     1200

cucgagcagu ucaugccgaa gcagcggacc aaggacaacg gggccauccc ccaccagcug     1260

caccagcagg agcuggagcg caucaucgag aaccagcaga aguacuaccc cuggcucgcc     1320

gagcugaacc ccaacgagag ccgccggaag aucgcgaagu acaagcugga cgagcucguc     1380

gccuuccgcg ugccguacua cgugggcccc cugaucaccu ccgagcagca gcagcagagc     1440

uccaacaaga aguucgccug gaugauccgg aaggccgagg ggaagaucac gcccuggaac     1500

uucgacgaga aggucgaccg ccaggcgagc gccaacgagu ucaucaagcg gaugaccacc     1560

accgacacgu accugcuggc cgaggacgug cuccccaagc agucccuggu guaccagcgc     1620

uucgaggucc ugaacgagcu caacggcauc aagaucgacg acgugccgau caccguggag     1680

cagaagcagc ggaucuucaa cgaccuguuc augaugcagg ccagcgucuc cgugaagaac     1740

auccaggacu accuggugag caagggcuac uucgccuccc gccccgagau caccgggcuc     1800

agcgacgaga acaaggucau cagccggcug uccaccuacc acgaccugaa ggcgauccuc     1860

ggcgggaucg uggacgaggc caacaagcag gccgaccugg agaagugcgu ggaguggagc     1920

acgaucuucg aggacggcaa gaucuucguc gagaagcuga acgagaucga guggcucacc     1980

gacgagcagc gcaagcggcu guccagcaag cgcuaccggg gguggggccg ccuguccaac     2040

aagcuccuga accagaucgu gaacgagaac ggccagcgga ucauggaccu gcucugggac     2100

accaccgaca acuucaugcg caucguccac agcgaggagu ucgacaagcu gaucuccgcc     2160

gccaaccaga cgcugcucag caagaacgac gugcaggacg ugaucaacga ccuguacacc     2220

uccccgcaga acaagaaggc gcugcggcag auccuccugg ucgugagcga cauccagaag     2280

gccaugaaga accaggcccc cuccaagauc cugaucgagu ucgcccgcga ggacgaggcc     2340

aacccgcggc ucagcgugca gcgcaagcgc caggucgagc aggucuacca gaacaucucc     2400

aaggacaucc ugagcgacuc cgacgugaag gcggagcuga aggaccucac caacaccgcc     2460

cuggccaaca cgcggcuguu ccucuacuuc augcagggcg ggcacgacau guacaccggg     2520

gagacccuga acaucgaccg ccugagcgag uacgacaucg accacauccu cccccaggcc     2580

uucaucaagg acaacucccu ggacaaccgg gugcugguga gcgcgaagau gaaccgcagc     2640

aaguccgacc aggugccccu caacgacuac accgaggagc gguucggcag caagaugcgc     2700

guccaguggg agcagaugcu gcgggccggg cugaucacca agcgcaagua cgacaaccuc     2760

acgcugaacc ccgaccggau cuccaaguac gccaugaagg gcuucaucaa ccgccagcug     2820

guggagaccc gccaggugau caagcucguc accaaccugc ugauggacca cuacgaggac     2880

gacgaggugc agcucaucac cgucaagagc ggccugaucc accagaugcg ggagauguuc     2940

gacuucccga agaaccggaa ccugaacaac caccaccacg ccuucgacgc cuaccucacg     3000

gcguucgugg ggaucuaccu gcugaagcgc uaccccaagc ucacccccuu cuucguguac     3060

ggcuccuacc agaagaacaa caccaacgac aaguggcgga acuucaacuu ccugaacgac     3120

cugaagaagg aggaggugau cgacagcgac ucccacgagg ucaucuggaa caaggcccag     3180

gggcucgugg agcuggacaa gaucuaccag uucaagaaga uccuggugac ccgcgagguc     3240

cacgagaaca cgggcgcccu cuacaaccag acccuguaca gcgccaagga cgacaaggcc     3300

uccgggcagg gcggcaagca gcugaucccc gcgaagcagg accggccgac cgcccucuac     3360

gggggcuaca gcgggaagac cguggccuac augugcaucg ugcgcaucaa guccaagaag     3420

gaggacacgu acaaggucug cggcgugaag accagcuggg uggccgagcu ggagcggcug     3480

uccgacagca acgagcagcu cgccucccug aaggcgaagc ugaugcccaa guucaccaag     3540

aacaagaagc agaagggcaa gaccauccag gucacggagg acuucgagau caucgugccc     3600

cgcgugcuca ucaaccagcg guucuacgac aacgggcagg agaucacccu gggcagcgcc     3660

accuacaagc acaacgagca ggagcugaug cucaacaaga aggccgucaa gcugcugaac     3720

gggcagcucu cccuggugga ggccgagacc cuggcgcccc agguguacga cgagauccuc     3780

aagcagguca ugaaguacuu cccccuguac gacacgaacc aguuccgcac caagcugaag     3840

augggcaagg agcaguucau caagcucccc uggaagaaca agugggacgg gaacaagcug     3900

auccacgugg gccagcagac cgugcuggac cgcauccuca ucggccugca cgccaacgcc     3960

gccgucagcg accuguccac ccucaaggug acgacccccu ucgggaagcu gcagcagccg     4020

agcggcaucg cccugagccu caacaccaag aucaucuacc agucccccac cgggcuguuc     4080

gagcggcgcg uggcgcugcg gggccucuga                                      4110


<210>  1961
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1961
auggacaagc ccuacaacau cggccuggac aucgggagcc acuccaucgg cuggaccgug       60

gucgacgaca acagcaagcu cgugcgcauc aaggggaagc ugggcuacgg ggugcggcug      120

uucgacgagg gcaagaccgc cgaggagcgc cggcaguucc gcacgacccg gcgccggcuc      180

aagcgccgga aguggcgccu gcggcugcuc cgcgagaucu ucgagccgca caucgcgccc      240

cuggacgaga ccuucuuccu gcggcagaag caguccaacc ucguccccac ccagaagaag      300

cugccccagc ugagccucuu caacgaccgc uccgacgccg aguucuacca caaguacccg      360

acgaucuacc accuccggaa cgcccugaug accgagaagc gccaguucga ccuccgggag      420

aucuaccugg ccauccacca caucgugaag uaccgcggcc acuuccugaa cgccgccccc      480

gcggccagcu ucaaguccgg gaaguucgac cucgagaaca aguucgacca gcugaaccug      540

cuguucgcca gcgccuacuc cgaccuggac uucacccugc ggaccgacaa cguggaccag      600

gucaaggcga uccuccugaa cgaggaccug agcgccucca cgcgccagcg gcaggcccuc      660

ccccugaucu accagaccag cgccgaccag aaggccgcga agaagcugac caagucccuc      720

gccaccgagc ugcugaaggc cauccucggc cugaaggcca aguucgacgu gcugacgggg      780

aucgagaccg acgacaccaa ggccuggcgc cucaccuuca acgcggagga cuucgacgac      840

aagcugaacg agcugaacgu gacggacaac gaggccgcca cccagaucau cgagauccuc      900

cgcgagcugu acagcuccau ccugcucgcc gccaucgucc ccgagaacua cacccugagc      960

caggcgauga uggccaagua caacgcccac aagcagcacc ugucccuccu gcgccaggug     1020

aagaccacgc ugccggccaa gaagcagaag gagcuccagg ccgcguacga cgacuacauc     1080

gaccagcacc ucccccugga caaguucuac gccaagcuga ccaaggugcu ccccaaggag     1140

aaggacaccg acaaccccca gaucauccag auccugaacc ugaucgaccu cgaccaguuc     1200

cugccgaagc agcggaccaa ggacaacggc gccauccccc accagcugca ccagcaggag     1260

cucgaccaga ucaucgacaa ccagaaggcc uacuaccccu ggcuggcgca gcugaacccc     1320

aacgagaagc ggcagaagau cgccaaguac aagcucgacg agcuggucgc cuuccgcgug     1380

ccguacuacg uggggccccu gaucgaggcc aagacgcagc agaagaccag cggcgccaac     1440

uucgcgugga ugauccggaa ggagcccggc accaccacgc ccaucacccc guggaacuuc     1500

gacgagaagg ucaaccgcca ggccuccgcc aacgaguuca ucaagcggau gaccaccacg     1560

gacaccuacc uccuggggga ggacgugcug cccaagcaca gccuccugua ccagcgcuuc     1620

gaggugcuga acgagcucaa cggcaucaag aucgaccaga agcgggucac cgugcgccag     1680

aagcaggcca ucuucaacga ccuguuccug aagcagccca acgugaccgu caagaagcuc     1740

cagcaguacc ugcaggccaa gggggaguac gcgagcgccc ccaucaucac gggccugucc     1800

gacccgaaga aguucaacag ccagcucucc accuaccacg aguacaagaa gaucuucggc     1860

gggcugaucg acgaccccga caagcaggcc gacuucgaga agugcaucga guggagcacc     1920

aucuucgagg acucccagau ccugaaggac aagcucaagg agaucaacug gcugaccccc     1980

gagcagcggc acaagguggc caacaagcgc uaccagggcu gggggcggcu gagcaagaag     2040

cuccugaccc agcuggugga cuacaacggc cagcgcauca ucgacauccu cuggaacacg     2100

accgacaacu ucaugcagau cguccacucc gaggccuucg acaaggcgau caucaaggag     2160

aaccaggccc ugcugaccca guacaacccc caggccguga ucgacgaccu cuacaccagc     2220

ccgcagaaca agaaggcccu gcggcagauc cugcucgucg ugaacgacau ccagaaggcg     2280

cugaaggggc aggcccccuc ccgcauccug aucgaguucg cccgggaggg ccaggagaac     2340

ccccgccuca gcacgcagcg gaagcagcag gucgagaacg ccuacaagga ggccuccgac     2400

gcgcugcuga gcuccgccga ggugcgccag gagcucaagg gccuggacaa caaggcccug     2460

gccaacaccc gccucuuccu guacuucaug cagggcgggc gggacaucua caccggcaag     2520

agccugaaca ucgacgaccu cuccaaguac gacaucgacc acauccugcc gcaggccuuc     2580

aucaaggaca acagccugga caaccgcgug cucguguccg cgaagaugaa ccggagcaag     2640

uccgaccagg ucccccugaa cgacuucgcc gagauccgcu acgggaagca gaugcggcag     2700

cagugggaga agaugcugcg cgccgggcuc aucacccgga agaaguacca caaccugacg     2760

cugaaccccg agagcaucaa caaguacacc gccaagggcu ucaucaaccg ccagcucgug     2820

gagacccgcc aggugaccaa gcuggccacg aaccugcucg ucgaccagua cgcggaggac     2880

gacgugcagc ugaucaccgu caagagcaac cugacccacc agaugcggga ggaguucgac     2940

uuccccaaga accggaacgu gaacaaccac caccacgccu ucgacgccua ccucaccgcc     3000

uucgugggcc uguaccugcu caagcgcuac ccgaagcugg cgcccuacuu cguguacggg     3060

gacuaccaga agggcgccuc ccagaacagc aaguggcgga acuucaacuu ccuggggaac     3120

cuccaccgcg agaaggucgu ggacaagaac acgggcgaga ucaucuggca gcgggacgcc     3180

gggcugggcg agcugaaccg caucuacaac uucaagaaga uccucgugac ccgggcccug     3240

cgcgucaacc ggggccccau guucaagcag accaucuaca aggccgagga ggacaagucc     3300

agcgggcgcg gcuccaagca gcugaucccc accaagaaga accggccgac ggagcucuac     3360

gggggcuaca ccaacaagga gaccgcguac cugugccugg ugaaggagcc ccacaagaag     3420

ggcgacaucu accgcaucug cagcgugcuc aacauguggc ugaccgagcu ggaccgcauc     3480

gccgacccca ucgccaaggc ccagaagcuc acggagcugc ugacgcccca guucaccacc     3540

accaagaaga agcaggggca gaagaucccg gucgugaagc ccuucaaggu ggucaucccc     3600

aagauccuca ucaaccagcg guucuucucc cgcggccagg agcugaccgu ggccagcccc     3660

auguacaugc acaacgagca ggagcuguuc cucaacaaga agaccaucaa gcugcugaac     3720

gggcagcucg cgaagaacga ggcccacgag cuggccccga agaucuacca gcagauccug     3780

gaccagguga acgacuacuu cccccuguac gacauguaca aguuccggga cacccucaac     3840

gccuccaagg agaucuucgu caaccugccc uggcaggacc agcgccagga caagaagauc     3900

agccaguccg gccagaaguc cauccugaac aagauccuca ucgggcugca cgccaacgcg     3960

gcccggauca agcugacgga gcucaacuuc aagagcgacu ucggccugcu gagccagcgc     4020

uccggcaucg ccuucagccc cgacgaccag aucaucuacc aguccccgac cgggcuguuc     4080

gagcggcgcg ugacccugaa ggaccuguga                                      4110


<210>  1962
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1962
augaagguga acaacuacca caucggccug gacaucggga ccagcuccau cggcuggguc       60

gccauggggg aggacggcaa gccccuccgc gugaagggga agaccgccau cggcgcgcgg      120

cuguuccgcg agggcaaccc ggccgccgac cggcgcaugu uccggacgac ccgccggcgc      180

cugaaccggc gcaaguggcg gcucaagcug cuggacgaga ucuucgaccc cuacaucacc      240

cccguggaca gcaccuucuu cgcccgccuc aagcagucca acuggagccc caaggacucc      300

cggaaggccu ucaaggacag cacgcuguuc ccggaccuga ccgacaagca guaccaccuc      360

gacuacccca ccaucuacca ccuccgccac gcgcugauga cccaggacaa gcaguucgac      420

auccggaugg ucuaccuggc ccugcaccac aucgugaagu accgcgggaa cuuccugaac      480

uccacgcccg uggacagcuu caaggccucc aaggucaacu ucgacgacca guucaagaag      540

cucaacgagc uguacaccgc caucaacccc gaggagagcu uccagaucaa cuucgcgaac      600

uccgagaaga ucggccagca guuccuggac ccgagcaucc ggaaguucga caagaagaag      660

cagaucucca agaucgugcc cgugaccaug gacgacaagg ccgucgacaa gcucaacggg      720

aagaucgcca gcgagaucgu gaacgccauc cugggcuaca aguccaagcu ggacguggcc      780

guccagugcg accccgagga cagcaagccc ugggcgguga aguucgacga cgaggacauc      840

gacgccaagc uccagaagau ccugccggag auggacgaga accagcaguc caucaucggg      900

aucauccagg accuguacag ccaggugacc cucaaccaga ucguccccaa cggcaugucc      960

cugagcgaga gcaugaucaa gaaguacaac gaccaccacg accaccugaa gcucuacaag     1020

aagcugaucg gccagaucuc cgacagcaag aagaagacgg cccugaagaa ggccuacgac     1080

caguacgugg ggaacgacgg caaggugauc gagcaggacg acuucuacuc cagcgucaag     1140

aagaaccucg acgacuccga gcugagcaag cagaucaugg accugaucga cgccgacaag     1200

uucaugccca agcagcgcac cucccagaac ggggugaucc cccaccagcu ccaccagcgg     1260

gagcuggacg agaucaucga gcaccagagc aaguacuacc cguggcuggc ggaguccaac     1320

cccaacaagc acgaccucca ccuggccaag uacaagaucg aggagcuggu ggccuuccgc     1380

auccccuacu acgucggccc caugaucacc ccggaggacc agaagaaguc cggcgccgcc     1440

guguucagcu ggaugaagcg gaaggagccc gggcagauca cccccuggaa cuucgaccag     1500

aagguggacc gcauggagag cgcgaacaag uucaucaagc gcaugacgac caaggacacc     1560

uaccucaucg gcgaggacgu ccugcccgac gagucccugc ucuacgagaa guucaaggug     1620

cugaacgagc ugaacauggu ccgggugaac ggggacagcc ucaagguggc cgacaagcag     1680

gccaucuacc aggaccuguu cgagaacuac aagcacaucu ccgucaagaa gaugcagaac     1740

uacaucaagg acaagaccgg ccugccgaac aaccccgaca ucagcgggcu cuccgacccc     1800

gaccacuuca acaacagccu gggcacguac aacgacuuca agaagcuguu cggcgacaag     1860

guggacgacc ccgaccugca ggacgacuuc gagaagaucg uggagugguc caccgucuuc     1920

gaggacaaga agauccuccg cgccaagcug aacgagauca gcuggcugag cgacaagcag     1980

aaggacgacc ucgaguccag ccgguaccag cgcugggggc ggcuguccaa gaagcugcuc     2040

accggcaucg ugaacgacca gggggagcgc aucaucgacg agcuguggaa caccaacaag     2100

aacuucaugc agauccagag cgacgacgac uucgccaagc ggauccacga ggcgaacgcc     2160

gaccagauga aggccacgga cguggaggac guccuggccg acgcguacac cuccccgcag     2220

aacaagaagg ccauccgcca gguggugaag gucguggacg acauccagcg ggccaugggc     2280

gggguggccc ccaaguucau cagcaucgag uucacccgcu ccgaggaccg gaacccccgc     2340

cggaccauca gccgcgagcg gcagcucgag gacacgcuga aggacaccgc caagacccug     2400

gucaagucca ugaacccgga cauccucagc gagcuggaca acgcguccaa gagcaagaag     2460

ggccugaccg accgccucua ccuguacuuc acgcagcugg gccgggacau guacaccggg     2520

aagcccauca acaucgacga gaucuccaac uacgacaucg accacauccu cccccaggcc     2580

uucaucaagg acgacagccu guccaaccgc gugcugguga gcaaggccgu caacaacggc     2640

aaguccgcca acgugccccu caagcuguuc ggggugaaga uggcccacuu cuggaagcag     2700

cuggcggagg ccggccucau caacaagcgg aagcugaaga accugcagac caacccggac     2760

accaucagca aguucgccau gcacggguuc auccgccgcc agcucgucga gacgagccag     2820

gugaucaagc ugguggccaa cauccugggc gacaaguacc ggaacgacga caccaagauc     2880

aucgagauca ccgcccgcau gaaccaccag augcgggacg aguucgcguu caucaagaac     2940

cgcgagauca acgacuacca ccacgccuuc gacgccuacc ucaccgccuu ccugggccgg     3000

uaccuguacc accgcuacau caagcuccgg cccuacuucg ucuacgggga cuucaagaag     3060

uuccgcgagg acaaggugac caugcggaac uucaacuucc ugcacgaccu gacggacgac     3120

acccaggcgc gcaucgccga caccgagacc ggcgagguga ucugggaccg ggagacgucc     3180

aucaagcagc uccgcgacgu cuaccacuac aaguucaugc ugaucagcca ggagguguac     3240

acccugcgcg gggccauguu caaccagacc aucuaccccg ccgacgaggc cggccggaag     3300

aagcucaucc ccaccaaggc ggacaaggcc guggacgucu acggcgggua cuccggcagc     3360

aacgacgccu acauggccau cgugcggauc cacgacaaga agggggacaa guacaaggug     3420

gucggcgugc cgaugcgcgc ccuggaccgg cugaacgcgg ccaagaacgu guccgacgcc     3480

aaguacgacc agguccucaa ggacgugcug acgccccagu ucaccaagag caagaagucc     3540

cgcaagaccg gggagaucag ccagaccgug gaggacuucg aggucgugcu gggcaaggug     3600

auguaccggc agcucgucau cgacggcgac aagaaguuca ugcugggguc cagcacguac     3660

caguacaacg ccaagcagcu ggugcucucc gacgagagcg ugaagacccu ggccuccaag     3720

ggcaagcugg acgaggcgca ggagagcgcc gccuacgaca acgucuacga cgagauccuc     3780

gacaaggugg accgcuacuu cgcccuguac gaccagcgga acuaccgcac caaccugcac     3840

gagggggagc agaaguucau cggccucccc cugcacgaca ccuucgacgg guccaagaag     3900

guggcgaacg gcaagcgcga gacgaucagc gacauccuga acggccucca cgccaacgcc     3960

accaugacca accugaaggu ccuggggauc uccagccccu ucggcauguu ccagucccac     4020

gggccgauca accucaaccc cgagaccauc auccugugcc agagccccac gggccuguuc     4080

gagcggcgca ucauccuccg ggaccuguga                                      4110


<210>  1963
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1963
augaagaagc gcaacuacaa caucgggcug gacaucggga ccagcuccau cggcuggacc       60

aucguggacg acaacgccca gaucaugcgg gccaagggga agaacggcua cgggguccgc      120

aucuucgcgg agggcaagac ggccgccgac cggcgcggcu uccggaccac ccgccggcgc      180

cucaagcggc gcaaguggcg gcugcgccug cuccaggagu ucuucgagcc cuacauccug      240

ccgaucgacg agaacuucuu caugcggcag aaggagagca accuggugcc ccaggacccc      300

aacaagcacu ucaagggguc cauccucuuc aacgaccgca ccgaccagga guuccacgag      360

caguacccca cgaucuacca ccuccgguac gcccugauga ccgagcaccg ccaguucgac      420

gugcgggaga ucuacugggc cauccaccac aucgucaagu accgcggcca cuuccucaac      480

agcacccugg cguccaaguu caagggcggg gagcugcggc ucgccgacaa guucgagagc      540

cugaacaagc uguuccuccg cgugcaggcc gagaccgggu ucgagcuguc cgaggacaac      600

guggacaaga ucaagacggu ccugcucgac aacggcaaga gccggcugga ccgccagcgc      660

gaggugcuga agaucgugua ccucaccucc gaggacaagg acaccgagaa gaagcggaag      720

gccgucgcga ccgaguucau caaggccgug cugggccuga agacgaagcu guucaucgug      780

cuggccaaga acccgaccgc cgagaccgac uggaaguuca gcuucgacgc cgaggacauc      840

gacgacaagc uggacuccau cagcgagcag cucaccgacg acgaccacga gaucgucgag      900

cugaucuccg agauccacag caucaucacg cugaccgggg uggugcccga gggcaugggg      960

cucuccgaga gcaugaucgc gaaguacgac cagcaccagc uggaccugaa gaagcucaag     1020

ucccugaugc gcgaccugga cgucaagcag gcccggaagc uccaggacgc cuacgacgag     1080

uacaucgacg gccugaagcc cagcgaggag gccuucuacu ccgccgugag cagcgugauc     1140

cagaagaugg acgacccccg cgcgaagaag aucaccgccg agaucgagcu ggaggaguuc     1200

augccgaagc agcggaccaa ggccaacggc cagauccccc accagcucca ccagcaggag     1260

cuggaccaga ucaucgagaa ccagaaggag uacuaccccu ggcuggccga ggagaacccc     1320

aacaagcgcg agcacaacgc cgcgccgcac aagcucgacg ggcuggucac guuccgggug     1380

cccuacuacg ugggcccccu gaucgaggcc uccguccagc agaagaccag caacuccaac     1440

uucgccugga uggagcgcaa ggugaagaac gacaccagcc ggaucacccc cuggaacuuc     1500

gaggagaagg uggaccgcaa cgccuccgcc gagaacuuca ucaagcggau gacgaccaag     1560

gacaccuacc uccugaacga ggacguccug ccgcagcaga gccuccugua ccagcgcuac     1620

gaggugcuga acgagcugaa caacguccgg gugaacgaca agaagcucga gaacaacugc     1680

aagcaggacg uguacaagga ccuguucaag aacaacacca cggucuccau caagaaguuc     1740

aaggacuacc ugguggcgaa cugcgaguac gccgacaacc ccaagaucga ggggcucgcc     1800

gacgagagca ucuuccuguc cagccugucc accgagaaca aguucaagga caucuucggc     1860

gcccagaucg acgaccccaa cuacuucgag gacuucgagc acaucaucga gugggcgacc     1920

aucuucgagg acaacaagau ccuccgcgcc aagcuggacg agaucagcug gcugaacgag     1980

ucccagaaga aggagcucgc caagcugcgg augcgcgggu ggggccggcu gagccgcaag     2040

cucaucgccg agaucaccga cgagaacggc ucccggauca ucgaccugcu gugggagcac     2100

auggacaacu ucaugguggu cgugaaccag gacguguuca aggagaagau cgucgccgag     2160

aacaagcagg ucuucaagga gcucaacccc cuggacauca ucaacgagca guucacgagc     2220

ccgcagaaca agaaggcgcu gcgccagauc cuccuggugg uggccgacau ccagaaggcc     2280

augaacgggg ugacccccuc caacaucuuc aucgaguucg cccgcgagga cgagcggaac     2340

ccccgccgga gcauccagcg ccugcgccag guggagaaca uguaccggga caucuccgac     2400

accguccucg ccgaccccga ggugcggaac gagcugagca acaucaagaa caccgagcug     2460

gcgcgcgacc ggguguuccu cuacuuccug cagggcgggc gcgacgucua cacgggcgag     2520

ccgcugaaca ucgacaagcu cagcgaguac gacaucgacc acauccugcc ccaggccuuc     2580

aucaaggaca acucccugga caaccgggug cucgugagcg ccucccugaa ccgcagcaag     2640

uccgacagcg ucccccugga cgacuucacc uccgagaugu ucgggcggaa gauggagccc     2700

cucugggagc agaugcagcg cgccggccug aucagcaaga agaagcuggc caaccucaag     2760

uucaacccgg agaacaucaa caaguacacc cggaacggcu ucaucaaccg ccagcuggug     2820

gagacccgcc aggugaucaa gcuggcggcc aacauccuca aggaccacua cggggaggag     2880

accgagaucg ucaacgugaa ggccggccug acgcaccagu uccggaagga cuucgacuuc     2940

cccaagaacc ggaacgugaa caacuaccac cacgccuucg acgcguaccu gaccgcccuc     3000

gucgggaccu accugcugaa gaaguacccc aagcucgagu ccuacuucgu guacggcaag     3060

uucaagaagg gggacggcau caccaaccug aagaacuuca acuuccugca cggcuucaac     3120

aagccgggga agaucaucaa caaggagacg ggcgagaucc ucuucgaccg cgacaagcag     3180

cugggguaca ugaacagcau cuacgaccug cggaagguga ucgucaccca cgagcucucc     3240

acccgccacg gcgccaugua cgaccagacc cuguacaagg ccagcgacga caaggacucc     3300

ggccgcgggg gcaagcagcu gauccggaag aaggacaacc uccccacgga gaucuacggg     3360

ggcuacaccg ggagcaccga cgccuucaug uccaucaucc gcaugaagaa gaaggacaag     3420

cuguacuaca aggugaucgg cgugcccacc cgggucgcgc acgaggugcu gcagagcgac     3480

ggcaagauca acaucuucac gcucaacaag uucaucgagc agaagcugac caaggagaag     3540

gugaacaaga agaccggggc caucacccgc aucacggacu gguucgagcu ggucaucgcc     3600

aaggugaugu acggccaggu gaucgacgac gggggccagc ccuucaugcu cggguccagc     3660

acccugccgu acaacguccg ggagcuguac cucgccaagg accuggugaa ggugaucaac     3720

aagggcagca ccuccaagag cuccuaccug gacgucuacg acgagcucgu gaagucccug     3780

aacaaguacu uccccaugua cgaccgcaac auguuccggc agaagaucac cgacgcccgc     3840

gacaaguucg agaagcugcc caagcgggac aaggugagca acggcaagcu ccagcagauc     3900

gggcagguca cgaucaucga ccgcauccug aucggccugc acaccaacgc gggggugagc     3960

aaccucaagg accugggcau cgccaccccc uucggguuca ugcagcaggg caacggcauc     4020

ucccugacca gcacggccaa gaucaucuac caguccagca ccggccucuu cgagaagucc     4080

aaggcccugg acgaccugaa cccgcucgcc uga                                  4113


<210>  1964
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1964
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgaugcagg aggagaagua cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccuca ucuaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgacc gcuucgacgu gcggaacacc      540

gacauccaga agcaguacca ggcguuccug gagaucuucg acaccacguu cgagaacaac      600

gaccugcucu cccagaacgu ggacgucgag gccauccuga ccgacaagau cagcaagucc      660

gccaagaagg accgcauccu ggcccgguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gcggacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucagcgccg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggucacc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggcguucu acaaguaccu gagcaagcuc     1140

cugacgaagc aggagggguc cgaguaccug cucgagaaga ucaagaacga ggacuuccug     1200

cgcaagcagc gcaccuucga caacggcagc aucccccacc aggugcaccu gaccgagcuc     1260

cgggccauca uccgccggca guccgaguac uacccguucc ugaaggagaa ccuggaccgc     1320

aucgagaaga uccucaccuu ccggaucccc uacuacgucg gcccccuggc ccgcgagaag     1380

uccgacuucg ccuggaugac gcggaagacc gacgacagca uccgccccug gaacuucgag     1440

gaccuggugg acaaggagaa guccgccgag gcguucaucc accggaugac caacaacgac     1500

cuguaccucc cggaggagaa ggugcugccc aagcacagcc ugaucuacga gaaguucacc     1560

gucuacaacg agcucacgaa ggugcgcuuc cuggccgagg gguucaagga cuuccaguuc     1620

cugaaccgga agcagaagga gaccaucuuc aacagccucu ucaaggagaa gcgcaaggug     1680

accgagaagg acaucaucuc cuuccugaac aaggucgacg gcuacgaggg gaucgccauc     1740

aagggcaucg agaagcaguu caacgccagc cuguccaccu accacgaccu caagaagauc     1800

cuggggaagg acuuccugga caacacggac aacgagcuca uccuggagga caucgugcag     1860

acccugaccc ucuucgagga ccgggagaug auccgcaagc ggcuggacau cuacaaggac     1920

uucuucaccg agagccagcu gaagaagcuc uaccgccggc acuacacggg cuggggccgc     1980

cuguccgcga agcugaucaa cgggauccgg aacaaggaga accagaagac cauccucgac     2040

uaccugaucg acgacggcag cgccaaccgc aacuucaugc agcugaucaa ggacgccggg     2100

cucuccuuca agcccaucau cgacaaggcc cgcaccggca gccacuccga caaccugaag     2160

gagguggucg gggagcuggc cggcagcccc gcgaucaaga agggcauccu ccagucccug     2220

aagaucgugg acgagcuggu gaaggucaug ggguacgagc cggagcagau cguggucgag     2280

auggcccggg agaaccagac cacggccaag ggccucagcc gccugcggca gcgccugacc     2340

acccuccggg agagccuggc caaccugaag uccaacaucc ucgaggagaa gaagcccaag     2400

uacgugaagg accaggugga gaaccaccac cugagcgacg accgccuguu ccucuacuac     2460

cugcagaacg ggaaggacau guacaccgac gacgagcugg acaucgacaa ccucucccag     2520

uacgacaucg accugaucau cccccaggcc uucaucaagg acgacagcau cgacaaccgg     2580

guccuggugu ccagcgcgaa gaaccgcggc aaguccgacg acgugcccag cgucgagauc     2640

gugaaggacu gcaagguguu cuggaagaag cuccuggacg ccaagcugau gucccagcgg     2700

aaguacgaca accucaccaa ggccgagcgc gggggccuga cgagcgacga caaggcccgg     2760

uucauccagc gccagcuggu cgagacccgg cagaucacca agcacguggc ccgcauccuc     2820

gacgagcggu ucaacaacga gcuggacucc aagggccgcc ggauccgcaa ggugaagauc     2880

gucacccuca agagcaaccu cguguccaac uuccggaagg aguucggguu cuacaagauc     2940

cgcgagguga acaacuacca ccacgcgcac gacgccuacc ugaacgccgu cguggccaag     3000

gcgauccuga cgaaguaccc gcagcucgag cccgaguucg uguacggcga cuaccccaag     3060

uacaacagcu acaagacccg gaaguccgcc accgagaagc uguucuucua cagcaacauc     3120

augaacuucu ucaagaccaa ggucacgcug gccgacggga ccgugguggu caaggacgac     3180

aucgagguga acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

guccgcaagg ugcucuccua cccccaggug aacaucguca agaagacgga gauccagacc     3300

ggcggguuca gcaaggagag cauccuggcc cacggcaacu ccgacaagcu gaucccgcgc     3360

aagaccaagg acaucuaccu cgaccccaag aaguacgggg gcuucgacag ccccaucgug     3420

gcguacuccg ugcuggucgu ggccgacauc aagaagggga aggcccagaa gcugaagacc     3480

gugacggagc uccugggcau caccaucaug gagcggagcc gcuucgagaa gaaccccucc     3540

gccuuccugg agagcaaggg cuaccucaac auccgggacg acaagcugau gauccugccg     3600

aaguacuccc ucuucgagcu ggagaacggg cgccggcgcc ugcucgccag cgcggacgag     3660

cugcagaagg gcaacgagcu ggcccucccc acccaguuca ugaaguuccu guaccuggcc     3720

ucccgguaca acgagcucaa ggggaagccc gaggagaucg agcagaagca ggaguucguc     3780

gugcagcacg ugagcuacuu cgacgacauc cugcagauca ucaacgacuu cuccaaccgc     3840

gucauccugg ccgacgccaa ccucgagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucccgg uggacgagcu ggcgaacaac aucaucaacc ucuucaccuu cacgagccug     3960

ggcgcccccg ccgccuucaa guucuucgac aagaucgugg accggaagcg cuacaccucc     4020

accaaggagg uccugaacag cacccucauc caccagucca ucacggggcu guacgagacc     4080

cggaucgacc ugggcaagcu cggcgaggac uga                                  4113


<210>  1965
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1965
augaccaaga agaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucacgg acgacuacaa ggugcccgcc aagaagauga aggugcucgg gaacaccgac      120

aagaaguaca ucaagaagaa ccugcugggc gcgcuccugu ucgacagcgg ggagaccgcc      180

gaggccaccc gccugaagcg gacggcccgc cggcgcuaca cccggcgcaa gaaccggcuc      240

cgcuaccugc aggagaucuu cgccgaggag augaccaagg ucgacgaguc cuucuucuac      300

cggcuggacg agagcuuccu caccacggac gagaaggacu ucgagcgcca cccgaucuuc      360

ggcaacaagg cggaggagga cgccuaccac cagaaguucc ccaccaucua ccaccuccgg      420

aacuaccugg ccgacuccag cgagaaggcc gaccuccgcc ugguguaccu ggcgcucgcc      480

cacaugauca aguaccgggg ccacuuccug aucgagggga agcugaacgc cgagaacacc      540

gacgugcaga agcuguucac cgacuucguc ggcguguacg accgcacguu cgacgacucc      600

caccugagcg agaucaccgu ggacgucgcc uccacccuga ccgagaagau cagcaagucc      660

cggcgccucg agaaccugau caaguacuac cccacggaga agaagaacac ccuguucggg      720

aaccucaucg cccuggcgcu gggccuccag cccaacuuca agaugaacuu caagcugagc      780

gaggacgcca agcugcaguu cuccaaggac accuacgagg aggaccucga ggagcugcug      840

gggaagaucg gcgacgacua cgccgaccuc uucaccagcg ccaagaaccu guacgacgcc      900

auccugcucu ccggcauccu gacgguggac gacaacagca ccaaggcgcc gcuguccgcc      960

agcaugauca agcgguacgu ggagcaccac gaggaccucg agaagcugaa ggaguucauc     1020

aaggccaaca aguccgagcu guaccacgac aucuucaagg acaagaacaa gaacggguac     1080

gccggcuaca ucgagaacgg ggucaagcag gacgaguucu acaaguaccu caagaacacc     1140

cugagcaaga ucgacggcag cgacuacuuc cuggacaaga ucgagcgcga ggacuuccuc     1200

cggaagcagc gcaccuucga caacggcucc aucccccacc agauccaccu ccaggagaug     1260

cacgccaucc ugcggcgcca gggggacuac uaccccuucc ucaaggagaa gcaggaccgc     1320

aucgagaaga uccugacguu ccggaucccc uacuacgugg gcccgcuggu gcgcaaggac     1380

agccgguucg cgugggccga guaccgcucc gacgagaaga ucacccccug gaacuucgac     1440

aaggucaucg acaaggagaa guccgccgag aaguucauca cccggaugac ccugaacgac     1500

cucuaccugc ccgaggagaa ggugcugccc aagcacagcc acguguacga gacguacgcc     1560

gucuacaacg agcucaccaa gaucaaguac gugaacgagc aggggaagga gagcuucuuc     1620

gacuccaaca ugaagcagga gaucuucgac cacguguuca aggagaaccg caaggucacc     1680

aaggagaagc ugcugaacua ccucaacaag gaguucccgg aguaccggau caaggaccug     1740

aucggccugg acaaggagaa caagagcuuc aacgccuccc ucgggaccua ccacgaccug     1800

aagaagaucc uggacaaggc guuccucgac gacaagguga acgaggaggu gaucgaggac     1860

aucaucaaga cgcugacccu guucgaggac aaggacauga uccacgagcg ccuccagaag     1920

uacagcgaca ucuucaccgc caaccagcug aagaagcugg agcggcgcca cuacaccggc     1980

uggggccggc ucuccuacaa gcugaucaac gggauccgca acaaggagaa caacaagacg     2040

auccuggacu accucaucga cgacggcagc gccaaccgga acuucaugca gcugaucaac     2100

gacgacaccc ugcccuucaa gcagaucauc cagaaguccc aggucguggg ggacgucgac     2160

gacaucgagg ccguggugca cgaccucccc ggcagccccg cgaucaagaa ggggauccug     2220

caguccguca agaucgugga cgagcuggug aaggucaugg gcgacaaccc ggacaacauc     2280

gugaucgaga uggcccgcga gaaccagacc accaaccggg gccgcagcca gucccagcag     2340

cggcucaaga agcugcagag cucccugaag gagcucggga gcaacauccu gaacgaggag     2400

aagcccagcu acaucgagga caagguggag aacucccacc uccagaacga ccagcuguuc     2460

cuguacuaca uccagaacgg caaggacaug uacaccgggg acgagcugga caucgaccac     2520

cugagcgacu acgacaucga ccacaucauc ccccaggccu ucaucaagga cgacuccauc     2580

gacaaccgcg uccugacgag cuccgccaag aaccggggca agagcgacga cgugcccucc     2640

cuggacaucg ugcgcgcccg caaggcggag uggguccggc ucuacaagag cgggcugauc     2700

uccaagcgca aguucgacaa ccugaccaag gccgagcggg gcggccucac cgaggccgac     2760

aaggccgggu ucaucaagcg ccagcuggug gagacccggc agaucacgaa gcacguggcc     2820

cagauccugg acgcgcgcuu caacaccgag cacgacgaga acgacaaggu cauccgggac     2880

gugaagguga ucacccucaa gagcaaccug gucucccagu uccgcaagga cuucgaguuc     2940

uacaaggugc gggagaucaa cgacuaccac cacgcccacg acgccuaccu gaacgccgug     3000

gucggcaccg cccuccugaa gaaguacccg aagcuggcga gcgaguucgu guacggggag     3060

uacaagaagu acgacauccg caaguucauc acgaacucca gcgacaaggc caccgccaag     3120

uacuucuucu acuccaaccu caugaacuuc uucaagacca aggugaagua cgccgacggc     3180

accgucuucg agcggcccau caucgagacg aacgcggacg gcgagaucgc cuggaacaag     3240

cagaucgacu ucgagaaggu gcgcaaggug cugagcuacc cccaggucaa caucgugaag     3300

aagguggaga cccagaccgg gggcuucucc aaggagagca uccugcccaa gggggacagc     3360

gacaagcuca ucccgcggaa gaccaagaag gucuacuggg acacgaagaa guacggcggg     3420

uucgacuccc ccaccguggc cuacagcgug uucgucgugg ccgacgugga gaagggcaag     3480

gccaagaagc ugaagaccgu caaggagcug gugggcaucu ccaucaugga gcgcagcuuc     3540

uucgaggaga accccgugga guuccucgag aacaaggggu accacaacau ccgggaggac     3600

aagcugauca agcugcccaa guacucccuc uucgaguucg agggcgggcg ccggcgccug     3660

cuggcgagcg ccuccgagcu ccagaagggc aacgagaugg uccugccggg guaccuggug     3720

gagcuccugu accacgccca ccgggccgac aacuucaaca gcaccgagua ccugaacuac     3780

guguccgagc acaagaagga guucgagaag guccucagcu gcguggagga cuucgccaac     3840

cuguacgugg acgucgagaa gaaccugucc aagauccgcg cgguggccga cagcauggac     3900

aacuucucca ucgaggagau cagcaacucc uucaucaacc uccugacgcu gaccgcccuc     3960

ggcgcccccg ccgacuucaa cuuccugggc gagaagaucc cccggaagcg cuacaccagc     4020

accaaggagu gccugacggc gacccucauc caccagucca ucaccgggcu guacgagacc     4080

cgcaucgacc ugagcaagcu cggcgaggag uga                                  4113


<210>  1966
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1966
augaagaacg ccaacaaggu gaccggcaag uacuaccugg gguucgacau cggcaccaac       60

agcgucgggu gggccgugac ggacgagaac uacaaccucu gcaaguucaa gaagaagucc      120

auguggggca uccgccuguu cgaggacgcg aagaccgccg aggagcggcg cgggcagcgg      180

gccagccgcc ggcgccugca gcggcgcaag cagcggaucg accuccugca ggagaucuuc      240

gccgaggaga ucaacaaggu ggacgagacc uucuucaucc gccugaacga gucccggcuc      300

caccuggacg acaagaccgu cagcgagaag cacccccugu ucaucggcuc cgaguacagc      360

gagaaggagu acuaccgcga guacccgacg aucuuccacc uccggaagga gcugauggag      420

uccagcgagc cccacgacau ccgccuggug uaccucgcca uccaccacau ccugaagaac      480

cggggccacu uccugaucga cggggagcuc uccaaggcga acagcuucga cgccgccuuc      540

uaccagauga aggaggugcu gugcaacgag cuggaccucg agaucgacgg cgagaacacc      600

accgaggucg agcgcacccu gcgggaccgc aacaucucca agagcgacaa ggugaagcug      660

cucgccgcgc ugcuggccau gaaccagaac gagacggagu ccaaggagac caagaagaag      720

aucgagaaca ucugcaagcu ccuggccggg aacaagggcg acaucagcaa gaucuucggg      780

cagacccccg agggcgugga gaagaccucc uucagcuucg gcgaggccga cuacgacgag      840

uccauccggc ccaaccugga ggagagcauc ccggaccagu gcuucgucau cgacuccauc      900

aaggcccucu acgacuggaa cgugcuggcg gacauccuga acgggcagac guaccucagc      960

ugcgccaagg ugaaccagua cgagacccac aagaacaacc ugaaggagcu gaagcuccug     1020

gucaaccgcu acugcagccg ggaggaguac aagcgcuucu ucaaguccgc cguggagaag     1080

gccaacuaca gcaacuacau cggcuccgcc aagcagggga acaagaucau cgacgugaag     1140

cgcugcgcgg aggaggacuu cuacaaggag cugaagaucc uccugagcaa gaucgagccc     1200

gaggaggccg accgggagcu guacgagcgc aucaagcggg aggccgagga gggcucccuc     1260

cugccccugc agcgcaacaa ggacaacagc gucgugccca accagaugca caaggccgag     1320

cucgcccuga uccuggagaa cgcgggcucc uaccucgggu uccugaacga gaaggacgag     1380

gacggccuga gcccggccga gaagaucauc gagcuguucu ccuuccggau ccccuacuac     1440

guggggcccc ugagcgaccg ccacaaggac cggggcucca acgccuggau cguccgcaag     1500

gccgaggggc ggauccugcc cuggaacuuc gacgagaagg uggaccgcga gaaguccaac     1560

gaggccuuca ucgagcggau gaccaacaag ugcaccuacc ugcucggcaa ggacgugguc     1620

gcgaagaaca gccugaucua cagccacuuc auggugcuga acgagcucaa caaccugucc     1680

gugugcggca acaagaucag cguccaggug aagcaggaca ucuaccgcga gcuguuccag     1740

cggcacacga aggucuccaa gaagaugcuc cugcaguacu gccgcgagaa gaugaugaac     1800

aaggaccugg aggaggagga ccucagcggg uucgacggcg acuucaaggc caagcugucc     1860

agcuaccugg acuucgagaa gaagguguuc ggggccaaga uggaggagga ccggauccgc     1920

aagaucaccg aggacaucau ccgguggaag accaucuacg gcgacgacgg gaagaugcuc     1980

caggccgugg ucgagaagga guacccgggc gagcuggaca gcgaccagcu gaaggcggug     2040

aagcaguucc gcuacuccgg cugggggaac uucagcgagg aguuccucaa cggcaucgug     2100

gggaccgaga agcccacggg ccagauguuc accaucaucg aggcccugug gcagaccaac     2160

ugcaaccuga ugcagcuccu guccaagaac uucaccuuca uggaggagau cgagaagcac     2220

aaccgggagc uggccgggca gaucacggac aucagcuacg acaagcucgu caaggaccug     2280

uacaucuccc ccgccaacaa gcgcgccgug uggcagaccg ugcgcaucgc ggaggagguc     2340

aagaagauca ugggcugcga gcccgccaag aucuucgugg agauggcccg gggcggggag     2400

aaggagaaga agcgcaccau cagccggaag aagaagcugc ucgagcugua cgccgccugc     2460

aagaaggacg cgcgcgacug gaaccgggag aucgaggacc gcgacgagcg ggaguucaac     2520

uccgacaagc uguaccucua cuacacccag augggccgcu gcauguacag cggggagcgg     2580

aucgaccugc acgagcugau guccgccaac agcaaguggg acaucgacca caucuacccg     2640

caguccaagg ugaaggacga cagccucgac aaccgcgucc ugucccugaa gaagaucaac     2700

aacgagaagg gcaacaagcc ccucagccgg gacauccagc agucccagca gaaguggugg     2760

acggagcugc ugcagcucga guucaucacc aagaagaagu acgaccgccu gaugcaccgg     2820

ggcgagcuga gcgaggagga gcucuccggg uucaucaacc gccagcuggu ggagacccgg     2880

cagagcacca aggccgccgc cgagcugcuc ggccgcaugu acaacaacag caaggugguc     2940

uacgugaagg cgccccuggu gucccaguuc cggaaggacg accugaacau ccucaagagc     3000

cgccuggcca acgacuacca ccacgccaag gacgccuacc ugaacaucgc ggucgggaac     3060

guguacaacg agaaguucac ggacaacccc cuccggugga ucgagaagca cgccggcgag     3120

aacuacucca ucaaccgcgu guucgacuac gacaucgucg ccggggacgg cgccccggug     3180

ugggccgcgc cccugcggga cgagaagaac aagcugcgca agaacgaggu gggcgagaag     3240

uacgggggca ccaucgacgu cauccgcaag accgugaagc ggaacgacgu gcucuacacc     3300

gagcacacgu acugcgggaa gggcgagcug uucaacgcca ccaucgccaa gaaggacggg     3360

ggcgccgaga ucccccugaa ggccggccuc cagaccggga aguacggcgg guacaagagc     3420

cccaacaccu ccuacuucgc ggccaucgag uucgacggca agaaggggga gcgcgucaag     3480

aacaucaugg aggugccggu guacaucgcc aacauccugc ugcacgacau cgacgccuac     3540

auccgguacu gcgagaccgu caagggccuc aagaacguga agauccugcg ccccugcauc     3600

aagaagaacg cccugaucag cguggacggc uaccccaugc ggauccgcgg ggcgaacgag     3660

aagaacaucc uccugaagaa caaccugcag cccaucuucg gccggcacga ggagacgguc     3720

cgccggaucg agaaguacgu ggagaagaac caggaguucg agguggacga gaaguucgac     3780

gggcucaacg aggccaaccu gaccgaccug uacgacgaca ucaccgagaa gcucugcggc     3840

ccguacgucg agcgccccuc caaccagggg gaguaccuga uggagaaccg ggagaaguuc     3900

cugaacauca gccucaagga gaaggccaag cugcuggccc agaugaucaa gcucgugcgc     3960

ugcgacaccg agacgaaggc ggaccugcgg uccaucggcg ccagcaagaa cgccggcgug     4020

aucuccguca acaagaacac ccucgggggc aagcgccuca uccugaucaa ccagagcgug     4080

accgggcugu ucgagaaccg gaucgagcuc uga                                  4113


<210>  1967
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1967
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgaugcagg aggagaagua cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccuca ucuaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgacc gcuucgacgu gcggaacacc      540

gacauccaga agcaguacca ggcguuccug gagaucuucg acaccacguu cgagaacaac      600

gaccugcucu cccagaacgu ggacgucgag gccauccuga ccgacaagau cagcaagucc      660

gccaagaagg accgcauccu ggcccgguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gcggacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucagcgccg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggucacc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggcguucu acaaguaccu gagcaagcuc     1140

cugacgaagc aggagggguc cgaguaccug cucgagaaga ucaagaacga ggacuuccug     1200

cgcaagcagc gcaccuucga caacggcagc aucccccacc aggugcaccu gaccgagcuc     1260

cgggccauca uccgccggca guccgaguac uacccguucc ugaaggagaa ccuggaccgc     1320

aucgagaaga uccucaccuu ccggaucccc uacuacgucg gcccccuggc ccgcgagaag     1380

uccgacuucg ccuggaugac gcggaagacc gacgacagca uccgccccug gaacuucgag     1440

gaccuggugg acaaggagaa guccgccgag gcguucaucc accggaugac caacaacgac     1500

cuguaccucc cggaggagaa ggugcugccc aagcacagcc ugaucuacga gaaguucacc     1560

gucuacaacg agcucacgaa ggugcgcuuc cuggccgagg gguucaagga cuuccaguuc     1620

cugaaccgga agcagaagga gaccaucuuc aacagccucu ucaaggagaa gcgcaaggug     1680

accgagaagg acaucaucuc cuuccugaac aaggucgacg gcuacgaggg gaucgccauc     1740

aagggcaucg agaagcaguu caacgccagc cuguccaccu accacgaccu caagaagauc     1800

cuggggaagg acuuccugga caacacggac aacgagcuca uccuggagga caucgugcag     1860

acccugaccc ucuucgagga ccgggagaug auccgcaagc ggcuggacau cuacaaggac     1920

uucuucaccg agagccagcu gaagaagcuc uaccgccggc acuacacggg cuggggccgc     1980

cuguccgcga agcugaucaa cgggauccgg aacaaggaga accagaagac cauccucgac     2040

uaccugaucg acgacggcag cgccaaccgc aacuucaugc agcugaucaa ggacgccggg     2100

cucuccuuca agcccaucau cgacaaggcc cgcaccggca gccacuccga caaccugaag     2160

gagguggucg gggagcuggc cggcagcccc gcgaucaaga agggcauccu ccagucccug     2220

aagaucgugg acgagcuggu gaaggucaug ggguacgagc cggagcagau cguggucgag     2280

auggcccggg agaaccagac cacggccaag ggccucagcc gcagccggca gcgccugacc     2340

acccugcggg agucccucgc caaccucaag agcaacaucc uggaggagaa gaagcccaag     2400

uacgugaagg accaggugga gaaccaccac cucuccgacg accgccuguu ccuguacuac     2460

cuccagaacg ggaaggacau guacaccgac gacgagcugg acaucgacaa ccugagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacuccau cgacaaccgg     2580

guccucguga gcuccgcgaa gaaccgcggc aagagcgacg acgugcccuc cgucgagauc     2640

gugaaggacu gcaagguguu cuggaagaag cugcuggacg ccaagcucau gagccagcgg     2700

aaguacgaca accugaccaa ggccgagcgc gggggccuga cguccgacga caaggcccgg     2760

uucauccagc gccagcucgu cgagacccgg cagaucacca agcacguggc ccgcauccug     2820

gacgagcggu ucaacaacga gcuggacagc aagggccgcc ggauccgcaa ggugaagauc     2880

gucacccuca aguccaaccu ggugagcaac uuccggaagg aguucggguu cuacaagauc     2940

cgcgagguga acaacuacca ccacgcgcac gacgccuacc ugaacgccgu cguggccaag     3000

gcgauccuca cgaaguaccc gcagcuggag cccgaguucg uguacggcga cuaccccaag     3060

uacaacuccu acaagacccg caagagcgcc accgagaagc uguucuucua cuccaacauc     3120

augaacuucu ucaagaccaa ggucacgcuc gccgacggga ccgugguggu caaggacgac     3180

aucgagguga acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

guccggaagg ugcugagcua cccccaggug aacaucguca agaagacgga gauccagacc     3300

ggcggguuca gcaaggaguc cauccuggcc cacggcaaca gcgacaagcu caucccgcgc     3360

aagaccaagg acaucuaccu ggaccccaag aaguacgggg gcuucgacuc ccccaucgug     3420

gcguacagcg ugcuggucgu ggccgacauc aagaagggga aggcccagaa gcucaagacc     3480

gugacggagc ugcugggcau caccaucaug gagcgguccc gcuucgagaa gaaccccagc     3540

gccuuccucg aguccaaggg cuaccugaac auccgggacg acaagcugau gauccucccg     3600

aaguacagcc uguucgagcu ggagaacggg cgccggcgcc uccuggccuc cgcgggcgag     3660

cugcagaagg ggaacgagcu cgcccugccc acccaguuca ugaaguuccu guaccucgcc     3720

agccgguaca acgagcugaa gggcaagccc gaggagaucg agcagaagca ggaguucguc     3780

gugcagcacg uguccuacuu cgacgacauc cugcagauca ucaacgacuu cagcaaccgc     3840

gucauccucg ccgacgccaa ccuggagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucccgg uggacgagcu cgcgaacaac aucaucaacc uguucaccuu cacgucccug     3960

ggggcccccg ccgccuucaa guucuucgac aagaucgugg accggaagcg cuacaccagc     4020

accaaggagg uccucaacuc cacccugauc caccagagca ucacgggccu guacgagacc     4080

cggaucgacc ucggcaagcu gggggaggac uga                                  4113


<210>  1968
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1968
augaccaaga ucaaggacga cuacaucgug ggccuggaca ucgggaccga cagcugcggc       60

ugggucgcca ugaacuccaa caacgacauc cucaagcugc aggggaagac ggccaucggc      120

agccgccugu ucgagggcgg gaaguccgcg gccgagcggc gccucuuccg gaccacccac      180

cgccggauca agcgccggcg cuggcggcug aagcugcucg aggaguucuu cgaccccuac      240

auggccgagg uggacccgua cuucuucgcc cgccugaagg agagcggccu gagcccccuc      300

gacaagcgga agaccgugag cuccaucguc uuccccacga gcgccgagga caagaaguuc      360

uacgacgacu accccaccau cuaccaccuc cgcuacaagc ugaugaccga ggacgagaag      420

uucgaccucc gggaggugua ccuggcgauc caccacauca ucaaguaccg cgggaacuuc      480

cuguacaaca ccuccgugaa ggacuucaag gccagcaaga ucgacgucaa guccagcauc      540

gagaagcuca acgagcugua cgagaaccug ggccucgacc ugaacgugga guucaacauc      600

uccaacacgg ccgagaucga gaaggugcug aaggacaagc agaucuucaa gcgggacaag      660

gucaagaaga ucgccgagcu guucgcgauc aagaccgaca acaaggagca gagcaagcgc      720

aucaaggaca ucuccaagca ggucgccaac gccgugcugg gguacaagac ccgguucgac      780

accaucgccc ugaaggagau cagcaaggac gagcucuccg acuggaacuu caagcugagc      840

gacaucgacg ccgacuccaa guucgaggcg cugaugggca accucgacga gaacgagcag      900

gccauccugc ugacgaucaa ggagcuguuc aacgagguga cccucaacgg gaucguggag      960

gacggcaaca cccugagcga gagcaugauc aacaaguaca acgaccaccg cgacgaccuc     1020

aagcugcuga aggaggugau cgagaaccac aucgaccgga agaaggccaa ggagcucgcc     1080

cuggccuacg accuguacgu caacaaccgc cacggccggc uccugcaggc gaagaagaag     1140

cuggggaaga ucaagccgcg cuccaaggag gacuucuaca agguggugaa caagaaccuc     1200

gacgacagcc gcgccuccaa ggagaucaag aagaagaucg agcuggacag cuucaugccc     1260

aagcagcgga ccaacgccaa cggcgucauc cccuaccagc ugcagcagcu cgagcuggac     1320

aagaucaucg agaaccaguc caaguacuac cccuuccuga aggagaucaa cccggugagc     1380

ucccaccuca aggaggcccc cuacaagcug gacgagcuga uccgcuuccg ggugcccuac     1440

uacgucgggc cccugaucag cccgaacgag uccacgaagg acauccagac caagaagaac     1500

cagaacuucg ccuggaugau ccgcaaggag gagggccgga ucacccccug gaacuucgac     1560

cagaaggugg accgcaucga gagcgcgaac aaguucauca agcggaugac cacgaaggac     1620

accuaccucu ucggcgagga cgugcugccc gccaacuccc ugcucuacca gaaguucacc     1680

guccugaacg agcugaacaa cauccgcauc aacgggaagc ggaucagcgu ggaccucaag     1740

caggagaucu acgagaaccu guucaagaag cacaccacgg ucaccgugaa gaagcuggag     1800

aacuaccuca aggagaacca caaccuggug aaggucgaga ucaagggccu ggccgacgag     1860

aagaaguuca acuccgggcu caccaccuac aaccgcuuca agaaccugaa caucuucgac     1920

aaccagaucg acgaccugaa guaccggaac gacuucgaga agaucaucga guggagcacg     1980

aucuucgagg acaaguccau cuacaaggag aagcuccgca gcaucgacug gcugaacgag     2040

aagcagauca acgcccuguc caacauccgg cuccagggcu gggggcgccu gagcaagaag     2100

cugcucgccc agcugcacga ccacaacggc cagaccauca ucgagcagcu gugggacucc     2160

cagaacaacu ucaugcagau cgugacccag gcggacuuca aggacgccau cgccaaggcc     2220

aaccagaacc uccugguggc gaccagcguc gaggacaucc ugaacaacgc cuacacgagc     2280

cccgccaaca agaaggccau ccgccaggug aucaaggugg ucgacgacau cgugaaggcc     2340

gcguccggca aggugccgaa gcagaucgcc aucgaguuca cccgggacgc cgacgagaac     2400

cccaagcgga gccagacccg cggguccaag cuccagaagg ucuacaagga ccugagcacc     2460

gagcuggccu ccaagacgau cgccgaggag cucaacgagg cgaucaagga caagaagcug     2520

gugcaggaca aguacuaccu guacuucaug cagcucggcc gggacgccua caccggggag     2580

cccaucaaca ucgacgagau ccagaaguac gacaucgacc acauccugcc ccagagcuuc     2640

aucaaggacg acgcccugga caaccgcgug cucgucuccc gcgccgugaa caacggcaag     2700

agcgacaacg ugccggucaa gcuguucggg aacgagaugg ccgcgaaccu gggcaugacc     2760

auccggaaga ugugggagga guggaagaac aucggccuca ucuccaagac caaguacaac     2820

aaccugcuga ccgaccccga ccacaucaac aaguacaaga gcgccggguu cauccgccgg     2880

cagcucgugg agacguccca gaucaucaag cuggugagca ccauccugca gucccgcuac     2940

cccaacaccg agaucaucac cgucaaggcc aaguacaacc acuaccuccg ggagaaguuc     3000

gaccuguaca agagccgcga ggugaacgac uaccaccacg ccaucgacgc guaccugucc     3060

gccaucugcg gcaaccuccu guaccagaac uaccccaacc ugcggccguu cuucguguac     3120

gggcaguaca agaaguucag cuccgacccc gacaaggaga aggagaucuu caacaagacg     3180

cgcaaguuca gcuucaucag ccagauccuc aagaacaagu ccgagaacag caaggagauc     3240

gccgagaagc ugaagcgggc cuaccaguuc aaguacaugc uggucucccg cgagaccgag     3300

accaaggacc aggagauguu caagaugacc guguuccccc gggagacgcc cggcaaccuc     3360

aucccgaaga agaaggggau ggcccccgag aucuacggcg gcuacaccaa gaacagcgac     3420

gcguacaugg ugaucgucaa gaucgacaag aagaaggaga ccgaguaccg cauccugggg     3480

auccccaccc gggagcuggu gaaccucaag aaggccgaga aggaggacca cuacgagucc     3540

uaccugaagg agauccuggc cccgcgcauc cucuacaaca agaacggcaa gcgggacaag     3600

aagaucacga gcuucgagau cgugaagucc aagauccccu acaagcaggu cauccaggac     3660

ggggacaaga aguucaugcu gggcagcucc accuacgugu acaacgccaa gcagcugacc     3720

cucagcaccg aguccaugaa ggccaucacg aacaacuucg acaaggacag cgacgagaac     3780

gacgcgcuga ucaaggccua cgacgagauc cuggacaagg uggacaagua ccucccccug     3840

uucgacauca acgccuuccg caagaagcug cacuccggcc gggagaaguu cgucgagcuc     3900

agccuggagg acaagaagga caucauccug aaggugcuca ucgggcugca cgacaacccc     3960

gccaccuccg accugaagac ccucggcuuc agcuccaccg agcuggggaa gaugcagaag     4020

aacagcggca ucgugcuguc cgggaacgcc aagcucaucu accagagccc gacgggccug     4080

uucaagaagu ccgucaagau cuccgaccug uga                                  4113


<210>  1969
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1969
augaccaaga ucaaggacga cuacaucgug ggccuggaca ucgggaccga cagcugcggc       60

ugggucgcca ugaacuccga caacgacauc cucaagcugc aggggaagac ggccaucggc      120

agccaccugu ucgaggcggg gaaguccgcc gccgaccgcc gccucuuccg gaccacccac      180

cggcgcauca agcggcgccg guggcgccug aagcugcucg aggaguucuu cgaccccuac      240

auggccaagg uggacccgua cuucuucgcc cggcugaagg agagcggccu gagcccccuc      300

gacaagcgca agaacguguu cuccaucguc uuccccaccg cggccgagga caagaaguuc      360

uacgacgacu accccacgau cuaccaccuc cgguacaagc ugaugaccga ggacgagaag      420

uucgaccucc gcgaggugua ccuggccauc caccacauca ucaaguaccg gggcaacuuc      480

cuguacaaca ccuccgugaa ggacuucaag gccagcaaga ucgacgucaa guccagcauc      540

gagaagcuca acgagcugua cgagaaccug gggcucgacc ugaacgugga guucaacauc      600

uccaacaccg cggagaucga gaaggugcug aaggacaagc agaucuucaa gcgcgacaag      660

gucaagaaga ucgccgagcu guucgccauc aagacggaca acaaggagca gagcaagcgg      720

aucaaggaca ucuccaagca ggucgccaac gccgugcugg gcuacaagac ccgcuucgac      780

accaucgcgc ugaaggaggu gagcaaggac gagcucuccg acuggaacuu caagcugagc      840

gacaucgacg ccgacuccaa guucgaggcc cugaugggga accucgacga gaacgagcag      900

gccauccugc ugaccaucaa ggagcuguuc aacgccguga cgcucaacgg caucguggag      960

gacgggaaca cccugagcga guccaugauc aacaaguaca acgaccaccg ggacgaccuc     1020

aagcugcuga aggaggucau cgagaaccac aucgaccgca agaaggcggu gaagcucgcc     1080

cuggccuacg accuguacgu gaacaaccgg cacggccagc ucauccaggc caagaagaag     1140

cugggcaaga ucaagccggg gagcaaggag gacuucuaca aggucgugaa caagaaccug     1200

gacgacagcc aggccuccaa ggagaucaag aagaagaucg agcucgacag cuucaugccc     1260

aagcagcgca ccaacgcgaa cggcgugauc cccuaccagc ugcaccagcu ggagcucgac     1320

aagaucaucg agaaccaguc caaguacuac cccuuccuga aggagaucaa cccggucagc     1380

ucccaccuga aggaggcccc cuacaagcuc gacgagcuga uccgguuccg cgugcccuac     1440

uacguggggc cccugaucag cccgaacgag uccaccaagg acauccagac gaagaagaac     1500

cagaacuucg ccuggaugau ccgcaaggag gagggccgga ucacccccug gaacuucgac     1560

cagaaggucg accgcaucga gagcgccaac aaguucauca agcggaugac caccaaggac     1620

acguaccugu ucggcgagaa cgugcucccc gccaacuccc ugcuguacca gaaguucacc     1680

guccucaacg agcugaacaa cauccgcauc aacgggaagc ggaucagcgu ggaccugaag     1740

caggagaucu acgagaaccu cuucaagaag cacaccaccg ugacggucaa gaagcuggag     1800

aacuaccuga agcagaacca caaccucgug aagguggaga ucaagggccu ggcggacgag     1860

aagaaguuca acuccgggcu gaccaccuac aaccgcuuca agaaccucaa caucuucgac     1920

aaccagaucg acgaccugaa guaccggaag gacuucgaga agaucaucga guggagcacc     1980

aucuucgagg acaaguccau cuacaaggag aagcugcgca gcaucgacug gcucaacgag     2040

aagcagauca acgcccuguc caacauccgg cugcagggcu gggggcgccu cagcaagaag     2100

cugcuggccc agcuccacga ccacaacggc cagacgauca ucgagcagcu gugggacucc     2160

cagaacaacu ucaugcagau cgucacccag gccgacuuca aggacgcgau cgccaaggcc     2220

aaccagaacc ugcucguggc caccagcgug gaggacaucc ugaacgacgc cuacaccucc     2280

cccgcgaaca agaaggccau ccggcagguc aucaaggugg uggacgacau cgucaaggcc     2340

gccagcggca aggugccgaa gcagaucgcc aucgaguuca cgcgcgacgc ggacgacaac     2400

cccaagcgga gccagacccg cggguccaag cugcagaagg uguacaagga ccucagcacc     2460

gagcuggccu ccaaggccau cgccgaggag cugaacgagg ccaucaagga caagaagcuc     2520

guccaggaca aguacuaccu guacuucaug cagcugggcc gggacgcgua caccggggag     2580

cccaucaaca ucgacgagau ccagaaguac gacaucgacc acauccuccc ccagagcuuc     2640

aucaaggacg acgcccugga caaccgcgug cuggugucca aggccgucaa caacgacaag     2700

agcgacaacg ugccggugaa gcuguucggc aacgagaugg ccgggaaccu cggcaucacg     2760

auccggaaga ugugggagga guggaagaac aucggccuga ucuccaagac caaguacaac     2820

aaccuccuga ccgaccccga ccacaucaac aaguacgaga gcgcgggguu cauccgccgg     2880

cagcuggucg agaccuccca gaucaucaag cucgugagca ccauccugca gucccgcuac     2940

cccaacacgg agaucaucac cgugaaggcc aaguacaacc acuaccugcg cgagaaguuc     3000

gaccucuaca agagccggga ggucaacgac uaccaccacg ccaucgacgc cuaccugucc     3060

gccaucugcg gcaaccugcu cuaccagaac uaccccaacc ugcgcccguu cuucguguac     3120

gggcaguaca agaaguucag cuccgacccc gacaaggaga aggagaucuu caacaagacc     3180

cggaaguuca gcuucaucuc ccagauccug aagaacaaga gcgagaacag caaggagauc     3240

gcggagaagc ucaagcgcgc cuaccaguuc aaguacaugc uggugucccg ggagaccgag     3300

acgaaggacc aggagauguu caagaugacc gucuuccccc gcgagacccc cggcaaccug     3360

aucccgaaga agaaggggau ggccaccgag aucuacggcg gcuacacgaa gaacagcgac     3420

gccuacaugg ugaucgugaa gaucgacaag aagaaggaga ccgaguaccg gauccucggg     3480

auccccaccc gcgagcuggu caaccugaag aaggccgaga aggaggacca cuacgagucc     3540

uaccucaagg agauccuggc gccccggauc cuguacaaca agaacggcaa gcgcgacaag     3600

aagaucacca gcuucgagau cgugaagucc aagaucccgu acaagcaggu cauccaggac     3660

ggggacaaga aguucaugcu cggcagcucc acguacgugu acaacgccaa gcagcugacc     3720

cugagcaccg aguccaugaa ggccaucacc aacaacuucg acaaggacag cgacgagaac     3780

gacgcccuca ucaaggccua cgacgagauc cuggacaagg uggacaagua ccugccccuc     3840

uucgacauca acgcguuccg gaagaagcug cacuccggcc gcgagaaguu cguggagcug     3900

agccucgagg acaagaagga caucauccug aagguccuga ucgggcucca cgacaacccc     3960

gccacguccg accugaagac ccugggcuuc agcuccaccg agcucgggaa gaugcagaag     4020

aacagcggca ucgugcuguc cgggaacgcc aagcugaucu accagagccc caccggccuc     4080

uucaagaagu ccgugaagau cagcgaccug uga                                  4113


<210>  1970
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1970
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgaugcagg aggagaagua cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccuca ucuaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgacc gcuucgacgu gcggaacacc      540

gacauccaga agcaguacca ggcguuccug gagaucuucg acaccacguu cgagaacaac      600

gaccugcucu cccagaacgu ggacgucgag gccauccuga ccgacaagau cagcaagucc      660

gccaagaagg accgcauccu ggcccgguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gcggacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucagcgccg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggucacc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggcguucu acaaguaccu gagcaagcuc     1140

cugacgaagc aggagggguc cgaguaccug cucgagaaga ucaagaacga ggacuuccug     1200

cgcaagcagc gcaccuucga caacggcagc aucccccacc aggugcaccu gaccgagcuc     1260

cgggccauca uccgccggca guccgaguac uacccguucc ugaaggagaa ccuggaccgc     1320

aucgagaaga uccucaccuu ccggaucccc uacuacgucg gcccccuggc ccgcgagaag     1380

uccgacuucg ccuggaugac gcggaagacc gacgacagca uccgccccug gaacuucgag     1440

gaccuggugg acaaggagaa guccgccgag gcguucaucc accggaugac caacaacgac     1500

cuguaccucc cggaggagaa ggugcugccc aagcacagcc ugaucuacga gaaguucacc     1560

gucuacaacg agcucacgaa ggugcgcuuc cuggccgagg gguucaagga cuuccaguuc     1620

cugaaccgga agcagaagga gaccaucuuc aacagccucu ucaaggagaa gcgcaaggug     1680

accgagaagg acaucaucuc cuuccugaac aaggucgacg gcuacgaggg gaucgccauc     1740

aagggcaucg agaagcaguu caacgccagc cuguccaccu accacgaccu caagaagauc     1800

cuggggaagg acuuccugga caacacggac aacgagcuca uccuggagga caucgugcag     1860

acccugaccc ucuucgagga ccgggagaug auccgcaagc ggcuggacau cuacaaggac     1920

uucuucaccg agagccagcu gaagaagcuc uaccgccggc acuacacggg cuggggccgc     1980

cuguccgcga agcugaucaa cgggauccgg aacaaggaga accagaagac cauccucgac     2040

uaccugaucg acgacggcag cgccaaccgc aacuucaugc agcugaucaa ggacgccggg     2100

cucuccuuca agcccaucau cgacaaggcc cgcaccggca gccacuccga caaccugaag     2160

gagguggucg gggagcuggc cggcagcccc gcgaucaaga agggcauccu ccagucccug     2220

aagaucgugg acgagcuggu gaaggucaug ggguacgagc cggagcagau cguggucgag     2280

auggcccggg agaaccagac cacggccaag ggccucagcc gcagccggca gcgccugacc     2340

acccugcggg agucccucgc caaccucaag agcaacaucc uggaggagaa gaagcccaag     2400

uacgugaagg accaggugga gaaccaccac cucuccgacg accgccuguu ccuguacuac     2460

cuccagaacg ggaaggacau guacaccgac gacgagcugg acaucgacaa ccugagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacuccau cgacaaccgg     2580

guccucguga gcuccgcgaa gaaccgcggc aagagcgacg acgugcccuc cgucgagauc     2640

gugaaggacu gcaagguguu cuggaagaag cugcuggacg ccaagcucau gagccagcgg     2700

aaguacgaca accugaccaa ggccgagcgc gggggccuga cguccgacga caaggcccgg     2760

uucauccagc gccagcucgu cgagacccgg cagaucacca agcacguggc ccgcauccug     2820

gacgagcggu ucaacaacga gcuggacagc aagggccgcc ggauccgcaa ggugaagauc     2880

gucacccuca aguccaaccu ggugagcaac uuccggaagg aguucggguu cuacaagauc     2940

cgcgagguga acaacuacca ccacgcgcac gacgccuacc ugaacgccgu cguggccaag     3000

gcgauccuca cgaaguaccc gcagcuggag cccgaguucg uguacggcga cuaccccaag     3060

uacaacuccu acaagacccg caagagcgcc accgagaagc uguucuucua cuccaacauc     3120

augaacuucu ucaagaccaa ggucacgcuc gccgacggga ccgugguggu caaggacgac     3180

aucgagguga acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

guccggaagg ugcugagcua cccccaggug aacaucguca agaagacgga gauccagacc     3300

ggcggguuca gcaaggaguc cauccuggcc cacggcaaca gcgacaagcu caucccgcgc     3360

aagaccaagg acaucuaccu ggaccccaag aaguacgggg gcuucgacuc ccccaaggug     3420

gcguacagcg ugcuggucgu ggccgacauc aagaagggga aggcccagaa gcucaagacc     3480

gugacggagc ugcugggcau caccaucaug gagcgguccc gcuucgagaa gaaccccagc     3540

gccuuccucg aguccaaggg cuaccugaac auccgggacg acaagcugau gauccucccg     3600

aaguacagcc uguucgagcu ggagaacggg cgccggcgcc uccuggccuc cgcgggcgag     3660

cugcagaagg ggaacgagcu cgcccugccc acccaguuca ugaaguuccu guaccucgcc     3720

agccgguaca acgagcugaa gggcaagccc gaggagaucg agcagaagca ggaguucguc     3780

gugcagcacg uguccuacuu cgacgacauc cugcagauca ucaacgacuu cagcaaccgc     3840

gucauccucg ccgacgccaa ccuggagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucccgg uggacgagcu cgcgaacaac aucaucaacc uguucaccuu cacgucccug     3960

ggggcccccg ccgccuucaa guucuucgac aagaucgugg accggaagcg cuacaccagc     4020

accaaggagg uccucaacuc cacccugauc cacaagagca ucacgggccu guacgagacc     4080

cggaucgacc ucggcaagcu gggggaggac uga                                  4113


<210>  1971
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1971
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgcuccagg aggagaagga cuaccacgag aaguucucca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cuccgccuga ucuacaucgc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgaca gcuucgacgu gcgcaacacc      540

gacaucucca agcaguacca ggacuuccug gagaucuuca acaccacguu cgagaacaac      600

gaccugcuca gccagaacgu ggacgucgag gcgauccuga ccgacaagau cuccaagagc      660

gccaagaagg accggauccu ggcccaguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gccgacuuca agaaguacuu caaccuggag      780

gacaagacgc cccuccaguu cgcgaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucuccgccg ccaagaagcu guacgacagc      900

gugcuccugu ccggcauccu gacggucauc gaccucagca ccaaggcccc gcuguccgcg      960

agcaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggccuucu acaaguaccu guccaagcuc     1140

cugaccaagc aggaggacag cgagaacuuc cuggagaaga ucaagaacga ggacuuccuc     1200

cgcaagcagc ggacguucga caacgggucc aucccccacc aggugcaccu gaccgagcug     1260

aaggcgauca uccgccgcca gagcgaguac uaccccuucc ucaaggagaa ccaggaccgg     1320

aucgagaaga uccugaccuu ccgcaucccg uacuacaucg gcccccuggc ccgggagaag     1380

uccgacuucg ccuggaugac ccgcaagacg gacgacagca uccggcccug gaacuucgag     1440

gaccucgucg acaaggagaa guccgccgag gccuucaucc accgcaugac caacaacgac     1500

uucuaccugc ccgaggagaa ggugcugccg aagcacagcc ugaucuacga gaaguucacc     1560

guguacaacg agcucaccaa gguccgguac aagaacgagc agggcgagac guacuucuuc     1620

gacuccaaca ucaagcagga gaucuucgac gggguguuca aggagcaccg caaggugagc     1680

aagaagaagc ugcuggacuu ccucgcgaag gaguacgagg aguuccggau cgucgacgug     1740

aucggccugg acaaggagaa caaggccuuc aacgccuccc uggggaccua ccacgaccuc     1800

gagaagaucc uggacaagga cuuccuggac aaccccgaca acgagagcau ccucgaggac     1860

aucgugcaga cccugacccu guucgaggac cgcgagauga ucaagaagcg gcucgagaac     1920

uacaaggacc uguucacgga gucccagcug aagaagcucu accgccggca cuacaccggc     1980

ugggggcgcc ugagcgccaa gcugaucaac ggcauccggg acaaggaguc ccagaagacc     2040

auccucgacu accugaucga cgacggccgc agcaaccgga acuucaugca gcugaucaac     2100

gacgacgggc ucuccuucaa gagcaucauc agcaaggcgc aggccggcuc ccacagcgac     2160

aaccugaagg aggucguggg ggagcuggcc ggcucccccg ccaucaagaa ggggauccuc     2220

cagagccuga agaucgugga cgagcugguc aaggugaugg gcuacgagcc cgagcagauc     2280

gucguggaga uggcccgcga gaaccagacc acgaaccagg gccggcgcaa cucccgccag     2340

cgguacaagc uccuggacga cggggugaag aaccuggcga gcgaccucaa cggcaacauc     2400

cugaaggagu acccgaccga caaccaggcc cugcagaacg agcgccucuu ccuguacuac     2460

cugcagaacg ggcgggacau guacaccggc gaggcccucg acaucgacaa ccugucccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagcau cgacaaccgc     2580

guccuggugu ccagcgccaa gaaccggggg aaguccgacg acgugcccag ccucgagauc     2640

gucaaggacu gcaagguguu cuggaagaag cugcuggacg cgaagcucau gucccagcgc     2700

aaguacgaca accugaccaa ggccgagcgg ggcggccuga cgagcgacga caaggcccgc     2760

uucauccagc ggcagcucgu ggagacccgc cagaucacca agcacgucgc ccggauccug     2820

gacgagcgcu ucaacaacga gcuggacucc aaggggcggc gcauccggaa ggugaagauc     2880

gugacccuca agagcaaccu ggucuccaac uuccgcaagg aguucggcuu cuacaagauc     2940

cgggagguga acaacuacca ccacgcccac gacgcguacc ugaacgccgu ggucgccaag     3000

gccauccuca ccaaguaccc ccagcuggag ccggaguucg uguacgggga cuaccccaag     3060

uacaacagcu acaagacgcg caagagcgcg accgagaagc uguucuucua cuccaacauc     3120

augaacuucu ucaagaccaa ggugacccuc gccgacggca cggucguggu gaaggacgac     3180

aucgagguca acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

gugcggaagg uccugagcua cccccagaac aacaucguga agaagaccga gauccagacc     3300

gggggcuucu ccaaggagag cauccuggcc cacgggaacu ccgacaagcu caucccccgc     3360

aagaccaagg acaucuaccu ggacccgaag aaguacggcg gguucgacag ccccaucgug     3420

gccuacuccg uccugguggu ggcggacauc aagaagggca aggcccagaa gcucaagacc     3480

gucaccgagc ugcugggcau cacgaucaug gagcggagcc gcuucgagaa gaaccccucc     3540

gccuuccucg agagcaaggg guaccugaac auccgcgccg acaagcugau cauccucccc     3600

aaguacuccc uguucgagcu ggagaacggc cggcgccggc uccuggccag cgcgggggag     3660

cugcagaagg gcaacgagcu cgcccugccg acccaguuca ugaaguuccu guaccucgcc     3720

ucccgcuaca acgagagcaa ggggaagccc gaggagaucg agaagaagca ggaguucgug     3780

aaccagcacg uguccuacuu cgacgacauc cugcagcuga ucaacgacuu cagcaagcgg     3840

gucauccucg ccgacgccaa ccuggagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucuccg uggacgagcu cgcgaacaac aucaucaacc uguucaccuu caccagccug     3960

ggcgcccccg ccgccuucaa guucuucgac aagaucgugg accgcaagcg guacacgucc     4020

accaaggagg uccucaacag cacccugauc caccagagca ucaccggccu guacgagacg     4080

cgcaucgacc ucgggaagcu gggcgaggac uga                                  4113


<210>  1972
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1972
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgcuccagg aggagaagga cuaccacgag aaguucucca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cuccgccuga ucuacaucgc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgaca gcuucgacgu gcgcaacacc      540

gacaucucca agcaguacca ggacuuccug gagaucuuca acaccacguu cgagaacaac      600

gaccugcuca gccagaacgu ggacgucgag gcgauccuga ccgacaagau cuccaagagc      660

gccaagaagg accggauccu ggcccaguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gccgacuuca agaaguacuu caaccuggag      780

gacaagacgc cccuccaguu cgcgaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucuccgccg ccaagaagcu guacgacagc      900

gugcuccugu ccggcauccu gacggucauc gaccucagca ccaaggcccc gcuguccgcg      960

agcaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggccuucu acaaguaccu guccaagcuc     1140

cugaccaagc aggaggacag cgagaacuuc cuggagaaga ucaagaacga ggacuuccuc     1200

cgcaagcagc ggacguucga caacgggucc aucccccacc aggugcaccu gaccgagcug     1260

aaggcgauca uccgccgcca gagcgaguac uaccccuucc ucaaggagaa ccaggaccgg     1320

aucgagaaga uccugaccuu ccgcaucccg uacuacaucg gcccccuggc ccgggagaag     1380

uccgacuucg ccuggaugac ccgcaagacg gacgacagca uccggcccug gaacuucgag     1440

gaccucgucg acaaggagaa guccgccgag gccuucaucc accgcaugac caacaacgac     1500

uucuaccugc ccgaggagaa ggugcugccg aagcacagcc ugaucuacga gaaguucacc     1560

guguacaacg agcucaccaa gguccgguac aagaacgagc agggcgagac guacuucuuc     1620

gacuccaaca ucaagcagga gaucuucgac gggguguuca aggagcaccg caaggugagc     1680

aagaagaagc ugcuggacuu ccucgcgaag gaguacgagg aguuccggau cgucgacgug     1740

aucggccugg acaaggagaa caaggccuuc aacgccuccc uggggaccua ccacgaccuc     1800

gagaagaucc uggacaagga cuuccuggac aaccccgaca acgagagcau ccucgaggac     1860

aucgugcaga cccugacccu guucgaggac cgcgagauga ucaagaagcg gcucgagaac     1920

uacaaggacc uguucacgga gucccagcug aagaagcucu accgccggca cuacaccggc     1980

ugggggcgcc ugagcgccaa gcugaucaac ggcauccggg acaaggaguc ccagaagacc     2040

auccucgacu accugaucga cgacggccgc agcaaccgga acuucaugca gcugaucaac     2100

gacgacgggc ucuccuucaa gagcaucauc agcaaggcgc aggccggcuc ccacagcgac     2160

aaccugaagg aggucguggg ggagcuggcc ggcucccccg ccaucaagaa ggggauccuc     2220

cagagccuga agaucgugga cgagcugguc aaggugaugg gcuacgagcc cgagcagauc     2280

gucguggaga uggcccgcga gaaccagacc acgaaccagg gccggcgcaa cucccgccag     2340

cgguacaagc uccuggacga cggggugaag aaccuggcga gcgaccucaa cggcaacauc     2400

cugaaggagu acccgaccga caaccaggcc cugcagaacg agcgccucuu ccuguacuac     2460

cugcagaacg ggcgggacau guacaccggc gaggcccucg acaucgacaa ccugucccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagcau cgacaaccgc     2580

guccuggugu ccagcgccaa gaaccggggg aaguccgacg acgugcccag ccucgagauc     2640

gucaaggacu gcaagguguu cuggaagaag cugcuggacg cgaagcucau gucccagcgc     2700

aaguacgaca accugaccaa ggccgagcgg ggcggccuga cgagcgacga caaggcccgc     2760

uucauccagc ggcagcucgu ggagacccgc cagaucacca agcacgucgc ccggauccug     2820

gacgagcgcu ucaacaacga gcuggacucc aaggggcggc gcauccggaa ggugaagauc     2880

gugacccuca agagcaaccu ggucuccaac uuccgcaagg aguucggcuu cuacaagauc     2940

cgggagguga acaacuacca ccacgcccac gacgcguacc ugaacgccgu ggucgccaag     3000

gccauccuca ccaaguaccc ccagcuggag ccggaguucg uguacgggga cuaccccaag     3060

uacaacagcu acaagacgcg caagagcgcg accgagaagc uguucuucua cuccaacauc     3120

augaacuucu ucaagaccaa ggugacccuc gccgacggca cggucguggu gaaggacgac     3180

aucgagguca acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

gugcggaagg uccugagcua cccccagaac aacaucguga agaagaccga gauccagacc     3300

gggggcuucu ccaaggagag cauccuggcc cacgggaacu ccgacaagcu caucccccgc     3360

aagaccaagg acaucuaccu ggacccgaag aaguacggcg gguucgacag ccccaucgug     3420

gccuacuccg uccugguggu ggcggacauc aagaagggca aggcccagaa gcucaagacc     3480

gucaccgagc ugcugggcau cacgaucaug gagcggagcc gcuucgagaa gaaccccucc     3540

gccuuccucg agagcaaggg guaccugaac auccgcgacg acaagcugau gauccucccc     3600

aaguacuccc uguucgagcu ggagaacggc cggcgccggc uccuggccag cgccggggag     3660

cugcagaagg gcaacgagcu cgcgcugccg acccaguuca ugaaguuccu guaccucgcc     3720

ucccgcuaca acgagagcaa ggggaagccc gaggagaucg agaagaagca ggaguucgug     3780

aaccagcacg uguccuacuu cgacgacauc cugcagcuga ucaacgacuu cagcaagcgg     3840

gucauccucg ccgacgccaa ccuggagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucuccg uggacgagcu cgccaacaac aucaucaacc uguucaccuu caccagccug     3960

ggcgcgcccg ccgccuucaa guucuucgac aagaucgugg accgcaagcg guacacgucc     4020

accaaggagg uccucaacag cacccugauc caccagagca ucaccggccu guacgagacg     4080

cgcaucgacc ucgggaagcu gggcgaggac uga                                  4113


<210>  1973
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1973
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cuccucuucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgaugcagg aggagaagua cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccuca ucuaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgacc gcuucgacgu gcggaacacc      540

gacauccaga agcaguacca ggcguuccug gagaucuucg acaccacguu cgagaacaac      600

gaccugcucu cccagaacgu ggacgucgag gccauccuga ccgacaagau cagcaagucc      660

gccaagaagg accgcauccu ggcccgguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gcggacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucagcgccg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggucacc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggcguucu acaaguaccu gagcaagcuc     1140

cugacgaagc aggagggguc cgaguaccug cucgagaaga ucaagaacga ggacuuccug     1200

cgcaagcagc gcaccuucga caacggcagc aucccccacc aggugcaccu gaccgagcuc     1260

cgggccauca uccgccggca guccgaguac uacccgcugc ugaaggagaa ccucgaccgc     1320

aucgagaaga uccugaccuu ccggaucccc uacuacgucg gcccccuggc ccgcgagaag     1380

uccgacuucg ccuggaugac gcggaagacc gacgacagca uccgccccug gaacuucgag     1440

gaccuggugg acaaggagaa guccgccgag gcguucaucc accggaugac caacaacgac     1500

cucuaccugc cggaggagaa ggugcugccc aagcacagcc ucaucuacga gaaguucacc     1560

gucuacaacg agcugacgaa ggugcgcuuc cuggccgagg gguucaagga cuuccaguuc     1620

cucaaccgga agcagaagga gaccaucuuc aacagccugu ucaaggagaa gcgcaaggug     1680

accgagaagg acaucaucuc cuuccugaac aaggucgacg gcuacgaggg gaucgccauc     1740

aagggcaucg agaagcaguu caacgccagc cucuccaccu accacgaccu gaagaagauc     1800

cuggggaagg acuuccucga caacacggac aacgagcuga uccuggagga caucgugcag     1860

acccucaccc uguucgagga ccgggagaug auccgcaagc ggcuggacau cuacaaggac     1920

uucuucaccg agagccagcu caagaagcug uaccgccggc acuacacggg cuggggccgc     1980

cuguccgcga agcucaucaa cgggauccgg aacaaggaga accagaagac cauccuggac     2040

uaccugaucg acgacggcag cgccaaccgc aacuucaugc agcucaucaa ggacgccggg     2100

cuguccuuca agcccaucau cgacaaggcc cgcaccggca gccacuccga caaccugaag     2160

gagguggucg gggagcucgc cggcagcccc gcgaucaaga agggcauccu gcagucccug     2220

aagaucgugg acgagcucgu gaaggucaug ggguacgagc cggagcagau cguggucgag     2280

auggcccggg agaaccagac cacggccaag ggccugagcc gcagccggca gcgccugacc     2340

acccuccggg agucccuggc caaccucaag agcaacaucc uggaggagaa gaagcccaag     2400

uacgugaagg accaggugga gaaccaccac cuguccgacg accgccuguu ccucuacuac     2460

cugcagaacg ggaaggacau guacaccgac gacgagcugg acaucgacaa ccucagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacuccau cgacaaccgg     2580

guccugguga gcuccgcgaa gaaccgcggc aagagcgacg acgugcccuc cgucgagauc     2640

gugaaggacu gcaagguguu cuggaagaag cugcucgacg ccaagcugau gagccagcgg     2700

aaguacgaca accugaccaa ggccgagcgc gggggccuca cguccgacga caaggcccgg     2760

uucauccagc gccagcuggu cgagacccgg cagaucacca agcacguggc ccgcauccug     2820

gacgagcggu ucaacaacga gcucgacagc aagggccgcc ggauccgcaa ggugaagauc     2880

gucacccuga aguccaaccu ggugagcaac uuccggaagg aguucggguu cuacaagauc     2940

cgcgagguga acaacuacca ccacgcgcac gacgccuacc ucaacgccgu cguggccaag     3000

gcgauccuga cgaaguaccc gcagcuggag cccgaguucg uguacggcga cuaccccaag     3060

uacaacuccu acaagacccg caagagcgcc accgagaagc uguucuucua cuccaacauc     3120

augaacuucu ucaagaccaa ggucacgcug gccgacggga ccgugguggu caaggacgac     3180

aucgagguga acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

guccggaagg ugcugagcua cccccaggug aacaucguca agaagacgga gauccagacc     3300

ggcggguuca gcaaggaguc cauccucgcc cacggcaaca gcgacaagcu gaucccgcgc     3360

aagaccaagg acaucuaccu ggaccccaag aaguacgggg gcuucgacuc ccccaucgug     3420

gcguacagcg ugcucgucgu ggccgacauc aagaagggga aggcccagaa gcugaagacc     3480

gugacggagc ugcucggcau caccaucaug gagcgguccc gcuucgagaa gaaccccagc     3540

gccuuccugg aguccaaggg cuaccuggac auccgggacg acaagcucau gauccugccg     3600

aaguacagcc uguucgagcu cgagaacggg cgccggcgcc ugcuggccuc cgcgggcgag     3660

cuccagaagg ggaacgagcu ggcccugccc acccaguuca ugaaguuccu cuaccuggcc     3720

agccgguaca acgagcugaa gggcaagccc gaggagaucg agcagaagca ggaguucguc     3780

gugcagcacg uguccuacuu cgacgacauc cuccagauca ucaacgacuu cagcaaccgc     3840

gucauccugg ccgacgccaa ccuggagaag aucaacaagc ucuaccagga caacaaggag     3900

aacaucccgg uggacgagcu ggcgaacaac aucaucaacc uguucaccuu cacgucccuc     3960

ggggcccccg ccgccuucaa guucuucgac aagaucgugg accggaagcg cuacaccagc     4020

accaaggagg uccugaacuc cacccugauc caccagagca ucacgggccu cuacgagacc     4080

cggaucgacc ugggcaagcu gggggaggac uga                                  4113


<210>  1974
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1974
augaagaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

gugaccgacg acuacaaggu gccggccaag aagaugcgcg ucuucgggga cacggaccgg      120

agccacauca agaagaaccu ccugggcacc cuccucuucg acgacgggaa caccgcggag      180

ucccgccggc ugaagcgcac cgcccggcgc cgguacacgc gccggcgcaa ccggauccug      240

uaccuccagg agaucuucac cgagagcaug aacgagaucg acgaguccuu cuuccaccgc      300

cuggacgaca gcuuccuggu gcccgaggac aagcggggcu ccaaguaccc caucuucgcc      360

acccuccagg aggagaagga guaccacaag caguucccca ccaucuacca ccuccgcaag      420

cagcuggccg acagcaagga gaaguccgac gugcggcuca ucuaccuggc ccuggcgcac      480

augaucaagu accgcggcca cuuccucuac gaggagacgu ucgacaucaa gaacaacgac      540

auccagaaga ucuucaacga guucaucaac aucuacgaca acaccuucga ggggagcucc      600

cugagcggcc agaacgccca ggucgaggcc aucuucaccg acaagaucuc caagagcgcc      660

aagcgggagc gcgugcugaa gcuguucccg gacgagaagu ccaccgggcu guucagcgag      720

uuccugaagc ucaucguggg caaccaggcg gacuucaaga agcacuucga ccuggaggag      780

aaggccccgc ugcaguucuc caaggacacg uacgacgagg agcucgagaa ccugcugggg      840

cagaucggcg acgacuucgc cgaccucuuc cugaucgcca agaagcugua cgacgccauc      900

cuccugagcg gcauccugac cgucaccgac cccuccacca aggcgccccu cagcgccagc      960

augaucgagc gguacgagaa ccaccagaag gaccuggccg cccugaagca guucaucaag     1020

aacaaccucc cggagaagua cguggaggug uucuccgacc agagcaagga cggguacgcc     1080

ggcuacaucg acgggaagac gacccaggag gcguucuaca aguacaucaa gaaccugcug     1140

uccaaguucg agggcgccga cuacuuccuc gacaagaucg agcgcgagga cuuccugcgg     1200

aagcagcgca ccuucgacaa cggcagcauc ccccaccaga uccaccucca ggagaugaac     1260

gccauccugc gccggcaggg ggagcacuac cccuuccuga aggagaaccg cgagaagauc     1320

gagaagaucc ugaccuuccg gauccccuac uacgucggcc cgcucgcccg cgacaaccgg     1380

gacuucuccu ggcugacgcg caacagcgac gagcccaucc ggcccuggaa cuucgaggag     1440

gugguggaca aggcccgcuc cgcggaggac uucauccacc ggaugaccaa cuacgaccug     1500

uaccuccccg aggagaaggu ccugccgaag cacagccugc uguacgagac cuucgccgug     1560

uacaacgagc ucaccaaggu gaaguucauc gccgaggggc ugcgcgacua ccaguuccug     1620

gacuccggcc agaagaagca gaucgucaac cagcuguuca aggagaagcg gaaggugacg     1680

gagaaggaca ucauccacua ccugcacaac guggacgggu acgacggcau cgagcugaag     1740

ggcaucgaga agcaguucaa cgccaaccuc agcaccuacc acgaccugcu gaagaucacc     1800

aaggacaagg aguucaugga cgaccccaag aacgaggaga uccucgagaa caucguccac     1860

acccugacga ucuucgagga ccgcgagaug aucaagcagc ggcuggccca guacgacacc     1920

cucuucgacg agaaggugau caaggcgcug acccgccggc acuacaccgg guggggcaag     1980

cuguccgcca agcucaucaa cgggauccgc gacaagcaga gcggcaagac gauccuggac     2040

uaccugaucg acgacgacaa gaucaaccgg aacuucaugc agcucaucaa cgacgacggg     2100

cuguccuuca aggagaucau ccagaaggcc cagguggucg gcaagaccga cgacgugaag     2160

caggucgugc aggagcugcc cggcagcccc gccaucaaga aggggauccu ccaguccauc     2220

aagaucgugg acgagcuggu caaggugaug ggcuacgcgc uggagagcau cgugaucgag     2280

auggcccgcg agaaccagac caccgcccgg gggaagaaga acucccagca gcgcuacaag     2340

cgcaucgagg acgcccucaa gaaccuggcc ccgggccugg acagcaacau ccucaaggag     2400

aaccccacgg acaacaucca gcugaagaac gaccggcugu uccucuacua ccugcagaac     2460

gggaaggaca uguacaccgg caagccccug gacaucaacc agcucuccag cuacgacauc     2520

gaccacauca ucccccaggc guucaucaag gacgacagca ucgacaaccg cguccugacc     2580

uccagcaagg acaaccgggg caaguccgac aacgugccga gccuggaggu gguccagaag     2640

cgcaaggccu ucuggcagca gcuccuggac uccaagcuga ucagcgagcg gaaguucaac     2700

aaccucacca aggccgagcg cgggggccug gacgagcggg acaagguggg guucauccgc     2760

cggcagcugg uggagaccca gcagaucacg aagaacgucg cccagauccu cgacgcccgc     2820

uucaacaccg aggugaagga gaagaaccag aagauccgga ccgugaagau caucacccug     2880

aaguccaacc uggucagcaa cuuccgcaag gaguucggcc ucuacaaggu gcgggagauc     2940

aacaacuacc accacgcgca cgacgccuac cugaacgccg uggucgccaa ggccauccug     3000

aagaaguacc ccaagcucga gcccgaguuc guguacggcg acuaccagaa guacgaccug     3060

aagaaguaca ucucccgcuu caagcccagc aaggagaucg agaaggcgac ggagaaguac     3120

uucuucuacu ccaaccugcu caacuucuuc aaggaggagg ugcuguacgc cgacgggacc     3180

auccggaagc gcgagaacau cgaguacagc aaggacaccg gcgagaucgc cugggacaag     3240

gagaaggacu ucgccaccau caagaagguc cuguccuacc cccaggugaa caucgugaag     3300

aagcgggagg uccagaccgg gggcuucagc aaggagucca uccuccccaa ggggaacagc     3360

gacaagcuga ucccccgcaa gacgaaggac auccuguggg acaccaccaa guacggcggc     3420

uucgacuccc cggugaucgc guacagcauc cuccugaucg ccgacaucga gaaggggaag     3480

gccaagaagc ugaagacggu gaagacccuc gucggcauca ccaucaugga gaaggccgcc     3540

uucgaggaga accccaucac cuuccuggag aacaaggggu accacaacgu gcggaaggag     3600

aacauccugu gccuccccaa guacagccug uucgagcugg agaacggccg ccggcgccuc     3660

cuggcguccg ccaaggagcu gcagaagggg aacgagaucg ugcuccccgu cugccugacg     3720

acccugcucu accacagcaa gaaccugcac aagcuggacg agccggagca ccucgaguac     3780

auccagaagc accgcaacga guucaaggac cugcugaacc ucguguccga guucagccag     3840

aaguacaucc uggccgaggc caaccuggag aagaucaagg accucuacgc cgacaacgag     3900

caggcggaca ucgagauccu ggccaacucc uucaucaacc ugcucaccuu caccgcccug     3960

ggcgcccccg ccgcguucaa guucuucggc aaggacgugg accggaagcg cuacacgacc     4020

gucagcgaga uccugaacgc cacccucauc caccagucca ucaccgggcu guacgagacg     4080

cggaucgacc ugagcaagcu cggcgaggag uga                                  4113


<210>  1975
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1975
augaagaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gccggccaag aagaugaagg ugcucgggaa cacggacaag      120

aacuacauca agaagaaccu gcugggcgcg cuccuguucg acagcgggaa caccgccgag      180

gaccgccggc ugaagcgcac cgcccggcgc cgguacaccc gccggcgcaa ccggcuccug      240

uaccugcagg acaucuucuc cgacgagaug aacaaggucg acgagagcuu cuuccaccgc      300

cucgaggacu ccuuccuggu gagcgaggac aagcggggcg accaccaccc caucuucggc      360

aacguggucg acgaggugaa guaccacgag caguucccca cgaucuacca ccuccgcaag      420

uaccucgccg acacccccga gaaggccgac cugcggcugg uguaccucgc gcuggcccac      480

augaucaagu uccgcgggca cuuccugauc gacgacccga agcucgacgu caagaacacc      540

gacucccagg agcuguucag ccaguuccug accgccuacg acgugacgca ggaggacucc      600

caccucgccg acgugaagau caacaucagc gacaucauca ccgagaagau gaacaagucc      660

cggaaggucg aggcgcugcu guuccacuuc cccagccaga agaagaacuc cuucuucggc      720

aaccucguga gccugguguu cgggcugcuc cccaacuuca agaccaacuu caaccuggcc      780

gaggacgcca agcugcagcu cuccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgagua caccgacguc uucguggccg cccgcaagcu guacgacucc      900

auccugcuca gcggcauccu gacggugaac gaccugucca ccaaggcgau ccucagcgcc      960

agcauggucc ggcgcuacga ggagcacaac aaggaccugg ccaagcugaa ggccuucgug     1020

aaggccaacu ucacccacga cguguucaag gaggucuucu ccgacgcgag caagaacggc     1080

uacgccgggu acaucgaggg caagaccucc caggaggacu ucuacaagua cgugaagaac     1140

cucaucggga agguggacgg cagcgccgac uucaucgaga agaucgaccg ggaggacuuc     1200

cugcgcaagc agcggacguu cgacaacggc uccauccccc accaggucca ccuggccgag     1260

augaaggcca uccuccgccg ccagggggag uacuacccgu uccugaagga gcacgcggag     1320

aagauccagc agauccugac cuuccggauc cccuacuacg ugggcccccu cgaccgccac     1380

gggcacagcg acuucgccug ggccuccuuc aacagcgagg agaaggucac ccccuggaac     1440

uucgaggaga ucaucgacaa gaacaagucc gcccaggccu ucaucgagcg gaugaccaac     1500

caggaccugu accugccgaa cgagaaggug cugcccaagc acagcccccu cuacgagaag     1560

uacacggugu acaacgagcu gaccaagauc aaguacguca ccgagaccgg cacggcgaag     1620

uucuucgacg ccaaccugaa gcaggagauc uucgacgggc uguucaagaa ggagcgcaag     1680

gugaccaagc ggaagcugcu gaccuuccuc gagaagaacu ucgacgaguu ccgcaucgug     1740

gacauccagg gccuggacaa ggagaccgag acguucaacg ccuccuacgg caucuaccag     1800

gaccugcuca agaucaucaa ggacaaggcc uucauggacg acagcgagaa cgcggacauc     1860

cuggaggaca ucguccugac ccucacccug uucgaggacc gggagaugau caagcagcgc     1920

cuggccaagu acgccgaccu cuucgacaag aaggugaucg acaaccuggc ccggcgccgg     1980

uacaccgggu ggggccgccu guccgccaag cuccugaacg ggauccggga caagcgcagc     2040

cggaagacga ucauggacua ccugguggac gacggccgcu ccaaccggaa ccucaugcag     2100

cugaucaccg acgacaaccu gaccuucaag gacgagaucg cgaaggccca guacagcgac     2160

aacuccgacg accuccacca ggucgugcag gaccuggccg ggagccccgc caucaagaag     2220

ggcauccugc aguccgugaa gaucgucgac gagcucguga aggugauggg cuacgagccg     2280

gagcagaucg ucguggagau ggcccgcgag aaccagacca cggcgaaggg gcggcgcaac     2340

agccagcagc gcuacaagcg gcuggaggag gccaucaaga accuggaccg cgaccucaac     2400

uacaagaucc ugaaggagca ccccaccgac aaccaggccc ugcagaacga ccgccucuuc     2460

cuguacuacc ugcagaacgg ccgggacaug uacaccgggg agagccucga caucaaccgg     2520

cuguccgacu acgacaucga ccacaucauc ccccaggccu ucaucaagga caacagcauc     2580

gacaaccgcg ugcuggucuc cagcgccaag aaccggggca aguccgacga cgugcccagc     2640

gaggacgugg ucaaccgcau gcggccguuc uggaacaagc uccugaacug cgggcugauc     2700

ucccagcgca aguacagcaa ccucaccaag aaggagcuga cccccaacga caaggcgggc     2760

uucaucaagc ggcagcuggu ggagacgcgc cagaucacca agcacguggc ccggauccuc     2820

gacgagcacu ucaacaagga guucgacgag gacaagaagc gcauccggaa ggucaaggug     2880

gugacccuga aguccaaccu ggucagcaac uuccgcaagg aguucgagcu cuacaaggug     2940

cgggagauca acgacuacca ccacgcccac gacgccuacc ugaacgcggu ggucgccaag     3000

gcccugcuca ugaaguaccc caagcuggag cccgaguucg uguacggcga guacccgaag     3060

uacaacuccu accgcgagcg gaagaccgcc acgcagcgca uguucuucua cagcaacauc     3120

augaacaugu ucaaguccaa ggugaagcug gccgacgagc ggaucaucga gcgccccaug     3180

aucgagguca acgacgagac cggggagauc gcgugggaca agaccaagca caucgccacc     3240

gugaagaagg ugcucagcua cccccagauc aacaucguca agaaggugga ggagcagacg     3300

ggcgggcuga ccaacgagac caucuacccc cgcggcuccu ucgacaagcu gcucaaccgg     3360

aagaacaccc uggaggccag caaguacggc ggguucggcu ccccgacggu ggccuacugc     3420

guccuggugg uggccgacag caagaacgag aacgggaagg ucaagaagcu caagcugggc     3480

agcgagcugc ucgggaucac cauccuggag aagaacgacu ucgagaacaa cucccuggac     3540

uuccucagcc gcaagggcua ccagggcgug caggagaaga agaucaucaa gcugcccaag     3600

uacucccugu ucgaguucca ggacgggagc cggcgcaugc ucuccagcgu gauggcgaac     3660

gacaacaagc ggggcgaccu gcagaaggcc aacgagauga uccugcccgc cuaccucguc     3720

aagcugcugu accacgccaa gcacaucaac uccguggggg agccggccca caagaacuac     3780

aucaacgagc acugcagcga guuccaggac cuccugaacu acauccgcca guucgcggac     3840

aaguucgugg acgucgacaa gaaccugucc aagauccgga gccucuccaa gagcauggcc     3900

gaguacuccg ugagcgagau cugcuccagc uucgugaacc ugcugacccu caccgcccug     3960

ggcgcccccg cggacuucaa guucuucggg acgaccaucc cccgcaagcg guacggcagc     4020

ccccagucca uccuguccgc cacccucauc caccagagca ucaccggccu guacgagacg     4080

cgcaucgacc ugagcaagcu cggggaggac uga                                  4113


<210>  1976
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1976
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cuccucuucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgaugcagg aggagaagua cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccuca ucuaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgacc gcuucgacgu gcggaacacc      540

gacauccaga agcaguacca ggcguuccug gagaucuucg acaccacguu cgagaacaac      600

gaccugcucu cccagaacgu ggacgucgag gccauccuga ccgacaagau cagcaagucc      660

gccaagaagg accgcauccu ggcccgguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gcggacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucagcgccg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggucacc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggcguucu acaaguaccu gagcaagcuc     1140

cugacgaagc aggagggguc cgaguaccug cucgagaaga ucaagaacga ggacuuccug     1200

cgcaagcagc gcaccuucga caacggcagc aucccccacc aggugcaccu gaccgagcuc     1260

cgggccauca uccgccggca guccgaguac uacccguucc ugaaggagaa ccuggaccgc     1320

aucgagaaga uccucaccuu ccggaucccc uacuacgucg gcccccuggc ccgcgagaag     1380

uccgacuucg ccuggaugac gcggaagacc gacgacagca uccgccccug gaacuucgag     1440

gaccuggugg acaaggagaa guccgccgag gcguucaucc accggaugac caacaacgac     1500

cuguaccucc cggaggagaa ggugcugccc aagcacagcc ugaucuacga gaaguucacc     1560

gucuacaacg agcucacgaa ggugcgcuuc cuggccgagg gguucaagga cuuccaguuc     1620

cugaaccgga agcagaagga gaccaucuuc aacagccucu ucaaggagaa gcgcaaggug     1680

accgagaagg acaucaucuc cuuccugaac aaggucgacg gcuacgaggg gaucgccauc     1740

aagggcaucg agaagcaguu caacgccagc cuguccaccu accacgaccu caagaagauc     1800

cuggggaagg acuuccugga caacacggac aacgagcuca uccuggagga caucgugcag     1860

acccugaccc ucuucgagga ccgggagaug auccgcaagc ggcuggacau cuacaaggac     1920

uucuucaccg agagccagcu gaagaagcuc uaccgccggc acuacacggg cuggggccgc     1980

cuguccgcga agcugaucaa cgggauccgg aacaaggaga accagaagac cauccucgac     2040

uaccugaucg acgacggcag cgccaaccgc aacuucaugc agcugaucaa ggacgccggg     2100

cucuccuuca agcccaucau cgacaaggcc cgcaccggca gccacuccga caaccugaag     2160

gagguggucg gggagcuggc cggcagcccc gcgaucaaga agggcauccu ccagucccug     2220

aagaucgugg acgagcuggu gaaggucaug ggguacgagc cggagcagau cguggucgag     2280

auggcccggg agaaccagac cacggccaag ggccucagcc gccugcggca gcgccugacc     2340

acccuccggg agagccuggc caaccugaag uccaacaucc ucgaggagaa gaagcccaag     2400

uacgugaagg accaggugga gaaccaccac cugagcgacg accgccuguu ccucuacuac     2460

cugcagaacg ggaaggacau guacaccgac gacgagcugg acaucgacaa ccucucccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagcau cgacaaccgg     2580

guccuggugu ccagcgcgaa gaaccgcggc aaguccgacg acgugcccag cgucgagauc     2640

gugaaggacu gcaagguguu cuggaagaag cugcucgacg ccaagcugau gucccagcgg     2700

aaguacgaca accugaccaa ggccgagcgc gggggccuca cgagcgacga caaggcccgg     2760

uucauccagc gccagcuggu cgagacccgg cagaucacca agcacguggc ccgcauccug     2820

gacgagcggu ucaacaacga gcucgacucc aagggccgcc ggauccgcaa ggugaagauc     2880

gucacccuca agagcaaccu gguguccaac uuccggaagg aguucggguu cuacaagauc     2940

cgcgagguga acaacuacca ccacgcgcac gacgccuacc ugaacgccgu cguggccaag     3000

gcgauccuga cgaaguaccc gcagcuggag cccgaguucg uguacggcga cuaccccaag     3060

uacaacagcu acaagacccg gaaguccgcc accgagaagc uguucuucua cagcaacauc     3120

augaacuucu ucaagaccaa ggucacgcug gccgacggga ccgugguggu caaggacgac     3180

aucgagguga acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

guccgcaagg ugcuguccua cccccaggug aacaucguca agaagacgga gauccagacc     3300

ggcggguuca gcaaggagag cauccucgcc cacggcaacu ccgacaagcu gaucccgcgc     3360

aagaccaagg acaucuaccu ggaccccaag aaguacgggg gcuucgacag ccccaucgug     3420

gcguacuccg ugcucgucgu ggccgacauc aagaagggga aggcccagaa gcugaagacc     3480

gugacggagc ugcucggcau caccaucaug gagcggagcc gcuucgagaa gaaccccucc     3540

gccuuccugg agagcaaggg cuaccugaac auccgggacg acaagcucau gauccugccg     3600

aaguacuccc uguucgagcu cgagaacggg cgccggcgcc ugcuggccag cgcggacgag     3660

cuccagaagg gcaacgagcu ggcccugccc acccaguuca ugaaguuccu cuaccuggcc     3720

ucccgguaca acgagcugaa ggggaagccc gaggagaucg agcagaagca ggaguucguc     3780

gugcagcacg ugagcuacuu cgacgacauc cuccagauca ucaacgacuu cuccaaccgc     3840

gucauccugg ccgacgccaa ccuggagaag aucaacaagc ucuaccagga caacaaggag     3900

aacaucccgg uggacgagcu ggcgaacaac aucaucaacc uguucaccuu cacgagccuc     3960

ggcgcccccg ccgccuucaa guucuucgac aagaucgugg accggaagcg cuacaccucc     4020

accaaggagg uccugaacag cacccugauc caccagucca ucacggggcu cuacgagacc     4080

cggaucgacc ugggcaagcu gggcgaggac uga                                  4113


<210>  1977
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1977
auggagaaga acaaggagua cgugaucggc cuggacaucg ggaccggcag cgucgggugg       60

uccgccauca gcaccgacaa cuaccagcuc auccgccaga agggcaagaa ccugaucggg      120

gugcggcugu ucaacuccgc cgagacggcg gaggagcgcc ggggcuaccg caccacccgg      180

cgccggcuca gccgccggcg cuggcggaug ucccugcuga acgacaucuu ccgccccgag      240

cucaacgaga ucgacgagaa cuuccuggcc cggcugaagu acagcugggu guccccgcuc      300

gaccagaaga acccccagcu gaacgagcag ggcaccaacg ccagccugua cggggacauc      360

gaggccgacc aggccuucca ccgcgcguac cccacgaucu accaccuccg gaagacccug      420

auggaggaca accagaagca cgaccugcgc gaggucuacc ucgccaucca ccacaucgug      480

aaguaccggg gccacuuccu gacccccgac uucgacgcca acucccagac caucgacauc      540

aaggggcugu ucaagcagcu cggcgacgug agcgggucca ucgagcugca ggccgaccag      600

acgagcaccg ucaucgcggc cuuccagucc auccugcucg acuucaccaa gaccccgacg      660

gagagcggcc gccuggccgg ccagcuguuc uccgaccaga acaaggacaa gcaggccaag      720

aagcaggucg aggagcuguu caagcuccug cgcgggggca aggggagccu cgccgacaug      780

uuccugcuga ccgaccucga caagguggug aagaaccagc ugaaguucgc guucgacgac      840

gccguggacg agaaccugaa ccucaucucc aaccugauca acgaggagca guacgagcug      900

cuccugaaca uccagaagcu gcggggcacc aucauccuca agcagauccu gggcgacgac      960

gagaccguga gcgacgccaa gguccggucc uacgaggagc acgcccacca gcugaaggag     1020

cucaaggccc aggugcugac gaaggacaac aaggagaagu ucgaggaggc guaccagcug     1080

auccagaagg gggacgccag ccgcaucgac ggccucaaga agcugaagac caucaucaac     1140

caggagcacg aggcccugcg ccucgacaag uacgaggccg acaagcugcu gaagcugauc     1200

gagcucgacc agcugcugcc cgugcagcgg agcaagcgca acggggagau cccccaccag     1260

cuccacgagu ucgagcugaa gaagaucauc gagaagcagg gccgguacua ccccuuccug     1320

cgcgagaccu ucgagcggga cgggcaggag uucaacaaga ucggccagcu cgucaacuuc     1380

cgcaucccgu acuacguggg cccccuggug gacccccggc uggccgggau ccaguccggc     1440

gaccagacca accacuggau gacgcagaag aaggcgggga agaucacccc cuggaacuac     1500

accgagcuca ucgacaucga caagagcgcc gccgaguuca ucaagcgcau gaccggcacg     1560

gacaccuacc uggucgggga gccgucccug cacaaggcca gccucaucua ccaggacuac     1620

aacgugcugc aggagcugaa caaccuccgg uacgacggca agccccugcc cguggacgcc     1680

aagcaggaga ucuacuccaa ccugcagguc agccucaaga acgcgaccaa gaacaagauc     1740

aacuccuucc ugaucagcga gggcuaccug caggacgggg aggagcucac cggcuucgcc     1800

gggaaggagc ugaagaacaa gcuguccagc uuccacuacu ucaagaacau cuucggcccc     1860

gagcgcauga acacgcucgg gaccgacaac cuggagaaga ucaccgagcu gcagaccgug     1920

uucgaggacc ggcacaugcu caagcgccag cugcagaagc ugggcuuccu cgacgacgac     1980

gagugcgaga agcuggccgg caagcacuuc acgggguggg gcaaccuguc caagaagcuc     2040

cugaacaccc cggccaucau cgaccccgag aucaacgaga acaagaucag ccugaucgac     2100

gcgcucuaca aggggcagcg gaaccugucc uucuacguga acaacaugga cgacaaguac     2160

caguuccgcg acuggaucga gagcgagaac cagucccagc aggucgagau cagccuggac     2220

gagaagauca agaaccucgc cggcuccccc gccaucaagc ggggcaucac ccagagcaug     2280

gccgugaucc aggacaucau ccgcgccaac aacgggaucu cccccaagcg gaucuaccug     2340

gaguucgcgc gcgagagcca gaacagcuac cagccgaacu cccggaucaa gcagaucgag     2400

aagaucuacg accagaacaa gaaggagcug augaaggucu accccgacgu guucaaggac     2460

gugcagggcg uccucaacaa cguggacgag aacaacagcc uguccgagaa ggccuuccug     2520

uacuucaugc agcucgggaa ggacauguac accggcgagg cccuggacuu cgacaagcug     2580

uacacguacg acaucgacca caucaucccc caggccauga ccaaggacaa cagcugggac     2640

aacaaggugc ucgucaccgc caucaagaac cgcgagaagu ccaaccaggu gcuggacaac     2700

gcgauccagg ccaagaugca caacuucugg cugcagcuca agaaggugaa ccugcugagc     2760

cagcgcaagu acgacacccu caugaagacc aacuucucca agggggacga gaagaaguuc     2820

aucgcccgga gccuggucga gacgcgccag aucaucaaga acguggccga gcugaucaag     2880

uccgaguucg accacgugga ggccaucccc auccggcagg agcucaccag cgacaugcgc     2940

cagcugaugg acuacaagaa gaaccggcug gucaacgacu accaccacgc gcacgacgcc     3000

cuccugaucg ccaccguggg cgaguucaug cagaagcgcg gcuucaugca ggggaugaac     3060

ccgucccaga acguguacca cggcuacacc caguacguca agaaguggcu gagcauggcc     3120

aagaaggcgc gguccgccga gaagcccgag cgcaucuucc cccuccaguu cguggugggg     3180

agcaugcgga uggccguccg cgacggccag guggacaagg ugacgcaccc cgccaccggg     3240

gagaucugcu ggaccgccga caacgaggcg uaccugcgga agguccuggg cuacaagaac     3300

auccucguga cccgcaaggu gaagaacgcc ggcaagcugu accagcgggu cacggugaag     3360

ccgaaggggu ccggcgaggc ccccgugaac caccagaagu ccgacaucaa caucuacggg     3420

ggcgucaccu ccgagcaggc cgccgagcug gugcucaucc gcaucaaggc gaagaacaac     3480

gccaagaaca uggugaucaa gauccccacc gccaucgcca accaggucgc caacaagacc     3540

acgaccuacg acgcguacgu gcggagccug aacaucccga acuacgccgg gaucauccug     3600

gaccagaucc cccucggcca gcugguguuc gacgagcgca ccagcuucua ccuggccucc     3660

gccgagauca agcacaacgc gcggcagcuc ugggucaacc agcacaucaa caacgugcug     3720

accaagaaga acaucaacga cgaggagcgc gacaacaucc ugaaccuccu gacgaacgag     3780

aagaaccugg agcgguucaa gcuccuggag cccgaccuga agcgcgugaa ggccgccaag     3840

cagcaguggg ccagcugcga cgaccaggag cagaugcaga uccuccggau caucaucaac     3900

aagaugcacg acgacgccag cccccagauc ccgggcaaga ucgaccccuu caagaacuac     3960

cuggacaagc aggggaaggg cuggcgcgac cugcgcacca agaaggggau gaagcucagc     4020

gacaacgcgg aguucgucua ccaguccccc accggccugu accaccggaa gaucagcauc     4080

aacgagcugc ucaagcagaa gcagcuggag uga                                  4113


<210>  1978
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1978
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgaugcagg aggagaagua cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccuca ucuaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgacc gcuucgacgu gcggaacacc      540

gacauccaga agcaguacca ggcguuccug gagaucuucg acaccacguu cgagaacaac      600

gaccugcucu cccagaacgu ggacgucgag gccauccuga ccgacaagau cagcaagucc      660

gccaagaagg accgcauccu ggcccgguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gcggacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucagcgccg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggucacc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggcguucu acaaguaccu gagcaagcuc     1140

cugacgaagc aggagggguc cgaguaccug cucgagaaga ucaagaacga ggacuuccug     1200

cgcaagcagc gcaccuucga caacggcagc aucccccacc aggugcaccu gaccgagcuc     1260

cgggccauca uccgccggca guccgaguac uacccguucc ugaaggagaa ccuggaccgc     1320

aucgagaaga uccucaccuu ccggaucccc uacuacgucg gcccccuggc ccgcgagaag     1380

uccgacuucg ccuggaugac gcggaagacc gacgacagca uccgccccug gaacuucgag     1440

gaccuggugg acaaggagaa guccgccgag gcguucaucc accggaugac caacaacgac     1500

cuguaccucc cggaggagaa ggugcugccc aagcacagcc ugaucuacga gaaguucacc     1560

gucuacaacg agcucacgaa ggugcgcuuc cuggccgagg gguucaagga cuuccaguuc     1620

cugaaccgga agcagaagga gaccaucuuc aacagccucu ucaaggagaa gcgcaaggug     1680

accgagaagg acaucaucuc cuuccugaac aaggucgacg gcuacgaggg gaucgccauc     1740

aagggcaucg agaagcaguu caacgccagc cuguccaccu accacgaccu caagaagauc     1800

cuggggaagg acuuccugga caacacggac aacgagcuca uccuggagga caucgugcag     1860

acccugaccc ucuucgagga ccgggagaug auccgcaagc ggcuggacau cuacaaggac     1920

uucuucaccg agagccagcu gaagaagcuc uaccgccggc acuacacggg cuggggccgc     1980

cuguccgcga agcugaucaa cgggauccgg aacaaggaga accagaagac cauccucgac     2040

uaccugaucg acgacggcag cgccaaccgc aacuucaugc agcugaucaa ggacgccggg     2100

cucuccuuca agcccaucau cgacaaggcc cgcaccggca gccaccugga caaccugaag     2160

gagguggucg gggagcucgc cggcuccccc gcgaucaaga agggcauccu gcagagccug     2220

aagaucgugg acgagcucgu gaaggucaug ggguacgagc cggagcagau cguggucgag     2280

auggcccggg agaaccagac cacggccaag ggccuguccc gcagccggca gcgccugacc     2340

acccuccggg agagccuggc caaccugaag uccaacaucc ucgaggagaa gaagcccaag     2400

uacgugaagg accaggugga gaaccaccac cugagcgacg accgccuguu ccucuacuac     2460

cugcagaacg ggaaggacau guacaccgac gacgagcugg acaucgacaa ccucucccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagcau cgacaaccgg     2580

guccuggugu ccagcgcgaa gaaccgcggc aaguccgacg acgugcccag ccuggagauc     2640

gucaaggacu gcaagguguu cuggaagaag cuccuggacg ccaagcugau gucccagcgg     2700

aaguacgaca accucaccaa ggccgagcgc gggggccuga cgagcgacga caaggcccgg     2760

uucauccagc gccagcuggu ggagacccgg cagaucacca agcacgucgc ccgcauccuc     2820

gacgagcggu ucaacaacga gcuggacucc aagggccgcc ggauccgcaa ggugaagauc     2880

gugacccuca agagcaaccu cgucuccaac uuccggaagg aguucggguu cuacaagauc     2940

cgcgagguga acaacuacca ccacgcgcac gacgccuacc ugaacgccgu ggucgccaag     3000

gcgauccuga cgaaguaccc gcagcucgag cccgaguucg uguacggcga cuaccccaag     3060

uacaacagcu acaagacccg gaaguccgcc accgagaagc uguucuucua cagcaacauc     3120

augaacuucu ucaagaccaa ggugacgcug gccgacggga ccgucguggu gaaggacgac     3180

aucgagguca acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

gugcgcaagg uccucuccua cccccaggug aacaucguga agaagacgga gguccagacc     3300

ggcggguuca gcaaggagag cauccuggcc cacggcaacu ccgacaagcu gaucccgcgc     3360

aagaccaagg acaucuaccu cgaccccaag aaguacgggg gcuucgacag ccccaucgug     3420

gcguacuccg ugcuggucgu ggccgacauc aagaagggga aggcccagaa gcugaagacc     3480

gugacggagc uccugggcau caccaucaug gagcggagcc gcuucgagaa gaaccccucc     3540

gccuuccugg agagcaaggg cuaccucaac auccggaccg acaagcugau cauccugccg     3600

aaguacuccc ucuucgagcu ggagaacggg cgccggcgcc ugcucgccag cgcgggcgag     3660

cugcagaagg ggaacgagcu ggcccucccc acccaguaca ugaaguuccu guaccuggcc     3720

ucccgguaca acgagcucaa gggcaagccc gaggagaucg agcagaagca ggaguucguc     3780

gugcagcacg ugagcuacuu cgacgacauc cugcagauca ucaacgacuu cuccaaccgc     3840

gucauccugg ccgacgccaa ccucgagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucccgg uggacgagcu ggcgaacaac aucaucaacc ucuucacguu caccagccug     3960

ggggcccccg ccgccuucaa guucuucgac aagaucgugg accggaagcg cuacaccucc     4020

accaaggagg uccugaacag cacgcucauc caccagucca ucaccggccu guacgagacc     4080

cggaucgacc ugggcaagcu cggggagggc uga                                  4113


<210>  1979
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1979
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgcuccagg aggagaagga cuaccacgag aaguucucca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cuccgccuga ucuacaucgc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgaca gcuucgacgu gcgcaacacc      540

gacaucucca agcaguacca ggacuuccug gagaucuuca acaccacguu cgagaacaac      600

gaccugcuca gccagaacgu ggacgucgag gcgauccuga ccgacaagau cuccaagagc      660

gccaagaagg accggauccu ggcccaguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gccgacuuca agaaguacuu caaccuggag      780

gacaagacgc cccuccaguu cgcgaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucuccgccg ccaagaagcu guacgacagc      900

gugcuccugu ccggcauccu gacggucauc gaccucagca ccaaggcccc gcuguccgcg      960

agcaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggccuucu acaaguaccu guccaagcuc     1140

cugaccaagc aggaggacag cgagaacuuc cuggagaaga ucaagaacga ggacuuccuc     1200

cgcaagcagc ggacguucga caacgggucc aucccccacc aggugcaccu gaccgagcug     1260

aaggcgauca uccgccgcca gagcgaguac uaccccuucc ucaaggagaa ccaggaccgg     1320

aucgagaaga uccugaccuu ccgcaucccg uacuacaucg gcccccuggc ccgggagaag     1380

uccgacuucg ccuggaugac ccgcaagacg gacgacagca uccggcccug gaacuucgag     1440

gaccucgucg acaaggagaa guccgccgag gccuucaucc accgcaugac caacaacgac     1500

uucuaccugc ccgaggagaa ggugcugccg aagcacagcc ugaucuacga gaaguucacc     1560

guguacaacg agcucaccaa gguccgguac aagaacgagc agggcgagac guacuucuuc     1620

gacuccaaca ucaagcagga gaucuucgac gggguguuca aggagcaccg caaggugagc     1680

aagaagaagc ugcuggacuu ccucgcgaag gaguacgagg aguuccggau cgucgacgug     1740

aucggccugg acaaggagaa caaggccuuc aacgccuccc uggggaccua ccacgaccuc     1800

gagaagaucc uggacaagga cuuccuggac aaccccgaca acgagagcau ccucgaggac     1860

aucgugcaga cccugacccu guucgaggac cgcgagauga ucaagaagcg gcucgagaac     1920

uacaaggacc uguucacgga gucccagcug aagaagcucu accgccggca cuacaccggc     1980

ugggggcgcc ugagcgccaa gcugaucaac ggcauccggg acaaggaguc ccagaagacc     2040

auccucgacu accugaucga cgacggccgc agcaaccgga acuucaugca gcugaucaac     2100

gacgacgggc ucuccuucaa gagcaucauc agcaaggcgc aggccggcuc ccacagcgac     2160

aaccugaagg aggucguggg ggagcuggcc ggcucccccg ccaucaagaa ggggauccuc     2220

cagagccuga agaucgugga cgagcugguc aaggugaugg gcuacgagcc cgagcagauc     2280

gucguggaga uggcccgcga gaaccagacc acgaaccagg gccggcgcaa cucccgccag     2340

cgguacaagc uccuggacga cggggugaag aaccuggcga gcgaccucaa cggcaacauc     2400

cugaaggagu acccgaccga caaccaggcc cugcagaacg agcgccucuu ccuguacuac     2460

cugcagaacg ggcgggacau guacaccggc gaggcccucg acaucgacga ccugucccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagcau cgacaaccgc     2580

guccuggugu ccagcgccaa gaaccggggg aaguccgacg acgugcccag ccucgagauc     2640

gucaaggacu gcaagguguu cuggaagaag cugcuggacg cgaagcucau gucccagcgc     2700

aaguacgaca accugaccaa ggccgagcgg ggcggccuga cgagcgacga caaggcccgc     2760

uucauccagc ggcagcucgu ggagacccgc cagaucacca agcacgucgc ccggauccug     2820

gacgagcgcu ucaacaacga gcuggacucc aaggggcggc gcauccggaa ggugaagauc     2880

gugacccuca agagcaaccu ggucuccaac uuccgcaagg aguucggcuu cuacaagauc     2940

cgggagguga acaacuacca ccacgcccac gacgcguacc ugaacgccgu ggucgccaag     3000

gccauccuca ccaaguaccc ccagcuggag ccggaguucg uguacgggga cuaccccaag     3060

uacaacagcu acaagacgcg caagagcgcg accgagaagc uguucuucua cuccaacauc     3120

augaacuucu ucaagaccaa ggugacccuc gccgacggca cggucguggu gaaggacgac     3180

aucgagguca acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

gugcggaagg uccugagcua cccccagaac aacaucguga agaagaccga gauccagacc     3300

gggggcuucu ccaaggagag cauccuggcc cacgggaacu ccgacaagcu caucccccgc     3360

aagaccaagg acaucuaccu ggacccgaag aaguacggcg gguucgacag ccccaucgug     3420

gccuacuccg uccugguggu ggcggacauc aagaagggca aggcccagaa gcucaagacc     3480

gucaccgagc ugcugggcau cacgaucaug gagcggagcc gcuucgagaa gaaccccucc     3540

gccuuccucg agagcaaggg guaccugaac auccgcgccg acaagcugau cauccucccc     3600

aaguacuccc uguucgagcu ggagaacggc cggcgccggc uccuggccag cgcgggggag     3660

cugcagaagg gcaacgagcu cgcccugccg acccaguuca ugaaguuccu guaccucgcc     3720

ucccgcuaca acgagagcaa ggggaagccc gaggagaucg agaagaagca ggaguucgug     3780

aaccagcacg uguccuacuu cgacgacauc cugcagcuga ucaacgacuu cagcaagcgg     3840

gucauccucg ccgacgccaa ccuggagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucuccg uggacgagcu cgcgaacaac aucaucaacc uguucaccuu caccagccug     3960

ggcgcccccg ccgccuucaa guucuucgac aagaucgugg accgcaagcg guacacgucc     4020

accaaggagg uccucaacag cacccugauc caccagagca ucaccggccu guacgagacg     4080

cgcaucgacc ucgggaagcu gggcgaggac uga                                  4113


<210>  1980
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1980
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgcuccagg aggagaagga cuaccacgag aaguucucca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gcgcgccgac cuccggcuga ucuacaucgc ccuggcccac      480

aucaucaagu uccgcgggca cuuccucauc gaggacgaca gcuucgacgu gcggaacacc      540

gacaucucca agcaguacca ggacuuccug gagaucuuca acaccacguu cgagaacaac      600

gaccugcuca gccagaacgu ggacgucgag gcgauccuga ccgacaagau cuccaagagc      660

gccaagaagg accgcauccu ggcccaguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gccgacuuca agaaguacuu caaccuggag      780

gacaagacgc cccuccaguu cgcgaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucuccgccg ccaagaagcu guacgacagc      900

gugcuccugu ccggcauccu gacggucauc gaccucagca ccaaggcccc gcuguccgcg      960

agcaugaucc agcgguacga cgagcaccgc gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggccuucu acaaguaccu guccaagcuc     1140

cugaccaagc aggaggacag cgagaacuuc cuggagaaga ucaagaacga ggacuuccuc     1200

cggaagcagc gcacguucga caacgggucc aucccccacc aggugcaccu gaccgagcug     1260

aaggcgauca uccgccggca gagcgaguac uaccccuucc ucaaggagaa ccaggaccgc     1320

aucgagaaga uccugaccuu ccggaucccg uacuacaucg gcccccuggc ccgcgagaag     1380

uccgacuucg ccuggaugac ccggaagacg gacgacagca uccgccccug gaacuucgag     1440

gaccucgucg acaaggagaa guccgccgag gccuucaucc accggaugac caacaacgac     1500

uucuaccugc ccgaggagaa ggugcugccg aagcacagcc ugaucuacga gaaguucacc     1560

guguacaacg agcucaccaa gguccgcuac aagaacgagc agggcgagac guacuucuuc     1620

gacuccaaca ucaagcagga gaucuucgac gggguguuca aggagcaccg gaaggugagc     1680

aagaagaagc ugcuggacuu ccucgcgaag gaguacgagg aguuccgcau cgucgacgug     1740

aucggccugg acaaggagaa caaggccuuc aacgccuccc uggggaccua ccacgaccuc     1800

gagaagaucc uggacaagga cuuccuggac aaccccgaca acgagagcau ccucgaggac     1860

aucgugcaga cccugacccu guucgaggac cgggagauga ucaagaagcg ccucgagaac     1920

uacaaggacc uguucacgga gucccagcug aagaagcucu accggcgcca cuacaccggc     1980

ugggggcggc ugagcgccaa gcugaucaac ggcauccgcg acaaggaguc ccagaagacc     2040

auccucgacu accugaucga cgacggccgg agcaaccgca acuucaugca gcugaucaac     2100

gacgacgggc ucuccuucaa gagcaucauc agcaaggcgc aggccggcuc ccacagcgac     2160

aaccugaagg aggucguggg ggagcuggcc ggcucccccg ccaucaagaa ggggauccuc     2220

cagagccuga agaucgugga cgagcugguc aaggugaugg gcuacgagcc cgagcagauc     2280

gucguggaga uggcccggga gaaccagacc acgaaccagg gccgccgcaa cucccggcag     2340

cgcuacaagc uccuggacga cggggugaag aaccuggcga gcgaccucaa cggcaacauc     2400

cugaaggagu acccgaccga caaccaggcc cugcagaacg agcgccucuu ccuguacuac     2460

cugcagaacg ggcgggacau guacaccggc gaggcccucg acaucgacga ccugucccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagcau cgacaaccgg     2580

guccuggugu ccagcgccaa gaaccgcggg aaguccgacg acgugcccag ccucgagauc     2640

gucaaggacu gcaagguguu cuggaagaag cugcuggacg cgaagcucau gucccagcgg     2700

aaguacgaca accugaccaa ggccgagcgc ggcggccuga cgagcgacga caaggcccgg     2760

uucauccagc gccagcucgu ggagacccgg cagaucacca agcacgucgc ccgcauccug     2820

gacgagcggu ucaacaacga gcuggacucc aaggggcgcc ggauccgcaa ggugaagauc     2880

gugacccuca agagcaaccu ggucuccaac uuccggaagg aguucggcuu cuacaagauc     2940

cgcgagguga acaacuacca ccacgcccac gacgcguacc ugaacgccgu ggucgccaag     3000

gccauccuca ccaaguaccc ccagcuggag ccggaguucg uguacgggga cuaccccaag     3060

uacaacagcu acaagacgcg caagagcgcg accgagaagc uguucuucua cuccaacauc     3120

augaacuucu ucaagaccaa ggugacccuc gccgacggca cggucguggu gaaggacgac     3180

aucgagguca acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

gugcggaagg uccugagcua cccccagaac aacaucguga agaagaccga gauccagacc     3300

gggggcuucu ccaaggagag cauccuggcc cacgggaacu ccgacaagcu caucccccgg     3360

aagaccaagg acaucuaccu ggacccgaag aaguacggcg gguucgacag ccccaucgug     3420

gccuacuccg uccugguggu ggcggacauc aagaagggca aggcccagaa gcucaagacc     3480

gucaccgagc ugcugggcau cacgaucaug gagcgcagcc gcuucgagaa gaaccccucc     3540

gccuuccucg agagcaaggg guaccugaac auccgggccg acaagcugau cauccucccc     3600

aaguacuccc uguucgagcu ggagaacggc cgccggcgcc uccuggccag cgcgggggag     3660

cugcagaagg gcaacgagcu cgcccugccg acccaguuca ugaaguuccu guaccucgcc     3720

ucccgguaca acgagagcaa ggggaagccc gaggagaucg agaagaagca ggaguucgug     3780

aaccagcacg uguccuacuu cgacgacauc cugcagcuga ucaacgacuu cagcaagcgc     3840

gucauccucg ccgacgccaa ccuggagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucuccg uggacgagcu cgcgaacaac aucaucaacc uguucaccuu caccagccug     3960

ggcgcccccg ccgccuucaa guucuucgac aagaucgugg accggaagcg cuacacgucc     4020

accaaggagg uccucaacag cacccugauc caccagagca ucaccggccu guacgagacg     4080

cggaucgacc ucgggaagcu gggcgaggac uga                                  4113


<210>  1981
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1981
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcgucc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug agcaaggugg acgacuccuu cuuccaccgg      300

cuggaggaca gcuuccuggu cgaggacgac aagcgcggcu ccaaguaccc caucuucgcc      360

acgaugcagg aggagaagga cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggccg acaagaagga gaaggcggac cugcgccucu ucuaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgaca gcuucgacgu gcgcaacacc      540

gacauccagc ggcaguacca ggccuuccug gagaucuucg acaccacguu cgagaacaac      600

caccuccucu cccagaacau cgacguggag ggcauccuga ccgacaagau cagcaagucc      660

gcgaagaagg accgcauccu ggcccaguac cccaaccaga aguccaccgg gaucuucgcc      720

gaguuccuca agcugaucgu cggcaaccag gccgacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

gggcagaucg gcgacgaguu cgcggaccug uucagcgugg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggucacc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcgguacga cgagcaccgc gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucaccg acagcuccaa ggacggguac     1080

gccggcuaca ucgaggggaa gacgaaccag ggcgccuucu acaaguaccu gagcaagcuc     1140

cugaccaagc aggagggcuc cgaguacuuc cuggagaaga ucaagaacga ggacuuccuc     1200

cggaagcagc gcaccuucga caacgggagc aucccccacc aggugcaccu gaccgagcug     1260

aaggccauca uccgccggca guccgaguac uacccguucc ucaaggagaa ccuggaccgc     1320

aucgagaaga uccugacguu ccggaucccc uacuacgucg gcccccucgc ccgcgagaag     1380

uccgacuucg ccuggaugac ccggaagacc gacgacagca uccgccccug gaacuucgag     1440

gagcuggugg acaaggaggc gagcgccgag gccuucaucc accggaugac caacaacgac     1500

cuguaccugc cggaggagaa ggugcucccc aagcacuccc ugaucuacga gaaguucacg     1560

gucuacaacg agcugaccaa ggugcgcuac aagaacgagc agggggagac cuacuucuuc     1620

gacagcaaca ucaagcagga gaucuucgac ggcguguuca aggagcaccg gaaggucucc     1680

aagaagaagc uccuggacuu ccuggccaag gaguacgagg aguuccgcau cguggacgug     1740

aucgggcucg acaaggagaa caaggccuuc aacgcgagcc ugggcaccua ccacgaccug     1800

aagaagaucc ucgacaagga cuuccuggac aaccccgaca acgaguccau ccuggaggac     1860

aucguccaga cgcucacccu guucgaggac cgggagauga ucaagaagcg ccuggagaac     1920

uacaaggacc ucuucaccga gagccagcug aagaagcugu accggcgcca cuacaccggc     1980

ugggggcggc ucuccgccaa gcugaucaac ggcauccgcg acaaggagag ccagaagacg     2040

auccuggacu accucaucga cgacgggaag uccaaccgga acuucaugca gcugauccac     2100

gacgacggcc ugagcuucaa guccaucauc agcaaggccc aggccgggag ccacuccgac     2160

aaccucaagg aggugguggg cgagcuggcg ggcagccccg ccaucaagaa ggggauccug     2220

cagucccuca agaucgucga cgagcuggug aaggucaugg gcuacgagcc ggagcagauc     2280

gugguggaga uggcccgcga gaaccagacc accaaccagg ggcggcgcaa cagccgccag     2340

cgguacaagc ugcucgacga cggcgucaag aaccuggccu ccgaccugaa cgggaacauc     2400

cucaaggagu accccaccga caaccaggcc cugcagaacg agcgccuguu ccucuacuac     2460

cugcagaacg gccgggacau guacacgggc aaggcgcugg acaucgacaa ccucagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacuccau cgacaaccgc     2580

gugcugguga gcuccgccaa gaaccggggg aaguccgacg acguccccag ccuggagauc     2640

gugaaggacu gcaagguguu cuggaagaag cuccuggacg ccaagcugau gagccagcgc     2700

aaguacgaca accucaccaa ggccgagcgg ggcgggcuga ccuccgacga caaggcgcgc     2760

uucauccagc ggcagcuggu cgagacccgc cagaucacca agcacguggc ccggauccuc     2820

gacgagcgcu ucaacaacga gcuggacagc aagggccggc gcauccggaa ggugaagauc     2880

gucacgcuga aguccaaccu cgugagcaac uuccgcaagg aguucggcuu cuacaagauc     2940

cgggagguga acaacuacca ccacgcccac gacgccuacc ugaacgccgu cguggcgaag     3000

gccauccuga ccaaguaccc gcagcucgag cccgaguucg uguacgggga cuaccccaag     3060

uacaacuccu acaagacccg caagagcgcc accgagaagc uguucuucua cagcaacauc     3120

augaacuucu ucaagacgaa ggucacccug gccgacggca ccgugguggu caaggacgac     3180

aucgagguga acaacgacac cggggagauc gugugggaca agaagaagca cuucgcgacg     3240

guccggaagg ugcucuccua cccccaggug aacaucguca agaagaccga gauccagacc     3300

ggcggguuca gcaaggaguc cauccuggcc cacggcaaca gcgacaagcu gaucccgcgc     3360

aagaccaagg acaucuaccu cgaccccaag aaguacggcg gguucgacuc ccccaucgug     3420

gccuacagcg ugcuggucgu ggccgacauc aagaagggca aggcccagaa gcugaagacg     3480

gugaccgagc uccuggggau caccaucaug gagcgguccc gcuucgagaa gaaccccagc     3540

gcguuccugg aguccaaggg cuaccucaac auccgcgacg acaagcugau gauccugccg     3600

aaguacagcc ucuucgagcu ggagaacggg cggcgccggc ugcucgccuc cgccggcgag     3660

cugcagaagg gcaacgagcu ggcccucccc acccaguuca ugaaguuccu guaccuggcc     3720

agccgcuaca acgagcucaa ggggaagccc gaggagaucg agcagaagca ggaguucguc     3780

gugcagcacg uguccuacuu cgacgacauc cugcagauca ucaacgacuu cagcaaccgg     3840

gucauccugg cggacgccaa ccucgagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucuccg uggacgagcu ggccaacaac aucaucaacc ucuucacguu caccagccug     3960

ggcgccccgg ccgcguucaa guucuucgac aagaucgugg accgcaagcg guacaccucc     4020

accaaggagg uccugaacag cacgcucauc caccagucca ucaccgggcu guacgagacc     4080

cgcaucgacc ugggcaagcu cggggaggac uga                                  4113


<210>  1982
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1982
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgaugcagg aggagaagua cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccuca ucuaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgacc gcuucgacgu gcggaacacc      540

gacauccaga agcaguacca ggcguuccug gagaucuucg acaccacguu cgagaacaac      600

gaccugcucu cccagaacgu ggacgucgag gccauccuga ccgacaagau cagcaagucc      660

gccaagaagg accgcauccu ggcccgguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gcggacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucagcgccg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggucacc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcacuacga cgagcaccgc gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggcguucu acaaguaccu gagcaagcuc     1140

cugacgaagc aggagggguc cgaguaccug cucgagaaga ucaagaacga ggacuuccug     1200

cggaagcagc gcaccuucga caacggcagc aucccccacc aggugcaccu gaccgagcuc     1260

cgcgccauca uccggcgcca guccgaguac uacccguucc ugaaggagaa ccuggaccgg     1320

aucgagaaga uccucaccuu ccgcaucccc uacuacgucg gcccccuggc ccgggagaag     1380

uccgacuucg ccuggaugac gcgcaagacc gacgacagca uccggcccug gaacuucgag     1440

gaccuggugg acaaggagaa guccgccgag gcguucaucc accgcaugac caacaacgac     1500

cuguaccucc cggaggagaa ggugcugccc aagcacagcc ugaucuacga gaaguucacc     1560

gucuacaacg agcucacgaa ggugcgguuc cuggccgagg gguucaagga cuuccaguuc     1620

cugaaccgca agcagaagga gaccaucuuc aacagccucu ucaaggagaa gcggaaggug     1680

accgagaagg acaucaucuc cuuccugaac aaggucgacg gcuacgaggg gaucgccauc     1740

aagggcaucg agaagcaguu caacgccagc cuguccaccu accacgaccu caagaagauc     1800

cuggggaagg acuuccugga caacacggac aacgagcuca uccuggagga caucgugcag     1860

acccugaccc ucuucgagga ccgcgagaug auccggaagc gccuggacau cuacaaggac     1920

uucuucaccg agagccagcu gaagaagcuc uaccggcgcc acuacacggg cuggggccgg     1980

cuguccgcga agcugaucaa cgggauccgc aacaaggaga accagaagac cauccucgac     2040

uaccugaucg acgacggcag cgccaaccgg aacuucaugc agcugaucaa ggacgccggg     2100

cucuccuuca agcccaucau cgacaaggcc cgcaccggca gccacuccga caaccugaag     2160

gagguggucg gggagcuggc cggcagcccc gcgaucaaga agggcauccu ccagucccug     2220

aagaucgugg acgagcuggu gaaggucaug ggguacgagc cggagcagau cguggucgag     2280

auggcccgcg agaaccagac cacggccaag ggccucagcc ggagccgcca gcggcugacc     2340

acccugcgcg agucccucgc caaccucaag agcaacaucc uggaggagaa gaagcccaag     2400

uacgugaagg accaggugga gaaccaccac cucuccgacg accggcuguu ccuguacuac     2460

cuccagaacg ggaaggacau guacaccgac gacgagcugg acaucgacaa ccugagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacuccau cgacaaccgc     2580

guccucguga gcuccgcgaa gaaccggggc aagagcgacg acgugcccuc cgucgagauc     2640

gugaaggacu gcaagguguu cuggaagaag cugcuggacg ccaagcucau gagccagcgc     2700

aaguacgaca accugaccaa ggccgagcgc gggggccuga cguccgacga caaggcccgg     2760

uucauccagc ggcagcucgu cgagacccgc cagaucacca agcacguggc ccggauccug     2820

gacgagcgcu ucaacaacga gcuggacagc aagggccggc gcauccggaa ggugaagauc     2880

gucacccuca aguccaaccu ggugagcaac uuccgcaagg aguucggguu cuacaagauc     2940

cgggagguga acaacuacca ccacgcgcac gacgccuacc ugaacgccgu cguggccaag     3000

gcgauccuca cgaaguaccc gcagcuggag cccgaguucg uguacggcga cuaccccaag     3060

uacaacuccu acaagacccg caagagcgcc accgagaagc uguucuucua cuccaacauc     3120

augaacuucu ucaagaccaa ggucacgcuc gccgacggga ccgugguggu caaggacgac     3180

aucgagguga acaacgacac cggcgagauc gugugggaca agaagaagca cuucgccacc     3240

guccggaagg ugcugagcua cccccaggug aacaucguca agaagacgga gauccagacc     3300

ggcggguuca gcaaggaguc cauccuggcc cacggcaaca gcgacaagcu caucccgcgc     3360

aagaccaagg acaucuaccu ggaccccaag aaguacgggg gcuucgacuc ccccaucgug     3420

gcguacagcg ugcuggucgu ggccgacauc aagaagggga aggcccagaa gcucaagacc     3480

gugacggagc ugcugggcau caccaucaug gagcgcuccc gguucgagaa gaaccccagc     3540

gccuuccucg aguccaaggg cuaccugaac auccgcgacg acaagcugau gauccucccg     3600

aaguacagcc uguucgagcu ggagaacggg cggcgccggc uccuggccuc cgcgggcgag     3660

cugcagaagg ggaacgagcu cgcccugccc acccaguuca ugaaguuccu guaccucgcc     3720

agccgcuaca acgagcugaa gggcaagccc gaggagaucg agcagaagca ggaguucguc     3780

gugcagcacg uguccuacuu cgacgacauc cugcagauca ucaacgacuu cagcaaccgg     3840

gucauccucg ccgacgccaa ccuggagaag aucaacaagc uguaccagga caacaaggag     3900

aacaucccgg uggacgagcu cgcgaacaac aucaucaacc uguucaccuu cacgucccug     3960

ggggcccccg ccgccuucaa guucuucgac aagaucgugg accgcaagcg guacaccagc     4020

accaaggagg uccucaacuc cacccugauc caccagagca ucacgggccu guacgagacc     4080

cgcaucgacc ucggcaagcu gggggaggac uga                                  4113


<210>  1983
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1983
augaagguga acaacuacca caucggccug gacaucggga ccagcuccau cggcuggguc       60

gccaucgggg aggacggcaa gccccuccgc aucaagggga agaccgccau cggcgcgcgg      120

cuguuccagg agggcaaccc ggccgccgac cgccggaugu uccgcacgac ccggcgccgg      180

cugagccgcc ggaaguggcg ccucaagcug cuggaggaga ucuucgaccc cuacaucacc      240

cccguggacu ccaccuucuu cgcccggcuc aagcagagca accugucccc caaggacagc      300

cgcaaggagu ucaagggguc caugcuguuc ccggaccuca cggacaugca guaccacaag      360

gacuacccca ccaucuacca ccuccggcac gcccugauga cccaggacga gaaguucgac      420

auccgcaugg uguaccuggc gauccaccac aucgucaagu accggggcaa cuuccugaac      480

agcacccccg uggacuccuu caaggccagc aaggugaacu ucgucgacca guucaagaag      540

cugaacgagc ucuacacggc caucaacccc gaggaguccu uccagaucaa ccuggccaac      600

agcgaggaca ucgggcacca guuccuggac ccguccaucc gcaaguucga caagaagaag      660

cagaucccca agaucgugcc cgugagcguc gacgacaagg cgaccgacaa gaucaacggc      720

aagaucgccu ccgagaucau caacgccauc cucggguaca agagcaagcu ggacguggug      780

guccagugca cccccgugga cuccaagagc ugggcccuga aguucgacga ggaggacauc      840

gacgccaagc uccagaagau ccugccggag auggacgaga accagcaguc caucaucgcg      900

auccugcaga accucuacag ccaggugacc cugaaccaga ucguccccaa cggcaugagc      960

cuguccgaga gcaugaucga gaaguacaac gaccaccacg accaccucaa gcuguacaag     1020

aagaucaucg accagcuggc cgaccccaag aagaaggccg cccucaagaa ggccuacucc     1080

caguacgugg gcgacgacgg gaaggugauc gagcaggcgg acuucuggag cuccgucaag     1140

aagaaccugg acgacagcga ccuguccaag cagaucaugg accucaucga cgccgagaag     1200

uucaugccca agcagcggac gagccagaac ggcgugaucc cgcaccagcu gcaccagcgc     1260

gagcuggacg agaucaucga gcaccagucc aaguacuacc ccuggcucgc cgagaucaac     1320

cccaacaagc acgaccugca ccuggccaag uacaagaucg aggagcucgu ggccuuccgg     1380

guccccuacu acguggggcc gaugaucacc cccgacgacc aggccaagag cgcggagacc     1440

guguucuccu ggauggagcg caagggcaag gaggccggcc agaucacccc cuggaacuuc     1500

gacgagaagg ucgaccggaa cgccagcgcc aaccgcuuca ucaagcgcau gacgaccaag     1560

gacaccuacc ugaucgggga ggacgugcug cccgacgagu cccuccugua cgagaaguuc     1620

aagguccuga acgagcucaa cauggugcgg gugaacggca agcugcugaa ggucgcggac     1680

aagcaggcca ucuuccagga ccucuucgag aacuacaagc acaucagcgu gaagaagcug     1740

cagaacuaca ucaaguccaa gaccgggcug ccgagcgacc ccgagaucuc cggccugagc     1800

gaccccgagu acuucaacaa cucccucggg acguacaacg acuucaagaa gcuguucggc     1860

aacaaggugg acgagcccga ccugcaggac gacuucgaga agaucgucga guggagcacc     1920

guguucgagg acaagcgcau ccuccgggag aagcugaacg agaucaccug gcugagcgac     1980

cagcagaagg acgugcucga guccagccgc uaccagggcu gggggcggcu guccaagaag     2040

cugcucaccg gcaucgucaa cgaccagggg gagcgcauca ucgacgagcu guggaacacg     2100

aacaagaacu ucaugcagau ccagagcgac aacgacuucg ccaagcggau ccacgaggcc     2160

aacgcggacc agaugaaggc cguggacgug gaggacgucc uggccgacgc cuacaccucc     2220

ccgcagaaca agaaggccau ccgccaggug gugaaggucg uggacgacau ccagaaggcg     2280

augggcgggg uggcccccaa guacaucagc aucgaguuca cccgguccga ggaccgcaac     2340

ccccggcgca ccaucagccg gcagcgccag cucgagaaca cgcugaagga caccgccaag     2400

ucccuggcca agagcaucaa cccggagcuc cuguccgagc uggacaacgc cgccaagagc     2460

aagaagggcc ucaccgaccg gcuguaccug uacuucaccc agcucggcaa ggacaucuac     2520

acggggaagc ccaucaacau cgacgagauc uccaccuacg acaucgacca cauccugccc     2580

caggcguucg ucaaggacga cagccugaac aaccgcgugc ucguguccaa ggccaucaac     2640

aacggcaaga gcgacaacgu ccccgugcag cuguucgggg ccaagauggg ccacuucugg     2700

aagcagcugg ccgaggcggg gcucaucucc aagcggaagc ugaagaaccu gcagaccgac     2760

ccggacacca ucagcaagua cgccaugcac ggcuucaucc gccggcagcu cguggagacg     2820

agccagguca ucaagcuggu ggccaacauc cugggcgaca aguaccgcaa cgacaacacc     2880

aagaucaucg agaucaccgc ccgcaugaac caccagaugc gggacgaguu cggguucauc     2940

aagaaccgcg agaucaacga cuaccaccac gcguucgacg ccuaccucac cgccuuccug     3000

ggccgguacc uguaccaccg cuacaucaag cuccggcccu acuucgugua cggggacuuc     3060

aagaaguuca aggaggacaa ggucaccaug cgcaacuuca acuuccugca cgaccugacg     3120

gacgacaccc aggagaagau cgccgacgcc gagaccggcg aggugaucug ggaccgggag     3180

aacuccaucc agcagcucaa ggacguguac cacuacaagu ucaugcugau cagccacgag     3240

gucuacaccc ugcgcggcgc gauguucaac cagacggugu accccgccuc cgacgccggg     3300

aagcggaagc ucauccccau caaggccgac cgcccgauca acguguacgg cggguacagc     3360

ggcuccgccg acgcguacau ggccaucguc cggauccaca acaagaaggg ggacaaguac     3420

cgcguggugg gcguccccau gcgggcccgc gaccggcugg acgccgccaa gaaggugagc     3480

gacgcggacu gcgaccgcgc ccugaaggac gugcucacgc cccagcugac caagaccaag     3540

aagucccgga agaccggcga gaucacccag gucguggagg acuucgagau cgugcugggg     3600

aaggucaugu accgccagcu caugaucgac ggcgacaaga aguucaugcu ggggagcucc     3660

accuaccagu acaacgccaa gcagcuggug cucagcgacc aguccgugaa gacccuggcc     3720

agcaagggcc ggcuggaccc ccuccaggag uccauggacu acaacaacgu cuacacggag     3780

auccuggaca aggugaacca guacuucagc cuguacgaca ugaacaaguu ccgccacaag     3840

cucaaccugg gguucuccaa guucaucagc uucccgaacc acaacguguu cgacggcaac     3900

accaaggcgu ccagcggcaa gcgggagauc cuggaggagg uccucaacgg gcugcacgcc     3960

aaccccaccu ucggcaaccu gaaggacauc gggaucacca cgcccuucgg ccagcuccag     4020

cagcccaacg ggauccugcu guccgacgag gccaagaucc gcuaccagag cccgaccggc     4080

cucuucgagc gcaccgugag ccugaaggac cuguga                               4116


<210>  1984
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1984
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcauccgg      240

uaccugcagg agaucuucuc cagcgagaug uccaaggucg acgacagcuu cuuccaccgc      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcggcaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccgcaag      420

aagcucgccg acagcaccga caaggcggac cugcggcuga ucuaccucgc ccuggcccac      480

augaucaagu uccgcggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

auggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gccgggugga cgcgaaggcc auccuguccg cccgccugag caagucccgg      660

cgccucgaga accugaucgc ccagcugccg ggcgagaagc ggaacggccu cuucgggaac      720

cugaucgccc ugagccucgg gcugacgccc aacuucaagu ccaacuucga ccuggcggag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg cgucaacagc gagaucacca aggcgccccu guccgccagc      960

augaucaagc gguacgacga gcaccaccag gaccucaccc ugcugaaggc ccucgugcgc     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc aguccaagaa cggcuacgcg     1080

ggguacaucg acggcggggc cagccaggag gaguucuaca aguucaucaa gccgauccug     1140

gagaagaugg acggcacgga ggagcuccug gccaagcuga accgcgagga ccugcuccgg     1200

aagcagcgca ccuucgacaa cggcuccauc ccccaccaga uccaccuggg ggagcugcac     1260

gccauccucc ggcgccagga ggacuucuac cccuuccuga aggacaaccg ggagaagauc     1320

gagaagaucc ugaccuuccg cauccccuac uacgugggcc cgcucgcccg ggggaacagc     1380

cgcuucgcgu ggaugacccg gaaguccgag gagacgauca cccccuggaa cuucgaggag     1440

gucguggaca agggcgccag cgcccagucc uucaucgagc gcaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu gcugcccaag cacagccucc uguacgagua cuucaccguc     1560

uacaacgagc ugacgaaggu gaaguacgug accgagggga ugcggaagcc ggaguuccuc     1620

uccggcaagc agaaggaggc caucgucgac cugcuguuca agaccaaccg caaggugacc     1680

gugaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacag cgucgagauc     1740

uccggcgugg aggaccgguu caacgcgagc cuggggacgu accacgaccu gcucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucgug     1860

cucacccuga cccuguucga ggacaaggag augaucgagg agcgccucaa gaaguacgcc     1920

aaccuguucg acgacaaggu caugaagcag cugaagcggc gccacuacac cggcuggggg     1980

cggcucuccc gcaagcugau caacggcauc cgggacaagc agagcgggaa gacgauccug     2040

gacuuccuca aguccgacgg cuucgccaac cgcaacuuca ugcagcugau caacgacgac     2100

agccugaccu ucaaggaggc cauccagaag gcccaggugu ccggccaggg gcacagccuc     2160

cacgagcaga ucgcgaaccu ggccggcagc cccgccauca agaaggggau ccugcagucc     2220

gucaaggugg uggacgagcu cgucaaggug augggccaca agcccgagaa caucgugauc     2280

gagauggccc gcgagaacca gaccacccag aaggggcaga agaacagccg ggagcgcaug     2340

aagcggaucg aggagggcau caaggagcug ggcucccaga uccugaagga gcaccccguc     2400

gagaacacgc agcuccagaa cgagaagcug uaccuguacu accuccagaa cgggcgcgac     2460

auguacgugg accaggagcu ggacaucaac cggcugagcg acuacgacgu ggaccacauc     2520

gucccgcagu ccuucaucaa ggacgacagc aucgacaaca aggugcucac ccgcuccgac     2580

aagaaccggg gcaagagcga caacgugccc uccgaggagg ucgugaagaa gaugaagaac     2640

uacuggcgcc agcugcugaa cgccaagcuc aucacccagc ggaaguucga caaccugacc     2700

aaggcggagc gcgggggccu gagcgagcuc gacaaggccg gcuucaucaa gcggcagcug     2760

guggagacgc gccagaucac caagcacguc gcccagaucc uggacucccg gaugaacacc     2820

aaguacgacg agaacgacaa gcucauccgc gaggugaagg ugaucacccu caagagcaag     2880

cuggucuccg acuuccggaa ggacuuccag uucuacaagg ugcgcgagau caacaacuac     2940

caccacgccc acgacgccua ccucaacgcg guggucggga ccgcccugau caagaaguac     3000

cccaagcugg agagcgaguu cguguacggc gacuacaagg uguacgacgu ccggaagaug     3060

aucgccaagu ccgagcagga gaucgggaag gccacggcga agcgcuucuu cuacagcaac     3120

aucaugaacu ucuucaagac cgagaucacc cucgccaacg gcgagauccg gaagcgcccc     3180

cugaucgaga ccaacgagga gacgggggag aucguguggg acaagggccg ggacuucgcc     3240

accgugcgca agguccuguc caugccccag gugaacaucg ugaagaagac cgagguccag     3300

accggcgccc ucacgaacga gagcaucuac gcccgcggga gcuucgacaa gcugaucucc     3360

cggaagcacc gcuucgagag cuccaaguac ggcggguucg gcagcccgac cgugaccuac     3420

uccgugcugg ucguggccaa gagcaaggug caggacggga aggucaagaa gaucaagacc     3480

ggcaaggagc ucaucggcau cacgcugcug gacaagcucg uguucgagaa gaacccccug     3540

aaguucaucg aggacaaggg guacggcaac gugcagaucg acaagugcau caagcugccc     3600

aaguacuccc ucuucgaguu cgagaacggg acccggcgca ugcuggccag cgucauggcc     3660

aacaacaacu cccggggcga ccugcagaag gccaacgaga uguuccuccc ggccaagcug     3720

gugacccugc ucuaccacgc gcacaagauc gagagcucca aggagcugga gcacgaggcc     3780

uacauccugg accacuacaa cgaccucuac cagcugcuga gcuacaucga gcgcuucgcc     3840

ucccucuacg uggacgucga gaagaacauc agcaagguga aggagcuguu cuccaacauc     3900

gagagcuacu ccaucagcga gaucugcucc agcgugauca accugcucac ccugacggcc     3960

agcggggccc ccgcggacuu caaguuccug ggcaccacca ucccccggaa gcgcuacggc     4020

uccccgcaga gcauccucuc cagcacccug auccaccagu ccaucacggg gcuguacgag     4080

acccggaucg accucagcca gcugggcggg gacuga                               4116


<210>  1985
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1985
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcauccgg      240

uaccugcagg agaucuucuc cagcgagaug uccaaggucg acgacagcuu cuuccaccgc      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcggcaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccgcaag      420

aagcucgccg acagcaccga caaggcggac cugcggcuga ucuaccucgc ccuggcccac      480

augaucaagu uccgcggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

auggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gccgggugga cgcgaaggcc auccuguccg cccgccugag caagucccgg      660

cgccucgaga accugaucgc ccagcugccg ggcgagaagc ggaacggccu cuucgggaac      720

cugaucgccc ugagccucgg gcugacgccc aacuucaagu ccaacuucga ccuggcggag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg cgucaacagc gagaucacca aggcgccccu guccgccagc      960

augaucaagc gguacgacga gcaccaccag gaccucaccc ugcugaaggc ccucgugcgc     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc aguccaagaa cggcuacgcg     1080

ggguacaucg acggcggggc cagccaggag gaguucuaca aguucaucaa gccgauccug     1140

gagaagaugg acggcacgga ggagcuccug gccaagcuga accgcgagga ccugcuccgg     1200

aagcagcgca ccuucgacaa cggcuccauc ccccaccaga uccaccuggg ggagcugcac     1260

gccauccucc ggcgccagga ggacuucuac cccuuccuga aggacaaccg ggagaagauc     1320

gagaagaucc ugaccuuccg cauccccuac uacgugggcc cgcucgcccg ggggaacagc     1380

cgcuucgcgu ggaugacccg gaaguccgag gagacgauca cccccuggaa cuucgaggag     1440

gucguggaca agggcgccag cgcccagucc uucaucgagc gcaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu gcugcccaag cacagccucc uguacgagua cuucaccguc     1560

uacaacgagc ugacgaaggu gaaguacgug accgagggga ugcggaagcc ggaguuccuc     1620

uccggcaagc agaaggaggc caucgucgac cugcuguuca agaccaaccg caaggugacc     1680

gugaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacag cgucgagauc     1740

uccggcgugg aggaccgguu caacgcgagc cuggggacgu accacgaccu gcucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucgug     1860

cucacccuga cccuguucga ggacaaggag augaucgagg agcgccucaa gaccuacgcc     1920

caccuguucg acgacaaggu caugaagcag cugaagcggc gccacuacac gggcuggggg     1980

cggcucuccc gcaagcugau caacggcauc cgggacaagc agagcgggaa gaccauccug     2040

gacuuccuca aguccgacgg cuucgccaac cgcaacuuca uccagcugau ccacgacgac     2100

agccugaccu ucaaggaggc cauccagaag gcccaggugu ccggccaggg gcacagccuc     2160

cacgagcaga ucgcgaaccu ggccggcagc cccgccauca agaaggggau ccugcagucc     2220

gucaaggugg uggacgagcu cgucaaggug augggccaca agcccgagaa caucgugauc     2280

gagauggccc gcgagaacca gaccacgcag aaggggcaga agaacagccg ggagcgcaug     2340

aagcggaucg aggagggcau caaggagcug ggcucccaga uccugaagga gcaccccguc     2400

gagaacaccc agcuccagaa cgagaagcug uaccuguacu accuccagaa cgggcgcgac     2460

auguacgugg accaggagcu ggacaucaac cggcugagcg acuacgacgu ggaccacauc     2520

gucccgcagu ccuucaucaa ggacgacagc aucgacaaca aggugcucac ccgcuccgac     2580

aagaaccggg gcaagagcga cgacgugccc uccgaggagg ucgugaagaa gaugaagaac     2640

uacuggcgcc agcugcugaa cgccaagcuc aucacccagc ggaaguucga caaccugacg     2700

aaggcggagc gcgggggccu gagcgagcuc gacaaggccg gcuucaucaa gcggcagcug     2760

guggagaccc gccagaucac caagcacguc gcccagaucc uggacucccg gaugaacacc     2820

aaguacgacg agaacgacaa gcucauccgc gaggugaagg ugaucacccu caagagcaag     2880

cuggucuccg acuuccggaa ggacuuccag uucuacaagg ugcgcgagau caacaacuac     2940

caccacgccc acgacgccua ccucaacgcg guggucggga cggcccugau caagaaguac     3000

cccaagcugg agagcgaguu cguguacggc gacuacaagg uguacgacgu ccggaagaug     3060

aucgccaagu ccgagcagga gaucgggaag gccaccgcga agcgcuucuu cuacagcaac     3120

aucaugaacu ucuucaagac cgagaucacc cucgccaacg gcgagauccg gaagcgcccc     3180

cugaucgaga cgaacgagga gaccggggag aucgugugga acaagggccg ggacuucgcc     3240

accgugcgca agguccuguc caugccccag gugaacaucg ugaagaagac cgagguccag     3300

acgggcgccc ucaccaacga gagcaucuac gcccgcggga gcuucgacaa gcugaucucc     3360

cggaagcacc gcuucgagag cuccaaguac ggcggguucg gcagcccgac cgugaccuac     3420

uccgugcugg ucguggccaa gagcaaggug caggacggga aggucaagaa gaucaagacg     3480

ggcaaggagc ucaucggcau cacccugcug gacaagcucg uguucgagaa gaacccccug     3540

aaguucaucg aggacaaggg guacggcaac gugcagaucg acaagugcau caagcugccc     3600

aaguacuccc ucuucgaguu cgagaacggg acccggcgca ugcuggccag cgucauggcc     3660

aacaacaacu cccggggcga ccugcagaag gccaacgaga uguuccuccc ggccaagcug     3720

gugacccugc ucuaccacgc gcacaagauc gagagcucca aggagcugga gcacgaggcc     3780

uacauccugg accacuacaa cgaccucuac cagcugcuga gcuacaucga gcgcuucgcc     3840

ucccucuacg uggacgucga gaagaacauc agcaagguga aggagcuguu cuccaacauc     3900

gagagcuacu ccaucagcga gaucugcucc agcgugauca accugcucac gcugaccgcc     3960

agcggggccc ccgcggacuu caaguuccug ggcaccacca ucccccggaa gcgcuacggc     4020

uccccgcaga gcauccucuc cagcacgcug auccaccagu ccaucaccgg gcuguacgag     4080

acccggaucg accucagcca gcugggcggg gacuga                               4116


<210>  1986
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1986
augaacaaca acaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucaccg acgacuacaa ggugcccagc aagaagaugc gcgugcucgg gaacacggac      120

aagcgguuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcg      180

gaggaccgcc ggcugaagcg caccgcccgg cgccgguaca cgcgccggaa gaaccgccug      240

cgguaccucc aggagaucuu cgccgaggag auguccaagg ucgacagcuc cuucuuccac      300

cgccuggacg acagcuuccu ggugccggag gacaaguccg gcagcaagua ccccaucuuc      360

gccacccucg ccgaggagaa ggaguaccac aagaaguucc ccaccaucua ccaccuccgg      420

aagcaccugg cggacuccaa ggagaaggcc gaccuccgcc ugaucuaccu ggcccucgcc      480

cacaucacca aguaccgggg ccacuuccug uacgaggagg cguucgacau caagaacaac      540

gacauccaga agaucuucaa cgaguucauc aacaucuacg acaacacguu cgaggggagc      600

ucccugagcg gccagaacgc ccagguggag gccaucuuca ccgggaagau cuccaagagc      660

gucaagcgcg agcacgugcu caagcuguuc cccgacgaga aguccaccgg ccuguucagc      720

gaguuccuca agcugaucgu ggggaaccag gccgacuuca agaagcacuu cgaccuggag      780

gagaaggccu cccuccaguu cagcaaggac accuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gcgacgacuu cgcggaccug uuccuggucg ccaagaagcu cuacgacgcc      900

auccugcugu ccgggauccu cacggugacc gacccgagca ccaaggcgcc ccugagcgcc      960

uccaugaucg agcgguacga gaaccaccag gaggaccugg ccgcccucaa gcaguucauc     1020

aagaacaacc ugcccgagaa guacgccgag guguucagcg accaguccaa ggacggcuac     1080

gccggguaca ucgacggcaa gaccacgcag gaggccuucu acaaguacau caagaaccug     1140

cucagcaagu ucgagggcgc ggacuacuuc cuggacaaga ucgagcgcga ggacuuccug     1200

cggaagcagc gcaccuucga caacgggucc aucccccacc agauccaccu ccaggagaug     1260

aacgccaucc ugcggcgcca gggcgagcac uacccgcugc ucaaggagaa caaggagaag     1320

aucgagaaga uccugaccuu ccgcaucccc uacuacgucg ggccccuggc ccggggcaac     1380

cgcgacuucg ccuggcucac ccggaacagc gaccaggcca uccgccccug gaacuucgag     1440

gagaucgugg acaaggcguc cagcgccgag uccuucauca acaagaugac gaacuacgac     1500

cuguaccugc cggaggagaa ggugcugccc aagcacagcc uccuguacga gaccuucgcc     1560

gucuacaacg agcugaccaa ggugaaguuc aucgccgagg ggcuccggga cuaccaguuc     1620

uucgacuccg gccagaagaa gcagaucgug aaccagcugu ucaaggagaa gcgcaagguc     1680

accgagaagg acaucaucca cuaccugcac aacguggacg gcuacgacgg gaucgagcuc     1740

aagggcaucg agaagcaguu caacgcgagc cuguccacgu accacgaccu gcucaagauc     1800

aucaaggaca aggaguucau ggacaaccac aagaaccagg agauccugga gaacaucgug     1860

cacacccuga ccaucuucga ggaccgggag augaucaagc agcgccucgc ccaguacgac     1920

agcaucuucg acgagaaggu caucaaggcc cugacccggc gccacuacac gggguggggc     1980

aagcuguccg ccaagcucau caacgggauc ugcgacaaga agaccggcaa gaccauccug     2040

gacuaccuga ucgacgacgg cuacaacaac cggaacuuca ugcagcucau caacgacgac     2100

gggcugagcu ucaaggagau cauccagaag gcccaggugg ugggcaagac cgacgaccug     2160

acgcaggucg ugcgcgagcu cuccgggagc cccgcgauca agaagggcau ccugcagucc     2220

aucaagaucg ucgacgagcu ggugaagauc augggguacg cccccgagag caucgugauc     2280

gagauggccc gggagaacca gaccaccgcc aagggcaaga agaacagcca gcagcgcuac     2340

aagcggaucg aggacgcccu caagaaccug gcgccgggcc uggacuccac caucagcaag     2400

gagaacccca cggacaacau ccagcuccag aacgaccgcc uguuccugua cuaccuccag     2460

aacgggaagg acauguacac cggcgaggcc cuggacauca accagcuguc cagcuacgac     2520

aucgaccaca ucauccccca ggccuucauc aaggacgacu cccucgacaa ccggguccug     2580

accagcucca aggacaaccg cggcaagagc gacaacgugc ccucccugga gguggucaag     2640

aagcggaagg ccuucuggca gcagcuccug gacagcaagc ugaucuccga gcgcaaguuc     2700

aacaaccuca ccaaggccga gcgcgggggc cuggacgagc gggacaaggu gggguucauc     2760

aagcgccagc ugguggagac ccggcagauc acgaagcacg ucgcgcgcau ccucgacgcc     2820

cgguucaaca ccgaggugag cgagaagaac cagaagaucc gcuccgugaa gaucaucacc     2880

cucaagagca accuggucuc caacuuccgg aaggaguuca agcuguacaa ggugcgcgag     2940

aucaacgacu accaccacgc ccacgacgcc uaccugaacg cgguggucgc caaggccauc     3000

cugaagaagu acccgaagcu cgagcccgag uucguguacg gcgacuacca gaaguacgac     3060

cugaagcggu acaucagcaa guccaaggac cccaaggagg uggagaaggc caccgagaag     3120

uacuucuucu acagcaaccu gcucaacuuc uucaaggagg agguccacua cgccgacggg     3180

acgaucguga agcgcgagaa caucgaguac agcaaggaca ccggcgagau cgcguggaac     3240

aaggagaagg acuucgugac caucaagaag guccuguccu acccccaggu gaacaucgug     3300

aagaagcggg agguccagac cgggggcuuc agcaaggagu ccauccugcc gaagggcaac     3360

agcgacaagc ucaucccccg caagacgaag gacauccugu gggacaccac caaguacggg     3420

ggcuucgacu cccccgugau cgccuacagc auccugcuca ucgccgacau cgagaagggg     3480

aaggccaaga agcugaagac cgugaagacg cuggucggca ucaccaucau ggagaaggcc     3540

gcguucgaga agaaccccau caccuuccuc gagaacaagg gguaccacaa cgugcggaag     3600

gagaacaucc ugugccugcc gaaguacucc cucuucgagc uggagaacgg ccgccggcgc     3660

cugcucgcca gcgccaagga gcugcagaag ggcaacgaga ucgugcugcc cgucuaccuc     3720

accacgcugc uguaccacuc caagaacgug cacaagcucg acgagcccga gcaccuggag     3780

uacauccaga agcaccggua cgaguucaag gaccugcuca accuggugag cgaguucucc     3840

cagaaguacg uccuggccga ggccaaccuc gagaagauca agagccugua cguggacaac     3900

gagcaggcgg acaucgagau ccuggccaac uccuucauca accuccugac cuucaccgcc     3960

cugggggccc cggcggccuu caaguucuuc ggcaaggacg uggaccgcaa gcgguacacc     4020

acggucagcg agauccucaa cgccacccug auccaccagu ccaucaccgg gcuguacgag     4080

acccgcaucg accucagcaa gcugggcgag gacuga                               4116


<210>  1987
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1987
augaccgaga agaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucacgg acgacuacaa ggugcccgcc aagaagauga aggugcucgg gaacaccgac      120

aagaaguaca ucaagaagaa ccugcugggc gcgcuccugu ucgacaacgg ggagaccgcc      180

gaggccaccc gccugaagcg gacggcccgc cggcgcuaca cccggcgcaa gaaccggcuc      240

cgcuaccugc aggagaucuu cgccgaggag auggcgaagg ucgacgagag cuucuucuac      300

cggcuggacg aguccuuccu caccaccgac gacaaggacu ucgagcgcca cccgaucuuc      360

ggcaacaagg ccgacgagau caaguaccac caggaguucc ccacgaucua ccaccuccgg      420

aagcaccugg ccgacagcca cgagaaggcc gaccuccgcc ugaucuaccu ggcgcucgcc      480

cacaugauca aguuccgggg ccacuuccug aucgaggggg agcugaacgc cgagaacacc      540

gacgugcaga agcuguucga ggccuucgug gaggucuacg accgcaccuu cgacgacucc      600

aaccugagcg agaucaccgu ggacgccucc agcauccuga cggagaaguu cuccaagagc      660

cggcgccucg agaaccugau caagcacuac cccaccgaga agaagaacac ccuguucggc      720

aaccucgugg cgcuggcccu ggggcuccag cccaacuuca agaccuccuu caagcugagc      780

gaggacgcca agcugcaguu cuccaaggac acguacgagg aggaccucga ggagcugauc      840

ggcaagaucg gggacgagua cgccgaccug uucaccagcg ccaagaaccu cuacgacgcg      900

auccugcugu ccggcauccu caccgucgcc gacaacacca cgaaggcccc gcugagcgcc      960

uccaugauca agcgguacaa cgagcaccag guggaccuga agaagcucaa ggaguucauc     1020

aagaacaacg ccagcgacaa guacgacgag aucuucaacg acaaggacaa gaacggcuac     1080

gcgggguaca ucgagaacgg cgugaagcag gacgaguucu acaaguaccu gaagaccacc     1140

cuguccaaga ucgacgggag cgacuacuuc cucgacaaga ucgagcgcga ggacuuccug     1200

cggaagcagc gcaccuucga caacggcagc aucccccacc agauccaccu ccaggagaug     1260

cacgccaucc uccggcgcca gggcgagcac uacgccuucc ugaaggagaa ccaggccaag     1320

aucgagaaga uccugacguu ccgcaucccc uacuacgucg ggccccucgc ccggaagaac     1380

ucccgcuucg cgugggccga guaccacagc gacgagaaga ucaccccgug gaacuucgac     1440

gagaucaucg acaaggagaa guccgccgag aaguucauca cccggaugac ccugaacgac     1500

cuguaccugc ccgaggagaa ggugcucccc aagcacagcc ugguguacga gacguacacc     1560

gucuacaacg agcugaccaa ggugaaguac gugaacgagc agggcaaguc caacuucuuc     1620

gacgccaaca ugaagcagga gaucuucgag cacgucuuca aggagaaccg caaggugacc     1680

aaggacaagu uccucaacua ccugaacaag gaguuccccg aguaccggau ccaggaccug     1740

aucgggcucg acaaggagaa caagagcuuc aacgcguccc ugggcacgua ccacgaccug     1800

aagaagaucc ucgacaagag cuuccuggac gacaagacca acgagaccau caucgaggac     1860

aucauccaga cccugacgcu guucgaggac cgcgacauga uccggcagcg ccugcagaag     1920

uacuccgaca ucuucacccc gcagcagcug aagaagcucg agcggcgcca cuacaccggg     1980

uggggccggc ugagcuacaa gcugaucaac ggcauccgca acaaggagaa cgggaagucc     2040

auccucgacu accugaucga cgacggcuac gccaaccgga acuucaugca gcugaucagc     2100

gacgacaccc uccccuucaa gcagaucauc aaggacgccc agaucaucgg ggacaucgac     2160

gacgugaccu ccgucgugcg cgagcugccc ggcagccccg ccaucaagaa ggggauccug     2220

caguccguga agaucgucga cgagcucgug aaggucaugg gccacaaccc ggacaacauc     2280

gugaucgaga uggcccggga gaaccagacg accaaccgcg gccggaacca gagccagcag     2340

cgccugaaga agcugcagga cucccucaag gagcugggga gcaacauccu gaacgaggag     2400

aagcccuccu acaucgaggg caagguggag aacaaccacc uccaggacga ccggcuguuc     2460

cuguacuaca uccagaacgg gaaggacaug uacaccggcg acgagcucga caucgaccac     2520

cugagcgacu acgacaucga ccacaucauc ccccaggcgu ucaucaagga cgacagcauc     2580

gacaaccgcg uccugaccuc cagcgccaag aaccgcggga aguccgacga cgugcccagc     2640

cucgacaucg ugcacgaccg gaaggccgac uggauccgcc uguacaaguc cggccugauc     2700

agcaagcgga aguucgacaa ccucacgaag gccgagcgcg gcgggcugac cgagaacgac     2760

aaggccggcu ucaucaagcg gcagcugguc gagacccgcc agaucaccaa gcacguggcg     2820

cagauccucg acucccgguu caacacggag cgcgacgaga acgacaaggu gauccggaac     2880

gucaagguga ucacccucaa gagcaaccug gugucccagu uccgcaagga cuucaaguuc     2940

uacaaggucc gggagaucaa cgacuaccac cacgcccacg acgccuaccu gaacgccgug     3000

guggggaccg cccugcugaa gaaguacccg aagcucgcgc ccgaguucgu cuacggcgag     3060

uacaagaagu acgacauccg caaguucauc accagcuccg gcgacaaggc cacggccaag     3120

uacuucuucu acagcaaccu gaugaacuuc uucaagcggg ugauccgcua cuccaacggg     3180

aagguggucg ugcggcccgu gaucgagugc agcaaggaca ccggcgagau cgccuggaac     3240

aagcagaccg acuucgagaa gguccgccgg gugcuguccu acccccaggu gaacaucguc     3300

aagaaggugg agacccagac cgggggcuuc agcaaggagu ccauccuccc gaaggggaac     3360

agcgacaagc ugaucccccg caagacgaag aaguuccggu gggacacccc caaguacggc     3420

ggcuucgaca gccccaacau cgcguacucc guguucguca ucgccgacgu ggagaagggg     3480

aaggccaaga agcugaagac cgugaaggag cucgucggca ucagcaucau ggagcgcucc     3540

agcuucgagg agaacccggu gguguuccug gagaagaagg gguaccagaa cguccaggag     3600

gacaaccuga ucaagcuccc caaguacucc cuguucgagu ucgagggcgg gcggcgccgc     3660

cugcucgcca gcgccuccga gcugcagaag ggcaacgagg uggugcugag ccggcaccuc     3720

gucgagcugc uguaccacgc gcaccgcgug aacuccuuca acaacagcga gcaccucaag     3780

uacguguccg agcacaagaa ggaguucggc gagguccuga gcugcgugga gaacuucgcc     3840

aaguccuacg uggacgucga gaagaaccug gggaagaucc gggccguggc cgacaagauc     3900

gacacguuca gcaucgagga caucuccauc agcuucguga accuccugac ccugaccgcc     3960

cucggcgcgc ccgccgacuu caacuuccug ggggagaaga ucccgcgcaa gcgguacacc     4020

uccacgaagg agugccugaa cgccacccuc auccaccaga gcaucaccgg ccuguacgag     4080

acccgcaucg accuguccaa gcucggggag gaguga                               4116


<210>  1988
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1988
augaaccugc gcaacgugga gagcuaccag gucggccucg acaucgggac cggcuccgug       60

gggugggccg ugcuggacga caacggcgac cugugccggu ucaaggggaa gcccaccugg      120

ggcagccgcg ucuucccgac ggccgagacc gcggccgagg cccgggugca ccgcggccag      180

cggcgccggu acgaccgccg gcgccagcgg aucgaccucc ugcagcgcuu cuucgccgac      240

gagguggcca aggucgaccc cgaguucuuc auccggcuga accaggcgcg ccuccacccc      300

gagaaccggg accccgccca cgccgacuac cgcuggccgc uguucaacgg guccaacuuc      360

accgagaagg acuacuaccg gcaguucccc accaucuacc accuccgcgc ccaccuccuc      420

aagagcgacg agcccgcgga cauccggcug guguaccugg cccugcacaa caucgugaag      480

caccgcggca acuuccugca ccaggacaac cccucccuga gcgcccggaa cgccaacguc      540

ggggacgccg uggaggcgcu gugccuggcc cucgacgagu ggugcggcgc ccacgacgug      600

gagugcgccu gcgacgaggc cgcgcuggcc gaggcccugg gggacgccuc ccuccgcccg      660

ggcgagaagc gcgagcgggc cgaggcggcc cugggcgucg agaaggccca caagaagcug      720

gccaaggccg ugagcuccgc gguggucggg uacgccgccg aguucggcca cguguucuuc      780

gccgagagcg agggguccaa guucgcgcuc agcaacgacg agaaggugga ggccuacguc      840

ugccccgacg agggccgcga ccuguucgac gccaugcggg ccgugcacuc cagcuacgug      900

cugaugggca uccucuccga ggcccagggg gagcugcuga gcgucugcaa ggugggcgag      960

uacgagcgcu acaagcggga ccucgagacg cugaaguccc uggugcgcgc guacgggccc     1020

gagcgguacg acgaguucuu cugcggcccc uucuacgagg ggacccgcga cuacgacccg     1080

ggcaaggccc agggcuacac caaguacaac cucggggcca gcaagcuguc ccacgaggac     1140

uuccugaagg agguccagaa gcuccugaag gacaucggcg cccaggccga cgagcgguac     1200

gcggacaucg agcgcgccau cgaggccggg gccuuccugc ggcgccucaa gaccagcgac     1260

aacggcacga uccccuacca gcugcaccug gaggagaugg acgccaucau cgagcggcag     1320

gcggccuucu accccuuccu cgcccaggag aaggacaagc ucaagagccu gguguccuuc     1380

cgcauccccu acuacgucgg gccgcugacc cagaagaacg ccgcgcggga cggcaagggc     1440

gaggcccgcu ucgccuggag cucccggaag cccgggaugg aggacgcccg cguguacccc     1500

uggaacuggg aggacgugau cgacaaggac cagagcgccg aggacuucau ccggcgcaug     1560

accgggaccu gcacguaccu gaagggggag cccguccugg cgcgcugcuc ccucgacuac     1620

gagcuguucu gcgugcugaa cgagcuggcc ggcgcccggu ggacccagga cggggacaag     1680

agcuaccgcu ucgacgccgc cgaccggcuc ggcaucgugg aggaccuguu caagaagcgc     1740

aagcggguca ccuacgaggc ggccgagaag uggcuggccc gcacccacgg cccggagucc     1800

ggcaagagcg ccggcggggc cuaccacgug uccggcggcc agggggagga cgcguucgag     1860

agccagcucu ccagccacua cgacuucugc aagauccugg gcguggacga gcuguccgac     1920

gccgacgagg ccauggccga ggacaucauc cucuggaaca cggucuucga ggaccggagc     1980

auccugaagc gcaagaucaa gcuggcguac ggggaccggc ucggcgacgc ccaggugcgc     2040

aagcugugcc agaagcggua caccgggugg ggccgccugu ccggcgagcu ccugcggggg     2100

cugcgcagcg ccaccgacaa cggccccaag accaucaugg acauccuccu ggagggggac     2160

cccaaccacg cccggcgcau gggcgccgcg augaaccuga uggagauccu ccacgacgac     2220

cugcuggagu uccaggccaa gaucgacgag cacaaccggg agcgcgcccu cgccgucggg     2280

ggcauguccg uggacgaggu gacgggcagc cccgcccugc ggcgcgggau caaccaggcg     2340

cugcgggugg ucgacgagau cgccggcauc gccgggaagc cgcccgccca caucuacgug     2400

gaggugaccc gcgacgaggg cgcccgcaac aaggggcggc gcaccaccuc ccgguaccgc     2460

gcgcucgagg ccgcccugag cagcaucaag gcccagugcg ccgacgucua cgcggagcug     2520

aaggagcggg acccccgcgc ccucgacgac gagcggcuga cgcuguacuu caugcagugc     2580

ggcaagugca uguacuccgg cgacgcccuc gacaucaaca agcugcagac cuaccaggug     2640

gaccacaucc ugccccagag cuacgugaag gacgacuccu ucgagaacaa gguccucgug     2700

cgcccggaca ccaaccagcg gaagggggac acccugcugc ucgacgagcg ccugaucgcc     2760

cggaugcgcg gcuucuggcg gacgcugcgc gacgcggagc ucaucgggga gaagaaguuc     2820

aacaaccuga cccggaccag cguguccgac gagcagauga agaaguucgu cagccgccag     2880

cugguggaga ccggccagau ggugaagcag guccagcagc uccugcaggc ccgguacccc     2940

gaguccgagg ugcagcccgu gaaggccggg gucagccacc agcugcgcgu gggcugcgcc     3000

cucgccaagu cccgggagau caacgacuuc caccacgcgc acgacgccua ccuggccugc     3060

caggugggcc gcuucgucca guaccgccac agcgccgugu acuccgagcc ggugaagaug     3120

gccgcggucg ugaagcaguu caucaagaag caggccgagg acuaccggcg cacgcgggag     3180

cugcccggga gcgacggcuu caucgugucc agcuucauga cccccggguu cgacgucaag     3240

accggcgagg uguuccgcga cgccugggac gccggcgccg agaucgaccg gauccgccgg     3300

ugcuucgacu acaaggacug cuucaucucc cgcaugcccg aggagaccca cggggcguuc     3360

ugggacgcca cgaucuacag cccgcgggcc gccggcaaga cccucucccu gccccugaag     3420

cgcgggcucg acccccggaa guacggcagc uacagccgcg agcaguucgc guacuucuuc     3480

guguacgagg ccgucaagcc caagaagaag cagcgggugc uggaguucgc cccggugccc     3540

guccgcgugg ccuccgcccu ggcgaacgac cccgacgccc ucgaggacua cgcccgggag     3600

cuggccgagg ccgaggggcu gguguucgag cgcauccggc gccggaaggu cuacaaguac     3660

cagcagauca ucgugggcga cagccgccuc uacgugaccg gccgggagga gguccgcaac     3720

gcgcgccagc uggccuucuc ccaggacgag accaagcuga ucugccggau cgagcagggg     3780

gaggagugcg aggagggcga gcuccuggac cuguuccgca gcaugcagcg gaaguaccuc     3840

gccugcgcgc cccgccuggc ccggcugaug aagguggccg agauggagga gggguucauc     3900

gccgccaccg ccuccggcaa gcgcgagguc cuccugagcc ugacguccau cgcggccgcc     3960

caccggaaca gcaucgaccu caagccgauc gggcgcaagg gcuccagcgg ccagaugaag     4020

aucaccuucu ccaaggagcu gagcaccaac gggauccugu ucguggacuc cagcgugacc     4080

ggcauguucg agcggcgcug ccggcucggg cuguga                               4116


<210>  1989
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1989
auggagaaca agaacuacag caucggccug gacaucggga ccaacuccgu gggcuggagc       60

gucaucaccg acgacuacaa ggugcccucc aagaagauga aggugcucgg gaacacggac      120

aagcgcuuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcc      180

gaggcgcggc gccugaagcg gaccgcccgc cggcgcuaca cgcggcgcaa gaaccggcug      240

cgcuaccucc aggagaucuu cgccgaggag augagcaagg ucgacuccag cuucuuccac      300

cggcuggacg acuccuuccu gaucccggag gacaagcgcg gcagcaagua ccccaucuuc      360

gccacccucg ccgaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgg      420

aagcagcugg cggacuccaa ggagaagacc gaccuccgcc ugaucuaccu ggcccucgcc      480

cacaugauca aguaccgggg ccacuuccug uacgaggaca cguucgacau caagaacaac      540

gacauccaga agaucuucag cgaguucauc uccaucuacg acaacaccuu cgaggggagc      600

ucccugagcg gccagaacgc ccagguggag gcgaucuuca ccgacaagau cuccaagagc      660

gccaagcgcg agcgggugcu caagcuguuc cccgacgaga aguccaccgg gcuguucagc      720

gaguuccuca agcugaucgu cggcaaccag gccgacuuca agaagcacuu cgaccugggg      780

gagaaggccc cgcuccaguu cuccaaggac acguacgacg aggaccugga gaaccugcuc      840

ggccagaucg gcgacgacuu cgccgaccug uuccuggugg cgaagaagcu cuacgacgcc      900

auccugcuga gcgggauccu caccgugacc gaccccagca ccaaggcgcc ccuguccgcc      960

agcaugaucg agcgcuacga gaaccaccag aaggaccugg ccguccucaa gcaguucauc     1020

aagaacaacc ugcccgagaa guacgacgag guguucuccg accagagcaa ggacggcuac     1080

gccggguaca ucgacggcaa gacgacccag gaggccuucu acaaguacau caagaaccug     1140

cucuccaagu ucgagggcac cgacuacuuc cuggagaaga ucgagcggga ggacuuccug     1200

cgcaagcagc ggaccuucga caacgggagc aucccgcacc agauccaccu ccaggagaug     1260

aacgccaucc ugcgccgcca gggcgagcac uaccccuucc ugaaggacaa caaggagaag     1320

aucgagaaga uccucacguu ccggaucccc uacuacgugg ggccccuggc ccgcggcaac     1380

cgggacuucg ccuggcugac ccgcaacucc gacgaggcga uccggccgug gaacuucgag     1440

gagaucgucg acaaggccag cuccgccgag gacuucauca acaagaugac caacuacgac     1500

cucuaccugc ccgaggagaa ggugcugccc aagcacagcc ugcucuacga gaccuucgcc     1560

guguacaacg agcugacgaa ggucaaguuc aucgccgagg ggcugcgcga cuaccaguuc     1620

cucgacuccg gccagaagaa gcagaucgug acccagcugu ucaaggagaa gcggaaggug     1680

accgagaagg acaucaucca guuccugcac aacgucgacg gcuacgacgg gaucgagcuc     1740

aagggcaucg agaagcaguu caacgcgagc cuguccaccu accacgaccu gcucaagauc     1800

aucaaggaca aggaguucau ggacgacagc aagaacgagg agauccugga gaacaucgug     1860

cacacgcuga ccaucuucga ggaccgcgag augaucaagc agcggcucgc ccaguacgac     1920

ucccuguucg acgagaaggu gaucaaggcc cugauccgcc ggcacuacac cggguggggc     1980

aagcucagcg ccaagcugau cgacgggauc ugcgacaagc agaccggcaa cacgauccug     2040

gacuaccuca ucgacgacgg caagaacaac cgcaacuuca ugcagcugau caacgacgac     2100

gggcuguccu ucaaggagau cacccagaag gcgcaggucg ugggcaagac cgacgacgug     2160

aagcaggucg ugcaggagcu ccccgggagc ccggccauca agaagggcau ccugcagucc     2220

aucaagaucg ucgacgagcu ggugaaggug auggggcaca cccccgagag caucgucauc     2280

gagauggccc gggagaacca gacgaccgcc cgcggcaaga agaacagcca gcagcgguac     2340

aagcgcaucg aggacgcccu caagaaccug gcguccggcc uggacagcaa cauccucaag     2400

gagcacccca ccaacaacau ccagcugcag aacgaccggc uguuccucua cuaccugcag     2460

aacgggcgcg acauguacac cggcaagccc cuggacauca accagcucuc cagcuacgac     2520

aucgaccaca ucgugccgca ggccuucauc aaggacgacu cccuggacaa ccgggugcug     2580

accagccuca aggacaaccg cgggaagucc gacaacgucc ccagcaucga gguggugcag     2640

aagcgcaagg ccuucuggca gcagcugcug gacuccaagc ucaucagcga gcggaaguuc     2700

aacaaccuga cgaaggccga gcgcggcggc cuggacgagc gggacaaggu cggguucauc     2760

aagcgccagc ucguggagac ccggcagauc accaagcacg uggcccagau ccuggacgcg     2820

cgcuucaaca aggaggucaa cgagaaggac aagaagaacc ggaccgugaa gaucaucacg     2880

cugaagucca accucgugag caacuuccgc aaggaguucc ggcuguacaa gguccgcgag     2940

aucaacgacu accaccacgc ccacgacgcc uaccugaacg ccgugguggc caaggcgauc     3000

cucaagaagu accccaagcu ggagcccgag uucgucuacg gcgacuacca gaaguacgac     3060

cugaagcggu acaucucccg cagccggaac ccgaaggagg uggagaaggc caccgagaag     3120

uacuucuucu acuccaaccu ccugaacuuc uucaaggagg aggugcacua cgccgacggg     3180

accaucguca agcgcgagaa caucgaguac agcaaggaca ccggcgagau cgccuggaac     3240

aaggagaagg acuucgcgac gaucaagaag gugcuguccu acccccaggu gaacaucguc     3300

aagaagcggg aggugcagac cgggggcuuc agcaaggaga gcauccuccc caagggcaac     3360

uccgacaagc ugaucccccg caagaccaag gacauccugu gggagaccac gaaguacggg     3420

ggcuucgaca gcccggugau cgccuacucc auccuccuga ucgccgacau cgagaagggg     3480

aaggccaaga agcugaagac cgucaagacc cucgugggca ucaccaucau ggagaaggcc     3540

gcguucgagg agaaccccau cacguuccug gagaacaagg gguaccacaa cgugcggaag     3600

gagaacaucc ugugccuccc caaguacagc cuguucgagc uggagaacgg ccgccggcgc     3660

cuccuggccu ccgccaagga gcugcagaag ggcaacgaga ucguccuccc gguguaccug     3720

accacccugc ucuaccacag caagaacgug cacaagcugg acgagcccga gcaccuggag     3780

uacauccaga agcaccggaa cgaguucaag gaccuccuga accuggucuc cgaguucagc     3840

cagaaguacg ugcucgccga cgccaaccug gagaagaucc agaaccugua cgcggacaac     3900

gagcaggccg acaucgagau ccucgccaac uccuucauca accugcugac cuucacggcc     3960

cucggggccc ccgcggccuu caaguucuuc ggcaaggaca ucgaccgcaa gcgcuacacc     4020

accgugagcg agauccugaa cgccacccug auccaccagu ccaucacggg gcucuacgag     4080

acccggaucg accugagcaa gcugggcgag gacuga                               4116


<210>  1990
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1990
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cuccucuucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcauccgg      240

uaccugcagg agaucuucuc cagcgagaug uccaaggucg acgacagcuu cuuccaccgc      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcggcaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccgcaag      420

aagcucgccg acagcaccga caaggcggac cugcggcuga ucuaccucgc ccuggcccac      480

augaucaagu uccgcggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

auggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gccgggugga cgcgaaggcc auccuguccg cccgccugag caagucccgg      660

cgccucgaga accugaucgc ccagcugccg ggcgagaagc ggaacgggcu guucggcaac      720

cugaucgccc ugagccucgg gcugacgccc aacuucaagu ccaacuucga ccuggcggag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg cgucaacagc gagaucacca aggcgccccu guccgccagc      960

augaucaagc gguacgacga gcaccaccag gaccucaccc ugcugaaggc ccucgugcgc     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc aguccaagaa cggcuacgcg     1080

ggguacaucg acggcggggc cagccaggag gaguucuaca aguucaucaa gccgauccug     1140

gagaagaugg acggcacgga ggagcuccug gccaagcuga accgcgagga ccugcuccgg     1200

aagcagcgca ccuucgacaa cggcuccauc ccccaccaga uccaccuggg ggagcugcac     1260

gccauccucc ggcgccagga ggacuucuac cccuuccuga aggacaaccg ggagaagauc     1320

gagaagaucc ugaccuuccg cauccccuac uacgugggcc cgcucgcccg ggggaacagc     1380

cgcuucgcgu ggaugacccg gaaguccgag gagacgauca cccccuggaa cuucgaggag     1440

gucguggaca agggcgccag cgcccagucc uucaucgagc gcaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu gcugcccaag cacagccucc uguacgagua cuucaccguc     1560

uacaacgagc ugacgaaggu gaaguacgug accgagggga ugcggaagcc ggaguuccuc     1620

uccggcaagc agaaggaggc caucgucgac cugcuguuca agaccaaccg caaggugacc     1680

gugaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacag cgucgagauc     1740

uccggcgugg aggaccgguu caacgcgagc cuggggacgu accacgaccu gcucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucgug     1860

cucacccuga cccuguucga ggacaaggag augaucgagg agcgccucaa gaaguacgcc     1920

aaccuguucg acgacaaggu caugaagcag cugaagcggc gccacuacac cggcuggggg     1980

cggcucuccc gcaagcugau caacggcauc cgggacaagc agagcgggaa gacgauccug     2040

gacuuccuca aguccgacgg cuucgccaac cgcaacuuca ugcagcugau caacgacgac     2100

agccugaccu ucaaggaggc cauccagaag gcccaggugu ccggccaggg gcacagccuc     2160

cacgagcaga ucgcgaaccu ggccggcagc cccgccauca agaaggggau ccugcagucc     2220

gucaaggugg uggacgagcu cgucaaggug augggccaca agcccgagaa caucgugauc     2280

gagauggccc gcgagaacca gaccacccag aaggggcaga agaacagccg ggagcgcaug     2340

aagcggaucg aggagggcau caaggagcug ggcucccaga uccugaagga gcaccccguc     2400

gagaacacgc agcuccagaa cgagaagcug uaccuguacu accuccagaa cgggcgcgac     2460

auguacgugg accaggagcu ggacaucaac cggcugagcg acuacgacgu ggaccacauc     2520

gucccgcagu ccuucaucaa ggacgacagc aucgacaaca agauccucac ccgcuccgac     2580

aagaaccggg gcaagagcga caacgugccc uccgaggagg uggucaagaa gaugaagaac     2640

uacuggcgcc agcugcugaa cgccaagcuc aucacccagc ggaaguucga caaccugacc     2700

aaggcggagc gcgggggccu gagcgagcuc gacaaggccg gcuucaucaa gcggcagcug     2760

guggagacgc gccagaucac caagcacgug gcccagaucc uggacucccg gaugaacacc     2820

aaguacgacg agaacgacaa gcucauccgc gaggucaagg ugaucacccu gaaguccaag     2880

cugguguccg acuuccggaa ggacuuccag uucuacaagg uccgcgagau caacaacuac     2940

caccacgccc acgacgccua ccucaacgcg guggugggga ccgcccugau caagaaguac     3000

acgaagcugg agagcgaguu cgucuacggc gacuacaagg uguacgacgu gcggaagaug     3060

aucgccaagu ccgagcagga gaucgggaag gccaccgcga agcgcuucuu cuacagcaac     3120

aucaugaacu ucuucaagac cgagaucacc cucgccaacg gcgagauccg gaagcgcccc     3180

cugaucgaga cgaacgagga gaccggggag aucgucuggg acaagggccg ggacuucgcc     3240

accgugcgca aggugcuguc caugccccag gucaacaucg ugaagaagac cgaggugcag     3300

acgggcgccc ucaccaacga gagcaucuac gcccgcggga gcuucgacaa gcugaucucc     3360

cggaagcacc gcuucgagag cuccaaguac ggcggguucg gcagcccgac cgucaccuac     3420

uccgugcugg uggucgccaa gagcaaggug caggacggga aggugaagaa gaucaagacg     3480

ggcaaggagc ucaucggcau gacccugcug gacaagcucg ucuucgagaa gaacccccug     3540

aaguucaucg aggacaaggg guacggcaac gugcagaucg acaagugcau caagcugccc     3600

aaguacuccc ucuucgaguu cgagaacggg acccggcgca ugcuggccag cgugauggcc     3660

aacaacaacu cccggggcga ccugcagaag gccaacgaga uguuccuccc cgccaagcug     3720

gucacccugc ucuaccacgc gcacaagauc gagagcucca aggagcugga gcacgaggcc     3780

uacauccugg accacuacaa cgaccucuac cagcugcuga gcuacaucga gcgcuucgcc     3840

ucccucuacg uggacgugga gaagaacauc agcaagguca aggagcuguu cuccaacauc     3900

gagagcuacu ccaucagcga gaucugcucc agcgugauca accugcucac gcugaccgcc     3960

agcggggccc cggcggacuu caaguuccug ggcaccacca ucccccggaa gcgcuacggc     4020

agcccccaga gcauccucuc cagcacgcug auccaccagu ccaucaccgg gcuguacgag     4080

acccggaucg accucagcca gcugggcggg gacuga                               4116


<210>  1991
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1991
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcauccgg      240

uaccugcagg agaucuucuc cagcgagaug uccaaggucg acgacagcuu cuuccaccgc      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcggcaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccgcaag      420

aagcucgccg acagcaccga caaggcggac cugcggcuga ucuaccucgc ccuggcccac      480

augaucaagu uccgcggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

guggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgcca gccgggucga cgcgaaggcc auccuguccg cccgccugag caagucccgg      660

cgccucgaga accugaucgc ccagcugccg ggcgagaagc ggaacggccu cuucgggaac      720

cugaucgccc ugagccucgg gcugacgccc aacuucaagu ccaacuucga ccuggcggag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgccgcga agaaccuguc cgacgccauc      900

cugcucagcg acauccugcg cgugaacagc gagaucacca aggccccgcu guccgccagc      960

augaucaagc gguacgacga gcaccaccag gaccucaccc ugcugaaggc ccucgugcgc     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc aguccaagaa cggcuacgcg     1080

ggguacaucg acggcggggc cagccaggag gaguucuaca aguucaucaa gccgauccug     1140

gagaagaugg acggcacgga ggagcuccug gccaagcuga accgcgagga ccugcuccgg     1200

aagcagcgca ccuucgacaa cggcuccauc ccccaccaga uccaccuggg ggagcugcac     1260

gccauccucc ggcgccagga ggacuucuac cccuuccuga aggacaaccg ggagaagauc     1320

gagaagaucc ugaccuuccg cauccccuac uacgucggcc cgcucgcccg ggggaacagc     1380

cgcuucgcgu ggaugacccg gaaguccgag gagacgauca cccccuggaa cuucgaggag     1440

gugguggaca agggcgccag cgcccagucc uucaucgagc gcaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu ccugcccaag cacagccucc uguacgagua cuucaccgug     1560

uacaacgagc ugacgaaggu gaaguacguc accgagggga ugcggaagcc ggccuuccuc     1620

uccggcgagc agaagaaggc gaucguggac cugcuguuca agaccaaccg caaggugacc     1680

gucaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacag cguggagauc     1740

uccggcgugg aggaccgguu caacgccagc cuggggacgu accacgaccu gcucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucguc     1860

cucacccuga cccuguucga ggacaaggag augaucgagg agcgccucaa gaccuacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc gccacuacac gggcuggggg     1980

cggcucuccc gcaagcugau caacggcauc cgggacaagc agagcgggaa gaccauccug     2040

gacuuccuca aguccgacgg cuucgccaac cgcaacuuca uccagcugau ccacgacgac     2100

agccugaccu ucaaggaggc cauccagaag gcgcaggucu ccggccaggg gcacagccuc     2160

cacgagcaga ucgccaaccu ggccggcagc cccgccauca agaaggggau ccugcagucc     2220

gugaaggugg ucgacgagcu cgugaaggug augggccaca agcccgagaa caucgucauc     2280

gagauggccc gcgagaacca gaccacgcag aaggggcaga agaacagccg ggagcgcaug     2340

aagcggaucg aggagggcau caaggagcug ggcucccaga uccugaagga gcaccccgug     2400

gagaacaccc agcuccagaa cgagaagcug uaccuguacu accuccagaa cgggcgcgac     2460

auguacgugg accaggagcu ggacaucaac cggcugagcg acuacgacgu cgaccacauc     2520

gugccgcagu ccuucaucaa ggacgacagc aucgacaaca aggugcucac ccgcuccgac     2580

aagaaccggg gcaagagcga caacgucccc uccgaggagg uggugaagaa gaugaagaac     2640

uacuggcgcc agcugcugaa cgcgaagcuc aucacccagc ggaaguucga caaccugacg     2700

aaggccgagc gcgggggccu gagcgagcuc gacaaggccg gcuucaucaa gcggcagcug     2760

gucgagaccc gccagaucac caagcacgug gcccagaucc uggacucccg gaugaacacc     2820

aaguacgacg agaacgacaa gcucauccgc gaggugaagg ucaucacccu caagagcaag     2880

cugguguccg acuuccggaa ggacuuccag uucuacaagg ugcgcgagau caacaacuac     2940

caccacgccc acgacgcgua ccucaacgcc gucgugggga cggcccugau caagaaguac     3000

cccaagcugg agagcgaguu cguguacggc gacuacaagg ucuacgacgu gcggaagaug     3060

aucgccaagu ccgagcagga gaucgggaag gcgaccgcca agcgcuucuu cuacagcaac     3120

aucaugaacu ucuucaagac cgagaucacc cucgccaacg gcgagauccg gaagcgcccc     3180

cugaucgaga cgaacgagga gaccggggag aucguguggg acaagggccg ggacuucgcc     3240

accguccgca aggugcuguc caugccccag gugaacaucg ucaagaagac cgaggugcag     3300

acgggcgccc ucaccaacga gagcaucuac gcgcgcggga gcuucgacaa gcugaucucc     3360

cggaagcacc gcuucgagag cuccaaguac ggcggguucg gcagccccac cgugaccuac     3420

uccguccugg ugguggccaa gagcaagguc caggacggga aggugaagaa gaucaagacg     3480

ggcaaggagc ucaucggcau cacccugcug gacaagcucg uguucgagaa gaacccccug     3540

aaguucaucg aggacaaggg guacggcaac guccagaucg acaagugcau caagcugccc     3600

aaguacuccc ucuucgaguu cgagaacggg acccggcgca ugcuggccag cgugauggcc     3660

aacaacaacu cccggggcga ccugcagaag gccaacgaga uguuccuccc ggcgaagcug     3720

gugacccugc ucuaccacgc ccacaagauc gagagcucca aggagcugga gcacgaggcc     3780

uacauccugg accacuacaa cgaccucuac cagcugcuga gcuacaucga gcgcuucgcc     3840

ucccucuacg ucgacgugga gaagaacauc agcaagguga aggagcuguu cuccaacauc     3900

gagagcuacu ccaucagcga gaucugcucc agcgucauca accugcucac gcugaccgcc     3960

agcggggcgc ccgccgacuu caaguuccug ggcaccacca ucccccggaa gcgcuacggc     4020

uccccgcaga gcauccucuc cagcacgcug auccaccagu ccaucaccgg gcuguacgag     4080

acccggaucg accucagcca gcugggcggg gacuga                               4116


<210>  1992
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1992
auggagaaca agaacuacag caucggccug gacaucggga ccaacuccgu gggcuggagc       60

gucaucaccg acgacuacaa ggugcccucc aagaagauga aggugcucgg gaacacggac      120

aagcacuuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcc      180

gaggcgcgcc ggcugaagcg caccgcccgg cgccgguaca cgcgccggaa gaaccgccug      240

cgguaccucc aggagaucuu cagcgaggag aucggcaagg ucgacuccag cuucuuccac      300

cgccuggacg acuccuuccu gaucccggag gacaagcggg gcagcaagua ccccaucuuc      360

gccacccucg ccgaggagaa gaaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacuccaa ggagaagacc gaccuccggc ugaucuaccu ggcgcucgcc      480

cacaugauca aguaccgcgg gcacuuccug uacgaggagg ccuucgacau caagaacaac      540

gacauccaga agaucuucaa cgaguucauc agcaucuaca acaacacguu cgagggcaac      600

ucccugagcg ggcagaacgu ccagguggag gccaucuuca ccgacaagau cuccaagagc      660

gcgaagcggg agcgcgugcu caagcuguuc cccgacgaga aguccaccgg ccuguucagc      720

gaguuccuca agcugaucgu ggggaaccag gccgacuuca agaagcacuu cgaccuggag      780

gagaaggccc cgcuccaguu cucccgggac accuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gcgacgacuu cgccgaccug uuccuggugg ccaagaagcu cuacgacgcg      900

auccugcuga gcgggauccu cacggucacc aaccccucca ccaaggcccc gcugagcgcc      960

agcaugaucg agcgcuacga gaaccaccag aaggaccugg ccucccucaa gcaguucauc     1020

aagaacaacc ugcccgagaa guacgacgag guguucagcg accaguccga ggacggcuac     1080

gccggguaca ucgacggcaa gaccacgcag gagaccuucu acaaguacau caagaaccug     1140

cucagcaagu ucgagggcgc ggacuacuuc cuggacaaga ucgagcggga ggacuuccug     1200

cgcaagcagc ggaccuucga caacgggucc aucccgcacc agauccaccu ccaggagaug     1260

aacgccaucc ugcgccgcca gggcgagcac uaccccuucc ugaaggagaa caaggagaag     1320

aucgagaaga uccucaccuu ccggaucccc uacuacgugg ggccccuggc ccgcggcaac     1380

cgggacuucg ccuggcugac gcgcaacagc gaccaggcca uccggccgug gaacuucgag     1440

gagaucgucg acaaggcguc cagcgccgag gacuucauca acaagaugac caacuacgac     1500

cucuaccugc ccgaggagaa ggugcugccc aagcacuccc ugcucuacga gaccuucgcc     1560

guguacaacg agcugaccaa ggucaaguuc aucgccgagg ggcugcgcga cuaccaguuc     1620

cucgacagcg gccagaagaa gcagaucgug acgcagcugu ucaaggagaa gcggaaggug     1680

accgagaagg acaucaucca guuccugcac aacgucgacg gcuacgacgg gaucgagcuc     1740

aagggcaucg agaagcaguu caacgccucc cugagcaccu accacgaccu gcucaagauc     1800

aucaaggaca aggcguucau ggacgacgcc aagaacgagg ccauccugga gaacaucgug     1860

cacacccuga cgaucuucga ggaccgcgag augaucaagc agcggcucgc ccaguacgac     1920

ucccuguucg acgagaaggu gaucaaggcg cugacccgcc ggcacuacac cggguggggc     1980

aagcucagcg ccaagcugau caacgggauc uccgacaagc agaccggcaa cacgauccug     2040

gacuaccuca ucgacgacgg cgagaucaac cgcaacuuca ugcagcugau caacgacgac     2100

gggcugagcu ucaaggagau cauccagaag gcccaggucg ugggcaagac caacgacgug     2160

aagcaggucg ugcaggagcu ccccgggucc ccggccauca agaagggcau ccugcagagc     2220

aucaagaucg ucgacgagcu ggugaaggug auggggcacg cccccgaguc caucgucauc     2280

gagauggcgc gggagaacca gaccaccgcc cgcggcaaga agaacagcca gcagcgguac     2340

aagcgcaucg aggacagccu caagauccug gccuccggcc ugaacagcaa gauccucaag     2400

gagcacccca cggacaacau ccagcugcag aacgaccggc uguuccucua cuaccugcag     2460

aacgggaagg acauguacac cggcgaggcc cuggacauca accagcucuc cagcugcgac     2520

aucgaccaca ucauccccca ggccuucauc aaggacgacu cccuggacaa ccgcgugcug     2580

accagcucca aggacaaccg gggcaagagc gacaacgugc cgucccucga ggucguggac     2640

aagaugaagg uguucuggca gcagcugcug gacagcaagc ucaucuccua ccgcaaguuc     2700

aacaaccuga ccaaggcgga gcgcgggggc cugaacgagc ucgacaaggu cggguucauc     2760

aagcggcagc ugguggagac ccgccagauc acgaagcacg uggcccagau ccuggacgcc     2820

cgguucaaca aggaggucac cgagaaggac aagaagaacc gcaccgugaa gaucaucacc     2880

cucaagagca accugguguc caacuuccgg aaggaguucg agcuguacaa gguccgcgag     2940

aucaacgacu accaccacgc ccacgacgcc uaccucaacg cggugguggc caaggccauc     3000

cugaagaagu accccaagcu ggagcccgag uucgucuacg gcgacuacca gaaguacgac     3060

cucaagcggu acaucagccg cuccaaggac cccaaggaga ucgagaaggc cacggagaag     3120

uacuucuucu acagcaaccu gcugaacuuc uucaaggagg aggugcacua cgcggacggg     3180

accaucguga agcgggagaa caucgaguac uccaaggaca ccggcgagau cgccuggaac     3240

aaggagaagg acuucgccac caucaagaag guccucagcc ugccccaggu gaacaucgug     3300

aagaagcgcg agguccagac cgggggcuuc agcaaggagu ccauccugcc caagggcaac     3360

agcgacaagc ucaucccccg gaagacgaag gacauccugu gggacaccac caaguacggg     3420

ggcuucgacu cccccgugau cgccuacagc auccugcuca ucgccgacau cgagaagggg     3480

aaggcgaaga agcugaagac ggugaagacc cuggucggca ucaccaucau ggagaaggac     3540

gccuucgaga agaacccgau cgccuuccuc gagaacaagg gguaccacaa cgugugcaag     3600

gagaacaucc ugugccugcc caaguacucc cucuucgagc uggagaacgg ccgccggcgc     3660

cugcucgcca gcgccaagga gcugcagaag ugcaacgaga ucgugcugcc cgucuaccuc     3720

accacgcugc uguaccacuc caagaacgug cacaagcucg acgagccggg ccaccuggag     3780

uacauccaga agcaccggaa cgaguucaag gaccugcuca accuggugag cgaguucucc     3840

cagaaguacg uccuggcgga cgccaaccuc gagaagauca agaaccugua cgccgacaac     3900

gagcaggccg acaucgagau ccuggccaac agcuucauca accuccugac cuucaccgcg     3960

cugggggccc ccgccgccuu caaguucuuc ggcaaggacg uggaccgcaa gcgguacacc     4020

acgguguccg agauccucaa cgcgacccug auccaccaga gcaucaccgg gcuguacgag     4080

acccgcaucg accucuccaa gcugggcgag gacuga                               4116


<210>  1993
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1993
augaagguga acaacuacca caucggccug gacaucggga ccagcuccau cggcuggguc       60

gccaucgggg aggacggcaa gccccuccgc aucaagggga agaccgccau cggcgcgcgg      120

cuguuccagg agggcaaccc ggccgccgac cgccggaugu uccgcacgac ccggcgccgg      180

cugagccgcc ggaaguggcg ccucaagcug cuggaggaga ucuucgaccc cuacaucacc      240

cccguggacu ccaccuucuu cgcccggcuc aagcagagca accugucccc caaggacagc      300

cgcaaggagu ucaagggguc caugcuguuc ccggaccuca cggacaugca guaccacaag      360

gacuacccca ccaucuacca ccuccggcac gcccugauga cccaggacga gaaguucgac      420

auccgcaugg uguaccuggc gauccaccac aucgucaagu accggggcaa cuuccugaac      480

agcacccccg uggacuccuu caaggccagc aaggugaacu ucgucgacca guucaagaag      540

cugaacgagc ucuacacggc caucaacccc gaggaguccu uccagaucaa ccuggccaac      600

agcgaggaca ucgggcacca guuccuggac ccguccaucc gcaaguucga caagaagaag      660

cagaucccca agaucgugcc cgugagcguc gacgacaagg cgaccgacaa gaucaacggc      720

aagaucgccu ccgagaucau caacgccauc cucggguaca agagcaagcu ggacguggug      780

guccagugca cccccgugga cuccaagagc ugggcccuga aguucgacga ggaggacauc      840

gacgccaagc uccagaagau ccugccggag auggacgaga accagcaguc caucaucgcg      900

auccugcaga accucuacag ccaggugacc cugaaccaga ucguccccaa cggcaugagc      960

cuguccgaga gcaugaucga gaaguacaac gaccaccacg accaccucaa gcuguacaag     1020

aagaucaucg accagcuggc cgaccccaag aagaaggccg cccucaagaa ggccuacucc     1080

caguacgugg gcgacgacgg gaaggugauc gagcaggcgg acuucuggag cuccgucaag     1140

aagaaccugg acgacagcga ccuguccaag cagaucaugg accucaucga cgccgagaag     1200

uucaugccca agcagcggac gagccagaac ggcgugaucc cgcaccagcu gcaccagcgc     1260

gagcuggacg agaucaucga gcaccagucc aaguacuacc ccuggcucgc cgagaucaac     1320

cccaacaagc acgaccugca ccuggccaag uacaagaucg aggagcucgu ggccuuccgg     1380

guccccuacu acguggggcc gaugaucacc cccgacgacc aggccaagag cgcggagacc     1440

guguucuccu ggauggagcg caagggcaag gaggccggcc agaucacccc cuggaacuuc     1500

gacgagaagg ucgaccggaa cgccagcgcc aaccgcuuca ucaagcgcau gacgaccaag     1560

gacaccuacc ugaucgggga ggacgugcug cccgacgagu cccuccugua cgagaaguuc     1620

aagguccuga acgagcucaa cauggugcgg gugaacggca agcugcugaa ggucgcggac     1680

aagcaggcca ucuuccagga ccucuucgag aacuacaagc acaucagcgu gaagaagcug     1740

cagaacuaca ucaaguccaa gaccgggcug ccgagcgacc ccgagaucuc cggccugagc     1800

gaccccgagu acuucaacaa cucccucggg acguacaacg acuucaagaa gcuguucggc     1860

aacaaggugg acgagcccga ccugcaggac gacuucgaga agaucgucga guggagcacc     1920

guguucgagg acaagcgcau ccuccgggag aagcugaacg agaucaccug gcugagcgac     1980

cagcagaagg acgugcucga guccagccgc uaccagggcu gggggcggcu guccaagaag     2040

cugcucaccg gcaucgucaa cgaccagggg gagcgcauca ucgacgagcu guggaacacg     2100

aacaagaacu ucaugcagau ccagagcgac aacgacuucg ccaagcggau ccacgaggcc     2160

aacgcggacc agaugaaggc cguggacgug gaggacgucc uggccgacgc cuacaccucc     2220

ccgcagaaca agaaggccau ccgccaggug gugaaggucg uggacgacau ccagaaggcg     2280

augggcgggg uggcccccaa guacaucagc aucgaguuca cccgguccga ggaccgcaac     2340

ccccggcgca ccaucagccg gcagcgccag cucgagaaca cgcugaagga caccgccaag     2400

ucccuggcca agagcaucaa cccggagcuc cuguccgagc uggacaacgc cgccaagagc     2460

aagaagggcc ucaucgaccg gcuguaccug uacuucaccc agcucggcaa ggacaucuac     2520

accgggaagc ccaucaacau cgacgagauc uccacguacg acaucgacca cauccugccc     2580

caggcguucg ucaaggacga cagccugaac aaccgcgugc ucguguccaa ggccaucaac     2640

aacggcaaga gcgacaacgu ccccgugcag cuguucgggg ccaagauggg ccacuucugg     2700

aagcagcugg ccgaggcggg gcucaucucc aagcggaagc ugaagaaccu gcagaccgac     2760

ccggacacca ucagcaagua cgccaugcac ggcuucaucc gccggcagcu cguggagacc     2820

agccagguca ucaagcuggu ggccaacauc cugggcgaca aguaccgcaa cgacgacacg     2880

aagaucaucg agaucaccgc ccgcaugaac caccagaugc gggacgaguu cggguucauc     2940

aagaaccgcg agaucaacga cuaccaccac gcguucgacg ccuaccucac cgccuuccug     3000

ggccgguacc uguaccaccg cuacaucaag cuccggcccu acuucgugua cggggacuuc     3060

aagaaguuca aggaggacaa ggucaccaug cgcaacuuca acuuccugca cgaccugacc     3120

gacgacacgc aggagaagau cgccgacgcc gagaccggcg aggugaucug ggaccgggag     3180

aacuccaucc agcagcucaa ggacguguac cacuacaagu ucaugcugau cagccacgag     3240

gucuacaccc ugcgcggcgc gauguucaac cagaccgugu accccgccuc cgacgccggg     3300

aagcggaagc ucauccccau caaggccgac cgcccgauca acguguacgg cggguacagc     3360

ggcuccgccg acgcguacau ggccaucguc cggauccaca acaagaaggg ggacaaguac     3420

cgcguggugg gcguccccau gcgggcccgc gaccggcugg acgccgccaa gaaggugagc     3480

gacgcggacu acgaccgcgc ccugaaggac gugcucacgc cccagcugac caagaccaag     3540

aagucccgga agaccggcga gaucacgcag gucguggagg acuucgagau cgugcugggg     3600

aaggucaugu accgccagcu caugaucgac ggcgacaaga aguucaugcu ggggagcucc     3660

accuaccagu acaacgccaa gcagcuggug cucagcgacc aguccgugaa gacccuggcc     3720

agcaagggcc ggcuggaccc ccuccaggag uccauggacu acaacaacgu cuacaccgag     3780

auccuggaca aggugaacca guacuucagc cuguacgaca ugaacaaguu ccgccacaag     3840

cucaaccugg gguucuccaa guucaucagc uucccgaacc acaacguguu cgacggcaac     3900

acgaaggcgu ccagcggcaa gcgggagauc cuggaggagg uccucaacgg gcugcacgcc     3960

aaccccaccu ucggcaaccu gaaggacauc gggaucacca cccccuucgg ccagcuccag     4020

cagcccaacg ggauccugcu guccgacgag gccaagaucc gcuaccagag cccgacgggc     4080

cucuucgagc gcaccgugag ccugaaggac cuguga                               4116


<210>  1994
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1994
augaagguga acaacuacca caucggccug gacaucggga ccagcuccau cggcuggguc       60

gccaucggga aggacggcaa gccccuccgc gugaagggga agaccgccau cggcgcgcgg      120

cuguuccagg agggcaaccc ggccgccgac cgccggaugu uccgcacgac ccggcgccgg      180

cugagccgcc ggaaguggcg ccucaagcug cuggaggaga ucuucgaccc cuacaucacc      240

cccguggacu ccaccuucuu cgcccggcuc aagcagagca accugucccc caaggacagc      300

cgcaaggagu ucaagggguc caugcuguuc ccggaccuca cggacaugca guaccacaag      360

aacuacccca ccaucuacca ccuccggcac gcccugauga cccaggacaa gaaguucgac      420

auccgcaugg ucuaccuggc gauccaccac aucgugaagu accggggcaa cuuccugaac      480

agcacccccg uggacuccuu caaggccagc aaggucgacu ucguggacca guucaagaag      540

cugaacgagc ucuacgccgc caucaacccc gaggaguccu ucaagaucaa ccuggcgaac      600

agcgaggaca ucgggcacca guuccuggac ccguccaucc gcaaguucga caagaagaag      660

cagaucccca agaucgugcc cgucaugaug aacgacaagg ugacggaccg gcucaacggc      720

aagaucgcca gcgagaucau ccacgccauc cugggguaca aggccaagcu ggacgugguc      780

cuccagugca cccccgugga cuccaagccg ugggcccuga aguucgacga cgaggacauc      840

gacgcgaagc uggagaagau ccuccccgag auggacgaga accagcagag caucguggcc      900

auccugcaga accuguacuc ccaggucacc cucaaccaga ucgugcccaa cggcaugagc      960

cuguccgaga gcaugaucga gaaguacaac gaccaccacg accaccugaa gcucuacaag     1020

aagcugaucg accagcuggc cgaccccaag aagaaggccg ugcucaagaa ggccuacagc     1080

caguacgucg gcgacgacgg gaaggugauc gagcaggcgg aguucugguc cagcgugaag     1140

aagaaccugg acgacuccga gcugagcaag cagaucaugg accucaucga cgccgagaag     1200

uucaugccga agcagcgcac cucccagaac ggcgucaucc cccaccagcu gcaccagcgg     1260

gagcuggacg agaucaucga gcaccagagc aaguacuacc ccuggcucgu ggagaucaac     1320

cccaacaagc acgaccugca ccuggccaag uacaagaucg agcagcucgu ggccuuccgc     1380

gucccguacu acguggggcc caugaucacg cccaaggacc aggccgaguc cgcggagacc     1440

gucuucagcu ggauggagcg gaagggcacc gagaccggcc agaucacgcc cuggaacuuc     1500

gacgagaagg uggaccgcaa ggccuccgcc aaccgcuuca ucaagcggau gaccaccaag     1560

gacaccuacc ugaucgggga ggacgugcug ccggacgaga gccuccugua cgagaaguuc     1620

aagguccuga acgagcucaa cauggugcgc gugaacggca agcugcugaa ggucgccgac     1680

aagcaggcca ucuuccagga ccuguucgag aacuacaagc acguguccgu gaagaagcuc     1740

cagaacuaca ucaaggcgaa gacggggcug cccagcgacc ccgagaucuc cggccugagc     1800

gaccccgagc acuucaacaa cucccucggg accuacaacg acuucaagaa gcuguucggc     1860

agcaaggucg acgagccgga ccugcaggac gacuucgaga agaucgugga gugguccacc     1920

guguucgagg acaagaagau ccuccgggag aagcugaacg agaucaccug gcugagcgac     1980

cagcagaagg acguccucga guccagccgc uaccagggcu gggggcggcu gagcaagaag     2040

cugcucacgg gcaucgugaa cgaccagggg gagcgcauca ucgacaagcu guggaacacc     2100

aacaagaacu ucaugcagau ccaguccgac gacgacuucg ccaagcggau ccacgaggcc     2160

aacgccgacc agaugcaggc gguggacguc gaggacgugc uggccgacgc cuacaccagc     2220

ccccagaaca agaaggccau ccgccaggug gucaaggugg uggacgacau ccagaaggcc     2280

augggcgggg ucgcgcccaa guacaucucc aucgaguuca cccggagcga ggaccgcaac     2340

ccgcggcgca cgaucucccg gcagcgccag cucgagaaca cccugaagga caccgccaag     2400

agccuggcca aguccaucaa ccccgagcuc cugagcgagc uggacaacgc cgccaagucc     2460

aagaagggcc ucaccgaccg gcuguaccug uacuucacgc agcucggcaa ggacaucuac     2520

accggggagc ccaucaacau cgacgagcug aacaaguacg acaucgacca cauccugccc     2580

caggcguuca ucaaggacaa cagccucgac aaccgcgugc uggugcugac cgccgucaac     2640

aacggcaagu ccgacaacgu gccgcuccgg auguucgggg ccaagauggg ccacuucugg     2700

aagcagcugg ccgaggccgg gcugaucagc aagcgcaagc ucaagaaccu gcagaccgac     2760

cccgacacga ucuccaagua cgcgaugcac ggcuucaucc ggcgccagcu gguggagacc     2820

agccagguca ucaagcucgu ggccaacauc cugggcgaca aguaccgcaa cgacgacacc     2880

aagaucaucg agaucaccgc ccggaugaac caccagaugc gcgacgaguu cggguucauc     2940

aagaaccggg agaucaacga cuaccaccac gccuucgacg cguaccugac cgccuuccuc     3000

ggccgcuacc uguaccaccg guacaucaag cugcgccccu acuucgugua cggggacuuc     3060

aagaaguucc gggaggacaa ggucacgaug cgcaacuuca acuuccucca cgaccugacc     3120

gacgacaccc aggagaagau cgccgacgcc gagaccggcg aggugaucug ggaccgggag     3180

aacuccaucc agcagcugaa ggacguguac cacuacaagu ucaugcucau cagccacgag     3240

gucuacacgc ugcgcggcgc cauguucaac cagaccgugu accccgcguc cgacgccggg     3300

aagcggaagc ugaucccggu gaaggccgac cgccccguca acguguacgg cggguacagc     3360

ggcagcgccg acgccuacau ggcgaucgug cggauccaca acaagaaggg ggacaaguac     3420

cgcgucgugg gcgugcccau gcgggcccuc gaccgccugg acgccgccaa gaacgucucc     3480

gacgccgacu ucgaccgggc gcugaaggac gugcucgcgc cccagcugac caagaccaag     3540

aagucccgca agacgggcga gaucacccag gugaucgagg acuucgagau cguccugggg     3600

aaggugaugu accggcagcu caugaucgac ggcgacaaga aguucaugcu ggggagcagc     3660

accuaccagu acaacgccaa gcagcuggug cucuccgacc agagcgucaa gacccuggcc     3720

uccaagggcc gccuggaccc gcuccaggag agcauggacu acaacaacgu guacacggag     3780

auccuggaca aggugaacca guacuucucc cuguacgaca ugaacaaguu ccggcacaag     3840

cucaaccugg gguucagcaa guucaucucc uuccccaacc acaacguccu ggacggcaac     3900

accaagguga gcuccggcaa gcgcgagauc cuccaggaga uccugaacgg gcugcacgcc     3960

aaccccaccu ucggcaaccu caaggacguc gggaucacca cgcccuucgg ccagcugcag     4020

cagccgaacg ggauccugcu cagcgacgag accaagaucc gcuaccaguc ccccaccggc     4080

cuguucgagc ggaccgugag ccugaaggac cucuga                               4116


<210>  1995
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1995
auggacaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacggaccgc      120

cacuccauca agaagaaccu gaucggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcaucugc      240

uaccugcagg agaucuucuc caacgagaug gccaaggucg acgacagcuu cuuccaccgg      300

cucgaggagu ccuuccuggu ggaggaggac aagaagcacg agcgccaccc gaucuucggc      360

aacaucgugg acgaggucgc cuaccacgag aaguacccca ccaucuacca ccuccggaag      420

aagcucgcgg acagcaccga caaggccgac cugcgccuga ucuaccucgc ccuggcccac      480

augaucaagu uccggggcca cuuccugauc gagggggacc ucaaccccga caacuccgac      540

guggacaagc uguucaucca gcuggugcag accuacaacc agcuguucga ggagaacccc      600

aucaacgcga gccgcgucga cgccaaggcc auccuguccg cccggcugag caagucccgc      660

cggcucgaga accugaucgc ccagcugccg ggcgagaaga agaacggccu cuucgggaac      720

cugaucgcgc ugagccucgg gcugacgccc aacuucaagu ccaacuucga ccuggccgag      780

gacgccaagc uccagcugag caaggacacc uacgacgacg accuggacaa ccuccuggcc      840

cagaucggcg accaguacgc cgaccuguuc cucgcggcca agaaccuguc cgacgccacc      900

cugcucagcg acauccugcg cgugaacucc gagaucacca aggccccgcu gagcgccagc      960

augaucaagc gguacgacga gcaccaccag gaccucacgc ugcugaaggc gcucgugcgc     1020

cagcagcugc ccgagaagua caaggagauc uucuucgacc aguccaagaa cggcuacgcc     1080

ggguacaucg acggcggggc cagccaggag gaguucuaca aguucaucaa gccgauccug     1140

gagaagaugg acggcaccga ggagcuccug gccaagcuga accgggagga ccugcuccgc     1200

aagcagcgca ccuucgacaa cggcuccauc ccccaccaga uccaccuggg ggagcugcac     1260

gccauccucc ggcgccagga ggacuucuac cccuuccuga aggacaaccg ggagaagauc     1320

gagaagaucc ugaccuuccg cauccccuac uacgucggcc cgcucgcgcg ggggaacagc     1380

cgcuucgccu ggaugacgcg gaaguccgag gagaccauca cccccuggaa cuucgaggag     1440

gugguggaca agggcgccag cgcccagucc uucaucgagc gcaugaccaa cuucgacaag     1500

aaccugccca acgagaaggu ccugcccaag cacagccucc uguacgagua cuucacggug     1560

uacaacgagc ugaccaaggu gaaguacguc accgagggga ugcggaagcc ggcguuccuc     1620

uccggcgagc agaagaaggc caucguggac cugcuguuca agaccaaccg caaggugacg     1680

gucaagcagc ucaaggagga cuacuucaag aagaucgagu gcuucgacag cguggagauc     1740

uccggcgugg aggaccgguu caacgccagc cuggggaccu accacgaccu gcucaagauc     1800

aucaaggaca aggacuuccu ggacaacgag gagaacgagg acauccugga ggacaucguc     1860

cucacccuga cccuguucga ggacaaggag augaucgagg agcgccucaa gacguacgcc     1920

caccuguucg acgacaaggu gaugaagcag cugaagcggc gccacuacac cggcuggggg     1980

cggcucuccc gcaagcugau caacggcauc cgggacaagc agagcgggaa gaccauccug     2040

gacuuccuca aguccgacgg cuucgccaac cgcaacuuca uccagcugau ccacgacgac     2100

agccugaccu ucaaggaggc gauccagaag gcccaggucu ccggccaggg gcacagccuc     2160

cacgagcaga ucgccaaccu ggccggcucc cccgccauca agaaggggau ccugcagagc     2220

gugaaggugg ucgacgagcu cgugaaggug augggccaca agcccgagaa caucgucauc     2280

gagauggcgc gggagaacca gacgacccag aaggggcaga agaacagccg cgagcgcaug     2340

aagcggaucg aggagggcau caaggagcug ggcucccaga uccugaagga gcaccccgug     2400

gagaacaccc agcuccagaa cgagaagcug uaccuguacu accuccagaa cgggcgcgac     2460

auguacgugg accaggagcu ggacaucaac cggcugagcg acuacgacgu cgaccacauc     2520

gugccgcagu ccuucaucaa ggacgacagc aucgacaaca aggugcucac ccgcuccgac     2580

aagaaccggg gcaagagcga cgacgucccc uccgaggagg uggugaagaa gaugaagaac     2640

uacuggcgcc agcugcugaa cgccaagcuc aucacgcagc ggaaguucga caaccugacc     2700

aaggccgagc gcgggggccu gagcgagcuc gacaaggccg gcuucaucaa gcggcagcug     2760

gucgagaccc gccagaucac caagcacgug gcccagaucc uggacucccg gaugaacacc     2820

aaguacgacg agaacgacaa gcucauccgc gaggugaagg ucaucacgcu caagagcaag     2880

cugguguccg acuuccggaa ggacuuccag uucuacaagg ugcgcgagau caacaacuac     2940

caccacgcgc acgacgccua ccucaacgcc gucgugggga ccgcccugau caagaaguac     3000

cccaagcugg agagcgaguu cguguacggc gacuacaagg ucuacgacgu gcggaagaug     3060

aucgcgaagu ccgagcagga gaucgggaag gccaccgcca agcgcuucuu cuacagcaac     3120

aucaugaacu ucuucaagac cgagaucacg cucgccaacg gcgagauccg gaagcgcccc     3180

cugaucgaga ccaacgagga gaccggggag aucguguggg acaagggccg ggacuucgcc     3240

accguccgca aggugcuguc caugccccag gugaacaucg ucaagaagac ggaggugcag     3300

accggcgcgc ucaccaacga gagcaucuac gcccgggggu ccuucgacaa gcugaucagc     3360

cgcaagcacc gcuucgagag cuccaaguac ggcggguucg gcagccccac cgugacguac     3420

uccguccugg ugguggccaa gagcaagguc caggacggga aggugaagaa gaucaagacc     3480

ggcaaggagc ucaucggcau cacccugcug gacaagcucg uguucgagaa gaacccccug     3540

aaguucaucg aggacaaggg guacggcaac guccagaucg acaagugcau caagcugccc     3600

aaguacuccc ucuucgaguu cgagaacggg acccggcgca ugcuggccag cgugauggcc     3660

aacaacaacu cccggggcga ccugcagaag gcgaacgaga uguuccuccc ggccaagcug     3720

gugacgcugc ucuaccacgc ccacaagauc gagagcucca aggagcugga gcacgaggcc     3780

uacauccugg accacuacaa cgaccucuac cagcugcuga gcuacaucga gcgcuucgcc     3840

ucccucuacg ucgacgugga gaagaacauc agcaagguga aggagcuguu cuccaacauc     3900

gagagcuacu ccaucagcga gaucugcucc agcgucauca accugcucac ccugaccgcg     3960

uccggggccc ccgccgacuu caaguuccug ggcaccacga ucccccggaa gcgcuacggc     4020

agcccgcaga gcauccucuc cagcacccug auccaccagu ccaucaccgg gcuguacgag     4080

acccggaucg accucagcca gcugggcucc gacuga                               4116


<210>  1996
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1996
augaacaacg aguacuucgu gggcuucgac aucgggacca acagcgucgg cgccgccgug       60

accgacgaga aguaccacgu gcugaaguuc aaggggaacg cgauguggac guccgccguc      120

uucgagagcg cccagcaguc cgccgaccgc cggcucaacc gcaccgcccg gcgccggcug      180

gaccgcagcc agcagcggcg cgaccugcuc caggagcuga ucgcgcccgc caucaccccg      240

guggaccccc gguucuucga ccgcauccgg gaguccgccc ugugggccga ggacaagacc      300

acgggcagcc gcuucaccua cuucuccgac gagggguaca ccgacaagca guaccacaag      360

gaguacccca ccauccacca ccucaucugc agccugauga acgaccccgc gccgcacgac      420

ccccggcugg uguacaucgc cugcuccuac augcucacgc accgcggcca cuuccugaac      480

gaggccgaca aggagaagau cgacgacguc cuggacaucc ggcgcguguu caacgagcuc      540

ugcagcuggu ucaccuccua cggcaucgag gugcccuuca gcugcuuccc cgaggaguuc      600

gggaacgucc ugaccgccaa ccggggcgug caggaccgca cccgggccuu cuacgaccuc      660

cucuucgcgg ggaagaagcc gaagcccucc gaggacgacc agguggacau cgacguccug      720

aucaagcuga ucagcggcgg gaagaucgag cucuccaagc uguuccugaa cgaggaguac      780

agcgagcucg agaacaacaa gauccagcug uccgacgccg gcuucgacga gaaggagccc      840

gagcugcgca gcgagcucgg cgacgacuuc gggcuggugg ccgccgccaa gaacaucuac      900

gacugguccc ugcucacggg cgugcugggg gagugcaaga gcaucagcga ggcgaagguc      960

aagaccuacg agaagcacuc cgaggaccug gccgcccucc ggcgcguggu gcgcaaguac     1020

caccccgaca aguacaagga gaucuucaag aaguacgucg ugggcaacua caccagcuac     1080

uccggcaacg ucaagagcgc cgggaagauc uccgagacca agcugaagcg gugcaaccag     1140

gccgaguucu gcgacuaccu caagagcaag cugaaggacg ugaagccggc gcccggcgac     1200

gacgagugcg agcgcgugcu gaaggagauc uccaagcaca cguucugccc ccgccaggug     1260

accaccgaca accgggucau ccccuaccag cuguacuaca ccgagcucaa gaaggugcug     1320

gagaacgcca agcgguacau cccgcagcug aacgucacgg acgaguacgg gagcgccgcc     1380

gacaagaucc ucucccugau gaccuuccgc auccccuacu acgugggccc ccuggugaag     1440

aagaacgaca gcuccaacgc cuggaucguc cggcgcgggg agggccggau ccuccccugg     1500

aacuucgacg agcuggugga caaggacaag ugcgaggacg aguucauccg ccggaugacc     1560

ugcaagugca ccuacaucgc gggcaagaac gugcugccga agaacagccu ccuguacugc     1620

aaguucaugg uccugaacga gaucaacaac aucaagauca acggggagcg caucuccgug     1680

ggcgacaagc agaagaucua cacggagcug uucgagaaga agcggaaggu gaccguccgc     1740

gacauccgga accugcugac cagcgagggg cgccugaagg ccggcgacac cuucgggggc     1800

aucgacauca cggugaaguc cagccucaag ccccagcacg accugaagcg gcugauggcc     1860

gccggcguga ucaccgaggc ggacgccgag gagaucaucg agcgcaucac gugcaccacc     1920

gacgucuccc ggcuccgcac cuggcugggg aaguucggcc ugagcgccgc cgacgccaag     1980

uacguguccg ggcucaagua cagcgacuuc ggccgccuga gccgggaguu ccugaccgag     2040

gugaacgacg ucgaccccgc gaccggggag gugaucaacc ccaacaucau cacgaugaug     2100

ugggagaaca acaucaaccu cauggagcug cugucccaga aguacagcua ccgcucccag     2160

aucgagagca ugaaccggga guacuacgcc gccaacccgc ucacccugga cgagcgcaug     2220

uccgagaugu acauccccag cgccgugcgg cgccccguca agcggaccau cgagaugaug     2280

aaggaguaca ugcacaucau gcgcaagccc ccgaagcgga ucuucaucga gauggcccgc     2340

gagcugaccg accucgacaa caagggcaag cggaccucca gccgccggaa cacgcugcug     2400

gaguucuuca agaagugccc ccaggacacc accgagcucc gcgcgcagcu ggagaagaag     2460

uccgacgacg agcugcggag cgagaagcuc uaccuguacu ucgcccagcu gggcaagugc     2520

auguacuccg gggagagcau cgacaucgag cgccucggcu ccaagcugua cgacguggac     2580

cacaucuucc cccaggcccg ggugaaggac gacagcaucg agaacaaggu ccuggugcuc     2640

uccgaccuga acggggccaa gagcgaccgc uacccgauca acgccgacau ccggaacaag     2700

augcacuccu ucugggacaa gcugcacagc aucggccuca ucggcgagaa gaaguacgag     2760

cgccugaccc ggagcacggg guucaccgac gacgagcugg cgggcuucau cucccgccag     2820

cucguggaga cccgccagag caccaaggcc gucgccgccc ugcuggcgga ggaguucccc     2880

gaguccgaga ucguguacgu gaaggccggg cucgccagcc aguuccggca cgaguucggc     2940

cugcugaagu gccgcgagau caacgaccuc caccacgcca aggacgccua ccugaacauc     3000

gucaugggga acaucuacca cgugaaguuc acgaaggacc ccaugaacuu cgugaaggag     3060

uaccggcgca aggagcaggu cuucgaccug aagauccagu ccaugcucac ccacgacgcg     3120

auccggaacg gcgagaccgc cuggaucggc gacgggaccu ggcuggagac ggugaagaag     3180

cagauggaga agaacaacau ccgcuacgug cgguacagcu uccgccggaa gggcgcccug     3240

uucaaccuca ugcccgagcg caagaacccg ucccugcugc cccggaagaa cgggaagaac     3300

augguccucc ccgccgagaa guacggcggg uacaacaaca ccgccgcgag cuucuucucc     3360

cuggugaagu accacagcgc caagggcucc ggcgugguca ucauccccgu ggagcugauc     3420

cacgcccacc gcuacgagca cgacagcucc uucgccgagg aguacgcccu ccgggugcug     3480

gcggacaucg ucagccugaa gaagggggac gugcuccagu ccguggaguu cccgcugggc     3540

agccgcaucc ugaaggucaa cacccuccug gacaucgacg gguuccgggc caacaucacc     3600

ggcaaggacu ccgggggcaa guaccugggc aucagcuccg ccagcagccu caucaucucc     3660

cagguggaca cgaccuacau caagcgcgug aucagcauca acgagcggau cuccgccugc     3720

gagaagaaca agcagagcua caccauccgc cccgaguucg acgucaucac cuccgagcgg     3780

aacaucgaga ucuacaacau gcugugcgag aagaucagcu cccgccccuu cugcaucaag     3840

uucgcgaaca cgagcgacac cuucgugucc gggcgcgaga aguucaucgc ccugagccuc     3900

aacgagcagg ucaagacccu gaccaacaug cuguccgugu ucaagacggg ccgggccagc     3960

gggugcgacc ucaccaacau cggcgggucc aagaacaccg ccaucgugaa gcugaacgcc     4020

gugcugagca accucaagga cuucaagucc guccgcauca ucgaccagag ccccaccggc     4080

cuguacgaga aggugucccc gaaccugcuc agccuguga                            4119


<210>  1997
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1997
augaccaaga agaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucacgg acgacuacaa gauccccgcc aagaagauga aggugcucgg gaacaccgac      120

aaggaguaca ucaagaagaa ccugcugggc gcgcuccugu ucgacagcgg ggagaccgcc      180

gaggccaccc gccugaagcg gacggcccgc cggcgcuaca cccggcgcaa gaaccggcuc      240

cgcuaccugc aggagaucuu cgccgaggag aucgcgaagg ccgacaacuc cuucuuccag      300

cggcuggacg agagcuuccu caccgacgac gacaaggacu ucgacuccca cccgaucuuc      360

ggcaacaagg ccgaggagga cgccuaccac cagaaguuca gcaccaucua ccaccuccgc      420

aaguaccugg cggacucccc cgagaagucc gaccuccggc ugguguaccu ggcccucgcc      480

cacaugauca aguaccgcgg ccacuuccug aucgaggggg agcugaaggc cgagaacacg      540

gacguccaga agcuguucac cgacuucgug gagguguacg accggaccuu cgacgacagc      600

caccugagcg aggucaccgu ggacgccucc agcauccuga cggagaagau cuccaagagc      660

cgccggcucg agaaccugau cgaguccuac cccaaggaga agaagaacac ccuguucggc      720

aaccucaucg cgcugagccu ggggcuccag cccaacuuca agaccaacuu caagcugucc      780

gaggacgcca agcugcaguu cagcaaggac accuacgagg aggaccucga ggaggugcug      840

gagaagaucg gcgacgacua cgccgaccug uucacguccg ccaagaaccu cuacgacgcc      900

auccugcuga gcgggauccu caccgucgac gacagcucca ccaaggcgcc gcugagcgcc      960

uccaugauca agcgcuacgc cgagcaccac gaggaccugg agaagcucaa ggccuucauc     1020

aagaccaaca agcccaaccu guaccacgac aucuucaagg acgagacgca gaacggcuac     1080

gccggcuaca ucgagaacgg ggugaagcag gacgaguucu acaaguaccu gaagaccauc     1140

cucagcaaga ccgacggcuc cgaguacuuc cuggacaaga ucgagcggga ggacuuccug     1200

cgcaagcagc ggaccuucga caacgggagc aucccccacc agauccaccu ccaggagaug     1260

cacgcgaucc ugcgccggca gggcgacuac uaccccuucc ugaaggagaa ccaggaccgc     1320

aucgagaaga uccucacguu ccgcaucccg uacuacgugg gcccccuggc ccggaagaac     1380

ucccgcuucg ccugggccga guaccacagc gacgagaaga ucacccccug gaacuucaac     1440

aaggucaucg acaaggagaa guccgccgag aaguucauca cccggaugac ccugaacgac     1500

cuguaccucc ccgaggagaa ggugcugccg aagcacagcc acguguacga gacguucgcg     1560

gucuacaacg agcugaccaa gaucaaguac gugaacgagc aggggaagga ggccuucuuc     1620

gacuccaaca ugaagcagga gaucuucgac cacguguuca aggagaaccg caaggucacc     1680

aaggagaagc uccugaacua ccuggacaag gaguuccccg aguaccggau ccaggaccuc     1740

aucggccugg acaaggagaa caagagcuuc aacgccuccc uggggaccua ccacgaccuc     1800

aagaagaucc uggacaagag cuuccuggac gacaagguga acgaggaggu gaucgaggac     1860

aucaucaaga cgcucacccu guucgaggac cgcgagauga uccagcagcg gcugcacaag     1920

uacuccgaca ucuucagcac ccagcagcuc aagaagcugg agcgccggca cuacaccggc     1980

ugggggcgcc uguccuacaa gcucaucaac ggcauccgga acaaggagaa caacaagacg     2040

auccuggagu accugaucga cgacggcuac gccaaccgca acuucaugca gcucaucaac     2100

gacgacaccc ugcccuucaa gcagaucauc aaggacgcgc agaucaucgg gaacaucgac     2160

gacaucgccg ccgucgugca cgagcugccc ggcagcccgg ccaucaagaa ggggauccuc     2220

caguccauca agaucgugga cgagcugguc aaggugaugg gccacaaccc cgacaacauc     2280

gucaucgaga uggcccggga gaaccagacc accaaccgcg ggcggagcca gagccagcag     2340

cgccugaaga agcuccagga cucccugaag gagcugggca gcaacauccu caacaagaag     2400

aagcccuccu acguggagga caagguggag aacguccacc uccaggacga ccggcuguuc     2460

cuguacuaca uccagaacgg caaggacauc uacaccgggg aggagcugga caucgaccgc     2520

cugagcgacu acgacaucga ccacaucauc ccccaggcgu ucaucaagga cgacuccauc     2580

gacaaccggg ugcucacgag cuccgccaag aaccgcggca agagcgacga cgugccgaac     2640

cuggacaucg uccgcgcccg gaaggccgac ugggugcgcc uguacaaguc cgggcucauc     2700

agcaagcgga aguucgacaa ccugaccaag uccgagcgcg gcgggcugac cgaggccgac     2760

aaggcgggcu ucaucaagcg gcagcucgug gagacccgcc agaucacgaa gcacgucgcc     2820

cagauccugg acgcccgguu caacaccgag cacgacgagg acgacaaggu gauccgcaac     2880

gugaagguca ucacccucaa gagcaaccug aucucccagu uccggaagga cuucaaguuc     2940

uacaaggugc gcgagaucaa cgacuaccac cacgcccacg acgccuaccu gaacgcggug     3000

gucggcaccg cccugcuccg gaaguacccc aagcuggccc ccgaguucgu guacggggag     3060

uacaagaagu acgacgugcg caagcugauc acgagcucca gcgacaaggc caccgcgaag     3120

uacuucuucu acuccaaccu caugaacuuc uucaagcggg ucgugcgcua cagcaacggc     3180

cgggugaucg uccgccccgu gaucgaguac agcaaggaca ccggggagau cguguggaac     3240

aaggagaccg acuuccggac gaucugcaag guccuguccu acccccaggu gaacaucgug     3300

aagaaggucg agagccagac cggcggcuuc uccaaggaga gcauccugcc gaagggggac     3360

uccgacaagc ucaucccccg caagaccaag aagguguacu gggacaccaa gaaguacggc     3420

ggguucgaca gccccaucau cgccuacucc guguucgucg uggccgacgu ggagaagggc     3480

aaggccaaga agcugaagaa ggucaaggag cuggugggga ucagcaucau ggagcggccg     3540

uccuucgaga agaaccccau cguguuccuc gagaagaagg gcuaccgcaa cguccaggag     3600

gacacgcuga ucaagcugcc caaguacagc cucuucgagu ucgagggcgg gcggcgccgc     3660

cugcuggccu ccgcgagcga gcuccagaag ggcaacgagg uggugcuguc cgaccggcug     3720

gucgagcucc uguaccacgc ccaccgcgug aacagcuuca acuccaccga gcaccugaag     3780

uacgugagcg agcacaagaa ggaguucggg gagguccucu ccugcgugga gaacuucgcc     3840

aacagcuacg uggacgucga gaagaaccug gacaagaucc gggccgugau caacaagauc     3900

gacaccuucu ccaucgagga gaucagcacc agcuucguga accugcucac gcugaccgcc     3960

cugggcgcgc cggccgacuu caacuuccuc gggaagaaga ucccccgcaa gcgguacacc     4020

uccaccaagg agugccugaa cgccacgcug auccaccaga gcaucaccgg ccucuacgag     4080

acccgcaucg accuguccaa gcugggcgag gacaaguga                            4119


<210>  1998
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1998
augaccaaga agaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucacgg acgacuacaa ggugcccgcc aagaagauga aggugcucgg gaacaccgac      120

aagaaguaca ucaagaagaa ccugcugggc gcgcuccugu ucgacagcgg ggagaccgcc      180

gaggccaccc gccugaagcg gacggcccgc cggcgcuaca cccggcgcaa gaaccggcuc      240

cgcuaccugc aggagaucuu cgccaacgag aucgcgaagg ucgacgaguc cuucuuccag      300

cggcuggacg agagcuuccu caccgacgac gacaagaccu ucgacuccca cccgaucuuc      360

ggcaacaagg ccgaggagga cgccuaccac cagaaguucc ccacgaucua ccaccuccgc      420

aagcaccugg ccgacagcuc cgagaaggcg gaccugcggc ugguguaccu ggcccucgcc      480

cacaugauca aguuccgcgg ccacuuccug aucgaggggg agcugaacgc cgagaacacc      540

gacgugcaga agaucuucgc cgacuucguc ggcguguaca accggaccuu cgacgacagc      600

caccucuccg agaucaccgu ggacgucgcg agcauccuga cggagaagau cuccaagagc      660

cgccggcugg agaaccucau caaguacuac cccaccgaga agaagaacac ccuguucggg      720

aaccugaucg cccucgcccu gggccugcag cccaacuuca agaccaacuu caagcucucc      780

gaggacgcca agcugcaguu cagcaaggac acguacgagg aggaccugga ggagcuccug      840

gggaagaucg gcgacgacua cgccgaccug uucaccuccg cgaagaaccu cuacgacgcc      900

auccugcuga gcggcauccu caccguggac gacaacucca ccaaggcccc gcugagcgcc      960

uccaugauca agcgcuacgu ggagcaccac gaggaccugg agaagcucaa ggaguucauc     1020

aaggccaaca agagcgagcu guaccacgac aucuucaagg acaagaacaa gaacggguac     1080

gcgggcuaca ucgagaacgg ggucaagcag gacgaguucu acaaguaccu gaagaacauc     1140

cucagcaaga ucaagaucga cggcuccgac uacuuccugg acaagaucga gcgggaggac     1200

uuccugcgca agcagcggac guucgacaac ggcagcaucc cccaccagau ccaccuccag     1260

gagaugcacg ccauccugcg ccggcagggg gacuacuacc ccuuccugaa ggagaagcag     1320

gaccgcaucg agaagauccu caccuuccgc auccccuacu acgugggccc gcuggugcgg     1380

aaggacuccc gcuucgccug ggccgaguac cggagcgacg agaagaucac ccccuggaac     1440

uucgacaagg ucaucgacaa ggagaagucc gccgagaagu ucaucacccg caugacgcug     1500

aacgaccugu accuccccga ggagaaggug cugcccaagc acagccacgu guacgagacc     1560

uacgcggucu acaacgagcu gaccaagauc aaguacguga acgagcaggg gaaggagucc     1620

uucuucgaca gcaacaugaa gcaggagauc uucgaccacg uguucaagga gaaccggaag     1680

gucaccaagg agaagcuccu gaacuaccug aacaaggagu ucccggagua ccgcaucaag     1740

gaccucaucg gccuggacaa ggagaacaag uccuucaacg ccagccuggg gacguaccac     1800

gaccucaaga agauccugga caaggccuuc cuggacgaca aggugaacga ggaggugauc     1860

gaggacauca ucaagacccu cacccuguuc gaggacaagg acaugaucca cgagcggcug     1920

cagaaguacu ccgacaucuu caccgccaac cagcucaaga agcuggagcg ccggcacuac     1980

acgggcuggg gccgccugag cuacaagcuc aucaacggga uccggaacaa ggagaacaac     2040

aagaccaucc uggacuaccu gaucgacgac ggcuccgcga accgcaacuu caugcagcuc     2100

aucaacgacg acacccugcc cuucaagcag aucauccaga agucccaggu cgugggggac     2160

gucgacgaca ucgaggccgu ggugcacgac cugcccggca gccccgccau caagaagggg     2220

auccuccaga gcgucaagau cguggacgag cuggugaagg ucaugggcgg caacccggac     2280

aacaucguga ucgagauggc ccgggagaac cagaccacga accgcgggcg gucccagagc     2340

cagcagcgcc ugaagaagcu ccagaacucc cugaaggagc ugggcagcaa cauccucaac     2400

gaggagaagc ccagcuacau cgaggacaag guggagaacu cccaccucca gaacgaccag     2460

cuguuccugu acuacaucca gaacgggaag gacauguaca ccggcgacga gcuggacauc     2520

gaccaccuga gcgacuacga caucgaccac aucauccccc aggccuucau caaggacgac     2580

uccaucgaca accggguccu caccagcucc gcgaagaacc gcggcaagag cgacgacgug     2640

cccucccugg acaucgugcg ggcccgcaag gccgaguggg uccgccugua caagagcggg     2700

cucaucucca agcggaaguu cgacaaccug accaaggccg agcgcggcgg gcugaccgag     2760

gccgacaagg cgggcuucau caagcggcag cucguggaga cgcgccagau caccaagcac     2820

guggcccaga uccuggacgc ccgguucaac accgagcacg acgagaacga caaggucauc     2880

cgcgacguga aggugaucac ccucaagagc aaccuggucu cccaguuccg gaaggacuuc     2940

gaguucuaca aggugcgcga gaucaacgac uaccaccacg cccacgacgc cuaccugaac     3000

gcgguggucg ggacggcccu gcucaagaag uacccgaagc uggccagcga guucguguac     3060

ggcgaguaca agaaguacga cauccggaag uucaucacca acuccagcga caaggccacc     3120

gcgaaguacu ucuucuacuc caaccugaug aacuucuuca agaccaaggu gaaguacgcc     3180

gacggcacgg ucuucgagcg ccccaucauc gagaccaacg ccgacgggga gaucgccugg     3240

aacaagcaga ucgacuucga gaaggugcgg aaggugcuca gcuaccccca ggucaacauc     3300

gugaagaagg uggagaccca gaccggcggg uucuccaagg agagcauccu gcccaagggc     3360

gacagcgaca agcugauccc gcgcaagacg aagaaggucu acugggacac caagaaguac     3420

gggggcuucg acucccccac cguggccuac agcguguucg ucguggcgga cguggagaag     3480

ggcaaggcca agaagcucaa gaccgucaag gagcuggugg ggaucuccau cauggagcgg     3540

agcuucuucg aggagaaccc cguggaguuc cuggagaaca agggcuacca caacauccgc     3600

gaggacaagc ucaucaagcu gcccaaguac ucccuguucg aguucgaggg cgggcggcgc     3660

cggcuccugg ccagcgccuc cgagcugcag aaggggaacg agaugguccu cccgggcuac     3720

cugguggagc ugcucuacca cgcccaccgc gcggacaacu ucaacagcac ggaguaccug     3780

aacuacgugu ccgagcacaa gaaggaguuc gagaaggucc ugagcugcgu ggaggacuuc     3840

gccaaccucu acguggacgu cgagaagaac cuguccaaga uccgggccgu ggccgacagc     3900

auggacaacu ucuccaucga ggagaucagc aacuccuuca ucaaccugcu cacccugacc     3960

gcccugggcg cgcccgccga cuucaacuuc cucggggaga agaucccccg caagcgguac     4020

accagcacga aggagugccu gaacgccacc cugauccacc aguccaucac cggccucuac     4080

gagacccgca ucgaccugag caagcugggg gaggaguga                            4119


<210>  1999
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  1999
augaacagcg acaagcgcga ggacuacuac gugggccugg acauggggac cggcucccuc       60

gggggcgccg ucaccgacac gcaguaccac cuccugaagg ugaaggggaa ggacuucugg      120

uucgugcggg aguacgagac cgccaagagc cagcuggacc gccggaccca ccgcaucgcg      180

aagcggcgcc ugcagcggca caaggcccgc aucggccuga uccgguccua cuucgccgag      240

gacauccuca agcacgaccc ccuguucuac auccgccagg acaacagcaa guacuaccac      300

gaggacaagg acguccggcu gaccacgaag gacuccaucu ucgccgaccc gggcuacggg      360

gacaaggagu accucgacga caaggaguac cccaccaucu uccaccuccg ccaggcccug      420

cugcgggacc agaucaagga cgaggagcgc uacagccggu uccuguaccu ggcgaucauc      480

aacauguucg agcaccgcgg ccacuuccuc cugaacaccg aguccagcga ccugaacgcc      540

gacaugauca aggacguggg ggagcucgug augaacgcca ucugcggcgu cuccgaguac      600

gggagcguga ccuacaagga gaucaucgag auccuggagg acaagaacau cucccggacg      660

gccaagaagg accagaucgc gagcaagauc gacaucaaga aguccgagaa ggcccagaug      720

gagcgcauca agugccugug cggcauggag gccgacgccc gggugauguu cgagaucgag      780

gccgacgaga agaucaagau cgcguuccag caggccgccu ucgaggacga gaaggaggag      840

aucgaggccg ucaucggcga ggaccgcuac cucaucaucg agaacaugaa gcagcuguac      900

gacuacagcc agcugcaguc cgugcucaac ggguucgacu accugagcga cgcccgggug      960

cagauguacg agaagcacaa ggaggaccug caccuccuga agaaggucua ccgcaagaac     1020

cuggaccgcg agaucuacga ccggauguuc cgcuccggcg aggaggggag cuacugcgcg     1080

uacuccaaca gcaccaacag cguggacucc cucgccgagg ccaagaagua ccggcgcaac     1140

augaagaagc ggagccagaa ggaccuguac gaccgcauca agaaggaccu gaaggaggug     1200

cacgacgccg acgccgacgc gauccggaag gagauggagc ucgagcgcuu ccugcccaag     1260

cagaucaccg gcgccaacgg caucaucccc aaccaggucc accggaagga gcugaagaag     1320

auccucgaga acgccgagca ccaccuggac uuccugaagg agaaggacga guccggguac     1380

accgugagcg agcgcauccu caugcuguuc uccagcaaca ucccguacua cgugggcccc     1440

gucggguccg gcagcaagac ggggugggcc aagauccagg aguccggcca ggugcugccc     1500

uggaacaugg agcagaagau cgacaucgag cagaccagcg agcgguucau caccaaccuc     1560

auccgcgagu gcaccuaccu guccggcgag aaggugcugc ccaagcagag ccuccuguac     1620

gaguccuacu gcguccugaa cgagaucaac aaccuccgga uccacgggaa gcgcaucgug     1680

ccggagcuga agcaggacau cuacaaggag guguuccagg gcuuccggcu ggggaagaag     1740

gucacgaaga agaacaucug caccuaccug aucaaccgcg gccucaucac cgaggagagc     1800

gagguguccg ggaucgacaa ggagaucaac gccuaccugc ccagcuacgg caagauguac     1860

ggcgucuucg gggagcggcu gcgcgaggag gcggugcagg agaucgccga gaagaucauc     1920

uacuacggca ccaucuacgg ggacgccaag uccauguucc ggaagaagcu caaccaguac     1980

gugagcgacg gcuggaugac ggaguaccag gccaagcgca ucuccgggua caaguucaag     2040

gacugggcgc gccugagccg gagcaugcug ugccucuccg gcugcgacaa gagcaccggc     2100

gagaagaucu cccugauccg cgccaugugg gaguacaacc ugaacuucau ggagcucauc     2160

uaccgggagg acuucaccuu ccagaaggag cuggaggagc gccggaaggc ccuggggaag     2220

acccuccagg aguuccaguu cgaggaccug gacgaguacu acuacagcgc ccccgucaag     2280

cgcaugaucu ggcaggccgu gcaggcgcug aaggagauca cgcagaucau gggccacaac     2340

cccaagcgga ucuucaucga gaugacccgc accgaggagg agaaggggga gcagggccgg     2400

aagaacuccc gcgagaagcg gcuccugaag auguacgaga gcauccgcga cgagcggaac     2460

ugggccgccg agaucaagaa cgccaacgcc uccggcaagc ugaacagcaa gaagcucuac     2520

cuguacuacc ugcagauggg gcgcgacgug uacaccggcg agccgaucga caucgaggac     2580

cucuucgacg acucccggua cgacaucgac cacaucuacc cccgcagccu gacgaacgac     2640

aacaacaucg agaacaaccu gguccucgug uccaagacca ucaaccagaa cgagaagaag     2700

aacgacuacc ccgugcccga gaagauccgg cgcaacaaga agguccggaa ccuguggcac     2760

acccugcaca agaugggguu caugaacgac gagaaguaca accgccucac cagcacggag     2820

cggcuguccg aggaccagcu ggcgggcuuc aucgcccgcc agcucgugga gaccgcccag     2880

ggggccaagg gcaucgccga gcugaugaag gcgaugcugc cgggcaccga caucguguac     2940

gccaaggccc gcaacgucag cgacuuccgg aaggaguaca agaugcucaa gucccgccug     3000

gugaacgacu accaccacgc ccaggacgcg uaccugaaca ucguggucgg gaacguguac     3060

uucaugaagu ucacccggaa ccccaugaac uucgugaaga cggaggcccg ccgggacggc     3120

cgcaacuacg acuacaaccu cuacaagaug uacagcaagg acaucauccg gaacggggag     3180

aaggccugga ucgccaccuc cgagcagggc cccgggacca uccgccuggu caaggagacc     3240

augggccgga acacccccau caucacgcgc caggccuucg agcagcgggg cgagcuguuc     3300

aaccuccagc cgguggggaa guacagcgcg aagaaggaca acuacgugcc ccugaagauc     3360

aacgacgaga agaugcagga cgucagcaag uacggcgggu acaccucccu gaaccccagc     3420

uacuucaucu ucaucgagca cggccccgag aagaagcgca agaagugcuu cgaggugauc     3480

cacuccuacu acgccgccca gaucaagacc gagaaggacc ucaucgacuu ccugcugcag     3540

aagggguaca agaacccgcg ggugaucaac gcccgcauca agaagaacgc ccucaucaag     3600

uacaacggcu acuuccugua caucaucggc auggacgcgc ggaagaacau cgaguucagc     3660

aacgccaccg ccaugugccu gaagaacaag uacauccagu acgucugcaa gcucgagaag     3720

augaacaagg ccauccugcu guccgagaag cagaagacga accuccacug ggacgagaag     3780

aucacgugcg agagcaaccu ggagcuguac cgcgagcuca ccgagaagca ccuccacucc     3840

aucuaccagc ggcacccccg cagcaucggg aagugccugg ccgacggcga gggggcguuc     3900

aagcugcugg acaucgagga gcaggucaag aucaucugcg acaucgugca guacaccucc     3960

uuccagcggg gcguguucag ccugaaggug cucgggggcc ccaaggaggu gggccgcauc     4020

cgcaucuccg ggaacaugac cgaggccaag gagugcaagc uggucaacua cagcaucacc     4080

ggcauguaca agaccgagau ggaccugcuc aagaaguga                            4119


<210>  2000
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2000
auggagaaga ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcguc       60

aucacggacg acuacaaggu gcccgccaag aagaugaagg ugcucgggaa caccgacaag      120

aaguacauca agaagaaccu gcugggcgcc cuccuguucg acuccgggga gaccgcggag      180

gucacccgcc ugaagcggac ggcccgccgg cgcuacaccc ggcgcaagaa ccggcuccgc      240

uaccugcagg agaucuucgc caaggagaug gccaaggugg acgagagcuu cuuccagcgg      300

cuggaggagu ccuuccucac cgacgacgac aagaccuucg acagccaccc gaucuucggc      360

aacaaggccg aggaggacgc guaccaccag gaguucccca cgaucuacca ccuccgcaag      420

caccuggccg acuccagcga gaaggccgac cuccggcugg uguaccuggc ccucgcgcac      480

augaucaagu uccgcggcca cuuccugauc gagggggagc ugaacgccga gaacaccgac      540

auccagaagc uguucaacga cuucgucgag accuacgaca agaucgccga cgagucccac      600

cugagcgagg uggacgccuc cagcauccug accaacaaga ucuccaagag ccggcgccuc      660

gagaaccuga ucaagcagua ccccacggag aagaagaaca cccuguucgg caaccucgug      720

gcccuggucc uggggcucca gcccaacuuc aagaccaacu ucaagcuguc cgaggacgcg      780

aagcugcagu ucagcaagga caccuacgac gaggaccucg aggagcugcu gggcaagauc      840

ggggacgacu acgccgaccu cuucacggcc gccaagaacc uguacgacgc cauccugcuc      900

uccggcaucc ugaccgugga cgacaacagc accaaggcgc cgcuguccgc cagcaugauc      960

aagcgguacg aggagcacca cgaggaccuc gagaagcuga agaaguucau caaggugaac     1020

aacuucgaca aguaccacga gaucuucaag gacaagagca agaacggcua cgccggguac     1080

aucgagaacg gcgucaagca ggacaucuuc uacaagcacc ugaaguccau caucagcgag     1140

aagaacgggg gccaguacuu ccucgacaag aucgagcgcg aggacuuccu gcggaagcag     1200

cgcaccuucg acaacggcuc caucccccac cagauccacc uccaggagau gcgggccauc     1260

cuccgccggc agggggagua cuaccccuuc cugaaggaga accaggccaa gaucgagaag     1320

auccugacgu uccgcauccc cuacuacgug ggcccgcucg cgcgcaagga cagccgguuc     1380

gccugggcca acuaccacuc cgacgagccc aucacccccu ggaacuucga cgaggugguc     1440

gacaaggaga aguccgccga gaaguucauc acccgcauga cccugaacga ccuguaccug     1500

cccgaggaga aggugcuccc gaagcacagc cacguguacg agacguucac cgucuacaac     1560

gagcugacca agaucaagua cgugaacgag cagggggaga gcuucuucuu cgacgccaac     1620

augaagcagg agaucuucga ccacguguuc aaggagaacc ggaaggucac caaggcgaag     1680

cugcucuccu accugaacaa cgaguucgag gaguuccgca ucaacgaccu gaucggccuc     1740

gacaaggaca gcaaguccuu caacgccagc cuggggacgu accacgaccu gaagaagauc     1800

cucgacaagu ccuuccugga cgacaagacc aacgagcaga ucaucgagga caucgugcug     1860

acccucaccc uguucgagga ccgggacaug auccacgagc gccugcagaa guacagcgac     1920

uucuucacgu cccagcagcu caagaagcug gagcggcgcc acuacaccgg cuggggccgg     1980

cugagcuaca agcucaucaa cgggauccgc aacaaggaga acaacaagac cauccuggac     2040

uuccugaucg acgacggcca cgccaaccgg aacuucaugc agcucaucaa cgacgagucc     2100

cugagcuuca agaccaucau ccaggaggcc cagguggucg gggacgugga cgacaucgag     2160

gcggucgugc acgaccugcc cggcuccccc gccaucaaga aggggauccu ccagagcgug     2220

aagaucgucg acgagcuggu gaaggugaug ggcgacaacc ccgacaacau cgucaucgag     2280

auggcccgcg agaaccagac gaccaaccgg gagcgcagcc agucccagca gcggcugaag     2340

aagcuccaga acagccugaa ggagcugggc uccaacaucc ucaacgagga gaagccgagc     2400

uacaucgagg acaaggugga gaacucccac cuccagaacg accagcuguu ccucuacuac     2460

auccagaacg ggaaggacau guacaccggc gacgagcugg acaucgacca ccugagcgac     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acaacuccau cgacaaccgc     2580

gugcucacca gcuccgccaa gaaccggggc aagagcgacg acguccccuc ccuggacauc     2640

gugcgcgcgc ggaaggccga gugggugcgc cuguacaaga gcgggcucau cuccaagcgc     2700

aaguucgaca accugaccaa ggccgagcgc gggggccuga cggaggccga caaggccggc     2760

uucaucaagc ggcagcucgu cgagacccgg cagaucacca agcacguggc gcagauccug     2820

gacgcccgcu ucaacaccaa gcgggacgag aacgacaagg ugauccgcga cgucaaggug     2880

aucacgcuca agagcaaccu ggugucccag uuccggaagg aguucaaguu cuacaagguc     2940

cgcgagauca acgacuacca ccacgcccac gacgccuacc ugaacgccgu gguggggacc     3000

gcgcugcuca agaaguaccc caagcugacc ccggaguucg ucuacggcga guacaagaag     3060

uacgacgugc ggaagcugau cgccaagagc uccgacgacu acagcgagau ggggaaggcc     3120

accgccaagu acuucuucua cagcaaccuc augaacuucu ucaagacgga ggugaaguac     3180

gcggacggcc gcgucuucga gcggcccgac aucgagacca acgccgacgg cgagguggug     3240

uggaacaagc agaaggacuu cgacaucguc cgcaaggugc uguccuaccc ccaggugaac     3300

aucgucaaga agguggagga gcagaccgug gggcagaacg gcgggcuguu cgacaacaac     3360

auccucgcca agcccgagaa ggacgacaag aagaagcugu ucccgaucaa gaagucccug     3420

agcaccacga gcuacggcgg guacgcccgg gccaccaucg cguacuccau ccucaucguc     3480

agcgaguucg gcaagaacaa ggagcugaag cugcucggcc ugccccugcu cgacaaggug     3540

aaguacgaga agaaccccau cgccuaccug aacuccaucg ggaucaucaa cgucaagagc     3600

aucuucaagc ugcccaagua cugccucuuc gaguuccagg agaagaacac caacuaccgc     3660

cgguacaugg uguccaacca ggagcugaag cgcgccaacc agcuguuccu gaccugcaag     3720

gagacggagc ugguguacaa guacaucaag agcgacaacg acaacaccca ccuggugggc     3780

aacgagaaga ccauccucuc caucuggaac aagcugguca ucuucaugga gcggaccaag     3840

cugaucgaca agaagcuguu cgccagccug aagcagcuga aggacgaggu ggaccucauc     3900

aacauccugg acaugacggc caagucccug gacuucacca agaucagcgc ggggcagaag     3960

aagaucacca ucuccgacag ccucucccug ccgaucaagc gcuggcaguu cagcuccgac     4020

gagaagaugc ugaagcagcu caagaccgcc acgcugaucc accagagcgu gaccggccug     4080

uacgagaccc ggaucgaccu cagcaagcug ggggaggagu ga                        4122


<210>  2001
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2001
auggagaaga ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcguc       60

aucacggacg acuacaaggu gcccgccaag aagaugaagg ugcucgggaa caccgacaag      120

aaguacauca agaagaaccu gcugggcgcc cuccuguucg acuccgggga gaccgcggag      180

gucacccgcc ugaagcggac ggcccgccgg cgcuacaccc ggcgcaagaa ccggcuccgc      240

uaccugcagg agaucuucgc caaggagaug gccaaggugg acgagagcuu cuuccagcgg      300

cuggaggagu ccuuccucac cgacgacgac aagaccuucg acagccaccc gaucuucggc      360

aacaaggccg aggaggacgc guaccaccag gaguucccca cgaucuacca ccuccgcaag      420

caccuggccg acuccagcga gaaggccgac cuccggcugg uguaccuggc ccucgcgcac      480

augaucaagu uccgcggcca cuuccugauc gagggggagc ugaacgccga gaacaccgac      540

auccagaagc uguucaacga cuucgucgag accuacgaca agaucgccga cgagucccac      600

cugagcgagg uggacgccuc cagcauccug accaacaaga ucuccaagag ccggcgccuc      660

gagaaccuga ucaagcagua ccccacggag aagaagaaca cccuguucgg caaccucgug      720

gcccuggucc uggggcucca gcccaacuuc aagaccaacu ucaagcuguc cgaggacgcg      780

aagcugcagu ucagcaagga caccuacgac gaggaccucg aggagcugcu gggcaagauc      840

ggggacgacu acgccgaccu cuucacggcc gccaagaacc uguacgacgc cauccugcuc      900

uccggcaucc ugaccgugga cgacaacagc accaaggcgc cgcuguccgc cagcaugauc      960

aagcgguacg aggagcacca cgaggaccuc gagaagcuga agaaguucau caaggugaac     1020

aacuucgaca aguaccacga gaucuucaag gacaagagca agaacggcua cgccggguac     1080

aucgagaacg gcgucaagca ggacaucuuc uacaagcacc ugaaguccau caucagcgag     1140

aagaacgggg gccaguacuu ccucgacaag aucgagcgcg aggacuuccu gcggaagcag     1200

cgcaccuucg acaacggcuc caucccccac cagauccacc uccaggagau gcgggccauc     1260

cuccgccggc agggggagua cuaccccuuc cugaaggaga accagagcaa gaucgagaag     1320

auccugacgu uccgcauccc cuacuacgug ggcccgcucg cccgcaagaa cucccgguuc     1380

gcgugggcca aguaccacag cgacgagccc aucacccccu ggaacuucga cgaggugguc     1440

gacaaggaga aguccgccga gaaguucauc acccgcauga cccugaacga ccuguaccug     1500

cccgaggaga aggugcuccc gaagcacagc cacguguacg agacguacgc cgucuacaac     1560

gagcugacca agaucaagua cgugaacgag caggggaagg aguccuucuu cgacagcaac     1620

augaagcagg agaucuucga ccacguguuc aaggagaacc ggaaggucac caaggagaag     1680

cugcucaacu accugaacaa ggaguucccc gaguaccgca ucaaggaccu gaucggccuc     1740

gacaaggaga acaaguccuu caacgccagc cuggggaccu accacgaccu gaagaagauc     1800

cucgacaagu ccuuccugga cgacaaggug aacgaggagg ugaucgagga caucaucaag     1860

acgcugaccc ucuucgagga caaggacaug auccacgagc ggcugcagaa guacagcgac     1920

aucuucaccg cgaaccagcu gaagaagcuc gagcgccggc acuacaccgg cuggggccgc     1980

cuguccuaca agcugaucaa cgggauccgg aacaaggaga acaacaagac gauccucgac     2040

uaccugaucg acgacggcag cgccaaccgc aacuucaugc agcugaucaa cgacgacacc     2100

cuccccuuca agcagaucau ccagaagucc caggucgugg gggacgucga cgacaucgag     2160

gccguggugc acgaccugcc cggcagcccg gccaucaaga aggggauccu gcaguccguc     2220

aagaucgugg acgagcucgu gaaggucaug ggcggcaacc ccgacaacau cgugaucgag     2280

auggcgcggg agaaccagac caccaaccgc gggcggagcc agagccagca gcgccugaag     2340

aagcugcagu ccagccucaa ggagcugggc uccaacauca ucaacgagga gaagcccagc     2400

uacaucgagg acaaggugga gaacucccac cuccagaacg accagcuguu ccuguacuac     2460

auccagaacg ggaaggacau guacacgggc gacgagcugg acaucgacca ccucagcgac     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacuccau cgacaaccgg     2580

guccugacca gcuccgccaa gaaccgcggc aagagcgacg acgugccguc ccuggacauc     2640

gugcgggccc gcaaggccga guggguccgg cucuacaaga gcgggcugau cuccaagcgc     2700

aaguucgaca accugaccaa ggcggagcgc ggcgggcuca ccgaggccga caaggccggc     2760

uucaucaagc ggcagcuggu ggagacccgc cagaucacga agcacguggc ccagauccug     2820

gacgcccggu ucaacaccga gcacgacgag aacgacaagg ucauccgcga cgugaaggug     2880

aucacccuca agagcaaccu ggucucccag uuccggaagg acuucgaguu cuacaaggug     2940

cgcgagauca acgacuacca ccacgcgcac gacgccuacc ugaacgccgu ggucgggacc     3000

gcccuccuga agaaguaccc caagcuggcc cccgaguucg uguacggcga guacaagaag     3060

uacgacgugc ggaagcucau cgccaagagc uccgacgacc acagcgagau gggcaaggcg     3120

acggccaagu acuucuucua cagcaaccug augaacuucu ucaagaccaa ggucaaguac     3180

gccgacgggu ccguguacga gcgccccauc aucgagacca acgcggacgg cgaggugguc     3240

uggaacaagc agaaggacuu cgagaucauc cggaaggugc ugagcuaccc ccaggugaac     3300

aucgucaaga agguggagga gcagaccgug gggcagaacg gcggccucuu cgacaacaac     3360

auccuggcca agccggagaa ggacgacaag aagaagcugu uccccaucaa gaagucccuc     3420

agcacgaccu ccuacggggg cuacgcccgc gccaccaucg ccuacagcau ccugaucguc     3480

uccgaguucg ggaagaacaa ggagcugaag cuccugggcc ugccccuccu ggacaaggug     3540

aaguacgaga agaacccgau cgcguaccug aacagcaucg ggaucaucaa cgugaagucc     3600

aucuucaagc uccccaagua cugccuguuc gaguuccagg agaagaacac caacuaccgg     3660

cgcuacaugg ucagcaacca ggagcugaag cgggccaacc agcuguuccu gacgugcaag     3720

gagaccgagc ugguguacaa guacaucaag uccgacaacg acaacaccca ccucgugggc     3780

aacgagaaga ccauccugag caucuggaac aagcugguca ucuucaugga gcgcacgaag     3840

cucaucgaca agaagcuguu cgccucccug aagcagcuca aggacgaggu gaaccugauc     3900

aacauccugg acaucgccag caaguccauc gacuucacca agaucagcgc cgggcagaag     3960

aagaucgugc ucaacgacuc ccugagccug cccaucaagc gguggcaguu caaguccgac     4020

gagaagaugc ucaagcagcu gaacgacgcg acccugaucc accagagcau caccggccuc     4080

uacgagacgc gcaucgaccu gagcaagcug ggcgaggagu ga                        4122


<210>  2002
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2002
augaaccugc gccgggcccg cgacuacagc aucggccucg accuggggac cggcuccgug       60

gggugggccg ucaccgacgc ggagggcaac cuguacagcu ucaaggggcg gcccgccaug      120

ggcucccgca cguucccgaa cgccgagccc gccagcgugg cccggauccc ccgcggccag      180

cggcgccggu acgagcgccg gcgccagcgg aucaagcucc ugcagcaguu cuucaugucc      240

gacauggagc gcguggaccc cgacuucuuc acccggcuga accagagcac ccuccuggcg      300

gaggacaagg acgagcgcau cgccuccugc gccuggccga ucuucaacag cuccgacuuc      360

accgagaagg acuacuacaa gaaguucccc acgaucuacc accuccgggc cuuccugugc      420

gagagcgacg agaaggggga ccugcgccug gucuaccucg cgcugcacaa caucgugaag      480

caccggggca acuuccugua ccaggacaac cccgcccucu ccgccagcga cgccuccgug      540

gacgaggccg uccgccagcu gguggacgcg cuggccgagu ggugccagaa caaggagauc      600

gagcucagcu gcgacgccgg gcggaucauc gagguguucg gcgacgccuc ccugcgccgc      660

accgacaagc gggaccagcu gaaggggcuc cugggccugc ccgccgaccu cgcgaagacc      720

augggcaagg agcuggccag cgccguccug ggguacgccg ccaacuucuc caagaucuuc      780

cucaucagcg acgcggagca caaguucucc cugggcaacg acgagaaggu ggaggaguuc      840

gagaccaucu gcccgaacga gugcacgagc cuguucagcg cccuccgcac cgccuacucc      900

gccuaccugc ugagcgggau ccuccgggac gccaacggca agacccuguc cuucugcaag      960

aagaaggagu acgaccgcua ccacgacgac cuggcgcucc ugaaggcccu ggugcgggag     1020

uacgcccccg agagcuacca guccuucuuc ggcggggagc gccucgucgu gaacggccgg     1080

augaccaagc gcuacgaccc cacgaaggcc aagggguaca ccaaguacga ccuggugcac     1140

agcgcgggcu ccuacgacga gcuggccaag gacgucaaga agcuccuggg gagccuggcc     1200

gccgacgacu cccgguacca gcgcaugaug caggccuucg acgaggagcg guuccuccgc     1260

cggcugaaga ccagcgacaa cggcuccauc cccuaccagc ugcaccucga ggagcugcgc     1320

gcgaucaucg cccggcaggg cgaguucuac ccguuccuga aggaggagag caagaagauc     1380

gagucccugg ugagcuuccg cauccccuac uacguggggc cccucaccac gaagaacgcc     1440

gccgucgacg ugcacggcaa caagcgguuc gccugguccg agcgcaagcc cgggauggag     1500

agcgcgaagg ugcggccgug gaacugggac gacgucaucg accgcggcaa guccgccgag     1560

aacuucaucg ugcgcaugac cgggaccugc accuaccugc agaacgagaa ggugcugccc     1620

aagugcagcc uccuguacga ggaguucugc guccugaacg agcucaacgg cgcccacugg     1680

uccgccgacg gcgaccacga gugccccuuc gacacgagcg accggcgcgg gaucgugcag     1740

gaccuguucu gcggcaagcg gcgcgucagc uacaagcagg ucgaggacug gcugcggcag     1800

cacggguccc gccgggcccg cgugagcggc gggcagggcg cguccggcuu cgagagcaag     1860

auguccagcu acaucuucuu cuccaaggac gugcuccacg uggaccggau cgaccccgcc     1920

gacuucccga ugaucgagga gaucauccug uggaacaccc uguucgagga ccgcgacauc     1980

cucaaggagc ggaucgagga ggccuacggg gaccgccuga acgccgagca gaucaagacc     2040

aucugcaaga agcgguucac cggcuggggg cgccugagcc ggcgccuccu gacgggcauc     2100

aaggcgcaga ccgaccaggg cgaccugucc aucauggaca uccuccggga gggggacccc     2160

aacaacgacc accgcggcaa ggccauggug uucaugggga uccugcacag cgaggaccug     2220

ggcuuccaga agaagaucga ccagaucaac caggagaagg ucuccgccgg gaaggugagc     2280

cucgaggacc ugcccggcuc ccccgcccug cggcgcagca ucaaccaggc caugcgggug     2340

gucgacgaga ucguguccau cgcgaagugc ccgcccagcc acaucuaccu cgaggugacc     2400

cgcgacgagg acggcgcccg caaggggcgg cgcuccaccc ggcgcuacga cgccaucaag     2460

gccgcccugg agaaguucaa ggaggagagc cccacguggu ucgaccagga cgucuucggc     2520

gaguuccggc gcagccgggc ggaggaccug gacgagcgcc ucucccugua cuucaugcag     2580

ggcggcaaga gccuguacuc cgccacgccc cucaagcugg agcugcucca caccaaccag     2640

uaccaggugg accacaucau cccgcagagc uacaucaagg acgacuccuu cgagaacaag     2700

gcccuggugc uggccgggga gaaccagcgc aagagcgacg cccuccugau cgacuccagc     2760

auccggaccg cgauggcccc cuacuggcgg gcccugcacc aggccgggcu caugggcgac     2820

aagaaguuca acucccugau gugcagcucc aucagcgaca agcgcguccg gggguucauc     2880

gcgcgccagc ugguggagac cucccagauc gugaagcucg ccggcaugau gcugcagagc     2940

aacuaccccg aggccgacgu ccagccggug aaggccuccc uggggcacga ccuccgggug     3000

caggccggcu ucaucaagug ccgcgagguc aacgacuucc accacgcgca cgacgcccug     3060

aucgccugcg agguggggcg guacaucaac cagugccacc ccggcaucua cgacaacccc     3120

aucggcuacg cccgcgccgu gcgggagcug guccgcaagc agaacgagga gaugcgcaag     3180

uacgggcggc uccccggcag cgcgggguuc aucgugggcg gguucaugcg cacccacgug     3240

gacgucgaca ccggcgaggu ggugugggac gccgaggagg agcugucccg gauccgcgcc     3300

uaccuggacu acagcaacgu cuucgugacc cggauguccg aggagacgag cggcgcccuc     3360

uggaacgcca ccaucuacag cccgcacuac gggaagaccg cgcccacccu gcacaucaag     3420

cagggccugg acgugcagcg cuacgggggc uacuccagcg ccaccuucgc cuacuucacg     3480

cucuacgucg ccgagaaggg caagaagcgg aaggccgagc ugcuggggau ccccaucucc     3540

guggcgcgca agaucgaggg cgagcccagc aagcucgacg ccuucguggc ccaggacgcc     3600

gaggcgaaga agcugaagca cccgcugauc auccgggccc gcauccucaa guacgcccgg     3660

uucgagaugg aggggaacca guucuacuuc gccgcccagg acgcggucua cuccgcccgc     3720

cagcuggugc ugaagggcga ccucuacagc guggccgcca aggccugcga caagcaccug     3780

aacgacuccu gcaucagcaa cgaggaccug auccggcucu acgacgagcu gguccacaag     3840

ggggaccugc ucugcaacaa guucggcaac gugaccgacg ugcugcgcgc gggccgggag     3900

cgcuacgccg ggcuguccau cagcgagaag cggcagcucg ucugcaacgu gcuggccuac     3960

uaccgcuccg ccaccgcccg gguggacauc agccuggucg gcgggcccaa gcacgcgggc     4020

aaccucggga ucgccuucgu gcugaacaac cccgaggcca ucguguucgu cgaccagucc     4080

gugaccggca uguucgagaa gcgcacgcgg guggagcugu ga                        4122


<210>  2003
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2003
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaca acgagcgcga gaaguccagc aucaucaaga ucgggguccg ggugaacccc      120

cucaccgugg acgagcagca gaacuucgag aagggcaagu ccaucacgac caacgcggac      180

cgcacccuga agcggagcau gcgccggaac cugcagcgcu ucaagcuccg gaaggagaac      240

cugaucgaga uccugaagaa gcagaaguuc aucaacgacg agaccauccu cuccgagaac      300

gggaacaaga gcacguucga gaccuacaac cugcgcgcca aguccgccac cgaggagauc      360

acccuggagc aguucgcccg gguccuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagagcca ggacgagggc cagcugaucg acgggaugga gaucgcgaag      480

aagcucuaca acgacaaccu gacgccgggc caguucgugu uccagauccu gaaggagggg      540

aagaaguaca uccccgacuu cuaccgcucc gaccuccagg ccgaguucga caagaucugg      600

agcuuccaga agcaguucaa ccccgacauc cugaccgagg aguucaacaa gcagaucauc      660

ggcaagggga aggugaacac caccaagauc uuccuggcca aguacggcau cuacacgauc      720

gacaacaagg gcgccgacaa gaagucccag gcguacaacu ggcgggucga ggcccucacc      780

aaccagcuga ccaaggagga gcuggccuuc gugaucagcg accucaacgg gaucaucucc      840

aacagcuccg gcuaccuggg gagcaucucc gaccgcagca aggagcugua cuucaacaag      900

cagaccgugg gccaguaccu caucgcccag cugaaggaga acccccacac gcagcugaag      960

aaccaggucu ucuaccggca ggacuaccuc gacgaguuca acaccaucug ggagacccag     1020

gccaaguucc acaaggagcu gaccaacgag cugaaggagg agauccgcga caucaucauc     1080

uucuaccagc ggaagcucaa gagccagaag ggccugaucu ccuucugcga guucgagagc     1140

aagcagaucg agaucaacau cgacgggaag aagaagcuga agacgguggg cuccaaggug     1200

ugccccaaga gcagcccccu cuuccagguc uucaagaucu ggcagauccu gaacaacauc     1260

caggugaucg agaaguccac cgggaagucc aaguuccugg agcaggacga gaaggagauc     1320

cucuucaagg agcugaacuu caaggacaag aucagcaaga ccgacgcgcu gaagauccuc     1380

uacaagaacu acaaggagcu ggacuucaac uucaaggaga acaucgaggg caaccgcacc     1440

caggccacgu uccugaaggc cuuccagucc aucaucgagc ucagcgggca cgaggugucc     1500

gacuucagca agaucaagac cgacgagauc cuggagaucg ucacccccau cuucgagggc     1560

uucggcuuca acaccaaccu gcuccaguuc aacuccaacc ugaaggggaa ggacuucgag     1620

aagcaggccg uguaccagcu guggcaccuc cucuacagcu ucgagggcga caacuccaac     1680

acggggaacg agaagcugau ccagaagauc agcgagcugu acggcuucga gcgggaguac     1740

gccaucauca ucgcgaacau cuccuuccag cccgacuacg ggagccucuc caccaaggcc     1800

auccgcaaga uccugccgca ccugaaggag ggcaucgagu ucagccucgc cugcgaguac     1860

gccggcuacc ggcacagcaa gaacucccug accaaggagg agaagaacaa gaagauccug     1920

aaggacaaga ucgacauccu ccccaagaac agccugcgca accccguggu cgagaagauc     1980

cugaaccaga ugaucaacgu ggugaacgcc accaucgaga cguacgggaa gcccgacgag     2040

auccgcaucg agcucgcgcg ggagcugaag aaguccgcca aggagcgcga ggaccugacc     2100

aacgccauca acaagagcac ccucgaccac gagaaguaca aggcccugcu gcagaaggag     2160

uucggccucu ccaacgucag ccggaacgac aucauccgcu acaagcugua ccuggagcuc     2220

gagcacaacg gguuccacac ccuguacucc aacacguaca ucgagccgaa caagcuguuc     2280

agcaaggagu ucgacaucga gcacaucauc ccccaguccc ggcuguucga cgacagcuuc     2340

uccaacaaga ccaucgagag ccgcuccgcc aacaucgaga agggcaacga gaccgcguuc     2400

gacuacguga gcaacaagaa cggggaggug ggccugaagg acuaccugaa gaacaucgag     2460

gaccucuuca acaacggcaa gaucuccaag accaaguaca acaagcugaa gaugaagggg     2520

gacgagaucc ccagcgacuu caucgcccgg gaccugcgcg acacccagua caucuccaag     2580

aaggccaaga gcauccucga ggaccugguc aaggacgugg ucagcacggg cggguccauc     2640

accgaccggc ugcgcgagga cuggcagcuc aucgacauca ugaaggagcu gaacugggac     2700

aaguacgaca agcugggccu caccgaggug aucgagaaca acgacggcca caagaucaac     2760

cggaucaagg acuggaccaa gcgcaacgac caccggcacc acgccaugga cgcgcugacg     2820

aucgccuuca ccaagcgcag cuacauccag uaccugaaca accucaacgc ccgguccgac     2880

aagagcgggu ccaucuacgg caucgagcag aaggagcugu accgcgacaa gaacaacaag     2940

cugcgguuca agcccccgau cccccucgac gacuuccgcg ccgaggccaa gaagcagcug     3000

gagaacaccc uggugagcau caaggcgaag aacaaggucg ccaccaucaa caucaacaac     3060

acgaagaagc agggcgggga gaacaagaag aagcagcuca ccccgcgggg gcagcugcac     3120

cuggagacca ucuacggcuc cauccagcag uacgugacca aggaggagaa ggugggcgcc     3180

aacuucaaca aggagcucau cgagaagguc gccaacaaga agugccgcga ggcccugcug     3240

aagcgccuca ucgaguucga caacgacccc aagaaggcgu ucacgcggaa gaacagccug     3300

aacaagaacc cgcuguacau cgacaccauc aagaccuaca aggugcccga gaaggugaag     3360

accgucacga ccgagaccau cuacaccauc cgcaaggaca ucaaccccga ccucaagauc     3420

gacaaggugg uggacaagaa gaucaagaac auccuggagc ugcggcucaa ggaguucaac     3480

ggggacgcca agaaggccuu cuccaaccug gacgagaagc ccaucuggcu gaacaaggag     3540

aagggcauca gcaucaagcg cgucacgauc accgggaucu ccaacgccau cgcccuccac     3600

gacaagaagg acaaggaggg caacuucauc cuggacaacc aggggaagaa gcaggcggug     3660

gacuucguga acaccggcaa caaccaccac gucgccaucu acaaggacga gaagggcaac     3720

cugcaggaga acgugaucac cuucuacgag gccguggagc gggucaacca ggggaucccg     3780

aucaucgaca agacguacaa gcagaacgag ggcuggcagu uccucuucag caugaagcag     3840

aacgaguacu ucguguuccc caacgagaag accggguuca acuccaacga gaucgaccug     3900

cugaaccccg agaacuacca ccucaucagc cccaaccugu uccgcgugca gaagaucgcc     3960

accaaggacu acuucuuccg gcaccaccug gagaccaacg ucgagaacau cacgaccacc     4020

aaggaccuca ccuggaagcg ccugggcauc aacgggauca ucaacauccu gaaggugcgg     4080

aucaaccaca ucggccagau cguguccguc ggcgaguacu ga                        4122


<210>  2004
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2004
augaccaaga agaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucacgg acgacuacaa ggugcccgcc aagaagauga aggugcucgg gaacaccgac      120

aagaaguaca ucaagaagaa ccugcugggc gcgcuccugu ucgacagcgg ggagaccgcc      180

gaggccaccc gccugaagcg gacggcccgc cggcgcuaca cccggcgcaa gaaccggcuc      240

cgcuaccugc aggagaucuu cgccgaggag augaccaagg ucgacgaguc cuucuuccag      300

cggcuggacg agagcuuccu ccgcugggac gacgacaaca agaagcuggg ccgguacccg      360

aucuucggca acaaggcgga cguggugaag uaccaccagg aguuccccac caucuaccac      420

cuccgcaagc accucgccga cuccagcgag aaggccgacc ugcggcuggu cuaccucgcc      480

cuggcgcaca ugaucaaguu ccgcgggcac uuccugaucg agggcgagcu caacgccgag      540

aacacggacg ugcagaagau cuucgccgac uucguggggg ucuacgaccg gaccuucgac      600

gacucccacc ugagcgagau caccguggac gccgccucca uccugaccga gaagaucagc      660

aagucccgcc ggcucgagaa ccugaucaag uacuacccca cggagaagaa gaacacccug      720

uucggcaacc ucaucgcgcu ggcccugggg cuccagccca acuucaagau gaacuucaag      780

cugagcgagg acgccaagcu gcaguucucc aaggacagcu acgaggagga ccucggcgag      840

cugcugggca agaucgggga cgacuacgcc gaccucuuca ccuccgccaa gaaccuguac      900

gacgcgaucc ugcucagcgg cauccugauc guggacgaca acuccaccaa ggccccgcug      960

agcgccucca ugaucaagcg cuacgucgag caccaggagg accucgagaa gcugaaggag     1020

uucaucaagg ccaacaagag cgagcuguac cacgacaucu ucaaggacaa gaacaagaac     1080

ggguacgccg gcuacaucga gaacggcgug aagcaggacg aguucuacaa guaccucaag     1140

aacacgcuga gcaagaucgc gggguccgac uacuuccugg acaagaucga gcgggaggac     1200

uuccuccgca agcagcggac cuucgacaac ggcagcaucc cccaccagau ccaccuccag     1260

gagaugcacg ccauccugcg ccgccagggg gacuacuacc ccuuccugaa ggagaaccag     1320

gaccggaucg agaagauccu gaccuuccgc auccccuacu acgugggccc gcuggcccgg     1380

aaggacuccc gcuucagcug ggccgaguac cacuccgacg agaagaucac ccccuggaac     1440

uucgacaagg ucaucgacaa ggagaagucc gccgagaagu ucaucacgcg gaugacccug     1500

aacgaccucu accugcccga ggagaaggug cugcccaagc acagccacgu guacgagacc     1560

uacgcggucu acaacgagcu caccaagauc aaguacguga acgagcaggg gaaggacagc     1620

uucuucgacu ccaacaugaa gcaggagauc uucgaccacg uguucaagga gaaccgcaag     1680

gucacgaagg agaagcugcu gaacuaccuc aacaaggagu ucccggagua ccggaucaag     1740

gaccugaucg gccuggacaa ggagaacaag agcuucaacg ccucccucgg caccuaccac     1800

gaccugaaga agauccugga caaggccuuc cucgacgaca aggugaacga ggaggugauc     1860

gaggacauca ucaagacccu gacccuguuc gaggacaagg acaugaucca cgagcgccuc     1920

cagaaguaca gcgacaucuu cacggccgac cagcugaaga agcuggagcg gcgccacuac     1980

accggguggg gccggcucuc cuacaagcug aucaacggga uccgcaacaa ggagaacaac     2040

aagaccaucc uggacuaccu caucgacgac ggcagcgcga accggaacuu caugcagcug     2100

aucaacgacg acacccugcc cuucaagcag aucauccaga agucccaggu cgugggggac     2160

gucgacgaca ucgaggccgu ggugcacgac cuccccggca gccccgccau caagaagggc     2220

auccugcagu ccgucaagau cguggacgag cuggugaagg ucauggggga caacccggac     2280

aacaucguga ucgagauggc ccgcgagaac cagacgacca accggggccg cagccagucc     2340

cagcagcggc ucaagaagcu gcagaacagc cugaaggagc ucgggagcaa cauccugaac     2400

gaggagaagc ccuccuacau cgaggacaag guggagaaca gccaccucca gaacgaccag     2460

cuguuccugu acuacaucca gaacggcaag gacauguaca ccggggacga gcuggacauc     2520

gaccaccugu ccgacuacga caucgaccac aucauccccc aggccuucau caaggacgac     2580

agcaucgaca accgcguccu gaccuccagc gcgaagaacc ggggcaaguc cgacgacgug     2640

cccagccugg acaucgugcg cgcccgcaag gccgaguggg uccggcucua caaguccggc     2700

cugaucagca agcgcaaguu cgacaaccug acgaaggccg agcgcggggg ccucaccgag     2760

gccgacaagg cgggguucau caagcggcag cugguggaga cccggcagau caccaagcac     2820

guggcccaga uccuggacgc ccgcuucaac accgaguccg acgagaacga caaggucauc     2880

cgggacguga aggugaucac gcucaagagc aaccuggucu cccaguuccg caaggacuuc     2940

gaguucuaca aggugcggga gaucaacgac uaccaccacg cccacgacgc cuaccugaac     3000

gcgguggucg gcaccgcccu ccugaagaag uacccgaagc uggccagcga guucguguac     3060

ggcgaguaca agaaguacga cauccgcaag uucaucacca acuccagcga caaggccacc     3120

gcgaaguacu ucuucuacuc caaccucaug aacuucuuca agcgggugau ccgcuacagc     3180

aacgggaagg ucaucgugcg gcccgugguc gaguacagca aggacacgga ggacaucgcc     3240

ugggacaaga aguccaacuu ccgcaccauc ugcaaggugc ugagcuaccc ccaggugaac     3300

aucgucaaga agguggagac ccagaccggc ggguucucca aggagagcau ccugcccaag     3360

ggcgacuccg acaagcucau cccgcggaag acgaagaagg ccuacuggga caccaagaag     3420

uacgggggcu ucgacagccc caccguggcc uacuccgucu ucgugguggc cgacgucgag     3480

aagggcaagg cgaagaagcu gaagaccgug aaggagcugg uggggaucag caucauggag     3540

cgcuccuucu ucgaggagaa ccccgucgag uuccucgaga acaagggcua ccacaacauc     3600

cgggaggaca agcugaucaa gcugcccaag uacagccucu ucgaguucga gggcgggaag     3660

cgccggcugc uggccuccgc cagcgagcuc cagaagggga acgagauggu gaucccgggc     3720

caccugguga agcugcucua ccacgcccag cgcaucaacu ccuucaacag cacgaaguac     3780

cuggacuacg ucuccgccca caagaaggag uucgagaagg ugcugagcug cguggaggac     3840

uucgcgaacc ucuacgucga cguggagaag aaccugucca agauccgggc cguggccgac     3900

agcauggaca acuucuccau cgaggagauc agcaacagcu ucaucaaccu gcucacccug     3960

accgcccugg gcgcccccgc ggacuucaac uuccucgggg agaagauccc ccgcaagcgc     4020

uacaccucca cgaaggagug ccugaacgcc acccugaucc accagagcau caccggccuc     4080

uacgagaccc ggaucgaccu guccaagauc ggggaggagu ga                        4122


<210>  2005
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2005
auggagaaca agaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucaccg acgacuacaa ggugcccagc aagaagauga aggugcucgg gaacacggac      120

aagcgcuuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcg      180

gaggcccggc gccugaagcg gaccgcccgc cggcgcuaca cgcggcgcaa gaaccggcug      240

cgcuaccucc aggagaucuu cgccgaggag auguccaagg ucgacagcuc cuucuuccac      300

cggcuggacg acagcuuccu gaucccggag gacaagaagg gcuccaagua ccccaucuuc      360

gccacccuca ucgaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg cggacagcaa ggagaagacc gaccuccggc ugaucuaccu ggcccucgcc      480

cacaugauca aguaccgcgg ccacuuccug uacgaggaca cguucgacau caagaacaac      540

gacauccaga agaucuucaa cgaguucauc uccaucuaca acaacaccuu cgaggggaac      600

agccuguccg gccagaacgu ccagguggag gccaucuuca ccgacaagau cagcaagucc      660

gcgaagcggg agcgcgugcu caagcuguuc cccgacgaga aguccaccgg gcuguucagc      720

gaguuccuca agcugaucgu gggcaaccag gccgacuuca agaagcacuu cgaccuggag      780

gagaaggccc cgcuccaguu cagccgggac acguacgacg aggaccugga gaaccugcuc      840

gggcagaucg gcgacgacuu cgccgaccug uucguggccg cgaagaagcu guacgacgcc      900

auccuccugu ccggcauccu gaccgucacc gaccccagca ccaaggcgcc ccucuccgcc      960

agcaugaucg agcgcuacga gaaccaccag aaggaccugg ccacgcugaa gcaguucauc     1020

aagaccaacc uccccgagaa guacgacgag guguucagcg accaguccaa ggacggguac     1080

gcgggcuaca ucgacgggaa gaccacccag gagagcuucu acaaguacau caagaaccug     1140

cuguccaagu ucgagggcgc cgacuacuuc cucgagaaga ucgagcggga ggacuuccug     1200

cgcaagcagc ggacguucga caacggcagc aucccgcacc agauccaccu ccaggagaug     1260

aacgccaucc ugcgccgcca gggggagcac uaccccuucc ugaaggagaa caaggagaag     1320

aucgagaaga uccugaccuu ccggaucccc uacuacgugg gcccccucgc ccgcgggaac     1380

cgggacuucg ccuggcugac ccgcaacucc gaccaggcga uccggccgug gaacuucgag     1440

gagaucgucg acaaggccag cuccgccgag agcuucauca acaagaugac caacuacgac     1500

cuguaccucc ccgaggagaa ggugcugccc aagcacuccc ugcuguacga gacguucgcc     1560

guguacaacg agcucaccaa ggucaaguuc aucgccgagg gccugcgcga cuaccaguuc     1620

cuggacagcc ggcagaagaa ggacaucuuc uacacccucu ucaaggcgga ggacaagcgc     1680

aaggugaccg agaaggacau cauccaguac cugcacacgg uggacgggua cgacggcauc     1740

gagcugaagg gcaucgagaa gcaguucaac gccucccuca gcaccuacca cgaccugcug     1800

aagaucauca aggacaagga guucauggac gaccccaaca acgaggagau ccucgagaac     1860

aucguccaca cccugaccau cuucgaggac cgggagauga ucaagcagcg ccuggcccag     1920

uacgacuccc ucuucgacga gaaggugauc aaggcccuga cgcggcgcca cuacaccggg     1980

uggggcaagc ugagcuccaa gcucaucaac gggauccggg acaagcagac cggcaagacc     2040

auccuggacu accugaugga cgacggguac aacaaccgca acuucaugca gcucaucaac     2100

gacgacgagc ugagcuucaa ggagaucauc aagaaggccc agguggucgg caagacggac     2160

gacgugaagc aggucgugca ggagcugccg ggcucccccg ccaucaagaa ggggauccuc     2220

cagagcauca agcuggugga cgagcugguc aaggugaugg gccacgagcc cgaguccauc     2280

gugaucgaga uggcccggga gaaccagacc accgcccgcg ggaagaagaa cagccagcag     2340

cgguacaagc gcaucgagga cagccucaag auccuggccu ccggccugaa cgcgaagauc     2400

cucaaggagc accccaccga caacauccag cugcagaacg accggcuguu ccucuacuac     2460

cugcagaacg ggcgcgacau guacaccggc aagccgcugg acaucaacca gcucagcucc     2520

uacgacaucg accacaucgu cccccaggcc uucaucaagg acgacagccu ggacaaccgc     2580

gugcugacgu cccucaagga caaccggggc aagagcgaca acgugcccuc ccuggagguc     2640

guggagaaga ugaagaccuu cuggcagcag cugcucgaca gcaagcugau cuccuaccgc     2700

aaguucaaca accugaccaa ggccgagcgc gggggccucg acgagcggga caaggugggg     2760

uucaucaagc ggcagcuggu cgagacccgc cagaucacga agcacguggc ccagauccug     2820

gacgcccggu acaacaccga ggugaacgag aaggacaaga agaaccgcac cgucaagauc     2880

aucacccuca agagcaaccu gguguccaac uuccggaagg aguuccgccu guacaagauc     2940

cgggagauca acgacuacca ccacgcgcac gacgccuacc ucaacgccgu ggucgccaag     3000

gccauccuga agaaguaccc caagcuggag ccggaguucg uguacggcga cuaccagaag     3060

uacgaccuca agcgcuacau cagccggucc aaggacccca aggagaucga gaaggcgacg     3120

gagaaguacu ucuucuacag caaccugcug aacuucuuca aggaggaggu gcacuacgcc     3180

gacggcacca ucgucaagcg cgagaacauc gaguacucca aggacaccgg ggagaucgcc     3240

uggaacaagg agaaggacuu cgccaccauc aagaaggugc ucagccugcc ccaggugaac     3300

aucgucaaga agcgggaggu gcagacgggc ggguucucca aggagagcau ccugcccaag     3360

ggcaacagcg acaagcucau cccgcgcaag accaaggaca uccuguggga caccaccaag     3420

uacgggggcu ucgacucccc cgugaucgcg uacagcaucc ugcucaucgc cgacaucgag     3480

aagggcaagg ccaagcggcu gaagacgguc aagacccugg uggggaucac caucauggag     3540

aaggccaccu ucgagaaguc ccccaucgcc uuccucgaga acaagggcua ccacaacgug     3600

cgcaaggaga acauccugug ccugccgaag uacagccucu ucgagcugaa gaacgggcgg     3660

cgccggaugc uggcguccgc caaggagcuc cagaagggca acgagaucgu ccugcccgug     3720

caccugacga cccuccugua ccacgccaag aacauccacc gccuggacga gcccgagcac     3780

cucgaguaca uccagaagca ccgcaacgag uucaaggggc ugcugaaccu cgugagcgag     3840

uucucccaga aguacguccu ggccgacgcc aaccuggaga agaucaagaa ccucuacgcg     3900

gacaacgagc aggccgacau cgagauccug gccaacagcu ucaucaaccu gcucaccuuc     3960

accgcccugg gcgcccccgc ggccuucaag uucuucggca aggacgugga ccggaagcgc     4020

uacacgaccg uguccgagau ccugaacgcc acccucaucc accagagcau caccgggcug     4080

uacgagacgc ggaucgaccu guccaagcuc ggcgaggacu ga                        4122


<210>  2006
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2006
auggagacca aguucaccua cuaccugggc cucgacaugg ggacggacag ccugggcugg       60

gccgugaccg acuccagcua ccacauccug cgcgccaagg ggaaggaccu cuggggcguc      120

cggcuguucc ccuccgcgga gaccagcgug gagcgccgga ccuaccgcgu gaaccggcgc      180

cggcgccagc gggaggucgc ccgcaucggg gcccugaagg agcuguucgc cgacgagauc      240

gagaaggugg acccgggcuu cuucgugcgg cuggacgagu ccaaguucca cauggaggac      300

cgcagcgaga acaacaagca gaaguacggc cuguucgccg acaagcuguu cuccgacgcg      360

ggguacuaca agaaguaccc cacgaucuuc caccuccgga aggagcuccu cgagagcgag      420

gagccccacg acguccgccu gguguaccug gcccuccuga acauguucaa gcaccggggc      480

cacuuccuga acuccagccu ccagaucgag ggggagaacu ccaacaucgc cgagagcuac      540

cagauguucg uggacacccu gaccgacgag gaccuggacu uccccgagca ggucuccggc      600

gagcgccucc aggagaucuu ccggagcucc gacagccgca agaagaaggu ggagaaccug      660

cuggccgaga ugaaccucuc caagaagcag aagcgggaga uccaguucgu gaaccugauc      720

ugcgggcuga agggcaagcu cagcgagcug uucccggaca aggaguacga cgaggagcag      780

aagaagaucg cguucuccuu ccaggacagc gacuacgagg acaaggagaa caccauccag      840

gagcugcucg acgagagcga gaugaacaug aucuacgccg ucaaggccgu gcacgacucc      900

ggcgugcuga gcgggaucau gaacggcgag cgcuaccugu ccgucgcccg gguggccagc      960

uacgagaagc acaagaagga ccuccagauc cugaaggaca uccugaagaa guacgacacg     1020

aaggaguacu ucgagauguu ccgcaacgag uccgccaaga gcgaguccua cagcgcguac     1080

gugggguccg ucaaguacca cggcaccuuc cgccggggcc ucagcggggg caagcaggag     1140

gaccuguaca agcacaucaa gaaggugcug gggaaguucc cccaggacga cgagggcgug     1200

accuacaucc ucgaggagau cgagaccgag uccuuccugc ccaagcagcu gacggccagc     1260

aacgggguca uccccaacca ggugcacgcc cgcgagaugc ucgccauccu gaagcacgcg     1320

gagcuguacc ucccguuccu gcaggagaag gacgacaccg gccuguccgu gagcgagaag     1380

auccucaagc uguucauguu ccagaucccc uacuacgucg gcccccuggg gcaggaguac     1440

aaggacaaga agggcuacaa cgugugggcc gagcggaagg ccuccgggaa gaucuacccc     1500

uggaacuucg aggacaagau cgacgucaag aaguccgccg agaaguucau cggccgcaug     1560

gugcggcgcu gcaccuaccu gagcggggag caggcccucc cgaagcagag ccugcuguac     1620

gagaaguucc aggugcucaa cgagcugaac aaccugcgga uccgcggcga gaagaucccc     1680

gucucccuca agcaggagau cuaccacgac cuguucggcg acgggaagaa ggugagcaug     1740

acccggcuga cgaacuaccu ccugcagaac ggcgugauca gcggggagga gaaggacucc     1800

ugcaucagcg gcaucgacgg gggcuuccac aacucccuga gcuccgucgg caaguucaag     1860

gggguguucg gcgacaccgu gcacaccgac gagcaccgca agaugaucga ggacaucauc     1920

uucuggggga ccgucuucgg cgacggcaag aagcuccuga aggagaaggu ggaggaggug     1980

uacgggaacc agcugacgga ggcgcagaag aagcggaucu ucggcuucaa guucagcucc     2040

ugggggcgcc ucagcaaggc cuuccugacc auggagggcg ccuccaagga cgacggggag     2100

aucaagggcc ugauccaggc caugugggac accaacugca accucaugga gcugcugagc     2160

gaccgguuca ccuacaagga caagcucaac gagaugguca cgaccgccga gaagccccug     2220

ugcgagugga agaucgagga ccuggacgag auguaccucu ccgcgcccgu gaagcgcaug     2280

guguggcaga ccauccgggu cguggacgag gugcagcaga ucaucggcug cccgcccaag     2340

cgcaucuucg ucgagaugac ccggagcgac ggggagaagg gcaagcgcac ggugucccgg     2400

cagaagaagc ugguggaccu guacaaggcc cucgggaagg agggcaagac cuggagcgag     2460

gagcuguccg acacccccga gagccaguuc cgcaucaaga agcuguaccu cuacuaccug     2520

cagaugggga agugcaugua caccggcgag gccaucgagc uggagcagcu caugaacgac     2580

aaccuguacg acaucgacca caucuacccc cgccacuuca ucaaggacga caacaucucc     2640

aacaaccugg uccucgugaa gaaggagaag aacgcccaca agagcgacag cuucccgauc     2700

gagcccgaga uccggaacaa gaugaugccc uucuggaagu cccugcgcaa gaagggcuuc     2760

cugacggagg agaaguacaa ccggcucacc cgcaugagcg cguucaccga cgaggagaag     2820

gccgccuuca ucgggcggca gcugguggag accucccagg gcacgaaggc caucacccag     2880

aucuucgagc aggccuucgc gaacagcgag aucgucuucu ccaaggccag cguggugucc     2940

gccuuccgcc agaaguucga cugcuacaag guccggugcg ugaacaacua ccaccacgcc     3000

cacgacgccu accugaacau cguggcgggg aacaccuacc gcgucaaguu caccagcaac     3060

ccccucaacu ucaucaagga ggccaagaag aacccgaacu cccgggagaa ccgcuacaac     3120

auggacaaga ucuucgagug ggacgugacc cggaacggcg agacggccug gguggcccag     3180

accaaggagc accccgggac cauccagcug gucaagaagu accugaacaa gaacagcccc     3240

cucaucacca agauggugua cgagaagcac ggcggcauca cgcagaaggc caccaucugg     3300

uccgcgaaga aggccgagag cgggaacacc gacgccuaca uccccgugaa gaugaacgac     3360

ccgcgccugg ccgacgucac caaguacggc ggggugacga ccaucgcggu ggccggcuac     3420

acccugcucg aguacaaggc cgggggcaag accguccggu cccuggaggc ccugcccauc     3480

uaccucggcg ggagcaagaa gcugacggag gagcagaugg uggaguacuu cuccaacgug     3540

cugcagcagg agaacaagga gaaggucgug agcgacguga ucauccgcaa gaagaugauc     3600

cccuccggca gccucaucaa guacaacggg uucuacuacu accuggccgg caagaccggc     3660

gaccggauca uccugaucuc cgcgguccag cucugccugc cgauggacac caugcuguac     3720

aucaagaaga ucgagaaggc cugcagcacc gggaacuucg aggagaagga caagaacggc     3780

aagcaggucc ucagcaagca gcagaacgag uucguguacg agcugaucau ccagaaguac     3840

cagcagucca ucuucaagaa gaaggugggg gccaucggcg acacgaucau cgagaagcgc     3900

gagaaguucg ccgagaucag ccuggaggag cagugcaagg ugcucaugca gaucuuccug     3960

aacuuccagu ccgggaucgg cguggagcug aagaucaucg agggcgggaa gaaguccggc     4020

gucaccuacc ucaacaagaa ggugaugaac guccaggagc ugaagcugau cgaccagagc     4080

gugaccgggg uguucagccg ggagaucgac cuccugacca ucuga                     4125


<210>  2007
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2007
augaagaagc gcuacagcua cuccaucggc cuggacaucg ggaccaacag cgugggcugg       60

gccgucauca acgaggacua caaggugccc gccaagaaga ugaccguguu cgggaacacg      120

gaccggaaga ccaucaagaa gaaccuccug ggcaccgucc uguucgacuc cggggagacc      180

gcgcaggccc gccggcucaa gcgcacgaac cggcgccggu acacccgccg gcgcuaccgg      240

cugugccagc ugcagaacau cuucgccacc gagaugguga agguggacga caccuucuuc      300

cagcgccuca gcgaguccuu cuucuacuac caggacaagg ccuucgacaa gcacccgauc      360

uucggcaaca gcaaggagga gcgggccuac cacaagacgu accccaccau cuaccaccuc      420

cgcaaggacc uggcggaccg ggaccagaag gccgaccucc gccugaucua ccuggcccuc      480

ucccacauca ucaaguuccg gggccacuuc cugaucgagg ggaagcugaa cagcgagaac      540

accgacgucc agaagcuguu caucgcccug gugaccgugu acaaccuccu cuucgaggag      600

gagcccaucg cgggcgagac gugcgacgcc aaggcccugc ugaccgccaa gaccuccaag      660

agcaagcgcc ucgagucccu gaucagcgag uuccccgggc agaagaagaa cggccuguuc      720

gggaaccucc uggcccuggc gcucggccug cggccgaacu ucaaguccaa cuucggccug      780

agcgaggacg ccaagcucca gaucaccaag gacacguacg aggaggagcu ggacaaccug      840

cucgccgaga ucggggacca cuacgccgac cuguuccugg ccgcgaagaa ccucuccgac      900

gccauccugc ugagcgacau ccucacccug uccgacgaga acacccgcgc gccccugagc      960

gccuccauga ucaagcggua cgaggagcac caggaggacc ucgcccugcu gaagaagcuc     1020

gucaaggagc agaugcccga gaaguacugg gagaucuuca gcaacgccaa gaagaacggc     1080

uacgccgggu acaucgaggg caaggugagc caggaggacu ucuaccgcua caucaagccc     1140

auccuguccc gccugaaggg cggggacgag uuccucgcca agaucgaccg ggacgacuuc     1200

cugcgcaagc agcggaccuu cgacaacggc agcaucccgc accagaucca ccugaaggag     1260

cugcacgcca uccuccgccg gcaggagaag uacuaccccu uccuggccga gcagaaggag     1320

aagaucgagc agcugcucug cuuccgcauc cccuacuacg uggggccccu ggcgaagggc     1380

gggaacucca gcuucgccug gcugaagcgg cgcuccgacg agccgaucac gcccuggaac     1440

uucaaggacg ucguggacga ggaggccagc gcccaggcgu ucaucgaggg caugaccaac     1500

uacgacaccu accuccccga ggagaaggug cugcccaagc acuccccgcu guacgagaug     1560

uucaccgucu acaacgagcu cacgaaggug aaguacaucg ccgagaacau gaccaagccc     1620

cuguaccuga gcgccgagca gaaggaggcc aucaucgacc accucuucaa gcagacccgg     1680

aaggugaccg ucaaggaccu gaaggagaag uacuucuccc agaucgaggg ccuggagaac     1740

guggacguga cgggggucga gggcgccuuc aacgcgagcc ucgggaccua caacgaccug     1800

cugaagauca ucaaggacaa ggccuuccuc gacgacgagg ccaacgccga gauccuggag     1860

gagaucgugc ugauccucac ccuguuccag gacgagaagc ugaucgagaa gcgccucgcc     1920

aaguacgcga accuguucga gaaguccgug cugaagaagc uccggaagcg ccacuaccgg     1980

ggcugggggc gccugagccg gcagcugauc gacggcauga aggacaaggc cuccggcaag     2040

accauccucg acuuccugaa ggccgacgac uucgccaacc gcaacuucau ccagcugauc     2100

aacgacagcu cccucgacuu cgagaagcug aucgacgacg cccagaagaa ggcgaucaag     2160

cgggagagcc ugacggaggc cgucgccaac cucgccgggu cccccgcgau caagaagggc     2220

auccugcaga gccugaaggu ggucgacgag aucgugaagg ugauggggca caaccccgac     2280

aacaucguca ucgagauguc ccgcgagaac cagaccaccg cccagggccu caagaacgcc     2340

cggcagcgcc ugaagaagau caaggaggug cacaagaaga ccgggagccg cauccuggag     2400

gacaacagcg agcggaucac gaaccucacc cugcaggaca accgccugua ccucuaccug     2460

cugcaggacg gcaaggacau guacaccggc caggaccucg acaucaacaa ccugucccag     2520

uacgacaucg accacaucau cccgcagagc uucaucaagg acaacuccau cgacaaccug     2580

gugcucacca cccagaaggc caaccggggc aagagcgaca acguccccuc caucgaggug     2640

gugcgcgaca ugaaggaccg ggucuggcgc cggcagcugg ccaacggggc gaucagccgc     2700

cagaaguucg accaccugac gaaggccgag cgcgggggcc ucgccgacuc cgacaaggcc     2760

cgguuccugc ggcgccagcu gguggagacc cggcagauca ccaagcacgu ggcccagcuc     2820

cuggacagcc gcuucaacuc caagagcaac cagaacaaga agcuggcgcg gaacgucaag     2880

aucaucaccc ucaaguccaa gaucgugagc gacuuccgca aggacuucgg ccuguacaag     2940

cugcgggagg ugaacaacua ccaccacgcc cacgacgccu accucaacgc cgucgugggg     3000

acggcccugc ugaagaagua ccccaagcuc gaggcggagu ucguguacgg cgacuacaag     3060

cacuucgacc uggucaagcu gaucuccaag agcgaccccu cccucgggaa ggccaccgcc     3120

aagguguucu ucuacagcaa caucaugaac uucuucaagg aggagcuguc ccuggccgac     3180

ggcacccuca ugaagcgccc ggugaucgag accaacacgg agaccgggga ggucgugugg     3240

gacaagguga aggacuucaa gaccauccgg aagguccuga gcuaccccca ggugaacauc     3300

gugaagaaga ccgagaucca gagcggcgcg uucuccaagg agagcguccu guccaagggc     3360

aacagcgaca agcucaucga gcgcaagaag gggugggacc ccaagaagua cggcggguuc     3420

gacuccccca acacggccua cagcaucuuc gugguggcca aggucgccaa gcggaaggcc     3480

cagaagcuga agaccgugaa ggagaucgug ggcaucacca ucauggagca ggcggaguac     3540

gagaaggaca acaucgccuu ccuggagaag aagggguacc aggacaucca ggagaagcuc     3600

cugaucaagc ugccgaagua cucccucuuc gagcuggaga acggccgccg ccggcugcuc     3660

gccagcgcca acgaguucca gaagggcaac gagcuggccc uguccgggaa guacaugaag     3720

uuccucuacc uggcgagccg cuacgacaag cuguccagca agaucgaguc cgagcagcag     3780

aagaagcugu ucgucgagca gcaccuccac uacuucgacg agauccugga caucguggug     3840

aagcacgcga ccugcuacau caaggccgag aacaaccuga agaagaucau cagccuguac     3900

aagaagaagg aggccuacuc caucaacgag caggcccuga acaugcugaa ccucuucauc     3960

uucacgagcc ugggcgcccc cuccaccuuc gucuucuucg acgagaccau cgaccggaag     4020

cgcuacacca cgagcuccga cgugcugaac gggauccuca uccagcagag caucaccggc     4080

cuguacgaga cccggaucga ccugagccgc uucgggggcg acuga                     4125


<210>  2008
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2008
augaagaagc agaaguucag cgacuacuac cugggcuucg acaucgggac caacuccgug       60

ggcuggugcg ucaccgaccu cgacuacaac gugcugcgcu ucaacaagaa ggacaugugg      120

gggagccggc uguucgacga ggccaagacg gccgcggagc gccgggugca gcgcaacucc      180

cggcgccggc ucaagcgccg gaaguggcgc cugaaccugc ucgaggagau cuucagcgac      240

gagaucauga agaucgacuc caacuucuuc cggcgccuga aggagagcuc ccuguggcuc      300

gaggacaaga acagcaagga gaaguucacc cuguucaacg acgacaacua caaggacuac      360

gacuucuaca agcaguaccc caccaucuuc caccuccggg acgagcucau caagaacccg      420

gagaagaagg acauccgccu gaucuaccug gcccuccacu ccaucuucaa gagccggggc      480

cacuuccugu ucgaggggca gaaccugaag gagaucaaga acuucgagac ccucuacaac      540

aaccugaucu ccuuccugga ggacaacggc aucaacaaga gcaucgacaa ggacaacauc      600

gagaagcucg agaagaucau cugcgacucc ggcaaggggc ugaaggacaa ggagaaggag      660

uucaagggca ucuucaacag cgacaagcag cuggucgcca ucuucaagcu cuccgugggg      720

agcuccguga gccugaacga ccuguucgac acggacgagu acaagaagga ggaggucgag      780

aaggagaaga ucuccuuccg cgagcagauc uacgaggacg acaagcccau cuacuacagc      840

auccucggcg agaagaucga gcugcuggac aucgccaaga gcuucuacga cuucauggug      900

cucaacaaca uccuguccga cagcaacuac aucuccgagg ccaaggugaa gcuguacgag      960

gagcacaaga aggaccucaa gaaccugaag uacaucaucc ggaaguacaa caaggagaac     1020

uacgacaagc uguucaagga caagaacgag aacaacuacc ccgcguacau cgggcucaac     1080

aaggagaagg acaagaagga ggucguggag aagucccgcc ugaagaucga cgaccugauc     1140

aaggugauca agggcuaccu ccccaagccg gagcggaucg aggagaagga caagaccauc     1200

uucaacgaga uccugaacaa gaucgagcug aagaccaucc uccccaagca gcgcaucagc     1260

gacaacggca cccugcccua ccagauccac gaggucgagc uggagaagau ccucgagaac     1320

cagagcaagu acuacgacuu ccugaacuac gaggagaacg ggguguccac gaaggacaag     1380

cugcucaaga ccuucaaguu ccggaucccc uacuacgugg gcccgcugaa cagcuaccac     1440

aaggacaagg gcgggaacuc cuggaucguc cgcaaggagg agggcaagau ccugcccugg     1500

aacuucgagc agaaggugga caucgagaag uccgccgagg aguucaucaa gcgcaugacc     1560

aacaagugca ccuaccugaa cggggaggac gugaucccca aggacagcuu ccucuacagc     1620

gaguacauca uccugaacga gcugaacaag guccagguga acgacgaguu ccucaacgag     1680

gagaacaagc ggaagaucau cgacgagcug uucaaggaga acaagaaggu guccgagaag     1740

aaguucaagg aguaccugcu cgucaaccag aucgccaacc gcacggugga gcugaagggc     1800

aucaaggaca gcuucaacuc caacuacgug agcuacauca aguucaagga caucuucggg     1860

gagaagcuga accucgacau cuacaaggag aucuccgaga aguccauccu guggaagugc     1920

cuguacggcg acgacaagaa gaucuucgag aagaagauca agaacgagua cggggacauc     1980

cucaacaagg acgagaucaa gaagaucaac agcuucaagu ucaacaccug gggccggcug     2040

agcgagaagc ugcucaccgg caucgaguuc aucaaccugg agaccgggga gugcuacagc     2100

uccgucaugg aggcccugcg ccggacgaac uacaaccuca uggagcugcu gagcuccaag     2160

uucacccucc aggagagcau cgacaacgag aacaaggaga ugaacgaggu guccuaccgc     2220

gaccugaucg aggagagcua cgucuccccc agccugaagc gggcgauccu ccagacccug     2280

aagaucuacg aggagaucaa gaagaucacc ggccgcgugc cgaagaaggu guucaucgag     2340

auggcccgcg ggggcgacga guccaugaag aacaagaaga uccccgcccg gcaggagcag     2400

cugaagaagc ucuacgacag cugcgggaac gacaucgcca acuucuccau cgacaucaag     2460

gagaugaaga acagccuguc cagcuacgac aacaacuccc ugcggcagaa gaagcucuac     2520

cuguacuacc ugcaguucgg caagugcaug uacacgggcc gcgagaucga ccucgaccgg     2580

cugcugcaga acaacgacac cuacgacauc gaccacaucu acccccgcag caaggucauc     2640

aaggacgacu ccuucgacaa ccucgugcug gugcugaaga acgagaacgc cgagaagucc     2700

aacgaguacc ccgucaagaa ggagauccag gagaagauga aguccuucug gcgguuccuc     2760

aaggagaaga acuucaucag cgacgagaag uacaagcgcc ugaccgggaa ggacgacuuc     2820

gagcugcggg gcuucauggc gcgccagcuc gugaacgugc ggcagaccac gaaggagguc     2880

gggaagaucc ugcagcagau cgagccggag aucaagaucg uguacagcaa ggccgagauc     2940

gccagcagcu uccgcgagau guucgacuuc aucaaggugc gcgagcugaa cgacacccac     3000

cacgccaagg acgccuaccu caacaucguc gcgggcaacg uguacaacac caaguucacc     3060

gagaagcccu accgguaccu gcaggagauc aaggagaacu acgacgugaa gaagaucuac     3120

aacuacgaca ucaagaacgc cugggacaag gagaacuccc uggagaucgu caagaagaac     3180

auggagaaga acacggugaa caucacccgc uucaucaagg aggagaaggg ggagcuguuc     3240

aaccugaacc ccaucaagaa gggcgagacc agcaacgaga ucaucuccau caagcccaag     3300

cuguacgacg gcaaggacaa caagcucaac gagaaguacg gguacuacac cagccugaag     3360

gccgccuacu ucaucuacgu ggagcacgag aagaagaaca agaaggucaa gacguucgag     3420

cggaucaccc gcaucgacuc cacccugauc aagaacgaga agaaccucau caaguaccug     3480

gugagccaga agaagcugcu caacccgaag aucaucaaga agaucuacaa ggagcagacc     3540

cugaucaucg acuccuaccc cuacacguuc accggcgugg acagcaacaa gaaggucgag     3600

cugaagaaca agaagcagcu cuaccuggag aagaaguacg agcagauccu gaagaacgcc     3660

cucaaguucg uggaggacaa ccagggggag accgaggaga acuacaaguu caucuaccug     3720

aagaagcgga acaacaacga gaagaacgag accaucgacg cggugaagga gcgcuacaac     3780

aucgaguuca acgagaugua cgacaaguuc cuggagaagc ucuccagcaa ggacuacaag     3840

aacuacauca acaacaagcu guacaccaac uuccugaacu ccaaggagaa guucaagaag     3900

cucaagcugu gggagaaguc ccugauccuc cgggaguucc ugaagaucuu caacaagaac     3960

acguacggca aguacgagau caaggacagc cagaccaagg agaagcuguu cagcuucccc     4020

gaggacaccg gccgcauccg gcucgggcag uccagccugg gcaacaacaa ggagcugcuc     4080

gaggagagcg ucaccgggcu guucgugaag aagaucaagc uguga                     4125


<210>  2009
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2009
augagcaaca agcccuacuc caucggccug gacaucggga ccaacagcgu gggcugggcc       60

gucaucaccg acgacuacaa ggugccgucc aagaagauga aggugcucgg gaacacggac      120

aagcacuuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcg      180

gaggaccgcc ggcugaagcg caccgcccgg cgccgguaca cgcgccggaa gaaccgccug      240

cgguaccucc aggagaucuu cuucgaggag augagcaagc uggacuccgg cuucuuccac      300

cgccuggacg acuucuuccu cauccccgag gacaagcggg gcagcaagua ccccaucuuc      360

gccacccugg ccgaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcucg ccgacuccaa ggagaaggcg gaccugcggc ugaucuaccu cacccuggcc      480

cacaugauca aguaccgcgg gcacuuccug uacgaggaga gcuucgacau caagaacaac      540

gacauccaga agaucuucaa cgaguucauc uccaucuacg acaacacguu cgagggcagc      600

ucccucagcg ggcagaacgc ccagaucgag gccaucuuca ccgacaagau cuccaagagc      660

gcgaagcggg agcgcguccu gaagcuguuc ccggacgaga aguccaccgg ccucuucagc      720

gaguuccuga agcugaucgu ggggaaccag gccgacuuca agaagcacuu cgagcucgag      780

gagaaggcgc cccugcaguu ccccaaggac accuacgacg aggaccugga gaacgugcuc      840

ggccagaucg gcgacgacuu cgccgaccug uucgucgccg ccaagaagcu guacgacgcc      900

auccuccugu ccgggauccu gacggugacc gaccccagca ccaaggcccc gcucuccgcc      960

agcaugaucg agcgguacga gaaccaccag aaggaccugg ccgcgcugaa gcaguucauc     1020

aagaccaacc uccccgagaa guacguggag gucuucagcg accaguccaa ggacggcuac     1080

gccggguaca ucgacggcaa gacgacccag gaggccuucu acaaguacau caagaaccug     1140

cugagcaagu ucgagggcac cgacuacuuc cucgacaaga ucgagcgcga ggacuuccug     1200

cggaagcagc gcaccuucga caacgggucc aucccccacc agauccaccu ccaggagaug     1260

aacgccaucc uccggcgcca gggcgagcac uaccccuucc ugaaggagaa caaggagaag     1320

aucgagaaga uccugacguu ccgcaucccg uacuacgugg ggccccucgc ccggggcaac     1380

cgcgacuucg cguggcugac ccggaacagc gaccaggcca uccgccccug gaacuucgag     1440

aagaucgugg acaaggccuc cagcgccgag gacuucauca acaagaugac caacuacgac     1500

cuguaccucc ccgaggagaa gguccugccg aagcacuccc ugcuguacga gaccuucgcc     1560

guguacaacg agcucacgaa ggugaaguuc aucgcggagg ggcugcggga cuaccaguuc     1620

cuggacagcg gccagaagaa gcagaucguc aaccagcugu ucaaggagaa gcgcaaggug     1680

accaagaagg acaucaucca cuaccugcac accguggacg gcuacgacgg gaucgagcug     1740

aagggcaucg agaagcaguu caacgccucc cucagcaccu accacgaccu gcugaagauc     1800

aucaaggaca agaaguucau ggacgacucc aagaacgagg ccauccucga gaacaucguc     1860

cacacgcuga ccaucuucga ggaccgggag augaucaagc agcgccuggc ccaguacgac     1920

agccucuucg acgagaaggu gaucaaggcg cugacccggc gccacuacac cggguggggc     1980

aagcuguccg ccaagcucau caacgggauc ugcgacaagc agacgggcaa caccauccug     2040

gacuaccuga ucgacgacgg cgagaucaac cggaacuuca ugcagcucau caacgacgac     2100

gggcugagcu ucaagaagau cauccagaag ucccaggugg ucggcgagac cgacgacgug     2160

aagcaggucg ugcgcgagcu gcccgggagc cccgccauca agaagggcau ccuccagucc     2220

aucaagaucg uggacgagcu ggucaaggug auggaccacg cccccgagag caucgugauc     2280

gagauggccc gggagaacca gaccacggcg cgcgggaaga agaacuccca gcagcgguac     2340

aagcgcaucg aggacagccu gaagaaccuc gccagcggcc uggacuccaa cauccugaag     2400

gagaacccga ccgacaacau ccagcucaag aacgaccggc uguuccugua cuaccuccag     2460

aacggcaagg acauguacac cggggaggcc cuggacauca accagcugag cuccuacgac     2520

aucgaccaca ucauccccca ggccuucauc aaggacgaca gccucgacaa ccgcguccug     2580

accuccagca aggacaaccg gggcaagucc gacaacgugc ccagccugga ggugguccag     2640

aagcgcaagg ccuucuggca gcagcuccug gacuccaagc ugaucagcga gcgcaaguuc     2700

aacaaccuca ccaaggcgga gcgcgggggc cugaacgagc gggacaaggu gggcuucauc     2760

aagcggcagc ugguggagac gcgccagauc accaagcacg ucgcccagau ccucgacgcc     2820

cgguacaaca ccgaggugaa cgagaaggac aagaagaacc gcaccgugaa gaucaucacg     2880

cugaagucca accuggucag caacuuccgg aaggaguucc gccucuacaa ggugcgggag     2940

aucaacgacu accaccacgc ccacgacgcc uaccugaacg cgguggucgc caaggccauc     3000

cugaagaagu accccaagcu cgagccggag uucguguacg gggaguacca gaaguacgac     3060

cugaagcgcu acaucucccg gagccgcaac cccaaggagg uggagaaggc caccgagaag     3120

uacuucuucu acuccaaccu gcucaacuuc uucaaggagg agguccacua cgcggacggc     3180

accaucguga agcgggagaa caucgaguac agcaaggaca ccggggagau cgccuggaac     3240

acggagaagg acuucgccac caucaagaag auccuguccu acccccaggu gaacaucguc     3300

aagaagaccg aggagcagac cgugggccag aacgggggcc uguucgacaa caacaucgug     3360

agcaaggaga aggucgugga cgccagcaag cucaucccca ucaaguccgg ccugagcccg     3420

aagaaguacg ggggcuacgc ccgccccacg aucgcguacu ccgugcuggu caccuacaag     3480

cagaacaaca agaucaagaa caccauggug gggauccccg ugcucaccaa gcuggaguuc     3540

gagcgggacc cgaaccugua ccuccgcaac cugggcauca acaacgucag ccacgugauc     3600

aagcugguga aguacacggu ccucgaguuc aagcacaagu acgggaccua ccggcgcuuc     3660

aucgugucca accaggagcu gaagcgggcc aaccagaucu ucgugaccac caagcagaug     3720

aagcugauca gccaguacac gacccucgac aagaacgagc acgacucccu gcugaagaac     3780

aaccagcagc ucauccagag caucuggaac caguuccugg aguucaugcu ggccaccgac     3840

cucgucaaca agaagcagua cgacgagcug auccugacca acaaccagac gaucgaggag     3900

ugccgcaaca ucaugaaccg ggugaucacc acccucuccu ucaccaacau caacgccggc     3960

cagaagaaga ucaucuucga cgacacgcug agccugccca ucaagcgcug gcaguucacc     4020

gacuccgaca agauggugaa gaagaucauc gagagcaccc ucauccacca guccaucacc     4080

ggccuguacg agacgcgcau cgaccugagc aagcucgggg aggacuga                  4128


<210>  2010
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2010
augaaggagu accacaucgg ccuggacauc gggaccagcu ccaucggcug ggccgugacc       60

gacagccagu ucaagcucau gcgcaucaag gggaagacgg ccaucggcgu ccggcuguuc      120

gaggagggga agaccgcggc cgagcgccgg accuuccgca ccacgcggcg ccggcugaag      180

cgccggaagu ggcgccucca cuaccuggac gagaucuucg ccccccaccu gcaggaggug      240

gacgagaacu uccuccggcg ccugaagcag uccaacaucc acccggagga ccccgccaag      300

aaccaggccu ucaucggcaa gcugcucuuc cccgaccugc ugaagaagaa cgagcggggc      360

uaccccaccc ucaucaagau gcgcgacgag cugccggugg agcagcgggc gcacuacccc      420

gucaccaaca ucuacaagcu gcgcgaggcc augaucaacg aggaccggca guucgaccuc      480

cgcgaggugu accuggccgu gcaccacauc gucaaguacc gggggcacuu ccugaacaac      540

gccagcgugg acaaguucaa ggugggccgc aucgacuucg acaaguccuu caacguccuc      600

aacgaggcgu acgaggagcu gcagaacggg gagggcagcu ucaccaucga gcccuccaag      660

guggagaaga ucgggcagcu gcuccuggac acgaagaugc ggaagcugga ccgccagaag      720

gccguggcca agcuccugga ggucaaggug gccgacaagg aggagaccaa gcgcaacaag      780

cagaucgcca ccgcgaugag caagcuggug cucggcuaca aggccgacuu cgccaccguc      840

gccaugguga acggcaacga guggaagauc gaccugucca gcgagacguc cgaggacgag      900

aucgagaagu uccgggagga gcugagcgac gcccagaacg acauccucac cgagaucacc      960

ucccuguuca gccagaucau gcugaacgag aucgugccca acgggauguc caucagcgag     1020

uccaugaugg accgcuacug gacccacgag cggcagcucg cggaggucaa ggaguaccug     1080

gccacgcagc cggccagcgc ccgcaaggag uucgaccagg uguacaacaa guacaucggc     1140

caggccccca aggagaaggg guucgaccug gagaagggcc ucaagaagau ccuguccaag     1200

aaggagaacu ggaaggagau cgacgagcug cucaaggcgg gcgacuuccu gcccaagcag     1260

cggaucagcg ccaacggggu gaucccccac cagaugcacc agcaggagcu ggaccgcauc     1320

aucgagaagc aggccaagua cuacccgugg cucgccaccg agaaccccgc caccggcgag     1380

cgggaccgcc accaggcgaa guacgagcug gaccagcugg ucagcuuccg gauccccuac     1440

uacguggggc cccucgugac cccggagguc cagaaggcca cguccggcgc caaguucgcc     1500

ugggcgaagc gcaaggagga cggggagauc acccccugga accuguggga caagaucgac     1560

cgggccgaga gcgccgaggc cuucaucaag cgcaugaccg ugaaggacac cuaccugcuc     1620

aacgaggacg uccugcccgc caacucccug cuguaccaga aguacaacgu gcucaacgag     1680

cugaacaacg ugcgggucaa cggccgccgg cugagcgugg gcaucaagca ggacaucuac     1740

acggagcucu ucaagaagaa gaagaccgug aaggcguccg acgucgccag ccuggugaug     1800

gccaagaccc gcggggugaa caagcccucc gucgagggcc ugagcgaccc gaagaaguuc     1860

aacuccaacc ucgccaccua ccuggaccug aagagcaucg ugggggacaa gguggacgac     1920

aaccgguacc agauggaccu cgagaacauc aucgaguggc gcuccgucuu cgaggacggc     1980

gagaucuucg ccgacaagcu gacggaggug gaguggcuga ccgacgagca gcggagcgcg     2040

cucgugaaga agcgcuacaa gggguggggc cgccugucca agaagcugcu caccggcauc     2100

gucgacgaga acgggcagcg gaucaucgac cugaugugga acaccgacca gaacuucaug     2160

cagaucguga accagcccgu guucaaggag cagaucgacc agcugaacca gaaggccauc     2220

acgaacgacg gcaugacccu ccgcgagcgg gucgagagcg ugcuggacga cgccuacacc     2280

uccccccaga acaagaaggc caucuggcag gugguccgcg ugguggagga caucgucaag     2340

gccgugggga acgcgcccaa gagcaucucc aucgaguucg cccggaacga gggcaacaag     2400

ggggagauca cccgcagccg gcgcacgcag cugcagaagc ucuucgagga ccaggcccac     2460

gagcugguga aggacaccuc ccugaccgag gagcucgaga aggucccgga ccugagcgac     2520

cgguacuacu ucuacuucac ccagggcggc aaggacaugu acaccgggga ccccaucaac     2580

uucgacgaga ucagcacgaa guacgacauc gaccacaucc ugccccaguc cuucgugaag     2640

gacgacagcc ucgacaaccg cgugcugacc ucccggaagg agaacaacaa gaagagcgac     2700

cagguccccg ccaagcugua cgcggccaag augaagccgu acuggaacca gcuccugaag     2760

cagggccuga ucacccagcg caaguucgag aaccucacca uggacgugga ccagacgauc     2820

aaguaccggu cccugggguu cgugaagcgc cagcuggucg agacccggca ggugaucaag     2880

cucaccgcca acauccuggg cagcauguac caggaggccg gcaccgacau caucgagacg     2940

cgcgccgggc ugaccaagca gcuccgggag gaguucgacc ugcccaaggu gcgcgagguc     3000

aacgacuacc accacgcggu ggacgccuac cugaccaccu ucgccggcca guaccucaac     3060

cggcgcuacc ccaagcugcg guccuucuuc guguacgggg aguacaugaa guucaagcac     3120

ggcagcgacc ugaagcuccg caacuucaac uucuuccacg agcugaugga gggggacaag     3180

ucccagggca aggucgugga ccagcagacg ggcgagcuga ucaccacccg cgacgaggug     3240

gccgacuacu ucgacugggu ccaggaccuc aagcugaugc uggugaccaa ggagcucaag     3300

cggagcaacg ggaagcuguu cgacgccacg auccagaacg cgaacuacac caagcugaac     3360

gagcgcaaga agguguucgu cccgcucaag aaggagcugg acaagacccu guacggcucc     3420

uacagcggga uccaguccag cuacaugacc aucgugaaga aguucgacaa gaagggcucc     3480

acguacgccg ugcagcggau ccccgucaug uacagcaagc uccuggagcu ggagaaggag     3540

caguccaagc ucaaccgccu ggugcagagc aucgugacca gcgaccccca ggcccccaac     3600

aacuucgagg uccugaugcc gugcgugcuc uccgggcagc uggucaucga caacggccgg     3660

aaguucaccc ugaccagcua ccgcuaccag ugcuccacgg cccagcucuu ccugcccaag     3720

aaggaccuga aguacaucca gaacaacuuc aaggagcuca agaagacccc cgacaagcag     3780

gacaagaucu ucgagaccau cuacgacgac auccugaagc agguggacaa guacuucagc     3840

cuguacgaca ucaacaaguu ccgguccaag cuccacgacg gcaaggacaa guuccuggcc     3900

cuggagaacu ucggggagaa ggagagcaag gacaagguga ucaccaagau ccuccgcggc     3960

cugcacgcga acgccgagcg gacgucccug aaggagaucg ggcucagcac cccccugggc     4020

caguuccagu ccggggaggg cggcaucacc cugagcgaca acaccaagau caucuaccag     4080

uccccgacgg ggcucuucga gcgccacguc uaccugaagg accuguga                  4128


<210>  2011
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2011
augcgcaaga accaggagcc cuacaacauc ggccuggaca ucgggaccag cuccaucggc       60

uggagcauca ugaacgacaa cuucgaccuc augcggguga aggggaagaa gggcaucggg      120

guccgccugu acaacgaggg ccaguccgcc gccgagcggc gcaugcaccg gaccgcgcgc      180

cggcgcuacg gccggcgcaa guggcggcug cgccuccugg aggacuucuu cgacgagcac      240

auggccgagg uggacgacac guucuucgcc cggcugaagg acagcaacau cuccccgaag      300

gacgacaaga aguaccgcaa gagccuccug uuccccaagu ccaagggggu gaccuaccag      360

gacgacggcg aguucuacaa gaaguacccc accauguacc accuccggua cgcccucaug      420

accgagcacc gcaaguucga ccugcgggag aucuaccugg ccuuccacca cauggucaag      480

uaccgcggga acuuccucua cgacacgagc guggacuccu ucgaggcgaa gaaccuggac      540

aucaagggca aguucgacga gaucaacgac cugcucagcu ccuacaccga cuucuacgug      600

gacaacagca acgccgcccu ggucgagucc auccugcucg agaagaacac cacccggaag      660

gacaagagca agaagaucgc caagcugcug cacguggagg acaaggagaa ggggaagaac      720

aagaaggcga aggaccucgc cacgcagauc uccaacgccg ugcugggccu gaagugcaac      780

uuccaccuca ucuucgagcu gcagcagaag uacagcuucg accugugcuc cgagaagacc      840

gaggagaaca ucgccaagcu cagcgagguc cuggacgaga accagaagau gcugcucaug      900

auccugaagg aggugcagga ccaggugaug cugaacgccu ucguccccuc cggcaugagc      960

cucagcgagg cgaugaugca gaaguacgac gacuacgggg agcagcugaa gaucuaccac     1020

gagcuggagc acuccgugag cgacgacucc gccgccaagc uccgcggcgc cuaccgggac     1080

uacaacgaca acaucaucaa gcgcaucgac ggggacaaca agaaggacac cuucuacaag     1140

cgcgugaaga acauccugac caagaucagc aaggaguacg ucgaccagga cgugcuggag     1200

uccugcggca agcucaagaa gcugaucgac gagaacaagc uguucauccg gcagcgcacg     1260

gccgcgaacg gcgugcuccc gcaccagcug caccagaucg agaugcggaa gaucaucgac     1320

aaccagaagg aguacuaccc cuggcuggcc gagcccaacc ccaacgagaa gcgccggguc     1380

uacagcaagu acaaggugga ggagcucauc gccuuccgca ucccguacua cguggggccc     1440

cuggucgacc ccaacaacgc cgacaagaac aaggaggccc gguucuccug gauggugcgc     1500

aagaaggacg gcgagaucac ccccuggaac uucuacgaca agguggacug ggcggagagc     1560

gccaacaacu ucaucgagcg gaugaagucc aaggacaccu accugcucgg ggaggacguc     1620

gugccgaagg agagcaugcu guaccagaag uacgaggucc ugaacgagcu gaacaaccuc     1680

cgcaucaacg acuccggccu gagcgacucc uucgaggacg ugaagcugaa gcaggccauc     1740

uacaacgacc ucuucaagaa gcagaagauc gugaagauca ccgaccugca gaacuaccug     1800

guccagaacc acaaguaccu cgugaagccc aagaucagcg ggcuggccga cgagaaccgg     1860

uuccugucca gccucuccac guacagcgac cugaagacca ucuucggcga caagguggac     1920

gaccgcaccu acuucaacga cuucgagaag auggucgagu acuccaccgu guucgaggac     1980

ggccacgacu acaaccagaa gcuggacgag uacacguggc ucaccaagga ggagaaguac     2040

aagaucggga agaagcggua ccgcggcugg gggaagcuga gcaagaagcu gcucaccggc     2100

cugcgggaca agaacaacca caccaucaug gacaaccugu gggagacgaa ccgcaacuuc     2160

augcagaucc agaccgccga cgaguucagc aagcagaucg cggaggagaa cgagcggcac     2220

cucaaggggu ccgugagcga cgccaucaac gacauguaca ccucccccgc caacaagaag     2280

gccauccgcc agguccugcg ggugguggac gacauccaga aggcgauggg cuacgccccc     2340

agcuccauca gccuggaguu cgcccgcgag gacggcccgu ccguccgcac cgugagccgg     2400

gccaaccgca ugaaguccau cuacgagaag uacgccagcg agguguccga ggaggucaug     2460

aaggaccucg acggggugau caaggacaag aagggccuga acgaccggcu guaccucuac     2520

uucgagcagc aggggaagga cauguacagc ggccaccccc uggacuucga caaggugauc     2580

uccgggcagg aguacgacau cgaccacauc cugccccagg cggucaucaa ggacgacagc     2640

cucgacaacc gcgugcugac gaccaaggcc cugaacaacg acgugaaguc caagggcguc     2700

cccugccgga uguucaacgg caugcacagc uucuggaaga accucuacga caagggguuc     2760

aucucccgcc ggaaguucaa caaccugacc accaacccgg agaacaucga caaguacaag     2820

augaagggcu ucgugaaccg ccagcuggug gagacgcggc agaucaucaa gcucgucgcc     2880

aacgugcuga acgacaagua ccagaacgac gacguggaca ucaucgaggu ccgcgccgag     2940

cugacccacg acgugcggaa gcacuucaag uucuacaaga accgcaacgu gaacgacuac     3000

caccacgccu ucgacgcgua ccucaccagc uucaucgggc acuaccuguu caagaaguac     3060

cccaaccugc ggccccucuu cgacuacaac gacuucauga aggucagcga caacguguuc     3120

aagcagcugc gcggcaacaa cuuccugggc gaguuccuca acaagaccgg ggacaucauc     3180

uccacggacg gcaacuucgu gcugaacaag gaggagauga ucaacaagcu gaacaaggcc     3240

uacgccuuca agaagauccu cgucaccaag gagguggggc agcggaccgg cgccauguuc     3300

aacgagaccc gcuaccccgc cccgaacagc cggaaggcgg cccugaagua ccgccggaug     3360

cccaagcccg acucccugau cagcgugaag gacuacaaga acaccgacau cuacgggggc     3420

uacuccggca agaacgacgc cuacaugguc aucguggaca uggggaagca guacauggug     3480

gucggcgugc ccgugcgcua cacggagaag cucgacaagc ugcggaucaa gcacagcgag     3540

cuguaccgcg acgagcuccg caagguccug ucccgggaca agacccugcu cgacagcaag     3600

gggaacguga agcgcuucga caucgugcug gaccgggucc uguacggcca gcucaucgag     3660

gacgggaacg agcuguucac ccugggcucc agcaaguaca agcgcaacuu ccggcagcug     3720

uuccuggaca agcagugcau cgagauccug gacuccagcg ccaccccgca gcccacggac     3780

gaggagcuca ucugggugua cgaccagauc cuggacaagg uggacaagua cuucgagcug     3840

uacgacauca accgccagcg ggagacccuc cgcaagggcc gggcggccuu cugcucccug     3900

cccaacauca gcaugaagaa ggacgacguc accaagaaga agauccugaa cgagauccuc     3960

guggcccugc acgccaacga guccgagagc aacugcgaga agaucgggaa gaagguguuc     4020

ggccgccugc agguccccgg gggcaucaag cucuccaagg aggccaagcu gaucuaccag     4080

agcccgaccg ggcuguucga gcgggugguc uaccucaagg accuguga                  4128


<210>  2012
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2012
auggagaaca agaaguacag caucggccug gacaucggga ccuccagcau cggcuucgcc       60

guggucgacg agaacaaccg cgugaugcgg gugaagggga agaacgccau cggcguccgc      120

cucuucuccg aggggcagcc cgcggccgac cggcgcagcu uccggaccac gcgccggcgc      180

cugucccggc gccgguggcg ccugcggcuc cugcgcgaga ucuucgagcc guacaucaac      240

cccguggacg aggccuucuu caugcggcug aaggagagca accucucccc caaggacgcc      300

aacaagcacu acagcggcga cauccuguuc aacaaccaga ccgaccagga cuucuacgag      360

aaguacccca ccaucuacca ccuccgccac gcccugauga ccgagcaccg gaaguucgac      420

gugcgcgaga ucuaccuggc gauccaccac aucgucaagu uccggggcca cuuccugaac      480

gccgccuccg ccagcaacuu cacggugggg aagcucaacc uggagggcaa guucgacgcg      540

cugaacgaga ucuaccagcg cgugcucacc gacgacccgu ucgcccugcg gaccgacaac      600

cuggaccaca ucaaggagau ccucaucgac aacaagcgcu cccaggccga ccgccagcgg      660

acccugacgg gggacgucua ccagagcucc gaggagaagg agaucgagaa gcgcaacaag      720

gccaucgcca ccgagauccu gaaggcggcc gugggcaaca aggccaagcu caacgugauc      780

accaacgucg agguggacaa ggaggccgcc aaggacugga gccugaccuu cgacuccgag      840

acgaucgacg acgagcuggc gaagguggac gggcagauga ccgacgacgg ccgggagauc      900

aucgagaucc uccgcagccu guacuccggc aucacccugg ccgccaucgu ccccgagaac      960

cacacccuca gccaguccau gguggagaag uacaacagcc acaaggccca ccugaagcug     1020

cucaaggacc ugaucaacaa caugacggac accaagaagg ccaagaaccu gcgggcggcc     1080

uacgacgggu acaucgacgg cgugaagggg aagguccuca cccgcgacga cuucuacaag     1140

cagauccagg gcaaccugga cgacagcgcc gaggcccaga agauccagga cgugauggac     1200

caggacgagu ucaugcccaa gcagcggacc aaggccaacg gcuccauccc ccaccagcug     1260

cagcagcagg agcucgacca gaucguggag aaccagaagg aguacuaccc guggcuggcg     1320

gaggagaacc ccaacgagac gcgccgcggg gucgccaagu acaagcugga cgagcucgug     1380

accuuccggg ugcccuacua cgucggcccc augaucaccg ccgaggacca ggagaagcag     1440

agcggggccu ccuucgcgug gaugaagcgg aaggagccgg gcaacaucac ccccuggaac     1500

uucgaccaga agguggaccg cauggccagc gccaaccagu ucaucaagcg gaugacgacc     1560

accgacaccu accugcuggg ggaggacguc cuccccgccc agucccugcu guaccagcgc     1620

uucgaggugc ucaacgagcu gaacaagauc cggaucgacc gcaagcccau cagcaucgag     1680

cugaagcagc agaucuucaa cgaccucuuc aagaaguuca agaacgugac gaucaagcac     1740

cuccaggagu accuggucgc caccggcggc uacgacaagc ggccgcgcau cgacgggcug     1800

gcggacgaga agcgguucaa cuccggccug agcgccuacc acgaccugca cgggauccug     1860

ggcgccgagg ugguggacgc caacgacaag caggacgacc ucgagaagau caucgagugg     1920

uccaccaucu ucgaggacaa gaagaucuac cgcgccaagc uggacgagcu gaccuggcuc     1980

acggacagcc agaaggagaa gcuggcgacc aagcgcuacc aggggugggg ccggcugucc     2040

aagaagcucc ugagcggccu gcgcaacucc gaccaccgga gcauccucga cauccugugg     2100

aucaccaacg agaacuucau gcagauccag gccgaccccg acuucgccga gcuggucaag     2160

aacgccaacc aggggaugcu cggcaagaac aacucccagg acgugaucaa cgaccuguac     2220

accagccccc agaacaagaa ggccauccgc cagauccugc ucguggucca cgacauccag     2280

aacgcgaugc acgggcaggc ccccgccaag auccacgugg aguucgcccg gggcgaggag     2340

cgcaacccgc ggcgcuccau ccagcggcag caccagaucg aggcggccua cgacaaggug     2400

acgagcgacc ugcuggucga cgccggggug cgccaggagc ucaaggacgc caucaacgac     2460

aagcgggacu ucaaggaccg ccuguuccug uacuucaugc agggcggccg ggacaucuac     2520

aacggggagc ccaucaacau cgaccgccuc uccagcuacg acaucgacca cauccugccc     2580

caggccuucg ugaaggacga cagccugaac aaccgggucc ucaccgacgc ggagggcaac     2640

cgccggaagu ccgacgccgu gcccagcgag cuguucgggg ccaagaugcg cuccaccugg     2700

gagcugaugc gggagcaggg ccucaucagc aaggccaagu accgcaaccu gaccaugaac     2760

ccggagaaca ucaacgccca caccgagaac ggcuucauca accggcagcu gguggagacg     2820

cgccagguca ucaagcucgc ggugaacauc cuggccgacg aguacaccga ggacacccag     2880

aucguguccg ucaaggccga ccugagccac cagaugcgcg aggacuucga gcuccugaag     2940

aaccgggacg ugaacgacua ccaccacgcc uucgacgccu accuggcggc cuucaucggg     3000

aacuaccucc uggagcgcua ccccaagcug caguccuacu ucguguacgg cgacuucaag     3060

aaguucagcc agaaggacac caagaugcgg cgcuucaacu ucaucuacga ccucaagcac     3120

uccgaccaga ucgccgacaa gaacgggaag aagaucuggc acaagacgaa cgaccuggac     3180

uacauccggc accuguucgc cuacaagaag auccucauca gccacgaggu cuccgagcgc     3240

cggggcgcgc uguacaacca gaccguguac aaggccagcg acgacaaggc cuccgggcag     3300

ggcagcaaga agcugauccc caucaaggcc aacaagccca ccgagaucua cggcggguac     3360

accggcaaga cgcucgccua cauggcgauc gugaagguca ucaaggggaa gaagaucgcc     3420

uacaaggugg ugggcguccc gaccuccgcc cuggugcagc uggccgggau cgagcacggc     3480

agccaggagg agcucaaccg ccuguacgac gugcugaagc cccaguucac ccagcucaag     3540

aagaacaaca agaccggcga gaucuccgag acggucagca aguucgagau cgugaucccc     3600

cgggugaagu accagcagcu ggucgaggac gacgacaagc uguucaugau cgggagcgac     3660

accuacccgu acaacgccca gcagcucgug cuguccgagg gcgcgcugaa ggugaucaac     3720

aacgucuacc gcaaggggga gaacagcgac aagcaggagu ccuacaagcu cgacgacgug     3780

uacgacgaga ucaucaucgc caugaagaag uacuuccccc uguacgacaa ccggggcuuc     3840

cgcgagaagg uggccgaggg caagagcacc uucgagaacc ugcccaucuc caacgaguuc     3900

aagaacggga agcucaccaa gaucggcaag aaggagguca ucaaccggau gcugcagggg     3960

cugcacgcca acgccacgau gaccgaccuc aagauccugg gcaucaagag ccccuucggg     4020

uucaugcagc agccgaccgg caucgcgcug uccgagaacg cccagcucau cuaccagagc     4080

cccaccgacc uguucgagcg ccgggugcag cugaacaaga ucaaguga                  4128


<210>  2013
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2013
augaagaaga uccugggccu cgaccugggg accaccagca ucggcugggc ccugguguac       60

gaguccgaga acacggacga gaaguccagc aucguccgcc ucggggugcg ggugaacccc      120

cugagcgucg acgagaaggg caacuucgag aaggggaagg ccaucaccac caacgcggac      180

cgccagcaga cccacggcgc ccggaucaac cugcagcgcu acaagcuccg gcgcgagaac      240

cugaaggacu gccugcagaa gcagggcugg aucgggacgg aggcccucug cgaggagggc      300

aagaccucca ccuucgagac cuacaugcug cgggccaagg ccgcggagga ggagaucagc      360

cugcacgagc ucugccgcgu gcuguucaug augaacaaga agcgggggua caaguccaac      420

cgcaaggcca acaacaagga ggacggcaag cuguucgacg ggaugacggu ggccaagaag      480

cucuacgagg agcaccugac cccggccgag uacagcuucc agcugcucuc cagcggcaag      540

aaguucaagc aggaguacua ccgguccgac cugaaggagg aguucgaccg caucuggaac      600

gagcagcaga aguacuaccc cgagauccug accgacgacu ucaagcagca gcuccagggc      660

aagagcaagg ggaacaccuc caagaucuuc cugggcaagu acgggaucua cagcgcggac      720

cugaagggcc ucgaccggaa gcugcagccc cugaaguggc gcgucgaggc ccucaagcag      780

caggucgaca aggaggugcu ggccuucgug aucuccgacc ugaacggcca gaucgccagc      840

acguccgggc uccugggcgc caucagcgac cgguccaagg agcuguacuu caacaagcag      900

accguggggc aguaccucug gaccagccug aaggagaccc cgcacgugag caucaagaac      960

aagccguucu accgccagga cuaccuggac gaguucgaga agaucuggga gacgcaggcg     1020

accuaccaca aggagcucac ccccgagcug aagcaggaga uccgggacau caucaucuuc     1080

uaccagcgcc cccugaaguc caagaagggc cucaucagcg ucugcgagcu ggagcagcgg     1140

aaggugaagg ugaccaucga cgggaaggag aaggagaucu ccaucggccc caaggucgcc     1200

cccaagagcu cccccauguu ccaggaguuc cgcaucuggc agaaccugaa caacauccuc     1260

cugaucgaca agggcaacaa cagcauccac ccccuguacg aggaggagaa gaaccuccug     1320

uacgaggagc uguccgugaa ggagaagcuc agcaaggccg aggcccugaa ggugcugaac     1380

aagaacucca agcaguggga ccugaacuac aaggagaucg aggggaaccg gacgcaggcc     1440

guccucuacg acugcuacaa ccgcaucauc gugaugaccg gccacgagga guacaacuuc     1500

aagaagcuga aggcgagcga gauccgcucc uacgugaaga ccaccuucac ggggcugggc     1560

uucaacaccg acauccucga guucgacagc ucccugaccg ggcacgagcu ggagaagcag     1620

cccauguacc agcucuggca ccuccuguac agcuacgagu ccgacaacag ccggaccggc     1680

aacgacgccc ugcugcgcaa gcuggagacg gccuucggcu ucccgaagga guacgccgcc     1740

guccucuccg acgugguguu cgaggacagc uacgggaacc uguccgucaa ggcgaugcgg     1800

aagauccugc cccaccucca ggccggccug aacuacagcc aggccugcga ggaggccggg     1860

uacaagcaca gccgccgguc ccugaccaag aacgagcucg accagaagga guacaaggag     1920

aagcuggagc ugcuccccaa gaacagccug cacaaccccg uggucgagaa gauccugaac     1980

cagaugauca acgugaucaa cgccgugauc gacgaguacg gcaagccgga cgagauccgc     2040

aucgagcucg cgcgggagcu gaagaucggg gccgccgagc gcgagaacau gacccggucc     2100

aucaaccagg gcaacgccga gaacaagcgc auccgggaga uccuggagaa ggaguucgcg     2160

cucagcuaca ucucccgcaa cgacaucauc aaguacaagc uguacgagga gcucaagagc     2220

aacggcuuca agacccugua cuccgacacg uacaucagca aggacaagcu guucuccaag     2280

gacuucgaca ucgagcacau cauccccaag gcccggcugu ucgacgacag ccucuccaac     2340

aagaccaucg aggcccgcga cgucaacauc gccaagagca acaagaccgc cuucgacuuc     2400

aucuccgaga aguacgacca gcaggggauc gaggcguaca agaagaagcu ggacauccug     2460

cuccaggacg gcagcaucuc caaggccaag uacaaccggc ugcugaugaa ggaggccgac     2520

auccccagcg gguucaucga ccgcgaccuc cgggacaccc aguacaucgc caagaaggcc     2580

uacgagaucc uguccgaccu ggugcgcaac gugacgccca ccaccggcgc ggucaccagc     2640

cggcuccgcg aggacuggca gcugguggac gugaugaagg agcugaacuu cgagaaguac     2700

cagaagcucg gccuggucga ggugaucgag gaccgcgacg ggcggaagau caagcgcauc     2760

aaggacugga ccaagcggaa cgaccaccgc caccacgcca uggacgcccu gacgaucgcc     2820

uucaccaagc cgagcuacau ccaguaccuc aacaaccuga acgcccgguc cgacaagaac     2880

agcuccaucu acggcaucga gaacaaggag cugcaccgcg aggacaacaa gcuccgguuc     2940

aacgcgcccc ugcccaucaa cgaguuccgc gccgaggccc ggcgccaccu caagagcauc     3000

cugguguccg ucaaggccaa gaacaaggug augacccaga acgugaacgu caucaagacc     3060

aagcacgggg ugaucaagaa ggugcagcug acgccccggg gcgagcugca caacgcgacc     3120

uucuacggga ccaagaagcg cccgaucauc aagaugguca aggugggcgc cgcccucgac     3180

gaggugacca ucaagaaggu cagcuccagc uucauccggg aggcccugcu ggaccgccuc     3240

cacgaguaca acggcgacgc caagaaggcg uucacggggc ggaacuccau cgacaagaac     3300

cccaucuaca ucgacgagga gcacaagaag accgugcccg cccuggugaa gaccaucgag     3360

ugggagaugu accaccccac ccgcaagcug aucagcccgg accucaagau cgacaagguc     3420

aucgacaagg gcauccggga gauccugaag acgcgccugg ccaaguucaa cggggacgcc     3480

cagaaggccu ucuccaaccu cgacgagaac cccaucuaca aggacgaggc gaagaagauc     3540

acccugaagc gggugagccu ggagggcgug uccagcguca ucccccucca cacccugaag     3600

gaccgcgagg ggaagcuggu ggugggcaag gacggcaagc cgguccucgg ggacuacgug     3660

cagaccucca acaaccacca caucgccuuc uacauggacg agaacggcaa ccugcaggac     3720

aacgugguca gcuucuucga ggccgccgag cggaaguccc agggggugcc cgugaucgac     3780

aagaacuaca accacgacaa gggcuggaac uuccuguuca cgaugaagca gaacgaguac     3840

uucgucuucc ccaacgaggu gaccggguuc aaccccagcg agguggaccu cauggacgag     3900

gccaacuacu ccaucaucag cccgaaccug uaccgcgucc agaagaugag caaggcgucc     3960

ggccgcgacu acugguuccg gcaccaccug gaggccaugc ucaacgacga ccccaagcug     4020

cgcaacagca uguucaagcg gaucaccucc cugaaggccc ucgagggcau cgugaaggug     4080

cgcaucaacu gcaucgggaa gaucgucgcc gugggcgagu acgacuga                  4128


<210>  2014
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2014
augagcaaca aguccuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucaccg acgacuacaa ggugcccagc aagaagauga aggugcucgg gaacacggac      120

aagcacuuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcg      180

gaggaccgcc ggcugaagcg caccgcccgg cgccgguaca cgcgccggaa gaaccgccug      240

cgguaccucc aggagaucuu cucccaggag aucagcaagg ucgacuccag cuucuuccac      300

cgccuggacg acuucuuccu ggugccggag gacaagcggg gcuccaagua ccccaucuuc      360

gccacccucg uggaggagaa ggaguaccac aagaaguucc ccaccaucua ccaccuccgc      420

aagcaccugg ccgacagcaa ggagaagacc gaccuccggc ugaucuaccu ggcccucgcg      480

cacaugauca aguaccgcgg ccacuuccug uacgaggagu ccuucgacau caagaacaac      540

gacauccaga agaucuucag cgaguucauc uccaucuacg acaacacguu cgagggcaag      600

agccuguccg ggcagaacgc ccaggucgag gccaucuuca ccgacaagau cagcaagucc      660

accaagcggg agcgcgugcu caagcuguuc cccgacgaga aguccaccgg gcuguucagc      720

gaguuccuca agcugaucgu gggcaaccag gccgacuuca agaagcacuu cgaccuggag      780

gagaaggcgc cgcuccaguu cagcaaggac acguacgacg aggaccugga gaaccugcuc      840

gggcagaucg gcgacgacuu cgccgaccug uuccuggucg ccaagaagcu cuacgacgcc      900

auccugcuga gcggcauccu caccgugaag gaccugucca ccaaggcgcc ccugagcgcc      960

uccaugaucg agcgguacga gaaccaccag aaggaccucg ccgcccugaa gcaguucauc     1020

cagaacaacc ugcaggagaa guacgacgag guguucagcg accaguccaa ggacggguac     1080

gccggcuaca ucgacgggaa gaccacgcag gaggccuucu acaaguacau caagaaccuc     1140

cugagcaagu ucgagggcgc ggacuacuuc cuggacaaga ucgagcgcga ggacuuccuc     1200

aagaagcagc ggaccuucga caacggcucc aucccccacc agauccaccu ccaggagaug     1260

aacgccauca uccgccggca gggggagcac uaccccuucc ugcaggagaa caaggagaag     1320

aucgagaaga uccugaccuu ccgcaucccg uacuacgucg gcccccuggc ccgcgggaac     1380

ggcgacuucg ccuggcugac ccggaacagc gaccaggcca uccgccccug gaacuucgag     1440

gagaucgugg accaggcguc cagcgccgag gacuucauca acaagaugac gaacuacgac     1500

cucuaccugc ccgaggagaa ggugcugccg aagcacuccc ugcucuacga gaccuucgcc     1560

gucuacaacg agcugaccaa ggugaaguuc aucgccgagg ggcugacccg guaccaguuc     1620

cucgacaaga agcagaagaa ggacaucuuc gacacguucu ucaaggccga gaacaagcgc     1680

aaggugaccg agaaggacau cauccacuac cugcacaacg ucgacggcua cgacggcauc     1740

gagcugaagg ggaucgagaa gcaguucaac gcgagccucu ccaccuacca cgaccugcug     1800

aagaucauca aggacaaggc cuucauggac gacagcaaga acgaggagau ccucgagaac     1860

aucauccaca cccugacgau cuucgaggac cgggagauga ucaagcagcg ccuggcccag     1920

uacgacuccc ucuucgacga gaaggugauc aaggcccuga cccggcgcca cuacaccggc     1980

ugggggaagc ugagcgcgaa gcucaucaac ggcauccggg acaagaaguc cgggaagacc     2040

auccuggacu accugaucga cgacggcgag aucaaccgca acuucaugca gcucauccac     2100

gacgacggcc ugagcuucaa ggagaucauc cagaaggccc agguguucgg gaagacgaac     2160

gacgucaagc aggucgugca ggagcugccc ggcucccccg ccaucaagaa ggggauccuc     2220

cagagcauca agaucgugga cgagcuggug aaggucaugg gccacgcccc cgagagcauc     2280

gugaucgaga uggcccggga gaaccagacc accgcgcgcg ggaagaagaa cucccagcag     2340

cgguacaagc gcaucgagga cagccugaag aaccucgccu ccggccugga cagcaacauc     2400

cugaaggaga acccgaccga caacauccag cuccagaacg accggcuguu ccuguacuac     2460

cuccagaacg gccgcgacau guacacgggg aagccccugg agaucaacca gcuguccaac     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagccu cgacaaccgg     2580

gugcugaccu ccagcaagga caaccgcggc aaguccgaca acguccccag ccuggaggug     2640

guggagaaga ugaaggccuu cuggcagcag cuccuggacu ccaagcugau cagcgagcgg     2700

aaguucaaca accucaccaa ggccgagcgc gagcgcgggg gccugaacga gcuggacaag     2760

gucggcuuca ucaagcggca gcucguggag acccgccaga ucaccaagca cguggcgcag     2820

uuccuggacg cccgguucaa caaggagguc acggagaagg acaagaagaa ccgcaacgug     2880

aagaucauca cccugaaguc caaccucgug agcaacuucc ggaaggaguu cgggcuguac     2940

aagguccgcg agaucaacga cuaccaccac gcccacgacg ccuaccugaa cgccgugcuc     3000

gcgaaggcca uccugaagaa guacccgaag cuggagcccg aguucgugua cggcgacuac     3060

cagaaguacg accuccagcg guacaucucc aagagccgcg agcccaagga ggucgagaag     3120

gccacccaga aguacuucuu cuacuccaac cugcugaacu ucuucaagga ggaggugcac     3180

uacgccgacg ggaccaucgu gaagcgggag aacaucgagu acagcaagga cacgggcgag     3240

aucgcgugga acaaggagaa ggacuucgcc accgucaaga aggugcucag ccugccccag     3300

gugaacaucg ucaagaagac cgaggugcag accgggggcu ucuccaagga gagcauccug     3360

ccgaagggca acuccgacaa gcucaucccc cgcaagacga aggagauccu gugggacacc     3420

accaaguacg ggggcuucga cagccccgug aucgccuacu ccauccugcu caucgccgac     3480

aucgagaagg ggaaggccaa gaagcugaag accgucaaga cgcugguggg caucaccauc     3540

auggagaagg cgaccuucga gaagaacccc aucaccuucc ucgagaacaa gggguaccac     3600

aacgugcgga aggagaacau ccugugccug ccgaaguaca gccucuucga gcuggagucc     3660

ggccgccggc gcaugcuggc cagcgccaag gagcuccaga agggcaacga gaucguccug     3720

cccguguacc ugacgacccu ccuguaccac uccaagaacg ugcacaagcu ggacgagccc     3780

gagcaccucg aguacaucca gaagcaccgg uacgaguuca aggaccugcu gaaccucguc     3840

agcgaguucu cccagaagua cgugcuggcc gacgccaacc uggagaagau caagaaccuc     3900

uacgcggaca acgagcaggc cgacaucgag auccuggcca acagcuucau caaccugcuc     3960

accuucaccg cccugggggc cccggcggcc uucaaguucc ugggcaagga cguggaccgc     4020

aagcgguaca cgaccgucuc cgagauccuc aacgccaccc ugauccacca gagcaucacc     4080

gggcuguacg agacgcgcau cgaccucucc aagcugggcg aggacuga                  4128


<210>  2015
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2015
auggagaaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccagcaag aaguucaagg ugcucgggaa cacgaaccgc      120

aaguccauca agaagaaccu gaugggcgcc cugcuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgcauccgg      240

uaccugcagg agaucuucgc caacgagaug gccaagcucg acgacuccuu cuuccagcgc      300

cuggaggaga gcuuccuggu cgaggaggac aagaagaacg agcggcaccc gaucuucggc      360

aaccucgcgg acgagguggc cuaccaccgc aacuacccca ccaucuacca ccuccggaag      420

aagcuggccg acucccccga gaaggccgac cuccgccuga ucuaccuggc gcucgcccac      480

aucaucaagu uccggggcca cuuccugauc gaggggaagc ugaacgccga gaacagcgac      540

guggccaagc uguucuacca gcugauccag accuacaacc agcuguucga ggagagcccc      600

cucgacgaga ucgaggucga cgccaagggc auccuguccg cgcgccuguc caagagcaag      660

cggcucgaga agcugaucgc cguguucccg aacgagaaga agaacgggcu guucggcaac      720

aucaucgccc ucgcccuggg gcugaccccc aacuucaagu ccaacuucga ccucacggag      780

gacgccaagc ugcagcugag caaggacacc uacgacgacg accucgacga gcugcugggc      840

cagaucggcg accaguacgc ggaccucuuc uccgccgcca agaaccugag cgacgccauc      900

cugcucuccg acauccugcg cagcaacucc gaggugacca aggcgccccu gagcgccagc      960

auggucaagc gguacgacga gcaccaccag gaccucgccc ugcugaagac ccucgugcgc     1020

cagcaguucc ccgagaagua cgccgagauc uucaaggacg acacgaagaa cggguacgcc     1080

ggcuacgugg ggaucggcau caagcaccgg aagcgcacca ccaagcuggc cacccaggag     1140

gaguucuaca aguucaucaa gccgauccug gagaagaugg acggcgcgga ggagcuccug     1200

gccaagcuga accgcgacga ccugcuccgg aagcagcgca cguucgacaa cggguccauc     1260

ccccaccaga uccaccugaa ggagcugcac gccauccucc ggcgccagga ggaguucuac     1320

cccuuccuga aggagaaccg ggagaagauc gagaagaucc ugaccuuccg cauccccuac     1380

uacgucggcc cgcucgcccg ggggaacagc cgcuucgcgu ggcugacccg gaaguccgag     1440

gaggccauca cccccuggaa cuucgaggag gugguggaca agggcgccag cgcccagucc     1500

uucaucgagc gcaugacgaa cuucgacgag cagcugccca acaagaaggu ccuccccaag     1560

cacagccugc uguacgagua cuucaccgug uacaacgagc ucaccaaggu gaaguacguc     1620

accgagcgga ugcgcaagcc ggaguuccug uccggggagc agaagaaggc caucguggac     1680

cuccucuuca agacgaaccg gaaggugacc gucaagcagc ugaaggagga cuacuucaag     1740

aagaucgagu gcuucgacag cguggagauc aucggcgugg aggaccgcuu caacgcgucc     1800

cugggcaccu accacgaccu ccugaagauc aucaaggaca aggacuuccu ggacaacgag     1860

gagaacgagg acauccucga ggacaucguc cugacccuga cgcuguucga ggaccgggag     1920

augaucgagg agcgccugaa gaccuacgcc caccuguucg acgacaaggu gaugaagcag     1980

cucaagcggc gccacuacac cggguggggc cggcugagcc gcaagaugau caacgggauc     2040

cgcgacaagc aguccggcaa gaccauccug gacuuccuca agagcgacgg guucuccaac     2100

cggaacuuca ugcagcugau ccacgacgac agccugacgu ucaaggagga gaucgagaag     2160

gcccaggucu ccggccaggg cgacagccuc cacgagcaga ucgccgaccu ggccgggucc     2220

cccgcgauca agaagggcau ccugcagacc gugaagaucg uggacgagcu cgucaaggug     2280

auggggcaca agcccgagaa caucgugauc gagauggccc gcgagaacca gaccaccacg     2340

aagggccugc agcagagccg ggagcgcaag aagcggaucg aggaggggau caaggagcug     2400

gagucccaga uccucaagga gaaccccguc gagaacaccc agcugcagaa cgagaagcug     2460

uaccucuacu accugcagaa cggccgcgac auguacgugg accaggagcu ggacaucaac     2520

cggcucagcg acuacgacgu ggaccacauc gucccgcaga gcuucaucaa ggacgacucc     2580

aucgacaaca aggugcugac ccgcagcgug gagaaccggg gcaaguccga caacgucccc     2640

agcgaggagg uggugaagaa gaugaagaac uacuggcgcc agcugcucaa cgccaagcug     2700

aucacccagc ggaaguucga caaccugacc aaggccgagc gcgggggccu cuccgaggcc     2760

gacaaggcgg gguucaucaa gcggcagcug gucgagacgc gccagaucac caagcacgug     2820

gcccggaucc uggacagccg caugaacacc aagcgggaca agaacgacaa gcccauccgc     2880

gaggugaagg ucaucacccu caaguccaag cuggugagcg acuuccggaa ggacuuccag     2940

cuguacaagg ugcgcgacau caacaacuac caccacgccc acgacgccua ccucaacgcg     3000

gucgugggca cggcccugau caagaaguac cccaagcugg aguccgaguu cguguacggc     3060

gacuacaagg ucuacgacgu gcggaagaug aucgccaaga gcgagcagga gaucgggaag     3120

gccaccgcca agcgcuucuu cuacuccaac aucaugaacu ucuucaagac cgaggugaag     3180

cucgcgaacg gcgagauccg caagcggccg cugaucgaga ccaacgggga gacgggcgag     3240

gucgugugga acaaggagaa ggacuucgcc accgugcgca agguccuggc caugccccag     3300

gugaacaucg ugaagaagac cgagguccag accgggggcu ucagcaagga guccauccuc     3360

agcaagcggg aguccgccaa gcugaucccc cgcaagaagg gcugggacac gcggaaguac     3420

gggggcuucg ggagccccac cguggccuac uccauccugg uggucgcgaa gguggagaag     3480

ggcaaggcca agaagcucaa gagcgugaag guccuggugg ggaucaccau cauggagaag     3540

ggcagcuacg agaaggaccc gaucggcuuc cuggaggcca agggguacaa ggacaucaag     3600

aaggagcuca ucuucaagcu gcccaaguac ucccuguucg agcucgagaa cggccgccgg     3660

cgcaugcugg ccagcgccac cgagcugcag aaggcgaacg agcucgugcu gccccagcac     3720

cugguccggc uccuguacua cacgcagaac aucuccgcca ccaccgggag caacaaccug     3780

ggcuacaucg agcagcaccg cgaggaguuc aaggagaucu ucgagaagau caucgacuuc     3840

uccgagaagu acauccucaa gaacaaggug aacagcaacc ugaaguccag cuucgacgag     3900

caguucgccg uguccgacag cauccugcuc uccaacagcu ucgucucccu gcugaaguac     3960

accagcuucg gggccuccgg cggcuucacg uuccucgacc uggacgugaa gcaggggcgg     4020

cugcgcuacc agaccgugac cgagguccuc gacgcgaccc ugaucuacca gagcaucacg     4080

ggccuguacg agacccggac cgaccucucc cagcucgggg gcgacuga                  4128


<210>  2016
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2016
augaagaaga uccugggccu cgaccugggg accaccagca ucggcugggc ccuggugaac       60

gaguccgaga acaaggacga gcagagcucc aucguccgcc ucggggugcg ggugaucccc      120

cugacggucg acgagaaggg caacuucgag aaggggaagg ccaucaccac caacgcggac      180

cgccagcagg uccacgccgc ccggaucaac cugcagcgcu acaagcuccg gcgcgagaac      240

cugcgggacu gccugcagaa gcagggcugg aucggcagcg agccgaugug cgaggacggg      300

aaguccagca ccuucgagac guacaagcuc cgcgccaagg ccgcggugga ggaggugucc      360

cugcacgagu ucgcccgggu gcuguucaug cucaacaaga agcgcggcua caagagcaac      420

cggaaggcca acgacaagga ggaggggaag cuguucgacg gcaugaccau cgccaagaag      480

cuguacgagg agcaccucac ccccgcggag uacucccugc agcugcucaa caccgggcgc      540

aaguucacgc ccgaguacua ccggagcgac cugaucgagg aguucgagaa gaucuggaac      600

gagcagcaga aguacuaccc cgagauccug accgacgagu ucaagcagca gcucgagggc      660

aaggccaagu ccaacaccag caagcuguuc cugggcaagu acgggaucua cuccgccgac      720

accaagggcc ucgaccgccg gcugcagccg cugaaguggc gcguggaggc caugaagcag      780

caggucgaca aggacgugcu cgccuucgug aucagcgacc ugaaggggca gaucgcgaac      840

acguccggcc ugcucggcgc caucagcgac cgguccaagg agcuguacuu cagcaagcag      900

accaucgggc aguaccugug ggccucccuc caggagaacc cccacaucag caucaagaac      960

cagcccuucu accgccagga cuaccuggac gaguucgaga agaucuggga gacccaggcc     1020

accuaccaca aggagcugac gagcgagcuc aagcaggaga uccgggacau caucaucuuc     1080

uaccagcgcc cccugaaguc caagaagaac cugaucgccg ucugcgagcu cgagcagcgg     1140

aaggugaagg ugaccgucga cggcaaggag aaggagauca ccaucgggcc gaagaucgcg     1200

cccaagagcu cccccauguu ccaggaguuc cgcaucuggc agaaccugaa caacauccug     1260

cuccuggacc acgagaccaa cagcaagcgc ccccuguacg aggaggagcg gaaccuccug     1320

uacgaggagc uguccgugaa ggagaagcuc agcaagacgg aggcccugaa ggugcugggc     1380

aagaagucca agcaguggga ccugaacuac aaggagcucg aggggaaccg cacccaggcc     1440

guccuguacg acugcuacaa ccggaucauc gugaugagcg gccacgagga gugcaacuuc     1500

aagaagcuga aggccuccga cauccgccac uacgugaaga ugaucuucaa gaaccucgcc     1560

uucaacaccg acauccugug cuucgacagc ucccugaagg gccacgagcu cgagcggcag     1620

ccgaugugcc agcuguggca ccuccuguac agcuacgagg gggacaacuc ccgcaccggc     1680

aacgacgcgc ugcugcggaa gcucgagacg agcuucgggu uccccaagga guacgccgcc     1740

guccuggccg acgugaucuu cgaggacucc uacggcaacc ugagcgugaa ggccaugcgc     1800

aacauccucc cccaccucca ggcggggaag gacuacuccc aggccugcga ggaggccggc     1860

uacaagcaca gccggcgcuc ccugaccaag gaggagcuga accagaaggu cuacaaggag     1920

aagcuggagc ugcuccccaa gaacagccug cacaacccgg uggucgagaa gauccugaac     1980

cagaugguga acgugaucaa cgccgucauc gaggaguacg gcaagcccga cgagauccgg     2040

aucgagcucg cgcgcgaccu gaagaucggg gccgccgagc gggagaacau gacccgcagc     2100

aucaaccagg gcaacgccga gaacaagcgg auccgcgaga uccuggagaa ggaguucgcc     2160

cucuccuacc ugagccggaa cgacaucauc aaguacaagc uguacgagga gcucaagucc     2220

aacggguuca agacccugua cagcgacacg uacaucucca aggacaagcu guucagcaag     2280

gacuucgaca ucgagcacau cauccccaag gcgcgccucu ucgacgacuc ccugagcaac     2340

aagaccaucg aggcccggga cgugaacauc gccaagucca acaagaccgc cuucgacuuc     2400

auccuggagc gcuacggcga gcagggggug aagaccuaca agacgaagcu ccugcgccug     2460

cucgaggacg gcgccaucag caacaagaag uacaacaacc ugcugaagac cgaggcggac     2520

auccccuccg gcuucaucga ccgggaccuc cgcaacaccc aguacaucgc caagaaggcc     2580

uacgagaucc ugagcgagcu ggucaagauc gugaccccga cgaccggguc cgugaccaac     2640

cggcuccgcg aggacuggca gcuggucgac gugaugaagg agcugaacuu cgagaaguac     2700

cagaagcucg gccuggccga cgugaucgag gaccgggacg ggcgcaaggu caagcggauc     2760

aaggacugga ccaagcgcaa cgaccaccgg caccacgcca uggacgcgcu gaccaucgcc     2820

uucacgaagc ccagcuacau ccaguaccuc aacaaccuga acgcccgcuc cgacaagagc     2880

uccagcaucu acggcaucga ggugaaggag cugcaccggg aggagggcaa guuccgcuuc     2940

aacgcgcccc uccccaucaa cgaguuccgg gccgaggcca agcgccaccu caagagcgug     3000

cuggucucca ucaaggccaa gaagaaggug augacccaga acgugaacaa gaucaagacc     3060

aagcacggga ucaucaagaa gguccagcug accccgcggg gcgagcugca caacgccacg     3120

uacuacggga ccaagaagcg cccccuggug cggaugguga aggucggcgc cgcgcucgac     3180

gaggccacca ucaagcgcgu gugcagcccc gccauccggg ccgcccugcu gaagcgccuc     3240

aacgcguaca acggggacgc caaggaggcc uucaccggcc ggaacuccau cgacaagaac     3300

cccaucuacc uggacaucga gcagacgaag cuggugccgg uccucgugaa gaccaucgag     3360

uggggcaugu accaccccau ccgcaagcgc aucacccccg accugaagau cgacaaggug     3420

aucgacaagg ggauccggga gaagcugaag gcccgccucg ccgaguucaa cggcgacgcg     3480

cagaaggccu ucagcaaccu ggacgagaac ccgaucuaca uggacgaggc caagcggauc     3540

acccugaagc gcgucuccau cgagggggug cucagcgcca ucccccugca cacgcugaag     3600

gacaaggcgg gcaagcccgu gaucgggucc gacggcaagc ccgucuucgg cgacuacgug     3660

cagaccagca acaaccacca caucgccuuc uacaucgacg acaaggggaa ccuccaggac     3720

aacccggugu ccuucuucga ggccgccgag cgcaagagcc agggcguccc cgugaucgac     3780

cggcgguaca acugcgacaa gggguggcac uucguguuca ccaugaagca gaacgaguac     3840

uucgucuucc ccaacgaggc caccggcuuc auccccuccg agguggaccu gcuggacgag     3900

gcgaacuacu gcaucaucag cccgaaccuc uaccgcgugc agaagcuguc caaggccagc     3960

gggcgggacu acugguuccg ccaccaccug gagacgaugc ucaacgacga ccugcggcug     4020

aagaacucca ccuucaagcg cauccagagc cucaagggcc uggaggacau cgucaaggug     4080

cggaucaacu ccaucggcaa gaucguggcc gucgggaagu acgacuga                  4128


<210>  2017
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2017
auggagacca aguucaccua cuaccugggc cucgacaugg ggacggacag ccugggcugg       60

gccgugaccg acuccagcua ccacauccug cgcgccaagg ggaaggaccu cuggggcguc      120

cggcuguucc ccgcggccga gaccuccgug gaccgccgga ccuaccgcgu gaaccggcgc      180

cggcgccagc gggaggucgc ccgcaucggg gcccugaagg agcuguucgc cgacgagauc      240

gagaaggugg acccgggcuu cuucgugcgg cuggacgaga gcaaguucca cauggaggac      300

cgcuccgaga acaacaagca gaaguacggc cuguucgcgg acaagcuguu cagcgacgcc      360

gaguacuaca agaaguaccc cacgaucuuc caccuccgga aggagcuccu cgaguccgag      420

gagccccacg acguccgccu gguguaccug gcccuccuga acauguucaa gcaccggggg      480

cacuuccuga acagcucccu ccagaucgag ggcgagaaca gcaacaucgc cgaguccuac      540

cagauguucg uggacacccu gaaggacgag gagcuggacu uccccgagca ggucagcggg      600

gagcgccucc aggagaucuu ccgguccagc gacucccgca agaagaaggu ggagaaccug      660

cuggcggaga ugaaccucag caagaagcag aagcgggaga uccaguucgu gaaccugauc      720

ugcggccuga aggggaagcu cuccgagcug uucccggaca aggaguacga cgaggagcag      780

aagaagaucg ccuucagcuu ccaggacucc gacuacgagg acaaggagaa caccauccag      840

gagcugcucg acgagagcga gaugaacaug aucuacgccg ucaaggccgu gcacgacagc      900

ggcgugcugu ccggcaucau gaacggggag cgcuaccuga gcgucgcccg gguggcgucc      960

uacgagaagc acaagaagga ccuccagauc cugaaggaca uccugaagaa guacgacacc     1020

aaggaguacu ucgagauguu ccgcaacgag agcgagaagu ccgagaacua cagcgccuac     1080

gugggcuccg ucaaguacca cgggacguuc cgccggggcc ucagcggcgg gaagcaggag     1140

gaccuguaca agcacaucaa gaaggugcug ggcaaguucc cccaggacga cgagggggug     1200

accuacgucc ucgaggagau cgagaccgag uccuuccugc ccaagcagcu gaccgccagc     1260

aacggcguga uccccaacca ggugcacgcc cgcgagaugc ucgccauccu gaagcacgcg     1320

gagcuguacc ucccguuccu gcaggagaag gacgacacgg ggcuguccgu cagcgagaag     1380

auccucaagc uguucauguu ccagaucccc uacuacgugg gcccccuggg ccaggaguac     1440

aaggacaaga agggguacaa cgucugggcc gagcggaagg agcccggcaa gaucuacccg     1500

uggaacuucg aggagaagau cgacgugaag aaguccgccg agaaguucau cgggcgcaug     1560

gugcggcgcu gcaccuaccu gagcggcgag caggcccucc ccaagcaguc ccugcuguac     1620

gagaaguucc agguccucaa cgagcugaac aaccugcgga uccgcgggga gaagaucccc     1680

gugagccuca agcaggagau cuaccacgac cuguucggcg acggcaagaa gguguccaug     1740

acccggcuga ccaacuaccu ccugcagaac ggggucauca gcuccgagga gaaggacagc     1800

ugcaucagcg gcaucgacgg gggcuuccac aacucccuga gcuccguggg gaaguucaag     1860

ggcguguucg gcgacacggu ccacaccgac gagcaccgca agaugaucga ggacaucauc     1920

uucuggggga ccguguucgg cgacgggaag aagcuccuga aggagaaggu ggaggagguc     1980

uacggcaacc agcugaccga ggcccagaag aagcggaucu ucggcuucaa guucagcggg     2040

uggggccgcc ucuccaaggc guuccugacg auggaggggg ccagcaagga caacggcgag     2100

aucaaggggc ugauccaggc caugugggac accaacugca accucaugga gcugcugucc     2160

gagcgguuca ccuacaagga caagcucaac gagaugguga ccacggccga gaagccccug     2220

ugcgagugga agaucgagga ccuggacgag auguaccuca gcgccccggu gaagcgcaug     2280

gucuggcaga ccauccgggu gguggacgag guccagcaga ucaugggcug cccgcccaag     2340

cgcaucuucg uggagaugac ccgguccgac ggcgagaagg ggaagcgcac cgugagccgg     2400

cagaagaagc uggucgaccu guacaaggcg cucggcaagg aggggaagau cugguccgag     2460

gagcugaaca acacgcccga gggccaguuc cgcaucaaga agcuguaccu cuacuaccug     2520

cagaugggga agugcaugua caccggcgag agcaucgagc uggagccccu caugaacgac     2580

aaccaccugu acgacaucga ccacaucuac ccccgccacu ucaucaagga cgacaacauc     2640

uccaacaacc uggugcucgu gaagaaggag cggaacgccc acaagagcga cuccuucccc     2700

aucgagcccg agauccgcaa caagaugaug ccguucugga agucccugcg gaagaagggc     2760

uuccugaccg aggagaagua caaccgccuc acccggauga gcgccuucac ggacgaggag     2820

aaggccgcgu ucaucagccg ccagcugguc gagaccagcc aggggaccaa ggccaucacc     2880

cagaucuuca agcaggccuu cgccgacucc gagaucgugu ucagcaaggc cuccgugguc     2940

agcgcguucc ggcagaaguu cgacugcuac aaggugcgcu gcgugaacga cuaccaccac     3000

gcccacgacg ccuaccugaa caucgucgcc ggcaacacgu accgggugaa guucaccucc     3060

aacccccuca acuucaucaa ggaggccaag aagaacccca acagccgcga gaaccgguac     3120

aacauggaca agaccuucga gugggacgug acccgcaacg gggagaccgc gugggucgcc     3180

caguccaacg agaaccccgg cacgauccag cuggugaaga aguaccugaa caagaacagc     3240

ccgcucauca ccaagauggu guacgagaag cacgggggca ucacccagaa ggccaccauc     3300

ugguccgcca agaaggccga gagcggcaac acggacgcgu acauccccgu caagaugaac     3360

gacccccggc uggccgacgu gaccaaguac gggggcguga ccaccaucgc cgucgccggg     3420

uacacgcugc ucgaguacaa ggcgggcggg aagaccgugc gcucccugga ggcccugccg     3480

aucuaccucg gcggcagcaa gaaccugacc gaggagcaga ugguggagua cuucuccaac     3540

auccugcagc aggagaacaa ggagaagguc gugagcgacg ugaucauccg gaagaagaug     3600

auccccuccg ggagccucau caaguacaac ggcuucuacu acuaccuggc cgggaagacc     3660

ggcaaccgca ucguccugau cuccgccgug cagcucugcc ugcccaugga cacgaugcug     3720

uacaucaaga agaucgagaa ggccugcagc accggcaacu ucgaggagaa ggacaagaac     3780

gggaagcagg uccucuccaa gcagcagaac gaguucgugu acgagcugau caucgagaag     3840

uaccagcaga gcaucuucaa gaagaaggug ggcgcgaucg gggacaccau caucgagaag     3900

cgggagaagu ucgccgagau cagccuggag gagcagugca aggugcucau gcagaucuuc     3960

cugaacuucc aguccggcau cggggucgac cugaccagcg uggacggcuc caacaagagc     4020

ggcgucacgu accucaacaa gaagaucaug aacgcccagg agcugaagcu gaucgaccag     4080

uccgugaccg ggguguucag ccgcgagauc gaccuccuga ccaucuga                  4128


<210>  2018
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2018
augagcaaca aguccuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucaccg acgacuacaa ggugcccagc aagaagauga aggugcucgg gaacacggac      120

aagcacuuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcg      180

gaggaccgcc ggcugaagcg caccgcccgg cgccgguaca cgcgccggaa gaaccgccug      240

cgguaccucc aggagaucuu cuccgaggag augagcaagg ucgacuccag cuucuuccac      300

cgccuggacg acuccuuccu ggugccggag gacaagcggg gcagcaagua ccccaucuuc      360

gccacccucg ccgaggagaa ggaguaccac aagaaguucc ccaccaucua ccaccuccgc      420

aagcaccugg ccgacuccaa ggagaagacc gaccuccggc ugaucuaccu ggcgcucgcc      480

cacaugauca aguaccgcgg ccacuuccug uacgaggaga gcuucgacau caagaacaac      540

gacauccaga agaucuucuc cgaguucauc agcaucuacg acaacacguu cgaggagucc      600

agccugaucg ggcagaacgc ccagguggag gccaucuuca ccgacaagau cuccaagagc      660

gcgaagcggg agcgcguccu caagcuguuc cccgaccaga aguccaccgg ccuguucagc      720

gaguuccuca agcugauccu ggggaaccag gccgacuuca agaagcacuu cgaccucgag      780

gagaaggccc cgcugcaguu cagcaaggac accuacgacg aggaccugga gaaccuccug      840

ggccagaucc gggacgacuu cgccgaccug uuccucgugg ccaagaagcu guacgacgcg      900

auccugcucu ccgggauccu gacggugaag gaccugagca ccaaggcgcc ccucuccgcc      960

agcaugaucg agcacuacga gaaccaccag aaggaccugg ccgcccugaa gcaguucauc     1020

cagaacaacc uccaggagaa guacgacgag gucuucuccg accagagcaa ggacggcuac     1080

gccggcuaca ucaacgggaa gaccacccag gaggccuucu acaaguacau caagaaccug     1140

cuguccaagu ucgagggcgc cgacuacuuc cucgacaaga ucgagcgcga ggacuuccug     1200

cggaagcagc gcacguucga caacgggagc aucccccacc agauccaccu ccaggagaug     1260

aacgccaucc ugcaccggca gggcgagcac uaccccuucc ugaaggagaa caaggagaag     1320

aucgagaaga uccugaucuu ccgcaucccg uacuacgugg gcccccucgc ccgcgggaac     1380

cgggacuucg cguggcugac ccgcaacucc gacgaggcca uccggcccug gaacuucgag     1440

gagaucgugg acaaggccag cuccgccgag gacuucauca acaagaugac caacuacgac     1500

cuguaccugc ccgaggagaa gguccucccg aagcacagcc ugcuguacga gaccuucgcc     1560

guguacaacg agcucacgaa ggugaaguuc aucgcggagg gccugacccg cuaccaguuc     1620

cuggacaaga agcagaagaa ggacaucuuc uacaccuucu ucaaggccga ggacaagcgg     1680

aaggucaccg agaaggacau cauccacuac cuccacacgg uggacgggua cgacggcauc     1740

gagcuggagg ggaucgagaa gcaguucaac gccucccuga gcaccuacca cgaccuccug     1800

aagaucauca aggacaagga guucauggac gacgacaaga acgaggccau ccuggagaac     1860

aucgugcaca cccucaccau cuucgaggac cgcgagauga ucaagcagcg gcuggcgcag     1920

uacaacuccc uguucgacga gaaggucauc aaggcccuca cgcgccggca cuacaccggc     1980

uggggcaagc ugagcgccaa gcugaucaac gggauccgcg acaagaaguc cggcaagacc     2040

auccucgacu accugaucga cgacggggag aucaaccgga acuucaugca gcugauccac     2100

gacgacggcc ucagcuucaa ggagaucauc cagaaggccc agguguucgg gaagaccaac     2160

gacgugaagc aggucgugca cgagcugccc ggcucccccg ccaucaagaa gggcauccug     2220

cagagcauca agaucgugga cgagcucguc aaggugaugg ggcacgcgcc cgagagcauc     2280

gucaucgaga uggcccgcga gaaccagacg accgcccggg gcaagaagaa cucccagcag     2340

cgcuacaagc ggaucgagga cagccugaag aaccuggccu ccgggcucga cagcaacauc     2400

cugaaggaga acccgaccga caacgugcag cugcagaacg accgccucuu ccuguacuac     2460

cugcagaacg gccgggacau guacaccggg aagccccucg agaucaacca gcuguccaac     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagccu ggacaaccgc     2580

gugcucacgu ccagcaagga caaccggggc aaguccgaca acguccccag ccuggaggug     2640

guggagaaga ugaaggcguu cuggcagcag cugcucgacu ccaagcugau cagcgagcgc     2700

aaguucaaca accugaccaa ggccgagcgc gagcggggcg ggcucaacga gcuggacaag     2760

gucggcuuca ucaagcgcca gcugguggag acccggcaga ucaccaagca cguggcccag     2820

auccucgaca cccgcuucaa caaggagguc acggagaagg acaagaagaa ccggaacgug     2880

aagaucauca cccugaaguc caaccuggug agcaacuucc gcaaggaguu cgggcucuac     2940

aagguccggg agaucaacga cuaccaccac gcccacgacg ccuaccugaa cgcgguggug     3000

gccaaggcca uccugaagaa guacccgaag cucgagcccg aguucgucua cggcgaguac     3060

cagaaguacg accugaagcg cuacaucucc cggagcaagg accccaagga caucgagaag     3120

gccaccgaga aguacuucuu cuacuccaac cugcucaacu ucuucaagga ggaggugcag     3180

uacgcggacg gcaccaucgc caagcgcgag aacaucgagu acagcaagga cacgggggag     3240

aucgccugga acaaggagaa ggacuucgcc accgugaaga agguccugag ccugccccag     3300

gugaacaucg ugaagaagcg ggagguccag accggcgggu ucuccaagga gagcauccuc     3360

ccgaagggca acuccgacaa gcugaucccc cgcaagacca aggacauccu gugggagacg     3420

accaaguacg ggggcuucga cagccccgug aucgccuacu ccauccuccu gaucgcggac     3480

aucgagaagg gcaagagcaa ggagcugaag accgugaaga cccucgucgg gaucacgauc     3540

auggagaagg ccaccuucga ggagaacccc aucaccuucc uggagaacaa gggcuaccac     3600

aacgugcgga aggagaacau ccugugccuc ccgaaguacu cccuguucga gcuggagaac     3660

gggcgccggc gccuccuggc cagcgccaag gagcugcaga agggcaacga gaucgugcuc     3720

cccgucuacc ugaccacgcu gcucuaccac uccaagaacc ugcacaagcu ggacgagccc     3780

gggcaccucg aguacaucca gaagcaccgg aacgaguuca aggaccugcu gaaccucgug     3840

agcgaguucu cccagaagua cauccuggcc gacgcgaacc uggagaagau ccagaaccuc     3900

uacgccgaca acgagcaggc cgacaucgag auccuggcca acagcuucau caaccugcuc     3960

accuucacca cccugggcgc cccggcggcc uucaaguucu ucggcaagga caucgaccgc     4020

aagcgguaca cgaccguguc cgagauccug aacgccaccc ucauccacca gagcaucacc     4080

gggcuguacg agacgcgcau cgaccugucc aagcucggcg aggacuga                  4128


<210>  2019
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2019
auggagaagu ccuacagcau cggccuggac aucgggacca acagcguggg cugggccguc       60

aucaccgacg acuacaaggu gcccgccaag aagaugaagg ugcucgggaa cacggacaag      120

aaguacauca agaagaaccu gcugggcgcg cuccuguucg acuccgggga gaccgccgag      180

gucacccgcc ugaagcggac cgcccgccgg cgcuacacgc ggcgcaagaa ccggcuccgc      240

uaccugcagg agaucuucgc caaggagaug accaaggugg acgagagcuu cuuccagcgg      300

cuggaggagu ccuuccucac cgacgacgac aagaccuucg acagccaccc gaucuucggc      360

aacaaggccg aggaggacgc guaccaccag aaguucccca cgaucuacca ccuccgcaag      420

uaccuggccg acucccagga gaaggccgac cuccggcugg uguaccuggc ccucgcgcac      480

augaucaagu accgcggcca cuuccugauc gagggggagc ugaacgccga gaacaccgac      540

guccagaagc uguucaacgu guucguggag accuacgaca agaucgucga cgagagccac      600

cuguccgaga ucgaggugga cgccagcucc auccugaccg agaaggugag caagucccgg      660

cgccucgaga accugaucaa gcaguacccc acggagaaga agaacacccu guucggcaac      720

cucaucgccc uggcccuggg gcuccagccc aacuucaaga ccaacuucaa gcugagcgag      780

gacgcgaagc ugcaguucuc caaggacacc uacgaggagg accucgagga gcugcugggc      840

aaggucgggg acgacuacgc cgaccucuuc aucagcgcca agaaccugua cgacgccauc      900

cugcucuccg gcauccugac gguggacgac aacagcacca aggccccgcu guccgcgagc      960

augaucaagc gguacgugga gcaccacgag gaccucgaga agcugaagga guucaucaag     1020

aucaacaagc ugaagcucua ccacgacauc uucaaggaca agaccaagaa cggcuacgcc     1080

ggguacaucg acaacggcgu caagcaggac gaguucuaca aguaccugaa gaccauccug     1140

acgaagaucg acgacagcga cuacuuccuc gacaagaucg agcgcgacga cuuccugcgg     1200

aagcagcgca ccuucgacaa cggguccauc ccccaccaga uccaccucca ggagaugcac     1260

agcauccugc ggcgccaggg cgacuacuac cccuuccuga aggagaacca ggccaagauc     1320

gagaagaucc ugaccuuccg gauccccuac uacgugggcc cgcuggcccg caaggacucc     1380

cgcuucgccu gggcgaacua ccacagcgac gagcccauca cccccuggaa cuucgacgag     1440

guggucgaca aggagaaguc cgccgagaag uucaucacgc ggaugacccu caacgaccug     1500

uaccugcccg aggagaaggu gcucccgaag cacagccacg uguacgagac cuucaccguc     1560

uacaacgagc ugacgaagau caaguacgug aacgagcagg gggaguccuu cuucuucgac     1620

gccaacauga agcaggagau cuucgaccac guguucaagg agaaccgcaa ggucaccaag     1680

gccaagcugc ucagcuaccu gaacaacgag uucgaggagu uccggaucaa cgaccugauc     1740

ggccucgaca aggacuccaa gagcuucaac gccucccugg ggaccuacca cgaccugaag     1800

aagauccucg acaagagcuu ccuggacgac aagaccaacg gccagaucau cgaggacauc     1860

gugcugacgc ucacccuguu cgaggaccgc gacaugaucc acgagcggcu gcagaaguac     1920

uccgacuucu ucaccagcca gcagcucaag aagcuggagc gccggcacua caccgggugg     1980

ggccgccugu ccuacaagcu caucaacggc auccggaaca aggagaacaa caagacgauc     2040

cuggacuucc ugaucgacga cgggcacgcg aaccgcaacu ucaugcagcu caucaacgac     2100

gagagccugu ccuucaagac caucauccag gaggcccagg ucgugggcga cguggacgac     2160

aucgaggccg ucgugcacga ccugcccggg agccccgcca ucaagaaggg cauccuccag     2220

uccgugaaga ucgucgacga gcuggugaag gugauggggg acaaccccga caacaucguc     2280

aucgagaugg cgcgggagaa ccagaccacc ggcuacggcc gcaacaagag caaccagcgg     2340

cugaagcgcc uccaggacag ccugaaggag uucggguccg acauccugag caagaagaag     2400

ccguccuacg uggacagcaa gguggagaac ucccaccucc agaacgaccg gcuguuccug     2460

uacuacaucc agaacggcaa ggacauguac accggggagg agcucgacau cgaccgccug     2520

agcgacuacg acaucgacca caucaucccc caggccuuca ucaaggacaa cuccaucgac     2580

aacaaggucc ugacgagcuc cgccaagaac cggggcaaga gcgacgacgu gcccuccauc     2640

gagaucgugc gcaaccggcg cagcuacugg uacaagcucu acaaguccgg gcugaucagc     2700

aagcgcaagu ucgacaaccu gaccaaggcc gagcggggcg gccucaccga ggccgacaag     2760

gcgggguuca ucaagcgcca gcuggucgag acccggcaga ucacgaagca cguggcccag     2820

auccuggacg cccgcuucaa caccaagcgg gacgagaacg acaaggugau ccgcgacguc     2880

aaggugauca cccucaaguc caaccuggug agccaguucc ggaaggaguu caaguucuac     2940

aagguccgcg agaucaacga cuaccaccac gcccacgacg ccuaccugaa cgcgguggug     3000

ggcaccgccc uccugaagaa guaccccaag cugacgccgg aguucgucua cggggaguac     3060

aagaaguacg acgugcggaa gcucaucgcc aaguccagcg acgacuacag cgagaugggc     3120

aaggccaccg ccaaguacuu cuucuacucc aaccugauga acuucuucaa gaccgaggug     3180

aaguacgcgg acggccgcgu cuucgagcgg cccgacaucg agaccaacgc cgacggggag     3240

guggugugga acaagcagaa ggacuucgac aucguccgca aggugcugag cuacccccag     3300

gugaacaucg ucaagaaggu ggaggcccag acgggcgggu ucuccaagga gagcauccuc     3360

uccaagggcg acagcgacaa gcugaucccc cggaagacca agaaggugua cuggaacacc     3420

aagaaguacg ggggcuucga cuccccgacc gucgccuaca gcgugcuggu ggucgcggac     3480

aucgagaagg gcaaggccaa gaagcucaag acggugaagg agcugguggg gaucuccauc     3540

auggagcgca gcuucuucga ggagaacccc gucuccuucc uggagaagaa gggcuaccac     3600

aacgugcagg aggacaagcu caucaagcug cccaaguaca gccuguucga guucgagggc     3660

gggcggcgcc ggcuccuggc cuccgccacc gagcugcaga aggggaacga ggugaugcuc     3720

cccgcccacc uggucgagcu gcucuaccac gcgcaccgca ucgacagcuu caacuccacc     3780

gagcaccuga aguacgugag cgagcacaag aaggaguucg agaaggugcu guccugcguc     3840

gagaacuuca gcaaccucua cguggacgug gagaagaacc uguccaaggu ccgggccgcc     3900

gccgagagca ugaccaacuu cagccuggag gagaucuccg ccagcuucau caaccuccug     3960

acgcugaccg cgcucggcgc cccggccgac uucaacuucc ugggcgagaa gaucccccgc     4020

aagcgguaca ccuccaccaa ggagugccug agcgccacgc ucauccacca guccgugacc     4080

gggcuguacg agacccgcau cgaccugagc aagcucggcg aggaguga                  4128


<210>  2020
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2020
augaccaaga ucaaggacga cuacaucgug ggccuggaca ucgggaccga cagcugcggc       60

ugggucgcca ugaacuccaa caacgacauc cucaagcugc aggggaagac ggccaucggc      120

agccgccugu ucgagggcgg gaaguccgcg gccgagcggc gccucuuccg gaccacccac      180

cgccggauca agcgccggcg cuggcggcug aagcugcucg aggaguucuu cgaccccuac      240

auggccgagg uggacccgua cuucuucgcc cgccugaagg agagcggccu gagcccccuc      300

gacaagcgga agaccgugag cuccaucguc uuccccacga gcgccgagga caagaaguuc      360

uacgacgacu accccaccau cuaccaccuc cgcuacaagc ugaugaccga ggacgagaag      420

uucgaccucc gggaggugua ccuggcgauc caccacauca ucaaguaccg cgggaacuuc      480

cuguacaaca ccuccgugaa ggacuucaag gccagcaaga ucgacgucaa guccagcauc      540

gagaagcuca acgagcugua cgagaaccug ggccucgacc ugaacgugga guucaacauc      600

uccaacacgg ccgagaucga gaaggugcug aaggacaagc agaucuucaa gcgggacaag      660

gucaagaaga ucgccgagcu guucgcgauc aagaccgaca acaaggagca gagcaagcgc      720

aucaaggaca ucuccaagca ggucgccaac gccgugcugg gguacaagac ccgguucgac      780

accaucgccc ugaaggagau cagcaaggac gagcucuccg acuggaacuu caagcugagc      840

gacaucgacg ccgacuccaa guucgaggcg cugaugggca accucgacga gaacgagcag      900

gccauccugc ugacgaucaa ggagcuguuc aacgagguga cccucaacgg gaucguggag      960

gacggcaaca cccugagcga gagcaugauc aacaaguaca acgaccaccg cgacgaccuc     1020

aagcugcuga aggaggugau cgagaaccac aucgaccgga agaaggccaa ggagcucgcc     1080

cuggccuacg accuguacgu caacaaccgc cacggccagc uccugcaggc gaagaagaag     1140

cuggggaaga ucaagccgcg guccaaggag gacuucuaca agguggugaa caagaaccuc     1200

gacgacagcc gcgccuccaa ggagaucaag aagaagaucg agcuggacag cuucaugccc     1260

aagcagcgca ccaacgccaa cggcgucauc cccuaccagc ugcagcagcu cgagcuggac     1320

aagaucaucg agaaccaguc caaguacuac cccuuccuga aggagaucaa cccggugagc     1380

ucccaccuca aggaggcccc cuacaagcug gacgagcuga uccgguuccg cgugcccuac     1440

uacgucgggc cccugaucag cccgaacgag uccacgaagg acauccagac caagaagaac     1500

cagaacuucg ccuggaugau ccggaaggag gagggccgca ucacccccug gaacuucgac     1560

cagaaggugg accggaucga gagcgcgaac aaguucauca agcgcaugac cacgaaggac     1620

accuaccucu ucggcgagga cgugcugccc gccaacuccc ugcucuacca gaaguucacc     1680

guccugaacg agcugaacaa cauccggauc aacgggaagc gcaucagcgu ggaccucaag     1740

caggagaucu acgagaaccu guucaagaag cacaccacgg ucaccgugaa gaagcuggag     1800

aacuaccuca aggagaacca caaccuggug aaggucgaga ucaagggccu ggccgacgag     1860

aagaaguuca acuccgggcu caccaccuac aaccgguuca agaaccugaa caucuucgac     1920

aaccagaucg acgaccugaa guaccgcaac gacuucgaga agaucaucga guggagcacg     1980

aucuucgagg acaaguccau cuacaaggag aagcuccgga gcaucgacug gcugaacgag     2040

aagcagauca acgcccuguc caacauccgc cuccagggcu gggggcggcu gagcaagaag     2100

cugcucgccc agcugcacga ccacaacggc cagaccauca ucgagcagcu gugggacucc     2160

cagaacaacu ucaugcagau cgugacccag gcggacuuca aggacgccau cgccaaggcc     2220

aaccagaacc uccugguggc gaccagcguc gaggacaucc ugaacaacgc cuacacgagc     2280

cccgccaaca agaaggccau ccgccaggug aucaaggugg ucgacgacau cgugaaggcc     2340

gcguccggca aggugccgaa gcagaucgcc aucgaguuca cccgggacgc cgacgagaac     2400

cccaagcgca gccagacccg gggguccaag cuccagaagg ucuacaagga ccugagcacc     2460

gagcuggccu ccaagacgau cgccgaggag cucaacgagg cgaucaagga caagaagcug     2520

gugcaggaca aguacuaccu guacuucaug cagcucggcc gcgacgccua caccggggag     2580

cccaucaaca ucgacgagau ccagaaguac gacaucgacc acauccugcc ccagagcuuc     2640

aucaaggacg acgcccugga caaccgggug cucgucuccc gcgccgugaa caacggcaag     2700

agcgacaacg ugccggucaa gcuguucggg aacgagaugg ccgcgaaccu gggcaugacc     2760

auccgcaaga ugugggagga guggaagaac aucggccuca ucuccaagac caaguacaac     2820

aaccugcuga ccgaccccga ccacaucaac aaguacaaga gcgccggguu cauccggcgc     2880

cagcucgugg agacguccca gaucaucaag cuggugagca ccauccugca gucccgguac     2940

cccaacaccg agaucaucac cgucaaggcc aaguacaacc acuaccuccg cgagaaguuc     3000

gaccuguaca agagccggga ggugaacgac uaccaccacg ccaucgacgc guaccugucc     3060

gccaucugcg gcaaccuccu guaccagaac uaccccaacc ugcgcccguu cuucguguac     3120

gggcaguaca agaaguucag cuccgacccc gacaaggaga aggccaucuu caacaagacg     3180

cggaaguuca gcuucaucag ccagcuccug aagaacaagu ccgagaacag caaggagauc     3240

gccaagaagc ugaagcgcgc cuaccaguuc aaguacaugc ucgucucccg ggagaccgag     3300

acccgcgacc aggagauguu caagaugacc guguaccccc gguucagcca cgacacggug     3360

aaggcgcccc gcaaccugau cccgaagaag augggcaugu cccccgacau cuacgggggc     3420

uacaccaaca acagcgacgc cuacaugguc aucgugcgga ucgacaagaa gaagggcacc     3480

gaguacaaga uccuggggau ccccacccgc gagcucguga accugaagaa ggccgagaag     3540

gaggaccacu acaaguccua ccugaaggag auccucacgc cgcggauccu guacaacaag     3600

aacggcaagc gcgacaagaa gaucaccagc uucgagaucg ucaaguccaa gauccccuac     3660

aagcagguca uccaggacgg ggacaagaag uucaugcugg gcagcuccac cuacguguac     3720

aacgccaagc agcucacccu gagcacggag uccaugaagg ccaucaccaa caacuucgac     3780

aaggacagcg acgagaacga cgcgcugauc aaggccuacg acgagauccu cgacaaggug     3840

gacaaguacc ugccccuguu cgacaucaac aaguuccggg agaagcucca cuccggccgc     3900

gagaaguuca ucaagcugag ccuggaggac aagaaggaca ccauccucaa ggugcuggag     3960

gggcugcacg acaacgccgu gaugaccaag auccccacga ucggccucuc caccccgcug     4020

ggguucaugc aguuccccaa cggcgucauc cugagcgaga acgccaagcu caucuaccag     4080

ucccccaccg ggcuguucaa gaaguccgug aagaucagcg accuguga                  4128


<210>  2021
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2021
augagcaacu ucgacaacca caaggacucc aucugcaagc acaagggccu gaagaagggg       60

aacuacuaca ucggccucga caucgggacc aacagcgugg gcugggccgc caccaacgag      120

caguacgagc ugcugaaguu caagucccac aagauguggg ggagccgccu cuucgcggag      180

gcccagacgg ccgucgacac ccggaugaag cgcuccgccc ggcgccggcu gcagcgccgg      240

aaguuccgcc ugauccuccu ggagagccug uucuccaagg agaucaacgc cguggacgag      300

accuucuuca ugcggcucca cgagagcaac uaccacgugg aggacaagac caaccugguc      360

aaguacacgc uguucaucga caagaacuac accgacgugg acuacuacaa ggaguucccc      420

accaucuacc accuccgcca ccaccugaug accgagggca cgaaggacau ccggcugcug      480

uuccuggcga uccaccacau ccugaaguac cgcggcaacu uccucuacgg gaagaaggag      540

uucuccgugg acggcgagau guacagcguc cugcggucca cccuggagga gcucggguuc      600

ggcagcuacg uggaggacga gaacgugcug caguccaucg ccaaccuggu ccucgaccgc      660

gggaucgccc ggagcgagaa ggugaagcag aucgagccgc ugaucgccca gggcaagaag      720

cuguccagcg uggagaagaa ccgccucaag gcgugggccu accugaucau gggccugaag      780

gccaaccucg ucaaccuguu caccgacgag gacguggagg ugguccugcc cgaggagaag      840

gaguucaagu ccaucaaccu ccacgagggg aucuacgagg acgugcggga cgccuacgag      900

gacgccuggg gcgaccgcuu cgcgaucgug gaccagugca agguccugca cgacaucauc      960

gugaucagcu ccaucaucaa gcccgggcag agccugagcg aggccaagau cgaguccuac     1020

aaccagcaca aggaggaccu cgugcugcuc aagagcgucc ucaaggagga caagaagcug     1080

uacgacgaca uguucguggu ggacaucgac aagggcacca acuacgucaa guacaucaag     1140

cagggcguga acgggaacac guccaccacc caggaggccu ucuacaagua cguccggaag     1200

guggugagcg uccugcccga gaccgagaac acgcgcugca ucgugcagaa gcucgaggag     1260

ggcaccuucc ugccgcugca gcggaucucc aagaacgggg ugguccccua ccagcuccac     1320

aaggaggagc uggugcgcau ccuggagaag gccaagcuca acuuccccuu ccugaacgag     1380

guggaggacg gccugaccgu cgccgacaag aucaucagca uccuggaguu caagaucccc     1440

uacuacgugg ggccgcucaa ccccacccac uccaucgaga acggcggcuu cgcgugggug     1500

guccgcaagg aggccggcca ggugcugccc uggaacuucg agcagaaggu ggacguccag     1560

gccagcgccg ugaaguucau cgagaaccug acgaacaagu gcaccuaccu caucgaccag     1620

gacgugcugc ccaaguacuc ccugcucuac gccgaguuca gccugcugaa cgagcucaac     1680

aacauccggg ucaacgacaa gccgcugccc gcggacguga agcaggccuu cauccgcgac     1740

cacuucaucu cccugcagga cagcaagacc gccaccaaga agguggucgc caaguaccuc     1800

guggacaaca acuacguguc ccagccgccc aucaucacgg ggauggacca gcagaucgcc     1860

accaagcuga ccagccaccg ggacaugacc cgcauccugg gggagcacuu cgacuacgcg     1920

auggccgagg acaucauccg guacaucacg aucuucggcg aguccaagga caugcuccgc     1980

cagguccuga agaacuacua cggggaccgg cugaccaccg agcagaucaa gaagcucggc     2040

aagcugaccu acacgggcug ggggcgccug agcaagaagu uccucuccga caucuggggc     2100

augaagaaga acgacgacga cgccgaggcc gggaccauca ucgagcacau gcgggagggc     2160

caggagaacc ugaugcagcu gcucagcucc agcuacagcu ucaucgacgu ggugcaggag     2220

uacgucacca acgugcuggg ggcgaaggag aagaccgccu ucgagaugcu ggaggaccuc     2280

ucccugagcc ccauggugaa gcgcuccguc uggcagacgc ugcggauccu cgacgagauc     2340

gugagcaucc gcaaggagcu gcccaagaag aucuucgugg aggucgcccg gaccaacaag     2400

gccgacaaga agcgcaccca gucccggaag gcccugcucg aggagcugua cgagcagauc     2460

aaggacgagg acgugcacgu gcugcagcag gagcucgaga gcaaggagga cuccgcgcug     2520

aacagcaaga agcuguaccu cuacuucacc cagaugggcc gcugcaugua cuccggccgg     2580

cgcaucgaca uccacgagcu guucaccgac acguacgaca ucgaccacau cuacccccgg     2640

agccugacca aggacgacuc cuggcacaac aucguccucg uggagaagca gcugaacgcc     2700

gagaagaccg accgcuuccc cauccugagc gccauccagu ccaagcagaa gggguucugg     2760

aagcuccugu gggaccgcgg cuucaucagc gagaagaagu acgaccggcu ggugcgcacc     2820

acggaccuca ccgacgagga gcugaaccag uucaucaacc ggcagaucgu cuccaccaac     2880

cagagcguga aggccgugac cacgcugcuc caccagcugu acccggagac cgaggucgug     2940

uucuccaagg ccgagaacau cagcgacuuc cgccgggacu acggguucgu gaagguccgc     3000

agcaucaacc accaccacca cgcgaaggac gccuaccuga acaucguggu gggcaacguc     3060

uaccacgaga aguucaccaa gaacuuccuc uccuucgcca agcagaaggg cacccaccgg     3120

acguacaacc ugaccaagau guucgaguac gagaucaagc ugaccgagga gagcaagaag     3180

gugaucuggg accccaacau ccacauggac aucgugaaga agaugaugaa guccaacgac     3240

guccgcguga cccagcgggc cgucccccag aagggcgggc ucagcgacga gacgaucuac     3300

aagaagggga ucgccaagcg cggcguguac cugccccuga aguccagcga cgagcgggug     3360

cgcgacgucg agaaguacgg gggccggacc uccaucacca ucagcgagua caucauccuc     3420

aacauccgcg acaccuccga ccaggaccgg aucgagaucu ucagccugcc gcuguacauc     3480

gcgggcaaga aggacaucca caaggccauc gaggaggccu accucuccac gugcaagaag     3540

cccgccagcg ugguggacau ccagauccug uaccgcgggc uguacaucgg cuccaagguc     3600

aacaucaacg gguucaccua cuacaugggc gggaagaccg gcagcaacgu guccguggac     3660

agcgcggucg acguggugau ggacaucacc uccagcgucu acaucaagcu ccuggagaag     3720

guggccauca agaagaucga gaacuccaag acguuccgga ucgagaacau caagaccagc     3780

uacaaccaca aggaggugac cgucagcaag gagcagaaca ucgcccugua ccaggagcuc     3840

cugaagaagc uggacacgcc ccucuucucc aagaugaagg gcaacaagcu ggaggaguuc     3900

cgcaccgugg gguucgacac cuucaucgcc cugagccucg aggaccaggc cuccgugcug     3960

cuggaggucc ucaacgugcu gaccaacuuc aagaccaagc gggacaucac gaagaucggc     4020

augaccagcu cccgcagcac ccuggggauc aacaucucca acaccgagca guucagcgug     4080

aucacgaccu ccaucaccgg ccucuacgag caggagauca agaucaagug a              4131


<210>  2022
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2022
augaccacca agaaccgcga gaagaacaac aaguuccaca aggccagccc ccuguacacg       60

cucggccugg acaucgggac caccuccgug ggcuucaccg ccaucgacga ggcgcagcgg      120

ccgaugcgcg ccaaggggaa gacggucauc ggcgugcggc uguuccagga ggggcagagc      180

gccgugggcc gcaagaacuu ccggaccacc cgccggcgcc ucaagcggcg ccggcgccgg      240

cugaaccugc ucaagcaguu cuucuccgcg ccccugcgca agaucgaccc caccuucuuc      300

aagcggcuga aggccagcuc ccucagcccc gccgacaagc gccgguaccu ggccccgggc      360

cuguucccca acgggggcga caucaaguuc uaccagaagu accccacgau cuaccaccuc      420

cgccuggccc ugaugaccga gaagcggccc uucgaccucc gcgaggucua ccuggccauc      480

caccacaucg ugaaguaccg ggggaacuuc cugaacccgc uccccguguc cgcguuccgc      540

ggccgcaacc agcgguucga ggaccaguuc agcgagcuga accagcugua caagucccuc      600

gagggggaga gccccuucca guucgacgag aagaucuucu ccgagcugga caagcagcug      660

cucgucaacg acggccgcaa gaucgaccgg cagaagcagc uggccaagcg cguguucauc      720

aagaccaacg agaagcggau cgacagccac caccagaaga ucgccuccgc cuucagcaag      780

gccguguucg gcuacaaguu ccgccccgac guccugcuca acuccgaccc gaacgagagc      840

aucgacgagu ucaucaaguu cggggacgag gacuucgacg acaagcuggu gauccugcgg      900

gagucccuca acgcgaccca gaaggagauc cuggccguga cgcagcgcau cuacagccag      960

aucauccuga acgagaucgu ccccucccgg gagacccuca gcgaggccau ggugagccgc     1020

uaccagaagc accacuccga ccugcagcug cucaccgagg ccugcaagaa gaugaccaag     1080

gccaaggcgg cccggaucca ggccgccuac acggacuaca ucggcaaggg ggacggccac     1140

aagcuggugc acgaccaguu cgucgcccug aucaagaaga accucgaccc cagcgaggag     1200

ggccgcgacc uggcggagcg gcuggcccag gacgacuuca ugccccucca gcgcaccccg     1260

cugaaccggg ugauccccca ccagcugcag caggaggagc ucgaccgcau caucgagaac     1320

cagggggagu acuaccccuu ccugaagacc cugaacccca accggucccg cgccggcgug     1380

gccaacuacc agcucgacga gcuggucagc uuccggaucc cguacuacgu ggggccccug     1440

aucaccgcca cggaccagcu gcacaccucc ggcgcgaagu ucgccuggau gacccgccgc     1500

gccgccgggg agaucacccc cuggaacuuc gaccagaagg uggaccggcu cgcgagcgcc     1560

aaccaguuca uccgccagaa caugcccaag gacaaccacc uccuggaggc cgacguccug     1620

ccggccaacu cccugacgua ccagcgguac cuggugcucg acgagcugaa cgccgugaag     1680

gucaaccaca agagccugac cgugucccag aagcagcgca ucuucaacga ccucuucaag     1740

cggcagcgca ccgucaccgu gcugaaccug cagaacuacc ucaagagcau cgagaagcug     1800

cccacgcugc ccgugaucac cggcgucucc gacagcgagc gguucaagac cacccucucc     1860

acguaccugg acuucaagaa caucuucggc gggaaggugg acgacagcuc ccagaaggag     1920

aaguucgaga agaucauccu guacgcgacc aucuucgagg accacgccgu guacaaggcc     1980

aagcucgacc agaucgacug gcugggccag cagcagaaga ccgaccugcu cagcaagaac     2040

uaccaggggu ggggccagcu guccgagaag cugcuccugg ggcugaagaa ccaggccggc     2100

cagagcaucc ucgacgagcu guggcagacc aacaagcgcu uccgggagau caugaacgag     2160

gcccacuucg cgaagcagau cauggccucc aacgccgccc ugcuccgcaa gaagggccug     2220

gacgacgucc ugagcacggc cuuccucagc ccccagaaca agaaggcgau ccggcagacc     2280

guggaggugg ucgacgacau cgugaaggcc aucgggugcc cgccccagaa gaucucccug     2340

cacuucagca aggcccccgc cgacgcgauc cgccacgagc gggacaacgc caacaucauc     2400

cugcgcaccu accggaaccu cccggccaag augguggacg accagcugaa ggccgagcug     2460

caggccaccg cggagaagca ccagguccuc uccgacaagc uguaccugua cuucacccag     2520

cucggccgcg accuguacac gggggagaug cugaagauga gcgagcucga gaacgacaug     2580

aucgaccaca ucaugcccca gaccuucgcc uacgacgacu ccgugaacaa ccgggugcug     2640

gucagccaga accacuccag ccagcugggc aagaccuccg gggagaaguc cgacgccaag     2700

augauccgcu ucuggcaggu ccuccgggac aacaagcuga ucagcgagca gaaguaccag     2760

aaccugaccc ucgacagcga ccaccugucc aagagcacgc gcgagcagcu gcucaaccgg     2820

uaccuggugg agaagaaccc caucacccag uggguggccu ccgugcugac cgagaaguac     2880

ggcgacaacg gcacccgcgu gcucacgauc aacccccgca uggucuacca gaugcggcgc     2940

caguucaacc uggugaaccu gccggaggug aacgacuacg accacgggcu cgacgccuac     3000

cuggcggccu ucaucggcaa cuacgucgac cggcgcuacc cccggcugcg cagcuacuuc     3060

guguacgggg acuucuccag cagcaaggag aucguguucc ggcucaaccg cuucaacuuc     3120

cuguucgacc ugggccaggc cgagcccaac gagcucaccg acugggagac cggcgagaug     3180

aucugggaca aggccgccgc gaacuccguc cugcgggacg uguaccagua caaguucaug     3240

cugaucagcc aggccgugua cucccgccag ggggccaugu ucaaccagac caucuacccc     3300

gccagccagg cggccggccg gcagcucauc ccggucaaga agaaccgcgc cacgaagcug     3360

uacgggggcu acuccgggaa cguggacgcc uaccuggcca ucgugcggcu cggcggcccc     3420

aagagcgacc aguaccgcgu cguggggauc cccacccgga uggugggccg ccugcagucc     3480

gcgaugaagc agggggccga caccuacgcc aacacccugc acaagauccu ccagccccag     3540

uucacgaagc ggaagaagaa ccgccagacc ggcuacuacg aggagaccac cgagccguuc     3600

gccgucaucc uggacaaggu guacuaccgg cagcugauca ucgacgggca gcagaaguuc     3660

augcucggca gcuccacgua ccaguucaac gccaagcagc uggugcugag cucccgcgcg     3720

cgccagaucc ucagcggcga ggagcggcug accgacgaga acggguccca ggaccugauc     3780

gacgucuacg acgagauccu caccaagauc gaccgcuacu ucgagcugua cgaccagcgg     3840

cacuuccgcc agagccugca cgacggccgg aagcaguuca ucucccuccc cauccacaac     3900

guguaccgcg ccaaccggcu gaucgagcac gggaaguacg agacccugag ccagauccuc     3960

aacggccugc acgccaacac gaugaugacc aaccugcgcu accucgggau cuccagcccc     4020

uucggcaagu uccagucccc caacggcauc aagcugagca aggacgccgu gcuggucuac     4080

cagagcccgu ccgggcuguu cgagcggcgc gugcggcucc gcgaccugug a              4131


<210>  2023
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2023
augaacaaca agcccuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucaccg acgacuacaa ggugccgagc aagaagauga aggugcucgg gaacacggac      120

aagcacuuca ucaagaagaa ccugcugggc gcccuccugu ucgacgaggg gaccaccgcg      180

gaggaccgcc ggcugaagcg caccgcccgg cgccgguaca cgcgccggaa gaaccgccuc      240

cgguaccugc aggagaucuu caccgaggag auguccaagg ucgacaucag cuucuuccac      300

cgccuggacg acuccuuccu cgugcccgag gacaagcggg gcagcaagua ccccaucuuc      360

gccacccugg aggaggagaa ggaguaccac aagaacuucc ccaccaucua ccaccuccgc      420

aagcaccugg ccgacuccaa ggagaaggcc gacuuccggc ugaucuaccu ggcgcucgcc      480

cacaucauca aguaccgcgg ccacuuccug uacgaggaga gcuucgacau caagaacaac      540

gacauccaga agaucuucaa cgaguucauc uccaucuacg acaacacguu cgaggggagc      600

ucccugaacg gccagaacgc ccagguggag gccaucuuca ccgacaagau cagcaagucc      660

gcgaagcggg agcgcguccu caagcuguuc ccggacgaga aguccaccgg gcuguucagc      720

gaguuccuca agcugaucgu gggcaaccag gccgacuuca agaagcacuu cgaccuggag      780

gagaaggcgc cccuccaguu cagcaaggac accuacgacg aggaccugga gaaccugcuc      840

gugcagaucg gggacgacuu cgccgaccug uuccuggucg ccaagaagcu cuacgacgcc      900

auccugcugu ccggcauccu cacggugacc gaccccagca ccaaggcgcc ccuguccgcc      960

agcaugaucg accgguacga gaaccaccag aaggaccugg ccgcccucaa gcaguucauc     1020

aagaccaacc ugccggagaa guacgacgag guguucagcg accaguccaa ggacggcuac     1080

gccggguaca ucgacggcaa gacgacccag gaggccuucu acaaguacau caagaaccug     1140

cucagcaagc uggagggggc cgacuacuuc cuggacaaga ucgagcgcga ggacuuccuc     1200

cggaagcagc gcaccuucga caacggcucc aucccccacc agauccaccu ccaggagaug     1260

aacgccauca uccggcgcca gggcgagcac uaccccuucc ugcaggagaa caaggagaag     1320

aucgagaaga uccugaccuu ccgcaucccc uacuacgucg ggccgcuggc ccggggcaac     1380

cgcgacuucg cguggcugac gcggaacagc gaccaggcca uccgccccug gaacuucgag     1440

gagaucgugg acaaggcccg guccgccgag gacuucauca acaagaugac caacuacgac     1500

cucuaccugc ccgaggagaa ggugcugccc aagcacagcc ugcucuacga gaccuucgcc     1560

gucuacaacg agcugaccaa ggugaaguuc aucgcggagg ggcugcgcga cuaccaguuc     1620

cucgacuccg gccagaagca gcagaucgug acgcagcugu ucaaggagaa gcggaagguc     1680

accgagaagg acaucaucca guaccugcac aacguggaca gcuacgacgg gaucgagcuc     1740

aagggcaucg agaagcaguu caacgccucc cugagcaccu accacgaccu gcucaagauc     1800

aucaaggaca aggaguucau ggacgacucc aagaacgagg ccauccugga gaacaucgug     1860

cacacccuga cgaucuucga ggaccgcgag augaucaagc agcggcucgc ccacuacgcg     1920

agcaucuucg acgagaaggu caucaaggcc cugacccgcc ggcacuacac cggcuggggg     1980

aagcuguccg ccaagcucau caacggcauc uacgacaagc agagcaagaa gaccauccug     2040

gacuaccuga ucgacgacgg ggagaucaac cgcaacuuca ugcagcucau caacgacgac     2100

ggccuguccu ucaaggagau cauccagaag gcccaggugg uggggaagac gaacgacguc     2160

aagcaggucg ugcaggagcu gccgggcagc cccgccauca agaagggcau ccuccagucc     2220

aucaagcugg uggacgagcu ggugaagguc auggggcacg cgcccgagag caucgugauc     2280

gagauggccc gggagaacca gaccaccgcc cgcggcaaga agaacuccca gcagcgguac     2340

aagcgcaucg aggacgcccu caagaaccug gcccacgggc uggacagcaa cauccucaag     2400

gagcacccca ccgacaacau ccagcugcag aacgaccggc uguuccucua cuaccugcag     2460

aacggcaagg acauguacac ggggaagucc cuggacauca accagcucag cagcugcgac     2520

aucgaccaca ucaucccgca ggcguucauc aaggacgacu cccuggacaa ccgcgugcug     2580

accagcucca aggacaaccg gggcaagagc gacaacgucc ccucccucga gaucgugcag     2640

aagcgcaagg ccuucuggca gcagcugcug gacagcaagc ucaucuccga gcgcaaguuc     2700

aacaaccuga ccaaggccga gcggggcggg cuggacgagc gcgacaaggu gggcuucauc     2760

aagcggcagc ucgucgagac ccgccagauc accaagcacg uggcccagau ccuggacgcc     2820

cgguucaaca cggaggugac cgagaaggac aagaaggacc gcagcgucaa gaucaucacc     2880

cugaagucca accucgugag caacuuccgg aaggaguucc gccuguacaa ggugcgggag     2940

aucaacgacu accaccacgc gcacgacccc uaccugaacg ccgucguggc caaggccauc     3000

cucaagaagu accccaagcu ggagccggag uucguguacg gggacuacca gaaguacgac     3060

cugaagcgcu acaucucccg gaccaaggac cccaaggagg ucgagaaggc gacggagaag     3120

uacuucuucu acagcaaccu ccugaacuuc uucaaggagg aggugcacua cgccgacggc     3180

accaucguga agcgcgagaa caucgaguac uccaaggaca ccggcgagau cgccuggaac     3240

aaggagaagg acuucgccac caucaagaag guccugagcc ucccccaggu gaacaucgug     3300

aagaagacgg aggagcagac cgucgggcag aacggcgggc uguucgacaa caacaucgug     3360

uccaagaaga agguggucga cgccagcaag cugaccccca ucaagagcgg ccucuccccg     3420

gagaaguacg ggggcuacgc gcggcccacc aucgccuaca gcgugcuggu gaucgccgac     3480

aucgagaagg gcaaggccaa gaagcugaag cgcaucaagg agauggucgg gaucacggug     3540

caggacaaga agaaguucga ggccaacccc aucgcguacc ucgaggagug cggcuacaag     3600

aacaucaacc cgaaccugau caucaagcug cccaaguacu cccucuucga guucaacaac     3660

gggcagcggc gccugcuggc cagcuccauc gagcuccaga agggcaacga gcugaucgug     3720

cccuaccacu ucaccgcccu gcucuaccac gcccagcgga ucaacaagau cagcgagccc     3780

auccacaagc aguacgucga gacccaccag uccgaguuca aggagcugcu gaccgccauc     3840

aucagccucu ccaagaagua cauccagaag ccgaacgugg agagccugcu gcagcaggcg     3900

uucgaccagu ccgacaagga caucuaccag cucagcgagu ccuucaucag ccugcugaag     3960

cucaucuccu ucggggcccc cggcacguuc aaguuccugg gcguggagau cagccagucc     4020

aacguccgcu accagagcgu guccagcugc uucaacgcca cccugaucca ccagagcauc     4080

accgggcucu acgagacccg gaucgaccug uccaagcugg gcgaggacug a              4131


<210>  2024
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2024
auggacaaga aguacggcau cgggcuggac aucggcacca gcuccaucgg guggaccgug       60

gucgacaagu acggccacau cauccgcgug aaggggcaga cgggcaucgg cgugcggcug      120

uuccgcgagg ggcagaccgc cgccgagcgg cgcaccuucc ggaccacgcg ccggcgccug      180

agccggcgcc gguggcgccu gcggcuccug cgcgagaucu ucgacgagcc caucuccgcg      240

aucgacgaga acuucuucgc ccggcagaag gccagcuccc uggccccggg cgacgcccgc      300

uaccagcagg cguuccagcu guucaacgac cggaccgacc aggccuucua cugcaaguac      360

cccaccaucu accaccuccg ccaggcccug augaccgaga gccggcaguu cgacauccgc      420

gagaucuacc ucgcccugca ccagaucauc aaguaccggg ggcacuuccu gcucggcggg      480

gcggccaagg acuuccaguc cgacaagcug gaccuggagg ccuacuucac gaccaucaac      540

gcccagcucg ccggcaucua cagcgacggc uccguccagc ugcccacccu ggacuggaac      600

caggcgugcc aggagcucac cgacacgagc cgcacccagu ccgaccgcaa gaacuggcug      660

gugaaggagc ucaagagcua cggggacucc cagcucaagc ccaucuacac ccagaucgcc      720

aacgccaucc ugggcuucaa gaccgaccug gccaagauca uggggaucaa cgacccggag      780

aaggcccuca gcuucaaccu ggacgcgcug gacgaccacc gggacgacgu ggccgagcuc      840

cugacggacg agcagauccc ccuggucaac cuccuggcca agcugcacac cgcccucgag      900

cuggccgcga uccugcccga cggccagacc cucuccggca agauggugga gaucuacgag      960

acccaccgca agcagcugaa gacguacaag gccuucaucc ggcagcugcc cgaccgccag     1020

cagcggcucg cccugaagca ggccuacgac cacuacaucg accaggggcu gaaccgcgag     1080

gccuucuaca gcgaggugaa ggcgauccuc gagaagcaga agccgcugac cgccaccggc     1140

caggccauga agaaggccau ggaccagaac gcguuccugc ucaagcagcg gaccaagacg     1200

aacaucgcca uccccuacca gguccagcag caggagaugg accagaucau cgccaaccag     1260

uccaaguacu accccuggcu ggccaccccc aacccgguga agucccgccg ggugaucgcc     1320

cccuacaagc uggacgagcu cgucugcuuc cgcgugaacu acuacguggg gccccugguc     1380

caguccaagc ccggcgaggc gaccaaggag gccaaguucg ccuggaugac ccggaaggac     1440

ccgcagagca cgguggccau cacccccugg aacuucgacc agcaggucga ccgccaggcc     1500

agcgcgaacg ccuucaucca gcggaugaag accaccgaca cguaccugcu gggggaggac     1560

gugaucccca agaacucccu ccuguaccag aaguacgagg ugcugaacga gcucaacaac     1620

gugcgcguca acggcgacug gcuggacccc cagcggaagc aguaccuguu caagaagcug     1680

uucauggacc agccgcagag cguggugacc gucaaggccc ugcagaagac ccuggugguc     1740

aucggcgccu gccccacccu ccccacggug gaggggcugg ccaaccccaa gcgcuuccug     1800

ucccagcuca ccaccuacca cgacuucugc aagcugcugc cggacgacau ccagcgggcg     1860

gaccgccagg ccgacaucga gaagaucauc aacuggagca ccaucuucga ggacaccgcc     1920

aucuucaagg agaagcuccg ggacaucaag uggcuguccg ccgaccagau ccgccgccug     1980

agcucccggc cccgcuaccg gggcuggggg cgcuucagcg cgaagcuccu gugcggccug     2040

cacgaccggc aggggaacac ggugauggac gagcucuggc agaccucccg caacuucaug     2100

cagaucaucc ggcagagcga cuuccagacc gccaucgccg ccgucaacca gcaggaccug     2160

ggccgccagg accugcagcg ggugaucgac gaccucuaca ccagccccca gaacaagaag     2220

gccauccgcc aggugcuggc ggucgugacg gacauccaga aggccaugca cggcaaggcc     2280

cccagcuggc uguucgugga ggucgccaag ggggcccaga agaacccgca gcggacccag     2340

ucccgccaga gcaagcucgc ggaggccuac cgggaguccg cccgcgagcu gguggacgag     2400

gccgugaagg agcagcugcu cacccugccc gacgaccggc agaccuucag cgaccgccug     2460

guccucuacu uccagcagaa cggccgggac aucuacacgg gggagccccu ggacuucgag     2520

cgccugucca acuaccacau cgaccacauc aucccgcagg ccguggugaa ggacgacagc     2580

cucgacaacc ggguccugac cuccaaccag gcgaaccugg agaagggcgc ccacgugccc     2640

accaucuucc gcgacaagca ggagcccgug uggcaggagc ucuaccgggu cgggcugauc     2700

agccaccgca agcugcgcaa ccuccugacc acgcccgacg accuggacga ccggggccgc     2760

gccaccggcu ucaucgcccg gcagcucgug gagacccgcc aggugaucaa gcuggucaug     2820

gaggugcuga gccaguacua cgacaccacg gagaccgagc ucaucuccau caaggcgggg     2880

cugagccacc aguuccggca ggagcugaac cucccgaagc ugcgcgcccu gaacgacuac     2940

caccacgccc aggacgccua ccucgccgcg cggaucggca ccuaccugcu gcagcgcuac     3000

cccaaccuca agcgguucuu cguguacggg gacuucaaga agacccacuu cgaccugcag     3060

cgccgguuca acuucaucgg cggcaugauc ucccgcgaga agcagcccua caaccugauc     3120

aagaaccccg ucacgcacca ggugaucugg gacgaggaga uggagaucgg ggacugccag     3180

aagaagaacc cggagcagcc caagcacgac gccaccguga agaugaugaa ccucaccaag     3240

aucaugaacc ugaagcacau ccuggucacc cacgaggugu acgagaacca cggcgcccuc     3300

uucaaccaga cgaucuaccc cgccagccag gcccacaaga agaagcugau cccccagaag     3360

accgagaagc cgaucgagcu guacgggggc cacuccagcg agcaggcggg guacauggcc     3420

aucauccggc uccugggcaa gcgccccggc uacaagguga ugcggguccc cgugcgcuac     3480

gugucccagc ugcagcacca ggacgucgcc gagcagaaga agcuccugua caagaucuuc     3540

gugagcgagc ugaccucccg gaacaaggug aagcccuucg aggucguggu gccgcacguc     3600

ugccucaacc agcccgugga ggacaugauc aaggggcagu ggcaccgcuu cgcccugcgg     3660

accaacggcu acuaccagaa ccagcagcag cugauccuca gccugguggc ccagcgcacg     3720

cuggcggggu acaagcccaa ggacgacgag cucauggccg ucuaccacga gaucugcaag     3780

caggucaagu acuacuuccc ccuguacaag augaacaagu uccagucccg ccuguucgcc     3840

gccgaggagc uccugcagaa gcggagcacc gugaacuccu ucagcaucac caucggcaag     3900

aacgggaagg agaacaagac ccugaaccuc ggcaagcgcg agcugcugcu ccggcugcug     3960

ggcgggcucc acgccaacgc gacgcgcgug gaccugaagg acaucggcug caccgggcag     4020

uucggccagc uccaggugaa gaacgggauc aagcucaccg agcaggccgu gcugauccac     4080

gaguccccga ccggccuguu ccaccggcgc guccggcuga acgaccucug a              4131


<210>  2025
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2025
augaccaaga agaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucacgg acgacuacaa ggugcccgcc aagaagauga aggugcucgg gaacaccgac      120

aagaaguaca ucaagaagaa ccugcugggc gcgcuccugu ucgacagcgg ggagaccgcc      180

gaggccaccc gccugaagcg gacggcccgc cggcgcuaca cccggcgcaa gaaccggcuc      240

cgcuaccugc aggagaucuu cgccgaggag augaccaagg ucgacgaguc cuucuucuac      300

cggcuggacg agagcuuccu caccacggac gagaaggacu ucgagcgcca cccgaucuuc      360

ggcaacaagg cggacgagau caaguaccac caggaguucc ccaccaucua ccaccuccgg      420

aagcaccugg ccgacuccag cgagaaggcc gaccugcgcc ugguguaccu ggcccucgcg      480

cacaugauca aguuccgggg ccacuuccug aucgaggggg agcugaacgc cgagaacacc      540

gacgugcaga agaucuucgc cgacuucguc ggcguguacg accgcaccuu cgacgacucc      600

caccucagcg agaucacggu ggacgccgcc uccauccuga ccgagaagau cagcaagucc      660

cggcgccugg agaaccucau caaguacuac cccaccgaga agaagaacac ccuguucggg      720

aaccugaucg cgcucgcccu gggccugcag cccaacuuca agaugaacuu caagcucagc      780

gaggacgcca agcugcaguu cuccaaggac agcuacaacg aggaccugga ggagcuccug      840

gggaagaucg gcgacgacua cgccgaccug uucacguccg ccaagaaccu cuacgacgcg      900

auccugcuga gcggcauccu caccgucgac gacaacucca ccaaggcccc gcugagcgcc      960

uccaugauca agcgguacgc cgagcaccac gaggaccugg agaagcucaa ggaguucauc     1020

aaggccaaca agagcgagcu guaccacgac aucuucaagg acgagaccaa gaacggguac     1080

gcgggcuaca ucgagaacgg ggugaagcag gacgaguucu acaaguaccu gaagaacacg     1140

cucagcaaga ucgccggcuc cgacuacuuc cuggacaaga ucgagcgcga ggacuuccug     1200

cggaagcagc gcaccuucga caacggcagc aucccccacc agauccaccu ccaggagaug     1260

cacgccaucc ugcggcgcca gggggacuac uaccccuucc ugaaggagaa ccaggaccgc     1320

aucgagaaga uccucaccuu ccggaucccc uacuacgugg gcccgcuggc ccgcaaggac     1380

ucccgguuca gcugggccga guaccacucc gacgagaaga ucacccccug gaacuucgac     1440

aaggucaucg acaaggagaa guccgcggag aaguucauca cgcgcaugac ccugaacgac     1500

cuguaccucc ccgaggagaa ggugcugccc aagcacagcc acguguacga gaccuacgcc     1560

gucuacaacg agcugaccaa gaucaaguac gugaacgagc aggggaagga cagcuucuuc     1620

gacuccaaca ugaagcagga gaucuucgac cacguguuca aggagaaccg gaaggucacg     1680

aaggagaagc uccugaacua ccugaacaag gaguucccgg aguaccgcau caaggaccuc     1740

aucggccugg acaaggagaa caagagcuuc aacgccuccc uggggaccua ccacgaccuc     1800

aagaagaucc uggacaaggc cuuccuggac gacaagguga acgaggaggu gaucgaggac     1860

aucaucaaga cccucacccu guucgaggac aaggacauga uccacgagcg gcugcagaag     1920

uacagcgaca ucuucacggc ggaccagcuc aagaagcugg agcgccggca cuacaccggc     1980

uggggccgcc uguccuacaa gcucaucaac gggauccgga acaaggagaa caacaagacc     2040

auccuggacu accugaucga cgacggcagc gccaaccgca acuucaugca gcucaucaac     2100

gacgacaccc ugcccuucaa gcagaucauc cagaaguccc aggucguggg ggacguggac     2160

gacaucgagg ccgucgugca cgaccugccc ggcagccccg ccaucaagaa ggggauccuc     2220

caguccguca agaucgugga cgagcuggug aaggucaugg gcgacaaccc ggacaacauc     2280

gugaucgaga uggcccggga gaaccagacg accaaccgcg gccggagcca gucccagcag     2340

cgccugaaga agcuccagaa cagccugaag gagcugggga gcaacauccu caacgaggag     2400

aagcccuccu acaucgagga caagguggag aacagccacc uccagaacga ccagcuguuc     2460

cuguacuaca uccagaacgg caaggacaug uacaccgggg acgagcugga caucgaccac     2520

cuguccgacu acgacaucga ccacaucauc ccccaggcgu ucaucaagga cgacagcauc     2580

gacaaccggg uccucaccuc cagcgccaag aaccgcggca aguccgacga cgugcccagc     2640

cuggacaucg ugcgggcccg caaggccgag uggguccgcc uguacaaguc cgggcucauc     2700

agcaagcgga aguucgacaa ccugaccaag gccgagcgcg gcggccugac ggaggcggac     2760

aaggccgggu ucaucaagcg gcagcucgug gagacccgcc agaucaccaa gcacguggcc     2820

cagauccugg acgcccgguu caacaccgag uccgacgaga acgacaaggu cauccgcgac     2880

gugaagguga ucacgcucaa gagcaaccug gucucccagu uccggaagga cuucgaguuc     2940

uacaaggugc gcgagaucaa cgacuaccac cacgcccacg acgcguaccu gaacgccgug     3000

gucggcaccg cccugcucaa gaaguacccg aagcuggcca gcgaguucgu guacggggag     3060

uacaagaagu acgacgugca caagcugauc gcgaagucca gcgacgacca cuccgagaug     3120

ggcaaggcca ccgccaagua cuucuucuac agcaaccuca ugaacuucuu caagcggguc     3180

auccgcuaca gcaacggcaa ggugaucgug cggcccgucg uggaguacuc caaggacacc     3240

gaggacaucg ccugggacaa gaaguccaac uuccgcacga ucugcaaggu gcugagcuac     3300

ccccagguca acaucgugaa gaagguggag acccagaccg ggggcuucag caaggagucc     3360

auccugccca agggggacag cgacaagcuc aucccgcgga agaccaagaa ggccuacugg     3420

gacacgaaga aguacggcgg guucgacucc cccaccgucg cguacagcgu guucgugguc     3480

gccgacgugg agaagggcaa ggccaagaag cugaagaccg ugaaggagcu ggucggcauc     3540

uccaucaugg agcgcagcuu cuucgaggag aaccccgugg aguuccucga gaacaagggg     3600

uaccacaaca uccgggagga caagcugauc aagcugccca aguacucccu cuucgaguuc     3660

gagggcggga agcgccggcu gcuggccagc gccuccgagc uccagaaggg caacgagaug     3720

gugaucccgg ggcaccuggu caagcugcuc uaccacgcgc agcgcaucaa cagcuucaac     3780

uccaccaagu accuggacua cgugagcgcc cacaagaagg aguucgagaa ggugcugucc     3840

ugcgucgagg acuucgccaa ccucuacgug gacguggaga agaaccugag caagauccgg     3900

gccgucgccg acuccaugga caacuucagc aucgaggaga ucagcaacuc cuucaucaac     3960

cugcucacgc ugaccgcgcu gggcgccccc gccgacuuca acuuccucgg cgagaagauc     4020

ccccgcaagc gguacaccag caccaaggag ugccugaacg ccacgcugau ccaccagucc     4080

aucaccgggc ucuacgagac ccgcaucgac cugagcaaga ucggcgagga guga           4134


<210>  2026
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2026
augaagaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gccgagcaag aagaugaagg ugcucgggaa cacggacaag      120

cacuucauca agaagaaccu gaucggcgcc cugcuguucg acgaggggac caccgcggag      180

gaccgccggc ugaagcgcac cucccggcgc cgguacacgc gccggaagaa ccgccugcgg      240

uaccuccagg agaucuucag cgaggagauc uccaagcugg acagcuccuu cuuccaccgc      300

cuggacgaca gcuuccucgu ccccgaggac aagcggggcu ccaaguaccc caucuucgcc      360

acccuggagg aggagaagga guaccacaag aaguucccca ccaucuacca ccuccgcaag      420

caccucgccg acagcaagga gaagaccgac cugcggcuga ucuaccucgc ccuggcccac      480

augaucaagu accgcggcca cuuccuguac gaggaguccu ucgacaucaa gaacaacgac      540

auccagaaga ucuucaacga guucaucagc aucuacgaca acacguucga gggguccagc      600

cucuccggcc agaacgccca gguggaggcc aucuucaccg acaagaucag caaguccacc      660

aagcgggagc gcgugcugaa gcuguucagc gacgagaagu ccaccgggcu guucagcgag      720

uuccugaagc ugaucgucgg caaccaggcc gacuucaaga agcacuucga ccucgaggag      780

aaggccccgc ugcaguucag caaggacacg uacgacgagg accuggagaa ccuccugggg      840

cagaucggcg acggcuucgc ggaccuguuc cucguggcca agaagcugua cgacgccauc      900

cugcucuccg ggauccugac cgugaccgac cccagcacca aggcgccccu guccgccagc      960

augaucgagc gguacgagaa ccaccaggag gaccucgcgg cccugaagca guucaucaag     1020

aacaaccugu ccgagaagua cgccgagguc uucagcgacc aguccaagga cggcuacgcc     1080

ggguucaucg acggcaagac gacccaggag gccuucuaca aguacaucaa gaaccuccug     1140

agcaagcugg agggcgcgga cuacuuccuc aacaagaucg agcgcgagga cuuccugcgg     1200

aagcagcgca ccuucgacaa cggguccauc ccccaccaga uccaccucca ggagaugaac     1260

gccauccucc ggcgccaggg cgagcacuac cuguuccuga aggagaaccg cgagaagauc     1320

gagaagaucc ucgccuuccg gaucccguac uacguggggc cccuggcccg cggcaaccgg     1380

gacuucgccu ggcugacccg caacagcgac caggcgaucc ggcccuggaa cuucgaggag     1440

guggucgaca aggccuccag cgccgaggac uucaucaaca agaugacgaa cuacgaccug     1500

uaccuccccg aggagaaggu gcugccgaag cacucccugc ucuacgagac cuucgccgug     1560

uacaacgagc ugaccaaggu caaguucauc gccgaggggc ugcgcgacua ccaguuccuc     1620

gacagcggcc agaagaagca gaucgugaac cagcuguuca aggacaagcg gaaggugacc     1680

gagaaggaca ucauccacua ccugcacaac gucgacggcu acgacgggau cgagcucaag     1740

ggcaucgaga agcaguucaa cgcgucccug agcacguacc acgaccugcu caagaucauc     1800

aaggacaagg aguucaugga caaccccaag aacggggaga uccuggagaa caucauccac     1860

acccugacca ucuucgagga ccgcgagaug aucaagcagc ggcucgccca guacgacacc     1920

cuguucgacg agaaggugau caaggcccug acgcgccggc acuacaccgg cugggggaag     1980

cucuccgcca agcugaucaa cggcauccgc gacaagcaga ccggcaagac cauccuggag     2040

uaccucaucg acgacgggga cugcaaccgg aacuucaugc agcugaucaa cgacgacggc     2100

cugagcuuca aggagaucau ccagaaggcc cagguggucg ggaagacgga cgacgugaag     2160

caggucgugc aggagauccc cggcuccccc gcgaucaaga aggggauccu ccagagcauc     2220

aagaucgugg acgagcuggu caaggugaug ggccacaacc cggagagcau cgugaucgag     2280

auggcccgcg agaaccagac caccgccaag ggcaagaaga acucccagca gcgguacaag     2340

cgcaucgagg acgcccugaa gaaccucgcg cccgggcugg acagcaacau ccugaaggag     2400

aaccccaccg acaacaucca gcuccagaac gaccggcugu uccuguacua ccuccagaac     2460

ggcaaggaca uguacacggg gaaggccauc gacaucaacc agcuguccaa cuacgacauc     2520

gaccacauca ucccccaggc cuucaucaag gacgacagcc uggacaaccg cguccucacc     2580

uccagcaagg acaaccgggg caaguccgac aacgugccga gcaucgaggu gguccagaag     2640

cgcaaggccu ucuggcagca gcugcuggac uccaagcuca ucagcgagcg caaguucaac     2700

aaccugacca aggccaagcg gggcgggcug gacgagcgcg acaagguggg cuucaucaag     2760

cggcagcucg uggagacccg ccagaucacc aagcacgucg cgcagcugcu ggacacgcgg     2820

uucaacaccg aggugaacga ggagaaccag aagauccgca ccgugaagau caucacccuc     2880

aaguccaacc uggucagcaa cuuccggaag gaguucgggc uguacaaggu gcgcgagauc     2940

aacgacuacc accacgccca cgacgccuac cucaacgccg uggucgccaa ggcgauccug     3000

aagaaguacc ccaagcugga gcccgaguuc guguacggcg acuaccagaa guacgaccuc     3060

aagcgguaca uccugaaguc caaggccagc aacacgaucg acaaggccac cgagaaguac     3120

uucuucuacu ccaaccugcu caacuucuuc aaggagaagg ugcgcuacgc cgacgggacc     3180

aucaagaagc gggagaacau cgaguacagc aacgacaccg gcgagaucgc guggaacaag     3240

gagaaggacu ucgccacgau caagaagguc cugucccugc cccaggugaa caucgugaag     3300

aagaccgagg agcagaccgu cggccagaac gggggccucu ucgacaacaa caucgugagc     3360

aagaagaagg uggucgacgc cagcaagcug aucccgauca aguccagccu gucccccgag     3420

aaguacgggg gcuacgcccg ccccaccauc gccuacagcg ugcucgugau cgcggacauc     3480

gagaagggga agggcaaggc caagaagcug aagcggauca aggagaucgu cggcaucacg     3540

auccaggaca agaagaaguu cgaguccaac cccgugaccu accuggagga gugcggguac     3600

aagaacauca acagcaaccu caucaucaag cugccgaagu acucccuguu cgaguucaac     3660

gacggccagc gccggcuccu ggccagcucc aucgagcugc agaaggggaa cgagcucauc     3720

cugcccuacc accugaccgc ccuccuguac cacgcccagc gcaucaacaa gaucagcgag     3780

cccauccaca agcaguacgu ggaggcgcac cagaacgagu ucaaggagcu gcucaccacg     3840

aucaucuccc ugagcaagaa guacauccag aagccgaacg ucgagcugcu ccugcagcag     3900

gccuucgacc aggccgacaa ggacaucuac cagcuguccg agagcuucau cucccuccug     3960

aagcugacca gcuucggcgc ccccggggcc uucaaguucc ucggcgugga gaucucccag     4020

agcuccgugc gguacaagcc caacagccag uuccuggaca ccacccugau ccaccagagc     4080

aucacgggcc ucuacgagac ccgcaucgac cuguccaagc ugggggagga cuga           4134


<210>  2027
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2027
augaacaaca agcccuacag caucggccug gacaucggga ccaacuccgu gggcuggagc       60

gucaucaccg acgacuacaa ggugccgucc aagaagauga aggugcucgg gaacacggac      120

aagcgcuuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcc      180

gaggcgcggc gccugaagcg gaccgcccgc cggcgcuaca cgcggcgcaa gaaccggcug      240

cgcuaccucc aggagaucuu cgccgaggag augagcaagg ucgacuccag cuucuuccac      300

cggcuggacg acuccuuccu gauccccgag gacaagcgcg gcagcaagua ccccaucuuc      360

gccacccucg uggaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgg      420

aagcagcugg ccgacuccaa ggagaaggcg gaccuccgcc ugaucuaccu ggugcucgcc      480

cacaugauca aguaccgggg ccacuuccug uacgaggagg ccuucgacau caagaacaac      540

gacauccaga agaucuucaa cgaguucauc agcaucuacg acaacaccuu cgaggggucc      600

agccuguccg gccagaacgc ccaggucgag acgaucuuca ccgacaagau cagcaagucc      660

gcgaagcgcg agcgggugcu caagcuguuc ccggacgaga aguccaccgg gcuguucagc      720

gaguuccuca agcugaucgu gggcaaccag gccgacuuca agaagcacuu cgaccuggag      780

gagaaggcgc cccuccaguu cagcaaggac accuacgacg aggaccugga gaaccugcuc      840

gagcagaucg gggacgacuu cgccgaccug uucgucgccg cgaagaagcu guacgacgcc      900

auccuccugu ccggcauccu gacggugacc gaccccagca ccaaggcgcc ccucagcgcc      960

uccaugaucg agcgcuacga gaaccaccag aaggaccugg ccgugcugaa gcaguucauc     1020

aagaccaacc ucccggagaa guacgucgag guguucagcg accaguccaa ggacggcuac     1080

gccggguaca ucgacggcaa gacgacccag gaggccuucu acaaguacau caagaaccug     1140

cugagcaagu ucgacggggc cgacuacuuc cucgacaaga ucgagcggga gguguuccug     1200

cgcaagcagc ggaccuucga caacggcucc aucccccacc agauccaccu ccaggagaug     1260

aacaccaucc ugcgccgcca gggcgagcac uaccccuucc ugaaggagaa caaggagaag     1320

aucgagaaga uccugacguu ccggaucccc uaccacgucg ggccgcucgc ccgcggcaac     1380

cgggacuucg ccuggcugac ccgcaacagc gaccaggcga uccggcccug gaacuucgag     1440

gagaucgugg acaaggccuc cagcgccgag gacuucauca acaagaugac caacuacgac     1500

cuguaccucc ccgaggagaa ggugcugccc aagcacuccc ugcuguacga gaccuucgcc     1560

gucuacaacg agcucacgaa ggugaaguuc aucgccgagg ggaugcgcga cuaccaguuc     1620

cuggacagcg gccagaagaa gaagaucauc aaccagcugu ucaagaagaa gcggaaggug     1680

accgaggacg acaucaucga cugccuccag aagaucgaca acuacgacgg gaucgagcug     1740

aagggcaucg agaagcaguu caacgcgucc cugagcaccu accacgacau ccucaagauc     1800

auccgcgaca aggaguucau ggacgacccg aagaacggcg acauccugga gaacgucauc     1860

cacacccuga cgaucuucga ggaccgggag augaucaagc agcgccucgc ccaguacaac     1920

uccaucuucg acgagaaggu gaucaaggcc cugagccgga agcacuacac cggguggggc     1980

aagcuguccg ccaagcucau cgacgggauc cgcgacaagc agaccggcaa gaccauccug     2040

gacuaccuga uggacgacgg caagagcaac cggaacuuca ugcagcucau caaggacgac     2100

gagcuguccu ucacggagau cauccagaag gcccaggugg ucggggacac cgacaaccug     2160

cgccaggugg uccagaaccu ccccggcagc cccgccauca agaaggggau ccugcagucc     2220

aucaagaucg uggacgagcu ggugaagguc augggccacg cccccgagag caucgugauc     2280

gagauggccc gggagaacca gaccaccgcc cgcgggaaga agaacagcca gcagcgguac     2340

aagcgcaucg aggacucccu caagaaccug gcgagcggcc uggacuccaa cauccucaag     2400

gagaacccga cggacaacau ccagcugcag aacgaccggc uguuccucua cuaccugcag     2460

aacgggaagg acauguacac cggcgaggcc cugaacauca accagcucag cuccuacgac     2520

aucgaccaca ucauccccca ggccuucauc aaggacgaca gccuggacaa ccgcgugcug     2580

accuccagca aggacaaccg cggcaagucc gacaacgucc ccagccucga ggugguggag     2640

aagaugaagg ccuucuggca gcagcugcug gacuccaagc ucaucagcga gcggaaguac     2700

aacaaccuga ccaaggccga gcgcgagcgc gggggccuga acgagcucga caaggucggg     2760

uucaucaagc ggcagcuggu ggagacccgg cagaucacga agcacguggc gcagauccug     2820

gacgcccgcu ucaacaagga ggucaccgag aagaacaaga agaaccggaa cgugaagauc     2880

aucacccuca aguccaacau ggugagcaac uuccgcaagg aguucggccu guacaagguc     2940

cgggagauca acgacuacca ccacgcccac gacgccuacc ugaacgccgu gguggcgaag     3000

gccauccuca agaaguaccc caagcuggag ccggaguucg ucuacggcga cuaccagaag     3060

uacgaccuga agcgcuacau cucccggagc aaggacccca aggaggugga gaaggccacc     3120

gagaaguacu ucuucuacuc caaccuccug aacuucuuca aggaggaggu gcacuacgcc     3180

gacgggacga ucgucaagcg cgagaacauc gaguacagca aggacaccgg cgagaucgcg     3240

uggaacaagg agaaggacuu cgccaccauc aagaaggugc ugagccuccc ccaggugaac     3300

aucgucaaga agaccgagga gcagacggug gggcagaacg gcgggcuguu cgacaacaac     3360

aucgugucca agaagaaggu cguggacgcc agcaagcuga uccccaucaa guccggccuc     3420

agcccggaga aguacggcgg guacgcccgg cccaccaucg ccuacuccgu gcuggucacc     3480

uacaagcaga acaacaagau caagaacacc auggugggca uccccgugcu gacgaagcuc     3540

gaguucgagc gcgacccgaa ccuguaccug cggaaccucg ggaucaacaa cgucagccac     3600

gugaucaagc uggugaagua caccguccug gaguucaagc acaaguacgg caccuaccgc     3660

cgguucaucg uguccaacca ggagcucaag cgcgcgaacc agaucuucgu gaccacgaag     3720

cagaugaagc ugaucagcca guacaccacc cuggacaaga acgagcacga cucccuccug     3780

aagaacaacc agcagcugau ccagagcauc uggaaccagu uccucgaguu caugcuggcc     3840

accgaccugg ucaacaagaa gcaguacgac gagcucaucc ugacgaacaa ccagaccauc     3900

gaggagugcc ggaacaucau gaaccgcgug aucaccaccc uguccuucac gaacaucaac     3960

gccgggcaga agaagaucau cuucgacgac acccucagcc ugcccaucaa gcgguggcag     4020

uucaccgacu ccgacaagau ggugaagaag aucaucgaga gcacccugau ccaccagucc     4080

aucacgggcc ucuacgagac ccgcaucgac cugagcaagc ugggcgagga cuga           4134


<210>  2028
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2028
auggagagca accacgacgg cgagcagguc aaguacaccc uggggcucga caucggcacc       60

uccagcaucg gguacgccgc caucgacaag caccagaagc ccauccgcgc gaagggcaag      120

cacgugaucg gggugcggcu guuccaggag ggccagacgg ccgccgaccg ccgggccuuc      180

cgcaccaccc ggcgccggcu gaagcgccgg aaguggcgcc ucaaccugcu gaaccgccuc      240

uucgacccgu accuguccga gguggacccc aacuuccugc cccggcuccg gcagagcaac      300

cuguccccca aggacgacaa caagcacuuc aacggcagca cccuguuccc ggacaagacg      360

gacgccgcgu ucuaccgcca guaccccacc auguaccacc uccgguacgc ccugaugacc      420

gagaagcgca aguucgacau ccgggaggug uaccuggcca uccaccacau cgugaaguac      480

cgcgggaacu uccucaacuc cgccaccgug gacagcuuca agacguccaa caucgacuuc      540

accagccagu ucgaccggcu gaacgagcug uaccgccagg ucauccucga cgagcccuuc      600

cagaucaaca uggaccaggu ggacgagaug accaacaagc ugcuggacaa cgacgcgcuc      660

aagcuggaca cccagaagca ggucgccaag cugcuccccg ugaucuacaa cgacaagccg      720

aucgacaagc aguacacgcg gaucgccacc gaguucucca aggccauccu gggcuacaag      780

accaagcugg acgugauccu caaccuggac acccagaacg ccaaggacug ggugauccgc      840

cuggacgacg aggacaucga cgacaagcuc cccgcgcugg ucgagaaccu ggacgagagc      900

cgccaggaga ucgugacgau cauccgggac cucuacgccc agaucacccu gaacgccauc      960

gugcccaagg ggaagucccu gagcgagucc auggucgaca aguacaacga ccacaaggac     1020

caccucgacc ugcugauggg ccucauccac gagcuggggg acgacagcgc caagggcauc     1080

aagcugcgcg aggccuacac ccaguacgug ggcaaguccg acgacaagac ccucaaccag     1140

gacgacuucu acgcggccau caagaagaac cuggacgagg acagcaagcg gucccccaag     1200

auccagcgcc ugaucgagca ggccagcuuc augccgaagc agcggacgag cgccaacggg     1260

gugauccccc accagcucca ccagcuggag cuggaccgca ucaucgagaa ccagggccgg     1320

uacuaccccu uccucaagaa gcccaacccg aacacccaca agcccaacgg gggcaaguac     1380

aagcuggacg agcuggucgc cuuccgcauc cccuacuacg ugggcccccu gaucaccaag     1440

cgggaccagg agaagaccuc cggggcggac uucgccugga ugauccgcaa gccgggccag     1500

gagggggaga ucacgcccug gaacuucgac cagaaggugg accggauggc cagcgccaac     1560

cgcuucaucc ggcgcaugac caccaaggac uccuaccucg ucgccgagga cgugcugccc     1620

gacgcgagcc ugaucuacga gaaguucaag gcccucaacg agcugaacau gcugaaggug     1680

aacgacuccc ggcucagccc cucccagaag caggaccugu ucaacaaccu guucaagaag     1740

cagaagacca uccgcacgaa gaagcuccag accuacaucc ggaccaacug ggagcugccg     1800

agcgucauga ucuccggccu ggccgacccc gacaaguuca acagcucccu cagcaccuac     1860

aucgacuucc gcgacaucuu cggggagaag guggacgacc ccaaccggca ggccgacuac     1920

gaggagauca ucgagugguc cacggucuuc gaggaccgca agaucuacca ggcgaagcug     1980

aaccagcugg acuggcucac cgagccccag cgcaaggccc ugcugaccaa gcgguacacc     2040

ggcuggggcc gccucagcaa gaagcugcug acggggcuca aggaccagaa cggcgacucc     2100

auccuggacc agcuguggaa gaccaacgac aacuucaugc agauccagag ccgggacgag     2160

uucgccaagc agauccacga cgagaacgcc aagcaguucc acgaggccaa caacguggac     2220

gacauccucg acgacgcguu caccuccccg cagaacaaga aggccauccg ccagguggac     2280

aaggucgugc aggacguggu caaggccgug ggguacgccc ccgacaagau cgccaucgag     2340

uucacccgga gccuggagaa caacccccag cgcaccgugu cccggcagcg ccagcugcag     2400

gcggccuaca agaacauccc gcgggagauc augaaguccg gccucauggc cgagcugggg     2460

agcgucgccg acagcaagaa ggugcugagc gacaagcucu accuguacuu cacgcagaug     2520

ggcaaggaca uguacgagga caagaccauc aacaucgaca accugcccaa cuacgacauc     2580

gaccacaucc ucccccaggc cuucaucaag gacgacucca ucgacaaccg cgugcugauc     2640

agcaaggagg agaacgcgcg gaaguccaac cgcguccccc ugaaguucuu cggcgccaag     2700

cggauggggu ucuggaagga ccuccugaac aacggccuga ucagcaaccg caaguuccag     2760

aaccucacca ccgacccgga gaagaucucc aaguacagcg ccaagggguu cauccaccgg     2820

cagcuggugg agacguccca ggugauccgc cuggucgcca acauccucgg caacgaguac     2880

agccaggcgg ggaccaccau caucgaggug uccgccaaga ugaaccggca gcugcgcgag     2940

gacuuccacc ugaucaagaa ccggaacgug aacgacuacc accacgccau ggacgccuac     3000

cucaccgccu acgucggcga cuaccuguac cugcgcuacc ccaagcuccg gcccuacuuc     3060

guguacggcg acuucaagaa ggcggaggac caggaccuga acauccgcaa cuucaacuuc     3120

cugcacgacc ucaaggacaa cgagagccug gacaagaucg uggacuccga gacgggggag     3180

aucgucuggg agaagcacgg cagcgugaag cagcugaaga agguguacca cuacaaguuc     3240

augcucgucu cccaggaggu guacacccgc caggacgccc uguucaacca gaccaucuac     3300

cccgccagcg acgccgacaa gcggaagcug aucccgauca agaacuccaa gcccguggac     3360

gucuacgggg gcuacagcgg caacaucgac gccuacaugg cgaucgugcg cgugcacggg     3420

aagaaggagg acaacuacaa ggucgugggc auccccaugc gggccgugac caagcucaag     3480

aaggccgaga aggaggggca cgacagcuac cugaacgccg uccacgaggu gcugaagccc     3540

caguucacga agaagaagaa gaaccgcaag accggcgaga ucaccgagac cguggacgag     3600

uucgacgucc uccuggggaa gguguacuac cggcagcuga ucguggacgg caacaagaag     3660

uucaugcucg gcuccagcuc cuaccaguac aacgccaagc agcugguccu gagcgacaag     3720

gcgaugcagg uccucuccaa gaacgggaag gucaagagcc aggacgagaa ccagaaccug     3780

aucgacgugu acgacgagau ccuggagaag guggaccagu acuucgagcu cuacgaccag     3840

cgccacuacc gggagucccu gcacaagggc cgcgggaucu ucgugaaccu gccgcucagc     3900

aaccaguacg acggcuccaa gcugacgagc uacgggaagu acgagaccau cgacgccauc     3960

cugaacggcc uccacgccaa cgccaccaug uccaaccugc gguaccuggg caucagcucc     4020

cccuucggga agaugcaggc gagcaacggc cucaccaucg accccgacuc caucaucugc     4080

uaccagagcc ccacggggcu guucgagcgc cagaucaagc uguccgaccu cuga           4134


<210>  2029
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2029
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgaugcagg aggagaagua cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccucg uguaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgacc gcuucgacgu gcggaacacc      540

gacauccaga agcaguacca ggcguuccug gagaucuucg acaccacguu cgagaacaac      600

caccuccucu cccagaacgu cgacguggag gccauccuga ccgacaagau cagcaagucc      660

gccaagaagg accgcauccu ggcccaguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gcggacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucagcgucg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggugacc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcgguacga cgagcaccac gaggaccuga agcaccucaa gcaguucguc     1020

aaggccagcc ugcccgagaa cuaccgcgag guguucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggccuucu acaaguaccu gcucaagcug     1140

cugacgaagc aggaggggag cgaguacuuc cucgagaaga ucaagaacga ggacuuccug     1200

cggaagcagc gcaccuucga caacggcucc aucccccacc aggugcaccu gaccgagcuc     1260

cgcgcgauca uccggcgcca gagcgaguac uacccguucc ugaaggagaa ccaggaccgg     1320

aucgagaaga uccugaccuu ccgcaucccc uacuacgucg gcccccucgc ccgggagaag     1380

uccgacuucg ccuggaugac gcgcaagacc gacgacagca uccggcccug gaacuucgag     1440

gaccuggugg acaaggagaa guccgccgag gccuucaucc accgcaugac caacaacgac     1500

cuguaccugc cggaggagaa ggugcucccc aagcacagcc ugaucuacga gaaguucacc     1560

gucuacaacg agcugacgaa ggugcgguuc cucgcggagg gguucaagga cuuccaguuc     1620

cugaaccgca agcagaagga gaccaucuuc aacucccugu ucaaggagaa gcggaaggug     1680

accgagaagg acaucaucag cuuccucaac aaggucgacg gcuacgaggg gaucgccauc     1740

aagggcaucg agaagcaguu caacgccucc cugagcaccu accacgaccu gaagaagauc     1800

cucgggaagg acuuccugga caacacggac aacgagcuga uccucgagga caucgugcag     1860

acccugaccc uguucgagga ccgcgagaug aucaagaagu gccucgacau cuacaaggac     1920

uucuucaccg agucccagcu gaagaagcug uaccggcgcc acuacacggg cuggggccgg     1980

cucagcgcca agcugaucaa cgggauccgc aacaaggaga accagaagac cauccuggac     2040

uaccucaucg acgacggcuc cgcgaaccgg aacuucaugc agcugaucaa cgacgacgac     2100

cugagcuuca agcccaucau cgacaaggcc cgcaccgggu cccacagcga caaccucaag     2160

gagguggucg gcgagcuggc cggguccccc gccaucaaga agggcauccu gcagagccuc     2220

aagaucgugg acgagcuggu caaggugaug ggcuacgagc cggagcagau cguggucgag     2280

auggcccggg agaaccagac cacggcgaag gggcuguccc gcagccgcca gcggcucacc     2340

acccugcgcg agagccuggc caaccucaag uccaacaucc uggaggagaa gaagcccaag     2400

uacgugaagg accaggugga gaaccaccac cugagcgacg accgccucuu ccuguacuac     2460

cugcagaacg gccgggacau guacaccaag aaggcccucg acaucgacaa ccugucccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagcau cgacaaccgg     2580

guccuggugu ccagcgccaa gaaccgcggg aaguccgacg acgugcccag caucgagauc     2640

gucaaggcgc ggaagauguu cuggaagaac cuccuggacg ccaagcugau gucccagcgc     2700

aaguacgaca accucaccaa ggccgagcgg ggcgggcuga cgagcgacga caaggcccgc     2760

uucauccagc ggcagcuggu ggagacccgc cagaucacca agcacguggc ccggauccuc     2820

gacgagcgcu ucaacaacga ggucgacaac ggcaagaaga ucugcaaggu gaagaucgug     2880

acccugaagu ccaaccuggu cagcaacuuc cggaaggagu ucggcuucua caagauccgc     2940

gaggugaacg acuaccacca cgcgcacgac gccuaccuca acgccguggu cgccaaggcg     3000

auccugacga aguacccgca gcuggagccc gaguucgugu acgggaugua ccggcagaag     3060

aagcucucca agaucgugca cgaggacaag gaggagaagu acagcgaggc cacccgcaag     3120

auguucuucu acuccaaccu gaugaacaug uucaagcggg ucgugcgccu ggccgacggc     3180

agcaucgugg uccggcccgu gaucgagacc gggcgcuaca ugcgcaagac cgccugggac     3240

aagaagaagc acuucgccac ggugcggaag guccucuccu acccccagaa caacaucgug     3300

aagaagaccg agauccagac cggcggcuuc agcaaggaga gcauccuggc gcacgggaac     3360

uccgacaagc ugaucccgcg caagaccaag gacaucuacc ucgaccccaa gaaguacggc     3420

ggguucgaca gccccaucgu ggccuacucc guccuggugg uggccgacau caagaagggc     3480

aaggcccaga agcugaagac ggucaccgag cuccugggga ucaccaucau ggagcggagc     3540

cgcuucgaga agaaccccuc cgccuuccug gagagcaagg gcuaccucaa cauccgggac     3600

gacaagcuga ugauccugcc gaaguacucc cucuucgagc uggagaacgg ccgccggcgc     3660

cugcucgcga gcgccgggga gcugcagaag ggcaacgagc uggcccuccc cacccaguuc     3720

augaaguucc uguaccuggc cucccgguac aacgagagca aggggaagcc cgaggagauc     3780

gagaagaagc aggaguucgu gaaccagcac guguccuacu ucgacgacau ccuccagcug     3840

aucaacgacu ucagcaagcg cgucauccug gccgacgcga accucgagaa gaucaacaag     3900

cuguaccagg acaacaagga gaacaucccg guggacgagc uggccaacaa caucaucaac     3960

cucuucacgu ucaccucccu gggcgccccc gccgcguuca aguucuucga caagaucgug     4020

gaccggaagc gcuacaccag caccaaggag guccugaacu ccacgcucau ccaccagagc     4080

aucaccgggc uguacgagac ccggaucgac cugggcaagc ucggcgagga cuga           4134


<210>  2030
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2030
auggagaaga ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucacggacg acuacaaggu gcccgccaag aagaugaagg ugcucgggaa caccgacaag      120

aaguacauca agaagaaccu gcugggcgcg cuccuguucg acagcgggga gaccgccgag      180

gccacccgcc ugaagcgggc cgcccgccgg cgcuacacgc ggcgcaagaa ccggcuccgc      240

uaccugcagg agaucuucgc gaaggagaug gccaaggucg acgaguccuu cuuccagcgg      300

cuggaggaga gcuuccucac cgacgacgac aagaccuucg acucccaccc gaucuucggc      360

aacaaggccg aggaggacac cuaccaccag gaguucccca cgaucuacca ccuccgcaag      420

caccuggccg acagccccga gaagguggac cugcggcugg uguaccuggc gcucgcccac      480

augaucaagu uccgcggcca cuuccugauc gaggggcagc ugaacgccga gaacaccgac      540

guccagaaga ucuucgccga cuucgugggc guguacgacc ggaccuucga cgacucccac      600

cucagcgaga ucaccgucga cgccgcgucc auccugacgg agaagaucag caagucccgc      660

cggcuggaga accucaucaa gcaguacccc accgagaaga agaacacccu guucgggaac      720

cugguggccc ucgcccuggg ccugcagccg aacuucaaga ccaacuucaa gcucagcgag      780

gacgccaagc ugcaguucuc caaggacacg uacgacgagg accuggagga gcuccugggg      840

aagaucggcg acgacuacgc cgaccuguuc accgcggcca agaaccucua cgacgccauc      900

cugcugagcg gcauccucac cguggacgac aacuccacca aggcgccccu gagcgccucc      960

augaucaagc gcuacgagga gcaccacgag gaccuggaga agcucaagac guucaucaag     1020

gucaacaacu ucgacaagua ccacgagauc uucaaggaca agagcaagaa cggguacgcc     1080

ggcuacaucg agaacggggu gaagcaggac aucuucuaca agcaccugaa guccaucauc     1140

agcgagaaga acggcggcca guacuuccug gacaagaucg agcgggagga cuuccuccgc     1200

aagcagcgga ccuucgacaa cgggagcauc cccuaccaga uccaccucca ggagaugcgc     1260

accauccugc ggcgccaggg cgaguacuac cccuuccuga aggagaacca ggccaagauc     1320

gagaagaucc ugaccuuccg caucccguac uacguggggc cccuggcccg gaagaacucc     1380

cgcuucgccu gggccaagua ccacagcgac gagcccauca cgcccuggaa cuucgacgag     1440

gucguggaca aggagaaguc cgcggagaag uucaucaccc ggaugacccu caacgaccug     1500

uaccugccgg aggagaaggu gcugcccaag cacagcuacg ucuacgagac cuucgccgug     1560

uacaacgagc ucacgaagau caaguacgug aacgagcagg gcaaguccuu cuucuucgac     1620

gccaacauga agcaggagau cuucgaccac gucuucaagg agaaccgcaa ggugaccaag     1680

gccaagcugc ugagcuaccu caacaacgag uucgaggagu uccggaucaa cgaccugauc     1740

gggcuggaca aggacuccaa gagcuucaac gcgucccucg gcaccuacca cgaccugaag     1800

aagauccugg acaagagcuu ccucgacgac aagaccaacg agcagaucau cgaggacauc     1860

gugcugacgc ugacccucuu cgaggaccgc gacaugaucc acgagcggcu gcagaaguac     1920

uccgacaucu ucaccagcca gcagcugaag aagcucgagc gccggcacua caccggcugg     1980

gggcgccugu ccuacaagcu gaucaacggc auccggaaca aggagaacaa caagacgauc     2040

cucgacuucc ugaucgacga cggggacgcc aaccgcaacu ucaugcagcu gaucaacgac     2100

gacagccucu ccuucaagac caccauccag gaggcccagg ucgugggcga cgucgacgac     2160

aucgaggccg uggugcacga ccugcccggg agccccgcca ucaagaaggg cauccugcag     2220

uccgucaaga ucguggacga gcucgugaag gucaugggcc acaacccgca gaacaucgug     2280

aucgagaugg cgcgggagaa ccagaucacc ggguacggcc gcaaccggag caaccagcgc     2340

cugaagcggc ugcaggacuc ccucaaggag uucgggagcg acauccugag caagaagaag     2400

cccuccuacg uggacagcaa ggucgagaac ucccaccucc agaacgaccg ccuguuccug     2460

uacuacaucc agaacggcaa ggacauguac accggggagg agcuggacau cgaccaccuc     2520

agcgacuacg acaucgacca caucaucccc caggccuuca ucaaggacaa cuccaucgac     2580

aaccgggugc ugacgagcuc cgccaagaac cgcggcaaga gcgacgacgu gcccuccauc     2640

gagaucgucc gcaaccgcaa gagcuacugg uacaagcugu acaaguccgg ccucaucagc     2700

aagcggaagu ucgacaaccu gaccaaggcc gagcgcgggg gccugaccga gaccgacaag     2760

gccggguuca ucaagcggca gcucguggag acgcggcaga ucaccaagca cguggcgcag     2820

auccuggacg cccgcuucaa caccaagugc gacgagaacg acaaggucau ccgggacgug     2880

aaggugauca cccugaaguc cagccucguc ucccaguucc gcaaggaguu caaguucuac     2940

aaggugcggg agaucaacga cuaccaccac gcccacgacg ccuaccugaa cgccgugguc     3000

ggcacggcgc ugcucaagaa guacccgaag cuggcccccg aguucgugua cggcgaguac     3060

aagaaguacg acgugcgcaa gcuggucgcc aagagcuccg acaaccacag cgagcucggg     3120

aaggccaccg cgaaguacuu cuucuacagc aaccugauga acuucuucaa gaccgaggug     3180

aaguacgccg acggccgggu guucgagcgc cccgacaucg agaccaacgc cgacggggag     3240

gucgugugga acaagcagcg ggacuucaac aucgugcgca agguccuguc cuacccccag     3300

gugaacaucg ugaagaaggu cgaggugcag acgggcgggu ucagcaagga guccauccuc     3360

ccgaagggcg acagcgacaa gcugaucccc cggaagacca agaagcugca gugggagacc     3420

cagaaguacg gcggguucga cucccccacc guggccuaca gcguccucgu gguggccgac     3480

gucgagaagg gcaagacgcg caagcugaag accgugaagg agcugguggg gaucuccauc     3540

auggagcgga gcuccuucga ggagaacccc gucagcuucc ucgagaagaa gggcuaccac     3600

aacgugcagg aggacaagcu gaucaagcug ccgaaguacu cccucuucga guucgagggc     3660

gggcgccggc gccugcuggc gagcgccacc gagcuccaga agggcaacga ggugguccug     3720

ccccaguaca uggugaaccu gcucuaccac ucccagcacg ugaacaacag ccacaagccc     3780

gagcaccuga acuacgucaa gcagcacaag gacgaguuca aggacaucuu caaccugauc     3840

aucuccaucg cccggaucaa cauccucaag ccgaaggugg uggacaaccu gaucaacgag     3900

uucaccgagu acgggcagga ggacaucagc ucccugagcg aguccuucau caaccuccug     3960

aaguucauca gcuucggcgc ccccggggcc uucaaguucc ugaagcucga cgucaagcag     4020

agcaaccugc gcuacaaguc cacgaccgag gcgcugagcg ccacccucau ccaccagucc     4080

gugaccggcc uguacgagac gcgcaucgac cugagcaagc ucggggagga guga           4134


<210>  2031
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2031
augaagaccu ucgccgagga guacgugaug gacaagagca agcccuacgg caucgggcug       60

gacaucggca ccaacuccgu cggguucgug gccacggacg cggagggcca ccucauccgc      120

cugaagggga agaccgugau cggcgccuac cuguucaacg ccggcaucag cgccgccgag      180

cggcgcgggu uccggaccac ccgccggcgc cucucccggg ucaaguggcg ccugggccug      240

cuccgggaga ucuucgagac gcacuuccag gagagcaugg gggagaacga ggacaacgac      300

uucuuccugc gcuucaagua cuccaacauc agccccaagg acccccaguu cuccaccgcg      360

aagggccugu ucaacgaccg gaccgacaag gaguucuacg accaguaccc caccaucuac      420

caccuccgcc gggcccugau gacggaggac caccaguucg acauccgcga gaucuacauc      480

gccaugcacc acaucgugaa guaccggggg cacuuccuga aggagggccg cgccaaggac      540

uucaaggugg gcgaccuccg gcugcuggac aacuucaaga ugaugaacga gcagaucgag      600

gagaucaacc cccucuggca gcugaagcug ccgaccgacg acgcgagcau caaguccauc      660

accgccaucc uccuggacaa cacccagagc cagaacgacc gccagaaggc cgucacgaag      720

gugauccugg ccacccucgu gaaggccucc gacaaggaca ucaacgcggc ccggaagcgc      780

uucgucgggg agcugagcaa ggccauggug ggccugaaga ccaagcucug ggugcuggcc      840

gacgucuccc agaacgggga cugggagauc aaguacgaga acuacgccga cuucgcggag      900

accaucggca gcggcgaguc cgacacgauc cagagccugu ucaacgagau caacgaccuc      960

uacgggguga ucacccuggc cggcaucauc cccaaggagg ccgaguccuu cagcgacggg     1020

auggugcgga aguacgagca ccaccgcaag aaccuggagc uccugaaggu cuacugcgcc     1080

gagcaguccg acggcaagcg cgggcggcag auccgccaga ccuacgacaa guacaucgac     1140

ggcguggaca gcaagcaguu cacccaggag gacuucuaca aggcccugag caaguucacg     1200

gcgaaggacg aggccaccuc cgagaacgcc aagcucaucg cccaggagau cgccgugggc     1260

accuucaugc ccaagcugcg gaccaaggcg aacgggacga ucccccacca gcugcaccag     1320

aaggagcucg acgccaucau cgagaaccag aagaaguacu acccguggcu gggcgagguc     1380

aaccccgugg agagccaccg ccgggcccug cccuacaagc ucgacgagcu gguguccuuc     1440

cgcauccccu acuacgucgg gccgauggug acccccacca agggcgaccc cgagaagucc     1500

aaguucgccu ggauggugcg gaaggagccc gggaccauca cgccguggaa cuucgaccag     1560

aaggucgacc gcagcgcgag cggcgaggcc uucauccagc ggaugaagac caccgacacc     1620

uuccugaucg gcgaggacgu gcucccccag cagucccugc uguaccagaa guucgagguc     1680

cucaacgagc ugaacaagau caugaucaac gggaagccca ucugccgcga gcagaagcag     1740

cggcuguuca agcagcuguu caugcaguac aagacgguga ccgugaagaa gguccagcag     1800

aaccucaucg ccaacggcga ggagagcgag aacgugccca ucaccgggcu guccgacccg     1860

cugcgcuuca acagcuccuu cagcaccuac aucgacuaca aggacauccu cggcacggcc     1920

gccgugaacg acaacgcgaa gcaguccgac aucgagcaga ucaucgccug gagcaccauc     1980

uucgaggacg ccgccaucuu ccgggagaag cugaacgaca ucaccuggcu gaacgacgac     2040

cagcgcaaca agcucuccca caagcgguac cgcggguggg gccggcacag ccgcaagcug     2100

cuggccggcc uccgggacgg ggagggccag accaucaucg agcgccugug gaacaccaac     2160

gacaacuuca ugcagaucca gaacgacucc gagaucgcgc gccagaucac ggaggccaac     2220

agcuccaaga uggccaccgc cgaggggacc gacgagauca ucgacggcuu cuacaccagc     2280

cccgagaaca agaaggcccu gcgggagguc augaaggugg ugaaggacau ccagcgcgcg     2340

caccacggcc aggcccccgc cugggucuac aucgagagcc cccgggagac gccgcgcccc     2400

gggcagcgga ccgugucccg cgagcagcag cucaccgacc uguacgaggg cgccgcgaag     2460

gagaucgugg acgacgccgu ccugaacgag cucaaggaca aggucaagag caaggagaac     2520

uucaccgaca agcuggugcu guacuuccuc cagaacgggc acgacaucua cgccaacgac     2580

uccaucaaca ucgacaaccu gaacgccuac gacaucgacc acgugcugcc ccagagccuc     2640

aucaaggacg acucccugga caaccgggug cugacgaccc acgagcgcaa ccucaagaag     2700

uccaaccggu ucgccaccga gcuguucgcg gaccagcgca agaaguggga gaaguggcac     2760

cggcugggcc ucaucagcag ccgcaagcug aagcaccuga ccaugcagcc caacuccgug     2820

gagaaguucg cccacggguu caucgcccgg cagcucacgg agacccgcca gaucauccac     2880

cugaccgcca acguccugag caaccucuac caggagaacg acaccaagau cgugaugauc     2940

aaggccggcc ugaacuccga guuccggcgc acguucgacu ucccgaagaa ccggagcgug     3000

aacgacuacc accacgcguu cgacgccuuc cugaccgcca agaucggccg cuaccuccug     3060

gcccgguacc ccaagcugga gcccuucuuc gucuacggga acuucgugaa gaacccgaag     3120

gccaugaagc gccucuccag cuucgacuuc aucgcgcagc uggccgccaa gaccgacgac     3180

accucccaca ucgaccagcg gagccugaag cagguccccg uggugaacga ggagacgggc     3240

gagaucgugu gggacaagga caucgagcuc gccgagcugg acaagaccua caacuacaag     3300

accaugcugg ucaagcgcgc gcagaccgag aacaacgccc agauguucaa gcagacggug     3360

uucaaggccc gcgacaacca gaacaagacc cucauccccg ugaagaacgg gcuguccacc     3420

gacgucuacg gcgggcacag ccagcaggcc aucagcuacc ugugcaucgu gugggugggc     3480

cagaagaaga aguaccgggu ccucggcauc uccaccgccc acgcggggau ccugaacaac     3540

uucgagaaga acuacggccg cuucgaggcc aagaagaagc ugcaggagau cgugagcaac     3600

acgcucgaca acgccgaccg gaacgacuuc aagaucgugg cccccaaggu ccuguucgag     3660

caggucgugg aggacgacaa caugaaguuc gggcugggcu ccgccagcga cuaccgcaac     3720

gugcagcagc uguuccucuc ccggaagaac cagcugcucc uggcgaacau gaugaccgac     3780

cagauccacg accaggaccu ggugcaccuc uucgacgaga ucguggggca gaugaacgcc     3840

cacuucccga ucuucgaccg cggcggcuac cggagcuccc ugacccagag ccgcgacaag     3900

uuccugaagc uccccuucaa gaagaacgag gaccugauca ccaagcagga ggucauccgg     3960

cgcauccugg acgggcucca cgccaacgcc aaccggaagg accugaagau caucggcucc     4020

aagggggacu ucggccgccu ggggacgaag aagaucuacc ucagcaagga cgccaagcug     4080

aucuacaccu ccccgaccug ccuguucacc cggacggugc cccucagcuc ccuguga        4137


<210>  2032
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2032
augaccaacg agaaguacag caucggccug gacaucggga ccuccagcau cggcuucgcc       60

guggucaacg acaacaaccg cgugauccgg gugaagggga agaacgccau cggcguccgc      120

cucuucgacg aggggaaggc ggccgccgac cggcgcuccu uccggacgac ccgccggcgc      180

cugagccggc gccgguggcg ccugaagcuc cugcgggaga ucuucgacgc cuacaucacc      240

cccguggacg aggccuucuu cauccgccug aaggagucca accucagccc gaaggacucc      300

aagaagcagu acagcggcga cauccuguuc aacgaccggu ccgacaagga cuucuacgag      360

aaguacccca ccaucuacca ccuccgcaac gcgcugauga cggagcaccg gaaguucgac      420

gugcgcgaga ucuaccuggc cauccaccac aucaugaagu uccggggcca cuuccugaac      480

gccacccccg ccaacaacuu caaggucggg cgccucaacc uggaggagaa guucgaggag      540

cugaacgaca ucuaccagcg gguguucccc gacgagagca ucgaguuccg caccgacaac      600

cucgagcaga ucaaggaggu gcugcuggac aacaagcgcu cccgggcgga ccgccagcgg      660

acccucguca gcgacaucua ccaguccagc gaggacaagg acaucgagaa gcgcaacaag      720

gccguggcca cggagauccu gaaggccucc cugggcaaca aggccaagcu caacgugauc      780

accaacgucg agguggacaa ggaggcggcc aaggagugga gcaucaccuu cgacuccgag      840

agcaucgacg acgaccuggc caagaucgag gggcagauga ccgacgacgg ccacgagauc      900

aucgaggugc ugcggucccu cuacagcggg aucacgcuga gcgccaucgu cccggagaac      960

cacacccugu cccagagcau gguggccaag uacgaccucc acaaggacca ccugaagcug     1020

uucaagaagc ucaucaacgg caugaccgac accaagaagg cgaagaaccu gcgcgccgcc     1080

uacgacggcu acaucgacgg ggugaagggc aagguccugc cccaggagga cuucuacaag     1140

cagguccagg ugaaccucga cgacuccgcc gaggccaacg agauccagac guacaucgac     1200

caggacaucu ucaugcccaa gcagcggacc aaggcgaacg ggagcauccc ccaccagcug     1260

cagcagcagg agcuggacca gaucaucgag aaccagaagg ccuacuaccc guggcucgcc     1320

gagcugaacc ccaaccccga caagaagcgc cagcagcugg ccaaguacaa gcucgacgag     1380

cuggugaccu uccgggugcc cuacuacgug ggcccgauga ucaccgccaa ggaccagaag     1440

aaccaguccg gcgcggaguu cgccuggaug auccgcaagg agcccgggaa caucacgccc     1500

uggaacuucg accagaaggu cgaccggaug gccaccgcca accaguucau caagcgcaug     1560

accaccacgg acaccuaccu gcucggcgag gacgugcugc ccgcgcagag ccugcucuac     1620

cagaaguucg agguccugaa cgagcugaac aagauccgga ucgaccacaa gccgaucucc     1680

aucgagcaga agcagcagau cuucaacgac cucuucaagc aguucaagaa cgugaccauc     1740

aagcaccucc aggacuaccu ggugagccag gggcaguacu ccaagcgccc ccugaucgag     1800

ggccuggccg acgagaagcg guucaacagc ucccugagca ccuacuccga ccugugcggg     1860

aucuucggcg ccaagcucgu cgaggagaac gaccgccagg aggaccugga gaagaucauc     1920

gaguggagca cgaucuucga ggacaagaag aucuaccggg ccaagcugaa cgaccucacc     1980

uggcugaccg acgaccagaa ggagaagcug gccaccaagc gcuaccaggg cugggggcgg     2040

cucucccgca agcugcuggu gggccucaag aacagcgagc accgcaacau cauggacauc     2100

cuguggauca cgaacgagaa cuucaugcag auccaggcgg agcccgacuu cgccaagcug     2160

gugaccgacg ccaacaaggg gaugcucgag aagaccgacu cccaggacgu caucaacgac     2220

cuguacacca gcccccagaa caagaaggcc auccggcaga uccugcucgu ggugcacgac     2280

auccagaacg ccaugcacgg ccaggcgccg gccaagaucc acgucgaguu cgcccgcggg     2340

gaggagcgga acccccgccg guccgugcag cgccagcgcc agguggaggc cgccuacgag     2400

aaggucagca acgagcuggu gagcgcgaag gugcggcagg aguucaagga ggccaucaac     2460

aacaagcggg acuucaagga ccgccugcuc cuguacuuca ugcagggcgg caucgacauc     2520

uacacgggga agcagcugaa caucgaccag cucuccagcu accagaucga ccacauccug     2580

ccccaggccu ucgucaagga cgacucccug accaaccggg ugcucaccaa cgagaaccag     2640

gugaaggccg acagcguccc caucgacauc uucggcaaga agaugcuguc cguguggggg     2700

cgcaugaagg accagggccu gaucagcaag gggaaguacc ggaaccucac caugaacccg     2760

gagaacaucu ccgcgcacac cgagaacggc uucaucaacc gccagcuggu ggagacgcgg     2820

caggucauca agcuggccgu gaacauccuc gccgacgagu acggcgacag cacccagauc     2880

aucuccguga aggccgaccu gagccaccag augcgcgagg acuucgagcu gcucaagaac     2940

cgggacguca acgacuacca ccacgccuuc gacgcguacc uggccgccuu caucgggaac     3000

uaccugcuca agcgcuaccc caagcuggag uccuacuucg uguacggcga cuucaagaag     3060

uucacccaga aggagaccaa gaugcggcgc uucaacuuca ucuacgaccu gaagcacugc     3120

gaccaggugg ucaacaagga gacgggggag auccucugga ccaaggacga ggacaucaag     3180

uacauccggc accuguucgc cuacaagaag auccugguga gccacgaggu gcgcgagaag     3240

cgcggcgccc ucuacaacca gaccaucuac aaggcgaagg acgacaaggg cuccgggcag     3300

ggcagcaaga agcugauccg gaucaaggac gacaaggaga ccaagaucua cgggggcuac     3360

uccggcaaga gccuggccua caugacgauc guccagauca ccaagaagaa caaggugucc     3420

uaccgcguga ucgggauccc cacccucgcc cuggcccggc ugaacaagcu cgagaacgac     3480

agcaccgaga acaacggcga gcuguacaag aucaucaagc cccaguucac gcacuacaag     3540

gucgacaaga agaacgggga gaucaucgag accaccgacg acuucaagau cguggugagc     3600

aagguccgcu uccagcagcu gaucgacgac gccggccagu ucuucaugcu cgcguccgac     3660

accuacaaga acaacgccca gcagcuggug aucagcaaca acgcccugaa ggccaucaac     3720

aacacgaaca ucaccgacug cccgcgggac gaccucgagc gccuggacaa ccugcggcuc     3780

gacuccgcgu ucgacgagau cgugaagaag auggacaagu acuucagcgc cuacgacgcc     3840

aacaacuucc gcgagaagau ccggaacucc aaccugaucu ucuaccagcu gcccgucgag     3900

gaccaguggg agaacaacaa gaucaccgag cucgggaagc gcaccgugcu gacgcggauc     3960

cugcagggcc uccacgccaa cgccaccacc accgacauga gcaucuucaa gaucaagacg     4020

cccuucgggc agcugcgcca gcgguccggc aucagccugu ccgagaacgc gcagcucauc     4080

uaccagagcc cgaccggccu guucgagcgc cgggugcagc ugaacaagau caaguga        4137


<210>  2033
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2033
augaagaaca aggacuacag caucgggcug gacaucggga ccuccagcau cggcuucgcc       60

guggucgacg agaacaacca cgugaugcgc gugaagggga agaacgccau cggcguccgg      120

cuguucgacg aggggaagcc cgcggccgac cgccgggccu uccgcaccac gcggcgccgg      180

cugucccgcc ggcgcuggcg gcugaagcuc cugcgcgaga ucuucgacag cuacaucacc      240

ccgguggacg aggccuucuu cauccggcug aaggagucca accucagccc caaggacucc      300

aagaagcagu acagcggcga cauccuguuc aacgaccgcu ccgacaagga cuucuacacc      360

aaguacccca ccaucuacca ccuccggaac gcccucauga cggagcacca gcgcuucgac      420

auccgggaga ucuaccuggc gauccaccac aucgugaagu uccgcggcca cuuccugaac      480

gccacccccg ccaacaacuu ccaggucggg aagcucgacc uggaccagaa guucgaggac      540

cugaacgaca ucuaccagcg gguguucccg gacgacagca ucgaguuccg caucgacaac      600

cucgagcaga ucaaggccgu gcugcuggac uccaagcgga gccgcuccga ccgccagcgg      660

agccucgucg acgacgugua ccagaccucc gaggacaagg agguggagaa gcagaacaag      720

gcggucgcca ccgaggugcu gaaggccauc cugggcaaca aggccaagcu caacaucauc      780

gccaacgugg aggucgacaa ggaggcggcc aaggagugga cgaucaccuu cgacagcgag      840

uccaucgacg acgaccuggc caagaucgac gggcagauga ccgacgacgg ccgcgccauc      900

guggagaucc ugcggagccu cuacuccggg aucacccuga gcgccaucgu gcccgagaac      960

cacacgcuga gccaggcgau ggucgccaag uacgaccucc acaagaagca ccugacccug     1020

cucaagaagc ugaucaacuc caugaccgac accaagaagg ccaagaaccu gcgcgccgcc     1080

uacgacggcu acguggacgg cgugaagggg aagguccuca cgcaggacga guucuacaag     1140

cagauccaga gcaaccugga cgaguccgug gaggcgagcg agauccagac cuacaucgac     1200

caggacaucu ucaugcccaa gcagcggacc aaggccaacg gcuccauccc ccaccagcug     1260

cagcagcagg agcucgacca gaucaucgag aaccagaagg aguacuaccc guggcuggcc     1320

gagcugaacc ccaaccccga caagaagcgc cagcagcucg ccaaguacaa gcuggacgag     1380

cuggugaccu uccggguccc cuacuacgug gggccgauga ucacggccga ggcgcagaag     1440

aagcagagcg gcgccgaguu cgccuggaug auccgcaagg aguccggcaa caucaccccc     1500

uggaacuucg accagaaggu cgaccggaug gccaccgcga accaguucau caagcgcaug     1560

accacgaccg acaccuaccu ccugggggag gacgugcugc ccagccaguc ccuccuguac     1620

cagcgguucg aggugcugaa cgagcucaac aagauccgca ucgaccggaa gcccaucagc     1680

gucgagcuga agcagcagau cuucaacgac cuguucaaga aguucaagaa cgugaccauc     1740

aagcaccucc aggacuaccu cguggcccag ggccaguacu ccaagcgccc gcugaucgag     1800

gggcuggccg acgagaagcg guucaacagc ggccucucca cguacaacga cuucugcggg     1860

aucuucggcg ccaagcuggu cgacgagaac gaccgccagg ccgaccugga gaagaucauc     1920

gaguggagca ccaucuucga ggacaagaag aucuaccggg cgaagcucaa cgaccuggcc     1980

uggcugaccg acgagcagaa ggacaagcuc gccaccaagc gcuaccaggg cugggggcgg     2040

cuguccagca agcugcucau cggccugcag aacuccgagc accgcaacau cauggaccug     2100

cucuggaucg ggaacgagaa cuucaugcag auccaggccg acagcgacuu cgccaagcug     2160

aucaaggacg cgaaccagaa cgcccugcag aacaacgacu cccaggacgu gaucaacgac     2220

cucuacacga gcccccagaa caagaaggcc auccgccaga uccugcuggu gguccacgac     2280

auccagaacg ccaugcacgg ccaggccccc accaagaucc acguggaguu cgcgaagggg     2340

gaggagcgga acccccgccg gagcgugcag cgccagcggc aggucgaggc cgccuacgag     2400

aaggccucca acgagcucgu gggcgcgaac gugcgcgagg agcugaagga cgccaucacc     2460

aacaagcggg acuucaagga ccgccuguuc cucuacuuca ugcagggcgg ggucgacaug     2520

uacagcggcg agccgaucaa caucgacaac cugucccggu acgacaucga ccacauccug     2580

ccccaggccu ucgugaagga cgacagccuc aacaaccgcg ugcugaccga cgccgagaag     2640

aaccggcgca aguccgacgc cguccccaac gagcuguucg gggcgaagau gcgggugacg     2700

ugggagcgca ugaaggagca gggccucauc agcaagggga aguaccggaa ccugaccaug     2760

aaccccgaga acaucaacaa guacaccgag aacggcuuca ucaaccgcca gcugguggag     2820

acccggcagg ucaucaagcu cgccgugaac auccuggccg acgaguacaa ggagaacacc     2880

cagaucaucu ccgugaaggc cgaccugagc caccagaugc gcgaggacuu cgagcuccug     2940

aagaaccggg acgucaacga cuaccaccac gccuucgacg cguaccuggc cgccuucaug     3000

ggcaacuacc uccugaagcg cuacccgaag cugcgguccu acuucgugua cggggacuuc     3060

aagaaguuca cgcagaagga gaucaagcuc cgccgcuuca acuucaucua cgaccugaag     3120

cacgaggacc aggugaucga caaggagacc ggcgagaucc uguggaacaa gaacgaggac     3180

aucgccuaca uccggcaccu cuucgccuac aagaagaucc ugaucagcca cgaggucucc     3240

gagcgccgcg gggcgcugua caaccagacc guguacaagg ccagcgacga caaggccucc     3300

ggccagggca gcaagaagcu cauccccauc aaguccgaca agcccaccga gaucuacggg     3360

ggcuacacgg ggcggacccu ggccuacaug accaucguga aggucaccaa gggcaagaag     3420

aucagcuaca aggugguggg gguccccacg gcggugcugg ccaagauggc cggcgugcgg     3480

cagggcuccc aggaggagca ggacaagcuc uacggggucc ugaagccgca guucacccac     3540

cagaaggaga acaagaagac cggcgagauc accgagacgg uggacaaguu cgacaucauc     3600

gugcccaagg ucaaguacca gcagcuggug aacgacaacg ggcagcuguu caugaucggc     3660

agcgacaccc ugcccuacaa cgccgagcag cuggugcuca gcaaggaguc cguccagacc     3720

aucaacaacc cggggaucaa ggacgagggc agcgacgagg aguccgagaa caagaagcug     3780

gacaacgugu acgacgagau cgugcugugc augaacaagu acuucccccu cuacgacaac     3840

cgcggcuucc ggaacagcau caccaacggg aagaacaagu uccugaagcu gcccaucaag     3900

aacaucuaca agaacggcaa gcucaucaac gucgggaagc gcgaggugau caaccggauc     3960

cugcagggcc ugcacgccaa cgcguccgug acggaccuca gcaaccuggg gaucaagucc     4020

cccuucggcu uccugcagaa gggcgccggg cucagccugu ccggcgaggc ccagcugauc     4080

caccagagcg ccaccggccu cuucgagcac cgcguccagc ugaacaagau caaguga        4137


<210>  2034
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2034
augagcaaca agcccuacuc caucggccug gacaucggga ccaacagcgu gggcugggcc       60

gucaucaccg acgacuacaa ggugccgucc aagaagauga aggugcucgg gaacacggac      120

aagcacuuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcg      180

gagggccgcc ggcugaagcg caccgcccgg cgccgguaca cgcgccggaa gaaccgccug      240

cgguaccucc aggagaucuu cagcgaggag aucuccaagg ucgacagcuc cuucuuccac      300

cgccuggacg acagcuuccu ggugcccgag gacaagcggg gcuccaagua ccccaucuuc      360

gccacccucg ccgaggagaa ggaguaccac aagaacuucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacagcaa ggagaaggcg gaccuccggc ugaucuaccu ggcccucgcc      480

cacaugauga aguaccgcgg gcacuuccug uacgaggagu ccuucgacau caagaacaac      540

gacauccaga agaucuucaa cgaguucauc agcaucuacg acaacaccuu cgagggcucc      600

agccuguccg agcagaacgu gcaggucgag gccaucuuca cggacaagau cagcaagucc      660

gcgaagcggg agcgcgugcu caagcuguuc ccggacgaga aguccaccgg gcuguucagc      720

gaguuccuca agcugaucgu gggcaaccag gccgaguuca agaagcacuu cgaccuggag      780

gagaaggcgc cccuccaguu cagcaaggac accuacgacg aggaccugga gaaccugcuc      840

gggcagaucg gcgacggcuu cgccgaccug uuccuggucg ccaagaagcu cuacgacgcc      900

auccugcuga gcgggauccu caccgugacg gaccccucca ccaaggcgcc ccugagcgcc      960

uccaugaucg agcgguacga gaaccaccag aaggaccugg ccagccucaa gcaguucauc     1020

aagaacaacc ugccggccga gaagugcgug gaggucuucu ccgaccagag caaggacggc     1080

uacgccgggu acaucgacgg caagaccacc caggaggccu ucuacaagua caucaagaac     1140

cugcucucca agcuggaggg cgccuacuac uuccuggaca agaucgagcg cgaggacuuc     1200

cuccggaagc agcgcacguu cgacaacggg agcauccccc accagaucca ccuccaggag     1260

augaacgcca uccugcggcg ccagggcgag cacuaccccu uccugaagga gaaccgcgag     1320

aagaucgaga agauccugac cuuccggauc cccuaccacg uggggccgcu ggcccgcggc     1380

aaccgggacu ucgcguggcu cacccgcaac uccgacgagg ccauccggcc cuggaacuuc     1440

gaggagaucg uggaccaggc cagcuccgcc gaggacuuca ucaacaagau gaccaacuac     1500

gaccuguacc ugcccgagga gaagguccug cccaagcaca gccuccugua cgagacguuc     1560

gccguguaca acgagcugac caaggugaag uucaucgcgg aggggcucac ccgcuaccag     1620

uuccuggaca agaagcagaa gaaggacauc uucuacaccu ucuucaaggc cgaggacaag     1680

cggaagguca cggagaagga caucauccac uaccugcaca acguggacgg cuacgacggc     1740

aucgagcuca aggggaucga gaagcaguuc aacgccuccc ugagcaccua ccacgaccug     1800

cucaagauca ucaaggacaa ggaguucaug gacgacccga agaacgagga gauccuggag     1860

aacaucgugc acacccugac caucuucgag gaccgcgaga ugaucaagca gcggcucgcc     1920

caguacgacu cccuguucga cgagaagguc aucaaggcgc ugacgcgccg gcacuacacc     1980

ggcuggggga agcucagcgc caagcugauc aacggcaucu gcgacaagca gaccgggaag     2040

accauccugg acuaccucau cgacgacggc aagaucaacc gcaacuucau gcagcugauc     2100

aacgacgacg gccuguccuu caaggagauc auccagaagg cccagguggu ggggaagacg     2160

uacgacguca agcaggucgu gcaggagcuc cccggcagcc ccgccaucaa gaaggggauc     2220

cugcaguccg ugaagcuggu ggacgagcuc gucaagguga ugggccacgc ccccgagagc     2280

aucgugaucg agauggcgcg ggagaaccag accaccgcca aggggaagaa gaacagccag     2340

cagcgcuaca agcggaucga ggacgccauc aagaaccugg ccccgggccu ggacuccacc     2400

auccucaagg agcaccccac ggacaacauc cagcugcaga acgaccgccu guuccucuac     2460

uaccugcaga acggccggga cauguacacc gggaagcccc uggagaucaa ccagcucagc     2520

aacuacgaca ucgaccacau caucccccag gccuucauca aggacgacuc ccuggacaac     2580

cgcguccuga ccagcuccaa ggagaaccgg ggcaagagcg acaacgugcc gucccucgag     2640

guggucgaga agaugaaggc guucuggcag cagcugcugg acagcaagcu caucuccgag     2700

cgcaaguuca acaaccugac caaggccgag cgcgggggcc uggacgagcu cgacaaggug     2760

ggcuucauca agcggcagcu gguggagacc cgccagauca cgaagcacgu cgcccagauc     2820

cuggacgccc gguucaacaa ggaggugacc gagaagaaca agaagaaccg caacgugaag     2880

aucaucaccc ucaagagcaa cauggucucc aacuuccgga aggaguucgg gcuguacaag     2940

gugcgcgaga ucaacgacua ccaccacgcc cacgacgcgu accugaacgc cguggucgcc     3000

aaggccaucc ucaagaagua ccccaagcug gagcccgagu ucguguacgg cgacuaccag     3060

aaguacgacc ugaagcggua caucagcaag uccaaggacc ccaaggaggu ggagaaggcg     3120

accgagaagu acuucuucua cagcaaccuc cugaacuucu ucaaggagga gguccacuac     3180

gccgacggga cgaucgugaa gcgcgagaac aucgaguacu ccaaggacac cggcgagauc     3240

gccuggaaca aggagaagga cuucgccacc aucaagaagg ugcugagcuu cccgcagguc     3300

aacaucguga agaagaccga ggagcagacg guggggcaga acggcggccu cuucgacaac     3360

aacaucgucu ccaaggagaa ggugguggac gccagcaagc ugauccccau caagagcggg     3420

cugucccccg agaaguacgg cggguacgcg cggccgacca ucgccuacag cguccucgug     3480

accuacaagc agaacaacaa gaucaagaac accauggugg gcauccccgu ccugacgaag     3540

cuggaguucg agcgcgaccc caaccucuac cugcggaacc uggggaucaa caacgugucc     3600

cacgugauca agcucgucaa gcacaccgug cuggaguuca agcacaagua cggcaccuac     3660

cgccgguuca ucgugagcaa ccaggagcug aagcgcgcca accagaucuu cgucaccacg     3720

aagcagauga agcucaucuc ccaguacacc acccuggaca agaacgagca cgacagccug     3780

cucaagaaca accagcagcu gauccagucc aucuggaacc aguuccugga guucaugcuc     3840

gccaccgacc uggugaacaa gaagcaguac gacgagcuga uccucacgaa caaccagacc     3900

aucgaggagu gccggaacau caugaaccgc gugaucacca cccugagcuu cacgaacauc     3960

aacgccggcc agaagaagau caucuucgac gacacccugu cccuccccau caagcggugg     4020

caguucaccg acagcgacaa gauggucaag aagaucaucg aguccacccu gauccaccag     4080

agcaucacgg ggcuguacga gacccgcauc gaccucaaca agcugggcga ggacuga        4137


<210>  2035
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2035
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cugcuguucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgaugcagg aggagaagua cuaccacgag aaguucccca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccuca ucuaccuggc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgacc gcuucgacgu gcggaacacc      540

gacauccaga agcaguacca ggcguuccug gagaucuucg acaccacguu cgagaacaac      600

gaccugcucu cccagaacgu ggacgucgag gccauccuga ccgacaagau cagcaagucc      660

gccaagaagg accgcauccu ggcccgguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gcggacuuca agaagcacuu caaccuggag      780

gacaagaccc cgcuccaguu cgccaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucagcgccg ccaagaagcu guacgacucc      900

gugcuccuga gcggcauccu gacggucauc gaccucucca ccaaggcgcc ccugagcgcc      960

uccaugaucc agcgcuacga cgagcaccac gaggaccuga agcaccucaa gcaguucgug     1020

aaggccagcc ugcccgagaa cuaccgggag guguucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccagccag gaggcguucu acaaguaccu gcucaagcug     1140

cugaccaagc aggagggguc cgaguaccuc cuggagaaga ucaagaacga ggacuuccug     1200

cgcaagcagc gcacguucga caacggcagc aucccccacc agguccaccu caccgagcug     1260

cgggccauca uccgccggca guccgaguac uacccguucc ugaaggagaa ccucgaccgc     1320

aucgagaaga uccugaccuu ccggaucccc uacuacgugg gcccccuggc ccgcgagaag     1380

uccgacuucg ccuggaugac ccggaagacg gacgacagca uccgccccug gaacuucgag     1440

gaccuggugg acaaggagaa guccgccgag gcguucaucc accggaugac caacaacgac     1500

cucuaccugc cggaggagaa gguccugccc aagcacagcc ucaucuacga gaaguucacc     1560

guguacaacg agcugaccaa ggugcgcuuc cuggccgagg gguucaagga cuuccaguuc     1620

cucaaccgga agcagaagga gacgaucuuc aacagccugu ucaaggagaa gcgcaagguc     1680

accgagaagg acaucaucuc cuuccugaac aagguggacg gcuacgaggg gaucgccauc     1740

aagggcaucg agaagcaguu caacgccagc cucuccaccu accacgaccu gaagaagauc     1800

cuggggaagg acuuccucga caacaccgac aacgagcuga uccuggagga caucauccag     1860

acgcucaccc uguucgagga ccgggagaug aucaagaagc gccuggacau cuacaaggac     1920

uucuucaccg agagccagcu caagaagcug uaccggcgcc acuacaccgg cuggggccgg     1980

cuguccgcga agcucaucaa cgggauccgc aacaaggaga accagaagac gauccuggac     2040

uaccugaucg acgacggcag cgccaaccgg aacuucaugc agcucaucaa ggacgccggg     2100

cuguccuuca agcccaucau cgacaaggcc cgcaccggca gccacuccga caaccugaag     2160

gagguggucg gggagcucgc cggcagcccc gcgaucaaga agggcauccu gcagucccug     2220

aagaucgugg acgagcucgu gaaggucaug ggguacgagc cggagcagau cguggucgag     2280

auggcccgcg agaaccagac caccgccaag ggccugagcc ggagccgcca gcggcugacg     2340

acccuccgcg agucccuggg gaaccucaag agcaacaucc ucgaggagaa gaagcccaag     2400

uacgugaagg accaggugga gaaccaccac cuguccgacg accggcuguu ccucuacuac     2460

cugcagaacg gcaaggacau guacaccgac gacgagcugg acaucgacaa ccucagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacuccau cgacaaccgc     2580

guccugguga gcuccgccaa gaaccggggc aagagcgacg acgugcccuc caucgagauc     2640

gucaaggcgc gcaagauguu cuggaagaac cugcucgacg ccaagcugau gagccagcgg     2700

aaguacgaca accugaccaa ggccgagcgc gggggccuca ccuccgacga caaggcccgg     2760

uucauccagc gccagcuggu ggagacgcgg cagaucacca agcacguggc ccgcauccug     2820

gacgagcggu ucaacaacga gcucgacagc aaggggcgcc ggauccgcaa ggucaagauc     2880

gugacccuga aguccaaccu ggugagcaac uuccggaagg aguucggcuu cuacaagauc     2940

cgcgagguca acaacuacca ccacgcgcac gacgccuacc ucaacgccgu gguggccaag     3000

gcgauccuga ccaaguaccc gcagcuggag cccgaguucg ucuacgggcu cuaccggcgc     3060

aagaagcugu ccaagaucgu gcgcgaggac aaggaggaga aguacagcga ggccacgcgg     3120

aagauguucu ucuacuccaa ccugaugaac auguucaagc gcgugguccg gcucgccgac     3180

ggcagcaucg uggugcgccc cgucaucgag accggccggu acauggggaa gaccgccugg     3240

gacaagaaga agcacuucgc caccgugcgc aaggugcuga gcuaccccca gaacaacauc     3300

gucaagaaga cggagaucca gaccggcggg uucuccaagg agagcauccu ggcgcacggc     3360

aacuccgaca agcucauccc gcggaagacc aaggacaucu accuggaccc caagaaguac     3420

gggggcuucg acagccccau cguggccuac uccgugcugg ucguggccga caucaagaag     3480

ggcaaggccc agaagcucaa gaccgugacg gagcugcugg ggaucaccau cauggagcgc     3540

agccgguucg agaagaacac cuccgccuuc cucgagagca agggcuaccu gaacauccgc     3600

gacgacaagc ugaugauccu ccccaaguac ucccuguucg agcuggagaa cgggcggcgc     3660

cggcuccugg cgagcgccgg cgagcugcag aaggggaacg agcucgcccu gccgacccag     3720

uucaugaagu uccuguaccu cgccucccgc uacaacgaga gcaagggcaa gcccgaggag     3780

aucgagaaga agcaggaguu cgucaaccag cacguguccu acuucgacga cauccugcag     3840

cugaucaacg acuucagcaa ccgggugauc cucgccgacg cgaaccugga gaagaucaac     3900

aagcuguacc aggacaacaa ggagaacauc cccgucgacg agcucgccaa caacaucauc     3960

aaccuguuca cguucaccuc ccugggcgcc ccggccgcgu ucaaguucuu cgacaagauc     4020

guggaccgca agcgguacac cagcaccaag gaggugcuca acuccacgcu gauccaccag     4080

agcaucaccg ggcuguacga gacccgcauc gaccucggca agcuggggga gggcuga        4137


<210>  2036
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2036
augaccaacg agaaguacag caucggccug gacaucggga ccuccagcau cggcuucgcc       60

guggucaacg acaacaaccg cgugauccgg gugaagggga agaacgccau cggcguccgc      120

cucuucgacg aggggaaggc ggccgccgac cggcgcuccu uccggacgac ccgccggcgc      180

cugagccggc gccgguggcg ccugaagcuc cugcgggaga ucuucgacgc cuacaucacc      240

cccguggacg aggccuucuu cauccgccug aaggagucca accucagccc gaaggacucc      300

aagaagcagu acagcggcga cauccuguuc aacgaccggu ccgacaagga cuucuacgag      360

aaguacccca ccaucuacca ccuccgcaac gcgcugauga cggagcaccg gaaguucgac      420

gugcgcgaga ucuaccuggc cauccaccac aucaugaagu uccggggcca cuuccugaac      480

gccacccccg ccaacaacuu caaggucggg cgccucaacc uggaggagaa guucgaggag      540

cugaacgaca ucuaccagcg gguguucccc gacgagagca ucgaguuccg caccgacaac      600

cucgagcaga ucaaggaggu gcugcuggac aacaagcgcu cccgggcgga ccgccagcgg      660

acccucguca gcgacaucua ccaguccagc gaggacaagg acaucgagaa gcgcaacaag      720

gccguggcca cggagauccu gaaggccucc cugggcaaca aggccaagcu caacgugauc      780

accaacgucg agguggacaa ggaggcggcc aaggagugga gcaucaccuu cgacuccgag      840

agcaucgacg acgaccuggc caagaucgag gggcagauga ccgacgacgg ccacgagauc      900

aucgaggugc ugcggucccu cuacagcggg aucacgcuga gcgccaucgu cccggagaac      960

cacacccugu cccagagcau gguggccaag uacgaccucc acaaggacca ccugaagcug     1020

uucaagaagc ucaucaacgg caugaccgac accaagaagg cgaagaaccu gcgcgccgcc     1080

uacgacggcu acaucgacgg ggugaagggc aagguccugc cccaggagga cuucuacaag     1140

cagguccagg ugaaccucga cgacuccgcc gaggccaacg agauccagac guacaucgac     1200

caggacaucu ucaugcccaa gcagcggacc aaggcgaacg ggagcauccc ccaccagcug     1260

cagcagcagg agcuggacca gaucaucgag aaccagaagg ccuacuaccc guggcucgcc     1320

gagcugaacc ccaaccccga caagaagcgc cagcagcugg ccaaguacaa gcucgacgag     1380

cuggugaccu uccgggugcc cuacuacgug ggcccgauga ucaccgccaa ggaccagaag     1440

aaccaguccg gcgcggaguu cgccuggaug auccgcaagg agcccgggaa caucacgccc     1500

uggaacuucg accagaaggu cgaccggaug gccaccgcca accaguucau caagcgcaug     1560

accaccacgg acaccuaccu gcucggcgag gacgugcugc ccgcgcagag ccugcucuac     1620

cagaaguucg agguccugaa cgagcugaac aagauccgga ucgaccacaa gccgaucucc     1680

aucgagcaga agcagcagau cuucaacgac cucuucaagc aguucaagaa cgugaccauc     1740

aagcaccucc aggacuaccu ggugagccag gggcaguacu ccaagcgccc ccugaucgag     1800

ggccuggccg acgagaagcg guucaacagc ucccugagca ccuacuccga ccugugcggg     1860

aucuucggcg ccaagcucgu cgaggagaac gaccgccagg aggaccugga gaagaucauc     1920

gaguggagca cgaucuucga ggacaagaag aucuaccggg ccaagcugaa cgaccucacc     1980

uggcugaccg acgaccagaa ggagaagcug gccaccaagc gcuaccaggg cugggggcgg     2040

cucucccgca agcugcuggu gggccucaag aacagcgagc accgcaacau cauggacauc     2100

cuguggauca cgaacgagaa cuucaugcag auccaggcgg agcccgacuu cgccaagcug     2160

gugaccgacg ccaacaaggg gaugcucgag aagaccgacu cccaggacgu caucaacgac     2220

cuguacacca gcccccagaa caagaaggcc auccggcaga uccugcucgu ggugcacgac     2280

auccagaacg ccaugcacgg ccaggcgccg gccaagaucc acgucgaguu cgcccgcggg     2340

gaggagcgga acccccgccg guccgugcag cgccagcgcc agguggaggc cgccuacgag     2400

aaggucagca acgagcuggu gagcgcgaag gugcggcagg aguucaagga ggccaucaac     2460

aacaagcggg acuucaagga ccgccuguuc cucuacuuca ugcagggcgg caucgacauc     2520

uacacgggga agcagcugaa caucgaccag cuguccagcu accagaucga ccacauccuc     2580

ccccaggccu ucgucaagga cgacucccug accaaccggg ugcugaccaa cgagaaccag     2640

gugaaggccg acagcguccc caucgacauc uucggcaaga agaugcucuc cguguggggg     2700

cgcaugaagg accagggccu gaucagcaag gggaaguacc ggaaccugac caugaacccg     2760

gagaacaucu ccgcgcacac cgagaacggc uucaucaacc gccagcucgu ggagacgcgg     2820

caggucauca agcuggccgu gaacauccug gccgacgagu acggcgacag cacccagauc     2880

aucuccguga aggccgaccu cagccaccag augcgcgagg acuucgagcu gcugaagaac     2940

cgggacguca acgacuacca ccacgccuuc gacgcguacc ucgccgccuu caucgggaac     3000

uaccugcuga agcgcuaccc caagcucgag uccuacuucg uguacggcga cuucaagaag     3060

uucacccaga aggagaccaa gaugcggcgc uucaacuuca ucuacgaccu gaagcacugc     3120

gaccaggugg ucaacaagga gacgggggag auccugugga ccaaggacga ggacaucaag     3180

uacauccggc acagcuucgc cuacaagaag auccucgugu cccacgaggu gcgcgagaag     3240

cgcggcgccc uguacaacca gaccaucuac aaggcgaagg acgacaaggg cagcgggcag     3300

ggcuccaaga agcugauccg gaucaaggac gacaaggaga ccaagaucua cgggggcuac     3360

agcgggaagu cccucgccua caugacgauc guccagauca ccaagaagaa caaggugagc     3420

uaccgcguga ucggcauccc cacccuggcc cuggcccggc ucaacaagcu ggagaacgac     3480

agcaccgaga acaacggcga gcuguacaag aucaucaagc cccaguucac gcacuacaag     3540

gucgacaaga agaacgggga gaucaucgag accaccgacg acuucaagau cguggugucc     3600

aagguccgcu uccagcagcu caucgacgac gccggccagu ucuucaugcu ggcgagcgac     3660

accuacaaga acaacgccca gcagcuggug aucuccaaca acgcccucaa ggccaucaac     3720

aacacgaaca ucaccgacug cccgcgggac gaccuggagc gccuggacaa ccuccggcug     3780

gacagcgcgu ucgacgagau cgugaagaag auggacaagu acuucuccgc cuacgacgcc     3840

aacaacuucc gcgagaagau ccggaacagc aaccugaucu ucuaccagcu ccccgucgag     3900

gaccaguggg agaacaacaa gaucaccgag cuggggaagc gcaccgugcu gacgcggauc     3960

cuccagggcc ugcacgccaa cgccaccacc accgacaugu ccaucuucaa gaucaagacg     4020

cccuucgggc agcugcgcca gcggagcggc aucucccuca gcgagaacgc gcagcugauc     4080

uaccaguccc cgaccggccu guucgagcgc cgggugcagc ucaacaagau caaguga        4137


<210>  2037
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2037
augaccaacg agaaguacag caucggccug gacaucggga ccuccagcau cggcuucgcc       60

guggucaacg acaacaaccg cgugauccgg gugaagggga agaacgccau cggcguccgc      120

cucuucgacg aggggaaggc ggccgccgac cggcgcuccu uccggacgac ccgccggcgc      180

cugagccggc gccgguggcg ccugaagcuc cugcgggaga ucuucgacgc cuacaucacc      240

cccguggacg aggccuucuu cauccgccug aaggagucca accucagccc gaaggacucc      300

aagaagcagu acagcggcga cauccuguuc aacgaccggu ccgacaagga cuucuacgag      360

aaguacccca ccaucuacca ccuccgcaac gcgcugauga cggagcaccg gaaguucgac      420

gugcgcgaga ucuaccuggc cauccaccac aucaugaagu uccggggcca cuuccugaac      480

gccacccccg ccaacaacuu caaggucggg cgccucaacc uggaggagaa guucgaggag      540

cugaacgaca ucuaccagcg gguguucccc gacgagagca ucgaguuccg caccgacaac      600

cucgagcaga ucaaggaggu gcugcuggac aacaagcgcu cccgggcgga ccgccagcgg      660

acccucguca gcgacaucua ccaguccagc gaggacaagg acaucgagaa gcgcaacaag      720

gccguggcca cggagauccu gaaggccucc cugggcaaca aggccaagcu caacgugauc      780

accaacgucg agguggacaa ggaggcggcc aaggagugga gcaucaccuu cgacuccgag      840

agcaucgacg acgaccuggc caagaucgag gggcagauga ccgacgacgg ccacgagauc      900

aucgaggugc ugcggucccu cuacagcggg aucacgcuga gcgccaucgu cccggagaac      960

cacacccugu cccagagcau gguggccaag uacgaccucc acaaggacca ccugaagcug     1020

uucaagaagc ucaucaacgg caugaccgac accaagaagg cgaagaaccu gcgcgccgcc     1080

uacgacggcu acaucgacgg ggugaagggc aagguccugc cccaggagga cuucuacaag     1140

cagguccagg ugaaccucga cgacuccgcc gaggccaacg agauccagac guacaucgac     1200

caggacaucu ucaugcccaa gcagcggacc aaggcgaacg ggagcauccc ccaccagcug     1260

cagcagcagg agcuggacca gaucaucgag aaccagaagg ccuacuaccc guggcucgcc     1320

gagcugaacc ccaaccccga caagaagcgc cagcagcugg ccaaguacaa gcucgacgag     1380

cuggugaccu uccgggugcc cuacuacgug ggcccgauga ucaccgccaa ggaccagaag     1440

aaccaguccg gcgcggaguu cgccuggaug auccgcaagg agcccgggaa caucacgccc     1500

uggaacuucg accagaaggu cgaccggaug gccaccgcca accaguucau caagcgcaug     1560

accaccacgg acaccuaccu gcucggcgag gacgugcugc ccgcgcagag ccugcucuac     1620

cagaaguucg agguccugaa cgagcugaac aagauccgga ucgaccacaa gccgaucucc     1680

aucgagcaga agcagcagau cuucaacgac cucuucaagc aguucaagaa cgugaccauc     1740

aagcaccucc aggacuaccu ggugagccag gggcaguacu ccaagcgccc ccugaucgag     1800

ggccuggccg acgagaagcg guucaacagc ucccugagca ccuacuccga ccugugcggg     1860

aucuucggcg ccaagcucgu cgaggagaac gaccgccagg aggaccugga gaagaucauc     1920

gaguggagca cgaucuucga ggacaagaag aucuaccggg ccaagcugaa cgaccucacc     1980

uggcugaccg acgaccagaa ggagaagcug gccaccaagc gcuaccaggg cugggggcgg     2040

cucucccgca agcugcuggu gggccucaag aacagcgagc accgcaacau cauggacauc     2100

cuguggauca cgaacgagaa cuucaugcag auccaggcgg agcccgacuu cgccaagcug     2160

gugaccgacg ccaacaaggg gaugcucgag aagaccgacu cccaggacgu caucaacgac     2220

cuguacacca gcccccagaa caagaaggcc auccggcaga uccugcucgu ggugcacgac     2280

auccagaacg ccaugcacgg ccaggcgccg gccaagaucc acgucgaguu cgcccgcggg     2340

gaggagcgga acccccgccg guccgugcag cgccagcgcc agguggaggc cgccuacgag     2400

aaggucagca acgagcuggu gagcgcgaag gugcggcagg aguucaagga ggccaucaac     2460

aacaagcggg acuucaagga ccgccuguuc cucuacuuca ugcagggcgg caucgacauc     2520

uacacgggga agcagcugaa caucgaccag cuguccagcu accagaucga ccacauccuc     2580

ccccaggccu ucgucaagga cgacucccug accaaccggg ugcugaccaa cgagaaccag     2640

gugaaggccg acagcguccc caucgacauc uucggcaaga agaugcucuc cguguggggg     2700

cgcaugaagg accagggccu gaucagcaag gggaaguacc ggaaccugac caugaacccg     2760

gagaacaucu ccgcgcacac cgagaacggc uucaucaacc gccagcucgu ggagacgcgg     2820

caggucauca agcuggccgu gaacauccug gccgacgagu acggcgacag cacccagauc     2880

aucuccguga aggccgaccu cagccaccag augcgcgagg acuucgagcu gcugaagaac     2940

cgggacguca acgacuacca ccacgccuuc gacgcguacc ucgccgccuu caucgggaac     3000

uaccugcuga agcgcuaccc caagcucgag uccuacuucg uguacggcga cuucaagaag     3060

uucacccaga aggagaccaa gaugcggcgc uucaacuuca ucuacgaccu gaagcacugc     3120

gaccaggugg ucaacaagga gacgggggag auccugugga ccaaggacga ggacaucaag     3180

uacauccggc accucuucgc cuacaagaag auccugguga gccacgaggu gcgcgagaag     3240

cgcggcgccc uguacaacca gaccaucuac aaggcgaagg acgacaaggg cuccgggcag     3300

ggcagcaaga agcucauccg gaucaaggac gacaaggaga ccaagaucua cgggggcuac     3360

uccggcaaga gccuggccua caugacgauc guccagauca ccaagaagaa caaggugucc     3420

uaccgcguga ucgggauccc cacccuggcc cucgcccggc ugaacaagcu ggagaacgac     3480

agcaccgaga acaacggcga gcucuacaag aucaucaagc cccaguucac gcacuacaag     3540

gucgacaaga agaacgggga gaucaucgag accaccgacg acuucaagau cguggugagc     3600

aagguccgcu uccagcagcu gaucgacgac gccggccagu ucuucaugcu ggcguccgac     3660

accuacaaga acaacgccca gcagcucgug aucagcaaca acgcccugaa ggccaucaac     3720

aacacgaaca ucaccgacug cccgcgggac gaccuggagc gccucgacaa ccugcggcug     3780

gacuccgcgu ucgacgagau cgugaagaag auggacaagu acuucagcgc cuacgacgcc     3840

aacaacuucc gcgagaagau ccggaacucc aaccucaucu ucuaccagcu gcccgucgag     3900

gaccaguggg agaacaacaa gaucaccgag cuggggaagc gcaccgugcu cacgcggauc     3960

cugcagggcc ugcacgccaa cgccaccacc accgacauga gcaucuucaa gaucaagacg     4020

cccuucgggc agcuccgcca gcgguccggc aucagccugu ccgagaacgc gcagcugauc     4080

uaccagagcc cgaccggccu cuucgagcgc cgggugcagc ugaacaagau caaguga        4137


<210>  2038
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2038
augaagcaga uccugggccu cgaccugggg accaccagca ucggcugggc ccuggugcgc       60

gaggccgaga acaaggacga gcaguccagc aucguccgga ucggggugcg cgugaacccc      120

cucacguccg acgagcagaa gaacuucgag cagggcaaga gcaucaccac caacgcggac      180

cggacccuga agcgcuccau gcggcgcaac cugcagcggu acaagcuccg ccgggaccac      240

cugaaggacu gccugcagaa gaacgggauc aucacggaca aggccguccu cagcgagaac      300

ggcaacuaca ccaccuucca gaccuacuac cugcgcgcca aggccgugaa cgagaagauc      360

ucccuggagg aguucgcccg ggugcuccug augcugaaca agaagcgcgg cuacaagagc      420

ucccggaaga ugaaguccgc ggacgagggg cagcucaucg acggcaugag cgucgccaag      480

cgccuguaca acgacaacau cacgccgggg caguacgugc ugagccuccu ggaggagggc      540

cggaaguaca uccccgacuu cuaccgcucc gaccugcucg acgagcugga caagaucugg      600

accuuccagc agugcuucua ccccgagauc cugacccccg agugccggaa gcucaucgcc      660

gggaagggca agcaggccac cgcgaagauc uuccugggca aguacaagau cuacacggcc      720

gacaacaaga gcaaggacaa gcgccugcag gccuaccagu ggcgggugga ggcccucucc      780

gggcagcugc cgaucgaggu cguggccuuc guggucugcg agaucaacgg cgcgauccag      840

gccagcuccg gguaccuggg cgccaucagc gaccgcucca aggagcucua cuucgagggc      900

cugaccgugg ggcaguaccu ggugaagcag cucgagaaga accccgacac cagccugaag      960

aacaaggucu ucuaccggca ggacuaccug aacgaguucg agaagaucug ggagaagcag     1020

gccgaguacc acaccgagcu cacgccccag cugaagaagg aggugcgcga caucaucauc     1080

uucuaccagc gcacccucaa gagccagaag ggccuggugg gguucugcga guucgagucc     1140

cggcagaucg aggucaugau cgacggcaag cugaagaaga agaccguggg gugccgcgug     1200

ugccccaaga gcuccccgcu guuccaggag uacaagaucu ggcacaaccu caacaaccug     1260

aagaucgucg agaaggagcu gggcaagagc cgguuccuca accaggacga gaaggagucc     1320

cuguuccagg agcugaacau caaggagaag cucagcaaga ccgacauccu gaagcugcug     1380

ugccucaagg ccaaggaguu cgaccugaac uucgagaagg uggagggcaa ccgcacgcag     1440

gcggagcugu uccgggccua ccaggccauc aucgagaugu ccgggaacgg cgacuacgac     1500

uucagcaaga ugaccuccag cgagauccag gacaccguga ccggggucuu ccgcggccuc     1560

ggguacaaca cggacauccu guccuucgac gccgagaagg aggagaagac cuucgacaag     1620

cagcccaugu accugcucug gcaccuccug uacagcuacg agggcgacuc cagcgacacc     1680

ggcaacaagg cccugaucac caagaucaag aagcugugcg gguucgaguc cgaggaguac     1740

gcgcggaucc ugacggacgu gaccuuccag agcgaguacg gcaaccucuc caccaaggcc     1800

auccgccgga uccugcccuu caugaagaag gggagcgagu acuccgaggc cugccgccac     1860

gccggcuacc ggcacagcga gcgcagccug accaaggagg agcucgacaa gaaggucuac     1920

aaggaccacc uggagcugcu cccccggaac ucccugcgca acccgguggu ggagaagauc     1980

cugaaccaga ugauccacgu cgugaacacg guggucgaca ccuacgggaa gcccgacgag     2040

auccggaucg agcucgcccg cgagcugaag aaguccgcga aggagcggga cgagaugacc     2100

gccagcauca acaagaccgc cgccgagcac aagaaguacu gcgaggugcu gcagaaggag     2160

uucggcuuca cgcacgugag ccgcaacgac aucauccggu acaagcucua ccuggagcug     2220

gagaacaacg gcuacaagac ccucuacucc gacaccuaca ucccccgcga gaagcuguuc     2280

agcaaggagu ucgacaucga gcacaucauc ccccagucca agcuguucga cgacagcuuc     2340

uccaacaaga cccucgagug ccgcagcguc aacaucgaga aguccaacga caccgcguac     2400

gacuacgugc ugaacaagua cggggagcag ggcauccugg acuacaagaa ccggaucgag     2460

aaccucuaca aggacagcaa gaucucccgc acgaaguacc agaagcugau gaugaagaac     2520

agcgacaucu ccgggaacuu caucgagcgg gagcugcgcg acagccagua caucgccaag     2580

aaggcccggg agauccucga ggagcuggug aaguucgucg ugccgaccac cggcuccgug     2640

accgcccgcc ugcgggagga cuggcagcuc gucgacguga ugcaggagcu gaacuggaag     2700

aaguacgaca agcugggccu cacggacgug cgcuacgaca aggacgggcg ggagauccgc     2760

uacaucaagg acuggagcaa gcggaacgac caccgccacc acgccaugga cgcgcugacc     2820

aucgccuuca ccaagcggag caucauccag uaccugaaca accucaacgc caaguccgac     2880

aagagcggcu ccaucuacgc caucgagcag aaggagcugu uccgcgacaa gcgggggaag     2940

cugaaguuca ucccgcccau gcccugcaac aucuuccgcg ccgaggcgaa gcggcaccuc     3000

gagaacgucc uggugagcgu gaaggccaag aacaaggucg ugacccccaa caucaaccgc     3060

uccaagggca agaacgggau caacaagaag auccagcuga cgccccgggg ccagcuccac     3120

aacgagacca ucuacggcag caugaagcgc uacgagacca agugggagaa gaucgggucc     3180

ggcuucgacg agcggcagau cgccaaggug gccaacaaga aguuccgcga ggcccugcug     3240

agccgccucg cggaguucgg gaacgacccc cagaaggccu ucaccggcaa gaacucccug     3300

gagaagaacc cccuguacgu cgacgagcuc cacacgcaga aggugccgga gaaggugaag     3360

aucgucaccc uggagaaccu guacaccauc cggaaggaga ucacccccga ccucaagauc     3420

gacaaggugg uggacgccgg gguccgccgg gugcugcagg agcgccugga cgaguucgac     3480

gacgacagca agaaggccuu cuccaaccuc gacgagaacc ccaucuggcu gaacggcaag     3540

aagaugaagc gggugacgau caugggcguc agcaacgcgg ugccccugca cgacaagaag     3600

gacuacaagg ggaaccucgu guucgacucc aagggccgca agcagccggu cgacuucgug     3660

aacaccggga gcaaccacca cguggccauc uaccgggacg aguccggcga gcugcaggac     3720

aucgucguga gcuucuacga ggccaccacc cgcuccacgc uggggauccc cgugaucgac     3780

aaggaguaca agaaggccga gggcuggaac uuccucuuca gcaugaagca gaacgaguac     3840

uucgucuucc ccaacgagga gaccggcuuc aacccgaagg agaucgaccu gaugaacccc     3900

gacaacuaca ccaccaucuc ccccaaccug uaccgggugc agaaguucag caagugcaug     3960

uacgggaaca gcuccgugcg cgaguacguc uuccggcacc accucgagac gagcgugaac     4020

gacuccaagg gccugaagaa caucgccuac aagaacguca agagccuggu guaccucgag     4080

gggaucguga aggugcgcau caaccacauc ggccagaucg ucuccguggg ggaguacuga     4140


<210>  2039
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2039
augcgcaucg acaacggccu ggaggagcuc aaggugaaca accggaucua caacaucggg       60

cuggacaucg gcaccagcuc cgucggguuc gccgugaccg acgcccagga caagcuggug      120

cgcgucaagg gcaagacggc gaucggggug cggcuguucg aggagggcca caccgccgcc      180

gagcgccggg gcuuccgcac cacccggcgc cggcugagcc gccggaagug gcgcaucaac      240

cugcucaacc agaucuucga gcccuacauc uccgagaucg acccgacguu cuucgcccgg      300

cugaagaaca gcucccuggc cgggaaggac aagaaguucg agggcagccu ccuguucccc      360

gaccgcaccg acuaccaguu cuacaaggac aaccccacca uguaccaccu ccggaagcac      420

cucauggaga aggaccgcca guucgaccug cgggagaucu accuggcgau gcaccacauc      480

aucaaguacc gcgggaacuu ccucaacacc acgcccauga accacuucaa gaacgaggag      540

aucgacuucu ccgcccuguu cagccggcug aacgagcucu accaggagau cgacgacgag      600

aacaucuucg agcugaacau cgagaacgug gacgagauca agaagauccu gcucaaccac      660

gaccuguuca agauggagaa gcagaagcag gucgccaagc ugcucaacgu ggacaccgac      720

gacaagcagc ugaagaagga gaacaccgcc cuggcgucca acuucagcaa ggccauccuc      780

ggcuacaagu ucaaccuggc ccuggugacc aagaucccgg accaggacaa gaaggagugg      840

acgaucuccc ucggggacga ggacuucgac gaccgccugg acaacguccc cguggcccug      900

accgagagcc agcucgaggu ggucgaggug cugcacucca ucuucaacag caucucccug      960

aacgagaucg uggagaacgg cgagacccuc agcuccgcca ugguccggaa guacaacgac     1020

cacaagcacg accugaagcu gcucaagaac gugaugacca cgcuggagga ccgcaagaag     1080

gcgaagaaga uccagggcau cuacaacgug uucgucggga aggacaccga ccugaucagc     1140

gacaaggaca agaacaagua caaggacaag gacgaguucu acaaggagau ccagaagaac     1200

cucgacaacu ccagcgacgc cgaggagauc aucaagcgca ucaagcagga ccaguuccug     1260

cccaagcagc ggaccagcaa gaacggcgug aucccccacc agcugcacca gcgcgagcuc     1320

gaccugauca ucgagaacca ggccaaguac uacccguucc ugaaggaggc caaccccaac     1380

aagaagcggc ucaagcaggc caaguacaag cuggacgagc ugaucgcguu caagaucccc     1440

uacuacaucg ggcccaccuc cggcgccgag aacagcgacu ucuccuggcu gaagcgcaag     1500

aaggacggcc agaucacgcc guggaacuuc gacgagaagg uggaccggau cgagagcgcc     1560

aaccgcuuca uccggcgcau gaccaccaag gacaccuacc uccuggccga ggacguccug     1620

ccccucgagu cccugaucua cgagaaguac agcgugcuga acgagcucaa caacgugcgg     1680

aucaaccgcc agaagcuguc cgucaacgac aagcaggaca ucuacaacga gcuguucaag     1740

aacagcaaga cggugacccu caagcagcug cagaaguacu ucgaggucac caagaagcug     1800

aagaccgugg uggucgaggg gcucgccgac cccaagaagu uccuguccaa ccugggcacg     1860

uaccacgacu ucaagcucau cuucggggac aucguggacg accccgagua ccagaccgac     1920

cuggagaaca ucaucgagug gagcaccauc uucgaggacc ggggcaucuu caacuccaag     1980

cugaacgaga ucaccuggcu cacggacgag cagcgcaaga aggugcugag caagcgguac     2040

cagggguggg gccgcuucuc caagaagcug cucgucggcc ugaagaacag ccaggggaac     2100

uccauccugg acgagcucug gaacaccaac cugaacuuca ugcagcugca gagccaggac     2160

gacuucgcgg agcucaucca cgccgagaac gccaagcagu ucaaggccaa cgacccggac     2220

aaccccugga acggcaucga cgccauccug aacgacgcgu acaccagccc ccagaacaag     2280

aaggccaucc gccagguggu gaaggucgug caggacaucg agaaggccau ggggaacaag     2340

ccccagaaga ucgccaucga guucacccgg agcgcggaca agaacccgcg gcgcacggac     2400

ucccggauca agcgccugag cgacaucuac aaggaggccg ccgagaaggu ggucggcgag     2460

agccucaagc uggagcugaa cgaguaccuc accuccaaga agaagaugac cgacaagaug     2520

uaccuguacu ucacccagcu ggggaccgac aucuacacgg gcaagaagau cgacaucgac     2580

cagcucaaca acuacgacau cgaccacgug cugccccaga gcuuccugaa ggacgacucc     2640

cucgacaacc gcgugcugac caaccggagc gacaacuccg agaaguccga caccgucccc     2700

cugcacaagu ucggcgccaa gauggggaac uucuggcgcc agcucgccga cgagggccug     2760

aucaccaagc ggaaguacaa gaaccugacg accgaccccg cgaccaucga caaguacacc     2820

auccaggggu ucauccgccg gcagcucgug gagacguccc aggugaucaa gcugaccgcc     2880

aacauccugg gcgggcucua cccggaggag accgagaucc uggagauccc cgccaagcug     2940

aaccaccagu uccgcgacau guucgaccuc gucaaggugc gggaggugaa cgacuaccac     3000

cacgccuucg acgccuacau caccgucuuc gugggcaacu uccuguaccg ccgguacccc     3060

aagcugcgca gcuacuucgu guacggcgac uucaagaagc ucaacaagac ggacgacgac     3120

auccggaagu ucaacuuccu gaaccuguuc aacaaggaca aggucgagga caacgagacc     3180

gacgagaucc ucugguccaa ggcggagagc auccgcgaca ucaagaacau cuacaacuac     3240

aaguucaugc uggugucccg ggagguguac acccgccacg gggccauguu caaccagacc     3300

aucuaccccg ccagcaagaa cgacuccaag aagcugaucc cgaucaagaa gggcaaggac     3360

acggccaucu acgggggcua cagcggcaac accgacgccu acauggcgau cgucgccgug     3420

gaggacaaga agggggagaa guucaaggug gucggcgugc ccacccgggc cgugucccgc     3480

cucgagcagc uggccgcgga cgaccacgac caguaccuga ccgcccucca cgacguccug     3540

aagcccaacu ucacgaagau caagaccaac cggaagacca aggccaagga ggaggugacc     3600

acgccguucc gcauccuggu ggggaagguc ggcuaccggc agcucaucca ggacggccag     3660

gugaagauga ugcuggggag cuccgccuac aaguacaacg ccaagcagcu ggugcucagc     3720

gagaaguccc ugaaggucau caagaacaac aagaaguaca accccgacga cgagaaccag     3780

gaccugaucg acguguacga cgagauccuc ggcgugguca accgcgcguu caaccuguac     3840

gacaccaacg gguuccggaa gaagcugaac gacaaccgcg ugaaguucgu ggagcucccc     3900

aucaacaaca ucuucgacgg caccaagcug guccagaugg ggaagcgcgc cgugcugacc     3960

cagauccuca acggccugca cgccaacgcc gcccuggggg accucaaggc gcugggcauc     4020

agcacgcccu ucggcaagau gcagagcaag uccgggaucc cgcugagcau ggacgccucc     4080

cucguguacc agagccccac cggccuguuc gagcggaagc ugaaccucuc cgaccuguga     4140


<210>  2040
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2040
augaccaaga agaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucacgg acgacuacaa ggugcccgcc aagaagauga aggugcucgg gaacaccgac      120

aagaaguaca ucaagaagaa ccugcugggc gcgcuccugu ucgacagcgg ggagaccgcc      180

gaggccaccc gccugaagcg gacggcccgc cggcgcuaca cccggcgcaa gaaccggcuc      240

cgcuaccugc aggagaucuu cgccgacgag auggcgaagg ucgacgaguc cuucuuccag      300

cggcuggacg agagcuuccu ccgcugggac gacgacaaca agaagcuggg ccgguacccg      360

aucuucggca acaaggccga cguggugaag uaccaccagg aguuccccac caucuaccac      420

cuccgcaagc accucgccga cuccagcgag aaggccgacc ugcggcuggu cuaccucgcg      480

cuggcccaca ugaucaaguu ccgcgggcac uuccugaucg agggcgagcu caacgccgag      540

aacaccgacg ugcagaagau cuucuccgac uucgugcggg ucuacgaccg cacguucgac      600

gacagccacc uguccgagau caccguggac gccgccagca uccugaccga gaagaucucc      660

aagagccggc gccucgagaa ccugaucaag uacuacccca ccgagaagaa gaacacgcug      720

uucgggaacc ucaucgcgcu ggcccugggc cuccagccca acuucaagau gaacuucaag      780

cuguccgagg acgccaagcu gcaguucagc aaggacuccu acaacgagga ccucgaggag      840

cugcugggga agaucggcga cgacuacgcc gaccucuuca ccagcgccaa gaaccuguac      900

gacgcgaucc ugcucuccgg cauccugacc guggacgaca acagcaccaa ggccccgcug      960

uccgccagca ugaucaagcg guacgccgag caccacgagg accucgagaa gcugaaggag     1020

uucaucaagg ccaacaagag cgagcuguac cacgacaucu ucaaggacga gacgaagaac     1080

ggguacgcgg gcuacaucga gaacgggguc aagcaggacg aguucuacaa guaccucaag     1140

aacacccugu ccaagaucgc cggcagcgac uacuuccugg acaagaucga gcgcgaggac     1200

uuccuccgga agcagcgcac cuucgacaac ggcuccaucc cccaccagau ccaccuccag     1260

gagaugcacg ccauccugcg ccggcagggg gacuacuacc ccuuccugaa ggagaaccag     1320

gaccgcaucg agaagauccu gaccuuccgg auccccuacu acgugggccc gcuggcccgc     1380

aaggacagcc gguucuccug ggccgaguac cacagcgacg agaagaucac gcccuggaac     1440

uucgacaagg ugaucgacaa ggagaagucc gcggagaagu ucaucacccg caugacccug     1500

aacgaccucu accugcccga ggagaagguc cugcccaagc acagccacgu guacgagacc     1560

uacgccgugu acaacgagcu cacgaagauc aaguacguca acgagcaggg gaaggacucc     1620

uucuucgaca gcaacaugaa gcaggagauc uucgaccacg uguucaagga gaaccggaag     1680

gugaccaagg agaagcugcu gaacuaccuc aacaaggagu ucccggagua ccgcaucaag     1740

gaccugaucg gccuggacaa ggagaacaag uccuucaacg ccagccucgg gaccuaccac     1800

gaccugaaga agauccugga caaggccuuc cucgacgaca aggucaacga ggaggugauc     1860

gaggacauca ucaagacccu gacgcuguuc gaggacaagg acaugaucca cgagcggcuc     1920

cagaaguacu ccgacaucuu caccgcggac cagcugaaga agcuggagcg ccggcacuac     1980

accggcuggg gccgccucag cuacaagcug aucaacggga uccggaacaa ggagaacaac     2040

aagaccaucc uggacuaccu caucgacgac ggcuccgcca accgcaacuu caugcagcug     2100

aucaacgacg acacgcugcc cuucaagcag aucauccaga agucccaggu ggucggggac     2160

guggacgaca ucgaggccgu cgugcacgac cuccccggca gccccgccau caagaagggg     2220

auccugcaga gcgugaagau cgucgacgag cuggugaagg ugaugggcga caacccggac     2280

aacaucguca ucgagauggc ccgggagaac cagaccacca accgcggccg gucccagagc     2340

cagcagcgcc ucaagaagcu gcagaacagc cugaaggagc ucggguccaa cauccugaac     2400

gaggagaagc ccagcuacau cgaggacaag guggagaacu cccaccucca gaacgaccag     2460

cuguuccugu acuacaucca gaacggcaag gacauguaca ccggggacga gcuggacauc     2520

gaccaccuga gcgacuacga caucgaccac aucauccccc aggcguucau caaggacgac     2580

uccaucgaca accgggugcu gacgagcucc gccaagaacc gcggcaagag cgacgacguc     2640

cccucccugg acaucgugcg cgcccggaag gccgaguggg ugcgccucua caagagcggg     2700

cugaucucca agcggaaguu cgacaaccug accaaggccg agcgcggcgg ccucaccgag     2760

gcggacaagg ccggguucau caagcggcag cuggucgaga cccgccagau caccaagcac     2820

guggcccaga uccuggacgc ccgguucaac acggagagcg acgagaacga caaggugauc     2880

cgcgacguca aggugaucac ccucaagucc aaccugguga gccaguuccg gaaggacuuc     2940

gaguucuaca agguccgcga gaucaacgac uaccaccacg cccacgacgc guaccugaac     3000

gccguggugg gcaccgcccu ccugaagaag uacccgaagc uggccuccga guucgucuac     3060

ggggaguaca agaaguacga cgugcacaag cucaucgcca agagcuccga cgaccacagc     3120

gagaugggca aggcgaccgc caaguacuuc uucuacagca accugaugaa cuucuucaag     3180

cgggugaucc gcuacuccaa cggcaagguc aucgugcggc ccguggucga guacagcaag     3240

gacacggagg acaucgccug ggacaagaag uccaacuucc gcaccaucug caaggugcug     3300

agcuaccccc aggugaacau cgucaagaag guggagaccc agaccggggg cuucuccaag     3360

gagagcaucc uccccaaggg ggacuccgac aagcugaucc cgcggaagac gaagaaggcc     3420

uacugggaca ccaagaagua cggcggguuc gacagcccca ccguggcgua cuccgucuuc     3480

gugguggccg acgucgagaa gggcaaggcc aagaagcuga agaccgugaa ggagcucgug     3540

ggcaucagca ucauggagcg cuccuucuuc gaggagaacc ccgucgaguu ccuggagaac     3600

aagggguacc acaacauccg ggaggacaag cugaucaagc uccccaagua cagccuguuc     3660

gaguucgagg gcgggaagcg ccggcugcuc gccuccgcca gcgagcugca gaagggcaac     3720

gagaugguga ucccggggca ccuggugaag cuccuguacc acgcgcagcg caucaacucc     3780

uucaacagca cgaaguaccu ggacuacguc uccgcccaca agaaggaguu cgagaaggug     3840

cucagcugcg uggaggacuu cgccaaccug uacgucgacg uggagaagaa ccuguccaag     3900

auccgggccg uggccgacag cauggacaac uucagcaucg aggagaucuc caacagcuuc     3960

aucaaccucc ugacccugac cgcgcucggc gcccccgccg acuucaacuu ccugggcgag     4020

aagauccccc gcaagcgcua caccuccacg aaggagugcc ugaacgccac ccucauccac     4080

cagagcauca ccgggcugua cgagacccgg aucgaccugu ccaagaucgg cgaggaguga     4140


<210>  2041
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2041
augggcaagu acaaccuggg gcucgacauc ggcaccaaca gcguggggug ggccgucgug       60

gacgagaaca accagaucgu gaagaagggc gggaaggccc uguggggcgu ccgcauguuc      120

gacgaggcgc aguccgccaa ggagacccgg aucuaccgca acagccggcg ccggaucgug      180

cgccggaacc agcgccugcu ccugcugcag aaggaguucu acgacgagau caucaaggug      240

gaccagacgu ucuuccagcg gcucaaggac uccuucuuca agaaggagga caagcugaac      300

aagaacaacu acaccuucuu cgacgacgac aucaccgacc gcgaguacuu caagaaguuc      360

cccaccaucu accaccuccg gagccaccuc cuguacaacg acgagaagau cgacauccgc      420

augcuguacc uggcccugca ccacaucguc aaguaccggg gccacuuccu ggacuccggg      480

gaggugaacg agagcaacuu cuccaagauc aaggacgugc ucgacacguu caacaacaag      540

augagcgaga ugucccugga guucgaggac aacgaggacu acuucugcaa gaucgaguuc      600

gacgagaacu ucgagaacaa gcugaaggac aucaugcuca gccagcuguc caagaaggag      660

aagcgccagg agcugaucga ccucuucaag gucgacaaga agucccuggu gaacgagcug      720

cucaucgagc ugcuggugaa caagaagguc aagcucaaca gccugagcau caccaacggc      780

aagaaguacg aggugugcga gaucgacuuc gagaaggagg agcuguucga gaagcucgag      840

gacgccaaga agaucaucac cgagcuguuc gacauccugg acgagaucgu gaacaucaag      900

aagaucgucg acuacuacua cgugcucaag aucaucgggg acaacgagua ccuguccgag      960

gccaugguga agaaguacga caagcacgac aaggaccugc gggagcucaa gagcuucauc     1020

aagaccuacu gcccgaacaa guacuacgag ugcuuccgca agacguccaa ccccaaccug     1080

ugcaacuacc ccaaguacgu cggcaucaac agcgugaaca agaagaucga gcgguucgcg     1140

cacugcaaga aggaggaguu cuacaaguac cugaaguccc ucuucaacac caucaaggac     1200

gagaacgcca agcugaagau cgaccuguuc cagaaggaca uggagaacga cgacuuccuc     1260

aaccgccaga acagcaacca caacagcucc auccccaugc agcugaacuu cugcgagcug     1320

aagaagaucc ucgagaacca gagcaaguac uacccguucc ugaugcugaa gaacuccgac     1380

ggguacagca ccuccgaccg gaucaucgcc aucuucaagu acaagauccc cuacuacgug     1440

ggcccccuca acaagaacag caaguacucc uggcugguca agaaguccaa cgagaacauc     1500

acccccugga acuacgagaa ggugaucgac cuggacgcca gcgcgaagga guucauccag     1560

cgcaugcaga acaagugcac guaccugaag ggcgagaacg acuacugccu cccgaagaag     1620

uccauccugu ucagcgagua caacugccug caguaccuca accggcugau caucaacggg     1680

gcccugaucg agaacaagcu caagaacgag aucuacgaga acaucuuccu gugcuacaag     1740

aagcccacca agaaguccau cguggaguac cugaagacca acuacaacua cgacgagacc     1800

acgcucaaga gcaacauccu ggaggucaac ugcgacauga gcuccuacau ccaguucaag     1860

gagaucuacg gcgaggacuu cgagcagaac cugaacaucg uggaggacau caucaaggac     1920

aucaccaucu ucgaggacaa gaagauccuc gagaagcgcc ugcgcgagau cuaccaccug     1980

agcgaggaca agguccagaa gaucaagggg cucaacuaca agggcuacgc cacccugucc     2040

aagcacuucc ugaacaacuu cuacagcgug aacgaggaga ccggcgagau cuacgggccc     2100

aucauccaga ucaugcggga cacgaacuac aaccuccagc aggucaucua caacgaccgc     2160

uacaacuuca ugaaggccau cgacaaguac aacaagaaga accuggacac cgagaagaag     2220

cugaacgugu ccgacuucau cgacgaguac aucuacguga gccccaucau gaagcggagc     2280

cucauccagu ccuacaacau cauccaggag cuggagaaga uccugggcga gagcaucucc     2340

aaguacuaca ucgagugcgc ccgcgugcgg agcaacucca aggacgucau caacagccgc     2400

uaccggcagc ucuccgagcu guacaaggac ugcgagcacc aggugagcga caucuccaag     2460

aacuacauca acaucaacaa gcugaagaag cagcucgagg acaacaagaa cgagcugcgc     2520

gcggacaagc uguaccucua cuucacccag uucgggaagu gcauguacac ccugaaggac     2580

aucgaccugg acgacguguu ccucggcaag uacgacaucg accacaucua cccgcagagc     2640

cugaucaagg acgacucccu gaacaaccgg guccucgcca gcaaggagaa gaacgggaag     2700

aaggacaacc aguucaucuu caacaucccc gaguuccugc ccaagaacgc cgagcuguuc     2760

uacaagaagc uccuggagug caagcugauc acgaaggaga aguaccgccg gcucaccaug     2820

aagaagaucg acgacgacga gcuggacauc uucgugaacc gccagcuggu ggccaccaac     2880

caguccguca agggccucgu ggacgugcug aagaucuaca agggcgucaa gcccaccaac     2940

aucguguaca gcaaggccgu gaacaucucc gacuuccgga acaacuacaa ccuguacaag     3000

agccgcacgg cgaacaacua ccaccacgcc cacgacgccu accucaacgu cgugaucggg     3060

aaggcccugg acguguacua ccagugcaac uacuuccaga acuacaagga cgucaucaag     3120

cugaaggacc ggaacaucac caucaacccg gagaucaucc ucuccaagaa ccgcuacgug     3180

aaccggaacg gcgagaagaa ggugaucugg gaccuggaca acacccugaa gcucaucaag     3240

aacaaccugu acaaccgcuu cgacaucacc gagacgauca ugacccgcaa gagcaacaag     3300

auguucagca aguccagcau cucccccgcc aagaacagca agaaccugau ccccaucaag     3360

accuccgacc cccggugcaa cguccucaag uacgggggca ucaagagcaa cuccuacaac     3420

uucuacugca ucguggagcu guacgacaag aaggggaaga aguccuacau ccuggaggcg     3480

aucccgaaga cccacagcga caagcucgag gaguacauca agaacagcau cgacgagaag     3540

aacgugucca acuucaagau caucaacaag aacaucaaga gcaacaucau caucgaguac     3600

aagaagcugc gcuacgucau cacgggcaag uccggcaagu acuaccugau caagaacuuc     3660

aaggaccgga acuucagcua caacgccauc aagaccauca aguacaucga gaaguacaac     3720

uaccagaaga aguacgagau ccccuucgag gagaccaagg agaagaucau ccucgccccc     3780

aagaagaagg acaccaagga gaucucccug acccuggaca acugccaggc ccuccuggac     3840

gagaucauca gccuguucaa gaaggacauc uacaacuacg acaucaucua caagaucacg     3900

uccaagcucg aggagaacaa gcagaaccug acccugacca accucaucaa ggugcugacc     3960

gagauccuga uccuccugaa gacgaacgag cgcgggaccg ccgaccugac ccucaucgac     4020

augagcaagg acaccggcac gcugugcauc uccaagaccc ugaagcccgg gaugaaguuc     4080

aucagcgaga gcccgaccgg cuacuaccgg aagaucaucu ucgaggugcc caacgggauc     4140

uga                                                                   4143


<210>  2042
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2042
augaagcgca uccugggccu cgaccugggg accaccagca ucggcugggc ccuggugaac       60

gaggccgaga acaacaacga ggcguccagc aucguccggc ucggggugcg cgugaacccc      120

cugacggucg acgagaaguc caacuucgag aagggcaagg ccaucaccac caacgccgac      180

cggcagcugc gccacggggc ccggaucaac cuccagcgcu acaagcugcg gcgccagaac      240

cugcacgacu gccuccagaa gcagggcugg cugggcaccg aggccaugua cgaggagggg      300

aaggcgagca cguucgagac cuacaagcug cgggccaagg ccgccgagga ggagaucucc      360

cuccacgagu ucgcgcgcgu gcuguucaug cugaacaaga agcggggcua caagagcaac      420

cgcaaggcca acaacaagga ggacgggcag cuguucgacg gcaugaccau cgccaagaag      480

cuguacgagg agcaccugac cccggccgag uacucccucc agcugcugaa caaggggaag      540

aaguucacgc agggcuacua ccggagcgac cucaacgccg agcuggagcg caucugggac      600

gagcagaaga aguacuaccc cgagauccug accgacgagu ucaagcagca gcucgagggc      660

aagaccaaga ccaacacguc caagaucuuc cuggcgaagu acgggaucua cagcgccgac      720

cugaagggcc ucgaccggaa guuccagccc cugaaguggc gcguggaggc ccugcagcag      780

caggucgaca aggaggugcu cgccuucgug aucuccgacc ugaaggggca gaucgccaac      840

accagcggcc ugcucggcgc gaucuccgac cggagcaagg agcuguacuu caacaagcag      900

accgucgggc aguaccugug ggccucccuc gaggagaacc cccacaucag caucaagaac      960

aagccguucu accgccagga cuaccuggac gaguucgaga agaucuggga gacccaggcc     1020

gccuuccaca agcagcugac gcccgagcuc aagcaggaga uccgggacau caucaucuuc     1080

uaccagcgcc cccugaaguc caagaagucc cugaucagcg ugugcgagcu cgagcagcgg     1140

aaggugaagg ccaccaucga cggcaaggag aaggagauca ccaucgggcc caaggucgcg     1200

cccaagagca gcccgguguu ccaggaguuc cgcaucuggc agaaccugaa caacgugcug     1260

cucaucgaca acgacaccaa cgagaagcgc ccccuggacg aggucgagcg gaaccugcuc     1320

uacaaggagc uguccaucaa ggccaagcug agcaagacgg aggcccugaa gauccucaac     1380

aagaagggca agcaguggga ccugaacuac cgcgagcugg aggggaaccg gacccaggcc     1440

auccucuucg acugcuacaa ccgcaucauc acccugaccg gccacgagga gugcgacuuc     1500

aagaagauca aggccuccga gauccggcac uacgugagca cgaucuucaa gaaccugggc     1560

uucuccaccg agauccucga cuucgacccc agccugaaga agcacgagcu ggagaagcag     1620

ccgauguacc agcucuggca ccuccuguac uccuacgaga gcgacaacuc ccgcaccggg     1680

aacgagagcc uccugcggaa gcuggagacc acguucggcu uccccgagga guacgcgacc     1740

gugcucugcg acgucguguu cgaggaggac uacgggaacc uguccgugaa ggccaugcgc     1800

gagauccugc ccuaccucca ggccggcaac gacuacagcc aggccugcgc guacgccggg     1860

uacaaccacu cccggcacag ccugaccaag gaggagcugg accagaaggu cuacaaggag     1920

cgccucgagc ugcugcccaa gaacucccuc cggaacccgg ugguggagaa gauccugaac     1980

cagaugauca acgucaucaa cgccaucauc gacgaguacg gcaagcccga cgagauccgc     2040

aucgagaugg cccgggagcu caagagcucc gccgcggacc gcaagaagac cacgcacgcc     2100

aucagccagg gcaacgccga gaaccagcgg auccgcgaga uccuggagaa ggaguucucc     2160

cugagcuaca ucagccggaa cgacaucauc aaguacaagc uguacgagga gcucgagccc     2220

aacuacuaca agacccugua cuccgacacc uacaucacca aggacaagcu guucagcaag     2280

gacuucgaca ucgagcacau cauccccaag gcccgccucu ucgacgacuc cuucagcaac     2340

aagacgcugg aggcccggaa caucaaccug gagaagucca acaagaccgc guucgacuuc     2400

aucaaggaga aguacgggga ggacggcgcc gaggccuaca agaagaagcu cgacaugcug     2460

cuggagaacg acgccaucag ccgcccgaag uacaacaacc uccugcgcgc cgaggcggac     2520

auccccuccg acuucaucaa ccgggaccug cgcaacaccc aguacaucgc caagaaggcc     2580

ugcgagaucc ucggggagcu ggugaagacc gucaccccca cgaccggcaa gaucaccaac     2640

cggcugcgcg aggacuggca gcucguggac gugaugaagg agcugaacuu cgagaaguac     2700

gagaagcugg ggcucaccga gaucgucgag gaccgggacg gccgcaagau caagcggauc     2760

aaggacugga cgaagcgcaa cgaccaccgg caccacgcca uggacgcgcu ggccaucgcc     2820

uucaccaagc ccagcuucau ccaguaccug aacaaccuca acgcccgcuc caacaagggc     2880

gacagcaucu acgccaucga gaacaaggag cugcacuacg aggaggggaa gcugcgguuc     2940

aacgcgccga uccccgugaa cgaguuccgc gccgaggcca agcggcaccu cuccgccauc     3000

cuggugagca ucaaggccaa gaacaagguc augacccaga acgugaacaa gaucaagacc     3060

aagcacggca ucaucaagaa gauccagcug acgccccgcg ggccccucca caacgagacc     3120

aucuacggca ccaagaugcg gccgaucauc aagaugguga aggucggcgc ggcccuggac     3180

gaggccacca ucaacaaggu guccagcccc gccauccgcg aggcccugcu caagcggcug     3240

aacgaguacu ccgggaacgc gaagaaggcc uucacgggca agaacacccu ggagaagaac     3300

cccaucuacc ucaacgccgg gcgcaccaag accgugccga gccuggucaa gacgguggag     3360

ugggaguccu uccaccccac ccggaagcug aucgacaagg accucaacgu ggacaagguc     3420

guggacaagg gcauccgcga gauccugaag gcccggcugg aggaguucaa cggggacgcg     3480

aagaaggccu ucagcaaccu cgaggagaac cccaucuacc uggacgaggc caagaagauc     3540

gcccugaagc gcgugagcau cgagggcguc cucuccgcca ucccccugca cacccugaag     3600

aaccaggcgg gcaagccgau caccgggaag gacggcaagc ccgugcucgg gaacuacgug     3660

cagacgagca acaaccacca caucgccuuc uacuacgacg aggacggcaa ccugcaggac     3720

aacgccgucu ccuucuucga ggccgccgag cgcaagagcc aggggauccc cgugaucgac     3780

aaggacuaca accgggacaa gggcuggcgc uuccuguuca ccaugaagca gaacgaguac     3840

uucguguucc ccaacgaggc gaccggcuuc aucccguccg aggucgaccu caccgacgag     3900

gccaacuacg ggaucaucag ccccaaccug uaccgggugc agaagguguc ccgcaucgac     3960

aagggcacga gcgccucccg ggacuacugg uuccgccacc accuggagac cauccucaac     4020

gacgacgcca agcugaagaa ccuggccuuc aagcggaucc gcgggcuccu ggagcugaag     4080

gacaucauca agguccggau caacagcacc ggcaagaucg uggcgguggg ggaguacgac     4140

uga                                                                   4143


<210>  2043
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2043
augaagaaga uccugggccu cgaccugggg accaccagca ucggcugggc ccuggugaac       60

gaggccgaga cggaguccga gaaguccagc aucauccgcc ucgggguccg ggugaacccc      120

cugaccaucg acgagaaggg caacuucgag aaggggaagg cgaucaccac caacagcgac      180

cgccagcagc ggcacuccgc ccgcaucaac cugcagcggu acaagcuccg ccggcagaac      240

cugugcgacu gccugcagau cggcggccuc cuggggagcg aguccaugua cgaggagggc      300

aaggagagca cguucgagac cuacaagcug cgcgccaagg ccgccaccga gaagguggcg      360

cuccacgagu ucgcccggau ccuguucaug cugaacaaga agcgcgggua caaguccaac      420

cggaaggcca acagcaagga ggacggccag gccuucgacg ggaugaccau cgcgaagaag      480

cucuacgagg agaaccugac gccggccgag uacucccugc agcuccugaa caagggcaag      540

aaguucagcc ccuccuacua ccgcagcgac cuggaguccg agcucaaccg gaucugggag      600

gagcagaaga aguacuaccc cgagauccug accgacgacu ucuaccagca gcuggagggc      660

aagaccaagg ucaacaccac gaagaucuuc cucgccaagu acgggaucua caccgccgac      720

cugaagggcc uggacaagaa gaugcagccc cucaaguggc gcaucgaggc ccugaacaag      780

caggucgaca aggaggugcu gaccuucgug gugagcgacc ucaaggggca gaucgagaag      840

accuccggcc ugcugggcga gaucagcgac cgguccaagg agcucuacuu caacaagcag      900

acgguggggc aguacgucug ggcgagccug aaggagaacc cgcacgugag caucaagaac      960

aagcccuucu accgccagga cuaccuggac gaguucgaga agaucuggga gacccaggcc     1020

gccuuccaca aggagcucac ccccgagcug aagaaggaga uccgggacau caucaucuuc     1080

uaccagcgcc cccugaaguc caagaagggc cucaucagca ucugcgagcu ggagcagaag     1140

aaggagaagg ugaucaucga ggggaaggag aaggagaucc ugaucggcuc caaggucgcc     1200

cccaagagcu cccccaugua ccaggaguuc cggaucuggc agaaccucaa caacgugcug     1260

cugaucgaca ccgagaucaa cgagagccgc ccccucgacg aggaggagaa ggcccugcug     1320

uacaacgagc ucuccgugcg ggcgaagcug agcgagacgg aggcccugaa gaccaucaac     1380

aagaagggga agcacuggga ccugaacuuc aaggagcucg agggcaaccg cacccaggcc     1440

guccuguucg agugcuacaa ccgcaucauc acccagacgg gccacgagga gugcaacuuc     1500

aagaagaucu ccgccgagga cauccggcac uacguggcca ccaucuucaa gaaccugggg     1560

uucaacaccg acauccucga cuucaacccc agccuggaga agcacgagcu ggagaagcag     1620

ccgauguacc agcucuggca ccuccuguac uccuacgaga gcgacaaguc ccgcaccggc     1680

aacgagagcc ugcugaacaa gcuggaggcg aacuucgggu ucccccagga guacgccucc     1740

gugcucgccg acgccacguu cgaggacgac uacggcaaca ucagcgucaa ggccauccgg     1800

aagauccugc cccaccucca cgacggguac gacuacuccg aggcgugcga gcgcgccggc     1860

uacaaccaca gcgugcgguc ccugaccaag gaggagcugg cccagaaggu guacaaggac     1920

cgccuggacc uccugcccaa gaacagccug cggaacccgg ucguggagaa gauccucaac     1980

cagaugauca acgugaucaa cgccaucauc gucgaguacg gcaagcccga cgagauccgc     2040

aucgagaugg cgcgggagcu caagagcucc gccgccaagc gcgaggagcg gaccaagagc     2100

aucaaccaga acgccgccga gaacaagcgc aucaccgaga uccugcagac ggaguucggg     2160

cuguccuaca ucagccggaa cgacaucgug aaguacaagc uguaccagga gcuggaggcg     2220

aacggcuuca agacccucua cuccaacacc uacaucccca aggagaagcu guucagcaag     2280

gacuucgaca ucgagcacau cguccccaag gcccgccugu ucgacgacuc cuucgggaac     2340

aagaccaucg aggcccggga cgugaaccuc gccaagagca acaagacggc cuucgacuuc     2400

gugaaggaga aguacggcga gcaggggauc gaggaguaca agaagaagcu ggacacccug     2460

cucaaggaga acgcgaucuc cagcggcaag uacaagaagc ugaugauguc cgaggccgac     2520

aucccgagcg acuucaucaa ccgcgaccug cggaacaccc aguacaucgc ccgcaaggcc     2580

uacgagaucc ucggcgagcu ggucaagacc aucaccccca cgaccgggaa gaucaccgac     2640

cggcugcgcg aggacuggca gcucaucgac gugaugaagg agcugaacuu cgagaaguac     2700

gaccgccugg gccucaccga ggugaucgag gaccgggacg ggcgcaagau caagcggauc     2760

aaggacugga cgaagcgcaa cgaccaccgg caccacgcca uggacgcgcu gaccaucgcc     2820

uucaccaagc ccuccuacau ccaguaccug aacaaccuca acgcccgcag cgacaagucc     2880

ggcagcaucu acgccaucga gaccaaggag cugcaccggg agaacaacac gcugcgcuuc     2940

aacgcgccca ugccgcucaa cgaguuccgg aaggaggcca agcgccaccu guccgccauc     3000

cuggucagca ucaaggccaa gaacaaggug augacccaga acgugaacaa gaucaagacc     3060

aagaacggca ucaucaagaa gguccagcuc accccgcgcg gggcccugca caacgagacg     3120

aucuacggca ccaagaugcg gcccaucauc aagaugguga agaucggggc ggcccuggac     3180

gaggccacca ucaacaaggu gagcuccccc accauccggg aggcccuccu gaagcgccug     3240

aacgaguaca gcggcgacgc caagaaggcg uucgccggga agaacgccau cacgaagaac     3300

ccgaucuacc ucaacgccga gcacaccaag accgcccccg cgauggucaa gaucguggag     3360

ugggagaugu acuaccccau ccggaagcug aucaccccgg accugaagau cgagaaggug     3420

aucgacaacg gcaucaagga gauccucaag aagcgccuga aggaguucgg cggggacgcc     3480

aagaaggccu ucuccaaccu ggacgagugc cccaucuacc ucgacgagga gaagaacauc     3540

acgcugaagc gggucagcau cgagggcgug cuguccgcca uccccgugca cacccucaag     3600

aaccacgcgg ggaaggagau caaggacgag gacggcaagg ccguccuggg ggacuacgug     3660

cagaccagca acaaccacca cguggccauc uaccaggacg ccaagggcaa ccugcaggac     3720

aacgucaucu ccuucuucga ggccgcggag cgcaagagcc agaacgugcc caucguggac     3780

aagaaccuca accgggacaa gggcuggcac uuccuguaca ccaugaagca gaacgaguac     3840

uucgucuucc cgaacgagga gacgggguuc gugcccuccg agaucgaccu gauggacgag     3900

aacaacuacg agaugaucag ccccaaccuc uuccgcgugc agaccauguc ccgggucgac     3960

uacggcaaga ccaucauccg cgaguacgug uuccgcaacc accuggagac cauccugaac     4020

gacaaccuca agcugaaggg gcugguguuc aagaagauca agagccucaa ggcccuggag     4080

ggcaucguca aggugcgggu gaacgccauc gggaagaucg ucgccguggg cgaguacgac     4140

uga                                                                   4143


<210>  2044
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2044
augaagaaga aguacgccau cggcaucgac aucgggacca acagcguggg cugguccguc       60

gugaccgacg acuacaaggu gcccagcaag aagaugaagg ucuucgggaa cacggagaag      120

cgcuacauca agaagaaccu gcucggcacc cugcuguucg acgaggggaa caccgccgag      180

aaccggcgcc ucaagcggac cgcgcgccgg cgcuacacgc ggcgccggaa ccgcauccug      240

uaccugcagg agaucuucgc cgaggagauc aacaagaucg acgacuccuu cuuccagcgg      300

cucgacgaca gcuuccugau cguggaggac aagcagggcu ccaagcaccc gaucuucggc      360

acccugcagg aggagaagga guaccacaag caguucccca ccaucuacca ccuccgcaag      420

cagcuggccg acagcuccca gaaggccgac auccggcuga ucuaccucgc ccuggcgcac      480

aucaucaagu accgcgggca cuuccuguuc gagggcgacc ucaagagcga gaacaaggac      540

gugcagcacc uguucaacga cuucgucgag auguucgaca agaccgugga gggguccuac      600

cugagcgaga accuccccaa cguggccgac guccuggugg agaagguguc caagagccgg      660

caccuggaga acauccucca cuacuucccc aacgagaaga agaacggccu guucgggaac      720

uuccuggccc ucgcccuggg ccugcagccg aacuucaaga cgaacuucga gcucgcggag      780

gacgccaaga uccaguucuc caaggagacc uacgaggagg accuggagga gcugcucggc      840

aagaucgggg acgacuacgc cgaccuguuc aucgccacca agagccugua cgacggcauc      900

cuccuggccg ggauccuguc caccacgaac agcaccacca aggcgccccu cuccagcucc      960

auggucaacc gcuacgagga gcaccagaag gaccuggccc ugcucaagaa cuucauccac     1020

cagaaccuga gcgacagcua caaggaggug uucaacgaca agcugaagga cggcuacgcc     1080

ggcuacaucg aggggaagac cacgcaggag aacuucuacc gguucaucaa gaaggccauc     1140

gagaagaucg acggcuccaa cuacuucauc gagaagaucg agcgcgagga cuuccuccgg     1200

aagcagcgca ccuucgacaa cgggagcauc ccccaccaga uccaccuccg ggagaugcag     1260

gccaucaucc gccgccaggc gaaguucuac cccuuccugg aggagaacca ggacaagauc     1320

gagaagaucc ucaccuuccg gaucccguac uacgugggcc cccuggcccg cgggaagucc     1380

gaguucgccu ggcugaaccg caagagcgac gagaagaucc ggcccuggaa cuucgacgag     1440

auggucgaca aggagaccuc cgccgagaac uucaucacgc ggaugaccaa cuacgaccag     1500

uaccuccccg accagaaggu gcugccgaag cacagccugc uguacgagaa guucgccgug     1560

uacaacgagc ucaccaaggu ccgcuacgug accgagcagg gcaaguccuu cuucuucgac     1620

gcgaacauga agcaggagau cuucgaccgg acguucaagg uguaccgcaa ggucaccaag     1680

gagaagcuga uggacuuccu gagcaaggag uucgacgagu uccggaucgu ggaccuccug     1740

ggccuggaca aggacaacaa guccuucaac gccagccucg ggaccuacca cgaccugaag     1800

aagaucgugu ccgaggacuu ccuggacaac agcgagaacg aggacauccu cgagaacguc     1860

gugcugaccc ugacgcuguu cgaggaccgc gagaugaucc ggaagcgccu ggagaaguac     1920

aaggacgucc ugaccgagga gcagcggaag aagcucgagc gccggcacua caccggcugg     1980

gggcgccugu ccgccaagcu gaucaacggc auccgggaca aggugacccg caagacgauc     2040

cucgacuacc ugaucgacga cgggaccagc aaccggaacu ucaugcagcu gaucaacgac     2100

gacacccucu ccuucgugga cgagauccgc cuggcccagg gcagcggcaa ggccgaggac     2160

uaccgcgcgg agguccagaa ccuggccggg ucccccgcca ucaagaaggg cauccuccag     2220

agccugaaga ucguggacga gcugaucgag gugauggggu acgaccccga gcacaucguc     2280

guggagaugg cccgggagaa ccaguucacc aaccagggcc gccggaacuc ccagcagcgc     2340

uacaagaaga ucgagaacgc gaucaagaac cucgacagca accugaacag caagauccug     2400

aaggaguacc ccacgaacaa ccaggcccuc cagaacgacc ggcuguuccu guacuaccuc     2460

cagaacggga aggacaugua caccgacgag gagcuggaca ucgaccagcu gucccaguac     2520

gacaucgacc acaucauccc gcaggccuuc aucaaggacg acagccucga caacaaggug     2580

cugaccaagu ccgccaagaa ccgcggcaag agcgacgacg uccccucccu ggagaucgug     2640

uacaagaaga agaacuucug gaagcagcuc cuggacagcc agcugaucuc ccagcggaag     2700

uucgacaacc ucaccaaggc cgagcgcggc gggcugacca acgaggacaa ggcgcgguuc     2760

auccagcgcc agcuggugga gacgcggcag aucaccaagc acgucgcccg cauccucgac     2820

acccgguuca acaccaagcu ggacgaggcc ggcaaccgca uccgggaccc caaggugaac     2880

aucaugacgc ucaagagcuc ccucgugagc caguuccgca aggacuacca gcuguacaag     2940

guccgggaga ucaacaacua ccaccacgcc cacgacgccu accugaacgc ggugguggcc     3000

accgcccucc ugaagaagua cccccagcug gccccggagu ucgucuacgg ggacuacccc     3060

aaguacaacu ccuacaagag ccgcaagucc gccaccgaga aggugcuguu cuacagcaac     3120

aucaugaacu ucuucaagcg ggugcugguc uacuccaaga ccggcgaggu gcgcauccgg     3180

cccgugaucg aggucaacaa ggagacgggc gagaucgugu gggacaagaa guccgacuuc     3240

cgcaccgugc gcaagguccu gagcuacccc caggugaacg uggucaagaa gaccgaggug     3300

cagaaccacg ggcucgaccg gggcaagccg aagggguucu acaacgcgaa ccugagcccc     3360

cgccccaagg agggcagcgu ggagaaccug guccccgcca agcaguccuu cgacaccaag     3420

cgguacgggg gcuacgccgg caucagcaac uccuacgccg ugcucgugaa cgggaucauc     3480

gagaagggca agaagaaguc caaggcggag gucacggagu uccaggggau cagcauccug     3540

gcccgcaagg acuucgacaa gaacccgaag caguaccugc ucaaccuggg cuacaaggac     3600

aucaagagca ucaucaagcu gcccaaguac ucccucuucg agcuggagaa cgggagccgg     3660

cgcaugcugg ccuccauccu cagcaccaac aacaagcggg gcgagaucca caagggcaac     3720

gagauguacc ugcccgacaa guucgugacc cugcucuacc acgccaugcg cgugaaccgg     3780

acccuggagc cggggcacaa gaaguacguc gaggcccacc gccacguguu cgacgagcug     3840

cucacguaca uccuggagua caacgagaag aucgugggcg cgaaggccaa cgggaagcgg     3900

aucgucgagg ccuacuccgc ccgcaaggac accgacaacc uggaggagcu cugcaacagc     3960

uucaucaacc ugcugaaccu caccgcccug ggcuccgcgg uggacuucga guuccugggg     4020

accaagaucc cccgguaccg cgacuacacg cccagcuccc uccugaaggc cacccugauc     4080

caccagagcg ugaccggccu cuacgagacc cggaucgacc uguccaagcu gggcggggac     4140

uga                                                                   4143


<210>  2045
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2045
augaagaaga uccugggccu cgaccugggg accaccagca ucggcugggc ccugguguac       60

gaguccgaga acgccgacga gaaguccucc aucguccgcc ucggggugcg ggugaacccc      120

cugagcgucg acgagaaggg caacuucgag aaggggaagu ccaucacgac caacgcggac      180

cgccagcaga cccacggcgc ccggaucaac cugcagcgcu acaagcuccg gcgcgagaac      240

cugaaggacu gccugcagaa gcagggcugg aucgggaccg aggcccucua cgaggagggc      300

aagacgagca ccuucgagac cuacaugcug cgggccaagg ccgcggagga ggagaucucc      360

cugcacgagc ucugccgcgu gcuguucaug cugaacaaga agcgggggua caagagcaac      420

cgcaaggcca acaacaagga ggacggcaag cuguucgacg ggaugaccgu ggccaagaag      480

cuguacgagg agcaccugac gccggccgag uacucccuca agcugcugag cuccggcaag      540

aaguucaagc aggaguacua ccggagcgac cucaaggagg aguucgaccg caucuggaac      600

gagcagcaga aguacuaccc cgagauccug accgacgacu ucaagcagca gcugcagggc      660

aaguccaagg ggaacaccag caagaucuuc cucggcaagu acgggaucua cuccgcggac      720

cugaagggcc uggaccggaa gcuccagccc cugaaguggc gcgucgaggc ccugaagcag      780

aagguggaca aggaggugcu cgccuucguc aucagcgacc ugaacggcca gaucgccucc      840

accagcgggc ugcucggcgc caucuccgac cggagcaagg agcuguacuu caacaagcag      900

acgguggggc aguaccugug gaccagccuc aaggagagcc cccacgugag caucaagaac      960

aagccguucu accgccagga cuaccuggac gaguucgaga agaucuggga gacccaggcg     1020

accuaccaca aggagcugac gcccgagcuc aagcaggaga uccgggacau caucaucuuc     1080

uaccagcgcc cccugaaguc caagaagggc cugaucagcg ucugcgagcu cgagcagcgg     1140

aaggugaagg ugaccaucga cgggaaggag aaggagaucu ccaucggccc caaggucgcc     1200

cccaagagcu cccccauguu ccaggaguuc cgcaucuggc agaaccugaa caacauccug     1260

cucaucgaca agggcaacaa cagcaaccgg ccccuguacg aggaggagcg caaccugcuc     1320

uacgaggagc uguccgugaa ggagaagcug agcaaggccg aggcccugaa ggugcucaac     1380

aagaacggga agcaguggga ccugaacuac aaggagaucg agggcaaccg cacccaggcc     1440

guccuguacg acugcuacaa ccggaucauc gggaugaccg gccacgagga gugcaacuuc     1500

aagaagcuca aggcguccga cauccgcuac aacgugaaga cgaucuucaa gaaccugggg     1560

uucagcaccg acauccugga cuucgacucc agccuccagg gccacgagcu ggagcggcag     1620

cccuccuacc agcuguggca ccuccuguac agcuacgagu ccgacaacag ccgcaccggc     1680

aacgacgccc ugcuccggaa gcuggagacc gccuucucca ucccgaagga guacgccgcc     1740

gugcugagcg acgucguguu cgaggacagc uacgggaacc ucuccgugaa ggcgaugcgc     1800

aacauccugc cccaccucca ggccggccuc gacuacagcc aggccugcga gaaggccggg     1860

uacaagcacu cccggcgcag ccugacgaag aacgagcugg acaagaagga guacaaggag     1920

aagcucgagc ugcugcccaa guccagccuc cacaaccccg ucguggagaa gauccugaac     1980

cagaugauca acgucaucaa cgccgugauc gacgaguacg gcaagccgga cgagauccgg     2040

aucgagcugg cgcgcgagcu caagaucggg gccgccgagc gggagaacau gacccgcucc     2100

aucaaccagg gcaacgccga gaacaagcgg auccgcgaga uccuggagaa ggaguucgcg     2160

cugagcuaca ucucccggaa cgacaucauc aaguacaagc ucuacgagga gcucaagagc     2220

aacggcuuca agacccugua cuccgacacc uacaucagca aggacaagcu guucuccaag     2280

gacuucgaca ucgagcacau cauccccaag gcccgccugu ucgacgacag ccuguccaac     2340

aagacgaucg aggcccggga cgugaacauc gccaagagca acaagaccgc cuucgacuuc     2400

aucuccgaga aguacgacca gcaggggauc gaggcguaca agaagaagcu cgacauccug     2460

cugcaggacg gcagcaucag caaggccaag uacaaccgcc uccugaugaa ggaggccgac     2520

auccccuccg gguucaucga ccgggaccug cgcgacaccc aguacaucgc caagaaggcc     2580

uacgagaucc ucagcgaccu gguccgcaac gugaccccca cgaccggcgc ggugaccucc     2640

cggcugcgcg aggacuggca gcucgucgac gugaugaagg agcugaacuu cgagaaguac     2700

cagaagcugg gccucgugga ggucaucgag gaccgggacg ggcgcaagau caagcggauc     2760

aaggacugga ccaagcgcaa cgaccaccgg caccacgcca uggacgcccu gacgaucgcc     2820

uucaccaagc cgagcuacau ccaguaccug aacaaccuca acgcccgcuc cgacaagaac     2880

agcuccaucu acggcaucga gaacaaggag cugcaccggg aggacaacaa gcugcgcuuc     2940

aacgcgcccc uccccaucaa cgaguuccgg gccgaggccc gccggcaccu caagagcauc     3000

cugguguccg ugaaggccaa gaacaagguc augacccaga acgugaacgu gaucaagacc     3060

aagcacgggg ucaucaagaa ggugcagcug accccgcgcg gcgagcugca caacgcgacg     3120

uucuacggga ccaagaagcg gccgaucauc aagaugguga aggucggcgc cgcccuggac     3180

gaggugacca ucaagaaggu gagcuccagc uucauccgcg aggcccuccu ggaccggcug     3240

cacgaguaca acggcgacgc caagaaggcg uucaccgggc gcaacuccau cgacaagaac     3300

cccaucuaca ucgacgagga gcacaagaag acgguccccg cccucgugaa gaccaucgag     3360

ugggagaugu accaccccac ccggaagaug aucagcccgg accugaagau cgacaaggug     3420

aucgacaagg gcauccgcga gauccugcgg acccgccucg ccaaguucaa cggggacgcc     3480

cagaaggccu ucuccaaccu ggacgagaac cccaucuaca aggacgaggc gaagaagauc     3540

acgcugaagc gcgucagccu cgagggcgug uccagcguga ucccccugca caagcugaag     3600

gacaugggcg ggaagccggu cgugggcaag gacgggaagc ccgugugggg cgacuacguc     3660

cagaccucca acaaccacca caucgccuuc uacauggacg agaacgggaa ccuccaggac     3720

gacgugguga ccuucuucaa ggccgcccgg cgcaagagcc agggcguccc cgugaucgac     3780

aagaacuaca accacgacaa gggguggcac uuccuguuca ccaugaagca gaacgaguac     3840

uucguguucc ccaacgaggc cacgggcuuc aucccgaccg aggucgaccu gauggacgag     3900

gcgaacuacg acaugaucag ccccaaccuc uaccgggugc agaagauguc caagaccaag     3960

cagggcauca ccgacaagcg cgacuacugg uuccggcacc accuggagac ggugcugaac     4020

aacgaccuca agguccgcaa cagcaucuuc aagcagauca acucccugaa gccccuggag     4080

gggaucguga aggugcggau caacgccauc ggcaagaucg ucgccaucgg ggaguacgac     4140

uga                                                                   4143


<210>  2046
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2046
augaagcgca uccugggccu cgaccugggg accaccagca ucggcugggc ccuggugaac       60

gaggccgagu ccaacaacga ggcgagcucc aucguccggc ucggggugcg cgugaacccc      120

cugacggucg acgagaaguc caacuucgag aagggcaagg ccaucaccac caacgccgac      180

cggcagcugc gccacggggc ccggaucaac cuccagcgcu acaagcugcg gcgccagaac      240

cugcacgacu gccuccagaa gcagggcugg cugggcaccg aggccaugua cgaggagggg      300

aaggcgagca cguucgagac cuacaagcug cgggccaagg ccgccgagga ggagaucagc      360

cuccacgagu ucgcgcgcgu gcuguucaug cugaacaaga agcggggcua caaguccaac      420

cgcaaggcca acaacaagga ggacgggcag cuguucgacg gcaugaccau cgccaagaag      480

cuguacgagg agcaccugac cccggccgag uacagccucc agcugcugaa caaggggaag      540

aaguucacgc agggcuacua ccgguccgac cucaacgccg agcuggagcg caucugggac      600

gagcagaaga aguacuaccc cgagauccug accgacgagu ucaagcagca gcucgagggc      660

aagaccaaga ccaacacgag caagaucuuc cuggcgaagu acgggaucua cuccgccgac      720

cugaagggcc ucgaccggaa gcugcagccc cugaaguggc gcguggaggc ccucaagcag      780

caggucgaca aggaggugcu ggccuucgug aucagcgacc ugaaggggca gaucgccaac      840

accuccggcc uccugggcgc gaucagcgac cgguccaagg agcuguacuu caacaagcag      900

accgucgggc aguaccucug ggccagccug gaggagaacc cccacaucuc caucaagaac      960

aagccguucu accgccagga cuaccuggac gaguucgaga agaucuggga gacccaggcc     1020

acguuccaca agcagcucac ccccgagcug aagcaggaga uccgggacau caucaucuuc     1080

uaccagcgcc cccucaagag caagaagucc cucaucagcg ugugcgagcu ggagcagcgg     1140

aaggugaagg ccaccaucga cggcaaggag aaggagauca ccaucgggcc caaggucgcc     1200

cccaagagcu cccccguguu ccaggaguuc cgcaucuggc agaaccugaa caacgugcuc     1260

cugaucgaca acgacacgaa cgagaagcgc ccccuggacg aggacgagcg guaccuccug     1320

uacaaggagc ugagcaucaa ggcgaagcug uccaagaccg aggcccucaa gauccugaac     1380

aagaagggca agcaguggga ccugaacuac aaggagcucg aggggaaccg cacccaggcc     1440

auccuguucg acugcuacaa ccggaucauc acccugacgg gccacgagga gugcgacuuc     1500

aagaagauca aggccagcga gauccgccac uacgucucca ccaucuucaa gaaccucggc     1560

uucagcaccg agauccugga cuucgacccc ucccuggaga agcacgagcu cgagaagcag     1620

ccgauguacc agcuguggca ccuccucuac agcuacgagu ccgacaacag ccggaccggg     1680

aacgaguccc ugcugcgcaa gcucgagacg accuucggcu uccccgagga guacgccacc     1740

gugcugugcg acguggucuu cgaggaggac uacgggaacc ugagcgugaa ggcgaugcgg     1800

aagauccucc ccuaccugca ggccggcaac gacuacuccc aggccugcgc cuacgcgggg     1860

uacaaccaca gccgccacuc ccugaccaag gaggagcucg accagaaggu guacaaggag     1920

cggcuggagc ugcuccccaa gaacagccug cgcaacccgg ucguggagaa gauccugaac     1980

cagaugauca acgugaucaa cgccaucauc gacgaguacg gcaagcccga cgagauccgg     2040

aucgagaugg cccgcgagcu caaguccagc gccgccgacc ggaagaagac gacccacgcg     2100

aucaaccagg gcaacgacga gaaccagugc auccgcgaga uccuggagaa ggaguucgcc     2160

cuguccuaca ucagccggaa cgacaucauc aaguacaagc ucuacgagga gcugaagccc     2220

aacggguuca agacccugua cagcgacacc uacaucccca aggacaagcu guucuccaag     2280

gacuucgaca ucgagcacau caucccgaag gcccgccugu ucgacgacag cuacuccaac     2340

aagacgcugg aggcccggga caucaaccuc gccaagagca acaagaccgc guucgacuuc     2400

gucaaggaga aguacggcga gcagggggcc gaggccuaca agaagaagcu ggacaugcug     2460

cucggcaaca acgugaucuc caagcccaag uacaacaagc ugcugaugac cgaggccgac     2520

auccccagcg acuucaucaa ccgcgaccuc cggaacaccc aguacaucgc ccgcaaggcg     2580

ugcgagaucc ugggggagcu gguccgcacc gugacgccca ccaccggcaa gaucaccaac     2640

cggcuccgcg aggacuggca gcugguggac gucaugaagg agcugaacuu cgagaaguac     2700

gagaagcucg gccugacgga gaucguggag gaccgggacg ggcgcaagau caagcggauc     2760

aaggacugga ccaagcgcaa cgaccaccgg caccacgcca uggacgcccu ggccaucgcg     2820

uucaccaagc cguccuacau ccaguaccuc aacaaccuga acgcccgcag caacaagggc     2880

gacuccaucu acgccaucga gaacaaggag cugcacuacg aggaggggaa gcuccgguuc     2940

aacgccccca uccccgugaa cgucuuccgc gccgaggcga agcggcaccu gagcgccauc     3000

cuggugucca ucaaggccaa gaacaaggug augacccaga acgucaacaa gaucaagacg     3060

aagcacggca ucaucaagaa gauccagcuc accccgcgcg gcccgcugca caacgagacc     3120

aucuacggga ccaagaugcg gcccaucauc aagaagguga aggugggcgc cgcccuggac     3180

gaggcgacga ucaacaaggu cagcuccccc gccauccgcg aggugcuccu gaagcggcug     3240

aacgaguaca gcgggaacgc caagaaggcc uucaccggca agaacacccu cgacaagaac     3300

ccgaucuacc ugaacgccga gcgcaccaag acggugcccg cguccgucaa gaccguggag     3360

ugggagaacu accaccccau ccggaagccc aucaccccgg accugaaggu ggacaagguc     3420

aucgacaagg ggaucggcga gauccucaag gcccgccugg aggaguucaa cggcgacgcc     3480

aagaaggccu ucagcaaccu ggaggagaac cccaucuacc ucgaccagac caagaagauc     3540

gcgcugaagc ggguguccau cgagggggug cugagcacga ucccccucca cacccugaag     3600

aaccaggccg gcaagcccau cauggggaag gacggcaagc cgguccuggg gaacuacgug     3660

cagaccagca acaaccacca caucgccuuc uacuacgacg aggacggcaa ccuccaggac     3720

aacgccgugu ccuucuucga ggccgcggag cgcaagagcc agggcaucuc cgucaucgac     3780

aaggacuaca accgggacaa gggguggcgc uuccuguuca ccaugaagca gaacgaguac     3840

uucguguucc ccaacgaggc cacgggcuuc auccccagcg agguggaccu gaccgacgag     3900

gccaacuacg ggaucaucuc ccccaaccuc uaccgcgucc agaagaugag ccggaucgag     3960

aagggcaccu ccgugagccg cgacuacugg uuccggcacc accuggagac cauccugaac     4020

gacgacuccc gccucaagaa cagcgccuuc aagcggauca acucccugaa gccgcuggag     4080

gggaucauca aggugcgcau caacagcauc ggcaagaucg ucgccguggg cgaguacgac     4140

uga                                                                   4143


<210>  2047
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2047
augaagaagg gcuacagcau cgggcuggac aucggcacca acuccguggg guucgccguc       60

aucaccgacg acuacaaggu gcccagcaag aagaugaagg ugcucggcaa cacggacaag      120

cgcuucauca agaagaaccu gaucggggcc cugcuguucg acgagggcac caccgcggag      180

gcccggcgcc ugaagcggac cgcccgccgg cgcuacacgc ggcgcaagaa ccggcugcgc      240

uaccuccagg agaucuucuc cgaggagaug agcaaggucg acuccagcuu cuuccaccgg      300

cuggacgacu ccuuccugau cccggaggac aagcgcgaga gcaaguaccc caucuucgcc      360

acccucgagg aggagaagga guaccacaag aaguucccca ccaucuacca ccuccggaag      420

caccuggccg acuccaagga gaaggcggac cuccgccuga ucuaccuggc ccucgcccac      480

augaucaagu accggggcca cuuccuguac gaggacaccu ucgacaucaa gaacaacgac      540

auccagaaga ucuucaacga guucaucagc aucuacgaca acgccuucga gggguccagc      600

cuguccggcc agaacgccca gguggaggcc aucuucacgg acaagaucag caaguccgcc      660

aagcgcgagc gggugcucaa gcuguucccc gacgagaagu ccaccgggcu guucagcgag      720

uuccucaagc ugaucgucgg caaccaggcc gacuucaaga agcacuucga ccuggaggac      780

aaggccccgc uccaguucag caaggacacc uacgacgagg accuggagaa ccugcucggg      840

cagaucggcg acgacuucgc ggaccuguuc guggccgcca agaagcugua cgacgccauc      900

cuccuguccg gcauccugac cgugacggac cccagcacca aggcgccccu cagcgcgucc      960

augaucgagc gcuacgagaa ccaccagaac gaccuggucg ugcugaagca guucaucaag     1020

aacaaccucc ccgagaacua cguggagguc uucagcgacg acuccaagaa cggguacgcc     1080

ggcuacgugg ggguggacag caagauccgg aagcgcaacg ccaagcuggc caccgaggag     1140

gaguucuaca aguaccugaa gaacauccuc aacaacguca agggcgccga cuacuuccug     1200

gacaagaucg agcgggagga cuuccugcgc aagcagcgca ccuucgacaa cggcuccauc     1260

ccgcaccaga uccaccucca ggagaugaac gcgauccugc ggcgccaggg ggagcacuac     1320

cccuuccuga aggagaaccg ggagaagauc gagaagaucc ucacguuccg cauccccuac     1380

uacgugggcc cccuggcccg ggggaaccgc gacuucgccu ggcugacccg gaacagcgac     1440

caggccaucc gcccguggaa cuucgaggag aucguggaca aggccuccag cgcggaggac     1500

uucaucaaca agaugaccaa cuacgaccuc uaccugcccg aggagaaggu ccugcccaag     1560

cacucccugc ucuacgagaa guucgccgug uacaacgagc ugaccaaggu gaaguucauc     1620

gccgagggcc ugcgggacua ccaguuccuc gacagcgggc agaagaagca gaucgucaac     1680

cagcuguuca aggagaagcg caaggugacg gagaaggaca ucauccacua ccugcacacc     1740

guggacggcu acgacggcau cgagcucaag gggaucgaga agcaguucaa cgccucccug     1800

agcaccuacc acgaccugcu caagaucauc aaggacaagg aguucaugga cgaccccaag     1860

aacgaggaga uccuggagaa caucguccac acccugacga ucuucgagga ccgggagaug     1920

aucaagcagc gccucgcgca guacgacucc cuguucgacg agaaggugau caagagccug     1980

acccggcgcc acuacaccgg cugggggaag cucuccgcca ugcugaucaa cggcauccgg     2040

gacaagcaga ccgggaagac gauccuggac uaccucaucg acgacgccua cuacaaccgc     2100

aacuucaugc agcugaucaa cgacgacggc cugagcuuca aggagaucau ccagaaggcc     2160

caggucgugg gcaagaccga cgacgugaag caggucgugc aggagcuccc gggguccccc     2220

gccaucaaga agggcauccu gcagagcauc aagaucgugg acgagcuggu caagaucaug     2280

cggcacaacc ccgaguccau cgugaucgag auggcgcgcg agaaccagac caccgcccgg     2340

gggaagaaga acagccagca gcgcuacaag cgcaucgagg acgcccucaa gaaccuggcc     2400

cccggccugg acagcaacau ccucaaggag cacccgacgg acaacgugca gcugcagaac     2460

gaccggcugu uccucuacua ccugcagaac gggaaggaca uguacaccgg cgaggcccug     2520

gacaucaacc agcucuccag cuacgacauc gaccacauca ucccccaggc guucaucaag     2580

gacgacuccu ucgacaaccg cguccugacc agcuccaagg acaaccgggg caagagcgac     2640

aacgugcccu ccaucgaggu gguccagaag cgcaaggccu ucuggcagca gcugcucgac     2700

agcaagcuga ucuccgagcg gaaguucaac aaccugacca aggccgagcg cgggggccuc     2760

gacgagcggg acaagguggg guucaucaag cgccagcugg uggagacgcg gcagaucacc     2820

aagcacgucg cccagauccu ggacgcccgc uacaacaccg aggugaacga gaaggacaag     2880

aagaaccgga ccgugaagau caucacccuc aagagcaacc uggucuccaa cuuccgcaag     2940

gaguuccggc uguacaaggu gcgcgagauc aacgacuacc accacgcgca cgacgccuac     3000

cucaacgccg uggucgccaa ggcgauccug aagaaguacc ccaagcugga gccggaguuc     3060

guguacggcg aguaccagaa guacgaccuc aagcgguaca ucagccgcuc caaggacccc     3120

aaggaggugg agaaggccac ggagaaguac uucuucuaca gcaaccugcu gaacuucuuc     3180

aaggaggagg uccacuacgc cgacggcacc aucgugaagc gggagaacau cgaguacucc     3240

aaggacaccg gggagaucgc cuggaacaag gagaaggacu ucgccaucau caagaaggug     3300

cucagccugc cccaggucaa caucgugaag aagcgcgagg ugcagaccgg cggguucucc     3360

aaggagagca uccugcccaa gggcaacagc gacaagcuca ucccgcggaa gacgaaggac     3420

auccuguggg acaccaccaa guacgggggc uucgacuccc ccgucaucgc guacagcauc     3480

cugcucaucg ccgacaucga gaagggcaag gccaagaagc ugaagaccgu gaagacgcug     3540

guggggauca ccaucaugga gaaggccgcc uucgaggaca accccaucac cuuccucgag     3600

aacaagggcu accacaacgu ccgcaaggag aacauccugu gccugccgaa guacucccuc     3660

uucgagcugg agaacgggcg gcgccgccug cucgcgagcg ccaaggagcu gcagaagggc     3720

aacgagaucg ugcugcccgu guaccucacc acgcugcugu accacuccaa gaacguccac     3780

aagcucgacg agcccgggca ccuggaguac auccagaagc accggaacga guucaaggac     3840

cugcucaacc uggugagcga guucucccag aaguacgugc uggccgacgc caaccucgag     3900

aagaucaaga gccuguacgc cgacaacgag caggcggaca ucgagauccu ggccaacucc     3960

uucaucaacc uccugaccuu caccgcccug ggcgcccccg cggccuucaa guucuucggc     4020

aaggacaucg accgcaagcg guacaccacg gucagcgaga uccucaacgc cacccugauc     4080

caccagucca ucaccgggcu guacgagacc cgcaucgacc ucagcaagcu gggcgaggac     4140

uga                                                                   4143


<210>  2048
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2048
augaaccugc gccacguggg caacuacaac aucgggcucg acaugggcac cgccagcguc       60

gggugggccg cgaucgacga gcagggcgag cugcugcacu ucaaggggaa gcccaccugg      120

ggcucccgcc ucuucgacag cgccaacacg gccgccaccg cccggaugcc gcggggccag      180

cgccggcgcu acauccggcg ccgguggcgc cuggaccugc uccagaaguu cuucuccgag      240

gagauggcgg agaaggaccc cgaguucuuc auccggcugc gccagagcuc ccuguggccc      300

gaggaccggg agggcgggca caccgccuac cacuggcccc ucuucaacga cagcgacuuc      360

aacgagaagg aguacuacga gaaguucccg accaucuucc accuccgcca guggcugaug      420

gagacggacg agaaggccga cauccggcuc aucuaccugg ccuuccacaa caucgugaag      480

caccgcggga acuuccugca ggagaacaac gacucccuca gcgcgcagaa cgccaacaug      540

cgggaguccc uggagagcuu cugcaucgcc cuggaggagu ggugcgacga gaaggagcug      600

accugcuccu gcgaccccca gaagcuggag caccuguuca aggaggugaa guaccgcagc      660

gacauccggg acggcuucgc cgcccuccug gggcuggcgu gcuccggcga gaacgagguc      720

gacgugaagg ccauggccaa ggagaugggc aaggccaucg ugggguacaa ggccgacuuc      780

agcaagaucu ucaccgagug cgacaaggag ggcuccagcu uccgccucuc cgacgacgag      840

gggcccgagg cguuccugga caucugcccc gacgacggca ucgcccuguu cgaggccauc      900

cagauggucu acagcuccuu cauccucacc ggcaugcuga cggagaaggg gcugagccuc      960

agcaagaucu ccgccuacaa ccaguacggc caggaccugg agagccugaa ggcccucgug     1020

aaggaguacg ugccgaacga guacuccagc uucuuccggg ggcccaccua cuccggcagc     1080

accgaguacg accccuccaa ggcgcagggg uacacccgcu acaaccuggg ccccagcaag     1140

aagggcuccg gggccaagcc gauggccuac gaggacuuca agaaguccgu cgagcagcug     1200

uucgccaaca cgaacgccau caacgacgag cgcuacaagg agaugauggg ccgguucgcg     1260

gaggagaagu uccuccgccg gcugcgcacc agcgacaacg ggcggauccc cuuccagcug     1320

aaccucgagg agaugaccgc caucaucauc aaccagggca aguacuaccc cuuccugcug     1380

gagcagcagg agaagcucga gagccuggug cgcuuccgga uccccuacua cguggggccg     1440

cugacccgca ucaacgcccg gcucgacucc aagggcaacg ccugcuucgc guggagcaag     1500

cgcaaggacg gcauggacuc cgccagcauc aagcccugga acugggacga ggucaucgac     1560

aagcacgccu ccgcccaggc cuucaucgag cggaugacgg ggaccugcac cuaccugcgc     1620

ggcgagcccg ugcugccccg gugcagccuc cuguacgagg aguacugcgu gcugaacgag     1680

cugaacgggg cgcgcuggac ccaggacggc gacaaguuca uccgguucga cuacaaggac     1740

cgcuccgaca ucaucgagga gcuguucaag aagcggcgcg ggcggaucca guacgccgcc     1800

gucgccgacu ggaugaagca ggagcgcagc cacgccaacg ugcacgugag cggcggccag     1860

ggggagaacg cguucgaguc ccgccucagc uccuaccacu ucuucugcaa cgacgucuuc     1920

ggcguggacg agcugccggg gagcuacgug gacauggucg aggagaucau ccucuggucc     1980

acgcuguucg aggaccggag cauccugcgc gaggagaucc agcggaagua cggcgacgug     2040

cucaacgagg agcagaucaa gaccaucugc aagaagcgcu ucuccggcug ggggcggcug     2100

agccgcaagc ugcucugcuc ccugaaggcc cagaccgaca acggccccaa gagcaucaug     2160

gacauccugc aggaggggga ccagaacaac ggccgggugg ggcgcgccau ggucuuccag     2220

gagauccucc acgacgagca gcugggcuuc gagcagaaga ucgaggagga gaacacccag     2280

uccaugcugg agggcgagaa ccuccugauc gaggaccugc ccgggagccc cgccauccgg     2340

cgcuccauca accaggccgu gcggaucguc gacgagaucg ugggcaucgc ggggcacgcc     2400

ccggacaaca ucuucaucga ggugacgcgc gaggacgaca ccaagaagaa gggcaagcgg     2460

accaccacgc gcuacagcaa cauccagaag gcccucgagg cccugaagga ggagggggcg     2520

gaggcccugc gggagcucaa gaccuacaag cacaacgagc uggacaagcg ccugucccuc     2580

uacuucaugc agaacggcaa gagccuguac uccggcaucc cccuggacau ccggcgccuc     2640

agcgaguacc agaucgacca caucaucccc cagagcuaca ucaaggacga cuccuacgag     2700

aacauggccc ugguccugcc cggggagaac cagagcaagg ccgacaaccu ccugaucucc     2760

caggacaucc ggaucaagau gaaggccacc uggaccgcgc ugcacaaggc cggccucaug     2820

ggggacaaga aguacaacaa ccugaugaac agcuccauca gcgacgccaa gaugaagggc     2880

uucaucaacc gccagcuggu ggagacgucc cagagcguga agcucaccca gaugaugcug     2940

ucccagaagu acccgggggc caagguccgg cccaucaagg ccagcaucuc cagccagcug     3000

cgcgacgcgu gcgaccucgu gaagugccgc gaggccaacg acuaccacca cgcccacgac     3060

gccuaccugg ccugccaggu gggccgguuc guccugaagc gccacuccag cauguucgac     3120

aaccccaucg gcaugaccaa ggugauccgg gacuucguga agcgccaggg gcaggaguuc     3180

aagcggaacg gcaagcuccc ggcguacgcc ucccgcagcg gguucaucgu ccaguccuuc     3240

augcggagcg gcuucgacgc cgagaccggg gagcugcugc gcgacgacug gaacgccccc     3300

uucgagaucg cgcggaucaa gcgcugccuc aacuaccggg acugcuacau cucccgcaug     3360

cccauggagg acagcggcgc cuucugggac gccacgaucu acagccccaa ggccggcaag     3420

aagggggccc ugccggugaa gcagggccug cccguggaga aguacggguc cuacagccgg     3480

gagcaguucg cguacuucuu cgucuacaag gccuacaacc ccaagaagaa ggccuaccgc     3540

cucgaguucg aggccgugcc cgugcggguc gccuccgcgg uggagacccg cgagaacgcc     3600

cuggaggagu acgccaagag cguggccaag caggccggcc uggaguucgu cgagguggcg     3660

cggccgaagg uguacaagua ccagcucauc gagcugggcg aggaccgccu guacaucacc     3720

gggaagcggg agguccgcaa cgccucccag cucgccuuca gccuggacga gaucgaccug     3780

cucaagcgcc uggccgaggg cgaggagacc uccagcgagg agcgggagcg ccuguucgac     3840

gugcucaucg agaaguacgc ccgguacgcg ccccgccugg cccggcagcu guccguggac     3900

agcauguccg ccaccuucga gacgcucaac gccgaggagc agcuggggau ccugcucagc     3960

cuggucgaga ucgcgaacgg caagcgcaac augaucgacc uguccagcgc cgggggcucc     4020

aagaacgccg gcugcaugaa ccccaccuuc agcuccgagc ucagcucccc cgggagcaac     4080

uucaccuuca ucgaccagag cgugaccggc auguucgagc ggcgccagcg gcuggagcug     4140

uga                                                                   4143


<210>  2049
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2049
auggugaaga agaaguacgc caucggcauc gacaucggga ccaacagcgu cggcuggucc       60

aucgugaccg acgacuacaa ggugcccagc aagaagauga aggucuucgg gaacacggag      120

aagcgcuaca ucaagaagaa ccugcugggc acccugcugu ucgacgaggg gaacaccgcc      180

gagaaccggc gccucaagcg gaccgcgcgc cggcgcuaca cgcggcgccg gaaccgcauc      240

cuguaccugc aggagaucuu cgccgaggag aucaacaaga ucgacgacuc cuucuuccag      300

cggcucgacg acagcuuccu gaucguggag gacaagcagg gcuccaagca cccgaucuuc      360

ggcacccugc aggaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacagcuc ccagaaggcc gacauccggc ugaucuaccu cgcccuggcg      480

cacaucauca aguaccgcgg gcacuuccug uucgagggcg accucaagag cgagaacaag      540

gacgugcagc accuguucaa cgacuucguc gagauguucg acaagaccgu ggaggggucc      600

uaccugagcg agaaccuccc caacguggcc gacguccugg uggagaaggu guccaagagc      660

cggcgccugg agaacauccu ccacuacuuc cccaacgaga agaagaacgg ccuguucggg      720

aacuuccugg cccucgcccu gggccugcag ccgaacuuca agacgaacuu cgagcucgcg      780

gaggacgcca agauccaguu cuccaaggag accuacgagg aggaccugga ggagcugcuc      840

ggcaagaucg gggacgacua cgccgaccug uucaucgcca ccaagagccu guacgacggc      900

auccuccugg ccgggauccu guccaccacg gacagcacca ccaaggcgcc ccucuccagc      960

uccaugguca accgguacga ggagcaccag aaggaccugg cccugcucaa gaacuucauc     1020

caccagaacc ugagcgacag cuacaaggag guguucaacg acaagcugaa ggacggcuac     1080

gccggcuaca ucgaggggaa gaccacgcag gagaacuucu accgcuucau caagaaggcc     1140

aucgagaaga ucgagggcuc caacuacuuc aucgacaaga ucgaccggga ggacuuccuc     1200

cgcaagcagc ggaccuucga caacgggagc aucccccacc agauccaccu ccaggagaug     1260

cacgccauca uccgccgcca ggcggaguuc uaccccuucc ugguggagaa ccaggacaag     1320

aucgagaaga uccucaccuu ccggaucccg uacuacgucg gcccccuggc ccgcgggaag     1380

uccgaguucg ccuggcugaa ccgcaagagc gacgagaaga uccggcccug gaacuucgac     1440

gagauggugg acaaggagac cuccgccgag aacuucauca cgcggaugac caacuacgac     1500

cgcuaccucc ccgaccagaa ggugcugccg aagcacagcc ugcuguacga gaaguucgcc     1560

gucuacaacg agcucaccaa ggugaaguuc aucgcggagg gcaugcggga cuaccaguuc     1620

cuggacuccg gccagaagaa ggacaucgug aagacccugu ucaagacgaa gcgcaagguc     1680

accgccaagg acaucaaggc cuaccucgag aacagcaacg gguacgccgg cguggagcug     1740

aaggggcugg aggagcaguu caacgccucc cuccccaccu accacgaccu gcugaagauc     1800

cuccgggaca aggcguucau cgacgccgag gagaaccagg agauccugga ggacaucgug     1860

cugacccuca cgcuguucga ggaccgcgag augauccgga agcgccugga gaaguacaag     1920

gacauccuca ccgaggagca gcggaagaag cuggagcgcc ggcacuacac cggcuggggg     1980

cgccugagcg ccaagcucau caacggcauc cuggacaagg ucacccggaa gacgauccug     2040

ggcuaccuca ucgacgacgg gaccuccaac cgcaacuuca ugcagcugau caacgacgac     2100

acccugagcu ucguggacga gauccggcuc gcccagggcu ccggggaggc ggaggacuac     2160

cgcgccgagg uccagaaccu ggccggcagc cccgccauca agaaggggau ccugcagucc     2220

cucaagaucg uggacgagcu gaucgaggug augggcuacg agcccgagca caucgucgug     2280

gagauggccc gcgagaacca guucaccaac cagggccggc gcaacagcca gcagcggauc     2340

aaccgccugg agaacgcgcu caaggacuuc ggguccaaga uccuggagga gcagaagccg     2400

uacggcguga gcaaggucaa caacauccac cuccaguccg accggcuguu ccuguacuac     2460

cugcagaacg ggaaggacau guacacggac gaggagcucg acaucgacca ccugagcgag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacagccu ggacaacaag     2580

gugcucacca aguccgccaa gaaccgcggc aagagcgacg acgugcccuc cauggagauc     2640

guccacaacc ggaagaacuu cuggcgccag cugcucaaga gcaagcugau cucccagcgg     2700

aaguucgaca accugaccaa ggccgagcgc ggcgggcuga ccaacgagga caaggcccgg     2760

uucauccagc gccagcucgu ggagacccgg cagaucacga agcacguggc gcgcaugcug     2820

gacacccggu ucaacaccaa gcuggacgag gccggcaacc gcauccggga ccccaagguc     2880

aacaucauca cccucaagag caaccuggug ucccaguucc gcaaggacua ccagcuguac     2940

aagauccggg agaucaacaa cuaccaccac gcccacgacg ccuacaucaa cgccgugguc     3000

gcgacggccc uccugaagaa guacccgcag cuggcccccg aguucgugua cggggaguac     3060

cccaccuaca acagcuacaa cucccgcaag agcgccaccg agaaggugcu guucuacucc     3120

aacaucauga acuucuucaa gcggaucguc gugcacagca agaccggcga ggugcgcauc     3180

cgccccguca ucgaggugaa caaggagacg ggggagaucg ugugggacaa gaaguccgac     3240

uuccggaccg uccgcaaggu gcugagcuac ccccagguga acgucgugaa gaagaccgag     3300

gugcagaacc acggccugga ccggggcaag cccaaggggu ucuacaacgc gaaccucucc     3360

ccgcgcccca aggagggcag cgucgagaac cuggugccgg ccaagcaguc cuucgacacc     3420

aagcgguacg ggggcuacgc cgggaucagc aacagcuacg ccgugcuggu caacggcauc     3480

aucgagaagg gcaagaagaa guccaaggcc gaggugacgg aguuccaggg gaucagcauc     3540

cucgcgcgca aggacuucga caagaacccc aagcaguacc ugcugaaccu cggcuacaag     3600

gacaucaagu ccaucaucaa gcugcccaag uacagccugu ucgagcucga gaacgggucc     3660

cggcgcaugc uggccagcau ccuguccacc aacaacaagc ggggcgagau ccacaagggg     3720

aacgagaugu accucccgga caaguucgug acccugcugu accacgccau gcgcgucaac     3780

cggacccucg agcccggcca caagaaguac guggagacgc accgccacgu guucgacgag     3840

cugcugaucu acauccucga guacaacgag aagaucgucg gcgccaaggc caacgggaag     3900

cggaucgugg aggcguacag cgcccgcaag gacaccgaca accuggagga gcugugcaac     3960

uccuucauca accuccugaa ccugaccgcc cucggcagcg ccguggacuu cgaguuccug     4020

gggaccaaga ucccccggua ccgcgacuac acgcccucca gccugcucaa ggccacccug     4080

auccaccagu ccgucaccgg ccuguacgag acccggaucg accucagcaa gcucgggggc     4140

gacuga                                                                4146


<210>  2050
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2050
augaacuacg ugaaggagua ccacaucggc cuggacaucg ggaccagcuc caucggcugg       60

gccgucaccg acagccaguu caagcucaug cgcaucaagg ggaagacggc caucggcgug      120

cggcuguucg aggaggggaa gaccgcggcc gagcgccgga ccuuccgcac cacgcggcgc      180

cggcugaagc gccggaagug gcgccuccac uaccuggacg agaucuucgc gccccaccuc      240

caggaggugg acgagaacuu ccuccggcgc cugaagcagu ccaacaucca cccggaggac      300

cccgccaaga accaggccuu caucggcaag cugcuguucc ccgaccugcu gaagaagaac      360

gagcggggcu accccacccu caucaagaug cgcgacgagc ugccggucga gcagcgggcc      420

cacuaccccg ugaccaacau cuacaagcug cgcgaggcca ugaucaacga ggaccggcag      480

uucgaccucc gcgaggugua ccuggccguc caccacaucg ugaaguaccg ggggcacuuc      540

cugaacaacg ccagcgugga caaguucaag gucggccgca ucgacuucga caaguccuuc      600

aacgugcuca acgaggcgua cgaggagcug cagaacgggg agggcagcuu caccaucgag      660

cccuccaagg uggagaagau cgggcagcug cuccuggaca cgaagaugcg gaagcuggac      720

cgccagaagg ccgucgccaa guuccucgag gugaaggugg ccgacaagga ggagaccaag      780

cgcaacaagc agaucgccac cgcgaucagc aagcuggucc ugggcuacaa ggccgacuuc      840

gccaccgugg ccauggccaa cggcaacgag uggaagaucg accucuccag cgagacgucc      900

gaggacgaga ucgagaaguu ccgggaggag cugagcgacg cgcagaacga cauccugacc      960

gagaucaccu cccucuucag ccagaucaug cugaacgaga ucgugcccaa cgggaugucc     1020

aucagcgagu ccaugaugga ccgcuacugg acccacgagc ggcagcuggc cgaggucaag     1080

gaguaccucg ccacgcagcc ggccagcgcc cgcaaggagu ucgaccaggu guacaacaag     1140

uacaucggcc aggcgcccaa ggagaagggg uucgaccugg agaagggccu gaagaagauc     1200

cucuccaaga aggagaacug gaaggagauc gacgagcugc ugaaggccgg cgacuuccuc     1260

cccaagcagc ggaccagcgc caacgugguc aucccccacc agaugcacca gcaggagcug     1320

gaccgcauca ucgagaagca ggccaaguac uacccguggc uggccaccga gaaccccgcg     1380

accggggagc gggaccgcca ccaggccaag uacgagcucg accagcuggu gagcuuccgg     1440

auccccuacu acgugggccc ccuggucacg ccggaggugc agaaggccac cuccggggcc     1500

aaguucgcgu gggccaagcg caaggaggac ggcgagauca cccccuggaa ccucugggac     1560

aagaucgacc gggccgagag cgccgaggcc uucaucaagc gcaugaccgu caaggacacg     1620

uaccugcuga acgaggacgu gcuccccgcg aacucccugc uguaccagaa guacaacgug     1680

cugaacgagc ucaacaacgu ccgggugaac gggcgccggc ugagcguggg caucaagcag     1740

gacaucuacu ccgagcuguu caagaagaag aagaccguca aggccagcga cguggccucc     1800

cucgugaugg ccaagacccg cggcgucaac aagcccagcg uggaggggcu guccgacccg     1860

aagaaguuca acagcaaccu ggccaccuac cucgaccuga aguccaucgu gggcgacaag     1920

gucgacgaca acugcuacca gacggaccug gagaacauca ucgaguggcg gagcguguuc     1980

gaggacgggg agaucuucgc ggacaagcuc accgaggugg aguggcugac cgacgagcag     2040

cgcuccgccc uggucaagaa gcgguacaag ggcugggggc gccucagcaa gaagcugcug     2100

accggcaucg uggacgagaa cggccagcgc aucaucgacc ucauguggaa cacggaccag     2160

aacuucaugc agaucgugaa ccagcccguc uucaaggagc agaucgacca gcugaaccag     2220

aacgccauca ccaacgacgg gaugacccug cgggagcgcg uggaguccgu gcucgacgac     2280

gccuacacca gcccccagaa caagaaggcc aucuggcagg ucgugcgggu ggucgaggac     2340

aucgugaagg cggugggcaa cgcccccaag uccaucagca ucgaguucgc ccgcaacgag     2400

gggaacaagg gcgagaucac gcggucccgc cggacccagc ugcagaagcu guucgaggac     2460

caggcccacg agcucgucaa ggacaccagc cugaccgagg agcuggagaa ggcgcucgac     2520

cugagcgacc gcuacuaccu guacuucacc cagggcggga aggacaugua cacgggcgac     2580

ccgaucaacu ucgacgagau cuccaccaag uacgacaucg accacauccu cccccagagc     2640

uucgugaagg acgacucccu ggacaaccgg gugcugguca gccgcgccga gaacaacaag     2700

aaguccgacc gggugcccgc caagcucuac gccgccaaga ugaagcccua cuggaaccag     2760

cugcugaagc aggggcucau cacccagcgc aaguucgaga accugaccau ggacguggac     2820

cagaacauca aguaccggag ccugggcuuc gucaagcgcc agcucgugga gacgcgccag     2880

gugaucaagc ugaccgcgaa cauccugggg uccauguacc aggaggccgg caccgacauc     2940

aucgagaccc gggccggccu cacgaagcag cugcgggagg aguucgaccu gccgaagguc     3000

cgcgagguga acgacuacca ccacgccgug gacgccuacc ucaccaccuu cgcggggcag     3060

uaccugaacc ggcgcuaccc caagcugcgg agcuucuucg ucuacggcga guacaugaag     3120

uucaagcgcg gguccgaccu caagcugcgc aacuucaacu ucuuccacga gcugauggag     3180

ggcgacaaga gccaggggaa ggugguggac cagcagaccg gcgagcucau cacgacccgg     3240

gacgaggucg ccaaguccuu cgaccgccug cugaacauga aguacaugcu cgugagcaag     3300

gaggugcacg accgguccga ccagcuguac ggcgccacca ucgucaccgc caaggagagc     3360

gggaagcuga cgucccccau cgagaucaag aagaaccgcc cgguggaccu cuacggcgcc     3420

uacaccaacg ggaccagcgc guucaugacc aucaucaagu ucacgggcaa caagcccaag     3480

uacaagguga ucgggguccc caccaccucc gccgugggcc ugaagcgggu gggcaagccc     3540

gggagcgaga gcuacaacca ggagcugcac cgcaucauca aguccaaccc gaaggucaag     3600

aaggacuucg agaucguggu cccccacgug agcuacuccc agcucaucgu ggacggcgac     3660

ugcaaguuca cccuggccag cgacacguac cagcaccccg ccacccagcu gguccucucc     3720

aaggagagca uggagaucau cgcggacgac uucaaguucg ugaaggagaa ccccgccacc     3780

gccgacgagc agcuggugcg ggucuucgac gagaucguga accagaugaa ccacuacuuc     3840

accaucuucg accagcgcuc caaccggcag aaggugacga aggcccgcga caaguucguc     3900

agccugccga ccgaguccga guacgagggg gccaagaaga cccagguggg caagaccgag     3960

gugaucacga accuccugau ggggcugcac gcgaacgccg cccagggcga ccucaagguc     4020

cugggccuga gcaccuucgg guucuuccag uccagcggcg ggcucaaccu guccgaggac     4080

gccaugaucg uguaccagag ccccaccggc cuguucgagc ggcgcaucug ccucaaggac     4140

aucuga                                                                4146


<210>  2051
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2051
augaagcaga uccugaagaa ggugcagggc uacaacaucg ggcucgacau cggcaccaac       60

agcgucgggu ggguggugac cgacgagaac gccaaccugc ugcaguuccg caagaagaac      120

auguggggcg cccgccucuu cgagaagggg gacacggcgc agggccggcg gcugucccgc      180

agcacccggc gccgguacga ccgccggcgc cagcggaucc acuaccugca ggagaugcuc      240

gccaccaugg uccugcccau cgacgagucc uucuucaucc gccugaacga ggccuuccuc      300

uggaaggagg acaagagcgu gcagcgggac uacauccugu ucgccgacaa gcaguucacc      360

gaccgcgacu ucuacaagca guucaagacg aucuaccacc uccggaaguu ccuccuggac      420

accaaggaga aggccgaccc gcgcuucauc uaccuggcgc uccaccacau gaucaaguac      480

cggggcaacu uccuguacca ggggcagucc uucgacgugg ucgagaacgu gcaggagagc      540

uuccgcgugc ugcucgagga gauguacgag cagcugggca aggaguggga guacggggag      600

cacuucgacg acauccagcg gauccuggag aacaccacca ucggcaagaa guccaagcag      660

gagcagaucg ccgccauccu cacggagcag uacgggaaca agaagcuggc caacgagguc      720

acccgcgcgu gccugggcua cagcuucgac cucaccgugg ugauggagaa cgagaaccug      780

cuggacgagu ccggcaagaa gaucaagauc agcuucuccg acagcaagua cgaggagcag      840

gagcucgagc ugcagaaccg gcuggaggag aaguucgccg ucaucgaggc ccucaagcgc      900

aucuacaacu ggcuggugcu gaaggacacc cuccgcggga agaaguacau cuccgaggcc      960

auggugaaca aguacgaggc ccaccacgag gaccugguca agcugaagcg ccucuuccgg     1020

acguaccuga gcaccgagga guacuccugc uucuuccgga aggagaagca gaaggacaag     1080

uacaucagca acuacgugaa cuaccgcaag ggcgcggagc ggugcuccaa ggagaagcug     1140

uacaaggcca uccacgagcu guucgacacc guggccaagg acgacgucgu guaccaggag     1200

aucguggaga agauggacca gggggaguuc cugaccaagc agaacgaggu cagcaacgcc     1260

uacauccccu accagugcaa cgagguggag augaagcgca uccugaagca gcagggcgag     1320

uucuaccccg agcucaagga gaacgccgac aagauccugc agcugcuccu guuccggauc     1380

cccuacuacg ugggcccgcu ggucgacgag aagcagucca aguucgcgug gaugaagcgc     1440

cggacgggga uggagcaggu caagaucuac cccuggaacu ucgagcaggu cguggacauc     1500

gaccagaccg ccgaggccuu caucacccgc augaccaacu acugcacgua ccuccccgag     1560

gagaaggugc ugcccaagca gagccugcug uacagcgagu acgagcuccu gcaggagcug     1620

aacaagauca agaucaacgg caaguucucc cucgagaccc ggaacaagau caucgagcag     1680

cuguucaaga agcagaagaa ggugaccgag aagcagcuga agaaguggcu cgugcgcgag     1740

caguacugca ccacgaaggu gaccgagaag auggucaagc uggacgagaa cgccuacuac     1800

aucaccgggu uccagaagga gaacgaguuc gccagcuccc ugaccagcua caucgacuuc     1860

acgaagaucc ucggcgagau caccgagcgg aaccgcaaga ugaucgagga gaucaucuac     1920

uggcugaccg uguucgagga gaaguccauc gugaagcgga agauccagaa gaaguacggg     1980

aacgagauca acgacaagca gcuggcgcag auccucaagc ugaacuacag cggcuggggc     2040

cgcuucuccg agaaguuccu gaacgggauc aagggcuucg accggcaggg gcagaagaag     2100

accaucaugg agcuccugcg cgaggacagc cggcugcuca acaucaugca gaucaucaac     2160

aacgaggaga ugggcuucug caagaucauc gaggccaacc agaagaagcu guacgggaac     2220

gagaaccugc ucgacgucgu gcaggagcug cacggcuccc cggccaucaa gcgcggcauc     2280

uggcagagcc ugaagaucgu ggaggagauc aucuccauca ugggcggggu cccccagaac     2340

aucuucaucg aguucgcccg cgaggacgac gaccgggugc gcacgaacag ccgggugaag     2400

cagaucgagc gccucuacaa gaaguucaag gaggagguca acgacuucaa cgagcacgcc     2460

gcgaacgagc ugaaggagaa gcgguaccag aaccgcaucg acgaggagaa gguguuccug     2520

uacuucaugc agaacgggaa gucccucuac agcggcguga cccuggacau cgaccggcug     2580

aacgaguacg aggucgacca caucaucccc cgcacccucg ugaaggacga cucccuggag     2640

aacaaggccc uggugcucaa gggggagaac cagcggaagg gcgagggccu gaucccccac     2700

gaguggcaga accgccagaa ggaggucugg aagaagcugc ucagcugcgg gcugaugggc     2760

aagaagaagu acgccaaccu gugcaaggag uccuucggga acgagaagga gcagaagcac     2820

uucaucgccc ggcagaucgu ggagacccgc cagaucauca agcaggucgc gaacauccuc     2880

gcccaguacu accagggcgu gaacguggcc gccgugaagg ccagccuggg cuccgagcug     2940

cgggagaacu acacgaucga gaagaaggac gacaacgggu ucuucaugga gaacaagagc     3000

uacaccaugc ucaagagccg caagaucaac gacuuccacc acgcgcagga cgccuaccug     3060

gccugcgccg ucggccugua caucaaggcc gcguucccga aguacgagaa ggagaucgac     3120

uacgaggggu accggaagua cuaccagaag aagaaccagc agaaggacgg cgugcgcaac     3180

gagaagcggc aguucuucuc cuacgugaug agccgcuucu ccgagcagau cgucgacaag     3240

gagaccgggg agauccucug ggacggcacc cagaagcugg ccuacaucaa gaagaucuuc     3300

ggcuaccggg acugccugau caccaagaag cucgaggaga acacggggga guucuacaag     3360

cagaccauca acgccaagcc cgagaagggc aaggagaaca acaccaagcu gcugcccauc     3420

aagaaggggc ucagcgugga gaaguacggc ggguaccagg gcggcgaggc cgccuacucc     3480

ugccuggugc gcuaccagca ggggcggaag accaagcuga gccucgagaa gaucuccguc     3540

aucaucgcga agaagaucgc cgagaaggag gugagccugg accgcuaccu gaaggagaag     3600

cucggcgugg aggagaucgu cauccugcgc aagaagguga ugaagaacca gcugaucgag     3660

gacuccgacg ggaaccgguu cuaccucacg gccgccaccg agguggucaa cgcgaagcag     3720

uucauccugg acggcaagca ccagcaccuc cugcgcacca ucgccaucau ggagaagggg     3780

gaguacuacg gcuaccggcu ggacgagcug gagaacagca uggacgagcu cuacgccuac     3840

uaccgcauga agauccagca gcaguacccc gccuacgagu ccaucgugaa gaagaucgug     3900

gagagccacg ucuacgaguc ccugcacgug gaggagaaga ucaaggccgu gcaggagaag     3960

ggcaaguucc ugauccagcu ccugcggcug accgaggcga acgccgagug caucgaccuc     4020

aagacgauca gcgacgggcg ccuggccgac cggaucggcc gcaagaacca cuacaccauc     4080

aaggagggga ucaaguucau cgaccagucc aucaccggcc uguacgagcg guacaugacc     4140

gucuga                                                                4146


<210>  2052
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2052
auggacuacg ugaaggagua ccacaucggc cuggacaucg ggaccagcuc caucggcugg       60

gccgucaccg acagccaguu caagcucaug cgcaucaagg ggaagacggc caucggcgug      120

cggcuguucg aggaggggaa gaccgcggcc gagcgccgga ccuuccgcac cacgcggcgc      180

cggcugaagc gccggaagug gcgccuccac uaccuggaca agaucuucgc gccccaccuc      240

caggaggugg acgagaacuu ccuccggcgc cugaagcagu ccaacaucca cccggaggac      300

cccgccaaga accaggccuu caucggcaag cuccucuucc ccgaccugcu gaagaagaac      360

gagcggggcu accccacccu caucaagaug cgcgacgagc ugccggucga gcagcgggcc      420

cacuaccccg ugaccaacau cuacaagcug cgcgaggcca ugaucaacga ggaccggcag      480

uucgaccucc gcgaggugua ccuggccguc caccacaucg ugaaguaccg ggggcacuuc      540

cugaacaacg ccagcgugga caaguucaag gucggccgca ucgacuucga caaguccuuc      600

aacgugcuca acgaggcgua cgaggagcug cagaacgggg agggcagcuu caccaucgag      660

cccuccaagg uggagaagau cgggcagcug cuccuggaca cgaagaugcg gaagcuggac      720

cgccagaagg ccgucgccaa gcuccuggag gugaaggugg ccgacaagga ggagaccaag      780

cgcaacaagc agaucgccac cgcgaugagc aagcuggucc ucggcuacaa ggccgacuuc      840

gccaccgugg ccauggccaa cggcaacgag uggaagaucg accuguccag cgagacgucc      900

gaggacgaga ucgagaaguu ccgggaggag cugagcgacg cgcagaacga cauccucacc      960

gagaucaccu cccuguucag ccagaucaug cugaacgcca ucgugcccaa cgggaugucc     1020

aucagcgagu ccaugaugga ccgcuacugg acccacgagc ggcagcucgc cgaggucaag     1080

gaguaccugg ccacgcagcc ggccagcgcg cgcaaggagu ucgaccaggu guacaacaag     1140

uacaucggcc aggugcccaa ggagaagggg uucgaccugg agaagggccu caagaagauc     1200

cuguccaaga aggagaacug gaaggagauc gacgagcugc ucaaggccgg cgacuuccug     1260

cccaagcagc ggaccagcgc caacgggguc aucccccacc agaugcacca gcaggagcug     1320

gaccgcauca ucgagaagca ggccaaguac uacccguggc ucgccaccga gaaccccgcg     1380

accggcgagc gggaccgcca ccaggccaag uacgagcugg accagcuggu gagcuuccgg     1440

auccccuacu acguggggcc ccucgucacg ccggaggugc agaaggccac cuccggcgcc     1500

aaguucgcgu gggccaagcg caaggaggac ggggagauca cccccuggaa ccugugggac     1560

aagaucgacc gggccgagag cgccgaggcc uucaucaagc gcaugaccgu caaggacacg     1620

uaccugcuca acgaggacgu gcugcccgcg aacucccugc uguaccagaa guacaacgug     1680

cucaacgagc ugaacaacgu ccgggugaac ggccgccggc ugagcguggg caucaagcag     1740

gacaucuaca ccgagcuguu caagaagaag aagaccguca aggccgggga cguggccucc     1800

cuggugaugg ccaagacccg cggcgucaac aagcccagcg uggaggggcu guccgacccg     1860

aagaaguuca acagcaaccu cgccacguac cuggaccuga aguccaucau gggcgacaag     1920

guggacgaca accgguacca gauggaccuc gagaacauca ucgaguggcg cagcgucuuc     1980

gaggacgggg agaucuucgc ggacaagcug accgaggugg aguggcugac cgacgagcag     2040

cgguccgccc ucgugaagaa gcgcuacaag ggcuggggcc gccugagcaa gaagcugcuc     2100

accgggaucg ucgacgagaa cggccagcgg aucaucgacc ugauguggaa cacggaccag     2160

aacuucaugc agaucgugaa ccagcccgug uucaaggagc agaucgacca gcugaaccag     2220

aaggccauca ccaacgacgg gaugacccuc cgcgagcggg ucgaguccgu gcuggacgac     2280

gccuacacca gcccccagaa caagaaggcc aucuggcagg ucgugcgcgu gguggaggac     2340

aucgucaagg cggugggcaa cgcccccaag uccaucagca ucgaguucgc ccggaacgag     2400

gggaacaagg gcgagaucac gcgcucccgg cgcacccagc ugcagaagcu guucgaggac     2460

caggcccacg agcuggugaa ggacaccagc cugaccgagg agcucgagaa ggcgccggac     2520

cuguccgacc gguacuacuu cuacuucacc cagggcggga aggacaugua cacgggcgac     2580

cccaucaacu ucgacgagau cagcaccaag uacgacaucg accacauccu gccccagagc     2640

uucgucaagg acgacucccu cgacaaccgc gugcugguga gccgggccga gaacaacaag     2700

aaguccgacc gcguccccgc caagcuguac gccgccaaga ugaagccgua cuggaaccag     2760

cuccugaagc aggggcugau cacccagcgg aaguucgaga accucaccau ggacguggac     2820

cagaacauca aguaccgcag ccugggcuuc gugaagcggc agcuggucga gacgcgccag     2880

gugaucaagc ucaccgcgaa cauccugggc uccauguacc aggaggccgg gaccgacauc     2940

aucgagaccc gggccggccu gacgaagcag cuccgcgagg aguucgaccu gcccaaggug     3000

cgggagguca acgacuacca ccacgccgug gacgccuacc ugaccaccuu cgcggggcag     3060

uaccucaacc gccgguaccc caagcugcgc agcuucuucg uguacggcga guacaugaag     3120

uucaagcacg gguccgaccu gaagcuccgc aacuucaacu ucuuccacga gcugauggag     3180

ggcgacaaga gccagggcaa ggucguggac cagcagaccg gggagcugau cacgacccgg     3240

gacgaggugg ccaaguccuu cgaccgccuc cugaacauga aguacaugcu ggucagcaag     3300

gaggugcacg accgguccga ccagcucuac ggcgccacca ucgugaccgc caaggagagc     3360

gggaagcuga cguccccgau cgagaucaag aagaaccgcc ccgucgaccu guacggcgcc     3420

uacaccaacg ggaccagcgc guucaugacc aucaucaagu ucacgggcaa caagcccaag     3480

uacaagguga ucggcgugcc caccaccucc gccgucgggc ucaagcgggu gggcaagccg     3540

gggagcgagu ccuacaacca ggagcugcac cgcaucauca agagcaaccc caaggugaag     3600

aaggacuucg agaucgucgu gccccacguc agcuacuccc agcugaucgu ggacggcgac     3660

ugcaaguuca cccucgccag cgacacguac cagcaccccg ccacccagcu ggugcugucc     3720

aaggagagca uggagaucau cgcggacgac uucaaguucg ucaaggagaa cccggccacc     3780

gccgacgagc agcucgugcg gguguucgac gagaucguca accagaugaa ccacuacuuc     3840

accaucuucg accagcgcuc caaccggcag aaggugacga aggcccgcga caaguucgug     3900

agccugccca ccgaguccga guacgagggg gccaagaaga cccaggucgg caagaccgag     3960

gugaucacga accugcucau gggccugcac gcgaacgccg cccaggggga ccugaaggug     4020

cucggccuga gcaccuucgg guucuuccag uccagcggcg ggcugaaccu cuccgaggac     4080

gccaugaucg ucuaccagag ccccaccggc cuguucgagc ggcgcaucug ccugaaggac     4140

aucuga                                                                4146


<210>  2053
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2053
augaagaagu ccuacgccau cggcaucgac aucgggacca acagcguggg ccacugcguc       60

auguacaccg agaacuacaa ggugcccagc aagaagauga agguguucgg gaacacggag      120

aagcgcuaca ucaagaagaa ccugcucggc acccugcugu ucgacgaggg gaacaccgcc      180

gagaaccggc gccucaagcg gaccgcgcgc cggcgcuaca cgcggcgccg gaaccgcauc      240

cuguaccugc aggagaucuu cgccgaggag aucaacaaga ucgacgacuc cuucuuccag      300

cggcucgacg acagcuuccu gaucgucgag gacaagcagg gcuccaagca cccgaucuuc      360

ggcacccugc aggaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacagcuc ccagaaggcc gacauccggc ugaucuaccu cgcccuggcg      480

cacaucauca aguaccgcgg gcacuuccug uucgagggcg accucaagag cgagaacaag      540

gacgugcagc accuguucaa cgacuucgug gagauguucg acaagaccgu cgaggggucc      600

uaccugagcg agaaccuccc caacguggcc gacgugcugg ucgagaaggu guccaagagc      660

cggcgccugg agaacauccu ccacuacuuc cccaacgaga agaagaacgg ccuguucggg      720

aacuuccugg cccucgcccu gggccugcag ccgaacuuca agacgaacuu cgagcucgcg      780

gaggacgcca agauccaguu cuccaaggag accuacgagg aggaccugga ggagcugcuc      840

ggcaagaucg gggacgacua cgccgaccug uucaucgcca ccaagagccu guacgacggc      900

auccuccugg ccgggauccu guccaccacg gacagcacca ccaaggcgcc ccucuccagc      960

uccaugguga accgguacga ggagcaccag aaggaccugg cccugcucaa ggacuucauc     1020

cgccagaacc ugagcgacag cuacaaggag uucuucaacg acacccugaa ggacggcuac     1080

gccggcuaca ucgaggggaa gacgacccag gagaacuucu accgguucau caagaaggcc     1140

aucgagaaga ucgagggcuc caacuacuuc aucgacaaga ucgaccgcga ggacuuccuc     1200

cggaagcagc gcaccuucga caacgggagc aucccccacc agauccaccu ccaggagaug     1260

cacgccauca uccgccggca ggcggaguuc uaccccuucc uggucgagaa ccaggacaag     1320

aucgagaaga uccucaccuu ccgcaucccg uacuacgugg gcccccuggc ccgggggaag     1380

uccgaguucg ccuggcugaa gcgcaagagc gacgagaaga uccggcccug gaacuucgac     1440

gagauggugg acaaggagac guccgccgag aacuucauca cccgcaugac caacuacgac     1500

caguaccucc ccgaccagaa gguccugccg aagcacagcc ugcuguacga gaaguucgcc     1560

guguacaacg agcucaccaa ggugcgguac gucacggagc agggcaaguc cuucuucuuc     1620

gacgcgaaca ugaagcagga gaucuucgac ggcguguuca agguguaccg caaggucacc     1680

aaggagaagc ugauggacuu ccuggggaag gaguucgacg aguuccggau cguggaccuc     1740

augggccugg acaaggacaa caagagcuuc aacgccuccc uggggaccua ccacgaccuc     1800

aagaagaucg ugagcaagga ccugcuggac aaccccgaga acgaggacau ccucgagaac     1860

gucgugcuga cccugacgcu guucgaggac cgcgagauga uccggaagcg ccuggagaag     1920

uacaaggacg uccugaccga ggagcagcgg aagaagcucg agcgccggca cuacaccggc     1980

ugggggcgcc uguccgccaa gcugaucaac ggcauccgga acaagaccac gcgcaagacc     2040

auccucgacu accugaucga cgacggcacc agcaaccgga acuucaugca gcugauccac     2100

gacgacaccc ucuccuucgu ggacgagauc cgccuggccc aggggagcgg cgaggccgag     2160

gacuaccgca cggaggugca gaaccuggcg gggucccccg ccaucaagaa gggcauccuc     2220

cagagccuga agaucgucga cgagcugauc ggggugaugg gcuacaaccc cgagcacgug     2280

gucguggaga uggcccggga gaaccaguuc accaaccagg gccgccgguc cagccagcag     2340

cgcuacaaga agaucgagaa cgccaucaag aaccucgacu ccaaccugaa cagcaagauc     2400

cugaaggagu acccgaccaa caaccaggcg cuccagaacg accggcuguu ccuguacuac     2460

cuccagaacg ggaaggacau guacaccgac gaggagcugg acaucgacca gcugagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacucccu cgacaacaag     2580

gugcugacca agagcgccaa gaaccgcggc aaguccgacg acguccccag ccuggagauc     2640

gugcacaaga agaagaacuu cuggaagcag cuccuggacu cccagcugau cagccagcgg     2700

aaguucgaca accucacgaa ggccgagcgc gggggccuga ccaacgagga caaggcccgg     2760

uucauccagc gccagcuggu ggagacccgg cagaucacca agcacgucgc gcgcauccuc     2820

gacacgcggu ucaacaccaa gcuggacgac gccggcaacc gcauccggga ccccaaggug     2880

aacaucauca cccugaaguc cagccucgug ucccaguucc gcaaggacua ccagcuguac     2940

aagguccggg agaucaacaa cuaccaccac gcccacgacg ccuaccugaa cgccguggug     3000

gcgaccgccc uccugaagaa guacccgcag cuggcccccg aguucgucua cggggacuac     3060

cccaaguaca acagcuacaa gucccgcaag agcgccacgg agcgggugcu guucuacucc     3120

aacaucauga acuucuucaa gcgcgugguc aagagcucca agaccggcac cgugaagauc     3180

cggcccauca ucgaggugaa caaggagacc ggggagaucg ucugggacaa gaaguccgac     3240

uuccgcacgg ugcgcaaggu gcugagcuac ccgcagguca acguggugaa gaagaccgag     3300

guccagaacc acggccugga ccgggggaag cccaagggcu ucuacaacgc caaccucagc     3360

ccccgcccca aggagggcag cguggagaac cuggugccgg cgaagcaguc cuucgacacc     3420

aagcgguacg ggggcuacgc cgggaucagc aacuccuacg ccguccuggu gaacggcauc     3480

aucgagaagg ggaagaagaa guccaaggcc gaggugaccg aguuccaggg caucagcauc     3540

cucgcgcgca aggacuucga gaagaacccc aagcaguacc ugcugaaccu cggcuacaag     3600

gacaucaaga gcaucaucaa gcugcccaag uacucccugu ucgagcucga gaacgggagc     3660

cggcgcaugc uggccuccau ccugagcacg aacaacaagc ggggcgagau ccacaagggg     3720

aacgagaugu accucccgga caaguucguc acccugcugu accacgccau gcgcgugaac     3780

cggacccucg agcccggcca caagaaguac guggagaccc accgccacgu cuucgacgag     3840

cugcugacgu acauccucga guacaacgag aagaucgugg gggccaaggc caacggcaag     3900

cggaucgugg aggcguacuc cgcccgcaag gacaccgaca accuggagga gcugugcaac     3960

agcuucauca accuccugaa ccugaccgcc cucggcuccg ccgucgacuu cgaguuccug     4020

gggaccaaga ucccccggua ccgcgacuac acgcccagcu cccugcucaa ggccacccug     4080

auccaccaga gcgugaccgg ccuguacgag acccggaucg accucuccaa gcucgggggc     4140

gacuga                                                                4146


<210>  2054
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2054
augaagaaga ucaucggccu ggaccucggg accaccagca ucggcugggc cuacgugcac       60

gaggccgagu ccgcgcagga gcgcagcgag aucguccggc ugggggcccg cguggugccc      120

cugacgaccg acgagcagga cgaguucaag aagggcaagu ccaucaccac caacgccgcc      180

cggcucagca agcgcucccu gcggcgcaac gccgaccggu uccagcugcg ccgggacgag      240

cucgcggggg ucaugaagga ggccggcugg aucgacgaga cgaccgugcu ggccgagcag      300

ggcccgcgca gcaccuucga gacccugcgg cuccgcgcca aggcggccuc cgaggaggug      360

acgcuggccg aguucgcccg gauccugcuc cagaucaacc gcaagcgggg guacaagagc      420

aaccgcaaga ccgacggcga cgaggagggg gcccugaucg acggcaugga cgucuccaag      480

cagcugaacg cgagcgggca gacccccggc gagugggugc ucgcccggau gcaggagggc      540

aacgccguga uccccgccuu cuacugcucc gaccugagcg aggagcugga ccgcaucugg      600

cggaagcagc aggccuccca ccaggcgugg augaccgagg agcuccacac gguccugcgc      660

cacgccgacg ggcggagcgc cauccgccug auggacaagg ccggcguggu gcccgacucc      720

gucaacguga aggagcgccg gcgcgugaug uacgaguggc gggccgcggc cguccgcgac      780

ugccucccgg ccgggcgggu ggccgccgcg cugaugcagg ucagcaagga gaucggccgc      840

gccgacuccu accugggccg gaucggggac cgcagcaagg agcucuacuu ccaccggcag      900

accgugggcc aguaccugug gcgccugauc ugcgacaacc ccaaccaguc ccucaagaac      960

cugguguucu accggcagga cuaccagcac gaguucgaga cccuguggag cauccagacc     1020

aaguaccacg uggagcucac ggaggaccug cgcaagcggg ucgggcgcau caucuucuuc     1080

cagcggcccc ucaagagcaa gaagggccuc gugaaccgcu gcgagcuggu gcacuacucc     1140

gucugcgaga acagcgggga cgaccugacc gagaaggugg acgucggccc caaggugugc     1200

ccgaagucca gcccccucuu ccaggaguuc aagguguggc agguccugaa caacguggag     1260

gugauccacc ccuccaccca ggaggcccgg cgccuggaga uggaggagaa ggagcuccug     1320

gccagcgagc ugcuguuccg ggaccgcaug aagaagaucg acguccugaa gcuccuguuc     1380

uccaacuggc gcgaccugga ccugaacuac aaggagcucg agggcaaccg gaccauggcg     1440

gccuucuacc gcguguacga gcaggucauc gcccagacgg ggcacggcga cuacgacuuc     1500

gccaagaugc gggccgcgaa ggugaaggag cuggccgagg ggguguucgc cggccugggg     1560

uucgaggugg gccugcucac cuucgacgcc gagcuggaca gccccgccua cgagcagcag     1620

ccgugcuacc gccuguggca ccuccuguac uccuacgagg gcgacaagag cgcgaccggg     1680

aacaagugcc ugcucaagaa gcugugccgg cugaccggca ugaacgccga gcucgucggg     1740

cuguucaugc agauccgcuu cuccggcgac uacgggagcc uguccacgaa ggccaugcgg     1800

cgcauccucc ccuaccugaa ggccggcaac accuacgacg uggccugcga guacgcgggc     1860

uuccggcaca gcgcccgcuc ccugacccgg gaggagcucg cccagaagga guacaaggac     1920

uaccuggacc ugcucccccg caacagccug cggaaccccg uggucgagaa gauccugaac     1980

cagaugaucc acguggugaa cgggcucucc agcaccuacg gcaagccgga cgagauccgc     2040

gucgagaugg cccgggagcu gaagaagucc gcgcgcgagc gggaggagau gacgagcgcc     2100

aucagcaaga ccaccaagga gaacgccgag aucaccgcca uccugcagcg cgacuucggg     2160

cuccgguacg ugucccgcaa cgacauccug cgguacaagc uguacugcga gcuccgcauc     2220

aagggcaacg gcugccgcac gcuguacagc aacaccuaca ucaaccccaa cgagcuguuc     2280

ucccgggagu ucgacaucga gcacaucauc ccccagagcc gccucuucga cgacuccuuc     2340

agcaacaaga cccuggaguc ccggaccgcc aaccuggaga agggggaccg cacggcgugg     2400

gacuacguga acgagaagua cggcgccgag cgggccgacg aguaccgcgc ccaggucgag     2460

gcccucuacc gggacgggac caucagcaag gcgaagcgcg acaagcugcu gaugcccggc     2520

gacaagaucc cggaggacuu caucgaccgg gacauccgca acacccagua caucgccaag     2580

aaggcccucg agaucuucga ggacuucgug cccuccgugg ucgccaccag cgggaacgug     2640

accgcccggc ugcgcgagga cuggggccug guggacguca ugaaggagcu caacuggucc     2700

aaguacgaga gccagggcau ggugcacauc gaguacgacc gggacgggcg ccccauccgg     2760

cgcaucgugg acuggacgaa gcggaacgac caccgccacc acgcgaugga cgcccuggcc     2820

gucgccuuca ccaagcgguc cuacauccag uaccugaacc accucaacgc gcgcaaggag     2880

gccaccagcg uggccuacgc cauccagcac aaggagaugg agcuggacgg cuccaaccgc     2940

uggcgguuca agugccccuu cggggaccgc agcgccuucc ggcgcgaggc gaaggcccag     3000

cuggagcgga uccucguguc caucaagagc aagaacaagg uccugacccc gagcgugaac     3060

cgcacgaaga agcggggcgg guucaacaag aagguggagu ggacgccccg cggcgcccug     3120

cacaacgaga ccgucuacgg ccggauccgc cgguacgacg ugaaggagcu ccgcgugggg     3180

uccagcuuca ccgccgaggu caucggcaag guggccgacu cccgguaccg cgcggcccug     3240

augcaccggc uggccgaguu cgggggcgac cccaagaagg ccuucaccgg gaagaacagc     3300

cucgagaaga acccccugua caccgacgag gcccacaccg cgugcgugcc guccagcguc     3360

aagguggugu ccuucgucga gaucggcacc auccgcaagg gcguggaccc caaccugaac     3420

guggacaagg ucguggacac gcacaucaag aacauccucc uggcccggcu gagcgccuac     3480

gggggcgacg ccaagaaggc cuucuccaac cucgacgcgg accccaucug gcugaacaag     3540

gagaagggga ucgccaucaa gcgcgugacc aucagcgacg acuccgaggu ccggccgauc     3600

cacgugaagc gcgacaugcg gggccgccug gucugcgacg agcacgggcg ccccgugccc     3660

agcgacuacg ugcagcuccg gaacaaccac cacgucgcca ucuuccgcga cgccaacggc     3720

gagcugaagg agcggguggu gaccuucuuc gaggcggucg agcgcgugcg gcagcugcgc     3780

uccgagggcc gggccgugga ggucaucgac aagaacuaca agugcgagga gggguggacc     3840

uuccucuuca gcaugaagau caacgaguac uucguguucc ccgacgccgc cuccggcuuc     3900

gacccgcgcg agaucgaccu gacggacccc gccaacuacg cgcugaucag cccccaccuc     3960

uuccgggugc agaagcuguc cagcaaggac uacuacuucc gccaccaccu ggagaccacc     4020

gucaacgacg ugaagcagcu ccgggacauc accuggaagc gcaucuccag ccugcaggac     4080

cuggccggga cggugaaggu ccgggugaac cacaucggcc agaucguggc cgucggcgag     4140

uacuga                                                                4146


<210>  2055
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2055
auggugaaga agaaguacgc caucggcauc gacaucggga ccaacagcgu cggcuggucc       60

aucgugaccg acgacuacaa ggugcccagc aagaagauga aggucuucgg gaacacggag      120

aagcgcuaca ucaagaagaa ccugcucggc acccugcugu ucgacgaggg gaacaccgcc      180

gagaaccggc gccucaagcg gaccgcgcgc cggcgcuaca cgcggcgccg gaaccgcauc      240

cuguaccugc aggagaucuu cgccgaggag aucaacaaga ucgacgacuc cuucuuccag      300

cggcucgacg acagcuuccu gaucguggag gacaagcagg gcuccaagca cccgaucuuc      360

ggcacccugc aggaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacagcuc ccagaaggcc gacauccggc ugaucuaccu cgcccuggcg      480

cacaucauca aguaccgcgg gcacuuccug uucgagggcg accucaagag cgagaacaag      540

gacgugcagc accuguucaa cgacuucguc gagauguucg acaagaccgu ggaggggucc      600

uaccugagcg agaaccuccc caacguggcc gacguccugg uggagaaggu guccaagagc      660

cggcgccugg agaacauccu ccacuacuuc cccaacgaga agaagaacgg ccuguucggg      720

aacuuccugg cccucgcccu gggccugcag ccgaacuuca agacgaacuu cgagcucgcg      780

gaggacgcca agauccaguu cuccaaggag accuacgagg aggaccugga ggagcugcuc      840

ggcaagaucg gggacgacua cgccgaccug uucaucgcca ccaagagccu guacgacggc      900

auccuccugg ccgggauccu guccaccacg gacagcacca ccaaggcgcc ccucuccagc      960

uccaugguca accgguacga ggagcaccag aaggaccugg cccugcucaa gaacuucauc     1020

caccagaacc ugagcgacag cuacaaggag guguucaacg acaagcugaa ggacggcuac     1080

gccggcuaca ucgaggggaa gaccacgcag gagaacuucu accgcuucau caagaaggcc     1140

aucgagaaga ucgagggcuc caacuacuuc aucgacaaga ucgaccggga ggacuuccuc     1200

cgcaagcagc ggaccuucga caacgggagc aucccccacc agauccaccu ccaggagaug     1260

cacgccauca uccgccgcca ggcggaguuc uaccccuucc ugguggagaa ccaggacaag     1320

aucgagaaga uccucaccuu ccggaucccg uacuacgucg gcccccuggc ccgcgggaag     1380

uccgaguucg ccuggcugaa ccggaagucc gacgagaaga uccgccccug gaacuucgac     1440

gagauggugg acaaggagac cuccgccgag aacuucauca cgcggaugac caacuacgac     1500

caguaccucc ccgaccagaa ggugcugccg aagcacagcc ugcuguacga gaaguucgcc     1560

gucuacaacg agcucaccaa ggugcgcuac gugaccgagc agggcaaguc cuucuucuuc     1620

gacgcgaaca ugaagcagga gaucuucgac cggacguuca aggucuaccg caaggugacc     1680

aaggagaagc ugauggacuu ccugagcaag gaguucgacg aguuccggau cguggaccuc     1740

cugggccugg acaaggacaa caaguccuuc aacgccagcc ucgggaccua ccacgaccug     1800

aagaagaucg ucuccgagga cuuccuggac aacagcgaga acgaggacau ccucgagaac     1860

gugguccuga cccugacgcu guucgaggac cgcgagauga uccggaagcg ccuggagaag     1920

uacaaggacg ugcugaccga ggagcagcgg aagaagcucg agcgccggca cuacaccggc     1980

ugggggcgcc uguccgccaa gcugaucaac ggcauccggg acaaggugac ccgcaagacg     2040

auccucgacu accugaucga cgacgggacc agcaaccgga acuucaugca gcugaucaac     2100

gacgacaccc ucuccuucgu cgacgagauc cgccuggccc agggcagcgg caaggccgag     2160

gacuaccgcg cggaggugca gaaccuggcc gggucccccg ccaucaagaa gggcauccuc     2220

cagagccuga agaucgugga cgagcugauc gaggucaugg gguacgaccc cgagcacauc     2280

gugguggaga uggcccggga gaaccaguuc accaaccagg gccgccggaa cucccagcag     2340

cgcuacaaga agaucgagaa cgcgaucaag aaccucgaca gcaaccugaa cagcaagauc     2400

cugaaggagu accccacgaa caaccaggcc cuccagaacg accggcuguu ccuguacuac     2460

cuccagaacg ggaaggacau guacaccgac gaggagcugg acaucgacca gcugucccag     2520

uacgacaucg accacaucau cccgcaggcc uucaucaagg acgacagccu cgacaacaag     2580

guccugacca aguccgccaa gaaccgcggc aagagcgacg acgugcccuc ccuggagauc     2640

guguacaaga agaagaacuu cuggcagcag cuccuggaca gccagcugau cucccagcgg     2700

aaguucgaca accucaccaa ggccgagcgc ggcgggcuga ccaacgagga caaggcgcgg     2760

uucauccagc gccagcuggu cgagacgcgg cagaucacca agcacguggc ccgcauccuc     2820

gacacccggu ucaacaccaa gcuggacgag gccggcaacc gcauccggga ccccaaggug     2880

aacaucauca uccucaagag caaccucguc ucccaguucc gcaaggacua ccagcuguac     2940

aaggugcggg agaucaacaa cuaccaccac gcccacgacg ccuaccugaa cgcggugguc     3000

gccacggccc uccugaagaa guacccccag cuggccccgg aguucgugua cggggaguac     3060

cccaccuaca acagcuacaa cucccgcaag agcgccaccg agaaggugcu guucuacucc     3120

aacaucauga acuucuucaa gcggaucguc gugcacagca agaccggcga ggugcgcauc     3180

cggcccguca ucgaggugaa caaggagacg ggcgagaucg ugugggacaa gaaguccaac     3240

uuccgcaccg uccgcaaggu gcugagcuac ccccagguga acgucgugaa gaagaccgag     3300

gugcagaacc acgggcugga ccggggcaag ccgaaggggu ucuacaacgc gaaccucagc     3360

ccccgcccca aggagggcag cgucgagaac cuggugcccg ccaagcagag cuucgacacc     3420

aagcgguacg ggggcuacgc cggcaucucc aacagcuacg ccgugcuggu caacgggauc     3480

aucgagaagg gcaagaagaa guccaaggcg gaggugacgg aguuccaggg gaucagcauc     3540

cucgcccgca aggacuucga caagaacccg aagcaguacc ugcugaaccu cggcuacaag     3600

gacaucaagu ccaucaucaa gcugcccaag uacagccugu ucgagcucga gaacgggucc     3660

cggcgcaugc uggccagcau ccuguccacc aacaacaagc ggggcgagau ccacaagggc     3720

aacgagaugu accuccccga caaguucgug acccugcugu accacgccau gcgcgucaac     3780

cggacccucg agccggggca caagaaguac guggaggccc accgccacgu guucgacgag     3840

cugcugacgu acauccucga guacaacgag aagaucgucg gcgcgaaggc caacgggaag     3900

cggaucgugg aggccuacag cgcccgcaag gacaccgaca accuggagga gcugugcaac     3960

uccuucauca accuccugaa ccugaccgcc cucggcagcg cgguggacuu cgaguuccug     4020

gggaccaaga ucccccggua ccgcgacuac acgcccucca gccugcucaa ggccacccug     4080

auccaccagu ccgucaccgg ccuguacgag acccggaucg accucagcaa gcugggcggg     4140

gacuga                                                                4146


<210>  2056
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2056
auggugaaga agaaguacgc caucggcauc gacaucggga ccaacagcgu cggcuggucc       60

guggugaccg acgacuacaa gguccccagc aagaagauga agguguucgg gaacacggag      120

aagcgcuaca ucaagaagaa ccugcucggc acccugcugu ucgacgaggg gaacaccgcc      180

gagaaccggc gccucaagcg gaccgcgcgc cggcgcuaca cgcggcgccg gaaccgcauc      240

cuguaccugc aggagaucuu cgccgaggag aucaacaaga ucgacgacuc cuucuuccag      300

cggcucgacg acagcuuccu gaucguggag gacaagcagg gcuccaagca cccgaucuuc      360

ggcacccugc aggaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacagcuc ccagaaggcc gacauccggc ugaucuaccu cgcccuggcg      480

cacaucauca aguaccgcgg gcacuuccug uucgagggcg accucaagag cgacaacaag      540

gacguccagc accuguucaa cgaguucgug gagauguucg acaagaccgu ggaggggucc      600

uaccugagcg agaacauccc cgacgucgcc gacauccucg uggagaaggu guccaagagc      660

cggcgccugg agaacguccu gcaguacuuc cccaacgaga agaagaacgg ccuccugggg      720

aacuuccugu cccucgcccu gggccugcag ccgaacuuca agacgaacuu cgagcucgcc      780

gaggacgcga agauccaguu cagcaaggag accuacgagg aggaccugga ggagcugcuc      840

ggcaagaucg gggacgacua cgccgaccug uucaucgcca ccaagucccu guacgacggc      900

auccuccugg ccgggauccu gagcaccacg gacuccacca ccaaggcccc gcucagcucc      960

agcaugguga accgguacga ggagcaccag aaggaccugg cgcugcucaa ggacuucauc     1020

cgccagaacc ugagcgacuc cuacaaggag guguucaacg acgcccugaa ggacggcuac     1080

gccggcuaca ucgaggggaa gaccacgcag gaggacuucu acaaguucgu caagaagacc     1140

aucgagaaga ucgagggcag cgacuacuuc aucgacaaga ucgaccggga ggacuuccuc     1200

cgcaagcagc ggaccuucga caacgggucc aucccccacc agauccaccu gaaggagaug     1260

caggccauca ucauccgcca ggccaaguuc uaccccuucc uggaggagaa ccaggacaag     1320

aucgagaaga uccucaccuu ccgcaucccg uacuacgugg gcccccuggc gcgggggaac     1380

agcgaguucg ccuggcugaa ccgcaagucc gacgagaaga uccggcccug gaacuucgac     1440

gagaucgugg acaaggagac gagcgccgag aacuucauca cccgcaugac caacuacgac     1500

caguaccucc ccgaccagaa gguccugccg aagcacuccc ugcuguacga gaaguucgcc     1560

guguacaacg agcucaccaa ggugcgguac gucacggagc agggcaagag cuucuucuuc     1620

gacgccaaca ugaagcagga gaucuucgac ggcguguuca agguguaccg caaggucacc     1680

aaggagaagc ugauggacuu ccuggggaag gaguucgacg aguuccggau cguggaccuc     1740

cugggccugg acaaggacaa caaguccuuc aacgcgagcc ucgggaccua ccacgaccug     1800

aagaagaucg ucuccaagga ccugcucgac aaccccgaga acgaggacau ccuggagaac     1860

guggugcuga cccucacgcu guucgaggac cgcgagauga uccggaagcg ccuggagaag     1920

uacaaggagg uccucaccga ggagcagcgg aagaagcugg agcgccggca cuacaccggc     1980

ugggggcgcc ugagcgccaa gcucaucaac ggcauccggg acaaggugac ccgcaagacg     2040

auccuggacu accugaucga cgacggcacc uccaaccgga acuucaugca gcucaucaac     2100

gacgacaccc ugagcuucgu ggacgagauc cgccuggccc agggguccgg cgaggccgag     2160

gacuaccggg ccgaggucca gaaccucgcg gggagccccg ccaucaagaa gggcauccug     2220

cagucccuga agaucgugga cgagcucauc gaggugaugg gguacgagcc cgagcacauc     2280

gucguggaga uggcccgcga gaaccaguuc accaaccagg gccgccggaa cagccagcag     2340

cgcuacaaga agaucgagaa cgccaucaag aaccuggacu ccaaccugaa cagcaagauc     2400

cucaaggagu acccgacgaa caaccaggcg cugcagaacg accggcuguu ccucuacuac     2460

cugcagaacg gcaaggacau guacaccgac gaggagcugg acaucgacca gcucagccag     2520

uacgacaucg accacaucau cccccaggcc uucaucaagg acgacucccu ggacaacaag     2580

gugcugacca agagcgccaa gaaccgcggg aaguccgacg acguccccag ccucgagauc     2640

guguacaaga agaaguccuu cuggaagcag cugcuggaca gccagcucau cucccagcgg     2700

aaguucgaca accugaccaa ggccgagcgc ggcgggcuga ccaacgagga caaggcccgg     2760

uucauccagc gccagcucgu ggagacgcgg cagaucacca agcacgucgc gcgcaugcug     2820

gacacccggu ucaacaccaa gcuggacgag gccggcaacc gcauccggga ccccaaggug     2880

aacaucauca cgcucaagag caaccuggug ucccaguucc gcaaggacua ccagcuguac     2940

aagguccggg agaucaacaa cuaccaccac gcccacgacg ccuaccucaa cgccguggug     3000

gcgaccgccc ugcugaagaa guacccgcag cucgcccccg aguucgucua cggcgaguac     3060

cccaccuaca acagcuacaa cucccgcaag agcgccaccg agaaggugcu guucuacucc     3120

aacaucauga acuucuucaa gcggaucgug guccacagca agacggggga ggugcgcauc     3180

cggcccguga ucgaggucaa caaggagacc ggcgagaucg ugugggacaa gaaguccgac     3240

uuccgcaccg ugcggaaggu ccugagcuac ccccagguga acguggucaa gaagaccgag     3300

gugcagaacc acgggcucga ccgcggcaag cacaaggggu ucuacaacgc caaccugagc     3360

ccccgcccca aggagggcuc cguggagaac cugguccccg cgaagcagag cuucgacacg     3420

aagcgguacg gcggguacgc cggcaucucc aacagcuacg ccgugcucgu gaacgggauc     3480

aucgagaagg gcaagaagaa guccaaggcc gaggucaccg aguuccaggg gaucagcauc     3540

cuggcgcgca aggacuucga caagaacccg aagcaguacc ugcucaaccu gggcuacaag     3600

gacaucaagu ccaucaucaa gcugcccaag uacagccucu ucgagcugga gaacggcucc     3660

cggcgcaugc uggccagcau ccucuccacc aacaacaagc gcggggagau ccacaagggc     3720

aacgagaugu accugcccga caaguucgug acccugcucu accacgccau gcgggugaac     3780

cggacgcugg agccggggca caagaaguac gucgagaccc accgccacgu guucgacgag     3840

cugcucaucu acauccugga guacaacgag aagaucgugg gcgccaaggc caacgggaag     3900

cggaucgucg aggcguacag cgcccgcaag gacaccgaca accuggagga gcucugcaac     3960

uccuucauca accugcugaa ccucaccgcc cugggcagcg ccguggacuu cgaguuccug     4020

ggcacgaaga ucccccggua ccgcgacuac acccccucca gccuccugaa ggccacccug     4080

auccaccagu ccgugaccgg gcucuacgag acgcggaucg accugagcaa gcugggcggg     4140

gacuga                                                                4146


<210>  2057
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2057
auggugaaga agaaguacgc caucggcauc gacaucggga ccaacagcgu cggcuggucc       60

guggugaccg acgacuacaa gguccccagc aagaagauga agguguucgg gaacacggag      120

aagcgcuaca ucaagaagaa ccugcucggc acccugcugu ucgacgaggg gaacaccgcc      180

gagaaccggc gccucaagcg gaccgcgcgc cggcgcuaca cgcggcgccg gaaccgcauc      240

cuguaccugc aggagaucuu cgccgaggag aucaacaaga ucgacgacuc cuucuuccag      300

cggcucgacg acagcuuccu gaucguggag gacaagcagg gcuccaagca cccgaucuuc      360

ggcacccugc aggaggagaa ggaguaccac aagcaguucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacagcuc ccagaaggcc gacauccggc ugaucuaccu cgcccuggcg      480

cacaucauca aguaccgcgg gcacuuccug uucgagggcg accucaagag cgagaacaag      540

gacguccagc accuguucaa cgacuucgug gagauguucg acaagaccgu ggaggggucc      600

uaccugagcg agaaccuccc caacgucgcc gacgugcugg uggagaaggu cuccaagagc      660

cggcaccugg agaacauccu ccacuacuuc cccaacgaga agaagaacgg ccuguucggg      720

aacuuccugg cccucgcccu gggccugcag ccgaacuuca agacgaacuu cgagcucgcg      780

gaggacgcca agauccaguu cuccaaggag accuacgagg aggaccugga ggagcugcuc      840

ggcaagaucg gggacgacua cgccgaccug uucaucgcca ccaagagccu guacgacggc      900

auccuccugg ccgggauccu guccaccacg aacagcacca ccaaggcgcc ccucuccagc      960

uccaugguga accgcuacga ggagcaccag aaggaccugg cccugcucaa gaacuucauc     1020

caccagaacc ugagcgacag cuacaaggag guguucaacg acaagcugaa ggacggcuac     1080

gccggcuaca ucgaggggaa gaccacgcag gagaacuucu accgguucau caagaaggcc     1140

aucgagaaga ucgacggcuc caacuacuuc aucgagaaga ucgagcgcga ggacuuccuc     1200

cggaagcagc gcaccuucga caacgggagc aucccccacc agauccaccu ccaggagaug     1260

caggccauca uccggcgcca ggcgaaguuc uaccccuucc uggaggagaa ccaggacaag     1320

aucgagaaga uccucaccuu ccgcaucccg uacuacgucg gcccccuggc ccgggggaag     1380

uccgaguucg ccuggcugaa ccgcaagagc gacgagaaga uccggcccug gaacuucgac     1440

gagauggugg acaaggagac cuccgccaag aacuucauca cgcgcaugac caacuacgac     1500

caguaccucc ccgaccagaa ggugcugccg aagcacagcc ugcuguacga gaaguucgcc     1560

gucuacaacg agcucaccaa ggugcgguac gugaccgagc agggcaaguc cuucuucuuc     1620

gacgcgaaca ugaagcagga gaucuucgac ggcgucuuca agguguaccg caaggugacg     1680

aaggagaagc ugauggacuu ccuggggaag gaguucgacg aguuccggau cgucgaccuc     1740

cugggccugg acaaggagaa caagagcuuc aacgccuccc ucgggaccua ccacgaccug     1800

aagaagaucg ugagcaagga cuuccuggac aacuccgaga acgaggacau ccucgagaac     1860

gucgugcuga cccugacccu cuucgaggac cgcgagauga uccggaagcg ccuggagaag     1920

uacaaggaca uccugacgga ggagcagcgg aagaagcucg agcgccggca cuacaccggc     1980

ugggggcgcc ugagcgccaa gcugaucaac ggcauccggg acaaggugac ccgcaagacc     2040

auccucgacu accugaucga cgacggcacg uccaacugca acuucaugca gcugaucaac     2100

gacgacaccc ucagcuucgu cgacgagauc cggcuggccc agggguccgg cgaggccgag     2160

gacuaccgcg cggaggugca gaaccuggcc gggagccccg ccaucaagaa gggcauccuc     2220

cagucccuga agaucgugga cgagcugauc gaggucaugg gguacgaccc cgagcacauc     2280

gugguggaga uggcccggga gaaccaguuc accaaccagg gccgccgcaa cagccagcag     2340

cgguacaaga agaucgagaa cgcgaucaag aaccucgacu ccaaccugaa cagcaagauc     2400

cugaaggagu accccaccaa caaccaggcc cuccagaacg accgccuguu ccuguacuac     2460

cuccagaacg gcaaggacau guacacggac gaggagcugg acaucgacca gcugagccag     2520

uacgacaucg accacaucau cccgcaggcc uucaucaagg acgacucccu cgacaacaag     2580

guccugacca agagcgccaa gaaccggggg aaguccgacg acgugcccag ccuggagauc     2640

guguacaaga agaaguccuu cuggaagcag cuccuggaca gccagcugau cucccagcgc     2700

aaguucgaca accucaccaa ggccgagcgg ggcgggcuga ccaacgagga caaggcgcgc     2760

uucauccagc ggcagcuggu cgagacccgc cagaucacga agcacguggc ccggaugcuc     2820

gacacccgcu ucaacaccaa gcuggacgag gccggcaacc ggauccgcga ccccaaggug     2880

aacaucauca cccugaaguc caaccucguc ucccaguucc ggaaggacua ccagcuguac     2940

aaggugcgcg agaucaacaa cuaccaccac gcccacgacg ccuaccugaa cgcggugguc     3000

gccacggccc uccugaagaa guacccccag cuggccccgg aguucgugua cggcgaguac     3060

cccaccuaca acagcuacaa cucccgcaag agcgccaccg agaaggugcu guucuacucc     3120

aacaucauga acuucuucaa gcggaucguc gugcacagca agaccgggga ggugcggauc     3180

cgccccguca ucgaggugaa caaggagacg ggcgagaucg ugugggacaa gaaguccgac     3240

uuccggaccg uccgcaaggu gcugagcuac ccccagguga acgucgugaa gaagaccgag     3300

gugcagaacc acgggcugga ccggggcaag ccgaaggggu ucuacaacgc gaaccucagc     3360

ccccgcccca aggagggcag cgucgagaac cuggugcccg ccaagcagag cuucgacacc     3420

aagcgcuacg gcggguacgc cggcaucucc aacagcuacg ccgugcuggu caacgggauc     3480

aucgagaagg gcaagaagaa guccaaggcg gaggugacgg aguuccaggg gaucagcauc     3540

cucgcccgga aggacuucga caagaacccg aagcaguacc ugcugaaccu cggcuacaag     3600

gacaucaagu ccaucaucaa gcugcccaag uacagccugu ucgagcucga gaacggcucc     3660

cgccggaugc uggccagcau ccuguccacc aacaacaagc gcggggagau ccacaagggc     3720

aacgagaugu accuccccga caaguucgug acccugcugu accacgccau gcgggucaac     3780

cgcacccucg agccggggca caagaaguac guggagacgc accggcacgu guucgacgag     3840

cugcugaucu acauccucga guacaacgag aagaucgucg gcgccaaggc gaacgggaag     3900

cgcaucgugg aggccuacag cgcccggaag gacaccgaca accuggagga gcugugcaac     3960

uccuucauca accuccugaa ccugaccgcc cucggcagcg ccguggacuu cgaguuccug     4020

ggcaccaaga ucccccgcua ccgggacuac acgcccucca gccugcucaa ggcgacccug     4080

auccaccagu ccgucaccgg gcuguacgag acccgcaucg accucagcaa gcugggcggg     4140

gacuga                                                                4146


<210>  2058
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2058
augaagaaga ucgugaacua caaccugggc cucgacaucg ggaccagcuc caucggcuac       60

gccgccaucg acgaccaggg gcgccccguc cggcaccaga acaagaccgu gaugggcgug      120

cgccuguuca aggaggggaa gacggcggag agcacccgga ucuuccgcgg cacccggcgc      180

cggcuguccc accgccacug gcggcucggc uaccuggacc gcaucuucga caacgagauc      240

gccaaggucg acgagaacuu ccugccgcgg cucaaggcca gcaagcgcgc ccgguccgac      300

gagaagcgca gcuuccgggg gucccugcug uuccccgaga ccggcgacga cgccuacuac      360

cagaaguacc ccacgaucua ccaccuccgc uacgcgcuga ucacccagaa gaagcaguuc      420

gacauccggc ugaucuaccu cgcccugcac cacaugauca aguaccgcgg gaacuuccug      480

gacaccaccc ccgugagcgc cuucaacauc cacgacaucg accucacgga ccaguucgcc      540

ucccugaacg acugcuacca gcugcucgac auggacuucg aguuccaguu cgaccugaac      600

aacaucgagc agguccagaa gcugcuccug gacgagaccc ugagcaagac caagcggcag      660

gcggccgugu ccaaggugcu cgugaugagc accggcaaca aggccuucga cgaccagcag      720

aacgggaucg ccuccgaguu caucaaggcc cugcugggcg gcccguucga ccucagcaag      780

auccugaacc uguccgucgc ggacgucaag agcuggaagc uccacaugac ggacaaggac      840

guggacgaca cccugaacuc ccugguggag gagcucgacg agaaccacaa gcagaucgug      900

aaccugcugc agaugaucua cagccaggug augaugcucg ggcugcugaa cggccagcag      960

uccaucagcg ccagcaugau cgagcgcuac gacgaccacg ccaagcacug ggccgcccuc     1020

aagaagcugc ugcccgcgcu cggggagcag gagcagucca ccaucaucca cgccuacacc     1080

gccuacacgg gccacagccg gaccaagcag cugucccgcg ccgaguucua caccaagguc     1140

aacagcaucc ugaagaagca cgagaacauc gccgaggcga ccggccucau cgagcugauc     1200

gaccgcaacg aguucaugcc caagcagcgg gacgccguga acggggugau cccccaccag     1260

cugcaccagc gcgagcucca gcugaucauc cagaaccagc agaaguacua cccguuccug     1320

gccgccaagg ccuaccccga cgcggacgag acgaugaucg agcagcugcu cuccuuccgg     1380

guccccuacu acgccggccc ccugguggac cgcgacuuca ucaacgagag cuccgaccag     1440

gagcaggcca ugcagaacac caaguucagc uggcugaagc ggcgcgaggc cggggagauc     1500

accccgugga acuucgagca gaagguggac guccaggaga ccgcgaaccg guucaucaag     1560

cgccucacga ccaaggacac cuaccugaug accgaggacg ugcugcccaa ggagucccuc     1620

aucuaccagc gguucgaggu gcugaacgag cugaacgcca uccgcaucaa cgaccggcgc     1680

cucacggccg agaacaagca gcuggccuuc cagcagcugu ucauggccgg caacaccaag     1740

aacgucagca ucaagaaguu ccggaacuuc guguucgacu cccucaaguu ccccgagaag     1800

gucaagauca ccggguucgc gggccagcac aacuuccugg ccaagcugag caccuaccac     1860

gacuucaaga agaucuucgg cgagcgcgug gaccacacgg accuccagcc cgacuucgag     1920

cggaucgugg aguggugcac caucuucgag gaccgccaca ucuaccggga gaagcugaag     1980

gagauccagu ggcuguccca gagcgagauc gaccagcucg uccugauccg caaccacggg     2040

uggggccggc uguccaagcg ccuccugacc gggauccgcg acagcgacgg ccggaccgug     2100

auggaccagc ugugguucgg gacggacaac uucaugcgca ucaucuccca gagcgacuuc     2160

gccgccgcca ucgcgaacga gaaccggacc cagcucaacc agcacggccu ggaggacgug     2220

cuggacuccg ccuucaugag cccgcagaac aagaaggcca uccgccaggu ccucaaggug     2280

guggacgaca ucgagcagac ccugggcugc ccgcccaaga aggucaugau cgaguucacc     2340

cggagccccg acaagaagaa gcagcgcacc gugacgcggc uggagaaggu gcagcaggcc     2400

uacaaggccg ucgcggacca gcucgccggg caguccaucg ugagcgagcu ggaccaguac     2460

gccaacaccc acuccaagcu gagcgacacc uacuaccucu acuucaugca gcugggccgc     2520

gaccuguacu ccgggaagcc caucgacauc gacagcccca ugcucgacga cgaccacauc     2580

cugccccggg ccuucaugaa ggacaacucc cuggacaacc gcgcgcucac cgagaagccc     2640

aucaacaaca cgaaggccgc caaguucccg agcaaggugu ucggccagca gaaggagguc     2700

accgacuucu ggaagcugcu gcggcgcugc gggcucaugu ccaacaucaa ggagaagcac     2760

cuguaccugg accccgacaa cguggacaag uggacccggc gcggcuucau ccaccggcag     2820

cucguggaga ccagccaggu caucaagauc guggccaaca uccugaacga ccgcuaccag     2880

cagucccaga cggaggugcu ggaggucccc gcccggcucg gcaccgagcu gcgcgagugc     2940

uucggguggu acaaggugcg ggaggugaac gacuaccacc acgucuucga cgcguaccug     3000

gccgccuuca ucggcaacua ccucuaccgc cgguacccca agcugcagcc guacuucgug     3060

uacgggaacu ucgccgagcu gaacaaggcc cagcucaaga acaucaagag cuucaacuuc     3120

cuguacaaca ucaccgacaa caagcgcgug gucaaggaca ucgagaccgg ccagacgcug     3180

ugggacgacc agauccgcca gcagauccag cacaucuacg aguacaagua caugcucacc     3240

ucccacgagg cguucaccca gcacaaggag cuguacggcc agaccaucua cccccggcgc     3300

gaguucaagc ccgggacgcu gaaggccauc aagcaggacc agccgcagga gcucuacggc     3360

ggguacaaga accugaccgc cgccuacaug ugcaucguga agauccaggc ccggaagcgc     3420

cgggcggccg aguaccgcgu gguccgggug gccaccgaga gccuguccgc ccuccagcag     3480

gcgaagcgcc ugggcgugca ggccuacgag cggugccugc gccaggagcu cguccggaac     3540

aacgagaucc ugcgcaccaa cgccgacccc aacagcgcgc cccgggaguu cgaggugcug     3600

cucgaccgcg ugcugugcca ccagaugauc aucuccgacg ggcagaagua ccugcucggc     3660

agccacgccu acaagaucaa cugcaugcag cugguccuga gcaacaacuc caugaagauc     3720

cucagcacgu ccaucgugaa gcugaagcag augaccaacg agccccuggu gcaggucuac     3780

cgggagaucc ucgacguggu gaacaaguac uucacccugu acgacgucaa cagcuuccgc     3840

gagaaccuga accaguucuu cgacaaguuc cggacccucc cgaucacgaa cgaguacgag     3900

uccagcaagu ccggcaagaa gcugauccac cgcgggcagc uggagacccu cacccugauc     3960

cugcggggcc uccacgccaa cagcgacaag gugcaguacg gggaccaguc cacccugcug     4020

ggcggguucc agacgaagga cggcaucaag cucagcaagg acuccgugau ccaguacacc     4080

agccccgccg gccugaacgu ccgcaccgug aagcugcgcg accucgagca gaaggaggcc     4140

cagaaguga                                                             4149


<210>  2059
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2059
augguggacg agaagaucuu cuggggccug gacaucggga ccgacagcgu cggcugggcc       60

gugaccaacu ccgaguacaa gcucaagaag uacaagaaca accugaugug gggcgugcac      120

cuguucgacg aggccaagca gagcgcggag cgccgguccu uccgcacggc ccggcgccgg      180

cucgaccgcc ggaagcagcg caucauccug cugcaggagu ucuucguccg ggccgugugc      240

gagaaggacg agaacuucuu ccgccggcuc aaggagagcg cccugcugcc cgaggacgcc      300

gagcaccgca ccaacaacau cuucuucgac gacccggacu acaccgacaa ggacuacuuc      360

gaggaguacc ccaccaucca ccaccucauc ugcgagcuga uggaguccaa ggagccccac      420

gacgugcggc uggucuaccu cgcgugcgug uaccugcugg cccaccgcgg gcacuuccuc      480

cugcccguga gcgaggacga caucuccaag gucacggagu ucgagccgcu guacgagagc      540

uucuacaagg cccucgagga gaagcuggac gacgagccgc ccuucgaccg guccgccgac      600

gacuucgcgg agauccucaa gagccacaag accguguccg ccaagaacaa ggacuucgac      660

aagcuccugu ucgggggcaa ggugaagacc uacgacaacg agaacaucag cuacuccgcc      720

cugaucaagc uccugagcgg cgggaccgag aagcugucca aguucuucgc caacgaggag      780

uacacggacc ucgagaagga cagcgucugc gugcgcaacg ccgacuucgg cgacacccug      840

gagaugcugg aggggcagau cgacgagcuc gacuucgcgc ugcugaaguc cgucaagagc      900

cucuacgacu ggucccugcu gguggacauc cucgagggca agagccugau cagcgaggcc      960

aagaaggaca aguacgacga gcacggguac gaccuggacg cccucaagua ccuguuccgg     1020

gaguaccuga ccaaggacga cuacaacgag auguucaagg agguguccgg caagcagaac     1080

uacgccagcu acguguacaa cgccuccagc gacaagaccc gcgacuccaa guacaagaag     1140

ugcagccaga aggacuucug caaguucacg aagaaguucc ucuccaagau caagcccaac     1200

gagaaggaca agcccugccu ggacaagcug cucgagaagu gcgagcagaa cagccugugc     1260

cccaagcagg ucaccaccga caaccgggug auccccuacc agcuguacua cguggagcuc     1320

aagaagaucc uggagaacgc gugcggcuac cugcccuucc ugaacgagcg cgacgaguac     1380

gggaccgucg ccgacaagau ccucuccauc augaaguucc gggugccgua cuacgugggc     1440

ccccuggucg acagcaagaa gucccccaac gccuggcugg ugcgcaagcu cgacgggaag     1500

aucacgcccu ggaacuucac cgacaugguc aacgaggacg agagcgagaa cgccuucauc     1560

cgccggauga ccugcaagug caccuacgug gccggccagg acgugcugcc gaaguacucc     1620

cugcucuaca gcaaguucuc cguccugaac gagaucaaca acaucaagcu gaacggcgag     1680

cccaucagcg ugcagacgaa gcaggagauc uacaccgagc uguucgagcg caacaagucc     1740

cgggugagca agaagaagau ccgcgacugc cugaucuccc acggguacgc ggccgacagc     1800

gacgagguca ccggcaucga cgacaucgcc aagagcgccc ugcgguccua ccacgacuuc     1860

aagaagaugc ucagcaacgg gauccugacc gagcagcagg ucgaggagau caucgagcac     1920

aucacgguga ccaccgacaa cauccgccug aagaaguggc ucaagaccca guuccccaug     1980

cuggccgacg aggacgugaa guacaucacg aagcugaagu acaaggacua cggccggcuc     2040

ucccgcugcu uccuggagga cgugcugccc guggacacca agaccgggga ggcggagagc     2100

gacaagaaca ucaucaccau gcucugggag acgaacgaga acaucaugca gcugcugucc     2160

agcaaguacc gguacuccga gaacaucgag cacaugaacc gccaguacua cgcccucccg     2220

gagaaccaca agagcauguc cgagcggcug aaggacaugu acguccccac cgccgugcgc     2280

cgggccguga cccgcaccuu cgacaucguc aaggagcuga agaagaucca gggccgggac     2340

cccgacaaga ucuucaucga gauggcgcgc ggcacggggg agacccccaa gggcaagcgg     2400

accaacagcc gcaaggacca gauccucgag cacuggcacg ggcuggacaa caaggacauc     2460

aacgaccuga agaaguccgg caucugggag caccucgaca ccaucgacga cgccaagcug     2520

cggagcgaca aguacuuccu guacuucaug cagcucgggc gcugcaugua caccgagaag     2580

ccgauccccu ucgaggaggu ggagaacgag cacaagugga acaucgacca caucuggccc     2640

caggccaaga ucaaggacga cucccuggac aacaaggugc uggucagcuc caacgagaac     2700

ggcaagaagu ccgacagcua ccccaucagc gacgacaucc gccacagcau ggccggccuc     2760

uggcacuccc uguacaagaa ggggcugauc agcgagaaga aguaccagcg gcucacgcgc     2820

uccaccccgu ucaccgacga cgagcugagc ggcuucaucg cccggcagcu gguggagacc     2880

cgccagucca cgaaggcggu ggccacccuc cugaaggagc aguuccccaa caccgagauc     2940

gucuacguga aggccgggcu ggugagcgac uuccggcagg agaugggcau gcucaagugc     3000

cgcgagguca acgaccugca ccacgccaag gacgccuacc ugaacaucgu gcucgggaac     3060

guguacaaca cccgguucac gaaggacccc cugaacuucg ucaagaacaa cgagaaguac     3120

uccaucaaga ucuuccagaa gaacagcgac ggcaagaaga ccggcgugau cacccgcaag     3180

guggagcgcg ggggcgaggu cgcgugggac ccggagaccu ccuucgccau cgugcggaag     3240

augaugagca agaacuccau ccgguacgug cgcuacgccu acaugcgcaa gagcgggcag     3300

aacggcggcu ucuucgacca gaacccccug cggaagggga agggcauggu cuccaucaag     3360

cacaaccuca gcaucgagaa guacgggggc uacgacaacg ccacgaucuc cuucuucagc     3420

cuggugucca ucaaggacuu cggggugauc cugcucgccg ucgagcugcu gagcgcgaac     3480

aaguuccuca agaacaucuc cgaggccaag gaguacgccg ccgacaagcu gagcaaggag     3540

cugaacguga agaucgugcc caacgacguc gcguuccccc ucaacaaccg guacaacgug     3600

gugaagauca acgccguccu ggagcuggac ggcuaccgcg ugaugaucgc cgccaagcag     3660

aacagcucca ucaaguucgu gagcaacgag ucccucaucc ugagcuccca gcacauggcc     3720

uacgucaaga agaucaccaa guucaucgag aagcggaagc aggacaaggu gugcaugauc     3780

aacgcguuca gcggcaucuc caucgaggac aacaucgagc uguacgacgu gcucgucagc     3840

aagugcaaca ucaagccguu caacaucaug uacgagaacc uggggaucaa gcuguccgac     3900

aaccgcgaca acuucaucaa gaaguccgug gagaaccagg ugcucauccu gagcgagaug     3960

guccggcugu ucaagaccgg ccgcaaggcc ggggugaacc ucgaggaggu cggcgggcgg     4020

aagaacagcg gcgugaagac ccugaacacg ugccuguaca aggugaagga cuacuccaac     4080

aucuuccucg ucgaccagag ccccaccggc cugaucgagc acaagucccc caaccugcuc     4140

gaccuguga                                                             4149


<210>  2060
<211>  4155
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2060
augaacaagc ccuacagcau cggccuggac aucgggacca acuccguggg cuggagcauc       60

aucaccgacg acuacaaggu cccggccaag aagaugcgcg ugcucgggaa cacggacaag      120

gaguacauca agaagaaccu gaucggcgcc cuccucuucg acgggggcaa caccgcggcc      180

gaccggcgcc ugaagcggac cgcccgccgg cgcuacaccc ggcgccggaa ccgcauccug      240

uaccuccagg agaucuucgc cgaggagaug uccaaggugg acgacagcuu cuuccaccgg      300

cuggaggacu ccuuccuggu cgaggaggac aagcgcggca gcaaguaccc caucuucgcc      360

acgcuccagg aggagaagga cuaccacgag aaguucucca ccaucuacca ccuccggaag      420

gagcuggcgg acaagaagga gaaggccgac cugcgccuga ucuacaucgc ccuggcccac      480

aucaucaagu uccgggggca cuuccucauc gaggacgaca gcuucgacgu gcgcaacacc      540

gacaucucca agcaguacca ggacuuccug gagaucuuca acaccacguu cgagaacaac      600

gaccugcuca gccagaacgu ggacgucgag gcgauccuga ccgacaagau cuccaagagc      660

gccaagaagg accggauccu ggcccaguac cccaaccaga aguccaccgg caucuucgcc      720

gaguuccuca agcugaucgu ggggaaccag gccgacuuca agaaguacuu caaccuggag      780

gacaagacgc cccuccaguu cgcgaaggac agcuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacgaguu cgccgaccug uucuccgccg ccaagaagcu guacgacagc      900

gugcuccugu ccggcauccu gaccgucauc gaccucagca ccaaggcccc gcuguccgcg      960

agcaugaucc agcgcuacga cgagcaccgg gaggaccuga agcagcucaa gcaguucgug     1020

aaggccagcc ugcccgagaa guaccaggag aucuucgccg acuccagcaa ggacggcuac     1080

gccggguaca ucgagggcaa gaccaaccag gaggccuucu acaaguaccu guccaagcuc     1140

cugaccaagc aggaggacag cgagaacuuc cuggagaaga ucaagaacga ggacuuccuc     1200

cgcaagcagc ggacguucga caacgggucc aucccccacc aggugcaccu gaccgagcug     1260

aaggcgauca uccgccgcca gagcgaguac uaccccuucc ucaaggagaa ccaggaccgg     1320

aucgagaaga uccugaccuu ccgcaucccg uacuacaucg gcccccuggc ccgggagaag     1380

uccgacuucg ccuggaugac ccgcaagacg gacgacagca uccggcccug gaacuucgag     1440

gaccucgucg acaaggagaa guccgccgag gccuucaucc accgcaugac caacaacgac     1500

uucuaccugc ccgaggagaa ggugcugccg aagcacagcc ugaucuacga gaaguucacc     1560

guguacaacg agcucaccaa gguccgguac aagaacgagc agggcgagac guacuucuuc     1620

gacuccaaca ucaagcagga gaucuucgac gggguguuca aggagcaccg caaggugagc     1680

aagaagaagc ugcuggacuu ccucgcgaag gaguacgagg aguuccggau cgucgacgug     1740

aucggccugg acaaggagaa caaggccuuc aacgccuccc uggggaccua ccacgaccuc     1800

gagaagaucc uggacaagga cuuccuggac aaccccgaca acgagagcau ccucgaggac     1860

aucgugcaga cccugacccu guucgaggac cgcgagauga ucaagaagcg gcucgagaac     1920

uacaaggacc uguucacgga gucccagcug aagaagcucu accgccggca cuacaccggc     1980

ugggggcgcc ugagcgccaa gcugaucaac ggcauccggg acaaggaguc ccagaagacc     2040

auccucgacu accugaucga cgacggccgc agcaaccgga acuucaugca gcugaucaac     2100

gacgacgggc gcuccaaccg gaacuucaug cagcucauca acgacgacgg ccugagcuuc     2160

aagagcauca ucuccaaggc gcaggccggg agccacuccg acaaccugaa ggaggucgug     2220

ggcgagcucg ccgggagccc cgccaucaag aagggcaucc ugcagucccu gaagaucgug     2280

gacgagcucg ucaaggugau gggcuacgag cccgagcaga ucgucgugga gauggcccgc     2340

gagaaccaga ccacgaacca ggggcgccgg aacagccgcc agcgguacaa gcugcuggac     2400

gacggcguga agaaccucgc guccgaccug aacgggaaca uccugaagga guacccgacc     2460

gacaaccagg cccuccagaa cgagcgccug uuccuguacu accuccagaa cggccgggac     2520

auguacaccg gggaggcccu ggacaucgac aaccugagcc aguacgacau cgaccacauc     2580

aucccccagg ccuucaucaa ggacgacucc aucgacaacc gcguccucgu gagcuccgcc     2640

aagaaccggg gcaagagcga cgacgugccc ucccuggaga ucgucaagga cugcaaggug     2700

uucuggaaga agcugcucga cgcgaagcug augagccagc gcaaguacga caaccugacc     2760

aaggccgagc ggggcgggcu cacguccgac gacaaggccc gcuucaucca gcggcagcug     2820

guggagaccc gccagaucac caagcacguc gcccggaucc uggacgagcg cuucaacaac     2880

gagcucgaca gcaagggccg gcgcauccgg aaggugaaga ucgugacccu gaaguccaac     2940

cuggucagca acuuccgcaa ggaguucggg uucuacaaga uccgggaggu gaacaacuac     3000

caccacgccc acgacgcgua ccucaacgcc guggucgcca aggccauccu gaccaaguac     3060

ccccagcugg agccggaguu cguguacggc gacuacccca aguacaacag cuacaagacg     3120

cgcaaguccg cgaccgagaa gcuguucuuc uacagcaaca ucaugaacuu cuucaagacc     3180

aaggugaccc uggccgacgg cacggucgug gugaaggacg acaucgaggu caacaacgac     3240

accggggaga ucguguggga caagaagaag cacuucgcca ccgugcggaa gguccugucc     3300

uacccccaga acaacaucgu gaagaagacc gagauccaga cgggcggguu cagcaaggag     3360

uccauccucg cccacggcaa cagcgacaag cugauccccc gcaagaccaa ggacaucuac     3420

cuggacccga agaaguacgg gggcuucgac ucccccaucg uggccuacag cguccucgug     3480

guggcggaca ucaagaaggg caaggcccag aagcugaaga ccgucaccga gcugcucggg     3540

aucacgauca uggagcgcuc ccgguucgag aagaacccca gcgccuuccu ggaguccaag     3600

ggcuaccuga acauccgcgc cgacaagcuc aucauccugc ccaaguacag ccuguucgag     3660

cucgagaacg ggcggcgccg gcugcuggcc uccgcgggcg agcuccagaa ggggaacgag     3720

cuggcccugc cgacccaguu caugaaguuc cucuaccugg ccagccgcua caacgagucc     3780

aagggcaagc ccgaggagau cgagaagaag caggaguucg ugaaccagca cgugagcuac     3840

uucgacgaca uccugcagcu caucaacgac uucuccaagc gggucauccu ggccgacgcc     3900

aaccuggaga agaucaacaa gcucuaccag gacaacaagg agaacaucag cguggacgag     3960

cuggcgaaca acaucaucaa ccuguucacc uucaccuccc ucggcgcccc cgccgccuuc     4020

aaguucuucg acaagaucgu ggaccgcaag cgguacacga gcaccaagga gguccugaac     4080

agcacccuga uccaccaguc caucaccggg cucuacgaga cgcgcaucga ccugggcaag     4140

cugggggagg acuga                                                      4155


<210>  2061
<211>  4155
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2061
augaagcugc gcggcaucga ggacgacuac agcaucgggc ucgacauggg caccuccagc       60

gugggguggg ccgucaccga cgagcggggc acgcuggccc acuucaagcg caagcccacc      120

uggggguccc ggcuguuccg cgaggcgcag accgccgccg uggcccggau gccgcgcggc      180

cagcggcgcc gguacgugcg ccggcgcugg cggcucgacc ugcugcagaa gcuguucgag      240

cagcagaugg agcaggccga ccccgacuuc uucauccgcc uccggcagag ccgccugcuc      300

cgggacgacc gcgcggagga gcacgccgac uaccgguggc cccuguucaa cgacugcaag      360

uucaccgagc gcgacuacua ccagcgguuc cccacgaucu accacguccg cuccuggcug      420

auggagaccg acgagcaggc cgacauccgc cucaucuacc uggcccugca caacaucgug      480

aagcaccggg gcaacuuccu ccgcgagggg cagagccugu ccgccaagag cgcgcggccg      540

gacgaggccc ugaaccaccu ccgcgagacc cugcgggugu gguccagcga gcgcggcuuc      600

gagugcucca ucgccgacaa cgggagcauc cuggccaugc ucacccaccc cgaccugucc      660

cccagcgacc ggcgcaagaa gaucgcgccc cuguucgacg ucaaguccga cgacgccgcc      720

gccgacaaga agcucggcau cgcccuggcg ggggccguga ucggccugaa gacggaguuc      780

aagaacaucu ucggcgacuu cccgugcgag gacagcucca ucuaccucag caacgacgag      840

gccguggacg ccguccgguc cgccugcccc gacgacugcg cggagcuguu cgaccgccug      900

ugcgaggugu acagcgccua cgugcuccag gggcugcuga gcuacgcccc cggccagacc      960

aucuccgcca acauggucga gaaguaccgg cgcuacgggg aggaccucgc ccugcugaag     1020

aagcucguga agaucuacgc gcccgaccag uaccggaugu ucuucagcgg cgccaccuac     1080

ccgggcaccg ggaucuacga cgccgcccag gcgcgcggcu acacgaagua caaccugggg     1140

cccaagaagu ccgaguacaa gcccagcgag uccaugcagu acgacgacuu ccggaaggcc     1200

guggagaagc uguucgccaa gaccgacgcc cgcgccgacg agcgguaccg caugaugaug     1260

gaccgguucg acaagcagca guuccuccgc cgccugaaga ccagcgacaa cggcuccauc     1320

uaccaccagc ugcaccucga ggagcugaag gcgaucgucg agaaccaggg gcgguucuac     1380

cccuuccuga agcgcgacgc cgacaagcuc gugagccugg uguccuuccg gaucccguac     1440

uacgucggcc cccugagcac ccgcaacgcc cggacggacc agcacggcga gaaccgcuuc     1500

gccugguccg agcggaagcc cgggaugcag gacgagccca ucuucccgug gaacugggag     1560

agcaucaucg accgcuccaa gagcgccgag aaguucaucc uccggaugac cggcaugugc     1620

accuaccugc agcaggagcc cgugcugccc aaguccagcc ugcucuacga ggaguucugc     1680

gugcugaacg agcugaacgg ggcgcacugg uccaucgacg gcgacgacga gcaccgcuuc     1740

gacgccgccg accgggaggg gaucaucgag gagcuguucc gccggaagcg caccgucagc     1800

uacggcgacg uggccggcug gauggagcgg gagcgcaacc agaucggggc ccacgugugc     1860

ggcgggcagg gcgagaaggg guucgagucc aagcucggca gcuacaucuu cuucugcaag     1920

gacgucuuca agguggagcg gcuggagcag agcgacuacc ccaugaucga gcgcaucauc     1980

cucuggaaca cgcuguucga ggaccggaag auccuguccc agcgccucaa ggaggaguac     2040

ggcagccggc uguccgcgga gcagaucaag accaucugca agaagcgcuu caccgggugg     2100

ggccgccuga gcgagaaguu ccucaccggg aucacggucc agguggacga ggacuccgug     2160

agcaucaugg acguccugcg ggagggcugc ccgguguccg gcaagcgcgg gcgggccaug     2220

gugaugaugg agauccugcg cgacgaggag cucggcuucc agaagaaggu cgacgacuuc     2280

aaccgggccu ucuucgccga gaacgcgcag gcccuggggg ugaacgagcu gcccggcagc     2340

cccgccgugc gccggucccu caaccagagc auccgcaucg ucgacgagau cgccuccauc     2400

gccgggaagg cgcccgccaa caucuucauc gaggugaccc gggacgagga cccgaagaag     2460

aagggccgcc ggaccaagcg ccgguacaac gaccugaagg acgcccugga ggccuucaag     2520

aaggaggacc ccgagcucug gcgcgagcug ugcgagaccg cccccaacga cauggacgag     2580

cggcugagcc ucuacuucau gcagcgcggc aagugccugu acuccgggcg ggcgaucgac     2640

auccaccagc ugagcaacgc cggcaucuac gagguggacc acaucauccc gcgcacguac     2700

gucaaggacg acucccucga gaacaaggcc cugguguacc gggaggagaa ccagcgcaag     2760

accgacaugc ugcucaucga ccccgagauc cgccggcgca ugagcgggua cuggcggaug     2820

cugcacgagg ccaagcugau cggcgacaag aaguuccgca accuccugcg gucccgcauc     2880

gacgacaagg cccugaaggg guucaucgcg cggcagcucg uggagaccgg ccagaugguc     2940

aagcuggugc gcagccugcu cgaggcccgg uaccccgaga ccaacaucau cagcgugaag     3000

gccuccauca gccacgaccu gcgcacggcc gcggagcugg ucaagugccg ggaggccaac     3060

gacuuccacc acgcccacga cgccuuccuc gccugccgcg ugggccuguu cauccagaag     3120

cggcaccccu gcguguacga gaacccgauc gggcuguccc aggucgugcg caacuacgug     3180

cggcagcagg cggacaucuu caagcgcugc cggaccaucc ccggcagcuc cggguucauc     3240

gucaacagcu ucaugaccuc cggcuucgac aaggagaccg gggagaucuu caaggacgac     3300

ugggacgccg aggccgaggu ggagggcauc cgccggagcc ucaacuuccg ccagugcuuc     3360

aucucccgga ugcccuucga ggaccacggc guguucuggg acgccacgau cuacagcccc     3420

cgcgccaaga agaccgcggc ccugccgcug aagcaggggc ucaaccccuc ccgcuacggc     3480

agcuucuccc gggagcaguu cgccuacuuc uucaucuaca aggcccgcaa cccccggaag     3540

gagcagaccc uguucgaguu cgcccagguc cccgugcgcc ugagcgcgca gauccggcag     3600

gacgagaacg cccucgagcg cuacgcccgg gagcuggcca aggaccaggg gcuggaguuc     3660

auccgcaucg agcgguccaa gauccucaag aaccagcuga ucgagaucga cggcgaccgc     3720

cugugcauca ccgggaagga ggaggugcgg aacgccugcg agcucgcguu cgcccaggac     3780

gagaugcgcg ucauccggau gcuggugagc gagaagccgg ugucccgcga gugcgucauc     3840

agccuguuca accggauccu ccugcacggc gaccaggccu cccgccggcu gagcaagcag     3900

cucaagcugg cccugcucag cgaggcguuc uccgaggcca gcgacaacgu gcagcgcaac     3960

gugguccugg gccugaucgc caucuucaac ggguccacca acauggugaa ccucagcgac     4020

aucggcgggu ccaaguucgc cggcaacgug cggaucaagu acaagaagga gcuggccagc     4080

cccaagguca acgugcaccu gaucgaccag uccgugacgg gcauguucga gcgccgcacc     4140

aagaucgggc ucuga                                                      4155


<210>  2062
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2062
augaccaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucacggacg acuacaaggu gccgagcaag aagaugaagg ugcucgggaa caccuccaag      120

aaguacauca agaagaaccu gcugggcgcc cuccuguucg acagcgggau caccgcggag      180

ggccgccggc ugaagcgcac cgcccggcgc cgguacacgc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucuc caccgagaug gccacccugg acgacgccuu cuuccagcgc      300

cucgacgaca gcuuccuggu ccccgacgac aagcgggacu ccaaguaccc caucuucggc      360

aaccuggugg aggagaaggu guaccacgac gaguucccca ccaucuacca ccuccgcaag      420

caccuggccg acagcuccaa gaaggcggac cugcggcucg ucuaccuggc ccuggcccac      480

augaucaagu accgcgggca cuuccucauc gagggcgacu ucaacagcaa gaacaacgac      540

cugcagaaga acuuccagga cuuccuggac acguacaacg ccaucuucga guccgaccuc      600

agccuggaga acuccaagca gcuggaggag aucgugaagg acaagaucag caaguccgcg      660

aagaaggacc ggauccucaa gcuguucccg ggggagaaga acagcggcau cuucuccgag      720

uuccugaagc ucaucguggg gaaccaggcc gacuuccgca aguacuucaa ccuggacgag      780

aagaccagcc ugcaguucuc caaggagagc uacgacgagg accucgagac ccugcugggc      840

cacaucggcg acgacuacag cgacgucuuc cucaaggcca agaagcugua cgacgccauc      900

cugcucuccg ggauccugac cgugacggac aacggcaccg aggcgccccu gagcuccgcc      960

augaucaugc gguacaagga gcacgaggag gaccucgccc ugcugaaggc cuacauccgc     1020

aacaucagcc ucgagaccua caacgaggug uucaaggaca acaccaagaa cggguacgcc     1080

ggcuacaucg acggcaagac gucccaggag gacuucuacg ucuaccugaa gcggcugcuc     1140

gccgggcugg agggcgcgga cuacuuccug gagaagaucg accgcgagga cuuccuccgg     1200

aagcagcgca ccuucgacaa cgggagcauc cccuaccaga ucuaccugca ggagaugcgc     1260

gccauccugg acaagcaggc caaguucuac cccuuccugg ccaagaacaa ggagcggauc     1320

gagaagaucc ucaccuuccg caucccguac uacgugggcc cccuggcccg ggggaacucc     1380

gacuucgcgu ggagcauccg caagcggaac gagaagauca cccccuggaa cuucgaggac     1440

gugaucgaca aggaguccag cgccgaggcc uucaucaacc gcaugacguc cuucgaccug     1500

uaccuccccg aggagaaggu ccugccgaag cacagccugc ucuacgagaa cuucaccgug     1560

uacaacgagc ugaccaaggu gcgguucauc gccgagggca ugcgcgacua ccaguuccug     1620

gacuccaagc agaagaagga caucguccgg cucuacuuca agggcaagcg caaggugacc     1680

gacaaggaca ucaucgagua ccugcacgcc aucgacgggu acgacggcau cgagcugaag     1740

gggaucgaga agcaguucaa cagcucccuc agcacguacc acgaccugcu gaacaucauc     1800

aacgacaagg aguuccucga cgacuccagc aacgagacca ucaucgagga gaucauccac     1860

acccugacca uguucgagga ccgggagaug aucaagcagc gccuguccaa guucgacaac     1920

aucuucgaca agagcgugcu caagaagcug agccggcgcc acuacacggg cugggggaag     1980

cuguccgcga agcucaucaa cggcauccgg gacgagaagu ccggcaacac cauccuggac     2040

uaccugaucg acgacggggu cagcaaccgc aacuucaugc agcucaucca cgacgacgcc     2100

cugagcuuca agaagaagau caagaaggcc cagaucaucg gcgacaagga caacaucaag     2160

caggucguga agucccugcc cgggagcccc gccaucaaga agggcauccu ccaguccauc     2220

aagaucgugg acgagcuggu gaaggucaug gggcgggagc ccgagagcau cgugguggag     2280

auggcgcgcg agaaccagua caccaaccag ggcaagucca acagccagca gcggcugaag     2340

cgccucgagg agucccugaa gggccugggg agcaagaucc ucaaggagaa cgucccgacc     2400

cgccugucca agaucgacaa caacgcccug cagaacgacc ggcucuaccu guacuaccug     2460

cagaacggca aggacaugua cacgggggag gagcucgaca ucgaccgccu gagcaacuac     2520

gacaucgacc acaucauccc ccaggccuuc cugaaggacg acuccaucga caacaaggug     2580

cucgugagcu ccgccagcaa ccggggcaag agcgacgacg uccccucccu ggagguggug     2640

aagaagcgca agacccugug guaccagcuc cucaagagca agcugaucuc ccagcggaag     2700

uucgacaacc ucaccaaggc cgagcgcggc gggcugagcc aggaggagaa ggcgggcuuc     2760

auccagcggc agcuggucga gacccgccag aucacgaagc acguggcccg gcuccuggac     2820

gagcgcuuca acaacaagaa ggacgagaac aaccggaccc ugcgcaccgu gaagaucauc     2880

acccucaagu ccagccuggu cucccaguuc cggaaggacu ucgagcugua caaggugcgc     2940

gagaucaacg acuuccacca cgcccacgac gccuaccuca acgccguggu cgcgagcgcc     3000

cugcugaaga aguaccccaa gcucgagccg gaguucgugu acggggacua ccccaaguac     3060

aacuccuucc gggagcgcaa gagcgccacc gagaaggugu acuucuacuc caacaucaug     3120

aacaucuuca agaaguccau cccccuggcc gacggcacgg ucaucgaccg gccccugauc     3180

gaggugaacg aggagaccgg ggagagcgug uggaacaagg ucgccgaccu caacaccgug     3240

cgcaaggugc ugagcuacuc ccaggucaac aucgugaaga agguggagga gcagaaccac     3300

ggccuggacc ggggcaagcc gaagggccuc uucaacgcga accugagcuc caagcccaag     3360

cccaacagca aggagaaccu ggucggggcc aaggaguacc ucgaccccaa gaaguacggg     3420

ggcuacgccg ggaucuccaa cagcuucgcc auccugguga agggcaccau cgagaagggc     3480

gcgaagaaga agaucacgaa cgugcuggag uuccagggga ucagcauccu cgaccgcauc     3540

uacuaccgga aggacaagcu gaacuuccug cucgagaagg gcuacaagga caucgagcug     3600

aucaucgagc ugccgaagua cucccucuuc gagcugagcg acgggucccg ccgcaugcug     3660

gccagcaucc ucuccaccaa caacaagcgg ggcgagaucc acaaggggaa ccagaucuuc     3720

aucagccaga aguucgucaa gcugcuguac cacgccaagc gcaucuccag caccuucaac     3780

gagaaccacc ggaaguacgu ggagaaccac aagaaggagu ucgaggagcu guucuacuac     3840

auccuggagu ucaacgagaa cuacgugggc gccaagaaga acggcgagcu gcucaagucc     3900

gccuuccaga gcuggcagaa ccacuccauc gacgagcugu gcagcuccuu caucgggccc     3960

accggcagcg agcgcaaggg gcuguucgag cucacguccc ggggcggggc ggccgacuuc     4020

gaguuccugg gcgucaagau cccccgcuac cgggacuaca ccccgagcuc ccugcucaag     4080

gacgccaccc ugauccacca gagcgugacc ggccuguacg agacgcgcau cgaccugggg     4140

aagcucggcg aggacuga                                                   4158


<210>  2063
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2063
augcagaccu acuucggcau gaagaagauc cuggggcucg accugggcac caacagcauc       60

gggugggccc uggugaacga ggccaacgac gacaacgaga aguccagcau caucaagcuc      120

ggcguccgcg ugaacccccu gacgguggac gagcagucca acuucgagaa gggcaagagc      180

aucaccacca acgcggagcg gaccaucaag cgcuccaucc ggcgcagccu gcagcgguac      240

aagcuccgcc gggagaaccu gaucgagauc cugaaggagc acaacuucau cacggacgac      300

accauccucu ccgaggacgg gaacgccagc accuucgaga ccuaccgccu gcgggccaag      360

gccgccuccg agaagaucag ccuggaggag cucggccgcg uccugcugau gaucaacaag      420

aagcgggggu acaaguccag ccgcaaggcg aagaaccagg aggagggcca gcucaucgac      480

gggauggagg uggccaagga gcuguacgag aagaacauca cgcccggcca guucguguuc      540

cagcugcuca agcagggcgg gaaguucauc cccgacuucu accgguccga ccugcaggcc      600

gaguucgaca agaucuggga guuccagaag cuguuccacg ccgagauccu cgucgacgac      660

uucaagaagc agcuggagcg caagggcaag cugaacagcu ccaagaucuu ccucgggcgg      720

uucggcaucu acaccgcgga gaacaagggg aacgacaagc gccugcaggc cuaccagugg      780

cggguggacg cccugaccaa ggagcucacc aaggaggagc uggccuacgu gaucagcgac      840

cugaacggca ucaucuccag cuccagcggc uaccucggga gcaucuccga ccgcagcaag      900

gagcuguacu ucaacaacca gacggucggc caguuccuga uggcccagcu cgaccacagc      960

ccccacacca gccugaagaa ccagguguuc uaccggcagg acuaccugga cgaguucaac     1020

acccucuggg agaaccaggc gcacuaccac aaggugcuga ccuccgaccu caagagcgag     1080

auccgcgacg ucaucaucuu cuaccagcgg cgccugaaga cgcagaaggg gcugaucucc     1140

uucugcgagu ucgagagccg caagaucgag guggagaucg acggcaagaa gaagaucaag     1200

aacguggggu cccgggucug ccccaagagc uccccgaucu uccaggaguu caagaucugg     1260

cagauccuga acaacaucga gcuccuggag aaguccucca aggccaagcg cuuccuggac     1320

caggaggaga aggagcuccu guucaaggag cugagcauca aguccaagau cagcaagucc     1380

gacgcccuca agcugcuguu caagaacagc cgggagcugg accucaacua caaggagauc     1440

gagggcaacc gcacccaguc cgcccuguuc gacgccuaca gcaagaucau cgagcugagc     1500

ggccacgggg aguacgacuu cuccaagaug gaggcgagca acaucaucga gcucgugcag     1560

gagguguuca ccggccugaa cuacaacacc gagauccugu acuucgacuc cagccucgag     1620

gggaaggacu ucgagaacca gcccauguac cggcuguggc accuccugua cuccuucgag     1680

ggcgacgaca gcaagacggg gaacgagaag cugaucaaca agcuguccga gcucuacggc     1740

uucgagaaga aguacagcau cguccuggcc aacguguccu uccaggagga cuacggcagc     1800

cuguccacca aggccauccg caagauccac cccuaccuca aggaggggau ccaguacgac     1860

guggccugcg aguucgccgg cuaccggcac agcaagucca gccugaccaa ggaggagauc     1920

gagaacaaga uccugaagga ccagcucgag auccugccca agaacucccu gcgcaacccg     1980

gucguggaga agauccucaa ccagaugauc aacgugguca acgcgcugaa cgagagcuac     2040

gggaagcccg acgagauccg gaucgagcug gcccgcgagc ucaagaaguc cgccaaggag     2100

cgggaggagc ugaccgccag caucggcgag acgaccauca agcacgagaa guaccgcgcc     2160

auccuggaga acgaguucgg gaucagccac gugucccgga acgacaucau ccgcuacaag     2220

cucuaccugg agcuggagaa ccggggcuac aagacccucu acagcaacac cuacgucucc     2280

caggagaagc uguucagcaa ggaguucgac aucgaccaca ucauccccca guccugccug     2340

uucgacgaca gcuucuccaa caagacgcuc gagagcaagu ccgugaacau cgagaagggc     2400

aacgacaccg cguucgacua cguguucgcc aaguacgggg aggccggccu gagcgacuac     2460

aucauccaca ucgaggagcu guucaaggac gggaagaucu ccaagaccaa gcucaacaag     2520

cugaagauga agggcaacga gauccccagc gacuucaucg cccgcgacau ccgggacacc     2580

caguacaucg cgcgcaucgc caagaacauc cuggaggaga cgguccggaa cguggugucc     2640

accagcggcu ccaucaccga ccgccuccgc gaggacuggc agcugaucga cgucaugcag     2700

gagcugaacu gggagaagua cagcgcccuc gggcugaccg agaucuacga gaaccgggag     2760

ggccacacga uccgccggau caaggacugg accaagcgca acgaccaccg gcaccacgcc     2820

auggacgccc ugaccaucgc guucaccaag cgcucccaca uccaguaccu caacaaccug     2880

aacgcccgga gcgacaaguc cgggagcauc uacggcaucg agaccaagga gcuguaccgc     2940

gacgaccacg ggaagcucaa guucaagccg cccauccccc ugaacgaguu ccgggccgag     3000

gccaagaagc accuggagaa cacgcucauc agcaucaagg ccaagaacaa gguggcgacc     3060

aucaacauca acaccaccuc cacgaaggag ggcaagaaca agaagcgcca gcugacgccc     3120

cggggccagc ugcaccucga gaccaucuac gggagcaucc agcaguacgu gaccaagcag     3180

gagaaggucg gcgccuccuu caacgaggag cagaucagca agguggccaa gcagaaguac     3240

cgccaggccc ugcugaaccg gcuccgcgag uucgacaacg acccgaagaa ggccuucacc     3300

gggaacaacu ccuucgagaa gaaccccauc uaccuggacg ugcugcagac ccagaagguc     3360

cccgacaagg uggagaccgu gagcaaggag aucaucuaca ccauccggaa ggacaucacg     3420

cccgagcuca agaucgagaa ggucaucgac aagaagauca agaccauccu gcagucccgc     3480

cugaacgagu acagcggcga cuccaagcgg gcguucagca accucaagga gaacccgauc     3540

uggcugaacc aggagaaggg gaucgagauc aagcgcguga ccaucuccgg caucagcaac     3600

gccguggcca uccacgacaa gaaggacaag aacggcaacc ugauccucga caacgagggg     3660

uccacccagg ccgucgacuu cgugaacacg ggcaacaacc accacguggc gaucuacaag     3720

gacgagcggg ggaaccugca cgagagcguc guguccuucu ucgaggccug cacccgcgug     3780

aaccugggcg ggcccgucau cuucaagaac uacaacaaga gcgacggcug ggaguuccuc     3840

uucuccauga agcagaacga guacuucgug uuccccaacg agaagaccgg cuucaacccc     3900

aacgagaucg accugcugga cccggacaac uaccggcuca ucagccccaa ccuguuccgc     3960

gugcagaaga ucuccagcaa gaacuacguc uucaaccacc accuggagac cagcgccauc     4020

acgggggaga cccucaagaa caagaagcag cuguccagcg ugaccuacca cuucauccag     4080

ucccugccgc ccaucaagga caucgugaag guccgcauca acaacaucgg ccagaucgug     4140

uggguggggg aguacuga                                                   4158


<210>  2064
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2064
auggacgagc ugcagaacuu cgagaagggc aagagcauca ccaccaacgc cgaccgcacg       60

gccgggcggu ccaugcgccg gaaccuccag cgcuacaagc ugcggcgcga gaaccugauc      120

gaggugcuca aggaguacaa cuucaucacc gacgagauga uccugagcga gaacggcaac      180

cggaccaccu ucgagacgua ccgccugcgg gcgaaggccg ccgucgagga gaucucccuc      240

gagcaguucg cccgcgugcu gcugaugauc aacaagaagc ggggguacaa gagcucccgc      300

aaggccaagg gcgcggagaa cgggacccuc aucgacggca uggagguggc caagaagaug      360

uacgacgagg gcaucacccc cggggagcug ugccugcagc uccugaccgc cggcaagcgg      420

uacuucccgg acuucuaccg cagcgaccug caggccgagu ucgaccggau cuggaacuuc      480

cagaagcagu ucuaccccga cucccucauc gacaagguca aggacgaggu gcgcgggaag      540

aacaagagcc agacgugggc gauccuggcc aaguacuucg uguggaagga ggucgagaac      600

uccuggaacg aggaggaggc ccagacccgg cgcguggaga aggaguaccg gcuggugggc      660

aucaagcgcg gggucaagcg ggaggagcuc aagcuggaga acuuccagug gcgcgugaag      720

gcccugagcg agcgcaugaa ccccgaggag cucgccaucg ugcugcagga gaucaacgag      780

cagaucucca acagcuccgg cuaccugggc gcgaucagcg accgguccaa ggagcucuac      840

uucaaccgcc agaccgucgg gcaguaccag auggccgugc uggacaacaa ccccaacauc      900

ggccugcgga acaugguguu cuaccgccag ggguaccucg gcgaguucaa caccaucugg      960

gagaagcagg ccgaguucca caaggagcug acggaggacc ugaagaagga gauccgggac     1020

aucgucaucu ucuaccagcg ccggcucaag agccagaagg gccugaucaa caucugcgag     1080

uucgagcgcc ggcagaucga gguggagauc gacgggaaga agaagaucaa gaccaucggc     1140

ucccgcguga ucccgcggag cagcccccug uuccaggagu ucaagaucug gcagacccuc     1200

aacgacaucg aggucagcgu gcugggggug aagaacaagc gcaagaagca ggacgacaac     1260

agcaccacgc ugcucgacuc cgccgagaac aucgacagcc ugaagcugaa cgucucccgg     1320

ccccucgacg ccgacgagaa gucccugcug gcgaaggagc uguucauccg cgacaagcuc     1380

accaaguccg acgugcugaa gcuccuguuc aacaaccccc agaaccugaa ccucaacuuc     1440

aagaacaucg acggcaaccg gaccgggagc gcccuguacc aggccuucuc caagauccug     1500

gagaucagcg gccacgaguc caucaacuuc aagaaguccg ccgacgagau cguggagcag     1560

gucaagacca ucuucagcgc ccugggcugg aacacggagg ugcucugcuu cgacagcgag     1620

aaggagcugg acaagcagcc guacuucaag cuguggcacc uccuguacuc cuucgagggg     1680

gacagcaccc ccaccggcga cgggaaccug auccagaaga ucgcggaccu cugcggcuuc     1740

gagaaggacu acgccuccau ccuggccaac aucaccuucc aggaggacua cgggagccug     1800

uccacgaagg ccauccgcaa gauccucccc caccugaagg acggcaacca guacgacguc     1860

gccugcgagu acgcgggcua caagcacagc aaguccagcc ugaagaagga ggagaucaag     1920

aacaaggugc ucaaggacaa gcuggagcug cuccccaaga acagccugcg gaacccggug     1980

gucgagaaga uccugaacca gauggugaac gugaucaacg ccaucaucac caccuacggg     2040

aagcccgacg agauccgcau cgagcucgcc cgggagcuga agaagaaggc caagaagcgc     2100

gaggagcuga ccaagacgau cgucgacacc accaagaccc acgagggcau ccgcgcgauc     2160

cuccagaacg acuucgggau gucccacgug agccggaacg ccaucauccg cuacaagcug     2220

uacgaggagc ugaaggacaa cggcuacaag acgcucuacu ccaacaccua cauccccaag     2280

gagaagcugu ucagcaagga cuucgacauc gagcacauca ucccccaggc ccggcuguuc     2340

gacgacuccu ucagcaacaa gacccucgag gugaagucca ucaacaucga gaaggggagc     2400

aagaccgccu acgacuucgu cgaggagaag uacggcgagc agggccugca ggaguaccug     2460

aaccgcugcg agguccucuu ccgggacaag aagacgaagc ugcgcaagcu gaagaugcag     2520

gaguccgaga ucccggaggg guucaucgac cgggaccucc gcaacaccca guacaucgcc     2580

aagaaggcgc ugagcaugcu gaacgagauc ugccggcgcg ugguggccac caccggcgcc     2640

aucaccgacg agcuccggga ggacuggcag cugguggacg ugaugaagga gcugaacugg     2700

gagaaguaca agguccucgg gcugguggag uacuucgagg acaacgacgg ccgccugauc     2760

ggccggauca aggacuggac gaagcgcaac gaccaccggc accacgccau ggacgcccuc     2820

accguggcgu ucaccaagga cgucuucauc caguacuuca acaacaagaa cgccucccug     2880

aagcccgaca ccaacgaggc cgccauccgc aacaaguacu uccagaacgg gaaggccauc     2940

ccgcccauga gccuggacga guuccgggcg gaggccaaga agcaccucaa cgacacgcug     3000

aucuccauca agaccaagaa caagguggug accaucaacc ugaaccgcac caagaagaag     3060

aacggcauca acaagaagca gcagcagacg ccccgggggc agcuccaccu ggagaccguc     3120

uacggcagcc acaagcagua cguggugaag gaggagaagg ucaacgccuc cuucgacgcc     3180

gcgaagaucg ccaccgugaa caagcccgcc uaccgcgacg cccugcuccg gcgccugcag     3240

gagaacggga acgacagcaa gaaggccuuc accggcaaga acucccugga caagcagccc     3300

guguggcucg acgcgaagca gaacgagugc guccccgaga aggugaagac ggugaugcug     3360

gagaccgucu acaccgugcg caagccgauc gacgagaagc ugaacgugga caaggucgug     3420

gacgcccgga uccgcaagcu ccugcaggac cggaucaagg aguacggcaa cgccaagagc     3480

gccuucgcca accuggacga gaaccccauc uggcucaaca aggagaaggg gaucaccauc     3540

aagcgcguga gcgucuccgg caucagcaag gcgcaguccc ugcacgagaa gaaggacaag     3600

gacgggaacu ucauccugga caaggacagc cggcgcaucc ccguggacuu cgugaacacg     3660

ggcaacaacc accacgucgc caucuaccgg aagcccgugc ucgacaagaa ggggcaggcc     3720

guguucgacg agaacggcga gcugaaguuc gagcuggagg aggucguggu guccuucuuc     3780

gacgccguca cccgcgugaa ccucagccag ccgaucaucg acaaggacua caagcggucc     3840

gagggcugga aguuccuguu cagcaugaag cagaacgagu acuucguguu ccccaacgag     3900

aagaccgggu ucgaccccaa ggagaucgac cugcucaacc cggagaacua cgcccugauc     3960

ucccccaacc uguuccgcgu ccagaagauc gcgagcaagg acuacguguu ccggcaccac     4020

cucgagaccg uggucgccga ccgcaaggag cugcaggaga ucacguggaa gcggaucucc     4080

agccugucca uccucaaccg caucaucaag gugcggguca accacaucgg ccagaucgug     4140

augaucgggg aguacuga                                                   4158


<210>  2065
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2065
augaccaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucacggacg acuacaaggu gccgagcaag aagaugaagg ugcucgggaa caccuccaag      120

aaguacauca agaagaaccu gcugggcgcc cuccuguucg acagcgggau caccgcggag      180

ggccgccggc ugaagcgcac cgcccggcgc cgguacacgc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucuc caccgagaug gccacccugg acgacgccuu cuuccagcgc      300

cucgacgaca gcuuccuggu ccccgacgac aagcgggacu ccaaguaccc caucuucggc      360

aaccuggugg aggagaaggc cuaccacgac gaguucccca ccaucuacca ccuccgcaag      420

uaccuggcgg acagcacgaa gaaggccgac cugcggcucg uguaccuggc ccuggcccac      480

augaucaagu accgcgggca cuuccucauc gagggcgagu ucaacuccaa gaacaacgac      540

auccagaaga acuuccagga cuuccuggac accuacaacg cgaucuucga gagcgaccug      600

ucccucgaga acagcaagca gcuggaggag aucgucaagg acaagaucuc caagagcgcc      660

aagaaggacc ggauccugaa gcuguucccg ggggagaaga acuccggcau cuucagcgag      720

uuccugaagc ugaucguggg gaaccaggcc gacuucaaga aguacuucaa ccucgacgag      780

aaggcgcccc ugcacuucuc caaggagagc uacgacgagg accuggagac ccuccugggc      840

uacaucggcg acgacuacuc cgacguguuc cugaaggcca agaagcucua cgacgccauc      900

cugcugagcg ggauccucac cgucacggac aacggcaccg aggcgccccu gagcuccgcc      960

augaucaugc gcuacaagga gcacgaggag gaccuggccc uccugaaggc cuacauccgg     1020

aacaucagcc uggagaccua caacgaggug uucaaggacg acaccaagaa cggguacgcc     1080

ggcuacaucg acggcaagac gucccaggag gacuucuacg ugcaccucaa gaagcugcug     1140

gucgggcucg agggcgccga cuacuuccug gagaagaucg accgcgagga cuuccugcgg     1200

aagcagcgca ccuucgacaa cgggagcauc cccuaccaga uccaccucca ggagaugcgg     1260

gccauccugg acaagcaggc caaguucuac ccguuccugg ccaagaacaa ggagcgcauc     1320

gagaagaucc ugaccuuccg cauccccuac uacgugggcc cccucgcgcg ggggaacucc     1380

gacuucgccu ggagcauccg caagcggaac gagaagauca cccccuggaa cuucgaggac     1440

gugaucgaca aggaguccag cgccgaggcc uucaucaacc gcaugacguc cuucgaccug     1500

uaccugccgg aggagaaggu ccuccccaag cacagccugc uguacgagac cuucaacgug     1560

uacaacgagc ucaccaaggu gcgguucauc gccgagucca ugcgcgacua ccaguuccug     1620

gacagcaagc agaagaagga caucguccgg cuguacuuca agaacaagcg ccgggugacc     1680

gacaaggaca ucaucgagua ccuccacgcg aucgacggcu acgacggcau cgagcugaag     1740

gggaucgaga agcaguucaa cuccagccug uccacguacc acgaccuccu gaacaucauc     1800

aacgacaagg aguuccugga cgacagcucc aacgaggcca ucaucgagga gaucauccac     1860

acccucacca ucuucgagga ccgcgagaug aucaagcagc ggcugagcaa guucgagaac     1920

aucuucgaca aguccgugcu gaagaagcuc agccgccggc acuacaccgg cugggggaag     1980

cugagcgcca agcugaucaa cggcauccgc gacgagaagu ccgggaacac gauccucgac     2040

uaccugaucg acgacggcgu cagcaaccgg aacuucaugc agcugaucca cgacgacgcc     2100

cucuccuuca agaagaagau caagaaggcg cagaucaucg gcgacaagga caacaucaag     2160

caggucgugc aggcccugcc cgggcgcccc gccaucaaga agggcauccu gcagagcauc     2220

aagaucgugg acgagcucgu gaaggucaug gggcggcgcc cggaguccau cgugguggag     2280

auggcccggg agaaccagua caccaaccag ggcaagagca acucccagca gcgccugaag     2340

cgccuggagg agagccucaa ggagcugggg uccaagaucc ugaaggagaa cauccccgcc     2400

aagcucagca agaucgacaa caacgcgcug cagaacgacc ggcuguaccu cuacuaccug     2460

cagaacggca aggacaugua caccggcgag gagcuggaca ucgaccgccu cuccaacuac     2520

gacaucgacc acaucauccc ccaggccuuc cugaaggaca acagcaucga caacaagguc     2580

cuggugucca gcgccuccaa ccgggggaag uccgacgacg ugcccagccu cgaggucgug     2640

aagaagcgca agacccugug guaccagcug cucaagagca agcugaucag ccagcggaag     2700

uucgacaacc ugacgaaggc cgagcgcggc gggcucuccc cggaggccaa ggcgggcuuc     2760

auccagcggc agcuggugga gacccgccag aucaccaagc acgucgcccg gcugcucgac     2820

gagcgcuuca acaacaagaa ggacgaggac aacaagaccc ugcggacggu gaagaucauc     2880

acccucaaga gcucccucgu gagccaguuc cgcaaggacu ucgagcugua caagguccgg     2940

gagaucaacg acuuccacca cgcccacgac gccuaccuga acgccguggu ggcguccgcc     3000

cuccugaaga aguaccccaa gcuggagccc gaguucgucu acggcgacua ccccaaguac     3060

aacagcuucc gcgagcggaa guccgccacc gagaaggugu acuucuacag caacaucaug     3120

aacaucuuca agaaguccau cagccucgcc gacgggaccg ugaucgagcg cccgcugauc     3180

gaggucaacg aggagaccgg cgaguccgug uggaacaagg uggcggaccu gaacacgguc     3240

cggaaggugc ucagcuacuc ccaggugaac aucgucaaga agguggagga gcagaaccac     3300

gggcuggacc gcggcaagcc caaggggcug uucaacgcca accucagcuc caagcccaag     3360

cccaacagca aggagaaccu ggugggcgcc aaggaguacc uggacccgaa gaaguacggc     3420

ggguacgccg gcaucuccaa cagcuucgcc guccucguga aggggaccau cgagaagggc     3480

gcgaagaaga agaucaccaa cauccuggag uuccagggga ucuccauccu ggaccggauc     3540

accuaccgca aggacaaguu caacuuccuc cuggagaagg gcuacaagga caucgaccug     3600

aucaucgagc uccccaagua cagccuguuc gagcugagcg acggcucccg gcgcaugcuc     3660

gccagcaucc uguccacgaa caacaagcgc ggggagaucc acaagggcaa ccagaucuuc     3720

aucagccaga aguacgugaa gcugcucuac cacgccaagc ggaucuccaa caccuucaac     3780

gacaaccacc gcaaguacgu cgagaaccac aagaaggagu ucgaggagcu guucuacuac     3840

auccuggagu ucaacgagaa cuacgugggg gccaagaaga acggcgagcu ccucaagagc     3900

gccuuccagu ccuggcagaa ccacagcauc gacgagcugu gcuccagcuu caucgggccc     3960

accggcuccg agcggaaggg ccuguucgag cugaccagcc gcgggggcgc ggccgacuuc     4020

gacuuccugg gggugaagau cccgcgguac cgcgacuaca cgcccuccag ccuccugaag     4080

gacgccaccc ugauccacca guccgucacc ggccucuacg agacccggau cgaccugggg     4140

aagcugggcg aggacuga                                                   4158


<210>  2066
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2066
augaagaaca agaccuggua ccugggccuc ggggugggcc ccaccagcau cgggugggcc       60

gccacggacu ccaacuacaa gcugcugcgc aagaacaaga agcggcucug gggcguccgc      120

cuguucgagg aggcgaagac cgccguggac cggcgcaccu uccgggccga gcgccggcgc      180

cuggcccggc gcaaguggcg gaucagccuc cuggaggagc uguucgccaa ggaggugucc      240

aagaacgacc cgaacuucuu ccuccgccug aaggagagca aguaccacuu cgaggacaag      300

ucccugauga acgaguacau ccucuucaac gacaaggacu acaccgacaa ggacuacuac      360

aaggaguacc ccacgaucua ccaccuccgg cugagccuga ccaccgagga gaaccccgac      420

auccgcaagg ucuaccuggc ggugcacucc augcugaaga accgggggca cuuccuccug      480

cagggccaga gcuucaacga cuccaacauc gacagcguga ucaaggagcu gcucgagcug      540

gagaccguca agaucaacuu cgaggugacg gacgagguga uccacucccu gaagaagauc      600

gcccucgaga agaagaaccu gagcgacaag aacaacgacg ucaagaagcu guaccccaag      660

gagaagcagc uccaggagau cuucaagcug guguucggcg ggaagaccuc ccuggacaag      720

cucuacgaca ucgaggagua caaggagcug gacaacagcg ugaaguccau cagcuucaag      780

gagaaggucu acgaggaggu gcgcccggac uacgagcaga uccuguccaa cuacaucgag      840

cuccuggacc uggccaagcu cguguacgac agcaucgucc uggccgacau caagaaggag      900

ggcaagacca ucuccgagag caagguggag cuguacaaca agcacaagga cgaccucgcg      960

aaccugaaga agcuggugaa gaucgacagc aagcucuccg aggacaagaa gaaggagguc     1020

uacuucacca ucuucaagga ggacaaggag aaggggacga acuacgugaa cuacacccac     1080

cggagccagg agggcaaguc cugcagcuac gaggacuuca agaaguuccu gaagaaggag     1140

cuggagaagc ucgaggacuc ccccaccaag accacgaucc uggaggagcu ggaccucgag     1200

accuuccugc cccugcagcg caccaaggac aacagcgugg uccccuacca gauccacaag     1260

gaggagcugg ugaagauccu cgacaacgcc gccaaguacc acuccuuccu ggccgagaag     1320

gacgacagcg gguacuccac ccgggagaag gugguccagc uguucgaguu ccgcaucccg     1380

uacuacaucg gcccccucaa cgcccaccac aacacggaga acggcgggua cagcugggcg     1440

auccggaaga agggcaugga guccgugccc gucacccccu ggaacuucaa ggacgugauc     1500

gacgagagcg ccuccgccga ggccuucgug aacaaccuga agaacaagug cgccuaccug     1560

agcggggagg acguccuccc gaagaacucc cugcuguaca gcgaguacgc gcuccugaac     1620

gagcugaacg cccuccgcua cgagggcaac cgcaucuccg uggaggcccg ggacgugauc     1680

gucaccaagc uguucaagga gcagggcaag aaggugacca agaagacgau caaggagcug     1740

cucaagaccg agggguucau cgacggcaag ggggagaucg ugggcaucga caacaucguc     1800

aagaacgacc ucaagagcua ccgcgacuuc aagaagaucc uggccgacaa guucgacaag     1860

gagcgggugg agaacaucau ccuguggauc acccuguacg gggagucccg caagcugauc     1920

aaggagaaga ucaccaaggu guacggcgac uucuacacgg ucgacgagau cgagaagaug     1980

agccggcuca ccuacaagga cuggggcauc cugagccgca agcugcucac cgagcuggug     2040

uccgagaagc uguacaacga ggagaccggg gagugccuca acaucaucgg cgccaugcgg     2100

caggggacga uccuguucau ggagcugcuc gcggacaagu ucgacuacau gagccagauc     2160

aagaaccaca acaaggagaa caaggacgac aucaccgaga ucacccccga cauccuggag     2220

aaccugcacg ccucccccgc cgugaagcgc agcaucuggc agaccguccg gaucguggag     2280

gagcucaaga cgaucaucgg cugcgugccc accaagaucu ucaucgagac caccaagcgc     2340

aacaacgccc cguacaaggu ccccacccug aaccggaacg accuggugac gaaguacaag     2400

accauccgcg agcacgagau cuucgagcuc gagaaggagc ugaagaacac ccuggacuuc     2460

acccucaaca agaaccggcu gaucaacgag gagcccucca agcugaaggc cacgaagcuc     2520

uaccuguacu acacccagcu ggggcgcugc auguacaccg gcaagaagau cgaccucggc     2580

gagcuguucg acaacaacaa caaguacgac aucgaccaca ucuaccccca gagcaaggug     2640

aaggacgggu ccuucaacaa caucguccug gugaccaagg cgagcaacga caugaaggcc     2700

gacguguucc cgcucaaguc caaguuccag acgaaggaga acaagaucuu cuggcgguuc     2760

cugaaggaga agaagcugau caccgaggag aaguacaacc gccucguccg gaccgaggag     2820

uucagcaacg gcgagcugac cggguucauc acgcgccagc ugguggagac cucccaggcc     2880

aucaagguga ucagcaacgu ccuccgggag cugaaccccg agaccaccau cugcuacucc     2940

aaggccgaga acgugagcuc cuuccgccag agcuucggca agaucaagga ggcgaaccgc     3000

aaggccgaga acaacgagaa gcugauccgg gugcaggaga ucaacgacua cuacaacgcc     3060

aaggacgccu accucuccau cgucgugggg aacguguacg acgucaaguu cacgcgcaac     3120

gugcacaacu ucaucaagaa caacaaggac gcccggcgcu acagccugaa cggccuguuc     3180

uacaccgacg ugaagaacgc gaacaccauc gccugggaga uggacaagac cguccacgug     3240

guggagaaga cgaugaacaa caacaacauc cucaucaccc ggcgcaccag cgagcagaag     3300

ggcgcccugu acgacgccac ccuguacaag gccaaggucg cggagaaggc caaggagggg     3360

acguacuacc cccucaagac caccgacucc aagaucaagg acgugggcaa guacgggggc     3420

uucaccaaga ucaagaucgc cuacuacagc aucuucgagu acacgcugga cggcaagaag     3480

gggaagcaga agaucacccg gaucgugccc aucccgaucu acaucuccca gaacaucaag     3540

gacgacaagg cccugcucga guacgcccag acccgccugc ccggcaagga ccugaagauc     3600

aaguaccgga agcucugcau cgggagccug gucaagauca acaacuucaa cuacuacauc     3660

ggcgggaaga ccaacaacaa cuucacguuc gacuccgcgg uccaggugau ccuggacaag     3720

gacagcgagu ccuacaucaa ggagcucgcc aaguaccuga acuggaagaa ggagaacaag     3780

gacggcaagc ugugggagag caucaccgag gacaagaaca uccagcucua cgacucccug     3840

gugaagaaga ugaacaccgg caucuucauc cgcaagaccc ccaacaagua cacggagcug     3900

cucacccugg aggugaagaa caaguucauc aacaucgaca ucgacgagca ggucaagcug     3960

cuccuggaga uccugaaccu ccugaccaac aagaaguuca ccagcaagcu gaacgagauc     4020

gggaucaccg ccucccgggg cgugugcagc uucaaccucu ccaaccagag ccaguucucc     4080

aucaucgagc agagcgugac ggggguguac gagaaggaga agcugaucau cgagaagaag     4140

aaggagggca uccuguga                                                   4158


<210>  2067
<211>  4161
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2067
augaaccaga aguacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucaccgacg acuacaaggu gcccgccaag aagaugaagg ugcucgggaa cacggacaag      120

cagagcauca agaagaaccu gcugggcgcg cuccuguucg acuccgggga gaccgccgag      180

gccacccgcc ugaagcggac cgcccgccgg cgcuacacgc ggcgccggaa ccgccuccgg      240

uaccugcagg agaucuucgc cgaggagaug augcaggucg acgagagcuu cuuccagcgc      300

cuggacgacu ccuuccucgu ggacgaggac aagcggggcg agcgccaccc gaucuucggc      360

aacaucgcgg ccgaggugaa guaccacgac gaguucccca ccaucuacca ccuccggaag      420

caccuggccg acaucagcca gaaggccgac cuccgccugg ucuaccuggc gcucgcccac      480

augaucaagu uccgggggca cuuccugauc gagggccagc ugaaggccga gaacaccaac      540

gugcaggccc ucuucaagga cuucguggag gucuacgaca agaccgugga ggagucccac      600

cugagcgaga ucacggugga cgcccugucc auccucaccg agaaggucag caagucccgc      660

cggcuggaga accugaucgc gcacuacccc gccgagaaga agaacacccu cuucgggaac      720

cugaucgccc uguuccucgg ccugcagccc aacuucaaga ccaacuucca gcugagcgag      780

gacgccaagc uccaguucuc caaggacacg uacgaggagg accuggaggg gcugcucggc      840

gaggugggcg acgaguacgc cgaccuguuc gcgagcgcca agaaccugua cgacgccauc      900

cuccuguccg ggauccugac cguggacgac aacagcacca aggccccgcu cuccgccagc      960

auggucaagc gcuacgagga gcaccagaag gaccugaaga aguucgagga cuucaucaag     1020

gugaacgcgc uggaccagua caacgccauc uucaaggaca agaacaagaa gggcuacgcc     1080

ggguacaucg aguccggcgu gaagcaggac gaguucuaca aguaccucaa gggcauccug     1140

cugcagauca acgggagcgg cgacuuccuc gacaagaucg accgggagga cuuccugcgc     1200

aagcagcgga ccuucgacaa cgggagcauc ccccaccaga uccaccucca ggagaugcac     1260

gccauccugc gccgccaggg cgagcacuac cccuuccuga aggagaacca ggacaagauc     1320

gagaagaucc ugacguuccg gauccccuac uacgucgggc cgcuggcccg caagggcucc     1380

cgguucgcgu gggccgagua caaggccgac gagaagauca cccccuggaa cuucgacgac     1440

auccucgaca aggagaaguc cgccgagaag uucaucaccc gcaugacccu gaacgaccug     1500

uaccuccccg aggagaaggu gcugcccaag cacagccugc ucuacgagac guucaccgug     1560

uacaacgagc ugaccaaggu caaguacgug aacgagcagg gcgaggcgaa guucuucgac     1620

gccaacauga agcaggagau cuucgaccac guguucaagg agaaccggaa ggucaccaag     1680

gacaagcugc ucaacuaccu gaacaaggag uucgaggagu uccgcaucgu gaaccugacg     1740

gggcucgaca aggagaacaa ggucuucaac agcucccugg gcaccuacca cgaccugcgg     1800

aagauccuca acaagagcuu ccuggacaac aaggagaacg cccagaucau cgaggacauc     1860

auccagaccc ugacccucuu cgaggaccgc gagaugaucc ggcagcgccu gcagaaguac     1920

uccgacaucu ucacgaaggc ccagcugaag aagcucgagc ggcgccacua caccgggugg     1980

ggccggcuga gcuacaagcu gaucaacggg auccgcaaca aggagaacaa gaagaccauc     2040

cucgacuacc ugaucgacga cggcuacgcc aaccggaacu ucaugcagcu gaucaacgac     2100

gacgcgcucu ccuucaagga ggagaucgcc aaggcccaga ucaucggcga cguggacgac     2160

aucgccaacg ugguccacga ccugccgggg agccccgcca ucaagaaggg cauccugcag     2220

uccgugaaga ucguggacga gcucgucaag gugauggggc acaaccccgc gaacaucauc     2280

aucgagaugg cccgcgagaa ccagaccacg gaccggggcc gccggaacag ccagcagcgc     2340

cugaagcugc uccaggacuc ccugaagaac cuggacaacc ccgugaacau caagaacguc     2400

gagaaccagc agcuccagaa cgaccgccug uuccuguacu acauccagaa cgggaaggac     2460

auguacaccg gcgagacccu cgacaucaac aaccugagcc aguacgacau cgaccacauc     2520

aucccgcagg ccuacaucaa ggacgacucc uucgacaacc gggugcugac cagcuccagc     2580

gagaaccgcg gcaaguccga caacgugccc agcaucgagg ucgugugcgc ccggaaggcc     2640

gacuggaugc gccuccggaa ggcggggcug aucagccagc gcaaguucga caaccugacg     2700

aaggccgagc ggggcgggcu caccgagaac gacaaggccg gcuucaucaa gcgccagcug     2760

guggagaccc ggcagaucac caagcacguc gcccaggugc uggacgcgcg cuucaacgcc     2820

aagcacgacg agaacaagaa ggugauccgg gacgucaaga ucaucacccu caaguccaac     2880

cuggugagcc aguuccgcaa ggacuucaag uucuacaagg ugcgggagau caacgacuac     2940

caccacgccc acgacgccua ccugaacgcc gucaucggca cggcgcuccu gaagaaguac     3000

cccaagcugg ccuccgaguu cguguacggg gaguucaaga aguacgacgu gcgcaaguuc     3060

aucgccaaga gcgacaagga gaucggcaag gccaccgcca aguacuucuu cuacuccaac     3120

cucaugaacu ucuucaagaa ggaggucaag uucgcggacg ggaccguggu ggagcggccc     3180

gacaucgaga ccagcgagga cggcgagauc gccuggaaca agcagacgga cuucaagauc     3240

guccgcaagg ugcuguccua cccccaggug aacaucguca agaagaccga ggugcagacc     3300

cacgggcugg accggggcaa gccgcgcggc cucuucaacg ccaaccccag ccccaagccg     3360

aagcccgacu ccagcgagaa ccuggugggg gucaagcgga accuggaccc caagaaguac     3420

ggcggguacg ccggcaucuc caacagcuac gccgugcucg ugaaggcgau caucgagaag     3480

ggggucaaga agaaggagac cauggugcug gaguuccagg gcaucuccau ccuggaccgc     3540

aucacguucg agaaggacaa gcgggccuuc cuccugggca agggguacaa ggacaucaag     3600

aagaucaucg agcugcccaa guacagccuc uucgagcuga aggacggcuc ccgccgcaug     3660

cuggccagca uccucuccac caacaacaag cgcggggaga uccacaaggg caacgagcug     3720

uucgugccgc agaaguucac cacccugcuc uaccacgcca agcggaucaa caaccccauc     3780

aacaaggacc acaucgagua cgucaagaag caccgggacg acuucaagga gcugcugaac     3840

uacgugcucg aguucaacga gaaguacgug ggggcgacga agaacggcga gcgccugaag     3900

gaggccgucg ccgacuucga cagcaagagc aacgaggaga ucugcaccuc cuuccugggc     3960

gccgugaaca gcaagaacgc cgggcuguuc gagcugaccu cccugggcag cgcguccgac     4020

uucgaguucc ucggggugaa gaucccccgg uaccgcgacu acaccccgag cucccugcug     4080

aaggacagca cgcucaucca ccaguccauc accggccugu acgagacccg gaucgaccug     4140

agcaagcucg gggaggacug a                                               4161


<210>  2068
<211>  4161
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2068
augaccaaga ucaccgagga cuacaucgug ggccuggaca ucgggacgaa cagcugcggc       60

ugggucgcca uggacuccaa gaacaccauc cuccgcaugc acgggaagac cgccaucggc      120

agccaccugu ucgacgcggg gaacuccgcc gccgaccggc gcgccuuccg gaccacgcgc      180

cggcgcauca agcggcgcaa guggcggcug aagcuccugg aggagaucuu cgacagccac      240

cuggccaagg uggaccccua cuucuucgcg cgccucaagg aguccggccu gagcccgcug      300

gacacccgga agaacguguc cagcaucauc uaccccaccu cccucgagga caagaaguuc      360

uacgucgaca accccaccau cuaccaccuc cgcuacaagc ugaugacgga ggaccggaag      420

uucgaccugc gcgaggugua ccuggccauc caccacaucg ugaaguaccg gggcaacuuc      480

cuguacaaca cccccgucaa ggccuucgag gccagcaaga ucgacgugaa ggggucccuc      540

aagaagcuga acgagcugua cgagggccuc gacagcgagu ucaacgugga gcuggacucc      600

aacaacgcgc uggagaucga gaacaucauc cgcgacaaga agaucuucaa gaucaacaag      660

gucaaggaga ucaaccagcu ccugagccug aagaccgagg acaaggagcg gaccaagcuc      720

augaaggacg uguccaagca gaucgugaac gccauccugg gguacaagac gaaguucgag      780

accauccugc ucaaggacgu cagcaaggac gaggccgacg acugggaguu caagcugacc      840

gacguggacg ccgacaacaa guucgagaac cugaucggcg accucaacga gaacgagcag      900

gagauccugc uggugauccg caagcucgcc aacgcgauca cccuguccaa caucgucgag      960

gaggggaaga cgcugagcga guccaugaag gacaaguaca agaagcacag cgacgaccuc     1020

aagcugcuga agcaggucau cagcgaccac ccggaccggg acaaggccaa gaagcucgcc     1080

cuggccuacg accuguacgu gaacaaccgc cacggcaaga uccuccaggc caaggacauc     1140

cuggagaaga agaagacccu guccaaggag gacuucuaca aggagaucaa gaagaaccuc     1200

gacgacagca aggaguccca ggagauccug gacgcgauca gccuggacuc cuucaugccc     1260

aagcagcgga ccaacgagaa cggcgugauc cccuaccagc uccaccagcu ggagcuggac     1320

aagaucaucg agaaccaggg gaaguacuac cccuuccuga aggaggugaa cccggugagc     1380

ucccaccuca agcaggcccc cuacaagcug gacgagcuga uccgcuuccg cguccccuac     1440

uacgugggcc cccucaucag cccgaacgag uccaccaaga acaagcagac gaagaagaac     1500

cagaacuucg ccuggaugau ccggaaggag caggggcaga ucacccccug gaacuucgac     1560

cagaaggugg accgcauggc cagcgccaac aaguucauca agcggaugac caccaucgac     1620

acguaccugc ugggcgagga cguccucccc gcgaacuccc ugcuguacca gaaguucacc     1680

gugcucaacg agcugaacaa cgucaagauc aacggcaagc gcaucagcgu gccccugaag     1740

caggagcucu acaacaaccu guucaagaag aacuccaccg ugaccacgaa caagcucaag     1800

agcuaccuga aggagaacua caagcugauc aaggucgaga ucaaggggcu guccgacgag     1860

aagaaguuca acagcggccu caccaccuac aacaagcuga agaaccugaa gaucuucgac     1920

cagcagaucg acgacccgaa cuacaacaag gacuucgagc ggaucaucga gugguccacc     1980

aucuucgagg acaaggccau cuacaaggag aagcucaaga cgaucgacug gcugagcgac     2040

cgccagaucg acaagcuguc caagauccgg augcaggggu ggggccagcu cagcaagaag     2100

cugcugagcc agcucaccga caacaacggg cagaccauca ucgagcagcu gugggacucc     2160

cagaacaacu ucaugcagau cgugaaccag gccgacuuca aggacgccau cgccguggcg     2220

aaccagaacc ugcucgucga caccagcgug gaggacaucc ugaacgaggc cuacacgucc     2280

cccgccaaca agaaggccau ccgccaggug gucaaggugg uggacgacau cgucaaggcg     2340

accagcggca aggugcccaa gcagaucgcc aucgaguuca cccgggacgc cgacgacaac     2400

gccaagaucu cccagagccg cgccaacaag cugcggaagg uguacaagga gauguccaac     2460

gagcucgcga gcgagaccau ccgcaacgag cuggagcggg ucgccaagga ccagaagcug     2520

cucaaggaca agcacuaccu guacuucaug cagcugggcc gcgacgccua caccggggag     2580

cucaucgaca ucgacaaccu ggagcaguac gacaucgacc acgugcugcc ccaguccuuc     2640

aucaaggacg acagccucga gaaccgggug cuggucaaga aggccgugaa caacggcaag     2700

uccgacaacg ugccggucaa gcuguacggg aaccgcaugg ccacggaccu cggcaugacc     2760

auccggcaca ugugggagaa guggaaggag caggggcuga ucaccaagac caaguacaac     2820

aaccugauca ucgaccccga caagaucaac aaguacgaga gcuccggcuu cauccaccgc     2880

cagcucgugg agacgagcca gaucaucaag cuggugucca ccauccugca gagcaaguac     2940

cccgacaccg agaucaucgu cgugaaggcg cgguacaacc acuaccuccg caagcgccug     3000

aaccuguaca agucccggga ggugaacgac uaccaccacg ccaucgacgc cuaccucagc     3060

gccaucugcg gcaaccugcu guaccagguc uaccccuacc uccgcccguu cuucguguac     3120

gggcaguaca agaaguucag cuccgagccc gagaaggaga agaucaucua cgacaagacc     3180

cggcgcuaca acuucaucag ccagaucuuc gagaacaagg acaacgacau caucucccgg     3240

gagacgaaga agaagguguu cgacaagaag gacaucaucg agaagcugaa gcgcgccuac     3300

aacuacaagu acaugcuggu cagccgggag accgagaccc acgaccagga gauguucaag     3360

augaccgugu acccccgccu cucccgggac acgaagaagu cccgcaaccu gauccccaag     3420

aagaaggaca ugccgaccga gaucuacggc ggguacacca acaacagcga cgcguacaug     3480

aucaucgccc ggaucaacaa gaagaagggc cccgaguacc gcguguucgg gguccccaug     3540

cgggagcugg ugaaccuccg caaggccgag aagaacggcc acuacaaggc cuaccugaag     3600

cagguccugg agccggagau cauguacagc aagaacggca agaagaacaa gaccaucucc     3660

agcuucgaga ucgugaaggc gaaggugccc uacaagcagg ucauccucga cggggacaag     3720

aaguucaugc uggccuccag cacguacgug cagaacgcca agcagcugac ccucucccag     3780

uacagcaugc aggccaucac cgacaacugc gagaacgaca ccgaggagga gaaggcccug     3840

guggaggcgu acgacgagau ccugacgaac aucgacaagu accucccccu guucgacauc     3900

aacaaguucc gggagaagcu gcacgccggc cgcgagaagu ucguggaccu cccccuggcc     3960

gagaagaagg agaccauccu gcagguccuc aacgggcugc acgacaacgc cgugaugccg     4020

aagaucaagu cccugggccu cagcacgccc cugggcuuca ugcaguucca caccggggug     4080

ucccugagcg agaacgccaa gcucaucuac caguccccca ccggccuguu cgagaagcgg     4140

cugaagauca gcgaccucug a                                               4161


<210>  2069
<211>  4164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2069
augagcgacu ccaagaaguu cgagggcgag aacgucaaga gcggggacaa gaagaccaac       60

aagaaccacg gcaaggugcu gaaguccagc cccaaggaga uccgcaagaa caccgaguac      120

uucaucgggc ucgacaucgg cacguccagc gugggguggg ccgugaccaa caccgacuac      180

gagauccuga aguucaacgg caagcggaug uggggcuccc gccuguucaa cgaggccagc      240

accgcggagg agcggcgcgg gcaccggguc aaccgccggc gcauccagcg gcgccgggac      300

cgccucaacc ugcuggagga gaucuucucc gaggagaugg ccaaggugga cccggaguuc      360

uucaugcggc ucaaggagag caaguuccac cuggaggaca aggacgagcg cauccugggc      420

gaguccaaca ucaucuucaa ggggagcgag gacggcgaca agaaguacua cgagaaguac      480

cccacgaucu accaccuccg gcacaagcug augcaguccu gcgaggacgu ggacauccgc      540

gagcuguaca uggcccucca ccacaucauc aaguaccggg ggcacuuccu guucaagggc      600

gacuucgagg agaccggcac cagcaucgag aacaucauca ucgagcugcu cgagcugauc      660

aacaacaacc ugggggucaa gaaguucgag uacaacgagu cccucaucaa cgagcugaag      720

aacaucguga gcaacaccuc caacacgauc accaacaaga agaagcugcu cggcgaguuc      780

uucaacaaga aggaguacaa ggcccacaag agccuguuca uccuguccau cggggccagc      840

gugaagcccg ucgaccucuu ccuggaggag gaguacaagg acaucgacga caucaagaac      900

aucagcuuca agaaccugga guacgaccag gugcgcgaca ccuacgaguc caucuuccuc      960

gacaagaucg aguucaucga cguggcgaag cggcuguacg acgccaccau ccugcucagc     1020

aucaagcccg agggcaagac gcuguccgag gccaaggucg acaucuacaa caagcaccac     1080

aacgaccugg agcuccugaa gaaggugcug aagaccgaca aggagaagua cgacagcauc     1140

cucaagcugg acaucaagaa gggcaacaac uacgugaacu acgucaagaa guccaagaac     1200

gggaucagcu gcuccaagga ggaguuccac aacgcccuga agaaggugcu cgugaagauc     1260

gagaagaccg acgacaucaa guacauccug aacgagaucg agcuggagcg cuuccucccg     1320

cugcagcggg ucaaggacaa cagcgugauc cccuaccaga uccaccgcua cgagcugaag     1380

aagauccucg agaacgcguc caagaaguuc accuuccuga acgaggagag cgacggccac     1440

acgaacaucg agaagaucau caagaucaug gaguaccgca uccccuacua cguggggccc     1500

cugaacgacc accacuccgu cuacaacaac ggcuucgccu gggugagccg gaagcgcucc     1560

gggcggauca ccccguggaa cuucgacgag augguggaca aggaggagag cgccgagcgc     1620

uucaucgaga accucaccaa caagugcucc uaccugaucg gcgaggacgu ccugcccaag     1680

gagagccugc ucuacuccga guucagccug cugaacgagc ucaacaacau caaguacgac     1740

ggcaucuccc ugcccaucga caucaagaag uccaucgugg agaagaucuu ccgggucgag     1800

aagaagcgcg ugaccaagaa guacaucaag gaguuccuga agcaggaggg guacagcgac     1860

ggcaaggggg aggugagcgg caucgacauc gacaucaaga gcgaccucaa guccuaccgg     1920

gacaugguca agaucuucgg gacggagauc gacuaccgca uggccgagaa caucaucaag     1980

ugggugaccc uguucggcga cagcaaggac cugcucaaga ccaagaucaa caaggaguac     2040

ggcgagaagg uguccaagga ccagcugaag aaggucugca agcugaagua ccgggacugg     2100

gggcgccuca gcaagaaguu ccugaccggc auccggucca gcgugcuggu gaacgacgcc     2160

acgggggagg gcuacaccau caucgaggcg augcgcaaca ccgacaacaa ccucaugcag     2220

cugcugcgga acgaguacga cuaccucaag aagaucgacc agaucaacau gcugucccgc     2280

aaggacaucg acgagaucag ccacgacauc cuggacgacc ucuacugcuc ccccgccguc     2340

aagcggagcc uguggcagac caucaagauc guggaggaga ucaagaagau cauggggcac     2400

gagccgaaga agguguucgu cgaggugacg cgcaccaaca aggccgacaa gaagaccacc     2460

aucacgcgga agaaccgccu gcucgagcug uacuccgcca ucaacgccga gagccgggac     2520

ugggugaacg agcugaacuc caccgagguc agcaaguuca acuccaagaa gcucuaccug     2580

uacuacaccc agaugggcaa gugcauguac accggcaaga acaucgacau cgagagccug     2640

uucacggaca uguacgacau cgaccacauc uucccccgca gcaagaccaa ggacaacucc     2700

uucgagaacc ucgugcuggu gacccgcaag gcgaacgccg acaagcggga cgucuacccc     2760

aucgacccca agauccagac cgagcgcaag agccuguggg acaugcucca cgacaagaag     2820

cugaucgggg acaagaagua cgaccggcug acccgcacga ccggcuucac cgacgacgag     2880

auguccgggu ucaucgcccg gcagcucgug gagaccagcc aggccuccaa ggccgcggcc     2940

gagaugcuga agcugaucaa caacgacagc gagaucgugu acgucaaggc cgagaacgug     3000

uccgacuucc gccacgagaa cgaguucauc aaggugcggg agaucaacga ccaccaccac     3060

gccaaggacg ccuaccucaa caucgucgug ggcaacgugu acaacgagaa guucacgaag     3120

aacccggcga acuacaucaa gaagggcggg ggcaacaacu acagccugaa cugcaucuuc     3180

aaccgcaacc uggaccggaa cgggaagcgc aucugggacg agaagaaguc caucgacacc     3240

gucaaccgga ugaugaaguc caacgacgug cgcgugaccu ucaagaaccg ggucgacaag     3300

ggccccaucc ucaacaaggu gaccauccac aaggccagcg aguucaugga cgacgggaag     3360

aagaacaaga agggcuuccc caucaagagc uccgagaaga acggcaacaa ccgccugugc     3420

aaccuguaca aguacggggg cuacaaggac cucaacuaca gccacuacac gaucgugcgg     3480

uacaacgucu acgagaagaa ggggcuggac aucaaguacu acaugauccc caucccguac     3540

uaccugugcc ucugcaacau caaccagcac gacuacgugu acgacuacau cuccaaccgc     3600

agcaagaagg acgugaacaa caucgagauc aucgucaacg agcugaagau gggcucccug     3660

aucaacaucg acggguacaa cuacuaccuc ggcggcagca agcccaagac cuucuacgug     3720

gaccccgccu accagaucau ccuggacaag gagaucgagc ggugcaucaa ggagaucgug     3780

aaggccaaga acaacgagua ccgcuucauc aacaccgaca agaucaacaa gaaccugaac     3840

augaagaucu acaaguacau cgucaacaag aagaucaucg ggguguuccg gaaccgcaag     3900

ugggacaagg gcgaguaccu caacuccagc gagaccaucg agaaguucag cgggcugucc     3960

guggagagcc agugcaacgu cauccugcgg cucaucaacg acaugcgcgg cuccaacaag     4020

ggcggggcga acagcaaguu cgacgagcug gagaugacga ucucccgcgc cgagaucccc     4080

cagacccuga gcaacguguc cagcuucaag aucaucaacc aguccaucac cggccucuuc     4140

gagaacgagg ugaccaucgu cuga                                            4164


<210>  2070
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2070
augaccaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucacggacg acuacaaggu gccgagcaag aagaugaagg ugcucgggaa caccuccaag      120

aaguacauca agaagaaccu gcugggcgcc cuccuguucg acagcgggau caccgcggag      180

ggccgccggc ugaagcgcac cgcccggcgc cgguacacgc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucuc caccgagaug gccacccugg acgacgccuu cuuccagcgc      300

cucgacgaca gcuuccuggu ccccgacgac aagcgggacu ccaaguaccc caucuucggc      360

aaccuggugg aggagaaggc cuaccacgac gaguucccca ccaucuacca ccuccgcaag      420

uaccuggcgg acagcacgaa gaaggccgac cugcggcucg uguaccuggc ccuggcccac      480

augaucaagu accgcgggca cuuccucauc gagggcgagu ucaacuccaa gaacaacgac      540

auccagaaga acuuccagga cuuccuggac accuacaacg cgaucuucga gagcgaccug      600

ucccucgaga acagcaagca gcuggaggag aucgucaagg acaagaucuc caagcuggag      660

aagaaggacc ggauccucaa gcuguucccg ggggagaaga acagcggcau cuucuccgag      720

uuccugaagc ucaucguggg gaaccaggcc gacuucaaga aguacuucaa ccuggacgag      780

aaggccagcc ugcacuucuc caaggagagc uacgacgagg accucgagac ccugcugggc      840

uacaucggcg acgacuacuc cgacguguuc cucaaggcca agaagcugua cgacgccauc      900

cugcucagcg ggauccugac cgucacggac aacggcaccg aggcgccccu gagcuccgcc      960

augaucaugc gcuacaagga gcacgaggag gaccucgggc ugcugaaggc cuacauccgg     1020

aacaucagcc ucgagaccua caacgaggug uucaaggaca acuccaagaa cggcuacgcc     1080

ggcuacaucg acgggaagac caaccaggag gacuucuacg uguaccugaa gaaccugcuc     1140

agcaacuucg agggcgccga cuacuuccug gagaagaucg accgcgagga cuuccugcgg     1200

aagcagcgca cguucgacaa cggguccauc cccuaccaga uccaccucca ggagaugcgg     1260

gcgaucaucg acaagcaggc caaguucuac cccuuccugg ccaacaacaa ggagcgcauc     1320

gagaagaucc ugaccuuccg caucccguac uacgucggcc cccuggcccg ggggaacagc     1380

gacuucgccu gguccauccg caagcggaac gagaagauca cccccuggaa cuucgaggac     1440

gugaucgaca aggagagcuc cgcggaggcc uucaucaacc gcaugaccag cuucgaccuc     1500

uaccugcccg aggagaaggu gcugccgaag cacucccucc uguacgagac guucaacguc     1560

uacaacgagc ugaccaaggu gcgguucauc gccgagagca uguccaagua ccaguuccuc     1620

gacagcaagc agaagaagga caucgugcgc cuguacuuca aggacaagcg gaaggucacc     1680

gacaaggaca ucaucgagua ccugcacgcc aucgacggcu acgacggcau cgagcucaag     1740

gggaucgaga agcaguucaa cuccagccug uccaccuacc acgaccugcu caacaucauc     1800

aacgacaagg aguuccugga cgacagcucc aacgaggcca ucaucgagga gaucauccac     1860

acgcugacca ucuucgagga ccgcgagaug aucaagcagc ggcucagcaa guucgagaac     1920

aucuucgaca agagcgugcu gaagaagcug ucccgccggc acuacaccgg cugggggaag     1980

cucagcgcga agcugaucaa cggcauccgc gacgagaagu ccgggaacac cauccuggac     2040

uaccucaucg acgacggcau cagcaaccgg aacuucaugc agcugaucca cgacgacgcc     2100

cuguccuuca agaagaagau ccagaaggcc cagaucaucg gcgacgagga caaggggaac     2160

aucaaggagg uggucaagag ccuccccggc ucccccgcca ucaagaaggg gauccugcag     2220

agcaucaaga ucguggacga gcuggugaag gucaugggcg ggcgcaagcc cgaguccauc     2280

guggucgaga uggcgcggga gaaccaguac acgaaccagg gcaagagcaa cucccagcag     2340

cgccucaagc ggcuggagaa gucccugaag gagcucggca gcaagauccu gaaggagaac     2400

aucccggcca agcugagcaa gaucgacaac aacgcccucc agaacgaccg ccuguaccug     2460

uacuaccucc agaacgggaa ggacauguac accggcgacg accuggacau cgaccggcug     2520

uccaacuacg acaucgacca caucaucccc caggccuucc ucaaggacaa cagcaucgac     2580

aacaaggugc ugguguccag cgccagcaac cgcgggaagu ccgacgacgu ccccagccug     2640

gaggugguga agaagcgcaa gacccucugg uaccagcugc ugaaguccaa gcucaucagc     2700

cagcggaagu ucgacaaccu gaccaaggcg gagcgcggcg gccugucccg ggaggacaag     2760

gccagcuuca uccagcgcca gcucgucgag acgcggcaga ucaccaagca cguggcccgc     2820

cugcuggacg agaaguucaa caacaagaag gacgagaaca accgggccgu gcgcaccguc     2880

aagaucauca cccucaaguc cacgcuggug agccaguucc ggaaggacuu cgagcuguac     2940

aaggugcgcg agaucaacga ccuccaccac gcccacgacg cguaccugaa cgccgucgug     3000

gccuccgccc ugcucaagaa guaccccaag cuggagccgg aguucgugua cggggacuac     3060

cccaaguaca acagcuuccg ggagcgcaag uccgccaccg agaaggucua cuucuacagc     3120

aacaucauga acaucuucaa gaaguccauc agccuggcgg acggcaccgu gaucgagaag     3180

ccccucaucg aggugaacga ggagaccggg gaguccgucu ggaacaagga gagcgaccug     3240

gccaccgugc ggcgcgugcu guccuacccc caggucaacg uggugaagaa ggucgagaag     3300

cagaaccacg gccucgaccg ggggaagccg aagggccugu ucaacgccaa ccugagcucc     3360

aagcccaagc ccaacagcaa cgagaaccuc gugggcgcca aggaguaccu ggaccccaag     3420

aaguacgggg gcuacgcggg gaucagcaac uccuucgccg ugcuggucaa gggcacgauc     3480

gagaaggggg ccaagaagaa gaucaccaac gugcucgagu uccagggcau cagcauccug     3540

gaccgcauca acuaccggaa ggacaagcug aacuuccucc uggagaaggg cuacaaggac     3600

aucgagcuga ucaucgagcu cccgaaguac ucccuguucg agcugagcga cgggucccgc     3660

cggaugcucg ccagcauccu guccaccaac aacaagcgcg gcgagaucca caaggggaac     3720

cagaucuucc ugagccagaa guucgugaag cuccuguacc acgccaagcg gaucuccaac     3780

accaucaacg agaaccaccg caaguacguc gagaaccaca agaaggaguu cgaggagcug     3840

uucuacuaca uccucgaguu caacgagaac uacgugggcg cgaagaagaa cgggaagcug     3900

cugaacagcg ccuuccaguc cuggcagaac cacagcaucg acgagcucug cuccagcuuc     3960

aucggcccca cgggcuccga gcgcaagggg cuguucgagc ugaccagccg gggcuccgcc     4020

gccgacuucg aguuccucgg ggugaagauc ccccgcuacc gggacuacac cccgagcucc     4080

cugcugaagg acgccacccu cauccaccag agcgucacgg gccuguacga gacccgcauc     4140

gaccuggcga agcuggggga gggcuga                                         4167


<210>  2071
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2071
augaccaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucacggaca acuacaaggu gccgagcaag aagaugaagg ugcucgggaa caccuccaag      120

aaguacauca agaagaaccu gcugggcguc cuccuguucg acagcgggau caccgccgag      180

ggccgccggc ugaagcgcac cgcgcggcgc cgguacacgc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucuc caccgagaug gccacccugg acgacgccuu cuuccagcgc      300

cucgacgaca gcuuccuggu gcccgacgac aagcgggacu ccaaguaccc caucuucggc      360

aaccuggugg aggagaaggu cuaccacgac gaguucccca ccaucuacca ccuccgcaag      420

uaccuggccg acagcacgaa gaaggccgac cugcggcucg uguaccuggc gcuggcccac      480

augaucaagu accgcgggca cuuccucauc gagggcgagu ucaacuccaa gaacaacgac      540

auccagaaga acuuccagga cuuccuggac accuacaacg ccaucuucga gagcgaccug      600

ucccucgaga acagcaagca gcuggaggag aucgugaagg acaagaucuc caagcuggag      660

aagaaggacc ggauccucaa gcuguucccg ggggagaaga acagcggcau cuucuccgag      720

uuccugaagc ucaucgucgg gaaccaggcc gacuuccgca agugcuucaa ccuggacgag      780

aaggcgagcc ugcacuucuc caaggagagc uacgacgagg accucgagac ccugcugggc      840

uacaucggcg acgacuacuc cgacguguuc cucaaggcca agaagcugua cgacgccauc      900

cugcucagcg gguuccugac cgugacggac aacgagaccg aggcgccccu gagcuccgcc      960

augaucaagc gguacaacga gcacaaggag gaccucgccc ugcugaagga guacauccgc     1020

aacaucagcc ucaagaccua caacgagguc uucaaggacg acaccaagaa cggcuacgcc     1080

ggguacaucg acggcaagac gaaccaggag gacuucuacg uguaccugaa gaaccugcuc     1140

gccgaguucg agggcgccga cuacuuccug gagaagaucg accgggagga cuuccugcgc     1200

aagcagcgga ccuucgacaa cggguccauc cccuaccaga uccaccucca ggagaugcgc     1260

gccauccugg acaagcaggc gaaguucuac cccuuccugg ccaagaacaa ggagcgcauc     1320

gagaagaucc ugaccuuccg gaucccguac uacgugggcc cccucgcccg cgggaacagc     1380

gacuucgccu gguccauccg gaagcgcaac gagaagauca cccccuggaa cuucgaggac     1440

gucaucgaca aggagagcuc cgccgaggcg uucaucaacc ggaugacgag cuucgaccug     1500

uaccugcccg aggagaaggu gcucccgaag cacucccugc uguacgagac cuucaacgug     1560

uacaacgagc ucaccaaggu ccgcuucauc gccgagagca ugcgggacua ccaguuccug     1620

gacuccaagc agaagaagga caucgugcgc cuguacuuca aggacaagcg gaaggugacc     1680

gacaaggaca ucaucgagua ccuccacgcc aucuacggcu acgacgggau cgagcugaag     1740

ggcaucgaga agcaguucaa cagcucccug agcacguacc acgaccuccu gaacaucauc     1800

aacgacaagg aguuccugga cgacuccagc aacgaggcca ucaucgagga gaucauccac     1860

acccucacca ucuucgagga ccgcgagaug aucaagcagc ggcuguccaa guucgagaac     1920

aucuucgaca agagcguccu gaagaagcuc ucccgccggc acuacaccgg cugggggaag     1980

cugagcgcca agcugaucaa cggcauccgc gacgagaagu ccgggaacac gauccucgac     2040

uaccugaucg acgacggcau cagcaaccgg aacuucaugc agcugaucca cgacgacgcg     2100

cucagcuuca agaagaagau ccagaaggcc cagaucaucg gggacgagga caagggcaac     2160

aucaaggagg uggugaaguc ccugcccggc agccccgcca ucaagaaggg gauccugcag     2220

uccaucaaga ucgucgacga gcucgugaag gucaugggcg ggcgcaagcc cgagagcauc     2280

gugguggaga uggcccggga gaaccaguac accaaccagg gcaaguccaa cagccagcag     2340

cgccugaagc ggcuggagaa gucccucaag gagcugggga gcaagauccu gaaggagaac     2400

aucccggcga agcucuccaa gaucgacaac aacgcccugc agaacgaccg ccuguaccuc     2460

uacuaccugc agaacggcaa ggacauguac accggcgacg accuggacau cgaccgccuc     2520

agcaacuacg acaucgacca caucaucccc caggccuucc ugaaggacaa cuccaucgac     2580

aacaaggucc uggugagcuc cgccagcaac cgggggaagu ccgacgacuu ccccagccuc     2640

gaggugguca agaagcgcaa gaccuucugg uaccagcugc ucaagagcaa gcugaucucc     2700

cagcggaagu ucgacaaccu gacgaaggcc gagcgcggcg ggcugcuccc cgaggacaag     2760

gcgggcuuca uccagcggca gcugguggag acccgccaga ucaccaagca cguggcccgg     2820

cugcucgacg agaaguucaa caacaagaag gacgagaaca accgcgccgu ccggaccgug     2880

aagaucauca cgcucaagag cacccuggug ucccaguucc gcaaggacuu cgagcuguac     2940

aagguccggg agaucaacga cuuccaccac gcccacgacg ccuaccugaa cgcggugauc     3000

gccagcgccc ugcucaagaa guacccgaag cuggagcccg aguucgugua cggcgacuac     3060

cccaaguaca acuccuuccg cgagcgcaag agcgccaccg agaaggucua cuucuacucc     3120

aacaucauga acaucuucaa gaaguccauc agccuggccg acgggcgggu gaucgagcgg     3180

ccccucaucg aggugaacga ggagaccggc gagagcgucu ggaacaagga guccgaccug     3240

gcgaccgugc gccgggugcu gagcuacccg caggucaacg uggugaagaa ggucgaggag     3300

cagaaccacg ggcucgaccg cggcaagccc aaggggcugu ucaacgccaa ccuguccagc     3360

aagcccaagc ccaacuccaa cgagaaccuc gugggcgcca aggaguaccu ggacccgaag     3420

aaguacggcg gguacgccgg caucagcaac uccuucgcgg ugcuggucaa ggggacgauc     3480

gagaagggcg ccaagaagaa gaucaccaac gugcucgagu uccaggggau cagcauccug     3540

gaccggauca acuaccgcaa ggacaagcug aacuuccucc uggagaaggg cuacaaggac     3600

aucgagcuga ucaucgagcu ccccaaguac ucccuguucg agcugagcga cggcagccgg     3660

cgcaugcucg ccuccauccu gagcaccaac aacaagcgcg gggagaucca caagggcaac     3720

cagaucuucc ugucccagaa guucgugaag cuccuguacc acgccaagcg gaucagcaac     3780

accaucaacg agaaccaccg caaguacguc gagaaccaca agaaggaguu cgaggagcug     3840

uucuacuaca uccucgaguu caacgagaac uacguggggg ccaagaagaa cggcaagcug     3900

cugaacuccg cguuccagag cuggcagaac cacuccaucg acgagcucug cagcuccuuc     3960

aucgggccca cgggcagcga gcggaagggc cuguucgagc ugaccucccg cgggagcgcc     4020

gccgacuucg aguuccucgg cgugaagauc ccgcgguacc gcgacuacac ccccuccagc     4080

cugcugaagg acgccacccu cauccaccag uccgucacgg ggcuguacga gacccggauc     4140

gaccuggcca agcugggcga gggguga                                         4167


<210>  2072
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2072
augaccaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucacggaca acuacaaggu gccgagcaag aagaugaagg ugcucgggaa caccuccaag      120

aaguacauca agaagaaccu gcugggcguc cuccuguucg acagcgggau caccgccgag      180

ggccgccggc ugaagcgcac cgcgcggcgc cgguacacgc gccggcgcaa ccggauccuc      240

uaccugcagg agaucuucuc caccgagaug gccacccugg acgacgccuu cuuccagcgc      300

cucgacgaca gcuuccuggu gcccgacgac aagcgggacu ccaaguaccc caucuucggc      360

aaccuggugg aggagaaggc cuaccacgac gaguucccca ccaucuacca ccuccgcaag      420

uaccuggccg acagcacgaa gaaggcggac cugcggcucg ucuaccuggc ccuggcccac      480

augaucaagu accgcgggca cuuccucauc gagggcgagu ucaacuccaa gaacaacgac      540

auccagaaga acuuccagga cuuccuggac accuacaacg ccaucuucga gagcgaccug      600

ucccucgaga acagcaagca gcuggaggag aucgugaagg acaagaucuc caagcuggag      660

aagaaggacc ggauccucaa gcuguucccg ggggagaaga acagcggcau cuucuccgag      720

uuccugaagc ucaucguggg gaaccaggcg gacuuccgca agugcuucaa ccuggacgag      780

aaggccagcc ugcacuucuc caaggagagc uacgacgagg accucgagac ccugcugggc      840

uacaucggcg acgacuacuc cgacgucuuc aucaaggcca agaagcucua cgacgccauc      900

cugcugagcg gguuccucac cgugacggac aacgagaccg aggccccgcu gagcuccgcg      960

augaucaagc gguacaacga gcacaaggag gaccuggccc uccugaagga guacauccgc     1020

aacaucagcc ugaagaccua caacgaggug uucaaggacg acaccaagaa cggcuacgcc     1080

ggguacaucg acggcaagac gaaccaggag gacuucuacg ucuaccucaa gaaccugcug     1140

gccgaguucg agggcgccga cuacuuccuc gagaagaucg accgggagga cuuccugcgc     1200

aagcagcgga ccuucgacaa cggguccauc cccuaccaga uccaccucca ggagaugcgc     1260

gcgauccucg acaagcaggc caaguucuac cccuuccugg ccaagaacaa ggagcgcauc     1320

gagaagaucc ugaccuuccg gaucccguac uacgugggcc cccuggcccg cgggaacagc     1380

gacuucgccu gguccauccg gaagcgcaac gagaagauca cccccuggaa cuucgaggac     1440

gugaucgaca aggagagcuc cgcggaggcc uucaucaacc ggaugacgag cuucgaccuc     1500

uaccugcccg aggagaaggu ccugccgaag cacucccucc uguacgagac cuucaacgug     1560

uacaacgagc ugaccaaggu gcgcuucauc gccgagagca ugcgggacua ccaguuccuc     1620

gacuccaagc agaagaagga caucguccgc cuguacuuca aggacaagcg gaaggugacc     1680

gacaaggaca ucaucgagua ccugcacgcc aucuacggcu acgacgggau cgagcucaag     1740

ggcaucgaga agcaguucaa cagcucccug agcacguacc acgaccugcu caacaucauc     1800

aacgacaagg aguuccugga cgacuccagc aacgaggcca ucaucgagga gaucauccac     1860

acccugacca ucuucgagga ccgcgagaug aucaagcagc ggcucuccaa guucgagaac     1920

aucuucgaca agagcgugcu gaagaagcug ucccgccggc acuacaccgg cugggggaag     1980

cucagcgcga agcugaucaa cggcauccgc gacgagaagu ccgggaacac gauccuggac     2040

uaccucaucg acgacggcau cagcaaccgg aacuucaugc agcugaucca cgacgacgcc     2100

cugagcuuca agaagaagau ccagaaggcc cagaucaucg gggacgagga caagggcaac     2160

aucaaggagg ucgugaaguc ccuccccggc agccccgcca ucaagaaggg gauccugcag     2220

uccaucaaga ucguggacga gcuggucaag gugaugggcg ggcgcaagcc cgagagcauc     2280

gucguggaga uggcgcggga gaaccaguac accaaccagg gcaaguccaa cagccagcag     2340

cgccucaagc ggcuggagaa gucccugaag gagcucggga gcaagauccu gaaggagaac     2400

aucccggcca agcuguccaa gaucgacaac aacgcccucc agaacgaccg ccuguaccug     2460

uacuaccucc agaacggcaa ggacauguac accggcgacg accuggacau cgaccgccug     2520

agcaacuacg acaucgacca caucaucccc caggccuucc ucaaggacaa cuccaucgac     2580

aacaaggugc uggucagcuc cgccagcaac cgggggaagu ccgacgacuu ccccagccug     2640

gaggugguga agaagcgcaa gaccuucugg uaccagcucc ucaagagcaa gcugaucucc     2700

cagcggaagu ucgacaaccu gacgaaggcg gagcgcggcg ggcugcugcc cgaggacaag     2760

gccggcuuca uccagcggca gcucgucgag acccgccaga ucaccaagca cguggcccgg     2820

cugcuggacg agaaguucaa caacaagaag gacgagaaca accgcgccgu gcggaccguc     2880

aagaucauca cgcucaagag cacccuggug ucccaguucc gcaaggacuu cgagcuguac     2940

aaggugcggg agaucaacga cuuccaccac gcccacgacg cguaccucaa cgccgucauc     3000

gccagcgccc ugcugaagaa guacccgaag cucgagcccg aguucgugua cggcgacuac     3060

cccaaguaca acuccuuccg cgagcgcaag agcgccaccg agaaggugua cuucuacucc     3120

aacaucauga acaucuucaa gaaguccauc agccuggcgg acgggcgggu caucgagcgg     3180

ccccugaucg aggugaacga ggagaccggc gagagcgugu ggaacaagga guccgaccuc     3240

gccaccgucc gccgggugcu gagcuacccc caggugaacg ucgugaagaa gguggaggag     3300

cagaaccacg ggcuggaccg cggcaagccc aaggggcugu ucaacgccaa ccuguccagc     3360

aagcccaagc ccaacuccaa cgagaaccug gucggcgcca aggaguaccu cgacccgaag     3420

aaguacggcg gguacgcggg caucagcaac uccuucgccg ugcuggugaa ggggacgauc     3480

gagaagggcg ccaagaagaa gaucaccaac guccuggagu uccaggggau cagcauccuc     3540

gaccggauca acuaccgcaa ggacaagcug aacuuccugc ucgagaaggg cuacaaggac     3600

aucgagcuga ucaucgagcu gcccaaguac ucccucuucg agcugagcga cggcagccgg     3660

cgcaugcugg ccuccauccu cagcaccaac aacaagcgcg gggagaucca caagggcaac     3720

cagaucuucc ugucccagaa guucgugaag cugcucuacc acgccaagcg gaucagcaac     3780

accaucaacg agaaccaccg caaguacgug gagaaccaca agaaggaguu cgaggagcug     3840

uucuacuaca uccuggaguu caacgagaac uacgucgggg cgaagaagaa cggcaagcuc     3900

cugaacuccg ccuuccagag cuggcagaac cacuccaucg acgagcugug cagcuccuuc     3960

aucgggccca cgggcagcga gcggaagggc cucuucgagc ugaccucccg cgggagcgcc     4020

gccgacuucg aguuccuggg cgugaagauc cagcgguacc gcgacuacac cccguccagc     4080

cuccugaagg acgccacccu gauccaccag uccgugacgg ggcucuacga gacccggauc     4140

gaccuggcga agcugggcga gggguga                                         4167


<210>  2073
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2073
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga acgagaagga gaucuccaag aucaucaagc ucgggguccg ggugaacccc      120

cugaccaucg acgagaagac gaacuucgag aagggccgcc cgcugagcac caacgcggac      180

cggacccuca agcgcggggc ccggcgcaac cugcagcggu acaagcugcg ccgggagaac      240

cucaucgaga uccugaucga caaccacauc aucaccaagg agacgccccu gaccgagauc      300

ggcaagggca ccaccuacca gacgcucucc cugcgcgcca aggccgugcg ggagcgcauc      360

gaccuggagg acuucgccaa gguccuccug gcgaucaaca agaagcgggg guacaagagc      420

ucccgcaagg ccagcaacga cgaggagggc caggccaucg acgggauggc caucgcgaag      480

gagcuguacg agcggggcau cacccccggg caguacgugc ucgagcugcu ggagaacgac      540

cgcaaguaca uccccgacuu cuaccggucc gaccucaagc aggaguucaa caagaucugg      600

aacaaccaga agaccuucua cccggagauc cugaccgacg agcuguucaa ggagcucgac      660

ggcaagaaca agagccagac gugggccauc ugccagaagc ccuucaacau caagggcauc      720

aagcgcauca ccaaggggcg ggagaagacc aucgagaacu accacuggcg cgugaagggc      780

cuguccggga agcugaaccu cgagaagcug gccaucaucc ugcaggagau caacaacgac      840

aucaaccaga gcuccggcua ccucggcggg aucagcgaca aguccaagaa gcuguaccug      900

aacaaggaga ccgucggcga guuccucuac aagcagaucg ccaagaaccc ccacacgagc      960

cugaagaacc agguguucua ccggcacgac uaccuggacg aguucgagca gaucuggaag     1020

acccagucca acuucaacgc caaccucacc gacgagcuga agcaggagau ccgcgacgug     1080

aucaucuucu accagcgcaa gaucaagagc cagaaguccc ugaucagcuu cugccaguuc     1140

gaguccuggg agcaggagua cuucgacaag gagaccggga agacgaagac ccggaccguc     1200

ggccgccggg ugauccccaa gagcagcccc cucuuccagg aguucaagau cuggcagaac     1260

cugaaccacc ugguguucac caacuccgag acggacgaga agaucgagau caaggagguc     1320

gagcaggaga uccgcaagga gcuguucgag gagcugaacc ugcgcgggga ccucaacccc     1380

aacgaccugc ugaagauccu ccggagccag uucaagaucg gcaagcuguc caacuggaag     1440

ugcaacuucg agaagaucga gggcaaccgg accaacgcgg cccuguucaa caucuaccag     1500

aagaucagcg acuucgaggg guacggcauc gacuggaaca aguccaacgc caagaagauc     1560

cgcaaggagc ugaaggccac cuuccccgag aucgggaucg acgccagcau ccucgaguuc     1620

gacgcguaca agaagcagcu gaccaaccag uccagcuacg agcuguggca ccuccuguac     1680

uccgccgagg aggacuucaa gaucagcgag caggacaagc ugaucuacgg caacuccagc     1740

gugucccuca agaagaagcu gcaccugaag uucggguuca accccgagua cgccaagaug     1800

cucgccaacc ugagccugau guccgacuac ggcaaccuca gcgccaaggc gaucaagaag     1860

auccugccgu accuggagga cggccaccuc uacuccgagg ccugccggcu ggccggguac     1920

aaccacagca agucccugac ggccgaggag cucgagaagu ucgagcccaa ggccaagcug     1980

gaccugcuca agaagaacag ccugcgcaac cccguggucg agaagauccu gaaccagaug     2040

gugaacgugg ucaaccaggu gaucgacacc uacggcaagc ccgacggggu gcggaucgag     2100

cucgcgcgcg agcugaagaa guccgccaag gagcgggccg agaugaccaa gggcaucgcc     2160

gacgggaccc gccggaacga ggagaucaag aagaucauca cgcagaaguu cggcaucccg     2220

aaccccacca aguccgaccu gguccgcuac cggcucuggc aggagcugga gagccgcggc     2280

uacaaggaca ucuucaccga caaguacauc ucccccgaca agaucuucag cuccgagauc     2340

gacaucgagc acaucauccc caaggcgcuc cucuucgacg acagcuucuc caacaagacc     2400

cugagcuucc ggaacgugaa ccugaagaag gccaaccgca cggcccucga cuucaucacc     2460

caggacuacc aguccaacgu ggaggccuac aaggagcgcg ucgagaccug gaacaaggac     2520

aaccacauca gcaaggggaa gcggaacaag cugcugaugu cccucaagga caucccggac     2580

ggcuucaucg agcgcgaccu gcggaacagc caguacaucg cccgcaaggc gaagcggaug     2640

cuguacgagg uguuccccaa cgagguccuc gccaccuccg ggagcaucac cgacaagcug     2700

cgcuccgacu gggaccugau caacgugaug aaggagcuca accugcccaa guacaaggcc     2760

cugggccuca cggaguacga ggagcggcug gacaagggca ccgaggagcu guucaagcac     2820

gaggugauca aggacuggac caagcgcaac gaccaccggc accacgccau ggacgcccuc     2880

gcggucgccu ucaccacgca caaccacauc caguacauca acaaccugaa cgcccgcaag     2940

gacaucaccc aggacaacca ccccaucauc aagaacaucg agaaccugau caccgaggug     3000

aacagcaagg ggaagcgggu guucaaggcc ccgaugucca gcuuccgcga gagcgccaag     3060

gagcagaucg aggcgauccu cgucuccuuc aagaacaaga acaagguggu gaccaagaac     3120

aucaaccaga cgaagaagga gggcggguuc aacuaccaga acgccaagac cccgcggggc     3180

cagcugcaca aggagaccau cuacggcaag agcaagcagc cccuggagaa gcccgucaag     3240

aucaacaacc gcuucugcaa gaagcagguc gggcucauca ucaacuucaa ggagcaggag     3300

gccgugcugc accaccugaa gaaguacgac aacaacgaga agaucgccuu cuccaacaag     3360

acccucaaga gcgacccgau ccaggugaac ggcagccccc ugcgggaggu gaagaucuuc     3420

gaggagaucu ucacgauccg caaggacauc aaccccgaca acuucaagac ccagaagcac     3480

cuggacaaga ucaucgacaa ggggaucaag gaggugauga agaagcggcu cgaggaguac     3540

aacggcgacu ucaagaccgc guucagcgac cuggacaaga acccgaucug gcugaaccag     3600

uccaagggga ucaccaucaa gcgcgccacg aucagcggcg ucaccaacgc cgagccccuc     3660

caccaggcca aggaccaccu ggggaacauc auccuggacg acaacggcaa ggagaucccc     3720

aucgacuacg uguccaccgg caacaaccac cacguggcca ucuaccagga cgagagcggg     3780

aaccuccagg aggaggucgu guccuucuac gaggcgguga uccggaagaa ccaggaccag     3840

cccaucauca agaagcgcca cccgaagggc uggaaguucc uguucaccau gaagcagaac     3900

gagauguucg ucuucagcuc cgagaacuuc aaccccagcg agaucgaccu gcucgacgag     3960

aagaacgccg ccaucaucuc ccccaaccug uuccgggugc agaagaucgc cacgaagaac     4020

uacauguucc gccaccaccu ggagaccgag gugaccaaca accucgacuu caccuaccgc     4080

cacuuccagg ccacggagug gcugcggaac cugugcaagg uccgcaucga ccacaucggg     4140

cggaucgugc agaucggcga guacuga                                         4167


<210>  2074
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2074
augaucaucc gcaccuacga gcugaagacc aucacgacca ucgccauccu caagauguuc       60

cagcugcuga ccggcgggcu cagcaugacc aagcugggca agcccuacgg gaucggcgug      120

gacaucgggu ccaacagcau cggcuucgcc gcggucgacg agaacuccca ccugauccgg      180

cucaagggca agacggugau cggggcccgc cuguucgagg agggcaaggc cgccgccgac      240

cggcgcgcga gccggaccac ccgccggcgc cugucccgga accgcuggcg gcucagcuuc      300

cugcgcgacu ucuucgaguc ccacaucacc ccgacggggc ccaacuucuu caugcggcag      360

aaguacagcg agaucucccc caaggacaag gcccgcuaca aguacgagaa gcggcuguuc      420

aacgaccgca ccgacgccga guucuaccag caguacccca ccauguacca ccuccggaac      480

cgccugcuga ccgacccgag caaggccgac gugcgggaga ucuacuucgc gauccaccac      540

auccucaagu cccgcggcca cuuccugacg agcggguacg ccaaggacuu caacaccaac      600

aaggucgccc ugaacgagau cuuccccgcc cuccaggacg ccuacgccca gguguacccc      660

gaccuggaca ucaccuucga cgagaacaag gugaacgagu ucaagaccgu ccugcucaac      720

gagaaggcga cgcccuccga cacccagcgg gcccugguga accugcuccu ggccgaggac      780

ggcgacaagg acauccugaa gcagcagaag cagguccuca ccgaguucgc caaggcggug      840

gugggccuga agaccaagcu gaacguggcc cucgggacgg aggucgacag cuccgaggcc      900

accgugugga acuucagccu gggccagcug gacgacaagu gggccgggau cgaguccgcc      960

augaccgacg agggcaccga gauccucgac cagauccgcg accuguaccg cgcgcggcug     1020

cucaacggca ucgugccggc cgggaagacg cugagccagg ccaaggucga cgacuacgcc     1080

cagcaccacg aggaccugga gcuguucaag gacuaccuga agcagcugga ggacgacggc     1140

accgccaagg cgauccgcca gcucuacgac cgguacaucg acggggacga cgccaagccc     1200

uucgugcgcg aggacuucgu gaaggcccug accaaggagg ucaccgccca ccccaacgcg     1260

aagucccccg agcugcucga gcggcuggcc cagccggacu ucaugcugaa gcagcgcaac     1320

aaggccaacg gcgccauccc cgugcagaug cagcagcggg agcucgacca gaucaucaag     1380

aaccagagcg uguacuacga cuggcuggcc gcgcccaacc ccgucgagaa gcaccgcaag     1440

agcaugccgu accagcugga cgagcugcuc aacuuccgga uccccuacua cguggggccc     1500

cuggugacgg ccaaggagca gaaggccgcc cagggcggcg ucuucgccug gauggugcgc     1560

aaggaccccg acgggaacau caccccguac aacuucgacg agaaggucga ccgggaggcg     1620

uccgccaaca ccuucaucca gcgcaugaag accacggaca ccuaccugau cggcgaggac     1680

gugcucccca agcagagccu gcuguaccag cgguacgagg ugcucaacga gcugaacaac     1740

guccgcguga acaacgagaa gcuguccauc gagcagaagc agcaggucau ccgggagcug     1800

uucgagcgcc acaacagcgu gaccaucaag caguucgccg agaaccuccg ggcccacggg     1860

gacuacgccc acauccccga gauccgcggc cuggcggacg agaagcgguu ccucuccagc     1920

cuguccaccu accgccagcu caagagccuc cugcccgacg ccaucgacga cccggccaag     1980

caggccgaca ucgagaacau caucgcgugg uccacggugu ucgaggacgc cgccaucuuc     2040

aagaccaagc ugaaggagau caacuggcuc gacagccaga ccaucaccaa gcuguccaac     2100

auccgcuacc gggggugggg ccaguucagc cacaaguucc ugaacggccu cacccugggg     2160

aacggccaca cgaucaucca ggagcuguuc cucuccaaca acaaccugau gcagauccug     2220

accgacgaga cccuccagaa gaagaugacc gagcugaacg ccgacaagcu gaaggccgcg     2280

aacaucaacg acgccaucga cgacgccuac acgagccccu ccaacaagaa ggcccuccgc     2340

caggugcugc gggucaucga ggacaucaag cgcgccgcgg acgggcagga ccccagcugg     2400

cuguacgugg agaccgccga cggcgggggc acccccggca agcggacccg cgcccggcag     2460

caccagcucc aggagaucua cgccaacgcc gcgcacgagc ugaucgacuc cgccgugcgc     2520

ggggagcugg aggacaagau cgccgacaag gccgacuuca acgaccggcu cguccuguac     2580

uucaugcagg gcgggcgcga caucuacacg ggcaccccgc ugaacaucga ccagcucagc     2640

uccuacgaca ucgaccacau ccugccccag agccugauca aggacaacag ccucgacaac     2700

cgggugcugg ugcacgcgau caucaaccgc gagaagaacg ccaccuucgc cuccaccauc     2760

uacgcccaga agaugaacgc cacguggcgg caguggcacg aggcgggccu gaucagcggg     2820

cgcaagcucc ggaaccugca gaugcgcccc gaccagaucg acaaguacgc cuccggcuuc     2880

gucgcccggc agcuggugga gacccgccag aucaucaagc ucaccgagca gaucguggcc     2940

gcccaguacc cggagaccaa gaucaucgcg gucaaggccg ggcugagcca ccagcugcgg     3000

gaggagcucg aguuccccaa gaaccgcgac gugaaccacu accaccacgc cuucgacgcc     3060

uuccuggccg cgcggaucgg cacguaccug cucaagcgcu uccccaaccu gcaggccuuc     3120

uucaccuacg ggaaguucaa gaaggccgac gugaagaagc ugcgcggcuu caacuucauc     3180

cgggacauca cccacgccga ggacaagauc gucgugaagg acaccggcga ggugaucugg     3240

gacaagaagc gcgacgucga cgagcucgac cggaucuaca acuucaagcg caugcugauc     3300

acgcacgagg ugcacuucga gaaggccgac cuguucaagc agaccgugua cggggcgaag     3360

gacuccaagg aggccggcgg gaagaagcag cucaucagca agaagaaggg cuaccccguc     3420

gacaucuacg ggggcuacac ccaggagacc ggcuccuacc ugagcguggu gcggcugacg     3480

aagaaggcca uguacgccgu cgugaaggug uccacccgcg acgcggccaa gcucgccguc     3540

gccaagagca ucuccgagca gaaggagaac gagacccuga acgacaucau cgacgagaag     3600

cugagcaaga ucuccaagaa cgggaagauc acccacgagc uguucgaggu ggugcucccg     3660

cgggucggcc agaagacgcu guucaagaac agcaaguaca accucuuccu ggugaacucc     3720

gacaccuaca ugcacaacua ccaggagcug uggaugcccc gcgaguacca gaagaugugg     3780

aaggacauca gccucuccaa guacggggac gcccagaccg aggaccagcu ggaccagauc     3840

uucggcuuca ucgugagcca ggucaacagc uacuucaacc uguacgacau caaccaguuc     3900

cggaagaagc ucaacgacgc ggccgacaag uucgccaccc ugcccaugcg cgacacggac     3960

gacaugcagg ggaagaucgc caccaucggc cagcugcuca ucggccugca ggccaacgcg     4020

gcccgguccg accugcgcaa ccucgacauc aagacgcccc uggggcugcu ccagaucccg     4080

aacggcauca gccuggacuc cgacaccagc gugguguacc agucccccac cgggcugcuc     4140

gagcgggagg uccaccugag cgaccuguga                                      4170


<210>  2075
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2075
auggcccgcg acuacagcgu gggccuggac aucgggaccu ccagcgucgg cugggccgcg       60

aucgacaaca aguaccaccu cauccgggcc aaguccaaga accugaucgg ggugcgccug      120

uucgacagcg ccgugaccgc cgagaagcgg cgcggcuacc ggacgacccg ccggcgccuc      180

ucccggcgcc acuggcggcu gcgccugcuc aacgacaucu ucgccgggcc ccugaccgac      240

uucggcgacg agaacuuccu ggcgcggcuc aaguacagcu ggguccaccc gcaggaccag      300

uccaaccagg cccacuucgc cgccggccug cuguucgaca gcaaggagca ggacaaggac      360

uucuaccgca aguaccccac caucuaccac cuccggcugg cgcugaugaa cgacgaccag      420

aagcacgacc uccgcgaggu guaccuggcc auccaccacc uggugaagua ccgggggcac      480

uuccucaucg agggcgacgu caaggccgac uccgccuucg acgugcacac guucgccgac      540

gcgauccagc gcuacgccga gagcaacaac uccgacgaga accugcuggg gaagaucgac      600

gagaagaagc ucagcgccgc ccugaccgac aagcacggcu ccaagagcca gcgggccgag      660

accgcggaga ccgccuucga cauccuggac cuccagucca agaagcagau ccaggccauc      720

cucaagagcg uggucgggaa ccaggccaac cugauggcca ucuucggccu ggacuccagc      780

gcgaucucca aggacgagca gaagaacuac aaguucagcu ucgacgacgc cgacaucgac      840

gagaagaucg ccgacagcga ggcccugcug uccgacacgg aguucgaguu ccucugcgac      900

cugaaggccg cguucgacgg ccugacccuc aagaugcugc ugggggacga caagaccgug      960

agcgccgcca uggugcgccg guucaacgag caccagaagg acugggagua caucaagucc     1020

cacauccgca acgccaagaa cgcgggcaac gggcucuacg agaaguccaa gaaguucgac     1080

ggcaucaacg ccgccuaccu ggcccugcag agcgacaacg aggacgaccg caagaaggcc     1140

aagaagaucu uccaggacga gaucagcucc gcggacaucc ccgacgacgu caaggccgac     1200

uuccucaaga agaucgacga cgaccaguuc cugcccaucc agcggaccaa gaacaacggc     1260

acgaucccgc accagcugca ccgcaacgag cucgagcaga ucaucgagaa gcaggggauc     1320

uacuaccccu uccugaagga caccuaccag gagaacagcc acgagcugaa caagaucacc     1380

gcccucauca acuuccgggu gcccuacuac gugggccccc uggucgagga ggagcagaag     1440

aucgccgacg acgggaagaa caucccggac cccaccaacc acuggauggu gcgcaagucc     1500

aacgacacga ucacccccug gaaccugagc cagguggucg accucgacaa guccggccgg     1560

cgcuucaucg agcggcugac cgggaccgac acguaccuga ucggcgagcc cacccugccg     1620

aagaacagcc uccuguacca gaaguucgac gugcugcagg agcucaacaa cauccgcgug     1680

uccggccggc gccuggacau ccgggccaag caggacgcgu ucgagcaccu guucaagguc     1740

cagaagaccg ugagcgccac caaccucaag gacuuccugg ugcaggccgg guacaucucc     1800

gaggacacgc agaucgaggg ccuggccgac gucaacggga agaacuucaa caacgcccuc     1860

accaccuaca acuaccuggu gagcguccug ggccgcgagu ucguggagaa ccccuccaac     1920

gaggagcucc uggaggagau caccgagcug cagacggugu ucgaggacaa gaagguccuc     1980

cggcgccagc uggaccagcu ggacgggcuc agcgaccaca accgggagaa gcugucccgc     2040

aagcacuaca ccggcugggg ccggaucagc aagaagcugc ucaccaccaa gaucgugcag     2100

aacgcggaca agaucgacaa ccagacguuc gacgugcccc gcaugaacca gagcaucauc     2160

gacacccugu acaacaccaa gaugaaccug auggagauca ucaacaacgc cgaggacgac     2220

uucggggucc gggccuggau cgacaagcag aacaccacgg acggcgacga gcaggacgug     2280

uacucccuca ucgacgagcu ggccgggccc aaggagauca agcgcggcau cgugcagagc     2340

uuccgcaucc uggacgacau caccaaggcg gucggguacg ccccgaagcg gguguaccuc     2400

gaguucgccc gcaagaccca ggagucccac cugaccaaca gccggaagaa ccagcugucc     2460

acccuccuga agaacgccgg ccugagcgag cucgugacgc aggucuccca guacgacgcc     2520

gcggcccugc agaacgaccg ccuguaccuc uacuuccugc agcagggcaa ggacauguac     2580

agcggggaga agcugaaccu cgacaaccug uccaacuacg acaucgacca caucaucccc     2640

caggccuaca ccaaggacaa cagccuggac aaccgggugc ucguguccaa caucaccaac     2700

cgccgcaaga gcgacuccag caacuaccug cccgcccuga ucgacaagau gcggcccuuc     2760

ugguccgucc ucagcaagca gggccugcug uccaagcaca aguucgccaa ccucacccgg     2820

acgcgcgacu ucgacgacau ggagaaggag cgguucaucg cgcgcagccu gguggagacc     2880

cggcagauca ucaagaacgu ggccagccug aucgacuccc acuucggggg cgagaccaag     2940

gccgucgcca uccgcagcuc ccucaccgcc gacaugcggc gcuacgugga caucccgaag     3000

aaccgggaca ucaacgacua ccaccacgcg uucgacgccc ugcuguucag cacggugggc     3060

caguacaccg agaacuccgg gcucaugaag aagggccagc ugagcgacuc cgccgggaac     3120

caguacaacc gcuacaucaa ggaguggauc cacgccgcgc ggcugaacgc ccagagccag     3180

cgcgucaacc ccuucggcuu cguggugggg uccaugcgga acgccgcccc cggcaagcuc     3240

aaccccgaga ccggcgagau caccccggag gagaacgccg acuggagcau cgcggaccug     3300

gacuaccugc acaaggucau gaacuuccgc aagaucacgg ugacccgccg gcucaaggac     3360

cagaaggggc agcuguacga cgagucccgc uaccccagcg ugcugcacga cgccaagucc     3420

aaggccagca ucaacuucga caagcacaag cccgucgacc ucuacggcgg guucuccagc     3480

gccaagcccg ccuacgcggc ccugaucaag uucaagaaca aguuccggcu ggugaacgug     3540

cuccgccagu ggaccuacuc cgacaagaac agcgaggacu acauccugga gcagauccgg     3600

ggcaaguacc cgaaggccga gaugguccug ucccacaucc ccuacgggca gcucgugaag     3660

aaggacggcg cccuggugac caucagcagc gccacggagc ugcacaacuu cgagcagcuc     3720

uggcugcccc uggcggacua caagcucauc aacacccugc ugaagaccaa ggaggacaac     3780

cucgucgaca uccugcacaa ccgccuggac cucccggaga ugaccaucga guccgccuuc     3840

uacaaggccu ucgacagcau ccuguccuuc gccuucaacc gguacgcccu gcaccagaac     3900

gcgcucguga agcugcaggc ccaccgcgac gacuucaacg cccugaacua cgaggacaag     3960

cagcagacgc ucgagcggau ccuggacgcc cugcacgcga gccccgccuc cagcgaccuc     4020

aagaagauca accuguccag cggcuucggg cgccuguucu cccccagcca cuucacccuc     4080

gccgacaccg acgaguucau cuuccagucc gugaccggcc uguucagcac gcagaagacc     4140

gucgcccagc uguaccagga gaccaaguga                                      4170


<210>  2076
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2076
augaagaacu acaacaucag ccuggugccc uccccgagcg gcgucgggua cgccgugacc       60

gacgacaacc ucaacaucau caagcccgug caccacgucu ccggcaucgg gagccgccug      120

uucaacgagg gcuccagcaa gcaggaccgg cgcaacuccc ggagcgcccg ccggucccgc      180

caccggcagc aggggcgcau caaggcgcug aacagcauca ugaaggacga gaucaucaag      240

guggacccca ccuucuucga ccggcucuac caguccgagc ugagcccccu ggacaagaac      300

aagaaguacc gcaacaucau cuucgacaag ccguccgugg agagcuacua cuacaagaag      360

uuccccacga ucuaccaccu cgagaaguac cugaucaaca ccgacggcaa ggccgacauc      420

cggcugaucu acugggcccu ccacucccug cugacccacc gcggccacuu cuacaacacc      480

acgcccguca gccaguucaa gcccggggcc cucaacauca aggacaaguu caucgagcug      540

aaccagcuga accaggugaa gaccuucgag uucuccgugg ccaacacccc ggagaucgag      600

cacauccuca aggaccggua caucagcaag cagaacaaga ucaaccagcu ggcggagcuc      660

cucuucgagc aggagcccga gaaggacauc cagaaggcca acaccaaggu cgccaagacg      720

aucgccaagg cgauccuggg caacaaguuc cagcuggacg agaucaucgg gcucgacgug      780

aacaaguccg agaagaagga guggagccug cagcugagcg accucaaccu ggacuccaag      840

aucgacgagc ugagcgacuc ccucaccgac cagcagcugc gcauccugga gauccuccag      900

gagauguacg ccgccaccac ccugcuggac auccuccagg gcagcgacuc ccugcuggac      960

gccaagauca agagcuacaa cgaguacaag cagaaccggc uccuguucuu cgaccugcuc     1020

aagacgcugc ccgacaagaa ggcccaccuc cugcgccagg gguacacccu guaccugaac     1080

aaccaccacc gggaccucgc guccgcccgc aaggaccugg gcaucguggc cgccaccaac     1140

uucagcaaga ccgaccugua cgcccucauc aagaagacgc ugaaggacga gcccgagacc     1200

ccggagucca agaagguccu gggccugauc gacaaggggg aguuccucaa caagcagcgg     1260

accgaccuga acaccuacau ccccuaccag cugaacgcgc ucauccugaa caagauccug     1320

aagaaccagg gcaagcacua ccccuuccuc aucaaggaga accccgccga gccggaccgc     1380

aaggaggccc ccuacgagau cagccagcug augcaguuca cgauccccua cuacugcggg     1440

ccccuggcca ccccggagga gcagaagaac cuccccgagu ccagccgguu cggcuggcug     1500

gugcgcaagg aggagggccg caucaccccc uggaacuucu acaacaagau caacgugauc     1560

gagaccgcca acgcguucau caagcggucc aucgggaagg acacguaccu gcucagcgag     1620

cccguccugc cggacaacuc ccugcucuac cagaaguaca aggugcugaa cgagcugagc     1680

uccaucagcc ucguggacgc caacaccaag aaggagaaga agcugaccgc caagaucaag     1740

cagcugcucu acaaggaggg cuucaagaag cacgucaccg uguccaagac gauggcccug     1800

aagauccuga acgacaacaa cgugcccacc agcgacaucc ucgggcugag caccggcaag     1860

aaguucgaca acucccugag caccuacaac uccuggaaga aggccuuccc cgagcagauc     1920

gacaaccccc acuaccgcca ggaccucgag uacaugaucg aguggagcac ggucuucacc     1980

gaccggaaga uccuggugga gaagcugaag gagaucgagu ggcuccgccc ggagcagauc     2040

aaguucgugc uggagcaccg gcugaccggg uggggcaagc ucuccaagcg ccugcugguc     2100

gagcucaagg acaacaaggg caagagcaac aucgacaacc uguucaacac caagaagaac     2160

uucaugcaga ucguguccca gcaggucuac aacacgcaga ucgaggcgau cgcccugaag     2220

accaccaaga accagagccu cgacaacauc cuggaggccg ccuacaccuc ccccgcgaac     2280

cggaaggcca uccgccagac caugaaggug gugaacgaga ucgucacgcu ggccgggaag     2340

ggcacccacc ccgacaagau cuuccucacc uuccagcgga gcaaggagca ggaggggaag     2400

cugaccacgg accgcgccuc cagccugcuc aagaucuacc ggggcaucaa ggacccgcug     2460

ugcaccaagg agcuggugga cgagcucucc aagaacgugc gcgccaaccu gaucaagacc     2520

aaggaguacc uguacuacca gcagcucggg cgggacgcgc ugaccggcga gaucaucgac     2580

aagacggacc ugcgcaacua cgccguccuc cacaucaucc cccggagcgu gguggucgac     2640

gacuccauca acaaccuggu gcugaccaag gugaagaacg ccaaggagag caucgucgug     2700

caguacggcu ccaagaacau cagcgacauc agcauguccg ugaaggacuu cuggaaccgc     2760

cuccagaagc uggggcuguu cagcaagggc aagcuccgga accugaccac cgaccugacg     2820

gagcucaacc aguacgagaa gcagggguuc aucgcccgcc agcuggucga gaccaaccag     2880

aucgugaagc ugcucgccac cauccugcag ggccaguucc cccacaccaa gaucaucgag     2940

gugcggcagg agcagaucgc gaacauccgc uaccagcugg acaucuaccg ccuccgggac     3000

cugaacgucu acuaccgcgg gauggacgcc uaccuggccg ccgugguggg cacguaccuc     3060

uacaccgucu accccaaggc ccggcgcuuc uucguguacg gcaaguaccu gaagcaggac     3120

aagaacaacc gggaggaggg gcaguucaag accuccggcc acagcuucaa cuuccugugg     3180

aagcuguucu acgggggcga ggacgagauc uacgugcagg gcaccaacaa caucgcguuc     3240

aacaagaagg accugcugca gaagaucaag gaggucaaca acuacaaguu ccagaacgug     3300

ucccucgcca cggggacccg ccggagcgac cuguucaagc agaccaucua cccgcgccag     3360

gagcgggacg ugaagaaguc ccgcacccug aucacgaaga agaacggccu cuucaccgac     3420

aucuacgggg gcuacaccgg gaacgucaac agcuacuucg ugcuggugca gaccaaguac     3480

aagaaggacc ccgacaaguu caaccuguac ggcgucccca accgguacgu ggccaugcuc     3540

gagcagucca agagcaaggg caacuacgag acgaagcuga aggaggugau ggagcccauc     3600

aucaagaacu ccaacaagcg caugaucggg uacaagaucc ugaaggaccg gauccucuac     3660

aaccagauca ucgaggacaa cggcuacaag uucuucauca ccagcuccca guaccgccac     3720

aacuuccggc agaugauccu gagcgaccag uccaagaaga uccugaugga cuacgucuuc     3780

gacccgaacu ucuaccgcca caagaagacc gaggacguga acaacgacaa ggacgccaag     3840

cucaucaagg uguacgacga gauccuguac cagaucgacc acuaccugca gaucuucacc     3900

aucgggcacc acaucgacaa gcucaagaag gcgcggcccc uguucgagaa gugcacgcug     3960

gccgagaagg cccagguccu gaacgacauc cucaucaucg ccggcagcaa ccagaccgag     4020

cuggcccuga agccccucaa gaccaccaag cugacguucu ccaagcagaa cugcccccug     4080

accaaggacg cgaucuucua cuaccagagc ccgaccggga ugaucacccg caaggagucc     4140

aucaaggaca uccucaagca gaacaucuga                                      4170


<210>  2077
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2077
augagccgca agaacaucgu ggaggacuac gccaucuccc uggacaucgg caacgccagc       60

gucggguggg uggcguucac ccccaacuac cggcucgugc gcgccaaggg ccacgagcug      120

aucggggccc ggcuguucga gccggccucc accgccgagg agcgccggau ggcgcgcacg      180

acccggcgcc gguacagccg ccggcgcugg cggcuccgca ugcuggacuc cauguucgac      240

caggcccuga gcgaggucga ccccuccuuc cucaugcggc gcaaguacag cugggugcac      300

cccgaggaca agaacaacga ggcccacugg uucggcgggg ugcuguucga cuccaacgag      360

caggacaagc gguucuacga gaaguacccc accaucuacc accuccgcaa gagccugaug      420

gaggacgacg agcggcacga cauccgcgag gucuaccugg ccauccacca caugaugaag      480

uaccggggca acuuccugac cgagggcgag cucgcgccgu ccagcguguu cgacguggag      540

gacuuccuga agcugcucac ggagauccug caguacgccg acgaggggga gaccgacgag      600

gaccuggagc uccaggccga cgccaacggc cugaccgagg cccugugcac cacgaagggg      660

ucccgcagca uccgcgcgaa gaacgccauc uccauccuca aggagggcgc cgcccugagc      720

aagcggcagg aggccaucau cuccgcgguc uucgccgggc uggagggcaa caagcucgac      780

cugaccaagg uguuccccaa caaggaguac acccccgagg acaagaagac ccuggcccuc      840

uacuucaaca aggccgacua cgacgagaag cgcguggcca ucggcgacag cgggcugcug      900

gaggacaacg agugcgacuu ccucgacugc auccggcgcc aguacuccgc gaucgcccug      960

aagcagcugc ucggcgacuu cacgagcauc agcgagucca ugugcgcccg guacgagcag     1020

caccagcgca acugggaccu gaucaagcgg uacugccgcg aggagagcaa cgccgagaac     1080

aucaacaaga acuacggggc ccuggucggc ugggagaucg ugggcgggca gcggcgcucc     1140

auccggggcg cgaaggacua cgagaacaag cgcaaggccg ccaacaaguu cuucgaggag     1200

cucaucaagg ggagcgcccu gcccgaccag gagaaggagc ggcugcucca cgacaucgag     1260

aacgacgagc uguucccggu gcagcgcgac uccggcaacg gggucauccc cuaccagcug     1320

caccucaacg agcugaagaa gaucauccag aagcagggcc gguacuaccc cuuccugcuc     1380

gagaccuaca ucaaggacgg caaggaggug aacaagcugg aggggcugcu cgcguuccgc     1440

gugcccuacu acgucggccc gcuggugagc cgggacgaca ugcaguccag cgacaaccgc     1500

gagaaccacu ggaugauccg gcgcaagaag ggggugauca cccccuggaa cuucgacgag     1560

auggucgaca aggacaaguc cggccacgag uucaucaaga gccugguggg gaccgacucc     1620

uaccugcucg gcgagacgac ccugcccaag aacagccugc ucuaccagga guucgaggug     1680

cugaacgagc ugaacaacgu ccgccucucc auccggaccg gcaaccacug ggccgacaag     1740

cgccgggugc gccuggggca caacgagaag aagcugcucc uggaggagcu guucgccaag     1800

caggccaccg ucacgaagaa ggccgcggag aaccuccugc ggcgcaccua cggcaacacc     1860

uacgagauca gcgggcuggc cgacgagaag aaguucgugu ccagccucuc cagcuacggc     1920

aagaugugcc ggacccugaa ggccuccuac gugaacagcc acaaggacgu cguggagaag     1980

aucaucgagc ugcagacggu guucgaggac aagaagaccc ucaagcacca gcugcagcug     2040

cucggggagc uggccgagac caacugcgag cugcucagca acacccacua cacgggcugg     2100

ggcaagcugu cccgcaagcu gcucaccagc aaggucgugg ugugcaagau cgggaacgac     2160

uucgcccccg cgaagcacuc caucaucgag aucaugcggg agaccgaccg caacuucaug     2220

gagaucauca ccgacaagga ccugggcguc ggggagugga ucaaccagca gaacgugggc     2280

gccgaggacg gcaacagcua ccaggacauc aucgacgacc ugcggguguc cccgaagguc     2340

aagcgcggga ucaaccaggc cauccgggug aucgacgaca ucagcaaggc cgugggcaag     2400

gagcccuccc gcaucuuccu cgagcuggcc ggggacgucc agcagagcgc gcggacggug     2460

ucccgcaaga gccggcugca gggccucuac aaguccgccg ggcugcagaa ggaguuccgc     2520

gaccuggccg agagccucaa cgagugcucc gacaacgacc ugcgggacga ccgccuguuc     2580

cucuacuaca cccagcuggg caaggacaug uacaccggcg agccccugaa ccucgaccgg     2640

cugagcuccg acuacgacau cgaccacauc aucccccagg ccgugaccca gaacgacagc     2700

cuggacaacc gcguccucgu ggcgcgcgug gccaacgccc ggaagacgga cuccuuccag     2760

uaccugccgg agaucgccaa ccgcaugcgg aacuucuggc aggagcugcu cgacgccggg     2820

cugaucagcc gcgucaaguu cgaccggcug aaccgccaga acgacuucag cucccacgag     2880

aaggagcggu ucgugcagcg cagccucgug gagacccggc agaucaugaa gaacgucgcg     2940

accaucaugc gcaagcggua cggcaacucc gccgccguga ucgggcugaa cagcgagcug     3000

accaaggaga ugcgccagua ccucggcuuc ucccacaaga accgggacau caacgacuac     3060

caccacgccc aggacgcccu gugccugggg aucgcgggcc aguucgccgu gaaccgcggc     3120

uucuucgcca acgggauggu cagcgacggc gccgugaacu ccuacaaccu cuaccugcag     3180

gacuaccugc ggggguaccg cgagaagaug agcgccggcg agcggcgccg gaacaaggcg     3240

uucggguuca ucgugggcuc caugaacagc caggacuccg acaagcgcau caaccccaag     3300

accggcgaga ucgccuggga cgacgaggac aaggucuacc uccgcaaggu gaugaacuac     3360

cggaagaugc ugaucacgca gaaggugggg gacagcuucg gcgcccugua caacgagacc     3420

cgcuacggga ccuccgccaa ggagggcguc aacgggaucc ccuucgacaa gcacaagcag     3480

gacaccgcgc ucuacggcgg cuucagcucc gccaagacgg uguacguggu ccugaucgag     3540

gccaagaaga agacccggcu ggugaacauc accaugcagg aguacgccga gcucggggac     3600

caccccagcg acgacgcccu gcgcgcggug cuggccaaga agaagccgga guacggcaag     3660

gcccggaucc uccugcgcca caucccgccc augcagcuga uccgguacgg gggcgcccuc     3720

gucaccguga aguccgccac ggagcugaac aacgcgcagc agcuguggcu ccccuacaag     3780

gaguacugcc uguucgacga cgugaucagc uccgccaucg acgucgacga ggcccgccug     3840

ugccagaugc ucgacgugau ccuggacgcc auccagaagu acuaccccaa gcagcgguuc     3900

gacgagaccg gccugagcgc cuuccacaac gcguucaacg aggugagcgc cgaggacaag     3960

aagcgcaucg ucggggagau ggugggcaug cuccacgccg acgccaagaa cgccaaccug     4020

aaggcgcugg ggauguccgg ccgguggggg cgcaugagca accaguccgg cuacaccuuc     4080

gccgacagcg acgaguucau cuuccagucc cccagcggcc ucuucgagaa gcggaucucc     4140

gugggggagc ugaagaagcg ccgggcccgc uga                                  4173


<210>  2078
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2078
augaaggagg ugaaggacua caucaucggc cuggacaucg ggaccaacag cugcggcuac       60

gucgugaccg acaagcagaa caacauccuc aagcugaagg ggaagacggc caucggcgcc      120

cgccuguuca aggaggggga ggcggccgcc gaccggcgcg ccuuccggac cacccgccgg      180

cgccucgccc ggcgccggug gcgccugggc cugcucgagg agaucuucga cgaggagaug      240

gcgaaggugg acuccagcuu cuuccggcgc cugaaggagu ccgacuacag ccccaaggac      300

ucccggaagc aguucaacag caucgucuuc gaguccagcg aggccgacaa ggacuucuac      360

gacaaguacc cgaccaucua ccaccuccgc aacgcccuca ugcacgacga ccagaagcac      420

gaccugcggg agaucuuccu ggccgugcac cacaucguga aguaccgcgg caacuuccuc      480

cgggaggacu ccgucaaggc guucaaggcc gccaaguuca cgcugcaggg ggaggacggc      540

aucgggcccg uggacaagcu gaacgaccuc cugaaggaga ucuacaucga ggccgccccc      600

gagcuggcga ccgacaaccu cagcaagauc gaggagauca ucaaggacaa gaagcuguac      660

aagcaggaca agcugaagca ggucgccacc cuccugacca aggaggccga cacgaaggac      720

aaggccaagc ugaacaagga cguggccaag caggucgcga acgccuucau gggcuacaug      780

aucaucuucg acaccaucuu caaccucucc gacguggaca gcaaggacua caaggugaag      840

uucuccgacg ccaacaucga cgagaagcug gacacccuga ccagccuccu gacggacaag      900

cagaccgagu ucgugcugga gcuccagaac aucuacaaca ccaucgugcu gaacgagauc      960

guccccgacg ggaagucccu gagcgagucc auggugaaga aguacgacga ccacaaggag     1020

gaccucaagc uguucaaggc cuaccugaac agccucaccg acgugaagaa ggucaaggag     1080

cugaaggccg cguacgugcu guacgugaac aaccgcaagg ccaaccuccu ggccgccaag     1140

aagcugcucg gcaagaaggc cgacaacaug aacaacuucg agaucuucuc caaguucguc     1200

gcggacaacc uggacgacag cgagcuggcc aacaagaucc gggcccgccu cgaccugggc     1260

gaguuccugc cgaagcagcg gacgaaccag aacgggguga uccccuacca gcuccaccag     1320

guggagcuga cccagauccu ggagaagcag ggcaaguacu accccuuccu gaucgccccc     1380

aacccggucg agagccaccg caacaacgcg cccuaccgca ucuccgagcu cgugagcuuc     1440

cggguccccu acuacguggg gccccugauc gacccgaaca acgccgaguc cgagcaggac     1500

aaggccaaca gcuccuucgc cuggauggug cgccagaagc agggccagau cacccccugg     1560

aacuucgagg agauggucga caccacggag agcgccaaca aguucaucaa gcggaugacc     1620

accaaggaca ccuaccugcu cggcgaggac gugcugcccg cguccagccu gaucuaccag     1680

aaguucguga uccucaacga gcugaacaac aucaagguca acgggaucaa gcugacgucc     1740

gaggagaagc accagaucuu cgacgagcug uucaaggugc agaaguccgu gagccugaag     1800

aaccugguca acuuccucac cgcccacggc ugcguggccc ccaacgugac cgggcugagc     1860

gacggcacca aguucaacuc cagcuacucc acguacaucg acuaccgcaa gaucuucggg     1920

gccgagaucg acaacaagga caagcaggcc gacuucgaga agaugaucga guggagcacc     1980

gucuucgagg accggaagau cuucaagcgc aagcuggagg agaucaccug gcucaccccg     2040

gaccagauca agcaggucuc cagcaagcgg uacuccggcu ggggccgccu gagcaagaag     2100

cugcucacgc agaucaccga cgagaacggg gugaacaucc ugcagcggcu guggaacgag     2160

agcgagaacc ucaugcagau ccuguccgac cccgugauca aggagaagau cguggcggcc     2220

aacagcuccu ucaccaaggc caccgacaug gaggacaucc uggccgaggc cuacacgagc     2280

ccccagaaca agaaggcgau ccgccaggug augcgggucg uggacgacau caucgccgcc     2340

gcccacggca agaagcccuc ccagaucgcg aucgaguucg cccgcgagga ccaggacaac     2400

ccgcagauga ccaagagccg gaaggcccag cucgaccaga ucuacgccaa gaucuccgac     2460

gaguuccugg acaacgccgu gaagaacgag cugaagaaca ugaaggacaa caagccccuc     2520

gcgaaggaca aggucuuccu guacuucaag cagauggggc gcgacgccua caccggcgag     2580

aagcugagcc ucgacaaccu gcaggacuac gacaucgacc acaucuaccc ccaguccuuc     2640

aucaaggacg acagcuucga caaccgggug cuggugcagc gcgccgucaa caacgggaag     2700

uccgacaacg ugcccgugcu ccuguucggc aacaagcagg ccgccgaccu gggcaugacc     2760

auccggcaga uguggaagaa guggcucgag cuggggcuga ucagcaagcg caaguucaac     2820

aaccucauca ccgacccgga gaagaucucc agcuacaugg cguccggcuu cgucaaccgg     2880

cagcuggugg agacgagcca ggugaucaag cuggucgcca acauccucca ggccaagcug     2940

cccgacaccg agaucaucga ggugaaggcc uccuacaaca gcauccugcg caaggaguuc     3000

aaccucuaca agagccgcga ggugaacgac uaccaccacg ccaucgacgc guaccugacc     3060

accaucgucg ggaacuaccu guaccaggug uaccccaagc uccggcccua cuucguguac     3120

ggccaguuca agaaguucgg gaaggccgag uccaaggagg acgacccggu caagaagauc     3180

aagcaguuca acuucaucua ccagcugcug uggggcaagg acgacgccau cugcaucagc     3240

cacacggacc agcaggucuu cucccgcagc gacaucauca acaagcucac caccgccuac     3300

aacuucaagu acaugaacgu gucccaggag guguacacca acagcaacaa cguguucaag     3360

cagacggugu uccccaucau cgagcgggac accgcgaagu cccgcagccu gauccccaag     3420

aagcuggacc ggccgaccga caucuacggc ggguacucca acaacaacga cgccuaccuc     3480

gcccugguca agguggacaa gaagaagggc agcgaguacc gcguggucgg gauccccaug     3540

cgggccgccg cgaugcugaa ggccuccaag aacuacgacg aggaccuccg caaggugauc     3600

gagcccaugg ugaugcugga caagaacggc aaggccaagc ggggcaucga gggguuccgc     3660

guccuggugg gcaagaugcc cuucaagcag gccgugcucg acggggacaa gaaguucaug     3720

cugaccggca cgaacgacau gccgaaccug aagcagcucg uccugagcca gcagaacaug     3780

aaguacaucc uggacuacau cgacgacauc gacugccgga agcacaaguu cgggaacaag     3840

acccaggacg gcaacaagug ccuccuggag guguucgacg agaucaucca gaucgucgac     3900

aagcacuucg agcuguucga caaccgcucc uuccgggaga agcuccgcaa cagccgggug     3960

aacuucauca accuggcccu guccaagaag gcggaggccc uccuggccau ccugaacggc     4020

cuccacgcca acagcaugcg caccaccauc aaggagcuga agaucuccga ccuggggcgg     4080

auccagagcu ccgugaucag ccccgaggcc cagcucaucu accagagccc cacgggccug     4140

uucgagcgcc gggucaaggu gaaggaccug uga                                  4173


<210>  2079
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2079
auggagaaca agcaguacua caucggccug gacgugggga ccaacagcgu cggcugggcc       60

gugaccgaca cguccuacaa ccuccugcgc gccaagggga aggacaugug gggcgcgcgg      120

cuguucgaga aggccaacac cgccgccgag cgccggacca agcgcaccag ccggcgccgg      180

uccgagcgcg agaaggcccg gaaggcgaug cucaaggagc uguucgccga cgagaucaac      240

cgcguggacc ccagcuucuu cauccggcug gaggagucca aguucuuccu cgacgaccgc      300

agcgagaaca accggcagcg cuacacgcug uucaacgacg ccaccuucac cgacaaggac      360

uacuacgaga aguacaagac caucuuccac cuccgguccg cccugaucaa cagcgacgag      420

aaguucgacg uccgccuggu guuccuggcg auccucaacc uguucuccca ccgggggcac      480

uuccugaacg ccagccucaa gggcgacggc gacauccagg ggauggacgu guucuacaac      540

gaccuggucg aguccugcga guacuucgag aucgagcugc cgcgcaucac gaacaucgac      600

aacuucgaga agauccucag ccagaagggc aagucccgga ccaagauccu ggaggagcug      660

agcgaggagc ucuccaucag caagaaggac aaguccaagu acaaccugau caagcugauc      720

agcgggcucg aggccuccgu gguggagcug uacaacaucg aggacaucca ggacgagaac      780

aagaagauca agaucggcuu ccgcgagagc gacuacgagg agagcucccu gaaggucaag      840

gagaucaucg gggacgagua cuucgaccuc guggagcggg ccaagagcgu gcacgacaug      900

ggccugcugu ccaacaucau cggcaacagc aaguaccucu gcgaggcccg cgucgaggcg      960

uacgagaacc accacaagga ccugcugaag aucaaggagc uccugaagaa guacgacaag     1020

aaggccuaca acgacauguu ccgcaagaug accgacaaga acuacuccgc cuacgugggg     1080

agcgugaacu ccaacaucgc caaggagcgg cgcagcgucg acaagcggaa gaucgaggac     1140

cuguacaagu acaucgagga caccgcccuc aagaacaucc ccgacgacaa caaggacaag     1200

aucgagaucc uggagaagau caagcugggc gaguuccuca agaagcagcu gacggcgucc     1260

aacgggguga uccccaacca gcugcagagc cgcgagcucc gggccauccu gaagaaggcc     1320

gagaacuacc ugcccuuccu caaggagaag ggcgagaaga accugaccgu guccgagaug     1380

aucauccagc uguucgaguu ccagaucccg uacuacgucg gcccccucga caagaacccc     1440

aagaaggaca acaaggccaa cagcugggcc aagaucaagc agggcgggcg cauccugccc     1500

uggaacuucg aggacaaggu ggacgugaag gggucccgga aggaguucau cgagaagaug     1560

guccgcaagu gcaccuacau cagcgacgag cacacccugc cgaagcaguc ccugcucuac     1620

gagaaguuca uggugcugaa cgagaucaac aacaucaaga ucgacggcga gaagaucagc     1680

guggaggcga agcagaagau cuacaacgac cuguucguca aggggaagaa ggugucccag     1740

aaggacauca agaaggagcu caucagccug aacaucaugg acaaggacuc cguccugagc     1800

ggcacggaca ccgugugcaa cgccuaccuc agcuccaucg gcaaguucac cgggguguuc     1860

aaggaggaga ucaacaagca gagcaucguc gacaugaucg aggacaucau cuuccugaag     1920

accguguacg gcgacgagaa gcgguucgug aaggaggaga ucgucgagaa guacggggac     1980

gagaucgaca aggacaagau caagcgcauc cugggcuuca aguucuccaa cugggggaac     2040

cucagcaagu ccuuccugga gcuggagggc gccgacgugg gcacggggga ggugcggagc     2100

aucauccagu cccucuggga gaccaacuuc aaccugaugg agcugcucag cucccgcuuc     2160

accuacaugg acgagcugga gaagcggguc aagaagcugg agaagccccu cagcgagugg     2220

accaucgagg accuggacga cauguaccug uccagccccg ugaagcgcau gaucuggcag     2280

uccaugaaga ucguggacga gauccagacg gucaucggcu acgcccccaa gcggaucuuc     2340

guggagauga cccgcagcga gggggagaag gugcggacca agucccgcaa ggaccggcuc     2400

aaggagcugu acaacggcau caaggaggac agcaagcagu gggucaagga gcuggacucc     2460

aaggacgaga gcuacuuccg cagcaagaag auguaccucu acuaccugca gaaggggcgc     2520

ugcauguacu ccggcgaggu gaucgagcug gacaagcuca uggacgacaa ccuguacgac     2580

aucgaccaca ucuacccgcg gagcuucgug aaggacgacu cccuggacaa ccucguccug     2640

gugaagaagg agaucaacaa ccgcaagcag aacgacccca ucacgcccca gauccaggcc     2700

agcugccagg gcuucuggaa gauccugcac gaccaggggu ucauguccaa cgagaaguac     2760

agccggcuca cccgcaagac ccaggaguuc uccgacgagg agaagcugag cuucaucaac     2820

cggcagaucg uggagaccgg ccaggcgacc aagugcaugg cccagauccu gcagaagucc     2880

augggggagg acgucgacgu gguguucagc aaggcccgcc ucgucuccga guuccggcac     2940

aaguucgagc uguucaagag ccgccugauc aacgacuucc accacgccaa cgacgcguac     3000

cucaacaucg uggugggcaa cuccuacuuc gucaaguuca cgcggaaccc cgccaacuuc     3060

aucaaggacg cccgcaagaa cccggacaac cccguguaca aguaccacau ggaccgguuc     3120

uucgagcgcg acgucaagag caaguccgag guggccugga ucggccagag cgaggggaac     3180

uccggcacca ucgugaucgu gaagaagacc auggccaaga acagcccccu gaucaccaag     3240

aaggucgagg aggggcacgg cuccaucacg aaggagacca ucgugggggu gaaggagauc     3300

aaguucggcc ggaacaaggu cgagaaggcg gacaagaccc ccaagaagcc gaaccugcag     3360

gccuaccgcc ccaucaagac cagcgacgag cggcucugca acauccugcg cuacggcggg     3420

cggaccagca ucuccaucag cggcuacugc cugguggagu acgugaagaa gcgcaagacg     3480

auccgguccc ucgaggccau ccccgucuac cuggggcgca aggacagccu guccgaggag     3540

aagcuccuga acuacuuccg guacaaccug aacgacggcg ggaaggacag cguguccgac     3600

auccgccucu gccugcccuu caucagcacc aacucccugg ugaagaucga cggcuaccuc     3660

uacuaccugg gcgggaagaa cgacgaccgc auccagcugu acaacgccua ccagcucaag     3720

augaagaagg aggaggucga guacauccgg aagaucgaga aggccgugag cauguccaag     3780

uucgacgaga ucgaccgcga gaagaacccg gugcugaccg aggagaagaa caucgagcug     3840

uacaacaaga uccaggacaa guucgagaac accgucuuca gcaagcggau gucccucgug     3900

aaguacaaca agaaggaccu gagcuucggc gacuuccuga agaacaagaa guccaaguuc     3960

gaggagaucg accucgagaa gcagugcaag gugcuguaca acaucaucuu caaccugagc     4020

aaccucaagg aggucgaccu guccgacauc gggggcagca aguccacggg gaagugccgc     4080

ugcaagaaga acaucaccaa cuacaaggag uucaagcuga uccagcagag caucaccggc     4140

cucuacagcu gcgagaagga ccugaugacc aucuga                               4176


<210>  2080
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2080
augaaggaga ucaaggacua caucaucggc cuggacaucg ggaccaacag cugcggcuac       60

guggucaccg acaagcagaa caacauccuc aagcugaagg ggaagacggc caucggcgcc      120

cgccuguuca aggaggggga ggcggccgcc gaccggcgcg ccuuccggac cacccgccgg      180

cgccucgccc ggcgccggug gcgccugucc cugcucgagg agaucuucga cgaggagaug      240

aacaaggugg accccagcuu cuuccggcgc cugaaggagu ccgacuacag cccgaaggac      300

cggcgcaagc aguucaacuc caucguguuc gagagcuccg aggcggacaa ggccuucuac      360

gagaaguacc ccaccaucua ccaccuccgg aacgcccuga ugcacgacaa ccagaagcac      420

gaccugcgcg agaucuuccu ggccguccac cacaucguga aguaccgggg caacuuccuc      480

cgcgaggaca gcgugaaggc guucaaggcc gccaaguuca cgcugcaggg cgaggacggg      540

aucggccccg ucgacaagcu gaacgaccuc cugaaggaga ucuacaucga ggccgccccc      600

gagcuggcga ccgacaaccu cuccaagauc gaggacauca ucaaggacaa gaagcuguac      660

aagcaggaca agcugaagca gaucgccaac cuccugccga aggccgugga cagcaaggac      720

aaggccaagc ugaacaagga caucgccaag caggucgcga acgcccucau gggguacaug      780

uuccggcugg acacccuguu cuccuucacc gacguggacg ugaaggacua caagcucaag      840

uucagcgacg ccaacaucga cgagucccug gacgcccuga cgagccuccu gaccgacgcc      900

cagaucgagu ucgugcugga gcuccagucc aucuacaaca ccaucguccu gaacgagauc      960

gugcccgacg gcaugagccu guccgagagc auggugaaga aguacgacga ccacaagaag     1020

gaccucaagc uguacaagga guacaucgac ucccugagcg accagaagaa ggcgaagcag     1080

cucgaggccg ccuacgcccu guacgucaac uaccgcaagg ccgaccugcu cgcggccaag     1140

aagcuguucg agaagaagaa gaucggggac aagaaguucg uggacgugau cagcaacuuc     1200

gaggucuucg gcaaguucgu guccgacaac cuggacgaca gcgagcucgc caacaagauc     1260

aaggcccggc uggaccuggg cgaguuccuc cccaagcagc gcaccaacca gaacggggug     1320

auccccuacc agcugcacca ggucgagcug acgcagaucc uggagaagca gggcaaguac     1380

uacccguucc ucaucacccc caaccccgug gagucccacc gcaacaacgc gcccuacgag     1440

aucagcgagc ugguguccuu ccgggucccg uacuacgugg ggccccugau cgacaaccag     1500

agcaucaagg acaagcagaa caagaacaag uucgccugga ugguccgcca gaagcagggc     1560

cagaucaccc ccuggaacuu cgaggagaug guggacacca cggaguccgc caaccaguuc     1620

aucaagcgga ugacccgcaa ggacaccuac cuccuggccg aggacgugcu gcccaagagc     1680

ucccucaucu accagaaguu caugauccug gacgagcuga accggaucaa gaucgacggc     1740

aagaagcuca ccagcaagcu gaagcacgac aucuucgaga agcuguucaa gaagcagaag     1800

uccaucaacc ucgacaaccu gaagaacuac cugcucgccg aggggaacau cccgggccug     1860

aucgaggggc ugagcgacgg caucaacuuc aacaacuccu ucagcacgua caucgacuac     1920

cgcaacaucu ucggggacga gaucgacaac cccaacaagc aggcggacuu cgagaagaug     1980

aucgaguggu ccaccgucuu cgaggaccgg aagaucuuca agcgcaagcu caaggagauc     2040

accuggcuga cccccgagca gaucaagcag gucagcucca agcgguacag cggcuggggc     2100

cgccugucca agaagcuccu gacgcagauc accgacgaga acggggugaa cauccugcag     2160

cggcucugga acgagcccga gacccugacc gaggugcugg ccaacccggu gaucaagcgc     2220

aagaucagcg aggccaacag ccucuucgug cagaucaaca aggucgagaa cauccuggac     2280

gacgccuaca cgagccccca gaacaagaag gccauccgcc aggugauccg gguggucgac     2340

gacaucaucg cggccgccca cggcaagaag cccagccaga ucgccaucga guucgcccgg     2400

uccagccaga acaaggcgaa ggugcccgac acccgcaaga agcagcugga caagaucuac     2460

aacaagaucu ccagcgagau ccucgacucc agcaucaaga acgagcugaa gaaccugaag     2520

uccaacaagu accucagcaa ggacaagcug uuccuguacu ucaagcagau ggggcgggac     2580

gccuacaccg gcgacaagcu cucccuggac cagcugcaga acuacgacau cgaccacauc     2640

uucccgcgca gcuucaucaa ggacgacucc cucgacaacc gggugcugac ccagaagccc     2700

aucaacgcca agaaguccga cuacgggauc cccgcccugg aguucggcaa caaguacguc     2760

cccgaccucg gcaucacggu gaaggagaug uggaagcugu ggcaggagaa cgggcugauc     2820

uccaagagca agcucaucaa ccugugcacc aacccgaaga agaucggcuc caagcgcgcg     2880

agcggguuca ucaaccgcca gcugguggag accagccagg ucaucaagcu cguggccauc     2940

auccugcagg ccgaguuccc cgacaccgag aucaucgagg ugaaggcccu gcagaacacg     3000

acccuccggg aguccuucca ccuguacaag aaccgcagcg ucaacgacua ccaccacgcc     3060

aucgacgcgu accugaccac caucgugggc aacuaccucu accaggugua ccccaagcug     3120

cggccguacu ucgucuacgg gcaguucaag aaguucaacc aggagaagaa caucgacauc     3180

cugaagcgcc ucaagaacuu caacuuccug cggcagcuga ucuucaacac cgacgacaac     3240

aucuacaucu ccggcacgaa ggagaucgug uucaacaaga aggacaucau ccacaagcuc     3300

gagaccgccu acggcuacaa guacaugaac aucagccgcg agugcuacca ggagaccucc     3360

agccuguuca accagacccu guacgcccac aacuccagcg ugaagaacuc ccucaucccc     3420

aagaagaagg ggcugcccac ggagaucuac ggcggguaca gcggcaacaa ggacuccuuc     3480

uucguccugg ugaagaucgu gaagaagcgg accaaccucu accgcaucgu cggcaucccc     3540

acccgggagc uggccaagcu gaacagcucc aacaacuaca accaggcccu caacaagauc     3600

guggagagca agcugugccu gaaggagacc gaguccuuca agauccucau caagcgccug     3660

cuguacggga cgcucaucgu ggacaacggc cagaaguucc ggaucgggag cuucaaggag     3720

aagcacaacg uccagcagcu ggugcugcag cucaagucca ugaaguacau caaguucuac     3780

aucgacggcg ggcagaacua cuucaccgac guggagcgca agaagcugga gaagcaggac     3840

cgggacaagu gccugcucua cgucuucgac gacaucauga acguggugaa caagcgcuuc     3900

acccuguucg acaugagcaa guacgagaag gacggcgacu cccugcggga gaaguucaac     3960

ugccucgacu ucaacgacaa ggucagcauc cugagcgacc ugcucaaggc guuccacgcc     4020

aacuccgacc gcaccagcau cacgaagcug aagaucacca accugggccg gcaccaggcc     4080

gggaagaacg gcaucacccu caccacgaac gcccagauca ucuaccaguc cccgaccggg     4140

cuguucgagc gccggaucaa gaucaaggac cuguga                               4176


<210>  2081
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2081
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgagc acgagcacga gcaguccagc aucgucaagc ucggggugcg cgugaacccc      120

cuguccgucg acgagaagac caacuucgag aagggccggc cgcugagcau caacgcggac      180

cgcacgucca agcggagcgc ccgccggaac cuccagcgcu ucaagcugcg gcgcaagaac      240

cugaucaacc uccugcugaa gcaccacauc aucuccaacg agaccauccu caccgaggug      300

gggaagaaca ccacgcacca gacccugaag cugcggagca aggccaccca ggagaagauc      360

ucccucgagg aguucgcccg cauccugcug agcaucaaca agaagcgggg cuacaagucc      420

agccgcaagg agaagaacga ggacgagggc uucaccaucg acgggauggc caucgcgaag      480

gagcucuacg acaaccagcu gacgcccggc caguacgugu acaacauccu gcaggacggg      540

aagaacuaca uccccgacuu cuaccggucc gaccuccagg ccgaguucga cgacgucugg      600

caguuccaga agaaguucua cagcuccauc cugaccgacg aguucuaccu gaagcucaag      660

ggccagggga aggagaacac ccgcaagcug uuccuggcca uccaccaggu guacaccgcc      720

gagaacaagg gcaagcggga cgcggugaag cuccaguacu acaaguggcg cagcgaggcc      780

gucaagaagc agcuguccau cgaggaggug gccuacgugc uggucgagau caacaacaac      840

aucaacaaga gcuccggcua ccucggggcc aucagcgacc ggagcaaggc ccuguacuuc      900

aacaacgaga cggugggcga guaccuguac gggcagcucg ugaagaaccg ccacaacucc      960

cugcggaacc aggucuucua ccgccaggac uaccuggacg aguucgagaa gaucugggag     1020

acccaggcga aguucuacga cgagcucacc aacgagcuga agcaggagau ccgggacgug     1080

gugaucuucu accagcgcaa gcucaagagc cagaagcacc ucaucuccga cugccuguuc     1140

gagaagcacc acaaggccau ccccaagagc uccccgcugu uccaggaguu caagaucugg     1200

cagaaccuca acuaccugga guucguccac acccagagca aggagaagau cuucuucucc     1260

gagcuggacg acgagacgcg ccaguucgug uucgccgagc ucaacauccg gggcacccug     1320

aagaucaagg agaucaagaa gcugaucggc cucgagaccc ccguguacug gaaguccaac     1380

uucgacgaga ucgaggggaa ccgcaccaac cagguccugu acaacgugua ccagaacauc     1440

gccgacgacc ugggcuacgg guucgacugg agcaagaagc acgccuucga gaucaacgcg     1500

gagcugcagg ccaucuucaa ggacgugggc aucgacacgg ccauccucaa cuucgacgcc     1560

agcaucgagg agaacgacuu cgacaagcag aaguccuacc agcuguggca ccuccuguac     1620

agcgccgagg acaccgacaa ggcgaccgag gaggacaagc ugaucuacgg gaacaucaac     1680

accaagcuga agaagcagcu ccaccagaag uacggcuucg aggccaagua cggcaaccug     1740

cuggccaaca ucucccucca gcaggacuac gggaaccuga gcgccaaggc caucaagaag     1800

auccugcccu accucgaguc cggcaacagc uucuacgagg cgugcacgau cgucggguac     1860

aaccccucca acagccugac caaggagcag cagcagaacc ggaagcugaa ggacaagcuc     1920

gagcugcugc cgaagaacuc ccuccgcaac cccguggucg agaagauccu gaaccagaag     1980

gugaaccuga ucaaccagcu caucgacgag uacggcaagc ccgacgaggu gcggaucgag     2040

cuggcccgcg agcugaagaa guccgccaag cagcgggccg accucagcaa gcacaucaac     2100

gacgcgaccc ugaagaacca gaacauccgc aagacccugc agaaggacuu cgggauccag     2160

aaccccagca agaacgacgu cauccgguac aagcucuacg acgagcugaa ggacaacggc     2220

uacaagacgc uguucaccaa caaguacauc ccgaaggagc agcuguucag caaggacgug     2280

gacaucgagc acaucauccc caaggccauc cuguucgacg acuccuucag caacaagacc     2340

cuggccuacc gcaacgugaa ccucgccaag gccgacgcga ccgccuucga cuucaucucc     2400

agcaagcacc uggacgaccu ggacaacuac aaggcccggg ucgagacccu cuuccagucc     2460

aagcgcauca gcaagggcaa guacaagaag cugcugauga cccggcagaa ccuccccgag     2520

gacuucaucg agcgcgaccu gcggaacucc caguacaucg ccaagaaggc caccgcgaug     2580

cugaaggagg uguuccgcag cguggucccc accuccgggc ggaucacgga caagcuccgc     2640

gaggacuggg accuggugaa cgugaugaag gagcugaacc ucccgaagua ccggacccug     2700

ggccugaccg aguucaucga gcgcaagaac gggaacaaga ucgagcagau caucgacugg     2760

accaagcgca acgaccaccg gcaccacgcc auggacgccc ucaccgucgc cuucacgacc     2820

cacaaccaca uccaguaccu gaacaaccug aacgugaccc acggccucaa cgggguggac     2880

accgaccacg agaagucccg ccuguacgcc aucaagcagg cgaucacgaa ggucuacauc     2940

gugggcaaga ccaagaagcg gaaguucauc ccgcccaugg agaacuuccg cagccacgcc     3000

aagaaccacc uggagggcau ccucaucagc uucaagaaca agaacaaggu ggucaccaag     3060

aacaagaacc ggaccaagac gaagaccaag gggguguuca acgagaagau ccagcugacg     3120

ccccgcggcc agcugcacaa ggagaccauc uacgggcacg ccaagaaggu gaucgucacg     3180

aaggagaagg uguccagcaa guucacccac gagaagaucc uccaggugac caagcccgcc     3240

uaccgggagg cgcugcugaa gcgccucgcc uccuucaaga acgaccccaa gaaggccuuc     3300

agcggcaaga acgcccugag caagaacccc cuguacuucg acaucaccuc cggcaagaag     3360

guccccgaca cggugaagcu cagcuacuuc gagaacuccu acgugauccg gaaggagguc     3420

aacagcgaca acuucaagaa cgagaagucc cugcagaagg ugcuggacgu ggggauccgc     3480

cggauccucg agaagcgccu ggccgacuac aacggcaacg cgaaggaggc cuucagcaac     3540

cuggagaaga acccgaucug gaaggacaag gagaaggggg ucugcaucaa gaaggugcgg     3600

aucgaggccc ucaagaacgc cgagccccug cacgugaaga aggaccaccu gggcaagauc     3660

auccucgacg acgagaacaa guccaccccc gucgacuuca ucagcaccgg gaacaaccac     3720

cacguggcca ucuacgagga caaggacggc aagcugcacg acaagguggu cuccuucuac     3780

gaggugguga gccgcgucaa ccugggccuc cccaucaucg acaaguccuu caacagccac     3840

cuggacugga aguuccuguu cuccaugaag cagaacgaga uguucguguu cccgagcgag     3900

gacuucaacc ccaaggagau cgacaucggg aaccccaaga acaacgagcu caucuccaag     3960

uaccuguucc gggugcagaa guucagcaag gucaccuacg gcaacuccgc ggugcgcgac     4020

uacguguucc ggcaccaccu ggagacggag cucaucgaca agaaggagcu gaaggggauc     4080

accuacuacc agcucaagag caucggcaug cuggagaagc uggucaaggu gcgcaucaac     4140

cacaucggga agaucgugca caucggcgag uacuga                               4176


<210>  2082
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2082
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaag       60

gaggccgaga acgagaacga ggucuccgac aucaucaagc ucggggugcg cgugaacccc      120

cugacggucg acgagcugac caacuucgag ggcgggaagg cguacagcaa caacgccgag      180

cggacccuca agcgcggcgc ccggcgcaac cugcagcggu acaagcugcg ccgggagaac      240

cucaucgaga uccugcugaa gaacggccuc aucaacaagg acaccgcccu gacggaggug      300

gggaaggaga ccacccacca gacccugaag cuccgcucca agagcgccaa cgagaaggug      360

ucccuggagg accuggcgaa gauccuccug agcaucaaca agaagcgggg cuacaagucc      420

agccgcaagg ucaacgccga ggacgacggg guggccaucg acggcaugga gguggccaag      480

cugcucuacg acgagaacau gacgccgggg gaguacgucc ugcggcugcu cgaguccggc      540

aagaagcacc uccccgacuu cuaccgcagc gaccugcagg aggaguucaa ccucgugugg      600

aagaagcagu ccgaguucua cccccugauc cugaccaagg agcuguucuu cgagcugcac      660

ggcaagaaca agagccagac cugggcgauc ugcaaggagc cccuggggau cgugggcguc      720

aagcucaagg gguccgccaa ggagcagaag uucgcccggu accgccugcg gaaggaggcc      780

cugacccagc agaucgagcu cgagcaccug gccaucgugc ugcaggacau caacaacgac      840

agcaacaagu ccagcggcua ccucggcgcg aucuccgacc gcagcaagga gcuguucuuc      900

aacaaggaga cgguggggca guaccaguac aagcagcugc agaaggaccc gaacaccucc      960

cucaagaacc aggucuucua ccggcaggac uaccuggacg aguucgaggu gaucuggcgc     1020

aaccaggcca aguucuaccc ccagcugacc aacgagcuca agcaggagau ccgggacgug     1080

gucaucuucu accagcgcaa gcugaagucc cagaagaacc ugaucagcuu cugccaguuc     1140

gaguccaccc agcagaacua cguggagaag gagacgggca agaccaagau ccggaccauc     1200

gggcgccgcg ugaucccccg gagcagcccc cucuuccagg aguucaagau cuggcagagc     1260

cugaacaacc ugaaguucgu caacuccgag agcaaccaga ucauccaguu caucgagcug     1320

gaggacgacg ugaagaaggu ggucuucgac gagcucaacc ugcgcggcga ccugaagccg     1380

aaccaggugc ucaagauccu guccaaguac augaagaucg ggaagcugac ccaguggaag     1440

ugcaaccucg aggagaucga gggcaaccgg acgaacagcg cccuguacaa cgucuuccag     1500

augaucgccg aggaggaggg cuacggguuc aacuggggca agaaguccgc ccuggagauc     1560

aagagcgagc ucaaggcgau cuucuccagc aucgggauca acgacuccau ccuggacuuc     1620

gacuucagca agaagcugga ccagcagcuc uccuaccagc uguggcaccu ccuguacagc     1680

gccgaggagg gcaacuccaa gaucaccgag gaggacaagu ucaccuacgg gaacaccggc     1740

guggcccuga agaagaagcu gcaccucaag uucggcuuca agcccgagua cagcacgcug     1800

aucuccaacc ugcgcuucac cagcgacuac gggaaccuca gcuccaaggc caucagcaag     1860

auccugcccu uccugcggga cggcuccgac uacagcaagg ccugccagua cgcgggguac     1920

aaccacucca acagccucuc cgccgacgag cuggacaacc gcgagcugaa gcccaagcuc     1980

gagcugcuga agaagaacag ccuccggaac ccgguggucg agaagauccu gaaccagaug     2040

gugaacgugg ucaaccaggu gaucgacaac uacggcaagc ccgacgaggu gcgcaucgag     2100

cuggcccggg agcucaagaa guccgccaag gagcgcagcc agaugaccaa gaacaucucc     2160

gacgccaccc ggcgcaacgc ggagaucaag aaguaccuga gcaaggaguu cgggaucccc     2220

aaccccacga agggcgacgu cauccgguac cgccuguggg aggagcucgc cuccaacggc     2280

uacaaggacc uguuccggga caaguacauc ccgaaggaga agcuguucag caacgagauc     2340

gacaucgagc acaucauccc caaggcccuc cuguucgacg acuccuucag caacaagacc     2400

cuggccuuca agguggacaa ccucaagaag gcggaccgca ccgccuucga cuucaugucc     2460

accgacuuca gcaaggacgu ggacagcuac aucgggcgga ucgagacgcu gcacaaggac     2520

ggcaagaucu ccagcuccaa guacaagaag cugaugaucc ccaagagcaa gcucgucgac     2580

ggguucaucg agcgcgaccu gcggaacacc caguacaucg ccaagcaggc ccgcaagaug     2640

cugcucgagg uguucaagac cguggucccc accacgggca agaucaccga caagcugcgc     2700

gaggacuggg accugaucaa cgugaugaag gagcucaacu ucccgaagua caaggcccug     2760

ggccugacca agaagaucga gcgguucgac gugggguccg agaagaucaa gagcaucaac     2820

gucaucgagg gcugguccaa gcgcaacgac caccggcacc acgcgaugga cgcccucgcc     2880

guggccuuca ccacccacaa ccacauccag uaccugaacu accugaacgc ccgcaaggac     2940

auccaccacg agaugcacgg gacgaucaag agcaucgaga accagaucau cggcaaggac     3000

gugaugggga agcggcgcuu caucccgccc aucuccgacc uccggaugaa ggcgaagcug     3060

cagaucgaga gcauccugau cuccuucaag aacaagaaca aggucgugac cuggagcgug     3120

aacaagacca agcucaaggg caaggacaac uacaacaagc gccggcagcu gacgccccgc     3180

ggccagcugc acaaggagac cgucuacggg aagaucaagc ucaucgaccc caagccccug     3240

aagcugaaca acaaguucac ccucgugcgg gccuccauga ucguggacaa gguccaccgc     3300

gagaucgugc ugcagcaccu gagcaaguac gacaacaacu ccgagguggc cuucgacagc     3360

aagaccauca agaucgaccc cauccucauc gagggcaucg ggcugaagga ggucgugugc     3420

uacgaggacg uguucaagau ccggaagaag aucuccggcg acaacuucaa gaacgagaag     3480

caccuggaga aggucaucga cgagaaggug aagcagaucc ucaaggaccg cgugaucaag     3540

uuccagggga acuucaagga ggccuucagc aaccuggagg agaaccccau cuggcugaac     3600

aaguccaagg gcaucgccgu caagagcgug gcgaucaagg gcgugaacaa cgccgagagc     3660

cuccacgagg ggaaggacca cuucggcgac cagauccugg acaagaacaa gaagcagauc     3720

ccggucgacu ucguguccac cgggaacaac caccacgugg gcaucuacga ggacaaggac     3780

gggaaccugc aggagcgggu cgugagcuuc uucgaggccg ugacgcgcgu caacgagggc     3840

cuccccauca ucgacaagac cuacaacucc gacaucggcu ggaaguucaa guucaccaug     3900

aagcagaacg agauguucgu guuccccagc gacgacuucg acccgaacga guucgaccug     3960

accgacggga agaacgccgc gaagaucucc cccaaccugu accgggugca gaagaucgcc     4020

acgaagaacu acauguuccg ccaccaccuc gagaccgucg ugaccaacga ccuggaguuc     4080

accuacaaga gcauccgguc cacgggcugg cugcgcaacc ucgugaaggu ccggaucaac     4140

caccuggggg agaucgugua cgucggcgag uacuga                               4176


<210>  2083
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2083
augaagaaga uccugggccu cgaccugggg accaccagca ucggcugggc ccuggugaac       60

gagaaggaga acaacgagga gcucuccagc aucauccgcc uggggguccg ggugaacccc      120

cugacggugg acgagaaguc caacuucgag aagggcaagg acaucaccac caacgccgac      180

cgcaccaagu gccggagcgc gcgccggaac cuccagcgcu ucaagcugcg gcgcgaggcc      240

cuggccgacu gccucaagcg gcacgggcug augacggacg agaccccgcu gugcgagacc      300

aacaacucca ucaucuucaa gaccuacagc aaccgcgcca aggccgucug cgagaagauc      360

gcgcucgacg acuucgugcg ggugcuguuc augaucaaca agaagcgcgg cuacaagucc      420

agccggaagc ugaacacgga cgacgagggc acccucgucg acgggaugga cguggccaag      480

cugcuguaca acgagcagau cacccccggc gaguaccucc ugcgccuguu caagaagggg      540

aagaagaccc ggcccgacuu cuaccucucc gaccugaagg ccgagcugga gcgcaucugg      600

gacuuccagc ggacguucua ccccgagauc cucugcaacg cccugaagga ggaccugaag      660

ggcaagagcc gcacccaguc cagcgcgauc uucuacgcca aguacaaggu guccaccgcc      720

aagaucgccg ggaaggacaa gcggcuccag gagcugaccu ggcgcacgga cgcccugcgg      780

aagcagcuca ccaucgacga ggucgcguac gugaucugca gcaucaacgg cgaggugaac      840

ggcuccagcc agcgccuguc caugaucagc gaccgcucca agcugcucua cuucaaccac      900

cagaccaucg ggcaguaccu gaugcagcgg cuggaggaga acccgaacgc cggccucaag      960

aacaucaucu ucuaccgcca ggacuacgag gacgaguuca acgacaucug gaacagccag     1020

gccaccuacc acaaggagcu gacgcccgag cugaagaagg aggucaagga caucaucuuc     1080

uaccagcggg ggcucaagag cugcaagugg cugaucaacu acugcgagcu ggagcacgag     1140

gagaucgagg ugaagacccc cgagggcaag cuccgcaaga ucaccaucgg cuccaagacc     1200

ugccccaaga gcuccccgcu guuccaggag uucaagaucu ggcagguccu gaacaaccuc     1260

caggugagcc cccugaacuc ccggcgcaag gccaagcgga agaaggacga ggagcccagc     1320

cuguucuccg acgaggacau gaacacggcc ugccgcuacc ucaacgagga ggagaagcug     1380

acccuguuca aggagcugag caucaagaag gagcucaaga agaaggacgc gcugaagcuc     1440

cuguucgaga acuacaagga gcuggaccug aacuacaagg ugaucuccgg gaacgugacc     1500

auggccaacc ucuaccgggc cuuccaggac aucaucagcc ugaccggcaa cggggaguuc     1560

gacuuccagg ccaugcccgc cgaggaggcg gagaagacgg ucgaggagau cuucggcggg     1620

cugggcuaca agaccgccuu ccuccacuuc gacgccuccc ugcacgacaa ggaguucgag     1680

cgccagccgg ccuaccggcu guggcaccuc cuguacagcu acgccggcga ccaguccaag     1740

accgggaucg aggcgcuggu gaagcgcauc caggagcucu gcggcuucga gcccgaguac     1800

gcccggaucc uggccaacau cagcuucauc cccgacuacg ggaaccuguc cagcaaggcc     1860

augcgcaaga uccuccccua caugcaggac ggcuacgagu acuccgaggc gugcagcaag     1920

gccggguacc ggcacuccgc cagcagccug accaaggagg agaucaacaa gaagaacuac     1980

aagacgcacc uggacgagau caagcgcaac ucccuccgga acccgaucgu ggagaagauc     2040

cugaaccaga uggucaacgu ggugaacagc gucaucgaca ccuacuccaa caucgacgag     2100

auccgcaucg agauggcccg ggagcugaag aaguccaagc ccgagcgcga ggacaugagc     2160

uucgccauca gcaaggcgac caaggagacc gagcgcauca agggcauccu cgagcaggag     2220

uucaagaucc agcacgugac gcggaacgac aucauccgcu accggcugua cgaggagcug     2280

aagcccaacg gcuacaagac ccucuacucc aacaccuaca ucccccgcga ggaccuguuc     2340

agcaagaagu ucgacaucga gcacaucauc ccgaaggccc ggcuguucga cgacuccuac     2400

agcaacaaga cccucgagcu gcgcgacauc aacaucgaga aggggaacca gaccgccuac     2460

gacuucgucc uguccaagua cggcgaggag gggcucuaca aguacgagca gcggguggag     2520

gaguucaaga agaacaacga gcaggccggc acgaagugcc gcaagcugcu gaugcgggag     2580

gccgacaucc ccagcgacuu ccucaaccgc gaccugaacg acucccagua caucgcgaag     2640

aaggcccacg agcugcuccg ggagaucgug cccaccguca ccgccaccac ggggagcauc     2700

accaaccgcc ugcgggagga cuggaagcug gugaacguga ugaaggagcu caacugggag     2760

aaguacgaca agcugggccu gaccgaggag uacaaggaca aggacggcaa caccguccgc     2820

cacaucaagg acuggacgaa gcggaacgac caccgccacc acgccaugga cgcccucacc     2880

aucgcguuca ccaagcccuc cuucauccag uaccugaaca accugaacgc ccggagcaac     2940

cgcgggggcu ccaucuacgg gaucgagcag aaggagcucg agcgggagaa caacaagcug     3000

guguucaucc cgcccaucga ccccaagauc uuccgcaagc aggccaagga gcaccugagc     3060

aacauccuca ucagcaucaa ggccaagaac aaggugguca ccaagaacgu gaacaagacg     3120

aaguccaaga gcggcaccaa ccggaaggug cagcugacgc cccgcggcca gcugcaccuc     3180

gagaccgucu acgggaagau ccgcaccuac aagaccgagg agaucaaggu gggcaccaag     3240

cugaccgagg agcugaucgc gaagguggcc gacaagaaga uccgggaggc ccucaugcag     3300

cgccuguccg aguacggggg caacaucaag aaggccuucc agaacaucag caagcacccg     3360

aucuggcuga acggguccca cacgaagcac guccccgaga aggugaagau cguguucuuc     3420

gaggacgucu ucaccauccg gaaggccauc acccccgaca ucaagaucga caaggugauc     3480

gacagcaaga uccgccggau ccuccaggag cgccugaacg aguucggcgg cgaggcgucc     3540

aaggccuuca gcaaccugga cgagaacccg aucugguuca accgggacaa ggggaucgcc     3600

aucaaguccg ugaagauccg cggcaucgcc aaggggaccg ccauccgggu caagaaggac     3660

auccacggca agacgaucau cgggaacaac ggcuaccccg uggcgaacga cuacgugaac     3720

accggcaaca accaccacgu cgccaucuuc cgcgacaagg acgggaaccu ccaggagcac     3780

aucgugagcu ucuacgaggc caccgcccgg gccauccaga accugcccau cguggaccgc     3840

aacuacaacu ccgagaagga cuggaaguuc cuguucagca ugaagcagaa cgaguacuuc     3900

gucuucccca acccgaacac cggcuucaac cccuccgaga ucagccucaa ggacgagaag     3960

aacuacgcgc ugaucucccc caaccuguuc cgggugcaga agcucagcca cuacuacuac     4020

guguuccgcc accaccugga gacgaacguc gacaccaacc ugcagcucaa ggacaucacc     4080

uggaagcgga ucaccgccgu gaacaaccug acgggggccg ugaagguccg caucgaccac     4140

cugggccgga ucguggacgu gggggaguac aaguga                               4176


<210>  2084
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2084
augaagaacu accacaucgg ccuggacauc gggaccagcu ccgugggcuu cgccgcccgc       60

gacgacgaca acaaccucau ccgggucaag gggaagaacg ugaucggcgc gcgccuguuc      120

agcgaggggc agaccgccca ggagcggcgc auggcccgga ccucccgccg gcgcuacaac      180

cggcgcaagu ggcggcugcg ccuccuggac gagaucuucc acgacgagau cauccacaag      240

gacgccgagu ucuucaaccg gcugaagcag agcuuccucu cccccaagga cgagcgcaag      300

caguacuucg gcagccugcu guucccggag accggcgacu ccgaguacua ccacaacggg      360

aaccagacca ucuaccaccu ccggcaccgc cugaucaccc aggacgagaa ggccgacauc      420

cgggagaucu accuggcgcu ccgccacauc aucaaguacc ggggcaacuu ccuggacgac      480

acgcccguga gcuccuucga ggccagcgac cugaagcucg ccuccuucgu ccccaccauc      540

aacgaccugu ucgagaccau cgacgugaac uacgaguuca gcauggacaa caucaacgag      600

aucgaggaca uccugcucaa caacaagcug cgcaacuccg acaagagcaa gcagcugacc      660

gaccuccuga cggagcccau cgugaacauc gacgacaagu cccuggagaa gcaguucaac      720

aaggagcgga ccgacaucag caaggagauc gccaaguccg ucguggggag caagaugaac      780

gugaacaagc uccuguccuu cgagagcagc gagggcucca agaucgcguu cagcuucucc      840

gacgccagcu ccgaggacaa gaugauggag cugagcgaca uccucgacga cucccgcaug      900

aacauccuga acauccugaa ggucaucuac agccgcgugc ggcucaacca gaucaucccg      960

uccgggaugg gccugagcga guccauggug gccgccuacg acagccacaa gcagcagcug     1020

aaggaccuca agacccugcu gaacguccuc cccuucaacg agcgcuacgc cgugaagauc     1080

gcguacgccg ccuacaucgg caacaucgac aagcagaagu ucaccaaguc cgagaucaag     1140

gccaucgaca agcugaagga gaaguccgcc aucaugaacg ugcuggacuu caaguccggg     1200

cacaucacga acgaggagcu cagcaacguc aucaaggcgg ugaucgugga caaguaccug     1260

aacgacgagg acuucgagua ccacggcauc gucgagaaga ucaaccagga caucuccaac     1320

gggagcuacc ugcucaagca gcggaacaac cagaacggca acauccccca ccagcugcac     1380

cagauggagc uggaccagau caucgagaac caguccaagu acuaccacuu ccucggcgag     1440

cugaacccca acagcaaccg caaccacauc gccaaguaca agaucagcga gcugguggcc     1500

uuccgggugc cguacuacgu cgggccccuc aucacccccg aggagcagga gaagaccucc     1560

ggcgccagcu ucgccuggau gaagcgcaag aaggacgggg cgaucacccc cuggaacuuc     1620

gaggagaagg uggacaagga ggagacggcc ucccgguuca ucaagcgccu gaccgugaag     1680

gacaccuacc ugcugaacga ggacguccuc ccggacaaca gccugcugua cgagaaguuc     1740

aaggugcuca acgagcugaa cgucgugaag gccaacaaca agcggcugac cguggaggag     1800

aagcagggcg ccuucgacaa ccucuucaag aagaugaaga cggucaccgc caagcgccug     1860

aagaacuacc ugguguccaa ccacaaccac aucaacagcg uggagaucac cgggcucucc     1920

gacggcgaca aguucaacaa cagccugucc accuacaucg acuucaagaa gaucuucggc     1980

gacaagaucg acaaccccgg gcugcucgac gacuucgagg acaucaucga guggagcacg     2040

gucuucgagg accggaagau cuucgaggag aaggugcgca aggacaucuc cuggaucagc     2100

gaguccgaga ucaagagcgu ggucuccaag cgguacagcg gcugggggcg ccuguccaag     2160

aagcugcuca acugcaucac caacaacgag ggccggaaca ucaucgacga gcugugggag     2220

gagagcgaga ccuucaugga ggcggugagc aagcccgagg ugaaggagca gaucgacggg     2280

aucaacuccg acuucaucaa gaacaccgac aucgagagcg uccuggccga cgccuacacg     2340

agcccccaga acaagaaggc cauccgcgag acccacaagc ucgugaagga cauccagaag     2400

gcgaugggcg gccaggcccc ggccagcauc uccaucgagu ucacccggaa cccccaggac     2460

aaggggcagc ugacccgcag ccggcacucc gaggugagcc gcaaguacaa ggagaucucc     2520

aaggagauca gcaagacgcu gaacgacgag cucaagucca agaagaacag cauggacguc     2580

gacaaguacu accuguacuu caugcagaug ggcaucgacc uguacgacgg guccagcauc     2640

gacaucgacg accucaacaa cuaccagauc gaccacauca ucccccaguc cuucuacaag     2700

gacgacagcu ucgacaaccg cgugcugacc cacuacucca acaaccagaa gaagggcaac     2760

aacacgcccc ugaacgggcu caacaucaac gacaagaccc gggccaucug gaaccaccuc     2820

cgcgaccugg gccugaucac caagaagaag uacaacggcc ugacccugaa ggggauggac     2880

cagcucagca aguacaccaa gcacggcuuc auccggcgcc agcuggugga gaccucccag     2940

gucaucaagc ugguggccaa cauccucagc gacgaguuca gcaccgacga cacgaagauc     3000

aucgagguga aggcgaagau gaacucccag auccggcagg ucuucaaccu guacaaggug     3060

cgcgagcuga acgacuacca ccacgccgug gacgccuacc ucaccaccuu caucgggggc     3120

uaccuguaca accgguaccc gaaccugcgc aacauguucg ucuacggcga cuucaagaag     3180

uucagcgccg acguggacaa ccucaagacc uucaacuucc ugcacgagau cacgaacccc     3240

uccgacaagu ucgaggaccg gaucuucgac gagaaguccg gggagcugau ccucgaccgc     3300

aagcacgcca ucgagaccau caagaagauc uaccaguaca aguacaugcu gaucacccgg     3360

gaggugagca ccaagaacgg ccagcugagc gaccagacgg ucuacuccgc gaagcgcauc     3420

aacaacguga aggcccccau ccccgugaag aaggacuuca acccggagcu cuacggguuc     3480

cggaccggca acgucgacca ccacauggug aucgugaagu accccaagaa ggacagcuac     3540

gacuacaagg ucguggggau cccccugcgc uucgugcaga agcugaagga gaccaaggac     3600

aacaaggacu acgagaaccu ccugaaccag aucguccaga ugaagcugaa ccggaagaag     3660

caggacuuca ucgugguggu cgacaacgug auguacggcc agcucaucca ggacggcggg     3720

cagaaguuca cccugggcuc cagcacguac cgcuacaacg ccaagcagcu ggugaucgac     3780

aacggguacc ucaagcugau cgccgacgag cgguacaaga acucccugac cgacgaggag     3840

aaguccaacc aguacaucac ccucuacaac gagauccuga aggucgugaa cggccgcuuc     3900

gacguguucg acgucaacag cuuccggaag aagcugaaca acggggugga caaguucauc     3960

aagcucaaca ccguggacaa gcgcaucauc aucaugaaga uccugagcgg ccugcacugc     4020

ggccccucca ucgucccgcu caaggagcug gggcugacga cgccccucgg ccagcugcag     4080

agcaccuccg ggaucagccu guccgagaac accaucauca ucuaccagag ccccagcggc     4140

cucuucgagc ggaaggugcg ccugggggac cuguga                               4176


<210>  2085
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2085
augaagaacu accacaucgg ccuggacauc gggaccagcu ccgugggcuu cgccgcccgc       60

gacgacgaca acaaccucau ccgggucaag gggaagaacg ugaucggcgc gcgccuguuc      120

agcgagggga agaccgccca ggagcggcgc aucucccgga ccagccgccg gcgcuacaac      180

cggcgcaagu ggcggcugcg ccuccuggac gagaucuucc acgacgccau cgugcacaag      240

gacgccgagu ucuucaaccg gcugaagcag uccagccucu cccccaagga cgagcgcaag      300

caguacuucg gcagccugcu guucccggag gucggcgacg gggacuacuu ccgggacggc      360

aaccagacca ucuaccaccu ccgccaccgg cugaugaccc aggacgagaa ggccgacauc      420

cgcgaggugu accuggcgcu ccggcacauc aucaaguacc gcgggaacuu ccuggacgac      480

acccccgugu ccagcuucga ggccuccgac cugaagcucg ccagcuucgu ccccacgcug      540

aacgaccugu ucgagaccau caacgugaac uacgagcucu ccaucgacaa cgugaaccag      600

aucgagaaca uccugcugaa caacaagcuc cggaacagcg acaaguccaa gcagcugacc      660

gaccugaucg ccgagcccgu caccaacauc gacgacaaga gccucaagaa gcaguucgac      720

aaggagcgca aggacaucuc caaggagauc gcgaaggccg uggugggcag caagaugaac      780

gucaacaagc ugcuguccuu cgagagcagc gacgggucca agaucagcuu cuccuucagc      840

gacgccucca gcgaggacaa gaugcucgag cuguccgaca uccuggacga cagccgcaug      900

aacauccuca acgugcugaa ggugaucuac ucccgggucc gccugaacca gaucaucccg      960

agcggcaugg gccucuccga gagcaugauc gccgccuacg accugcacaa gcagcagcug     1020

aaggaccuca agacgcugcu ggacguggug cccuucaacg agaaguacgc ggucaagauc     1080

gccuacgccg ccuacaucgg gaacaucgac aagcagcggu ucaccaaggc cgaggugaag     1140

accaucgaca agcuccgcga caagaagucc aucaccagcg ugcuggacuu caaguccggc     1200

cacaucacga acgaggagcu gaacaacguc aucaaggagg ugaucgugga caaguaccuc     1260

aacgacgaga ccuucgagua ccacgacauc gucgagaaga ucaaccagga caucagcaac     1320

gggaacuacc ugcugaagca gcggaacaac cagaacggca acauccccca ccagcuccac     1380

cagauggagc uggaccagau caucgagaag caguccaagu acuaccccuu ccugggcaag     1440

cucaacccga acagcaagcg cucccacauc gcgaaguaca agaucagcga gcugguggcc     1500

uuccgggucc ccuacuacgu ggggccccug aucacccccg aggagcagga gaagaccagc     1560

ggcgccuccu ucgccuggau gaagcgcaag gaggacgggg ccaucacgcc guggaacuuc     1620

gagaagaugg uggacaagga gagcaccgcg ucccgguuca ucaagcgccu gaccgucaag     1680

gacaccuacc uccugaacga ggacgugcug cccgacaaca gccuccugua cgagaaguuc     1740

aaggugcuga acgagcucaa cgucgugaag gccaacaaca agcggcugac gguggaggag     1800

aagcagggcg ccuucgacaa ccuguucaag cagaugaaga ccgucaccgc caagcgccuc     1860

aagaacuacc ugguguccaa ccacaaccac aucaacagcg ugaagaucac cgggcugucc     1920

gacggcgaga aguucaacaa cagccucucc gucuacaucg acuucaagaa gaucuucggc     1980

gacaagaucg acgacagcaa gcuguucgac gacuucgaga acaucaucga gugguccacg     2040

guguucgagg accgggacau cuucgaggag aaggugcgca aggacaucag cuggaucucc     2100

gagagcgaga ucaacgccgu cguguccaag cgguacagcg gguggggccg ccuguccaag     2160

aagcuccuga acgggaucac caacgacgag ggccggaaca ucaucgacga gcugugggag     2220

gagagcgaga ccuucaugga ggcggugagc aagcccgagg ucaaggagaa gaucgacgag     2280

aucaacucca acuucaucaa gaacaccgac aucgagagcg ugcucgccga cgccuacacg     2340

agcccccaga acaagaaggc cauccgcgag accuacaagc uggugaagga cauccagaag     2400

gcgaugggcg ggcaggcccc gagcuccauc agcaucgagu ucacccggaa cccccaggac     2460

aagggcgagc ugacccgcuc ccgccacagc gagaucgcca agaaguacaa ggagcucucc     2520

aaggagguca gcaagaagcu gaacgacgag cugaagucca agaagaacag cauggacgug     2580

gacaaguacu accucuacuu caugcagaug gggaucgacc uguacgacgg cacguccauc     2640

gacaucgacg accugaacaa cuaccagauc gaccacaucg ugccccagag cuucuacaag     2700

gacgacuccu ucgacaaccg gguccucacc cacuacagca acaaccagaa gaaggggaac     2760

aacacgcccc ugaacggccu gaacaucgac uccaacaccc gcgaggugug gaaccaccuc     2820

aucgggcugg gccugaucac caagcggaag uacaacaacc ucacccugaa gggcauggac     2880

cagcugagca aguacaccaa gcacggguuc auccgccggc agcucgugga gaccucccag     2940

gucaucaagc ugguggccaa cauccugagc gacgaguaca gcaaggacga caccaagauc     3000

aucgagguga aggccaagcu caacucccag auccgccagg ucuucaaccu guacaagauc     3060

cgggagcuga acgacuacca ccacgcgcuc gacgccuacc ugacgaccuu caucggcggg     3120

uaccuguaca accgcuaccc gaaccuccgg aacauguucg uguacggcga cuucaagaag     3180

uucagcgcca agaucgacaa ccuggagacc uucaacuucc ugcacgacau caccaacccc     3240

uccgacaagu ucgaggaccg caucaucgac gagaaguccg gcgaggugau ccucgaccgg     3300

cgccacgcca ucgagacgau caagaagauc uaccaguaca acuacaugcu gaucacccgg     3360

gaggucucca ccaagaacgg gcagcugagc gaccagaccg uguacuccgc ccgccggauc     3420

aacaagguga acagccccau ccccgucaag aaggacucca acccggagcu cuacggcuuc     3480

cgcacgggga acguggacca ccacauggug aucgucaagu accugaacaa ggacaaguac     3540

gacuacaaga ucaucggcau cccccugcgg uucguccagg ugcucaagaa cagcaacgac     3600

aacaccgagu acaagaacau ccugaacaag aucgugcagu ccaagcugaa gggcaagagc     3660

accgacuucc gcgugauccu cgacaacgug auguacgggc agcugaucca ggacggcggg     3720

cagaaguuca cccugggcuc cagcacguac aaguacaacg cgaagcagcu cgucaucaac     3780

auggacgaca uccggauccu gguggacaac cgcuacuuca acucccugag cgacgacgac     3840

gccuccgggg aguucaucaa gaucuacaac gagauccuca aggugaucaa cgcccgguuc     3900

gaccuguucg acaucaacaa guuccgcgag aagcugaaca acggcaagga guacuucgag     3960

aaccucagca ucgacgagaa gaaccagguc cugcugaaga ugcucuccgg gcugcacugc     4020

ggcagcgcca acgugcccau caaggagcug ggccucacca cgccccuggg gaagcugcag     4080

aucccgagcg gcaucgugcu cuccgaggac gccaucguca ucuaccagag ccccuccggg     4140

cuguucgagc gcaagauccg gcugaaggac aucuga                               4176


<210>  2086
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2086
augaaccugc gcaacuaccc cggcgaguac agcaucgggc ucgacauggg caccgggucc       60

gugggcugga gcgucgugga cgagaagggg aaccugcugc acuucaagaa gaagccgacc      120

uggggcuccc ggcuguucga cagcgugaug cccgccuccg aggcccgcgu ccaccggggc      180

cagcgccggc gcuacgagcg gcgccggugg cgccuggacc ugcuccaggg gcuguucaag      240

aacgagauga acaaggugga ccccgaguuc uucaugcggc ugaaccagag ccgcacgauc      300

gagggcgacc ccaucuucac caaguccuuc accaaggcgg acuacuacga ccgguucccg      360

accaucuacc accuccgcgc cuaccugaug gagagcaacg agcccgccga ccugcggcuc      420

aucuaccugg ccaugcacaa caucaucaag caccgcggga acuuccugcg ggaggaggag      480

aagucccuca gcgccaagca cgcggacucc aagaaggccg ugacgcaguu cgucgacgcc      540

cugaaggagu ggugcuucag ccgcggcuac gaguucggga aggaccccgc caaguccauc      600

gagaagugcc ugagcgacuc caagagcucc cggagccagc ucgcguccga gaucgccaag      660

gcccuggccg ugagcaccga cgccgcggcc gccgacaaga agcuggccga caaccucgcc      720

aaggcgaugg ugggccugca gugcgaguuc aaguacgucu ucggcgaguu cgacugcgag      780

agcaccaagc uggcccucuc cgacgacgag aagcuggagc ugcuccgcgc cgccugcccc      840

gacgacagcg uggagcugcu ggacgcccuc ugcgcggugu acuccgccua cguccugcag      900

gggcugcuca gcuacgcccc gggccagacc aucucccaca acaugaucgc caaguacgac      960

cuguacaaga cggaccugaa gacccuccgg gcccuggugc gcgaguacgc gcccggggcc     1020

uacgacgagu ucuuccgcgg cgccaccuac cacgacgcca accccgacga cagcuccaag     1080

gacuacagcu acgcggacgc cgagggcuac accgccuaca accugcacaa gcucuccuac     1140

gacgacuucg ccaaggccgu gaagaagcug cucgggggca cggacgcggu caccgacgag     1200

cgguaccuca acaugaugga gcgcuucgag aagcagcagu uccugcggcg ccugaagacc     1260

agcgacaacg gggccaucua cuaccagcuc caccuggagg agcugaccgc caucaucaag     1320

aaccagggcc gguucuacuc cuuccucaag gaguacgagg ggaaguucga gagccuggug     1380

uccuuccgca uccccuacua cgugggcccg cugacgagcg ucaacgccgc ccaggacucc     1440

cacaaccaca accgguucca guggagcgag cgcaagcccg gcauggagcg ggaggugauc     1500

acccccugga acugggacac caucaucgac aagaacaagu ccgcggagaa cuucaucaug     1560

cgcaugaccg gggacugcac guaccuggcc ggcgagaagg ugcuccccaa gcagagccug     1620

cuguacgagg aguucugcgu ccucaacgag cugaacgggg ugcggugguc cgacgacggc     1680

gacgacuggc agcgcuucga cgccgcccag cgggagggga ucgugcgcga gcuguuccac     1740

aaguaccggc gcgucaccua caagaugcuc gaggacuggc uggugcggga gggcgacgcc     1800

aaccacccga ucgucaaggg cgggcagggc gagaccgggc uggagagcaa gaugggcagc     1860

uaccucuucu ucgcgaaggg gaucuucggc cugggcgagc ugucccccgc cgaguacccc     1920

gccaucgaga agaugaucaa guggaacacc cucuucgagg accgcagcau ccugaaggag     1980

aagcuggagg acgaguucgg ggagcggggc aacgggaugc ucgacgccuc ccagaucaag     2040

gcgaucugca agaagcgcuu cacgggcugg ggccgccuga gcgagcaguu ccugaccggg     2100

cucaaggugg agucccccgc cggccgggcc gugagcauca uggacguccu gcgcgagggg     2160

aacccgugcu ccgagcggcg ccagggcgag accgugguga ugauggaggc ccugcgggac     2220

gaggggcucg gcuuccagaa gaagaucgac gagcacaacc gcaacuacua cggccagcac     2280

gggaaggacc ugggcgucaa cgagcugccc gggagccccg ccauccggcg cucccucaac     2340

caggcgaucc ggaucgugga ggagaucgcc agcaucgccg gccacgcccc cgccaacauc     2400

uucaucgagg ugacccgcgg ggaggacgcg ggcaagaagg gcaagcggac ggucaagcgc     2460

ugggacaaga ucggggaggc ccugaagaag cugaaggagg agggcguggc cguggagguc     2520

auggacgacu ucaaggagca gaaggccauc aacgccaacc ucgacgagcg gcugucccug     2580

uaccucaugc agaacgggaa gugccuguac agcggcaagg cgaucaaccu ggagaagcuc     2640

cuggccgggu ccggcgagua cgagguggac cacaucaucc cgcgcgccua caucaaggac     2700

gacagcaucg agaacaaggc ccuggugcuc cgggaggaga accagcacaa gaccgaccag     2760

cugcugaucg acaaguccau ccgcuggaag augcgggaga ccuggagcca gcuccacgac     2820

gcgggccuga ucggggacaa gaaguaccac aaccugcucc gcuccaacau caacgagaac     2880

cagcugcggg gcuucaucgc ccgccagcug gucgagacca gccagauggu gaagcucgcc     2940

caggcccugc uggaggcccg guucgcgaac gaggagacgc gcaucgugcc cgucaaggcc     3000

agcauguccc acaaccuccg cgaggccgcc gaccuggcca agugccggga ggcgaacgac     3060

uuccaccacg cccacgacgc cuuccuggcc agccgcaucg gccucuucgu gcagaugcgg     3120

caccccggga ucuacgagaa ccccaucggg uacucccacg ugaucaagaa guacguccag     3180

gagcagagcg agguguucaa gaagacccac cgcaugccgg gcuccgccgg cuucgugauc     3240

aacagcuuca uguccagcgg guucgacaag gacaccggcg aggucuucaa ggacuccugg     3300

gacgcggagg ccgaggugga cgggauccgg aaggcccuga acuaccgcca gugcuacauc     3360

agccggaugc cccagcagga caccggcgcc uucuggaacg ccacggugua cagcccccgc     3420

gacccgaaga ugggccccaa gcuggcgcuc uccaccaagc aggggcugga ccccaagcug     3480

uacggcgggu ucuccagcca gcaguucgcc uacuucuuca ucuacgaggc caagaagaag     3540

ggcaagccca ccuuccgguu cucccagguc ccgguguggc ucgccgcgcg caucgaggcc     3600

aacccccugg cccuggagga guacgcccgg gggcucgcca agggcgaggg ccuggaguuc     3660

gugagcaucc agcgccccaa gaucuacaag aagcagcuga ucgaggucaa cggggacaag     3720

cucaucauca ccggcgcgaa ggaggugcgg uccggggccg agcuggccuu caaccaggac     3780

gagaucagcg ccaucuccau gugcagcaag cugcgcgccg cggaggccgg ccucgacggg     3840

gccuucagcg aggagguguc cgccaucugg aaccggauca gcaacgcccc cguccagucc     3900

ugcggccgcc ugcugcggca gcucacccug ccgaaguacc agagcaacuu cgacuccagc     3960

uccaacgagg agaaggcggc cguggugcug ggccucaucg cccugcugaa cgggaacaac     4020

cgcaucgucg accucagcgg cgucgggggc uccagccacg ccggggcgau gaugaugucc     4080

uaccccaagc ugcuggacga ccccaaggug cccuucuuca ucaucgacca gagcgugacg     4140

ggcauguucg agcggaagac ccgcgugggc cucuga                               4176


<210>  2087
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2087
augaagaaga uccugggccu cgaccugggg accaccagca ucggcugggc ccuggugaac       60

gagaaggaga acaacgagga gcaguccagc aucauccgcc ucgggguccg ggugaacccc      120

cugacggugg acgagaaguc caacuucgag aagggcaagg acaucaccac caacgccgac      180

cgcaccaagu gccggagcgc gcgccggaac cugcagcgcu ucaagcuccg gcgcgaggcc      240

cuggccgacu gccugaagcg gcacgggcuc augacggacg agaccccgcu gugcgagacc      300

aacaacucca ucaucuucaa gaccuacagc aaccgcgcca aggccgucug cgagaagauc      360

gcgcuggacg acuucgugcg ggugcuguuc augaucaaca agaagcgcgg cuacaagucc      420

agccggaagc ugaacacgga cgacgagggc acccuggucg acgggaugga cguggccaag      480

cuccuguaca acgagcagau cacccccggc gaguaccugc uccgccuguu caagaagggg      540

aagaagaccc ggcccgacuu cuaccugucc gaccucaagg ccgagcugga gcgcaucugg      600

gacuuccagc ggacguucua ccccgagauc cugugcgacg cccucaagga ggaccugaag      660

ggcaagagcc gcacccaguc cagcgcgauc uucuacgcca aguacaaggu guccaccgcc      720

aagaucgccg ggaaggacaa gcggcugcag gagcucaccu ggcgcacgga cgcccugcgg      780

aagcagcuga ccaucgacga ggucgcguac gugaucugca gcaucaacgg cgagaucaac      840

ggcuccagcc agcgccucuc caugaucagc gaccgcucca agcugcugua cuucaaccac      900

cagaccaucg ggcaguaccu caugcagcgg cuggaggaga acccgaacgc cggccugaag      960

aacaucaucu ucuaccgcca ggacuacgag gacgaguuca acgacaucug gaacagccag     1020

gccaccuacc acaaggagcu cacgcccgag cugaagaagg agcugaagga caucaucuuc     1080

uaccagcggg ggcucaagag cugcaagugg cugaucaacu acugcgagcu ggagcacgag     1140

gagaucgagg ugaagacccc cgagggcaag cuccgcaaga ucaccaucgg cuccaagacc     1200

ugccccaaga gcuccccgcu guuccaggag uucaagaucu ggcagguccu gaacaaccuc     1260

caggugagcc cccugaacuc ccggcgcaag gccaagcgga agaaggacga ggagcccagc     1320

cuguucuccg acgaggacau gaacacggcc ugccgcuacc ucaacgagga ggagaagcug     1380

acccuguuca aggagcugag caucaagaag gagcucaaga agaaggacgc gcugaagcuc     1440

cucuucgaga acuacaagga gcuggaccug aacuacaagg ugaucuccgg gaacgucacc     1500

auggccagcc ucuaccgggc cuuccaggac aucaucuccc ugaccggcaa cggggaguuc     1560

gacuuccagg ccaugcccgc cgaggaggcg gagaagacgg uggaggagau cuucggcggg     1620

cugggcuaca agaccgccuu ccuccacuuc gacgccagcc ugcacggcaa ggaguucgag     1680

cgccagccgg ccuaccggcu guggcaccuc cuguacuccu acgccgggga ccagagcaag     1740

accggcaucg aggcgcuggu gaagcgcauc caggagcucu gcggguucga gcccgaguac     1800

gcccggaucc uggccaacau cuccuucauc cccgacuacg gcaaccugag cuccaaggcc     1860

augcgcaaga uccuccccua caugcaggac ggguacgagu acagcgaggc gugcuccaag     1920

gccggcuacc ggcacagcgc cagcucccug accaaggagg agaucaacaa gaagacguac     1980

aagacccacc uggacgagau caagcgcaac agccuccgga acccgaucgu cgagaagauc     2040

cugaaccaga uggugaacgu ggucaacucc gugaucgaca ccuacggcag caucgacgag     2100

auccgcaucg agauggcccg ggagcugaag aaguccaagc ccgagcgcga ggacaugagc     2160

uucgccauca acaaggcgac caaggagacg gagcgcauca aggggauccu cgagcaggag     2220

uucaagaucc agcacgugac ccggaacgac aucauccgcu accggcugua cgaggagcug     2280

aagcccaacg gcuacaagac ccucuacucc aacaccuaca ucccccgcga ggaccuguuc     2340

agcaagaagu ucgacaucga gcacaucauc ccgaaggccc ggcuguucga cgacuccuac     2400

agcaacaaga cccucgagcu gcgcgacauc aacaucgaga aggggaacca gacggccuac     2460

gacuucgucc uguccaagua cggcgaggag gggcucuaca aguacgagca gcggguggag     2520

gaguucaaga agaacaacga gcaggccggc agcaagugcc gcaagcugcu gaugcgggag     2580

gccgacaucc ccuccgacuu ccucaaccgc gaccugaacg acagccagua caucgcgaag     2640

aaggcccacg agcugcuccg ggagaucguc cccaccguga ccgccaccac gggcuccauc     2700

accaaccgcc ugcgggagga cuggaagcug gugaacguca ugaaggagcu caacugggag     2760

aaguacgaca agcuggggcu gaccgaggag uacaaggaca aggacggcaa caccgugcgc     2820

cacaucaagg acuggacgaa gcggaacgac caccgccacc acgccaugga cgcccucacc     2880

aucgcguuca ccaagcccag cuucauccag uaccugaaca accugaacgc ccgguccaac     2940

aagggcggga gcaucuacgg caucgagcag aaggagcucg agcgcgagaa caacaagcug     3000

guguucaucc cgcccaucga ccccaagauc uuccggaagc aggccaagga gcaccugagc     3060

aacauccuca ucuccaucaa ggccaagaac aaggucguga ccaagaacgu gaacaagacc     3120

aagagcaagu ccgggaccaa ccgcaagguc cagcugacgc cccggggcca gcugcaccuc     3180

gagaccgugu acgggaagau ccgcacguac aagaccgagg agaucaaggu gggcaccaag     3240

cugaccgagg agcugaucgc gaaggucgcc gacaagaaga uccgcgaggc ccucaugcag     3300

cggcugagcg aguacggggg caacaucaag aaggccuucc agaacaucuc caagcacccg     3360

aucuggcuga acagcuccca cacgaagcac gugcccgaga aggugaagau cgucuucuuc     3420

gaggacgugu ucaccauccg caaggccauc acccccgaca ucaagaucga caaggugauc     3480

aacagcaaga uccggaacau ccuccaggag cgccugaacg aguucggcgg ggaggcggcc     3540

aaggccuucu ccaaccugga ccagaacccg aucugguuca acaaggacaa gggcaucgcc     3600

aucaagagcg ucaagauccg ggggaucgcc aacggcaccg cgauccgcgu gaagaaggac     3660

auccacggga acacgaucau cggcaacaac ggcuacccca uccccaacga cuacgugaac     3720

accgggaaca accaccacgu cgccaucuuc cgggaccgcg acggcaaccu ccaggagcac     3780

aucguguccu ucuacgaggc caccgcccgg gccauccaga accugcccau cguggaccgc     3840

aacuacaaca gcgagaagga cugggaguuc cuguucucca ugaagcagaa cgaguacuuc     3900

gucuucccga accccaacac cggguucaac ccccucgaga ucgaccugcg gaacgaggag     3960

aacuacgcgc ugaucagccc caaccucuuc cgcgugcaga agcuguccca cuacuacuac     4020

guguuccggc accaccugga gacgaacguc gacaccaacc uccagcugaa ggacaucacc     4080

uggaagcgca ucaaggccau caacaaccug accggcgugg ugaagguccg gaucgaccac     4140

cucgggcgca ucguggacgu gggcgaguac aaguga                               4176


<210>  2088
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2088
augaagaagc gcaucgacga cuacauccug ggccucgaca ucgggaccaa cagcugcggc       60

ugggccguga ccgacaagaa gaacaaccug cugaagcucc gggggaagac ggccaucggc      120

ucccaccugu ucgaggaggg gcacaccgcg gccgaccgcc ggggcuuccg caccacccgg      180

cgccggcuga agcgccggaa guggcgccuc cggcugcugg aggagaucuu cgccgagccc      240

auggccgagg ucgacccggg cuucuucgug cgccuccacc agagcugggu gagcccccug      300

gacaagagcc ggaagaagua ccaggccauc gucuucccca cggcgaagga ggaccaggag      360

uacuaccaga aguaccccac caucuaccac cuccgcgacg cccugaugac ccaggaccgg      420

caguucgacc ugcgcgagau cuuccuggcc auccaccaca ucgugaagua ccgggggaac      480

uuccuccagg acaccgccgu gaaggacuuc gaggcgucca agaucgaggu cggcccgauc      540

cugacgaaca ucaacaacgc cuucgccgac aagaucgugg aggggcagga ccugaucgag      600

cucaacgugg ccaacgccgc ggagaucgag gccgucaucc gcggcaagga cgccgacaag      660

acccuguaca agauggacaa ggugaagaag aucgccaagc ugcucaccga cagcaccgcc      720

aaggagcaga agaacguggc gaagcagauc gccaacgcca ucauggggua caagacgcag      780

cuggagacca uccugggcaa ggagaucgac aagaacgaca aggcccagug ggaguucaag      840

cucuccgacg ccgacgacaa gcuggacgcg cugcuccccg accuggacga gagcgaccag      900

accgucgugg ccgagaucga gaagcuguuc uccgccauca cccucagcac gaucguggac      960

gagaacaagu cccugagcca guccaugguc gagaaguaca acaagcacca ccaggacuac     1020

aagcugcuca aggccuacau cgagagccug cccgaccggg cccaggcgaa gaagcugcuc     1080

cuggccuacg accuguacgu gaacaaccgc cacggccacc uccuggaggc caagaccgag     1140

uucaagcagg acuccacccu gaccaaggag gacuucuaca agacggugaa ggacaaccuc     1200

gacgacagcg agcuggccca caagaucgcg caggagaucg ccgccgacaa cuucaugccc     1260

aagcagcgga ccaacuccaa cggggucauc ccguuccagc ugcaccagau cgagcucgac     1320

aagaucaucg ccaaccagag caaguacuac cccuuccugg ccgcggagaa ccccguggag     1380

gaccaccgca agcaggcccc cuacaagcug gacgagcucg ugcgcuuccg ggucccguac     1440

uacgugggcc ccaugaucac cgccgacgag caggagaaga ccuccggcaa gagcuucgcc     1500

uggauggugc gcaaggagca ggggcagauc acgcccugga acuucgagca gaaggucgac     1560

cggcaggaga gcgccaacaa guucaucaag cgcaugacca ucaaggacac cuaccugcug     1620

ggcgaggacg ugcugcccgc gaacucccuc cuguaccagc gguucgaggu ccugaacgag     1680

cucaacaaca uccgcgugaa cgggagccgg aucuccgugg accugaagca gcagaucuuc     1740

aacgaccugu ucgaggagaa gaagaccguc acggagaagg cccucaucag cuaccugaag     1800

cagaaccugc accucccgac cguggagauc aagggccugg ccgaccccac caaguucaac     1860

uccagccugg ccuccuacua ccaccucaag aaccugcacg uguucgacaa ggagcuggcc     1920

gacccccagu accagaagga guucgagaag aucaucgagu acagcuccau cuucgaggac     1980

aagaagaucu uccaggacaa gcuccacgcg gaguucaagu ggcugacccc cgcccaguuc     2040

aaggccauca gcacguggcg ccugcagggg uggggccggc ucucccgcaa gcugcugguc     2100

gagcuccacg acaccaacgg gcagaacauc aucgagcagc ugugggacag ccagaagaac     2160

uucaugcaga ucgugaccga gccggacuuc aaggaggcca ucgcgaagga gaaccagaac     2220

aucacccagg agaacggcgu ggaggagauc cuggccgacg ccuacacguc ccccgccaac     2280

aagaaggcca uccggcaggu cgugaaggug gucgcggacg uggugaaggc cgccagcggc     2340

aaggcccccg cccaguucgc gaucgaguuc acccgcgacc ccgacaagaa cccgcagcuc     2400

ucccacaucc gggggaccaa gcugcugaag gccuaccagg agaccgccgg cgagcucguc     2460

gaccagaacc ugaccaacag ccugaaggag gccaugacgu cccgcaagcu ccugaaggac     2520

aaguacuucc uguacuucau gcagaccggg cgggacgccu acaccggcaa gaagaucaac     2580

aucgacgagg ugagcaccaa cuaccagauc gaccacaucc ucccccaguc cuucaucaag     2640

gacgacagcu ucgacaaccg cgugcugacg gcgacgcccc ugaacgccga gaaguccgac     2700

gacguccccu acaagcgguu cgccaacaac uacguguccg accucaagau gaccgugggg     2760

gagaugugga agcacuggca gaaggccggc aucaucaaca agcacaagcu gggcaaccug     2820

cuccuggacc cggaccgccu gagcaaguuc cagaaguccg gguucaucaa ccggcagcuc     2880

gucgagacca gccagaucgu gaagcuggug uccgucaucc ugcagaacaa guaccccgac     2940

gcggagauca ucaccgugaa ggccggcgac aacagcgccc uccgccagcg ccuggaccug     3000

uacaaguccc gggacgugaa cgacuaccac cacgccaucg acgccuaccu cagcaucguc     3060

ugcgggaacu uccuguacca gguguacccc aaguaccgcc cguacuucgu guacggccag     3120

uucaagaagu ucucccagga ccccgaccug cagaaggacc ucaucaagca cuucaagggg     3180

uucaccuuca uguggccccu gcugcagaag gacaacagcg agcggaaggc gcucgaggag     3240

aucaaggaga acaacuccga ccgcaucguc uucuacaagc accccgacau cuucgacaag     3300

cugcggaagg ccuacaacua caaguacaug cuggugagcc gcgagaccuc caccgagagc     3360

uccagcaugu ucaacaugac gaucuacccg cggggcgagc gcgaccggaa gaagacccgc     3420

aagcucaucc ccaagggcaa cgggcuggac cccaagaucu acggcgggua cuccggcaac     3480

accgacgccu acauggugau cgucaagauc gagaaggcca aggagagcau cuacaaggug     3540

aucggcaucc ccaugcgggc ccuggcgggg cucaagcagg ccaagaacca gggcaacuac     3600

aaggaggagc ugcacaaggu ggucgagccg cagaucaugc uggacaagaa cgggaagccc     3660

aagcgcggcg uggagggguu ccgggugguc aaggaccacg ugcccuucaa gcaggucgug     3720

cucgacggcg acaagaaguu caugcuguac agcgccaagu acgagaccaa cgccaagcag     3780

cugacgcucu cccaggagac caugcgcauc gugaccgaca accugaagaa guccgaggac     3840

caggacaagc ugcucgugaa ggccuacgac gagauccugc agaaggucga ccaguaccug     3900

ccccucuucg acgugaacaa guuccggaac agccugcacc ugggccgcgc gaaguuccuc     3960

gaccuggccg ugaacgacaa gaagaucacc cugagcaaca uccucaacgg gcugcacgac     4020

aaccuggucg ugccgaaccu caagaacauc ggcaucaaga cgcccuucgg gcugaugcag     4080

ucccccagcg gcaucacccu guccagcaac gccauccuca ucuuccaguc ccccaccggg     4140

cuguucgaga agcgggugcg caucgccgac cuguga                               4176


<210>  2089
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2089
auggagaaca agcaguacua caucggccug gacgugggga ccaacagcgu cggcugggcc       60

gugaccgaca cguccuacaa ccuccugcgc gccaagggga aggacaugug gggcgcgcgg      120

cuguucgacg aggccaagac cgccguggag cgccggacca agcgcaccag ccggcgccgg      180

uccgagcgcg agaaggcccg gaaggccaug cucaaggagc uguucgcgga cgagaucaac      240

aaggucgacc ccaacuucuu cauccgccug gaggagagca aguucuuccu cgacgaccgg      300

uccgagaaca accgccagcg guacacgcug uucaacgacg ccaaguucac cgacaaggac      360

uacuacgaga aguacaagac caucuuccac cuccgcagcg cccucaucaa cuccgacgag      420

aaguucgacg ugcggcuggu guuccuggcc auccucaacc uguucagcca ccgcgggcac      480

uuccugaacg cgucccucaa gggcgacggc gagauccagg gggucgacau cuucuacaac      540

gaccuguacg aggugugcga ggaguucgag aucgaccugc cgcagaucag caacaucgac      600

aacuucgaga agauccucuc ccagaagggc aagagccgga ccaagauccu ggaggagcug      660

uccgaggagc ucagcaucuc caagaaggag aagaucaagu acagccugcu gaagcucauc      720

ugcgggauga agaucaaggu ggucgacauc uacgccgacg aggacaucca ggaggacgac      780

aagaagaagg accugggcuu ccgcgagucc gacuacgaga aguccucccu ggaggugaag      840

gagaucaucg gggacgacca cuucgagcuc aucgagcggg ccaaggccgu gcacgacauc      900

ggccugcugg ccaacaucau cggcaacagc aaguaccucu gcgaggcgcg cgucgaggcc      960

uacgacaacc accacaagga ccugcagaag aucaaggagc ugcucaagaa guacgacauc     1020

aaggccuaca cggacauguu ccggaagaug accgacaaga acuacagcgc cuacgugggg     1080

uccgugaaca gcaacaucgc caaggagcgc cgcuccgucg acaaccggaa gaucgaggac     1140

cuguacgagu acaucaagaa gaucgcgcug aagaacauca gcgacgacaa cgaggacaag     1200

aucgagaucc ucaagaagau cgagaccggc gaguuccuga agaagcagcu gaccgccucc     1260

aacgggguga uccccaacca gcuccagagc cgcgagcugc gggccauccu gaagaaggcc     1320

gagaacuacc uccccuuccu gaaggagaag ggcgagaaga accugacggu guccgagaug     1380

aucauccagc uguucgaguu ccagaucccc uacuacgucg gcccgcugga caagaacccc     1440

aagaaggaca agaacgccaa cagcugggcg gugauccccu ccgggggccg cauccugccc     1500

uggaacuucg aggacaaggu ggacgucaag gggagccgga aggaguucau cgagaagaug     1560

gugcgcaagu gcaccuaccu cuccgaggag cacacccugc cgaagcagag ccugcuguac     1620

gagaaguuca uggugcucaa cgagaucaac aacaucaaga ucgacggcga gaagaucucc     1680

gucgaggcca agcagaagau cuacaacgac cuguucauga aggggaagaa ggugagccag     1740

aaggccauca agcaggagcu gaucucccuc aacaucauga gcaaggacuc cguccugagc     1800

ggcaucgaca ccgugugcaa cgccuaccug uccagcaucg ccaaguuccg gggcguguuc     1860

gacgagaacg ucaacaacca gagcacgauc gacaugaucg aggacaucau cuuccucaag     1920

accguguacu ccgacgagaa gcgcuucgug aaggaggaga ucgucgagaa guacggggac     1980

gagaucgaca aggacaagau caagcggauc cugggcuuca aguucagcaa cugggggaac     2040

aucuccaaga gcuuccugga gcucgagggc gcggacgugg ggaccggcga gauccgcucc     2100

aucauccaga gccuguggga gaccaacuuc aaccugaugg agcuccuguc cagccgguuc     2160

acguacaugg acgagcugga gaagcgcgug aagaagcucg agaagccccu guccgagugg     2220

accaucgagg accuggacga cauguaccuc agcucccccg ucaagcggau gaucuggcag     2280

agcaugaaga ucguggacga gauccagacc gugaucggcu acgcccccaa gcgcaucuuc     2340

gucgagauga cccgguccga gggggagaag gugcgcacca agagccggaa ggaccgccug     2400

aaggagcugu acuccgccau caaggaggac agcaagcagu gggugaagga gcucgacgcc     2460

aaggacgagu ccuacuuccg gagcaagaag auguaccugu acuaccugca gaagggccgc     2520

ugcauguaca gcggggaggu caucgagcuc gacaagcuga uggacgacaa ccuguacgac     2580

aucgaccaca ucuacccgcg cuccuucgug aaggacgaca gccucgacaa ccuggugcug     2640

gucaagaagg agaucaacaa ccggaagcag aacgacccca ucacgcccca gauccaggcg     2700

uccugccagg gcuucuggaa ggugcuccac gagcaggggu ucaucagcaa cgagaaguac     2760

ucccgccuga cccggaagac ccaggaguuc agcgacgagg agaagcuguc cuucaucaac     2820

cgccagaucg uggagacggg ccaggccacc aagugcaugg cccagauccu ccagaagucc     2880

augggcgagg acgucgacgu gguguucagc aaggcccggc uggucagcga guuccgccac     2940

aaguucgagc uguucaaguc ccggcucauc aacgacuucc accacgccaa cgacgcguac     3000

cugaacaucg ugguggggaa cagcuacuuc gucaaguuca cccgcaaccc cgccaacuuc     3060

aucaaggacg cccggaagaa cccggacaac cccgccuaca aguaccacau ggaccgcuuc     3120

uucgagcggg acgugaaguc caagagcgag guggccugga ucggccaguc cgaggggaac     3180

gcgggcacca ucgccaucgu caagaagacg augagcaaga acagcccccu gaucaccaag     3240

aagguggagg agggccacgg gagcaucacc aaggagacca ucgugggcgu caaggagauc     3300

aaguucgggc gcaacaaggu ggagaaggcc gacaagacgc ccaagaaggc caaccuccag     3360

gccuaccggc cgaucaagac cuccgacgag cgccugugca acauccugcg guacggcggg     3420

cgcaccagca ucagcaucuc cggcuacugc cucguggagu acgucaagaa gcggaagacc     3480

auccgcagcc uggaggcgau ccccguguac cugggccgga aggacucccu cagcgaggag     3540

aagcugcuga acuacuuccg cuacaaccuc aacgacgggg gcaaggacuc cgugagcgac     3600

auccggcugu gccugcccuu caucuccacc aacagccucg ucaagaucga cggguaccug     3660

uacuaccugg gcgggaagaa cgacgaccgc auccagcucu acaacgccua ccagcugaag     3720

augaagaagg aggaggugga guacauccgc aagaucgaga aggccguguc caugagcaag     3780

uucgacgaga ucgaccggga gaagaacccc guccugacgg aggagaagaa caucgagcuc     3840

uacaacaaga uccaggacaa guucgagaac accguguucu ccaagcgcau gagccuggug     3900

aaguacaaca agaaggaccu guccuucggc gacuuccuca agaacaagaa guccaaguuc     3960

gaggagaucg accuggagaa gcagugcaag guccuguaca acaucaucuu caaccucagc     4020

aaccugaagg agguggaccu gagcgacauc ggcgggucca agagcaccgg caagugccgg     4080

ugcaagaaga acaucaccaa cuacaaggag uucaagcuca uccagcaguc caucacgggg     4140

cuguacagcu gcgagaagga ccugaugacc aucuga                               4176


<210>  2090
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2090
augaagcgca acaacgacua cuacaucggc cuggacaugg ggaccaacag cgugggcugg       60

gccaccacga acccccaggg gcggcuccug cgcuucaaca agaaggacau guggggcucc      120

cggcuguucg aggaggccaa gaccgcggcc aagacccgca ccuuccggac gagccgccgg      180

cgcuacgacc ggguccgcca gcggaucgag uuccuccagg acaucuucgc cccgaucauc      240

gccaagaagg acuucgaguu cuucaugcgc cugaaggagu ccaaguucca ccuggaggac      300

aagaagaccg acgggaaguu cgcccucuuc aacgacgcgg acuucaccga cguggaguac      360

uucgaccggu accccaccau cuaccaccuc cgcgccgccc ugaucaagcc caaccacauc      420

acggaccccc ggcugcugua ccuggccauc caccacauca ucaagaaccg cggccacuuc      480

cucuucgcgg gcaccgacau ggagagcauc cgguccuucc agaucgugua cacccagcug      540

agcgagcacc ugaaguccau caaccucgac uucgagcugc cggucgacgc ccaggacaag      600

uucgccgcca uccugcucaa cgagaagcug aacaagcgcg acaagaagaa gcagcugaag      660

gagcucugcc cccacaagga caagcggcug gaguacgccu ucaacgcgau caucgggggc      720

aagaccaagc ugaaggacau cuucggggug gaggaguacg acgagggcac gaagaaggcc      780

guggacuucg agaaggccga cuacgacgag gacaaggacg agaucgagag cgucaucaag      840

gagaacaucc acgugcucaa cgcccugaag gccguguacg acuggaccau ccugaccaac      900

auccucgggg acuacgcgua caucuccgag agccaggucg cccuguacga ggcccacaag      960

aaggaccugc gcauccucaa gucccugauc aagaaguacg ccaagagcca guacgccgac     1020

gcguuccggc acgagaacgu gaagggcaac uacgccaacu acgugggcuc caccaacuac     1080

gacaacaagc agcccgucau cgacaacccc agcgugacgc aggaggacau caacaaguac     1140

uucgccgaca uccugcucuc caucaccccg gacgagagcg accgcgaguc cuaccaguac     1200

aucaccgagc gccugcacaa gggggaggcc cugcccaagc uccggaccaa ggccaacagc     1260

gugcugcccc accagaucca caagaucgag cuggagaaga uccucgcgaa cgccgccaag     1320

aacuuccccu uccugcagga gaaggacucc agcggcuaca cggccgcgca gaagaucguc     1380

aagaucauga uguuccgcau cgaguacuac guggggccgc ugaacaccua ccaccacgug     1440

gaccagggcg gccacgccug gaugguccgg aaggccgagg ggaagaucac ccccuggaac     1500

uucgacgaga agguggacaa ggaggccagc gccgagaagu ucauccgcaa caugaccaac     1560

aagugcacgu accucaucgg caaggacgug auccccaagc agucccugcu guacagccgg     1620

uacaaggucc ucaacgagcu gaacaaccug aaggugaacg gggcgcccau cuccguggcc     1680

cucaagcaga agaucuacga ggagcuguuc aagaagcagg ucaacgugac cuucaaccag     1740

cugaagaccu uccugaagac ccagggcgag gacgccgacg agaucauccu cagcgggguc     1800

gacgaggccg cccacaaguu cacgaacaac cugcagaccu accacaaguu ccgcgcgauc     1860

cugcgggaca agaucgaguu cgagccgcag cgcuccaugg uggaggacau cauccggugg     1920

aagugccuca ugggcgacga caucaccaug uggaagcaca agaucgagaa ggaguacggc     1980

gaccagcuga gcgaggagca gcugaagaag aucacccgcc ucaacuucaa cggguggucc     2040

cggcugagcc aggaguuccu gacggacgug cccggcgucg acaucgaguc cggggaggug     2100

uacagcggca ucauggacgc ccucuggcgc accaacgaga acaucaugca gcugcugucc     2160

agccgguuca ccuucgccgc cgaggugaag aagcagaacc aggggaccgg ccgcaucgag     2220

aagaucacgu acgacgaggu caugaaggac accuaccucu cccccgccgu gaagcggagc     2280

guguggcagg cgaucacccu ggucgaggag auccgcaaga uccggaaggc cgaccccaag     2340

cgcaucuucg uggagaugac ccggcagccg gacgccaaga aggagcgcaa gcugucccgc     2400

cggacccagc uccugaacgc cuaccgcgcg aucggcaacg acaucgccga guacgagaag     2460

gagcacguga gcacgcugau cacccagcuc gaguccaaga aggacgagga ccugcggcgc     2520

aagaagcugu accucuacuu cacccagaug gggcggugca uguacaccgg cgagcgcauc     2580

gaccugagcu cccugcuccg ggacacgaac agcaacaucu acgacaucga ccacaucuac     2640

ccccgcagcc ugaccaagga cgacucccug gacaacaccg uccucugcaa gaaggagauc     2700

aaccugaaga aggggaacaa guacauccac cggguguuca ccgacaagga gcugaagaag     2760

caguacggcu acuggaaggc ccucaaggag aagaagcuga ucagcgagcg caaguucucc     2820

cggcugaugc gcacgaccga ccucaccgcc gacgagcugg ccggguucau cagccggcag     2880

cugguggaga ccucccagau ggucaaggcg acggccgaca uccucgagaa gaucuacccc     2940

aacgugacca ucguguacgu caaggcccgc accgugagcg acuuccggca cgacuacgag     3000

cugcccaagg ugcgcgcccu gaacgacugc caccacgccc acgacgcgua ccucaacauc     3060

gucgugggca acguguacua cgagaaguuc accaaguccc cguuccgguu caucgccaac     3120

cagaagggca agggggacau caaguacaac cuguaccgcc uguucgacaa ggacgucaag     3180

gugggcgacc ggaucaucug gaacgccaag gaggaccucg cccgcgugaa ggccacgcug     3240

gagcgggaca ccgucaacgu ggugcgcaug cccgucgagc agcgcgggca guucuucgac     3300

gcgaccaucc agaagaaggg caccacgaag aacaugcugg ugcucccccu gaagggcagc     3360

gacgagcggc ugcaggacac cuccaaguac ggguacuacg ugcgccccag cggcaugucc     3420

uucauggucg ugcagcacga ggugaagggc aagagccggg ucaccaucga gccggcccuc     3480

cugguguaca agaacaagcu gaaguccgag gacgacuacg ccaaguacug cgaggacgug     3540

cucaagcugc agaagcccaa gguccuguuc aagaacauca aguucgggag caagauccuc     3600

uucgacgggu accccuuccu gcugaagggc cgcaccgaga accggaucau cauggccgac     3660

gcgcaguccc ucuuccuggg caaccaggag cugaagaucc ucaagcgcgu gacgaagauc     3720

agcgacgaca ucgaccacaa gguggagucc cggaucaaga ucaccgacga ggaguggacc     3780

acccugugga aguccgucgu ggagcagauc gugacgggga aguuccgcaa ccggcugggc     3840

acccucaacg ggauccugca gggccgcaag aucaagcuga gcaaggacga ggaggcccgg     3900

cucaacgagc uggacagcaa ggaccaggag accuuccuga gccagauccu ccuggagcgc     3960

uuccugcagu ucuccaccga ggagaagacg cggcuccugc ugcaggucau cagcuucucc     4020

gggaacacca ccggcgugga ccuccgccug aucggcggga gcuccggcac cgggacgauc     4080

aagaccaaca agaacaucac cgaccacagc gugauccuga ucaacgaguc cccgaccggc     4140

cucuucacca accggacggu ccugaaccag cccuga                               4176


<210>  2091
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2091
augaagcgca acaacgacua cuacaucggc cuggacaugg ggaccaacag cgugggcugg       60

gccaccacga acccccaggg gcggcuccug cgcuucaaca agaaggacau guggggcucc      120

cggcuguucg aggaggccaa gaccgcggcc aagacccgca ccuuccggac gagccgccgg      180

cgcuacgacc ggguccgcca gcggaucgag uuccuccagg acaucuucgc cccgaucauc      240

gccaagaagg acuucgaguu cuucaugcgc cugaaggagu ccaaguucca ccuggaggac      300

aagaagaccg acgggaaguu cgcccucuuc aacgacgcgg acuucaccga cguggaguac      360

uucgaccggu accccaccau cuaccaccuc cgcgccgccc ugaucaagcc caaccacauc      420

acggaccccc ggcugcugua ccuggccauc caccacauca ucaagaaccg cggccacuuc      480

cucuucgcgg gcaccgacau ggagagcauc cgguccuucc agaucgugua cacccagcug      540

agcgagcacc ugaaguccau caaccucgac uucgagcugc cggucgacgc ccaggacaag      600

uucgccgcca uccugcucaa cgagaagcug aacaagcgcg acaagaagaa gcagcugaag      660

gagcucugcc cccacaagga caagcggcug gaguacgccu ucaacgcgau caucgggggc      720

aagaccaagc ugaaggacau cuucggggug gaggaguacg acgagggcac gaagaaggcc      780

guggacuucg agaaggccga cuacgacgag gacaaggacg agaucgagag cgucaucaag      840

gagaacaucc acgugcucaa cgcccugaag gccguguacg acuggaccau ccugaccaac      900

auccucgggg acuacgcgua caucuccgag agccaggucg cccuguacga ggcccacaag      960

aaggaccugc gcauccucaa gucccugauc aagaaguacg ccaagagcca guacgccgac     1020

gcguuccggc acgagaacgu gaagggcaac uacgccaacu acgugggcuc caccaacuac     1080

gacaacaagc agcccgucau cgacaacccc agcgugacgc aggaggacau caacaaguac     1140

uucgccgaca uccugcucuc caucaccccg gacgagagcg accgcgaguc cuaccaguac     1200

aucaccgagc gccugcacaa gggggaggcc cugcccaagc uccggaccaa ggccaacagc     1260

gugcugcccc accagaucca caagaucgag cuggagaaga uccucgcgaa cgccgccaag     1320

aacuuccccu uccugcagga gaaggacucc agcggcuaca cggccgcgca gaagaucguc     1380

aagaucauga uguuccgcau cgaguacuac guggggccgc ugaacaccua ccaccacgug     1440

gaccagggcg gccacgccug gaugguccgg aaggccgagg ggaagaucac ccccuggaac     1500

uucgacgaga agguggacaa ggaggccagc gccgagaagu ucauccgcaa caugaccaac     1560

aagugcacgu accucaucgg caaggacgug auccccaagc agucccugcu guacagccgg     1620

uacaaggucc ucaacgagcu gaacaaccug aaggugaacg gggugcccau cuccgucgcg     1680

cucaagcaga agaucuacga ggagcuguuc aagaagcagg ucaacgugac cuucaaccag     1740

cugaagaccu uccugaagac ccagggcgag gacgccgacg agaucauccu cagcggggug     1800

gacgaggccg cccacaaguu cacgaacaac cugcagaccu accacaaguu ccgcgccauc     1860

cugcgggaca agaucgaguu cgagccgcag cgcuccaugg uggaggacau cauccggugg     1920

aagugccuca ugggcgacga caucaccaug uggaagcaca agaucgagaa ggaguacggc     1980

gaccagcuga gcgaggagca gcugaagaag aucacccgcc ucaacuucaa cggguggucc     2040

cggcugagcc aggaguuccu gacggacguc cccggcgugg acaucgaguc cggggaggug     2100

uacagcggca ucauggacgc gcucuggcgc accaacgaga acaucaugca gcugcugucc     2160

agccgguuca ccuucgccgc cgaggucaag aagcagaacc aggggaccgg ccgcaucgag     2220

aagaucacgu acgacgaggu gaugaaggac accuaccucu cccccgccgu gaagcggagc     2280

gucuggcagg ccaucacccu gguggaggag auccgcaaga uccggaaggc ggaccccaag     2340

cgcaucuucg uggagaugac ccggcagccg gacgccaaga aggagcgcaa gcugucccgc     2400

cggacccagc uccugaacgc cuaccgcgcc aucggcaacg acaucgcgga guacgagaag     2460

gagcacguca gcacgcugau cacccagcuc gaguccaaga aggacgagga ccugcggcgc     2520

aagaagcugu accucuacuu cacccagaug gggcggugca uguacaccgg cgagcgcauc     2580

gaccugagcu cccugcuccg ggacacgaac agcaacaucu acgacaucga ccacaucuac     2640

ccccgcagcc ugaccaagga cgacucccug gacaacaccg ugcucugcaa gaaggagauc     2700

aaccugaaga aggggaacaa guacauccac cggguguuca ccgacaagga gcugaagaag     2760

caguacggcu acuggaaggc ccucaaggag aagaagcuga ucagcgagcg caaguucucc     2820

cggcugaugc gcacgaccga ccucaccgcc gacgagcugg ccggguucau cagccggcag     2880

cuggucgaga ccucccagau ggugaaggcc acggcggaca uccucgagaa gaucuacccc     2940

aacgugacca ucgucuacgu gaaggcccgc accgugagcg acuuccggca cgacuacgag     3000

cugcccaagg uccgcgcccu gaacgacugc caccacgccc acgacgccua ccucaacauc     3060

guggugggca acgucuacua cgagaaguuc accaaguccc cguuccgguu caucgcgaac     3120

cagaagggca agggggacau caaguacaac cuguaccgcc uguucgacaa ggacgugaag     3180

gugggcgacc ggaucaucug gaacgccaag gaggaccucg cccgcgucaa ggccacgcug     3240

gagcgggaca ccgugaacgu gguccgcaug cccguggagc agcgcgggca guucuucgac     3300

gccaccaucc agaagaaggg caccacgaag aacaugcugg ugcucccccu gaagggcagc     3360

gacgagcggc ugcaggacac cuccaaguac ggguacuacg uccgccccag cggcaugucc     3420

uucauggugg ugcagcacga ggucaagggc aagagccggg ugaccaucga gccggcgcuc     3480

cugguguaca agaacaagcu gaaguccgag gacgacuacg ccaaguacug cgaggacguc     3540

cucaagcugc agaagcccaa ggugcuguuc aagaacauca aguucgggag caagauccuc     3600

uucgacgggu accccuuccu gcugaagggc cgcaccgaga accggaucau cauggccgac     3660

gcccaguccc ucuuccuggg caaccaggag cugaagaucc ucaagcgcgu gacgaagauc     3720

agcgacgaca ucgaccacaa ggucgagucc cggaucaaga ucaccgacga ggaguggacc     3780

acccugugga aguccguggu ggagcagauc gucacgggga aguuccgcaa ccggcugggc     3840

acccucaacg ggauccugca gggccgcaag aucaagcuga gcaaggacga ggaggcgcgg     3900

cucaacgagc uggacagcaa ggaccaggag accuuccuga gccagauccu ccuggagcgc     3960

uuccugcagu ucuccaccga ggagaagacg cggcuccugc uccaggugau cagcuucucc     4020

gggaacacca ccggcgugga ccugcgccug aucggcggga gcuccggcac cgggacgauc     4080

aagaccaaca agaacaucac cgaccacagc gucauccuga ucaacgaguc cccgaccggc     4140

cucuucacca accggacggu gcugaaccag cccuga                               4176


<210>  2092
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2092
augaagucca uccugggccu cgaccugggg accaccagca ucggcugggc ccuggugaag       60

gagagcgaga acgacaacga gaaguccgag aucgucaagc ucggggugcg ccuggugccc      120

cugacgaccg acgagcggac cgacuucgag aagggcaaga gcaucaccac gaacgccgac      180

cgcaccucca agcggagcau gcgccggaac cuccagcgcu acaagcugcg gcgcgaggcg      240

cuggucuccg agcucaagaa ggagcacugg aucgacgagg gguaccgguu cagcgaggac      300

ggcccgggcu ccaccuucag cacccugcgc cugcgggcca aggccgccga ggaggagauc      360

ucccuccagg accuggccaa ggugcugcuc cagaucaaca agaagcgcgg guacaagagc      420

ucccggaagc ugaacagcgc ggaggacggc caggccaucg acgggauguc cguggccaag      480

guccuguacg accgcggccu cacgcccggg gaguacgugu accagcggau gcuggacggc      540

caguacgcca uccccgaguu cuacaagagc gaccugcagg cggaguucga caagaucugg      600

aaccuccaga aguccuacua ccccgagcug accgccgagc uguacgacaa gcucaccgac      660

aagaacaaga gccagaccug ggccaucugc cgcgacgagc ugggcgugca gggguucaag      720

uccagcuuca cgggcaagga gcggcugaag gaggccuacu ucaaccgcca gcaggccagc      780

aagcagaagg ucgaccucga ggugcugacc ugcgugcugc aggacaucaa cgggcagaag      840

gcgucccuca gcggccugcu guccgccauc agcgaccggu ccaaggagcu cuacuucaac      900

cgcaugaccg ucggccagau gcuggugcgg cagauccagg aggacccgaa ccgcagccug      960

cgcaacaccg uguucuaccg gcaggacuac aucgacgagu ucgagaagau cugguccguc     1020

cagagccgcu uccaccccga gcucacgcag gagcggaaga agaagcugaa ggacaucauc     1080

aucuucuacc agcgcccccu gaagucccag aaggggcuca ucagccugug cgaguucgag     1140

ucccgggagg uggacaucag cguggacggc gucaucaaga agaagaagau cgggccccgc     1200

gugugcccgc gguccagccc ccuguuccag gaguucaagg uguaccagac cgucaacaac     1260

auccucguga acggcgagcc ccuggacgac aacgaccgcg agaagcuggu gggggagcuc     1320

gccuuccggg agaagaugaa gggccaggac guccugaagc ugcucuacaa gaccuccaag     1380

ggcaugcgca ucaacuucga ggagguggag gggaaccgga ccauggccac gauccugaag     1440

gccugcgaga agaucgucga gggccugggg uacgacaccc gcgaccugag caagaucacc     1500

gcguccgaga aggugagccg gaucgaggag aucuucgcca aggagggcuu caaguccgac     1560

uuccuccacu ucgacagcag ccugcccucc ccggaguucg agagccaggc cuccuacagc     1620

cuguggcacc uccuguacuc cuacgagaac gacaagagcg uguccgggaa cgagagccug     1680

gucgagaaga ucaagaagcu cugcuccaug gacgaggcca gcgccaagau ccuguccggc     1740

ugcaccuucc ugagcgacua cggcucccuc agcgcgcgcg ccaugcggaa gauccugccc     1800

uacaugaagg acgggcugga guacuccgcc gccugcgcca uggcgggcua ccgccacagc     1860

aagcgguccc ucacgaagga ggagcuggac agccgcgugu acguggaccg gcuggagcuc     1920

cugcccaaga acagccugcg caaccccguc guggagaaga uccucaacca gaugaucaac     1980

guggucaacu ccguggugga caccuacggg aagccggacg agauccgcau cgagauggcc     2040

cgggagcuga agaaguccgc cagcgagcgc gagaaggccc agaaggacau cgcggccgcc     2100

agcaagguca acgaguccau cguggccacc cugaagggca gccccuucuu caucgacucc     2160

cccagccgga acgacaucau ccgcuaccgg cucugggagg agcugaagcc caacggguac     2220

aagacccugu acuccggcac guacaucccg aaggagcgcc ucuucagcaa ggacuucgac     2280

aucgagcaca ucauccccca ggcccugcug uucgacgacu ccuucgcgaa caagaccauc     2340

gagagccggc agugcaacau cgagaagucc aaccgcaccg ccauggacua cgucaagagc     2400

aaguacgacu ccuucggcgu gcaggccuac aucgaccggg ugaaggggcu caccggcgug     2460

agcaagacga agaucacccg ccugcugaug gaguccaagg acauccccga ggacuucauc     2520

aaccgggagc uccgcgacag ccaguacauc gcccggaagg cccgcgagau ccuggagucc     2580

auggucaagu ucguggugcc caccaccggg agcgucacgg cgcggcugcg cgaggacugg     2640

cagcucguga acgugaugcg ggagcugaac ugggacaagu acgagaagcu gggccucacc     2700

agcuuccgcg aggaccggga cggccgcgag aucccgguca ucgaggacug gaccaagcgg     2760

aacgaccacc gccaccacgc cauggacgcc cugaccaucg ccuucacccg ccggcaguuc     2820

auccaguacc ugaaccaccu caacgccaag aucgacacgg uggacaagac cgacaucgac     2880

cugcgcgacu acaaccugga ggacaucucc uucgacgggc uccgggacgg cgacaaguuc     2940

gggaucguga aggcgcugca gaagaaguac cuguacaagg acgacaagca gaaguaccgc     3000

uucgucccgc ccaugccccu cgacgaguuc cggaucgagg ccaagcgcca gaucgaggac     3060

gugcugguga gcuucaaggc caagaacaag gucugcacca agaacgugaa caccacgaag     3120

gugcggggcg ggcagcagcg caagauccag cugacgcccc ggggcccccu ccacgaggac     3180

accaucuacg gcaagcgccu gcaguacguc accaaggagg agaaggucgg gggcuccuuc     3240

gacgaggcca agaucgcgag cgugugcaag aaguccuacc gggaggcccu gaugcgccgg     3300

cucauggagu gcgggggcga ccccaagaag gccuucaccg ggaagaacag ccuggagaag     3360

aacccggugu ggguggacga guuccacucc ggcgaggugc cccugcgcgu ccggaccgug     3420

agcauggccu ccguguacac cguccgcaag agcgugggcc agucccucaa caucgagaag     3480

gugcuggaca agcacaucaa ggagauccug cgggcccgcc ucgaggagua cgggggcaac     3540

gcgagcaagg ccuucuccaa ccuggacgag aaccccaucu ggcugaacaa ggagaagggg     3600

aucgccguca agcgggugac caucgacagc ggcaagaacc ccaucccgcu ccgcuccaag     3660

cuggacaagg acgggcagau caucuacggc gagggccaga acccccagga gagcgacuuc     3720

gugguccccg ggggcaacca ccacgccgcc aucuaccgca cgcccuccgg gaagcugcag     3780

gacguggugg ucagcuucua cgaggcggug ucccggaaga accucggccu ggaggucauc     3840

gaccgcaacu ucaagaagga cgaggggugg gaguuccugu ucacccucaa gcagaacgag     3900

uacuucgugu ucccgaaccc cgccaccggc uucagccccg aggacuacga ccugaccgac     3960

cccgccaaca acgccaucau cagcccgaac cuguaccggg ugcagacggu cuccgugggc     4020

gacuaccgcu uccggcacca ccucgagacc accguggagg acaacgagaa gcugcgcgac     4080

aucgccugga agcggaucgg gcccagcggc cuggucgggg cggugaaggu gcgcaucgac     4140

aaccucggcc ggauccuggc cgucggcgag uacgacuga                            4179


<210>  2093
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2093
auggagaaca agaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucaccg acgacuacaa ggugcccagc aagaagauga agguguucgg gaacacggac      120

aagcacuuca ucaagaagaa ccucaucggc gcccugcugu ucgacgaggg ggcgaccgcc      180

gaggaccgcc ggcucaagcg caccgcccgg cgccgguaca cccgccggaa gaaccgccug      240

cgguaccugc aggagaucuu cuccgaggag aucagcaagc ucgacuccag cuucuuccac      300

cgccuggacg acuccuuccu ggucccgaag gacaagcggg gcagcaagua ccccaucuuc      360

gccacgcucg aggaggagaa ggaguaccac aagaaguucc ccaccaucua ccaccuccgc      420

aagcaccugg ccgacuccaa ggagaagacc gaccugcggc ugaucuaccu ggcgcucgcc      480

cacaugauca aguaccgcgg ccacuuccug uacgaggaga gcuucgacau caagaacaac      540

gacauccaga agaucuucaa cgaguucauc uccaucuacg acaacaccuu cgaggggagc      600

ucccugagcg gccagaacgc ccagguggag gccaucuuca cggacaagau cuccaagagc      660

gcgaagcggg agcgcgugcu caagcuguuc uccgacgaga aguccaccag ccuguucagc      720

gaguuccuca agcugaucgu cgggaaccag gccgacuuca agaagcacuu cgaccuggag      780

gagaaggcgc cccuccaguu cagcaaggac accuacgacg aggaccugga gaaccugcuc      840

ggccagaucg gggacggcuu caccgaccug uuccuggugg ccaagaagcu cuacgacgcc      900

auccugcugu ccggcauccu cacggugacc gacccgagca ccaaggcgcc ccuguccgcc      960

agcaugaucg agcgguacga gucccaccag aaggaccugg ccgcccucaa gcaguucauc     1020

aagaacaacc ugcccaagcg cuacaacgag gucuucagcg accaguccaa ggacggguac     1080

gccggcuaca ucgacgggaa gaccacgcag gaggcguucu acaaguacau caagaaccug     1140

cucagcaagu ucgagggcgc cgacuacuuc cuggacaaga ucgagcggga ggacuuccug     1200

cgcaagcagc ggaccuucga caacggcucc aucccccacc agauccaccu ccaggagaug     1260

aacgccaucc ugcgccgcca gggggagcac uacccguucc ugaaggagaa ccgggagaag     1320

aucgagaaga uccucaccuu ccgcaucccc uacuacgugg gcccccuggc ccgggggaac     1380

cgcgacuucg ccuggcugac ccggaacagc gaccaggcga uccgccccug gaacuucgag     1440

gagaucgugg acaaggccuc cagcgccgag gaguucauca acaagaugac gaacuacgac     1500

cucuaccugc cggaggagaa gguccugccc aagcacuccc ugcucuacga gaccuucgcc     1560

guguacaacg agcugaccaa ggugaaguuc aucgccgagg gccugcggga cuaccaguuc     1620

cucgacagcg ggcagaagaa gcagaucguc aaccagcugu ucaaggagaa gcgcaaggug     1680

accgagaagg acaucaucca cuaccugcac aacguggacg gcuacgacgg caucgagcuc     1740

aaggggaucg agaagcaguu caacgcgucc cugagcacgu accacgaccu gcucaagauc     1800

aucaaggaca aggaguucau ggacgacccc aagaacgagg agauccugga gaacaucguc     1860

cacacccuga ccaucuucga ggaccgggag augaucaagc agcgccucgc ccaguacgac     1920

uccaucuucg acgagaaggu gaucaaggcc cugacccggc gccacuacac gggcuggggg     1980

aagcugagcg ccaagcucau caacggcauc ugcgacaagc agaccgggga caccauccug     2040

gacuaccuga ucgacgacgg caagaucaac cggaacuuca ugcagcucau caacgacgac     2100

ggccuguccu ucaaggagau cauccagaag gcccaggugg ucgggaagac cgacgacgug     2160

aagcaggucg ugcaggagcu gcccggcagc ccggccauca agaaggggau ccuccagagc     2220

aucaagaucg uggacgagcu ggucaaggug augggcuacg cccccgaguc caucgugauc     2280

gagauggccc gcgagaacca gacgaccgcc cgggggaaga agaacagcca gcagcgcuac     2340

aagcggaucg aggacucccu gaagaaccuc gcgcccggcc uggacagcaa cauccugaag     2400

gagaacccca ccgacaacau ccagcuccag aacgaccgcc uguuccugua cuaccuccag     2460

aacggcaagg acauguacac cgggaagccg cuggacaucg accagcuguc cagcuacgac     2520

aucgaccaca ucauccccca ggccuucauc aaggacgacu ccaucgacaa ccggguccuc     2580

acgagcucca aggacaaccg cggcaagagc gacaacgugc ccucccugga ggugguccag     2640

aagcgcaagg ccuucuggca gcagcugcuc gacagcaagc ugaucuccga gcggaaguuc     2700

aacaaccuga ccaaggccga gcgcgggggc cucgacgagc gggacaaggu gggcuucauc     2760

cgccggcagc ugguggagac ccgccagauc accaagcacg ucgcccagau ccuggacgcg     2820

agcuucaaca ccgaggugaa cgagaagaac cagaagaucc ggacggugaa gaucaucacc     2880

cucaagucca accuggucag caacuuccgc aaggaguucg agcuguacaa ggugcgggag     2940

aucaacgacu accaccacgc ccacgacgcc uaccucaacg ccguggucgc caaggcgauc     3000

cugaagaagu accccaagcu ggagccggag uucguguacg gggacuacca gaaguacgac     3060

cucaagcgcu acaucucccg guucaagccc agcaaggaga ucgagaaggc caccgagaag     3120

uacuucuucu acagcaaccu gcugaacuuc uucaaggagg aggugcacua cgccgacggc     3180

aucaucguca agcgcgagaa caucgaguac uccaaggaca ccggggagau cgccuggaac     3240

aaggagaagg acuucgcgac gaucaagaag gugcucagcu acccccaggu gaacaucguc     3300

aagaagaccg agauccagac ccacggccug gaccggggga agcccaaggg ccuguucaac     3360

uccaacccga gccccaagcc cuccgaggac agcaaggaga accucgugcc gaucaagcag     3420

ggccuggacc cccgcaagua cgggggcuac gccgggaucu ccaacagcua cgccgugcug     3480

gucaaggcca ucguggagaa gggcgccaag aagcagcaga agaccauccu cgaguuccag     3540

gggaucucca uccuggacaa gaucaacuuc gagaacaaca aggagaacua ccugcucaag     3600

aagcgguaca ucgagauccu gagcacgauc acccugccca aguacucccu cuucgaguuc     3660

cccgacggca cccgccggcg ccuggcgagc auccugucca ccaacaacaa gcggggcgag     3720

auccacaagg ggaacgagcu cgugcugccg ggcaaguaca cgacccugcu cuaccacgcc     3780

aagaacauca acaagaagcu ggagcccgag caccuggagu acgucgagaa gcaccgcaac     3840

gacuucgcca agcuccugga gugcgugcug aacuucaacg acaaguacgu gggggcccuc     3900

aagaacggcg agcggauccg ccaggccuuc accgacuggg agaccgucga caucgagaag     3960

cugugcuuca gcuucaucgg gcccgagaac uccaagaacg cgggccuguu cgagcucacg     4020

agccagggcu ccgccagcga cuucgaguuc cuggggguga agaucccccg cuaccgggac     4080

uacgccccgu ccagccugcu caaggccacc cugauccacc agagcaucac cggccuguac     4140

gagacccgca ucgaccucuc caagcugggg gaggacuga                            4179


<210>  2094
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2094
augaacaaca acaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucaccg acgacuacaa ggugcccagc aagaagauga aggugcucgg gaacacggac      120

aagcacuuca ucaagaagaa ccugaucggc gcccuccucu ucgacgaggg ggcgaccgcc      180

gaggaccgcc gguucaagcg caccgcccgg cgccgguaca cccgccggaa gaaccgccug      240

cgguaccugc aggagaucuu cuccgaggag augagcaagg ucgacuccag cuucuuccac      300

cgccucgacg acuccuuccu ggugccggag gacaagcggg gcagcaagua ccccaucuuc      360

gccacgcugg ccgaggagaa ggaguaccac aagaaguucc ccaccaucua ccaccuccgc      420

aagcaccugg cggacuccaa ggagaagacc gaccugcggc ucaucuaccu ggcccuggcc      480

cacaugauca aguaccgcgg ccacuuccuc uacgaggaga gcuucgacau caagaacaac      540

gacauccaga agaucuucuc cgaguucauc agcaucuacg acaacaccuu cgaggggucc      600

agccuguccg gccagaacgc ccagguggag gcgaucuuca cggacaagau cagcaagucc      660

gccaagcggg agcgcauccu gaagcuguuc gccuacgaga aguccaccga ccuguucagc      720

gaguuccuga agcucaucgu cgggaaccag gccgacuuca agaagcacuu cgaccuggag      780

gagaaggcgc cccugcaguu cagcaaggac accuacgacg aggaccucga gaaccugcug      840

ggccagaucg gggacgacuu cgccgaccuc uuccuggugg ccaagaagcu guacgacgcc      900

auccuccuga gcggcauccu gaccgugacg gacuccagca ccaaggcccc gcucuccgcc      960

agcaugaucg agcgguacga gaaccaccag aaggaccugg cggcccugaa gcaguucauc     1020

cagaacaacc uccaggagaa guacgacgag gucuucuccg accagagcaa ggacggcuac     1080

gcccgcuaca ucaacgggaa gaccacccag gaggccuucu acaaguacau caagaaccug     1140

cuguccaagu ucgagggcag cgacuacuuc cucgacaaga ucgagcggga ggacuuccug     1200

cgcaagcagc ggacguucga caacgggucc aucccccacc agauccaccu ccaggagaug     1260

aacgccauca uccgccgcca gggcgagcac uaccccuucc ucaaggagua caaggagaag     1320

aucgagacca uccugaccuu ccggaucccc uacuacgugg gcccgcuggc gcgcgggaac     1380

cggaacuucg ccuggcucac ccgcaacagc gaccaggcca uccggcccug gaacuucgag     1440

gagaucgugg accaggccuc cagcgccgag gaguucauca acaagaugac gaacuacgac     1500

cuguaccugc ccgaggagaa gguccucccc aagcacuccc ugcuguacga gaccuucgcg     1560

guguacaacg agcugaccaa ggugaaguuc aucagcgagg gccuccgcga cuaccaguuc     1620

cuggacuccg ggcagaagaa gcagaucguc aaccagcugu ucaaggagaa gcggaaggug     1680

accgagaagg acaucaucca guaccuccac aacguggacg gcuacgacgg gaucgagcug     1740

aagggcaucg agaagcaguu caacgccagc cuguccacgu accacgaccu ccugaagauc     1800

aucaaggaca aggaguucau ggacgacccg aagaacgagg agauccugga gaacaucguc     1860

cacacccuca ccaucuucga ggaccgcgag augaucaagc agcggcuggc ccaguacgcc     1920

agcaucuucg acaagaaggu gaucaaggcg cugacccgcc ggcacuacac gggcuggggg     1980

aagcucuccg ccaagcugau caacggcauc ugcgacaaga agaccgggaa gaccauccug     2040

gacuaccuca ucgacgacgg cuacagcaac cgcaacuuca ugcagcugau caacgacgac     2100

gggcugagcu ucaaggacau cauccagaag gcccaggugg ucggcaagac caacgacgug     2160

aagcaggucg ugcaggagcu ccccggcucc cccgccauca agaaggggau ccugcagagc     2220

aucaagcugg uggacgagcu cgucaaggug augggccacg cccccgaguc caucgugauc     2280

gagaucgcgc gggagaacca gacgaccgcc cgcgggaaga agaacagcca gcagcgguac     2340

aagcgcaucg aggacgcccu gaagaaccug gccccgggcc ucgacuccaa cauccugaag     2400

gagcacccca ccgacaacau ccagcugcag aacgaccggc uguuccugua cuaccugcag     2460

aacgggaagg acauguacac cggcgaggcc cucgacauca accagcugag cuccuacgac     2520

aucgaccaca ucguccccca ggcguucauc aaggacgaca gccuggacaa ccgcgugcuc     2580

acguccagca aggacaaccg gggcaagucc gacaacgugc ccagccugga ggucgugcag     2640

aagcgcaagg ccuucuggca gcagcugcuc gacuccaagc ugaucagcga gcacaaguuc     2700

aacaaccuga ccaaggccga gcgcgggggc cucgacgagc gggacaaggu gggguucauc     2760

cgccggcagc uggucgagac ccgccagauc accaagcacg uggcccagau ccuggacgcc     2820

cgguucaaca ccgaggugaa cgagaaggac aagaagaacc gcacggucaa gaucaucacc     2880

cucaagucca accuggugag caacuuccgg aaggaguuca agcuguacaa ggugcgcgag     2940

aucaacgacu accaccacgc gcacgacgcc uaccucaacg ccgucguggc caaggcgauc     3000

cugaagaagu acccgaagcu ggagcccggc uucguguacg gcgacuacca gaaguacgac     3060

aucaagcggu acaucucccg cagcaaggac cccaaggagg ucgagaaggc caccgagaag     3120

uacuucuucu acagcaaccu ccugaacuuc uucaaggagg aggugcacua cgccgacggg     3180

accaucguga agcgggagaa caucgaguac uccaaggaca cgggcgagau cgccuggaac     3240

aaggagaagg acuucgccac caucaagaag guccugagcc ucccccaggu gaacaucgug     3300

aagaagaccg agauccagac ccacgggcug gaccgcggca agccgcgggg gcuguucaac     3360

uccaacccca gccccaagcc cuccgaggac cgcaaggaga accucguccc gaucaagcag     3420

ggccuggacc cccggaagua cggcggguac gcgggcauca gcaacuccua cgccgugcug     3480

gugaaggcca ucaucgagaa gggggccaag aagcagcaga agacgguccu cgaguuccag     3540

ggcaucagca uccuggacaa gaucaacuuc gagaagaaca aggagaacua ccugcucgag     3600

aagggguaca ucaagauccu guccaccauc acccugccca aguacagccu cuucgaguuc     3660

ccggacggca cccgccggcg ccuggccucc auccugagca cgaacaacaa gcggggcgag     3720

auccacaagg ggaacgagcu cgugaucccc gagaaguaca ccacccugcu guaccacgcg     3780

aagaacauca acaagacccu cgagcccgag caccuggagu acguggagaa gcaccgcaac     3840

gacuucgcca agcugcucga guacguccug aacuucaacg acaaguacgu gggcgcccug     3900

aagaacgggg agcggauccg ccaggccuuc aucgacuggg agacggugga caucgagaag     3960

cucugcuucu ccuucaucgg cccccgcaac agcaagaacg ccgggcuguu cgagcugacc     4020

ucccagggca gcgcguccga cuucgaguuc cucggcguca agaucccgcg guaccgcgac     4080

uacaccccca gcucccugcu gaacgccacc cucauccacc agagcaucac ggggcuguac     4140

gagacccgga ucgaccugag caagcucggc gaggacuga                            4179


<210>  2095
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2095
augaagaacu accacaucgg ccuggacauc gggaccagcu ccgugggcuu cgccgcccgc       60

gacgacgaga acaaccucau ccgggucaag gggaagaacg ugaucggcgc gcgccuguuc      120

gccgagggga agaccgccca ggagcggcgc auggcccgga cgagccgccg gcgcuacaac      180

cggcgcaagu ggcggcugcg ccuccugaac gacaucuucc acgacgagau cgugcacaag      240

gacaaggacu ucuucaaccg gcugaagcag uccagcaucu cccccaagga cgaccgcaag      300

aaguacuucg gcagccuccu guucccggag cuguccgaca gcgaguucua ccacaacggc      360

aaccagacca ucuaccaccu ccggcaccgc cugaugaccu ccaacgagaa ggccgacauc      420

cgggagaucu accuggcgcu ccgccacauc gucaaguacc gggggaacuu ccuggacgac      480

acccccguga gcuccuucga ggccagcgac cugaagcucg gcuccuucau ccccacgcug      540

aacgaccugu ucgagaccau cgacgugaac uacgucuuca acagcgagaa caccaaccag      600

aucgagucca uccuccugaa caacgaccug cgcaacagcg acaaguccaa gcagcucgug      660

gaccugcugu accggaacgu gagcgacguc gacgacaagu cccucgagaa gcaguucaac      720

aaguuccgca aggacaucag caaggagauc gccaagagcu ucgugggguc caaguucaac      780

gugaacaagc ugcuggccua cgagaccgac gacggcagca agaucucccu cagcuucucc      840

gacgcgagcu ccgacgacaa gaugcuggag cuggccgaca accucgacga cgagcgcauc      900

aacauccuga acgcccugaa ggucaucuac agccgggugc gccucaacca gaucaucccc      960

aacgggaugg gccuguccga gagcaugauc gccuccuacg agcugcacaa gaagcagcuc     1020

aacgaccuga agaagcugcu caacgugcug ccguuccacg agaaguacgc cgucaaggug     1080

gcguacgccg ccuacaucgg caacauggac gcccagaagu ucacgcaggc cgagcugagc     1140

accaucgagu cccucaagga gaaggacagc aucaagaagg ugaucgacug caagaccggg     1200

cacaucacca acgagucccu gaucagcguc aucaaccaga ucguggugaa ccccuaccug     1260

gagaacuccg acuucgagcu caucgacauc gucaagaaga ucgaccagga caucaagaac     1320

ggcgaguacc ugcugaagca gcggaacaac cagaacggga acauccccca ccagcuccac     1380

cagauggaga uggaccagau caucgagaag cagagcaagu acuaccccuu ccugggcgag     1440

cugaacccga acgagaaccg ccggcacauc gcgaaguaca agaucuccga gcugguggcc     1500

uuccgcgugc ccuacuacgu cggcccccuc aucacgcccg aggagcaggc caagaccagc     1560

ggggccagcu ucgccuggau gaagcggaag gaggacggcg agaucacccc guggaacuuc     1620

gaccagaagg uggaccgcga ggcgaccgcc ucccgguuca ucaagcgccu gacggucaag     1680

gacaccuacc uguucaacga ggacgugcuc cccgacaaca gccugcugua ccagcaguuc     1740

aaggugcuca acgagcugaa cgucgugaag gugaacaaca agcggcuguc ccaggccgag     1800

aagcaggggg ccuucaacga ccucuucaag gucaccaaga ccgugacggc caagcgccug     1860

aagaacuacc uggugagcaa cuucaaccac cucgacacca ucgagaucac cggccugucc     1920

gacggggaga aguucaacaa cggccugacc agcuacgucg acuuccagaa gaucuucggc     1980

gacaagauca acaaccucga gcugauggac gacuucgagu cccugaucga guggagcacg     2040

guguucgagg accggcacau cuucgaggag aaggugcgca acgagaucaa cuggaucucc     2100

gagagcgagc ucaagcagcu ggucgcgaag cgguacuccg gguggggccg ccugagcucc     2160

aagcuccugg uggggaucaa caacgacgag ggccggaaca ucaucgagca gcuguucgag     2220

accagcgaga ccuucaugga ggccaucucc cgccccgagg ugaagaagca gaucgacgag     2280

aucaacagcg acuucaucaa gaacaccgac aucgaguccg uccucgccga cgccuacacg     2340

agcccccaga acaagaaggc gauccgggag accuacaaga ucgugaagga cauccagaag     2400

gccaugggcg ggaaggcccc ggagagcauc uccaucgagu ucacccgcaa ccccgaggac     2460

aagggcgagc ugacccgcag ccggcacucc gagaucagcc gcaaguacaa ggagcugucc     2520

aaggagauca gcaaggagcu caacgacgag cugaagucca agaagaacag cauggacauc     2580

gacaaguacu accuguacuu caugcagaug gggaaggacc ucuacgacgg caaguccauc     2640

gacaucgacg accugaacaa cuaccagauc gaccacaucg ugccccagag cuucuacaag     2700

gacgacuccu ucgacaaccg gguccugacg uacuacacca acaaccagaa gaaggggaac     2760

aacacgcccc ucaacggccu gaacaucagc aacgagaccc gcaagcugug gucccaccuc     2820

cacgaccugg ggcugaucag caagcggaag uaccucaacc ugaccaugca gggccuggac     2880

ucccucagca aguacaccaa gcacggcuuc auccgccggc agcuggugga gaccucccag     2940

gugaucaagc uggucgccaa cauccucagc gacgaguucu ccgccgacaa caccaagauc     3000

aucgagguga aggcgaagau gaacagccag augcgccaca gcuucaaccu guacaagauc     3060

cgggagcuga acgacuacca ccacgcccuc gacgccuacc ugaccacguu caucgggggc     3120

uaccuguaca accgcuaccc gaagcuccgg gggauguucg uguacggcga cuucaagaag     3180

uucgacguca acgacgccaa caacgugaag acccugaacu uccugcacga caucaccaac     3240

cccuccgaca aguucgugaa ccagaucuac gacgagcaga gcggcgagcu cauccuggac     3300

cgcaagcacg ccaucgagac caucaagaag aucuaccagu acaaguucau gcugaucacg     3360

cgggaggucu ccaccaagga cgacaagcuc agcgaccaga ccguguacuc cgcgaagcgc     3420

aucaacaacg uggccagccc cauccccguc aagaaggacu ccaacccgga gcuguacggg     3480

uuccggaccg gcaacgugga ccaccacaug gugaucguca aguaccccaa gaaggacagc     3540

uucguguaca agguggucgg gauccccauc cgcuucaugc aggagcugcg guccagcaag     3600

aaccaguccg aguacgagaa cacgcucaac cagaucgugc aggccaagcu gaaccgcaag     3660

aaguucgacu uccggguggu cguggacaac gugauguacg gccagcugau ccaggacggg     3720

ggccagaagu ucacccucgg cagcuccacc uaccgcuaca acgccaagca gcugguccug     3780

gacauggaca gccuccggau ccuguccgac gagaaguacc gcaacagccu gaccgacgac     3840

gagaagaacu ccgaguucau caacaucuac cagcaggucc ucaacguggu gaacgagaag     3900

uucgacgugu ucgacgugcg cgggacgcgg gacaagcuga ccaagggccu ggacaaguuc     3960

cucaacauca ccagcaacga caaggagauc guccugcuga agauccucuc cgggcugcac     4020

ugcaacgccg cgcgcgugaa gaucaacgag aucggcauca acaccagcuu cggggagcug     4080

cagacgaccc acggcaucgu gcucagcgag aacuccuaca ucaucuacca gagccccucc     4140

ggccuguucg agcggaaggu ccgccugaag gaccucuga                            4179


<210>  2096
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2096
auggccaaca agaccuggua ccugggccuc gacaucggga gcgccuccgu gggcugggcg       60

gccaccgaca cgggguacaa gaucauccgc aagaacaaga agcggcugug gggcguccgc      120

cuguucgagg aggccaagac cgccgaggac cggcgcgggu accggagcuc ccgccggcgc      180

cucgcccggc gcaaguggcg gcugaaccug cucgaggagc uguucaccag cgaggugaag      240

aagguggacg agaacuucuu ccugcgccuc aaggaguccc aguaccacua cgaggacaag      300

acccacaagg uccccuacgc gaucuucaac gacaaggacu acacggacaa ggacuacuac      360

aaggacuacc cgaccaucua ccaccuccgg agcaagcuga ugaccgagga gaaccccgac      420

auccgcaagg uguuccuggc cauccaccac aucaucaaga accggggcca cuuccugcug      480

cagggccagu ccuucaagga cgggaaccuc aacaaccuga ucaaggagcu gcucgagcug      540

gacauccugc acgugggcuu cgaggucacc gaggaggugg uggacaagau cgccgacauc      600

agccucgaga agaagacguc caaggacaag cugaacgaca ucaaggagcu guaccccaag      660

gagaagcagc uccuggaggu cuuccgccug aucuucgggg gcaagaccag ccucgacaag      720

cuguucgcca ucgacgagua caaggagcug gacgcggcca ucaaguccgu gagcuucaag      780

gagaagaucu acgaggaggu gcggcacgac uacgagcagg uccucuccaa cuacaucgag      840

cugcuggacc ucgccaagcu gguguacgac agcaucaucc uguccgacau caagaaggag      900

gggaagaccc ucagcgaguc caagguggag cuguucgaga agcaccgcga cgaccugcuc      960

aagcugaaga accuggucaa gaacgacagc aagcucuccg aggacaagaa gguggagcug     1020

uacgcccuga ucuucaagga ggacaaggac aagggcacca acuacgugaa cuacauccgc     1080

aagagcgagg agggcaaggg gugcaacuac gaggacuuca agaaguuccu cgucaaggag     1140

cuggccaagc uggaggagag cuccgugaag acggagauca ucaaggaccu cgagcuggag     1200

caguuccugc cccugcagcg gaccaaggac aacagcgugg ucccguacca gauccacaag     1260

gaggagcucg ugaagauccu ggacaacgcg gcccgguacc acuccuuccu gaacgagaag     1320

gacgagagcg gcuacaacgu gcgcgagaag gucauccagc uccuggaguu ccgcaucccc     1380

uacuacgugg ggccccugaa cuccagcaag aaggccaagg agggcggcuu cgccuggucc     1440

guccggaaca ccggguacga gaacaccccc gugacgccgu ggaacuacag caaggugauc     1500

gacgaguccg ccagcgcgga gaaguucauc accaaccuca ccaacaagug caccuaccug     1560

aagggcgagg acguccugcc caaguccagc cuccuguacu ccgaguucgc ccugcucaac     1620

gagcugaacg cccugaagua cgacgggaac cgcaucagcc ucgaggcccg gaacacgauc     1680

aucgagaagc uguucaagca gcagggcaag aaggugacca agaccuccau caagaaccug     1740

cucaaggccg agggguacau cgacggcaag ggcgagauca ccgggaucga caucacggug     1800

aagaacgacc ucaagagcua ccgcgacuuc aagaagaucc ugggcaacaa guucaacucc     1860

gagcacgucg agaacaucau ccuguggauc acccuguacg gggagagccg gaagcugauc     1920

aaggcgaaga ucgaguccgu guacggcgag guguacagca aggacgagau caccaagaug     1980

agccgccucg ucuacaagga cugggggcgg uucucccgca agcugcugac cgagcucgug     2040

agcaagaagc uguacaacga ggagacgggc gagugccuga acaucaucgg cgccaugcgg     2100

cagaacaaca uccucuucau ggagcugcug gccgaccgcu ucgacuacuc caagcagauc     2160

guggaguuca acaaggagcu ccaggaggac gucaccgaga ucgcccccga gauccuggac     2220

gaccuguacg ugagccccgc cgugaagcgg uccaucuggc agaccguccg caucguggag     2280

gagcucaaga agaucaucgg gugcgcgccg gccaagaucu ucguggagac cacgcggagc     2340

aaccaggaga agaagaagcc caccgacucc cgcaagaagc agcuggagcu ggccuacaag     2400

gccgucaaga aggacgugaa ggagcucgag aaggagaucg gcaccaucaa cuucgacgug     2460

cugaacgacc ggcugagcuc cgucgagccc agcaagcuca aggcgaagaa gcuguaccug     2520

uacuacaccc agcucgggcg cugcauguac uccgaggagc ccaucaacau cagcgagcug     2580

uucgacaaca acaaguacga caucgaccac aucuacccgc aguccaagau caaggacgac     2640

agcuucacga acaccgugcu ggugaagcgg gaguccaacg ccgccaagac cgaccgcuac     2700

ccccucggca gcgacaucca gacccccucc aaccggcgcu ucuggaaguu ccugaaggag     2760

aaggggcuga ucagcgacga gaaguacaac cgccucgucc ggaccgagga guucagcgac     2820

gaggagcugu ccggcuucau cgcccgccag cugguggaga cgagccaguc cgugaaggcc     2880

gucgcgagca uccucggcga gcugaacccc gagaccucca ucugcuacag caaggccgag     2940

aacgugucca gcuuccggca gaacuucggg aagaucaaga agggcgaccg cgacuccaag     3000

aacaacgacc agcugaucaa ggugcgggag aucaacgacu accaccacgc caaggacgcc     3060

uaccucaacg ucgugguggg gaacgucuac gacaccaagu ucacccgcaa cguguucaac     3120

uucaucaagg acaagaagga cggccggaag uacagccuga acaagguguu cuacgagaac     3180

gucuccgaca gcaagacggu ggccugggag auggacaaga ccauccacca gguggagaag     3240

gucaugaaca acaacaacau cauggugacc cgccggaccu ccgagcagaa gggcgggcug     3300

uucgacgcga cgguguacaa ggccaagguc gccgccaagg ccaaggacgg cguguacuac     3360

ccgcucaaga ccagcaacuc cguggucaag gacguggcga aguacggggg cuacaccagc     3420

aucgggaucg ccuacuacuc caucuucgag uacaccaucg ugaacaagaa gggcgaggag     3480

aagaucacgc gcaucauccc cauccccauc uacaucagcc agaacaucaa ggacuccacc     3540

gcccugaucg aguucggcaa gagccagauc uucugccggg ccuacgagaa gaucaaggac     3600

uucaagcugc ucuaccgcaa gcugugcauc gggagcaaga ucaacaucaa caacuaccag     3660

uacuacaucg gcgggaaguc cgacgagcgg uucuacuacg acagcgcgau ccaggucacc     3720

cuggacaaga aguccgagaa guaccucaag auccugagca aguaccagaa cuggaagaag     3780

gagaacaagg acggcgagcu gugggagaac aucaccaagg agaacaacau cgagcucuac     3840

aacaagcugg uggagaagau gaacuccgag aucuucacga agaagaaguc caacaaguac     3900

aacgagcuga acagcgagga gauccgcaac aacuucauca agaucagcgu ggaggagcag     3960

gccaaggucc uccuggagau ccugaaccuc cugaccaaca agaaguccau cuucgaccuc     4020

aagagcaucg acaucaaggc cgcccggggg aagaucuccu ucaaccugac cagccugauc     4080

caguucucca ucaucgagca gagcaucacc ggcuucuacg agaaggagau cacgaucauc     4140

ggcgacaagg ggaacgacau ggagaacaac aacuccuga                            4179


<210>  2097
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2097
augaacaaca acaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucaccg acgacuacaa ggugcccagc aagaagauga aggugcucgg gaacacggac      120

aagcacuuca ucaagaagaa ccugaucggc gcccugcugu ucgacgaggg gaccaccgcg      180

gaggaccgcc ggcugaagcg caccgcccgg cgccgguaca cgcgccggaa gaaccgccug      240

cgguaccucc aggagaucuu cuccccggag aucagcaagg ucgacuccag cuucuuccac      300

cgccuggacg acuccuuccu ggugcccgag gacaagcggg gcagcaagua ccccaucuuc      360

gccacccucg ccgaggagaa ggaguaccac aagaacuucc ccaccaucua ccaccuccgc      420

aagcagcugg ccgacuccaa ggagaaggcg gaccuccggc ugaucuaccu ggcccucgcc      480

cacaugauca aguaccgcgg ccacuuccug uacgaggaga gcuucgacau caagaacaac      540

gacauccaga agaucuucaa cgaguucauc uccaucuacg acaacaccuu cgaggggagc      600

ucccugagcg gccagaacgc ccagguggag gcgaucuuca cggacaagau cuccaagagc      660

gccaagcggg agcgcguccu caagcuguuc ccggacgaga aguccaccgg gcuguucagc      720

gaguuccuca agcugaucgu gggcaaccag gccgaguuca agaagcacuu cgaccuggag      780

gagaaggcgc cccuccaguu cuccaaggac accuacgacg acgaccugga gaaccugcuc      840

gggcagaucg gcgacggcuu cgccgagcug uucguggcgg ccaagaagcu guacgacgcc      900

auccuccuga gcgggauccu gaccgucacg gaccccagca ccaaggcgcc ccucuccgcc      960

agcaugaucg agcgguacga gaaccaccag aaggaccugg ccgcccugaa gcaguucauc     1020

cagaacaacc uccaggagaa guacgacgag guguucuccg accagagcaa ggacggcuac     1080

gccggguaca ucaacggcaa gaccacccag gaggccuucu acaaguacau caagaaccug     1140

cuguccaagu ucgagggcag cgacuacuuc cucgacaaga ucgagcgcga ggacuuccug     1200

aagaagcagc ggacguucga caacgggucc aucccgcacc agauccaccu ccaggagaug     1260

aacgccauca uccgccggca gggcgagcac uaccccuucc uccaggagaa caaggagaag     1320

aucaagaaga uccugaccuu ccgcaucccc uacuacgugg ggccccuggc gcgcggcaac     1380

ggggacuucg ccuggcucac ccggaacagc gaccaggcca uccgcccgug gaacuucgag     1440

gagaucgucg accaggccuc cagcgccgag gacuucauca acaagaugac caacuacgac     1500

cuguaccugc ccgaggagaa ggugcucccc aagcacuccc ugcuguacga gacguucgcg     1560

guguacaacg agcugaccaa ggucaaguuc aucgccgagg gccuccggga cuaccaguuc     1620

cuggacagcg gccagaagaa gcagaucgug aaccagcugu ucaaggagaa gcgcaaggug     1680

accgagaagg acaucaccca guaccuccac aacgucgacg gguacgacgg caucgagcug     1740

aaggggaucg agaagcaguu caacgccucc cugagcacgu accacgaccu ccugaagauc     1800

aucaaggaca aggccuucau ggacgacgcg gagaacgagg ccacccugga gaacaucauc     1860

cacacccuca ccaucuucga ggaccgggag augaucaagc agcgccuggc ccaguacgac     1920

ucccuguucg acgagaaggu gaucaaggcc cucauccggc gccacuacac gggcuggggg     1980

aagcugagcg ccaagcugau caacggcauc ugcgacaaga agaccggcaa gaccauccuc     2040

gacuaccuga ucgacgacgg guacuccaac cggaacuuca ugcagcugau caacgacgac     2100

ggccucagcu ucaaggacau cauccagaag gcccaggugg ucgggcgcac caacgacgug     2160

aagcagaucg ugcacgagcu gcccggcucc ccggccauca agaaggggau ccugcagagc     2220

aucaagaucg ucgacgagcu cgugaagauc augggccaca cgcccgagag caucgucauc     2280

gagauggccc gggagaacca gaccaccgcc cgcggcaaga agaacuccca gcagcgguac     2340

aagcgcaucg aggacgcccu gaagaaccug gcgcccgggc ucgacagcaa cauccugaag     2400

gaguacccca ccgacaacau ccagcugcag aacgaccggc uguuccugua cuaccugcag     2460

aacggcaagg acauguacac gggggagccg cucgacauca accagcuguc cagcuacgac     2520

aucgaccaca ucgugcccca ggccuucauc aaggacgacu cccuggacaa ccgcgugcuc     2580

accagcucca aggacaaccg gggcaagagc gacaacgucc ccucccugga gguggugcag     2640

aagcgcaagg ccuucuggca gcagcugcuc gacagcaagc ugaucuccga gcggaaguuc     2700

aacaaccuga ccaaggccga gcgcggcggg cucgacgagc gcgacaaggu cggcuucauc     2760

cggcgccagc ugguggagac ccggcagauc accaagcacg uggcccagau ccuggacgcg     2820

cgcuucaaca cggaggucac cgagaaggac aagaagaacc ggaacgugaa gaucaucacc     2880

cucaagagca accugguguc caacuuccgc aaggaguuca agcuguacaa gguccgggag     2940

aucaacgacu accaccacgc ccacgacgcc uaccucaacg ccgugguggc gaaggccauc     3000

cugaagaagu accccaagcu ggagccggag uucgucuacg gggacuacca gaaguacgac     3060

cucaagcgcu acaucagccg guccaaggac cccaaggacg uggagaaggc caccgagaag     3120

uacuucuucu acagcaaccu gcugaacuuc uucaaggagg aggugcacua cgccgacggc     3180

acgaucguca agcgcgagaa caucgaguac uccaaggaca ccggggagau cgccuggaac     3240

aaggagaagg acuucgcgac caucaagaag gugcucagcc ugccccaggu gaacaucguc     3300

aagaagaccg agauccagac gcacggccug gaccggggca agccccgcgg ccucuucaac     3360

agcaacccgu cccccaagcc cagcgaggac uccaaggaga accuggugcc gaucaagcag     3420

gggcuggacc cccggaagua cgggggcuac gccgggauca gcaacuccua cgccgugcuc     3480

gucaaggcca ucaucgagaa gggcgccaag aagcagcaga agaccgugcu ggaguuccag     3540

ggcaucagca uccuggacaa gaucaacuuc gagaagaaca aggagaacua ccuccuggag     3600

aagggguaca ucaagauccu guccaccauc acccucccca aguacagccu guucgaguuc     3660

cccgacggca cgcgccggcg ccuggcgucc auccucagca ccaacaacaa gcgcggggag     3720

auccacaagg gcaacgagcu ggugaucucc gagaaguaca ccacccugcu cuaccacgcc     3780

aagaacauca acaagacgcu ggagccggag caccuggagu acgucgagaa gcaccggaac     3840

gacuucgcca agcuccugga gagcgugcug gacuucaacg acaaguacgu gggggcccuc     3900

aagaacggcg agcggauccg ccaggccuuc aucgacuggg agaccgucga caucgagaag     3960

cugugcuucu ccuucaucgg cccccggaac agcaagaacg cggggcuguu cgagcucacc     4020

ucccagggca gcgccuccga cuucgaguuc cuggggguga agaucccccg cuaccgcgac     4080

uacaccccca gcucccugcu caacgccacg cugauccacc agagcaucac cggccuguac     4140

gagacccgga ucgaccucag caagcugggg gaggacuga                            4179


<210>  2098
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2098
augaacaaca acaacuacag caucggccug gacaucggga ccaacuccgu gggcugggcc       60

gucaucaccg acgacuacaa ggugcccagc aagaagauga aggugcucgg gaacacggac      120

aagcacuuca ucaagaagaa ccugaucggc gcccuccucu ucgacgaggg ggcgaccgcc      180

gaggaccgcc gguucaagcg caccgcccgg cgccgguaca cccgccggaa gaaccgccug      240

cgguaccugc aggagaucuu cuccgaggag augagcaagg ucgacuccag cuucuuccac      300

cgccucgacg acuccuuccu ggugccggag gacaagcggg gcagcaagua ccccaucuuc      360

gccacgcugg ccgaggagaa ggaguaccac aagaaguucc ccaccaucua ccaccuccgc      420

aagcaccugg cggacuccaa ggagaagacc gaccugcggc ucaucuaccu ggcccuggcc      480

cacaugauca aguaccgcgg ccacuuccuc uacgaggaga gcuucgacau caagaacaac      540

gacauccaga agaucuucuc cgaguucauc agcaucuacg acaacaccuu cgaggggucc      600

agccuguccg gccagaacgc ccagguggag gcgaucuuca cggacaagau cagcaagucc      660

gccaagcggg agcgcauccu gaagcuguuc gccuacgaga aguccaccga ccuguucagc      720

gaguuccuga agcucaucgu cgggaaccag gccgacuuca agaagcacuu cgaccuggag      780

gagaaggcgc cccugcaguu cagcaaggac accuacgacg aggaccucga gaaccugcug      840

ggccagaucg gggacgacuu cgccgaccuc uuccuggugg ccaagaagcu guacgacgcc      900

auccuccuga gcggcauccu gaccgugacg gacuccagca ccaaggcccc gcucuccgcc      960

agcaugaucg agcgguacga gaaccaccag aaggaccugg cggcccugaa gcaguucauc     1020

cagaacaacc uccaggagaa guacgacgag gucuucuccg accagagcaa ggacggcuac     1080

gcccgcuaca ucaacgggaa gaccacccag gaggccuucu acaaguacau caagaaccug     1140

cuguccaagu ucgagggcag cgacuacuuc cucgacaaga ucgagcggga ggacuuccug     1200

cgcaagcagc ggacguucga caacgggucc aucccccacc agauccaccu ccaggagaug     1260

aacgccauca uccgccgcca gggcgagcac uaccccuucc ucaaggagua caaggagaag     1320

aucgagacca uccugaccuu ccggaucccc uacuacgugg gcccgcuggc gcgcgggaac     1380

cggaacuucg ccuggcucac ccgcaacagc gaccaggcca uccggcccug gaacuucgag     1440

gagaucgugg accaggccuc cagcgccgag gaguucauca acaagaugac gaacuacgac     1500

cuguaccugc ccgaggagaa gguccucccc aagcacuccc ugcuguacga gaccuucgcg     1560

guguacaacg agcugaccaa ggugaaguuc aucagcgagg gccuccgcga cuaccaguuc     1620

cuggacuccg ggcagaagaa gcagaucguc aaccagcugu ucaaggagaa gcggaaggug     1680

accgagaagg acaucaucca guaccuccac aacguggacg gcuacgacgg gaucgagcug     1740

aagggcaucg agaagcaguu caacgccagc cuguccacgu accacgaccu ccugaagauc     1800

aucaaggaca aggaguucau ggacgacccg aagaacgagg agauccugga gaacaucguc     1860

cacacccuca ccaucuucga ggaccgcgag augaucaagc agcggcuggc ccaguacgcc     1920

agcaucuucg acaagaaggu gaucaaggcg cugacccgcc ggcacuacac gggcuggggg     1980

aagcucuccg ccaagcugau caacggcauc ugcgacaaga agaccgggaa gaccauccug     2040

gacuaccuca ucgacgacgg cuacagcaac cgcaacuuca ugcagcugau caacgacgac     2100

gggcugagcu ucaaggacau cauccagaag gcccaggugg ucggcaagac caacgacgug     2160

aagcaggucg ugcaggagcu ccccggcucc cccgccauca agaaggggau ccugcagagc     2220

aucaagcugg uggacgagcu cgucaaggug augggccacg cccccgaguc caucgugauc     2280

gagaucgcgc gggagaacca gacgaccgcc cgcgggaaga agaacagcca gcagcgguac     2340

aagcgcaucg aggacgcccu gaagaaccug gccccgggcc ucgacuccaa cauccugaag     2400

gagcacccca ccgacaacau ccagcugcag aacgaccggc uguuccugua cuaccugcag     2460

aacgggaagg acauguacac cggcgaggcc cucgacauca accagcugag cuccuacgac     2520

aucgaccaca ucguccccca ggcguucauc aaggacgaca gccuggacaa ccgcgugcuc     2580

acguccagca aggacaaccg gggcaagucc gacaacgugc ccagccugga ggucgugcag     2640

aagcgcaagg ccuucuggca gcagcugcuc gacuccaagc ugaucagcga gcacaaguuc     2700

aacaaccuga ccaaggccga gcgcgggggc cucgacgagc gggacaaggu gggguucauc     2760

cgccggcagc uggucgagac ccgccagauc accaagcacg uggcccagau ccuggacgcc     2820

cgguucaaca ccgaggugaa cgagaaggac aagaagaacc gcacggucaa gaucaucacc     2880

cucaagucca accuggugag caacuuccgg aaggaguuca agcuguacaa ggugcgcgag     2940

aucaacgacu accaccacgc gcacgacgcc uaccucaacg ccgucguggc caaggcgauc     3000

cugaagaagu acccgaagcu ggagcccgag uucguguacg gcgacuacca gaaguacgac     3060

aucaagcggu acaucucccg cagcaaggac cccaaggagg ucgagaaggc caccgagaag     3120

uacuucuucu acagcaaccu ccugaacuuc uucaaggagg aggugcacua cgccgacggc     3180

accaucguga agcgggagaa caucgaguac uccaaggaca cgggggagau cgccuggaac     3240

aaggagaagg acuucgccac caucaagaag guccugagcc ucccccaggu gaacaucgug     3300

aagaagaccg agauccagac ccacggccug gaccgcggga agccgcgggg ccuguucaac     3360

uccaacccca gccccaagcc cuccgaggac cgcaaggaga accucguccc gaucaagcag     3420

gggcuggacc cccggaagua cggcggcuac gcggggauca gcaacuccua cgccgugcug     3480

gugaaggcca ucaucgagaa gggcgccaag aagcagcaga agacgguccu cgaguuccag     3540

gggaucagca uccuggacaa gaucaacuuc gagaagaaca aggagaacua ccugcucgag     3600

aagggcuaca ucaagauccu guccaccauc acccugccca aguacagccu cuucgaguuc     3660

ccggacggga cccgccggcg ccuggccucc auccugagca cgaacaacaa gcggggcgag     3720

auccacaagg gcaacgagcu cgugaucccc gagaaguaca ccacccugcu guaccacgcg     3780

aagaacauca acaagacccu cgagcccgag caccuggagu acguggagaa gcaccgcaac     3840

gacuucgcca agcugcucga guacguccug aacuucaacg acaaguacgu gggggcccug     3900

aagaacggcg agcggauccg ccaggccuuc aucgacuggg agacggugga caucgagaag     3960

cucugcuucu ccuucaucgg gccccgcaac agcaagaacg ccggccuguu cgagcugacc     4020

ucccagggga gcgcguccga cuucgaguuc cucggcguca agaucccgcg guaccgcgac     4080

uacaccccca gcucccugcu gaacgccacc cucauccacc agagcaucac gggccuguac     4140

gagacccgga ucgaccugag caagcucggg gaggacuga                            4179


<210>  2099
<211>  4182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2099
augaagcgcg ugaccgagga cuacauccug ggccucgaca ucgggaccaa cagcugcggc       60

ugggccguca cggacaagaa gaacaaccug cugaagcucc gggggaagac cgccaucggc      120

ucccaccugu ucgaggaggg gcacaccgcg gccgaccgcc ggggcuuccg caccacgcgg      180

cgccggcuga agcgccggaa guggcgccuc cggcugcugg aggagaucuu cgccgagccc      240

auggccaagg uggacccggg cuucuucgug cgccuccacc agagcugggu cagcccccug      300

gacaagagcc ggaagaagua ccacgccauc guguucccca ccgcgaagga ggacaaggcc      360

uucuaccaga aguaccccac caucuaccac cuccgcgacg cccucaugac ccaggaccgg      420

caguucgacc ugcgcgagau cuuccuggcc auccaccaca ucgugaaguu ccgggggaac      480

uuccuccagg acacgccggu caaggacuuc gaggcgucca agaucgaggu gggccccauc      540

cugagcaaga ucaacggggc cuucgccgag aagaucgugg aggaccagga ccccaucgag      600

cugaacgucg ccaacgccgc ggagaucgag gccgugaucc gcggcaagga cgccgacaag      660

acccucuaca agauggacaa ggugaagaag aucgccaagc ugcugaccga cuccaccgcc      720

aaggaggaga agaacgucgc gaagcagauc gccaacgccg ugauggggua caagacgcag      780

uucgagacca uccucggcaa ggagaucgac aagagcgaca aggcccagug ggaguucaag      840

cugaccgacg gcgacgccga cgacaagcug gaggcgcucc ugcccgaccu ggacgagaac      900

gaccagacca ucaucgccga gaucaagaag cuguucuccg ccaucacgcu gagcaccauc      960

guggacgaga acaagucccu gagccagucc auggucgcca aguacaacaa gcaccaccag     1020

gacuacaagc uccugaaggc cuacaucgac acccugcagg accggagcau cgcgaagaag     1080

cuccugcugg ccuacgaccu cuacgugaac aaccgccacg ggcggcugcu ggaggccaag     1140

accgaguuca agcaggacgg cacgcucacc aaggaggacu ucuacaagac cgugaaggac     1200

aaccuggacg acuccgagcu ggcccgcaag aucgcgcaga agaucgccgc cgacaacuuc     1260

augccgaagc agcgcaccaa cagcaacggg gucauccccu uccagcucca ccagaucgag     1320

cuggacaaga ucaucgccaa ccaguccaag uacuaccccu uccuggccgc ggagaacccc     1380

guggaggacc accggaagca ggccccguac aagcucgacg agcuggugcg cuuccggguc     1440

cccuacuacg ugggccccau gaucacgccc gacgagcagg agaagaccag cggcaagucc     1500

uucgccugga uggugcgcaa ggaggacggg cagaucaccc cguggaacuu cgagcagaag     1560

gucgaccggc aggagagcgc caacaaguuc aucaagcgca ugaccaucaa ggacacguac     1620

cugcucggcg aggacgugcu gcccgccaac agccugcugu accagcgguu cgagguccuc     1680

aacgagcuga acaacauccg caucaacggg ucccggauca gcaucgaccu gaagcagcag     1740

aucuucaacg accucuucga ggagaagaag accgugaccg agaagucccu gaccagcuac     1800

cugaagcaga accuccaccu ccccacggug gagaucaagg gccuggcgga ccccaccaag     1860

uucaacucca gccuggccuc cuacuaccac cucaagagcc ugcacgucuu cgacaaggag     1920

cuggccgacc cgcaguacca gaaggacuuc gagaagauca ucgaguacuc cagcaucuuc     1980

gaggacaaga agaucuacca ggacaagcug cacgccgagu ucaaguggcu gacccccgag     2040

caguucaagg ccaucuccac cuggcgccuc cagggguggg gccggcugag cugccgccug     2100

cucguggagc ugcacgacac gaacggccag aacaucaugg agcagcugug ggacucccag     2160

aagaacuuca ugcagaucgu gaccgagccc gacuucaagg acgcgaucgc caaggagaac     2220

cagaacguca cccaggugaa cgggguggag gagauccucg ccgacgccua caccagcccc     2280

gcgaacaaga aggccauccg gcaggucgug aagguggucg ccgacguggu gaaggccgcc     2340

ggcgggaaga agccggcgca guucgccauc gaguucaccc gcgaccccga caagaacccc     2400

cagcuguccc acauccgggg cacgaagcug cucaaggccu accaggagac cgccggggag     2460

cuggucgacc agaagcugac cgacagccuc aaggaggcca ugaccucccg caagcugcug     2520

aaggacaagu acuuccucua cuucaugcag acgggccggg acgcguacac cggcaagaag     2580

aucaacaucg acgaggugac caccaacuac cagaucgacc acauccugcc ccagagcuuc     2640

aucaaggacg acuccuucga caaccgcgug cugacggcca ccccgcucaa cgccgagaag     2700

uccgacgacg uccccuacaa gcgguucgcc aacaacuacg ugagcgaccu gaagaugacc     2760

gugggggaga uguggaagca cuggcagaag gccggcauca ucaacaagca caagcugggg     2820

aaccuccugc uggaccccga ccgccucaac aaguuccaga aguccggcuu caucaaccgc     2880

cagcuggucg agaccagcca gaucgugaag cugguguccg ucauccucca gaacaaguac     2940

ccggacgcgg agaucaucac ggugaaggcc ggggacaaca gcgcccugcg gcagcgccug     3000

aaccucuaca agucccggga cgugaacgac uaccaccacg ccaucgacgc guaccugagc     3060

aucaucugcg gcaacuuccu guaccagguc uaccccaagu accgccccua cuucguguac     3120

ggcaaguaca agaaguucuc ccaggacccc gaccuccaga aggaccugau caagcacuuc     3180

aagggguuca ccuucaugug gccgcugcuc cagaaggaca acagcgagcg gaaggccccc     3240

gaggagauca aggagaacaa cuccgagcgc aucguguucu acaagcaccc cgacaucuuc     3300

gacaagcugc ggaaggccua caacuacaag uacaugcugg ucagccgcga gaccaccacg     3360

gaguccagcu ccauguucaa gaugaccauc uucccccggg gcgagcgcga ccggaagaag     3420

acccgcaagc ucaucccgaa ggggaacggc cuggacccca agaucuacgg cggguacagc     3480

ggcaacaccg acgccuacau ggugaucgug aagaucgaca aggccaagga guccaucuac     3540

aaggucaucg gggugcccau gcgggcgcug acgcgccuca accaggccaa gaagcagggc     3600

aacuaccagg aggagcugca ccaggugcug gagccccaga ucaugcucga caagaacggg     3660

aagccgaagc ggggcgucaa gggcuuccgc gugaucaagg accacgugcc cuucaagcag     3720

gucgugcugg acggggacaa gaaguucaug cuguacagcg cccaguacaa gaccaacgcc     3780

aagcagcucg cccuguccca ggagaccaug cggaucguga ccgacaaccu gaagaagggc     3840

gaggaccagg acaagcuccu ggucaaggcg uacgacgaga uccugcagaa gguggacugc     3900

uaccucagcc uguucgacac gagcaacuuc cgcaacgggc ugcaccucgg ccggaacaag     3960

uucaucgacc uggaggugaa cgacaagaag aucacccugu ccaacauccu caacgggcug     4020

cacgacaaca ugguccuggg caaccucaag aacaucggca ucgggacgcc ccugggccag     4080

augcaggaga aguccgggau cacccugagc cccgaggccg ugcucaucuu ccagagcccg     4140

accggccugu ucgagaagcg cgugaagauc gccgaccugu ga                        4182


<210>  2100
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2100
augaagaagg agaucaagga cuacuuccug ggccucgacg uggggaccgg cagcgucggg       60

ugggccguga ccgacacgga cuacaagcug cugaaggcca accgcaagga ccucuggggc      120

augcggugcu ucgagaccgc ggagaccgcc gaggugcgcc ggcugcaccg cggggcccgg      180

cgccggaucg agcgccggaa gaagcgcauc aagcugcucc aggagcuguu cucccaggag      240

aucgccaaga ucgacgaggg cuucuuccag cggaugaagg agagccccuu cuacgccgag      300

gacaagacca uccugcagga gaacgcccuc uucaacgaca aggacuucac ggacaagacc      360

uaccacaagg cguacccgac caucaaccac cugaucaagg ccuggaucga gaacaagguc      420

aagcccgacc cccgccugcu cuaccuggcc ugccacaaca ucaucaagaa gcggggccac      480

uuccuguucg agggggacuu cgacuccgag aaccaguucg acaccagcau ccaggcccuc      540

uucgaguacc ugcgcgagga cauggaggug gacaucgacg cggacuccca gaaggugaag      600

gagauccuga aggacagcuc ccucaagaac agcgagaagc agucccggcu gaacaagauc      660

cugggccuca agcccagcga caagcagaag aaggccauca cgaaccugau cuccgggaac      720

aagaucaacu ucgccgaccu guacgacaac ccggaccuca aggacgccga gaagaacagc      780

aucuccuuca gcaaggacga cuucgacgcg cuguccgacg accuggccag cauccucggc      840

gacuccuucg agcugcugcu caaggccaag gccgucuaca acugcagcgu gcuguccaag      900

gugaucgggg acgagcagua ccugagcuuc gccaagguca agaucuacga gaagcacaag      960

accgaccuca ccaagcugaa ggcggugauc aagaagcacu uccccaacaa cuacaagaag     1020

guguucggcu acaaccagga caacgagagc aagaacaacu acuccggcua cgucggggug     1080

ugcaagacca agaacaagaa gcuggugauc aacaacagcg ucaaccagga ggacuucuac     1140

aaguuccuca agcccauccu guccgccaag agcgagauca aggaggugaa cgacauccug     1200

gccgagaucg agacgggcaa cuuccucccc aagcagaucu ccaagagcaa cgccgagauc     1260

ccguaccagc ugcgcaagau ggagcuggag aagauccucu ccaacgccga gaagcacuuc     1320

agcuuccuga agcagaagga cgagaagggg cugucccaca gcgagaagau caucaugcuc     1380

cugaccuuca agauccccua cuacaucggc cccaucaacg acaaccacaa gaaguccuuc     1440

cccgaccggu gcuggguggu caagaaggag aaguccccga gcggcaagac cacccccugg     1500

aacuucuucg accacaucga caaggagaag acggcggagg aguucaucac cagccgcacc     1560

aacuucugca ccuaccugau cggggagucc gugcucccca agaacagccu gcuguacucc     1620

gaguacacgg ugcugaacga gaucaacaac cuccagauca ucaucgacgg caagaacauc     1680

ugcgacacca agcugaagca gaagaucuac gaggaccugu ucaagaagua caagaagauc     1740

acccagaagc agaucagcac cuucaucaag cacgagggga ucugcaacaa gacggacgag     1800

gucaucaucc ucggcaucga caaggagugc accuccagcc ucaagagcua caucgagcug     1860

aagaacaucu ucgggaagca ggucgacgag aucuccacca agaacaugcu cgaggagauc     1920

auccgguggg ccaccaucua cgacgagggc gagggcaaga cgauccugaa gaccaagauc     1980

aaggccgagu acgggaagua cugcagcgac gagcagauca agaagauccu gaaccucaag     2040

uucuccggcu gggggcgccu gagccggaag uuccuggaga ccgugaccuc cgagaugccc     2100

ggcuucagcg agccggugaa caucaucacg gccaugcgcg agacccagaa caaccucaug     2160

gagcugcugu ccagcgaguu caaguucacc gagaacauca agaagaucaa cuccggguuc     2220

gaggacgccg agaagcaguu cagcuacgac ggccucguga agccccuguu ccuguccccc     2280

agcgucaaga agaugcucug gcagacccug aagcugguga aggagaucuc ccacaucacg     2340

caggcgcccc cgaagaagau cuucaucgag auggccaagg gcgccgagcu cgagcccgcc     2400

cgcaccaaga cccggcugaa gauccugcag gaccucuaca gcuccugcaa gaacgacgcg     2460

gacgccuuca gcuccgagau ccaguaccug agcgggaaga ucgagaacga ggacaaccug     2520

cgccuccgga gcgacaagcu guaccuguac uacacccagc ucggcaagug cauguacugc     2580

gggaagccca ucgagaucgg ccacguguuc gacaccucca acuacgacau cgaccacauc     2640

uacccccaga gcaagaucaa ggacgacucc aucagcaacc gcguccuggu gugcuccagc     2700

ugcaacaaga acaaggagga caaguacccg cugaaguccg agauccagag caagcagcgg     2760

ggguucugga acuuccucca gcgcaacaac uucaucuccc uggagaagcu gaaccggcuc     2820

acgcgcacca cccccaucag cgacgacgag accgccaagu ucaucgcccg gcagcuggug     2880

gagacgcgcc aggccaccaa ggucgcggcc aaggugcugg agaagauguu ccccgagacc     2940

aagaucgugu acuccaaggc cgagaccguc agcauguucc ggaacaaguu cgacaucauc     3000

aagugccgcg agaucaacga cuuccaccac gcccacgacg ccuaccucaa caucguggug     3060

ggcaacgucu acaacacgaa guucaccaac aaccccugga acuucaucaa ggagaagcgg     3120

gacaacccga agaucgcgga caccuacaac uacuacaagg uguucgacua cgacgugaag     3180

cgcaacaaca ucaccgccug ggagaagggc aagacgauca ucaccgucaa ggacaugcug     3240

aagcggaaca cccccaucua cacccgccag gccgccugca agaagggcgg gcuguucgac     3300

cagacgauca ugaagaaggg gcucggccag cacccccuga agaaggaggg cccguucucc     3360

aacaucagca aguacggggg cuacaacaag guguccgccg cguacuacac ccugaucgag     3420

uacgaggaga aggggaacaa gauccggagc cucgagacca ucccccugua ccuggugaag     3480

gacauccaga aggaccagga cguccucaag uccuaccuga ccgaccugcu cggcaagaag     3540

gaguucaaga uccuggugcc caagaucaag aucaacagcc ugcucaagau caacggguuc     3600

cccugccaca ucacgggcaa gaccaacgac uccuuccugc ugcgcccggc cgugcaguuc     3660

ugcugcagca acgacgaggu ccucuacuuc aagaagauca uccgguucag cgagauccgc     3720

ucccagcgcg agaagaucgg caagaccauc agccccuacg aggaccuguc cuuccggagc     3780

uacaucaagg agaaccugug caagaagacc aagaacgacg agaucgggga gaaggaguuc     3840

uacgaccucc ugcagaagaa gaaccuggag aucuacgaca ugcuccugac gaagcacaag     3900

gacaccaucu acaagaagcg ccccaacucc gccaccaucg acauccuggu gaagggcaag     3960

gagaaguuca agagccucau caucgagaac caguucgagg ugauccugga gauccugaag     4020

cuguucuccg ccacccggaa cgucagcgag cugcagcaca ucgggggcuc caaguacagc     4080

gggguggcga agaucggcaa caagaucucc agccuggaca acugcauccu caucuaccag     4140

uccaucacgg gcaucuucga gaagcgcauc gaccugcuga agguguga                  4188


<210>  2101
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2101
augaagaagg agaucaagga cuacuuccug ggccucgacg uggggaccgg cagcgucggg       60

ugggccguga ccgacacgga cuacaagcug cugaaggcca accgcaagga ccucuggggc      120

augcggugcu ucgagaccgc ggagaccgcc gaggugcgcc ggcugcaccg cggggcccgg      180

cgccggaucg agcgccggaa gaagcgcauc aagcugcucc aggagcuguu cucccaggag      240

aucgccaaga ucgacgaggg cuucuuccag cggaugaagg agagccccuu cuacgccgag      300

gacaagacca uccugcagga gaacacccuc uucaacgaca aggacuucac ggacaagacc      360

uaccacaagg cguacccgac caucaaccac cugaucaagg ccuggaucga gaacaagguc      420

aagcccgacc cccgccugcu cuaccuggcc ugccacaaca ucaucaagaa gcggggccac      480

uuccuguucg agggggacuu cgacuccgag aaccaguucg acacgagcau ccaggcccuc      540

uucgaguacc ugcgcgagga cauggaggug gacaucgacg cggacuccca gaaggugaag      600

gagauccuga aggacagcuc ccucaagaac agcgagaagc agucccggcu gaacaagauc      660

cugggccuca agcccagcga caagcagaag aaggccauca ccaaccugau cuccgggaac      720

aagaucaacu ucgccgaccu guacgacaac ccggaccuca aggacgccga gaagaacagc      780

aucuccuuca gcaaggacga cuucgacgcc cuguccgacg accuggcgag cauccucggc      840

gacuccuucg agcugcugcu caaggccaag gccgucuaca acugcagcgu gcuguccaag      900

gugaucgggg acgagcagua ccugagcuuc gccaagguca agaucuacga gaagcacaag      960

accgaccuca ccaagcugaa gaacgugauc aagaagcacu uccccaagga cuacaagaag     1020

guguucggcu acaacaagaa cgagaagaac aacaacaacu acagcggcua cgucggggug     1080

ugcaagacga aguccaagaa gcugaucauc aacaacagcg ugaaccagga ggacuucuac     1140

aaguuccuca agaccauccu guccgccaag agcgagauca aggaggucaa cgacauccug     1200

accgagaucg agaccggcac guuccucccc aagcagaucu ccaagagcaa cgcggagauc     1260

cccuaccagc ugcgcaagau ggagcuggag aagauccucu ccaacgccga gaagcacuuc     1320

agcuuccuga agcagaagga cgagaagggg cugucccaca gcgagaagau caucaugcuc     1380

cugaccuuca agaucccgua cuacaucggc cccaucaacg acaaccacaa gaaguccuuc     1440

cccgaccggu gcugggugau caagaaggag aaguccccca gcggcaagac caccccgugg     1500

aacuucuucg accacaucga caaggacaag acggccgagg ccuucaucac cagccgcacc     1560

aacuucugca ccuaccuggu gggggagucc guccucccca agagcucccu gcuguacagc     1620

gaguacacgg ugcugaacga gaucaacaac cuccagauca ucaucgacgg caagaacauc     1680

ugcgacauca agcugaagca gaagaucuac gaggagcugu ucaagaagua caagaagauc     1740

acccagaagc agaucuccac cuucaucaag cacgagggga ucugcaacaa gaccgacgag     1800

gugaucaucc ucggcaucga caaggagugc acgagcagcc ugaaguccua caucgagcug     1860

aagaacaucu ucgggaagca ggucgacgag aucagcacca agaacaugcu cgaggagauc     1920

auccgguggg ccaccaucua cgacgagggc gagggcaaga ccauccugaa gacgaagauc     1980

aaggcggagu acgggaagua cugcuccgac gagcagauca agaagauccu gaaccucaag     2040

uucagcggcu gggggcgccu gucccggaag uuccuggaga ccgugaccag cgagaugccc     2100

ggcuucuccg agcccgugaa caucaucacc gccaugcgcg agacgcagaa caaccucaug     2160

gagcugcuga gcuccgaguu caaguucacc gagaacauca agaagaucaa cagcggguuc     2220

gaggacgccg agaagcaguu cuccuacaac ggccucguca agccgcuguu ccugagcccc     2280

uccgugaaga agaugcucug gcagacccug aagcugguca aggagaucag ccacaucacc     2340

caggccccgc ccaagaagau cuucaucgag auggccaagg gcgcggagcu cgagcccgcc     2400

cgcaccaaga cgcggcugaa gauccugcag gaccucuaca acaacugcaa gaacgacgcc     2460

gacgccuucu ccagcgagau caaggaccug uccgggaaga ucgagaacga ggacaaccug     2520

cgccuccgga gcgacaagcu guaccuguac uacacccagc ucggcaagug cauguacugc     2580

gggaagccca ucgagaucgg ccacguguuc gacaccagca acuacgacau cgaccacauc     2640

uacccccagu ccaagaucaa ggacgacagc aucuccaacc gcgugcuggu cugcagcucc     2700

ugcaacaaga acaaggagga caaguacccc cucaagagcg agauccaguc caagcagcgg     2760

ggguucugga acuuccucca gcgcaacaac uucaucagcc uggagaagcu gaaccggcuc     2820

acccgcacga ccccgaucuc cgacgacgag accgcgaagu ucaucgcccg gcagcuggug     2880

gagacccgcc aggccacgaa gguggccgcc aagguccugg agaagauguu ccccgagacc     2940

aagaucgugu acagcaaggc ggagaccgug uccauguucc ggaacaaguu cgacaucauc     3000

aagugccgcg agaucaacga cuuccaccac gcccacgacg ccuaccucaa caucgucgug     3060

ggcaacgugu acaacaccaa guucacgaac aaccccugga acuucaucaa ggagaagcgg     3120

gacaacccca agaucgccga caccuacaac uacuacaagg ucuucgacua cgacgugaag     3180

cgcaacaaca ucaccgccug ggagaagggc aagaccauca ucacggugaa ggacaugcug     3240

aagcggaaca ccccgaucua cacccgccag gcggccugca agaagggcgg gcuguucgac     3300

cagaccauca ugaagaaggg gcucggccag cacccccuga agaaggaggg cgccuucagc     3360

aacaucucca aguacggggg cuacaacaag gucagcgccg ccuacuacac gcugaucgag     3420

uacgaggaga aggggaacaa gauccggucc cucgagacca ucccccugua ccuggugaag     3480

gacauccaga aggaccagga cgugcucaag agcuaccuga ccgaccugcu cggcaagaag     3540

gagaucaaga uccugguccc gaagaucaag aucaacuccc ugcucaagau caacggguuc     3600

cccugccaca ucaccggcaa gacgggcgac agcuucgugc ugcgccccgc ggugcaguuc     3660

ugcugcucca acgacgaggu ccuguacuuc aagaagauca uccgguucaa cgagauccgc     3720

agccagcgcg agaagauggg gaagaccauc agccccuacg aggaccucuc cuuccggagc     3780

uacaucaagg agaaccugug caagaagacc aaggccgacg agaucggcga gaaggaguuc     3840

uacgaccugc uccagaagaa gaaccuggag aucuacgaca ugcugcucac caagcacaag     3900

gacacgaucu acaacaagcg cccgaacucc accacccugg ggaugcugac caagggccgg     3960

uacgaguuca ucaaccucaa gcccaaggac cagaucaucg ugaugcugga gauccugaag     4020

cuguucugca cgacccgcga ggccaucgac cuggggcuga ucaagggcaa gcccgccgcg     4080

ggcguggcca cccucgggaa gaagaucagc aaccccgaca acugcauccu gaucuaccag     4140

uccaucaccg gcaucuucga gaagcggauc gaccugcuca aggucuga                  4188


<210>  2102
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2102
augggcgaca ccgugaagaa cuaccacauc gggcuggaca ucggcaccag cuccgucggg       60

uucgccgccc gcgacgacga caacaaccuc auccggguga agggcaagaa cgugaucggg      120

acccgccugu ucagcgaggg ccagaccgcg caggagcggc gcauggcccg ggccucccgc      180

cggcgcuaca accggcgcaa guggcggcug cgccuccugg acgagaucuu ccacgacgag      240

aucauccaca aggacgccga guucuucaac cggcugaagc agagcucccu cagccccaag      300

gacgagcgca agcaguacuu cggcucccug cuguucccgg aggucgggga cagcgaguac      360

uaccacgacg gcaaccagac caucuaccac cuccggcacc gccugaugac ccaggacgag      420

aaggccgaca uccgggagau cuaccuggcg cuccgccaca ucaucaagua ccgggggaac      480

uuccuggacg acacgcccgu guccagcuuc gaggccuccg accugaagcu cgccagcuuc      540

gugcccaccc ugaacgaccu guucgagacc aucgacguca acuacgaguu cuccauggac      600

aacaucaacg agaucgagga cauccuccug aacaacaagc ugcgcaacag cgacaagucc      660

aagcagcucc ccgaccugcu gaccgagccg aucgugaaca ucgacgacaa gagccucgag      720

aagcaguuca acaaggagcg gacggacauc uccaaggaga ucgccaagag cguggucggc      780

uccaagauga acgugaacaa gcugcugagc uucgagagcu ccgggggcua caagaucgcg      840

uucagcuucu ccgacgccag cuccgaggac aagaugaugg agcucagcga cgugcuggac      900

gacucccgca ugaacauccu gaacauccuc aaggucaucu acagccgcgu gcggcugaac      960

cagaucaucc ccuccggcau ggggcugagc gaguccaugg uggccgccua cgaccuccac     1020

aagcagcagc ugaaggaccu gaagaagcuc cugaacgucc ugcccuucaa cgagcgcuac     1080

gccgugaaga ucgcguacgc cgccuacauc ggcaacaucg acaagcagaa guucaccaag     1140

agcgagguga agaccaucga caagcucaag gagaaguccg ccaucaugaa cguccuggac     1200

uucaagagcg ggcacaucac caacgaggag cuguccaacg ugaucaaggu ggucaucgug     1260

gacaaguacc ucaacgacga gaacuucgag uaccacagca ucguggagaa gaucaaccag     1320

gacaucucca acggcagcua ccugcugaag cagcggaaca accagaacgg caacaucccc     1380

caccagcucc accagaugga gcuggaccag aucaucgaga accaguccaa guacuacccg     1440

uuccuggggg agcucaaccc caacagcaac cgcaaccaca ucgccaagua caagaucagc     1500

gagcuggucg cguuccgggu gcccuacuac gucggccccc ugaucacgcc ggaggagcag     1560

gagaagaccu ccggggccag cuucgccugg augaagcgca aggaggacgg cgccaucacc     1620

cccuggaacu ucgaggagaa gguggacaag gaggagaccg ccucccgguu caucaagcgc     1680

cugacgguga aggacaccua ccuccugaac gaggacgucc ugcccgacaa cagccuccug     1740

uacgagaagu ucaaggugcu gaacgagcuc aacgugguca aggcgaacaa caagcggcug     1800

accguggagg agaagcaggg ggccuucgac aaccuguuca agaagaugaa gaccgugacg     1860

gccaagaagc ucaagaacua ccuggucucc aaccacaacc acaucaacag cguggagauc     1920

accggccugu ccgacgugga caaguucaac aacagccucu ccaccuacau cgacuucaag     1980

aagaucuucg acgacaagau cgacaacccc gaccugcugg acgacuucga ggacaucauc     2040

gaguggagca ccgucuucga ggaccgcaag aucuucgagg agaaggugcg gaaggacauc     2100

uccuggauca gcgaguccga gaucaaggcc guggucagca agcgcuacuc cggcuggggg     2160

cggcucagca agaagcugcu gaacggcauc acgaacaacg aggggcgcaa caucaucgac     2220

gagcucuggg aggaguccga gaccuucaug gaggccguga gcaagccgga ggugaaggag     2280

cagaucgacg gcaucaacag cgacuucauc aagaacaccg acaucgaguc cguccuggcg     2340

gacgccuaca ccagccccca gaacaagaag gccauccggg agacgcacaa gcuggugaag     2400

gacauccaga aggccauggg cgggcaggcg cccgccucca ucagcaucga guucacccgc     2460

aacccccagg acaagggcca gcucacccgg ucccgccaca gcgagguguc ccggaaguac     2520

aaggagauca gcaaggaggu cuccaagacc cugaacgacg agcucaagag caagaagaac     2580

uccauggacg uggacaagua cuaccuguac uucaugcaga uggggaucga ccuguacgac     2640

ggcagcucca ucgacaucga cgaccugaac aacuaccaga ucgaccacau caucccgcag     2700

agcuucuaca aggacgacuc cuucgacaac cgcgugcuca cgcacuacag caacaaccag     2760

aagaagggga acaacacgcc ccugaacggc cugaacaucg acgacaagac ccgcgccauc     2820

uggaaccacc uccgggaccu ggggcugauc accaagaaga aguacaacgg ccucacccug     2880

aagggcaugg accagcuguc caaguacacc aagcacgggu ucauccgccg gcagcucguc     2940

gagaccagcc aggugaucaa gcugguggcc aacauccugu ccgacgaguu cagcaccgac     3000

gacaccaaga ucaucgaggu caaggccaag augaacagcc agauccgcca gguguucaac     3060

cucuacaagg ugcgggagcu gaacgacuac caccacgcgg ucgacgccua ccugacgacc     3120

uucaucggcg gguaccucua caaccgcuac cccaaccugc ggaacauguu cguguacggc     3180

gacuucaaga aguucuccgc cgacguggac aaccugaaga ccuucaacuu ccuccacgac     3240

aucaccaacc ccagcgacaa guucgaggac cgcaucuucg acgagaaguc cggcgagcug     3300

auccuggacc ggaagcacgc caucgagacg aucaagaaga ucuaccagua caaguacaug     3360

cucaucaccc gcgaggucag caccaagaac gggcagcugu ccgaccagac cguguacagc     3420

gccaagcgga ucaacaacgu gaaggcgccg auccccguca agaaggacuc caaccccgag     3480

cuguacggcu uccgcacggg gaacguggac caccacaugg ugaucgucaa guaccccaag     3540

aaggacagcu acgacuacaa gguggugggc aucccgcucc gguucgucca gaagcugaag     3600

gagaccaagg acaacaagga cuacgagaac cugcucaacc agaucgugca gaugaagcug     3660

aaccgcaaga agcaggacuu caucgugguc guggacaacg ugauguacgg gcagcugauc     3720

caggacggcg gcgagaagua cacccucggg uccagcaccu acaaguacaa cgccaagcag     3780

cuggucauca acauggacaa gacgcggauc cuggaggaca accgcuaccu caacucccug     3840

gacgagggcg aggugagcga gaagcuggug aacaucuaca acgacaucau gaaggucgug     3900

aacucccggu ucgaccucuu cgacaucaac aaguuccgcg agaagcugaa cgacgggcug     3960

aacaaguuca agaacaucag caacgagcag aaggaggaga cccuccugaa gauccugucc     4020

ggccuccacu gcgggagcgc caacgucccc aucaaggagc ugggccugac cacgccccuc     4080

ggcaagcugc agcugccguc cgggaucgug cucagcgagg aggccguggu caucuaccag     4140

ucccccagcg gccuguucga gaacaaggug cggcugaagg accucuga                  4188


<210>  2103
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2103
augaagaagg agaucaagga cuacuuccug ggccucgacg uggggaccgg cagcgucggg       60

ugggccguga ccgacacgga cuacaagcug cugaaggcca accgcaagga ccucuggggc      120

augcggugcu ucgagaccgc ggagaccgcc gaggugcgcc ggcugcaccg cggggcccgg      180

cgccggaucg agcgccggaa gaagcgcauc aagcugcucc aggagcuguu cucccaggag      240

aucgccaaga ccgacgaggg cuucuuccag cggaugaagg agagccccuu cuacgccgag      300

gacaagacga uccugcagga gaacacccuc uucaacgaca aggacuucgc ggacaagacc      360

uaccacaagg ccuacccgac caucaaccac cugaucaagg ccuggaucga gaacaagguc      420

aagcccgacc cccgccugcu cuaccuggcc ugccacaaca ucaucaagaa gcggggccac      480

uuccuguucg agggggacuu cgacuccgag aaccaguucg acacgagcau ccaggcccuc      540

uucgaguacc ugcgcgagga cauggaggug gacaucgacg cggacuccca gaaggugaag      600

gagauccuga aggacagcuc ccucaagaac agcgagaagc agucccggcu gaacaagauc      660

cugggccuca agcccagcga caagcagaag aaggccauca ccaaccugau cuccgggaac      720

aagaucaacu ucgccgaccu guacgacaac ccggaccuca aggacgccga gaagaacagc      780

aucuccuuca gcaaggacga cuucgacgcg cuguccgacg accuggccag cauccucggc      840

gacuccuucg agcugcugcu caaggccaag gccgucuaca acugcagcgu gcuguccaag      900

gugaucgggg acgagcagua ccugagcuuc gccaagguca agaucuacga gaagcacaag      960

accgaccuca ccaagcugaa gaacgugauc aagaagcacu uccccaagga cuacaagaag     1020

guguucggcu acaacaagaa cgagaagaac aacaacaacu acagcggcua cgucggggug     1080

ugcaagacga aguccaagaa gcugaucauc aacaacagcg ugaaccagga ggacuucuac     1140

aaguuccuca agaccauccu guccgccaag agcgagauca aggaggucaa cgacauccug     1200

accgagaucg agaccggcac guuccucccc aagcagaucu ccaagagcaa cgcggagauc     1260

cccuaccagc ugcgcaagau ggagcuggag aagauccucu ccaacgccga gaagcacuuc     1320

agcuuccuga agcagaagga cgagaagggg cugucccaca gcgagaagau caucaugcuc     1380

cugaccuuca agaucccgua cuacaucggc cccaucaacg acaaccacaa gaaguucuuc     1440

cccgaccggu gcuggguggu gaagaaggag aaguccccca gcggcaagac caccccgugg     1500

aacuucuucg accacaucga caaggagaag acggccgagg ccuucaucac cucccgcacc     1560

aacuucugca ccuaccuggu cggggagagc gugcucccca aguccagccu gcuguacucc     1620

gaguacacgg ugcugaacga gaucaacaac cuccagauca ucaucgacgg caagaacauc     1680

ugcgacauca agcugaagca gaagaucuac gaggaccugu ucaagaagua caagaagauc     1740

acccagaagc agaucagcac cuucaucaag cacgagggga ucugcaacaa gaccgacgag     1800

gucaucaucc ucggcaucga caaggagugc acguccagcc ucaagagcua caucgagcug     1860

aagaacaucu ucgggaagca ggucgacgag aucuccacca agaacaugcu cgaggagauc     1920

auccgguggg cgaccaucua cgacgagggc gagggcaaga ccauccugaa gacgaagauc     1980

aaggccgagu acgggaagua cugcagcgac gagcagauca agaagauccu gaaccucaag     2040

uucuccggcu gggggcgccu gagccggaag uuccuggaga ccgugaccuc cgagaugccc     2100

ggcuucagcg agcccgugaa caucaucacc gccaugcgcg agacgcagaa caaccucaug     2160

gagcugcugu ccagcgaguu caccuucacc gagaacauca agaagaucaa cuccggguuc     2220

gaggacgccg agaagcaguu cagcuacgac ggccucguga agccgcuguu ccuguccccc     2280

agcgucaaga agaugcucug gcagacccug aagcugguga aggagaucuc ccacaucacc     2340

caggccccgc ccaagaagau cuucaucgag auggcgaagg gcgccgagcu cgagcccgcc     2400

cgcacgaaga cccggcugaa gauccugcag gaccucuaca acaacugcaa gaacgacgcc     2460

gacgcguuca gcuccgagau caaggaccug agcgggaaga ucgagaacga ggacaaccug     2520

cgccuccggu ccgacaagcu guaccuguac uacacccagc ucggcaagug cauguacugc     2580

gggaagccca ucgagaucgg ccacguguuc gacaccagca acuacgacau cgaccacauc     2640

uacccccaga gcaagaucaa ggacgacucc aucagcaacc gcguccuggu gugcuccagc     2700

ugcaacaaga acaaggagga caaguacccc cugaaguccg agauccagag caagcagcgg     2760

ggguucugga acuuccucca gcgcaacaac uucaucuccc uggagaagcu gaaccggcuc     2820

acgcgcgcca ccccgaucag cgacgacgag accgccaagu ucaucgcccg gcagcuggug     2880

gagacccgcc aggccacgaa ggucgcggcc aaggugcugg agaagauguu ccccgagacc     2940

aagaucgugu acuccaaggc cgagaccguc agcauguucc ggaacaaguu cgacaucgug     3000

aagugccgcg agaucaacga cuuccaccac gcccacgacg ccuaccucaa caucgugguc     3060

ggcaacgugu acaacaccaa guucacgaac aaccccugga acuucaucaa ggagaagcgg     3120

gacaacccca agaucgcgga caccuacaac uacuacaagg uguucgacua cgacgucaag     3180

cgcaacaaca ucaccgccug ggagaagggc aagaccauca ucacggugaa ggacaugcug     3240

aagcggaaca ccccgaucua cacccgccag gccgccugca agaaggggga gcuguucaac     3300

cagaccauca ugaagaaggg ccucgggcag cacccccuga agaaggaggg ccccuucucc     3360

aacaucagca aguacggcgg guacaacaag guguccgccg cguacuacac gcugaucgag     3420

uacgaggaga agggcaacaa gauccggagc cucgagacca ucccgcugua ccuggucaag     3480

gacauccaga aggaccagga cgugcucaag uccuaccuga ccgaccugcu cgggaagaag     3540

gaguucaaga uccuggugcc caagaucaag aucaacagcc ugcucaagau caacggcuuc     3600

cccugccaca ucaccgggaa gacgaacgac uccuuccugc ugcgccccgc cguccaguuc     3660

ugcugcagca acaacgaggu gcucuacuuc aagaagauca uccgguucuc cgagauccgc     3720

agccagcgcg agaagaucgg caagaccauc agcccguacg aggaccuguc cuuccggagc     3780

uacaucaagg agaaccugug gaagaagacc aagaacgacg agaucggcga gaaggaguuc     3840

uacgaccucc ugcagaagaa gaaccuggag aucuacgaca ugcuccugac caagcacaag     3900

gacacgaucu acaagaagcg ccccaacucc gccaccaucg acauccuggu gaaggggaag     3960

gagaaguuca agagccucau caucgagaac caguucgagg ucauccugga gauccugaag     4020

cuguucuccg ccacccggaa cgugagcgac cugcagcaca ucggcggguc caaguacagc     4080

ggcguggcga agaucgggaa caagaucucc agccuggaca acugcauccu caucuaccag     4140

uccaucaccg gcaucuucga gaagcgcauc gaccugcuga aggucuga                  4188


<210>  2104
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2104
augaacgcca accgcgagau ggccuacuac aucggcaucg acauggggac cggcagcgug       60

gggauggcga ucaccgacga gaacuacaac cugcucaagg ucaagggcca ggacuacugg      120

uucgugcggg aguucgagac ggccaacacc cagaucaagc gccggaugca ccgcaucucc      180

aagcggcgcc ugcggcgcca ccaggugcgg gucgcccuga uccgcagcua cuucgccgag      240

gagauccuca agaacgaccc ccuguucuuc auccggcagg acaacuccau cuacuaccug      300

gaggacaagg acagcgugcu caagaccaag gacugccugu ucgccgacua caacuaccac      360

gacaagaacu acuacaagga cuacccgacc aucuuccacc uccgccaggc guucaucaag      420

gacacgguga aggacgacga gaaccacacc cgguacgccc gccuccugua ccuggccauc      480

aucaacaugu ucgagcaccg ggggcacuuc cuccugaacu ccgucagcga ggacaucaac      540

aaggagaugg ugaaggacgc cggcgagggc uucacccugu accuccagga gcgcaucaag      600

gagaucaagc ccguguccua cagcgacauc cuggacgucc ugguggagaa gaacaucucc      660

cggagcgaga agaaggagcg caucguggag cuguucggga ucaagaaguc cugcaagaac      720

ggcauggaga ucaucaagug ccugugcggg cuggaggcga acgucgccau cauguucaac      780

cucgacgaca ucgagaacaa gcugaccauc guggugagca aggacgacua cgaggacaag      840

cuggccgagg uccagccccu cguggacgag gaguccuucg agcugcugag ccggaucaag      900

cagcucuacg acuacuccca gcugcagagc auccugaagg gcuacaagga gaaggaggac      960

ggggaggaca aguaccucuc cgacgugcgc gucgagcugu acgagaagca caaggccgac     1020

cugaagcucc ugaagaaggu guacaagcgg gaccuggaca agaagggcaa gaucugcgag     1080

ggcaagaaca uccagcgcag cuccaaguac aacuucaugu uccgcagcgg gaaggacggc     1140

acguacagcg ccuacaccaa cucccucaac agcuucugcu ccaagaccau cgagaacaag     1200

uaccggcgca acaugaagaa ccgggaccgc gcgagccugu acgacaccau caagaaggac     1260

cuguccggga ucgagaacga cgacgugaag aagauccucc gggacaucga gacggagaag     1320

uuccugccca agcagaucac cggcgagaac ggcgucaucc cgaaccagau ccacgccaag     1380

gagcugaagg ccauccucaa gaacgccgag aagcaccucc ccuuccugac cgagaaggac     1440

gacagcgggc ugaccguguc cgagcgcauc aucagccugu uccgguacug cauccccuac     1500

uacaucggcc ccguggggaa ggacucccgc acgggcuggg ccagcaucaa ggaggagggg     1560

cagaucuucc cguggaacau cgagcagaag aucgacgucg acaagaccuc cgaggaguuc     1620

aucacccggc ugaucaagac cugcacguac cucagcaacg agaagguggu ccccaagcag     1680

ucccuggugu acgagcgcua cugcgugcug aacgagauca acaacaucaa gauccggggc     1740

aagcgcaucg acccccagcu gaagcaggag aucuucaagg agcucuacau cggcagcaag     1800

ugcgggaaga aggucacccg gaagaaccug ugccaguacc uggugaacaa gggcgcgauc     1860

gaggaggacu ccgagaucag cgggaucgac aaggaccuca acaccuacau guccagcuac     1920

ggcaagaugu accagaucuu cggggacaag aucaagcgcg acgacaucaa ggagguggcc     1980

gaggagauca ucuacuacgg caccaucuac ggcggggcca aggagauguu caagaagaag     2040

aucgagcagu acgucuccga ggacauccug gacgaggccc agaugaaccg gauccugggc     2100

uucaaguuca aggacugggc ccgccucagc aagaaguucc uguaccugga gggguacgac     2160

aagggcaacc agaaccagcu cacgacccug augcaggcgc ugugggacua caaccucaac     2220

accauggagc ugaugaacag ccccauguuc accuucaagg agacgcugga gaagcagaag     2280

aacaagaugg agaaggugcu cugcgacuuc gaguacgagg accuggacga guacuacuuc     2340

uccgccccgg ugaagcggau ggucuggcag accaucgccu gccugaagga gguggugcag     2400

aucauggggc acaaccccaa gcgcaucuuc aucgagaugc cccgggccga cgaggagaag     2460

ggcgagcagg gccgcaaggc cagccgcaag aaccggcucc ugucccugua cgaggagauc     2520

gaggacgagc gccccuggaa gaaggagauc gagaacgcgg ccgagagcgg gcggcucaac     2580

uccaagcgca uguaccugua cuaccugcag augggcaagg acgccuacac cggggaggac     2640

aucgacaucg aggaccucuu cgacaacagc aaguacgaca ucgaccacau cuacccgcgg     2700

ucccugaccg acgacaacag ccuggagaac aaccucgucc ugguguccgc cacgaucaac     2760

caggacguga agaagaacga cuaccccauc cccuacgaca agauccgcgg caacagcaag     2820

guccgggagc uguggcgcuu ccuccacgag aagaaguuca ugaacgacga gaaguacaac     2880

cggcugaugu ccgacgugcc ccugacccag gagcagcucg cgggcuacau cgcccgccag     2940

cugguggaga ccgcccaggg gaccaagggc aucgccgaca ucaucaagag cgucaucccg     3000

gacgccgaca ucaucuacuc caaggcgaag aacgugagcg acuuccggaa gcgcuacggg     3060

augcugaagu cccggcucac gaacgacuuc caccacgcca aggacgccua ccugaacgug     3120

gucgugggca acguguacuu caccaaguuc acccagaacc cccgcaagua caucaaggac     3180

gagaacgaca ucgagaacaa gaaguacaac uacaaccuga gcaagaugua cgacaaggac     3240

gucauccgga acgggaaggu ggccuggauc gcccagaacg gcaagaaggc gggcaccauc     3300

cagaccauca agaaggugau ggaccgcaac acgcccauca ucacccggca ggccuucgag     3360

cagcgcgggg agcucuacga ccugcagccc aucuccaagc acaaggccaa gaaggagaac     3420

uacgucccga ucaaggccac caacgagaag augaaggacg ugaccaagua cggcggguuc     3480

acgagccuga accccgccua cuucguguuc gucgaguacg gcagcgcgaa gaagcggcag     3540

cgcugcuucg ccguggugca cuccuacugg gccagcucca ucaagaacaa cgaggaccuc     3600

gucacccacc ugaaggaccg gggguacgag aaccccaucg ugaucgccga caagaucaag     3660

aagaacagcc ugaucaagua caacggcuac uuccucuaca ucgugggcau ggacgcgcgc     3720

aagcaggucg aguucuccaa cgccaccagc augugccuga acaacaacuu cauccaguac     3780

auccagaaga ucgagaaggc caacacccug aucgccgagg ccaagaagcg ccggcccuac     3840

aucgacgagu ccaucacggc ggagcgcaac aucgcccucu acaacgagcu gaagaagaag     3900

caccuggaca gcaucuucaa gaaccacccg aaggggaucg gcuccgugcu cgagagcggg     3960

gagggcgccu ucgugcagcu gucccuggag cagcagaucg ggauccucua cagcaucauc     4020

caguucucca gcuucaagaa gggcaccuuc ucccugaccc ugaucggcgg gcccaaggag     4080

gucggccgga uccgcaucag cgggaacaug cccaaggccc gggagcucaa gcugauccac     4140

uacuccauca ccggcaugua caagaaggag aucaaccugc ucgacuga                  4188


<210>  2105
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2105
auggagaccc aggccagcaa ccagcugauc uccagccacc ucaagggcua ccccaucaag       60

gacuacuucg uggggcugga caucggcacc uccagcgucg ggugggccgu gacgaacaag      120

gcguacgagc ugcucaaguu ccgcucccac aagauguggg gcagccggcu guucgacgag      180

ggggaguccg ccguggcccg ccggggcuuc cgcagcaugc ggcgccggcu ggagcgccgg      240

aagcuccgcc ugaagcugcu cgaggagcug uucgccgacg ccauggcgca ggucgacccg      300

accuucuuca ugcggcugcg cgaguccaag uaccacuacg aggacaagac caccggccac      360

agcuccaagc acauccucuu caucgacaag aacuacacgg accaggacua cuacaaggag      420

uaccccacca ucuaccaccu ccggguggcc cugaugaagu ccgggaccga cgacauccgc      480

aagcuguucc uggccgugca ccacauccug aaguaccggg gcaacuuccu cuacgagggg      540

gccaccuucg acagcaacgc gagcacgcug gacgacgucc ugaagcaggc ccucgagaac      600

aucaccuuca acugcuucga cuccagcgcc gccaucggcu ccaucgggca gauccugaug      660

gagcugggca agaccaagag cgacaaggcc aaggcgaucg agcgccucgu ggacaccuac      720

aucaucacgg acgaggugga caccuccggc aagacccaga aggagcaggu caaggaggac      780

aagaagcggc ucaagagcuu cgccaaccug gugcuggggc ugagcgccuc ccugaccgac      840

cucuucggca gcguggagga ccuggacgag gaccugaaga agcuccagau caucggggac      900

acguacgacg agcgccggga cgaccuggcc aaggucuggg gcgacgagau ccacaucauc      960

gacgacugca aguccgugua cgacgccauc auccugauga gcaucaagga gcccggccuc     1020

accaucuccg agagcaaggu gaaggcguuc aagaagcaca aggacgaccu ggccauccug     1080

aagucccucc ucaagagcga ccgcuccauc uacaacacca uguucaaggu cgacgagaag     1140

gggcugcaca acuacgugca cuacaucaag cagggccgga ccgaggagac gagcugcaac     1200

cgcgaggacu ucuacaagua caccaagaag guggucgagg ggcugcccga cuccaaggac     1260

aaggaguaca uccugagcga gaucgagcug cagacccucc ugccgcugca gcgcaucaag     1320

gacaacggcg ugauccccua ccagcuccac cuggaggagc ugaaggugau ccuggccaag     1380

ugcgggccca aguuccccuu ccucaacgag gucgccgacg gcuucuccgu ggccgagaag     1440

cugaucaaga ugcuggaguu ccggaucccg uacuacgugg gcccccucaa cacccaccac     1500

aacgucgaca acgggggcuu cgcgugggcc gugcgcaagg ccagcgggcg ggucacgccc     1560

uggaacuucg acgacaagau cgaccgcgag aaguccgccg ccgcguucau caagaaccug     1620

accaacaagu gcaccuaccu gcucggcgag gacgugcugc ccaagagcuc ccugcucuac     1680

agcgaguuca ugcugcugaa cgagcucaac aacgugcgga ucgacgggaa gccgcuggag     1740

aaggccguca aggagcaccu gaucgaggcc guguucaagc aggaccacaa gaagaugacc     1800

aagaaccgca ucgagcaguu ccucaaggac aacaacuaca uccccgagaa gcacaagccc     1860

gagaucacgg gccuggacgg cgagaucaag aacgaccugg ccuccuaccg ggacauggug     1920

cgcauccucg ggaacaacuu cgaccugagc auggcggagg agaucaucac cgacaucacc     1980

aucuucggcg agagcaagaa gaugcugcgg gagacccucc gcaacaaguu cuccagccgg     2040

cuggacgacg aggccaucaa gaagcugucc aagcuccgcu accgggacug ggggcgccug     2100

agccggaagc ugcucaacgu caucgagggc ugcgacaagg ccggggacgg cacgcccgag     2160

accaucauca uccugaugcg caacuucucc uacaaccuga uggagcuccu gggcgacaag     2220

uucagcuuca uggagcggau ccaggagauc aacgccaagc ugaucgaggg gcagaucgug     2280

aacccgcacg acaucaucga cgaccucgcc cugucccccg cggugaagcg cgccgucugg     2340

caggcccugc ggaucgugga cgagaucgug cacaucaaga agacccuccc cgcccgcauc     2400

uucgucgagg ugacccgcag caacaaggcc gagaagaaga agaaggacuc ccggcagaag     2460

cgccugagcg accuguacgc ggccaucaag aaggacgacg cccuccuguc cggccugacg     2520

aacgaggagu ucgggggccu caagagcggc cuggccaagu acgacgacgc cgcgcugcgg     2580

uccaagaagc ucuaccugua cuacacccag auggggcgcu gcgccuacac cggcgaggug     2640

aucgaccuga gccagcucaa caccgacaac uacgacaucg accacaucua cccccggucc     2700

cugaccaagg acgacagcuu cgacaaccug guccucugca agcgcacggc caacgcccag     2760

aaguccgaca ccuacccgau cagcaaggag auccagaagg cgcagaaggc cuucuggauc     2820

uuccugaagc agcaggggcu gaucagcgag cggaaguacg agcgccucac ccggaucacg     2880

ccccugaccg ccgacgaccu guccggcuuc aucgcccgcc agcucgugga gaccaaccag     2940

agcgugaagg ccgcgaccac ccugcugcgg cgccucuacc ccggggucga cgugguguuc     3000

gucaaggccg agaacgugac ggacuuccgg cacgacaacg acuucaucaa ggugcgcucc     3060

cugaaccacc accaccacgc caaggacgcc uaccugaaca ucgucguggg caacguguac     3120

cacgagcggu ucacccgcaa cuuccgggcc uucuucaaga agaacggcgc gaaccgcacc     3180

uacaaccucg ccaagauguu caacuacgac gucaagugca ccaacgccaa ggacgggaag     3240

gccugggccg ugaagacgag cauggacacc gugaagaaga ugauggacuc caacgacguc     3300

cgggugacca agcgcgugua cgagcagaag ggccagcuga gcgacaucaa ccugaccaag     3360

gcguccaagg ucaaggccga caucuaccuc cccaucaagg ccaacgaccc gaucaugagc     3420

cagaacauga aguacggggg cuacuccgac aucaagaacg ccuacuacac gaucaucgag     3480

uacagcgaca agaagaacaa cuccaucaag gcguucaucc ccauccccau cuucaucgcc     3540

accaagaccc ugaccaagca ggcccugcuc gaguacauca agacggugcu gcccaaggcc     3600

aacaaccugc ggaucaucua caagaaggug uacaacgacc agcuccugaa gaucgacggg     3660

uucaacuacu accugggcgg ccgcagcaac gaccgcuucu gcaucgacaa cgccauccag     3720

cucaucgucc cgcuggagau ccaguucuac aucaagcugg uggagaaguu caccgcgauc     3780

aucuccgaga acaagaacau cgcccucaag gccagcuccg ucaagagccg gaaccccauc     3840

aacaaccagg uggagaacau cacccuggag uacacccaga agcuguucga cuaccucgug     3900

uccaaguuca acgagcugau cuaccugaag augcgcggga acaagcucac ggacgugaac     3960

aacaucggcc ggaagcaguu caucaagcug agccuggagg agcgcugcaa ggcccucgcc     4020

gagcugcuga acaucuucac caacaugaac aagaagcucu cccugagcac caucaacguc     4080

agcgcguccc ggaagguggu gaacuucaag aucagcuccc uggacgaguu caagaucguc     4140

aacgagagca ucaccgggcu cuacucccac gagaucacga ucguguga                  4188


<210>  2106
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2106
augaaccugc gcaacaccac cgacgaguac aacaucggcc ucgacauggg gacgaacagc       60

gugggcuggg ccgucaccga caaggugggg gccaucgcgc acuucaaggg caugcccacc      120

cugggguccc ggcuguucga cgccgcccag accgccgccg aggcgcgcgc ccaccggggc      180

cagcgccggc gcuacgugcg gcgccggugg cgccucgacc ugcugcagag ccucuuccag      240

gacgagaucg acaaggucga cuccgaguuc uucauccggc ugcgccagag ccggcugcac      300

guggaggacc gcgaggacgg ccacgccgac uacaaguggc cgaucuucaa cgacuccgac      360

uucacggagg uggacuacua caagcgguuc cccaccaucu accaccuccg caaguggcug      420

aucgagaccg accagaaggc cgacauccgg cugaucuacc ucgcggucca caacaucgug      480

aagcaccgcg ggaacuuccu gcgggagaac aacaagaacc ugagcucccg caacgccgac      540

cccgccgagg ccgugggcca ccuccggcug gcccugaaca acuggugccu cgagcgcggg      600

uacgagugcg cguggaaguc cggcgggaac gacaacgcca uccugaacgu ccugaucgac      660

ggcgagaagc gccggagcga ccaggugcgc gagaucgcca agcucaccgg caucgacugc      720

ggggacgcca agcagagccg gcagugcaac gaggccaucg cgaaggccau cgugggccug      780

aaggccgaga ugaagaacau cuucggggag uuccccgccg agaagacgcu gaucuaccuc      840

uccagcgacg acgacgucga gguggugcgc gacgccugcc cggacgacuc cgucgagcug      900

uuccaggcgc ugugcgagcu cuacgccgcc uacauccugc agggccugcu cagcuacgcc      960

cccggccaga ccaucuccgu gaacauggug aagaaguacg accgguacgg ggaggaccug     1020

caccuccuga aggagcugac ccgcaccuac gccagcaagc gguacgacgc guucuuccgc     1080

ggcgagacgu acgagggguc cagcgacuac gucguggaca aggcccaggg cuacacccgg     1140

uacaaccugg ggaccaccca gcucuccuac gacgacuucc agaaggccgu gaaggagcug     1200

uucaagggca cggacgcccu gcaggacgag cgcuaccagg gcaugauggu cgcguucggg     1260

gagcagcggu uccuccgccg gcugaagacc agcgacaacg gcagcaucua cuaccagcug     1320

caccucgagg agcugcacgc cauccuggag aaccagggga agcacuaccc cuuccuccug     1380

gccgaggagg acaagaucga gucccuggug agcuuccgca uccccuacua cgugggcccg     1440

cucacccggg agaacgccgc cgucgggcac gacggcaagg agcgcuucgc gugguccagc     1500

cggcgcgacg gcauggagca cgccgugauc acccccugga acugggagga gaucaucgac     1560

cgggacaagu ccgccgagga cuucaucauc cgcaugacgg gggacugcac cuaccugcag     1620

ggcgagcccg uccugcccaa gugcagccug cucuacgagg aguucugcgc ccugaacgag     1680

cugaacgggg cccacuggac caucgacgag gacgacgagc gccgguucga cgcggcccag     1740

cgcgagcgga ucaugugcga ccucuuccgc uccacccgga aggugacgua caagaaggug     1800

agcgacuggc uggagcgcga ggacuccuuc gucggcgccc gggugcacgg gggccagggc     1860

gagcacgggu ucgagagcca gcugggcucc uacaucuucu ucugcaagga cguguucaag     1920

accgacagcc ucgacgaggc cgacuacccg augaucgagc agaucauccu gugguccacc     1980

cuguucgagg accgcgacau ccuccggcgc aagaucgagg aggccuacgg ggaccggcug     2040

accacggagc agaucaaggu caucugcaag aagcgcuucg cgggcugggg ccggcugagc     2100

aagaagcucc ugaccgggau caagguggac accgacaccg gccgcaaguc caucaucgac     2160

gugcugcggg aggggaaccc caacgacggc cagcgcagcc ggacgauggu cuucauggag     2220

gugcuccgcg acgaggggcu gggcuuccag aaggccgugg acgacuucaa ccgggacuac     2280

uucuccgagc acaacaacgg ccugauggac gucaacgacc uccccgggag ccccgccauc     2340

cgccggucca ucaaccaggc cgugcgcauc guggacgaga ucgucagcau cgcgaagaag     2400

gagccggcca acaucuucau cgaggugacc cgcgaggagg acgacccccg gaagaagggc     2460

aagcgcacca agcggcgcua cgacgccauc aagagcgccc uggagguguu caagaaggac     2520

gacccccagc uguggaagga gcucaccaag aaggacucca acaacaugga cgagcggcug     2580

agccuguacu ucaugcagcg cgggaagugc auguacuccg gccggcccau caagaucgag     2640

gagcuccaca cggggaagua cgaggucgac cacaucaucc cgcgcagcua caucaaggac     2700

gacucccugg agaacaaggu gcugguguac cgggaggaga accagcacaa gaccgacacc     2760

cuccugaucg acagcuccau ccgccggaag augggcaagu acuggcgcau gcugcacaac     2820

gccaagcuca ucggcgacaa gaaguuccgg aaccuguucc gcgaccacau cgacgacaag     2880

accaugaagg gguucgucgc gcggcagauc guggagacca gccagauggu gaagcugguc     2940

cagacgcucc uggaggcccg cuaccccggc gcccggaucg ugcccgugaa ggccaacauc     3000

ucccacgacc ugcgcgacgc cgcggggcuc gucaagugcc gggaggccaa cgacuuccac     3060

cacgcccacg acgccuuccu ggccugccgc gugggccugu ucauccagaa gcgccacccc     3120

agcguguacg acaacccgau cgggcucgcg cacaucaugc gggacuacgc ccgcgcccag     3180

gcccgggagu acaagaaguc ccacuaccug gcgcacacca agggcuucau cgucaacagc     3240

uuccugucca gcggcuucga caaggagacc ggggaggugu ucaaggacga cugggacgcc     3300

gaggccgagg uggagggcau ccgccggguc cucaacuacc gccagugcuu caucucccgg     3360

augcccgugg aggccaccgg gacguucugg gaccagacca ucuacagccc caagugcggc     3420

aagaagcuga cccugccccu caagcgcggg cuggacccgg accgguacgg cuccuacagc     3480

cgcgagcagu ucgccuacuu cuucaucuac gaggcgcggg accugaagca cgagcgccac     3540

auccuccagu ucgcccaggu gcccguccgg guggccgccc acaucgccaa ggaccccagc     3600

cagcuggagu ccuacgcggu gaagcuggcc ggccaggcca accucgccuu cguccgcauc     3660

gugcggcgcc ggauccugaa gaagcagcug cucgagcuga acggggaccg ccugaucauc     3720

accggcgugc gggagguccg caacgccacg gaguucgcgu ucagccucga cgaggagacc     3780

cugaucgagc ggugcgugca gaucgccaag gugggguccc ugaucggcgu cgugaacgac     3840

gaggacgagg agcagcucag cgccgaccug aacgagcugu ucuccagccu cgugaagcgc     3900

ggcccgaagc ugugcaaccg ccuguacgcc cagcucaagc uggacaagua ccuguccaag     3960

uucgacgagc ucacccugac cgacaaggcc aaggucgugc ugcagcucau cgcgaucacg     4020

aacgggagca ucaacauggc cgaccugucc gcccugggca gcuccaagua cgccgggugc     4080

augcagguca gcuacuucaa gcagcucaac gcgcccaucg gcgaggugaa caucaucgac     4140

caguccguga ccgggauguu cgagcggcgc acccacaucg gccuguga                  4188


<210>  2107
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2107
augaaggaga ucaaggacua caucaucggg cuggacaucg ggaccaacag cugcggcuac       60

guggucaccg acaagcagaa caacauccuc aagcugaagg ggaagacggc caucggcgcc      120

cgccuguuca aggaggggca ggcggccgcc gaccggcgcu ccuuccggac cacccgccgg      180

cgccucgccc ggcgccacug gcggcugggc cugcucgagg agaucuucga ccccgagaug      240

gagaagaucg acccgaacuu cuuccgccgg cugaaggaga gcgacuacuc ccccaaggac      300

agccgcaagc aguucaacgc caucguguuc aaguccauca aggcggacaa gcgguucuac      360

aagaaguacc ccaccaucua ccaccuccgc aacgcccuca ugcacgacaa ccagaagcac      420

gaccugcggg agaucuucau ggccgugcac cacaucguca aguaccgcgg caacuuccug      480

cgggaggaca gcgugaacgc cuucaaggcg gccaaguucu cccuccgcgg ggaggacggc      540

aucgggcccg uggacaagcu gaacgaccug cucaaggaga ucuacagcga guacuccccg      600

gagcuggcca cgaacaaccu gagcaagauc gaggacauca ucaaggacaa gaaccucuac      660

aagcaggaca agcugaagca ggucgccacc cugcucauca aggaggccga cuccaaggac      720

aaggcgaagc ugaacaagga caucgccaag caggucgcca acagcuucau gggcuacaug      780

uuccggcugg acacccucuu cucccugacc gacguggacg ugaaggacua caagcugaag      840

uucagcgacg ccaacaucga cgagucccuc gacgaccuga cgagccugcu caccgacgcc      900

cagaucgagu ucgugcugga gcugcagucc aucuacaaca ccaucgugcu caacgagauc      960

guccccgacg ggaugagccu guccgagagc augaucaaga aguacgacga ccacaaggag     1020

gaccugaaga ucuucaagga guacaucgac agccucuccg accagaagaa ggcgaagaag     1080

cugcugagcg ccuacaaccu cuacgugaac uaccgcaagu ccgaccuggu ggaggccaag     1140

aaccuguuca agaacaagaa ggucggcgac aagaacuucg ccgacgugau cagcaacuuc     1200

gagguguucg gcaaguucgu cuccgacaac cucgacgaca gcgagcuggc caacaagauc     1260

aaggcgcggc uggaccucgg ggaguuccug cccaagcagc gcaccaacca gaacggcgug     1320

auccccuacc agcugcacca gguggagcuc acgcagaucc uggagaagca ggggaaguac     1380

uacccguucc ugaucacccc caaccccguc gagucccacc gcaacaacgc ccccuacgag     1440

aucagcgagc ugguguccuu ccgggugccg uacuacgucg gcccccucau cgacaaccag     1500

agcaucaagg acaagcagaa caagaacaag uucgccugga uggugcgcca gaagcagggc     1560

cagaucaccc ccuggaaccu ggaggagaug gucgacacca cggaguccgc caaccaguuc     1620

aucaagcgga ugacccgcaa ggacaccuac cugcucgccg aggacgugcu gcccaagagc     1680

ucccuggugu accagaaguu caugauccuc gacgagcuga accggaucaa gaucgacggg     1740

aagaagcuga ccagcgagcu caagcacgac aucuucgaga agcuguucaa gaagcagaag     1800

uccaucaacc uggacaaccu caagaacuac cugcuggucg agggcaacau cccggggcuc     1860

aucgagggcc ugagcgacgg gaucaacuuc aacaacuccu ucagcacgua caucgacuac     1920

cgcaacaucu ucggcgacga gaucgacaac cccaacaagc aggcggacuu cgagaagaug     1980

aucgaguggu ccaccguguu cgaggaccgg aagaucuuca agcgcaagcu gaaggagauc     2040

accuggcuca ccagcgagca gaucaagcag gucagcucca agcgguacag cggcuggggg     2100

cgccugucca agaagcugcu cacgcagauc accgacgaga acggcgugaa cauccugcag     2160

cggcugugga acgagcccga gacccucacc gaggugcugg ccaaccccgu gaucaagcgc     2220

aagaucgucg aggccaacag cuccuucacg aaggccaagg acaugaagga cauccuggug     2280

gaggcguaca ccagcccgca gaacaagaag gccauccgcc aggugaugcg ggucguggac     2340

gacaucaucg ccgccgccca cgggaagaag cccucccaga ucgcgaucga guucgcccgg     2400

gaggaccagg acaaccccca gaugaccaag agccgcaagg cccagcucga ccagaucuac     2460

gccaagaucu ccgacgaguu ccuggacgac gccgugaaga acgagcugaa gaacaugaag     2520

gacaacaagc cccucgcgaa ggacaagguc uuccuguacu ucaagcagau gggccgggac     2580

gccuacagcg gggagaagcu gucccucgac aaccugcagg acuacgacau cgaccacauc     2640

uacccgcaga gcuucaucaa ggacgacucc uucgacaacc gcgugcuggu gcagcgggcc     2700

uacaacaacg gcaagagcga caacgucccc gugcuccugu ucggcaacaa gaucgccgcc     2760

gggcugggca ucaccauccg ccagaugugg aagaaguggc ucgagcuggg gcugaucucc     2820

aagcgcaagu ucaacaaccu caucacggac cccgagaaga ucagccggaa cauggcgucc     2880

ggcuucguga accgccagcu ggucgagacc agccagguga ucaagcuggu ggccaacauc     2940

cuccaggcca agcugcccga caccgagauc aucgagguca aggccagcua caacuccauc     3000

cugcggaagg aguucaaccu cuacaagagc cgcgagguga acgacuacca ccacgcgauc     3060

gacgccuacc ugaccacgau cguggggaac uaccuguacc aggucuaccc gaagcuccgg     3120

cccuacuucg uguacggcca guucaagaag uucggcaagg ccgagaacca ggaggacgac     3180

cccgugaaga agaucaagaa guucaacuuc aucuaccagc ugaucugggg gaaggacgac     3240

gccaucugca ucucccacac caaccagcag gucuucagca agaaggacau caucgacaag     3300

cugaccaccg ccuacaacuu caaguacaug aacaucuccc acgcggugua cacccgcaac     3360

aacaacaugu ucaagcagac gcuguucccg aucaucgagc ggaccaccgc caagaccaag     3420

agccugaucc ccaagaagcu ggaccgcccc acggacaucu acggcgggua cuccaacaac     3480

aacgacgccu accucgcccu ggugaagguc gacaagaagc ggggcagcga guaccgcgug     3540

gugggcaucc ccaugcgggc cgcggccaug cugaaggccu ccaagaacua cgacgaggac     3600

cuccgcaagg ucaucgagcc gauggugaug uucgacaaga acgggaaggc caagcggggc     3660

aucgaggggu uccgcgugcu ggucggcaag augcccuuca agcaggccgu gcuggacggg     3720

aacaagaagu ucaugcucac cggcaccgug gacaccccca accugaagca gcugguccuc     3780

agccagcaga acaugaagua cauccuggac uacaucgagg acguggacug ccggaagcac     3840

aaguucggcg acaaggugca gaaccccgac aagugccugc ucgaggucuu cgacgagauc     3900

aucgagaucg uggacaagua cuucgagcug uucgacuccc gcgacuuccg ggagaagcug     3960

cgcaacagcc ggaacaaguu caucaagcuc gaggcgucca agaaggccga cacgauccug     4020

gccauccuga aggggcucca ggccaagagc acccgcgcga ccaucaagga gcugaacauc     4080

uccgacuucg gccggaucau caccagcucc aucagcccgg aggcccagcu gaucuaccag     4140

ucccccacgg gccucuucga gcgccgcgug aaggucaagg accuguga                  4188


<210>  2108
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2108
augagcaaga aguaccagaa caccaaguac uacgugggcc uggacaucgg gaccuccagc       60

gucggcuggg ccgugacgga cgccaacuac gagcuccuga aggugaacgg gcacaagacc      120

uggggcuccc gccuguucac cgaggcggag accgcccucg agcggcgcac gcaccgggcc      180

acccgccggc gccugcagcg gcgcaagcag cggcuggacc uccuggacga gcuguucgcc      240

gaggagcucg ccaaggucga cccccaguuc cugcugcgcc ucaaggagag ccaguaccac      300

auggaggaca aguccaccga cgacaaguac gugcuguuca acgaccagaa ccggaccgac      360

ggggaguucu ucacggagua cccgaccauc uaccaccucc gcagcgagcu caugcagggc      420

aagaaggagg acauccgggc gguguaccug gcccugcacc acaucgucaa guaccgcggc      480

cacuuccucc ugcccgggga caacuacgac augcagggca accuguccga gcucaucaag      540

cggcuguuca aguucaucga ggagacccug uucaucgagg accucgggcu gaccgacgag      600

auccagagca agaucgagga gauccugcuc acgaagacca agacccgccu ggaccggcag      660

aaggagaucg ugaagcuguu caacaccaag caccagaagc ucgacgacaa gcagaagaag      720

cugcuggagg ccaucuucaa gcucugccug ggccugaagg ggaacauggu gaacaucuuc      780

ggcgacaagg gcgaguacgg gaucgacuuc acggacgucg aggacaagaa gaagaaggug      840

gaguucuccg ccgacuacga caacaacauc aacgacuacg uggacuucuu cggcgaguac      900

uucgagcucc uggagaucac caagaagauc aacgacaccg ucaucuucag cgagauccug      960

guggacggga agggcuucuc cgagagcaag gugaucuccu acgagaagca ccagcaggac     1020

cuccgcaagc ugaagacccu gcucaagagc cuggacaaga cgcugggcua caaguacggg     1080

gagggccucu accagaagau ccugcuggac gacgucaagu ccaacuacgu gaacuacagc     1140

gggaacuccc agaagcucaa gaagugcacc aaggacgagu ucuacggcuu caucaagagc     1200

aagcugaacu ccgugaccaa gaaggagaag gucagcgagg cgcagcagaa ggagaucgcc     1260

cagauccugg ccgacaugga guucggggac uaccugcccc uccagcggac cacggacaac     1320

ggcgugaucc ccaaccagcu gcacaagaag gagcugaucg agaucaucga gcagcagacc     1380

aaguaccacg acuucuucaa cgacguggac gagaacggcc ucaccaucaa ggacaagauc     1440

aucagccuga ugaccuuccg caucccguac uacgucgggc cccugaacga cuaccacaag     1500

guggccgacg gcacggggca cgccuggauc gagaagaaga aggacggcaa gaucuacccc     1560

uggaacuucg acgagauggu ggacaccgag gcgaccgccg agaaguucau caagaaccuc     1620

accaacaagu gcacguaccu gaagggggag gacguccugc ccaaggaguc caagcucuac     1680

agcgaguuca ugcugcugaa cgagcucaac aacaucaaga ucgacaacca gaagcuggac     1740

ccgaagcuga agaugaagau gguguccgag cuguucauga agaagaacga gaagaucacc     1800

aagaagaaga ucgucgacua ccugcggagc aucggccagg ccaacggcaa cgagauggug     1860

uccgggaucg accgcgagau caagagcgac cugaagacca uccacaagau ccagcacuac     1920

uaccaggacg ccucccucga gauggccgag aacaucaucc gcuggaacac caucuucaac     1980

gacagcacgu ccgacauccu gaaggacaag aucaccaagg cguacggcga cgugcugacc     2040

cccgggcaga ucaagggccu cagcaaccug gccugcaagg acugggggaa gcuguccgag     2100

aaguuccuca ccggcauccg gugcgacguc ggcgaguuca agaacaucag caucauccag     2160

gccaugcgcg acaccaacaa caaccugaug gagcugcucu ccaacaagua cgaguucaug     2220

gacaagaucc aggaguacaa caaggaguac uacggggacu ucacguucaa ggccgagccc     2280

agcaucaugg acgaccugua cgugggcccc gggccgaagc gggccaucug gcaggcggug     2340

cgcaucgucg aggagaucaa gcggaucaug ggccacgacc ccgagaagau cuuccuggag     2400

accacccgca ccaaccgggc ccccaaggug aagaacacga agcgcuacga cgcccucgag     2460

aagaaguacg aguccgugaa ggagcacgag uacgccgagg ucaagaagga guucgagauc     2520

gagaagaacg acagccugaa caucaagaag cuguuccucu acuucaccca gcugggccac     2580

ugcauguacu ccgacgagaa gauccccuac aacgagcugu ucaccaaccg guacgacauc     2640

gaccacaucu acccgcgcag caagacccgg gacaacucca uggacaaccu cgugcuggug     2700

aaggccgagu gcaaccgcga caagaaggac aacuacccca ucaagcugaa gacgaacauc     2760

cagcagaagu gccagguccg gugggcguac cuccgcgaca agaagcugau caccgaggag     2820

aaguacaacc ggcuggugcg caagacgccc cucaccgagg acgagcugag ccaguucauc     2880

gcccggcagc ugguguucac cagccagucc gucaaggcca ccgccgaggu guucaagcgc     2940

cucuacgagg acagcaagau cugcuacgug aaggcggaga acgucuccga cuuccgguac     3000

gggagcaccc gcaagauguc caaggacgac cagccccagc cguucaucaa gaaccgcgac     3060

gugaacgacu uccaccacgc caaggacgcc uaccugaaca ucguggucgg caacguguac     3120

gacgugaagu ucacccagaa ccccacgcgg uucaucaagg ccaacaagga cuacagccug     3180

aacuacaucu uccgcuaccc cgucaacaag aagggggaga acguguggga ccccgagcag     3240

uccaugaaga ucguggagaa gacccucaag agcaacgacg uccgggugac caagcuggug     3300

aagccgauca ccucccccau cagcaacgag acgcugcaca aggcccucaa gugcaagccc     3360

ggcuccuacc ugccccgcaa gagcuccaag gggaccccgc uggcggacgu caccaaguac     3420

ggcggcuacg agaagaucaa gaacgccuac uacagcaucu acaaguacac cuacaugaag     3480

aagaaggagu gcauggagga guuccuccug gugcccaucc ccauccaccu ggugaacaac     3540

gacgaggagc ucccgaagua caucaacgac aagaucgccg agcgggccaa gaagaagaag     3600

cuggagacgg ucgagugcau cgugaaggag cuguaccagg ggacccucgu gcgcaucaac     3660

ggcuacgacu accggaucgc cggguccagc ggcaagcagu ucgagcugca caacgggauc     3720

aaccugaucg ucgagcccug gguggagaag gugcucaagg agaccgacaa guucguccgc     3780

aaguccaaca gcaagcugga caagaagcag cagaaggaga acaccacgga gacccugauc     3840

gagaagcagc ucaacgacgu guucgaguac cugugccgga agcugaacca gggcaucuuc     3900

aagaacaaca agcacacccu ccccgcgaag cugcugggcg acagcaucca gaagcgcuuc     3960

gaggacgccu ccaucgagga cgaguucgag gugaucaagg agcucaucag cgccaucgcc     4020

aagaaccccc agaacuucaa gaccauccag aagguccugg acaucuccgg gagcuccauc     4080

ccgcgguuca accugaagcu cacgaagaac aacguggacg aguucaucau caagaacgag     4140

agcgugaccg gccuguucac ccacgagaag gucaucuucc gcaaguga                  4188


<210>  2109
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2109
augaagaagg agaucaagga cuacuuccug ggccucgacg uggggaccgg cagcgucggg       60

ugggccguga ccgacacgga cuacaagcug cugaaggcca accgcaagga ccucuggggc      120

augcggugcu ucgagaccgc ggagaccgcc gaggugcgcc ggcugcaccg cggggcccgg      180

cgccggaucg agcgccggaa gaagcgcauc aagcugcucc aggagcuguu cucccaggag      240

aucgccaaga ucgacgaggg cuucuuccag cggaugaagg agagcccccu guacgccgag      300

gacaagacca uccuccagga gaacacgcug uucaacgaca aggacuucac cgacaagacc      360

uaccacaagg cguacccgac caucaaccac cugaucaagg ccuggaucga gaacaagguc      420

aagcccgacc cccgccuccu guaccuggcc ugccacaaca ucaucaagaa gcggggccac      480

uuccucuucg agggggacuu cgacuccgag aaccaguucg acacgagcau ccaggcccug      540

uucgaguacc ugcgcgagga cauggagcuc gacaucgacg cggacuccca gaaggugaag      600

gagauccuga aggacagcuc ccugaagaac agcgagaagc agucccggcu caacaagaug      660

cugggccuga agcccagcga caagcagaag aaggccauca ccaaccucau cuccgggaac      720

aagaucaacu ucgccgaccu guacgacaac ccggaccuga aggacgccga gaagaacagc      780

aucuccuuca gcaaggacga cuucgacgcc cucuccgacg accuggcgag cauccugggc      840

gacuccuucg agcuccugcu gaaggccaag gccguguaca acugcagcgu ccucuccaag      900

gugaucgggg acgagcagua ccugagcuuc gccaagguga agaucuacga gaagcacaag      960

accgaccuga ccaagcucaa gaacgucauc aagaagcacu uccccaagga cuacaagaag     1020

guguucggcu acaacaagaa cgagaagaac aacaacaacu acagcgccua cgugggcguc     1080

ugcaagauca cgcacaagaa gcuggugauc aagaacuccg cgagccagga ggacuucuac     1140

aaguuccuga agaccauccu cuccgccaag agcgagauca aggaggugaa cgacauccug     1200

accgagaucg agaccgggac guuccugccc aagcagaucu ccaagagcaa cgccgagauc     1260

cccuaccagc uccgcaagau ggagcuggag aagauccugu ccaacgccga gaagcacuuc     1320

agcuuccuca agcagaagga cgagaagggc cugucccaca gcgagaagau caucaugcug     1380

cucaccuuca agaucccgua cuacaucggg cccaucaacg acaaccacaa gaaguccuuc     1440

cccgaccggu gcugggucgu gaagaaggag aaguccccca gcggcaagac caccccgugg     1500

aacuucuucg accacaucga caaggagaag acggccgagg aguucaucac cagccgcacc     1560

aacuucugca ccuaccugau cggcgagucc gugcugccca agagcucccu gcucuacagc     1620

gaguacacgg uccugaacga gaucaacaac cugcagauca ucaucgacgg gaagaacauc     1680

ugcgacauca agcucaagca gaagaucuac gaggagcugu ucaagaagua caagaagauc     1740

acccagaagc agaucuccac cuucaucaag cacgagggca ucugcaacaa gaccgacgag     1800

gugaucaucc uggggaucga caaggagugc acgagcagcc ucaaguccua caucgagcug     1860

aagaacaucu ucggcaagca ggucgacgag aucagcacca agaacaugcu ggaggagauc     1920

auccgguggg cgaccaucua cgacgagggg gagggcaaga ccauccucaa gacgaagauc     1980

aaggccgagu acggcaagua cugcuccgac gagcagauca agaagauccu gaaccugaag     2040

uucagcgggu ggggccgccu cucccggaag uuccuggaga ccgugaccag cgagaugccc     2100

ggguucuccg agcccgugaa caucaucacc gccaugcgcg agacgcagaa caaccugaug     2160

gagcuccuga gcuccgaguu caaguucacc gagaacauca agaagaucaa cagcggcuuc     2220

gaggacgccg agaagcaguu cuccuacaac gggcugguga agccccucuu ccugagcccc     2280

uccgucaaga agaugcugug gcagacccuc aagcugguga aggagaucag ccacaucacc     2340

caggccccgc ccaagaagau cuucaucgag auggcgaagg gcgccgagcu ggagccggcc     2400

cgcaccaaga cgcggcucaa gauccugcag gaccuguacu ccagcugcaa gaacgacgcc     2460

gacgcguucu ccagcgagau ccaguaccuc agcggcaaga ucgagaacga ggacaaccug     2520

cgccugcggu ccgacaagcu cuaccuguac uacacccagc uggggaagug cauguacugc     2580

ggcaagccca ucgagaucgg gcacgucuuc gacaccagca acuacgacau cgaccacauc     2640

uacccccagu ccaagaucaa ggacgacagc aucuccaacc gcgugcucgu gugcagcucc     2700

ugcaacaaga acaaggagga caaguacccc cugaaggccg agauccagag caagcagcgg     2760

ggcuucugga acuuccugca gcgcaacaac uucaucuccc ucgagaagcu gaaccggcug     2820

acccgcacga gcccgaucuc cgacgacgag accgccaagu ucaucgcccg gcagcucguc     2880

gagacccgcc aggccaccaa gguggcggcc aaggugcugg agaagauguu ccccgagacg     2940

aagaucgucu acagcaaggc cgagaccgug uccauguucc ggaacaaguu cgacaucauc     3000

aagugccgcg agaucaacga cuuccaccac gcccacgacg ccuaccugaa caucgugguc     3060

gggaacgugu acaacaccaa guucaccaac aaccccugga acuucaucaa ggagaagcgg     3120

gacaacccca agaucgcgga cacguacaac uacuacaagg uguucgacua cgacgucaag     3180

cgcaacaaca ucaccgccug ggagaagggc aagaccauca ucaccgugaa ggacaugcuc     3240

aagcggaaca cgccgaucua cacccgccag gccgccugca agaagggcga gcuguucaac     3300

cagaccauca ugaagaaggg gcugggccag cacccccuca agaaggaggg gccccugagc     3360

aacaucucca aguacggcgg cuacaacaag gugagcgccg cguacuacac ccugaucgag     3420

uacgaggaga agggguccaa gauccggagc cucgagacga ucccgcugua ccuggucaag     3480

gacauccaga agaacaagga cggccucaag uccuaccuga ccgaccagau cgggaagaag     3540

gagaucaaga uccugauccc caagaucaag aucaacagcc uccugaagau caacggcuuc     3600

cccugccaca ucaccgggaa gaugggcgag gccuucgugc ugcgccccgc cgugcaguuc     3660

ugcugcagca acgacgaggc ccucuacuuc aagaagauca uccgguucaa cgagauccgc     3720

aagcagcgcg agaagauggg caagaccauc uccccguacg aggaccugag cuuccggucc     3780

uacaucaagg acaaccugug caagaagacg aaggcggacg agaucgagga gaaggaguuc     3840

uacgaccucc ugcagaagaa gaaccuggag aucuacgaca ugcuccugac caagcacaag     3900

gacaccaucu acaagaagcg ccccaacagc gccaccaucg acauccuggu caaggggaag     3960

gagaaguuca agucccucau caucgagaac caguucgagg ugauccugga gauccugaag     4020

cuguucagcg ccacgcggaa cguguccgac cuccagcaca ucggcgggag caagaacgcc     4080

ggcaacgccc gcgucgggaa caagaucucc agccuggaca acugcauccu caucuaccag     4140

uccaucaccg gcaucuucga gaagcggauc gaccugcuga agaucuga                  4188


<210>  2110
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2110
augagcuuca agaagauccu gggccucgac gugggggucg ccuccaucgg cugggccgug       60

gugcgcgaga acgaggagaa ccccaacuuc aacaccauca ccaagcuggg gguccgggug      120

gugccgauca cgagcgacga gaagcugaac uucgagaagg gcaaguccau caccagcaac      180

gcggagcgca cccucgcccg guccagccgc cggaaccugc agcgcuuccg gcugcgccgg      240

aagaaccuca ucgccauccu gcugcagaac cagaucauca cccccacgac gccccucacc      300

gagaagggga aguucaccac cuucgagacc cugaagaucc gcgccaaggc caccaaggag      360

aagaucgagc uggaggagcu cgcgcggguc cugcugcaca ucaacaagaa gcgcggcuac      420

aaguccagcc ggaaggugaa gaacuccgag gacggcaagg ccaucgacgg gauggaggcc      480

gccaagaucc ucuacgagaa caagcugacc cccggccagu acacguacca gcugcucgag      540

aaggggagca acaagauccc ggacuucuac ugcuccgacc ugcagaucga gugggaggug      600

aucuggaacu uccagaagca guucuacccc aaccugcuca ccgaggacuu caagaaggag      660

cugaagggca ucgggcagcg cgcgaccgcc gcccuguucc ucaagaagua cggcaucuac      720

accgcccuga acaagggcaa gcgggaggag aagaagcugc aggccuacaa guggcgcagc      780

aaguccguca gcgagaagau ccccguggag gagcucgcgu acgugaucgc cgagaucaac      840

ucccacauca cgaacagcuc cggguaccug ggcaccauca gcgaccgguc caaggagcug      900

uacuucaacc agcagaccau cggggagaac cucuacgccc aggucgccaa gaacacccac      960

gccaagcuga agaaccaggu guuccugcgc caggacuaca gcgcggaguu cgagcagauc     1020

ugggagacgc aggccaaguu ccacccccag cucaccccgg agcugaagaa gaagauccgg     1080

gacagcauca ucuucuacca gcgcaagcug cggucccaga agcgccucau cagcaucugc     1140

gaguucgaga accgcgagau cgagaucauc aagaacggca agaagaagaa gaagaagauc     1200

ggccugaagg ccgcccccaa guccagcccc cuguuccagg aguucaagau cuggcagguc     1260

cucaacaaca ucgugaucaa gcaggagggg aaggaccggc ugcugaccug ggaggagaag     1320

gagcuccugu ucaccgagcu gaacaucaag ggcaacaucu ccgggcagaa ggcccucgcg     1380

cugcugaagc ucagccccaa ggacacgaag aucaacguga ccauccugga gggcaaccac     1440

acccaggagc agcuguaccg cguguaccug cggauccucg ggauggaggg cuacgacguc     1500

uucgaccugc ugaagauccc ggagaaccag gacgagauca agcccgccga cuccaagauc     1560

agcgccgccg agaucaagga gaccgugaag uccguguuca agacgugcag caucuccccc     1620

agcauccucg acuucgaccc ccugcugccg aaccccgagu ucgagaacca guccagcuac     1680

cagcucuggc accuccugua cuccuacgag ggcgacagcu cccccagcgg gaacgagaag     1740

cugcuggagc ugcucaagaa caaguucggc uucaagaagg agcacgcccu gcugcucgcg     1800

uccaucagcu acaccuacau cuacgggaac cugagcucca aggccaugcg caagaucuac     1860

cccuacagca aggagaacac cuacuccgag gccugcaugc uggccggcua ccggcacagc     1920

aagaacgcgc ucccgcgcga ggagcugguc aaguaccagc ugaaggaccg gcucgagauc     1980

cugcccaaga acucccugcg caaccccgug guggagaagg uccucaacca gcugaucaac     2040

cuggugaaca gccucaugga cgaguacggg gccuucgacg agauccggcu ggagauggcc     2100

cgcgagcuga agaagaacgc caaggagcgg gagaaguggu ccaagaacau cgccgacgcg     2160

accagccgca accagaagca cgccgagauc cucaagaagg acuucaagau cuccaacccc     2220

agcaagaacg accugauccg guaccgccug uaccucgagc ugaaggccaa cggcuacaac     2280

gaccuguaca cgaacaccua caucgacauc aacuaccucu ucacccaccg guacgacauc     2340

gaccacauca ucccgcaguc caagguccug gacgacagcu ucuccaacaa ggugcuggug     2400

ccccgccagg ccaaccucga gaaguccaac cugaccgccu ucgacuacau cagccaccag     2460

ggcaaggaga aggaggagca cuaccugaac gucaucacgg agcucuacaa gcgggggagc     2520

aucuccgaga ccaaguacaa gaaccugcag aagaagggca acgagaucgg ggagggcuuc     2580

aucaaccgcg accugaagaa cacccaguac aucgugcgga aggcgcgcga gauccucagc     2640

cugaucaccc ccaacguggu caccacgacc ggggagauca ccgccaagcu gcgcaaggac     2700

uggaaccuca ucaacagcau gaaggagcug aacauggaca aguuccggaa cgcccgccug     2760

accgagcucg uggagaacuc ccggggcgag aagaaggaga ucaucaucga cuggaacaag     2820

cgcaacgacc agcggcacca cgccaucgac gcccuggugg ucgcguucac gacccgcaac     2880

cacgugcagu accugaacua caugaacgcc cgggaggacg agagccacaa gcagcacucc     2940

cacauccuca gcaucgagaa gaagaucaag uacgacaagu uccacgagaa cggcucccgc     3000

cagagccggu ucaucuaccc caccuccaac uuccgcgagg aggccaagaa gaagcugaag     3060

gagauccugg ugagcuacaa gaccaagaac aaggucguga cggugaacau caacaagccg     3120

aagaagaagg gcggguacuc ccccaagcac gagcucacgc cccgggggcc gcugcacaag     3180

gagaccaucu acggcaugau ccgcauccug cacaccaagg agaucaaggu cagcuccaag     3240

cucaacuucg agaccguggu gcggauccag aacccccgcu accggaacgc ccugcuggag     3300

cgccucaagg acaacuucaa cgaccccaag aaggcguuca gcggcaccaa cucccugacc     3360

aagaaccccg ucuuccugac cgccaacagc acggagaccg ugccggagaa ggugaccauc     3420

cagugguucg agaaggucua caccauccgg aagcccauca accccacgaa cuucaagaac     3480

gagaagaaca ucgagaaggu gcucgacccc accauccgca cccugcugaa gaagcggcuc     3540

caggaguaca acgggaacgc caaggaggcc uucuccgacc uggagaagaa cccgaucugg     3600

cugaaccagg agaagggcau cugcaucaag accgugacga ucaccgggau cagcaacguc     3660

gagucccucc acaccaagaa ggaccacuuc ggcaaggagc ugcuggacau caacgggaac     3720

cccauccccg uggacuucgu gagcaccggc aacaaccacc acgucgccau cuacgaggac     3780

uccgagggca accuccagga gaagguggug agcuucuacg aggcggucga gcgcgugaac     3840

caggggcugc ccgugaucga cacgcuguac aaccucgacc ugggcuggaa guuccuguuc     3900

accaugaagc agaacgagau guuccucuuc ccggccgacg acuucgaccc caacgacauc     3960

gacaucuacg acgagaagaa ccugauccug aucagcaagc accucuuccg gguccagaag     4020

cuguccagca aggacuacug cuuccgccac caccuggaga ccuccaucaa cuucaacgac     4080

cccgcccuca aggggaucac cuggcgccgg gagagcccca acggccugcg caaccuggug     4140

aaggugcggc ucaaccaccu ggggaagauc guccacaucg gcgaguga                  4188


<210>  2111
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2111
augaagaagg agaucaagga cuacuuccug ggccucgacg uggggaccgg cagcgucggg       60

ugggccguga ccgacacgga cuacaagcug cugaaggcca accacaagga ccucuggggc      120

augcgcugcu ucgagaccgc ggagaccgcc gagaagcggc gccuguuccg cggggcccgg      180

cggcgcaucg agcggcgccg gaagcgcauc aagcugcucc aggagcuguu cucccaggag      240

aucgccaaga ucgacgagga cuucuuccag cggauggagg agagccccuu cuacgccgag      300

gacaagacca uccugcagga gaacacgcug uucaacgacc gcgacuucac cgacaagacc      360

uaccacaagg cguacccgac caucaaccac cugaucaagg ccuggaucga gaacaaggug      420

aagcccgacc cccggcugcu cuaccuggcc ugccacaaca ucaucaagaa gcgcggccac      480

uuccuguucg agggcgacuu cgacuccgag aaccaguucg acacgagcgu ccaggcccuc      540

uucgaguacc ugcgggagga cauggagcug gacaucgacg cggacuccaa gaagaucaag      600

gagauccuca aggacagcgc ccugaagaac uccgacaagc agaaccgccu gaacaagcuc      660

cuggggcuca agagcuccga caagcagaag aaggcccuca ccaaccugau cagcggcaac      720

aaggccaacu ucgccgaccu guacgacaac cccgaccuca aggacgcgga gaagaacucc      780

aucagcuucu ccaaggacga cuucgacgcc cugagcgacg agcuggccuc cgugcucggg      840

gacagcuucg agcugcugcu ccgggccaag gccguguaca acugcuccgu ccugagcaag      900

gugaucggcg acgagcagua ccuguccuuc gcgaagguga agaucuacga gaagcacaag      960

aacgaccuca ccaagcugaa ggaggucgug aagaaguacu ucccgaucga guaccgcaac     1020

aucuucgggu acaagcagag cgagaacaac aacugcaacu acagccggua cgugggcguc     1080

ugcaaguccg gcagcaagaa gaaggugauc aacaagaccg ccucccagga ggacuucuac     1140

aaguuccuga aggacauccu ccugaagcgc agcgacgugc ccgaggccaa cgggauccug     1200

acggagaucg agaccggcac cuuccucccc aagcagaucu ccaagaacaa cgccgagauc     1260

cccuaccagc ugcggaagau cgagcuggag aagauccuca ccaacgccga gaaguucuuc     1320

agcuuccuga agcagaagga cgagaacggg cugucccaca gcgagaagau caucaugcuc     1380

cugacguuca agaucccgua cuacaucggc cccaucaaca ccucccacaa gcaguacuuc     1440

cccgaccgca gcugggucau caagaaggag aacgcgcccc aggagaagau caccccgugg     1500

aacuucuucg accacaucga caaggagaag accgccgagg ccuucaucac gucccgcacc     1560

aacuucugca ccuaccugau cggcgagagc gugcugccca agaacucccu ccuguacagc     1620

gaguacaccg ugcugaacga gaucaacaac cuccagauca ucaucgacgg gaagaacauc     1680

ugcgacguca agcugaagca gaagaucuac gaggagcugu ucaagaagua caagaaggug     1740

acgcagaagc agaucuccac cuucaucaag cacgagggca ucugcaacaa gaccgacgag     1800

gugaucaucc ucgggaucga caaggagugc accagcuccc ucaagagcua caucgagcug     1860

aagaacaucu ucggcaagca ggucgacgag aucuccacga agaacaugcu cgaggacauc     1920

auccgguggg ccaccaucua cgacgagggg gagggcaaga ccauccugaa gaccaagauc     1980

aaggccgagu acgagaagua cuacagcgac gagcagauca agaagauccu gaaccucaag     2040

cugagcggcu gggggcgccu gucccggaag uuccucgaga gcaucacguc cgagaucccc     2100

ggcuucaucg agcccgugaa caucaucacc gcgaugcgcg agacccagaa caaccugaug     2160

gagcugcuca gcuccaaguu caaguucaac gagaacauca agaagaucaa cagcggguuc     2220

gaggacaccg acaagcgguu cuccuacaac agccugguga agccgcuguu ccucuccccc     2280

agcgucaaga agaugcugug gcagacgcug aagcucguga aggagaucuc ccacaucacc     2340

caggccccgc ccaagaagau cuucaucgag auggccaagg gcgccgagcu ggagcccgcg     2400

cgcaccaaga cccggcugaa gauccuccag gaccuguaca acagcugcaa gaacgacgcc     2460

gacgccuucu ccagcgagau ccaguaccug cucgggaaga ucgagaacga ggacaaccug     2520

cgccugcggu ccgacaagcu cuaccuguac uacacccagc ugggcaagug cauguacugc     2580

ggcaagccca ucgagaucgg gcacgucuuc gacacgagca aguacgacau cgaccacauc     2640

uacccccagu ccaagaucaa ggacgacagc aucaacaacc gcgugcucgu gugcagcucc     2700

ugcaacaaga acaaggagga caaguacccc cugaagccgg agauccagcu gaagcagaag     2760

aaguucugga acuuccucca gcggaacaac uucaucagca ucgagaaguu caaccgccug     2820

acccggacca cccccaucuc cgacgacgag acggccaagu ucaucgcccg ccagcugguc     2880

gagacccggc aggcgaccaa gguggccgcc aaggugcucg agaagaucuu ccccgagacc     2940

aacaucgucu acagcaaggc cgagacggug uccauguucc gcaacaaguu cgacaucauc     3000

aagugccggg agaucaacga cuuccaccac gcccacgacg cguaccugaa caucgugguc     3060

ggcaacgugu acaacaccaa guucaccaac aaccccugga acuucaucaa ggagaagcgc     3120

gacaacccga agaucgggga caccuacaac uacuacaagg uguucgacua cgacgucaag     3180

cggaacaaca ucacggccug ggagaaggag aagaccauca ucaccgugaa gaccaugcug     3240

aagcgcaaca cgcccaucua cacccgccag gccgccugca agaagggcga gcuguucaac     3300

cagaccauca ugaagaaggg gcugggccag cacccccuga agaaggagug cccguucagc     3360

aacaucucca aguacggcgg guacaacaag gugagcgccg cguacuacac cuucaucgag     3420

uacgaggaga agggcaacaa gauccggucc cucgagacga ucccccugua ccuggagaag     3480

gacauccaga agucccagga cgggcucaag ggcuaccuga ccgaccagcu gggcaagaag     3540

gagaucaaga uccucguccc caagaucaag aucaacagcc ugcugaagau caacggguuc     3600

cccugccaca ucaccggcaa gaccggggcc agcuucgugc uccgcccggc cgugcaguuc     3660

ugcugcucca acgacgaggu ccuguacuuc aagaagauca uccgguucaa cgagauccgc     3720

agccagcggg agaagauggg caagacgauc ucccccuacg aggaccugag cuuccgcucc     3780

uacaucaagg agaaccucug caagaagacc aagaacgacg agaucgggga gaaggaguuc     3840

uacggcuucc ugcagaagaa gaaccuggag aucuacgaca ugcuccugau caagcacaag     3900

gacaccaucu acagcaagcg gcccaacucc accacgcugg acaugcucau caagggccgc     3960

uacgaguuca ucaaccugaa gcccgaggac cagauccggg ugaugcugga gauccucaag     4020

cuguucugca ccacccgcga ggugaucgac cuggggcuca ucaagggcaa gagcgaggcc     4080

ggggucacca cgcugggcaa gaagaucagc aaccuggacu ccugcauccu caucuaccag     4140

agcaucaccg ggaucuucga gaagcggauc gaccugcuga agaucuga                  4188


<210>  2112
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2112
augaagaagg agaucaagga cuacuuccug ggccucgacg uggggaccgg cagcgucggg       60

ugggccguga ccgacacgga cuacaagcug cugaaggcca accgcaagga ccucuggggc      120

augcggugcu ucgagaccgc ggagaccgcc gaggugcgcc ggcugcaccg cggggcccgg      180

cgccggaucg agcgccggaa gaagcgcauc aagcugcucc aggagcuguu cucccaggag      240

aucgccaaga ccgacgaggg cuucuuccag cggaugaagg agagccccuu cuacgccgag      300

gacaagacga uccugcagga gaacgcccuc uucaacgacc gcgacuucac cgacaagacc      360

uaccacaagg ccuacccgac caucaaccac cugaucaagg ccuggaucga gaacaagguc      420

aagcccgacc cccggcugcu cuaccuggcc ugccacaaca ucaucaagaa gcgcggccac      480

uuccuguucg agggggacuu cgacuccgag aaccaguucg acacgagcau ccaggcccuc      540

uucgaguacc ugcgggagga cauggaggug gacaucgacg cggacuccca gaaggugaag      600

gagauccuga aggacagcuc ccucaagaac agcgagaagc agucccgccu gaacaagaug      660

cugggccuca agcccagcga caagcagaag aaggccauca ccaaccugau cuccgggaac      720

aagaucaacu ucgccgaccu guacgacaac ccggaccuca aggacgccga gaagaacagc      780

aucuccuuca gcaaggacga cuucgacgcg cuguccgacg accuggccag cauccucggc      840

gacuccuucg agcugcugcu caaggccaag gccgucuaca acugcagcgu gcuguccaag      900

gugaucgggg acgagcagua ccugagcuuc gccaagguca agaucuacga gaagcacaag      960

accgaccuca ccaagcugaa gaacgugauc aagaagcacu uccccaacga cuacaagaag     1020

guguucggcu acaacaagaa cgagaagaac aacaacaacu acagcgcgua cgucggcgug     1080

ugcaagacga aguccaagaa gcuggugauc aacaacaccg ucaaccagga ggacuucuac     1140

aaguuccuca agcccauccu gcuggccaag agcgagauca aggaggugaa cgacauccuc     1200

accgagaucg agaccgggac guuccugccc aagcagaucu ccaagagcaa cgccgagauc     1260

ccguaccagc ugcggaagau ggagcucgag aagauccugu ccaacgccga gaagcacuuc     1320

agcuuccuga agcagaagga cgagaagggc cucucccaca gcgagaagau caucaugcug     1380

cugaccuuca agauccccua cuacaucggg cccaucaacg acaaccacaa gaaguccuuc     1440

cccgaccgcu gcugggugau caagaaggag aaguccccga gcggcaagac cacccccugg     1500

aacuucuucg accacaucga caaggacaag acggccgagg cguucaucac cagccggacc     1560

aacuucugca ccuaccucgu cggcgagucc gugcugccca agaacagccu gcuguacucc     1620

gaguacacgg ugcucaacga gaucaacaac cugcagauca ucaucgacgg gaagaacauc     1680

ugcgacauca agcugaagca gaagaucuac gaggagcugu ucaagaagua caagaagauc     1740

acccagaagc agaucagcac cuucaucaag cacgagggca ucugcaacaa gaccgacgag     1800

gucaucaucc ucgggaucga caaggagugc acguccagcc ugaaguccua caucgagcuc     1860

aagaacaucu ucggcaagca ggucgacgag aucagcacca agaacaugcu ggaggagauc     1920

auccgcuggg ccaccaucua cgacgagggg gagggcaaga ccauccugaa gacgaagauc     1980

aaggccgagu acggcaagua cugcagcgac gagcagauca agaagauccu caaccugaag     2040

uucuccgggu ggggccggcu gagccgcaag uuccucgaga ccgugaccuc cgagaugccc     2100

ggguucagcg agccggugaa caucaucacc gccaugcgcg agacgcagaa caaccugaug     2160

gagcugcucu ccagcgaguu caaguucacc gagaacauca agaagaucaa cuccggcuuc     2220

gaggacgugg agaagcaguu cagcuacgac ucccugguca agccccuguu ccucagcccc     2280

uccgugaaga agaugcugug gcagacccug aagcucguga aggagaucag ccacaucacc     2340

caggcgcccc cgaagaagau cuucaucgag auggcgaagg gggccgagcu ggaccccguc     2400

cggaccaaga cgcgccugaa gauccuccag gaccuguaca acaacugcaa gaacgacgcc     2460

gacgccuucu ccagcgagau caaggaccug uccggcaaga ucgagaacga ggacaaccuc     2520

cggcugcgca gcgacaagcu guaccucuac uacacccagc ugggcaagug cauguacugc     2580

gggaagccca ucgagaucgg ccacguguuc gacaccucca acuacgacau cgaccacauc     2640

uacccccaga gcaagaucaa ggacgacagc aucuccaacc gggugcuggu cugcagcucc     2700

ugcaacaaga acaaggagga caaguacccg cucaaggcgg agauccagag caagcagcgc     2760

ggguucugga acuuccugca gcggaacaac uucaucuccc uggagaagcu caaccgccug     2820

acccggacga gccccaucuc cgacgacgag accgccaagu ucaucgcccg ccagcuggug     2880

gagacccggc aggccaccaa gguggccgcg aagguccucg agaagauguu ccccgagacg     2940

aagaucgugu acagcaaggc cgagaccgug uccauguucc gcaacaaguu cgacaucauc     3000

aagugccggg agaucaacga cuuccaccac gcccacgacg ccuaccugaa caucgucgug     3060

ggcaacgugu acaacaccaa guucaccaac aaccccugga acuucaucaa ggagaagcgc     3120

gacaacccga agaucgccga cacguacaac uacuacaagg ucuucgacua cgacgugaag     3180

cggaacaaca ucaccgcgug ggagaagggg aagaccauca ucaccgugaa ggacaugcug     3240

aagcgcaaca cgcccaucua cacccggcag gccgccugca agaagggcga gcuguucaac     3300

cagaccauca ugaagaaggg ccucgggcag cacccccuga agaaggaggg cccgcucagc     3360

aacaucucca aguacggggg cuacaacaag gucagcgccg ccuacuacac ccugaucgag     3420

uacgaggaga agggcuccaa gauccgcagc cuggagacga ucccccucua ccuggugaag     3480

gacauccaga agaacaagga cgggcugaag uccuaccuca ccgaccagau cggcaagaag     3540

gagaucaaga uccugauccc caagaucaag aucaacagcc ugcucaagau caacggguuc     3600

cccugccaca ucaccggcaa gaugggggag gcguucgugc ugcggccggc cguccaguuc     3660

ugcugcucca acgacgaggc ccuguacuuc aagaagauca uccgcuucaa cgagauccgc     3720

aagcagcggg agaagauggg caagaccauc agccccuacg aggaccucuc cuuccgcagc     3780

uacaucaagg acaaccugug caagaagacg aaggccgacg agaucgagga gaaggaguuc     3840

uacgaccugc uccagaagaa gaaccuggag aucuacgaca ugcugcucac caagcacaag     3900

gacaccaucu acaagaagcg gcccaacagc gcgaccaucg acauccuggu gaagggcaag     3960

gagaaguuca agucccugau caucgagaac caguucgagg ugauccucga gauccugaag     4020

cuguucagcg ccacgcgcaa cgucuccgac cuccagcaca ucgggggcag caagaacgcc     4080

gggaacgccc gggugggcaa caagaucucc agccuggaca acugcauccu gaucuaccag     4140

uccaucaccg ggaucuucga gaagcgcauc gaccuccuga agaucuga                  4188


<210>  2113
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2113
augaagaagg agaucaagga cuacuuccug ggccucgacg uggggaccgg cagcgucggg       60

ugggccguga ccgacacgga cuacaagcug cugaaggcca accgcaagga ccucuggggc      120

augcggugcu ucgagaccgc ggagaccgcc gaggugcgcc ggcugcaccg cggggcccgg      180

cgccggaucg agcgccggaa gaagcgcauc aagcugcucc aggagcuguu cucccaggag      240

aucgccaaga ucgacgaggg cuucuuccag cggaugaagg agagccccuu cuacgccgag      300

gacaagacca uccugcagga gaacgcccuc uucaacgaca aggacuucac ggacaagacc      360

uaccacaagg cguacccgac caucaaccac cugaucaagg ccuggaucga gaacaagguc      420

aagcccgacc cccgccugcu cuaccuggcc ugccacaaca ucaucaagaa gcggggccac      480

uuccuguucg agggggacuu cgacuccgag aaccaguucg acaccagcau ccaggcccuc      540

uucgaguacc ugcgcgagga cauggaggug gacaucgacg cggacuccca gaaggugaag      600

gagauccuga aggacagcuc ccucaagaac agcgagaagc agucccggcu gaacaagauc      660

cugggccuca agcccagcga caagcagaag aaggccauca cgaaccugau cuccgggaac      720

aagaucaacu ucgccgaccu guacgacaac ccggaccuca aggacgccga gaagaacagc      780

aucuccuuca gcaaggacga cuucgacgcg cuguccgacg accuggccag cauccucggc      840

gacuccuucg agcugcugcu caaggccaag gccgucuaca acugcagcgu gcuguccaag      900

gugaucgggg acgagcagua ccugagcuuc gccaagguca agaucuacga gaagcacaag      960

accgaccuca ccaagcugaa ggcggugauc aagaagcacu uccccaacaa cuacaagaag     1020

guguucggcu acaaccagga caacgagagc aagaacaacu acuccggcua cgucggggug     1080

ugcaagacca agaacaagaa gcuggugauc aacaacagcg ucaaccagga ggacuucuac     1140

aaguuccuca agcccauccu guccgccaag agcgagauca aggaggugaa cgacauccug     1200

gccgagaucg agacgggcaa cuuccucccc aagcagaucu ccaagagcaa cgccgagauc     1260

ccguaccagc ugcgcaagau ggagcuggag aagauccucu ccaacgccga gaagcacuuc     1320

agcuuccuga agcagaagga cgagaagggg cugucccaca gcgagaagau caucaugcuc     1380

cugaccuuca agauccccua cuacaucggc cccaucaacg acaaccacaa gaaguccuuc     1440

cccgaccggu gcuggguggu caagaaggag aaguccccga gcggcaagac cacccccugg     1500

aacuucuucg accacaucga caaggagaag acggcggagg aguucaucac cagccgcacc     1560

aacuucugca ccuaccugau cggggagucc gugcucccca agaacagccu gcuguacucc     1620

gaguacacgg ugcugaacga gaucaacaac cuccagauca ucaucgacgg caagaacauc     1680

ugcgacacca agcugaagca gaagaucuac gaggaccugu ucaagaagua caagaagauc     1740

acccagaagc agaucagcac cuucaucaag cacgagggga ucugcaacaa gacggacgag     1800

gucaucaucc ucggcaucga caaggagugc accuccagcc ucaagagcua caucgagcug     1860

aagaacaucu ucgggaagca ggucgacgag aucuccacca agaacaugcu cgaggagauc     1920

auccgguggg ccaccaucua cgacgagggc gagggcaaga cgauccugaa gaccaagauc     1980

aaggccgagu acgggaagua cugcagcgac gagcagauca agaagauccu gaaccucaag     2040

uucuccggcu gggggcgccu gagccggaag uuccuggaga ccgugaccuc cgagaugccc     2100

ggcuucagcg agccggugaa caucaucacg gccaugcgcg agacccagaa caaccucaug     2160

gagcugcugu ccagcgaguu caaguucacc gagaacauca agaagaucaa cuccggguuc     2220

gaggacgccg agaagcaguu cagcuacgac ggccucguga agccccuguu ccuguccccc     2280

agcgucaaga agaugcucug gcagacccug aagcugguga aggagaucuc ccacaucacg     2340

caggcgcccc cgaagaagau cuucaucgag auggccaagg gcgccgagcu cgagcccgcc     2400

cgcaccaaga cccggcugaa gauccugcag gaccucuaca gcuccugcaa gaacgacgcg     2460

gacgccuuca gcuccgagau ccaguaccug agcgggaaga ucgagaacga ggacaaccug     2520

cgccuccgga gcgacaagcu guaccuguac uacacccagc ucggcaagug cauguacugc     2580

gggaagccca ucgagaucgg ccacguguuc gacaccucca acuacgacau cgaccacauc     2640

uacccccaga gcaagaucaa ggacgacucc aucagcaacc gcguccuggu gugcuccagc     2700

ugcaacaaga acaaggagga caaguacccg cugaaguccg agauccagag caagcagcgg     2760

ggguucugga acuuccucca gcgcaacaac uucaucuccc uggagaagcu gaaccggcuc     2820

acgcgcacca cccccaucag cgacgacgag accgccaagu ucaucgcccg gcagcuggug     2880

gagacgcgcc aggccaccaa ggucgcggcc aaggugcugg agaagauguu ccccgagacc     2940

aagaucgugu acuccaaggc cgagaccguc agcauguucc ggaacaaguu cgacaucauc     3000

aagugccgcg agaucaacga cuuccaccac gcccacgacg ccuaccucaa caucguggug     3060

ggcaacgucu acaacacgaa guucaccaac aaccccugga acuucaucaa ggagaagcgg     3120

gacaacccga agaucgcgga caccuacaac uacuacaagg uguucgacua cgacgugaag     3180

cgcaacaaca ucaccgccug ggagaagggc aagacgauca ucaccgucaa ggacaugcug     3240

aagcggaaca cccccaucua cacccgccag gccgccugca agaaggggga gcuguucaac     3300

cagacgauca ugaagaaggg ccucgggcag cacccccuga agaaggaggg cccguucucc     3360

aacaucagca aguacggcgg guacaacaag guguccgccg cguacuacac ccugaucgag     3420

uacgaggaga aggugaacaa gauccggagc cucgagacca ucccccugua ccuggucaag     3480

gacauccaga aggaccagga cgugcucaag uccuaccuga ccgaccugcu cggcaagaag     3540

gagaucaaga uccuggugcc caagaucaag aucaacagcc ugcucaagau caacggguuc     3600

cccugccaca ucacgggcaa gaccggggac uccuucgucc ugcgcccggc cgugcaguuc     3660

ugcugcagca acgacgaggu gcuguacuuc aagaagauca uccgguucaa cgagauccgc     3720

agccagcgcg agaagauggg caagaccauc ucccccuacg aggaccucag cuuccggucc     3780

uacaucaagg agaaccugug caagaagacc aaggccgacg agaucggcga gaaggaguuc     3840

uacgaccugc uccagaagaa gaaccuggag aucuacgaca ugcugcucac gaagcacaag     3900

gacaccaucu acaacaagcg ccccaacagc accacccugg ggaugcugac gaagggccgg     3960

uacgaguuca ucaaccucaa gcccaaggac cagaucaucg ucaugcugga gauccugaag     4020

cuguucugca ccacccgcga ggccaucgac cuggggcuga ucaagggcaa gccggcggcc     4080

gggguggcca cccucggcaa gaagaucucc aaccuggaca acugcauccu gaucuaccag     4140

agcaucacgg gcaucuucga gaagcggauc gaccuccuga agguguga                  4188


<210>  2114
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2114
augaagaagg agaucaagga cuacuuccug ggccucgacg uggggaccgg cagcgucggg       60

ugggccguga ccgacacgga cuacaagcug cugaaggcca accgcaagga ccucuggggc      120

augcggugcu ucgagaccgc ggagaccgcc gaggugcgcc ggcugcaccg cggggcccgg      180

cgccggaucg agcgccggaa gaagcgcauc aagcugcucc aggagcuguu cucccaggag      240

aucgccaaga ucgacgaggg cuucuuccag cggaugaagg agagcccccu guacgccgag      300

gacaagacca uccuccagga gaacacgcug uucaacgaca aggacuucac cgacaagacc      360

uaccacaagg cguacccgac caucaaccac cugaucaagg ccuggaucga gaacaagguc      420

aagcccgacc cccgccuccu guaccuggcc ugccacaaca ucaucaagaa gcggggccac      480

uuccucuucg agggggacuu cgacuccgag aaccaguucg acacgagcau ccaggcccug      540

uucgaguacc ugcgcgagga cauggaggug gacaucgacg cggacuccca gaaggugaag      600

gagauccuca aggacagcuc ccugaagaac agcgagaagc agucccggcu gaacaagaug      660

cucggccuga agcccagcga caagcagaag aaggccauca ccaaccugau cuccgggaac      720

aagaucaacu ucgccgaccu cuacgacaac ccggaccuga aggacgccga gaagaacagc      780

aucuccuuca gcaaggacga cuucgacgcc cuguccgacg accucgcgag cauccugggc      840

gacuccuucg agcugcuccu gaaggccaag gccgucuaca acugcagcgu gcuguccaag      900

gugaucgggg acgagcagua ccucagcuuc gccaagguca agaucuacga gaagcacaag      960

accgaccuga ccaagcugaa gaacgugauc aagaagcacu uccccaagga cuacaagaag     1020

guguucggcu acaacaagaa cgagaagaac aacaacaacu acagcggcua cgucggggug     1080

ugcaagacga aguccaagaa gcucaucauc aacaacagcg ugaaccagga ggacuucuac     1140

aaguuccuga agaccauccu guccgccaag agcgagauca aggaggucaa cgacauccuc     1200

accgagaucg agaccggcac guuccugccc aagcagaucu ccaagagcaa cgcggagauc     1260

cccuaccagc ugcgcaagau ggagcucgag aagauccugu ccaacgccga gaagcacuuc     1320

agcuuccuga agcagaagga cgagaagggg cucucccaca gcgagaagau caucaugcug     1380

cugaccuuca agaucccgua cuacaucggc cccaucaacg acaaccacaa gaaguccuuc     1440

cccgaccggu gcuggguggu gaagaaggag aaguccccca gcggcaagac caccccgugg     1500

aacuucuucg accacaucga caaggagaag acggccgagg aguucaucac cagccgcacc     1560

aacuucugca ccuaccucau cggggagucc guccugccca agaacagccu gcuguacucc     1620

gaguacacgg ugcucaacga gaucaacaac cugcagauca ucaucgacgg caagaacauc     1680

ugcgacacca agcugaagca gaagaucuac gaggagcugu ucaagaagua caagaagauc     1740

acccagaagc agaucagcac cuucaucaag cacgagggga ucugcaacaa gacggacgag     1800

gugaucaucc ugggcaucga caaggagugc accuccagcc ucaagagcua caucgagcuc     1860

aagaacaucu ucgggaagca ggucgacgag aucuccacca agaacaugcu ggaggagauc     1920

auccgguggg ccaccaucua cgacgagggc gagggcaaga cgauccugaa gaccaagauc     1980

aaggccgagu acgggaagua cugcagcgac gagcagauca agaagauccu caaccugaag     2040

uucuccggcu gggggcgccu gagccggaag uuccucgaga ccgugaccuc cgagaugccc     2100

ggcuucagcg agcccgugaa caucaucacg gcgaugcgcg agacccagaa caaccugaug     2160

gagcugcucu ccagcgaguu caaguucacc gagaacauca agaagaucaa cuccggguuc     2220

gaggacgucg agaagcaguu cagcuacgac ggccugguga agccgcuguu ccucuccccc     2280

agcgucaaga agaugcugug gcagacccug aagcucguga aggagaucuc ccacaucacc     2340

caggccccgc ccaagaagau cuucaucgag auggccaagg gcgccgagcu ggaccccgug     2400

cgcacgaaga cccggcugaa gauccuccag gaccuguaca acaacugcaa gaacgacgcc     2460

gacgcguuca gcuccgagau caaggaccug agcgggaaga ucgagaacga ggacaaccuc     2520

cgccugcggu ccgacaagcu guaccucuac uacacccagc ugggcaagug cauguacugc     2580

gggaagccca ucgagaucgg ccacgucuuc gacaccagca acuacgacau cgaccacauc     2640

uacccccaga gcaagaucaa ggacgacucc aucagcaacc gcgugcuggu gugcuccagc     2700

ugcaacaaga acaaggagga caaguacccc cucaaggccg agauccaguc caagcagcgg     2760

ggguucugga acuuccugca gcgcaacaac uucaucagcc uggagaaguu caaccggcuc     2820

acgcgcgcca ccccgaucuc cgacgacgag accgccaagu ucaucgcgcg gcagcugguc     2880

gagacccgcc aggccacgaa gguggccgcc cgggugcugg agaagauguu ccccgagacc     2940

aagaucgucu acagcaaggc cgagaccgug uccauguucc gcaacaaguu cgacaucgug     3000

aagugccggg agaucaacga cuuccaccac gcgcacgacg ccuaccucaa caucgucgug     3060

ggcaacgugu acaacaccaa guucacgaac aaccugugga acuucaucaa ggagaagcgc     3120

gacaacccca agaucgccga caccuacaac uacuacaagg ucuucgacua cgacgugaag     3180

cggaacaaca ucaccgccug ggagaagggc aagaccauca ucacggugaa ggacaugcug     3240

aagcgcaaca cccccaucua cacccggcag gccgcgugca agaagggcgg ccucuucgac     3300

cagaccauca ugaagaaggg gcuggggcag cacccgcuga agaaggaggg ccccuucagc     3360

aacaucucca aguacggggg cuacaacaag gucagcgccg ccuacuacac gcucaucgag     3420

uacgaggaga aggggaacaa gauccgcucc cuggagacca ucccccugua ccucgugaag     3480

gacauccaga aggaccagga cgugcucaag agcuaccuga ccgaccugcu gggcaagaag     3540

gaguucaaga uccugguccc gaagaucaag aucaacuccc uccugaagau caacggguuc     3600

cccugccaca ucaccggcaa gacgaacgac agcuuccugc uccggcccgc cgugcaguuc     3660

ugcugcucca acgacgaggu gcuguacuuc aagaagauca uccgcuucag cgagauccgc     3720

ucccagcggg agaagaucgg caagaccauc agccccuacg aggaccugag cuuccgcucc     3780

uacaucaagg agaaccucug caagaagacc aagaacgacg agaucgggga gaaggaguuc     3840

uacgaccugc ugcagaagaa gaaccucgag aucuacgaca ugcugcugac caagcacaag     3900

gacacgaucu acaagaagcg gccgaacagc gccaccaucg acauccucgu caagggcaag     3960

gagaaguuca agucccugau caucgagaac caguucgagg ugauccugga gauccucaag     4020

cuguucagcg cgacccgcaa cguguccgac cugcagcaca ucgggggcag caaguacucc     4080

ggggucgcca agaucggcaa caagaucagc ucccucgaca acugcauccu gaucuaccag     4140

agcaucaccg gcaucuucga gaagcggauc gaccugcuca agguguga                  4188


<210>  2115
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2115
auggacagcu ccaagcaggg ccugcgccgg uaccaccucg ggcuggacau cggcaccaac       60

agcaucgggu ggaccgccau cgacgaccac uucucccugc uccgcgugaa gggcaagaac      120

gccaucgggg uccggacguu caaggagggc gagaccgcgg ccgaccgccg gggcuuccgc      180

accgcccggc gccggcugag ccgccggcgc uggcggcugc aguuccucga cgaguucuuc      240

gcccccuacc uggccgaggu ggacccgaac uuccuggcgc gccucaagca guccgacauc      300

agcgccaagg accccgccaa gaaccaggag uacaugggga agcugcuguu ccccgaccag      360

gaggccgcgu ccgacggcaa cggguacccc acccucaucc agaugcggaa ggccaugccg      420

gccgagaagg ccgccgacuu ccccguguuc aacaucuacc agcugcgcua cgcgcugaug      480

aacgagaagc ggaaguucga ccuccgcgag aucuaccugg ccguccacca caucgugaag      540

uaccggggca acuuccugaa caacacgagc guggacaagu ucaaggccga ccagaucgac      600

uucaacaagu ccuucgagac ccucaacgac cuguuccgcc agguccagcc caucgccccc      660

uuccagaucg accugaccaa gguggacgag cucgggaagc ugcugcucga caccaagcag      720

aagaagcugg accggcagaa ggccguggcg aagcugcucc cgcugaucga ggacggcacg      780

gacaaggaga ucgucaaggc ccaccagcag accgcccgcg agaucagcaa gcugauccuc      840

ggcuacaagg ccaagguggg gcugauccug cagaacaacc ucgaccagac ccuggacaug      900

uccaccgaga acagcgacga ccagcugaug cagaucgccg aggagcucga cgacuaccag      960

auggagcuga ucaaccagcu gucccuccug uacagccaga ucaugcugaa cgagaucgug     1020

cccaacggcg agacggucuc cgcgagcaug cucaaccgcu acuacaagca ccggugccag     1080

cugaaggagc ugaaggacua cggggccgcc caggacaaga agacccgcga gcagcucgac     1140

caccuguacg ccgaguacau cggccagauc cccaaggacu ccaaguucga cuucaccaag     1200

gaccugaaga agcucaugga caagagcgac cugggccaga agaucaagga cgagaucgag     1260

gccggggacu accugcccaa gcagcggacc uccgcgaacg gcgugauccc gcaccagaug     1320

caccagcagg agcuggacca gaucaucgag aaccagaagg aguacuaccc cuggcucgcc     1380

gagcccaacc ccguggagaa gaacaucaag aacgccaagu acaagcugga ccagcugguc     1440

agcuuccgcg ugccguacua cguggggccc augaucacgc ucgccgacca gcagaagacc     1500

agcaaggcgu ccuucgccug ggccguccgg aagaaggccg gcaagaucac ccccuggaac     1560

uucuacgaca ugaucgaccg cgagaagucc gccaacaacu ucaucaagcg gaugaccaac     1620

aaggacacgu accugcugag cgaggacgug cuccccgcga gcucccugcu guaccagaag     1680

uucaccgugc ucaacgagcu gaacaacauc cgcgucaacg ggcggaagcu gcagccggcc     1740

cucaagcagg ccaucuacac cgaccuguuc cagaagcgca agaccgugaa ccugaagacg     1800

cucaccaacu uccuggccau ccacgagccc acccugacca agcccaagau cgagggccuc     1860

agcaacggcc gguccuucaa cagcucccug gggacguaca ucgacaugag caagauccug     1920

ggcgacuccc ucgacgaccu gaacuaccgc gacgacaucg agaagaucau cgaguggagc     1980

accguguucg aggacuccga gaucuacggg gucaagcugc aggagaucca guggcucacc     2040

aacgagcagc gggagaagcu ggugcgcaag cgguaccagg gcugggggcg ccugagcaag     2100

aagcuccuga ccggcaucac ggacgagaac ggccagcgga ucaucgaccu gaugugggac     2160

accuccaaga acuucaugca gaucguccac cagcccgugu ucgaggagca gaucgccgag     2220

cucaaccagc agcugcugca ggacgcggac aaggccccgc ucgagguggu cgacgacauc     2280

cuggccgacg ccuacaccag cccccagaac aagaaggcca uccgccaggu ggugaagguc     2340

guggcggacg uggucaaggc cguggggaac gcccccgccu ccaucagcau cgaguucgcg     2400

cggaacgagg accgcaacaa ggagcugaag aacucccgcc ggcacagcau cgagaagcuc     2460

uaccacgaga ccgccgagaa gcuggugaag cagacgaacc ugcucgagga gcugggcaac     2520

gucaaggacc ugaacgaccg cuacuaccuc uacuucaccc aggccgggcg ggacauguac     2580

gacggcaccc ccaucaacau cgacgagauc agcacccacu acgagaucga ccacauccug     2640

ccgcaguccu uccugaagga caacagccuc gacaaccgcg ugcuggugaa gaaguccgag     2700

aacgccgcca agagcgaccg gguccccgcg aagcuguacg gggccaagau gcgcgccuuc     2760

uggaagcagc ucaaggaguc ccagcugauc agcaaccgga aguaccugaa ccucaccacg     2820

gaccccgagc aggucgacaa guaccagaug cagggcuucg ugcaccgcca gcugguggag     2880

acccggcaga ucaucaagcu ggccgccaac aucuucggcu cccucuaccc ggacagcgcg     2940

gugaucgaga cccgcgccga gcugaccaag cagcugcggg agaaguucuc ccucuacaag     3000

gugcgcgacg ucaacgacua ccaccacgcc guggacgccu accugacggc cuucgcgggg     3060

cacuaccugu accagcggua ccccaagcuc cgccccaugu ucguguacgg cgacuacgcc     3120

aagaucuacg ccgacgaccu gaagcggcug cgcagcguca accucuucca cgaccuggag     3180

aaggaccagu acaacuccgg gaccgccgug aaccccgaga ccaaggagaa gaucguggac     3240

gucgacuggc ugagcgacua cguguccaag auccagcacu ucaaguacau gcucaucagc     3300

aaggcggugu acacccagca cggccagcug uacggcgcca cgauccugcc ggucgcccag     3360

guggacaaga ucuccgcccc caucgggauc aaggacgaca agcccgugga caucuacggc     3420

gccuacacca acgaggucgc ggccuacaug gccaucgugc gggugaacgg gaagaagccc     3480

aaguacaagg ucgugagcgu gccgguccgc augcuccagc ggcugaagau ggccgagcag     3540

aagggccacg accaguacau gcagacccug cacgacgugc uccugcgcaa cuucaccucc     3600

cugaagaaga accggaagac ggggcucaag gaggccgugg ucaaggacuu cgacgugcug     3660

gugcccugcg uccgcuacaa ccagcuggug aucgacggcg acaccaaguu cacccucggc     3720

agcgcgaccu accagcacaa cgccaagcag cuggugcugu gcaaccacag ccucgagacg     3780

cuggccaaca acuucgagua cguccgcaag caccccgacg aggccgacga gcggaugauc     3840

augcuguacg acgacauccu cgaccaggug aaccacuacu ucacccugua cgacaagaac     3900

aaguuccgcg agaagcugaa cgccgggcgg gacaaguacc ucaagcugcc caccuucucc     3960

caguucgaga acaacaagaa gaccgcgauc ggcaagagcg aggccaucau caacauccug     4020

cgcgggcucc acgccaacgc cgccaucacg aaccugaagu gccugggcgu guccaccccg     4080

uucgggcagc uccagagccc cuccggcauc gagcugagcg agaacgcgaa gcuggucuac     4140

caguccccca ccggccuguu cagccgggag gugcuccuga aggaccugug a              4191


<210>  2116
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2116
auggccaacc ugcgcaacua caagggcgac uacaacaucg ggcucgacau gggcaccggg       60

agcgugggcu gggccgucac cgacuccgag gggaagcugc ugcacuucaa gaagcagccc      120

acguggggca gccgccucuu cgacuccgcg gagaccgccg ccagcgcccg gaucccgcgg      180

ggccagcgcc ggcgcuacgu gcggcgccgg uggcgccugg accugcucca gcagcuguuc      240

caggacgagg uguccaaggc cgaccccgag uucuucaucc ggcugcgcca gagccggcuc      300

cugaaggagg accgcgagga ggggcaccgg gacuacaacc acccccuguu caacgacucc      360

gacuucaccg agaccgacua cuacaaccgc uuccccacga ucuaccaccu ccggaagugg      420

cugauggaga ccgacgagaa ggcggacauc cgccuggucu accucgccgc ccacaacauc      480

gugaagcacc ggggcaacuu ccugcgccag gagaacacca gccuguccgc caagaacgcg      540

cggccgcucg acgcccugcg cgaguuccgc agcgcccugg uggacuggug cgccgagcuc      600

ggguacgagu gcaaccccga cgaguccggc gagaucagca acgagauccu guccguccug      660

gaggaggagc acguggccaa cagcgacaag aagaagcgga ucuccagccu caucggggug      720

gcgaugaacg agggcgccaa ggaguccaag gccugcaacg acgcccuggc cggcgcgaug      780

gucgggcuga aggccgaguu caagaacgug uucggcgaga ucagcgccga gcagaccucc      840

aucuaccuca gcaacgacga ggccguggac gcccugcgcg aggggauccc cgacgagggc      900

cuggagcugu ucggcaagcu cugcagcguc uacuccgcgu acgugcugca ggggcuccug      960

agcuacgccc ccggccagac gaucuccgug aacauggucg ccaaguacga gaaguacggg     1020

aacgaccuga agacccucaa ggcccuggug ugcgaguacg cccgggcgga guacgacagc     1080

uucuuccgcg gcgaguucua ccgggaccag gacgggcacg agcugaacau cuacaacgcc     1140

uccaaggccc agggcuacac ccgcuacaac cucggcacca gcaagauguc cuacgaggac     1200

uucgagaagg ccgugaagaa gcuguucgcg gggacggccg ccgaggagga cggccgguac     1260

cugcagauga ugaaggaguu cggggagcag cgcuuccucc ggcgccugaa gaccagcgac     1320

aacggcucca ucuacuacca gcugcaccuc gaggagcuga ccgccauccu gagcaaccag     1380

gggaagcacu acccguuccu cgucgaggac gccgagaaga ucaagucccu ggugagcuuc     1440

cggauccccu acuacguggg cccccugacc gaccgcaacg cgcggaagga caagcacggc     1500

aagaaccgcu ucgccugguc cgagcgcaag agcgggaugg aggacgccgu caucacgccc     1560

uggaacuggg acgaggugau cgaccggggc aaguccgccg aggccuucau cacccggaug     1620

accgggaugu gcaccuaccu gcagggcgag gacgugcucc cgaagugcag ccugcuguac     1680

gaggaguucu gcguccucaa cgagcugaac gggucccgcu ggggcaugga cggcgacaag     1740

gagcaccggu ucgacgcggc cgaccgcgag gccaucaucg aggagcuguu caagaagaag     1800

aagacgguga aguacaagga cgucgccaac uggcucgagc gcgaggccgg gcggaugaac     1860

gugcacguga ugggcgggca gggcgagacc ggcuacgaga gcaagcuguc cagcuacuac     1920

uucuuccgcc gggacaucuu cgacgucgag gagcuggacc gcgcggacua ccccaugauc     1980

gagcagauca uccucuggaa cacccuguuc gaggaccggc gcauccugaa ggagaagcuc     2040

gaggacgccu acgggcccaa gggcaacggg cggcuggacg acgaccagau caagaagauc     2100

ugccgcaagc gguucaccgg cugggggcgc cugagcaaga aguuccucac gggcaucaag     2160

guggacaccg acugcggccc ccgguccauc auggacgugc ugcgcgaggg ggacccgaac     2220

gccggcuucc gggggaagac cauggucaug auggaggugc ugcgcgacga gaaccucggc     2280

uuccagcaga agguggacga guucaacaag aacuacuucg ggcagaaggg caaggcccug     2340

gaggucaacg agcugcccgg cagccccgcg auccggcgcu cccucaacca ggccauccgg     2400

aucguggacg agaucgccaa gaucgccggg aaggaccccg agagcaucuu caucgaggug     2460

acccgcgacg acgagcugga caagcggggc aagcgcacga agcggcgcua cgacgcccug     2520

aaggaggcgc ucaccgucuu caagaaggag gacccggaga ucugggccga gcugcaggcc     2580

accuccccca gcgaccugga cgagcgccuc acccuguacu ucaugcagcg ggggaagugc     2640

cuguacuccg gcgagcccau cgacaucaac aagcucagcu ccggggagua cgagguggac     2700

cacaucaucc cccgcagcua caucaaggac gacucccugg agaacaaggc ccuggugugc     2760

cggaaguaca accagagcaa gacggacgcc augcuccugg acccguccau ccgccggaag     2820

augcgcagcu acugggacau gcugcucgac gcgaagcuga ucggccccaa gaagcaccgg     2880

aaccugcucc gcgaccacau cgacgagaag gccaugaagg gcuucgucgc ccggcagcug     2940

guggagaccu cccagagcgu gaagcuggcc gggucccucc ugagcguccg cuaccccaac     3000

gccaacaucg ugcccgugaa ggcgagcaug ucccacgacc ugcgggaggc cgccggcuuc     3060

gucaagugcc gcgaggccaa cgacuuccac cacgcgcacg acgccuaccu cgccugccgg     3120

auggggcugu ucauccagau gcgccacccg aagguguacg agaaccccau cgccaugacc     3180

cgggugaugc agaaguacgc ccagcagcag agcaaggagu ucaaccgcaa ccaccggguc     3240

cccggcaccu ccggguucau cgugaacagc uucaugucca gcggcuucga caaggagacg     3300

ggcgagaucu ucaaggacac cuggaacgcg gagaccgaga ucgaggggau ccgcaaggcc     3360

cugaacuacc ggcagugcua caucucccgc augccgaugg aggacaccgg cgccuucugg     3420

aacgccaccg uguacagccc ccgcgacccc aagauggggc ccaagcucgc ccugccgcug     3480

aagaagggcc ucgaccccaa gaaguacggc ggguucucca gccagcaguu cgcguacuuc     3540

uucgucuacg aggcccggga caagaaggac aagcaggucu uccgcuucgc cgaggugccc     3600

guguggcugg ccucccagau cgagagcgac aagaucgccc uggagcggua cgcgacgggc     3660

cucgccgagg ccgagaagcu gaaguucgug cgcaucgagc gggccaagau ccugaagaag     3720

cagcucaucg agaucgacgg ggaccgcuuc auccugaccg gcaagaagga gaugcggaac     3780

gggcgcgagc uggcccucuc ccagaaggag acccugaacc ugaucguggc gggcgacgag     3840

aacgccagcc ucgacgaccg gggcaacgcc uccgacgccg ucuucgacgc gaucgugcgc     3900

cagggggacg agaucggccu gcggcuguac aagcagcuca agaucgccga gaucgccgag     3960

cgcuaccgga gcgugggggc cgacgccaag gucggcgcgg ugaugucccu gcugagcucc     4020

aucaacgcca gcagcaacau ggugaaccuc uccauggucg ggggcagcaa guacgccggc     4080

aaccugcagc ccaacuacuc caaccugcuc agcgacccgg agaccaacuu cgugaucauc     4140

gaccaguccg ugacggggau guucgagcgc cggacccgca ucggccugug a              4191


<210>  2117
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2117
augaccaagc ccuacagcau cggccuggac aucgggacca acuccguggg cugggccguc       60

aucacggacg acuacaaggu gccggccaag aagaugaagg ugcucgggaa caccaaccgc      120

gacuacauca agaagaaccu gcugggcgcg cuccuguucg acgggggcga gaccgccgag      180

ggccggcgcc ugaagcggac cgcccgccgg cgcuacacgc ggcgcaagaa ccggcuccgc      240

uaccugcagg agaucuucgc cgaggagaug augcaggucg acgagagcuu cuuccagcgg      300

cuggacgacu ccuuccucgu ggaggaggac aagcagggga gcaaguaccc caucuucggc      360

acccugaagg aggagaagga guaccacaag aaguucaaga ccaucuacca ccuccgcgag      420

gagcucgcca acuccaagga gaaggcggac cugcggcugg uguaccucgc ccuggcccac      480

augaucaagu uccgcgggca cuuccuguac gagggcgacc ucaaggccga gaacaccgac      540

guccaggcgc uguucaagga cuucguggag gaguacgaca agacgaucga ggagagccac      600

cuguccgaga ucaccgugga cgcccucagc auccugaccg agaaggucuc caagagcucc      660

cggcuggaga accucaucgc ccacuacccc accgagaaga agaacacgcu guucgggaac      720

cugaucgccc ucagccuggg ccugcaggcc aacuucaaga ccaacuucca gcucuccgag      780

gacgcgaagc ugcaguucag caaggacacc uacgaggagg accuggaggg ccuccugggg      840

gaggugggcg acgaguacgc cgaccuguuc guguccgcca agaaccucua cgacgccauc      900

cugcugagcg ggauccucac cgucgacgac aacuccacga aggcgccccu gagcgccucc      960

auggugaagc gcuacaagga gcacaaggag gagcuggccg ccuucaagcg guucaucaag     1020

gagaagcucc cgaagaagua cgaggagauc uucaaggacg acaccaagaa cggcuacgcc     1080

ggcuacgugg gggccgacaa gaagcugcgc aagcggagcg gcaagcuggc gaccgaggag     1140

gaguucuaca aguacgucaa ggggauccuc aacaaggugg agggcgccga cuacuuccug     1200

gacaagaucg accgcgagga cuuccugcgg aagcagcgca ccuucgacaa cgggagcauc     1260

ccccaccaga uccaccucca ggagaugcac gccauccugc gccggcaggg cgagcacuac     1320

cccuuccuga aggagaacca ggacaagauc gagaagaucc ugacguuccg cauccccuac     1380

uacgugggcc cgcucgcccg gaaggggucc cgcuucgccu gggcggagua caaggccgac     1440

gagaagauca cccccuggaa cuucgacgac auccuggaca aggagaaguc cgccgagaag     1500

uucaucaccc ggaugacccu gaacgaccuc uaccugcccg aggagaaggu ccugcccaag     1560

cacagcccgc ucuacgagac guucaccgug uacaacgagc ugaccaaggu gaaguacguc     1620

aacgagcagg gcgaggccaa guucuucgac accaacauga agcaggagau cuucgaccac     1680

guguucaagg agaaccgcaa ggugacgaag gacaagcugc ucaacuaccu gaacaaggag     1740

uucgaggagu uccggaucgu caaccugacc gggcucgaca aggagaacaa ggcguucaac     1800

gccagccugg gcaccuacca cgaccugcgc aagauccucg acaaguccuu ccuggacgac     1860

aaggugaacg agaagaucau cgaggacauc auccagaccc ugacgcuguu cgaggaccgg     1920

gagaugaucc gccagcggcu gcagaaguac agcgacaucu ucaccaccca gcagcugaag     1980

gagcucgagc gccggcacua caccgggugg ggccgccugu ccuacaagcu gaucaacggc     2040

auccggaaca aggagaacaa gaagacgauc cucgacuacc ugaucgacga cggguacgcc     2100

aaccgcaacu ucaugcagcu gaucaacgac gacgcccuca gcuucaagga ggagaucgcc     2160

cgggcgcaga ucaucggcga cgucgacgac aucgccaacg uggugcacga ccugcccggg     2220

ucccccgcca ucaagaaggg cauccugcag agcgucaaga ucguggacga gcucgugaag     2280

gucauggggc acaaccccac caacaucauc aucgagaugg cccgcgagaa ccagaccacc     2340

gacaagggcc ggcgcaacuc ccagcagcgc cugaagcugc uccaggacag ccugaagaac     2400

cuggacaacc cggugaacau caagaacgug gagaaccagc agcuccagaa cgaccggcug     2460

uuccuguacu acauccagaa cggcaaggac auguacaccg gggagacgcu cgacaucaac     2520

aaccuguccc aguacgacau cgaccacauc aucccccagg ccuucaucaa ggacaacagc     2580

cuggacaacc gcguccucac ccgguccgac aagaaccgcg gcaagagcga cgacgugccc     2640

uccaucgagg ugguccacga gaugaagucc uucuggagca agcugcugag cgugaagcuc     2700

aucacccagc ggaaguucga caaccugacc aaggcggagc gcgggggccu gacggagaac     2760

gacaaggccg gcuucaucaa gcggcagcuc guggagaccc gccagaucac caagcacguc     2820

gcccaggugc uggacgcccg guucaacgcc aagcacgacg agaacaagaa ggugauccgc     2880

gacgucaaga ucaucacccu caagagcaac cucguguccc aguuccggaa ggacuucaag     2940

uucuacaagg ugcgcgagau caacgacuac caccacgcgc acgacgccua ccugaacgcc     3000

gucaucggga cggcccugcu caagaaguac cccaagcugg cgagcgaguu cguguacggc     3060

gaguucaaga aguacgacgu gcggaaguuc aucgccaagu ccgacaagga gaucgggaag     3120

gccaccgcca aguacuucuu cuacagcaac cugaugaacu ucuucaagaa ggaggucaag     3180

uucgccgacg gcaccguggu ggagcgcccg gacaucgaga ccuccgagga cggggagauc     3240

gcguggaaca agcagacgga cuucaagauc guccggaagg ugcucagcua cccccaggug     3300

aacaucguca agaagaccga ggugcagacc cacggccugg accgcggcaa gccccggggg     3360

cuguucaacg ccaaccccuc cccgaagccc aagcccgaca gcuccgagaa ccucgugggc     3420

aucaagcgca accuggaccc caagaaguac gggggcuacg ccgggaucag caacuccuac     3480

gccguccugg ugaaggccau caucgagaag ggcgugaaga agaaggagac caugguccuc     3540

gaguuccagg gcaucagcau ccuggaccgg aucacguucg agaaggacaa gcgcgcguuc     3600

cugcucggga agggcuacaa ggacaucaag aagaucaucg agcugccgaa guacucccug     3660

uucgagcuca aggacgggag ccggcgcaug cuggccucca uccugagcac caacaacaag     3720

cgcggcgaga uccacaaggg gaacgagcug uucgugcccc agaaguucac cacccugcug     3780

uaccacgcca agcggaucaa caaccccauc aacaaggacc acaucgagua cgugaagaag     3840

caccgcgacg acuucaagga gcuccugaac uacguccugg aguucaacga gaaguacgug     3900

ggcgccacga agaacggcga gcggcucaag gaggccgugg cggacuucga cuccaagagc     3960

aacgaggaga ucugcaccag cuuccugggg gccgucaacu ccaagaacgc cggccuguuc     4020

gagcucacca gccugggguc cgccagcgac uucgaguucc ugggcgugaa gaucccgcgc     4080

uaccgggacu acacccccuc cagccuccug aaggacucca uccugaucca ccagagcauc     4140

acggggcucu acgagacccg caucgaccug uccaagcugg gcgaggacug a              4191


<210>  2118
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2118
augaagcgcg ugaacgagga cuacauccug ggccucgaca ucgggaccaa cagcugcggc       60

ugggccguca ccgacaagaa gaacaaccug cugaagcucc gggggaagac ggccaucggc      120

ucccaccugu ucgaggaggg gcacaccgcg gccgaccgcc ggggcuuccg caccacccgg      180

cgccggcuga agcgccggaa guggcgccuc cggcugcugg aggagaucuu cgccgagccc      240

auggccaagg uggacccggg cuucuucgug cgccuccacc agagcugggu cagcccccug      300

gacaaggacc ggaagaagua caacgccauc guguucccca cggcgaagga ggaccaggcc      360

uucuacaagc acuacgccac caucuaccac cuccgcgacg agcucaugac ccaggaccgg      420

caguucgacc ugcgcgagau cuuccuggcc auccaccaca ucgugaagua ccgggggaac      480

uuccuccagg acacccccgu caaggacuuc gaggcgagca agaucgaggu gggcccgauc      540

cugucccaca ucaacaacgc cuucgccgag aagaucgugg aggaccagga ccccaucgag      600

cugaacgucg ccaacgccgc ggacaucgag gacgugaucc gcgggaagga cgccgagaag      660

acgguguaca agcucgacaa ggucaagaag aucgccaagc ugcugaccga cagcaccgcc      720

aaggaggaga agaacguggc caagcagauc gcgaacgcca ucaugggcua caagacccag      780

uucgagacga uccucgacaa ggagaucgac aaguccgaca aggcccagug ggaguucaag      840

cugagcgacg ccgacgccga cgacaagcug gacgcgcucc ugcccgaccu ggacgagacc      900

gaccagaccg uggucgccga gaucgagaag cuguucuccg ccaucacccu gagcacgauc      960

guggacgaga acaagucccu gagccagucc augguggaga aguacaagaa gcacaagaag     1020

gacuacaaga agcucaagaa guacaucaac acccugcagg accagaccaa ggccaagaag     1080

cugcuccugg ccuacgaccu guacgucaac aaccggcacg ggcgccuccu ggaggcgaag     1140

aagaccuucg aggacaagaa gaagaagaag gcccugacga aggacgaguu cuacaagauc     1200

gugaaggaca accucgacga cagcgaccug gcccacgaga uccagcagga gaucgccgcg     1260

gacaacuuca ugcccaagca gcggaccaac uccaacggcg ugaucccguu ccagcugcac     1320

cagaucgagc ucgacaagau caucgccaac cagggcaagu acuaccccuu ccuggccgcc     1380

gagaaccccg ucgaggacca ccgcaagcag gcccccuaca agcuggacga gcucgugcgc     1440

uuccgggugc cguacuacgu cgggcccaug aucaccgcgg acgagcagga gaagaccagc     1500

ggcaaguccu ucgccuggau ggugcgcaag gaggacgggc agaucacgcc cuggaacuuc     1560

gagcagaagg ucgaccggca ggagagcgcc aacaaguuca ucaagcgcau gaccaucaag     1620

gacaccuacc ugcuguccga ggacgugcuc cccgccaaca gccugcugua ccagcgguuc     1680

gaggugcuga acgagcucaa caacauccgc aucaacggca gccggaucuc cgucgaccug     1740

aagcagcaga ucuucaacga ccuguucgag gagaagaaga ccgugacgga gaagucccuc     1800

accuccuacc ugaagcagaa ccugcaccuc ccgaccgugg agaucaaggg ccuggccgac     1860

cccaccaagu ucaacagcuc ccuggcgagc uacuaccacc ucaagucccu gcacgucuuc     1920

gacaaggagc uggccgaccc ccaguaccag aaggacuucg agaagaucau cgaguacagc     1980

uccaucuucg aggacaagaa gaucuuccag gacaagcucc acgccgaguu caaguggcug     2040

acgcccgagc aguucaaggc caucagcacc uggcgccugc aggggugggg ccggcucucc     2100

cgcaagcugc ugguggagcu ccacgacacc aacgggcaga acaucaugga gcagcugugg     2160

gacagccaga agaacuucau gcagaucgug accgagccgg acuucaagga cgccaucgcg     2220

aaggagaacc agaacgucac gcgggccaac ggcguggagg agauccuggc cgacgccuac     2280

accucccccg cgaacaagaa ggccauccgc caggugguca aggugguggc cgacgucgug     2340

aaggccgccg ggggcaagaa gcccgcgcag uucgccaucg aguucacccg ggaccccgac     2400

aagaacccgc agcucagcca cauccgcggc accaagcugc ugaaggccua ccaggagacc     2460

gccggggagc ucguggacca gaagcugacg gacucccuga aggaggccau gaccagccgg     2520

aagcuccuga aggacaagua cuuccuguac uucaugcagg cgggccgcga cgccuacacc     2580

gggcagaaga ucaacaucga cgaggucucc accaacuacc agaucgacca cauccucccc     2640

cagagcuuca ucaaggacga cuccuucgac aaccgggugc ugacggccac gccccugaac     2700

gccgagaagu ccgacgacgu gcccuacaag cgcuucgcca acaacuacgu cagcgacaug     2760

aagaugaccg ugggcgagau guggaagcac uggcagaagg cggggaucau caacaagcac     2820

aagcucggca accugcugcu cgacccggac cggcugaaca aguuccagaa guccggcuuc     2880

aucaaccgcc agcuggugga gaccagccag aucaucaagc ucgucagcgu gauccugcag     2940

aacaaguacc ccgacgccga gaucaucacc gugaaggccg gggacaacag cgcccugcgc     3000

cagcggcuca accuguacaa gucccgcgac gucaacgacu accaccacgc gaucgacgcc     3060

uaccugagca ucaucugcgg caacuuccuc uaccaggugu accccaagua ccggccguac     3120

uucguguacg ggaaguacaa gaaguucucc caggaccccg accugcagaa ggaggucauc     3180

aagcacuuca agggcuucac cuucaugugg ccccugcucc agaaggacaa cagcgagcgc     3240

aaggcccccg agaagaucaa ggagaacaac uccgaccgga ucguguucua caagcacccg     3300

gacaucuucg acaagcugcg caaggccuac aacuacaagu acaugcuggu gagccgggag     3360

accaccacgg agaacuccgg ccucuucgac gucaccaucu acccccgcgg ggagcgggac     3420

cuggccaaga cccgcaagcu gauccccaag agcaacggcc ucgaccccaa gaucuacggg     3480

ggcuacuccg ggaacaccga cgcguacaug gugaucguga agaucgacaa gggcaaggag     3540

agcaucuaca aggucaucgg cgugccgaug cgggcccugg ccucccugaa ccgcgccaag     3600

aagcagggga acuacaagga ggagcuccac caggugcugg agccccagau cauguucgac     3660

aagaacggca agcccaagcg gagcgucaag ggguuccgca ucaucaagga ccacgugccc     3720

uucaagcagg ucgugcugga cggcgacaag aaguucaugc ucaacuccag cacguacgag     3780

aucaacgcca agcagcugac ccugaccccg gagaccaugc ggaucgugac ggacaaccuc     3840

aagaaggggg aggaccagga ccagcugcug gugaaggcgu acgacgagau ccuccagaag     3900

gucgaccagu accugccccu guucgacgug aacaaguucc gcaacagccu ccaccugggc     3960

cgggccaagu uccuggaccu cgccgugaac gacaagaaga ucacccugac caacauccug     4020

aacggccucc acgacaaccu ggucaccccc gaccugaaga acaucgggau caagacgccc     4080

cucggcaagc uccaggugcc guccgggauc gugcugagcu ccgaggccau ccugaucuuc     4140

cagagcccca ccggccuguu cgagaagcgc guccggaucg ccgaccugug a              4191


<210>  2119
<211>  4194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2119
augaaggacu acaacaucgg ccuggacauc gggaccagcu ccaucggcuu cgccgccauc       60

gacgacgaca acaaccucau ccgcgugaag gggaagaacg ucaucggcag ccggcuguuc      120

gcggagggga agaccgccgc cgagcgccgg ggcuuccgca cggcccggcg ccgguacaac      180

cgccggaagu ggcgccugaa gcuccuggac gagaucuucu acgaggagau cauccacauc      240

gaccccgagu ucuucaaccg gcugaagcag uccagcaucu ccccgaagga cgagagcaag      300

aaguacuucg gcucccuccu guuccccgac aagaccgaca gcgccuacua ccacgacggg      360

aaccagacca ucuaccaccu ccgcaaccgg cucgugcacc agaaggagaa ggcggacauc      420

cgcgagaucu accuggcccu gcggcacauc gugaaguacc gcggcaacuu ccucgacucc      480

acccccguca gcuccuucga ggccgacaac cugaagcugg acgagcucgu gcccgugauc      540

aacgacaugu acgagaacau cgacaucgac uucagcauca acgucgacaa caucuccgag      600

aucgagaagg ugcugcugga caaccaccuc aagagcgccg acaagcagga ccggcugaag      660

gagcugcucu acgacaaccc ggaguacccc gacgacaagg ugaagcagaa ggagaucucc      720

aaguuccgca aggaccugga caaggagauc aucaagguca ucguggggua caaguucaac      780

gcguacaaga ucgugaacua cauccccgac gacggcagca aguucuccuu cagcuucucc      840

gacgagagca gcgacgacaa gcuggacgac cucuccgagg uccuggacga cagccagaug      900

gagauccuga acacgcucaa ggugaucuac ucccgggugc gccugaacca gaucaucccc      960

aacgggaugg gccugagcga guccaugguc gccaaguaca accggcacca cgagcagcuc     1020

gaggaccuga aggagcugcu cgacgugcug ccgaacgacg agaagcgcag caucgaguac     1080

gccuacaccg ccuacaucgg caacguggac aagcagaagu acaacaacaa ccuggucgcc     1140

ucccucaagg accugcccau caagggggac aucaacaaga agauccaggc gguguucgag     1200

uacgccaagg gccacaucag cucccaggac cuguacgacg ugaucaagac caucaucauc     1260

gacaaguacc ucgaggacga gagcuucgag uaccacgacc uggucgagcg caucuccaac     1320

gacaucgaca acaaggacua ccugcucaag cagcggagcu cccagaacgg gaacaucccc     1380

caccaggugc accagaugga gauggacaag aucaucgcca accagagcca guacuacccc     1440

uuccugggcg ugcugaaccc gaacgagucc cgccggcaca ucgccaagua caagaucagc     1500

gagcucgucu ccuuccgcgu gcccuacuac gucggccccc ugaucacccc cgaggagcag     1560

gagaagacga gcgggaagca cuucgccugg augaagcgga aggaggcggg caagaucacc     1620

ccguggaacu ucgaggagaa gguggacaag gaggccaccg ccgccaaguu caucaagcgc     1680

cugaccguga aggacacgua ccuccugaac gaggacgucc ugcccgacaa cucccugcuc     1740

uaccagcagu ucaaggugcu gaacgagcug aacaucguga aggucaacaa caagcggcuc     1800

acccugagcg agaagcaggg gguguacaac gaccuguuca agaacaccaa gagcaucacc     1860

gugaagcgcc ucaagaacua ccugaucucc aacuacaagc accuggagaa caucgagauc     1920

acgggccuca gcgacgggga caaguucaac aacucccugg gcaccuacaa cgacuucaag     1980

aagaucuucg gcgcccggau cgacgacguc aacuacagcg cggacuucga gaacaucauc     2040

gaguggucca ccguguucga ggaccgcagc auccugaagg agaagcucga gaaggacauc     2100

gacuggauca cccccgagga gaucaaggcc guggucgcca agcgguacuc cggguggggc     2160

cgccugagcg ccaagcugcu cacggagcug cuggacgacg acgggcagcg gaucaucgac     2220

auccucugga acgacuccau caccuucaug gaggccguga gcaagcccga gaucaaggcg     2280

cagauccaga acaucaaccg cgacuucauc aaggccaccg acaucgagac cgugcuggac     2340

gaggccuaca cguccccgca gaacaagaag gccauccggg aggucuacaa gcuggugaag     2400

gacauccaga aggcgauggg cgggaaggcc cccagcucca ucagcaucga guucacccgc     2460

ucccccgagg acaacggcag cgugaccaag acccgguaca acaacaugca gaacaaguac     2520

aaggagcucg gcaaggagcu guccgacgac cugagcgccc agcucaagga guacaagggg     2580

cgcauggacc aggacaaguu cuaccuguac uucaugcaga ugggcaagga ccuguacgac     2640

gggagcacga ucaaccucga cgaccugaac uccuaccaga ucgaccacau cgucccccag     2700

agcuucuaca aggacgacuc caucgacaac cgggugcuga cccacuacac caacaaccag     2760

aagaagggca accacacccc gcucaacggg cugaacaucg acgcccagac gcagaaccug     2820

uggaagcacc ucaacaagcu gggccugauc agcaagaaga agcuccagaa ccugugcuuc     2880

aaggacauca accagcuguc caaguacacc aagcacggcu ucgugcgccg ccagcucguc     2940

gagaccagcc aggugaucaa gcugguggcc aacauccugu ccgacgaguu cgcgaacgac     3000

gggaccaaga ucaucgaggu caaggccaag augaacagcc ucaugcggga cacguucaac     3060

cuguacaagg ugcgccagcu gaacgacuac caccacgccg uggacgccua ccucaccacc     3120

uucgucggca aguaccugua caagcgguac cccaagcugc gcuccauguu cguguacggg     3180

gacuucaaga aguucagcgc cgaggacgac cucaaggaca ucaagaccuu caacuuccug     3240

cacgacauca ccaaccccaa ggaguccaac aaggagaaga ucuacgacga gaaguccggc     3300

gagcugaucc ucgaccggaa gcacgcgauc gagacgauca agaagaucua ccaguacaag     3360

uucaugcuga ucgugcgcga ggucagcacc aagagcggcc agcuguucaa gcagaccauc     3420

uacuccgcca agaagaccaa cgggcucaag agcgccaucc ccgugaaguc caaccuggag     3480

ccgagcaucu acggcuaccg guccgggaac guggaccacc acauggucau cgugaagauc     3540

aagaagggca cgaaguacag cuucaaggug aucgggauca gcgcccgcgc cauccaggag     3600

cuggagaucg cgaagaagau cggcaacuac cgggacaagc ucuacgaggu cguguccgac     3660

cgccugagcc aguccaugaa guccguggag agcuuccgga ucauccugga caacaucaug     3720

uacggccagc ucaucgucga cgggaacgac aaguucaccc ugggcagcgc cgacuaccgc     3780

aagaacuccc ggcagcuggu gcucugcacc aacagccuga agauccugaa ggacgaggac     3840

uacaugaacc gccucuccga cauccagcgg agcaagcuga uggugcaggu cuacgacgag     3900

aucaucaaga ucaugaagaa caaguacucc cuguucauca gcaacaccga gaagcuccug     3960

gagaagcgcg acaucuucug cgggcugucc uucgacgagc agaagaucgu gaucuggaag     4020

aucaucaucg accuccacaa cggccccuuc acgaagaucc aggagcuggg gaucaacaac     4080

aagaugggca ccuuccagac caagcacggc aucauccuga ccgagaacgc caucgugauc     4140

uaccagagcc ccuccgggcu guucgagcgg aagguccgcc ugaaggaccu guga           4194


<210>  2120
<211>  4194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2120
augaagaagg ugcugggccu cgaccugggg accaacagca ucggcugggc ccuggucaac       60

gaggccacca aggaggagaa gaaggagggg aaguccagcg agaucaucaa gcucggcgug      120

cgcgugaacc cccugacggu cgacgagaag accaacuucg agaaggggcg gccgcugucc      180

accaacgcgg accgcacccu caagcggagc gcccgccgga accugcagcg cuacaagcug      240

cggcgccgga accucaucga gauccugauc aagaacggcu ucaucacgaa ggacacgccc      300

cugaccgaga ccggcaagga cacgacccac cagacccuca agcugcgcgc caaguccgcc      360

aaggagaagg uggaccugaa ggacuucgcc aagauccucc ugaccaucaa caagaagcgg      420

ggguacaaga gcucccgcaa ggugaacagc gacgaggacg gccuggcgau cgacgggaug      480

gccgucgcca agcaccucua cgagcagaac cugacgcccg gccaguacgu gcugaaccuc      540

cugcagaaga acaagaagua cgugcccgac uucuaccggu ccgaccugca ggaggaguuc      600

aagaacaucu ggaacaacca gaagaccuac uacuucgaca uccucaccga cacccuguuc      660

gaggagcuga aggacaagaa caagagccag acgugggcca ucugcaagaa gccguucgac      720

aucgcgggga ucaagcagau cggcaaggcc uccgacaagc gccucgagcg guacaagugg      780

cgcaccgagg gccugaccca gaagcugaac cucgaguacc uggccaucgc ccugcaggag      840

aucaacaacg acaucaacaa gagcuccggg uaccucggcg ccaucagcga ccgguccaag      900

gagcuguucu ucaacaagga gaccgucggg gagaaccugu accgccagcu cgugaagacg      960

ccccacacca gccugcggaa ccagguguuc uaccgccagg acuaccugga cgaguucgag     1020

accaucuggg agacccaggc gaaguucuac ccccagcuca cgcacacccu gaaggaggag     1080

auccgggacg ucaucaucuu cuaccagcgc aagcugaagu cccagaaguc ccucaucagc     1140

uucugccagu ucgagagcug ggagcagacc uacaucgaga aggagaccgg caagagcaag     1200

acgcgcaccg ugggccggaa ggugaucccc aaguccagcc cgcuguucca ggaguucaag     1260

aucuggcaga accugaacuc ccucgaguuc cgcaacagcg acgaggggga gaucaucgag     1320

uucaucaagc uggacgagga cauccggcag accgucuucg aggagcugaa ccuccgcggc     1380

aaccugaagc ccaacgacgu gcugaagauc cuggggaacu acaucucccu cggcaaggug     1440

agccagugga agugcaacuu cgaggagauc gaggggaacc ggaccaacgc cgcccuguac     1500

aacgucuacc agaucaucgc cgagaacgag ggcuacggcu ucgacugggc caagaagacg     1560

gcguccgaga ucaacaagga gcugaagaac aucuuccccc agcucgggau cgacgccagc     1620

auccuggacu ucgacuucac ccaggccaac uucgacaagc agaccuccua ccagcugugg     1680

caccuccugu acagcgccga ggaggacuuc aagaucuccg aggaggacaa ggugcuguac     1740

ggcaacagcu ccgugagccu caagaagaag cugcaccaga aguacggguu caagcccgag     1800

uacaccaagc ugcucacgaa caucucccug cagccggacu acggcaaccu gagcgccaag     1860

gcgaucugca acauccuccc cuaccugaag gagaacaacg ucuacuccga ggccugcgac     1920

cugaccgggu acaaccacag caacuuccuc accgccgagg agcaggccaa gcgcccccug     1980

aagcccaucc uggagcuccu gaagaagaac ucccugcgga acccgguggu ggagaagauc     2040

cucaaccaga uggucaacgu ggucaaccag gugaucgaga ccuacggcaa gcccgacgag     2100

auccgcaucg agcugacccg ggagcugaag aaguccgcca aggagcgcgc gaacaugacc     2160

aagagcaucg ccgacgccac ccggcgcaac gacgacuacc ggaagaccau caucaaggag     2220

uucggcaucc ccaaccccac caagagcgac gugguccgcu accggcucug gcaggagcug     2280

gccagccgcg gguacaagga caucuucacg gacaccgaga ucaagccgca ggagcuguuc     2340

uccaacaaga ucgacaucga gcacaucauc cccaaggcgc uccuguucga cgacagcuuc     2400

uccaacaaga cccuggccuu caagaaggac aaccucaaga aggccaaccg gaccgccuac     2460

gacuucaucg ccaacgacua cgcgcccaac cuggagaacu acaucgagcg cguggagagc     2520

cugcacaagg accggaagau cacgcgcgcc aagcgcaaca agcuccugau gcugcagucc     2580

aagcuccccg acggcuucau cgagcgggac cugcgcaaca gccaguacau caccaaggag     2640

gccaagaaga ugcuguacga ggugugccgg accgucgugu ccaccagcgg guccaucacg     2700

gacaagcucc gcgaggacug ggaccugauc aacgugauga aggagcugaa cuucccgaag     2760

uacaaggccc ucggccugac cgagaagauc gagcgguacg acaucggggc cgagaagaug     2820

aaggaggucg agaucaucga ggacuggaac aagcgcaacg accaccggca ccacgcgauc     2880

gacgcccuga ccguggccuu cagcacccac ucccacaucc aguaccucaa ccaccugaac     2940

gcccgccggg acgagggcca caagaagcac gccgcgauca ucgccaucga gagcacgauc     3000

accgagaagg agggcaacaa gaagcgccgg uucaucgcgc cccugcccaa cuuccgccuc     3060

caggccaagg agcacaucga guccauccug gugagcuuca agaacaagaa caaggucgug     3120

accaacaaca ucaacaagac caagcggauc aagaaggaca aguucaacac gaaggugcag     3180

cugacgcccc gcgggcagcu ccacaaggag accaucuacg gcaagaucaa gcagccgcug     3240

gagaagcaca ucaagcugaa caaguccuuc acccucgagc aggccaagcu gaucaucgac     3300

aagcggcagc gcgagaaggu ccugaaccac cucgagaagu acgcccacaa cagcgacauc     3360

gccuucaaca gcaagacccu gaagaagaac ccccugcuca ucuccgacga gccccugaag     3420

gaggugcugu gcuucgagga gguguucacc auccggaagg acaucagcua cgacaacuuc     3480

aagaacgaga agcagcucga caaggucauc gacgagaagg ugaagcaggc ccugaaggag     3540

cgcaucaacc aguacaacgg ggacuuccgg gaggccuucu ccgacaucga gcgcaacccg     3600

aucuggcuga acaaggcgaa gggcaucugc gugaagcggg ccaccaucaa gggcgucagc     3660

aacgccgagc cccuccacac cgccaagaac caccuggggg aggaggugcu gacggagaac     3720

aaggagcaga uccccgugga cuacgucucc accggcaaca accaccacgu ggccaucuac     3780

gaggacgagg acgggaaccu ccaggagaag guggucagcu ucuacgaggc ggugauccgc     3840

gugaacgagg gccugcccau caucgacaag gaguacaaca agucccuggg guggaccuuc     3900

aaguucacca ugaagcagaa cgagauguuc gucuucccga gcgaggacuu caaccccacg     3960

gagaucgacc uccuggacca gaagaacgcc ucccugauca gccccaaccu cuuccgcgug     4020

cagaaguuca ccaucaagga cuacuucuuc cggcaccacc uggagaccac cguggagaac     4080

aacaacacgc ugaagaacac caccuggcgc cgggagggcc ucuccggcau cuacgggauc     4140

aucaagaucc gccugaacca ccugggcgag aucguccagg ugggggagua cuga           4194


<210>  2121
<211>  4194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2121
augaacgaga acaaggagug guuccugggc cucgacaucg ggaccgccag cgugggcugg       60

gccgcgaccg acacgaacua caaccugcug aagcucaagg ggaagaaguc cuggggcguc      120

cgccuguucg acgaggccca gaccgccaag gagcggcgcg acugccggag caagcgccgg      180

cgccuggccc ggcgcaagug gcggcucggg cugcuggagg agcuguucgc cgaggagauc      240

aacaagaucg accccaacuu cuucaugcgc cuccgggagu ccaaguacaa ggugagcgac      300

aagaccggca acgugccguc cauccuguuc aacgagaaga acuacaccga caaggaguac      360

uaccgcaacu acaagacgau cuaccaccuc cgggcggacc ugauccacaa gaacaacccc      420

gacauccgcg agcuguuccu cgccauccac cacauccuga aguaccgggg ccacuuccug      480

uucgccgggc agcaguucaa gggcagcgag aucaaggaca acauccgcga gcuccugcag      540

uacaacuccc ugaacaucaa gaucgacauc accgacgagc ucaucaacaa caucgccgag      600

auccugcuga agaagaagaa cagcaccgac aagaucaucg aguucaagaa gcuguucgcg      660

gacgagaagc agcucaucga ggcccugcgg cucgcccugg ggaacaagac ccagcuggcc      720

aagcuguucg acaucgaggc cuacaaggag cucgacaucu ccaucaagac gaucagcuuc      780

aaggagaaga ucuacgagga cguccgcucc aacuacgagg agauccuggg cgaguacauc      840

aucgugcucg acaccgcgaa gcugaucuac gacgccauca uccugagcaa gaucaagauc      900

aagaacauga ccaucuccga gagcaaggug gucuccuacg agaagcacgg gaaggaccuc      960

aagcagcuga agcaccugau caaggaggac ugcaagcuca gcaagaagga gaagcggcag     1020

cuguucgaga acaucuucgu gaaggaggac gagaagggcg ccaacuacgu gaacuacauc     1080

ggcaagaaca acgccggguc cagcaagucc agcugcagcu acgaggacuu caagaaguuc     1140

cugaccaagg agcucaagug cugcgaggag uccaagcuga agcaggacau ccuggccgag     1200

cucgagcugg agacguuccu gccccgccag cggaccaagg acaacagcgu cauccucuac     1260

cagauccaca aggaggagcu ggagaagauc cugaacaacg cggugcagua ccaccccuuc     1320

cugaacaaca ccgacgagua cggcaucucc aucaaggaga agaucaucca gcuccuggag     1380

uuccgcaucc cguacuacgu ggggccccug aacaccagcc acaaggucgg cgacggcggg     1440

uucuccugga gcgugcgcaa caagggcuac gagcacaucg ccgugacgcc cuggaacuuc     1500

aacaagguca ucaacaagga ggaguccgcc aagaacuuca ucgagaaccu caccaaccag     1560

ugcaccuacc ugcuguacga ggacgugcuc cccaagcaca gccugcugua cuccgaguuc     1620

gcccuccuga acgagcugaa cagcaucaac uacgggggca agcggcucac cguggagcac     1680

cgcaaccuga ucaucgagcg guucuucaag caguccgccc cgaaggcgac ggggaaggag     1740

cugaagaagu uccucaugag cgagggcguc aucgacggca aggacgagcu guccggguuc     1800

gacgugagca ucaagaacga ccugaagucc uaccgcgacu ucaagaagau ccucggcgac     1860

aaguucgaca gcgagaaggu ggagaagaug auccggugga ucacccuguu cggggagucc     1920

aagcagcugc ucgucgacaa gaucaccaag gaguacggcg acaucuacag ccuggaggag     1980

aucaagaaga ucuccgggcu gaccuacaag gacuggggcc gccucagcga gaagcugcug     2040

acggagaucg ugagcuccca gcucuacgac gagagcaccg gcgagugcuu caacaucauc     2100

aacgccaugc ggaacaacac ccugcuguuc auggagcucc uggggaccaa cuacgacuac     2160

uucgagaaga ucaacaucua caaccgccgg cugcgcaagg agaucacgga gaucacgccc     2220

cacauccucg acgaccugua cguguccccc gccgucaagc ggagcaucug gcaggucgug     2280

cgcaucgugg aggagcugaa gaagaucauc ggcuccgagc ccaagcggau cuucguggag     2340

accacccgca gcaacaagga gaagaagggg gacgacggcc ggaaggacuc ccgcaagaag     2400

gcccuccugg agcuguacaa gaagaucaag accagcgaca ucgucgagcu cgagaaggcc     2460

cuggggcaca ccguggacuu cgacaagaag aagcaggacc ugaccgacau cgacaacaac     2520

aagcucaagg cgcggaagcu guaccuguac uacacccagc ucggccgcug ccuguacacg     2580

ggcaaggcca ucgaccugaa caaccuccug uccagcaacu ccgaguacga cguggaccac     2640

aucuacccgc agagcaagac caaggacggg uccuggaaca accugguccu cgugaacaag     2700

accgagaaca gcaagaaguc caacggccug aucagcgagu ccauccagcg gaagaacaag     2760

cacuucuggc gcuuccugaa ggccaagaac cucaucagcg aggagaagua caagcgccug     2820

acccggaccg aggacuucac ggacgacgag cugagcgggu ucaucgcccg ccagcucauc     2880

gagaccuccc agagcgugaa ggcggucucc cagauccugc agaaccugaa ccccaccacc     2940

gagaucugcu ucagcaaggc cgagaacgug cacgacuucc ggcaggaguu cggcaagauc     3000

aaggugggca agcgccggaa ccucacggag gagcagcaga aggaggccaa ggaggggcag     3060

cugaucaagg uccgcgaccu gaacgaccuc caccacgcca aggacgccua ccugaacauc     3120

guggugggca acgucuacca caucaaguuc acccggaacc ccaagaacuu cauccgcaag     3180

aacuccaaga accgggagua cacccugaac acccucuucu acagcgacaa gaucgagcgc     3240

aacggggagg uggcguggga gaagggcaag acggagaaga ucguguccag cgucaugugc     3300

aacaaccuga ucaacgugac ccggcgcacc uccgagcaga agggggcccu guacgacgcc     3360

accaucuaca aggccaacgc cgcgaaggag ggcagcuacu accccgugaa gacgaagaac     3420

aagaaccuca aggacgucac ccgguacggc ggguucaccu ccaucaagau cgccuacuac     3480

agcaucuuca aguacgacau ccgcaacaag gacaagguga aggagaacau caagaucauc     3540

ccgaucccca ucuacaucuc ccagaacauc aagaccgacc uggaccugau caacuucgcc     3600

gagaagcaga ucaagcucaa gaagggcgag aacaucgaga accugcagcu gaucuacaag     3660

aagcucaaca ucggggcccu ggugaagauc gacuacuacc ccuacuacgu cggcgggaag     3720

acgaacgaca acuucuacau cgacggcgcc guccaggugc ugcucagcuc cgacaucgag     3780

cgguaccuga aggagguggu gaaguucgug aacuuccagc gcgagaacaa ggaggcgaag     3840

gucagcaagg agcacaucuc cagcgaggag aacaucgagc uguacaacac ccuccugugc     3900

aagaugaccg ccaagaucuu cacccacacg aagaacaaca aguacaacga ccuggcccag     3960

gacaaggcca agaacaccuu caagaaccuc ucccuggagg agcagugcaa ggugcugcuc     4020

gagauccuga accugcucac caacaagaag accacguucg accugagccu gaucaacgag     4080

aaggcgagcc gggugaagug cggcuucaac cuccugaacc agaagcaguu cuccaucauc     4140

gagcagagcg ucaccgggcu guacgagaag gagaucaaga ucaucgagga cuga           4194


<210>  2122
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2122
auggagaccc aggccagcaa ccagcugauc uccagccacc ucaagggcua ccccaucaag       60

gacuacuucg uggggcugga caucggcacc uccagcgucg ggugggccgu gacgaacaag      120

gcguacgagc ugcucaaguu ccgcucccac aagauguggg gcagccggcu guucgacgag      180

ggggaguccg ccguggcccg ccggggcuuc cgcagcaugc ggcgccggcu ggagcgccgg      240

aagcuccgcc ugaagcugcu cgaggagcug uucgccgacg ccauggcgca ggucgacccg      300

accuucuuca ugcggcugcg cgaguccaag uaccacuacg aggacaagac caccggccac      360

agcuccaagc acauccucuu caucgacaag aacuacaacg accaggacua cuucaaggag      420

uaccccacgg uguaccaccu ccggagcgag cugaugaagu ccgggaccga cgacauccgc      480

aagcuguucc uggccgugca ccacauccug aaguaccggg gcaacuuccu cuacgagggg      540

gccaccuucg acagcaacgc cuccacccug gacgacguca ucaagcaggc gcuggagaac      600

aucacguuca acugcuucga cugcaacagc gccaucucca gcaucggcca gauccucaug      660

gaggccggga agaccaaguc cgacaaggcc aaggccaucg agcaccuggu ggacaccuac      720

aucgcgaccg acacggugga caccagcagc aagacccaga aggaccaggu caaggaggac      780

aagaagcgcc ugaaggccuu cgccaaccuc gugcugggcc ugaacgccuc ccucaucgac      840

cuguucggca gcguggagga gcuggaggag gaccucaaga agcugcagau caccggggac      900

acguacgacg acaagcggga cgagcuggcc aaggcguggu ccgacgagau cuacaucauc      960

gacgacugca agagcgucua cgacgccauc auccuccugu ccaucaagga gcccggccug     1020

accaucagcg aguccaaggu gaaggccuuc aacaagcaca aggacgaccu cgccauccuc     1080

aagagccugc ugaaguccga ccgcagcauc uacaacacca uguucaaggu ggacgagaag     1140

gggcugcaca acuacgucca cuacaucaag cagggccgga ccgaggagac guccugcaac     1200

cgcgaggacu ucuacaagua caccaagaag aucguggagg gccugagcga cuccaaggac     1260

aaggaguaca uccucagcca gaucgagcug cagauccugc ucccccugca gcggaucaag     1320

gacaacgggg ugaucccgua ccagcugcac cucgaggagc ugaaggccau ccuggcgaag     1380

ugcggcccca aguuccccuu ccugaacgag gucgccgacg gguucuccgu ggccgagaag     1440

cucaucaaga ugcuggaguu ccgcaucccc uacuacgugg gcccgcugaa cacccaccac     1500

aacgucgaca acgggggcuu cgccugggcc gugcgcaagg cgagcggccg ggugaccccc     1560

uggaacuucg acgacaagau cgaccgcgag aaguccgccg ccgccuucau caagaaccuc     1620

acgaacaagu gcaccuaccu gcugggggag gacguccucc ccaagagcuc ccugcuguac     1680

agcgaguuca ugcuccugaa cgagcugaac aacgugcgga ucgacggcaa gccccucgag     1740

aaggucguga aggagcaccu gaucgaggcg guguucaagc aggaccacaa gaagaugacc     1800

aagaaccgca ucgagcaguu ccugaaggac aacggguaca ucagcgagac ccacaagcac     1860

gagaucacgg gccucgacgg ggagaucaag aacgaccugg ccuccuaccg ggacaugguc     1920

cgcauccugg gcgacggcuu cgaccggagc auggccgagg agaucaucac cgacaucacc     1980

aucuucgggg aguccaagaa gaugcuccgc gagacccugc ggaagaaguu cgccagcugc     2040

cuggacgacg aggccaucaa gaagcucacg aagcugcgcu accgggacug gggccgccug     2100

ucccagaagc uccugaacgg gaucgagggc ugcgacaagg cgggggacgg caccccggag     2160

accaucauca uccugaugcg gaacuucagc uacaaccuca uggagcugcu gggcgacaag     2220

uucuccuuca uggagcgcau ccaggagauc aacgccaagc ucaccgaggg gcagaucgug     2280

aacccccacg acaucaucga cgaccuggcc cugagccccg ccgugaagcg ggccgucugg     2340

caggcgcucc gcaucgugga cgagguggcc cacaucaaga aggcccugcc cgcccggauc     2400

uucgucgagg ugacgcgcuc caacaagaac gagaagaaga agaaggacag ccggcagaag     2460

cgccuguccg accucuacgc cgcgaucaag aaggacgacg ugcugcugaa cggccucaac     2520

aacgagaucu ucggggagcu caagagcucc cuggccaagu acgacgacgc cgcccugcgc     2580

agcaagaagc uguaccugua cuacacccag augggccggu gcgcguacac cggcgagauc     2640

aucgagcucu cccugcugaa caccgacaac uacgacaucg accacaucua cccgcgcagc     2700

cucaccaagg acgacuccuu cgacaaccug guccugugca agcggacggc caacgcccag     2760

aaguccgacg ccuaccccau cagcgaggag auccagaaga cccagaagcc cuucuggacc     2820

uuccucaagc agcaggggcu gaucagcgag cgcaaguacg agcggcugac ccgcaucacg     2880

ccccucaccg ccgacgaccu gagcggcuuc aucgcgcggc agcuggugga gaccaaccag     2940

uccgugaagg ccgccaccac gcuccugcgc cggcuguacc cgggggucga cgugguguuc     3000

gucaaggccg agaacgugac cgacuuccgc cacgacaaca acuucaucaa ggugcggagc     3060

cucaaccacc accaccacgc caaggacgcg uaccugaaca ucgucguggg caacguguac     3120

cacgagcgcu ucacccggaa cuuccgcgcc uucuucaaga agaacggggc caaccggacc     3180

uacaaccugg ccaagauguu caacuacgac gucaacugca cgaacgccaa ggacggcaag     3240

gcgugggacg ugaagaccuc cauggacacc gugaagaaga ugauggacag caacgacguc     3300

cgcgugacca agcggcuccu ggagcagacg ggcgcccugg ccgacgccac caucuacaag     3360

gcgaccgugg ccgggaaggc caaggacggc gccuacaucg ggaugaagac caaguccagc     3420

gucuucgccg acguguccaa guacggcggg augacgaaga ucaagaacgc guacagcauc     3480

aucgugcagu acaccggcaa gaagggcgag gucaucaagg agaucgugcc ccuccccauc     3540

uaccugacca accgcaacac cacggaccag gaccugauca acuacguggc cuccaucauc     3600

ccccaggcca aggacaucag caucaucuac gggaagcucu gcaucaacca gcuggucaag     3660

gugaacggcu ucuacuacua ccucgggggc aagaccaacu ccaaguucug caucgacaac     3720

gccauccagg ugaucgucag caacgagugg aucccguacc ugaaggugcu ggagaaguuc     3780

aacaacaugc ggaaggacaa caaggaccug aaggccaacg uggucuccac ccgcgcgcuc     3840

gacaacaagc acaccaucga ggugcggauc guggaggaga agaacaucga guucuucgac     3900

uaccugguca gcaagcugaa gaugcccauc uaccagaaga ugaaggggaa caaggccgcc     3960

gagcucuccg agaagggcua cggccuguuc aagaagauga gccuggagga gcaguccauc     4020

caccucaucg agcugcugaa ccuccugacg aaccagaaga ccaccuucga ggugaagccc     4080

cuggggauca ccgccagccg cagcacggug ggcuccaaga ucagcaacca ggacgaguuc     4140

aaggucauca acgaguccau caccgggcuc uacagcaacg aggugaccau cguguga        4197


<210>  2123
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2123
auggagaagu ccuacagcau cggccuggac aucgggacca acagcguggg cugguccguc       60

aucaccgacg acuacaaggu gcccgccaag aagaugcgcg ugcucgggaa cacggacaag      120

aaguacauca agaagaaccu gcugggcgcc cuccuguucg acagcgggga gaccgcggag      180

gccacccggc ugaagcgcac cgcccggcgc cgguacacgc gccggaagaa ccgccuccgg      240

uaccugcagg agaucuucgc caaggagaug gccaaggucg acgaguccuu cuuccagcgc      300

cuggacgaga gcuuccucac cgacgacgac aagaccuucg acucccaccc gaucuucggc      360

aacaaggcgg aggaggacgc cuaccaccag aaguucccca ccaucuacca ccuccggaag      420

caccuggccg acagccagga gaaggccgac cuccgccuga ucuaccuggc gcucgcccac      480

augaucaagu uccggggcca cuuccuguac gacaacuuca acgacgacaa cuucgacugg      540

cgcaacaucg acauccagaa gcgguacgag gaguucaucg agacguacga cuccacccug      600

ggggagagcu accucgccga caucuccgug gacgccgcca gcauccugga ggagaaggug      660

uccaagaccg agcgccugga gaaccuccug aaguacuacc ccaccgagaa gaagacgacc      720

uucuucggca accugaucaa gcucauccug gggcagcagg cgaaguucaa ggccaucuuc      780

aaccuggagg acgagaucag ccuccaguuc gccaccccca ccuacgacga ggaccuggag      840

gagcugcucg gcaagaucga caacggggac uccuacagcg agcuguucgu cgccgcccag      900

aaccuguaca acacgauccu ccuggcgucc uuccugaaga ccgacaacaa gagcgccaag      960

gccccgcucu ccaccagcau gaucgagcgg uacgagaacc acaagaagga ccuggccaag     1020

cugaaggacu ucgugaagaa gaacugcccc gaccaguacc acgacaucuu ccgcgacaag     1080

agcaagaacg gcuacgccgg cuacaucgac aacggggugu cccagaacga cuucuacacc     1140

uucaucggca agugccucga ggagagccug aagaaggaca agggggcgca guacuuccug     1200

gacaagaucg accgggacga cuuccuccgc aagcagcgga cguucgacaa cggcgccuuc     1260

cccuaccaga uccaccucca ggagaugcac gccauccugc gccgccaggg cgacuacuac     1320

cccuuccuca aggagaacca ggacaagauc gagaagaucc ugaccuuccg gaucccguac     1380

uacgucgggc cccuggcccg caaggacucc cgguucgccu gggcgaacua ccgcagcgac     1440

gaggccauca cccccuggaa cuucgacgag gugguggaca aggagaaguc cgccgagaag     1500

uucaucaccc ggaugacgcu caacgaccug uaccugcccg aggagaaggu ccugccgaag     1560

cacagccuca ucuacgagac cuucaccgug uacaacgagc ugaccaacau caaguacgug     1620

aacgaccagg gcaacgccau ccacuucgac uccgagcuga aggagaagau cuucaaccag     1680

cuguucaagg agaaccgcaa ggucagcaag aagacgcuga ucgacuuccu gaacaacucc     1740

gaggggaucu acaccgacaa gcucgugggc aucgacgagg aggugaagua ccugaacgcg     1800

agccugggga ccuaccacga ccucaagaag auccuggagu ccuucaugga cgacgagauc     1860

aacgagaaga ucaucgagga caucauccag acccugacgc uguucgagga caucgagaug     1920

aagcggcagc gccugcagaa guacgacgac aucuucaccc ccaagcagcu gaaggagcuc     1980

gcccggcgca acuacaccgg cuggggccgg cugagcuaca agcugaucaa cgggauccgc     2040

aacaaggaga acaacaagac cauccucgac uaccugaaga acggcaaccg gaacuucaug     2100

cagcugauca acgacgaccg ccucuccuuc aagcagauca ucaucgacgc ccggaagauc     2160

gagaagcugg acaacaucga gagcgucgug uacaaccugc ccgggucccc cgccaucaag     2220

aagggcaucc uccagagcau caagaucgug gacgagcugg ucaaggugau ggggcacaac     2280

ccggacaaca ucgugaucga gauggcccgc gagaaccaga cgaccaacca gggcaagaac     2340

cggucccagc agcgccugaa gcggcuccag gacagcaugu ccaacuucaa ggacagcagc     2400

aucucccuga aggacgucga caacagcgac cugcagaacg accgccucuu ccuguacuac     2460

auccagaacg gcaaggacau guacaccggg gaggagcugg acaucgacca ccucuccgac     2520

uacgacaucg accacaucau cccccagagc uucaucaagg acaacuccau cgacaaccgc     2580

gugcugacca gcuccgcgaa gaaccggggc aagagcgacg acguccccgg gcgcgacgug     2640

gugcugaaga ugaagcccuu cuggaagaag cucuacgacg ucaagcugau cuccaagcgg     2700

aaguucgaca accugaccaa gagcgagcac ggcgggcuca cggaguccga caaggccggc     2760

uucaucaagc gccagcuggu ggagacccgg cagaucacca aguacguggc ccagauccug     2820

gacggccgcu ucaacaccaa gcgggacgac aacaacaagg ucauccgcga cgugaaggug     2880

aucacgcuca agagcucccu ggucagccag uuccggaagg acuucggguu cuacaaggug     2940

cgcgagauca acgacuacca ccacgcccac gacgccuacc ugaacgcggu ggucggcacc     3000

gccauccuca agaaguaccc gaagcuggcc cccgaguucg uguacgggga guacaagaag     3060

ugcgacgugc ggaagcugau cgccaagucc ggcgacaaga gcgagaucgg caaggccacc     3120

gcgaaguacu ucuucuacuc caaccucaug aacuucuuca agcgcgucau ccgguacagc     3180

aacgggauga ucguggugcg ccccgucauc gaguacagca aggacaccgg cgagaucgcc     3240

ugggacaagg agaaggacuu caagacggug ugcaaggugc uguccugccc ccaggucaac     3300

aucgugaaga agguggagaa gcagagccac gggcuggacc ggggcaagcc gaagggguuc     3360

uacaacgcca accccucccc caagcccaag aagggcagca aggucaaccu cgugccgauc     3420

aaggccaacc ugaaccccaa gaacuacggc ggguacgcgg gcaucuccaa cagcuacgcc     3480

gugcuggucg acgccaccau cgagaagggg gccaagaaga agcucacccg cauccaggag     3540

uuccagggca ucuccaucau cgaccgggag aaguacgaga agaacaaggu ggaguuccug     3600

aaggggcugg gcuacaagga gaucuacagc aucaucaccc uccccaagua cucccuguuc     3660

gagcuggccg acggcagccg ccggaugcuc gcguccaucc ugagcacgaa caacaagcgc     3720

ggggagaucc acaagggcaa cgagcuggug cucccggcca aguacauccc ccugcuguac     3780

cacgccaacc ggauccacaa caccuucgag accgggcacc gcgaguacgu cgagaagcac     3840

aucgccgagu ucaaggagau cgccgagauc auccucgagu ucaacaacaa guacgugaac     3900

gcgaagaaga acuccagcau caucgagaag gcccuggagu ccuucgacag cuucucccug     3960

gacgagaucu gcgacagcuu cgugggcaag cucaagaaga acaacaccaa gaagaacucc     4020

gggcuguucg agcuggucag ccucggcagc gccuccgacu ucgaguuccu ggagacgaag     4080

gugccccgcu accgggacua cacccccagc ucccugcuca acgccacccu gauccaccag     4140

agcaucaccg gccuguacga gacgcgcauc gaccucucca agcuggggga ggaguga        4197


<210>  2124
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2124
auggagaccc aggccagcaa ccagcugauc uccagccacc ucaagggcua ccccaucaag       60

gacuacuucg uggggcugga caucggcacc uccagcgucg ggugggccgu gacgaacaag      120

gcguacgagc ugcucaaguu ccgcucccac aagauguggg gcagccggcu guucgacgag      180

ggggaguccg ccguggcccg ccggggcuuc cgcagcaugc ggcgccggcu ggagcgccgg      240

aagcuccgcc ugaagcugcu cgaggagcug uucgccgacg ccauggcgca ggucgacccg      300

accuucuuca ugcggcugcg cgaguccaag uaccacuacg aggacaagac caccggccac      360

agcuccaagc acauccucuu caucgacaag aacuacacgg accaggacua cuacaaggcc      420

uaccccacca ucuaccaccu ccgggccgag cugaugaagg acgggaccua cgacauccgc      480

aagcuguucc uggccgugca ccacauccug aaguaccggg gcaacuuccu cuacgagggg      540

gcgagcuucg acuccaacgc cagcacccug gacgacgugc ugaagcaggc ccucgagaac      600

aucacguuca acugcuucga cuccagcgcc gccaucggcu ccaucgggca gauccugaug      660

gagaccggca agaccaagag cgacaaggcg aagaccaucg agcgccuggu cgacacguac      720

aucaucaccg acgaggugaa cgccuccggc aagacccaga aggaccaggu gaaggaggac      780

aagaagcggc ucaaggccuu cgccaaccug guccuggggc ucaacgccag ccugaucgac      840

cuguucggca gcguggagga gcucgaggag gaccugaaga agcugcagau caccggggac      900

acguacgacg acaagcgcga cgagcucgcg aaggccuggu ccgacgagau cuacaucauc      960

gacgacugca agagcgugua cgacgccauc auccugcugu ccaucaagga gcccggccuc     1020

accaucagcg aguccaaggu caaggccuuc aacaagcaca aggacgaccu ggccauccuc     1080

aagagccugc ugaaguccga ccggagcauc uacaacgcga uguucaaggu ggacgagaag     1140

ggccugcaca acuacgugca cuacaucaag caggggcgca ccgaggagac cuccugcaac     1200

cgggaggacu ucuacaagua cacgaagaag gucguggagg gccuccccga cagcaaggac     1260

aaggaguaca uccuguccga gaucgagcug cagacccucc ugccgcugca gcgcaucaag     1320

gacaacgggg ugauccccua ccagcuccac cuggaggagc ugaaggucau ccuggccaag     1380

ugcggcccca aguuccccuu ccucaacgag guggccgacg gguucagcgu ggccgagaag     1440

cugaucaaga ugcuggaguu ccgcaucccg uacuacgucg gcccccucaa caccucccac     1500

aacguggaca acggcggguu cgccugggcg gugcggaagg ccagcggccg cgucaccccc     1560

uggaacuucg acgacaagau cgaccgggag aaguccgccg ccgcguucau caagaaccug     1620

acgaacaagu gcaccuaccu gcucggggag gacgugcugc ccaagagcuc ccugcucuac     1680

agcgaguuca ugcugcugaa cgagcucaac aacguccgca ucgacggcaa gccgcuggag     1740

accaagguga aggcccaccu gaucaaggcc guguucaagc aggaccacaa gaagaugacc     1800

aagaaccgga ucgagcaguu ccucaaggac aacaacuaca uccccgagaa gcacaagccc     1860

gagaucacgg ggcuggacgg cgagaucaag aacgaccugg ccuccuaccg cgacaugguc     1920

cggauccucg gcaacaacuu cgaccugagc auggccgagg agaucaucac cgacaucacc     1980

aucuucgggg agagcaagaa gaugcugcgc gagacccucc ggaagaaguu cgcguccugc     2040

cuggacgacg aggccaucaa gaagcugagc aagcuccgcu accgggacug gggccgccug     2100

uccaagaagc ugcucaacgg gaucgacggc ugcgacaagg ccggggacgg cacgagcgug     2160

accaucaucg aggccaugcg ggacuucucc uacaaccuga uggagcugcu cggcgaccgc     2220

uuccuguuca uggagcggau ccaggagauc aacgacaagc ugaccgaggg gcagaucgug     2280

aacccccacg acaucaucga cgaccucgcc cugagcccgg cggucaagcg cgccgugugg     2340

caggcccugc ggaucgugga cgagaucguc cacaucaaga aggcccuccc cgcccgcauc     2400

uucguggagg ugacccgguc caacaaggcg gagaagaaga agaaggacag ccgccagaag     2460

cgccuguccg accuguacgc cgccaucaag aaggacgacg cccuccugag cggccugacg     2520

aacgaggagu ucgggggccu caaguccgag cuggcgaagu acgacgacgc cgcccugcgg     2580

agcaagaagc ucuaccugua cuacacccag augggccgcu gcgccuacac cggggagguc     2640

aucgaccugu cccagcucaa caccgacaac uacgacaucg accacaucua cccccggagc     2700

cugaccaagg acgacuccuu cgacaaccug gugcucugca agcgcacggc caacgcgcag     2760

aaguccgaca ccuaccccau cagcaaggag auccagaagg cccagaaggc cuucuggauc     2820

uuccugaagc agcagggccu gaucagcgag cggaaguacg agcgccucac ccggaucacc     2880

ccgcugacgg ccgacgaccu gagcggguuc aucgcccgcc agcucgugga gaccaaccag     2940

uccgucaagg cggccaccac ccugcugcgg cgccucuacc ccgacaucga cgugguguuc     3000

gucaaggccg agaacgugac ggacuuccgg cacgacaaca acuucaucaa ggugcgcagc     3060

cugaaccacc accaccacgc caaggacgcc uaccugaaca ucgucguggg caacguguac     3120

cacgagaagu ucacccggaa cuuccgcgcg uucuucaaga agaacggggc caaccggacc     3180

uacaaccucg ccaagauguu caacuacgac gucaagugca ccaacgccaa ggacggcaag     3240

gcgugggacg ugaagacguc cauggacacc gugaacaaga ugaugaccag caacgacguc     3300

cgcgugacca agcggcugcu ggagcagucc ggcgcccucg ccgacgccac gguguacaag     3360

gccaccgucg cggccaaggc caaggacggg gccuacaucg gcaugaagac caagagcucc     3420

guguucgccg acgugaccaa guacgggggc augacgaaga ucaagaacgc guacagcauc     3480

aucguccagu acaucgggaa gaagggcgag guggugaagg agaucguccc ccugcccauc     3540

uaccugacca accgcaacau caccgaccag gaccucaucg acuacguggc cuccaucacc     3600

ccgcaggcca aggacaucag caucaucuac cggaagcugu gcaucaacca gcuggugaag     3660

gucaacggcu ucuacuacua ccucgggggc aagacgaacu ccaacaucua caucgacaac     3720

gccguggagg uggucgugcc cgccaacauc gcgguguaca ucaagguccu ggagaaguuc     3780

gcccugcucc acaaggacaa caacaacauc aaggccagca ccgugaccac ccgcaucuac     3840

aacgaggggu ccacgaacau cgugagcauc accgacaagg acggccugau cgucuacgac     3900

uaccugaugu ccaagcuccg gacgccccug uacaugaaga ugaaggggaa caagguggac     3960

gagcugagca ccgugggccg cuccaaguuc augaacauga cccucgagga gcagagcauc     4020

uaccugcugg agguccucaa ccugcugacc aacuccaaga ccaccuucga cgugaaggcc     4080

cucggcauca cggccagccg cagcaccauc ggggucaaga uccacacccu ggacgaguuc     4140

aagaucauca accaguccau caccggccug uacagcaacg agaucaccau cguguga        4197


<210>  2125
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2125
auggagaccc agaccagcaa ccagcugauc acgucccacc ucaaggacua ccccaagcag       60

gacuacuucg ugggccugga caucgggacc aacagcgucg gcugggccgu gaccaacacc      120

uccuacgagc ugcucaaguu ccacagccac aagauguggg ggucccgccu guucgaggag      180

ggcgagagcg ccgugacgcg gcgcggguuc cgguccaugc gccggcgccu ggagcggcgc      240

aagcuccggc ugaagcugcu cgaggagcug uucgcggacg ccauggccca ggucgacagc      300

accuucuuca uccgccugca cgaguccaag uaccacuacg aggacaagac caccggccac      360

agcuccaagc acauccucuu caucgacgag gacuacacgg accaggacua cuucaccgag      420

uacccgacca ucuaccaccu ccggaaggac cugauggaga acggcaccga cgacauccgc      480

aagcuguucc uggccgugca ccacauccug aaguaccggg ggaacuuccu cuacgagggc      540

gccacguuca acagcaacgc guucaccuuc gaggacgugc ugaagcaggc ccuggucaac      600

aucaccuuca acugcuucga caccaacucc gccaucagcu ccaucagcaa cauccucaug      660

gaguccggga agaccaagag cgacaaggcc aaggcgaucg agcgccuggu ggacaccuac      720

accguguucg acgaggucaa cacccccgac aagccccaga aggagcaggu caaggaggac      780

aagaagacgc ugaaggccuu cgccaaccuc gugcugggcc uguccgccaa ccucaucgac      840

cuguucggga gcguggagga caucgacgac gaccugaaga agcuccagau cgugggcgac      900

accuacgacg agaagcggga cgagcuggcc aagguguggg gcgacgagau ccacaucauc      960

gacgacugca agagcgucua cgacgcgauc auccugaugu ccaucaagga gcccgggcuc     1020

accaucagcc aguccaaggu gaaggccuuc gacaagcaca aggaggaccu ggugauccuc     1080

aagagccucc ugaagcugga ccgcaacguc uacaacgaga uguucaaguc cgacaagaag     1140

ggccuccaca acuacgugca cuacaucaag caggggcgga ccgaggagac gagcugcucc     1200

cgcgaggacu ucuacaagua caccaagaag aucguggagg gccuggccga cagcaaggac     1260

aaggaguaca uccugaacga gaucgagcuc cagacccugc ugccgcucca gcggaucaag     1320

gacaacggcg ucauccccua ccagcugcac cuggaggagc ucaaggugau ccuggacaag     1380

ugcgggccca aguuccccuu ccugcacacc guguccgacg gcuucagcgu cacggagaag     1440

cugaucaaga ugcucgaguu ccgcaucccg uacuacgugg ggccccugaa cacccaccac     1500

aacaucgaca acggcggguu cuccugggcc guccggaagc aggccggccg cgugaccccc     1560

uggaacuucg aggagaagau cgaccgcgag aaguccgcgg ccgccuucau caagaaccug     1620

accaacaagu gcacguaccu cuucggcgag gacgugcugc ccaagagcag ccugcucuac     1680

uccgaguuca ugcugcugaa cgagcucaac aacguccgga ucgacgggaa ggcccuggcc     1740

cagggcguga agcagcaccu gaucgacagc aucuucaagc aggaccacaa gaagaugacc     1800

aagaaccgca ucgagcuguu ccugaaggac aacaacuaca ucaccaagaa gcacaagccg     1860

gagaucaccg ggcuggacgg cgagaucaag aacgaccuca cguccuaccg ggacauggug     1920

cgcauccugg ggaacaacuu cgacgucagc auggcggagg acaucaucac cgacaucacc     1980

aucuucggcg aguccaagaa gaugcugcgg cagacccucc gcaacaaguu cggcagccag     2040

cugaacgacg agaccaucaa gaagcugagc aagcuccggu accgcgacug ggggcggcug     2100

uccaagaagc ugcucaaggg caucgacggg ugcgacaagg ccggcaacgg ggcccccaag     2160

acgaucaucg agcugaugcg caacgacagc uacaaccuga uggagauccu cggcgacaag     2220

uucuccuuca uggagugcau cgaggaggag aacgccaagc uggcccaggg ccagguggug     2280

aacccccacg acaucaucga cgagcuggcg cucagccccg ccgucaagcg ggccgugugg     2340

caggcccugc gcaucgugga cgaggucgcg cacaucaaga aggcccugcc gucccggauc     2400

uucguggagg uggcccgcac caacaagagc gagaagaaga agaaggacuc ccggcagaag     2460

cgccucagcg accuguacuc cgccaucaag aaggacgacg uccugcagag cgggcuccag     2520

gacaaggagu ucggcgcccu gaaguccggg cuggcgaacu acgacgacgc cgcccuccgg     2580

agcaagaagc uguaccugua cuacacccag augggccgcu gcgccuacac cggcaacauc     2640

aucgaccuca accagcugaa cacggacaac uacgacaucg accacaucua cccccggucc     2700

cugaccaagg acgacagcuu cgacaaccuc gugcugugcg agcgcaccgc caacgcgaag     2760

aaguccgaca ucuaccccau cgacaaccgc auccagacca agcagaagcc cuucugggcc     2820

uuccugaagc accaggggcu caucagcgag cggaaguacg agcgccugac gcggaucgcc     2880

ccgcugaccg ccgacgaccu cuccggcuuc aucgcccgcc agcuggugga gaccaaccag     2940

agcgucaagg cgaccacgac ccugcuccgg cgccuguacc ccgacaucga cgugguguuc     3000

gucaaggccg agaacgugag cgacuuccgg cacaacaaca acuucaucaa ggugcgcucc     3060

cugaaccacc accaccacgc caaggacgcc uaccucaaca ucgucguggg gaacguguac     3120

cacgagaagu ucacccggaa cuuccgccug uucuucaaga agaacggcgc gaaccggacc     3180

uacaaccugg ccaagauguu caacuacgac gucaucugca cgaacgccca ggacgggaag     3240

gccugggacg ugaagaccag caugaacacc gugaagaaga ugauggccuc caacgacguc     3300

cgcgugaccc ggcgccuccu ggagcagagc ggcgcgcugg ccgacgccac gaucuacaag     3360

gccuccgugg ccgcgaaggc caaggacggc gccuacaucg ggaugaagac caaguacagc     3420

gucuucgccg acgugaccaa guacggcggg augaccaaga ucaagaacgc cuacuccauc     3480

aucgugcagu acacgggcaa gaagggggag gagaucaagg agaucguccc ccuccccauc     3540

uaccugauca accggaacgc gaccgacauc gagcugaucg acuacgucaa gagcgugauc     3600

ccgaaggcca aggacaucuc caucaaguac cgcaagcucu gcaucaacca gcuggugaag     3660

gugaacggcu ucuacuacua ccugggcggg aagaccaacg acaagaucua caucgacaac     3720

gccaucgagc ucgugguccc ccacgacauc gccaccuaca ucaagcugcu ggacaaguac     3780

gaccuccugc ggaaggagaa caagacgcug aaggcgagcu ccaucaccac cagcaucuac     3840

aacaucaaca ccuccacggu ggugagccuc uccaacaagg ucggcaucga cguguucgac     3900

uacuucauga gcaagcugcg cacgccccug uacaugaaga ugaaggggaa caagguggac     3960

gagcucucca gcaccggccg guccaaguuc aucaagauga cccuggagga gcagagcauc     4020

uaccugcucg agguccugaa ccugcucacg aacagcaaga ccaccuucga cgugaagccc     4080

cuggggauca ccggcucccg cagcaccauc ggcgugaaga uccacaaccu ggacgaguuc     4140

aagaucauca acgaguccau cacggggcuc uacagcaacg aggucaccau cguguga        4197


<210>  2126
<211>  4200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2126
augaagacca uccugggccu cgaccugggg accacgagca ucggcugggc ccuggugaag       60

gaggccgagc ccuucuccga cgagaagucc gagaucgucc gccucggggu gcgggugauc      120

ccgcugaccg ucgacgagcg caccagcuuc gagaacggca agagcaucac cacgaacgcg      180

cugcggaccc agaagcgcgg gaugcggcgc aaccuccagc gguacaagcu gcgccggcag      240

gcccugaucg aggagcucaa guccaccggc uggaucggca acgacuucau cuucagcgag      300

accgggcccg gcuccacguu ccgcacccug cggcugcgcg ccaagagcgc cuccgaggag      360

gugacccucg gggaccuggc ccggauccug cuccagauca acaagaagcg cggcuacaag      420

agcucccgga agauggugag cuccgacgac gggcaggcga ucgacggcau ggccgucgcc      480

aaggagaugu acgacaaggg ccugaccccc ggggaguacg uguaccagcg caugcuggag      540

ggcaaguacg ccauccccga guucuacaag agcgaccucc aggaggaguu cgaccggauc      600

ugggcgcugc agaaguccuu ccacaucgag cugacggacg agcucuaccg caagcugagc      660

gaccggaaca agucccagac cugggccauc ugccgcgacg agcuggggau ccagggccuc      720

aagagcuccu ucaucggcaa ggagcggcug aaggaggccu accgcuaccg ccaccuggcc      780

gccacccagg aggugaacau ggagguccug accugcgcgc uccaggagau caacgggcag      840

aaggccagcc ugagcggccu ccuguccaag aucagcgacc gguccaagga gcuguaccuc      900

aacaaccuga cgguggggca gaugcugcuc aagcgcaucg aggacaaccc gaacaacagc      960

cugaagaaca ucguguucua ccggcaggac uaccuggacg aguucgagcg caucuggacc     1020

guccagucca aguaccacac cgagcucacc cacgagcuga agaagcggcu gcgcgacauc     1080

aucaucuucu accagcggcc ccucaagagc cagaagggcc ugaucucccu gugcgagcuc     1140

gagacgaagg acaucgaggu ggccaucgac ggggugaaga agaugaagaa caucggcccc     1200

cgcgucugcc cccggagcuc cccgcuguuc caggaguuca agaucuggca gaccaucaac     1260

aacaucuucg ugaacggcgc cgcccuggac gacgaggaca agagcgugau cgcgaaggag     1320

cucauguuca aggacaagcu gaaggacucc gaccugcuga agcuccuguu caagaccacc     1380

aaggggcaga gcgucaacuu caaggaggug gacggcaacc gcacgauggc cacccugaug     1440

aaggccugcu ucaagaucgc cgagaaccuc ggguacgacg acaucggccu guccaagcug     1500

agcuccgagg accagauggu gcggaucgag gggaucuuca aggagcaggg cuucaagacc     1560

ggcuuccucc acuucgacag cucccuggcc agccccgagu ucgagaacca ggacuccuac     1620

cgccuguggc accuccugua cagcuacgag ggggacaaga gcgcguccgg caacgagagc     1680

cuggucaagu gcaucaugga ccucugcucc auggacgagg ggagcgccaa gauccuggcc     1740

uccugcaccu uccugcccga cuacggcagc cucuccgcca aggccaugcg gaagauccug     1800

cccuacauga aggacgggcu ggaguacacg gcggccugcg ccauggccgg cuaccgccac     1860

agcaagcggu cccucaccaa ggaggagcug gacaccaagg aguacagcga ccgccugucc     1920

cuccugccga agaacagccu gcggaacccc gugguggaga agauccucaa ccagaugauc     1980

aacgucguga acuccgucau cgacguguac ggcaagcccg acgagauccg caucgagaug     2040

gcgcgggagc ugaagaaguc cgccaaggag cgcgaggagg cccagcgcga caucgccagc     2100

agcaccgccg agucccagaa ggugaucgag auccugaagg gggagcccuu cuuccuccag     2160

uucccgacgc ggaacgacau cauccgcuac cggcuguacc aggagcuggc gcagaacggc     2220

uucaagaccc ucuacagcaa caccuacauc cccaaggaca agcuguucag caaggaguuc     2280

gacaucgagc acaucauccc ccaggcccug aucuucaacg acuccuucgc caacaagacc     2340

cucgagagcc gccagugcaa ccuggagaag gggaaggaga cggccaugga cuacgucaag     2400

uccaaguacg acgagaccgg cgugcagacc uacugcgacc gggugaacgg ccugaucggg     2460

aagggcauca gcaagaccaa ggccaagugg cuccugaugg aggggaagga cauccccgag     2520

gacuucauca accgcgagcu gcgggacucc caguacaucg cgaagaaggc ccgcgagauc     2580

cucgagagcc uggucaaguu cguggugccg accacgggcu ccgucaccgc ccggcugcgc     2640

gaggacuggc agcucgugaa cgugaugcag gagcugaacu gggagaagua cgcccggcug     2700

gggcucaccg gcguccgcga ggacaaggac ggccggcgca uccccuucau ccaggacugg     2760

accaagcgga acgaccaccg ccaccacgcc auggacgcgc ugacgaucgc cuucacccgg     2820

cgccaguuca uccaguaccu gaaccaccuc aacagcaaga ucgacguggu guccugggac     2880

aagaaggacc uggaccugcg ggacuacgac cucgaggaca ucaaguucgg gaaccugagc     2940

gccggcgacc gcuacgggau cgucaaggcc cugcaggaca aguuccucua caaggacggc     3000

aacgacaagu accgguucgu gccgcccauc ccccuggacg aguuccgccg ccaggcgaag     3060

gagcagcugu ccgacauccu caucagcuuc aaggccaaga acaaggugug cacccggaac     3120

gucaacguga ccaagaacaa gggcggggcc aaccgcaagg cccagcugac gccccggggc     3180

ccccugcaca acgagaccau cuacgggucc agccucgagu acgugaccaa ggagaacgag     3240

aagaucggcu ccagcuucga caccgagcgc aucacgaccg ucugcaagaa gaaguuccgg     3300

gacgcccugg cgcgccggcu ggaggaguuc gggggcgacc ccaagaaggc cuucaccggg     3360

aagaacuccc cggagaagaa ccccaucugg guggacgagc accacagcga gcaggucccc     3420

gccaaggugc gcaccgugac gaugggccag cgguucaccg gccgcaagcc gaucgacgcc     3480

acccucaaga ucgagaaggu gaucgacaag cggauccgcg agauccugca ggcccggcug     3540

gacgaguucg acgggaaggc ggccaaggcc uucuccaacc ucgacgagaa ccccaucugg     3600

cugaacaagg agaagggcau cgccaucaag cgcgucaccg ugagcgggcc cgccaacccc     3660

gugccggucc gguucaagcg cgacaaggac ggcaagccca ucaucgacga cgcggggaag     3720

acgaucggcg ccgacuucgu gacccccggc aacaaccacc acaucgccau cuuccgggac     3780

agcuccggga agcugcagga gcaccccgug agcuuccucg aggccaccau cgcgaagucc     3840

cacggccugg acgucaucga ccgcaacuac aacaaggacg agggguggga guuccuguuc     3900

acccucaagc agaacgagua cuucguguuc ccgaacagcg agacgggcuu caacccccug     3960

gacuacgacc ugaccgaccc ccggaacuac gccgagaucu cccccaaccu cuaccgcgug     4020

cagagcaucu ccaccaacga cuacuucuuc cgccaccacc uggagaccac gagcgagaag     4080

aacaacuccc uguacggcau caccuggaag cggauccgca acgccagcgc ccucgagggg     4140

cuggucaagg ugcggaucga caaccugggc cgcaucgugg ccgucgggga guacgacuga     4200


<210>  2127
<211>  4200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2127
auggagaccc aggccaccgc ccagcugauc agcggccacc ucaaggggua ccccaucuac       60

gacuacuucg ugggccugga caucgggacg aacuccgucg gcugggcggu gaccaaccgc      120

aucuacgagc ugcucaaguu ccggagccac aagauguggg ggucccgccu guucgaggag      180

ggccagaccg ccgugguccg gcgcggcuuc cggagcaucc gccggcgccu ggagcggcgc      240

cggcuccgcc ugaagcugcu cgaggcccug uucgccgacg ccauggcgcg gguggacucc      300

accuucuuca uccgccugcg ggagagcaag uaccacuacg aggacaagac gaccgggcac      360

aaguccaagc acauccucuu caucgacgac aacuacaccg accaggacua cuucgccgag      420

uacccgacca ucuaccaccu ccgcgccgac cugauggaga acggcacgag cgacauccgg      480

aagcuguucc uggccgugca ccacauccug aaguaccgcg ggaacuuccu cuacgagggc      540

gcgaccuucg acuccaacag cucccuggac accguccuga agcgggcccu cgagcagauc      600

gaguuccaca ucuucaacgc caccagcgag gacgcccugc acuccauccu ggacaucgug      660

aagcuccagg acaagaccaa gagcgacaag gccaaggcga ucgagucccu gguggacacg      720

uacaucaccg acaaggucga gagcuccugc accgacaagg agaagaagga ccugcucaag      780

gccgacaaga agcgccugcg ggccuucgcc aaccugaucc ucgggcugaa cgccaaccug      840

acggaccucu ucggcaccau cgaggagauc gaggaggacu ucgugaagcu gcagaucacc      900

ggcgacaccu acgaggagaa gcgcgacgac uacgcgaagg uguggggcga cacgauccgc      960

aucaucgacg acugcaagag cgucuacgac uccaucaucc ugaugagcau caaggagccc     1020

gggcucacca ucagccaguc caaggugaag gccuucaaca agcacaagga ggaccuggcc     1080

auccugaagc ggcuccucaa gagcgacaag uacaucuaca agaacauguu caaggccgac     1140

gagaaggggc uggccaacua cgugcacuac aucaagcagg gccgcaagaa cgagaccggc     1200

ugcuccugcg aggacuucua caaguacacc aagaaggucg uggagagccu gcccgacucc     1260

agcgacaagg cguacauccu gaacgagauc gagcugcaga agcuccugcc ccugcagcgg     1320

aucaaggaca acggggugau cccguaccag cuccaccugg aggagcugaa ggccauccug     1380

gagaagugca aggacacguu ccccuuccuc aacaccgucu ccgacggcuu caccguggcc     1440

gagaagcuga ucaagaugcu ggaguuccgc auccccuacu acguggggcc ccucaacccg     1500

agccacucca ucgacaaggg cggguucgcc ugggucgagc ggaaggagcc cggccaggug     1560

acccccugga acuucgagga caagaucgac cgcgagaagu ccgccgaggc guucaucaag     1620

aaccugacga acaagugcac cuaccugauc ggcgaggacg ugcuccccaa gagcagccug     1680

cuguacuccg aguucaugcu ccugaacgag cugaacaacc uccggaucga cgggaagccg     1740

cuggagcagg ccgucaagau gcaccugauc gacgccgugu ucaagaagga gcacaagaag     1800

cagaccaaga accgcaucau cgaguuccuc aaggacaaca acuacauccu gggcaagcac     1860

aagcccgagg ucgacgggcu ggacgagagc aucaagggcg accucgccuc cuaccgggac     1920

augaagcgca uccuggggga cgacuucgac gugagcaugg cggaguccau caucaccagc     1980

aucacgaucu ucggcgaguc caagaacaug cugcgggaga cccuccgcaa gaaguucggc     2040

agcauccuga gcgaggacca gauccggucc cuggccaagc uccgcuaccg ggacuggggg     2100

cgccugagca agaaguuccu gaucggcauc gacgggugcg ugaagggcga ggacgggacc     2160

cccggcacca ucaucgacau gaugcggaac acgucccaca accucaugga gcugcugggc     2220

gacaccuuca gcuucaugga cgucaucauc gaggagaacg acaagcucac caagggccag     2280

gugguggacc cccacgagau caucgaggag cuggggcugu ccccggccgu caagcgcgcc     2340

guguggcagg cccuccggau cguggacgag gucauccaca ucaagaaggc gcugcccagc     2400

cgcaucuucg uggagguggc ccgcaccaac aaggacgaga agaagaagaa ggacucccgg     2460

aagaagcgcc ugcaggagcu cuacaagagc auccagaagg agucccccga guggcugagc     2520

gucacccggg acgaguccga caagcugauc agcaaccucg accgcgccga cgacuccagc     2580

cugcggucca agaagcugua ccucuacuac acgcagaugg ggcgcugcau guacagcggc     2640

cgggugaucg accuggacca gcugaacacc gacaaguacg acaucgacca caucuacccc     2700

cgcucccuca ccaaggacga cagcuucgac aaccuggugc ugugcgagcg gcaggagaac     2760

gccaagaagu ccgacaacua cccgaucgac cccgacaucc agaacgcccg caagcccuuc     2820

uggaccuucc ucaaggacca gaaccugauc agcagccgga aguacgagcg ccugacgcgg     2880

guccuccccc uggaggcgga cgaccugacc ggcuucaucg cccgccagcu cguggagacc     2940

aaccaguccg ugaaggccgu caccacgcug cugcggcgcc ucuacccggg gguggacgug     3000

gucuucguga aggccgagaa cgugaccgac uuccggcacg acaacgacuu caucaagguc     3060

cgcagccuga accaccacca ccacgccaag gacgcguacc ugaacaucgu ggugggcaac     3120

gucuaccacg agaaguucac ccggaacuuc cgcaaguucc ucaagaagaa cggggccaag     3180

cggaccuaca accuggcccg cauguucgac uucgacguga uguccacgaa cgccaaggac     3240

ggcaaggcgu gggacaucaa gaccagcaug aacaucguga agaagaugau ggccuccaac     3300

gacguccgcg ugaccaagcg gcugauggag cagaaggggg cccucagcga cgccaccgug     3360

uacaaggccu ccgucgcggg caaggccaag ggcggggccu acaucggcau gaagacgaag     3420

gacagcgugu ucuccgacgu gagcaaguac gggggccgca ccuccaucaa gaacgccuac     3480

agcaucgucg uggaguacac cgggaagaag aacgagauca ucaaggagau cgugccccug     3540

cccgucuacc ugaucaaccg gaacaagacc gaccaggagc ucauccacua caucgugucc     3600

aucauccccc aggccaagaa cgugagcauc aucuacaaga agcugugcau caaccagcug     3660

gucaagguga acggcuucua cuacuaccuc ggcgggaaga cgaacaacua cuucuggauc     3720

gacuccgcga uccagguggu caucgccgac gaguggaucc cguaccugaa ggugcuggag     3780

aaguucgacg ugaugcgcaa gcagaacaag gagcucaagg ucggcaccgu gagcaccucc     3840

auccagaacc aggggaagaa gguggucauc aagauccugg aggagcggaa cguggaguuc     3900

uucagcuacc uggucggcaa gcuccacagc uccaucuaca gcaagaugaa ggggaacaag     3960

gccggcgagc uggccaccaa gucccuggag aucuucguga acaugagccu cgagaaccag     4020

ugcaucuacc ugauggagcu gcucaaccug cugacgaaca agaaggcccc cgacgugaag     4080

ccccucggca ucaccgcguc ccgcgggacc aucggcagca aggucuccaa ccaggacgag     4140

uucaagauca ucaacgagag caucaccggg cuguacucca acgaggugac gaucguguga     4200


<210>  2128
<211>  4200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2128
augaaccugc gcaacaccac cgacgaguac aacaucggcc ucgacauggg gacgaacagc       60

gugggcuggg ccgucaccga caaggccggg gcgaucgccc acuucaaggg caagcccacc      120

cugggguccc ggcuguucga cgccgcccag accgccgcgg gcgcccgcau ccaccggagc      180

cagcgccggc gcuacgugcg gcgccggugg cgccucgacc ugcugcaguc ccucuuccag      240

gacgagguga gcaaggucga cuccgaguuc uucugccggc ugcgccagag ccggcuguac      300

gugggcgacc gcgagaaggg gcacgccgac uacacguggc cgaucuucaa cgacuccgac      360

uucaacgagg uggacuacua caagcgguuc cccaccaucu accaccuccg caaguggcug      420

auggagaccg acgagaaggc cgacauccgg cugaucuacc ucgcgaccca caacaucguc      480

aagcaccgcg gcaacuuccu gcgggagaac aacaagagcc uguccagccg cgacgccaac      540

cccgcccagg ccgugaaguc ccuccggcug gcccugaacg acuggugcag cgagcgcgac      600

uacgagugcg cguggaaggg ggaggaggac gacggcgcca ucgacgaggc cauccucaac      660

gugcuggccg acggggagaa gggccgcucc gaccaggcca aggagaucgc gaagcugacg      720

ggcgucgacu gcggggacgc caagcagagc aagcagugca acgaggccau cgccaaggcc      780

aucgugggcc ucaaggugga gaugaagaac aucuucgggg aguuccccgc ggagaagacc      840

uccuacaucu accugagcuc cgacgaggac gucgaggccg ugcgggagag cugcccggac      900

gacuccggcg agcuguucca ggcccucugc aagcuguaca gcgccuacau ccugcaggag      960

cuccugagcu acgccgaccc cuccggccag accaucagcg cgaacauggu ggccaaguac     1020

aagcaguacg ggcgcgaccu gcggcuccug aaggagcuga cccgcacgua cgcgccccag     1080

aaguacgacc ucuucuuccg gggcgagacc uacgccgggu ccagcgacua cgucguggac     1140

gaggcccgcg gcuacacccg guacaaccug gggaccacga aggccaacua cgacgacuuc     1200

cagaaguccg ugaaggagcu guucaagggc accgacgccc uccaggacga gcgcuaccag     1260

gagaugaugg ucgccuucgg cgagcagaag uuccugcggc gccugaagac cagcgacaac     1320

ggguccaucu acuaccagcu ccaccuggag gagcugcaca ccauccucga gaaccagggc     1380

aagcacuacc ccuuccugcu gagcgaggag gacaagaucg agucccucgu gagcuuccgg     1440

aucccguacu acguggggcc ccugacgcgc aagaacgccg cgaucggcca cgacgggaag     1500

gagcgguucg ccugguccag ccgcaaggac ggcauggagc acgccgucau cacccccugg     1560

aacugggagg agaucaucga ccgggacaag uccgccgagg acuucaucau gcgcaugacc     1620

ggcgacugca ccuaccugca gggggagccc gugcucccga agugcagccu gcuguacgag     1680

gaguucugcg cccugaacga guucaacggc gcgcacugga cgguggacgg ggacgacgag     1740

cggcgcuucg acgccgccca gcgggagcgc aucaugugcg accucuuccg ccggacccgc     1800

uccgucaccu acaacaaggu gagcgacugg cuggagcgga aguacaacuu caccggcgcc     1860

cgcguccgcg ggggccaggg cgagcacggg uucgagucca agcugggcag cuacaucuuc     1920

uucugcaagg acguguucaa gacggacggg cucgacgagg ccgacuacac caugaucgag     1980

cagaucaucc ugugguccac ccuguucgag gaccgggaca uccucaagcg gaagaucgag     2040

gaggcguacg gcggcgaccg ccugaccagc gagcagauca aggugaucug caagaagcgg     2100

uucacggggu ggggccgccu gagcaagcgg cuccugaccg acaucaaggu caacaccgac     2160

accgggcgca aguccaucau ggacgugcug cgggagggca accccaacaa cgggcagcgc     2220

agccggacga ugguguucau ggagguccuc cgcgacgagg accugggcuu ccagaaggcc     2280

aucgacggcc ugaaccggga cuacuuccgc gagcacaaca acgggcucau ggacgugaac     2340

gaccugcccg gcucccccgc cauccggcgc agcaucaacc aggccgugcg gaucgucgac     2400

gagaucgcgu ccaucgcccg caaggagccg accaacaucu ucaucgaggu gacccgcgag     2460

gaggacgacc cccggaagaa ggggaagcgc accaagcggc gcuacgacgc caucaagagc     2520

gcccuggaga aguucaagaa ggacgacccc ucccucugga aggagcugug cgagaaggcc     2580

cccggcgaca uggacgagcg gcugagccuc uacuucaugc agcgcgggaa gugcauguac     2640

uccggccggc cgaucaagau cgaggagcug cacaccggca aguacgaggu ggaccacauc     2700

aucccccgca gcuacaucaa ggacgacucc uucgagaaca agguccuggu guaccgggag     2760

gagaaccagc acaagacgga cgcgcuccug aucgacagcu ccauccgcca gaagaugggc     2820

ggguacuggc ggaugcugca cgacgccaag cucguggggg acaagaaguu ccgcaaccug     2880

uuccggaacc acaucgacga caaggccaug aagggcuucg ucgcccgcca gaucguggag     2940

accagccaga uggugaagcu gguccagacc cucuucgagg cccgguaccc caacacccgc     3000

aucgugcccg ugaaggcggg gaucucccac gaccugcggg aggccgccgg ccuggucaag     3060

ugccgcgagg ccaacgacuu ccaccacgcc cacgacgcgu uccucgccug ccggaucggc     3120

cuguucaucc agaagcgcca cccgagcgug uacgacaacc ccaucgggcu ggcccgcauc     3180

augcgggacu acgcccgcau gcaggcgcgg gaguacaaga agucccacug ccucgcccac     3240

acgcagggcu ucaucgugaa cagcuuccug agcuccgggu ucgacgagga gaccggcgag     3300

aucuucaagg acgacuggga cgccaaggcc gaggucgagg ggauccgccg ggugcugaac     3360

ugccgccagu gcuucaucag ccggaugccc guggagggcg acacccgcua cggcgggcgg     3420

uacuggggcg agaccaucua cucccccaag gggaagcaca agcacgagau cccgcucaag     3480

cagggccugg accccaaccu guacggcggg uacagcgacc uccuguacuc cuacuuccug     3540

aucuacgagg ccagcgacgu cuccagcggc gagcgcgugc uccgguucau cgggaucccc     3600

acguccaucg cggcccgcau ggccaaccag aagcggcugg aggaguacgu gagccgccug     3660

gcccuccagg agaaccugga gcacguccgg auggugcgca agaucaugaa gaagcagcug     3720

aucgaggugg acaacugccg gcuccugguc accgccgcgg acgccgugac caacgccacc     3780

gagcuggccu ucucccucga cgagaugaag aucaucagca cgugcgccaa gugcaucaac     3840

ggcgcggacc cguccgccau cgacaagauc gggaacgccc aggacagcuu cuucgacaac     3900

cugaagcgcc ggggcuccga ccgcugcgcc cuccuguuca gccagcugaa gcuggacaag     3960

cucucccaga acuucuacau gaucagcucc gacgacaaga gcaagauccu gaucagcauc     4020

cuggaccgga uccgcgccaa guccaacagc guggaccucu cccuggucgg cggggcgaag     4080

cacgccgccc agaugaaguu cgacuucaac gccaagcuga acaaccccgc gagcuccuuc     4140

gugcucaucg accagagcgu gaccggcaug uucgagcgcc ggacccgcau cgggcuguga     4200


<210>  2129
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2129
augaaccugc gcaacgccac cggcgacuac agcaucgggc ucgaccuggg caccaacucc       60

gugggguggg ccgucacgga cagcaacggc uccauggcgc acuucaaggg gaagcccacc      120

cugggcagcc ggcuguucga cgccgccgag accgccgccg gcgcgcgcca gcaccggggg      180

cagcgccggc gcuacgugcg gcgccggugg cgccuggacc ugcuccaggg ccuguuccag      240

gacgagguga acaaggucga cuccgaguuc uucauccggc ugcgccagag ccggcuccac      300

guggaggacc gcgccgaggg gcacgccggc uaccgguggc cgcuguucaa cgacaccgac      360

uucacggaga aggacuacua caagcgcuuc cccaccaucu accaccuccg gaaguggcuc      420

auggagaccg acgagaaggc cgacauccgc cugaucuacc uggcggccca caacaucgug      480

aagcaccggg ggaacuuccu ccgcgaggac aacaagaacc uguccagcca caacgccaac      540

cccgccgagg ccguccgguc ccugcgccag gcgcucgacg acuggugcgc cgagcgcggc      600

uacgugugcg ccuggaccag cguggacgac cuggccgagg ccgacuccca cauccuggac      660

auccucacga acagcaagga gcggcgcgcg gaccaggcca aggagcuggc caagcuguuc      720

ggcgucgacu gcggggacac caagcagucc aagagcugca acuccgcccu cgccaaggcg      780

guggugggcc ugaagaccga gcugaaggac gucuucgggg aguucugcgc cgagaagacc      840

gccauccacc ucagcuccga cgacgacgug gaggccaucc gggacgccau ccccgacgac      900

agcggcgagc uguuccaggu gcugugcgcg gucuacuccg ccuacgugcu ccaggagcug      960

cugagcuacg ccccgggcca gacgaucagc gccaacaugg uggcgaagua cgaccaguac     1020

gggaaggacc ucaagcugcu gaagaaccuc gcccgcaccu acgucucccg ggagaacuac     1080

gacgccuucu uccgcggcca gaccuacgag gacagcaacg acuacaacgu ggacaaggcc     1140

cagggguaca cccgguacaa ccugggcacg accaaggccu ccuacgacga cuucaagaag     1200

uccguggaga agcuguucaa ggggacccag gcggagcagg acaagcgcua ccagggcaug     1260

augaagcggu ucgccgagga gaaguuccuc cgccggcuga agaccuccga caacggcagc     1320

aucuacuacc agcugcaccu cgaggagcug cgcgccaucc ugaagaacca ggggaagcac     1380

caccccuucc uccuggagga ggagaagaag aucgaguccc uggucagcuu ccggaucccc     1440

uacuacgugg gcccccucuc cagcaagaac gccucccugg accacaaggg gaagcagcgc     1500

uucgccugga gcgagcggcg cccgggcaug aaggacacgg ugaucacccc cuggaacugg     1560

gaccagauca ucgacugcga ccaguccgcg gagaaguuca uccugcggau gaccggggac     1620

ugcaccuacc ugcagggcga gcccguccuc cccaagugca gccugcugua cgaggaguac     1680

ugcgugcuca acgagcugaa cggcgcccgc uggacgaucg acggggacga cuggcggcgc     1740

uucgacgucg cccagcgcga gggcauccug cacgaccucu uccggaagac ccgcuccgug     1800

gccuacaaca agguggccga cuggcuggag cgggaggaga acugggcgaa cgcccacguc     1860

cgcgggggcc agggggagaa cagcuucgag uccaagcugg gcagcuacau cuucuucugc     1920

aaggacgugu ucaagaccgc cgcccucgac gaggcggacu acccgaugau cgagcagauc     1980

auccugugga acacccuguu cgaggaccgg gccauccuca agcgcaagau cgagcaggcc     2040

uacggcgagc accacggggg caagcugacg gacgagcaga ucaaggugau cugcaagaag     2100

cgguucaccg gguggggccg ccugucccgg aagcuccuga ccggcaucaa ggucgacgcc     2160

gacaccgggc gcgugagcau cauggacgug cugcgggagg gcaaccccaa cgaggggcag     2220

cgcagccgga cgaugguccu cauggaggug cugcgcgacg aggaccuggg cuuccagaag     2280

aagaucgacg acuucaacaa ggccuacuuc gcggggcagg gcgagggccu cauguccgug     2340

aacgaccugc ccgggagccc cgccauccgg cgcuccauca accaggccau ccggaucguc     2400

gacgagaucg ccagcaucgc cggcaaggcg ccguccaacg uguucaucga ggugacccgc     2460

gaggaggacg acccccggaa gaaggggcgc cggaccaacc gccgcuacga ggacaucaag     2520

aacgcccugg agaacuucaa gaccgaggac caggagcucu ggagcgagcu gcugggcaag     2580

gcccccaacg accucgacga gcggcugucc cuguacuuca ugcagcgcgg gaagugcaug     2640

uacagcggcc ggcccaucaa gcucgaggag cugcacacgg gcaaguacga ggucgaccac     2700

aucaucccgc gcuccuacau caaggacgac agcuucgaga acaaggugcu ggugugccgg     2760

gaggagaacc agcgcaagac caacgagaug cucaucgacc ccgccauccg gcgccggaug     2820

ggggacuacu ggcgcaugcu gcacaaggcc aagcugaucg gcgacaagaa guucaacaac     2880

cucuucaugc gggaccgcau cgacgacaag acccugaagg gguucgucgc gcggcagcug     2940

guggagaccu cccagagcgu gaagcucguc cagacccugc uggaggugcg cuaccccaac     3000

acgaagaucg ugcccgucaa ggccaacauc ucccacgacc uccgggaggc cgccgaccug     3060

gugaagugcc gcgaggcgaa cgacuuccac cacgcccacg acgccuaccu ggccugccgg     3120

gugggccucu ucauccagaa gcgccacccg agcgucuacg ccaaccccau cgggcugucc     3180

cgggugaugc gcgacuacgc gcgcaagcag gccggcgagu ucaacaagac ccacagcaug     3240

gcccacacca agggcuucau cgugaacucc uucaugacca gcgaguucga caaggagacg     3300

ggggagaucu ucugggacgc cgaggccgcg gucgaggaca uccggcgcgu gcugaacuac     3360

cggcagugcu ucaucagccg caugcccgug gaggacaccg gcgccuucug ggaccagacc     3420

aucuacagcc cccggagcaa caagcgccuc gagcugccgc ugaaggacga ccucgacccc     3480

aacaaguacg gguccuacag ccgggagcag uucgccuacu ucuucaucua cgaggcccgc     3540

gacgccaaga aguccaagac cguccugcgg uucgcccagg ugcccgugcg cgucacgagc     3600

gcgaucgccc gggacuccag caagcuggac gaguacgccu ccggccucgc cgugcaggag     3660

gggcuggagu ucaccaagau cauccgccgg cgcauccuga agaagcagcu ccuggagcug     3720

aacggcgacc ggcucgugau caccggcgcg aaggaggucc gcaacgccac cgaguucgcc     3780

uucacgcugg aggagaccgc ccugguggcg augugcagcg agaucgcccg ggagggggug     3840

ccguccgaga ucgccagcga cguccagucc aucagcuccc agcucagcga ccuguuccug     3900

gcccucaucg agcgcggccu gaccaucggg gugcggcugc acggccagcu caagcugggc     3960

caguaccugc gccaguucga ggacgccgcg cuccgggaca aggccaaggu gaucaucggg     4020

aucaucucca ucaccaacgg cgccacgaac acccuggacc ugagcucccu caacgggagc     4080

aagaacgucg gccaggugcg caucacccac aacaagcugc ugaacgacaa gagcaccucc     4140

uacgugcuca ucgaccagag cgucacgggg auguucgagc gccggacccg caucggccug     4200

uga                                                                   4203


<210>  2130
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2130
augaagacca ucaccgagga cuacauccug ggccucgaca ucgggacgaa cagcugcggc       60

ugggccguga uggacaagaa gaaccgccug cugcggcucc gcgggaagcg ggccaucggc      120

ucccaccugu ucgaggaggg gcacagcgcg gccgaccgcc gguccuuccg caccacccgg      180

cgccggcuga agcgccggaa guggcgccuc cggcugcugg aggaguucuu cgccgagccc      240

auggccaagg ucgacccggc cuucuucgug cgccuccacc agagcugggu guccccgcug      300

gacaaggacc ggaagaagua ccacgcgauc gucuucccca ccgccaagga ggaccacgag      360

uucuaccacg acuaccccac gaucuaccac cuccgcgacg cccucaugcg ggaggaccgc      420

caguucgacc ugcgggagau cuuccuggcc auccaccaca ucgugaagua ccgcggcaac      480

uuccuccagg acaccccggu gaacagcuuc gucgcgucca agaucgacgu ggccagcuuc      540

cugaccaacg ugaaccaggc cuacgccgac aaguacggcg aggagcagaa cuucaucgag      600

cugaacgaga acaacgccgc ggccaucgag gccgucaucc gggggaagga caaggacaag      660

accacgaagc agcucgacaa ggccaagaag aucaccaagc ugcugcaguc cagcaccaac      720

aaguacgaga agaacgugac ccgccagauc gccaacgcga ucaucggcua caagacgcag      780

uucgagacca uccucgggaa ggacaucgac aagaacgaca aggccgacug ggaguucaag      840

cuguccgacg gcgacgccga cgacaagcug gagaaccucc ugcccgaguu cgacgacaac      900

gaccaguaca ucguggccga gaucaagaag cuguucggga gcaucgcccu cucccagauc      960

gucuacgaga acaugacccu gagccagucc augaagugca aguacaagga ccacggcgag     1020

gacuacgacc ugcucaagca guacaucgcg acccugagcg accacuccca ggugcgcaag     1080

cugcuccugg ccuacgaccu guacgugaac aaccggcacg gccgccuccu ggaggccaag     1140

aaggaguuca agcaggacaa ggcccugacg caggacgagu ucuacaagau cgucaaggag     1200

aaccucgacg acagccagcu ggcccagaag auccugcaga agaucgacga ggggaaguuc     1260

augcccaagc agcgggugaa caagaacggc uacauccccu accagcucca ccagaucgag     1320

cuggaccaga ucaucgcgaa ccaguccaag uacuacccgu uccuggccga ggagaacccc     1380

gugaaggagc accgcaagca ggcccccuac aagcucgacg agcugguccg guuccgcgug     1440

cccuacuacg uggggccgcu gaucagcccc gacgacaagc ggaaggacga gcagcagaac     1500

aagaaccacg agauguucaa guucgccugg augaagcgca aggacgacaa cggcgcgauc     1560

acccccugga acuucgccca gaaggucgac cgggagacca gcgccaaccg cuucaucaag     1620

cggaugaccg ugaaggacac guaccucauc ggcgaggacg ugcugcccgc ccgcucccug     1680

cuguaccaga aguucgaggu ccucaacgag cugaacaaca uccgggugaa cgggaagcgc     1740

cuggagccga aguucaagca gaaggucuuc aacgagcugu ucaagcagaa gaacaaggug     1800

accaagaagg ccuucaagga guucauccug cagaacaccg gcaugaagac cgcggagauc     1860

gacgggcugg ccgaccccac gaaguucaac agcucccuca gcuccuacua ccaccucaag     1920

agcaccaagg uguucgacaa gcagcuggac gacccccagu accaggccga cuucgagaag     1980

aucaucgagu acuccagcau cuucgaggac gccgagaucu accaggagaa gcuccacaag     2040

gagaucaagu ggcugacccc cgcccaguuc aaggcgaucg ccaacuggcg gcugcagggc     2100

ugggggcgcc ucucccggaa gcugcugguc gagcucaagg gcaagggcgg ggagaacauc     2160

aucuaccagc uguggaccac gcagaagaac uucaugcaga ucgugaacga gccggagcug     2220

aagaaggcca ucgugaagga gaaccaccag gccaccgagg agaagggcau cgagaagauc     2280

cucgacgaca gcuucaccuc ccccgccaac aagaaggcga uccgccaggu caucaaggug     2340

guggacgacg ucgugaaggc cgccgggggc aagaagcccg cccaguucgc gaucgaguuc     2400

acccgggagc ccaaguggaa cccgcaccug agcaacaucc gcgggacgaa gcugcuccag     2460

guguacgagg acaccgccaa gaagcugguc gaccaguccc ugaccgacag ccuccgcgag     2520

aacaugacca accggaaccu gcugaaggac gaguacuucc ucuacuucau ccagggcggc     2580

cgcgacgccu acacggggaa gaagaucgac aucgacgaca uccccaagaa guaccagauc     2640

gaccacaucc ugccccaguc cuucaucacc gacgacagcc uggacaaccg ggugcucacc     2700

gagaucgaga ucaaccagaa gaagggcaag aaguacgccu cccaccuguu cgcccccaag     2760

ccggcguucg ggcugaacca gagcaucucc gagaugugga agaccuggca gaaggccggc     2820

cucaucagcc agcgcaagcu gaacaaccug cuguucgacc ccgaccuggu gaaggacaag     2880

cagaagaagg gguucaucaa ccggcagcug gucgagacca gccagaucau caagcucgug     2940

uccaucaucc ugcaggacaa guuccccgac acggagauca ucaccgugaa ggccggcuac     3000

aacaccgccc ugcgcaagga gcucaagcug uacaagagcc gggaggccaa cgacuaccac     3060

cacgcgaucg acgccuaccu gaccgccauc ugcgacaacu uccucuacca ggucuacccg     3120

aacuuccgcc ccuucuucgu guacggccag uucaagaagu ucucccagga cacggacaag     3180

cagaagaagg ccgugaagua cuacgggcug aagaacuucc ugcucccccu gcuggacgac     3240

agcaccggcg acgaggucau ggugaaccac accaagcacg uggucuucca caagcagcgg     3300

gacaucuuca accgccucau ccaggccuac aacuacaagu acaugcuggu gucccgggag     3360

accacgaccg agaacagcaa cauguucaac augaccaucu acccccgcgg ggagcgggac     3420

cgcaagaaga cccggaagcu gaucccgaag ggcaaggacc ucgaccccgc gaucuacggc     3480

ggguacuccg gcaacacgga cgccuacaug gugaucguca agaucgagaa gaccaaggag     3540

acccuguaca aggugguggg gguccccaug cgcgcccugg ccagccucaa gaaggcgcag     3600

aaggagggca acuacgacca ggagcugaac aaggugcugg agccccucau cauguucaac     3660

aagaacggga aguccaaggc cggcaucaag ggcuucaccg uggucaagga ccacgugcac     3720

uucaagcagg ucgugaucga cggggacagc aaguucaugc uguacuccgc ccaguaccgg     3780

gccaacgcca agcagcugac gcucagccag gagaccauga agguggugac cgacaacacc     3840

gacgacaugg accagaagaa cgcggacaag gaccagcugc ugcucaacgc cuacgacgag     3900

auccuggaga aggucgacca guaccugccc cucuucgaca ucaacggcuu ccgccagggg     3960

cugcacgacg gccgggccaa guucgccgcc cugaagacgu ccgagaagaa guacacccuc     4020

aagaacgugc ugaacgggcu gcacgacaac cucgugaccc ccaaccugaa gaacaucggc     4080

aucaagaccc ccuucggccu gaugcagagc uccagcggga ucacgcucuc ccccaacgcg     4140

aagcuggucu uccagagccc gaccggccug uucgagaagc gcgugaagcu caccaaccug     4200

uga                                                                   4203


<210>  2131
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2131
augggcaagc agaaguucaa cgacuacuac cugggguucg acaucggcac caacagcgcc       60

gggugggccg ugaccgaccu ccaguacaag cugcugcgcu ucaacaagaa ggacaugugg      120

ggcucccgcc ucuucgacga ggcgcagacg agcaaggagc ggcgggucua ccgcaacucc      180

cggcgccggc uggaccgccg gaagaagcgc cugaagcucc uggaggagcu guuccucgac      240

gagaucagca agaucgacaa caccuucuuc aagcggcuga aggaguccaa ccuguacaac      300

gaggacaaga gcaucaaguu caacuacccc cucuucaacg acaaggacua caccgacauc      360

gacuucuaca acgaguacaa gaccaucuac caccuccgca aggaccugau cuucgaggac      420

aagaaguucg acauccggcu cguguucauc gcccugcacc acauccugaa guaccgcggg      480

cacuucaucu ucgagggcca guccuucgag gagaucaaga gcuucgacuc caucuucacg      540

cagcucagcu ccuacuuccu ggagaacuuc gacuucaaca ucgacgugag caucaaggag      600

aagcuggagg agaucauccu cuccgacaac accaagagca ucaagaagac cgaguucaag      660

aagaucuucu ccgacaacaa gguccugagc gugcugaaca uggccaucgg cuccaaggug      720

agccucaagg acgucuucaa caacgaggag cugaaggagc agaagaucuc cuucagcgag      780

aucgacuacg agaacgagaa gcgggacgag uucuaccagc ugcucgggga ggacaucuuc      840

cugaucgacc uggccaagaa gaucuacgac uucauggugc ucaagaagau ccugaagaac      900

accgacagcu ucuccgccag caagguggcg cuguacugcg agcacaagaa ggaccucgac      960

gaccugaagu acaucuucaa gaaguaccug acgaaggagg acuacuucaa guucuuccgc     1020

uccaacgucg agaagaucaa cuacccgagc uacaucggcc ucaacaagau caaguccaag     1080

aagaucauca aggagaacaa gugcagcauc uccgacauca acaaggagcu gaagaacaag     1140

uaccugaagg acaucaagcu cgaggccaag gacaaggagc uguucaaccg gaucuucaac     1200

aagcuggacg aguucaaguu ccucgagaag cucaagagcu ccgagaacag caccgugccc     1260

uaccaggugc acgagaucga gcugaagaag auccucgaca accagcgcaa guacuacccc     1320

uuccugaacg agaagaucga cgggaagucc gucuacgaga agaucaucca gcuguucgag     1380

uucaagaucc ccuacuacau cggcccgcuc aacaccgacc ccaacgugaa ggggaagaac     1440

accugggugg uccggaagga cgagaacaac aagacgccca ucacccccug gaacuucgag     1500

cagaaggugg acaugcagaa guccgccgag gaguucauca agcgcaugac caaccggugc     1560

accuaccuga aggccgagga cgugaucgcg aagaacagcc ugcuguacga ggaguucauc     1620

cuccugaacg agcugaacaa ggucacggug gacaacgaga uccuccaccc gacccugaag     1680

accgagauca ucaacgagcu guucaagacc acggugaagg ucaccaagaa gaaguucauc     1740

gaguuccuca agaagaagca ggccgugaac gacguggaga gcaucaccgg caucggcguc     1800

gaguucaacu gcaagcugca gggguacaac accuuccgca agauccuggg caagaagauc     1860

gagaacguga acgucaagaa cuucguggag aagugcaucg agcucaagug ccuguacggg     1920

uccgacaaga agcuguucga gaacgccauc ggcaagaucu accgcgagua caacaucacg     1980

gacgaggagu ucaacaagau caaccggcuc aacuucaaca agugggcccg ccugagcaag     2040

aaguuccuga ccgagaucuc cuucgagaag accgacggca ccaagcgguu cgagagcgug     2100

aucgaggccc uccgcuccac gaacuacaac cugauggagc ugcucagcga caaguucaac     2160

cugcagagca agaucgacga gaucaacggg gaggacgacu ucuccaacau cgacaucaag     2220

gaguacgucg acaagcugua cgugagcccc uccgugaagc ggagcaucau ccagaccuac     2280

aagaucgcga uggagaucaa gaagaucacc aagaaggacc ccaagaagau cuucaucgag     2340

auggccaagg acaagggcga gaaggggaag accacggaga agcgcaagga caagaucaag     2400

aagcucuaca agaacaucaa ggacacccug uccgacuacu gcaacaaccu gcaggaccuc     2460

acccugcagg ucgagaagua cgacgacaac cggcugcgcc agaagaagcu guuccuguac     2520

uucaugcaga ugggcaagag cauguacacc gggcggugca ucgaccugga ggaccucuuc     2580

aacaacaacg uguacgacau cgaccacauc uacccccagu ccaaggugaa ggacgacagc     2640

uucgacaaca ucguccuggu ggagaaggag gagaaccacc gcaaguccaa cgagcugcuc     2700

aaggaggaga uccagagccg gaugaacaac uacuggaucu uccugcgcga gaacaaguuc     2760

aucuccaagg agaaguacaa ccggcugacg cgcaagaagg acuucaccga cgaggagcuc     2820

ggcgacuucg uggcccggca gcuggucacc acccgccaga gcaccaagga gaucgccaag     2880

uucuucgaga agaucuucga gaagacgacc gugguguacu ccaaggccag ccuggucucc     2940

gaguuccggg agaaguacga caucaucaag gugcgcgagg ugaacgacgc gcaccacgcc     3000

caggacgccu accucaacau cgucgugggc aacguguuca acaccaaguu caccaagaac     3060

uacuggaagu ucacgaagga caucaagcgg cagaacgagg agaacccgaa cgagaagaag     3120

ggguacaacc ugcgcaagau cuacgagagc gacgucuucu gcaacgacuc cguggccugg     3180

guguacagca aggagaaccc caaguacaac accaucaaca ccgucaagga caugguguac     3240

cgcaagaccg ccaacaucac gcggaugcug guggagggca aggggcagcu guucaaccug     3300

accaucaagg acaagaccca gcugaagaag aacgucggca acaucguggc gaacaccccc     3360

aucagccuca agaaccgcaa gcugcaccug aacuccgugg agaucgccaa gaaguacggg     3420

uacuucgaca gccucaacaa cgccuacuuc guccuguuca aguacgagga gaagggcaag     3480

gagaagaucg gcuucgacuc caucacgauc gccuacaagg agcggcugaa gaagaaccuc     3540

gacaucgaga aguuccugga gaacaagggg uacaagaaca gcaagcugau ccgcaagauc     3600

uucaagaacc agaaggugcu caucaacggc uaccccuacc ugcugaaguc cuucaacaag     3660

cagagccaga ucaaguucaa caacuucaag auccucuacc ugaacgagaa caaccugaag     3720

uucgccaagg acgcgaucaa guucgagaag aacaacgcca acguggaccu caagaagcag     3780

aaguacaucg ucuccuuccu gaagaagaag aagcagaacg agaucaacga gaccuacgag     3840

gagugcaucg accggcugaa cgacgaguuc gacacccucu uccugaagcu gugcgacauc     3900

cagaagucca gcaucuacac caacuacaag cuccaggacu ucagcuccaa caagaagucc     3960

aucgagauca aggacaaguu cgacaucuuc aagagccugg accuguacaa gaaguccaag     4020

cucaucaagg aguuccugaa cuacuacaag uacgagaaga acgaccugga cuucagccuc     4080

aucgacaucg acaagaaggc caacugcauc cagaagcacu ucacggagga ccuggugaag     4140

gacuucguga ucgucgacga gagcgugacc gggcuguucg ugaagaagga gcgccucaag     4200

uga                                                                   4203


<210>  2132
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2132
augaagacca ucaccgagga cuacauccug ggccucgaca ucgggacgaa cagcugcggc       60

ugggccguga uggacaagaa gaaccgccug cugcggcucc gcgggaagcg ggccaucggc      120

ucccaccugu ucgaggaggg gcacagcgcg gccgaccgcc gguccuuccg caccacccgg      180

cgccggcuga agcgccggaa guggcgccuc cggcugcugg aggagaucuu cgccgagccc      240

auggccaagg ucgacccggc cuucuucgug cgccuccacc agagcugggu gagcccccug      300

gacaaggacc ggaagaagua ccacgcgauc gucuucccca ccgccaagga ggaccacgag      360

uucuaccacg acuaccccac gaucuaccac cuccgcgacg cccucaugcg ggaggaccgc      420

caguucgacc ugcgggagau cuuccuggcc auccaccaca ucgugaagua ccgcggcaac      480

uuccuccagg acaccccggu gaacagcuuc gucgcgucca agaucgacgu ggccagcuuc      540

cugaccaacg ugaaccaggc cuacgccgac aaguacggcg aggagcagaa cuucaucgag      600

cugaacgaga acaacgccgc ggccaucgag gccgucaucc gggggaagga caaggacaag      660

accacgaagc agcucgacaa ggccaagaag aucaccaagc ugcugcaguc cagcaccaac      720

aaguacgaga agaacgugac ccgccagauc gccaacgcga ucaucggcua caagacgcag      780

uucgagacca uccucgggaa ggacaucgac aagaacgaca aggccgacug ggaguucaag      840

cuguccgacg gcgacgccga cgacaagcug gagaaccucc ugcccgaguu cgacgacaac      900

gaccaguaca ucguggccga gaucaagaag cuguucggga gcaucgcccu cucccagauc      960

gucuacgaga acaugacccu gagccagucc augaagugca aguacaagga ccacggcgac     1020

gacuaccacc uccucaagca guacaucgcg acccugagcg accacggcca ggugcgcaag     1080

cugcuccugg ccuacgaccu guacgugaac aaccggcacg ggcgccuccu ggaggccaag     1140

aaggaguuca agcaggacaa ggcccugacg caggacgagu ucuacaagau cguccgggag     1200

aaccucgaca acucccagcu ggcccagaag auccugcaga agaucgagga ggagaaguuc     1260

augcccaagc agcgcgugaa caagaacggc uucauccccu accagcucca ccagaucgag     1320

cuggaccaga ucaucgcgaa ccagagcaag uacuacccgu uccuggccga ggagaacccc     1380

gugaaggagc accggaagca ggcccccuac aagcucgacg agcugguccg cuuccgggug     1440

cccuacuacg uggggccgcu gaucuccccc gacgacaagc gcaaggacaa gcagcagaac     1500

aagaaccacg agauguucaa guucgccugg augaagcgga aggacaacaa cggcgcgauc     1560

acccccugga acuucgccca gaaggucgac cgcgagacca gcgccaaccg guucaucaag     1620

cgcaugaccg ugaaggacac guaccucauc ggcgaggacg ugcugcccgc ccggagccug     1680

cuguaccaga aguucgaggu ccucaacgag cugaacaaca uccgcgugaa cgggaagcgg     1740

cuggagccga aguucaagca gaaggucuuc aacgagcugu ucaagcagaa gaacaaggug     1800

accaagaagg ccuucaagga guucauccug cagaacaccg gcaugaagac cgcggagauc     1860

gacgggcugg ccgaccccac gaaguucaac uccagccucu ccagcuacua ccaccugaag     1920

uccaccaagg uguucgacaa gcagcuggac gaccccgagu accaggccga cuucgagaag     1980

aucaucgagu acagcuccau cuucgaggac gccgagaucu accaggagaa gcuccacaag     2040

gagaucaagu ggcugauccc cgcccaguuc aaggcgaucg ccaacuggcg ccugcagggc     2100

ugggggcggc ucagccgcaa gcugcugguc gagcucaagg gcaagggcgg ggagaacauc     2160

aucuaccagc uguggaccac ccagaagaac uucaugcaga ucgugaacga gccggagcug     2220

aagaaggcca ucgugaagga gaaccaccag gccacggagg agaagggcau cgagaagauc     2280

cucgacgacu ccuucaccag ccccgccaac aagaaggcga uccggcaggu caucaaggug     2340

guggacgacg ucgugaaggc cgccgggggc aagaagcccg cccaguucgc gaucgaguuc     2400

acccgcgagc ccaaguggaa cccgcagcug ucccacaucc gcgggaccaa gcugcuccag     2460

guguacgagg acacggccaa gaagcugguc gaccagagcc ugaccgacuc ccucaaggag     2520

aacaugacca accggaaccu gcugaaggac gaguacuucc ucuacuucau ccagugcggc     2580

cgcgacgccu acaccggcaa gaagaucgac aucgccgaca uccacaagaa guaccagauc     2640

gaccacaucc ugccccagag cuucaucacg gacgacuccc uggacaaccg ggugcucacc     2700

gagaucgaga ucaaccagaa gaagcacaag aaguacgcca gccaccuguu cgcgaccaag     2760

cccgccuucg ggcugaacca guccaucagc gagaugugga agaccuggca gaaggccggc     2820

cucaucuccc agcgcaagcu gaacaaccug cuguucgacc ccgaccuggu gaaggacaag     2880

cagaagaagg gguucaucaa ccggcagcug gucgagacca gccagaucau caagcucgug     2940

agcaucaucc ugcaggacaa guucccggac acggagauca ucaccgugaa ggccggcuac     3000

aacaccgccc ugcgcaagga gcucaagcug uacaaguccc gggaggcgaa cgacuaccac     3060

cacgccaucg acgccuaccu gaccgccauc aacgacaacu uccucuacca ggucuacccc     3120

aacuuccgcc ccuucuucgu guacgggcag uucaagaagu ucagccagga cacggacaag     3180

cagaaggagg ccaucaagua cuacggccug aagaacuucc uggagccgcu ccuggacgac     3240

uccaccggcg acgaggugau ggucaaccac accaagcacg ugguguucca caagcagcgg     3300

gacaucuuca accgccugau ccaggcguac aacuacaagu acaugcucgu cagccgggag     3360

accacgaccg agaacuccaa cauguucaac augaccaucu acccccgcgg ggagcgggac     3420

cgcaagaaga cccggaagcu gauccccaag ggcaaggacc uggaccccgc caucuacggg     3480

ggcuacagcg gcaacacgga cgccuacaug gugaucguga agaucgagaa gaccaaggag     3540

acccucuaca aggucguggg ggugccgaug cgcgcccugg cgucccugaa gaaggcccag     3600

aaggagggca acuacgacca ggagcucaac aagguccugg agccccugau cauguucaac     3660

aagaacggga aguccaaggc cggcaucaag ggguucaccg uggugaagga ccacguccac     3720

uucaagcagg ucgugaucga cggcgacucc aaguucaugc ucuacagcgc ccaguaccgg     3780

gccaacgcga agcagcugac gcugucccag gagaccauga agguggugac cgacaacacc     3840

gacgacaugg accagaagaa cgccgacaag gaccagcucc ugcugaacgc cuacgacgag     3900

auccucgaga agguggacca guaccugccc cuguucgaca ucaacggcuu ccgccagggg     3960

cuccacgacg gccgggccaa guucgccgcg cugaagacga gcgagaagaa guacacccug     4020

aagaacaucc ucaacgggcu gcacgacaac cuggucaccc ccaaccucaa gaacaucggc     4080

aucaagaccc cguucgggcu gaugcagucc agcuccggca ucacgcugag ccccaacgcc     4140

aagcucgugu uccagucccc caccggccug uucgagaagc gcgugaagcu gaccaaccuc     4200

uga                                                                   4203


<210>  2133
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2133
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaag       60

gagacggaga acuccaacga gaaguccgag aucaucaagc ucgggguccg cgugaacccc      120

cugaccgugg acgagaagac caacuucgag gccggccggc cgcugagcac caacgcggac      180

cgcacggcca agcggagcgc ccgccggaac cuccagcgcu acaagcugcg gcgcaagaac      240

cugaucgacc uccugaucaa gcaccggcug aucgacaagg acacgccccu caccgagauc      300

gggaagaaca ccacccacca gacccuggag cugcgcgcca aggccgcgcg ggagcgcauc      360

gagcucgagg accuggcccg ggucuuccug gccaucaaca agaagcgcgg cuaccggucc      420

agccgcaagg ugaacaacga ggaggagggc cagguggucg acgggauggc cguggcgaag      480

aagcucuacg acgagaaccu gacccccggc caguacgccu acgagcugcu cuccaagggg      540

aagaaguacg ugcccgacuu cuaccggagc gaccugcagg ccgaguucga cuccaucugg      600

gaguaccaga agcaguucua cgccgacauc cuggacgacg agcucuacaa cgcccugaag      660

ggccaggggc agcagaacag ccgcaagcgg uuccuggcga ucaagggcgu cuacaccgcc      720

gagaacaagg gcaagcgcga cgaggugaag cuccagcacu acaaguggcg guccgaggcc      780

aucacgcaga agcugagcau cgaggaggug gccuacgucc ugguggagau caacaacgac      840

cucaacaagu ccagcgggua ccugggcgcc aucuccgacc gcagcaagga gcuguacuuc      900

aacaaggaga ccguggggga gaaccucugg aagcagaucc agaagaaccc gcacaccucc      960

cugaagaacc aggucuucua ccgccaggac uaccuggacg aguucgagca gaucugggag     1020

acccaggcgc aguuccaccc ccagcucacg cuggcccuga aggagcagau ccgggacgug     1080

gugaucuucu accagcgcaa gcucaagagc cagaagggcc ugcuguccuu cugccaguuc     1140

gagagcuggg agaucgagcg gaaggacgag aacggcaacg ucauccucaa caagaccacc     1200

cagcugccca agcgccagac cguggggcgg cgcguggccc ccaagagcuc cccgcuguuc     1260

caggaguuca agaucuggca gaacaucaac aaccucgaga ucgccaagau cagcgacggc     1320

aacuccaaga acaagaagga gacggaggcc cugagcgacg acgagcggaa gcuccuguuc     1380

gaggagcuga accugcgcgg gaaccugucc cagaaggagg uccuccagau ccugggccug     1440

aaggacaagg aguacaagac caacuucccc gaggggcucg agggcaaccg gaccaacgcg     1500

gcccuguuca acaucuacca gcagaucgcc gagaacgagg gcuacgggga cuggaccaag     1560

aaguccgccc aggagaucaa ggaggagcug aaggccgugu ucccccagau cggcauccag     1620

gcggacaucc ucgacuucaa cgccgagcug gacgggaagg aguucgagaa ccaggccagc     1680

uaccagcugu ggcaccuccu guacagcgcc gaggaggacg acaagaucaa cgaggaggac     1740

cagaucaucu acggcaacuc cgcggugagc cugaagaaga agcucugcga gaaguucggg     1800

uucacgcccg aguacgccaa guggaucgcc aacgucuccc ugcaggacga cuacggcaac     1860

cugagcacca aggccaugcg caagaucauc ccguaccuca ucgacggcaa cgacuacucc     1920

gaggccugcg cgcuggccgg guacaaccac agcaacuccc ugaccaagga ggagaacgac     1980

aaccgggagc gccucaacaa gcuggagcug cuccccaaga acagccugcg gaaccccgug     2040

gucgagaaga uccugaacca gauggugaac guggucaacc aggugaucga gaccuacggc     2100

aagcccgacg aggugcgcau cgagcucgcc cgggagcuga agaaguccgc cgaggagcgc     2160

gccgagauga cgaaggggau caacgaggcg acccggcgca acgaggacau caagaagcug     2220

aucaccaaga acuucggcau cccgaacccc accaagaacg acgucgugcg guaccgccuc     2280

ugggaggagc uggcgccccu ggccuacaag gacguguuca cggggcgcca gaucaagaag     2340

gaggaccucu ucagcuccaa gaucgacauc gagcacauca uccccaaggc ccugcuguuc     2400

gacgacagcu ucagcaacaa gacccucgcc uuccgggaga ccaaccugaa gaaggccgac     2460

cgcaccgccu acggcuucau cgaguccgac uacaacgcca cgcuggacga cuacauccag     2520

cgggucgaga cccucuacaa caacgccaag ggcaccauca gcaaggggaa gcgcaacaag     2580

cugcugaugg cgcagaagaa ccucccggac ggcuucaucg agcgggaccu gcgcaacucc     2640

caguacaucg ccaagaaggc caagagcaug aucgagaagg uguucaacga cgugaacguc     2700

accuccggga gcaucaccga ccggcugcgc gaggacuggg accucaucaa cgugaugaag     2760

gagcugaacu uccccaagua caaggcccug ggccucacgg agaucgagga gcgguacgac     2820

aucggccagg agaagaccaa gaagguggag gucaucaccg acuggaccaa gcgcaacgac     2880

caccggcacc acgccaugga cgcgcugacg guggccuuca ccaccaaguc ccacgugcag     2940

uaccugaaca accucaacac cauccacagc cugaaggggg acgaggucga ccuggagcuc     3000

uccaagcugu acggccugaa gaacacgauc accgaggugg ugaacaagaa gcgcaaguuc     3060

aucccgccca ugcccaacuu ccgggaggag gccaagaagc acaucgagac cauccucguc     3120

agcaucaaga acaagaacaa gguguacacc aagaacauca acaagacgaa gaaggagucc     3180

ggguaccaca ccaaggagca gcugacgccc cgcggccagc ugcacaagga gaccguguac     3240

ggcaagagca agcggcccau gaacaagccc accaagauca acaagaacuu cucccucgag     3300

caggcccagc ugaucaucaa cguccaggag aaggagcugg ugcuccgcca ccuggcccag     3360

uucgacaaca acccggcggu ggccuucgac accaagaccc ugaagaagau gccccuccug     3420

aagaacgggg agccccugaa ggagguccuc ugcuucgagg agaucuucac cauccggaag     3480

gacaucacgc cggaccugaa gaucgagaag gugguggacg agaagaucaa ggccauccug     3540

gagcagcgca agaaggacuu cggcgggaac gccaaggagg ccuucagcga ccucgacaag     3600

aaccccaucu ggcugaacaa ggagaagggc aucugcauca agcgggucac caucaccggg     3660

guguccaacg cggaggcccu gcaccaccag aaggaccacc ucggccgcga gauccuggac     3720

gacaacggga accccauccc cgccgacuuc gugagcaccg gcaacaacca ccacgucgcc     3780

aucuacgagg acgagaaggg caagcugcag gagaaggugg ugucccucua cgaggcgguc     3840

gcccgggcca accaggggcu gccgguggug gacaagggcc ugaacgagga ccucgggugg     3900

aaguuccugu ucacgaugaa gcagaacgag auguucgucu uccccaacga cgacuucgac     3960

gugaacgaca ucgaccugcu cgacgagaac aacgccaaga ucaucagcca gcaccuguuc     4020

cgcgugcaga agcuggccac caagaacuac auguuccgcc accaccucga gaccaacguc     4080

caggagguga aggagcugaa cgacaucgcg uacaagagca uccgguccac cgagccccug     4140

aagaacauca agaaggugcg caucaaccac aucggcaaga ucguccaggu gggggaguac     4200

uga                                                                   4203


<210>  2134
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2134
auggccaaga aggacuacac caucggccug gacaucggga ccaacagcgu gggcugggcc       60

aucaucgacg acaaccucaa gcugcugaag cgcaacauga cgaucaaggg gaacaccgac      120

aagaaguccg ucaagcggga ccucuggggc agccugcugu acuccgggaa cagcgacaag      180

accaccuccg cggccgacgc ccgcagcaag cggggccucc gccggcgccu gcggcgccgg      240

aaguaccgcc uggaccggcu caagcagauc uucuccgaga ucaucaacga caaggccccc      300

aacuucuucg acaagcugaa cgagagcuuc cugaacccga aggacaagaa guacggcaag      360

uaccagaucu ucgacacgga gaaggaggag aaggacuacu accgccggua ccccaccauc      420

uaccaccucc gcaaggaccu gaucgagucc agcaagaagc aggacauccg gcugguguac      480

cucgcccugg cgcacauccu gaagucccgc gggaacuucc ucuucgaggg caacaucgac      540

gaccugaaga acgacuucgc cgggaucuac gaggaggugg ucgagcugug caugaccauc      600

aacgccgagg acguggaccu cgaguucgag gagguggaca agcagagccu gaacuccauc      660

aucaagaacg aggacaucag cgagaucgag cagggccugg agaacuucgc cgacgagcac      720

gucaucuuca aggagcagaa caagaagaag aacgaccucu ucuccaacug cugcaagauc      780

aucugcgggc acaccgugaa ggcgaacaag uucgccagcg agcuggacuc cgagcuguuc      840

aucagcuuca aguccgacga cuacguggac gucaucgacg ugauccagag cggcaacgag      900

aacaucgcca accuccugcu ggccugccgg aaggccuacg acuacaucau guucaaccgc      960

cucguggacc ugaacaucga cagccccgcg aagcugucca gcaacauggu cucccucuac     1020

aaccagcacg agaaggaccu gaaggccuac aagaagcuga ucaaggaguu caacaaguuc     1080

aagcggagca acggcugcaa ggaccucgag augaucaucc ugacggccga cgacaucgac     1140

uccuuccgca agaaggugga caagaaggag gggaagcuga acggcaucaa caagaagauc     1200

acccacgagc aggcccucaa gaagcagcug aaggacauga agaagauccu ggaggacaag     1260

aacaccgagg ccgaggacaa gcagaucaac gacauccuca agaugaucac cagcaucgag     1320

gagcggguga acaaguccug cuuccugaag aaccugcgca gcacggacaa cgcguccauc     1380

cccaaccaga uccagcgcca ggagauggag gccauccucg acaagcaggc caaguucuac     1440

ccguuccuga acgagcacaa ggacgagcug cuccagcugc ugagcuuccg gauccccuac     1500

uacgucgggc cccuggugaa caagaaguac ucccgcuucg ccuggcucgu gcggaaggag     1560

ggccaggucc agaagaucac ccccaccaac uucgacggcg ugguggacaa gcacaagacc     1620

gccgagaagu ucauggagcg ccugaucggg aaggacgucu accugccgaa cgagcgggug     1680

cuccccaagg cgagccugcu guaccaggag uacugcaucu ucaacgagcu cacgaaggug     1740

gccuacaucg acuccaccgg caagaagaac aacuucagcu ccgaggagaa gcugaacauc     1800

uucgagaagc uguucaagac caagcgcgag gucaccaaga cggaccucug caagugccug     1860

aacaacgugu gcaagcugaa ggagaagguc aaggagaccg agaucaucgg gaucaaggcc     1920

aaguucaacg ccaaguacag caccuaccac gaccucaaga agaucaacgg cauggagcag     1980

cugaucgccg acgaggaggg gaagccccug ugcgaggaca ucaucuccau ccucaccauc     2040

uucgaggaca aggacauccg gcuggugcgc cugaaggagc uccugugcca gaacaaggac     2100

cugaucaaca aguucagccu cuccgcggag aagcuggcca aggugcugag cacgaagcac     2160

uacaagggcu ucggcaacgu cagcgccaag cucaucaacg ggauccggga caagaacugc     2220

aagaccaucc uggacuaccu gaucgaggac gacaaggagg ccuacuacgg ccgcaacaac     2280

cccaaccgga accucaugca gcuggugaac gacucccgcc uggcguucaa ggggcagauc     2340

gaccgggagc agaacaccca ccucgaggac cugagccugg acgaguuccu cgacgaccug     2400

uacguguccc cgagcauccg ccggggcauc cgccugacca uccggcucgu cgacgagcug     2460

guggagauca ugggguaccu gcccaagaac aucgugaucg agaugccccg cgaggacggc     2520

gagaagggca agaucgccga cacgcgguac uccaagcucg agaagaugcu gaagaaggac     2580

gccgcccugg aggaccucua ccgcguccug aagaccuacg agaagaacaa gaaggcccug     2640

gcgaacgacg cccucuaccu guacuuccug cagaacgggc gcgacaugua caccggcaag     2700

gagaucaacc ucagcgagcu gcacuccuac gacaucgacc acaucauccc caagagcuuc     2760

aaguacgacg acucccugga caacaaggug cucaccgcca agaagaugaa cauggacaag     2820

cggacggggg cccuggacca caacaucauc gagaaccagu gcggcuucug gcgcgugcug     2880

cuccagcagg acaagaucag ccuggagaag uacaccaacc ugaugaagac cgaguucacc     2940

gaggccgaca aggcgggguu caucaugcgg cagcucgucg agacgcgcca gaucaccaag     3000

uucguggccc gguaccugga caacaaguuc aacggccuga ucuccgaccc gaacgacaag     3060

gugaacaucc uccugccccg cgccagccug ugccaccagu uccgggagac cuucggcuuc     3120

uacaaggucc gcgagcucaa cgacaugcac cacgcccacg acgccuaccu gaacgcggug     3180

aucgccaaca cccugaacaa gaacgccuac cucuccgacc ugcugaagua cggggccuac     3240

agcaaguaca agaagaacgg cuucaacaac uccaacggga ucauggacua cuucggcaac     3300

acccaguuca acugccucuu cguggucgag cggacgcugg acaagugccg cgugaacauc     3360

gugaagcacc ccgagaccgc gagcggcgag uucuacaacg agaccaucca gaagaacaag     3420

gucaacgggg gcuccagcac ccggagccug aaguccagcg ugaaggugcu ccagaacacg     3480

gagcaguacg ggggcuucac caacgucaac aacgccuacu ucauccuguu cgacuacaag     3540

gccaagucca agcugaagcg caagcucauc ggggugccca ucguggaccg gcagaaguuc     3600

gagcaggacc cggucaccua ccuggaggcc aagggcuucg acgagcccaa gcuggugcag     3660

aagcuccuga aguacacccu gcucgaguac gaggacggca agcgccggua ccugacgggg     3720

gugaccggca agcgcugcga gcugguccgg gccaaccagc uccugcugcc ccgcaacaug     3780

auggcgcucc ugcaccaccu ccaggagugg cagaagcacg acuucgggau caaggagaug     3840

accaagguga ucaagaacac caacaacauc gaggccaagu ucgacaagcu guucgagcac     3900

augaugaagu ucaucgacaa guacagcgag cccccgaaga ucguguccag caagaucucc     3960

gaggaguacc acaagcugcg ggagagccug ugccaggacg acaacaagau caagaucuac     4020

gccgagaucg gcaaggcccu ccugucccug cugcaccucg ucgacagcaa guccgccugc     4080

guguucaagu ucagcgggcu ggagaucaac cgcauccggu accaguccau caacgagaag     4140

aaggagcccg ugaucaucuu ccagagccug uccggccucc gcgagagccg cuacaaguac     4200

aaccaguga                                                             4209


<210>  2135
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2135
augaagcugc gcaacgccaa ggacuacagc aucggccucg acguggggac cggcuccacc       60

gggugggccg ucacgacccu ggacggcgac cuguucaccu ucaaccaccg gcccacccuc      120

gggagccgcc uguucgaguc cgcgagcacg gcccaggaga cccgggaggc ccgcggcgcc      180

cggcgccggc ugaagcgccg gcgcuggcgg aucucccucc ugcaggagca guucgccgcg      240

gagauggaga gcugcgacuc caacuucuuc auccgccuga accagagccg gcucuggcag      300

gaggaccgca ccgagcaggg caagggguac cuguucggcg ggaagccgau ccugugcggc      360

gcccagaucu ucgccaacga gcaggaguac uaccagcagu uccccaccau cuuccaccuc      420

cggaaguucc ugguggagug cuccgagcag gucgaccugc gccucaucua ccuggcccug      480

cacaacaucg ugaaguaccg ggggaacuuc cucuaccagg acaacaagac gcugaacgug      540

cgcaacuuca accuggacag ccagaucgcg gacuuccucg accagcugca gcuguggugc      600

gagaacaacg accucugcuu ccacgagauc gacggcacca ccacccuggc cucccagcug      660

aacacgugcu uccaccagca gagcaaccag cagaagaagg acacccucaa gaccuucuuc      720

gcgaccugca ucgagaacga caagaucgcc ggcgagcuga cgaaggcccu gcucggguac      780

aagaccgacu ucaagaucau cuucaacauc gagggcgaga aggacgagua caaggccaag      840

ugcaacgagg aggagaagau ccugaagcug caggagacca ucgacgacgc caaccaggug      900

cuccugcagu ccauccugaa ccucuacagc gggaucaucg ucgagaagau ccugaacaac      960

accgccguga agaacgaguc cgcccagugc acgcugagcg ccuaccacug cuacuccuac     1020

gcgcucuaca gcaagcagcu gcggacccug aagaagcucg ugcgcaagua caacccccag     1080

cgguacaacg agugcuucaa ccuguccgcc gaggacccca aggccaagaa gcagcugugc     1140

accuacgccc gcuacaacgc ccagcacagc accgacuacg acgaguucaa gaaguacauc     1200

aaggaccucu ucgagaacac ggacgcgguc aacgacgccg ccuaccagca gaugaagcag     1260

gacuucgacg aggagaaguu ccugcgccgg cagaagacca gcgacaacgg cguguacccg     1320

uaccaguucc accuggagga gaugcagcuc aucaucgaga accaggccca gcacuacccc     1380

uuccugacca agaucaagcc caagcuggag ucccugguga ccuuccgcau ccccuacuac     1440

gucggcccgc ucacgcagaa ggggauccag cggaacggcc gcaaguucgc cuggagcaag     1500

cggaacaagg acgcggagaa ccagcgcauc uaccccugga acugggacuc caucaucgac     1560

aagagcgccu ccgccgaggc cuucauccug cggaugaccg ggaagugcac cuaccuguac     1620

ggcgaggacg ugcuccccaa ggagagccug cuguaccagg aguucugcgu gcucaacgag     1680

cugaacgggg cgcgcuggaa cugcgacggc gagcggcgca ugccccuguc caacaaggac     1740

ugcaaggcca ucuaccggca gcuguuccug aagaagcaga aggucagcua cgccgacgug     1800

uccaacuacc ugaaggccca gggccacauc aacaacagcg uggccggggg ccagggggag     1860

cacggcuucg aguccacccu cagcucccac cacuucuucc gcaagcuguu caacgucgac     1920

gcggccaacg accugacggc ccagcagauc gagaccgccg agcagcucau ccuguggggg     1980

accaucuucg aggacaagga gaucuucgag cagaagauca aggagcgguu cggcgacaug     2040

uucagcaagg acgagaucaa gaccaucgcc cgccggaagu ucuccggcug ggggaagugc     2100

agcaagcagc ugcucuccgg ccugacggug gagaccccgc gcgggcggga gagcaucauc     2160

gacgugcugc gcaacggcca ccccgagaac gggcggaccc ucgucuucau gcaggucauc     2220

accgacaaga agcuggaguu cgacaagaag aucgaggagu acaacaacga gcaccuggcg     2280

aagaccaacg aguccgugcu cgacacggcc aucaugagcc ccgccaucaa gcgcggcgug     2340

aaccaggccc ugcacaucgu gggcgagauc cgccggauca ccaagcacga cccgaccugc     2400

aucuacaucg agaccacgcg cgacgagaag gacgggaagc acaacacccg gaccagcucc     2460

cgcuacagcu cccugaagaa gaaccucgag acccucaaga gcaucgacaa ggccaacgcg     2520

cccgccauca agcacucccu ggagcuccug gagacguaca agaagcagaa caucgacgac     2580

aagcuguucc ucuacuucac ccagaacggc aagugcaugu acagcgggaa gucccuggac     2640

aucaacaacc ugagcuccua ccagaucgac cacgucaucc cccagagcua caucaccgac     2700

aacuccuucg acaaccgggu gcucguguac gccagcgaga accagcgcaa guccgacagc     2760

cugcugcucu ccagcgucau ccaggccaag caggagcccu ucuggaccuc ccuggagaag     2820

gcgggccuga ucagcgagaa gaagcacaac aaccucaagc gcaagagccu guccgacgcc     2880

cagaucaagg gcuucaucaa ccggcagcug guggagacga gccagaucgu gaagguccuc     2940

cgggacgagc ugucccgcau ccugcccgac accaagauca ucccggugaa ggccagccuc     3000

ucccacgacg ugcggaucgc cugcaaccug uacaagagcc gcaccgccaa cuccuuccac     3060

cacgcgcacg acgcccugcu cgccagcacc auggggcggu ucguccagac gcgcuucccc     3120

gacauguacg agaaccccgu gcgguacgug aagauccugc gcgccuacau cgcccagucc     3180

aagcugcagc acagcgcgca gacccggaac uucgacuccc gcagcuucau ggcccacucc     3240

uucuucugcg acuacagcaa cgagaacggc gacaccaucu ggaacuccgc cagcgagauc     3300

gagcggcucc gccgggcccu guccuacaag aaccuguacc ucacccgccg ccccuucauc     3360

gacgccgggc aguucuggga ccagacgauc uacagcccgc acgacaagaa gaagaccccc     3420

gacucccuga ucccccugaa gaagaaccuc gacacccaga aguacggcgg guacagcggc     3480

aaggacuacg cgcacuucuu caucuacagc guccugaaca agcagggcga cguggaguac     3540

cgguucgacg gggugccccu guacaucgcc gccaacauca accgcgacga ccggggcgcc     3600

cucgcgcagu acgccaagca gcuggucgag cacgagaacg ugccgaacgc caagcgcaag     3660

accaaggacc ugguguucca gcggaucgag cgcgccugcg uccccaagua ccagcucauc     3720

ugcaucuccc agugcagcgg ggacuccgac cggagcgugu ccgacuacua cuacaucacg     3780

ggcaagaagg aggugcgcaa cgcccugcag cuggcguucc ccaugcaccu cuacggggcc     3840

aucaacgcca ucgaggacaa gaaggagcug gacgacagcg cccuggagga gcucgucgug     3900

uacauccugc ggaacgcccc gcgcaucgcg cccgacgugu uccggcgccu gaaccucacc     3960

gagaagcugg ccgacugccu ggacaucccc gaccggaucg agcuccagaa cuccaucuac     4020

ggccuguuca accugaucaa cgccgagagc aacgucguga accuccagau cuucggcggg     4080

gccaagaccg ccggcgugaa gcugaucaac cugaacuccc uccugaacga ccccgcgacc     4140

acggucaccu ucaucgacca gagcgugacc gggauguucg agcgcaagga gaccaucggc     4200

aucuccuga                                                             4209


<210>  2136
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2136
augggcaagc aggaguucaa cgacuacuac cugggguucg acaucggcac caacagcgug       60

gggugggccg ucaccgacca gcaguacaag cuccugcgcu ucaacaagaa ggacaugugg      120

ggcucccggc uguucgagga ggccaagacg gcgaaggagc gccgggugua ccgcaacagc      180

cggcgccggc ucgaccgccg gaagcgccgg cugaagcugc ucgaggagcu guuccuggac      240

gagauccuca agguggacuc caccuucuuc aagcgccugg aggagagcaa ccuguacaac      300

gaggacaagu ccaucaaguu caacuacccc cucuucaacg acaaggacua caccgacauc      360

gacuucuaca aggaguacaa gaccaucuac caccuccgga aggcccuggu cuucgaggac      420

cgcaaguucg acauccggcu ccuguucauc gcccugcacc acauccucaa guaccgcggg      480

cacuucaucu ucgagggcca gagcuucgag gacaucaagu ccuucgacua caucuucacg      540

gagauccagg acuggauccu ggagaacuuc gagaucaaca ucgacaucaa ccugaaggag      600

aagcucgagg agaucauccu gagcaacaac accaagucca ucaagaagau cgaguucaag      660

aagaucuuca gcgacaaccg gauccuggcc auccucaaca ugggcguggg gugcaaggug      720

gaccugaagg acgucuucaa caacgaggag cugaaggacc agaagaucuc cuucaccgag      780

aucgacuacg agaacgagaa gcgcgacgag uucuaccagc uccugggcga ggggaucuuc      840

aucguggacc uggccaagaa gaucuacgac uucauggugc ucaagaagau ccugaagaac      900

agcgacuacu ucuccgagag caaggucgcg cuguacgagg agcacaagga ggaccucaac      960

cggcugaagu acaucuucaa gaaguaccug aaguccagcg acuacuacaa guucuuccgc     1020

uccaacagcg agaagaccaa cuacccgucc uacaucggcc ucaacaagau caagacgaag     1080

cggcugaucg agaacaacaa gugcagcauc agcgacauca acaaggagcu gaagaacaag     1140

uaccucaagg acauccaggu ggaggccgag gacgaggagc uguucaaccg cauccugcac     1200

aagaucaacg agaacaaguu ccucgagaag cugaagucca gcgagaacuc caccgugccc     1260

uaccaggucc acgagaucga gcugaagaag auccucgaca accagcgcaa guaccacagc     1320

uuccugaacg agaaguccga cgggaugacc cugugcgaga agaucaucaa gcuguucgag     1380

uuccggaucc ccuacuacgu gggcccccug aacaccaacc cgaacaugaa caagaagaac     1440

aagaacacgu ggguggucaa gaaggaggag aacagcaagu ccagcaucac ccccuggaac     1500

uucgagaaga agguggacga gcagaagucc gccgaggagu ucaucaagcg caugaccaac     1560

cggugcaccu accugcgcgg cgaggacgug auccccaaga acagccugcu cuacgaggag     1620

uucguccugc ugaacgagcu caacaaggug acgguggaca acgagcugcu gcaccccacc     1680

cucaagaacg agaucaucga cgagcuguuc aagaccaccg ucaaggugac gaagaagaag     1740

aucaccgagu uccugaagaa gaagcaggcc aucuccgacg uggagagcau caccgggauc     1800

gacgucgagu ucaacggcaa gcuccagggg uacaacaccu uccggaagau ccugggcgag     1860

aagaucgaga accugaacau caagaaguuc guggagcgcu gcaucgagcu caagugccug     1920

uacggcuccg acaagaagcu guucaagaac gcggucggga agcucuacaa ggaguacaac     1980

gugacggacg aggaguucac caagaucaac aagcugaacu ucaacaagug gggccggcug     2040

agcaaggagu uccucaccgg gauccgcuuc gagaagaccg acggcacgga gcgguucgag     2100

uccgugaucg ccgcccugcg cagcaccaac uacaaccuga uggagcuccu guccgacaag     2160

uucaaccugc agagcaagau cgacgagauc aacggggagg acgacuucuc caacaucgac     2220

aucaaggagu acgucaacga gcucuacgug agcccgagcg ugaagcgguc caucauccag     2280

accuacaaga ucgccaugga gaucaagaag aucaccggca agaaccccaa gaaggucuuc     2340

aucgagaugg ccaagggcgg gggcgagaag gggaagcgca cggagcggcg caaggacaag     2400

aucaaggagc uguacaagaa caugaaggac agccuguccg acuacugcaa caaccuccag     2460

gaccugagcu cccaggugga gaaguacgac gacaaccggc ugcgccagaa gaagcuguuc     2520

cuguacuuca ugcagauggg caagagcaug uacaccggga acgacaucga ccuguccgag     2580

cuguucaaca acaacaucua cgacaucgac cacaucuacc cccagagcaa ggugaaggac     2640

gacuccuucg acaacaucgu ccugguggag aagcaggaga accacagcaa guccaacgag     2700

cugcucaagc ccgagaucca gagcaagaug aacaacuacu ggaucuuccu gcgggagaac     2760

aaguucaucu ccaaggagaa guacaaccgc cugacccgga agaagaacuu caccgacgag     2820

gagcucggcg acuucguggc gcgccagcug gucacgaccc gccagagcac caaggaggug     2880

gccaagcugu ucgagaagau cuucgacggc accgaggugg ucuacuccaa ggccagccuc     2940

guguccgacu uccgggagcg cuucgacauc aucaaggugc gggaggucaa cgacgcccac     3000

cacgcccagg acgcguaccu gaacaucgug guggggaacg ucuucaacac caaguucacg     3060

aagaacuacu ggaaguucau caaggagaac aaccgcaaga acgaggagaa cccgaacaag     3120

aaggagggcu acaacuucaa gaacaucuac gagagcgacg uguuccggaa cgaggacgug     3180

gccugggucu ucagcaagga gaacaaggag aucaacacca ucaagaccgu gaacaagaug     3240

guguaccgca agaccgucaa caucacggag augcuggugg aggggaaggg ccagcuguuc     3300

aaccucacca ucaaggaccg gacccagcug aagaagaagg uggggaacau cgucgccaac     3360

auccccaagu cccucaagaa ccgcaacacc aagcucaaga gcgacgagcu ggccaagaag     3420

uacggcuacu ucgacucccu caacaacagc uacuucgugc uguucaagua cguggagaag     3480

gagaaggaga agaucggcuu cgacgggauc acgaucgucg acaaggagaa gcugaagaag     3540

aacuccgaca ucgagcgguu ccucgagaac aagggcaaca agaacgccaa gcugauccgc     3600

aagaucuuca agaaccagaa ggugcugauc gacggguacc ccuaccuccu caagagcugc     3660

aacaaggaca aggcgauccg guucgacaac uucaaguccc uguaccucga cgagaacgac     3720

cugaaguuca cccgcgacgc caucaaguuc gagaagaaca acgccgacau cgaccugaag     3780

aagcagaagu acaucaucag cuuccucaag aagaagaagc agaacgagau caacgagacc     3840

uacgaggagu gcaucgagcg gcugaacaac gaguucgaca cccuguuccu caaccugugc     3900

gacauccaga agucccccau cuacaagaac uacaagcugc cggacuucau cagcaagaag     3960

aacguggucg aggugaagga caaguucgag guguucaaga accucgagcu guacaacaag     4020

uccaagcuga ucaagaacuu ccucaacuac uucaaguacg acaacaacaa gcuggaccug     4080

aucgccaucg gcaucgacag caaggccaac ugcauccaga agcacuucac ggaggaccuc     4140

aucaaggacu ucgucaucgu ggacgagucc gugaccggcc uguucgcgaa gaaggagcgc     4200

cuggaguga                                                             4209


<210>  2137
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2137
augaccuacc gcaacuacca caucggccug gacaucggga ccagcuccgu gggcuucgcc       60

gugcgguacg acaacgggga gcucgugcac aagaagggca gcaaccuggu gggggcccgc      120

cuguucaagg agggcgagac ggcggaggag cggcgccuca accggggcgc ccgccggcgc      180

uacaaccggc gcaaguggcg gcugaaccug cucaaccaga ucuucaagcc ccugaucgag      240

gucaacgacc cgaccuucuu caagcgccug gagcagucca accucgugaa cuacgacaag      300

agcgaguccu ucagccugcu gaacaaccag uccgacgaca agcuccacca gcaguucccc      360

accaucuacc accuccggga cuaccugaug aaguccaccg agaaggccga cauccgccuc      420

aucuaccugg ccgugcacca caucgucaag uaccggggga acuuccugga cgccagcccc      480

guggacagcu ucgaggcguc caaccucaag guggacgaga uccugacgca gaucaaccug      540

cuccuggaca accugaccga cgaccuccgc cugaacaccc aguacgucac cgacaucgag      600

cagacgcugc ucagcaacac cgaguccaac aaggacaagg agaaggugau ccagaagcug      660

cugcuccaca aggaggccau caagaucgac aaggaccgga ccgcccagau cgccaagcug      720

gugcugggcu acaaggcgga caucgccaag cuguucggga ucaccgagaa gcugcagacg      780

cccaagcugg agcucagcgc cgccaacucc gacgacgccc ugauggagcu gagcgcggag      840

cucaacgagg accagaucgg cauccugcug cagcucaaga cccuguacuc ccgcguccgg      900

cugaaccaga ucaucccgag cgggaccucc cucagcaaga gcaugaucga gaaguacgag      960

cugcaccaca cccagcugaa guccuucaag gagaacaucc uccugaagcu gcgccggaac     1020

acggccgugc gccgcaaccu ggagcucgcc uacgcccugu acgugggcaa cgcccagacc     1080

aagcaggagg ucuucgagcc caagaaggcg cgguucacca gcgacgacuu cgccucccug     1140

aaggccaacg ccaagagcgc cuccagcgug aaggaggcgc ccaagaacac caagacgaag     1200

cucaccaccg acgacuucua caacauccug accgagcugu ucgccacguu cgaguccgag     1260

cucagcgccg acgaggacau gaucaaggag guggagcaga ucaagaaggc caucaccggc     1320

gacaacuacc ugcccaagca gcgcaccgac cagaacggga acaucccgca ccagcugcac     1380

cagaucgagc uccggcagau ccugcagaac cagcugcccc acuaccccga gauggacaag     1440

aucaacgagc agaagcccau cgagaagcuc gucaccuucc gcgugccgua cuacaucggc     1500

ccccugauca cgcccgagga ccagcagaag uccagcgccg cgaacuucgc cuggaugaag     1560

cggaaggagc agggggccau cacccccugg aacuucgacg acaaggugga ccgcaugcag     1620

uccgccacca acuucaucaa gcggaugacc gucaaggaca cguaccugau caacgaggac     1680

gugcucccgg accgcagccu gcuguaccag cgguucaagg ugcucaacga gcugaaccug     1740

gucaagguga acaaccagcu ccuguccauc ggcgagaagc aggcgaucua ccacgaccug     1800

uucgagaagc agaagaccgu cagcaccaag aagcuccagu ccuaccugca gagcgagcag     1860

ggcuacgggg acaacaucga gaucuccggc cugagcaacc ccgacaaguu caacaacggg     1920

cucaccacgu acaucgacuu caagaacauc uucggcgacc ugguggagga caccaccaag     1980

cagaaggagu ucgagcagau caucgagugg uccaccgugu ucgaggaccg cgccaucuuc     2040

aaggccaagc ugcaggaggu caacuggcuc accgaggagc agaucaaggc ccugguggcc     2100

aagcgguaca gcgggugggg ccgccugagc uaccggcucc uggcggagcu ggugaacgac     2160

aacggcgagc gcaucaucga cauccucugg aacaacaagg gguccaaccu gaugagcauc     2220

gucucccagg acgaguucca gcagcagauc gcccggauca acagccacga ggugcaggag     2280

cugggcaugg aguccgugcu cgccaacgcc uacacgagcc cccagaacaa gaaggccauc     2340

cgccagguca ugaagguggu ggacgacauc cagcuggcga ugaacgggca ggcccccacc     2400

uccaucagca ucgaguucac ccggaagccg gaggagaacu ccgagaucag caagucccgc     2460

uacgagacca ucacgagcau cuaccagucc cugagcaagg acaucgagaa ggaggucaag     2520

gacaaccuca acaagaacaa guccagccug aagaagaacc uguccgacaa guacuaccuc     2580

uacuucaccc agcugggccg ggacaucuac accaaccagc ccauccccuu caacgagauc     2640

cccuacuacg acaucgacca caucgugccg cgcagcgugg agaugaacga cagccugaac     2700

aaccgcgucc ucguggccaa guccaucaac cagaucaaga agaacaacgc ccccaucgac     2760

agcucccuga agaucaccag cgagacgcgg cagcugugga agaagcuccu ggacuccggg     2820

cugaucacca agcagaagua cuacaagcuc agcuccagca ucaagaacac cguguccccc     2880

uacgucaagc agggcuucau caaccgccag cugguggaga ccagccaggu gaucaagcug     2940

gucgccaaca uccucggcac gaaguaccag aacgacgaca ccgagaucau cgaggugaag     3000

gcgcaccuga acuccgagau ccggaagacc uucgaguucu acaagcugcg cgagcucaac     3060

gacuaccacc acggguucga cgccuaccug accacgcugg ugggcgggua ccucuacaac     3120

cgguacccca agcugcgcag cuacuucguc uacggcgacc acaagcggcu ggacaaggcc     3180

cagcucuccg agcugaagaa cuucaacuuc cugcacgggc ucaccaaccc caagagcggc     3240

uccgacggcc ugaucaccgc cagcgggucc cagcaggucg ugcuggaccg caacgagacc     3300

aucaagcggc ucaacaagau cuaccgcuac aaguacaugc ugaucagccg ggagguguuc     3360

acgaagcagg gccaguucua caacgagacc aucuacccgg cgaagcccaa caccgccaag     3420

augaucccgc ugaaggacaa caagcccucc gagguguacg ggggcuacag cagcuccaac     3480

gugagcaaga ucgcccucau caaggagaag aacaccgaca acuaccaguu caucggcauc     3540

cccauccgcu ccaucgccaa gcugaagcag gccaaggcga acagcaagac ggucuaccag     3600

accgagcuga accgggugau caaguccgug cucgccagcc agaagaccaa gaagcagaag     3660

gaguucgagg ucauccugga caacgugaag uacaaccagg uggucgugga ccagggggag     3720

aaguacuacc ugggcucccc cuacuacaag aagaacgcca agcagcucgu gaucagcccg     3780

gaguccguca agauccuggc cgacaaggcc accgcggacc ugcccgagca ggaggcccac     3840

aacaagcuca ugacggugua ccaggagauc guggacgucc ugaacaagca cuucgcccug     3900

uacgacauca acaacgugcg caagaagcuc aacgacgggc ugaucaaguu cagccagcug     3960

cccaacuacg acgagaccuc caagaaccgg uucgacauca gcaaguccau caagaccaag     4020

uacgccguga ucaugcgcau ccucagcggc aucaagaacc agaccuccga gguccccgag     4080

cuggggcuca agagcccgcu gaacaagaug cagacgaagg gcggcaucaa gaucuccccc     4140

gaggccaaga ucuacuacag cucccccacc gggaucuuca gccacuucaa gcaggucaag     4200

aaccuguga                                                             4209


<210>  2138
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2138
auggagcgca uccugggccu cgaccugggg gugagcucca ucggcugggc ccuggucgag       60

gaggacguga agaaccccga gaacaacaag aucaucaagc ucggggugcg ggucaacccg      120

cugaccaucg acgagaagac caacuucgag aagggcaaga gcaucacgac caacgccggg      180

cgcaccuccg cgcggagcgc ccgccggaac cugcagcgcu ucaagcuccg gcgcaagaag      240

cugcuggaga uccucaucca cuacaagauc aucaagaacg acgccgugcu ggccgagacc      300

ggcaagaaca cgaccuucca gacccaguac cugcgggcca aggcggcccg cgagaagauc      360

gagcucgacg agcuggcccg ggugcugcuc cugaucaaca agaagcgcgg cuacaagucc      420

agccggaagg uccagaacga cgacgacggg aaggccaucg acggccuguc cguggcgaag      480

gagcucuacg agaagaacua cacccccggg caguacgccu acgaccugcu gaagcgcggc      540

aagaagcaga uccccgacuu cuaccggagc gaccuccaga acgagcugga ccgcaucugg      600

gaguuccaga agcaguacca ccccgacauc cugacggacg aguucaagaa ggagcucgag      660

gggaagggcc agcgggccac cgccgccauc uuccugaacc gcuacaagau cuacaccgcg      720

gacaacaagg gcacccggga ggagaagaag auccaggccu accaguggcg cguggaggcc      780

cugucccagc cgcucgacgu caaggaggug gccuacguga ucgccgagau caacaacaac      840

cugaacaaca gcuccgggua ccugggcgcg aucagcgacc gguccaagaa gcucuacuuc      900

aacgggcaga cgaucggcga guaccuguuc gaggagcuga gcgccaaccc ccacacccgc      960

cucaagaacc aggucuucua ccgccaggac uaccaggacg aguucgagaa gaucuggaag     1020

auccagacca aguuccaccc cgagcugacc aaggagcuga aggagcaggu ccgggacauc     1080

gugaucuucu accagcgcaa gcucaagucc cagaagggcc ugaucagcuu cugcgaguuc     1140

gagucccggg agaucgagau cgaggagaac gggaagcgca agcggaagaa ggugggccug     1200

aagguggccc ccaagagcuc cccgcuguuc caggaguuca agaucuggca gguccucaac     1260

aacaucgaga ucaagaacga ggacgagcgc cgguaccugg agcccgagga gaaggagcuc     1320

cuguucaacg agcugaacug caaggggaac cucacgagcc agaagugccu ggagcugcuc     1380

ggcuucaaga gcaaggacgc caagcugaac uucaaggugg uggaggggaa ccgcaccaac     1440

gagaagcugu acgacgccuu ccugaagauc cucgagcugc agggcuacaa cguguucgag     1500

cugcucaaga ucaaggagga caaggacgag gcgaagcugu ccgagcugaa ggccagcgcc     1560

gacgagauca agggcaugau caaggagauc uucaacgcca accucaucaa caccuccauc     1620

cuggacuuca acgccgagcu ggaggggaag gacuucgaga accaggcgag cuaccaguuc     1680

uggcaccuca uguacuccua cgagggcgac aacagccccu ccgggaacga gaagcuguac     1740

gagcugcucg agaagaaguu cggcuucaag aaggagcaca gcaagauccu guccaccauc     1800

gucuucagcc aggaguacgg gucccugagc acgaaggcca ugcggcgcau cuaccccuac     1860

aucaaggaca acaaguacuc cgaggccugc cuccuggccg gcuacaacca cagcaagcac     1920

ucccugacca aggaggagcu cgagaaccgg aagcugaagg agcagcugga ggugcucccg     1980

aagaacagcc ugcgcaaccc cguggucgag aagauccuga accagaugau caaccucgug     2040

aacgcgacca ucuccgagua cggcaagcug gacggggucc ggaucgagcu ggcccgcgag     2100

cucaagaagu ccgcccagga gcgggaggac auggccaaga gcaucaacga ggccacccug     2160

cugcaccaga aguacgcgac gauccucaag caggaguucg gcguggugaa ccccagccgc     2220

aacgacauca uccgguacaa gcuguacaug gagcugaccg ucaacgggua caaggaccuc     2280

uacaccaaca ccaagaucga gaaggagaac cuguucacgg acaaguacga caucgaccac     2340

aucauccccc agagccgcuu cuucgacgac uccuucagca acaaggugcu ggugccgcgg     2400

caguccaacc ucgacaaggg caaccugacc gccuacgacu ucaugagcaa caaggggucc     2460

gagcgcgagg agcacuuccu gaacgucauc aaggagcucc uggacaaggg cagcaucucc     2520

aaggccaagu acgagaagcu gaagaagaag ggccucgaga ucggggacgg cuucaucgag     2580

cgcgaccugc gggacaccca guacaucgcc aagaaggcca aggagauccu guucgagauc     2640

accaacagcg uggugacgac cuccgggcgc aucaccgaca agcuccggaa ggacugggac     2700

cuggucaaca ucaugaagga gcugaaccuc gacaacuacc gccggcuggg ccugaccgag     2760

aucgugcgca acagcaaggg cgaggagaag gagcucaucc ccgacuggac gaagcggaac     2820

gaccaccgcc accacgcgau ggacgcccug accguggccu ucaccacccg gaaccacauc     2880

caguaccuga acuaccucaa cgcccgcaag gacgagaagc acaaggagca ccagaacauc     2940

uacgccaucg agaacaucau caccgagguc aucgagaaga agaacggguc caagacgcgg     3000

cgcuucaagg cgcccaugaa cagcuuccgg cacgaggcca agcagcaccu gaaggagauc     3060

cugguguccc acaagaccaa gaacaaggug gucaccagca acaucaacaa gaccaagaag     3120

aagggcggga uccacaagaa gacggagcuc acgccccgcg gccagcugca caaggagacc     3180

guguacgggu ccaagaaguu ccugcagagc cgggaggaga agguguccgc caaguucgac     3240

uacgagacca uccucauggu cgugaacccg auccaccgca acgcccugcu gaaccggcuc     3300

aaggaguacg gcaacgaccc caagaaggcg uucagcggca agaacgccau caacaagaac     3360

cccguguacc uggacgacag caagaccgac auccuguccg agaaggucac ccucagcugg     3420

uacgagaccg gguacaccau ccgcaagucc gugacgcccg acaacuucaa ggacuacaag     3480

aaccuggaga agaucaccga ccugggcauc aagaagaucc ucaaggagcg gcuggaccag     3540

uucaagggga acgccaagga ggccuucagc aaccuggaca aggacccgau cuggcucaac     3600

gaggagaagg gcaucgccau caagaccgug accaucacgg gggucuccaa cgcggagagc     3660

cugcacuuca agaaggacca ccugggcaag gagauccucg acgagaacgg caacaagauc     3720

cccggggacu ucguguccac cggcaacaac caccaccugg ccaucuaccu ggacgaggag     3780

gagaagcucg acgacaagau ggugaccuuc uacgaggccg uccugcgcgu gaaccagggg     3840

cugcccguga ucgacaagga cuacaacaag gagaagggcu acaaguuccu caugacccug     3900

aagcagaacg agauguucgu cuuccccaac gaggacuucg acccgaacga gaucgaccug     3960

cucgacgagc ggaaccugga gcgcaucagc aagcaccugu uccgcgugca gaagaucucc     4020

aagguggggu acggcaacag cuucguccgg gacuucgugu uccgccacca ccucgagacg     4080

accguggagg agcggaagga gcugcgcaac aucaccuaca uccagcugaa gucccucgag     4140

ggccugcgga acaucaucaa gguccgccug aaccaccucg ggaagaucgu gcagaucggc     4200

gaguacuga                                                             4209


<210>  2139
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2139
augaccaaga acauccuggg ccucgaccug ggggugagcu ccaucggcug ggcccugguc       60

caggaggaca gcaagaaccc cgagaacaac cgcaucguga agcucggggu gcgggucaac      120

ccgcugaccg uggacgagca gaucaacuuc gagaagggca agcccaucac gaccaacgcc      180

ucccgcaccc uggcgcggag cgcccgccgg aaccuccagc gcuucaaguu ccggcgcgcc      240

aaccugaucg acgugcugaa gaagugcaac auccucaagc agggggaccu gcugaccgag      300

aucggcaaga acuccacguu ccagacccag gagcuccggg ccaagagcgc caaggagaag      360

gucgagcugu ccgacuucgu gcgcgugcug cuccugauga acaagaagcg gggcuaccgc      420

agcucccgga aggcgaagaa cgaggacgag gggcagauca ucgacggcau gagcgucgcc      480

aagaagcugu acgaggagaa ccucaccccc ggggaguacu ccuaccagcu gcugaucgag      540

aacaagaagc agcuccccga cuucuaccgc agcgaccugc aguccgaguu cgaccgggug      600

uggaaguucc agaagcaguu caacccggag auccugaccg acgaccucua caaggagcug      660

cagggcaaga acaagaacgc cacgugggcc gugcugaagg agcccuucag ccucgucggg      720

aucaagcaga ucggcaccau ccaggagaag aaggcggaga aguacuucug gcgcuccgag      780

gccgugaagc ggcagcugga cuucgagagc cuggccgugg ucuuccagga gaucaacucc      840

aaccucaaca acagcuccgg cuaccugggg gccaucagcg accgcagcaa ggagcuguac      900

uucaacaacc agaccguggg cgaguaccuc uuccagcagc ugaaggagaa cucccacacc      960

aagcugaaga accagguguu cuaccggcag gacuaccucg acgaguucga gaagaucugg     1020

gagacgcaga gcaaguacua caaggagcug accaaggagc ugaaggagga gauccgcgac     1080

aucaucaucu ucuaccagcg gaagcucaag ucccagaagg ggcugaucag caucugcgag     1140

uucgagaacc gcgagaucga gaucaccgag aacggcaaga ucaagaagaa gaccgucggc     1200

cugaaggugg cccccaaguc cagcccccuc uuccaggagu ucaagaucug gcagguccug     1260

aacaaccugg uguuccaguc caucgagagc aaggagcugu ucccgcugga ccuggacauc     1320

aaggagucca ucuucaacga ggugaacauc aaggggaacc ucagcgcgaa gaacgugcug     1380

gaccugaugg gcuacuccgg gaaggagugg aagacgaacu ucaagaacau cgagggcaac     1440

cgcaccaacg agaaccugua caccgccuuc cucaagauca ucgccagcga ggggaucgag     1500

uuccccaagg aguucaagcu guccaucgac gacgacauca aggucagcaa gaucgacauc     1560

uccagcgaga agaucaagga guucaucaag gagaacuucc ugaagcucgg caucaacacc     1620

uccauccugg acuucaaccc cgagcuggac ggcaaggacu ucgagaagca ggagagcuac     1680

cagcucuggc accuccugua cuccuacgag ggggacgaca gcgccagcgg caacgugaag     1740

cucuacgagc ugcuggagaa gaaguucggg uucaagaagg agcacuccaa gauccucgcc     1800

gacgucggcu ucagccccga cuacgggucc cugagcucca aggcgaugcg gaagaucuac     1860

ccguacauca aggagaacaa guacagcgac gccugccacc uggccggcua caaccacucc     1920

aagaacagcu ucacgaagga ccagcucgcc aaccgcaucc ugaaggagca gcuggagauc     1980

cuccccaaga acgcccugcg gaaccccgug guggagaaga uccugaacca gaugaucaac     2040

cucgucaacg aggugaucaa ggcguacggc aagcccgacg agauccgcau cgagcuggcc     2100

cgggagcuga agaagaacgc cgaggagcgc gccaacauga ccuccgacau cagcaaggcc     2160

acccuccugc accagaagua cgcggaggug cugcagaagg aguucgggau caaggccccg     2220

ucccggaacg acaucauccg cuacaagcuc uaccuggagc uggagaagaa ccucuucaag     2280

gaccuguaca ccgacgucca gaucaagcgg gaggagcugu ucacggacaa guacgacauc     2340

gaccacauca ucccccagag ccgcuucuuc gacgacuccu ucagcaacaa ggugcucgug     2400

ccccggggcg ccaaccugaa gaaggggaac uucaccgccc uggacuacau ggagaucgag     2460

ggcaaggaga agcucgagaa guuccugaac aucaucaagg agcuguucga caagggggcg     2520

aucuccaagg ccaaguucga gaagcuccag aagaagggca gcgagaucgg cgagggguuc     2580

aucgagcgcg accugcggga cacccaguac aucgccaaga aggccaagga gauccuguuc     2640

ggcaucacca acuccgucau cagcacgagc gggcgcauca ccgacaagcu ccgggaggac     2700

uggaaccugg ugaacaccau gaaggagcug aaccucgaga aguaccgcaa gcugggccug     2760

accgagcuca ucgugaacuc caagggcgag gagaaggagc ggaucaucga cuggacgaag     2820

cgcaacgacc accggcacca cgccauggac gcgcugaccg ucgccuucac cacccacaac     2880

cacauccagu accugaacua ccucaacgcc cgcaaggacg agaagcacaa ggagcaccag     2940

aacaucuucg ccaucgagaa cacgaucacc gagaucgugg agaagaagaa cgggagcaag     3000

gagcgccggu ucaagcugcc caucaagaac uuccgcaccg aggccaagaa gcagcuggag     3060

gaggugcuca ucucccacaa ggcgaagaac aaggucguga ccaagaacau caacaagauc     3120

aagaagaagg gcagcaucau cgccaagacc gagcugacgc cgcgggggca gcugcacaag     3180

gagaccaucu acggcuccag caaguuccuc aagaccaagg aggagaagau cuccgggaag     3240

uucgaccugg agaugaucca gaaggugcag aacgagaagu accgcaacgc ccugcucaag     3300

cggcugcagg aguucggcgg cgacagcaag aaggccuuca ccgggaagaa cgucaucucc     3360

aagaacccca ucuuccugaa cgccgagaag acggagcagc uccccgagac cgugacccug     3420

gcgugguacg agaccggcua cacgauccgc aaggugguca accccgacaa cuucaaggac     3480

uucaagaaca ucgagaaggu gaucgacaag ggggugcggg acauccugac cgagcgccuc     3540

aaggaguuca acggcaacag caaggaggcc uucuccaacc uggagaagaa cccgaucugg     3600

cugaacaaga gcaaggggau cuccaucaag accgucacca ucacgggcau caacaacgcc     3660

gaggcccucc acuacaagaa ggaccaccug ggcaaggaga uccuggacga gaacgggcag     3720

cggaucgcgg uggacuucgu gagcaccggc aacaaccacc acgucgccau cuacgaggac     3780

gacaacggga accuccagga gaagguggug uccuucuacg aggccgucga gcgcgugaac     3840

cagaaccugc ccaucaucaa caaggaguac aacuucgagc ugggcuggaa guuccucuuc     3900

accaugaagc agaacgagau guuccuguuc cccagcgacg acuucgaccc caaggaggug     3960

gaccuguucg acgacaagua ccucucccug aucagcaaga accuguuccg gguccagucc     4020

aucagcaucg ugcaguacgg gaacagcgug auccgcgacu ucaaguuccg gcaccaccuc     4080

gagaccuccg ucagcgacga gaagaagcug cagggcguga cguaccagca gaucaagucc     4140

cugcagccgc ucaaggacau caucaagauc cgccugaaca accugggcaa gaucgugcag     4200

aucggggagu acuga                                                      4215


<210>  2140
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2140
augaagggca acaucgagaa gaagaagcag gaguucaacg acuacuaccu gggguucgac       60

aucggcacca acagcguggg gugggccguc uccgaccuca gcuacaaccu gcugcgcuuc      120

aacaagaagg acaugugggg cgcccgccuc uucgacgagg cgaagaccgc caaggaccgg      180

cgggugaagc gcaacucccg gcgccggcug gagcgccgga gcaagcgccu gaaguaccuc      240

gaggagaucu ucgagacgga gaucugcaag aucgacaaga acuucuuccg gcgccuggag      300

gaguccaccc ugcacaacga ggacaagacc uucgacuucc gguuccccau cuucaacgac      360

gagaacuaca ccgacaagga guacuacaag gacuucccga cgguguacca ccuccgcaac      420

gagcugauca agaacgagaa caagaaguuc gacauccggc ugaucuaccu cgccauccac      480

aacauccuga aguaccgcgg gcacuucauc uucgagggcc aggacuucga cagcgucacc      540

gacuucaagg agguguucuc cgagcugagc gccuaccucu ccgagaaccu ggaggagaac      600

cuggacauca accucugcga caacaucaag gacaucaucu gcagcaagga gaacaagacc      660

uccaagggca agagcuucgu gucccugauc gacagcaagg ucauccaguc cgugcugcgg      720

ugcugcaucg gguaccgcgu gaaguucaag gacaucuuca ucaacuacga ggagaaggag      780

aagaacgagg gcaucaaccu cagcgagauc gaguacgacg agaagcggga cgagaucgcc      840

cugucccugg gggagaacau ccuccugauc gacaucugca agaaggucua cgacuucaug      900

auccugaaca acauccucaa gggcaacaag uacaucagcg aguccaaggu gaaccuguac      960

gacgagcaca agugcgaccu gugcaaccuc aaguacaucu ucaagaagua ccugcccaag     1020

aaggacuacu ucgaguucuu ccgcaacgag aacaaggaga agaacuacgu gagcuacauc     1080

agcuccagcc ugaacaacgg gaagaagaag aacacgugca cccaggagga ccucaacaag     1140

gagaucaagg aguacaucgc gaagaucaag gaguacgagg agaacgacgc cguccuggag     1200

aagaucaacc ugaagcucaa cgagaacaag cugcugccca agcucaagac caacgacaac     1260

ggcgugaucc ccuaccagau ccacaagauc gagcuggaga agauccugca gaaccagucc     1320

aaguacuacg acuuccuccu ggagaagauc gacggcauca ccaacaucga gcggaucgag     1380

aagcuguuca ccuuccgcau cccguacuac aucgggacgc ucaagggcaa guucagcugg     1440

gccaagcuga aggagaacaa gaagaucacc cccuggaacu ucgagaagau cguggacaug     1500

gaggaguccg ccaaggcguu caucgagcgc augagcaacc ggugcuccua ccugaagacc     1560

gagaacgucc uccccaagaa cagccugcug uacuccgagu acgagcugcu caacgagcug     1620

aacaagguga agaucaagaa cgaguucauc aacguggucc ugaagaacga gaucaucgac     1680

gagcuguuca agaccacgag caaggugacc aagaagaagu ucguggacua ccugaagacc     1740

aagcagaucu ucgucgagaa ggaggacauc uccgggcugg acaacgaguu caagagcaag     1800

cucgacuccu acaucaaguu caagaaggug aucggcgaca agaucgacac ccugcgcggc     1860

uacaacuucg uggagaagug caucgaguuc aagugcuucu acggggacga caagaagcug     1920

uucaagaacg ccaucaacaa cacggaguuc aaggacuacg ucagcgacga cgagcucaag     1980

aagaucaaca agaugaaguu caacaagugg ggccggcugu ccaagcgccu gcucaccgag     2040

aucugcuucg agaacgugau cgagagcgag uccuucgaga acaucaucgu cgcccugcgg     2100

agcaccaacu acaacuucau ggaguucacc uccaagaagu ucaaccugag cgagaaggug     2160

gccgaggaga accaggagaa cacgcucgag aaguucaaca ucaacgagua cguggacgag     2220

cuguacguca gccccuccgu gaagcgcagc aucauccaga ccuacaagau cgccauggag     2280

aucaagaaga ucaccgggaa gaacccgaag aagaucuuca ucgagauggc gcggggcggg     2340

ggcgagaagg gcaagaagac cgagaagcgc aaggagcgga ucaagacgcu guacgccaac     2400

augaaggacu ccaacgagga guuccucaac aaccuggaca accugaagcu cagcguggac     2460

aacuacgacg acaaccgccu gcgggagaag aagcuguucc ucuacuucau gcagcugggg     2520

aaguccaugu acaccggcaa gaacaucgac cugaacgagc uccuguucgc caacaccuac     2580

gacaucgacc acaucauccc ccagagcaag cugaaggacg acuccuucga caacaucguc     2640

cucguggaga agaccaucaa caaggacaag gacaaccgcg acuaccugga cgagagcauc     2700

cggcugcgca ugaacaaguu cuggaacuuc cuccgggaga agggguucau cacgaucgag     2760

aaguacaacc gccugcggcg caacaccuac uucuccgacg acgagcugag cggcuucauc     2820

ucccggcagc ucgugaccac ccgccagagc acgcuggagg ucaagaagcu guucgaguuc     2880

aucuuccaga acaccgacgu ggugugcucc aaggccaacc ucgucagcga cuuccgcgag     2940

aaguacggga ucaucaaggu gcgggaccug aacgacgccc accacgcgca ggacgccuac     3000

cugaacaucg uggucggcaa cguguucgac accaaguuca ccaagaacua cuggaaguuc     3060

aucgaggacc gccggaaggu ggacaacauc cgcgagaccu acaacuucaa gaagaucuuc     3120

gacuacgacg ucucccgggg cgagaacgug gccuggcuca aggacuacag caucaacacg     3180

aucuccgaga ugaucaacaa gaagaccgcc aacaucaccc agaugaucac cgaggggaag     3240

ggccagcugu ucgaccugac gauccucaag aaguccaaga ucgacaagaa gcugaccgug     3300

auccccaaca cccccaagau ccucaagagc agccucaagg acagcugcaa gaucgccgag     3360

aaguacgggu acuucuccac ccugaugccg gcguacuucg ucguguucaa guacaucgac     3420

aagaacaagg agguguucgg cuucgaccgc aucuucaucu ucgaccggga gaagaucaac     3480

agcaacgagg acaucgagaa guaccuggag gacaaggggu acaacucccc caaguucauc     3540

cgcaagguca accucaagca gaagauccug cugaacggcu accccuaccg gaucgugggc     3600

uucacggaca acaagcucga gaucaagaac agcaagcagu ucuacuccga cggggagcac     3660

cugaaguaca ucaagcaccu gagcgccuuc uacaacaaca acaacaacgu gaagaacggc     3720

gaguaccgca ucaacuuccu caagaagacc uccgagaacg accggaacga gaccaccgac     3780

gaggccaagg agcgccugaa caucgaguuc aucaagcugu acgaguacuu cugcgagcgg     3840

cucaacagca agaucuacgc caacuacuac aacuccaaga aguacgacga gcugaucgag     3900

gucuucgacu gcuucaagaa ccugaaccuc cuggacaagg ccaagcugcu ccuggaguuc     3960

cugaagaucu ucaagaagga cacguacggg aacuucaagc uccuggagaa gcccgagcug     4020

gacaagaacg gcaagcagaa gcucgacaag aacggcaacc ugagcuucga ggggcugucc     4080

auggcguucg gcaagcucaa cgggaagaag uucaagcagc ugguggagaa ggacauccag     4140

uucguggacg agagcgucac cggccuguuc gagaagaagg aggggcucga gaaguggcag     4200

gacggcgagc aguga                                                      4215


<210>  2141
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2141
auggccaaga agauccuggg ccucgaccug ggggugagcu ccaucggcug ggccaucguc       60

gaggaggacg cggagaacgu gaugaacaac aagaucguga agcugggggu ccgcgugaac      120

ccccucaccg uggacgagca gaucaacuuc gagaagggca agccgaucac cacgaacgcc      180

gggcggaccc ugguccgcgg cgcccggcgc aaccugcagc gguucaagcu ccgccggcug      240

caccugaucg acauccucca gaagcgcaac auccugaagc aggccgaccu gcucgccgag      300

agcggccgga acuccaccuu ccagacccag gagauccgcg ccaagagcgc gaagcagaag      360

guggagcugu ccgagcuggc ccgggugcuc cugcugauca acaagaagcg cggguacaag      420

agcucccgga aggccaagaa cgaggacgag ggccagaucg ucgacgggau gagcguggcg      480

aagaagcucu acgaggagaa cacgaccccc ggcgaguacu ccuacgagcu gcucaagagc      540

gggaagaagc agcucuccga cuucuaccgc agcgaccugc aggccgaguu cgaccgggug      600

uggaacuucc agaagcaguu cuaccccgag aucuucuccg acaaccugua caaggagcuc      660

cagggcaaga acaagaacgc caccuggaag auccuggaga uccccuucag ccuggucggc      720

aucaagcagg ucgggaccac gcaggagaag aagaucgaga aguacuucug gcgcuccgag      780

gccgugaaga agcagcucga cuucgagagc cuggccgugg uguuccagga gaucaacucc      840

aaccugaaca acagcuccgg cuaccucggg gcgaucagcg accggagcaa ggagcuguac      900

uucaacaacc agaccguggg cgaguaccug cacugccagc ucaaggagaa cccgcacacc      960

aagcugaaga accaggucuu cuaccgccag gacuaccugg acgaguucga gaagaucugg     1020

gagauccagu ccaaguacca caccgugcuc acgaaggagc ugaaggagga gauccgggac     1080

gugaucaucu ucuaccagcg caagcucaag agccagaagg gccugaucuc caucugcgag     1140

uucgagaacc gggagaucga gaucaccgag aacgggaaga ucaagaagaa ggaggucggc     1200

cugaaggugg cccccaagag cucccugcug uuccaggagu ucaagaucug gcagguccug     1260

aacaaccugc aguuccagaa caucgagagc aaggagaucu ucccccucga ccuggacugc     1320

aaggagucca ucuucaacga ggugaacauc aaggggcgca ucagcgccaa ggccgugcug     1380

gacguccucg gcuacuccgg gaaggagugg aagaccaacu ucaaggacau cgagggcaac     1440

aacagcaacg agaaccugua caacgccuac cugaagauca ucgcgaacga gggcaucgag     1500

uuccccaagg aguucaagug caaccuggac gacgacauca acguguccaa gaucaucugc     1560

agcgccggga agaucaucga guucgugcgc gagaaguucu ccgagcucgg caucaacacc     1620

agcauccugg acuucaacuc cgagcuggag gggacgaacu ucgagaagca gaaguccuac     1680

cagcucuggc accuccugua cagcuacgac ggcgacgaca gcccguccgg gaaccagaag     1740

cuguacgagc ugcuggagaa gaaguucggc uucaagaagg agcacagcau cauccucgcc     1800

gacaucggcu ucccccagga guacgggagc cuguccacca aggccaugcg gaagaucauc     1860

ccccaccuga agaccggccu ccccuucgcc aagaaguccg acaacgacac cauccugagc     1920

gcgacggcca uggccgggua caaccacagc aagucccuga ccaccgagga gcucaccaag     1980

cgcguccuga aggagcaccu ggagauccuc ccgaagaaca gccugcggaa ccccguggug     2040

gagaagaucc ugaaccagau gaucaacguc gugaacgagg ugaucaagga guacggcaag     2100

cccgacgaga uccgcaucga gcucgcccgg gagcugaaga agaacgccga ggagcgcgcg     2160

aacaugacgu ccgagaucgg gaaggccacc auccagcacc agaaguacag cgagauccug     2220

cagaaggagu ucggcaucaa gucccccagc cggaacgaca ucauccgcua caagcucuac     2280

cuggagcugu ccaacaacgg cuucaaggac cucuacaccg acauccagau caagcgggag     2340

gagcuguuca ccgacaagua cgacaucgac cacaucaucc cgcagagccg cuucuucgac     2400

gacuccuuca gcaacaaggu ccuggugccc cgcggggcca accucaagaa gggcaaccug     2460

acggccuucg acuaccugga guccgagggg aaggacaccc ucgagaaguu caucaacacc     2520

aucaccgagu ucuacgacaa gggcaucauc acgaaggcga aguacgagaa gcugcagaag     2580

aagggcaccg agaucgggga cggcuucauc gagcgggacc ugcgggacac ccaguacauc     2640

gccaagaagg ccaaggagau ccucuucggg aucaccaaca gcguggucuc cacgagcggc     2700

cgcaucaccg acaagcugcg ggaggacugg aaccugguga acaccaugaa ggagaugaac     2760

cucgagaagu accgcaagcg ggggcugacc gagaccguga ucaacagcaa gggcgaggag     2820

aaggagaaga ucaucgacug guccaagcgc aacgaccacc ggcaccacgc cauggacgcc     2880

cugacggucg cguucaccac ccacaaccac auccaguacc ucaacuaccu gaacgcccgc     2940

aagaacgagg accacaagca gcacaaggug aucagcaaca ucgagaaccu gaucaccaag     3000

guguucgaga agaagaacgg cuccaagcag cgccgguucc ucgagcccau ccacaacuuc     3060

cgcaucgacg ccaagaagca ccuggaggag auccugauca gccacaaggc caagaacaag     3120

gucgugacga agaacaucaa caagaucaag aagaaggggu ccaucaucgc caagaucgag     3180

cucacgcccc ggggccagcu gcacaaggag accaucuacg ggagcuccaa guaccugaag     3240

accaaggacg agaagcucag cggcaaguuc gaccuggaga ccaucaacaa ggugcagaac     3300

gagcgcuaca agaacgcgcu gcucgagcgg cugaaggucu ucggguacga cuccaagaag     3360

gccuucaccg gcaagaacau ccuggccaag aacccgaucu uccucaacau cgagaagacc     3420

gagcagcugc ccgaggugcu gacccucgcc ugguacgaga cgaucuacac cauccgcaag     3480

agcaucaacu ccgacaacuu caaggacuac aagaacaucg acaaggugac cgacaagaag     3540

guccacaaca uccugaccga ccggcugcgc gaguucaacg gcaacagcaa ggaggccuuc     3600

uccgaccucg agaagaaccc caucuggcug aacaagccca aggggaucgc gaucaagacg     3660

gugaccauca ccggcgugaa caacgucgag gcccugcacu acaagaagga ccacuucggg     3720

aaggaguucc ucgacgagaa gggccacaag aucccggugg acuucgugag caccgggaac     3780

aaccaccacg ucgccaucua cgaggacacg gccggcaacc ugcaggagcg ggugcugucc     3840

uucuacgagg cgguggagcg cgucaaccag ggccucagcg ugaucgacaa ggaguacaac     3900

uccagccugg gguggaaguu ccuguucacc augaagcaga acgagauguu ccucuuccac     3960

uccgaggacu ucaaccccca ggagaucgac cuguacgacg agaagaaccu gcggcucauc     4020

agcaagaacc uguuccgcgu gcagaaguuc accgucaagg acuacuucuu ccggcaccac     4080

cuggagacca acguggagga caacagcaag cucaagggcg acacguggaa gcgcgagggg     4140

cugaacggca uccgggggau ccugaaggug cgccucaacc accugggcaa gaucguccag     4200

gugggcgagu acuga                                                      4215


<210>  2142
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2142
augagcaaga agauccuggg ccucgaccug gggaccaccu ccaucggcug ggccuucgug       60

cgcgaggccg aggacuucca ggaggagagc uccaucgagc ggaucggggu ccgcgugaac      120

ccccugacga ccgacgagca gaucaacuuc gagaagggca agccgaucag cgugaacgcg      180

gaccggaccc ucaagcgcgg ggcccggcgc aaccugcagc gguacaagca gcgccggacc      240

aaccugaucc agcuccugaa gcaguacaag aucaucaacg agaacacgau ccuggccgag      300

gacgacaaga acaccaccca cuccaccuac gcccuccgcg ccaaggcggu cagcgagaag      360

aucuccaagg aggccuucgc ccgggugcug cugaugauca acaagaagcg cggcuacaag      420

agcucccgga aggccaagaa cgaggacgag ggccaggcga ucgacgggau gagcguggcc      480

aagcgccucu acgaggagga caucacgccc ggccagcugu gccugcagcu ccugaaggag      540

gggaagaaga agcugcccga cuucuaccuc uccgaccugc aggccgagcu ggagaagauc      600

uggaacuucc agaagaaguu cuaccccgag auccucaccg acgaguucaa gaaggagauc      660

gagggcaagg ggcagcgggc caccagcgcc uccuucuggg cgaaguacaa guucaacacc      720

gccgagaaca agggcagccg cgacgagaag aagaugcagg ccugggagug gcgguccaag      780

gccaucaaca cgcagcugac ccgcgaggag gucgccuacg ugaucaccga gaucaacaac      840

aacaucaaca acagcuccgg cuaccugggg gcgaucagcg accgguccaa ggagcucuac      900

uucaacaagg agaccguggg cgaguaccug uuccgccagc ugcgguacag cccgcacacg      960

cgcgucaaga accagguguu cuaccgccag gacuaccucg acgaguucga gaccaucugg     1020

gagaaccaga gccgguacca caacgagcug accaacgagc ugaaguccga gauccgcgac     1080

guggucaucu ucuaccagcg gaagcucaag agccagaagg ggcugaucuc cuucugcgag     1140

uucgagagca agcagaucga gauccagaag gacggcaaga ucgugaagaa gaucaucggc     1200

cugaaggugu cccccaagag cagcccccuc uuccaggagu ucaagaucug gcagauccug     1260

cagaaccugg aguucaccaa caaggagacg aaggagaaga agguccccga cuucgaggcc     1320

aagaaccucc uguucgagga gcugaacauc aaggggaacc ucaagggcga gaaggccauc     1380

gagauccugg gguacaagcc gaaggagugg cuggugaacu acaaggagcu cgacggcaac     1440

cgcaccaacc aggugcugua cgagggguac cugaagaucc ucgaggucga gggcuacgac     1500

aucaaggacc ugcugaagau caaguccaac aaggacgagg ugaaccucuc cgagcugagc     1560

aagcccgugu ccgagaucaa ggaccugauc aagagcaucu ucgagucccu gaacaucaac     1620

accgccaucc ucgacuucaa ccccgagcug gagggcaagg acuucgagcg gcaggccagc     1680

uaccagcugu ggcaccuccu guacuccuac gagggggaca acagcaacac cggcaacgac     1740

gagcuguacc gccuccugua ccagaaguuc ggguucggca aggagcacgc gcagauccug     1800

gccaaccucu ccuucagcca ggacuacggg ucccugagcg ccaaggccau gcggaagauc     1860

uaccccuuca ucaaggagaa cagcuacucc gaggccugca gccuggcggg cuacaaccac     1920

uccagcucca ucacgaagga ggagaacgac aagcgcgagc ucaaggagaa gcuggagcug     1980

cucccgaaga acagccugcg gaaccccguc guggagaaga uccugaacca gauggugaac     2040

gucaucaacg ccaucaucgc cgaccccucc augggccgcc ccgacgagau ccggaucgag     2100

cucgcccgcg agcugaagaa gaacgcggcc gagcgggccg agaugaccau ccacaucaac     2160

aaggccaaga gcgagcacga caacuaccgc aagauccuga ccaccgaguu cgggaucccg     2220

aaccccacgc ggaacgacau cauccgcuac aagcucuacg aggagcugaa ggugaacggc     2280

uacaagaccc uguacuccaa caccuacauc ccccgggaga agcuguucag caaggaguuc     2340

gacgucgagc acaucauccc caaggcccuc cuguucgacg acuccuucag caacaagacc     2400

aucugcgcgc gccaggagaa ccucgacaag gggaacaaga cggccuucga cuucauccag     2460

uccuucuacg gcaaggagaa guucgaggag uaccugcagc ggaucgagag ccuggccaag     2520

aucaaggagg gggacaccuu cgugaucucc aaggccaagu acaagaagcu ccugcugccg     2580

gagaucgaga ucggcgaggg cuucaucgag cgcgaccucc gcaacagcca guacaucgcc     2640

aagaaggcga aguccaugcu gcuggacauc ugcaagaccg ugguccccac cacggggagc     2700

aucaccgacc ggcuccgcga ggacuggggc cugaucaaca ucaugcagga gcugaacuuc     2760

gagaaguaca agaagcucgg gcugaucgag augguggaga agaaggacgg cagcuucaag     2820

gagcggauca ccgacuggac caagcgcaac gaccaccggc accacgccau ggacgcccug     2880

accguggccu ucacgaagca caaccacauc caguaccuca acuaccugaa cgcccgcaag     2940

aacgagaacc acaagaugca cggcaacguc aucgcgaucg agcagaagga gaccgagcug     3000

cucguggacu ccguggggaa gcggaagcgc aaguucaagc ugcccaucag caacuuccgg     3060

gaggucgcca agaugcaccu ggagaacauc cucguguccu acaaggccaa gaacaaggug     3120

gccaccaaga acaagaaccg cuucaagacc aagaacggcg agaaggucaa gaucgagcug     3180

acgccccggg ggcagcugca caaggagacc guguacggca agaugaagaa guacgagacc     3240

aaggaggaga ucaucgggag caaguucgac gaggcgacca uccucaaggu ggccaaccug     3300

gagcagcgca acgcccugcu ccagcggcug aaggagaaca acaacgaccc gaagaaggcc     3360

uucuccggca agaacagccu gaaguccaac ccccucuacu acggcgucga cagcaagccc     3420

auccccgagc gcaucaagau cguguggcug gaggacgacu acacgauccg gaaggagauc     3480

accccggacc ugaagaucga caaggugauc gacaucggga uccgccggau ccucgagaac     3540

cgccuggccg aguucaacgg caacgcgaag gaggccuucu ccaaccugga gaagaacccc     3600

aucuggcuca acgaggggaa gcggaucgcc aucaagcgcg ucaccaucag cggcgugaag     3660

aacgccgaga cccugcacua caagaaggac caccuggggc acgagaugcu cgacaagcag     3720

ggcaagcgga uccccgugga cuucgucucc acgggcaaca accaccacgu ggccaucuac     3780

aaggacgaca agagcgaccu gcaggagaag guggucuccu ucuacgaggc gguggagcgc     3840

aucaaccagg agcugccgau caucgacaag agcuucaacc acgaccucgg guggcaguuc     3900

cuguucacca ugaagcagaa cgaguacuuc guguuccccc gcgacggcuu cgaccccuac     3960

gagaucgacc ugcucaaccc cgacaacuac aagaucaucu ccccgaaccu guuccggguc     4020

cagaagaucg ccaccaagaa cuacguguuc aaccaccacc uggagaccaa ggccguggac     4080

ggggagcucc ugaagacgaa gaaggaccug agcgacauca ccuaccgcuu cauccagacc     4140

cccgucaacc ucaagaacau caucaaggug cggaucaacc acaucggcca gaucgugaag     4200

aucggggagu acuga                                                      4215


<210>  2143
<211>  4218
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2143
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaag       60

gaggccgaga acuccaacga gaaguccgag aucaucaagc ucggggugcg cgugaacccc      120

cugacggugg acgagaagac caacuucgag gcgggccggc cgcugagcac caacgccgac      180

cgcaccgcca agcggagcgc ccgccggaac cuccagcgcu acaagcugcg gcgcaagaac      240

cugaucgacc uccugaucaa gaaccagcug aucgacaagg acacgccccu caccgagauc      300

gggaagaaca ccacccacca gacgcuggag cugcgggcca aggcggcccg cgagcggauc      360

gagcucgagg accuggcccg cgucuuccug gccaucaaca agaagcgggg cuaccgcucc      420

agccggaagg ugaacaacga ggaggagggc caggcggugg acgggauggc cgucgccaag      480

aagcucuacg acgagaaccu gacccccggc caguacgccu acgagcugcu cuccaagggg      540

aagaaguacg ugcccgacuu cuaccgcagc gaccugcagg ccgaguucaa cgcgaucugg      600

gaguaccaga agcaguucua cgccgacauc cuggacgacg agcucuacaa cgcccugaag      660

ggccaggggc agcagaacuc ccggaagcgc uuccuggcca ucaagggcgu guacaccgcc      720

gagaacaagg gcaagcggga cgacgucaag cuccgccacu acaaguggcg gagcgaggcg      780

aucuccaaga agaucaacau cgaggaggug gccuacgugc uggucgagau caacaacgac      840

cugaacaaga gcuccgggua ccucggcgcc aucagcgacc gcuccaagga gcuguacuuc      900

aacaaggaga ccguggggga gaaccugugg aagcagaucc agaagaaccc gcacacgagc      960

cucaagaacc agguguucua ccgccaggac uaccuggacg aguucgagca gaucugggag     1020

acccaggccc aguuccaccc ccagcugacc cucgcccuga aggagcagau ccgggacguc     1080

aucaucuucu accagcgcaa gcugaagucc cagaagggcc uccugagcuu cugccaguuc     1140

gaguccuggg agaucgagcg gaaggacgag aacggcaacg ugauccugaa caagaccacg     1200

cagcucccca agcgccagac cguggggcgg cgcgucgcgc ccaagagcag cccgcuguuc     1260

caggaguuca agaucuggca gaacaucaac aaccuggagg ugaccaagau cuccgagggc     1320

gccagcaaga acaagaagga gaccgagacg cucuccgacg acgagcggaa gcugcuguuc     1380

gaggagcuca accugcgcgg gaaccugagc cagaaggagg ugcugcagau ccucggccug     1440

aaggacaagg aguacaagac caacuucucc gaggggcugg agggcaaccg gaccaacgcc     1500

gcccucuuca acaucuacca gcagaucgcc gagaacgagg gcuacggguu cgacuggagc     1560

aagaaguccg cgcaggagau caaggaggag cugaaggccg ucuuccccca gaucggcauc     1620

caggccgaca uccuggacuu caacgccaac cucgacggga aggaguucga gaaccaggcg     1680

agcuaccagc uguggcaccu ccuguacucc gccgaggagg acgacaagau caacgaggag     1740

gaccagauca ucuacggcaa cagcgccgug ucccugaaga agaagcugug cgagaaguuc     1800

ggguucaccc ccgaguacgc caaguggauc gccaacguga gccuccagga cgacuacggc     1860

aaccugucca cgaaggcgau gcgcaagauc auccccuacc ugaucgacgg caacgacuac     1920

agcgaggccu gcgcccucgc cggguacaac cacuccaaca gccugaccaa ggaggagaac     1980

gacaaccggg agcugcucga caagcuggag cugcucccga agaacucccu gcgcaacccc     2040

gucguggaga agauccugaa ccagaugguc aacgugguga accaggucau cgagaccuac     2100

ggcaagcccg acgaggugcg gaucgagcuc gcccgcgagc ugaagaaguc cgcggagaag     2160

cgggccgaga ugaccaagag caucaacgag gccacgcgcc ggaacgagga caucaagaag     2220

cugaucacca aggacuucgg gauccccaac ccgaccaaga gcgacguggu ccgcuaccgg     2280

cucugggagg agcuggcgcc ccuggccuac aaggacgugu ucaccggcaa gcagaucaag     2340

aaggaggacc ucuucagcuc caagaucgac aucgagcaca ucauccccaa ggcccugcug     2400

uucgacgaca gcuucuccaa caagacgcuc guguuccgcg agaccaaccu gaagaaggcc     2460

gaccgcaccg ccuacgacuu caucgagagc gacuacaacg ccacccugga cgacuacauc     2520

cagcgggucg agacgcucua caacaacacc aaggggacca ucuccaaggg caagcgcaac     2580

aagcugcuga uggcgcagaa gaaccucccc gacggcuuca ucgagcggga ccugcgcaac     2640

agccaguaca ucgccaagaa ggccaaggcc augcuguacc agguguucaa ggacgugaac     2700

gucaccuccg ggagcaucac cgaccggcuc cgcgaggacu gggaccugau caacgugaug     2760

aaggagcuga acuucccgaa guacaaggcc cucggccuga cggagaucga ggagcgguac     2820

gacaucgggc aggagaagac caagcgcgug gaggucauca ccgacuggac caagcggaac     2880

gaccaccgcc accacgcgau gaacgcccug acgguggccu ucaccaccuc cagccacauc     2940

caguaccuca acuaccugaa cgcccggaag aacgaguccc acaaggagca caagaacauc     3000

aagggcaucg agaacaagau cacccacaag cagaaggaca agcgcaaguu caucccgccc     3060

augcccaacu uccgggagga ggccaagaag cacaucgaga cgauccuggu gagcaucaag     3120

aacaagaaca aggucuacac caagaacauc aacaagacca agaagaaguc cggccacaac     3180

accaaggagc agcucacgcc ccgcgggcag cugcacaagg agaccgugua cggcaagagc     3240

aagcggccca uguccaagcc caccaagauc aacaagaacu ucagccugga gcaggcgcag     3300

cucaucauca acgugcagga gaaggagcug guccugcgcc accucgccca guucgacaac     3360

aaccccgccg uggccuucga caccaagacg cugaagaaga ugccgcugcu caagaacggg     3420

gagccccuga aggaggugcu gugcuucgag gagaucuuca ccauccggaa ggacaucacc     3480

cccgaccuca agaucgagaa ggucguggac gagaagauca aggccauccu ggagcagcgc     3540

aagaaggacu ucggcgggaa cgcgaaggag gccuucuccg accuggacaa gaacccgauc     3600

uggcucaaca agaagaaggg caucugcauc aagcggguga ccaucacggg cgucagcaac     3660

gccgaggccc ugcaccacca gaaggaccac cuggggcgcg agauccucga cgacaacggc     3720

aaccccaucc ccgcggacuu cgugagcacc gggaacaacc accaccuggc cguguaccug     3780

gacuccgagg gcaagcucca ggacaacauc gucagcuucu acgaggccgu ggugcggacc     3840

ucccaggggc ugcccgucgu ggacaagggc cugaacaagg accucggcug gaaguuccug     3900

uucagccuga agcagaacga gauguucgug uucccgaacg acgacuucga caucaacgac     3960

uucgaccucc uggacgacaa gaaggccgag cugaucucca agcaccucua ccgcguccag     4020

aagaucagca agguguccgc cgggaacagc uucgugcgcg acaucgucuu ccggcaccac     4080

cuggagacca cggugaagga ggugaaggag cugaaggaca ucuccuacau ccagcucaag     4140

agccuggagg gccugcgcaa caucaagaag guccggauca accacaucgg gaagaucguc     4200

caggugggcg aguacuga                                                   4218


<210>  2144
<211>  4221
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2144
auggagaaga agaucaucgg caucgaccug ggggugacca gcaucggcug ggccuacguc       60

aucgaggacg agaacgggca cucccgcauc cuccggaucg gcacccgcgu gaacccccug      120

acgaccgagg agcugaagaa cuucgagcag gggaagccga ucaccgagac cgccgagcgg      180

acgcucaagc gcagcaugcg gcgcaaccug cagcgguaca agcagcgccg gaccaaccug      240

aucaacgugc uccugaagaa caagcugauc accgagacca cggcgcucgc cgaggacggc      300

aagggcacca cccaccugac ccacgcccug cgcgccaagg ccgcguacgc ccagaucccc      360

cucgaggacu ucgcccgggu ccugcugaug cucaacaaga agcgcgggua ccgguccagc      420

cgcaagguga agacggaggc cgacggccag gcgaucgacg ggauggccgu ggccaagaag      480

cuguacgagg agaacaucac ccccggccag cugugcuaca cccuccugcu gcaggggaag      540

aagacccucc ccgacuucua ccgguccgac cugcaggucg agauggagaa gguguggacg      600

uuccagaagc aguuccacca ggccauccug accgacgagu uccgcaagua caucgccggc      660

aagggccagc gggcgaccgc cgccagcuuc ugggggaagu acggcuucaa caccgccgag      720

uucaaggggu cccgcgagga gaagaagcuc aagagcuacg aguggcgguc ccaggccauc      780

acgaccccgc ugagccagga ggagguggcg gccgucauca ccgacaucaa cuccaacauc      840

gccaacagcu ccggcuaccu gggcgccauc agcgaccgcu ccaaggagcu guucuucaac      900

aagcagaccg uggggcagua ccaguacaac cugcugcagc agaaccccca cacgaaccuc      960

agcggccagg uguucuaccg gcaggacuac cuggacgagu ucgagaccau cugggagacc     1020

caggccaccu uccacacgau gcugaccccc ucccucaagg aggagauccg cgacaucguc     1080

aucuucuacc agcgcaagcu caagagccag aagaagcugg uguccaucug cgaguucgag     1140

aagcggaucc ucgagccccu gcagggggac aagguggucg gcuacaaggu ggcgcccaag     1200

agcagccccc uguuccagga guucaagauc uggcaggccc ucgggcgcau ggagauccag     1260

cacaagguga ccaaggagcg gcacuuccug gaccuggacg ccaagcagcu ccuguucgac     1320

gagcugaacg ucaagggcga ccucaccaag gagaacgccc ugacgauccu gggcuucaag     1380

cccaaggagu gggagugcaa ccuccugaag cuggagggga accgcaccaa ccaggcccug     1440

uacgaggcgu accucaagau ccuggagcug gagggcuacg aggugaagga ccuccugggg     1500

cugaagcucg acaaggacga caucaagcug uccgagcugc ccguggacgc cgccgacauc     1560

aagaagaugg uccgggagau cuucgagacc cucaacauca agaccagccu gcuggacuuc     1620

aacgccacgc ucccgggcaa guccuucgag cagcagcacg cguaccagcu guggcaccuc     1680

cuguacagcu acgaggggga caacuccccc agcggcaaca agaagcugca ggaccugcuc     1740

caggccaagu ucggcuucac caacgcccac gggcagaucc uggccaacgu gucccuggag     1800

accgacuacg gcagccucuc cgccaaggcg auccgcaaga ucuucaccca caucaaggag     1860

aaccacuaca gcgccgccug cgcccuggcc ggguacaacc acuccagcua ccuguccaag     1920

gaggagaacg agcagcggau ccucaaggac cgccugacca uccugcccaa gaacagccuc     1980

cggaaccccg uggucgagaa gauccugaac cagcugauca accuccugaa cacgaucuuc     2040

gacgacccgu accugggcaa gcccgacgag auccgcaucg agcucgcgcg ggagcugaag     2100

aagaacgugc aggagcgcca gcggcugaac accgagauca ccaagaacaa caccgagcac     2160

gagcgcauca cgaagauccu caucaaggag gacaacauca agaaccccac ccggaacgac     2220

aucauccgcu acaagcugua ccaggagcug aagcacaacg gguacaagac ccucuacucc     2280

aacaccuaca ucccccggga ggagcuguuc ggcaagaacu ucgacaucga gcacaucauc     2340

ccgcagagca uccuguucga cgacuccuuc agcaacaaga cgcucugcgu ccgcaaggag     2400

aacaucgaca aggccgaccg gaccgccaug gacuucaucg gccccuacuu cggggaggac     2460

aaggugcagg aguacaucaa ccgcguggag augcugcacc agaucaaccu gaagaacccc     2520

gaggagggca ucgggaaggg caaguacaag aagcuccuga ugaaggccuc cgagaucggc     2580

cagggguuca ucgagcgcga ccugcgggac acccaguaca ucgccaagaa ggcgcgccag     2640

augcuccugg aggucacccg gacgguggug gccaccaccg gcagcaucac caagcgccug     2700

cgggaggacu gggggcucgu caacgugaug caggagcuga acauggacaa guaccgccag     2760

cagggccuga cggagcucau cgagaagaag gacgggagca ccaaggagcg gaucaccgac     2820

uggaccaagc gcaacgacca ccggcaccac gccauggacg cccugacgau cgcguucacc     2880

aagcacuccc acauccagua ccugaacaac cucaacgccc gcaagagcga cgacaagaag     2940

ggcaaggaga ucuucggcau cgagcagaag gagaccgaga aggugaagga cgacaccggg     3000

cagacgcagc ggcgcuucaa gcugcccaug ccggacuucc ggcaggccgc caagcaccac     3060

cuggagcacc uccugaucuc cuucaaggcc aagaacaagg ucuucaccaa gaacaagaac     3120

aaguacaaga ccaagaacgg cgagcagguc aagcuggagc ucaccccgcg cgggcagcug     3180

cacaaggaga cgaucuacgg ccggaucaag aaguacguga cccacgagga gaaggugagc     3240

uccaaguuca acgcgcagcu gauccagacc guggccaaca gcaaguaccg cuacgcccuc     3300

cugaagcggc uggaggagaa cgggaacgac cccaagaagg ccuucaccgg caagaacucc     3360

cucgacaaga cgccccuguu cguggacgcc acccagcagg agaagauccc gcccgucgug     3420

aagcugguga gcaucgagca gggcuacacc auccgcaagg acaucacccc cgagaacuuc     3480

aaggacgcga aguccaucga gaagaucguc gacgugggga uccggaaccu ccugcaccac     3540

cgccuggaga cguacaacgg caacgagaag gaggccuucu acaaccucga caagaacccg     3600

aucuggguga acgagagcaa ggggaucugc aucaagcggg ucaccaucac cggcgugacc     3660

aacgccgagc cccugcacua caagcgcgac caccacggga agcccauccu ggacaacaac     3720

ggcaaccgca cccccgccga cuucgugucc acgggcaaca accaccacgc cgcgaucuac     3780

cgggacgccg ccgggaaccu ccaggacaac gucgugagcu ucuacgaggc cguggcgcgc     3840

gucaaccagg gccugccgau caucgacaag ucccugaaca aggagcucgg guggcaguuc     3900

cuguucagcc ugaagcagaa cgagugcuuc guguucccca acgagggcuu cgacccccuc     3960

gagaucgacc uguacaaccc cgccaacaac aagcugaucg ccgccaaccu cuuccgggug     4020

cagaacuucu cccgccugca guacgggaac aacaucaucc gggacuacaa cuuccgccac     4080

aucuacgaga ccagcgucga gaaccggaag gagcugaagg acaucgccua caagcagguc     4140

aagucccucc cgaucuucga gaagaucauc aaggugcgca ucaaccacau cggccagauc     4200

gugaagaccg gcgaguacug a                                               4221


<210>  2145
<211>  4224
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2145
augauccgca acauccuggg ccucgaccug ggggugagcu ccaucggcug ggccuacguc       60

caggaggaca gcaagaacuc cgagaacaac cggaucauca agcugggggu gcgcgugaac      120

ccccucaccg ucgacgagca gcugaacuuc gagaagggca agccgaucac cacgaacgcc      180

gggcggaccc uggcgcgcag cgcccggcgc aaccuccagc gguucaagcu gcgccggucc      240

aaccugaugg acgugcucaa gaagaacaac auccugaagg agaccgaccu gcucgccgag      300

gugggcaaga acagcaccuu ccagacgcag gagcugcgcg ccaaguccgc caaggagaag      360

aucgagcuga gcgaguucgc gcggguccuc cugcugauca acaagaagcg cggcuacaag      420

uccagccgga aggccaagaa cgacgaggac gggcagaucg uggacggcau ggccguggcc      480

aagaagcugu ucgaggagaa ccugaccccc ggggaguacu ccuaccagcu gcuccagcag      540

ggcaagaagc agcugcccga cuucuaccgc agcgaccugc aggcggaguu cgacaagauc      600

ugggacuucc agaagcaguu caaccccgag aucuucacca acgagcucua cgagaagcug      660

caggggaaga accggaacgc caccuggaag gagcuggagu ucccguucuc ccucgucggc      720

aucaagcaga cgggcaccau gcaggagaag aagguggaga aguacuucug gcgcagcgag      780

gccgugaaga agcagcugga cuucgagucc cuggccgucg uguuccagga gaucaacagc      840

aaccucaaca acuccagcgg guaccugggc gccaucuccg accggagcaa ggagcuguac      900

uucuacaacc agaccguggg ggaguaccuc uuccagcagc ugaagaccaa cccccacacg      960

aagcugaaga accaggucuu cuaccgccag gacuaccucg acgaguucga gaagaucugg     1020

gagacccaga gcaaguacca caaggagcug accaaggagc ugaaggagga gauccgggac     1080

aucaccaucu ucuaccagcg caagcucaag ucccagaagg gccugaucag caucugcgag     1140

uucgagaacc gggagaucga gaucacggag aacggcaaga ccaagaagaa gaccgugggg     1200

cugaaggugg cgcccaaguc cagcccccuc uuccaggagu ucaagaucug gcagguccug     1260

aacaaccugc aguuccagaa caucgagacc aaggaggugu ucccgaucga ccucgacuuc     1320

aagcagucca ucuucgacga ggugaacauc aagggcaagc ugagcgccaa ggacguccug     1380

gaccucgugg gguacuccgg caaggagugg aagacgaacu ucaaggagau cgaggggaac     1440

uacaccaacg agaaccugua caccgccuuc cugaagauca ucgccagcaa ggagaucgag     1500

uuccccaagg aguucaagau caccaucgac gacgagauca agguguccaa gaucaacgcc     1560

agcgcggaga ucaucaagcu guucguccgc gagaaguucu ccgagcucgg caucgacacg     1620

agcauccugg acuucaaccc cgagcuggag ggcuccgacu ucgagaagca ggccagcuac     1680

cagcucuggc accuccugua cuccuacgag ggggacgaca gcuccagcgg caacgagaag     1740

cucuacgagc ugcuggagaa caaguucggg uucaagaagg agaacuccaa gauccucgcc     1800

gagaucggcu ucagccagga cuacgggagc cuguccagca aggccaugcg caagaucuac     1860

cccuacauca aggagcacaa guacuccgug gccugcagcu acgcgggcua caaccacucc     1920

aagaacagcc ugaccaagga gcagcucgag aaccggaucc ugaaggagcg ccuggacauc     1980

cucccgaaga acucccugcg gaaccccguc guggagaaga uccugaacca gaugaucaac     2040

cucgugaacu gccugaucga cgaguacagc gagaaggaca aggacggcca gaucgucaag     2100

uacuucaagu ucgacgagau ccgcaucgag cuggcccggg agcucaagcu gaacgccgag     2160

gagcgcgcca ccaugaccuc cgagaucaac aaggccacga uccagcacca gaaguacgcg     2220

gaggugcugc agcgggaguu cgggcucaag gcccccagcc gcaacgacau cauccgguac     2280

aagcuguacc uggagcucgc caacaacggc uucaaggacc uguacaccga cgccaagauc     2340

gagcgcgaga accuguucac cgacaaguac gacaucgacc acaucauccc ccagucccgg     2400

uucuucgacg acagcuucuc caacaaggug cucgucccgc gcaacgcgaa ccugaagaag     2460

gggaacuuca ccgccuucga cuaccuggag aucgagggca aggagaagcu cgagaaguuc     2520

cugaacauca ucaaggaccu guacgacaag ggggccauca cgaaggccaa guacgagaag     2580

cuccagaaga aggacuucga gaucggcgac ggcuucaucg agcgggaccu gcgcaacacc     2640

caguacaucg ccaagaaguc caaggagauc cuguucggga ucaccaacag cguggugacc     2700

acgagcggcc ggaucaccga caagcuccgc gaggacugga accugaucaa caccaugaag     2760

gagcugaacc ucgagaagua ccggaagcug gggcugaccg agaccgucau caacuccaag     2820

ggcgaggaga agcagcgcau caucgacugg acgaagcgga acgaccaccg ccaccacgcg     2880

auggacgccc ucaccguggc cuucaccacc cacaaccaca uccaguaccu gaacuaccug     2940

aacgcccgca aggacgagaa gcacaaggag caccagaaca ucuucgccau cgagaaccuc     3000

aucacggaga ucaucgacaa gaagaacggc agcaaggagc ggcgcuucaa ggagcccgug     3060

aagaaccugc gggcggaggc ccgcaagcac cuggacgaga uccucaucag ccacaagguc     3120

aagaacaagg uggugaccaa gaacaucaac aagaucaaga agaagggguc cgucaucgcc     3180

aagaccgagc ugacgccccg gggccagcug cacaaggaga ccaucuacgg gagcuccaag     3240

uuccucaaga ccaaggagga gaagaucagc ggcaaguucg acguggagac cauccagaag     3300

gugcagaacg agcgcuaccg gaacgcccug cugaagcgcc uccaggaguu cgggggcgac     3360

uccaagaagg ccuucaccgg caagaacguc aucagcaaga accccaucuu ccugaacaac     3420

gagaagacgg agcagcugcc ggagaccgug acccucgcgu gguacgagau gggguacacc     3480

auccggaagg ccgugaaccc cgacaacuuc aaggacuuca agaacaucga gaagacgauc     3540

gacaagggca uccgcgacau ccugaccaag cggcugaagg aguucaacgg gaacuccaag     3600

gaggccuuca gcgaccucga caagaacccc aucuggcuga acaaguccaa gggcaucagc     3660

aucaaggccg ucaccaucac cgggaucaac aacgcggagg cccugcacua caagaaggac     3720

caccucggca aggacuuccu ggacgagaac ggcaagcgca ucgccgugga cuucgugucc     3780

acggggaaca accaccacgu cgccaucuac gaggacgaga acggcaaccu gcaggagaag     3840

guggugagcu ucuacgaggc cgucgagcgg gugaaccaga accuccccau caucgacaag     3900

gaguacaacu ccgagagcgg guggaaguuc cuguucacca ugaagcagaa cgagauguuc     3960

cuguucccgu ccgaggacuu caaccccaag gagauggacc ucuucgacga gaagaacgug     4020

aaccugauca gcaagaaccu guuccgcguc cagaaguuca ccaucaagga cuacuucuuc     4080

cggcaccacc ucgagaccaa cguggaggac uccagccccc ugaagggcau cacguggaag     4140

cgcgaggggc ugaacggcau cgagaacauc cucaaggugc ggcugaacca ccugggcaag     4200

aucguccaga ucggggagua cuga                                            4224


<210>  2146
<211>  4227
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2146
augaccaaga acauccuggg ccucgacgug ggggugagcu ccaucggcug ggcccugauc       60

aacgaggaca ucgagaaccc caagaacaac aagaucauca agcugggggu gcgcgugaac      120

ccgcucaccg ucgacgagca gaucaacuuc gagaagggca agcccaucac gaccaacgcc      180

gggcggaccc uggcgcgcgg cgcccggcgc aaccugcagc gguucaagcu ccgccgguuc      240

caccugaucg acauccugca gaagaacaac auccucaagc aggccgaccu gcuggccgag      300

gugggcaaga acagcaccuu ccagacgcag gagauccgcg ccaaguccgc gaagcagaag      360

guggagcuca gcgagcuggc ccggaucuuc cugcucauca acaagaagcg cggguacaag      420

uccagccgga aggccaagaa cgaggacgag ggccagauca ucgacgggau guucaucgcc      480

aagaagcugu acgacgagaa gcugaccccc ggcgaguacu ccuaccagcu ccugaaggag      540

agcaagaagc agcugcccga cuucuaccgc uccgaccucc agagcgaguu cgacaagauc      600

uggaacuucc agaagcaguu cuacccggag aacuucuccg acaaccugua caaggagcug      660

caggggaaga acaagaacgc gaccuggaag acccucgaga uccccuucag ccuggucggc      720

aucaagcaga cgggcacccu gcaggagaag aagcucgaga aguacuccug gcggagcgac      780

gccgugaaga agcagcugga cuacgagaag cuggccgugg ucuuccagga gaucaacucc      840

aacaucuaca acagcuccgg guaccucggc gccaucagcg accgcuccaa ggagcuguac      900

uucaacaacc agaccguggg ggaguaccug uacaaccaga ucaaggagaa cccccacacc      960

aagcucaaga accagguguu cuaccggcag gacuaccugg acgaguucga gaagaucugg     1020

gagacgcaga gcaaguacca caccacccug accaaggagc ucaaggagga gauccgcgac     1080

aucaucaucu ucuaccagcg gaagcucaag agccagaagg gccugaucuc caucugcgag     1140

uucgagaacc gcgagaucga gaucaaggag aacggcaaga ucaagaagaa gacggucggg     1200

cugaaggugg cccccaagag cuccccgcug uuccaggagu ucaagaucug gcagauccug     1260

aacaaccucc aguuccagaa caucgagagc aaggagaucu uccccaucga ccuggacuuc     1320

aaggagucca ucuucaacga ggugaacauc aagggccgga ucagcgcgaa ggccguccug     1380

gaccuccugg gguacuccgg caaggagugg aaccccaacu ucaaggacau cgaggggaac     1440

aacaccaacg agaaccugua caacgccuac cucaagauca ucgccgacga ggaguucgag     1500

cugcccaagg aguucaagcu gaacggcgac gacgaccugu acgugagcaa gaucgccugc     1560

uccgcgagca agaucaagga cuucgugaag gagaaguucu ccgagaucgg caucaacacc     1620

agcauccucg acuucaaccc ggagcuggag gggaaggacu ucgagaagca ggccuccuac     1680

caguucuggc accuccugua cagcuacgag ggcgacgacu cccagagcgg gaacgagaag     1740

cuguacgagc ugcucgagaa gaaguucggc uucaagaagg agcacuccaa gauccugagc     1800

aacaucgggu ucccccugga cuacggcaac cucuccacca aggccaugcg caagaucauc     1860

ccccaccuga aggccggccu gagcuucgcc aagaagucca aggaggacaa cauccucagc     1920

gcgacggcca uggccgggua caaccacagc aacuccuaca ccaaggagga ccuggccaag     1980

cggguccuga aggagaagcu cgagguguuc cccaagaaca gccugcgcaa cccggugguc     2040

gagaagaucc ugaaccagau gaucaaccuc gugaacgagg ccguggagga guacggcacc     2100

aucgacgagg uccgcaucga gcuggcgcgg gagcugaaga agaacgccga ggagcgcgcc     2160

aacaugaccu ccgagaucgg gaaggccacg cuccugcacc agaaguacgc ggagauccug     2220

aagaaggagu ucgacauccc cgugcccagc cggaacgaca ucauccgcua caagcucuac     2280

cuggagcugg ccaacaacgg cuacaaggac cucuacaccg acguccagau caagcgggag     2340

gagcuguuca ccaacaagua cgacaucgac cacaucaucc cccagucccg cuucuucgac     2400

gacagcuucu ccaacaaggu gcuggugccg cgcggggcca accucaagaa ggccaaccag     2460

accgccuucg acuaccugga gaacgaggac aagcagaagc uggagaaguu ccucaacauc     2520

aucaagaacc uguacgacaa gggcgagauc acgaaggcga aguacgagaa gcugcagaag     2580

aagggcagcg acaucgggga gggcuucauc gagcgggacc uccgggacac ccaguacauc     2640

gccaagaagg ccaaggagau ccuguucggg aucacccacu ccgucaucag caccuccggc     2700

aagaucacgg acaagcugcg cgaggacugg aaccucguga acaccaugaa ggagcugaac     2760

cuggacaagu acaagaagcu cggccugacc gagacccugc ucaacagcaa gggggaggag     2820

aaggagcgga ucaucgacug guccaagcgc aacgaccacc ggcaccacgc cauggacgcc     2880

cugacggugg cguucaccac ccacaaccac auccaguacc ugaacuaccu caacgcccgc     2940

aaggacgagg accacaagca gcacaagguc aucagcaaca ucgagaaccu gaucaccaag     3000

guguucgaga agaagaacgg cgccaagcag cggcgcuuca ucgagcccau caacaacuuc     3060

cggguggacg ccaagaagca ccuggacgag auccucaucu cccacaaggc caagaacaag     3120

gucgugacca agaacaucaa caagaucaag aagaagggga gcguggucgc gaagacggag     3180

cugacgcccc gcggccagcu gcacaaggag accaucuacg ggagcuccaa guuccucaag     3240

accaaggacg agaagaucag cggcaaguuc gaccuggaga ccaucaacaa ggugcagaac     3300

gagaaguaca agaacgcccu gcuccagcgg cugcgcgagu ucggcgagga cuccaagaag     3360

gccuucaccg ggaagaacgu gaucagcaag aaccccaucu accuggacga ggagaagacc     3420

cuccagaucc cggaguccgu cacccuggcc ugguacgaga ugaucuacac gauccgcaag     3480

cccgugaacc ccgacaacuu caaggacuac aagaacaucg acaaggugac cgacgagaag     3540

auccgggcgg uccugaccga ccgccuccgg aaguucaacg gcaacagcaa ggaggccuuc     3600

uccgaccugg agaagaaccc caucuggcug aacaagagca aggggaucgc caucaagacc     3660

gugacgauca ccggcauguc caacguggag gcccuccacu acaagaagga ccaccugggg     3720

aaggagaucc uggacaagaa gggcgaccgc augccggucg acuucgugag caccggcaac     3780

aaccaccacg ccgcgaucua cgaggacgcc aaggggaacc uccaggagcg gguggucucc     3840

uucuacgagg ccguggagcg cgugaaccag ggccugagcg ucgugaacaa ggacuacaac     3900

gccgggcugg gcuggaaguu ccucuucacc augaagcaga acgagauguu ccuguucccc     3960

uccgaggacu ucaaccccaa ggagaucgac cuguacgacg agaagaaccu caagcugauc     4020

gccaagaacc uguuccgggu gcagaaguuc ggggagcuca gcaaguccgg cuucugguuc     4080

cgccaccacc uggagacgag cgucgacgug aagaaggagc ugcggaacua cuccuaccuc     4140

gacuucuaca gcaaggacuu caugaagacc aucgugaagg uccgccugaa ccaccugggc     4200

aagaucgucc agguggggga guacuga                                         4227


<210>  2147
<211>  4230
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2147
augagcauca ucaacuucca gcgccggggc cugauggaga cccaggccuc caaccagcuc       60

aucagcuccc accugaaggg guaccccauc aaggacuacu ucgugggccu ggacaucggg      120

accagcuccg ucggcugggc cgugacgaac aaggcguacg agcuccugaa guuccgcagc      180

cacaagaugu gggggucccg gcuguucgac gagggcgaga gcgccguggc ccgccggggc      240

uuccgcucca ugcggcgccg gcucgagcgc cggaagcugc gccugaagcu ccuggaggag      300

cuguucgccg acgccauggc gcaggucgac ccgaccuucu ucaugcggcu ccgcgagagc      360

aaguaccacu acgaggacaa gaccaccggg cacuccagca agcacauccu guucaucgac      420

aagaacuaca cggaccagga cuacuacaag gccuacccca ccaucuacca ccuccgggcc      480

gagcugauga aggacggcac cgacgacauc cgcaagcugu uccuggccgu gcaccacauc      540

cucaaguacc gggggaacuu ccuguacgag ggcgcgaccu ucgacuccaa cgccagcacg      600

cuggacgacg ugaucaagca ggcccucgag aacaucaccu ucaacugcuu cgacugcaac      660

uccgccauca gcuccaucgg gcagauccug auggaggccg gcaagaccaa gagcgacaag      720

gcgaaggcca ucgagcaccu ggucgacacc uacaucgcca cggacaccgu ggacaccucc      780

agcaagaccc agaaggacca ggugaaggag gacaagaagc gccucaaggc cuucgccaac      840

cugguccugg gccucaacgc gagccugauc gaccuguucg gguccgugga ggagcucgag      900

gaggaccuga agaagcugca gaucacgggc gacaccuacg acgacaagcg ggacgagcuc      960

gccaaggccu ggagcgacga gaucuacauc aucgacgacu gcaaguccgu guacgacgcc     1020

aucauccugc ugagcaucaa ggagcccggg cucaccaucu ccgagagcaa ggucaaggcc     1080

uucaacaagc acaaggacga ccuggcgauc cugaaguccc uccucaagag cgaccgcucc     1140

aucuacaaca ccauguucaa gguggacgag aagggccugc acaacuacgu gcacuacauc     1200

aagcagggcc ggacggagga gaccagcugc aaccgcgagg acuucuacaa guacaccaag     1260

aaggucgugg aggggcugcc cgacuccaag gacaaggagu acauccugag cgagaucgag     1320

cugcagaccc uccugccgcu gcagcgcauc aaggacaacg gcgugauccc cuaccagcuc     1380

caccuggagg agcugaaggu cauccuggcc aagugcgggc ccaaguuccc cuuccucaac     1440

gagguggccg acggcuucuc cguggccgag aagcugauca agaugcugga guuccggauc     1500

ccguacuacg ucgggccccu caacacgcac cacaacgugg acaacggcgg cuucgccugg     1560

gcggugcgca aggccagcgg gcgggucacc cccuggaacu ucgacgacaa gaucgaccgc     1620

gagaaguccg ccgccgcguu caucaagaac cugaccaaca agugcaccua ccugcucggc     1680

gaggacgugc ugcccaagag cucccugcuc uacagcgagu ucaugcugcu gaacgagcuc     1740

aacaacgucc ggaucgacgg gaagccgcug gagaaggccg ugaaggagca ccugaucgag     1800

gccguguuca agcaggacca caagaagaug acgaagaacc gcaucgagca guuccucaag     1860

gacaacaacu acauccccga gaagcacaag cccgagauca ccggccugga cggggagauc     1920

aagaacgacc uggccuccua ccgggacaug guccgcaucc ucggcaacaa cuucgaccug     1980

agcauggccg aggagaucau caccgacauc accaucuucg gcgagagcaa gaagaugcug     2040

cgggagacgc uccgcaacaa guucuccagc cggcuggacg acgaggcgau caagaagcug     2100

uccaagcucc gcuaccggga cugggggcgc cugagccgga agcugcucaa cgugaucgag     2160

ggcugcgaca aggccgggga cggcaccccc gagaccauca ucauccugau gcgcaacuuc     2220

uccuacaacc ugauggagcu ccugggggac aaguucagcu ucauggagcg gauccaggag     2280

aucaacgcca agcugaucga gggccagauc gugaacccgc acgacaucau cgacgaccuc     2340

gcccuguccc ccgccgucaa gcgcgcggug uggcaggccc ugcggaucgu ggacgagauc     2400

guccacauca agaagacccu ccccgcccgc aucuucgugg aggugacgcg cagcaacaag     2460

gccgagaaga agaagaagga cucccggcag aagcgccuga gcgaccugua cgccgcgauc     2520

aagaaggacg acgcccuccu guccggccug accaacgagg aguucggggg ccucaagagc     2580

gggcuggcca aguacgacga cgccgcgcug cgguccaaga agcucuaccu guacuacacc     2640

cagaugggcc gcugcgccua caccggcgag gucaucgacc ugagccagcu caacaccgac     2700

aacuacgaca ucgaccacau cuacccccgg ucccugacga aggacgacag cuucgacaac     2760

cuggugcucu gcaagcgcac cgccaacgcc cagaaguccg acaccuaccc gaucagcaag     2820

gagauccaga aggcccagaa ggcguucugg aucuuccuga agcagcaggg gcugaucagc     2880

gagcggaagu acgagcgccu cacccggauc acgccccuga ccgccgacga ccuguccggc     2940

uucaucgccc gccagcucgu ggagaccaac cagagcguca aggccgccac cacgcugcug     3000

cggcgccucu accccggggu ggacgugguc uucgugaagg cggagaacgu gaccgacuuc     3060

cggcacgaca acgacuucau caagguccgc ucccugaacc accaccacca cgccaaggac     3120

gccuaccuga acaucguggu gggcaacguc uaccacgagc gguucacccg caacuuccgg     3180

gccuucuuca agaagaacgg ggccaaccgc accuacaacc ucgcgaagau guucaacuac     3240

gacgugaacu gcacgaacgc caaggacggc aaggccuggg acgugaagac cagcauggac     3300

accgucaaga agaugauggc cuccaacgac gugcggguga ccaagcgccu gcuggagcag     3360

agcggcgcgc ucgccgacgc cacggucuac aaggccgccg uggcggagaa ggccaaggac     3420

ggggccuaca ucggcaugaa gaccaagucc agcguguucg ccgacgucuc caaguacggg     3480

ggcaugacca agaucaagaa cgccuacagc aucaucgugc aguacgcggg gaagaagggc     3540

gaggugguca aggagaucgu gccccugccg aucuaccuga ccaaccggaa cacgaccgac     3600

caggaccuca ucgacuacgu ggccuccauc aucccccagg ccaaggacau cagcaucauc     3660

uaccgcaagc ugugcaucaa ccagcugguc aaggugaacg gcuucuacua cuaccucggg     3720

ggcaagacca acuccaaguu cugcaucgac aacgccaucc aggugaucgu cagcaacgag     3780

uggauccccu accugaaggu gcuggagaag uucaacaaca ugcgcaagga caacaaggac     3840

cucaaggcca acgugaucuc cacccgggcg cuggacaaca agcacacgau cgagguccgc     3900

aucguggagg agaagaacau cgaguucuuc gacuaccugg ugagcaagcu caagaugccc     3960

aucuaccaga agaugaaggg gaacaaggcc gccgagcugu ccgagaaggg cuacgggcug     4020

uucaagaaga ugagccucga ggagcagucc auccaccuga ucgagcugcu caaccugcug     4080

accaaccaga agaccaccuu cgaggucaag ccgcucggca ucacggccag ccggagcacc     4140

gugggcucca agaucagcaa ccaggacgag uucaagguga ucaacgaguc caucaccggg     4200

cuguacagca acgaggucac caucguguga                                      4230


<210>  2148
<211>  4233
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2148
augaccaaga acauccuggg ccucgaccug ggggugagcu ccaucggcug ggcccugguc       60

caggagaaca gcaagaaccc cgagaacaac aagaucguga agcucggggu gcgcgucaac      120

ccgcugaccg uggacgagca gaucaacuuc gagaagggca agcccaucac gaccaacgcc      180

ucccggaccc uggcgcgcag cgcccggcgc aaccuccagc gguucaagcu gcgccgggcc      240

aaccugaucg acgugcucaa gaaggggaac auccugaagc agggcgaccu gcucaccgag      300

aucggcaaga acuccacguu ccagacccag gagcugcgcg ccaagagcgc caaggagaag      360

gucgagcugu ccgacuucgu gcggauccuc cugcugauca acaagaagcg cggguacaag      420

agcucccgga aggcgaagaa cgaggacgag ggccagauca ucgacgggau ggccguggcc      480

aagaagcucu acgaggagaa caucaccccc ggcgaguaca gcuacaagcu gcugcaggag      540

gggcgcaagc agcuccccga cuucuaccgg uccgaccugc aggccgaguu cgacaagauc      600

uggaacuucc agaagcaguu caacccggag aucuucacca acgagcugua caagcgccuc      660

cagggcaaga accggaacgc gacguggaag gagcuggaga uccccuucua ccuggucggc      720

aucaagcaga ucgggaccau gcaggagaag aagaucgagc gcuacuucug gcggagcgag      780

gccgugaaga agcagcucga cuucgagucc cuggccgugg ucuuccagga gaucaacagc      840

aaccugaaca acuccagcgg cuaccucggg gccaucuccg accgcagcaa ggagcuguac      900

uucaacaacc agaccguggg cgaguaccug uacaaccagc ucaagaucaa cccccacacc      960

aagcugaaga accagguguu cuaccggcag gacuaccugg acgaguucga gaagaucugg     1020

gagacgcagg ccaaguacca caagaagcuc accaaggagc ugaaggagga gauccgcgac     1080

aucgucaucu ucuaccagcg gaagcugaag ucccagaagg gccucaucag cuucugcgag     1140

uucgagaacc gcgagaucga gaucaccgug aacgggaaga ccaagaagaa gacggugggc     1200

cugaaggucg ugccccgcag cuccccgcug uuccaggagu ucaagaucug gcagcggcuc     1260

aacgaccugg aguucaagaa cguggagacc aaggagaucu ucccccugga gcucgagcac     1320

aaggagaucc uguucgggaa ccugaacauc aagggcaacc ucagcuccaa ggagcugauc     1380

gagcagcugg gguaccccag caaggagugg aagugcaacu ucaaggagau cgacggcaac     1440

aagacccacg agaaccugua caccgcguuc cucaagauca ucgccaacga gggcaucgag     1500

uuccccaagg aguucaagcu guccaucgac gacgacauca aggucagcaa gaucgacgug     1560

uccagcgaga agaucaagga guucaucaag gagaacuucc ugaagcucgg gaucaacacg     1620

uccauccugg acuucaaccc ggagcuggac ggcaaggacu ucgagaagca ggagagcuac     1680

cagcucuggc accuccugua cuccuacgag ggggacgaca gcgccuccgg caacgagaag     1740

cuguacgagc ugcuggagaa gaaguucggg uucaagcgcg aguacagcaa gauccucgcc     1800

gacgugggcu ucucccagga cuacggcagc cuguccagca aggccaugcg gaagaucuuc     1860

accuacauca aggagaacaa guacuccgag gcgugccacc uggccgggua caaccacagc     1920

aagaacagcc ucaccaagga gcagcuggcc aaccgcaucc ugaaggagca gcucgagauc     1980

cugcccaaga acgcccugcg gaaccccguc guggagaaga uccucaacca gaugaucaac     2040

cuggucaacg aggugaucaa ggaguacggc aagcccgacg agauccgcau cgagcuggcc     2100

cgggagcuca agaagaacgc ggaggagcgc gccaacauga ccuccgacau cagcaaggcc     2160

acgcugcugc accagaagua cgccgaggug cuccagaagg aguucgggau caaggccccg     2220

ucccggaacg acaucauccg cuacaagcug uaccuggagc ucgcgaacaa cggcuacaag     2280

gaccuguaca ccaaccagaa gaucgagaag gagaacaucu ucaccgacaa guacgacauc     2340

gaccacauca ucccccagag ccgguucuuc gacgacuccu ucagcaacaa gguccuggug     2400

ccccgcugcg ccaaccagga caaggacaac uacaccgccg uggacuacau cgagaagaag     2460

uacggggagc ggggcaagga ggacuuccuc aaccgcaucc accuggucuu caaguccaag     2520

gaggccaucg cgggcaagag cgagucccag aucagcucca ugaacacgaa guuccugaag     2580

cuccagaaga aggggagcga gaucggcgag ggguucaucg agcgggaccu gcgcgacacc     2640

caguacaucg ccaagaaggc caaggagauc cuguucggca ucaccaacuc cgugaucagc     2700

accuccggcc ggaucacgga caagcuccgc gaggacugga accuggugaa caccaugaag     2760

gagcugaacc ucgagaagua ccggaagcug gggcugaccg agcucaucgc caacagcaag     2820

ggcgaggaga aggagcgcau caucgacugg accaagcgca acgaccaccg gcaccacgcc     2880

auggacgcgc ugacggucgc cuucaccacc cacaaccaca uccaguaccu gaacuaccuc     2940

aacgcccgca aggacgagaa gcacaaggag caccacaaca ucuucgccau cgagaacauc     3000

aucaccgaga ucguggagaa gaagaacggg uccaaggagc ggcgcuucaa gcugcccauc     3060

aagaacuucc ggaccgaggc caagaagcag cuggaggagg ugcucaucag ccacaaggcg     3120

aagaacaagg ucgugacgaa gaacaucaac aagaccaaga agaagggcgg gguggucagc     3180

aagaucgugc ugaccccgcg cggccagcug cacaaggaga ccaucuacgg cuccgccaag     3240

uuccucaaga cgaaggagga gaagaucagc gggaaguucg accuggagac caucaacaag     3300

gugcagaacg agaaguucaa gaacgcccug cucgaccggc ugaaggaguu caacggcgac     3360

uccaagaagg ccuucaccgg gaagaacguc cccgccaaga accccaucua ccugaacgag     3420

cagaagaccg agcagguccc cgagagcgug gugcucgcgu gguacgagac gggcuacacc     3480

auccgcaagg ccgugaacgc cgacaacuuc aaggacuaca agaaccugga gaagaucauc     3540

gacaaggggg ugaaggagau ccugaagaac cggcucaacg ccuucaacgg caacgcgaag     3600

gaggccuucu ccgaccugga gaagaagccg aucuggcuga accagagcaa gggcaucgcc     3660

gucaagaccg ugaccaucac ggggaucaac aacgccgaga cccuccacua caagaaggac     3720

cacuucggca acgagcugcu ggacgagaac gggaagaaga uccccgugga cuucgucucc     3780

accggcaaca accaccacgu ggccaucuac gaggacgcgg acgggaaccu ccaggagcgc     3840

guggucagcu ucuacgaggc cguggagcgg gugaaccagg gccuguccau caucgacaag     3900

gaguacaacc aggaccuggg cuggaaguuc cucuucacca ugaagcagaa cgagauguuc     3960

cuguucccca gcgaggacuu caaccccaag gagacggacc uguucgacga gaagaaccuc     4020

aaccugaucu ccaagaacau guuccgcguc cagaaguucg ggagccugcu cuccggcuuc     4080

ugguuccggc accaccugga gaccaccgug gaccugaaca gcucccucaa ggacaccagc     4140

uacaagguga uccaguccug caacaaccug aagcugaucg ucaaggugcg ccucaaccac     4200

cuggggaaga ucgugcagau cggcgaguac uga                                  4233


<210>  2149
<211>  4236
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2149
auggugaaga aggaguacuu caucggccug gacaugggga ccagcuccgu cggcugggcc       60

guggccgaca ccaacuacaa ccuccugcgc gcgaagggga aggacaugug gggcgugcgg      120

cuguuccccg aggccagcac ggccgccgac cgccgguccc accgcaccgc ccggcgcaac      180

aacaagcgga aggcggcccg cgagggguuc cuccgggaga ucuucgagga cgagaucaac      240

aagaucgacc cgggcuucuu cgagcgccug aaggagagca aguacuaccu ggaggacaag      300

cucaccgaca cccccuucgu ccuguucgcc gacaagggcu acacggacaa ggaguacuac      360

gacgaguacc ccaccguguu ccaccugauc aaggagcuca ucuccgagaa ggggcuggag      420

ccccacgaca uccggcuggu guaccucgcc ugccugaaca ucuacaagca ccgcggccac      480

uuccugaacg acacccucag cggggacggc aucgacaaca ucaacucccu gugcaacgac      540

uucaccagcu accuggcgga cuacaaccag aacuccgagg acgacgggac ggagaagaug      600

gaccucgaca gcaccguccu ggccggcgug uucgaggaga cccugugcaa caagcaccuc      660

uccaacagcg ccaagaagcg ggaccugauc gacaagcugg gcaucaccaa caagacgaag      720

uaccucaccg agaugauggc ccugaucucc gggcuggcca gcuccgugag caacauguuc      780

gcgaaggaca ccuacgacga ggagcagaag aaguucaagc ucuccuuccg cagcuccaac      840

cuggacgagg acauggucaa ggugcaggcc augcugaccc cggaggagua cgagaugauc      900

ugcaucauga agaagaucca cgacuggagc guggucagcg ugaucauggg cucccacaag      960

uacaucagcg acgcccgggu ggagcucuac gagaagcaca agcaggaccu ggccaagcug     1020

aaggccaucu acaaggagua ccuccccgac aaguacgacg cgauguuccg cgagaugacg     1080

gacaacuccu acagcgccua cgucgggaag guguccagca acaagaaguc ccagaccggc     1140

aaccacaagg ugcggaugca gcgccggggc gccaagugca agcaggagga cuucuacgac     1200

cgcaucaaga aggagaucaa cagcgucccc caggaggaga agaucgacau ccugcaggac     1260

auggagacca acuccuuccu gcccaagcag cucaccaacg agaacggggu gaucccguac     1320

cagcugcacc uggccgagcu caagaagauc cuggacaacg ccagcacgua ccugcccuuc     1380

cucaacuccg cggacgagac cggcaucagc aacaaggaca agaucaucca gcuguucgag     1440

uuccggaucc ccuacuacgu ggggccccug uucaaguccg agaagaacaa gaccaacgcc     1500

ugggucgugc gcaaggagag cggcaaggug uacccgugga acuucaacga gaagaucgac     1560

gagaagcagu ccgccgagau cuucaucgag cgcauggucc ggcacugcac cuaccucaac     1620

aacgagagcg cccugcccaa gaacucccug aaguacgagc gcuuccgggu gcucaacgag     1680

cugaacaacc ugcgcaucaa cggggagcgg aucagccccg agcugaagca gcgcaucuac     1740

aacgagcugu ucaaggucaa gaagaagcgg aucacgaagu ccaugcucug cgaguaccug     1800

uacaagaacg gcaucgccga caaggacagc gagcucagcg gcuucgacgg gaccuucgcg     1860

aacgcccugc uguccuacgg caaguuccgc gagcuguuca acguggacac ccucaccgac     1920

aagcaggagg ccgccgcgga ggacaucauc aaguggagca cgguguacgg ggacuccaag     1980

aaguuccugc aggagaagau cgaggagaac uacggccccg agagcgagaa cccgcuccug     2040

gacaacaagc agaucaagcg ggucaugggg uacaaguuca ccgacugggg caaccugucc     2100

cgcgaguucc ucaaccugga gggcgccgac aaggacaacg acaccaccac gggggaggug     2160

augccccuga ucacccggau gugggaggag aacuacaacc ucauggagcu gaugagcaac     2220

aaguucuccu accgcgacgc ccuggccgag aagaccgagg agaucgagaa gacccucacg     2280

gacaucgagu acgaggaccu ggaggaccug uacaucagcg cccccgugaa gcggaugacc     2340

uggcagaccc uccugauccu gaaggagauc uaccagauca ugggcuacgc gcccaagaag     2400

aucuucgucg agauggcccg cgaggaggag gagaagguga acgggaaggg cgcccggaag     2460

uccagccgca agaagcggcu cgaggaccug uaccgcaacu gcaaggacgc cuccaaggag     2520

cugagcaccg ggaucaagaa cgccgacgag gcgauccucc ggcgcaagaa gcuguaccug     2580

uacuaccucc agaagggcaa gugcauguac uccggcaaga ccaucgacuu caagagccug     2640

uucacgaaca acuacgacau cgaccacauc uacccgcagu ccguggucaa ggacgacagc     2700

cuggagaaca acauggugcu cguggagcgc aacuacaaca acaagaaguc cgacaccuuc     2760

cccaucuuca gcgacuggca gaacaagaug aagcccuucu ggaccaugcu gaaggagcag     2820

ggguucauca acgaggagaa guacaagcgg cugacccgca cggacgagcu caccgacgac     2880

gagcuggcca acuucgucaa ccggcagcug guggagaccc gccaggccac caaggugauc     2940

gccgagcucc ugaagaacag cuucgacgac aucgacucca agaucgucua cgccaaggcg     3000

ggcaacguga gcgaguuccg gcacaaguuc uccaugaagu acgacaagga gacggagaag     3060

uccagcguga uccaccccga gcuggucaag ugccgcauca ucaacgacuu ccaccacgcc     3120

aacgacagcu accucaacau cguggugggg aacaucuacg acgucaaguu cacccagaac     3180

ccguacaacu acaucaagga guacaagaag cagaagaaca acuccgcccu gagcgagcag     3240

gagaaggaga aguaccacau ggacaagauc uucaacuucg acgugaagcg gaacggcgag     3300

guggccugga ucucccgcgg cgacaacaag acccuggaga ccguccucaa ggugaugcgg     3360

aagaacacgc ccaucgugac ccgcaugaac uucgaggagc acgggcagcu ggcggaccug     3420

accaucuaca gcgccuccga cgcccucagc aagaagggcg ucggguacau caaggugaag     3480

gccuccgacg cccggcugga cgugcugaag uacggcgggu acaagaagua caagggcgcg     3540

uacuucuucc ucgucgagca caccgagaag aacaagcgcg ugcggacgau cgagaacaug     3600

ccccuguacc ugaagaacga gcucaacacc aaggagaaga ucgagcugug gugcgccgac     3660

aaggagaacg gccuggggcu cguggacccc gacguccgcc ugggcaagau caagcuguac     3720

agcaagaucc ggaucgacgg guucgaccuc uaccugaccg gccgcaccgg gaacaaccug     3780

cucacgucca acgccgugca gcugaagauc ggcagcaaca agccguccga caacgacaag     3840

cuguucuggc gguacuacau caacaagcuc agcaaguaca ccgagaacuc cgaguucgug     3900

agcauccaga acaacaucaa gcuguacgac cuguucgucg agaucaacaa cgccggcgug     3960

uacagcaagc gccagaaccc caucggggac aagcuccugg agggccgguc caaguucauc     4020

aaccugaccc ucagcaagca ggucgagacc cugcugggga ucauccgcau cuacucccuc     4080

gagaaccagg gcgccgaccu gagcaacauc gggggccagg agaagaacgg cacgaugaag     4140

ccccacaagc ggaucuccga caagaaggag gugcugcuca ucaaccagag cgugaccggg     4200

cuguacgagu ccaagaucga ccugcucaag aucuga                               4236


<210>  2150
<211>  4236
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2150
augaagaaca uccugggccu cgaccugggg acgaacagca ucggcugggc ccugaucaag       60

cagaacgccg aggagcgcca gggguccauc cucggcaugg ggacccgggu gcugcccaug      120

agccaggacg uccugggcga cuucggcaag gggaacuccg ugagccagac ggcggcccgc      180

accggcuacc gggggauccg ccggcuccgc gagcgguucc ugcugcgccg ggagcgccuc      240

caccgggugc ugaacauccu gggcuuccuc ccguacuacu acgccgccca gaucgacuuc      300

gacaagcgcu ucgggaaguu caaggccgag accgagccca agcugcccua caacaacgag      360

ggcuucaucu ucaagaacuc cuucgaggag augcugagcg acuucaagau cucccagccc      420

gagcuccuga gcggcaacaa gaaggucccg uacgacugga ccaucuacua ccugcggaag      480

aaggcggugu cccagaagau cgagaaggag gagcucgccu ggauccugcu gaacuucaac      540

cagaagcgcg gguacuacca gcuccggggc gaggacuucg acgaggagaa ggacaagacg      600

uucgugaccc ugaacgucga caaggugauc gacagcgggg agaaggugaa gggcaacauc      660

cuguucgaca ucuacuucac caacggcugg aaguacgaga agcagaccac gaagcccgag      720

gacuggcucg ggcgcuccaa ggaguucauc aucaccgaga gccugcugaa gaacggcgag      780

guccggcgca ccuucaaggc cguggacucc gagaaggacu ggaucgccau caaggcgaag      840

accgagcagg agaucgagac ggcccacaag accgugggga gcuacaucua cgacgccauc      900

cuccagaacc ccaagcagaa gaucaagggc aagcuggucc ggaccaucga gcgcaaguuc      960

uacaaggagg agcugcggaa gauccucgag aagcagaagg acuuccaccu ggagcugcag     1020

aacgaggacc ucuacuccga cugcgugcgc gagcuguacc gcaacaacga cacccaccag     1080

cugagccucg ccaagaaguc cuucauccac cuguuccugg aggacaucau cuucuaccag     1140

cggccccuca agagccagaa guccagcgug agcaacugca cgcuggagug ccgcaaguac     1200

cgggacaaga acggggccga gggcauccag uuccugaagg ucaucccgaa guccaacccc     1260

uacuaccagg aguuccgcau cuggcagugg augagcaacc ucaagaucua ccggcgcgac     1320

gacgacguga acaucaccuc cgagugccug aagaccgcgg aggacuacga ggaccuguuc     1380

guguuccuca acauccggaa ggaggucgac cagaaggccu gccugcgcca cuucaagcug     1440

agcgagaaga cccaccggug gaacuacgug gaggacaaga aguaccccug caacgagacg     1500

ggcaccauga ucuccgagcg ccucaagaag gugggggaca gccccgacaa caucuucacc     1560

cgggagaugg agcagcagau cuggcacauc aucuacuccg ucaacgacaa gaccgaguac     1620

gagaaggccc ucaagagcuu cgccaagaag aacgagcugg acaacgacuc cuucuucgag     1680

gccuuccgca aguucccgcc cuucgacagc gaguacggcu ccuucagcga gaaggcgauc     1740

aagaagcugc ucccccugau gcggaucggg aagcacugga acugggaggc cguggacgag     1800

aagaucaaga accgcaucca gaagaucauc aucggcgagu acgacgagaa caucaaggac     1860

aacauccggg agaagacgcu gucccuccag aaggagagcg acuuccaggg gcugccccug     1920

uggcucgccc aguacguggu cuacggccgc cacuccgagg ccagcguggc cggcaagugg     1980

aacuccgucg acgacaucga ggaguaccug aaggaguuca agcagcacag ccugcggaac     2040

ccgaucgugg agcaggucau cauggagacc gugcgcgugg ugaaggacau cuggaucaag     2100

uacgggaacg gcgcggccga cuucuucgac gagauccaca ucgagcucgg gcgggagaug     2160

aagcugcccg ccaaggagcg caacaagaug acccagaaga ucuccgagaa cgagaacacc     2220

aaccugcgca ucaaggcccu ccuggccgag augaugaacg acagcgaggu ccagaacgug     2280

cggcccuaca gccccaugca gcaggagauc cugaagaucu acgaggacgg cgcgcucaac     2340

uccgagauca gcguggacga cgacauccug aagaucucca agacggccca gccgagcccc     2400

uccgaccuga agaaguacaa gcucuggcug gagcagaagu accgcagccc cuacaccggg     2460

gacaucaucc cccugaacaa gcuguucacc accgaguacg agaucgagca caucaucccg     2520

cagucccggu acuucgacga cagcuucucc aacaagguca ucugcgagag ccuggugaac     2580

cgccugaagg acaacuacau cggccucggc uucaucaagc aguuccacgg guacaagauc     2640

uccgacaaga cgaagguguu cgaggucgag gaguacgagg acuucgugaa gaagaacuac     2700

gccaacaacc ggagcaagcg cuccaagcug cugcucgagg agauccccga ggagaugauc     2760

gagcggcagc ugaacgacac ccgcuacauc agcaaguaca ucuccggcau ccugagcaac     2820

aucgugcgga agaaggagaa cgacgagggg gucaacucca aggacaucau ccccggcaac     2880

ggcaagauca ccagccagcu caagcaggac ugggggcuga acgacgugug gaacgaccug     2940

auccuccccc gcuucgagcg gaugaacgag cugaccaaga gcuccuacuu cacggugugg     3000

aacgagaagu accagaaguu ccugccgacc guccccgugg aguucagcaa gggcuucucc     3060

aagaagcgca ucgaccaccg gcaccacgcc cucgacgcgc uggugaucgc cugcgccacc     3120

aaggaccacg ucaaccugcu caacaacaag agcgccaagu ccgagaugaa gcgcuacgac     3180

cugaagcgga agcugaugaa guucgagaag aagaccuacc agcacagcca gaccggggag     3240

cgcaucguga aggacgugcc cgucgaguuc cucaagccgu ggaacgacuu cacgguggag     3300

gccaaggaga accuggagaa gaucaucgug uccuucaagc agaaccugcg ggucaucaac     3360

aaggcgacca accacuacga gaaguggauc gagaaggaca acgugaaggu gaagggccuc     3420

gucgagcagc aggggcugaa cugggccauc cgcaagcccc ugcacaagga caccgugagc     3480

ggcaaggugg accuccccgg caucaagguc cccaagggga agauccugac cgccacgcgg     3540

aaguccaucg acaccagcuu cgaccugaag accaucaacu ccaucaccga cacgggcgug     3600

cagaagaucc ucaagaacua ccuggaguac aaggggaaca gcuccgagga ggccuucagc     3660

uccgagggca ucgaggacau gaacaacaac aucgagaagu ucaacgaggg gaagcugcac     3720

cagccgauca ccaaggugcg caucuucgag cucggcagca aguucccccu gggccagauc     3780

gggaacaaga agcacaagua cgucgagacc gccaagggca ccaaccuguu cuucgcgauc     3840

uacgaggacg acaaggggaa gcggacguac gagaccaucc cccucaacga ggugaucgag     3900

cgccagaagc agggccuggc ccccgugacc cugaagucca agaacgacuu cuaccucugc     3960

ccgaacgacc uggucuacgu ggccgccgag ggggagagcg ugaacaagau cgacuucaag     4020

uacgccuccc aggaguucau ccacaacauc uacaaggucg ugagcuucag cggcucccag     4080

aucuucuucg ugaagcagaa caucgcgguc agcaucguga acaaggccga guucuccacc     4140

cugaacaaga uggagcgcgc cguggacggc aagaugauca aggacugcug caucaagcuc     4200

aagaucgacc ggaucgggaa caucaccaag agcuga                               4236


<210>  2151
<211>  4239
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2151
augaugacca agaacauccu gggccucgac cuggggacca acagcaucgg cugggcccug       60

aucaagcaga acgccgagga gcgcaagggg uccauccucg gcauggggac gcgggugcug      120

cccaugagcc aggacgcgcu gggcgacuuc ggcaagggga acuccgucag ccagaccgcc      180

gcccgcaccg gcuaccgcgg ggugcggcgg cuccgcgagc gguuccugcu gcgccgggag      240

cgccuccacc gggugcugaa cauccugggc uuccucccgg acuacuacgc cgcccagauc      300

gacuucgaca agcgcuucgg gaaguucaag gcggagaccg agcccaagcu gcccuacaac      360

aacgagggcu ucaucuucaa gaacuccuuc gaggagaugc ugagcgacuu caagaucucc      420

cagcccgagc uccugagcga gaacaagaag aucccguacg acuggacgau cuacuaccug      480

cggaagaagg ccgucuccca gcgcaucgag aaggaggagc ucguguggau ccugcugaac      540

uucaaccaga agcggggcua cuaccagcuc cgcggggagg acuucgacga ggagaaggac      600

aagaccuucg ugacccugaa cgucgacaag gugaucgaca gcggcgagaa ggugaagggg      660

aucguccugu ucgacgugua cuucaccaac ggcuggaagu acgagaagca gaucacgaag      720

cccgaggacu ggcucggccg guccaaggag uucaucauca ccgagagccu gcugaagaac      780

ggggagguga agcgcaccuu caaggccguc gacuccgaga aggacuggau cgccaucaag      840

gcgaagaccg agcaggagau cgagaucgcc cacaagacgg ugggcagcua caucuacgac      900

gccauccucc agaaccccaa gcagaagauc aaggggaagc ugauccggac caucgagcgc      960

aaguucuaca aggaggagcu gcggaagauc cucgagaagc agaaggaguu ccaccccgag     1020

cugcagaacg aggaccugua cuccgacugc gugcgcgagc ucuaccgcaa caacgacgcc     1080

caccagcuga gccuggccaa gaaggacuuc auccaccucu ucgucgagga caucaucuuc     1140

uaccagcggc cgcugaaguc ccagaagucc agcgugagca acugcacccu ggagugccgc     1200

aaguacaagg acaagaacgg cgaggagcgg auccaguucc ucaaggugau ccccaagagc     1260

aaccccuacu accaggaguu ccgcaucugg caguggaugu ccaaccugaa gaucuaccgg     1320

cgcgacgacg acgucaacau caccagcgag uaccugaaga cgaccgagga cuaccaggac     1380

cucuucgugu uccugaacac ccggaaggag guggaccaga aggcgugccu gaagcacuuc     1440

aagcucaacg agaagaccca ccgcuggaac uacgucgagg acaagcggua ccccugcaac     1500

gagacgggga ccaugauccu ggagcgcuuc aagaaggugg gcgacuccgc cgacaacauc     1560

uucacccggg agauggagca gcagaugugg cacaucaucu acagcgugaa cgacaagacc     1620

gaguacgaga aggcccugaa guccuucgcc aagaagaacg accucgacaa cgucagcuuc     1680

uucgaggccu uccgcaaguu cccgcccuuc gacuccgagu acggcagcuu cuccgagaag     1740

gcgaucaaga agcugcugcc ccugauccgg acggggaagu acuggaacug ggaggccgug     1800

gacgagaaga ccaagaagcg cauccagaag augaucaccg gcgaguggga cgaggacauc     1860

aaggacaaca uccgggagaa gacccucagc cugcagaagg aguccgacuu ccaggggcug     1920

ccccucuggc uggcccagua cgccgucuac ggccgccaca gcgaggccuc cguggcgggg     1980

aaguggaaca gcgugaacga cauggaggag uaccugaagg aguucaagca gcacucccuc     2040

cggaacccga ucgucgagca ggucaucacg gagaccaucc gcguggugaa ggacaucugg     2100

aucaaguacg gcaacggcgc cgccgacuuc uucaacgaga uccacaucga gcuggggcgg     2160

gagaugaagc ugcccgccaa ggagcgcagc aagaugaccc agaagaucuc cgagaacgag     2220

aacaccaacc uccgcaucaa ggcccugcug guggagauga ugaacgacag cgaggugcac     2280

aacguccggc ccuacucccc caugcagcag gagauccuca agaucuacga ggacggcgcg     2340

cugaacagcg agaucagcgu ggacgacgac auccugaaga ucuccaagac ggcccagccg     2400

agccccuccg accucaagaa guacaagcug uggcuggagc agaaguaccg cagccccuac     2460

accggggaca ucaucccccu caacaagcug uucaccaccg aguacgagau cgagcacauc     2520

aucccgcagu cccgguacuu cgacgacagc uucuccaaca aggugaucug cgagagcucc     2580

gucaaccgcc ugaaggacaa cuacaucggc cucggguuca ucaagcaguu ccacggcuac     2640

aagaucagcg acaagacgaa gguguucgag guggaggagu acgaggacuu cgucaagaag     2700

aacuacgccu ccaacuggag caagcggucc aagcugcugc ucgaggagau ccccgaggag     2760

augaucgagc gccagcugaa cgacacccgg uacaucagca aguacaucuc cggcauccug     2820

agcaacaucg ugcgcaagaa ggagaacgac gacgggguga acuccaagga caucaucccc     2880

ggcaacggga agaucaccag ccagcucaag caggacuggg gccugaacga cgucuggaac     2940

gaccuggugc ucccccgguu cgagcgcaug aacgagcuga ccaagagcaa cgacuucacc     3000

guguggaacg agaaguacca gaaguuccug ccgacggucc ccguggaguu cuccaagggc     3060

uucagcaaga agcggaucga ccaccgccac cacgcccucg acgcgcuggu gaucgccugc     3120

gccacccggg accacgucaa ccugcucaac aaccaguccg cccgcagcga gaugaagcgg     3180

uacgaccuga agcgcaagcu gaugaaguuc gagaagaagg ccuaccagca cucccagacc     3240

ggggagcgga ucgugaagga cgugcccguc gaguuccuca agccguggga caacuucauc     3300

guggaggcga aggagaaccu ggaccgcauc aucgugagcu ucaagcagaa ccugcggguc     3360

aucaacaagg ccaccaacca cuacgagaag uggaucgaga agaacaacgu gaaggugaag     3420

gcccucgucg agcagcaggg ccugaacugg gccauccgca agccccugca caaggacacg     3480

guguccggga agguggaccu ccccggcacc aaggucccca aggggaagau ccugaccgcc     3540

acccgcaaga gcaucgacac guccuucgac augaagacca ucaacagcau caccgacacc     3600

ggcauccaga agauccugaa gaacuaccuc gaguacaagg gcuccuacga ggacgcguuc     3660

agcccggagg ggaucgagga caugaacaag aacaucgaga aguucaacga cggcaagcag     3720

caccagccca ucaucaaggu gcggaucuuc gagcuggggu ccaaguuccc ccugggccag     3780

aucgggaaca agaagcacaa guacguggag acggccaagg gcaccaaccu cuucuucgcc     3840

aucuacgagg acgacaaggg caagcgcacc uacgagacca ucccccugaa cgaggucauc     3900

gagcggcaga agcaggggcu ggccccggug acguucaaga gcaagaacga cuucuaccuc     3960

ucccccaacg accuggugua cgucgccgcg gagggcgaga gcguggacaa ccuggacuuc     4020

caguccgaca gccaggagua caucaacaac guguacaagg ucguguccuu cagcgggagc     4080

cagaucuucu ucgugaagca gaacgucgcc guguccaucg ugaacaaggc cgaguucagc     4140

acccucaaca agauggagcg cgccaucgac ggccggauga ucaaggagug cugcgucaag     4200

cugaagaucg accgcaucgg gaacaugacc aaguccuga                            4239


<210>  2152
<211>  4245
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2152
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cuucguggag       60

gaggccgaga acgaguccga gaaguccucc aucaucaagc uggggguccg cgugaacccc      120

cucaccgugg acgagcagca gaacuucgag aagggcaaga gcaucacgac caacgcggac      180

cggacccuga agcgcuccau gcggcgcaac cugcagcggu acaagcagcg ccgggagaac      240

cucaucgaca uccugaugaa gaacggguuc accagcgacg acacgauccu guccgagaac      300

ggcaaccgca ccaccuucga gaccuaccgg cuccgcagca aggccgucgc cgagaagauc      360

ucccugcagg aguucgcccg ggugcugcuc augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaaggggac ggaggacggc gagcugaucg acgggaugga gguggcgaag      480

aagcuguaca acuacaaccu caccccgggc cagcugugcc ugcagcuccu gaacgagggg      540

aagaagcacc uccccgacuu cuaccgcagc gaccugcagg ccgaguucga ccggaucuuc      600

gacauccaga ugaccuucua caccgaguac cugacgcugg agcucaagga ggagcugcgc      660

ggcaagaagc gggacgccgu cugggccauc ugcgcgaaga ucuucaagga gaagggcuuc      720

gagcugaucg ggaccaagcg ccagggcucc accgccgagc agaagcacga gaacuacagc      780

uggcgggcca aggggcucuc cgagaagcug gagcuggagc agcucguggu gguccugcag      840

cagaucaaca uccagcugaa cagcuccagc ggcuaccucg gcgccaucuc cgaccgcagc      900

aaggagcugu ucuucaacaa gcagaccgug gggcaguacc agauggccgu gcuggacaag      960

aaccccaacg cgggccuccg gaacaugguc uucuaccgcc aggacuaccu ggacgaguuc     1020

aacgccaucu gggagaagca ggcccuguuc caccccaucc ucacggacac ccugaaggac     1080

gagauccgcg acgugaucau cuucuaccag cggcgccuca agagccagaa ggggcugauc     1140

uccuucugcg agcuggagag ccggaagauc gagguggaga ucgccggcca gaacaagacc     1200

aagauggucg ggucccgcgu gaucccgcgg agcagccccc uguuccagga guucaagauc     1260

uggcagagcc ucaacaacau cgagguguuc ggcaagggca agaaguccaa gaagcagaag     1320

gagcagaccc uguucgacca gacggaggac ccccuggagu ucgggggccg ccgggagcuc     1380

uacgaggagg agaaggagcu gcuggcccug gagcucagca ucaagaagga gcugaagaag     1440

gcggacaucc ugaagcuccu guucgacaac cccaaggagc uggaccucaa cuucaacgcc     1500

guccagggga accugaccca ggccacccug uucaacgugu acaagaagau caucgagcuc     1560

uccggccacg agaucgacgu ggagaaggac agcgccucca agauccucaa gagcaucgag     1620

gagaucuuca aggcccugaa cuacaacacc gagauccugc acuucgacuc cgagaagucc     1680

caggaccagc aggcgaugua caugcugugg caccuccugu acagcuucga gggggacaac     1740

agcaacacgg gcaacgagaa ccugaucaac aagaucucca agcuguacgg cuucgagaag     1800

gaguacgcca agauccucgc caacgucagc uuccaggacg acuacggguc ccugagcgcc     1860

aaggccauca gcaagauccu gccguaccuc aaggagggcc acaaguccga caucgcgugc     1920

gaccuggccg gguacaagca cagcgccucc agccugaccc gcgaggagcu cgagaacaag     1980

accuacaagg accggcugga gauccugccc aagaacaccc uccgcaaccc cgugguggag     2040

aagauccuga accagaugau caacgucauc aaccugauca ucgacucccg ggagcucaag     2100

aaguccgcca aggagcgcga ggagcugacc aagagcauca gcgagacgac caagcugcac     2160

cagcaguaca aggacauccu cgcgaaggcc cccuucaacc ugaccaacac gucccggaac     2220

gacaucaucc gcuacaagcu guacgaggag cucaaggaca acggcuacaa gacccuguac     2280

agcaacaagu acaucucccc ggccgcccug uucagcgggg acuucgacau cgagcacauc     2340

aucccccagu cccgccucuu cgacgacagc uucuccaaca agacccugga gcugcggagc     2400

aucaacaucg agaagggcaa caagaccgcc uacgacuuca ucaagggcaa guacggggag     2460

acgggcgagg acaacugccu cgacaaguac cugaaccgga ucgagacccu guacaagaac     2520

gggauccuca agaaguccaa guacaacaag cugaagaugc aggacaagga cauccccgac     2580

ggcuucaucg agcgcgacau ccgggacacc caguacaucg cgaaguacgc caagacccug     2640

cucggcgacc uggugaaguu cgucgugagc accagcgggu ccgugacgga gcgccugcgc     2700

gaggacuggc agcucgucga cgugaugaag gagcugaacu gggagaagua caugcagcug     2760

ggccucaccg aggaguucac caacgaggag gggcaccgga uccgcaagau caaggacugg     2820

accaagcgga acgaccaccg ccaccacgcc auggacgccc ugacgguggc cuucaccaag     2880

cggcaguuca uccaguaccu gaacaaccuc aacgcgcgcc ggaucagcga cgagcagggc     2940

aucuccaaca gcgagaagga cgagcgcaac aacgucucca ucaccaccaa cgacaugaag     3000

cugaacacgc gggacgugcu ggggaucgag aagaaggagc ucuaccgcga caaccggggc     3060

aagcugcgcu ucaaggcccc caugccgcug cucgaguucc gggaggaggc caagcgccac     3120

cuggagaaca cccugaucag caucaaggcc aagaacaagg ugaccaccau caacaucaac     3180

acgcucaaga aggccggcgg ggucuacaag aagacccagc ugacgccccg gggccagcug     3240

cacaacgaga ccaucuacgg gucccagaag caguacaucg ugaaggagga gaccaucaac     3300

gcgaaguucg acguggagaa gaucgccacc gucaccaacc ccguguaccg cgaggcccuc     3360

cugaagcggc uggagcagaa caacaacgac cccaagaagg ccuucaccgg cagcaacgcg     3420

cucacgaaga agccgaugug guucgacgag cagcagaccg agaaggugcc cgagaagguc     3480

aagaccgugu ccuucgagac caucuacacg auccgcaagg agaucagccc cgaccugaag     3540

aucgagaagg uggucgacga gaaggugcgg cagauccuga acaaccgccu caacgaguac     3600

aacgggaacg ccaagcaggc cuucuccaac cuggacgaga accccaucug gcugaacaag     3660

gagaagggca ucaccaucaa gcgggugacc aucaccggca ucaacaacgc ccaggcccuc     3720

cacgagaagc uggacaagga cgggaagccg auccuggacg gcgaggggaa gaagcagccc     3780

gucgacuucg ugaacacggg caacaaccac cacguggcgg ucuacugcga cgccgacggg     3840

aaccuccagg agaacgugau cagcuucuac gaggccguga cccgccgcaa ccugggccug     3900

cccaucaucg acaaggacua caagaaggac gacggcuggc aguuccucuu cuccaugaag     3960

cagaacgagu acuucgucuu ccccaacgag agcaccgggu ucaacccgaa cgagguggac     4020

cugcugaacc ccgacaacua cucccucauc agccccaacc uguuccgggu gcagaaguuc     4080

accaucaagg acuacuucuu ccgccaccac cuggagacga acgucgaguc caacagcacc     4140

cucaagggca ucaccuggcu gcggugcggg cugaacaaca ucgacaacau caucaaggug     4200

cgcaucaacc acaucggcca gaucauccac gugggggagu acuga                     4245


<210>  2153
<211>  4248
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2153
auggccaagu ucaacaagcc cuacagcauc ggccuggaca ucgggguguc cagcgucggc       60

uacgccguga ucaccgagga cuaccgcgug ccggcguucc gguucaaggu ccucgggaac      120

accgagaagg agaagaucaa gaagaaccug aucggcucca cgaccuucgu gcccgcccag      180

agcgcccagg ugacccgcgu cuuccgggug aaccgccggc gcaucgaccg gcgcaaccac      240

cggaucgccu accugcgcga caucuuccag aaggagaucg agaaggugga caagaacuuc      300

uaccggcgcc ucgacgaguc cuuccggguc cugggggaca agagcgagga ccugcagauc      360

aagcagcccu ucuucggcga caaggagcuc gagaccgccu accacaagaa guaccccacg      420

aucuaccacc uccgcaagca ccuggcggac gccgacaaga acuccccggu ggccgacauc      480

cgggaggugu acauggcccu cagccacauc cugaaguacc gcggccacuu ccugacccuc      540

gacaagauca accccaacaa caucaacaug cagaacuccu ggaucaacuu caucgagagc      600

ugccaggagg ucuucgaccu ggaggugucc gaggagagca agaccaucgc ggccaucuuc      660

aaguccagcg agaaccggca ggagaaggug aagcagaucc uguccuacuu ccagcaggag      720

cuccugaaga aggacaagag caucuucaag cagcugcucc agcugcuguu cgggcucaag      780

accaaguuca aggacugcuu cgagcuggag gaggagcccg accugaacuu cuccaaggag      840

aacuacgacg agaaccucga gaaccugcug ggcacgcucg aggaggacuu cagcgacguc      900

uucgccaagc ugaagauccu gcgcgacacc auccuccugu ccgacaugcu gaccuacacc      960

ggggccacgc acgcccgguu cagcgcgacc augguggagc gcuacgagga gcaccggaag     1020

gaccuccagc gcuucaagag cuucgugaag cagaaccugu ccgagcagga cuaccuggac     1080

aucuucggcc gcaagacccc caacggguuc gacgucgaca aggagaccaa gggcuacgug     1140

ggcuacauca gcaacaagau ggugcucacg aacaagcaga agaccaucca gcagaacuuc     1200

uacgacuaca ucuccgggaa gaucaccggc aucgaggggg ccgaguacuu ccugaacaag     1260

aucagcgacg gcaccuuccu gcggaagcuc cgcacgaccg acaacggcgc caucccgaac     1320

cagauccacg ccuacgagcu ggagaagauc aucgagcggc aggggcgcga cuaccccuuc     1380

cugcucgaga acaaggacaa gcugcugucc auccucaccu ucaagauccc cuacuacguc     1440

ggcccccugg ccaaggggaa caacagccgg uucgcgugga ucaagcgcgc caccucccag     1500

gacauccugg acgacaacga cgaggacacg cggaacggca agauccgccc guggaacuac     1560

cacaagcuca ucaacaugga caagacccgg gacgccuuca ucaccaaccu gaucgggaac     1620

gacaucaucc ugcugaacga gaaggugcuc cccaagcgca gccugaucua cgaggaggug     1680

augcugcaga acgagcucac ccgggucaag uacaaggaca aguacggcaa gacgcacuuc     1740

uucgacuccg agcugcgcca ggagaucauc aacaaccugu ucaagaccaa cagcaagcgg     1800

guguccagcg ccaugcuccu gaacuaccug gagaacuaca ccaaccucca ggccgucgag     1860

aucguguccg gcaucgagaa gggcaagagc cugaacucca cccugaagac guacaacgac     1920

cucaagacca ucuucagcga ggagcugcug gacuccgaga ucuaccagaa ggagcucgag     1980

gagaucguga aggucaucac cguguucgac gacaagaagu ccaucaagaa cuaccugacc     2040

aaguacuucg ggcgccugga guuccucaac gaggagcgga ucaaccagcu gagcaagcug     2100

cgcuacacgg gguggggccg guacagcgcg aagcuccugc uggacauccg cgacgaggac     2160

accggguuca accucaugca guuccugcgg aacgacgacg agaaccgcaa ccugagccag     2220

cucaucuccg acagcacccu guccuucgac agcgccauca aguccaucca gagcaagucc     2280

accguggagg acgacgucuu cgacgagauc aagaagcugg ccggcagccc cgccaucaag     2340

cgcggcaucc ucaacuccau caagaucgug gacgagcugg ugcagaucau cggguacccg     2400

ccccagaaca ucgucaucga gauggcccgg gagaacauga ccacggagga gggccagaag     2460

aaggcgaaga cccgcaagac caagcuggag agcgcccuca agaacaucga gaacuccgug     2520

cuggagaacg ggaaggugcc ccacagcgac gagcagcugc aguccgagaa gcucuaccug     2580

uacuaccugc agaacggcaa ggacauguac agccucgaca agaccggguc ccccacgccc     2640

cuguaccggg accgccugga ccaguacgag gucgaccaca ucaucccgua cagcuuccuc     2700

cccaucgacu ccaucgacaa caaggugcug acccaccggg agaacaacca gcgcaagcug     2760

aacaacaucc ccgacaagga gaccguggcc aacaugaagc ccuucuggga gaagcucuac     2820

aacgccaagc ugaucagcca gaccaaguac cagcggcuga cgaccagcga gcgcaccccg     2880

gacggcgucc ucaccgaguc caugaaggcg ggcuucaucg agcggcagcu gguggagacg     2940

cgccagauca ucaagcacgu ggcccggauc cuggacaacc gcuuccucga caccaagauc     3000

aucacccuca agagccagcu gaucaccaac uuccggaaca cguuccacau cgccaagauc     3060

cgcgagcuga acgacuacca ccacgcccac gacgccuacc uggcggucgu gaucgggcag     3120

acccugcuca aggccuaccc caagcuggcc cccgagcuga ucuacggcca ccacgcccac     3180

uucaaccggc acgaggagaa caaggccacc auccgcaagc accucuacuc caacaucaug     3240

cgguucuuca acaaccccaa cagcaaggug uccaaggaca ucuggaacug caaccgcgac     3300

cugccgauca ucaaggacau caucuacaac agccagguca acuucgugaa gcggaccaug     3360

aucaagaagg gggcguucua caagcagaac aucaucccca acgacuccag cgugacgguc     3420

gacaagcgcu uccccaucaa gaacaagaag uaccuggacc cgaagcucua cggcggcuac     3480

uacgagcgga acuccgcccu gagcguguuc aucaucgggc aguccuucaa caagaacaac     3540

aagcugaagg uggucaagga guucuacgac aucaacauca ucgacuaccc ccucuucaac     3600

accgacccca ugagcuucuu caacgacgac uccgagaacg gcuuccugac caagaacaac     3660

cugcagagcg ugcaggccuu cuacaagauc cccaaguacu cccucaucca gaagaacgac     3720

gggacccgca ugcuguucga gagcacgacc aacuuccaca aggccaccca guucaagcug     3780

accgaggccc agaacgagcu guucuuccac augaagcgcc ugcugacgaa gaccgacuuc     3840

auggaccuca aguccgagga gagcaucaag gagucccagg acuucaucau gaaccacaag     3900

aacgaguucg acaacaucgu gaacggccug uucggguacg cggagcggca gcugggcagc     3960

aagagccucu ucacccaggc ccacgaggcc uacaagcugc gcaaguaccc ggucuucucc     4020

cugaacgagg agaccauccg guacuacuac gacaacuucc uccgccuguu cagccugacg     4080

aaguccggca ccagcuccgg guacguggug ggccucuucg aggcccccaa gcugcggaac     4140

gucguguacc gcgcgaccaa ggacgagagc aacgccaccc ugauccacca guccaucacg     4200

gggcucuacg agacccggau cgaccugagc aagcugggcg aggacuga                  4248


<210>  2154
<211>  4248
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2154
augaaccugc gcaacguggc caaggagggc aucaacuaca acaucgggcu cgaccugggc       60

accgggagcg ucggcugggc cgugaccgac gagcaggggg agcuguucuc cuucaagggc      120

aagacgaccu ggggcagccg gaucuucccc accgcggaga ccgccgccga cacgcgccuc      180

aagcgggggc agcgccggcg cuacgcccgg cgccgguggc gccuggaccu gcuccagucc      240

cuguucgagg aggagaucaa gaagguggac ccggaguucu ucacccggcu gcgccagagc      300

caccuccuga aggaggacaa gaccuacgcc gcgauccugu ccagcaaggg cgccacccgg      360

ccccucuucc ccgaggggga ggccgagguc gaguacuaca agaaguaccc cacgaucuac      420

caccuccgca aggcccugau ggaguccaac aaccaggagg acauccggcu gaucuaccug      480

gcguuccaca acaucgugaa ggcccgcggc aacuuccugc accaggacac cccggggcuc      540

accgccagca acgccaacau gggcgacgcc guggagaagc ugugcuccgc gcuggaggac      600

uggugcagcu cccagaacau cgagugcgcc auccggaaga accagaaguc cauccaggac      660

cucauggagg gcaagaccau cgagcccagc gagagcaacg cccugaagga gacguucgac      720

gccucccgca gcucccggaa ggacaaggcc agcguccugg cgggguggau cgccauccag      780

cccggcgagc aguucgagcc caagcaggcc aaggacgccu ccaaggagau cgccaacgcg      840

guguucgggc ucagcgccaa caugaagaag guguucgucg agcuggagga ggaccagaag      900

aucuaccuga acaacgacga gcaggucgag gccuucuaca ccgugugccc ggacgagggc      960

guggcccucu ucgaggcccu gcagagcaug uacuccgcgu acgugcugau gggcauccuc     1020

aagggggccg acggcgggag caucuccagc ugcaaggugg ccgccuacga gcaguacaag     1080

gaggaccuga agcugcucaa gacccugguc cgcgaguucg cgcccaagaa guacgacgag     1140

uucuuccggg gcgacaucuu cgacgccaag aagggguucc gcacccgcaa cuacgacccc     1200

gccacggcca agggcuacac caaguacaac uccgugcggg gcgccagcua cgacgcguuc     1260

aagaaggagg uggagaagcu guucgagggg accgccgccg aggaggacgu ccgcuacacc     1320

gacaugaugc agcgguucga cgagggcacg uuccuccgcc ggcugaagac cuccgacaac     1380

gggagcaucc ccuaccagcu gcaccucgag gagauggugg ccaucaucga gaaccagaag     1440

accuucuacc cguuccugga ggagaacaag gagaaguucg agucccuggu gaccuuccgc     1500

auccccuacu acgucggccc ccucacgcag aagaacgccc ggcccgacaa ccacgggaac     1560

gcgcgcuucg ccuggagcca gcggaaggag ggccaggaga acgcccgcgu guacccgugg     1620

aacugggagg aggugaucga ccggaagcag uccgccgcca aguucaugga gcgccugacc     1680

gggaccugca ccuacauccu ggaggagccc guccuccccc gguacagccu gcuguacgag     1740

gaguacugcc uccugaacga gcugaacggg gcgcgcuggc gggacauggg cggggacugg     1800

caccgcuucg acuacgagac gcggauggac aucaucgagg accuguucaa gaagaagaag     1860

ggcaccguga aguacgccac cgucgccgac uggcucgugc gcgagaagca ccgguccugc     1920

gccgagguga agggcggcca gggcgagacc ggguucgaga gcaagcuguc cagcuacguc     1980

uucuucaagg gcgugcugca cguggacgag aucgacgagg cggacauccc caugaucgag     2040

gagcucaucu gguggaacac gcuguucgag gaccgcucca uccugaagga gagcaucgag     2100

gacaaguacg gggaccgccu caacgacgag cagaucaagg ccaucugcaa gaagcgguuc     2160

accggcuggg gccgccuguc caagaagcug cucugcggga ucaaggccca gaccgacaac     2220

ggcccgugca gcaucaugga cguccugcgg gaggggaacc ccaacaacga cgcccgcagc     2280

gccaccaugg uguucaugga gaucauccgg gacgacaagu ucggcuucac gaagcugauc     2340

gaggaggaga acaagaagcg caucuccagc guggacgacu ucgacgucaa cgaccucccc     2400

gggucccccg cggugcggcg caccgugaac caggccaagc ggaucgucga cgagaucgug     2460

ggcaucgccg gcugcgcccc gcagaacauc uucaucgagg ugacccgcga cgaggacggg     2520

cggaacaagg gccgccggac caagcgccgg uacgacgcca uccaggaggc ggucaaggug     2580

cugaagaagg agcagaagga guacuucgac gccaacgugg ccgaccugcu caagaacacc     2640

gcccccgccg cgcuggacga cgagcgccug gcccucuacu ucaugcagaa cgggaagugc     2700

auguacugcg gcaaggaccu ggacaucaac cagcuggaca aguaccaggu cgaccacauc     2760

cucccccaga gcuacgugaa ggacgacucc uucgagaaca aggcccuggu gcacagcgac     2820

gacaaccagc ggaaguccaa cagcaugcug cucgacgacu ccaucauccg caagaugaag     2880

cccuacuggg aggcccugcg gagcgcgggg cugaucggcg agaagaaguu caagaaccuc     2940

acgcgcgucc ggguguccga caaggagcug ggcggguuca ucgcccgcca gaugguggag     3000

accagccagu ccaugaagcu gaccagccuc cugcuggagc aggccuaccc ggagacccag     3060

guccgccccg ugaaggccgg cuucuccagc gaccuccggc gccggcugaa gcuggugaag     3120

ugccgcgaga ucaacgacua ccaccacgcc cacgacgcgu accucgccug cgaggucggg     3180

caguucaucg cccugcggca caacaagcug uacgagaacc ccaucggcua cucccacgcc     3240

gugaaggacu ucaucgccaa gcgcgcgaag uacgccagcg agcacgggaa gccgauggac     3300

acguacggca ugcccggcag cuccagcuuc gugaucucca gcuucaugac cuccggguuc     3360

gacgaggaga ccggcgagau cuucaaggac gccugggacg ccagccucgc cgcgguccgg     3420

auccaccagg cccugaaccg caaggacugc uacaucuccc ggaugcccga ggagaccagc     3480

ggggccuucu gggacgccac gaucuacucc cccaagcaga ccaagaagaa gcugagccuc     3540

ccgcugaaga agggccugga cccccagaag uacggguccu acagccgcga gcaguucgcc     3600

uacuucuucg uguacgaggc gaagaagauc aagaagaagg aggagaccaa ggaguacgug     3660

uucgccuccg uccccgucca gguggccgcc cucguggcgg ccgaccccaa cgcccugauc     3720

gaguucgcca aggccgacgc ggaggagcgg ggccugaagu ucgccaagau cgugcgcgag     3780

aagguguaca aguaccagcu caucgagguc aacgacgagc gguucuucgu gaccggccug     3840

cgcacggugc ggaacgggcu gcagaucgcc uucagcgagc aggaccgcaa caucgucucc     3900

ggcaucgugg aggggaacag cuccaacaac aaccuccugg acaucuacga caagaucacc     3960

aaccgguacg cccuguacgc cagccgcuuc ggcaagcucc ugaaccuguc cggcuaccgg     4020

gagaacuuca uccaggcgag ccucgaggac caggagcgcg ugguccuggc ccugaucagc     4080

cucuucaacg cccgcaccaa caugaucgac cuguccgcca ucgggagcuc caagaccgug     4140

ggcaacauga gcaugacguu cgccaaggag auggcgaugg ccgacaaccg guucgccuuc     4200

aucgaccagu ccgugaccgg gaugcuggag cgcaagaccu accucuga                  4248


<210>  2155
<211>  4251
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2155
augggcagcc gcaucauccc caugucccag gacauccugg gggaguucgg caaggggaac       60

agcguguccc agaccagcga gcggaccgcc uaccgcucca cgcggcgccu ccgggagcgc      120

uuccugcugc ggcgcgagcg gcuccaccgc guccugaaca uccuggacuu ccucccgacc      180

cacuacgcca gccagaucga cuucaagaag cggcugggca aguucaagga gaacaaggag      240

cccaagaucg cguacgacaa caaguccuuc cuguuccucg acagcuucaa cgagaugcug      300

aaggacuucg agcaggagca gcccaacaug cugaucaacg agaaggggga gaagaagcuc      360

auccccuacg acuggacccu guacuaccug cgcaagaagg cccuccggga gaagaucucc      420

aaggaggagc ugagcuggau cauccugaac uucaaccaga agcgcggcua cuaccagcuc      480

cggggcgagg acgaggagga gaacccgucc aagcuggugg aguuccacag ccugaccgug      540

gucgacgugg ugcccgacga gcccaucaag gggaagcucg agacguggua cuccaucauc      600

cuggacaacg gcugggucua ccgccggagc uccaagaccg agcuguacga cuggaaggac      660

aagaucaagg aguucaucgu gaccaccgac cucaacuacg acgggacggu gaagaccgac      720

aaggagggca acgucaagcg cagcuuccgg gcccccuccg agaccgacug gacccugauc      780

aagaagaaga cggaguacga gaucgagcag agcuccaaga ccaucgggga guacaucuac      840

aacacccugc ucaaguaccc ggagcagaag auccgcggca agcugauccg gaccaucgag      900

cgcaaguacu acaaggagga gcugaagcag auccuccuga agcagacgga guuccacaac      960

gagcugaagg acgacagccu cuacuacgac ugccugcgcg agcuguaccg gaacaacgag     1020

agccaccagc uguuccugga gaagaagaac uucgcccagc uguucaucga ggacaucauc     1080

uucuaccagc gcccccuccg gucccagaag ucccugauca gcaacugccc ccuggaguuc     1140

uacaaguuca aggacagcgu gggcaacgag aaggugcagc accucaaggc cauccccaag     1200

uccaacccgu ucuaccagga guuccgcauc uggcagugga ucagcaaccu guccaucuac     1260

agcaagcugg acgacuccaa cuucaccagc cacuucuucg acuccaccga cgacugggag     1320

agccucuucg agaugcugaa cgggcggaag gagaucgagc agaagcagcu gcucaaguuc     1380

cugcuggaga agaagggcuu caaggggaag ucccugagcu ccgagaucga gaaguaccgc     1440

uggaacuacg ucgaggacaa gaaguacccc ugcaacgaga cccggagccu cauccagucc     1500

cgccuggaca agguggcgaa ccugcccgag agcuuccuca cgcggcacgu ggagcaggag     1560

cuguggcacc ugaucuacuc cgucaccgac aagaucgagu ucgagaaggc ccucgacacc     1620

uucagcaaga aguacaagau cgacaccccc acguucgugg acgccuucaa gaagugcccg     1680

cccuucaaga gcgaguacgg cuccuucagc gagaaggcca ucaagaagcu gcugccccuc     1740

augcgcaccg gcaaguacug ggaccuggac aacaucgaca aggcgaccaa gcagcggauc     1800

gagaagauca ucuccgggga guacgacaag gacauccgcg accgggugcg cgagaaggcc     1860

aucaaccugc ucagcgagac cgacuucaac cggcugcagc uguggcucgc ccaguacauc     1920

gucuacgacc gccacuccga ggccacgagc cuggacaagu gggagaccau cgacgacauc     1980

gugaccuacc ugcacuccuu caagcagcac agccuccgga accccaucgu ggagcagguc     2040

aucaccgaga cgauccgcgu ggugaaggac aucuggucca acuacggcaa cgggagcaag     2100

gacuacuuca acgagaucca cgucgagcug ggccgggaga ugaagcugcc ggccgaggag     2160

cgcaagcgca uccaggagaa gaacucccag aacgaggaca ccaaccuccg gaucaaggcg     2220

cugcugagcg agaugcagaa cgaccccaac guggagaacg uccgccccua cucccccgug     2280

cagcaggaga uccucaagau cuacgaggag ggggugcuga acagcaacau cgagaucgcc     2340

gacgacaucc ugaagaucuc caagaccgcc cagccgagcu ccagcgaccu caagcgguac     2400

aagcuguggc uggagcagaa guaccagagc cccuacaccg gcgagaucau cccccucaac     2460

aagcuguuca cgcccgagua cgagaucgag cacaucaucc cgcagucccg cuacuucgac     2520

gacagcuaca acaacaaggu caucugcgag uccgccguga acaagcugaa ggacaacagc     2580

cucggcuacg aguucaucaa gggguacaac ggccagaucg uggagugcgg guucggcaag     2640

aaggucaaga ucuucuccau cgacgaguac gaggacuucg ugcagaagaa cuacgccaac     2700

aaccggagca aggcgucccg ccugaugaug gccgacaucc ccgagaagau gaucgagcgg     2760

cagcugaacg acacccgcua caucagcaag uacaucucca acauccucag caacaucgug     2820

cgguccgaga gcaacgacga cggggucaac uccaagaacc ugaucagcgu gaacggcaag     2880

aucaccaccg ugcugaagaa ggacuggggc augaacgaca ucuggaacga ccucauccug     2940

ccccgcuuca uccggaugaa ccagaucacc gacuccgaca gcuucacgac cuggaacgag     3000

cgcuuccaga aguucauccc gaccgucccc aucgagcugu ccaagaacuu cagcgccaag     3060

cggaucgacc accgccacca cgccauggac gcccucguga ucgcgugcgc cacccgggac     3120

cacgugaacc ugcugaacaa ccucuacgcc aaguccagcg cccgcuacga ccugaaccgg     3180

aagcugcgca ucuucgagaa gacgaccuac aaggacauca agaccgggca gaucaucgag     3240

cgggagaucc ccaaggccuu ccucaagccc uggcagaacu ucaccacgga cgcgaaggac     3300

gccaucgaga gcaucaucgu cuccuacaag cagagccugc gcgugaucaa caaguccacc     3360

aacuacuacc agaagugggu ggagaaggac ggcaucguca agaaggagaa gaucaagcag     3420

gaggggaaga acugggccau ccggaagccg cugcacaagg acaccgugag cggccucgug     3480

cagcuguccu gggucaaggu gcccaagggg aaggugauga ccgccacgcg caagagccug     3540

gacuccagcu ucgaccucaa gaccaucgcg accaucaccg acacgggcau ccagaagauc     3600

cugaucaagu accuggaguc caagggcaac aaccccgaga gcgccuucuc ccccgagggg     3660

aucgaggacu ucaacaacaa cauccaccuc uacaacgacg gcaagaagca ccgcccgauc     3720

accaaggucc ggcuguacga gaucgggagc aaguuccccc ugggcgagaa gggcaacaag     3780

aacgccaagu acguggaggc cgccaagggg accaaccucu ucuucgcggu guacgccaac     3840

aacaagggcg agcgcuccuu cgagaccauc ccccugaacc uggucaucga ccggcucaag     3900

cuggggcuga gcgccgugcc cgagacgaac gagaagggcg acaaccuccu guuccaccug     3960

uccccgaacg accucgugua cguccccagc gaggacgaga uggagaaccu gaagcugauc     4020

aaccucgagc acaucuccaa ggaccagcug aaccgcaucu acaagauggu gagcuccacc     4080

agcaaccggu gcuucuucau caagagcgag aucgcccugu ccaucgugaa caagaucgag     4140

uacagccccc ucaacaagau ggagaagucc aucgagggga ucaugaucaa ggacgucugc     4200

cugaagcucc aggugaaccg caucggcaac cucgagcggu acuucagcug a              4251


<210>  2156
<211>  4257
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2156
augaagguga agaacauccu gggccucgac aucgggguca ccagcaucgg cuucgcccac       60

gugaccgaga acgagaagga caacaagucc cacaucaagc agcugggggu gcgcaucaac      120

ccccugacgg ucgacgagca gaccaacuuc gagaagggcc ggccggccag cgugaacgcg      180

gaccgcaccc ucaagcgcgg ggcccggaag augcuguccc gguacaagga ccgccggaag      240

aaccugaucg agauccucag caagaccggc uucaucaacu ccgagagcau ccuggccgag      300

gacggcaaga acacgaccca cuccaccugg gcccugcgcg ccaaggcgag ccgggagcgc      360

aucgagaagg acgagcucgg gcgggugcug cuggccauca acaagaagcg cggcuacaag      420

uccagccgga aggccaagaa cgaggacgag gggcaggcca ucgacggcau ggcgaucgcc      480

aagcgccucu acgaggagaa ccugaccccc gggcagcugg ucuaccagcu ccugcgggag      540

ggcaagaagu acauccccga cuucuaccgc uccgaccugc aggacgagcu cgacaaggug      600

uggaacuucc agaagcaguu ccaccaggag auccugacgg acaccuucua caaggacauc      660

cagggcaagg ggcagcgggc caccagcgcc uccuucuacg gcguguacgg guucaacacc      720

gccgagaaca agggcagccg cgaggagaag cggcugcagg cguacgccug gcgcuccgac      780

gcccucacga agcagcugac ccgggaggag gucgccuucg ugaucaccga gaucaacaac      840

aaccugaaca acagcuccgg cuaccucggg gccaucagcg accgcuccaa ggagcuguuc      900

uucaacaaga agaccguggg ccaguaccug uacgagcagc uccagcagaa cccccacacg      960

cgccugaaga accaggucuu cuaccggcag gacuaccugg acgaguucga gaccaucugg     1020

accauccaga gccgcuacua cccggagcuc accgacgacc ugaagcggga gauccgcgac     1080

guggugaucu ucuaccagcg gaagcucaag agccagaagg ggcucgucuc cuucugcgag     1140

cuggagagca aggagaucau gaucgagaag aacggcaagg cggccgugaa gaagaucggg     1200

uccaaggugg cccccaagag cagcccccug uuccaggagu ucaagaucug gcagguccuc     1260

cacaacgugg acaucaagaa caagaaggac aagacgaagc gcccccugga cgacuccgag     1320

aaggaguacc uguucaacga gcucaacauc aagggcaacc ugaaggccgc caaggugauc     1380

cagcugcuca cccugaacaa gaacgagacc gagcugaacu acacccagcu cgagggcaac     1440

cggacgcaga aggcgcugua cgaggccuac cugaagaucc ucgacaccga gggguacgac     1500

gucaacaacc ugcugggcau caaguccaac aaggacgagg uguuccugga cgaccucgac     1560

gugaacgccc uggagaucaa gaccaugauc aagcagaucu ucaacgcccu gaacaucaac     1620

accgggaucc ucgacuucga cgccgagcug gacggcaagg cguucgagca gcaggccagc     1680

uaccaccugu ggcaccuccu guacuccuac gaggaggacg ggagcaaguc cggcaacgag     1740

caccuguaca agcuccugga gaacaaguuc ggcuucaagc cggagcacgc ccgcauccug     1800

gccgggauca cgcucagcga cgacuacggc aaccuguccg cgaaggccau gcggaagauc     1860

uacccccaga ucaaggagaa caacuucagc accgccugcg agcuggucgg guaccgccac     1920

uccaagaaca gccucaccaa ggaggagaac caggagcggc cccugaagaa caagcuggag     1980

cuccugaaga agaacucccu gcgccagccc guggucgaga agauccucaa ccagaugauc     2040

aaccugguga acgagcugau cgacaagaac agcgagaagg acgagcaggg cuccaucauc     2100

gaguacuucc gguucgacga gauccgcauc gagcucgccc gggagcugaa gaagaacacc     2160

gacgagcgcc gggaccugga cgccgcgauc cgcgagggga accuccggaa cgagaagauc     2220

uacaagcugc ugcaggccca guucggcauc aagaacccga cgcgcaacga caucauccgc     2280

uaccggcucu acgaggagcu gaagaacaac ggcuacaagg accuguacac caacagcuac     2340

aucccccagg agaagcuguu cgggaaggag aucgacaucg accacaucau cccccagagc     2400

aagcuguucg acgacuccuu cagcaacaag acccugaccu acaacacgac caacaaggac     2460

aagggcaacc agaccggguu cgacuacauc gaguccaagu acggcaucga cggcaucgag     2520

gccuacaccg cccgcaucga gaaccucuuc gagagcggga agaagaacaa ggaggagggc     2580

guguccaagg ccaaguacca gaagcugcug aagaaggaga gcgagaucgg ggacggcuuc     2640

aucgagcggg accuccgcga gucccaguac aucgcgaaga aggccaagga gcugcugcuc     2700

cagaucaccc ggagcgucgu ggccacgucc gggagcauca ccgcccgccu gcgggaggac     2760

ugggaccugg ugaacgucau gaaggagcuc aaccugccca aguaccgcgc ccugggccuc     2820

accgagaugg aggagcggaa guacggccag aagguggagg ugaucaagga cuggaccaag     2880

cgcgacgacc accggcacca cgcgauggac gcccugacga ucgccuucac caagcacaac     2940

cacauccagu accugaacca ccucaacgcc cgcaaggacg agcagcacaa ggagcacaag     3000

aucaucaacg cgaucgagaa caaggagacc auccugauca ccgacaaguc cgggaaccgg     3060

aagcgcgucu ucaaggcccc gaugcccaac uuccggcaga cggccaagca guaccuggag     3120

gaggugcuca ucagccacaa ggccaagaac aaggugguca ccaagaacaa gaacaagauc     3180

cugggcaagg agaagaucca ggagacccug acgccccgcg ggcagcucca cgaccagacc     3240

aucuacggcu ucaacuggug gucccggauc acccugagca agaagguguc ccgcaacgag     3300

aucgagaaga ucaucgacaa caacaccaag uucuuccugc aggaguucau ccagcucaag     3360

gggagcaucg ccaaggcguu cuccgacgcc gagcugaaca agcugaugua caagggcaag     3420

auccucaagg agaucgaggu ggccaucccc ugcuacaccc agcggaucga caucagcaag     3480

gucuucacgg acagccagaa guccaacagc gccaaggaga agaacaucaa gaacauccug     3540

gaccugaagg ugcgcaacau ccuccagaac cggcuguccg aguucaaggg cgacuucaag     3600

aaggccuuca gcgaccugga caagaacccg aucugguuca acaaggagaa ggggaucucc     3660

aucaagcgcg ugaucaucag cggcgucaag aacguggagc cccuccacua caagaaggac     3720

caccugggga acgacauccu ggacggcaag gagaaccgca uccccgugga cuucaucucc     3780

accgggaaca accaccacgu cgcgaucuac caggacgaga agggcaaccu ccaggagaag     3840

guggugagcc uguucgacgc cgucaagcug gugaacgccg acgagcccgu gaucgacaag     3900

accuacaacc agggccucgg guggcaguuc cuguucacca ugaagcagaa cgagauguuc     3960

gucuucccga acgacaacac gggcuucgac cccaaggaga ucgaccugcu cgaccccucc     4020

aacaagaagc ugaucagccc caaccuguuc cgggugcagu ccaucagcau cgugcaguac     4080

gggaacaaca ucguccgcga cuucaaguuc cggaaccacc ucgaguccgu gcugaacgac     4140

gacaaggugc ugcacggcau caccuuccag cagaucaaga gccucgggcc gcugcugaac     4200

aucgucaagg ugcgcaucaa ccaccucggc gacaucgugu ccgucggcga guacuga        4257


<210>  2157
<211>  4260
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2157
auggccaagc cccagaacua caccaucggc cuggacaucg ggaccaacag cgugggcugg       60

acggucaccg acgaccagaa caacauccuc cgcaucaagu ccaagaaggc caucggggcg      120

cggcuguucg aggagggcaa gguggccgcc gagcgccgga gcuuccgcac cacccggcgc      180

cggcuguccc gccggaagug gcgcaucaag cuccuggagg agcuguucga cgaggagauc      240

agcaaggugg acccgaacuu cuucgcccgg ucccacgaga gcuggaucuc ccccaaggac      300

aagcgcgagc gguacagcgc caucgucuuc cccacgcccg aggaggacaa gaaguuccac      360

aacgaguacc cgaccaucua ccaccuccgc gaccggcuga ugaaggacga ccagaagcac      420

gacauccgcg agaucuacgu ggcgauccac cacaucauga aguaccgggg gaacuuccug      480

cacgacgagu ccaucgagac cuaccgcagc ggcaugucca gccucggcgg gggcgacgag      540

cggaccaucc ugaacacgaa gauccuggac gaccucaacg acgccuucgc cgacaaccug      600

gccacccagg ggaucuccgu gaacagcgcg uccgccgacc agaucaacga gaucgucacc      660

agcggccacc ugaacgugga cucccagaag gccaucgcca agcuccugcu gcccaccacg      720

cucagcuccg acgacaagaa gcaggacaag aagaucaaga agcuggccac cgacgcggcc      780

aagaacgugg ccagcgccug gcugggguac aaggccaacu ucgcggccau ccucaacauc      840

caggaggucg acaaggacaa gaagaagauc uacagcuucg cccugcaggg cgccgacgag      900

gaggacaagg uggagacccu ggaguccgug cucagccagg accagaucga gaucgucgac      960

cgcaucaucc ggauccgcca cgccaucgug cugggcgaga ucauccccgu ggggaugacc     1020

cuguccgagg cgaugaucga gaaguacgac cagcacgcca aggaccucaa gaaccugaag     1080

acggucaucc gcaaccugaa ggaccggaag aacgccguga agcuccaggc caucuacgac     1140

cuguacguga acaaccgcca cgccgacacc gcgaaggcca ugaagcuggc cggcgucaag     1200

aagcggaccg agcuccugga gcccgaggag uucaacaaga gcaucgagaa gcugcucgac     1260

gacuccccgg ugagcgccga gaucaagaag cgcaucgcgg agaaggacuu ccugccccug     1320

cagcggucca acagcaacgg ggugaucccc aaccagcucc accagaucga gcuggacgag     1380

aucaucaaga agcaguccaa guacuacccc uuccugggcg agaccaacga gcacgccagc     1440

gccgacgucg cccgcaaggc cccgacgaag cucgaccagc ugcugaccuu ccgggugccc     1500

uacuacgugg gcccccucau caccaaggag gagcaggagg agcaggacgg gcacuccuuc     1560

gcguggauga uccgcaagaa ccccaaggac cacgagaaga ucaccccgug gaacuucgac     1620

gagaaggucg accggauggc cagcgccacg caguucauca agcgcaugac caccaaggac     1680

accuaccugc ugggcgagga cgugcucccc uccagcuccc ugaaguacca gcuguucacg     1740

guccugaacg agcucaacaa ccugcggauc aaccacaaga agcugacccg cgaggagaag     1800

gagcagguca ucgaggaccu cuucaagaag uacaagaccg ugaaggccga caaguucaag     1860

aacuacuggc agaaggggca cgccggccug gacgugaaga uccaggggau ggcggacccc     1920

gagaaguuca acagcaccau guccacguac aucgaccugc agaagaucuu cggcgaccag     1980

cucgacgacg cccagcggca gaacgacuuc gagaagauca ucgaguacag caccaucuuc     2040

gaggaccgca agauccuggg cgagaagcug cgggccgacu ucguguggcu cuccgaggac     2100

cagaucaagc gccugugccg gcugcgccuc cagggguggg gccggcugag ccugaagcuc     2160

cugacccgcc ugaccgacgc cgacgggcag aacgugcucg agcgccugcu gaacagcaac     2220

gacaacuuca ugcagaucgu caaggacccc gugaucaagg ccaagaucga gcagaagaac     2280

cagaagcucc ugcgggcgga cugguccgag aacgccacgg agagcauccu ggacgaggcc     2340

uacaccuccc cgcagaacaa gaaggccauc cgccagguga ugcgggucgu gcacgacauc     2400

gugaaggcgu gcgacggcaa ccuccccgcc aaguucgcca ucgaguucgc ccgcagccag     2460

gacgacgacc ccaagcggac cgucgcccgc gcggaccggc ugcagaagau cuacgcccag     2520

aucuccgacg agguggugag ccaggggguc aaggaccagc uguccggcau gaagucccuc     2580

aaggaccgcu acuaccugua cuucaugcag ggcgggcggg acgccuacac cggcaagccc     2640

cugaacaucg accgccucag cgacuacgac aucgaccaca ucaugccgca gagcuucauc     2700

aaggacgacu cccuggacaa ccgggugcug gugacccgcg ccgucaacaa ccagaagucc     2760

gacaacgugc ccgugcuccu guacgggaac caccuggucg ccgaccucgg caucacgauc     2820

cgggagaugu gggaccgcug gcagaagucc gggaugauca gcaagcggaa gcuggcgaac     2880

cugcucaccg accccgccac ccugagcgag uaccgcgccg agggcuucau caaccggcag     2940

cugguggaga ccagccaggu gaucaagcuc acggccacca uccugcaguc cgaguucccc     3000

gacagcaaga ucaucgaggu cccggccaag uacaacagca uccugcgcaa gcaguucgac     3060

cucuacaagu cccgggaggu gaacgacuac caccacgcga ucgacgccua ccugagcacc     3120

aucgugggca acuaccugua ccaggucuac cccaaccucc gccggcuguu cguguacggg     3180

gaguucaaga aguucuccca cggcgaggag gagagcuccc accaggccgu gcgccgcguc     3240

aagagcauga acuuccugga cgaccuccug cgggggaagc acggcgacga caucuacugc     3300

cgcgugaccg gcgaggugau cuucucccgg aaggacauca ucagcaagcu gaagcaggcc     3360

uacuccuuca agcagaugcu cgucacgcag gagguguuca ccaagaaguc cgcgcuguuc     3420

gaccagaccg uguaccccag cccggcccgc gacagcaaga cccgguccgg gcugaucccc     3480

cgcaagcagg gcauggacac ggagaucuac gggggcuaca gcgggaacaa ggacgccuac     3540

uucguccucg cccagguggu gaaguccaag ggccacaccc ugcaggucgu gggcgugccc     3600

auccgggccc ugcgcgcgcu cgccaacugc agcaacuacu ccgagcagcu gcugaagauc     3660

gucaaggccc agguccucuu caacaagaag accgggaagg ccaagaaggg caucagcgac     3720

gugcggaucc ugcuggacaa gauccccuac cgccagccgg ugcucgacgg guccgccuuc     3780

uacaugcugg gcagcagcac cuacaaguac aaccugaagc agaucucccu cagccagcag     3840

uccaugaagu acauccugga cuacaucgac gaccccaacu ucaacaagca cgagaugacg     3900

aagaucgacc agcagaacga gaaggagugc cugcucagcg uguacgacga gauccuggac     3960

aagauggaca aguaccuguc ccucuacgac aucaacgggu uccggaagaa gcugcacgug     4020

ggccgcgaca aguucaucaa gcuggagguc gagaagcagg cggaggccau caagaacgug     4080

cuccggggcu uccacgccaa cagcgacaug accaagcugu ccgaccuggg gcucagcacc     4140

ucccugggcu ucaugcaggc cagcgacggg aucaagcugu ccgacaacgc cguguucauc     4200

uaccagagcc ccaccggccu cuucgagcgc cgggucaagg uguccgaccu gcugaaguga     4260


<210>  2158
<211>  4260
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2158
augaugauca agaacauccu gggccucgac cuggggacca acagcaucgg cugggcccug       60

aucaagcaga acuucgagaa caaguacggg gagauccucg gcaugggguc ccgcaucauc      120

cccaugagcc aggacgugcu gggcgaguuc ggcaagggga acuccgucag ccagaccgcc      180

gcgcggacgg gcuaccgcgg ggugcggcgc cugcgggagc gcuuccuccu gcggcgcgag      240

cggcugcacc gcgugcucaa cguccugaac uuccugccgg agcacuacgc cucccagauc      300

gacuucgaga agcgguucgg caaguucaag uacgagaccg agcccaagcu cgccuacaac      360

aacgacgggu ucuucuucaa gaacagcuuc gacgagaugc ugggcgacuu caaggaccac      420

caguuccacc uccuggagaa cggccgcaag gugcccuacg acuggaccau cuacuaccug      480

cggaagaagg cccuguccca gaagaucgag aaggaggagc ucgccuggau ccugcugaac      540

uucaaccaga agcgcgggua cuaccagcuc cggggcgagg acuucgagga ggagaaggac      600

cgcguguucg uccggcugaa gguggaccgc guggucgaca gcggggagaa caucaagggc      660

aagauccugu acgacgugua cuucgagaac ggcuggaagu acgagaagca gaucgugaag      720

accgaggacu ggaucgggcg gacgaaggag uucaucguca ccgaguccau ccucaagaac      780

ggcgacacca agcgcaccuu caaggcggug gacagcgaga aggacuggau cgccaucaag      840

acgaagaccg agcaggagau cgacaagucc cacaagaccg ugggggccua caucuacgag      900

gcccugcugc agaaccccaa gcagaagauc aagggcaagc ucguccggac caucgagcgc      960

aaguucuacc gcgaggagcu gaagaagauc cuggagcggc agaaggaguu ccacccggag     1020

cuccagagcg acgaccugua caacgacugc auccgcgagc uguaccggaa caacgaggcg     1080

caccagauca cgcucuccaa gaaggacuuc gugcaccucc ugcugaacga ccugaucuuc     1140

uaccagcgcc cccugcggag ccagaagucc agcgugucca acugcacccu cgaguuccgc     1200

aaguacaagg acgagaacgg ggucgagcac gcccaguacc ugaagaccau ccccaagagc     1260

aaccccuacu accaggaguu ccgggugugg caguggaugu ucaaccugaa caucuaccgc     1320

aaggacgacg acaccaacgu gacgaaggag uuccucucca gcaccgagga cuacgagacc     1380

cuguucgagu uccugaacaa ccggaaggag gucgaccaga aggcccuccu gcgccacuuc     1440

aagcugaacg agaagaccca ccgguggaac uacguggagg acaagaagua cccgugcaac     1500

gagacgaaga ccaugaucag cacccgccuc gagaaggugg agaacuaccc cuccgacuuc     1560

cugacccggg agauggagca gaacaucugg cacaucaucu acagcgucaa cgacaagauc     1620

gaguacgaga aggcccugaa guccuucgcc cacaagaaca accuggacga gaacagcuuc     1680

uucgaggcgu ucaagaaguu cccgcccuuc aaguccgagu acggcagcuu cuccgagaag     1740

gccaucaaga agcuccugcc ccugcuccgc cugggcaagu acuggaacug ggagagcauc     1800

gaccggaagu cccaggggcg caucgagaag aucaucacgg gcgaguucga cgggaacauc     1860

aaggacaagg ugcgggagaa ggccauccac cugaccgccg agaacgacuu ccagggccuc     1920

cagcuguggc uggcccagua caucgucuac gggcgccaca gcgaggcguc caugaucggc     1980

aaguggaaca gcgccgacga ccucgaggag uuccugaagg gcuucaagca gcacucccug     2040

cggaacccca ucguggagca ggucaucacc gagacccucc gcguggugaa ggacaucugg     2100

cugaaguacg ggaacggcgc caaggacuuc uucagcgaga uccacaucga gcuggggcgc     2160

gagaugaagc agacgaagga cgagcgggug gacgccacca agcgcaucac cgagaacgag     2220

aacaccaacc uccggaucaa ggcccugcug gcggagauga ugaacgacaa cuccgucgag     2280

aacauccgcc ccuacagccc caugcagcag gagauccuca agaucuacga ggacggcauc     2340

cugaaguccg acaucgagau cgacgacgac auccugaaga ucagcaagac ggcccagccg     2400

uccagcagcg accucaagcg guacaagcug uggcuggagc agcgcuacaa gucccccuac     2460

accgggcaga ucaucccccu caacaagcug uucaccagcg aguacgagau cgagcacauc     2520

aucccccagu cccgguacuu cgacgacagc uucuccaaca agaucaucug cgagagcgcc     2580

gugaacaagc ugaaggacaa cuacaucggc cucggcuuca ucaagcaguu cgggggcaug     2640

aucguggagu gcggguucgg caaguccguc gagaucuuca aggccgagga guacgaggac     2700

uucgugaaga agcacuacgc caacaaccgc agcaagcgga acaagcugcu gcucgaggag     2760

aucccggaga agaugaucga ccgccagcug aacgacaccc ggcacaucuc caaguacauc     2820

agcggcgugc uguccaacau cguccgccag gaggacggga cggacgaggg cgugaacagc     2880

aagaacaucg ugcccgggaa cggcaagauc accacccagc ucaagcagga cugggggcug     2940

aacgacgucu ggaacgagcu gauccucccc cgguucgagc gcaugaacca gcugaccaac     3000

uccaccgacu ucacgagcug gaacaagaac caccagaagu uccugcccac cgugccgauc     3060

gaguucucca agggcuucag caagaagcgg aucgaccacc gccaccacgc gauggacgcc     3120

cucgugaucg ccugcgccac caaggaccac aucaaccugc ugaacaacca guccgccaag     3180

agcgacacca agcgguacga ccucaagcgc aagcugauga aguucgagcg ggucguguac     3240

agccacucca agacgggcga gaagaucgag aaggaggugc ccaagcaguu ccugaagccc     3300

ugggacaacu ucaccgucga cgcgaagaac aagauggaga ccaucaucgu gagcuucaag     3360

cagaaccucc gcgugaucaa caaggccacc aaccacuacg agaaguggau cgagaaggac     3420

gggaucaagc ggaaggagcu gaucaagcag gagggcacga acugggccau ccgcaagccc     3480

augcacaagg agaccgucuc cgggaaggug gaccugccgg gcaucaagau ccccaagggg     3540

aagauccuca ccgccacccg caagagccug gacacguccu ucgaccugaa gaccaucaac     3600

agcaucaccg acaccggcau ccagaagauc cucaagaacu accuggaguu caagggcucc     3660

agcgagcugg cguucucccc cgaggggauc gaggacauga acaagaacau cgagaaguuc     3720

aacgacggca agccgcacca gagcaucaac aaggugcgga ucuucgagcu cggguccaag     3780

uucagcgucg gccagacggg gaacaagaag gacaaguacg uggaggccgc caagggcacc     3840

aaccuguucu ucgccgugua cgaggacaag aacggcaagc gcuccuacga gaccaucccc     3900

cugaacgagg ucaucgagcg gcagaagcag gggcucgaca gcguguccuu caagaacaag     3960

gaggacuucc ugcugugccc caacgaccuc guguacgucc ccagcgagga cgagcuggag     4020

aacaucuaca acaucgacuu caagaaccug accacggaga agaccggcaa caucuacaag     4080

gugguguccu ucagcgggaa ccagaucuuc uucauccgcc aggacaucgc caucuccauc     4140

gucaacaagg cggaguucag cacccucaac aagauggagc gggccaucga cggcagcaug     4200

aucaaggaga acugcaucaa gcugaagauc gaccgccugg ggaacaucgc caagaucuga     4260


<210>  2159
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2159
auggccaagc ccaaggacua caccaucggc cuggacaucg ggaccaacag cgugggcugg       60

gucgugacgg acgaccagaa caacauccuc cgcaucaagg ggaagaaggc caucggcgcg      120

cggcuguuca ccgaggggaa gguggccgcc gagcgccggu ccuuccgcac cacccggcgc      180

cggcugagcc gccggcgcug gcggaucaag augcucgagg agcuguucga cgaggagauc      240

gccaaggucg acccguccuu cuucgcccgc cugcacgaga gcuggaucuc ccccaaggac      300

aagcggaagc gcuacagcgc gaucguguuc cccucccccg aggaggacaa gaaguuccac      360

gagagcuacc cgacgaucua ccaccuccgg gacaagcuga ugaaggacga ccagaagcac      420

gacauccgcg agaucuacau cgccgugcac cagaugauca aggcccgggg caacuuccug      480

cacgacgagu ccgucgagac cuaccgcagc ggcaugucca gccucggggg ccgguccgag      540

cgcaccaucc ugagcgugca gacccuggag gagcucaacg accuguucgc cgagaacgag      600

gggacggagg agguggagcu gaacgucgcg uccgccgagc agaucaacga cauccucacc      660

ggcgggcacc ugaacgccga cagccagaag gagaucucca accugcuccu gcccagcucc      720

uuccccagcu ucgacgacaa ggccaaggag aagcagguca agaagcugau caacaacgug      780

gccaccaaca ucagcaaggc guggcucggc uacaaggccg acuucuccac cauccugaac      840

cuggccaagg uggacaagga ccagaagaag aucuucgccu ucgcccucca gggcgaggac      900

gaggaggaca aggugcagga gcuggagagc cugcucgagc agucccagac ggacaucgug      960

gaccggcuga ucgagauccg ccacgcgauc guccugagcg agaucgugcc cguggggaug     1020

acccucuccg aggccaugau cgacaaguac gaccagcaca aggaggaccu gaucacccug     1080

aaggccguca uccgcaacac caaggacaag aagaaggccg ccaagcucca ggcgaucuac     1140

gaccuguacg ugaagaaccg gcacgccgac cuggccaagg ccaugaagcu cacgggcauc     1200

aagaagcgca gcgagcugcu ggacccggag gagcucaaga aggggaucuc cagccugcug     1260

gacgacuccc ccgaggccgu ggagaucaag cagcggcucg aggagaagac cuuccugccc     1320

cugcagcgca gcaacaacaa cggcgucauc cccaaccagc uccaccaggu ggagcuggac     1380

gagaucauca agaagcaguc caaguacuac ccguuccugg cggagaagaa ccccgacgag     1440

agcgaggagg cccagaagaa ggcccccacc aagcucgacg cccugcugac cuuccgggug     1500

cccuacuacg ucggcccgau gaucacgaag gaggagcagg aggcgcagaa cgggcacucc     1560

uucgccugga uggugcgccg ggacccccag gaccacgagg ccaucacccc cuggaacuuc     1620

gagaagaagg ucgacaagau ggccagcgcc acccaguuca ucaagcgcau gaccacgaag     1680

gacaccuacc ugcucggcga ggacgugcug cccgcgucca gccugaagua ccagcuguuc     1740

accgugcuca acgagcugaa caaccuccgg gucaacggga agaagcugac cuccgacgag     1800

aaggagcagg ucaucgaggg ccuguucaag aagcagaaga cggugaaggc cgacaaguuc     1860

gugaaguacu ggcaggccaa gcacaucggg gccgacauca aggugaaggg ccucagcgac     1920

ccguccaagu ucaacagcac caugagcacc uacaucgacu ucaagaagau cuucggcgac     1980

cagcugaacg acgugaaccg ccagaacgac uucgagaaga ucaucgagua cuccaccauc     2040

uucgaggacc ggaagauccu ggaggacaag cuccgcgccg acuucgcgug gcugagcgag     2100

gagcagauca agcagcugug ccggcuccgc cugcaggggu ggggccggcu guccgacaag     2160

cuccugacgc gccugaccga cgccgacggg cagaacgucc ucgagcggcu guggaacagc     2220

aacgacaacu ucgugcagau cguguccgac cccaacauca aggccaagau cgagcaggag     2280

aaccagaagc ugcuccgcga cagccagucc gacaacgccg ucgagagcau ccuggacgac     2340

gccuacacca gcccccagaa caagaaggcg auccgccagg ugaugcgggu gguccacgac     2400

aucgugaagg ccugcgacgg caacgugccc gccaaguucg ccaucgaguu cgcgcgcagc     2460

caggaggacg acccgaagcg gaccacggcc cgcgucaacc agaugcagaa gaucuacgac     2520

cagaucuccg acgagguggu gagccagggg gucaaggagc agcuguccgg caugaagucc     2580

cucaaggacc gguacuaccu guacuucaug cagggcgggc gcgacgccua caccggcgcc     2640

aagcugaaca ucgaccggcu cuccgacuac gacguggacc acaucaugcc ccagagcuuc     2700

gugaaggacg acucccugga caaccgcguc cugguggccc gggcggugaa caaccagaag     2760

uccgacaagg uccccgcccu ccuguucggg aacaaggugg uggccgaccu gggcaucacc     2820

guccgcgaga ugugggacaa guggcagaag cucgggauga ucagcaagcg gaagcugagc     2880

aaccugcuca ccgaccccga cgcccugacc gaguaccgcg cccagggcuu cauccggcgc     2940

cagcuggugg agacgagcca ggugaucaag cucaccgcga ccauccugca guccgaguuc     3000

ccggacagca agaucaucga gguccccgcc aaguacaacu ccaucgugcg gaagcaguuc     3060

gaccuguaca agagccgcga ggugaacgac uuccaccacg ccaucgacgc cuaccucucc     3120

accaucgucg gcaacuaccu guaccaggug uaccccaacc ugcggcgcau guucguguac     3180

ggggaguuca agaaguucag cuccaacgcc gaggagagcg cgcacgacgu cgcccggcgc     3240

gugaagucca ugaacuuccu cgacgaccug cugcggggca cgcacgggga caacaucuac     3300

ugccgcagca ccggcgagau cguguucaac cggaacgaca ucaucuccaa gcucaagcag     3360

gccuacagcu ucaagcagau gcuggucacc caggaggugu ucacccgcaa guccgcccug     3420

uucgaccaga cgguguaccc gagccccgag cgcgacucca agaagcggag cggccucauc     3480

ccccgcaaga aggggaugga ccccgagauc uacggcgggu acgcgcacaa ccaggacucc     3540

uacuucgucc uggccaagau cgagaaggug aagggcuaca cccugcagau cguggggguc     3600

ccgauccgga gccucgagau caucaacaag gccgaggacu ucagcaccgc ccugcuggac     3660

aucaucaagu ccaaggugau guacaacgag aagaccggcc agcccaagcg cggcaucaag     3720

aacguggaga uccucgucaa caagcugccc uaccggcagc ccauccugga gaaggacgug     3780

agcuacaugc ucggguccag cgccuacaag uacaacauga agcagaucac gcugucccgc     3840

cagagcauga aguacauccu ggacuacauc gacgacccga acuucaccaa gcacgagaug     3900

accaugaucg acgagcagaa cgagcgggag ugccuccugu ccgucuacga cgagauccug     3960

gagaaggugg acaaguaccu cccccuguuc gacggcaaca aguuccgcga gaagcugcac     4020

agcgcgcgga aggaguucgu gucccucagc gucgacgacg aggccuccgc caucgagaag     4080

auccugcgcg ggcugcacgc caacagcgag augcccuccc ucgagaaccu gaagaucaag     4140

cccgcccugg gcuucuucca gaugaagaac gggaucaccc ucagcaacga cgcggugcug     4200

cuguaccagu ccccgacggg ccucuucgag cggcgcguga aggucagcga ccugcugaag     4260

uga                                                                   4263


<210>  2160
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2160
auggccaagc ccaaggacua caccaucggc cuggacaucg ggaccaacag cgugggcugg       60

gucgugacgg acgaccagaa caacauccuc cgcaucaagg ggaagaaggc caucggcgcg      120

cggcuguuca ccgaggggaa gguggccgcc aagcgccggu ccuuccgcac cacccggcgc      180

cggcugagcc gccggcgcug gcggaucaag cuccuggagg agcuguucga cgaggagauc      240

gccaaggucg acccguccuu cuucgcccgc cuccacgaga gcuggaucuc ccccaaggac      300

aagcggaagc gcuacagcgc gaucguguuc cccucccccg aggaggacaa gaaguuccac      360

gagagcuacc cgacgaucua ccaccuccgg gacaagcuga ugaaggacga ccagaagcac      420

gacauccgcg agaucuacau cgccgugcac cagaugauca aggcccgggg caacuuccuc      480

cacgacgagu ccgucgagac cuaccgcagc ggcaugucca gccucggggg ccgguccgag      540

cgcaccaucc ugagcgugca gacccuggag gagcugaacg accuguucgc cgagaacgag      600

gggacggagg agguggagcu caacgucgcg uccgccgagc agaucaacga cauccugacc      660

ggcgggcacc ugaacgccga cagccagaag gagaucucca accuccugcu gcccagcucc      720

uuccccagcu ucgacgacaa ggccaaggag aagcagguca agaagcucau caacaacgug      780

gccaccaaca ucagcaaggc guggcugggc uacaaggccg acuucuccac cauccugaac      840

cucgccaagg uggacaagga ccagaagaag aucuucgccu ucgcccugca gggcgaggac      900

gaggaggaca aggugcagga gcuggagagc cuccuggagc agucccagac ggacaucgug      960

gaccggcuga ucgagauccg ccacgcgauc guccucagcg agaucgugcc cguggggaug     1020

acccuguccg aggccaugau cgacaaguac gaccagcaca aggaggaccu gaucacccuc     1080

aaggccguca uccgcaacac caaggacaag aagaaggccg ccaagcugca ggcgaucuac     1140

gaccuguacg ugaagaaccg gcacgccgac cucgccaagg ccaugaagcu gacgggcauc     1200

aagaagcgca gcgagcugcu cgacccggag gagcugaaga aggggaucuc cagccugcuc     1260

gacgacuccc ccgaggccgu ggagaucaag cagcggaucg aggagaagac cuuccugccc     1320

cugcagcgca gcaacaacaa cggcgucauc cccaaccagc uccaccaggu ggagcuggac     1380

gagaucauca agaagcaguc caaguacuac ccguuccugg cggagaagaa ccccgacgag     1440

agcgaggagg cccagaagaa ggcccccacc aagcucgacg cccugcugac cuuccgggug     1500

cccuacuacg ucggcccgau gaucacgaag gaggagcagg aggcgcagaa cgggcacucc     1560

uucgccugga uggugcgccg ggacccccag gaccacgagg ccaucacccc cuggaacuuc     1620

gagaagaagg ucgacaagau ggccagcgcc acccaguuca ucaagcgcau gaccacgaag     1680

gacaccuacc ugcucggcga ggacgugcug cccgcgucca gccugaagua ccagcuguuc     1740

accgugcuga acgagcugaa caaccuccgg gucaacggga agaagcugac cuccgacgag     1800

aaggagcagg ucaucgaggg ccuguucaag aagcagaaga cggugaaggc cgacaaguuc     1860

gugaaguacu ggcaggccaa gcacaucggg gccgacauca aggugaaggg ccucagcgac     1920

ccguccaagu ucaacagcac caugagcacc uacaucgacu ucaagaagau cuucggcgac     1980

cagcugaacg acgugaaccg ccagaacgac uucgagaaga ucaucgagua cuccaccauc     2040

uucgaggacc ggaagauccu ggaggacaag cuccgcgucg acuucgccug gcugagcgag     2100

gagcagauca agcagcugug ccggcuccgc cugcaggggu ggggccggcu guccgacaag     2160

cuccugacgc gccugaccga cgcggacggg cagaacgugc ucgagcggcu guggaacagc     2220

aacgacaacu ucgugcagau cgucuccgac cccaacauca aggccaagau cgagcaggag     2280

aaccagaagc ugcuccgcga cagccagucc gacaacgccg uggagagcau ccuggacgac     2340

gccuacacca gcccccagaa caagaaggcc auccgccagg ugaugcgggu cgugcacgac     2400

aucgugaagg cgugcgacgg caacgucccc gccaaguucg ccaucgaguu cgcccgcagc     2460

caggaggacg acccgaagcg gaccacggcg cgcgugaacc agaugcagaa gaucuacgac     2520

cagaucuccg acgagguggu cagccagggg gugaaggagc agcuguccgg caugaagucc     2580

cucaaggacc gguacuaccu guacuucaug cagggcgggc gcgacgccua caccggcgcc     2640

aagcugaaca ucgaccggcu cuccgacuac gacguggacc acaucaugcc ccagagcuuc     2700

gucaaggacg acucccugga caaccgcgug cugguggccc gggccgucaa caaccagaag     2760

uccgacaagg ugcccgcgcu ccuguucggg aacaaggugg ucgccgaccu gggcaucacc     2820

gugcgcgaga ugugggacaa guggcagaag cucgggauga ucagcaagcg gaagcugagc     2880

aaccugcuca ccgaccccga cgcccugacc gaguaccgcg cccagggcuu cauccggcgc     2940

cagcuggugg agacgagcca ggucaucaag cucaccgcca ccauccugca guccgaguuc     3000

ccggacagca agaucaucga ggugcccgcg aaguacaacu ccaucgugcg gaagcaguuc     3060

gaccuguaca agagccgcga ggucaacgac uuccaccacg ccaucgacgc cuaccucucc     3120

accaucgugg gcaacuaccu guaccaggug uaccccaacc ugcggcgcau guucgucuac     3180

ggggaguuca agaaguucag cuccaacgcc gaggagagcg cccacgacgu ggcgcggcgc     3240

gugaagucca ugaacuuccu cgacgaccug cugcggggca cgcacgggga caacaucuac     3300

ugccgcagca ccggcgagau cgucuucaac cggaacgaca ucaucuccaa gcucaagcag     3360

gccuacagcu ucaagcagau gcuggugacc caggaggugu ucaccaagaa guccgcccug     3420

uucaaccaga cggucuaccc gagccccaag cgcgacggcu ccaagcgcag cgggcucauc     3480

ccccggaagc gcggcaugga ccccgagauc uacgggggcu acgcccacaa ccaggacucc     3540

cacuucgugc uggcgaagau cgagaaggug aagggguaca cccugcagau cgucggcgug     3600

ccgauccgga gccucgagau caucaacaag gccgaggacu ucagcaccgc ccugcuggac     3660

aucaucaagu ccaaggugau guacaacgag aagaccggcc agcccaagcg cgggaucaag     3720

aacgucgaga uccucgugaa caagcugccc uaccggcagc ccauccugga gaaggacguc     3780

agcuacaugc ucggcuccag cgccuacaag uacaacauca agcagaucac gcugucccgc     3840

cagagcauga aguacauccu ggacuacauc gacgacccga acuucaccaa gcacgagaug     3900

accaugaucg acgagcagaa cgagcgggag ugccuccugu ccguguacga cgagauccug     3960

gagaaggugg acaaguaccu cccccuguuc gacgggaaca aguuccgcga gaagcugcac     4020

agcgcccgga aggaguucgu cucccucagc guggacgacg aggcguccgc caucgagaag     4080

auccugcgcg gccugcacgc caacagcgag augcccuccc ucgagaaccu gaagaucaag     4140

cccgcccugg gguucuucca gaugaagaac ggcaucaccc ucagcaacga cgccgugcug     4200

cuguaccagu ccccgacggg ccucuucgag cggcgcguca aggugagcga ccugcugaag     4260

uga                                                                   4263


<210>  2161
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2161
auggccaagu ucaacaagaa cuacagcauc ggccuggaca ucgggguguc cagcgucggc       60

uacgccgugg ugaccgacga cuaccgcguc cccgcguuca aguucaaggu gcucgggaac      120

accgagaagg agaagaucaa gaagaaccug aucggcucca cgaccuucgu gccggcccag      180

agcgcccagg ggacccgggu cuuccgcgug aaccggcgcc ggaucgaccg ccggaaccac      240

cgcaucgccu accugcggga caucuuccag aaggagaucg agaaggugga caagaacuuc      300

uaccgccggc ucgacgaguc cuuccgcguc cugggcgaca agagcgagga gcugcagauc      360

aagcagcccu ucuucggcga caaggagcuc gagaccgccu accacaagaa guaccccacg      420

aucuaccacc uccggaagca ccuggcggac gccgacaaga acucccccgu ggccgacauc      480

cgcgaggugu acauggccau cagccacauc cugaaguacc gggggcacuu ccugacccug      540

gaccgcauca acccgaacaa caucaacaug cagaacuccu ggaucgacuu caucgagagc      600

ugccaggagg ucuucgaccu cgagaucucc gacgagagca agaccaucgu ggcgaucuuc      660

aaguccagcg acaaccggca ggagaaggug aagaagaucc uguccuacuu ccagcaggag      720

cugcucaaga aggacaagag caucuucaag cagcugcugc agcuccuguu cggccugaag      780

accaaguuca aggacugcuu cgagcucgag gaggagcccg accugaacuu cuccaaggag      840

aacuacgacg agaaccugga gaacuuccuc gggagccugg aggaggacuu cuccgacguc      900

uucgccaagc ugaaggugcu ccgcgacacg auccugcuga gcggcaugcu caccuacacc      960

gggaccacgc gggcccgcuu cagcgccacc augguggagc gguacgagga gcaccgcaag     1020

gaccugcagc gcuucaaguc cuucgucaag cagaaccuga gcgagcagga cuaccucgac     1080

aucuucggcc ggaagacccc caacggcuuc gacguggaca aggagaccaa gggguacgug     1140

ggcuacaucu ccaacaagau gguccugacg aacccccaga agcagaagac cauccagcag     1200

aacuucuacg acuacaucag cgggaagauc accggcaucg agggcgccga guacuuccug     1260

aacaagaucu ccgacgggac cuuccuccgc aagcugcgga cgaccgacaa cggcgcggug     1320

ccgaaccaga uccacgccua cgagcuggag aagaucaucg accgccaggg ggaggacuac     1380

cccuuccucc uggagaacaa ggacaagcug cucagcaucc ugaccuucaa gauccccuac     1440

uacgugggcc cccuguccaa ggggaacaac agccgguucg gcuggaucaa gcgcaccauc     1500

ucccaggaca uccucgacga caacgacgag gacacgcgga acggcaagau ccgcccgugg     1560

aacuaccaga agcugaucaa cauggacgag acccgggacg ccuucaucac caaccugauc     1620

gggaacgaca ucauccuccu gaacgagaag guccugccca agcgcagccu gaucuacgag     1680

gaggugaugc uccagaacga gcugacccgg gucaaguaca aggacaagua cggcaagacg     1740

cacuucuucg acuccgagcu gcgccaggag aucaucaaca accucuucaa gaccaacaac     1800

aagcggguga gcuccgccau gcugcuggac uaccucgaga acuacaccaa ccugcaggcc     1860

guggagaucg ucagcgggau cgagaagggc aagucccuga acagcacccu caagacguac     1920

aacgaccuga agaccaucuu cuccgaggag cugcucgaca gcgagaucua ccagaaggag     1980

cuggaggaga ucgugaaggu gaucaccguc uucgacgaca agaaguccau caagaacuac     2040

cugaucaagu acuucgggca ccucgaguuc cugaacgagg agcgcaucaa ccagcugagc     2100

aagcuccggu acaccggcug gggccgcuac agcgcgaagc ugcugcucga cauccgggac     2160

gaggacacgg gguucaaccu gaugcaguuc cugcgcaacg acgacgagaa ccggaaccuc     2220

ucccagcuga ucagcgacuc cacccugagc uucgacuccg ccaucaagag cauccagucc     2280

aagagcaccg uggaggacga cguguucgac gagaucaaga agcucgccgg cucccccgcc     2340

aucaagcgcg ggauccugaa cagcaucaag aucgucgacg agcuggugca gaucaucggc     2400

uacccgcccc agaacaucgu gaucgagaug gcccgcgaga acaugaccac cgaggagggg     2460

cagaagaagg cgaagacgcg gaagaccaag cucgaguccg cccugaagaa caucgagaac     2520

agcguccugg agaacggcaa ggugccccac uccgacgagc agcuccagag cgagaagcug     2580

uaccuguacu accuccagaa cggcaaggac auguacuccc uggacaagac cgggagcccc     2640

acgccccugu accgcgacca gcucgaccag uacgaggugg accacaucau cccguacucc     2700

uuccugccca ucgacagcau cgacaacaag guccugaccc accgggagaa caaccagcgc     2760

aagcucaaca acauccccga caaggagacc guggccaaca ugaagcccuu cugggagaag     2820

cuguacaacg ccaagcugau cagccagacc aaguaccagc ggcucaccac guccgagcgc     2880

accccggacg gcgugcugac cgagagcaug aaggccgggu ucaucgagcg gcagcugguc     2940

gagacccgcc agaucaucaa gcacguggcg cggauccucg acaaccgcuu ccuggacacg     3000

aagaucaucg cccugaaguc ccagcucauc accaacuucc ggaacaccuu ccacaucgcc     3060

aagauccgcg agcugaacga cuaccaccac gcccacgacg cguaccuggc cguggucgug     3120

ggccagaccc uccugaaggc cuaccccaag cuggcccccg agcucaucua cggccaccac     3180

gcccacuuca accggcacga ggagaacaag gcgacgaucc gcaagcaccu guacagcaac     3240

aucaugcggu ucuucaacaa ccccgacucc aaggugagca aggacaucug ggacugcaac     3300

cgcgaccugc cgaucaucaa ggaggucauc uacaacuccc aggugaacuu cgugaagcgg     3360

accaugauca agaagggggc cuucuacaac cagaaccccg ucggcaaguu caacaagcag     3420

aucgccgcca acaaccgcua cccccucaag accaagguga gcucccugga caccagcauc     3480

uacgggggcu acgggccgau gaacuccgcc cugagcauca ucaucaucgc ggagcgguuc     3540

aacgagaaga agggcaagau cgagacggug aaggaguucc acgacaucuu caucaucgac     3600

uacaagaagu ucaacaacaa ccccuuccag uuccucaacg acaccuccga gaacggcuuc     3660

cugaagaaga acaacaucaa ccgcguccug gccuucuacg gggugcccaa guacagccuc     3720

augcagaaga acgacggcac ccggaugcug uucgagucca agagcaaccu gcacaaggcc     3780

acccaguuca agcucacgaa gacccagaac gagcuguucu uccacaugcg ccgccugcuc     3840

accaagucca accugaugga ccucaagagc gaguccgcca ucaaggagag ccagaacuuc     3900

auccugaagc acaaggagga guucgacaac aucagcaacc agcuguccgc cuucagccag     3960

aagaugcugg gggacaccac gucccuccgg aaccugauca agggcuacaa cgagcgcaag     4020

aucaaggaga ucgacagcuc cgaggaggug aucaaguacu ucuacgacaa cuucaucaag     4080

auguucagcu ucgucaaguc cggggcgccc aagggcauca acgacuucuu caacaacaag     4140

uacaccgugg cccggaugcg cccgaagccc gacaagaagu uccugaacgc cacccucauc     4200

caccagagca ucaccggccu guacgagacg cggaucgacc uguccaagcu cggggaggac     4260

uga                                                                   4263


<210>  2162
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2162
auggccaagu ucaacaagaa cuacagcauc ggccuggaca ucgggguguc cagcgucggc       60

uacgccgugg ugaccgacga cuaccgcguc cccgcguuca aguucaaggu gcucgggaac      120

accgagaagg agaagaucaa gaagaaccug aucggcucca cgaccuucgu gccggcccag      180

agcgcccagg ggacccgggu cuuccgcgug aaccggcgcc ggaucgaccg ccggaaccac      240

cgcaucgccu accugcggga caucuuccag aaggagaucg agaaggugga caagaacuuc      300

uaccgccggc ucgacgaguc cuuccgcguc cugggcgaca agagcgagga gcugcagauc      360

aagcagcccu ucuucggcga caaggagcuc gagaccgccu accacaagaa guaccccacg      420

aucuaccacc uccggaagca ccuggcggac gccgacaaga acucccccgu ggccgacauc      480

cgcgaggugu acauggccau cagccacauc cugaaguacc gggggcacuu ccugacccug      540

gaccgcauca acccgaacaa caucaacaug cagaacuccu ggaucgacuu caucgagagc      600

ugccaggagg ucuucgaccu cgagaucucc gacgagagca agaccaucgu ggcgaucuuc      660

aaguccagcg acaaccggca ggagaaggug aagaagaucc uguccuacuu ccagcaggag      720

cugcucaaga aggacaagag caucuucaag cagcugcugc agcuccuguu cggccugaag      780

accaaguuca aggacugcuu cgagcucgag gaggagcccg accugaacuu cuccaaggag      840

aacuacgacg agaaccugga gaacuuccuc gggagccugg aggaggacuu cuccgacguc      900

uucgccaagc ugaaggugcu ccgcgacacg auccugcuga gcggcaugcu caccuacacc      960

gggaccacgc gggcccgcuu cagcgccacc augguggagc gguacgagga gcaccgcaag     1020

gaccugcagc gcuucaaguc cuucgucaag cagaaccuga gcgagcagga cuaccucgac     1080

aucuucggcc ggaagacccc caacggcuuc gacguggaca aggagaccaa gggguacgug     1140

ggcuacaucu ccaacaagau gguccugacg aacccccaga agcagaagac cauccagcag     1200

aacuucuacg acuacaucag cgggaagauc accggcaucg agggcgccga guacuuccug     1260

aacaagaucu ccgacgggac cuuccuccgc aagcugcgga cgaccgacaa cggcgcggug     1320

ccgaaccaga uccacgccua cgagcuggag aagaucaucg accgccaggg ggaggacuac     1380

cccuuccucc uggagaacaa ggacaagcug cucagcaucc ugaccuucaa gauccccuac     1440

uacgugggcc cccuguccaa ggggaacaac agccgguucg gcuggaucaa gcgcaccauc     1500

ucccaggaca uccucgacga caacgacgag gacacgcgga acggcaagau ccgcccgugg     1560

aacuaccaga agcugaucaa cauggacgag acccgggacg ccuucaucac caaccugauc     1620

gggaacgaca ucauccuccu gaacgagaag guccugccca agcgcagccu gaucuacgag     1680

gaggugaugc uccagaacga gcugacccgg gucaaguaca aggacaagua cggcaagacg     1740

cacuucuucg acuccgagcu gcgccaggag aucaucaaca accucuucaa gaccaacaac     1800

aagcggguga gcuccgccau gcugcuggac uaccucgaga acuacaccaa ccugcaggcc     1860

guggagaucg ucagcgggau cgagaagggc aagucccuga acagcacccu caagacguac     1920

aacgaccuga agaccaucuu cuccgaggag cugcucgaca gcgagaucua ccagaaggag     1980

cuggaggaga ucgugaaggu gaucaccguc uucgacgaca agaaguccau caagaacuac     2040

cugaucaagu acuucgggca ccucgaguuc cugaacgagg agcgcaucaa ccagcugagc     2100

aagcuccggu acaccggcug gggccgcuac agcgcgaagc ugcugcucga cauccgggac     2160

gaggacacgg gguucaaccu gaugcaguuc cugcgcaacg acgacgagaa ccggaaccuc     2220

ucccagcuga ucagcgacuc cacccugagc uucgacuccg ccaucaagac cauccagagc     2280

aaguccaccg uggaggacga cguguucgac gagaucaaga agcucgccgg cagccccgcc     2340

aucaagcgcg ggauccugaa cuccaucaag aucgucgacg agcuggugca gaucaucggc     2400

uacccgcccc agaacaucgu gaucgagaug gcccgcgaga acaugaccac ggaggagggg     2460

cagaagaagg cgaagacccg gaagaccaag cucgagagcg cccugaagaa caucgagaac     2520

uccguccugg agaacggcaa ggugccccac agcgacgagc agcuccaguc cgagaagcug     2580

uaccuguacu accuccagaa cggcaaggac auguacagcc uggacaagac cggguccccc     2640

acgccccugu accgcgacca gcucgaccag uacgaggugg accacaucau cccguacagc     2700

uuccugccca ucgacuccau cgacaacaag guccugaccc accgggagaa caaccagcgc     2760

aagcucaaca acauccccga caaggagacc guggccaaca ugaagcccuu cugggagaag     2820

cuguacaacg ccaagcugau cagccagacc aaguaccagc ggcucacgac cagcgagcgc     2880

accccggacg gcgugcugac cgaguccaug aaggccgggu ucaucgagcg gcagcugguc     2940

gagacgcgcc agaucaucaa gcacguggcg cggauccucg acaaccgcuu ccuggacacc     3000

aagaucaucg cccucaagag ccagcugauc accaacuucc ggaacaccuu ccacaucgcc     3060

aagauccgcg agcugaacga cuaccaccac gcccacgacg cguaccuggc cguggucgug     3120

ggccagacgc uccugaaggc cuaccccaag cuggcccccg agcucaucua cggccaccac     3180

gcccacuuca accggcacga ggagaacaag gcgaccaucc gcaagcaccu guacuccaac     3240

aucaugcggu ucuucaacaa ccccgacagc aaggugucca aggacaucug ggacugcaac     3300

cgcgaccugc cgaucaucaa ggaggucauc uacaacagcc aggugaacuu cgugaagcgg     3360

accaugauca agaagggggc cuucuacaac cagaaccccg ucggcaaguu caacaagcag     3420

aucgccgcca acaaccgcua cccccucaag accaaggugu ccagccugga cacguccauc     3480

uacgggggcu acgggccgau gaacagcgcc cuguccauca ucaucaucgc ggagcgguuc     3540

aacgagaaga agggcaagau cgagaccgug aaggaguucc acgacaucuu caucaucgac     3600

uacaagaagu ucaacaacaa ccccuuccag uuccucaacg acaccagcga gaacggcuuc     3660

cugaagaaga acaacaucaa ccgcguccug gccuucuacg gggugcccaa guacucccuc     3720

augcagaaga acgacggcac ccggaugcug uucgagagca aguccaaccu gcacaaggcc     3780

acgcaguuca agcucaccaa gacccagaac gagcuguucu uccacaugcg ccgccugcuc     3840

accaagagca accugaugga ccugaagucc gagagcgcca ucaaggaguc ccagaacuuc     3900

auccucaagc acaaggagga guucgacaac aucagcaacc agcugagcgc cuucucccag     3960

aagaugcugg gggacacgac cagccuccgg aaccugauca agggcuacaa cgagcgcaag     4020

aucaaggaga ucgacuccag cgaggaggug aucaaguacu ucuacgacaa cuucaucaag     4080

auguucuccu ucgucaagag cggggcgccc aaggacauca acgacuucuu caacaacaag     4140

uacaccgugg cccggaugcg cccgaagccc gacaagaagu uccugaacgc cacccucauc     4200

caccagucca ucacgggccu guacgagacc cggaucgacc ugagcaagcu cggcgaggac     4260

uga                                                                   4263


<210>  2163
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2163
auggccaagu ucaacaagaa cuacagcauc ggccuggaca ucgggguguc cagcgucggc       60

uacgccgugg ugaccgacga cuaccgcguc cccgcguuca aguucaaggu gcucgggaac      120

accgagaagg agaagaucaa gaagaaccug aucggcucca cgaccuucgu gccggcccag      180

agcgcccagg ggacccgggu cuuccgcgug aaccggcgcc ggaucgaccg ccggaaccac      240

cgcaucgccu accugcggga caucuuccag aaggagaucg agaaggugga caagaacuuc      300

uaccgccggc ucgacgaguc cuuccgcguc cugggcgaca agagcgagga gcugcagauc      360

aagcagcccu ucuucggcga caaggagcuc gagaccgccu accacaagaa guaccccacg      420

aucuaccacc uccggaagca ccuggcggac gccgacaaga acucccccgu ggccgacauc      480

cgcgaggugu acauggccau cagccacauc cugaaguacc gggggcacuu ccugacccug      540

gaccgcauca acccgaacaa caucaacaug cagaacuccu ggaucgacuu caucgagagc      600

ugccaggagg ucuucgaccu cgagaucucc gacgagagca agaccaucgu ggcgaucuuc      660

aaguccagcg acaaccggca ggagaaggug aagaagaucc uguccuacuu ccagcaggag      720

cugcucaaga aggacaagag caucuucaag cagcugcugc agcuccuguu cggccugaag      780

accaaguuca aggacugcuu cgagcucgag gaggagcccg accugaacuu cuccaaggag      840

aacuacgacg agaaccugga gaacuuccuc gggagccugg aggaggacuu cuccgacguc      900

uucgccaagc ugaaggugcu ccgcgacacg auccugcuga gcggcaugcu caccuacacc      960

gggaccacgc gggcccgcuu cagcgccacc augguggagc gguacgagga gcaccgcaag     1020

gaccugcagc gcuucaaguc cuucgucaag cagaaccuga gcgagcagga cuaccucgac     1080

aucuucggcc ggaagacccc caacggcuuc gacguggaca aggagaccaa gggguacgug     1140

ggcuacaucu ccaacaagau gguccugacg aacccccaga agcagaagac cauccagcag     1200

aacuucuacg acuacaucag cgggaagauc accggcaucg agggcgccga guacuuccug     1260

aacaagaucu ccgacgggac cuuccuccgc aagcugcgga cgaccgacaa cggcgcggug     1320

ccgaaccaga uccacgccua cgagcuggag aagaucaucg accgccaggg ggaggacuac     1380

cccuuccucc uggagaacaa ggacaagcug cucagcaucc ugaccuucaa gauccccuac     1440

uacgugggcc cccuguccaa ggggaacaac agccgguucg gcuggaucaa gcgcaccauc     1500

ucccaggaca uccucgacga caacgacgag gacacgcgga acggcaagau ccgcccgugg     1560

aacuaccaga agcugaucaa cauggacgag acccgggacg ccuucaucac caaccugauc     1620

gggaacgaca ucauccuccu gaacgagaag guccugccca agcgcagccu gaucuacgag     1680

gaggugaugc uccagaacga gcugacccgg gucaaguaca aggacaagua cggcaagacg     1740

cacuucuucg acuccgagcu gcgccaggag aucaucaaca accucuucaa gaccaacaac     1800

aagcggguga gcuccgccau gcugcuggac uaccucgaga acuacaccaa ccugcaggcc     1860

guggagaucg ucagcgggau cgagaagggc aagucccuga acagcacccu caagacguac     1920

aacgaccuga agaccaucuu cuccgaggag cugcucgaca gcgagaucua ccagaaggag     1980

cuggaggaga ucgugaaggu gaucaccguc uucgacgaca agaaguccau caagaacuac     2040

cugaucaagu acuucgggca ccucgaguuc cugaacgagg agcgcaucaa ccagcugagc     2100

aagcuccggu acaccggcug gggccgcuac agcgcgaagc ugcugcucga cauccgggac     2160

gaggacacgg gguucaaccu gaugcaguuc cugcgcaacg acgacgagaa ccggaaccuc     2220

ucccagcuga ucagcgacuc cacccugagc uucgacuccg ccaucaagag cauccagucc     2280

aagagcaccg uggaggacga cguguucgac gagaucaaga agcucgccgg cucccccgcc     2340

aucaagcgcg ggauccugaa cagcaucaag aucgucgacg agcuggugca gaucaucggc     2400

uacccgcccc agaacaucgu gaucgagaug gcccgcgaga acaugaccac cgaggagggg     2460

cagaagaagg cgaagacgcg gaagaccaag cucgaguccg cccugaagaa caucgagaac     2520

agcguccugg agaacggcaa ggugccccac uccgacgagc agcuccagag cgagaagcug     2580

uaccuguacu accuccagaa cggcaaggac auguacuccc uggacaagac cgggagcccc     2640

acgccccugu accgcgacca gcucgaccag uacgaggugg accacaucau cccguacucc     2700

uuccugccca ucgacagcau cgacaacaag guccugaccc accgggagaa caaccagcgc     2760

aagcucaaca acauccccga caaggagacc guggccaaca ugaagcccuu cugggagaag     2820

cuguacaacg ccaagcugau cagccagacc aaguaccagc ggcucaccac guccgagcgc     2880

accccggacg gcgugcugac cgagagcaug aaggccgggu ucaucgagcg gcagcugguc     2940

gagacccgcc agaucaucaa gcacguggcg cggauccucg acaaccgcuu ccuggacacg     3000

aagaucaucg cccugaaguc ccagcucauc accaacuucc ggaacaccuu ccacaucgcc     3060

aagauccgcg agcugaacga cuaccaccac gcccacgacg cguaccuggc cguggucgug     3120

ggccagaccc uccugaaggc cuaccccaag cuggcccccg agcucaucua cggccaccac     3180

gcccacuuca accggcacga ggagaacaag gcgacgaucc gcaagcaccu guacagcaac     3240

aucaugcggu ucuucaacaa ccccgacucc aaggugagca aggacaucug ggacugcaac     3300

cgcgaccugc cgaucaucaa ggaggucauc uacaacuccc aggugaacuu cgugaagcgg     3360

accaugauca agaagggggc cuucuacaac cagaaccccg ucggcaaguu caacaagcag     3420

aucgccgcca acaaccgcua cccccucaag accaagguga gcucccugga caccagcauc     3480

uacgggggcu acgggccgau gaacuccgcc cugagcauca ucaucaucgc ggagcgguuc     3540

aacgagaaga agggcaagau cgagacggug aaggaguucc acgacaucuu caucaucgac     3600

uacaagaagu ucaacaacaa ccccuuccag uuccucaacg acaccuccga gaacggcuuc     3660

cugaagaaga acaacaucaa ccgcguccug gccuucuacg gggugcccaa guacagccuc     3720

augcagaaga acgacggcac ccggaugcug uucgagucca agagcaaccu gcacaaggcc     3780

acccaguuca agcucacgaa gacccagaac gagcuguucu uccacaugcg ccgccugcuc     3840

accaagucca accugaugga ccucaagagc gaguccgcca ucaaggagag ccagaacuuc     3900

auccugaagc acaaggagga guucgacaac aucagcaacc agcuguccgc cuucagccag     3960

aagaugcugg gggacaccac gucccuccgg aaccugauca agggcuacaa cgagcgcaag     4020

aucaaggaga ucgacagcuc cgaggaggug aucaaguacu ucuacgacaa cuucaucaag     4080

auguucagcu ucgucaaguc cggggcgccc aaggacauca acgacuucuu caacaacaag     4140

uacaccgugg cccggaugcg cccgaagccc gacaagaagu uccugaacgc cacccucauc     4200

caccagagca ucaccggccu guacgagacg cggaucgacc uguccaagcu cggcgaggac     4260

uga                                                                   4263


<210>  2164
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2164
auggccaagu ucaacaagaa cuacagcauc ggccuggaca ucgggguguc cagcgucggc       60

uacgccgugg ugaccgagga cuaccgcguc cccgcguuca aguucaaggu gcucgggaac      120

accgagaagg agaagaucaa gaagaaccug aucggcucca cgaccuucgu gccggcccag      180

agcgcccagg ggacccgggu cuuccgcgug aaccggcgcc ggaucgaccg ccggaaccac      240

cgcaucgccu accugcggga caucuuccag aaggagaucg agaagaucga caagaacuuc      300

uaccgccggc ucgacgaguc cuuccgcgug cugggcgaca agagcgagga cauccagauc      360

aagcagcccu ucuucggcaa caaggagcug gagaccgccu accacaagaa guaccccacg      420

aucuaccacc uccggaagca ccuggcggac gccgacaaga acucccccgu cgccgacauc      480

cgcgaggugu acauggugcu gagccacauc cucaaguacc gggggcacuu ccugacccug      540

ggcaagaucg acccgaacaa caucaacaug cagaacuccu ggaucgacuu caucgagagc      600

ugccaggacg ccuucgaccu cgagaucucc gacgagagca agaccaucgc ggccaucuuc      660

aaguccagcg agaaccgcca ggagaagguc aagcagaucc uguccuacuu ccagcaggag      720

cugcucaaga aggacaagag caucuucaag cagcugcugc agcuccuguu cgggcugaag      780

accaaguuca aggacugcuu cgagcucgag gaggagcccg accugaacuu cuccaaggag      840

aacuacgacg agaaccugga gaaccuccug ggcgcccugg aggaggacuu cagcgacgug      900

uucgccaagc ucaagauccu gcgggacacg auccugcucu ccagcaugcu gaccuacacc      960

ggggccaccc acgcgcgcuu cagcgccacg augguggagc gguacgagga gcaccgcaag     1020

gaccugcagc gguucaaguc cuucgucaag cagaaccuca gcgagcagga cuaccuggac     1080

aucuucggcc gcaagacccc caccggcuuc gacguggaca aggagaccaa gggguacgug     1140

ggcuacaucu ccaacaagau gguccugacg aacccccaga agcagaagac cauccagcag     1200

aacuucuacg acuacaucag cgggaagauc accggcaucg agggcgccga gugcuuccuc     1260

aacaagaucu ccgacgggac cuuccugcgc aagcugcgga cgaccgacaa cggcgccauc     1320

ccgaaccaga uccacgccua cgagcucgag aagaucaucg agcgccaggg gcgggacuac     1380

cccuuccugc uggagaacaa ggacaagcuc cugagcaucc ugaccuucaa gauccccuac     1440

uacgugggcc cccucgcgaa ggggaacaac ucccgcuucg ccuggaucaa gcggaccacg     1500

agccaggacg ugcuggacga caacgacgag gacacccgca acggcaagau ccggccgugg     1560

aacuaccaca agcugaucaa cauggacgag acccgcgacg ccuucaucau caaccucauc     1620

ggcaacgaca ucauccugcu gaacgagaag guccugccca agcggucccu caucuacgag     1680

gaggugaugc ugcagaacga gcugacccgc gugaaguaca aggacaagua cgggaagauc     1740

cacuucuuca acagcgagcu ccggcaggag aucaucaaca accuguucaa gacgaacucc     1800

aagcgcguca gcuccgccau gcugcucagc uaccuggaga acuucaccaa ccugcaggcc     1860

guggagaucg ucuccggcau cgagaagggc aagagccuca acuccacccu gaagaccuac     1920

aacgaccuga agacgaucuu cagcgaggag cuccuggacu ccgagaucua ccagaaggag     1980

cuggaggaga ucaucaaggu gaucaccgug uucgacgaca agaaguccau caagaacuac     2040

cucaccaagu acuucgggca ccuggaguuc cugaacgagg agaagaucaa ccagcucagc     2100

aagcugcggu acaccgggug gggccgcuac agcggcaagc ugcuccuggg gauccgggac     2160

gaggacacgg gcuucaaccu gaugcaguuc cuccgcaacg acgacgagaa ccggaaccug     2220

acccagcuga ucagcgacac cacccucucc uucgacagcg cgaucaaggc cauccagucc     2280

aagagcaccg ucgaggacga cguguucgag gagaucaaga accuggccgg gucccccgcc     2340

aucaagcgcg gcauccugaa cagcaucaag aucguggacg agcucgucca gaucaucggg     2400

uacccgcccc agaacaucau caucgagaug gcccgggaga acaugacgac cgaggagggc     2460

cagaagaagg cgaagacccg caaguccaag cuggagagcg cccugaagaa caucgaggac     2520

uccgugcucg agagcggcaa ggugccccac uccgacgagc agcugcagag cgagaagcug     2580

uaccucuacu accugcagaa cgggaaggac auguacuccc uggacaagac cggcagcccc     2640

acgccccucu accgcgacca gcuggaccag uacgaggucg accacaucau cccguacucc     2700

uuccugccca ucgacagcau cgacaacaag gugcucaccc accgggagaa caaccagcgc     2760

aagcugaaca acauccccga caaggagacc guggccaaca ugaagcccuu cugggagaag     2820

cuguacaacg ccaagcucau cagccagacc aaguaccagc ggcugacgac cuccgagcgc     2880

accccggacg ggguccugac cgagagcaug aaggcgggcu ucaucgagcg gcagcucgug     2940

gagacgcgcc agaucaucaa gcacguggcc cggauccugg acaaccgcuu cuccgacacc     3000

aagaucauca cccucaagag ccagcugguc accaacuucc ggaacacguu ccacaucgcc     3060

aagauccgcg agcugaacga cuaccaccac gcccacgacg ccuaccuggc gguggugguc     3120

ggccagaccc uccugaaggc cuaccccaag cuggcccccg agcucaucua cgggcaccac     3180

gcccacuuca accggcacga ggagaacaag gccacccugc gcaagcaccu guacuccaac     3240

aucaugcggu ucuucaacaa ccccgacagc aaggugucca aggacaucug ggacugcaac     3300

cgcgaccucc cgaucgugaa ggacgucauc uacaacagcc agaucaacuu cgugaagcgg     3360

accaugauca agaagggcgc guucuacaac cagaaccccg uggggaagcu gaacaagcag     3420

aucgccgcca acaaccgcua cccccugaag acgaaggucu ccagccucga caccuccauc     3480

uacggcgggu acggcccgau gaacagcgcc cuguccauca ucaucaucgc cgagcgguuc     3540

aacgagaaga agggcaagau cgagaccgug aaggaguucc acgacaucuu caucaucgac     3600

uacgagaagu ucaacaacaa ccccuuccag uuccugaaca acgugagcga ggagggguuc     3660

cucaagaaga acgaccugaa ccgcguccug gcguucuacc ggaucuccaa guacagccuc     3720

augcagaaga ucgacggcac ccgcaugcug uucgagucca agagcaaccu gcacaaggug     3780

acgcaguuca agcucaccaa gacccagaac gagcuguucu uccacaugaa gcggcugcuc     3840

accaagucca accugaugga ccucaagagc gagagcgcca ucaaggaguc ccagaacuuc     3900

auccagacgc accagaacga guucgacaac aucgccgacc gccugagcaa cuacucccag     3960

aagaugcugg gggagaccaa gagccugcag acccucauca acggcuacaa cgagcgcccc     4020

aucaagcaga ucgacaacaa cgaggagacc auccgguacu acuacgacaa cuucaucaag     4080

auguucuccu ucgucaagag cggggccccc aaggacauca ucaacuucuu caacgacaag     4140

uacacggugc cgcgcaugcg gcccaagccc gaccgcaaga ugcuggacgc gacccugauc     4200

caccagucca ucaccggccu cuacgagacc cggaucgacc ugagcaagcu gggcgaggac     4260

uga                                                                   4263


<210>  2165
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2165
augagccgca agaacuacgu ggacgacuac gccaucuccc uggacaucgg caacgccagc       60

gucggguggu ccgcguucac ccccaacuac cggcucgugc gcgccaaggg ccacgagcug      120

aucggggugc ggcuguucga cccggccgac accgccgaga gccgccggau ggcccgcacg      180

acccggcgcc gguacucccg ccggcgcugg cggcuccgcc ugcuggacgc ccucuucgac      240

caggcccuga gcgagaucga ccccuccuuc cuggcccggc gcaaguacag cuggguccac      300

cccgacgacg agaacaacgc cgacugcugg uacggcuccg ugcuguucga cagcaacgag      360

caggacaagc gguucuacga gaaguacccc accaucuacc accuccgcaa ggcgcugaug      420

gaggacgacu cccagcacga cauccgggag aucuaccucg ccauccacca cauggugaag      480

uaccgcggga acuuccuggu cgagggcacc cuggagagcu ccaacgccuu caaggaggac      540

gagcuccuga agcugcucgg ccggaucacg cgcuacgaga ugagcgaggg ggagcagaac      600

uccgacaucg agcaggacga cgagaacaag cugguggccc cggccaacgg ccagcuggcg      660

gacgcccucu gcgccacccg cgggagccgg uccaugcgcg uggacaacgc ccuggaggcc      720

cugagcgcgg ucaacgaccu cucccgggag cagcgcgcca ucgugaaggc caucuucgcc      780

ggccuggagg ggaacaagcu ggaccucgcc aagaucuucg ugagcaagga guucagcucc      840

gagaacaaga agauccuggg caucuacuuc aacaagagcg acuacgagga gaagugcguc      900

cagaucgugg acuccggccu gcucgacgac gaggagcggg aguuccugga ccgcaugcag      960

gggcaguaca acgcgaucgc ccugaagcag cuccugggcc ggagcaccuc cgugagcgac     1020

uccaagugcg ccagcuacga cgcccaccgc gccaacugga accugaucaa gcuccagcug     1080

cggaccaagg agaacgagaa ggacaucaac gagaacuacg ggauccuggu cggcuggaag     1140

aucgacuccg gccagcgcaa gagcgugcgc ggggaguccg cguacgagaa caugcggaag     1200

aaggccaacg uguucuucaa gaagaugauc gagacgagcg accucuccga gaccgacaag     1260

aaccggcuga uccacgacau cgaggaggac aagcuguucc ccauccagcg cgacagcgac     1320

aacggcguca ucccccacca gcuccaccag aacgagcuga agcagaucau caagaagcag     1380

gggaaguacu accccuuccu gcucgacgcc uucgagaagg acggcaagca gaucaacaag     1440

aucgaggggc ugcugaccuu ccgggugccg uacuucgugg gcccccucgu cgugcccgag     1500

gaccugcaga aguccgacaa cagcgagaac cacuggaugg ugcgcaagaa gaagggcgag     1560

aucacccccu ggaacuucga cgagaugguc gacaaggacg ccuccgggcg gaaguucauc     1620

gagcgccugg ugggcacgga cagcuaccug cucggggagc cgacccugcc caagaacucc     1680

cugcucuacc aggaguacga gguccugaac gagcugaaca acgugcggcu cagcgugcgc     1740

accggcaacc acuggaacga caagcgccgg augcgccugg ggcgggagga gaagacccug     1800

cucugccagc gccuguucau gaagggccag acggucacca agcggaccgc ggagaaccug     1860

cuccgcaagg aguacggccg gaccuacgag cugagcgggc uguccgacga gagcaaguuc     1920

acguccagcc ucuccaccua cggcaagaug ugccgcaucu ucggggagaa guacgugaac     1980

gagcaccggg accugaugga gaagaucgug gagcugcaga ccgucuucga ggacaaggag     2040

acccuccugc accagcugcg ccagcucgag ggcaucagcg aggccgacug cgcccugcug     2100

gugaacacgc acuacaccgg guggggccgg cucucccgca agcugcugac caccaaggcc     2160

ggcgagugca agaucagcga cgacuucgcg ccccggaagc acuccaucau cgagaucaug     2220

cgcgcggagg accggaaccu cauggagauc aucacggaca agcagcuggg guucagcgac     2280

uggaucgagc aggagaaccu gggcgccgag aacgggucca gccucaugga gguggucgac     2340

gaccugcgcg ugucccccaa ggugaagcgg ggcaucaucc agagcauccg ccugaucgac     2400

gacaucucca aggccgucgg caagcggccg agccgcaucu uccucgagcu ggccgacgac     2460

auccagcccu ccgggcggac caucagccgc aagagccgcc ugcaggaccu cuaccggaac     2520

gccaaccugg gcaaggaguu caaggggauc gcggacgagc ugaacgccug cuccgacaag     2580

gaccuccagg acgaccgccu guuccuguac uacacccagc ucggcaagga cauguacacc     2640

ggggaggagc uggaccugga ccggcucagc uccgccuacg acaucgacca caucaucccc     2700

caggccguga cgcagaacga cagcaucgac aaccgcgugc uggucgcccg ggcggagaac     2760

gcccgcaaga ccgacuccuu caccuacaug ccccagaucg ccgaccggau gcgcaacuuc     2820

uggcagaucc ugcucgacaa cggccugauc agccggguga aguucgagcg ccugacccgg     2880

cagaacgagu ucuccgagcg cgagaaggag cgguucgugc agcgcagccu cgucgagacg     2940

cggcagauca ugaagaacgu ggccacccug augcgccagc gguacggcaa cuccgcggcc     3000

gugaucgggc ugaacgccga gcucaccaag gagaugcacc gcuaccuggg cuucagccac     3060

aagaaccggg acaucaacga cuaccaccac gcccaggacg cccugugcgu cgggaucgcg     3120

ggccaguucg ccgccaaccg cggguucuuc gccgacggcg agguguccga cggcgcccag     3180

aacagcuaca accaguaccu ccgcgacuac cugcgggggu accgcgagaa gcuguccgcg     3240

gaggaccgga agcagggccg cgccuucggg uucaucgugg gcagcaugcg gucccaggac     3300

gagcagaagc gcgucaaccc gcggaccggg gagguggugu ggagcgagga ggacaaggac     3360

uaccuccgca aggucaugaa cuaccggaag augcugguga cccagaaggu gggcgacgac     3420

uucggcgccc uguacgacga gacgcgcuac gccgccaccg accccaaggg gaucaagggc     3480

auccccuucg acggggcgaa gcaggacacc ucccucuacg gcggguucag cuccgccaag     3540

cccgccuacg ccguccugau cgagagcaag ggcaagaccc ggcuggugaa cgugacgaug     3600

caggaguaca gccuccuggg cgaccgcccg uccgacgacg agcugcggaa gguccucgcg     3660

aagaagaagu ccgaguacgc caaggccaac auccugcugc gccacgugcc caagaugcag     3720

cucauccggu acgggggcgg gcugauggug aucaagagcg ccggcgagcu gaacaacgcc     3780

cagcagcucu ggcugcccua cgaggaguac ugcuacuucg acgaccugag ccagggcaag     3840

gggucccucg agaaggacga ccugaagaag cugcucgaca gcauccuggg cuccguccag     3900

ugccuguacc ccuggcaccg cuucaccgag gaggagcucg cggaccugca cguggccuuc     3960

gacaagcugc cggaggacga gaagaagaac gugaucaccg ggaucgucag cgcccuccac     4020

gccgacgcca agaccgcgaa ccuguccauc gugggcauga cggggagcug gcggcgcaug     4080

aacaacaagu ccggcuacac cuucagcgac gaggacgagu ucaucuucca gucccccagc     4140

ggccuguucg agaagcgggu gaccgucggg gagcucaagc gcaaggccaa gaaggaggug     4200

aacuccaagu accggaccaa cgagaagcgc cugcccacgc ugagcggcgc cucccagccg     4260

uga                                                                   4263


<210>  2166
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2166
auggccaagc ccaaggacua caccaucggc cuggacaucg ggaccaacag cgugggcugg       60

gucgugacgg acgaccagaa caacauccuc cgcaucaagg ggaagaaggc caucggcgcg      120

cggcuguucg ccgaggggaa gguggccgcc gagcgccggu ccuuccgcac cacccggcgc      180

cggaucagcc gccggcgcug gcggaucaag cugcucgagg agcuguucga cgaggagauc      240

gccaaggucg acccguccuu cuucgcgcgc cugcacgaga gcuggaucuc ccccaaggac      300

aagcggaagc gcuacagcgc caucguguuc cccucccccg aggaggacaa gaaguuccac      360

gagagcuacc cgaccaucua ccaccuccgg gacaagcuga ugaaggacga ccagaagcac      420

gacauccgcg agaucuacau cgccgugcac cagaugauca agguccgggg caacuuccug      480

cacgacgagu ccguggagac guaccgcagc ggcaugucca gccucggggg ccgguccgag      540

cgcaccaucc ugagcgugca gacccuggag gagcucaacg accuguucgc cgagaacgag      600

gggaccgagg aggucgagcu gaacguggcg uccgccgagc agaucaacga cauccucacg      660

ggcgaccacc ugaacgccga cagccagaag gagaucucca accugcuccu gcccagcucc      720

uuccccagcu ucgacgacaa ggccaaggag aagcagguca agaagcugau caacaacgug      780

gccaccaaca ucagcaaggc guggcucggg uacaaggccg acuucuccac cauccugaac      840

cuggccaagg uggacaagga ccagaagaag aucuucgccu ucgcccucca gggcggcgac      900

gaggaggaca aggugcagga gcuggagagc cugcucgagc agucccagac cgacaucguc      960

gaccggcuga ucgagauccg ccacgcgauc gugcugagcg agaucgugcc cgucgggaug     1020

acgcucuccg aggccaugau cgacaaguac gaccagcaca aggaggaccu gaucacccug     1080

aaggccguga uccgcaacac caaggacaag aagaaggccg ccaagcucca ggcgaucuac     1140

gaccuguacg ugaacaaccg gcacgccgac cuggccaagg ccaugaagcu caccggcauc     1200

aagaagcgca gcgagcugcu ggacccggag gagcucaaga aggccaucuc cagccugcug     1260

gacgacuccc ccgaggcggg ggagaucaag cagcggcucg agggcaagac guuccugccc     1320

cugcagcgca gcaacaacaa cggcgucauc cccaaccagc uccaccaggu ggagcuggac     1380

gagaucauca agaagcaguc caaguacuac ccguuccugg ccgagaagaa ccccgacgag     1440

agcgaggagg cccagaagaa ggcccccacc aagcucgacg cgcugcugac cuuccgggug     1500

cccuacuacg ucgggccgau gaucaccaag gaggagcagg aggcccagga cggccacucc     1560

uucgccugga uggugcgccg ggacccccag gaccacgagg ccaucacgcc cuggaacuuc     1620

gagaagaagg ucgacaagau ggccagcgcg acccaguuca ucaagcgcau gaccaccaag     1680

gacacguacc ugcucgggga ggacgugcug cccgccucca gccugaagua ccagcuguuc     1740

accgugcuca acgagcugaa caaccuccgg gucaacggca agaagcugac cuccgacgag     1800

aaggagcagg ucaucgaggg gcuguucaag aagcagaaga ccgugaaggc cgacaaguuc     1860

gugaaguacu ggcaggccau ccacaucggc gccgacauca aggugaaggg ccucagcgac     1920

ccguccaagu ucaacagcac gaugagcacc uacaucgacu ucaagaagau cuucggggac     1980

cagcugaacg acgcgaaccg ccagaacgac uucgagaaga ucaucgagua cuccaccauc     2040

uucgaggacc ggaagauccu ggaggacaag cuccgcgccg acuucgccug gcugagcgag     2100

gagcagauca agcagcugug ccggcuccgc cugcagggcu gggggcggcu guccgacaag     2160

cuccugaccc gccugacgga cgccgacggc cagaacgugc ucgagcggcu guggaacagc     2220

aacgacaacu ucguccagau cguguccgac cccaacauca aggccaagau cgagcaggag     2280

aaccagaagc ugcuccgcga cgggcagagc gacgacgcgg uggaguccau ccuggacgac     2340

gccuacacca gcccccagaa caagaaggcc auccggcagg ucaugcgcgu ggugcacgac     2400

aucgucaagg ccugcgacgg caacgugccc gcgaaguucg ccaucgaguu cgcccgcucc     2460

caggaggacg acccgaagcg gaccaccgcc cgcgugaacc agaugcagaa gaucuacgac     2520

cagaucagcg acgaggucgu gucccagggc gugaaggagc agcugagcgg gaugaagucc     2580

cucaaggacc gguacuaccu guacuucaug cagggcgggc gcgacgccua cacgggcgcg     2640

aagcugaaca ucgaccggcu cagcgacuac gacaucgacc acaucaugcc ccaguccuuc     2700

gucaaggacg acagccugga caaccgcgug cugguggccc gggccgucaa caaccagaag     2760

uccgacaagg ugcccgccuu ccucuucggg aacaaggugg ucgccgaccu gggcaucacc     2820

gugcgcgaga ugugggacaa guggcagaag cugggcauga ucagcaagcg gaagcucagc     2880

aaccugcuga ccgaccccga cgcgcucacc gaguaccgcg cccagggguu cauccggcgc     2940

cagcuggugg agaccuccca ggucaucaag cugacggcca ccauccucca gagcgaguuc     3000

ccggacucca agaucaucga ggugcccgcc aaguacaaca gcaucgugcg gaagcaguuc     3060

gaccuguaca agucccgcga ggucaacgac uuccaccacg ccaucgacgc guaccugagc     3120

accaucgugg gcaacuaccu cuaccaggug uaccccaacc ugcggcgcau guucgucuac     3180

ggggaguuca agaaguucuc cagcaacgcc gaggaguccg cccacgacau ggcccggcgc     3240

gucaagagca ugaacuuccu ggacgaccuc cugcggggca cccacggcga caacaucuac     3300

ugccgcucca cgggggagau cguguucaac cggaacgaca ucaucagcaa gcugaagcag     3360

gcguacuccu ucaagcagau gcucgugacc caggaggugu ucacccgcaa gagcgcccug     3420

uucgaccaga ccguguaccc gucccccgag cgcgacagca agaagcgguc cggccugauc     3480

ccccgcaaga aggggaugga cacggagauc uacggcgggu acagcggcaa caaggacgcc     3540

uacuucgucc ucgccgaggc cgugaaggag aagggccaca cccugcagau cguggggguc     3600

cccauccggg cgcugaacac ccucaagaac uccgccaacu acagcgagaa gcugcuggag     3660

aucaucaagc cccaggugau guucaacaag aagaacggca agccgaagaa gggggugaac     3720

gacguccgca uccucaugga caacaucccc uaccggcagc ccgugcugga gggcggggcc     3780

cuguacaugc ucggcagcuc caccuacaag uacagccuga agcagaucac gcugucccag     3840

gagagcauga aguacauccu cgacuacauc gacgacccgu ccuucaacaa gcacaucaug     3900

gugaacaucg accaccagga cgagaagaag ugccugcuga gcgucuacga cgagauccuc     3960

gacaagaugg acaaguaccu gccccuguuc gacaucaacg gcuuccgcaa gaagcuccac     4020

gacgggcggg agaaguucgu gaagcugccc gucgagaagc aggccgaggu ggugaaggac     4080

auccugcgcg gccuccacgc caacuccgag augaccaagc ccagcgaccu ggggcugacc     4140

accgcgcucg gcuucaugca ggccccggac gggaucaaga ucuccgacga cgccgucuuc     4200

aucuaccaga gccccacggg ccuguucgag cggcgcguga agguguccga ccugcucaag     4260

uga                                                                   4263


<210>  2167
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2167
augaccaagu ucaacaagaa cuacagcauc ggccuggaca ucgggguguc cagcgucggc       60

uacgccgugg ugaccgagga cuaccgcguc cccgccuuca aguucaaggu gcucgggaac      120

acggagaagg agaagaucaa gaagaaccug aucggcucca ccaccuucgu gagcgcgcag      180

ccggccaagg ggacccgggu cuuccgcgug aaccggcgcc ggaucgaccg ccggaaccac      240

cgcaucacgu accugcggga caucuuccag aaggagaucg agaaggugga caagaacuuc      300

uaccgccggc ucgacgaguc cuuccgcguc cugggcgaca agagcgagga ccugcagauc      360

aagcagcccu ucuucggcga caaggagcuc gagaccgccu accacaagaa guaccccacc      420

aucuaccacc uccggaagca ccuggccgac gccgacaaga acucccccgu ggcggacauc      480

cgcgaggugu acauggccau cagccacauc cugaaguacc gggggcacuu ccugacccug      540

gacaagauca acccgaacaa caucaacaug cagaacuccu ggaucgacuu caucgagagc      600

ugccaggagg ucuucgaccu cgagaucucc gacgagagca agaacaucgc cgacaucuuc      660

aaguccagcg agaaccgcca ggagaaggug aagaagaucc ugcccuacuu ccagcaggag      720

cugcucaaga aggacaaguc caucuucaag cagcugcugc agcuccuguu cggccugaag      780

acgaaguuca aggacugcuu cgagcucgag gaggagcccg accugaacuu cagcaaggag      840

aacuacgacg agaaccugga gaacuuccuc gggucccugg aggaggacuu cagcgacgug      900

uucgccaagc ugaagguccu ccgggacacc auccugcugu ccggcaugcu caccuacacc      960

ggggcgacgc acgcccgcuu cagcgccacc augguggagc gguacgagga gcaccgcaag     1020

gaccugcagc gguucaaguu cuucaucaag cagaaccuga gcgagcagga cuaccucgac     1080

aucuucggcc gcaagaccca gaacggcuuc gacguggaca aggagaccaa gggguacguc     1140

ggcuacauca cgaacaagau ggugcugacc aacccccaga agcagaagac cauccagcag     1200

aacuucuacg acuacaucuc cgggaagauc accggcaucg agggcgccga guacuuccug     1260

aacaagauca gcgacgggac guuccuccgc aagcugcgga ccuccgacaa cggcgccauc     1320

ccgaaccaga uccacgcgua cgagcuggag aagaucaucg agcgccaggg gaaggacuac     1380

cccuuccucc uggagaacaa ggacaagcug cucagcaucc ugaccuucaa gauccccuac     1440

uacgugggcc cccuggccaa gggguccaac agccgguucg ccuggaucaa gcgcgccacc     1500

uccagcgaca uccucgacga caacgacgag gacacgcgga acggcaagau ccgcccgugg     1560

aacuaccaga agcugaucaa cauggacgag acccgggacg ccuucaucac caaccugauc     1620

ggcaacgaca ucauccuccu gaacgagaag guccugccca agcgcucccu gaucuacgag     1680

gaggugaugc uccagaacga gcugacccgg gugaaguaca aggacaagua cgggaaggcg     1740

cacuucuucg acagcgagcu gcgccagaac aucaucaacg gccucuucaa gaacaacucc     1800

aagcggguca acgccaagag ccugaucaag uaccuguccg acaaccacaa ggaccucaac     1860

gccaucgaga ucgugagcgg ggucgagaag ggcaaguccu ucaacagcac gcugaagacc     1920

uacaacgacc ugaagaccau cuucuccgag gagcuccugg acagcgagau cuaccagaag     1980

gagcuggagg agaucaucaa ggugaucacc guguucgacg acaagaaguc caucaagaac     2040

uaccucacga aguucuucgg gcaccuggag auccuggacg aggagaagau caaccagcuc     2100

agcaagcugc gcuacagcgg cuggggccgg uacuccgcca agcugcuccu ggacauccgc     2160

gacgaggaca ccggguucaa ccugcuccag uuccugcgga acgacgagga gaaccgcaac     2220

cugaccaagc ucaucagcga caacacccug uccuucgagc ccaagaucaa ggacauccag     2280

agcaagucca cgaucgagga cgacaucuuc gacgagauca agaagcuggc cggcagcccc     2340

gcgaucaagc gggggauccu caacuccauc aagaucgucg acgagcuggu gcagaucauc     2400

ggcuacccgc cccacaacau cgugaucgag auggcccgcg agaacaugac caccgaggag     2460

gggcagaaga aggccaagac ccggaagacc aagcuggaga gcgcccucaa gaacaucgag     2520

aacucccugc uggagaacgg caaggucccc cacagcgacg agcagcucca guccgagaag     2580

cuguaccugu acuaccucca gaacggcaag gacauguaca cgcuggacaa gaccgggagc     2640

cccgccccgc uguaccucga ccagcuggac caguacgagg uggaccacau cauccccuac     2700

uccuuccugc ccaucgacag caucgacaac aaggugcuca cccaccgcga gaacaaccag     2760

cagaagcuga acaacauccc cgacaaggag accgucgcga acaugaagcc guucugggag     2820

aagcuguaca acgccaagcu caucucccag acgaaguacc agcgccugac caccagcgag     2880

cggacccccg acggcgugcu gacggagucc augaaggccg gguucaucga gcgccagcuc     2940

guggagaccc ggcagaucau caagcacguc gcccgcaucc uggacaaccg guucagcgac     3000

accaagauca ucacccucaa gagccagcug aucacgaacu uccgcaacac cuuccacauc     3060

gcgaagaucc gggagcugaa cgacuaccac cacgcccacg acgccuaccu ggccguggug     3120

gucggccaga cccuccugaa gguguacccc aagcuggccc ccgagcucau cuacggccac     3180

cacgcgcacu ucaaccgcca cgaggagaac aaggccaccc ugcggaagca ccuguacucc     3240

aacaucaugc gcuucuucaa caacccggac agcaaggugu ccaaggacau cugggacugc     3300

aaccgggacc uccccaucau caaggacguc aucuacaaca gccagaucaa cuucgugaag     3360

cgcacgauga ucaagaaggg ggccuucuac aaccagaacc ccgugggcaa guucaacaag     3420

cagcuggccg ccaacaaccg guacccgcug aagaccaagg cgcucugccu ggacaccucc     3480

aucuacgggg gcuacgggcc caugaacagc gcccugucca ucaucaucau cgccgagcgc     3540

uucaacgaga agaagggcaa gaucgagacc gucaaggagu uccacgacau cuucaucauc     3600

gacuacgaga aguucaacaa caaccccuuc caguuccuca acgacacgag cgagaacggc     3660

uuccugaaga agaacaacau caaccgggug cugggguucu accgcauccc caaguacucc     3720

cucaugcaga agaucgacgg cacccggaug cuguucgaga gcaaguccaa ccugcacaag     3780

gccacccagu ucaagcucac caagacgcag aacgagcugu ucuuccacau gaagcgccug     3840

cucaccaaga gcaaccugau ggaccugaag uccaagagcg ccaucaagga gucccagaac     3900

uucauccuca agcacaagga ggaguucgac aacaucagca accagcuguc cgcguucagc     3960

cagaagaugc uggggaacac caccucccuc aagaaccuga ucaagggcua caacgagcgg     4020

aagaucaagg agaucgacau ccgcgacgag acgaucaagu acuucuacga caacuucauc     4080

aagauguuca gcuucgucaa gagcggggcc ccgaaggaca ucaacgacuu cuucgacaac     4140

aagugcaccg uggcccggau gcgccccaag cccgacaaga agcugcucaa cgccacccug     4200

auccaccagu ccaucaccgg ccuguacgag acgcgcaucg accucagcaa gcugggcgag     4260

gacuga                                                                4266


<210>  2168
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2168
augaagaaga uccugggccu cgaccugggg cccaacagca ucggcugggc ccuggugaac       60

gaggccgaga acgagaacga gaaguccagc aucaucaaga ucgggucccg cgucauccac      120

uacggcgaca accucgugaa gguggacaag agcgggaaga ucuccgcgag caugaccccg      180

gaggaggacu ucuccagcgg caagggccug ucccccaacg ccgggcggac caagcagcgc      240

agcgcccggc gcaaccugca gcgguacaag cagcgccgga acaaccucau cgagauccug      300

aaggcccacu cccugaucga caacaacuuc aucuucagcg aggagggcaa cgagacgacc      360

uucaagaccu acaagaaccg cgcccucgcg gccaccaacg agaucucccu ggaggacuuc      420

aucaaggucc uguucaugau caacaagaag cggggguaca agagcucccg caaggccaag      480

ggcgacgagg acgggcagcu caucgacggc auggccaucg cgauccagcu guacgagcac      540

aaccagacgc ccggccaguu cguguacaac cugcucaaga acgggaagaa gaacaucccc      600

gacuucuacc ggagcgaccu gcagaccgag uucgaccgca ucuggaacuu ccagaaggcc      660

uuccaccagg agucccugag cgacggcauc ugcgagaagc ucguggggca gaagaagaac      720

gucaccuccg ccauccuguu caaggaguuc aagaucgaga gccuggagau caagggcaag      780

ugggacgaga agcgguucuu ccgcuacgag cuccggaaca aggccgugca gcaggagcug      840

aagcugaacg agaucgccga gugccucgug gagaucaacg gcgagaucag caacuccagc      900

ggguaccugg gcgcgaucuc cgaccgcagc aaggagcugg ucuuccagaa gaucaccgug      960

gggcaguacc ucuacaagca gaucgccgcc aacccgaaca cgucccugaa gaagcagauc     1020

uucuaccggc aggacuaccu ggacgaguuc aacacccucu gggagaccca ggccaaguac     1080

cacaaggagc ugaccgacga gcugaagacg cagauccgcg acgugaucau cuucuaccag     1140

cggcgccuca agagccagaa gggccugauc uccuucugcg aguucgagag caagcagauc     1200

gaggucuucg uggacgggaa gaaguccauc aagacccggg gcaugcgcgu gauccccaag     1260

agcagccccc uguuccagga guucaagauc uggcagaucc ucaacaacau cgagaucacc     1320

gaccugaaga ccaaggagag ccgcgagcug acggacgacg agcggcagcu ccuguucgag     1380

gagcugaaca uccgccccaa gaucuccaag aaggaggccc ucaagauccu gaccgagaag     1440

ccggcgcagu acgaccugaa cuacaaggac aucgagggca acaagaccaa cgccaaguuc     1500

uacgaggccu acaacaagau cauccaccuc agcgggcacg acgagaucga cuucaccgac     1560

aagacguccg acaucaacga guaccugaag cagaucuuca gccugcucgg caucaagccc     1620

gagauccugg acuucgacuc cagccugaag gacgaggacu ucgagaagca gcugcacuuc     1680

cagcucuggc accuccugua cuccuucgag ggggacaaca ccaagaccgg cgacgagaag     1740

cugcugcaga agcugaacac caaguucggg uuccccgucg aguacgccaa ggugaucagc     1800

aacaucgccc uccccgacga cuacggcagc cuguccgcga aggccauccg gaagauccug     1860

ccgcaccuca aggacggcuu cagcuacggg ggccgcaagu cccggcccga cgagcccagc     1920

gccugcgagu acgccgggua ccgccacucc aaggccagcc ugacgaagga cgagaaggag     1980

aacaagaccu acaaggacaa gcuggagauc cuccccaaga acucccugcg gaacccgauc     2040

guggagaaga uccugaacca gaccaucaac gucgugaacg cggugaucgg cgacuacggg     2100

aagcccgacg agauccgccu cgagcuggcc cgggagcuga agaaguccgc cgaggagcgc     2160

caggacgcca ccgacuccau cagcaaggcg acggccaagc acgagaagua ccggaagguc     2220

cuccagaccg aguucggccu gaagcacauc ucccgcaacg accugauccg guacaagcuc     2280

uaccuggagc ugaagaacaa cggcuucaag acccucuaca gcgacaccua caucgccccc     2340

uacgagaucu ucuccaagaa guucgacauc gagcacaucc ugcccaaggc caagcuguuc     2400

gacgacagcu ucuccaacaa gacgcucgag cucaagagcg ugaaccugaa gaagggggac     2460

augaccgccu acgacuucgu gaaggaggag uacggcaccg aggggcuggc gaaguacgag     2520

cagcgcgucc aggaccuguu ccugaacaag aagcggaacg aggaccucga ccaggagaac     2580

gagucccgca ucaccgacga gaagaucagc aagacgaagc gggacaagcu gcugaugccg     2640

gagaacaaga uccccaccgg cuucaucgac cgcgagcucc gggacagcca guacaucgcc     2700

aagaaggcca agaccaugau ggaggacauc ugcaaggacg uggucaccac cacgggcucc     2760

gugaccgacc gccugcgcga ggacuggcag cugaucgaca ucaugcagga gcucaacugg     2820

cccaaguacg ccgcccuggg gcugaccgag gaguucacca acgaggaggg caacgugauc     2880

cggaagauca aggacuggac gaagcgcaac gaccaccggc accacgcgau ggacgccauc     2940

accaucgccu ucaccaagcg cagccacauc caguaccuca acaaccuguc cgcccggagc     3000

gacaagucca gcuccaucua cgggaucgag cagaaggagc uguaccgcga caagaacaac     3060

aagcuccggu ucgcgccccc gauccccauc aacgaguucc gcgccgaggc caagaugcag     3120

cuggagaaca cccugaucag caucaaggcc cagaacaagg ucgugacgaa gaacaagaac     3180

aagaucuccg gccagaagaa gcuccaggag acccugacgc cccgggggca gcugcaccuc     3240

gagaccaucu acggcaagau caaccaguac cagaccgccg agaucaaggu gggcgggacc     3300

cuggacagcg agcugaucaa caaggucgcg aacaagaaga cccgcgaggc ccuccugaac     3360

cggcuggccg aguucggcaa cgaccccaag aaggccuuca ccgugaagaa cacgaucgac     3420

aagaacccga ucuaccucga caagcaccag uccuacaaga uccccgagaa ggugaagauc     3480

gucuggcagg agcccgugua caccauccgc aagagcauca ccccggaccu gaagcuggac     3540

aagaucaucg accccaagau caaggcccuc cuggaguccc ggcugaagga gugcaacggg     3600

gacgcgaagc ucgccuucag caaccuggcc gagaacccca ucuggcugaa caaggagaag     3660

ggcauccaga ucaagcgcgu gaccaucacg gggaucucca acgccgccag ccuccacgac     3720

aagaaggacc ugaacggcaa gcccauccug gacgagaagg gcaacaagau cuccgucgac     3780

uucgugaaca ccgggaacaa ccaccacgug gcgaucuaca aggacgagaa cggcaaccuc     3840

cacgacaacg ucgugaccuu cuucgaggcc guggagcggg ucaaccagaa ccugccggug     3900

guggacaagc uguucaagca gaaggagggg uggcaguucc ucuucaccau gaagcagaac     3960

gaguacuucg ucuuccccaa cgagaucacg gccuucaacc ccaacgaguu cgaccugcug     4020

aaccccgaca acuacuaccu caucagcccg aaccuguucc gcgugcagac cauguccaag     4080

gugauguacg gcaacaacac ccugcgggac uacaaguucc gccaccaccu cgagaccacg     4140

cugcaggaca ucaaggagcu gcgggacauc gucuucaagc aguucaagag ccucagcauc     4200

uucgacaaga ucgugaaggu gcgcaucaac caccugggga agaucgucaa gaccggcgag     4260

uacuga                                                                4266


<210>  2169
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2169
augaaggaca uccugggccu cgaccugggg accaacagca ucggcugggc cguggucaag       60

gaggcccaga acgacaacga gcaguccgag aucguggacg uggggguccg cgugaacccc      120

cugaccacgg acgagaagac caacuucgag aagggcaagc cgaucagcac caacgcggac      180

cggacccuca agcgcuccgc ccggcgcaac cugcagcggu acaagcugcg ccggaaggag      240

cucaucgagc ugcugaagga gcaccagcuc aucagcacgg acaccauccu gaccgagaac      300

gggaagggca ccacguucca gacccuggag cuccgcgcca aguccgccaa ggagaagauc      360

agccuggagg agcuggcccg gguguuccuc accaucaaca agaagcgcgg cuacaagucc      420

agccggaagg cgaagaacga ggacgagggg cagcugaucg acggcaugga gaucgccaag      480

cgccuguacg aggagaaccu caccacgggg cagcuguccc ugcagcuccu gaaggagggc      540

aagaaguccc ugcccgacua cuaccggucc gaccuccagu ucgagcugga caaggucugg      600

aacuuccaga agcaguucca cagccacauc cugaccgacg aguucuacgc ccagcucaag      660

gggaagggcc agcgcgccac cuccgcgcug uuccuggcca aguaccagau cuacaccgcc      720

gagaacaagg gcagccggga ggacaagaag cuccaggccu accaguggcg cguggacgcc      780

cuguccaagg agcugacgcg ggagcagcuc gcguucguga ucaccgagau caacaacaac      840

cugaacaaca gcuccgggua ccugggcgcc aucagcgacc gcuccaagga gcucuacuuc      900

aacaacgaga ccgucgggga guaccugugg aagcagaucg ccgccaaccc ccacaccagc      960

cugaagaacc agguguucua ccggcaggac uaccucgacg aguucgagca gaucuggaag     1020

acgcagagcg ccuucuaccc cgagauccug accgaguccc ugaagaagga gcuccgcgac     1080

guggucaucu ucuaccagcg gaagcucaag agccagaagu cccuggugag cuucugcgag     1140

uucgagaaca aggagaucga gaucaagaag gaggacggca ccuccaccaa gaaggugauc     1200

ggccucaagg ucgcgcccaa gagcagcccc cuguuccagg aguucaagau cuggcagaac     1260

cugaacaacg ugcucauccg caagaagggg agcaagacga agaaggccgc caagaaccag     1320

caggccgugc uguucgagga ggagaagacc aucuccgagc uggaccugga ggccaagcag     1380

gcgcuguuca acgagcugaa ccugaagggc aaccucaagg ccgacuacug ccuggagauc     1440

cugggguaca gccccaagga gugggagaug aacuacuccg uccucgaggg caaccgcacc     1500

aacaaggccc uguacgaggc cuaccugaag aucaccgacc ucgaggggua cgacgccaag     1560

aaccaccuga aggugaagcu gaacaaggac gagguggagc ucgacgaccu gaaggucccc     1620

gcgagcgaga ucaaggacau ggugcaggcc aucuucaagg aggccggcau caacacggac     1680

auccugcacu ucaacgccga gcucgacggc aaggacuucg agaaccaggc guccuaccag     1740

cuguggcacc uccuguacag cuacgagggg gacgacucca agagcggcaa cgagcugcug     1800

uacaagcucc uggagaccaa guucggguuc aagaaggagu acgcccagau ccuggccaac     1860

gugucccuca gcgacgacua cggcucccug agcaccaagg ccaugcggaa gauccacccg     1920

uacaucaagg agaacaaguu cagcaccgcc ugcgagcugg cgggguaccg ccacuccaag     1980

cacagccuca cgaaggagga gaacgacaac cggguccugc gcgacucccu ggaggugcuc     2040

aagaagaaca gccugcggca gcccgucgug gagaagaucc ugaaccagau gaucaaccuc     2100

gugaacgccc ugcugaagaa guaccgcacc gagaacccca acuucaaguu cgacgagauc     2160

cggaucgagc ucgcccgcga gcugaagaag aacgccgagg agcgggccaa caugaccgcg     2220

aacaucaaca aggccaagac cgagcacgag aagaucauca agauccugaa gaacgaguuc     2280

ggccucccca acccgacgcg caacgacauc auccgguacc gccuguacga ggagcugaag     2340

gccaacggcu acaaggaccu cuacaccaac aucaagaucc cccaggagaa gcuguucacc     2400

aaggagaucg acaucgagca caucaucccc aagucccggg ucuucgacga cagcuucucc     2460

aacaagaccc ugagcuuccg caagaccaac cucgacaagg gggagcggac ggccuucgac     2520

uacauggagu ccaaguucgg cgccgacaag cuggaggagu acguggcgcg cguggaggac     2580

cuguacaaga acaacaucau cagcaaggcc aaguaccaga agcuccugaa gaaggagucc     2640

gagaucgggg acggcuucgu cgagcgggac cugcgcgaga cccaguacau cgccaagaag     2700

gccaaggaga uccuccugca gaucaccccc aacgugcuga gcaccuccgg gggcaucacg     2760

gaccggcucc gcgaggacug ggaccugauc aacgugauga aggagcugaa ccucccgaag     2820

uaccgcgcgc ugggccugac cgagauggag gaccggaagu acgggcagca ggucgaggug     2880

aucaucgacu ggaccaagcg caacgaccac cggcaccacg ccauggacgc ccucaccgug     2940

gccuucacga agcacaacca cauccaguac cugaacuacc ugaacgcccg caagaacgag     3000

aagcacaagg agcacagcaa caucaugggc auccagcagc ucgagaccau caaggucacc     3060

gacaagaacg ggaacgagaa gcggguguuc aaggcgccca ugcccaacuu ccgccaggug     3120

accaaggccu uccuggagaa cauccugauc ucccacaagg ccaagaacaa ggucgugacg     3180

aagaacaaga acaagguggc cggcagcaac aagguccagg aggugcucac cccgcggggc     3240

cagcugcaca aggagaccgu guacgggaag uaccaguacu acguccagaa ggaggagaag     3300

aucggcggga aguucgaccu ggagaccauc aagaaggugg ccaacccgcu cuaccagucc     3360

cugcugaaga agcgccucga ggagaacggc ggggacccca agaaggcguu cacgggcaag     3420

aacagccugg ccaagaaccc caucuaccug gacgacgccc agaccgagca gcuccccgag     3480

aaggugaagc uggucuggcu ggagaacagc uucuccaucc ggaaggacau caccccggag     3540

aacuucaagg acgagaagcu caucgacaag auccuggacg agggcgugaa gcgcauccug     3600

aaggagcggc uccagagcuu cgggaacgac gccaagaagg ccuucuccga ccuggagaag     3660

aaccccaucu ggcugaacaa ggagaagggc aucaccauca agcgcgugac gaucagcggg     3720

gucgcgaacg ccgagccccu ccacaccaag aaggaccacc ugggcaagga gauccuggac     3780

aaggagggga agcccauccc gguggacuuc aucuccaccg gcagcaacca ccacguggcc     3840

gucuaccggg acgagaaggg caaccuccag gagaaggugg uguccgucuu cgaggccgug     3900

gugcgcgcga accaggggcu gcccaucauc gacaagaccu acaaccagca ccugggcugg     3960

gaguuccucu ucacgaugaa gcagaacgag auguucgucu uccccaacga gagcaccggg     4020

uucaacccga acgacaucga ccugcuggac cccaagaaca agcggaucau cucccccaac     4080

cucuuccgcg ugcagaagcu ggccaccggc gacuacuggu uccggcacca ccuggagacc     4140

acgguggaga ccaagagcgc caccaaggac augaccuaca agcgccuguc ccucaaccac     4200

aucaagaaca ucaucaaggu ccggaucagc caccuggggg acaucguguc cgugggcgag     4260

uacuga                                                                4266


<210>  2170
<211>  4275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2170
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga ccgacgagga ggucuccagc aucauccggc ucggggugcg cgugaacccc      120

cugacggucg acgagaccca gaacuucgag aagggcaagu ccaucaccac caacgcggag      180

cggacgcuga agcgcagcau gcggcgcaac cuccagcggu acaagcugcg ccgggacgcc      240

cugguggagu uccucaagga gcugggguuc aucaccgacg agaccauccu guccgagcag      300

ggcaaccgca ccacguucga gaccugccgg cuccgcgcca aggccgccga ggaggagauc      360

agccuggagg agcugucccg ggugcuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ucaagaagga ggaggacggg gugcugaucg acggcaugga gaucgcgaag      480

aagcucuacg aggaggaccu gaccccgggg cagcugugca gccagcuccu ggaguccggc      540

aagaaggugc ugcccgacuu cuaccgcagc gaccuccagg aggagcugga ccggaucugg      600

uccguccaga agcaguucca ccccgacgug uucugcgacg ccgccaagga ggagaucaag      660

gggaagaacc gcagccagac cugggccauc cuggcgaacu acuucgugug gaaggaggag      720

gucgugggcu ggaacgaccg ggaggccaag aacgagacga ucgagaagga gugcaagcuc      780

gugggccuga agcgcaccac caagggguac gagcugaaga aggagaacua ccgguggcgc      840

gcccaggccc ucaccgagca gcugggccug gaggagaucg ccgucgugcu ccaggagauc      900

aacgggcaga ucaacgcguc cagcggcuac cugggcgcca ucuccgaccg cagcaaggug      960

cuguacuuca accaccagac ggucgggcag uaccagaugg ccgagcucga caagaacccc     1020

cacgccuccc ugcggaacau gguguucuac cgccaggacu accuggacga guucgaccgg     1080

aucugggaga agcaggccga guuccacaag gagcucaccg cggagcugaa gaaggagauc     1140

cgcgacauga ucaucuucua ccagcggcgc cucaagagcc agaagggccu caucagcuuc     1200

ugcgaguucg agcggcugga gaucgugguc gagaaggacg ggaagaagca gaccaaggug     1260

aucggcugca aggugauccc gcgcucccac ccccuguucc aggaguucaa ggucuggcag     1320

acccucaacg acaucaaggu gcuggggcgg gagaagaagc gcaacgcccu gagcgccucc     1380

gagagccggg cccucuaccc cgaggagaag gagauccugg cccgcgagcu ggcgaucaag     1440

gagaacauga agaaggccga cguccugaag cuccuguucg agaaccccca ggagcuggac     1500

cucaacuuca agcagaucga cggcaaccgg acgggcuucu cccuguucag cgccuacucc     1560

aagaugaucg agaaguacgg guacgagccg cuggacuuca agaagcccgc cgacgagauc     1620

aucggccagc uccgcaccau cuucaccgac cuggggugga acaccaaccu guucacgauc     1680

gaccucacca aggagggcaa ggagcuggag augcagcccu acuuccggcu guggcaccuc     1740

cuguacagcu ucgaggggga caacaccucc accggcaacg gcaagcugau cgagaagauc     1800

augcagcucu gcggggugga gaaggaguac gccguggagc uggcgagccu guccuuccag     1860

gacgacuacg gcagccucuc cgccaaggcc aucaagcgca uccugcccua ccugaaggag     1920

gggaaccucu acgacgucgc cugcgaguac gcgggcuacc ggcacagcaa guccagccug     1980

acgaaggagg agaucgagaa caaggugcug aaggggaagc ucgaggugcu gccgaagaac     2040

ucccugcgca accccgucgu ggagaagauc cucagccaga uggugaacgu caucaacacc     2100

aucaucgaca ccuacggcaa gcccgacgag auccgggugg agcuggcccg cgagcugaag     2160

aaguccgcca aggagcgcga ggagcucacc aaggccaucg ccaagagcac gcgggagcac     2220

gaggagaucc gcaagcugcu gcaggacgag uucggcauga ugaacgugag ccggaacgac     2280

aucauccgcu acaagcucua cgaggagcug aaggacaacg gguacaagac ccuguacucc     2340

aaccaguaca uccccaagga gaagaucuuc agcaaggaga ucgacaucga gcacaucauc     2400

ccgcaguccc ggcuguucga cgacagccug uccaacaaga cccuggagua caaggcgauc     2460

aacaucgaga agggcaacaa gaccgccuac gacuucguca aggagaagua cggggaggag     2520

ggccuccagc aguacaucaa ccgcugcgag agccuguuca acgacaagaa ggccaagcug     2580

cggaagcuca agauggagca gaaggacauc cccgacggcu ucaucgaccg cgaccugcgg     2640

aacacgcagu acaucgccaa gaaggcccug gcgaugcuca acgagaucug ccgccgggug     2700

guggccacca ccggguccau caccgaccag cugcgcgagg acuggcagcu ggucgacgug     2760

augaaggagc ucaaccugcc caaguacgag gcccugggcu ucguggagac cuacgaggac     2820

aaggacgggc ggaagaucaa gcgcaucaag gacuggacga agcggaacga ccaccgccac     2880

cacgccaugg acgcccucac cgucgcguuc accaaggacg ccuucaucca guacuucaac     2940

aacaagaacg ccgcccagga cagcggcaac cggaaccaca ccaacaucau cgggaucaag     3000

acgcgcuacu ucgacaaggg ccgggcgcug cccccgaucc cccuggagca guuccgcauc     3060

gaggccaagc agcaccucga gaagcugcug guguccauca aggccaagaa caaggugguc     3120

accgugaacg ugaaccggac caagaagcgc aagggcgaga acaccaagau ccagcagacg     3180

ccccgcgggc agcuccaccu ggagaccguc uacggcagcc acaagcagua cgugaccaag     3240

aucgagaagg ugaacgccuc cuucgacgcc gcgaagaucg ccaccgucag caagcgggcc     3300

uaccgcaacg cccugcucaa gcggcuggag acguucggga acgaccccaa gaaggccuuc     3360

accggcaaga acgcgcugga gaagaacccg cucuacgccg acaaguacca gaccauccgc     3420

gugcccgaga aggugcagac cgucgaguuc gagacgaucu acaccauccg gaagcccgug     3480

gaccccgccc ugaacgugga caaggucgug gacgugaagg uccgcgccau ccuggagcgg     3540

cgccucaagg aguacggggg cgacccgaag aaggccuucg ugaacuucug ggagaacccc     3600

aucuggcuga acaaggagaa gggcaucucc aucaagcggg ugagcauccg cgggaucaac     3660

aacgcgcagu ccauccacgu caagaaggac aagaacggca accccaucug ggacgagaac     3720

gggaagcaga ucccggugga cuucgugaac accggcaaca accaccacgu cgccaucuac     3780

cggaagcccg ugcuggacaa gaagggccag gugaccuucg acgaggacgg gaaccucaug     3840

uacgagcugg acgaggucgu ggugcccuuc uucgaggccg ucacgcgcgc caaccugggc     3900

cuccccauca ucgacaagga cuaccgcaag agcgaggggu ggcaguuccu guucuccaug     3960

aagcagaacg aguacuucgu guucccgaac gagaagaccg gcuucaaccc caaggaggug     4020

gaccugcuca accccgacaa cuacgcgaug aucagcccca accuguuccg gguccagacc     4080

augagcaagg ugauguacgg gaacaacguc gugcgggacu acaaguuccg ccaccaccug     4140

gagaccacgg ugaaggacau gaaggagcuc aaggacaucg ccuacaagca guacaagacc     4200

cuguccuucg gcaacagcgu cgugaagauc cggaucaacc acaucgggca gaucgugucc     4260

gucggcgagu acuga                                                      4275


<210>  2171
<211>  4275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2171
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga ccgacgagga ggucuccagc aucauccggc ucggggugcg cgugaacccc      120

cugacggucg acgagaccca gaacuucgag aagggcaagu ccaucaccac caacgcggag      180

cggacgcuga agcgcagcau gcggcgcaac cuccagcggu acaagcugcg ccgggacgcc      240

cugguggagu uccucaagga gcugggguuc aucaccgacg agaccauccu guccgagcag      300

ggcaaccgca ccacguucga gaccugccgg cuccgcgcca aggccgccga ggaggagauc      360

agccuggagg agcugucccg ggugcuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ucaagaagga ggaggacggg gugcugaucg acggcaugga gaucgcgaag      480

aagcucuacg aggaggaccu gaccccgggg cagcugugca gccagcuccu ggaguccggc      540

aagaaggugc ugcccgacuu cuaccacagc gaccuccagg aggagcugga ccgcaucugg      600

uccguccaga agcaguucca ccccgacgug uucugcgacg ccgccaagga ggagaucaag      660

gggaagaacc ggagccagac cugggccauc cuggcgaacu acuucgugug gaaggaggag      720

gucgugggcu ggaacgaccg cgaggccaag aacgagacga ucgagaagga gugcaagcuc      780

gugggccuga agcggaccac caagggguac gagcugaaga aggagaacua ccgcuggcgg      840

gcccaggccc ucaccgagca gcugggccug gaggagaucg ccgucgugcu ccaggagauc      900

aacgggcaga ucaacgcguc cagcggcuac cugggcgcca ucuccgaccg cagcaaggug      960

cuguacuuca accaccagac ggucgggcag uaccagaugg ccgagcucga caagaacccc     1020

cacgccuccc ugcgcaacau gguguucuac cggcaggacu accuggacga guucgaccgc     1080

aucugggaga agcaggccga guuccacaag gagcucaccg cggagcugaa gaaggagauc     1140

cgggacauga ucaucuucua ccagcgccgg cucaagagcc agaagggccu gaucagcuuc     1200

ugcgaguucg agcgccugga gaucgugguc gagaaggacg ggaagaagca gaccaaggug     1260

aucggcugca aggugauccc gcggucccac ccccuguucc aggaguucaa ggucuggcag     1320

acccucaacg acaucaaggu gcuggggcgc gagaagaagc ggaacgcccu gagcgccucc     1380

gagagccgcg cccucuaccc cgaggagaag gagauccugg cccgggagcu ggcgaucaag     1440

gagaacauga agaaggccga cguccugaag cuccuguucg agaaccccca ggagcuggac     1500

cucaacuuca agcagaucga cggcaaccgc acgggcuucu cccuguucag cgccuacucc     1560

aagaugaucg agaaguacgg guacgagccg cuggacuuca agaagcccgc cgacgagauc     1620

aucggccagc uccggaccau cuucaccgac cuggggugga acaccaaccu guucacgauc     1680

gaccucacca aggagggcaa ggagcuggag augcagcccu acuuccgccu guggcaccuc     1740

cuguacagcu ucgaggggga caacaccucc accggcaacg gcaagcugau cgagaagauc     1800

augcagcucu gcggggugga gaaggaguac gccguggagc uggcgagccu guccuuccag     1860

gacgacuacg gcagccucuc cgccaaggcc aucaagcgga uccugcccua ccugaaggag     1920

gggaaccucu acgacgucgc cugcgaguac gcgggcuacc gccacagcaa guccagccug     1980

acgaaggagg agaucgagaa caaggugcug aaggggaagc ucgaggugcu gccgaagaac     2040

ucccugcgga accccgucgu ggagaagauc cucagccaga uggugaacgu caucaacacc     2100

aucaucgaca ccuacggcaa gcccgacgag auccgcgugg agcuggcccg ggagcugaag     2160

aaguccgcca aggagcgcga ggagcucacc aaggccaucg ccaagagcac gcgcgagcac     2220

gaggagaucc ggaagcugcu gcaggacgag uucggcauga ugaacgugag ccgcaacgac     2280

aucauccggu acaagcucua cgaggagcug aaggacaacg gguacaagac ccuguacucc     2340

aaccaguaca uccccaagga gaagaucuuc agcaaggaga ucgacaucga gcacaucauc     2400

ccgcaguccc gccucuucga cgacagccug uccaacaaga cccuggagua caaggcgauc     2460

aacaucgaga agggcaacaa gaccgccuac gacuucguca aggagaagua cggggaggag     2520

ggccuccagc aguacaucaa ccggugcgag agccuguuca acgacaagaa ggccaagcug     2580

cgcaagcuca agauggagca gaaggacauc cccgacggcu ucaucgaccg ggaccugcgc     2640

aacacgcagu acaucgccaa gaaggcccug gcgaugcuca acgagaucug ccggcgcgug     2700

guggccacca ccggguccau caccgaccag cugcgggagg acuggcagcu ggucgacgug     2760

augaaggagc ucaaccugcc caaguacgag gcccugggcu ucguggagac cuacgaggac     2820

aaggacgggc gcaagaucaa gcggaucaag gacuggacga agcgcaacga ccaccggcac     2880

cacgccaugg acgcccucac cgucgcguuc accaaggacg ccuucaucca guacuucaac     2940

aacaagaacg ccgcccagga cagcggcaac cgcaaccaca ccaacaucau cgggaucaag     3000

acgcgguacu ucgacaaggg ccgcgcgcug cccccgaucc cccuggagca guuccggauc     3060

gaggccaagc agcaccucga gaagcugcug guguccauca aggccaagaa caaggugguc     3120

accgugaacg ugaaccgcac caagaagcgg aagggcgaga acaccaagau ccagcagacg     3180

ccccgcgggc agcuccaccu ggagaccguc uacggcagcc acaagcagua cgugaccaag     3240

aucgagaagg ugaacgccuc cuucgacgcc gcgaagaucg ccaccgucag caagcgcgcc     3300

uaccggaacg cccugcucaa gcgccuggag acguucggga acgaccccaa gaaggccuuc     3360

accggcaaga acgcgcugga gaagaacccg cucuacgccg acaaguacca gaccauccgg     3420

gugcccgaga aggugcagac cgucgaguuc gagacgaucu acaccauccg caagcccgug     3480

gaccccgccc ugaacgugga caaggucgug gacgugaagg uccgggccau ccuggagcgc     3540

cggcucaagg aguacggggg cgacccgaag aaggccuucg ugaacuucug ggagaacccc     3600

aucuggcuga acaaggagaa gggcaucucc aucaagcgcg ugagcauccg ggggaucaac     3660

aacgcgcagu ccauccacgu caagaaggac aagaacggca accccaucug ggacgagaac     3720

gggaagcaga ucccggugga cuucgugaac accggcaaca accaccacgu cgccaucuac     3780

cgcaagcccg ugcuggacaa gaagggccag gugaccuucg acgaggacgg gaaccucaug     3840

uacgagcugg acgaggucgu ggugcccuuc uucgaggccg ucacgcgggc caaccugggc     3900

cuccccauca ucgacaagga cuaccgcaag agcgaggggu ggcaguuccu guucuccaug     3960

aagcagaacg aguacuucgu guucccgaac gagaagaccg gcuucaaccc caaggaggug     4020

gaccugcuca accccgacaa cuacgcgaug aucagcccca accuguuccg gguccagacc     4080

augagcaagg ugauguacgg gaacaacguc gugcgcgacu acaaguuccg gcaccaccug     4140

gagaccacgg ugaaggacau gaaggagcuc aaggacaucg ccuacaagca guacaagacc     4200

cuguccuucg gcaacagcgu cgugaagauc cgcaucaacc acaucgggca gaucgugucc     4260

gucggcgagu acuga                                                      4275


<210>  2172
<211>  4278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2172
augcgccgga acauccuggg ccucgaccug gggaccacca gcaucggcuu cgccaaggug       60

aucgaggacg aggacuacca gaacuccgcc gucgagcuga ucggggugcg cgugaacccc      120

cucacgaccg acgagcagac caacuucgag aagggccggc cggucagcgu gaacgcggac      180

cgcacccuga agcgggggau gcgccggaac cuggaccgcu accagcuccg gcgccaccac      240

cugaucgagg ugcugaaggc caaccacauc aucacggucg acaccaagcu cgccgaggac      300

ggcaagaaca ccacccacgc cacguacgcc gugcgggcgc uggccgccac cgccgagauc      360

ccccuggagg cguucgcccg cgugcuccug gccaucaaca agaagcgggg cuacaagucc      420

agccgcaagg ccaagaacga ggacgagggg cagcugaucg acggcauggc cgucgcgaag      480

cggcucuacg aggagaaccu gacccccggg cagcuguccu accuccugcu gaaggagggc      540

aagaaguacc uccccgacuu cuaccgcagc gaccugaacg ccgagcugga caaggugugg      600

uccuuccaga agcaguucua cccggagauc cucaccgagg aguucaagaa gcaccuggag      660

gggaagggcc agcgggccac gagcgccgcc uucuggggcc aguacggguu caacaccgcg      720

gagaacaagg gcucccgcga ggagaagaag cugaaggccu accaguggcg gagcgaggcc      780

guggaccagc agcucaccaa ggaggagguc gccuacguga ucaccgagau caacaacgac      840

cugaacaagu ccagcgggua ccugggcgcc aucuccgacc gcagcaagga gcuguucuuc      900

aacaagcaga cggugggcca guaccuguac cagcagcuga agcagaaccc ccacacccgg      960

cucaaggggc aggucuucua ccgccaggac uaccuggacg aguucgagac caucugggag     1020

acccaggcga aguuccaccc cgagcugacg gccgcccuca aggaggagau ccgcgacgug     1080

aucaucuucu accagcggaa gcugaagucc cagaagggcc ugaucagcuu cugcgaguuc     1140

gaguccaagg agaucgaggu ggagaagaac gggcucaaga ucaagaagcg caucggccug     1200

cgggucgccc ccaagagcuc cccgcuguuc caggaguuca agaucuggca gguccucaac     1260

aaccuggagu uccgcaacaa gagcagcaag gagguguucg ugcccgacau gaccgccaag     1320

cagcagcugu ucgacgagcu caacaucaag gggaaccugc uggcguccga ggccaucaag     1380

auccucggcu acaagaccaa ggacugggag gccaacuacc acacccugga gggcaaccgg     1440

acgaacaaga gccuguacga cgccuaccug aagauccucg agcuggaggg guacgacgag     1500

gaccugcuca agcuguccgg cagcgacgac aucgacgugu ccaagaucga gaaguccgcg     1560

agcgagauca agcagauggu gcacgccguc uucgaggugc uggggaucaa cagcaccauc     1620

cucgacuucg acgccgaggc cgacggcaag gccuucgagc agcagaccgc guaccgccug     1680

uggcaccucc ucuacuccgc cgaggacgac aacaagaagu accugcagga ggacaucuuc     1740

aucuacgggc acgacaacau cggccugaag aagaagcucu gcgagcgguu cggcuuccgc     1800

cccgagcaca ccaagauccu ggucaacgug gccuucagcg gggacuacgg cucccugagc     1860

uccaaggcca ugcggaagau cuaccccuuc aucaaggagc ucacguacga caaggccugc     1920

cgcgaggcgg gguaccggca cagcgccucc agccugucca aggaggagau cgccacccgc     1980

ccgcugcaga agcggcucgg ccugcugaag aagaacgagc uccgcagccc cguggucgag     2040

aagauccuga accagauggu gaacgugguc aacgcccuga ucgacgacga gaacaagcgg     2100

cgcaaggccc gggggcucuc cgacgacuuc cacuucgacg agauccgcau cgagcuggcg     2160

cgggagcuga agaagaacgc caaggagcgc gccgagauga ccaccaacau caacgccgcg     2220

aagcagcucc acgagaagau caucaagauc cugcagacgc aguucggcgu gagcaacccc     2280

agccgcaacg acaucauccg guacaagcug uacgaggagc ucaagaacaa cggcuacaag     2340

gaccuguaca ccaacaccua caucuccccc gacauccugu ucagcaagca gaucgacauc     2400

gaccacauca ucccgcagau gucccucuuc gacgacagcu ucuccaacaa gaccgugguc     2460

uuccgcaagg acaaccagga caaggggaac aagacggccu acgacuacau ccugggcaag     2520

uacggggagc uggcccucga ggaguacauc agccggauca ccucccuguu cgagcugggc     2580

aagaagaaca aggaggaggg caucaacaag gccaaguucc agaagcucca gaagcgccug     2640

aucgacaucg gggacggcuu caucgagaag gaccugcggg agagccagua caucgccaag     2700

aaggcgaaga acaugcucua cgccaucugc cgcuccgugg uggccaccag cggggagauc     2760

accgcccggc ugcgcgagga cugggaccug gucaacguga ugcaggagau caaccucccc     2820

aaguuccggg cccugggccu gaccgagaug guggagaaga aggacggggg ccagaaggag     2880

cgcaucaucg acuggacgaa gcggaacgac caccgccacc acgcgaugga cgcccucacc     2940

aucgccuuca cccggcacuc ccacauccag uaccugaacc accugaacgc ccgcaaggac     3000

gaggccagca aguggcaccc cgucgugucc aagaucgagg agaaggagac cgaccucgug     3060

uacgacgacc ugggcaaccg gaagcgccgg uucaaggcgc ccaugccgaa cuuccgcgag     3120

gucgugaagg agcaccugga gaacgugcuc aucagccaca aggccaagaa caaggucgug     3180

acgaagaaca agaacaagau caagaccgcc aagggggaga agaucaagac cgagcugacg     3240

ccccggggcc agcugcacaa ggagaccauc uacgggcgcu accgguacua cgaguccaag     3300

gaggagaagg ugggccccaa guucgacgcc gagaccaucg ggauggucgu gaacccccag     3360

uaccgcaagc uccugcugga gcgccucacc accaacggca acgacccgaa gaaggcguuc     3420

ggcgggaaga acacgcugag caagaacccc cuguacaucg acgaggccaa gcaggagcuc     3480

cugcccgaga agauccugcu ccgguggcug aacgacgacu acuccauccg caaggacgug     3540

acccccgaga accugaagga cgucaagacc aucgagaagg ugcucgacga gggcaucaag     3600

aagauccugc ugaagcggcu cguggacuuc gggaacgacc cgaagaaggc cuucagcgac     3660

cuggacaaga accccaucug gcugaacgag gccaagggca ucaccaucaa gcgcgucacg     3720

aucagcgggg ugaagaacgc cgaggcgcuc cacuacaaga aggaccacuu cggcaacgcc     3780

auccugaacg agaacggcaa gcugaucccc uccagcuucg uguccaccgg gaacaaccac     3840

cacgucgcca ucuaccggga cgccgagggc aaccuccagg agcgcguggu gagccuguuc     3900

gacgccgucc agcuggugaa cgcgggggag gccgugaucg accgggagua caacaagggc     3960

aucggguggc aguuccucuu caccaugaag cagaacgaga uguucgucuu cccgaacgag     4020

aagaccggcu ucgaccccaa ggagaucgac cugcugaaca cggacaaccg caagcucauc     4080

ucccccaacc uguuccgggu gcagaagauc gccagcaaga acuacuucuu ccgccaccac     4140

cuggagacca acguggagga gcggaaggag cucaaggaca ucgucuacaa gccccagcug     4200

ggccugaacg ccaucaagga cguggugaag guccgcauca accaccucgg gaagaucauc     4260

uccgugggcg aguacuga                                                   4278


<210>  2173
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2173
augccccgca agaacauccu gggccucgac cuggggacca ccagcaucgg cuucgccaag       60

aucaucgagg acgacgacua ccagugcucc agcguggagc ugaucggggu ccgggugaac      120

ccgcucacga ccgacgagca gaccaacuuc gagaagggca agcccguguc cgucaacgcc      180

gaccgcaccc ugaagcgggg gaugcgccgg aaccuggacc gcuaccagcu ccggcgcgac      240

uaccugaucg agauccugcg gacgcaccac aucaucaccg cggacaccaa gcucgccgag      300

gacggcaaga acaccacgca caccaccuac gcccugcgcg cccuggccgc gaccgccgag      360

aucccccucg aggccuucgu gcgggugcug cuggccauca acaagaagcg cggcuacaag      420

agcucccgga aggcgaagaa cgaggacgag gggcagcuca ucgacggcau ggccaucgcc      480

cgccggcugu acgaggagag ccugacgccc gggcaguucu ccuaccagcu ccugaaggag      540

ggcaagaagu accugccgga cuucuaccgc agcgaccuca acaccgagcu gaacaagguc      600

uggaacuucc agaagcaguu cuaccccgag auccugaccg acgaguucaa gcggcaccuc      660

gaggggaagg gccagcgcgc caccgccgug gcguucuggg gccagcacgg guucuccaug      720

gccgagaaca agggcagccg ggaggagaag aagcugcacg ccuacaagug gcgcuccgag      780

gccgcccaca agcagcugac gaaggaggag guggcguucg ucaucaccga gaucaacaac      840

gaccucaaca agagcuccgg guaccugggc gccaucagcg accgguccaa ggagcuguuc      900

uucaacaagc agaccguggg ccaguaccuc caccagcagc ugaaggagaa cccccacacc      960

cgccugaagg gccagguguu cuaccgccag gacuaccucg acgaguucga gacgaucugg     1020

gagaugcagg ccaaguucua cagcgcccug acccuggagc ucaaggagga gguccgggac     1080

guggugaucu ucuaccagcg caagcugaag ucccagaugg ggcugaucag ccucugcgag     1140

uucgagucca aggagaucga gaucgagaag aacggggaga aggccaagaa gcggaucggc     1200

cugcgcgucg cgcccaagag cagcccgcug uuccaggagu ucaagaucug gcagguccuc     1260

aacaaccugg aguuccggaa caagaagacc aaggaggugu ucgugcccga gauggccgcc     1320

aagcagcagc uguucgacga gcucaacauc aaggggaacc ugcuggccuc cgaggccauc     1380

aagauccucg gcuacaagac caaggacugg gaggcgaacu accacacgcu ggagggcaac     1440

cgcaccaaca agagccugua cgacgccugg cugaagaucc ucgagcugga gggguacgac     1500

gagaaccugc ucaagcuguc cggcagcgac gacaucgacg uguccaagau cgagaagccc     1560

gcccuggaga uccggcagau ggugcucgcc gucuucgagg ugcuggggau cagcggcgcg     1620

auccuggacu ucgacgccga ggccgacggg aaggccuucg agcagcagcc cgccuaccag     1680

cucuggcacc uccuguacuc cgcggaggac gacggcaaga aguacagcca ggaggacauc     1740

uucaccuacg gccacgacaa caucgggcug aagaagaagc ugugcgagaa guucggcuuc     1800

cgcccggagc acaccaagau ccuggugaac gucguguucu ccgacgacua cgggaaccuc     1860

agcuccaagg ccaugcggcg caucuacccc uucaucaagg agcugacgua cgacaaggcc     1920

ugcgccgagg ccggcuaccg gcacagcgcg gccucccuga ccaaggagga gaucgccgcc     1980

cgcccccucc agaaccggcu ggaccugcuc aagaagaacg agcugcgcag ccccgucgug     2040

gagaagaucc ugaaccagau ggugaacguc gugaacaccc ucguggacga cgagaaccag     2100

aaccggaagg cccgcgggcu guccgaggac uuccacuucg acgagauccg gaucgagcug     2160

gcgcgcgagc ucaagaagaa cgccaaggag cgggccgaga ugaccacgaa caucaacacc     2220

gccaagcaga gccacgagaa gaucauccgc auccugcaga ccgaguucgg cgucccgaac     2280

cccucccgca acgacaucgu gcgguacaag cuguacgagg agcucaagaa caacggcuac     2340

aaggaccugu acaccaacac guacaucagc cgcgacaucc uguucuccaa gcagaucgac     2400

guggaccaca ucauccccca gaugagccuc uucgacgaca gcuucuccaa caagaccguc     2460

guguuccgga aggacaacca ggacaagggg aacaagaccg cguacgacua cauccugggc     2520

aaguacgggg agcaggcccu ggaggaguac aucagccgca ucaccucccu cuucgagcug     2580

ggcaagaaga acaaggagga gggcaucggg aaggccaagu uccagaagcu gcagaagcgg     2640

cucguggaca ucggcgacgg guucaucgag cgcgaccugc gggagagcca guacaucgcc     2700

aagaaggcca acaacaugcu guacgcgauc ugccgcuccg ucgugagcac cuccggcgag     2760

aucacggccc ggcuccgcga ggacugggac cuggugaacg ucaugcagga gaucaaccug     2820

cccaaguucc gggcccucgg gcugaccgag augguggaga agaaggacgg cgcccagaag     2880

gagcgcauca ccgacuggac caagcggaac gaccaccgcc accacgccau ggacgcgcug     2940

gccaucgccu ucacgcggca cagccacauc caguaccuca accaccugaa cgcccgcaag     3000

gacgaggcca acaaguggca cccggugguc uccaagaucg aggagaagga gaccgugcug     3060

aucuacgacg accucggcaa ccggaagcgc aaguucaagg cgcccaugcc caacuuccgg     3120

gaggugguca aggagcaccu ggagaacgug cugaucagcc acaaggccaa gaacaaggug     3180

gucaccaaga acaagaacaa gaucaagacc gugcgcgggg agaaggugaa gacggagcuc     3240

acgccccggg gccagcugca caaggagacc aucuacgggc gcuaccgcua cuacgagucc     3300

aaggaggaga agaucggccc gaaguucgac gccgagauga ucgggauggu caccaacccc     3360

caguacaaga agcugcuccu ggagcggcug gccgcgaacg gcaacgaccc caagaaggcc     3420

uucuggggca agaacgcccu caacaagaac ccccuguacg uggacgaggc ccgccaggag     3480

cugcucccgg agaagauccu gcugcggugg cucaacgacg acuacagcau ccgcaaggac     3540

gugacccccg agaaccugaa ggacaucaag accaucgaga agauccugga caaggggguc     3600

aagaagaucc uccugaagcg gcuggccgac uucggcaacg accccaagaa ggcguucucc     3660

gaccucgaca agaacccgau cuggcagaac gaggccaagc gcaucgccau caagcgggug     3720

accaucagcg ggaucaagaa cgccgcgccc cugcaccaca agaaggacca ccugggcaac     3780

gacauccucg acgagaaggg gaagcagauc cccuccagcu ucguguccac cggcaacaac     3840

caccacgucg ccaucuaccg cgacgccgag ggcaaccugc aggagcgggu ggugagccug     3900

uucgacgccg uccagcucgu gaacgggggc gagcccguga ucgacaagga guacaacaag     3960

gggaucggcu ggcaguuccu guucacgaug aagcagaacg agauguucgu cuucccggac     4020

gagaagaccg gguucaaccc ccgcgaggug gaccugcuca gcuccggcaa ccggaagcug     4080

aucagcccca accuguuccg cgugcagaag cucgccucca agaacuacuu cuuccggcac     4140

caccuggaga ccagcgucga ggagaagaag gagcugaaga acaucgccua caagccccag     4200

cucggccuga acgcgcuguc caacaucgug aaggugcgca ucaaccacau cggggagauc     4260

gucagcgugg gcgaguacug a                                               4281


<210>  2174
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2174
augaagaaca uccugggccu cgaccugggg aucaccagca ucuccuucgc ccacaucgug       60

gaggacgaga acaaggagaa guccgagauc aaggagcugg gcguccgcau cgugucccuc      120

accacggacg agcagagcga cuucgagaag gggaagucca ucaccaccaa cgccaaccgg      180

acccugaagc acggcgcgcg ccugaaccuc gaccgguacc agcagcgccg gaaguaccug      240

aucgaccugc uccagaaggc caaccugauc acgagcucca gcauccuggc cgagaacggg      300

aagaacacca cccacuccac cuggcagcuc cgcgccaagg ccgugacgga gcggaucgag      360

aaggaggagu ucgcgcgcgu ccugcuggcc aucaacaaga agcggggcua caagagcucc      420

cgcaaggcca agaccgagga cgagggccag gccaucgacg ggauggcgau cgccaagcgg      480

cucuacgacg agaaccugac ccccggccag cugagccucc agcugcugca gcagaacaag      540

aagcuccugc cggacuucua ccgcuccgac cugcagcggg aguucgaccu cguguggaac      600

uuccagaagc aguucuaccc cgacauccug accgacagcu ucuacaagga gcugcagggg      660

aagggcaagg acgccacguc caaggccuuc agcaagcgcu accacuucga caccgccgag      720

aacaaggggu ccaaggagag cgugcggcuc caggcguacc aguggcgcgc cgaggccauc      780

uccaagcagc ugagcaagga ggaggucgcc uacgugcuga ccgagaucaa caacaaccuc      840

aacaacgcca gcggcuaccu gggcgcgauc uccgaccgga gcaaggagcu guacuucaac      900

cgccagaccg uggggcagua ccucuacgcc cagcugcagg agaaccggca caacucccug      960

aagaacaagg ucuucuaccg ccaggacuac cucgacgagu ucgagcggau cugggagacg     1020

caggccagcu uccacaagga gcugaccgac gagcugaaga agcagauccg cgacguggug     1080

aucuucuacc agcgcaagcu caagucccag aagggccuga ucagcuucug cgaguucgag     1140

uccaaggaga ucgagaucga gaaggacggg aagaccauca ccaagacgau cggcgcccgg     1200

gucgugccca agagcagccc ccuguuccag gaguucaaga ucuggcagau ccucaacaac     1260

gugaccugca agcgcaaggg cauccggaag aagaaguccu ccgccaagag caccgagcug     1320

gaccuguuca acgagccguc ccagaccauc uucagccucg acauggagug caagcagcug     1380

cuguucgacg agcucaaccu gaagggggac cugaaguccg acaagguccu gaagcuccug     1440

ggcuacagcc cccaggagug ggagaucaac uacaaccagc uggaggggaa ccgcacgcag     1500

aaggcgcucu acgaggccua ccugaagauc guggagaugg aggcccacga cgugaaggac     1560

auccugcaga ucaaguccgc caaggacgac uggagccucg acgagagccc ccugagcgcc     1620

agcgagauca aggagaaggu caaggacauc uuccaggcgc ugggcaucug caccaagauc     1680

cucuacuucg acccccugcu gccggugaag gaguucgaga agcaggacuc cuaccagcuc     1740

uggcaccucc uguacagcua cgaguccgac gacagcaccu ccgggaacga gacccuguac     1800

cggauccugg agaagaagua cgccuucaag cgcgagcacg cccggauccu ggccaacgug     1860

gcgcuccagg acgacuacgg cagccugucc acgaaggcca uccgcaagau cuaccccaac     1920

aucaaggaga accaguacag caaggccugc gagcuggccg gcuacaagca cuccaagcuc     1980

agccugacca ccgaggagcu ggaggcccgg gagcucaaga acaucauccc ccugcugaag     2040

aagaacgcgc uccgcaaccc cgucguggag aagauccuga accagaugau caacgugguc     2100

aacgcccuga ucgagaagaa cuccgagcgg gacgccgagg ggaagaucac caaguacuuc     2160

cacuucgacg agauccgcau cgagcucgcc cgggagcuga agaagaacgc ccagaagcgc     2220

uacgagauga cgcagaacau caacaaggcg aagcuggagc accagaagau cagcgagauc     2280

cuccagaaga aguucggcau caagaacccg accaaguccg acaucauccg guaccgccug     2340

uaccaggagc uggagcacaa cggguacaag gagcucuaca ccaacgcccc caucgcccgg     2400

gacaugcugu ucagcaagaa caucgagauc gagcacaucg ugcccaaggc ccgcgucuuc     2460

gacgacuccu ucagcaacaa gacccugacg uuccaccgga ucaacuccaa caagggcgag     2520

uacaccgccu ucgacuacau caccagccuc aacagcgagg aggagcugaa ccaguaccug     2580

acccgcgugg agaacgcgua caagaccaag uccaucagcc ucacgaagua caagaaccug     2640

cugaagaagg ccuccgagau cggggacggc uucaucaacc gcgaccuccg ggacacccag     2700

uacaucgcca agaaggccaa ggagauccug uuccagguga ccaagaacgu ccugagcacc     2760

uccggcagca ucacggaccg ccuccgggag gacugggggc ugguggacgu gaugaaggag     2820

cugaacaugc ccaaguacca gucccucggc cugaccgagg ucgaggagcg caaggagggg     2880

aacaagguga ccgugaucaa gaacuggacc aagcggaacg accaccgcca ccacgcgaug     2940

gacgcccuga cggucgccuu caccaagccg agcuacaucc aguaccucaa ccaccugaac     3000

gcccggaagg acgagaacaa caagaacuac uccgugaucc uggccaucga ggagaaggag     3060

accaucaagg ugcccaccaa caacggcaag aacaagcgcg ucuucaucga gcccaucccc     3120

aacuuccgcc agguggcgaa gaagcaccuc gaggagaucu ucaucagcca caaggccaag     3180

aacaaggugg ucacgaagaa cgugaacaag ccggccggga ccgacaagca gcagaucacc     3240

cugacgcccc ggggccagcu gcacaaggag accaucuacg gcaaguacca guacuacgug     3300

aacaaggagg agaaggucgg ggugaaguuc gacgagcgga ccaucaccaa gguguccaac     3360

ccccucuacc gcgaggcccu gcugaagcgg cuccaggcca acgacaacga ccccaagaag     3420

gcguucaccg gcaagaacgc ccugagcaag aacccgaucu accuggacga gacgaagacc     3480

aagacccucc ccgagaaggu caaccugacc uaccuggagg aggacuucuc cauccgcaag     3540

gacaucagcc ccgacaacuu caaggaccuc aaguccaucg agaaggugau cgaccagggg     3600

gugaagcgga uccugaucaa gcgccugcag gacuacgaca acgaccccaa gaaggccuuc     3660

gucgaccucg agaagaaccc gaucuggcug aacaaggaga agggcaucgc caucaagcgg     3720

gugacgauca gcggcguguc caacgcccag ccccugcaca ucgggaagga ccaccucggc     3780

aagaccaccc ugaacaagga ggggaacgag auccccgucg acuacgugag caccggcaac     3840

aaccaccacg uggcgaucua ccgcgacaag gaggggaacc ugcaggagca gaucgucucc     3900

uucuucgagg ccguggugcg gacgcagcag ggccucccga ucaucgacaa gaccuacaag     3960

caggaggagg gcuggcaguu ccuguucacc augaagcaga acgagauguu cgucuucccc     4020

aacgccacca cgggguucaa ccccgccgag aucgaccugc ucgaccccaa gaacaagaag     4080

cugaucagcc cgaaccuguu ccgcgugcag aagaucgcga ccaaggacua cuucuuccgg     4140

caccaccucg agaccaacgu ggagaccgac aacguccuga agaacacgac cuggaagcgc     4200

gagggccuga gcgggcucaa ggacaucgug aaggugcgca ucaaccaccu gggcgacauc     4260

gucuccaucg gggaguacug a                                               4281


<210>  2175
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2175
augaagcaca uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucaag       60

caggacuucg agaacaagca gggggagauc cucggcaugg ggucccgcau cauccccaug      120

agccaggaca uccugggcga cuucggcaag gggaacuccg ugagccagac cgccgagcgg      180

acgaaguacc gcuccguccg gcgccugcgg gagcgcuucc uccugcggcg cgagcggcug      240

caccgcaucc ucaacgugcu gggcuuccug ccggagcacu acgcgagcca gaucgacuuc      300

accaagcggu ucgggaaguu caaggaggag accgagccca agcucgugua cuccaacgag      360

ggcuucaucu ucaagaaguc cuucgaggag augcugagcg acuucaagac ccaccagccc      420

cagcugcucg aggacaacaa gaagaucccc uacgacugga cgaucuacua ccugcgcaag      480

aaggcccuga gccggaagau cgagaaggag gagcucgccu ggauccugcu gaacuucaac      540

cagaagcgcg gguacuacca gcuccggggc gaggaggagg aggagaaccc gaacaagcug      600

aucgaguucu acucccugaa gaucgucgac gugcucgccg acgagcccca gaagggcaag      660

agcgacaucu gguacucccu gauccuggag aacgggugga ucuaccgccg gagcuccaag      720

acgccccucu ucgacuggaa ggagaagacc cgcgacuuca ucgugaccau cgaccugaac      780

gacgacggca gcgucaagac cgacaaggag gggaacgaga agcgguccuu ccgcgccccc      840

ggcgagaacg acuggacccu ggugaagaag aagaccgagc aggagaucga cagcucccgg      900

aagaccgugg gcacguacau cuacgagacc cuccugaaga acccgaagca gaagaucaag      960

gggaagcugg uccgcaccau cgagcgcaag uucuacaaga ccgagcucaa gcagauccug     1020

gagaagcaga aggaguucca ccccgagcug cggcaggacg accucuacag cgacugcgug     1080

cgcgagcugu acaagaacaa cgaggcgcac cagcugacgc ucagcaagaa ggacuucgug     1140

caccuguuca uggaggacau caucuucuac cagcggcccc ugggcuccca gaaguccagc     1200

aucagcaacu gcacccucga guuccgcaag uacaaggacg agaacgggac cgagcacacc     1260

caguaccuga aggccauccc caaguccaac ccguacuacc aggaguuccg gaucuggcag     1320

uggauguaca accugaacau cuacaagaag gaggacgaca cgaacgucac caaggacuuc     1380

cucaacacca aggccgacuu cgagagccug uucgaguucc ugaacaaccg caaggaggug     1440

gaccagaagg cccuccugaa gcacuucaag cugaacgaca agacccaccg guggaacuac     1500

guggaggaca agaaguaccc cugcaacgag acgaagacca ugauccuguc ccgccucgac     1560

aaggucgaga acaucagcga cgacuuccug acccgggaga uggagcagaa gaucuggcac     1620

aucaucuacu ccgugaacga caagguggag uacgagaagg cgcucaagag cuucgccggc     1680

aagaacaacc uggaggacaa cuccuucuuc gaggccuuca agaaguuccc gcccuucaag     1740

agcgaguacg gggccuucuc cgagaaggcc aucaagaagc ugcugccccu caugcgcaug     1800

ggcaaguucu ggaacuacaa caacaucagc gagcagucca agagccggau ccagaagauc     1860

aucaccggcg aguacgacga gaacaucaag gacaaggucc gcgagaaggc gauccaccug     1920

acgaccgaga ccgacuucca ggggcugcag cucuggcugg ugcaguacau cguguacggc     1980

cggcacuccg aggccgccau gcuggggaag uggaacagcg ccgacgaccu cgaggaguuc     2040

cugaaggagu ucaagcagca cucccugcgc aaccccaucg ucgagcaggu caucaccgag     2100

acgcuccggg uggugaagga caucuggcag aaguacggca acggggccaa ggacuucuuc     2160

cgcgagaucc acaucgagcu gggccgggag augaagcugc ccgcggagga gcgcgagcgg     2220

cucacccgcc agaucagcga gaacgagaac accaacuucc gcaucaaggc ccugcuggcc     2280

gagaugaugg acgacaacag cguggagaac guccggcccu ucuccccgau gcagcaggag     2340

auccucaaga ucuacgagga gggcgugcug aacagcgaca ucgagaucga ggacgacauc     2400

cugaagaucu ccaagaccgc ccagcccagc uccagcgacc ucaagcgcua caagcugugg     2460

cuggagcaga aguacaaguc ccccuacacg gggcagauca ucccccucaa caagcuguuc     2520

accccggagu acgagaucga gcacaucauc ccccagagcc gguacuucga cgacuccuuc     2580

accaacaaga ucaucugcga gagcgccgug aacaagcuga aggacaacua caucggccuc     2640

gaguucauca agaagcucgg gggcuccauc gucgagugcg ggaacggcaa gagcguggcg     2700

auccugaagc acgacgccua cgaggacuuc aucaagaagc acuacgccaa caaccgcucc     2760

aagcggaaca agcugcugcu ggaggacauc cccgagaaga ugaucgagcg ccagaugaac     2820

gacacccggu acaucagcaa guacaucucc ggcgugcuca gcaacaucgu ccgcguggag     2880

gacgggaccg acgagggcgu gaacuccaag aacaucgucc ccgggaacgg caagaucacg     2940

acccagcuga agcaggacug gggccugaac gacgugugga acgaccucau ccugccgcgg     3000

uucgagcgca ugaaccaccu gaccaacagc accgacuuca cggccuggaa cgagaaccac     3060

cagaaguucc uccccaccgu gcccgucgag uucagcaagg gguucuccaa gaagcggauc     3120

gaccaccgcc accacgcccu ggacgcgcug gugaucgccu gcgccaccaa ggaccacgug     3180

aaccuccuga acaaccagag cgccaagucc gacaccaagc gguacgaccu gaagaagaag     3240

cucaugaagu ucgagaaggu cgcguacaac cacccgcaga cgggcgagcg caucgagcgg     3300

gaggccccca agcaguuccu gaagcccugg gagaccuuca ccaucgacgc caagaaccac     3360

cuggagacca ucaucgugag cuucaagcag aaccuccgcg ugaucaacaa ggccacgaac     3420

uacuacgaga aguacgucga gaaggagggg accaagguga aggagcgggu ggagcagaag     3480

ggcaccaacu gggccauccg caagcccaug cacaaggaga ccgucuccgg gaagaucgac     3540

cugccgcgga ucaaggugcc caagggcaag auccugacgg cgacccgcaa gagccucgac     3600

accuccuuca accugaagac caucggcagc aucacggaca ccgggaucca gaagauccug     3660

aagaacuacc uccaguacaa ggacaacaac cccgagcugg ccuucucccc cgagggcauc     3720

gaggacauga acaagaacau cgagaaguuc aacaacggga aggcccacca gagcaucucc     3780

aaggugcggg ucuucgagcu gggcagcaag uucccgcucg ggcaguccgg caacaagaag     3840

gagaaguacg uggaggccgc caagggcacc aaccuguucu ucgcgaucua ccaggacaag     3900

aacgggaagc gcagcuacga gaccaucccc cugaacgagg ugaucgagcg ccagaagcag     3960

ggccucuccg ucguggaccu gaagggggug gacgacuucu accugagccc caacgaccuc     4020

gucuacgugc ccuccgagga cgagcuggag aacaucagca ugaucgacuu caacaagaug     4080

uccagcgagc ggaucuacaa gguggucagc uucacgggca accagaucuu cuucgugcgc     4140

caggacaucg ccaccuccgu ggucaacaag gccgaguuca gcacccugaa caagauggag     4200

cgggccaucg acgggaccau gaucaaggag acgugcguga agcuccagau cgaccgccug     4260

ggcaacaucu ccaagaucug a                                               4281


<210>  2176
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2176
auguacaaca ucggccugga caucgggacc ggcagcgugg gguggugccu caccgacgag       60

aacggcuacc ugcugaaggu caaccgcaag gggaacaacg gcaacacgua ccggaacucc      120

gccuggggcg ugcgccucuu cgagagcgcc gacaccgcgg ccgacugccg gaucaagcgc      180

uccacccggc gccgguacaa gcgccggcgc acccggauca ucgagcugcg caagaucaug      240

agcgacauga ucaugcccau cgacccgaac uucuacgccc ggcuggacga ggccuuccuc      300

uggaacgagg acaaguccga caaggccaag gcgcccuucc ugcuguucaa cgacaacggg      360

uacgacgacg ugaaguacua cacggacuac cccaccaucu accaccuccg caagcaccuc      420

cuggagacca aggagaaggc cgacccccgg uucaucuacc uggcccugca ccacaugaug      480

aaguaccgcg gccacuuccu guucgagggg cagagcuucg aggccaucga caacaucgag      540

gacaccuuca ucgagcucga gcaccugguc aacguguacg ugaaggagaa ggaggacacg      600

gacaacaacg cggagaacaa cgcccuguac caggagauga agaacuaccu cgccgacaac      660

aaggucaaga acaaggacaa gaaggaguac aucaccgaca ccuucaucaa ggccgacuac      720

gacaacaagu acuccaagga gcuggccgcg gccgugcugg gcuacgaguu caacgugggg      780

aucaucguca acgacaacag ccucaccgac gaggacggca aggcccugaa ggccaaguuc      840

gccgacgcga aguacgagga gaaggaggag aagcuguccg acacgcucgg cgagcgguac      900

uacaucaucg agacccugaa gaagaucuac agcuggaagg ugcugcacuc cauccucggg      960

gacaacaagu accugagcua cgccauggug gacaaguacg agaagcacuc cgagcagcug     1020

aaggcccuca aguaccuguu ccacaaguac accagccagg acgaguacuc cgaguucuuc     1080

caccaggaga agaacaagga gggcaaguac aucgucaacu acgccaacua caucaagggg     1140

aucaagcgcc ugagcaacga gaccaacaag aaguacaaca cgaagcagca gcucuaccag     1200

uccaucauga agauccuggg cgagcgggcc gcggacgacg agguguacaa gaagauccug     1260

guggagaugg agcaggagac cuuccucgag aagaucaaca acgucgacaa cagcgccauc     1320

ccguaccagc ugaaccugau ggagauggac aagauccuca cccagcaggg cguguacuac     1380

aaggagcugc gcgacaacaa ggagcugcuc cugaagaugc ugaccuccaa gauccccuac     1440

uacguggggc cccucaacaa caacagcaac ggcaaccgga acuucgccug gaugacgaag     1500

aaggacggga aggagaacga gaaggucuac cccuggaacg ugaaggacgu ggucgacauc     1560

gacgugaccg ccgaggacuu caucacccgc augaccaacu acugcacgua ccugccgaac     1620

gagaaggugc ugcccaagga gagccuccug uaccagcgcu acaugcugcu ggaggagcuc     1680

ucccagaucc ggaucgacgg ccgcaagcug agcaaggagg accggaaggc caucaucaac     1740

gaccuguuca ucgggaaggg ccgcgucaag guguccgaca aggacuucaa ggaguaccuc     1800

aacaagguca acuacgugaa ggugaacggc aagggguacg acgucaccgg cuaccagagc     1860

gacgacgggu ucgcgugcgc ccuguccagc uacaacaagu uccggcgcau ccugggccac     1920

aucgacgagc agaacgagaa gaugaucgag gacaucaucu acuggaucac cguguucgag     1980

gacaaggaca ucgugaagcg gaagaucaac aagcaguacg ccgaccgccu cuccgccgag     2040

cagcugaaga agauccugaa gcucaaguuc aagggguggg gccgguacag cuccaagcug     2100

cugaacggca ucaaggggga caugggcacc acgaucaucg agaugcucga ggacgcggac     2160

gagcgcuucg ccuacugcga cuacugcccc aacuucaugc agaucaucaa caaggacgag     2220

aagaucaagc agaucaucga cgacaaccgg ccccgcuacg acgggaccaa ggaccugcug     2280

gacgucaucc aggacaugca caccagcccg gccaaccggc gcggcaucug gcagaccaug     2340

aaggccaucg aggagaucau cgaguacaug ggggagaagc cccagcagau cuacaucgag     2400

uucgcccggg aggacgaccc caaggcgaag aacaagcgcg ccgacucccg gaagcgcgcc     2460

aucgacaagg cccucaagaa gcugaaggag gacguggugg acgaguacaa cgagaagguc     2520

uacaaggagc ugaagcagua cgagaagcgg cucgacgagg agaaggugua ccuguacuuc     2580

augcagaacg gcaaggaccu guacuucccc gacaaggagc ucaaccugga cgagcuggag     2640

aagcucgaga ucgaccacau caucccguac agccuguccg acgacgacag ccuggacaac     2700

aaggcccucg ugcugaaggg caacaaccag aacaagggga acaagaucgu caaggaggcg     2760

uucccccagc uguucuccaa cagcggcacg gugaacuacu ggaagaaccu caagaaggcc     2820

gggcugaucu ccgagaagaa guacaacaac cugcagaaga acaacgugga cgacauccuc     2880

accaagggcu ucaucaaccg ccagcugguc gagacccgcc agaucgucaa gagcguggcc     2940

aaccugaucc ucgacuacua caacgagcag aucgacguga ucgaggugaa ggccucccug     3000

agcaccagcg ugcggaagau gcugacguac gagaagaagg acaacaacgg cuucuggauc     3060

gagaacaagg acaacugcau guucuacaag aaccgcgaca ugaacgacua ccaccacgcc     3120

cacgacgcgu accucgccaa caucaucggg cuguacaucc agaagaacua cccccggcug     3180

cagaaggagc ucaacuacuc ccaguaccgc cggaucuggc gcaaguacua cgagaacacc     3240

aagaacaaca acggcgucaa cugguucgcc acccugggga aguucagcuc caacaacgag     3300

gaggccgacu gguacggcca ggacaucauc gcguacaugc ggaagaucuu cugcuaccgc     3360

gacgugcuga ucagcaagaa gguggaggag aacaccgggg ccuucuacuc cgagaccaag     3420

uacccccggg aggacaaggc cggcagcaag cucgucccgc ugaagcaggg caacaacaug     3480

cgcggggcca acaaccugaa ggagcucgac gcccggaagu acggcgggua caagggcggg     3540

gagaaggcgu acuucgugcu ggugaaguac ugcaucgaga aggcccugaa gaagaagguc     3600

aagaaggagu accacaugga guucguggag auccccgugu acaucgcccg cggcaucaag     3660

aacaaggaca ucaaccucua cgacuacguc ugcgacaucc ugaacgaggc caacaagaac     3720

uccguggccg acguggagau ccugcggaac aaggucccca aguaccagau gaucaucggc     3780

gagaacgggg aggaguacua ccucgugagc gcgacggagg ugaucaacuc caagcaguuc     3840

guccugggcg gggccaacca gcaguacaac cgccugcuca acuacaucaa guacggcaag     3900

aacgacaagu ggcaguacau ccagaccgag cugcuggacg agcagcucau cgggcuguac     3960

aaccugcuca ucgccaagau caaggacgag uacaagggcu ucagcaagga ggccgugcgg     4020

auccaggaga acaacuccuu cgacaagcug gacgugaagg acaagaagga guucaucgcc     4080

gagaugauca agcuggucca gcccgacagc gacuacccgu accucggcaa guacgcgacc     4140

gggcugucca accgcauggg ccggaagacc ggggagaagg ugggcaagaa gaucacgcug     4200

guggacaaga gcgucaccgg gcucuacgag cgccggacca ccuucgagcu ggaggacgac     4260

uccagcacga aguccugcug a                                               4281


<210>  2177
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2177
augaagaaca uccugggccu cgaccugggg accaccagca ucggcuucgc ccacaucgug       60

gaggacgaga acaaggagaa guccgagauc aaggagcugg ggguccgcau cgugagccuc      120

acgaccgacg agcaguccga cuucgagaag ggcaagagca ucaccaccaa cgccaaccgg      180

acgcugaagc acggggcgcg ccugaaccuc gaccgguacc agcagcgccg gaaguaccug      240

aucgaccugc uccagaaggc caaccugauc acccccucca gcauccuggc cgagaacggc      300

aagaacacca cccacuccac guggcagcuc cgcgccaagg ccgugaccga gcggaucgag      360

aaggaggagu ucgcgcgcau ccugcuggcc aucaacaaga agcggggcua caagagcucc      420

cgcaaggcca agaccgagga cgaggggcag gccaucgacg gcauggcgau cgccaagcgg      480

cucuacgacg agaaccugac cccggggcag cugagccucc agcugcugca gcagaacaag      540

aagcuccugc ccgacuucua ccgcuccgac cugcagaagg aguucgaccu cgucuggaac      600

uuccagaagc aguucuaccc cgacauccug acggacaucu ucuacaagga gcugcagggc      660

aaggggaagg acgccaccag caaggccuuc uccaagcggu accacuucga caccaccgag      720

aacaagggca gcaaggaguc cgugcgccuc caggccuacc aguggcgggc ggaggccauc      780

agcaagcagc uguccaagga ggagguggcc uacguccuga cggagaucaa caacaaccuc      840

aacaacgcca gcggcuaccu gggggccauc uccgaccgca gcaaggagcu guacuucaac      900

cggcagaccg ugggccagua ccucuacgcg aagcugcagg agaaccgcca caacagccug      960

aagaacaagg uguucuaccg gcaggacuac cucgacgagu ucgagcgcau cugggagacc     1020

caggccuccu uccacaagga gcugaccgac gagcugaaga agcagauccg ggacgucgug     1080

aucuucuacc agcgcaagcc caagagccag aaggggcuca ucuccuucug cgaguucgag     1140

agcaaggaga ucgagaucga gaaggacggc aagacgauca ccaagaacau cggcgcccgc     1200

guggucccga aguccagccc ccuguuccag gaguucaaga ucuggcagau ccugaacaac     1260

gugaucugca agcggaaggg gauccgcaag aagaagaucu ccgccaagac cacccagcuc     1320

gaccugcuga acgagagcuc ccagacgauc uucagccucg acauggagug caagcagcug     1380

cuguucgacg agcucaaccu gaagggcgac cugaaguccg acaaggugcu gaagcuccug     1440

ggguacagcc cccaggagug ggagaucaac uacaaccagc uggagggcaa ccggacccag     1500

aaggcccucu acgaggcgua ccugaagauc gucgagaugg aggcccacga cgugaaggac     1560

auccugcaga ucaaguccgc caaggacgac uggagccucg acgagagccc ccuguccgcc     1620

uccgagaucc gcgagaaggu gaaggccauc uuccagaccc uggggaucug caccaagauc     1680

cucuacuucg acccgcugcu gcccgucaag gaguucgagg agcaggacag cuaccagcuc     1740

uggcaccucc uguacuccua cgagagcgac gacagcacgu ccggcaacga gacccucuac     1800

cggauccugg agaagaagua cgcguucaag cgcgagcacg cccggauccu ggccaacgug     1860

gcccuccagg acgacuacgg cagccugucc accaaggcga uccgcaagau cuaccccaac     1920

aucaaggaga accaguacag caccgccugc gagaaggccg gguacaagca cuccaagcug     1980

agccucacga ccgaggagcu ggaggcccgg gagcugaaga acaucauccc ccuccugaag     2040

aagaacgccc ugcgcaaccc gguggucgag aagauccuca accagaugau caacguggug     2100

aacgcgcuga ucgagaagaa cuccgagcgg gacgccgagg gcaagaucac caaguacuuc     2160

cacuucgacg agauccgcau cgagcuggcc cgggagcuca agaagaacgc ccagaagcgc     2220

uacgagauga cccagaacau caacaaggcc aagcuggagc accagaagau cagcgagauc     2280

cugcagaagg aguucgggau caagaacccc acgaaguccg acaucauccg guaccgccuc     2340

uaccaggagc uggagcacaa cggcuacaag gagcuguaca ccaacgcgcc caucgcccgg     2400

gacaugcugu ucagcaagaa caucgagauc gagcacaucg uccccaaggc ccgcguguuc     2460

gacgacuccu ucagcaacaa gacccugacc uuccaccgga ucaacuccga caagggggag     2520

uacaccgccu ucgacuacau cacgagccug aacuccgagg aggagcucaa ccaguaccug     2580

acccgcgucg agaacgccua caagaccaag agcaucuccc cgaccaagua caagaaccug     2640

cucaagaagg cgagcgagau cggcgacgac uucaucaacc gcgaccugcg ggacacgcag     2700

uacaucgcca agaaggccaa ggagauccug uuccagguga ccaagaacgu gcucagcacc     2760

uccggcagca ucaccgaccg ccugcgggag gacugggggc uggucgacgu gaugaaggag     2820

cucaacaugc ccaaguacca gucccugggc cugacggagg uggaggagcg caaggacggg     2880

aacaagguca ccgugaucaa gaacuggacc aagcggaacg accaccgcca ccacgccaug     2940

gacgcgcuca ccguggccuu cacgaagccc agcuacaucc aguaccugaa ccaccugaac     3000

gcccggaagg acgagaacaa caagaacuac uccgucaucc ucgccaucga ggagaaggag     3060

accaucaagg ugcccaccaa caacggcaag aacaagcgcg uguucaucga gccgaucccc     3120

aacuuccggc aggucgccaa gaagcaccug gaggagaucu ucaucagcca caaggcgaag     3180

aacaaggugg ugaccaagaa cacgaacaag cccgccggga ccgacaagca gcagaucacc     3240

cugacgcccc gcggccagcu ccacaaggag accaucuacg gcaaguacca guacuacauc     3300

aacaaggagg agaagaucgg ggucaaguuc gacgagcgga ccaucgccaa gguguccaac     3360

ccgguguacc gcgaggcccu gcugaagcgg cuccaggcca acgacaacga ccccaagaag     3420

gcguucgccg gcaagaacgc ccugagcaag aaccccaucu accuggacga guccaagacc     3480

aagacccucc ccgagaaggu caaccugacg uaccuggagg aggacuucag cauccgcaag     3540

gacaucuccc cggacaacuu caaggaccuc aagagcaucg agaaggugau cgaccagggg     3600

gugaagcgga uccugaucaa gcgccugcag gccuacgaca acgaccccaa gaaggccuuc     3660

gucgaccucg agaagaaccc caucuggcug aacaaggaga agggcaucgc gaucaagcgg     3720

gugaccaucu ccggcgugaa caacgcccag ccgcugcaca ucgggaagga ccaccucggc     3780

aagaccaccc ugaacaagga ggggaaggag auccccgucg acuacgugag cacgggcaac     3840

aaccaccacg uggccaucua ccgcgacaag gaggggaacc ugcaggagca gaucgucucc     3900

uucuucgacg ccguggugcg ggcgcagcag ggcaucccca ucaucgacaa gaccuacaag     3960

caggccgagg gcuggcaguu ccucuucacc augaagcaga acgagauguu cgucuucccc     4020

aacgccacca cgggguucaa cccggccgag aucgaccugc uggaccccaa gaacaagaag     4080

cucaucagcc ccaaccuguu ccgcgugcag aagaucgcca ccaaggacua cuucuuccgg     4140

caccaccugg agaccaacgu ggagaccgac aacauccuca agaacgucac guggaagcgc     4200

gagggccugu ccgggcugaa ggacaucgug aaggugcgca ucaaccaccu cggcgacauc     4260

gucagcaucg gggaguacug a                                               4281


<210>  2178
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2178
augaagaaca uccugggccu cgaccugggg accaccagca ucggcuucac gcacaucgug       60

gaggacgaga acaaggagaa guccgagauc aaggagcugg ggguccgcau cgugagccuc      120

accaccgacg agcaguccga cuucgagaag ggcaagagca ucuccaccaa cgccaaccgg      180

acgcugaagc acggggcccg ccugaaccuc gaccgguacc agcagcgccg gaaguuccug      240

aucgaccugc uccagaaggc gaaccugauc accagcucca gcauccuggc cgagaacggc      300

aagaacacca cccacuccac guggcagcuc cgcgccaagg ccgugaccga gcggaucgag      360

aaggaggagu ucgcccgcgu ccugcuggcg aucaacaaga agcggggcua caagagcucc      420

cgcaaggcca agaccgagga cgaggggcag gccaucgacg gcauggccau cgcgaagcgg      480

cucuacgacg agaaccugac ccccgggcag cugagccucc agcugcugca gcagaacaag      540

aagcuccugc cggacuucua ccgcuccgac cugcagaagg aguucgaccu cguguggaac      600

uuccagaagc aguucuaccc cgacauccug acggacagcu ucuacaagga gcugcagggc      660

aaggggaagg acgccaccuc caaggccuuc agcaagcggu accacuucga caccgccgag      720

aacaagggcu cccgcgagag cgugcggcuc caggccuacc aguggcgcgc ggaggccauc      780

uccaagcagc ugagcaagga ggaggucgcc uacgugcuga ccgagaucaa caacaaccuc      840

aacaacgcca gcggcuaccu gggggccauc uccgaccgga gcaaggagcu guacuucaac      900

cgccagacgg ugggccagua ccucuacgcg cagcugcagg agaacaagca caacucccug      960

aagaacaagg ucuucuaccg gcaggacuac cucgacgagu ucgagcgcau cugggagacc     1020

caggccagcu uccacaagga gcugaccgag gagcugaaga agcagauccg ggacguggug     1080

aucuucuacc agcgcaagcu caagucccag aaggggcuga ucagcuucug cgaguucgag     1140

uccaaggaga ucgagaucga gaaggacggc aagaccauca cgaaguccau cggcgcccgc     1200

gucgugccca aguccagccc ccuguuccag gaguucaaga ucuggcagau ccucaacaac     1260

gugaccugca agcggaaggg gauccgcaag aagaagucca gcgccaaguc caccgagcug     1320

gaccuguuca acgagccgag ccagaccauc uucucccucg acauggagug caagcagcug     1380

cuguucgagg agcucaaccu gaagggcgac cugaaguccg acaagguccu gaagcuccug     1440

ggguacagcc cccaggagug ggagaucaac uacaaccagc uggagggcaa ccggacgcag     1500

aaggcccucu acgaggcgua ccugaagauc guggagaugg aggcccacga cgugaaggac     1560

auccugcaga ucaagagcac caaggacgac ugggcccucg aggagagccc ccugagcgcc     1620

agcgagaucc gcgagcgggu caaggccauc uuccagaccc uggggaucug caccaagauc     1680

cucuacuucg acccccugcu gccggugaag gaguucgagg agcaggacuc cuaccagcuc     1740

uggcaccucc uguacagcua cgaguccgac gacagcacgu ccggcaacga gacccuguac     1800

cgcauccugg agaagaagua cgcguucaag cgggagcacg ccaccauccu ggccaacgug     1860

acccuccagg acgacuacgg cagccugucc acgaaggcca uccgcaagau cuaccccuac     1920

aucaaggaga accaguacag caccgcgugc gagaaggccg gguacaagca cuccaagcug     1980

agccucacca ccgaggagcu ggaggcccgg gagcugaaga acaucauccc ccuccugaag     2040

aagaacgccc ugcgcaaccc cgucguggag aagauccuca accagaugau caacgugguc     2100

aacgcccuga ucgagaagaa cuccgagcgg gacgcggagg gcaagaucac gaaguacuuc     2160

cacuucgacg agauccgcau cgagcuggcc cgggagcuca agaagaacgc ccaggagcgc     2220

uacgagauga cccagaacau caacaaggcc aagcuggagc accagaagau cagcgggauc     2280

cugcagaagg aguucggcau caagaacccg accaaguccg acaucacccg guaccgccuc     2340

uaccaggagc uggcccacaa cggguacaac gaccuguaca ccaacacgua cauccccaag     2400

gagcuccugu ucagcaagaa caucgacauc gagcacaucg uguccaaggc gcgggucuuc     2460

gacgacagcu ucuccaacaa gacccuggcc uuccgcgagu ucaacaucaa gaagggcgag     2520

agcaccgccu ucgacuacau caccagccuc aacaacgagg aggagcugaa ccaguaccug     2580

aaccgggugg agaacgccua caagacgaag aacaucuccc ccgccaagua caagaaccuc     2640

cugaagaagg cgagcgagau cggcgacggg uucaucaacc gcgaccugcg cgacacccag     2700

uacaucgcca agaaggccaa ggagauccuc uuccagguga ccaagaacgu ccuguccacc     2760

agcggcucca ucacggaccg gcugcgcgag gacugggggc ucguggacgu gaugaaggag     2820

cugaacaugc ccaaguacca gagccugggc cucaccgagg ucgaggagcg gaaggagggg     2880

aacaagguga ccgugaucaa gaacuggacc aagcgcaacg accaccggca ccacgccaug     2940

gacgcgcuga cggucgccuu caccaagccg uccuacaucc aguaccugaa ccaccucaac     3000

gcccgcaagg acaagaacaa caagaacuac agcgugaucc uggccaucga ggagaaggag     3060

accaucaagg ugcccaccaa caacggcaag aacaagcggg ucuucaucga gcccaucccc     3120

aacuuccgcc agguggccaa gaagcaccug gaggagaucu ucaucuccca caaggcgaag     3180

aacaaggugg ucacgaagaa caccaacaag ccggccggca ccgacaagca gcagaucacc     3240

cucacgcccc gggggcagcu gcacaaggag accguguacg gcaaguacca guacuacgug     3300

aacaaggagg agaagaucgg ggucaaguuc gacaaggaga ccaucgccaa ggugagcaac     3360

cccguguacc gcgaggcccu gcucaagcgg cugcaggcca acgacaacga ccccaagaag     3420

gcguucgccg gcaagaacgc ccuguccaag aacccgaucu accucgacga ggccaagacc     3480

aagacgcuga ccgagaaggu caaccugacc uaccucgagg aggacuucag cauccgcaag     3540

gacaucuccc ccgacaacuu caaggaccuc aagagcaucg agaaggugau cgaccagggc     3600

gugaagcgga uccugaucaa gcgccugcag gacuacgaca acgaccccaa gaaggccuuc     3660

gucgaccugg agaagaaccc caucuggcug aacaaggaga aggggaucgc gaucaagcgg     3720

gugaccaucu ccggcgugaa caacgcccag ccgcuccaca ucgggaagga ccaccugggc     3780

aagacgaccc ugaacaagga ggggaaggag auccccgucg acuacgugag caccggcaac     3840

aaccaccacg uggccaucua ccgcgacaag gagggcaacc uccaggagca gaucgucucc     3900

uucuucgagg ccgugguggg ggcgcagcag ggccugccca ucgucgacaa gaccuacaag     3960

caggccgagg gguggcaguu ccuguucacg augaagcaga acgagauguu cguguucccg     4020

aacgaggcca ccggcuucaa ccccgccgag aucgaccucc uggaccccaa gaacaagaag     4080

cagaucagcc ccaaccuguu ccgggugcag aagaucgcca ccaaggacua cuucuuccgc     4140

caccaccucg agaccaacgu cgacacggac aacgugcuga agaacaccac cuggaagcgg     4200

gaggggcugu ccggccucaa ggacaucgug aagguccgca ucaaccaccu gggcgacauc     4260

gugagcaucg gggaguacug a                                               4281


<210>  2179
<211>  4284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2179
augaagaaga uccugggccu cgaccugggg accagcucca ucggcugggc ccuggugaac       60

gaggccgaga ccagcgagga gaaguccagc aucaucaagc ucgggguccg cgugaacccc      120

cugacggugg acgagcugca gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggaccgccg ggcgcagcau gcggcgcaac cuccagcggu acaagcugcg ccgggagaac      240

cugaucgagg uccucaagga guacaacuuc aucacggacg agaugauccu guccgagaac      300

ggcaaccgca ccaccuucga gaccuaccgg cugcgcgcca aggccgccgu ggaggagauc      360

agccucgagc aguucgcgcg ggugcugcug augaucaaca agaagcgcgg cuacaagucc      420

agccggaagg ccaagggggc cgagaacggc acgcucaucg acgggaugga ggucgccaag      480

aagauguacg acgagggcau caccccgggg gagcugugcc ugcagcuccu gaccgcgggc      540

aagcgcuacu uccccgacuu cuaccggucc gaccugcagg ccgaguucga ccgcaucugg      600

aacuuccaga agcaguucua ccccgacagc cucaucgaca aggugaagga cgaggugcgg      660

ggcaagaaca agucccagac cugggccauc cuggccaagu acuucgucug gaaggaggug      720

gagaacagcu ggaacgagga ggaggcccag acgcgccggg uggagaagga guaccgccug      780

gucgggauca agcggggcgu gaagcgcgag gagcucaagc uggagaacuu ccaguggcgc      840

gugaaggcgc uguccgagcg gaugaacccc gaggagcucg ccaucguccu gcaggagauc      900

aacgagcaga ucagcaacuc cagcggguac cugggcgcca ucuccgaccg cagcaaggag      960

cucuacuuca accggcagac cgugggccag uaccagaugg ccgugcugga caacaacccg     1020

aacaucgggc ugcgcaacau ggucuucuac cggcaggacu accucgacga guucaacacc     1080

aucugggaga agcaggccga guuccacaag gagcugaccg aggaccugaa gaaggagauc     1140

cgcgacaucg ugaucuucua ccagcggcgc cucaagagcc agaagggccu gaucaacauc     1200

ugcgaguucg agcggcgcca gaucgaggug gagaucgacg ggaagaagaa gaucaagacg     1260

aucggcuccc gggucauccc ccgcagcagc ccccuguucc aggaguucaa gaucuggcag     1320

acccucaacg acaucgaggu guccgugcug ggggucaaga acaagcggaa gaagcaggac     1380

gacaacucca ccacccugcu cgacagcgcg gagaacaucg acucccugaa gcugaacgug     1440

agccgccccc ucgacgccga cgagaagucc cugcuggcca aggagcuguu cauccgggac     1500

aagcugacca agagcgacgu ccugaagcuc cucuucaaca acccgcagaa ccugaaccug     1560

aacuucaaga acaucgacgg caaccgcacc ggcuccgccc ucuaccaggc cuucagcaag     1620

auccuggaga ucuccgggca cgagagcauc aacuucaaga aguccgcgga cgagaucgug     1680

gagcagguca agaccaucuu cagcgcccug ggcuggaaca ccgaggugcu cugcuucgac     1740

uccgagaagg agcuggacaa gcagcccuac uucaagcugu ggcaccuccu guacagcuuc     1800

gagggggacu ccacgcccac cggcgacggg aaccugaucc agaagaucgc cgaccucugc     1860

ggcuucgaga aggacuacgc cagcauccug gccaacauca ccuuccagga ggacuacggc     1920

agccugucca ccaaggcgau ccggaagauc cucccccacc ugaaggacgg gaaccaguac     1980

gacguggccu gcgaguacgc cggcuacaag cacagcaagu ccagccugaa gaaggaggag     2040

aucaagaaca aggugcucaa ggacaagcug gagcugcucc cgaagaacuc ccugcgcaac     2100

cccgucgugg agaagauccu gaaccagaug gugaacguca ucaacgccau caucacgacc     2160

uacgggaagc ccgacgagau ccggaucgag cucgcgcgcg agcugaagaa gaacgccaag     2220

gagcgcgagg agcugaccaa gagcaucgug gacaccacga agacccacga gggcauccgg     2280

gccauccucc agaacgacuu cgggaugucc cacgugagcc gcaacgccau cauccgguac     2340

aagcuguacg aggagcugaa ggacaacggc uacaagaccc ucuacuccaa caccuacauc     2400

cccaaggaga agcuguucag caaggacuuc gacaucgagc acaucauccc gcaggcccgc     2460

cuguucgacg acuccuucag caacaagacg cucgagguca aguccaucaa caucgagaag     2520

ggcagcaaga ccgcguacga cuucguggag gagaaguacg gggagcaggg ccugcaggag     2580

uaccugaacc ggugcgaggu ccucuuccgc gacaagaaga ccaagcugcg gaagcugaag     2640

augcaggagu ccgagauccc cgagggguuc aucgaccgcg accuccggaa cacccaguac     2700

aucgccaaga aggcccugag caugcugaac gagaucugcc gccggguggu ggccaccacg     2760

ggcgccauca ccgacgagcu ccgcgaggac uggcagcugg uggacgucau gaaggagcug     2820

aacugggaga aguacaaggu gcucggccug guggaguacu ucgaggacaa cgacgggcgg     2880

cugaucggcc gcaucaagga cuggaccaag cggaacgacc accgccacca cgcgauggac     2940

gcccucaccg ucgccuucac gaaggacgug uucauccagu acuucaacaa caagaacgcc     3000

ucccugaagc ccgacaccaa cgaggccgcg auccggaaca aguacuucca gaacgggaag     3060

gccaucccgc ccaugagccu ggacgaguuc cgcgccgagg ccaagaagca ccucaacgac     3120

acccugauca gcaucaagac caagaacaag guggucacga ucaaccugaa ccggaccaag     3180

aagaagaacg gcaucaacaa gaagcagcag cagacgcccc gcgggcagcu ccaccuggag     3240

accguguacg gcucccacaa gcaguacgug gucaaggagg agaaggugaa cgcgagcuuc     3300

gacgccgcca agaucgccac ggugaacaag cccgccuacc gggacgcgcu gcuccgccgc     3360

cugcaggaga acggcaacga cuccaagaag gccuucaccg ggaagaacag ccuggacaag     3420

cagcccgucu ggcucgacgc caagcagaac gagugcgugc cggagaaggu gaagaccguc     3480

augcuggaga ccguguacac ggugcggaag cccaucgacg agaagcugaa cgucgacaag     3540

gugguggacg cccgcauccg gaagcuccug caggaccgca ucaaggagua cggcaacgcc     3600

aaguccgcgu ucgccaaccu ggacgagaac cccaucuggc ucaacaagga gaaggggauc     3660

accaucaagc gggucagcgu guccggcauc agcaaggccc agucccugca cgagaagaag     3720

gacaaggacg ggaacuucau ccuggacaag gacagccgcc ggauccccgu ggacuucguc     3780

aacaccggca acaaccacca cguggccauc uaccgcaagc cggugcucga caagaagggc     3840

caggccgucu ucgacgagaa cggggagcug aaguucgagc uggaggaggu gguggucucc     3900

uucuucgacg cggugacccg ggugaaccuc agccagccca ucaucgacaa ggacuacaag     3960

cgcuccgagg gcuggaaguu ccuguucagc augaagcaga acgaguacuu cgucuucccc     4020

aacgagaaga cgggguucga cccgaaggag aucgaccugc ucaaccccga gaacuacgcc     4080

cugaucuccc ccaaccuguu ccgggugcag aagaucgcca gcaaggacua cgucuuccgc     4140

caccaccucg agaccguggu ggccgaccgg aaggagcugc aggagaucac cuggaagcgc     4200

aucuccagcc uguccauccu caaccggauc aucaaggucc gcgugaacca caucggccag     4260

aucgugauga ucggggagua cuga                                            4284


<210>  2180
<211>  4284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2180
auggccaagc ccaaggacua caccaucggc cuggacaucg ggaccaacag cgugggcugg       60

gucgugacgg acgaccagaa caacauccuc cgcaucaagg ggaagaaggc caucggcgcg      120

cggcuguucg ccgaggggaa gguggccgcc gagcgccggu ccuuccgcac cacccggcgc      180

cggcugagcc gccggcgcug gcggaucaag cuccuggagg agcuguucga caaggagauc      240

gccaaggucg acccguccuu cuucgcgcgc cuccacgaga gcuggaucuc ccccaaggac      300

aagcggaagc gcuacagcgc caucguguuc cccucccccg aggaggacaa gaaguuccac      360

gagagcuacc cgaccaucua ccaccuccgg gacaagcuga ugaaggacga ccagaagcac      420

gacauccgcg agaucuacau cgccgugcac cagaugauca aggcccgggg caacuuccuc      480

cacgacgagu ccgucgagac guaccgcagc ggcaugucca gccucggggg ccgguccgag      540

cgcaccaucc ugagcgugca gacccuggag gagcugaacg accuguucgc ggagaacgag      600

gggaccgagg aggccaagcu caacguggcc uccgccgagc agaucaacga cauccugacg      660

ggcgggcacc ugaacgccga cagccagaag gagaucggca accuccugcu gcccuccagc      720

uuccccuccu ucgacgacaa ggcgaaggag aagcagguca agaagcucau caagaacgug      780

gccaccaaca ucagcaaggc cuggcugggc uacaaggccg acuucagcac cauccugaac      840

cucgccaacg uggacaagga ccagaagaag aucuucgcgu ucgcccugca gggcggggac      900

gaggaggaca agguccagga gcuggagucc cuccuggagc agagccagac cgacaucgug      960

gaccggcuga ucgagauccg ccacgccauc gugcucuccg agaucguccc cguggggaug     1020

acgcugagcg aggccaugau cgacaaguac gaccagcaca aggaggaccu gaucacccuc     1080

aaggccguga uccgcaacac caaggacaag aagaaggcgg ccaagcugca ggccaucuac     1140

gaccuguacg ucaacaaccg gcacgccgac cucgccaagg cgaugaagcu gaccggcauc     1200

aagaagcgcu ccgagcugcu cgacccggag gagcugaaga aggccaucag cucccugcuc     1260

gacgacagcc ccgaggccgu ggagaucaag cagaugcugg aggagaagac guuccugccc     1320

cuccagcggu ccaacaacaa cggcgugauc cccaaccagc ugcaccaggu cgagcuggac     1380

gagaucauca agaagcagag caaguacuac ccguuccucg ccgagaagaa ccccgacgag     1440

uccgaggagg cgcagaagaa ggcccccacc aagcuggacg cccugcugac cuuccgcgug     1500

cccuacuacg uggggccgau gaucaccaag gaggagcagg aggcccagga cggccacagc     1560

uucgccugga ugguccggcg cgacccccag gaccacgagg cgaucacgcc cuggaacuuc     1620

gagaagaagg uggacaagau ggccuccgcc acccaguuca ucaagcggau gaccaccaag     1680

gacacguacc uccuggggga ggacguccug cccgccagcu cccucaagua ccagcuguuc     1740

accgugcuga acgagcucaa caaccugcgc gugaacggca agaagcugac cagcgacgag     1800

aaggagcagg ucaucgaggg gcuguucaag aagcagaaga ccgugaaggc cgacaaguuc     1860

gugaaguacu ggcaggcgaa gcacaucggc gccgacauca aggucaaggg ccuguccgac     1920

ccgagcaagu ucaacuccac gaugagcacc uacaucgacu ucaagaagau cuucggggac     1980

cagcugaacg acgugaaccg gcagaacgac uucgagaaga ucaucgagua cagcaccauc     2040

uucgaggacc gcaagauccu cgaggacaag cugcgggccg acuucgccug gcuguccgag     2100

gagcagauca agcagcucug ccgccugcgg cugcagggcu gggggcgccu cagcgacaag     2160

cugcugaccc ggcucacgga cgccgacggc cagaacgugc uggagcgccu guggaacucc     2220

aacgacaacu ucguccagau cgugagcgac cccaacauca aggcgaagau cgagcaggag     2280

aaccagaagc uccugcggga cgggcagucc gacgacgccg uggagagcau ccuggacgac     2340

gccuacacca gcccccagaa caagaaggcc auccgccagg ucaugcgggu ggugcacgac     2400

aucgucaagg cgugcgacgg caacgugccc gccaaguucg ccaucgaguu cgcccgcagc     2460

caggaggacg acccgaagcg caccaccgcc cgggugaacc agaugcagaa gaucuacgac     2520

cagaucuccg acgaggucgu gagccagggc gugaaggagc agcucuccgg gaugaagucc     2580

cugaaggacc gcuacuaccu guacuucaug cagggcgggc gggacgcgua cacgggcgcc     2640

aagcucaaca ucgaccgccu guccgacuac gacaucgacc acaucaugcc ccagagcuuc     2700

gucaaggacg acucccugga caaccgggug cucguggccc gcgccgucaa caaccagaag     2760

uccgacaagg ugcccgcccu gcuguucggg aacaaggugg ucgcggaccu cggcaucacc     2820

gugcgggaga ugugggacaa guggcagaag cugggcauga ucagcaagcg caagcugagc     2880

aaccuccuga ccgaccccga cgcccugacc gaguaccggg cccagggguu cauccgccgg     2940

cagcucgugg agaccagcca ggucaucaag cugacggcca ccauccugca guccgaguuc     3000

ccggacagca agaucaucga ggugcccgcc aaguacaacu ccaucgugcg caagcaguuc     3060

gaccucuaca agagccggga ggucaacgac uaccaccacg cgaucgacgc cuaccugucc     3120

accaucgugg gcaacuaccu guaccaggug uaccccaacc uccgccggau guucgucuac     3180

ggggaguuca agaaguucag cuccaacgcc gaggagagcg ccuacgacgu ggugcgccgg     3240

gucaagucca ugaacuuccu ggacgaccug cuccgcggca cccacggcga caacaucuac     3300

ugccggagca cgggggagau cguguucaac cgcaacgaca ucaucuccaa gcugaagcag     3360

gcguacagcu ucaagcagau gcuggugacc caggaggucu ucaccaagaa guccgcccuc     3420

uucgaccaga ccguguaccc gagccccgag cgggacucca agaagcgcag cggccugauc     3480

ccccgcaaga aggggaugga cacggagauc uacggcgggu acuccggcaa caaggacgcc     3540

uacuucgugc uggccgaggc cgucaaggag aagggccaca cccuccagau cgugggggug     3600

cccauccggg cgcugaacac ccugaagaac agcgccaacu acuccgagaa gcuccuggag     3660

aucaucaagc cgcaggucau guucaacaag gacaccggca agcccaucaa ggggaucaag     3720

gacgugaaga uccugaugga caagaucccc ugccgccagc ccgugcucga gggcgagagc     3780

uacuacaugc uggccagcuc caaguaccgg uacagccuga agcagaucuc ccucagccag     3840

auguccauga aguacauccu ggacuacauc gacgacccga acuucaacaa gcacgagaug     3900

aucaacaucg accagcagga cgagaaggag ugccugcuca gcgucuacga cgagauccug     3960

gagaagaugg acaaguaccu gccccucuuc gacauccgcu ccuuccggaa gaagcugcac     4020

gacgggcgcg acgccuucau cgcccugccc guggcgagcg aggagaagaa gcccggcaag     4080

gucgacguga uccggaaggu gcucaagggc cugcacgcca acgccgacau cacgaaccug     4140

gccgagcucg gguucggcac cgccgcgcug ggggcccugg ucuccaccgg cgggaucaag     4200

aucagcgacg acgccguguu caucuaccag uccccgaccg gccucuucga gcgccgggug     4260

aaggucagcg accugcugaa guga                                            4284


<210>  2181
<211>  4287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2181
augaagaaga uccugggccu cgaccugggg accaccagca ucggcuccgc cuacgugcug       60

gaggccgaga aguccgcgga gcagacgaag aucgucuacc ucgggacccg caucauccug      120

agcagcgccg acgccgagaa cuucuccaag ggccugagcu gcucccccac caccgaccgg      180

acguucaagc gcagcaugcg gcgccggcuc cagcgcuaca agcagcggcg cuccagccug      240

auccagcagu ucaagaagau cgggaucauc accaacaccg agcagaucau ccgggagccg      300

aucuccgaga cccacgagcu gcuccgcauc cggagcaagg ccguguccga gcgcgugagc      360

cuggaggagu ucgcccggau ccugcucacg aucaacaagc gccggggcua caaguccaac      420

cgcaagacca gcaaguccac cgaggaggac ggcaagcugg ucgacgcgau ggccgugacc      480

aaggagcuga ucaagcucaa gaagacgccc gggcaguaca uguucgagcg guucaagcgc      540

ggcaaguacg ccgagaucga cuucuacccc uucgaccuga ucaacgagcu gaagcagauc      600

ugggaggugc aggggcagaa guaccccgac guccucaccg aggaccugcu gagcucccuc      660

cugaagcaga agaccggcaa ggccaaggag cugaucuaca gcaagagcga ccucaacccg      720

gcggaggugc ccaucgacua ccagaagggg aagaagcugc ggaagaccau ccugaacaag      780

uacuucgccu acgagucccg cagccucgcc gccacgaaca ucauccccgu ggagcagguc      840

aucaccgcca ucguggcgau cugcaagcag aucaacaacu ccagcggcua ccugggcgcc      900

aucggggacc ggucccgcgu gcuguacgcc aacaaccaga ccaucggcca guaccucuac      960

gagcgcauca aggccaaccc ccacgaccgg auccgcgggg aggucuucua ccggcaggac     1020

uaccuggacg aguucgaccg caucugggag ugccagcaga acuacuaccc ggccauccug     1080

accaacgacc ggaagcaguu cauccgcaac cggaucaucu ucuaccagcg cccccucaag     1140

agcaagaagu ccgagcuggc guucugcgag uucgagagcc gggaggugga gcacaacggc     1200

aagaaguaca cggagggccu gcacguggcc cccaagucca gccccgucuu ccagcacuuc     1260

cgccucuggc agcggcuggg gcagaucgag guguccccgc ugcgcaagug gaacaagcag     1320

agcaacgagc uguucgagca gcagaacgac accgaccagg agcggauccu gaacaccgag     1380

gccaagcaga agcuguucuc cuaccucaac cugcuggacc accucaccga caacgagauc     1440

cugaacaucc ugggcuacag ccccgacaag uggaagauca acuuccgcga gaucaucggg     1500

aaccggacga acaacuccau gaugaaggcc uucuucaaca ugaaggagau gaccgacggc     1560

gagcagauca acugggagaa gaaccucacc aagaucacca acgggcagag ccugaucucc     1620

cccgagcaga ucguggaccu gauccgccag uucuucgagc gggagggcau cacgaccagc     1680

cugcucuacu ucgaccccac caccgacaac uuccgcgagc agucccacua cagccugugg     1740

caccuccucu acagcuacga ggacgacaac uccccgacgg gcaccaccag ccugugcaag     1800

acccuggcca agcgguacgg gaucccccac gagcucugcc cccugcuggc gucccucacg     1860

uucgagcccc aguacggcag ccuguccacc aaggccaugc gcaagauccu gccguucaug     1920

caggagaacg gguacgacaa ggccugcgag cucgccggcu accgccacag cgccuggacc     1980

accaaggagg agaacaccga gcggacgcug aagaacuccc ugccccuccu gcgcaagggg     2040

agccugcgga accccgucgu ggagaagauc cucaaccaga ugacccacgu gaucaacacc     2100

cacaucuccg aguacggcaa ccccgacgag auccgcaucg agauggcgcg ggacaugcag     2160

gccaacagca agcagcgcaa ggccaugacc acggccaucg cggaggccac caagcggaac     2220

acccaguuca ucgaggagau ccagaaggag uucggcuuca acccguccaa gaacgaccug     2280

aucaaguaca agcuguggaa ggagcucgcg ccccugggga gcaagacccu guacuccaac     2340

acguacaucc ccaaggagga gcuguucacc gccaaguucg agaucgagca caucaucccc     2400

cgcgcccugu ucuacgacga cagcuucucc aacaagaccc uggagcucaa cagcgucaac     2460

aagcugaagg gcaacaagac cgcccuggac uacauggaca cgcucuccca ggagcaggcc     2520

aacgacuaca ucauccgggu ggaggagcug aagaagaucg gggccaucac ccaggccaag     2580

uacaagaagc ugcucauccg cgagaaggac cugggcgacg acuucaccaa ccgggaccug     2640

accaacacgc gcuacaucau ccggcacgcc gcggacaugc uccuccaggu gaccccgagc     2700

aucguccaca ccaacgggag caucaccgcc auccugcgcc gggacuggga gcuccuggac     2760

auccugaagg agcucaaccu gcccacguac gaguccaucg accgcaagau cagcgacccc     2820

aaggagaagc ugaccgaguc cguggagcgg aaggacggcc aggccaucaa gaugaucaag     2880

gacuggagca agcgcgacga ccaccggcac cacgccaugg acgcccucau caccgcgcug     2940

accacgcgca agcacgucca guaccugaac ggcaaccgcg gggccgagga gaccgacaag     3000

gugcagaagc uccagcagcu gaaggcccug cggaagcagc ucaucggcuc cgacgggcac     3060

uucaucccgc ccaugcacga cauccgcgug caggccaagg aggcgcugag ccgggucauc     3120

aucuccacca aggccaaggg caaggugacc acgcccaaca ccaacaucau cugcagccgc     3180

gacaagacca ccggccggaa gaucuacgug aagcagacga cccugacgcc ccgcggggcc     3240

cuccacaagg agaugcagua cggcugcaac cacuacuacc uggagaagca gaucccgauc     3300

ggguccaagc ugaccgagga ggagauccgg cucgucagcu cccccaagau ccgcagcaug     3360

cuguccgagc ggcugcaggc cuacaacaac gaccccaaga gcgccuucac cggccgcaac     3420

uccauggaca agaagccccu cgaggacaag aacaagaagc ugguguacac cgacaagaag     3480

gugaagcaga ccauccuggc gaaccgguuc gucaagcgcg accgggugaa cagcaagcuc     3540

gaccuggaca aggugaucga caccgggauc aagcgcaucc uggagcggcg ccucaagcag     3600

uacggcggca aguacgagac cgccuucucc gaccuggaga acaacccgau cuggcugaac     3660

gaggagaagg ggaucgccau caagagcguc cggauccgcg gcaagaacaa cacgaucacc     3720

auccggcaga ccaacacgug ccgcaucaag uacguggcca acgacaacaa ccaccaccuc     3780

gccguguacg cgcugcccga cggguccauc gaggacgaca ucgucagcuu cuucgacgcc     3840

guggagcgga gccgcaaggg ccugcccauc aucgacaagc acaagaacca gucccucggg     3900

uggaaguucc uguucagcgu gaagcagaac gaguacuucc ugcuccccca gcagggcgag     3960

aagaucgaca ucgacaccgg cgagaucucc augcagacca ccuucagccc gaaggaccug     4020

ucccccgagu acaucaugaa ccccgacaac uacgccgagc uggcgcccca caucuuccgc     4080

guccagagca ucuccaagac cccgcagggg aaccgggacg ugcgcaucua ccucuucaag     4140

cagcaguaca ucagcggcuc ccagaaggac agccugcacc ugaaggggau cacgcaccgg     4200

cagaucucca gcgccaagga ccucgccggc cuggugaaga uccgcgucaa ccaccugggg     4260

cggaucgugg ccgugggcga gaaguga                                         4287


<210>  2182
<211>  4287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2182
augaagaaga uccugggccu cgaccugggg accgccagca ucggcuccgc cuacgugcug       60

gaggcggaga aguccgccga ccagaccaag aucgucuacc ucgggacgcg caucauccug      120

uccagcgccg acgccgagaa cuucuccaag ggccugagcu gcucccccac caccgaccgg      180

accuucaagc gcagcaugcg gcgccggcuc cagcgcuaca agcagcggcg cuccagccug      240

auccagcagu ucaagaagau cgggaucauc acgaacaccg agcagaucau ccgggagccg      300

aucuccgaga cccacgagcu gcuccgcauc cggagcaagg ccguguccga gcgcgugagc      360

cuggaggagu ucgcgcggau ccugcucacc aucaacaagc gccggggcua caaguccaac      420

cgcaagacga gcaaguccac cgaggaggac ggcaagcugg ucgacgccau ggccgugacc      480

aaggagcuga ucaagcucaa gaagaccccc gggcaguaca uguucgagcg guucaagcgc      540

ggcaaguacg ccgagaucga cuucuacccc uucgaccuga ucaacgagcu gaagcagauc      600

ugggaggugc aggggcagaa guaccccgac guccucacgg aggaccugcu gagcucccuc      660

cugaagcaga agaccggcaa ggcgaaggag cugaucuaca gcaagagcga ccucaacccg      720

gccgaggugc ccaucgacua ccagaagggg aagaagcugc ggaagaccau ccugaacaag      780

uacuucgccu acgagucccg cagccucgcc gugaccaaca ucauccccgu cgagcaggcc      840

aucacggcga ucguggccau cugcaagcag aucaacaacu ccagcggcua ccugggcgcc      900

aucggggacc ggucccgcgu gcuguacgcc aacaaccaga ccaucggcca guaccucuac      960

gagcgcauca aggccaaccc ccacgaccgg auccgcgggg aggucuucua ccggcaggac     1020

uaccuggacg aguucgaccg caucugggag ugccagcaga acuacuaccc ggcgauccug     1080

accaacgacc ggaagcagua cauccgcaac cggaucaucu ucuaccagcg cccccucaag     1140

agcaagaagu ccgagcuggc cuucugcgag uucgagagcc gggaggugga gcacaacggc     1200

aagaaguaca ccgagggccu gcacguggcc cccaagucca gccccgucuu ccagcacuuc     1260

cgccucuggc agcggcuggg gcagaucgag guguccccgc ugcgcaagug ggacaagcag     1320

agcaacgagc uguucgagca gcagaacaac gccgaccggg agggcauccu gaacacggag     1380

gccaagcaga agcuguucuc cuaccucaac cugcuggacc accucaccga caacgagauc     1440

cugaacaucc ugggguacag ccccgacaag uggaagauca acuuccgcga gaucaucggc     1500

aaccggacca acaaccucau gaugaaggcg uucuucucca ugaaggagau gaccgacggg     1560

gagcagaucg acugggagaa gaaccugacg aagaucacca acggccagag ccugaucucc     1620

cccgagcaga ucguggaccu gauccgccag uucuucgagc gggagggcau caccaccagc     1680

cuccuguacu ucgaccccgc cacggacaac uuccgcgagc agucccacua cagccugugg     1740

caccuccugu acagcuacga ggacgacaac uccccgaccg ggaccaccag ccugugcaag     1800

acgcucgcca agcgguacgg caucccccac gagcugugcc cccugcucgc cucccugacc     1860

uucgagcgcc aguacgggag ccuguccacc aaggccaugc gcaagcuccu gcccuucaug     1920

caggagaagc gguacgacga ggcgugcgag cuggccggcu accgccacag cgccuggauc     1980

accaaggagg agaacgccaa gcggacgcuc aagaacaagc ugccgcugcu ccgcaagggg     2040

ucccugcgga accccgucgu ggagaagauc cugaaccaga ugacccacgu gaucaacacc     2100

cacaucgcgg aguacggcaa ccccgacgag auccgcaucg agauggcccg ggacaugcag     2160

gccaacagca agcagcgcaa ggccaugacc gccgcgauca ccgaggccac gaagcggaac     2220

acccaguuca ucgaggagau ccagaaggag uucggcuuca accccuccaa gaacgaccuc     2280

aucaaguaca agcuguggaa ggagcuggcc ccgcucggga acaagacccu guacagcaac     2340

aucuacaucc ccaaggagga gcuguucacc gccaaguucg agaucgagca caucaucccc     2400

cgcgcccucu ucuacgacga cuccuucagc aacaagacgc uggagcugaa cuccgucaac     2460

aagcucaagg gcaacaagac cgcgcuggac uaccuggaca cccucgggca ggagaaggcc     2520

aacgacuaca uccugcgggu ggaggagcug aagaagaucg gcgccaucac ccaggccaag     2580

uacaagaagc uccugauccg cgagaaggac cugggggacg acuucacgaa ccgggaccuc     2640

accaacaccc gcuacaucau ccggcacgcc gcggacaugc ugcuccaggu gacccccagc     2700

aucguccaca cgaacggcuc caucaccgcc auccuccgcc gggacugggg ccugcuggac     2760

auccucaagg agcugaaccu gccgaccuac gagagcaucg accgcaagau cuccgacccc     2820

gaggagaagc ucacccagag cguggaccgg aaggacgggc aggccaucaa gaugaucaag     2880

gacuggagca agcgcgacga ccaccggcac cacgccaugg acgcgcugau cacggcccug     2940

accacccgca agcacgucca guaccucaac ggcaacuccg gggccgagga gaccgacaag     3000

gcccagaagc uggagcagcu gaaggcccuc cgcaagcagc ugaucggcag cgacggccac     3060

uucaucccgc ccaugcacga cauccgggug caggcgaagg aggcccuguc ccgcgugauc     3120

aucagcacga aggucaaggg gaaggugacc acccccaaca ccaacaucau cugcucccgg     3180

gacaagacga ccggccgcaa gaucuacgug cggcagacca cccucacgcc ccgcggggcc     3240

cugcaccugg acaccaagua cggcaucaac cgguacuacc aggagcgcga ggucagcauc     3300

ggguccaagc ucacccagga ggagauccgg cuggugagca gcccccacau ccagagcauc     3360

cugcucgagc gccugcgggc cuucgacaac aacccgaagu ccgccuucac cggccgcaac     3420

agcguggaca agaagccccu ggaggacaag aacaagaagc ucgucuacac ggacaagaag     3480

gugaagcaga ccauccuggc gaaccgguuc gugaagcgcg accaggucaa cuccaagcug     3540

aaccucgaca aggugaucga caccggcauc aagcggaucc uggaggggcg ccugcagcag     3600

uacggcggga aguacgagac cgccuucagc gaccucgaga acaaccccau cuggcugaac     3660

gaggagaagg gcaucgccau caaguccgug cggauccgcg ggaagaacaa cgccaucccc     3720

auccaccaga cggacacgug cccgaucgac uacgucgcca acgacaacaa ccaccaccug     3780

gcgguguacg ugcuccccga cggcaccauc gaggacgaca ucgucaccuu cuucgacgcc     3840

guggagcgga gccgccaggg ccugcccauc guggacaaga acaagaacca gucccugggg     3900

uggaaguucc ucuucagcau caagcagaac gaguacuucc ugcugcccca gcaggccgag     3960

aagaucgaca ucgacaccgg cgagaucucc acccagacca ccuucagccc gaaggaccuc     4020

agccccgagu acaucaugaa ccccgacaac uacgccgagc uggcguccca caucuuccgc     4080

guccagagca ucuccaagac gccccagggg aaccgggacg ugcgcaucua ccuguucaag     4140

cagcaguaca ucagcggcuc ccagaaggac agccuccacc ugaaggggau caccuaccgg     4200

cagaucucca gcgccaagga ccuggccgag cucgugaaga uccgcgucaa ccaccugggc     4260

cggaucgugu ccgugggcga gaaguga                                         4287


<210>  2183
<211>  4287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2183
augaccaaga agauccuggg ccucgaccug gggaccacga gcaucggcug ggccuacgug       60

aucgaguccg acaaccccga cgagagcgag aucaagcaga ucgggguccg cgugaacccg      120

cugaccaccg acgagcagac caacuucgag aagggcaagc ccuucuccau caacaaggag      180

cggacgcuca agcgcggggc ccggcgcaac cuggaccggu acaaggaccg ccggaacaac      240

cugaucgagg ugcucaagaa ggcgaacauc cugagccagg acuccauccu gaccgaggag      300

ggccagcaca ccacccacca gacguggcag cuccgcgcca aggccgccgu cgacaagauc      360

gagaaggagg agcuggcccg ggugcugcuc gcgaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagaacga ggacgagggg caggccaucg acggcauggc cguggcgaag      480

cgccuguacg acgagcagau cacgugcggg cagcuggucu accagcuccu gcgggagggc      540

aagaagucca uccccgacuu cuaccgcucc gaccugcagg ccgaguucga caaggugugg      600

aacuuccaga agcaguucua ccccaagauc cucaccgacg aguucuacaa ggagcugaag      660

gggaagggcc agcgggccac cagcgcccug uucuacaaga ccuacaacuu caacaccgcc      720

gagaacaagg gcucccgcga ggagaagaag auccaggcgu acaaguggcg gagcgaggcc      780

aucucccagc agcucgagaa ggaggaggug gccuucgucc ugaccgagau caacaacaac      840

cugaacaaca gcuccgggua ccucggcgcc aucagcgacc gcuccaagga gcuguacuuc      900

aacaagcaga ccguggggca guaccuguac cagcagcucc aggagaaccg gcacgcccgc      960

cugaagaacc agguguucua ccggcaggac uaccuggacg aguucgaggc gaucuggaag     1020

gagcaggcca agcaccacac ggagcucacc gaggagcuga aggccgagau ccgcgacauc     1080

aucaucuucu accagcgcaa gcucaagagc cagaagggcc ugaucuccuu cugcgaguuc     1140

gagagccggg agauccagau cgacggcaag aagaagacca ucgggcugcg cgucgccccc     1200

aagagcagcc cccuguucca ggaguucaag aucuggcaga accucaacaa cgugcuggug     1260

cggaagaagg gcagccgcaa gcggaacguc aagcaggaga uccagggguc ccuguucgag     1320

gaggagaagg agaucuucgu guucgaccuc gagaccaagg agaagcuguu cgaggagcug     1380

aaccucaagg gcaaccugag cgccaacaag gugaucgagc ugcucgggca caagcccacg     1440

gagugggaga ucaacuacuc cgcgcuggag ggcaaccgca ccaaccaggc ccuguacaac     1500

gccuaccuca agauccugga caucgagggc uacgacgagg accugcugaa gcucagcgac     1560

aaggacgaca ucaacgucgc cgaccugaag acccccgccu ccgagaucaa gaacauggug     1620

cgggacaucu uccaggugcu ggggaucagc accgacaucc ucgaguucga cgcggagcug     1680

gacggcaagg acuucgagca gcaggccucc uaccagcugu ggcaccuccu guacagcuac     1740

gagggggacg acucccagag cggcaacgag aagcuguacg agcuccugga gaagaaguuc     1800

ggguucaaga aggagcacuc ccagguccug gccaacguga cgcucagcga cgacuacggc     1860

aaccugucca gcaaggccau gcgcaagauc uacccguuca ucaaggagaa caacuacucc     1920

accgcgugcg agcuggccgg cuaccggcac agcaagcacu cccucaccaa ggaggagauc     1980

gagaaccgcc cccuggacga caagcuggag cuccugaaga agaacagccu gcggcagccc     2040

guggucgaga agauccucaa ccagauggug aacgucguga acagccugcu ggagaagaac     2100

cgcaaggaga accccgacuu ccgguucgac gagauccgca ucgagcucgc ccgggagcug     2160

aagaagaacg ccaaggagcg cgccgagaug accacgaaca ucaacuccgc gaagaccgag     2220

aacgagaaga ucuucaagcu gcuccaggcc gaguucgggc ugaagaaccc gacccggaac     2280

gacaucaucc gcuaccggcu guacgaggag cucaagaaca acggcuacaa ggaccuguac     2340

accaacaagu acaucccccg cgagauccug uucagcaagc aggucgacau cgagcacauc     2400

aucccccagu cccgccucuu cgacgacagc uucuccaaca agacgaucgu guaccgggag     2460

accaaccugg acaaggggaa ccagaccgcc uucgacuaca ucgacagcaa guacggcaac     2520

gagaaggugg aggaguacau ccugcgcauc gaggcccucc acgacgccug gaagaagaac     2580

aaggaggagg ggaucuccaa ggcgaaguac cagaagcugc ugaagaagga gaccgagauc     2640

ggcgacggcu ucaucgagcg ggaccuccgc gagagccagu acaucgccaa gaaggccaag     2700

gagaugcugu ucaagaucac gcgguccgug gucagcaccu ccgggagcau caccgacaag     2760

cugcgcgagg acuggggccu caucaacauc augaaggagc ugaaccugcc caaguaccgg     2820

ucccucgggc ugaccgagau gcaggagcgc aaguucggcc agaaggugga ggugaucaac     2880

gacuggacca agcggaacga ccaccgccac cacgccaugg acgcccugac ggucgcguuc     2940

accaagcaca accacaucca guaccucaac uaccugaacg cccggaagaa cgagaaccac     3000

aagcugcaca acaacaucau cgccaucgag aaccucgaga ccgagaccau gaucgacaac     3060

aacggcagca agcgccgggu guucaaggcc ccgaugccca acuuccgcca gguggcgaag     3120

gagcaccugg agaacguccu gaucucccac aaggccaaga acaagguggu gacgaagaac     3180

aagaacaaga ucgccgggaa ggucgccgcc caggagaccc ucaccccgcg gggccagcug     3240

cacaaggaga ccguguacgg gaaguaccag uacuacguga acaaggagga gaagaucggc     3300

acgaaguucg acgaggagac caucaacaag gucagcaacc cccaguaccg caagcugcuc     3360

cuggagcggc uguccgcgaa cgggaacgac ccgaagaagg ccuucagcgg caagaacgcc     3420

cucagcaaga accccaucua ccugaacgag accaagaccg agacggugcc cgaggugguc     3480

aagcugaccu ggcucgagga ggacuacucc auccgcaagg acaucacccc cgacaacuuc     3540

aaggacgaga aguccaucga caagauccug gacgagggcg ugaagcggau ccugaagaag     3600

cgccuccgga agaacgacaa cgacccgaag aaggccuuca gcgaccugga caagaacccc     3660

aucuggcuga acgaggagaa ggggaucagc aucaagcgcg ugaccaucuc cggcgucaac     3720

aacgccgagc cccuccacua caagaaggac cacuucggga acgacauccu ggacgagaag     3780

ggcgacaaga uccccgugga cuucgugagc acggggaaca accaccacgu cgcgguguac     3840

cgcgacgaga agggcaaccu gcaggagaac aucguguccu ucuucgaggc cgucauccgg     3900

gugaaccagg gccucccgau caucgacaag gccuacaacc agcaccuggg guggcaguuc     3960

cuguucacca ugaagcagaa cgagauguuc guguucccca acgagaaguc cggcuucaac     4020

ccccaggaga ucgaccuccu ggacccgagc aacaagaaga agaucagccc caaccuguuc     4080

cgcguccaga agauguccaa ggugaucuac gggaacagcc ccgugcggga guacgucuuc     4140

cgccaccacc ucgagaccaa cguggacgag aagaaggagc ugaaggacau caccuucaag     4200

aacaucaagu cccugggcua cuucgaggac aucgugaagg uccggaucaa ccaccucggg     4260

gacaucguga gcgugggcga guacuga                                         4287


<210>  2184
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2184
augaagcaca uccugggccu cgaccugggg accaacagca ucggcugggc cguggucaag       60

gaggcccaga acgacaacga gcaguccgag aucguggacg uggggguccg cgugaacccc      120

cugaccacgg acgagaagac caacuucgag aagggcaagc cgaucagcac caacgcggac      180

cggacccuca agcgcuccgc ccggcgcaac cugcagcggu acaagcugcg ccggaaggag      240

cucaucgagc ugcugaagga gcaccagcuc aucagcacgg acaccauccu gaccgagaac      300

gggaagggca ccacguucca gacccuggag cuccgcgcca aguccgccaa ggagaagauc      360

agccuggagg agcuggcccg gguguuccuc accaucaaca agaagcgcgg cuacaagucc      420

agccggaagg cgaagaacga ggacgagggg cagcugaucg acggcaugga gaucgccaag      480

cgccuguacg aggagaaccu caccacgggg cagcuguccc ugcagcuccu gaaggagggc      540

aagaaguccc ugcccgacua cuaccggagc gaccuccagu ucgagcugga caaggucugg      600

aacuuccaga agcaguucca cagcgacauc cugaccgacg aguucuacgc ccagcucaag      660

gggaagggcc agcgcgccac cuccgcgcug uuccuggcca aguaccagau cuacaccgcc      720

gagaacaagg gcagccggga ggacaagaag cuccaggccu accaguggcg cguggacgcc      780

cuguccaagg agcugacgcg ggagcagcuc gcguucguga ucaccgagau caacaacaac      840

cugaacaaca gcuccgggua ccugggcgcc aucagcgacc gcuccaagga gcucuacuuc      900

aacaacgaga ccgucgggga guaccugugg aagcagaucg ccgccaaccc ccacaccagc      960

cugaagaacc agguguucua ccggcaggac uaccucgacg aguucgagca gaucuggaag     1020

acgcagagcg ccuucuaccc cgagauccug accgacgagc ugaagaagga gcuccgcgac     1080

guggucaucu ucuaccagcg gaagcugaag ucccagaagu cccuggugag cuucugcgag     1140

uucgagaaca aggagaucga gaucaagaag gaggacggca ccagcaccaa gaaggugauc     1200

ggccucaagg ucgcgccgaa guccagcccc cuguuccagg aguucaagau cuggcagaac     1260

cugaacaacg ugcucauccg caagaagggg uccaagacga agaagguggc caagaaccag     1320

caggccgucc uguucgagga ggagaagacc aucagcgagc uggaccugga ggccaagcag     1380

gcccucuuca acgagcugaa ccugaagggc aaccucaagg cggacuacug ccuggagauc     1440

cugggguacu cccccaagga gugggagaug aacuacagcg ugcucgaggg caaccgcacc     1500

aacaaggccc uguacgaggc cuaccugaag aucaccgacc ucgaggggua cgacgccaag     1560

aaccaccuga aggugaagcu gaacaaggac gaggucgagc ucgacgaccu gaaggugccc     1620

gccuccgaga ucaaggacau ggugcaggcg aucuucaagg aggccggcau caacacggac     1680

auccugcacu ucaacgccga gcucgacggc aaggacuucg agaaccaggc cagcuaccag     1740

cuguggcacc uccuguacuc cuacgagggg gacgacagca aguccggcaa cgagcugcug     1800

uacaagcucc uggagaccaa guucggguuc aagaaggagu acgcgcagau ccuggccaac     1860

gucagccucu ccgacgacua cggcagccug uccaccaagg ccaugcggaa gauccacccg     1920

uacaucaagg agaacaaguu cagcaccgcc ugcgagcugg ccggguaccg ccacagcaag     1980

cacucccuca cgaaggagga gaacgacaac cgggugcuga aggacagccu ggagguccuc     2040

aagaagaacu cccugcgcca gcccguggug gagaagaucc ugaaccagau gaucaaccuc     2100

gucaacgcgc ugcugaagaa guaccggacc gagaacccca acuucaaguu cgacgagauc     2160

cgcaucgagc ucgcccggga gcugaagaag aacgccgagg agcgcgccaa caugaccgcc     2220

aacaucaaca aggcgaagac cgagcacgag aagaucauca agauccugaa gaacgaguuc     2280

ggccucccca acccgacgcg gaacgacauc auccgcuacc ggcuguacga ggagcugaag     2340

gccaacggcu acaaggaccu cuacaccaac aucaagaucc cccaggagaa gcuguucacc     2400

aaggagaucg acaucgagca caucaucccc aagagccgcg uguucgacga cuccuucagc     2460

aacaagaccc uguccuuccg gaagaccaac cucgacaagg gggagcgcac ggccuucgac     2520

uacauggaga gcaaguucgg cgccgacaag cuggaggagu acguggcccg ggucgaggac     2580

cuguacaaga acaacaucau cuccaaggcg aaguaccaga agcuccugaa gaaggagagc     2640

gagaucgggg acggcuucgu ggagcgcgac cugcgggaga cccaguacau cgccaagaag     2700

gccaaggaga uccuccugca gaucaccccc aacgugcugu ccaccagcgg gggcaucacg     2760

gaccgccucc gggaggacug ggaccugauc aacgucauga aggagcugaa ccucccgaag     2820

uaccgcgccc ugggccugac ccagauggag gaccgcaagu acgggcagca ggucgaggug     2880

aucaccgacu ggaccaagcg gaacgaccac cgccaccacg cgauggacgc ccucacggug     2940

gccuucacca agcacaacca cauccaguac cugaacuacc ugaacgcccg gaagaacgag     3000

aagcacaagg agcacuccaa caucaugggc auccagcagc ucgagaccau caaggugacc     3060

gacaagaacg ggaacgagaa gcgcguguuc aaggccccca ugcccaacuu ccggcagguc     3120

acgaaggcgu uccuggagaa cauccugauc agccacaagg ccaagaacaa gguggugacc     3180

aagaacaaga acaaggucgc cggcuccaac aaggugcagg aggugcucac gccccgcggc     3240

cagcugcaca aggagaccgu cuacgggaag uaccaguacu acgugcagaa ggaggagaag     3300

aucggcgcca aguucgaccu ggagaccauc aagaaggugg ccaacccgcu cuaccagagc     3360

cugcugaaga agcggcucga ggagaacggg ggcgacccca agaaggcguu caccgggaag     3420

aacucccugg ccaagaaccc caucuaccug gacgacaccc agaccgagca gcuccccgag     3480

aaggucaagc ugguguggcu ggagaacagc uucagcaucc gcaaggacau cacgccggag     3540

aacuucaagg acgagaagcu caucgacaag auccuggacg agggcgugaa gcggauccug     3600

aaggagcgcc uccaguccuu cggcaacgac gccaagaagg ccuucagcga ccuggacaag     3660

aaccccaucu ggcugaacaa ggagaagggg aucaccauca agcgggucac caucuccggc     3720

guggccaacg cggagccccu ccacaccaag aaggaccacc uggggaagga gauccuggac     3780

aaggagggca agcccauccc gguggacuuc aucagcacgg gguccaacca ccacgucgcc     3840

guguaccgcg acgagaaggg caaccuccag gagaaggugg ucagcguguu cgaggccgug     3900

guccgggcca accagggccu gcccaucauc gacaagaccu acaaccagca ccuggggugg     3960

gaguuccucu ucaccaugaa gcagaacgag auguucgugu uccccaacga guccaccggc     4020

uucaacccga acgacaucga ccugcuggac cccaagaaca agcgcaucau cagccccaac     4080

cucuuccggg ugcagaaguu cuccaagcug caguacggga acagcuucgu ccgcgaguuc     4140

guguuccggc accaccugga gacgaccgug gaggaccgca aggagcugca gggcaucacc     4200

uacaagcaga ugaagaacuc caccuucuuc aacgacauca ucaagguccg gaucaaccac     4260

cucggggaca ucgugagcau cggcgaguac uga                                  4293


<210>  2185
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2185
augaagcaca uccugggccu cgaccugggg accaacagca ucggcugggc cguggucaag       60

gaggcccaga acgacaacga gcaguccgag aucguggacg uggggguccg cgugaacccc      120

cugaccacgg acgagaagac caacuucgag aagggcaagc cgaucagcac caacgcggac      180

cggacccuca agcgcuccgc ccggcgcaac cugcagcggu acaagcugcg ccggaaggag      240

cucaucgagc ugcugaagga gcaccagcuc aucagcacgg acaccauccu gaccgagaac      300

gggaagggca ccacguucca gacccuggag cuccgcgcca aguccgccaa ggagaagauc      360

agccuggagg agcuggcccg gguguuccuc accaucaaca agaagcgcgg cuacaagucc      420

agccggaagg cgaagaacga ggacgagggg cagcugaucg acggcaugga gaucgccaag      480

cgccuguacg aggagaaccu caccacgggg cagcuguccc ugcagcuccu gaaggagggc      540

aagaaguccc ugcccgacua cuaccggagc gaccuccagu ucgagcugga caaggucugg      600

aacuuccaga agcaguucca cagccacauc cugaccgacg aguucuacgc ccagcucaag      660

gggaagggcc agcgcgccac cuccgcgcug uuccuggcca aguaccagau cuacaccgcc      720

gagaacaagg gcagccggga ggacaagaag cuccaggccu accaguggcg cguggacgcc      780

cuguccaagg agcugacgcg ggagcagcuc gcguucguga ucaccgagau caacaacaac      840

cugaacaaca gcuccgggua ccugggcgcc aucagcgacc gcuccaagga gcucuacuuc      900

aacaacgaga ccgucgggga guaccugugg aagcagaucg ccgccaaccc ccacaccagc      960

cugaagaacc agguguucua ccggcaggac uaccucgacg aguucgagca gaucuggaag     1020

acgcagagcg ccuucuaccc cgagauccug accgacgagc ugaagaagga gcuccgcgac     1080

guggucaucu ucuaccagcg gaagcugaag ucccagaagu cccuggugag cuucugcgag     1140

uucgagaaca aggagaucga gaucaagaag gaggacggca ccagcaccaa gaaggugauc     1200

ggccucaagg ucgcgccgaa guccagcccc cuguuccagg aguucaagau cuggcagaac     1260

cugaacaacg ugcucauccg caagaagggg uccaagacga agaaggugga caagaaccag     1320

caggccgucc uguucgagga ggagaagacc aucuucgagc uggaccugga ggccaagcag     1380

gcccucuuca acgagcugaa ccugaagggc aaccucaagg ccuacuacug ccuggagauc     1440

cugggguaca gccccaagga gugggagaug aacuacuccg ugcucgaggg caaccgcacc     1500

aacaaggcgc uguacgaggc cuaccugaag aucaccgacc ucgaggggua cgacgccaag     1560

aaccaccuga aggugaagcu gaacaaggac gaggucgagc ucgacgaccu gaaggugccc     1620

gccagcgaga ucaaggacau ggugcaggcc aucuucaagg aggcgggcau caacacggac     1680

auccugcacu ucaacgccga gcucgacggc aaggacuucg agaaccaggc cuccuaccag     1740

cuguggcacc uccuguacag cuacgagggg gacgacucca agagcggcaa cgagcugcug     1800

uacaagcucc uggagaccaa guucggguuc aagaaggagu acgcccagau ccuggcgaac     1860

gucucccuca gcgacgacua cggcucccug agcaccaagg ccaugcggaa gauccacccg     1920

uacaucaagg agaacaaguu cuccaccgcc ugcgagcugg ccggguaccg ccacagcaag     1980

cacagccuca cgaaggagga gaacgacaac cgggugcuga aggacucccu ggagguccuc     2040

aagaagaaca gccugcgcca gcccguggug gagaagaucc ugaaccagau gaucaaccuc     2100

gucaacgccc ugcugaagaa guaccggacc gagaacccca acuucaaguu cgacgagauc     2160

cgcaucgagc ucgcgcggga gcugaagaag aacgccgagg agcgcgccaa caugaccgug     2220

aacaucaaca aggccaagac cgagcacgag aagaucauca agauccugaa gaacgaguuc     2280

ggccucccca acccgacgcg gaacgacauc auccgcuacc ggcuguacga ggagcugaag     2340

gccaacggcu acaaggaccu cuacaccaac gaguacaucg agaagcagga ccuguucacc     2400

aagaacaucg acaucgagca caucaucccc aagucccgcg uguucgacga cagcuucucc     2460

aacaagaccc ugagcuuccg gaagaccaac cucgacaagg gggagcgcac ggcguucgac     2520

uacaucgagu ccaaguucgg cgccgacaag cuggaggagu acgucgcccg gguggaggac     2580

cuguacaaga acaacaucau cagcaaggcc aaguaccaga agcuccugaa gaaggagucc     2640

gagaucgggg acggcuucgu ggagcgcgac cugcgggaga cccaguacau cgccaagaag     2700

gcgaaggaga uccuccugca gaucaccccc aacguccuga gcaccuccgg gggcaucacg     2760

gaccgccucc gggaggacug ggaccugauc aacgugauga aggagcugaa ccuccccaag     2820

uaccgcgccc ugggccugac ccagauggag gaccgcaagu acgggcagca ggucgaggug     2880

aucaucgacu ggaccaagcg gaacgaccac cgccaccacg ccauggacgc ccucaccgug     2940

gcguucacga agcacaacca cauccaguac cugaacuacc ugaacgcccg gaagaacgag     3000

aagcaccgcg agcacagcaa caucaugggc auccagcagc ucgagaccau caaggugacc     3060

gacaagaacg ggaacgagaa gcgggucuuc aaggccccga ugcccaacuu ccgccaggug     3120

accaaggccu uccuggagaa cgugcugauc ucccacaagg ccaagaacaa ggucgugacg     3180

aagaacaaga acaagguggc gggcagcaac aagguccagg aggugcucac gccccggggc     3240

cagcugcaca aggagaccgu guacgggaag uaccaguacu acguccagaa ggaggagaag     3300

aucggcggga aguucgaccu ggagaccauc aagaaggugg ccaacccccu cuaccagucc     3360

cugcugaaga agcgccucga ggagaacggc ggggacccga agaaggccuu caccggcaag     3420

aacagccugg ccaagaaccc caucuaccug gacgacgccc agaccgagca gcuccccgag     3480

aaggugaagc uggucuggcu ggagaacucc uucagcaucc ggaaggacau cacccccgag     3540

aacuucaagg acgagaagcu caucgacaag auccuggacg agggcgugaa gcgcauccug     3600

aaggagcggc uccagagcuu cgggaacgac gcgaagaagg ccuucuccga ccuggacaag     3660

aacccgaucu ggcugaacaa ggagaagggc aucgccauca agcgcguggc caucagcggg     3720

gucgccaacg cggagccccu ccacaccaag aaggaccacc ugggcaagga gauccuggac     3780

aaggagggga aguccauccc cguggacuuc aucagcacgg gcaacaacca ccacguggcc     3840

gucuaccggg acgagaaggg caaccuccag gagaaggugg uguccgucuu cgaggccgug     3900

gugcgcgcca accaggggcu gcccaucauc gacaagaccu acaaccagca ccugggcugg     3960

gaguuccucu ucaccaugaa gcagaacgag auguucgucu ucagcaacga guccaccggg     4020

uucaacccga acgacaucga ccugcuggac cccaagaaca agaagaucau cagccccaac     4080

cucuuccggg ugcagaaguu cuccaagcug caguacggca acagcuucgu gcgcgaguuc     4140

gucuuccggc accaccugga gacgaccgug gaggaccgca aggagcugca ggggaucacc     4200

uacaagcaga ugaagaacuc cgcguucuuc aacgacauca ucaaggugcg gaucaaccac     4260

cucggcggca ucgucagcgu gggggaguac uga                                  4293


<210>  2186
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2186
augaaggaca uccugggccu cgaccugggg accaacagca ucggcugggc cguggucaag       60

gaggcccaga acgacaacga gaccuccgag aucguggacc ugggggugcg cgucaacccc      120

cucacgaccg acgagaagac caacuucgag aagggcaagc cgaucagcac caacgcggac      180

cggacgcuga agcgcuccgc ccggcgcaac cugcagcggu acaagcuccg ccggaaggag      240

cugaucgagc ugcucaagga gcaccagcug aucagcaccg acaccauccu gaccgagaac      300

gggaagggca cguccuucca gacccucgag cugcgcgcca agagcgccaa ggagaagauc      360

ucccuggagg agcucgcccg gguguuccug accaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg cgaagaacga ggacgagggg cagcugaucg acggcaugga gaucgccaag      480

cgccucuacg aggagaaccu gaccacgggg cagcugagcc uccagcugcu gaaggagggc      540

aagaaguccc uccccgacua cuaccggagc gaccugcagu ucgagcugga caaggugugg      600

aacuuccaga agcaguucca cucccacauc cucaccgacg aguucuacgc ccagcugaag      660

gggaagggcc agcgcgccac cagcgcgcug uuccucgcca aguaccagau cuacaccgcc      720

gagaacaagg gcucccggga ggacaagaag cugcaggccu accaguggcg cgucgacgcc      780

cugagcaagg agcucacgcg ggagcagcug gcguucguga ucaccgagau caacaacaac      840

cugaacaacu ccagcgggua ccucggcgcc aucuccgacc gcagcaagga gcuguacuuc      900

aacaacgaga ccguggggga guaccugugg aagcagaucg ccgccaaccc ccacaccagc      960

cucaagaacc aggucuucua ccggcaggac uaccuggacg aguucgagca gaucuggaac     1020

acgcaggccc aguucuaccc ccagauccug accgacgagc ucaagaagga gcugcgcgac     1080

guggugaucu ucuaccagcg gaagcugaag ucccagaagu cccucgucuc cuucugcgag     1140

uucgagaaca aggagaucga gaucaagaag gaggacggca ccagcaccaa gaaggugauc     1200

ggccugaagg uggcgccgaa guccagcccc cuguuccagg aguucaagau cuggcagaac     1260

cucaacaacg uccugauccg caagaagggg uccaagacga agaaggccgc caagaaccag     1320

caggccgugc uguucgagga ggagaagacc aucagcgagc uggaccucga ggccaagcag     1380

gcgcuguuca acgagcugaa ccucaagggc aaccugaagg ccgacuacug ccuggagauc     1440

cucggguacu cccccaagga gugggagaug aacuacagcg ugcuggaggg caaccgcacc     1500

aacaaggccc uguacgaggc cuaccucaag aucaccgacc uggaggggua cgacgccaag     1560

aaccaccuga aggucaagcu caacaaggac gagguggagc uggacgaccu gaaggugccc     1620

gcguccgaga ucaaggacau gguccaggcc aucuucaagg aggccggcau caacacggac     1680

auccuccacu ucaacgccga gcuggacggc aaggacuucg agaaccaggc gagcuaccag     1740

cuguggcacc uccuguacuc cuacgagggg gacgacagca aguccggcaa cgagcugcuc     1800

uacaagcugc uggagaccaa guucggguuc aagaaggagu acgcccagau ccucgccaac     1860

gugagccugu ccgacgacua cggcagccug uccaccaagg ccaugcggaa gauccacccg     1920

uacaucaagg agaacaaguu cagcaccgcc ugcgagcucg cgggguaccg ccacagcaag     1980

cacucccuga cgaaggagga gaacgacaac cgggugcuga aggacagccu cgagguccug     2040

aagaagaacu cccugcgcca gcccgugguc gagaagaucc ucaaccagau gaucaaccug     2100

gugaacgccc ugcucaagaa guaccggacc gagaacccca acuucaaguu cgacgagauc     2160

cgcaucgagc uggcccggga gcugaagaag aacgccgagg agcgcgccaa caugaccgcg     2220

aacaucaaca aggccaagac cgagcacgag aagaucauca agauccucaa gaacgaguuc     2280

ggccugccca acccgacgcg gaacgacauc auccgcuacc ggcuguacga ggagcucaag     2340

gccaacggcu acaaggaccu guacaccaac aucaagaucc cccaggagaa gcuguucacc     2400

aaggagaucg acaucgagca caucaucccc aagagccgcg uguucgacga cuccuucagc     2460

aacaagaccc ucuccuuccg gaagaccaac cuggacaagg gggagcgcac ggccuucgac     2520

uacauggaga gcaaguucgg cgccgacaag cuggaggagu acgucgcgcg gguggaggac     2580

cucuacaaga acaacaucau cuccaaggcc aaguaccaga agcugcugaa gaaggagagc     2640

gagaucgggg acggcuucgu ggagcgcgac cuccgggaga cccaguacau cgccaagaag     2700

gccaaggaga uccugcugca gaucaccccc aacguccucu ccaccagcgg gggcaucacg     2760

gaccgccugc gggaggacug ggaccugauc aacgugauga aggagcucaa ccugccgaag     2820

uaccgcgcgc ugggccucac cgagauggag gaccgcaagu acgggcagca ggucgaggug     2880

aucaucgacu ggaccaagcg gaacgaccac cgccaccacg ccauggacgc ccugaccgug     2940

gccuucacga agcacaacca cauccaguac cugaacuacc ucaacgcccg gaagaacgag     3000

aagcacaagg agcacuccaa caucaugggc auccagcagc uggagaccau caaggugacc     3060

gacaagaacg ggaacgagaa gcgcgucuuc aaggcgccca ugcccaacuu ccgccaggug     3120

accaaggccu uccuggagaa cauccucauc agccacaagg ccaagaacaa gguggucacg     3180

aagaacaaga acaagguggc cggcuccaac aaggugcagg agguccugac gccccggggc     3240

cagcugcaca aggagaccgu guacgggaag uaccaguacu acgugcagaa ggaggagaag     3300

aucggcggga aguucgaccu cgagaccauc aagaaggucg ccaacccgcu guaccagagc     3360

cugcucaaga agcggcugga ggagaacggc ggggacccca agaaggcguu caccggcaag     3420

uacucccugg ccaagaaccc caucuaccuc gacgacgccc agaccgagca gcugcccgag     3480

aaggugaagc ugguguggcu cgagaacagc uucagcaucc gcaaggacau caccccggag     3540

aacuucaagg acgagaagcu gaucgacaag auccuggacg agggcgucaa gcggauccuc     3600

aaggagcgcc ugcaguccuu cgggaacgac gccaagaagg ccuucagcga ccuggacaag     3660

aaccccaucu ggcucaacaa ggagaagggc aucaccauca agcgggugac gaucuccggg     3720

guggcgaacg ccgagccccu gcacaccaag aaggaccacc ugggcaagga gauccucgac     3780

aaggagggga agcccauccc ggucgacuuc aucagcaccg gcuccaacca ccacguggcc     3840

guguaccgcg acgagaaggg caaccugcag gagaaggucg ugagcguguu cgaggccguc     3900

gugcgggcga accaggggcu gcccaucauc gacaagaccu acaaccagca ccucggcugg     3960

gaguuccugu ucacgaugaa gcagaacgag auguucgugu uccccaacga guccaccggg     4020

uucaacccga acgacaucga ccugcucgac cccaagaaca agcgcaucau cagccccaac     4080

cuguuccggg uccagaccau cuccguggug aaguacggca acgccaucgu ccgcgacuuc     4140

aaguuccggc accaccugga gaccacggug gaggacaaga aggagcugca ggggaagacc     4200

uaccagcaga ucaagagccu cagcccccug aacgacauca ucaaggugcg caucaaccac     4260

cugggcgaca ucgucagcgu gggcgaguac uga                                  4293


<210>  2187
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2187
augaagcaca uccugggccu cgaccugggg accaacagca ucggcugggc cuacgugaag       60

gaggccgaga acgacaccga gacguccgag aucgucgacc ugggggugcg cgugaacccc      120

cucaccaccg acgagaagac caacuucgag aagggcaagc cgaucagcac gaacgcggac      180

cggacccuga agcgcuccgc ccggcgcaac cugcagcggu acaagcuccg ccggaaggag      240

cugaucgagc ugcucaagga gcaccagcug aucagcgaga acaccauccu gaccgagaac      300

gggaagggca cgaccuucca gacccucgag cugcgcgcca aguccgccaa ggagaagauc      360

agccugggcg agcucgcccg ggucuuccug accaucaaca agaagcgcgg guacaagucc      420

agccggaagg cgaagaacga ggacgagggc cagcugaucg acgggaugga gaucgccaag      480

cgccucuacg aggagaaccu gacgaccggc cagcuguccc uccagcugcu gaaggagggg      540

aagaaguccc uccccgacua cuaccggucc gaccugcagu ucgagcugga caaggugugg      600

aacuuccaga agcaguucca cagcgacauc cucaccgacg aguucuacgc ccagcugaag      660

ggcaagggcc agcgcgccac cuccgcgcug uuccucgcca aguaccagau cuacacggcc      720

gagaacaagg ggagccggga ggacaagaag cugcaggccu accaguggcg cguggacgcc      780

cuguccaagg agcucacccg ggagcagcug gcguucguca ucaccgagau caacaacaac      840

cugaacaaca gcuccggcua ccucggggcc aucagcgacc gcuccaagga gcuguacuuc      900

aacaacgaga ccgugggcga guaccugugg aagcagaucg ccgccaaccc ccacacgagc      960

cucaagaacc agguguucua ccggcaggac uaccuggacg aguucgagca gaucuggaac     1020

acccaggccc aguucuaccc ccagauccug accgacgagc ucaagaagga gcugcgcgac     1080

gucgugaucu ucuaccagcg gaagcugaag ucccagaagu cccucgugag cuucugcgag     1140

uucgagaaca aggagaucga gaucaagaag gaggacggca ccuccacgaa gaaggucauc     1200

gggcugaagg uggcgcccaa gagcagcccc cuguuccagg aguucaagau cuggcagaac     1260

cucaacaacg ugcugauccg caagaagggc agcaagacca agaaggccgc caagaaccag     1320

caggccgucc uguucgagga ggagaagacc aucuccgagc uggaccucga ggccaagcag     1380

gcgcuguuca acgagcugaa ccucaagggg aaccugaagg ccgacuacug ccuggagauc     1440

cucggcuaca gccccaagga gugggagaug aacuacuccg ugcuggaggg gaaccgcacc     1500

aacaaggccc uguacgaggc cuaccucaag aucacggacc uggagggcua cgacgccaag     1560

aaccaccuga aggugaagcu caacaaggac gaggucgagc uggacgaccu gaaggugccc     1620

gcgagcgaga ucaaggagau ggugcaggcc aucuucaagg aggccggcau caacaccgac     1680

auccuccacu ucaacgccga gcuggacggg aaggacuucg agaaccaggc guccuaccag     1740

cuguggcacc uccuguacag cuacgagggc gacgacucca agagcgggaa cgagcugcuc     1800

uacaagcugc uggagaccaa guucggcuuc aagaaggagu acgcccagau ccucgccaac     1860

gucucccuga gcgacgacua cgggaaccug uccaccaagg ccaugcggaa gaucuacccg     1920

uacaucaagg agaacaaguu cagcacggcc ugcgagcucg cgggcuaccg ccacuccaag     1980

cacagccuga ccaaggagga gaacgacaac cgggugcuga aggacagccu cgaggugcug     2040

aagaagaacu cccugcgcca gcccgucgug gagaagaucc ucaaccagau gaucaaccug     2100

gucaacgccc ugcucaagaa guaccggacc gagaacccca acuucaaguu cgacgagauc     2160

cgcaucgagc uggcccggga gcugaagaag aacgccgagg agcgcgccaa caugaccgcg     2220

aacaucaaca aggccaagac ggagcacgag aagaucauca agauccucaa gaacgaguuc     2280

ggccugccca acccgacccg gaacgacauc auccgcuacc ggcuguacga ggagcucaag     2340

gccaacgggu acaaggaccu guacaccaac aucaagaucc cccaggagaa gcuguucacc     2400

aaggagaucg acaucgagca caucaucccc aagagccgcg uguucgacga cuccuucagc     2460

aacaagaccc ucuccuuccg gaagacgaac cuggacaagg gcgagcgcac cgccuucgac     2520

uacauggaga gcaaguucgg ggccgacaag cuggaggagu acguggcgcg ggucgaggac     2580

cucuacaaga acaacaucau cuccaaggcc aaguaccaga agcugcugaa gaaggagagc     2640

gagaucggcg acggguucgu ggagcgcgac cuccgggaga cccaguacau cgccaagaag     2700

gccaaggaga uccugcugca gaucacccgc aacgugcucu ccacgagcgg cggcaucacc     2760

gaccggcugc gcgaggacug ggaccugguc aacgugauga aggagcucaa ccugcccaag     2820

uaccgcgcgc uggggcucac cgagauggag gaccggaagu acggccagca ggucgaggug     2880

aucaucgacu ggaccaagcg caacgaccac cggcaccacg ccauggacgc ccugacggug     2940

gccuucacca agcacaacca cauccaguac cugaacuacc ucaacgcccg caagaacgag     3000

aagcaccggg agcacuccaa caucaugggg auccagcagc uggagaccau caaggugacc     3060

gacaagaacg gcaacgagaa gcgcgucuuc aaggcgccga ugcccaacuu ccgccaggug     3120

acgaaggccu uccuggagaa cgugcucauc agccacaagg ccaagaacaa ggucgugacc     3180

aagaacaaga acaagguggc cggcuccaac aagguccagg aggugcugac cccgcggggg     3240

cagcugcaca aggagaccgu guacggcaag uaccaguacu acguccagaa ggaggagaag     3300

aucgggggca aguucgaccu cgagacgauc aagaaggugg ccaacccccu guaccagagc     3360

cugcucaaga agcggcugga ggagaacggg ggcgacccga agaaggcguu caccggcaag     3420

aacucccugg ccaagaaccc caucuaccuc gacgacgccc agaccgagca gcugcccgag     3480

aaggugaagc uggucuggcu cgagaacagc uucuccaucc gcaaggacau cacccccgag     3540

aacuucaagg acgagaagcu gaucgacaag auccuggacg agggggugaa gcggauccuc     3600

aaggagcgcc ugcagagcuu cggcaacgac gccaagaagg ccuucagcga ccuggacaag     3660

aacccgaucu ggcucaacaa ggagaagggg aucacgauca agcgggugac caucuccggc     3720

gucgcgaacg ccgagccccu gcacaccaag aaggaccacc uggggaagga gauccucgac     3780

aaggagggca agcccauccc cguggacuuc aucagcaccg gcuccaacca ccacguggcc     3840

gucuaccgcg acgagaaggg gaaccugcag gagaaggugg ugagcgucuu cgaggccgug     3900

gugcgggcga accagggccu gccgaucauc gacaagacgu acaacgagca ccucgggugg     3960

gaguuccugu ucaccaugaa gcagaacgag auguucgucu uccccaacga guccaccggc     4020

uucaacccca acgacaucga ccugcucgac ccgaagaaca agcgcaucau cagccccaac     4080

cuguuccggg ugcagaccau cuccgugguc aaguacggga acgccaucgu gcgcgacuuc     4140

aaguuccggc accaccugga gacgaccgug gaggacaaga aggagcugca gggcaagacc     4200

uaccagcaga ucaagagccu cagcccccug aacgacauca ucaagguccg caucaaccac     4260

cugggcgaca ucgugagcgu gggggaguac uga                                  4293


<210>  2188
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2188
auggcccaga aguacauccu gggccucgac cuggggacca acagcaucgg cugggccgug       60

gucaaggagg cggagaacga caacgagcag uccgagaucg uggacguggg gguccgcgug      120

aacccccuga ccacggacga gaagaccaac uucgagaagg gcaagccgau cagcaccaac      180

gccgaccgga cccucaagcg cggggcccgg cgcaaccugc agcgguacaa gcugcgccgg      240

aaggagcuca ucaagauccu gaaggagcac cagcugaucu ccgacgacac gauccucgcc      300

gagaacggca agggcaccac cuaccagacc cugaagcugc gcgccaagag cuccaaggag      360

aagaucagcc ucgaggagcu ggcgcgggug uuccugacga ucaacaagaa gcgcggguac      420

aaguccagcc ggaagaccaa gaacgaggac gagggccagc ucaucgacgg gauggagauc      480

gccaagcgcc uguacgagga gaaccugacc accggccagc ucucccugca gcugcucaag      540

gaggggaaga agucccugcc cgacuacuac cggagcgacc uggagcucga gcuggacaag      600

gucuggaacu uccagaagca guuccacagc gacauccuga cggacgaguu cuacacccag      660

cucaagggca agggccagcg cgccaccucc gcccuguucc uggagaagua ccagaucuac      720

accguggaga acaaggggaa ccgggaggag aagaaccucc aggcguacca guggcgcgug      780

gacgcccuga gcaaggagcu gacgcgggag gagcucgccu ucgucaucac cgagaucaac      840

aacaaccuga acaacuccag cggcuaccug ggggccaucu ccgaccgcag caaggagcuc      900

uacuucaaca acgagaccgu gggcgaguac cuguggaagc agaucgccgc gaacccccac      960

accucccuga agaaccaggu guucuaccgg caggacuacc ucgacgaguu cgagcgcauc     1020

uggaacacgc aggcccaguu cuacccccag auccugaccg agagccugaa gaaggagcuc     1080

cgggacgucg ugaucuucua ccagcgcaag cucaagagcc agaagucccu ggugagcuuc     1140

ugcgaguucg agaacaagga gaucgagauc aagaacgagg acggcaccac cacgaagaag     1200

gucaucgggc ucaagguggc cccgaagucc agcccccugu uccaggaguu caagaucugg     1260

cagaaccuga acaacgugcu cauccgcaag aagggcucca agaccgagaa ggucgccaag     1320

aaccagcagg ucgugcuguu cgaggaggag aagaccauca gcgagcugga ccuggaggcc     1380

aagcaggcgc uguucaacga gcugaaccug aaggggaacc ucaaggccga cuacugccug     1440

gagauccugg gcuacuccag caaggagugg gagaugaacu acuccgugcu cgaggggaac     1500

cggaccaaca aggcccugua cgaggccuac cugaagauca cggaccucga gggcuacgac     1560

gccaagaacu accugaaggu caagagcaac aaggacgagg uggagcugga cgaccucaag     1620

gugcccgcgu ccgagaucaa ggagauggug caggccaucu ucaaggaggc cggcaucaac     1680

accgacaucc ugcacuucga cgccgagcug gacgggaagg acuucgagaa ccagggcagc     1740

uacaagcucu ggcaccuccu guacuccuac gagggggacg acagcaaguc cggcaacgag     1800

cugcuguaca agcugcucga gaccaaguuc gggcugaaga aggaguacgc ccagauccug     1860

gcgaacauca gccucuccga cgacuacggc aaccugagca ccaaggccau gcgcaagauc     1920

caccccuaca ucaaggagaa caaguucucc acggccugcg agcuggccgg cuaccggcac     1980

agcaagcaca gccucaccaa ggaggagaac gagaagcgcg uccugaagga cgggcuggag     2040

gugcucaaga agaacucccu gcggcagccg guggucgaga agauccugaa ccagaugauc     2100

aaccucguga acgcgcugcu gaagaaguac cgcaccgaga accccaacuu caaguucgac     2160

gagauccgga ucgagcucgc ccgcgagcug aagaagaacg ccgaggagcg ggccaacaug     2220

accgccaaca ucaacaaggc gaagacggag cacgagaaga ucgacaagau ccucaagagc     2280

gaguucggcc ugcccaaccc cacccgcaac gacaucaucc gguaccgccu guacgaggag     2340

cugaaggcca acggguacaa ggaccucuac accaaccggu acaucuccag cgagaagcug     2400

uucuccaagg agaucgacau cgagcacauc auccccaaga gccgcguguu cgacgacucc     2460

uucagcaaca agaccaucgu cuaccggacc gacaaccugg acaagggcga gcagacggcc     2520

uacgacuaca ucgaguccaa guucggggcc gagaagcuca aggaguacau gacccgcgug     2580

aaggaccugu accagaagaa gaucaucagc aaggccaagu accagaagcu gcucaagaag     2640

gaguccgaga ucggcgacgg cuucguggag cgggaccugc gcgagaccca guacaucgcg     2700

aagaaggcca aggagauccu gcuccagauc acccacaacg uccugagcac guccgggggc     2760

aucaccgacc ggcugcgcaa ggacuggggg cucgugaacg ugaugaagga gcugaaccug     2820

ccgaaguacc gcgcccucgg ccugaccaag auggaggacc ggaaguacgg ccagcagguc     2880

gaggugauca ccgacuggac gaagcgcaac gaccugcggc accacgccau ggacgcccuc     2940

accguggcgu ucaccaagca caaccacauc caguaccuga acuaccugaa cgcccgcaag     3000

aacgagaagc acaaggagca caacaacauc auggccaucc agcagcucga gaccaucaag     3060

gucacggacg agaacgggaa cgagaagcgg aucuucaagg cccccaugcc caacuuccgc     3120

cagguggcga agaccuuccu ggagaacgug cugaucagcc acaaggccaa gaacaagguc     3180

gugaccaaga acaagaacaa gaucuccggc agcaacaagg ugcaggaggu ccucaccccg     3240

cgggggcagc ugcacaagga gacgguguac ggcaaguacc aguacuacgu gcagaaggag     3300

gagaagaucg gggccaaguu cgacuccgag accaucgaga aggucgccaa cccccuguac     3360

cagagccucc ugaagaagcg ccuggaggag aacggcggcg accccaagaa ggccuucacc     3420

gggaagaaca gccucgcgaa gaaccccauc uaccuggacg acacccagac ggagcagcug     3480

ccggagaagg ugaagcucgu guggcuggag aacuccuuca gcauccggaa ggacaucacc     3540

cccgagaacu ucaaggacga gaagcugauc gacaagaucc ucgacgaggg cgucaagcgc     3600

auccugaagg agcggcugca guccuucggg aacgacgcca agaaggccuu cagcgaccuc     3660

gagaagaacc ccaucuggcu gaacaaggag aagggcaucg ccaucaagcg cgugaccauc     3720

uccggggugg ccaacgcgga gccccugcac accaagaagg accaccucgg caaggagauc     3780

cuggacaagg agggcaagcc gauccccguc gacuucauca gcacggggaa caaccaccac     3840

guggccgugu accgggacga gaagggcaac cugcaggaga agguccucuc cguguucgag     3900

gccguggucc gcgccaacca ggggcugccc aucaucgaga agaccuacaa ccagcaccug     3960

ggcugggagu uccucuucag caugaagcag aacgagaugu ucguguuccc caacgagucc     4020

accggguuca acccgaacga caucgaccug cuggacccca agaacaagcg gaucaucagc     4080

cccaaccucu ucaaggugca gaagcuggcc accaagaacu acauguucac gcaccaccug     4140

gagaccaagu ccaucgacgg cgacaugcug aagaacaaga aggagcucag cggcauccug     4200

uacaacuuca uccagacccc gcagaagcug uacgggauca ucaagguccg caucaaccac     4260

cucggcgaca ucguguccgu gggggaguac uga                                  4293


<210>  2189
<211>  4296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2189
augaagcgca uccugggccu cgaccugggg agcuccagca ucggcugggc ccuggugaac       60

gaggccgagu ccaagaacga gcggagcucc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagagcau caacuucgag aagggcaagu ccaucaccac gaacgcggac      180

cggacccuga agcgcagcau gcggcgcaac cuccaccggu acaagcugcg ccgggaggcc      240

cugaucgagg ugcucaagga gugcaaguuc aucaccgagg acaccauccu guccgagaac      300

gggaaccgca cgaccuucga gaccuaccgg cugcgcguga aggcccucac cgaggagauc      360

agccuggagg aguucgcccg gguccugauc augaucaaca agaagcgcgg cuacaagucc      420

agccggaagg ccggcggggc ggaggaggac acgcucaucg acggcaugga caucgcccgc      480

aagcuguacg acgccaaccu gaccccgggg gagcucugcc ugcagcugcu cuccgccggc      540

aagaaggugc ugcccgacuu cuaccggagc gaccugcaga acgagcucga ccgcaucugg      600

gagaagcaga aggaguacua ccccgggauc cugaccgcgg cccugaagga ggagcuccgg      660

ggcaagaagc gcgacgccgu gugggccauc ugcgccaacg cguucgucug gaaggagacc      720

uacacggagu ggaacaacga ggaguccaag aacgagcaga ucgagaagga gcacaagcug      780

gagggcaucu acagcaagcg gaagcgcgac gaggccaaga aggagaaccu gcaguggcgc      840

guguccgggc ucaaggagaa gcugagccug gagcagcucg ugaucauccu gcaggagauc      900

aacacccaga ucaacaacuc cagcggcuac cuggggacca ucagcgaccg guccaaggag      960

cucuacuuca acaagcagac cgucggccag uaccugaugg acauccugga caacaacccc     1020

aacgccagcc uccgcaacau cguguucuac cggcaggacu accuggacga guucaacgug     1080

cugugggaga agcaggccga guuccacaag gagcucacgg gcgagcugaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cugaaguccc agaaggggcu caucagcuuc     1200

ugcgaguucg agucccggca gaucgagguc aagaucgacg gcaagcgcaa gauccggacc     1260

guggggaacc gcgugauccc gcggagcagc ccccuguucc aggaguucaa gaucuggcag     1320

auccugaaca acaucgaggu caccgccgug ggcaacaaga acaagcagcg caugcagaac     1380

ggguccaacg agcccgacga caccgagcag cucgagcuga acggccggcg cuaccuguac     1440

caggaggaga aggagcugcu cgcgaaggag cuguucgugc gggacaagau gacgaagcug     1500

gacguccucc gccugcuguu cgacaacccc gaggagcucg accugaacuu caagaccauc     1560

gacggcaaca agaccgggua ccuguucuuc caggccuacu ccaagaugau cgagaugagc     1620

ggccacgagc cgguggacuu caagaagccc aucgagaaga ucguggagca ggucaagucc     1680

guguucgucc uccugaacug gaacaccgac auccugagcu ucgacuccaa caaggagcuc     1740

gacaagcaga gcuacuacaa gcuguggcac cuccucuacu ccuucgaggg ggacaacacg     1800

cccaccggca acgggugccu ggugcagaag aucauggaca gcuacggccu gggcaaggag     1860

uucgccaaga uccucgccaa caucaccuuc caggaggacu acgggucccu gagcgccaag     1920

gcgauccaca agauccugcc ccaccucaag gagggcaacc aguacgacgu ggccugcgag     1980

uacgccgggu accggcacuc caagagcagc cugaccaagg aggagaucgc caacaagguc     2040

cugaaggaca agcucgaggu gcugccgcgc aacucccugc ggaaccccgu ggucgagaag     2100

auccucaacc agauggugaa cgugaucaac gccaucaucg aggcguacgg ccgccccgac     2160

gagauccgcg ucgagcuggc ccgggagcug aagaagaacg ccaaggagcg cgagaagcuc     2220

accaagagca ucgccgagac caccaagacc cacgaggcgc acaagaagcu gcugcaggag     2280

gaguucgggc ucaagaacgu gggccggucc gacauccugc gcuacaagcu guacaaggag     2340

cucgagagcc ggggcuacaa gacgcuguac uccaacaccu acaucccccg cgagaagcug     2400

uucagcaagg aguucgacau cgagcacauc aucccgcagg cccggcuguu cgacgacucc     2460

uucagcaaca agacccugga ggcccgcaac gugaacaucg agaaggggaa ccggaccgcc     2520

uacgacuucg ucaaggagaa guucggcgag uccgggacgg acaacagccu ggacugcuac     2580

cucaacaaca ucgaggugcu guucgugucc ggcaagauca gcaagaccaa guacaccaag     2640

cugaagauga ccgagcagga cauccccggc ggguucaucg agcgcgaccu ccggaacacc     2700

caguacaucg ccaagaaggc gcuguccaug cugaacgaga ucagccgcug cgucguggcc     2760

acguccggca gcaucaccga caagcuccgg gaggacuggc agcuggugga cgucaugaag     2820

gagcugaacu gggagaagua caaggcccuc gggcuggugg aguacuucga ggacaaggac     2880

ggcaagcaga ucgggcgcau caaggacugg uccaagcgga acgaccaccg ccaccacgcc     2940

auggacgccc ugaccguggc guucaccaag gaggucuuca uccaguacuu caacaacaag     3000

aacgccagcc ucaaccccaa cacgaacgag uacgccauca agaacaagua cuuccagaac     3060

ggccgggcca ucgcccccau cccgcugaac gaguuccgcg cggaggccaa gaagcaccug     3120

gagaacaccc ucaucagcau caaggccaag aacaagguga ucaccaacaa caucaacaag     3180

accaagaagc ggggcggggu gaacacgaag aagcagcaga cgccccgcgg ccagcugcac     3240

cuggagacca ucuacgggug cugccggcag uaccucacca aggaggagaa ggucaacgcc     3300

uccuucgacg ugagcaagau cgcgacggug uccagcuccg ucuaccgcga cgcccugcug     3360

aagcgccucc aggagcacca caacgacccc aagaaggccu ucgccggccg gaacagccug     3420

gacaagaagc ccguguggcu ggacaaggag cagauccgca aggugccgga gaaggucaag     3480

accgugaccu ucgagaccau cuacacgauc cggaaggaga ucucccccga ccuccgcgug     3540

gacaagauca ucgacagcgg gguccggaag augcugaccg gccgccugaa ggaguacggc     3600

aacgacgcca agaaggcguu cuccaaccuc gacgagaagc ccaucuggcu gaacaaggag     3660

aaggggaucc ccaucaagcg ggugaccauc agcggcaucu ccaacgccca gagccugcac     3720

gugaagaagg acaaggacgg gaagccgguc cucgacggca acgggcgcaa cauccccgug     3780

gacuucguga acaccggcaa caaccaccac gucgccgugu accggcgccc cgugaucgac     3840

aagaacggcc agauggucau cgacgagaac gggaaccuga aguacgagcu ggaggaggug     3900

guggucacgu ucuucgacgc cgugacccgg gugaaccucg gccagccgau caucgacaag     3960

gacuacaagg ccuccgaggg guggcaguuc cuguucagca ugaagcagaa cgaguacuuc     4020

gucuucccca acaagaagac cggcuucaac cccaaggaga ucgaccugcu cgacgcggac     4080

aacuacgggc ugaucucccc caaccuguuc cgcgugcaga aguucaccca caagaacuac     4140

guguuccggc accaccucga gacgaccauc aaggacacca gcuccauccu gcgcggcauc     4200

accuggaucg acuuccggag cuccaagggc cuggacagca ucgucaaggu gcgcgucaac     4260

cacaucgggc agaucgugag cgugggcgag uacuga                               4296


<210>  2190
<211>  4299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2190
augaagaaga uccugggccu cgaccugggg agcaacucca ucggcugggc ccuggugaac       60

gaggccgaga accgcgacga ggcgagcucc aucaucaagc ucgggguccg ggugaacccc      120

cugaccgugg acgagaugca gaacuucgag aagggcaaga gcaucaccac gaacgccgac      180

cgcacccuga agcgguccau gcgccggaac cuccagcgcu acaagcugcg gcgcgacgcc      240

cugaucaagc uccugaagaa ggucggguuc aucgccgacg gcaccauccu gagcgagcag      300

ggcaaccggu ccaccuucga gacguaccgc cuccgggcca aggcgccgga cgaggagauc      360

agccugcagg agcuggcccg cgugcuccug augaucaaca agaagcgggg guacaagucc      420

agccgcaaga ucaaggagaa ggaggccggc acccugaucg acgggaugaa gguggccaag      480

aagcucuacg acgaggaccu gacccccggc cagcugugcc uccagcugcu ggaguccggg      540

aagaaguccc uccccgacuu cuacagcagc gaccugcagg aggaguucga ccggaucugg      600

aacuuccaga agcaguucaa ccccaaggcg uucugcgaca ccgccaagga ggagaucaag      660

ggcaagaaca agucccagac gugggccauc cuggccaagu acuucaucug gaccgacgag      720

auggucgugu ggaacgagaa ggagagcaag accgagcgcg uggagaagga gugcaagcuc      780

gucggccuga agcggaccgu gaagaaggag gagcagcgca aggagaacua ccgguggcgc      840

gugcaggccc ugacggagca gcucgaccug gggaaggucg cgguggugcu gcaggagauc      900

aacggccaga ucuccaccag cuccggguac cucggcgcca ucagcgaccg gagcaagguc      960

cuguacuuca accgccagac cgugggccag uaccagaugg ccgagcugga gaagaacccg     1020

aacaccuccc uccgcaacau gguguucuac cggcaggacu accuggacga guucgacaag     1080

aucugggaga agcaggccga guuccacaag gagcugacgg ucgagcucaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cucaagagcc agaaggggcu gaucggcuuc     1200

ugcgaguucg agaaccggga gaucauggug gagaagaacg ggaagaugca ggccaaggug     1260

gucggcugca aggugauccc ccgcucccac ccccucuucc aggaguucaa gaucuggcag     1320

acccugaaca acguggaggu cuucgcgggg gagaagaagu gcaagcggaa gcgcaccgac     1380

gccuuccccg gccuguucga cggcaccgag gacacgcuca ugguggaggg guggcgguac     1440

cuguaccagg aggagaagga gauccuggcc aaggagcuca gcgucaagaa gucccugaag     1500

aaggaguaca uccugaagcu gcuguucgac aacccgcagg accuggacau gaacuucgag     1560

cagaucgagg gcaacgacac cgggaacgug cuguucagcg ccuacuccaa gaugaucgag     1620

aaguacggcu acgagccccu cgacuucggc aagagcaccg acgagaucau cgagcgccuc     1680

caggugaucu ucaccgaccu gggguggaac acggaccugc uguucgucga ccugaacaag     1740

gagggcaagg accucgagaa gcagcccuac uucaagcugu ggcaccuccu guacuccuuc     1800

gagggggaca acacccccac cggcaacggg aagcuggugg agaagcugau ggagcucugc     1860

cacgugggca aggagugcgc ccuggagcug gucaacauca gcuuccagaa cgacuacggg     1920

ucccucagcg cgaaggccau caagaagauc cugccguacc ugaaggaggg caaccgguac     1980

gacguggccu gcggcuacgc cggguaccgc cacuccaaga gcucccucac caaggaggag     2040

aucgagaaca aggugcugaa ggacaagcug gacauccucc ccaagaacag ccugcggaac     2100

cccgucaucg agaagauccu gaaccagaug gugaacguga ucaacgccau caucgacacg     2160

uacggcaagc ccgacgagau ccgcgucgag cucgcgcggg agcugaagaa guccgccaag     2220

gagcgcgacg agcugaccaa ggccaucgcc aagagcaccc gggagcacga ggagauccgc     2280

aaggugcucc aggaggaguu cgggaugauc aacguguccc ggaacgacau cauccgcuac     2340

aagcuguacg aggagcugaa ggacaacggc uacaagaccc ucuacagcaa ccaguacauc     2400

ccgaaggaga aggucuucag caaggagauc gacaucgagc acaucauccc ccaggcgcgc     2460

cuguucgacg acucccugag caacaagacg cucgaguaca aggccaucaa caucgagaag     2520

ggcaacaaga ccgccuacga cuucgugaag gagaaguacg gggagaaggg ccugcagcag     2580

uaccugaacc ggugcgaguc ccucuucaag gggcaccgca ccaagcugcg gaagcugaag     2640

augggccagg agaacauccc cgagggguuc aucgaccgcg accuccggaa cacccaguac     2700

aucgccaaga aggcccuggc gaugcugaac gagaucugcc gccggguggu cgccacgacc     2760

ggcagcauca ccgaccgccu ccgggaggac uggcagcugg uggacgugcu gaaggagcuc     2820

aaccugccca aguacgagac ccugggccuc gucgaggugu acgaggacaa ggacgggcgc     2880

aagaucaagc ggaucaagga cuggaccaag cgcaacgacc accggcacca cgccauggac     2940

gcccugacgg uggccuucac caaggacguc uucauccagu acuucaacaa caagaacgcg     3000

gccuggcagg ccgacugcaa ggagcacucc gacaucaucg gcaucaagac ccgcuacuuc     3060

gagaacgggc gggcccugcc gcccaugccc cucgugcgcu uccgggccga ggccaagagc     3120

caccuggaga cccugcucgu guccaucaag gcgaagaaca aggucgugac gaacaacgug     3180

aacugcacca agcgcaaggg cggggagaac aagaagcugc agcagacgcc ccggggccag     3240

cugcaccucg agaccaucua cggcagccac cgccaguacg ucaugaagga ggagaaggug     3300

aacgccuccu ucgacagcuc caagaucgcc accgugugca agcaggcgua ccggaacgcc     3360

cugcuggagc gccucgccgc cuucgggaac gacccgaaga aggccuucac cggcaagaac     3420

agccuggaga agaaccccgu cuaccuggac cgcgagcaga ccgcgcgggu gcccgagcgc     3480

gugcggaccg ucgagcucga gacgguguac accauccgca agcccgugga cccgaaccug     3540

uccgucgaca agguggugga cgccaagguc cggagcaucc uggagcagcg ccuccgggag     3600

uacgacgggg aggccaagcg cgccuucgug aaccuggacg agaaccccau cuggcugaac     3660

gaggagaagg gcaucgccau caagcgggug accauccgcg ggaucaacaa gguccagucc     3720

cuccacggca agaaggacaa gaacggcaac cugauccugg acgagaccgg gaagaagauc     3780

cccguggacu ucgugaacac gggcaacaac caccacgucg cgaucuaccg gaagccggug     3840

cucgacaaga ccgggcagga gaugcuggac aacgagggca accccgugua cgagcuggac     3900

gaggucgugg ucagcuucuu cgaggccgug acccgcguga accucgggcu gcccgucauc     3960

gacaagaacu acaacaaguc cgagggcugg caguucaugu ucaccaugaa gcagaacgag     4020

uacuucgugu uccccaacga gaagacgggc uucgacccga aggaggugga ccugcucaac     4080

cccgagaacu acgccgucau cagccccaac cuguuccggg ugcagaagau caccgagaag     4140

gacuacaugu uccgccacca ccuggagacc aagguggucg acgacaaguu ccucaaggag     4200

aucaccugga agcggauccu gacgcccaac aagcuggagg ggaucgugaa ggugcgcguc     4260

aaccacaucg gccagaucgu gagcgugggg gaguacuga                            4299


<210>  2191
<211>  4299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2191
augaagaaga uccugggccu cgaccugggg agcaacucca ucggcugggc ccuggugaac       60

gaggccgaga acagcgacga ggucuccagc aucaucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagaugca gaacuucgag aagggcaagu ccaucaccac gaacgcggac      180

cggacccuga agcgcagcau gcggcgcaac cuccagcggu acaagcugcg ccgggacgcc      240

cugaucaagc uccugaagaa ggugggguuc aucaccgacg gcaccauccu guccgagcag      300

ggcaaccgca cgaccuucga gaccuaccgg cuccgcgcca aggccccgga cgaggagauc      360

agccugcagg agcuggcccg ggugcuccug augaucaaca agaagcgcgg guacaagucc      420

agccggaaga ucaaggagaa ggaggcgggc acccugaucg acgggaugga ggucgccaag      480

aagcucuacg acgaggaccu gacgcccggc cagcugugcc uccagcugcu ggaguccggg      540

aagaaguccc uccccgacuu cuacagcagc gaccugcagg aggaguucga ccgcaucugg      600

aacuuccaga agcaguucaa ccccaaggcc uucugcgaca ccgccaagga ggagaucaag      660

ggcaagaaca agucccagac cugggcgauc cuggccaagu acuucaucug gaccgacgag      720

augguggugu ggaacgagaa ggagagcaag acggagcggg ucgagaagga gugcaagcuc      780

gugggccuga agcgcaccgu gaagaaggag gagcagcgga aggagaacua cugcuggcgc      840

guccaggccc ugaccgagca gcucgaccug gagaaggugg ccgugguccu gcaggagauc      900

aacgggcaga ucuccaccag cuccggcuac cucggggcca ucagcgaccg gagcaaggug      960

cuguacuuca accgccagac ggugggccag uaccagaugg cggagcugga gaagaacccg     1020

aacaccuccc uccggaacau ggucuucuac cgccaggacu accuggacga guucgacaag     1080

aucugggaga agcaggccga guuccacaag gagcugaccg uggagcucaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cucaagagcc agaagggccu gaucggguuc     1200

ugcgaguucg agaaccggga gaucauggug gagaagaacg gcaagaugca ggccaagguc     1260

guggggugca aggugauccc ccgcucccac ccccucuucc aggaguucaa gaucuggcag     1320

acccugaaca acgucgaggu guucgccggc gagaagaagu gcaagcggaa gcgcacggac     1380

gccuuccccg ggcuguucga cggcaccgag gacacccuca uggucgaggg cuggcgguac     1440

cuguaccagg aggagaagga gauccuggcg aaggagcuca gcgugaagaa gucccugaag     1500

aaggaguaca uccugaagcu gcuguucgac aacccgcagg accuggacau gaacuucgag     1560

cagaucgagg ggaacgacac cggcaacgug cuguucagcg ccuacuccaa gaugaucgag     1620

aaguacgggu acgagccccu cgacuucggc aagcccacgg acaagaucau cgagcagcug     1680

caggucaucu ucgccgaccu gggguggaac accgaccucc uguucgugga ccugaacaag     1740

gagggcaagg accucgagaa gcagcccuac uucaagcugu ggcaccuccu guacagcuuc     1800

gagggcgaca acaccccgac cgggaacggc aagcuggugg agaagcugau ggagcucugc     1860

cacgucggga aggagugcgc ccuggagcug gugaacaucu ccuuccagaa cgacuacggc     1920

agccucuccg ccaaggcgau caagaagauc cugcccuacc ugaaggaggg gaaccgcuac     1980

gacguggccu gcggcuacgc cggcuaccgg cacagcaagu ccagccucac gaaggaggag     2040

aucgagaaca agguccugaa ggacaagcug gacauccucc ccaagaacuc ccugcgcaac     2100

cccguggugg agaagauccu gaaccagaug gucaacguga ucaacgccau caucgacacc     2160

uacgggaagc cggacgagau ccggguggag cucgcccgcg agcugaagaa guccgcgaag     2220

gagcgggagg agcugaccaa ggccaucgcc aagagcaccc gcgagcacga ggagauccgg     2280

aagguccucc aggaggaguu cggcaugauc aacgugagcc gcaacgacau cauccgguac     2340

aagcuguacg aggagcugaa ggacaacggg uacaagacgc ucuacuccaa ccaguacauc     2400

cccaaggaga agguguucag caaggagauc gacaucgagc acaucauccc ccaggcccgc     2460

cuguucgacg acagccuguc caacaagacc cucgaguaca aggcgaucaa caucgagaag     2520

ggcaacaaga ccgccuacga cuucgucaag gagaaguacg gcgagaaggg gcugcagcag     2580

uaccugaacc ggugcgagag ccucuucaag ggccagcgca ccaagcugcg caagcugaag     2640

auggggcagg agaacauccc cgagggcuuc aucgaccggg accuccgcaa cacgcaguac     2700

aucgccaaga aggcccuggc caugcugaac gagaucugcc ggcgcguggu ggcgaccacc     2760

ggguccauca ccgaccggcu ccgcgaggac uggcagcugg ucgacgugcu gaaggagcuc     2820

aaccugccga aguacgagac ccugggccuc guggaggucu acgaggacaa ggacggccgg     2880

aagaucaagc gcaucaagga cuggacgaag cggaacgacc accgccacca cgccauggac     2940

gcccugaccg uggccuucac caaggacgug uucauccagu acuucaacaa caagaacgcc     3000

gcguggcagg ccgacugcaa ggagcacagc gacaucaucg ggaucaagac ccgguacuuc     3060

gagaacggcc gcucccugcc gcccaugccc cucguccggu uccgcgccga ggccaagagc     3120

caccuggaga cgcugcucgu guccaucaag gccaagaaca agguggucac caacaacgug     3180

aacugcacca agcggaaggg cggggagaac aagaagcugc agcagagccc ccgcgggcag     3240

cugcaccucg agaccaucua cggcucccac cggcaguacg ugacgaagga ggagaagguc     3300

aacgcgagcu ucgacuccag caagaucgcc accgugugca agcaggccua ccgcaacgcc     3360

cugcuggagc ggcucgcggc cuucggcaac gaccccaaga aggccuucac cgggaagaac     3420

ucccuggaga agaaccccgu guaccugaac cgcgagcaga ccgcccgggu ccccgagcgc     3480

gugcgcacgg uggagcucga gaccgucuac accauccgga agccggugga ccccaaccug     3540

agcguggaca aggucgugga cgccaaggug cgcuccaucc uggagcagcg gcuccgcgag     3600

uacgacggcg aggcgaagcg ggccuucguc aaccuggacg agaaccccau cuggcugaac     3660

gaggagaagg ggaucgccau caagcgcgug accauccggg gcaucaacaa ggugcagagc     3720

cuccacggga agaaggacaa gaacggcaac cugauccugg acgagacggg caagaagauc     3780

ccggucgacu ucgugaacac cgggaacaac caccacgugg ccaucuaccg caagcccguc     3840

cucaacaaga ccggccagga gaugcuggac aacgagggga accccgugua cgagcuggac     3900

gaggucgugg uguccuucuu cgaggccguc acccggguga accucggccu gcccgugauc     3960

gacaagaacu acaacaagag cgaggggugg caguucaugu ucacgaugaa gcagaacgag     4020

uacuucgucu ucccgaacga gaagacccgc uucgacccca aggaggugga ccugcucaac     4080

cccgagaacu acgcggugau cagccccaac cuguuccggg uccagaagau caccgagaag     4140

gacuacaugu uccgccacca ccuggagacc aagguggugg acgacaaguu ccucaaggag     4200

aucacgugga agcggauccu gaccccgaac aagcuggagg gcaucgucaa ggugcgcgug     4260

aaccacaucg gccagaucgu cuccgugggg gaguacuga                            4299


<210>  2192
<211>  4299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2192
augaagaaca uccugggccu cgaccugggg accaccagca ucggcuucgc ccacaucgug       60

gaggacgaga acaaggagaa guccgagauc aaggagcugg ggguccgcau cgugagccuc      120

acgaccgacg agcaguccga cuucgagaag ggcaagagca ucaccaccaa cgccaaccgg      180

acgcugaagc acggggcgcg ccugaaccuc gaccgguacc agcagcgccg gaaguaccug      240

aucgaccugc uccacaaggc caaccugauc acccccucca gcauccuggc cgagaacggc      300

aagaacacca cccacuccac guggcagcuc cgcgccaagg ccgugaccga gcggaucgag      360

aaggaggagu ucgcgcgcau ccugcuggcc aucaacaaga agcggggcua caagagcucc      420

cgcaaggcca agaccgagga cgaggggcag gccaucgacg gcauggcgau cgccaagcgg      480

cucuacgacg agaaccugac cccggggcag cugagccucc agcugcugca gcagaacaag      540

aagcuccugc ccgacuucua ccgcuccgac cugcagaagg aguucgaccu cgucuggaac      600

uuccagaagc aguucuaccc cgacauccug acggacaucu ucuacaagga gcugcagggc      660

aaggggaagg acgccaccag caaggccuuc uccaagcggu accacuucga caccgccgag      720

aacaagggca gcaaggaguc cgugcgccuc caggcguacc aguggcgggc cgaggccgug      780

agcaagcagc uguccaagga ggaggucgcc uacgugcuga ccgagaucaa caacaaccuc      840

aacaacgcca gcggcuaccu gggggcgauc uccgaccgca gcaaggagcu guacuucaac      900

cggcagacgg ugggccagua ccucuacgcc cagcugcagg agaaccgcca caacagccug      960

aagaacaagg ucuucuaccg gcaggacuac cucgacgagu ucgagcgcau cugggagacc     1020

caggcccugu uccacaagga gcugaccgac gagcucaaga agcagauccg ggacguggug     1080

aucuucuacc agcgcaagcc caagucccag aaggggcuga ucagcuucug cgaguucgag     1140

uccaaggaga ucgagaucga gaaggacggc aagaccauca cgaagaacau cggcgcccgc     1200

gucgugccca agagcucccc gcuguuccag gaguucaaga ucuggcagau ccucaacaac     1260

gugaucugca agcggaaggg gauccgcaag aagaagauca gcgccaagac cacccagcug     1320

gaccugcuca acgaguccag ccagaccauc uucucccugg acauggagug caagcagcug     1380

cuguucgacg agcugaaccu gaagggcgac cucaagagcg acaagguccu caagcugcug     1440

ggguacagcc cccaggagug ggagaucaac uacaaccagc ucgagggcaa ccggacgcag     1500

aaggcgcugu acgaggccua ccugaagauc guggagaugg aggcccacga cgugaaggac     1560

auccuccaga ucaagagcac caaggacgac uggucccugg acgagagccc ccuguccgcc     1620

agcgagaucc gcgaguccgu caaggccauc uuccaggcgc ucgggaucug caccaagauc     1680

cuguacuucg acccgcugcu ccccgugaag gacuucgagg agcaggacag cuaccagcug     1740

uggcaccucc ucuacuccua cgagagcgac gacagcaccu ccggcaacga gacgcuguac     1800

cggauccugg agaagaagua cgccuucaag cgcgagcacg cccggauccu cgccaacgug     1860

gcgcugcagg acgacuacgg cagccugucc accaaggcca uccgcaagau cuaccccaac     1920

aucaaggaga accaguacag caccgccugc gagauggccg gguacaagca cuccaagcuc     1980

agccugacca cggaggagcu ggaggcccgg gagcucaaga acaucauccc ccugcugaag     2040

aagaacgcgc uccgcaaccc ggucguggag aagauccuga accagaugau caacgugguc     2100

aacgcccuga ucgagaagaa cuccgagcgg gacgccgagg gcaagaucac caaguacuuc     2160

cacuucgacg agauccgcau cgagcucgcc cgggagcuga agaagaacgc ccagaagcgc     2220

uacgagauga cccagaacau caacaaggcg aaguccgagc accagaagau cuccgagauc     2280

cugcagaagg aguucgggau caagaacccc accaagagcg acaucauccg guaccgccuc     2340

uacaaggagc uggagcacaa cggcuacaag gagcuguaca cgaacaccua caucgccccc     2400

gacgugcugu ucuccaagaa caucgacauc gagcacaucg uccccaaggc ccggguguuc     2460

gacgacagcu ucuccaacaa gacccuggcc uucaaggacu ucaacaucaa gaagggggag     2520

agcaccgccu ucgacuacau caccucccug aagaacgagg agaagcucaa ccaguaccug     2580

gcgcgcgugg agaacgccua caagacgaag aacaucagcc cggccaagua caagaaccug     2640

cucaagaagg ccuccgagau cggcgacggc uucaucaacc gggaccugcg cgacacccag     2700

uacaucgcga agaaggccaa ggagauccug uuccagguca ccaagaacgu gcucagcacc     2760

agcgggucca ucacggaccg ccugcgggag gacuggagcc ugguggacgu caugaaggag     2820

cucaacaugc ccaaguacca gucccugggc cugaccgagg uggaggagcg caaggacggg     2880

aacaagguga ccgucaucaa ggacuggacc aagcggaacg accaccgcca ccacgccaug     2940

gacgcccuca cgguggccuu caccaagccc agcuacaucc aguaccugaa ccaccugaac     3000

gcgcggaagg acgagaacaa caagaacuac uccgugaucc ucgccaucga gaagaaggag     3060

accaucaagg uccccaccaa caacggcaag aacaagcgcg uguucaucga gccgaucccc     3120

aacuuccgcc agguggccaa gaagcaccug gaggagaucu ucaucagcca caaggccaag     3180

aacaaggucg ugacgaagaa caccaacaag cccgccggga ccaacaagca gcagaucacc     3240

cugacgcccc ggggccagcu ccacaaggag accaucuacg gcaaguacca guacuacgug     3300

aacaaggagg agaaggucgg ggugaaguuc gacaaggaga ccaucgcgaa gguguccaac     3360

ccgcuguacc gggaggcccu gcucaagcgc cugcaggcca acgacaacga ccccaagaag     3420

gccuucaccg gcaagaacgc ccugagcaag aaccccaucu accucgacga ggcgaagacg     3480

aagacccugc ccgagaaggu caaccugacc uaccucgagg aggacuucuc cgugcggaag     3540

aagaucgugc cgaagaaccc caccagcaag gaguucgucc ccaaccugaa guccaucgag     3600

aagguggugg acgagggggu ccgcaacauc cugaucgagc ggcuccgcgu guacaacaac     3660

gacccggaga aggccuucgu ggaccuggag aagaacccca ucuggcugaa caaggagaag     3720

ggcaucgcca ucaagcgggu cacgaucagc ggcgugaaca acgcccagcc ccuccacauc     3780

gggaaggacc accugggcaa gaccacccug aacaaggagg ggaaggagau ccccguggac     3840

uacgucucca ccggcaacaa ccaccacgug gcgaucuacc gcgacaagga ggggaaccuc     3900

caggagcaga ucgugagcuu cuucgaggcc gucgugcgga cgcagcaggg ccugccgauc     3960

aucgacaaga ccuacaagca ggaggagggc uggcaguucc uguucaccau gaagcagaac     4020

gagauguucg uguuccccaa cgaggccacc ggguucaacc ccgccgagau cgaccuccug     4080

gaccccaaga acaagaagca gaucuccccg aaccuguucc gcguccagaa gaucgccacg     4140

aaggacuacu ucuuccggca ccaccucgag accaacgugg agaccgacaa cgugcugaag     4200

aacaccacgu ggaagcgcga gggccugagc gggcucaagg acaucgucaa ggugcggauc     4260

aaccaccugg gcgacaucgu guccaucggg gaguacuga                            4299


<210>  2193
<211>  4302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2193
augaagaaga uccugggccu ggaccugggg accaccagca ucggcuacgc ccaggugaac       60

gaguccgagg acuucaagga cagcuccauc gagcgcaucg ggguccgggu gaacccccug      120

agcguggacg agcagacgaa cuucgagaag ggcaagccga ucuccaucaa cgccgaccgc      180

acccucaaga gcggggcgcg gcgcacccug gaccgguacc aggaccgccg guccaaccug      240

aucgacgccc ucgucaagag cgugaucauc accgaggaca cggugcuggc cgaggacggc      300

aagaacacca cccacuccac cuucgcccug cgcgccaaga gcgcguccga gaaggucgag      360

aaggccgagu ucgcccggau ccuccuggcc aucaacaaga agcgcggcua caagagcucc      420

cggaagacga acaacaacga cgacgagggg caggcgaucg acggcauggc caucgccaag      480

aagcuguacg acgagaaccu cacccccggg cagcuggccu accagcugcu caaggagggc      540

aagaaguacc ugcccgacuu cuaccgcagc gaccugcagg ccgaguucga caagaucugg      600

accuuccaga agcaguucua ccccgagauc cucaccgacg agcugcagaa ggagcuggcg      660

gggaagggcc agcgggccac guccgccauc uucuggaaga aguacgacuu caacaccgcc      720

gagaacaagg gcacccgcga ggagaagaag cucaaggccu acgaguggcg gagcaccucc      780

guggacaaga agcugcagaa ggaggaggug gcguacguca ucacggagau caacaacaac      840

cugaccaaca gcuccgggua ccucggcgcc aucagcgacc gcagcaagga gcuguacuuc      900

aacaagcaga ccguggggca guaccuguuc aagcagaucc agaagaaccc gcacaccaag      960

cucaagaacc agguguucua ccggcaggac uacuucgacg aguucgagau caucugggag     1020

acgcaggcca aguuccaccc cgagcugacc gaggagcuga agaacaagau ccgcgacauc     1080

aucaucuucu accagcggaa gcucaagucc cagaagggcc ugaucagcuu cugcgaguuc     1140

gagcgcaagg aggucgagau cgagaagggc gggcggcgca ugaagaagac caucggccug     1200

aagguggccc ccaaguccag cccccucuuc caggaguuca agaucuggca gguccugaac     1260

aacgugcuga uccgcaagaa gggguccaag aagcggcgcg ugaaggagcc ggaccagcuc     1320

aaccuggugg agcaggagca ggaggucaug uucuucgaca ugcagaccaa gcagacgcug     1380

uucgacgagc ucaacgugaa gggcaaccug aaggccaagc ggaucaucga gcugaucggg     1440

caggacgcga acgacuggga gaucaacuac agcgagaucg agggcaaccg caccaacaag     1500

gcccucuacg acgccuaccu gaagauccug gaccucgagg gcuacgacac caaggcccug     1560

cuggacguga agaccuccaa ggacgagguc gagcucgacg acaucaacau cagcgggccc     1620

gaggugaagg accuggugaa gcggaucuuc gagucccugg agaucgacac gcagauccug     1680

gaguucgacg ccgagcucga cggcgagguc uucgagaagc agagcuccua ccugcugugg     1740

caccuccugu acagcuacga gggggacgac ucccccagcg gcaacgagaa gcuguacaag     1800

cuccugaagc agaaguucgg guucaagaag gagcacgcgc agauccuggc cuccaucacc     1860

cucagcgacg acuacggcuc ccugagcgcc aaggccaugc gcaagaucua ccccuacauc     1920

cgggagcaga ccuucgacaa ggcggccucc cuggccggcu accgccacag cgccagcucc     1980

cucaccaagg aggagaucgc caaccggccg cugaaggaca agcuggagcu caucaagaag     2040

aacagccugc gcaaccccgu ggucgagaag auccugaacc agguggugaa caucgucaac     2100

ucccucaucg aggcgaacag cgagaaggac uccgacggga acaucaucag cuacuucaag     2160

uucgacgaga uccggaucga gcuggcccgc gaccugaaga agaacgccaa ggagcgggcc     2220

gagaugacgc ucaacaucaa cgccgcgaag aucgugcacg agaagaucuu caagcugcug     2280

caggccgagu ucggcgugaa gaaccccacc cgcaacgaca ucauccggua caagcucuac     2340

gaggagcuga agaacaacgg guacaaggac cuguacacca acaucuacau cccccgcgag     2400

auccucuucu ccaagcagau cgacaucgag cacaucaucc cgcagagcaa gauguucgac     2460

gacuccuuca gcaacaagac caucgucuuc cggaaggaca accuggacaa gggcaacucc     2520

acggccuucg acuacaucga cagccgcuuc gggaaggaca agcugaccga cuucgagcag     2580

cggaucgagu uccucuucga gcagggcaag aagaacaagg aggagggcau cuccaaggcc     2640

aaguaccaga agcugcugaa gcgcgaggcc gacaucgggg acggcuucau cgagcgcgac     2700

cuccgggaca cccaguacau cgcgaagaag gcccgcaacc ugcuguacga gaucugccgg     2760

aucgugaccc ccaccacggg gagcaucacc gaccgccucc gggaggacug ggaccuggug     2820

aacaucaugc aggagcugaa ccucgacaag uucaagaagc ugggccugac cgagaugguc     2880

gagaagaagg acggcuccuu caaggagcgc aucguggacu ggagcaagcg gaacgaccac     2940

cgccaccacg ccauggacgc ccucaccauc gcguucacga agcacuccca cauccaguac     3000

cugaacuacc ugaacgcccg gaagaacgag aaccacaagc agcaccacaa caucaucggg     3060

aucgagcgca aggagaccga guucgugauc gacgaccucg gcaacaagaa gcggaaguuc     3120

caccuccccc ugcccaacuu ccgcgaggag gccaagaagc accuggagaa cguccuggug     3180

agcuucaagg ccaagaacaa gguggucacc aagaacaaga acaagaccgc cagcuccaag     3240

ggggagaaga ucaaggugga gcugacgccg cggggccagc uccacaagga gaccguguac     3300

gggaaguaca aguucuacgu cagcaaggag gagcgcaucg gcggcaaguu cgaccagcgg     3360

accaucgagc gcguggcgaa cuccacccac aagcggcugc ugcuccagcg ccugcuggag     3420

aacgacaacg acgccaagaa ggccuucacg gggaagaacg cccucgccaa gaaccccauc     3480

uaccuggacg acaccaagac ccugacccuc cccgagaucg ugaagcugac guggcuggag     3540

gaggacuaca gcauccggaa ggacaucacc cccgagaacu ucaaggacau caaggucauc     3600

gacaagaucc ucgacgaggg cgugaagcgc auccugaagg agcggcuggc gaagcacaac     3660

aacaacccga aggaggccuu cuccgaccuc gagaagaacc ccaucuggcu gaacgagaag     3720

aaguccauca gcaucaagaa ggugaagauc agcgggauca acaacgccga ggcccugcac     3780

uacaagaaga accaccucgg caucgagauc cuggacgacg agaagaagcg cauccccguc     3840

gacuucgugu ccaccgggaa caaccaccac guggccaucu accgcgacga gaagggcaac     3900

cugcaggagg acgucgugag cuucuucgag gcgguggccc gggucaacca gaaccucccc     3960

aucaucgaca agacccugaa ggccuaccug ggcuggacgu uccucuucac caugaagcag     4020

aacgaguacu ucaucuuccc gaacaagcag accggguuca accccaccga gaucgaccug     4080

cuggacucca ugaacaacaa gcucaucagc cccaaccugu uccgcgugca gaaguucacg     4140

gugaaggacu acuucuuccg gcaccaccug gagaccaacg ucgaggacga cuccaagcuc     4200

aagaacacca ccuggaagcg cgagggccuc gggggcaucg ggaacaucgu gaaggugcgg     4260

aucaaccaca ucggccagau cgucaagauc ggcgaguacu ga                        4302


<210>  2194
<211>  4305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2194
augaagaaca uccugggccu cgaccugggg accaacagca ucggcugggc cuacgugaag       60

caggcccaga acgacuccga gaaguccgag aucgcggagc uggggguccg cgugaacccc      120

cucaccgugg acgagaagac gaacuucgag aagggcaagc cgaucagcac caacgccgac      180

cggacccuga agcgcggggc ccggcgcaac cugcagcggu acaagcuccg ccggcaggag      240

cugaucgagc ugcucaagga cuaccagguc aucagcgaca agaccauccu ggccgaggac      300

ggcaaggaga cgaccuucga gacccugcgc cuccggguga aguccgccaa ggagcagguc      360

agccuggagg agcuggcgcg cauccuccug augaucaaca agaagcgggg cuacaagucc      420

agccgcaagg ccaagaacga ggacgagggg cagcugaucg acggcaugga gaucgcccgg      480

cgccucuacg acgagaaccu gaccacgggg cagcuguccc uccagcugcu gaaggagggc      540

aagaaguccc uccccgacua cuaccggagc gaccugcagc acgagcugga caaggugugg      600

aacuuccaga agcaguucca ccccgacauc cucaccgacg aguucuacac cagcauccag      660

gggaagggcc agaaggccac ccgcgagucc uucagcucca aguacggcau cgacacggcg      720

gagaacaagg ggagccggga ggagaagaag cugcaggccu accaguggcg cgccgacgcc      780

acccacaagg agcugaccaa ggaggagcuc gccuucguga ucaccgagau caacaacaac      840

aucaacaacu ccagcggcua ccugggggcg aucuccgacc ggagcaagga gcugaucuuc      900

gccaacgaga cggugggcga guaccucugg aagcagaucc agaacgaccc ccacaccagc      960

cugaagaacc aggucuucua ccgccaggac uacuucgacg aguucgagcg gaucugggag     1020

acccaguccg gcuucuaccc gcagauccug accgagagcc ucaagaacga gcugcgcgac     1080

auccugaucu ucuaccagcg caagcucaag ucccagaagg cccuggugag caucugcgag     1140

uucgagucca aggagauccc ccugaaggac aagcagggga accccaucag caagaacggc     1200

auccccguga cgaagcucgu cgggccgaag guggccccca aguccagccc ccuguuccag     1260

gaguucaaga ucuggcagaa ccugaacaac gugcucgucc ggcccaaggg cucccgcaag     1320

cggaagguga ccaccaacca gcagagcacc cugcuggagg ggcaggagcc gguguucgag     1380

cucaaccugg agcagaagga gcugcuguuc gaggagcuca accugaaggg caaccugaag     1440

acggacuacu gccucgagcu gaucggcuac uccgccaagg agugggagau gaacuacccc     1500

acccuggagg ggaacaagac caacaaggcg cucuacgagg ccuaccugaa gaucaucgac     1560

agcgagggcu acgacgccaa gaagcugcuc aaggucaaga ccgacaagga cgacaucaac     1620

cuggacgaca ucaagguguc cgccagcgag aucaagacga ugaucuccga caucuucaac     1680

gagcugggga ucaacaccaa gauccucgac uucgacgccg agcuggagcg caagcaguuc     1740

gaggcgcagg ccagcuaccg gcuguggcac cuccuguacu ccuacgaggg cgacgacagc     1800

aaguccggga acgagcugcu cuaccgccug cuggaggaca aguucggcuu caagcgggag     1860

cacagcaaga cccucgccaa cgugagccug cuguccgacu acggcaaccu cagcaccaag     1920

gccauccgca cgaucuaccc cuacaucaag gagaacaagu ucuccaccgc cugcgagcug     1980

gcgggguacc ggcacagcaa gcuguccauc agcaaggagg agaacgagaa ccgcacccuc     2040

aaggacaccc uggacguccu gaagaagaac ucccuccggc agcccguggu ggagaagauc     2100

cugaaccaga ugaucaaccu gaucaacgag cucaucgaga agcacagcga gaaggacgag     2160

aagggcaaca ucgucaagua cuucaaguuc gacgagaucc acaucgagcu ggcccgcgag     2220

cugaagaaga acgccgagga gcggcgcgag cucgacucca aggugcggga ggggaaggac     2280

cgcaacgaga agaucaucac gauccucaag agcgaguucg gccugccgua ccccaccaag     2340

aacgacauca cccgguaccg ccucuacgag gagcugaagg acaacgggua caaggaccug     2400

uacaccaaca ccuacauccc ccgggagaag cuguucucca aggagaucga caucgagcac     2460

aucaucccca agagccgccu guucaacgac uccuucagca acaagacgau cugcuaccgc     2520

caggacaacc ugaacaaggg cgagcggacc gccuacgacu acaucgaggg ccucgggaag     2580

acccagcugg acgaguaccu gaaccgcguc gagaaccucu acaccaacaa guucaucacg     2640

aaggcgaagu accggaaccu gcugaagaag gaguccgaga ucggcgacgg guucaucgag     2700

cgcgaccucc gggagaccca guacaucgcc aagaaggcca aggagaugcu gcugcagauc     2760

acccgccacg uggugagcac cacgggcucc aucaccgacc ggcuccgcga cgacugggag     2820

cuggucaacg ugaugcggga gcugaaccuc ccgaaguaca aggugcuggg ccugaccgag     2880

auccaggagc gcaagaacgg gcagaccguc gaggugauca cggacuggac caagcggaac     2940

gaccaccgcc accacgccau ggacgcccuc accguggcgu ucaccaagca cagccacauc     3000

caguaccuga acuuccugaa cgcccggaag gacgagaagc acaaggagua cagcaacauc     3060

augggcaucc agcagcucga gacggagaag accaccgaca aggacgggaa ggaccgccgg     3120

gucuucaaga cccccauccc caacuuccgc cagguggcca aggagcaccu ggaguccauc     3180

cugaucagcc acaagacgaa gaacaaggug gucacccggg ccaccaacaa gaucgccggc     3240

uccaagaagc agcaggagac ccucagcccc cgcgggcagc ugcacaagga gacgguguac     3300

ggcaaguacc ggcgcuacgu gcagaaggag gagaagaucg gcuccaaguu cgaccugcag     3360

accaucgagc gggucgcgaa cccgcucuac aagcgccugc ugaccaagcg ccucgaggag     3420

aacgggggcg accccaagaa ggccuucacc gggaagaacg cccuggccaa gagccccauc     3480

uaccuggacg agacgcagac ccagcagcuc cccgagaagg ugaagcuggu guggcuggag     3540

accgacuucu ccauccggaa ggacaucacc ccggacaacu ucaaggacga gaaguccauc     3600

gacaaggucc ucgacgaggg caucaagcgc auccugaagg accggcucaa gagcuucgac     3660

ggggacgcca agcgcgcguu cacggaccug gagcagaacc ccaucuggcu gaacaaggag     3720

aagggcaucg ccaucaagcg ggugaccauc agcggcgugu ccaacgccga gccccugcac     3780

accaagaagg accaccucgg gaacgagauc uucgacgaca ccggcaagcc gauccccguc     3840

gacuacguga gcacggggaa caaccaccac aucgccaucu accgcgacga gaacggcaac     3900

cugcaggaga aggcgguguc cuucuucgag gccgucaccc gggccaacca ggggcugccc     3960

accguggaca agaccuacaa ccagcaccuc ggcuggcagu uccuguucac caugaagcag     4020

aacgagaugu ucguguuccc caacaaggag acgggcuucg acccgcagga gaucgaccug     4080

cucgacccca agaacaagaa gcugaucagc cccaaccugu uccgcgucca gaagaucucc     4140

accaagaacu acuucuuccg gcaccaccuc gagaccagcg uggagaccca caacgcccug     4200

aacgggguga cguacaaguc ccagcugggc cugaacggga ucaccgacau caucaagguc     4260

cgcaucaacc accucggcga caucgugcac aucggggagu acuga                     4305


<210>  2195
<211>  4308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2195
augcugggcu gcgagcgcaa gcggaagcgc cgggagaagg agaagauggc caagcccaag       60

gacuacacca ucgggcucga caucggcacc aacagcgugg ggugggucgu gacggacgac      120

cagaacaaca uccugcgcau caagggcaag aaggccaucg gggcgcggcu guucaccgag      180

ggcaaggugg ccgccgagcg ccgguccuuc cgcaccaccc ggcgccggcu cagccgccgg      240

cgcuggcgga ucaagaugcu ggaggagcug uucgacgagg agaucgccaa ggucgacccg      300

uccuucuucg cccgccucca cgagagcugg aucuccccca aggacaagcg gaagcgcuac      360

agcgcgaucg uguuccccuc ccccgaggag gacaagaagu uccacgagag cuacccgacg      420

aucuaccacc uccgggacaa gcugaugaag gacgaccaga agcacgacau ccgcgagauc      480

uacaucgccg ugcaccagau gaucaaggcc cggggcaacu uccugcacga cgaguccguc      540

gagaccuacc gcagcgggau guccagccug ggcgggcgcu ccgagcggaa cauccugagc      600

gugcagaccc ucgaggagcu gaacgaccug uucgccgaga acgagggcac cgaggaggug      660

gagcucaacg ucgcguccgc cgagcagauc aacgacaucc ugaccggggg ccaccugaac      720

gccgacagcc agaaggagau cuccaaccuc cugcugccca gcuccuuccc cagcuucgac      780

gacaaggcca aggagaagca ggucaagaag cucaucaaca acguggccac gaacaucagc      840

aaggcguggc ugggcuacaa ggccgacuuc uccaccaucc ugaaccucgc caagguggac      900

aaggaccaga agaagaucuu cgccuucgcc cugcagggcg gggacgagga ggacaaggug      960

caggagcugg agagccuccu ggagcagucc cagaccgaca ucguggaccg ccugaucgag     1020

auccggcacg cgaucguccu cagcgagauc gugcccgugg ggaugacgcu guccgaggcc     1080

augaucgaca aguacgacca gcacaaggag gaccugauca cccucaaggc cgucauccgc     1140

aacaccaagg acaagaagaa ggccgccaag cugcaggcga ucuacgaccu guacgugaag     1200

aaccggcacg ccgaccucgc caaggccaug aagcugaccg gcaucaagaa gcgcagcgag     1260

cugcucgacc cggaggagcu gaagaagggc aucuccagcc ugcucgacga cucccccgag     1320

gccguggaga ucaagcagcg gcuggaggag aagacguucc ugccccucca gcgcagcaac     1380

aacaacgggg ucauccccaa ccagcugcac cagguggagc uggacgagau caucaagaag     1440

caguccaagu acuacccguu ccucgcggag aagaaccccg acgagagcga ggaggcccag     1500

aagaaggccc ccaccaagcu ggacgcccug cugaccuucc gggugcccua cuacgucggc     1560

ccgaugauca ccaaggagga gcaggaggcg cagaacgggc acuccuucgc cuggauggug     1620

cgccgggacc cccaggacca cgaggccauc acgcccugga acuucgagaa gaaggucgac     1680

aagauggcca gcgccaccca guucaucaag cgcaugacca ccaaggacac guaccuccug     1740

ggcgaggacg ugcugcccgc guccagccuc aaguaccagc uguucaccgu gcugaacgag     1800

cucaacaacc ugcgggucaa cgggaagaag cugaccuccg acgagaagga gcaggucauc     1860

gagggccucu ucaagaagca gaagaccgug aaggccgaca aguucgugaa guacuggcag     1920

gccaagcaca ucggcgccga caucaaggug aaggggcuga gcgacccguc caaguucaac     1980

agcacgauga gcaccuacau cgacuucaag aagaucuucg gcgaccagcu gaacgacgug     2040

aaccgccaga acgacuucga gaagaucauc gaguacucca ccaucuucga ggaccggaag     2100

auccucgagg acaagcugcg cgucgacuuc gccuggcuga gcgaggagca gaucaagcag     2160

cucugccggc ugcgccugca gggguggggc cggcucuccg acaagcugcu gacccgccuc     2220

acggacgcgg acgggcagaa cgugcuggag cgccugugga acagcaacga caacuucgug     2280

cagaucgucu ccgaccccaa caucaaggcc aagaucgagc aggagaacca gaagcuccug     2340

cgggacagcc aguccgacaa cgccguggag agcauccugg acgacgccua caccagcccc     2400

cagaacaaga aggccauccg ccaggugaug cgggucgugc acgacaucgu gaaggcgugc     2460

gacggcaacg uccccgccaa guucgccauc gaguucgccc gcagccagga ggacgacccg     2520

aagcggacca ccgcgcgcgu gaaccagaug cagaagaucu acgaccagau cuccgacgag     2580

guggucagcc agggcgugaa ggagcagcuc uccgggauga agucccugaa ggaccgguac     2640

uaccuguacu ucaugcaggg cgggcgcgac gccuacacgg gcgccaagcu caacaucgac     2700

cggcuguccg acuacgacgu ggaccacauc augccccaga gcuucgucaa ggacgacucc     2760

cuggacaacc gcgugcucgu ggcccgggcc gucaacaacc agaaguccga caaggugccc     2820

gcgcugcugu ucgggaacaa gguggucgcc gaccucggca ucaccgugcg cgagaugugg     2880

gacaaguggc agaagcuggg caugaucagc aagcggaagc ugagcaaccu ccugaccgac     2940

agcgacgccc ugaccgagua ccgcgcccag ggguucaucc ggcgccagcu cguggagacc     3000

ucccagguca ucaagcugac ggccaccauc cugcagagcg aguuccccga cuccaagauc     3060

aucgaggugc cggcgaagua caacagcauc gugcggaagc aguucgaccu cuacaagucc     3120

cgcgagguca acgacuucca ccacgccauc gacgccuacc ugagcaccau cgugggcaac     3180

uaccuguacc agguguaccc caaccuccgg cgcauguucg ucuacgggga guucaagaag     3240

uucuccagca acgccgagga guccgcccac gacguggcgc ggcgcgucaa gagcaugaac     3300

uuccuggacg accugcuccg cggcacccac ggcgacaaca ucuacugccg guccacgggg     3360

gagaucgugu ucaaccgcaa cgacaucauc agcaagcuga agcaggccua cuccuucaag     3420

cagaugcugg ugacccagga gguguucacc aagaaguccg cccucuucaa ccagaccguc     3480

uaccccagcc cgaagcggga cggcagcaag cgcuccgggc ugaucccccg gaagcgcggc     3540

auggaccccg agaucuacgg gggcuacgcc cacaaccagg acagcuacuu cgugcuggcg     3600

aagaucgaga aggugaaggg cuacacgcuc cagaucgucg gggugcccau ccggagccug     3660

gagaucauca acaaggccga ggacuucucc accgcccugc ucgacaucau caagagcaag     3720

gugauguaca acgagaagac cggccagccg aagcgcggga ucaagaacgu cgagauccug     3780

gugaacaagc ugcccuaccg gcagcccauc cucgagaagg acgucuccua caugcugggc     3840

agcuccgccu acaaguacaa caucaagcag aucacccuga gccgccaguc caugaaguac     3900

auccucgacu acaucgacga ccccaacuuc acgaagcacg agaugaccau gaucgacgag     3960

cagaacgagc gggagugccu gcugagcgug uacgacgaga uccucgagaa gguggacaag     4020

uaccugccgc uguucgacgg gaacaaguuc cgcgagaagc uccacuccgc ccggaaggag     4080

uucgucagcc uguccgugga cgacgaggcg agcgccaucg agaagauccu gcgcggccuc     4140

cacgccaacu ccgagaugcc cagccuggag aaccugaaga ucaagcccgc ccucggcuuc     4200

uuccagauga agaacgggau cacccugucc aacgacgccg ugcugcucua ccagagcccc     4260

accggccugu ucgagcggcg cgucaaggug uccgaccugc ucaaguga                  4308


<210>  2196
<211>  4308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2196
augaagaaca uccugggccu cgaccugggg accaccagca ucggcuucgc ccacaucgug       60

gaggacgaga acaaggagaa guccgagauc aaggagcugg ggguccgcau cgugccccuc      120

acgaccgacg agcagagcga cuucgagaag ggcaagucca ucaccaccaa cgccaaccgg      180

acgcugaagc acggggcgcg ccugaaccuc gaccgguacc agcagcgccg gaaguaccug      240

aucgaccugc uccagaaggc caaccugauc accagcucca gcauccuggc cgagaacggc      300

aagaacacca cccacuccac guggcagcuc cgcgccaagg ccgugaccga gcggaucgag      360

aaggaggagu ucgcgcgcgu ccugcuggcc aucaacaaga agcggggcua caagagcucc      420

cgcaaggcca agaccgagga cgaggggcag gccaucgacg gcauggcgau cgccaagcgg      480

cucuacgacg agaaccugac cacggggcag cugagccucc agcugcugca gcagaacaag      540

aagcuccugc cggacuucua ccgcuccgac cugcagaagg aguucgaccu cguguggaac      600

uuccagaagc aguucuaccc cgacauccug accgacagcu ucuacaagga gcugcagggc      660

aaggggaagg acgccaccuc caaggccuuc agcaagcggu accacuucga caccgccgag      720

aacaagggcu ccaaggagag cgugcgccuc caggcguacc aguggcgggu cgaggccauc      780

uccaagcagc ugagcaagga ggagguggcc uacgugcuga cggagaucaa caacaaccuc      840

aacaacgccu ccggcuaccu gggggccauc agcgaccgca gcaaggagcu guacuucaac      900

cggcagaccg ucggccagua ccucuacgcg cagcugcagg agaaccgcca caacucccug      960

aagaacaagg uguucuaccg gcaggacuac cucgacgagu ucgagcgcau cugggagacc     1020

caggccagcu uccacaagga gcugaccgag gagcugaaga agcagauccg ggacgugguc     1080

aucuucuacc agcgcaagcc caagucccag aaggggcuca ucagcuucug cgaguucgag     1140

uccaaggaga ucgagaucga gaaggacggc aagacgauca ccaagaccau cggcgcccgc     1200

guggugccca agagcucccc gcuguuccag gaguucaaga ucuggcagau ccugaacaac     1260

gucaucugca agcggaaggg gauccgcaag aagaagguga gcgccaagac cacgcagcuc     1320

gaccugcuga acgaguccag ccagaccauc uucucccucg acauggagug caagcagcug     1380

cuguucgagg agcucaaccu gaagggcgac cugaaguccg acaaggugcu gaagcuccug     1440

ggguacagcc cccaggagug ggagaucaac uacaaccagc uggagggcaa ccggacccag     1500

aaggcccucu acgaggcgua ccugaagauc gucgagaugg aggcccacga cgugaaggac     1560

auccugcaga ucaagagcgc caaggacgac uggucccucg acgagagccc ccuguccgcc     1620

agcgagaucc gcgaguccgu gaaggccauc uuccaggcgc uggggaucug caccaagauc     1680

cucuacuucg acccccugcu gccggucaag gaguucgaga agcaggacag cuaccagcuc     1740

uggcaccucc uguacagcua cgaguccgac gacagcacgu ccggcaacga gacccucuac     1800

cggauccugg agaagaagua cgccuucaag cgcgagcacg ugcggauccu ggccaacgug     1860

gcccuccagg acgacuacgg cagccugucc accaaggcga uccgcaagau cuaccccaac     1920

aucaaggaga accaguacag caucgccugc gagauggccg gguacaagca cuccaagcug     1980

agccucacca cggaggagcu ggaggcccgg gagcugaaga acaucauccc ccuccugaag     2040

aagaacgccc ugcgcaaccc cgucguggag aagauccuca accagaugau caacgucgug     2100

aacgcgcuga ucgagaagaa cuccgagcgg gacgccgagg gcaagaucac caaguacuuc     2160

cacuucgacg agauccgcau cgagcuggcc cgggagcuca agaagaacgc ccagaagcgc     2220

uacgagauga cccagaacau caacaaggcc aagcuggagc accagaagau cagcgagauc     2280

cugcagaagc aguucgggau caagaacccg accaaguccg acaucauccg guaccgccuc     2340

uaccaggagc uggagcacaa cggcuacaag gagcuguaca cgaacgcgua caucgccccc     2400

gacgugcugu ucagcaagaa caucgacauc gagcacaucg uccccaaggc ccggguguuc     2460

gacgacuccu ucagcaacaa gacccuggcc uuccgcgagu ucaacaucaa gaagggggag     2520

uccaccgccu ucgacuacau caccagccug aacuccgagg aggagcucaa ccaguaccug     2580

acccgggugg agaacgcgua caagaccaag agcaucagcc ccaccaagua caagaaccug     2640

cucaagaagg ccuccgagau cggcgacgac uucaucaacc gcgaccugcg cgacacccag     2700

uacaucgcca agaaggccaa ggagauccug uuccagguca ccaagaacgu gcucagcacg     2760

uccggcagca ucaccgaccg gcugcgcgag gacugggggc ugguggacgu caugaaggag     2820

cucaacaugc cgaaguacca gucccugggc cugaccgagg uggaggagcg gaaggacggg     2880

aacaagguga ccgucaucaa gaacuggacg aagcgcaacg accaccggca ccacgcgaug     2940

gacgcccuca ccguggccuu caccaagccc agcuacaucc aguaccugaa ccaccugaac     3000

gcccgcaagg acgagaacaa caagaacuac uccgugaucc ucgccaucga ggagaaggag     3060

accaucaagg uccggacgaa caacggcaag aacaagcgcg uguucaucga gcccaucccc     3120

aacuuccgcc agguggcgaa gaagcaccug gaggagaucu ucaucagcca caagaccaag     3180

aacaaggucg ugaccaagaa cgugaacaag ccggccggga ccgacaagca gcagaucacg     3240

cugacgcccc ggggccagcu ccacaaggag accaucuacg gcaaguacca guacuacguc     3300

aacaaggagg agaagaucgg ggugaaguuc gacaaggaga ccaucgccaa gguguccaac     3360

ccccuguacc gggaggcccu gcucaagcgc cugcaggcca acgacaacga ccccaagaag     3420

gcguucacgg gcaagaacgc ccugagcaag aagccgaucu accucgacga guccaagacc     3480

aagacccugc ccgagaaggu caaccugacc uaccucgagg aggacuucag cauccggaag     3540

gacaucuccc ccgacaacuu caaggaccuc aagagcaucg agaaggugau cgaccagggg     3600

gugaagcgca uccugaucaa gcggcugcag gccuacgaca acgaccccaa gaaggccuuc     3660

gucgaccugg agaagaaccc gaucuggcug aacaaggaga agggcaucgc caucaagcgc     3720

gugacgaucu ccggcgugaa caacgcgcag ccccuccaca ucgggaagga ccaccugggc     3780

aagaccaccc ugaacaagga ggggaaggag auccccgucg acuacgugag caccggcaac     3840

aaccaccacg uggccaucua ccgggacaag gaggggaacc uccaggagca gaucgucucc     3900

uucuucgacg ccguggugcg cgcccagcag ggccugccga ucaucgacaa gacguacaag     3960

caggcggagg gcuggcaguu cauguucacc augaagcaga acgagauguu cgucuucccc     4020

aacgagacca ccggguucaa ccccgccgag aucgaccugc ucgaccccaa gaacaagaag     4080

cugaucagcc cgaaccuguu ccgggugcag acgaucuccg uggucaagua cggcaacagc     4140

guggugcgcg acuucaaguu ccggcaccac cucgagacca cccugcagga cgacaagauc     4200

cugcagggga ucaccuucca gcagaucaag agccucgagc cccugcgcaa caucgucaag     4260

gugcgcauca accaccuggg cgacaucgug uccaucgggg aguacuga                  4308


<210>  2197
<211>  4308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2197
augaagaaca uccugggccu cgaccugggg accaccagca ucggcuucgc ccacaucgug       60

gaggacgaga acaaggagaa guccgagauc aaggagcugg ggguccgcau cgugagccuc      120

gccacggacg agcaguccga cuucgagaag ggcaagagca ucuccaccaa cgcgaaccgg      180

acccugaagc acggggcccg ccugaaccuc gaccgguacc agcagcgccg gaaguuccug      240

aucgaccugc uccagaaggc caaccugauc accagcgccu ccauccuggc cgagaacggc      300

aagaacacga cccacagcac cuggcagcuc cgcgcgaagg ccgugaccga gcggaucgag      360

aaggaggagu ucgcccgcgu ccugcuggcc aucaacaaga agcggggcua caagaacucc      420

cgcaagacga agaccgagga cgaggggcag gcgaucgacg gcauggccau cgccaagugc      480

cucuacgacg agaaccugac ccccgggcag cugagccucc agcugcugca gcagaacaag      540

aagcuccugc cggacuucua ccgguccgac cugcagaagg aguucgaccu cguguggaac      600

cugcagaagc aguucuaccc cgacauccug accgacagcu ucuacaagga gcuccagggc      660

aaggggaagg acgccacguc caaggccuuc agcaagcgcu accacuucga caccgcggag      720

aacaagggcu ccaaggagag cgugcggcug caggccuacc aguggcgcgc cgaggccauc      780

uccaagcagc ugagcaagga ggaggucgcc uacgugcuca ccgagaucaa caacaaccug      840

aacaacucca gcggcuaccu gggggcgauc agcgaccggu ccaaggagcu cuacuucaac      900

cgccagaccg ugggccagua ccuguacgcc cagcugcagg agaaccggca caacagccuc      960

aagaacaagg ucuucuaccg ccaggacuac cuggacgagu ucgagcggau cugggagacg     1020

caggccuccu uccacaagga gcugaccgac gagcucaaga agcagauccg cgacguggug     1080

aucuucuacc agcggaagcu caagagccag aaggggcugg ucuccuucug cgaguucgag     1140

agcaaggaga ucgagaucga gaaggacggc aaggccauca ccaaguccau cggcgcccgc     1200

guggugccca agagcagccc ccucuuccag gaguucaaga ucuggcagau ccugaacaac     1260

gucacgugca agcgcaaggg gauccggaag aagaaguccu ccgccaagag cacggagcug     1320

gaccucuuca acgagccguc ccagagcauc uucucccugg acauggagug caagcagcug     1380

cuguucgacg agcugaaccu gaagggcgac cucaagagcg acaaggugcu gaagcugcug     1440

ggguacagcc cccaggagug ggagaucaac uacaaccagc ucgagggcaa ccgcacccag     1500

aagucccugu acgaggcgua ccugaagauc aucgagaucg agggguacga ggugaaggac     1560

auccuccaga ucaaguccac caaggacgac uggagccugg acgagagccc ccuguccgcc     1620

agcgagaucc gggagcgcgu caaggugauc uuccagaccc ucggcaucug cacgaagauc     1680

cuguacuucg acccccugcu cccggugaag gaguucgagc agcaguacuc cuaccagcug     1740

uggcaccucc uguacagcua cgaguccgac gacagcaccu ccggcaacga gacccuguac     1800

cggauccugg agaagaagua cgccuucaag cgcgagcacg ccaccauccu cgcgaacguc     1860

gcccugcagg acgacuacgg gagccugucc acgaaggcca uccggaagau cuaccccuac     1920

aucaaggaga accaguacag caccgccugc gagcucgcgg gcuacaagca cuccaagcug     1980

agccugacca ccgaggagcu cgaggcccgc gagcugaaga acaucauccc ccugcucaag     2040

aagaacgccc ugcggaaccc cgugguggag aagauccuga accagaugau caacgucgug     2100

aacgcccuca ucgagaagaa cuccgagcgc gacgccgagg ggaagaucac gaaguacuuc     2160

cacuucgacg agauccggau cgagcuggcg cgcgagcuga agaagaacgc ccaggagcgg     2220

uacgagauga cccagaacau caacaaggcc aagcucgagc accagaagau cagcgagaag     2280

cugcagaagc aguucggcau caagaacccg accaaguccg acaucauccg cuaccggcug     2340

uaccaggagc ucgagcacaa cggguacaag gagcuguaca ccaacacgua caucgccccc     2400

gacguccugu ucagcaagaa caucgacauc gagcacaucg ugcccaaggc ccgcguguuc     2460

gacgacuccu ucagcaacaa gacccucgcg uucaaggagu ucaacaucaa gaagggcgag     2520

agcaccgccu ucgacuacau caccucccug aacugcgagg gcgagcugaa ccaguaccuc     2580

acccgggucg agaacgccua caagaccaag agcaucuccc ugaccaagua caagaaccug     2640

cucaagaagg ccagcgagau cggggacggc uucaucaacc gcgaccugcg ggacacccag     2700

uacaucgcca agaaggcgaa ggagauccug uuccagguga ccaagaacgu gcucuccacg     2760

agcgggucca ucaccgaccg ccugcgcgag gacuggggcc uggucgacgu gaugaaggag     2820

cucaacaugc ccaaguacca gagccugggg cugaccgagg uggaggagcg gaaggacggc     2880

aacaagguca ccgugaucaa ggacuggacg aagcgcaacg accaccggca ccacgccaug     2940

gacgcccuca ccguggccuu caccaagccg uccuacaucc aguaccugaa ccaccugaac     3000

gcgcgcaagg acgagaacaa caagaacuac agcgucaucc ucgccaucga gaagaaggag     3060

accaucaagg ugcccaucaa caacggcaag aacaagcggg uguucaucga gcccauccug     3120

aacuuccgcc aggucgccaa gaagcaccug gaggagaucu ucaucuccca caaggccaag     3180

aacaaggugg ugacgaagaa caucaacaag cccgccggga ccgacaagca gcagaucacc     3240

cucaccccgc ggggccagcu gcacaaggag acgaucuacg ggaaguacca guacuacguc     3300

aacaaggagg agaagguggg cgugaaguuc gacgagcgca ccaucaccaa ggucagcaac     3360

ccccuguacc gggaggcgcu ccugaagcgc cugcaggcca acgacaacga ccccaagaag     3420

gccuucgccg gcaagaacgc ccucuccaag aaccccaucu accuggacga ggcgaagacc     3480

aagacgcugc cggagaaggu gaaccucacc uaccuggagg aggacuucag cauccggaag     3540

gacaucuccc ccgacaacuu caaggaccuc aagagcaucg agaaggugau cgaccagggg     3600

gucaagcgca uccugaucaa gcggcugcag gccuacgaca acgaccccaa gaaggccuuc     3660

guggaccugg agaagaaccc caucuggcuc aacaaggaga agggcaucgc caucaagcgc     3720

gugaccaucu ccggggucag caacgcccag ccgcugcaca ucggcaagga ccaccugggg     3780

aagaccacgc ucaacaagga gggcaaggag gugcccgugg acuacgucuc caccggcaac     3840

aaccaccacg uggcgaucua ccgggacaag gaggggaacc ugcaggagca gaucgugagc     3900

uucuucgagg ccgucgugcg cgcccagcag ggccugccca ucaucgacaa gaacuacaag     3960

caggccgagg gguggcaguu cauguucacc augaagcaga acgagauguu cguguucccg     4020

aacgaggcga ccggcuucaa ccccgccgag aucgaccucc uggaccccaa gaacaagaag     4080

cugaucagcc ccaaccucuu ccggguccag acgaucucca ucgugaagua cgggaacagc     4140

gugguccgcg acuucaaguu ccggcaccac cuggagacca cccugcagga cgacaagauc     4200

cuccagggca ucaccuucca gcagaucaag ucccuggagc cgcugcgcaa caucgugaag     4260

gugcggauca accaccucgg cgacaucguc aacaucgggg aguacuga                  4308


<210>  2198
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2198
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga acaaggacga gcgguccagc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagcugac gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggacccuca agcgcgggau gcggcgcaac cugcagcggu acaagcugcg ccgggagacg      240

cucaccgagg ugcugaagga gcacaagcug aucaccgagg acaccauccu cagcgagaac      300

ggcaaccgca cgaccuucga gaccuaccgg cugcgcgcca aggccgugac cgaggagauc      360

ucccuggagg aguucgcccg gguccuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagggggu ggaggagggc acgcugaucg acgggaugga caucgcgcgc      480

gagcucuaca acaacaaccu gaccccgggc gagcugugcc uccagcugcu ggacgccggg      540

aagaaguucc uccccgacuu cuaccggagc gaccugcaga acgagcugga ccgcaucugg      600

gagaagcaga aggaguacua ccccgagauc cucaccgacg ugcugaagga ggagcugcgg      660

ggcaagaagc gcgacgccgu cugggccauc ugcgcgaagu acuucgugug gaaggagaac      720

uacaccgagu ggaacaagga gaagggcaag acggagcagc aggagcggga gcacaagcuc      780

gaggggaucu acuccaagcg caagcgcgac gaggccaagc gggagaaccu gcaguggcgc      840

gugaacggcc ugaaggagaa gcucagccug gagcagcugg ucaucguguu ccaggagaug      900

aacacccaga ucaacaacuc cagcggguac cucggcgcca ucuccgaccg gagcaaggag      960

cuguacuuca acaagcagac cgugggccag uaccagaugg agaugcugga caagaacccc     1020

aacgccuccc uccgcaacau ggucuucuac cggcaggacu accuggacga guucaacaug     1080

cugugggaga agcaggccgu guaccacaag gagcucaccg aggagcugaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cucaagagcc agaaggggcu gaucggcuuc     1200

ugcgaguucg agucccggca gaucgaggug gacaucgacg ggaagaagaa gaucaagacg     1260

gucggcaacc gcgugaucag ccgguccagc ccgcuguucc aggaguucaa gaucuggcag     1320

auccugaaca acaucgaggu gaccgucgug gggaagaagc gcaagcggcg caagcucaag     1380

gagaacuaca gcgcgcuguu cgaggagcug aacgacgccg agcagcugga gcucaacggc     1440

ucccggcgcc ugugccagga ggagaaggag cugcucgccc aggagcuguu cgugcgggac     1500

aagaugacca agagcgaggu ccugaagcuc cuguucgaca acccccagga gcuggaccuc     1560

aacuucaaga ccaucgacgg caacaagacg ggguacgccc uguuccaggc cuacuccaag     1620

augaucgaga ugagcggcca cgagcccgug gacuucaaga agcccgucga gaagguggug     1680

gaguacauca aggcggucuu cgaccugcuc aacuggaaca ccgacauccu gggguucaac     1740

uccaacgagg agcuggacaa ccagagcuac uacaagcucu ggcaccuccu guacuccuuc     1800

gagggcgaca acaccccgac cgggaacggc cgccugaucc agaagaugac ggagcuguac     1860

ggcuucgaga aggaguacgc caccauccug gccaacguga gcuuccagga cgacuacggg     1920

ucccucagcg ccaaggccau ccacaagauc cugccccacc ugaaggaggg caaccgguac     1980

gacguggcgu gcgucuacgc cggguaccgc cacuccgaga gcucccucac ccgcgaggag     2040

aucgccaaca aggugcugaa ggaccggcug augcuccugc ccaagaacag ccugcacaac     2100

cccguggucg agaagauccu caaccagaug gugaacguga ucaacgucau caucgacauc     2160

uacggcaagc cggacgagau ccgcguggag cuggcccggg agcugaagaa gaacgccaag     2220

gagcgcgagg agcucaccaa guccaucgcg cagacgacca aggcccacga ggaguacaag     2280

acccugcugc agaccgaguu cgggcucacg aacgugagcc ggaccgacau ccugcgcuac     2340

aagcuguaca aggagcucga guccugcggc uacaagaccc uguacagcaa caccuacauc     2400

ucccgggaga agcuguucag caaggaguuc gacaucgagc acaucauccc ccaggcccgc     2460

cucuucgacg acagcuucuc caacaagacc cuggaggccc ggagcgucaa caucgagaag     2520

ggcaacaaca cggcguacga cuucgugaag gagaaguucg gggaguccgg cgccgacaac     2580

agccuggagc aguaccucaa caacaucgag gaccuguuca aguccgggaa gaucagcaag     2640

accaaguaca acaagcugaa gauggccgag caggacaucc ccgacggcuu caucgagcgc     2700

gaccuccgga acacccagua caucgccaag aaggcccugu ccaugcugaa cgagaucagc     2760

caccgcgugg ucgcgaccuc cggcagcgug acggacaagc uccgggagga cuggcagcug     2820

aucgacguga ugaaggagcu gaacugggag aaguacaagg cccucgggcu ggucgaguac     2880

uucgaggacc gcgacggccg gcagaucggg cgcaucaagg acuggaccaa gcggaacgac     2940

caccgccacc acgccaugga cgcccugacc guggccuuca ccaaggacgu guucauccag     3000

uacuucaaca acaagaacgc gucccucgac cccaacgcca acgagcacgc caucaagaac     3060

aaguacuucc agaacggccg ggccaucgcc ccgaugcccc ugcgcgaguu ccgggcggag     3120

gccaagaagc accuggagaa cacgcucauc agcaucaagg ccaagaacaa ggucaucacc     3180

gggaacauca acaagacccg caagaagggc ggcgugaaca agaacaugca gcagacgccc     3240

cgcgggcagc ugcaccugga gaccaucuac ggcuccggga agcaguaccu caccaaggag     3300

gagaagguga acgccagcuu cgacaugcgg aagaucggca ccgucuccaa gagcgcguac     3360

cgcgacgccc ugcugaagcg gcucuacgag aacgacaacg accccaagaa ggccuucgcc     3420

gggaagaacu cccuggacaa gcagccgauc uggcuggaca aggagcagau gcgcaaggug     3480

cccgagaagg ugaagaucgu cacccucgag gccaucuaca cgauccggaa ggagaucagc     3540

cccgaccuga agguggacaa ggugaucgac gucggcgugc gcaagauccu gaucgaccgg     3600

cucaacgagu acggcaacga cgcgaagaag gccuucagca accuggacga gaaccccauc     3660

uggcugaaca aggagaaggg gaucuccauc aagcgcguga ccaucagcgg caucuccaac     3720

gcccagagcc uccacgucaa gaaggacaag gacgggaagc cgauccugga cgagaacggc     3780

cggaacaucc ccguggacuu cgugaacacc gggaacaacc accacgucgc cguguacuac     3840

cgccccguga ucgacaagcg gggccagcug gucguggacg aggccggcaa ccccaaguac     3900

gagcucgagg agguggucgu guccuucuuc gaggcgguga cccgcgccaa ccuggggcag     3960

ccgaucaucg acaaggacua caagacgacc gagggcuggc aguuccuguu cagcaugaag     4020

cagaacgagu acuucgucuu ccccaacgag aagaccgggu ucaaccccaa ggagaucgac     4080

cuccuggacg uggagaacua cggccugauc uccccgaacc ucuuccgggu gcagaaguuc     4140

agccugaaga acuacgucuu ccgccaccac cuggagacca cgaucaagga caccuccagc     4200

auccuccggg ggaucaccug gaucgacuuc cgcuccagca agggccugga caccaucgug     4260

aagguccggg ugaaccacau cggccagauc guguccgucg gggaguacug a              4311


<210>  2199
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2199
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga acaaggacga gcgguccagc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagcugac gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggacccuca agcgcgggau gcggcgcaac cugcagcggu acaagcugcg ccgggagacg      240

cucaccgagg ugcugaagga gcacaagcug aucaccgagg acaccauccu cagcgagaac      300

ggcaaccgca cgaccuucga gaccuaccgg cugcgcgcca aggccgugac cgaggagauc      360

ucccuggagg aguucgcccg gguccuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagggggu ggaggagggc acgcugaucg acgggaugga caucgcgcgc      480

gagcucuaca acaacaaccu gaccccgggc gagcugugcc uccagcugcu ggacgccggg      540

aagaaguucc uccccgacuu cuaccggagc gaccugcaga acgagcugga ccgcaucugg      600

gagaagcaga aggaguacua ccccgagauc cucaccgacg ugcugaagga ggagcugcgg      660

ggcaagaagc gcgacgccgu cugggccauc ugcgcgaagu acuucgugug gaaggagaac      720

uacaccgagu ggaacaagga gaagggcaag acggagcagc aggagcggga gcacaagcuc      780

gaggggaucu acuccaagcg caagcgcgac gaggccaagc gggagaaccu gcaguggcgc      840

gugaacggcc ugaaggagaa gcucagccug gagcagcugg ucaucguguu ccaggagaug      900

aacacccaga ucaacaacuc cagcggguac cucggcgcca ucuccgaccg gagcaaggag      960

cuguacuuca acaagcagac cgugggccag uaccagaugg agaugcugga caagaacccc     1020

aacgccuccc uccgcaacau ggucuucuac cggcaggacu accuggacga guucaacaug     1080

cugugggaga agcaggccgu guaccacaag gagcucaccg aggagcugaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cucaagagcc agaaggggcu gaucggcuuc     1200

ugcgaguucg agucccggca gaucgaggug gacaucgacg ggaagaagaa gaucaagacg     1260

gucggcaacc gcgugaucag ccgguccagc ccgcuguucc aggaguucaa gaucuggcag     1320

auccugaaca acaucgaggu gaccgucgug gggaagaagc gcaagcggcg caagcucaag     1380

gagaacuaca gcgcgcuguu cgaggagcug aacgacgccg agcagcugga gcucaacggc     1440

ucccggcgcc ugugccagga ggagaaggag cugcucgccc aggagcuguu cauccgggac     1500

aagaugacca agagcgaggu gcugaagcuc cuguucgaca acccccagga gcuggaccuc     1560

aacuucaaga ccaucgacgg caacaagacg ggguacgccc uguuccaggc cuacuccaag     1620

augaucgaga ugagcggcca cgagcccguc gacuucaaga agcccgugga gaaggucgug     1680

gaguacauca aggcgguguu cgaccugcuc aacuggaaca ccgacauccu gggguucaac     1740

uccaacgagg agcuggacaa ccagccguac uacaagcucu ggcaccuccu guacagcuuc     1800

gagggcgaca acacccccac cgggaacggc cgccugaucc agaagaugac ggagcuguac     1860

ggcuucgaga aggaguacgc caccauccug gccaacgucu ccuuccagga cgacuacggg     1920

agccucuccg ccaaggccau ccacaagauc cugccccacc ugaaggaggg caaccgguac     1980

gacguggcgu gcguguacgc cggguaccgc cacagcgagu ccagccucac ccgcgaggag     2040

aucgccaaca agguccugaa ggaccggcug augcuccugc ccaagaacuc ccugcacaac     2100

ccgguggugg agaagauccu caaccagaug gucaacguga ucaacgugau caucgacauc     2160

uacggcaagc ccgacgagau ccgcgucgag cuggcccggg agcugaagaa gaacgccaag     2220

gagcgcgagg agcucaccaa gagcaucgcg cagacgacca aggcccacga ggaguacaag     2280

acccugcugc agaccgaguu cgggcucacg aacguguccc ggaccgacau ccugcgcuac     2340

aagcuguaca aggagcucga gagcugcggc uacaagaccc uguacuccaa caccuacauc     2400

agccgggaga agcuguucuc caaggaguuc gacaucgagc acaucauccc ccaggcccgc     2460

cucuucgacg acagcuucag caacaagacc cuggaggccc gguccgugaa caucgagaag     2520

ggcaacaaga cggcguacga cuucgucaag gagaaguucg gggagagcgg cgccgacaac     2580

ucccuggagc acuaccucaa caacaucgag gaccuguuca agagcgggaa gaucuccaag     2640

accaaguaca acaagcugaa gauggccgag caggacaucc ccgacggcuu caucgagcgc     2700

gaccuccgga acacccagua caucgccaag aaggcccuga gcaugcugaa cgagaucucc     2760

caccgcgugg uggcgaccag cggcuccguc acggacaagc uccgggagga cuggcagcug     2820

aucgacguga ugaaggagcu gaacugggag aaguacaagg cccucgggcu gguggaguac     2880

uucgaggacc gcgacggccg gcagaucggg cgcaucaagg acuggaccaa gcggaacgac     2940

caccgccacc acgccaugga cgcccugacc gucgccuuca ccaaggacgu guucauccag     3000

uacuucaaca acaagaacgc gagccucgac ccgaacgcca acgagcacgc caucaagaac     3060

aaguacuucc agaacggccg ggccaucgcc cccaugcccc ugcgcgaguu ccgggcggag     3120

gccaagaagc accuggagaa cacgcucauc uccaucaagg ccaagaacaa ggugaucacc     3180

gggaacauca acaagacccg caagaagggc ggcgucaaca agaacaugca gcagacgccc     3240

cgcgggcagc ugcaccugga gaccaucuac ggcagcggga agcaguaccu caccaaggag     3300

gagaagguga acgccuccuu cgacaugcgg aagaucggca ccgugagcaa guccgcguac     3360

cgcgacgccc ugcugaagcg gcucuacgag aacgacaacg acccgaagaa ggccuucgcc     3420

gggaagaaca gccuggacaa gcagcccauc uggcuggaca aggagcagau gcgcaagguc     3480

cccgagaagg ugaagaucgu gacccucgag gccaucuaca cgauccggaa ggagaucucc     3540

cccgaccuga aggucgacaa ggugaucgac gugggcgucc gcaagauccu gaucgaccgg     3600

cucaacgagu acggcaacga cgcgaagaag gccuucagca accuggacaa gaacccgauc     3660

uggcugaaca aggagaaggg gaucagcauc aagcgcguga ccaucuccgg caucagcaac     3720

gcccaguccc uccacgugaa gaaggacaag gacgggaagc ccauccugga cgagaacggc     3780

cggaacaucc ccgucgacuu cgugaacacc gggaacaacc accacguggc cgucuacuac     3840

cgccccguga ucgacaagcg gggccagcug guggucgacg aggccggcaa cccgaaguac     3900

gagcucgagg aggugguggu cagcuucuuc gaggcgguga cccgcgccaa ccuggggcug     3960

cccaucaucg acaaggacua caagacgacc gagggcuggc aguuccucuu cuccaugaag     4020

cagaacgagu acuucguguu ccccaacgag aagaccgggu ucaacccgaa ggagaucgac     4080

cugcuggacg ucgagaacua cggccucauc agccccaacc uguuccgggu gcagaaguuc     4140

ucccugaaga acuacguguu ccgccaccac cucgagacca cgaucaagga caccagcucc     4200

auccugcggg ggaucaccug gaucgacuuc cgcagcucca agggccugga caccaucguc     4260

aaggugcggg ucaaccacau cggccagauc gugagcgugg gggaguacug a              4311


<210>  2200
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2200
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga acaaggacga gcgguccagc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagcugac gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggacccuca agcgcgggau gcggcgcaac cugcagcggu acaagcugcg ccgggagacg      240

cucaccgagg ugcugaagga gcacaagcug aucaccgagg acaccauccu cagcgagaac      300

ggcaaccgca cgaccuucga gaccuaccgg cugcgcgcca aggccgugac cgaggagauc      360

ucccuggagg aguucgcccg gguccuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagggggu ggaggagggc acgcugaucg acgggaugga caucgcgcgc      480

gagcucuaca acaacaaccu gaccccgggc gagcugugcc uccagcugcu ggacgccggg      540

aagaaguucc uccccgacuu cuaccggagc gaccugcaga acgagcugga ccgcaucugg      600

gagaagcaga aggaguacua ccccgagauc cucaccgacg ugcugaagga ggagcugcgg      660

ggcaagaagc gcgacgccgu cugggccauc ugcgcgaagu acuucgugug gaaggagaac      720

uacaccgagu ggaacaagga gaagggcaag acggagcagc aggagcggga gcacaagcuc      780

gaggggaucu acuccaagcg caagcgcgac gaggccaagc gggagaaccu gcaguggcgc      840

gugaacggcc ugaaggagaa gcucagccug gagcagcugg ucaucguguu ccaggagaug      900

aacacccaga ucaacaacuc cagcggguac cucggcgcca ucuccgaccg gagcaaggag      960

cuguacuuca acaagcagac cgugggccag uaccagaugg agaugcugga caagaacccc     1020

aacgccuccc uccgcaacau ggucuucuac cggcaggacu accuggacga guucaacaug     1080

cugugggaga agcaggccgu guaccacaag gagcucaccg aggagcugaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cucaagagcc agaaggggcu gaucggcuuc     1200

ugcgaguucg agucccggca gaucgaggug gacaucgacg ggaagaagaa gaucaagacg     1260

gucggcaacc gcgugaucag ccgguccagc ccgcuguucc aggaguucaa gaucuggcag     1320

auccugaaca acaucgaggu gaccgucgug gggaagaagc gcaagcggcg caagcucaag     1380

gagaacuaca gcgcgcuguu cgaggagcug aacgacgccg agcagcugga gcucaacggc     1440

ucccggcgcc ugugccagga ggagaaggag cugcucgccc aggagcuguu cauccgggac     1500

aagaugacca agagcgaggu gcugaagcuc cuguucgaca acccccagga gcuggaccuc     1560

aacuucaaga ccaucgacgg caacaagacg ggguacgccc uguuccaggc cuacuccaag     1620

augaucgaga ugagcggcca cgagcccguc gacuucaaga agcccgugga gaaggucgug     1680

gaguacauca aggcgguguu cgaccugcuc aacuggaaca ccgacauccu gggguucaac     1740

uccaacgagg agcuggacaa ccagagcuac uacaagcucu ggcaccuccu guacuccuuc     1800

gagggcgaca acaccccgac cgggaacggc cgccugaucc agaagaugac ggagcuguac     1860

ggcuucgaga aggaguacgc caccauccug gccaacguca gcuuccagga cgacuacggg     1920

ucccucagcg ccaaggccau ccacaagauc cugccccacc ugaaggaggg caaccgguac     1980

gacguggcgu gcguguacgc cggguaccgc cacuccgaga gcucccucac ccgcgaggag     2040

aucgccaaca agguccugaa ggaccggcug augcuccugc ccaagaacag ccugcacaac     2100

cccguggugg agaagauccu caaccagaug gucaacguga ucaacgugau caucgacauc     2160

uacggcaagc cggacgagau ccgcgucgag cuggcccggg agcugaagaa gaacgccaag     2220

gagcgcgagg agcucaccaa guccaucgcg cagacgacca aggcccacga ggaguacaag     2280

acccugcugc agaccgaguu cgggcucacg aacgugagcc ggaccgacau ccugcgcuac     2340

aagcuguaca aggagcucga guccugcggc uacaagaccc uguacagcaa caccuacauc     2400

ucccgggaga agcuguucag caaggaguuc gacaucgagc acaucauccc ccaggcccgc     2460

cucuucgacg acagcuucuc caacaagacc cuggaggccc ggagcgugaa caucgagaag     2520

ggcaacaaga cggcguacga cuucgucaag gagaaguucg gggaguccgg cgccgacaac     2580

agccuggagc acuaccucaa caacaucgag gaccuguuca aguccgggaa gaucagcaag     2640

accaaguaca acaagcugaa gauggccgag caggacaucc ccgacggcuu caucgagcgc     2700

gaccuccgga acacccagua caucgccaag aaggcccugu ccaugcugaa cgagaucagc     2760

caccgcgugg uggcgaccuc cggcagcguc acggacaagc uccgggagga cuggcagcug     2820

aucgacguga ugaaggagcu gaacugggag aaguacaagg cccucgggcu gguggaguac     2880

uucgaggacc gcgacggccg gcagaucggg cgcaucaagg acuggaccaa gcggaacgac     2940

caccgccacc acgccaugga cgcccugacc gucgccuuca ccaaggacgu guucauccag     3000

uacuucaaca acaagaacgc gucccucgac cccaacgcca acgagcacgc caucaagaac     3060

aaguacuucc agaacggccg ggccaucgcc ccgaugcccc ugcgcgaguu ccgggcggag     3120

gccaagaagc accuggagaa cacgcucauc agcaucaagg ccaagaacaa ggugaucacc     3180

gggaacauca acaagacccg caagaagggc ggcgucaaca agaacaugca gcagacgccc     3240

cgcgggcagc ugcaccugga gaccaucuac ggcuccggga agcaguaccu caccaaggag     3300

gagaagguga acgccagcuu cgacaugcgg aagaucggca ccguguccaa gcuggcguac     3360

cgcgacgccc ugcucaagcg gcuguacgag aacgacaacg accccaagaa ggccuucgcc     3420

gggaagaaca gccuggacaa gcagccgauc uggcucgaca aggagcagau gcgcaagguc     3480

cccgagaagg ugaagaucgu gacccuggag gccaucuaca cgauccggaa ggagaucucc     3540

cccgaccuga aggucgacaa ggugaucgac gugggcgucc gcaagauccu caucgaccgg     3600

cugaacgagu acggcaacga cgcgaagaag gccuucagca accuggacga gaaccccauc     3660

uggcucaaca aggagaaggg gaucagcauc aagcgcguga ccaucuccgg caucagcaac     3720

gcccaguccc ugcacgugaa gaaggacaag gacgggaagc cgauccugga cgagaacggc     3780

cggaacaucc ccgucgacuu cgugaacacc gggaacaacc accacguggc cgucuacuac     3840

cgccccguga ucgacaagcg gggccagcuc guggucgacg aggccggcaa ccccaaguac     3900

gagcuggagg aggugguggu cagcuucuuc gaggcgguga cccgcgccaa ccuggggcag     3960

ccgaucaucg acaaggacua caagacgacc gagggcuggc aguuccucuu cuccaugaag     4020

cagaacgagu acuucguguu ccccaacgag aagaccgggu ucaaccccaa ggagaucgac     4080

cugcuggacg ucgagaacua cggccucauc agcccgaacc uguuccgggu gcagaaguuc     4140

ucccugaaga acuacguguu ccgccaccac cucgagacca cgaucaagga caccagcucc     4200

auccugcggg ggaucaccug gaucgacuuc cgcagcucca agggccugga caccaucguc     4260

aaggugcggg ucaaccacau cggccagauc gugagcgugg gggaguacug a              4311


<210>  2201
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2201
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga acaaggacga gcgguccagc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagcugac gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggacccuca agcgcgggau gcggcgcaac cugcagcggu acaagcugcg ccgggagacg      240

cucaccgagg ugcugaagga gcacaagcug aucaccgagg acaccauccu cagcgagaac      300

ggcaaccgca cgaccuucga gaccuaccgg cugcgcgcca aggccgugac cgaggagauc      360

ucccuggagg aguucgcccg gguccuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagggggu ggaggagggc acgcugaucg acgggaugga caucgcgcgc      480

gagcucuaca acaacaaccu gaccccgggc gagcugugcc uccagcugcu ggacgccggg      540

aagaaguucc uccccgacuu cuaccggagc gaccugcaga acgagcugga ccgcaucugg      600

gagaagcaga aggaguacua ccccgagauc cucaccgacg ugcugaagga ggagcugcgg      660

ggcaagaagc gcgacgccgu cugggccauc ugcgcgaagu acuucgugug gaaggagacc      720

uacacggagu ggaacaagga gaagggcaag accgagcagc aggagcggga gcacaagcuc      780

gaggggaucu acuccaagcg caagcgcgac gaggccaagc gggagaaccu gcaguggcgc      840

gugaacggcc ugaaggagaa gcucagccug gagcagcugg ucaucguguu ccaggagaug      900

aacacccaga ucaacaacuc cagcggguac cucggcgcca ucuccgaccg gagcaaggag      960

cuguacuuca acaagcagac cgugggccag uaccagaugg agaugcugga caagaacccc     1020

aacgccuccc uccgcaacau ggucuucuac cggcaggacu accuggacga guucaacaug     1080

cugugggaga agcaggccgu guaccacaag gagcucacgg aggagcugaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cucaagagcc agaaggggcu caucggcuuc     1200

ugcgaguucg agucccggca gaucgaggug gacaucgacg ggaagaagaa gaucaagacc     1260

gucggcaacc gcgugaucag ccgguccagc ccgcuguucc aggaguucaa gaucuggcag     1320

auccugaaca acaucgaggu gaccgucgug gggaagaagc gcaagcggcg caagcucaag     1380

gagaacuaca gcgcgaucuu cgaggagcug aacgacgccg agcagcugga gcugaacggc     1440

ucccggcgcc ucugccagga ggagaaggag cugcuggccc aggagcuguu cgugcgggac     1500

aagaugacca agagcgaggu ccugaagcuc cuguucgaca acccccagga gcuggaccug     1560

aacuucaaga cgaucgacgg caacaagacc ggguacgccc ucuuccaggc cuacuccaag     1620

augaucgaga ugagcggcca cgagcccgug gacuucaaga agcccgucga gaagguggug     1680

gaguacauca aggcggucuu cgaccugcug aacuggaaca ccgacauccu cggguucaac     1740

uccaacgagg agcuggacaa ccagagcuac uacaagcugu ggcaccuccu guacuccuuc     1800

gagggcgaca acaccccgac ggggaacggc cgccugaucc agaagaugac cgagcucuac     1860

ggcuucgaga aggaguacgc caccauccug gccaacguga gcuuccagga cgacuacggg     1920

ucccugagcg ccaaggccau ccacaagauc cucccccacc ugaaggaggg caaccgguac     1980

gacguggcgu gcgucuacgc cggguaccgc cacuccgaga gcucccugac ccgcgaggag     2040

aucgccaaca aggugcucaa ggaccggcug augcugcucc ccaagaacag ccugcacaac     2100

cccguggucg agaagauccu gaaccagaug gugaacguga ucaacgucau caucgacauc     2160

uacggcaagc cggacgagau ccgcguggag cucgcccggg agcugaagaa gaacgccaag     2220

gagcgcgagg agcugacgaa guccaucgcg cagaccacca aggccaacga ggaguacaag     2280

acccuccugc agacggaguu cgggcugacc aacgugagcc ggaccgacau ccuccgcuac     2340

aagcuguaca aggagcugga guccugcggc uacaagaccc ucuacagcaa caccuacauc     2400

ucccgggaga agcuguucag caaggaguuc gacaucgagc acaucauccc ccaggcccgc     2460

cuguucgacg acagcuucuc caacaagacg cucgaggccc ggagcgucaa caucgagaag     2520

ggcaacaaga ccgcguacga cuucgugaag gagaaguucg gggaguccgg cgccgacaac     2580

agccuggagc acuaccugaa caacaucgag gaccucuuca aguccgggaa gaucagcaag     2640

accaaguaca acaagcugaa gauggccaag caggacaucc ccgacggcuu caucgagcgc     2700

gaccugcgga acacccagua caucgccaag aaggcccucu ccaugcugaa cgagaucagc     2760

caccgcgugg ucgcgacguc cggcagcgug accgacaagc ugcgggagga cuggcagcuc     2820

aucgacguga ugaaggagcu gaacugggag aaguacaagg cccuggggcu cgucgaguac     2880

uucgaggacc gcgacggccg gcagaucggg cgcaucaagg acuggaccaa gcggaacgac     2940

caccgccacc acgccaugga cgcccugacc guggccuuca cgaaggacgu guucauccag     3000

uacuucaaca acaagaacgc gucccuggac cccaacgcca acgagcacgc caucaagaac     3060

aaguacuucc agaacggccg ggccaucgcc ccgaugcccc uccgcgaguu ccgggcggag     3120

gccaagaagc accuggagaa cacccugauc agcaucaagg ccaagaacaa ggucaucacc     3180

gggaacauca acaagacccg caagaagggc ggcgugaaca agaacaugca gcagacgccc     3240

cgcgggcagc uccaccugga gaccaucuac ggcuccggga agcaguaccu gaccaaggag     3300

gagcggguga acgccagcuu cgacaugcgc aagaucgaga ccgucuccaa gagcgcguac     3360

cgggacgccc uccugaagcg ccuguacgag aacgacaacg accccaagaa ggccuucgcc     3420

ggcaagaacu cccucgacaa gcagccgauc uggcuggaca aggagcagaa gcggaaggug     3480

cccgagaagg ugaagaucgu cacgcuggag gccaucuaca ccauccgcaa ggagaucagc     3540

cccgaccuca agguggacaa ggugaucgac gucggggugc ggaagauccu gaucgaccgc     3600

cugaacgagu acggcaacga cgcgaagaag gccuucagca accucgacga gaaccccauc     3660

uggcugaaca aggagaaggg caucuccauc aagcggguga ccaucagcgg gaucuccaac     3720

gcccagagcc ugcacgucaa gaaggacaag gacggcaagc cgauccucga cgagaacggg     3780

cgcaacaucc ccguggacuu cgugaacacc ggcaacaacc accacgucgc cguguacuac     3840

cggcccguga ucgacaagcg cgggcagcug gucguggacg aggccggcaa ccccaaguac     3900

gagcuggagg agguggucgu guccuucuuc gaggcgguga cgcgggccaa ccucggccag     3960

ccgaucaucg acaaggacua caagaccacc gagggguggc aguuccuguu cagcaugaag     4020

cagaacgagu acuucgucuu ccccaacgag aagaccggcu ucaaccccaa ggagaucgac     4080

cugcucgacg uggagaacua cgggcugauc uccccgaacc uguuccgcgu gcagaaguuc     4140

agccucaaga acuacgucuu ccggcaccac cuggagacga ccaucaagga caccuccagc     4200

auccugcgcg gcaucaccug gaucgacuuc cgguccagca aggggcucga cacgaucgug     4260

aagguccgcg ugaaccacau cggccagauc guguccgucg gcgaguacug a              4311


<210>  2202
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2202
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga acaaggacga gcgguccagc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagcugac gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggacccuca agcgcgggau gcggcgcaac cugcagcggu acaagcugcg ccgggagacg      240

cucaccgagg ugcugaagga gcacaagcug aucaccgagg acaccauccu cagcgagaac      300

ggcaaccgca cgaccuucga gaccuaccgg cugcgcgcca aggccgugac cgaggagauc      360

ucccuggagg aguucgcccg gguccuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagggggu ggaggagggc acgcugaucg acgggaugga caucgcgcgc      480

gagcucuaca acaacaaccu gaccccgggc gagcugugcc uccagcugcu ggacgccggg      540

aagaaguucc uccccgacuu cuaccggagc gaccugcaga acgagcugga ccgcaucugg      600

gagaagcaga aggaguacua ccccgagauc cucaccgacg ugcugaagga ggagcugcgg      660

ggcaagaagc gcgacgccgu cugggccauc ugcgcgaagu acuucgugug gaaggagacc      720

uacacggagu ggaacaagga gaagggcaag accgagcagc aggagaagga gcacaagcuc      780

gaggggaucu acuccaagcg gaagcgcgac gaggccaagc gcgagaaccu gcaguggcgg      840

gugaacggcc ugaaggagaa gcucagccug gagcagcugg ucaucguguu ccaggagaug      900

aacacccaga ucaacaacuc cagcggguac cucggcgcca ucuccgaccg cagcaaggag      960

cuguacuuca acaagcagac cgugggccag uaccagaugg agaugcugga caagaacccc     1020

aacgccuccc uccggaacau ggucuucuac cgccaggacu accuggacga guucaacaug     1080

cugugggaga agcaggccgu guaccacaag gagcucacgg aggagcugaa gaaggagauc     1140

cgggacauca ucaucuucua ccagcgccgg cucaagagcc agaaggggcu caucggcuuc     1200

ugcgaguucg agucccgcca gaucgaggug gacaucgacg ggaagaagaa gaucaagacc     1260

gucggcaacc gggugaucag ccgcuccagc ccgcuguucc aggaguucaa gaucuggcag     1320

auccugaaca acaucgaggu gaccgucgug gggaagaagc ggaagcgccg gaagcucaag     1380

gagaacuaca gcgcgcuguu cgaggagcug aacgacgccg agcagcugga gcucaacggc     1440

ucccgccggc ugugccagga ggagaaggag cugcucgccc aggagcuguu cauccgcgac     1500

aagaugacca agagcgaggu gcugaagcuc cuguucgaca acccccagga gcuggaccuc     1560

aacuucaaga cgaucgacgg caacaagacc ggguacgccc uguuccaggc cuacuccaag     1620

augaucgaga ugagcggcca cgagcccguc gacuucaaga agcccgugga gaaggucgug     1680

gaguacauca aggcgguguu cgaccugcuc aacuggaaca ccgacauccu gggguucaac     1740

uccaacgagg agcuggacaa ccagagcuac uacaagcucu ggcaccuccu guacuccuuc     1800

gagggcgaca acaccccgac ggggaacggc cggcugaucc agaagaugac cgagcuguac     1860

ggcuucgaga aggaguacgc caccauccug gccaacguca gcuuccagga cgacuacggg     1920

ucccucagcg ccaaggccau ccacaagauc cugccccacc ugaaggaggg caaccgcuac     1980

gacguggcgu gcguguacgc cggguaccgg cacuccgaga gcucccucac ccgcgaggag     2040

aucgccaaca agguccugaa ggaccgccug augcuccugc ccaagaacag ccugcacaac     2100

cccguggugg agaagauccu caaccagaug gucaacguga ucaacgugau caucgacauc     2160

uacggcaagc cggacgagau ccgggucgag cuggcccgcg agcugaagaa gaacgccaag     2220

gagcgggagg agcucacgaa guccaucgcg cagaccacca aggcccacga ggaguacaag     2280

acccugcugc agacggaguu cgggcucacc aacgugagcc gcaccgacau ccugcgguac     2340

aagcuguaca aggagcucga guccugcggc uacaagaccc uguacagcaa caccuacauc     2400

ucccgcgaga agcuguucag caaggaguuc gacaucgagc acaucauccc ccaggcccgg     2460

cuguucgacg acagcuucuc caacaagacg cuggaggccc gcagcgugaa caucgagaag     2520

ggcaacaaga ccgcguacga cuucgucaag gagaaguucg gggaguccgg cgccgacaac     2580

agccuggagc acuaccucaa caacaucgag gaccuguuca aguccgggaa gaucagcaag     2640

accaaguaca acaagcugaa gauggccgag caggacaucc ccgacggcuu caucgagcgg     2700

gaccuccgca acacccagua caucgccaag aaggcccugu ccaugcugaa cgagaucagc     2760

caccgggugg uggcgacguc cggcagcguc accgacaagc uccgcgagga cuggcagcug     2820

aucgacguga ugaaggagcu gaacugggag aaguacaagg cccucgggcu gguggaguac     2880

uucgaggacc gggacggccg ccagaucggg cggaucaagg acuggaccaa gcgcaacgac     2940

caccggcacc acgccaugga cgcccugacc gucgccuuca cgaaggacgu guucauccag     3000

uacuucaaca acaagaacgc gucccucgac cccaacgcca acgagcacgc caucaagaac     3060

aaguacuucc agaacggccg cgccaucgcc ccgaugcccc ugcgggaguu ccgcgcggag     3120

gccaagaagc accuggagaa cacccucauc agcaucaagg ccaagaacaa ggugaucacc     3180

gggaacauca acaagacccg gaagaagggc ggcgucaaca agaacaugca gcagacgccc     3240

cgcgggcagc ugcaccugga gaccaucuac ggcuccggga agcaguaccu caccaaggag     3300

gagaagguga acgccagcuu cgacaugcgc aagaucggca ccguguccaa gcuggcguac     3360

cgggacgccc ugcucaagcg ccuguacgag aacgacaacg accccaagaa ggccuucgcc     3420

gggaagaaca gccuggacaa gcagccgauc uggcucgaca aggagcagau gcggaagguc     3480

cccgagaagg ugaagaucgu gacgcuggag gccaucuaca ccauccgcaa ggagaucucc     3540

cccgaccuga aggucgacaa ggugaucgac gugggcgucc ggaagauccu caucgaccgc     3600

cugaacgagu acggcaacga cgcgaagaag gccuucagca accuggacga gaaccccauc     3660

uggcucaaca aggagaaggg gaucagcauc aagcggguga ccaucuccgg caucagcaac     3720

gcccaguccc ugcacgugaa gaaggacaag gacgggaagc cgauccugga cgagaacggc     3780

cgcaacaucc ccgucgacuu cgugaacacc gggaacaacc accacguggc cgucuacuac     3840

cggcccguga ucgacaagcg cggccagcuc guggucgacg aggccggcaa ccccaaguac     3900

gagcuggagg aggugguggu cagcuucuuc gaggcgguga cgcgggccaa ccuggggcag     3960

ccgaucaucg acaaggacua caagaccacc gagggcuggc aguuccucuu cuccaugaag     4020

cagaacgagu acuucguguu ccccaacgag aagaccgggu ucaaccccaa ggagaucgac     4080

cugcuggacg ucgagaacua cggccucauc agcccgaacc uguuccgcgu gcagaaguuc     4140

ucccugaaga acuacguguu ccggcaccac cucgagacga ccaucaagga caccagcucc     4200

auccugcgcg ggaucaccug gaucgacuuc cggagcucca agggccugga cacgaucguc     4260

aaggugcgcg ucaaccacau cggccagauc gugagcgugg gggaguacug a              4311


<210>  2203
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2203
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga acaaggacga gcgguccagc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagcugac gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggacccuca agcgcgggau gcggcgcaac cugcagcggu acaagcugcg ccgggagacg      240

cucaccgagg ugcugaagga gcacaagcug aucaccgagg acaccauccu cagcgagaac      300

ggcaaccgca cgaccuucga gaccuaccgg cugcgcgcca aggccgugac cgaggagauc      360

ucccuggagg aguucgcccg gguccuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagggggu ggaggagggc acgcugaucg acgggaugga caucgcgcgc      480

gagcucuaca acaacaaccu gaccccgggc gagcugugcc uccagcugcu ggacgccggg      540

aagaaguucc uccccgacuu cuaccggagc gaccugcaga acgagcugga ccgcaucugg      600

gagaagcaga aggaguacua ccccgagauc cucaccgacg ugcugaagga ggagcugcgg      660

ggcaagaagc gcgacgccgu cugggccauc ugcgcgaagu acuucgugug gaaggagaac      720

uacaccgagu ggaacaagga gaagggcaag acggagcagc aggagcggga gcacaagcuc      780

gaggggaucu acuccaagcg caagcgcgac gaggccaagc gggagaaccu gcaguggcgc      840

gugaacggcc ugaaggagaa gcucagccug gagcagcugg ucaucguguu ccaggagaug      900

aacacccaga ucaacaacuc cagcggguac cucggcgcca ucuccgaccg gagcaaggag      960

cuguacuuca acaagcagac cgugggccag uaccagaugg agaugcugga caagaacccc     1020

aacgccuccc uccgcaacau ggucuucuac cggcaggacu accuggacga guucaacaug     1080

cugugggaga agcaggccgu guaccacaag gagcucaccg aggagcugaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cucaagagcc agaaggggcu gaucggcuuc     1200

ugcgaguucg agucccggca gaucgaggug gacaucgacg ggaagaagaa gaucaagacg     1260

gucggcaacc gcgugaucag ccgguccagc ccgcuguucc aggaguucaa gaucuggcag     1320

auccugaaca acaucgaggu gaccgucgug gggaagaagc gcaagcggcg caagcucaag     1380

gagaacuaca gcgcgcuguu cgaggagcug aacgacgccg agcagcugga gcucaacggc     1440

ucccggcgcc ugugccagga ggagaaggag cugcucgccc aggagcuguu cauccgggac     1500

aagaugacca agagcgaggu gcugaagcuc cuguucgaca acccccagga gcuggaccuc     1560

aacuucaaga ccaucgacgg caacaagacg ggguacgccc uguuccaggc cuacuccaag     1620

augaucgaga ugagcggcca cgagcccguc gacuucaaga agcccgugga gaaggucgug     1680

gaguacauca aggcgguguu cgaccugcuc aacuggaaca ccgacauccu gggguucaac     1740

uccaacgagg agcuggacaa ccagccguac uacaagcucu ggcaccuccu guacagcuuc     1800

gagggcgaca acacccccac cgggaacggc cgccugaucc agaagaugac ggagcuguac     1860

ggcuucgaga aggaguacgc caccauccug gccaacgucu ccuuccagga cgacuacggg     1920

agccucuccg ccaaggccau ccacaagauc cugccccacc ugaaggaggg caaccgguac     1980

gacguggcgu gcguguacgc cggguaccgc cacagcgagu ccagccucac ccgcgaggag     2040

aucgccaaca agguccugaa ggaccggcug augcuccugc ccaagaacuc ccugcacaac     2100

ccgguggugg agaagauccu caaccagaug gucaacguga ucaacgugau caucgacauc     2160

uacggcaagc ccgacgagau ccgcgucgag cuggcccggg agcugaagaa gaacgccaag     2220

gagcgcgagg agcucaccaa gagcaucgcg cagacgacca aggcccacga ggaguacaag     2280

acccugcugc agaccgaguu cgggcucacg aacguguccc ggaccgacau ccugcgcuac     2340

aagcuguaca aggagcucga gagcugcggc uacaagaccc uguacuccaa caccuacauc     2400

agccgggaga agcuguucuc caaggaguuc gacaucgagc acaucauccc ccaggcccgc     2460

cucuucgacg acagcuucag caacaagacc cuggaggccc gguccgugaa caucgagaag     2520

ggcaacaaga cggcguacga cuucgucaag gagaaguucg gggagagcgg cgccgacaac     2580

ucccuggagc acuaccucaa caacaucgag gaccuguuca agagcgggaa gaucuccaag     2640

accaaguaca acaagcugaa gauggccgag caggacaucc ccgacggcuu caucgagcgc     2700

gaccuccgga acacccagua caucgccaag aaggcccuga gcaugcugaa cgagaucucc     2760

caccgcgugg uggcgaccag cggcuccguc acggacaagc uccgggagga cuggcagcug     2820

aucgacguga ugaaggagcu gaacugggag aaguacaagg cccucgggcu gguggaguac     2880

uucgaggacc gcgacggccg gcagaucggg cgcaucaagg acuggaccaa gcggaacgac     2940

caccgccacc acgccaugga cgcccugacc gucgccuuca ccaaggacgu guucauccag     3000

uacuucaaca acaagaacgc gagccucgac ccgaacgcca acgagcacgc caucaagaac     3060

aaguacuucc agaacggccg ggccaucgcc cccaugcccc ugcgcgaguu ccgggcggag     3120

gccaagaagc accuggagaa cacgcucauc uccaucaagg ccaagaacaa ggugaucacc     3180

gggaacauca acaagacccg caagaagggc ggcgucaaca agaacaugca gcagacgccc     3240

cgcgggcagc ugcaccugga gaccaucuac ggcagcggga agcaguaccu caccaaggag     3300

gagaagguga acgccuccuu cgacaugcgg aagaucggca ccgugagcaa guccgcguac     3360

cgcgacgccc ugcugaagcg gcucuacgag aacgacaacg acccgaagaa ggccuucgcc     3420

gggaagaaca gccuggacaa gcagcccauc uggcuggaca aggagcagau gcgcaagguc     3480

cccgagaagg ugaagaucgu gacccucgag gccaucuaca cgauccggaa ggagaucucc     3540

agcgaccuga aggucgacaa ggugaucgac gugggcgucc gcaagauccu gaucgaccgg     3600

cucaacgagu acggcaacga cgcgaagaag gccuucagca accuggacaa gaaccccauc     3660

uggcugaaca aggagaaggg gaucuccauc aagcgcguga ccaucagcgg caucuccaac     3720

gcccagagcc uccacgugaa gaaggacaag gacgggaagc cgauccugga cgagaacggc     3780

cggaacaucc ccgucgacuu cgugaacacc gggaacaacc accacguggc cgucuacuac     3840

cgccccguga ucgacaagcg gggccagcug guggucgacg aggccggcaa ccccaaguac     3900

gagcucgagg aggugguggu cuccuucuuc gaggcgguga cccgcgccaa ccuggggcug     3960

ccgaucaucg acaaggacua caagacgacc gagggcuggc aguuccucuu cagcaugaag     4020

cagaacgagu acuucguguu ccccaacgag aagaccgggu ucaaccccaa ggagaucgac     4080

cugcuggacg ucgagaacua cggccucauc uccccgaacc uguuccgggu gcagaaguuc     4140

agccugaaga acuacguguu ccgccaccac cucgagacca cgaucaagga caccuccagc     4200

auccugcggg ggaucaccug gaucgacuuc cgcuccagca agggccugga caccaucguc     4260

aaggugcggg ucaaccacau cggccagauc guguccgugg gggaguacug a              4311


<210>  2204
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2204
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga acaaggacga gcgguccagc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagcugac gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggacccuca agcgcgggau gcggcgcaac cugcagcggu acaagcugcg ccgggagacg      240

cucaccgagg ugcugaagga gcacaagcug aucaccgagg acaccauccu cagcgagaac      300

ggcaaccgca cgaccuucga gaccuaccgg cugcgcgcca aggccgugac cgaggagauc      360

ucccuggagg aguucgcccg gguccuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagggggu ggaggagggc acgcugaucg acgggaugga caucgcgcgc      480

gagcucuaca acaacaaccu gaccccgggc gagcugugcc uccagcugcu ggacgccggg      540

aagaaguucc uccccgacuu cuaccggagc gaccugcaga acgagcugga ccgcaucugg      600

gagaagcaga aggaguacua ccccgagauc cucaccgacg ugcugaagga ggagcugcgg      660

ggcaagaagc gcgacgccgu cugggccauc ugcgcgaagu acuucgugug gaaggagaac      720

uacaccgagu ggaacaagga gaagggcaag acggagcagc aggagcggga gcacaagcuc      780

gaggggaucu acuccaagcg caagcgcgac gaggccaagc gggagaaccu gcaguggcgc      840

gugaacggcc ugaaggagaa gcucagccug gagcagcugg ucaucguguu ccaggagaug      900

aacacccaga ucaacaacuc cagcggguac cucggcgcca ucuccgaccg gagcaaggag      960

cuguacuuca acaagcagac cgugggccag ugccagaugg agaugcugga caagaacccc     1020

aacgccuccc uccgcaacau ggucuucuac cggcaggacu accuggacga guucaacaug     1080

cugugggaga agcaggccgu guaccacaag gagcucaccg aggagcugaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cucaagagcc agaaggggcu gaucggcuuc     1200

ugcgaguucg agucccggca gaucgaggug gacaucgacg ggaagaagaa gaucaagacg     1260

gucggcaacc gcgugaucag ccgguccagc ccgcuguucc aggaguucaa gaucuggcag     1320

auccugaaca acaucgaggu gaccgucgug gggaagaagc gcaagcggcg caagcucaag     1380

gagaacuaca gcgcgcuguu cgaggagcug aacgacgccg agcagcugga gcucaacggc     1440

ucccggcgcc ugugccagga ggagaaggag cugcucgccc aggagcuguu cauccgggac     1500

aagaugacca agagcgaggu gcugaagcuc cuguucgaca acccccagga gcuggaccuc     1560

aacuucaaga ccaucgacgg caacaagacg ggguacgccc uguuccaggc cuacuccaag     1620

augaucgaga ugagcggcca cgagcccguc gacuucaaga agcccgugga gaaggucgug     1680

gaguacauca aggcgguguu cgaccugcuc aacuggaaca ccgacauccu gggguucaac     1740

uccaacgagg agcuggacaa ccagccguac uacaagcucu ggcaccuccu guacagcuuc     1800

gagggcgaca acacccccac cgggaacggc cgccugaucc agaagaugac ggagcuguac     1860

ggcuucgaga aggaguacgc caccauccug gccaacgucu ccuuccagga cgacuacggg     1920

agccucuccg ccaaggccau ccacaagauc cugccccacc ugaaggaggg caaccgguac     1980

gacguggcgu gcguguacgc cggguaccgc cacagcgagu ccagccucac ccgcgaggag     2040

aucgccaaca agguccugaa ggaccggcug augcuccugc ccaagaacuc ccugcacaac     2100

ccgguggugg agaagauccu caaccagaug gucaacguga ucaacgugau caucgacauc     2160

uacggcaagc ccgacgagau ccgcgucgag cuggcccggg agcugaagaa gaacgccaag     2220

gagcgcgagg agcucaccaa gagcaucgcg cagacgacca aggcccacga ggaguacaag     2280

acccugcugc agaccgaguu cgggcucacg aacguguccc ggaccgacau ccugcgcuac     2340

aagcuguaca aggagcucga gagcugcggc uacaagaccc uguacuccaa caccuacauc     2400

agccgggaga agcuguucuc caaggaguuc gacaucgagc acaucauccc ccaggcccgc     2460

cucuucgacg acagcuucag caacaagacc cuggaggccc gguccgugaa caucgagaag     2520

ggcaacaaga cggcguacga cuucgucaag gagaaguucg gggagagcgg cgccgacaac     2580

ucccuggagc acuaccucaa caacaucgag gaccuguuca agagcgggaa gaucuccaag     2640

accaaguaca acaagcugaa gauggccgag caggacaucc ccgacggcuu caucgagcgc     2700

gaccuccgga acacccagua caucgccaag aaggcccuga gcaugcugaa cgagaucucc     2760

caccgcgugg uggcgaccag cggcuccguc acggacaagc uccgggagga cuggcagcug     2820

aucgacguga ugaaggagcu gaacugggag aaguacaagg cccucgggcu gguggaguac     2880

uucgaggacc gcgacggccg gcagaucggg cgcaucaagg acuggaccaa gcggaacgac     2940

caccgccacc acgccaugga cgcccugacc gucgccuuca ccaaggacgu guucauccag     3000

uacuucaaca acaagaacgc gagccucgac ccgaacgcca acgagcacgc caucaagaac     3060

aaguacuucc agaacggccg ggccaucgcc cccaugcccc ugcgcgaguu ccgggcggag     3120

gccaagaagc accuggagaa cacgcucauc uccaucaagg ccaagaacaa ggugaucacc     3180

gggaacauca acaagacccg caagaagggc ggcgucaaca agaacaugca gcagacgccc     3240

cgcgggcagc ugcaccugga gaccaucuac ggcagcggga agcaguaccu caccaaggag     3300

gagaagguga acgccuccuu cgacaugcgg aagaucggca ccgugagcaa guccgcguac     3360

cgcgacgccc ugcugaagcg gcucuacgag aacgacaacg acccgaagaa ggccuucgcc     3420

gggaagaaca gccuggacaa gcagcccauc uggcuggaca aggagcagau gcgcaagguc     3480

cccgagaagg ugaagaucgu gacccucgag gccaucuaca cgauccggaa ggagaucucc     3540

cccgaccuga aggucgacaa ggugaucgac gugggcgucc gcaagauccu gaucgaccgg     3600

cucaacgagu acggcaacga cgcgaagaag gccuucagca accuggacaa gaacccgauc     3660

uggcugaaca aggagaaggg gaucagcauc aagcgcguga ccaucuccgg caucagcaac     3720

gcccaguccc uccacgugaa gaaggacaag gacgggaagc ccauccugga cgagaacggc     3780

cggaacaucc ccgucgacuu cgugaacacc gggaacaacc accacguggc cgucuacuac     3840

cgccccguga ucgacaagcg gggccagcug guggucgacg aggccggcaa cccgaaguac     3900

gagcucgagg aggugguggu cagcuucuuc gaggcgguga cccgcgccaa ccuggggcug     3960

cccaucaucg acaaggacua caagacgacc gagggcuggc aguuccucuu cuccaugaag     4020

cagaacgagu acuucguguu ccccaacgag aagaccgggu ucaacccgaa ggagaucgac     4080

cugcuggacg ucgagaacua cggccucauc agccccaacc uguuccgggu gcagaaguuc     4140

ucccugaaga acuacguguu ccgccaccac cucgagacca cgaucaagga caccagcucc     4200

auccugcggg ggaucaccug gaucgacuuc cgcagcucca agggccugga caccaucguc     4260

aaggugcggg ucaaccacau cggccagauc gugagcgugg gggaguacug a              4311


<210>  2205
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2205
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gaggccgaga acaaggacga gcgguccagc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagcugac gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggacccuca agcgcgggau gcggcgcaac cugcagcggu acaagcugcg ccgggagacg      240

cucaccgagg ugcugaagga gcacaagcug aucaccgagg acaccauccu cagcgagaac      300

ggcaaccgca cgaccuucga gaccuaccgg cugcgcgcca aggccgugac cgaggagauc      360

ucccuggagg aguucgcccg gguccuccug augaucaaca agaagcgcgg cuacaagagc      420

ucccggaagg ccaagggggu ggaggagggc acgcugaucg acgggaugga caucgcgcgc      480

gagcucuaca acaacaaccu gaccccgggc gagcugugcc uccagcugcu ggacgccggg      540

aagaaguucc uccccgacuu cuaccggagc gaccugcaga acgagcugga ccgcaucugg      600

gagaagcaga aggaguacua ccccgagauc cucaccgacg ugcugaagga ggagcugcgg      660

ggcaagaagc gcgacgccgu cugggccauc ugcgcgaagu acuucgugug gaaggagacc      720

uacacggagu ggaacaagga gaagggcaag accgagcagc aggagcggga gcacaagcuc      780

gaggggaucu acuccaagcg caagcgcgac gaggccaagc gggagaaccu gcaguggcgc      840

gugaacggcc ugaaggagaa gcucagccug gagcagcugg ucaucguguu ccaggagaug      900

aacacccaga ucaacaacuc cagcggguac cucggcgcca ucuccgaccg gagcaaggag      960

cuguacuuca acaagcagac cgugggccag uaccagaugg agaugcugga caagaacccc     1020

aacgccuccc uccgcaacau ggucuucuac cggcaggacu accuggacga guucaacaug     1080

cugugggaga agcaggccgu guaccacaag gagcucacgg aggagcugaa gaaggagauc     1140

cgcgacauca ucaucuucua ccagcggcgc cucaagagcc agaaggggcu caucggcuuc     1200

ugcgaguucg agucccggca gaucgaggug gacaucgacg ggaagaagaa gaucaagacc     1260

gucggcaacc gcgugaucag ccgguccagc ccgcuguucc aggaguucaa gaucuggcag     1320

auccugaaca acaucgaggu gaccgucgug gggaagaagc gcaagcggcg caagcucaag     1380

gagaacuaca gcgcgaucuu cgaggagcug aacgacgccg agcagcugga gcugaacggc     1440

ucccggcgcc ucugccagga ggagaaggag cugcuggccc aggagcuguu cgugcgggac     1500

aagaugacca agagcgaggu ccugaagcuc cuguucgaca acccccagga gcuggaccug     1560

aacuucaaga cgaucgacgg caacaagacc ggguacgccc ucuuccaggc cuacuccaag     1620

augaucgaga ugagcggcca cgagcccgug gacuucaaga agcccgucga gaagguggug     1680

gaguacauca aggcggucuu cgaccugcug aacuggaaca ccgacauccu cggguucaac     1740

uccaacgagg agcuggacaa ccagagcuac uacaagcugu ggcaccuccu guacuccuuc     1800

gagggcgaca acaccccgac ggggaacggc cgccugaucc agaagaugac cgagcucuac     1860

ggcuucgaga aggaguacgc caccauccug gccaacguga gcuuccagga cgacuacggg     1920

ucccugagcg ccaaggccau ccacaagauc cucccccacc ugaaggaggg caaccgguac     1980

gacguggcgu gcgucuacgc cggguaccgc cacuccgaga gcucccugac ccgcgaggag     2040

aucgccaaca aggugcucaa ggaccggcug augcugcucc ccaagaacag ccugcacaac     2100

cccguggucg agaagauccu gaaccagaug gugaacguga ucaacgucau caucgacauc     2160

uacggcaagc cggacgagau ccgcguggag cucgcccggg agcugaagaa gaacgccaag     2220

gagcgcgagg agcugacgaa guccaucgcg cagaccacca aggccaacga ggaguacaag     2280

acccuccugc agacggaguu cgggcugacc aacgugagcc ggaccgacau ccuccgcuac     2340

aagcuguaca aggagcugga guccugcggc uacaagaccc ucuacagcaa caccuacauc     2400

ucccgggaga agcuguucag caaggaguuc gacaucgagc acaucauccc ccaggcccgc     2460

cuguucgacg acagcuucuc caacaagacg cucgaggccc ggagcgucaa caucgagaag     2520

ggcaacaaga ccgcguacga cuucgugaag gagaaguucg gggaguccgg cgccgacaac     2580

agccuggagc acuaccugaa caacaucgag gaccucuuca aguccgggaa gaucagcaag     2640

accaaguaca acaagcugaa gauggccgag caggacaucc ccgacggcuu caucgagcgc     2700

gaccugcgga acacccagua caucgccaag aaggcccucu ccaugcugaa cgagaucagc     2760

caccgcgugg ucgcgacguc cggcagcgug accgacaagc ugcgggagga cuggcagcuc     2820

aucgacguga ugaaggagcu gaacugggag aaguacaagg cccuggggcu cgucgaguac     2880

uucgaggacc gcgacggccg gcagaucggg cgcaucaagg acuggaccaa gcggaacgac     2940

caccgccacc acgccaugga cgcccugacc guggccuuca cgaaggacgu guucauccag     3000

uacuucaaca acaagaacgc gucccuggac cccaacgcca acgagcacgc caucaagaac     3060

aaguacuucc agaacggccg ggccaucgcc ccgaugcccc uccgcgaguu ccgggcggag     3120

gccaagaagc accuggagaa cacccugauc agcaucaagg ccaagaacaa ggucaucacc     3180

gggaacauca acaagacccg caagaagggc ggcgugaaca agaacaugca gcagacgccc     3240

cgcgggcagc uccaccugga gaccaucuac ggcuccggga agcaguaccu gaccaaggag     3300

gagaagguga acgccagcuu cgacaugcgg aagaucgaga ccgucuccaa gagcgcguac     3360

cgcgacgccc uccugaagcg gcuguacgag aacgacaacg accccaagaa ggccuucgcc     3420

ggcaagaacu cccucgacaa gcagccgauc uggcuggaca aggagcagau gcgcaaggug     3480

cccgagaagg ugaagaucgu cacgcuggag gccaucuaca ccauccggaa ggagaucagc     3540

cccgaccuca agguggacaa ggugaucgac gucggggugc gcaagauccu gaucgaccgg     3600

cugaacgagu acggcaacga cgcgaagaag gccuucagca accucgacaa gaaccccauc     3660

uggcugaaca aggagaaggg caucuccauc aagcgcguga ccaucagcgg gaucuccaac     3720

gcccagagcc ugcacgucaa gaaggacaag gacggcaagc cgauccucga cgagaacggg     3780

cggaacaucc ccguggacuu cgugaacacc ggcaacaacc accacgucgc cguguacuac     3840

cgccccguga ucgacaagcg ggggcagcug gucguggacg aggccggcaa ccccaaguac     3900

gagcuggagg agguggucgu guccuucuuc gaggcgguga cgcgcgccaa ccucggccug     3960

ccgaucaucg acaaggacua caagaccacc gagggguggc aguuccuguu cagcaugaag     4020

cagaacgagu acuucgucuu ccccaacgag aagaucggcu ucaaccccaa ggagaucgac     4080

cuccuggacg uggagaacua cgggcugauc uccccgaacc ucuuccgggu gcagaaguuc     4140

agccugaaga acuacgucuu ccgccaccac cuggagacca cgaucaagga caccuccagc     4200

auccuccggg gcaucaccug gaucgacuuc cgcuccagca aggggcugga caccaucgug     4260

aagguccggg ugaaccacau cggccagauc guguccgucg gcgaguacug a              4311


<210>  2206
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2206
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccugguggac       60

gaggccgaga acaaggacga gcgguccagc aucgucaagc ucggggugcg cgugaacccc      120

cugaccgucg acgagcugac gaacuucgag aagggcaagu ccaucaccac caacgcggac      180

cggacccuca agcgcgggau gcggcgcaac cugcagcggu acaagcugcg ccgggagacg      240

cucauggagg ugcugaagga gcacaagcug aucaccgagg acaccauccu cagcgagaac      300

ggcaaccgca ccacguucga gaccuaccgg cugcgcgcca aggccgccac cgagaagauc      360

ucccuggagg aguucgcccg ggugcuccug augaucaaca agaagcgcgg cuacaagagc      420

ugccggaagg cgaagggggc cgaggagggc acccugaucg acgggaugga caucgcccgc      480

gagcucuaca acaacaaccu gacgccgggc gagcugugcc uccagcugcu ggacgccggg      540

aagaaguacc uccccgacuu cuaccggucc gaccugcaga acgagcugga ccgcaucugg      600

gagaagcaga aggaguacua ccccgagauc cucaccgagg uccugaagga ggagcugcgg      660

ggcaagaagc gcgacgcggu gugggccauc ugcgccaaga gcuucgugug ggaggaguac      720

uacaccgagu ggaacaagga gaagggcaag accgagcagc aggagcggga gcacaagcuc      780

gaggggaucu acuccaagcg caagcgcgac gaggccaagc gggagaaccu gcaguggcgc      840

gucaacggcc ugaaggagaa gcucagccug gagcagcugg ugaucgugcu ccaggagaug      900

aacauccaga ucaacaacuc cagcggguac cugggcgcca ucuccgaccg gagcaaggag      960

cuguacuuca acaagcagac ggucggccag uaccagaugg agaugcucga caagaacccc     1020

aacgcguccc ugcgcaacau gguguucuac cggcaggacu accuggacga guucaacacc     1080

cucugggaga agcaggccga guaccacaag gagcugaccg gggagcugaa gaaggagauc     1140

cgcgacaucg ugaucuucua ccagcggcgc cucaagagcc agaagggccu ggucggguuc     1200

ugcgaguucg agucccggca gaucgaggug gagaucgacg gcaagaagaa gaucaagacc     1260

guggggaacc gcgucauccc gcggagcagc ccccuguucc aggaguucaa gaucuggcag     1320

auccucaaca acaucgaggu gacggugguc ggcaagaagg gcaagcgccg gaagcagaag     1380

gacaacuccc ccacccuguu cgacgggcug gacgacaccg agcagcugga gcucaacggc     1440

agccgcuacc uguaccagga ggagaaggag cugcucgccc aggagcuguu cgugcgggac     1500

aagaugacca aguccgacgu gcugaagcuc cuguucgaca acccccagga gcuggaccuc     1560

aacuucaaga cgaucgacgg gaacaagacc ggcuacaccc uguuccaggc cuacagcaag     1620

augaucgaga uguccgggca cgagccgguc gacuucaaga agaccgugga gaaggucgug     1680

gagcacguga aggccgucuu cgaccugcuc aacuggaaca cggacauccu gggcuucaac     1740

agcaacgagg agcuggacaa ccaguccuac uacaagcucu ggcaccuccu guacagcuuc     1800

gagggcgaca acacccccac cgggaacgcg cgccuggugc agaagaucac cgagcuguac     1860

ggcuucgaga aggaguacgc caucauccug gccaacguga cguuccagga cgacuacggg     1920

ucccucagcg ccaaggccau ccacaagauc cugccccacc ugaaggaggg caaccgguac     1980

gacgucgcgu gcgccuacgc cggguacaag cacuccgaga gcucccucac caaggaggag     2040

aucgccaaca aggcccugaa ggaccgccug acccuccugc ccaagaacaa ccugcggaac     2100

ccgguggugg agaagauccu caaccagaug gucaacguga ucaacgcgau caucgacacc     2160

uacggcaagc ccgacgagau ccgcguggag cuggcccgcg agcugaagaa gaacgccaag     2220

gagcgggagg agcucacgaa guucaucgcc gagaccaccc gcaagcacga ggaguucaag     2280

auccugcugc agagcgaguu cggccucacc aacgucuccc ggaccgacau ccugcgcuac     2340

aagcuguaca aggagcucga gagcugcggg uacaagacgc uguacuccaa caccuacauc     2400

ccccgggaga agcuguucag caaggaguuc gacaucgagc acaucauccc ccaggcgcgc     2460

cucuucgacg acuccuucag caacaagacc cuggaggccc gguccaucaa ccuggagaag     2520

ggcaacaaga ccgccuacga cuucgugaag gagaaguucg gggagagcgg cgccgacaac     2580

agccucgacc acuaccugaa caacaucgag gaccuguuca aguccggcaa gaucagcaag     2640

acgaaguaca acaagcucaa gauggccgag caggacaucc cggacggguu caucaaccgc     2700

gaccugcgga acacccagua caucgcgaag aaggcccugu ccaugcucaa cgagaucagc     2760

cgccgggugg ucgccaccuc cggcaccauc acggacaagc ugcgccagga cuggcagcug     2820

guggacguga ugaaggagcu caacugggag aaguacaagg cccuggggcu ggucgaguac     2880

uucgaggaca aggacggccg gcagaucggg cgcaucaagg acuggaccaa gcggaacgac     2940

caccgccacc acgccaugga cgcgcucacc guggccuuca ccaaggacau cuucauccag     3000

uacuucaaca acaagaacgc cagccugaac uccaacacga acgaguacgc caucaagaac     3060

aaguacuucc agaacggccg ggccaucgcg cccauccccc ugcgcgaguu ccggguggag     3120

gccaagaagc accucgagaa cacccugauc agcaucaagg ccaagaacaa ggucgugacc     3180

ggcaacauca acaagaccaa gaagcgcggg ggcgugaaca aguaccugca gcagacgccc     3240

cgggggcagc uccaccugga gaccaucuac ggcuccggga agcaguaccu gaccaaggag     3300

gagaagguca acgccagcuu cgacaugaag aagaucgcga ccguguccaa gagcgccuac     3360

cgcgacgugc uccugaagcg ccugcacgag aacgacaacg acccgaagaa ggccuucacg     3420

ggcaagaacu cccucgacaa gcagcccauc uggcuggaca aggagcagac ccggaagguc     3480

cccgagaagg ugaagaccgu gacccuggag acgaucuaca ccguccgcaa ggagaucagc     3540

cccgaccuca agguggacaa ggugaucgac gccggcgucc ggaagauccu gaccgggcgc     3600

cugaacgagu acggcaacga cgccaagaag gcguucucca accucgacga gaacccgauc     3660

uggcugaaca aggagaaggg gaucagcauc aagcggguga ccaucuccgg caucagcaac     3720

gcccagagcc ugcacgugaa gaaggacaag gacgggaagc ccauccucga cgaggacucc     3780

cgcaacaucc ccgucgacuu cgugaacacg ggcaacaacc accacguggc cgucuaccgg     3840

cgccccguga ucgacaagcg gggccagcug gugaucgacg aggccgggaa cccgaaguac     3900

gagcuggagg aggucguggu gagcuucuuc gaggccguca cccgcgcgaa ccucggccag     3960

cccaucaucg acaaggacua caagaccucc gagggguggc aguuccuguu cagcaugaag     4020

cagaacgagu acuucguguu ccccaacgag aagaccggcu ucaacccgaa ggagaucgac     4080

cugcucgacg ccgagaacua cggggugauc ucccccaacc uguuccgggu ccagaaguuc     4140

gcccacaaga acuacguguu ccgccaccac cuggagacga ccaucaagga caccagcucc     4200

auccucaagg gcaucaccug gaucgacuuc cggagcucca agggccugga cgccaucgug     4260

aagguccgcg ugaaccacau cgggcagauc gucagcgugg gcgaguacug a              4311


<210>  2207
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2207
augaaguaca ucaaguacau ccugggccuc gacaucggga gcaacuccau cggcugggcc       60

cugauccagc uggaccuccu gcagaagacc aagcgcauca ucgccgcggg gagccggauc      120

auccccaugg accagaaggu gcuguccgac uucggcaagg ggaucaccau cagcccgacg      180

gcccagcgca ccuccuaccg gaacgcccgc cacugccggg agcgccaccu ccugcggcgc      240

gagcggcugc accgcguccu ccacauccug ggcuuccugc ccacccacua cgacaagcag      300

aucgacuuca gcaagcggua cggcaaguuc guggacaaca ccgagcccaa gaucgccuac      360

aacgagggga aguuccucuu cauggacucc uuccaggaga ugcugagcga cuucaagucc      420

caccagcccg accuguucua cacgaagaag aacggcgagg agagcaagau cccguacgac      480

uggaccaucu acuaccuccg caagaaggcc cugucccaga agaucagcaa ggaggagcug      540

gcguggauca uccucaacuu caaccagaag cggggguacu accagcugcg cgaggaggag      600

ggcaacaagg agaacgacuu cgagaucauc uccagcaaga ucaucuccgu gaucaagaag      660

gacaaggaca agaaguacaa caaguacugg uacgacauca uccuggagaa cgggcucguc      720

uaccaggccg ccuucuacaa cgacuucgug uacaacugga ucaaccagga ccgggaguuc      780

aucaucgagc gcaccagccu gaagaacggc gccgagaagg ugaagcuguc cuaccucccc      840

accuucgacg agaucgacaa gauggacaag aaggagcagg acaaguacua caagaagauc      900

aagaucaaga cggagaacag caucggcaac aagaccgucg ggugcuacau cuacgaccac      960

auccugcaga accccaacga gaagaucaag ggcaagcugg ugcggaccau cgagcgcaag     1020

uacuacaagg aggagcucaa ggcgauccug gagaagcaga ucgcccugca gcccgagcug     1080

uucaccgacc agcugcuggc cgacugcauc cgggagcucu acuccaagaa cgaggcccag     1140

cagcgcaacc uggccgcgcg ggacuucgug caccuccucg ucgaggacau caucuucuac     1200

cagcgcccgc ugcgccggca gaaguccacg cuggccaacu gcagccucga gagccgcagc     1260

uacaucgaca aggacuccca cacccggaag gaggcgcccc ugaaggugug ccccaagagc     1320

aaccccuacu accaggaguu ccgcgugcug caguggcucc agaaccugaa gaucuacgag     1380

aucgacuccg accaggaggu cacccaccag uucaucaaga cccuggagga caagcagcag     1440

cuguucgagu uccugauggc ccagaaggag aucgacugcg aggagcugcu caaguacuuc     1500

cugaaccaga cguacccgaa cgccaaggag aaggcccuca agagcgagcu gaagaagugg     1560

aaggacaccu accgguggaa cuacguguac gacaucgggg agaaguccag caagaaguac     1620

cccaugaacg agacccgcua cgagcucaag cggcaccugg agaagguggc caaccugccc     1680

gacgacuucc ucuccagcga ggucgaguac cugcuguggc accucaucua cuccgugaag     1740

gacaaggugg ccuacgagaa gggccugaag aaguucgccc agaagcacca ccuggacaag     1800

gacagcuucg ucgaguccuu caagaaguuc aaccccuacc cgagcgagua cggcuccuuc     1860

agcgagaagg ccauccgcaa gcuccugccc cugaugcggu ucggggcgca cugggacuuc     1920

aaccacaucg acaagaacac ccagaagcgc aucgaggacc ucaucacggg cguggagaac     1980

gaggagaucc ggaccauccu gcgcgagaag gccgagaagu accagcugga gaaggagacc     2040

gacuuccagg accucccccu guggcuggcc caguacaucg uguacaaccg gcacgccgag     2100

gccuccagcc ucgagaagug gaccuccguc aacgaccugg agacguaccu gaacgaguuc     2160

aagcagcaca gccuccgcaa ccccaucgug gagcaggucg ugauggagac ccugcgggug     2220

guccgcgaca ucuggcagca guacgggcag ggccaggcga gcuucuucaa cgagauccac     2280

aucgagcugg ggcgggagcu caagaagacc gccaaggagc gcgagaagcu guccaagcag     2340

aaccagaaga acgaggacau caaccugagc aucaaggaga agcucaagga gcuggagaag     2400

uacaacaccc gggccuacuc caucacgcac caggagaagu acaagcugug gcucgagcag     2460

aagcacauca gcccguacac cggccagauc aucucccccg agaaccuguu caccgacaac     2520

uacgagaucg agcacaucau cccccagagc cgcuucuucg acgacucccu gagcaacaag     2580

guggugugcg aguccaucgu caacaaggcc cccuacaagg accgccagcu cggccuggag     2640

uucaucaaga accagggcgg gcggaucgug aaggagcuga gcaaggagaa caagaccauc     2700

aagaucuuca cggaggagca guaccgcacc uucaucaagg agcacuacuc caacaacccg     2760

gagaagacca agaagcuccu gcuggaggag auccccgaga agaugguggc gcggcagaug     2820

aacgacacgu gcuacaucag caaguucauc uccgagaucc ucagcaagau cguccgcucc     2880

gacgagcagg acgagggggu gcacagcaag aacgugaucc ugugcaccgg caagaucacc     2940

uccauccugc ggcaggacug ggggcucaac gacaucuggg acaaccugau ccugccccgc     3000

uucgagcgga ugaacaaccu ccugcagaag gagaaguuca ccaucuacag caucgagaag     3060

cagaagaaga uccccgucgu gccgaucaac gaccaguuca agagcuucca gaagaagcgc     3120

aucgaccacc ggcaccacgc cauggacgcc aucaucaucg ccugcaccac ccgcgagcac     3180

aucaacuaca ucaacaacca gcacgcccug gagaaggaga aggacaagaa ggagaagcag     3240

gaggagcggg acaagcuccg cgagaagcug ugcaucaaga aguacaacaa ccgguccgag     3300

ggcaacuaca gcuggaucuu caagaagccc ugggacacgu ucacccagga ggugcgcguc     3360

gcgcuggagg acaucguggu guccuucaag cagaaccucc gggucaucaa caaggccacc     3420

aacuacuacg agcgcugggu ggaggagaac ggcaaguaca ucaagaagau ggugaagcag     3480

gaggggacca acugggccau ccggaagccc cugcacgagg agacgaucag cggcaagauc     3540

uaccuggacc gcgagaagau caccaagaac gacauccuca ccgccacccg gaagacgguc     3600

gacuccagcu ucgacgacaa gcgcaucaag aagaucaccg acaccgggau ccagaagauc     3660

cugcugaacu accucaagua caagggcucc ccggagaucg ccuucagccc cgaggggcug     3720

gaggagcuga acaagaaccu cuccaucuac aacgacggca agccccacaa gcccaucuac     3780

aaggugcgga ucuacgagaa gggcagcggg cgcuucgcgc ugggcgagaa ggggcgcaag     3840

uacaagaagu acgugcaggg cgccuccaac cuguacuucg gcaucuacca gggggccaag     3900

cggagcuucg ccaccauccc gcucaacgag gucaucgagc gccagaagca gggccugccc     3960

uccgugcccg aguacaacga gaagggggac cugcuccugu ucagccuguc ccccaacgac     4020

cucguguacg ucccgaucga gggcgagauc aucgagaaca ucgacuuccg gaaccugagc     4080

aaggagcaga aggagcgcau cuacaacgug aacgacuucu ccagcacgug cuacuucacc     4140

cccaaccgga ucgccaaggc gaucuucccc aaggaggugg accugcagcg caagggggac     4200

aagcucuccg gcagcuacga caccaagacc gccagccugg agggcaucca gaucaaggag     4260

gucugcauca agcugaaggu ggaccggcuc gggaacaucu ccaaggccug a              4311


<210>  2208
<211>  4314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2208
augaaguaca ucaaguacau ccugggccuc gacaucggga gcaacuccau cggcugggcc       60

cugauccagc uggaccuccu gcagaagacc aagcgcauca ucgccaccgg gagccggauc      120

auccccaugg accagaaggu gcuguccgac uucggcaagg ggaucacgau cagcccgacc      180

gcgcagcgca ccuccuaccg gaacgcccgc cacugccggg agcgccaccu ccugcggcgc      240

gagcggcugc accgcguccu ccacauccug ggcuuccugc ccacccacua cgacaagcag      300

aucgacuuca gcaagcggua cggcaaguuc guggacaaca cggagcccaa gaucgccuac      360

aacgagcgca aguuccucuu cauggacucc uuccaggaga ugcuggccga cuucaagauc      420

caccagcccg accuguucua caccaagaag aacggggagg agagcaagau cccguacgac      480

uggaccaucu acuaccuccg gaagaaggcc cugucccgca agaucagcaa ggaggagcug      540

gcguggauca uccucaacuu caaccagaag cggggcuacu accagcugcg cgaggaggag      600

gaggagaaca aggagaacga cuucgagauc aucuccagca agaucaucuc cgugaucaag      660

aaggacaagg acaagaagua caacaaguac ugguacgaca ucauccugga gaacgggcuc      720

gucuaccagg ccgccuucua caacgacuuc guguacaacu ggaucaacca ggaccgggag      780

uucaucaucg agcgcaucag ccugaagaac ggcaccgaga aggugaagcu guccuaccuc      840

cccacguucg acgagaucga caagauggac aagaaggagc aggacaagua cuacaagaag      900

aucaagauca agaccgagaa cagcaucggg aacaagaccg ucggcugcua caucuacgac      960

cacauccugc agaaccccaa cgagaagauc aagggcaagc uggugcggac caucgagcgc     1020

aaguacuaca aggaggagcu caaggccauc cuggagaagc aggucgcgcu gcagcccgag     1080

cuguucacgg accagcuguu cgccgacugc auccgggagc uguacuccaa gaacgaggcc     1140

cagcagcgca accucgccgc ccgcgacuuc gugcaccugu ucguggagga caucaucuuc     1200

uaccagcgga gccugcgccg gcagaagucc acccucgcga acugcagccu ggagagccgc     1260

uccuacaucg acaaggacag ccacacccgg aaggaggccc cgcugaaggu gugccccaag     1320

uccaaccccu acuaccagga guuccgcguc cuccaguggc ugcagaaccu gaagaucuac     1380

gagaucgaca ccgaccagga ggugacgcac gaguucauca agacccucga ggacaagcag     1440

caguucuucg acuuccugau gaaccagaag gagaucgacu gcgaggagcu gcucaaguac     1500

uuccugaacc agaccuaccc caacgcccgg gagaaggccc ucaagagcga gcucaagaag     1560

uggaaggaca ccuaccgcug gaacuacgug uacgacaucg gggagaaguc cagcaagaag     1620

uacccgauga acgagacgcg guacgagcug aagcgccacc uggagaaggu cgccaaccug     1680

cccgacgacu uccucuccag cgagguggag uaccugcugu ggcaccucau cuacuccgug     1740

aaggacaagg ucgcguacga gaagggccug aagaccuucg cccagaagca ccaccuggac     1800

aaggacagcu ucguggaguc cuucaagaag uucaagcccu accccagcga guacgggucc     1860

uucagcgaga aggccauccg gaagcuccug ccgcugaugc gcuucggcuc cuacugggac     1920

uucaaccaca ucgacaagaa cacccagaag cggaucgagg accucaucac cggcguggag     1980

aacgaggaga uccgcacgau ccugcgggag aaggccgaga aguaccagcu ggagaaggag     2040

accgacuucc aggaccuccc ccuguggcug gcccaguaca ucgucuacaa ccgccacgcg     2100

gaggccagcu cccucgagaa guggaccagc gugaacgacc uggagaccua ccugaacgag     2160

uucaagcagu acucccuccg gaaccccauc guggagcagg ucgugaugga gacgcugcgc     2220

gucgugcggg acaucuggca gcaguacggg cagggccagg ccagcuucuu cgacgagauc     2280

cacaucgagc uggggcgcga gcucaagaag accgccaagg agcgcgagaa gcugagcaag     2340

cagaaccaga agaacgagga caucaaccug uccaucaagg agaagcucaa ggagcuggag     2400

aaguacaaca cccgggccua cagcaucacc caccaggaga aguacaagcu guggcucgag     2460

cagaagcaca ucucccccua cacgggccag aucaucagcc cggagaaccu guucaccgac     2520

aacuacgaga ucgagcacau caucccccag ucccgcuucu ucgacgacag ccuguccaac     2580

aagguggucu gcgagagcau cgugaacaag gcgcccuaca aggaccggca gcucgggcug     2640

gaguucauca agaaccaggg cggccgcauc gugaaggagc uguccaccga gaacaagacc     2700

aucaagaucu ucacggagga gcaguaccgg accuucauca aggagcacua cagcaacaac     2760

cccgagaaga ccaagaagcu ccugcuggag gagaucccgg agaagauggu cgcccgccag     2820

augaacgaca cgugcuacau cuccaaguuc aucagcgaga uccucuccaa gaucgugcgg     2880

agcgacgagc aggacgaggg ggugcacucc aagaacguca uccugugcac cggcaagauc     2940

accagcgccc ugaagcagga cuggggcgug aacgacgugu ggaacgaccu cauccugccc     3000

cgcuucgagc ggaugaacga ccugacccag uccacccucu ucaccacgua caacgagaag     3060

uaccagaagu accugcccac cguccccaug gaguacagca acggguucca gaagaagcgc     3120

aucgaccacc ggcaccacgc cauggacgcg aucaucaucg ccugcgccac ccgcgagcac     3180

aucaacuaca ucaacaacca gcacgcccug gagaagggga aggacaagaa ggagaagcag     3240

aaggagcacg acaagcuccg ggagaagcug ugcaucaaga aggacaacaa gggcagcgag     3300

gagaacuacu ccuggaucuu caagaagccg ugggacaccu ucacgcagga ggugcaggcc     3360

gcgcuggagg gcaucguggu cagcuucaag cagaaccucc gcgugaucaa caaggccacc     3420

aacuacuacg agcggugggu ggaggagaac gggaaguaca ucaagaagau ggucaagcag     3480

gagggcacca acugggccau ccgcaagccc cugcacgagg agaccaucuc cgggaagauc     3540

uaccuggacc gggaggagau cacgaagaac gacauccuca ccgccacccg caagaccgug     3600

gacagcuccu ucgacgagaa gcggaucaag aagaucacgg acaccggcau ccagaagauc     3660

cugcugaacu accucaagua caaggggagc cccgagaucg ccuucucccc ggagggccug     3720

gaggagcuga acaagaaccu cagcaucuac aacgacggca agccccacaa gcccaucuac     3780

aaggugcgca ucuacgagaa gggguccggc cgguucgcgc ugggggagaa gggccgcaag     3840

uacaagaagu acguccaggg cgcccccaac cuguacuucg ggaucuacca gggcgccaac     3900

aagcgcagcu ucgccaccau cccgcucaac gaggugaucg agcggcagaa gcaggggcgc     3960

cccuccaucc ccgaguacaa cgagaagggc gacccccugc uguucagccu cuccccgaac     4020

gaccuggugu acguccccgu ggagggggag aucaucgagg acaucgacuu ccagaaccug     4080

agcaaggagc agaaggagcg gaucuacaac gugaacgacu ucuccagcac gugcuacuuc     4140

acccccaacc gcaucgccaa ggcgaucuuc cccaaggagg ucgaccucca gcggaagggc     4200

gacaagcugc cgggcuccua cgacaccaag accgccagcc uggaggggau ccagaucaag     4260

gaggugugca ucaagcucaa gaucgaccgc cugggcaaca ucuccaaggc cuga           4314


<210>  2209
<211>  4317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2209
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucaag       60

cagaacuucg agaacaagua cggggagauc cucggcaugg ggucccgcau cauccccaug      120

agccaggaca uccugggcga guucggcaag gggaacuccg ugagccagac ggccgcgcgg      180

accgacuacc gcgccacccg gcgccugcgg gagcgcuucc uccugcggcg cgagcggcug      240

caccgcgucc ucaacgugcu gaacuuccug ccggagcacu acgccuccca gaucgacuuc      300

gacaagcggu ucggcaaguu caagguggag accgagccca agcucgccug gaagaacauc      360

gacgggaagu ucagcuuccu guuccagacg uccuucaacg agaugcugga ggacuucaag      420

gcccacggcc aggaccucaa gguccccuac gacuggacca ucuacuaccu gcgcaagaag      480

gcgcugagcc agaagaucga gaaggaggag cucgugugga uccugcugaa cuucaaccag      540

aagcgggggu acuaccagcu ccgcggcgag gaggaggagg agaaccccaa caagcugauc      600

gaguucuacu cccugaagau caucgacgug cucgccgacg agccgcagaa gggcaagagc      660

gacaucuggu acucccuggu ccuggagaac ggguggaucu accggcgcag cuccaagacc      720

agccuccugg acuggaagga caagauccgg gacuucaucg ugaccacgga ccugaacgac      780

gacggcuccg ugaagaccga caaggagggg aacgagaagc gcagcuuccg ggcccccggc      840

gagaacgacu ggacccucgu caagaccaag acggagcagg agaucgaccg cucccggaag      900

accgugggca acuacaucua cgagacccug cugcagaacc ccaagcagaa gaucaagggg      960

aagcucgugc gcaccaucga gcgcaaguuc uacaaggagg agcugaagcg gauccuggag     1020

aagcagaagg aguuccaccg cgagcucgag agcaacgacc uguacaacga cugcauccgg     1080

gagcuguacc gcaacaacga ggcccaccag cucacgcugu ccaagaagga cuucguccac     1140

cuguucaugg aggacaucau cuucuaccag cggccccuca agagccagaa guccagcauc     1200

agcaacugca cccuggaguu ccgcaaguac aaggacgaga acggcaucga gcacacccag     1260

uuccugaagg cgaucccgaa guccaacccc uacuaccagg aguuccggau cuggcagugg     1320

aucuucaacc ucaacaucua caagaaggac aacgacgaca acgugaccaa ggaguuccug     1380

aacacgaccc gcgacuucga gaaccuguuc gaguuccuca acaaccggaa ggagaucgag     1440

cagaagcccc ugcugaaguu ccugcucgag cagaaggagu ucaaggggaa gcugcugaac     1500

gccgagaccg agaaguuccg cuggaacuac guggaggaca agaaguaccc cugcaacgag     1560

acccggacga ugaucagcga gcgccucaag aaggucgaga acaucuccga cgacuuccug     1620

acccgggaga uggagcagaa gaucuggcac aucaucuaca gcgugaacga caagaccgag     1680

uacgagaagg cccugaaguc cuucgccgag aagaacaacc ucgacgagaa cagccuguuc     1740

gaggccuuca agaagcugcc gcccuucaag uccgaguacg gcagcuucuc cgagaaggcg     1800

aucaagaagc uccugccccu gaugcgccuc gggaaguacu ggagcuacgc caagaucgac     1860

cuguacucca agaaccggau ccagaagauc aucaccggcg aguucgacga gaacaucaag     1920

gaccgcgugc gggagaaggc cauccgccug acggccgaga acgacuucca gggccuccag     1980

cuguggcugg cccaguacau cgucuacggg cggcacagcg aggcguccau gaucggcaag     2040

uggaacagcg ccgacgaccu cgaggaguuc cugaaggagu ucaagcagca cucccugcgc     2100

aaccccaucg uggagcaggu caucaccgag acccuccgca ucgugaagga caucuggcug     2160

aaguacggga acggcgccaa ggacuucuuc aacgagaucc acaucgagcu ggggcgggag     2220

augaagcaga ccaaggacga gcgcgccaac gccacgaaga ccaucaccga gaacgagaac     2280

accaaccucc ggaucaaggc gcugcuggcc gagaugauga acgaccacag cguggagaac     2340

gugcgcccgu acucccccau gcagcaggag auccucaaga ucuacgagga cggcauccuc     2400

aagagcgaca ucgagaucga cgacgacauc cugaagauca gcaagacggc ccagcccucc     2460

agcuccgacc ugaagcggua caagcugugg cuggagcaga aguacaagag ccccuacacc     2520

ggccagauca ucccgcucaa caagcuguuc acccccgagu acgagaucga gcacaucauc     2580

ccccaguccc gcuacuucga cgacagcuuc uccaacaaga ucaucugcga gagcgccguc     2640

aacaagcuga aggacaacua caucgggcuc ggcuucauca agcaguucgg gggcaccauc     2700

aucgagugcg gguucggcaa gcgggugaag gucuucgagg ugggcgagua cgaggaguuc     2760

gugaagaagc acuacgccaa caaccgcggg aagcggaaca agcugcugcu cgaggagauc     2820

cccgagaaga ugaucgagcg ccagaugaac gacacccggc acaucuccaa guacaucagc     2880

ggcauccugu cccacaucgu ccgcguggag gacgggacgg acgagggcgu gaacagcaag     2940

aacgucaucc cgggcaacgg gaagaucacc acccagcugc ggcaggacug gggccucaac     3000

gacgugugga acgagcugau ccugccccgc uucgagcgga ugaaccagcu caccaacucc     3060

acggacuuca ccagcuggaa cgagaaccac cagaaguacc ugcccaccgu gcccgucgag     3120

uucuccaagg gguucagcaa gaagcgcauc gaccaccggc accacgcgcu ggacgcccuc     3180

gugaucgccu gcgccaccaa ggaccacgug aaccugcuga acaaccaguc cgccaagagc     3240

gcgacgaagc gcuacgaccu caagaagaag cugaugaagu ucgagaagac cgucuacaag     3300

gacccgcaga ccgagaagcg gaucgagcgc gaggugccca agcacuuccu gaagcccugg     3360

gagaccuuca cgguggacgc caagaaccgg cucgagacca ucaucgucag cuucaagcag     3420

aaccugcgcg ugaucaacaa ggccaccaac uacuacgaga aguacgugga gaaggacggc     3480

gucaugauga aggagcgcgu ggagcagacc gggacgaacu gggccauccg gaagccgaug     3540

cacaaggaga ccguguccgg caagaucgac cugcccuggg ucaaggugcc caagggcaag     3600

auccucaccg cgacccgcaa gagccuggac acguccuucg accugaaggc caucgccagc     3660

aucaccgaca ccgggaucca gaagauccuc aagaacuacc uggaguucaa ggaguccccc     3720

gagcuggccu ucagcccgga gggcaucgag gacaugaaca agaacaucga gaaguacaac     3780

gggggccggc cccaccagcc caucuccaag gugcgcgucu ucgagcucgg gagcaaguuc     3840

caggugggcc agaccggcaa caagaaggac aaguacgugg aggccgcgaa ggggacgaac     3900

cuguucuucg ccaucuacga ggacaagaag ggcaagcggu ccuacgagac caucccccug     3960

aacgagguca ucgagcgcca gaagcagggg cucagcgugg uggaccugaa gaacgucaac     4020

gacuucuucc ugugcccgaa cgaccucgug uacauccccu ccggcgacga guucgagaac     4080

cgggggagca ucgagaucga gaacaucacc aaggagaagu ccgagcgcuu cuacaaggug     4140

gucagcuucu ccggcagcca gaucuucuuc gugcggcacg acaucgccgu guccaucguc     4200

aacaagggcg aguucagcac ccugaacaag auggagcgcg ccaucgacgg guccauggug     4260

aaggagaucu gcaucaagcu gaagaucgac cggcucggcg acgugcugaa ggccuga        4317


<210>  2210
<211>  4317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2210
auggugaaga agggcuacac caucgggcug gacaucggca ccacgagcgu cgggugggcc       60

gugaccgacg acgacuucga gcucgugaac aagaagaugu ccaucaagga caagagcggc      120

uccaccauca agcgcguccg gaagaaccug uggggcgugc gccuguucga cgaggccaag      180

accgcggaga acacgcggcg cacccgcggg gugcggcggc gcauccggcg ccggaaggag      240

cgccucaacu accugcaggg caucuucagc uccgagaugg ccaagaugga cgagaacuuc      300

uucaugcggc uggaggagag cuucuucaag cucuccgaca agagcgagca gaacaaguuc      360

cccuacgccc uguucaagaa cgagaucgag gagaaggccu acuaccgcca guucccgacc      420

aucuaccacc uccggaagca ccucauggag cacgaggagu ccgaccugcg caagaucuac      480

cuggggcucc accacauccu gaaguaccgg ggccacuucg uccaggaggg gcagaagaug      540

gacguggcca acaucgacau ccagggcaac cugaaggagc uguucaacgc gcugaacgag      600

aacuucagca acaaggagga cgacuucgag caggagcgca ugaaggacgg ggugaagcgg      660

gccaaggucc uguucgacac cgagaagcuc gccgccgugg aggagauccu gaaggagaag      720

aucuccaaga gcuggaaggu gaacaagauc uccaagggca uccuggcgga gaagaaggac      780

cuccccaucu ucaaggccau cgucggcaac aagaucgacc uggccgggau cuucgacugc      840

cccgacuacg ccgagaaggu gaacgagagc aucaaggagc ugaaggacaa gguguacuuc      900

aacaaggagg acuucgagga gacguucgag aagcucuccc agcugcugaa ggaggaggag      960

cucgagaucc ugauccuggc ccgcaagugc uacgaggcgg uccuccuggc cgacaucaug     1020

cagggcgagc agagccuguc cgacgccaug guggccaagu acaacgacca caagaaggac     1080

cucgugaucu ucaagaaguu cgucaucgac uacuucagcg aggacgugua cuucgaccug     1140

uucaagaaca cccagcuggc cgggaacuac cacuccuacg ugaagggcga gcggaagggc     1200

aacaaguuca accacgcgag ccucgacgac uucuacaagu ucgucaagaa ggagcuggac     1260

aagaaggccg aggagcugag cgagcacccc guguacaagc agauccucca gaagaucgag     1320

cuggagaccu uccugccgaa gcagcgcaug uucaagaacg gggccauccc cuaccaggug     1380

caccuccacg agcugcgcgc caucaucaag aagcaggaga agcgguuccc cuuccuggcc     1440

gagaagauca ucaucaagga caaccagaag gacaucuccg aguacaagau cgagacccuc     1500

augaaguucc gcauccccua cuacgucggc ccgcugaucg aggcgcggga gggggagcug     1560

ggcaagaagc gcacgaacua cagccgguuc gccuggaugc agaagucccc cgagagccag     1620

aacaucggga ucacccccug gaacuuccac uccguggucg acaaggacgc cagcgccucc     1680

gaguucaucg agcgcaugac cggcuucugc agcuacuucc ccgaggagcc ggugcucccc     1740

aagaacuccc ugaucuacca gcaguucacc guguuccagg agcugagcau cgucggcuac     1800

uacgacgagc agaaccggaa gcacuaccug gacggggaga cgcgccggcg caucuucgag     1860

gaccucuuca agaagaaccg gaccgugucc gccggccugc ugcgcaagug gcucgugugc     1920

aaccagcgga ccagccagga ggagaaggag auccgccugu ucgggcugga ccucaagaag     1980

aacggccugg gguccggcaa cuucaacacc aaguacacga ccuaccugga ccucaaggac     2040

gcgggcauca ccgagaagca gcugaagcag cacaaggacu ucuucgagaa gaucgucaag     2100

uaccagacca ucuucgagga caagaagauc cugaagcggc gcaucauccu ccugaacgag     2160

gagcagcccc ugcucagcga ggagcagauc aagaaccugg ccaagaagca cuacacgggg     2220

uggggcaacc ugucccucaa gcugaucgac gggauccagg acaagggcaa gaccauccug     2280

gacuaccucg agacccagga cuacaaccag aaccugaugc agcugaugaa cgacgagaac     2340

aagagcuucg agaugcagau cgagcaggcc cgggccggga aggugcaguu cggcaagauc     2400

gacuacuccu ucguggagca gcucgcgggc agccccgcca ucaagcgcgg gaucuggcag     2460

ucccugcaga ucgucgagga gcugguggcc uuccucggca aggagaacau caagaagauc     2520

gugaucgaga uggcccggga gaaccagagc agcaagcgca ccaagucccg ccagaagcag     2580

aucgaggccc uguaccagaa ggucacgggg gaccugaaga acgagcagga gaagaucgac     2640

gugaugcaga acuucgacaa cgagcgggug uuccucuacu accugcagaa cggcaagugc     2700

augcacagcg gcgagaagcu ggagcucucc cagcugagcg aguacgagau cgaccacauc     2760

aucccgcaga ccuacaucaa ggacgacucc uucgacaaca agguccuggu gacccgcaac     2820

gcgaaccagc agaagggcgg ggacgugccc agcuacgacg ucaucgacaa gaugaagucc     2880

uacugggaga agcucaagga gaacaaccug aucagcgaga agaaguucgc caaccugacc     2940

aaggggaagu ucgacgacca gcagcucucc ggcuucauca accggcagcu gguggagacg     3000

cgccagauca ccaagaacgu ggccaucauc cuggacaaga ucuacaacga cgaggccaag     3060

aacgagaagg aggucgccau caucacccuc aagagcgcgc ugaccuccca guaccggcag     3120

gggaucaucu acgugcccaa cgacgacuuc gacgagcuga agcucgugag caagacgaac     3180

ucccgccuga aggaggucaa gcugcacgac ggcuucuaca aggugcggga gaucaacgac     3240

uaccaccacg cccacgacgc cuaccucaac aacguggucg ccaccuaccu guacgaggcc     3300

augcccgacc ugcgcccccu cuucguguac ggcgagcaca gccgggagau ccacaagcgc     3360

uuccugggga aguacgcgac cacccggaag ggccaguuca agcagcugcu cguggacaug     3420

caggacgagu acuggaugcg caaggacaag gaggggaacu ucaucgacgg cgagauccug     3480

uggcggcgcg acgagguccu gcagaucgug aagaaggugc ucgacuaccg gaacauccag     3540

aucaccaaga agcuggagau gauggucacc gggggcgagg agaaguccuu cuaccaggac     3600

agccgcugcc ggaaggacaa gacggccauc ccguacaaga ccaacuggga ccccuccaag     3660

uacggcgggu ucaagagccc ccgcaccgcc uacgccaucc cggugaucaa gaagaacaag     3720

cccaagcugg agcccgugcu ccuggucaac cggagcaagg uggagacgga cggccaguuc     3780

gacugcgaga aguucaagga gcugaacccc acccucaaga uccuggggca gccgaucugg     3840

aaguaccagu ccuucgagau guucgacggc accaagcgcu acaucgcgag cgccuccgag     3900

auccacaagg ggaaccagau caaguucagc caggagcaca ugaaguucau cuccugcugc     3960

cagaaguacg acgaggugaa gaacaagcug gccuucgacu ucgucaacga gagcaagcac     4020

cucuuccagg agauccugac cgccaucaag gacaacaucc accgguaccg ccugaucgcc     4080

gagggcaucg aggugcaccg caaguucucc aacaugcucg agaacaucga gaagcugagc     4140

acgcaggagc ugguggacaa ccuccuggac gcgaucaaca ucaccuccgu cggcaccacc     4200

aacaacgccu ucgggcgggu gcgcuacaag ggcagcuacc ugaacgagau cugguccgcc     4260

acgcucaucc accagagcgu gaccgggcug uacgagaccc gggagaagcu gaucuga        4317


<210>  2211
<211>  4323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2211
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc ccugguggag       60

caggacuucg agaacaagca ggggcucauc cugggcaugg ggucccgcau cauccccaug      120

gacgccggca ucaucggcaa guucgcggag gggagcucca ucagccagac ggccgagcgg      180

accggcuacc gcuccauccg gcgccugcgg gagcgccacc uccugcggcg cgagcggcug      240

caccgcgucc ucaacauccu gaaguuccug ccggagcacu acgccgccga gaucgacuuc      300

gagaagcggu ucgggcaguu ccucgccgag accgagccca agcuggcgug gaagaagucc      360

gccgagggca aguucgaguu ccuguuccag cgcagcuucg acgagauggu ggccgacuuc      420

aaggccaacg ggcaggacau caagaucccc uacgacugga ccaucuacua ccuccggaag      480

aaggcgcuga cgcagaagau cagcccccag gagcuggccu ggaucauccu caacuucaac      540

cagaagcgcg gcuacuacca gcugcggggc gaggaggagg aggagaaccc gaacaagaag      600

guggaguucu acucccugaa gaucgucgac gugguggccg acgagcagcc caacaagaag      660

ggggacaccu gguacagccu ccaccuggag aacggcuggg ucuaccgccg guccagcaag      720

aucccccugu acgacuggaa ggacaaggug cgcgacuuca ucgugaccac cgacaucaac      780

gaggacgggu ccgagaagcu cgacaaggac ggcgagguca agcggagcuu ccgcgccccc      840

aaggaggacg acuggacgcu gaucaagaag aagaccgagc aggagaucga gcaguucaac      900

cagaccgugg gcuccuacau cuacgaggcc cugcucgaca acccgaccca gaagauccgg      960

gggaagcugg ugcgcacgau cgagcgcaag uucuacaaga gcgagcugaa gaagauccuc     1020

gagaagcaga ucgagcugca gcccgagcug uucaccgagg accucuacaa cgcgugcgcc     1080

cgggagcugu accgcuccaa cgucgcccac cagcagcagc ugagcucccg ggacuucgug     1140

uaccucuucc ugaacgacau caucuucuac cagcgccccc ugcggagcca gaagucccuc     1200

aucagcaacu gcagccugga guuccgcacc aucaaggaca aggacggcaa cgggcagauc     1260

aucuaccuga aggccauccc caaguccaac ccguacuacc aggaguuccg gguguggcag     1320

uggcucuaca accugaagau cuucaccaag gaggacgaca aggacgucac gaaccaguuc     1380

aucaacaagg uggaggaccu ggaggcccuc uucgaguucc ugaugaacca gaaggaggug     1440

aaccacaccg acauccugac cuaccucauc gagcccaucg ucaagagcaa guaccccaac     1500

gcgaagggca aggccuucaa ggacgagcug aagaaggagc ugaagaagua ccgcuggaac     1560

uacguguacg acgccgacaa ggacgagucc aagagcuacc ccaugaacga gacccgguac     1620

gagcugcgca agcggcucga caagguggag aacgucccgg ccgacuucau gacgcgcaag     1680

aucgagcagc accuguggca caucaucuac uccgugaccg acaagaucga guucgagaag     1740

gcccucaaga gcuucgcgaa caaguacgac cugcaccagg aguccuucgu ggagaacuuc     1800

aagcgguuca agcccuucga cagcgacuac gggaccuacu ccgagaaggc caucaagaag     1860

cugcugcccc ucaugcgcgu cggcaaguac uggagcuggg agggcaucga caacaacacc     1920

aagaagcgga ucgggaagau caucacgggc gaguacgacg agaccaucaa gaaccgcgug     1980

cgggagaagg ccaucgaccu gaccgaggag aaccacuucc aggggcugca gcucuggcug     2040

gcccaguaca ucgucuacga ccgccacucc gaggccgaca ucgcgggcaa guggaacagc     2100

guggccgacc uggagcagua ccucuccgag uucaagcagc acagccugcg gaaccccauc     2160

guggagcagg ucaucaccga gacgcugcgc guggugcgcg acaucuggaa gcaguacggg     2220

aagggcgcca aggacuacuu cgacgagauc cacaucgagc ucggccggga gaugaagaac     2280

accgccgagg agcgcaagcg gcuguccagc aucaucuccg agaacgagac caccaaccug     2340

cgcauccggg cgauccucga ggagcugaug uacgacgaga acauggagaa cguccgcccg     2400

uacagcccca ugcagcagga ggcccugaag aucuacgagg acggggugcu cagcuccagc     2460

guggagaucc ccgaggacau cgagaagauc uccaagaagu ccgagcccac caagagcgag     2520

auccagcggu acaagcugug gcuggagcag aaguaccgca gcccguacac gggccggccc     2580

aucccccucu ccaagcuguu cacccccgcc uaccagaucg agcacaucau cccgcaggcc     2640

cgcuucuucg acgacggguu cagcaacaag gucaucugcg aguccgaggu gaacgcccug     2700

aaggacaaga ugcucggccu ggaguucauc cagaacuacu acgggcagaa ggugaccacg     2760

agcuucggcg aggucaccau ccugaccgag gacgcguaca aggccuucgu gaaggagcac     2820

uacgacaaga accgguccaa gcgcaacaag cuccugcugg aggagauccc cgagaagaug     2880

aucgaccggc agaugaacga cacccgcuac aucagcaagu acaucuccaa ggugcucagc     2940

aaccuggucc ggggcgagaa ggacgacgag ggggugaacu ccaagaacau cauccccggc     3000

aacgggaaga ucagcggcac ccugaagcag gacuggggcc ucaacgacgu guggaacgac     3060

cugauccugc cgcgcuucga gcggaugaac cagcucacga acuccaccga cuucaccgcc     3120

uggaacgaga agcaccagaa guuccugccc accauccccc uggagcucag caagggguuc     3180

cagaagaagc gcaucgacca ccggcaccac gcccuggacg cccugaucau cgcgugcgcc     3240

acgcgcgacc acgucaaccu ccugaacaac aagcacgcca agagccggaa cgagcgcuac     3300

gaccugcagc acaagcuccg ccacaccacc cccuggcagg acaaggaggg caaggugcgg     3360

accaaguuca cggaguucaa gaagccgugg gagaccuuca cccaggacac caagaaggag     3420

cuggagaaga ucguggucuc cuucaagcag aaccugcgcg ugaucaacaa ggccacgaac     3480

uacuacgaga agaucgugga cgggaagaag gucauguuca agcaggaggg cgugaacugg     3540

gccauccgga agcccaugca caaggagacc gugagcggga agaucgaccu cccccgcguc     3600

aaggugucca agggcaagau ccugaccgcg acccgcaaga gccuggacac guccuucaac     3660

gccaagacca uccucagcau caccgacacc ggcauccaga agauccugcu gaacuaccuc     3720

aaggccaagg ggaaccccga gcuggccuuc uccccggagg gcaucgagga gaugaaccag     3780

aacaucagcc uguacaacga cgggaagccc caccagccca uccucaaggu gcgggucuuc     3840

gagcagggcu ccaaguuccc ccugggggag acgggcaaca agaccaccaa guacguggag     3900

gcggccaagg gcaccaaccu guucuucggg guguacgaga acaaccaggg caagcggagc     3960

uacaacacga ucccgcucaa ccuggucauc gagcgccaga agcaggggcu gaacuccgug     4020

cccgagacca acgagaaggg ccaccggcuc cuguucagcc ugucccccaa cgaccucgug     4080

uacgucccca ucgaggggga gguguucgac aagaccaacc cggacgugaa ccgcaucuac     4140

aagaucguca gcuucuccgg cagccagaug uucugcgugc ggcaggacgu ggccaccucc     4200

aucgucuaca agcuggaguu cagcucccug aacaagaugg agcgcacgau cgacggcauc     4260

augaucaagg agaucugcau caagcucaag guggaccggc uggggaacau cagcaaggcc     4320

uga                                                                   4323


<210>  2212
<211>  4323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2212
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc ccugguggag       60

caggacuucg agaacaagca ggggcucauc cugggcaugg ggucccgcau cauccccaug      120

gacgccggca ucaucggcaa guucgcggag gggagcucca ucagccagac ggccgagcgg      180

accggcuacc gcuccauccg gcgccugcgg gagcgccacc uccugcggcg cgagcggcug      240

caccgcgucc ucaacauccu gaaguuccug ccggagcacu acgccgccga gaucgacuuc      300

gagaagcggu ucgggcaguu ccucgccgag accgagccca agcuggcgug gaagaagaac      360

gccgagggca aguucgaguu ccuguuccag cgcagcuucg acgagauggu ggccgacuuc      420

aaggccaacg ggcaggacau caagaucccc uacgacugga ccaucuacua ccuccggaag      480

aaggcgcuga cgcagaagau cagcccccag gagcuggccu ggaucauccu caacuucaac      540

cagaagcgcg gcuacuacca gcugcggggc gaggaggagg aggagaaccc gaacaagaag      600

guggaguucu acagccugaa gaucgucgac gugguggccg acgagcagcc caacaagaag      660

ggggacaccu gguacucccu ccaccuggag aacggcuggg ucuaccgccg gagcuccaag      720

aucccccugu acgacuggaa ggacaaggug cgcgacuuca ucgugaccac cgacaucaac      780

gaggacggga gcgagaagcu cgacaaggac ggcgagguca agcgguccuu ccgcgccccc      840

aaggaggacg acuggacgcu gaucaagaag aagaccgagc aggagaucga gcaguucaac      900

cagaccgugg gcagcuacau cuacgaggcc cugcucgaca acccgaccca gaagauccgg      960

gggaagcugg ugcgcacgau cgagcgcaag uucuacaagu ccgagcugaa gaagauccuc     1020

gagaagcaga ucgagcugca gcccgagcug uucaccgagg accucuacaa cgcgugcgcc     1080

cgggagcugu accgccggaa cgucgcccac cagcagcagc ugagcucccg cgacuucgug     1140

uaccucuucc ugaacgacau caucuucuac cagcggcccc ugcgcagcca gaagucccuc     1200

aucagcaacu gcagccugga guuccggacc aucaaggaca aggacggcaa cgggcagauc     1260

aucuaccuga aggccauccc caaguccaac ccguacuacc aggaguuccg cguguggcag     1320

uggcucuaca accugaagau cuucaccaag gaggacgaca aggacgucac gaaccaguuc     1380

aucaacaagg uggaggaccu ggaggcccuc uucgaguucc ugaugaacca gaaggaggug     1440

aaccacaccg acauccugac cuaccucauc gagcccaucg ucaagagcaa guaccccaac     1500

gcgaagggca aggccuucaa ggacgagcug aagaaggagc ugaagaagua ccgguggaac     1560

uacguguacg acgccgacaa ggacgagucc aagagcuacc ccaugaacga gacccgcuac     1620

gagcugcgga agcgccucga caagguggag aacgucccgg ccgacuucau gacgcggaag     1680

aucgagcagc accuguggca caucaucuac uccgugaccg acaagaucga guucgagaag     1740

gcccucaaga gcuucgcgaa caaguacgac cuccaccagg aguccuucgu ggagaacuuc     1800

aagcgcuuca agcccuucga cagcgacuac gggaccuacu ccgagaaggc caucaagaag     1860

cugcugcccc ucaugcgggu cggcaaguac uggagcuggg acaucaucga cgacaagacc     1920

aagacgcgca ucggcaagau caucaccggg gaguacgacg agaccaucaa gaaccgggug     1980

cgcgagaagg ccaucgaccu gaccgaggag aaccacuucc agggccugca gcucuggcug     2040

gcccaguaca ucgucuacga ccggcacucc gaggccgaca ucgcggggaa guggaacagc     2100

guggccgacc uggagcagua ccucuccgag uucaagcagc acagccugcg caaccccauc     2160

guggagcagg ucaucacgga gacccugcgc guggugcggg acaucuggaa gcaguacggc     2220

aagggggcca aggacuacuu cgacgagauc cacaucgagc ucggccgcga gaugaagaac     2280

accgccgagg agcggaagcg ccuguccagc aucaucuccg agaacgagac cacgaaccug     2340

cggauccgcg cgauccucga ggagcugaug uacgacgaga acauggagaa cguccggccg     2400

uacagcccca ugcagcagga ggcccugaag aucuacgagg acggcgugcu cuccagcagc     2460

guggagaucc ccgaggacau cgagaagauc uccaagaagu ccgagcccac caaguccgag     2520

auccagcgcu acaagcugug gcuggagcag aaguaccgga gcccguacac cgggcgcccc     2580

aucccccucu ccaagcuguu cacccccgcc uaccagaucg agcacaucau cccgcaggcc     2640

cgguucuucg acgacggcuu cagcaacaag gucaucugcg aguccgaggu gaacgcccug     2700

aaggacaaga ugcucgggcu ggaguucauc cagaacuacu acggccagaa ggugacgacc     2760

agcuucgggg aggucaccau ccugaccgag gacgcguaca aggccuucgu gaaggagcac     2820

uacgacaaga accgcuccaa gcggaacaag cuccugcugg aggagauccc cgagaagaug     2880

aucgaccgcc agaugaacga cacccgguac aucagcaagu acaucuccaa ggugcucagc     2940

aaccuggucc gcggcgagaa ggacgacgag ggcgugaacu ccaagaacau cauccccggg     3000

aacggcaaga ucacggggau ccugaagcag gacuggggcc ucaacgacgu guggaacgac     3060

cugauccugc cgcgguucga gcgcaugaac cagcucacca acagcaccga cuucaccgcc     3120

uggaacgaga agcaccagaa guuccugccc uccguccccc uggagcucag caagggcuuc     3180

cagaagaagc ggaucgacca ccgccaccac gcccuggacg cccugaucau cgcgugcgcc     3240

acgcgggacc acgugaaccu ccugaacaac aagcacgcca agucccgcaa cgagcgcuac     3300

gaccugcagc acaagcuccg gcacaccacc cccuggcagg acaaggaggg gaaggugcgc     3360

accaaguuca cggaguucaa gaagccgugg gagaccuuca cccaggacac caagaaggag     3420

cuggagaaga ucgucgugaa cuucaagcag aaccugcggg ugaucaacaa ggccacgaac     3480

uacuacgaga agaucgucga cggcaagaag gugauguuca agcaggaggg ggugaacugg     3540

gccauccgca agcccaugca caaggagacc gucagcggca agaucgaccu cccccgggug     3600

aaggugagca aggggaagau ccugaccgcg acccgcaagu cccuggacac gagcuucaac     3660

gccaagacca uccucuccau caccgacacc ggcauccaga agauccugcu gaacuaccuc     3720

aaggccaagg gcaaccccga gcuggccuuc agcccggagg ggaucgagga gaugaaccag     3780

aacaucuccc uguacaacga cggcaagccc caccagccca uccucaaggu ccggguguuc     3840

gagcagggga gcaaguuccc ccugggcgag acggggaaca agaccaccaa guacguggag     3900

gcggccaagg gcaccaaccu guucuucggc gucuacgagg acaaccaggg gaagcgcucc     3960

uacgacacga ucccgcucaa caucgugauc gagcggcaga agcagggccu gaacagcgug     4020

cccgagacca acgagaaggg gcaccgccug cuguucuccc ugagccccaa cgaccugguc     4080

uacgugccca ucgagggcga gguguucgac gagaccaacc cggacgucaa ccggaucuac     4140

aagguggugu ccuucagcgg gucccagaug uucugcgucc gccaggacgu ggccaccagc     4200

aucguguaca agcucgaguu cuccagccug aacaagaugg agcggacgau cgacggcauc     4260

augaucaagg agaucugcau caagcugaag gucgaccgcc ucggcaacau cuccaaggcc     4320

uga                                                                   4323


<210>  2213
<211>  4332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2213
augaucaagc ugcucuaccu ggagaagcug aagcaguaca ccaugaagaa gauccucggc       60

cuggaccugg ggaccagcuc caucggcugg gcccucguga acgaggccga gaacagcgac      120

gaggucucca gcaucaucaa gcugggggug cgcgugaacc cccugacggu cgacgagaug      180

cagaacuucg agaagggcaa guccaucacc accaacgcgg accggacccu caagcgcagc      240

augcggcgca accugcagcg guacaagcug cgccgggacg cccucaucga gcugcugaag      300

aagguggggu ucaucacgga caagaccauc cucuccgagc agggcaaccg caccaccuuc      360

gagacguacc ggcugcgcgc caaggccgcc gaggaggaga ucagccugaa ggagcucgcg      420

cgggugcugc ugaugaucaa caagaagcgc ggcuacaagu ccagccggaa ggccaagggg      480

gaggaggagg gcgcccucau cgacgggaug gagaucgcca agaagcugua cgacgaggac      540

cugaccccgg gccagcucug ccugcagcug cucgaguccg ggaagaaguu cuuccccgac      600

uucuaccgca gcgaccugca ggaggaguuc aacaagaucu gggacuucca gaagcaguuc      660

aaccuguucu ccuucugcga cgucgcgaag gaggagguga agggcaagaa caagagccag      720

accugggcca uccucgccaa guacuucgug gagaccaaga aggucaacgu guggaacgag      780

cacgaggccc agacggagaa ccgggaggag guguacaagc uggucggccu gaagcgcacc      840

gugaaggggg aggagcucaa gaaggagaac uaccgguggc gcgugcaggc ccugaccgag      900

cagcuggacc ucgagaagau cgcggucgug cugcaggaga ucaacggcca gaucuccgcc      960

agcuccgggu accugggcgc caucagcgac cgguccaagg ugcucuacuu caaccgccag     1020

accgucggcc aguaccagau ggccgagcug aacaagaacc ccaacaugag ccugcggaac     1080

augguguucu accgccagga cuaccucgac gaguucaacg ugaucuggga gaagcaggcc     1140

gaguuccaca aggagcugac gcccgagcug aagaaggaga uccgcgacau caucaucuuc     1200

uaccagcggc gccucaagag ccagaagggg cugaucuccu ucugcgaguu cgagaaccgg     1260

gaggucgugg uggagaagga gggcaagaag cagaccaaga ucaucgggug caaggucauc     1320

ccgcgcagcc acccccuguu ccaggaguuc aagaucuggc agacccucaa caacaucgag     1380

guguucgucg gcgagaagaa ggggaagcgg aagaagucca acgugagcuc cgaccuguuc     1440

ggcgacaccg aggacgcgcu gaucacggag ggccgccggu uccuguacca ggaggagaag     1500

aagauccucg ccaaggagcu gagcaucaag aaggggcuga ccaaggccga gauccuccgc     1560

cugcuguucg agaaccccca ggagcucgac aucaacuuca agcagaucga cggcaaccag     1620

accggguucg cccuguucuc cgccuacagc aacaugaucg agaaguacgg cuacgagccc     1680

cuggacuuca agaagccggc ggaggaccuc aucucccagc ugaagaccgu guucacggac     1740

cuggggugga acaccgaccu ccuguucauc gaccuggaca aggagaacaa ggagugggag     1800

aagcagcccu acuuccggcu cuggcaccuc cuguacagcu ucgagggcga caacaccccc     1860

accggcaacg ggaagcucau cgagaagauc augcagcugu gcggcaucga gaaggaguac     1920

gccaucgagc uggccaacgu cucccuccag gacgacuacg ggagccuguc cgccaaggcc     1980

aucaagaaga uccugcucua ccugaaggag ggcaaccagu acgacauggc gugcgaguac     2040

gccggguacc gccacagcaa guccagccug acgaaggagg agaucgagaa caaggugcuc     2100

aaggacaagc uggagauccu gcccaagaac ucccuccgga acccgguggu cgagaagauc     2160

cugaaccaga uggugaacgu gaucaacacc gucauggagg uguacggccg ccccgacgag     2220

auccgggugg agcuggcccg cgagcucaag aaguccgcca aggagcggga ggagcugacc     2280

aaggcggucg ccaagaacac ccgcgagcac gaggagaucc ggaaggugcu gcagagcgag     2340

uucggcauga ucaacgugag ccgcaacgac aucauccggu acaagcucua cgaggagcug     2400

aaggacaacg gguacaagac gcuguacagc aaccaguaca ucccccgcga gaagcuguuc     2460

uccaaggaga ucgacaucga gcacaucauc ccccagagcc gccuguucga cgacucccug     2520

agcaacaaga cccucgagua caaguccauc aacaucgaga agggcaacaa gaccgccuac     2580

gacuucguca aggagaagua cggggaggag ggccugcagc aguaccugaa ccggugcgag     2640

aaccucugca aggacaagaa ggccaagcug cgcaagcuga agauggagcu caacgagauc     2700

ccggacggcu ucaucgaccg ggaccugcgc aacacccagu acaucgccaa gaaggcgcug     2760

agcaugcuca cggagaucug ccggcgcgug guggccacca ccggguccau caccgacaag     2820

cugcgggagg acuggcagcu ggucgacgug augaaggagc ucaaccugcc caaguacgag     2880

gcccugggcc ucguggaggu cuacgaggac aaggacgggc gcaagaucaa gcggaucaag     2940

gacuggacca agcgcaacga ccaccggcac cacgccaugg acgcccugac gguggcguuc     3000

accaaggacg uguucaucca guacuucaac aacaagaacg ccgccuggga gagcgacucc     3060

aaggagcaca ccaacaucgu cggcaucaag acccgcuacu ucgagagcgg gaaggccgug     3120

ccgcccaucc cccugggcca guuccgggug gaggccaagu gccaccucga gaaccugcug     3180

gucuccauca aggcgaagaa caagguggug acgaccaacg ucaacugcau caagaagaag     3240

ggcgggaccu acaagaacac ccagcagacg ccccgcggcc agcuccaccu ggagaccauc     3300

uacgggagcc acaaccagua cgugaccaag aucgagaagg ugaacgccuc cuucgacgcc     3360

gucaagaucg ccaccgugag caagcaggac uaccggaacg cgcugcucaa gcgccuggag     3420

guguucggca acgaccccaa gaaggccuuc acggggaaga acucccugga gaagaacccc     3480

aucuaccucg accgggccca gaccauccag gucccggaca aggugcagac cguggagcug     3540

gagaccgccu acacgauccg caagcccguc gaccccaccc ugaacgugga caaggugguc     3600

gacaagaaga uccggcucau ccuggagcag cgccugaagg aguacggcgg cgacgccaag     3660

aaggcguucg ugaaccucga cgagaacccc aucuggcuga acgaggagaa ggggaucagc     3720

aucaagcggg ugaccauccg cggcaucucc aaggcccagg cccugcacga caagaaggac     3780

aaggacggga acuucauccu cgacgagacc ggcaagcgca ucccggucga cuucgugaac     3840

acggggaaca accaccacgu ggccaucuac cggaagcccg uccuggacaa gaagggccag     3900

gugaucuucg acgacgaggg caaccccaug uacgagcugg acgaggacgu ggucccguuc     3960

uucgaggccg ugacccgcgc gaaccucggg auccccauca ucgacaagga cuacaagaag     4020

uccgagggcu ggcaguuccu guucagcaug aagcagaacg aguacuucgu cuuccccaac     4080

gagaagaccg gguucaaccc caaggaggug gaccugcucg acccggacaa cuacgccaug     4140

aucagcccca accuguuccg ggugcagaag aucggccuga acgacuacac cuuccgccac     4200

caccucgaga cgaccaucca gaacgaccug aaguucauca ccuggauccg gugcggcaag     4260

agcgggauca aggacaucgu caaggugcgc aucaaccaca ucggccagau cguguccguc     4320

ggggaguacu ga                                                         4332


<210>  2214
<211>  4332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2214
augaagaaga uccugggccu cgaccugggg accgccagca ucggcuccgc cuacgugcug       60

gaggcggaga aguccgccga ccagaccaag aucgucuacc ucgggacgcg caucauccug      120

uccagcgccg acgccgagaa cuucuccaag ggccugagcu gcucccccac caccgaccgg      180

accuucaagc gcagcaugcg gcgccggcuc cagcgcuaca agcagcggcg cuccagccug      240

auccagcagu ucaagaagau cgggaucauc acgaacaccg agcagaucau ccgggagccg      300

aucuccgaga cccacgagcu gcuccgcauc cggagcaagg ccguguccga gcacgugagc      360

cuggaggagu ucgcgcgcau ccugcucacc aucaacaagc ggcgcggcua caaguccaac      420

cggaagacga gcaaguccac cgaggaggac ggcaagcugg ucgacgccau ggccgugacc      480

aaggagcuga ucaagcucaa gaagaccccc gggcaguaca uguucgagcg cuucaagcgg      540

ggcaaguacg ccgagaucga cuucuacccc uucgaccuga ucaacgagcu gaagcagauc      600

ugggaggugc aggggcagaa guaccccgac guccucacgg agggccugcu gagcucccuc      660

cugaagcaga agaccgggaa ggcgaaggag cugaucuaca gcaagagcga ccucaacccg      720

gccgaggugc ccaucgacua ccagaagggc aagaagcugc gcaagaccau ccugaacaag      780

uacuucgccu acgagucccg gagccucgcc gugaccaaca ucauccccgu cgagcaggcc      840

aucacggcga ucguggccau cugcaagcag aucaacaacu ccagcggcua ccugggggcc      900

aucggcgacc gcucccgggu gcuguacgcc aacaaccaga ccaucgggca guaccucuac      960

gagcgcauca aggccaaccc ccacgaccgc auccggggcg aggucuucua ccgccaggac     1020

uaccuggacg aguucgaccg gaucugggag ugccagcaga acuacuaccc ggcgauccug     1080

accaacgacc gcaagcagua cauccggaac cgcaucaucu ucuaccagcg gccccucaag     1140

agcaagaagu ccgagcuggc cuucugcgag uucgagagcc gcgaggugga gcacaacggc     1200

aagaaguaca ccgaggggcu gcacguggcc cccaagucca gccccgucuu ccagcacuuc     1260

cggcucuggc agcgccuggg ccagaucgag guguccccgc ugcggaagug ggacaagcag     1320

agcaacgagc uguucgagca gcagaacaac gccgaccgcg aggggauccu caacacggag     1380

gccaagcaga agcuguucuc cuaccucaac cugcuggacc accucaccga caacgagauc     1440

cugaacaucc ugggcuacag ccccgacaag uggaagauca acuuccggga gaucaucggg     1500

aaccgcacca acaaccucau gaugaaggcg uucuucucca ugaaggagau gaccgacggc     1560

gagcagaucg acugggagaa gaaccugacg aagaucacca acggccagag ccugaucucc     1620

cccgagcaga ucguggaccu gauccggcag uucuucgagc gcgaggggau caccaccagc     1680

cuccuguacu ucgaccccgc cacggacaac uuccgggagc agucccacua cagccugugg     1740

caccuccugu acagcuacga ggacgacaac uccccgaccg gcaccaccag ccugugcaag     1800

acgcucgcca agcgcuacgg gaucccccac gagcugugcc cccugcucgc cucccugacc     1860

uucgagcggc aguacggcag ccuguccacc aaggccaugc gcaagcuccu gcccuucaug     1920

caggagaagc gcuacgacga ggcgugcgag cuggccgggu accggcacag cgccuggauc     1980

accaaggagg agaacgccaa gcgcacgcuc aagaacaagc ugccgcugcu ccggaagggc     2040

ucccugcgca accccgucgu ggagaagauc cugaaccaga ugacccacgu gaucaacacc     2100

cacaucgcgg aguacggcaa ccccgacgag auccggaucg agauggcccg cgacaugcag     2160

gccaacagca agcagcggaa ggccaugacc gccgcgauca ccgaggccac gaagcgcaac     2220

acccaguuca ucgaggagau ccagaaggag uucggguuca accccuccaa gaacgaccuc     2280

aucaaguaca agcuguggaa ggagcuggcc ccgcucggca acaagacccu guacagcaac     2340

aucuacaucc ccaaggagga gcuguucacc gccaaguucg agaucgagca caucaucccc     2400

cgggcccucu ucuacgacga cuccuucagc aacaagacgc uggagcugaa cuccgucaac     2460

aagcucaagg ggaacaagac cgcgcuggac uaccuggaca cccucggcca ggagaaggcc     2520

aacgacuaca uccugcgcgu ggaggagcug aagaagaucg gggccaucac ccaggccaag     2580

uacaagaagc uccugauccg ggagaaggac cugggcgacg acuucacgaa ccgcgaccuc     2640

accaacaccc gguacaucau ccgccacgcc gcggaccggg accugaccaa cacgcgcuac     2700

aucauccggc acgccgccga caugcugcuc caggugaccc ccagcaucgu ccacaccaac     2760

ggcuccauca ccgccauccu gcgccgggac ugggggcugc ucgacauccu gaaggagcug     2820

aaccucccga cguacgagag caucgaccgc aagaucuccg accccgagga gaagcugacc     2880

cagagcgugg accggaagga cggccaggcg aucaagauga ucaaggacug gagcaagcgc     2940

gacgaccacc gccaccacgc cauggacgcc cugaucaccg cccucaccac gcggaagcac     3000

guccaguacc ugaacgggaa cuccggcgcc gaggagaccg acaaggcgca gaagcuggag     3060

cagcucaagg cccugcgcaa gcagcugauc ggcagcgacg ggcacuucau cccgcccaug     3120

cacgacaucc gggugcaggc caaggaggcc cucucccgcg ugaucaucag caccaagguc     3180

aagggcaagg ugaccacgcc caacaccaac aucaucugcu cccgggacaa gaccaccggg     3240

cgcaagaucu acgugcggca gacgacccug acgccccgcg gcgcccugca ccucgacacc     3300

aaguacggga ucaaccggua cuaccaggag cgcgagguca gcaucggcuc caagcugacc     3360

caggaggaga uccggcuggu gagcagcccc cacauccaga gcauccuccu ggagcgccug     3420

cgggcguucg acaacaaccc gaaguccgcc uucaccggcc gcaacagcgu ggacaagaag     3480

ccccucgagg acaagaacaa gaagcugguc uacaccgaca agaaggugaa gcagaccauc     3540

cuggccaacc gguucgugaa gcgcgaccgg gucaacucca agcucgaccu ggacaacgug     3600

aucgacacgg ggaucaagcg cauccuggag cggcgccuca agcaguacgg cgggaaguac     3660

gagaccgccu ucagcgaccu ggagaacaac cccaucuggc ugaacgagga gaagggcauc     3720

gccaucaagu ccgugcgcau ccgggggaag aacaacacca ucaccauccg ccagaccaac     3780

acgugccgga ucaaguacgu cgcgaacgac aacaaccacc accucgccgu guacgcccug     3840

cccgacggca gcaucaagga cgacaucgug uccuucuucg acgccgucga gcgcagccgg     3900

aagcgccugc cgaucaucga caagcacaag aaccaguccc ucggcuggaa guaccuguuc     3960

agcaucaagc agaacgagua cuuccugcuc ccccagcagg ucgagaagau cgacaucgac     4020

accggggaga ucagcaugca gaccaccuuc ucccccaagg accugagcca cgaguacauc     4080

augaaccccg acaacuacgc ggagcuggcc ucccacaucu uccgggugca gagcaucucc     4140

aagacgccgc agggcaaccg cgacgugcgg aucuaccucu ucaagcagca guacaucagc     4200

gggucccaga aggacagccu gcaccugaag ggcaucaccc accgccagau cuccagcgcc     4260

aaggaccucg ccgagcuggu gaagauccgg gugaaccacc uggggcgcau cgucuccgug     4320

ggcgagaagu ga                                                         4332


<210>  2215
<211>  4341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2215
auggugaaga acauccuggg ccucgaccug gggaccaaca gcgucggcug ggcccugauc       60

aagcaggacu ucgagaacaa gcagggggac auccucggca uggggucccg caucaucccc      120

augagccagg acgugcuggg cgacuucggc aaggggaacu ccaucagcca gaccgccgag      180

cggacgaagu accgcuccgu gcggcgccug cgggagcgcu uccuccugcg gcgcgagcgg      240

cugcaccgcg uccucaacgu gcuggacuuc cugccggagc acuacgcgag ccagaucgac      300

uucaacaagc gguucggcaa guucaaggag gagaccgagc ccaagcucgc cuacuccaac      360

gacggguuca ucuucaagaa guccuucgag gagaugauca gcgacuucaa gacccaccag      420

cccgagcugc uggagaacaa caagaaggug cccuacgacu ggaccaucua cuaccuccgc      480

aacaaggccc ugagccacaa gaucgagaag gaggagcugg ccuggauccu ccugaacuuc      540

aaccagaagc ggggcuacua ccagcugcgc ggggaggagg aggaggagaa cccgaacaag      600

cucaucgagu ucuacucccu gaagaucguc gacgugcugg ccgacgagcc ccagaagggc      660

aagagcgaca ucugguacuc ccucauccug gagaacggcu ggaucuaccg gcgcagcucc      720

aagauccccc ugcucgacug gaaggggaag acgcgggacu ucaucgugac caccgaccug      780

aacgacgacg gcagcgucaa gaccgacaag gaggggaacg agaagcgcuc cuuccgggcg      840

cccggcgaga acgacuggac gcuggugaag aagaagaccg agcaggagau cgacaacagc      900

gacaagaccg ugggcgccua caucuacgag gcccuccugc agaacccgaa gcagaagauc      960

aaggggaagc ugguccgcac caucgagcgg aaguucuaca aggccgagcu caagcagauc     1020

cuggagaagc agauggaguu ccaccccgag cugcgccagg acgaccucua cuccgacugc     1080

gugcgcgagc uguacaagaa caacgaggcg caccagcuga cgcucagcaa gaaggacuuc     1140

gugcaccugu ucauggagga caucaucuuc uaccagcggc cccugcgcag ccagaagucc     1200

agcaucucca acugcacccu cgaguuccgg aaguacaagg acgagaacgg caccgagcac     1260

auccaguacc ugaaggcccu gcccaagagc aacccguacu accaggaguu ccgcaucugg     1320

caguggaugu acaaccucaa caucuacaag aaggaggacg acaccaacgu cacgaaggag     1380

uuccugaacu ccaaggccga cuucgagagc cuguucgagu uccucaacaa caagaaggag     1440

guggaccaga aggcccugcu gaagcacuuc aagaucaacg acaagaccca ccgguggaag     1500

uacguggagg acaagaagua ccccugcaac gagaccaaga ccaugaucuc cgagcgccug     1560

aagaaggucg agaacaucag cgacgacuuc cucacgcggg agauggagca gaagaucugg     1620

cacaucaucu acuccgugaa cgacaaggug aaguacgaga aggcccucaa gagcuucgcg     1680

gagaagaaca accuggacga guccagcuuc uucgaggccu ucaagaaguu cccgcccuuc     1740

aaguccgagu acggggccuu cagcgagaag gccaucaaga agcuccugcc ccugaugcgc     1800

cucggcaagu acugggacga cgacgagauc cugaaguacu ccgacaucua cuucaagaac     1860

aucgaguacc ugcucggggu ccugaagcag aaggacaaga acaucagcga cgacaaccgg     1920

cagaacugga acaaguccau caacaacaag cugcgcgagg agcucgagau cuuccagaac     1980

gccgagaucg agagcuucca gggccugcgg cugcacaucg cgcaguacau ccucuacggc     2040

cgccacuccg aggccgccau gcuggggaag uggaacagcg ccgacgaccu ggaggaguuc     2100

cucaaggagu ucaagcagca cagccugcgg aaccccaucg uggagcaggu caucaccgag     2160

acccugcgcg uggugaagga caucuggguc aaguacggca acggggccaa ggacuucuuc     2220

aacgagaucc acaucgagcu cggccgggag augaagcugc ccgcggagga ccgcaagaag     2280

aucaccaacc agaucuccga gaacgagaac acgaaccugc ggaucaaggc ccuccuggcc     2340

gagaugaugg uggacagcuc cguggagaac guccgccccu ucagcccgau gcagcaggag     2400

auccugaaga ucuacgagga cggggugcuc aacuccgaca ucgagaucga ggacgacauc     2460

cugaagauca gcaagaccgc ccagcccucc cccagcgacc ugaagcggua caagcucugg     2520

cuggagcaga aguacaaguc ccccuacacc ggccagauca ucccgcugaa caagcuguuc     2580

acccccgagu acgagaucga gcacaucauc ccccagagcc gcuacuucga cgacuccuuc     2640

agcaacaagg ugaucugcga ggccgcgguc aacaagcuga aggacaacuu caucggccug     2700

ggguucauca agcaguucgg cgggacgauc guggagugcg gcaacgggaa guccgugacc     2760

guccucaagg aggaccagua cgaggacuuc gugaagaagc acuacgccaa caaccgcagc     2820

aagcggaaca agcugcugcu cgaggacauc cccgagaaga ugaucgagcg ccagaugaac     2880

gacacccggu acaucuccaa guacaucagc ggcgugcugu ccaacaucgu ccgcaccgag     2940

gacggcacgg acgacggggu gaacagcaag aacaucgugc cgggcaacgg gaagaucacc     3000

aacacccuga agcaggacug gggccucaac gacaucugga acgaccugau ccugccccgg     3060

uucgagcgca ugaaccagcu caccaacagc acggacuuca ccgccuggaa cgagaaccac     3120

cagaaguucc ugcccaccgu cccgguggag uucuccaagg gcuucagcaa gaagcggauc     3180

gaccaccgcc accacgcccu ggacgcgcuc gugaucgccu gcgccaccaa ggaccacguc     3240

aaccugcuga acaaccaguc cgccaagagc gacaccaagc ggcacgaccu caagcgcaag     3300

cugaugaagu ucgagaaggu ggccuacaac cacccccaga cgggggagcg gaucgagcgc     3360

gaggugccca agcaguuccu gaagcccugg gagaccuuca ccgucgaggc gaagaacggc     3420

cucgagacca ucaucguguc cuucaagcag aaccugcggg ugaucaacaa ggccacgaac     3480

cacuacgaga aguacgucga gaaggacggg gccaagguga agggccgcgu ggagcaggcc     3540

gggaccaacu gggccauccg gaagccgaug cacaaggaga ccgucagcgg caagaucgac     3600

cugccccgca ucaaggugcc caagggcaag auccucaccg cgacgcggaa gaaccuggac     3660

accuccuucg accugaagac caucgggagc aucaccgaca cgggcaucca gaagauccuc     3720

aagaacuacc ugcaguacaa ggacaacaac cccgagcugg ccuucucccc ggaggggauc     3780

gaggacauga acaagaacau cgagaaguuc aacgacggca aggugcacca gcccaucauc     3840

aagguccgca ucuucgagcu cgggagcaag uucccccugg gccaguccgg caacaagaag     3900

gacaaguacg uggaggccgc caaggggacc aaccuguucu ucgccgugua cgaggacaag     3960

aacggcaagc ggagcuacga gaccaucccc cucaacgagg ucaucgagcg ccagaagcag     4020

gggcugaccg ugguggaccu gaagggcguc aacgacuucu accucucccc gaacgaccug     4080

guguacgugc ccagcgagga cgagcuggag aacgucucca ugaucgacua caacaagcuc     4140

agcaacgaga agguguacaa gguggucucc uucacgggga accagaucuu cuucgugcgg     4200

caggacgugg cgaccagcau cgucaacaag gccgaguucu ccagccugaa caagauggag     4260

cgcgccaucg gcggcaccau gaucaaggag guguucguga agaucaagau cgaccgccug     4320

gggaacauca gcaaggucug a                                               4341


<210>  2216
<211>  4347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2216
augaagcaca uccugggccu cgaccugggg accaacagca ucggcugggc cguggucaag       60

gaggcccaga acgacaacga gcaguccgag aucguggacg uggggguccg cgugaacccc      120

cugaccacgg acgagaagac caacuucgag aagggcaagc cgaucagcac caacgcggac      180

cggacccuca agcgcuccgc ccggcgcaac cugcagcggu acaagcugcg ccggaaggag      240

cucaucgagc ugcugaagga gcaccagcuc aucagcgaga acacgauccu gaccgagaac      300

gggaagggca ccaccuucca gacgcuggag cuccgcgcca aguccgccaa ggagaagauc      360

agccugggcg agcuggcccg gguguuccuc accaucaaca agaagcgcgg guacaagucc      420

agccggaagg cgaagaacgg cgacgagggg cagcugaucg acggcaugga gaucgccaag      480

cgccuguacg aggagaaccu caccaccggg cagcuguccc ugcagcuccu gaaggagggc      540

aagaaguccc ugcccgacua cuaccggagc gaccuccagu ucgagcugga caaggucugg      600

aacuuccaga agcagagcca cuccgacauc cugacggacg aguucuacgc ccagcucaag      660

ggcaaggggc agcgcgccac cagcgcgcug uuccuggcca aguaccagau cuacaccgcc      720

gagaacaagg gcucccggga ggacaagaag cuccaggccu accaguggcg cguggacgcc      780

cugagcaagg agcugacccg ggaggagcuc gcguucguga ucacggagau caacaacaac      840

cugaacaacu ccagcgggua ccugggcgcc aucuccgacc gcagcaagga gcucuacuuc      900

aacaacgaga ccgucggcga guaccugugg aagcagaucg ccgccaaccc ccacaacagc      960

cugaagaacc agguguucua ccggcaggac uaccucgacg aguucgagca gaucuggaag     1020

acccaguccg ccuucuaccc cgagauccug accgacgagc ugaagaagga gcuccgcgac     1080

guggucaucu ucuaccagcg gaagcucaag agccagaagu cccuggugag cuucugcgag     1140

uucgagaaca aggagaucga gaucaagaac gaggacggga cgaccaccaa gaaggugauc     1200

ggccugaagg ucgcgccgaa guccagcccc cuguuccagg aguucaagau cuggcagaac     1260

cugaacaacg ugcucauccg caagaagggg uccaagacca agaagguggc caagaaccag     1320

caggccgucc uguucgagga ggagaagacg aucagcgagc uggaccugga ggccaagcag     1380

gcccucuuca acgagcugaa ccugaagggc aaccucaagg cggacuacug ccuggagauc     1440

cugggguacu cccccaagga gugggagaug aacuacagca uccucgaggg caaccgcacc     1500

aacaaggccc uguacgaggc cuaccugaag aucaccgacc ucgagggcua cgacgccaag     1560

aaccaccuga aggugaagcu gaacaaggac gagguggagc ucgacgaccu gaaggucccc     1620

gccuccgaga ucaaggacau ggugcaggcg aucuucaagg aggccgggau caacaccgac     1680

auccugcacu ucaacgccga gcucgacggc aaggacuucg agaaccaggc cagcuaccag     1740

cuguggcacc uccuguacuc cuacgagggg gacgacagca aguccggcaa cgagcugcug     1800

uacaagcucc uggagacgaa guucggguuc aagaaggagu acgcgcagau ccuggccaac     1860

gugagccucu ccgacgacua cggcaaccug agcaccaagg ccaugcggga gaucaucacc     1920

caccugaagc agggcuuccc guucaccggg cgcaaggacc ggcccgacga gcccuccgcc     1980

ugcgaguucg ccggcuacaa gcacagcaag cacagccuca cgaaggagga gaacgagaag     2040

cgcguccuga aggacucccu ggaggugcuc aagaagaaca gccugcggca gcccgucgug     2100

gagaagaucc ugaaccagau gaucaaccuc gugaacgcgc ugcugaagaa guaccgcacc     2160

gagaacccga acuucaaguu cgacgagauc cggaucgagc ucgcccgcga gcugaagaag     2220

aaggccgagg agcgggccaa caugaccgcc aacaucaaca aggcgaagac cgagcacgag     2280

aagaucauca agauccugaa gaacgaguuc gggcucccca accccacgcg caacgacauc     2340

auccgguacc gccuguacga ggagcugaag gacaacggcu acaaggaccu cuacaagggg     2400

gacuacaucc cccgggagaa gcuguucucc aaggagaucg acaucgagca caucaucccg     2460

aaggcccgcg ucuucgacga cagcuucucc aacaagacca ucaccuaccg gaagaccaac     2520

cuggagaagg gcgagcgcac cgccuacgac uacaucgaga gccucggcga gaagcagcug     2580

gaggaguucc ugucccggau cgagaaccuc uacgagaucg ggaagcccag ccgccugcac     2640

aaccggcgca acccccugaa gaagguggag gagaagggca ucuccgacag caaguacaag     2700

aagcuccuga agaaggaguc cgagaucggg gacggcuucg uggagcgcga ccugcgggag     2760

acgcaguaca ucgccaagaa ggccaaggag auccuccugc agaucacccg cagcguccug     2820

uccaccagcg gcgggaucac cgaccggcuc cgcgaggacu gggaccugau caacgugaug     2880

aaggagcuga accuccccaa guaccgggcg cugggccuga cgaagaugga ggaccgcaag     2940

uacgggcagc aggucgaggu gaucaccgac uggaccaagc ggaacgaccu ccgccaccac     3000

gccauggacg cccugaccgu ggccuucacg aagcacaacc acauccagua ccugaacuac     3060

cucaacgcgc ggaagaacga gaagcacaag gagcacaaca acaucauggc cauccagcag     3120

cuggagacca ucaaggugac cgacgagaac ggcaacgaga agcgcaucuu caaggccccg     3180

augcccaacu uccggcaggu cgccaaggcc uuccuggaga acgugcucau cucccacaag     3240

gcgaagaaca agguggucac caagaacaag aacaagauca gcgggaacaa caaggugcag     3300

gaggugcuga cgccccgcgg ccagcugcac aaggagacca ucuacggcaa guaccaguac     3360

uacguccaga aggaggagaa gaucgggggc aaguucgacc ucgagaccau cgagaaggug     3420

gccaaccccc uguaccaguc ccugcucaag aagcggcugg aggagaacgg gggcgacccg     3480

aagaaggccu ucaccgggaa gaacagccug gccaagaacc ccaucuaccu cgacgaggcc     3540

cagacggagc agcugcccga gaaggugaag cuggucuggc ucgagaacag cuucuccauc     3600

cgcaaggaca ucacccccga gaacuucaag gacgagaagc ugaucgacaa gauccuggac     3660

gagggcguga agcggauccu caaggagcgc cugcagagcu ucggcaacga cgcgaagaag     3720

gccuucuccg accuggagaa gaacccgauc uggcucaaca aggagaaggg gaucgccauc     3780

aagcggguga ccaucagcgg cgucgccaac gccgagcccc ugcacaccaa gaaggaccac     3840

cuggggaagg agauccucga caaggagggc aagcccaucc cgguggacuu caucuccacg     3900

gggagcaacc accacguggc ggucuaccgc gacgagaagg gcaaccugca ggagaaggug     3960

guguccgucu ucgaggccgu ggugcgggcc aaccagggcc ugcccaucau cgacaagacc     4020

uacaaccagu accucgggug ggaguuccug uucagcauga agcagaacga gauguucguc     4080

uuccccaacg aguccaccgg cuucaacccc aacgacaucg accugcugga cccgaagaac     4140

aagaagauca ucagccccaa ccucuuccgc gugcagaagc uggccaccgg ggacuacugg     4200

uuccggcacc accuggagac gaccguggag accaaguccg cgaccaagga caugacguac     4260

aagcgccuca gccugaacca caucaagaac aucaucaagg uccgcaucaa ccaccugggc     4320

gacaucgugu ccguggggga guacuga                                         4347


<210>  2217
<211>  4353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2217
augaagcaca uccugggccu cgaccugggg agcaacucca ucggcugggc cuucgugcag       60

caggacuucg agaacaagca ggggaagauc aucgccaccg gcagccgcau cauccccaug      120

gaccagggga uccugggcga cuucgagcgg ggcaacaccg ucucccagac ggcggagcgc      180

accaccuacc ggaacacccg ccggcuccgc gagcggcacc uccugcgccg ggagcgccuc      240

caccgggugc ugcacauccu gggguuccuc ccgccccacu acgacagcca gaucgacuuc      300

acgaagcgcu acggcaaguu caucgacaac gccgagccca agaucgccua caacaacggg      360

aacuucaucu ucaugaacuc cuucaacgag augaucgagg acuucaagaa guaccagccc      420

cagcuguucu acaagaaguc caacggcgag gaguccaaga ucccguacga cuggaccauc      480

uacuaccugc ggaagaaggc ccucagccag aagaucaccc agcaggagcu ggccuggcug      540

auccuccacu ucaaccagaa gcgcggguac uaccagcugc ggggcgagga ggagaccgag      600

aaccccaaca aggagguggc guuccacucc cuggaggucg uggacgugga ggccgaggcc      660

cccaacaaga agggcgagau cugguacacg auccgccucg agaacgggug gaucuaccgg      720

cgcaccagca agaucccccu ggacgacugg aagggcaaga cccgggacuu caucgucacc      780

acggaccuga acgacgacgg gaccaucaag cucgacaagg agggcaagga gaagcgcucc      840

uuccgggccc cggacgagaa cgacuggaac cugcugaaga agaagaccga gcacgacauc      900

gacuucagcg gcaagaccgu gggggcguac aucuacgacc accuccugca gaaccccaac      960

cagaagauca agggcaagcu ggugcgcacg aucgagcgca aguacuacaa ggacgagcuc     1020

aaggccaucc uggccaagca gaucgcccug cagcccgagc uguucaccga gacccugcug     1080

gccgacugca uccgggagcu cuaccgcaag aacgagaccc agcagcagaa ccugcugucc     1140

aaggacuucc uccaccuguu cgucgaggac aucaucuucu accagcggcc ccugcgcagc     1200

aagaagucca cgaucgcgaa cugcacccuc gagaagcgga gcuacaucga caaggagacc     1260

aacgagcgca aggacacccc gaucaaggug ugcgccaagu ccaaccccua cuaccaggag     1320

uuccgggugc ugcaguggcu gcagaaccuc cgcaucuacg agaucgagac ggaccgggag     1380

gucaccgccg aguucaucga gaccgcccag gacaacgagg cccuguucaa cuuccugaug     1440

agccagaagg agaucgacaa cgagucccuc cugaaguacu uccuguucgc gaagaacccc     1500

cagaucaagg acaagguggc caagagcgag cucaagaagu ggcugaccac guaccgcugg     1560

aacuacgugu acgacgccgc cgacaacagc uccaagaagu accccaugaa cgagaccggg     1620

uacgagcugc agcaguaccu gcagaagguc gagggccucc cggccaacuu ccugaccuuc     1680

gacaucaccu accggcugug gcaccuccug uacagcguga aggacaaggu ggaguucgag     1740

aaggcgcuga agaaguucgc ccaggaguac cagcucgacg aggccuccuu cgucgacagc     1800

uucaagaacu ucaagcccua ccccaacgag uacggguccu ucagcgagaa ggccaucaag     1860

aagcugcugc cccucaugcg cuucggcaac cacugggacu ucaaccggau cgacgugucc     1920

acgcagaagc gcaucgacga ccugaucaac ggcguggcca acgacgagau ccggaccauc     1980

auccgcgaga aggcggagaa guaccagcug aacagcgaga ccugcuucca ggggcucccg     2040

cuguggcugg cccaguacgu cguguacaac cggcacgccg aggccgcgga caucgagaag     2100

uggaccuccg ugaacgaccu cgaggccuac cuggaggacu ucaagcagca cagccugcgc     2160

aaccccaucg ucgagcaggu caucacggag acccuccggg uggugcgcga caucuggcag     2220

cgcuacggcg ggggccaggc caacuucuuc gacgagaucc acaucgagcu ggggcgggag     2280

cugaagaaga ccgccgagga gcgcaagcag cucuccaacc ggaaccagga gaacgagaac     2340

accaaccugc gcaucaagaa gcugcucgcc gagcugaagg aggacgcgag cauccagaac     2400

gugcggcccu acucccccgu ccagcaggag cugcucaaga ucuacgagga gggcguguuc     2460

agcgccaccg acgaggugga ggaggacauc cagaagaucc gcaagaaggc cgagccgacg     2520

acccccgaga uccagcggua caagcugugg cuggagcaga aguacaaguc ccccuacacc     2580

ggcgagguca ucagccucac caagcuguuc acggaggagu acgagaucga gcacaucauc     2640

ccccaguccc gcuacuacga cgacagccug uccaacaagg ugaucugcga gagcgccgug     2700

aacaagcuca agaccaacca gcuggggcug gaguucaucc agaaccacca cggcgagaag     2760

guccggauca ccgccaacaa ggaggugacc aucuucacgg aggagcagua caaggaguuc     2820

gugaagaagc acuacgcgaa caacagcgcc aagcagcaga agcuccugcu gaacgagauc     2880

ccggagaaga uggucgcccg ccagcucaac gacacccggu acaucuccaa guacaucagc     2940

gagauccugu ccaagaucgu gcgcagcgac gagaaggacg agggggugaa cuccaagaac     3000

aucauccccu gcaccggcaa gaucaccacg gcccugaagc aggacugggg gcucaacgac     3060

gucuggaacg agcugauccu gccccgguuc gagcgcauga accagcucac ccagacccag     3120

cuguucacca gcuacaacga ccgguuccag aagaaccugc cgacggugcc caucgaguac     3180

ucccagggcu uccagaagaa gcgcaucgac caccggcacc acgccaugga cgcgcucgug     3240

aucgccugcg ccacccgcga gcacaucaac uacaugaaca accagcacgc ccuggacaag     3300

agcaaggaca agaaggagaa gcagcagucc cggaucgacc ugcgcaccau ccucugcgag     3360

aagaagaaca acaacuggca guucaagaag cccuggagca ccuucacgca ggaggcgcag     3420

gaggcccugg agcacaucgu cguguccuuc aagcagaacc ugcgggugau caacaaggcc     3480

accaacuacu acgagaagug ggaggagaag aacggcgucc ucgugaagaa gaaggugaag     3540

caggagggga ccaacugggc cauccgcaag ccccugcaca aggagaccgu cagcggcaag     3600

aucgugcugg accgcaagau cgggaaggac aagauccuca cggccacccg gaaguccgug     3660

gacaucaccu ucaccgagaa gaucaucagc uccaucacgg acaccggcau ccagaagauc     3720

cugcugaacu accucaagua caagggcagc ccggaggucg cguucuccgc cgaggggcug     3780

gaggagcuga acaagaacau cgcccaguac aacgacggca agaagcacca gcccaucuac     3840

aaggugcgca ucuucgagga ggggagcaag uucccccucg gcgagaccgg ggccaaggcc     3900

accaaguacg uggaggcggc caagggcacg aaccuguucu ucggcgucua ccaggggaag     3960

ggcaagcgga ccuacgccac caucccccug aacgagguga ucgagcgcca gaagcagggg     4020

cucccgagcg ugcccgagcg gaacgagaag ggcgagcccc ugcuguucuc ccucagcccc     4080

aacgaccugg ucuacgugcc gauggagggg gagaucgccg agaccaucga cuucaacaac     4140

cuguccaagg agcagaagga gcgcaucuac aagacgguga gcuucaccgg caaccagugc     4200

uucuucgucc aggaggccgu ggcgaccccc aucgugaaca agaucgagua cagcccccuc     4260

aacaagaugg agcgggacau ccugggcacc augaucaagg aggucugcau caagcugaag     4320

guggaccgcc ucgggaacau caccaaggcc uga                                  4353


<210>  2218
<211>  4353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2218
augaagcgca uccugggccu cgaccugggg agcaacucca ucggcugggc cuucguggag       60

caggacuucg agaacaagca ggggaagauc aucgccaccg gcagccggau cauccccaug      120

gaccagggga uccugggcga cuucgagaag ggcaacaccg ucucccagac ggcggagcgc      180

accaccuacc ggagcaugcg ccggcuccgc gagcggcacc uccugcgccg ggagcgccuc      240

caccgggugc ugcacauccu gggguuccuc ccgccccacu acgacgccca gaucgacuuc      300

accaagcgcu acggcaaguu caucgacaac gccgagccca agaucgccua caacaacggg      360

aacuucaucu ucaugaacuc cuucaacgag augaucgagg acuucaagaa guaccagccc      420

cagcuguucu acaagaaguc caacggcgag gagagcaaga ucccguacga cuggacgauc      480

uacuaccugc ggaagaaggu gcucagccag aagaucaccc agcaggagcu ggccuggcug      540

auccuccacu ucaaccagaa gcgcggguac uaccagcugc ggggcgagga ggagaccgag      600

aaccccaaca aggaggucgc guuccacucc cugaaggugg uggacgucga ggccgaggcc      660

cccaacaaga agggcgagau cugguacacc auccgccucg agaacgggug gaucuaccgg      720

cgcacgagca agaucccccu ggacgacugg aagggcaaga cccgggacuu caucgugacc      780

accgaccuga acgacgacgg gacgaucaag guggacaagg agggcaagga gaagcgcucc      840

uuccgggccc cggacgagaa cgacuggaac cuccugaaga agaagaccga gcacgacauc      900

gacuucagcg gcaagaccgu cggggcguac aucuacgacc accuccucca gaaccccaac      960

cagaagauca agggcaagcu ggugcgcacc aucgagcgca aguacuacaa ggacgagcug     1020

aaggccaucc ucgccaagca gaucgcccug cagcccgagc uguucacgga gacccuccug     1080

gccgacugca uccgggagcu guaccgcaag aacgagaccc agcagcagaa ccuccugucc     1140

aaggacuucc ugcaccucuu cguggaggac aucaucuucu accagcggcc ccugcgcagc     1200

aagaagucca ccaucgcgaa cugcacgcug gagaagcgga gcuacaucga caaggagacc     1260

aacgagcgca aggacaccuu caucaagguc ugcgccaagu ccaacccgua cuaccaggag     1320

uuccgggugc uccaguggcu gcagaaccug cgcaucuacg agaucgagac cgaccgggag     1380

gugacggccg aguucaucga gaccgccgag gacuacgagg cccucuucaa cuuccugaug     1440

agccagaagg agaucgacaa cgaggggcug cucaaguacu uccuguucgc gaagaacccc     1500

cagaucaagg acaaggucgc caaguccgag cugaagaagu ggcucaccac cuaccgcugg     1560

aacuacgugu acgacgccgc cgacaacagc agcaagaagu accccaugaa cgagacgggc     1620

uacgaccugc agcgguaccu gcagaaggug gaggggcugc ccgccaacuu ccucaccuuc     1680

gacaucaccu accgccugug gcaccuccug uacuccguca aggacaaggu ggaguucgag     1740

aaggcgcuga agaaguucgc ccaggaguac cagcuggacg aggccagcuu cguggacucc     1800

uucaagaacu ucaagccgua ccccaacgag uacggcagcu ucuccgagaa ggccaucaag     1860

aagcuccugc cccugaugcg guucggcaac cacugggacu ucaaccgcau cgacgucagc     1920

acccagaagc ggaucgacga ccucaucaac gggguggcca acgacgagau ccgcacgauc     1980

auccgggaga aggcggagaa guaccagcug aacuccgaga gcugcuucca gggccugccc     2040

cucuggcugg cccaguacgu ggucuacaac cgccacgccg aggccaccga caucgagaag     2100

uggaccuccg ugagcgaccu ggagaccuac cucgaggacu ucaagcagca cucccugcgg     2160

aacccgaucg ucgagcaggu cgugacggag acccugcgcg uggugcgcga caucuggcag     2220

caguacgggg gcgggcaggc gaacuucuuc gacgagaucc acaucgagcu cggccgggag     2280

cugaagaaga ccgccgagga gcgcaagcag cugagcaacc ggaaccagga gaacgagaac     2340

accaaccucc gcaucaagaa gcugcuggcc gagcucaagg aggacgccuc cauccagaac     2400

gugcggcccu acagccccgu ccagcaggag cugcugaaga ucuacgagga gggcguguuc     2460

uccagcaccg accaggugga ggaggacauc cagaagaucc gcaagaaggc cgagcccacg     2520

accccggaga uccagcggua caagcucugg cuggagcaga aguacaaguc ccccuacacc     2580

ggggagguca ucagccugac caagcuguuc acggaggagu acgagaucga gcacaucauc     2640

ccccaguccc gcuacuacga cgacagccug agcaacaagg ugaucugcga guccgcggug     2700

aacaagcuga agaccaacca gcucggccug gaguucaucc agaaccacca cggggagaag     2760

guccggauca ccggcaacaa ggaggugacc aucuucacgg aggagcagua caaggaguuc     2820

gugaagaagc acuacgccaa caacagcgcc aagcagcaga agcugcuccu gaacgagauc     2880

cccgagaaga uggucgcccg ccagcugaac gacacccggu acaucuccaa guacaucagc     2940

gagauccucu ccaagaucgu gcgcagcgac gagaaggacg agggggugaa cuccaagaac     3000

gucaucccgu gcaccggcaa gaucaccacg gcccugaagc aggacugggg ccugaacgac     3060

guguggaacg agcucauccu gccccgguuc gagcgcauga accagcugac ccagacccag     3120

cuguucacca gcuacaacga ccgguuccag aagaaccugc ccacggugcc gaucgaguac     3180

ucccaggggu uccagaagaa gcgcaucgac caccggcacc acgcgaugga cgcccugguc     3240

aucgccugcg ccacccgcga gcacaucaac uacaugaaca accagcacgc ccucgacaag     3300

aacaaggaca agaaggagaa gcagcagagc cggaucgacc ugcgcaccau ccugugcgag     3360

aagaagaaca acaacuggca guucaagaag cccuggucca ccuucacgca ggagaccaag     3420

gaggcgcucg agcacaucgu ggugagcuuc aagcagaacc ugcgggucau caacaaggcc     3480

accaacuacu acgagaagug ggaggagaag aacggcgugc uggugaagaa gaaggucaag     3540

caggagggga ccaacugggc cauccgcaag ccccuccaca aggagacggu guccggcaag     3600

aucgugcugg accgcaagau cggcaaggac aagauccuga ccgccacccg gaaggagguc     3660

gacaucaccu ucacggagaa gaucaucagc uccaucaccg acaccgggau ccagaagauc     3720

cuccugaacu accugaacua caagggcagc cccgaggugg cguucuccgc cgaggggcuc     3780

gaggagcuga acaagaacau cgcccaguac aacgacggca agaagcacca gccgaucuac     3840

aaggugcgca ucuucgagga ggggagcaag uucccccugg gcgagaccgg cgccaaggcc     3900

acgaaguucg ucgaggcggc caaggggacc aaccucuucu ucggcgugua ccaggggaag     3960

gagaagcgga gcuacgccac caucccccug aacgagguga ucgagcgcca gaagcagggc     4020

cugcccuccg ucccggagcg gaacgagaag ggggagcccc uccuguucag ccuguccccc     4080

aacgaccucg uguacgugcc cauggagggc gagaucgccg agaccaucga cuucaacaac     4140

cugagcaagg agcagaagga gcgcaucuac aagacggucu ccuucaccgg caaccagugc     4200

uucuucgugc aggaggccgu ggcgaccccg aucgucaaca agauggagua cagcccccug     4260

aacaagaugg agcgggacau ccucgggacc augaucaagg aggugugcau caagcugaag     4320

guggaccgcc ugggcaacau caccaaggcc uga                                  4353


<210>  2219
<211>  4353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2219
augaagcaca uccugggccu cgaccugggg agcaacucca ucggcugggc cuucgugcag       60

caggacuucg agaacaagca ggggaagauc aucgccgcgg gcagccgcau cauccccaug      120

gaccagaaga ugcuguccaa cuucgaggag gggaccaccg ucagccagac ggccgagcgg      180

accaccuacc gcuccaugcg gcgccuccgg gagcgccacc uccugcggcg cgagcggcuc      240

caccgcgugc ugcacauccu gggcuuccuc ccgccccacu acgacgccca gaucgacuuc      300

accaagcggu acggcaaguu caucgacaac gccgagccca agaucgccua caacaacggg      360

aacuucaucu ucaugaacag cuucaacgag augaucgagg acuucaagga gcaccagccc      420

cagcuguucu acaagaaguc caacggcgag gagagcaaga ucccguacga cuggacgauc      480

uacuaccugc gcaagaaggc gcucucccag aagaucaccc agcaggagcu ggccuggcug      540

auccuccacu ucaaccagaa gcggggguac uaccagcugc gcggcgagga ggagaccgag      600

aaccccaaca aggagguggc cuuccacagc cugaaggucg uggacgugga ggccgaggcg      660

cccaacaaga agggggagau cugguacacc auccggcucg agaacggcug gaucuaccgc      720

cggacgucca agaucccccu ggacgacugg aagggcaaga cccgcgacuu caucgucacc      780

accgaccuga acgacgacgg gacgaucaag cucgacaagg agggcaagga gaagcggagc      840

uuccgcgccc cggacgagaa cgacuggaac cugcugaaga agaagaccga gcacgacauc      900

gacuucuccg ggaagcccgu gggcaccuac aucuacgacc accuccugca gaaccccaac      960

cagaagauca agggcaagcu ggugcggacc aucgagcgca aguacuacaa ggacgagcuc     1020

aaggccaucc uggccaagca gaucgcccug cagcccgagc uguucacgga gacccugcug     1080

gcggacugca uccgcgagcu cuaccggaag aacgagaccc agcagcagaa ccugcugagc     1140

aaggacuucc uccaccuguu cgucgaggac aucaucuucu accagcgccc gcugcggucc     1200

aagaagucca ccaucgccaa cugcacgcuc gagaagcgcu ccuacaucga caaggagacc     1260

aacgagcgga aggacacccc caucaaggug ugcagcaagu ccaaccccua cuaccaggag     1320

uuccgcgugc ugcaguggcu gcagaaccuc cggaucuacg agaucgagac cgaccgcgag     1380

gucacggccg aguucaucga gaccgcccag gacuacgagg cccuguucaa cuuccugaug     1440

agccagaagg agaucgacaa cgagagccuc cugaaguacu uccuguucgc gaagaacccc     1500

cagaucaagg acaagaccgc caagaucgag cucaagaagu ggaucaccac guaccggugg     1560

aacuacgugu acgacguggc cgacaacucc agcaagaagu acccgaugaa cgagaccggg     1620

uacgaccugc agcgcuacau gcagaagguc gagggccugc ccaccaacuu ccugaccuuc     1680

gacacgaccu accggcucug gcaccuccug uacuccguga aggacaaggu ggaguucgag     1740

aaggcccuca agaaguucgc caagaagaag cagcuggaca agaccagcuu cgucgacucc     1800

uucaagaacu ucaagcccua ccccaacgag uacgggagcu ucuccgagaa ggcgaucaag     1860

aagcugcucc cgcugaugcg cuucggcaac cacugggacu ucaaccggau cgacgugagc     1920

acccagaagc gcaucgacga ccugaucaac gggguggcca acgaggacau ccggacgauc     1980

auccgcgaga aggccgagaa guaccagcuc aacuccgaga ccugcuucca ggaccugccc     2040

cuguggcucg cccaguacgu cguguacaac cggcacgccg aggcgaccga caucgagaag     2100

uggaccagcg uguccgaccu ggaggccuac cuggaggacu ucaagcagca cagccuccgc     2160

aaccccaucg ucgagcaggu cgugaccgag acgcugcggg uggugcgcga caucuggcag     2220

caguacggcg gcgggcaggc caacuucuuc gacgagaucc acaucgagcu gggccgcgag     2280

cucaagaaga ccgccgagga gcggaagcag cuguccgaca agaaccagga gaacgagaac     2340

accaaccugc gcaucaagaa gcuccuggcg gagcugaagg aggacgccag cauccagaac     2400

guccggcccu acucccucgu gcagcaggag cugcugaaga ucuacgagga ggggguguuc     2460

agcuccaccg acgagaucga ggaggacauc cagaagaucc gcaagaaggu cgagccgacg     2520

acccccgaga uccagcggua caagcucugg cuggagcaga aguacaagag ccccuacacc     2580

ggcgagguga ucucccugac caagcuguuc acggaggagu acgagaucga gcacaucauc     2640

ccccagagcc gcuacuacga cgacagccug uccaacaagg ugaucugcga gagcgccguc     2700

aacaagcuga agaccaacca gcucgggcug gaguucaucc agaaccacca cggcgagaag     2760

gugcggauca ccgccaacaa ggaggugacc aucuucacgg aggagcagua caaggaguuc     2820

gucaagaagc acuacgccaa caacuccgcg aagcagcaga agcugcuccu gaacgagauc     2880

ccggagaaga ugguggcccg ccagcugaac gacacccggu acaucagcaa guacaucucc     2940

gagauccuca gcaagaucgu gcgcuccgac gagaaggacg agggcgucaa cagcaagaac     3000

aucauccccu gcaccgggaa gaucaccacg gcccugaagc aggacugggg ccugaacgac     3060

guguggaacg agcucauccu gccccgguuc gagcgcauga accagcugac ccagacccag     3120

cuguucaccu ccuacaacga ccggcuccag aagaaccugc cgacggugcc caucgaguac     3180

agccaggggu uccagaagaa gcgcaucgac caccggcacc acgccaugga cgcccucguc     3240

aucgcgugcg ccacccgcga gcacaucaac uacaugaaca accagcacgc ccuggacaag     3300

uccaaggaca agaaggagaa gcagcagagc cggaucgacc ugcgcaccau ccucugcgag     3360

aagaagaaca acaacuggca guucaagaag cccuggcgga ccuucacgca ggaggccaag     3420

gaggcccugg agcacaucgu gguguccuuc aagcagaacc ugcgcgucau caacaaggcg     3480

accaacuacu acgagaagug ggaggagaag aacggcgugc ucgugaagaa gaaggucaag     3540

caggagggga ccaacugggc cauccggaag ccccugcacg aggagaccuu cagcggcaag     3600

aucucccuga accgcgagaa ggugggcaag gggaacaucc ucacggccac ccgcaaggcc     3660

guggacagcu ccuucgacgu caagaagauc agcaagauca ccgacaccgg cauccagaag     3720

auccugcuga acuaccucaa guacaagggg agcccggagg uggcguucuc cgccgagggc     3780

cuggaggagc ugaacaagaa caucgcccag uacaacaacg gcaagaagca ccagcccauc     3840

uacaaggugc ggaucuacga gaaggggagc ggccgcuucg uccucgggga gaagggcacg     3900

aaguccaaga aguacgugca gggggccccc aaccuguacu ucggcgugua ccagggcaag     3960

gagaagcgga ccuacgccac caucccccug aacgagguca ucgagcgcca gaagcagggg     4020

cucccgagcg ugcccgagcg gaacgagaag ggcgagcccc ugcuguucuc ccucagcccc     4080

aacgaccugg uguacguccc gauggagggg gaggcgaccg agacgaucaa cuucaacaac     4140

cugaacaagg agcagaagga gcgcaucuac aagaccgugu ccuucaccgg caaccagugc     4200

uucuucgugc aggaggccgu cgccagcccc aucgugaaca agauggagua cagcccccuc     4260

aacaagaugg agcggagcau cguggggauc augaucaagg aggucugcau caagcugaag     4320

guggaccgcc ugggcaacau caccaaggcc uga                                  4353


<210>  2220
<211>  4362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2220
augaagaccg ugcugggccu cgaccugggg accaacagca ucggcugggc ccugauccag       60

cacgacuucg acuccaagaa gggggagauc cucggcaugg ggagccgcau cauccccaug      120

ucccaggaca uccugggcga guucggcaag gggaacagcg ucucccagac ggccgaccgg      180

accaaguucc gcagcgcgcg gcgccugcgg gagcgccacc uccugcggcg cgagcggcug      240

caccgcgugc ucaacauccu gggcuuccug ccgcggcacu acgccgccga caucgacuuc      300

gagaagcgcc ucgggcaguu cuucgagggc aaggagccca agcuggccua cgacaacaac      360

caguucaucu ucaccaaguc cuucgggaag augcuggccg acuuccggca gcaccagccc      420

gacuuccuca aggacgagaa guccaacgac cugcugaucc ccuacgacug gagcaucuac      480

uaccuccgca aggaggcgcu gaccaagaag aucgagaagg aggagcuggc cuggaucauc      540

cucaacuuca accagaagcg gggcuacuac cagcugcgcg gcgaggagga ggaggagaac      600

cugaacaagc ucguggaguu ccacagccug aagaucaucg acgucguggc cgacgagaag      660

cugaacaaca agggggagac gugguacucc cuccugcugg agaacggcug gaccuaccgg      720

cgcagcucca aggugccccu cuucgacugg aaggagaagg uccgggacuu caucgugacc      780

accgacauca acgacgacgg gagcgugaag acggacaagg agggcaacga gaagcgcucc      840

uuccgggccc ccagcgacga cgacuggacc cugcugaaga agaagaccga gcaggacauc      900

gacaaguccc gcaagcccau cggcgcguac aucuacgacg ccauccucaa gaaccccaag      960

cagaagauca acgggaagcu gguccgcacc guggagcgga aguucuacaa ggaggagcug     1020

aagcucaucc ugcagaagca gaaggaguuc cacccggagc ugcagagcac ggaccucuac     1080

aacgagugca uccgcgagcu guacaagaac aacgacgccc accagcugca gcucaacaag     1140

aaggacuucg ugcaccuguu ccuggaggac aucaucuucu accagcggcc ccuccgcucc     1200

cagaagucca gcgucggcaa cugcccccug gaguuccgga aguucaagga cagcgagggg     1260

aucgagaagg uggaguaccu gaagaccauc cccaagagca acccguacuu ccaggaguuc     1320

cgcaucuggc aguggaugua caaccucucc aucuacaaga aggacgacga cgagaacgug     1380

acccgggacu uccugaagac caucgaggac ugggaggacc uguucgaguu ccugaaccac     1440

cgcaaggaca ucgagcagga gacgcuccug aaguuccugc ucggcaagaa cggggucaag     1500

ggcaaggccc ugaaggugga ggccggcaag uuccggugga acuacgugag cgacaagauc     1560

uaccccugca acgagaccaa ggcgcugauc aucuccaagc ucgagaaggu caagggggug     1620

gaggagaagu uccugaccga ggagaucgag uacaagcugu ggcaccucau cuacagcgug     1680

accgacaagg ucgaguacga gaaggcccug aaggccuucg ccggcaagca gaagcuggac     1740

cucguguccu ucguggacag cuucaagcgc uucccgcccu ucaagaacga guacggggcc     1800

uacuccgaga aggcgaucaa gaagauccug ccgcugcucc ggacgggcaa gagcuggaac     1860

uggauggcca ucgacuccaa gguccgcgac cggaucaaca agaucaucac cggggaguuc     1920

gacgaggaga ucaagaacaa ggugcgcgag aaggccgaga agcacagccu gaagaaggag     1980

aacgacuucc agggccugcc ccucuggcug gcccaguacg ucguguacgg ccggcacucc     2040

gaggccagcu ucuccgggaa guggaacagc guggacgacc ugaagaagua ccucgaggag     2100

uucaagcagc acucccugcg caaccccauc gucgagcagg uccugaccga gacccuccgg     2160

gugguggcgg acaucuggca guucuacggc aagggggaga aggacuucuu cagcgagauc     2220

cacaucgagc ugggccgcga gaugaagaac acggccgagg accggaagga gauguccgcc     2280

aucauccagg ccaacgagac caccaaccug cgcaucaagg cccuccugaa cgagcugcuc     2340

caggacaaga agguggagaa cgugcgcccc uacagcccga gccagcagga gauccugaag     2400

aucuacgagg acgggguccu guccagcgac aucgagaucc ccgacgacau ccagaagauc     2460

uccaaggcgg cccagcccac caagagcgag cuccagcggu acaagcugug gcuggagcag     2520

aaguaccgcu cccccuacac gggcgccaug aucccgcucg gcaagcuguu caccagcgag     2580

uacgagaucg agcacaucau cccccagucc cuguacuucg acgacagcuu cuccaacaag     2640

gugaucugcg agagcgccgu gaacaagcuc aaggacuccc ggcuggggau ggaguucauc     2700

aaggagugcc acggcauggu cguggagacc ggguucggca agagcgugac cgucuucgag     2760

gaggagacgu accgcgacuu cguggugcag aacuacucca agaaccacag caagaagucc     2820

aagcugcucc uggaggagau ccccgagaag augaucgagc ggcagaugaa cgacacccgc     2880

uacaucagca aguucaucuc cagcguccug agcaacaucg ugcgggagga ggugaacgac     2940

gacggcguca acuccaagaa caucgugccc gggaacggca agaucaccac ccagcucaag     3000

caggacuggg ggcugaacga caucuggaac gagcugaucc ucccgcgcuu cgagcggcug     3060

aacgugcuga cggacagcaa gcacuucacc gcgugguccg agaaccacca gcgccuccug     3120

cccaccgucc ccaucggccu gagcaagggg uucuccaaga agcggaucga ccaccgccac     3180

cacgcccucg acgcccuggu gaucgccugc gccagccgga accacaucaa cuuccugaac     3240

aacgcgcacg ccaucgacaa gaagaagaac uccgaggaga agcagaaguu ccgccacgac     3300

cucaaggcca uccugugcga caagaaguac agcgacaagu ccgagaagaa cuaccggugg     3360

aucuucaaga agccguggga caacuucacc aucgacagca agaacgcccu ggacaagauc     3420

aucguguccu ucaagcagaa ccuccgcauc aucaacaagg ccaccaaccg guacgagaag     3480

ugggucgacc gcgacggcau caaggugaag gaguggcaca agcaggaggg caucaacugg     3540

gcgacgcgga agccccugca caaggacacc gugagcggga aggucgaccu gaagcgcgug     3600

accgugcccg agggcaagau ccucaccgcc acgcggaagu cccuggacac cagcuucgac     3660

cugaagguca ucgaguccau caccgacacc gggauccaga agauccucaa gaacuaccug     3720

acgagcaagu ccaacaaccc cgagcuggcc uucagcuccg agggcgugga ggacaugaac     3780

aagaacaucc gcaaguacaa cgacgggaag cuccaccagc cgaucuacaa ggugcgcauc     3840

uucgagcugg gcagcaaguu cccccugggc caguucggga acaagaaguu caaguacguc     3900

gagaccgcca agggcaccaa ccucuucuuc gccguguacg aggacgagaa caagaaccgg     3960

aacuacgaga ccaucccccu gaacgugguc aucgagcgcc agaagcaggg gcugagcucc     4020

gugcccauca agagcgagaa gggccacaag cuccuguucu accugucccc gaacgacauc     4080

guguacgucc ccgggagcaa cgaggaguuc uccaucgacc ggcucuaccg cuucacggac     4140

agcuccgaca agaccgcgaa cuucaucccc cugagcgugu ccagccugau cuucuccagc     4200

aacaagaacg agcagaagaa gaucggcauc uccuacccca uccaggacga guucggccuc     4260

gggagcccgc aguccaagaa ccagaagucc aucgacggca ucaugaucaa ggagaagugc     4320

gugaagguca acaucgaccg gcuggggcgc gugagcuucu ga                        4362


<210>  2221
<211>  4368
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2221
auggccaaga uccugggccu cgaccugggg accaacagca ucggcugggc cguggucgag       60

gaccagaaca acaagaccac gcugaucgac aagggggugc gcaucuucag cgagggcgug      120

aagaucgaga aggggaucga gaaguccaag gcggccgagc ggaccgacuu ccgcagcgcc      180

cggaagauca aguuccgccg gcgccuccgg aagcaggaga cccugaaggu ccugaucgag      240

aacaacaugu gcccccucac caucgaggag uggacgaagu ggaagaagga caagaaggag      300

uacccgaaga accccgacuu caucaacugg cugaagaccg acgagcugaa gaacaucaac      360

cccuacuucu uccgcgacaa ggccagccgg accgagcucc cccugaugca gaccggccgc      420

gcccuguacc accucgcgca gcggcgcggc uuccugucca accggcugga ccaggggagc      480

gacggcuuca cggagcagau ccgccucgac cugaucaaga agaucgagug cgagaccgac      540

aagaccgagc ugcucaccuc cgugcagagc aucaucgaca cguacuccga ggaggaggac      600

aaggacgcca agaagcugcu gaagggggug gagaagcucu gcaagaucaa caacgagcug      660

ccgaucaccg aguucaagga gaagcugauc aacuaccuca acaaggccga caaccugggc      720

gccgucaaga aggggaucgc ggacgugagc aagaagauca ccgagaccgg cugcgagacg      780

cugggccagu acuucuacaa gcucuaccag aaccagucca agauccggaa gguguacacc      840

gcccgcaccg agcacuacua ccacgaguuc cugaagaucu gcgagaccca gaacaucugc      900

gaccagcuga agcacaagcu cgacaaggcc aucuucuacc agcggccccu caagagccag      960

aaggggcuga ucggcaagug cgucuucgag aagaacaaga cgcgcugcgc cgugucccac     1020

cccgccuacg aggaguaccg ggcgcuccag uucaucaaca gcaucaaggu guccgagacc     1080

aaggacggga agaaggacuu ccugagcuuc gacgagcgca agaagaucug gcccaaguuc     1140

auccggaagu ccaagccgaa cuucgacuuc aaggagaucg ccgagacccu ggacaagaag     1200

ggccucagcc gcuacuacaa cuacaccggc cacacguccg ucagcgggug ccccaccauc     1260

gcccagcuga ccaacaucuu cggcgagaac uggcaggaga cccuguucga gaaguacacg     1320

aacaagcaga acaccaaggg gcucaagacc aucgacgagg uggugaccga caucuggcac     1380

guccuguuca cguucgacaa ggacgagaag aucaucgagu ucggcaagca gaagcugggg     1440

cucaacgagg ugcuggccaa gaaguucggc gccaucaacc ugaagaagga cuacgcgagc     1500

cucucccuga aggccaucaa caagauccug cccuaccucc gcgagggccu gaucuacagc     1560

uacgccgugu uccuggccaa caucgagaac gucguggggg aggagaucug gaagaacgac     1620

gagaacaaga accugaucaa gaaggagcuc caccuccuga ucgacaacuu caacgagauc     1680

aagggccugg aggccguggu caacgacuuc aucgcggacu ucaagaagga guacaacaac     1740

gcccacaagg acuacgugcu ggacgagcag gacaagaaga uccugcucga gaagaucgag     1800

uccaacuacg ggaagaacaa guucgagagc cuggagcccg acaagaagac cgagaucauc     1860

aacuccaccg agaagauggu ggccgagcag cugcggaagc agcgcggcga guucaucaag     1920

ccgaagcggc ucgacgagaa gaucagcgag uuccugauca acaacuucaa cgucaccaag     1980

aaguccaucg cccgccugua ccaccccagc gacguggaga ucuucaagac ggccucccgg     2040

aacagcgagg ggcuccugcu gcucggcucc cccaucguca gcuccaucaa gaaccccaug     2100

gcgaugcgcg ccaugcacca gcugcggaag cuggugaaca cccucaucaa ggagaacauc     2160

aucgacgagg agaccaagau caacaucgag cuggcccgcg agcugaacga cgccaacaag     2220

cgggcggcca ucaagaagug gcaggacgag cgccagaagg cccgggagga guacaagaag     2280

uccaucaagg agcuguucaa ggccgagugc ggcaaggaca ucgagccgag cgacagcgag     2340

auccugaagu acgagcugug ggaggagcag cagcacaucu gcaucuacac cgggaagacc     2400

aucaacaucu gcgacuucau cggcgccaac cccaacuucg acaucgagca cacgaucccc     2460

aagucccucu gcuucgacaa cagcaacgag aaccugaccc ugugcgacau ccaguacaac     2520

cgcgacguga agcggaacaa gauccccacc gagcucuccg acuacaacag caucaagccg     2580

cgccuggaca aguggcuggu cuucaucgac gacuacgaga agcucuacaa cacccggaag     2640

aaggcgaagg ggaucgagac gaaggagcag aaggacaagc gcaucaagga gaagcacuac     2700

uacaagcugc accuggacua cuggaagggc aaguacaagc gguucaucau gguggacgug     2760

cccagcgggu ucaagaacuc ccagaucguc gacaccggca ucaucaccaa guacgccauc     2820

agcuacauga aguccaucuu ccccaaggug uacagcguga agggcaacau cgucgccgag     2880

uuccgcaaga ucugggggcu ccagaacgac uacgagaaga aggagcgggu gaaccacgug     2940

caccacugca ucgacgccau caccaucgcc ugcaugucca agcagaagua cgacacgcug     3000

gcgaagaucu accacgagga cgaggccggc uuccacaaga ucaagaagga gacccagcug     3060

gucagcaagc ccuggaccuc cuucaccaug gacgugcuca acaucgagaa ggagaucuuc     3120

gugagccacc acacgccgga caacaugaag aagcagucca agaaggucgu gcgcgagcgc     3180

gggaucaucg cccccaaggu gaucuacgag accgacgcca acaacaacac ccugcgggac     3240

aaguacggca agaagaucgu caaggaguac auccugcuca aggacgugaa cggcaaccug     3300

auccccauca aggggaagcg ccugaccccc agcgacauca ucaacaaggu ggacggcauc     3360

gacuacuucu cccucaucau caacgacaag auccaguacu acgaguucgc caagagcaag     3420

aagacgggga agaucaucua caagaaggag ccgaucuacc ugcagggcga caccguccgg     3480

gggucccugc accucgacac cuucuacggc gcgaucgcca aggacaucaa cggcaacauc     3540

aucaccaaca cgaacgggaa gaucgccccc aacuacguga uccgcaagga gcugagcaag     3600

cugaaggaga ccgacaucga gaacaucgug gaccccgucg ugcggggcaa gaucaucgag     3660

gccaagaaga acaagcucau caccuucaac aaguccgggg cggugaucaa gaccacgauc     3720

uggaugaagg agaaccagau cccgaucaac aagguccgcg uguacagcuc ccugaagaac     3780

ccccugcccg acuucaagaa gcaccgggac cucagccacc ugaccuacaa gcagcaguac     3840

aacgugagca acgaggagaa cuacugccug gccaucuacg agggcaagaa caagaagggg     3900

aacgucugcc ggaccugcga gcucgugaac augaucgacg ccggcaacua cuaccgccug     3960

uccaacaaga acuggaagca gcaguacccc aucgugccgg agaaggacag cgucaccaac     4020

uacucccuga agacgaucau caccaagggc cucauggugc ugcuguacga gaaguccccc     4080

gaggagaucu gggagcucac cuacaaccag cagauggagc gccuguacga gaucacccag     4140

cuggacacgg agaaguccgc cgagaucaag cuccuguucc accaggaggc ccgggagaag     4200

aaggugauca ccgaguacau ggggcugaag accggcauga agggcgggaa gaacaucggg     4260

aagcacaagg aguuccccug gaucaagguc ggccccaaca gcuucgacgc gcucaucgag     4320

ggcaucgacu ucaagcugac cccgacgggg aagaucauca agcuguga                  4368


<210>  2222
<211>  4374
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2222
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc caaggugucc       60

gucgacgacu acgggaacua ccugcacgac aucaagcucg gcagccgcau cauccccaug      120

ucccaggacg ugcuggggaa cuucgacagc ggcgugacgg ucucccagac cgcccagcgg      180

accgaguucc gcagcaagcg gcgccuggug cagcggaacc uccagcgccg ggagcgccug      240

caccgggugc ugcacguccu cgacuuccug ccggcgcacu acgacgccgc caucggcugg      300

gaccgcaccg aggccgugac guacgggaag uuccugaacc acgccgagcc caagcucgcg      360

ugggaggagc ugggcaacgg gaagcggcgc uuccuguuca uggacuccuu ccacgagaug      420

guggccgacu ucgcccggug ccagcccgcc cucgucgcgg acggcaagaa gaucccccug      480

gacuggacca ucuacuaccu gcgcaagaag gcccucaccc aggccaucac caaggaggag      540

cuggccugga uccugcucaa cuucaaccag aagcgggggu acuaccagcu gcgcggcgag      600

gaggaggagg agaacccgac gaagcaggag gaguacuacg agcugaaggu ggugagcguc      660

gaggccaccg acucccggaa gggcaacgac accugguaca acgugcaccu cgagaacggg      720

uggaucuacc gccggagcuc caagaucagc cuggacgacu gggugggcaa ggucaagcag      780

uucaucguga ccacggagua cgagaaggac ggggcgaccc ugaagaccga caaggagggc      840

uacgugaagc gcuccuuccg ggcccccaac gagaacgacu ggggccuccg caagaagcgc      900

accgagacgc ugcuggagau gagcgggaag accgucggcg ccuucaucua cgaccaccuc      960

cugcaggagc ccgccgacaa gauccggggg aacuucaucc gcaccaucga gcggcacuac     1020

uacaaggccg agcuggaggc gauccucgcc gagcagugca aguaccacau cgagcugcgc     1080

gacaaggaga ugcuggaggc cugcgcccgg gagcucuacc ugaacaaccu gccccaccag     1140

cgcucccuca ugaagaagga caugaccuac cugcugcucu acgaccugau cuucuaccag     1200

cggccgcuca agagcaagaa ggcccugauc gcgaacugcc ccuacgagua cuacgaguac     1260

guggacaagg agacgggcga gaucaagcgc cagaacauca agugcaucgc caaguccaac     1320

cccuacuucc aggaguuccg gcuguggcag uucgugcuga accuccgccu guucgaccgg     1380

gucgaggaca aggaggugac cgccaacuac cugcccaccg ccgacgacca cgcccgccug     1440

uucaccuacc ucaacgaccg gaaggagauc aaccaggaga cgcugcugaa ggacuacuuc     1500

aagcucaaga aggugaagau cgggaacgag aaggaguacc cgauccgcug gaacuacauc     1560

gaggacaagg agaagaagua ccccugcaac gagacccggu acgagcugcu gauggcgcuc     1620

gaccgcgccg gcaucgagca caaguggcug gacgacaaca accuggagua ccggcucugg     1680

caccuccugu acagcgucga ggugaagucc gagagcgaga ccgcccugcg caagcugaag     1740

aacgacgacg ccuucgucaa cagcuuccug aaggugaagc ccuucaagaa ggaguacggc     1800

gcguacuccg agaaggccau caagcggcuc cuggccguga ugcgcaaggg guuccugugg     1860

aacgagcagg acaucugcga cgagacccgc aagcggaucg agaucaucau caagggcgac     1920

gucgacgaga aggugaagga gcgcaugggg cacgccgccu acagcuucga ccgggugucc     1980

gacuuccagg gccuccccga guggcuggcg ugcuacgucg uguacgggcg ccacagcgag     2040

gccauggaca uccagcacug ggacuccccg gagcggcuga uggccuacau caacaacuuc     2100

aagcaguaca gccuccgcaa ccccaucgug gagcagugca uccuggagac gcugcggacc     2160

guccacgaca ucuggaagga gaccggccag aucgacgaga uccacgugga gcucggccgc     2220

gagaugaagu ccaccagcga ggagcgggcc cgccggacgc agaacgugcu gcgcaacgag     2280

aacaccaacc ugcggaucaa gucccuccug aucgagcuga agaacgacac cgacaucugc     2340

aacguccacc ccuacagccc caugcagcag gagauccucc gcaucuacga ggagggggcc     2400

cugcaggagc ugaaccggga ggacgaggac uucgaggaga ucuccaagau cagccagaug     2460

ucccagccga gcggcaagga gcucaaccgc uacaagcugu ggcuggagca gaaguaccgg     2520

ucccccuaca ccgggaaggc gaucagccuc gccaagcugu ucacgcccgc cuaccagauc     2580

gagcacguga ucccccaguc ccgcuacuuc gacgacagcu ucaccaacaa ggugaucugc     2640

gaggccgagg ucaacacccu gaaguccaac cuccugggcu acgaguucau caagaagcac     2700

gggggcgaga ucauccacug caccacccug ggcgacgugc ggaucuucag cgagagcgag     2760

uacaaggccu ucgugaugga ccacuacgcg aacaacaagu ccaagaagga caagcuccug     2820

auggaggaca ucccgcagga guuccugagc cgccagauga acgacucccg guacaucagc     2880

aaggucguga agacgcuccu guccaacauc gugcgcaugg ccgacgagag cgaggccacc     2940

uccaagagcg ucaucgugug caccgggggc gugaccgacc gccugaagaa ggacuggggg     3000

cucaacgacg ucuggaacuc caucguguac agccgguucg agcgccugaa ccggcugacg     3060

ggcaccgagg ccuucggcca cugggagaac aaggacggga agcgcguguu ccagaccucc     3120

augccccucg agcugcagcg gggcuuccgc aagaagcgga ucgaccaccg ccaccacgcg     3180

auggacgccc uggucaucgc cugcgccagc cggaacaucg ugaacuaccu caacaacgag     3240

uccgccaaca accccaagaa gcgcgaggac cugcggcgcc agcugugcga caagaaccgg     3300

gugauccgca agcccuggga gaccuucacg caggacgcgu acaacgcccu cgagaacguc     3360

guggugagcu ucaagaacua cguccgggug aucaacaagg ccaccaaccg cuacgagcac     3420

uacgacgaga acgggaagaa gcgguucgug cagcagaagg gcuccgagcu gugggccauc     3480

cgcaagccga ugcacaagga gaccuacuac gggcacguca accugcggcg caaggagcuc     3540

gugacccugc agaaggcccu ggacgacauc cccagcaucu gcgacaagaa gcuccgcgac     3600

uacaucuccg cgcuggugga gaagcacuuc aacaagcggc agcugcucgc ccacuucaag     3660

agcacggacu acuacuggca gaagcaguac gucaacaagg uguacgugug gaccuucagc     3720

gacgacauca agccccuggu cgccacccgc aagcugcucg acaccuccuu cgacgccaag     3780

cggaucggca cgaucaccga caccggcauc cagaagaucc ugcgcaacua ccuggaggcc     3840

aagggcggga acgcgacccu cgccuucacg ccggagggga ucgccgagau gaaccagaac     3900

aucagcaagu acaacgacgg caagccccac cagcccaucc ugaaggugcg gaucaccgag     3960

cccauggggg ccaaguaccg cgugggccag accggcaaca agauggcgaa guacgucgag     4020

gcccaguccg ggaccaaccu guacuucgcc aucuacgagg acagcgaggg caagcggucc     4080

uacacgaccg ugccgcucga cgagguggcc gagcgccuga agcagcggcu gagccccguc     4140

cccgagaagg acgagaacga cgugccccuc aaguucuacc uguccccgaa cgaccuggug     4200

uacgucccca ccgaggagga gcuccagcug cgcgagugca agcugaacaa ggaccggauc     4260

uacaagaugg ugagcuccac cgggaacgag ugccacuuca uccccuacua cgucgccaac     4320

gugaucuaca acaagggcgg guucaacaug cagaugcacc gcccccagag cuga           4374


<210>  2223
<211>  4374
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2223
auggccaaga uccugggccu cgaccugggg accaacagca ucggcugggc caucguggag       60

aagaacaacu ccgacuucac ccugaucgac aagggggucc gcaucuucag cgagggcgug      120

aagacggaga aggggaucga gcggucccgc gcggccgagc ggaccggcua ccgcagcgcc      180

cggaagauca aguaccgccg gaagcuccgc aaguacgaga cccugaaggu gcuguccaag      240

aaccggaugu gcccccucca gaucgaggac gucgaggccu ggaagaaguc cggcuucaag      300

gacuacccgc ugauccccga guuccugcag uggcuccgca ccaacgacca cgagaacgug      360

aaccccuacu ucuuccggga caaggccucc cgcgcgaagg ugagccugug ggagcugggg      420

cgggccuucu accacaucgc ccagcgccgg ggcuuccucu ccaaccgccu ggaccagagc      480

uccgaggggg uccuggagga gcacaacccc cagauccaga gccucaucga ggaccuggag      540

gagacgucca ccauccugaa cgaggugcgg gacuacuaca ucaaccucgg ccugcuggac      600

ggggugaagg gcgacuucaa gaaggagcuc gacgagggcg agaagaagcu caagagccug      660

uacaacaccc ugaucgccau caucaagaag aacgagaacg acguccagaa gugcaaggac      720

gagaucauca agcgccugaa caagaaggag gaccugggga aggugaaggg caagaucaag      780

gacaucuccc aggcgaugcu cgacgggaac uucaagaccc ugggccagua cuucuacagc      840

cuguacaaca agggcaagau ccggaaccag uacacguccc gcgaggagca cuaccuccag      900

gaguucauca ccaucugcaa ggugcagggg aucaacggca ucaacgacga ggagauccug      960

ccggagaaga aguucaccgg gcuggccaag gaccucuaca aggccaucuu cuuccagcgg     1020

ccccucaaga gccagaaggg ccugaucggg aagugcuccu ucgagaagaa caagagccgc     1080

ugcgccauca gccaccccga cuucgaggag uaccgcaugu ggaacuaccu gaacaccauc     1140

aaguucggca cgcaguccga caagaagcug cgguuccuga cccaggagga gaagcucaag     1200

cugaucccca aguucuaccg caagaacgac uucaacuucg agguccuggc caaggagcuc     1260

aucgacaagg gcgcgaccuu cggguucuac aagagcuccc ggaagaacga guuccuguac     1320

ugguucaacu acaagccgac cgacacggug agcgccugcc aguucuccgc cagccugaag     1380

aacgccaucg gcgaggacug gcagaccaag guguucaccu acaagacccu caacuccgag     1440

aagaaggagg ucauccgcac gguggacuac cgggaccugu ggcaccuccu gagcgccucc     1500

accagcgaca ucuaccugua cgaguacgcg aaggagaagc uggggcugga ggacaagucc     1560

gccaagaccu ucagcaagau caagcucaag aaggacuucg ccucccugag ccuguccgcc     1620

aucaacaaga uccuccccua caucaaggag ggccugcugu acagccacgc cguguucaug     1680

gcgaacaucg agaaggucgu ggacgagcac aucuggaagg acgugaagga gcgcaccauc     1740

auccagaacg agaucuccaa gaucaucaag agcgacgucu ucgagcggga ggugaucaac     1800

aucaucaacg ggcucaucaa gaacuccaag acggagggcg agaacuacgc cgaggagagc     1860

aagaacaucu ucaagaccga guucgacaac gccaucgcca agaucuucga gaagaacaac     1920

gacaucgagu ucaaccagcc cuuccuggac gacaccuacc ugaaguacgu ggaggagcuc     1980

aagaaguucg acaaggcccg cagcuuccug cccgucccgc ggcuggacga gaaggugauc     2040

caguuccucc ugggcaacaa caaggacggg auggucuacu gcuccgacaa gaacaagacc     2100

aagaagcugu accaccccag cgacaucgag aucuucaaga agaagaucau caaggacgag     2160

uucggcaacg agaagauggu gcucgggagc ccccugacga ccagcaucaa gaaccccaug     2220

gcgaugcgcg cccugcacca gcuccggaag gugcugaaca cccugauccu cgaggagcag     2280

aucgacgaga acacccgcau ccacaucgag auggcccggg agcugaacga cgccaacaag     2340

cgcaagggca uccaggacua ccagaacgag aacaagaagu uccgggagga cgcgaucaag     2400

gagaucaaga agcuguacuu cgaggagugc aagaaggagg ucgagccgac ggaggaggac     2460

gugcuccgcu accagcugug gaucgagcag gacaagaagg agaucuacga gcaggggaag     2520

uccauccgga ucagcgacau caucggcucc aaccccgccu acgacaucga gcacaccgug     2580

ccccgcagca ucucccagaa caacagccug augaacaaga cccucugcac gccccgguuc     2640

aaccgcgaga ucaagaagca gaagaugccg aucgagcugu ccaacuacga ggagauccug     2700

ccccgcaucg agcacuggaa gaaggaggcc gaggaccuca cccggcagau cgagagcauc     2760

uccaagagca ucaaguccgc cagcaccaag gaggccaagg acaagaacau ccgccggcgc     2820

cacuaccuga cccugaagcg ggacuaccuc aagggcaagu acgaccgcuu caucugggag     2880

gagcccaagg ucggguucaa gaacucccag auccccgaca ccggcaucau caccaaguac     2940

gcgcaggccu accucaagag cuacuucaag cggguggaga gcgugaaggg cgggaugguc     3000

gccgaguucc gcaagcagug gggcauccag gaguccuaca ucgacgagaa cggguucaag     3060

cacuacaagg agaaggaccg gagcaagcac acgcaccaca ccaucgacgc caucaccauc     3120

gccugcauga ccaaggacaa guacgacgug cuggcgcagg ccuggacguc cgaggacgag     3180

caggacaagc agaaggccaa gacccugaug agcgaggcca agccguggaa gaccuucaag     3240

gaggacaucg agaagaucga ggaggagauc cugguguccc acuucacccu ggacaacguc     3300

aagaagcaga gcaagaagau cgugcgcgug cggggcaaga agcaguacgu cgccgagauc     3360

gagcgcgacg ugaacgggaa ggcgauggcc aagaaggacg ccaacggcaa gaucaucuac     3420

aagcucaacg agaaggggga gaagaucccc cggcugcagc agggcgacac gauccgcggc     3480

ucccugcacc aggacagcau cuacggggcc aucaagaacc cccucaacca ggaggagauc     3540

aaguacguga uccggaagga ccuggagucc cugaaggcga acgacgucga caacaucgug     3600

gacgagaccg ugaagcagaa ggucaaggag gccaucgaga acaagauccu ccugcugagc     3660

uccaacgccc agcagaagaa caagcucgug gacgacgugu ggcugaacaa ggagaagcag     3720

guccccauca agaaggugcg caucuacgcc aacagcguga agaacccgcu ggagaucaag     3780

gagcaguccc uccugagcaa gucccggaag gagcacaagc agaaggucua cggccagaac     3840

gacgagaacu acgccauggc gaucuacgag cuggacggga agcgcgaguu cgagcucauc     3900

aacaacuuca accuggccaa gcugaucaag cagaaggagg gcuucuaccc ccucaacaag     3960

gagaaggagg ugaaggggaa gaaggugcug uaccccaucg ccaagagcaa cuaccgggac     4020

gucgugcuga agcgcggcca gcaggucgug uucuacgaca aggaggugga gaacccgaag     4080

gacaucuccg aggugaucga cuucaagggc cggaucuaca ucaucgaggg gcucagcauc     4140

cagcgcaucg uccgccccuc cggcaagauc gacgaguacg gggugaucau gcugaaguac     4200

uucaaggagg cccggaaggc cgacgagauc aagaaggaca acuacaagcc cgacggcgug     4260

uucaagcugg gggagaaccg ccccacccgg aagaugaacc acagccaguu caccgcguuc     4320

gucgagggca ucgacuucaa ggugcucccg acgggcaagu ucgacaagau cuga           4374


<210>  2224
<211>  4377
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2224
augaccaaga ccauccuggg ccucgaccug gggacgaaca gcaucggcug ggcccugauc       60

aaccaggacu ucgacaacaa gaagggggag auccucggca uggggucccg caucaucccc      120

augacccagg acaucaagga cgaguucggc aagggcaaca gcaucuccca gaccgccgag      180

cggacccgcc ugcgcggggu gcggcggcug auccaccgca cgcuccugcg gcgcgagcgg      240

cugcaccgcg uccucaacau caucggcuuc cugccggagc acuacgcgaa ccagaucgac      300

uucgagaagc gguucgggaa guucaagccc gagagcgagc ccaagaucuc cuucgacggc      360

aacgacgugu uccuguucga gaaguccuac caggagaugc ucguggacuu caagauccac      420

cagccccagc uggucagcaa cgggaagaag aucccgcacg acuggaccau cuacuaccug      480

cgcaagaagg cccucagcaa gaagaucgag aaggaggagc uggccuggau ccugcucaac      540

uucaaccaga agcggggcua cuaccagcug cgcggcgagg aggaggagga gacccccaac      600

aagcuggugg aguuccacuc ccucaagauc guggagguca acgccgacga gccccagaag      660

gggaagcccg agaucuggua cagccuggug cuggagaacg gcugggugua ccggcgcgcc      720

uccaagaccc cgcuguucga cuggaaggac aagauccggg aguucaucgu cacgaccgag      780

aucaacaacg acgggaccgu gaagaccgac aaggagggca cggagaagcg cagcuuccgg      840

gcgcccaagc ccgaggacug gacccugcag aagaagaaga ccgaguucga gcuguccaag      900

agcggcaacg aggugggggc cuucaucuac gaguccaucc uccagaagcc caaccagaag      960

auccgcggca agcugaucag caccaucgag cggaaguucu acaaggagga gcugaagacg     1020

auccucaaga cccagcuguu cuuccacaag gagcugaagg acgagaagcu cuacaacgcc     1080

ugcaucgagg agcuguacaa gaacaacgag gcccaccgcu cccugcucag caacaagggg     1140

uucgagcacc uguucaucaa cgacauccug uucuaccagc gcccgcuccg guccaagaag     1200

ucccagauca gcaacugccc ccuggagaag cgcaccuaca agaaggaggg caucgagauc     1260

accgagggga ucaaggucau cagcaagagc aaccccaucu accaggaguu ccggcugugg     1320

caguggaucu ccaaccucag ccuguacugc aucgagccca cggagaccaa cgugaccucc     1380

accuuccuga acagcaucga ggacuacgag aaccucuucg aguuccugaa caaccgcaag     1440

gagaucgagc agaagcaccu ccucaaguac cugcuggaga accagggcuu caagggcaag     1500

cugcucacga acgagcugga gaaguuccgg uggaacuucg uggcggacaa gaaguacccg     1560

ugcaacgaga ccgggucccu gcuccacacc cgccugagca aggucaagaa caucuccccc     1620

gacuuccuga ccaaggagau cgagcaccag cucuggcaca ucaucuacag cgugacggac     1680

aagaucgagu acgagcaggc ccugaagacc uucgcccgga agaacaaccu ggacguggac     1740

uccuucuucg agcacuucaa gaagaucccg cccuucgaga gcaccuacgg cgccuacucc     1800

gagaaggcca ucaagaagcu ccugccccug auccgccucg ggaaguacug gaacugggag     1860

gcgaucgaca gcaucuccaa ggaccggauc agcaagaucc uguccggcga guacgacgag     1920

aacaucaaga accgcguccg ggagaaggcc gugcaccuga ccagcgagaa caacuuccag     1980

gggcuccagg aguggcuggc caaguacauc guguacgacc gccacuccga gggcaacgac     2040

cugggcaagu ggacgagcgu cagcgaccuc gagaccuacc ugaaggaguu caagcagcac     2100

ucccugcgga accccaucgu ggagcagguc aucaccgaga cccuccgcgu ggugaaggac     2160

aucuggauca agcacgggaa gggcacggag aacuucuucg acgagaucca cgucgagcug     2220

gggcgggaga ugaagaacaa cagcgaggac cgcaagcggc ugaccaacac caucaccgag     2280

aacgagaaca ccaaccuccg caucaaggcc cugcugaugg agaugaugaa cgacaacgac     2340

guggagaacg ugcggcccua cucccccagc cagcaggaga uccucaagau cuacgaggac     2400

ggcgcccuga acuccaacau cgagcuggac gacgagaucg ucaagaucag caagaaggcg     2460

gagccgacga aguccgagcu ccagcgcuac aagcuguggc uggagcagaa guaccgcagc     2520

cccuacaccg gccaggugau cccccucaac aagcuguuca ccuccgagua cgagaucgag     2580

cacguggucc cccagagccg guucuucgac gacuccuuca gcaacaaggu gaucugcgag     2640

uccgccguga acaagcgcaa ggacaaccag cuggggcucc aguucaucaa gaaccacagc     2700

ggcgagaagg ucgagcuggg guucggcaag guggugcagg ucuucaccga ggagcaguac     2760

cuggacuucg ugaaggagca cuacuccaag aaccggagca agcacaacaa gcuccugcug     2820

gaggagaucc cggagaagau gaucgagcgc cagcucaacg acacgcggua caucuccaag     2880

uucgugagca gcauccuguc caacaucguc cgcagcgaga aggacgacga cgggcugaac     2940

uccaagaaca uccuccccgg caacggcaag aucaccaccg agcugaagcg ggacuggggg     3000

cugaacgacg uguggaacga ccucauccug ccccgcuucg agcggaugaa caccaucacg     3060

aacagcgacc uguucaccac cuacaacgac aaguaccaga agcaccuccc gaccgugccc     3120

uucgaguacu ccaagggcuu ccagaagaag cgcaucgacc accggcacca cgccauggac     3180

gcccugguca ucgccugcgc gacgcgcgac caccugaacc ucaugaacaa ccagagcgcc     3240

aaguccgagc ugaagcggua cgaccugcag aacaagcucc gcaagaagga gcccuacuuc     3300

aaccagaagg agaacaagca gaaggaggcc uucaaggacu ucaucaagcc cugggggacc     3360

uucaccgagg acagcaagaa cgcccuggag aagaucauca ucuccuucaa gcagaaccug     3420

cgggugauca acaaggccac caacagcuac gaguccuaca aggacgagga cggcaaccuc     3480

cgcaucggga aggacggcaa gccggagaag ggccugauca agcagaaggg gcugaacugg     3540

gcgauccgga agccccucca caaggacacg gugagcggcc agaucaaccu gucccgcauc     3600

aagcugccca acgggaagau ccucaccgcc acccggaaga accuggacac cagcuucgac     3660

cugaagacga ucgagaacuc caucaccgac accggcaucc agaagauccu caagaacuac     3720

cugcugagca aggagucccc cgagcucgcc uucagcccgg aggggcugga ggagaugaac     3780

aacgagaucg agaaguacaa caacggcaag uuccaccacc ccaucaacaa ggcccgcauc     3840

uacgagcugg gcuccaaguu caacgucggg cagaccggca accggcgcga caaguucgug     3900

gaggcggcca aggggacgaa ccucuucuuc gccaucuacc aggacgagaa caagaaccgc     3960

agcuacgaga ccaucccccu gaacgaggug aucgagcacc agaaguggcg ggccagccug     4020

cccaaggagg agcaggagaa gaucccgcuc guccccguga acaagcugaa gggcaccuuc     4080

aucuucuccc ugagccccaa cgaccucgug uacguccccu ccaacgacga gcuggagcgc     4140

agcgccucca ucgacuucag caagcugaag aaggagcaga ucaaccggcu cuacaagaug     4200

guguccagcu ccgggagcca gugcuucuuc gugaaguccg aggucgcgac cagcguggug     4260

aacaagaugg aguacuccag ccugaacaag auggagaagu ccaucgacaa ccugaugguc     4320

aaggagaucu gcaucaagcu caagaucgac cgccugggca acaucagcaa ggccuga        4377


<210>  2225
<211>  4377
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2225
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucgag       60

caggacuucg aguccaaggc cgggaagauc agcggcgcgg ggucccgcau cauccccaug      120

agccaggaca uccucggcac cuucgagacg ggccaguccu acagccagac cgccgaccgg      180

acccacuacc gcuccauccg gcgccuguac cagcggacca agcugcgccg ggagcgccuc      240

caccggagcc ugaacgugcu gggguuccuc ccggagcacu acaagaacga gaucgacuuc      300

gagcagcgca agggccaguu cuaccccaac aaggagccca agaucgccua cuaccccgag      360

caggucgccu ccaaggugaa guaccaguuc cuguucaaga gcuccuuccu ggagauggug      420

gaggacuuca agcggcgcgg guacaacggc gccaucccgu acgacuggac gaucuacuac      480

cuccggaaga aggcgcugga gaagaagauc gagaaggagg agcuggccug gcuccugcug      540

aacuucaacc agaagcgcgg guacuaccag cuccggggcg aggaggagga caccaacagc      600

aacaagaagg aggaguucua cgagcugaag gucuccagcg uggagcagac ccacgacacc      660

ucccccagcg gcacguggua caacgugcac cuccagaacg ggugggucua ccgccggaag      720

uccaaggaca gccugaagga cugggagggc cugaacaaga aguucaucgu gaccacccac      780

cugaacgccg acggguccga gaagcucgac aaggacggca acgugagccg cagcuuccgg      840

gcccccaagg aggacgacug gggccugguc aagaagcgca ccgaguccgu gcuggagaag      900

uccgggcucc acgugggcga guucaucuac gagagccugc uggagaaccc cgagcagaag      960

auccggggga gccucaucaa gacgaucgag cgcaaguacu acaagaagga gcuggaggcg     1020

auccuggaca agcagaccuc cuuccaccgc gagcuccgga acgacgcccu guacaaggag     1080

gccacccugg agcucuaccc gaagaacagc gcccaccgcu ccaacaucaa gcagcggggc     1140

uucaaguacc uguucaucga ggacaucauc uucuaccagc gcccccucaa gagccagaag     1200

caccugaucg cccacugcca guacgagaag cggaccuaca agaacaacgg gggcgagacg     1260

aucgaggucu cccugcaggg cgcgcccaag agccaccccu ccuuccagga guuccgcauc     1320

uggcaguuca ucagcaaccu gcgguucuac gacaaggagg agaucgugga ggggcaggag     1380

guguccauca ccgagaagcu ccucaagagc gacuccgacu ucgccgagcu guaccacugg     1440

cugagccacc agcgcgaggu cgaccagaac aagacccuga aguacuucac ccagaagaag     1500

cugaucccga agcagaagaa ggacaacccc acgauccggu ggaacuuccc cgaggacaag     1560

aaguacgaga ugggcgaguu caaggcccag cuccuguccc gccuggagaa ggugcggaac     1620

ugccagcccg acaaguuccu gacccagaag aacgagcucg accuguggca caucaucuac     1680

agcgugaccg acaagaccga guucgagaag gcccugguca cguucgccga gaagcgcgag     1740

cucgacuccg agagcuucgu ggagaacuuc aagaaguucc cgcccuucaa gaacgacuac     1800

gcgagcuacu cccugaaggc caucaagaag auccugcccc ucaugcggcg cgggaaguac     1860

uggaaggccg agaaccuggg cccccagacc cugcagcgga ucgagaagau cgugaacggg     1920

gaggccgacg acaagaucag cauccgcgcc cggaagcucg cgcugcaccu gaacgacacc     1980

aaguccuucc agggccuccc gaccagccug gccugcuacg ucgccuacaa ccgccacucc     2040

gagaacgagg agaaccagaa guggcagacg cccagcgaca ucgacaagua ccugaaggac     2100

uucaagcagc acucccuccg gaaccccauc guggagcagc ugaucaccga ggcccugcgc     2160

gugguccgcg acaucuggaa gcacuacggc caggggcaga aggacuucuu cgacgagauc     2220

cacguggagc ucggccggga gaugaagaac cccgccauca agcgcaagaa gcugagccag     2280

aagauccagg agaacgagaa caccaacuac cggaucaagg agguccugcg caaccucaag     2340

gagaacaaca ucgacgcgaa gccguacucc cccagccacc aggagauccu gaagaucuac     2400

gaggaggggg uguaccaguc caucgagaac guggaggacg agaucgagaa gauccggaag     2460

aacaacaccc ccagcuccaa ggacaucaac cgcuaccggc uguggcucga gcagggcuac     2520

aucagccccu acacggggga ggucaucccg cugucccagc uguucagcaa ccaguacgag     2580

aucgagcaca ucaucccccg cucccgguuc uucgacgaca gccucgccaa caaggugauc     2640

ugcgagagcu ccgugaacgc ccugaaggac aacaagaccg cccuggaguu caucaccaac     2700

cacggcaacg agaaggucga guucgcgggc aagaccgugg agauccucag cuccgacaag     2760

uacacggagc acugcaagcg cuacuucaag cggaaccgca ccaagcugaa gaaccugcuc     2820

agcuacgacg ugcccgaggg guucaucaac cggcagcuga acgacucccg cuucaucagc     2880

aaguacgucc ggaaccugcu cuccaacauc gugcgcgagg aggaggagcg ggaggccauc     2940

agcaagcaca uccugaccau cccgggcucc aucaccagcc agcugaagca ggacuggggg     3000

cucaacgaga aguggaacga gguggucacc caccgcuucg agcggcugaa caaccugacg     3060

ggcaccaccg acuucggcuu cugggacaac aagaucaacg ccuuccgcau caccgugccc     3120

aaggagcucu ccaagggguu caacaagaag cggaucgacc accgccacca cacgcuggac     3180

gcccugguga ucgccgcgug caccaaggac cacgucaacu acaucaccag ccucaacacc     3240

gagcgcaaca accacucccu ggugagcaag cugcggaaca cgaagaaggu gcucaucaac     3300

ggcaaggagc gccggaucgc caaggccuac aagcugcccu gguccagcuu cccccuggac     3360

gccaagcacc agcucgagac caccgucauc uccuucaagc agaaccugcg cgugaucaac     3420

aagagcucca acaagaccug gcaguggcgg aacgugaacg gggagcagaa gaagaagcug     3480

aucccgcaga cgaagggcga cagcugggcc auccgcaaga gcaugcacaa ggcgaccguc     3540

uccgccaagg uggagagcgu ggggaagggc aagaucaaga ccaccaaccg gacgccccuc     3600

ggcgagauca agggggagaa gcacgucgag aagaucuacg acgcccgcau ccgggaggug     3660

aucaucccca accaccugaa caaguaccug gacgagaagg gcaagcccaa cuacgaggcc     3720

gccuucuccc agagcgggau caagcagcuc aacgagaaca ucgaggugcu gaacgagggc     3780

aagccgcacg cgcccaucua caaggucccc cuguucgagg ugggguccaa guuccccgug     3840

agcgaggacg ccaagucccc gaagaaccag aaguacgucg aggccgacaa gggcaccaac     3900

cucuucuucg ccguguacug gaacgaggag aagcagaagc gcaacuucga gaccgugccc     3960

cugcaccagg ucaucgugca ccagaagcag gucgcgagcc ugcccaccga gcagcggacg     4020

uccgugccca ucaagccgga gcucggccug uuccuguuca gccucucccc caacgaccug     4080

guguacgugc ccaccgagga ggagaucgag aacccccacc uggucgaccu caagaaccug     4140

agcaaggacc agaccaaccg caucuacaag auggugucca gcaccgggaa cgagugccac     4200

uucaucccgc accacaucuc ccggcugauc augaaguaca acgccaagca gaaggugggc     4260

gaguucggga gccucaacaa ggccgagaag gccaucacgg gcgagaccau caaggagaag     4320

ugccugaaga uccagaucaa cucccugggg aagcucaaga ccgagcgcau cgccuga        4377


<210>  2226
<211>  4380
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2226
augaaguuca accugggccu cgacaucggg accaacagca ucggcugggc ccuggugaac       60

gaggacuacg agaacaagaa ggggaagauc aucgccgcgg gcucccgcau cauccccaug      120

acccaggaca uccuggacaa guucgggagc ggcgccauca cggagaccca gaccgcccuc      180

cggaccgacu accgcuccaa gcggaagcug auccagcgcu uccugcuccg gcgcgagcgg      240

cugcaccgcg uccugaacgu gcucgacuuc cugccgaagc acuacgccga gagcaucgac      300

uucgagaagu acuucggcaa guuccugccc aacacggagc ccaagcucgc cuacaaccag      360

aaccaguuca ucuuccagaa cuccuuccug gagaugcuga aggaguucca ggagaagcac      420

cccgagcucc ugaccaacaa caagaagauc ccguacgacu ggaccaucua cuaccugcgg      480

aagaaggcgc ucaaccagcg caucgagaag gaggagcugg ccuggcugcu ccugcaguuc      540

aaccagaagc ggggguacaa ccagcugcgc gacgaggagg aggagagcgu gcccaacaag      600

caggucgagu ucuacucccu caagaucauc gacguggugg ccgaccagcc caaggagggc      660

cgggacaagc gcugguacag caucaaccug gagaacgggu ggaccuaccg gcgcgagucc      720

aagacgcccc uguucaacug ggagggcaag accaaggacu ucaucgucac caccgaccuc      780

aaccaggacg ggagcaucaa gaaggacaag gagggcaagg agaagcgguc cuuccgcgcc      840

ccgagcgagg gcgacuggac gcugcugaag aagaagaccg agaaggagau cuacgagucc      900

gggaagaccc ucggcaccua caucuacgac gcgcugcuga acacgcccga ccucaagauc      960

aagggggacu ucauccggac caucgaccgc agcaucuaca aggaggagcu gcagcagauc     1020

aucaacaagc agaaggagua ccaccuggag cucaacucca gcaagcugua caaggccugc     1080

cuggaggagc ucuaccccaa gaacgagauc uacaaguccg cccugaagaa caaggacauc     1140

gccuaccugu ucaucaacga cauccucuac uaccagcggc cccugaaggc cccgaaguac     1200

aacaucggca agugcaccua cgagagccgc accuucaaga aggaggacaa gaagauccgc     1260

gaguacaucc ccgcgguggc caagucccac ccccuguucc aggaguuccg gcucuggcag     1320

uggaucaaga accugaagau cuacgagaag cugagcagcg acaacugcac ggacuucuuc     1380

cucaccaacg agcaggacuu cgagaaccug uucgaguucc uguggaacaa gaaggagauc     1440

gaccacaagg gcauccugga guaccucgug aagaccaagu uccccgaccu gaagccgaag     1500

caggucaaga ccaaggugaa ggaguaccgc uggaacuacg uguacgacca cgagaaggac     1560

gaguccaaga cguaccccug cggggagacc uacuuccugc ucaagaacaa gcuggacaag     1620

aucuacaacg cccaggacga cuuccuggac aaggagcggc ucuacaaccu guggcacauc     1680

aucuacagcg ucaccgacaa gaagcaguac gagaaggccc uggccaaguu cgcgcucaag     1740

aaccaccugg acaccaaguc cuucgugaac acguucaagc gcaucccgcc cuucaagaac     1800

gacuacgcca ucuacagccu gaaggccauc aagaagcucc ugccccugau gcgggugggc     1860

aaguacugga acuacgacga caucgacccc aucaccaagc agaagaucca gugcaucauc     1920

accggggagg agaacgacac caucuccacg cucguccgcg agaagaccaa ccacuucagc     1980

aacgagaacg acuucaagaa ccugccccug uggcucgccg gcuacaucgu guacaacucc     2040

cacaacgaga gcuccgagau cgagaagugg aaguccccgc aggacaucga caccuuccug     2100

aagaaguuca agcagcacgc ccugcggaac cccaucgugg agcaggucgu gaccgagacg     2160

cuccgcgugg uccgggacau cuggagcaag aaggggcagg gcaagaagga cuucuucaac     2220

gagauccaca ucgagcuggg caaggaccug aagaaccccg uggacaagcg ccggaagcuc     2280

agcuccacca acaccaagaa cgagagcacc aacacccgca ucaaggcgcu gcugaucgag     2340

cuguucacgc agggggugga gaacgucaag cccaacuccc ccaagcacca ggagauccuc     2400

aagaucuucg aggagggcgc ccugaacagc gggaucgaga ucgaggagga caucaucaag     2460

aucuccaaca ccgaccagcc gaccaagaag cagcucgagc uguacauccu guggcucgag     2520

cacaaguaca agagccccua caccggccag uccauccccc ugagcaagcu guucacgccg     2580

aacuacgaga ucgagcacau caucccccgg agcaaguacu acgacgacaa ccucuccaac     2640

aaggugaucu gcgagaccga gaucaaccgc cugaagaaca accagcuggg guacgaguuc     2700

aucaacaacc acgagauccg ggaggucacc cucgaggagg gcaagaccau ccgccugcug     2760

agccccgagc aguacuggaa cuucgugacg accuacuacg ccaagaacaa ggccaagcgg     2820

gagaaccucc ugcugaagga gcuccccgug cagauggucg agaagcagcu gaacgacacc     2880

cgcuacgugu ccaaguacgu gaugcagcug cucagcaaga ucguccggaa ggaggacggc     2940

accgacaacg ggaucaacuc cacgaacauc cugagcaucu ccggcaagau gacccgcgcc     3000

cugaagaacg acugggaccu caacgacgug uggaacgacc ugcugcucua ccgcuucgag     3060

cggcugaaca ccaucaccaa gacgaaccug uucaccaccu acagcgagca gaagcagaag     3120

cgcaugccga ucgugcccga ccaguacgcg aagggguucc acaagaagcg gcucgaccac     3180

cgccaccacg cccuggacgc caucgucauc gccugcauca cccggaacca caucaacuac     3240

augaacaacc agaacaucca gggcgagaag cugaagaaga acgcgaucga gaaggagaac     3300

uaccgcgaga uccuccagaa gucccggcug gacauccgcg cccagcugug cgagaagaag     3360

uacaacgaca acgaccccac gaaguacaac uggguguuca ucaagcccug ggccgacuuc     3420

accgagaaca ccaaggacau ccucgagagc aucaucccgu ccuucaagca gaaccugcgg     3480

aucaucaaca agaccgugaa cuacuacgag aaguacaacg agaacgggaa gaaggucaag     3540

gugcgccaga ucaagggcga caacugggcc auccggaagc cccugcacaa ggagacgauc     3600

agcggcaagg ugcaccucuc cuacaagacc cccgccaagg ggaagauccu gaccgcgacc     3660

cgcaaggucc uggacagcuc cauggacuuc aagagcaucg agaagaucac ggacaccggc     3720

auccagaaga uccucaagaa cuaccuggag uacaagaagu cccccgagcu ggccuucagc     3780

ccggagggga ucgaggagcu caacgccacc aucaccaagu acaacaacgg cuuccaccac     3840

aagcccaucu acaaggugcg gaucuacgag gagggcguga aguucccccu ggggaacaag     3900

ggcaacaaga aguccaagua cgucgaggcc gagaagggga cgaaccuguu cuucgccauc     3960

uacaaggacc aggagaacaa ccgcuacuuc gagaccaucc ccuucaacga cgugcucgag     4020

acccugaaga agggcaugag cgcggugccg gagaagaacc aggaggagaa cacccugguc     4080

uucgcccuca gcccccagga ccugguguuc gugcccacgc uggacgagau cgagaacccc     4140

gacaucgucg acuucaagaa ccucaccaag gaccagacca accggaucua caagauggug     4200

uccagcuccg ggaagcagug cuucuucauc aaggccgagg uggccaccag caucgucaac     4260

aagauggagu ucuccaccag caacaagaug gagaacucca ucgagggcau ccagaucaag     4320

agcaacugcu ggaagcugaa gcuggaccgc cucggcacga ucacccgggu gaucaaguga     4380


<210>  2227
<211>  4383
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2227
augaccaagu ucaaccuggg ccucgacauc gggaccaaca gcaucggcug ggcccuggug       60

aacgaggacu gcgagaacaa gaaggggaag auccuggccg cgggcucccg caucaucccc      120

augacgcagg acauccucga caaguucggg agcggcgccg ccaccgagac ccagaccgcc      180

cugcggacgg acuaccgcuc caagcggaag cugauccagc gcuuccuccu gcggcgcgag      240

cggcugcacc gcguccucaa cgugcuggac uuccugccga agcacuacgc cgagagcauc      300

gacuucgaga aguaccucgg caaguuccug cccaacaccg agcccaagcu gguguacaac      360

cagaaccagu ucaucuucca gaacuccuuc uucgagaugc ucaaggaguu ccaggagaag      420

caccccgagc uguuccugaa caaccagaag aucccguacg acuggaccau cuacuaccuc      480

cggaagaagg cgcugagcca gaagaucgag aaggaggagc uggccuggcu ccugcugcag      540

uucaaccaga agcgcgggua caaccagcuc cgggacgagg aggaggagaa cacccccgac      600

aagcuggucg aguucuacuc ccugaagauc aucaagguga cggccgacga ggccaaggag      660

ggcaucaaca agcgcuggua cagcaucaac cucgagaacg gguggaucua ccggcgcgag      720

uccaagacgc cccuguucaa cugggagggc aagaccaaga acuucaucgu gaccaccgac      780

cugaaccagg acgggagcau caagaaggac aaggagggca aggagaagcg guccuuccgc      840

gcgcccagcg agggcgacug gacccuccug aagaagaaga ccgagaagga gaucuacgag      900

uccgggaaga cccugggcac guacaucuac gacgcccucc ugaacaaccc ggaccugaag      960

aucaaggggg cccucaucaa gaccaucgac cggagcaucu acaaggagga gcugcagcag     1020

aucaucaaca agcagaagga guaccacucc gagcugaaca acagcaagcu cuacaaggcc     1080

ugccuggagg agcuguaccc caagaacgag aucuacaaga acgagcucaa gaacaaggac     1140

aucgccuacc uguucaucaa cgacauccug uacuaccagc gcccccucaa ggcgcccaag     1200

uacaacaucg gcaagugcuc cuacgagagc cggaccuuca agaaggagga caagaagauc     1260

accgaguuca ucccggccgu cgccaagucc cacccccugu uccaggaguu ccgccugugg     1320

caguggauca agaaccucaa gaucuacgag aagcugacga acgacaacua caccgacuuc     1380

uuccugacca acgagcggga caucgagaag cuguucgacu uccucuggaa caagaaggag     1440

aucgaccaca agggcauccu ggaguaccug aucaagacca aguuccccga ccucaagccc     1500

aagcagguca agagcaaggu gaaggaguac cgcuggaacu acaucuacga ccacgagaag     1560

gacgagagca agaacuaccc gugcggggag acguacuucc ugcugaagaa caagcucgac     1620

aagaucaccu ccauccagga cgacuuccug aacaagaagc gccuggagaa ccucuggcac     1680

aucaucuaca gcgugaccga caagaagcag uacgagaagg cccuggccaa guucgcgcug     1740

aagaaccagc ucgacaccaa guccuucgug aacacguuca agcggauccc cgccuucaag     1800

aacgacuacg ccaucuacag ccugaaggcc aucaagaagc ugcucccccu gaugcgcgug     1860

ggcaaguacu ggaacuacga cgacaucgac cccaucacca agcagaagau ccagugcauc     1920

aucaccgggg aggagaacga caccaucucc acgcuggucc gggagaagac caaccacuuc     1980

agcaacgaga acgacuucaa gaaccucucc cuguggcugg ccggcuacau cguguacaac     2040

agccacaacg aguccagcga gaucgagaag uggaaguccc cgcaggacau cgacaccuuc     2100

cucaagaagu ucaagcagca cgcgcugcgc aaccccaucg uggagcaggu cgugaccgag     2160

acgcugcggg ugguccgcga caucuggcuc aagaaggggc agggcaagaa ggacuucuuc     2220

aacgagaucc acaucgagcu gggcaaggac cugaagaacc ccguggacaa gcggcgcaag     2280

aucagcucca ccaacagcaa gaacgagacc accaacaucc ggaucaaggc ccuccugaag     2340

gagcugcuca aggaggggau cgagaacauc aagcccaacu ccccgaagca gcaggagauc     2400

cugaagaucu ucgaggaggg cauccugaac agcgggaucg acaucgagga ggagaucauc     2460

aagaucuccc agaucgccca gcccaccaag aagcagcucc aguacuaccg caacuggcug     2520

gagcagaagu acaucagccc cuacacgggc caguucaucc cccuguccaa gcuguucacc     2580

agcaaguacg agaucgagca caucaucccg cggagcaagu acuacgacga cucccucagc     2640

aacaagguga ucugcgagac cgagaucaac cgccugaaga acaaccagcu cggguacgag     2700

uucaucaaca accacgagau ccgggagauc acccuggagg agggcaagac gauccgccug     2760

cucuccccca agcaguaccu ggacuucguc accaccuacu acggcaagaa caaggccaag     2820

cgggagaacc ugcuccugaa ggagaucccc auccagauga ucgagaacca gcugaacgac     2880

acccgcuacg ugagcaagua cgugaugcag cuccugucca agaucguccg gaaggaggac     2940

gggacggaca acggcaccaa cagcaucaac auccuguccg ugagcgggaa gaucacccgc     3000

guccucaaga ccgacugggg ccugaacgac guguggaacg accugcucug cuaccgguuc     3060

gagcgccuga acaagaucac gaacaccaac cuguucacca ccuacuccga gcagaagcag     3120

aagaucaucc cgaucgugcc cgaccaguac gccaaggggu uccacaagaa gaagcucgac     3180

caccgccacc acgcgcugga cgccauccug aucgccugca ucacgcggaa ccacaucaac     3240

uacaugaaca accagaacau ccagggcgag aagcucaaga acuucaagau cgacaagcac     3300

aacuaccacg agaucuucca gaagucccgc cuggacaugc gggcccagcu gugcgagaag     3360

aaguacaacg accacgacag caccaaguac accugggucu ucaccaagcc cugggcggac     3420

uucacggaga acaucaagga cacccucgag uucaucaucc ccagcuucaa gcagaaccug     3480

cgcaucauca acaagaccgu gaacuacuac gagaaguaca acgagaacgg caagaaggug     3540

aagguccggc agaccaaggg ggacaacugg gccauccgca agccgcugca caaggagacg     3600

aucuccggca aggugcaccu ccuguacaag acccccgcca aggggaagau ccugaccgcc     3660

acccggaagg ugcucgacag cuccauggac uucaagagca ucgagaagau cacggacacc     3720

ggcauccaga agauccugaa gaacuaccug aaguacaagg agucccccga gcucgccuuc     3780

agccccgagg gcaucgagga gcugaacauc accaucgcga aguacaacga cggguucuac     3840

cacaagccga ucuacaaggu ccgcaucuac gaggagggcg ugaaguuccc ccuggggaac     3900

cggggcaaca agaagaacaa guacguggag gccgagaagg ggaccaaccu cuucuucgcc     3960

aucuacaagg accaggagaa caaccgcuac uucgagacga uccccuucaa cgacguccug     4020

gagacccuga agaagggcau gagcgccgug cccgagaaga accaggagaa gaacacccuc     4080

guguucgccc uguccccgca ggaccugguc uucgugccca cccucgacga gaucgagaac     4140

cccgacaucg uggacuuccg gaaccugacg aaggaccaga ccaaccgcau cuacaagaug     4200

gucagcucca gcggcaagca gugcuucuuc aucaaggcgg agguggccac cuccaucgug     4260

aacaagaugg aguucaccac cagcaacaag auggagaacu ccaucgaggg gauccagauc     4320

aagagcaacu gcuggaagcu gaagcucgac cggcugggca cgaucacccg cgucaucaag     4380

uga                                                                   4383


<210>  2228
<211>  4389
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2228
augaccaaga acauccuggg ccucgaccug gggaccaaca gcaucggcug ggcccugauc       60

aagcagaacu ucgagaacaa gcagggggag auccucggca uggggucccg caucaucccc      120

augagccagg acauccuggg cgacuucggc aaggggaacu ccgugagcca gacggccgag      180

cggaccaagu accgcuccgu ccggcgccug cgggagcgcu uccuccugcg gcgcgagcgg      240

cugcaccgcg ugcucaaccu gcugaacuuc cucccgcagc acuacgcgag ccagaucgac      300

uucgagaagc gguucggcaa guucaaggug gagaccgagc ccaagcuggc cuggaagaac      360

aucgacgggc aguucuccuu ccuguuccag accagcuuca acgagaugcu cgaggacuuc      420

cgcgucaacg gccaggacgu gaagaucccc uacgacugga cgaucuacua ccugcggaag      480

aaggcccugu ccaagaagau cgagaaggag gagcucgccu ggauccugcu gaacuucaac      540

cagaagcgcg gguacuacca gcuccggggc gaggaggagg aggagaaccc caacaagcug      600

guggaguucu acagccugaa ggucguggac guggucgccg acgagccgca gaagggcaag      660

uucgacaucu gguacucccu cauccuggag aacggguggg uguaccgccg gagcuccaag      720

aucccccugu ucgacuggaa ggacaagacc cgcgacuuca ucgugaccac cgaccucaac      780

gacgacggca gcaucaagaa ggacaaggac gggaacgaga agcgguccuu ccgcgcgccc      840

agcgagaacg acuggacgcu ggucaagaag aagaccgagc aggagaucga gcagucccgg      900

aagaccgugg gcaccuacau cuacgaggcc cugcucgaca accccaagca gaagaucaag      960

ggcaagcugg ugcgcacgau cgagcgcaag uucuacaagg aggagcugcg gcagauccuc     1020

gagaagcaga aggaguucca ccaggagcug cagagcgacg accuguacaa cgacugcguc     1080

cgcgagcucu accggaacaa cgaggcccac cagcugaccc uguccaagaa ggacuucgug     1140

caccuccuga uggacgaccu gaucuucuac cagcgcccgc uccggagcca gaaguccagc     1200

gugagcaacu gcacccugga guuccgcaag uacaaggacg agaacggggc cgagcacacc     1260

caguaccuga aggcgauccc caaguccaac cccuacuacc aggaguuccg gcucuggcag     1320

uggaucuaca accugaacau cuaccgccgg gaggacgagc agaaccccau cacgggcgag     1380

uuccugagca ccaccaagga guucgagaac cucuucgagu uccugaacac ccgcaaggag     1440

gucgaccaga aggcccugcu gaagcacuuc aagcucaacg acaagacgca ccgguggaac     1500

uucguggagg acaagaagua cccgugcaac gagaccaaga ccaugaucuc cagccgccug     1560

gagaaggugg agaacaucuc cgacgacuuc cugacccggg agaucgagca gaagaucugg     1620

cacaucaucu acagcgucaa cgacaagauc gaguacgaga aggcccucaa guccuucgcc     1680

aagaagcacc accuggacga gagcuccuuc uucgaggccu uccgcaaguu cccgcccuuc     1740

aagagcgagu acggguccuu cagcgagaag gcggugaaga agcugcuccc ccugaugcgg     1800

cugggcaagu acuggaacua cgccgacauc gacaagucca gcaaggagcg cauccagaag     1860

aucaucuccg gggaguacga cgagaacauc aaggaccggg ugcgcgagaa guccguccac     1920

cucacgaccg agaacgacuu ccagggccug cagcuguggc ucgcccagua caucguguac     1980

ggccggcaca gcgaggccag caugaucggg aaguggaaca gcgccgacga ccuggaggag     2040

uuccugaaga acuucaagca gcacucccuc cgcaacccca ucgucgagca ggucaucacc     2100

gagacccugc gcguggugaa ggacaucugg cugaaguacg gcaacggggc gaaggacuuc     2160

uucaacgaga uccacaucga gcucggccgg gagaugaagc ugcccgccaa ggagcgcgag     2220

gaccugacgc ggaagaucac cgagaacgag aacaccaacc uccgcaucaa ggcccugcug     2280

gccgagauga ugaacgacag cuccguggag aacgugcggc ccuucagccc caugcagcag     2340

gagauccuca agaucuacga ggacgggguc cugaacuccg gcaucgagau cgaggacgag     2400

uaccugaaga ucagcaagac cgcccagccg ucccccagcg accucaagcg cuacaagcug     2460

uggcuggagc agaaguacaa gucccccuac acgggccaga ucaucccccu caacaagcug     2520

uucaccccgg aguacgagau cgagcacauc aucccccaga gccgguacuu cgacgacucc     2580

uucagcaaca agaucaucug cgaguccgcg gugaacaagc ugaaggacaa cuacaucggg     2640

cucggcuuca ucaagcaguu cgggggcacc aucaucgagc ugggguucgg caagagcgug     2700

aaggucuucg agaccgagga guacgaggac uucgugaaga agcacuacgc caacaaccgc     2760

uccaagcgga acaagcugcu ccuggaggac auccccgaga agaugaucga gcgccagauc     2820

aacgacacgc ggcacaucag caaguacauc uccggcgugc ugagcaacau cguccgcauc     2880

gacgacgacc aggccaagaa gaucaucgag gacgccgaga uccucgcccg gaaggagauc     2940

gacaaggaca aggcgaacga gauccgccgg aaggccaaga gccuggccac cgacgacggg     3000

aucaacucca agaacaucgu gcccggcaac gggaagauca ccacccagcu gaagcaggac     3060

uggggccuca acgacgugug gaacgaccug auccugccgc gcuucgagcg gaugaaccag     3120

cucaccaaca gcaaggacuu cacggucugg aacgagaacc accagaagua ccugcccacc     3180

gugcccaucg aguucuccaa gggcuucagc aagaagcgca ucgaccaccg gcaccacgcc     3240

cuggacgcgc ucgugaucgc cugcgccacc aaggaccacg ucaaccugcu gaacaaccag     3300

uccgccaaga gcgacaccaa gcgcuacgac cucaagaaga agcugaugaa guucgagaag     3360

gugguguacc accacacgca gaccgggaac aagaucgagc gggagauccc gaagcaguuc     3420

cugaagcccu gggagaacuu caccuucgac gccaaguaca accucgaguc caucaucguc     3480

agcuucaagc agaaccugcg cgugaucaac aaggcgacca acuacuacga gaaguacgug     3540

gagaaggacg gcacgaagaa caaggggcgc gucgagcaga ccggcaccaa cugggccauc     3600

cggaagccca ugcacaagga caccgugucc gggaagguga accugcccug ggucaaggug     3660

ccgaagggca agauccucac ggccacccgc aagauccugg acaccagcuu cgaccugaag     3720

uccaucggca gcaucaccga cacggggauc cagaagaucc uccggaacua ccuggaguuc     3780

aagggcuccc ccgagcuggc cuucagcccc gaggggaucg aggaccucaa caagaacauc     3840

gagaaguaca acgacggcaa gccccaccag ccgaucaaca aggugcgcgu cuucgagcug     3900

ggguccaagu uccagguggg ccagaccggc aacaagaagg acaaguacgu ggaggccgcg     3960

aaggggacca accuguucuu cgccgucuac gaggacaaga acggcaagcg gagcuacgag     4020

accauccccc ucaacgaggu gaucgagcgc cagaagcagg ggcuguccgu ggucgaccug     4080

aagggcacga acgacuucua ccucugcccc aacgaccugg uguacauccc cagcgacgac     4140

gagcuggaga acaucaacaa caucgacuuc aagaacaucg ccaaggagaa gaacgagcgg     4200

aucuacaagg uggucuccuu cagcgggagc cagaucuucu ucgugcgcca ggacaucgcc     4260

accuccaucg ugaacaaggc cgagcucagc acccugaaca agauggagcg ggcgauggac     4320

ggcuccauga ucaaggagag cugcgucaag cugaacgugg accgccucgg caacaucucc     4380

aaggccuga                                                             4389


<210>  2229
<211>  4401
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2229
augaccaaga acauccuggg ccucgaccug gggaccaaca gcaucggcug ggcccugauc       60

aagcaggacu ucgagaacaa gcagggggag auccucggca uggggucccg caucaucccc      120

augagccagg acgugcuggg cgaguucggc aaggggaacu ccgucagcca gacggccgcg      180

cggaccgacu accgcggcgu gcggcgccug cggaagcgcu uccuccugcg gcgcgagcgg      240

cugcaccgcg ugcucaacgu ccugaacuuc cugccggugc acuacgccuc ccagaucgac      300

uucgagaagc gguucgggaa guucaaggag gacaccgagc ccaagcucgc cuacaacaag      360

gagggcuuca ucuucaagaa cagcuucgag gagauggugg ccgacuuccg caagcaccag      420

ccccagcugc uggagaacga caagaagauc cccuacgacu ggaccaucua cuaccuccgg      480

aagaaggccc uguccaagaa gaucgagaag gaggagcugg cguggauccu ccugaacuuc      540

aaccagaagc gcggguacua ccagcugcgg ggcgaggagg aggaggagaa cccgaacaag      600

cucgucgagu ucuacagccu gaagaucacg gacgugcugg ccgacgagcc ccagaagggc      660

aaguccgaca ucugguacag ccucauccug gagaacgggu ggaucuaccg ccgguccagc      720

aagacgcccc uguucgacug gaaggagaag acccgcgacu ucaucgugac caccgaccuc      780

aacgacgacg gcuccaucaa gaccgacaag gacgggaacg agaagcggag cuuccgcgcc      840

cccuccgaga acgacuggac ccuggucaag aagaagaccg agcaggagau cgacaagagc      900

cagaagacgg ugggcaccua caucuacgag acccugcucc agaacccgaa gcagaagauc      960

aagggcaagc uggugugcac caucgagcgg aaguucuaca aggaggagcu gaagcagauc     1020

cucgagaagc agaaggaguu ccaccaggag cugcagaacg acgaccugua cuccgacugc     1080

guccacgagc ucuaccgcac gaacgaggcc caccagcuga cccugagcaa gaaggagauc     1140

gugaaccucu ucauggagga ccugaucuuc uaccagcgcc cccugcgguc ccagaagacc     1200

agcaucagca acugcacccu cgaguuccgc aaguacaagg acgagaacgg gguggagcac     1260

acgcaguucc ugaaggcgau ccccaagucc aaccccuacu accaggaguu ccggaucugg     1320

caguggaucu ucaaccugaa caucuacaag cgcgacgacg acagcaacgu caccaaggag     1380

uuccucaaca ccaccgagga cuucgagaac cuguucgagu uccugaacaa ccggaaggag     1440

guggagcaga aggcccuccu gaagcacuuc aagcugaacg agaagcugca ccgcuggaac     1500

uucguggagg acaagaagua cccgugcaac gagacgaaga ccaugaucuc cacccggcuc     1560

gacaaggucg agaacaucag caaggacuuc uucacccgcg agaucgagca gaagaucugg     1620

cacaucaucu acuccgugaa cgacaagauc gaguacgaga aggcccugaa guccuucgcc     1680

aucaagcaca accuggacga gaacuccuuc uucgaggccu ucaagaaguu cccgcccuuc     1740

aagagcgagu acggcuccuu cagcgagaag gcgaucaaga agcuccugcc ccugaugcgg     1800

cucgggaagu acuggaacga ggacgagauc cugaaguacu ccgacgacua cuucaagaac     1860

aucgagagcc ugcucggcgu gcugaagcag cgcgacgaga acaucuccga cgccgaccgg     1920

gagaacuaca acaagacgau caacaacaag cugcgcgccg agcucgagaa cuuccagaac     1980

gccgagaucg agagcuucca ggggcugcgg cugcacaucg cccaguacau ccucuacggc     2040

aagcacuccg agggcgacaa caagaaguuc aacagcgcgg acgaccugga gaaguuccug     2100

aaggaguuca agcagcacuc ccuccgcaac cccaucgucg agcaggucau caccgagacc     2160

cugcgggugg ugaaggacau cuggaccaag uacgggaacg gcgccaagga cuucuucgac     2220

gagauccaca ucgagcuggg gcgcgacaug aagaagacgc gggacgagcg cgccgacgcc     2280

accaagauca ucaccgagaa cgagaacacc aaccuccgga ucaaggcccu gcuggcggag     2340

augaugaacg acguggagau ggagaacguc cgccccuaca gccccaugca gcaggagauc     2400

cucaagaucu acgaggacgg cauccugaag uccgacaucg agaucgagga cgacauccug     2460

aagaucagca agacggccca gcccagcucc agcgaccuca agcgcuacaa gcuguggcug     2520

gagcagaagu acaagucccc guacaccggg cagaucaucc cccucaacaa gcuguucacc     2580

cccgaguacg agaucgagca caucaucccc cagagccggu acuucgacga cuccuucagc     2640

aacaagauca ucugcgaguc cgccgugaac aagcugaagg acaacuacau cggccucggc     2700

uucaucaagc aguucggggg caccaucgug gagugcgggu ucggcaagaa cgucaaggug     2760

uucgaggccg aggaguacga ggacuucgug aagaagcacu acgccaacaa ccgcgggaag     2820

cggaacaagc ugcugcucga ggagaucccg gagaagauga ucgagcgcca gaugaacgac     2880

acccggcaca ucagcaagua caucuccggc guccugagca acaucgugcg cguggaggac     2940

ggcuccgacg agggggucaa cagcaagaac aucgugcccg gcaacgggaa gaucacgacc     3000

cagcugaagc aggacugggg ccucaacgac guguggaacg agcugauccu gccccgguuc     3060

gagcgcauga accagcucac caacuccacc gacuucacgg cguggaacga gaaccaccag     3120

aaguuccugc ccaccguccc gaucgaguuc agcaagggcu ucuccaagaa gcggaucgac     3180

caccgccacc acgcccugga cgcccucauc aucgccugcg cgaccaagga ccacgugaac     3240

cugcugaaca accagagcgc caaguccgac accaagcggu acgaccucaa gaagaagcug     3300

augaaguucg agaagguggu cuacaaccac ccccagacgg gggaccgcau cgagcgggag     3360

gugcccaagc aguuccugaa gccgugggac aacuucaccu ucgaggccaa gaacaaccuc     3420

gacaagauca ucgugagcuu caagcagaac cugcgcguca ucaacaaggc caccaacuac     3480

uacgagaagu ucguggagaa ggacggcgug aagaccaagg agcgggucga gcagaagggg     3540

acgaacuggg ccauccgcaa gagccugcac gaggagaccg uguccggcaa gaucgaccuc     3600

cccugggugg agaccaacaa cggggaguuc aucaccgcga cgcgcaagag ccuggacgcc     3660

uccuucaacc uggagaagau caagaagauc accgacaccg gcauccagaa gauccuccgg     3720

aacuaccuga aggccaagga cgacaacgcc gagcuggccu ucagccccga gggcaucgag     3780

gaccucaaca agaacaucga gaaguacaac gacgggaagu cccaccagcc caucguccgg     3840

gugcgcgugu acgagaaggg caaggggcgg uucgcgcugg gccagaccgg gaacaacauc     3900

gacaaguacg uccagggcag cccgaaccug uucuucgcca ucuacaagga cgagaacggc     3960

aagaaguccu acgagagcau ccgccucgac aucgugaucg agcgcaugaa gcaggggcug     4020

caggccgugc ccgagacgaa ccccaacggc aucucccugu accagaaccu cagcccccug     4080

gaccuggucu acaucccgac cgaggacgag aucgagagcc cccaccucau cgacuucacc     4140

auccugaaca agacccggcu ggagcgcauc uacaacguga acgacuucag cggguacacg     4200

aucuacuucu cccagaacag cuucgccaag aacaucuacc ccaaggagau ggacaugacc     4260

uggaacgaga agaagcagaa gcccuccggc agcuucgacu ccaagaccgc cagcuacaac     4320

aagaagucca ucaaggacau cuucaucaag cucaagaucg accggcuggg gaacaucagc     4380

aaggugagca acacccugug a                                               4401


<210>  2230
<211>  4401
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2230
augaagaaca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaag       60

gaggacuucg agaacaagca gggggagauc cucggccugg ggucccgcau cauccccaug      120

agccaggaca uccugggcga guucggccgg gggaacucca ucagccagac cgccgaccgc      180

accggcuacc gcgggguccg gcggcuccgc gagcggcacc uccugcgccg ggagcgccuc      240

caccgggugc ugaacauccu gggcuuccuc ccgaaccacu acgcgaagga gaucgacuuc      300

accaagcgcc uggggaaguu caucaaccac gccgagccca agcuguccuu cgacaaggag      360

uucaucuuca aggacagcuu caacgagaug cucaaggacu uccagcagaa ccagcccggc      420

uucaucauca acaagcacgg cgaggaggcc cuggugcccu acgacuggac caucuacuac      480

cugcggaaga aggcccucac ccagaagauc gacaaggagg agcuggccug gauccugcuc      540

aacuucaacc agaagcgcgg guacuaccag cugcggggcg aggaggagga ggagaacacg      600

aacaagcugg ucgaguucua cucccucaag aucaucgacg ugaucgcgga caacgaggug      660

aacaagaagg gggaggucug guacagccug caccuggaga acggcuggau cuaccgccgg      720

uccagcaaga ucucccucgu ggacuggaag gacaaggugc gcgacuucau cgucaccacc      780

gacgugaacg acgacggcag cgagaagcug gacaaggacg ggguggucaa gcgguccuuc      840

cgcgccccga gcgccgacga cuggacccug cucaagaaga agacggagca ggacaucgac      900

aacuccaaca agaccguggg cgccuacauc uacgagaacc ugcugcucaa ccccaagcag      960

aagaucaagg ggaagcuggu gcggaccauc gagcgcaagu ucuacaagga cgagcugaag     1020

cagauccucg agaagcagaa ggaguuccac caggagcugc agaacgagca gcugcuccag     1080

gacugcgucc gcgagcugua caaguacaac gagcagcacc agcagaugcu ggagagcaag     1140

gacuucgugc accucuuccu gaacgacauc aucuucuacc agcggccccu gcgcucccag     1200

aagucccuca ucuccaacug cacccuggag aagcggguga gccgcgacgg cgucguguuc     1260

cccaucaagg ugaucagcaa guccaacccg uacuaccagg aguuccggcu gcuccagugg     1320

cugcagaacc uggcgaucua ccgcaaggac gacgacguca acgugacgca ggaguuccug     1380

aacagcaucg aguccuggga gaaccuccug caguuccuga acgccaagaa ggagaucaag     1440

caggagcagc ucaucaaguu ccugcuggag cagaaggggc ucaagggcaa cagccugaag     1500

accgccaucc ugaccuaccg guggaacuac gugaccgaca aggucuaccc caugaacgag     1560

acgcgcuccc ucauccagga caagcugaag aagguggaga acgucgccac cgacuuccug     1620

agcuucgaca acgagauggc ccucuggcac aucaucuacu ccgugaacga caagaucgag     1680

uacgagaagg cgcuggccaa guucgccagc aagcacaacc uggacgagau cuccuucgug     1740

gagagcuuca agaaguuccc gcccuucaag uccgacuacg gcagcuucuc cgagaaggcc     1800

aucaagaagc uccugccccu gaugcgguuc gggagcgucu ggaacaagaa caacaucgug     1860

cccuccgugc ucgaccgcau cgacaagauc cugaccggcg aguacgacga gaagaucaag     1920

auccgggucc gcgagaaggc cgagcaguuc cagcugaacc agaucgagga cuuccagaac     1980

cucccccugu ggcuggcgca guacguggug uacgaccggc acagcgaggc cgagaucgcc     2040

gggaagugga acuccgucaa ggaccucgag aacuaccuga aggaguucaa gcagcacagc     2100

cugcgcaacc ccaucgugga gcagaucauc accgagacgc uccggguggu caaggacauc     2160

uggcagcagu acggcaacgg ggugcagaac uucuucgacg agauccacau cgagcugggc     2220

cgcgagauga agaacaccgc cgacgagcgg aagcgcauca ccaacuccgu gaccgagaac     2280

gagaacacga accugcggau caaggcccuc cuggucgagc ugaaggagga caccaacauc     2340

gagaacgugc gcccgcacag ccccaugcag caggagaucc ucaagaucua cgaggagggc     2400

gugcugaacg cggucgagaa caucgacgag gacauccuga agaucagcaa gaccgcccag     2460

cccuccaaga gcgagcucau ccgcuacaag cuguggcugg agcagaagua ccaguccccc     2520

uacaccgggg agaugauccc gcucagccgg cuguucacgu ccgacuacga gaucgagcac     2580

gugauccccc agagccgcua cuucgacgac ucccugagca acaaggugau cugcgaggcc     2640

gccgucaaca agcggaagga caaccagcuc ggccuggagu ucaucaagaa ccaccacucc     2700

gagaagaucg agcuggggaa cggccgcacc gcccaggugc ucgaggugga ggcguacgag     2760

agcuucguca agaagaacua cgacaagaac cgggggaagc gcaacaagcu gcugcucgag     2820

gacgugcccg agaagaugau cgagcggcag cugaacgaca cccgcuacau cuccaaguuc     2880

gugacccacc uccugagcaa caucguccgg aaggacgagg ugaacuccaa ggacgacggc     2940

aucaacagca agaacaucau ccccggcaac gggaagauca cgucccagcu gaagcaggac     3000

uggggccuga acgacgugug gaacgagcuc auccugccgc gcuucgagcg gaugaacaac     3060

cugaccaaca gcaccgacuu caccuccacg aacaccaacg ggaagaucau ccccaccguc     3120

ccccucgagu acagcaaggg cuucagcaag aagcgcaucg accaccggca ccacgcccug     3180

gacgcccugg ugaucgcccu cgcgacccgc gaccacguga accugcugaa caacgagucc     3240

gcccucccca agaaggaccg gaccaaggag gagcagaagu ccuuccgcuu cgaccugcag     3300

gucaaccugc ggaacaagga gaaguacuuc aacgugaagg agggccgcga gaaggagaag     3360

uucacgaccu ucaagaagcc guggaacacc cucacccagg acacgcggga cgcccuggag     3420

aacauggugg ucagcuucaa gcagaaccug cgcgugauca acaaggccac caacuacuac     3480

gagagcuaca ccgacgagaa cgggaaccuc cggcuggaca aguacggcaa ccccaagaag     3540

gagcugaucu cccagaaggg gaccaacugg gccauccgca agagccucca caaggagacg     3600

guguccggcc agauccaccu ggaccgcguc aaggugacca aggggaagau cgugaccgcg     3660

acccgcaaga gccuggacuc cagcuucaac gagaagucca ucgagagcau cacggacacc     3720

ggcauccaga agauccuccg gaacuaccug aaggccaagg acaacaagcc cgacgucgcc     3780

uucuccccgg agggcaucga ggagcugaac aagagcaucu ccguguacaa cgaggggaag     3840

aaccaccagc ccaucaucaa ggugcggauc uucgagcagg gcagcaaguu ccccgucggg     3900

gagaccggca acaagaucgc caaguucgug gaggccgcga aggggaccaa ccucuucuuc     3960

ggcaucuacc aggacaagac gggcaagcgc uccuacgaca ccaucccccu gaaccuggug     4020

aucgagcggc ucaagcaggg gcugagcccg guccccgaga ccaacgagaa gggccaccag     4080

cugcuguucc accugucccc caacgaccug guguacgugc ccaccaucga cgacaacgag     4140

cagcucgaca gcaaccgcgu cuacaaguuc acggacagcu ccgacacgac cugcaacuuc     4200

aucccgagca acaucuccgc caucaucuuc aacaucaaca gcaaggagca ggagaagcgg     4260

gggaucaacu accccaucca gaacgagcug ggcgugggga gcccccagag caagaaccag     4320

aaguccuucg acggcuucau gaucaaggag gucugcauca agcugaaggu ggaccgccuc     4380

ggcaacauca gcaagaccug a                                               4401


<210>  2231
<211>  4416
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2231
augugcaagu acuucuacau caagaacuuc aucaccuucg ugcugagcaa guccaucauc       60

cccaugaagc acauccucgg ccuggaccug gggagcaacu ccaucggcug ggccuucguc      120

cagcaggacu ucgagaacaa gcaggggaag aucaucgcca ccggcagccg caucaucccg      180

auggaccagg ggauccucgg cgacuucgag cggggcaaca cgguguccca gaccgcggag      240

cgcaccaccu accggagcac gcgccggcug cgcgagcggc accuccuccg ccgggagcgc      300

cugcaccggg ugcugcacau ccucggguuc cugccgcccc acuacgacgc ccagaucgac      360

uucaccaagc gcuacggcaa guucaucgac aacgccgagc ccaagaucgc cuacaacaac      420

gggaacuuca ucuucaugaa cuccuucaac gagaugaucg aggacuucaa gaagcaccag      480

ccccagcugu ucuacaagaa guccaacggc gaggagagca agauccccua cgacuggacc      540

aucuacuacc uccggaagaa ggcccugagc cagaagauca cccagcagga gcuggcgugg      600

cucauccugc acuucaacca gaagcgcggg uacuaccagc ugcggggcga ggaggagacg      660

gagaacccca acaaggaggu cgccuuccac ucccucaagg ugguggacgu cgaggccgag      720

gcccccaaca agaagggcga gaucugguac accauccgcc uggagaacgg guggaucuac      780

cggcgcacca gcaagauccc gcuggacgac uggaagggca agacccggga cuucaucgug      840

acgaccgacc ucaacgacga cgggaccauc aagguggaca aggagggcaa ggagaagcgc      900

uccuuccggg cgcccgacga gaacgacugg aaccugcuga agaagaagac cgagcacgac      960

aucgacuuca gcggcaagac ggucggggcc uacaucuacg accaccuccu gcagaacccc     1020

aaccagaaga ucaagggcaa gcuggugcgc accaucgagc gcaaguacua caaggacgag     1080

cucaaggcca uccuggccaa gcagaucgcc cugcagcccg agcuguucac cgagacccug     1140

cuggcggacu gcauccggga gcucuaccgc aagaacgaga cgcagcagca gaaccugcug     1200

uccaaggacu uccuccaccu guucguggag gacaucaucu ucuaccagcg gccgcugcgc     1260

agcaagaagu ccaccaucgc caacugcacc cucgagaagc ggagcuacau cgacaaggag     1320

accaacgagc gcaaggacgc ccccaucaag gucugcgcca aguccaaccc cuacuaccag     1380

gaguuccggg ugcugcagug gcugcagaac cuccgcaucu acgagaucga gacggaccgg     1440

gaggugaccg ccgaguucau cgagacgccc caggacugcg aggcgcuguu caacuuccug     1500

augagccaga aggagaucga caacaagggg cuccugaagu acuuccuguu cgccaagaac     1560

ccgcagauca aggacaaggc cgccaagagc gagcucaaga aguggcugac caccuaccgc     1620

uggaacuacg ucuacgacgc cgcggacaac uccagcaaga aguaccccau gaacgagacc     1680

ggcuacgagc ugcagcggua ccugcagaag guggaggggc uccccgccaa cuuccugacc     1740

uucgacauca ccuaccagcu guggcaccuc cuguacuccg ugaaggacaa ggucgaguuc     1800

gagaaggccc ugaagaaguu cgccaagaag aagcagcucg acaagacgag cuucguggac     1860

uccuucaaga acuucaagcc cuacccgaac gaguacggca gcuucuccga gaaggccauc     1920

aagaagcugc ugccccucau gcgcuucggc aaccacuggg acuucaaccg gaucgacgug     1980

agcacccaga agcgcaucga cgaccugauc aacggggucg cgaacgagga cauccggacc     2040

aucauccgcg agaaggccga gaaguaccag cugaacuccg agaccugcuu ccaggaccuc     2100

ccccuguggc uggcccagua cgugguguac aaccggcacg ccgaggcgac ggacaucgag     2160

aaguggacca gcgucuccga ccucgaggcc uaccuggagg acuucaagca gcacagccug     2220

cgcaacccca ucguggagca ggucgugacc gagacccucc gggugguccg cgacaucugg     2280

cagcaguacg gcgggggcca ggccaacuuc uucgacgaga uccacaucga gcuggggcgc     2340

gagcugaaga agaccgccga ggagcggaag cagcucuccg acaagaacca ggagaacgag     2400

agcacgaacc ugcgcaucaa gaagcugcuc gccgagcuga aggaggacgc guccauccag     2460

aacgugcggc cguacagccu ggugcagcag gagcuccuga agaucuacga ggagggcguc     2520

uucuccgcca ccgacgaggu ggaggaggac auccagaaga uccgcaagaa ggccgagccc     2580

agcaaggccg accugcagcg guacaagcuc uggcuggagc agaaguacaa gucccccuac     2640

accggcgagg ugaucagccu gaccaagcug uucacggagg aguacgagau cgagcacauc     2700

aucccccaga gccgcuacua cgacgacucc cucagcaaca aggucaucug cgaguccgcc     2760

gugaacaagc ugaagaccaa ccagcucggg cuggaguuca uccagaacca ccacggcgag     2820

aaggugcgga ucaccgcgaa caaggagguc accaucuuca cggaggagca guacaaggag     2880

uucgugaaga agcacuacgc caacaacagc gccaagcagc agaagcugcu ccugaacgag     2940

aucccggaga agaugguggc ccgccagcug aacgacaccc gguacaucuc caaguacauc     3000

agcgagaucc ucuccaagau cguccgcagc gacgagaagg acgagggggu gaacuccaag     3060

aacaucaucc ccugcaccgg caagaucacc acggcccuga agcaggacug ggggcugaac     3120

gacgugugga acaagcucau ccugccccgg uucgagcgca ugaaccagcu gacccagacc     3180

cagcuguuca ccagcuacaa cgaccggcuc cagaagaacc ugccgacggu ccccaucgag     3240

uacucccagg gcuuccagaa gaagcgcauc gaccaccggc accacgcgau ggacgcccuc     3300

gugaucgccu gcgccacccg cgagcacauc aacuacauga acaaccagca cgcgcuggac     3360

aagagcaagg acaagaagga gaagcagcag ucccggaucg accugcgcac cauccucugc     3420

gagaagaaga acaacaacug gcaguucaag aagcccugga gcaccuucac gcaggaggcc     3480

aaggaggccc uggagcacau cguggucucc uucaagcaga accugcgggu gaucaacaag     3540

gccaccaacu acuacgagaa gugggaggag aagaacggcg ugcucgucaa gaagaaggug     3600

aagcaggagg ggaccaacug ggccauccgc aagccccugc acaaggagac cgugagcggc     3660

aagaucgucc uggaccggaa gaucgggaag gacaagaucc ucacggcgac ccgcaaggcc     3720

guggacauca ccuucaccga gaagaucauc uccagcauca cggacaccgg cauccagaag     3780

auccugcuga acuaccucaa guacaagggc agcccggagg uggccuucuc caccgagggg     3840

cuggaggagc ugaacaagaa caucgcccag uacaacaacg gcaagaagca ccagcccauc     3900

uacaaggucc gcaucuucga ggaggggagc aaguuccccc ucggcgagac cggggccaag     3960

gcgacgaagu acguggaggc cgccaagggc accaaccugu ucuucggcgu guaccagggg     4020

aagggcaagc ggaccuacgc caccaucccc cugaacgagg ucaucgagcg ccagaagcag     4080

gggcucccgu ccgugcccga gcggaacgag aagggcgagc cccugcuguu cagccucucc     4140

cccaacgacc ugguguacgu cccgauggag ggggagaucg ccgagacgau cgacuucaac     4200

aaccugagca aggagcagaa ggagcgcauc uacaagaccg uguccuucac cggcaaccag     4260

ugcuucuucg ugcaggaggc ggucgccacc cccaucguga acaagaugga guacagcccc     4320

cucaacaaga uggagcggga cauccugggc accaugauca aggaggugug caucaagcug     4380

aaggucgacc gccucgggaa caucacgaag gccuga                               4416


<210>  2232
<211>  4416
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2232
augggcuaca aggcgccccg caacgacaag ggggagaucg acuacggcgu ggggcuggac       60

cucggcaacg ggagcgucgg cuggguggcc auggacaagg acuaccaccu gauccgggcc      120

cgcggccacg agcugaucgg ggcccggcug uucgacccgg ccaacuccgc cgagaaccgc      180

cgggagagcc gcaccacccg gcgccggcug ucccgccggc gcuggcggcu gcgcaugcuc      240

gacagccugu ucgccuccga gcuggcgaag aucgacccca acuuccucgc ccggcgcaag      300

uacagcuggg ugcaccccaa ggacgagcag aaccacgaga acugguacgg cuccaucauc      360

uucgggagca aggagcagga caagcaguuc uacaaggacu accccacgau cuaccaccuc      420

cggaagaagc ugaccgagga caccgagcgc cacgacaucc gggagaucua cgucgccauc      480

caccacaucg ugaaguaccg cggccacuuc cuccgggagg gggagaucaa cuccgagaac      540

cuguucgacc cggagggcuu cguggagcug aucaaggacg ucaucaagcu caacaacagc      600

uacgacgagu ccacccccau cuucgaggcc agccccgcgg acguguccga ggcccugcug      660

aacgagcaca cgagccgcuc cggccgcauc gaggacaccc ucgacaagcu ggacuucagc      720

gugucccggg ucgacaagca cgagcugaag gggaugcgcg acgccgccaa ggccgugauc      780

ggcgcgaucg uggggaacac cgccgacuuc agcaagaucu ucaaccucga caaccuggac      840

aaggacucca aggacaaccu gaagcucaag uucgccgaca gcaacuucga cgagaaguac      900

ggcggcgucg uggggagcgg ccugcuggac gagacccaga ucgagcucgu ggaccggauc      960

cacgccgucu acgacgccgu ggcgcugaag gggauccugg gcgacgccca cacgaucucc     1020

gaggccaagg uggagagcua cgagaagcac cgcaagaacu ggaagcucau caaggaggag     1080

cugcggacca aggagaacaa ggacgagguc gaccuguggu acggggaccu cuccggccgc     1140

caguacaaga acccgcccga ccccgagaag ggccccgacg aggggagcaa gaaggacucc     1200

cccauccggg acagcgacca gaagcagaac cugaucaaga aggccaacga cuacuucacc     1260

gagcugaucg acgacuccca gcucagcgag gagcagaaga acugccugaa gaacgacauc     1320

aacaacggcc agcuguuccc ggugcagcgc uccagcgaca acggggugau ccccuaccag     1380

auccaccuca acgagcugcg gcgcaucauc gagaaccaga agcaguacua ccccuuccug     1440

gccgagaccu ucauccggaa gacggcggag ggcaaguccu acgaggagaa caagaucguc     1500

gcccuccuga aguuccgcgu gcccuacuac guggggccgc uggucagcaa ggaggacaug     1560

gaggagcacu ccagcggcaa cuccgccuac cacuggaugg cccggaaccc cggccgcggg     1620

gaggccauca cccccuggaa cuucgacgag guggucgaca aggacgagag cggcgcgcgg     1680

uucaucuccc gccucaccgg gaccgacacg uaccugcugg gcgagcccac ccucccgcag     1740

cacagccugc uguacgagga guacauggug cuguccgagc ucaacaacgu gcggcugaac     1800

guccgccagg ggaaccacug gagcgacaag aaccggcagc gccugagcua caagcagaag     1860

gaguuccuca ucgagaaccu guucaaggag cacaagaccg ugacccgcaa gcgggccgag     1920

gacugccugu ccagcauggg cugcggcgag gugcagcugu ucgggcuguc cgacgagaag     1980

aaguucgcca gcucccugag cuccuacauc acgcucaaga agauccuggg caaggccuuc     2040

gucgacgaca gcgggaacuc cgcgauccug gagcgcauca ucgaggugca gaccguguuc     2100

gaggaccggg agccccucac ccaccagcug agccugaucg acucccucac cgucgagcag     2160

cgcaagcagc uggccaagau ccacuacacg ggcuggggca agcugagccg gaagcuccug     2220

accuccaaga aggggagcua caagauccac ucccagaacg agaucaugcg cacccagcac     2280

agcaucaucg acaucaugcg gggcgacucc cugaaccuca ucgagaucau ccgcgacaag     2340

aagaucggga ucggcgagug gaucgacaac gagaaccugg accgggagac cggggagagc     2400

gacgugagcc gccuggaguc cguguucgag ggcaucguca ugagcccccg ggugaagcgc     2460

ggcguggucc aggccgugcg ggugaucgac gaccucgcca aggccaucgg gaacccgccc     2520

cagcgcaucu ucaucgagau ggcggacgag guccaggccu cccagcggac ggugagccgc     2580

cugucccggc ugaaggcccu cuacggcagc cccucccugc agaaggaguu caacgaccug     2640

aaggaccucc gcaagagccu gaacgagacc aaggacgccg accugcagga ggaccggcuc     2700

uaccuguacu acauccagcu ggggaaggac cucuacaccg gcaaggagau cuccaucgac     2760

agccugucca gcgccuacga caucgaccac aucgugcccc aggcgcugac ccaggacgac     2820

uccaucgaca accgcguccu cgugagccgg aaggagaacg cccgcaagac ggacuccuac     2880

cuguacaccc ccgaccugau caagaacaug cgcccgcucu gggggaagcu gcuggccgcc     2940

ggccucauga gccagaagaa guacgacgcc cugacccggg uggacgacuu caaggaccgc     3000

gagaagcagc gguucgucca gcgcucccug guggagaccc ggcagaucau gaagaacgug     3060

agcacgaucc uccgcaccua cuacggcgag gacaccaacg ucgugacccu gaaguccagc     3120

aucacccacg acaugcggaa guaccugggg uuccccuaca agaaccgcga caucaacgac     3180

uaccaccacg cgcaggacgc ccucugcauc agcgccgccg gccaguucau gcugaaccgg     3240

cacuucgugu ccgggggcga ggucacggac ggggcgagca acgccuacaa cauguaccug     3300

gccgaguacc uccaggaguu ccgcgacgcc aaggcccggg gcgagcgcaa gcgguccacc     3360

gcguucggcu ucgugguggg gagcaugucc agcaaggacg acgagaagcg caccgaccac     3420

cggaccggcg agaucgucug guccgagacg aacgccgagu accugcgcaa ggugaugaac     3480

uacaagaaga ugcugaucac ccagaaggug ggggacguca agacccgggc ccucuacgac     3540

gagacccgcu acggccacaa ggagagcucc gccaagagca ucaaguucga caaccugcgg     3600

cgcgacacgu cccuguacgg gggcuucagc ggcgccucca ccgcguacau gaugcucgug     3660

gaguacaaga agaagaacaa gcuggugagc aucaccgccc aggaggccuc ccugcucgcc     3720

gucgccgacc agaagaacga caccgugagc gugauggacg ggauccuggc gucccacggc     3780

cugagcgggg ccaagauccu ccugcccaag aucgucacgg gccagcugcu cgacuacucc     3840

gacgcccugg ugacccugca gagcgccagc gagcucaaca ccgcgcgcca gauguggcug     3900

ccgcggaacg acuacaacga caucgacauc auccugaagu ccaaggacau cgaggacgcc     3960

gcccacaagc ucggcuacga cagcgacacc gugcacgucc ugcgccggau ccagaagcug     4020

uucgaggccg uguuccgcgu ggccgagucc caguuccccc uccaccagcu gagcaaggac     4080

cagcaggagg agauccugcg ggacccccag aacuacgggu ucggcuccgc ggucaagcuc     4140

cucgggggcc ugcucaaggc ccugcacgcc aacgccgaga acacgaaccu ggccgggcuc     4200

gugaaggugc ugcacggcga caaggaguac agcgacgacg gcaaggcgaa ggaguucaag     4260

aaguucgggu ucagcccccg cuggcggcgc aucuccaacc cgaccggcuu cacccugucc     4320

gacagcgaca ccuucaucua ccaguccccc agcgggaucu ucgagcagcg ggucacggug     4380

aaggagcuca aggagaaggc cgacaagcag aaguga                               4416


<210>  2233
<211>  4422
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2233
augaagaaca uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucgag       60

aaggacaccg accagaagaa cgggaacauc cucggcacgg ggucccgcau cauccccaug      120

agccaggaca ucaaggacga guucggcaag ggcaacucca ucagccagac cgccgaccgg      180

accaaguucc gcuccgugcg gcgccugcgg gagcgccacc uccuccggcg cgagcggcug      240

caccgcgucc ugaacgugcu cggguuccug ccgaagcacu acgcggacca gaucgacuuc      300

aagaagcggc ugggcaagcu cgugaacgag accgagccca agcuggucua cgacaacaag      360

aacuucaucu ucaagaaguc cuucgaggcc augcugagcg acuucaacca gcaccagccc      420

cuccugcugg ccgacgggaa gaaggugccc uucgacugga cgaucuacua ccuccgcaag      480

aaggcccuga ccgagaagau cgagaaggag gagcuggccu ggcucauccu gaacuucaac      540

cagaagcggg gcuacuacca gcugcgcggg gaggaggagg aggagaaccc gaacaagaug      600

guggcguucc acagccucaa gaucgucgag gugaucgccg acgaguccca gaagggcaag      660

gccgagaccu gguacagccu gauccuggag aacggcugga ucuaccggcg cuccagcaag      720

acgccccucu acgacuggaa ggggaagcag cgggacuuca ucgugaccac cgaccugaac      780

gacgacggcu ccaucaagac cgacaaggag gggcuggaga agcgcagcuu ccgggccccc      840

gucgaggacg acuggacccu cgugaagaag aagacggagc aggagaucaa ccgcucccac      900

aagaccgugg gcaccuacau cuacgagaac cugcugcagg accccaagca gaagaucaag      960

ggcaagcucg uccggaccau cgagcgcgag uucuacaagg acgagcugaa gaugauccug     1020

aagaaccagc agaaguucca cccggagcuc aacgacgagc accuguacau caacugcgug     1080

cgcgagcugu accggaacaa ccaggcgcac cagacgaucc ucagcaagaa ggacuucgug     1140

caccuguuca ucgacgacau caucuucuac cagcgccccc ugcgguccca gaaguccagc     1200

aucagcaacu gcacccucga guuccgcaag uacaagaacg agaacgggac cgaccagacc     1260

gacuaccuga aggccauccc caagagccac ccccuguucc aggaguuccg gcucuggcag     1320

uggaucuaca accugggcau cuaccagaag gaggacgaca cgaacgucac cgacgagcug     1380

aucaagaaca ucgacgggua ccaggaccuc uucgacuucc ugaacaacaa gaaggagauc     1440

cgccaggaca uccugaucaa guuccuggug gagcagaccg gccucaaggg gaagccgcug     1500

uccagcgugg ucgccaagua ccgguggaac uucguggagg acaagaucua ccccugcaac     1560

gagaccggca cgcagauccg cacccggcug gccaaggugg agaacacccc cgaggacuuc     1620

cucaccgccg agacggagau caagcugugg cacaucaucu acuccgucaa cgacaaggcg     1680

gacuacgaga aggcccugaa gaccuucgcc gagaagcaca accucgacac ccacagcuuc     1740

guggagaacu ucaagaaguu cccgcccuuc aaguccgagu acggcagcuu cuccgagaag     1800

gccaucaaga agcugcugcc ccucauccgc cuggggaagu acuggagcug ggagaacauc     1860

gacaagaaga cccaggagcg gaucgagaag auccugacgg gcgaguucga cgaggccauc     1920

aagucccgcg ugcgggacaa ggcgauccac cucaccgagg aggaccacuu ccaggggcug     1980

cagcuguggc ucgccagcua cgucguguac ggccgccacu ccgaggccga caaccuggac     2040

aaguggcaca ccgucgccga ccuggaggac uaccucgccg aguuccggca gcacagccug     2100

cgcaacccca ucguggagca ggucaucacc gagacgcugc ggguggugaa ggacaucugg     2160

cuccaguacg ggcagggcgc gaaggacuuc uucuccgaga uccacaucga gcuggcccgc     2220

gagaugaaga acaccggcga cgagcggaag cgccugacca gccucgugac cgacaacgag     2280

aacaccaacc ugcgcaucaa gguccugcuc uccgagcugc ugaacgacgg gaacguggag     2340

aacgugcggc ccuacagccc gaugcagcag gaggcccuca agaucuucga ggacggcguc     2400

cugaacuccg acaucgagau cgaggacgac auccugaaga ucagcaagau ggcccagccc     2460

acgaacaccg agcucaagcg cuacaagcug uggcuggagc agaaguaccg gucccccuac     2520

accgggcaga ucaucccccu caaccgccug uucaccgccg acuacgagau cgagcacauc     2580

aucccgcaga gccgguucuu cgacgacagc uucuccaaca agaucaucug cgagagcgcg     2640

gugaaccagc ugaaggacaa gcagcucggc cuggaguuca ucaagaacca cuccgggacg     2700

aucgugaagc ugggcauggg caagaccgcc aagauccucg acgucgagag cuacaacgag     2760

uucgugaagg ggaacuacac caagaacauc uccaagcgca acaagcugau gcuggaggag     2820

auccccgaca agaugaucga gcggcagcuc aacgacaccc gcuacaucag caaguucauc     2880

aucuccgccc ugagcaacau cgugcggucc gagagcaacg acgacggcuu caacuccaag     2940

aacguccugc ccgggaacgg caagaucacg agccgccuca agcaggacug gggcaugaac     3000

gacaucugga acgagcugau ccugccccgg uucgagcgca ugaacaagau caccgacucc     3060

gccguguuca ccgcguggaa cgagaacaac cagaaguucc ucccgaucgu gccccuggag     3120

uucagcaagg gguuccagaa gaagcggauc gaccaccgcc accacgcccu ggacgcccuc     3180

gucaucgccg ccuccacccg ggagcacauc aaccugcuga acaacgagca cgccaagagc     3240

gacaagcgcu ucgaccucaa ccggaagcug cgcaaguucg agaagguggc guacaacgac     3300

cccaagacgg gcaagcggau cgagaaggag gugccgacca ucuuccugaa gcccugggag     3360

accuucaccg ucgacgccaa gaacucccuc gagagcauca ucgugagcuu caagcagaac     3420

cugcgcguga ucaaccgggc cacgaacuac uccgagagcu acaacgacga gaacggggcc     3480

cugcgccucg gcaaggacgg gaacccccug aaggagaaga ucaagcaguc caagggcgac     3540

agcugggcga uccgcaagcc caugcacaag gacuucgucu acggcaaggu gcaccuccag     3600

aacgugaagc ugccgaagga caaguucauc accgccaucc ggaagucccu ggacgucacc     3660

uucgaccuga agcgcaucga gaccaucacg gacaccggga uccagaagau ccucaagaac     3720

uaccugcaca gcaaggagaa caagguggag cuggccuucu cccccgaggg caucgaggac     3780

augaaccagc acaucgagcg guacaacgac gggaagaagc accagcccau ccacaaggug     3840

cgcgucuucg agaucggcag ccgguucucc cucggggaga gcggcaacaa gaagaccaag     3900

uucguggagg ccgccaaggg caccaaccug uucuucgcgg uguaccagga cgacaacaag     3960

gggaagcgca acuacgagac gaucccccug aacaucguca ucgagcggca gaagcucggc     4020

cugcacuccg ugccgaccga ggacgccaag ggcgggaagc ugcuguucca ccugagcccc     4080

aacgacaugg uguacguccc caccuccgag gagcuggagg acaaccucgc cagcgugaac     4140

aucaagcgcc ugaacaccgc ccagcggaag cgcaucuaca agguggagaa ggcguccggg     4200

gucgagugcu acuucauccg gcaggacauc gccuaccuga ucaagaacua cgacgccaag     4260

acgaagaucg gcgagcucga gagccagaac aagcugcaga ccaccaugac cgacgaccgc     4320

cugaagauca cggacgugug cgugaagcuc aacaucgacc ggcugggcuc caucgccgag     4380

accuuccugg ggaaccagua cggcgccagc aagaagaccu ga                        4422


<210>  2234
<211>  4422
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2234
auggccaaga uccugggccu cgaccugggg accaacagca ucggcugggc cguggucgag       60

cgcgagaaca ucgacuucuc ccugaucgac aagggggugc ggaucuucag cgagggcgug      120

aaguccgaga aggggaucga gagcucccgc gcggccgagc ggaccggcua ccgcagcgcc      180

cggaagauca aguaccgccg gaagcuccgc aaguacgaga cgcugaaggu ccugucccuc      240

aaccggaugu gcccccugag caucgaggag guggaggagu ggaagaaguc cggcuucaag      300

gacuacccgc ugaaccccga guuccucaag uggcugagca ccgacgagga guccaacgug      360

aaccccuacu ucuuccgcga ccgggccagc aagcacaagg ucucccuguu cgagcucggg      420

cgcgccuucu accacaucgc gcagcggcgc ggcuuccuga gcaaccggcu ggaccagucc      480

gccgagggga uccucgagga gcacugcccc aagaucgagg ccaucgugga ggaccugauc      540

agcaucgacg agaucuccac caacaucacc gacuacuucu ucgagacggg cauccuggac      600

agcaacgaga agaacgggua cgccaaggac cucgacgagg gcgacaagaa gcuggugucc      660

cuguacaaga gccuccuggc gauccugaag aagaacgaga gcgacuucga gaacugcaag      720

uccgagauca ucgagcgccu caacaagaag gacguccugg gcaaggugaa ggggaagauc      780

aaggacauca gccaggccau gcuggacggc aacuacaaga cccucgggca guacuucuac      840

ucccuguaca gcaaggagaa gauccggaac caguacaccu cccgcgagga gcacuaccug      900

agcgaguuca ucaccaucug caaggugcag ggcaucgacc agaucaacga ggaggagaag      960

aucaacgaga agaaguucga cggccucgcc aaggaccugu acaaggccau cuucuuccag     1020

cggccgcuga agucccagaa ggggcucauc ggcaagugca gcuucgagaa guccaagagc     1080

cgcugcgcca ucucccaccc cgacuucgag gaguaccgga uguggacgua ccugaacacc     1140

aucaagaucg ggacccagag cgacaagaag cugcgcuucc ucacccagga cgagaagcug     1200

aagcuggucc ccaaguucua ccgcaagaac gacuucaacu ucgacgugcu cgcgaaggag     1260

cugaucgaga agggcuccag cuucggguuc uacaagucca gcaagaagaa cgacuucuuc     1320

uacugguuca acuacaagcc cacggacacc guggccgccu gccaggucgc cgccucccug     1380

aagaacgcga ucggcgagga cuggaagacc aagagcuuca aguaccagac caucaacucc     1440

aacaaggagc aggucagccg gacgguggac uacaaggacc ucuggcaccu ccugaccgug     1500

gccaccagcg acguguaccu guacgaguuc gccaucgaca agcugggccu ggacgagaag     1560

aacgccaagg ccuucuccaa gaccaagcuc aagaaggacu ucgcgagccu gucccugagc     1620

gccaucaaca agauccugcc guaccucaag gaggggcugc uguacuccca cgccguguuc     1680

gucgccaaca ucgagaacau cguggacgag aacaucugga aggacgagaa gcagcgcgac     1740

uacaucaaga cgcagaucag cgagaucauc gagaacuaca cccucgagaa gucccgguuc     1800

gagaucauca acggccugcu gaaggaguac aagagcgaga acgaggacgg gaagcgcgug     1860

uacuacucca aggaggccga gcagagcuuc gagaacgacc ucaagaagaa gcugguccug     1920

uucuacaagu ccaacgagau cgagaacaag gagcagcagg agaccaucuu caacgagcuc     1980

cugcccaucu ucauccagca gcugaaggac uacgaguuca ucaagaucca gcggcucgac     2040

cagaaggugc ugaucuuccu gaagggcaag aacgagaccg ggcagaucuu cugcacggag     2100

gagaagggca ccgcggagga gaaggagaag aagaucaaga accgccucaa gaagcuguac     2160

caccccagcg acaucgagaa guucaagaag aagaucauca aggacgaguu cggcaacgag     2220

aagaucgucc uggggagccc ccucaccccg uccaucaaga accccauggc caugcgggcc     2280

cugcaccagc ugcgcaaggu gcucaacgcc cugauccugg agggccagau cgacgagaag     2340

accaucaucc acaucgagau ggcgcgggag cucaacgacg ccaacaagcg caaggggauc     2400

caggacuacc agaacgacaa caagaaguuc cgggaggacg ccaucaagga gaucaagaag     2460

cuguacuucg aggacugcaa gaaggaggug gagcccacgg aggacgacau ccugcgcuac     2520

cagcucugga uggagcagaa ccgguccgag aucuacgagg agggcaagaa caucagcauc     2580

ugcgacauca ucggguccaa ccccgccuac gacaucgagc acaccauccc gcgcagccgg     2640

agccaggaca acucccagau gaacaagacc cugugcagcc agcgcuucaa ccgggagguc     2700

aagaagcagu ccaugcccau cgagcugaac aaccaccucg agauccugcc ccgcaucgcc     2760

cacuggaagg aggaggcgga caaccugacc cgggagaucg agaucaucag ccgcuccauc     2820

aaggccgccg ccacgaagga gaucaaggac aagaagaucc gccggcgcca cuaccucacc     2880

cugaagcggg acuaccugca gggcaaguac gaccgcuuca ucugggagga gcccaaggug     2940

ggcuucaaga acagccagau cccggacacc gggaucauca ccaaguacgc ccaggcguac     3000

cucaaguccu acuucaagaa gguggagagc gucaagggcg ggaugguggc cgaguuccgg     3060

aagaucuggg gcauccagga guccuucauc gacgagaacg gcaugaagca cuacaaggug     3120

aaggaccgca gcaagcacac gcaccacacc aucgacgcca ucaccaucgc cugcaugacc     3180

aaggagaagu acgacguccu ggcccacgcg uggacccugg aggaccagca gaacaagaag     3240

gaggcccggu ccaucaucga ggccagcaag cccuggaaga cguucaagga ggaccuccug     3300

aagaucgagg aggagauccu ggugucccac uacacccccg acaacgugaa gaagcaggcc     3360

aagaagaucg uccgcgugcg ggggaagaag caguucgugg cggaggucga gcgcgacgug     3420

aacggcaagg ccgugcccaa gaaggccgcc agcgggaaga ccaucuacaa gcucgacggc     3480

gaggggaaga agcugccgcg gcugcagcag ggcgacacca uccgcggcuc ccuccaccag     3540

gacagcaucu acggggccau caagaacccc cugaacacgg acgagaucaa guacgucauc     3600

cggaaggacc uggaguccau caagggcagc gacguggagu ccaucgugga cgaggucgug     3660

aaggagaaga ucaaggaggc gaucgccaac aaggugcucc ugcugagcag caacgcccag     3720

cagaagaaca agcucgucgg gaccgugugg augaacgagg agaagcgcau cgccaucaac     3780

aaggugcgga ucuacgccaa cuccgucaag aacccccugc acaucaagga gcacagccug     3840

cucuccaaga gcaagcacgu gcacaagcag aagguguacg gccagaacga cgagaacuac     3900

gcgauggcca ucuacgagcu ggacgggaag cgcgacuucg agcugaucaa caucuucaac     3960

cucgccaagc ugaucaagca gggccagggc uucuaccccc ugcacaagaa gaaggagauc     4020

aaggggaaga ucgucuucgu gccgaucgag aagcggaaca agcgcgacgu gguccucaag     4080

cggggccagc aggucguguu cuacgacaag gagguggaga accccaagga caucuccgag     4140

aucguggacu ucaaggggcg caucuacauc aucgagggcc ugagcaucca gcggaucgug     4200

cgccccuccg ggaaggucga cgaguacggc gugaucaugc ugcgcuacuu caaggaggcc     4260

cggaaggccg acgacaucaa gcaggacaac uucaagccgg acggcguguu caagcucggg     4320

gagaacaagc ccacccgcaa gaugaaccac aaccaguuca ccgcguucgu cgagggcauc     4380

gacuucaagg ugcugcccag cgggaaguuc gagaagaucu ga                        4422


<210>  2235
<211>  4425
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2235
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucgag       60

uccgacuucg agaagaagca ggggaagauc aacggccucg ggagccgcau cauccccaug      120

ucccaggaca uccugggcaa guucgacagc ggcgugucca ucagccagac cgccgagcgg      180

acguccuacc gcgggguccg gcgccugaac cagcggaacc uccugcgccg ggagcgccug      240

caccgggugc ucaacauccu ggacuuccug ccgaagcacu acagcaacgc gaucgacuuc      300

gagaagaagc ucggccaguu caaggagaac guggaggaga agcugaacua cgccaagaac      360

cagaucgggc gccacgaguu caucuucaug gacuccuuca aggagauggu caaggaguuc      420

aagcagaccc agccccagcu guucuacaag cgggagaacg gcaucgagac caagaucccc      480

uacgacugga cccucuacua ccugcgcaag aaggcccuga gccagaagau caagaaggag      540

gagcucgccu ggaucauccu gaacuucaac cagaagcggg gguacuacca gcugcgcggc      600

gaggacgugc ucgacgaguc caagaucgag gacuucuaca gccugaaggu ggucgaggug      660

caggagacgg aggacaagaa cgcccggggc accugguaca acgugauccu ggagaacgac      720

ugggucuaca agcgccaguc caaggagagc cucgacaacu ggaucgggaa gaacaaggag      780

uucaucguga ccaccaacaa ggagaaggac ggcucccuga agcuggacaa ggcggggaac      840

gugaagcgga acuuccgcgc cgucgacagc gagaaggacu ggaucgccau caagaagaag      900

acggagcagg acguggagau cuccaagcac accgugggcc aguacaucua cgagacccuc      960

cugcagaacg ccaagcagaa gaucaagggc aagcugguca agaccaucga gcggaaguuc     1020

uacaaggagg aguucaucaa gaucaucaag gagcagcuca agcaccaccc cgagcugcag     1080

agcaagacgc uguacgagac cugcauccag gagcucuacc cgcgcaacga ggcgcaccag     1140

aacaacauca aguccaaggg guucgaguac cuguucaccg acgacaucau cuucuaccag     1200

cggccccuca agagcaagaa guccaccauc agcggcugcc aguacgagac gcgccucuuc     1260

aagaagaccg ugcugaacaa ggugaccaac aaggagaacg agaucuggca gaaggagccc     1320

cugaaggcca ucagcaaguc ccacccccuc uuccaggagu uccgccugug gcaguuccug     1380

aagaaccuca agaucuacca gaaggaggac gucaagggga accugaucga ccacgacauc     1440

accagcgacc ugcucaagac ggaggccgac uggguggagc uguucgacuu ccugaacauc     1500

aagaaggaga ucgagcagaa gcagaucauc caguucuuca ucaacaacaa gcagaucgag     1560

aagggcaaga agaacaacua ccgguggaac uacguggagg acaagaagua cccgugcaac     1620

gagaccaagu cccagcucau cagccgccug aagaagguca agaaccugga ccugaaccuc     1680

uucuucaccu ccgagaccga gcugaagcug uggcacauca ucuacagcgu gaaggacaag     1740

auccaguacg agaaggccau caagaacuuc gccaucaaga acaacaucga caucgacucc     1800

uucguggaga acuucaagcg guucccgccc uucaagaacg aguacgcggc cuacagcgag     1860

aaggccauca agcgccuccu gccccugaug cggauggggg aguacuggaa cgaguccgag     1920

aucaagcagg aggucaagga ccgcgccuac agcaucaucg agcggcucaa gggcaucgac     1980

uucaacaagg acaagaucga cgagaagauc acggacgacg acaucaagaa gcagcugcug     2040

aaguccuuca ucgacuucaa ggacaagaac ccccucaagg gccugaacac cuaccaggcc     2100

ugcuaccugg uguacaaccg ccacagcgag gucaacucca ucgcguacug gaaguccccc     2160

gacgacauca acaaguaccu cgagggguuc aagcagcaca gccugcggaa ccccaucgug     2220

gagcaggucg ugaccgagac ccugcgcacg gugcgggaca ucuggcagua cuacggcaag     2280

gggaaggcca gcuucuucaa cgagauccac guggagcucg gccgcgagau gaagaacccc     2340

gccuacaagc ggaaggccuc caccaaccgc gucaccgaga acgagaacac caaccaccgg     2400

aucaaggaga uccugaagga gcugcagaac gagggggugc aggaggucaa gagcuacagc     2460

cgcaaccagc aggagauccu caagaucuac gaggagggca ucuaccaguc caucgacgug     2520

guggacgacg ccaucgagaa gauccggaag aacagcuccc cgacgaaggc ggagaucauc     2580

aaguacaagc uguggcugga gcagggcuac accagccccu acaccgggaa gaccaucccc     2640

auguccaagc uguucacgac cgacuaccag aucgagcaca ucauccccca gagccuguac     2700

uucgacaacu cccugagcaa caagaucauc ugcgaguccg aggugaacca gcucaaggac     2760

aaccagaccg ccuacgaguu caucaagaag cacggcaccg agaaggucag ccuggggaag     2820

aacaagaccg uggagaucuu cacgcuggag caguacgaga agcacugcaa ccaguacuuc     2880

aagaagaaca agaacaagcu caagaagcug cuguccgagg agaucccgga gggcuucauc     2940

aaccgccagc ucaacgacag ccgguacauc uccaaguuca ucaaggggcu gcugggcaac     3000

cucgugcgcg aggaggacga gcaggagguc accaccaagc acaucgugcc cgugaccggc     3060

gccaucacga gcaagcugaa gcaggacugg gggcugaacg acaaguggaa cgagcucguc     3120

gccucccgcu uccagcggcu gaacaccaug accaacagca acaacuucgg cuacugggac     3180

accaagauca acgccuuccg cguccaggug cccgacgaga ucuccaaggg guucagcaag     3240

aagcggaucg accaccgcca ccacgcgcug gacgcccucg ugaucgccug cugcacgcgg     3300

aaccacauca acuaccugaa cgcccugaac gcggagaaga agaacuacgg ccuccgcgac     3360

cugcugcuca ucaagaacga caagaaccac uacaccaagu ucuucaagca ccccugggac     3420

ggcuucccgg uggaggccaa gagcgugcug gagaagacca ucaucuccuu caagcagaac     3480

acccggguca ucaacaagac gaacaacaag accuggcagu gggccgagaa ggaggggcag     3540

cugaagaagg ugcucgugaa gcagaccaag ggcgacaacu gggccauccg caagcccaug     3600

cacaaggaga ccgucagcgg gaagcugaac auccccacgc ccaagggcaa gaucgccacc     3660

gcgucccggg ugagccuguc cgagaucaag acccgcaagc agcucgacaa gaucaccgac     3720

aucagcaucc agaagauccu ggagcggcac cugaacaacu accucgacga caaggggaag     3780

gagaaguucg accuggccuu caacgccgac ggcguggagg agcugaacaa gaacaucaag     3840

guccucaaca agggcaagaa ccaccagccg aucuacaagg ugcgccugua cgagaucggg     3900

ucccgguuca aggugggcaa cagcgggaac aagaaggaca aguacgucga ggccgccaag     3960

ggcacgaacc uguucuucgc gaucuaccuc aacgagaagg acaagaagcg cguguacgag     4020

accauccccc ugaacgaggu gaucgagcac cagaagcagg ucuccaaccu gcccaagaac     4080

gagcggaccc cggugcccgu gaaccacacc aaggggaagu uccucuucag ccuguccccc     4140

aacgaccugg ucuacgugcc cacggacgag gagaucgaca acccgagccu cgugaacuuc     4200

aacaaccuga acaagaagca ggucgagaag aucuacaaga ccguguccug cagcaccuac     4260

cagugcuucu ucgugcacaa caccaucucc aagcccaucc agaacaaggu cgaguucagc     4320

gcccugaaca agauggagaa guccaucgac ggcaucauga ucaaggaccg cugcuggaag     4380

cucaagaugg accggcuggg caacaucauc agcgugaucc gcuga                     4425


<210>  2236
<211>  4443
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2236
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc caaggugugc       60

gucgacaacg acgggaacua ccugcgcgac aucaagcucg gcucccggau cauccccaug      120

agccaggaca cgcuggggaa cuucgacaag ggcgugaccg acuccccgac cgccgcgcgc      180

accggcuucc gcggggugcg gcggauccgc gagcgggccc ugcagcgccg ggagcgccuc      240

caccgggucc ugcacgugau gaaguuccug ccgccccacu acgagaacgc caucggcugg      300

aaccgcgagg aggccacgac cuacgggaag uuccucaacc acggcgagcc caagcuggcc      360

ugggagaagc aggaggacgg gagcaugcgg uuccuguuca uggacuccuu ccacgagaug      420

auggcggacu ucgccgagag ccagcccggc cucguggccg gcgggaagaa gaucccccug      480

gacuggaccc uguacuaccu ccgcaagaag gcccugaccc aggcgauccg gaaggaggag      540

cuggccugga uccuccugaa cuucaaccag aagcgcggcu acuaccagcu gcgcggggag      600

gaggacgagg agaaccccgc caagcgggag gaguaccacg agcucaaggu cguguccgug      660

gaggccgccg aggagggcaa gggcgggaac acgugguaca acgucacccu ggagaacggc      720

uggaucuacc ggcgccagag caagguggag cugcacgacu ggguggggaa gacccggcag      780

uucaucguca ccacggagua cgagaaggac ggcgugacca ucaagaagga caagaacggg      840

gaggugaagc gcuccuuccg cgcgcccagc gaggacgacu ggggccuccg gaagaagcgc      900

accgaguccc ugcuggagga cagcggcaag accgucgggg ccuucaucua cgaccacauc      960

cucuccgagc cgacggacaa gauccggggc aacuucaucc gcaccaucga gcggaaguac     1020

uacaagaagg agcuggaggc cauccugcuc gagcagacca agcaccacaa ggagcugaag     1080

gaccagaaca cccugaucgc cugcaucaag gagcucuacc agagcaacaa gccccaccag     1140

gacucccuga ugaagaagga caugacguac cugcucauca ccgaccugau cuucuaccag     1200

cgcccccuca agagcaagaa gucccucauc gccgacugcc ccuacgagua cuacgaguac     1260

accaucaagg agaccgggga gaucaagcgg cagggcauca agugcaucgc caagagcaac     1320

ccguacuacc aggaguuccg ccuguggcag uucaucauca accugcggcu guucgaccgc     1380

acggacgaca aggaggugac cuccgaguac cugagcagca uggaggacua caccaagcug     1440

uucaccuacc ucaacgaccg gaaggacauc aaccaggaga cgcugcugaa ggacuucuuc     1500

aagcagaaga aggugaagau ggggauggag aaggaguucc ccauccgcug gaacuacauc     1560

gaggacaagg agaagaagua ccccugcaac gagacccggc acgagcuccu ggcggcccug     1620

gacaaggccg gcaucgacca cuccuggcug gacgacaagg agcgcuucua ccggcucugg     1680

caccuccugu acagcgucga guccaaggag gaggccgagg gcgcgcuccg caagcugaag     1740

gacaacgacg acuucgugaa cgccuuccug aaggugaagc ccuucaagaa ggacuacggg     1800

gccuacagcg agaaggccgu caagaagcuc cuggccauca ugcggauggg cgggcugugg     1860

aacgagaccg acaucugcga gcagacccgc aaccggaucc aguccaucau caagggcgag     1920

aucgacgaga agaucaagga gcgcaugggg cgcacguucu gccagcucag cgacuuccag     1980

ggccugccgg aguggcuggc gugcuacgug guguacggcc ggcacuccga ggccaccgac     2040

auccagcgcu ggaccaagcc cgagaaccuc auggccuaca ucaacggguu caagcagcac     2100

agccugcgga accccaucgu cgagcagugc auccuggaga cccuccgcac ggugcacgac     2160

aucuggaagg aggccggcca caucgacgag auccacgucg agcuggggcg guccaugaag     2220

uccaccgcgg accagcgcgc ccggaugagc aagagcaucc ugcagaacga gaacaccaac     2280

cuccgcauca agucccugcu gauggagcuc aagaacgcca aggagaucga gaacgugcgg     2340

cccuacagcc cgaugcagca ggagauccug cgcaucuacg aggagggcgc ccugcaggag     2400

cucaccaagg aggacgccca guaccccgag aucuccaaga ucagccagau ggcgcagccc     2460

uccgccaagg agcugcugaa guaccggcuc uggcuggagc agaaguacug cagccccuac     2520

accggcaagc cgaucucccu gagcaagcug uucacgagcg ccuaccagau cgagcacauc     2580

aucccccagu cccgcuacuu cgacgacagc uucaccaaca aggugaucug cgagaccgag     2640

gucaaccagc ugaaguccaa caugcugggg uacgaguuca ucaagaagca cagcggcgag     2700

aucgugcacu gcaccacgau cggggacgug aagauccucg acgccaagga guacuacaac     2760

uucgucaccg agcacuacgc caacaaccgg cgcaagaagg agaagcugcu gcucgaggac     2820

aucccccagg aguuccugaa ccggcagaug aacgacuccc gcuacaucag caagaaggug     2880

auggcgaucc uguccaacau cgugcgggag gaggacgagc aggagaccaa cagcaaguuc     2940

gucguguccu gcagcggcgg gaucaccgac cgccucaagc aggacugggg ccugaacgac     3000

guguggaacu ccaucgucua cccccgguuc gagcgccuga acaagcucac gcagaccgag     3060

gccuucggcc ggugggagaa caaggagggg aagcaggucu uccagaccag ccugccgcug     3120

gagcuccagc gcggcuucuc caagaagcgc aucgaccacc ggcaccacgc cauggacgcc     3180

cuggugaucg ccugcgcgag ccgcaacauc aucaacuacc ugaacaacga guccgccaag     3240

agccaggagc agcgggagga ccuccgcaag aagcugugcg acaagaaccg gaucauccgc     3300

aagcccuggg agaccuucac gcaggacgcc cggaccgccc uggaggacgu gguggugucc     3360

uucaagaacu acguccgcgu gaucaacaag gcgaccaacu acuacgagaa guacgacgag     3420

agcgggaaga agggcauggc cgagcagaag gggcaggaca ugugggccau ccggaagccc     3480

augcacaagg agaccguguu cggccagguc aaccuccgcc ggaaggugac ggugaagcug     3540

aaggacgccc uggagaacau cccggccauc ugcgacaagg cgcuccgcga cuacgucaac     3600

gaccugaucg ccaagaaguu caacaccaag cagaugcugg cccacuucaa gagcaucaac     3660

uaccggcuca acaugaaguc cgugaacaag gugggcaucu gggucuucag cgacgagaag     3720

gagcccaugg uggccacccg caaggccauc gacaucuccu ucgacgcgaa gcggaucgac     3780

accaucacgg acaccgggau ccagcagauc cugugcaagu accucaagag caagggcaac     3840

gaccccaacg uggccuucac ccccgagggg aucgccgaga ugaacaagca caucaucgac     3900

uacaacaacg gcaagcagca ccagccgauc cugaaggucc gcguguccga gcccaagggg     3960

gccaaguacc aggugggcga gaccggcaac aagacgaaga aguucgucga ggcccagaag     4020

gggaccaacc uguacuucgc gaucuacgag gacaaggacg gcaaccggag cuacaacacc     4080

aucccccuga acgagguggc cgagcgccuc aagcaggggc ugucccccgu gccggagaag     4140

aacgagaagg gcaucagccu gaaguucuac cucuccccca acgaccuggu cuacgugccc     4200

accgaggagg agaagcugag caacgagugc aagcucucca aggagcggau cuacaagaug     4260

gugagcucca cgagcaacga gugccacuuc auccccuaca ccaccgccaa gccgaucuac     4320

gacaaguucg aguacgaggc caagaacaag auggggcgcg cgcugaccgg cgagaugauc     4380

aaggccgucu gcuggaagcu ggagguggac cgccucggca acauccugaa gaucaucaag     4440

uga                                                                   4443


<210>  2237
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2237
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc caagaucucc       60

aucgacgaca acgggaacua ccugcgcgac auccagaugg gcagccggau cauccccaug      120

ucccaggacg ugcucgggaa cuucgacagc ggcgucacgg agucccagac cgccgugcgc      180

accggcuucc gggggcugcg ccggauccgc gagcggagcc ugcuccgccg ggagcgccug      240

caccgggugc ugcacgucau gggcuuccuc ccgccccacu acgacuccgc gaucgggugg      300

aacaaggagg agcccaccac guacggcaag uuccugaacc acggggagcc caagcuggcc      360

ugggagcgcc agggcgacgg cagcaugcgg uuccucuuca uggacuccuu ccacaagaug      420

auggaggacu ucgccccgca ccagcccgcc cugguggccg acgggcgcaa gaucccccug      480

gacuggaccc ucuacuaccu gcggaagaag gcgcugaccc agcccaucaa gaaggaggag      540

cucgccugga uccugcugaa cuucaaccag aagcgcggcu acuaccagcu ccgcggggag      600

gaggaggagg agaacccgac caagcgggag gaguaccacg agcugaaggu ggucagcgug      660

gaggccgccg aggagggcaa gggcgggaac acgugguaca acgugaagcu ggagaacggc      720

uggaucuacc ggcgcuccag caagaucccc cucgacgacu gggucgggaa gacccggccc      780

uucaucguga ccaccgagua cgaggcggac ggcaucacgc ugaagaagaa caaggagggg      840

gaggugaagc gcuccuuccg cgcccccggc gaggacgacu ggggccugcg gaagaagcgc      900

accgagagcc agcucgaggc cuccgggaag accgucggcg ccuucaucua cgaccaccuc      960

cuggccgagc cggcggacaa gauccggggg aacuucaucc gcaccaucga gcggaaguac     1020

uacaagcagg agcuggccga cauccugcgc gagcagagca aguaccacga ggagcugcag     1080

uccaagaaga uccucgccga cugcgccgag gagcuguacc ggagcaacca gccccaccgc     1140

gacucccugc ucaagaagga caugguguac cugcugcuca cggacaucau cuucuaccag     1200

cggccccuca agagcaagaa gucccugauc gccgacugcc ccuacgagua cuacgaguac     1260

guggacaagg agaccggcga gaucaagcgc caggggauca agugcgucgc caagagcaac     1320

ccguacuacc aggaguuccg gcuguggcag uucgugcuga accugcgccu cuacgaccgg     1380

accgaggaca aggaggugac cgcggaguac cugcccagca aggaggacua cgcccgccug     1440

uucgucuacc ucaacgaccg gaaggagauc aaccaggaga cgcugcugaa ggacuucuuc     1500

aagcugaaga aggugaagca gcgcuccgag aaggaguucc ccgugcggug gaacuacauc     1560

gaggacaagg agaagaagua ccccugcaac gagacccgcc acgagcuccu gauggcccug     1620

gaccgggccg gcaucgagcg cagcuggcuc gacgacaccg cgcgccagug ccggcugugg     1680

caccuccugu acuccaucga ggucaaggac gaggccgagc gcgcccugcg gaagcugaag     1740

gacgacgacg aguucgugag cgccuuccuc aaggugaagc cguucaagaa ggacuacggc     1800

gccuacuccg agaaggcggu caagaagcug cuggccguga ugcgcauggg gagccacugg     1860

ugcgaggacg acaucugcaa ggagaccaag gagcggaucg agcacauccu ccagggcgac     1920

gucgaggaga agaucaagga gcgcaugaac cacacggccu acucccuggg ggagcugagc     1980

gacuuccagg cccuccccga guggcuggcg ugcuacgugg uguacggccg gcacuccgag     2040

gccaccgaca ucaagcacug gaagaccccc gagagccuga uggccuacau caacggguuc     2100

aagcagcacu cccuccgcaa ccccaucguc gagcagugca uccuggagac ccugcggacg     2160

gugcacgaca ucuggaagga ggccggccac aucgacgaga uccacgugga gcucggccgc     2220

agcaugaagu ccaccagcga ccagcgggcc cgcaugaccg agaacauccu gcggaacgag     2280

aacaccaacc ugcgcaucaa gucccuccug auggagcuga agaacgacgc ggacaucaag     2340

gacguccggc cguacagccc caugcagcag gagauccucc gcaucuacga ggagggggcc     2400

cugcaggagc ugacgaagga ggacaacgac uacgaggaga ucuucaagau cuccaagaug     2460

ccccagccca gcgccaagga gcuccugaag uacaagcugu ggcucgagca gaaguaccgg     2520

agcccguaca ccggcaagcc caucucccug agcaagcugu ucaccuccgc cuaccagauc     2580

gagcacauca ucccccagag ccgcuacuuc gacgacuccu ucaacaacaa ggugaucugc     2640

gagagcgagg ugaacgcccu caaggggaac augcugggcu acgaguucau caagaaccac     2700

ggcggggaga ucguccacug cacccugcuc ggcgacguga aggugcagaa cgaguccgag     2760

uacaaggcgu ucgucacgga gcacuacgcc aacaacaagu acaagaagga gaagcugcug     2820

auggaggaca ucccgcagga cuuccucaac cggcaccuga acgacagccg cuacaucucc     2880

aaggugguga agacccugcu cagcaacauc guccggaccg aggacgagau ggagaccacc     2940

uccaaguucg uggugagcug caccgggggc aucacggacc gccugaagaa ggacuggggg     3000

cugaacgacg ucuggaacuc caucguguac agccgcuucg agcggcucaa cgagcugacc     3060

aaguccgacg ccuucggccg cugggagaac aaggagggca agcggguguu ccagaccagc     3120

gucccccugg agcuccagcg cggguucaac aagaagcgga ucgaccaccg ccaccacgcc     3180

auggacgccc uggugaucgc gugcgccucc cggaacauca ucaacuaccu gaacaacgag     3240

agcgccaaca gccccaagaa gcgcgaggac cuccggcagc agcugugcga caagaaccgc     3300

aucauccgga agcccuggga guccuucacg caggacgccc gcgcggcccu ggaggacgug     3360

gucgugagcu ucaagaacua cgugcggguc aucaacaagg ccaccaacua cuacgagcgc     3420

uacgacgccg acggcaagaa gaccaucacc aagcagaagu cccgggagau gugggccgug     3480

cgcaagccga ugcacaagga gacgguguuc gggcacguca accuccggcg caaggcgauc     3540

gugaagcuga aggacgcccu ggagaacauc cccagcaucu gcaacaagga gcuccgggac     3600

uacaucaacg gccuggugga gcgcaacuuc aacaccaagc agcugcacgc ccacuuccgc     3660

uccaccgacu accgguggaa caagcagaac gucgacaagg uggaggugug gcgcuucagc     3720

gacgagaagg agccccucgu cgccacccgg aagccccugg acacguccuu cgacgccaag     3780

cgcaucgaca ccaucaccga caccgggauc cagaagaucc ugcucaacua ccuggaggcg     3840

aagggcggcg acccgcugau cgccuucacg cccgagggga ucgccgagau gaaccagaac     3900

aucgcccucu acaacgacgg caagaugcac cagcccaucc ugaaggugcg gaucagcgag     3960

cccaaggggg ccaaguacca gaucggccag accgggaaca aggcggacaa guucguggag     4020

gcccagaagg gcaccaaccu guacuucgcc aucuacgagg acgaggaccg caaccggacc     4080

uacaacacga ucccgcucaa cgaggucgcc gagcgccuga agcagggccu gccgcccgug     4140

cccgagaaga acgagaagga ggugccccuc aaguucuacc ugucccccaa cgaccugguc     4200

uacgugccca ccgaggagga gauccucaug aaggagugca agcugaacaa gaaccggauc     4260

uacaagaugg ugagcuccac cgggaaccag ugcuucuuca uccccuuccg cgucgcggug     4320

agcaucguga acaagaugga guacuccccg cugaacaaga uggagaaggc caucagcucc     4380

gagaugauca aggccgucug cuggaagcuc gagguggacc ggcugggcaa caucauccgc     4440

guggucaagu ga                                                         4452


<210>  2238
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2238
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc caaggugucc       60

cuggacgaca acgggaacua ccuccacgac aucaagcugg gcugccgcau cauccccaug      120

agccaggacg uccuggacaa cuucgggaag ggcgugacgg ugucccagac cagcguccgg      180

accggcuucc gcggggugcg gcgcauccgg gagcgcuccc uccagcggcg cgagcggcug      240

caccgcgugc ugcaccggcu cggcuuccug ccgcagcacu acgacgccgc gaucgggugg      300

gacagcgacu gcgccaagac cuacggcaag uuccuggaca acggggagcc caagcucgcc      360

ugggagcgcc aggaggacgg cuccaugcgg uuccuguuca ugaacagcuu cuacgagaug      420

aucgccgacu ucacguccca ccagcccgcc cuggucgcgg acggcaagaa gaucccccuc      480

gacuggaccc uguacuaccu gcgcaagaag gcccucaccc aggccauccg gaaggaggag      540

cuggccugga uccugcucag cuucaaccag aagcgcgggu acuaccagcu gcggggcgag      600

gaggaggacg agaacccgac caagcgcgag gaguucugcg agcugaaggu gguggcgguc      660

gaggccgccg agaaggggaa gggcgacgac aucugguaca acgugcaccu cgagaacggc      720

ugcaucuacc ggcgcuccag caagaucucc cuggacgacu ggguggggaa gacgaagcag      780

uucaucauca ccaccgagua cgagaaggac ggcgucaccc ccaagaagga caaggacggg      840

aaggagaagc ggagcuaccg cgcccccaag gaggacgacu ggggccugcg caagaagcgg      900

acggagaccg accucgaggc cuccgggaag accgugggcg cguucaucua cgagcaccuc      960

cuggccgagc ccuucgacaa gauccgcggc aacuucaucc acaccaucga gcggaaguac     1020

uaccgcgccg agcuggaggc cauccugacg gagcagcgga aguaccacga ggagcugcgc     1080

agcgagcaga ugcucgccgu gugcgcggag gagcuguacc gguccaaccu gccgcaccag     1140

cagagccucc ugaagaagga cauggucuac cugcuccugu ccgaccugau cuucuaccag     1200

cgcccccuca agagcaagaa gucccugauc gccgacugcc ccuacgagua cuaccgguac     1260

guggacaagg agaccgggga gaucaagaag cagggcauca agugcaucgc caagagcaac     1320

cccuacuacc aggaguuccg ccuguggcag uucgugcuca accugcggcu guucgaccgc     1380

augaccgaca aggaggucac cgccgacuac cucccgacga aggaggacua cgugcggcug     1440

uucgacuacc ugaacaaccg caaggagauc aaccaggaga cccugcucaa ggacuucuuc     1500

cggcugaaga aggugaagcu ggggcgcgag aaggaguucg agauccggug gaacuacauc     1560

gaggacaagg agaagaagua ccccugcaac gagacccgcu acgagcuccu ggucgcccug     1620

gaccgggcgg gcaucgaccg cagcuggauc gacgaggacg ggcgccagua ccggcucugg     1680

caccuccugu acuccaucga ggugaaggcc gaggccgaga gcgcccugcg caagcugaac     1740

uccgacgacg ccuucgugag cuccuuccug aaggucaagc ccuucaagaa ggacuacggc     1800

gcguacagcg agaaggccgu gaagaagcuc cuggccguca ugcggauggg cuccaugugg     1860

agcgaggcca acaucugcga ggagacccag aaccgcaucc gguccaucau ccgcggggac     1920

aucgaggaga agaucaagga gcggaugggc cacacggcgc gcagcuucaa ccagaucgac     1980

gacuuccagg cccugcccga guggcucgcc ugcuacgugg uguacgggcg gcacuccgag     2040

gucaccgaca uccagcacug ggagaccccg gagcagcugc agagcuacau caacggcuuc     2100

aagcagcacu cccugcgcaa ccccaucgug gagcagugca uccucgagac ccugcggacg     2160

gugcacgaca ucuggaagga ggccgggcac aucgacgaga uccacgucga gcugggccgc     2220

gacaugaagc ggaccgccga ccagcgcgcg cggaugacca agaacauccu caagaacgag     2280

aacaccaacc ugcgcaucaa gagccugcuc auggagcuga agaaggacac ggacaucaag     2340

gaggugcggc ccuacucccc caugcagcag gagauccugc gcaucuacga ggagggcgcc     2400

cuccugcagc ugaagcggga cgacgaggcc uucgccgaca ucagcaagau cucccagaug     2460

gcccagccga ccgcgaccga gcuccgccgg uacaagcugu ggcuggacca gaaguaccgc     2520

agccccuaca ccgggaaguu caucagccuc uccaagcugu ucacgagcgc cuaccagauc     2580

gagcacguga ucccccaguc ccgcuacuuc gacgacagcu ucuccaacaa ggucaucugc     2640

gaggccgagg ugaaccggcu caagagcaac augcugggcu acgaguucau caaggcccac     2700

gggggcgaga ucgugcacug caccaugcug ggcgacguca agauccuguc cgagagcgag     2760

uacaaggccu ucgugaacga gcacuacgug aaccaccgcg cgaagaagga gaagcuccug     2820

auggaggaca ucccccagga guuccugaac cggcagauga acgacucccg cuacaucagc     2880

aaggucguga aggcccuccu guccaacauc gugcggaccg aggacgagau ggacgucacc     2940

agcaaguucg uggugccgug cuccgggggc aucaccgacc gccugaagaa ggacuggggg     3000

cucaacgacg ucuggaacag caucguguac ccccgguucg agcgccugaa ccggaugacg     3060

ggcaccgagg ccuucgggca cugggagaac aaggagggca agcgcguguu ccagaccucc     3120

guccccaucg agcugcagcu cggcuucagc aagaagcgga ucgaccaccg ccaccacgcc     3180

auggacgcgc uggugaucgc cugcgccucc cggaacauca ucaacuaccu gaacaacgag     3240

agcgccaaca gccagaagaa gcgcgaggac cuccggcgcc ugcugugcga caagaaccgg     3300

aucauccgca agcccuggga guccuucacc caggacgccc ggguggcgcu ccaggacauc     3360

gucgugagcu ucaagaacua cgugcgcauc aucaacaagg ccacgaacua cuacgagcgc     3420

uacgacgagc aggggaagaa gaccaccgcc gagcagaagu ccagcgagau gugggccauc     3480

cggaagccga ugcacaagga gaccgucuuc ggccacguga accugaagcg caagacgacc     3540

gugaagcuga agaacgccau cgagaacauc cccaacaucu gcaacaagga gcucaaggcg     3600

uacaucaacg agcuggucga gaagcacuuc aacaccaagc agaugcuggc ccacuucaag     3660

uccaucaacu accgguggaa ccgccagaac guggagaagg uggaggucug gcaguacagc     3720

gacgagaagg agcggauggc cgccacccgc aagccccucg acacguccuu cgacaagaag     3780

cggaucgaga ccaucaccga caccgggauc cagaagaucc ugcugaacua ccucgaggag     3840

aagggcgggg acccgaacau cgccuucacg cccgagggca ucgcggagau gaacaagaac     3900

aucagccugu ucaacgacgg caagcugcac cagcccaucc ucaaggugcg cguggccaag     3960

accauggggg ccaaguacca ggucggccag accgggaaca aggccgcgaa guacgugggc     4020

gcccaggagg ggaccaaccu guacuucgcc aucuacgaga acgaggaggg cgagcggucc     4080

uacaacgccg ugccccugaa cgaggucgcc gagcgccuca agcagggcau gagcccggug     4140

cccgagaaga acgagaagga cgugccccug aaguucuacc ugucccccaa cgaccucguc     4200

uacgugccga cggaggagga caagcugagc aacgagugca agcuggacaa cgagcggauc     4260

uacaagaugg uguccgcgac cgggaagaag ugccacuucc uccccuacag cgucgccaac     4320

aucaucuaca acaaggugga guucgagucc augaacaaga ugggcaaggc ccugagcggg     4380

gagaugauca aggccgugug cuggaagcug gaggucgacc gccucggcac cauccugcgg     4440

gugaucaagu ga                                                         4452


<210>  2239
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2239
augaagaccg agcuggaccu caacaaggac cgccuguaca agcacgugcu gggccucgac       60

cuggggguca ccagcaucgg cuucgcccac gugguggagu ccgagaaccg gaacgagagc      120

gagaucaagc agaucggggu ccgcgugauc cagcuggaca acuucgacaa gguggacaag      180

uccggcaaga cgagcgaguc caagaacccc gccgucgacu ucgcggccgg gaagggccuc      240

accccgaacg gcagccggac caagaagcgc ggggcccggc gcaaccugga ccgguaccag      300

gaccgccggg acaaccugcu cgaggugcug accaaguccg gcuucaucaa cagcgagacg      360

aagcgcaccg aggacgggaa gcacaccacc caccagacgu gggagauccg ggccaaggcc      420

guguccgaga aggucgagaa ggaggaguuc gcgcgcaucc ugcucgccau caacaagaag      480

cggggcuaca agagcucccg caaggccaag aacgaggacg aggggcaggc cguggacggc      540

auggcggugg ccaagcggcu guacgaggag aaccugaccc ccggccagua cgccuacgag      600

guccucagcg ggggcaagaa guccaucccc gacuucuacc gcagcgaccu gcaggccgag      660

uucgacaaga ucuggaacuu ccagaagcag uucuacccca acauccugac cgacgaguuc      720

uacgaggagc ucaaggggaa gggccagcgg gccaccuccg cggccuucug gaagauccac      780

caguucaaca cggccgacau caaggaccug gaggacgagc ugaagaacga gcgcaccauc      840

aagcucagca agcgggacca gaagaagcug caggccuaca aguggcgcuc cgaggccgug      900

gugaagcagc ugggcaagga ggaggucgcg uacgugcuca ccgagaucaa caacaaccug      960

aacaacagcu ccggguaccu gggcgccauc agcgaccgga gcaaggagcu cuacuucaac     1020

aagcagaccg uggggcaguu ccuguacaag cagcugaagg agaacccgca cacgcgccuc     1080

aagaaccagg ucuucuaccg ccaggacuac cuggacgagu ucgagaccau cugggagaug     1140

caggcccagu uccaccccga gcugacccag gagcucaagg aggagauccg ggacaucauc     1200

aucuucuacc agcgcaagcu gaagucccag aagggccuga ucagcuucug cgagcucgag     1260

uccaaggagg uggugaucaa cgacaccaag aagaucaucg ggcugcgggu cgcccccaag     1320

agcagccccc uguuccagga guucaagauc uggcagaacc ucaacaacgu gcuggugcgc     1380

aagaagggca gccggaagcg cauggucaag gcccagaacc agcacucccu guucgaggag     1440

ccgaaggaga ucuucagccu cgaccuggcg uccaaggaga agcuguucga ggagcucaac     1500

cugaagggca accugagcgc caagaacugc cucgacaucc uggggcugaa cuccaacgag     1560

ugggagcuga acuacacgca gcucgagggc aaccggacca acaagagccu guacgaggcc     1620

uuccugaaga uccucgacau cgagggguac gacgugaagg accugcugaa ggucaagucc     1680

agcaaggacg agguggagcu cgacgaccug aaggugcccg ccuccgagau caagaacaug     1740

guccgcgaga ucuucagcac caacaagauc gacaccgaga uccuggacuu cgacgccgag     1800

aucgacggca aggcguucga gaagcagucc agcuaccagc ucuggcaccu ccuguacucc     1860

uacgaggggg acgacagcca gagcggcaac gagaagcugu acgagcugcu ggccaagaag     1920

uucggcuuca agcgggagca cgcccagauc cucgccaacg ugucccuggu ggacgacuac     1980

gggaaccuga gcuccaaggc gaugcgcaag aucuaccccc acauccggga gaacaacuac     2040

agcacggccu gcgagcucgu cggcuaccgc cacuccaagc acagccugac caaggacgag     2100

cugaaggacc gggugcucga ggaccgccug gagcugcuca agaagaacuc ccugcggcag     2160

cccguggucg agaagauccu gaaccagaug aucaacgccg ugaacgcccu caucgagaag     2220

aacagcgagc gcgacgagaa cgggaacauc accaaguacu ucaaguucga cgagauccgg     2280

aucgagcugg cccgcgagcu gaagaagaac gcggccgagc gggccgagau gaccacgaac     2340

aucaacgccg ccaagcucgu gcacgagaag aucuucaaga uccugcagaa cgaguucggc     2400

aucaagaacc cgacccgcaa cgacaucauc cgcuaccggc uguacgagga gcucaagggg     2460

aacggcuaca aggaccugua cuccaacacc uacauccccc gcgagauccu guucagcaac     2520

cagaucgaca ucgaccacau ccucccccag ucccggcugu ucgacgacag cuucuccaac     2580

aaggucgugg uguuccgcaa ggacaaccug aagaagggca accagaccgc gaucgacuac     2640

aucgggagcg aggucggcga ggaggggcuc aagaacuuca ucgagcgggu ggagucccug     2700

uacgagcugg gcaagaagaa caaggaggag ggcaucagca aggccaagua ccagaagcuc     2760

cugaaguccg ccagcgagau cggggacggc uucaucgagc gcgaccugcg ggagagccag     2820

uacaucgcca agaaggccaa ggagcuccug uucaagguga cgcgcuccgu cgugagcacc     2880

uccgggagca ucaccgaccg gcugcgcgag gacuggggcc ucaucaacgu gaugaaggag     2940

cugaaccugc ccaaguacaa gucccucggg cugaccgaga uccaggagcg gcgcaacggc     3000

caccaggucg aggugaucaa ggacuggacg aagcggaacg accaccgcca ccacgcgaug     3060

gacgcccugg ccguggccuu caccaagcac aaccacaucc aguaccucaa cuaccugaac     3120

gcgcggaaga acgagagcca caagcugcac ggcaacauca ucgccaucga gacccucgag     3180

accgagguca agaucgacga ccuggggaac aagaagcgcg uguucaagga gccgaucccc     3240

aacuuccgcc agguggccaa ggagcaccug gagaacgucc ucaucuccca caaggccaag     3300

aacaaggugg ugaccaagaa caagaacaag aucagcggca aggagaagcu gcagaucacg     3360

cugacgcccc gggggcagcu ccacaaggag accgucuacg gcaaguaccg guucuaccag     3420

uccaaggacg agaaggugag cgccaaguuc gaccuggaga ccaucgagaa gguggcgaac     3480

cccuccuaca aggccgcccu gcuccagcgc cugaacgaga acgagaacga cccgaagaag     3540

gccuucagcg ggaagaacgc ccuguccaag aaccccaucu acaucagcau ggagaacgac     3600

aucaaggucc ccgacaccgu gaagcucgug uggcuggagg aggacuacuc cauccggaag     3660

gacgucaccc ccgagaacuu caaggacgag aaguccaucg agaagauccu ggacgagggc     3720

gugaagcgca uccucaagaa ccgccugaag gaguucggcg gggacgcgaa ggaggccuuc     3780

uccgaccugg acaagaaccc gaucuggcuc aacgaggaga agggcaccag caucaagcgg     3840

gugcgcaucu ccggggucaa gaacgccgag ccccugcacu acaagaagga ccacuucggc     3900

aacgacaucc uggacgggaa cggcaacaag auccccgccg acuucgugag caccggcaac     3960

aaccaccacg uggccaucua caaggagccc gaguucgaca agaaggggca gccgguccuc     4020

gacgagcugg gcaaccagaa guacaaccug caggagaagg uggugagcuu cuucgaggcg     4080

gucgagcggg ugaaccaggg gcuccccauc aucgacaaga cguacaacca gcaccugggc     4140

uggcaguucu ucuucaccau gaagcagaac gagauguucg uguuccccaa cgagaagacc     4200

cgcuucaacc cgaucgagau cgaccugcuc gaccccaaca acaagaagcu gaucucccug     4260

aaccucuucc ggguccagaa gaucgccaac aagaacuaca uguuccgcca ccaccuggag     4320

accaacgugg acgacaagaa ggagaugaag aacaucgccu ucauccacau ccagagcugc     4380

aaccugcuca aggagaucau caaggugcgg cugaaccacc ugggggacau cgucuccgug     4440

ggcgaguacu ga                                                         4452


<210>  2240
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2240
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc caaggugucc       60

gucgacgacc acgggaacua ccugcacgac aucaagcucg gcagccgcau cauccccaug      120

ucccaggaca uccuggggaa cuucgacaag ggcgugacgg agagccagac cgccauccgg      180

accggcuucc gcuccgugcg gcgcauccgg gagcgcagcc ugcuccggcg cgagcggcug      240

caccgcgucc ugcacgugau gggguuccuc ccgccccacu acgacuccgc gaucggcugg      300

aaccgggagg aggccagcac cuacgggaag uuccugaacc acggcgagcc caagcuggcc      360

ugggagcgcc ucgaggacgg guccaugcgg uuccuguuca uggacagcuu ccacgagaug      420

auggccaacu ucgcccgcca ccagcccgcg cugguggccg acggccggaa gaucccgcuc      480

gacuggacgc uguacuaccu gcgcaagaag gcccucuccc aggccgucgg caaggaggag      540

cuggcgugga uccugcucaa cuucaaccag aagcgggggu acugccagcu gcgcggcgag      600

gaggaggagg agacccccac caagcgggag gaguacuacg agcugaaggu ggugagcguc      660

gaggccgccg aggaggggaa gggcggcaac accugguacc gcgugcagcu cgagaacggg      720

uggaucuacc ggcgccccuc caagaucgcc cuggacgacu gggugggcaa gacgaagcag      780

uucaucguca ccaccgagua cgagaaggac gggaccacgc ugaagaagga caaggacggc      840

gaggugaagc gcagcuuccg ggcccccggg gagaacgacu ggggccuccg caagaagcgg      900

accgaguccc agcuggaggc gagcggcaag accguggggg ccuucaucua cgaccaccuc      960

cucgccgagc cggccgacaa gauccgcggc aacuucaucc ggaccaucga gcgcaaguac     1020

uacaagcagg agcuggaggc cauccugcgg gagcagacga agcaccacga ggagcuccgc     1080

uccaaggaga ugcuggcgaa gugcgccgag gagcuguacc agagcaacca gccccaccgg     1140

aagucccucc ugaacaagga caugaccuac cugcuccuga ccgaccugau cuucuaccag     1200

cgcccccuca agagcaagaa gucccugauc gccgagugcc ccuacgagua cuacaaguac     1260

aaggacaagg agaaggggaa gaagaagcgg cagggcauca agugcgucgc caagagcaac     1320

ccguacuacc aggaguuccg ccuguggcag uucaucguga accuccggcu guucgaccgc     1380

accgacgaca aggaggugac ggccgaguac cugcggagcc gcgaggacua cgcgcggcug     1440

uucgccuacc ugaacgaccg caaggagauc aaccaggaga cccugcugaa ggacuucuuc     1500

gggcucaaga aggucaagau cggcgccgag aaggaguucg ccauccggug gaacuacauc     1560

gaggacaagg agaagaagua ccccugcaac gagacccgcc acgagcugcu gcucgcccug     1620

gaccgcgcgg gcauggaccg guccuggcug gacgacagcg agcuccagua ccgccugugg     1680

caccuccugu acuccgugga ggcccgggac gaggccgagg gggcccugcg caagcugcgg     1740

gacgacgacg aguucguggg cgcguuccuc aaggucaagc ccuucaagaa ggacuacggg     1800

gccuacagcg agaaggccgu gaagaagcug cuggccguga ugcgcauggg cuccuggugg     1860

cacgaggagg acaucugcaa gcagacccgg gaccgcaucg agcggaucgu ccagggggac     1920

aucgaggaga agauccgcga gcggaugggc cacgccgcgu acagcuucuc ccgccucgcc     1980

gacuuccagg gccugcccga guggcuggcc ugcuacgugg ucuacgggcg gcacagcgag     2040

gccacgcaca uccuccgcug ggacuccccg gacaagcugc ugagcuacau caagggcuuc     2100

cggcagcacu cccuccgcaa ccccaucgug gagcagugca uccuggagac ccugcggacc     2160

gugcacgaca ucuggaagga ggccgggcac aucgacgaga uccacgucga gcucggccgc     2220

agcaugaagu ccaccgcgga gcagcgggcc cacaugacga agaacauccu gcgcaacgag     2280

aacaccaacc ugcggaucaa gugccuccug auggagcuga agaacgacgc cgacgugaag     2340

gacgugcgcc ccuacagccc caugcagcag gagauccucc gcaucuacga ggagggcgcc     2400

cugcagcagc ugaccaagga cgacgccgag uacgcggaga ucuccaagau cagcaagaug     2460

gcccagccgu ccgccaagga gcuccugcgg uacaagcugu ggcucgagca gaaguaccgc     2520

agccccuaca ccgggaagcc caucagccug uccaagcugu ucacgagcgc cuaccagauc     2580

gagcacguca ucccccaguc ccgguacuuc gacgacagcu ucaacaacaa ggugaucugc     2640

gaguccgagg ugaacacccu caagagcaac augcugggcu acgaguucau caagaaccac     2700

ugcggggaga ucguccacug caccaugcug ggcgacguga agguguucuc cgagagcgag     2760

uacaaggcgu ucgucaccga gcacuacucc aacaacaagu acaagaagga gaagcuccug     2820

cuggaggaca ucccgcagga guuccucaac cgccagauga acgacagccg guacaucucc     2880

aaggugguga aggggcugcu gagcaacguc gugcgcacgg aggacgagcu cgaggccacc     2940

uccaaguucg uggucagcug caccggcggc aucaccgacc ggcugaagaa ggacuggggg     3000

cugaacgacg uguggaacuc caucguguac agccgcuucg agcggcucaa cggccugacc     3060

aaguccgagc acuucgggca cugggagaac aaggagggca agcgcgucuu ccagacgagc     3120

gugcccaugg agcugcagcg gggguucaac aagaagcgca ucgaccaccg gcaccacgcc     3180

auggacgccc ucgugaucgc cugcgcgagc cgcaacauca ucaacuaccu gaacaacgag     3240

uucgccaagu cccccaagca gcgggaggac cugcgccagc uccugugcga caagaaccgg     3300

aucauccgca agcccuggga gaccuucacc caggacgccc gggccgcccu ggaggacguc     3360

guggugagcu ucaagaacua cguccgcgug aucaacaaga ccacgaacua cuacgagcgc     3420

uacgacgcgg acggcaagaa gaccauggug ccgcagaagg gcaacgagau gugggccguc     3480

cggaagccca ugcacgaggg gaccauguac ggccgcguga accuccggcg caaggccgug     3540

gucaagcuga aggaggcccu ggagaacauc uccagcaucu gcaacaagga gcuccgggac     3600

uacaucaacg ugcuggugga gaagcaguuc aacaccaagc agcugcucgc ccacuucaag     3660

uccauggacu accgcuggaa caagcagcac aucgacaagg ucgaggugug gcgguucagc     3720

gacgagaagg ugcagauggu cgcggcccgc aagccccugg acacguccuu cgacaaggag     3780

ugcaucgcca gcaucaccga caccgggauc cagaagaucc ugcucaacua ccuggagucc     3840

aagggcgggg acccggccau cgcguucacc agcgagggca ucgccgagau gaaccagaac     3900

aucgccgugu acaacgacgg caagaagcac cagcccguga ucaagguccg gaaguccacg     3960

aagcugggga agaaguucgc cgugggccag aucgggugca agagcaagca guucgugguc     4020

gccgaggcgg gcaccaaccu cuacuucgcc aucuacgagg acgaggaggg gaagcgcucc     4080

uacgacacca ucccccugaa cgaggugacc gagcggcuga agcagggccu cagccccgug     4140

ccggagaaga acgagaagga ggucccccug aaguucuacc ugucccccaa cgaccucgug     4200

uacgugccca cggaggacga gaugcagaug aaggagugca agcugaacaa ggagcgcauc     4260

uacaagaugg ucagcuccac cggcaacgag ugccacuucc ugccguacag cguggccucc     4320

gucaucuaca acaaggugga guucgagagc augaacaaga uggggcgggc ccucaccggc     4380

gagaugauca aggccgugug cuggaagcug gacgucgacc gccuggggaa cauccuccgg     4440

gugaucaagu ga                                                         4452


<210>  2241
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2241
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc caagaucucc       60

aucgacgaca acgggaacua ccugcgcgac auccagcucg gcagccggau cauccccaug      120

ucccaggacg ugcuggggaa cuucgacaac ggcgucacgg agagccagac cgccgugcgc      180

accggcuucc gggggcugcg ccggauccgc gagcgguccc uccugcgccg ggagcgccug      240

caccgggugc uccacgucau gggcuuccug ccgccccacu acgacagcgc gaucgggugg      300

aacaaggagg agcccaccac guacggcaag uuccugaacc acggggagcc caagcucgcc      360

ugggagcgcc agggcgacgg cuccaugcgg uuccuguuca uggacagcuu ccacaagaug      420

auggaggacu ucgccccgca ccagcccgcc cugguggccg acgggcgcaa gaucccccuc      480

gacuggaccc uguacuaccu gcggaagaag gcgcucaccc agcccaucaa gaaggaggag      540

cuggccugga uccugcucaa cuucaaccag aagcgcggcu acuaccagcu gcgcggggag      600

gaggaggagg agaacccgac caagcgggag gaguaccacg agcugaaggu ggucggcgug      660

gaggccgccg aggagggcaa gggcgggaac acgugguaca acgugaagcu cgagaacggg      720

uggaucuacc ggcgcuccag caagaucccc cuggacgacu gggucggcaa gacccggccc      780

uucaucguga ccaccgagua cgaggcggac gggaucacgc ugaagaagaa caaggagggc      840

gaggugaagc gcuccuuccg cgcccccggc gaggacgacu gggggcucug gaagaagcgg      900

accgagagcc agcuggaggc cuccggcaag accgucgggg ccuucaucua cgaccaccuc      960

cuggccgagc cggcggacaa gauccgcggc aacuucaucc ggaccaucga gcgcaaguac     1020

uacaagcagg agcuggccga cauccugcgg gagcagagca aguaccacga ggagcuccag     1080

uccaagaaga uccuggccga cugcgccgag gagcuguacc gcagcaacca gccccaccgg     1140

gacucccucc ugaagaagga caugguguac cugcuccuga cggaccugau cuucuaccag     1200

cgcccccuca agagcaagaa gucccugauc gccgacugcc ccuacgagua cuacgaguac     1260

guggacaagg agaccgggga gaucaagcgg cagggcauca agugcaucgc caagagcaac     1320

ccguacuacc aggaguuccg ccuguggcag uucguccuca accugcggcu guacgaccgc     1380

accgaggaca aggaggugac cgcggaguac cuccccagca aggaggacua cgcccggcug     1440

uucguguacc ugaacgaccg caaggagauc aaccaggaga cgcugcucaa ggacuucuuc     1500

aagcugaaga aggucaagca gcgguccgag aaggacuucc ccgugcgcug gaacuacauc     1560

gaggacaagg agaagaagua ccccugcaac gagacccggc acgagcugcu cauggcccug     1620

gaccgcgccg gcaucgagcg gagcuggcug gacgacaccg cgcgccagua ccgccucugg     1680

caccuccugu acuccaucga ggugaaggac gaggccgagc gggcccugcg caagcugaag     1740

gacgacgacg aguucgucag cgccuuccug aaggugaagc cguucaagaa ggacuacggg     1800

gccuacuccg agaaggcggu gaagaagcuc cuggccguca ugcggauggg cagccacugg     1860

ugcgaggacg acaucugcaa ggagaccaag gagcgcaucg agcacauccu gcagggggac     1920

gugggcgaga agaucaagga gcggaugaac cacacggccu acucccucgg ggagcugagc     1980

gacuuccagg cccugcccga guggcucgcg ugcuacgucg uguacggccg ccacuccgag     2040

gccaccgaca ucaagcacug gaagaccccc gagagccuga uggccuacau caacggcuuc     2100

aagcagcacu cccugcggaa ccccaucgug gagcagugca uccucgagac ccugcgcacg     2160

guccacgaca ucuggaagga ggccgggcac aucgacgaga uccacgugga gcugggccgg     2220

agcaugaagu ccaccagcga ccagcgcgcc cggaugaccg agaacauccu ccgcaacgag     2280

aacaccaacc ugcggaucaa gucccugcuc auggagcuga agaacgacgc ggacaucaag     2340

gacgugcgcc cguacagccc caugcagcag gagauccugc ggaucuacga ggagggggcc     2400

cuccaggagc ugacgaagga ggacaacgac uacgaggaga ucuucaagau cuccaagaug     2460

ccccagccca gcgccaagga gcugcucaag uacaagcugu ggcuggagca gaaguaccgc     2520

uccccguaca ccggcaagcc caucagccuc agcaagcugu ucaccuccgc cuaccagauc     2580

gagcacauca ucccccagag ccgguacuuc gacgacuccu ucaacaacaa ggucaucugc     2640

gagagcgagg ugaacgcccu gaagggcaac augcucgggu acgaguucau caagaaccac     2700

ggcggggaga ucgugcacug cacccugcug ggcgacguca aggugcagaa cgaguccgag     2760

uacaaggcgu ucgugacgga gcacuacgcc aacaacaagu acaagaagga gaagcuccug     2820

auggaggaca ucccgcagga cuuccugaac cgccaccuca acgacagccg guacaucucc     2880

aaggucguga agacccugcu gagcaacauc gugcgcaccg aggacgagau ggagaccacc     2940

uccaaguucg ucgugagcug caccgggggc aucacggacc ggcucaagaa ggacuggggc     3000

cugaacgacg uguggaacuc caucgucuac agccgcuucg agcgccugaa cgagcucacc     3060

aaguccgacg ccuucgggcg gugggagaac aaggagggca agcgcguguu ccagaccagc     3120

gugccccugg agcugcagcg gggguucaac aagaagcgca ucgaccaccg gcaccacgcc     3180

auggacgccc ucgucaucgc gugcgccucc cgcaacauca ucaacuaccu gaacaacgag     3240

agcgccaacu cccccaagaa gcgggaggac cugcgccagc agcucugcga caagaaccgg     3300

aucauccgca agcccuggga gagcuucacg caggacgccc gggcggcccu ggaggacgug     3360

guggucagcu ucaagaacua cgugcgcgug aucaacaagg ccaccaacua cuacgagcgg     3420

uacgacgccg acggcaagaa gaccaucacc aagcagaagu cccgcgagau gugggccguc     3480

cggaagccga ugcacaagga gacgguguuc gggcacguga accugcgccg gaaggcgauc     3540

gucaagcuca aggacgcccu ggagaacauc cccagcaucu gcaacaagga gcugcgcgac     3600

uacaucaacg gccucgugga gcggaacuuc aacaccaagc agcugcacgc ccacuuccgc     3660

uccaccgacu accgcuggaa caagcagaac guggacaagg ucgaggugug gcgguucagc     3720

gacgagaagg agccccuggu ggccacccgc aagccccucg acacguccuu cgacgccaag     3780

cggaucgaca ccaucaccga caccggcauc cagaagaucc ugcugaacua ccucgaggcg     3840

aagggcgggg acccgcugau cgccuucacg cccgagggga ucgccgagau gaaccagaac     3900

aucgcccugu acaacgacgg caagaugcac cagcccaucc ucaagguccg caucagcgag     3960

cccaaggggg ccaaguacca gaucggccag accggcaaca aggcggacaa guucguggag     4020

gcccagaagg ggaccaaccu guacuucgcc aucuacgagg acgaggaccg gaaccgcacc     4080

uacaacacga ucccgcugaa cgagguggcc gagcggcuca agcagggccu gccgcccguc     4140

cccgagaaga acgagaagga ggugccccug aaguucuacc ucucccccaa cgaccuggug     4200

uacgucccca ccgaggagga gauccugaug aaggagugca agcucaacaa gaaccgcauc     4260

uacaagaugg ugagcuccac cgggaaccag ugcuucuuca uccccuuccg gguggcgguc     4320

agcaucguga acaagaugga guacuccccg cugaacaaga uggagaaggc caucagcucc     4380

gagaugauca aggccgugug cuggaagcug gaggucgacc gccucggcaa caucauccgg     4440

guggugaagu ga                                                         4452


<210>  2242
<211>  4458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2242
augaagaaca uccugggccu cgaccugggg accaacagca ucggcugggc caagaucucc       60

aucgacgaca acgggaagua caucaaggac agcaucaagc ugggcucccg caucaucccc      120

augagccagg acgugcucuc caacuucagc aaggggguca cccuguccca gacgagcgag      180

cggaccuccu uccgcggcgc ccggcgccug cgggagcgca cccuccugcg gcgcgagcgg      240

cugcaccgca uccuccaccu ccugggcuuc cucccgaagc acuacgagaa ggcgaucggg      300

ugggaccgga acgacaacaa gaccuacggc aaguucauca accacgccga gcccaagcug      360

gccuggugca aggacaacaa cgggaagaug cgcuuccugu ucauggacag cuuccacgac      420

augcucuccg aguucagcga gaagcacccc gagcugcugg ccaacggcaa gaagaucccc      480

cucgacugga cgaucuacua ccugcggaag aaggcccugu acaaggcgau caccaaggag      540

gagcucgccu ggauccugcu gaacuucaac cagaagcgcg gguacuacca gcuccggggc      600

gaggaggacc gcgacuccga ggccaacaag aaggaguacc uggagcugaa ggugaucggc      660

guggaggcca ccggggagaa gaagggcaac gacaucuggu acaacaucca ccucgagaac      720

ggguggaucu accggcgcag cuccaagaac ccgcugaacg acuggcuggg caaggucaag      780

agcuucaucg ugaccacgga ccucgacgcg gacggcaacc ccaagaagga caaggacggg      840

aacaucaagc gguccuuccg cgcccccagc gagggcgacu ggacccugau caagaagcgg      900

accgaguccc ugcucgagaa cagcgggcag accgugggcc aguucaucua caaccacauc      960

cuggaggagc ccagcgccaa gauccgcggg aacuucaucc gcacgaucga gcggaaguac     1020

uacaaggacg agcugaaggc cauccucgag aagcagaagg aguuccacca cgagcugucc     1080

agcgaguccc ugcucaccga gugcgccaag gagcuguaca agcgcaacau cgcgcaccag     1140

gagguccugc ucaagaaggg caugauccac cuccugcuga acgacauccu guucuaccag     1200

cggccgcuga agaucaagaa gucccucauc gacaacugcc ccuacgagaa cuacaaguac     1260

guggacaaga aguccggcga caucaagguc aagagcauca agugcaucgc caagagcaac     1320

cccuucuucc aggaguuccg ccuguggcag uucgugagca accugcggcu guaccgcaag     1380

uccgacgaca aggacgugac cagcgagcac cucgugacca cggacgacua cgccaaccug     1440

uucaccuacc ugaacaaccg gaaggacauc aagcaguccg uccuccugaa ggaguacuuc     1500

aagaucaaga aggugaagac caucaacggg gccgaguacc ccgugcgcug gaacuacauc     1560

gacgacgaca acaagaccua cccgugcaac gagacgcggu acgacauccu gcacgcccuc     1620

agcaagaccg agcugaaguc cgaguggcug acccccgagc gcaccuaccg gcucuggcac     1680

cuccuguaca gcaucgaguc caaggaggag acgaagaagg cgcugaacaa gcugaaccug     1740

agcgacgacu ucaccgaggc cuuccucacc aucaagcccu ucaagaagga guacggcucc     1800

uacagcgaga aggccaucaa gaagcugcug ucccucaugc gcauggggua ccuguggagc     1860

gaggacgagg ccaucaagca ggucggcgcc gagaucgaga agaccaucga gggggagcug     1920

cccgacuacg ugcuccagcg gcuggagaag ucccagcaca acuacaacaa gaucagcgac     1980

uucagcggcc ugccgcucug gcuggcgucc uacaucaucu acggccgcca cagcgaggcc     2040

aaggagauca uccacuggga caaccccgag uccaugaugg ccuacaucaa ggacuuccgg     2100

cagcacagca ugcgcaaccc caucguggag cagugcgucc uggagacgcu ccggaccgug     2160

cacgacaucu ggaaggagau cgggcacauc gacgagaucc acaucgagcu gggccgcgag     2220

augaagucca ccagcgagca gcggaaggcc aucacccaga acgugcugca caaccagaac     2280

acgaacaucc gcaucaagcu ccugcugcag gagcucaaga acgacaacaa caucaccggg     2340

guccgccccu acuccccgau gcagcaggag auccugcgga ucuacgagga gggcgcccug     2400

caggggcuca ccaaggagga caaggacuac aacgacaucc ugaagaucag ccagaccgcg     2460

cagcccacgu ccaacgagcu gacccgcuac aagcucuggc uggagcagaa guaccggagc     2520

cccuacaccg gcaaggccau cucccugagc aagcuguuca ccuccgccua ccagaucgag     2580

cacgugaucc cccagagccg cuacuucgac gacucccuga gcaacaaggu gaucugcgag     2640

agcgagguca acacccugaa gaaggccaug cucggcaugg aguucaucaa gacgugcggg     2700

ggccagaagg ugcacugcac ccugcugggg gacgucgaga ucuacaacga ggaggacuac     2760

aaggcguucg ugaacgagca cuacuccaac aaccggacca agcgcaacaa gcuccugaug     2820

gaggacaucc cgcaggaguu caucaaccgg cagcugaacg acagccgcua caucuccaag     2880

auggugaagc agcuccugag cgccaucguc cggggccagg aggaggugga ggccaccucc     2940

aaguucguga uccccugcac gggcgggauc accgaccgcc ugaagaagga cuggggccuc     3000

aacgacaucu ggaacaaccu ggucagcccc cgguuccagc gcaugaacgc ccugaccggg     3060

uccgaguacu ucggccacug ggagaacaag gaggggaagc ggguguucca gaccacgaug     3120

cccauccagc uccagcaggg cuucagcaag aagcgcaucg accaccggca ccacgccaug     3180

gacgcgcugg ugaucgccug cgccucccgc aacaucauca gcuaccugaa caacgagacc     3240

gcccaggaca ccgagcggcg ccgggaccuc cgccagaagc ugugcgacaa gggcgggucc     3300

guccggaagc cgugggacag cuucacccag gacacgcagu acgcccugaa ggacaucauc     3360

gugaccuuca agaacuacgu gcgcaucauc aacaaggcga ccaacuacua cgagcacuac     3420

aagaccgacg gcaagaaggg gaugaucucc cagaagggcg acgagcucug ggccauccgc     3480

aagcccaugc acaaggagac ggucuucggg aaggugaacc ugcggcgccc caagcgggug     3540

agccugucca aggccaucga cagcgucgac gugaucugca accgcaagcu caagaaguac     3600

aucaaggccc ggcugaacga gggccugcag aagaagcaga uccucaacau cuucaaggag     3660

gugaacaacg ucugggaggg caucgacgug aacaagaucg acaucuggga gauguccaac     3720

gacaaggagc cgaugguggc cacccgcaag cagcuggaca ccagcuucga cgagaagaag     3780

auccggucca ucagcgacac cgggauccag aagauccugc ucaacuaccu gaucacgaag     3840

aacaacaacc ccgagcuggc guucaccccc gagggcaucc aggagaugaa cagcaacauc     3900

uccgaguaca acgaccacaa gaagcaccag cccaucuaca agguccgcgu gaccgagccg     3960

cucggggcca aguaccccau cggcaccaug gggaacaacc ccaagaaguu cguggaggcc     4020

caggccggca cgaaccugua cuucgcgauc uacgaggaca aggagggcaa ccggaacuac     4080

gacaccaucc cccugaacau cgccgccgag cgccucaacc agcagcugag cccggucccc     4140

gagaccaacg agaacaacac gccccugaag uucuaccucu cccccaacga ccugguguac     4200

gugccgagcg aggaggagcg gcacuccggg aacaacaucc agcuggacaa gaaccgcauc     4260

uacaagaugg ucagcuccac cagcaagcag ugcuucuuca uccaccaccg gguggccauc     4320

ucccucgugg acaagaagga guucagcgcc cagaacaaga uggagcgcuc caucgacaac     4380

gagcagguca ugaucaagag cgugugcugg aagcugauca ccgaccggcu gggcaacauc     4440

aucaagguga ucaaguga                                                   4458


<210>  2243
<211>  4458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2243
augaagaaca uccugggccu cgaccugggg accaacagca ucggcugggc caagaucucc       60

aucgacgaca acgggaagua caucaaggac agcaucaagc ugggcucccg caucaucccc      120

augagccagg acgugcucuc caacuucagc aaggggguca cccuguccca gacgagcgag      180

cggaccuccu uccgcggcgc ccggcgccug cgggagcgca cccuccugcg gcgcgagcgg      240

cugcaccgca uccuccaccu ccugggcuuc cucccgaagc acuacgagaa ggcgaucggg      300

ugggaccgga acgacaacaa gaccuacggc aaguucauca accacgccga gcccaagcug      360

gccuggugca aggacaacaa cgggaagaug cgcuuccugu ucauggacag cuuccacgac      420

augcucuccg aguucagcga gaagcacccc gagcugcugg ccaacggcaa gaagaucccc      480

cucgacugga cgaucuacua ccugcggaag aaggcccugu acaaggcgau caccaaggag      540

gagcucgccu ggauccugcu gaacuucaac cagaagcgcg gguacuacca gcuccggggc      600

gaggaggacc gcgacuccga ggccaacaag aaggaguacc uggagcugaa ggugaucggc      660

guggaggcca ccggggagaa gaagggcaac gacaucuggu acaacaucca ccucgagaac      720

ggguggaucu accggcgcag cuccaagaac ccgcugaacg acuggcuggg caaggucaag      780

agcuucaucg ugaccacgga ccucgacgcg gacggcaacc ccaagaagga caaggacggg      840

aacaucaagc gguccuuccg cgcccccagc gagggcgacu ggacccugau caagaagcgg      900

accgaguccc ugcucgagaa cagcgggcag accgugggcc aguucaucua caaccacauc      960

cuggaggagc ccagcgccaa gauccgcggg aacuucaucc gcacgaucga gcggaaguac     1020

uacaaggacg agcugaaggc cauccucgag aagcagaagg aguuccacca cgagcugucc     1080

agcgaguccc ugcucaccga gugcgccaag gagcuguaca agcgcaacau cgcgcaccag     1140

gagguccugc ucaagaaggg caucauccac cuccugcuga acgacauccu guucuaccag     1200

cggccgcuga agaucaagaa gucccucauc gacaacugcc ccuacgagaa cuacaaguac     1260

guggacaaga aguccggcga caucaagguc aagagcauca agugcaucgc caagagcaac     1320

cccuucuucc aggaguuccg ccuguggcag uucgugagca accugcggcu guaccgcaag     1380

uccgacgaca aggacgugac cagcgagcac cucgugacca cggacgacua cgccaaccug     1440

uucaccuacc ugaacaaccg gaaggacauc aagcaguccg uccuccugaa ggaguacuuc     1500

aagaucaaga aggugaagac caucaacggg gccgaguacc ccgugcgcug gaacuacauc     1560

gacgacgaca acaagaccua cccgugcaac gagacgcggu acgacauccu gcacgcccuc     1620

agcaagaccg agcugaaguc cgaguggcug acccccgagc gcaccuaccg gcucuggcac     1680

cuccuguaca gcaucgaguc caaggaggag acgaagaagg cgcugaacaa gcugaaccug     1740

agcgacgacu ucaccgaggc cuuccucacc aucaagcccu ucaagaagga guacggcucc     1800

uacagcgaga aggccaucaa gaagcugcug ucccucaugc gcauggggua ccuguggagc     1860

gaggacgagg ccaucaagca ggucggcgcc gagaucgaga agaccaucga gggggagcug     1920

cccgacuacg ugcuccagcg gcuggagaag ucccagcaca acuacaacaa gaucagcgac     1980

uucagcggcc ugccgcucug gcuggcgucc uacaucaucu acggccgcca cagcgaggcc     2040

aaggagauca uccacuggga caaccccgag uccaugaugg ccuacaucaa ggacuuccgg     2100

cagcacagca ugcgcaaccc caucguggag cagugcgucc uggagacgcu ccggaccgug     2160

cacgacaucu ggaaggagau cgggcacauc gacgagaucc acaucgagcu gggccgcgag     2220

augaagucca ccagcgagca gcggaaggcc aucacccaga acgugcugca caaccagaac     2280

acgaacaucc gcaucaagcu ccugcugcag gagcucaaga acgacaacaa caucaccggg     2340

guccgccccu acuccccgau gcagcaggag auccugcgga ucuacgagga gggcgcccug     2400

caggggcuca ccaaggagga caaggacuac aacgacaucc ugaagaucag ccagaccgcg     2460

cagcccacgu ccaacgagcu gacccgcuac aagcucuggc uggagcagaa guaccggagc     2520

cccuacaccg gcaaggccau cucccugagc aagcuguuca ccuccgccua ccagaucgag     2580

cacgugaucc cccagagccg cuacuucgac gacucccuga gcaacaaggu gaucugcgag     2640

agcgagguca acacccugaa gaaggccaug cucggcaugg aguucaucaa gacgugcggg     2700

ggccagaagg ugcacugcac ccugcugggg gacgucgaga ucuacaacga ggaggacuac     2760

aaggcguucg ugaacgagca cuacuccaac aaccggacca agcgcaacaa gcuccugaug     2820

gaggacaucc cgcaggaguu caucaaccgg cagcugaacg acagccgcua caucuccaag     2880

auggugaagc agcuccugag cgccaucguc cggggccagg aggaggugga ggccaccucc     2940

aaguucguga uccccugcac gggcgggauc accgaccgcc ugaagaagga cuggggccuc     3000

aacgacaucu ggaacaaccu ggucagcccc cgguuccagc gcaugaacgc ccugaccggg     3060

uccgaguacu ucggccacug ggagaacaag gaggggaagc ggguguucca gaccacgaug     3120

cccauccagc uccagcaggg cuucagcaag aagcgcaucg accaccggca ccacgccaug     3180

gacgcgcugg ugaucgccug cgccucccgc aacaucauca gcuaccugaa caacgagacc     3240

gcccaggaca ccgagcggcg ccgggaccuc cgccagaagc ugugcgacaa gggcgggucc     3300

guccggaagc cgugggacag cuucacccag gacacgcagu acgcccugaa ggacaucauc     3360

gugaccuuca agaacuacgu gcgcaucauc aacaaggcga ccaacuacua cgagcacuac     3420

aagaccgacg gcaagaaggg gaugaucucc cagaagggcg acgagcucug ggccauccgc     3480

aagcccaugc acaaggagac ggucuucggg aaggugaacc ugcggcgccc caagcgggug     3540

agccugucca aggccaucga cagcgucgac aucaucugca accgcaagcu caagaaguac     3600

aucaaggccc ggcugaacga gggccugcag aagaagcaga uccucaacau cuucaaggag     3660

cugaacaacg ugugggaggg caucgacgug aacaagaucg acaucuggga gauguccaac     3720

gacaaggagc cgauggucgc cacccgcaag cagcuggaca ccagcuucga cgagaagaag     3780

auccggucca ucagcgacac cgggauccag aagauccucc ugaacuaccu gaucacgaag     3840

aacaacaacc ccgagcucgc guucaccccc gagggcaucc aggagaugaa cagcaacauc     3900

uccgaguaca acgaccacaa gaagcaccag cccaucuaca aggugcgcgu gaccgagccg     3960

cugggggcca aguaccccau cggcaccaug gggaacaacc ccaagaaguu cgucgaggcc     4020

caggccggca cgaaccugua cuucgcgauc uacgaggaca aggagggcaa gcggaacuac     4080

gacaccaucc cccucaacau cgccgccgag cgccugaacc agcagcugag cccggugccc     4140

gagaccaacg agaacaacac gccccucaag uucuaccugu cccccaacga ccugguguac     4200

gucccgagcg aggaggagcg gcacuccggg aacaacaucc agcucgacaa gaaccgcauc     4260

uacaagaugg ugagcuccac cagcaagcgg ugcuucuuca uccaccaccg cguggccauc     4320

ucccuggucg acaagaagga guucagcgcc cagaacaaga uggagcgguc caucgacaac     4380

gagcagguca ugaucaagag cgugugcugg aagcugauca ccgaccgccu cggcaacauc     4440

aucaagguga ucaaguga                                                   4458


<210>  2244
<211>  4458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2244
augaagaaca uccugggccu cgaccugggg accaacagca ucggcugggc caagaucucc       60

aucgacgaca acgggaagua caucaaggac agcaucaagc ugggcucccg caucaucccc      120

augagccagg acgugcucuc caacuucagc aaggggguca cccuguccca gacgagcgag      180

cggaccuccu uccgcggcgc ccggcgccug cgggagcgca cccuccugcg gcgcgagcgg      240

cugcaccgca uccuccaccu ccugggcuuc cucccgaagc acuacgagaa ggcgaucggg      300

ugggaccgga acgacaacaa gaccuacggc aaguucauca accacgccga gcccaagcug      360

gccuggugca aggacaacaa cgggaagaug cgcuuccugu ucauggacag cuuccacgac      420

augcucuccg aguucagcga gaagcacccc gagcugcugg ccaacggcaa gaagaucccc      480

cucgacugga cgaucuacua ccugcggaag aaggcccugu acaaggcgau caccaaggag      540

gagcucgccu ggauccugcu gaacuucaac cagaagcgcg gguacuacca gcuccggggc      600

gaggaggacc gcgacuccga ggccaacaag aaggaguacc uggagcugaa ggugaucggc      660

guggaggcca ccggggagaa gaagggcaac gacaucuggu acaacaucca ccucgagaac      720

ggguggaucu accggcgcag cuccaagaac ccgcugaacg acuggcuggg caaggucaag      780

agcuucaucg ugaccacgga ccucgacgcg gacggcaacc ccaagaagga caaggacggg      840

aacaucaagc gguccuuccg cgcccccagc gagggcgacu ggacccugau caagaagcgg      900

accgaguccc ugcucgagaa cagcgggcag accgugggcc aguucaucua caaccacauc      960

cuggaggagc ccagcgccaa gauccgcggg aacuucaucc gcacgaucga gcggaaguac     1020

uacaaggacg agcugaaggc cauccucgag aagcagaagg aguuccacca cgagcugucc     1080

agcgaguccc ugcucaccga gugcgccaag gagcuguaca agcgcaacau cgcgcaccag     1140

gagguccugc ucaagaaggg caugauccac cuccugcuga acgacauccu guucuaccag     1200

cggccgcuga agaucaagaa gucccucauc gacaacugcc ccuacgagaa cuacaaguac     1260

guggacaaga aguccggcga caucaagguc aagagcauca agugcaucgc caagagcaac     1320

cccuucuucc aggaguuccg ccuguggcag uucgugagca accugcggcu guaccgcaag     1380

uccgacgaca aggacgugac cagcgagcac cucgugacca cggacgacua cgccaaccug     1440

uucaccuacc ugaacaaccg gaaggacauc aagcaguccg uccuccugaa ggaguacuuc     1500

aagaucaaga aggugaagac caucaacggg gccgaguacc ccgugcgcug gaacuacauc     1560

gacgacgaca acaagaccua cccgugcaac gagacgcggu acgacauccu gcacgcccuc     1620

agcaagaccg agcugaaguc cgaguggcug acccccgagc gcaccuaccg gcucuggcac     1680

cuccuguaca gcaucgaguc caaggaggag acgaagaagg cgcugaacaa gcugaaccug     1740

agcgacgacu ucaccgaggc cuuccucacc aucaagcccu ucaagaagga guacggcucc     1800

uacagcgaga aggccaucaa gaagcugcug ucccucaugc gcauggggua ccuguggagc     1860

gaggacgagg ccaucaagca ggucggcgcc gagaucgaga agaccaucga gggggagcug     1920

cccgacuacg ugcuccagcg gcuggagaag ucccagcaca acuacaacaa gaucagcgac     1980

uucagcggcc ugccgcucug gcuggcgucc uacaucaucu acggccgcca cagcgaggcc     2040

aaggagauca uccacuggga caaccccgag uccaugaugg ccuacaucaa ggacuuccgg     2100

cagcacagca ugcgcaaccc caucguggag cagugcgucc uggagacgcu ccggaccgug     2160

cacgacaucu ggaaggagau cgggcacauc gacgagaucc acaucgagcu gggccgcgag     2220

augaagucca ccagcgagca gcggaaggcc aucacccaga acgugcugca caaccagaac     2280

acgaacaucc gcaucaagcu ccugcugcag gagcucaaga acgacaacaa caucaccggg     2340

guccgccccu acuccccgau gcagcaggag auccugcgga ucuacgagga gggcgcccug     2400

caggggcuca ccaaggagga caaggacuac aacgacaucc ugaagaucag ccagaccgcg     2460

cagcccacgu ccaacgagcu gacccgcuac aagcucuggc uggagcagaa guaccggagc     2520

cccuacaccg gcaaggccau cucccugagc aagcuguuca ccuccgccua ccagaucgag     2580

cacgugaucc cccagagccg cuacuucgac gacucccuga gcaacaaggu gaucugcgag     2640

agcgagguca acacccugaa gaaggccaug cucggcaugg aguucaucaa gacgugcggg     2700

ggccagaagg ugcacugcac ccugcugggg gacgucgaga ucuacaacga ggaggacuac     2760

aaggcguucg ugaacgagca cuacuccaac aaccggacca agcgcaacaa gcuccugaug     2820

gaggacaucc cgcaggaguu caucaaccgg cagcugaacg acagccgcua caucuccaag     2880

auggugaagc agcuccugag cgccaucguc cggggccagg aggaggugga ggccaccucc     2940

aaguucguga uccccugcac gggcgggauc accgaccgcc ugaagaagga cuggggccuc     3000

aacgacaucu ggaacaaccu ggucagcccc cgguuccagc gcaugaacgc ccugaccggg     3060

uccgaguacu ucggccacug ggagaacaag gaggggaagc ggguguucca gaccacgaug     3120

cccauccagc uccagcaggg cuucagcaag aagcgcaucg accaccggca ccacgccaug     3180

gacgcgcugg ugaucgccug cgccucccgc aacaucauca gcuaccugaa caacgagacc     3240

gcccaggaca ccgagcggcg ccgggaccuc cgccagaagc ugugcgacaa gggcgggucc     3300

guccggaagc cgugggacag cuucacccag gacacgcagu acgcccugaa ggacaucauc     3360

gugaccuuca agaacuacgu gcgcaucauc aacaaggcga ccaacuacua cgagcacuac     3420

aagaccgacg gcaagaaggg gaugaucucc cagaagggcg acgagcucug ggccauccgc     3480

aagcccaugc acaaggagac ggucuucggg aaggugaacc ugcggcgccc caagcgggug     3540

agccugucca aggccaucga cagcgucgac gugaucugca accgcaagcu caagaaguac     3600

aucaaggccc ggcugaacga gggccugcag aagaagcaga uccucaacau cuucaaggag     3660

cugaacaacg ugugggaggg caucgacguc aacaagaucg acaucuggga gauguccaac     3720

gacaaggagc cgaugguggc cacccgcaag cagcuggaca ccagcuucga cgagaagaag     3780

auccggucca ucagcgacac cgggauccag aagauccucc ugaacuaccu gaucacgaag     3840

aacaacaacc ccgagcucgc guucaccccc gagggcaucc aggagaugaa cagcaacauc     3900

uccgaguaca acgaccacaa ggagcaccag cccaucuaca aggugcgcgu caccgagccg     3960

cugggggcca aguaccccau cggcaccaug gggaacaagc ccaagaaguu cguggaggcc     4020

caggccggca cgaaccugua cuucgcgauc uacgaggaca aggagggcaa ccggaacuac     4080

gacaccaucc cccucaacau cgccgccgag cgccugaacc agcagcugag cccggugccc     4140

gagaccaacg agaacaacac gccccucaag uucuaccugu cccccaacga ccuggucuac     4200

gugccgagcg aggaggagcg gcacuccggg aacaacaucc agcucgacaa gaaccgcauc     4260

uacaagaugg ugagcuccac cagcaagcag ugcuucuuca uccaccaccg ggucgccauc     4320

ucccuggugg acaagaagga guucagcgcc cagaacaaga uggagcgcuc caucgacaac     4380

gagcagguca ugaucaagag cgugugcugg aagcugauca ccgaccggcu cggcaacauc     4440

aucaagguga ucaaguga                                                   4458


<210>  2245
<211>  4461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2245
augaccaaga uccugggccu cgaccugggg accaacagca ucggcugggc cauccgcgag       60

gacgagaacg agggggugaa ccagaucauc gacaagggcg uccggaucuu cuccgagggg      120

gucaagagcg agaacggcaa ggagaucucc cgcgccgcgg agcggacggc cuaccgcagc      180

gcccggaaga ucaaguaccg ccggaagcug cgcaaguacg agacccucaa ggugcugucc      240

gugaacggca ugugcccccu gagcguggag gagguggagc aguggaagaa guccggguuc      300

aaggaguacc cgcucaaccc cgaguuccug aacuggcugc ggaccaacga ggacaagaac      360

aucaaccccu acuucuuccg cgacaaggcc agcaagcaga aggucacccu cuucgagcug      420

ggccgggccc uguaccacau cgcgcagcgc cggggguucc ucuccaaccg ccuggaccag      480

agcgccgagg gcguguucga ggagcacaac ccccagaucc agaaccugau cgaggaccuc      540

gacuccagca acacgauccu gaacgagcug aaggaguacu acaucaaccu cgggaucauc      600

gacgaguccg agaaguccgg cuucaagaag gaccuggacg agggcgagaa gaaccugaag      660

aagcucuaca ccucccuggu gagcaucacc aagaagaacg ccaacgacau cgagaccugc      720

aagcaggagc ugaucgcccg gcucaacaag aaggaggacc uggggaaggu caagggcgag      780

aucaaggaca ucucccaggc gaugcuggac gggaacuuca agacgcucgg ccaguacuuc      840

uucagccugu acaacaagac ccgcauccgg aaccaguaca ccucccgcga ggagcacuac      900

cuggaggagu ucaucaucau cugccagacc cagggcaucg aggagaucaa cacgaacgag      960

aagcucccgg agaagaaguu caccgggcug gccaaggacc uguaccgggc caucuucuuc     1020

cagcgccccc ucaagagcca gaagggccug aucgggaagu gcagcuucga gaagaacaag     1080

ucccggugcg ccgugagcca ccccgacuac gaggaguucc gcaugugguc cuaccugaac     1140

accaucaaga ucggcaccca gagcgagaag acgcuccgcu uccugacccu ggaggagaag     1200

cucaagcugg ugcccaaguu cuaccggaag aacgacuuca acuucgaggu ccuggccaag     1260

gagcucgugg agaagggggc guccuucggc uacuacaaga gcuccaagaa gaacgaguuc     1320

uucuacuggu ucaacuacaa gccgaccgac agcguguccg ccugcgucgu gagcgccucc     1380

cuggagaacg ccaucggcaa ggacuggaag accaagacgu ucgaguacca gaccagcaac     1440

accaagaagg aggaggugac caaguccguc aacuacaagg accuguggca ccuccugagc     1500

guggccacgu ccgacaccua ccuguacgac uucgcgaucg agaagcucaa gcuggagccc     1560

aagaacgcca aggccuucag caagaccaag cugaagaagg acuucgccuc ccugagccuc     1620

gccgcgaucg ccaagauccu gcccuaccug aagcaggggc uccuguacuc ccacgccgug     1680

uucaucgcca acaucgagaa caucgucgac gccgacaucu ggaaggacaa ggagcagcag     1740

aaguucaucc agagcaagau cguggaccug aucgacaacu accucgugga gaaguccaag     1800

cuggagauca ucaacugccu gcucaagauc uacaacaccg aggacaucga gggccgcaag     1860

gucuacuaca gcaaggaggc ggagagccug uucgaggccg accugcggaa gaagcucgug     1920

cccuucuaca aggccaacac gaucuccgag cagcaggagc aggagaucau cuucaaggac     1980

cuguucccgg uccugaucga gcagcucaag aagcaggagu ucaucaagau cgugcgccug     2040

gacaagaaga ucgaggccuu ccugaagggg gagaacgagg agggccagau cuucugcaac     2100

uaccccgaga aguucaagaa gcucuaccac cccagcgaca ucgagguguu caagaagaag     2160

aucaucaagg acgagugggg gaacgagaag gucgugcugg gcagcccccu gaccaccagc     2220

aucaagaacc cgauggcgau gcgggcccuc caccagcugc gcaaggugcu gaacacccuc     2280

auccuggacg gccagaucga cgagaagacg cggauccaca ucgagauggc ccgcgagcug     2340

aacgacgcca acaagcggaa ggggauccag gacuuccaga aggagcgcga ggagcucuac     2400

aagaucuacg aggccaagau caaggagcug uaccagguca agaccggcaa ggagcugcuc     2460

cacgugaccg acgaggaccu gcagaaguuc gagugggcgc uggagcagac caaggacggg     2520

cggaucauca cgaaggagga gcucaucaag uacaagaucu gggaggagca gaaccacauc     2580

ugcaucuaca ccggcaagac caucuccauc gacagcuucc ucgggggcaa ccccaaguuc     2640

gacaucgagc acaccauccc ccgcuccauc agccaggaca acucccugau gaacaagacc     2700

cugugcgaca aggacuucaa ccggagcgug aagggcaaga agaugcccgu cgagcugucc     2760

aacuacaacg agauccugcc gcgcaucgcc cacuggaaga agcaggccga ggagcucacg     2820

cggcagaucg agaccaucag ccgcuccauc aagagcgccg ccaccaaggu ggcgaaggac     2880

aagaacaucc ggaagcgcca cuaccugacc cugaagcggg acuacaucca ggggaaguac     2940

gaccgcuuca cguggggcga gcccaaggug ggguucaaga acucccagau ccccgacacc     3000

ggcaucauca ccaaguacgc ccaggccuac cucaagagcu acuucaagcg cgucgagagc     3060

gugaagggcg gggccguggc cgaguuccgg aagcuguggg gcauccagga guccuucauc     3120

gacgagaacg gguucaagca cuucaaggag aaggaccgca gcaagcacac ccaccacacg     3180

aucgacgcga ucaccaucgc cugcaugccc aaggacaagu acgaccugcu cgcccacgcc     3240

uggcggcugg aggacgagca gaacaagaag gcggccaagg cccugaucga gcaggccaag     3300

ccguggaaga ccuucaagga ggacaucgag aagaucgagg ccgagauccu cgucucccac     3360

uucacccccg acaacgugaa gaagcaggcg aagaagaucc ugcgcgugcg gggcaagaag     3420

caguacgucg ccgagaucga gcgcaacgcc aacgggaagg ccauccccaa gaaggacgcc     3480

agcggcaaga ucaucuacaa gcugaacgag cggggcaaca agauccccau ccuccagcag     3540

ggggacacga uccgcggcuc ccugcaccag gacagcaucu acggggcgau caagaacccg     3600

cugaacacca acgagauccg guacgugauc cgcaaggacc ucgagucccu gaaggccaac     3660

gacaucgaga acaucgugga cgagaacguc aaggccaagg ugaaggaggc cguggccaac     3720

aagauccugc ucaucagcuc caacccccag cagaagaaca aguucgcgga gggcaagaac     3780

gucuggauga acgaggagaa gaagaucccc aucaacaagg ugcggaucua cgccaacagc     3840

gugaagaacc cgaucgagau caagaagcac uccacccuga gcaaguccaa gcacgagcac     3900

aagcagaccg ucuacgggca gaacgacgag aacuacgcca uggcccugua cgagcucgac     3960

ggcaagcgcg aguucgagcu gaucaacaac uucaaccugg cgaagcuccu gaagcagggc     4020

cagggguacu acccccugca caaggagaag gagaucaagg gcaagaaggu gcucaucccc     4080

aucgagaagc ggaacaaccg cgacgugguc cugaagcggg ggcagcaggu cguguucuac     4140

gacaagacgg uggagaacag caaggacauc uccgagaucg uggacuuccg cggccgggug     4200

uaccugaucg aggggcucac cauccagcgc cagaaggaca agaagaccgg caaggucaac     4260

gaguacggca ucauccagcu gcgguacuuc aaggaggccc gcaagagcga ggagaucucc     4320

aaggacagcu ucaagcccga cggggaguuc aagaucaacg agaacaagcc gacccggaag     4380

augaaccaca accaguucac ggccuucgug gagggcaucg acuucaaggu gcugcccucc     4440

gggaaguucc agaagaucug a                                               4461


<210>  2246
<211>  4464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2246
augaagaacg ugcugggccu cgaccugggg agcaacuccg ucggcugggc ccuggugaag       60

accgaggaca acgggaaccc caucggcagc aucaagaugg ggucccgcau caucccgaug      120

agccaggaca uccucggcuc cuucgagaag ggcgacaccg ugagccagac ggcccagcgg      180

acccuguacc gcgggaagcg gcgccugauc gagcggcacg uccuccgccg ggagcgccug      240

ugccgggugc ugcacaucau gaacuuccuc cccgaccacu ucgacaagcu gcugggcugg      300

gacaagaccg acaacaagac cuacgggaag uucaucgacg acuccgagcc caagcucgcg      360

uggcgccaga acaagaacga gaaggagaag auggaguucg uguucaugga cagcuuccac      420

gagaugcugu ccgacuucgc caagcaccag ccccagcuga ucgccaacgg caagaagguc      480

ccgcucgacu ggacgaucua cuaccugcgg aagaaggccc ugacccagcu caucagcaag      540

gaggagcugg ccuggauccu gcucaacuuc aacaagaagc gcggguacua ccagcugcgg      600

ggcgaggagg aggaggagca gcccaccaag aaggaggagu acaaggugcu gaaggugauc      660

uccgucgacg cggacgaggg ccagaagggg aacggcaucu gguacaacau ccaccucgag      720

gacgggggca ucuacaagcg caagagcgac acgccccugu acgacugggu gggcaagacc      780

cugcagcuca ucguggucac caucuacgag aaggacggga agacccccaa gaucuucgac      840

uccggcaagc agaacccgag cuaccggcug cccaaggagg acgacugggg gcugaucaag      900

aagcgcaccg agucccagau ccagagcucc ggcaagacgg ugggggccuu caucuacgac      960

aacauccuca gcaagcccga cgacaagauc cggggcaacc uggugcgcac caucgagcgg     1020

aaguacuaca agaaggagcu gaucgagauc cuccagcagc aggccaagua ccacgacgag     1080

cugcacaacc agaaccugcu cgaguccugc gccaaggagc uguacgcgca caacgacguc     1140

cgcuacaacg agguggucaa gagcaacaug auccaccucc ugguggacga caucaucuuc     1200

uaccagcggc cccucaagag caagaagucc cugaucgcca acugcaagua cgagagcuac     1260

gaguacgugg acaaggagac cggcgaggug aaggagaugc cgaucaagug caucgccaag     1320

uccaaccccu acuaccagga guuccgccug uggcaguuca uccacaaccu gcgccugagc     1380

gacgucguga cccgggagga cgugacggcc caguaccucu ccacgcccca ggacuacugc     1440

aaccuguuca ccuaccugaa cgaccgcaag gagaucaagc aggacauccu ccugaaggac     1500

uucuucaaga ucaagaaggu ccagaucgac aaggagaagg uguuccccau cgaguggaac     1560

uacaucaagg acgccaccaa gaccuacccg ugcaacgaga cccgguacga gcugcugagc     1620

gcgcucaagc gcgccgggau ggacaagcag uggcuggacg acacccccaa caugcaguac     1680

cggcuguggc accuccugua cuccgucgag gagcaggagg agagcgccaa ggcccugcgc     1740

aagcucuacg acaacgacaa cuucgugucc agcuuccuga agaucaagcc cuucaagaag     1800

ugcuacggcg ccuacuccga gaaggcgauc aagcggcugc ucacccugau gcgcaugggg     1860

aaggccuggg acgagcaggc caucgacgcc aagacgcuga agugcaucca gcagaucauc     1920

accggcaaca ucgacagcaa gcucaaggag aagaucgacg acucccggua cgugcugcac     1980

cacgucagcg acuuccaggg gcugcccacc uaccucgccu ccuacgugau cuacggccgc     2040

cacagcgagg ugaccgacau ccggugcugg gacacgccga aggaccugcu gaaguucauc     2100

ugcggcuucc cccagcacuc ccuccgcaac cccaucgucg agcaggucau ccuggagacc     2160

cugcgggugg uguacgacau cuggaaggcg gagaagcaca ucgacgagau ccacguggag     2220

guggcccgcg agaugaagcu caccgcccag cagcggaacg aggccaaccu gcgcaaccug     2280

aacaacgagg ccaccaaccu ccggaucaag uaccugcugg aggagcucaa gaacgacacg     2340

uucaucaagg acguccgccc ccugagcccg augcaccagg agaagaugcg gaucuacgag     2400

caggcggugc uggggaaccu caacaagcag gacaaggacu acgacgacau caucaagauc     2460

uccaagaagg agcgccccac cagcgccgag cugauccgcu acaagcugug gcucgagcag     2520

aaguacugca gccccuacac cggcaaggcc aucucccuga gcaagcuguu caccacggcc     2580

uaccagaucg agcacgugau cccccagucc cgguacuucg acaacagcuu caacaacaag     2640

gucaucugcg aguccgaggu gaacaaggag aaggacaaga ugcucgggua cgaguucauc     2700

aagggccgcg ggggcgaccg gaucuacugc accgcgcacg gcaaggaggu gaccauccug     2760

aaggaggagg aguacaagca guucgucaac gagcacuacg cccacaacaa gcgcaagcgg     2820

gacaaccugc ucgccgagaa caucccggag agcuucucca gccgccagcu gaacgacacc     2880

cgguacauca ccaagacgau caugucccug cucagcaacg uggugcgcca gcagggggag     2940

gaggaggcca ccgccaagaa ccugcugccc uccagcggcg cggucaccga caagcucaag     3000

aacgacuggg ggcugaagga cguguggaac accaucguga cgccccgguu cgagcgccug     3060

aacaagcucu ccggcaccaa cgacuucggc gagaugaagg aggagaacgg gaaccgguac     3120

uuccagacca acgucccgag cgaguacgag aagggcuuca agaagaagcg caucgaccac     3180

cggcaccacg ccauggacgc ccuggugauc gccugcacca cgcgcaacau ggugaacuac     3240

aucuccaacg ucaacgccaa cagccccaag cagcgggagg accugcgcca gcuccugugc     3300

gacaagaacc ggaucaucaa caagcccugg aacaccuuca cccaggacgc gcugaaggcc     3360

cucaacgaca ucgugguguc cuucaagaac aacguccgca ucaucaaccg ggccaccaac     3420

cgcuaccagc gguacgacaa gaacgggaag aagaugaucu gcagccagaa gggcaacgac     3480

augugggcca uccgcaagag caugcacaag gagacggugu ucgggcgcgu gaaccugauc     3540

cggaaggagg cccugcccau cgcgaaggcc cucgacaaca ucuccgccau cugcaacgcc     3600

cagcugcgcg cguacgucaa cgaccuggug gacaagcacu ucaacaagaa gcagcucgug     3660

gcccacuuca agagccugga cuacaagugg aaccggcagg acgucuccaa gguggaggug     3720

uggaucagcu ccgacgacaa gaccccgaug gucgccgugc gcaagccccu ggacaccagc     3780

uucgacaaga agaagaucgc cuccaucacc gacacgggca uccaggccau ccuccugaac     3840

uaccuggaga gcaagggcgg ggacgcgcuc guggccuucg cccccgaggg cauccaggag     3900

augaacucca acaucgagcu guacaacaac gggaaggccc acaagcccau caagaacguc     3960

cggcugagcg agacccucgg cgccaaguac cagguggggg agcgcggcgc gaagaccaag     4020

aaguacgugg ucgccgacaa gggcaccaac cuguucuucg ccaucuacga ggacgaggag     4080

gggaagcggu acuacuacuc cgugccgcug cgcgaggcca ucgagcggca gaagcagaag     4140

cucagccccg ugcccgagua caacgagaag ggcgucgccc ugaaguucua ccuguccccc     4200

aacgaccucg uguacgugcc gacggaggag gagcgcacca uggggaccug ccacaucgac     4260

aagacccgga ucuacaagau ggucagcucc agcgagaacc gcugcugcuu cgugcccucc     4320

acgaucgcga aggucaucgc cgacaaggug gagcugcuga gccacaacaa gaucgagaag     4380

gccaucaccg gcgaguccau caaggaggug ugcuggaagc ucaaggucga ccggcugggg     4440

aacauccuga acgugaucaa guga                                            4464


<210>  2247
<211>  4464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2247
augaagaacg ugcugggccu cgaccugggg agcaacuccg ucggcugggc ccuggugaag       60

accgaggaca acgggaaccc caucggcagc aucaagaugg ggucccgcau caucccgaug      120

agccaggaca uccucggcuc cuucgagaag ggcgacaccg ugagccagac ggcccagcgg      180

acccuguacc gcgggaagcg gcgccugacc gagcggcacg uccuccgccg ggagcgccug      240

ugccgggugc ugcacaucau gaacuuccuc cccgaccacu ucgacaagcu gcugggcugg      300

gacaagaccg acaacaagac guacgggaag uucaucgacg acuccgagcc caagcucgcg      360

uggcgccaga acaagaacga gaaggagaag auggaguucg uguucaugga cagcuuccac      420

gagaugcugu ccgacuucgc caagcaccag ccccagcuga ucgccaacgg caagaagguc      480

ccgcucgacu ggaccaucua cuaccugcgg aagaaggccc ugacccagcu caucagcaag      540

gaggagcugg ccuggauccu gcucaacuuc aacaagaagc gcggguacua ccagcugcgg      600

ggcgaggagg aggaggagca gcccaccaag aaggaggagu acaaggugcu gaaggugauc      660

uccgucgacg cggacgaggg ccagaagggg aacggcaucu gguacaacau ccaccucgag      720

gacgggggca ucuacaagcg caagagcgac aucccccugu acgacugggu gggcaagacg      780

cugcagcuca ucguggucac caucuacgag aaggacggga agacccccaa gaucuucgac      840

uccggcaagc agaacccgag cuaccggcug cccaaggagg acgacugggg gcugaucaag      900

aagcgcaccg agucccagau ccagagcucc ggcaagacgg ugggggccuu caucuacgac      960

aacauccuca gcaagcccgc cgacaagauc cggggcaacc uggugcgcac caucgagcgg     1020

aaguacuaca agaaggagcu gaucgagauc cuccagcagc aggccaagua ccacgacgag     1080

cugcacaacc agcaccuccu ggaguccugc gcgaaggagc uguacgccca caacgacguc     1140

cgcuacaacg agguggucaa gagcaacaug auccaccucc ugguggacga caucaucuuc     1200

uaccagcggc cccucaagag caagaagucc cugaucgaca acugcaagua cgagagcuac     1260

gaguacgugg acaaggagac cggcgaggug aaggagaugc cgaucaagug caucgccaag     1320

uccaaccccu acuaccagga guuccgccug uggcaguuca uccacaaccu gcgccugagc     1380

gacgucguga cccgggagga cgugacggcc caguaccucu ccacgcccca ggacuacugc     1440

aaccuguuca ccuaccugaa cgaccgcaag gagaucaagc aggacauccu ccugaaggac     1500

uucuuccaga ucaagaaggu ccagaucgac aaggagaagg uguuccccau cgaguggaac     1560

uacaucaagg acgccaccaa gaccuacccg ugcaacgaga ccaaguacga gcugcugagc     1620

gcgcucaagc gggccgggau ggacaagcag uggcuggacg acacccccaa caugcaguac     1680

cgccuguggc accuccugua cuccgucgag gagaaggagg agagcgccaa ggcccugcgg     1740

aagcucuacg acgacgacaa cuucgugucc agcuuccuga agaucaagcc cuucgagaag     1800

ugcuacggcg ccuacuccga gaaggcgauc aagcgccugc ucacccugau gcggaugggg     1860

aaggccuggg acgagcaggc caucgacgcc aagacgcuga agugcaucca gcagaucauc     1920

accggcaaca ucgacagcaa gcucaaggag aagaucgacg acucccgcua cgugcugcac     1980

cacgucagcg acuuccaggg gcugcccacc uaccucgccu ccuacgugau cuacggccgg     2040

cacagcgagg ugaccgacau ccgcugcugg gacacgccga aggaccugcu gaaguucauc     2100

ugcggcuucc cccagcacuc ccuccggaac cccaucgucg agcaggucau ccuggagacc     2160

cugcgcgugg uguacgacau cuggaaggcg gagaagcaca ucgacgagau ccacguggag     2220

guggcccggg agaugaagcu caccgcccag cagcgcaacg aggccaaccu gcggaaccug     2280

aacaacgagg ccaccaaccu ccgcaucaag uaccugcugg aggagcucaa gaacgacacg     2340

uucaucaagg acguccggcc ccugagcccg augcaccagg agaagaugcg caucuacgag     2400

caggcggugc uggggaaccu caacaagcag gacaaggacu acgacgacau caucaagauc     2460

uccaagaagg agcggcccac cagcgccgag cugauccgcu acaagcugug gcucgagcag     2520

aaguacugca gccccuacac cggcaaggcc aucucccuga gcaagcuguu caccacggcc     2580

uaccagaucg agcacgugau cccccagucc cgcuacuucg acaacagcuu caacaacaag     2640

gucaucugcg aguccgaggu gaacaaggag aaggacaaga ugcucgggua cgaguucauc     2700

aagggccgcg ggggcgaccg gaucuacugc accgcgcacg gcaaggaggu gaccauccug     2760

aaggaggagg aguacaagca guucgucaac gagcacuacg cccacaacaa gcggaagcgc     2820

gacaaccugc ucgccgagaa caucccggag agcuucucca gccggcagcu gaacgacacc     2880

cgcuacauca ccaagacgau caugucccug cucagcaacg uggugcggca gcagggggag     2940

gaggaggcca ccgccaagaa ccugcugccc uccagcggcg cggucaccga caagcucaag     3000

aacgacuggg ggcugaagga cguguggaac accaucguga cgccccgcuu cgagcggcug     3060

aacaagcucu ccggcaccaa cgacuucggc gagaugaagg aggagaacgg gaaccgcuac     3120

uuccagacca acgucccgag cgaguacgag aagggcuuca agaagaagcg gaucgaccac     3180

cgccaccacg ccauggacgc ccuggugauc gccugcacca cgcggaacau ggugaacuac     3240

aucuccaacg ucaacgccaa cagccccaag cagcgcgagg accugcggca gcuccugugc     3300

gacaagaacc gcaucaucaa caagcccugg aacaccuuca cccaggacgc gcugaaggcc     3360

cucaacgaca ucgugguguc cuucaagaac aacguccgga ucaucaaccg cgccaccaac     3420

cgguaccagc gcuacgacaa gaacgggaag aagaugaucu gcagccagaa gggcaacgac     3480

augugggcca uccgcaagag caugcacaag gagacggugu ucgggcgggu gaaccugauc     3540

cgcaaggagg cccugcccau cgcgaaggcc cucgacaaca ucuccgccau cugcaacgcc     3600

cagcugcggg cguacgucaa cgaccuggug gacaagcacu ucaacaagaa gcagcucgug     3660

gcccacuuca agagccugga cuacaagugg aaccgccagg acgucuccaa gguggaggug     3720

uggaucagcu ccgacgacaa gaccccgaug gucgccgugc ggaagccccu ggacaccagc     3780

uucgacaaga agaagaucgc cuccaucacc gacacgggca uccaggccau ccuccugaac     3840

uaccuggaga gcaagggcgg ggacgcgcuc guggccuucg cccccgaggg cauccaggag     3900

augaacucca acaucgagcu guacaacaac gggaaggccc acaagcccau caagaacguc     3960

cgccugagcg agacccucgg cgccaaguac cagguggggg agcggggcgc gaagaccaag     4020

aaguacgugg ucgccgacaa gggcaccaac cuguucuucg ccaucuacga ggacgaggag     4080

gggaagcgcu acuacuacuc cgugccgcug cgggaggcca ucgagcgcca gaagcagaag     4140

cucagccccg ugcccgagua caacgagaag ggcgucgccc ugaaguucua ccuguccccc     4200

aacgaccucg uguacgugcc gacggaggag gagcggacca uggggaccug ccacaucgac     4260

aagacccgca ucuacaagau ggucagcucc agcgagaacc ggugcugcuu cgugcccucc     4320

acgaucgcga aggucaucgc cgacaaggug gagcugcuga gccacaacaa gaucgagaag     4380

gccaucaccg gcgaguccau caaggaggug ugcuggaagc ucaaggucga ccgccugggg     4440

aacauccuga acgugaucaa guga                                            4464


<210>  2248
<211>  4464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2248
augaagaaca uccugggccu cgaccugggg agcaacuccg ugggcugggc ccuggucaag       60

guggaggaga acgggaaccc caucggcagc aucaagaugg ggucccgcau caucccgaug      120

agccaggaca uccucggcuc cuucgagaag ggcgacaccg ugagccagac cgcccagcgg      180

acgcuguacc gcgggaagcg gcgccugauc gagcggcacg uccuccgccg ggagcgccug      240

ugccgggugc ugcacaucau gaacuuccuc cccgcgcacu ucgaccgccu gcugggcugg      300

gacaagaccg acaacaagac cuacgggaag uucaucgacg acuccgagcc caagcucgcc      360

uggcggcaga acaagaacga gaugggcaag auggaguucg uguucaugga cagcuuccac      420

gagaugcugu ccgacuucgc caagcaccag ccccagcuga ucgccaacgg gaagaagguc      480

ccgcucgacu ggaccaucua cuaccugcgc aagaaggccc ugacgcagcu caucagcaag      540

gaggagcugg cguggauccu gcucaacuuc aacaagaagc ggggcuacua ccagcugcgc      600

ggcgaggagg aggaggagca gcccaccaag aaggaggagu acaaggugcu gaaggugauc      660

uccgucgacg ccgacgaggg gcagaagggc aacgggaucu gguacaacau ccaccucgag      720

gacggcggca ucuacaagaa gaaguccgac aucccccugu acgacugggu ggggaagacc      780

cugcagcuca ucguggucac cacguacgag aaggacggca agacccccaa gaucuucgac      840

agcgggaagc agaacccgag cuaccggcug cccaaggagg acgacugggg ccuggugaag      900

aagcgcaccg agucccagcu ccagagcucc gggaagaccg ugggcgccuu caucuacgac      960

aacauccuga gcaagcccga cgacaagauc cggggcaacc ugguccgcac gaucgagcgg     1020

aaguacuaca agaaggagcu caucgagauc cugcagcagc aggccaagua ccacgacgag     1080

cugcacaacc agaaccuccu ggaguccugc gcgaaggagc uguacgccca caacgacgug     1140

cacuacaacg aggugaucaa gagcaacaug aucaaccucc uggucaacga caucaucuuc     1200

uaccagcgcc cccucaagag caagaagucc cucaucgcca acugcaagua cgagagcuac     1260

gaguacgugg acaaggagac cggggagauc aaggagaugc cgaucaagug caucgccaag     1320

uccaaccccu acuaccagga guuccggcug uggcaguuca uccacaaccu gcgccucagc     1380

gacgugguca cccgcgagga cgugaccgcc caguaccugu ccacgcccca ggacuacugc     1440

aaccuguuca ccuaccucaa cgaccggaag gagaucaagc aggacauccu gcugaaggac     1500

uucuucaaga ucaagaaggu gcagaucgac aaggagaagg ucuuccccau cgaguggaac     1560

uacaucaagg acgcgaccaa gagcuacccg ugcaacgaga cccgcuacga gcugcucucc     1620

gcccugaagc gggccggcau ggacaagcag uggcuggacg acacgcccaa caugcaguac     1680

cgccucuggc accuccugua cagcguggag gagaaggagg aguccgccaa ggcccugcgg     1740

aagcuguacg acgacgacaa guucgucacc agcuuccuga agaucaagcc cuucaacaag     1800

ugcuacgggg cguacuccga gaaggccauc aagcgccucc ugacccugau gcggaugggc     1860

aaggccugga acgagcaggc caucgacgcc aagacccuca agugcaucca gcagaucauc     1920

acggacaacg uggacagcaa gcugaaggag aagaucgacg acucccgcua cgugcugcac     1980

cacgucagcg acuuccaggg gcuccccacc uaccuggcgu ccuacgugau cuacggccgg     2040

cacagcgagg ugaccgacau ccagugcugg gacuccccgg aggaccugcu caaguucauc     2100

cgcgacuuca agcagcacag ccugcggaac cccaucgucg agcaggucau ccuggagacc     2160

cuccgcgugg uguacgacau cuggaaggcc gagaagcaca ucgacgagau ccacguggag     2220

guggcccggg agaugaagcu gacggcccag cagcgcaacg aggccaaccu gcggaaccuc     2280

aacaacgagg cgaccaaccu gcgcaucaag uaccugcucg aggagcugaa gaacgacacc     2340

uucaucaagg acguccggcc ccuguccccc auccaccagg agaagaugcg caucuacgag     2400

caggccgugc ucggcaaccu gaacaagcag gacaaggacu acgacgacau caucaagauc     2460

agcaagaagg agcacccgac caacgccgag cugauccggu acaagcucug gcuggagcag     2520

cgcuacugca gccccuacac ggggaaggcc aucucccuga gcaagcuguu caccaccgcg     2580

uacgagaucg agcacaucau cccccagucc cgcuacuucg acaacagcuu caacaacaag     2640

gugaucugcg aggccgaggu caacaaggcc aagggcaaca ugcuggggua cgaguucauc     2700

aagaagcagg gcgggcagaa gaucuacugc acccagcugg gcaaggccgu gacgauccuc     2760

aaggaggagg ccuacaagca guucgugaac gagcacuacg cgcacaacaa gcggaagcgc     2820

gacaaccugc uggccgagaa cauccccgag uccuucagcu cccggcagcu caacgacacc     2880

cgcuacauca ccaagaccau caugagccug cuguccaacg ucgugcggca gcagagcgag     2940

gaggaggcca ccgccaagaa ccuccugccg uccagcggcu ccgugacgga ccgccugaag     3000

aacgacuggg ggcucaagga cgucuggaac accaucguga cgccccgguu cgagcgccug     3060

aacaagcuga gcggcaccaa cgacuucggg gagaugaagg aggagaacgg caaccgguac     3120

uuccagacca acgugccccu cgaguacgag aagggcuuca agaagaagcg caucgaccac     3180

cggcaccacg ccauggacgc gcuggucauc gccugcacca cccgcaacau ggugaacuac     3240

aucuccaacg ccaacgccaa cagcccgaag cagcgggagg accugcgcca gcuccugugc     3300

gacaagaacc ggaucaucaa caagcccugg gacaccuuca cgcaggacgc ccugaaggcg     3360

cucaacgaca ucguggucuc cuucaagaac aacgugcgca ucaucaaccg ggccaccaac     3420

cgcuaccagc gguacgacaa gaacgggaag aagaucaucu ucagccagaa gggcgacgac     3480

augugggcca uccgcaagag caugcacaag gagaccgugu ucgggcgggu caaccugauc     3540

cgcaaggagg ugcugcccau cgccaaggcg cucgacaaca ucuccgccau cugcaacgcc     3600

cagcugcgcg ccuacgugaa cgaccugguc gacaagcacu ucaacaagaa gcagcucgug     3660

gcccacuuca agagccugaa cuacaagugg aaccggcagg acguguccaa ggucgaggug     3720

uggaucagcu ccgacaacaa gacccccaug guggcggucc gcaagccgcu ggacacgagc     3780

uucgacaaga agaagaucgc cuccaucacc gacaccggca uccagaccau ccuccugaac     3840

uaccuggaga gcaagggcgg ggacgccucc guggccuucg cccccgaggg cauccaggag     3900

augaacagca acaucgagcu cuacaacaac gggaaggcgc acaagcccau caagaacgug     3960

cggcuguccg agccccuggg cgccaaguac caggucgggg agcgcggcgc caagacgaag     4020

aaguacgugg aggccgacaa ggggaccaac cucuucuucg ccaucuacga ggacgaggag     4080

ggcaagcggu acuacuacag cgugccgcug cgcgaggcga ucgagcggca gaagcagaag     4140

cugucccccg uccccgagua caacgagaag ggcguggccc ucaaguucua ccugagcccc     4200

aacgaccugg uguacguccc gaccgaggag gagcgcacca uggggacgug ccacaucgac     4260

aaggcccgga ucuacaagau gguguccagc accggcugcu ccugcuacuu cgugcccagc     4320

accuucgcca aggucaucgu ggacaagguc gagcuccagu cccugaacaa gauggagaag     4380

gcgaucaccg acgagugcau caaggaggug ugcuggaagc ugaaggugga ccgccucggg     4440

aacaucauca acgucaucaa guga                                            4464


<210>  2249
<211>  4485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2249
augaagaaca uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucgac       60

aacgagagcc accgcaucau caagagcggg ucccggauca uccccaugga cgccgcgacc      120

augggcgacu acgagaaggg gaaccucaag agcgccgacu ggaagcgcac guccuuccgg      180

ggcaugcgcc ggcuguacga gcgcgccaag cugcggcgcg agcggcuccu gcgcgugcug      240

aacauccucg gcuuccugcc ggaggaguuc cggcgccaga ucgacuucga gcaccacccc      300

gggcaguuca ucgacggcaa ggagccccug aucgccuacc accacgacga gaacgggcag      360

cgggucuuca gcuacauguc cgccuucgag gagaugcuca ccgacuuccg cgcgacccag      420

agcgagcugg ugcgggacgg ccgccgggug cccuacgacu ggaccaucua cuaccugcgc      480

cacaaggccc ucacgcagcc ggucaccaag gaggagcugg ccuggaucau ccugaacuuc      540

aacaccaagc ggggguacua ccagcuccgc uccgagagcg aggaggugac cacgaacaag      600

aacgaggagu acgccgagcu gaaggugguc uccguggaga agaccggcga ggacaagaag      660

cgggcgggcu acuacuggua caccaucacc uacgacaacg aggccgagca gaagaucacg      720

agcaccaucc cgccccgcca gaucggggac aagguggagc ugaucgucac caccgcccgc      780

gacaaggccg gcgagaucaa gaaguccgug cggagcccca aggaggacga cuggacgcuc      840

augaagaagc gcaccgagca caacaucgag gaggaggagu gcaccguggg guccuacauc      900

uaccagcaca uccuggccga ccccaccguc aaggugcggg gcaagcuggu gcacacgauc      960

gagcgcaagu ucuacaagaa ggagcucaag cagauccuga acaagcagaa ggaguucauc     1020

cccgagcugc aggacgcgag ccucuacgag gccugcauca aggagcugua ccggaacaac     1080

gaggcccaca uccaguccau cgccaacaag gacuucaccg accuguucgu caacgacauc     1140

aucuucuacc agcgcccccu caagagcaag aaguccacca ucagcaacug ccccuacgag     1200

agcuacuacu acaagaacaa ggagaccggc gagcugaucc ggaagccgau caagugcauc     1260

gccaagucca acccccugua ccaggaguuc cgccucuggc aguucgugca caaccugcgg     1320

aucuacaaga aguccgagga ggugagcggg cgccugcaga cggacgucga cgugaccgac     1380

cgguuccuca ccacccccga cgacuacgcg aagcuguuca gcuggcugaa cgaccgcaag     1440

gagaucaagc agaaggagcu ccugcgguac gaggccuucg gccuguccaa gagcuacggg     1500

gacuaccgcu ggaacuucgu ggaggacaag cccuacccgu gcaacgagac gcuccacgag     1560

aucaaccggc gcaugaaguc cauccugagc gacaagaagc uguccagcga ggccuaccag     1620

cagcucuggc acauccugua cuccaucgac gaccccaucg agauccggaa ggcccugacc     1680

agcuucgccc agaaccagga gaucaacucc gacgacuucg ucaacgcguu cuaccaguac     1740

gugcccgaga agaccaagag cuacggcgcc uacuccgaga aggccaucaa gaagcuccug     1800

ccccugaugc gccgggggag cuacugggcc aucggcaaca ucgacgagaa gacccaccgc     1860

cgccuggaca acaucaucaa cggcguggcc gacgacucca ucagcacgug cacccgggag     1920

aagcucaaca accugaccga caucaccaug ugccagggga ugccgcugug gcaggcgugc     1980

uacgccgucu acgaccgcca cuccgaggcc gccgacacga ccaaguggga gcagcccgac     2040

gacaucgaca ccuaccucag caccgagcug aagcagcaca gccugcggaa ccccaucgug     2100

gagaccguca ucuccgagac gcuccgcgug gugcgggaca ucuggaaggc guacggccgc     2160

cccgaggaga uccacgucga gcuggggcgg aaccugaagc aggacgccaa gagccgcaag     2220

caggacgcca uccggauggc cgagaacgag cagaccaacc uccgcauccg ggcccugcug     2280

caggagcucu ccaacccgga gugcggcgug gagaacgugc gcccccacag ccccucccag     2340

auggagcugc ugaagaucua cgagaacgag guccuccaga gcaccgacga cauccccgag     2400

gagaucucca acaucaucaa ggaccugggg aagaccgaca gcaagaagcg gccgucccug     2460

agcgacauca cgaaguaccg ccucuggcug gagcagaagu accagucccc cuacaccggc     2520

cacaucaucc cccugagcaa gcuguucacc ccggcguacc agaucgagca cgugaucccc     2580

cagucccggu acuucgacga cagcaucucc aacaagguga ucugcgaggc cgaggucaac     2640

aaggagaagg accgcaugcu gggcuacgag uucaucauca acaagggcgg gagcaucgug     2700

aagggguccu ucggccagca guucaagauc uucgacaagg ugcaguacga ggacuucguc     2760

aagcagcggu acgcccacaa caagaccaag augaagaagc ugcucaugga cgacaucccc     2820

gacggguuca uccagcgcca gcugaacgac agccgguaca ugagcaagaa gaucaucucc     2880

auccugagca acaucgugcg cgaggacggc gagaaggagg ccacguccaa gcacaucauc     2940

gugaccagcg gcgccaucac cgaccggcuc aagaaggagu gggggaucaa ggacgucugg     3000

aacgacauca ucgcgccccg cuucaagcgc cugaacaagc ugaccgacuc caacaacuac     3060

ggcauggaga cggagaagga ggggcggcag uacuuccaga ucaacgugcc gaccgagcuc     3120

agcaccggcc ugaucaagaa gcgcaucgac caccggcacc acgccaugga cgccaucauc     3180

aucgccugca ccacgcgcaa ccacgugaac uuccugaaca acuccaccgc cgcgagcgac     3240

cagaaggaca uccggcgcga ccuccagcac acccugugca ccaagacgaa gaccgacucc     3300

aacggcaacu acgucuggcg guucaacaag cccugggaca ccuucaccca ggacacgcgc     3360

caggagcugg aggggaucau cgugagcuuc aagcagaacc uccggaucau caacaagaug     3420

uccaacuacu accagcacua cguggacggc cgcaagacca ucagccggca gaacaagggg     3480

gacggcuggg ccauccgcaa gucccugcac aaggccaccg ucagcgggaa ggugaaccug     3540

aagcggucca agaccguggg cuucaaggag gcccucaacg acuggagcgc gaucaaggac     3600

aagacgucca aggccauccu gcagaagauc aucgcccagu acaagaacua cgaccccaag     3660

accaucuuca aguacuucaa ggaccgcaac uucaaggucg agggcaagga cuucagcaag     3720

cuggaggugu acuacaacac cuccgacaag gagcccauga gcgcccaccg ggugaacauc     3780

gacaccacgu ucgacgagaa ggagauccgc agcaucaccg acaccgggau ccagaagauc     3840

augcucaagc accuccacac cuacgacgac gcccagggca agacgcaccc ggaggcggcc     3900

uucucccccg aggggaucga gaagaugaac cagaacaucc ggaugcugaa cggcgggaag     3960

gaccacaagc ccaucuucaa gguccgcaag gccgagaucc ucggcaugaa guucgccgug     4020

ggcgagcggg ggcacaagga caagaagugg guggaggccg acaagggcac caaccuguuc     4080

uucgcgaucu acgagagcga ggagaacaag cgcuccuaca gcuccauccc ccugaacgag     4140

gccaucgacc gccucaagcg cggggagaac aucgccgacg agcgggacca gaacggcaac     4200

aagcugcugu ucguccucag cccgauggac cugguguacg ugcccgaccc cgacgacgcc     4260

cacaucgacg aggucaaggg gaacaacaac aucuucaaga ugguguccug caccggcacc     4320

gagugcuacu ucgugcccga gaccuacgcc aacgucaucu gcaagggcaa ggagcuggag     4380

agcaagaaca agguggaguc cuacgacggg aucaacauca agaaguccug cuacaagauc     4440

acgguggacc ggcucggcaa gguccgcaag aucuucaagg gguga                     4485


<210>  2250
<211>  4491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2250
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc caaggugugc       60

gucgacgaca acgggaacua ccugcgcgac aucaagcucg gcucccggau cauccccaug      120

agccaggacg ugcuggggaa cuucgacaag ggcgugacgg agucccagac cagcguccgc      180

accggcuucc gcggggugcg gcggauccgc gagcgguccc ugcuccgccg ggagcgccug      240

caccgggugc ugcacguccu cggcuaccug ccgccccacu acgacagcgc caucgggugg      300

aacaagaacg agcccuccac cuacggcaag uuccugaacc acggggagcc caagcucgcg      360

ugggagcaca aggaggacgg cagcaugcgc uuccuguuca uggaguccuu ccacgagaug      420

auggccgacu ucgccaagag ccagccgucc cuggugagcg acggcaagaa gaucccccuc      480

gacuggacga ucuacuaccu gcggaagaag gcccuguccc agcccauccg caaggaggag      540

cucgccuggc ugcugcucaa cuucaaccag aagcgggggu acuaccagcu gcgcggcgag      600

gaggacgacg acaaccccac caagugcgag gaguacuacg agcugaaggu ggucgggauc      660

gaggcggacg aggagggcaa gaacggcaac accugguaca acgugaagcu cgagaacggg      720

uggaucuacc ggcgccagag caagaucccg cuggacgacu ggaucggcaa ggugcgggag      780

uucaucguca ccacggagua cgagaaggac gggaucaccc ugaagaagga caagaacggc      840

gacgugaagc gcuccuuccg gagccccucc gaggacgacu gggggcuccg caagaagcgc      900

accgagaagc ugcuggacag cuccaagaug accgugggcg ccuucgucua cgaccaccuc      960

cuggccgagc ccagcgacaa gauccggggc aaguucaucc gcacgaucga gcggaaguac     1020

uacaagaagg agcuguacgc cauccucugc gagcagagca aguaccacga ggagcugcgc     1080

uccggggacu accugaacaa gugcgcggag gagcucuaca agaacaacca gccccaccgg     1140

aacagccugc ugaccaagga ccucgccuac cugcugcucg aggaccugau cuucuaccag     1200

cgcccgcuga agaccaagaa gucccucauc gccgagugcc ccuacgaggc cuacaaguac     1260

guggacaagg agaccggcga gaucaagcgg caggggauca agugcaucgc caagagcaac     1320

cccuacuacc aggaguuccg ccuguggcag uucaucucca accugcgccu cuucaacaag     1380

gcggacgaca aggaggugac ggccgacuac cugcccaccc aggacgacua cguccggcug     1440

uucgccuacc ugaacgaccg gaaggagauc aaccaggaga cccuccugaa ggaguucuuc     1500

aagcugaaga agaucaccgu gaacagcgag aagguguucc cgauccgcug gaacuacauc     1560

gaggacaagg agaagaagua ccccugcaac gagacgcggc acgagcuccu gcuggcccuc     1620

gaccgcgccc ggaucgacca cuccuggcug gagaagcccg gccaggagua ccgccugugg     1680

caccuccugu acagcgucga guccaaggag gagaccaagg aggcgcugcg gaagcucaag     1740

gacgacgacg ccuucgugga gagcuuccug aaggugaagc ccuucaagcg cgaguacggg     1800

gccuacuccg agaaggccau caagaagcug cucgccguca ugcggauggg cagccugugg     1860

cgcgagaccg acaucugcga caccacgaag ggcaacauca ccaagauccu gcagggggag     1920

aucgacgaga agcucaagga gaagaucucc gcgagcgugu ccagcuacaa ccagcugucc     1980

gacuuccagg gccugccggu cuggcucgcc ugcuacgugg uguacgggcg ccacagcgag     2040

gccgccgaga uccagcacug gaagaccccc gagucccugc uggccuacau caacggcuuc     2100

aagcagcaca gccuccggaa ccccaucguc gagcagugca uccuggagac ccugcgcacg     2160

gugcacgaca ucuggaagga gguggggcac aucgacgaga uccacgucga gcucggccgg     2220

aacaugaagu ccaccgcgga ccagcgcgcc cggaucagcg agaacgugcu gcgcaacgag     2280

aacaccaacc ugcggaucaa gagccuccug cuggagcuca agaacgaccc caacaucaag     2340

gacgugcgcc cguacucccc caugcagcag gagaagcugc ggaucuacga ggagggcguc     2400

cugcaggagc ucaccaagga cgacaaggac uucacggaga ucaccaagau cagcaccaug     2460

gcccagcccu ccgccaagga gcugcagcgc uacaagcugu ggcucgagca gaaguaccgg     2520

agccccuaca ccgggaaguc caucagccug acgaagcugu ucaccuccgc guaccagauc     2580

gagcacguga ucccgcagag ccgcuacuuc gacgacuccu ucagcaacaa ggugaucugc     2640

gaguccgagg ucaacagccu caaggacaac augcugggcu acgaguucau caagaaccac     2700

gggggcgaga ucauccacug caccaugcug ggcgacguga aggugcucuc cgagagcgag     2760

uacaaggccu ucgucaacga ccacuacgcc cacaacaagc ggaagcgcga gaaccugcug     2820

cucgaggaca ucccccagga guuccugaac cggcagauga acgacucccg cuacaucagc     2880

aaggugguga aggcccugcu cagcaacauc guccgggccg agggggagga ggaggcgacc     2940

uccaaguucg uggugagcug cuccggcggg aucaccgacc gccugaagaa ggacuggggc     3000

cugaacgacg ucuggaacaa gaucguguac ccccgguucg agcgccucaa ccggcugacg     3060

gggaccgagg ccuucggcca gugggagaac aaggacggca agcgcguguu ccagaccgcc     3120

gucccgcugg agcuccagcg cggguucaac aagaagcgga ucgaccaccg ccaccacgcc     3180

auggacgccc uggugaucgc gugcaccagc cggaacauca ucaacuaccu gaacaacgag     3240

uccgccaaca accccaagaa gcgcgaggac cuccggcaga agcugugcga caagaaccgc     3300

acgauccgga agcccuggga gaccuucacc caggacgcca ccgccgcccu ggaggacgug     3360

aucgucagcu ucaagaacua cgugcgcauc aucaacaagg cgacgaacua cuacgagcgg     3420

uacgacgugg agggcaagaa ggucaucgcc gagcagcgcg gggacgacau gugggccauc     3480

cggaagccca ugcacgaggc caccuacuac ggccgcguga accuccggcg caagaagaag     3540

gugaaccugc gggucgcgcu ggagaacauc uccagcaucu gcgacaacaa gcuccgcauc     3600

uacaucaacg ggcuggugga gaagggcuuc aacaccaagc agauccugga caaguucaag     3660

aacaccaacg accacuucuc caugcagaag aucgacaagg uggagaucug gcaguucacg     3720

aacgaggagg agaagcucgu cgccacccgg aagccgcugg acaccagcuu cgacaccaag     3780

aagaucgccu ccaucacgga caccggcauc cagaccaucc ugcucaacua ccuggagacc     3840

aagggcgggg acagcacgau cgccuucacc cccgagggga ucguggagau gaaccagaac     3900

aucuccaagu acaacaacgg caaggagcac augcccaucc ugaaggugcg ccucuacacc     3960

aagcugggga agaagauccc cgucggccag accggcugca aggccaagca guucgugauc     4020

gcggaggccg ggacgaaccu guacuucgcc aucuacgagg acgaggaggg caagcggagc     4080

uaccgcacca ucccgcucaa cgaggugguc gagcgccuga agcaggggcu gucccccgug     4140

cccgagaaga acgacaagga cgugcaccuc aaguucuacc ugagccccaa cgaccugguc     4200

uacgugccga ucgaggacga gcacgagucc cucgccaagg accacaucua caaguucgug     4260

gacagcuccg gcaccaccgc caacuucauc ccccaccgga gcgcgaacgu caucuucgag     4320

cugaacaaga aggacgccga gaaguucugc agcgggucca ucauccagaa cgaguacggc     4380

gugggcagcu cccagagcaa gaaccagaag gccaucacgg gggagaugau caaggccgug     4440

ugccgcaagc uggaggucga ccggcucggc aacgugauca aggucaucug a              4491


<210>  2251
<211>  4497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2251
augaagcaca uccugggccu cgaccugggg accaacagca ucggcugggc cgugauccgc       60

gcccaccagg acgcggacgg gcacaccacg cugcaggaca uccucgccgc cggcucccgg      120

aucaucccca uggacgccgc cacccugacc gacuucgaca aggggaacgc gcgcagcggc      180

accgccgagc ggacguccuu ccgcagcaug cggcgccggc gcgagcggca gcugcuccgc      240

cgggagcgcc ugcaccgggu ccugaugcuc cugggcuucc ugccgcagca cuacgccaac      300

ucccucaccc gcuacgggga guucgccgac aaccgggagu gcaagcugcc cugggugaag      360

gacgagagcg acggcaagua ccacuuccug uuccaggacg cguuccacga gaugugcgag      420

gacuuccgcc agcucgcgcc ccagugggug gccgacggga agaagguccc cuacgacugg      480

acccuguacu accugcggug caaggcccuc ucccgcaaga ucagcaagga ggagcuggcc      540

uggauccugc ucuccuucaa ccagaagcgg ggcuacuacc aggcccgcga cgaggaggac      600

gaggugacca cgaacaagcu ggaggaguac uacgccggga aggugguccg gguggacgac      660

accggcgagc gcaagggcaa gaacguguac uacgacauca cccuggagaa cggguggguc      720

uaccgcuacg ccgcccggau cuacccggac ugggugggcc aggugaagga guucaucguc      780

accacgaccc ucaaccccga cgggaccccc aagaccgaca aggccggcga ggugaagcgc      840

agcuuccgga ugcccaagga cgacgacugg acgcugauca agaagaagaa ggagcgcgac      900

aucgaccggu cccaggagac caucggccag uacaucuacc acgcgcugcu caaggacccg      960

agccagaaga uccgcgggaa gcugaucggc accguggagc ggaaguacua caaggacgag     1020

cugcgccgga uccucgaccg ccagauccag uuccaccccg agcugcggga ccgcgagcug     1080

uacuacgccu gccuccagga gcuguacccc uccaacgucg ccuuccggaa cacccuggug     1140

aaccgcgacu ucacguaccu cuuccuggac gacauccugu ucuaccagcg gccccucaag     1200

agcaagaagu cccagaucag cgacugcccg uacgaggagc acuucuacac cgacaaggag     1260

accggggagc gcaucuccgu gcccgucaag ugcaucgccg ugagccaccc ccuguaccag     1320

gaguaccggc uguggcaguu cauccagaac cuccgcaucu acugccggga ggcccgcauc     1380

gacggcaacc ugaugaucga cugggaggug accaacgagc ugcuccagac ggaggaggac     1440

aagaccgcgc uguucgacug gcugaacgac aaggaguccg ucaagcagcc cgagcucgug     1500

gccuuccugc ucgggggcaa ggccaacaag aagcagauca aggccuaccg cuggaacuac     1560

auggacgacg acaccaaggg cuacccgugc aacgagaccc gggccagccu gcucaaguac     1620

cugcgcgagg cgcagacgga cagcuccuuc cugacccggg agcgcgagga ggcccucugg     1680

cacauccugu acagcaucuc cgacaagggg gagcucaaga gcgcccuggg caaguucgcc     1740

gugaagcacg ggcugaacga gcgguucguc accguguucu ccaaguuccc gcccuucaag     1800

aaggacuacg gcagcuacuc cgcgaaggcc cugaagaagc uccugccccu gaugcgcaug     1860

gggcaccacu ggcaggaggc cagcuucauc cccuccaccc ggcagcgcau ggagaaguuc     1920

aucaacggcg aguacgacga cagcaucgac aagcggacgu acgagaagcu cgagucccug     1980

accgagguca gccaguaccg cggccugccc ggguggcucg ccugcuacgu gguguacgac     2040

cggcacuccg agggccgcga caucgagcgg uggaccagcc ccuccgacau cgacagcuac     2100

cuggcccagu ucaagcagca cucccugcac aaccccaucg ucgagcgcgu ggugcucgag     2160

acccugcgga cgguccgcga caucuggaag caggucgacc acaucgacga gauccacauc     2220

gagcuggggc ggaacaugaa gcucccggcg gacaagcgcc ggcagaugac cgagcaggcc     2280

uggaagaacg agcagaccaa ccugcgcauc aaggcccugc uccuggaguu cgcccggccc     2340

ggcuacgaca ucgagaacgu gcgccccuac agccccuacc agcaggacca gcugcgcauc     2400

uacgaggagg ccgugcucgg ggcgaacgag ugcccggacg acaucuccgc cauccugaag     2460

aaguucaacg gccugaaccc gcccaccagc gccgaggugc uccgguacaa gugcuggcug     2520

gagcagaagu accgcagccc cuacaccggc cggacgaucc cccuguccaa gcuguucacc     2580

agcgccuacg agaucgagca caucaucccc aagcagcgcu acuucgacga cuccuucagc     2640

aacaagguga ucugcgaguc cgaggucaac aagaagaagg gggccaugcu gggcuacgag     2700

uucaucaaga gcuaccacgg ggcguccauc acccugaacc ggggcggcac cgccagcauc     2760

uucuccgugg aggccuacga gaacuucgug aaggagcacu acgcccacaa ccgcgggaag     2820

augaagaagc uccugaugga cgacgucccc gacagcuuca ucucccggca gcugaacgac     2880

agccgcuaca ucuccaagcu caugaagucc auccugagca accuggugcg ggaggagggc     2940

gagaaggagg cgaucagcaa gaaccucauc gugugcaccg ggggcaucac cgaccgccug     3000

aagaaggacu gggggaucca cgacgucugg aacgacauca ucuucccgcg guucaagcgc     3060

cuggacgaga uggacuccag ccacaccuac aucacccuca acacgcaccg gcaccccauc     3120

cccagcaugc cccuggaguu ccagcgcggc uucuccaaga agcggaucga ccaccgccac     3180

cacgccaugg acgccaucgu gaucgccugc gccacccgga acaucgugaa cuaccugaac     3240

aacgagagcg cgcgccacga cgccaagacc ucccggaugg accuccagcg ccugcugugc     3300

accgccaacc gcaagcucau cgacaagccg uggaagacgu ucacccagga cacgugcaag     3360

guccuggagg acaugaucgu gagcuuccag cggacccugc gcaucaucaa caagacgaag     3420

aacuacuacc uccgguucga ggacggcaag aagcgccggg ugcugcagac caagggggac     3480

aacugggcca uccgcaagcc ccugcacaag gagaccuucu acggcgagau caaccuccgg     3540

cgcaucgaga cccugucccu gaacaaggcc cucgagcgga uccccgacau cgucgagcgc     3600

gaccugcgga agaagcugag cgagcuccug ucccuggggu acaacgcgaa gcgcaucaag     3660

ggcuacuucg aggagcacaa ggaggagugg agcgacguga accucuccaa gaucaaggcc     3720

uacuacuuca cgaagcagaa gaacaagcgg uacuucgccg cccgcaccga gcuggacacc     3780

agcuucaaca aggacaagau cgagaacaag aucgccgaca ccgcgaucca gaagauccug     3840

cuccagcacc uggaggccua cgggaacgac guggagcagg ccuucucccc cgagggcauc     3900

gacacgauga accggaacau ccgccagcug aacggcgggc ggagccacca gccgaucuac     3960

aagguccgcg uguacgagga ggccaccaag uucgcggugg gccggcgcgg gaacaagucc     4020

accaaguucg ucgaggccgc caagggcacc aaccucuucu ucgccaucua cgagaucagc     4080

cagguggacg ggaacggcaa ccggcagacg ucccgcaccu ucagcuccau cccccugaac     4140

guggccgucg agcgccugaa gcagggccuc ccgcccgugc ccgaggacga ggaggggaac     4200

gcgcccaagu acgugcugag ccccaacgac cuggucuacc uccccaccga gaaggagcug     4260

aagcggcgcg agaucgccau gccgaucgac aagucccgga ucuacaagau ggugagcugc     4320

accggcaagg acugcuucuu cauccccgcc aacacggccg ugcccaucgu cgaccgcuac     4380

gaguacagcu ccaugaacaa gaucgggcgg gcccugaccg gcgagaugau caaggagauc     4440

ugccuccccc ugaagaucga ccgccugggg cagaucaucg agaucagcac cuucuga        4497


<210>  2252
<211>  4506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2252
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cgugaucaac       60

aaggccgaga acgaggggaa ggacguccug ugcggcaucg aggcggccgg gucccgcauc      120

auccccaugg acgccaccau caugggcgac uucgagaagg gcaacagcgu gucccagacg      180

aaggaccggc gcgagcaccg gagcacccgc cggcucuacg agcgcgggaa gcugcggcgc      240

gagcggcuga accgcgugcu cuccgucaug ggcuuccugc cggaccacua cgugagccag      300

cuggaccggu acgggaaguu ccucuccaac accgagccca agcuggccug ggugcgcaac      360

gccgacugca aguaccacuu ccuguucaug gacagcuucu ccgagaugcu ccaggaguuc      420

aagaccaaca gcccccagua caucuccgag gagagcaagg uccccuacga cuggacgcug      480

uacuaccugc ggaagaaggc gcucacccac aaggugacca aggaggagcu ggccuggauc      540

cugcucaacu ucaaccagaa gcgcggcuac uuccagcugc gggacgaguc cgaggaggag      600

aagaagccga gcaagauccg ccaguacuuc gagcaccaga agguggucga caucaucgac      660

accaacgagg uguacaaggg gcugaaggug cucaucguca uccuggccaa cggcaucucc      720

ggcaagaucu ucaagaagga gauccccgac uggaucgggg uggagaagga caucaucgug      780

aaggucgaca ucgacaagga cggcaaggac aaguucaacg aggacgggag ccuguccugc      840

cgguucaaca uccccacgga ggccgagugg gacaccgagu gggagcucau caagcagaag      900

acccagaacg accuggacgc gagcggcaag accgugggcg aguacaucuu cgacacgcug      960

cuccgcaacc ccgagcagaa gauccggggg aagcuggugc gcaccaucga gcggcgcuac     1020

uacaaggagg agaucaacgc cauccugaag aagcagaagg aguuccaccc ggagcucucc     1080

gaccgcgccc uguacgagaa gugcgucgag gagcuguacc ccaacaaccc cgcccaccgg     1140

aacaugaugc ucauccacga ccugagcuac uuccugguga acgacauccu cuucuaccag     1200

cgcccccuca agagcaagaa gucccugauc gacgagugcc cguacgagag ccaccucuac     1260

aucgacaaga aguccggcga gcggaagccc guggccguca agugcaucgc caagagccac     1320

ccccuguacc aggaguuccg ccuguggcag uucaucucca accuccggau cuaccagaag     1380

accaaggagg uggggaacag ccugaaggug gacgucgacg ugaccaacga guuccugaag     1440

ggcgagaacg acuacgucga gcuguucgac uggcugaaca cgaagaagga gaucaagcag     1500

gaggacuucc ugaaguacaa gcccuucggg uucaccaagg gcaacaucaa caacuaccgc     1560

uggaacuacg uggaggacaa gaucuacccg ugcaacaaga cccacuacac cauccucaag     1620

ggccuggaga aggcggacau cacguucgac gaguucaucu cccacgacag ccuggccgag     1680

gagaaccugu ggcacauccu cuacuccgug agcuccaagg aggagcugaa gcacgcccug     1740

gagagcuucg ccgacaagcg ggggcucucc aucaaguucg ucgacgcguu caucaacacc     1800

cccgccuucg agaaguccua cggcgccuac agcgccaagg ccaucaagaa gcugcugccc     1860

cucaugcgca uggggaagua cuggaaggag gagaacaucu gcgacagcac ccgggagcgc     1920

aucgacaaga ucaucaccgg cgaguacgac gagaacaucc gggaccgcgu gcgggagaag     1980

uccaucaacc ugacggacau cacccuguuc cgcgggcucc cccuguggcu ggcgugcuac     2040

guggucuacg accggcacag cgaggccggc aacaccgagc gcuggaccuc cccgagcgac     2100

aucgaccacu acauccggaa guucaagcag cacagccucc gcaaccccau cguggagcag     2160

aucgugcugg agacgcugcg gaccguccgc gacgugugga ccgagguggg cgggaucgac     2220

gagauccacg ucgagcucgg ccgcgagaug aagaaccccu ccgacaagcg gcgcaagaug     2280

agccugcaga ugcaggagaa cgagaacacc aaccugcgga ucaaggcccu ccuggccgag     2340

uucaucaacc ccgagcuggg gaucgagaac gugcgcccgu acucccccag ccagcaggac     2400

cuccugcggc uguacgagga gggcgccugg aacucccccg agaucgacga gaagcagguc     2460

gacgagaugc ucgcgauccu gaagaaguuc aacgagacgg acagcuccaa gcgccccacc     2520

aagagcgaga ucaagcggua ccgccugugg cucgagcaga aguaccgguc cccguacacc     2580

gggaccccca ucccccugag caagcuguuc acgaccgccu acgagaucga gcacgugauc     2640

ccgcgcuccc gguucuucga cgacagccuc accaacaagg ugaucugcga guccgaggug     2700

aacaagcuga agggcaacau gcugggcuac gaguucauca agagccacua cggggagaag     2760

guccagcucu ccagcggcuc cgaggugcag aucuucagcg ugaacgacua cgagaaguuc     2820

gucaaggacc acuacagcaa gaacaaggcc aagaugaaga agcugcugau ggacgacauc     2880

cccgagaagu ucaccgagcg ccagaugaac gacucccggu acaucagccg ccucaucgug     2940

ucccugcuga gcaacaucgu gcgggagaag ggggagcagg aguccaucag caagaacguc     3000

cucgugugca ccggcggcgu gacggaccgc cugaagaacg acuggggcgu caacgacgug     3060

uggaacaaga ucauccugcc ccgguucaag cgcaugaacg agaucaccga cuccaccgac     3120

uacaccagcg ugaacacguc cgggcacauc auccccgcca ugccgcucca ccuccagaag     3180

ggguucaaca agaagcggau cgaccaccgc caccacgcga uggacgccau cgucaucgcc     3240

ugcgccggcc ggaacaucgu gaacuaccug aacaaccaga gcuccaagag cgacgugucc     3300

cgccacgacc ugcgcaaccu gcugugcgag aaggacaaga gcgaccggaa ggagcucauc     3360

aucaagcccu gggacaccuu cacccaggac gucuacuccg ugcugagcuc caucaucgug     3420

agcaucaagc agaaccugcg cgucaucaac aagaccaaga acaucuacca gcggaaggag     3480

gacgggaagg ugaucaucuc caagcaggag aacaacauca acuggugggc cauccgcaag     3540

ccccuccaca aggagacggu gagcggcgag gucaaccugc ggaaggagac caccaucagc     3600

cugaaggagg cgaucgacaa ccccgcccgc aucaucgaca aggacuucaa gaagaagcuc     3660

cgggagcuga uccugauggg cuacaacgag aagcucauca agaaguacuu cuacgacaac     3720

aacgaguccu ggcaggacgu gaaccugaag aagaucccgg ccuacuacuu caccaaggac     3780

acgaacgacc gcuacuucgc cagccggucc cugcucgacg agaacuucga caagaagaag     3840

aucgagaaca aggugaccga caccgggauc cgcaccaucc ugcugcggca ccucgagguc     3900

gagggcgggg acagcaaggu ggccuucucc cccgagggcc uggagcgcau gaacaagaac     3960

aucgagaucc ugaacggggg caagaagcac aagcccaucu acaaggugcg ggaguacgag     4020

aaggcggaga aguucgccgu cggcaugcgc gggaacaagg ccagcaaguu cguggaggcc     4080

gccaagggca cgaaccucuu cuucgcggug uacgacaacg ggaagaagcg guccuucgcc     4140

accguccccc ugaacgaggu gauccgccug cagaaggagg gccucccgag cgcccccgcc     4200

aacgaggagg ggcugcugcc caaguucgug cucuccccca acgaccuggu cuacgugccg     4260

accgaggacg agcugcggag cggccuccug cccgagaacc ccgcgcccga gcgcaucuac     4320

aagauggucu ccugcaccgg cuaccagugc uucuucaucc acuucagcgu guccggggug     4380

cugugcgaca agaaggaguu cagcucccuc aacaagaugg agcgggccau cagcggcgag     4440

augaucaagg agaucugcau cccgcugaag gucaaccgcc uggggaagau cauguacaac     4500

cucuga                                                                4506


<210>  2253
<211>  4506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2253
augaagacca uccugggccu cgaccugggg accaacagca ucggcugggc caagaucucc       60

aucgacgaca acgggaacua ccugcgcgac auccagcucg gcagccggau cauccccaug      120

ucccaggacg ugcuggggaa guucgacaac ggcgucacgg agagccagac cgccgugcgc      180

accggcuucc gggggcugcg ccggauccgc gagcgguccc uccugcgccg ggagcgccug      240

caccgggugc uccacgucau gggcuuccug ccgccccacu acgacagcgc gaucgggugg      300

aacaaggagg agcccuccac cuacggcaag uuccugaacc acggggagcc caagcucgcc      360

ugggagcgcc agggcgacgg cagcaugcgg uuccuguuca uggacuccuu ccacaagaug      420

auggaggacu ucgccccgca ccagcccgcc cugguggccg acgggcgcaa gaucccccuc      480

gacuggacgc uguacuaccu gcggaagaag gcgcucaccc agcccaucaa gaaggaggag      540

cuggccugga uccugcucaa cuucaaccag aagcgcggcu acuaccagcu gcgcggggag      600

gaggaggagg agaacccgac caagcgggag gaguaccacg agcugaaggu ggucagcgug      660

gaggccgccg aggagggcaa gggcgggaac accugguaca acgugaagcu cgagaacggc      720

uggaucuacc accggcccuc caagaucgac cugagcgacu gggucgggaa gacgaagcag      780

uucaucguga ccauggagua cgaggcggac ggcaucaccc ugaagaagaa caaggagggg      840

gaggugaagc gcuccuuccg gguccccgac gaggacgacu ggggccuccg caagaagcgc      900

accgagagcc agcuggaggc cuccggcaag acgguggggg ccuucaucua cgaccaccuc      960

cuggccgagc ccgccgacaa gauccggggc aacuucaucc gcaccaucga gcggaaguac     1020

uacaagcagg agcuggcgga cauccugcgc gagcagagca aguaccacga ggagcuccag     1080

uccaagaaga uccuggccga cugcgccgag gagcuguacc ggagcaacca gccgcaccgc     1140

gacucccucc ugaagaagga caugguguac cugcuccuga ccgaccugau cuucuaccag     1200

cggccccuca agagcaagaa gucccugauc gccaacugcc ccuacgagua cuacgaguac     1260

gucgacaagg agaccgggga gaucaagcgc cagggcauca agugcguggc caagagcaac     1320

cccuacuacc aggaguuccg gcuguggcag uucgugcuca accugcgccu guacgaccgg     1380

acggaggaca aggaggucac cgcggaguac cucccgagca aggaggacua cgcccgccug     1440

uucguguacc ugaacgaccg gaaggagauc aaccaggaga cccugcucaa ggacuucuuc     1500

aagcugaaga aggugaagca gcgcuccgag aaggaguucc ccguccggug gaacuacauc     1560

gaggacaagg agaagaagua ccccugcaac gagacccgcc acgagcugcu cauggcccug     1620

gaccgggccg ggauggagcg cagcuggcug gacgacacgg cccggcagua ccgccucugg     1680

caccuccugu acuccaucga ggugaaggac gaggcggagc gcgcccugcg gaagcugaag     1740

gacgacgacg aguucgugag cgccuuccug aaggucaagc ccuucaagaa ggacuacggc     1800

gccuacuccg agaaggccgu gaagaagcuc cuggcgguca ugcgcauggg cagccacugg     1860

ugcgaggacg acaucugcaa ggagaccaag gagcggaucg agcacauccu gcagggggac     1920

guggaggaga agaucaagga gcgcaugaac cacaccgccu acucccucgg cgagcugagc     1980

gacuuccagg ggcugccgga guggcucgcc ugcuacgugg ucuacggccg gcacuccgag     2040

gccaccgaca ucaagcacug gaagacgccc gagagccuga uggcguacau caacggguuc     2100

aagcagcacu cccugcgcaa ccccaucgug gagcagugca uccucgagac ccugcggacc     2160

gugcacgaca ucuggaagga ggccggccac aucgacgaga uccacgucga gcugggccgc     2220

agcaugaagu ccaccagcga ccagcgggcc cgcaugacgg agaacauccu ccggaacgag     2280

aacaccaacc ugcgcaucaa gucccugcuc auggagcuga agaacgacgc cgacaucaag     2340

gacgugcggc ccuacagccc gaugcagcag gagauccugc gcaucuacga ggagggggcc     2400

cuccaggagc ugaccaagga ggacaacgac uacgaggaga ucuucaagau cuccaagauc     2460

ccccagccca gcgcgaagga gcugcucaag uacaagcugu ggcuggagca gaaguaccgg     2520

agccccuaca ccggcaagcc gaucucccuc agcaagcugu ucacguccgc cuaccagauc     2580

gagcacauca ucccccagag ccgcuacuuc gacgacuccu ucaacaacaa ggugaucugc     2640

gagagcgagg ucaacgcccu gaaguccaac augcucgggu acgaguucau caagaaccac     2700

ggcggcgaga ucgugcacug cacccugcug ggggacguga agguccagaa cgagagcgag     2760

uacaaggccu ucgugaccga gcacuacgcc aacaacaagu acaagaagga gaagcuccug     2820

auggaggaca ucccccagga cuuccugaac cggcaccuca acgacucccg cuacaucagc     2880

aaggugguca agacccugcu guccaacauc gugcggacgg aggacgagau ggagaccacc     2940

agcaaguucg uggucuccug caccggcggg aucaccgacc gccucaagaa ggacuggggc     3000

cugaacgacg uguggaacag caucguguac ucccgguucg agcgccugaa cgagcucacc     3060

aagagcgacg cguucgggcg cugggagaac aaggagggca agcgggucuu ccagacgucc     3120

gugccgcugg agcugcagcg cggcuucaac aagaagcgga ucgaccaccg ccaccacgcc     3180

auggacgccc ucgugaucgc cugcgccagc cggaacauca ucaacuaccu gaacaacgag     3240

agcgcgaacu cccccaagaa gcgcgaggac cugcggcagc agcucugcga caagaaccgc     3300

aucauccgga agcccuggga gagcuucacc caggacgccc gcgccgcccu ggaggacguc     3360

gugguguccu ucaagaacua cguccgggug aucaacaaga ccacgaacua cuacgagcgc     3420

uacggggcgg acggcgagaa gaccaucgcc aagcagaaga acagcgagau gugggccgug     3480

cggaagccca ugcacgaggc caaguacuac gggcgcguca accugcagcg gaagaccgag     3540

gugcgccuca aggccgcgcu ggaggacauc ccguccaucu gcaacaagga gcugcgggac     3600

uacaucaacc gccucgucaa gagcggccug aacgcccggc agcugcucgc ccacuucaag     3660

uccaccgacu acugcuggaa caagcagaac guggacaagg uggaggugug gcgcuucagc     3720

gacgagaagg agccccuggu cgccauccgc gagucccugg acacgagcuu cgacgccaag     3780

cggaucgaca ccaucaccga caccgggauc cagaagaucc uccugaacua ccuggaggcg     3840

aagggcggcg accccgccau cgccuucacg cccgagggga ucgccgagau gaaccagaac     3900

auggcccucu acaacaacgg caagaugcac cagccgaucc ugcgcgugcg gcuguccaag     3960

aagcgcggga agaaguacca ggugggccag accgggugca aggcgaagca guucgucauc     4020

agcgaguccg gcaccaaccu cuacuucgcc aucuacgagg acgaggaccg gaaccgcacc     4080

uacaacacga ucccccugaa cgagguggcc gagcggcuga agcagggccu cagccccgug     4140

cccgagaaga acgagaagga ggucccgcug aaguucuacc ugucccccaa cgaccucgug     4200

uacgugccca ccgaggagga gauccugaug aaggagugca agcugaacaa gaaccgcauc     4260

uacaaguuca ucgacagcuc cgggaccacc gccaacuucg ucccccaccg gagcgcgucc     4320

cucaucuaca acguggacaa gaagcuggcc gaggccuucu gcaacgacga gaucauccag     4380

aacgaguacg gcguggggag cccgcagagc aagaaccaga aggcccugac gggcgagaug     4440

aucaaggccg ucugcuggaa gcucgaggug gaccgccugg ggaacaucau ccgggugauc     4500

aaguga                                                                4506


<210>  2254
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2254
augaagaaca ucgugggccu ggaccucggg accaacagca ucggcugggc cgucgugaac       60

gggaacauca acgacgacgg cuccgagcag cuggugaaga uccaggccag cgggucccgc      120

aucaucccca uggacgcggc caugaucggc gacuucaaca agggcaacag caucucccag      180

accgccgagc ggacgcgccu gcgggggauc cgccggcuca gcgagcgcua ccugcugcgg      240

cgcgagcggc uccaccgcau ccuggacauc cugggcuucc ucccguccca cuucgcccgg      300

gaccuggacc gcuacgggaa gaucgucaag gacaaggagc ccaagcuggc cuggcggaag      360

aacgaggcgg gccaguucga guucaucuuc cagaacagcu ucaaggagau gcucgaggac      420

uucaagcuga accaccccaa ccugaucacc gacgacaaga aggugcccua cgacuggacc      480

aucuacuacc uccgcaagaa ggggcugacc uccaagauca gcaaggagga gcuggccugg      540

auccuccuga acuucaacca gaagcggggc uacuaccagc ugcgcggcga ggaggaggag      600

gagaacaaga acaagcaggu cgaguucuac gcccucaagg ugaucgccgu ggaggacucc      660

ggggagaaga agggcaagga cacgugguac aacgugcacc uggagaacgg guggaucuac      720

cggcgcacca gcaacaugcc gcuggacugg gucggcaaga ccaaggaguu caucgugacc      780

acggaccucg agaaggacgg cacccccaag aaggacaagg aggggaacau caagcggucc      840

uuccgcgcgc ccaaggagga cgacuggagc cuggugaaga agaagaccga ggccgacauc      900

gaccagaccc acaagacggu cggcaccuac aucuacgaca cccugcucgg gaaccccucc      960

cagaagaucc ggggccgccu ggugcgcacc aucgagcgga aguacuacaa ggaggagcug     1020

aagcucaucc uggacaagca gaaggaguuc cacccggagc ugcaggaccg cgcccucuac     1080

gccgccugcg uggaggagcu guacacgauc aacgaggcgc accggaacaa caucgggaac     1140

cgcgacuuca ccuaccuguu ccucgaggac auccuguucu accagcggcc ccucaagagc     1200

aagaaguccc ugaucgacaa cugccccuac gaggagaacc acugccugga caaggagacc     1260

ggcgagauca aguucuuccc cgucaagugc aucgccaaga gccacccgcu guuccaggag     1320

uuccgccucu ggcaguucau cgugaaccug cggaucuacc gcaagggccu ggcccaggac     1380

guggacguca ccaacgagcu ccugaagacg gaggaggacu acguggcccu guucgacugg     1440

cucaacggga agaaggagau cgaccagaag gccuuccuga aguacccgcc cuucggccug     1500

aagaaggagg uggagaacua ccgguggaac uacguccagg acaaguccua ccccugcaac     1560

gagacccgca gccugaugcu cgcgcggcug gagaagugcg acaucagcau cgaguuccug     1620

accaaggaga acgaggaggc ccucuggcac auccuguacu ccguggagga caagaacgag     1680

aucgugaagg cccugcagaa guucgccgac aagcacaacc ucaacgccag cuucgucgac     1740

guguuccaga aguuccccuc cuucaagaag gaguacggga gcuacuccgc gaaggccauc     1800

aagaagcugc ugccccucau gcgcaugggc aaguacugga gcgaggacuc caucgacaag     1860

gggacccggg agcgcaugga caagaucauc acgggcgagu acgacgagaa gaucgagaac     1920

cggguccgcg agaaggccau ccaccuggcc gacaucagcg aguucaaggg ccugccccuc     1980

uggcuggugu gcuacaucgu guacgggcgg cacuccgaga gcaaggagau cgccaagugg     2040

gagucccccg cggacaucga cagcuaccug aaguccuuca agcagcacag ccuccgcaac     2100

ccgaucgucg agcagaucau caucgagacc cugcgggugg ugcgcgacau cuggaagcag     2160

gucggcaaca ucgacgagau ccacguggag cuggggcgcg agaugaagaa ccccgccgac     2220

aagcggcgca agaugaccca gcagaucucc gagaacgaga acaccaaccu ccggaucaag     2280

uaccugcuga cggaguuccu caaccccgag uacgaggugg agaacguccg ccccuacagc     2340

ccgucccagc aggacauccu gcggaucuac gaggagggcg ugcugaacag cguguccaac     2400

cuccccgagg agaucagcga cauccugaag aaguucaccg agaccgaccu gaagaagcgc     2460

cccagccggu ccgagguccu ccgcuacaag cuguggcugg agcagaagua ccggagcccc     2520

uacaccgggg aggugauccc gcucggcaag cuguucacgc ccgccuacga gaucgagcac     2580

gugauccccc agucccgcua cuucgacgac agcuucucca acaaggucau cugcgaggcc     2640

gaggugaaca agcucaagag caaccugcug ggccacgagu ucaucgugaa gcaccacggg     2700

gagaucgucg agcugccguu cggcaagaag gugaagaucu ucaccgugga cgaguacgag     2760

caguucguca aggacaacua cucccggacc cgcgggaaga ugaagaagcu ccugguggac     2820

gacauccccg aggaguucgu ggcgcggcag cugaacgaca gccgcuacau cuccaagguc     2880

gucaagagcc uccuguccaa caucgugcgg gagaagggcg aggaggaggc caucagcaag     2940

aacgugaucc ccugcaccgg cggggugacg gaccgccuga agaaggacug gggcgugaac     3000

gacgucugga acaagaucau ccucccccgg uuccagcgcc ugaacgagcu gaccgggacc     3060

aacaaguuca ccaccauguc ccucaacaac cacgagaucc cggccaugcc ccuggagcug     3120

cagaagggcu ucaacaagaa gcggaucgac caccgccacc acgccaugga cgccaucauc     3180

aucgcgugcg ccaaccggaa caucaucaac uaccucaaca acgagagcgc cacggccaag     3240

gccgagcugu cccgcuacga ccugcagcgc cuccugugcg acaaggcgaa gaccgacgac     3300

aacgggaacu acaagugggu gauccggaag cccugggcca gcuucaccca ggacgugcac     3360

ggcguccugg aggacaucau cguguccuuc aagcagaacc uccgcgugau caacaaggcc     3420

accaaccacu uccugcacua caacgaggag ggcaagaaga ucuucgucaa gcaggagaag     3480

ggggacagcu gggccauccg caagagcaug cacaaggaca cgguguucgg cgaggugaac     3540

cugcggcgga ucaagaccgu cgcccucaac gaggcgauga agaaccccca guccaucgug     3600

gugaaggacu ucaagcgcaa gcugcuggag cuccugaacc ugggguucga cgccaagcgg     3660

aucaagaagu acuucgagga caaccgcgag accuggagcg acaucaaccu cuccaagauc     3720

accgucuacu acuucagcaa ggacacgaag gaccgguucu ucgccacccg caagccgcug     3780

gacaccuccu ucgaccggaa gaagaucgag aacaacauca ccgacacggg cauccagaag     3840

auccugcucc gccaccugga gcugaaggac aacaaccccg acaucgccuu cagccccgac     3900

gggaucgacg agaugaaccg gaacaucauc cagcucaaca acggcaagua ccaccagccc     3960

aucaucaagg ugcgcuggua cgagcaggcg gacaaguucg ccgugggcca gaccgggaac     4020

aaguccagca aguucgucga ggccgccaag ggcaccaacc uguucuucgc cguguacgag     4080

uccaacaucc uggacgagaa gaccaacacg aucaucaaga agcggaacua cgcgaccauc     4140

ccgcucaacg uggccaucga gcgccagaag caggggcuga gcgucgcccc cgaggaggag     4200

aacggcaacg accccaucuu cgugcugucc cccaacgacc ucguguaccu gccgaccacc     4260

gacgagcugg ccaacggggu cguggcccag ccccucgaca aggagcggau cuacaagaug     4320

gucagcugca cgggcaacga gggccacuuc auccccgcgc gcaucgccaa ccccauccug     4380

cagaccaucg agcugggguc caacaacaag gcccagaagg ccuggaccga cgagaugauc     4440

aaggagaucu gcaugccgau caagguggac cggcucggca acauccugga gagcuccagc     4500

cugcacaaga aguga                                                      4515


<210>  2255
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2255
augaagaaca ucgugggccu ggaccucggg accaacagca ucggcugggc cgucgugaac       60

ggguccguga acgacgacgg cagcgagcag cuggucaaga uccaggccuc cgggagccgc      120

aucaucccca uggacgcggc caugaucggc gacuucaaca agggcaacuc caucagccag      180

accgccgagc ggacgcgccu gcgcggggug cggcggcucu ccgagcgcua ccugcugcgg      240

cgcgagcggc uccaccgcau ccuggacauc cugggcuucc ucccguucca cuucgcccag      300

gaccuggacc ggcacgggaa gaucgugaag ggcaaggagc ccaagcuggc cuggcgcaag      360

aacgaggcgg ggcaguucga guucaucuuc caggacagcu ucaaggagau gcucgaggac      420

uucaagcuga acuaccccaa ccugaucacc gacgacaaga agguccccua cgacuggacc      480

aucuacuacc uccggaagaa gggccugacc uccaagauca gcaaggagga gcuggccugg      540

auccuccuga acuucaacca gaagcgcggc uacuaccagc ugcgcgggga ggaggaggag      600

gagaacaagg acaagcucgu ggaguucuac gcccugaagg uggucgccgu ggaggacucc      660

ggcgagaaga aggggaagga caucugguac aacgugcacc uggagaacgg cugggucuac      720

cggcggacga gcaacauguc ccucgacugg gugggcaaga ccaaggaguu caucgugacc      780

accgaccugg agaaggacgg gacgccgaag aaggacaagg agggcaacgu caagcgcagc      840

uuccgggcgc ccaaggagga cgacuggucc cuggugaaga agaagaccga ggccgacauc      900

gaccagaccc acaagaccgu gggggccuac aucuacgaca cgcuccuggg caaccccagc      960

cagaagaucc gcgggcggcu gguccgcacc aucgagcgca aguacuacaa ggaggagcuc     1020

aagcugaucc uggagaagca gaaggaguuc caccucgagc ugcaggaccg ggcccuguac     1080

gccgcgugca ucgaggagcu cuaccccauc aacgaggccc accgcaacaa cgugggcaac     1140

cgggacuuca ccuaccuguu ccuggaggac auccucuucu accagcgccc gcugaagucc     1200

aagaaguccc ugaucgacaa cugccccuac gaggagaacc agggccucga caaggagacc     1260

ggggagauca agagcuuccc cgugaagugc aucgccaaga gccacccccu guuccaggag     1320

uuccggcugu ggcaguucau caucaaccuc cgcaucuacc ggaaggaccu ggcccaggac     1380

gucgacguga cgaacgagcu gcucaagacc gaggaggacu acguggcccu guucgacugg     1440

cugaacggca agaaggagau cgaccagaag gcguuccuga aguacccggu cuucgggcuc     1500

aagaaggaga ucgagaacua ccgcuggaac uacgugcagg acaagcccua ccccugcaac     1560

gagacccgga gccugaugcu ggcccgccuc gagaagugcg acaucuccau cgaguuccug     1620

accaaggaga acgaggaggc ccuguggcac auccucuaca gcgucgagga caagaacgag     1680

aucgugaagg cccugcagaa guucgccgac aagcacgacc ugaacgcguc cuucguggac     1740

gucuuccaga aguucccgcc cuucaagaag gaguacggca gcuacuccgc caaggccauc     1800

aagaagcucc ugccccugau gcggaugggg aaguacugga gcgagaacuc caucgacaag     1860

ggcacgcgcg agcggaucga caagaucauc accggcgagu acgacgagaa gaucgagaac     1920

cgcgugcggg agaaggccau ccaccucgcc gacgugagcg acuucaaggg gcugccccug     1980

uggcucgucu gcuacaucgu guacggccgc cacuccgagg cgaaggagau cauccagugg     2040

aagucccccg ccgacaucga cagcuaccug cggagcuuca agcagcacuc ccugcgcaac     2100

ccgaucgugg agcagaucau caucgagacc cuccgggucg ugcgcgacau cuggaagcag     2160

gucgggaaca ucgacgagau ccacguggag cugggccgcg agaugaagaa ccccgccgac     2220

aagcggaaga agaugaccca gcagaugagc gagaacgaga acacgaaccu gcgcaucaag     2280

uaccuccuga ccgaguuccu gaaccccgag uacgaggugg agaacgugcg gcccuacucc     2340

ccgagccagc aggacauccu ccgcaucuac gaggaggggg uccugaacuc cgugagcgac     2400

cugcccgagg agaucuccga cauccucaag aaguucaccg agaccgaccu gaagaagcgg     2460

cccagccgca gcgaggugcu gcgguacaag cucuggcugg agcagaagua ccgcuccccc     2520

uacacgggcg aggucauccc gcugggcaag cuguucaccc ccgccuacga gaucgagcac     2580

gugauccccc agagcugcua cuucgacgac uccuucagca acaaggugau cugcgaggcg     2640

gaggucaaca agcucaaguc caaccugcuc gggcacgagu ucaucgugaa gcaccacggc     2700

gagaucgugg agcugccguu cgggaagaag guccggaucu ucaccgugga cgaguacgag     2760

caguucguga aggacaacua cagccgcacc cgguccaaga ugaagaagcu gcucauggac     2820

gacauccccg aggaguucgu cacgcgccag cugaacgaca gccgguacau cuccaaggug     2880

gucaagagcc ugcucuccaa caucgugcgc gagaagggcg aggaggaggc caucagcaag     2940

aacgugaucc ccugcaccgg cggggugacc gaccggcuga agaaggacug gggcaucaac     3000

gaggucugga acaagaucau ccugccccgc uuccagcggc ucaacgagcu gaccgggacg     3060

aacaaguuca ccaccaagaa cgugggcauc caggagaucc cgaccaugcc ccuggagcuc     3120

cagaaggggu ucaacaagaa gcgcaucgac caccggcacc acgccaugga cgccaucauc     3180

aucgccugcg cgaaccgcaa caucaucaac uaccugaaca acgaguccgc caccgccaag     3240

gccgagcuga gccgguacga ccuccagaag augcugugcg acaaggccaa gacggacaac     3300

aacggcaacu acaagugggu gauccgcaag cccugggcgu ccuucaccca ggacaccuac     3360

cuggcccucg agaacaucau cgucagcuuc aagcagaacc ugcgcgugau caacaaggcc     3420

accaaccggu uccugcacua caacgaggag ggcaagaaga ucuucgugaa gcaggggaag     3480

ggcgacaacu gggccauccg caaguccaug cacaaggaca cggucuucgg ggaggugaac     3540

cuccggcgca ucaagaccgu ggcccugaac gaggcgauga agaaccccca gagcaucguc     3600

gugaaggacu ucaagcggaa gcugcucgag cuguggaacc ugggcuucga cgccaagcgc     3660

aucaagaagu acuucgagga caaccgggag accuggagcg acaucaaccu cuccaagauc     3720

gagguguacu acuucagcaa ggacaccaag gaccgcuucu ucgccacgcg gaagccgcug     3780

gacaccuccu ucgaccgcaa gaagaucgag aacaacauca ccgacaccgg gauccagaag     3840

auccugcucc ggcaccugga gcugaaggac aacaaccccg acaucgccuu cagccccgac     3900

ggcaucgacg agaugaaccg caacaucauc cagcucaaca acggcaagua ccaccagccc     3960

aucaucaaga uccgguggua cgagcaggcg gacaaguucg ccgucgggca gacgggcaac     4020

aaguccagca aguucgugga ggccgccaag gggaccaacc uguucuucgc cguguacgag     4080

uccaacaucc uggacaagaa gaccaacacc aucaucaaga agcgcaacua cgcgacgauc     4140

ccgcucaacg ucgccaucga gcggcagaag cagggccugc ccguggcccc cgaggacgag     4200

aacgggaacg accccaucuu cgugcugagc ccgaacgacc ucgucuaccu gcccaccgac     4260

gacgagcugg ccaacggcau caucgcccag ccccucgacc gcggccggau cuacaagaug     4320

guguccagcu ccggggagca gugcuucuuc aucaagcaca ucgucgcgaa cgugcuggug     4380

gacaaguucg aguucagccc ccugaacaag auggagcgcg cccucaccgg cgagaugauc     4440

aagaugaucu gcaucccgau caaggucgac cggcugggga acgugcugga guccagcucc     4500

agcuacaaga aguga                                                      4515


<210>  2256
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2256
augaagaaca ucgugggccu ggaccucggg accaacagca ucggcugggc cgucgugaac       60

ggguccguga acgacgacgg cagcgagcag cuggucaaga uccaggccuc cgggagccgc      120

aucaucccca uggacgcggc caugaucggc gacuucaaca agggcaacuc caucagccag      180

accgccgagc ggacgcgccu gcgcggggug cggcggcucu ccgagcgcua ccugcugcgg      240

cgcgagcggc uccaccgcau ccuggacauc cugggcuucc ucccguucca cuucgcccag      300

gaccuggacc ggcacgggaa gaucgugaag ggcaaggagc ccaagcuggc cuggcgcaag      360

aacgaggcgg ggcaguucga guucaucuuc caggacagcu ucaaggagau gcucgaggac      420

uucaagcuga accaccccaa ccugaucacc gacgacaaga agguccccua cgacuggacc      480

aucuacuacc uccggaagaa gggccugacc uccaagauca gcaaggagga gcuggccugg      540

auccuccuga acuucaacca gaagcgcggc uacuaccagc ugcgcgggga ggaggaggag      600

gagaacaaga acaagcucgu ggaguucuac gcccugaagg uggucgccgu ggaggacucc      660

ggcgagaaga aggggaagga caucugguac aacgugcacc uggagaacgg cugggucuac      720

cggcggacga gcaacaugcc gcucgacugg gugggcaaga ccaaggaguu caucgugacc      780

accgaccugg agaaggacgg gacgcccaag aaggacaagg agggcaacgu caagcgcucc      840

uuccgggcgc ccaaggagga cgacuggagc cuggugaaga agaagaccga ggccgacauc      900

gaccagaccc acaagaccgu gggggccuac aucuacgaca cgcuccuggg caaccccucc      960

cagaagaucc gcgggcggcu gguccgcacc aucgagcgca aguacuacaa ggaggagcuc     1020

aagcagaucc uggagaagca gaaggaguuc caccuggagc uccaggaccg ggcccuguac     1080

gccgcgugca ucgaggagcu guacccgauc aacgaggccc accgcaacaa cgugggcaac     1140

cgggacuuca ccuaccucuu ccuggaggac auccuguucu accagcgccc ccucaagagc     1200

aagaaguccc ugaucgacaa cugccccuac gaggagaacc agggccugga caaggagacc     1260

ggggagauca agagcuuccc cgugaagugc aucgccaagu cccacccccu cuuccaggag     1320

uuccggcugu ggcaguucau caucaaccug cgcaucuacc ggaaggaccu cgcccaggac     1380

gucgacguga cgaacgagcu gcugaagacc gaggaggacu acguggcccu cuucgacugg     1440

cugaacggca agaaggagau cgaccagaag gcguuccuga aguacccggu cuuccgccug     1500

aagaaggaga ucgagaacua ccgguggaac uacgugcagg acaagcccua ccccugcaac     1560

gagacccgca gccucaugcu ggcccggcug gagaagugcg acaucagcau cgaguuccuc     1620

accaaggaga acgaggaggc ccuguggcac auccuguacu ccgucgagga caagaacgag     1680

aucgugaagg cccuccagaa guucgccgac aagcacgacc ugaacgcgag cuucguggac     1740

gucuuccaga aguucccgcc cuucaagaag gaguacgggu ccuacagcgc caaggccauc     1800

aagaagcugc ucccccugau gcgcaugggc aaguacuggu ccgagaacag caucgacaag     1860

gggacgcggg cccgcaucga caagaucauc accggcgagu acgacgagaa gaucgagaac     1920

cgggugcgcg agaaggccau ccaccuggcg gacguguccg acuucaaggg ccucccccug     1980

uggcuggucu gcuacaucgu guacgggcgg cacagcgagg ccaaggagau cauccagugg     2040

aaguccccgg ccgacaucga cagcuaccuc cgcuccuuca agcagcacag ccugcggaac     2100

cccaucgugg agcagaucau caucgagacc cugcgcgucg ugcgcgacau cuggaagcag     2160

gucggcaaca ucgacgagau ccacguggag cucgggcggg agaugaagaa ccccgccgac     2220

aagcgcaaga agaugaccca gcagaugucc gagaacgaga acacgaaccu gcggaucaag     2280

uaccugcuca ccgaguuccu gaaccccgag uacgaggugg agaacgugcg cccguacagc     2340

cccucccagc aggacauccu gcggaucuac gaggagggcg uccucaacag cguguccgac     2400

cugcccgagg agaucagcga cauccugaag aaguucaccg agaccgaccu caagaagcgc     2460

cccucccgga gcgagauccu gcgcuacaag cuguggcucg agcagaagua ccggagcccg     2520

uacacggggg aggugauccc ccugggcaag cuguucaccc ccgcguacga gaucgagcac     2580

gucaucccgc aguccugcua cuucgacgac agcuucucca acaaggugau cugcgaggcc     2640

gaggugaaca agcucaagag caaccugcug ggccacgagu ucaucgucaa gcaccacggg     2700

gagaucgugg agcuccccuu cggcaagaag gugcgcaucu ucaccgucga cgaguacgag     2760

caguucguga aggacaacua cucccggacc cgcagcaaga ugaagaagcu gcugauggac     2820

gacauccccg aggaguucgu gacgcggcag cucaacgacu cccgcuacau cagcaagguc     2880

gugaaguccc ugcugagcaa caucgugcgg gagaaggggg aggaggaggc caucuccaag     2940

aacgucaucc ccugcaccgg cggcgugacc gaccgccuca agaaggacug ggggaucaac     3000

gaggugugga acaagaucau ccugccgcgg uuccagcgcc ugaacgagcu caccggcacg     3060

aacaaguuca ccaccaagaa cgucgggcug caggagaucc ccaccaugcc ccuggagcuc     3120

cagaagggcu ucaacaagaa gcggaucgac caccgccacc acgccaugga cgccaucauc     3180

aucgcgugcg ccaaccggaa caucaucaac uaccugaaca acgagagcgc caccgccaag     3240

gccgagcugu cccgcuacga ccuccagaag augcugugcg acaaggcgaa gacggacaac     3300

aacgggaacu acaagugggu gauccgcaag cccugggcca gcuucaccca ggacaccuac     3360

cuggcccucg agaacaucau cguguccuuc aagcagaacc ugcgggucau caacaaggcc     3420

accaaccgcu uccugcacua caacgaggag ggcaagaaga ucuucgugaa gcagggcaag     3480

ggggacaacu gggccauccg caagagcaug cacaaggaca cgguguucgg cgaggucaac     3540

cuccggcgga ucaagaccgu ggcgcugaac gaggccauga agaacccgca guccaucgug     3600

gucaaggacu ucaagcgcaa gcugcucgag cuguggaacc ugggguucga cgccaagcgg     3660

aucaagaagu acuucgagga caaccgcgag accuggagcg acaucaaccu cagcaagauc     3720

gagguguacu acuucuccaa ggacaccaag gaccgguucu ucgccacgcg caagccccug     3780

gacaccagcu ucgaccggaa gaagaucgag aacaacauca ccgacaccgg cauccagaag     3840

auccugcucc gccaccugga gcugaaggac aacaaccccg acaucgcguu cucccccgac     3900

gggaucgacg agaugaaccg gaacaucauc cagcucaaca acggcaagua ccaccagccg     3960

aucaucaagg ugcgcuggua cgagcaggcc gacaaguucg ccgucggcca gacggggaac     4020

aaguucagca aguucgugga ggccgccaag ggcaccaacc uguucuucgc gguguacgag     4080

uccaacaucc uggacaagaa gaccaacacc aucaucaaga agcggaacua cgccacgauc     4140

ccccucaacg ucgccaucga gcgccagaag caggggcugc ccguggcccc cgaggacgag     4200

aacggcaacg acccgaucuu cgugcugagc cccaacgacc ucgucuaccu gcccaccgac     4260

gacgagcugg ccaacgggau caucgcgcag ccccucgacc ggggccgcau cuacaagaug     4320

guguccagcu ccggcgagca gugcuucuuc aucaagcaca ucgucgccaa cgugcuggug     4380

gacaaguucg aguucagccc gcugaacaag auggagcggg cccucaccgg ggagaugauc     4440

aagaugaucu gcauccccau caaggucgac cgccugggca acgugcugga guccagcucc     4500

agccacaaga aguga                                                      4515


<210>  2257
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2257
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucgag       60

aacgacuuca accggaagga ggggaagauc gaggcccucg gcucccgcau cauccccaug      120

agccaggaca uccuggggaa guucgacucc ggcgugaccg ucagccagac ggcggcccgg      180

accaacuacc gcggcauccg gcgccuguac cagcgggaca accuccgccg ggagcgccug      240

caccgggugc ugaacauccu cggguuccug ccgaagcacu acgccgaguc caucgacuuc      300

gaggaccgcc ugggccaguu caagcccgag accgagguga agcuccccua ccggaaggac      360

agcgacgaca acuaccacuu caucuuccag gaguccuuca acgagauggu cgaggaguuc      420

caguucaaga accccagccu guucuacacc aaggccaacg gggagcaguc caagaucccg      480

uacgacugga cgcuguacua ccuccgcaag aaggcccuga gcaagaagau cuccaaggag      540

gagcuggcgu ggauccuccu gaacuucaac cagaagcggg gcuacuacca gcugcgcggg      600

gaggaggagg ccgacagcaa ggacaagcuc gaggaguacc acgcccugaa gguguccgac      660

gugcaggcca ccgaggacac caacgcgaag ggcaccuggu acaacgucau ccuggagaac      720

ggcuggaucu accggcgcca gagcaaggac ucccucuucg acuggaaggg gaagacgcgg      780

gaguucaucg ugaccaccag ccuggagaag ggcggggccg ugaagaucga caaggagggc      840

aacgagaagc gcuccuuccg ggccgucgac agcgagaagg acuggaucgc caucaagaag      900

aagaccgaga acgaccuggc caacgcgggc acgaccgugg ggguguacau cuacgagacc      960

cuccugcgca accccgacca gaagauccgc ggcaagcuga ucaagaccau cgagcggaag     1020

uacuaccucg aggagcugaa ggccauccug gccaagcaga uccaggagca cgccgagcuc     1080

cagaaccgcu cccuguacaa ggacugcauc gaggagcugu accaggggaa cgaggcccac     1140

aagaacaaca ucaaggagaa cggcuucgac uaccucuucc uggaggacau caucuucuac     1200

cagcggcccc ucaagagcaa gaaguccacg aucagcaacu gccccuacga gagccgcguc     1260

uucaucaagg acgggaagaa ggagauccag ccgaucaagu gcaucuccaa gagcaacccc     1320

aucuaccagg aguuccggcu guggcaguuc auccacaacc ugaagaucua cgagaaggag     1380

gcgaucgacg acggcaagac cgugaucaac aaggacguga ccaccgaguu ccugaacacg     1440

caggaggccu acgccgcccu cuucgaguuc cugaacgaca aggccgagau cucccagaac     1500

gcgcugcuca aguacuucaa gcugagcacc aacacccacc gcuggaacaa cgucgaggac     1560

aagaaguacc ccugcaacga gacccggucc gaguucauca agcgccugag caagauggac     1620

ggcaucgacc ucgggaagac gcuguccccc gccuucgaga agucccugug gcacaucauc     1680

uacagcguga aggacaagaa gcaguacgug cgggcccugg agaccuucgc caugaagaac     1740

ggccucgaca ucaaccaguu ccgcgagaac uucgagaagu ucaagccgua cgacagcgcc     1800

uacggggcgu acuccgagaa ggcccugaag aagguccugc cccucauccg gcgcggcacc     1860

uacuggaaga aggacgccau ccccgccgcg gugcaggagc ggaucgugaa caucaugaag     1920

cgcaucgaga gcgucgacuu caaccuggag aagaucgaga acuucgccga cgacgacauc     1980

cccaagcagc ugcucaaguc cuucgccaag gggaaggacc cgaugcaggg ccugaacacc     2040

uaccaggccu gcuacgccgu guacaaccgg cacagcgagg uguccgacau ccagcugugg     2100

aagucccccg aggacaucac gaaguaccuc gacagcuuca agcagcacag ccugcgcaac     2160

cccaucgucg agcaggucgu gauggagacc cugcgggugg ugcgcgacau cuggcaguac     2220

uacggcaacg gggcgaagga cuucuucagc gagauccacg ucgagcucgg ccgggagaug     2280

aagaacgaca agaagacccg cgagcgcauc uccaagagca ucaccgagaa cgagaacacg     2340

aacgagcgga ucaagaacau ccugaaggag cugauggggg acgaggcccu ccagggcgac     2400

auccgccccu acuccaaggg gcaccaggac auccugaagc uguacgagga gggcguguuc     2460

gccaacgccc cggagaccua caagaacauc aagaucgacg acaucgagaa gauccggaag     2520

aacaacagcc ccuccaagag cgacaucgug aaguacaagc ucuggcugga gcagggcuac     2580

aucucccccu acaccgggag ccccaucccg cugucccgcc ucuucaccac ggacuaccag     2640

aucgagcaca ucaucccccg gagccgcuac uucgacgacu ccaugggcaa caagaucauc     2700

ugcgagagcg ccgucaacga ggagaagacc aacaagaccg cguacgaguu caucaaggag     2760

aacgccgggc ggaucaucga ccugggccag ggcaagcagg ucccccuguu cgaccucgag     2820

gccuacgagu cccacugccg cggguacuuc aagggcaaca ccaagaagcu gaaguuccug     2880

cucagcgagg acauccccga gggguucauc aaccggcagc ugaacgacuc ccgcuacauc     2940

agcaaggugg ugaccucccu gcucagcaac guggugcggg aggagggcga gcaggaggcc     3000

acgagcaaga accugguccc gcuggugggg accaucaccu ccaagcucaa gaaggacugg     3060

ggccuggagg ccaucuggga cgaccugauc gcgccccgcu ucgagcggau gaaccagcuc     3120

accaagagca cggacuuccg caaggaggug aucgacggcc acgggaaccg guacuucauc     3180

aacaccgucc ccgacgacau caagaagggc uucuccaaga agcgcaucga ccaccggcac     3240

cacgcccugg acgcccuggu gaucgccugc accaccaagg accacgugaa cuacaucacg     3300

agccucaaca cccagcgcaa caaccggucc cuggucagca agcugcgcaa gaucgaggag     3360

aagaccauca aggacaagcg gaccgggcag ccgaagacga ucaagaucgc gaaggccuac     3420

cacaagcccu ggcccaacuu caccaaggag accuucgaga agcucuccaa gaccguggug     3480

agcuucaagc agaaccagcg caucaucaac aagacgaaca acaagaccug gggcugggag     3540

aaggucgacg gggugaugaa gaaggugcug aagaagcagg agaagcgcaa cugggccauc     3600

cggaagccca uccacaagga gaccuacuac ggcaagguca agggcuucga cuccccgaag     3660

gggaagauca ccacggccac ccgcaagagc cugcgggaga ucaccaacga gcgccagcuc     3720

aacaccauca cggacuccgg caucaagaag auccugaaca accaccugaa gaccuucauc     3780

gacgagagcg ggaagaucaa cuucgagccc gccuucaacc aggacggcau cgaccuccug     3840

aacaagaaca uccagcagcu gaacgagggg aagaaccacc agcccaucua caaggugcgg     3900

auguacaccc acaacaccau guucuccgug ggcgacgagg gcaacaagag cacgaaguac     3960

gucauccccg aguccgggac caaccucuuc uucaacgugu acugggacga ggagaacaac     4020

aagcgcaacu ucgagaccgu gccgcugaac cgggucaucg cguuccagaa gcagaccagc     4080

cacauccccc gcaaggaccg gacggacgug cccaucgaca acacccuggg ccgcuuccuc     4140

uucacccugu cccccgacga ccugguguac cucccgaccg acgaggagag cgaguccuuc     4200

acgaccuucg agaucgacag ccugaacagc aagcaggucg ccaacaucua caaguucgug     4260

gacggguccg gcaccaccgg gaacuucgug cccgccgagg ccgcccgggu caucgcgaac     4320

aucaaggaca agaaggugcg cagcaacuuc ugcaagcgga acaaccuguc cgacaagcag     4380

cucaucaaga acgaguucgg ccugggcagc ccccagcuga agaaccagaa cucccucgac     4440

gggcgccaga ucaagagcau cugcuggaag cuggagauca accggcuggg caagaucauc     4500

aaggugauca aguga                                                      4515


<210>  2258
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2258
augaagaagg ugcugggccu cgacaucggg accaacagcg ucggcugggc cguggugaac       60

cagaacgaca agggggaguu ccuguccauc gagaagaucg gcagccgcau cauccccaug      120

ucccaggacg ucauggacaa guucggggcc ggccagaccg agagcuccac ggcgcagcgg      180

acccaguacc gcggcgugcg gcgccugcgg gagcgcagcc uccugcggcg cgagcggcug      240

caccgcgugc uccacauccu gcacuuccug ccgcagcacu acgacgccgc caucgggugg      300

aacaagaagg agaacaagac cuacggcaag uuccuccccg gggucgaggu gaacaucccc      360

uggguggcca ccgaccaggg ccaccaguuc aucuucaugg acuccuacgc cgagaugcug      420

aaggaccuga agcagcacca gccccaccuc uucgagaagu ccagcacgcc ggucccccug      480

gacuggacca ucuacuaccu gcggaagaag gcgcucaccc aggccaucag caaggaggag      540

cuggccugga uccugcucaa cuucaaccag aagcgcgggu acuaccagcu gcggggcgag      600

gagguggagg acaacaccaa gcgcgaggag uucuacgccc ugaagguggu caagguggag      660

cucgacaagg acaacacguc cgcgaagacc ugguacaagg ugcaccugga gaacggcuuc      720

aucuaccccu gccccagcga caccccgauc gacuccuggg ucgggauggu gaaggacuuc      780

aucgugacca cgacccugga gaaggacggc agcgagaagc ggaccgccga cggggagauc      840

aagcgcuccu uccgggcccc caaggaggac gacuggaccc uccugaagaa gaagacggag      900

agcgagcugg agaaguccgg caagcagguc ggcgccuuca ucuacgacac ccuccugagc      960

aaccccgacc agaagaucau cgggggccug aucaagacca ucgagcgcaa guacuacaag     1020

aaggagcucg agcagauccu ggagacccag cagaaguuca ucgccgagcu guccgacaag     1080

aagcuguucc aggcgugcgc cgaggagcug uacccccuga acgacagcca ccgggagaac     1140

cuccuggagc gcgacuucaa gcaccuguuc gugcaggaca ucaucuucua ccaccggccg     1200

cucaagacga agaagucccu gaucagcaac ugcaccuacg aguaccgcac cuucuacaag     1260

aacggggaga agcagaucag ccccaucaag ugcaucgcca aguccaaccc ccuguuccag     1320

gaguuccgcg uguggcaguu cguccagaac cuccggaucc ugcagcgcca gcggagcgag     1380

ggcgacucca ucaaguacaa cguggacgug accgccgagu uccugaagga cgagaacgac     1440

aucgccaacc ucuucacgug gcugaacgag cgcaagguca ucaagcagga ccagcugcuc     1500

aagagcuacu ucaagcugaa ggugaacaag gagaccaagg agcuggacua ccgguggaac     1560

uacgucgacg acaagaacaa ggaguacccc ugcaacgcga cccgcaccca cauccugucc     1620

uuccucgaga agugcaagga ggagaagcgg aucgaggacg accgcuucgu guacagccug     1680

uggcaccucc ucuacuccgu ggucgagccg aucgagcugc gcggggcccu ggccaaguuc     1740

gccgagcggg agcaccucca ccccagcuuc guggacgugu ucgcccaguu cccgccccug     1800

gagaaggagu acggcuccua cagcgagaag gcgaucaagc ggcugcuccc ccugaugcgc     1860

augggcgacu acuggcacga ggacaagauc gacgucaaga cgaagcagcg gauccagcgc     1920

aucaucgacg gggaggccga cgacgacauc acccaccggu cccgcgagaa gguggagaac     1980

cugcggagca ucaccgacuu caagggccuc ccccugugga aggccgggua cguggucuac     2040

aaccgccacu ccgagagcgg cgagcugacc aaguggaaga cgccccacga gcucuccaac     2100

uacauccaga acgaguucaa gcaguacagc cugcggaacc ccaucgugga gcagaucgug     2160

cuggagaccc uccgcgucgu gcacgaccug ugggugaagc acggggacuu cuccgagauc     2220

caccuggagc ucggccgcga caucaagaac agcagcgaca agcggaagcg cauggccgag     2280

cggcagcagg agaacgagaa cucccgccag cggguccugg agaugcugau cgaccucaag     2340

aaggaggccg cguucgccga ccugaacccg uacagccccu cccagcagga gaagcugcgc     2400

aucguggagg acggcgugcu ccggagcaac aucgagaucc ccaaggacau cgagaagauc     2460

accaaguccg ccaagcccac cagccgcgag cugacgaagu accggcugug gcucgaccag     2520

aaguaccgcu ccccguacac cgggcagccc aucagccugg cccagcuguu caccuccaag     2580

uacgagaucg agcacgucau cccccaggcg aaguacuucg acgacagcuu caccaacaag     2640

gugaucugcg aguccgaggu gaacaaggag aagggcagca ugcucgccca cgaguucauc     2700

cugaagaacg ggggcuccac cgucaacgug ggcgggcgcc aggugcaggu ccugaagaac     2760

gacgccuacg uggagcucau caaccggcug uacggcaaga acaacccggu gaaggccaag     2820

aagcugcucg ccgaggacau cgacgagguc gugggggacu ucagcgagcg gcagcugaac     2880

gacacgcgcu acaucucccg ggugguccag aagcugcuca gcaacaucgu gcgcgaggag     2940

gacgagcagg aguccaccag caagcacgug aucggcugca gcgggcaggu caccaccgcg     3000

cugaagaagg acuggggccu gaacgacauc uggaacaaga ucauccagcc ccgguucgag     3060

cgccucaacg agaugacggc caccgaggac uacggcgugu ggcagucccc caaccacuuc     3120

cagauccggg ugcccgagga gauccagaag ggguucacca agaagcgcau cgaccaccgg     3180

caccacgcca uggacgcccu ggucaucgcc ugcgcgaccc gcagccacau caacuacauc     3240

uccaaccgca gcggccagga ccggaacgcc aaguucgaga cgacccgcac cgaccugcgg     3300

uccaagcucu gcuacaagga gaacagcucc gggaacaacu acaacuacau cuucaacaag     3360

ccgugggaca ccuucacgca ggacgcccac cgcgcccugg agaacaucgu ggugagccac     3420

aagcagaacc ugcggguccu caccaagacc aacaacuaca cccaguucau caacaaggac     3480

ggcaagaagg accugcagaa gcaggucaag ggggaccacu ucgccgugcg caagcccaug     3540

caccaggaga cgaucuacgg ccgggugucc auccgcagca ucaaggaggu ggcgcugaag     3600

aaggcccucg agaacuggaa gcagaucgug gaccggaccc ugaaggacaa gauccgcgag     3660

cugaucggcg ccuacgggaa guacgacgcc cagaccaucc ucaaguacuu caaggaccgg     3720

gacuacaagu ucaacaaccu gaagaucaac cgcgucgagg uguacgagua cgaccacaac     3780

ugcgcggccg ugcggaaguc caucggcgac gaccugacca ucaagcagau cgagcgcauc     3840

acggacgaga gcauccggaa gauccucaug aaccaccugu ccaacuacga cgacaaccug     3900

aaggaggccu ucagccccga ggggcucaug gagaugaaca agaacaucaa ggagcugaac     3960

ggcgggaaga accaccagcc caucuacaag guccgccugu ucgaggaccu cggcuccaag     4020

uucccgcugg gcgugagcca caacaacccc gacaaguacg ccaucgccgc gaaggggacc     4080

aaccuguucu ucggcaucua cgaggaccag gaggggaagc ggauguucga cuccaucccc     4140

cucaacaucg ugaucgaccg ccugaaggag aagaaguccc ccgucccgga gaccuacaac     4200

gagagcaagc ugaaguucca ccucagcccc aacgaccugg uguacguccc caccgacgag     4260

gagcagaagc agcccgagcu gguggacgug gaccaccuca gcgacgagca gaagggccgg     4320

gucuacaagg ccguguccuu cacggggaac cgcgccuucu ucgugaacca ccgcgucagc     4380

aaggugaucc acgacuacgu ggaguucggc accaagaaca agaucgagaa cgacgacgac     4440

aagcgguccc ugaagcacau cugcuggaag cugaagguca accgccucgg cgagaucacc     4500

gacgugaagc gguga                                                      4515


<210>  2259
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2259
augaagaaca ucgugggccu ggaccucggg accaacagca ucggcugggc cgucgugaac       60

ggguccguga acgacgacgg cagcgagcag cuggucaaga uccaggccuc cgggagccgc      120

aucaucccca uggacgcggc caugaucggc gacuucaaca agggcaacuc caucagccag      180

accgccgagc ggacgcgccu gcgcggggug cggcggcucu ccgagcgcua ccugcugcgg      240

cgcgagcggc uccaccgcau ccuggacauc cugggcuucc ucccguucca cuucgcccag      300

gaccuggacc ggcacgggaa gaucgugaag ggcaaggagc ccaagcuggc cuggcgcaag      360

aacgaggcgg ggcaguucga guucaucuuc caggacagcu ucaaggagau gcucgaggac      420

uucaagcuga acuaccccaa ccugaucacc gacgacaaga agguccccua cgacuggacc      480

aucuacuacc uccggaagaa gggccugacc uccaagauca gcaaggagga gcuggccugg      540

auccuccuga acuucaacca gaagcgcggc uacuaccagc ugcgcgggga ggaggaggag      600

gagaacaagg acaagcucgu ggaguucuac gcccugaagg uggucgccgu ggaggacucc      660

ggcgagaaga aggggaagga caucugguac aacgugcacc uggagaacgg cugggucuac      720

cggcggacga gcaacauguc ccucgacugg gugggcaaga ccaaggaguu caucgugacc      780

accgaccugg agaaggacgg gacgccgaag aaggacaagg agggcaacgu caagcgcagc      840

uuccgggcgc ccaaggagga cgacuggucc cuggugaaga agaagaccga ggccgacauc      900

gaccagaccc acaagaccgu gggggccuac aucuacgaca cgcuccuggg caaccccagc      960

cagaagaucc gcgggcggcu gguccgcacc aucgagcgca aguacuacaa ggaggagcuc     1020

aagcugaucc uggagaagca gaaggaguuc caccucgagc ugcaggaccg ggcccuguac     1080

gccgcgugca ucgaggagcu cuaccccauc aacgaggccc accgcaacaa cgugggcaac     1140

cgggacuuca ccuaccuguu ccuggaggac auccucuucu accagcgccc gcugaagucc     1200

aagaaguccc ugaucgacaa cugccccuac gaggagaacc agggccucga caaggagacc     1260

ggggagauca agagcuuccc cgugaagugc aucgccaaga gccacccccu guuccaggag     1320

uuccggcugu ggcaguucau caucaaccuc cgcaucuacc ggaaggaccu ggcccaggac     1380

gucgacguga cgaacgagcu gcucaagacc gaggaggacu acguggcccu guucgacugg     1440

cugaacggca agaaggagau cgaccagaag gcguuccuga aguacccggu cuucgggcuc     1500

aagaaggaga ucgagaacua ccgcuggaac uacgugcagg acaagcccua ccccugcaac     1560

gagacccgga gccugaugcu ggcccgccuc gagaagugcg acaucuccau cgaguuccug     1620

accaaggaga acgaggaggc ccuguggcac auccucuaca gcgucgagga caagaacgag     1680

aucgugaagg cccugcagaa guucgccgac aagcacgacc ugaacgcguc cuucguggac     1740

gucuuccaga aguucccgcc cuucaagaag gaguacggca gcuacuccgc caaggccauc     1800

aagaagcucc ugccccugau gcggaugggg aaguacugga gcgagaacuc caucgacaag     1860

ggcacgcgcg agcggaucga caagaucauc accggcgagu acgacgagaa gaucgagaac     1920

cgcgugcggg agaaggccau ccaccucgcc gacgugagcg acuucaaggg gcugccccug     1980

uggcucgucu gcuacaucgu guacggccgc cacuccgagg cgaaggagau cauccagugg     2040

aagucccccg ccgacaucga cagcuaccug cggagcuuca agcagcacuc ccugcgcaac     2100

ccgaucgugg agcagaucau caucgagacc cuccgggucg ugcgcgacau cuggaagcag     2160

gucgggaaca ucgacgagau ccacguggag cugggccgcg agaugaagaa ccccgccgac     2220

aagcggaaga agaugaccca gcagaugagc gagaacgaga acacgaaccu gcgcaucaag     2280

uaccuccuga ccgaguuccu gaaccccgag uacgaggugg agaacgugcg gcccuacucc     2340

ccgagccagc aggacauccu ccgcaucuac gaggaggggg uccugaacuc cgugagcgac     2400

cugcccgagg agaucuccga cauccucaag aaguucaccg agaccgaccu gaagaagcgg     2460

cccagccgca gcgaggugcu gcgguacaag cucuggcugg agcagaagua ccgcuccccc     2520

uacacgggcg aggucauccc gcugggcaag cuguucaccc ccgccuacga gaucgagcac     2580

gugauccccc agagcuacua cuucgacgac uccuucagca acaaggugau cugcgaggcg     2640

gaggucaaca agcucaaguc caaccugcuc gggcacgagu ucaucgugaa gcaccacggc     2700

gagaucgugg agcugccguu cgggaagaag guccggaucu ucaccgugga cgaguacgag     2760

caguucguga aggacaacua cagccgcacc cgguccaaga ugaagaagcu gcucauggac     2820

gacauccccg aggaguucgu cacgcgccag cugaacgaca gccgguacau cuccaaggug     2880

gucaagagcc ugcucuccaa caucgugcgc gagaagggcg aggaggaggc caucagcaag     2940

aacgugaucc ccugcaccgg cggggugacc gaccggcuga agaaggacug gggcaucaac     3000

gaggucugga acaagaucau ccugccccgc uuccagcggc ucaacgagcu gaccgggacg     3060

aacaaguuca ccaccaagaa cgugggcauc caggagaucc cgaccaugcc ccuggagcuc     3120

cagaaggggu ucaacaagaa gcgcaucgac caccggcacc acgccaugga cgccaucauc     3180

aucgccugcg cgaaccgcaa caucaucaac uaccugaaca acaaguccgc caccgccaag     3240

gccgagcuga gccgguacga ccuccagaag augcugugcg acaaggccaa gacggacaac     3300

aacggcaacu acaagugggu gauccgcaag cccugggcgu ccuucaccca ggacaccuac     3360

cuggcccucg agaacaucau cgucagcuuc aagcagaacc ugcgcgugau caacaaggcc     3420

accaaccggu uccugcacua caacgaggag ggcaagaaga ucuucgugaa gcaggggaag     3480

ggcgacaacu gggccauccg caaguccaug cacaaggaca cggucuucgg ggaggugaac     3540

cuccggcgca ucaagaccgu ggcccugaac gaggcgauga agaaccccca gagcaucguc     3600

gugaaggacu ucaagcggaa gcugcucgag cuguggaacc ugggcuucga cgccaagcgc     3660

aucaagaagu acuucgagga caaccgggag accuggagcg acaucaaccu cuccaagauc     3720

gagguguacu acuucagcaa ggacaccaag gaccgcuucu ucgccacgcg gaagccgcug     3780

gacaccuccu ucgaccgcaa gaagaucgag aacaacauca ccgacaccgg gauccagaag     3840

auccugcucc ggcaccugga gcugaaggac aacaaccccg acaucgccuu cagccccgac     3900

ggcaucgacg agaugaaccg caacaucauc cagcucaaca acggcaagua ccaccagccc     3960

aucaucaaga uccgguggua cgagcaggcg gacaaguucg ccgucgggca gacgggcaac     4020

aaguccagca aguucgugga ggccgccaag gggaccaacc uguucuucgc cguguacgag     4080

uccaacaucc uggacaagaa gaccaacacc aucaucaaga agcgcaacua cgcgacgauc     4140

ccgcucaacg ucgccaucga gcggcagaag cagggccugc ccguggcccc cgaggacgag     4200

aacgggaacg accccaucuu cgugcugagc ccgaacgacc ucgucuaccu gcccaccgac     4260

gacgagcugg ccaacggcau caucgcccag ccccucgacc gcggccggau cuacaagaug     4320

guguccagcu ccggggagca gugcuucuuc aucaagcaca ucgucgcgaa cgugcuggug     4380

gacaaguucg aguucagccc ccugaacaag auggagcgcg cccucaccgg cgagaugauc     4440

aagaugaucu gcaucccgau caaggucgac cggcugggga acgugcugga guccagcucc     4500

agcuacaaga aguga                                                      4515


<210>  2260
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2260
augaagaaca ucgugggccu ggaccucggg accaacagca ucggcugggc cgucgugaac       60

ggguccguga acgacgacgg cagcgagcag cuggucaaga uccaggccuc cgggagccgc      120

aucaucccca uggacgcggc caugaucggc gacuucaaca agggcaacuc caucagccag      180

accgccgagc ggacgcgccu gcgcggggug cggcggcucu ccgagcgcua ccugcugcgg      240

cgcgagcggc uccaccgcau ccuggacauc cugggcuucc ucccguucca cuucgcccag      300

gaccuggacc ggcacgggaa gaucgugaag ggcaaggagc ccaagcuggc cuggcgcaag      360

aacgaggcgg ggcaguucga guucaucuuc caggacagcu ucaaggagau gcucgaggac      420

uucaagcuga accaccccaa ccugaucacc gacgacaaga agguccccua cgacuggacc      480

aucuacuacc uccggaagaa gggccugacc uccaagauca gcaaggagga gcuggccugg      540

auccuccuga acuucaacca gaagcgcggc uacuaccagc ugcgcgggga ggaggaggag      600

gagaacaaga acaagcucgu ggaguucuac gcccugaagg uggucgccgu ggaggacucc      660

ggcgagaaga aggggaagga caucugguac aacgugcacc uggagaacgg cugggucuac      720

cggcggacga gcaacaugcc gcucgacugg gugggcaaga ccaaggaguu caucgugacc      780

accgaccugg agaaggacgg gacgcccaag aaggacaagg agggcaacgu caagcgcucc      840

uuccgggcgc ccaaggagga cgacuggagc cugcucaaga agaagaccga ggccgacauc      900

gaccagaccc acaagaccgu ggggguguac aucuacgaca cgcugcuggg caaccccucc      960

cagaagaucc gcgggcggcu cguccgcacc aucgagcgca aguacuacaa ggaggagcug     1020

aagcagaucc uggagaagca gaaggaguuc caccucgagc ugcaggaccg ggcccuguac     1080

gccgccugca ucgaggagcu cuacccgauc aacgaggcgc accgcaacaa cgugggcaac     1140

cgggacuuca ccuaccuguu ccuggaggac auccucuucu accagcgccc ccucaagagc     1200

aagaaguccc ugaucgacaa cugccccuac gaggagaacc agggccucga caaggagacc     1260

ggggagauca agagcuuccc cgugaagugc aucgccaagu cccacccgcu guuccaggag     1320

uuccggcugu ggcaguucau cgucaaccuc cgcaucuacc ggaaggaccu ggcccaggac     1380

guggacguga cgaacgagcu gcucaagacc gaggaggacu acgucgcccu guucgacugg     1440

cugaacggca agaaggagau cgaccagaag gccuuccuga aguaccccgu guucgggcuc     1500

aagaaggaga ucgagaacua ccgcuggaac uacguccagg acaagcccua ccccugcaac     1560

gagacccgga gccugaugcu ggcgcgccuc gagaagugcg acaucagcau cgaguuccug     1620

accaaggaga acgaggaggc ccuguggcac auccucuacu ccguggagga caagaacgag     1680

aucgugaagg cccugcagaa guucgccgac aagcacgacc ugaacgccag cuucgucgac     1740

guguuccaga aguucccgcc cuucaagaag gaguacggcu ccuacagcgc gaaggccauc     1800

aagaagcucc ugccccugau gcggaugggg aaguacuggu ccgagaacag caucgacaag     1860

ggcacgcgcg agcggaucga caagaucauc accggcgagu acgacgagaa gaucgagaac     1920

cgcgugcggg agaaggccau ccaccucgcc gacgucuccg acuucaaggg gcugccccug     1980

uggcucgugu gcuacaucgu guacggccgc cacagcgagg ccaaggagau cacccagugg     2040

aaguccccgg cggacaucga cagcuaccug cgguccuuca agcagcacag ccugcgcaac     2100

cccaucgucg agcagaucau caucgagacc cuccgggugg ugcgcgacau cuggaagcag     2160

gucgggaaca ucgacgagau ccacguggag cugggccgcg agaugaagaa ccccgccgac     2220

aagcggaaga agaugacgca gcagaugucc gagaacgaga acaccaaccu gcgcaucaag     2280

uaccuccuga ccgaguuccu gaaccccgag uacgaggugg agaacguccg gccguacagc     2340

cccucccagc aggacauccu ccgcaucuac gaggaggggg ugcugaacag cguguccgac     2400

cugcccgagg agaucagcga cauccucaag aaguucaccg agacggaccu gaagaagcgg     2460

cccucccgca gcgagauccu gcgguacaag cucuggcugg agcagaagua ccgcagcccg     2520

uacaccggcg aggucauccc ccugggcaag cuguucaccc ccgccuacga gaucgagcac     2580

gugaucccgc aguccugcua cuucgacgac agcuucucca acaaggugau cugcgaggcc     2640

gaggucaaca agcucaagag caaccugcug gggcacgagu ucaucgugaa gcaccacggc     2700

gagaucgugg agcugcccuu cgggaagaag guccggaucu ucaccgugga cgaguacgag     2760

caguucguga aggacaacua cucccgcacg cggagcaaga ugaagaagcu gcucauggac     2820

gacauccccg aggaguucgu cacccgccag cugaacgacu cccgguacau cagcaaggug     2880

gugaaguccc ugcucagcaa caucguccgc gagaagggcg aggaggaggc gaucuccaag     2940

aacgugaucc ccugcaccgg cggggugacc gaccggcuga agaaggacug gggcaucaac     3000

gaggucugga acaagaucau ccugccgcgc uuccagcggc ucaacgagcu gacggggacc     3060

aacaaguuca ccaccaagaa cgugggcauc caggagaucc ccaccaugcc ccuggagcuc     3120

cagaaggggu ucaacaagaa gcgcaucgac caccggcacc acgccaugga cgccaucauc     3180

aucgccugcg ccaaccgcaa caucaucaac uaccugaaca acgagagcgc gacggccaag     3240

gccgagcugu cccgguacga ccuccagaag augcugugcg acaaggccaa gaccgacaac     3300

aacggcaacu acaagugggu gauccgcaag cccugggcca gcuucaccca ggacaccuac     3360

cuggcgcucg agaacaucau cgucuccuuc aagcagaacc ugcgcgugau caacaaggcc     3420

acgaaccggu uccugcacua caacgaggag ggcaagaaga ucuucgugaa gcaggggaag     3480

ggcgacaacu gggccauccg caagagcaug cacaaggaca ccgucuucgg ggaggugaac     3540

cuccggcgca ucaagaccgu ggcccugaac gaggccauga agaacccgca guccaucguc     3600

gugaaggacu ucaagcggaa gcugcucgag cuguggaacc ugggcuucga cgcgaagcgc     3660

aucaagaagu acuucgagga caaccgggag accuggagcg acaucaaccu cagcaagauc     3720

gagguguacu acuucuccaa ggacacgaag gaccgcuucu ucgccacccg gaagccccug     3780

gacaccagcu ucgaccgcaa gaagaucgag aacaacauca ccgacacggg gauccagaag     3840

auccugcucc ggcaccugga gcugaaggac aacaaccccg acaucgccuu cucccccgac     3900

ggcaucgacg agaugaaccg caacaucauc cagcucaaca acggcaagua ccaccagccg     3960

aucaucaaga uccgguggua cgagcaggcc gacaaguucg cggucgggca gaccggcaac     4020

aaguucagca aguucgugga ggccgccaag gggaccaacc uguucuucgc cguguacgag     4080

uccaacaucc uggacaagaa gaccaacacg aucaucaaga agcgcaacua cgccaccauc     4140

ccccucaacg ucgcgaucga gcggcagaag cagggccugc ccguggcccc cgaggacgag     4200

aacgggaacg acccgaucuu cgugcugagc cccaacgacc ucgucuaccu gcccaccgac     4260

gacgagcugg ccaacggcau caucgcccag ccccucgacc gcggccggau cuacaagaug     4320

guguccugca ccgggaacga gggccacuuc aucccggccc gcaucgcgaa ccccauccug     4380

cagacgaucg agcuggggag caacaacaag gcccagaagg ccuggaccga cgagaugauc     4440

aaggagaucu gcaugcccau caaggucgac cggcucggca acgugcugga guccagcucc     4500

agcuacaaga aguga                                                      4515


<210>  2261
<211>  4521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2261
augaagacca uccugggccu cgaccugggg cccaacagca ucggcugggc caagguggag       60

gucgacgacg aggggaacua ccugcgcaac aucaagcucg gcucccggau caucccgaug      120

acccaggaca ugcuggggga cuucgacaag ggcgugaagg ugagccagac ggccauccgc      180

accgacuucc ggggcaaccg ccggcugauc gagcgccacc uccugcggcg cgagcggcug      240

caccgcgucc uccacgcgcu gaaguuccug cccaagcacu acgaggacgc caucgggugg      300

gaccggaacg agcccgccac cuacggcaag uucgugaaca acuccgagcc caagcucgcc      360

ugggagcgcc aggaggacgg gagcaugcgg uuccuguuca uggaguccuu caacgagaug      420

guggccgacu ucgcgcagaa ccagccgagc cuggucuccg acggcaagaa gaucccccuc      480

gacuggacca ucuacuaccu gcgcaagaag gcccugacgc ggcccaucaa gaaggaggag      540

cucgccugga uccugcugaa cuucaaccag aagcgcgggu acuaccagcu ccggggcgag      600

gaggaggagg acaaccugac caagcgcaag gaguacuaca agcugaaggu ggugggcguc      660

gaggccgacg gggcgcccaa gggcgggaag accugguaca acauccaccu cgagaacggc      720

uggaccgccc ggugccagag caagaucccg cuggacgacu gggugggcaa ggugaaggag      780

uacaucguca cgaccgagua cgaggacgac gggaagaccc ccaagugcga caaggagggc      840

aaggugaagc gcuccuucaa gagccccgag aaggacgacu ggcagcugau caagacccgg      900

acggagaagu ucaucgacga cuccaacaag accguggggg ccuucaucua cgacuaccuc      960

cuggagaagc ccuucgacaa gauccgcggc aaguacaucc gcaccaucga gcggaaguac     1020

uacaagaacg agcugcgcga gauccuccgg gagcagacca aguaccacga ggggcugcac     1080

agccuggagg cccucaagau cugcgccgag gagcuguaca agaacaacca gcagcaccag     1140

gaguaccugc ucaagaagga caugacguuc cugcugcuca acgaccugau cuucuaccag     1200

cgcccgcuga aguccaagaa gucccucauc gcggacugcc ccuacgaggu cuacaaguac     1260

gacgacaagc ugaccggcaa gaagggccag aagaagauca agugcaucgc caagagcaac     1320

cccuacuacc aggaguuccg gcuguggcag uucaucgcca accuccgccu guucagcgug     1380

accgacggga aggaggugac cgccgaguac cugaagacgg aggaggacua cguccgccuc     1440

uucuccuucc ugaacgaccg gaaggacauc aagcaggaga ccuuccugcg gagcuuccug     1500

aagcucaaga aggaguccau cggcagcgag aagaucuacc ccauccgcug gaacuacauc     1560

gaggacgcca acaagagcua cccgugcaac gcgacccggc acgagcugcu gcucgccuuc     1620

gaccgcgccg acaucccccg ggagcugcug gacguggagg agcgcgagua ccggcucugg     1680

caccuccugu acuccaucga ggccaaggag gagaccgaga gcgcccuccg caagcugaag     1740

gcgaacaagg ccuucgggga ccugaacauc gacgacgccu ucgugaacuc cuuccucaag     1800

aucaagcccu ucgagaagga guacggcgcc uacagcgaga aggccaucaa gaagcugcug     1860

gcggucaugc ggcgcggguc ccucuggaag gaggccgaca ucugcgaggc cacgaagcag     1920

aacaucgcca agauccugca gaacgacauc gacgagaagc ugaagaagaa gaucgaggcg     1980

gccgugagcu ccuucaacaa gcucagcgac uuccagggcc ugcccaccug gcuggccugc     2040

uacguggucu acggccggca cuccgaggcc gcccagaucc agugcuggca gagcccggag     2100

gaccucaaga ccuacaucaa caaguucaag cagcacuccc ugcgcaaccc caucguggag     2160

cagugccugc ucgagacccu gcgcacgguc uacgacaucu ggaaggacgc ggggcagauc     2220

gacgagaucc acaucgagcu gggccggaac augaagucca ccgccgagca gcgcgcccgg     2280

aagaccaagg acauccuccg caacgagaac accaaccugc ggaucaagaa ccugcucgug     2340

gagcugaaga acgacgacaa gaucgagaac gugcgccccu acagccccag ccagcaggag     2400

auccugcgga ucuacgagga gggggcccuc caggagcugu ccaaggagga cgagauguuc     2460

gaggacauca gcaagaucuc caagauggcc cagccgagcc ccacggagcu gucccgcuac     2520

aagcucuggc uggagcagaa guaccggagc cccuacaccg gccgcgucau cccccugagc     2580

aagcuguuca ccaccgcgua ccagaucgag cacaucaucc cgcagucccg guacuucgac     2640

gacagcuuca acaacaaggu gaucugcgag uccgagguga acagccugaa ggacaacaug     2700

cugggguacg aguucaucaa ggagugcggc ggccgcaucg uccacugcac gcuccugggg     2760

gacgugaaga ucuucaccaa ggaggaguac aaggacuucg ugaccaagca cuacaucgac     2820

aacaccuaca agcgggacaa gcugcuccug gaggacaucc cccaggaguu cuccgcccgc     2880

cagcugaacg acagccggua caucuccaag augguccaga ccgugcucag caacaucgug     2940

cgcgccgaga acgagcagga ggccacgucc aaguucgucg ugagcugcaa cggcgccauc     3000

accaaccggc ugaagaagga cugggggcug aacgacgugu gguccagcau cgucugcccc     3060

cgcuucaagc ggcucaacga gaucaccaag accaacgcgu ucggccacug ggaggacaag     3120

gccggcaagc acguguucca gacgaccgug ccgcuggagc ugcagcgcgg guucuccaag     3180

aagcggaucg accaccgcca ccacgccaug gacgcccucg ucaucgcgug cgccagccgc     3240

aacaucguga acuaccugaa caacgagucc gccaacagcc ccgagaagcg ggaggaccug     3300

cgcaagaagc ucugcgacga gaagcggaag auccgcaagc ccuggaagac cuucacccag     3360

gacgcccggg aggcccuguc cgacgugguc gugagcuuca agaacuacgu gcgcgucauc     3420

aacaaggcga cgaacuacua cgagcacuac agcuccagcg gcaacaaggu gaugaucucc     3480

cagaagggga acgacaugug ggccauccgg aagccccugc acaaggagag cguguucggc     3540

cgcgucaacc ucugccggaa ggugaucgug ucccugaaga aggcccugga gaacaucccg     3600

gccaucugcg acaagacccu ccgcgacuac aucaacgccc uggucgagaa gcacuucaac     3660

acccggcagc ugcucgcgca cuucaagagc aucaacuacc gccugaaccg gcagugcgug     3720

gagaaggugg aggucuggca guucuccgac gagaagaucg agaugguggc ccgccgcaag     3780

agccuggaca ccuccuucga cgacaagcag aucagcucca ucacggacac cgggauccag     3840

aagauccucc ugaacuaccu gcagaccaag gacggcaacg ccgagaucgc cuucaccccc     3900

gagggcaucg aggagaugaa caagaacauc agccucuaca acgacgggaa ggcccaccag     3960

cccauccuga aggugcgggu cgcgacgccc aagggcauga aguacccggu ggggcagacc     4020

ggcaacaagg ccaccaaguu cguggucgcc gagaagggga ccaaccugua cuucgccauc     4080

uacgagaacg agcucggcgu gcgguccuac gacacgaucc cccugaacau cguggucgag     4140

cgccugaagg agggccucag ccccgugccc caggcgaacg aggccgacau cccgcugaag     4200

uucuaccugu cccccaacga ccucguguac guccccacca aggagaacga ggacgagcug     4260

ugccgggacc gcaucuacaa guucguggac agcuccggga ccaccgccaa cuucgugccc     4320

cacacgagcg ccaacaccau cuacaacguc gacaaggagu acgccgagaa gugcugcaac     4380

ggcgcggugu uccugaacga guacgggcuc ggcuccccgc agagcaagaa ccagcgcgcc     4440

cugaccgggg agaugaucaa ggagaucugc uggaagcuga agguggaccg gcucggcaac     4500

aucaucaagg ucaucaagug a                                               4521


<210>  2262
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2262
augaagaaca ucgugggccu ggaccucggg accaacagca ucggcugggc cgucgugaac       60

ggguccguga acgacgacgg cagcgagcag cuggucaaga uccaggccuc cgggagccgc      120

aucaucccca uggacgcggc caugaucggc gacuucaaca agggcaacuc caucagccag      180

accgccgagc ggacgcgccu gcgcggggug cggcggcucu ccgagcgcua ccugcugcgg      240

cgcgagcggc uccaccgcau ccuggacauc cugggcuucc ucccguucca cuucgcccag      300

gaccuggacc ggcacgggaa gaucgugaag ggcaaggagc ccaagcuggc cuggcgcaag      360

aacgaggcgg ggcaguucga guucaucuuc caggacagcu ucaaggagau gcucgaggac      420

uucaagcuga accaccccaa ccugaucacc gacgacaaga agguccccua cgacuggacc      480

aucuacuacc uccggaagaa gggccugacc uccaagauca gcaaggagga gcuggccugg      540

auccuccuga acuucaacca gaagcgcggc uacuaccagc ugcgcgggga ggaggagaag      600

aagaacgaca acgugaugau caucaacucc aaggugaucg acgucgugaa ggccgagaag      660

gacaagaagu acgagaagua cugguacaac gugcuccugg agaacggccu gcucuaccgg      720

gccgcguucu acaacgacau cagcuucugg aaggggcaca cgaaggaguu cguccugaag      780

accaucaagc ugaaguccgg cggcaccaag caggagcuca gcuuccugcc guccuucgac      840

gagaucgaga agauggagaa gaagcagaag gacaagaugu acuucaagau caagcugaag      900

accgaggccg acaucgacca ggcccacaag acggugggga ccuacaucua cgacacccuc      960

cugggcaacc ccagccagaa gauccggggg cgccuggugc ggaccaucga gcgcaaguac     1020

uacaaggagg agcucaagcu gauccuggac aagcagaagg aguuccaccc cgagcuccag     1080

gaccgggccc uguacgccgc gugcaucgag gagcuguacc ccaucaacga ggcccaccgc     1140

aacaacgucg gcaaccgcga cuucacguac cucuuccugg aggacauccu guucuaccag     1200

cggccgcuca aguccaagaa gucccugauc gacaacugcc ccuacgagga gaaccagggg     1260

cuggacaagg agaccggcga gaucaagagc uuccccguga agugcaucgc caagagccac     1320

ccccucuucc aggaguuccg ccuguggcag uucaucguga accugcggau cuaccgcaag     1380

gaccucgccc aggacgucga cgugaccaac gagcugcuga agaccgagga ggacuacgug     1440

gcccuguucg acuggcucaa cggcaagaag gagaucgacc agaaggcguu ccugaaguac     1500

ccggucuucg ggcugaagaa ggagaucgag aacuaccggu ggaacuacgu gcaggacaag     1560

cccuaccccu gcaacgagac gcgccuccug augcuggccc ggcucgagaa gugcgacauc     1620

agcaucgagu uccugaccaa ggagaacgag gaggcccugu ggcacauccu cuacuccgug     1680

gaggacaaga acgagaucgu caaggcccug cagaaguucg ccgacaagca cgaccugaac     1740

gcgagcuucg uggacgucuu ccgcaaguuc ccgcccuuca agaaggagua cggcuccuac     1800

agcgccaagg ccaucaagaa gcuccugccc cugaugcgga uggggaagua cugguccgag     1860

gacagcaucg acaagggcac ccgcaagcgg auggacaaga ucaucaccgg ggaguacgac     1920

gagaagaucg agaaccgcgu gcgggagaag gugauccacc ucgccgacgu cuccgacuuc     1980

cgcggccugc cccuguggcu cgugugcuac aucguguacg gccggcacag cgaggccaag     2040

gagaucacgc aguggaaguc ccccgcggac aucgacagcu accugaaguc cuucaagcag     2100

cacagccugc gcaacccgau cgucgagcag aucaucaucg agacccuccg gguggugcgc     2160

gacaucugga agcaggucgg gaacaucgac gagauccacg uggagcuggg ccgggagaug     2220

aagaaccccg ccgacaagcg caagaagaug acccagcaga uguccgagaa cgagaacacc     2280

aaccugcgca ucaaguaccu ccugacggag uuccugaacc ccgaguacga gguggagaac     2340

guccggcccu acagcccguc ccagcaggac auccuccgca ucuacgagga gggggugcug     2400

aacagcgugu ccgaccugcc cgaggagauc agcgacaucc ucaagaaguu caccgagacc     2460

gaccugaaga agcggcccuc ccgcagcgag auccugcggu acaagcucug gcuggagcag     2520

aaguaccgca gccccuacac cggcgagguc aucccgcugg ggaagcuguu cacgcccgcc     2580

uacgagaucg agcacgugau cccccagucc ugcuacuucg acgacagcuu cuccaacaag     2640

gugaucugcg aggccgaggu caacaagcuc aagagcaacc ugcucggcca cgaguucauc     2700

gugaagcacc acggcgagau cguggagcug ccguucggga agaagguccg gaucuucacc     2760

guggacgagu acgagcaguu cgugaaggac aacuacuccc gcacccgggg caagaugaag     2820

aagcugcuca uggacgacau ccccgaggag uucgucgcgc gccagcugaa cgacagccgg     2880

uacaucucca agguggucaa gagccugcuc uccaacaucg ugcgcgagaa gggggaggag     2940

gaggccauca gcaagaacgu gauccccugc accggcggcg ugacggaccg gcugaagaag     3000

gacuggggga ucaacgaggu cuggaacaag aucauccugc cccgcuucca gcggcucaac     3060

gagcugaccg gcaccaacaa guucaccacg aagaacgugg ggauccagga gaucccgacc     3120

augccccugg agcuccagaa gggcuucaac aagaagcgca ucgaccaccg gcaccacgcc     3180

auggacgcca ucaucaucgc cugcgcgaac cgcaacauca ucaacuaccu gaacaacgag     3240

uccgccaccg ccaaggccga gcugagccgg uacgaccucc agaagcugcu gugcgacaag     3300

gccaagaccg acaacaacgg gaacuacaag ugggugaucc gcaagcccug ggcguccuuc     3360

acccaggaca cguaccucgc ccuggagaac aucaucguca gcuucaagca gaaccugcgg     3420

gugaucaaca aggccaccaa ccgcuuccuc cacuacaacg aggagggcaa gaagaucuuc     3480

gugaagcagg gcaaggggga caacugggcc auccgcaagu ccaugcacaa ggacaccguc     3540

uucggcgagg ugaaccugcg gcgcaucaag accguggccc ugaacgaggc gaugaagaac     3600

ccccagagca ucgucgugaa ggacuucaag cggaagcucc uggagcugug gaaccucggg     3660

uucgacgcca agcgcaucaa gaaguacuuc gaggacaacc gggagacgug guccgacauc     3720

aaccugagca agaucgaggu guacuacuuc agcaaggaca ccaaggaccg cuucuucgcc     3780

acccggaagc cgcuggacac cuccuucgac cgcaagaaga ucgagaacaa caucacggac     3840

accggcaucc agaagauccu ccugcggcac cuggagcuca aggacaacaa ccccgacauc     3900

gccuucagcc ccgacgggau cgacgagaug aaccgcaaca ucauccagcu gaacaacggc     3960

aaguaccacc agcccaucau caagauccgg ugguacgagc aggcggacaa guucgccguc     4020

ggccagaccg ggaacaaguu cuccaaguuc guggaggccg ccaagggcac caaccuguuc     4080

uucgccgugu acgagagcaa cauccucgac aagaagacga acaccaucau caagaagcgc     4140

aacuacgcga ccaucccgcu gaacgucgcc aucgagcggc agaagcaggg gcugcccgug     4200

gcccccgagg acgagaacgg caacgacccc aucuucgugc ucuccccgaa cgaccugguc     4260

uaccugccca ccgacgacga gcucgccaac gggaucaucg cccagccccu ggaccgcggc     4320

cggaucuaca agauggugag cugcacgggc aacgaggggc acuucauccc cgcgcgcauc     4380

gccaacccga uccugcagac caucgagcuc ggcuccaaca acaaggccca gaaggccugg     4440

accgacgaga ugaucaagga gaucugcaug cccaucaagg uggaccggcu ggggaacguc     4500

cuggagagcu ccagcuccua caagaaguga                                      4530


<210>  2263
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2263
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugguc ccugaucgag       60

cacgacuuca agaacaagca ggggcagauc gagggccucg gggugcggau cauccccaug      120

agccaggaga uccugggcaa guucgacgcc ggccagucca ucagccagac cgccgaccgc      180

acgaaguacc gcgggguccg gcggcuguac cagcgcgaca accuccggcg cgagcggcug      240

caccgcgugc ugaagauccu cgacuuccug ccgaagcacu acuccgagag caucgacuuc      300

caggacaagg ugggccaguu caagcccaag caggagguca agcugaacua ccggaagaac      360

gagaagaaca agcacgaguu cguguucaug aacuccuuca ucgagauggu gagcgaguuc      420

aagaacgcgc agcccgagcu guucuacaac aaggggaacg gcgaggagac caagaucccc      480

uacgacugga cccuguacua ccugcgcaag aaggcccuca cccagcagau cacgaaggag      540

gagcuggccu ggcugauccu caacuucaac cagaagcggg gguacuacca gcugcgcggc      600

gaggacaucg acgaggacaa gaacaagaag uacaugcagc ugaaggucaa caaccucauc      660

gacuccggcg ccaaggugaa ggggaaggug cuguacaacg ucaucuucga caacggcugg      720

aaguacgaga agcagaucgu gaacaaggac gagugggagg ggcggaccaa ggaguucauc      780

aucaccacca agacgcugaa gaacggcaac aucaagcgca ccuacaaggc cguggacagc      840

gagaucgacu gggcggccau caaggccaag accgagcagg acaucaacaa ggccaacaag      900

accgucggcg aguacaucua cgagucccuc cuggacaacc cgagccagaa gauccggggg      960

aagcugguga agacgaucga gcgcaaguuc uacaaggagg aguucgagaa gcuccugucc     1020

aagcagaucg agcugcagcc cgagcuguuc aacgagagcc ucuacaaggc gugcaucaag     1080

gagcuguacc cccggaacga gaaccaccag uccaacaaca agaagcaggg cuucgaguac     1140

cucuucaccg aggacaucau cuucuaccag cgcccccuca agagccagaa guccaacauc     1200

agcgggugcc aguucgagca caagaucuac aagcagaaga acaagaagac cggcaagcug     1260

gagcucauca aggagccgau caagaccauc ucccggagcc acccccuguu ccaggaguuc     1320

cgcaucuggc aguggcugca gaaccucaag aucuacaaca aggagaagau cgagaacggg     1380

aagcuggagg acgugacgac ccagcugcuc cccaacaacg aggccuacgu cacccuguuc     1440

gacuuccuga acaccaagaa ggagcucgag cagaagcagu ucaucgagua cuucgugaag     1500

aagaagcuga ucgacaagaa ggagaaggag cacuuccgcu ggaacuucgu ggaggacaag     1560

aaguaccccu ucagcgagac gcgggcccag uuccuguccc gccucgccaa ggucaagggc     1620

aucaagaaca ccgaggacuu ccugaacaag aacacccagg ugggcagcaa ggagaacucc     1680

ccguucauca agcggaucga gcagcugugg cacaucaucu acagcguguc cgaccugaag     1740

gaguacgaga aggcccucga gaaguucgcg gagaagcaca accuggagaa ggacagcuuc     1800

cugaagaacu ucaagaaguu cccgcccuuc gucuccgacu acgccagcua cuccaagaag     1860

gccaucagca agcuccugcc caucaugcgc auggggaagu acugguccga gagcgccgug     1920

cccacccagg ugaaggagcg gucccugagc aucauggagc gcgucaaggu gcucccccug     1980

aaggagggcu acuccgacaa ggaccuggcc gaccuccuga gccgggucuc cgacgacgac     2040

auccccaagc agcugaucaa gagcuucauc uccuucaagg acaagaaccc ccucaagggg     2100

cugaacacgu accaggcgaa cuaccuggug uacggccgcc acagcgagac cggggacauc     2160

cagcacugga agaccccgga ggacaucgac cgguaccuca acaacuucaa gcagcacagc     2220

cugcgcaacc ccaucgugga gcaggucgug auggagaccc ugcggguggu ccgcgacauc     2280

ugggagcacu acggcaacaa cgagaaggac uucuucaagg agauccacgu ggagcucggc     2340

cgggagauga agucccccgc cgggaagcgc gagaagcuga gccagcggaa cacggagaac     2400

gagaacacca accaccgcau ccgggaggug cugaaggagc ucaugaacga cgccuccguc     2460

gagggcgggg ugcgcgacua cagccccucc cagcaggaga uccugaagcu guacgaggag     2520

ggcaucuacc agaacccgaa caccaacuac cucaaggugg acgaggacga gauccugaag     2580

auccggaaga agaacaaccc cacccagaag gagauccagc gcuacaagcu guggcucgag     2640

cagggguaca ucagccccua cacgggcaag aucauccccc ugaccaagcu guucacccac     2700

gaguaccaga ucgagcacau caucccgcag ucccgcuacu acgacaacag ccucggcaac     2760

aagaucaucu gcgaguccga ggucaacgag gacaaggaca acaagaccgc cuacgaguac     2820

cugaaggugg agaaggggag caucguguuc ggccacaagc ugcucaaccu ggacgaguac     2880

gaggcccacg ucaacaagua cuucaagaag aacaagacga agcugaagaa ccuccugucc     2940

gaggacaucc ccgagggguu caucaaccgg cagcugaacg acagccgcua caucuccaag     3000

cucgugaagg gccugcugag caacaucgug cgggagaacg gcgagcagga ggcgaccucc     3060

aagaaccuca uccccgucac cgggguggug accagcaagc ugaagcagga cuggggccug     3120

aacgacaagu ggaacgagau caucgccccg cgcuucaagc ggcucaacaa gcugaccaac     3180

uccaacgacu ucggguucug ggacaacgac aucaacgccu uccgcaucca gguccccgac     3240

agccugauca agggcuucag caagaagcgg aucgaccacc gccaccacgc ccucgacgcc     3300

cugguggugg cgugcacguc ccggaaccac acccacuacc ugagcgcccu caacgccgag     3360

aacaagaacu acucccugcg cgacaagcug gucaucaaga acgagaacgg ggacuacacc     3420

aagaccuucc agauccccug gcagggcuuc acgaucgagg ccaagaacaa ccucgagaag     3480

accgugguga gcuucaagaa gaaccugcgg gucaucaaca agaccaacaa caaguucugg     3540

uccuacaagg acgagaacgg caaccugaac cucgggaagg acggcaagcc caagaagaag     3600

cugcgcaagc agaccaaggg guacaacugg gcgauccgga agccgcugca caaggagacg     3660

gugagcggca ucuacaacau caacgccccc aagaacaaga ucgccaccuc cgugcgcacc     3720

cuccugaccg agaucaagaa cgagaagcac cuggccaaga ucacggaccu ccggauccgc     3780

gagaccaucc ugcccaacca ccugaagcac uaccucaaca acaaggggga ggccaacuuc     3840

agcgaggcgu ucucccaggg cggcaucgag gaccugaaca agaagaucac cacccugaac     3900

gaggggaaga agcaccagcc caucuaccgg gucaagaucu ucgagguggg cagcaaguuc     3960

uccaucagcg aggacgagaa cuccgccaag agcaagaagu acguggaggc cgccaagggg     4020

acgaaccucu ucuucgccau cuaccuggac gaggagaaca agaagcgcaa cuacgagacc     4080

aucccgcuga acgaggucau cacccaccag aagcaggucg cgggcuuccc caaguccgag     4140

cggcucagcg ugcagcccga cucccagaag gggaccuucc uguucacgcu gagccccaac     4200

gaccucgugu acgugccgaa caacgaggag cuggagaacc gcgaccuguu caaccucggc     4260

aaccugaacg uggagcagau cucccggauc uacaaguuca ccgacagcag cgacaagacc     4320

ugcaacuuca uccccuucca ggucuccaag cugaucuuca accucaagaa gaaggagcag     4380

aagaagcugg acguggacuu caucauccag aacgaguucg gccuggggag cccccagucc     4440

aagaaccaga aguccaucga cgacgugaug aucaaggaga agugcaucaa gcucaagauc     4500

gaccgccugg gcaacaucag caaggccuga                                      4530


<210>  2264
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2264
augaagacca uccugggccu cgaccugggg accaacagcg ugggcugggc cgucaucaac       60

uccgugauca aggagcaggc cgagcgcauc uggaucgaga uggcggggag ccggaucauc      120

cccauggacg ccgccgugcu gggcgacuuc gacaagggga acuccaucag ccagacgucc      180

gagcgcaccc ggcuccgcgg cguccggcgc cugcgggagc gccagcugcu ccggcgcgag      240

cggcugcaca aggugcugaa cauccucggc uuccugccga accacuaccu ggaguacauc      300

gacuucgaga agcaccccgg gaaguuccuc agcgacgccg agcccaagcu gcccugggug      360

aaggacgagc agggcaagua caccuuccug uuccaggccg cguucgacga gaugcucgcc      420

gacuucgcca uccaccagcc gucccugguc agcggggaca agaagauccc cuacgacugg      480

accaucuacu accugcgcaa gaaggcccuc acggagauga ucuccaagga ggagcuggcg      540

uggauccugc ucaacuucaa ccagaagcgg ggcuacuacc agcugcgcgg ggaggaggag      600

gaggacaacg ccggcaagag cguggaguuc uacgcccuga aggugcucac cgucgaggcc      660

accgacgaga agaagggccg ggacgugugg uacaacgugc accuggagaa cggguggauc      720

uaccgccggu ccagcuccgu cccccuggac ugggagggcc agaucaagga guucaucgug      780

accacggacc ucaaccccga cagcaccccg aagcuggaca aguacgggga ggugaagcgc      840

uccuuccggg cccccaagga ggacgacugg augcugauca agaagcgcac cgaggcggac      900

aucgccaaca cccacaagac ggucggcgcc uacaucuacg acacccuccu gcagaagccc      960

cagcagaaga ucaucggcaa gcuggugaag accguggagc ggaagcucua ccgcgacgag     1020

cugagccuga uccuccagaa gcagugcgcc uuccacgccg agcugcagga ccgcacccug     1080

uacgcgcggu gcgucgaggc ccucuacccc aacaacgagg ugcagcgccg gaacaucgcc     1140

aaccgcgacu ucguguaccu gcugauggac gacguccucu ucuaccagcg gccgcugaag     1200

acgaagaagu cccugaucgc caacugcccc uacgaggaga acgaguacgu ggaccgcgag     1260

accggggaga ucaagaaggc gccccucaag ugcaucgcca agagccaccc ccuguuccag     1320

gaguuccggc uguggaacuu ccucuccaac auccgcaucu accagaagga gaaggagauc     1380

aacggcaccc ugcaccugga cguggacguc accgccgagu uccugaagac ggaggacgac     1440

uacguggccc ucuucgacug gcugaacagc cggaaggaga ucgagcagaa gaccuuccug     1500

aaguacccga aguucgggcu caagaaggag aucagcaacu accgcuggaa cuacgucgag     1560

gacaagcagu accccugcaa cgagacccgg cacgccaucc ugucccgccu ggagaaggcc     1620

aacgugugca ucgacuuccu cagcaacgag aaggaggugg cgcuguggca cauccuguac     1680

uccgucagcg acaagcagga gaucgagaag gcccuccgga ccuucgccgc caagaacggc     1740

cuggacgagu ccuucgugga ggcguucaag aaguucccgc ccuucaagag cgaguacggg     1800

gccuacuccg ccaaggccau caagaagcug cucccccuga ugcgcugcgg caaguacugg     1860

gacgugagcu ccaucgacga cacgaccaag gagcggauca agaagaucgu cgccggcgag     1920

uacgacgagc gcaucgcggu gaaggugcgg gacaagguca ugaaccugag cgacaucucc     1980

ugcuucaagc gccucccccu guggcuggcc ugcuacaucg uguacaaccg gcacagcgag     2040

gacaaggagg ccgugaagug ggaguccccc gccgacaucg acgccuaccu cgcgagcuuc     2100

aagcagcacu cccugcgcaa ccccaucguc gagcagguca ucaccgagac ccugcgcgug     2160

gugcgggaca ucuggaagca ggucgggcgc aucgacgaga uccacaucga gcucggccgg     2220

gagaugaaga acccggccga caagcgcaag cggaugacgg cccagauccu ggagaacgag     2280

aacgccaacc ugcgcaucaa ggcccuccug gcggaguuca ugaaccccga guacgagauc     2340

gagaacgugc ggcccuacag ccccucccag caggagaucc ugcgcaucua cgaggacaug     2400

gugcuccacg ccgaggacca gcugccggag gagaucgacg ccauccugaa gaaguucaac     2460

agcuccaagg ugcccaccaa gagcgaguuc cuccgguaca agcuguggcu ggagcagaag     2520

uaccgcagcc ccuacaccgg ggagcucauc ccgcugggca agcuguucac cgccgcguac     2580

gagaucgagc acgugauccc ccagucccgg uacuucgacg acagcuucuc caacaagguc     2640

aucugcgaga gcgccgugaa caagcucaag gacaaccagc ugggguacga guucaucaag     2700

aaccaccacg gccagaaggu ggaggucggc uucgggaagu ccguggagau cuucagcgug     2760

gacuccuacg agcgcuucgu caaggagcag uacgccaaga gcgccgugaa gaugaagaag     2820

augcugaugg acgacauccc cgagcaguuc aucgagcggc agcucaacga cucccgcuac     2880

aucagcaagg ugaucaaggg ccugcugucc aacaucgucc gggagaagaa cgacagcggg     2940

gaguacgagc ccgaggccgu guccaagaac aucguggucu gcacgggcag cgugaccgac     3000

cgccucaagc gggacugggg gaugaacgac guguggaacu ccaucgucua cccgcgcuuc     3060

gagcggcuga acagccugac cgacacccag uccuucggcc acugggagaa caagagcggc     3120

aagaaggugu uccagacgga gcucccccug gaguaccaga agggguucag caagaagcgc     3180

aucgaccacc ggcaccacgc gauggacgcc aucgugaucg ccugcgccac ccgcaaccac     3240

gucaacuacc uguccaacga gagcgccucc cgcaacgcga agaucagccg guacgaccuc     3300

cagcgccugc ugugcgacaa gucccggacc gacgacaacg gcaacuacaa gugggugauc     3360

aagaagcccu gggacaccuu cacccaggac gcccgcgagg ugcucagcaa gaucgucgug     3420

ucccugaagc agaaccugcg gaucaucaac aagacgacca acguguaccg ccaccucgac     3480

gccaccggga accgggucua cgagaagcag aagaccggcg acagcugggc cauccgcaag     3540

cccaugcaca aggacacggu guucggcgcc gugaaccugc ggaaggucaa gguggugcgc     3600

cuguccgucg cgcucgacgc cccgaccaug aucguggaca agcgggucaa gaacaaggug     3660

uucgagcugc ugagcuacaa guacgacaag aagcgcaucg agaaguacuu caaggagaac     3720

gccgugcucu ggaaggagcu ggaccuggcc cgggucgcgg uguacuacuu caccgaggcc     3780

accacggagc cccucguggc cguccgcaag ccccuggacu ccaccuucaa cgagaagaag     3840

aucaaggaga gcgugaccga caccgggauc cagaagaucc ugcucaacca ccugugcacg     3900

aaggagggca agcccgaccu ggccuucucc gccgagggga ucgaggagau gaaccggaac     3960

cuccugcagc ugaacaacgg caagcgccac cagccgaucu acaaggugcg ggucuacgag     4020

ccccgcggga acaaguucag cgugggcgug cucggcaaca aggggaccaa gugggucgag     4080

gcggccaagg gcaccaaccu guucuucgcc aucuacauca ccgaggacgg gaagcggacg     4140

uacgagacca ucccccugaa cauggugauc gagcgcgaga agcagggccu cgcccccgug     4200

ccggacaaga acgagaaggg ggacaagcug cuguucuggc ucucccccaa cgaccugguc     4260

uaccugccca ccgaggagga gcucgaguuc ggccgcauca acgagcccau cgaccggggc     4320

cgcaucuaca agauggugag cuccagcggg aaccagugcu ucuucauccc gcaguccauc     4380

agcaacgcca ucauccccac caaggagcug ggcgcgaaca acaaggccga gcggagcugg     4440

gacggggcca ugaucaagaa caucugcauc cccgugaagg ucgaccgccu gggccggauc     4500

aucgagguga aguaccgcau caacgaguga                                      4530


<210>  2265
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2265
augaagacca uccugggccu cgaccugggg accaacagcg ugggcugggc cgucaucaac       60

uccgugauca aggagcaggc cgagcgcauc uggaucgaga uggcggggag ccggaucauc      120

cccauggacg ccgccgugcu gggcgacuuc gacaagggga acuccaucag ccagacgucc      180

gagcgcaccc ggcuccgcgg cgcccggcgc cugcgggagc gccagcugcu ccggcgcgag      240

cggcugcaca agguccugaa cauccucggc uuccugccga accacuaccu ggaguacauc      300

gacuucgaga agcaccccgg gaaguuccuc gccgacgcgg agcccaagcu ggccugggug      360

aaggacgagc agggcaagua caccuuccug uuccaggccg ccuucgacga gaugcucgcg      420

gacuucgcca uccaccagcc cagccuggug uccggggaca agaagauccc guacgacugg      480

accaucuacu accugcgcaa gaaggcccuc acggagauga ucagcaagga ggagcuggcc      540

uggauccugc ucaacuucaa ccagaagcgg ggcuacuacc agcugcgcgg ggaggaggag      600

gaggacaacg ccggcaaguc cgucgaguuc uacgcgcuga aggugcucac cguggaggcc      660

accgacgaga agaagggccg ggacgucugg uacaacgugc accuggagaa cggguggauc      720

uaccgccgga gcuccagcgu gccccuggac ugggagggcc aggucaagga guucaucgug      780

accacggacc ucaaccccga cgggaccccc aagcuggaca aguacggcga ggugaagcgc      840

uccuuccggg ccccgaagga ggacgacugg augcugauca agaagcgcac cgaggccgac      900

aucgccaaca cccacaagac ggucggcgcg uacaucuacg acacccuccu gcagaagccc      960

cagcagaaga ucaucgggaa gcuggugaag accguggagc ggaagcucua ccgcgacgag     1020

cugagccuga uccuccagaa gcagugcgcc uuccacgccg agcugcagga ccgcgcccug     1080

uacgcccggu gcgucgaggc gcucuacccc uccaacgagg ugcgccggaa gaacaucgcc     1140

aaccgcgacu ucguguaccu gcugauggac gacauccucu ucuaccagcg gccccugaag     1200

accaagaagu cccugaucgc caacugcccg uacgaggaga acgaguacgu cgaccgcgag     1260

acgggcgaga ucaagaagac gccccucaag ugcaucgcca agagccaccc ccuguuccag     1320

gaguuccggc uguggaacuu ccucagcaac auccgcaucu accagaagga gaaggagauc     1380

aacgggaccc ugcaccugga cguggacgug accgccgagu uccugaagac cgaggacgac     1440

uacgucgccc ucuucgacug gcugaacucc cggaaggaga ucgagcagaa gaccuuccug     1500

aaguacccca aguucggccu caagaaggag aucagcaacu accgcuggaa cuacguggag     1560

gacaagcagu acccgugcaa cgagacccgg cacgcgaucc ugagcucccu ggagaaggcc     1620

aacgucugca ucgacuuccu cagcaacgag aaggaggugg cccuguggca cauccuguac     1680

uccgugagcg acaagcagga gaucgagaag gcgcuccgca ccuucgccgc caagaacggg     1740

cuggacgagu ccuucgucga ggccuucaag aaguucccgc ccuucaagag cgaguacggc     1800

gccuacuccg cgaaggccau caagaagaug cugccccuca ugcggugcgg caaguacugg     1860

gacgugagcu ccaucgacga cacgaccaag gagcgcauca agaagaucgu gaccggggag     1920

uacgacgagc ggaucagcgu caaggugcgc gacaagguga ugaaccuguc cgacaucagc     1980

ugcuuccggc gccugccccu cuggcuggcc ugcuacaucg ucuacaaccg gcacuccgag     2040

gacaaggagg ccgugaagug ggagagcccc gccgacaucg acgcguaccu ggccuccuuc     2100

aagcagcaca gccuccgcaa ccccaucgug gagcagguca ucaccgagac gcugcgcgug     2160

gugcgggaca ucuggaagca ggucggccgc aucgacgaga uccacaucga gcuggggcgg     2220

gagaugaaga accccgccga caagcgcaag cggaugaccg cccagauccu cgagaacgag     2280

aacgccaacc ugcgcaucaa ggcgcugcuc gccgaguuca ugaacccgga guacgagauc     2340

gagaacgugc ggcccuacuc ccccagccag caggagaucc ugcgcaucua cgaggacacc     2400

gugcugcacg ccgaggacca gcuccccgag gagaucgacg ccauccugaa gaaguucaac     2460

uccagcaagg ucccgaccaa gagcgaguuc cugcgguaca agcucuggcu ggagcagaag     2520

uaccgcuccc ccuacacggg cgagcugauc ccccucggga agcuguucac cgcggccuac     2580

gagaucgagc acgugauccc gcagagccgg uacuucgacg acuccuucag caacaaggug     2640

aucugcgagu ccgccgucaa caagcugaag gacaaccagc ucggcuacga guucaucaag     2700

aaccaccacg gccagaaggu ggaggugggg uucggcaaga gcgucgagau cuucuccgug     2760

gacagcuacg agcgcuucgu gaaggagcag uacgccaagu ccgccgucaa gaugaagaag     2820

cugcugaugg acgacauccc cgagcaguuc aucgagcggc agcucaacga cagccgcuac     2880

aucuccaagg ugaucaaggg gcugcugagc aacaucgugc gggagaagaa cgacuccggc     2940

gaguacgagc ccgaggcggu cagcaagaac aucguggugu gcaccggguc cgucaccgac     3000

cgccucaagc gggacugggg caugaacgac guguggaaca gcaucgugua cccccgcuuc     3060

gagcggcuga acucccugac ggacacccag agcuucggcc acugggagaa caagagcggg     3120

aagaaggucu uccagaccga gcucccgcug gaguaccaga agggcuucuc caagaagcgc     3180

aucgaccacc ggcaccacgc cauggacgcc aucgugaucg ccugcgccac ccgcaaccac     3240

gugaacuacc ugagcaacga guccgcgagc cgcaacgcca agaucucccg guacgaccuc     3300

cagcgccugc ugugcgacaa gaaccggacc gacgacaacg ggaacuacaa gugggucauc     3360

aagaagcccu gggacacguu cacccaggac gcccgcgagg ugcucagcaa gaucgugguc     3420

ucccugaagc agaaccugcg gaucaucaac aagaccacca acguguaccg ccaccucgac     3480

gccacgggca accgggugua cgagaagcag aagaccggcg acagcugggc cauccgcaag     3540

cccaugcaca aggacaccgu cuucggggcg gugaaccugc ggaaggugaa ggucgugcgc     3600

cuguccgugg cccucgacac ccccgccaug aucgucgaca agcgggugaa gaacaagguc     3660

uucgagcugc ugagcuacaa guacgacaag aagcgcaucg agaaguacuu caaggagaac     3720

gccuuccucu ggaaggagcu ggaccuggcg aagguggccg uguacuacuu cacggaggcc     3780

accaccgagc cgcucgucgc cgugcggaag ccccuggacu ccaccuucaa cgagaagaag     3840

aucaaggaga gcgugacgga caccggcauc cagaagaucc ugcucaacca ccugugcacc     3900

aaggagggga agcccgaccu ggccuucucc gcggagggca ucgaggagau gaaccgcaac     3960

cuccugcagc ugaacaacgg caagagccac cagcccaucu acaagguccg gguguacgag     4020

ccgcgcggga acaaguucuc cgugggcguc cucgggaaca agggcaccaa guggguggag     4080

gccgccaagg ggacgaaccu guucuucgcc aucuacauca ccgaggacgg caagcggacc     4140

uacgagacca ucccccugaa cguggucauc gagcgcgaga agcaggggcu cgugcccgug     4200

cccgaccgga acaagaaggg cgacaagcug cuguucuggc ucagcccgaa cgaccugguc     4260

uaccugccca cggaggagga gcgcgaguuc ggccgcauca acgagcccau cgaccggggg     4320

cgcaucuaca agaugguguc cugcaccggc aacgaggggc acuucauccc cgccaacgug     4380

gcgaacccga ucguccccgu gaucgagcuc ggcagcaaca acaaggccca gcgggccugg     4440

accaacgaga ugaucaagga caucugcauc cccaucaagg uggaccgccu ggggcacauc     4500

aucgagguca aguaccggau cgacgaguga                                      4530


<210>  2266
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2266
augaagaaga uccugggccu cgaccugggg accaacagcg ugggcugggc cgucaucaac       60

uccgugguga acgacgaggg gaaggagcag cuggucaaca ucagcuccug cggcagccgc      120

aucaucccca uggacgccgc gacccucggg gacuucgaca agggcaacuc caagagcccg      180

guggccgagc ggacgcgccu gcggggcgug cgccggcugc ucgagcgcuc ccugcugcgg      240

cgcgagcggc uccaccgcgu ccugagcgug augggguucc ugcccgagca cuacgccgcc      300

cagcucgacc gguacggcaa guuccugccc gagaccgagc ccaagcuggc cugguacaag      360

gacgagaccg ggaaguaccg cuuccucuuc caggacuccu ucaacgagau gcuggaggac      420

uuccacgagc accacccgga gcugguggcg gagggcaaga agauccacua cgacuggacc      480

aucuacuacc uccggaagaa ggcccugagc cagaagauca cgaaggagga gcuggccugg      540

auccuccuga acuucaacca gaagcgcggg uacuaccagc ugcggggcga ggaggagcag      600

gaggacaaca agaaguccgu cgaguacuac gcccucaagg ugguggcggu cgaggacagc      660

ggcgaccgca aggggaagga caucugguac aacgugaagc uggagaacgg cuggguguac      720

cggcgcgccu ccgggguccc ccuggacugg gugggcaagg ugaaggaguu caucgucacc      780

accgaccucg acgagaaggg caaccccaag aaggacaagg acgggaacgu gaagcggagc      840

uuccgcgccc ccaaggagga cgacuggggc cugcugaaga cccggacgca ggcccagauc      900

gacgaguccg ggcagaccgu gggcaccuac aucuacgaga gccuccugcg caugccggac      960

cagaagaucc gcgggaagcu gguccggacc aucgagcgca aguacuacaa ggacgagcuc     1020

cggaagaucc ugaucaagca guccgaguuc cacacggccc ugcaggacca caaccuccug     1080

cuggcgugca ucgaggagcu cuaccccaac aacgaggccc accgccggcu gcugagcacc     1140

uccgacuuca ccuacuuccu cguggaggac auccuguucu accagcgccc ccucaagagc     1200

cagaagggcc agaucgacga cugccccuac gagagccacg uguaccggga caagaaggac     1260

ggcucccucc agcacguccc gcugaagugc gugagcaagu cccacccccu guuccaggag     1320

uuccgccucu ggcacuuccu gagcuuccug cggaucuacc agcgcgagcg gaugaucgac     1380

gggucccuga agcucgacgu cgacgugacc aacgaguucc ugcagaacga ggacgacuac     1440

gugaagcugu ucgacuggcu caacgagcgc aaggagaucg accagaagac gauccuggcc     1500

uacaagcccu ucggccugaa gaagaacgag cagagcaagu accgguggaa cuacgucgag     1560

gacaaguccu accccugcaa cagcacccgc gccgacauga ucuuccggcu cuccaaggcc     1620

ggggugccgg aggaguuccu gacccgcgag aaggaggagg cgcuguggca cauccucuac     1680

agcaucuccg acaagaagga gcugaccaag gcccugggca cguucgccga gaagaacggg     1740

cucaacgaga ccuucgugga gaucuucagc aagaugccgc ccuucgaguc cgacuacgcc     1800

gccuacagcc ugaaggcgau ccggaagcug cucgcccuga ugcgcauggg caaguacugg     1860

gacgagcagg ccaucgacga gcacacccgg ucccgcaucg agaagauccu gaccggcgag     1920

uacgacgaga cgauccgcac ccggguccgc gagaaggcca ugcaccucac cgacaucagc     1980

uccuuccggg ggcugccccu guggcucgcc ugcuacgugg uguacgaccg ccacagcgag     2040

gcgaaggagc uggucaagug ggagaccccc gacgacaucg accacuuccu gcggaaguuc     2100

aagcagcacu cccuccgcaa ccccaucgug gagcagauca ucauggagac gcugcggacc     2160

gugcgcgaca ucuggaagca ggagggcaag aucgacgaga uccacgucga gcuggggcgg     2220

gagaugaaga accccgccaa ggagcgcgcc cggaucaccg cccaggugcu cgagaacgag     2280

aacaccaacc ugcgcaucaa ggcgcugcuc gccgaguuca ugaaccccga guucgagauc     2340

gagaacgugc ggccguacag ccccgcccag caggagaucc ugcgcaucua cgaggagggc     2400

guccugagcg ggguggccga gaaggaccuc cccgacgaca ucgccuccau ccugaagaag     2460

uuccgggaga acgacgugaa gaagcgcccc acgaccagcg agguccugcg guacaagcuc     2520

uggcuggagc agcgcuaccg guccccguac accggccgca ucaucccccu gggcaagcug     2580

uucacccccg cguacgagau cgagcacgug aucccgcaga gccgguacuu cgacgacucc     2640

uucacgaaca aggugaucug cgagagcgcc gucaacaagc ugaaggacaa cugccugggg     2700

uacgaguucg ugaagaagca cuccggcgag aagguggagc ucgggaacgg cgagaccgcc     2760

accaucuuca gcgucgagga guacgagcgc uucgugaagg acuccuacuu caacaacagc     2820

aagaagauca agaagcugcu gcucgaggac auccccgaca acuucaucga gcgccagcug     2880

aacgacuccc gguacaucag caaggugguc aagucccugc ucagcaacau cgugugcgag     2940

gagggcgagc aggaggggau guccaagaac gugaucgucu gcaccggcgg ggugaccgac     3000

cgccugaaga aggacugggg cgugcaggac gucuggaacc ggaucauccu gccccgcuuc     3060

cuccggcuga acaagaugac ggaccgcacc gacuucaccg ccgugagcac caacgggcac     3120

cuccugcccg ccgugccgcu gcaccuccag aagggcuuca acaagaagcg gaucgaccac     3180

cgccaccacg cgauggacgc caucgucauc gccugcgccg gccggaacau cgugaacuac     3240

cugaacaacg ccuccgcgcg caaggggagc gagaucagcc gguacgaccu gcagcaccuc     3300

cugugcgaca aggugaagac ggacgccaac ggcaacuaca aguggauccu gcgcaagccc     3360

ugggagaccu ucacccagga ccuguacgcc gcccucacca acaucgucgu guccuucaag     3420

cagaaccugc gggugaucaa ccgcacgacc aaccacuacc agcacuacaa cgaggacggg     3480

gagaagaaga ugauccccca gaccaagggc gaccggcugg cgauccgcaa gcccaugcac     3540

aaggacaccg ucuacgggga ggugaaccuc cggaaggaga agacgcugcc gcugaaggac     3600

guggucaaga accccagcgc cgugguggac aagucccuca agaacaagcu guacgagcug     3660

cucaagagcc aguacgaccu gaaggccauc accaaguacu ucgagaccca ccaggacguc     3720

ugggccgacg ugaaccugaa gaagaucaag guguacuacu ucaccaagga gacgaacgag     3780

cgcuucuucg ccacccggaa gucccucgac agcuccuuca gccagaagaa gaucgaggag     3840

gaggucaccg acaccggcau ccagaagauc cugcugcgcc accuccagca gaacggcaac     3900

aaccccgaca ucgcguucuc ccccgacggg guggagcgca ugaaccagaa caugacgauc     3960

cugaacgacg gcaaguggca ccagccgauc uacaaggucc ggaccuacga gaaggccgac     4020

aaguucgccg ugggggagaa gggcaacaag gugaagaagu ucgucgaggc cgccaagggg     4080

accaaccugu ucuucgcggu guacgagagc auccaggagg acgaggccac cggcaagcag     4140

guccgcaagc ggacguucgc caccaucccc cucaacgagg ugaucaagcg caagaagcag     4200

ggccugcccg ccgcccccga ggacuccaag gggaaccugc cgaucuucgu gcucagcccc     4260

aacgaccugg uguaccugcc caccgaggag gagcggaacu ccggcaagau cauccagccc     4320

cucgaccgcg agcggaucua caagaugguc agcugcaccg agggggagug ccacugcauc     4380

ccggaguacg uggcgaaccc cauccugcag acgaucgagc ugggcuccaa caacaaggcc     4440

cagcgcgccu ggagcgggga gaugaucaag gagaucugcg ugcccgucaa gguggaccgg     4500

cucggcaacg ugucccugau caagaucuga                                      4530


<210>  2267
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2267
augaagacca uccugggccu cgaccugggg accaacagcg ugggcugggc cgucaucaac       60

uccgugauca aggagcaggc cgagcgcauc uggaucgaga cggcggggag ccggaucauc      120

cccauggacg ccgccgugcu gggcgacuuc gacaagggga acuccaucag ccagaccucc      180

gagcgcaccc ggcuccgcgg cgcccggcgc cugcgggagc gccagcugcu ccggcgcgag      240

cggcugcaca agguccugaa cauccucggc uuccugccga accacuaccu ggaguacauc      300

gacuucgaga agcaccccgg gaaguuccuc gccgacgcgg agcccaagcu ggccugggug      360

aaggacgagc agggcaagua caccuuccug uuccaggccg ccuucgacga gaugcucgcg      420

gacuucgcca uccaccagcc cagccuggug uccggggaca agaagauccc guacgacugg      480

acgaucuacu accugcgcaa gaaggcccuc accgagauga ucagcaagga ggagcuggcc      540

uggauccugc ucaacuucaa ccagaagcgg ggcuacuacc agcugcgcgg ggaggaggag      600

gaggacaacg ccggcaaguc cgucgaguuc uacgcgcuga aggugcucac cguggaggcc      660

accgacgaga agaagggccg ggacgucugg uacaacgugc accuggagaa cggguggauc      720

uaccgccgga gcuccagcgu gccccuggac ugggagggcc aggucaagga guucaucgug      780

acgaccgacc ucaaccccga cgggaccccc aagcuggaca aguacggcga ggugaagcgc      840

uccuuccggg ccccgaagga ggacgacugg augcugauca agaagcgcac cgaggccgac      900

aucgccaaca cgcacaagac cgucggcgcg uacaucuacg acacccuccu gcagaagccc      960

cagcagaaga ucaucgggaa gcuggugaag accguggagc ggaagcucua ccgcgacgag     1020

cugagccuga uccuccagaa gcagugcgcc uuccacgccg agcugcagga ccgcgcccug     1080

uacgcccggu gcgucgaggc gcucuacccc uccaacgagg ugcgccggaa gaacaucgcc     1140

aaccgcgacu ucguguaccu gcugauggac gacauccucu ucuaccagcg gccccugaag     1200

acgaagaagu cccugaucgc caacugcccg uacgaggaga acgaguacgu cgaccgcgag     1260

accggcgaga ucaagaaggc gccccucaag ugcaucgcca agagccaccc ccuguuccag     1320

gaguuccggc uguggaacuu ccucagcaac auccgcaucu accagaagga gaaggagauc     1380

aacgggaccc ugcaccugga cguggacgug accgcggagu uccugaagac ggaggacgac     1440

uacgucgccc ucuucgacug gcugaacucc cggaaggaga ucgagcagaa gaccuuccug     1500

aaguacccca aguucggccu caagaaggag aucagcaacu accgcuggaa cuacguggag     1560

gacaagcagu acccgugcaa cgagacccgg cacgccaucc ugagcucccu ggagaaggcc     1620

aacgucugca ucgacuuccu cagcaacgag aaggaggugg cgcuguggca cauccuguac     1680

uccgugagcg acaagcagga gaucgagaag gcccuccgca ccuucgccgc caagaacggg     1740

cuggacgagu ccuucgucga ggccuucaag aaguucccgc ccuucaagag cgaguacggc     1800

gcguacuccg ccaaggccau caagaagaug cugccccuca ugcggugcgg caaguacugg     1860

gacgugagcu ccaucgacga cacgaccaag gagcgcauca agaagaucgu gaccggggag     1920

uacgacgagc ggaucgccgu caaggugcgc gacaagguga ugaaccugag cgacaucucc     1980

ugcuuccggc gccugccccu cuggcuggcc ugcuacaucg ucuacaaccg gcacagcgag     2040

gacaaggagg cggugaagug ggaguccccc gccgacaucg acgccuaccu ggccagcuuc     2100

aagcagcacu cccuccgcaa ccccaucgug gagcagguca ucaccgagac gcugcgcgug     2160

gugcgggaca ucuggaagca ggucggccgc aucgacgaga uccacaucga gcuggggcgg     2220

gagaugaaga accccgccga caagcgcaag cggaugaccg cgcagauccu cgagaacgag     2280

aacgccaacc ugcgcaucaa ggcccugcuc gccgaguuca ugaacccgga guacgagauc     2340

gagaacgugc ggcccuacag ccccucccag caggagaucc ugcgcaucua cgaggacacc     2400

gugcugcacg cggaggacca gcuccccgag gagaucgacg ccauccugaa gaaguucaac     2460

agcuccaagg ucccgaccaa gagcgaguuc cugcgguaca agcucuggcu ggagcagaag     2520

uaccgcagcc ccuacacggg cgagcugauc ccccucggga agcuguucac cgccgccuac     2580

gagaucgagc acgugauccc gcagucccgg uacuucgacg acagcuucuc caacaaggug     2640

aucugcgaga gcgccgucaa caagcugaag gacaaccagc ucggcuacga guucaucaag     2700

aaccaccacg gccagaaggu ggaggugggg uucggcaagu ccgucgagau cuucagcgug     2760

gacuccuacg agcgcuucgu gaaggagcag uacgccaaga gcgccgucaa gaugaagaag     2820

cugcugaugg acgacauccc cgagcaguuc aucgagcggc agcucaacga cucccgcuac     2880

aucagcaagg ugaucaaggg gcugcugucc aacaucgugc gggagaagaa cgacagcggc     2940

gaguacgagc ccgaggccgu cuccaagaac aucguggugu gcaccgggag cgucaccgac     3000

cgccucaagc gggacugggg caugaacgac guguggaacu ccaucgugua cccccgcuuc     3060

gagcggcuga acagccugac ggacacccag uccuucggcc acugggagaa caagagcggg     3120

aagaaggucu uccagaccga gcucccgcug gaguaccaga agggcuucag caagaagcgc     3180

aucgaccacc ggcaccacgc cauggacgcc aucgugaucg cgugcgccac ccgcaaccac     3240

gugaacuacc uguccaacga gagcgccucc cgcaacgcca agaucagccg guacgaccuc     3300

cagcgccugc ugugcgacaa gaaccggacc gacgacaacg ggaacuacaa gugggucauc     3360

aagaagcccu gggacacguu cacccaggac gcccgcgagg ugcucuccaa gaucgugguc     3420

agccugaagc agaaccugcg gaucaucaac aagaccacca acguguaccg ccacuucgac     3480

gcgacgguga accgggucua cgagaagcag aagaccggcg acuccugggc cauccgcaag     3540

cccaugcaca aggacaccgu guucggcgcc gugaaccucc ggaaggucaa gguggugcgc     3600

cugagcgucg cccuggacac ccccgcgaug aucguggaca agcgggucaa gaacaaggug     3660

uucgagcucc uguccuacaa guacgacaag aagcgcaucg agaaguacuu caaggagaac     3720

gccuuccugu ggaaggagcu cgaccuggcc aagguggccg ucuacuacuu cacggaggcc     3780

accaccgagc cgcugguggc ggugcggaag ccccucgaca gcaccuucaa cgagaagaag     3840

aucaaggagu ccgucacgga caccgggauc cagaagaucc ugcugaacca ccucugcacc     3900

aaggagggca agcccgaccu ggccuucagc gccgagggga ucgaggagau gaaccgcaac     3960

cugcuccagc ugaacaacgg caagucccac cagcccaucu acaaggugcg gguguacgag     4020

ccgcgcggga acaaguucag cgucggcgug cugggcaaca aggggaccaa guggguggag     4080

gccgccaagg gcacgaaccu cuucuucgcg aucuacauca ccgaggacgg gaagcggacc     4140

uacgagacca ucccccugaa cgucgugauc gagcgcgaga agcagggccu ggugcccguc     4200

cccgaccgga acgagaaggg ggacaagcuc cuguucuggc uguccccgaa cgaccucgug     4260

uaccugccca cggaggagga gcgcgaguuc ggccgcauca acgagcccau cgaccggggc     4320

cgcaucuaca agauggugag cugcaccggg aacgagggcc acuucauccc cgccaacguc     4380

gccaacccga ucgugcccgu gaucgagcug ggguccaaca acaaggccca gcgggccugg     4440

accaacgaga ugaucaagga caucugcauc cccaucaagg ucgaccgccu cggccacauc     4500

aucgagguga aguaccggau cgacgaguga                                      4530


<210>  2268
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2268
augaagaaga cccugggccu cgaccugggg accaacagca ucggcugggc cgugaucaac       60

uccaacaucg acagcgaggg gaaggagaag cuggucggca ucuccagcug cgggucccgc      120

aucaucccca uggacgccac gacccucggc gacuucggca aggggaacac caagagcccg      180

guggcggagc ggacccgccu gcggggcauc cgccggcugc ucgagcgcuc ccugcugcgg      240

cgcgagcggc uccaccgcgu gcugagcguc augggguucc ugcccgagca cuacgccucc      300

cagcucgacc gguacggcaa guuccugccc gagacggagc ccaagcuggc cugguacaag      360

gacgacagcg ggcgcuacca guuccucuuc cagaaguccu uccacgagau gcuggaggac      420

uuccggcagc accagccgga gcugguggcc ggcgagaaga agauccccua cgacuggacc      480

aucuacuacc uccgcaagaa ggcccugagc caggagauca ccaaggagga gcuggcgugg      540

auccuccuga acuucaacca gaagcggggc uacuaccagc ugcgcgggga ggaggagcag      600

gaggagaaca acaaguccgu ggaguaccac gcccucaagg ucgugagcgu ggaggacucc      660

ggcgagcgga aggggaagga caucugguac aacgucaccc uggagaacgg cuggguguac      720

cgccgggcca gcaacauccc ccuggacugg acgggcaagg ugaaggaguu cgucgugacc      780

accgagcucg acgacgccgg gaaccccaag aaggacaagg agggcaacgu gaagcgcucc      840

uuccgggcgc cgaaggagga cgacuggggg cugcugaaga cccgcacgca ggcccagauc      900

gacgagagcg gcaagaccgu cgggaccuac aucuacgagu cccuccugug caugcccaac      960

cagaagaucc gcggcaagcu ggugcggacc aucgagcgca aguacuacaa ggacgagcuc     1020

cggcagaucc uggugaagca gagcgaguuc cacgccgccc ugcaggacca caaccuccug     1080

cugagcugca ucgaggagcu cuaccccaac aacgaggccc accgccggcu gcuguccgcg     1140

agcuccuuca ucuacuuccu caucgaggac auccuguucu accagcgccc ccucaagagc     1200

cagaagggcc ucaucgacaa cugcccguac gagucccaca ucuacaagga caagaaggac     1260

gggagccugc accacguccc ccugaagugc guguccaaga gccacccccu cuuccaggag     1320

uuccggcugu ggcaguuccu guccaaccug cgcaucuacc agcgggagcg caugauggac     1380

ggcagccuca agcuggacgu ggacgucacg cgggaguucc ugcccuccga ggaggacuac     1440

gugaagcugu ucgacuggcu gaacgagcgc aaggagauca gccagaaguu ccugcucgcc     1500

uacaagccgu ucgggcugaa gaagaacgag caggccaacu accgguggaa cuacguggag     1560

gacaaguccu accccugcaa cgagacccgc gccgagauca agagccggcu guccaaggcc     1620

ggcguccccg aggaguuccu caccgaggag aaggaggagg cgcuguggca cauccuguac     1680

agcaucuccg acaagaagga gcucaccaag gcccugggga cguucgccgc caagaacugc     1740

cugaacgaga gcuucgugga ggucuucgcc aagaucccgc ccuucgaguc caacuacgcg     1800

gccuacagcc ucaaggccau ccgcaagcug cuggcccuca ugcggauggg caaguacugg     1860

aacgagcagg ccaucgaccg ccagacccgg gaccgcaucg agaagauccu gaccggcgag     1920

uacgacgaga ccauccgcag ccgggugcgc gagaaggcga ugcugcucac ggacaucucc     1980

agcuuccggg ggcugccccu guggcucgcc ugcuacaucg uguacgaccg ccacuccgag     2040

agcaaggagc uggucaagug ggagaccccc gccgacaucg accacuuccu guccaaguuc     2100

aagcagaaca gccuccggaa ccccaucgug gagcagguca ucaccgaguc ccugcgcacc     2160

gugcgggaca ucuggaagca ggagggcaag aucgacgaga uccacgugga gcuggggcgc     2220

gagaugaaga acccggccaa ggagcgggcg cgcaucacgg cccaggugca ggagaacgag     2280

aacaccaacc uccggaucaa ggcccugcug gccgaguuca ugaaccccga guucgagauc     2340

gagaacgucc accccuacag ccccggccag caggagaucc uccgcaucua cgaggacggg     2400

gugcuguccg gcaucgccga gaaggaccug ccggacgaca ucaccgcgau ccucaagaag     2460

uuccgggaga acgacgugaa gaagcgcccc accacgagcg agguccugcg guacaagcug     2520

uggcucgagc agcgcuaccg gucccccuac accggccgcg ugaucccgcu ggggaagcug     2580

uucacccccg ccuacgagau cgagcacgug aucccccaga gccgguacuu cgacgacucc     2640

aucagcaaca aggucaucug cgaguccgcc gugaacaagc ucaaggacaa cugccugggc     2700

uacgaguuca ucaagaagca cagcggggag augguggagc ugggcaacgg cgagaccguc     2760

cccguguucu ccguggagga guacgagcgc uucgucaagg agagcuacuu cgggaacagc     2820

aagaagauga agaagcuccu gcuggaggac aucccggacu ccuucaucga gcggcagcuc     2880

aacgacagcc gcuacaucuc ccgcguggug acgagccugc uguccaaccu cgucugcgag     2940

gagggcgagc aggacgggcu gagcaagaac gugaucgugu gcaccggcgg gaucaccgac     3000

cggcugaaga aggacugggg cguccaggag guguggaacc gcaucauccu cccccgguuc     3060

cugcgccuga acgagaucac cggccggacc gacuucacgu ccaccagcgu gaacgggcac     3120

cuccugcccg cccugccccu cuaccugcag aagggcuuca acaagaagcg caucgaccac     3180

cggcaccacg ccauggacgc gaucgucauc gccugcgcca accgcaacau cgugaacuac     3240

cugaacaacu ccagcgcccg gaagaacucc gagaucagcc gcuacgaccu ccagcggcug     3300

cugugcgaga aggugaagac cgacgccaac gggaacuaca aguggauccu ccgcaagccg     3360

ugggagaccu ucacgcagga cgucuacgcg gcccugacca acaucguggu guccuucaag     3420

cagaaccugc gggucaucaa ccgcaccacc aacuacuacc agcacuacaa cgaggccggc     3480

gagaagcgga ugauccccca gacgaagggg gaccgcuggg ccauccggaa gcccaugcac     3540

aaggacaccg uguacggcga ggugaaccuc cgcaaggaga agacccugcc ccugcgggac     3600

gucgugaaga acccgagcau cguggucgac aagucccuca agaacaagcu guacgagcug     3660

cucaagagcc aguacgaccu gaaggcgauc gccaaguacu ucgagaccca ccaggacgug     3720

ugggccgacg ugaaccugaa gaagaucaag gucuacuacu ucacgaagga gaccaacgag     3780

cgcuucuucg ccacccgcaa gucccucgac cccagcuucg accagaagaa gaucgaggag     3840

gaggugaccg acacgggcgu gcagaagauc cugcugcacc accuccagca gaacaacaac     3900

gaccccgaca uggccuucuc ccccgacggg aucgaccgga ugaaccagaa caugaccauc     3960

cugaacgacg gcaaguggca ccagccgauc uacaaggucc gcaccuacga gaaggcggac     4020

aaguucgccg ugggggagag cggcaacaag gccaagaagu ucguggaggc cgccaagggg     4080

accaaccugu ucuucgcggu cuacgagagc gugcaggagg acgaggccau cggcaagcag     4140

gucugcaagc ggacguucgc caccaucccc cucaacgagg ugaucaagcg caagaagcag     4200

ggccugcccg ccgcccccga ggaccugaac gggaaccucc cgaaguucgu gcuguccccc     4260

aacgaccugg ucuaccuccc caccgaggag gagcggaaca gcucccgcau cauccagccc     4320

cuggaccggg agcgcaucua caagauggug agcuccagcg gcucccagug cuucuucauc     4380

aagguguucg ucgcgaacag caucugggac aagaacgagu acuccagccu gaacaagaug     4440

gagcgggcca ucaccaacga gaugaucaag gagaucugcg ugccgaucaa gaucgaccgc     4500

cucgggaacg ugucccugau ccagaucuga                                      4530


<210>  2269
<211>  4533
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2269
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccugguggac       60

uccgaggagc agcggauccu cgggaugggc agccgcauca uccccaugga ccaggggguc      120

cuggacaccu ucuccggcgg caacccggug gagacgcaga ccgccgcgcg gaccguguac      180

cgcgggaccc ggcgccugcg ggagcgcgcc cuccugcggc gcgagcggcu gauccgcguc      240

cucaacauga ugaaguuccu gcccgagcac uacgccagcc agaucgacuu cgagaagcgg      300

cugggccagu ucuucgacga gaaggagccc aagcucgccu ggaagcagaa cgacgagggg      360

cgcuucgagu ucaucuucca gcaguccuuc aacgagauga ucgaggaguu caagaacaag      420

ggcguggugc gggaggacaa gaagaucccc uacgacugga cgaucuacua ccugcgcaag      480

aaggcccuga gccaggagau cgagaaggag gagcucgcgu ggauccugcu gaacuucaac      540

cagaagcggg gguacuacca gcuccgcggc gaggaggagg aggagaaccc gaacaagcug      600

gucgaguacu acucccugaa gaucguggac gugaucgccg acgagaagcc caacagcaag      660

uccgacaucu gguacagccu ccaccuggag aacggcugga ucuaccggcg cuccagcaag      720

aucccccugu ucggguggaa ggacaagacc cgggacuuca ucgucaccac cgaccucaac      780

gacgacggca cggugaagaa ggacaaggag gggaucgaga agcgcuccuu ccgcgccccc      840

agcgagaacg acugggugcu gcugaagaag aagaccgagu ccgacaucga gaagucccac      900

aagaccgucg gcgccuacgu guacgagacc cuccugcaga acccgaugca gaaggugcgg      960

ggcaagcugg uccgcacgau cgagcggaag uucuacaagg acgagcucaa gcagauccug     1020

gagaagcagc gcgaguucca ccccgagcug cgggacgagg accucuacaa cgacugcgug     1080

cgcgagcugu accggagcaa cgagaccuac caguucaucc ugagcaagcg cgacuucgug     1140

caccucuucc uggacgacau ccuguucuac cagcggcccc uccgcuccca gaaguccgcg     1200

aucggggacu gcauccugga guucaagacc uacaaggaca aggacggcaa caacaucaag     1260

gaguaccuga aggucaucag caagagcaac ccccucuacc aggaguuccg gaucuggcag     1320

uggcuguaca accugaagau cuaccgcaag gaggacgacg aggacgugac ccuccaguuc     1380

aucgccaaca ucgaggacaa ggagcagcug uucgacuucc ugagcaaccg gaaguccauc     1440

gagcagaagc cgcuccugga guaccugauc aaggccaagg ggcugaagaa gcaggucaag     1500

acggaggccu accgcuggaa cuacguggag gacaagaucu accccugcag cgagaccaag     1560

accaugaucu ccacccggcu cgccaaggug gagaacaucc ccgacaacuu cuucacgaag     1620

gagaucgagc agaagcugug gcacaucauc uacagcguga ccgacaagaa cgaguucgag     1680

aaggcgcugc gcaccuucgc caagaaguac gaccucgaca ucgccuccuu cgucgacaac     1740

uucaagaaga ucccgccguu caagaacgag uacggcagcu ucuccgagaa ggccaucaag     1800

aagcugcugc cccucaugcg gcuggggaag uacuggaagu ggaacgacau cgacaacaac     1860

acccagaacc gcaucagcaa gaucaucacg ggcgaguacg acgaggagau caaggacauc     1920

gugcgggaga aguccaucgc ccugaccaac gagaacgacu uccagggccu ccagcugugg     1980

cuggcgcagu accucgucua cgggcgccac agcgaggccg acaucgccgg caaguggcac     2040

uccguggccg accuggagaa guaccugaac gaguucaagc agcacagccu ccgcaacccc     2100

aucguggagc agaucaucac cgagacccug cgggucgugc gcgacaucug gcggaaguac     2160

gggcagggcg ccgagaacuu cuucuccgag auccacgugg agcuggggcg cgacaugaag     2220

aacacggcgg acgagcggaa gaagaucguc aacgugguga ccgagaacga gaacaccaac     2280

cuccgcauca aggcccugcu gauggagcuc aagaacaaca gcgacggcaa gcuggagguc     2340

gagaacgugc ggcccuacuc cccgacccag caggacaucc ugaagaucua cgaggaguac     2400

gccaucagca cgggccucga caacgagaag gacgagaagg ugaaggagga caucaagaag     2460

aucucccgcg ucgcccagcc caccaccacc gagcugcagc gguacaagcu guggcucgag     2520

cagaaguacu gcagccccua cacggggaag gugauccccc ugggcaagcu guucaccgag     2580

gaguaccaga ucgagcacau cauccccaag agccgcuacu acgacgacuc cuucagcaac     2640

aaggugaucu gcgaggccgc ggucaacaag cucaaggaca agugccuggg gcuggaguuc     2700

aucaagaacu accacggcca gaucguggag accggguucg gccagaaggu gaccaucuuc     2760

gacgaggagg ccuaccagaa cuucgucaag cagcacuacg ccuacaacca cuccaagcgg     2820

accaagcucc ugcuggagga gauccccgag aagaugaucg agcgccagau gaacgacacg     2880

cgguacauca gcaaguucgu gcucccccug cuguccaacc ucgugcgcgc caaggagaac     2940

gacaacggcg ucaacagcaa gaacgugcug cccgugaacg ggaagaucac caccaugcug     3000

aagcaggacu ggggccucaa cgacgucugg aacgaccuga uccugccgcg guucgugcgc     3060

augaacgagc ucgcgaagac caucgccuuc acguccugga acgagcagca ccagaaguac     3120

cugcccaccg ugccccugga gcucagcaag ggguuccaga agaagcggau cgaccaccgc     3180

caccacgcca uggacgcccu ggucaucgcc ugcgcgaccc gggaccacgu gaaccugcuc     3240

aacaacaagc acgccaacac cgacacgauc cgcuacgacc ugcagcggaa gcugcgccuc     3300

uucgagcgcg ugaccuacau cgacccgcag accaagaaca acgucaccaa ggacaucccc     3360

aaggaguuca agaagcccug ggacaacuuc acgguggacg cccggaacga gcuggagaag     3420

aucaucgugu ccuucaagca gaaccugcgc aucaucaaca aggccaccaa caucuacacc     3480

aaguacgaga acggcaagaa gaucaagguc ggccagaagg ggcucaacug ggccauccgg     3540

aagccccugc acaaggagac cguguucgcg aaggugagcc ugcgcaagcg gaagacgguc     3600

cgccucuccg aggcccugaa ggacuggaag aagaucgugg acaagaagcu gaagcaggag     3660

aucaagcggc ugaccugcca guacggcaag uucgacgugg acaccauccu gcgcuacuuc     3720

aaggaccgga aguaccaguu cggggagguc gacgugagca aggugaagau guacuacuuc     3780

gacgaggaga acgccgccgu ccgcaagaac guggacaccu ccuucacgga gaaguucauc     3840

cagggcagcg ugaccgacac cgggauccag aagauccugc ucaaccaccu ggaggccaag     3900

ggcaacaagg ucgagaucgc guucuccccg gagggcaucg aggagaugaa caagaacauc     3960

auccagcuga acgaggggaa gcuccaccuc cccaucuuca aggugcgggu guacgagacc     4020

aucggcaaca aguucagcgu cggggugaag ggcaacaaga aggacaagua cguggaggcc     4080

gccaagggga cgaaccuguu cuucgccauc uacgucgacg agaacggcau ccgcuccuac     4140

gagaccaucc cccucaacau cgugaucgag cggcugaagc agggccugag cgucgugccc     4200

gagaagaacc gcaaggagca cagccuccug uucuaccugu ccccgaacga ccucguguac     4260

guccccaucg aggacgagcg ggagaacauc cacgcgguga accuggacca gcugaacaac     4320

gagcagcgca agcggaucua caagauggug agcugcaccg gguccgagug ccacuucguc     4380

cccuacuacg uggccagccc caucgugaac aaggucgagu acuccagccu caacaagauc     4440

ggccgcuccc ugaccgggga gaugaucaag gacgugugca ucaagcugaa gguggaccgg     4500

cucggcaaca ucaaggggau cagcggccug uga                                  4533


<210>  2270
<211>  4533
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2270
augaagcgca uccugggccu cgaccugggg accaacagca ucggcugggc ccugguggac       60

uccgaggagc agcggauccu cgggaugggc agccgcauca uccccaugga ccaggggguc      120

cuggacaccu ucuccggcgg caacccggug gagacgcaga ccgccgcgcg gaccgccuac      180

cgcgggaccc ggcgccugcg ggagcgcgcc cuccugcggc gcgagcggcu gauccgcgug      240

cucaacauga ugaaguuccu gcccgagcac uacgccagcc agaucgacuu cgagaagcgg      300

cugggccagu ucuucgacga gaaggagccc aagcucgccu ggaagcagaa cgacgagggg      360

cgcuucgagu ucaucuucca gcaguccuuc aacgagauga ucgaggaguu caagaacaag      420

ggcgucgugc gggaggacaa gaagaucccc uacgacugga cgaucuacua ccugcgcaag      480

aaggcgcuga gccaggagau cgagaaggag gagcucgccu ggauccugcu gaacuucaac      540

cagaagcggg gguacuacca gcuccgcggc gaggaggagg aggagaaccc gaacaagcug      600

guggaguacu acucccugaa gaucgucgac gugaucgccg acgagaagcc caacagcaag      660

ggcgacaucu gguacucccu ccaccuggag aacgggugga ucuaccggcg cagcuccaag      720

aucccccugu ucggcuggaa ggacaagacc cgggacuuca ucgugaccac cgaccucaac      780

gacgacggga cggucaagaa ggacaaggag ggcaucgaga agcgcagcuu ccgcgccccc      840

uccgagaacg acugggugcu gcugaagaag aagaccgaga gcgacaucga gaagucccac      900

aagaccgugg gcgcguacgu cuacgagacc cuccugcaga acccgaugca gaaggugcgg      960

gggaagcugg ugcgcacgau cgagcggaag uucuacaagg acgagcucaa gcagauccug     1020

gagaagcagc gcgaguucca ccccgagcug cgggacgagg accucuacaa cgacugcguc     1080

cgcgagcugu accggagcaa cgagaccuac caguucaucc uguccaagcg cgacuucgug     1140

caccucuucc uggacgacau ccuguucuac cagcggcccc uccgcagcca gaaguccgcc     1200

aucggcgacu gcauccugga guucaagacc uacaaggaca aggacgggaa caacaucaag     1260

gaguaccuga aggugaucag caagagcaac ccccucuacc aggaguuccg gaucuggcag     1320

uggcuguaca accugaagau cuaccgcaag gaggacgacg aggacgucac ccuccaguuc     1380

aucgccaaca ucgaggacaa ggagcagcug uucgacuucc uguccaaccg caagagcauc     1440

gagcagaagc cgcuccugga guaccugauc aaggccaagg gccugaagaa gcaggucaag     1500

acggaggccu accgguggaa cuacguggag gacaagaucu accccugcuc cgagaccaag     1560

accaugauca gcacccggcu cgcgaaggug gagaacaucc ccgacaacuu cuucacgaag     1620

gagaucgagc agaagcugug gcacaucauc uacuccguga ccgacaagaa cgaguucgag     1680

aaggcccugc gcaccuucgc caagaaguac gaccucgaca ucgccagcuu cguggacaac     1740

uucaagaaga ucccgccguu caagaacgag uacggguccu ucagcgagaa ggccaucaag     1800

aagcugcugc cccucaugcg gcugggcaag uacuggaagu ggaacgacau cgacaacaac     1860

acccagaacc gcaucuccaa gaucaucacg ggcgaguacg acgaggagau caaggacauc     1920

guccgggaga aguccaucgc gcugaccaac gagaacgacu uccaggggcu ccagcugugg     1980

cuggcccagu accucgugua cggccgccac agcgaggccg acaucgccgg gaaguggcac     2040

agcgucgccg accuggagaa guaccugaac gaguucaagc agcacucccu ccgcaacccc     2100

aucguggagc agaucaucac cgagacccug cggguggucc gcgacaucug gcggaaguac     2160

ggccaggggg cggagaacuu cuucagcgag auccacgugg agcugggccg cgacaugaag     2220

aacacggccg acgagcggaa gaagaucgug aacgucguga ccgagaacga gaacaccaac     2280

cuccgcauca aggcccugcu gauggagcuc aagaacaacu ccgacggcaa gcuggaggug     2340

gagaacgucc ggcccuacag cccgacccag caggacaucc ugaagaucua cgaggaguac     2400

gccaucucca cggggcucga caacgagaag gacgagaagg ugaaggagga caucaagaag     2460

aucagccgcg uggcccagcc caccaccacc gagcugcagc gguacaagcu guggcucgag     2520

cagaaguacu gcucccccua cacgggcaag gucauccccc uggggaagcu guucaccgag     2580

gaguaccaga ucgagcacau cauccccaag agccgcuacu acgacgacag cuucuccaac     2640

aaggugaucu gcgaggcggc cgugaacaag cucaaggaca agugccuggg ccuggaguuc     2700

aucaagaacu accacgggca gaucgucgag accggcuucg gccagaaggu gaccaucuuc     2760

gacgaggagg ccuaccagaa cuucgugaag cagcacuacg ccuacaaccg gagcaagcgc     2820

accaagcucc ugcuggagga gauccccgag aagaugaucg agcggcagau gaacgacacg     2880

cgcuacaucu ccaaguucgu ccucccccug cugagcaacc ucgugcgggc ggaggagaac     2940

gacaacgggg ugaacuccaa gaacguccug cccgugaacg gcaagaucac caccaugcug     3000

aagcaggacu gggggcucaa cgacgugugg aacgaccuga uccugccgcg cuucguccgg     3060

augaacgagc ucgccaagac caucgccuuc acgagcugga acgagcagca ccagaaguac     3120

cugcccaccg ugccccugga gcucuccaag ggcuuccaga agaagcgcau cgaccaccgg     3180

caccacgcca uggacgcccu ggugaucgcg ugcgccaccc gcgaccacgu caaccugcuc     3240

aacaacaagc acgccaacac cgacacgauc cgguacgacc ugcagcgcaa gcugcgccuc     3300

uucgagcggg ugaccuacau cgacccgcag accaagaaca acgugaccaa ggacaucccc     3360

aaggaguuca agaagcccug ggacaacuuc acggucgacg cccgcaacga gcuggagaag     3420

aucaucguga gcuucaagca gaaccugcgg aucaucaaca aggccaccaa caucuacacc     3480

aaguacgaga acggcaagaa gaucaaggug gggcagaagg gccucaacug ggcgauccgc     3540

aagccccugc acaaggagac cgucuucgcc aagguguccc ugcggaagcg caagacggug     3600

cggcucagcg aggcccugaa ggacuggaag aagaucgucg acaagaagcu gaagcaggag     3660

aucaagcgcc ugaccugcca guacgggaag uucgacgugg acaccauccu gcgguacuuc     3720

aaggaccgca aguaccaguu cggcgaggug gacgucucca aggugaagau guacuacuuc     3780

gacgaggaga acgccgccgu gcggaagaac gucgacacca gcuucacgga gaaguucauc     3840

cagggguccg ugaccgacac cggcauccag aagauccugc ucaaccaccu ggaggcgaag     3900

ggcaacaagg uggagaucgc cuucagcccg gaggggaucg aggagaugaa caagaacauc     3960

auccagcuga acgagggcaa gcuccaccuc cccaucuuca agguccgcgu guacgagacc     4020

aucgggaaca aguucuccgu gggcgucaag gggaacaaga aggacaagua cguggaggcc     4080

gccaagggca cgaaccuguu cuucgcgauc uacguggacg agaacggcau ccggagcuac     4140

gagaccaucc cccucaacau cgucaucgag cgccugaagc aggggcuguc cguggucccc     4200

gagaagaacc ggaaggagca cagccuccug uucuaccuga gcccgaacga ccucguguac     4260

gugcccaucg aggacgagcg cgagaacauc cacgccguca accuggacca gcugaacaac     4320

gagcagcgga agcgcaucua caagauggug uccugcaccg gcagcgagug ccacuucgug     4380

cccuacuacg ucgccucccc caucgugaac aagguggagu acagcucccu caacaagauc     4440

gggcggagcc ugaccggcga gaugaucaag gacgucugca ucaagcugaa gguggaccgc     4500

cucgggaacg ugaagggcau cuccggccug uga                                  4533


<210>  2271
<211>  4536
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2271
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cgugaucaac       60

agcgucgccg acgagagcgu ggagaagcgc aacaucgagc uggcgggguc ccggaucauc      120

cccauggacg ccgccgugcu cggcaacuuc gacaacgggg ucaccaagag ccagacggcc      180

gagcgcaccu ccuaccggag cauccgccgg cugcgcgagc ggcagcugcu ccgccgggag      240

cgccugcaca aggugcugca gauccucggc uuccugccgg agcacuacgu gaacgacauc      300

gacuucgaga accaccccgg caagaucaag aagaacaccg agcccaagcu gcccuggcgg      360

aagaacucca uggggcaguu cgaguucauc uuccaggcca gcuucgaggc gaugcucacc      420

gacuucgcca cgcaccagcc ggucuucgug uccgagggca agaaggugca cuacgacugg      480

acccuguacu accugcgcaa gaaggcccuc accgaggaga ucagcaagga ggagcuggcc      540

uggaucaucc ugaacuucaa ccagaagcgg ggguacuacc agcuccgcgg cgaggaggag      600

gaggagcagu ccaacaagcu ggucgaguuc uacgcgcugc ggguggugaa ggucgaggcc      660

accgacgaca agaaggggaa ggacacgugg uacaacgugc accucgagaa cggcuggauc      720

uaccgccgga ccagcaacau cccccuggac ugggagggca aggugaagga guucaucguc      780

accaccgacc ugaacgagga cgggacgccc aagcucgaca aguacggcga ggugaagcgc      840

uccuuccggg cccccaagga ggacgacugg acccugauca agaagaagac cgagagcgac      900

aucgccaacu cccacaagac ccuggggugc uacaucuacg acgcccuccu gcagaacccg      960

aaccagaaga ucaagggccg ccuggugcgg acggucgagc gcaaguucua caaggaggag     1020

cuccgccuga uccuggagaa gcagcucagc uuccaccccg agcugcagga ccgggaccug     1080

uaccgcgcgu gcaucgacgc ccucuacgug uccaacgagg cccacaagaa caacaucagc     1140

ggccgggacu ucguguaccu guucaucgac gacaucaucu ucuaccagcg cccccugaag     1200

accaagaagu cccucaucga caacugcccc uacgagaaga accaguacau ggaccgggag     1260

accggggagg ucaagcaggc cccgaucaag ugcaucgcca agagccaccc ccuguuccag     1320

gaguuccgcc uguggcaguu caucagcaac auccggaucu accagcgcga gcgggugaug     1380

gacgacggca agcucgagac cgacguggac gucacgcccg acuuccugaa gaccgaggac     1440

gacuacacca cccuguucga guggcucaac gaccgcaagg agguggacca gaaggcguuc     1500

cugaagaacc cccaguucgg gcugaagaag acguucaccg aguaccggug gaacuacguc     1560

gaggacaagu ccuacccgug caacgagacc cgcaacgcca uccucaacgg ccugcagaag     1620

gccgggguga gcgccgacuu ccugucccgg gagguggagg aggcccugug gcacauccuc     1680

uacagcgucg aggacaagau cgagaucgag uccgcgcugc gcaaguucgc cagcaagcac     1740

gaccuggacg accgguucgc cgacguguuc aagaagcucc cgcccuuccg caaggaguac     1800

ggcgccuacu ccgccaaggc gaucaagaag cugcugcccc ucaugcggau gggcaaguac     1860

ugggagcuga gcaacaucga cgacaagacc aaggagcgca ucgacaagau caucacgggg     1920

gagugggacg agaacaucca caaccgggug cgcgagaagg ccaucaagcu guccgacauc     1980

accgacuuca agggccuccc ccuguggcug agcugcuacg ucguguacaa ccgccacucc     2040

gaggcccggg ccaucgcgaa gugggagagc cccgaggaca ucgacuccua ccuccgccug     2100

uuccggcagc acagccugcg caaccccgug gucgagcagg ucaucaccga gacccuccgg     2160

acggugcgcg acaucuggaa gcggaucggg cagaucgacg agauccacgu ggagcugggc     2220

cgcgagauca agaaccccgc cgacaagcgg gccaagauga ccgcccagau cguggagaac     2280

gagaacacca accugcgcau caaggcccuc cuggcggagu ucaucaacuc cgaguacgag     2340

aucgagaacg ugcggccgua cagccccggg cagcaggaga uccugcgcau cuacgaggac     2400

accguccuca acuccgugga ggagaucccc gaggacauca gcgccgugcu gaagaaguuc     2460

cgggaguccg accugaagaa gcgccccacc aagagcgagu uccuccggua caagcugugg     2520

cuggagcaga aguaccgcag cccguacacg ggcgacauca ucccccucgg caagcuguuc     2580

accuccgccu acgagaucga gcacaucauc ccccagagcc gguacuucga cgacuccuuc     2640

agcaacaagg ucaucugcga ggccgaggug aacaagcuga aguccaacga gcucggguac     2700

gaguucauca agaaccacca cggccaguac guggagaucg gguucggcaa ggucgugcag     2760

aucuucagca ucgaggccua cgagaaguuc gugaaggcga acuacuccgg gaucggcagc     2820

aagaugaagc gccugcugau ggacgacauc ccggagaccu ucaucgagcg gcagcucaac     2880

gacucccgcu acaucagcaa ggucgugaag ucccugcuga gcaacaucgu gcgggagaag     2940

gacuccaacg gcgaguacga gcccgaggcc aucagcaaga acgucaucgu gugcaccggg     3000

uccgugacgg accgccucaa gcgcgacugg ggcgucaacg acguguggaa cagcaucauc     3060

uacccccggu ucgagcgccu gaacgagcug accggguccc agcaguucgg cugcugggag     3120

aacaaggagg gcaagcgggu guuccagacc gccguccccc ucgagaugca gaagggguuc     3180

agcaagaagc gcaucgacca ccggcaccac gccauggacg ccaucgugau cgcgugcgcc     3240

auccgcaacc acaucaacua ccugagcaac gaguccgcca gcaagaaggc caagaucucc     3300

cggaucgacc ugcagcgccu ccugugcacc aagugccgga cggacgacaa cggcaacuac     3360

aaguggaucg ugaagaagcc guggcccgcg uucacccagg acauccgcaa ggcccuggag     3420

aacaucaccg ucagcuucaa gcagaaccuc cgggugauca acaagaccac gaacuacuac     3480

gugcacuucg acgaguccgg gaagaagguc cuggagaagc agaucaacgg cgacagcugg     3540

gccauccgca agcccaugca caaggacacc guguucggga aggugaaccu gcagaagguc     3600

aaggaggugc ggcucucccu ggcccuggac acccccaaga ugaucgugga caagaagcuc     3660

aaggagaagg uccugcagcu guacagcuac aaguacgaca agaagcgcau cgacaaguac     3720

uucaaggaga acgccucccu cuggaaggac cugaaccuga gcaagguggc gguguacuac     3780

uucaccaacg acuccacgga gccgcucguc gccgugcgga agccccugga caccagcuuc     3840

aacgccaaga agaucaagga guccgugacc gacaccggca uccagaagau ccugcucaac     3900

caccugagca acaaccagga caaccccaac cuggccuucu cccccgacgg caucgacgag     3960

augaaccgca accucacgac ccugaacgac gggaaguacc accagccgau cuacaagguc     4020

cgggugugcg agccccaggg caacaaguuc agcgucggga ccuccggcaa caagaccagc     4080

aaguacgugg aggccgcgaa ggggacgaac cuguucuucg ccguguacca gaccgaggac     4140

ggcaagcgca acuacgagac caucccccuc aacgucguga ucgagcggga gaagcuggag     4200

ugggcccccg ugccggagaa caacaacaac ggcgacaagc uccuguucug gcuguccccc     4260

aacgaccugg ucuacgugcc caccgccgac gagcucaaca acgggaugcg ccuggacaac     4320

auggacaagg gccgcaucua caagaugauc agcugcagcg ggccccagug cuucuucauc     4380

ccgcagaacg uguccaaccc caucaucccc gucaucgagc ugggcgugaa caacaaggcc     4440

gagaaguccu ggaacaacga gaugaucaag guggucugcg ugcccauccg gguggaccgc     4500

cucgggugca ucacgcagau caacaaccag aaguga                               4536


<210>  2272
<211>  4539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2272
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucgag       60

aacuccuuca ccgagaagga ggggaagauc aacgacaugg gcgugcgcau cauccccaug      120

agcgccgaca uccucgggaa guucgacgcg ggccaguccc acagccagac ggccgagcgg      180

accggcuacc gcgggacccg gcgccuguac cagcgggaca accugcgccg ggagcgccuc      240

caccgggucc ugcacauccu gggcuuccuc ccgaagcacu acgacgagca caucgacuuc      300

gagaagcgcc uggggcaguu caaggagggc aaggagauca agcugaacua ccggugcaac      360

guggagaaca agcacgaguu caucuucauc uccagcuuca acgagaugcu caccgaguuc      420

aagaaggccc agcccgagcu guucuaccug aaguccaacg gggaggagac gaagaucccc      480

uacgacugga cccucuacua ccugcgcaag aaggcccuga gccagccccu caccaagcag      540

gagcuggccu ggaucauccu gaacuucaac cagaagcggg gcuacuacca gcuccgcggc      600

gaggagaucg acgacgacaa gaacaagcag uucgugaagc ugaaggucaa ggaggugauc      660

aacuccgggg aggcggugaa gggcaagccg cuguucgacg ucaucuucga gaacgggugg      720

aaguacgaca agcaggucgu gaagaccgag gacuggaucg accggacgaa ggaguucauc      780

gugaccacca aggugcucaa gaacggcgac cugaagcgca gcuacaagac cguggacucc      840

gagaaggacu gggccgccau caaggccaag acggagcagg acaucgagcg gagcaacaag      900

accgucggcg aguucaucua cgagacccug cucgagaacc ccacccagaa gauccgcggg      960

aagcugguga agacgaucga gcggaaguuc uacaaggagg agcugaagaa cauccucauc     1020

aagcagaucg agcugcagcc cacccuguuc uccgaggagc ucuacaaggc gugcaucgag     1080

gagcuguacc cccgcaacga ggcccaccag aacgccauca agaaccggga cuucaucuac     1140

cugcucaugg acgacaucau cuucuaccag cgcccgcuca agagccagaa guccaacauc     1200

agcggcugcc agcuggagca gcgcguguac uacaaggcca acaaggacuc cggggagaac     1260

gaggagguca agcaggccgu gaaggcgauc cccaagagcc acccccuguu ccaggaguuc     1320

cggaacuggc aguggcugca gaaccugaag aucuacaaca agguggacac cgagaagggc     1380

gagcucaccg acgucacgaa gcagcugcug cccuccgagg acagcugggu gaaccucuuc     1440

aacuuccugc agugcaagaa ggagcuggag cagaagcagu ucaucaagua cuucaucgac     1500

aagaagcuca ucaacaagac cgagaaggac cacuaccgcu ggaacuacgu ggaggacaag     1560

aaguacccgu ucaccgagac ccgggcccag uucaucagcc gccugacgaa ggucgagggg     1620

aucgaggaga ucaacaccuu ccuggacaag aagacccaga ucggcaccaa ggacaacucc     1680

cccuucguga gccggaucga gcagcugugg cacaucaucu acuccgugag cgacaucaag     1740

gaguacgagu ccgcccucga gaaguucgcc aucaagcacc accugaacga ggagagcuuc     1800

gucgccaacu ucaucaaguu cccgcccuuc aaguccgacu acggcagcua cuccaagaag     1860

gcgcugaaca agcuccugcc ccugaugcgc cgggggaacu acuggacgga gaacgacauc     1920

agcgagaagg ugaagcuccg cgucuccgag aucauggagc gggugaacgc ccugaaccug     1980

aaggaggacu acagcuccaa ggagcucgcc gagacccugg ugaacgucag cgacgacgac     2040

gugaagaagc agcugaucaa guccuucgug agcuucaagg accgcaaccc ccucaugggc     2100

cugaacaccu uccaggccac cuaccugguc uacgggcggc acuccgaggu gggcgacauc     2160

cagaacugga agacgcccga cgacaucgac accuaccuca agaacuucaa gcagcacagc     2220

cugcgcaacc ccaucgugga gcaggucgug accgagaccc ugcggguggu ccgcgacauc     2280

uggaagcacc acgggaaguc ccagacgaac uucuucaacg agauccacgu ggagcucggc     2340

cgggagauga agaacccggc cgacaagcgc aagcggauca gcgagcgcaa caccgagaac     2400

gagaacacca acaaccggau ccgcgagauc cugaaggacc ugaugaacga cgcgagcauc     2460

cagggcgacg ugcgcgacua cucccccagc cagcagggga uccucaagau cuacgaggag     2520

ggcgucuacc agaaccccaa gguggacuac uccaagguga gcgaggacga gaucaccaag     2580

auccggcgcu ccaacaaccc cacccagaag gagauccagc gguaccgccu guggcuggag     2640

cagggguaca ucagcccgua cacgugcaag cccauccccc ucuccaagcu guucacccac     2700

cacuaccaga ucgagcacau caucccccag agccgguacu ucgacaacuc cagcuccaac     2760

aagaucaucu gcgagagcga ggucaacgag gacaaggaca acaagaccgc cuucgaguac     2820

cugaaggaca agggcgagaa cgugaucaac gggcacaagc uccugucccu ggccgccuac     2880

gaggcccacg ugaacaagua cuucaagaac aaccgccaga agcucaagaa ccugcugagc     2940

gaggacaucc cggagggcuu caucaaccgg cagaugaacg acucccgcua caucagcaag     3000

cucgucaagg gccugcuguc caacaucgug cgggaggagg gggagcagga ggugaccagc     3060

aagaaccuca uccccgucac gggcagcgug accuccaagc ugaagaacga cugggggcug     3120

aacgacaagu ggaacgagcu cauccugccc cgcuucaagc ggcugaacca gcucacccag     3180

accaacgacu ucacgaccgc gaacaccaac ggcaacacca ucccgacggu gcccgacgag     3240

cugcugaagg gcuucagcaa gaagcgcauc gaccaccggc accacgcccu cgacgcccug     3300

gucguggccu gcugcacccg caaccacucc aacuaccuga gcgcgcucaa caccgagaac     3360

aagaacuacg ggcugcggga caagcugcuc aucaagaacg acaacgacca cuacaccaag     3420

uccuuccaga ugcccuggca cggcuucacg accgaggcca agaaccagcu ggaggagacc     3480

gugaucagcu ucaagcagaa ccugcgcguc aucaacaagg ccaacaacaa guucuggucc     3540

uacaaggacg agaacgggaa gcucaaccug gacaagaacg gcaagcccau caagaagcug     3600

cggaagcaga ccaaggggga caacugggcc gugcgccagc cgcuccacga ggagacgauc     3660

agcggcaucg ugaaccugcc cugggucaag gccgagaagg agaaguucac cuacgcgacc     3720

cggaaguccc uggacaagag cuucgaccuc aagaagaucg agaagaucac cgacacgggc     3780

auccagaaga uccugcgcaa cuaccugaug caggagaagu ucaaggagcu cgacaagaag     3840

gacaaggagg uguacaacuc cgagcuggcc uucagccagg aggggcugga ggaccucaac     3900

uccaacaucg agcaguucaa cgaccagaag ccccacaagc ccaucuacaa ggugcggcug     3960

uucgagaagg gcagcgggcg cuucgcccug ggcuucaagg ggaucaacgc caagaaguac     4020

gcccagggcu ccccgaaccu cuacuucaac aucuaccgca acgaggacgg ccaguacuac     4080

gagaccgucc cccugaacaa ggugaucauc caccagaaga acgcggccaa caugcccaag     4140

aagaaccgga cccccgugcc gaucagcaac accgccauca accgcuccaa ggagguccug     4200

guggacuaca agaugacgcu cagcccccug gaccuggugu acauccccac cgaggaggag     4260

cucaacaaca ucaacucccu ggacuucaac aaccugagca agagccagcg gaagcgccuc     4320

uacaacguca acgacuucuc cggguacacc aucuacuuca gccccaacac ccuggccaag     4380

uccaucugcc cgaaggaggu ggacaugaag uucgacgaga agaagcagaa gcccacgggc     4440

agcuucgacu ccaagaccgc cagccuggac gggaugucca ucaaggagcg gugcaucaag     4500

cucaucaucg accgccuggg caacaucagc aaggcguga                            4539


<210>  2273
<211>  4539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2273
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cguggucaac       60

gccgacgaga ucacccgcga cgacgggucc cgguaccuga agcccaacgg caucagcgcg      120

gccggguccc gcaucauccc gaugagcgcc gacauccucg gcgacuucga gaagggcaac      180

uccaucagcc agacggccga gcggacccgc augcggaugg cccgccggcu gcacgagcgc      240

gcgcugcucc ggcgcgagcg gcugcugcgc gugcucaacc ugcuggacuu ccuccccaag      300

cacuucgccg acaagaucga ccgguacggg aaguucaccg acgacuccga gcccaagcug      360

gccuggcgca agaacaccga gggcaaguac gaguucaucu uccaggacgc cuucaacgag      420

augcuggcgg aguucaagga caagcagccc gagaucgugg aggaggggaa gaagaucccg      480

uacgacugga cgaucuacua ccuccggaag aaggcccugg agaaggcccu gagcaaggag      540

gagcucuccu ggauccugcu gcaguucaac cagaagcgcg gcuacuacca gcuccgcggg      600

gaggaggagg agauccccca ggacaagaag aucgaguacc uggcccagaa ggucgugaag      660

guggaggcca ccgaccagaa gaagggcgac gacgucuggu acaacgugua ccuggagaac      720

ggcaugaucu accggcggac cagcaaggcg ccccucgacu gggaggggaa ggugaaggag      780

uucaucguca ccacggaggu ggagaacgac ggcgagaccc ccaagaagga caaggagggg      840

aacaucaagc gcuccuuccg ggccccgaag gaggacgacu ggacccugcu gaagaagaag      900

accgaggccg acaucgagaa cagccacaag acggugggcu gcuacaucua cgacucccuc      960

cugaacaacc ccaagcagaa gaucaucggc aagcuggucc gcaccgugga gcgcaaguuc     1020

uacaaggagg agcucaccca gauccugaag aagcaggucg agcugauccc cgagcuccgg     1080

gacgacaacc uguacaagca gugcaucgag gagcuguacc ccaucaacga ggcccaccgc     1140

aacaccaucg ccaagacgga cuucgcgaac cucuucauca acgacauccu guucuaccag     1200

cggccgcuca agagcaagaa gucccagauc gacaacugcc ccuacgagga gcgcaucuuc     1260

aucgacagca agaccgggga gaagaagaag gugcccguga agugcaucgc caaguccaac     1320

ccccuguucc aggaguuccg gcuguggcag uucauccaga accugcgcau cuaccagcgg     1380

gagaaggaga ucgacggcaa gcuccugacc gacaucgaca ucaccagcga gugccugaag     1440

uccgaggagg acuacgugcg ccucuucgac uggcugaacg accgggagac gaucgagcag     1500

gaggaccugc ucaagcaccu guucaacacc aagaagucca agaacaagga gaacccguac     1560

cgcuggaacu acgucgagga caagguguac cccugcaacg agacccgggc caccauccug     1620

aagggguuca gcaagugcgg caucaacgcc agcgugcuga gccccgagcu cgagauggcc     1680

cuguggcaca uccuguacuc cgucgaggac aagaaggaga ucgagacggc gcucacccac     1740

uucgcccaga agcaggggug gaacgacgac uucgccaagg uguucagcaa ggccaagccc     1800

uucaagaagg acuacggcuc cuacagcgag aaggccauca agaagcugcu gucccucaug     1860

cgcaugggca aguacuggaa ccaggacaac aucgacaaga acacccugga ccggaucgac     1920

aagaucauca acggggagua cgacgagaag aucagcaacc gcgugcggga caacgcgauc     1980

aaccugaagg acaucuccga cuuccgcggc cucccggucu ggcuggccug cuacaucgug     2040

uacgaccggc acagcgaggc caaggacugc accaagugga acucccccga ggagaucgac     2100

agcuaccuga agaaguucaa gcagcacucc cuccgcaacc ccaucgucga gcaggucgug     2160

acggagaccc ugcggaccgu gcgcgacauc uggauccagg aggagcagau cgacgagauc     2220

caccuggagc ucgggcgcga ccugaagaac cccgccgaca agcggaagaa gaugagcgag     2280

aacauccuga agaacgagaa caccaaccuc cgcaucaagg cgaugcugau ggaguucaug     2340

aacccgucca ugggcaucga gaacgugcgg cccuacagcc ccucccagca ggacauccug     2400

cgcaucuacg aggagaacgc ccucgacaac cugacgaagg acgacaagga cuucgacuuc     2460

aucagcaaga ucuccaagca ggcccagccc agcaagaccg acaucauccg guacaagugc     2520

uggcuggagc agaaguaccg cagcccguac accgggaaga ccaucucccu cagcaagcug     2580

uucacgcccg ccuacgagau cgagcacgug aucccccagu cccuguacuu cgacgacagc     2640

uucuccaaca aggucaucug cgaggccgag gugaacaagc ucaaggaccg gcagcugggc     2700

uacaaguuca ucgcggagca ccacggcgag aaggugcagc ugagccaggg ggaggucgug     2760

gagauccucu ccguggacgc cuacgagaag uucgucaagg agaacuacgc caacaaccgc     2820

aucaagauga agaagcugcu gauggagagc aucccggacg aguucaucga gcggcagcuc     2880

aacgacuccc gcuacaucag caagguggug aagggccugc uguccaacau cguccgggag     2940

aagaucgacg acgagaacua cgagcccgag gccgugagca agaaccucau cuccugcaac     3000

ggggccauca ccgaccgccu gaagaaggac uggggcauga acgacgugug gaacagcauc     3060

auccugcccc gguucauccg caugaaccag aucaccggga aggacugcuu caccaccacg     3120

aacgcggagg gccaccucau cccccagaug ccgcuggagc ugcagaaggg cuucaacaag     3180

aagcggaucg accaccgcca ccacgccaug gacgccaucg ucaucgccug caccacccgg     3240

gaccacguga accuccugaa caacgaggcc gcgcacucca aguucaacgc cacccgcuac     3300

cagcugcagc ggaagcuccg ccgguucgag aaggccguga ucgacgggaa ggagcgcgag     3360

gucgccaagg aguuccugaa gcccugggac agcuucucca gcgacagcaa gaacauccug     3420

gagaacauca ucguguccuu caagcagaac cagcgcguga ucaacaagac gaccaacgcg     3480

uuccuccacu ucgacgagaa cggcaagaag accuucauca agcaggggaa gggcgacagc     3540

ugggccaucc ggaaguccau gcacaaggac accgucuucg gcgagaucaa ccugcgcaag     3600

gugcggagcg ugucccugaa cgaggccauc aagguccccg agcgcgugcu caacaagaag     3660

aucaaggaga agauccugga gcugaagaac aacaagaucg acgccaagaa caucaagaag     3720

uacaucgagg agaaccacac cgggggcuac gggaucgaca gcuccaagau cgacguguuc     3780

uacuucacga aggagaccaa ggagcgguuc uucgccaccc gcaagacgcu cgacaccagc     3840

uucaaccagg cgaagaucaa ggacuccauc gccgacagcg gcauccagaa gauccugcug     3900

gcccaccuca aguccaagaa cggggacgcc gagcaggccu ucagccccga cggcaucgac     3960

gagaugaaca agaacaucgu cgagcugaac aacggcaagu uccaccagcc gauccugaag     4020

gugcgggugu acgagaaggc ggacaaguuc gccgucgggc agaagggcaa caagaaggug     4080

aaguucgugg aggccgccaa ggggaccaac cucuucuucg ccaucuucga gaaggacggc     4140

aagcgcuccu accugaccau cccccugaac gucaugaucg acugccagaa gcaguacggg     4200

aaccagugga agaacaacau cgaggcguac cucaaggaga aggagcuggu ggagaaggac     4260

gugaagcugc aguucauccu cagccccaac gaccuggucu accugcccac ggagggcucc     4320

aucgacaaga agcggaucua caagguggug agcuucacca acaaccgccu cuacgccauc     4380

ccguacgcca ucgccaaguc caucgucgac aagaacgagu ucacccagcu gaacaagauc     4440

gaguucaccg acaacaagga gagcaucaag gacgcgugcg ugcccgugau caucgaccgg     4500

cugggcaacg ucaucgaguu caacgggaag cgccucuga                            4539


<210>  2274
<211>  4539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2274
augaagaaca uccugggccu cgaccugggg accaacagca ucggcugggc cgugaucaac       60

agcgaggagg acgggcccau cgucaagaag cgcaucgagc uggccggcag ccggaucauc      120

ccgauggacg cggccgugcu cgggaacuuc gacuccggcg ugaccaagag ccagacggcc      180

gagcgcaccu ccuuccggag cauccgccgg cugcgcgagc ggcagcugcu ccgccgggag      240

cgccugcaca agguccugca gauccucggc uuccugcccg agcacuacgu gaacgacauc      300

gacuucgaga accaccccgg gaagaucaag cggaacaccg agcccaagcu gccguggcgc      360

aagaacucca ugggccaguu cgaguucauc uuccaggaca gcuucgaggc caugcucacc      420

gacuucgcca cgcaccagcc cguguucguc uccgagggga agaaggugcc cuacgacugg      480

acccuguacu accugcggaa gaaggcgcuc accgagggca ucagcaagga ggagcuggcc      540

uggcugaucc ucaacuucaa ccagaagcgc ggguacuacc agcugcgggg cgaggaggag      600

gaggagcagu ccaacaagcu gguggaguuc uacgcccucc gcgucgugaa gguggaggcc      660

accgacgaca agaagggcaa ggacacgugg uacaacgucc accuggagaa cggguggauc      720

uaccggcgca ccagcaacau cccccuggac ugggagggca aggugaagga guucaucgug      780

accaccgacc ucaacgagga cgggacgccg aagcuggaca aguacggcga ggucaagcgg      840

uccuuccgcg cgcccaagga ggacgacugg acccugauca agaagaagac cgagagcgac      900

aucgccaacu cccacaagac cgugggcugc uacaucuacg acgcccuccu gcagaacccc      960

caccagaaga ucaaggggcg gcuggugcgc acggucgagc gcaaguucua caaggaggag     1020

cuccggcuga uccuggagaa gcagcucagc uuccaccccg agcugcagga ccgcgaccug     1080

uaccgggccu gcaucgacgc ccucuacgug uccaacgagg cgcacaagaa caacaucagc     1140

ggccgcgacu ucguguaccu guucaucgac gacaucaucu ucuaccagcg gccgcugaag     1200

accaagaagu cccucaucga caacugcccc uacgaggaga accaguacau ggaccgcgag     1260

accggggagg ucaagcaggc ccccaucaag ugcaucgcca agagccaccc ccuguuccag     1320

gaguuccggc uguggcaguu caucagcaac auccgcaucu accagcggga gcgcgugcuc     1380

gacgacggca agcuggagac cgacguggac gucacgccgg acuuccugaa gaccgaggac     1440

gacuacacca cccucuucga gugguucaac gaccggaagg agguggacca gaaggccuuc     1500

cugaagaacc cccaguucgg gcugaagaag acguucaccg aguaccgcug gaacuacguc     1560

gaggacaagu ccuaccccug caacgagacc cggaacgcca uccucaacgg ccugcagaag     1620

gcgggcguga gcgccgacuu ccugucccgc gagguggagg aggcccugug gcacauccuc     1680

uacagcgucg aggacaagau cgagaucgag uccgcccugc ggaaguucgc cagcaagcac     1740

gaccuggacg accgcuucgc ggacguguuc aagaagcugc ccccguucaa gaaggaguac     1800

ggggccuacu ccgccaaggc caucaagaag cugcugcccc ucaugcggau cggcaaguac     1860

ugggagcuga gcaacaucga cgacaagacc aaggagcgca ucgacaagau caucacgggg     1920

gagugggacg agaacaucca cgaccgggug cgcgagaagg ccaucaagcu guccgacauc     1980

accgacuuca agggccuccc ccuguggcug agcugcuacg ucguguacaa ccgccacucc     2040

gaggcgcggg ccaucgccaa gugggagagc cccgaggaca ucgacuccua ccuccgccug     2100

uuccggcagc acagccugcg caacccgauc guggagcagg ucaucaccga gacccuccgg     2160

acggugcgcg acaucuggaa gcgggugggg cagaucgacg agauccacgu cgagcugggc     2220

cgcgagauca agaaccccgc cgacaagcgg gcgaagauga ccgcccagau cguggagaac     2280

gagaacacca accugcgcau caaggcccuc cuggccgagu ucaucaacuc cgaguacgag     2340

aucgagaacg ugcggcccua cagcuccaac cagcaggaga agcugaagau cuacgaggac     2400

accguccuca acggcgugaa ggagaucccc gaggacauca acacgauccu gaagaaguuc     2460

cgcgagagcg accugaagaa gcggccgacc aaguccgacu uccuccacua caagcugugg     2520

cuggagcaga aguacugcag ccccuacacc gggcgcauca ucccccucgg caagcuguuc     2580

accagcgccu acgagaucga gcacaucauc ccgcaggcgc acuacuucga cgacuccuuc     2640

agcaacaagg ugaucugcga ggccgagguc aacgggcuga aggacaagaa gcucggcuac     2700

gaguucauca agcacaaccc cgggaagaug guggagaucg gcuucggcaa gccgcccgug     2760

cggaucuucu ccgucgagga guacgagcgc uucgugaagg agaacuacag cgggaucaag     2820

aacaagauga acaagcugcu gauggacgac auccccgaga ccuucaucga gcggcagcuc     2880

aacgacuccc gcuacaucag caaggugguc aagucccugc ugagcaacau cgugcgggag     2940

aaggacucca acggcgagua cgagcccgag gccaucagca agaacgugau cgucugcacg     3000

ggguccguga ccgaccgccu caagcgggac uggggcguga acgacgucug gaacagcauc     3060

aucuaccccc gcuucgagcg ccugaacgag cugaccggcu cccagcaguu cgggugcugg     3120

gagaacaagg agggcaagcg gguguuccag accgccgugc cccucgagau gcagaagggg     3180

uucagcaaga agcgcaucga ccaccggcac cacgccaugg acgcgaucgu caucgccugc     3240

gccacgcgca accacaucaa cuaccugucc aacgagagcg ccuccaagaa ggccaagauc     3300

agccggaccg accugcagcg ccuccugugc accaagugcc ggaccgacga caacggcaac     3360

uacaagugga ucgugaagaa gccguggccc gcguucacgc aggacauccg caaggcccug     3420

gagaacauca ccgugagcuu caagcagaac cuccggguca ucaacaagac caccaacuac     3480

uacgugcacu ucgacgaguc cgggaagaag gugcuggaga agcagaucaa cggcgacagc     3540

ugggccaucc gcaagcccau gcacaaggac acggucuucg gcaaggugaa ccugcagaag     3600

gugaaggagg uccggcucuc ccuggcccug gacaccccca agaugaucgu ggacaagaag     3660

cucaaggaga aggugcugca gcuguacagc uacaaguacg acaagaagcg caucgacaag     3720

uacuucaagg agaacgcguc ccucuggaag gaccugaacc ugagcaaggu cgccguguac     3780

uacuucacca acgacuccac cgagccgcuc guggccgucc ggaagccccu ggacacgagc     3840

uucaacgcca agaagaucaa ggaguccgug accgacaccg ggauccagaa gauccugcuc     3900

aaccaccuga gcaacaacca ggacgacccc aaccuggccu ucucccccga cggcaucgac     3960

aagaugaaca agaaccucac cgcgcugaac gacgggaagu accaccagcc gaucuacaag     4020

gugcgcgucu gcgagcccca gggcaacaag uucagcgugg ggacguccgg caacaagacc     4080

agcaaguacg ucgaggccgc caagggcacc aaccuguucu ucgccgugua ccagaccgag     4140

gacgggaagc gguccuacga gacgaucccc cucaacgugg ucaucgagcg cgagaagcug     4200

ggcuggaccc ccgugccgga gaacaacaac aacggggaca agcuccucuu cuggcugagc     4260

cccaacgacc ugguguacgu ccccaccgcc gacgagcuca acaacggcau gcggcuggac     4320

aacauggaca aggggcgcau cuacaagaug aucuccugca gcggccccca gugcuucuuc     4380

aucccgcaga acguguccaa cgcgaucauc cccgugaucg agcugggcgc caacaacaag     4440

gccgagaagu ccuggaacaa cgagaugauc aaggucgugu gcgugcccau ccgggucgac     4500

cgccucgggu gcaucaccca gaucaacaac cagaaguga                            4539


<210>  2275
<211>  4551
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2275
augaagaaca uccugggccu cgaccugggg accaacagca ucggcugguc cgugauccag       60

gagaagcaca acgagaacaa cgagugccag aagcugaucc ucggggccgg cagccgcauc      120

auccccaugg acgccgcggu ccugggggac uucgccaagg gcaacaccau cucccagacg      180

aaggaccgga cccaguaccg cggcgugcgg cgccugcggg agcgcugccu ccugcggcgc      240

gagcggcugu uccgcgugcu cgacaucaug ggguuccugc cgcagcacuu cagcaacgcc      300

cugacccggu acggcaaguu caaggacgcc gacgaggagc ccaagaucgc cuggcgcaag      360

gacgacgagg ggaagaugca cuuccucuuc gcggacuccu ucaacgagau gaucgaggag      420

uuccggagcg uccagcccga gcugcugaag cgcggcauga aggugcccua cgacuggacc      480

cucuacuacc ugcggaagaa ggccaugcgc gaggccguga cgccguucga gcuggccugg      540

cucauccugu ccuucaacca gaagcggggg uacuaccagc ugcgcggcga ggaggagggc      600

gaggcggaca acagcaaguc cgaggaguac cucgcccagc gggucgugga cgugaucgac      660

accggggaga agaagggcaa gagcaccugg uucgacaucg uccuggagaa cgggauggug      720

uaccaccgcc ccgccgagau caagcccgac uggaucggca agaccaagga guucaucgug      780

acgacccagu ucgacaagga cggcaacccc aagaaggaca aggaggggaa cgucaagaug      840

uccuuccgga ugccgaacga gaacgacugg aaccugcaga agaagaaggc cgagaaggac      900

aucgacgaga gcggcaugac cgugggggag uacaucuaca acgcccuccu gcacaacccc      960

aagcagaaga ucaagggcaa gcuggugcgc gucguggagc gcaaguacua caagaacgag     1020

cuccagaaga uccuggaguc ccagaagcgg uucauccccc agcugagcga cuccgcgcuc     1080

uacgagaagu gcaucaagga gcuguaccag agcaacgagg ccuaccgcaa cuccaucagc     1140

acccggaacg acuucacgua ccuguucgug gacaacauca ucuucuacca gcgcccccuc     1200

aaguccaaga agucccugau cgacaacugc ccguacgaga gccuggccua caccaaggac     1260

ggggaggagc acaaggcccc cgucaagugc aucgccaaga gccacccccu cuaccaggag     1320

uuccggcugu ggcaguucau cagcaaccug cgcaucuacc agcggcagaa gcaggucggc     1380

gacaagguga agcucgacgu ggacgugacc gacgaguucc ugcccaccga cgaccgccgg     1440

gagucccugu ucgacuaccu caacgacaag acggagauca agcaggacgu gcugcugaag     1500

gaguucuuca gcaugaagaa gccgaagggc aaggacuccg ucuaccccua ccgcuggaac     1560

uacguggagg acaagagcua ccccugcaac gucacccggg ggaagauccu cgcgaagcug     1620

aaccgcgccg gcaucuccgc cgacuuccug agcuccaacg ggaacgagga gagccucugg     1680

cacauccugu acuccgugga ggacaagggc gagcugcgga aggcccuggc ccgcuucgcg     1740

gccaagaacg ggcucgacga cgccuucacc gaggcccugg ccgacaucga gcccuacaag     1800

aaggaguacg gcgcguacag cgccaaggcc cugaagaagc uccugccgcu gaugcggcuc     1860

ggcaaguacu ggaacuacga caacaucgcc cccgcgacca aggcccgcau cuccaagauc     1920

aucgacgggg aguacgacga gaacauccgg aaccgcgugc gggagaaggc caugagccug     1980

aacuccgcca gccaguucca gggccugccc cucuggcugg ccugcuacau cgucuacgac     2040

cgccacuccg agcgcgcgga cagcgggcgg ugguccagcc ccgaggacau cgacaaguac     2100

cugagcgacu ucaagcagca cucccuccgc aacccgaucg uggagcaggu cgugacggag     2160

acccugcgga ccgugcgcga caucuggaag cgguacggcg agaucagcga gauccacgug     2220

gagcuggggc gcgagaugaa gaaccccgcc gacaagcggu ccaagaugaa ccgccggaug     2280

cucgagaacg agaacgccaa caugcgcgcc aagcugcugc ucaccgaguu caugaacccc     2340

gaguucggca ucgagaacgu ccggagcuac ucccccagcc agcaggagcu gcugcgcauc     2400

uacgaggacg gcgccaucuc caacgcgggg agcgagaucg acgacgagau cuccgacauc     2460

augagccggc ucucccaggu ggacgugaag aagcagccga cggccagcga ggucaagcgc     2520

uacauccugu ggcuggagca gaacuaccgg ucccccuaca ccggccagau gaucccccuc     2580

gccaagcugu ucaccccggc cuacgagauc gagcacguga ucccccagag ccgcuacuuc     2640

gacgacuccu ucagcaacaa ggugaucugc gaggccgagg ucaacaagcu gaaggaccgg     2700

augcucgggu acgaguucau caagcagcac aagggcgaga aggugucccu gagcaugggc     2760

gggaccgugg agauccugag ccccgaggag uacguccgcc acguggaguc ccaguacaag     2820

aacaacaagg cgaagaugcg gaagcuccug auggacgaca uccccgacga guucaucaac     2880

cgccagcuga acgacagccg guacaucucc aagcucauca ucagccugcu guccaacauc     2940

gugcgcgagg agggcgagga ggaggccacg agcaagaacg ucaucuccug caacgggagc     3000

aucaccgacc gccucaagaa ggacuggggc aucaacgacg uguggaacca caucauccug     3060

ccgcgguucc gccggcugaa cgagcucacc gggaccgaca aguucacgac cuccaacaag     3120

gagggccacg aggugcccga gaugccccug gagcugcaga agggcuucaa caagaagcgc     3180

aucgaccacc ggcaccacgc cauggacgcc aucgucaucg cgugcaucac ccgcgaccac     3240

gugaaccucc ugaacaacga ggccgccaug agcaagaaca acgccaaccg gauccagcug     3300

uccaagaagc uccgccggua cgagagcgug gagaucgaga aggacgggau ccgcaagcag     3360

aucuccaugg ccaaggaguu caagaagccc ugggagggcu ucaccgcgga cgucgagcgg     3420

acgcugcgcg acaucguggu gagcuucaag cagaaccugc gggucaucaa caagaccacc     3480

aacuacuacc agcacuucaa ggacgggaag aagaucuacu ccccgcagag caagggcgac     3540

uccugggcca uccgcaagag caugcacaag gagaccgugu ucggggagau caacauccgg     3600

cgcaucaagg aggugucccu caaggaggcc augaagcgga ucaaggacau cgccgacaag     3660

gagcugcgca gcaagaucgu cgagcugaug gcccuccagu acaacgagaa gaagaucaag     3720

caguacuuca gcgacaacaa ggacgagugg uccgacguga accccaagaa gaucaagauc     3780

cacuacuuca ccaaggagac gaacgagcgg uucuucgcga cccgcaagcc ccuggacacc     3840

agcuucgaca agaagaagau caagacccag guggccgaca cggccaucca gaagauccug     3900

cucaaccacc uguccgccca cggcgacaag gccgagaccg cguucagccc cgacggcauc     3960

gacgagauga accgcaacau caucgagcug aacgacggga aguuccacca gccgauccuc     4020

aagguccgga ucuacgagaa ggccgacaag uacgccgugg gccagcaggg gaacaagaag     4080

aagaaguacg uggaggccgc gaagggcacc aaccuguucu ucgccaucua cgccgaggag     4140

aagaucgacg agaagaccgg ggacacgguc aucacccgca ccuacgacuc caucccccug     4200

aacgugguga ucgaccggga gaagcgcggc cucagccccg uccccgagaa cgagaacggc     4260

gccaagccga aguacgugcu gucccccaac gaccuggugu acguccccaa cgaggaggag     4320

cgggagaccg ggcgcaucug cgagccccuc gacaaggagc ggaucuacaa gauggugagc     4380

uccaacaaga agcaggccuu cuucaucaag agcgaggugg cguccacgau cgucgacaag     4440

uucgaguuca gcgcccugaa caagcaggag cgcgcccugu ccggcgagau gaucaaggag     4500

aucugcaucc cgaucagcau caaccggauc ggggagauca cccuccgcug a              4551


<210>  2276
<211>  4554
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2276
augaagaaga ucgugggccu ggaccucggg accaacagca ucggcugggc ccugaucaac       60

gccuacauca acaaggagca ccuguacggg aucgaggcgu gcggcucccg caucaucccc      120

auggacgccg ccauccucgg gaacuucgac aagggcaaca gcaucuccca gaccgccgac      180

cggacgagcu accgcggcau ccggcgccug cgggagcgcc accuccuccg gcgcgagcgg      240

cugcaccgca uccuggaccu ccugggguuc cugccgaagc acuacuccga cagccucaac      300

cgguacggca aguuccugaa cgacaucgag ugcaagcugc ccugggucaa ggacgagacc      360

ggguccuaca aguucaucuu ccaggagagc uucaaggaga ugcucgccaa cuucaccgag      420

caccacccca uccugaucgc gaacaacaag aaggugcccu acgacuggac caucuacuac      480

cugcgcaaga aggcccucac gcagaagauc uccaaggagg agcuggccug gauccugcuc      540

aacuucaacc agaagcgggg cuacuaccag cugcgcgggg aggaggagga gaccccgaac      600

aagcuggugg aguacuacag ccucaagguc gagaaggugg aggacuccgg cgagcggaag      660

ggcaaggaca ccugguacaa cgugcaccug gagaacggga ugaucuaccg ccggaccagc      720

aacauccccc uggacuggga gggcaagacg aaggaguuca ucgucaccac cgaccucgag      780

gccgacgggu cccccaagaa ggacaaggag ggcaacauca agcgcagcuu ccgggcgccc      840

aaggacgacg acuggacccu gaucaagaag aagacggagg ccgacaucga caagaucaag      900

augaccgugg gcgccuacau cuacgacacc cugcuccaga agccggacca gaagauccgc      960

gggaagcugg ugcggaccau cgagcgcaag uacuacaaga acgagcugua ccagauccuc     1020

aagacgcagu ccgaguucca cgaggagcug cgcgacaagc agcuguacau cgccugccuc     1080

aacgagcugu accccaacaa cgagccccgg cgcaacagca ucuccacccg ggacuucugc     1140

caccuguuca ucgaggacau caucuucuac cagcgccccc ucaagagcaa gaagucccug     1200

aucgacaacu gcccguacga ggagaaccgg uacaucgaca aggagagcgg cgagaucaag     1260

cacgccagca ucaagugcau cgcgaagucc cacccccugu accaggaguu ccgccucugg     1320

caguucaucg ucaaccugcg gaucuaccgc aaggagaccg acguggacgu gacccaggag     1380

cugcucccca cggaggccga cuacgucacc cuguucgagu ggcugaacga gaagaaggag     1440

aucgaccaga aggccuucuu caaguacccg cccuucgggu ucaagaagac caccagcaac     1500

uaccggugga acuacgugga ggacaagccc uaccccugca acgagacgca cgcccagauc     1560

aucgcccgcc ucggcaaggc gcacaucccc aaggccuucc uguccaagga gaaggaggag     1620

acccuguggc acauccugua cagcaucgag gacaagcagg agaucgagaa ggcccuccac     1680

uccuucgcca acaagaacaa ccugagcgag gaguucaucg agcaguucaa gaacuucccg     1740

cccuucaaga aggaguacgg guccuacagc gccaaggcga ucaagaagcu gcucccccug     1800

augcggaugg gcaaguacug guccaucgag aacaucgaca acggcacccg cauccggauc     1860

aacaagauca ucgacgggga guacgacgag aacauccgcg agcgggugcg ccagaaggcc     1920

aucaaccuga ccgacaucac gcacuuccgg gcccuccccc uguggcuggc cugcuaccuc     1980

gucuacgacc gccacagcga ggugaaggac aucgugaagu ggaagacccc gaaggacauc     2040

gaccuguacc ugaaguccuu caagcagcac agccuccgga accccaucgu cgagcagguc     2100

aucaccgaga cccugcgcac ggugcgcgac aucuggcagc aggucggcca caucgacgag     2160

auccacaucg agcuggggcg ggagaugaag aaccccgccg acaagcgcgc gcggaugucc     2220

cagcagauga ucaagaacga gaacaccaac cuccgcauca aggcccugcu gaccgaguuc     2280

cucaaccccg aguucggcau cgagaacgug cggccguaca gccccuccca gcaggaccug     2340

cugcgcaucu acgaggaggg ggugcucaac agcauccugg agcugcccga ggacaucggc     2400

aucauccucg gcaaguucaa ccagaccgac acgcugaagc ggccgacccg cuccgagauc     2460

cugcgguaca agcucuggcu ggagcagaag uaccgcagcc ccuacaccgg ggagaugauc     2520

ccccugucca agcuguucac ccccgccuac gagaucgagc acaucauccc gcagagccgg     2580

uacuucgacg acagccuguc caacaaggug aucugcgaga gcgagaucaa caagcugaag     2640

gaccgcuccc ucggcuacga guucaucaag aaccaccacg gggagaaggu ggagcuggcc     2700

uucgacaagc ccgucgaggu gcugagcgug gaggcguacg agaagcucgu ccacgagucc     2760

uacagccaca accgguccaa gaugaagaag cugcugaugg aggacauccc cgaccaguuc     2820

aucgagcgcc agcucaacga cagccgguac aucuccaagg uggucaagag ccugcugucc     2880

aacaucgugc gcgaggagaa cgagcaggag gccaucagca agaacgugau ccccugcacc     2940

ggcgggauca cggaccggcu caagaaggac uggggcauca acgacgugug gaacaagauc     3000

guccugccgc gcuucauccg gcugaacgag cucaccgagu ccacccgcuu caccagcauc     3060

aacacgaaca acaccaugau ccccuccaug ccccuggagc ugcagaaggg cuucaacaag     3120

aagcggaucg accaccgcca ccacgccaug gacgccauca ucaucgccug cgcgaaccgc     3180

aacaucguga acuaccucaa caacgugagc gccuccaaga acaccaagau cacccggcgc     3240

gaccugcaga cgcugcucug ccacaaggac aagaccgaca acaacgggaa cuacaagugg     3300

gucaucgaca agcccuggga gaccuucacc caggacacgc ugaccgcccu gcagaagauc     3360

accgugagcu ucaagcagaa ccuccgggug aucaacaaga ccacgaacca cuaccagcac     3420

uacgagaacg gcaagaagau cgucagcaac caguccaagg gggacagcug ggccauccgc     3480

aaguccaugc acaaggagac cgugcacggc gaggugaacc ugcggaugau caagaccguc     3540

agcuucaacg aggcccugaa gaagccgcag gcgaucgugg agauggaccu caagaagaag     3600

auccuggcca ugcuggagcu cggcuacgac accaagcgca ucaagaacua cuucgaggag     3660

aacaaggaca cguggcagga caucaacccc uccaagauca agguguacua cuucaccaag     3720

gagaccaagg accgguacuu cgccguccgc aagcccaucg acaccagcuu cgacaagaag     3780

aagaucaagg aguccaucac ggacaccggg auccagcaga ucaugcugcg gcaccuggag     3840

accaaggaca acgaccccac ccucgccuuc agcccggacg gcaucgacga gaugaaccgc     3900

aacauccuga uccugaacaa ggggaagaag caccagccca ucuacaaggu gcggguguac     3960

gagaaggccg agaaguucac ggucggccag aaggggaaca agcgcaccaa guucguggag     4020

gcggccaagg gcaccaaccu cuucuucgcc aucuacgaga ccgaggagau cgacaaggac     4080

acgaagaagg ugauccggaa gcgcuccuac agcaccaucc cccugaacgu cgugaucgag     4140

cggcagaagc agggccuguc cagcgccccc gaggacgaga acgggaaccu cccgaaguac     4200

auccuguccc ccaacgaccu gguguacguc cccacccagg aggagaucaa caagggcgag     4260

guggugaugc ccaucgaccg cgaccggauc uacaagaugg ucgacagcuc cgggaucacc     4320

gcgaacuuca ucccggccag cacggccaac cucaucuucg cccugcccaa ggccaccgcg     4380

gagaucuacu gcaacggcga gaacugcauc cagaacgagu acgggaucgg cagcccccag     4440

uccaagaacc agaaggccau caccggcgag auggugaagg agaucugcuu cccgaucaag     4500

guggaccgcc uggggaacau cauccagguc ggcuccugca uccucaccaa cuga           4554


<210>  2277
<211>  4563
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2277
augaagaaga ucgugggccu ggaccucggg accaacagca ucggcugggc ccugaucaac       60

gccuacaucg acgaguacaa caaggagcac cuguacggga ucgaggcgug cggcucccgc      120

aucaucccca uggacgccgc cauccucggg aacuucgaca agggcaacag caucucccag      180

accgccgacc ggacgagcua ccgcggcauc cggcgccugc gggagcgcca ccuccuccgg      240

cgcgagcggc ugcaccgcau ccuggaccuc cugggguucc ugccgaagca cuacuccgac      300

agccucaacc gguacggcaa guuccugaac gacaucgagu gcaagcugcc cugggucaag      360

gacgagaccg gguccuacaa guucaucuuc caggagagcu ucaaggagau gcucgccaac      420

uucaccgagc accaccccau ccugaucgcg aacaacaaga aggugcccua cgacuggacc      480

aucuacuacc ugcgcaagaa ggcccucacg cagaagaucu ccaaggagga gcuggccugg      540

auccugcuca acuucaacca gaagcggggc uacuaccagc ugcgcgggga ggaggaggag      600

accccgaaca agcuggugga guacuacagc cucaaggucg agaaggugga ggacuccggc      660

gagcggaagg gcaaggacac cugguacaac gugcaccugg agaacgggau gaucuaccgc      720

cggaccagca acaucccccu ggacugggag ggcaagacga aggaguucau cgucaccacc      780

gaccucgagg ccgacggguc ccccaagaag gacaaggagg gcaacaucaa gcgcagcuuc      840

cgggcgccca aggacgacga cuggacccug aucaagaaga agacggaggc cgacaucgac      900

aagaucaaga ugaccguggg cgccuacauc uacgacaccc ugcuccagaa gccggaccag      960

aagauccgcg ggaagcuggu gcggaccauc gagcgcaagu acuacaagaa cgagcuguac     1020

cagauccuca agacgcaguc cgaguuccac gaggagcugc gcgacaagca gcuguacauc     1080

gccugccuca acgagcugua ccccaacaac gagccccggc gcaacagcau cuccacccgg     1140

gacuucugcc accuguucau cgaggacauc aucuucuacc agcgcccccu caagagcaag     1200

aagucccuga ucgacaacug cccguacgag gagaaccggu acaucgacaa ggagagcggc     1260

gagaucaagc acgccagcau caagugcauc gcgaaguccc acccccugua ccaggaguuc     1320

cgccucuggc aguucaucgu caaccugcgg aucuaccgca aggagaccga cguggacgug     1380

acccaggagc ugcuccccac ggaggccgac uacgucaccc uguucgagug gcugaacgag     1440

aagaaggaga ucgaccagaa ggccuucuuc aaguacccgc ccuucggguu caagaagacc     1500

accagcaacu accgguggaa cuacguggag gacaagcccu accccugcaa cgagacgcac     1560

gcccagauca ucgcccgccu cggcaaggcg cacaucccca aggccuuccu guccaaggag     1620

aaggaggaga cccuguggca cauccuguac agcaucgagg acaagcagga gaucgagaag     1680

gcccuccacu ccuucgccaa caagaacaac cugagcgagg aguucaucga gcaguucaag     1740

aacuucccgc ccuucaagaa ggaguacggg uccuacagcg ccaaggcgau caagaagcug     1800

cucccccuga ugcggauggg caaguacugg uccaucgaga acaucgacaa cggcacccgc     1860

auccggauca acaagaucau cgacggggag uacgacgaga acauccgcga gcgggugcgc     1920

cagaaggcca ucaaccugac cgacaucacg cacuuccggg cccucccccu guggcuggcc     1980

ugcuaccucg ucuacgaccg ccacagcgag gugaaggaca ucgugaagug gaagaucccg     2040

aaggacaucg accuguaccu gaaguccuuc aagcagcaca gccuccggaa ccccaucguc     2100

gagcagguca ucaccgagac ccugcgcacc gugcgcgaca ucuggcagca ggucggccac     2160

aucgacgaga uccacaucga gcuggggcgg gagaugaaga accccgccga caagcgcgcg     2220

cggauguccc agcagaugau caagaacgag aacacgaacc uccgcaucaa ggcccugcug     2280

accgaguucc ucaaccccga guucggcauc gagaacgugc ggccguacag ccccucccag     2340

caggaccugc ugcgcaucua cgaggagggg gugcucaaca gcauccugga gcugcccgag     2400

gacaucggca ucauccucgg caaguucaac cagaccgaca cccugaagcg gccgacgcgc     2460

uccgagaucc ugcgguacaa gcucuggcug gagcagaagu accgcagccc cuacaccggg     2520

gagaugaucc cccuguccaa gcuguucacc cccgccuacg agaucgagca caucaucccg     2580

cagagccggu acuucgacga cagccugucc aacaagguga ucugcgagag cgagaucaac     2640

aagcugaagg accgcucccu cggcuacgag uucaucaaga accaccacgg ggagaaggug     2700

gagcuggccu ucgacaagcc cgucgaggug cugagcgugg aggcguacga gaagcucguc     2760

cacgaguccu acagccacaa ccgguccaag augaagaagc ugcugaugga ggacaucccc     2820

gaccaguuca ucgagcgcca gcucaacgac agccgguaca ucuccaaggu ggucaagagc     2880

cugcugucca acaucgugcg cgaggagaac gagcaggagg ccaucagcaa gaacgugauc     2940

cccugcaccg gcgggaucac cgaccggcuc aagaaggacu ggggcaucaa cgacgugugg     3000

aacaagaucg uccugccgcg cuucauccgg cugaacgagc ucacggaguc cacccgcuuc     3060

accagcauca acaccaacaa cacgaugauc cccuccaugc cccuggagcu gcagaagggc     3120

uucaacaaga agcggaucga ccaccgccac cacgccaugg acgccaucau caucgccugc     3180

gcgaaccgca acaucgugaa cuaccucaac aacgugagcg ccuccaagaa caccaagauc     3240

acccggcgcg accugcagac ccugcucugc cacaaggaca agacggacaa caacgggaac     3300

uacaaguggg ucaucgacaa gcccugggag accuucaccc aggacacccu gacggcccug     3360

cagaagauca ccgugagcuu caagcagaac cuccggguga ucaacaagac caccaaccac     3420

uaccagcacu acgagaacgg caagaagauc gucagcaacc aguccaaggg ggacagcugg     3480

gccauccgca aguccaugca caaggagacg gugcacggcg aggugaaccu gcggaugauc     3540

aagaccguca gcuucaacga ggcccugaag aagccgcacg cgaucgugga gauggaccuc     3600

aagaagaaga uccuggccau gcuggagcuc ggcuacgaca ccaagcgcau caagaacuac     3660

uucgaggaga acaaggacac cuggcaggac aucaaccccu ccaagaucaa gguguacuac     3720

uucacgaagg agaccaagga ccgguacuuc gccguccgca agcccaucga caccagcuuc     3780

gacaagaaga agaucaagga guccaucacc gacacgggga uccagcagau caugcugcgg     3840

caccuggaga ccaaggacaa cgaccccacc cucgccuuca gcccggacgg caucgacgag     3900

augaaccgca acauccugau ccugaacaag gggaagaagc accagcccau cuacaaggug     3960

cggguguacg agaaggccga gaaguucacc gucggccaga aggggaacaa gcgcacgaag     4020

uucguggagg cggccaaggg caccaaccuc uucuucgcca ucuacgagac cgaggagauc     4080

gacaaggaca ccaagaaggu gauccggaag cgcuccuaca gcacgauccc ccugaacguc     4140

gugaucgagc ggcagaagca gggccugucc agcgcccccg aggacgagaa cgggaaccuc     4200

ccgaaguaca uccugucccc caacgaccug guguacgucc ccacccagga ggagaucaac     4260

aagggcgagg uggugaugcc caucgaccgc gaccggaucu acaagauggu cgacagcucc     4320

gggaucaccg cgaacuucau cccggccagc accgccaacc ucaucuucgc ccugcccaag     4380

gccacggcgg agaucuacug caacggcgag aacugcaucc agaacgagua cgggaucggc     4440

agcccccagu ccaagaacca gaaggccauc accggcgaga uggugaagga gaucugcuuc     4500

ccgaucaagg uggaccgccu ggggaacauc auccaggucg gcuccugcau ccucaccaac     4560

uga                                                                   4563


<210>  2278
<211>  4563
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2278
augaagaaga ucgugggccu ggaccucggg accaacagca ucggcugggc ccugaucaac       60

gccuacaucg acgaguacaa caaggagcac cuguacggga ucgaggcgug cggcucccgc      120

aucaucccca uggacgccgc cauccucggg aacuucgaca agggcaacag caucucccag      180

accgccgacc ggacgagcua ccgcggcauc cggcgccugc gggagcgcca ccuccuccgg      240

cgcgagcggc ugcaccgcau ccuggaccuc cugggguucc ugccgaagca cuacuccgac      300

agccucaacc gguacggcaa guuccugaac gacaucgagu gcaagcugcc cugggucaag      360

gacgagaccg gguccuacaa guucaucuuc caggagagcu ucaaggagau gcucgccaac      420

uucaccgagc accaccccau ccugaucgcg aacaacaaga aggugcccua cgacuggacc      480

aucuacuacc ugcgcaagaa ggcccucacg cagaagaucu ccaaggagga gcuggccugg      540

auccugcuca acuucaacca gaagcggggc uacuaccagc ugcgcgggga ggaggaggag      600

accccgaaca agcuggugga guacuacagc cucaaggucg agaaggugga ggacuccggc      660

gagaagaagg gcaaggacac cugguacaac gugcaccugg agaacgggau gaucuaccgg      720

cgcaccagca acaucccccu ggacugggag ggcaagacga aggaguucau cgucaccacc      780

gaccucgagg ccgacggguc ccccaagaag gacaaggagg gcaacaucaa gcggagcuuc      840

cgcgcgccca aggacgacga cuggacccug aucaagaaga agacggaggc cgacaucgac      900

aagaucaaga ugaccguggg cgccuacauc uacgacaccc ugcuccagaa gccggaccag      960

aagauccggg ggaagcuggu gcgcaccauc gagcggaagu acuacaagaa cgagcuguac     1020

cagauccuca agacgcaguc cgaguuccac gaggagcugc gcgacaagca gcuguacauc     1080

gccugccuca acgagcugua ccccaacaac gagccccgcc ggaacagcau cuccacccgc     1140

gacuucugcc accuguucau cgaggacauc aucuucuacc agcggccccu caagagcaag     1200

aagucccuga ucgacaacug cccguacgag gagaaccgcu acaucgacaa ggagagcggc     1260

gagaucaagc acgccagcau caagugcauc gcgaaguccc acccccugua ccaggaguuc     1320

cggcucuggc aguucaucgu caaccugcgc aucuaccgga aggagaccga cguggacgug     1380

acccaggagc ugcuccccac ggaggccgac uacgucaccc uguucgagug gcugaacgag     1440

aagaaggaga ucgaccagaa ggccuucuuc aaguacccgc ccuucggguu caagaagacc     1500

accagcaacu accgcuggaa cuacguggag gacaagcccu accccugcaa cgagacgcac     1560

gcccagauca ucacccggcu cggcaaggcc cacaucccca aggcguuccu guccaaggag     1620

aaggaggaga cccuguggca cauccuguac agcaucgagg acaagcagga gaucgagaag     1680

gcccuccacu ccuucgccaa caagaacaac cugagcgagg aguucaucga gcaguucaag     1740

aacuucccgc ccuucaagaa ggaguacggg uccuacagcg ccaaggccau caagaagcug     1800

cucccccuga ugcgcauggg caaguacugg uccaucaaga acaucgacaa cggcacccgg     1860

auccgcauca acaagaucau cgacggggag uacgacgaga acauccggga gcgcgugcgg     1920

cagaaggcga ucaaccugac ggacaucacc cacuuccgcg cccucccccu guggcuggcc     1980

ugcuaccucg ucuacgaccg gcacagcgag gugaaggaca ucgugaagug gaagaccccg     2040

aaggacaucg accuguaccu gaaguccuuc aagcagcaca gccuccgcaa ccccaucguc     2100

gagcagguca ucaccgagac gcugcggacc gugcgcgaca ucuggcagca ggucggccac     2160

aucgacgaga uccacaucga gcuggggcgc gagaugaagu cccccgccga caagcgggcc     2220

cgcaugagcc agcagaugau caagaacgag aacaccaacc uccggaucaa ggcgcugcug     2280

accgaguucc ucaaccccga guucggcauc gagaacgugc gcccguacuc ccccagccag     2340

caggaccugc ugcggaucua cgaggagggg gugcucaacu ccauccugga gcugcccgag     2400

gacaucggca ucauccucgg caaguucaac cagacggaca cccugaagcg cccgacccgg     2460

agcgagaucc ugcgcuacaa gcucuggcug gagcagaagu accggucccc cuacaccggg     2520

gagaugaucc cccugagcaa gcuguucacc cccgccuacg agaucgagca caucaucccg     2580

cagagccgcu acuucgacga cucccugagc aacaagguga ucugcgaguc cgagaucaac     2640

aagcugaagg accggagccu cggcuacgag uucaucaaga accaccacgg ggagaaggug     2700

gagcuggccu ucgacaagcc cgucgaggug cuguccgugg aggccuacga gaagcucguc     2760

cacgagagcu acucccacaa ccgcagcaag augaagaagc ugcugaugga ggacaucccc     2820

gaccaguuca ucgagcggca gcucaacgac ucccgcuaca ucagcaaggu ggugaagucc     2880

cugcugagca acaucguccg ggaggagaac gagcaggagg cgaucuccaa gaacgugauc     2940

cccugcacgg gcgggaucac cgaccgccuc aagaaggacu ggggcaucaa cgacgugugg     3000

aacaagaucg uccugccgcg guucauccgc cugaacgagc ucaccgagag cacccgguuc     3060

acguccauca acaccaacaa caccaugauc cccagcaugc cccuggagcu gcagaagggc     3120

uucaacaaga agcgcaucga ccaccggcac cacgccaugg acgccaucau caucgccugc     3180

gccaaccgca acaucgugaa cuaccucaac aacguguccg cgagcaagaa caccaagauc     3240

acgcgccggg accugcagac ccugcucugc cacaaggaca agaccgacaa caacgggaac     3300

uacaaguggg ucaucgacaa gcccugggag accuucacgc aggacacccu gaccgcccug     3360

cagaagauca ccgugagcuu caagcagaac cuccgcguga ucaacaagac gaccaaccac     3420

uaccagcacu acgagaacgg caagaagauc gucuccaacc agagcaaggg ggacuccugg     3480

gccauccgca agagcaugca caaggagacc gugcacggcg aggugaaccu gcggaugauc     3540

aagaccgucu cccugaacga ggcccucaag aagccgcagg ccaucgugga gauggaccug     3600

aagaagaaga uccuggcgau gcucgagcug ggcuacgaca cgaagcggau caagaacuac     3660

uucgaggaga acaaggacac cuggcaggac aucaacccca gcaagaucaa gguguacuac     3720

uucaccaagg agaccaagga ccgcuacuuc gccguccgga agcccaucga cacguccuuc     3780

gacaagaaga agaucaagga gagcaucacc gacaccggga uccagcagau caugcugcgc     3840

caccucgaga ccaaggacaa cgaccccacg cuggccuucu ccccggacgg caucgacgag     3900

augaaccgga acauccugau ccucaacaag gggaagaagc accagcccau cuacaaggug     3960

cgcguguacg agaaggccga gaaguucacc gucggccaga aggggaacaa gcggaccaag     4020

uucguggagg ccgcgaaggg caccaaccug uucuucgcca ucuacgagac ggaggagauc     4080

gacaaggaca ccaagaaggu gauccgcaag cggagcuacu ccaccauccc ccugaacguc     4140

gugaucgagc gccagaagca gggccucagc uccgcccccg aggacgagaa cgggaaccug     4200

ccgaaguaca uccugagccc caacgaccuc guguacgucc ccacccagga ggagaucaac     4260

aagggcgagg uggugaugcc caucgaccgg gaccgcaucu acaagauggu cgacuccagc     4320

gggaucacgg ccaacuucau cccggcgucc accgccaacc ugaucuucgc ccugcccaag     4380

gccaccgccg agaucuacug caacggcgag aacugcaucc agaacgagua cgggaucggc     4440

agcccccagu ccaagaacca gaaggcgauc accggcgaga uggugaagga gaucugcuuc     4500

ccgaucaagg uggaccggcu cgggaacauc auccaggucg gcagcugcau ccugaccaac     4560

uga                                                                   4563


<210>  2279
<211>  4575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2279
augaacaaga uccugggccu cgaccugggg accaacagca ucggcugggc caucgugcag       60

aaggacgagc agggguucua cgagaagauc aucaacgccg gcucccgcau cauccccaug      120

gacgcggaga ccaugaacaa cuucaacaag gggaucacca agagcccggc cuccgagcgg      180

acgcgcuucc ggggcgcccg ccggcugauc gagcgcagcc uccugcggcg cgagcggauc      240

caccgccugc ucaacaccau gaacuaccug ccgccccacu acgccgagaa gaucgacuuc      300

gucaaccggc ugggcaaguu caucggggag gaggagccca aguacgccua caagguggac      360

gagcacggca aguuccaguu caucuucaag gaguccuuca acgagaugcu caaggacuuc      420

caggacaagc agcccgagcu ggugcugaac aacaagaagg uccccuacga cuggaccauc      480

uacuaccucc gcaagaaggc gcugaaccgg gccaucagca aggaggagcu gggguggauc      540

auccuccagu ucaacaacaa gcgcggcuac uaccagcugc gcggggagga ggacgagacg      600

cugaccgagg gccagaagaa ggaguacuuc gcccucaagg ugauccgggu ggaggccgac      660

uccagcuccg cgggcaagaa gggggagguc ugguacaacg ugcagcugga gaacggcugg      720

aucuaccggu gcaagagcga gaucccccug gacugggagg ggaagaucaa ggaguucauc      780

gugaccaccg aggucgacga gaacgacaac gugcucaagg acaaggaggg caaggagaag      840

cgcaagcuga agauccccga ggccggcgac uggacgcugc ucaagaagaa gaccgagucc      900

gaccuggaca agagcgggaa gaccgugggc gccuacaucu acgacacccu gcucuccaac      960

ccggaccaga agaucaaggg cgggcugauc cggacgaucg agcgcgagcu guaccggagc     1020

gagcucgagc agauccugga caagcagaag gaguuccacc ccgagcugaa ggacgggacc     1080

cucuacaaca agugcgucga ggagcuguac cgcuccaacg agagccgccg gaucucccug     1140

agcaacuacg acuucacccg cuucaucacc gaggacguga ucuucuacca gcggccccuc     1200

aaguccaaga agucccugau cgacaacugc agcuucgagc gccggaacua ccugaacccc     1260

aucacgaagg agcucgccua cgccccgauc aagugcaucg ccaagagcca cccccuguuc     1320

caggaguucc gccuguggca guucaucaag aaccuccgga ucaucgagcg cgagaagauc     1380

gugggcgaca agcugauguu cgaccaggac gucaccaccg aguuccugcc cacccagaac     1440

gacuacguga uccucuucga cuggcugaac gagcacaagg agaucgacca gaaggcgcug     1500

cucaaguacc ccgccuucaa ccugaagaag aacaucgaca aguaccggug gaacuacgug     1560

gagaacaaga gcuacccgug caacgagacg cgcgcccugc ugaaggccaa gcucaacaag     1620

gcgggcaaca uccccuccga cuuccuggac aacgacaccc uggagagccu cuggcacauc     1680

cuguacuccg ucgaggacaa guucgagauc gagaaggccc ucaagagcuu cgccaccaag     1740

aaggagcugc gggaggagca gaccguggcc uucgugaagc aguucaucaa caucccgccc     1800

uucaagaagg aguacggguc cuacagcgcc aaggcgauca agaagcugcu gcagcucaug     1860

cgcaugggcu cccgguggag cgagaacgag aucuccgaca gcacgaagga ccgcauccag     1920

aagcugaucg acggggagug ggacgagucc aucaagaccc ggacccgcga caaggccauc     1980

aagcugaacg agaucaacga cuuccagggc cucccccugu ggcuggccag cuacaucguc     2040

uacgaccggc acuccgagag cggggacgug augaaguggg agacccccga ggacaucgac     2100

uacuaccuca agcacguguu caagcagcac ggccugcgca accccaucgu cgaggugguc     2160

gugguggaga cgcugaaggu cgugaaggac cucuggaaga ccuacggcua cuucaacgag     2220

auccacaucg agcuggggcg ggagaugaag aaccccgccg acaagcgccg cuccaugacc     2280

aacaagaaca ucgagaacga gaacaccaac cugcggauca aggcccuccu ggcggagcug     2340

gccaaccaca aggacgugga gggcguccgc ccguacagcc ccucccagca ggagauccuc     2400

aagaucuacg aggagggggu gcuggacaug cugacgaagg aggaccccga cuacgagagc     2460

aucagcaaga ucuccaagau ggcccucccc agcuccagcg agcugauccg guacaagugc     2520

uggcuggagc agaaguaccg cuccccguac accggccagg ugaucccccu cgcccggcug     2580

uucaccagcg aguacgagau cgagcacauc aucccccagu cccgcuacuu cgacgacagc     2640

uucuccaaca aggucaucug cgagagcgcg gugaacaagg acaaggacaa ccugcucgcc     2700

uacgaguaca ucaagcagaa cgaggggaag aucaucgaga ucggccuggg caagaaggug     2760

aagcuguuca ccgccgacuc cuacacggag uucguccaga gcaacuacgu gggguccgug     2820

gccaagaaga agaagcuccu gauggacacc aucccggaca gcuucaucaa gcggcagcug     2880

aacgacuccc gcuacaucag ccgggagauc augaagcucc uguccagcgu cgugcgcgag     2940

aaggaggagg aggaggagga ggaggaggag gaggccauca gcaagaacgu gaucgucugc     3000

accggcgcga ucaccgacaa gcugaagcgg gagugggggc ucaacgacgu guggaacacc     3060

aucaucuacc cccgcuucga gcggcugaac cagcugacga acuccgacaa guucggccag     3120

ugggagaaca agcagggcaa gaagguguuc cagaucgaga ugccccucaa gcugcagaag     3180

ggguucaaca agaagcgcau cgaccaccgg caccacgcca uggacgccau cgucaucgcc     3240

ugcgccaccc agagccacgu gaacuaccug aacaacgagu ccgcgcacag caacuccaag     3300

gagaagcgcu acuaccuccg gcgcaagcug cggcgcaucg agauccugga gaagcaggag     3360

cucaaggacg gcgugaccau caccaagaag aucgaggucg ccaaggaguu caacaagccc     3420

uggccgacgu ucacccagga cgcccacgag gugcugcaga gcaucaucgu guccuucaag     3480

cagaaccugc gggucgugaa caaggccacc aaccgcuacg ccugcuucgu gaacgggaag     3540

aaggagaucg ucaagcagag caagggcgag uccugggcga uccgcaagcc caugcacaag     3600

gacaccguga gcgccgccgu gucccuccgg aagaucaaga cggucagccu gucccaggcc     3660

aucgacgacu ggaccaacau cguggacaag agccugcgca aggagaucgg gcuccuguac     3720

uccaaguacg gccagaacgg cagcaagaac aucaucaagu acuucaagga ccgggacaac     3780

aagcacaacg ggcuggacgu guccaagguc aacgugcacu acuucgacaa cgacuacgcg     3840

gccagccgcg uggcccucga ccacaaguuc aacuccgcca agaucaagag caucaccgac     3900

accggcaucc agacgauccu gcugaagcac cucgagagcu acaacgagau caacggggac     3960

aagaucaucg agcaccccga gcuggccuuc uccgcggacg gccuggacau ccucaacgcc     4020

aacaucacca agcugaacaa cgggaaguuc cacaagccca ucaagaaggu ccggaagacc     4080

gagacccugg gccucaaguu caucgugggc cagacgggga acaagcgcaa gaaguacgug     4140

gaggccgccg acggcaccaa ccuguucuuc gccaucuacg ucagcgugga cgggauccgg     4200

uccuacaaga ccaucccgcu gcgcgaggug gcggagcggc ucaaggaggg ccugaucccc     4260

guccccgaga agaacgacaa ggacgaccac cuccuguucu ggcugagccc cggggaccug     4320

guguacgugc cguccaucga ggaggagggc cgcaucgucg agaucgagaa caucaagcag     4380

gaccggaucu acaagguggu gagcugcacc gagaacgagu gccacugcau cccccacuuc     4440

gucgccuccc ccaucgugaa gacggacgag cucggcgcca acaacaaggc ccagcgcgcc     4500

uggagcgggg agaugaucaa ggagaccugc aucaagauca agaugaaccg gcugggccag     4560

aucgucaaga ucuga                                                      4575


<210>  2280
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2280
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cgugcugaac       60

gccgcggacg acaacucccu caaguacauc gaggccgccg ggagccgcau cauccccaug      120

gacgccgcca uccugggcga cuucgaccgg gggaacucca ucagccagac cgcggagcgc      180

acgcggaucc gcggcaugcg gcaccuccgc gagcgguacc uccugcgccg ggcccgccug      240

caccggaucc ucaaccugcu gggcuuccuc ccguuccacu acgcccagga caucgaccgc      300

uacgggaacu ucuccgccga cauggagccc aagaucgcgu ggcggaagaa ccuggagggc      360

gagcuggagu ucaucuucau cgagagcuuc aacgagaugc ucgaggacuu ccgcaagaac      420

cagcccgagc uggucgccga caacaagaag gugcccuacg acugggugau cuacuaccug      480

cggaagaagg cccucgagaa gaagaucucc aaggaggagc uggccuggau ccugcucaac      540

uucaaccaga agcgcgggua cuaccagcug cggggcgagg acgacgacga gagcaugaag      600

accgccaaga cccgccagua cuucgacaag cagaucauca agaacaucau cgacaccaac      660

cagacguaca aggggcagcg gauccugauc gucgagcucc agaacggcga caagggcaag      720

aucuucaaga aggagauccc ggacuggauc gggcaggaga agaacaucau cgugaccgug      780

gacaucgaca aggacggcaa cgacaaguac gaggaguccg ggagccuguc cugccgcuuc      840

aagaucccca ccgacgcgga gugggagagc gaguggaagc ugaucaagau gaagacccag      900

aaggaccucg acgaguccaa caagacgguc ggcaccuaca ucuacgacag cauccugcag      960

aaccccaccc agaaggugcg gggcaagcug gugcgcacca ucgagcgcaa guacuacaag     1020

gccgagcuca agcagauccu ggagaagcag acggaguucc acuccgagcu gcaggacaag     1080

gaccucuaca ucgagugccu gaacgagcug uacagcaaca acgacaccca ccggaacgcc     1140

aucaccgacc gcgacuucgu cuaccucuuc augaacgaca uccuguucua ccagcggccc     1200

cugaagucca agaagucccu caucgacaac ugcccguacg agcgcaacau cuacaucgac     1260

aaggagaccg gggagaucaa guacuccccc aucaagugca ucgccaagag ccacccccug     1320

uuccaggagu uccggcugug gaaguucauc agcuccaucc gcaucuacca gcgggagagc     1380

gugguggagg ucguggacaa gcagcucgac cuguucucca gccaguccac gagcgugcag     1440

aagcugguca ccgacgugga cgugaccgac aaguuccuga ccugcgagaa cgacuacguc     1500

gcccuguucg aguggcugaa caagaagaag aucaucgacc agaaggaguu ccucaaguac     1560

cgccccuucg gccugaaggg ggacaucgac aacuaccggu ggaacuacgu ggaggacaag     1620

uccuacccgu gcaacgagac gcacgcgaug auccugaccu acuucgccaa ggccggcauc     1680

agcgaggaca uccugaccaa ggagauggag gaggcccucu ggcacauccu guacuccgug     1740

gaggaccgcc aggagaucac caaggcccug cggacguucg cgagcaagua ccaccucucc     1800

gacagcuucg ucaagguguu cuccaacauc ccgcccuuca agaaggagua cgggagcuac     1860

uccgccaagg ccaucaagaa gcugcugagc cucaugcgca ugggcaagua cugguccgag     1920

gacgccaucg acgugaacac ccgggagcgc aucgacaaga ucgucaccgg cgaguacgac     1980

gagaagaucc gggaccgcgu gcgggagaag accauccgcc ugaacgacau ccggcacuuc     2040

cgcgcccugc ccgacuggcu cgcgugcuac gucguguacg accgccacag cgagacgcgg     2100

gagaucaccg aguggaaguc ccccgaggag aucgacgagu acauccgcaa cuucaagcag     2160

cacagccugc ggaaccccau cguggagcag gucaucaccg agacccugcg cacggugcgg     2220

gacaucugga agcaggucgg gugcaucgac gagauccacg uggagcucgg ccgcgagaug     2280

aagaaccccg ccgacaagcg gaagcgcauc accagccaga ucaucgagaa cgagaacgcc     2340

aaccugcggg ugaaggcccu gcucaccgag uucgugaacc ccgaguucga gaucgagaac     2400

guccgcccgu acucccuggg gcagcaggac auccugcgga ucuacgagga cggcgugcuc     2460

aacagcgugg cggagguccc cggggacauc gccggcaucc ugaagaaguu caacgagaac     2520

gacgccaaga agcgccccac cacguccgag auccugcggu acaagcucug gcuggagcag     2580

aaguaccgca gccccuacac cggcgagaug aucccgcugu ccaagcuguu caccagcgcc     2640

uacgagaucg agcacgugau cccccagucc cgguacuucg acgacagcuu cuccaacaag     2700

gugaucugcg aggccgaggu caacaagcuc aaggacaacg cgcuggggua cgaguucauc     2760

aagaagcacc acggcgagaa gguggagcuc ggguucggca agaucgugga gaucuucagc     2820

gucuccgugu acgagcaguu cgugaaggac aacuacagcc gcaaccgggg gaagaugaag     2880

aagcugcuga uggacgagau ccccgagcag uucaucgagc gccagcucaa cgacucccgg     2940

uacaucagca aggucgugaa gacccugcug uccaacgugg uccgcgagaa ggccgagcag     3000

gaggccguga gcaagaacgu gaucgucugc accggcggcg ugacggaccg gcucaagaag     3060

gacuggggga ucaacgacgu guggaacaag aucguccugc cccgcuuccu gcgccucaac     3120

gagcugacca acuccaccaa cuucaccgcc aagaacgccg cgggcaacga gaucccggcc     3180

augccccugg agcuccagaa gggguucaac aagaagcgga ucgaccaccg ccaccacgcc     3240

auggacgcca ucgugaucgc cugcgcgaac cggaacaucg ugaacuaccu gaacaacgag     3300

agcgccagcc gcaaggccaa gaucucccgg cacgaccugc aggccauccu cugcgacaag     3360

guccgcacgg acgacaaggg caacuaccag uggcggguga agaagcccug ggacaccuuc     3420

acccaggacg uguacgucgc gcuggagaac aucaucguga gcuucaagca gaaccugcgc     3480

gugaucaaca agaccuccaa cuacuacugg cacuacgaga acggcaagaa gguccucgug     3540

aagcagguca agggggacag cugggccauc cggaagccga ugcacaagga cacgguguuc     3600

ggcgagguga accugcgcaa ggugaagucc gucagccuga acgaggcccu caagaacccc     3660

caguucaucg uggagaagga ccugaagcgg cagcugcucu cccugcugca gcagaacuuc     3720

gacaucaaga agaucaagaa guacuucgag gagcacaagg acaucuggag cgacaucucc     3780

cucagcaaga ugaacaucua cuacuucacc aaggagacca aggaccgcua cuucgccacc     3840

cggaagcccc uggacgccuc cuucgacaag aagaagaucg agagcugcau cacggacacc     3900

gggauccagc agauccugcu ccgccaccug gagcugaagg agaacgaccc cgagcgggcg     3960

uucuccccgg acggcaucga cgagaugaac cgcaacauca uccagcucaa caaggggaag     4020

ggccaccacc ccaucgccaa ggugcggguc uacgagaagg ccgacaaguu cgaggugggc     4080

cagcgcggga acaagagcuc caaguucgug gaggccgcca agggcaccaa ccuguucuuc     4140

gcggucuacg agaccgagua ccccgacgag aagacgaaca gcaucgugcg gaagcgcaac     4200

uacgccacca ucccccugaa caucgccauc gagcgccaga agcgggggcu cuccaccgcc     4260

ccggaggacg agaagggcaa cguccccagc uucgugcugu cccccaacga ccugguguac     4320

cuccccacca gcgaggacau cgucaacggg cacaucacgc agccgaucga caagagccgc     4380

aucuacaaga ugguguccag cuccggcagc cagugcuucu ucaucaagca caacaucgcc     4440

aacaucaucg uggacaagua cgaguucagc ccccugaaca agauggagcg ggcgaucagc     4500

aacgagauga ucaaggagau cugcaucccc gucaaggugg accgccuggg caacaucgug     4560

aagcucggga aguccgagug a                                               4581


<210>  2281
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2281
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cgugcugaac       60

gccgcggacg acaacucccu caaguacauc gaggccgccg ggagccgcau cauccccaug      120

gacgccgcca uccugggcga cuucgaccgg gggaacucca ucagccagac cgcggagcgc      180

acgcggaucc gcggcaugcg gcaccuccgc gagcgguacc ugcugcgccg ggcccgccug      240

caccggaucc ucaaccugcu gggcuuccuc ccguuccacu acgcccagga caucgaccgc      300

uacgggaacu ucuccgccga cauggagccc aagaucgcgu ggugcaagaa ccuggagggc      360

gagcuggagu ucaucuucau cgagagcuuc aacgagaugc ucgaggacuu ccggaagaac      420

cagcccgagc uggucgccga caacaagaag gugcccuacg acugggugau cuacuaccug      480

cgcaagaagg cccucgagaa gaagaucucc aaggaggagc uggccuggau ccugcucaac      540

uucaaccaga agcgggggua cuaccagcug cgcggcgagg acgacgacga gagcaugaag      600

accgccaaga cccggcagua cuucgacaag cagaucauca agaacaucau cgacaccaac      660

cagacguaca aggggcagcg cauccugauc gucgagcucc agaacggcga caagggcaag      720

aucuucaaga aggagauccc ggacuggauc gggcaggaga agaacaucau cgugaccgug      780

gacaucgaca aggacggcaa cgacaaguac gaggaguccg ggagccuguc cugccgguuc      840

aagaucccca ccgacgcgga gugggagagc gaguggaagc ugaucaagau gaagacccag      900

aaggaccucg acgaguccaa caagacgguc ggcaccuaca ucuacgacag cauccugcag      960

aaccccaccc agaaggugcg cggcaagcug gugcggacca ucgagcgcaa guacuacaag     1020

gccgagcuca agcagauccu ggagaagcag acggaguucc acuccgagcu gcaggacaag     1080

gaccucuaca ucgagugccu gaacgagcug uacagcaaca acgacaccca ccgcaacgcc     1140

aucaccgacc gggacuucgu cuaccucuuc augaacgaca uccuguucua ccagcgcccc     1200

cugaagucca agaagucccu caucgacaac ugcccguacg agcggaacau cuacaucgac     1260

aaggagaccg gggagaucaa guaccugccc aucaagugca ucgccaaguc ccacccccug     1320

uuccaggagu uccgccucug gaaguucauc agcagcaucc ggaucuacca gcgcgagucc     1380

gugguggagg ucguggacaa gcagcuggac cuguucagcu cccagagcac guccgugcag     1440

aagcucguca ccgacgugga cgugaccgac aaguuccuga ccugcgagaa cgacuacguc     1500

gcccuguucg aguggcucaa caagaagaag aucaucgacc agaaggaguu ccugaaguac     1560

cggcccuucg gccugaaggg ggacaucgac aacuaccgcu ggaacuacgu ggaggacaag     1620

agcuacccgu gcaacgagac gcacgcgaug auccugaccu acuucgccaa ggccggcauc     1680

uccgaggaca uccucaccaa ggagauggag gaggcccugu ggcacauccu guacagcgug     1740

gaggaccggc aggagaucac caaggcccuc cgcacguucg cguccaagua ccaccugagc     1800

gacuccuucg ucaagguguu cagcaacauc ccgcccuuca agaaggagua cggguccuac     1860

agcgccaagg ccaucaagaa gcugcucucc cugaugcgga ugggcaagua cuggagcgag     1920

gacgccaucg acgugaacac ccgcgagcgg aucgacaaga ucgucaccgg cgaguacgac     1980

gagaagaucc gcgaccgggu gcgcgagaag accauccggc ugaacgacau ccgccacuuc     2040

cgggcccucc ccgacuggcu ggcgugcuac gucguguacg accgccacuc cgagacgcgc     2100

gagaucaccg aguggaaguc ccccgaggag aucgacgagu acauccggaa cuucaagcag     2160

cacagccugc gcaaccccau cguggagcag gucaucaccg agacccuccg gacggugcgc     2220

gacaucugga agcaggucgg gugcaucgac gagauccacg uggagcuggg ccgggagaug     2280

aagaaccccg ccgacaagcg caagcggauc accagccaga ucaucgagaa cgagaacgcc     2340

aaccugcgcg ugaaggcccu ccugaccgag uucgugaacc ccgaguucga gaucgagaac     2400

guccggccgu acagccuguc ccagcaggac auccuccgca ucuacgagga cggggugcug     2460

aacagcgugg cggagguccc cggcgacauc gccgggaucc ugaagaaguu caacgagaac     2520

gacgccaaga agcggcccac cacguccgag auccuccgcu acaagcugug gcuggagcag     2580

aaguaccgga gccccuacac cggcgagaug aucccgcucu ccaagcuguu caccagcgcc     2640

uacgagaucg agcacgugau cccccagucc cgcuacuucg acgacagcuu cuccaacaag     2700

gugaucugcg aggccgaggu caacaagcug aaggacaacg cgcucggcua cgaguucauc     2760

aagaagcacc acggggagaa gguggagcug ggcuucggga agaucgugga gaucuucagc     2820

gucuccgugu acgagcaguu cgugaaggac aacuacagcc ggaaccgcgg caagaugaag     2880

aagcugcuca uggacgagau ccccgagcag uucaucgagc ggcagcugaa cgacucccgc     2940

uacaucagca aggucgugaa gacccugcuc uccaacgugg uccgggagaa ggccgagcag     3000

gaggccguga gcaagaacgu gaucgucugc accgggggcg ugacggaccg ccugaagaag     3060

gacuggggca ucaacgacgu guggaacaag aucguccugc cccgguuccu ccgccugaac     3120

gagcugacca acuccaccaa cuucaccgcc aagaacgccg cggggaacga gaucccggcc     3180

augccccucg agcugcagaa gggcuucaac aagaagcgca ucgaccaccg gcaccacgcc     3240

auggacgcca ucgugaucgc cugcgcgaac cgcaacaucg ugaacuaccu gaacaacgag     3300

agcgccagcc ggaaggccaa gaucucccgc cacgaccucc aggccauccu gugcgacaag     3360

guccggacgg acgacaaggg gaacuaccag uggcgcguga agaagcccug ggacaccuuc     3420

acccaggacg uguacgucgc gcuggagaac aucaucguga gcuucaagca gaaccuccgg     3480

gugaucaaca agaccuccaa cuacuacugg cacuacgaga acggcaagaa gguccuggug     3540

aagcagguca agggcgacag cugggccauc cgcaagccga ugcacaagga cacgguguuc     3600

ggggagguga accugcggaa ggugaagucc gucagccuca acgaggcccu gaagaacccc     3660

caguucaucg uggagaagga ccugaagcgc cagcuccugu cccugcucca gcagaacuuc     3720

gacaucaaga agaucaagaa guacuucgag gagcacaagg acaucuggag cgacaucucc     3780

cugagcaaga ugaacaucua cuacuucacc aaggagacca aggaccggua cuucgccacc     3840

cgcaagcccc uggacgccuc cuucgacaag aagaagaucg agagcugcau cacggacacc     3900

ggcauccagc agauccuccu gcggcaccug gagcucaagg agaacgaccc cgagcgcgcg     3960

uucuccccgg acgggaucga cgagaugaac cggaacauca uccagcugaa caagggcaag     4020

gggcaccacc ccaucgccaa ggugcgcguc uacgagaagg ccgacaaguu cgaggugggc     4080

cagcggggca acaagagcuc caaguucgug gaggccgcca aggggaccaa ccuguucuuc     4140

gcggucuacg agaccgagua ccccgacgag aagacgaaca gcaucgugcg caagcggaac     4200

uacgccacca ucccccucaa caucgccauc gagcgccaga agcgcggccu guccaccgcc     4260

ccggaggacg agaaggggaa cguccccagc uucgugcugu cccccaacga ccucguguac     4320

cugcccacca gcgaggacau cgucaacggc cacaucacgc agccgaucga caagagccgg     4380

aucuacaaga ugguguccag cuccgggagc cagugcuucu ucaucaagca caacaucgcc     4440

aacaucaucg uggacaagua cgaguucagc ccccugaaca agauggagcg cgcgaucagc     4500

aacgagauga ucaaggagau cugcaucccc gucaaggugg accggcucgg caacaucgug     4560

aagcugggca aguccgagug a                                               4581


<210>  2282
<211>  4584
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2282
auggccaaga uccugggccu cgaccugggg accaacagca ucggcugggc caucguggag       60

aaggacggga acgacuucuc ccuggucgac aagggcgugc gcaucuucag cgagggggug      120

aaguccgaga agggcaucga gagcucccgg gcggccgagc gcaccggcua ccggagcgcc      180

cgcaagauca aguaccggcg caagcuccgg aaguacgaga cgcugaaggu ccugucccuc      240

aacgggaugu gcccccugag caucgaggag guggaggagu ggaagaaguc cggcuucaag      300

gaguacccgc ugaaccccga guuccucgag uggcugcgca ccagcgacga cgagaacauc      360

aaccccuacu ucuuccggga caaggccucc aagcagaagg ugacccuguu cgaggucggg      420

cgcgccuucu accacaucgc gcagcggcgc ggcuuccuca gcaaccggcu ggaccagucc      480

gccgagggga uccuggagga gcacugcccc aagaucgagg cccucgugga ggaccugaac      540

agcaccgacg agauccugac gucccucaag gacuacuucu ucgacaccgg cauccuggac      600

gccaacgaga agggcggguu cgcgaaggac cuggacgagg gcgacaagaa gcucgugagc      660

cuguaccgcu ccauccaggc caucgucaag aagaacgaca ccgacuucga gaaggggaag      720

gccgagauca ucgagcggcu gaacaagaag gacgugcucg gcaaggugaa gggcaagauc      780

aaggacauca gccaggccau gcuggagggg aacuucaaga cccugggcca guacuucuuc      840

ucccucuaca acaaggagcg cauccggaac caguacacga gccgcgagga ccacuaccug      900

gaggaguuca agaucaucug caaguuccag gggaucggca gcaucaacca gaacgagaag      960

cugccggaga agaaguucga ggggcucgcc aaggagcugu acaaggcgau cuucuuccag     1020

cggccccuga agucccagaa gggccucauc ggcaagugca gcuucgagaa guccaagagc     1080

cgcugcgcca ucucccaccc cgacuucgag gagaagcgga ugugggagua ccugaacacc     1140

aucaagaucg ggacccagag cgagaagguc cugcgcuucc ucucccagga ggagaagcug     1200

aaccugaucc ccaaguuccu ccgcaagaag gacaacuuca acuucgagga ccuggccaag     1260

gagcugaucg agaagggcgc caccuucggg uucuacaaga gcuccaagcg ggacagcuuc     1320

cacuacuggu ucaacuacaa gccgacggac accguguccg gcugccccgu gagcgccucc     1380

cucaagagcg ucguggggga ggacuggaag accaaguccu ucaucuacaa gaccacgaac     1440

agcaagggca ucgagguguc ccgcaccguc aacuacaagg accuguggca ccuccucagc     1500

guguccacca gcgacaucua ccuguacgag uacgcgaaga acaagcuggg ccucgaggac     1560

aagaacgcca aggccuucag caagaucaag cugaagaagg acuucgggaa ccugucccug     1620

aacgccauca acaagauccu ccccuacaug cgggagggcc ugcuguacag ccacgccgug     1680

uucauggcga acaucgaguc caucgucgac gaggugaucu ggaacgaccu cgggcagcgc     1740

aaguacaucc aggacaagau cgccgccauc aucgccaaca acaucgugga caacggccug     1800

cuggaggucg ugaacgggcu caucaaggag ugcaaggucg acggcgccua cuacagcaag     1860

gaggcggccg acaccuaccg gaaggaccug uccaagaagc uggugagcuu cuucaaguuc     1920

caccacaucg acgacggcau caagcaggag acgauccuca acgagcuguu ccccguguuc     1980

aucgagcagc ugaaggccca cgaguucauc aaggucaagc gccucgacga gaaggugcug     2040

gaguuccuga agggggagaa ccaggacggc gagguguucu gcaacaacgc caagcagcuc     2100

aagaagcugu accacccguc cgacaucgag gucuucaaga agcagaucgu gaaggacaag     2160

gacgggaccg agaccggcca gcugauccuc ggcagccccc ugaccuccag caucaagaac     2220

cccauggcga ugcgggcccu gcaccagcuc cgcaaggugc ugaacacgcu gaucgucgag     2280

gagcaggucg acuccagcac ccggauccac aucgagaugg cccgcgagcu caacgacgcc     2340

aacaagcgga aggggaucca ggacuaccag aacgagaaca agaaggagcg cgagaaguac     2400

cgggacgaga ucaagaagcu guaccuggcc gagugcaaca aggagaucga gcccaccgug     2460

gacgaccucc ugcgcuacca gcuguggauc gagcaggacc ggaaggagau cuacgagaac     2520

ggcaacucca ucagcaucug cgacaucauc ggggacaacc cggcguacga caucgagcac     2580

accgugcccc gcuccaucag ccaggacaac ucccagauga acaagacgcu cugcagccag     2640

aaguucaacc gggacgugaa gaagcagaag augcccuucg agcugggcaa ccaccuggag     2700

auccuccccc gcaucgccca cuggaaggag gaggccgaca accugagccg ggagaucgag     2760

augaucaucc gcuccaccaa gaccgccgcc accaaggagg cgaaggacaa gaagauccgc     2820

cggcgccacu accugacgcu ccggcgcgac uaccugcagg ggaaguacga ccgguucauc     2880

uggaccgagc cgaagguggg cuucaagaac agccagaucc ccgacaccgg caucaucacc     2940

cgcuacgccc aggccuaccu gaaguccuac uucaaccggg ucgagagcgu gaagggcggg     3000

augguggccg aguuccgcaa gaucuggggg auccaggagu ccuaccagga gaacggcaag     3060

aaguacuuca aggagaagga ccggagcaag cacacgcacc acaccaucga cgccaucacc     3120

aucgcgugca ugaccaagga caaguacgac guccucgccc acgccuggac ccuggaggac     3180

aaggagcaga ucacggaggc ccgcaagauc guggcggagu ccaagcccug gaagaccuuc     3240

aaggaggacc ugaagaagau cgaggagggg auccucguga gccacuacac ccccgacaac     3300

gucaagaagc aguccaagaa gaucgugcgg gugcgcggcc ggaagcagua cgucgccgag     3360

aucgagcgcg acgugaacgg caaggccgug gccaagaagg acaucagcgg gaaggucauc     3420

uacaagcugg acaaggacgg caagaagcug ccgcggcucc agcaggggga caccauccgc     3480

ggcucccugc accaggacag caucuacggg gccaucaaga acccccugaa gacggaggag     3540

auccgguacg ugauccgcaa ggaccucgag uccaucaagg cgagcgacgu ggacaacauc     3600

gucgacgaga uggugaagga gaaggugaag gaggccaucg ccuccaaggu ccugauccug     3660

agcuccaacc cccagcagaa gaacaagauc gacggcaagg uguggaugaa cgaggagaag     3720

ggcaucccca ucaacaaggu gcggcucuac gccaacagcg ucaagaaccc gcuggagauc     3780

aaggagcacu ccagccugag caagucccgc cacgagcaca agcagaaggu guacgggcag     3840

aacgacgaga acuucgccau ggcgaucuac gagggccucg acaagaaggg gaagaccgag     3900

cgggccuucg agcugaucaa cuacauggac gccggcuacu acuacaagcu gaagaacaag     3960

aacgagcgca agaaggacau caucagccuc gugcccgaga agcacaucga gaccggguuc     4020

aaccugcugc gcuccaacaa gauccccauc auccucaaga agggccugaa cgucauccug     4080

uacaagaacg agaaggagga gaucgacuuc aacaacccga acagcaucau caagcggcuc     4140

uacaagaucu ccggccugga caaggacggg aucaagcuga uguacugcau cagcgccaag     4200

ggcuccaccg agaacaucgc cuacaugaac gacgugauca acaagcagaa gcuguucgag     4260

cucaagagcg agauggagaa gcuggggcug ugggacaagg uguucuccga cuucaagaac     4320

gacaaccuga agaaguccga cuucuucaag aagcugaacg accuccugaa caagcacuac     4380

uucgagaaca acgucugcga cagcaucggc agcaucaaga aggugaagau caaggagucc     4440

ggccugacga cccccaaggg cggggacgug aucgacaacu ucaaggacuu ccccuacauc     4500

aaguucaagg ucagcaacuu caacgcgcuc aucgagggga ucgacuucaa gaucuccccc     4560

accggcaagu ucgugcagau cuga                                            4584


<210>  2283
<211>  4587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2283
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccugguggag       60

caggacuuca agaacaagca ggggaacauc augggcaugg ggucccgcgu cauccccaug      120

agccaggaga uccucggcga guucgcccgg ggcaacacca aguccgagac ggcggagcgc      180

acccggcugc gcagcgugcg gcgccugcgg gagcgccacc uccugcggcg cgagcggcug      240

caccgcgugc uccacauccu gggguuccug ccgaagcacu acgucaacca gaucgacuuc      300

caggagcggc ucggcaaguu cuucgaggag uccgagccca agcuggccua cgacaccggg      360

gacuuccugu ucaagcccag cuuccucgag augcuggagg aguucaagau ccaccagccc      420

uccuucgugg agggcgacaa gaaggugccg uacgacugga ccaucuacuu ccugcgcaag      480

aaggcccuca cggagcggau cgagaaggag gagcuggccu gggucauccu gagcuucaac      540

cagaagcgcg gguacuacca gcuccggggc gaggacgagg aggagaagcc caacaagcug      600

gaggaguucu acucccugaa gaucguggac aucaaggugg acgagagccc caacaagaag      660

ggcgacaccu gguacucccu cacccuggag aacgggugga ucuaccgccg gagcuccaag      720

gucccccugg acgacuggcu cggcaagacc cgcgacuuca ucgugacgac cgacaucgac      780

gaggagggga acccgaagcu ggacaaggag ggcaacccca agcggagcuu ccgcgccccc      840

uccgaggacg acuggggccu gcgcaagaag aagaccgaga aggagauccu cgacagcucc      900

aagaccgugg ggcacuucau cuucgagaac cugcuggcgg accccacgca gaagaucaag      960

ggccggcucg uccgcaccau cgagcggaag uucuacaagg acgagcugaa gcagauccug     1020

gugagccaga accgcuucca cccggagcuc uccgacgccg acaugauggc cgacugcgug     1080

cgggagcugu acaagcacaa ccacagccac cagaacuucc uggccagcaa ggacaucaag     1140

caccuccugg ucgacgacau caucuucuac cagcgccccc ugcgguccca gaaguccacc     1200

aucgggaacu gcccccucga guucaagacc uucaagaagg acggcaucga gaucaaggug     1260

cccuacaagg uggcgcccaa gagccacccg cuguuccagg aguuccgccu gcuccagugg     1320

gucagcuccc ugcggaucau ccgcaagagc gacgacuccg acgugacgcg ggaguuccug     1380

gggacccagg acaagaccaa gcagcuccug gacuaccuga ugacccagag cgagauggac     1440

ugcaacaagc ugaugacguu ccuccugacc caggagggcu acaagcgcaa ggaccugaag     1500

uccgaggugg gcaaguuccg guggaacuac gucuucgaca gcgagaccgg ggaguccaag     1560

accuaccccg gcaacagcac gcgcgccgag uuccucaagc ggcuggggaa gguggagggc     1620

uucgacuucu ccaaguacac cgaggaguuc gaggagagcc uguggcaccu cguguacucc     1680

gucaccgaca agguggaguu cgagaaggcc cugcgcaagu ucagccggaa gaacgggauc     1740

gagaucaacu ccuucgucga gaaccugaag aaguucccgc ccuacaagag cgaguacgcc     1800

uccuacagcc ucaaggccau caagaagcug cuggcgcuca ugcgcuucgg cgagaacugg     1860

aaggaggaca gcaucgacuc caucagcaag caccggaucg agaagauccu gaccggcgag     1920

uacgaccccg agaucaagga caaggugcgc gagaucuccc gcgccuucga cgagguggag     1980

gacuuccagg ggcugcccgu cugguucgcc aacuacgugg uguacggccg gcacagcgag     2040

gccuccgagc ucaucaagug gacgagcucc gacgagaugg ccgacuucau caaggaguuc     2100

aagcagcaca gccugcgcaa ccccaucguc gagcaggucg ugcuggagac ccuccgggug     2160

gugaaggaca ucugggacaa guacgggcac ggccagaagg acuucuucga cgagauccac     2220

guggagcugg ggcgcgagcu gaagaacccg gcggagcagc gggcccgccu caccacccag     2280

aucuccgcca acgagaacac gaaccugcgg aucaagagcc ugcucgucga gcugcugcac     2340

gaccccggcg uggagaacgu gcgccccuac ucccccaugc agcaggagau ccucaagauc     2400

uacgaggagg gcguccucaa gagcuccuuc gaggugccgg acgacauccu gaagaucagc     2460

aagaccgccc agcccuccag cuccgaccug aagcgguaca agcuguggcu ggagcagaag     2520

uaccgcagcc ccuacaccgg gaagaucauc ccgcucaaca agcuguucac ccccgccuac     2580

gagguggagc acgucauccc ccagagccgg uacuucgacg acuccuucag caacaaggug     2640

aucugcgagg cggccgugaa caagcugaag gacaaccgcc ucggcaugga guucaucaag     2700

ugcuuccacg ggcagcgggu cucccugggc uucggcgagg agguggagau cuucagcgag     2760

uccgccuaca aggccuucau ccaggacaac uuccccaaga acccggccaa gaagcgcaag     2820

cugcucaugg acgagcugcc cgagcagaug guggagcggc agaugaacga cacgcgcuac     2880

aucagcaagu ucgucaccuc cauccugagc aacaucgugc gguccgagga gaacgacacc     2940

ggguucaaca gcaagaacau cauccccggc aacgggaaga ucaccaccgc gcucaagcag     3000

gacuggggcc ugaacgacgu guggaacgag cugauccucc cccgcuucga gcggaugaac     3060

cagcugacgg ggaccaccgu cuucaccgcc uggaacgaga accaccagaa guuccugccg     3120

acggugcccc ucgaguacgc caagggcuuc cagaagaagc gcaucgacca ccggcaccac     3180

gccauggacg cgcuggugau cgccugcgcc acccgcgacc acgucaacua ccugaacaac     3240

gagcacgccc ucggcaagaa cuacaagggg aaggaggaga agcagucccg ccgguucgac     3300

cugcgccaga agcugugccu caagaagaag aacggccaca gccaggagca guacgagugg     3360

aucuucucca agcccuggga cagcuucacc caggacgcca agaagaaccu ggagcggauc     3420

aucguguccu ucaagcagaa ccugcgcgug aucaacaaga ccagcaacaa guaccucucc     3480

uacaaggacg aggaggggaa ccugcggcug gacagccagg gcaagcccgu caaggcgcuc     3540

acgcgccaga ucaaggggga cgccugggcc auccggaagu ccauccacaa ggacaccgug     3600

agcggccugg ugaagcugcg ccgggucaag auggugaacc ucagcguggc ccuggacaac     3660

ccggagaaca uccugaacaa gaagcucaag aagaagaucg ccgagcuggu caagcugaac     3720

uacgacaaca agcuccugcu gaaguacuuc cgcgaccggg acuacauguu cgacggccgc     3780

gaggugucca agcucgacgu guacuacuuc gaggaggggc uggcggccag ccgggucucc     3840

cuggacacca gcuucuccga caagaagauc cagagcauca ccgacacggg cauccgcaag     3900

auccuccugg cccaccuggc caacuaccac gggcggcucg acgagaaggg caaggugauc     3960

ccgcccgaga cccuggccuu cacccccgag gggaucgagg agcugaacaa gaacaucaag     4020

ggccucaacg gcgggaaguu ccaccagccc aucuucaagg ugcgcaccua cgagcccaag     4080

ggcaacaagu uccaggucgg guucaagggc aacaagaacu ccaaguacgu ggaggcggcc     4140

aaggggacga accuguucuu cgccaucuac cugaacgacg agggcaagcg gagcuacgag     4200

accauccccu ucaacguggu cauggagcgc cucaagcagg gcgaggggcc cgugccgcag     4260

accaacgaga agggcgggaa gcugcuguuc caccucuccc ccaacgagcu gguguacguc     4320

cccgagaccg acgagaacua cacgaacgug gacuucaaga accugagcaa ggagcagguc     4380

gagaagaucu acaagauggu guccagcacc ggcuccgagu gccacuucgu gcgggccgac     4440

aucagcuccc ucaucaaggg cuacgacgcg aagaccaagg ucggggagcu gggcagccug     4500

aacaagcagg aggugaccaa caacgggaug acgcgcauca aggagaagug cuggaagauc     4560

aagaucgacc gccucggcuc caucuga                                         4587


<210>  2284
<211>  4587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2284
augaagaaga uccugggcau cgaccucggg accaacagca ucggcugggc cguggucaac       60

uccuucgacg agaaccagaa cgaggagagc cugaagcaca ucgagugcgu ggggucccgc      120

aucaucccca uggacgccgc gauccugggc gacuucgaca aggggaacag caucucccag      180

accgccaagc ggacggaguu ccgcagcgug cggcaccucc gcgagcggca ccuccugcgc      240

cgggcccgcc uccacaagau ccugucccug cucggcuucc ugccggacca cuacguccag      300

gaccuggaca gccacggcaa cuuccccgug aacguggagc ccaagaucgc cuggcggaag      360

gacuccaacg gggccuucga guucaucuuc aacgacagcu ucgacgagau gcuccaggac      420

uucgcgaccu accagcccga gcugaucgcc ugcaacaaga aggucccgua cgacuggacc      480

aucuacuacc ugcgcaagaa ggcccucacc aagaagcugu ccaaggagga gcugagcugg      540

auccuccuga acuucaacca gaagcggggc uacuaccagc ugcgcgggga ggaggacgac      600

gaguccguca agagcgccaa gacgcggcag uacuucgacc gccagaagau caaguccauc      660

guggacacca accagaccua caagggccag aaggugcuca ucguggagcu ggaggacggg      720

aucaagggca agaucuucaa gaaggagauc cccaacuggg ugggccagga gaaguccauc      780

aucgucacca ucgacaucga caaggacggg ugcgacaagu acgaggagag cggcgagcug      840

agcugccggu ucaagauccc cuccgacgcg gagugggaga gcgaguggaa gcucaucaag      900

augaagacgc agaaggaccu ggacgccagc gggaagaccg ugggcgccua caucuacgac      960

gccauccugc agaaccccuc ccagaagauc cgcggcaagc ucgugcggac caucgagcgc     1020

aaguacuaca aggaggagcu gaagcagauc cugaagaagc aggucgaguu ccacccggag     1080

cuccgggaca aggaccuguu ccuggacugc cucaacgagc uguaccccaa caacgacgcc     1140

caccgcaacg cgugcgccga ccgggacuuc aucuaccugu ucauggacga cauccucuuc     1200

uaccagcgcc cccucaagag caagaagucc cugaucgccg acugccccua cgagaagcac     1260

auguaccgcg acaaggagac cggggagcug aaguacagcu ccgugaagug caucgccaag     1320

agccacccgc uguuccagga guuccggcug uggcacuuca ucuccaacau ccgcaucuac     1380

cagaaggagc ggguggucga cguggacgcc gaccagagcg cgcucuccag cgcccgcucc     1440

gcccucaaga gcaagcugga ggccgacgug gacgucacga acgaguuccu gaccugcgag     1500

gccgacuacg ugucccuguu cgaguggcug aacaacaaga aguccgugag ccagaaggac     1560

uuccugaagu accccgcguu cggcaucaag aaguccgggg acaacuaccg guggaacuac     1620

gucgaggaca agagcuaccc cugcaacgag acccgcgccc ugauccugag ccaccuggag     1680

aaggccaaca uccggggcga caucuucacc cgcgagcagg aggaggcccu cuggcacauc     1740

cuguacuccg uggaggaccg guacgagauc gucaaggccc ugcagagcuu cgcggccaag     1800

uaccgcuuca acgacgacuu cgugacggug uucagcaagu ucccgcccuu caagaaggag     1860

uacggguccu acagcgccaa ggccaucaag aagcuccugc cccugaugcg gaugggcaag     1920

uacugguccg aggcggccau cgacaucaag acccgcgagc ggaucgagaa gaucaucacc     1980

ggcgagugcg acgacagcau ccgcgagcgg guccgccuca aggccauccg gcugcacgac     2040

aucggggacu uccgcgcccu gcccgugugg cucgccugcu acguggucua cgaccggcac     2100

uccgagagcg gcgacaucac caaguggaag acgccccagg agauggacug guacauccag     2160

aacuucaagc agcacucccu gcgcaacccg aucguggagc aggucaucac cgagacccug     2220

cgcguggugc gggacaucug gaagcagguc gggcgcaucg acgagaucca cguggagcuc     2280

ggccgggaga ugaagaaccc cgcggacaag cgcgccaaga ugaccgcccg gaugaucgag     2340

aacgagaaca cgaaccugcg caucaaggcc augcugaccg aguuccucaa ccccgaguuc     2400

gagaucgaga acgugcggcc cuacagcgcc ucccagcagg acauccugcg caucuacgag     2460

aacggggugc ugaacagcgu cuccgagauc ccggaggaga ucaccggcau ccucaagaag     2520

uucaacgagc gggagcagcg caagcggccc agcaccuccg aggugcugcg cuacaagcug     2580

uggcucgagc agaaguaccg gagccccuac acgggcaaga ugaucccccu ggggaagcug     2640

uucaccccgg cguacgagau cgagcacauc aucccccagu cccgcuacuu cgacgacagc     2700

uucaacaaca aggugaucug cgaggccgag gucaacaagc ucaagggcaa ccugcugggg     2760

uacgaguuca ucaagaagca ccacggccgg cgcguggacc ucgggcuggg cgagaucgug     2820

gacaucuucu ccgucgacga guacgagacc uucgugaagg acuacuacag cgccaaccag     2880

cagaagauga agaagcugcu cauggacgag auccccgagc aguucaucga gcggcagcug     2940

aacgacagcc gcuacaucuc caaggugguc aagagccugc ucuccaacau cgugcgggag     3000

caggacgagc aggagagcau cuccaagaac cugaucgugu gcaccggcgg ggucacggac     3060

cgccugaaga aggacugggg caucaacgac guguggaaca agcucauccu gccgcgguuc     3120

cagcgccuga acaacaucac cgagaccacc gaguucacgg ccaagaacau gcaggggcac     3180

gagauccccg ugguccccuu cgaguaccag cgcggcuuca acaagaagcg gaucgaccac     3240

cgccaccacg ccauggacgc gaucgugauc gccugcgccg gccggaacau cgugaacuac     3300

cucaacaacg agagcgcccg caagaaggcg cagaucuccc gguacgaccu gcagcgccug     3360

cucugcgaca aggucaagac cgacgacaac gggaacuaca aguggauccu gaagaagccc     3420

uggagcaccc ugacccagga cguguacgcc gcccucgaca acgugaucgu cuccuucaag     3480

cagaaccugc gggugaucaa caagaccaac aaccacuacg ugcacuacga ggccggcaag     3540

aagaucaucg ccaagcagga gaaggcgagc ugggccaucc gcaagccgau gcacaaggac     3600

acggucuucg gggaggugaa ccugcggaag gugaagaccg ucucccucaa cgaggccauc     3660

aagagccccc acugcaucgu gcagaaggac uucaagaaga agcugcuguc ccuccugaag     3720

cagaacuucg acgccaagga ggugaaggcc uacuucgagg agcacaagga cgucuggagc     3780

gacaucaacc uguccaagau cgagguguac uacuucacca aggacaccaa ggagcgcuuc     3840

uucgcgacgc ggaagccccu cgacagcucc uucgaccgca agaagaucga gagcgugacc     3900

gacaccggca uccagaagau ccugcugcgg caccucgagc agaagaacgg ggacuccgac     3960

cuggccuuca gccccgacgg caucgaggag augaaccaga acaucgucca gcugaacggc     4020

gggcgcaagc accagccgau cgugaaggug cgggucuacg agaaggccga caaguucgcc     4080

aucggccagc aggggaacaa ggccagcaag uacguggagg cggccaaggg caccaaccuc     4140

uucuucgccg ucuacgagga ggaggugcgc gacaagaaga cgaacgccgu gauguucaag     4200

cggaacuacg cguccauccc ccugaacguc gccaucgacc gccagaagaa ggggcugagc     4260

accgcccccg aggacgagaa cggcaacgcc cccaucuucg ugcucucccc gaacgaccug     4320

guguaccugc ccaccgccga ggacaucauc aacgcgcgga ucagcuuccc caucaacaag     4380

aaccgcaucu acaagauggu cuccugcacc ggcuaccagu gcuucuucau caagcaccac     4440

guggccaacg ugaucgucga caagaaggag uucagcgccc ucaacaagau ggagaaggcc     4500

gagacgaacg agaugaucaa ggacaucugc gugcccauca agcuggaccg ccuggggaac     4560

aucgugaaga ucggcgucua caaguga                                         4587


<210>  2285
<211>  4587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2285
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cguggucaac       60

uccuucgacg agaaccagaa cgaggagagc cugaagcaca ucgagugcgu ggggucccgc      120

aucaucccca uggacgccgc gauccucggc gacuucgaca aggggaacag caucucccag      180

accgccaagc ggacggaguu ccgcagcgug cggcaccucc gcgagcggca ccuccugcgc      240

cgggcccgcc ugcacaagau ccugucccuc cugggcuucc ugccggacca cuacguccag      300

gaccucgaca gccacggcaa cuuccccgug aacguggagc ccaagaucgc cuggcggaag      360

gacuccaacg gggccuucga guucaucuuc aacgacagcu ucgacgagau gcugcaggac      420

uucgcgaccu accagcccga gcugaucgcc ugcaacaaga aggucccgua cgacuggacc      480

aucuacuacc uccgcaagaa ggcccugacc aagaagcugu ccaaggagga gcucagcugg      540

auccugcuga acuucaacca gaagcggggc uacuaccagc uccgcgggga ggaggacgac      600

gaguccguca agagcgccaa gacgcggcag uacuucgacc gccagaagau caaguccauc      660

guggacacca accagaccua caagggccag aaggugcuga ucguggagcu ggaggacggg      720

aucaagggca agaucuucaa gaaggagauc cccaacuggg ugggccagga gaaguccauc      780

aucgucacca ucgacaucga caaggacggg ugcgacaagu acgaggaguc cggcgagcuc      840

agcugccggu ucaagauccc cuccgacgcg gagugggaga gcgaguggaa gcugaucaag      900

augaagacgc agaaggaccu ggacgccagc gggaagaccg ugggcgccua caucuacgac      960

gccauccucc agaaccccuc ccagaagauc cgcggcaagc uggugcggac caucgagcgc     1020

aaguacuaca aggaggagcu gaagcagauc cucaagaagc aggucgaguu ccacccggag     1080

cugcgggaca aggaccuguu ccucgacugc cugaacgagc uguaccccaa caacgacgcc     1140

caccgcaacg cgugcgccga ccgggacuuc aucuaccucu ucauggacga cauccuguuc     1200

uaccagcgcc cccucaagag caagaagucc cugaucgccg acugccccua cgagaagcac     1260

auguaccgcg acaaggagac cggggagcug aaguacagcu ccgugaagug caucgccaag     1320

agccacccgc uguuccagga guuccggcuc uggcacuuca ucuccaacau ccgcaucuac     1380

cagaaggagc ggguggucga cguggacgcc ugccagcccg accugagcuc cgcgcgcagc     1440

gcccugaagu ccaagcucga ggccgacgug gacgucacga acgaguuccu gaccugcgag     1500

gccgacuacg ugagccuguu cgaguggcuc aacaacaaga aguccgugag ccagaaggac     1560

uuccugaagu accccgccuu cggcaucaag aaguccgggg acaacuaccg guggaacuac     1620

gucgaggaca agagcuaccc cugcaacgag acccgcgcgc ugauccuguc ccaccucgag     1680

aaggccaaca uccggggcga caucuucacc cgcgagcagg aggaggcccu guggcacauc     1740

cuguacagcg uggaggaccg guacgagauc gucaaggccc uccaguccuu cgccgcgaag     1800

uaccgcuuca acgacgacuu cgugacggug uucagcaagu ucccgcccuu caagaaggag     1860

uacgggagcu acuccgccaa ggccaucaag aagcugcugc cccucaugcg gaugggcaag     1920

uacuggagcg aggccgcgau cgacaucaag acccgcgagc ggaucgagaa gaucaucacc     1980

ggcgagugcg acgacuccau ccgcgagcgg guccgccuga aggccauccg gcugcacgac     2040

aucggggacu uccgcgcccu ccccgugugg cuggccugcu acguggucua cgaccggcac     2100

agcgaguccg gcgacaucac caaguggaag acgccgcagg agauggacug guacauccag     2160

aacuucaagc agcacagccu gcgcaacccc aucguggagc aggucaucac cgagacccuc     2220

cgcguggugc gggacaucug gaagcagguc gggcgcaucg acgagaucca cguggagcug     2280

ggccgggaga ugaagaaccc cgccgacaag cgcgcgaaga ugaccgcccg gaugaucgag     2340

aacgagaaca cgaaccugcg caucaaggcc augcucaccg aguuccugaa ccccgaguuc     2400

aagaucgaga acgugcggcc guacuccgcc agccagcagg acauccugcg caucuacgag     2460

aacggggugc ucaacuccgu cagcgagauc cccgaggaga ucaccggcau ccugaagaag     2520

uucaacgagc gggagcagcg caagcggccc uccaccagcg aggugcugcg cuacaagcuc     2580

uggcuggagc agaaguaccg gucccccuac acgggcaaga ugaucccgcu ggggaagcug     2640

uucacccccg ccuacgagau cgagcacauc aucccccaga gccgcuacuu cgacgacucc     2700

uucaacaaca aggugaucug cgaggcggag gucaacaagc ugaagggcaa ccugcucggg     2760

uacgaguuca ucaagaagca ccacggccgg cgcguggacc uggggcuggg cgagaucgug     2820

gacaucuuca gcgucgacga guacgagacc uucgugaagg acuacuacuc cgccaaccag     2880

cagaagauga agaagcuccu gauggacgag aucccggagc aguucaucga gcggcagcug     2940

aacgacagcc gcuacaucag caaggugguc aagucccucc ugagcaacau cgugcgggag     3000

caggacgagc aggaguccau cagcaagaac cugaucgugu gcaccggcgg ggucacggac     3060

cgccucaaga aggacugggg caucaacgac guguggaaca agcugauccu gccccgguuc     3120

cagcgccuca acaacaucac cgagaccacc gaguucacgg ccaagaacau gcaggggcac     3180

gagauccccg ugguccccuu cgaguaccag cgcggcuuca acaagaagcg gaucgaccac     3240

cgccaccacg ccauggacgc caucgugauc gcgugcgccg gccggaacau cgugaacuac     3300

cugaacaacg aguccgcccg caagaaggcc cagaucagcc gguacgaccu gcagcgccuc     3360

cugugcgaca aggucaagac cgacgacaac gggaacuaca aguggauccu gaagaagccg     3420

ugguccaccc ucacccagga cguguacgcg gcccuggaca acgugaucgu cagcuucaag     3480

cagaaccugc gggugaucaa caagaccaac aaccacuacg ugcacuacga ggccggcaag     3540

aagaucaucg ccaagcagga gaaggccucc ugggcgaucc gcaagcccau gcacaaggac     3600

acggucuucg gggaggugaa ccuccggaag gugaagaccg ucagccugaa cgaggccauc     3660

aagagccccc acugcaucgu gcagaaggac uucaagaaga agcugcucag ccugcugaag     3720

cagaacuucg acgccaagga ggugaaggcc uacuucgagg agcacaagga cgucuggucc     3780

gacaucaacc ucagcaagau cgagguguac uacuucacca aggacaccaa ggagcgcuuc     3840

uucgccacgc ggaagccccu ggacuccagc uucgaccgca agaagaucga guccgugacc     3900

gacaccggca uccagaagau ccugcuccgg caccuggagc agaagaacgg ggacagcgac     3960

cuggcguucu ccccggacgg caucgaggag augaaccaga acaucgucca gcucaacggc     4020

gggcgcaagc accagcccau cgugaaggug cgggucuacg agaaggccga caaguucgcc     4080

aucggccagc aggggaacaa ggccagcaag uacguggagg ccgcgaaggg caccaaccug     4140

uucuucgccg ucuacgagga ggaggugcgc gacaagaaga cgaacgccgu gauguucaag     4200

cggaacuacg ccagcauccc ccugaacguc gcgaucgacc gccagaagaa ggggcucucc     4260

accgcccccg aggacgagaa cggcaacgcc ccgaucuucg ugcugagccc caacgaccug     4320

guguaccucc ccaccgccga ggacaucauc aacgcccgga ucuccuuccc caucaacaag     4380

aaccgcaucu acaagauggu cagcugcacc ggcuaccagu gcuucuucau caagcaccac     4440

guggcgaacg ugaucgucga caagaaggag uucuccgccc ugaacaagau ggagaaggcc     4500

gagacgaacg agaugaucaa ggacaucugc gugccgauca agcuggaccg ccucgggaac     4560

aucgugaaga ucggcgucua caaguga                                         4587


<210>  2286
<211>  4590
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2286
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc cguggucaac       60

uccuucgacg agaaccagaa cgaggagagc cugaagcaca ucgagugccu cgggucccgc      120

aucaucccca uggacgccgc gauccugggc gacuucgaca aggggaacag caucucccag      180

accgccaagc ggacggaguu ccgcggcgug cggcaccucc gcgagcggca ccuccugcgc      240

cgggcccgcc ugcacaagau ccuccggcug cugggcuucc ucccggacca cuacgugcag      300

gaccuggacg ggcacggcaa cuuccugguc aacguggagc ccaagaucgc cuggcgcaag      360

gacagcaacg ggaccuucga guucaucuuc aacgacuccu ucgacgagau gcuccaggac      420

uucgccaccu accagcccga gcugaucgcg ugcaaccgga aggugcccua cgacuggacc      480

aucuacuacc ugcgcaagaa ggcccucacg aagaagcuga gcaaggagga gcuguccugg      540

auccuccuga acuucaacca gaagcggggc uacuaccagc ugcgcgggga ggaggacgac      600

gagagcguca agaccgccaa gacccggcag uacuucgacc gccagaagau caaguccauc      660

guggacacca accagacgua caagggccag aaggugcuca ucgucgagcu ggaggacggc      720

gugaagggga agaucuucaa gaaggagauc ccgaacuggg ugggccagga gaaguccauc      780

aucgucaccg uggacaucga caaggacggg ugcgacaagu acgaggagag cggcgagcug      840

agcugccggu ucaagauccc cuccgacgcc gagugggaga gcgaguggaa gcucaucaag      900

augaagaccc agaaggaccu ggacgcgucc ggcaagaccg ugggggccua caucuacgac      960

gccauccugc agaaccccag ccagaagauc cgcggcaagc ucguccggac gaucgagcgc     1020

aaguacuaca aggaggagcu gaagcagauc cugaagaagc aggucgaguu ccaccccgag     1080

cuccgggaca aggaccuguu ccuggacugc cucaacgagc uguacccgaa caacgacgcc     1140

caccgcaacg ccugcgcgga ccgcgacuuc aucuaccugu ucauggacga cauccucuuc     1200

uaccagcggc cccugaaguc caagaagucc cugaucgccg acugccccua cgagaagcac     1260

auguaccgcg acaaggagac cggggagcuc aaguacagca gcgugaagug caucgccaag     1320

agccaccccc uguuccagga guuccggcug uggcacuuca ucuccaacau ccgcaucuac     1380

cagaaggagc gggugaucga cguggacgcc ugccagccgg accucagcuc cgcccgcagc     1440

gcgcugaagu ccaagcugga ggccgacgug gacgucacca acgaguuccu gaccugcgag     1500

gccgacuacg ugagccuguu cgaguggcug aacaacaaga aguccgugag ccagaaggac     1560

uuccugaagu accccgccuu cggcaucaag aaguccgggg acaacuaccg guggaacuac     1620

gucgaggaca agagcuaccc cugcaacgag acgcgcgccc ucauccuguc ccaccuggag     1680

aaggcgaaca uccggggcga caucuucacc cgcgagcagg aggaggcccu cuggcacauc     1740

cuguacagcg uggaggaccg guacgagauc gucaaggccc ugcaguccuu cgccgccaag     1800

uaccgcuuca acgacgacuu cgugaccgug uucagcaagu ucccgcccuu caagaaggag     1860

uacggcuccu acagcgcgaa ggccaucaag aagcuccugc cccugaugcg gauggggaag     1920

uacugguccg agagcgccau cgacaucaag acccgcgagc ggaucgagaa gaucaucacg     1980

ggcgagugcg acgacagcau ccgcgagcgg guccgccuca aggccauccg gcugcacgac     2040

aucgagcacu uccgcgcgcu gcccgugugg cucgccugcu acguggucua cgaccgccac     2100

uccgagagcg gggacaucau gaaguggaag acgccccagg agauggacuc cuacauccag     2160

aacuucaagc agcacagccu gcggaacccg aucguggagc aggucaucac cgagacccug     2220

cgcguggugc gggacaucug gaagcagguc ggccgcaucg acgagaucca cguggagcuc     2280

gggcgggaga ugaagaaccc cgccgacaag cgcaaggaga ugaccaagcg gaucaucgag     2340

aacgagaaca ccaaccugcg caucaaggcc augcugaccg aguuccucaa ccccgaguuc     2400

gagaucgaga acgugcggcc cuacuccgcc agccagcagg acauccugcg caucuacgag     2460

aacggcgugc ugaacuccgu cagcgagauc ccggaggaga ucaccggcau ccucaagaag     2520

uucaacgagc gggagcagcg caagcggccc uccacgagcg aggugcugcg cuacaagcug     2580

uggcucgagc agaaguacug cucccccuac accgggaaga ugaucccccu gggcaagcug     2640

uucaccccgg cguacgagau cgagcacgug aucccccaga gccgguacuu cgacgacucc     2700

uucaacaaca aggucaucug cgaggccgag gugaacaagc ucaaggggaa ccugcugggc     2760

uacgaguuca ucaagaagca ccacgggcag cgcguggacc ucggccuggg cgagaucguc     2820

gacaucuuca gcguggacga guacgagacc uucgugaagg acuacuacuc cgggaaccag     2880

cagaagauga agaagcugcu cauggacgag auccccgagc aguucaucga gcggcagcug     2940

aacgacagcc gcuacaucuc caaggucguc aagagccugc ucagcaacau cgugcgggag     3000

caggacgagc aggaguccau cagcaagaac gugaucgugu gcacgggcgg ggugaccgac     3060

cgccugaaga aggacugggg caucaacgac gucuggaaca agcugauccu cccgcgguuc     3120

cagcgccuga acaacaucac cgagaccacg gaguucaccg ccaagaacau ggagggccac     3180

gagauccccg uggugcccuu cgaguaccag cgcggguuca acaagaagcg gaucgaccac     3240

cgccaccacg ccauggacgc caucgucauc gcgugcgccu cccggaacau cgugaacuac     3300

cugaacaacg agagcgcccg caagaaggcc cggaucuccc gcuacgaccu ccagaagcug     3360

cugugcgaca aggugcggac cgacgacaac ggcaacuaca aguggauccu caagaagccc     3420

ugggggaccc ugacccagga cgucuacgcc gcgcuggaca acgugaucgu gagcuucaag     3480

cagaaccucc gcgucaucaa caagacgaag aacuacuacg ugcacuacga ggccggcaag     3540

aagaccaucg ccaagcagga gaaggggucc agcugggcca uccggaagcc gaugcacaag     3600

gacaccgugu ucggcgaggu caaccugcgc aaggugaaga ccgugucccu gaacgaggcg     3660

aucaagaacc cccagugcau cgucgagaag gacuucaaga agaagcuccu gagccugcuc     3720

aagcagaacu ucgacgccaa gaaggugaag gccuacuucg aggagcacaa ggacgugugg     3780

uccgacauca accugagcaa gaucgagguc uacuacuuca cgaaggacac caaggagcgg     3840

uucuucgcca cccgcaagcc ccuggacucc agcuucgaca agaaggagau cgaguccgug     3900

accgacacgg gcauccagaa gauccuccug cggcaccugg agcagaagaa cggggacccc     3960

gaccucgccu ucagcccgga cggcaucgag gagaugaacc acaacaucgu gcagcugaac     4020

gggggccgca agcaccagcc caucgucaag gugcgggucu acgagaaggc ggacaaguuc     4080

gccaucgggc agcagggcaa caaguccagc aaguacgugg aggccgccaa gggcaccaac     4140

cuguucuucg ccguguacga ggaggagguc cgcgacaaga agaccaacgc ggugaugcuc     4200

aagcggcacu acgccuccau cccccugaac guggccaucg accgccagaa gaaggggcug     4260

agcaccgccc ccgaggacga gaacggcaac gucccgaucu ucgugcucag ccccaacgac     4320

cugguguacc ugcccacggc cgaggacauc gcgaacgccu ccaucagcuu ccccaucaac     4380

aagaaccgga ucuacaagau ggucuccugc accgggaacc gcugcuucuu caucaagcac     4440

cacguggcca acgugaucgu cgacaagagc gaguucuccg ugcucaacaa gaucgagaag     4500

gccgagaccg acgagaugau caaggagauc ugcgugccga ucaagaucga ccgccugggc     4560

aacaucguca agaucggggu guacaaguga                                      4590


<210>  2287
<211>  4608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2287
augaacacca uccugggccu cgaccugggg accaacagca ucggcugggc ccugaucaag       60

gugcucaacg acaagacgcg ccugccccug gagaucaucg accucggggu cguggugauc      120

ccgaccgacc agcggacccu ggacaaguuc uccaagggcg agagcaucuc caagaccgcc      180

gagcgcacga agcagaaggg gguccggaag cuguacgagc gcaagaagcu ccggcgcgag      240

cggcugcacc gcgugcugaa cauccucggc uuccugcccg agcacuacag caagaagauc      300

gacuucaaga agcaccuggg ccaguucuuc gggaacuccc agcccaagau cagcuacgug      360

gagaacgacc acggcaagca cgaguucauc uucauggacu ccuaccggga gaugcucaag      420

gaguucagca agcacaacau caagaacguc cccuacgacu ggaccaucua cuaccugcgc      480

aagaaggcgc ugucccagaa gaucagcaag gaggagcucg ccugggugau ccugaacuuc      540

aacaagaagc ggggguacua ccaguuccgc gacgacaacg ccgacgagaa caaggagaac      600

aagaucgaga aguacuacgc ccugaaggug accaacguca agaaggagga cgcccagaac      660

uacaccauca cgcucgagaa cggcuggauc uacaagaaca aguccaagau cagccugcug      720

gacugggugg gguccaccaa ggaguucauc agcaccgaga aguucgacaa caagggcaac      780

aucaucaaga agaagaacgg cgaggacgac cggacccuca agcuggugga cuccgagaag      840

gacuggaucg cgaucaagaa guccacggag gaccgcaucg agaacuacgg gaagaccguc      900

ggcaccuacc uguacgagca ccuccugaag gacccgaacc agaagcugaa cggggagcuc      960

aucaacauca ucgaccggaa guucuacaag aacgagcuga agcagauccu ggagaagcag     1020

aaggaguucc accccgagcu ccugaacgag gaccuguucc agaaguccau caucgagcuc     1080

uaccccaaga acagcacccg ccagaaggag cugaagaaca aggaccugug cuaccuccug     1140

aucgacgacg ugaucuacua ccagcgggac cugaagaaga agaacagcac gaucaacaag     1200

ugcagcuucg agagccgcga guacuaccuc aacggcgaga agcagaucca gaagaucaac     1260

ugcaucgcca aguccaagcc ccuguaccag gaguuccgga cccugcaguu caucaagaac     1320

cucaaggugu acgagaucau cgacggccug gagaaggaca ucaccagcaa gcugcucccg     1380

gacuucgaga ccaaggagca cuucuucgag uggcugaacg acaaggagaa gaucgaccag     1440

cugcagcugc ucuccuacaa gugcuucgac cugcacgaca acgucaagaa cgaccugauc     1500

gccaagggga ucaagcgccc cacggagaag gccauccgga ccgagcuccu ggagaaguac     1560

cgcuggaacu acagcaagga caacgaccgc aacuuccugc ucaacgagac ccgguucaag     1620

auccugaagu accugaagaa gguggacaag aagcucaccc ugacgaacga gcagaucgag     1680

ucccuguggc uccugcugua cagcgcgaag gacgagcgcg agcucaagaa cggccugcag     1740

aaguucuccg agaagaacaa gcugcccaag aaguucagcg acaucuucau cuccuucccg     1800

cccuucaaga aggaguacgg ggccuacagc gagaaggccc ucaagaagcu gcugucccuc     1860

gugcgguucg gcaaguacug gagcuucaac aagaucgaca acaacaucca gaacaagauc     1920

augaacaucc ugaacgggga ggucgacgac uccaucagcg accgcgugcg ggagaagacc     1980

auccugcucc aggacaagaa cgacuuccag gaccugcccc uguggcucgc cuccuacgug     2040

gccuacaacc gcuucagcga ggucuccgag aucaaguacu gggacagccc cgacaagauc     2100

gaguuccuga agcagcacuc caucggcaac cccaucgugg agaagaucau caacgagacc     2160

cugaagcuca ucaaggacau cugggugauc cacggcaaga gcugcaagaa cuucuucagc     2220

gagauccaca ucgagcuguc ccgggagauc aagagcucca gcaagaagcg cgcgcagauc     2280

accaacaaca acacgaagaa ccagaagcug aacaaccuca ucaagucccu gcugaucgag     2340

auggccagcg acaccaagua cgagaacguc accccguacu cccccagcca gcaggagauc     2400

cucaagaucu acgacgaggg gaucuacucc aaggaggaga acagcgagaa gaucaaggag     2460

aucaacaaga uccggaacaa caacaagauc uccaagagcg acaucaccaa guacaagcug     2520

uggcuggagc agggcuacau cucccccuac acggggaaga ucaucccccu caccaagcug     2580

uucaccgcca aguaccagaa ggagcacauc aucccgcaga ccaaguucuu cgacgacagc     2640

uucaacaaca aggugaucug cgaggccgag gucaacaagc ugaaggacaa cucccucgcc     2700

augaacuuca ucaaggcgaa gggcgggaag gccgugaagc ugagcgacgg cgagacggug     2760

accauccuga accaggagga guacaagcag cucguccgca ccuccuucaa gaagaacagc     2820

aucaagcugc ggaaccugcu ccuggaggag auccccgaca ccuucaccaa gcgccagcug     2880

aacgacacga aguacaucag caagcucgug cugucccugc ucagcaacau cgugcgggag     2940

aagggcgagc uggaguccac cgccaagcgc aucgucagca ccaacgggca gaucaccucc     3000

auccugcgga acgacugggg ccucaacaac aucuggaaca agcugaucac gccccgcuuc     3060

gagcggcuga acaacaucaa caagaacaag aucuacggga acugggagaa caagaagggc     3120

aagaacgugu uccagaucaa cuaccucaac gacaaccuga gcacccugaa caccaagcgc     3180

aucgaccacc ggcaccacgc ccucgacgcc cugaucgugg cgugcaccac gcgcaaccac     3240

aucaacuucc ugaacaacca guacgccaag ucccgcaacc agcggcacga ccuccgcgcc     3300

aagcugaagc ggcgcgagac caucaccacc gaggggagca agaagcaggu cuucaaggac     3360

uucaucaagc ccugggaguc cuucacgcag gacgcccuga agaagcucga gaccaccauc     3420

gugaacuuca aggugaacaa gcggaucauc aacaagacca ucaacuggua caagaagugg     3480

gagaagcaga aggacggcac guacaagaag auccagaaga agcagaccaa gggcgacgcc     3540

ugggcgaucc gcaagccgcu gcacaucgac accaucuacg ggaucgucug caagaagcug     3600

ccccucaaga gcgcccugga gaacuacaag aacaucaucg acgagaaccu gaagcacaag     3660

aucaaggagc ucaaggaggu gaacaaguuc gacaacaacc agaucaucaa cuucuucaag     3720

gagugcaacu ucaucuucga ggacaacaac auccagaagg uggaggucua cgacuucaag     3780

gacaaccggg acaagaccau ccuguccagc ucccgccagc ugaucgacga gagcuucacg     3840

cccaagaaga ucggcaaggu gcucgacccc accauccgga acauccugcu gaagcaccuc     3900

aacuccacca aguacaacaa caaguucgac agcgacggga acccgaucaa gcccgagaag     3960

cuggccuucu cccccgaggg ccugaaggac cucaacaaga acaucaagca ccugaacaac     4020

aacaaggagc accagccgau caagcgcgug aagcuguucg agacccucgg caacaagauc     4080

aaggucgggu acacgcagca gaagaacaag aaguucgccg agaccgcgac cggcaccaac     4140

cuguuccugg ggaucuacag cgacaacaac aagcgggacu ucaagacgau cccccucauc     4200

cuggccaucc agcgcgagaa ggagggccug aaccccugcc ccgacaccga cgagaagggg     4260

aaccagcucc uguucucccu gagcccgaac gagcuggugu ucgugcccaa caacgaggag     4320

cggaagaacc ccaaccucgu cgacuucgag aaccugagca aggaccagau cgaccgcauc     4380

uacaaguucg uguccugcac cggcagcgag gccuacuucg ugcccucccg gaccgccaag     4440

ccgaucaucg agaacgagaa cggcacgaac aacaagagcc agcgcaucau cgaguucaac     4500

gagaagaccu ccaucaagaa cgagaagaac gagcccgucc ugaucaagac caucugcugg     4560

aagaucgagg ugaaccggcu cgggaagauc aucaagguga ucaaguga                  4608


<210>  2288
<211>  4608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2288
augaccaaga uccugggccu cgaccugggg accaacagca ucggcugggc cauccgcgac       60

acggagaacg aggggaucaa ccagauccug gacaagggcg cccggaucuu cuccgagggg      120

gucaagagcg agaacggcaa ggagaucucc cgcgcggccg agcggaccgc cuaccgcagc      180

gcccggaaga ucaaguaccg ccggaagcuc cgcaaguacg agacccugaa ggugcugucc      240

aucaacggca ugugcccccu cagcguggag gagguggagc aguggaagaa guccggguuc      300

aaggaguacc cgcugaaccc cgaguuccug gacuggcucc ggaccaacga ggacaagaac      360

aucaaccccu accuguuccg cgacaaggcc agcaagcaga aggucacgcu guucgagcuc      420

ggccgggcgc uguaccacau cgcccagcgc cggggguucc uguccaaccg ccucgaccag      480

agcgccgagg gcguguucga ggagcacaac ccccagaucc agaaccugau cgaggaccug      540

gacuccagca acaccauccu caacgagcug aaggaguacu acaucaaccu ggggaucauc      600

gacgagaccg agaaguccgg cuucaagaag gaccucgacg agggcgagaa gaagcucaag      660

agccuguaca acucccuggu ggccaucacc aagaagaacg cgaacgacau cgagacgugc      720

aagcaggagc ugaucgcccg gcugaacaag aaggaggacc ucgggaaggu caagggcaag      780

aucaaggaca ucagccaggc caugcuggac cgcaacuuca agacccuggg gcaguacuuc      840

uucucccucu acaacaagga gcggauccgc aaccaguaca ccagccggga ggagcacuac      900

cuggaggagu ucaucaucau cugccagacc cagggcaucg agggcaucaa cacgaacgag      960

aagcugccgg agaagaaguu caccgggcuc gccaaggacc uguaccgcgc caucuucuuc     1020

cagcggcccc ugaaguccca gaagggccuc aucgggaagu gcagcuucga gaagaacaag     1080

agccgcugcg cgguguccca ccccgacuac gaggaguucc gcauguggag cuaccugaac     1140

accaucaaga ucggcaccca guccgagaag acgcugcggu uccucacccu ggaggagaag     1200

cugaagcucg ugcccaaguu cuaccgcaag agcgacuuca acuucgaggu ccuggccaag     1260

gagcuggugg agaagggggc cuccuucggc uacuacaaga gcuccaagaa gaacgaguuc     1320

uucuacuggu ucaacuacaa gccgaccgac agcguguccg ccugcgucgu gagcgccucc     1380

cucgagaacg cgaucggcaa ggacuggaag aucaagaccu ucgaguacca gacgcggaac     1440

accgagaaga acgaggugac caagagcguc gacuacaagg accuguggca ccuccugucc     1500

guggccacca gcgacacgua ccuguacgac uucgccaucg agaagcugaa gcuggagccc     1560

aagaacgcca aggccuucuc caagaccaag cucaagaagg acuucgcgag ccugucccug     1620

gccgccaucg ccaagauccu ccccuaccug aagcaggggc ugcucuacag ccacgccgug     1680

uucauggcga acaucgagaa caucgucgac gccgacaucu ggaaggacga ggagcagcag     1740

aaguucaucc aguccaagau cguggagcug guggacaacu acaucgucga gaaguccaag     1800

cuggagcuca ucaacggccu gcugaagauc uacaacaccg aggacaagga ggggcgcaag     1860

guguacuaca gcaaggaggc cgagucccuc uucgaggccg accugcggaa gaagcuggug     1920

cccuucuaca aggcgaacau caucaucgag gagcacgagc aggagaucau cuuccaggac     1980

cucuucccga uccugaugga ccagcugaag aagcaggagu ucaucaagau cggccgccuc     2040

gacaagcaga ucgaggccuu ccuggagggg gagaacgagg agggccagau cuucugcaac     2100

cacaccgaca agcugaagaa gcucuaccac cccagcgaca ucgaggucuu caagaagaag     2160

acgaucaagg acgagugggg caacgagaag gugguccugg ggagcccccu gaccaccagc     2220

aucaagaacc ccauggccau gcgggcccuc caccagcugc gcaaggugcu gaacacccuc     2280

aucgccaacg accagaucga cgaggacacg cggauccaca ucgagauggc gcgcgagcug     2340

aacgacgcca acaagcggaa gggcauccag gacuuccaga acgagaacaa gaaguuccgc     2400

gaggaggcca ucaaggagau caagaagcug uaccucgagg agugccacaa ggacgugaac     2460

ccgaccgagg acgacauccu gcgguaccag cuguggcucg agcaggggaa gugcgagauc     2520

uacgaggagg gcaacaacau cuccaucugc gacaucaucg ggagcaaccc cuccuacgac     2580

aucgagcaca ccaucccccg cagcaucucc caggacaaca gccagaugaa caagacccug     2640

ugcucccuga aguucaaccg gagcaucaag aagcagaaga ugcccgucga gcucuccaac     2700

uacaacgaca uccugccgcg caucgcccac uggaagaagg aggccgagga gcugacgcgg     2760

cagaucgaga ccaucagccg cuccaucaag agcgcggcca ccaagguggc caaggacaag     2820

aacauccgga agcgccacua ccucacccug aagcgcgacu acauccuggg caaguacgag     2880

cgguucaccu gggaggagcc caaggugggc uucaagaacu cccagauccc cgacacgggg     2940

aucaucacca aguacgccca ggccuaccuc aagagcuacu ucaagcgcgu cgagagcgug     3000

aagggcgggg cgguggccga guuccggaag cuguggggca uccaggaguc cuucaucgac     3060

gagaacuggu ggaagcacua caaggacaag gaccgcgaca agcacaccca ccacaccauc     3120

gacgccauca cgaucgccug caugcccaag gacaaguacg accugcucgc gcacgccugg     3180

cggcuggagg acgagcagga caagaaggcc gccaaggucc ugaucgagca ggccaagccg     3240

uggaagaccu ucaaggagga caucgagaag aucgagaccg agauccucgu gagccacuuc     3300

acccccgaca acgugaagaa gcaguccaag agcaucauca agaaccgcgg caagaagguc     3360

uacgugcuga agaacgagcu gcccgugaac uucaagaaca agaucgaggg gaaggacuac     3420

uucaagcuca aguucgacuc caagauccug uacaagaucc ccaagaagaa ggagaagcag     3480

acggacaccu ucuacgagga gcugccgaag aacuaccuca acggcgucga ggggaaggac     3540

uacuucaaga ucaacaccac cggcaagacg uucuacaaga uccccaucuu caaccagggg     3600

gacaccaucc ggggcagccu gcaccaggag accaccuacg gcgcgaucaa gcugcccgac     3660

aucgacaucg agacgaagaa gccgcuccac accgacaagg gcggguucau ccugaagaag     3720

gacaucaagg ggaacgagau cguguucuuc gugguccgca aggagaucuc caagaucagc     3780

gagaacgacg ugcagaacau cguggacaac gucgugcgga agaagaucga gaacgccauc     3840

gccaacuccc ugaucaccuu caagaucgug aagaagaaga aggucgccgu gaucaaggag     3900

ggcgugacca ucuggaugcg cgagcccaac aucgagaagg ggaucgaggg cauccccauc     3960

aagaaggucc ggaucaucac gaacagcgug aagaacccca ucgagaucaa ggugcacucc     4020

ccgcucagca agucccgcca caagcacaag cagaaggucu acggccagaa cgacgagaac     4080

uacgccaugg cgcuguacga gcuggacggg aagcgggagu ucgagcucau caacaacuuc     4140

aaccuggcca agcugcucaa gcagagccag uccuacuacc cccugcacaa ggagaaggag     4200

aucaagggca agaagauccu ggugcccauc gagaagcgca acaacaagga cgugauccuc     4260

aagcgggggc agcaggucgu guucuacgac aagaccgugg agaaccccaa ggacaucagc     4320

gagaucaucg acuuccgcga gcggaucuac aucaucgagg gccugaccau ccagcgccag     4380

aaggacaaga agaccuccaa ggucaacgag uacgggauca uccagcugcg gcacuucaag     4440

gaggcccgca agagcgagga gaucuccaag gacaacuuca agccggacgg cgaguucaag     4500

aucaacgaga acaagcccac gcggaagaug aaccacaacc aguucaccgc cuucguggag     4560

ggcaucgacu ucaaggugcu ccccagcggg aaguuccaga agaucuga                  4608


<210>  2289
<211>  4614
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2289
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccugaccacg       60

aacgacuucg acaccaagaa ggggaccauc gagggccucg ggucccgcau cauccccaug      120

agccaggaca uccugggcaa guucgacucc ggcgugagcc ugucccagac cgcccagcgg      180

acggaguacc gcgggaagcg gcgccucguc cagcgguucc ugcugcgccg ggagcgccuc      240

caccgggugc ugcacauccu ggagcuccug ccgaagcagu acagcgaggc gaucgacuuc      300

gacaagaagc ugggccaguu caagcccgag cgcgagcaga agaucuccuu cuacaagaac      360

gagagcggga agaaccaguu caucuucacc aagucccacc aggagaugcu caccgaguuc      420

cagaccuacu accccacgau caagaacauc cccuacgacu ggaccaucua cuaccugcgg      480

aagaaggccc ugaccgagaa gaucagcaag gaggagcucg ccuggauccu gcugaacuuc      540

aaccagaagc gcggcuacua ccagcuccgc ggggaggaga aggaggagaa caagaacaag      600

aaggaggagu uccacacccu gcuggugacg gacaucaucg agcgggacaa gggcaagucc      660

ggcaccuggu acaacgucau ccucgagaac ggguggaucu acaagcggga gagcaagauc      720

ucccuggccg acuggaucgg caagaccaag agcuucaucg ugaccacgga guacaacgac      780

gacggguccg ugaagcugac caaggacaac gaggagaagc gcagcuuccg guccccgaag      840

gaggacgacu ggggccucau caagauccgc accgagaaga ccaucaacca gagcgacaag      900

uccgucggcg ccuacaucua cgacgcgcug cugaagaacc ccaaccagaa gaucaagggg      960

gagcucgugc ggacgaucga gcgcaaguac uacaaggacg agcugcggga gauccugaag     1020

aagcagcucg aguuccacag cgacgugcug aacgacaaga uccuguacaa gaagugcauc     1080

gacgagcucu acaagaacaa cgacagccac aagaacucca ucaaggaccg cggcuucgac     1140

uaccuguucc uggacgacau caucuucuac cagcgccccc ucaagagcaa gaccucccug     1200

aucagcgacu gcucccugga guuccggccc aucaagaaca agaacggggu ccucgugaag     1260

gacaccgacg gcaagcagau cgccaaggcc cugaagugca ucgcccgcag caacccgauc     1320

uuccaggagu uccggcugcu ccaguucgug aagaaccuga agaucuacaa gcgcgggguc     1380

guggacgacg uggaggucac caacgacuac cugaaguccg agaacgacgu ggagaagcug     1440

uucaacuggc ucaacgacaa ggcggagauc aaccagaagc aguuccuggu gaacuucaag     1500

cugaaggagg acaagcaccg guggaacuuc gucgaggaca agggcuaccc cugcaacgag     1560

acgcgcaacc aguuccucaa cgccaucgcc aagaucgacg uggacaagag cuucuucaac     1620

accaagaaca cccaggaccu guggcacauc cuguacuccg ugaccgacaa gaucgagauc     1680

acgaaggcca ucaccaccuu cgccaagcgg cacaaccucc ccaacgaguu caucaucaac     1740

uucagcaaga ucaagcccua caagaaggag uacggcuccu ucagcgagaa ggcgaucaag     1800

aagcugcugc cgcucaugcg cuucgggucc uacugggacg ccaagaacau cgacaucaag     1860

acccuggacc ggaucgacaa gauccagacg ggcgaguucg acgagaagau ccgcaaccgg     1920

guccgcgaga aggccaucga ccugcacaag aucgacgacu ucaaggaccu cccccugugg     1980

cuggccagcu acaucgugua cgacaagcac uccgaggcca gcgacguguc caaguggaag     2040

acccccgcgg acaucgagaa guuccucaag cacgaguuca agcagcacag ccugcggaac     2100

cccaucgucg agcaggucau caccgagacc cugcgcgugg ugaaggacau cuggcuccag     2160

uacggggagg gcaaggagaa cuucuucaac gagauccaca ucgagcuggg gcgggagaug     2220

aagaacgaca aggccacgcg cgagaagauc uccaagaagg ugagcgagaa cgagaacacc     2280

aaccugcgga ucaaggccau ccucaccgag cugaagaacg acggcgucag cgacauccgc     2340

ccguacuccc ccagccagca ggagauccug aagaucuacg aggagggcgu guacaacaac     2400

gagucccgga aggagaagcu cgaggaggug gacaagaucc gcaagaacgc caagcccacc     2460

accagcgaga ucaucaagua caagcugugg cuggagcagg gguacgccuc ccccuacacg     2520

ggcaagcuca ucccgaugag cgagcuguuc accaccaagu accagaucga gcacaucauc     2580

ccccaguccc gcuucuucga cgacagccug uccaacaagg ucaucugcga gagcgaggug     2640

aacgagcuca aguccaacau gaccgcgcug gaguucaucc ugacgaacga gaaccggauc     2700

gugacccuca ccgggggcga gaccgucaag aucuucacga aggaggccua caaccagcac     2760

aucaucagcu acuacuccaa gaacaagaac aagcagaagc gccugcugac cgaggagauc     2820

cccgagcagu ucaucagccg gcagcucaac gacaccaagu acaucuccaa gaucgugaag     2880

aaccugcuga gcaagaucgu gcgcgaggag gacgagcagg aggucaccuc caagcacgug     2940

gugagccuca acgggagcau cacgucccgg augaagcagg acuggggccu gaacgacguc     3000

uggaacacca ucgugagccc ccgcuucgag cggcugaaca cccucaccaa cuccaacgac     3060

uucggccagu ucgagcugaa gaaggacgcc uacgggaacg agggcaagcg cguguuccag     3120

acgaccgucc cggacgccau cgccaagggg uucaccaaga agcggaucga ccaccgccac     3180

cacgcgcugg acgcccucgu gaucgccugc gugaaccgga cccacaucaa cuaccugaac     3240

aaccugaacg ccaaggacac ggacgacaag gcggucaagc acgagcuccg caacaagcug     3300

ugcuucaaga ccaagcccga cagcaacggc aacuacaagu gggaguucca caagcccugg     3360

gagaaguuca ccaaggaggc ccaggacaag cugaacacca cgaucguguc cuucaagcag     3420

aacacccggg ugaucaacaa gaccgucaac aaguacguga gcuacaagga cgagaacggc     3480

aaccucaacg uggacgccaa cgggcugccc cagaagaagg ucaucaccca gacgaagggc     3540

gacaacuggg ccauccgcaa gccgcugcac gccgagaccg uguccgggaa gguguuccuc     3600

aagcggauca agcagagcca caucaccauc gcgaacgcca ucgagcaggu cgagcugauc     3660

guggacaagg aggugaagaa gcagcugaag gccaagauca agcucuaccc caacaacauc     3720

gccggccuga agaagcaccu gaaggccuuc cccgucauga ucaacgacaa ggcgaucgac     3780

aagauccaga ucuacgagac ccucgaggcc acggccaccc gcaagauccu ggacaucacc     3840

uucgacgaga agaagaucca gaagaucacc gacacgggga uccagaagau ccugcucaac     3900

caccugaagc aggagaagua ccagaacacc guggacgaga acggcaagaa gcuggccccg     3960

cacgagaucg ccuucuccga gaacggccuc gaggaccuga acaagaacau caccuuccug     4020

aacgacggga agaaccacca gcccaucaag aaggugcgga ucuucgagga gggcaacaag     4080

uucccccucg gggaggucgg caacaagauc aacaaguucg uggagaccgc gaaggggacc     4140

aaccuguucu ucgccgugua cgagaacgac gacaagaagc gcaacuacaa gacgaucccc     4200

uucaacgagg ucaucgagcg gcagaagcag ggccugaacg ccgcccucga gaucgacgag     4260

aacggcaacc gccugcuguu cacccucagc ccgaacgacc ugguguacau cccccuggag     4320

gacgagaucg gguaccucaa cggcguguac uucaagaacc ugaacaagga gcagaccguc     4380

cggaucuaca aguucguguc cugcaccggg ggcgaggggc acuucgugcc caacaacuac     4440

agcaaggaga ucaucuccaa cgagaacggc agcaacaaca agaacgagcg caugcugaac     4500

uucaacaacu ucaacaucau cuucgacggc aaggagaagc ccgucaugau caaguccauc     4560

ugcuggaagc ucgaggugga ccgccugggg aaggugauca agaucaucaa guga           4614


<210>  2290
<211>  4617
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2290
augaagcaca uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaag       60

uccgagcagg acugcgacga cgucgagcgc aucaacggga ucgaggccgc gggcagccgg      120

aucaucccca uggacgccgc cgugcucggg gacuucgcca agggcaacac caagucccag      180

acggccgacc gcaccgcggc ccggggcauc cgccggaugg gggagcgcca caagcugcgg      240

cgcgagcggc ugaaccgcgu gcucgacguc augggcuucc ugccggagca cuacagcgcc      300

gcccugaccc gguacgggaa guucaaggac ggcgaggagu gcaagaucgc guggcgcaag      360

gacgaguccg ggcaguggac cuucguguuc gaggacagcu uccgggagau gcucgccgac      420

uucguggccc accagcccga guggcuggcc ggcggccgca agguccccua cgacuggacg      480

aucuacuacc ugcggaagaa ggcccucucc aagccccuga ccaagcagga gcuggcgugg      540

cuccugcuga acuucaacca gaagcgcggg uacuaccagc uccggggcga ggacgagcac      600

accgacgaca aggggaagcu gguggaguac uacgcccuga agguggucgg cguggaggac      660

accggcgaca agaaggggaa ggacacgugg uucaacauca ugcucgagaa cggcauggug      720

uaccgccggc cggccgccga ggcccccgac uggaucggga agaccaagga guucaucguc      780

accaccgacc uggagaagga cggcacgccc aagaaggaca aggaggggaa caucaagcgc      840

agcuuccgga ugcccaagga ggacgacugg acccugauca agaagaagac cgagaacgac      900

aucgaccgcu ccggcaagac cgugggcgag uacaucuacg acgcgcuccu ggccgacccg      960

acgcagaaga uccgcgggaa gcuggugcgg accgucgagc gcaaguacua caaggaggag     1020

cucaagaaga ucaucaacag ccagaagcgg uucauccccg agcugaacga caacgagcug     1080

uacggccgcu gcaucuccga gcucuaccag agcaacgacg ccuaccggca guccaucagc     1140

uccugcgacu ucaccuaccu guucguggac aacaucaucu ucuaccagcg cccccucaag     1200

agcaagaagu cccugaucga caacugcccc uacgaggagc acagcuacac cgacuccaag     1260

acgggggagg ugaagaccgc cccggucaag ugcguggcca agagcaaccc ccuguuccag     1320

gaguuccggc uguggcaguu cgugcagaac auccgcaucu ucgagcggca gaagaagauc     1380

ggcgacagcg ucaagaccga cguggacgug accggggagu ucaucaaguc cgcggacgac     1440

gucgugagcc ucuucgacuu ccugaacgac cgcaaggaca ucacgcaguc cguccugcuc     1500

ggcaccuacu ucaagaucaa gaagcccaag ggcaaggaca gcgacuaccc cuaccggugg     1560

aacuacgugg aggacaagac cuacccgugc aacgagaccc gcgcccugau ccugaagggg     1620

cucaaggacg ccggcaucga cgagggguuc cuggacaagg acaaggagua cucccugugg     1680

cacauccucu acagcgugga ggacaagcag gagaucacga aggccaugga gaaguucgcc     1740

gcgaagaacu cccugggcgg gaaguucgcc gaggucuuca gcucccugcc gcccuucaac     1800

aagaacuacg gcagcuacuc cgagaaggcc aucaagaagc uccuggcccu gaugcggacc     1860

ggcaaguacu ggagcgcgga cgccaucgac aagcacaccc uggagcgcau cgacaagauc     1920

aucaacgggg aguacgacga guccauccgg acccgcgugc gggagaaggc caucaaccuc     1980

acggacguga gccacuuccg cggccugccc cuguggcucg ccugcuacau cgucuacgac     2040

cggcacuccg agagcaagga caucacgugc uggaacugcc ccgccgacau cgaccaguac     2100

cuggaccacu ucaagcagca cucccugcgc aaccccaucg uggagcaggu cgugaccgag     2160

acccuccgca ccgugcggga caucuggcgc caguacggga agaucgacga gauccacauc     2220

gagaugggcc gggacaugaa gaaccccgcg gacgugcgcg ccaagaugag caagcggaug     2280

aucgacaacg agaacgccaa ccugcgcauc aaggccaugc uggccgaguu cgcggacccc     2340

gacuucggca ucgccggggu ccggccguac ucccccagcc agcaggagau gcuccgcauc     2400

uacgaggagg gcgcccugga gagcguggac gaggugcccg acgagaucaa cgagaucauc     2460

uccaaguuca accagaccga caucaagaag cggcccaccc acggggagau caucaaguac     2520

aagcuguggc ucgagcagaa guaccagagc ccguacaccg gccgcaccau cucccugagc     2580

cggcuguuca cggacgacua ccagaucgag cacgucaucc cccagucccg cuacuucgac     2640

gacagcaugu ccaacaaggu gaucugcgag gccgagguga accagcucaa ggaccggaug     2700

cugggguacg aguucaucaa ggcccaccac ggcgagaagg uccugcucag cuccggcgag     2760

accgugacca uccugaccgc ggaggaguac gagcgccugg ugcagcucaa cuuccggaag     2820

aaccccguca agaugaagaa gcugcugaug gacgacaucc cggacaaguu caucgagcgc     2880

cagcucaacg acagccggua caucuccaag aagaucaaga gccugcuguc caacauggug     2940

cgcgacgagg gggagcagga ggacaucagc aagaacguga ucgccugcaa cggcgccauc     3000

acggaccggc ucaagcgcga cuggggcguc aacgacgugu ggaaccacau cauccugccc     3060

cgguucgagc gccugaacgc cgugaccggg uccgaccgcu ucaccaccac gagcaccaac     3120

gggcacacca uccccgacau gccccucaac cugcagaagg gcuucaacaa gaagcggauc     3180

gaccaccgcc accacgcgau ggacgccauc gucaucgccu gcaccacgcg gucccacgug     3240

aaccugcuca acaacgaggc cgccaugagc aagaacaacg cggugcgcua ccagcugagc     3300

cggaagcugc gccgguacga ggacaccaug gucacccgca acggcgagac ccggacgauc     3360

uccguggccc gcgaguuccu caagccgugg cccaccuuca acgccgacgu ggagaaggcc     3420

cugcggggga ucgucgugag cuucaagcag aaccugcgcg ugaucaacaa gaccgucaac     3480

aaguaccaga aguucgugua caacgaggag aagcacauca aggagaagaa ggccgugcgg     3540

caggaggagc gcaacggccg gcaccuccug gcgauccgca agcccaugca caaggacacc     3600

gucuacgggg aggugaaccu gcggcgcauc aaggaggugu cccucaagga cgccgucgcc     3660

aaccccaagg ccguggugga caaggaccug aagaagaagc ugauggccau gaucgagcuc     3720

ggcuacgacc ugaagcagau cacgaaguac uucgaggaca acaaggacgc guggcaggac     3780

gucaaccuga agaagaucaa gguguacuac uucaccaagg acaccgggga ccgguacuuc     3840

gccacccgcu ucggcaacga ccucgugacg cuguucagca agaucaccaa ggccgacgac     3900

gccaagaagc agaucgagcg caucaccgac accggcaucc agaagauccu gaagcggcac     3960

cucgaggcga acgggggcga cugccagaag gccuucuccg ccgacgggau cgacgagaug     4020

aaccgcaaca ucacgcagcu gaacggcggg cggccgcaca agcccgucua caaggugcgc     4080

aucuacgaga aggccgacaa guucgccauc ggcaccaagg gcaacaaggc ggccaaguuc     4140

guggaggccg ccaaggggac caaccuguuc uucgccgucu accucaagga guacgaggac     4200

agcaccacgg gcgagaccau caagaagcgg guguucgaca ccaucccccu gaagaccguc     4260

aucgaccgcc ugaagaacgg gcagacgccc gcgccggagg agaaggacgg cgccaagcuc     4320

cuguucgugc ugucccccaa cgaccucgug uaccugccca ccgaggacgu cgugaagaac     4380

ggcaccauca accggccccu ggacaagcgc cggaucuaca agauggugag cugcaccggg     4440

accgagugcu acuacauccc guucaucguc gccuccccca ucguggacaa gguggaguuc     4500

uucagccuca acaagaucgg ccgcgccauc acgggggaga ugaucaagga gaucugcauc     4560

cccaucaagg ucgaccggcu gggcaacauc accgagguga acaacgagaa gaucuga        4617


<210>  2291
<211>  4623
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2291
augaacaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugggg       60

accgacgagg agggcaaccc cauccgcauc uacaaguccg ggagccggau caucccgaug      120

ucccaggacg uccucggcaa cuucgaccgc ggcaacagca ucucccagac ggccgagcgg      180

accagcuacc gcggggugcg gcgccugcgg gagcgccacc uccugcggcg cgagcggcug      240

caccgcgugc ugcaccucau gggcuuccug cccucccacu acgaggaggc gaucgggugg      300

gacaagggcg acaacaagac cuucgccaag uucuacagcg acaccgagcc caagcugccc      360

uggcggaaga acgccgagcg cgagcacgag uuccucuuca acacgccgua caacgagaug      420

cuggucgacu uccggaagaa ccagccccag cuguucaaga ucaagaagaa cgggcaggag      480

ucccucaucc ccuacgacug gaccaucuac uaccugcgca agaaggcccu gagcaagccc      540

aucuccaaga aggagcucgc cuggaucauc cugaacuuca accagaagcg gggcuacuac      600

cagcugcgcg gcgaggagga ggaggagaac agcaacaagc ucaucgagua ccacgcgcug      660

accgugguga aggucaucgc cgacgagccg cagaagggga aguccgacau cugguacaac      720

gccgagcugg agaacggcug gguguaccgg cgcagcucca agacgccccu cuucgacugg      780

gagagcaagg ugaaggaguu cgucgugacc accgaccuga acgccgacgg gucccugaag      840

guggacaagg agggcggcgu caagcggagc uuccgcgugc ccuccgagga cgacuggaug      900

cucgugaaga agaagaccga gagcgacauc gagaaguccg acaagaccgu cgggugcuac      960

aucuacgaca cgauccugca gaaccccucc cagaagaucc gcggcaagcu ggugcggacc     1020

aucgagcgca aguucuacaa gaaggagcuc accgagaucc ugaacaagca gaaggaguuc     1080

cacagcgagc ugcagaacaa ggagcucuac caggagugca ucaaggagcu guacccgcac     1140

aacgaggcgc acuacgacuc ccugcucgug aaggacuaca ccgaccuguu cgucaacgac     1200

aucaucuucu accagcggcc ccucaagagc aagaaguccc ugaucagcaa cugccccuac     1260

gaguaccgcg uguaccggaa cgacuccggg aucguggagc gcguccccau caagugcauc     1320

gccaagagcc acccgcugua ccaggaguuc cggcuguggc aguucaucca caaccuccgc     1380

aucuacgagc ggcagcgcga ggugaacggc aagcccgaga cggacgugga caucaccgcc     1440

gaguucaucc ccaccaccga ggaccggguc aagcuguucg acuggcugaa caacaagaag     1500

gagaucaagc aggacacgcu ccugcagucc uacuucaaga ucaagaagaa gaagggggac     1560

gacaaguucc ccuaccgcug gaacuacgug gaggacaagg aguacccgug caacgagacc     1620

cgggcccuga uccucagccg ccugaccaag cugaaccugu ccgaggacuu ccucaccaag     1680

gagauggagc uggcccugug gcacauccuc uacagcgucg aggacaagga ggagaucaag     1740

aaggcguuca aguccuucgc ccagaagcac aaccuggacg acagcuucgu ggaccaguuc     1800

aucaaguucc cgcccuucaa gaaggacuac ggcuccuaca gcgccaaggc caucaagaag     1860

cugcuccccc ugaugcggau cggcaaguac ugggacgaga aggccaucuc cgggaacacg     1920

cgcagccgga uccuguccau caacgagcgc cucgaggaca ugcggagcag cggcaagaag     1980

aucgaggacg uguccgacga cgacaucccc aagcaggucc ucaagagcuu cgacaaggug     2040

aacggggugu acuccggccu gaacaccuac caggcgugcu acgccgucua cgggcgccac     2100

agcgaggccu ccgagaucac caaguggaag acgccccagg acaucgacga cuaccugaac     2160

gaguucaagc agcacagccu gcgcaacccc aucguggagc aggucaucac cgagacccug     2220

cggguggugc gcgacaucug gaagaaguac ggcgacuucu ccgagaucca caucgagcuc     2280

ggccgggaga ugaagaaccc cgccgacaag cgcgccaaga ugaccaccca gaucagcgag     2340

aacgagaaca cgaaccugcg gaugaagucc cugcucgcgg agcugaagaa ccacaacgac     2400

guggagaacg uccgcccgua cagccccucc cagcaggaga uccugaagau cuacgaggag     2460

ggggugcuca acagcguguc cgagaucccc gaggagauca ucaagaucac caagagcucc     2520

cagccgagcc agaccgagcu gguccgguac aagcuguggc ucgagcagaa guaccgcucc     2580

cccuacaccg gcgagaucau cccccugggg aagcuguuca cgagcgccua cgagaucgag     2640

cacgugaucc cccagagccg guacuucgac gacucccuca gcaacaaggu gaucugcgag     2700

uccgagguca acaagcugaa gggcaaccug cucggguacg aguucgugaa gaaccaccac     2760

ggcgugaagg ucgagcugaa cuucaacaag accguggaga ucuucagcau caacgaguac     2820

gaggaguucg ugaagggcaa cuacgggaag aaccgcggca agaugaagaa gcugcuccug     2880

gaggacaucc cgaaguccuu caucgagcgg cagcugaacg acacccgcua caucagcaag     2940

gucgugaagg ggcuccuguc caacaucgug cgggaggacg gcgagcagga ggccaccagc     3000

aagcacguca uccccugcac cggcggggug acgaccaucc ugaagcagga cuggggccuc     3060

aacgacgugu ggaacaagau cgucacgccc cgcuucgagc ggaugaacga gcugaccaac     3120

accucccugu ucgggcagug ggagaacaag gagggcaagc gcaucuuccg gaccgaggug     3180

ccccucgagc ugcagaaggg guucaacaag aagcgcaucg accaccggca ccacgccaug     3240

gacgccaucg ugaucgcgug cgccacccgc agccacguca ccuaccugaa caacgagucc     3300

agcaaggcca acgccaagga gauccgggac ggccucaagc acaagcugug cuucaagucc     3360

aagacggacg agaccggcaa cuacaagugg caguuccaca agccguggga caccuucacc     3420

caggacgugc aggacacgcu ggagaacauc gugaucagcu ucaagcagaa ccuccgcguc     3480

aucaacaaga ccaccaaccg cuaccagaag uacgaggagg agaagaagau ccugaagucc     3540

caggagaagg gggacagcug ggcgauccgg aagccccugc acaaggacac cguguucgcc     3600

aagguguccc uccagaagau caagugcguc aagcuggcca uggcccugaa ggacuggaag     3660

aacaucgugg acaagcagcu caagaaggag auccgccggc ugauccugag cuacggcaag     3720

uucgacgccg agauccucaa caaguacuuc aaggaccgca aguacaucuu caaggacgug     3780

gacaucucca aggucgacgu guacuacuuc gagacggagu acguggcgac ccggaagaac     3840

cuggacacca gcuucgaccg caagagcauc gaguccauca ccgacacggg gauccagaag     3900

auccugcuca accaccuggg cgucaagaac aacaaccccg agcuggccuu cagcgccgag     3960

gggaucgacg agaugaacca gcggaucgug gagcucaacg gcggcaagca gcaccagccc     4020

aucuacaagg ugcgcaugua cgagccgcug gggaacaagu uccaggucgg ccggaucggg     4080

aacaagaagg acaaguacgu ggaggccgcc cccggcacca accuguucua cgcgaucuac     4140

caguccgagg acaagaagcg caucuacgac accauccccc ucaacaucau caucgagcgg     4200

cagaagaagg agcuguuccc cgugccggag aacaaggagg acaaggacaa ccuguacaag     4260

cuccuguucu ggcugagccc caacgacaug gucuaccucc ccaccgagaa ggagugggcc     4320

aaccccgacc uguucaacuu caacaacaug acgaagaacc agcgcaagaa gcuguacaag     4380

uucaucgacg gguccggcac caccgccaac uucaucccga acaccguggc caacaucauc     4440

uucagcaucc ccaagaagga cuccggcaag uacagcaugg ggaaccggau cguccagaac     4500

gaguucggca ucgggagccc ccagagcaag aaccagaagg ccuucacggg cgagaugauc     4560

aaggagcgcu gcuggaagcu caagaucaac cggcuggggg acaucaucga gugcaucaag     4620

uga                                                                   4623


<210>  2292
<211>  4629
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2292
auggagaagc gcauccuggg ccucgaccug gggaccaaca gcaucggcug ggcccuggug       60

aagggggagc ggcacgagaa cgacaacgag accauccucu ccuacaucga ggccgcgggc      120

agccgcaucc ugccccugga cgccgccgcc cagggggacu ucgagaaggg caacccggcc      180

aagacgcaga ccuccgagcg gacccgcaug cggggcaugc gccaccuccu ggagcggagc      240

cugcuccgcc gggagcgccu gcaccggguc cuggacauca uggacuuccu cccgccccac      300

uacucccaga gccugacccg cuacgggaag uucaaggcgg acacggagug caagcuggcc      360

uggcggaaga ccgagaccgg ccacuacgag uuccucuucc aggacuccuu ccaggagaug      420

cugcaggacu uccagaacca cgggaagcug gaggagaaca agaagauccc cuacgacugg      480

accaucuacu accuccgcaa gaaggcccug acgcagcccg ugagcaagca ggagcuggcc      540

uggauccucc ugaacuucaa ccagaagcgg ggcuacuacc agcgcgagga ccaggcggug      600

gacgacaacc agaagaagcg ggucgaguac caggcccuga aggugaucuc cguggaggac      660

accggggaga agaagggcaa gaacaccugg uacgacgucc gccucgagaa cggccuggug      720

uaccacuaca gcgcccccau caagcccgac uggaaggggg agaccaagga guucaucgug      780

acgaccacca ucgaccagaa cggcaagccc gagcuggaca aggacgggaa gccgaaguac      840

uccaugcggg cccccaagga ggacgacuac aagcuccuga uggagaagac caagaaggac      900

aucacggaca gcggcaaguc cgucggcgag uacaucuacg acgcccugcu cgugaacccc      960

cagcagaaga uccgcggggg ccuggugcgg accaucgagc gcuacuucua caagaaggag     1020

cuggagaaga uccuccugag ccaggagcgc uucauccccg agcugaccga ccgggagcuc     1080

uaccgccggu gcgucgagga gcuguacccg uccaacgacg cguaccgcca ccugaucgag     1140

agccgggaca ugcgcuaccu ccugguggag gacauccugu ucuaccagcg gccccucaag     1200

uccaagaagu cccugaucga cgacugcccc uacgagagcc gcagcuacgu ggaccgggag     1260

accggggaga ucaagagcuc ccccgucaag ugcaucgccc agagccaccc gcuguuccag     1320

gaguuccgcc ucuggcaguu cauccacaac cugcggaucu accagaacga gcgcuccgug     1380

gagggcaagc ugcagcucga cguggacguc acggaggagg ugcugcccac ccccgagagc     1440

aaggccgccc uguuccgguu ccugaacgac cgcgagaacg ugacccagga gauccuccug     1500

aaggaggucu ucaaggggcg gaagcugucc gugaaggacu gccccuaccg cuggaacuac     1560

gccggcgagg gcaagaagua cccgugcaac gagacccggc accucaugcu gaagcugcuc     1620

aagaaggcgg ggaucgacac ggccuuccug acccccgaga aggagcaggc ccuguggcac     1680

aucauguaca gcguggagga caaggaccag uacgagaagg cccucuccac cuucgcccgc     1740

aagaacgacc ugcccgacgg cuucaaggag caguucaaga aggcgcccac cuucaagaag     1800

gacuacgggg ccuacagccu gcgggccauc aagaagcucc ugccgcugau gcgcgucggc     1860

gaguacuggu ccgccgacug caucgacagc gagacgaagg agcgcaucuc ccagaucauc     1920

cgcggggaga uggacgagaa ggugagccag cggaugacca agucccggga cagcuucacc     1980

gacaucuccc aguuccaggg ccuccccguc uggcaggccu gcuacguggu guacggccgc     2040

cacagcgagg cgaccgagau ccagcggugg gagaagcccg aggacaucga cgucuaccug     2100

aaggccuuca agcagcacuc ccugcgcaac cccaucgugg agcaggucgu gcucgagacg     2160

cugcgggugg ugcgcgacau cuggaagacc uacgggaagg ucgacgagau ccacaucgag     2220

augggccggg acaugaagaa caccgccgag gagcgcaagc ggaucuucaa ccgcaaccag     2280

cagaacgaga acgacaaccu gcggaucaag gcccuccuga ccgaguucau gaacccggac     2340

uucgagaucg agggggugcg ccccuacagc cccucccagc aggaggcgcu gcggaucuac     2400

gaggacggcg ccuggagcgg gcucgacgag gagaaggagg acaagaagga gcuggaggcc     2460

auccgcgaga uccugggcaa guucagccag uccgacaagg ccaagcggcc gacgcacagc     2520

gaggugcucc gcuacaaggc cuggcuggag cagcgguacu ccagccccua caccggcgag     2580

gcgauccccc uggccaagcu guucaccccc gccuacgaga ucgagcacgu caucccgcag     2640

ucccgcuucu ucgacgacag cuucuccaac aagguggugu gcgaggccga ggucaacaag     2700

cucaaggacc ggaugcuggg guacgaguuc aucaaggccc accagggcga gguggugagc     2760

cucuccggga ccggccgcug cgcgcgcauc cugagccccg aggaguacga gcagcggguc     2820

accgacugcu acaagcacaa cacggccaag augaagaagc ugcucaugga ggagaucccc     2880

gacgacuuca uccagcgcca gaugaacgac ucccgguaca ucagcaagcu ggugaagucc     2940

cugcucagca acauggugcg cgaggagggc gagcgggagg ccaucuccaa gcacgucauc     3000

acgugcaacg ggaccaucac cgaccgccug aagaaggacu ggggcgugaa cgacgugugg     3060

aaccacauca uccugccccg guuccagcgc augaaggacc ucaccgggcg gaacgacuuc     3120

gucgccguga acagccaggg ccacgagauc ccguccgugc cgcccgagcu gcagaagggg     3180

uucaagaaga agcgcaucga ccaccggcac cacgccaugg acgcgaucgu caucgccugc     3240

accacccgca accacgugaa ccugcucaac aacgaggccg ccaugagcga gaacaaggcg     3300

aaccggaucg cccugagccg caagcugcgg cgcuacgaga acugcgccau cuacaagggc     3360

ggcgagacca agaacaucuc cguggccaag gaguuccuca agcccugggc cacguucccc     3420

gcggacgucg agcgggcccu gggggacauc guggugagcu ucaagcagaa ccugcgcguc     3480

aucaacaagg ccaccaacca cuacgagcac uacaaggacg gcaagaaggu gaaggugccc     3540

cagaagaagg gggacaugug ggucgugcgg aagccccucc acaaggacac cguguacgcc     3600

cuggucaacc ugcgcaacgu gaagaccgug ggccucaagg acgccauccu gaugcccgac     3660

cgcaucgcga acaaggaccu gaagaagaag gucaaggagc uccuggccca gggggacgac     3720

cugaagaaga ucaagaagua cuucgagacg gagaaggaca ccuggcagga cguggacgug     3780

aagaagaucg aggucuacua cuucuccgag gagaccgcca accacuacua cgccacccgg     3840

aagccguucg acgccaacgu gaagaucgac agcgugacgg acaccggcau ccagaagauc     3900

cuccgccggc accuggagga gaacggcaac aaggcggacg ucgccuucuc ccccgagggg     3960

aucgcccgca ugaacgagaa caucuuccgg cugaacggca acaagcacca ccagcccauc     4020

uacaaggugc gcguguacga gaaggccaac aaguaccccg ucgggcagac cggcaacaag     4080

gcgagcaagu ucguggaggc cgccaagggg accaaccucu accuggccgu cuacgagucc     4140

gagguggugg acaagaagac ggccgagauc agcaagaagc gguccuacgu caucgugccg     4200

cugcgcgacg cgguggcccg gcagaagcag ggcguccccg uggcccccga gcgcaacgag     4260

gagggcggga agcuccuguu cgugcugagc cccaaggacu ucgucuacgu gccgaccgag     4320

gaggagcggu ccaagggcga ggugcuccug ccccuggaca agaaccgcau cuacaucaug     4380

auggacgcca gcgacgggau cgucaacuuc cuccccuacu ccguggccaa ccugaucuac     4440

gcgcugccga aggaccaggc cagcggcuuc ugcaccgacg ggcggaugau ccagaacgag     4500

uucggccucg gcucccccaa gagcaagaac gagcgcgccc ugaccgggga gaugaucaag     4560

gagaucugcc ugcccgugaa ggucgaccgg cucggccgca ucacggagau caacgggaac     4620

aagguguga                                                             4629


<210>  2293
<211>  4644
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2293
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccugaccaac       60

aacgacuucg acaacaagcu cggguccauc gagggccugg ggagccgcau cauccccaug      120

ucccaggacg ugcugggcaa guucgacagc ggcgucucca ucagccagac ggccgagcgg      180

accaaguacc gcgggacccg gcgccucgug cagcgguucc ugcugcgccg ggagcgccuc      240

caccggaucc ugaacauccu ggacuuccuc ccgcgccacu acuccgaggc gaucgacuuc      300

gacaagaagu ucggccaguu caagcccgag cgggaggaga agcugagcuu cuacaagaac      360

gaguccggga agaaccaguu ccuguuccuc gagagccacc aggagauguu cuccgaguuc      420

aagcugaccc accccgaccu gaagcagguc cccuacgacu ggacgaucua cuaccuccgc      480

aagaaggccc ugacccagaa gaucagcaag gaggagcugg ccuggaucau ccucaacuuc      540

aaccagaagc ggggcuacua ccagcugcgc ggggaggagg aggaggugaa caagaacaag      600

cuggaggagu uccacacccu cgugaucgug gacgucaucg agcgggagaa gggcaagucc      660

ggcguguggu acaacgugau ccuggagaac ggguggaucu acaagcgcga gagcaagauc      720

gcccuguucg acuggaucgg caagcagaag aacuucaucg ucaccacgga gcucaacgac      780

gacgggacca ucaagcugaa caaggacaac gaggagaagc gguccuuccg cagcccgaag      840

gaggacgacu ggggccugcg gaagauccgc accgagcaga ccaucaacca guccgagaag      900

uccgugggca gcuucaucua cgacgagcuc cugaagaagc ccaaccagaa gaucaagggg      960

gagcuggugc ggacgaucga gcgcaaguuc uacaaggacg agcucagcaa gauccugaag     1020

gcccagauca ccuuccacua cgagauccug ggcgaccgca acaucuacaa gaagugcauc     1080

gacgagcucu accggcacaa cgacagccac aagaacaaca ucaaggacaa gggguucgac     1140

uaccuguucc uggacgacau caucuucuac cagcgccccc ucaaguccaa gaccagccug     1200

aucuccgacu gcagccugga guucgucccc aucaaggaca agaagggcau ccucgugaag     1260

gacuccaacg ggaagcaggu cgugaagccg cugaagugca ucgccaagag caaccccauc     1320

uuccaggagu uccggcugcu ccaguucgug aagaaccuga agaucuacaa gaaggagauc     1380

aucaacgaga ucgacgugac caacgacuac cugaagacgg aggaggaccu cgucgagcug     1440

uucgacuggc ugaacgacaa ggcggagauc ucccagaagc aguuccuggc cuaccccaag     1500

uucaagcuca aggaggacaa guaccgcugg aacuucgugg aggacaagau cuaccccugc     1560

aacgagaccc ggaaccaguu ccugaacgcc aucgcgaaga ucgacgugaa caagagcuuc     1620

uucgacgcca agaacaccca ggaccugugg cacauccucu acuccgucac cgacaagacg     1680

gagcuguuca gcaucaacac cgagaccggc auccaguccg gcgcccugag caccuucgcc     1740

aagcgccaca accucuccaa cgaguucgug agcaacuucu ccaagaucaa gccguacaac     1800

aaggacuacg ggagcuacuc cgagaaggcc aucaagaagc ugcugccccu caugcgguuc     1860

ggcagcaacu ggaacaagaa caacaucgac gcgaagacgc uggcccgcau cgacaagcug     1920

cagaccgggg aguucgacga gaccaugcgg acccgcgugc gggagaaggc cauccaccuc     1980

uccaagauca cggacuucca gggccugccc cuguggcucg ccagcuacau caucuacgac     2040

cgccacagcg aggccuccaa caucagcaag uggaagaccc ccgaggacau cgcgugguuc     2100

cugaacccga agaacaaguc cggguucaag cagcacagcc ugcggaaccc caucgucgag     2160

caggucauca ccgagacccu ccgcguggug aaggacaucu ggcagcacua cggcgagggc     2220

aaggagaacu ucuucaacga gauccacauc gagcuggggc gggagaugaa gaacaacgcc     2280

aacgaccgca agcggaucac ggagaccauc aaccagaacg agaacaccaa ccugcgcauc     2340

aaggccaucc ucaccgagcu gaagaacgac ggcaucaacg acgugcggcc cuacuccccc     2400

agccagcagg agauccugaa gaucuacgag gagggggucu acaacuccga gauccgcaag     2460

gagaagcucg aggacaucga caagauccgc aagaacgcca agccgacgcc cagcgagauc     2520

aucaaguaca agcuguggcu ggagcagggc uacaucuccc ccuacaccgg gaagcucauc     2580

ccccugagcg accuguucac caccaaguac cagaucgagc acaucauccc gcagucccgc     2640

cucuucgacg acagccuguc caacaaggug aucugcgaga gcgaggugaa cgaccugaag     2700

ggcaacaaca ccgcccucga guucauccug aagaacgagg gccggaucgu ccugcucacc     2760

gggggcaaga cggugaagau cuucaccaag gaggcguaca accagcacau caccuccuac     2820

uacaucaaga acaagagcaa gcagaagcgg cugcugacgg aggagauccc cgagaaguuc     2880

aucgaccgcc agcucaacga caccaaguac aucuccaaga ucgugaagaa ccugcugagc     2940

aagaucgucc gggaggagga cgagcaggag gugaccagca agcacaucgu gucccucaac     3000

gggagcauca ccucccgcau gaagcaggac uggggccuga acgacgucug gaacaagauc     3060

gugagccccc gguucgagcg ccugaacgcc aucacgaacu ccaacgacuu cggcaagcuc     3120

gagcugaaga aggacgaguu cgggaacgcc ggcaagcagg ucuuccagac caccgugccc     3180

gacgacaucg ccaagggguu cagcaagaag cggaucgacc accgccacca cgcccuggac     3240

gcgcucguga ucgccugcau caaccggacc cacaucaacu accugaacaa ccugaacgcc     3300

aaggacgaga acgacaagac gaucaagcac gagcuccgca acaagcugug cuucaagacc     3360

aagccggacu ccaacggcaa cagcaagugg gaguuccaca agcccuggga gaacuucacc     3420

aagaaggugg aggacgagcu gaaccagacc aucaucuccu ucaagcagaa cacgcggguc     3480

aucaacaaga ccgugaacaa guaccaggug uggaaggagg aggacggggc caugaagaag     3540

gucaccguga agcagaccaa gggcgacaac ugggcgaucc gcaagcccau ccacgccgag     3600

acggugagcg gcaaggucuu ccucaagcgg aucaagcagu cccacaucac caucgccaac     3660

gccaucgagc agaucgagcu gaucguggac aaggaggugc gcaagcagcu gaaggccaag     3720

aucaagcagu acccgaacaa caaggagggg uucaagaagc accucaaggc guuccccauc     3780

augaucaacg gcaaggccau cgacaagguc cagaucuacg agaccaucga ggccaccgcc     3840

acgcgggagc gcaacaaccu ggacaucacc uucgacgaga agaagaucga gaagaucacc     3900

gacaccggga uccagaagau ccugcucaac caccugaagc aggagaucua ccagaacgcc     3960

aucgacgaga gcggcaagaa gaucgcgccc cacgaggugg ccuucuccga gaucgggauc     4020

gacgagcuga acaagaacau caaggugcuc aacaacggca agaaccacca gcccaucaag     4080

aagguccgga ucuucgagga gagcaagcgc uucucccugg gccagagcgg guccaagacg     4140

gacaaguacg uggagaccgc caagggcacc aaccuguucu acgccaucua cgaggggaag     4200

aagggcaacc ggaacuacaa gaccaucccg cucaacgagg ugaucgagcg ccagaagcag     4260

gggcugaaca gcgcccuggu cacggacgag aacgagaacc gccuccuguu cacccugucc     4320

cccaacgacc ucguguacau ccccaccaac gaggagaaga agaaccugac cacgaucaac     4380

uucgacaagc ugagccggga gcagaccgag cgcaucuaca aguucgugag cugcaccucc     4440

ggcgagggcc acuucgucag caacaacuac uccaaggaga ucaucagcaa cgagaacggg     4500

uccaacaaca agaacgagcg gaugcucgag uucaacaaca gcaacaccau cuacgacgag     4560

aaggagaagc ccgugaugau caaguccgug ugcuggaagc uggaggucga ccgccugggc     4620

aagaucaagc ggaucauccg cuga                                            4644


<210>  2294
<211>  4677
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2294
augaagaaga uccugggccu cgaccugggg accaacagca ucggcugggc ccuggugaac       60

gucgacaaga acgggaaccc cuucggcauc gaggggcucg gcucccgcau caucccgaug      120

gaccaggcca cccugagcga cuucgagaag ggcaacucca agagccagac ggcggagcgg      180

accauguucc gcggggcccg gcgccugcgg gagcgcaagc accuccggcg cgagcggcug      240

caccgcaucc ugaacgagcu caacuuccug cccauccacu accgguccug caucgacuuc      300

gugcaccgcc ugggcaaguu caaggccaac cacgagccca agaucgccug gcggagcaac      360

gaccaggaca agauggaguu caucuuccag aacuccuucc ucgagaugau gagcgacuuc      420

accaagcgcc agcccgagcu ggugaaggag gggaagaaga ucccguacga cuggaccauc      480

uacuuccugc ggaagaaggc ccucggccag cgcaucgaga aggaggagcu ggcguggcug      540

cuccugcacu ucaaccagaa gcggggguac uaccagcugc gcggcgagga cgagaucgac      600

gccacgcggu ccgccaagac ccgcaaguac uucgaccgga agaucaucaa gagcaucauc      660

gacaccggcg aggaguacaa ggggcucaag guccugaucg uggagcugga caacggcaac      720

caggggaaga ucuucaagca ggaggugccc gacugggucg gccaggagaa gaacaucauc      780

gccaccgugg accucgacaa ggacggcaag gaccgcuucg acaagggggu gcugucccag      840

cgcuucagca uccccuccga cagcgagugg gagacggagu ggaagcugau caagcucaag      900

accgaggagg accuggaggc gucccagaag accgucggca ccuacaucua cgacagccug      960

cucaagaacc ccaaccagaa gaucaagggg aagcuggugc ggacgaucga gcgcaaguac     1020

uaccgggacg agcugcgcgc cauccucaac aagcagcggg aguuccaccc ggagcugaac     1080

gacaagaagc uguaccagca cugcaucuuc ucccucuacc cccagaacga ggccuuccgc     1140

aacagcaucg ccaacaagga cuucaccuac cuguuccugg aggacaucau cuucuaccag     1200

cggccccuca aguccaagaa guccaccauc aacaacugcg ccuacgaggu gcgccgguac     1260

auggacaagg agaccaagga gcugaaggag gucccccuga agugcaucgc caagagccac     1320

ccgaucuucc aggaguuccg ccucuggcag uucaucagca accugcggau cuacaagaag     1380

gacaucacgg acaucgacgu gaccaacgac uuccugcaca gccaggacga cuacgcgaac     1440

cucuucgagu ggcugaacaa caagaacgag aucgaccaga aggccuuccu gaaguacccc     1500

gccuucggcu ucaagaagga cgccgugaac uaccgcugga acuacgucga ggacaagucc     1560

uaccccugca acaagacccg gagcgcgauc cucuccaagc uggacaagca cgagcaggag     1620

uuccugacca aggagcugga gcucaagauc uggcagcugc uguacagcgu gaacuccaag     1680

gaggagaucg acaaggcccu caacgugaac agccccaaga ucaacaacaa gaucuccauc     1740

gaccacacgg ggauccugca cgagcucaag agcuccagcc ucuccgacau cagcauccag     1800

cgccugaagg ccaucaacuu cgaggagaag gacuacggcu ccuacagcua caaggccauc     1860

aagaagcugc ucgcccugau gcggcagggc aaguacuucg acaacaccgc gaucaccgac     1920

gccaccaagg cccgcaucaa cuccaucuuc gaccggcugg agagcaucuc cuucgacccg     1980

gagcgcauca acagcaucag cgacgacgac auccccaagc agauccucaa guccuucauc     2040

aagcugaacc ggacguacgg gggcuucaac accuaccagg ccugcuacgc cgucuacggg     2100

cgccacagcg aggcgaagga gaucgccaag ugggagaagc ccgaggacau cgaccaguac     2160

cuggccaagu ucaagcagca cucccuccgc aaccccaucg uggagcagau caucaccgag     2220

acccugcggg ugguccgcga caucuggaag aagcacgccu acaagacgau caacaaggac     2280

aaccagggca acaacugcga gagcgagucc gagcugaccg agauccacau cgagcucggg     2340

cgggacauga agaacaacaa gagcgagcgc cugcggaucu cccgcaucaa cagcgagaac     2400

gagaacacca accugcggau ccgcaagcuc cugaucgagu ucaacgaccc ggaguucggc     2460

guggagaacg ugcggcccca cucccccagc cagcaggaga uccugcgcau cuacgaggac     2520

ggcguccuca aguccggggu ggagaucccc gaguacgucg acaacaucau caagaaguuc     2580

aaggagaccg acgugaagaa gcagccgagc aaguccgagg ugcugcggua caagcugugg     2640

cucgagcaga aguaccgcag ccccuacacg aacgagguca ucccccuggg caagcuguuc     2700

accaacgacu accagaucga gcacaucauc ccccaguccc gguacuucga cgacagcuuc     2760

uccaacaagg ugaucugcga ggcggaggug aaccagcuca aggagaacga gcuggggugc     2820

gaguucauca ugaacaacgg cgggcgcauc gucgagcuga gcuucggcaa gaccgugcgg     2880

auccucgacu ucgaggccua cgagcaguuc gugcagcaga acuacgccgg caaccgcccg     2940

aagcugcgga accugcucau gaccgagauc cccaacgacu ucaucgaccg ccagcugaac     3000

gacagccggu acaucuccaa ggucgugaag gggcugcuca gcaacaucgu gcgcgaggac     3060

ggcgaggccg aguccacgag caagaaccug aucuccugca ccgggggcgu caccgcccgg     3120

cugaagaugg acuggggccu caacgacgug uggaacaacc ugaucuaccc ccgcuucgag     3180

cgccugaacg agcucaccaa cagcaaccag uucggguacu gggacaagaa ggacggcaag     3240

aacguguucc agacccaggu cccgauggac auccagaagg gguucaacaa gaagcggauc     3300

gaccaccgcc accacgcgcu ggacgcccug gugaucgccu gcgccacgcg gucccacauc     3360

aacuaccuca acaacgagag cgccaagucc aacagcaagg agucccgcua cgaccugcgg     3420

cgcaagcugc ggcgccucga ggugauccag aaggaguaca ccaucaacgg ccggaaggag     3480

auccgcaaca ucgaggucgc gaaggaguuc uacaagcccu ggaacagcuu cacccaggac     3540

gcccaguccg cccuggagaa caucguggug agcuucaagc agaaccugcg ggucaucaac     3600

aagaccacga acuacuuccu ccgcuucaac aaggagggga agaagaucuu cgagacccag     3660

accuccggca gcaacugggc cauccggaag ccccugcaca aggacaccgc cuucggccug     3720

gugaaccuca agcgcacgaa gaccgugucc cuggcgaacg ccauccugga cuacgaccag     3780

aucgucaacc ggaagcucug gaccaagauc aaggagcuga ucagcaucgg guacgacaac     3840

aagcgcaucc ugaaguacuu caccgacgac aaggagaacu ggaacgagau caucuccagc     3900

ggcaucgagg uguucuacua cacggacaac uucgagaagc aggucgccac ccggaagccc     3960

cucgacacca gcuucaccga guccaagauc aucaacagca ucacggaccu ggggauccac     4020

aagauccugc ucgcccaccu ccgcgcgaac gagggcaacc cgaacaucgc cuucuccccc     4080

gaggggaucg agaccaugaa ccggcacauc ugcgagcuga acggcggcaa guuccaccac     4140

cccaucuaca aggugcgcgu gcacgagccc cugggguaca aguucaaggu gggcgacaag     4200

gggaaccgca gcaccaagua cgucgaggcc gccaagggca ccaaccuguu cuucgggauc     4260

uacaucaacu cccagggcau ccggagcuac gagacgaucc cgcugaacgu ggccaucgag     4320

cgccagaagc agggccucuc ccccgugccc gacaacaacg agcuggggga gcggcugcug     4380

uucuggcuga gccccaacga ccuggucuac cucccgaccu ccgacgaggu ggagagcggc     4440

aucugcugca acaagcuggg gaucgaccgc aucuacaaga ugguguccug caccggcuac     4500

gucggggacu ucauccccgc gcacaucgcc aaccccauca uccagacccu ggagcucggc     4560

agcaacaaca aggcccagcg ggccuggacg ggcgagauga ucaaggagau cugcaucccc     4620

gugaaggugg accgcauggg gaacaucguc gagaucuuca accggaacaa gaacuga        4677


<210>  2295
<211>  4695
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2295
auggccaaga uccugggccu cgaccugggg accaacagca ucggcugggc caucguggac       60

gacaagaacu ccaagauccu ggacaccggg guccgcaucu uccccgaggg cgugguggcg      120

aagacgaucg ggagcggcga caaggaggag uccaagaacg ccaagcggcg cgagagccgg      180

cagacccgcc ggggcuucua ccgcaagcgg cuccgcaaga ucaagcugcu gcggacccuc      240

aucaacuuca agaugugccc gcuggagcac uucgagcugg acgucugguc caagugggac      300

aagaucaaga agaaggaggg gcgcauggcc cccgagcugu uccugcccga gaacagcgcc      360

uacaaccugu ggcucaagca gaaccccuac auccugcggg acaaggcccu guccaaggac      420

cucacccuga cggaguucgg ccgcgugcug uaccaccuca uccagcggcg cggguuccug      480

agcaaccgga agggcaagga cgacgggaag aucuacggcg gcaaggaggg gaucgugggc      540

aucgacaaga ccaacgagca gaucaagaac uccacccugg ggaaguuccu cuucaagauc      600

cugccgaagg agggcgagcc cuucaagauc aucgaggacg agcugggcaa cgagcuccgc      660

guccggagcc gcuacacccu gcgggacaug uacaucgcgg aguucgaggc caucuggaac      720

cgccaggcca agcaccuggg gcucgacaac aucuacuacu ccaacgugaa gaacgugcug      780

cugaagggca gccucaagaa caagaagaac gagcggaaga ucaaccaccu gaagaagauc      840

aaggggaacg gcaacaucga gaucaucacg cugaacaaca accucuccaa gaacgagaag      900

cccaaguacc ugcugaagau cgccaccaag cucccccuga aggaguuccu ggcggggcgc      960

aucgagaagg acgaggacgg caagaucaag uucaagagca acgaguccgu ccucuucugg     1020

cagcgcccgc ugcggagcca gaagaagcug cucgccaagu gccgcuucga gcccgaccug     1080

aaggacgaca acggcaaguu cauccagaag gggaagaccc ccugccaccu gucccacccc     1140

cucuacgagg aguaccgggc cuaccaguac gugaacaaca ucgaguacgg caagaagcag     1200

cgccuggagg agagccagcg gaagcagguc aucgaccuga ucaacuccaa ggacgggaac     1260

uucaaguuca gcgagaucaa gaagaagcuc aagcugagcu acgagaccuu caacuacucc     1320

gacgacagcu ccgugaacgg caacuacaug aucaagaacc ugaacagcau cuucccgaag     1380

gagauccugg agaaggaguu cguggugaac gucaacaagg aggacaucau cgaguacggg     1440

uacgagcgca ucuggcaccu cuuccacuuc ugcgaggaca acgacgugcu gaucaagaag     1500

cugcaggaca acuucgaccu cuccgagaac gacaucgaga agaucagcaa gaucaaccug     1560

aaggagggcu acuccaacgu gagccugaag gccaucaaga acauccuccc cuaccugaag     1620

gagggcaagc ugcucuccga cgccgucauc cucgggggcg ugcggaacgu guucgggaag     1680

aaguggcacg aguucaucga cuuccacgag aagaucgaga aggacauccu guacaucaac     1740

caccagaagu ccaacaagga gggcgaggcg aucgagaaga ucaagaagua ccucaucgac     1800

uacaaguucg gguucacgga gaacgacaac cgcuucaaga agcuguacca ccacagccag     1860

gacgucgugg aggugaagcu gcagaagaag aucggccccg ucgaggaccu ccggaacccc     1920

aucgugcagc agggcgucaa cgaggugcgc cggcugguga acgagcugcu cgacguccac     1980

gggaaguucg accaggugcg cguggagcug ggccgggacc ugaagaacaa caagagcaag     2040

cgcgaggaga ucggguacaa gauccgggag aacaccgcca agaacgagaa ggccaaggag     2100

cuccugaucg aguacggccu gcgccagucc cgggacaacg uccagaaggu gcuccuguuc     2160

caggagaugc uggaccgcgg caccguggcc gucugcccgu acaccaacaa gacgaucaac     2220

aucagcgacg ugcucgggaa cgagaacaag augcagaucg agcacaucau ccccaagucc     2280

aucagccugg acgacuccuu cgccaacaag acccugugcg acagcaaguu caacggccuc     2340

aagggggagc ggacccccua cgaguucuac cagaucaaca acgacgcgaa ccuguggggc     2400

ggguccaaga gcugggagga gaucgagcgc cgcaccuucc ggauccugcc cuacagcaag     2460

gccaagcgcu ucacguccaa gaccaaguuc gagaccaacg acuucaucca gcggcagcuc     2520

aacgacaccc gcuacaucag caagaagacg aaggagaucc ugucccagau cugcgaggac     2580

gugcggguca ugccgggcag ccugaccgcc gagcuccgca agcugugggg ccugaacaac     2640

auccuccacc ccgccuucgu gcuggaccug cccgcccucc acaucgacgc gaacaagcac     2700

aucccccacu acgugguccu ggacgaggac aacgugccga uccuguccca gcggaucuac     2760

aacgacaagc ccauccucaa ggccaaccag accagcauca ccggguucgu ggacaacgag     2820

aucuucaagu ccaaggagaa guacgucaag uucgagguga aggcccccaa ccugagcaac     2880

ggcgaguacu gggccaagcu gcuccugucc aaccccaaga gcaucgugcg cgucuucaag     2940

gagaagccga aguccacgga gaacgagauc guggugcggg ggaagaucga gaaggagaag     3000

uucaagcacg agggccugag cgggaucaac gccgagggca aggagaacgg caccuacugg     3060

gcgaagcucu ccaucgucga caagaaguuc aagaagccca agaaggacga ccagcccaag     3120

aagaacggga agcagauccu gcuguucggc gccgugaagg aggggguguu cagcuccuac     3180

auguacgagu gcgacgccga ccucgaggac ggcaaguacu ggcugauccu ggacaucgac     3240

uucgagaacg ucgaguucga gaaggcccuc aacgagcgcc cggaggugcu ggagaaccag     3300

auccugaucc aggggaucac caacgacgag aacguguucg ucagcgacau ggacugccag     3360

caccaguucc ucaugaacga gggcaagggc aaguacuacg ugcuguucga caucaucucc     3420

gaggugaacg aguucuaccc caucaagacc aagagccccg agcuggagaa cgggcagagc     3480

cucacggagg gcaccaucug ggucgacaag uacaccgggg agaucaaguu cgaccccaag     3540

aagaaccggg aggaccaccg ccaccacgcg aucgacgccc uggugaucgc ccugaccaag     3600

gagacguacu uccagcagcu cucccggcgc aacgccagcu ccgagaacaa gaagcggggc     3660

aacgaguacg agaaggagca gcuggacuuc gccgagccgu ggaaggaguu ccacguggac     3720

gcgcagaagu ccaucaacag cauccugauc agccacaagc agaacaagaa cauccucacc     3780

aagaucacca agaagaucac caagaacggg aagaccuaca aguccgaggg caugagcguc     3840

cgcggccagc ugcacgacaa gacguacuac gggcacaaca agaacugcaa ggccgacgag     3900

uacguguacc ggguggacau caacaaccug uccuucagcc agcucaccaa caucaucgac     3960

cugaaccuga aggccgucau cguggagggc gugaucaaga aguucgagga guccgagaag     4020

cuccuguuca agaagaugaa cagcaagguc cagaaccugg acaaguccaa ggugaagaag     4080

aucaaggcca agguggacaa gauccucaag gagaccagcu ucuucaugcc caacgagggg     4140

aagcgcuaca uccggcugaa caucaucgag cccgccaacu ucgagcgcau gcccgucccg     4200

aucaagaagg ugcggauccg caaggugcug cgcaacgcgg agaagcucaa caacgagauc     4260

aagcaguucg ucgaccccca gaacaaccac cacgugcuga ucuacaagga cgagaacggc     4320

aaccugaagg aggagguggu caccuucugg acgguggucg agcggaagcg caccggcgag     4380

cccguguaca agcuccccuc cgacgggaag aagaucguga ccacccugca gaucaacgac     4440

auguuccugc ucggccugaa cgaggacgag aucaacuggg agaacccgaa cuacaaccac     4500

cugaaggagc accucuaccg gguccagaag uucacgagcg gggacuucua cuuccgcauc     4560

gccaagaccu ccagcaucca gaacggcgag gagaagcagc agaucaacuc cuucgggcug     4620

ggcaagaacg gcuggcugac ccacaacccc aucaagguga agaucagcgu gagcgggaag     4680

auccucaagg ucuga                                                      4695


<210>  2296
<211>  4713
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2296
auggagaaca gcaacaacuc cagcaagaag uacuaccugg gccucgacau ggggaccgac       60

uccugcggcu ggugcgugac cgacgacaag uacaacauca uccgcagcca caagaagauc      120

uacgggcccg acggcaagcu ggucaagcgg gaggggugcc accugugggg cucccgccuc      180

uuccggccgg ccaacacggc caagagccgc cggcugaacc gcaccgcgcg gcgccggcug      240

gcccgccggc gccggcgcau cauccuccug cgggagcugu uccagcccga gaucgacaag      300

guggacccca acuucuuccg ccggcucgac caguccaacc uguggaacga ggacaaggac      360

aaggagcugc agauggagca caacuuccuc uucaacgaca aggaccugac cgacaaggag      420

uacuacaagg acuuccccac caucuaccac cuccgcaagg ccauggugaa cggccugacg      480

aucaacgggu acuacuacga cccgaagaag caguucgaca uccgggaggu cuaccuggug      540

cuggcccaca ugaucaagua ccgcggcaac uuccucaugg agggggacgu gaaggacagc      600

gucguggacc ccaccuaccu gaagcagugc uucgacaaca ucgacgugau ccugaucucc      660

cucaacaacg gcgaggacga cgagaacgag gacgacaaga ucgaccccag cgagugcugc      720

uucaacgucg acgagaagaa ggcccggaag cuggcgaaca acuuccagga gaccaacaag      780

guguccgacc uggaggacga cgagcgcgac cucuucggga aguucaacag caaggacauc      840

cgcaagaaga uccugaagcu gaucaacggc ggcuccgggg ugaagcucaa ggacuuccug      900

cccaaggaga uccuggucgc ccacgacggc gacgacgagu acggggacaa gaagaucgac      960

cucagcuccg agacccugga ggacgacauc aacggcuaca gccugccgga guccuacgug     1020

gagaucaucc ucgaggccaa gaagaucuac gacuucaaga cgcuggccua ccugcuccac     1080

ggcaagagcc ugcugugcga cgcgauggug aacgucuacg aggagcacaa ggagcagcuc     1140

gccgcccuga agcggcugua caagaaguac gccaccaagg agcaguacua cuucauguuc     1200

cgcgacuccc ggauccucga cggggacgac agcgacgacg aggaggacaa ggaggagagc     1260

aacaaggagg aguccaagga caagaagaag aaggccagcu acguguccua caucggcuac     1320

accaagacca acagcaaggc gaaguacacg ggggcccaca ucaccuccga gaagaacauc     1380

agcgcccuga ucaagcagau caaggacgac cugaaguucg acgaccucaa guccaaggug     1440

gacgacccca gccagcagaa cgagcugucc gacgaggaca agcuggacau ccagugcauc     1500

gaggaggcca ucgagagcaa caccuuccuc ccccgccaga acucccggaa caacggcgcc     1560

uuccccuacc agcugaaccu gaacgagaug cgcaccauca ucgagaacca gagcaaguac     1620

uacccguucc ugaaggacaa ggcgcccucc uuccccgacg ggaagacgga ggaguacaag     1680

aucgucagcc uccugaaguu caagaucccc uacuacaucg gcccgcuguc ccagcccaac     1740

gacuacgugc gggacgacaa gcgcgacaac cacugggugc aguuccgggg cgagaacaac     1800

caggggauca ucaaccccug gaacuucagc cagaucgucg accucgaggg caccgccgac     1860

aaguucaucu ccaaccugac caacuacugc accuacaucu ucggggagaa cacgcugccc     1920

aaggagagcc ucaucuucca ggccuacaag gugcugaacg accugaacaa ccucaaggug     1980

aacggcuccc ugaucagcaa ggaggacaag gacuacauca uccagaacgu cuaccugaag     2040

aagaagaaga ucaaggggaa ggacaucgug aacgcccuca agaacaagua caagcugagc     2100

gacgaggacc cgaccgucac caccaagggc uccggcaaga gcaucgggga ggacgaggug     2160

gaccugacga uguucaacuc cagccucucc agcuucaucg acauguccga ccccaacgcc     2220

uucggccccg aguucuacaa ggacagcaac aaguucgagc uggcggagaa ggugaucgag     2280

cugaucacca ucgucgagga caaggaccuc cugaaggagc gccugaagaa gcucgggcug     2340

uccgacaacc aggugaacua ccuguucaag cagaaguaca gcggcugggc gccccucucc     2400

cggaagcugc uggacgggau caccaucgac ugcgugaacg agaacaccgg cgaggccuac     2460

ccggucacga ucaucgacgu gaugcgcgac acgccccuca acuucaugga gaucuacgag     2520

accaaggaga gccaguucuc cggcuucaag gagaaggugg ccgagcugaa cgacgagaag     2580

aagcugagcc gggacgaccu caucgaggag uccuacgcca gccccgccau gaagcgcucc     2640

guccggcaga ccuucaaggu gaucgacgag cugaagaaca cccugaacau cgaccacuuc     2700

gaccgcaucu ucguggaggc cacccgggcc cccggggagg ccggcaagau ggucaagagc     2760

cgcaagaagc agcuccagga ggcguacgac gccgccaagg accuggugga ccaggagagc     2820

cgggacgacc ugcagaagcu ccgcgacgac cuggagaagc agaccgacga ccagcugcgc     2880

cggaagaagg uguaccucua cuucaugcag cuggggcgcu ccgucuacag cggcgagaag     2940

aucgaccuga acgagcucga caagaacuac gacaucgacc acaucauccc gcaggccaag     3000

cugaaggacg acuccuucaa caacauggug cugguggagc gggagcucaa caacaagaag     3060

caggacgagu accccauccc cagcaccauc aucuccgagc acggccgcaa cuggauccag     3120

aagcuggcca agauccagga caagaacaag cggagcuacu ucaugccgaa ggacaaggcg     3180

aaccgccugc uccggcccga guccaagcag cugacgagcg acgagcugaa cggguucauc     3240

aaccgccagc ucacccugac caaccagucc gucaaggccg ugugcgacau ccugaagaug     3300

accgagaagg gcagcgagau cguguacucc aaggccaacu ucgucagcga cuuccggaag     3360

cucugcgggg agcugcgccc cuccuacgac gccuucguga aggugcggga caucaacgac     3420

uuccaccacg ccaacgacgc guaccugaac aucgucgugg gcaacgugua cuacgagaag     3480

uucaagaacu ucuacaucaa caaccgcauc gacuacgaca acaacgggaa gaaggaccuc     3540

agcguccugg ccuccaagcu guucaccaag acgcaggccg acgacaagac cggcaacauc     3600

uacugggagg cccgguucca caacgagaag gagaccgaca gcaacggcaa ggagcgcacc     3660

aucaaggugc gggacgaggg gacggagucc accuucgaca agaucgagua cuacauggag     3720

cucaacgacc cccuggugac ccagaugcag uacacccuga gcggcaagca gggguucuuc     3780

aagaagauca cgcucaagac caaggccaag gacaacgagg acaacuccga caagcuguuc     3840

ccgcugaagc aguaccuccg cagcgacagc aacaagguca uggacgugaa caaguacggc     3900

ggguacuccg acaugaucac ccccuacuuc augcuggugc agagcgaggg caagaagggc     3960

aagcacaucu acucccugga gagcaucccc accaucuacc ucaaguccuu caagaagaag     4020

gaggaggaca ucaucaacua ccugagcugc aagaacaagc ugaagaaccc cgucgugauc     4080

uccgacaagc uccugaugcg gacgaucgug aagaucccgu acuucaagaa cgacagcaac     4140

cugucccucg ucaagcuggg gaccagcggc cgcaccggga acaacaucau cuacaucaac     4200

cuguccgagc ucauccugcc caucgagugg aagaacuacu accggaagau cagcaaccug     4260

cucggcacca acguguccag cacgaagccc aaggaccugu ccaaguucac cgagcagaug     4320

aagaacgacc ccaccagcac ccugguggac ggguccaaca aggucgagca cgacaagaac     4380

aaccgcuucu ucagcuaccu caucaacaac gugaucaaca agccgaucuu cgagggccug     4440

cccaagacgu ccugcagcuu caucaagcug gagagcaagu acgacgaguu cugcucccuc     4500

aacacccugg agcaggcgaa gaucaucgcc accaucauca agcugcucag cugcaagucc     4560

gugcagggcu gcaagcugag caaccugucc gggcucccca gcucccuggg cgcccugauc     4620

agcaacaagg cccugaccga ggggacgauc aucuccaagg agagcuccug cggccucaag     4680

gagaacauca ucuucgucgu gcccaacgac uga                                  4713


<210>  2297
<211>  5067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2297
auggaguuca agaaggugcu gggccucgac aucgggacca acagcaucgg cugcgcccug       60

cugucccucc ccaagagcau ccaggacuac gggaagggcg ggcgccugga guggcugacc      120

ucccggguca ucccgcucga cgccgacuac augaaggcgu ucaucgacgg caagaacggc      180

cugccccagg ugaucacgcc cgccgggaag cgccggcaga agcgcggcag ccggcgccug      240

aagcaccggu acaagcuccg ccggucccgc cugauccggg uguucaagac ccugaacugg      300

cuccccgagg acuucccgcu ggacaacccc aagcgcauca aggagaccau cagcaccgag      360

gggaaguucu ccuuccggau cagcgacuac guccccaucu ccgacgagag cuaccgcgag      420

uucuaccggg aguucggcua ccccgagaac gagaucgagc aggugaucga ggagaucaac      480

uuccgccgga agacgaaggg gaagaacaag aacccgauga ucaagcugcu ccccgaggac      540

uggguggucu acuaccugcg caagaaggcc cugaucaagc ccaccaccaa ggaggagcuc      600

auccggauca ucuaccuguu caaccagcgc cggggcuuca aguccagccg caaggaccug      660

accgagacgg ccauccucga cuacgacgag uucgccaagc gccuggcgga gaaggagaag      720

uacuccgccg agaacuacga gaccaaguuc gugagcauca ccaaggugaa ggaggucgug      780

gagcugaaga ccgacggccg gaaggggaag aagcgcuuca aggugauccu cgaggacucc      840

cggaucgagc ccuacgagau cgagcgcaag gagaagccgg acugggaggg caaggaguac      900

acguuccugg ucacccagaa gcuggagaag gggaaguuca agcagaacaa gcccgaccuc      960

cccaaggagg aggacugggc ccugugcacc accgcccugg acaaccggau gggcagcaag     1020

caccccggcg aguucuucuu cgacgagcuc cugaaggcgu ucaaggagaa gcgcggguac     1080

aagauccggc aguacccggu gaaccgcugg cgguacaaga aggagcugga guucaucugg     1140

acgaagcagu gccagcucaa ccccgagcug aacaaccuga acaucaacaa ggagauccuc     1200

cgcaagcugg ccaccgugcu guaccccucc cagagcaagu ucuucggccc caagaucaag     1260

gaguucgaga acuccgacgu ccuccacauc aucagcgagg acaucaucua cuaccagcgg     1320

gaccugaaga gccagaaguc ccugaucagc gagugccgcu acgagaagcg gaaggggauc     1380

gacggcgaga ucuacgggcu caagugcauc ccgaagucca gcccccugua ccaggaguuc     1440

cgcaucuggc aggacaucca caacaucaag gugauccgga aggaguccga ggugaacggc     1500

aagaagaaga ucaacaucga cgagacccag cuguacauca acgagaacau caaggagaag     1560

cucuucgagc uguucaacag caaggacucc cugagcgaga aggacauccu cgagcugauc     1620

ucccugaaca ucaucaacag cggcaucaag aucuccaaga aggaggagga gaccacgcac     1680

cgcaucaacc uguucgccaa ccggaaggag cucaagggga acgagaccaa gagccgcuac     1740

cggaaggucu ucaagaagcu gggcuucgac ggggaguaca uccugaacca ccccuccaag     1800

cucaaccgcc uguggcacag cgacuacucc aacgacuacg ccgacaagga gaagaccgag     1860

aagagcaucc uguccagccu cggcuggaag aaccgcaacg ggaaguggga gaaguccaag     1920

aacuacgacg uguucaaccu gccccuggag guggccaagg cgaucgccaa ccucccgccc     1980

cugaagaagg aguacggcag cuacagcgcc cuggccaucc ggaagaugcu cgucgugaug     2040

cgcgacggca aguacuggca gcaccccgac cagaucgcca aggaccagga gaacaccucc     2100

cugaugcugu ucgacaagaa ccucauccag cugacgaaca accagcggaa ggugcugaac     2160

aaguaccucc ugacccuggc ggagguccag aagcgcagca cccucaucaa gcagaagcug     2220

aacgagaucg agcacaaccc cuacaagcug gagcucgugu ccgaccagga ccuggagaag     2280

cagguccuga agagcuuccu cgagaagaag aacgaguccg acuaccugaa ggggcugaag     2340

accuaccagg ccggcuaccu caucuacggg aagcacagcg agaaggacgu gccgaucgug     2400

aacucccccg acgagcuggg cgaguacauc cggaagaagc ugcccaacaa cagccuccgc     2460

aacccgaucg ucgagcaggu gauccgggag acgaucuuca ucgugcgcga cgucuggaag     2520

uccuucggga ucaucgacga gauccacauc gagcugggcc gggagcugaa gaacaacagc     2580

gaggagcgca agaagaccuc cgagagccag gagaagaacu uccaggagaa ggagcgggcc     2640

cgcaagcucc ugaaggagcu gcucaacucc agcaacuucg agcacuacga cgagaacggc     2700

aacaagaucu ucuccagcuu caccgugaac cccaaccccg acuccccccu ggacaucgag     2760

aaguuccgga ucuggaagaa ccagagcggg cugaccgacg aggagcucaa caagaagcug     2820

aaggacgaga agaucccgac ggagaucgag gugaagaagu acauccugug gcucacccag     2880

aagugccgca gccccuacac cggcaagauc aucccccugu ccaagcuguu cgacagcaac     2940

gucuacgaga ucgagcacau caucccccgg uccaagauga agaacgacag caccaacaac     3000

cucgugaucu gcgagcuggg ggugaacaag gccaagggcg accgccuggc cgcgaacuuc     3060

aucuccgaga gcaacggcaa gugcaaguuc ggggaggucg aguacacgcu ccugaaguac     3120

ggcgacuacc ugcaguacug caaggacacc uucaaguacc agaaggccaa guacaagaac     3180

cuccuggcca ccgagccgcc cgaggacuuc aucgagcggc agaucaacga cacccgcuac     3240

aucgggcgga agcuggccga gcuccugacc cccgugguga aggacuccaa gaacaucauc     3300

uucacgaucg gcagcaucac cuccgagcug aagaucaccu gggggcucaa cggcgucugg     3360

aaggacaucc ugcgcccccg guucaagcgc cuggagagca ucaucaacaa gaagcucauc     3420

uuccaggacg aggacgaccc gaacaaguac cacuucgacc uguccaucaa cccccagcug     3480

gacaaggagg gccucaagcg ccuggaccac cggcaccacg cccuggacgc gaccaucauc     3540

gccgccacga cccgcgagca cgugcgguac cucaacagcc ugaacgccgc ggacaacgac     3600

gaggagaagc gcgaguacuu ccugucccuc ugcaaccaca agauccggga cuucaagcug     3660

cccugggaga acuucaccag cgaggugaag uccaagcugc ucagcugcgu cguguccuac     3720

aaggagagca agcccauccu guccgacccg uucaacaagu accugaagug ggaguacaag     3780

aacgggaagu ggcagaaggu guucgccauc cagaucaaga acgaccgcug gaaggccguc     3840

cggcgcagca uguucaagga gcccaucggc accgugugga ucaagaagau caaggaggug     3900

agccucaagg aggccaucaa gauccaggcc aucugggagg aggucaagaa cgaccccgug     3960

cggaagaaga aggagaagua caucuacgac gacuacgcgc agaaggugau cgccaagauc     4020

guccaggagc uggggcuguc cagcuccaug cgcaagcagg acgacgagaa gcucaacaag     4080

uucaucaacg aggccaaggu gagcgccggc gugaacaaga accugaacac gaccaacaag     4140

accaucuaca accuggaggg gcgguucuac gagaagauca aggucgccga guacgugcuc     4200

uacaaggcga agcgcaugcc ccugaacaag aaggaguaca ucgagaagcu gucccuccag     4260

aagauguuca acgaccugcc gaacuucauc cuggagaaga gcauccucga caacuacccc     4320

gagauccuga aggagcugga guccgacaac aaguacauca ucgagcccca caagaagaac     4380

aaccccguga accggcuccu gcuggagcac auccuggagu accacaacaa cccgaaggag     4440

gccuucagca ccgagggccu cgagaagcug aacaagaagg ccaucaacaa gaucggcaag     4500

cccaucaagu acaucacgcg ccuggacggg gacaucaacg aggaggagau cuuccggggc     4560

gccgucuucg agaccgacaa gggguccaac guguacuucg ugauguacga gaacaaccag     4620

accaaggacc gcgaguuccu caagcccaac ccgagcaucu ccguccugaa ggccaucgag     4680

cacaagaaca agaucgacuu cuucgcgccc aaccggcugg gcuucagccg caucauccuc     4740

ucccccgggg accuggugua cgugcccacc aacgaccagu acguccugau caaggacaac     4800

agcuccaacg agacgaucau caacugggac gacaacgagu ucaucagcaa ccggaucuac     4860

caggugaaga aguucaccgg caacuccugc uacuuccuca agaacgacau cgccagccug     4920

auccuguccu acagcgccag caacggcgug ggggaguucg gcucccagaa caucagcgag     4980

uacuccgucg acgacccgcc cauccgcauc aaggacgugu gcaucaagau ccgcguggac     5040

cggcucggga acguccgccc ccuguga                                         5067


<210>  2298
<211>  3924
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2298
augacccagu ucgagggcuu caccaaccug uaccagguga gcaagacgcu ccgcuucgag       60

cugauccccc aggggaagac ccugaagcac auccaggagc agggcuucau cgaggaggac      120

aaggcccgga acgaccacua caaggagcuc aagccgauca ucgaccgcau cuacaagacc      180

uacgccgacc agugccugca gcugguccag cucgacuggg agaaccuguc cgcggccauc      240

gacagcuacc ggaaggagaa gaccgaggag acgcgcaacg cccugaucga ggagcaggcc      300

accuaccgga acgccaucca cgacuacuuc aucgggcgca ccgacaaccu caccgacgcg      360

aucaacaagc ggcacgccga gaucuacaag ggccuguuca aggccgagcu guucaacggg      420

aaggugcuca agcagcuggg cacggugacc accaccgagc acgagaacgc ccugcuccgc      480

uccuucgaca aguucacgac cuacuucagc ggcuucuacg agaaccggaa gaacgucuuc      540

uccgcggagg acaucagcac cgccaucccc caccgcaucg ugcaggacaa cuuccccaag      600

uucaaggaga acugccacau cuucacccgg cugaucacgg ccgugcccuc ccugcgcgag      660

cacuucgaga acgucaagaa ggccaucggg aucuucguga gcaccuccau cgaggaggug      720

uucagcuucc cguucuacaa ccagcuccug acccagaccc agaucgaccu guacaaccag      780

cuccugggcg ggaucucccg ggaggccggc acggagaaga ucaaggggcu gaacgagguc      840

cucaaccugg cgauccagaa gaacgacgag accgcccaca ucaucgccag ccugccccac      900

cgcuucaucc cccucuucaa gcagauccug uccgaccgga acacccugag cuucauccuc      960

gaggaguuca aguccgacga ggaggugauc cagagcuucu gcaaguacaa gacccugcug     1020

cgcaacgaga acgugcucga gacggccgag gcccuguuca acgagcugaa cuccaucgac     1080

cucacccaca ucuucaucag ccacaagaag cuggagacca ucuccagcgc gcugugcgac     1140

cacugggaca cccuccggaa cgcccuguac gagcgccgga ucagcgagcu gacgggcaag     1200

aucaccaagu ccgccaagga gaagguccag cgcagccuca agcacgagga caucaaccug     1260

caggagauca ucuccgccgc cggcaaggag cugagcgagg cguucaagca gaagaccucc     1320

gagauccuca gccacgccca cgccgcccug gaccagcccc ugccgaccac gcucaagaag     1380

caggaggaga aggagauccu gaagucccag cuggacagcc ugcucgggcu guaccaccuc     1440

cucgacuggu ucgccgugga cgaguccaac gagguggacc ccgaguucag cgcgcggcug     1500

accggcauca agcuggagau ggagcccucc cucagcuucu acaacaaggc ccgcaacuac     1560

gccaccaaga agcccuacuc cgucgagaag uucaagcuga acuuccagau gccgacccug     1620

gccagcgggu gggacgugaa caaggagaag aacaacggcg cgauccucuu cgugaagaac     1680

ggccuguacu accuggggau caugcccaag cagaagggcc gcuacaaggc ccucuccuuc     1740

gagcccacgg agaagaccag cgagggguuc gacaagaugu acuacgacua cuuccccgac     1800

gccgccaaga ugaucccgaa gugcuccacc cagcugaagg ccgucaccgc gcacuuccag     1860

acccacacga cccccauccu gcucagcaac aacuucaucg agccccugga gaucaccaag     1920

gagaucuacg accugaacaa ccccgagaag gagccgaaga aguuccagac cgccuacgcc     1980

aagaagacgg gcgaccagaa gggguaccgg gaggcccucu gcaaguggau cgacuucacc     2040

cgcgacuucc uguccaagua caccaagacc acgagcaucg accugagcuc ccuccggccc     2100

agcucccagu acaaggaccu gggcgaguac uacgccgagc ugaacccccu ccuguaccac     2160

aucagcuucc agcgcaucgc ggagaaggag aucauggacg ccguggagac cggcaagcug     2220

uaccucuucc agaucuacaa caaggacuuc gccaaggggc accacggcaa gcccaaccug     2280

cacacccugu acuggaccgg ccucuucucc ccggagaacc uggccaagac gagcaucaag     2340

cugaacgggc aggccgagcu guucuaccgg cccaaguccc gcaugaagcg gauggcgcac     2400

cgccuggggg agaagaugcu gaacaagaag cucaaggacc agaagacccc cauccccgac     2460

acccuguacc aggagcugua cgacuacgug aaccaccggc ucagccacga ccuguccgac     2520

gaggcccgcg cccugcuccc gaacgucauc accaaggagg ugagccacga gaucaucaag     2580

gaccggcgcu ucacguccga caaguucuuc uuccacgugc ccaucacccu gaacuaccag     2640

gccgcgaaca gccccuccaa guucaaccag cgggucaacg ccuaccugaa ggagcacccg     2700

gagaccccca ucaucggcau cgaccgcggc gagcggaacc ucaucuacau caccgugauc     2760

gacagcacgg ggaagauccu ggagcagcgc ucccugaaca ccauccagca guucgacuac     2820

cagaagaagc ucgacaaccg ggagaaggag cgcgucgccg cccggcaggc cuggagcgug     2880

gugggcacca ucaaggaccu gaagcagggg uaccuguccc aggucaucca cgagaucgug     2940

gaccucauga uccacuacca ggcggugguc gugcuggaga accugaacuu cggcuucaag     3000

agcaagcgca ccgggaucgc cgagaaggcc guguaccagc aguucgagaa gaugcucauc     3060

gacaagcuga acugccuggu ccucaaggac uaccccgccg agaagguggg cggcgugcug     3120

aaccccuacc agcugacgga ccaguucacc agcuucgcca agauggggac ccaguccggc     3180

uuccucuucu acgucccggc gcccuacacc agcaagaucg acccccugac gggguucgug     3240

gaccccuucg uguggaagac caucaagaac cacgaguccc gcaagcacuu ccuggagggc     3300

uucgacuucc uccacuacga cgucaagacc ggcgacuuca uccugcacuu caagaugaac     3360

cggaaccuga gcuuccagcg cgggcucccg ggcuucaugc ccgccuggga caucguguuc     3420

gagaagaacg agacccaguu cgacgccaag gggacgcccu ucaucgccgg caagcggauc     3480

gugcccguca ucgagaacca ccgcuucacc gggcgguacc gcgaccugua cccggccaac     3540

gagcugaucg cgcuccugga ggagaagggc aucguguucc gggacggcuc caacauccug     3600

cccaagcucc uggagaacga cgacagccac gccaucgaca ccaugguggc ccugauccgc     3660

uccguccucc agaugcggaa cagcaacgcc gcgaccgggg aggacuacau caacuccccc     3720

gugcgcgacc ugaacggcgu gugcuucgac agccgguucc agaaccccga guggccgaug     3780

gacgccgacg ccaacggggc cuaccacauc gcccugaagg gccagcuccu gcugaaccac     3840

cucaaggagu ccaaggaccu gaagcugcag aacgggauca gcaaccagga cuggcucgcg     3900

uacauccagg agcugcgcaa cuga                                            3924


<210>  2299
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2299
augagcaucu accaggaguu cgugaacaag uacucccuga gcaagacccu ccgcuucgag       60

cugauccccc agggcaagac ccuggagaac aucaaggccc gggggcucau ccuggacgac      120

gagaagcgcg ccaaggacua caagaaggcg aagcagauca ucgacaagua ccaccaguuc      180

uucaucgagg agauccuguc cagcgucugc aucuccgagg accuccugca gaacuacagc      240

gacguguacu ucaagcugaa gaaguccgac gacgacaacc uccagaagga cuucaagagc      300

gccaaggaca cgaucaagaa gcagaucucc gaguacauca aggacagcga gaaguucaag      360

aaccuguuca accagaaccu gaucgacgcc aagaagggcc aggaguccga ccucauccug      420

uggcugaagc agagcaagga caacgggauc gagcuguuca aggccaacuc cgacaucacc      480

gacaucgacg aggcccucga gaucaucaag agcuucaagg gcuggaccac cuacuucaag      540

ggguuccacg agaaccggaa gaacguguac uccagcaacg acaucccgac guccaucauc      600

uaccgcaucg ucgacgacaa ccugcccaag uuccucgaga acaaggcgaa guacgagagc      660

cugaaggaca aggcccccga ggccaucaac uacgagcaga ucaagaagga ccuggccgag      720

gagcucaccu ucgacaucga cuacaagacc uccgagguga accagcgggu guucagccug      780

gacgaggucu ucgagaucgc gaacuucaac aacuaccuga accagagcgg caucaccaag      840

uucaacacga ucaucggcgg gaaguucgug aacggcgaga acaccaagcg caaggggauc      900

aacgaguaca ucaaccucua cucccagcag aucaacgaca agacccugaa gaaguacaag      960

augagcgugc uguucaagca gauccucucc gacaccgaga gcaaguccuu cgucaucgac     1020

aagcuggagg acgacagcga cguggugacg accaugcagu ccuucuacga gcagaucgcc     1080

gccuucaaga ccgucgagga gaaguccauc aaggagaccc ugagccuccu guucgacgac     1140

cugaaggccc agaagcucga ccugagcaag aucuacuuca agaacgacaa gucccugacg     1200

gaccucagcc agcaggucuu cgacgacuac uccgugaucg gcaccgccgu gcuggaguac     1260

aucacccagc agaucgcgcc caagaaccug gacaacccga gcaagaagga gcaggagcuc     1320

aucgccaaga agaccgagaa ggccaaguac cugucccugg agacgaucaa gcucgcccug     1380

gaggaguuca acaagcaccg ggacaucgac aagcagugcc gcuucgagga gauccuggcc     1440

aacuucgcgg ccauccccau gaucuucgac gagaucgccc agaacaagga caaccucgcc     1500

cagaucagca ucaaguacca gaaccagggg aagaaggacc ugcugcaggc cuccgcggag     1560

gacgacguga aggccaucaa ggaccuccug gaccagacca acaaccugcu gcacaagcuc     1620

aagaucuucc acaucagcca guccgaggac aaggccaaca uccuggacaa ggacgagcac     1680

uucuaccugg uguucgagga gugcuacuuc gagcucgcca acaucguccc ccuguacaac     1740

aagauccgga acuacaucac ccagaagccc uacagcgacg agaaguucaa gcugaacuuc     1800

gagaacagca cccucgccaa cggcugggac aagaacaagg agccggacaa cacggcgauc     1860

cuguucauca aggacgacaa guacuaccug ggcgugauga acaagaagaa caacaagauc     1920

uucgacgaca aggccaucaa ggagaacaag ggggagggcu acaagaagau cguguacaag     1980

cuccugcccg gggccaacaa gaugcugccc aaggucuucu ucuccgccaa gagcaucaag     2040

uucuacaacc ccuccgagga cauccuccgc auccggaacc acagcaccca caccaagaac     2100

ggcuccccgc agaagggcua cgagaaguuc gaguucaaca ucgaggacug ccgcaaguuc     2160

aucgacuucu acaagcagag caucuccaag caccccgagu ggaaggacuu cggguuccgg     2220

uucagcgaca cccagcgcua caacuccauc gacgaguucu accgggaggu ggagaaccag     2280

ggcuacaagc ugacguucga gaacaucagc gaguccuaca ucgacagcgu ggucaaccag     2340

gggaagcugu accucuucca gaucuacaac aaggacuucu ccgcguacag caagggccgc     2400

cccaaccugc acacccugua cuggaaggcc cucuucgacg agcggaaccu gcaggacgug     2460

gucuacaagc ugaacgggga ggccgagcug uucuaccgca agcaguccau ccccaagaag     2520

aucacccacc cggccaagga ggccaucgcg aacaagaaca aggacaaccc caagaaggag     2580

agcguguucg aguacgaccu caucaaggac aagcgguuca ccgaggacaa guucuucuuc     2640

cacugcccca ucacgaucaa cuucaagucc agcggcgcca acaaguucaa cgacgagauc     2700

aaccugcucc ugaaggagaa ggccaacgac gugcacaucc ugagcaucga ccgcggcgag     2760

cggcaccucg ccuacuacac ccuggucgac gggaagggca acaucaucaa gcaggacacc     2820

uucaacauca ucgggaacga ccgcaugaag accaacuacc acgacaagcu ggccgcgauc     2880

gagaaggacc gcgacuccgc ccggaaggac uggaagaaga ucaacaacau caaggagaug     2940

aaggagggcu accucagcca gguggugcac gagaucgcca agcuggucau cgaguacaac     3000

gccaucgugg uguucgagga ccugaacuuc ggguucaagc gcggccgguu caaggucgag     3060

aagcaggucu accagaagcu cgagaagaug cugaucgaga agcugaacua ccucguguuc     3120

aaggacaacg aguucgacaa gacgggcggg gugcugcgcg ccuaccagcu gaccgcgccc     3180

uucgagaccu ucaagaagau gggcaagcag accgggauca ucuacuacgu gccggccggc     3240

uucaccucca agaucugccc cgugacgggg uucgucaacc agcucuaccc caaguacgag     3300

agcgugucca agagccagga guucuucucc aaguucgaca agaucugcua caaccuggac     3360

aagggcuacu ucgaguucag cuucgacuac aagaacuucg gcgacaaggc cgccaagggg     3420

aaguggacca ucgcguccuu cggcagccgg cugaucaacu uccgcaacuc cgacaagaac     3480

cacaacuggg acacccggga gguguacccc accaaggagc ucgagaagcu gcugaaggac     3540

uacagcaucg aguacgggca cggcgagugc aucaaggccg ccaucugcgg cgaguccgac     3600

aagaaguucu ucgccaagcu cacgagcguc cugaacacca uccugcagau gcgcaacucc     3660

aagaccggga ccgagcucga cuaccugauc agcccggugg ccgacgugaa cggcaacuuc     3720

uucgacuccc ggcaggcgcc caagaacaug ccccaggacg ccgacgccaa cggggccuac     3780

cacaucggcc ugaaggggcu caugcugcug ggccgcauca agaacaacca ggagggcaag     3840

aagcucaacc uggucaucaa gaacgaggag uacuucgagu ucgugcagaa ccggaacaac     3900

uga                                                                   3903


<210>  2300
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2300
augaagcccg acaucaagga ccgcgaguac auguaccggc ugaucaucgg ccagcuguuc       60

uacgacgggc accagcagau cgccgugaac cucgccaaca cccugggcug cagcgcgccg      120

cccccgccgc ccuccgacaa gcuguuccgc cucgucacca ucgccaagca guucguggag      180

gaccccgacu acaaggccga ggagaacacg augcccaugc aguucgagac cagcuccgcc      240

gggcuggacc uccaguacga cgccgacgug gcggccacca gccccgagcc cucccaguac      300

gagaccaucu accugacggu ccacaaggcc cccugccggg ccgcggccuu caacagcgac      360

ggcucccuga ucgccaccgg gagcgccgac ugcuccauca agaucaugga cguggagcgc      420

auccucgccc gggagaagga gcaccgcgag augaacgaga acggccccga cgcgcaccac      480

cccgugaucc ggacccugua cgaccacguc gacgugcgcc gggacuucgg ccgcuucagc      540

gacaagaucc uggacuucca ggacgugaac accgucaucu uccacccgcg ggacgccauc      600

cucaucuccg ggagcaacga caagacggug aagcuguucg acuucuccaa gaccgccgug      660

aagcgcgcca ugaagacccu gagcgagguc uaccccgugc gggcccucuc cugccacccc      720

ggcggggagu uccugcuggu ggcgaccgac caccccacgg uccgccucua caacaucgag      780

accgcccagg ccuacgccag cgccaacccg gacgaccagc accgggaguc cgugaccgac      840

gugcacuaca gcgagaacgc gcgccuguac gucaccgccu ccaaggacgg cgacgugaag      900

gugugggacg gggucagcag ccggugcgug gagacguuca agcgcgccca cgacggcucc      960

agcaucugcu ccgccaaguu caccaagaac ggcaaguaca uccugaccag cgggauggac     1020

uccaucguga agcucuggga gcugagcacc aaccggugcc ugaucgucua cacgggcgcc     1080

ggggcgaccg gcgcccagga cuaccccacc uccgccagcu ucaaccagaa cgaggacuuc     1140

guccucuucc ccgacgagaa guccggcagc augugcuccu gggaggcccg caacgcggac     1200

cggaagcgcc ugcuggcccu cgggcacacc agcgccugcc gcacguucgu gcacuccccc     1260

agcaugccgg ccuucaugac cggcuccgac gaccaccggg cccgcuucug guaccggaag     1320

cccaccacca acgacaacug a                                               1341


<210>  2301
<211>  1293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2301
augaagcccg acaucaagga ccgcgaguac auguaccggc ugaucaucgg ccagcuguuc       60

uacgacgggc accagcagau cgccgugacc cucgccaaca cccugggcug cagcgcgccg      120

gccccgccgc ccuccgacaa gcucuaccgc cuggucacga ucgccaagca guucguggag      180

gaccccgacu acaaggccga ggagaagucc cugcccaugc aguucgagac cgugagcacc      240

gggcucgacc uggaguacga cgccgacguc cagcccguga gccccgagcc cucccaguac      300

gagaccaucu accugacggu gcacaaggcg cccugccggg ccgccgccuu caacagcgac      360

ggcucccucg ucgcgaccgg gagcgccgac ugcuccauca agaucaugga cguggagcgc      420

auccuggccc gggagaagga gcaccgcgag augaacgaga acggccccga cgcccaccac      480

cccgugaucc ggacccugua cgaccacguc gacgacguga acaccguccu cuuccacccg      540

cgcgacgcca uccugaucag cggcuccaac gacaagacgg ugaagcuguu cgacuucagc      600

aagaccgcgg ugaagcgggc caugaagacc cucuccgagg uguaccccgu ccgcgcccug      660

agcugccacc ccgggggcga guuccugcuc guggccaccg accaccccac ggugcggcug      720

uacaacaucg agaccgccca ggcguacgcc uccgccaacc cggacgacca gcaccgcgag      780

agcgucaccg acgugcacua cuccgagaac gcccggcugu acgugaccgc cagcaaggac      840

ggggacguca agaucuggga cggcgugucc aaccgcugcg uggagacguu caagcgggcg      900

cacgacggga gcgccaucug cagcgccaag uucaccaaga acggcaagua ccuccugacc      960

uccggcaugg acagcaucgu caagcugugg gagcucucca ccaaccgcug ccugaucgug     1020

uacacggggg ccggcgccac cggggcgcag gacuacgcca ccagcgccuc cuucaaccac     1080

aacgaggacu acguccuguu ccccgacgag aaguccggca gcaugugcag cugggacgcc     1140

cggaacuccg accgcaagcg gcuccuggcg cugggccaca ccagcgccug ccgcacguuc     1200

gugcacuccc ccagcaugcc cgccuucaug accggguccg acgaccaccg ggcccgcuuc     1260

ugguaccgca agccggccac caccgacaac uga                                  1293


<210>  2302
<211>  3690
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2302
auggccgcca gcaagcugga gaaguucacc aacugcuacu cccucagcaa gacccugcgc       60

uucaaggcga uccccguggg caagacgcag gagaacaucg acaacaagcg gcugcucguc      120

gaggacgaga agcgcgccga ggacuacaag ggggugaaga agcugcugga ccgguacuac      180

cucuccuuca ucaacgacgu gcugcacagc aucaagcuga agaaccucaa caacuacauc      240

ucccuguucc gcaagaagac ccggaccgag aaggagaaca aggagcugga gaaccucgag      300

aucaaccugc gcaaggagau cgccaaggcc uucaagggcg ccgcggggua caagagccug      360

uucaagaagg acaucaucga gaccauccuc ccggaggccg ccgacgacaa ggacgagauc      420

gcccugguca acuccuucaa cggcuucacg accgcguuca ccggguucuu cgacaaccgg      480

gagaacaugu ucagcgagga ggccaagucc accagcaucg ccuuccgcug caucaacgag      540

aaccugacgc gguacaucuc caacauggac aucuucgaga agguggacgc caucuucgac      600

aagcacgagg ugcaggagau caaggagaag auccucaaca gcgacuacga cgucgaggac      660

uucuucgagg gcgaguucuu caacuucgug cugacccagg agggcaucga cguguacaac      720

gccaucaucg ggggcuucgu caccgagucc ggggagaaga ucaagggccu gaacgaguac      780

aucaaccucu acaacgcgaa gaccaagcag gcccugccca aguucaagcc ccuguacaag      840

cagguccuca gcgaccgcga gucccugagc uucuacgggg agggcuacac guccgacgag      900

gaggugcugg agguguuccg gaacacccuc aacaagaaca gcgagaucuu cuccagcauc      960

aagaagcugg agaagcuguu caagaacuuc gacgaguaca gcuccgccgg caucuucgug     1020

aagaacgggc ccgccaucag caccaucucc aaggacaucu ucggcgagug gaaccucauc     1080

cgcgacaagu ggaacgccga guacgacgac auccaccuga agaagaaggc gguggucacc     1140

gagaaguacg aggacgaccg gcgcaagagc uucaagaaga ucggguccuu cagccuggag     1200

cagcuccagg aguacgccga cgccgaccug uccguggugg agaagcugaa ggagaucauc     1260

auccagaagg ucgacgagau cuacaaggug uacggcagcu ccgagaagcu guucgacgcc     1320

gacuucgugc ucgagaaguc ccugaagaag aacgacgccg ucguggcgau caugaaggac     1380

cuccuggaca gcgucaagag cuucgagaac uacaucaagg ccuucuucgg cgaggggaag     1440

gagacgaacc gggacgaguc cuucuacggc gacuucgugc uggccuacga cauccuccug     1500

aagguggacc acaucuacga cgccauccgc aacuacguca cccagaagcc guacagcaag     1560

gacaaguuca agcuguacuu ccagaacccc caguucaugg gcggguggga caaggacaag     1620

gagaccgacu accgggccac cauccuccgc uacgggucca aguacuaccu ggcgaucaug     1680

gacaagaagu acgccaagug ccugcagaag aucgacaagg acgacgugaa cggcaacuac     1740

gagaagauca acuacaagcu ccugcccggc cccaacaaga ugcugccgaa gguguucuuc     1800

agcaagaagu ggauggccua cuacaacccc uccgaggaca uccagaagau cuacaagaac     1860

gggacguuca agaagggcga cauguucaac cugaacgacu gccacaagcu caucgacuuc     1920

uucaaggaca gcaucucccg guaccccaag uggagcaacg ccuacgacuu caacuucagc     1980

gagaccgaga aguacaagga caucgcgggg uucuaccgcg aggucgagga gcagggcuac     2040

aagguguccu ucgagagcgc cuccaagaag gagguggaca agcuggucga ggaggggaag     2100

cuguacaugu uccagaucua caacaaggac uucagcgaca agucccacgg cacccccaac     2160

cuccacacca uguacuucaa gcugcuguuc gacgagaaca accacggcca gauccggcuc     2220

agcgggggcg ccgagcuguu caugcgccgc gccucccuga agaaggagga gcucguggug     2280

cacccggcca acagccccau cgcgaacaag aaccccgaca accccaagaa gacgaccacc     2340

cuguccuacg acgucuacaa ggacaagcgg uucagcgagg accaguacga gcugcacauc     2400

ccgaucgcca ucaacaagug ccccaagaac aucuucaaga ucaacaccga ggugcgcgug     2460

cuccugaagc acgacgacaa ccccuacguc aucgggaucg accggggcga gcgcaaccug     2520

cucuacaucg ugguggucga cgggaagggc aacaucgugg agcaguacuc ccugaacgag     2580

aucaucaaca acuucaacgg cauccggauc aagacggacu accacagccu gcucgacaag     2640

aaggagaagg agcgcuucga ggcccggcag aacuggaccu ccaucgagaa caucaaggag     2700

cugaaggccg gguacaucag ccaggugguc cacaagaucu gcgagcuggu ggagaaguac     2760

gacgccguga ucgcgcucga ggaccugaac uccggcuuca agaacagccg cgucaaggug     2820

gagaagcagg ucuaccagaa guucgagaag augcugaucg acaagcucaa cuacauggug     2880

gacaagaagu ccaaccccug cgccaccggg ggcgcccuga agggcuacca gaucaccaac     2940

aaguucgaga gcuucaagag cauguccacg cagaacgggu ucaucuucua caucccggcc     3000

uggcugacca gcaagaucga ccccuccacc ggcuucguga accuccugaa gaccaaguac     3060

acgagcaucg ccgacuccaa gaaguucauc agcuccuucg accggaucau guacgugccc     3120

gaggaggacc uguucgaguu cgcgcucgac uacaagaacu ucagccgcac cgacgccgac     3180

uacaucaaga aguggaagcu guacuccuac gggaaccgga uccgcaucuu cgccgccgcg     3240

aagaagaaca acgucuucgc cugggaggag gugugccuga ccagcgccua caaggagcug     3300

uucaacaagu acggcaucaa cuaccagcag ggggacaucc gggcccuccu gugcgagcag     3360

uccgacaagg ccuucuacag cuccuucaug gcgcucauga gccugaugcu gcagaugcgc     3420

aacuccauca ccggccggac cgacguggac uuccucauca gccccgucaa gaacuccgac     3480

ggcaucuucu acgacagccg caacuacgag gcccaggaga acgccauccu gccgaagaac     3540

gccgacgcca acggggcgua caacaucgcc cggaaggugc ugugggccau cggccaguuc     3600

aagaaggccg aggacgagaa gcucgacaag gugaagaucg ccaucuccaa caaggagugg     3660

cuggaguacg cgcagacgag cgucaaguga                                      3690


<210>  2303
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2303
augagcaucu accaggaguu cgugaacaag uacucccuga gcaagacccu ccgcuucgag       60

cugauccccc agggcaagac ccuggagaac aucaaggccc gggggcucau ccuggacgac      120

gagaagcgcg ccaaggacua caagaaggcg aagcagauca ucgacaagua ccaccaguuc      180

uucaucgagg agauccuguc cagcgucugc aucuccgagg accuccugca gaacuacagc      240

gacguguacu ucaagcugaa gaaguccgac gacgacaacc uccagaagga cuucaagagc      300

gccaaggaca cgaucaagaa gcagaucucc gaguacauca aggacagcga gaaguucaag      360

aaccuguuca accagaaccu gaucgacgcc aagaagggcc aggaguccga ccucauccug      420

uggcugaagc agagcaagga caacgggauc gagcuguuca aggccaacuc cgacaucacc      480

gacaucgacg aggcccucga gaucaucaag agcuucaagg gcuggaccac cuacuucaag      540

ggguuccacg agaaccggaa gaacguguac uccagcgacg acaucccgac guccaucauc      600

uaccgcaucg ucgacgacaa ccugcccaag uuccucgaga acaaggcgaa guacgagagc      660

cugaaggaca aggcccccga ggccaucaac uacgagcaga ucaagaagga ccuggccgag      720

gagcucaccu ucgacaucga cuacaagacc uccgagguga accagcgggu guucagccug      780

gacgaggucu ucgagaucgc gaacuucaac aacuaccuga accagagcgg caucaccaag      840

uucaacacga ucaucggcgg gaaguucgug aacggcgaga acaccaagcg caaggggauc      900

aacgaguaca ucaaccucua cucccagcag aucaacgaca agacccugaa gaaguacaag      960

augagcgugc uguucaagca gauccucucc gacaccgaga gcaaguccuu cgucaucgac     1020

aagcuggagg acgacagcga cguggugacg accaugcagu ccuucuacga gcagaucgcc     1080

gccuucaaga ccgucgagga gaaguccauc aaggagaccc ugagccuccu guucgacgac     1140

cugaaggccc agaagcucga ccugagcaag aucuacuuca agaacgacaa gucccugacg     1200

gaccucagcc agcaggucuu cgacgacuac uccgugaucg gcaccgccgu gcuggaguac     1260

aucacccagc aggucgcgcc caagaaccug gacaacccga gcaagaagga gcaggaccuc     1320

aucgccaaga agaccgagaa ggccaaguac cugucccugg agacgaucaa gcucgcccug     1380

gaggaguuca acaagcaccg ggacaucgac aagcagugcc gcuucgagga gauccuggcc     1440

aacuucgcgg ccauccccau gaucuucgac gagaucgccc agaacaagga caaccucgcc     1500

cagaucagcc ugaaguacca gaaccagggg aagaaggacc ugcuccaggc cuccgcggag     1560

gaggacguga aggccaucaa ggaccugcug gaccagacca acaaccugcu ccaccggcug     1620

aagaucuucc acaucagcca guccgaggac aaggccaaca uccuggacaa ggacgagcac     1680

uucuaccucg uguucgagga gugcuacuuc gagcuggcca acaucgugcc ccuguacaac     1740

aagauccgca acuacaucac ccagaagccc uacagcgacg agaaguucaa gcucaacuuc     1800

gagaacagca cccuggccaa cggcugggac aagaacaagg agccggacaa cacggcgauc     1860

cuguucauca aggacgacaa guacuaccuc ggcgugauga acaagaagaa caacaagauc     1920

uucgacgaca aggccaucaa ggagaacaag ggggagggcu acaagaagau cgucuacaag     1980

cugcugcccg gggccaacaa gaugcucccc aagguguucu ucuccgccaa gagcaucaag     2040

uucuacaacc ccuccgagga cauccugcgg auccgcaacc acagcaccca caccaagaac     2100

ggcaacccgc agaagggcua cgagaaguuc gaguucaaca ucgaggacug ccggaaguuc     2160

aucgacuucu acaaggaguc caucagcaag caccccgagu ggaaggacuu cggguuccgc     2220

uucuccgaca cccagcggua caacagcauc gacgaguucu accgcgaggu ggagaaccag     2280

ggcuacaagc ugacguucga gaacaucucc gagagcuaca ucgacuccgu cgugaaccag     2340

gggaagcucu accuguucca gaucuacaac aaggacuuca gcgcguacuc caagggccgg     2400

cccaaccugc acacccucua cuggaaggcc cuguucgacg agcgcaaccu gcaggacguc     2460

guguacaagc ucaacgggga ggccgagcug uucuaccgga agcagagcau ccccaagaag     2520

aucacccacc cggccaagga ggccaucgcg aacaagaaca aggacaaccc caagaaggag     2580

uccguguucg aguacgaccu gaucaaggac aagcgcuuca ccgaggacaa guucuucuuc     2640

cacugcccca ucacgaucaa cuucaagagc uccggcgcca acaaguucaa cgacgagauc     2700

aaccuccugc ugaaggagaa ggccaacgac guccacaucc ucagcaucga ccggggcgag     2760

cgccaccugg ccuacuacac ccugguggac gggaagggca acaucaucaa gcaggacacc     2820

uucaacauca ucgggaacga ccgcaugaag accaacuacc acgacaagcu cgccgcgauc     2880

gagaaggacc gggacagcgc ccgcaaggac uggaagaaga ucaacaacau caaggagaug     2940

aaggagggcu accuguccca ggugguccac gagaucgcca agcuggugau cgagcacaac     3000

gccaucgugg ucuucgagga ccucaacuuc ggguucaagc ggggccgcuu caagguggag     3060

aagcaggucu accagaagcu ggagaagaug cugaucgaga agcucaacua ccugguguuc     3120

aaggacaacg aguucgacaa gacgggcggg gugcugcggg ccuaccagcu caccgcgccc     3180

uucgagaccu ucaagaagau gggcaagcag accgggauca ucuacuacgu gccggccggc     3240

uucaccagca agaucugccc cgucacgggg uucgugaacc agcuguaccc caaguacgag     3300

uccgugagca agucccagga guucuucagc aaguucgaca agaucugcua caaccuggac     3360

aagggcuacu ucgaguucuc cuucgacuac aagaacuucg gcgacaaggc cgccaagggg     3420

aaguggacca ucgcgagcuu cggcucccgc cucaucaacu uccggaacag cgacaagaac     3480

cacaacuggg acacccgcga ggucuacccc accaaggagc uggagaagcu gcucaaggac     3540

uacuccaucg aguacgggca cggcgagugc aucaaggccg ccaucugcgg cgagagcgac     3600

aagaaguucu ucgccaagcu gacguccgug cugaacacca uccuccagau gcggaacagc     3660

aagaccggga ccgagcugga cuaccugauc uccccggugg ccgacgucaa cggcaacuuc     3720

uucgacagcc gccaggcgcc caagaacaug ccccaggacg ccgacgccaa cggggccuac     3780

cacaucggcc ucaaggggcu gaugcugcuc gaccggauca agaacaacca ggagggcaag     3840

aagcugaacc uggugaucaa gaacgaggag uacuucgagu ucgugcagaa ccgcaacaac     3900

uga                                                                   3903


<210>  2304
<211>  1071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2304
augaagacca acuaccacga caagcuggcc gccaucgaga aggaccgcga gagcgcgcgg       60

aaggacugga agaagaucaa caacaucaag gagaugaagg agggcuaccu cucccaggug      120

guccacgaga ucgccaagcu ggugaucggg uacaacgcca ucguggucuu cgaggaccug      180

aacuucggcu ucaagcgcgg gcgguucaag guggagaagc aggucuacca gaagcucgag      240

aagaugcuga ucgagaagcu gaacuaccuc guguucaagg acaacgaguu cgacaaggcc      300

ggcggggugc ugcgcgccua ccagcugacc gcgcccuucg agacguucaa gaagaugggc      360

aagcagaccg gcaucaucua cuacgugccg gccgacuuca ccagcaagau cugccccguc      420

accggguucg ugaaccagcu cuaccccaag uacgaguccg ugagcaaguc ccaggaguuc      480

uucagcaagu ucgacaagau cugcuacaac cuggacaagg gcuacuucga guucuccuuc      540

gacuacaaga acuucgggga caaggccgcc aagggcaagu ggacgaucgc gagcuucggg      600

ucccggcuga ucaacuuccg caacagcgac aagaaccaca acugggacac ccgggagguc      660

uaccccacca aggagcucga gaagcugcug aaggacuacu ccaucgagua cggccacggc      720

gagugcauca aggccgccau cuacgccgag aacgacaaga aguucuucgc caagcucacc      780

agcauccuga acuccauccu gcagaugcgc aacagcaaga cggggaccga gcucgacuac      840

cugaucuccc cgguggcgga cgugaacggc aacuucuucg acagccggca cgcccccaag      900

aacaugcccc aggacgccga cgccaacggg gccuaccaca ucggccugaa gggccucaug      960

cugcuguacc gcaucaagaa caaccaggac gggaagaagc ucaaccuggu caucaagaac     1020

gaggaguacu ucgaguucgu gcagaaccgg aacaagucca gcaagaucug a              1071


<210>  2305
<211>  3792
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2305
auggaggacu acagcggcuu cgugaacauc uacuccaucc agaagacccu gcgcuucgag       60

cucaagcccg ucgggaagac ccuggagcac aucgagaaga agggcuuccu gaagaaggac      120

aagauccggg ccgaggacua caaggccgug aagaagauca ucgacaagua ccaccgcgcg      180

uacaucgagg agguguucga cagcguccuc caccagaaga agaagaagga caagacgcgg      240

uucuccaccc aguucaucaa ggagaucaag gaguucagcg agcuguacua caagaccgag      300

aagaacaucc cggacaagga gcgccuggag gcccucuccg agaagcugcg gaagaugcug      360

gugggggccu ucaagggcga guucagcgag gagguggccg agaaguacaa gaaccucuuc      420

uccaaggagc ugauccgcaa cgagaucgag aaguucugcg agaccgacga ggagcggaag      480

caggucagca acuucaaguc cuucacgacc uacuucaccg gguuccacag caaccgccag      540

aacaucuacu ccgacgagaa gaaguccacc gccaucggcu accggaucau ccaccagaac      600

cugcccaagu uccucgacaa ccugaagauc aucgagagca uccagcgccg guucaaggac      660

uuccccugga gcgaccugaa gaagaaccuc aagaagaucg acaagaacau caagcugacg      720

gaguacuucu ccaucgacgg cuucgugaac gugcugaacc agaaggggau cgacgcguac      780

aacaccaucc ucggcggcaa gagcgaggag uccggggaga agauccaggg gcugaacgag      840

uacaucaacc uguaccgcca gaagaacaac aucgaccgga agaaccuccc caacgucaag      900

auccuguuca agcagauccu gggcgaccgc gagaccaaga gcuucauccc ggaggccuuc      960

cccgacgacc aguccgugcu caacagcauc accgaguucg ccaaguaccu gaagcuggac     1020

aagaagaaga aguccaucau cgccgagcuc aagaaguucc ugagcagcuu caaccgguac     1080

gagcuggacg gcaucuaccu cgcgaacgac aacucccugg ccagcaucuc cacguuccug     1140

uucgacgacu ggagcuucau caagaagucc gugagcuuca aguacgacga guccgucggg     1200

gaccccaaga agaagaucaa gagcccccuc aaguacgaga aggagaagga gaaguggcug     1260

aagcagaagu acuacaccau cuccuuccug aacgacgcca ucgagagcua cuccaagagc     1320

caggacgaga agcgcgugaa gauccggcuc gaggccuacu ucgccgaguu caaguccaag     1380

gacgacgcga agaagcaguu cgaccugcug gagcgcaucg aggaggccua cgccaucgug     1440

gagccgcucc ugggcgccga guacccccgg gaccgcaacc ugaaggccga caagaaggag     1500

gucgggaaga ucaaggacuu ccucgacagc aucaaguccc ugcaguucuu ccugaagccc     1560

cuccugagcg cggagaucuu cgacgagaag gaccugggcu ucuacaacca gcucgagggc     1620

uacuacgagg agaucgacuc caucgggcac cuguacaaca aggugcggaa cuaccugacc     1680

ggcaagaucu acagcaagga gaaguucaag cugaacuucg agaacagcac ccuccugaag     1740

gggugggacg agaaccgcga gguggccaac cugugcguca ucuuccgcga ggaccagaag     1800

uacuaccucg gcgugaugga caaggagaac aacacgaucc uguccgacau ccccaaggug     1860

aagccgaacg agcuguucua cgagaagaug gucuacaagc ucauccccac gccccacaug     1920

cagcugcccc ggaucaucuu cagcuccgac aaccugagca ucuacaaccc guccaagagc     1980

auccucaaga uccgcgaggc caaguccuuc aaggagggga agaacuucaa gcugaaggac     2040

ugccacaagu ucaucgacuu cuacaaggag agcaucucca agaacgagga cuggagccgg     2100

uucgacuuca aguucuccaa gaccagcucc uacgagaaca ucagcgaguu cuaccgcgag     2160

guggagcggc agggcuacaa ccuggacuuc aagaaggugu ccaaguucua caucgacagc     2220

cucgucgagg acggcaagcu guaccuguuc cagaucuaca acaaggacuu cuccaucuuc     2280

agcaagggga agcccaaccu ccacaccauc uacuuccgca gccuguucuc caaggagaac     2340

cugaaggacg ugugccucaa gcugaacggc gaggccgaga uguucuuccg gaagaagucc     2400

aucaacuacg acgagaagaa gaagcgcgag gggcaccacc ccgagcuguu cgagaagcuc     2460

aaguacccca uccugaagga caagcgguac agcgaggaca aguuccaguu ccaccucccg     2520

aucagccuca acuucaaguc caaggagcgc cugaacuuca accugaaggu caacgaguuc     2580

cucaagcgga acaaggacau caacaucauc ggcaucgacc gcggggagcg gaaccugcug     2640

uaccucguga ugaucaacca gaagggcgag auccugaagc agacccugcu cgacagcaug     2700

caguccggca aggggcgccc cgagaucaac uacaaggaga agcugcagga gaaggagauc     2760

gagcgggaca aggcccgcaa gagcuggggc accguggaga acaucaagga gcugaaggag     2820

ggguaccucu ccaucgucau ccaccagauc agcaagcuga ugguggagaa caacgcgauc     2880

gugguccugg aggaccucaa caucggcuuc aagcgggggc gccagaaggu ggagcgccag     2940

guguaccaga aguucgagaa gaugcugauc gacaagcuga acuuccucgu cuucaaggag     3000

aacaagccca ccgagcccgg cggcgugcug aaggccuacc agcugaccga cgaguuccag     3060

uccuucgaga agcucagcaa gcagacgggg uuccuguucu acgugccguc cuggaacacc     3120

agcaagaucg acccccggac cggcuucauc gacuuccugc accccgccua cgagaacauc     3180

gagaaggcca agcaguggau caacaaguuc gacuccaucc gcuucaacag caagauggac     3240

ugguucgagu ucaccgccga cacgcggaag uucuccgaga accucaugcu ggggaagaac     3300

cgcgucuggg ugaucugcac caccaacgug gagcgguacu ucaccagcaa gacggcgaac     3360

uccagcaucc aguacaacag cauccagauc accgagaagc ugaaggagcu guucgucgac     3420

auccccuucu ccaacggcca ggaccugaag ccggagaucc ugcgcaagaa cgacgccgug     3480

uucuucaaga gccuccuguu cuacaucaag accacccugu cccuccggca gaacaacggc     3540

aagaaggggg aggaggagaa ggacuucauc cugagccccg uggucgacuc caagggccgc     3600

uucuucaaca gccuggaggc cuccgacgac gagcccaagg acgccgacgc gaacggggcc     3660

uaccacaucg cccucaaggg ccugaugaac cugcucgugc ugaacgagac gaaggaggag     3720

aaccugagcc ggccgaagug gaagaucaag aacaaggacu ggcucgaguu cgugugggag     3780

cgcaaccggu ga                                                         3792


<210>  2306
<211>  2820
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2306
augaaccugu acagcaaccu caccaacaag uacucccuga gcaagacccu gcgcuucgag       60

cucauccccc agggcgagac gcuggagaac aucaaggccc gggggcugau ccucgacgac      120

gagaagcgcg ccaaggacua caagaaggcg aagcagauca ucgacaagua ccaccaguuc      180

uucaucgagg agauccuguc cagcgugugc aucuccgagg accugcucca gaacuacagc      240

gacgucuacu ucaagcugaa gaaguccgac gacgacaacc ugcagaagga cuucaagagc      300

gccaaggaca ccaucaagaa gcacaucucc cgguacauca acgacagcga gaaguucaag      360

aaccucuuca accagaaccu gaucgacgcc aagaagggcc aggaguccga ccugauccuc      420

uggcugaagc agagcaagga caacgggauc gagcuguuca aggccaacuc cgacaucacc      480

gacaucgacg aggcccucga gaucaucaag agcuucaagg gcuggaccac guacuucaag      540

ggguuccacg agaaccgcaa gaacguguac uccagcgacg acaucccgac cuccaucauc      600

uaccggaucg uggacgacaa ccugcccaag uucaucgaga acaaggcgaa guacgagaac      660

cugaaggaca aggcccccga ggccaucaac uacgagcaga ucaagaagga ccucgccgag      720

gagcugaccu ucgacaucga cuacaagacc agcgagguca accagcgcgu guucucccug      780

gacgaggugu ucgagaucgc gaacuucaac aacuaccuca accagagcgg caucacgaag      840

uucaacacca ucaucggcgg gaaguucguc aacggcgaga acaccaagcg gaaggggauc      900

aacgaguaca ucaaccugua cagccagcag aucaacgaca agacccugaa gaaguacaag      960

auguccgugc uguucaagca gauccugagc gacacggagu ccaagagcuu cgugaucgac     1020

aagcuggagg acgacuccga cgucgugacc accaugcaga gcuucuacga gcagaucgcc     1080

gccuucaaga cccucgagga gaaguccauc aaggagacgc ugagccuccu cuucgacgac     1140

cugaaggccc agaagcugga ccucuccaag aucuacuuca agaacgacaa gagccugacc     1200

gaccuguccc agcaggucuu cgacgacuac agcgugaucg gcaccgccgu gcucgaguac     1260

aucacccagc aggucgcgcc caagaaccug gacaacccgu ccaagaagga gcaggaccug     1320

aucgccaaga agacggagaa ggccaaguac cucagccugg agaccaucaa gcuggcccuc     1380

gaggaguuca acaaguaccg cgacaucgac aagcagugcc gguucgagga gaucuucgcc     1440

uccuucgcgg acauccccgu gcuguucgac gagaucgccc agaacaagaa caaccuggcc     1500

cagaucagca ucaaguacca gaaccagggg aagaaggacc uccugcagac cuccgccgag     1560

guggacguga aggccaucaa ggaccugcug gaccagacca acaaccuccu gcacaagcug     1620

aagaucuucc acaucacgca gagcgaggac aaggcgaaca uccucgacaa ggacgagcac     1680

uucuaccugg ucuucgacga gugcuacuuc gagcuggcca acaucguggc ccucuacaac     1740

aagauccgca acuacaucac ccagaagccc uacuccgacg agaaguucaa gcugaacuuc     1800

gagaacagca cccuggccaa cggcugggac aagaacaagg agcccgacaa caccgccauc     1860

cucuucauca aggacgacaa guacuaccug ggcgugauga acaagaagaa caacaagauc     1920

uucgacgaca aggcgaucaa ggagaacaag ggggagggcu acaagaaggu cguguacaag     1980

cugcucccgg gggccaacaa gaugcugccc aagguguucu ucagcgccaa guccaucaac     2040

uucuacaacc ccagcgagga cauccugcgg auccgcaacc acuccacgca caccaagaac     2100

ggcagccccc agaagggcua cgagaagcuc gaguucaaca ucgaggacug ccggaaguuc     2160

aucgacuucu acaagcacuc caucagccgc cacccggagu ggaaggacuu cggguuccgg     2220

uucuccgaca ccaagaagua caacagcauc gacgaguucu accgcgaggu cgagaaccag     2280

ggcuacaagc ugaccuucga gaacaucucc gagagcuaca ucgacucccu gguggacgag     2340

gggaagcucu accuguucca gaucuacaac aaggacuuca gcgucuacuc caagggcaag     2400

cccaaccugc acacgcucua cuggaaggcc cuguucgacg agcggaaccu gcaggacgug     2460

guguacaagc ucaacgggga ggcggagcug uucuaccgca agcagagcau ccccaagaag     2520

aucacccacc ccgccaagga ggccaucgcc aacaagaaca aggacaaccc gaagaaggag     2580

uccaucuucg aguacgaccu gaucaaggac aagcgguuca ccgaggacaa guucuucuuc     2640

cacugcccca ucaccaucaa cuucaagagc uccggcgcca acaaguucaa cgacgagauc     2700

aaccuccugc ugaaggagaa ggcgaacgac guccacaucc ucagcaucga ccgcggcgag     2760

cggcaccugg ccuacuacac gcugguggac gggaagggca acaucaucug caagaacuga     2820


<210>  2307
<211>  3795
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2307
augcuguucc aggaguucac ccaccucuac ccccugagca agaccgugcg cuucgagcug       60

aagccgaucg gcaagacgcu cgagcacauc cacgccaagg acuuccuguc ccaggacgag      120

accauggccg acauguacca gaaggucaag gcgauccugg acgacuacca ccgggacuuc      180

aucgccgaca ugaugagcgg gguggugcuc accaagcugg ccgaguucua cgaggucuac      240

cuggcccucc gcaagacccc caaggacgac ucccugcaga agcagcugac ggagauccag      300

accgcccucc ggaaggagau cgugaagccc aucggcagcg ggggcaagua caaggcgggg      360

uacgaccgcc uguucggcgc caagcuguuc aaggacggca aggagcucgg ggaccuggcc      420

aaguucguga ucacccagga gggcgagucc agccccaagc uggcccacau cgcgcacuuc      480

gagaaguucu ccaccuacuu cacgggguuc cacgacaacc ggaagaacau guacagcgac      540

gaggacaagc acaccgccau cgccuaccgc cucauccacg agaaccugcc gcgguucauc      600

gacaaccugc agauccucgc caccaucaag cagaagcacu ccgcccugua cgaccagauc      660

gucaacgagc ugaacgcgaa cggccucgac gugagccugg ccucccaccu ggacggguac      720

cacaagcucc ugacccagga gggcaucacg gccuacaaca cccugcucgg cgggaucagc      780

ggcgaggccg ggucccgcaa gauccagggc aucaacgagc ugaucaacau ccaccacaac      840

cagcacugcc agaaguccga gcggaucgcc aagcugcgcc cccuccacaa gcagauccug      900

agcgacggca ugggggugag cuuccugccc uccaaguucg cggacgacag cgaggucugc      960

caggccguga acgaguucua ccggcacuac gcccacgugu ucgccaaggu ccagucccuc     1020

uucgaccgcu ucgacgacua ccggaaggac ggcaucuacg uggagcacaa gaaccugaac     1080

gagcugagca agcaggccuu cggggacuuc gcgcuccugg gccgcgugcu ggacggguac     1140

uacgucgacg uggugaaccc cgaguucaac gagcgguucg ccaaggccaa gaccgacaac     1200

gccaaggaga agcucaccaa ggagaaggac aaguucauca agggcgucca cagccuggcc     1260

ucccuggagc aggcgaucga gcacuacauc gccggccacg acgacgagag cgugcaggcc     1320

gggaagcucg gccaguacuu caagcacggg cuggccggcg uggacaaccc gauccagaag     1380

auccacaacu cccacagcac gaucaagggg uuccuggagc gcgagcggcc cgcgggcgag     1440

cgcgcccucc ccaagaucaa guccgacaag agccccgaga ugacccagcu gcggcagcug     1500

aaggagcugc ucgacaacgc ccugaacguc gugcacuucg ccaagcugcu caccaccaag     1560

acgacccugg acaaccagga cggcaacuuc uacggggagu ucggcgcccu guacgacgag     1620

cucgcgaaga ucgccacccu guacaacaag gugcgcgacu accuguccca gaagccguuc     1680

agcaccgaga aguacaagcu caacuucggg aaccccacgc ugcugaacgg cugggaccuc     1740

aacaaggaga aggacaacuu cggggucauc cugcagaagg acggcugcua cuaccuggcc     1800

cuccuggaca aggcccacaa gaagguguuc gacaacgccc ccaacgcggg caaguccguc     1860

uaccagaaga ugguguacaa gcugcucccc gggccgaaca agaugcugcc caagguguuc     1920

uucgccaaga gcaaccugga cuacuacaac cccuccgccg agcuccugga caaguacgcc     1980

cagggcaccc acaagaaggg gaacaacuuc aaccugaagg acugccacgc ccucaucgac     2040

uucuucaagg cgagcaucaa caagcacccc gaguggcagc acuucggcuu cgaguucucc     2100

ccgaccagcu ccuaccagga ccugagcgac uucuaccggg aggucgagcc ccagggcuac     2160

caggugaagu ucguggacau caacgccgag uacaucgacg agcuggucga gcaggggcag     2220

cucuaccugu uccagaucua caacaaggac uucuccccca aggcccacgg caagcccaac     2280

cugcacaccc ucuacuucaa ggcccuguuc agcgagaaca accuggcgaa cccgaucuac     2340

aagcucaacg gggaggccga gaucuucuac cgcaaggccu cccuggacau gaacgagacg     2400

accauccacc gcgccggcga ggugcuggag aacaagaacc ccgacaaccc caagaagcgg     2460

caguucgugu acgacaucau caaggacaag cgcuacaccc aggacaaguu caugcuccac     2520

guccccauca ccaugaacuu cggggugcag ggcaugacga ucaaggaguu caacaagaag     2580

gugaaccaga gcauccagca guacaacgac gucaacguga ucggcaucga ccgcggggag     2640

cggcaccucc uguaccugac cgugaucaac agcaagggcg agauccugga gcagcggucc     2700

cugaacgaca ucaucaccac cagcgccaac gggacgcaga ugaccacccc guaccacaag     2760

auccucgaca agcgcgagau cgagcggcug aacgcgcgcg ucggcugggg cgagaucgag     2820

accaucaagg agcugaaguc cggguaccuc agccacgugg ugcaccagau cucccagcug     2880

augcugaagu acaacgccau cgucgugcuc gaggaccuga acuucggcuu caagcggggg     2940

cgcuucaagg uggagaagca gaucuaccag aacuucgaga acgcccugau caagaagcuc     3000

aaccaccugg cccugaagga caaggccgac gacgagaucg gcagcuacaa gaacgcgcuc     3060

cagcugacca acaacuucac ggaccugaag uccaucggga agcagaccgg cuuccucuuc     3120

uacguccccg ccuggaacac cagcaagauc gaccccgcca ccggguucgu ggaccugcug     3180

aagccgcggu acgagaacau cgcccagucc caggccuucu ucggcaaguu cgacaagauc     3240

uucuacaacg cggacaaggg cuacuucgag uucuacgugg acuacgccaa guucacggac     3300

aaggccaaga acagccgcca gaccuggguc aucugcuccc acggggacaa gcgguacgug     3360

uacgacaaga ccgccaacca gaacaagggc gcgaccaucg ggaucaacgu gaacgacgag     3420

cucaagagcc uguucgcccg cuaccacauc aacgacaagc agcccaaccu ggucauggac     3480

aucugccaga acaacgacaa ggaguuccac aagucccuca uguaccugcu gaaggcccuc     3540

cuggcccugc gguacagcaa cgccuccagc gacgaggacu ucauccucuc ccccguggcg     3600

gacgacaagg gcguguucuu caacagcgcc cuggccgacg acacgcagcc ccagaacgcc     3660

gacgccaacg gggcguacca caucgcccug aagggccucu ggcugcugaa cgagcucaag     3720

aacagcaacg accuggacaa gaucaagcug gccaucgaca accagaccug gcucaacuuc     3780

gcccagaacc gcuga                                                      3795


<210>  2308
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2308
augagcaucu accaggaguu cgugaacaag uacucccuga gcaagacccu ccgcuucgag       60

cugauccccc agggcaagac ccuggagaac aucaaggccc gggggcucau ccuggacgac      120

gagaagcgcg ccaaggacua caagaaggcg aagcagauca ucgacaagua ccaccaguuc      180

uucaucgagg agauccuguc cagcgucugc aucuccgagg accuccugca gaacuacagc      240

gacguguacu ucaagcugaa gaaguccgac gacgacaacc uccagaagga cuucaagagc      300

gccaaggaca cgaucaagaa gcagaucucc gaguacauca aggacagcga gaaguucaag      360

aaccuguuca accagaaccu gaucgacgcc aagaagggcc aggaguccga ccucauccug      420

uggcugaagc agagcaagga caacgggauc gagcuguuca aggccaacuc cgacaucacc      480

gacaucgacg aggcccucga gaucaucaag agcuucaagg gcuggaccac cuacuucaag      540

ggguuccacg agaaccggaa gaacguguac uccagcgacg acaucccgac guccaucauc      600

uaccgcaucg ucgacgacaa ccugcccaag uuccucgaga acaaggcgaa guacgagagc      660

cugaaggaca aggcccccga ggccaucaac uacgagcaga ucaagaagga ccuggccgag      720

gagcucaccu ucgacaucga cuacaagacc uccgagguga accagcgggu guucagccug      780

gacgaggucu ucgagaucgc gaacuucaac aacuaccuga accagagcgg caucaccaag      840

uucaacacga ucaucggcgg gaaguucgug aacggcgaga acaccaagcg caaggggauc      900

aacgaguaca ucaaccucua cucccagcag aucaacgaca agacccugaa gaaguacaag      960

augagcgugc uguucaagca gauccucucc gacaccgaga gcaaguccuu cgucaucgac     1020

aagcuggagg acgacagcga cguggugacg accaugcagu ccuucuacga gcagaucgcc     1080

gccuucaaga ccgucgagga gaaguccauc aaggagaccc ugagccuccu guucgacgac     1140

cugaaggccc agaagcucga ccugagcaag aucuacuuca agaacgacaa gucccugacg     1200

gaccucagcc agcaggucuu cgacgacuac uccgugaucg gcaccgccgu gcuggaguac     1260

aucacccagc aggucgcgcc caagaaccug gacaacccga gcaagaagga gcaggaccuc     1320

aucgccaaga agaccgagaa ggccaaguac cugucccugg agacgaucaa gcucgcccug     1380

gaggaguuca acaagcaccg ggacaucgac aagcagugcc gcuucgagga gauccuggcc     1440

aacuucgcgg ccauccccau gaucuucgac gagaucgccc agaacaagga caaccucgcc     1500

cagaucagcc ugaaguacca gaaccagggg aagaaggacc ugcuccaggc cuccgcggag     1560

gaggacguga aggccaucaa ggaccugcug gaccagacca acaaccugcu ccaccggcug     1620

aagaucuucc acaucagcca guccgaggac aaggccaaca uccuggacaa ggacgagcac     1680

uucuaccucg uguucgagga gugcuacuuc gagcuggcca acaucgugcc ccuguacaac     1740

aagauccgca acuacaucac ccagaagccc uacagcgacg agaaguucaa gcucaacuuc     1800

gagaacagca cccuggccaa cggcugggac aagaacaagg agccggacaa cacggcgauc     1860

cuguucauca aggacgacaa guacuaccuc ggcgugauga acaagaagaa caacaagauc     1920

uucgacgaca aggccaucaa ggagaacaag ggggagggcu acaagaagau cgucuacaag     1980

cugcugcccg gggccaacaa gaugcucccc aagguguucu ucuccgccaa gagcaucaag     2040

uucuacaacc ccuccgagga cauccugcgg auccgcaacc acagcaccca caccaagaac     2100

ggcaacccgc agaagggcua cgagaaguuc gaguucaaca ucgaggacug ccggaaguuc     2160

aucgacuucu acaaggaguc caucagcaag caccccgagu ggaaggacuu cggguuccgc     2220

uucuccgaca cccagcggua caacagcauc gacgaguucu accgcgaggu ggagaaccag     2280

ggcuacaagc ugacguucga gaacaucucc gagagcuaca ucgacuccgu cgugaaccag     2340

gggaagcucu accuguucca gaucuacaac aaggacuuca gcgcguacuc caagggccgg     2400

cccaaccugc acacccucua cuggaaggcc cuguucgacg agcgcaaccu gcaggacguc     2460

guguacaagc ucaacgggga ggccgagcug uucuaccgga agcagagcau ccccaagaag     2520

aucacccacc cggccaagga ggccaucgcg aacaagaaca aggacaaccc caagaaggag     2580

uccguguucg aguacgaccu gaucaaggac aagcgcuuca ccgaggacaa guucuucuuc     2640

cacugcccca ucacgaucaa cuucaagagc uccggcgcca acaaguucaa cgacgagauc     2700

aaccuccugc ugaaggagaa ggccaacgac guccacaucc ucagcaucga ccggggcgag     2760

cgccaccugg ccuacuacac ccugguggac gggaagggca acaucaucaa gcaggacacc     2820

uucaacauca ucgggaacga ccgcaugaag accaacuacc acgacaagcu cgccgcgauc     2880

gagaaggacc gggacagcgc ccgcaaggac uggaagaaga ucaacaacau caaggagaug     2940

aaggagggcu accuguccca ggugguccac gagaucgcca agcuggugau cgagcacaac     3000

gccaucgugg ucuucgagga ccucaacuuc ggguucaagc ggggccgcuu caagguggag     3060

aagcaggucu accagaagcu ggagaagaug cugaucgaga agcucaacua ccugguguuc     3120

aaggacaacg aguucgacaa gacgggcggg gugcugcggg ccuaccagcu caccgcgccc     3180

uucgagaccu ucaagaagau gggcaagcag accgggauca ucuacuacgu gccggccggc     3240

uucaccagca agaucugccc cgucacgggg uucgugaacc agcuguaccc caaguacgag     3300

uccgugagca agucccagga guucuucagc aaguucgaca agaucugcua caaccuggac     3360

aagggcuacu ucgaguucuc cuucgacuac aagaacuucg gcgacaaggc cgccaagggg     3420

aaguggacca ucgcgagcuu cggcucccgc cucaucaacu uccggaacag cgacaagaac     3480

cacaacuggg acacccgcga ggucuacccc accaaggagc uggagaagcu gcucaaggac     3540

uacuccaucg aguacgggca cggcgagugc aucaaggccg ccaucugcgg cgagagcgac     3600

aagaaguucu ucgccaagcu gacguccgug cugaacacca uccuccagau gcggaacagc     3660

aagaccggga ccgagcugga cuaccugauc uccccggugg ccgacgucaa cggcaacuuc     3720

uucgacagcc gccaggcgcc caagaacaug ccccaggacg ccgacgccaa cggggccuac     3780

cacaucggcc ucaaggggcu gaugcugcuc gaccggauca agaacaacca ggagggcaag     3840

aagcugaacc uggugaucaa gaacgaggag uacuucgagu ucgugcagaa ccgcaacaac     3900

uga                                                                   3903


<210>  2309
<211>  3756
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2309
augaaccaca ugaagcaguu caccaaccag uucagccugu ccaagacccu ccgcuucgag       60

cugauccccc agggcaagac gaaggaguuc aucgagauca acgggcugau cgagaaggac      120

aacgagcggg ccgugagcua caagaagguc aagaagauca ucgacgagua ccacaaguac      180

uucaucgaga uggugcucug cgacuucaag cugcacggcc uggagaccua cgagaccauc      240

uucaacaaga aggagaagga cgacaccgac aagaaggagu ucgacaacau ccgcaacucc      300

cuccggaagc agaucgccga cgcguucgcc aagaacccga acgacgagau caaggagcgc      360

uucaagaacc uguucgccaa ggagcugauc aagcaggacc uccugaacuu cguggacgac      420

gagcagaagg agcuggucaa cgaguucaag gacuucacga ccuacuucac cggguuccac      480

cagaaccggc gcaacaugua cguggccgac gagaaggcca ccgcgaucgc cuaccggcuc      540

gugaacgaga accugcccaa guucaucgac aaccugaaga ucuacgagaa gaucaagaag      600

gacgcccccg agcucaucag cgaccugaac aagacgcugg ucgagaugga ggagaucgug      660

cagggcaaga cccucgacga gaucuucucc cugagcuucu ucaaccagac ccugacccag      720

acggggaucg agcucuacaa caucgugauc ggcggccgca ccgccgacga ggggaagacc      780

aagaucaagg gccugaacga guacaucaac accgacuaca accagaagca gacggacaag      840

aagaagaagc aggcgaaguu caagcagcug uacaagcaga uccucuccga ccggcacagc      900

gucuccuucg uggccgagac cuucgagacc gacgcccagc ugcuggagaa caucgagcag      960

uucuacagcu ccgugcucug caacuacgag gacgacgggc acaccacgaa caucuucgag     1020

gccaucaaga accugaucau cggccugaag accuucgacc ucagcaagau cuaccugcgc     1080

aacgacaccu cccugaccga caucagccag aagcuguucg gggacugguc caucaucagc     1140

uccgcccuga acgacuacua cgagaagcag aaccccauca gcuccaagga gaagcaggag     1200

aaguacgacg agcggaaggc gaaguggcug aagcaggacu ucaacaucga gacgauccag     1260

accgcccuca acgagugcga cagcgagauc aucaaggaga agaacaacaa gaacaucguc     1320

agcgaguacu ucgccaagcu gggccuggac aaggacaaca agaucgaccu ccugcagaag     1380

auccaccaca acuacguggu gaucaaggac cugcucaacg agccguaccc cgagaacauc     1440

aagcugggca accagaagga gcaggucucc cagaucaagg acuuccugga cagcauccuc     1500

aaccugaucc acuuccugaa gccccucucc cugaaggaca aggacaagga gaaggacgag     1560

cuguucuaca gccuguucac cgcccucuuc gagcaccugu cccagaccau cagcaucuac     1620

aacaaggugc gcaacuaccu gacgcagaag gccuacucca ccgagaagau caagcucaac     1680

uucgagaaca gcacccugcu gaacgggugg gacgugaaca aggagcccgu caacaccucc     1740

gugaucuucc ggaagaacgg ccucuucuac cuggggauca ugagcaaguc caacaaccgc     1800

aucuucgagc ggaacgugcc ggucugcaag aacgaggaga cggcguucga gaagaugaac     1860

uacaagcugc uccccggcgc caacaagaug cugcccaagg uguuccugag cgccaagggc     1920

aucgaguccu uccagcccag cgccgagauc caguccaagu accagaagga gacccacaag     1980

aagggggacg ccuucgugcg caaggacaug gagaaccuca ucgacuucuu caagcagagc     2040

aucgcgaagc acaccgacug gaagcacuuc aaccaccagu ucuccaagac cgagacguac     2100

aacgaccuga gcgaguucua caaggagguc gagaagcagg gcuacaagcu gaccuucacc     2160

aagcucgacg agaccuacau caaccagcug guggacgagg ggaagcugua ccucuuccag     2220

aucuacaaca aggacuucuc cccguucagc aagggcaagc ccaacaugca cacccuguac     2280

uggaagaugc uguucgacga gcagaaccuc cagaacgucg uguacaagcu gaacggggag     2340

gccgaggugu ucuuccggca gagcuccauc aagcagacgg accgcaucau ccacaaggcc     2400

aaccaggcca ucgacaacaa gaacccccug aacaacaaga agcagagcuc cuucaacuac     2460

gaccucauca aggacaagcg guucacccug gacaaguucc aguuccacgu ccccaucacc     2520

cugaacuuca aggccgaggg caacgaguac cucaacacca aggugaacga guaccucaag     2580

agcaacuccg acgugaagau caucggccug gaccgcgggg agcggcaccu caucuaccug     2640

acgcugauca accagaaggg cgagcuccug aagcagcaga gccugaacgu caucgcgacc     2700

ucccaggagc acgagaccga cuacaagaac cuccugguga acaaggagaa cgagcgcgcc     2760

aacgcccgcc aggacuggaa gaccaucgag acgaucaagg agcugaagga gggguaccuc     2820

agccaggugg uccaccagau cgccaccaug augguggacg agaacgcgau cguggucaug     2880

gaggaccuga acgccggcuu caugcggggg cgccagaagg uggagcgcca gguguaccag     2940

aagcuggaga agaugcucau cgagaagcug aacuaccugg ucuucaagaa caacgacgug     3000

aacgagaccg ccggcgugcu caacgcccug cagcugacca acaaguucga guccuucgag     3060

aagaugggca agcagagcgg guuccucuuc uacgucccgg ccuggaacac guccaagauc     3120

gaccccgcga ccggcuucgu ggacuuccug aagcccaagu acgagagcgu ggagaaggcc     3180

aagcuguucu ucgagaaguu cgaguccauc aaguucaacg ccgacaagaa cuacuucgag     3240

uucgaguucg acuacaagaa guucaccgag aaggccgagg ggagccagac caaguggacg     3300

gucugcaccc acuccgacgu gcgguaccgg uacaaccccc agaccaaggc cagcgacgag     3360

gugaacguca ccaacgagcu caagcugauc uucgacaagu ucaagaucga guacaagaac     3420

ggcaagaacc ugaagacgga gcuccugcug caggacgaca agcagcuguu cuccaagcuc     3480

cugcacuacc ucgcgcugac ccugaugcuc cgccagagca agagcgggac cgacaucgac     3540

uucauccugu ccccgguggc caagaacggc guguucuacg acagccggaa cgccaugccc     3600

aaccugccca aggacgccga cgccaacggc gcguuccaca ucgcccucaa ggggcugugg     3660

ugcguccagc agaucaagaa ggccgacgac cugaagaaga ucaagcucgc caucuccaac     3720

aaggaguggc ugagcuucgu gcagaaccug aaguga                               3756


<210>  2310
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2310
augaucgaca acaccaagga gaagaaggag ggcagcgugu ucgacggguu cacccgcaag       60

uaccagcugu ccaagacgcu ccgguucgag cugcgcccca uccugaacac cccgaagaug      120

cucgacgacg agcaggucau caagaacgac gagacccggc gcaagaagua cgaggccgug      180

aagcccuggu ucgaccagcu gcaccgggag uucaucgagg acgcccucaa gagcuucaag      240

uucaagaacc ucgcgaucua ccaggacacc uuccagacgu ggcagaagga ccgcaagucc      300

aagcagaaga aggacacccu ggugaagauc gaggucggcc ugcgggagga gaucgugcgc      360

cgguucgagg agguggccaa caucuggguc cgcagcgagc aguacaagcu ccuggggauc      420

aagaaggagg gccuggggau gcuguucgag gccggcgugu uccggcugcu gaaggagcgc      480

uucaagaacg agaaggacac caccguggac ggcaacaaca ucuucgacga guggacgcgg      540

uggaccgggu acuucaagaa guucuucgag acccgcaaga acuucuacaa guccgacgac      600

accagcacgg ccaucgccua ccgggucauc aaccagaacc uccgccgguu cugcgagaac      660

auccagaucu ucgagaagau cuccgagaag aucgaguuca gcgaggugga gaaguccuuc      720

gacaucagcu gcgcgggcau cuucucccug gccuacuaca acgccugccu gcuccagggc      780

gggaucgaca ccuacaacaa gaucaucggg ggcgaggugg acgagaagga caagaagauc      840

cccggcauca acgagcugau caacaaguac cgccaggaca acagcgggga gaagaucccc      900

uuccugaagc agcucgacaa gcagauccac uccgccaagg aggcguucau cgagagcauc      960

gagaccaaca aggagcuggu cggcaagcug aagaccuucu acgagaacgc cgaggugaag     1020

auccaguccu uccggaaccu caucgccgac aucgugacgg acuacagcgg guacgacauc     1080

gacaagaucu accugaccaa ggaggccguc ucccacaacg ccagccgcug guucgcgucc     1140

uucgagagcu ucgagcggga ccuguucgcc gugguggccg agaagcagaa caagcucguc     1200

uacgagcugc ugcgcaccca caagaacgac agcaagaucu ccgacaagga cggcaaguuc     1260

agcuucccgg acuucaucaa gugcucccac aucaagcgcg cccucgagaa gcaggagggc     1320

cggaucugga agggggagua cuacgaggac aucguggacu ucgagaagau caaggacgug     1380

uucacccagu uccugugcgu cuucaaguuc gagcuggagc agcaguucuu ccgcaagacg     1440

accagcgccc agaccggcga gcagaccaag aucggguacg agaucuucgu gacgaagauc     1500

aacgagcuca ucacccggga gaaccccgug aucgaccugg aggagaagau cgcgaucaag     1560

aacuucgccg acgccacccu gcucaucuac cagaucgcca aguacuucgc cgucgagaag     1620

cgccggggcu ggcuggacaa cuacgaccug gacgaccgcu ucuacaaguc cagcgacauc     1680

ggguaccuca acuucuaccg ggacgcguuc gagcagaucg ugcgccccua caaccuguuc     1740

cggaacuacc ugaccaagaa gcccuacaac acgaacaagu ggguccucuc cuucgagaac     1800

ccgacccugg ccgacggcug ggacaagaac aaggagaaga ccaacgccgc cgugauccug     1860

cgcaaggacg gccgguacua ccucgggauc aucaaggagg acugcaagag ccuguucgcc     1920

gaccgcuacu ccaaggagau gagcgagggc aucgaguccg ggagcuucca gaagauggcg     1980

uacaaguucu uccccgaggc cuccaagaug auccccaagu gcagcaccca gacgaagaac     2040

gugaaggagc acuuccggaa guccagcucc gacuacaacc uguuccacga gaaggacuac     2100

aagaucagcg ucgccaucac caagaacauc uacgagcuca acaacguguu cuaccgcaag     2160

gacaacaucg aggaguccuu cgugcccaag aacgacuucg agaagaagcu gggcgucaag     2220

aaguuccagc ggcaguaccu ggagaucagc cgcgacaaca acggguacaa gcaggcccug     2280

gcgcagugga ucgaguucug cauccgguuc cucaaggccu acaagagcac caccaucuuc     2340

gacuacuccc gccugcggga ggccaaggag uacgagagcc uggacgccuu cuaccaggac     2400

aucaacgccc ucacguacaa caucuccuuc gugccgauca gcgagcagua caucaaggag     2460

aagaacgaca acggcgagcu guuccuguuc gagaucuaca acaaggacug gucccucggc     2520

cccauggaca agaaccgcaa gcgcaccaag aaccugcaca cccuguacuu cgagcagcug     2580

uucagcaagg agaacgagca ggagaacuuc cucuuccagc ugaacgggga ggcggagcug     2640

uucuuccggc ccaagaccga ggagaagcgc cucggcuaca agguguggga cgccggggag     2700

aagaaguggg ucaaggccaa ggagaaggag gacggcgccg ugaucgaccg gaagcgcuac     2760

gccaaggaca ucauccuguu ccacugcccc aucacccuca accggguguc cgagagcaag     2820

acgaagcgcg agauggacgu cgagauccgg gaggugcugu ccagcacccc gggggugcac     2880

aucaucggcg ucgaccgcgg cgagaagcac cuggcguacu acuccgugau cgaccagaac     2940

gggaagauca ucgagaccga cacccucaac agcaucggca aggacgggcg gggcaagccc     3000

guggaguacg ccuccaagcu ggagaagcgc gcccaggagc gggaggccag ccgccgggac     3060

ugggaggagg ucgaggccau caaggaccug aagaagggcu acaucuccca ggugauccgc     3120

aaccucgcgg accugaucau caagcacaac gccaucaucg uguucgagga ccugaacaug     3180

cgguucaagc agauccgcgg gggcaucgag aaguccgccu accagcagcu cgagcgggcc     3240

cugaucgaca agcugagcuu ccucgucaag aagggggagg aggaccccaa gcagacgggc     3300

cacauccugc gcgcguacca gcuggccgcc cccgugaucg ccuucaagga cauggggaag     3360

cagaccggcc ucaucuucua cacccaggcc ggcuacacca gcaagacgug cccggagugc     3420

ggguaccggc gcaacaucaa gugccuguuc gagaacaucg agcaggcgaa gacccugauc     3480

gagaaccucg agagcaucaa cuacaacaag aaggaggacg uguuccagau cuccuacagc     3540

cuggagaagc uguccagcaa ggaccagaag aaggagaaga aggucuccaa cgagcucuac     3600

gccaagaccc ugaagaagga caucuucauc cugaccacga agaacgcccu ccgguacaag     3660

ugguacgacc gcuacagcga gaaggccaag guggccaagc gcggcaucga cgaguacaag     3720

ggggagguga acgaguccga gaccaagaag ggcgucguga aggaguucaa ccugaccgag     3780

uaccugaagg ggcuccugaa gaccuacgag aucgacuacg agcacggcgg cauccgggag     3840

cagauccuga gcguggcgcg cgggcgggag uucuacaagg acuuccucua cgcccuguuc     3900

cugcucacgg agacccgcca cuccaucagc ggccggaaca ccgacuacau ccagugcccc     3960

gagugcgagu ucgacucccg caagggguuc aaggacauca aggaguucaa cggcgacgcc     4020

aacggggccu acaacaucgc ccggaagggc aucaugaucc uggagaagau caagcaguuc     4080

aagaaggaca acgacggcaa ccuggagaag augggguggg gcgaccucag caucuccauc     4140

gaggaguggg acaaguucac ccagaaggag uga                                  4173


<210>  2311
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2311
augaacgccg agaagaagug cgccuucaac gaguucacca accuguacga gcucagcaag       60

acccugaagu ucgagcugaa gcccgugggc aagacgaagg agaucaucaa gaacuuccgc      120

gaggagcucg agaagaacaa cccgauccag cuggacaagg agaucuccau cgcgaagcag      180

gagaucaaga aggaccugga cauccuccac cgggaguucg uccgccagag cuucgagcug      240

gccuucaagg agaacaagau caacuacgag aucuucuucg agaucuaccg gaacuucaag      300

aaguucucca agaacaagaa guccgacgag uacaucgccg ccaucaagaa ccugaaggag      360

gagaagauca agcucgugaa gaagaucgcc gacuacuucg ugaagaugga ccaggagugg      420

cgcaaguacu accccagcga gaagaagucc uuccugcuga ugagcaacgg ggucaucaac      480

cuccugaucg acaucaacaa ggaccggaug gacuacagcu ucauccccaa ggaggugugc      540

gagaagauca agggcaaggg gaagaucgag cuguacgaga aguucaacaa gcacuucggc      600

uaccucucca aguacaacac cgcgcgcgag aacuucuaca gcgacgacgu gaagaacucc      660

gccagcgucg ccuaccgggc caucugcgag aaccuggaga aguucugcga caacaagauc      720

aagguggagg cggacaacuu ccccaaggag aucaaggaga aguuccugga guacuucaac      780

aagaucaccg accucuccuu cuacaaccgc ugccugagcc agaacgggau cgaggaguac      840

cacaaguuca aggacgagau caaccggucc aucaacgagc uguacccgaa caacagcaag      900

aacaagcccc agaacauccc cuaccucucc aagcuggaca agcagauccu gagcgagaag      960

gacaagcagu ccuggaucga ggagggcuuc aucgacgaga acaacuucau cuucgaguuc     1020

gagaaguuca ucaagaugaa cgacaaguac auccccagcg agaagaagcu guucaacgag     1080

uucuucaucu uccagaacaa cgacgaggug cuccagaagc uguuccuccu gaagaagacc     1140

aucaacaacu ucgccaacau cuaccuggac gacccgaaga accucaugga ccugaugccc     1200

agcaagggca cgaagaagcg cgacuaccug ucccuccugg agaucaagga ggcccugaac     1260

agcuacgccg agaaccagaa caucggggag acccagaucu ucaagaucaa guacgacgac     1320

gucaucgaca agaccaagac ccccuucgag ugcuucauga agaucuggaa guacgaguac     1380

gaccuccugu acaacgaggg cgacgggaag aagaacaucg aggagggcug ggagaagauc     1440

ugcgagaagc ugcagaacaa gaaggacgag uucacggggg acuacggcaa guacaagaag     1500

gagaucaagg acuacugcga gauggcccuc ucccugagcc agcggaucaa gcaccuggac     1560

auccgcauca agaagcggga cggcuccgag gacgcggaca acaaccucuu cgagggggug     1620

gagaagcugg acgaguucua cgaccgccug uucgagaucg uggacgagca caacgccuac     1680

caguaccacc acgaguucca ggccuuccuc accaagaagc ccuacaagac cgacaaguuc     1740

aagcugagcu ucgacaaccc gcagcugcuc aagggcuggg accugaacaa ggagcccgac     1800

uacuucgggg cccugcuggu caagaacgag aaguacuucc ucgccguguu ccacaagcag     1860

uacgcgaacg agaucuuccg gcagaagaac aagaacucca ccgugaagau caaggaggcc     1920

uacgacgucu ucaacggcaa cuacuacaag aagguggagc ugaagcagau ccccaagcug     1980

uucaugaacc ucccccgcau cugcuucgcc aagagcaacc aggaggaguu cggccugacg     2040

aucgagcugg agaagaucaa guccgaguuc gacaaguucc agaagcucaa gaaggagcgg     2100

caggacaaca accugaaguu cgaccaggag aaguucauca agcugcucgc cuacuaccgc     2160

cacugccugg agauccggga ggacuggaag guguucaagu acaacuucaa gccgcuguac     2220

aaguacagca accucgggga guucuaccgc gaccuggaga aggacaccua caaccuggac     2280

cucguccggg ugaacuccga guacguggag aagaagcgca acgagggcaa gcuguaccug     2340

uucgaggucu acaacaagga cuucagcgac aaguccaucg ggcgcaagaa cauccacagc     2400

gacuacuuca agucccucuu ccugcccgac aaccugacca acaagaagau cgcccucgcg     2460

gccgacgccg agaucuucuu ccgggagaag ucccugaaga acaaggacga gaucaucacc     2520

cagaagaaca agaucaagau cgacaagaac aaccacaagg gcgagaaggc cuacagcaag     2580

aaccgcuaca cggacgacaa gaucauccug caccucccca ucaaccugaa cuucggggag     2640

aacaagaacc acaaggacuu caacuacgag aucaacgaca gccugcucaa gaacaaccgg     2700

gacaucaaga ucaucggcau cgaccgcggc gagaaguacc ugcuguacua cuccgugauc     2760

gaccagaagg gggacaucaa ggagaugggc gacuucaacg uggucaacgg gguggacuac     2820

uacagcaagc ucgucgccua cgaggagaag cggaagcagg cgcgccugga cuggggcgac     2880

auccagagca ucaaggagcu gaaggagggg uacaucuccc acgugaucca cgagaucugc     2940

gaccucauca agaaguacaa cgccaucgug guccuggagg accugagcau gggcuucaag     3000

cggggccgcu ucuuccggga gcgccaggug uaccagaagu ucgagcucgc ccuguuccgg     3060

aagcugaacu acugggugga caagaacaag gaggaggggg agaagggcuc cgcccucagc     3120

gcguggcagc ugacccccaa cgagaaggac uucaaggucu cccagcugga gaaggcccgc     3180

cagcucggga uccuguucua caccgccgcc ggcuacacca gcaagacgug cccggacugc     3240

ggguuccgga agaacaucac ccugaaguac gugaacgaca agcagcucaa ggaggaccug     3300

ggcaagcuga agaucaacuu cguggaggac cacuucaaga ucaaguacaa ccuccuggac     3360

uucgagaaga acaagaaguc cgagaaggcc aaggacaccg uccugcccaa ggaggagcgc     3420

aagaucagcu ucguggcgaa cuccaaggug auccggaucc gcuggcacga caucaagacc     3480

aagcucgcca agcgggagcu ggacggcgag aagaucauca agaaguuccc caaggggguc     3540

aucaaggagu acgacaucaa caucugccug aagcgcauca ucggcgacaa gaucgacuuc     3600

agcaaggacu ccaucacgga gcagcucauc aacgggcggu ucggcaagga cuucuacugg     3660

gagcugaugu acuuccugaa ccucauccug gagauccgca acguggugag cgagaccgag     3720

aucgacuaca uccagugccc cgucugcggc uuccacuccg acgacggguu ccgcggcaac     3780

aaguucaacg gggacgccaa cggcgccuac aacaucgccc ggaaggggcg ccugauccuc     3840

gagaagauca accagcacaa gaaccugaag gagcugaaga ccggcgaccu cagcgugaac     3900

aucguggagu gggacaaggc ggucaacaac ugggacaagu ucgugcaggg cgacaacacc     3960

gaguccaaga acaucgagau cccggccaag gaggagauga ucagcgugga gguccgggag     4020

uccuga                                                                4026


<210>  2312
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2312
augaagaagg agaaggaguu caagagcuuc ggcgacuuca ccaaccugua cgagaucucc       60

aagacccucc gcuucgagcu gaagcccgug gagaacacgc agaccaugcu ggacgaggcc      120

gacgucuucg ggaaggacaa ggugaucaag gacaaguaca ccaagaccaa gccguucauc      180

gacaagcucc accgggaguu cguggacgag agccugaagg acgucucccu gagcggccuc      240

aagaaguacu ccgaggugcu ggagaacugg aagaagaaca agaaggacaa ggacaucgug      300

aaggagcuga agaaggagga ggagcgccuc cggaaggagg ucguggaguu cuucgacaac      360

acggccaaga agugggcgaa cgagaaguac aaggagcugg ggcugaagaa gaaggacauc      420

ggcauccucu ucgaggagag cguguucgac cugcugaagg agaaguacgg ggaggagcag      480

gacuccuucc ucaaggagga gaagggcgac uuccugaaga acgagaaggg cgagaagguc      540

agcaucuucg acgaguggaa gggguucgug ggcuacuuca ccaaguucca ggagacccgc      600

aagaacuucu acaagaacga cgggaccgag acggcccugg ccacccggau caucgaccag      660

aaccucaagc gcuucugcga caacaucgac gacuucaaga agaucaagaa caagaucgac      720

uucuccgagg uggagaagaa cuucaacaag accgccgacg ucuucagccu ggacuucuac      780

aaccagugcc ugcuccagaa gggcaucgac uccuacaacg aguucaucgg gggcaagacc      840

cuggagaacg gcaagaagcu gaagggggug aacgagcucg ugaacgagua ccggcagaag      900

aacaagaacg agaaggucag cuuccugaag cugcucgaca agcagauccu guccgagaag      960

gagaagcuga gcuucggcau cgagaacgac gagcagcucc ugguggugcu gaacuccuuc     1020

uacgagacgg ccgaggagaa gaccaagauc cuccgcaccc uguucgggga cuucgucgag     1080

cacaacgaga acuacgaccu ggacaagacc uacaucagca agguggcguu caacacgauc     1140

ucccacaagu ggaccaacga gacccacaag uucgaggagc uccuguacgg cgccaugaag     1200

gaggacaagc ccaucggccu gaacuacgac aagaaggagg acagcuacaa guuccccgac     1260

uucaucgccc ucggguaccu gaagaagugc cugaacaacc ucgacugcga caccaaguuc     1320

uggaaggaga aguacuacga gaacaacgcc gacaagaagg acaaggacaa gggcuuccug     1380

accgggggcc agaacgcgug ggaccaguuc cugcagaucu ucaucuucga guucaaccag     1440

cuguucaacu ccgaggccuu cgacaacaag gggaaggaga ucaagaucgg cuacgacaac     1500

uuccggaagg acuucgagga gaucaucaac cagaaggacu ucaagaacga cgagaaccuc     1560

aagaucgcca ucaagaacuu cgccgacagc gugcugugga ucuaccagau ggccaaguac     1620

uucgcgaucg agaagaagcg cggcugggac gacgacuucg agcucagcga guucuacacg     1680

aaccccucca acggguacag ccuguucuac gaccgggccu acgaggagau cguccagaag     1740

uacaacgacc ugcgcaacua ccugaccaag aagccguaca aggaggacaa guggaagcuc     1800

aacuucgaga accccacccu ggccaacggc uucgacaaga acaaggaguc cgacaacagc     1860

acggugaucc ugcggaagaa gcgcaaguac uaccucgggc ugaugaagaa gggcaacaac     1920

aagaucuucg aggaccggaa caaggccgag uucauccgca acaucgaguc cggggccuac     1980

gagaagaugg cguacaagua ccugcccgac guggccaaga ugauccccaa gugcagcacc     2040

cagcucaacg aggccaagaa ccacuuccgg aacuccgccg acgaccugga gaucaagaag     2100

uccuucagca acccgcugaa gaucaccaag cgcaucuucg accucaacaa cauccaguac     2160

gacaagacca acgucagcaa gaagaucucc ggcgacaaca agggcaucaa gaucuuccag     2220

aaggaguacu acaagaucag cggggacuuc gacguguaca aguccgcccu gaacgacugg     2280

aucgacuucu gcaaggacuu ccugagcaag uacgacucca cgaaggacuu cgacuucagc     2340

auccuccgga agaccaagga cuacaagucc cuggacgagu ucuacgucga cguggcgaag     2400

aucaccuaca agaucagcuu cacccccgug uccgagagcu acaucgacca gaagaacaag     2460

aacggcgagc uguaccucuu cgagaucuac aaccaggacu ucgccaaggg gaagaugggc     2520

gccaagaacc ugcacacgcu guacuucgag aacgucuuca gccccgagaa caucuccaag     2580

aacuucccca ucaagcucaa cgggaacgcc gagcuguucu uccgccccaa gagcaucgag     2640

uccaagaagg agaagcggaa cuucgugcgc gagaucguga acaagaagcg cuacagcgag     2700

gacaagaucu ucuuccacug cccgaucacc cugaaccggg agaccggcuc caucuaccgc     2760

uucaacaacu acgucaacaa cuuccucagc gagaacaaca ucaacaucau cggcguggac     2820

cgcggggaga agcaccuggc cuacuacucc gugaucgaca agaacggcgu caagaucggg     2880

ggcggcagcu ucaacgagau caacaaggug gacuacgcga agaagcugga ggagcgggcc     2940

ggggagcggg agcagucccg caaggacugg caggucgugg agggcaucaa ggaccucaag     3000

aagggguaca ucagccaggu ggugcgggag cuggccgacc uggccaucaa gcacaacgcg     3060

aucaucgucc ucgaggaccu gaacaugcgc uucaagcaga uccggggcgg gaucgagaag     3120

uccaucuacc agcagcugga gaaggcccuc aucgacaagc ugagcuuccu gguggagaag     3180

ggcgagaagg accccaacca ggccggccac auccucaagg ccuaccagcu ggccgcgccc     3240

uucaccuccu ucaaggacau ggggaagcag acgggcaucg uguucuacac ccaggccagc     3300

uacaccucca agaccugccc gaacugcggg uuccgcaaga acaacaacaa guucuacuuc     3360

gagaacaaca ucggcaaggc ccaggacgcc cugaagaagc ucaagaccuu cgaguacgac     3420

agcgagaaca agugcuucgg gcuguccuac ugccugagcg acuucgccaa caaggaggag     3480

gucgagaaga acaagaacaa gaagcggaac aacgcgcccu acagcgacau cgagaagaag     3540

gacugcuucg agcucuccac gaaggacgcc gugcgcuacc gguggcacga caagaacacc     3600

gagcgcggca agaccuucuu cgagggcgag agcguguacg aggagaagga ggagaaggag     3660

aucgggcaga ccaagcgggg ccuggucaag gaguacgaca ucuccaagug ccugaucggc     3720

cucuucgaga agacggggcu ggacuacaag cagaaccugc ucgacaagau caacagcggg     3780

aaguucgacg gcaccuucua caagaaccug uucaacuacc ugaaccuccu guucgagauc     3840

cgcaacucca ucagcggcac cgagaucgac uacaucuccu gccccgagug ccaguuccac     3900

accgacaaga gcaagacgau caagaacggg gacgacaacg gcuccuacaa caucgcccgg     3960

aaggggauga ucauccugga caagaucaag caguucaaga aggagaacgg cagccucgac     4020

aagauggggu ggggcgagcu guucaucgac cuggaggagu gggacaaguu cgcccagaag     4080

aagaacaaca acaucaucga caaguga                                         4107


<210>  2313
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  2313
augagcaaga acaacgugug ggacaaguuc accaaccagu acucccugag caagacccuc       60

cgcuucgagc ugaagcccgu cggcaagacg cgggagaaga ugaacgagca gcugaaguac      120

gacaagcagc uccagaccuu ccugaaggac caggagaucg aggacgccua ccaguaccug      180

aagccguucu ucgacaagcu ccacaaggag uucaucaccg acucccugga cagcgacacc      240

acgaagaaga ucgacuucuc cgacuaccug aucgacuacc gcgacaagaa ggaccucacc      300

aagaaggagg acagccugcg gaagaacauc ggggaggugu ucgugaaggc cggcgaccgc      360

uggaagaagg agaaguaccc caaguacgag uggaagaagg gguccaacgu cgcgaacggc      420

agcgggaucc ugcucaccca ggacgugcug augcugauca aggaccucaa ccccaacgac      480

cagaagauca agaagaugau cgaggagacc uucaagggcu ucuucacgua cuucuccggc      540

uucaaccuga accgggagaa cuacuacacc accaaggugg agaaggccac cgccgucgcc      600

acgcgcaucg ugcacgacaa ccugcccaag uucugcgaca accucgugca guucgagugc      660

aucaucaaga agaagaagaa cggggagguc gaccggaucg agcgcaagag cgaguaccug      720

aacgccuacc aguaccugaa guccaagggc cggaccaccc agaucaagga cgcggagagc      780

gggaagauga ucgaggccga ggugaucaac gagaacaugu ucuccaucag cuacuucucc      840

gccugccuca gccaguccgg caucgaggag uacaaccgca ccaucgggca cuacaaccug      900

cugaucaacc ucuacaacca ggccaaggag ggcgaggaga aggaccugaa gaaguccgag      960

aagaaguuca agcggcugcc gaucuucaag acgcucuaca agcagaucgg cugcggggag     1020

aaggagcccc uguucuucag ccugacccac gacaccaagc uccaggugca ggagaacaag     1080

gagaacuaca agaagcccua cagcgucgag cagauccugg agcaggcgaa gcuggccggc     1140

gacaaguacu ucaagggcaa gagcaacgac gggauccuca acaccauccc cgaguucucc     1200

gacuacaucc ugcagaagga gagcuacggc gggguguacu gguccaaggu ggccaucaac     1260

acgaucagca accgcuacuu cgagaacugg cacacccuga aggacgcccu caucgacgcc     1320

aaggucuucg agaagggcaa gaagggggac gaggaggacg ugaagauccc ggaguccauc     1380

gagcugcagg gcuucuucga cgugcuggac acccaggagg gguggaaguc cgagggcauc     1440

uucuucaaga agucccucac cgaggagcug aagaacaagg aggcggacac gaagaacaag     1500

aagcggcgcg agaucgucaa ggaggccaag cggcccagcc aggcccugcu cgcccugcug     1560

uucgacgaca uccaggagca cgccaaccuc uuccugaacc agagcgacga gauccugaag     1620

cucgagaagu acagcgagaa ggaguccaag gagaagauca agcaguggau ggacagcgcg     1680

cuggccguga accgcauccu gaaguacuuc uucgugaagg aguccaaggc caagggcgag     1740

cagaucgacc ccaucaucag cgaggcccug aagguccucu ucaacuccga ggacgccacc     1800

ugguucaagu gguacgacgc gcugcggaac uaccugacca agaagcccca ggacgacgug     1860

aaggagaaca agcucaagcu gaacuucgag aacagcaccc uggccggggg cugggacauc     1920

aacaaggagu ccgacaacua cugcgugauc cuccaggacc agaaggggaa gcagcaccug     1980

ggcaucaucg ccaagaagga gaagcagcgc ggguacaaca agaucuucga gcggacgccg     2040

gagaaccccc uguacaagau cgacagcggc gaggucuggc agaagaugga guacaagcag     2100

aucgccgugc ccaccggcau cgggggcuuc guccgcaagu gcuucaacac cgcccagcag     2160

uacgggugga agugccccga caacugccuc aacuccgagg gcaagaucau caucaagaac     2220

gacgaggcga aggagaaccu ggaggccauc aucgacugcu acaaggacuu cuucaccaag     2280

uacgagaagg acggguucaa guacaagaac uucaacuuca acuucaagga gagcgaggag     2340

uacgaggagc ugaacagcuu cuucuccgac guggagcggc agggcuacaa gcucggcuuc     2400

aucacgguga acaagaccau caucgacaag ugggucgagg acgggaccau cuaccuguuc     2460

gagaucaaga accaggacac caacgacggc aagaaggacg ggcacaagaa caaccugcac     2520

acgaucuacu ggaaggcccu cuucgaggac gugggcaaca agccgaagcu gaacggcggg     2580

gccgagcugu ucuaccgcaa ggcgcuccug aaggacaagc aggagaagau cgaggacaac     2640

uacggcaagg agaucaucaa gaacuuccgc uucagcaagg agaaguuccu guuccacugc     2700

cccaucaagc ucaacuacaa ggccaagucc uacagccacc ccaaguacgc ccugcccgag     2760

aucaacaagc agaccaacga guucuucauc accaucuccg acaucugcuu ccuggggauc     2820

gaccggggcg agaagcaccu cgccuacuac agccugguga accagaagga ggagaucauc     2880

gaccagggga cccugaacau gccguucguc gacaaggacg gcaagccccg cuccaucaag     2940

aaggagaagu uccgguucga cgccaaggug gacaaguggg ugagcgagga gauggacugc     3000

uggaacuaca acgaccuccu ggacgcgaug gccuccaacc gcgacauggc ccggaagaac     3060

uggcagacca ucggcacgau caaggagcug aagaacgggu acaucagcca ggucgugcgc     3120

aagaucgugg accucgccau cgacaagaac cggggcaucc ccuccuucau cguccuggag     3180

aaccugaacg ccggguucau gcgcggccgg cagaagaucg agaaguccgu guaccagaag     3240

cucgagcugg cgcuggccaa gaagcucaac uucguggucg acaagaccgu gcagaacggg     3300

gagaucggca gcgugaccaa cgcccugcag cugacgcccc cggucaccaa cuuccagaac     3360

aucgagaacc gcaagcaggu cggcaucaug cucuacaccc gggccgacua caccagccag     3420

accgaccccg ugacggggug gcgcaagucc aucuaccuga agaagggcag cgaggaguac     3480

aucaaguccc agaucaugga cagcuucgac gacuucgggu ucgacggcaa gaacuacuac     3540

uucaaguaca ccguggaccu gaucgacccc aucaagaaga ccagcgagaa gaaguucaag     3600

aagcagugga cccucuacuc cgggaucaac ggcaagagcc uggaccgguu ccgcggcgag     3660

cgguccagcg acaagaacgu guggaucaag acgcugcagg acaucaagac cacccucgac     3720

gccguguuca ucggguucga ccugcagcac ucccugcgca gccagaucau caacgacggc     3780

aucaugccca agaagaucga gaacucccag uucaccgggu gggagagccu ccgguucgcg     3840

aucgagauga uccagcagau ccgcaacauc ggcgucgaga agggggacga ggacuucauc     3900

cuguccccgg ugcgggacaa gaacgagaac cacuucgaca gccgcaaggc caaggagaac     3960

gagcccaacu ccggcgacgc caacggcgcc uacaacaucg cgcgcaaggg gaucaucaug     4020

aacgagcaca ucaagaagga ccugaucccc uucauccagg acaaggagug ggacgccugg     4080

cucgccggcc gggaggugug ggacaagugg cugaucgaga acaaggacag ccugaucuac     4140

aagaaguga                                                             4149


<210>  2314
<211>  4206
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2314
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc ggacaagaag       60

uacagcaucg gccuggacau cgggaccaac uccgugggcu gggccgugau caccgacgag      120

uacaaggucc ccagcaagaa guucaaggug cuggggaaca cggaccggca cuccaucaag      180

aagaaccuga ucggcgcccu gcuguucgac agcggcgaga ccgccgaggc cacccgccug      240

aagcggaccg cgcgccggcg cuacacgcgg cgcaagaacc ggaucugcua ccugcaggag      300

aucuucucca acgagauggc caagguggac gacagcuucu uccaccgccu cgaggagucc      360

uuccuggucg aggaggacaa gaagcacgag cggcacccca ucuucgggaa caucguggac      420

gagguggccu accacgagaa guaccccacc aucuaccacc uccgcaagaa gcucgucgac      480

agcaccgaca aggccgaccu gcggcugauc uaccucgcgc uggcccacau gaucaaguuc      540

cgcggccacu uccugaucga gggggaccuc aacccggaca acuccgacgu ggacaagcug      600

uucauccagc uggugcagac cuacaaccag cuguucgagg agaaccccau caacgccagc      660

ggcgucgacg ccaaggccau ccuguccgcg cggcugagca agucccgccg gcucgagaac      720

cugaucgccc agcugcccgg ggagaagaag aacggccucu ucggcaaccu gaucgcccug      780

agccucgggc ugacgcccaa cuucaagucc aacuucgacc uggccgagga cgccaagcuc      840

cagcugagca aggacaccua cgacgacgac cuggacaacc uccuggcgca gaucggcgac      900

caguacgccg accuguuccu cgccgccaag aaccuguccg acgccauccu gcucagcgac      960

auccugcgcg ugaacaccga gaucaccaag gcgccgcugu ccgccagcau gaucaagcgg     1020

uacgacgagc accaccagga ccucacgcug cugaaggccc ucgugcgcca gcagcugccc     1080

gagaaguaca aggagaucuu cuucgaccag agcaagaacg gguacgccgg cuacaucgac     1140

ggcggggccu cccaggagga guucuacaag uucaucaagc ccauccugga gaagauggac     1200

ggcaccgagg agcuccuggu caagcugaac cgggaggacc ugcuccgcaa gcagcgcacc     1260

uucgacaacg ggagcauccc ccaccagauc caccugggcg agcugcacgc gauccuccgg     1320

cgccaggagg acuucuaccc guuccugaag gacaaccggg agaagaucga gaagauccug     1380

accuuccgca uccccuacua cguggggccc cucgcccggg gcaacucccg cuucgccugg     1440

augacgcgca agagcgagga gaccaucacc cccuggaacu ucgaggaggu ggucgacaag     1500

ggcgccuccg cgcagagcuu caucgagcgg augaccaacu ucgacaagaa ccugccgaac     1560

gagaaggugc ugcccaagca cucccuccug uacgaguacu ucacggugua caacgagcug     1620

accaagguca aguacgugac cgaggggaug cggaagcccg ccuuccucag cggcgagcag     1680

aagaaggcca ucguggaccu gcuguucaag accaaccgca aggucacggu gaagcagcuc     1740

aaggaggacu acuucaagaa gaucgagugc uucgacuccg ucgagaucag cgggguggag     1800

gaccgguuca acgccucccu gggcaccuac cacgaccugc ucaagaucau caaggacaag     1860

gacuuccugg acaacgagga gaacgaggac auccuggagg acaucgugcu cacccugacc     1920

cuguucgagg accgcgagau gaucgaggag cggcucaaga cguacgccca ccuguucgac     1980

gacaagguca ugaagcagcu gaagcgccgg cgcuacaccg gguggggccg gcucagccgc     2040

aagcugauca acggcauccg ggacaagcag uccgggaaga ccauccugga cuuccucaag     2100

agcgacggcu ucgcgaaccg caacuucaug cagcugaucc acgacgacuc ccugaccuuc     2160

aaggaggaca uccagaaggc ccaggugagc gggcagggcg acucccucca cgagcacauc     2220

gccaaccugg ccgggagccc cgccaucaag aagggcaucc ugcagacggu gaaggucgug     2280

gacgagcucg ugaaggucau gggccgccac aagccggaga acaucgugau cgagauggcg     2340

cgggagaacc agaccaccca gaaggggcag aagaacuccc gcgagcggau gaagcgcauc     2400

gaggagggca ucaaggagcu ggggagccag auccugaagg agcaccccgu ggagaacacc     2460

cagcuccaga acgagaagcu guaccuguac uaccuccaga acggccggga cauguacguc     2520

gaccaggagc uggacaucaa ccgccugagc gacuacgacg uggaccacau cgugccccag     2580

uccuuccuca aggacgacag caucgacaac aagguccuga cgcgguccga caagaaccgc     2640

ggcaagagcg acaacgugcc cuccgaggag guggucaaga agaugaagaa cuacuggcgg     2700

cagcugcuca acgccaagcu gaucacccag cgcaaguucg acaaccugac caaggccgag     2760

cgcgggggcc ucagcgagcu ggacaaggcc ggguucauca agcggcagcu gguggagacc     2820

cggcagauca ccaagcacgu ggcccagauc cucgacuccc gcaugaacac gaaguacgac     2880

gagaacgaca agcugauccg ggaggucaag gugaucaccc ucaagagcaa gcucgugucc     2940

gacuuccgca aggacuucca guucuacaag guccgggaga ucaacaacua ccaccacgcg     3000

cacgacgccu accugaacgc cguggugggc accgcccuga ucaagaagua cccgaagcuc     3060

gagagcgagu ucgucuacgg ggacuacaag guguacgacg ugcgcaagau gaucgcgaag     3120

uccgagcagg agaucggcaa ggccaccgcc aaguacuucu ucuacagcaa caucaugaac     3180

uucuucaaga cggagaucac ccuggccaac ggcgagaucc ggaagcgccc ccugaucgag     3240

accaacgggg agaccggcga gaucgucugg gacaaggggc gggacuucgc cacggugcgc     3300

aaggugcucu ccaugcccca ggucaacauc gugaagaaga ccgaggugca gaccggcggg     3360

uucagcaagg aguccauccu gcccaagcgc aacagcgaca agcugaucgc gcggaagaag     3420

gacugggacc cgaagaagua cggcggcuuc gacuccccca ccgucgccua cagcgugcuc     3480

guggucgcca agguggagaa gggcaagagc aagaagcuga aguccgugaa ggagcugcuc     3540

gggaucacga ucauggagcg cagcuccuuc gagaagaacc ccaucgacuu ccuggaggcc     3600

aagggguaca aggaggucaa gaaggaccug aucaucaagc ucccgaagua cagccuguuc     3660

gagcuggaga acggccggaa gcgcaugcuc gccuccgcgg gggagcugca gaagggcaac     3720

gagcuggccc uccccagcaa guacgugaac uuccuguacc uggccuccca cuacgagaag     3780

cucaagggca gccccgagga caacgagcag aagcagcugu ucguggagca gcacaagcac     3840

uaccuggacg agaucaucga gcagaucucc gaguucagca agcgggucau ccucgccgac     3900

gccaaccugg acaaggugcu guccgcguac aacaagcacc gcgacaagcc cauccgggag     3960

caggccgaga acaucaucca ccucuucacc cugaccaacc ugggggcccc ggccgcguuc     4020

aaguacuucg acaccacgau cgaccgcaag cgguacacca gcaccaagga ggugcucgac     4080

gccacccuga uccaccaguc caucacgggc cuguacgaga cccgcaucga ccucagccag     4140

cucgggggcg acaagcggcc cgccgccacc aagaaggccg ggcaggcgaa gaagaagaag     4200

uaauga                                                                4206


<210>  2315
<211>  4989
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2315
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gaacuucaag       60

auccugccca ucgccaucga ccucggcgug aagaacaccg ggguguucag cgccuucuac      120

cagaagggca ccucccugga gcggcuggac aacaagaacg ggaaggucua cgagcucagc      180

aaggacuccu acacgcugcu gaugaacaac cgcaccgccc ggcgccacca gcggcgcggc      240

aucgaccgga agcagcucgu gaagcgccug uucaagcuga ucuggaccga gcagcucaac      300

cuggaguggg acaaggacac ccagcaggcc aucagcuucc uguucaaccg gcgcggcuuc      360

uccuucauca cggacgggua cagccccgag uaccucaaca ucgugcccga gcaggucaag      420

gcgauccuga uggacaucuu cgacgacuac aacggcgagg acgaccugga cuccuaccuc      480

aagcuggcca ccgagcagga gagcaagauc uccgagaucu acaacaagcu gaugcagaag      540

auccucgagu ucaagcugau gaagcugugc accgacauca aggacgacaa ggugagcacc      600

aagacgcuca aggagaucac cuccuacgag uucgagcugc uggccgacua ccucgccaac      660

uacagcgagu cccugaagac ccagaaguuc agcuacaccg acaagcaggg gaaccugaag      720

gagcucuccu acuaccacca cgacaaguac aacauccagg aguuccugaa gcggcacgcg      780

acgaucaacg accgcauccu ggacacccuc cugaccgacg accuggacau cuggaacuuc      840

aacuucgaga aguucgacuu cgacaagaac gaggagaagc uccagaacca ggaggacaag      900

gaccacaucc aggcccaccu ccaccacuuc guguucgccg ucaacaagau caagagcgag      960

auggccuccg gcgggcggca ccgcagccag uacuuccagg agaucaccaa cgugcuggac     1020

gagaacaacc accaggaggg cuaccugaag aacuucugcg agaaccugca caacaagaag     1080

uacuccaacc ugagcgugaa gaaccucguc aaccugaucg gcaaccugag caaccucgag     1140

cugaagccgc ugcggaagua cuucaacgac aagauccacg ccaaggcgga ccacugggac     1200

gagcagaagu ucacggagac cuacugccac uggauccucg gggaguggcg cgugggcgug     1260

aaggaccagg acaagaagga cggggccaag uacuccuaca aggaccugug caacgagcug     1320

aagcagaagg ucaccaaggc cggccucgug gacuuccugc uggagcugga ccccugccgg     1380

accaucccgc ccuaccucga caacaacaac cgcaagccgc ccaagugcca gagccugauc     1440

cugaacccca aguuccucga caaccaguac cccaacuggc agcaguaccu gcaggagcug     1500

aagaagcucc aguccaucca gaacuaccug gacagcuucg agacggaccu gaaggugcuc     1560

aaguccagca aggaccagcc cuacuucguc gaguacaagu ccagcaacca gcagaucgcc     1620

uccggccagc gggacuacaa ggaccuggac gcccgcaucc ugcaguucau cuucgaccgg     1680

gugaaggcga gcgacgagcu ccugcugaac gagaucuacu uccaggccaa gaagcucaag     1740

cagaaggccu ccagcgagcu ggagaagcug gaguccagca agaagcucga cgaggugauc     1800

gccaacuccc agcugagcca gauccugaag ucccagcaca ccaacgggau cuucgagcag     1860

ggcaccuucc uccaccuggu cugcaaguac uacaagcagc gccagcgcgc ccgggacagc     1920

cgccuguaca ucaugcccga guaccgguac gacaagaagc uccacaagua caacaacacc     1980

gggcgcuucg acgacgacaa ccagcugcug acguacugca accacaagcc ccggcagaag     2040

cgcuaccagc uccugaacga ccuggcgggc gugcuccagg ugucccccaa cuuccugaag     2100

gacaagaucg ggagcgacga cgaccuguuc aucagcaagu ggcucgucga gcacauccgg     2160

ggcuucaaga aggccugcga ggacucccug aagauccaga aggacaaccg cggccugcuc     2220

aaccacaaga ucaacaucgc ccggaacacc aaggggaagu gcgagaagga gaucuucaac     2280

cugaucugca agaucgaggg cagcgaggac aagaagggga acuacaagca cggccuggcc     2340

uacgagcucg gggugcugcu guucggcgag ccgaacgagg ccuccaagcc cgaguucgac     2400

cgcaagauca agaaguucaa cagcaucuac uccuucgcgc agauccagca gaucgccuuc     2460

gccgagcgga agggcaacgc caacacgugc gcggucugca gcgccgacaa cgcccaccgc     2520

augcagcaga ucaagaucac cgagcccgug gaggacaaca aggacaagau cauccucucc     2580

gccaaggccc agcggcugcc cgcgaucccg acccgcaucg uggacggggc cgucaagaag     2640

auggccacca uccuggccaa gaacaucgug gacgacaacu ggcagaacau caagcagguc     2700

cucagcgcca agcaccagcu gcacaucccc aucaucaccg aguccaacgc guucgaguuc     2760

gagcccgccc uggccgacgu gaagggcaag agccucaagg accggcgcaa gaaggcccug     2820

gagcggaucu cccccgagaa caucuucaag gacaagaaca accgcaucaa ggaguucgcc     2880

aaggggauca gcgcguacuc cggcgccaac cugaccgacg gggacuucga cggcgccaag     2940

gaggagcucg accacaucau cccgcggagc cacaagaagu acggcacgcu gaacgacgag     3000

gccaaccuga ucugcgugac ccgcggggac aacaagaaca agggcaaccg caucuucugc     3060

cuccgggacc uggcggacaa cuacaagcug aagcaguucg agaccaccga cgaccucgag     3120

aucgagaaga agaucgccga cacgaucugg gacgccaaca agaaggacuu caaguucggg     3180

aacuaccgcu ccuucaucaa ccugacgccc caggagcaga aggccuuccg gcacgcccug     3240

uuccucgcgg acgagaaccc caucaagcag gccgugaucc gcgccaucaa caaccggaac     3300

cgcaccuucg ucaacggcac ccagcgguac uucgccgagg ugcuggccaa caacaucuac     3360

cugcgcgcga agaaggagaa ccucaacacc gacaagauca gcuucgacua cuucggcauc     3420

cccaccaucg ggaacggccg ggggaucgcc gagauccgcc agcuguacga gaagguggac     3480

uccgacaucc aggccuacgc caagggcgac aagccgcagg ccagcuacag ccaccugauc     3540

gacgcgaugc ucgccuucug caucgccgcc gacgagcacc ggaacgacgg guccaucggc     3600

cuggagaucg acaagaacua cagccuguac ccccucgaca agaacaccgg cgaggucuuc     3660

accaaggaca ucuucuccca gaucaagauc accgacaacg aguucagcga caagaagcug     3720

gugcgcaaga aggcgaucga gggguucaac acgcaccggc agaugacccg cgacggcauc     3780

uacgccgaga acuaccugcc cauccucauc cacaaggagc ugaacgaggu gcggaagggg     3840

uacaccugga agaacuccga ggagaucaag aucuucaagg gcaagaagua cgacauccag     3900

cagcugaaca accucgucua cugccugaag uucguggaca agccgaucag caucgacauc     3960

cagaucucca cccuggagga gcuccgcaac auccugacga ccaacaacau cgccgccacc     4020

gccgaguacu acuacaucaa ccugaagacc cagaagcucc acgaguacua caucgagaac     4080

uacaacacgg cgcuggggua caagaaguac agcaaggaga uggaguuccu gcggucccuc     4140

gccuaccgca gcgagcgggu gaagaucaag uccaucgacg acgucaagca gguccuggac     4200

aaggacagca acuucaucau cggcaagauc acccugcccu ucaagaagga guggcagcgc     4260

cucuaccggg aguggcagaa caccaccauc aaggacgacu acgaguuccu gaaguccuuc     4320

uucaacguca agagcaucac gaagcugcac aagaaggugc gcaaggacuu cucccucccc     4380

aucagcacca acgagggcaa guuccuggug aagcgcaaga ccugggacaa caacuucauc     4440

uaccagaucc ugaacgacuc cgacagccgg gccgacggga ccaagcccuu caucccggcc     4500

uucgacaucu ccaagaacga gaucguggag gccaucaucg acagcuucac gagcaagaac     4560

aucuucuggc uccccaagaa caucgagcug cagaaggugg acaacaagaa caucuucgcg     4620

aucgacaccu ccaagugguu cgagguggag acccccagcg accugcgcga caucggcauc     4680

gccaccaucc aguacaagau cgacaacaac ucccggccca aggugcgcgu caagcuggac     4740

uacgugaucg acgacgacag caagaucaac uacuucauga accacucccu ccucaagagc     4800

cgguacccgg acaaggugcu ggagauccug aagcagucca cgaucaucga guucgagagc     4860

uccggguuca acaagaccau caaggagaug cugggcauga agcuggccgg gaucuacaac     4920

gagaccagca acaacaagcg ccccgccgcc accaagaagg cgggccaggc caagaagaag     4980

aaguaauga                                                             4989


<210>  2316
<211>  3261
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2316
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gaagcggaac       60

uacauccugg gccucgacau cgggaucacc agcgugggcu acgggaucau cgacuacgag      120

acccgcgacg ugaucgacgc cggcguccgg cuguucaagg aggccaacgu ggagaacaac      180

gagggccgcc gguccaagcg cggggcccgg cgccugaagc ggcgccggcg ccaccggauc      240

cagcgcguga agaagcuccu guucgacuac aaccugcuca cggaccacag cgagcugucc      300

ggcaucaacc ccuacgaggc ccgggucaag gggcugagcc agaagcucuc cgaggaggag      360

uucagcgcgg cccugcugca ccucgccaag cgccggggcg ugcacaacgu gaacgagguc      420

gaggaggaca ccgggaacga gcuguccacc aaggagcaga ucagccgcaa cuccaaggcc      480

cuggaggaga aguacguggc ggagcuccag cuggagcggc ugaagaagga cggcgaggug      540

cgcggcagca ucaaccgguu caagaccucc gacuacguca aggaggccaa gcagcuccug      600

aaggugcaga aggccuacca ccagcuggac cagagcuuca ucgacacgua caucgaccuc      660

cuggagaccc gccgcaccua cuacgagggg cccggcgagg ggucccccuu cggcuggaag      720

gacaucaagg agugguacga gaugcugaug ggccacugca ccuacuuccc ggaggagcuc      780

cggagcguga aguacgccua caacgccgac cuguacaacg cgcugaacga ccucaacaac      840

cuggucauca cgcgcgacga gaacgagaag cuggaguacu acgagaaguu ccagaucauc      900

gagaacgugu ucaagcagaa gaagaagccc acccucaagc agaucgccaa ggagauccug      960

gugaacgagg aggacaucaa gggguaccgg gucaccucca ccggcaagcc cgaguucacg     1020

aaccugaagg uguaccacga caucaaggac aucaccgccc gcaaggagau caucgagaac     1080

gccgagcucc uggaccagau cgccaagauc cugaccaucu accagagcuc cgaggacauc     1140

caggaggagc ucaccaaccu gaacagcgag cugacgcagg aggagaucga gcagaucucc     1200

aaccucaagg gguacaccgg cacccacaac cugagccuga aggcgaucaa ccucauccug     1260

gacgagcugu ggcacaccaa cgacaaccag aucgccaucu ucaaccggcu caagcuggug     1320

cccaagaagg ucgaccugag ccagcagaag gagaucccga cgacccucgu ggacgacuuc     1380

auccuguccc ccguggucaa gcgcagcuuc auccagucca ucaaggugau caacgccauc     1440

aucaagaagu acgggcugcc caacgacauc aucaucgagc uggcccggga gaagaacagc     1500

aaggacgccc agaagaugau caacgagaug cagaagcgca accggcagac caacgagcgc     1560

aucgaggaga ucauccggac cacgggcaag gagaacgcga aguaccucau cgagaagauc     1620

aagcugcacg acaugcagga gggcaagugc cuguacuccc ucgaggccau cccccuggag     1680

gaccugcuca acaacccguu caacuacgag gucgaccaca ucaucccccg cagcgugucc     1740

uucgacaaca gcuucaacaa caaggugcug gucaagcagg aggagaacuc caagaagggg     1800

aaccggaccc ccuuccagua ccugagcucc agcgacucca agaucagcua cgagaccuuc     1860

aagaagcaca uccucaaccu ggccaagggc aaggggcgca ucuccaagac caagaaggag     1920

uaccugcucg aggagcggga caucaaccgc uucagcgugc agaaggacuu caucaaccgg     1980

aaccuggugg acacgcgcua cgccacccgg ggccugauga accuccugcg cuccuacuuc     2040

cgcgucaaca accuggacgu gaaggucaag agcaucaacg ggggcuucac cuccuuccuc     2100

cggcgcaagu ggaaguucaa gaaggagcgg aacaagggcu acaagcacca cgccgaggac     2160

gcgcugauca ucgccaacgc cgacuucauc uucaaggagu ggaagaagcu ggacaaggcc     2220

aagaagguga uggagaacca gauguucgag gagaagcagg cggagagcau gcccgagauc     2280

gagaccgagc aggaguacaa ggagaucuuc aucacgccgc accagaucaa gcacaucaag     2340

gacuucaagg acuacaagua cagccaccgc guggacaaga agcccaaccg ggagcucauc     2400

aacgacaccc uguacuccac ccgcaaggac gacaagggga acacccugau cgugaacaac     2460

cucaacggcc uguacgacaa ggacaacgac aagcugaaga agcucaucaa caagagcccc     2520

gagaagcugc ugauguacca ccacgacccc cagacguacc agaagcucaa gcugaucaug     2580

gagcaguacg gggacgagaa gaacccgcug uacaaguacu acgaggagac cggcaacuac     2640

cucaccaagu acuccaagaa ggacaacggg cccgucauca agaagaucaa guacuacggc     2700

aacaagcuga acgcccaccu ggacaucacc gacgacuacc ccaacagccg gaacaaggug     2760

gugaagcucu cccugaagcc cuaccgcuuc gacgucuacc uggacaacgg cguguacaag     2820

uucgugaccg ucaagaaccu cgacgugauc aagaaggaga acuacuacga ggugaacagc     2880

aagugcuacg aggaggccaa gaagcugaag aagaucucca accaggccga guucaucgcc     2940

agcuucuaca acaacgaccu gaucaagauc aacggggagc ucuaccgggu caucggcgug     3000

aacaacgacc ugcugaaccg caucgaggug aacaugaucg acaucacgua ccgggaguac     3060

cucgagaaca ugaacgacaa gcgcccgccc cggaucauca agaccaucgc guccaagacc     3120

cagagcauca agaaguacuc caccgacauc cuggggaacc uguacgaggu caagagcaag     3180

aagcaccccc agaucaucaa gaagggcaag cgccccgccg ccacgaagaa ggccggccag     3240

gccaagaaga agaaguaaug a                                               3261


<210>  2317
<211>  4329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2317
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gcuguucaac       60

aagugcauca ucaucagcau caaccucgac uucuccaaca aggagaagug caugaccaag      120

cccuacagca ucggccugga caucgggacc aacuccgugg gcugggccgu gaucacggac      180

aacuacaagg uccccagcaa gaagaugaag gugcugggga acaccuccaa gaaguacauc      240

aagaagaacc uccugggcgu gcugcuguuc gacagcggca ucaccgccga ggggcggcgc      300

cugaagcgga ccgcccgccg gcgcuacacg cggcgccgga accgcauccu guaccuccag      360

gagaucuucu ccaccgagau ggccacccug gacgacgcgu ucuuccagcg gcuggacgac      420

agcuuccucg uccccgacga caagcgcgac uccaaguacc cgaucuucgg caaccuggug      480

gaggagaagg uguaccacga cgaguucccc accaucuacc accuccggaa guaccucgcc      540

gacagcacga agaaggccga ccugcgccug gucuaccucg cccuggcgca caugaucaag      600

uaccgggggc acuuccugau cgagggcgag uucaacucca agaacaacga cauccagaag      660

aacuuccagg acuuccucga caccuacaac gccaucuucg agagcgaccu gucccuggag      720

aacagcaagc agcucgagga gaucgugaag gacaagaucu ccaagcugga gaagaaggac      780

cgcauccuga agcuguuccc cggggagaag aacagcggca ucuucuccga guuccugaag      840

cugaucgugg gcaaccaggc cgacuuccgg aagugcuuca accucgacga gaaggccagc      900

cugcacuucu ccaaggagag cuacgacgag gaccuggaga cccuccuggg guacaucggc      960

gacgacuaca gcgacgucuu ccugaaggcc aagaagcucu acgacgcgau ccugcugucc     1020

ggguuccuca ccgugacgga caacgagacc gaggcgcccc ugagcuccgc caugaucaag     1080

cgcuacaacg agcacaagga ggaccuggcc cuccugaagg aguacauccg gaacaucagc     1140

cugaagaccu acaacgaggu guucaaggac gacaccaaga acggcuacgc cggcuacauc     1200

gacgggaaga cgaaccagga ggacuucuac gucuaccuca agaaccugcu ggccgaguuc     1260

gagggcgccg acuacuuccu cgagaagauc gaccgcgagg acuuccugcg gaagcagcgc     1320

accuucgaca acggguccau cccguaccag auccaccucc aggagaugcg cgccauccug     1380

gacaagcagg ccaaguucua ccccuuccuc gccaagaaca aggagcggau cgagaagauc     1440

cugaccuucc gcauccccua cuacgugggc ccccuggcgc gggggaacag cgacuucgcc     1500

ugguccaucc gcaagcggaa cgagaagauc accccgugga acuucgagga cgugaucgac     1560

aaggagagcu ccgccgaggc cuucaucaac cgcaugacga gcuucgaccu cuaccugccc     1620

gaggagaagg uccugcccaa gcacucccuc cuguacgaga ccuucaacgu guacaacgag     1680

cugaccaagg ugcgguucau cgccgagagc augcgcgacu accaguuccu cgacuccaag     1740

cagaagaagg acaucguccg gcuguacuuc aaggacaagc gcaaggugac cgacaaggac     1800

aucaucgagu accugcacgc gaucuacggc uacgacggca ucgagcucaa ggggaucgag     1860

aagcaguuca acagcucccu gagcacguac cacgaccugc ucaacaucau caacgacaag     1920

gaguuccugg acgacuccag caacgaggcc aucaucgagg agaucaucca cacccugacc     1980

aucuucgagg accgggagau gaucaagcag cgccucucca aguucgagaa caucuucgac     2040

aagagcgugc ugaagaagcu gagccggcgc cacuacaccg gcugggggaa gcucuccgcc     2100

aagcugauca acggcauccg ggacgagaag uccgggaaca cgauccugga cuaccucauc     2160

gacgacggca ucagcaaccg caacuucaug cagcugaucc acgacgacgc ccugagcuuc     2220

aagaagaaga uccagaaggc gcagaucauc ggcgacgagg acaaggggaa caucaaggag     2280

gucgugaagu cccuccccgg cagcccggcc aucaagaagg ggauccugca guccaucaag     2340

aucgucgacg agcuggugaa ggugaugggc gggcggaagc ccgagagcau cgucguggag     2400

auggcccgcg agaaccagua caccaaccag ggcaagucca acagccagca gcggcucaag     2460

cgccuggaga agucccugaa ggagcucggc agcaagaucc ugaaggagaa cauccccgcc     2520

aagcugucca agaucgacaa caacgcccuc cagaacgacc gccuguaccu guacuaccuc     2580

cagaacggga aggacaugua caccggcgac gaccuggaca ucgaccggcu gagcaacuac     2640

gacaucgacc acaucauccc ccaggcguuc cucaaggaca acuccaucga caacaaggug     2700

cuggucagcu ccgccagcaa ccgcgggaag uccgacgacu ucccgagccu ggagguggug     2760

aagaagcgga agaccuucug guaccagcuc cucaagagca agcugaucuc ccagcgcaag     2820

uucgacaacc ugacgaaggc cgagcggggc ggccugcugc ccgaggacaa ggccggguuc     2880

auccagcgcc agcucgucga gacccggcag aucaccaagc acguggcccg ccugcuggac     2940

gagaaguuca acaacaagaa ggacgagaac aaccgggcgg ugcgcaccgu caagaucauc     3000

acgcucaaga gcacccuggu gucccaguuc cggaaggacu ucgagcugua caaggugcgc     3060

gagaucaacg acuuccacca cgcccacgac gccuaccuca acgccgucau cgccagcgcg     3120

cugcugaaga aguaccccaa gcucgagccc gaguucgugu acggcgacua cccgaaguac     3180

aacuccuucc gggagcgcaa gagcgccacc gagaaggugu acuucuacuc caacaucaug     3240

aacaucuuca agaaguccau cagccuggcc gacgggcggg ucaucgagcg cccccugauc     3300

gaggugaacg aggagaccgg cgagagcgug uggaacaagg aguccgaccu cgccaccguc     3360

cggcgcgugc ugagcuaccc ccaggugaac gucgugaaga agguggagga gcagaaccac     3420

gggcuggacc ggggcaagcc caagggccuc uucaacgcga accuguccag caagccgaag     3480

cccaacucca acgagaaccu ggucggggcc aaggaguacc ucgaccccaa gaaguacggc     3540

ggguacgccg gcaucagcaa cuccuucgcc gugcugguga aggggacgau cgagaagggc     3600

gccaagaaga agaucaccaa cguccuggag uuccagggca ucagcauccu cgaccgcauc     3660

aacuaccgga aggacaagcu gaacuuccug cucgagaagg gguacaagga caucgagcug     3720

aucaucgagc ugccgaagua cagccucuuc gagcuguccg acggcagccg ccgcaugcug     3780

gcguccaucc ucagcaccaa caacaagcgc ggggagaucc acaagggcaa ccagaucuuc     3840

cugucccaga aguucgugaa gcugcucuac cacgccaagc ggaucagcaa caccaucaac     3900

gagaaccacc ggaaguacgu ggagaaccac aagaaggagu ucgaggagcu guucuacuac     3960

auccuggagu ucaacgagaa cuacgucggg gccaagaaga acggcaagcu ccugaacucc     4020

gccuuccaga gcuggcagaa ccacuccauc gacgagcugu gcagcuccuu caucggcccc     4080

acggggagcg agcgcaaggg ccucuucgag cugaccuccc gggggagcgc cgcggacuuc     4140

gaguuccugg gcgugaagau cccccgcuac cgggacuaca cccccuccag ccuccugaag     4200

gacgccaccc ugauccacca guccgugacg gggcuguacg agacccgcau cgaccucgcc     4260

aagcugggcg agggcaagcg gccggccgcc accaagaagg cggggcaggc caagaagaag     4320

aaguaauga                                                             4329


<210>  2318
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2318
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gugguacgcc       60

agccugaugu ccgcccacca ccuccgggug ggcaucgacg uggggaccca cagcgucggc      120

cuggccaccc ugcgcgugga cgaccacggg acgcccaucg agcuccuguc cgcccugagc      180

cacauccacg acuccggcgu gggcaaggag gggaagaagg accacgacac ccggaagaag      240

cucagcggca ucgcgcgccg ggcccgccgg cugcugcacc accgccggac ccagcuccag      300

cagcuggacg agguccugcg cgaccucggg uuccccaucc ccaccccggg cgaguuccug      360

gaccugaacg agcagacgga ccccuaccgg guguggcgcg ugcgggcccg ccucgucgag      420

gagaagcugc ccgaggagcu gcgggggccc gccaucucca uggcggugcg ccacaucgcc      480

cggcaccgcg gcuggcggaa cccguacagc aagguggagu cccuccugag ccccgccgag      540

gagucccccu ucaugaaggc ccugcgcgag cggauccucg ccaccaccgg cgagguccug      600

gacgacggga ucacccccgg ccaggcgaug gcccaggugg cccugacgca caacaucagc      660

augcgcgggc cggagggcau ccucggcaag cugcaccagu ccgacaacgc caacgagauc      720

cgcaagaucu gcgcccggca gggggugagc cccgacgucu gcaagcagcu gcuccgcgcg      780

guguucaagg ccgacagccc ccggggcucc gccguguccc gcgucgcccc cgacccgcug      840

cccgggcagg gcagcuuccg gcgcgccccc aagugcgacc ccgaguucca gcgguuccgc      900

aucaucucca ucguggcgaa ccugcggauc agcgagacca agggggagaa ccgcccgcuc      960

accgccgacg agcggcgcca cguggucacc uuccugacgg aggacagcca ggccgaccug     1020

accugggugg acguggccga gaagcucggc guccaccggc gcgaccugcg gggcaccgcc     1080

gugcacaccg acgacgggga gcgcuccgcg gcccggccgc ccaucgacgc cacggaccgc     1140

aucaugcggc agaccaagau cagcucccug aagaccuggu gggaggaggc cgacagcgag     1200

cagcgcggcg cgaugauccg cuaccucuac gaggacccca ccgacuccga gugcgccgag     1260

aucaucgccg agcugcccga ggaggaccag gccaagcugg acagccucca ccuccccgcc     1320

gggcgggcgg ccuacucccg cgagagccug acggcccugu ccgaccacau gcuggccacc     1380

accgacgacc ugcacgaggc ccggaagcgc cucuucggcg ucgacgacag cugggcgccg     1440

cccgccgagg ccaucaacgc ccccgugggg aaccccuccg uggaccggac ccugaagauc     1500

gucggccgcu accugagcgc cguggagucc auguggggca cgcccgaggu gauccacguc     1560

gagcacgugc gggacggguu caccagcgag cgcauggcgg acgagcggga caaggccaac     1620

cgccggcgcu acaacgacaa ccaggaggcc augaagaaga uccagcggga cuacggcaag     1680

gagggguaca ucucccgcgg cgacaucgug cggcucgacg cccuggagcu gcaggggugc     1740

gcgugccugu acugcggcac caccaucggc uaccacacgu gccagcucga ccacaucguc     1800

ccgcaggccg ggcccggcag caacaaccgc cgggggaacc ugguggccgu gugcgagcgc     1860

ugcaaccggu ccaagagcaa cacccccuuc gccgucuggg cccagaagug cggcaucccc     1920

cacgugggcg ugaaggaggc gaucgggcgc guccggggcu ggcgcaagca gaccccgaac     1980

accuccagcg aggaccugac gcgccucaag aaggagguga ucgcccggcu gcgccggacc     2040

caggaggacc ccgagaucga cgagcgcagc auggaguccg uggccuggau ggccaacgag     2100

cugcaccacc ggaucgccgc ggccuacccc gagaccaccg ucauggugua ccgcgggagc     2160

aucacggccg ccgcccggaa ggcggccggc aucgacuccc gcaucaaccu caucggggag     2220

aagggccgga aggaccgcau cgaccggcgc caccacgccg uggacgccag cgucguggcg     2280

cugauggagg ccuccguggc caagacccug gccgagcgga gcucccuccg cggcgagcag     2340

cggcugaccg ggaaggagca gaccuggaag caguacaccg gcagcacggu cggggcccgc     2400

gagcacuucg agauguggcg gggccacaug cugcaccuca ccgagcuguu caacgagcgc     2460

cuggcggagg acaaggugua cgugacccag aacauccggc uccgccuguc cgacgggaac     2520

gcccacaccg ucaaccccag caagcuggug ucccaccgcc ucggcgacgg ccugacggug     2580

cagcagaucg accgggccug caccccggcc cuguggugcg cccucacccg cgagaaggac     2640

uucgacgaga agaacgggcu gcccgcgcgg gaggaccgcg ccauccgggu ccacggccac     2700

gagaucaaga gcuccgacua cauccaggug uucagcaagc gcaagaagac cgacuccgac     2760

cgggacgaga cgcccuucgg ggccaucgcc gugcgcggcg gguucgucga gaucggcccc     2820

agcauccacc acgcccggau cuaccgcgug gagggcaaga agccggugua cgcgaugcug     2880

cgggucuuca cccacgaccu ccugucccag cgccacgggg accuguucag cgccgugauc     2940

ccgccccaga gcaucuccau gcggugcgcc gagcccaagc uccgcaaggc caucaccacc     3000

ggcaacgcca cguaccuggg gugggugguc gugggcgacg agcuggagau caacguggac     3060

agcuucacca aguacgcgau cgggcgguuc cucgaggacu uccccaacac cacccgcugg     3120

cggaucugcg gcuacgacac gaacuccaag cugacccuga agcccaucgu ccucgccgcc     3180

gagggccugg agaaccccag cuccgccgug aacgagaucg uggagcugaa gggguggcgc     3240

gucgcgauca acgugcucac caaggugcac cccaccgucg ugcggcgcga cgcccugggc     3300

cggccgcgcu acagcucccg cagcaaccug cccacguccu ggaccaucga gaagcggccc     3360

gccgccacca agaaggccgg gcaggcgaag aagaagaagu aauga                     3405


<210>  2319
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2319
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gaccaagccc       60

uacagcaucg gccuggacau cgggaccaac uccgugggcu gggccgugac gaccgacaac      120

uacaaggucc ccagcaagaa gaugaaggug cucgggaaca ccuccaagaa guacaucaag      180

aagaaccugc ugggcgugcu ccuguucgac agcggcauca ccgccgaggg gcggcgccug      240

aagcggacgg cccgccggcg cuacacccgg cgccggaacc gcauccucua ccugcaggag      300

aucuucucca ccgagauggc cacccuggac gacgcguucu uccagcggcu cgacgacagc      360

uuccuggucc ccgacgacaa gcgcgacucc aaguacccga ucuucggcaa ccugguggag      420

gagaaggccu accacgacga guuccccacg aucuaccacc uccggaagua ccuggccgac      480

agcaccaaga aggccgaccu gcgccucgug uaccuggcgc uggcccacau gaucaaguac      540

cgggggcacu uccucaucga gggcgaguuc aacuccaaga acaacgacau ccagaagaac      600

uuccaggacu uccuggacac cuacaacgcc aucuucgaga gcgaccuguc ccucgagaac      660

agcaagcagc uggaggagau cgucaaggac aagaucucca agcuggagaa gaaggaccgc      720

auccucaagc uguuccccgg ggagaagaac agcggcaucu ucuccgaguu ccugaagcuc      780

aucgugggca accaggccga cuuccggaag ugcuucaacc uggacgagaa ggccagccug      840

cacuucucca aggagagcua cgacgaggac cucgagaccc ugcuggggua caucggcgac      900

gacuacuccg acguguuccu caaggcgaag aagcuguacg acgccauccu gcucagcggg      960

uuccugacgg ucaccgacaa cgagaccgag gcgccccuga gcuccgccau gaucaagcgc     1020

uacaacgagc acaaggagga ccucgcccug cugaaggagu acauccggaa caucagccuc     1080

aagaccuaca acgagguguu caaggacgac acgaagaacg gcuacgccgg cuacaucgac     1140

gggaagacca accaggagga cuucuacgug uaccugaaga agcugcucgc cgaguucgag     1200

ggcgccgacu acuuccugga gaagaucgac cgcgaggacu uccugcggaa gcagcgcacc     1260

uucgacaacg gguccauccc guaccagauc caccuccagg agaugcgcgc cauccuggac     1320

aagcaggcca aguucuaccc cuuccuggcg aagaacaagg agcggaucga gaagauccug     1380

accuuccgca uccccuacua cgucggcccc cucgcccggg ggaacagcga cuucgccugg     1440

uccauccgca agcggaacga gaagaucacg ccguggaacu ucgaggacgu gaucgacaag     1500

gagagcuccg ccgaggccuu caucaaccgc augaccagcu ucgaccugua ccugcccgag     1560

gagaaggugc uccccaagca cucccugcug uacgagaccu ucaacgucua caacgagcuc     1620

accaaggugc gguucaucgc ggagagcaug cgcgacuacc aguuccugga cuccaagcag     1680

aagaaggaca ucgugcggcu guacuucaag gacaagcgca aggucacgga caaggacauc     1740

aucgaguacc uccacgccau cuacggcuac gacggcaucg agcugaaggg gaucgagaag     1800

caguucaaca gcucccugag caccuaccac gaccuccuga acaucaucaa cgacaaggag     1860

uuccuggacg acuccagcaa cgaggccauc aucgaggaga ucauccacac ccucaccauc     1920

uucgaggacc gggagaugau caagcagcgc cuguccaagu ucgagaacau cuucgacaag     1980

agcgugcuga agaagcucuc ccggcgccac uacacgggcu gggggaagcu gagcgccaag     2040

cugaucaacg gcauccggga cgagaagucc gggaacacca uccucgacua ccugaucgac     2100

gacggcauca gcaaccgcaa cuucaugcag cugauccacg acgacgcgcu cagcuucaag     2160

aagaagaucc agaaggccca gaucaucggc gacgaggaca aggggaacau caaggaggug     2220

gucaaguccc ugcccggcag cccggccauc aagaagggga uccugcaguc caucaagauc     2280

guggacgagc ucgucaaggu gaugggcggg cggaagcccg agagcaucgu ggucgagaug     2340

gcccgcgaga accaguacac caaccagggc aaguccaaca gccagcagcg gcugaagcgc     2400

cuggagaagu cccucaagga gcugggcagc aagauccuga aggagaacau ccccgccaag     2460

cucuccaaga ucgacaacaa cgcgcugcag aacgaccgcc uguaccucua cuaccugcag     2520

aacgggaagg acauguacac cggcgacgac cuggacaucg accggcucag caacuacgac     2580

aucgaccaca ucauccccca ggccuuccug aaggacaacu ccaucgacaa caaggugcug     2640

gugagcuccg ccagcaaccg cgggaagucc gacgacgucc cgagccucga gguggugaag     2700

aagcggaaga cguucuggua ccagcugcuc aagagcaagc ugaucuccca gcgcaaguuc     2760

gacaaccuga ccaaggccga gcggggcggc cugagccccg aggacaaggc cggguucauc     2820

cagcgccagc ucgucgagac ccggcagauc accaagcacg uggcgcgccu gcuggacgag     2880

aaguucaaca acaagaagga cgagaacaac cgggccgugc gcacggucaa gaucaucacc     2940

cucaagucca cccuggugag ccaguuccgg aaggacuucg agcuguacaa ggugcgcgag     3000

aucaacgacu uccaccacgc ccacgacgcc uaccucaacg ccgucguggc guccgcccug     3060

cugaagaagu accccaagcu cgagcccgag uucguguacg gcgacuaccc gaaguacaac     3120

agcuuccggg agcgcaaguc cgccaccgag aaggucuacu ucuacagcaa caucaugaac     3180

aucuucaaga aguccaucag ccuggccgac gggcggguga ucgagcgccc ccugaucgag     3240

gugaacgagg agaccggcga guccgucugg aacaaggaga gcgaccucgc gacggugcgg     3300

cgcgugcugu ccuaccccca ggucaacgug gugaagaagg ucgaggagca gaaccacggg     3360

cuggaccggg gcaagcccaa gggccucuuc aacgccaacc ugagcuccaa gccgaagccc     3420

aacagcaacg agaaccuggu gggggccaag gaguaccucg accccaagaa guacggcggg     3480

uacgccggca ucuccaacag cuucaccgug cuggucaagg ggaccaucga gaagggcgcc     3540

aagaagaaga ucaccaacgu gcuggaguuc cagggcaucu ccauccucga ccgcaucaac     3600

uaccggaagg acaagcugaa cuuccugcuc gagaaggggu acaaggacau cgagcugauc     3660

aucgagcugc cgaaguacag ccucuucgag cugagcgacg gcucccgccg caugcuggcg     3720

agcauccucu ccacgaacaa caagcgcggg gagauccaca agggcaacca gaucuuccug     3780

agccagaagu ucgugaagcu gcucuaccac gccaagcgga ucuccaacac caucaacgag     3840

aaccaccgga aguacgucga gaaccacaag aaggaguucg aggagcuguu cuacuacauc     3900

cuggaguuca acgagaacua cgugggggcc aagaagaacg gcaagcuccu gaacagcgcc     3960

uuccaguccu ggcagaacca cagcaucgac gagcugugcu ccagcuucau cggccccacc     4020

ggguccgagc gcaagggccu cuucgagcug accagccggg gguccgccgc ggacuucgag     4080

uuccugggcg ugaagauccc ccgcuaccgg gacuacacgc ccagcucccu ccugaaggac     4140

gccacccuga uccaccagag cgucaccggg cucuacgaga cccgcaucga ccuggccaag     4200

cugggcgagg gcaagcggcc ggccgccacg aagaaggcgg ggcaggccaa gaagaagaag     4260

uaauga                                                                4266


<210>  2320
<211>  3348
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2320
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc ggccgccuuc       60

aagcccaaca gcaucaacua cauccugggc cucgacaucg ggaucgccuc cgugggcugg      120

gccauggugg agaucgacga ggaggagaac cccauccggc ugaucgaccu ggggguccgc      180

guguucgagc gggcggaggu gcccaagacc ggcgacagcc ucgccauggc ccgccggcug      240

gcccgcuccg uccggcgccu gacccggcgc cgggcgcacc gccuccugcg gacgcgccgg      300

cugcucaagc gcgagggcgu gcugcaggcc gccaacuucg acgagaacgg gcugaucaag      360

agccucccga acacccccug gcagcugcgg gccgccgcgc uggaccgcaa gcucacgccc      420

cuggaguggu ccgccgugcu gcuccaccug aucaagcacc ggggcuaccu gagccagcgc      480

aagaacgagg gggagaccgc cgacaaggag cucggcgccc ugcugaaggg ggucgccggc      540

aacgcgcacg cccuccagac cggcgacuuc cggacccccg ccgagcuggc ccugaacaag      600

uucgagaagg aguccgggca cauccgcaac cagcgcagcg acuacuccca caccuucagc      660

cggaaggacc uccaggccga gcugauccuc cuguucgaga agcagaagga guucggcaac      720

ccgcacgugu ccgggggccu gaaggagggc aucgagaccc ugcucaugac gcagcgcccc      780

gcgcugagcg gggacgccgu gcagaagaug cugggccacu gcaccuucga gcccgccgag      840

cccaaggccg ccaagaacac cuacaccgcg gagcgguuca ucuggcucac gaagcugaac      900

aaccugcgca uccucgagca gggguccgag cggccgcuga ccgacaccga gcgcgccacc      960

cugauggacg agcccuaccg caagagcaag cucacguacg cccaggcccg gaagcugcug     1020

ggccucgagg acaccgcguu cuucaagggg cugcgguacg gcaaggacaa cgccgaggcc     1080

uccacccuga uggagaugaa ggccuaccac gccaucagcc gcgcgcucga gaaggagggc     1140

cugaaggaca agaagucccc gcugaaccug agccccgagc uccaggacga gaucgggacc     1200

gccuucuccc uguucaagac ggacgaggac aucaccggcc ggcugaagga ccgcauccag     1260

ccggagaucc ucgaggcccu gcugaagcac aucagcuucg acaaguucgu ccagaucagc     1320

cucaaggccc ugcggcgcau cgugccccug auggagcagg ggaagcggua cgacgaggcc     1380

ugcgcggaga ucuacggcga ccacuacggg aagaagaaca ccgaggagaa gaucuaccuc     1440

ccgcccaucc ccgccgacga gauccgcaac cccguggucc ugcgggcccu gucccaggcc     1500

cgcaagguga ucaacggcgu gguccggcgc uacggcagcc ccgcccgcau ccacaucgag     1560

accgcgcggg agguggggaa guccuucaag gaccgcaagg agaucgagaa gcggcaggag     1620

gagaaccgca aggaccggga gaaggccgcc gccaaguucc gcgaguacuu ccccaacuuc     1680

gugggcgagc ccaagagcaa ggacauccuc aagcugcggc uguacgagca gcagcacggg     1740

aagugccucu acuccggcaa ggagaucaac cuggggcgcc ugaacgagaa gggcuacguc     1800

gagaucgacc acgcgcuccc cuucagccgg acgugggacg acuccuucaa caacaaggug     1860

cuggugcugg gcagcgagaa ccagaacaag gggaaccaga cccccuacga guacuucaac     1920

ggcaaggaca acucccgcga guggcaggag uucaaggccc gggucgagac cagccgcuuc     1980

ccccggucca agaagcagcg cauccuccug cagaaguucg acgaggacgg guucaaggag     2040

cggaaccuga acgacacccg cuacgugaac cgguuccucu gccaguucgu cgccgaccgc     2100

augcggcuga cgggcaaggg caagaagcgc guguucgcca gcaacgggca gaucaccaac     2160

cugcuccggg gcuucugggg gcugcgcaag gugcgcgccg agaacgaccg gcaccacgcg     2220

cuggacgccg ucgugguggc cugcuccacc gucgccaugc agcagaagau cacccgcuuc     2280

gugcgguaca aggagaugaa cgccuucgac ggcaagacga ucgacaagga gaccggggag     2340

gugcuccacc agaagaccca cuucccgcag cccugggagu ucuucgcgca ggaggucaug     2400

auccgcgugu ucggcaagcc cgacggcaag ccggaguucg aggaggccga cacccuggag     2460

aagcugcgga cgcuccuggc cgagaagcug agcucccgcc ccgaggccgu gcacgaguac     2520

gucacgcccc ucuucgugag ccgggccccc aaccgcaaga uguccgggca gggccacaug     2580

gagaccguca agagcgcgaa gcggcuggac gagggggugu ccgugcugcg cgugccgcuc     2640

acccagcuga agcugaagga ccucgagaag auggucaacc gggagcgcga gcccaagcug     2700

uacgaggccc ugaaggcccg gcucgaggcc cacaaggacg accccgcgaa ggccuucgcc     2760

gagcccuucu acaaguacga caaggccggc aaccgcaccc agcaggucaa ggccgugcgg     2820

guggagcagg uccagaagac cggggugugg gugcgcaacc acaacggcau cgcggacaac     2880

gccaccaugg ugcgggugga cgucuucgag aagggcgaca aguacuaccu ggugccgauc     2940

uacagcuggc aggucgccaa ggggauccug cccgaccgcg ccguggugca gggcaaggac     3000

gaggaggacu ggcagcucau cgacgacagc uucaacuuca aguucucccu gcaccccaac     3060

gaccuggugg aggucaucac caagaaggcc cggauguucg gguacuucgc gagcugccac     3120

cgcggcaccg ggaacaucaa cauccgcauc cacgaccucg accacaagau cggcaagaac     3180

ggcauccugg aggggaucgg cgugaagacg gcccuguccu uccagaagua ccagaucgac     3240

gagcucggga aggagauccg gcccugccgc cugaagaagc ggccgcccgu gcgcaagcgg     3300

cccgccgcca ccaagaaggc cggccaggcg aagaagaaga aguaauga                  3348


<210>  2321
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2321
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gaagaagccc       60

uacaccaucg gccuggacau cgggaccaac agcgugggcu gggccgugcu cacggaccag      120

uacgaccugg ucaagcggaa gaugaagauc gccggggacu ccgagaagaa gcagaucaag      180

aagaacuucu ggggcgugcg ccuguucgac gagggccaga ccgccgccga ccggcgcaug      240

gcgcggaccg cccgccggcg caucgagcgg cgccggaacc gcaucagcua ccuccagggg      300

aucuucgccg aggagauguc caagaccgac gccaacuucu ucugccggcu gagcgacucc      360

uucuacgugg acaacgagaa gcgcaacagc cggcaccccu ucuucgcgac gaucgaggag      420

gaggucgagu accacaagaa cuaccccacc aucuaccacc uccgcgagga gcuggugaac      480

uccagcgaga aggccgaccu gcggcuggug uaccucgccc uggcccacau caucaaguac      540

cgcggcaacu uccugaucga gggggcccuc gacacccaga acaccuccgu cgacggcauc      600

uacaagcagu ucauccagac guacaaccag guguucgcga gcgggaucga ggacggcucc      660

cugaagaagc uggaggacaa caaggacgug gccaagaucc ucgucgagaa ggugacccgg      720

aaggagaagc uggagcgcau ccugaagcuc uacccgggcg agaaguccgc cgggauguuc      780

gcccaguuca ucagccugau cgugggcagc aaggggaacu uccagaagcc cuucgaccug      840

aucgagaagu ccgacaucga gugcgccaag gacagcuacg aggaggaccu cgagucccug      900

cuggcgcuca ucggcgacga guacgccgag cuguucgucg ccgccaagaa cgccuacagc      960

gcgguggugc uguccagcau caucaccguc gccgagaccg agacgaacgc caagcucagc     1020

gccuccauga ucgagcgguu cgacacccac gaggaggacc ugggcgagcu gaaggccuuc     1080

aucaagcucc accuccccaa gcacuacgag gagaucuuca gcaacaccga gaagcacggg     1140

uacgcgggcu acaucgacgg gaagaccaag caggccgacu ucuacaagua caugaagaug     1200

acgcuggaga acaucgaggg cgccgacuac uucaucgcca agaucgagaa ggagaacuuc     1260

cugcgcaagc agcgcaccuu cgacaacggg gcgauccccc accagcugca ccuggaggag     1320

cucgaggcca uccugcacca gcaggccaag uacuacccgu uccugaagga gaacuacgac     1380

aagaucaagu cccucgugac cuuccggauc cccuacuucg ugggcccccu ggccaacggc     1440

cagagcgagu ucgccuggcu gacccgcaag gcggacgggg agauccggcc cuggaacauc     1500

gaggagaagg ucgacuucgg caaguccgcc guggacuuca ucgagaagau gacgaacaag     1560

gacaccuacc ucccgaagga gaacgugcug cccaagcaca gccugugcua ccagaaguac     1620

cucgucuaca acgagcugac caaggugcgc uacaucaacg accaggggaa gaccuccuac     1680

uucagcggcc aggagaagga gcagaucuuc aacgaccugu ucaagcagaa gcggaagguc     1740

aagaagaagg accucgagcu guuccugcgc aacauguccc acguggagag ccccacgauc     1800

gaggggcugg aggacuccuu caacagcucc uacagcaccu accacgaccu ccugaaggug     1860

ggcaucaagc aggagauccu ggacaacccc gucaacaccg agaugcucga gaacaucgug     1920

aagauccuga ccguguucga ggacaagcgg augaucaagg agcagcugca gcaguucucc     1980

gacguccucg acggcguggu gcugaagaag cuggagcgcc ggcacuacac gggguggggc     2040

cgccucagcg ccaagcugcu gauggggauc cgggacaagc agucccaccu caccauccug     2100

gacuaccuga ugaacgacga cggccucaac cgcaaccuga ugcagcugau caacgacagc     2160

aaccucuccu ucaagagcau caucgagaag gagcagguca ccaccgccga caaggacauc     2220

cagagcaucg uggccgaccu ggcggggucc ccggccauca agaagggcau ccugcagagc     2280

cucaagaucg uggacgagcu ggucuccgug augggcuacc cgccccagac gaucgugguc     2340

gagauggccc gggagaacca gaccaccggg aagggcaaga acaacagccg cccccgguac     2400

aagucccugg agaaggccau caaggaguuc gggagccaga uccucaagga gcaccccacc     2460

gacaaccagg agcugcgcaa caaccggcug uaccucuacu accugcagaa cggcaaggac     2520

auguacacgg gccaggaccu ggacauccac aaccucucca acuacgacau cgaccacauc     2580

gugccccaga gcuucaucac cgacaacucc aucgacaacc uggugcugac cagcuccgcc     2640

gggaaccgcg agaagggcga cgacgucccg ccccucgaga ucgugcggaa gcgcaaggug     2700

uucugggaga agcuguacca ggggaaccug augagcaagc gcaaguucga cuaccucacc     2760

aaggcggagc ggggcgggcu gaccgaggcc gacaaggccc gcuucaucca ccggcagcug     2820

gucgagacgc gccagaucac caagaacgug gccaacaucc uccaccagcg guucaacuac     2880

gagaaggacg accacggcaa caccaugaag cagguccgca ucgugacccu gaaguccgcg     2940

cuggugagcc aguuccggaa gcaguuccag cucuacaagg ugcgcgacgu caacgacuac     3000

caccacgccc acgacgccua ccugaacggc gugguggcca acacgcugcu caaggucuac     3060

ccccagcugg agcccgaguu cguguacggg gacuaccacc aguucgacug guucaaggcc     3120

aacaaggcga ccgccaagaa gcaguucuac accaacauca ugcuguucuu cgcccagaag     3180

gaccggauca ucgacgagaa cggcgagauc cucugggaca agaaguaccu ggacaccgug     3240

aagaagguca uguccuaccg ccagaugaac aucgugaaga agacggagau ccagaagggg     3300

gaguucagca aggccaccau caagcccaag ggcaacucca gcaagcugau cccccggaag     3360

accaacuggg acccgaugaa guacgggggc cucgacagcc ccaacauggc cuacgcggug     3420

gucaucgagu acgccaaggg caagaacaag cugguguucg agaagaagau cauccgcgug     3480

accaucaugg agcggaaggc cuucgagaag gacgagaagg ccuuccugga ggagcagggg     3540

uaccgccagc ccaagguccu cgccaagcug ccgaaguaca cgcuguacga gugcgaggag     3600

ggccggcgcc ggaugcucgc guccgccaac gaggcccaga aggggaacca gcagguccug     3660

cccaaccacc uggugacccu ccugcaccac gccgcgaacu gcgaggugag cgacggcaag     3720

ucccuggacu acaucgagag caaccgcgag auguucgccg agcuccuggc ccacgugucc     3780

gaguucgcca agcgguacac ccuggccgag gcgaaccuca acaagaucaa ccagcuguuc     3840

gagcagaaca aggaggggga caucaaggcc aucgcccaga gcuucgugga ccugauggcc     3900

uucaacgcca ugggcgcgcc cgccuccuuc aaguucuucg agaccacgau cgagcgcaag     3960

cgguacaaca accucaagga gcugcugaac agcaccauca ucuaccaguc caucaccggc     4020

cucuacgaga gccgcaagcg ccuggacgac aagcggcccg ccgccaccaa gaaggccggg     4080

caggcgaaga agaagaagua auga                                            4104


<210>  2322
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2322
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gaagaagccc       60

uacagcaucg gccuggacau cgggaccaac uccgugggcu gggccguggu caccgacgac      120

uacaaggugc ccgccaagaa gaugaaggug cucgggaaca cggacaagag ccacaucgag      180

aagaaccugc ugggcgcccu ccuguucgac uccggcaaca ccgccgagga ccggcgccug      240

aagcggaccg cgcgccggcg cuacacccgg cgccggaacc gcauccucua ccugcaggag      300

aucuucagcg aggagauggg gaaggucgac gacuccuucu uccaccggcu ggaggacagc      360

uuccucguga cggaggacaa gcgcggcgag cggcacccca ucuucgggaa ccuggaggag      420

gaggugaagu accacgagaa cuucccgacc aucuaccacc uccgccagua ccuggccgac      480

aaccccgaga aggucgaccu gcggcuggug uaccucgccc uggcccacau caucaaguuc      540

cgcggccacu uccugaucga ggggaaguuc gacacccgga acaacgacgu gcagcgccuc      600

uuccaggagu uccuggcggu cuacgacaac accuucgaga acuccagccu gcaggagcag      660

aacguccagg uggaggagau ccucacggac aagaucucca agagcgccaa gaaggaccgg      720

gugcugaagc uguuccccaa cgagaagucc aacggccgcu ucgccgaguu ccucaagcug      780

aucgugggca accaggccga cuucaagaag cacuucgagc uggaggagaa ggccccgcuc      840

caguucagca aggacaccua cgaggaggag cuggaggugc ugcucgcgca gaucggggac      900

aacuacgccg agcuguuccu guccgccaag aagcucuacg acagcauccu gcuguccggc      960

auccucaccg ucaccgacgu ggggacgaag gccccgcuga gcgccuccau gauccagcgg     1020

uacaacgagc accagaugga ccuggcgcag cucaagcagu ucauccgcca gaagcugagc     1080

gacaaguaca acgagguguu cuccgacguc agcaaggacg gcuacgccgg cuacaucgac     1140

gggaagacca accaggaggc cuucuacaag uaccugaagg gccuccugaa caagaucgag     1200

gggagcggcu acuuccugga caagaucgag cgcgaggacu uccuccggaa gcagcgcacc     1260

uucgacaacg gguccauccc ccaccagauc caccuccagg agaugcgggc caucauccgc     1320

cggcaggccg aguucuaccc cuuccuggcg gacaaccagg accgcaucga gaagcugcug     1380

accuuccgga uccccuacua cgugggcccg cuggcccgcg gcaagagcga cuucgccugg     1440

cugucccgca agagcgccga caagaucacg cccuggaacu ucgacgagau cguggacaag     1500

gaguccagcg ccgaggcguu caucaaccgg augaccaacu acgaccucua ccugcccaac     1560

cagaaggucc ugcccaagca cucccuccug uacgagaagu ucaccgugua caacgagcug     1620

accaagguca aguacaagac ggagcagggg aagaccgccu ucuucgacgc caacaugaag     1680

caggagaucu ucgacggcgu guucaaggug uaccggaagg ucaccaagga caagcucaug     1740

gacuuccugg agaaggaguu cgacgaguuc cgcaucgugg accugaccgg gcucgacaag     1800

gagaacaagg uguucaacgc cagcuacggc acguaccacg accugugcaa gauccuggac     1860

aaggacuucc ucgacaacuc caagaacgag aagauccugg aggacaucgu ccugacccuc     1920

acccuguucg aggaccggga gaugauccgc aagcggcugg agaacuacag cgaccuccug     1980

accaaggagc aggucaagaa gcuggagcgc cggcacuaca cggggugggg ccgccucucc     2040

gcggagcuga uccacggcau ccggaacaag gagagccgca agaccauccu ggacuaccuc     2100

aucgacgacg ggaacuccaa ccgcaacuuc augcagcuga ucaacgacga cgcccugagc     2160

uucaaggagg agaucgccaa ggcccaggug aucggcgaga ccgacaaccu caaccaggug     2220

guguccgaca ucgccgggag cccggcgauc aagaagggca uccugcaguc ccugaagauc     2280

guggacgagc ucgucaagau cauggggcac cagcccgaga acaucguggu ggagauggcc     2340

cgggagaacc aguucaccaa ccagggccgc cggaacagcc agcagcgccu gaagggccug     2400

acggacucca ucaaggaguu cgggagccag auccucaagg agcaccccgu cgagaacagc     2460

cagcugcaga acgaccggcu guuccucuac uaccugcaga acggccgcga cauguacacc     2520

ggggaggagc uggacaucga cuaccucucc caguacgaca ucgaccacau caucccccag     2580

gccuucauca aggacaacag caucgacaac cgggugcuga ccuccagcaa ggagaaccgc     2640

ggcaaguccg acgacgugcc gagcaaggac gucgugcgga agaugaaguc cuacuggagc     2700

aagcugcucu ccgccaagcu gaucacccag cgcaaguucg acaaccugac gaaggccgag     2760

cggggcgggc ucaccgacga cgacaaggcg ggcuucauca agcgccagcu gguggagacc     2820

cggcagauca ccaagcacgu cgcccgcauc cuggacgagc gguucaacac cgagacggac     2880

gagaacaaca agaagauccg ccaggugaag aucgugaccc ucaagagcaa ccuggucucc     2940

aacuuccgga aggaguucga gcuguacaag gugcgcgaga ucaacgacua ccaccacgcc     3000

cacgacgccu accucaacgc cgugaucggg aaggcgcugc ugggcgucua cccccagcuc     3060

gagcccgagu ucguguacgg ggacuacccc cacuuccacg gccacaagga gaacaaggcc     3120

accgccaaga aguucuucua cagcaacauc augaacuucu ucaagaagga cgacgugcgg     3180

accgacaaga acggcgagau caucuggaag aaggacgagc acaucuccaa caucaagaag     3240

guccugagcu acccccaggu gaacaucgug aagaaggucg aggagcagac cgggggcuuc     3300

uccaaggaga gcauccugcc caaggggaac uccgacaagc ucaucccccg caagacgaag     3360

aaguucuacu gggacaccaa gaaguacggc ggguucgaca gccccaucgu ggccuacagc     3420

auccugguga ucgcggacau cgagaagggc aaguccaaga agcugaagac cgucaaggcc     3480

cucgugggcg ugacgaucau ggagaagaug accuucgagc gggacccggu cgccuuccug     3540

gagcgcaagg gguaccgcaa cgugcaggag gagaacauca ucaagcugcc caaguacagc     3600

cucuucaagc uggagaacgg ccggaagcgc cugcucgccu ccgcccggga gcugcagaag     3660

gggaacgaga ucgugcugcc caaccaccuc ggcacccugc uguaccacgc gaagaacauc     3720

cacaaggucg acgagccgaa gcaccucgac uacguggaca agcacaagga cgaguucaag     3780

gagcugcugg acguggucag caacuucucc aagaaguaca cccucgccga ggggaaccug     3840

gagaagauca aggagcugua cgcccagaac aacggcgagg accucaagga gcuggccagc     3900

uccuucauca accugcucac guucaccgcc aucggcgcgc ccgccaccuu caaguucuuc     3960

gacaagaaca ucgaccgcaa gcgguacacc agcacgaccg agauccugaa cgccacccug     4020

auccaccagu ccaucaccgg gcucuacgag acgcgcaucg accugaacaa gcugggcggg     4080

gacaagcggc ccgccgccac caagaaggcg ggccaggcca agaagaagaa guaauga        4137


<210>  2323
<211>  3270
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2323
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gcagaccacc       60

aaccugagcu acauccucgg ccuggaccug gggaucgccu ccgugggcug ggccgugguc      120

gagaucaacg agaacgagga ccccaucggg cucaucgacg ugggcgugcg gaucuucgag      180

cgcgccgagg uccccaagac gggcgagagc cuggcccugu cccggcgccu cgcgcggagc      240

acccgccggc ugauccgccg gcgcgcccac cggcugcucc uggccaagcg cuuccugaag      300

cgggagggga uccucuccac caucgaccug gagaagggcc ugcccaacca ggccugggag      360

cuccgcgugg cggggcugga gcggcgccug agcgccaucg aguggggcgc cgugcuccug      420

caccugauca agcaccgggg guaccucucc aagcgcaaga acgagagcca gaccaacaac      480

aaggagcugg gcgcccugcu cuccggcguc gcccagaacc accagcugcu gcagagcgac      540

gacuaccgga cgccggcgga gcucgcccug aagaaguucg ccaaggagga ggggcacauc      600

cgcaaccagc ggggcgccua cacccacacc uucaaccgcc uggaccuccu ggccgagcug      660

aaccuccugu ucgcgcagca gcaccaguuc gggaaccccc acugcaagga gcacauccag      720

caguacauga ccgagcugcu cauguggcag aagcccgccc uguccggcga ggccauccug      780

aagaugcucg gcaagugcac gcacgagaag aacgaguuca aggccgccaa gcacaccuac      840

agcgcggagc gcuucgugug gcugaccaag cugaacaacc uccggauccu ggaggacggg      900

gccgagcgcg cccugaacga ggaggagcgg cagcuccuga ucaaccaccc cuacgagaag      960

uccaagcuga ccuacgccca ggugcgcaag cuccugggcc ugagcgagca ggccaucuuc     1020

aagcaccucc gguacuccaa ggagaacgcg gagagcgcca cguucaugga gcucaaggcc     1080

uggcacgcca uccgcaaggc gcuggagaac caggggcuga aggacaccug gcaggaccuc     1140

gccaagaagc cggaccugcu ggacgagauc ggcaccgccu ucucccucua caagaccgac     1200

gaggacaucc agcaguaccu gacgaacaag guccccaaca gcgugaucaa cgcccugcuc     1260

gugucccuga acuucgacaa guucaucgag cugagccuca agagccugcg gaagauccug     1320

ccccucaugg agcaggggaa gcgcuacgac caggccugcc gggagaucua cggccaccac     1380

uacggcgagg cgaaccagaa gaccucccag cugcugcccg ccaucccggc ccaggagauc     1440

cgcaaccccg ucgugcuccg gacccugagc caggcccgca aggugaucaa cgccaucauc     1500

cggcaguacg ggucccccgc gcgcguccac aucgagaccg gccgggagcu gggcaagagc     1560

uucaaggagc gccgggagau ccagaagcag caggaggaca accgcacgaa gcgggagucc     1620

gccgugcaga aguucaagga gcuguucagc gacuucucca gcgagcccaa guccaaggac     1680

auccugaagu uccgccugua cgagcagcag cacgggaagu gccucuacag cgggaaggag     1740

aucaacaucc accgccugaa cgagaagggc uacguggaga ucgaccacgc ccugccguuc     1800

ucccggaccu gggacgacag cuucaacaac aagguccucg ugcuggccuc cgagaaccag     1860

aacaagggca accagacccc cuacgagugg cugcagggga agaucaacag cgagcgcugg     1920

aagaacuucg uggcccucgu ccugggcucc cagugcagcg cggccaagaa gcagcggcug     1980

cucacccagg ugaucgacga caacaaguuc aucgaccgca accugaacga cacgcgguac     2040

aucgcccgcu uccuguccaa cuacauccag gagaaccucc ugcuggucgg gaagaacaag     2100

aagaacgugu ucacccccaa cggccagauc accgcccucc ugcggagccg cugggggcug     2160

aucaaggcgc gggagaacaa caaccgccac cacgcccucg acgccaucgu ggucgccugc     2220

gccacccccu ccaugcagca gaagaucacg cgguucaucc gcuucaagga ggugcacccg     2280

uacaagaucg agaaccggua cgagauggug gaccaggaga gcggcgagau caucagcccc     2340

cacuuccccg agcccugggc guacuuccgc caggagguca acauccgggu guucgacaac     2400

cacccggaca ccgugcugaa ggagaugcug cccgaccgcc cccaggccaa ccaccaguuc     2460

guccagcccc ucuucguguc ccgggccccg acccgcaaga ugagcggcca ggggcacaug     2520

gagaccauca aguccgccaa gcggcuggcc gagggcauca gcgugcugcg caucccccuc     2580

acgcagcuga agcccaaccu gcucgagaac auggucaaca aggagcggga gccggcgcug     2640

uacgccgggc ugaaggcccg ccucgccgag uucaaccagg accccgccaa ggcguucgcc     2700

acccccuucu acaagcaggg cggccagcag gucaaggcca uccgcgugga gcagguccag     2760

aaguccgggg ugcuggugcg ggagaacaac ggcguggccg acaacgcgag caucgugcgc     2820

accgacgugu ucaucaagaa caacaaguuc uuccuggucc ccaucuacac cuggcagguc     2880

gccaagggga uccucccgaa caaggccauc guggcccaca agaacgagga cgagugggag     2940

gagauggacg agggcgccaa guucaaguuc ucccuguucc ccaacgaccu gguggagcuc     3000

aagacgaaga aggaguacuu cuucggguac uacaucggcc uggaccgggc gaccggcaac     3060

aucagccuga aggagcacga cggggagauc uccaagggca aggacggggu guaccgcgug     3120

ggcgucaagc ucgcccugag cuucgagaag uaccaggugg acgagcuggg gaagaaccgg     3180

cagaucugcc gcccccagca gcggcagccc gugcgcaagc ggccggccgc caccaagaag     3240

gccggccagg cgaagaagaa gaaguaauga                                      3270


<210>  2324
<211>  3348
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2324
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc ggccgccuuc       60

aagcccaacc ccaucaacua cauccugggc cucgacaucg ggaucgccag cgugggcugg      120

gccauggugg agaucgacga ggacgagaac cccaucugcc ugaucgaccu ggggguccgg      180

guguucgagc gcgcggaggu gccgaagacc ggcgacuccc ucgccauggc ccggcgccug      240

gcccggagcg uccgccggcu gacccgccgg cgcgcgcacc ggcuccugcg cgcccggcgc      300

cugcucaagc gggagggcgu gcugcaggcc gccgacuucg acgagaacgg gcugaucaag      360

ucccucccca acacgcccug gcagcugcgc gccgcggccc uggaccggaa gcucacgccc      420

cuggagugga gcgccgugcu gcuccaccug aucaagcacc gcggcuaccu gucccagcgg      480

aagaacgagg gggagaccgc cgacaaggag cucggcgccc ugcugaaggg ggucgcggac      540

aacgcccacg cccuccagac cggcgacuuc cgcaccccgg ccgagcuggc ccugaacaag      600

uucgagaagg agagcggcca cauccggaac cagcgcgggg acuacuccca caccuucagc      660

cgcaaggacc uccaggcgga gcugauccuc cucuucgaga agcagaagga guucggcaac      720

ccccacgugu ccgggggccu gaaggagggc aucgagaccc ugcucaugac ccagcggccc      780

gcccugagcg gggacgccgu gcagaagaug cugggccacu gcacguucga gcccgccgag      840

ccgaaggccg cgaagaacac cuacaccgcc gagcgcuuca ucuggcucac caagcugaac      900

aaccugcgga uccucgagca gggguccgag cgcccccuga cggacaccga gcgggccacc      960

cugauggacg agcccuaccg caagagcaag cucaccuacg cccaggcgcg gaagcugcug     1020

ggccucgagg acacggccuu cuucaagggg cugcgcuacg gcaaggacaa cgccgaggcc     1080

uccacccuga uggagaugaa ggccuaccac gcgaucagcc gggcccucga gaaggagggc     1140

cugaaggaca agaagucccc gcugaaccug agccccgagc uccaggacga gaucgggacc     1200

gccuucuccc uguucaagac cgacgaggac aucacgggcc gccugaagga ccggauccag     1260

cccgagaucc ucgaggcccu gcugaagcac aucagcuucg acaaguucgu ccagaucucc     1320

cucaaggccc ugcgccggau cgugccccug auggagcagg ggaagcgcua cgacgaggcg     1380

ugcgccgaga ucuacggcga ccacuacggg aagaagaaca ccgaggagaa gaucuaccug     1440

cccccgaucc ccgccgacga gauccggaac cccguggucc ugcgcgcccu gagccaggcc     1500

cggaagguga ucaacggcgu gguccgccgg uacggcagcc ccgcgcgcau ccacaucgag     1560

accgcccgcg agguggggaa guccuucaag gaccggaagg agaucgagaa gcgccaggag     1620

gagaaccgga aggaccgcga gaaggccgcc gcgaaguucc gggaguacuu cccgaacuuc     1680

gugggcgagc ccaagagcaa ggacauccuc aagcugcgcc uguacgagca gcagcacggg     1740

aagugccucu acuccggcaa ggagaucaac cuggggcggc ugaacgagaa gggcuacguc     1800

gagaucgacc acgcccuccc cuucagccgc accugggacg acuccuucaa caacaaggug     1860

cuggugcugg gcagcgagaa ccagaacaag gggaaccaga cgcccuacga guacuucaac     1920

ggcaaggaca acucccggga guggcaggag uucaaggccc gcgucgagac cagccgguuc     1980

ccgcgcucca agaagcagcg gauccuccug cagaaguucg acgaggacgg guucaaggag     2040

cgcaaccuga acgacacccg guacgugaac cgcuuccucu gccaguucgu cgccgaccgg     2100

augcgccuga ccggcaaggg caagaagcgg guguucgcca gcaacgggca gaucacgaac     2160

cugcuccgcg gcuucugggg gcugcggaag gugcgcgcgg agaacgaccg ccaccacgcc     2220

cuggacgccg ucgugguggc cugcuccacc gucgccaugc agcagaagau cacccgguuc     2280

gugcgcuaca aggagaugaa cgcguucgac ggcaagacca ucgacaagga gacgggggag     2340

gugcuccacc agaagaccca cuucccccag cccugggagu ucuucgccca ggaggucaug     2400

auccgggugu ucggcaagcc ggacggcaag cccgaguucg aggaggccga cacccccgag     2460

aagcugcgca cccugcucgc cgagaagcug agcucccggc ccgaggccgu gcacgaguac     2520

gucacgccgc uguucgugag ccgcgcgccc aaccggaaga uguccgggca gggccacaug     2580

gagaccguca agagcgccaa gcgccucgac gagggggugu ccgugcugcg ggugccccug     2640

acccagcuca agcugaagga ccuggagaag auggucaacc gcgagcggga gcccaagcuc     2700

uacgaggccc ugaaggcccg ccuggaggcg cacaaggacg acccggccaa ggccuucgcc     2760

gagcccuucu acaaguacga caaggccggc aaccggaccc agcaggucaa ggcggugcgc     2820

guggagcagg uccagaagac cggggugugg gugcggaacc acaacggcau cgccgacaac     2880

gccacgaugg ugcgcgugga cgucuucgag aagggcgaca aguacuaccu cgugcccauc     2940

uacagcuggc aggucgccaa ggggauccug cccgaccggg ccguggugca gggcaaggac     3000

gaggaggacu ggcagcugau cgacgacucc uucaacuuca aguucagccu ccacccgaac     3060

gaccuggugg aggucaucac caagaaggcg cgcauguucg gguacuucgc cagcugccac     3120

cggggcaccg ggaacaucaa cauccgcauc cacgaccugg accacaagau cggcaagaac     3180

ggcauccucg aggggaucgg cgugaagacc gcccuguccu uccagaagua ccagaucgac     3240

gagcugggga aggagauccg ccccugccgg cucaagaagc gcccgcccgu gcggaagcgc     3300

cccgccgcca cgaagaaggc gggccaggcc aagaagaaga aguaauga                  3348


<210>  2325
<211>  3354
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2325
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gaaguaccac       60

gugggcaucg acguggggac cuucagcguc ggccuggccg ccaucgaggu ggacgacgcc      120

gggaugccca ucaagacccu gucccuggug agccacaucc acgacuccgg ccuggacccc      180

gacgagauca agagcgccgu cacgcggcuc gcguccagcg gcaucgcccg ccggacccgc      240

cggcuguacc gccggaagcg ccggcgccug cagcagcucg acaaguucau ccagcggcag      300

ggguggcccg ugaucgagcu ggaggacuac uccgacccgc uguaccccug gaaggugcgc      360

gccgagcucg ccgcgagcua caucgccgac gagaaggagc ggggcgagaa gcuguccguc      420

gcccugcgcc acaucgcccg gcaccgcggg uggcggaacc ccuacgccaa ggugagcucc      480

cucuaccugc ccgacggccc gagcgacgcg uucaaggcca uccgcgagga gaucaagcgg      540

gccuccgggc agcccgugcc cgagaccgcc accgucggcc agauggugac gcugugcgag      600

cucggcaccc ugaagcugcg cggggagggc ggggugcuca gcgcccggcu gcagcagucc      660

gacuacgcgc gcgagaucca ggagaucugc cgcaugcagg agaucggcca ggagcuguac      720

cggaagauca ucgacgucgu guucgccgcc gagagcccca agggcuccgc cagcucccgc      780

guggggaagg acccgcucca gcccggcaag aaccgggccc ugaaggcgag cgacgccuuc      840

cagcgcuacc ggaucgccgc ccugaucggg aaccuccgcg uccgggugga cggcgagaag      900

cgcauccuga gcguggagga gaagaaccug gucuucgacc accucgugaa ccugaccccc      960

aagaaggagc ccgagugggu gaccaucgcc gagauccugg ggaucgaccg gggccagcuc     1020

aucggcacgg cgaccaugac cgacgacggg gagcgcgccg gcgcccggcc gcccacccac     1080

gacacgaacc gcuccaucgu caacagccgg aucgcgcccc ugguggacug guggaagacc     1140

gccuccgccc uggagcagca cgccaugguc aaggcccuca gcaacgccga gguggacgac     1200

uucgacuccc ccgagggggc gaaggugcag gccuucuucg ccgaccugga cgacgacguc     1260

cacgccaagc uggacagccu ccaccucccg gugggccgcg ccgcguacuc cgaggacacc     1320

cuggugcggc ucacccgccg gaugcugagc gacggggucg accuguacac ggcccgccuc     1380

caggaguucg gcaucgagcc cuccuggacc ccgcccacgc cccggaucgg cgagcccgug     1440

gggaaccccg ccguggaccg cguccugaag accgugagcc gcuggcugga guccgccacc     1500

aagaccuggg gcgcccccga gcgggugauc aucgagcacg uccgcgaggg guucgugacc     1560

gagaagcggg cgcgcgagau ggacggcgac augcggcgcc gggccgcccg caacgccaag     1620

cuguuccagg agaugcagga gaagcugaac gugcagggga agccgagccg ggcggaccug     1680

uggcgcuacc aguccgucca gcggcagaac ugccagugcg ccuacugcgg cagccccauc     1740

accuucucca acagcgagau ggaccacauc gugccccgcg ccggccaggg guccacgaac     1800

acccgggaga accucguggc cgucugccac cgcugcaacc agagcaaggg caacaccccg     1860

uucgccaucu gggcgaagaa caccuccauc gaggggguga gcgugaagga ggccgucgag     1920

cggacgcgcc acugggugac cgacaccggc augcggagca ccgacuucaa gaaguucacg     1980

aaggccgugg ucgagcgcuu ccagcgggcc accauggacg aggagaucga cgcccgcucc     2040

auggagagcg uggcguggau ggccaacgag cugcgguccc gcguggccca gcacuucgcc     2100

agccacggca ccaccguccg cguguaccgg gggucccuga cggccgaggc gcgccgggcc     2160

agcggcaucu ccgggaagcu gaaguucuuc gacggcgugg gcaagagccg ccucgaccgg     2220

cgccaccacg ccaucgacgc cgcggucauc gccuucaccu ccgacuacgu ggccgagacc     2280

cuggccgugc ggagcaaccu gaagcagucc caggcccacc gccaggaggc gccccagugg     2340

cgggaguuca ccgggaagga cgccgagcac cgcgccgccu ggcgggucug gugccagaag     2400

auggagaagc ucagcgcccu gcugaccgag gaccuccgcg acgaccgggu gguggucaug     2460

uccaacgugc gccugcggcu gggcaacggg agcgcgcaca aggagacgau cggcaagcuc     2520

uccaagguga agcugagcuc ccagcugagc gucagcgaca ucgacaaggc cuccagcgag     2580

gcccucuggu gcgcccugac ccgcgagccc ggguucgacc ccaaggaggg ccugccggcc     2640

aaccccgagc ggcacauccg cgugaacggg acccacgugu acgcgggcga caacaucggc     2700

cucuuccccg ucuccgccgg gagcaucgcc cugcggggcg gguacgccga gcugggcucc     2760

agcuuccacc acgcccgcgu guacaagauc accuccggga agaagcccgc guucgccaug     2820

cuccgcgugu acacgaucga ccugcugccg uaccggaacc aggaccucuu cagcgucgag     2880

cugaagcccc agaccauguc caugcgccag gccgagaaga agcugcggga cgcccucgcg     2940

accggcaacg ccgaguaccu gggcuggcug gugguggacg acgagcucgu cguggacacc     3000

agcaagaucg ccacggacca ggugaaggcc gucgaggccg agcuggggac cauccgccgg     3060

uggcgcgugg acggcuucuu cucccccagc aagcugcggc uccgcccccu gcagaugucc     3120

aaggagggga ucaagaagga gagcgcgccg gagcugucca agaucaucga ccggcccggc     3180

uggcuccccg ccgucaacaa gcuguucagc gacgggaacg ugaccguggu ccgccgggac     3240

ucccugggcc gcgugcggcu cgagagcacc gcccaccucc ccgugacgug gaagguccag     3300

aagcgcccgg ccgccaccaa gaaggcgggc caggccaaga agaagaagua auga           3354


<210>  2326
<211>  3054
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2326
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc ggcccggauc       60

cuggccuucg acaucggcau cagcuccauc gggugggccu ucagcgagaa cgacgagcuc      120

aaggacugcg gcgugcgcau cuucaccaag guggagaacc ccaagaccgg ggagucccug      180

gcccugcccc ggcgccucgc gcggagcgcc cgcaagcggc uggcccgccg gaaggcccgc      240

cugaaccacc ucaagcaccu gaucgcgaac gaguucaagc ugaacuacga ggacuaccag      300

uccuucgacg agagccucgc caaggccuac aagggcuccc ugaucagccc cuacgagcug      360

cgguuccgcg cccucaacga gcugcugucc aagcaggacu ucgcccgggu cauccuccac      420

aucgcgaagc gccggggcua cgacgacauc aagaacagcg acgacaagga gaagggggcc      480

auccugaagg ccaucaagca gaacgaggag aagcuggcca acuaccaguc cgugggcgag      540

uaccucuaca aggaguacuu ccagaaguuc aaggagaaca gcaaggaguu cacgaacgug      600

cgcaacaaga aggaguccua cgagcggugc aucgcccaga gcuuccugaa ggacgagcug      660

aagcucaucu ucaagaagca gcgcgaguuc ggguucuccu ucagcaagaa guucgaggag      720

gagguccugu ccguggcguu cuacaagcgg gcccugaagg acuucagcca ccucgugggc      780

aacugcuccu ucuucaccga cgagaagcgc gccccgaaga acagcccccu ggccuucaug      840

uucgucgccc ugacccggau caucaaccuc cugaacaacc ugaagaacac cgaggggauc      900

cucuacacga aggacgaccu gaacgcgcug cucaacgagg ugcugaagaa cggcacccug      960

accuacaagc agaccaagaa gcuccugggc cugagcgacg acuacgaguu caagggggag     1020

aagggcacgu acuucaucga guucaagaag uacaaggagu ucaucaaggc ccucggggag     1080

cacaaccugu cccaggacga ccugaacgag aucgccaagg acaucacccu caucaaggac     1140

gagaucaagc ugaagaaggc ccuggccaag uacgaccuca accagaacca gaucgacagc     1200

cuguccaagc uggaguucaa ggaccaccuc aacaucagcu ucaaggcgcu gaagcuggug     1260

acgccccuga ugcucgaggg caagaaguac gacgaggccu gcaacgagcu gaaccugaag     1320

gucgccauca acgaggacaa gaaggacuuc cuccccgccu ucaacgagac cuacuacaag     1380

gacgagguga ccaacccggu gguccugcgc gcgaucaagg aguaccgcaa ggugcugaac     1440

gcccuccuga agaaguacgg caaggugcac aagaucaaca ucgagcuggc ccgggagguc     1500

gggaagaacc acucccagcg cgccaagauc gagaaggagc agaacgagaa cuacaaggcc     1560

aagaaggacg cggagcucga gugcgagaag cugggccuga agaucaacag caagaacauc     1620

cucaagcugc ggcuguucaa ggagcagaag gaguucugcg ccuacuccgg ggagaagauc     1680

aagaucagcg accuccagga cgagaagaug cuggagaucg accacaucua ccccuacucc     1740

cgcagcuucg acgacuccua caugaacaag gugcuggugu ucaccaagca gaaccaggag     1800

aagcucaacc agacccccuu cgaggccuuc ggcaacgaca gcgccaagug gcagaagauc     1860

gagguccugg ccaagaaccu gcccaccaag aagcagaagc ggauccucga caagaacuac     1920

aaggacaagg agcagaagaa cuucaaggac cgcaaccuga acgacacgcg guacaucgcg     1980

cgccuggugc ucaacuacac caaggacuac cuggacuucc ugccgcucuc cgacgacgag     2040

aacaccaagc ugaacgacac ccagaagggg agcaaggugc acgucgaggc caaguccggc     2100

augcugacga gcgcccuccg gcacaccugg ggcuucuccg ccaaggaccg caacaaccac     2160

cuccaccacg ccaucgacgc ggugaucauc gccuacgcca acaacagcau cgucaaggcc     2220

uucuccgacu ucaagaagga gcaggagagc aacagcgcgg agcuguacgc caagaagauc     2280

uccgagcugg acuacaagaa caagcggaag uucuucgagc ccuucagcgg guuccgccag     2340

aaggugcugg acaagaucga cgagaucuuc guguccaagc ccgagcggaa gaagcccagc     2400

ggcgcccugc acgaggagac cuuccgcaag gaggaggagu ucuaccaguc cuacgggggc     2460

aaggaggggg uccucaaggc ccuggagcug ggcaagaucc ggaaggugaa cggcaagauc     2520

gugaagaacg gggacauguu ccgcgucgac aucuucaagc acaagaagac caacaaguuc     2580

uacgccgugc cgaucuacac gauggacuuc gcgcucaagg ugcugcccaa caaggccguc     2640

gcccggagca agaagggcga gaucaaggac uggauccuga uggacgagaa cuacgaguuc     2700

ugcuucuccc ucuacaagga cagccugauc cugauccaga ccaaggacau gcaggagccc     2760

gaguucgugu acuacaacgc cuucaccucc agcaccgugu cccucaucgu cagcaagcac     2820

gacaacaagu ucgagacgcu guccaagaac cagaagaucc uguucaagaa cgccaacgag     2880

aaggagguga ucgccaagag caucgggauc cagaaccuca agguguucga gaaguacauc     2940

gucuccgcgc ugggcgaggu gaccaaggcc gaguuccgcc agcgggagga cuucaagaag     3000

aagcgccccg ccgccaccaa gaaggcgggg caggccaaga agaagaagua auga           3054


<210>  2327
<211>  3465
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2327
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gagcgaccug       60

gugcucggcc uggacaucgg gaucggcucc gugggggucg gcauccugaa caaggugacc      120

ggcgagauca uccacaagaa cagccggauc uuccccgccg cccaggccga gaacaaccuc      180

gugcgccgga ccaaccgcca ggggcggcgc cuggcccggc gcaagaagca ccggcgcguc      240

cggcugaacc gccucuucga ggaguccggc cugaucacgg acuucaccaa gaucagcauc      300

aaccugaacc ccuaccagcu ccgggugaag gggcugaccg acgagcuguc caacgaggag      360

cuguucaucg cgcugaagaa cauggugaag caccgcggca ucagcuaccu ggacgacgcc      420

uccgacgacg ggaacagcuc cgucggcgac uacgcccaga ucgugaagga gaacagcaag      480

cagcucgaga ccaagacgcc cggccagauc cagcuggagc gguaccagac cuacgggcag      540

cugcgcggcg acuucaccgu ggagaaggac gggaagaagc accggcucau caacgucuuc      600

ccgaccuccg ccuaccgcag cgaggcgcug cggauccugc agacgcagca ggaguucaac      660

ccccagauca ccgacgaguu caucaaccgc uaccucgaga uccugaccgg caagcggaag      720

uacuaccacg gccccgggaa cgagaagucc cgcaccgacu acggccgcua ccggacgagc      780

ggggagaccc uggacaacau cuucggcauc cucaucggga agugcaccuu cuaccccgac      840

gaguuccgcg ccgccaaggc cuccuacacc gcccaggagu ucaaccugcu gaacgaccuc      900

aacaaccuga cggugccgac cgagaccaag aagcugagca aggagcagaa gaaccagauc      960

aucaacuacg ugaagaacga gaaggcgaug ggccccgcca agcuguucaa guacaucgcc     1020

aagcuccugu ccugcgacgu cgccgacauc aagggcuacc ggaucgacaa gagcgggaag     1080

gccgagaucc acaccuucga ggcguaccgc aagaugaaga cgcucgagac ccuggacauc     1140

gagcagaugg accgggagac ccuggacaag cucgccuacg ugcugacccu gaacacggag     1200

cgcgagggca uccaggaggc ccucgagcac gaguucgccg acggguccuu cagccagaag     1260

caggucgacg agcuggugca guuccggaag gccaacagcu ccaucuucgg caaggggugg     1320

cacaacuuca gcgugaagcu gaugauggag cucauccccg agcuguacga gaccuccgag     1380

gagcagauga ccauccugac ccgccucggc aagcagaaga cgaccagcuc cagcaacaag     1440

accaaguaca ucgacgagaa gcugcugacc gaggagaucu acaaccccgu ggucgcgaag     1500

uccgugcggc aggccaucaa gaucgugaac gccgccauca aggaguacgg cgacuucgac     1560

aacaucguca ucgagauggc ccgcgagacg aacgaggacg acgagaagaa ggcgauccag     1620

aagauccaga aggccaacaa ggacgagaag gacgccgcca ugcucaaggc ggccaaccag     1680

uacaacggga aggccgagcu gccgcacagc guguuccacg gccacaagca gcuggccacc     1740

aagauccggc uguggcacca gcagggggag cgcugccucu acaccggcaa gaccaucucc     1800

auccacgacc ugaucaacaa cagcaaccag uucgaggucg accacauccu gccccucucc     1860

aucacguucg acgacagccu ggccaacaag gugcuggugu acgcgaccgc caaccaggag     1920

aaggggcagc ggacccccua ccaggcccuc gacuccaugg acgacgccug gagcuuccgc     1980

gagcugaagg ccuucguccg ggaguccaag acccugagca acaagaagaa ggaguaccuc     2040

cugaccgagg aggacaucuc caaguucgac gugcgcaaga aguucaucga gcggaaccug     2100

guggacacgc gcuacgcgag ccgggucgug cucaacgccc ugcaggagca cuuccgcgcc     2160

cacaagaucg acaccaaggu guccgucgug cgcggccagu ucaccagcca gcugcggcgc     2220

cacuggggca ucgagaagac ccgggacacg uaccaccacc acgccgugga cgcccucauc     2280

aucgcggcca gcucccagcu gaaccugugg aagaagcaga agaacacccu cgucagcuac     2340

uccgaggacc agcugcugga caucgagacc ggggagcuca ucagcgacga cgaguacaag     2400

gaguccgugu ucaaggcccc cuaccagcac uucguggaca cccucaagag caaggaguuc     2460

gaggacucca uccuguucag cuaccagguc gacuccaagu ucaaccgcaa gaucagcgac     2520

gccacgaucu acgcgacccg gcaggccaag gugggcaagg acaaggccga cgagaccuac     2580

gugcucggga agaucaagga caucuacacc caggacggcu acgacgccuu caugaagauc     2640

uacaagaagg acaaguccaa guuccugaug uaccgccacg acccgcagac guucgagaag     2700

gucaucgagc ccauccugga gaacuacccc aacaagcaga ucaacgagaa gggcaaggag     2760

gugcccugca acccguuccu caaguacaag gaggagcacg gguacauccg gaaguacagc     2820

aagaagggca acgggcccga gaucaagucc cugaaguacu acgacagcaa gcugggcaac     2880

cacaucgaca ucacccccaa ggacuccaac aacaaggugg uccuccagag cguguccccc     2940

uggcgcgccg acguguacuu caacaagacc accgggaagu acgagauccu gggccugaag     3000

uacgcggacc uccaguucga gaagggcacg gggaccuaca agaucagcca ggagaaguac     3060

aacgacauca agaagaagga gggcgucgac agcgacuccg aguucaaguu cacccuguac     3120

aagaacgacc ugcuccuggu gaaggacacc gagacgaagg agcagcagcu guuccgguuc     3180

cucagccgca ccaugccgaa gcagaagcac uacguggagc ugaagcccua cgacaagcag     3240

aaguucgagg gcggggaggc ccugaucaag guccucggga acguggccaa cuccggccag     3300

ugcaagaagg gccugggcaa gagcaacauc uccaucuaca aggugcggac cgacguccug     3360

gggaaccagc acaucaucaa gaacgagggg gacaagccca agcucgacuu caagcgcccc     3420

gccgccacca agaaggcggg ccaggccaag aagaagaagu aauga                     3465


<210>  2328
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2328
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gacccaguuc       60

gagggcuuca ccaaccugua ccaggugagc aagacgcucc gguucgagcu gaucccccag      120

gggaagaccc ugaagcacau ccaggagcag ggcuucaucg aggaggacaa ggcccgcaac      180

gaccacuaca aggagcucaa gcccaucauc gaccggaucu acaagaccua cgccgaccag      240

ugccugcagc uggugcagcu cgacugggag aaccuguccg ccgccaucga cagcuaccgc      300

aaggagaaga ccgaggagac gcggaacgcg cugaucgagg agcaggccac cuaccgcaac      360

gccauccacg acuacuucau cgggcggacc gacaaccuca ccgacgccau caacaagcgc      420

cacgcggaga ucuacaaggg ccuguucaag gccgagcugu ucaacggcaa gguccucaag      480

cagcugggga cggugaccac caccgagcac gagaacgccc ugcuccgguc cuucgacaag      540

uucacgaccu acuucagcgg cuucuacgag aaccgcaaga acguguucuc cgccgaggac      600

aucagcaccg ccauccccca ccggaucguc caggacaacu ucccgaaguu caaggagaac      660

ugccacaucu ucacccgccu gaucacggcg gugcccuccc ugcgggagca cuucgagaac      720

gugaagaagg ccaucgggau cuucgucagc accuccaucg aggagguguu cagcuucccc      780

uucuacaacc agcuccugac ccagacccag aucgaccugu acaaccagcu ccugggcggg      840

aucucccgcg aggccggcac ggagaagauc aagggccuga acgaggugcu caaccuggcc      900

auccagaaga acgacgagac cgcccacauc aucgcgagcc ugccccaccg guucaucccc      960

cucuucaagc agauccuguc cgaccgcaac acccugagcu ucauccucga ggaguucaag     1020

uccgacgagg aggucaucca gagcuucugc aaguacaaga cccugcugcg gaacgagaac     1080

gugcucgaga cggccgaggc ccuguucaac gagcugaacu ccaucgaccu cacccacauc     1140

uucaucagcc acaagaagcu ggagaccauc uccagcgccc ugugcgacca cugggacacc     1200

cuccgcaacg cccuguacga gcggcgcauc agcgagcuga cggggaagau caccaagucc     1260

gcgaaggaga aggugcagcg gagccucaag cacgaggaca ucaaccugca ggagaucauc     1320

uccgccgccg gcaaggagcu gagcgaggcc uucaagcaga agaccuccga gauccucagc     1380

cacgcccacg cggcccugga ccagccgcug cccaccacgc ucaagaagca ggaggagaag     1440

gagauccuga agucccagcu ggacagccug cucgggcugu accaccuccu cgacugguuc     1500

gccgucgacg aguccaacga gguggacccc gaguucagcg cccgccugac cggcaucaag     1560

cuggagaugg agccgucccu cagcuucuac aacaaggcgc gcaacuacgc caccaagaag     1620

cccuacuccg uggagaaguu caagcugaac uuccagaugc ccacccuggc cagcggcugg     1680

gacgucaaca aggagaagaa caacggggcc auccucuucg ugaagaacgg ccuguacuac     1740

cuggggauca ugcccaagca gaagggccgg uacaaggccc ucuccuucga gccgacggag     1800

aagaccagcg agggguucga caagauguac uacgacuacu uccccgacgc ggccaagaug     1860

auccccaagu gcuccaccca gcugaaggcc gugaccgccc acuuccagac ccacacgacc     1920

cccauccugc ucagcaacaa cuucaucgag ccgcuggaga ucaccaagga gaucuacgac     1980

cugaacaacc ccgagaagga gcccaagaag uuccagaccg ccuacgcgaa gaagacgggc     2040

gaccagaagg gcuaccgcga ggcccucugc aaguggaucg acuucacccg ggacuuccug     2100

uccaaguaca ccaagaccac gagcaucgac cugagcuccc uccgccccag cucccaguac     2160

aaggaccugg gggaguacua cgccgagcug aacccgcucc uguaccacau cagcuuccag     2220

cggaucgccg agaaggagau cauggacgcc gucgagaccg gcaagcugua ccucuuccag     2280

aucuacaaca aggacuucgc gaaggggcac cacggcaagc ccaaccugca cacccuguac     2340

uggaccggcc ucuucucccc cgagaaccug gccaagacga gcaucaagcu gaacgggcag     2400

gccgagcugu ucuaccgccc caagucccgg augaagcgca uggcccaccg gcugggcgag     2460

aagaugcuga acaagaagcu caaggaccag aagaccccga uccccgacac ccuguaccag     2520

gagcuguacg acuacgugaa ccaccgccuc agccacgacc uguccgacga ggcgcgggcc     2580

cugcucccca acgugaucac caaggagguc agccacgaga ucaucaagga ccgccgguuc     2640

acguccgaca aguucuucuu ccacgugccg aucacccuga acuaccaggc cgccaacagc     2700

cccuccaagu ucaaccagcg cgucaacgcc uaccugaagg agcaccccga gacccccauc     2760

aucgggaucg accggggcga gcgcaaccuc aucuacauca ccgugaucga cagcacgggg     2820

aagauccugg agcagcgguc ccugaacacc auccagcagu ucgacuacca gaagaagcuc     2880

gacaaccgcg agaaggagcg gguggcggcc cgccaggccu ggagcgucgu gggcaccauc     2940

aaggaccuga agcaggggua ccugucccag gugauccacg agaucgucga ccucaugauc     3000

cacuaccagg ccgugguggu ccuggagaac cugaacuucg gcuucaagag caagcgcacc     3060

ggcaucgccg agaaggcggu guaccagcag uucgagaaga ugcucaucga caagcugaac     3120

ugccuggugc ucaaggacua cccggccgag aaggucgggg gcgugcugaa ccccuaccag     3180

cugacggacc aguucaccag cuucgccaag auggggaccc aguccggcuu ccucuucuac     3240

gugcccgccc ccuacaccag caagaucgac ccgcugacgg gcuucgucga ccccuucgug     3300

uggaagacca ucaagaacca cgagucccgg aagcacuucc uggagggguu cgacuuccuc     3360

cacuacgacg ugaagaccgg cgacuucauc cugcacuuca agaugaaccg caaccugagc     3420

uuccagcggg ggcuccccgg cuucaugccc gccugggaca ucgucuucga gaagaacgag     3480

acccaguucg acgcgaaggg gacgccguuc aucgccggca agcgcaucgu gcccgugauc     3540

gagaaccacc gguucaccgg ccgcuaccgg gaccuguacc ccgccaacga gcugaucgcc     3600

cuccuggagg agaaggggau cgucuuccgc gacggcucca acauccugcc caagcuccug     3660

gagaacgacg acagccacgc gaucgacacc augguggccc ugauccgguc cgugcuccag     3720

augcgcaaca gcaacgccgc caccggggag gacuacauca acuccccggu ccgggaccug     3780

aacggcgugu gcuucgacag ccgcuuccag aaccccgagu ggcccaugga cgccgacgcg     3840

aacggggccu accacaucgc ccugaagggc cagcuccugc ugaaccaccu caaggagucc     3900

aaggaccuga agcugcagaa cggcaucagc aaccaggacu ggcucgccua cauccaggag     3960

cugcggaaca agcgccccgc cgcgacgaag aaggccgggc aggccaagaa gaagaaguaa     4020

uga                                                                   4023


<210>  2329
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2329
auggccccca agaagaagcg caaggugggc auccacgggg ucccggccgc gagcaucuac       60

caggaguucg ugaacaagua cucccugagc aagacccucc gguucgagcu gaucccccag      120

ggcaagaccc uggagaacau caaggcccgc gggcucaucc uggacgacga gaagcgggcc      180

aaggacuaca agaaggccaa gcagaucauc gacaaguacc accaguucuu caucgaggag      240

auccugucca gcgugugcau cuccgaggac cuccugcaga acuacagcga cgucuacuuc      300

aagcugaaga aguccgacga cgacaaccuc cagaaggacu ucaagagcgc caaggacacg      360

aucaagaagc agaucuccga guacaucaag gacagcgaga aguucaagaa ccuguucaac      420

cagaaccuga ucgacgcgaa gaagggccag gaguccgacc ucauccugug gcugaagcag      480

agcaaggaca acgggaucga gcuguucaag gccaacuccg acaucaccga caucgacgag      540

gcccucgaga ucaucaagag cuucaagggc uggaccaccu acuucaaggg cuuccacgag      600

aaccgcaaga acguguacuc cagcaacgac auccccacgu ccaucaucua ccggaucgug      660

gacgacaacc ugcccaaguu ccucgagaac aaggccaagu acgagagccu gaaggacaag      720

gcgccggagg ccaucaacua cgagcagauc aagaaggacc uggccgagga gcucaccuuc      780

gacaucgacu acaagaccuc cgaggucaac cagcgcgugu ucagccugga cgagguguuc      840

gagaucgcca acuucaacaa cuaccugaac cagagcggga ucaccaaguu caacacgauc      900

aucggcggga aguucgucaa cggcgagaac accaagcgga aggggaucaa cgaguacauc      960

aaccucuacu cccagcagau caacgacaag acccugaaga aguacaagau gagcgugcug     1020

uucaagcaga uccucuccga caccgagagc aaguccuucg ugaucgacaa gcuggaggac     1080

gacagcgacg ucgugacgac caugcagucc uucuacgagc agaucgccgc guucaagacc     1140

guggaggaga aguccaucaa ggagacccug ucccuccugu ucgacgaccu gaaggcccag     1200

aagcucgacc ugagcaagau cuacuucaag aacgacaagu cccugacgga ccucagccag     1260

caggucuucg acgacuacuc cgugaucggc accgccgugc uggaguacau cacccagcag     1320

aucgccccca agaaccugga caaccccagc aagaaggagc aggagcucau cgccaagaag     1380

accgagaagg cgaaguaccu gucccuggag acgaucaagc ucgcccugga ggaguucaac     1440

aagcaccgcg acaucgacaa gcagugccgg uucgaggaga uccuggccaa cuucgccgcc     1500

auccccauga ucuucgacga gaucgcgcag aacaaggaca accucgccca gaucagcauc     1560

aaguaccaga accagggcaa gaaggaccug cugcaggccu ccgccgagga cgacgucaag     1620

gccaucaagg accuccugga ccagaccaac aaccugcugc acaagcucaa gaucuuccac     1680

aucagccagu ccgaggacaa ggcgaacauc cuggacaagg acgagcacuu cuaccuggug     1740

uucgaggagu gcuacuucga gcucgccaac aucgugccgc uguacaacaa gauccgcaac     1800

uacaucaccc agaagcccua cagcgacgag aaguucaagc ugaacuucga gaacagcacc     1860

cucgccaacg ggugggacaa gaacaaggag cccgacaaca cggccauccu guucaucaag     1920

gacgacaagu acuaccuggg cgucaugaac aagaagaaca acaagaucuu cgacgacaag     1980

gcgaucaagg agaacaaggg ggagggcuac aagaagaucg uguacaagcu ccugcccggc     2040

gccaacaaga ugcugccgaa gguguucuuc uccgccaaga gcaucaaguu cuacaacccc     2100

uccgaggaca uccuccggau ccgcaaccac agcacccaca ccaagaacgg gagcccccag     2160

aagggcuacg agaaguucga guucaacauc gaggacugcc ggaaguucau cgacuucuac     2220

aagcagagca ucuccaagca ccccgagugg aaggacuucg gguuccgcuu cagcgacacc     2280

cagcgguaca acuccaucga cgaguucuac cgcgaggucg agaaccaggg cuacaagcug     2340

acguucgaga acaucagcga guccuacauc gacagcgugg ucaaccaggg gaagcuguac     2400

cucuuccaga ucuacaacaa ggacuucucc gccuacagca agggccggcc gaaccugcac     2460

acccuguacu ggaaggcccu cuucgacgag cgcaaccugc aggacguggu guacaagcug     2520

aacggcgagg cggagcuguu cuaccggaag caguccaucc ccaagaagau cacccacccc     2580

gccaaggagg ccaucgccaa caagaacaag gacaacccca agaaggagag cgucuucgag     2640

uacgaccuca ucaaggacaa gcgcuucacc gaggacaagu ucuucuucca cugcccgauc     2700

acgaucaacu ucaaguccag cggggccaac aaguucaacg acgagaucaa ccugcuccug     2760

aaggagaagg cgaacgacgu gcacauccug agcaucgacc ggggcgagcg ccaccucgcc     2820

uacuacaccc ugguggacgg gaagggcaac aucaucaagc aggacaccuu caacaucauc     2880

gggaacgacc gcaugaagac caacuaccac gacaagcugg ccgccaucga gaaggaccgg     2940

gacuccgccc gcaaggacug gaagaagauc aacaacauca aggagaugaa ggagggcuac     3000

cucagccagg ucgugcacga gaucgcgaag cuggugaucg aguacaacgc caucgucgug     3060

uucgaggacc ugaacuucgg cuucaagcgg gggcgcuuca agguggagaa gcaggucuac     3120

cagaagcucg agaagaugcu gaucgagaag cugaacuacc ucguguucaa ggacaacgag     3180

uucgacaaga cgggcggggu gcugcgggcc uaccagcuga ccgcccccuu cgagaccuuc     3240

aagaagaugg gcaagcagac cgggaucauc uacuacgucc ccgcgggcuu caccuccaag     3300

aucugccccg ugacgggcuu cgugaaccag cucuacccga aguacgagag cgucuccaag     3360

agccaggagu ucuucuccaa guucgacaag aucugcuaca accuggacaa gggguacuuc     3420

gaguucagcu ucgacuacaa gaacuucggc gacaaggccg ccaaggggaa guggaccauc     3480

gccuccuucg gcagccgccu gaucaacuuc cggaacuccg acaagaacca caacugggac     3540

acccgcgagg uguaccccac caaggagcuc gagaagcugc ugaaggacua cagcaucgag     3600

uacggccacg gggagugcau caaggccgcg aucugcggcg aguccgacaa gaaguucuuc     3660

gccaagcuca cgagcgugcu gaacaccauc cugcagaugc ggaacuccaa gaccgggacc     3720

gagcucgacu accugaucag ccccgucgcc gacgugaacg gcaacuucuu cgacucccgc     3780

caggccccca agaacaugcc gcaggacgcc gacgcgaacg gggccuacca caucggccug     3840

aagggccuca ugcugcuggg gcggaucaag aacaaccagg agggcaagaa gcucaaccug     3900

gugaucaaga acgaggagua cuucgaguuc guccagaacc gcaacaacaa gcggcccgcc     3960

gccacgaaga aggccgggca ggcgaagaag aagaaguaau ga                        4002


<210>  2330
<211>  4716
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2330
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgga caagaaguac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccgugaucac cgacgaguac aaggucccca gcaagaaguu caaggugcug gggaacacgg      240

accggcacuc caucaagaag aaccugaucg gcgcccugcu guucgacagc ggcgagaccg      300

ccgaggccac ccgccugaag cggaccgcgc gccggcgcua cacgcggcgc aagaaccgga      360

ucugcuaccu gcaggagauc uucuccaacg agauggccaa gguggacgac agcuucuucc      420

accgccucga ggaguccuuc cuggucgagg aggacaagaa gcacgagcgg caccccaucu      480

ucgggaacau cguggacgag guggccuacc acgagaagua ccccaccauc uaccaccucc      540

gcaagaagcu cgucgacagc accgacaagg ccgaccugcg gcugaucuac cucgcgcugg      600

cccacaugau caaguuccgc ggccacuucc ugaucgaggg ggaccucaac ccggacaacu      660

ccgacgugga caagcuguuc auccagcugg ugcagaccua caaccagcug uucgaggaga      720

accccaucaa cgccagcggc gucgacgcca aggccauccu guccgcgcgg cugagcaagu      780

cccgccggcu cgagaaccug aucgcccagc ugcccgggga gaagaagaac ggccucuucg      840

gcaaccugau cgcccugagc cucgggcuga cgcccaacuu caaguccaac uucgaccugg      900

ccgaggacgc caagcuccag cugagcaagg acaccuacga cgacgaccug gacaaccucc      960

uggcgcagau cggcgaccag uacgccgacc uguuccucgc cgccaagaac cuguccgacg     1020

ccauccugcu cagcgacauc cugcgcguga acaccgagau caccaaggcg ccgcuguccg     1080

ccagcaugau caagcgguac gacgagcacc accaggaccu cacgcugcug aaggcccucg     1140

ugcgccagca gcugcccgag aaguacaagg agaucuucuu cgaccagagc aagaacgggu     1200

acgccggcua caucgacggc ggggccuccc aggaggaguu cuacaaguuc aucaagccca     1260

uccuggagaa gauggacggc accgaggagc uccuggucaa gcugaaccgg gaggaccugc     1320

uccgcaagca gcgcaccuuc gacaacggga gcauccccca ccagauccac cugggcgagc     1380

ugcacgcgau ccuccggcgc caggaggacu ucuacccguu ccugaaggac aaccgggaga     1440

agaucgagaa gauccugacc uuccgcaucc ccuacuacgu ggggccccuc gcccggggca     1500

acucccgcuu cgccuggaug acgcgcaaga gcgaggagac caucaccccc uggaacuucg     1560

aggagguggu cgacaagggc gccuccgcgc agagcuucau cgagcggaug accaacuucg     1620

acaagaaccu gccgaacgag aaggugcugc ccaagcacuc ccuccuguac gaguacuuca     1680

cgguguacaa cgagcugacc aaggucaagu acgugaccga ggggaugcgg aagcccgccu     1740

uccucagcgg cgagcagaag aaggccaucg uggaccugcu guucaagacc aaccgcaagg     1800

ucacggugaa gcagcucaag gaggacuacu ucaagaagau cgagugcuuc gacuccgucg     1860

agaucagcgg gguggaggac cgguucaacg ccucccuggg caccuaccac gaccugcuca     1920

agaucaucaa ggacaaggac uuccuggaca acgaggagaa cgaggacauc cuggaggaca     1980

ucgugcucac ccugacccug uucgaggacc gcgagaugau cgaggagcgg cucaagacgu     2040

acgcccaccu guucgacgac aaggucauga agcagcugaa gcgccggcgc uacaccgggu     2100

ggggccggcu cagccgcaag cugaucaacg gcauccggga caagcagucc gggaagacca     2160

uccuggacuu ccucaagagc gacggcuucg cgaaccgcaa cuucaugcag cugauccacg     2220

acgacucccu gaccuucaag gaggacaucc agaaggccca ggugagcggg cagggcgacu     2280

cccuccacga gcacaucgcc aaccuggccg ggagccccgc caucaagaag ggcauccugc     2340

agacggugaa ggucguggac gagcucguga aggucauggg ccgccacaag ccggagaaca     2400

ucgugaucga gauggcgcgg gagaaccaga ccacccagaa ggggcagaag aacucccgcg     2460

agcggaugaa gcgcaucgag gagggcauca aggagcuggg gagccagauc cugaaggagc     2520

accccgugga gaacacccag cuccagaacg agaagcugua ccuguacuac cuccagaacg     2580

gccgggacau guacgucgac caggagcugg acaucaaccg ccugagcgac uacgacgugg     2640

accacaucgu gccccagucc uuccucaagg acgacagcau cgacaacaag guccugacgc     2700

gguccgacaa gaaccgcggc aagagcgaca acgugcccuc cgaggaggug gucaagaaga     2760

ugaagaacua cuggcggcag cugcucaacg ccaagcugau cacccagcgc aaguucgaca     2820

accugaccaa ggccgagcgc gggggccuca gcgagcugga caaggccggg uucaucaagc     2880

ggcagcuggu ggagacccgg cagaucacca agcacguggc ccagauccuc gacucccgca     2940

ugaacacgaa guacgacgag aacgacaagc ugauccggga ggucaaggug aucacccuca     3000

agagcaagcu cguguccgac uuccgcaagg acuuccaguu cuacaagguc cgggagauca     3060

acaacuacca ccacgcgcac gacgccuacc ugaacgccgu ggugggcacc gcccugauca     3120

agaaguaccc gaagcucgag agcgaguucg ucuacgggga cuacaaggug uacgacgugc     3180

gcaagaugau cgcgaagucc gagcaggaga ucggcaaggc caccgccaag uacuucuucu     3240

acagcaacau caugaacuuc uucaagacgg agaucacccu ggccaacggc gagauccgga     3300

agcgcccccu gaucgagacc aacggggaga ccggcgagau cgucugggac aaggggcggg     3360

acuucgccac ggugcgcaag gugcucucca ugccccaggu caacaucgug aagaagaccg     3420

aggugcagac cggcggguuc agcaaggagu ccauccugcc caagcgcaac agcgacaagc     3480

ugaucgcgcg gaagaaggac ugggacccga agaaguacgg cggcuucgac ucccccaccg     3540

ucgccuacag cgugcucgug gucgccaagg uggagaaggg caagagcaag aagcugaagu     3600

ccgugaagga gcugcucggg aucacgauca uggagcgcag cuccuucgag aagaacccca     3660

ucgacuuccu ggaggccaag ggguacaagg aggucaagaa ggaccugauc aucaagcucc     3720

cgaaguacag ccuguucgag cuggagaacg gccggaagcg caugcucgcc uccgcggggg     3780

agcugcagaa gggcaacgag cuggcccucc ccagcaagua cgugaacuuc cuguaccugg     3840

ccucccacua cgagaagcuc aagggcagcc ccgaggacaa cgagcagaag cagcuguucg     3900

uggagcagca caagcacuac cuggacgaga ucaucgagca gaucuccgag uucagcaagc     3960

gggucauccu cgccgacgcc aaccuggaca aggugcuguc cgcguacaac aagcaccgcg     4020

acaagcccau ccgggagcag gccgagaaca ucauccaccu cuucacccug accaaccugg     4080

gggccccggc cgcguucaag uacuucgaca ccacgaucga ccgcaagcgg uacaccagca     4140

ccaaggaggu gcucgacgcc acccugaucc accaguccau cacgggccug uacgagaccc     4200

gcaucgaccu cagccagcuc gggggcgaca agcggcccgc cgccaccaag aaggccgggc     4260

aggcgaagaa gaagaaguaa ggacuaguga agggaacacc caaauuuaau ucagccuuaa     4320

gcacaauuaa uuaagaguga aacguaauug uacaagcagu uggucaccca ccauagggca     4380

ugaucaacac cgcaaccuuu ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu     4440

cagcuugcuu agauguucca aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa     4500

aaaaaggcca caaaaguucc cucucacuuu caguaaauaa aauaaaagca gcaacagaaa     4560

uaaagaaaua aaugaaauuc aaaaugaaau aaauauugug uugugcagca uuaaaaaauc     4620

aauaaaaauu aaaaaugagc aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               4716


<210>  2331
<211>  5499
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2331
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa cuucaagauc cugcccaucg ccaucgaccu cggcgugaag aacaccgggg      180

uguucagcgc cuucuaccag aagggcaccu cccuggagcg gcuggacaac aagaacggga      240

aggucuacga gcucagcaag gacuccuaca cgcugcugau gaacaaccgc accgcccggc      300

gccaccagcg gcgcggcauc gaccggaagc agcucgugaa gcgccuguuc aagcugaucu      360

ggaccgagca gcucaaccug gagugggaca aggacaccca gcaggccauc agcuuccugu      420

ucaaccggcg cggcuucucc uucaucacgg acggguacag ccccgaguac cucaacaucg      480

ugcccgagca ggucaaggcg auccugaugg acaucuucga cgacuacaac ggcgaggacg      540

accuggacuc cuaccucaag cuggccaccg agcaggagag caagaucucc gagaucuaca      600

acaagcugau gcagaagauc cucgaguuca agcugaugaa gcugugcacc gacaucaagg      660

acgacaaggu gagcaccaag acgcucaagg agaucaccuc cuacgaguuc gagcugcugg      720

ccgacuaccu cgccaacuac agcgaguccc ugaagaccca gaaguucagc uacaccgaca      780

agcaggggaa ccugaaggag cucuccuacu accaccacga caaguacaac auccaggagu      840

uccugaagcg gcacgcgacg aucaacgacc gcauccugga cacccuccug accgacgacc      900

uggacaucug gaacuucaac uucgagaagu ucgacuucga caagaacgag gagaagcucc      960

agaaccagga ggacaaggac cacauccagg cccaccucca ccacuucgug uucgccguca     1020

acaagaucaa gagcgagaug gccuccggcg ggcggcaccg cagccaguac uuccaggaga     1080

ucaccaacgu gcuggacgag aacaaccacc aggagggcua ccugaagaac uucugcgaga     1140

accugcacaa caagaaguac uccaaccuga gcgugaagaa ccucgucaac cugaucggca     1200

accugagcaa ccucgagcug aagccgcugc ggaaguacuu caacgacaag auccacgcca     1260

aggcggacca cugggacgag cagaaguuca cggagaccua cugccacugg auccucgggg     1320

aguggcgcgu gggcgugaag gaccaggaca agaaggacgg ggccaaguac uccuacaagg     1380

accugugcaa cgagcugaag cagaagguca ccaaggccgg ccucguggac uuccugcugg     1440

agcuggaccc cugccggacc aucccgcccu accucgacaa caacaaccgc aagccgccca     1500

agugccagag ccugauccug aaccccaagu uccucgacaa ccaguacccc aacuggcagc     1560

aguaccugca ggagcugaag aagcuccagu ccauccagaa cuaccuggac agcuucgaga     1620

cggaccugaa ggugcucaag uccagcaagg accagcccua cuucgucgag uacaagucca     1680

gcaaccagca gaucgccucc ggccagcggg acuacaagga ccuggacgcc cgcauccugc     1740

aguucaucuu cgaccgggug aaggcgagcg acgagcuccu gcugaacgag aucuacuucc     1800

aggccaagaa gcucaagcag aaggccucca gcgagcugga gaagcuggag uccagcaaga     1860

agcucgacga ggugaucgcc aacucccagc ugagccagau ccugaagucc cagcacacca     1920

acgggaucuu cgagcagggc accuuccucc accuggucug caaguacuac aagcagcgcc     1980

agcgcgcccg ggacagccgc cuguacauca ugcccgagua ccgguacgac aagaagcucc     2040

acaaguacaa caacaccggg cgcuucgacg acgacaacca gcugcugacg uacugcaacc     2100

acaagccccg gcagaagcgc uaccagcucc ugaacgaccu ggcgggcgug cuccaggugu     2160

cccccaacuu ccugaaggac aagaucggga gcgacgacga ccuguucauc agcaaguggc     2220

ucgucgagca cauccggggc uucaagaagg ccugcgagga cucccugaag auccagaagg     2280

acaaccgcgg ccugcucaac cacaagauca acaucgcccg gaacaccaag gggaagugcg     2340

agaaggagau cuucaaccug aucugcaaga ucgagggcag cgaggacaag aaggggaacu     2400

acaagcacgg ccuggccuac gagcucgggg ugcugcuguu cggcgagccg aacgaggccu     2460

ccaagcccga guucgaccgc aagaucaaga aguucaacag caucuacucc uucgcgcaga     2520

uccagcagau cgccuucgcc gagcggaagg gcaacgccaa cacgugcgcg gucugcagcg     2580

ccgacaacgc ccaccgcaug cagcagauca agaucaccga gcccguggag gacaacaagg     2640

acaagaucau ccucuccgcc aaggcccagc ggcugcccgc gaucccgacc cgcaucgugg     2700

acggggccgu caagaagaug gccaccaucc uggccaagaa caucguggac gacaacuggc     2760

agaacaucaa gcagguccuc agcgccaagc accagcugca cauccccauc aucaccgagu     2820

ccaacgcguu cgaguucgag cccgcccugg ccgacgugaa gggcaagagc cucaaggacc     2880

ggcgcaagaa ggcccuggag cggaucuccc ccgagaacau cuucaaggac aagaacaacc     2940

gcaucaagga guucgccaag gggaucagcg cguacuccgg cgccaaccug accgacgggg     3000

acuucgacgg cgccaaggag gagcucgacc acaucauccc gcggagccac aagaaguacg     3060

gcacgcugaa cgacgaggcc aaccugaucu gcgugacccg cggggacaac aagaacaagg     3120

gcaaccgcau cuucugccuc cgggaccugg cggacaacua caagcugaag caguucgaga     3180

ccaccgacga ccucgagauc gagaagaaga ucgccgacac gaucugggac gccaacaaga     3240

aggacuucaa guucgggaac uaccgcuccu ucaucaaccu gacgccccag gagcagaagg     3300

ccuuccggca cgcccuguuc cucgcggacg agaaccccau caagcaggcc gugauccgcg     3360

ccaucaacaa ccggaaccgc accuucguca acggcaccca gcgguacuuc gccgaggugc     3420

uggccaacaa caucuaccug cgcgcgaaga aggagaaccu caacaccgac aagaucagcu     3480

ucgacuacuu cggcaucccc accaucggga acggccgggg gaucgccgag auccgccagc     3540

uguacgagaa gguggacucc gacauccagg ccuacgccaa gggcgacaag ccgcaggcca     3600

gcuacagcca ccugaucgac gcgaugcucg ccuucugcau cgccgccgac gagcaccgga     3660

acgacggguc caucggccug gagaucgaca agaacuacag ccuguacccc cucgacaaga     3720

acaccggcga ggucuucacc aaggacaucu ucucccagau caagaucacc gacaacgagu     3780

ucagcgacaa gaagcuggug cgcaagaagg cgaucgaggg guucaacacg caccggcaga     3840

ugacccgcga cggcaucuac gccgagaacu accugcccau ccucauccac aaggagcuga     3900

acgaggugcg gaagggguac accuggaaga acuccgagga gaucaagauc uucaagggca     3960

agaaguacga cauccagcag cugaacaacc ucgucuacug ccugaaguuc guggacaagc     4020

cgaucagcau cgacauccag aucuccaccc uggaggagcu ccgcaacauc cugacgacca     4080

acaacaucgc cgccaccgcc gaguacuacu acaucaaccu gaagacccag aagcuccacg     4140

aguacuacau cgagaacuac aacacggcgc ugggguacaa gaaguacagc aaggagaugg     4200

aguuccugcg gucccucgcc uaccgcagcg agcgggugaa gaucaagucc aucgacgacg     4260

ucaagcaggu ccuggacaag gacagcaacu ucaucaucgg caagaucacc cugcccuuca     4320

agaaggagug gcagcgccuc uaccgggagu ggcagaacac caccaucaag gacgacuacg     4380

aguuccugaa guccuucuuc aacgucaaga gcaucacgaa gcugcacaag aaggugcgca     4440

aggacuucuc ccuccccauc agcaccaacg agggcaaguu ccuggugaag cgcaagaccu     4500

gggacaacaa cuucaucuac cagauccuga acgacuccga cagccgggcc gacgggacca     4560

agcccuucau cccggccuuc gacaucucca agaacgagau cguggaggcc aucaucgaca     4620

gcuucacgag caagaacauc uucuggcucc ccaagaacau cgagcugcag aagguggaca     4680

acaagaacau cuucgcgauc gacaccucca agugguucga gguggagacc cccagcgacc     4740

ugcgcgacau cggcaucgcc accauccagu acaagaucga caacaacucc cggcccaagg     4800

ugcgcgucaa gcuggacuac gugaucgacg acgacagcaa gaucaacuac uucaugaacc     4860

acucccuccu caagagccgg uacccggaca aggugcugga gauccugaag caguccacga     4920

ucaucgaguu cgagagcucc ggguucaaca agaccaucaa ggagaugcug ggcaugaagc     4980

uggccgggau cuacaacgag accagcaaca acaagcgccc cgccgccacc aagaaggcgg     5040

gccaggccaa gaagaagaag uaaggacuag ugaagggaac acccaaauuu aauucagccu     5100

uaagcacaau uaauuaagag ugaaacguaa uuguacaagc aguuggucac ccaccauagg     5160

gcaugaucaa caccgcaacc uuuccuuuuu cccccaguga uucugaaaaa ccccucuucc     5220

cuucagcuug cuuagauguu ccaaauuuag uaagcuuaag gcggccuaca gaagaaaaag     5280

aaaaaaaagg ccacaaaagu ucccucucac uuucaguaaa uaaaauaaaa gcagcaacag     5340

aaauaaagaa auaaaugaaa uucaaaauga aauaaauauu guguugugca gcauuaaaaa     5400

aucaauaaaa auuaaaaaug agcaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5460

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            5499


<210>  2332
<211>  3771
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2332
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gcggaacuac auccugggcc ucgacaucgg gaucaccagc gugggcuacg      180

ggaucaucga cuacgagacc cgcgacguga ucgacgccgg cguccggcug uucaaggagg      240

ccaacgugga gaacaacgag ggccgccggu ccaagcgcgg ggcccggcgc cugaagcggc      300

gccggcgcca ccggauccag cgcgugaaga agcuccuguu cgacuacaac cugcucacgg      360

accacagcga gcuguccggc aucaaccccu acgaggcccg ggucaagggg cugagccaga      420

agcucuccga ggaggaguuc agcgcggccc ugcugcaccu cgccaagcgc cggggcgugc      480

acaacgugaa cgaggucgag gaggacaccg ggaacgagcu guccaccaag gagcagauca      540

gccgcaacuc caaggcccug gaggagaagu acguggcgga gcuccagcug gagcggcuga      600

agaaggacgg cgaggugcgc ggcagcauca accgguucaa gaccuccgac uacgucaagg      660

aggccaagca gcuccugaag gugcagaagg ccuaccacca gcuggaccag agcuucaucg      720

acacguacau cgaccuccug gagacccgcc gcaccuacua cgaggggccc ggcgaggggu      780

cccccuucgg cuggaaggac aucaaggagu gguacgagau gcugaugggc cacugcaccu      840

acuucccgga ggagcuccgg agcgugaagu acgccuacaa cgccgaccug uacaacgcgc      900

ugaacgaccu caacaaccug gucaucacgc gcgacgagaa cgagaagcug gaguacuacg      960

agaaguucca gaucaucgag aacguguuca agcagaagaa gaagcccacc cucaagcaga     1020

ucgccaagga gauccuggug aacgaggagg acaucaaggg guaccggguc accuccaccg     1080

gcaagcccga guucacgaac cugaaggugu accacgacau caaggacauc accgcccgca     1140

aggagaucau cgagaacgcc gagcuccugg accagaucgc caagauccug accaucuacc     1200

agagcuccga ggacauccag gaggagcuca ccaaccugaa cagcgagcug acgcaggagg     1260

agaucgagca gaucuccaac cucaaggggu acaccggcac ccacaaccug agccugaagg     1320

cgaucaaccu cauccuggac gagcuguggc acaccaacga caaccagauc gccaucuuca     1380

accggcucaa gcuggugccc aagaaggucg accugagcca gcagaaggag aucccgacga     1440

cccucgugga cgacuucauc cugucccccg uggucaagcg cagcuucauc caguccauca     1500

aggugaucaa cgccaucauc aagaaguacg ggcugcccaa cgacaucauc aucgagcugg     1560

cccgggagaa gaacagcaag gacgcccaga agaugaucaa cgagaugcag aagcgcaacc     1620

ggcagaccaa cgagcgcauc gaggagauca uccggaccac gggcaaggag aacgcgaagu     1680

accucaucga gaagaucaag cugcacgaca ugcaggaggg caagugccug uacucccucg     1740

aggccauccc ccuggaggac cugcucaaca acccguucaa cuacgagguc gaccacauca     1800

ucccccgcag cguguccuuc gacaacagcu ucaacaacaa ggugcugguc aagcaggagg     1860

agaacuccaa gaaggggaac cggacccccu uccaguaccu gagcuccagc gacuccaaga     1920

ucagcuacga gaccuucaag aagcacaucc ucaaccuggc caagggcaag gggcgcaucu     1980

ccaagaccaa gaaggaguac cugcucgagg agcgggacau caaccgcuuc agcgugcaga     2040

aggacuucau caaccggaac cugguggaca cgcgcuacgc cacccggggc cugaugaacc     2100

uccugcgcuc cuacuuccgc gucaacaacc uggacgugaa ggucaagagc aucaacgggg     2160

gcuucaccuc cuuccuccgg cgcaagugga aguucaagaa ggagcggaac aagggcuaca     2220

agcaccacgc cgaggacgcg cugaucaucg ccaacgccga cuucaucuuc aaggagugga     2280

agaagcugga caaggccaag aaggugaugg agaaccagau guucgaggag aagcaggcgg     2340

agagcaugcc cgagaucgag accgagcagg aguacaagga gaucuucauc acgccgcacc     2400

agaucaagca caucaaggac uucaaggacu acaaguacag ccaccgcgug gacaagaagc     2460

ccaaccggga gcucaucaac gacacccugu acuccacccg caaggacgac aaggggaaca     2520

cccugaucgu gaacaaccuc aacggccugu acgacaagga caacgacaag cugaagaagc     2580

ucaucaacaa gagccccgag aagcugcuga uguaccacca cgacccccag acguaccaga     2640

agcucaagcu gaucauggag caguacgggg acgagaagaa cccgcuguac aaguacuacg     2700

aggagaccgg caacuaccuc accaaguacu ccaagaagga caacgggccc gucaucaaga     2760

agaucaagua cuacggcaac aagcugaacg cccaccugga caucaccgac gacuacccca     2820

acagccggaa caagguggug aagcucuccc ugaagcccua ccgcuucgac gucuaccugg     2880

acaacggcgu guacaaguuc gugaccguca agaaccucga cgugaucaag aaggagaacu     2940

acuacgaggu gaacagcaag ugcuacgagg aggccaagaa gcugaagaag aucuccaacc     3000

aggccgaguu caucgccagc uucuacaaca acgaccugau caagaucaac ggggagcucu     3060

accgggucau cggcgugaac aacgaccugc ugaaccgcau cgaggugaac augaucgaca     3120

ucacguaccg ggaguaccuc gagaacauga acgacaagcg cccgccccgg aucaucaaga     3180

ccaucgcguc caagacccag agcaucaaga aguacuccac cgacauccug gggaaccugu     3240

acgaggucaa gagcaagaag cacccccaga ucaucaagaa gggcaagcgc cccgccgcca     3300

cgaagaaggc cggccaggcc aagaagaaga aguaaggacu agugaaggga acacccaaau     3360

uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa gcaguugguc     3420

acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu gauucugaaa     3480

aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua aggcggccua     3540

cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua aauaaaauaa     3600

aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua uuguguugug     3660

cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              3771


<210>  2333
<211>  4839
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2333
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgcu guucaacaag ugcaucauca ucagcaucaa ccucgacuuc uccaacaagg      180

agaagugcau gaccaagccc uacagcaucg gccuggacau cgggaccaac uccgugggcu      240

gggccgugau cacggacaac uacaaggucc ccagcaagaa gaugaaggug cuggggaaca      300

ccuccaagaa guacaucaag aagaaccucc ugggcgugcu gcuguucgac agcggcauca      360

ccgccgaggg gcggcgccug aagcggaccg cccgccggcg cuacacgcgg cgccggaacc      420

gcauccugua ccuccaggag aucuucucca ccgagauggc cacccuggac gacgcguucu      480

uccagcggcu ggacgacagc uuccucgucc ccgacgacaa gcgcgacucc aaguacccga      540

ucuucggcaa ccugguggag gagaaggugu accacgacga guuccccacc aucuaccacc      600

uccggaagua ccucgccgac agcacgaaga aggccgaccu gcgccugguc uaccucgccc      660

uggcgcacau gaucaaguac cgggggcacu uccugaucga gggcgaguuc aacuccaaga      720

acaacgacau ccagaagaac uuccaggacu uccucgacac cuacaacgcc aucuucgaga      780

gcgaccuguc ccuggagaac agcaagcagc ucgaggagau cgugaaggac aagaucucca      840

agcuggagaa gaaggaccgc auccugaagc uguuccccgg ggagaagaac agcggcaucu      900

ucuccgaguu ccugaagcug aucgugggca accaggccga cuuccggaag ugcuucaacc      960

ucgacgagaa ggccagccug cacuucucca aggagagcua cgacgaggac cuggagaccc     1020

uccuggggua caucggcgac gacuacagcg acgucuuccu gaaggccaag aagcucuacg     1080

acgcgauccu gcuguccggg uuccucaccg ugacggacaa cgagaccgag gcgccccuga     1140

gcuccgccau gaucaagcgc uacaacgagc acaaggagga ccuggcccuc cugaaggagu     1200

acauccggaa caucagccug aagaccuaca acgagguguu caaggacgac accaagaacg     1260

gcuacgccgg cuacaucgac gggaagacga accaggagga cuucuacguc uaccucaaga     1320

accugcuggc cgaguucgag ggcgccgacu acuuccucga gaagaucgac cgcgaggacu     1380

uccugcggaa gcagcgcacc uucgacaacg gguccauccc guaccagauc caccuccagg     1440

agaugcgcgc cauccuggac aagcaggcca aguucuaccc cuuccucgcc aagaacaagg     1500

agcggaucga gaagauccug accuuccgca uccccuacua cgugggcccc cuggcgcggg     1560

ggaacagcga cuucgccugg uccauccgca agcggaacga gaagaucacc ccguggaacu     1620

ucgaggacgu gaucgacaag gagagcuccg ccgaggccuu caucaaccgc augacgagcu     1680

ucgaccucua ccugcccgag gagaaggucc ugcccaagca cucccuccug uacgagaccu     1740

ucaacgugua caacgagcug accaaggugc gguucaucgc cgagagcaug cgcgacuacc     1800

aguuccucga cuccaagcag aagaaggaca ucguccggcu guacuucaag gacaagcgca     1860

aggugaccga caaggacauc aucgaguacc ugcacgcgau cuacggcuac gacggcaucg     1920

agcucaaggg gaucgagaag caguucaaca gcucccugag cacguaccac gaccugcuca     1980

acaucaucaa cgacaaggag uuccuggacg acuccagcaa cgaggccauc aucgaggaga     2040

ucauccacac ccugaccauc uucgaggacc gggagaugau caagcagcgc cucuccaagu     2100

ucgagaacau cuucgacaag agcgugcuga agaagcugag ccggcgccac uacaccggcu     2160

gggggaagcu cuccgccaag cugaucaacg gcauccggga cgagaagucc gggaacacga     2220

uccuggacua ccucaucgac gacggcauca gcaaccgcaa cuucaugcag cugauccacg     2280

acgacgcccu gagcuucaag aagaagaucc agaaggcgca gaucaucggc gacgaggaca     2340

aggggaacau caaggagguc gugaaguccc uccccggcag cccggccauc aagaagggga     2400

uccugcaguc caucaagauc gucgacgagc uggugaaggu gaugggcggg cggaagcccg     2460

agagcaucgu cguggagaug gcccgcgaga accaguacac caaccagggc aaguccaaca     2520

gccagcagcg gcucaagcgc cuggagaagu cccugaagga gcucggcagc aagauccuga     2580

aggagaacau ccccgccaag cuguccaaga ucgacaacaa cgcccuccag aacgaccgcc     2640

uguaccugua cuaccuccag aacgggaagg acauguacac cggcgacgac cuggacaucg     2700

accggcugag caacuacgac aucgaccaca ucauccccca ggcguuccuc aaggacaacu     2760

ccaucgacaa caaggugcug gucagcuccg ccagcaaccg cgggaagucc gacgacuucc     2820

cgagccugga gguggugaag aagcggaaga ccuucuggua ccagcuccuc aagagcaagc     2880

ugaucuccca gcgcaaguuc gacaaccuga cgaaggccga gcggggcggc cugcugcccg     2940

aggacaaggc cggguucauc cagcgccagc ucgucgagac ccggcagauc accaagcacg     3000

uggcccgccu gcuggacgag aaguucaaca acaagaagga cgagaacaac cgggcggugc     3060

gcaccgucaa gaucaucacg cucaagagca cccugguguc ccaguuccgg aaggacuucg     3120

agcuguacaa ggugcgcgag aucaacgacu uccaccacgc ccacgacgcc uaccucaacg     3180

ccgucaucgc cagcgcgcug cugaagaagu accccaagcu cgagcccgag uucguguacg     3240

gcgacuaccc gaaguacaac uccuuccggg agcgcaagag cgccaccgag aagguguacu     3300

ucuacuccaa caucaugaac aucuucaaga aguccaucag ccuggccgac gggcggguca     3360

ucgagcgccc ccugaucgag gugaacgagg agaccggcga gagcgugugg aacaaggagu     3420

ccgaccucgc caccguccgg cgcgugcuga gcuaccccca ggugaacguc gugaagaagg     3480

uggaggagca gaaccacggg cuggaccggg gcaagcccaa gggccucuuc aacgcgaacc     3540

uguccagcaa gccgaagccc aacuccaacg agaaccuggu cggggccaag gaguaccucg     3600

accccaagaa guacggcggg uacgccggca ucagcaacuc cuucgccgug cuggugaagg     3660

ggacgaucga gaagggcgcc aagaagaaga ucaccaacgu ccuggaguuc cagggcauca     3720

gcauccucga ccgcaucaac uaccggaagg acaagcugaa cuuccugcuc gagaaggggu     3780

acaaggacau cgagcugauc aucgagcugc cgaaguacag ccucuucgag cuguccgacg     3840

gcagccgccg caugcuggcg uccauccuca gcaccaacaa caagcgcggg gagauccaca     3900

agggcaacca gaucuuccug ucccagaagu ucgugaagcu gcucuaccac gccaagcgga     3960

ucagcaacac caucaacgag aaccaccgga aguacgugga gaaccacaag aaggaguucg     4020

aggagcuguu cuacuacauc cuggaguuca acgagaacua cgucggggcc aagaagaacg     4080

gcaagcuccu gaacuccgcc uuccagagcu ggcagaacca cuccaucgac gagcugugca     4140

gcuccuucau cggccccacg gggagcgagc gcaagggccu cuucgagcug accucccggg     4200

ggagcgccgc ggacuucgag uuccugggcg ugaagauccc ccgcuaccgg gacuacaccc     4260

ccuccagccu ccugaaggac gccacccuga uccaccaguc cgugacgggg cuguacgaga     4320

cccgcaucga ccucgccaag cugggcgagg gcaagcggcc ggccgccacc aagaaggcgg     4380

ggcaggccaa gaagaagaag uaaggacuag ugaagggaac acccaaauuu aauucagccu     4440

uaagcacaau uaauuaagag ugaaacguaa uuguacaagc aguuggucac ccaccauagg     4500

gcaugaucaa caccgcaacc uuuccuuuuu cccccaguga uucugaaaaa ccccucuucc     4560

cuucagcuug cuuagauguu ccaaauuuag uaagcuuaag gcggccuaca gaagaaaaag     4620

aaaaaaaagg ccacaaaagu ucccucucac uuucaguaaa uaaaauaaaa gcagcaacag     4680

aaauaaagaa auaaaugaaa uucaaaauga aauaaauauu guguugugca gcauuaaaaa     4740

aucaauaaaa auuaaaaaug agcaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            4839


<210>  2334
<211>  3915
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2334
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgug guacgccagc cugauguccg cccaccaccu ccgggugggc aucgacgugg      180

ggacccacag cgucggccug gccacccugc gcguggacga ccacgggacg cccaucgagc      240

uccuguccgc ccugagccac auccacgacu ccggcguggg caaggagggg aagaaggacc      300

acgacacccg gaagaagcuc agcggcaucg cgcgccgggc ccgccggcug cugcaccacc      360

gccggaccca gcuccagcag cuggacgagg uccugcgcga ccucggguuc cccaucccca      420

ccccgggcga guuccuggac cugaacgagc agacggaccc cuaccgggug uggcgcgugc      480

gggcccgccu cgucgaggag aagcugcccg aggagcugcg ggggcccgcc aucuccaugg      540

cggugcgcca caucgcccgg caccgcggcu ggcggaaccc guacagcaag guggaguccc      600

uccugagccc cgccgaggag ucccccuuca ugaaggcccu gcgcgagcgg auccucgcca      660

ccaccggcga gguccuggac gacgggauca cccccggcca ggcgauggcc cagguggccc      720

ugacgcacaa caucagcaug cgcgggccgg agggcauccu cggcaagcug caccaguccg      780

acaacgccaa cgagauccgc aagaucugcg cccggcaggg ggugagcccc gacgucugca      840

agcagcugcu ccgcgcggug uucaaggccg acagcccccg gggcuccgcc gugucccgcg      900

ucgcccccga cccgcugccc gggcagggca gcuuccggcg cgcccccaag ugcgaccccg      960

aguuccagcg guuccgcauc aucuccaucg uggcgaaccu gcggaucagc gagaccaagg     1020

gggagaaccg cccgcucacc gccgacgagc ggcgccacgu ggucaccuuc cugacggagg     1080

acagccaggc cgaccugacc uggguggacg uggccgagaa gcucggcguc caccggcgcg     1140

accugcgggg caccgccgug cacaccgacg acggggagcg cuccgcggcc cggccgccca     1200

ucgacgccac ggaccgcauc augcggcaga ccaagaucag cucccugaag accugguggg     1260

aggaggccga cagcgagcag cgcggcgcga ugauccgcua ccucuacgag gaccccaccg     1320

acuccgagug cgccgagauc aucgccgagc ugcccgagga ggaccaggcc aagcuggaca     1380

gccuccaccu ccccgccggg cgggcggccu acucccgcga gagccugacg gcccuguccg     1440

accacaugcu ggccaccacc gacgaccugc acgaggcccg gaagcgccuc uucggcgucg     1500

acgacagcug ggcgccgccc gccgaggcca ucaacgcccc cguggggaac cccuccgugg     1560

accggacccu gaagaucguc ggccgcuacc ugagcgccgu ggaguccaug uggggcacgc     1620

ccgaggugau ccacgucgag cacgugcggg acggguucac cagcgagcgc auggcggacg     1680

agcgggacaa ggccaaccgc cggcgcuaca acgacaacca ggaggccaug aagaagaucc     1740

agcgggacua cggcaaggag ggguacaucu cccgcggcga caucgugcgg cucgacgccc     1800

uggagcugca ggggugcgcg ugccuguacu gcggcaccac caucggcuac cacacgugcc     1860

agcucgacca caucgucccg caggccgggc ccggcagcaa caaccgccgg gggaaccugg     1920

uggccgugug cgagcgcugc aaccggucca agagcaacac ccccuucgcc gucugggccc     1980

agaagugcgg caucccccac gugggcguga aggaggcgau cgggcgcguc cggggcuggc     2040

gcaagcagac cccgaacacc uccagcgagg accugacgcg ccucaagaag gaggugaucg     2100

cccggcugcg ccggacccag gaggaccccg agaucgacga gcgcagcaug gaguccgugg     2160

ccuggauggc caacgagcug caccaccgga ucgccgcggc cuaccccgag accaccguca     2220

ugguguaccg cgggagcauc acggccgccg cccggaaggc ggccggcauc gacucccgca     2280

ucaaccucau cggggagaag ggccggaagg accgcaucga ccggcgccac cacgccgugg     2340

acgccagcgu cguggcgcug auggaggccu ccguggccaa gacccuggcc gagcggagcu     2400

cccuccgcgg cgagcagcgg cugaccggga aggagcagac cuggaagcag uacaccggca     2460

gcacggucgg ggcccgcgag cacuucgaga uguggcgggg ccacaugcug caccucaccg     2520

agcuguucaa cgagcgccug gcggaggaca agguguacgu gacccagaac auccggcucc     2580

gccuguccga cgggaacgcc cacaccguca accccagcaa gcuggugucc caccgccucg     2640

gcgacggccu gacggugcag cagaucgacc gggccugcac cccggcccug uggugcgccc     2700

ucacccgcga gaaggacuuc gacgagaaga acgggcugcc cgcgcgggag gaccgcgcca     2760

uccgggucca cggccacgag aucaagagcu ccgacuacau ccagguguuc agcaagcgca     2820

agaagaccga cuccgaccgg gacgagacgc ccuucggggc caucgccgug cgcggcgggu     2880

ucgucgagau cggccccagc auccaccacg cccggaucua ccgcguggag ggcaagaagc     2940

cgguguacgc gaugcugcgg gucuucaccc acgaccuccu gucccagcgc cacggggacc     3000

uguucagcgc cgugaucccg ccccagagca ucuccaugcg gugcgccgag cccaagcucc     3060

gcaaggccau caccaccggc aacgccacgu accuggggug gguggucgug ggcgacgagc     3120

uggagaucaa cguggacagc uucaccaagu acgcgaucgg gcgguuccuc gaggacuucc     3180

ccaacaccac ccgcuggcgg aucugcggcu acgacacgaa cuccaagcug acccugaagc     3240

ccaucguccu cgccgccgag ggccuggaga accccagcuc cgccgugaac gagaucgugg     3300

agcugaaggg guggcgcguc gcgaucaacg ugcucaccaa ggugcacccc accgucgugc     3360

ggcgcgacgc ccugggccgg ccgcgcuaca gcucccgcag caaccugccc acguccugga     3420

ccaucgagaa gcggcccgcc gccaccaaga aggccgggca ggcgaagaag aagaaguaag     3480

gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau uaagagugaa     3540

acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc gcaaccuuuc     3600

cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa     3660

auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc     3720

ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa augaaauuca     3780

aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua aaaaugagca     3840

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3900

aaaaaaaaaa gaauu                                                      3915


<210>  2335
<211>  4776
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2335
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgac caagcccuac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccgugacgac cgacaacuac aaggucccca gcaagaagau gaaggugcuc gggaacaccu      240

ccaagaagua caucaagaag aaccugcugg gcgugcuccu guucgacagc ggcaucaccg      300

ccgaggggcg gcgccugaag cggacggccc gccggcgcua cacccggcgc cggaaccgca      360

uccucuaccu gcaggagauc uucuccaccg agauggccac ccuggacgac gcguucuucc      420

agcggcucga cgacagcuuc cugguccccg acgacaagcg cgacuccaag uacccgaucu      480

ucggcaaccu gguggaggag aaggccuacc acgacgaguu ccccacgauc uaccaccucc      540

ggaaguaccu ggccgacagc accaagaagg ccgaccugcg ccucguguac cuggcgcugg      600

cccacaugau caaguaccgg gggcacuucc ucaucgaggg cgaguucaac uccaagaaca      660

acgacaucca gaagaacuuc caggacuucc uggacaccua caacgccauc uucgagagcg      720

accugucccu cgagaacagc aagcagcugg aggagaucgu caaggacaag aucuccaagc      780

uggagaagaa ggaccgcauc cucaagcugu uccccgggga gaagaacagc ggcaucuucu      840

ccgaguuccu gaagcucauc gugggcaacc aggccgacuu ccggaagugc uucaaccugg      900

acgagaaggc cagccugcac uucuccaagg agagcuacga cgaggaccuc gagacccugc      960

ugggguacau cggcgacgac uacuccgacg uguuccucaa ggcgaagaag cuguacgacg     1020

ccauccugcu cagcggguuc cugacgguca ccgacaacga gaccgaggcg ccccugagcu     1080

ccgccaugau caagcgcuac aacgagcaca aggaggaccu cgcccugcug aaggaguaca     1140

uccggaacau cagccucaag accuacaacg agguguucaa ggacgacacg aagaacggcu     1200

acgccggcua caucgacggg aagaccaacc aggaggacuu cuacguguac cugaagaagc     1260

ugcucgccga guucgagggc gccgacuacu uccuggagaa gaucgaccgc gaggacuucc     1320

ugcggaagca gcgcaccuuc gacaacgggu ccaucccgua ccagauccac cuccaggaga     1380

ugcgcgccau ccuggacaag caggccaagu ucuaccccuu ccuggcgaag aacaaggagc     1440

ggaucgagaa gauccugacc uuccgcaucc ccuacuacgu cggcccccuc gcccggggga     1500

acagcgacuu cgccuggucc auccgcaagc ggaacgagaa gaucacgccg uggaacuucg     1560

aggacgugau cgacaaggag agcuccgccg aggccuucau caaccgcaug accagcuucg     1620

accuguaccu gcccgaggag aaggugcucc ccaagcacuc ccugcuguac gagaccuuca     1680

acgucuacaa cgagcucacc aaggugcggu ucaucgcgga gagcaugcgc gacuaccagu     1740

uccuggacuc caagcagaag aaggacaucg ugcggcugua cuucaaggac aagcgcaagg     1800

ucacggacaa ggacaucauc gaguaccucc acgccaucua cggcuacgac ggcaucgagc     1860

ugaaggggau cgagaagcag uucaacagcu cccugagcac cuaccacgac cuccugaaca     1920

ucaucaacga caaggaguuc cuggacgacu ccagcaacga ggccaucauc gaggagauca     1980

uccacacccu caccaucuuc gaggaccggg agaugaucaa gcagcgccug uccaaguucg     2040

agaacaucuu cgacaagagc gugcugaaga agcucucccg gcgccacuac acgggcuggg     2100

ggaagcugag cgccaagcug aucaacggca uccgggacga gaaguccggg aacaccaucc     2160

ucgacuaccu gaucgacgac ggcaucagca accgcaacuu caugcagcug auccacgacg     2220

acgcgcucag cuucaagaag aagauccaga aggcccagau caucggcgac gaggacaagg     2280

ggaacaucaa ggaggugguc aagucccugc ccggcagccc ggccaucaag aaggggaucc     2340

ugcaguccau caagaucgug gacgagcucg ucaaggugau gggcgggcgg aagcccgaga     2400

gcaucguggu cgagauggcc cgcgagaacc aguacaccaa ccagggcaag uccaacagcc     2460

agcagcggcu gaagcgccug gagaaguccc ucaaggagcu gggcagcaag auccugaagg     2520

agaacauccc cgccaagcuc uccaagaucg acaacaacgc gcugcagaac gaccgccugu     2580

accucuacua ccugcagaac gggaaggaca uguacaccgg cgacgaccug gacaucgacc     2640

ggcucagcaa cuacgacauc gaccacauca ucccccaggc cuuccugaag gacaacucca     2700

ucgacaacaa ggugcuggug agcuccgcca gcaaccgcgg gaaguccgac gacgucccga     2760

gccucgaggu ggugaagaag cggaagacgu ucugguacca gcugcucaag agcaagcuga     2820

ucucccagcg caaguucgac aaccugacca aggccgagcg gggcggccug agccccgagg     2880

acaaggccgg guucauccag cgccagcucg ucgagacccg gcagaucacc aagcacgugg     2940

cgcgccugcu ggacgagaag uucaacaaca agaaggacga gaacaaccgg gccgugcgca     3000

cggucaagau caucacccuc aaguccaccc uggugagcca guuccggaag gacuucgagc     3060

uguacaaggu gcgcgagauc aacgacuucc accacgccca cgacgccuac cucaacgccg     3120

ucguggcguc cgcccugcug aagaaguacc ccaagcucga gcccgaguuc guguacggcg     3180

acuacccgaa guacaacagc uuccgggagc gcaaguccgc caccgagaag gucuacuucu     3240

acagcaacau caugaacauc uucaagaagu ccaucagccu ggccgacggg cgggugaucg     3300

agcgcccccu gaucgaggug aacgaggaga ccggcgaguc cgucuggaac aaggagagcg     3360

accucgcgac ggugcggcgc gugcuguccu acccccaggu caacguggug aagaaggucg     3420

aggagcagaa ccacgggcug gaccggggca agcccaaggg ccucuucaac gccaaccuga     3480

gcuccaagcc gaagcccaac agcaacgaga accugguggg ggccaaggag uaccucgacc     3540

ccaagaagua cggcggguac gccggcaucu ccaacagcuu caccgugcug gucaagggga     3600

ccaucgagaa gggcgccaag aagaagauca ccaacgugcu ggaguuccag ggcaucucca     3660

uccucgaccg caucaacuac cggaaggaca agcugaacuu ccugcucgag aagggguaca     3720

aggacaucga gcugaucauc gagcugccga aguacagccu cuucgagcug agcgacggcu     3780

cccgccgcau gcuggcgagc auccucucca cgaacaacaa gcgcggggag auccacaagg     3840

gcaaccagau cuuccugagc cagaaguucg ugaagcugcu cuaccacgcc aagcggaucu     3900

ccaacaccau caacgagaac caccggaagu acgucgagaa ccacaagaag gaguucgagg     3960

agcuguucua cuacauccug gaguucaacg agaacuacgu gggggccaag aagaacggca     4020

agcuccugaa cagcgccuuc caguccuggc agaaccacag caucgacgag cugugcucca     4080

gcuucaucgg ccccaccggg uccgagcgca agggccucuu cgagcugacc agccgggggu     4140

ccgccgcgga cuucgaguuc cugggcguga agaucccccg cuaccgggac uacacgccca     4200

gcucccuccu gaaggacgcc acccugaucc accagagcgu caccgggcuc uacgagaccc     4260

gcaucgaccu ggccaagcug ggcgagggca agcggccggc cgccacgaag aaggcggggc     4320

aggccaagaa gaagaaguaa ggacuaguga agggaacacc caaauuuaau ucagccuuaa     4380

gcacaauuaa uuaagaguga aacguaauug uacaagcagu uggucaccca ccauagggca     4440

ugaucaacac cgcaaccuuu ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu     4500

cagcuugcuu agauguucca aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa     4560

aaaaaggcca caaaaguucc cucucacuuu caguaaauaa aauaaaagca gcaacagaaa     4620

uaaagaaaua aaugaaauuc aaaaugaaau aaauauugug uugugcagca uuaaaaaauc     4680

aauaaaaauu aaaaaugagc aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               4776


<210>  2336
<211>  3858
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2336
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc cgccuucaag cccaacagca ucaacuacau ccugggccuc gacaucggga      180

ucgccuccgu gggcugggcc augguggaga ucgacgagga ggagaacccc auccggcuga      240

ucgaccuggg gguccgcgug uucgagcggg cggaggugcc caagaccggc gacagccucg      300

ccauggcccg ccggcuggcc cgcuccgucc ggcgccugac ccggcgccgg gcgcaccgcc      360

uccugcggac gcgccggcug cucaagcgcg agggcgugcu gcaggccgcc aacuucgacg      420

agaacgggcu gaucaagagc cucccgaaca cccccuggca gcugcgggcc gccgcgcugg      480

accgcaagcu cacgccccug gagugguccg ccgugcugcu ccaccugauc aagcaccggg      540

gcuaccugag ccagcgcaag aacgaggggg agaccgccga caaggagcuc ggcgcccugc      600

ugaagggggu cgccggcaac gcgcacgccc uccagaccgg cgacuuccgg acccccgccg      660

agcuggcccu gaacaaguuc gagaaggagu ccgggcacau ccgcaaccag cgcagcgacu      720

acucccacac cuucagccgg aaggaccucc aggccgagcu gauccuccug uucgagaagc      780

agaaggaguu cggcaacccg cacguguccg ggggccugaa ggagggcauc gagacccugc      840

ucaugacgca gcgccccgcg cugagcgggg acgccgugca gaagaugcug ggccacugca      900

ccuucgagcc cgccgagccc aaggccgcca agaacaccua caccgcggag cgguucaucu      960

ggcucacgaa gcugaacaac cugcgcaucc ucgagcaggg guccgagcgg ccgcugaccg     1020

acaccgagcg cgccacccug auggacgagc ccuaccgcaa gagcaagcuc acguacgccc     1080

aggcccggaa gcugcugggc cucgaggaca ccgcguucuu caaggggcug cgguacggca     1140

aggacaacgc cgaggccucc acccugaugg agaugaaggc cuaccacgcc aucagccgcg     1200

cgcucgagaa ggagggccug aaggacaaga aguccccgcu gaaccugagc cccgagcucc     1260

aggacgagau cgggaccgcc uucucccugu ucaagacgga cgaggacauc accggccggc     1320

ugaaggaccg cauccagccg gagauccucg aggcccugcu gaagcacauc agcuucgaca     1380

aguucgucca gaucagccuc aaggcccugc ggcgcaucgu gccccugaug gagcagggga     1440

agcgguacga cgaggccugc gcggagaucu acggcgacca cuacgggaag aagaacaccg     1500

aggagaagau cuaccucccg cccauccccg ccgacgagau ccgcaacccc gugguccugc     1560

gggcccuguc ccaggcccgc aaggugauca acggcguggu ccggcgcuac ggcagccccg     1620

cccgcaucca caucgagacc gcgcgggagg uggggaaguc cuucaaggac cgcaaggaga     1680

ucgagaagcg gcaggaggag aaccgcaagg accgggagaa ggccgccgcc aaguuccgcg     1740

aguacuuccc caacuucgug ggcgagccca agagcaagga cauccucaag cugcggcugu     1800

acgagcagca gcacgggaag ugccucuacu ccggcaagga gaucaaccug gggcgccuga     1860

acgagaaggg cuacgucgag aucgaccacg cgcuccccuu cagccggacg ugggacgacu     1920

ccuucaacaa caaggugcug gugcugggca gcgagaacca gaacaagggg aaccagaccc     1980

ccuacgagua cuucaacggc aaggacaacu cccgcgagug gcaggaguuc aaggcccggg     2040

ucgagaccag ccgcuucccc cgguccaaga agcagcgcau ccuccugcag aaguucgacg     2100

aggacggguu caaggagcgg aaccugaacg acacccgcua cgugaaccgg uuccucugcc     2160

aguucgucgc cgaccgcaug cggcugacgg gcaagggcaa gaagcgcgug uucgccagca     2220

acgggcagau caccaaccug cuccggggcu ucugggggcu gcgcaaggug cgcgccgaga     2280

acgaccggca ccacgcgcug gacgccgucg ugguggccug cuccaccguc gccaugcagc     2340

agaagaucac ccgcuucgug cgguacaagg agaugaacgc cuucgacggc aagacgaucg     2400

acaaggagac cggggaggug cuccaccaga agacccacuu cccgcagccc ugggaguucu     2460

ucgcgcagga ggucaugauc cgcguguucg gcaagcccga cggcaagccg gaguucgagg     2520

aggccgacac ccuggagaag cugcggacgc uccuggccga gaagcugagc ucccgccccg     2580

aggccgugca cgaguacguc acgccccucu ucgugagccg ggcccccaac cgcaagaugu     2640

ccgggcaggg ccacauggag accgucaaga gcgcgaagcg gcuggacgag gggguguccg     2700

ugcugcgcgu gccgcucacc cagcugaagc ugaaggaccu cgagaagaug gucaaccggg     2760

agcgcgagcc caagcuguac gaggcccuga aggcccggcu cgaggcccac aaggacgacc     2820

ccgcgaaggc cuucgccgag cccuucuaca aguacgacaa ggccggcaac cgcacccagc     2880

aggucaaggc cgugcgggug gagcaggucc agaagaccgg ggugugggug cgcaaccaca     2940

acggcaucgc ggacaacgcc accauggugc ggguggacgu cuucgagaag ggcgacaagu     3000

acuaccuggu gccgaucuac agcuggcagg ucgccaaggg gauccugccc gaccgcgccg     3060

uggugcaggg caaggacgag gaggacuggc agcucaucga cgacagcuuc aacuucaagu     3120

ucucccugca ccccaacgac cugguggagg ucaucaccaa gaaggcccgg auguucgggu     3180

acuucgcgag cugccaccgc ggcaccggga acaucaacau ccgcauccac gaccucgacc     3240

acaagaucgg caagaacggc auccuggagg ggaucggcgu gaagacggcc cuguccuucc     3300

agaaguacca gaucgacgag cucgggaagg agauccggcc cugccgccug aagaagcggc     3360

cgcccgugcg caagcggccc gccgccacca agaaggccgg ccaggcgaag aagaagaagu     3420

aaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu aauuaagagu     3480

gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac accgcaaccu     3540

uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc uuagauguuc     3600

caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu     3660

cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau     3720

ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga     3780

gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaagaauu                                                   3858


<210>  2337
<211>  4614
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2337
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gaagcccuac accaucggcc uggacaucgg gaccaacagc gugggcuggg      180

ccgugcucac ggaccaguac gaccugguca agcggaagau gaagaucgcc ggggacuccg      240

agaagaagca gaucaagaag aacuucuggg gcgugcgccu guucgacgag ggccagaccg      300

ccgccgaccg gcgcauggcg cggaccgccc gccggcgcau cgagcggcgc cggaaccgca      360

ucagcuaccu ccaggggauc uucgccgagg agauguccaa gaccgacgcc aacuucuucu      420

gccggcugag cgacuccuuc uacguggaca acgagaagcg caacagccgg caccccuucu      480

ucgcgacgau cgaggaggag gucgaguacc acaagaacua ccccaccauc uaccaccucc      540

gcgaggagcu ggugaacucc agcgagaagg ccgaccugcg gcugguguac cucgcccugg      600

cccacaucau caaguaccgc ggcaacuucc ugaucgaggg ggcccucgac acccagaaca      660

ccuccgucga cggcaucuac aagcaguuca uccagacgua caaccaggug uucgcgagcg      720

ggaucgagga cggcucccug aagaagcugg aggacaacaa ggacguggcc aagauccucg      780

ucgagaaggu gacccggaag gagaagcugg agcgcauccu gaagcucuac ccgggcgaga      840

aguccgccgg gauguucgcc caguucauca gccugaucgu gggcagcaag gggaacuucc      900

agaagcccuu cgaccugauc gagaaguccg acaucgagug cgccaaggac agcuacgagg      960

aggaccucga gucccugcug gcgcucaucg gcgacgagua cgccgagcug uucgucgccg     1020

ccaagaacgc cuacagcgcg guggugcugu ccagcaucau caccgucgcc gagaccgaga     1080

cgaacgccaa gcucagcgcc uccaugaucg agcgguucga cacccacgag gaggaccugg     1140

gcgagcugaa ggccuucauc aagcuccacc uccccaagca cuacgaggag aucuucagca     1200

acaccgagaa gcacggguac gcgggcuaca ucgacgggaa gaccaagcag gccgacuucu     1260

acaaguacau gaagaugacg cuggagaaca ucgagggcgc cgacuacuuc aucgccaaga     1320

ucgagaagga gaacuuccug cgcaagcagc gcaccuucga caacggggcg aucccccacc     1380

agcugcaccu ggaggagcuc gaggccaucc ugcaccagca ggccaaguac uacccguucc     1440

ugaaggagaa cuacgacaag aucaaguccc ucgugaccuu ccggaucccc uacuucgugg     1500

gcccccuggc caacggccag agcgaguucg ccuggcugac ccgcaaggcg gacggggaga     1560

uccggcccug gaacaucgag gagaaggucg acuucggcaa guccgccgug gacuucaucg     1620

agaagaugac gaacaaggac accuaccucc cgaaggagaa cgugcugccc aagcacagcc     1680

ugugcuacca gaaguaccuc gucuacaacg agcugaccaa ggugcgcuac aucaacgacc     1740

aggggaagac cuccuacuuc agcggccagg agaaggagca gaucuucaac gaccuguuca     1800

agcagaagcg gaaggucaag aagaaggacc ucgagcuguu ccugcgcaac augucccacg     1860

uggagagccc cacgaucgag gggcuggagg acuccuucaa cagcuccuac agcaccuacc     1920

acgaccuccu gaaggugggc aucaagcagg agauccugga caaccccguc aacaccgaga     1980

ugcucgagaa caucgugaag auccugaccg uguucgagga caagcggaug aucaaggagc     2040

agcugcagca guucuccgac guccucgacg gcguggugcu gaagaagcug gagcgccggc     2100

acuacacggg guggggccgc cucagcgcca agcugcugau ggggauccgg gacaagcagu     2160

cccaccucac cauccuggac uaccugauga acgacgacgg ccucaaccgc aaccugaugc     2220

agcugaucaa cgacagcaac cucuccuuca agagcaucau cgagaaggag caggucacca     2280

ccgccgacaa ggacauccag agcaucgugg ccgaccuggc gggguccccg gccaucaaga     2340

agggcauccu gcagagccuc aagaucgugg acgagcuggu cuccgugaug ggcuacccgc     2400

cccagacgau cguggucgag auggcccggg agaaccagac caccgggaag ggcaagaaca     2460

acagccgccc ccgguacaag ucccuggaga aggccaucaa ggaguucggg agccagaucc     2520

ucaaggagca ccccaccgac aaccaggagc ugcgcaacaa ccggcuguac cucuacuacc     2580

ugcagaacgg caaggacaug uacacgggcc aggaccugga cauccacaac cucuccaacu     2640

acgacaucga ccacaucgug ccccagagcu ucaucaccga caacuccauc gacaaccugg     2700

ugcugaccag cuccgccggg aaccgcgaga agggcgacga cgucccgccc cucgagaucg     2760

ugcggaagcg caagguguuc ugggagaagc uguaccaggg gaaccugaug agcaagcgca     2820

aguucgacua ccucaccaag gcggagcggg gcgggcugac cgaggccgac aaggcccgcu     2880

ucauccaccg gcagcugguc gagacgcgcc agaucaccaa gaacguggcc aacauccucc     2940

accagcgguu caacuacgag aaggacgacc acggcaacac caugaagcag guccgcaucg     3000

ugacccugaa guccgcgcug gugagccagu uccggaagca guuccagcuc uacaaggugc     3060

gcgacgucaa cgacuaccac cacgcccacg acgccuaccu gaacggcgug guggccaaca     3120

cgcugcucaa ggucuacccc cagcuggagc ccgaguucgu guacggggac uaccaccagu     3180

ucgacugguu caaggccaac aaggcgaccg ccaagaagca guucuacacc aacaucaugc     3240

uguucuucgc ccagaaggac cggaucaucg acgagaacgg cgagauccuc ugggacaaga     3300

aguaccugga caccgugaag aaggucaugu ccuaccgcca gaugaacauc gugaagaaga     3360

cggagaucca gaagggggag uucagcaagg ccaccaucaa gcccaagggc aacuccagca     3420

agcugauccc ccggaagacc aacugggacc cgaugaagua cgggggccuc gacagcccca     3480

acauggccua cgcggugguc aucgaguacg ccaagggcaa gaacaagcug guguucgaga     3540

agaagaucau ccgcgugacc aucauggagc ggaaggccuu cgagaaggac gagaaggccu     3600

uccuggagga gcagggguac cgccagccca agguccucgc caagcugccg aaguacacgc     3660

uguacgagug cgaggagggc cggcgccgga ugcucgcguc cgccaacgag gcccagaagg     3720

ggaaccagca gguccugccc aaccaccugg ugacccuccu gcaccacgcc gcgaacugcg     3780

aggugagcga cggcaagucc cuggacuaca ucgagagcaa ccgcgagaug uucgccgagc     3840

uccuggccca cguguccgag uucgccaagc gguacacccu ggccgaggcg aaccucaaca     3900

agaucaacca gcuguucgag cagaacaagg agggggacau caaggccauc gcccagagcu     3960

ucguggaccu gauggccuuc aacgccaugg gcgcgcccgc cuccuucaag uucuucgaga     4020

ccacgaucga gcgcaagcgg uacaacaacc ucaaggagcu gcugaacagc accaucaucu     4080

accaguccau caccggccuc uacgagagcc gcaagcgccu ggacgacaag cggcccgccg     4140

ccaccaagaa ggccgggcag gcgaagaaga agaaguaagg acuagugaag ggaacaccca     4200

aauuuaauuc agccuuaagc acaauuaauu aagagugaaa cguaauugua caagcaguug     4260

gucacccacc auagggcaug aucaacaccg caaccuuucc uuuuuccccc agugauucug     4320

aaaaaccccu cuucccuuca gcuugcuuag auguuccaaa uuuaguaagc uuaaggcggc     4380

cuacagaaga aaaagaaaaa aaaggccaca aaaguucccu cucacuuuca guaaauaaaa     4440

uaaaagcagc aacagaaaua aagaaauaaa ugaaauucaa aaugaaauaa auauuguguu     4500

gugcagcauu aaaaaaucaa uaaaaauuaa aaaugagcaa gaucuaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           4614


<210>  2338
<211>  4647
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2338
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gaagcccuac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccguggucac cgacgacuac aaggugcccg ccaagaagau gaaggugcuc gggaacacgg      240

acaagagcca caucgagaag aaccugcugg gcgcccuccu guucgacucc ggcaacaccg      300

ccgaggaccg gcgccugaag cggaccgcgc gccggcgcua cacccggcgc cggaaccgca      360

uccucuaccu gcaggagauc uucagcgagg agauggggaa ggucgacgac uccuucuucc      420

accggcugga ggacagcuuc cucgugacgg aggacaagcg cggcgagcgg caccccaucu      480

ucgggaaccu ggaggaggag gugaaguacc acgagaacuu cccgaccauc uaccaccucc      540

gccaguaccu ggccgacaac cccgagaagg ucgaccugcg gcugguguac cucgcccugg      600

cccacaucau caaguuccgc ggccacuucc ugaucgaggg gaaguucgac acccggaaca      660

acgacgugca gcgccucuuc caggaguucc uggcggucua cgacaacacc uucgagaacu      720

ccagccugca ggagcagaac guccaggugg aggagauccu cacggacaag aucuccaaga      780

gcgccaagaa ggaccgggug cugaagcugu uccccaacga gaaguccaac ggccgcuucg      840

ccgaguuccu caagcugauc gugggcaacc aggccgacuu caagaagcac uucgagcugg      900

aggagaaggc cccgcuccag uucagcaagg acaccuacga ggaggagcug gaggugcugc      960

ucgcgcagau cggggacaac uacgccgagc uguuccuguc cgccaagaag cucuacgaca     1020

gcauccugcu guccggcauc cucaccguca ccgacguggg gacgaaggcc ccgcugagcg     1080

ccuccaugau ccagcgguac aacgagcacc agauggaccu ggcgcagcuc aagcaguuca     1140

uccgccagaa gcugagcgac aaguacaacg agguguucuc cgacgucagc aaggacggcu     1200

acgccggcua caucgacggg aagaccaacc aggaggccuu cuacaaguac cugaagggcc     1260

uccugaacaa gaucgagggg agcggcuacu uccuggacaa gaucgagcgc gaggacuucc     1320

uccggaagca gcgcaccuuc gacaacgggu ccauccccca ccagauccac cuccaggaga     1380

ugcgggccau cauccgccgg caggccgagu ucuaccccuu ccuggcggac aaccaggacc     1440

gcaucgagaa gcugcugacc uuccggaucc ccuacuacgu gggcccgcug gcccgcggca     1500

agagcgacuu cgccuggcug ucccgcaaga gcgccgacaa gaucacgccc uggaacuucg     1560

acgagaucgu ggacaaggag uccagcgccg aggcguucau caaccggaug accaacuacg     1620

accucuaccu gcccaaccag aagguccugc ccaagcacuc ccuccuguac gagaaguuca     1680

ccguguacaa cgagcugacc aaggucaagu acaagacgga gcaggggaag accgccuucu     1740

ucgacgccaa caugaagcag gagaucuucg acggcguguu caagguguac cggaagguca     1800

ccaaggacaa gcucauggac uuccuggaga aggaguucga cgaguuccgc aucguggacc     1860

ugaccgggcu cgacaaggag aacaaggugu ucaacgccag cuacggcacg uaccacgacc     1920

ugugcaagau ccuggacaag gacuuccucg acaacuccaa gaacgagaag auccuggagg     1980

acaucguccu gacccucacc cuguucgagg accgggagau gauccgcaag cggcuggaga     2040

acuacagcga ccuccugacc aaggagcagg ucaagaagcu ggagcgccgg cacuacacgg     2100

gguggggccg ccucuccgcg gagcugaucc acggcauccg gaacaaggag agccgcaaga     2160

ccauccugga cuaccucauc gacgacggga acuccaaccg caacuucaug cagcugauca     2220

acgacgacgc ccugagcuuc aaggaggaga ucgccaaggc ccaggugauc ggcgagaccg     2280

acaaccucaa ccagguggug uccgacaucg ccgggagccc ggcgaucaag aagggcaucc     2340

ugcagucccu gaagaucgug gacgagcucg ucaagaucau ggggcaccag cccgagaaca     2400

ucguggugga gauggcccgg gagaaccagu ucaccaacca gggccgccgg aacagccagc     2460

agcgccugaa gggccugacg gacuccauca aggaguucgg gagccagauc cucaaggagc     2520

accccgucga gaacagccag cugcagaacg accggcuguu ccucuacuac cugcagaacg     2580

gccgcgacau guacaccggg gaggagcugg acaucgacua ccucucccag uacgacaucg     2640

accacaucau cccccaggcc uucaucaagg acaacagcau cgacaaccgg gugcugaccu     2700

ccagcaagga gaaccgcggc aaguccgacg acgugccgag caaggacguc gugcggaaga     2760

ugaaguccua cuggagcaag cugcucuccg ccaagcugau cacccagcgc aaguucgaca     2820

accugacgaa ggccgagcgg ggcgggcuca ccgacgacga caaggcgggc uucaucaagc     2880

gccagcuggu ggagacccgg cagaucacca agcacgucgc ccgcauccug gacgagcggu     2940

ucaacaccga gacggacgag aacaacaaga agauccgcca ggugaagauc gugacccuca     3000

agagcaaccu ggucuccaac uuccggaagg aguucgagcu guacaaggug cgcgagauca     3060

acgacuacca ccacgcccac gacgccuacc ucaacgccgu gaucgggaag gcgcugcugg     3120

gcgucuaccc ccagcucgag cccgaguucg uguacgggga cuacccccac uuccacggcc     3180

acaaggagaa caaggccacc gccaagaagu ucuucuacag caacaucaug aacuucuuca     3240

agaaggacga cgugcggacc gacaagaacg gcgagaucau cuggaagaag gacgagcaca     3300

ucuccaacau caagaagguc cugagcuacc cccaggugaa caucgugaag aaggucgagg     3360

agcagaccgg gggcuucucc aaggagagca uccugcccaa ggggaacucc gacaagcuca     3420

ucccccgcaa gacgaagaag uucuacuggg acaccaagaa guacggcggg uucgacagcc     3480

ccaucguggc cuacagcauc cuggugaucg cggacaucga gaagggcaag uccaagaagc     3540

ugaagaccgu caaggcccuc gugggcguga cgaucaugga gaagaugacc uucgagcggg     3600

acccggucgc cuuccuggag cgcaaggggu accgcaacgu gcaggaggag aacaucauca     3660

agcugcccaa guacagccuc uucaagcugg agaacggccg gaagcgccug cucgccuccg     3720

cccgggagcu gcagaagggg aacgagaucg ugcugcccaa ccaccucggc acccugcugu     3780

accacgcgaa gaacauccac aaggucgacg agccgaagca ccucgacuac guggacaagc     3840

acaaggacga guucaaggag cugcuggacg uggucagcaa cuucuccaag aaguacaccc     3900

ucgccgaggg gaaccuggag aagaucaagg agcuguacgc ccagaacaac ggcgaggacc     3960

ucaaggagcu ggccagcucc uucaucaacc ugcucacguu caccgccauc ggcgcgcccg     4020

ccaccuucaa guucuucgac aagaacaucg accgcaagcg guacaccagc acgaccgaga     4080

uccugaacgc cacccugauc caccagucca ucaccgggcu cuacgagacg cgcaucgacc     4140

ugaacaagcu gggcggggac aagcggcccg ccgccaccaa gaaggcgggc caggccaaga     4200

agaagaagua aggacuagug aagggaacac ccaaauuuaa uucagccuua agcacaauua     4260

auuaagagug aaacguaauu guacaagcag uuggucaccc accauagggc augaucaaca     4320

ccgcaaccuu uccuuuuucc cccagugauu cugaaaaacc ccucuucccu ucagcuugcu     4380

uagauguucc aaauuuagua agcuuaaggc ggccuacaga agaaaaagaa aaaaaaggcc     4440

acaaaaguuc ccucucacuu ucaguaaaua aaauaaaagc agcaacagaa auaaagaaau     4500

aaaugaaauu caaaaugaaa uaaauauugu guugugcagc auuaaaaaau caauaaaaau     4560

uaaaaaugag caagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaaaaaa aagaauu                                         4647


<210>  2339
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2339
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgca gaccaccaac cugagcuaca uccucggccu ggaccugggg aucgccuccg      180

ugggcugggc cguggucgag aucaacgaga acgaggaccc caucgggcuc aucgacgugg      240

gcgugcggau cuucgagcgc gccgaggucc ccaagacggg cgagagccug gcccuguccc      300

ggcgccucgc gcggagcacc cgccggcuga uccgccggcg cgcccaccgg cugcuccugg      360

ccaagcgcuu ccugaagcgg gaggggaucc ucuccaccau cgaccuggag aagggccugc      420

ccaaccaggc cugggagcuc cgcguggcgg ggcuggagcg gcgccugagc gccaucgagu      480

ggggcgccgu gcuccugcac cugaucaagc accgggggua ccucuccaag cgcaagaacg      540

agagccagac caacaacaag gagcugggcg cccugcucuc cggcgucgcc cagaaccacc      600

agcugcugca gagcgacgac uaccggacgc cggcggagcu cgcccugaag aaguucgcca      660

aggaggaggg gcacauccgc aaccagcggg gcgccuacac ccacaccuuc aaccgccugg      720

accuccuggc cgagcugaac cuccuguucg cgcagcagca ccaguucggg aacccccacu      780

gcaaggagca cauccagcag uacaugaccg agcugcucau guggcagaag cccgcccugu      840

ccggcgaggc cauccugaag augcucggca agugcacgca cgagaagaac gaguucaagg      900

ccgccaagca caccuacagc gcggagcgcu ucguguggcu gaccaagcug aacaaccucc      960

ggauccugga ggacggggcc gagcgcgccc ugaacgagga ggagcggcag cuccugauca     1020

accaccccua cgagaagucc aagcugaccu acgcccaggu gcgcaagcuc cugggccuga     1080

gcgagcaggc caucuucaag caccuccggu acuccaagga gaacgcggag agcgccacgu     1140

ucauggagcu caaggccugg cacgccaucc gcaaggcgcu ggagaaccag gggcugaagg     1200

acaccuggca ggaccucgcc aagaagccgg accugcugga cgagaucggc accgccuucu     1260

cccucuacaa gaccgacgag gacauccagc aguaccugac gaacaagguc cccaacagcg     1320

ugaucaacgc ccugcucgug ucccugaacu ucgacaaguu caucgagcug agccucaaga     1380

gccugcggaa gauccugccc cucauggagc aggggaagcg cuacgaccag gccugccggg     1440

agaucuacgg ccaccacuac ggcgaggcga accagaagac cucccagcug cugcccgcca     1500

ucccggccca ggagauccgc aaccccgucg ugcuccggac ccugagccag gcccgcaagg     1560

ugaucaacgc caucauccgg caguacgggu cccccgcgcg cguccacauc gagaccggcc     1620

gggagcuggg caagagcuuc aaggagcgcc gggagaucca gaagcagcag gaggacaacc     1680

gcacgaagcg ggaguccgcc gugcagaagu ucaaggagcu guucagcgac uucuccagcg     1740

agcccaaguc caaggacauc cugaaguucc gccuguacga gcagcagcac gggaagugcc     1800

ucuacagcgg gaaggagauc aacauccacc gccugaacga gaagggcuac guggagaucg     1860

accacgcccu gccguucucc cggaccuggg acgacagcuu caacaacaag guccucgugc     1920

uggccuccga gaaccagaac aagggcaacc agacccccua cgaguggcug caggggaaga     1980

ucaacagcga gcgcuggaag aacuucgugg cccucguccu gggcucccag ugcagcgcgg     2040

ccaagaagca gcggcugcuc acccagguga ucgacgacaa caaguucauc gaccgcaacc     2100

ugaacgacac gcgguacauc gcccgcuucc uguccaacua cauccaggag aaccuccugc     2160

uggucgggaa gaacaagaag aacguguuca cccccaacgg ccagaucacc gcccuccugc     2220

ggagccgcug ggggcugauc aaggcgcggg agaacaacaa ccgccaccac gcccucgacg     2280

ccaucguggu cgccugcgcc acccccucca ugcagcagaa gaucacgcgg uucauccgcu     2340

ucaaggaggu gcacccguac aagaucgaga accgguacga gaugguggac caggagagcg     2400

gcgagaucau cagcccccac uuccccgagc ccugggcgua cuuccgccag gaggucaaca     2460

uccggguguu cgacaaccac ccggacaccg ugcugaagga gaugcugccc gaccgccccc     2520

aggccaacca ccaguucguc cagccccucu ucgugucccg ggccccgacc cgcaagauga     2580

gcggccaggg gcacauggag accaucaagu ccgccaagcg gcuggccgag ggcaucagcg     2640

ugcugcgcau cccccucacg cagcugaagc ccaaccugcu cgagaacaug gucaacaagg     2700

agcgggagcc ggcgcuguac gccgggcuga aggcccgccu cgccgaguuc aaccaggacc     2760

ccgccaaggc guucgccacc cccuucuaca agcagggcgg ccagcagguc aaggccaucc     2820

gcguggagca gguccagaag uccggggugc uggugcggga gaacaacggc guggccgaca     2880

acgcgagcau cgugcgcacc gacguguuca ucaagaacaa caaguucuuc cuggucccca     2940

ucuacaccug gcaggucgcc aaggggaucc ucccgaacaa ggccaucgug gcccacaaga     3000

acgaggacga gugggaggag auggacgagg gcgccaaguu caaguucucc cuguucccca     3060

acgaccuggu ggagcucaag acgaagaagg aguacuucuu cggguacuac aucggccugg     3120

accgggcgac cggcaacauc agccugaagg agcacgacgg ggagaucucc aagggcaagg     3180

acggggugua ccgcgugggc gucaagcucg cccugagcuu cgagaaguac cagguggacg     3240

agcuggggaa gaaccggcag aucugccgcc cccagcagcg gcagcccgug cgcaagcggc     3300

cggccgccac caagaaggcc ggccaggcga agaagaagaa guaaggacua gugaagggaa     3360

cacccaaauu uaauucagcc uuaagcacaa uuaauuaaga gugaaacgua auuguacaag     3420

caguugguca cccaccauag ggcaugauca acaccgcaac cuuuccuuuu ucccccagug     3480

auucugaaaa accccucuuc ccuucagcuu gcuuagaugu uccaaauuua guaagcuuaa     3540

ggcggccuac agaagaaaaa gaaaaaaaag gccacaaaag uucccucuca cuuucaguaa     3600

auaaaauaaa agcagcaaca gaaauaaaga aauaaaugaa auucaaaaug aaauaaauau     3660

uguguugugc agcauuaaaa aaucaauaaa aauuaaaaau gagcaagauc uaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3780


<210>  2340
<211>  3858
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2340
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc cgccuucaag cccaacccca ucaacuacau ccugggccuc gacaucggga      180

ucgccagcgu gggcugggcc augguggaga ucgacgagga cgagaacccc aucugccuga      240

ucgaccuggg gguccgggug uucgagcgcg cggaggugcc gaagaccggc gacucccucg      300

ccauggcccg gcgccuggcc cggagcgucc gccggcugac ccgccggcgc gcgcaccggc      360

uccugcgcgc ccggcgccug cucaagcggg agggcgugcu gcaggccgcc gacuucgacg      420

agaacgggcu gaucaagucc cuccccaaca cgcccuggca gcugcgcgcc gcggcccugg      480

accggaagcu cacgccccug gaguggagcg ccgugcugcu ccaccugauc aagcaccgcg      540

gcuaccuguc ccagcggaag aacgaggggg agaccgccga caaggagcuc ggcgcccugc      600

ugaagggggu cgcggacaac gcccacgccc uccagaccgg cgacuuccgc accccggccg      660

agcuggcccu gaacaaguuc gagaaggaga gcggccacau ccggaaccag cgcggggacu      720

acucccacac cuucagccgc aaggaccucc aggcggagcu gauccuccuc uucgagaagc      780

agaaggaguu cggcaacccc cacguguccg ggggccugaa ggagggcauc gagacccugc      840

ucaugaccca gcggcccgcc cugagcgggg acgccgugca gaagaugcug ggccacugca      900

cguucgagcc cgccgagccg aaggccgcga agaacaccua caccgccgag cgcuucaucu      960

ggcucaccaa gcugaacaac cugcggaucc ucgagcaggg guccgagcgc ccccugacgg     1020

acaccgagcg ggccacccug auggacgagc ccuaccgcaa gagcaagcuc accuacgccc     1080

aggcgcggaa gcugcugggc cucgaggaca cggccuucuu caaggggcug cgcuacggca     1140

aggacaacgc cgaggccucc acccugaugg agaugaaggc cuaccacgcg aucagccggg     1200

cccucgagaa ggagggccug aaggacaaga aguccccgcu gaaccugagc cccgagcucc     1260

aggacgagau cgggaccgcc uucucccugu ucaagaccga cgaggacauc acgggccgcc     1320

ugaaggaccg gauccagccc gagauccucg aggcccugcu gaagcacauc agcuucgaca     1380

aguucgucca gaucucccuc aaggcccugc gccggaucgu gccccugaug gagcagggga     1440

agcgcuacga cgaggcgugc gccgagaucu acggcgacca cuacgggaag aagaacaccg     1500

aggagaagau cuaccugccc ccgauccccg ccgacgagau ccggaacccc gugguccugc     1560

gcgcccugag ccaggcccgg aaggugauca acggcguggu ccgccgguac ggcagccccg     1620

cgcgcaucca caucgagacc gcccgcgagg uggggaaguc cuucaaggac cggaaggaga     1680

ucgagaagcg ccaggaggag aaccggaagg accgcgagaa ggccgccgcg aaguuccggg     1740

aguacuuccc gaacuucgug ggcgagccca agagcaagga cauccucaag cugcgccugu     1800

acgagcagca gcacgggaag ugccucuacu ccggcaagga gaucaaccug gggcggcuga     1860

acgagaaggg cuacgucgag aucgaccacg cccuccccuu cagccgcacc ugggacgacu     1920

ccuucaacaa caaggugcug gugcugggca gcgagaacca gaacaagggg aaccagacgc     1980

ccuacgagua cuucaacggc aaggacaacu cccgggagug gcaggaguuc aaggcccgcg     2040

ucgagaccag ccgguucccg cgcuccaaga agcagcggau ccuccugcag aaguucgacg     2100

aggacggguu caaggagcgc aaccugaacg acacccggua cgugaaccgc uuccucugcc     2160

aguucgucgc cgaccggaug cgccugaccg gcaagggcaa gaagcgggug uucgccagca     2220

acgggcagau cacgaaccug cuccgcggcu ucugggggcu gcggaaggug cgcgcggaga     2280

acgaccgcca ccacgcccug gacgccgucg ugguggccug cuccaccguc gccaugcagc     2340

agaagaucac ccgguucgug cgcuacaagg agaugaacgc guucgacggc aagaccaucg     2400

acaaggagac gggggaggug cuccaccaga agacccacuu cccccagccc ugggaguucu     2460

ucgcccagga ggucaugauc cggguguucg gcaagccgga cggcaagccc gaguucgagg     2520

aggccgacac ccccgagaag cugcgcaccc ugcucgccga gaagcugagc ucccggcccg     2580

aggccgugca cgaguacguc acgccgcugu ucgugagccg cgcgcccaac cggaagaugu     2640

ccgggcaggg ccacauggag accgucaaga gcgccaagcg ccucgacgag gggguguccg     2700

ugcugcgggu gccccugacc cagcucaagc ugaaggaccu ggagaagaug gucaaccgcg     2760

agcgggagcc caagcucuac gaggcccuga aggcccgccu ggaggcgcac aaggacgacc     2820

cggccaaggc cuucgccgag cccuucuaca aguacgacaa ggccggcaac cggacccagc     2880

aggucaaggc ggugcgcgug gagcaggucc agaagaccgg ggugugggug cggaaccaca     2940

acggcaucgc cgacaacgcc acgauggugc gcguggacgu cuucgagaag ggcgacaagu     3000

acuaccucgu gcccaucuac agcuggcagg ucgccaaggg gauccugccc gaccgggccg     3060

uggugcaggg caaggacgag gaggacuggc agcugaucga cgacuccuuc aacuucaagu     3120

ucagccucca cccgaacgac cugguggagg ucaucaccaa gaaggcgcgc auguucgggu     3180

acuucgccag cugccaccgg ggcaccggga acaucaacau ccgcauccac gaccuggacc     3240

acaagaucgg caagaacggc auccucgagg ggaucggcgu gaagaccgcc cuguccuucc     3300

agaaguacca gaucgacgag cuggggaagg agauccgccc cugccggcuc aagaagcgcc     3360

cgcccgugcg gaagcgcccc gccgccacga agaaggcggg ccaggccaag aagaagaagu     3420

aaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu aauuaagagu     3480

gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac accgcaaccu     3540

uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc uuagauguuc     3600

caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu     3660

cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau     3720

ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga     3780

gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaagaauu                                                   3858


<210>  2341
<211>  3864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2341
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa guaccacgug ggcaucgacg uggggaccuu cagcgucggc cuggccgcca      180

ucgaggugga cgacgccggg augcccauca agacccuguc ccuggugagc cacauccacg      240

acuccggccu ggaccccgac gagaucaaga gcgccgucac gcggcucgcg uccagcggca      300

ucgcccgccg gacccgccgg cuguaccgcc ggaagcgccg gcgccugcag cagcucgaca      360

aguucaucca gcggcagggg uggcccguga ucgagcugga ggacuacucc gacccgcugu      420

accccuggaa ggugcgcgcc gagcucgccg cgagcuacau cgccgacgag aaggagcggg      480

gcgagaagcu guccgucgcc cugcgccaca ucgcccggca ccgcgggugg cggaaccccu      540

acgccaaggu gagcucccuc uaccugcccg acggcccgag cgacgcguuc aaggccaucc      600

gcgaggagau caagcgggcc uccgggcagc ccgugcccga gaccgccacc gucggccaga      660

uggugacgcu gugcgagcuc ggcacccuga agcugcgcgg ggagggcggg gugcucagcg      720

cccggcugca gcaguccgac uacgcgcgcg agauccagga gaucugccgc augcaggaga      780

ucggccagga gcuguaccgg aagaucaucg acgucguguu cgccgccgag agccccaagg      840

gcuccgccag cucccgcgug gggaaggacc cgcuccagcc cggcaagaac cgggcccuga      900

aggcgagcga cgccuuccag cgcuaccgga ucgccgcccu gaucgggaac cuccgcgucc      960

ggguggacgg cgagaagcgc auccugagcg uggaggagaa gaaccugguc uucgaccacc     1020

ucgugaaccu gacccccaag aaggagcccg agugggugac caucgccgag auccugggga     1080

ucgaccgggg ccagcucauc ggcacggcga ccaugaccga cgacggggag cgcgccggcg     1140

cccggccgcc cacccacgac acgaaccgcu ccaucgucaa cagccggauc gcgccccugg     1200

uggacuggug gaagaccgcc uccgcccugg agcagcacgc cauggucaag gcccucagca     1260

acgccgaggu ggacgacuuc gacucccccg agggggcgaa ggugcaggcc uucuucgccg     1320

accuggacga cgacguccac gccaagcugg acagccucca ccucccggug ggccgcgccg     1380

cguacuccga ggacacccug gugcggcuca cccgccggau gcugagcgac ggggucgacc     1440

uguacacggc ccgccuccag gaguucggca ucgagcccuc cuggaccccg cccacgcccc     1500

ggaucggcga gcccgugggg aaccccgccg uggaccgcgu ccugaagacc gugagccgcu     1560

ggcuggaguc cgccaccaag accuggggcg cccccgagcg ggugaucauc gagcacgucc     1620

gcgagggguu cgugaccgag aagcgggcgc gcgagaugga cggcgacaug cggcgccggg     1680

ccgcccgcaa cgccaagcug uuccaggaga ugcaggagaa gcugaacgug caggggaagc     1740

cgagccgggc ggaccugugg cgcuaccagu ccguccagcg gcagaacugc cagugcgccu     1800

acugcggcag ccccaucacc uucuccaaca gcgagaugga ccacaucgug ccccgcgccg     1860

gccagggguc cacgaacacc cgggagaacc ucguggccgu cugccaccgc ugcaaccaga     1920

gcaagggcaa caccccguuc gccaucuggg cgaagaacac cuccaucgag ggggugagcg     1980

ugaaggaggc cgucgagcgg acgcgccacu gggugaccga caccggcaug cggagcaccg     2040

acuucaagaa guucacgaag gccguggucg agcgcuucca gcgggccacc auggacgagg     2100

agaucgacgc ccgcuccaug gagagcgugg cguggauggc caacgagcug cggucccgcg     2160

uggcccagca cuucgccagc cacggcacca ccguccgcgu guaccggggg ucccugacgg     2220

ccgaggcgcg ccgggccagc ggcaucuccg ggaagcugaa guucuucgac ggcgugggca     2280

agagccgccu cgaccggcgc caccacgcca ucgacgccgc ggucaucgcc uucaccuccg     2340

acuacguggc cgagacccug gccgugcgga gcaaccugaa gcagucccag gcccaccgcc     2400

aggaggcgcc ccaguggcgg gaguucaccg ggaaggacgc cgagcaccgc gccgccuggc     2460

gggucuggug ccagaagaug gagaagcuca gcgcccugcu gaccgaggac cuccgcgacg     2520

accggguggu ggucaugucc aacgugcgcc ugcggcuggg caacgggagc gcgcacaagg     2580

agacgaucgg caagcucucc aaggugaagc ugagcuccca gcugagcguc agcgacaucg     2640

acaaggccuc cagcgaggcc cucuggugcg cccugacccg cgagcccggg uucgacccca     2700

aggagggccu gccggccaac cccgagcggc acauccgcgu gaacgggacc cacguguacg     2760

cgggcgacaa caucggccuc uuccccgucu ccgccgggag caucgcccug cggggcgggu     2820

acgccgagcu gggcuccagc uuccaccacg cccgcgugua caagaucacc uccgggaaga     2880

agcccgcguu cgccaugcuc cgcguguaca cgaucgaccu gcugccguac cggaaccagg     2940

accucuucag cgucgagcug aagccccaga ccauguccau gcgccaggcc gagaagaagc     3000

ugcgggacgc ccucgcgacc ggcaacgccg aguaccuggg cuggcuggug guggacgacg     3060

agcucgucgu ggacaccagc aagaucgcca cggaccaggu gaaggccguc gaggccgagc     3120

uggggaccau ccgccggugg cgcguggacg gcuucuucuc ccccagcaag cugcggcucc     3180

gcccccugca gauguccaag gaggggauca agaaggagag cgcgccggag cuguccaaga     3240

ucaucgaccg gcccggcugg cuccccgccg ucaacaagcu guucagcgac gggaacguga     3300

ccgugguccg ccgggacucc cugggccgcg ugcggcucga gagcaccgcc caccuccccg     3360

ugacguggaa gguccagaag cgcccggccg ccaccaagaa ggcgggccag gccaagaaga     3420

agaaguaagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc acaauuaauu     3480

aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug aucaacaccg     3540

caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca gcuugcuuag     3600

auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa aaaggccaca     3660

aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua aagaaauaaa     3720

ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa uaaaaauuaa     3780

aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaag aauu                                            3864


<210>  2342
<211>  3564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2342
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc ccggauccug gccuucgaca ucggcaucag cuccaucggg ugggccuuca      180

gcgagaacga cgagcucaag gacugcggcg ugcgcaucuu caccaaggug gagaacccca      240

agaccgggga gucccuggcc cugccccggc gccucgcgcg gagcgcccgc aagcggcugg      300

cccgccggaa ggcccgccug aaccaccuca agcaccugau cgcgaacgag uucaagcuga      360

acuacgagga cuaccagucc uucgacgaga gccucgccaa ggccuacaag ggcucccuga      420

ucagccccua cgagcugcgg uuccgcgccc ucaacgagcu gcuguccaag caggacuucg      480

cccgggucau ccuccacauc gcgaagcgcc ggggcuacga cgacaucaag aacagcgacg      540

acaaggagaa gggggccauc cugaaggcca ucaagcagaa cgaggagaag cuggccaacu      600

accaguccgu gggcgaguac cucuacaagg aguacuucca gaaguucaag gagaacagca      660

aggaguucac gaacgugcgc aacaagaagg aguccuacga gcggugcauc gcccagagcu      720

uccugaagga cgagcugaag cucaucuuca agaagcagcg cgaguucggg uucuccuuca      780

gcaagaaguu cgaggaggag guccuguccg uggcguucua caagcgggcc cugaaggacu      840

ucagccaccu cgugggcaac ugcuccuucu ucaccgacga gaagcgcgcc ccgaagaaca      900

gcccccuggc cuucauguuc gucgcccuga cccggaucau caaccuccug aacaaccuga      960

agaacaccga ggggauccuc uacacgaagg acgaccugaa cgcgcugcuc aacgaggugc     1020

ugaagaacgg cacccugacc uacaagcaga ccaagaagcu ccugggccug agcgacgacu     1080

acgaguucaa gggggagaag ggcacguacu ucaucgaguu caagaaguac aaggaguuca     1140

ucaaggcccu cggggagcac aaccuguccc aggacgaccu gaacgagauc gccaaggaca     1200

ucacccucau caaggacgag aucaagcuga agaaggcccu ggccaaguac gaccucaacc     1260

agaaccagau cgacagccug uccaagcugg aguucaagga ccaccucaac aucagcuuca     1320

aggcgcugaa gcuggugacg ccccugaugc ucgagggcaa gaaguacgac gaggccugca     1380

acgagcugaa ccugaagguc gccaucaacg aggacaagaa ggacuuccuc cccgccuuca     1440

acgagaccua cuacaaggac gaggugacca acccgguggu ccugcgcgcg aucaaggagu     1500

accgcaaggu gcugaacgcc cuccugaaga aguacggcaa ggugcacaag aucaacaucg     1560

agcuggcccg ggaggucggg aagaaccacu cccagcgcgc caagaucgag aaggagcaga     1620

acgagaacua caaggccaag aaggacgcgg agcucgagug cgagaagcug ggccugaaga     1680

ucaacagcaa gaacauccuc aagcugcggc uguucaagga gcagaaggag uucugcgccu     1740

acuccgggga gaagaucaag aucagcgacc uccaggacga gaagaugcug gagaucgacc     1800

acaucuaccc cuacucccgc agcuucgacg acuccuacau gaacaaggug cugguguuca     1860

ccaagcagaa ccaggagaag cucaaccaga cccccuucga ggccuucggc aacgacagcg     1920

ccaaguggca gaagaucgag guccuggcca agaaccugcc caccaagaag cagaagcgga     1980

uccucgacaa gaacuacaag gacaaggagc agaagaacuu caaggaccgc aaccugaacg     2040

acacgcggua caucgcgcgc cuggugcuca acuacaccaa ggacuaccug gacuuccugc     2100

cgcucuccga cgacgagaac accaagcuga acgacaccca gaaggggagc aaggugcacg     2160

ucgaggccaa guccggcaug cugacgagcg cccuccggca caccuggggc uucuccgcca     2220

aggaccgcaa caaccaccuc caccacgcca ucgacgcggu gaucaucgcc uacgccaaca     2280

acagcaucgu caaggccuuc uccgacuuca agaaggagca ggagagcaac agcgcggagc     2340

uguacgccaa gaagaucucc gagcuggacu acaagaacaa gcggaaguuc uucgagcccu     2400

ucagcggguu ccgccagaag gugcuggaca agaucgacga gaucuucgug uccaagcccg     2460

agcggaagaa gcccagcggc gcccugcacg aggagaccuu ccgcaaggag gaggaguucu     2520

accaguccua cgggggcaag gagggggucc ucaaggcccu ggagcugggc aagauccgga     2580

aggugaacgg caagaucgug aagaacgggg acauguuccg cgucgacauc uucaagcaca     2640

agaagaccaa caaguucuac gccgugccga ucuacacgau ggacuucgcg cucaaggugc     2700

ugcccaacaa ggccgucgcc cggagcaaga agggcgagau caaggacugg auccugaugg     2760

acgagaacua cgaguucugc uucucccucu acaaggacag ccugauccug auccagacca     2820

aggacaugca ggagcccgag uucguguacu acaacgccuu caccuccagc accguguccc     2880

ucaucgucag caagcacgac aacaaguucg agacgcuguc caagaaccag aagauccugu     2940

ucaagaacgc caacgagaag gaggugaucg ccaagagcau cgggauccag aaccucaagg     3000

uguucgagaa guacaucguc uccgcgcugg gcgaggugac caaggccgag uuccgccagc     3060

gggaggacuu caagaagaag cgccccgccg ccaccaagaa ggcggggcag gccaagaaga     3120

agaaguaagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc acaauuaauu     3180

aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug aucaacaccg     3240

caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca gcuugcuuag     3300

auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa aaaggccaca     3360

aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua aagaaauaaa     3420

ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa uaaaaauuaa     3480

aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaag aauu                                            3564


<210>  2343
<211>  3975
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2343
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgag cgaccuggug cucggccugg acaucgggau cggcuccgug ggggucggca      180

uccugaacaa ggugaccggc gagaucaucc acaagaacag ccggaucuuc cccgccgccc      240

aggccgagaa caaccucgug cgccggacca accgccaggg gcggcgccug gcccggcgca      300

agaagcaccg gcgcguccgg cugaaccgcc ucuucgagga guccggccug aucacggacu      360

ucaccaagau cagcaucaac cugaaccccu accagcuccg ggugaagggg cugaccgacg      420

agcuguccaa cgaggagcug uucaucgcgc ugaagaacau ggugaagcac cgcggcauca      480

gcuaccugga cgacgccucc gacgacggga acagcuccgu cggcgacuac gcccagaucg      540

ugaaggagaa cagcaagcag cucgagacca agacgcccgg ccagauccag cuggagcggu      600

accagaccua cgggcagcug cgcggcgacu ucaccgugga gaaggacggg aagaagcacc      660

ggcucaucaa cgucuucccg accuccgccu accgcagcga ggcgcugcgg auccugcaga      720

cgcagcagga guucaacccc cagaucaccg acgaguucau caaccgcuac cucgagaucc      780

ugaccggcaa gcggaaguac uaccacggcc ccgggaacga gaagucccgc accgacuacg      840

gccgcuaccg gacgagcggg gagacccugg acaacaucuu cggcauccuc aucgggaagu      900

gcaccuucua ccccgacgag uuccgcgccg ccaaggccuc cuacaccgcc caggaguuca      960

accugcugaa cgaccucaac aaccugacgg ugccgaccga gaccaagaag cugagcaagg     1020

agcagaagaa ccagaucauc aacuacguga agaacgagaa ggcgaugggc cccgccaagc     1080

uguucaagua caucgccaag cuccuguccu gcgacgucgc cgacaucaag ggcuaccgga     1140

ucgacaagag cgggaaggcc gagauccaca ccuucgaggc guaccgcaag augaagacgc     1200

ucgagacccu ggacaucgag cagauggacc gggagacccu ggacaagcuc gccuacgugc     1260

ugacccugaa cacggagcgc gagggcaucc aggaggcccu cgagcacgag uucgccgacg     1320

gguccuucag ccagaagcag gucgacgagc uggugcaguu ccggaaggcc aacagcucca     1380

ucuucggcaa gggguggcac aacuucagcg ugaagcugau gauggagcuc auccccgagc     1440

uguacgagac cuccgaggag cagaugacca uccugacccg ccucggcaag cagaagacga     1500

ccagcuccag caacaagacc aaguacaucg acgagaagcu gcugaccgag gagaucuaca     1560

accccguggu cgcgaagucc gugcggcagg ccaucaagau cgugaacgcc gccaucaagg     1620

aguacggcga cuucgacaac aucgucaucg agauggcccg cgagacgaac gaggacgacg     1680

agaagaaggc gauccagaag auccagaagg ccaacaagga cgagaaggac gccgccaugc     1740

ucaaggcggc caaccaguac aacgggaagg ccgagcugcc gcacagcgug uuccacggcc     1800

acaagcagcu ggccaccaag auccggcugu ggcaccagca gggggagcgc ugccucuaca     1860

ccggcaagac caucuccauc cacgaccuga ucaacaacag caaccaguuc gaggucgacc     1920

acauccugcc ccucuccauc acguucgacg acagccuggc caacaaggug cugguguacg     1980

cgaccgccaa ccaggagaag gggcagcgga cccccuacca ggcccucgac uccauggacg     2040

acgccuggag cuuccgcgag cugaaggccu ucguccggga guccaagacc cugagcaaca     2100

agaagaagga guaccuccug accgaggagg acaucuccaa guucgacgug cgcaagaagu     2160

ucaucgagcg gaaccuggug gacacgcgcu acgcgagccg ggucgugcuc aacgcccugc     2220

aggagcacuu ccgcgcccac aagaucgaca ccaagguguc cgucgugcgc ggccaguuca     2280

ccagccagcu gcggcgccac uggggcaucg agaagacccg ggacacguac caccaccacg     2340

ccguggacgc ccucaucauc gcggccagcu cccagcugaa ccuguggaag aagcagaaga     2400

acacccucgu cagcuacucc gaggaccagc ugcuggacau cgagaccggg gagcucauca     2460

gcgacgacga guacaaggag uccguguuca aggcccccua ccagcacuuc guggacaccc     2520

ucaagagcaa ggaguucgag gacuccaucc uguucagcua ccaggucgac uccaaguuca     2580

accgcaagau cagcgacgcc acgaucuacg cgacccggca ggccaaggug ggcaaggaca     2640

aggccgacga gaccuacgug cucgggaaga ucaaggacau cuacacccag gacggcuacg     2700

acgccuucau gaagaucuac aagaaggaca aguccaaguu ccugauguac cgccacgacc     2760

cgcagacguu cgagaagguc aucgagccca uccuggagaa cuaccccaac aagcagauca     2820

acgagaaggg caaggaggug cccugcaacc cguuccucaa guacaaggag gagcacgggu     2880

acauccggaa guacagcaag aagggcaacg ggcccgagau caagucccug aaguacuacg     2940

acagcaagcu gggcaaccac aucgacauca cccccaagga cuccaacaac aagguggucc     3000

uccagagcgu gucccccugg cgcgccgacg uguacuucaa caagaccacc gggaaguacg     3060

agauccuggg ccugaaguac gcggaccucc aguucgagaa gggcacgggg accuacaaga     3120

ucagccagga gaaguacaac gacaucaaga agaaggaggg cgucgacagc gacuccgagu     3180

ucaaguucac ccuguacaag aacgaccugc uccuggugaa ggacaccgag acgaaggagc     3240

agcagcuguu ccgguuccuc agccgcacca ugccgaagca gaagcacuac guggagcuga     3300

agcccuacga caagcagaag uucgagggcg gggaggcccu gaucaagguc cucgggaacg     3360

uggccaacuc cggccagugc aagaagggcc ugggcaagag caacaucucc aucuacaagg     3420

ugcggaccga cguccugggg aaccagcaca ucaucaagaa cgagggggac aagcccaagc     3480

ucgacuucaa gcgccccgcc gccaccaaga aggcgggcca ggccaagaag aagaaguaag     3540

gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau uaagagugaa     3600

acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc gcaaccuuuc     3660

cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa     3720

auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc     3780

ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa augaaauuca     3840

aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua aaaaugagca     3900

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3960

aaaaaaaaaa gaauu                                                      3975


<210>  2344
<211>  4533
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2344
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgac ccaguucgag ggcuucacca accuguacca ggugagcaag acgcuccggu      180

ucgagcugau cccccagggg aagacccuga agcacaucca ggagcagggc uucaucgagg      240

aggacaaggc ccgcaacgac cacuacaagg agcucaagcc caucaucgac cggaucuaca      300

agaccuacgc cgaccagugc cugcagcugg ugcagcucga cugggagaac cuguccgccg      360

ccaucgacag cuaccgcaag gagaagaccg aggagacgcg gaacgcgcug aucgaggagc      420

aggccaccua ccgcaacgcc auccacgacu acuucaucgg gcggaccgac aaccucaccg      480

acgccaucaa caagcgccac gcggagaucu acaagggccu guucaaggcc gagcuguuca      540

acggcaaggu ccucaagcag cuggggacgg ugaccaccac cgagcacgag aacgcccugc      600

uccgguccuu cgacaaguuc acgaccuacu ucagcggcuu cuacgagaac cgcaagaacg      660

uguucuccgc cgaggacauc agcaccgcca ucccccaccg gaucguccag gacaacuucc      720

cgaaguucaa ggagaacugc cacaucuuca cccgccugau cacggcggug cccucccugc      780

gggagcacuu cgagaacgug aagaaggcca ucgggaucuu cgucagcacc uccaucgagg      840

agguguucag cuuccccuuc uacaaccagc uccugaccca gacccagauc gaccuguaca      900

accagcuccu gggcgggauc ucccgcgagg ccggcacgga gaagaucaag ggccugaacg      960

aggugcucaa ccuggccauc cagaagaacg acgagaccgc ccacaucauc gcgagccugc     1020

cccaccgguu caucccccuc uucaagcaga uccuguccga ccgcaacacc cugagcuuca     1080

uccucgagga guucaagucc gacgaggagg ucauccagag cuucugcaag uacaagaccc     1140

ugcugcggaa cgagaacgug cucgagacgg ccgaggcccu guucaacgag cugaacucca     1200

ucgaccucac ccacaucuuc aucagccaca agaagcugga gaccaucucc agcgcccugu     1260

gcgaccacug ggacacccuc cgcaacgccc uguacgagcg gcgcaucagc gagcugacgg     1320

ggaagaucac caaguccgcg aaggagaagg ugcagcggag ccucaagcac gaggacauca     1380

accugcagga gaucaucucc gccgccggca aggagcugag cgaggccuuc aagcagaaga     1440

ccuccgagau ccucagccac gcccacgcgg cccuggacca gccgcugccc accacgcuca     1500

agaagcagga ggagaaggag auccugaagu cccagcugga cagccugcuc gggcuguacc     1560

accuccucga cugguucgcc gucgacgagu ccaacgaggu ggaccccgag uucagcgccc     1620

gccugaccgg caucaagcug gagauggagc cgucccucag cuucuacaac aaggcgcgca     1680

acuacgccac caagaagccc uacuccgugg agaaguucaa gcugaacuuc cagaugccca     1740

cccuggccag cggcugggac gucaacaagg agaagaacaa cggggccauc cucuucguga     1800

agaacggccu guacuaccug gggaucaugc ccaagcagaa gggccgguac aaggcccucu     1860

ccuucgagcc gacggagaag accagcgagg gguucgacaa gauguacuac gacuacuucc     1920

ccgacgcggc caagaugauc cccaagugcu ccacccagcu gaaggccgug accgcccacu     1980

uccagaccca cacgaccccc auccugcuca gcaacaacuu caucgagccg cuggagauca     2040

ccaaggagau cuacgaccug aacaaccccg agaaggagcc caagaaguuc cagaccgccu     2100

acgcgaagaa gacgggcgac cagaagggcu accgcgaggc ccucugcaag uggaucgacu     2160

ucacccggga cuuccugucc aaguacacca agaccacgag caucgaccug agcucccucc     2220

gccccagcuc ccaguacaag gaccuggggg aguacuacgc cgagcugaac ccgcuccugu     2280

accacaucag cuuccagcgg aucgccgaga aggagaucau ggacgccguc gagaccggca     2340

agcuguaccu cuuccagauc uacaacaagg acuucgcgaa ggggcaccac ggcaagccca     2400

accugcacac ccuguacugg accggccucu ucucccccga gaaccuggcc aagacgagca     2460

ucaagcugaa cgggcaggcc gagcuguucu accgccccaa gucccggaug aagcgcaugg     2520

cccaccggcu gggcgagaag augcugaaca agaagcucaa ggaccagaag accccgaucc     2580

ccgacacccu guaccaggag cuguacgacu acgugaacca ccgccucagc cacgaccugu     2640

ccgacgaggc gcgggcccug cuccccaacg ugaucaccaa ggaggucagc cacgagauca     2700

ucaaggaccg ccgguucacg uccgacaagu ucuucuucca cgugccgauc acccugaacu     2760

accaggccgc caacagcccc uccaaguuca accagcgcgu caacgccuac cugaaggagc     2820

accccgagac ccccaucauc gggaucgacc ggggcgagcg caaccucauc uacaucaccg     2880

ugaucgacag cacggggaag auccuggagc agcggucccu gaacaccauc cagcaguucg     2940

acuaccagaa gaagcucgac aaccgcgaga aggagcgggu ggcggcccgc caggccugga     3000

gcgucguggg caccaucaag gaccugaagc agggguaccu gucccaggug auccacgaga     3060

ucgucgaccu caugauccac uaccaggccg uggugguccu ggagaaccug aacuucggcu     3120

ucaagagcaa gcgcaccggc aucgccgaga aggcggugua ccagcaguuc gagaagaugc     3180

ucaucgacaa gcugaacugc cuggugcuca aggacuaccc ggccgagaag gucgggggcg     3240

ugcugaaccc cuaccagcug acggaccagu ucaccagcuu cgccaagaug gggacccagu     3300

ccggcuuccu cuucuacgug cccgcccccu acaccagcaa gaucgacccg cugacgggcu     3360

ucgucgaccc cuucgugugg aagaccauca agaaccacga gucccggaag cacuuccugg     3420

agggguucga cuuccuccac uacgacguga agaccggcga cuucauccug cacuucaaga     3480

ugaaccgcaa ccugagcuuc cagcgggggc uccccggcuu caugcccgcc ugggacaucg     3540

ucuucgagaa gaacgagacc caguucgacg cgaaggggac gccguucauc gccggcaagc     3600

gcaucgugcc cgugaucgag aaccaccggu ucaccggccg cuaccgggac cuguaccccg     3660

ccaacgagcu gaucgcccuc cuggaggaga aggggaucgu cuuccgcgac ggcuccaaca     3720

uccugcccaa gcuccuggag aacgacgaca gccacgcgau cgacaccaug guggcccuga     3780

uccgguccgu gcuccagaug cgcaacagca acgccgccac cggggaggac uacaucaacu     3840

ccccgguccg ggaccugaac ggcgugugcu ucgacagccg cuuccagaac cccgaguggc     3900

ccauggacgc cgacgcgaac ggggccuacc acaucgcccu gaagggccag cuccugcuga     3960

accaccucaa ggaguccaag gaccugaagc ugcagaacgg caucagcaac caggacuggc     4020

ucgccuacau ccaggagcug cggaacaagc gccccgccgc gacgaagaag gccgggcagg     4080

ccaagaagaa gaaguaagga cuagugaagg gaacacccaa auuuaauuca gccuuaagca     4140

caauuaauua agagugaaac guaauuguac aagcaguugg ucacccacca uagggcauga     4200

ucaacaccgc aaccuuuccu uuuuccccca gugauucuga aaaaccccuc uucccuucag     4260

cuugcuuaga uguuccaaau uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa     4320

aaggccacaa aaguucccuc ucacuuucag uaaauaaaau aaaagcagca acagaaauaa     4380

agaaauaaau gaaauucaaa augaaauaaa uauuguguug ugcagcauua aaaaaucaau     4440

aaaaauuaaa aaugagcaag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  4533


<210>  2345
<211>  4512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2345
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgag caucuaccag gaguucguga acaaguacuc ccugagcaag acccuccggu      180

ucgagcugau cccccagggc aagacccugg agaacaucaa ggcccgcggg cucauccugg      240

acgacgagaa gcgggccaag gacuacaaga aggccaagca gaucaucgac aaguaccacc      300

aguucuucau cgaggagauc cuguccagcg ugugcaucuc cgaggaccuc cugcagaacu      360

acagcgacgu cuacuucaag cugaagaagu ccgacgacga caaccuccag aaggacuuca      420

agagcgccaa ggacacgauc aagaagcaga ucuccgagua caucaaggac agcgagaagu      480

ucaagaaccu guucaaccag aaccugaucg acgcgaagaa gggccaggag uccgaccuca      540

uccuguggcu gaagcagagc aaggacaacg ggaucgagcu guucaaggcc aacuccgaca      600

ucaccgacau cgacgaggcc cucgagauca ucaagagcuu caagggcugg accaccuacu      660

ucaagggcuu ccacgagaac cgcaagaacg uguacuccag caacgacauc cccacgucca      720

ucaucuaccg gaucguggac gacaaccugc ccaaguuccu cgagaacaag gccaaguacg      780

agagccugaa ggacaaggcg ccggaggcca ucaacuacga gcagaucaag aaggaccugg      840

ccgaggagcu caccuucgac aucgacuaca agaccuccga ggucaaccag cgcguguuca      900

gccuggacga gguguucgag aucgccaacu ucaacaacua ccugaaccag agcgggauca      960

ccaaguucaa cacgaucauc ggcgggaagu ucgucaacgg cgagaacacc aagcggaagg     1020

ggaucaacga guacaucaac cucuacuccc agcagaucaa cgacaagacc cugaagaagu     1080

acaagaugag cgugcuguuc aagcagaucc ucuccgacac cgagagcaag uccuucguga     1140

ucgacaagcu ggaggacgac agcgacgucg ugacgaccau gcaguccuuc uacgagcaga     1200

ucgccgcguu caagaccgug gaggagaagu ccaucaagga gacccugucc cuccuguucg     1260

acgaccugaa ggcccagaag cucgaccuga gcaagaucua cuucaagaac gacaaguccc     1320

ugacggaccu cagccagcag gucuucgacg acuacuccgu gaucggcacc gccgugcugg     1380

aguacaucac ccagcagauc gcccccaaga accuggacaa ccccagcaag aaggagcagg     1440

agcucaucgc caagaagacc gagaaggcga aguaccuguc ccuggagacg aucaagcucg     1500

cccuggagga guucaacaag caccgcgaca ucgacaagca gugccgguuc gaggagaucc     1560

uggccaacuu cgccgccauc cccaugaucu ucgacgagau cgcgcagaac aaggacaacc     1620

ucgcccagau cagcaucaag uaccagaacc agggcaagaa ggaccugcug caggccuccg     1680

ccgaggacga cgucaaggcc aucaaggacc uccuggacca gaccaacaac cugcugcaca     1740

agcucaagau cuuccacauc agccaguccg aggacaaggc gaacauccug gacaaggacg     1800

agcacuucua ccugguguuc gaggagugcu acuucgagcu cgccaacauc gugccgcugu     1860

acaacaagau ccgcaacuac aucacccaga agcccuacag cgacgagaag uucaagcuga     1920

acuucgagaa cagcacccuc gccaacgggu gggacaagaa caaggagccc gacaacacgg     1980

ccauccuguu caucaaggac gacaaguacu accugggcgu caugaacaag aagaacaaca     2040

agaucuucga cgacaaggcg aucaaggaga acaaggggga gggcuacaag aagaucgugu     2100

acaagcuccu gcccggcgcc aacaagaugc ugccgaaggu guucuucucc gccaagagca     2160

ucaaguucua caaccccucc gaggacaucc uccggauccg caaccacagc acccacacca     2220

agaacgggag cccccagaag ggcuacgaga aguucgaguu caacaucgag gacugccgga     2280

aguucaucga cuucuacaag cagagcaucu ccaagcaccc cgaguggaag gacuucgggu     2340

uccgcuucag cgacacccag cgguacaacu ccaucgacga guucuaccgc gaggucgaga     2400

accagggcua caagcugacg uucgagaaca ucagcgaguc cuacaucgac agcgugguca     2460

accaggggaa gcuguaccuc uuccagaucu acaacaagga cuucuccgcc uacagcaagg     2520

gccggccgaa ccugcacacc cuguacugga aggcccucuu cgacgagcgc aaccugcagg     2580

acguggugua caagcugaac ggcgaggcgg agcuguucua ccggaagcag uccaucccca     2640

agaagaucac ccaccccgcc aaggaggcca ucgccaacaa gaacaaggac aaccccaaga     2700

aggagagcgu cuucgaguac gaccucauca aggacaagcg cuucaccgag gacaaguucu     2760

ucuuccacug cccgaucacg aucaacuuca aguccagcgg ggccaacaag uucaacgacg     2820

agaucaaccu gcuccugaag gagaaggcga acgacgugca cauccugagc aucgaccggg     2880

gcgagcgcca ccucgccuac uacacccugg uggacgggaa gggcaacauc aucaagcagg     2940

acaccuucaa caucaucggg aacgaccgca ugaagaccaa cuaccacgac aagcuggccg     3000

ccaucgagaa ggaccgggac uccgcccgca aggacuggaa gaagaucaac aacaucaagg     3060

agaugaagga gggcuaccuc agccaggucg ugcacgagau cgcgaagcug gugaucgagu     3120

acaacgccau cgucguguuc gaggaccuga acuucggcuu caagcggggg cgcuucaagg     3180

uggagaagca ggucuaccag aagcucgaga agaugcugau cgagaagcug aacuaccucg     3240

uguucaagga caacgaguuc gacaagacgg gcggggugcu gcgggccuac cagcugaccg     3300

cccccuucga gaccuucaag aagaugggca agcagaccgg gaucaucuac uacguccccg     3360

cgggcuucac cuccaagauc ugccccguga cgggcuucgu gaaccagcuc uacccgaagu     3420

acgagagcgu cuccaagagc caggaguucu ucuccaaguu cgacaagauc ugcuacaacc     3480

uggacaaggg guacuucgag uucagcuucg acuacaagaa cuucggcgac aaggccgcca     3540

aggggaagug gaccaucgcc uccuucggca gccgccugau caacuuccgg aacuccgaca     3600

agaaccacaa cugggacacc cgcgaggugu accccaccaa ggagcucgag aagcugcuga     3660

aggacuacag caucgaguac ggccacgggg agugcaucaa ggccgcgauc ugcggcgagu     3720

ccgacaagaa guucuucgcc aagcucacga gcgugcugaa caccauccug cagaugcgga     3780

acuccaagac cgggaccgag cucgacuacc ugaucagccc cgucgccgac gugaacggca     3840

acuucuucga cucccgccag gcccccaaga acaugccgca ggacgccgac gcgaacgggg     3900

ccuaccacau cggccugaag ggccucaugc ugcuggggcg gaucaagaac aaccaggagg     3960

gcaagaagcu caaccuggug aucaagaacg aggaguacuu cgaguucguc cagaaccgca     4020

acaacaagcg gcccgccgcc acgaagaagg ccgggcaggc gaagaagaag aaguaaggac     4080

uagugaaggg aacacccaaa uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg     4140

uaauuguaca agcaguuggu cacccaccau agggcaugau caacaccgca accuuuccuu     4200

uuucccccag ugauucugaa aaaccccucu ucccuucagc uugcuuagau guuccaaauu     4260

uaguaagcuu aaggcggccu acagaagaaa aagaaaaaaa aggccacaaa aguucccucu     4320

cacuuucagu aaauaaaaua aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa     4380

ugaaauaaau auuguguugu gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga     4440

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaagaa uu                                                         4512


<210>  2346
<211>  4813
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2346
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggacaa gaaguacagc      240

aucggccugg acaucgggac caacuccgug ggcugggccg ugaucaccga cgaguacaag      300

guccccagca agaaguucaa ggugcugggg aacacggacc ggcacuccau caagaagaac      360

cugaucggcg cccugcuguu cgacagcggc gagaccgccg aggccacccg ccugaagcgg      420

accgcgcgcc ggcgcuacac gcggcgcaag aaccggaucu gcuaccugca ggagaucuuc      480

uccaacgaga uggccaaggu ggacgacagc uucuuccacc gccucgagga guccuuccug      540

gucgaggagg acaagaagca cgagcggcac cccaucuucg ggaacaucgu ggacgaggug      600

gccuaccacg agaaguaccc caccaucuac caccuccgca agaagcucgu cgacagcacc      660

gacaaggccg accugcggcu gaucuaccuc gcgcuggccc acaugaucaa guuccgcggc      720

cacuuccuga ucgaggggga ccucaacccg gacaacuccg acguggacaa gcuguucauc      780

cagcuggugc agaccuacaa ccagcuguuc gaggagaacc ccaucaacgc cagcggcguc      840

gacgccaagg ccauccuguc cgcgcggcug agcaaguccc gccggcucga gaaccugauc      900

gcccagcugc ccggggagaa gaagaacggc cucuucggca accugaucgc ccugagccuc      960

gggcugacgc ccaacuucaa guccaacuuc gaccuggccg aggacgccaa gcuccagcug     1020

agcaaggaca ccuacgacga cgaccuggac aaccuccugg cgcagaucgg cgaccaguac     1080

gccgaccugu uccucgccgc caagaaccug uccgacgcca uccugcucag cgacauccug     1140

cgcgugaaca ccgagaucac caaggcgccg cuguccgcca gcaugaucaa gcgguacgac     1200

gagcaccacc aggaccucac gcugcugaag gcccucgugc gccagcagcu gcccgagaag     1260

uacaaggaga ucuucuucga ccagagcaag aacggguacg ccggcuacau cgacggcggg     1320

gccucccagg aggaguucua caaguucauc aagcccaucc uggagaagau ggacggcacc     1380

gaggagcucc uggucaagcu gaaccgggag gaccugcucc gcaagcagcg caccuucgac     1440

aacgggagca ucccccacca gauccaccug ggcgagcugc acgcgauccu ccggcgccag     1500

gaggacuucu acccguuccu gaaggacaac cgggagaaga ucgagaagau ccugaccuuc     1560

cgcauccccu acuacguggg gccccucgcc cggggcaacu cccgcuucgc cuggaugacg     1620

cgcaagagcg aggagaccau cacccccugg aacuucgagg agguggucga caagggcgcc     1680

uccgcgcaga gcuucaucga gcggaugacc aacuucgaca agaaccugcc gaacgagaag     1740

gugcugccca agcacucccu ccuguacgag uacuucacgg uguacaacga gcugaccaag     1800

gucaaguacg ugaccgaggg gaugcggaag cccgccuucc ucagcggcga gcagaagaag     1860

gccaucgugg accugcuguu caagaccaac cgcaagguca cggugaagca gcucaaggag     1920

gacuacuuca agaagaucga gugcuucgac uccgucgaga ucagcggggu ggaggaccgg     1980

uucaacgccu cccugggcac cuaccacgac cugcucaaga ucaucaagga caaggacuuc     2040

cuggacaacg aggagaacga ggacauccug gaggacaucg ugcucacccu gacccuguuc     2100

gaggaccgcg agaugaucga ggagcggcuc aagacguacg cccaccuguu cgacgacaag     2160

gucaugaagc agcugaagcg ccggcgcuac accggguggg gccggcucag ccgcaagcug     2220

aucaacggca uccgggacaa gcaguccggg aagaccaucc uggacuuccu caagagcgac     2280

ggcuucgcga accgcaacuu caugcagcug auccacgacg acucccugac cuucaaggag     2340

gacauccaga aggcccaggu gagcgggcag ggcgacuccc uccacgagca caucgccaac     2400

cuggccggga gccccgccau caagaagggc auccugcaga cggugaaggu cguggacgag     2460

cucgugaagg ucaugggccg ccacaagccg gagaacaucg ugaucgagau ggcgcgggag     2520

aaccagacca cccagaaggg gcagaagaac ucccgcgagc ggaugaagcg caucgaggag     2580

ggcaucaagg agcuggggag ccagauccug aaggagcacc ccguggagaa cacccagcuc     2640

cagaacgaga agcuguaccu guacuaccuc cagaacggcc gggacaugua cgucgaccag     2700

gagcuggaca ucaaccgccu gagcgacuac gacguggacc acaucgugcc ccaguccuuc     2760

cucaaggacg acagcaucga caacaagguc cugacgcggu ccgacaagaa ccgcggcaag     2820

agcgacaacg ugcccuccga ggaggugguc aagaagauga agaacuacug gcggcagcug     2880

cucaacgcca agcugaucac ccagcgcaag uucgacaacc ugaccaaggc cgagcgcggg     2940

ggccucagcg agcuggacaa ggccggguuc aucaagcggc agcuggugga gacccggcag     3000

aucaccaagc acguggccca gauccucgac ucccgcauga acacgaagua cgacgagaac     3060

gacaagcuga uccgggaggu caaggugauc acccucaaga gcaagcucgu guccgacuuc     3120

cgcaaggacu uccaguucua caagguccgg gagaucaaca acuaccacca cgcgcacgac     3180

gccuaccuga acgccguggu gggcaccgcc cugaucaaga aguacccgaa gcucgagagc     3240

gaguucgucu acggggacua caagguguac gacgugcgca agaugaucgc gaaguccgag     3300

caggagaucg gcaaggccac cgccaaguac uucuucuaca gcaacaucau gaacuucuuc     3360

aagacggaga ucacccuggc caacggcgag auccggaagc gcccccugau cgagaccaac     3420

ggggagaccg gcgagaucgu cugggacaag gggcgggacu ucgccacggu gcgcaaggug     3480

cucuccaugc cccaggucaa caucgugaag aagaccgagg ugcagaccgg cggguucagc     3540

aaggagucca uccugcccaa gcgcaacagc gacaagcuga ucgcgcggaa gaaggacugg     3600

gacccgaaga aguacggcgg cuucgacucc cccaccgucg ccuacagcgu gcucgugguc     3660

gccaaggugg agaagggcaa gagcaagaag cugaaguccg ugaaggagcu gcucgggauc     3720

acgaucaugg agcgcagcuc cuucgagaag aaccccaucg acuuccugga ggccaagggg     3780

uacaaggagg ucaagaagga ccugaucauc aagcucccga aguacagccu guucgagcug     3840

gagaacggcc ggaagcgcau gcucgccucc gcgggggagc ugcagaaggg caacgagcug     3900

gcccucccca gcaaguacgu gaacuuccug uaccuggccu cccacuacga gaagcucaag     3960

ggcagccccg aggacaacga gcagaagcag cuguucgugg agcagcacaa gcacuaccug     4020

gacgagauca ucgagcagau cuccgaguuc agcaagcggg ucauccucgc cgacgccaac     4080

cuggacaagg ugcuguccgc guacaacaag caccgcgaca agcccauccg ggagcaggcc     4140

gagaacauca uccaccucuu cacccugacc aaccuggggg ccccggccgc guucaaguac     4200

uucgacacca cgaucgaccg caagcgguac accagcacca aggaggugcu cgacgccacc     4260

cugauccacc aguccaucac gggccuguac gagacccgca ucgaccucag ccagcucggg     4320

ggcgacaagc ggcccgccgc caccaagaag gccgggcagg cgaagaagaa gaaguaagga     4380

cuagugaagg gaacacccaa auuuaauuca gccuuaagca caauuaauua agagugaaac     4440

guaauuguac aagcaguugg ucacccacca uagggcauga ucaacaccgc aaccuuuccu     4500

uuuuccccca gugauucuga aaaaccccuc uucccuucag cuugcuuaga uguuccaaau     4560

uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa aaggccacaa aaguucccuc     4620

ucacuuucag uaaauaaaau aaaagcagca acagaaauaa agaaauaaau gaaauucaaa     4680

augaaauaaa uauuguguug ugcagcauua aaaaaucaau aaaaauuaaa aaugagcaag     4740

aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4800

aaaaaaaaga auu                                                        4813


<210>  2347
<211>  5596
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2347
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaacuu caagauccug      240

cccaucgcca ucgaccucgg cgugaagaac accggggugu ucagcgccuu cuaccagaag      300

ggcaccuccc uggagcggcu ggacaacaag aacgggaagg ucuacgagcu cagcaaggac      360

uccuacacgc ugcugaugaa caaccgcacc gcccggcgcc accagcggcg cggcaucgac      420

cggaagcagc ucgugaagcg ccuguucaag cugaucugga ccgagcagcu caaccuggag      480

ugggacaagg acacccagca ggccaucagc uuccuguuca accggcgcgg cuucuccuuc      540

aucacggacg gguacagccc cgaguaccuc aacaucgugc ccgagcaggu caaggcgauc      600

cugauggaca ucuucgacga cuacaacggc gaggacgacc uggacuccua ccucaagcug      660

gccaccgagc aggagagcaa gaucuccgag aucuacaaca agcugaugca gaagauccuc      720

gaguucaagc ugaugaagcu gugcaccgac aucaaggacg acaaggugag caccaagacg      780

cucaaggaga ucaccuccua cgaguucgag cugcuggccg acuaccucgc caacuacagc      840

gagucccuga agacccagaa guucagcuac accgacaagc aggggaaccu gaaggagcuc      900

uccuacuacc accacgacaa guacaacauc caggaguucc ugaagcggca cgcgacgauc      960

aacgaccgca uccuggacac ccuccugacc gacgaccugg acaucuggaa cuucaacuuc     1020

gagaaguucg acuucgacaa gaacgaggag aagcuccaga accaggagga caaggaccac     1080

auccaggccc accuccacca cuucguguuc gccgucaaca agaucaagag cgagauggcc     1140

uccggcgggc ggcaccgcag ccaguacuuc caggagauca ccaacgugcu ggacgagaac     1200

aaccaccagg agggcuaccu gaagaacuuc ugcgagaacc ugcacaacaa gaaguacucc     1260

aaccugagcg ugaagaaccu cgucaaccug aucggcaacc ugagcaaccu cgagcugaag     1320

ccgcugcgga aguacuucaa cgacaagauc cacgccaagg cggaccacug ggacgagcag     1380

aaguucacgg agaccuacug ccacuggauc cucggggagu ggcgcguggg cgugaaggac     1440

caggacaaga aggacggggc caaguacucc uacaaggacc ugugcaacga gcugaagcag     1500

aaggucacca aggccggccu cguggacuuc cugcuggagc uggaccccug ccggaccauc     1560

ccgcccuacc ucgacaacaa caaccgcaag ccgcccaagu gccagagccu gauccugaac     1620

cccaaguucc ucgacaacca guaccccaac uggcagcagu accugcagga gcugaagaag     1680

cuccagucca uccagaacua ccuggacagc uucgagacgg accugaaggu gcucaagucc     1740

agcaaggacc agcccuacuu cgucgaguac aaguccagca accagcagau cgccuccggc     1800

cagcgggacu acaaggaccu ggacgcccgc auccugcagu ucaucuucga ccgggugaag     1860

gcgagcgacg agcuccugcu gaacgagauc uacuuccagg ccaagaagcu caagcagaag     1920

gccuccagcg agcuggagaa gcuggagucc agcaagaagc ucgacgaggu gaucgccaac     1980

ucccagcuga gccagauccu gaagucccag cacaccaacg ggaucuucga gcagggcacc     2040

uuccuccacc uggucugcaa guacuacaag cagcgccagc gcgcccggga cagccgccug     2100

uacaucaugc ccgaguaccg guacgacaag aagcuccaca aguacaacaa caccgggcgc     2160

uucgacgacg acaaccagcu gcugacguac ugcaaccaca agccccggca gaagcgcuac     2220

cagcuccuga acgaccuggc gggcgugcuc cagguguccc ccaacuuccu gaaggacaag     2280

aucgggagcg acgacgaccu guucaucagc aaguggcucg ucgagcacau ccggggcuuc     2340

aagaaggccu gcgaggacuc ccugaagauc cagaaggaca accgcggccu gcucaaccac     2400

aagaucaaca ucgcccggaa caccaagggg aagugcgaga aggagaucuu caaccugauc     2460

ugcaagaucg agggcagcga ggacaagaag gggaacuaca agcacggccu ggccuacgag     2520

cucggggugc ugcuguucgg cgagccgaac gaggccucca agcccgaguu cgaccgcaag     2580

aucaagaagu ucaacagcau cuacuccuuc gcgcagaucc agcagaucgc cuucgccgag     2640

cggaagggca acgccaacac gugcgcgguc ugcagcgccg acaacgccca ccgcaugcag     2700

cagaucaaga ucaccgagcc cguggaggac aacaaggaca agaucauccu cuccgccaag     2760

gcccagcggc ugcccgcgau cccgacccgc aucguggacg gggccgucaa gaagauggcc     2820

accauccugg ccaagaacau cguggacgac aacuggcaga acaucaagca gguccucagc     2880

gccaagcacc agcugcacau ccccaucauc accgagucca acgcguucga guucgagccc     2940

gcccuggccg acgugaaggg caagagccuc aaggaccggc gcaagaaggc ccuggagcgg     3000

aucucccccg agaacaucuu caaggacaag aacaaccgca ucaaggaguu cgccaagggg     3060

aucagcgcgu acuccggcgc caaccugacc gacggggacu ucgacggcgc caaggaggag     3120

cucgaccaca ucaucccgcg gagccacaag aaguacggca cgcugaacga cgaggccaac     3180

cugaucugcg ugacccgcgg ggacaacaag aacaagggca accgcaucuu cugccuccgg     3240

gaccuggcgg acaacuacaa gcugaagcag uucgagacca ccgacgaccu cgagaucgag     3300

aagaagaucg ccgacacgau cugggacgcc aacaagaagg acuucaaguu cgggaacuac     3360

cgcuccuuca ucaaccugac gccccaggag cagaaggccu uccggcacgc ccuguuccuc     3420

gcggacgaga accccaucaa gcaggccgug auccgcgcca ucaacaaccg gaaccgcacc     3480

uucgucaacg gcacccagcg guacuucgcc gaggugcugg ccaacaacau cuaccugcgc     3540

gcgaagaagg agaaccucaa caccgacaag aucagcuucg acuacuucgg cauccccacc     3600

aucgggaacg gccgggggau cgccgagauc cgccagcugu acgagaaggu ggacuccgac     3660

auccaggccu acgccaaggg cgacaagccg caggccagcu acagccaccu gaucgacgcg     3720

augcucgccu ucugcaucgc cgccgacgag caccggaacg acggguccau cggccuggag     3780

aucgacaaga acuacagccu guacccccuc gacaagaaca ccggcgaggu cuucaccaag     3840

gacaucuucu cccagaucaa gaucaccgac aacgaguuca gcgacaagaa gcuggugcgc     3900

aagaaggcga ucgagggguu caacacgcac cggcagauga cccgcgacgg caucuacgcc     3960

gagaacuacc ugcccauccu cauccacaag gagcugaacg aggugcggaa gggguacacc     4020

uggaagaacu ccgaggagau caagaucuuc aagggcaaga aguacgacau ccagcagcug     4080

aacaaccucg ucuacugccu gaaguucgug gacaagccga ucagcaucga cauccagauc     4140

uccacccugg aggagcuccg caacauccug acgaccaaca acaucgccgc caccgccgag     4200

uacuacuaca ucaaccugaa gacccagaag cuccacgagu acuacaucga gaacuacaac     4260

acggcgcugg gguacaagaa guacagcaag gagauggagu uccugcgguc ccucgccuac     4320

cgcagcgagc gggugaagau caaguccauc gacgacguca agcagguccu ggacaaggac     4380

agcaacuuca ucaucggcaa gaucacccug cccuucaaga aggaguggca gcgccucuac     4440

cgggaguggc agaacaccac caucaaggac gacuacgagu uccugaaguc cuucuucaac     4500

gucaagagca ucacgaagcu gcacaagaag gugcgcaagg acuucucccu ccccaucagc     4560

accaacgagg gcaaguuccu ggugaagcgc aagaccuggg acaacaacuu caucuaccag     4620

auccugaacg acuccgacag ccgggccgac gggaccaagc ccuucauccc ggccuucgac     4680

aucuccaaga acgagaucgu ggaggccauc aucgacagcu ucacgagcaa gaacaucuuc     4740

uggcucccca agaacaucga gcugcagaag guggacaaca agaacaucuu cgcgaucgac     4800

accuccaagu gguucgaggu ggagaccccc agcgaccugc gcgacaucgg caucgccacc     4860

auccaguaca agaucgacaa caacucccgg cccaaggugc gcgucaagcu ggacuacgug     4920

aucgacgacg acagcaagau caacuacuuc augaaccacu cccuccucaa gagccgguac     4980

ccggacaagg ugcuggagau ccugaagcag uccacgauca ucgaguucga gagcuccggg     5040

uucaacaaga ccaucaagga gaugcugggc augaagcugg ccgggaucua caacgagacc     5100

agcaacaaca agcgccccgc cgccaccaag aaggcgggcc aggccaagaa gaagaaguaa     5160

ggacuaguga agggaacacc caaauuuaau ucagccuuaa gcacaauuaa uuaagaguga     5220

aacguaauug uacaagcagu uggucaccca ccauagggca ugaucaacac cgcaaccuuu     5280

ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu cagcuugcuu agauguucca     5340

aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa aaaaaggcca caaaaguucc     5400

cucucacuuu caguaaauaa aauaaaagca gcaacagaaa uaaagaaaua aaugaaauuc     5460

aaaaugaaau aaauauugug uugugcagca uuaaaaaauc aauaaaaauu aaaaaugagc     5520

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5580

aaaaaaaaaa agaauu                                                     5596


<210>  2348
<211>  3868
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2348
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagcg gaacuacauc      240

cugggccucg acaucgggau caccagcgug ggcuacggga ucaucgacua cgagacccgc      300

gacgugaucg acgccggcgu ccggcuguuc aaggaggcca acguggagaa caacgagggc      360

cgccggucca agcgcggggc ccggcgccug aagcggcgcc ggcgccaccg gauccagcgc      420

gugaagaagc uccuguucga cuacaaccug cucacggacc acagcgagcu guccggcauc      480

aaccccuacg aggcccgggu caaggggcug agccagaagc ucuccgagga ggaguucagc      540

gcggcccugc ugcaccucgc caagcgccgg ggcgugcaca acgugaacga ggucgaggag      600

gacaccggga acgagcuguc caccaaggag cagaucagcc gcaacuccaa ggcccuggag      660

gagaaguacg uggcggagcu ccagcuggag cggcugaaga aggacggcga ggugcgcggc      720

agcaucaacc gguucaagac cuccgacuac gucaaggagg ccaagcagcu ccugaaggug      780

cagaaggccu accaccagcu ggaccagagc uucaucgaca cguacaucga ccuccuggag      840

acccgccgca ccuacuacga ggggcccggc gagggguccc ccuucggcug gaaggacauc      900

aaggaguggu acgagaugcu gaugggccac ugcaccuacu ucccggagga gcuccggagc      960

gugaaguacg ccuacaacgc cgaccuguac aacgcgcuga acgaccucaa caaccugguc     1020

aucacgcgcg acgagaacga gaagcuggag uacuacgaga aguuccagau caucgagaac     1080

guguucaagc agaagaagaa gcccacccuc aagcagaucg ccaaggagau ccuggugaac     1140

gaggaggaca ucaaggggua ccgggucacc uccaccggca agcccgaguu cacgaaccug     1200

aagguguacc acgacaucaa ggacaucacc gcccgcaagg agaucaucga gaacgccgag     1260

cuccuggacc agaucgccaa gauccugacc aucuaccaga gcuccgagga cauccaggag     1320

gagcucacca accugaacag cgagcugacg caggaggaga ucgagcagau cuccaaccuc     1380

aagggguaca ccggcaccca caaccugagc cugaaggcga ucaaccucau ccuggacgag     1440

cuguggcaca ccaacgacaa ccagaucgcc aucuucaacc ggcucaagcu ggugcccaag     1500

aaggucgacc ugagccagca gaaggagauc ccgacgaccc ucguggacga cuucauccug     1560

ucccccgugg ucaagcgcag cuucauccag uccaucaagg ugaucaacgc caucaucaag     1620

aaguacgggc ugcccaacga caucaucauc gagcuggccc gggagaagaa cagcaaggac     1680

gcccagaaga ugaucaacga gaugcagaag cgcaaccggc agaccaacga gcgcaucgag     1740

gagaucaucc ggaccacggg caaggagaac gcgaaguacc ucaucgagaa gaucaagcug     1800

cacgacaugc aggagggcaa gugccuguac ucccucgagg ccaucccccu ggaggaccug     1860

cucaacaacc cguucaacua cgaggucgac cacaucaucc cccgcagcgu guccuucgac     1920

aacagcuuca acaacaaggu gcuggucaag caggaggaga acuccaagaa ggggaaccgg     1980

acccccuucc aguaccugag cuccagcgac uccaagauca gcuacgagac cuucaagaag     2040

cacauccuca accuggccaa gggcaagggg cgcaucucca agaccaagaa ggaguaccug     2100

cucgaggagc gggacaucaa ccgcuucagc gugcagaagg acuucaucaa ccggaaccug     2160

guggacacgc gcuacgccac ccggggccug augaaccucc ugcgcuccua cuuccgcguc     2220

aacaaccugg acgugaaggu caagagcauc aacgggggcu ucaccuccuu ccuccggcgc     2280

aaguggaagu ucaagaagga gcggaacaag ggcuacaagc accacgccga ggacgcgcug     2340

aucaucgcca acgccgacuu caucuucaag gaguggaaga agcuggacaa ggccaagaag     2400

gugauggaga accagauguu cgaggagaag caggcggaga gcaugcccga gaucgagacc     2460

gagcaggagu acaaggagau cuucaucacg ccgcaccaga ucaagcacau caaggacuuc     2520

aaggacuaca aguacagcca ccgcguggac aagaagccca accgggagcu caucaacgac     2580

acccuguacu ccacccgcaa ggacgacaag gggaacaccc ugaucgugaa caaccucaac     2640

ggccuguacg acaaggacaa cgacaagcug aagaagcuca ucaacaagag ccccgagaag     2700

cugcugaugu accaccacga cccccagacg uaccagaagc ucaagcugau cauggagcag     2760

uacggggacg agaagaaccc gcuguacaag uacuacgagg agaccggcaa cuaccucacc     2820

aaguacucca agaaggacaa cgggcccguc aucaagaaga ucaaguacua cggcaacaag     2880

cugaacgccc accuggacau caccgacgac uaccccaaca gccggaacaa gguggugaag     2940

cucucccuga agcccuaccg cuucgacguc uaccuggaca acggcgugua caaguucgug     3000

accgucaaga accucgacgu gaucaagaag gagaacuacu acgaggugaa cagcaagugc     3060

uacgaggagg ccaagaagcu gaagaagauc uccaaccagg ccgaguucau cgccagcuuc     3120

uacaacaacg accugaucaa gaucaacggg gagcucuacc gggucaucgg cgugaacaac     3180

gaccugcuga accgcaucga ggugaacaug aucgacauca cguaccggga guaccucgag     3240

aacaugaacg acaagcgccc gccccggauc aucaagacca ucgcguccaa gacccagagc     3300

aucaagaagu acuccaccga cauccugggg aaccuguacg aggucaagag caagaagcac     3360

ccccagauca ucaagaaggg caagcgcccc gccgccacga agaaggccgg ccaggccaag     3420

aagaagaagu aaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu     3480

aauuaagagu gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac     3540

accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc     3600

uuagauguuc caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc     3660

cacaaaaguu cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa     3720

uaaaugaaau ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa     3780

uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        3868


<210>  2349
<211>  4936
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2349
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgcuguu caacaagugc      240

aucaucauca gcaucaaccu cgacuucucc aacaaggaga agugcaugac caagcccuac      300

agcaucggcc uggacaucgg gaccaacucc gugggcuggg ccgugaucac ggacaacuac      360

aaggucccca gcaagaagau gaaggugcug gggaacaccu ccaagaagua caucaagaag      420

aaccuccugg gcgugcugcu guucgacagc ggcaucaccg ccgaggggcg gcgccugaag      480

cggaccgccc gccggcgcua cacgcggcgc cggaaccgca uccuguaccu ccaggagauc      540

uucuccaccg agauggccac ccuggacgac gcguucuucc agcggcugga cgacagcuuc      600

cucguccccg acgacaagcg cgacuccaag uacccgaucu ucggcaaccu gguggaggag      660

aagguguacc acgacgaguu ccccaccauc uaccaccucc ggaaguaccu cgccgacagc      720

acgaagaagg ccgaccugcg ccuggucuac cucgcccugg cgcacaugau caaguaccgg      780

gggcacuucc ugaucgaggg cgaguucaac uccaagaaca acgacaucca gaagaacuuc      840

caggacuucc ucgacaccua caacgccauc uucgagagcg accugucccu ggagaacagc      900

aagcagcucg aggagaucgu gaaggacaag aucuccaagc uggagaagaa ggaccgcauc      960

cugaagcugu uccccgggga gaagaacagc ggcaucuucu ccgaguuccu gaagcugauc     1020

gugggcaacc aggccgacuu ccggaagugc uucaaccucg acgagaaggc cagccugcac     1080

uucuccaagg agagcuacga cgaggaccug gagacccucc ugggguacau cggcgacgac     1140

uacagcgacg ucuuccugaa ggccaagaag cucuacgacg cgauccugcu guccggguuc     1200

cucaccguga cggacaacga gaccgaggcg ccccugagcu ccgccaugau caagcgcuac     1260

aacgagcaca aggaggaccu ggcccuccug aaggaguaca uccggaacau cagccugaag     1320

accuacaacg agguguucaa ggacgacacc aagaacggcu acgccggcua caucgacggg     1380

aagacgaacc aggaggacuu cuacgucuac cucaagaacc ugcuggccga guucgagggc     1440

gccgacuacu uccucgagaa gaucgaccgc gaggacuucc ugcggaagca gcgcaccuuc     1500

gacaacgggu ccaucccgua ccagauccac cuccaggaga ugcgcgccau ccuggacaag     1560

caggccaagu ucuaccccuu ccucgccaag aacaaggagc ggaucgagaa gauccugacc     1620

uuccgcaucc ccuacuacgu gggcccccug gcgcggggga acagcgacuu cgccuggucc     1680

auccgcaagc ggaacgagaa gaucaccccg uggaacuucg aggacgugau cgacaaggag     1740

agcuccgccg aggccuucau caaccgcaug acgagcuucg accucuaccu gcccgaggag     1800

aagguccugc ccaagcacuc ccuccuguac gagaccuuca acguguacaa cgagcugacc     1860

aaggugcggu ucaucgccga gagcaugcgc gacuaccagu uccucgacuc caagcagaag     1920

aaggacaucg uccggcugua cuucaaggac aagcgcaagg ugaccgacaa ggacaucauc     1980

gaguaccugc acgcgaucua cggcuacgac ggcaucgagc ucaaggggau cgagaagcag     2040

uucaacagcu cccugagcac guaccacgac cugcucaaca ucaucaacga caaggaguuc     2100

cuggacgacu ccagcaacga ggccaucauc gaggagauca uccacacccu gaccaucuuc     2160

gaggaccggg agaugaucaa gcagcgccuc uccaaguucg agaacaucuu cgacaagagc     2220

gugcugaaga agcugagccg gcgccacuac accggcuggg ggaagcucuc cgccaagcug     2280

aucaacggca uccgggacga gaaguccggg aacacgaucc uggacuaccu caucgacgac     2340

ggcaucagca accgcaacuu caugcagcug auccacgacg acgcccugag cuucaagaag     2400

aagauccaga aggcgcagau caucggcgac gaggacaagg ggaacaucaa ggaggucgug     2460

aagucccucc ccggcagccc ggccaucaag aaggggaucc ugcaguccau caagaucguc     2520

gacgagcugg ugaaggugau gggcgggcgg aagcccgaga gcaucgucgu ggagauggcc     2580

cgcgagaacc aguacaccaa ccagggcaag uccaacagcc agcagcggcu caagcgccug     2640

gagaaguccc ugaaggagcu cggcagcaag auccugaagg agaacauccc cgccaagcug     2700

uccaagaucg acaacaacgc ccuccagaac gaccgccugu accuguacua ccuccagaac     2760

gggaaggaca uguacaccgg cgacgaccug gacaucgacc ggcugagcaa cuacgacauc     2820

gaccacauca ucccccaggc guuccucaag gacaacucca ucgacaacaa ggugcugguc     2880

agcuccgcca gcaaccgcgg gaaguccgac gacuucccga gccuggaggu ggugaagaag     2940

cggaagaccu ucugguacca gcuccucaag agcaagcuga ucucccagcg caaguucgac     3000

aaccugacga aggccgagcg gggcggccug cugcccgagg acaaggccgg guucauccag     3060

cgccagcucg ucgagacccg gcagaucacc aagcacgugg cccgccugcu ggacgagaag     3120

uucaacaaca agaaggacga gaacaaccgg gcggugcgca ccgucaagau caucacgcuc     3180

aagagcaccc ugguguccca guuccggaag gacuucgagc uguacaaggu gcgcgagauc     3240

aacgacuucc accacgccca cgacgccuac cucaacgccg ucaucgccag cgcgcugcug     3300

aagaaguacc ccaagcucga gcccgaguuc guguacggcg acuacccgaa guacaacucc     3360

uuccgggagc gcaagagcgc caccgagaag guguacuucu acuccaacau caugaacauc     3420

uucaagaagu ccaucagccu ggccgacggg cgggucaucg agcgcccccu gaucgaggug     3480

aacgaggaga ccggcgagag cguguggaac aaggaguccg accucgccac cguccggcgc     3540

gugcugagcu acccccaggu gaacgucgug aagaaggugg aggagcagaa ccacgggcug     3600

gaccggggca agcccaaggg ccucuucaac gcgaaccugu ccagcaagcc gaagcccaac     3660

uccaacgaga accuggucgg ggccaaggag uaccucgacc ccaagaagua cggcggguac     3720

gccggcauca gcaacuccuu cgccgugcug gugaagggga cgaucgagaa gggcgccaag     3780

aagaagauca ccaacguccu ggaguuccag ggcaucagca uccucgaccg caucaacuac     3840

cggaaggaca agcugaacuu ccugcucgag aagggguaca aggacaucga gcugaucauc     3900

gagcugccga aguacagccu cuucgagcug uccgacggca gccgccgcau gcuggcgucc     3960

auccucagca ccaacaacaa gcgcggggag auccacaagg gcaaccagau cuuccugucc     4020

cagaaguucg ugaagcugcu cuaccacgcc aagcggauca gcaacaccau caacgagaac     4080

caccggaagu acguggagaa ccacaagaag gaguucgagg agcuguucua cuacauccug     4140

gaguucaacg agaacuacgu cggggccaag aagaacggca agcuccugaa cuccgccuuc     4200

cagagcuggc agaaccacuc caucgacgag cugugcagcu ccuucaucgg ccccacgggg     4260

agcgagcgca agggccucuu cgagcugacc ucccggggga gcgccgcgga cuucgaguuc     4320

cugggcguga agaucccccg cuaccgggac uacacccccu ccagccuccu gaaggacgcc     4380

acccugaucc accaguccgu gacggggcug uacgagaccc gcaucgaccu cgccaagcug     4440

ggcgagggca agcggccggc cgccaccaag aaggcggggc aggccaagaa gaagaaguaa     4500

ggacuaguga agggaacacc caaauuuaau ucagccuuaa gcacaauuaa uuaagaguga     4560

aacguaauug uacaagcagu uggucaccca ccauagggca ugaucaacac cgcaaccuuu     4620

ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu cagcuugcuu agauguucca     4680

aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa aaaaaggcca caaaaguucc     4740

cucucacuuu caguaaauaa aauaaaagca gcaacagaaa uaaagaaaua aaugaaauuc     4800

aaaaugaaau aaauauugug uugugcagca uuaaaaaauc aauaaaaauu aaaaaugagc     4860

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4920

aaaaaaaaaa agaauu                                                     4936


<210>  2350
<211>  4012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2350
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcguggua cgccagccug      240

auguccgccc accaccuccg ggugggcauc gacgugggga cccacagcgu cggccuggcc      300

acccugcgcg uggacgacca cgggacgccc aucgagcucc uguccgcccu gagccacauc      360

cacgacuccg gcgugggcaa ggaggggaag aaggaccacg acacccggaa gaagcucagc      420

ggcaucgcgc gccgggcccg ccggcugcug caccaccgcc ggacccagcu ccagcagcug      480

gacgaggucc ugcgcgaccu cggguucccc auccccaccc cgggcgaguu ccuggaccug      540

aacgagcaga cggaccccua ccgggugugg cgcgugcggg cccgccucgu cgaggagaag      600

cugcccgagg agcugcgggg gcccgccauc uccauggcgg ugcgccacau cgcccggcac      660

cgcggcuggc ggaacccgua cagcaaggug gagucccucc ugagccccgc cgaggagucc      720

cccuucauga aggcccugcg cgagcggauc cucgccacca ccggcgaggu ccuggacgac      780

gggaucaccc ccggccaggc gauggcccag guggcccuga cgcacaacau cagcaugcgc      840

gggccggagg gcauccucgg caagcugcac caguccgaca acgccaacga gauccgcaag      900

aucugcgccc ggcagggggu gagccccgac gucugcaagc agcugcuccg cgcgguguuc      960

aaggccgaca gcccccgggg cuccgccgug ucccgcgucg cccccgaccc gcugcccggg     1020

cagggcagcu uccggcgcgc ccccaagugc gaccccgagu uccagcgguu ccgcaucauc     1080

uccaucgugg cgaaccugcg gaucagcgag accaaggggg agaaccgccc gcucaccgcc     1140

gacgagcggc gccacguggu caccuuccug acggaggaca gccaggccga ccugaccugg     1200

guggacgugg ccgagaagcu cggcguccac cggcgcgacc ugcggggcac cgccgugcac     1260

accgacgacg gggagcgcuc cgcggcccgg ccgcccaucg acgccacgga ccgcaucaug     1320

cggcagacca agaucagcuc ccugaagacc uggugggagg aggccgacag cgagcagcgc     1380

ggcgcgauga uccgcuaccu cuacgaggac cccaccgacu ccgagugcgc cgagaucauc     1440

gccgagcugc ccgaggagga ccaggccaag cuggacagcc uccaccuccc cgccgggcgg     1500

gcggccuacu cccgcgagag ccugacggcc cuguccgacc acaugcuggc caccaccgac     1560

gaccugcacg aggcccggaa gcgccucuuc ggcgucgacg acagcugggc gccgcccgcc     1620

gaggccauca acgcccccgu ggggaacccc uccguggacc ggacccugaa gaucgucggc     1680

cgcuaccuga gcgccgugga guccaugugg ggcacgcccg aggugaucca cgucgagcac     1740

gugcgggacg gguucaccag cgagcgcaug gcggacgagc gggacaaggc caaccgccgg     1800

cgcuacaacg acaaccagga ggccaugaag aagauccagc gggacuacgg caaggagggg     1860

uacaucuccc gcggcgacau cgugcggcuc gacgcccugg agcugcaggg gugcgcgugc     1920

cuguacugcg gcaccaccau cggcuaccac acgugccagc ucgaccacau cgucccgcag     1980

gccgggcccg gcagcaacaa ccgccggggg aaccuggugg ccgugugcga gcgcugcaac     2040

cgguccaaga gcaacacccc cuucgccguc ugggcccaga agugcggcau cccccacgug     2100

ggcgugaagg aggcgaucgg gcgcguccgg ggcuggcgca agcagacccc gaacaccucc     2160

agcgaggacc ugacgcgccu caagaaggag gugaucgccc ggcugcgccg gacccaggag     2220

gaccccgaga ucgacgagcg cagcauggag uccguggccu ggauggccaa cgagcugcac     2280

caccggaucg ccgcggccua ccccgagacc accgucaugg uguaccgcgg gagcaucacg     2340

gccgccgccc ggaaggcggc cggcaucgac ucccgcauca accucaucgg ggagaagggc     2400

cggaaggacc gcaucgaccg gcgccaccac gccguggacg ccagcgucgu ggcgcugaug     2460

gaggccuccg uggccaagac ccuggccgag cggagcuccc uccgcggcga gcagcggcug     2520

accgggaagg agcagaccug gaagcaguac accggcagca cggucggggc ccgcgagcac     2580

uucgagaugu ggcggggcca caugcugcac cucaccgagc uguucaacga gcgccuggcg     2640

gaggacaagg uguacgugac ccagaacauc cggcuccgcc uguccgacgg gaacgcccac     2700

accgucaacc ccagcaagcu ggugucccac cgccucggcg acggccugac ggugcagcag     2760

aucgaccggg ccugcacccc ggcccugugg ugcgcccuca cccgcgagaa ggacuucgac     2820

gagaagaacg ggcugcccgc gcgggaggac cgcgccaucc ggguccacgg ccacgagauc     2880

aagagcuccg acuacaucca gguguucagc aagcgcaaga agaccgacuc cgaccgggac     2940

gagacgcccu ucggggccau cgccgugcgc ggcggguucg ucgagaucgg ccccagcauc     3000

caccacgccc ggaucuaccg cguggagggc aagaagccgg uguacgcgau gcugcggguc     3060

uucacccacg accuccuguc ccagcgccac ggggaccugu ucagcgccgu gaucccgccc     3120

cagagcaucu ccaugcggug cgccgagccc aagcuccgca aggccaucac caccggcaac     3180

gccacguacc uggggugggu ggucgugggc gacgagcugg agaucaacgu ggacagcuuc     3240

accaaguacg cgaucgggcg guuccucgag gacuucccca acaccacccg cuggcggauc     3300

ugcggcuacg acacgaacuc caagcugacc cugaagccca ucguccucgc cgccgagggc     3360

cuggagaacc ccagcuccgc cgugaacgag aucguggagc ugaaggggug gcgcgucgcg     3420

aucaacgugc ucaccaaggu gcaccccacc gucgugcggc gcgacgcccu gggccggccg     3480

cgcuacagcu cccgcagcaa ccugcccacg uccuggacca ucgagaagcg gcccgccgcc     3540

accaagaagg ccgggcaggc gaagaagaag aaguaaggac uagugaaggg aacacccaaa     3600

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu     3660

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa     3720

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu     3780

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     3840

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     3900

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     3960

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             4012


<210>  2351
<211>  4873
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2351
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaccaa gcccuacagc      240

aucggccugg acaucgggac caacuccgug ggcugggccg ugacgaccga caacuacaag      300

guccccagca agaagaugaa ggugcucggg aacaccucca agaaguacau caagaagaac      360

cugcugggcg ugcuccuguu cgacagcggc aucaccgccg aggggcggcg ccugaagcgg      420

acggcccgcc ggcgcuacac ccggcgccgg aaccgcaucc ucuaccugca ggagaucuuc      480

uccaccgaga uggccacccu ggacgacgcg uucuuccagc ggcucgacga cagcuuccug      540

guccccgacg acaagcgcga cuccaaguac ccgaucuucg gcaaccuggu ggaggagaag      600

gccuaccacg acgaguuccc cacgaucuac caccuccgga aguaccuggc cgacagcacc      660

aagaaggccg accugcgccu cguguaccug gcgcuggccc acaugaucaa guaccggggg      720

cacuuccuca ucgagggcga guucaacucc aagaacaacg acauccagaa gaacuuccag      780

gacuuccugg acaccuacaa cgccaucuuc gagagcgacc ugucccucga gaacagcaag      840

cagcuggagg agaucgucaa ggacaagauc uccaagcugg agaagaagga ccgcauccuc      900

aagcuguucc ccggggagaa gaacagcggc aucuucuccg aguuccugaa gcucaucgug      960

ggcaaccagg ccgacuuccg gaagugcuuc aaccuggacg agaaggccag ccugcacuuc     1020

uccaaggaga gcuacgacga ggaccucgag acccugcugg gguacaucgg cgacgacuac     1080

uccgacgugu uccucaaggc gaagaagcug uacgacgcca uccugcucag cggguuccug     1140

acggucaccg acaacgagac cgaggcgccc cugagcuccg ccaugaucaa gcgcuacaac     1200

gagcacaagg aggaccucgc ccugcugaag gaguacaucc ggaacaucag ccucaagacc     1260

uacaacgagg uguucaagga cgacacgaag aacggcuacg ccggcuacau cgacgggaag     1320

accaaccagg aggacuucua cguguaccug aagaagcugc ucgccgaguu cgagggcgcc     1380

gacuacuucc uggagaagau cgaccgcgag gacuuccugc ggaagcagcg caccuucgac     1440

aacgggucca ucccguacca gauccaccuc caggagaugc gcgccauccu ggacaagcag     1500

gccaaguucu accccuuccu ggcgaagaac aaggagcgga ucgagaagau ccugaccuuc     1560

cgcauccccu acuacgucgg cccccucgcc cgggggaaca gcgacuucgc cugguccauc     1620

cgcaagcgga acgagaagau cacgccgugg aacuucgagg acgugaucga caaggagagc     1680

uccgccgagg ccuucaucaa ccgcaugacc agcuucgacc uguaccugcc cgaggagaag     1740

gugcucccca agcacucccu gcuguacgag accuucaacg ucuacaacga gcucaccaag     1800

gugcgguuca ucgcggagag caugcgcgac uaccaguucc uggacuccaa gcagaagaag     1860

gacaucgugc ggcuguacuu caaggacaag cgcaagguca cggacaagga caucaucgag     1920

uaccuccacg ccaucuacgg cuacgacggc aucgagcuga aggggaucga gaagcaguuc     1980

aacagcuccc ugagcaccua ccacgaccuc cugaacauca ucaacgacaa ggaguuccug     2040

gacgacucca gcaacgaggc caucaucgag gagaucaucc acacccucac caucuucgag     2100

gaccgggaga ugaucaagca gcgccugucc aaguucgaga acaucuucga caagagcgug     2160

cugaagaagc ucucccggcg ccacuacacg ggcuggggga agcugagcgc caagcugauc     2220

aacggcaucc gggacgagaa guccgggaac accauccucg acuaccugau cgacgacggc     2280

aucagcaacc gcaacuucau gcagcugauc cacgacgacg cgcucagcuu caagaagaag     2340

auccagaagg cccagaucau cggcgacgag gacaagggga acaucaagga gguggucaag     2400

ucccugcccg gcagcccggc caucaagaag gggauccugc aguccaucaa gaucguggac     2460

gagcucguca aggugauggg cgggcggaag cccgagagca ucguggucga gauggcccgc     2520

gagaaccagu acaccaacca gggcaagucc aacagccagc agcggcugaa gcgccuggag     2580

aagucccuca aggagcuggg cagcaagauc cugaaggaga acauccccgc caagcucucc     2640

aagaucgaca acaacgcgcu gcagaacgac cgccuguacc ucuacuaccu gcagaacggg     2700

aaggacaugu acaccggcga cgaccuggac aucgaccggc ucagcaacua cgacaucgac     2760

cacaucaucc cccaggccuu ccugaaggac aacuccaucg acaacaaggu gcuggugagc     2820

uccgccagca accgcgggaa guccgacgac gucccgagcc ucgagguggu gaagaagcgg     2880

aagacguucu gguaccagcu gcucaagagc aagcugaucu cccagcgcaa guucgacaac     2940

cugaccaagg ccgagcgggg cggccugagc cccgaggaca aggccggguu cauccagcgc     3000

cagcucgucg agacccggca gaucaccaag cacguggcgc gccugcugga cgagaaguuc     3060

aacaacaaga aggacgagaa caaccgggcc gugcgcacgg ucaagaucau cacccucaag     3120

uccacccugg ugagccaguu ccggaaggac uucgagcugu acaaggugcg cgagaucaac     3180

gacuuccacc acgcccacga cgccuaccuc aacgccgucg uggcguccgc ccugcugaag     3240

aaguacccca agcucgagcc cgaguucgug uacggcgacu acccgaagua caacagcuuc     3300

cgggagcgca aguccgccac cgagaagguc uacuucuaca gcaacaucau gaacaucuuc     3360

aagaagucca ucagccuggc cgacgggcgg gugaucgagc gcccccugau cgaggugaac     3420

gaggagaccg gcgaguccgu cuggaacaag gagagcgacc ucgcgacggu gcggcgcgug     3480

cuguccuacc cccaggucaa cguggugaag aaggucgagg agcagaacca cgggcuggac     3540

cggggcaagc ccaagggccu cuucaacgcc aaccugagcu ccaagccgaa gcccaacagc     3600

aacgagaacc uggugggggc caaggaguac cucgacccca agaaguacgg cggguacgcc     3660

ggcaucucca acagcuucac cgugcugguc aaggggacca ucgagaaggg cgccaagaag     3720

aagaucacca acgugcugga guuccagggc aucuccaucc ucgaccgcau caacuaccgg     3780

aaggacaagc ugaacuuccu gcucgagaag ggguacaagg acaucgagcu gaucaucgag     3840

cugccgaagu acagccucuu cgagcugagc gacggcuccc gccgcaugcu ggcgagcauc     3900

cucuccacga acaacaagcg cggggagauc cacaagggca accagaucuu ccugagccag     3960

aaguucguga agcugcucua ccacgccaag cggaucucca acaccaucaa cgagaaccac     4020

cggaaguacg ucgagaacca caagaaggag uucgaggagc uguucuacua cauccuggag     4080

uucaacgaga acuacguggg ggccaagaag aacggcaagc uccugaacag cgccuuccag     4140

uccuggcaga accacagcau cgacgagcug ugcuccagcu ucaucggccc caccgggucc     4200

gagcgcaagg gccucuucga gcugaccagc cggggguccg ccgcggacuu cgaguuccug     4260

ggcgugaaga ucccccgcua ccgggacuac acgcccagcu cccuccugaa ggacgccacc     4320

cugauccacc agagcgucac cgggcucuac gagacccgca ucgaccuggc caagcugggc     4380

gagggcaagc ggccggccgc cacgaagaag gcggggcagg ccaagaagaa gaaguaagga     4440

cuagugaagg gaacacccaa auuuaauuca gccuuaagca caauuaauua agagugaaac     4500

guaauuguac aagcaguugg ucacccacca uagggcauga ucaacaccgc aaccuuuccu     4560

uuuuccccca gugauucuga aaaaccccuc uucccuucag cuugcuuaga uguuccaaau     4620

uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa aaggccacaa aaguucccuc     4680

ucacuuucag uaaauaaaau aaaagcagca acagaaauaa agaaauaaau gaaauucaaa     4740

augaaauaaa uauuguguug ugcagcauua aaaaaucaau aaaaauuaaa aaugagcaag     4800

aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4860

aaaaaaaaga auu                                                        4873


<210>  2352
<211>  3955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2352
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggccgc cuucaagccc      240

aacagcauca acuacauccu gggccucgac aucgggaucg ccuccguggg cugggccaug      300

guggagaucg acgaggagga gaaccccauc cggcugaucg accugggggu ccgcguguuc      360

gagcgggcgg aggugcccaa gaccggcgac agccucgcca uggcccgccg gcuggcccgc      420

uccguccggc gccugacccg gcgccgggcg caccgccucc ugcggacgcg ccggcugcuc      480

aagcgcgagg gcgugcugca ggccgccaac uucgacgaga acgggcugau caagagccuc      540

ccgaacaccc ccuggcagcu gcgggccgcc gcgcuggacc gcaagcucac gccccuggag      600

ugguccgccg ugcugcucca ccugaucaag caccggggcu accugagcca gcgcaagaac      660

gagggggaga ccgccgacaa ggagcucggc gcccugcuga agggggucgc cggcaacgcg      720

cacgcccucc agaccggcga cuuccggacc cccgccgagc uggcccugaa caaguucgag      780

aaggaguccg ggcacauccg caaccagcgc agcgacuacu cccacaccuu cagccggaag      840

gaccuccagg ccgagcugau ccuccuguuc gagaagcaga aggaguucgg caacccgcac      900

guguccgggg gccugaagga gggcaucgag acccugcuca ugacgcagcg ccccgcgcug      960

agcggggacg ccgugcagaa gaugcugggc cacugcaccu ucgagcccgc cgagcccaag     1020

gccgccaaga acaccuacac cgcggagcgg uucaucuggc ucacgaagcu gaacaaccug     1080

cgcauccucg agcagggguc cgagcggccg cugaccgaca ccgagcgcgc cacccugaug     1140

gacgagcccu accgcaagag caagcucacg uacgcccagg cccggaagcu gcugggccuc     1200

gaggacaccg cguucuucaa ggggcugcgg uacggcaagg acaacgccga ggccuccacc     1260

cugauggaga ugaaggccua ccacgccauc agccgcgcgc ucgagaagga gggccugaag     1320

gacaagaagu ccccgcugaa ccugagcccc gagcuccagg acgagaucgg gaccgccuuc     1380

ucccuguuca agacggacga ggacaucacc ggccggcuga aggaccgcau ccagccggag     1440

auccucgagg cccugcugaa gcacaucagc uucgacaagu ucguccagau cagccucaag     1500

gcccugcggc gcaucgugcc ccugauggag caggggaagc gguacgacga ggccugcgcg     1560

gagaucuacg gcgaccacua cgggaagaag aacaccgagg agaagaucua ccucccgccc     1620

auccccgccg acgagauccg caaccccgug guccugcggg cccuguccca ggcccgcaag     1680

gugaucaacg gcgugguccg gcgcuacggc agccccgccc gcauccacau cgagaccgcg     1740

cgggaggugg ggaaguccuu caaggaccgc aaggagaucg agaagcggca ggaggagaac     1800

cgcaaggacc gggagaaggc cgccgccaag uuccgcgagu acuuccccaa cuucgugggc     1860

gagcccaaga gcaaggacau ccucaagcug cggcuguacg agcagcagca cgggaagugc     1920

cucuacuccg gcaaggagau caaccugggg cgccugaacg agaagggcua cgucgagauc     1980

gaccacgcgc uccccuucag ccggacgugg gacgacuccu ucaacaacaa ggugcuggug     2040

cugggcagcg agaaccagaa caaggggaac cagacccccu acgaguacuu caacggcaag     2100

gacaacuccc gcgaguggca ggaguucaag gcccgggucg agaccagccg cuucccccgg     2160

uccaagaagc agcgcauccu ccugcagaag uucgacgagg acggguucaa ggagcggaac     2220

cugaacgaca cccgcuacgu gaaccgguuc cucugccagu ucgucgccga ccgcaugcgg     2280

cugacgggca agggcaagaa gcgcguguuc gccagcaacg ggcagaucac caaccugcuc     2340

cggggcuucu gggggcugcg caaggugcgc gccgagaacg accggcacca cgcgcuggac     2400

gccgucgugg uggccugcuc caccgucgcc augcagcaga agaucacccg cuucgugcgg     2460

uacaaggaga ugaacgccuu cgacggcaag acgaucgaca aggagaccgg ggaggugcuc     2520

caccagaaga cccacuuccc gcagcccugg gaguucuucg cgcaggaggu caugauccgc     2580

guguucggca agcccgacgg caagccggag uucgaggagg ccgacacccu ggagaagcug     2640

cggacgcucc uggccgagaa gcugagcucc cgccccgagg ccgugcacga guacgucacg     2700

ccccucuucg ugagccgggc ccccaaccgc aagauguccg ggcagggcca cauggagacc     2760

gucaagagcg cgaagcggcu ggacgagggg guguccgugc ugcgcgugcc gcucacccag     2820

cugaagcuga aggaccucga gaagaugguc aaccgggagc gcgagcccaa gcuguacgag     2880

gcccugaagg cccggcucga ggcccacaag gacgaccccg cgaaggccuu cgccgagccc     2940

uucuacaagu acgacaaggc cggcaaccgc acccagcagg ucaaggccgu gcggguggag     3000

cagguccaga agaccggggu gugggugcgc aaccacaacg gcaucgcgga caacgccacc     3060

auggugcggg uggacgucuu cgagaagggc gacaaguacu accuggugcc gaucuacagc     3120

uggcaggucg ccaaggggau ccugcccgac cgcgccgugg ugcagggcaa ggacgaggag     3180

gacuggcagc ucaucgacga cagcuucaac uucaaguucu cccugcaccc caacgaccug     3240

guggagguca ucaccaagaa ggcccggaug uucggguacu ucgcgagcug ccaccgcggc     3300

accgggaaca ucaacauccg cauccacgac cucgaccaca agaucggcaa gaacggcauc     3360

cuggagggga ucggcgugaa gacggcccug uccuuccaga aguaccagau cgacgagcuc     3420

gggaaggaga uccggcccug ccgccugaag aagcggccgc ccgugcgcaa gcggcccgcc     3480

gccaccaaga aggccggcca ggcgaagaag aagaaguaag gacuagugaa gggaacaccc     3540

aaauuuaauu cagccuuaag cacaauuaau uaagagugaa acguaauugu acaagcaguu     3600

ggucacccac cauagggcau gaucaacacc gcaaccuuuc cuuuuucccc cagugauucu     3660

gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa auuuaguaag cuuaaggcgg     3720

ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc ucucacuuuc aguaaauaaa     3780

auaaaagcag caacagaaau aaagaaauaa augaaauuca aaaugaaaua aauauugugu     3840

ugugcagcau uaaaaaauca auaaaaauua aaaaugagca agaucuaaaa aaaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu          3955


<210>  2353
<211>  4711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2353
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagaa gcccuacacc      240

aucggccugg acaucgggac caacagcgug ggcugggccg ugcucacgga ccaguacgac      300

cuggucaagc ggaagaugaa gaucgccggg gacuccgaga agaagcagau caagaagaac      360

uucuggggcg ugcgccuguu cgacgagggc cagaccgccg ccgaccggcg cauggcgcgg      420

accgcccgcc ggcgcaucga gcggcgccgg aaccgcauca gcuaccucca ggggaucuuc      480

gccgaggaga uguccaagac cgacgccaac uucuucugcc ggcugagcga cuccuucuac      540

guggacaacg agaagcgcaa cagccggcac cccuucuucg cgacgaucga ggaggagguc      600

gaguaccaca agaacuaccc caccaucuac caccuccgcg aggagcuggu gaacuccagc      660

gagaaggccg accugcggcu gguguaccuc gcccuggccc acaucaucaa guaccgcggc      720

aacuuccuga ucgagggggc ccucgacacc cagaacaccu ccgucgacgg caucuacaag      780

caguucaucc agacguacaa ccagguguuc gcgagcggga ucgaggacgg cucccugaag      840

aagcuggagg acaacaagga cguggccaag auccucgucg agaaggugac ccggaaggag      900

aagcuggagc gcauccugaa gcucuacccg ggcgagaagu ccgccgggau guucgcccag      960

uucaucagcc ugaucguggg cagcaagggg aacuuccaga agcccuucga ccugaucgag     1020

aaguccgaca ucgagugcgc caaggacagc uacgaggagg accucgaguc ccugcuggcg     1080

cucaucggcg acgaguacgc cgagcuguuc gucgccgcca agaacgccua cagcgcggug     1140

gugcugucca gcaucaucac cgucgccgag accgagacga acgccaagcu cagcgccucc     1200

augaucgagc gguucgacac ccacgaggag gaccugggcg agcugaaggc cuucaucaag     1260

cuccaccucc ccaagcacua cgaggagauc uucagcaaca ccgagaagca cggguacgcg     1320

ggcuacaucg acgggaagac caagcaggcc gacuucuaca aguacaugaa gaugacgcug     1380

gagaacaucg agggcgccga cuacuucauc gccaagaucg agaaggagaa cuuccugcgc     1440

aagcagcgca ccuucgacaa cggggcgauc ccccaccagc ugcaccugga ggagcucgag     1500

gccauccugc accagcaggc caaguacuac ccguuccuga aggagaacua cgacaagauc     1560

aagucccucg ugaccuuccg gauccccuac uucgugggcc cccuggccaa cggccagagc     1620

gaguucgccu ggcugacccg caaggcggac ggggagaucc ggcccuggaa caucgaggag     1680

aaggucgacu ucggcaaguc cgccguggac uucaucgaga agaugacgaa caaggacacc     1740

uaccucccga aggagaacgu gcugcccaag cacagccugu gcuaccagaa guaccucguc     1800

uacaacgagc ugaccaaggu gcgcuacauc aacgaccagg ggaagaccuc cuacuucagc     1860

ggccaggaga aggagcagau cuucaacgac cuguucaagc agaagcggaa ggucaagaag     1920

aaggaccucg agcuguuccu gcgcaacaug ucccacgugg agagccccac gaucgagggg     1980

cuggaggacu ccuucaacag cuccuacagc accuaccacg accuccugaa ggugggcauc     2040

aagcaggaga uccuggacaa ccccgucaac accgagaugc ucgagaacau cgugaagauc     2100

cugaccgugu ucgaggacaa gcggaugauc aaggagcagc ugcagcaguu cuccgacguc     2160

cucgacggcg uggugcugaa gaagcuggag cgccggcacu acacggggug gggccgccuc     2220

agcgccaagc ugcugauggg gauccgggac aagcaguccc accucaccau ccuggacuac     2280

cugaugaacg acgacggccu caaccgcaac cugaugcagc ugaucaacga cagcaaccuc     2340

uccuucaaga gcaucaucga gaaggagcag gucaccaccg ccgacaagga cauccagagc     2400

aucguggccg accuggcggg guccccggcc aucaagaagg gcauccugca gagccucaag     2460

aucguggacg agcuggucuc cgugaugggc uacccgcccc agacgaucgu ggucgagaug     2520

gcccgggaga accagaccac cgggaagggc aagaacaaca gccgcccccg guacaagucc     2580

cuggagaagg ccaucaagga guucgggagc cagauccuca aggagcaccc caccgacaac     2640

caggagcugc gcaacaaccg gcuguaccuc uacuaccugc agaacggcaa ggacauguac     2700

acgggccagg accuggacau ccacaaccuc uccaacuacg acaucgacca caucgugccc     2760

cagagcuuca ucaccgacaa cuccaucgac aaccuggugc ugaccagcuc cgccgggaac     2820

cgcgagaagg gcgacgacgu cccgccccuc gagaucgugc ggaagcgcaa gguguucugg     2880

gagaagcugu accaggggaa ccugaugagc aagcgcaagu ucgacuaccu caccaaggcg     2940

gagcggggcg ggcugaccga ggccgacaag gcccgcuuca uccaccggca gcuggucgag     3000

acgcgccaga ucaccaagaa cguggccaac auccuccacc agcgguucaa cuacgagaag     3060

gacgaccacg gcaacaccau gaagcagguc cgcaucguga cccugaaguc cgcgcuggug     3120

agccaguucc ggaagcaguu ccagcucuac aaggugcgcg acgucaacga cuaccaccac     3180

gcccacgacg ccuaccugaa cggcguggug gccaacacgc ugcucaaggu cuacccccag     3240

cuggagcccg aguucgugua cggggacuac caccaguucg acugguucaa ggccaacaag     3300

gcgaccgcca agaagcaguu cuacaccaac aucaugcugu ucuucgccca gaaggaccgg     3360

aucaucgacg agaacggcga gauccucugg gacaagaagu accuggacac cgugaagaag     3420

gucauguccu accgccagau gaacaucgug aagaagacgg agauccagaa gggggaguuc     3480

agcaaggcca ccaucaagcc caagggcaac uccagcaagc ugaucccccg gaagaccaac     3540

ugggacccga ugaaguacgg gggccucgac agccccaaca uggccuacgc gguggucauc     3600

gaguacgcca agggcaagaa caagcuggug uucgagaaga agaucauccg cgugaccauc     3660

auggagcgga aggccuucga gaaggacgag aaggccuucc uggaggagca gggguaccgc     3720

cagcccaagg uccucgccaa gcugccgaag uacacgcugu acgagugcga ggagggccgg     3780

cgccggaugc ucgcguccgc caacgaggcc cagaagggga accagcaggu ccugcccaac     3840

caccugguga cccuccugca ccacgccgcg aacugcgagg ugagcgacgg caagucccug     3900

gacuacaucg agagcaaccg cgagauguuc gccgagcucc uggcccacgu guccgaguuc     3960

gccaagcggu acacccuggc cgaggcgaac cucaacaaga ucaaccagcu guucgagcag     4020

aacaaggagg gggacaucaa ggccaucgcc cagagcuucg uggaccugau ggccuucaac     4080

gccaugggcg cgcccgccuc cuucaaguuc uucgagacca cgaucgagcg caagcgguac     4140

aacaaccuca aggagcugcu gaacagcacc aucaucuacc aguccaucac cggccucuac     4200

gagagccgca agcgccugga cgacaagcgg cccgccgcca ccaagaaggc cgggcaggcg     4260

aagaagaaga aguaaggacu agugaaggga acacccaaau uuaauucagc cuuaagcaca     4320

auuaauuaag agugaaacgu aauuguacaa gcaguugguc acccaccaua gggcaugauc     4380

aacaccgcaa ccuuuccuuu uucccccagu gauucugaaa aaccccucuu cccuucagcu     4440

ugcuuagaug uuccaaauuu aguaagcuua aggcggccua cagaagaaaa agaaaaaaaa     4500

ggccacaaaa guucccucuc acuuucagua aauaaaauaa aagcagcaac agaaauaaag     4560

aaauaaauga aauucaaaau gaaauaaaua uuguguugug cagcauuaaa aaaucaauaa     4620

aaauuaaaaa ugagcaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                                    4711


<210>  2354
<211>  4744
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2354
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagaa gcccuacagc      240

aucggccugg acaucgggac caacuccgug ggcugggccg uggucaccga cgacuacaag      300

gugcccgcca agaagaugaa ggugcucggg aacacggaca agagccacau cgagaagaac      360

cugcugggcg cccuccuguu cgacuccggc aacaccgccg aggaccggcg ccugaagcgg      420

accgcgcgcc ggcgcuacac ccggcgccgg aaccgcaucc ucuaccugca ggagaucuuc      480

agcgaggaga uggggaaggu cgacgacucc uucuuccacc ggcuggagga cagcuuccuc      540

gugacggagg acaagcgcgg cgagcggcac cccaucuucg ggaaccugga ggaggaggug      600

aaguaccacg agaacuuccc gaccaucuac caccuccgcc aguaccuggc cgacaacccc      660

gagaaggucg accugcggcu gguguaccuc gcccuggccc acaucaucaa guuccgcggc      720

cacuuccuga ucgaggggaa guucgacacc cggaacaacg acgugcagcg ccucuuccag      780

gaguuccugg cggucuacga caacaccuuc gagaacucca gccugcagga gcagaacguc      840

cagguggagg agauccucac ggacaagauc uccaagagcg ccaagaagga ccgggugcug      900

aagcuguucc ccaacgagaa guccaacggc cgcuucgccg aguuccucaa gcugaucgug      960

ggcaaccagg ccgacuucaa gaagcacuuc gagcuggagg agaaggcccc gcuccaguuc     1020

agcaaggaca ccuacgagga ggagcuggag gugcugcucg cgcagaucgg ggacaacuac     1080

gccgagcugu uccuguccgc caagaagcuc uacgacagca uccugcuguc cggcauccuc     1140

accgucaccg acguggggac gaaggccccg cugagcgccu ccaugaucca gcgguacaac     1200

gagcaccaga uggaccuggc gcagcucaag caguucaucc gccagaagcu gagcgacaag     1260

uacaacgagg uguucuccga cgucagcaag gacggcuacg ccggcuacau cgacgggaag     1320

accaaccagg aggccuucua caaguaccug aagggccucc ugaacaagau cgaggggagc     1380

ggcuacuucc uggacaagau cgagcgcgag gacuuccucc ggaagcagcg caccuucgac     1440

aacgggucca ucccccacca gauccaccuc caggagaugc gggccaucau ccgccggcag     1500

gccgaguucu accccuuccu ggcggacaac caggaccgca ucgagaagcu gcugaccuuc     1560

cggauccccu acuacguggg cccgcuggcc cgcggcaaga gcgacuucgc cuggcugucc     1620

cgcaagagcg ccgacaagau cacgcccugg aacuucgacg agaucgugga caaggagucc     1680

agcgccgagg cguucaucaa ccggaugacc aacuacgacc ucuaccugcc caaccagaag     1740

guccugccca agcacucccu ccuguacgag aaguucaccg uguacaacga gcugaccaag     1800

gucaaguaca agacggagca ggggaagacc gccuucuucg acgccaacau gaagcaggag     1860

aucuucgacg gcguguucaa gguguaccgg aaggucacca aggacaagcu cauggacuuc     1920

cuggagaagg aguucgacga guuccgcauc guggaccuga ccgggcucga caaggagaac     1980

aagguguuca acgccagcua cggcacguac cacgaccugu gcaagauccu ggacaaggac     2040

uuccucgaca acuccaagaa cgagaagauc cuggaggaca ucguccugac ccucacccug     2100

uucgaggacc gggagaugau ccgcaagcgg cuggagaacu acagcgaccu ccugaccaag     2160

gagcagguca agaagcugga gcgccggcac uacacggggu ggggccgccu cuccgcggag     2220

cugauccacg gcauccggaa caaggagagc cgcaagacca uccuggacua ccucaucgac     2280

gacgggaacu ccaaccgcaa cuucaugcag cugaucaacg acgacgcccu gagcuucaag     2340

gaggagaucg ccaaggccca ggugaucggc gagaccgaca accucaacca gguggugucc     2400

gacaucgccg ggagcccggc gaucaagaag ggcauccugc agucccugaa gaucguggac     2460

gagcucguca agaucauggg gcaccagccc gagaacaucg ugguggagau ggcccgggag     2520

aaccaguuca ccaaccaggg ccgccggaac agccagcagc gccugaaggg ccugacggac     2580

uccaucaagg aguucgggag ccagauccuc aaggagcacc ccgucgagaa cagccagcug     2640

cagaacgacc ggcuguuccu cuacuaccug cagaacggcc gcgacaugua caccggggag     2700

gagcuggaca ucgacuaccu cucccaguac gacaucgacc acaucauccc ccaggccuuc     2760

aucaaggaca acagcaucga caaccgggug cugaccucca gcaaggagaa ccgcggcaag     2820

uccgacgacg ugccgagcaa ggacgucgug cggaagauga aguccuacug gagcaagcug     2880

cucuccgcca agcugaucac ccagcgcaag uucgacaacc ugacgaaggc cgagcggggc     2940

gggcucaccg acgacgacaa ggcgggcuuc aucaagcgcc agcuggugga gacccggcag     3000

aucaccaagc acgucgcccg cauccuggac gagcgguuca acaccgagac ggacgagaac     3060

aacaagaaga uccgccaggu gaagaucgug acccucaaga gcaaccuggu cuccaacuuc     3120

cggaaggagu ucgagcugua caaggugcgc gagaucaacg acuaccacca cgcccacgac     3180

gccuaccuca acgccgugau cgggaaggcg cugcugggcg ucuaccccca gcucgagccc     3240

gaguucgugu acggggacua cccccacuuc cacggccaca aggagaacaa ggccaccgcc     3300

aagaaguucu ucuacagcaa caucaugaac uucuucaaga aggacgacgu gcggaccgac     3360

aagaacggcg agaucaucug gaagaaggac gagcacaucu ccaacaucaa gaagguccug     3420

agcuaccccc aggugaacau cgugaagaag gucgaggagc agaccggggg cuucuccaag     3480

gagagcaucc ugcccaaggg gaacuccgac aagcucaucc cccgcaagac gaagaaguuc     3540

uacugggaca ccaagaagua cggcggguuc gacagcccca ucguggccua cagcauccug     3600

gugaucgcgg acaucgagaa gggcaagucc aagaagcuga agaccgucaa ggcccucgug     3660

ggcgugacga ucauggagaa gaugaccuuc gagcgggacc cggucgccuu ccuggagcgc     3720

aagggguacc gcaacgugca ggaggagaac aucaucaagc ugcccaagua cagccucuuc     3780

aagcuggaga acggccggaa gcgccugcuc gccuccgccc gggagcugca gaaggggaac     3840

gagaucgugc ugcccaacca ccucggcacc cugcuguacc acgcgaagaa cauccacaag     3900

gucgacgagc cgaagcaccu cgacuacgug gacaagcaca aggacgaguu caaggagcug     3960

cuggacgugg ucagcaacuu cuccaagaag uacacccucg ccgaggggaa ccuggagaag     4020

aucaaggagc uguacgccca gaacaacggc gaggaccuca aggagcuggc cagcuccuuc     4080

aucaaccugc ucacguucac cgccaucggc gcgcccgcca ccuucaaguu cuucgacaag     4140

aacaucgacc gcaagcggua caccagcacg accgagaucc ugaacgccac ccugauccac     4200

caguccauca ccgggcucua cgagacgcgc aucgaccuga acaagcuggg cggggacaag     4260

cggcccgccg ccaccaagaa ggcgggccag gccaagaaga agaaguaagg acuagugaag     4320

ggaacaccca aauuuaauuc agccuuaagc acaauuaauu aagagugaaa cguaauugua     4380

caagcaguug gucacccacc auagggcaug aucaacaccg caaccuuucc uuuuuccccc     4440

agugauucug aaaaaccccu cuucccuuca gcuugcuuag auguuccaaa uuuaguaagc     4500

uuaaggcggc cuacagaaga aaaagaaaaa aaaggccaca aaaguucccu cucacuuuca     4560

guaaauaaaa uaaaagcagc aacagaaaua aagaaauaaa ugaaauucaa aaugaaauaa     4620

auauuguguu gugcagcauu aaaaaaucaa uaaaaauuaa aaaugagcaa gaucuaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag     4740

aauu                                                                  4744


<210>  2355
<211>  3877
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2355
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgcagac caccaaccug      240

agcuacaucc ucggccugga ccuggggauc gccuccgugg gcugggccgu ggucgagauc      300

aacgagaacg aggaccccau cgggcucauc gacgugggcg ugcggaucuu cgagcgcgcc      360

gaggucccca agacgggcga gagccuggcc cugucccggc gccucgcgcg gagcacccgc      420

cggcugaucc gccggcgcgc ccaccggcug cuccuggcca agcgcuuccu gaagcgggag      480

gggauccucu ccaccaucga ccuggagaag ggccugccca accaggccug ggagcuccgc      540

guggcggggc uggagcggcg ccugagcgcc aucgaguggg gcgccgugcu ccugcaccug      600

aucaagcacc ggggguaccu cuccaagcgc aagaacgaga gccagaccaa caacaaggag      660

cugggcgccc ugcucuccgg cgucgcccag aaccaccagc ugcugcagag cgacgacuac      720

cggacgccgg cggagcucgc ccugaagaag uucgccaagg aggaggggca cauccgcaac      780

cagcggggcg ccuacaccca caccuucaac cgccuggacc uccuggccga gcugaaccuc      840

cuguucgcgc agcagcacca guucgggaac ccccacugca aggagcacau ccagcaguac      900

augaccgagc ugcucaugug gcagaagccc gcccuguccg gcgaggccau ccugaagaug      960

cucggcaagu gcacgcacga gaagaacgag uucaaggccg ccaagcacac cuacagcgcg     1020

gagcgcuucg uguggcugac caagcugaac aaccuccgga uccuggagga cggggccgag     1080

cgcgcccuga acgaggagga gcggcagcuc cugaucaacc accccuacga gaaguccaag     1140

cugaccuacg cccaggugcg caagcuccug ggccugagcg agcaggccau cuucaagcac     1200

cuccgguacu ccaaggagaa cgcggagagc gccacguuca uggagcucaa ggccuggcac     1260

gccauccgca aggcgcugga gaaccagggg cugaaggaca ccuggcagga ccucgccaag     1320

aagccggacc ugcuggacga gaucggcacc gccuucuccc ucuacaagac cgacgaggac     1380

auccagcagu accugacgaa caaggucccc aacagcguga ucaacgcccu gcucgugucc     1440

cugaacuucg acaaguucau cgagcugagc cucaagagcc ugcggaagau ccugccccuc     1500

auggagcagg ggaagcgcua cgaccaggcc ugccgggaga ucuacggcca ccacuacggc     1560

gaggcgaacc agaagaccuc ccagcugcug cccgccaucc cggcccagga gauccgcaac     1620

cccgucgugc uccggacccu gagccaggcc cgcaagguga ucaacgccau cauccggcag     1680

uacggguccc ccgcgcgcgu ccacaucgag accggccggg agcugggcaa gagcuucaag     1740

gagcgccggg agauccagaa gcagcaggag gacaaccgca cgaagcggga guccgccgug     1800

cagaaguuca aggagcuguu cagcgacuuc uccagcgagc ccaaguccaa ggacauccug     1860

aaguuccgcc uguacgagca gcagcacggg aagugccucu acagcgggaa ggagaucaac     1920

auccaccgcc ugaacgagaa gggcuacgug gagaucgacc acgcccugcc guucucccgg     1980

accugggacg acagcuucaa caacaagguc cucgugcugg ccuccgagaa ccagaacaag     2040

ggcaaccaga cccccuacga guggcugcag gggaagauca acagcgagcg cuggaagaac     2100

uucguggccc ucguccuggg cucccagugc agcgcggcca agaagcagcg gcugcucacc     2160

caggugaucg acgacaacaa guucaucgac cgcaaccuga acgacacgcg guacaucgcc     2220

cgcuuccugu ccaacuacau ccaggagaac cuccugcugg ucgggaagaa caagaagaac     2280

guguucaccc ccaacggcca gaucaccgcc cuccugcgga gccgcugggg gcugaucaag     2340

gcgcgggaga acaacaaccg ccaccacgcc cucgacgcca ucguggucgc cugcgccacc     2400

cccuccaugc agcagaagau cacgcgguuc auccgcuuca aggaggugca cccguacaag     2460

aucgagaacc gguacgagau gguggaccag gagagcggcg agaucaucag cccccacuuc     2520

cccgagcccu gggcguacuu ccgccaggag gucaacaucc ggguguucga caaccacccg     2580

gacaccgugc ugaaggagau gcugcccgac cgcccccagg ccaaccacca guucguccag     2640

ccccucuucg ugucccgggc cccgacccgc aagaugagcg gccaggggca cauggagacc     2700

aucaaguccg ccaagcggcu ggccgagggc aucagcgugc ugcgcauccc ccucacgcag     2760

cugaagccca accugcucga gaacaugguc aacaaggagc gggagccggc gcuguacgcc     2820

gggcugaagg cccgccucgc cgaguucaac caggaccccg ccaaggcguu cgccaccccc     2880

uucuacaagc agggcggcca gcaggucaag gccauccgcg uggagcaggu ccagaagucc     2940

ggggugcugg ugcgggagaa caacggcgug gccgacaacg cgagcaucgu gcgcaccgac     3000

guguucauca agaacaacaa guucuuccug guccccaucu acaccuggca ggucgccaag     3060

gggauccucc cgaacaaggc caucguggcc cacaagaacg aggacgagug ggaggagaug     3120

gacgagggcg ccaaguucaa guucucccug uuccccaacg accuggugga gcucaagacg     3180

aagaaggagu acuucuucgg guacuacauc ggccuggacc gggcgaccgg caacaucagc     3240

cugaaggagc acgacgggga gaucuccaag ggcaaggacg ggguguaccg cgugggcguc     3300

aagcucgccc ugagcuucga gaaguaccag guggacgagc uggggaagaa ccggcagauc     3360

ugccgccccc agcagcggca gcccgugcgc aagcggccgg ccgccaccaa gaaggccggc     3420

caggcgaaga agaagaagua aggacuagug aagggaacac ccaaauuuaa uucagccuua     3480

agcacaauua auuaagagug aaacguaauu guacaagcag uuggucaccc accauagggc     3540

augaucaaca ccgcaaccuu uccuuuuucc cccagugauu cugaaaaacc ccucuucccu     3600

ucagcuugcu uagauguucc aaauuuagua agcuuaaggc ggccuacaga agaaaaagaa     3660

aaaaaaggcc acaaaaguuc ccucucacuu ucaguaaaua aaauaaaagc agcaacagaa     3720

auaaagaaau aaaugaaauu caaaaugaaa uaaauauugu guugugcagc auuaaaaaau     3780

caauaaaaau uaaaaaugag caagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                              3877


<210>  2356
<211>  3955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2356
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggccgc cuucaagccc      240

aaccccauca acuacauccu gggccucgac aucgggaucg ccagcguggg cugggccaug      300

guggagaucg acgaggacga gaaccccauc ugccugaucg accugggggu ccggguguuc      360

gagcgcgcgg aggugccgaa gaccggcgac ucccucgcca uggcccggcg ccuggcccgg      420

agcguccgcc ggcugacccg ccggcgcgcg caccggcucc ugcgcgcccg gcgccugcuc      480

aagcgggagg gcgugcugca ggccgccgac uucgacgaga acgggcugau caagucccuc      540

cccaacacgc ccuggcagcu gcgcgccgcg gcccuggacc ggaagcucac gccccuggag      600

uggagcgccg ugcugcucca ccugaucaag caccgcggcu accuguccca gcggaagaac      660

gagggggaga ccgccgacaa ggagcucggc gcccugcuga agggggucgc ggacaacgcc      720

cacgcccucc agaccggcga cuuccgcacc ccggccgagc uggcccugaa caaguucgag      780

aaggagagcg gccacauccg gaaccagcgc ggggacuacu cccacaccuu cagccgcaag      840

gaccuccagg cggagcugau ccuccucuuc gagaagcaga aggaguucgg caacccccac      900

guguccgggg gccugaagga gggcaucgag acccugcuca ugacccagcg gcccgcccug      960

agcggggacg ccgugcagaa gaugcugggc cacugcacgu ucgagcccgc cgagccgaag     1020

gccgcgaaga acaccuacac cgccgagcgc uucaucuggc ucaccaagcu gaacaaccug     1080

cggauccucg agcagggguc cgagcgcccc cugacggaca ccgagcgggc cacccugaug     1140

gacgagcccu accgcaagag caagcucacc uacgcccagg cgcggaagcu gcugggccuc     1200

gaggacacgg ccuucuucaa ggggcugcgc uacggcaagg acaacgccga ggccuccacc     1260

cugauggaga ugaaggccua ccacgcgauc agccgggccc ucgagaagga gggccugaag     1320

gacaagaagu ccccgcugaa ccugagcccc gagcuccagg acgagaucgg gaccgccuuc     1380

ucccuguuca agaccgacga ggacaucacg ggccgccuga aggaccggau ccagcccgag     1440

auccucgagg cccugcugaa gcacaucagc uucgacaagu ucguccagau cucccucaag     1500

gcccugcgcc ggaucgugcc ccugauggag caggggaagc gcuacgacga ggcgugcgcc     1560

gagaucuacg gcgaccacua cgggaagaag aacaccgagg agaagaucua ccugcccccg     1620

auccccgccg acgagauccg gaaccccgug guccugcgcg cccugagcca ggcccggaag     1680

gugaucaacg gcgugguccg ccgguacggc agccccgcgc gcauccacau cgagaccgcc     1740

cgcgaggugg ggaaguccuu caaggaccgg aaggagaucg agaagcgcca ggaggagaac     1800

cggaaggacc gcgagaaggc cgccgcgaag uuccgggagu acuucccgaa cuucgugggc     1860

gagcccaaga gcaaggacau ccucaagcug cgccuguacg agcagcagca cgggaagugc     1920

cucuacuccg gcaaggagau caaccugggg cggcugaacg agaagggcua cgucgagauc     1980

gaccacgccc uccccuucag ccgcaccugg gacgacuccu ucaacaacaa ggugcuggug     2040

cugggcagcg agaaccagaa caaggggaac cagacgcccu acgaguacuu caacggcaag     2100

gacaacuccc gggaguggca ggaguucaag gcccgcgucg agaccagccg guucccgcgc     2160

uccaagaagc agcggauccu ccugcagaag uucgacgagg acggguucaa ggagcgcaac     2220

cugaacgaca cccgguacgu gaaccgcuuc cucugccagu ucgucgccga ccggaugcgc     2280

cugaccggca agggcaagaa gcggguguuc gccagcaacg ggcagaucac gaaccugcuc     2340

cgcggcuucu gggggcugcg gaaggugcgc gcggagaacg accgccacca cgcccuggac     2400

gccgucgugg uggccugcuc caccgucgcc augcagcaga agaucacccg guucgugcgc     2460

uacaaggaga ugaacgcguu cgacggcaag accaucgaca aggagacggg ggaggugcuc     2520

caccagaaga cccacuuccc ccagcccugg gaguucuucg cccaggaggu caugauccgg     2580

guguucggca agccggacgg caagcccgag uucgaggagg ccgacacccc cgagaagcug     2640

cgcacccugc ucgccgagaa gcugagcucc cggcccgagg ccgugcacga guacgucacg     2700

ccgcuguucg ugagccgcgc gcccaaccgg aagauguccg ggcagggcca cauggagacc     2760

gucaagagcg ccaagcgccu cgacgagggg guguccgugc ugcgggugcc ccugacccag     2820

cucaagcuga aggaccugga gaagaugguc aaccgcgagc gggagcccaa gcucuacgag     2880

gcccugaagg cccgccugga ggcgcacaag gacgacccgg ccaaggccuu cgccgagccc     2940

uucuacaagu acgacaaggc cggcaaccgg acccagcagg ucaaggcggu gcgcguggag     3000

cagguccaga agaccggggu gugggugcgg aaccacaacg gcaucgccga caacgccacg     3060

auggugcgcg uggacgucuu cgagaagggc gacaaguacu accucgugcc caucuacagc     3120

uggcaggucg ccaaggggau ccugcccgac cgggccgugg ugcagggcaa ggacgaggag     3180

gacuggcagc ugaucgacga cuccuucaac uucaaguuca gccuccaccc gaacgaccug     3240

guggagguca ucaccaagaa ggcgcgcaug uucggguacu ucgccagcug ccaccggggc     3300

accgggaaca ucaacauccg cauccacgac cuggaccaca agaucggcaa gaacggcauc     3360

cucgagggga ucggcgugaa gaccgcccug uccuuccaga aguaccagau cgacgagcug     3420

gggaaggaga uccgccccug ccggcucaag aagcgcccgc ccgugcggaa gcgccccgcc     3480

gccacgaaga aggcgggcca ggccaagaag aagaaguaag gacuagugaa gggaacaccc     3540

aaauuuaauu cagccuuaag cacaauuaau uaagagugaa acguaauugu acaagcaguu     3600

ggucacccac cauagggcau gaucaacacc gcaaccuuuc cuuuuucccc cagugauucu     3660

gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa auuuaguaag cuuaaggcgg     3720

ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc ucucacuuuc aguaaauaaa     3780

auaaaagcag caacagaaau aaagaaauaa augaaauuca aaaugaaaua aauauugugu     3840

ugugcagcau uaaaaaauca auaaaaauua aaaaugagca agaucuaaaa aaaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu          3955


<210>  2357
<211>  3961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2357
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagua ccacgugggc      240

aucgacgugg ggaccuucag cgucggccug gccgccaucg agguggacga cgccgggaug      300

cccaucaaga cccugucccu ggugagccac auccacgacu ccggccugga ccccgacgag      360

aucaagagcg ccgucacgcg gcucgcgucc agcggcaucg cccgccggac ccgccggcug      420

uaccgccgga agcgccggcg ccugcagcag cucgacaagu ucauccagcg gcaggggugg      480

cccgugaucg agcuggagga cuacuccgac ccgcuguacc ccuggaaggu gcgcgccgag      540

cucgccgcga gcuacaucgc cgacgagaag gagcggggcg agaagcuguc cgucgcccug      600

cgccacaucg cccggcaccg cggguggcgg aaccccuacg ccaaggugag cucccucuac      660

cugcccgacg gcccgagcga cgcguucaag gccauccgcg aggagaucaa gcgggccucc      720

gggcagcccg ugcccgagac cgccaccguc ggccagaugg ugacgcugug cgagcucggc      780

acccugaagc ugcgcgggga gggcggggug cucagcgccc ggcugcagca guccgacuac      840

gcgcgcgaga uccaggagau cugccgcaug caggagaucg gccaggagcu guaccggaag      900

aucaucgacg ucguguucgc cgccgagagc cccaagggcu ccgccagcuc ccgcgugggg      960

aaggacccgc uccagcccgg caagaaccgg gcccugaagg cgagcgacgc cuuccagcgc     1020

uaccggaucg ccgcccugau cgggaaccuc cgcguccggg uggacggcga gaagcgcauc     1080

cugagcgugg aggagaagaa ccuggucuuc gaccaccucg ugaaccugac ccccaagaag     1140

gagcccgagu gggugaccau cgccgagauc cuggggaucg accggggcca gcucaucggc     1200

acggcgacca ugaccgacga cggggagcgc gccggcgccc ggccgcccac ccacgacacg     1260

aaccgcucca ucgucaacag ccggaucgcg ccccuggugg acugguggaa gaccgccucc     1320

gcccuggagc agcacgccau ggucaaggcc cucagcaacg ccgaggugga cgacuucgac     1380

ucccccgagg gggcgaaggu gcaggccuuc uucgccgacc uggacgacga cguccacgcc     1440

aagcuggaca gccuccaccu cccggugggc cgcgccgcgu acuccgagga cacccuggug     1500

cggcucaccc gccggaugcu gagcgacggg gucgaccugu acacggcccg ccuccaggag     1560

uucggcaucg agcccuccug gaccccgccc acgccccgga ucggcgagcc cguggggaac     1620

cccgccgugg accgcguccu gaagaccgug agccgcuggc uggaguccgc caccaagacc     1680

uggggcgccc ccgagcgggu gaucaucgag cacguccgcg agggguucgu gaccgagaag     1740

cgggcgcgcg agauggacgg cgacaugcgg cgccgggccg cccgcaacgc caagcuguuc     1800

caggagaugc aggagaagcu gaacgugcag gggaagccga gccgggcgga ccuguggcgc     1860

uaccaguccg uccagcggca gaacugccag ugcgccuacu gcggcagccc caucaccuuc     1920

uccaacagcg agauggacca caucgugccc cgcgccggcc agggguccac gaacacccgg     1980

gagaaccucg uggccgucug ccaccgcugc aaccagagca agggcaacac cccguucgcc     2040

aucugggcga agaacaccuc caucgagggg gugagcguga aggaggccgu cgagcggacg     2100

cgccacuggg ugaccgacac cggcaugcgg agcaccgacu ucaagaaguu cacgaaggcc     2160

guggucgagc gcuuccagcg ggccaccaug gacgaggaga ucgacgcccg cuccauggag     2220

agcguggcgu ggauggccaa cgagcugcgg ucccgcgugg cccagcacuu cgccagccac     2280

ggcaccaccg uccgcgugua ccgggggucc cugacggccg aggcgcgccg ggccagcggc     2340

aucuccggga agcugaaguu cuucgacggc gugggcaaga gccgccucga ccggcgccac     2400

cacgccaucg acgccgcggu caucgccuuc accuccgacu acguggccga gacccuggcc     2460

gugcggagca accugaagca gucccaggcc caccgccagg aggcgcccca guggcgggag     2520

uucaccggga aggacgccga gcaccgcgcc gccuggcggg ucuggugcca gaagauggag     2580

aagcucagcg cccugcugac cgaggaccuc cgcgacgacc gggugguggu cauguccaac     2640

gugcgccugc ggcugggcaa cgggagcgcg cacaaggaga cgaucggcaa gcucuccaag     2700

gugaagcuga gcucccagcu gagcgucagc gacaucgaca aggccuccag cgaggcccuc     2760

uggugcgccc ugacccgcga gcccggguuc gaccccaagg agggccugcc ggccaacccc     2820

gagcggcaca uccgcgugaa cgggacccac guguacgcgg gcgacaacau cggccucuuc     2880

cccgucuccg ccgggagcau cgcccugcgg ggcggguacg ccgagcuggg cuccagcuuc     2940

caccacgccc gcguguacaa gaucaccucc gggaagaagc ccgcguucgc caugcuccgc     3000

guguacacga ucgaccugcu gccguaccgg aaccaggacc ucuucagcgu cgagcugaag     3060

ccccagacca uguccaugcg ccaggccgag aagaagcugc gggacgcccu cgcgaccggc     3120

aacgccgagu accugggcug gcugguggug gacgacgagc ucgucgugga caccagcaag     3180

aucgccacgg accaggugaa ggccgucgag gccgagcugg ggaccauccg ccgguggcgc     3240

guggacggcu ucuucucccc cagcaagcug cggcuccgcc cccugcagau guccaaggag     3300

gggaucaaga aggagagcgc gccggagcug uccaagauca ucgaccggcc cggcuggcuc     3360

cccgccguca acaagcuguu cagcgacggg aacgugaccg ugguccgccg ggacucccug     3420

ggccgcgugc ggcucgagag caccgcccac cuccccguga cguggaaggu ccagaagcgc     3480

ccggccgcca ccaagaaggc gggccaggcc aagaagaaga aguaaggacu agugaaggga     3540

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa     3600

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu     3660

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua     3720

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua     3780

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     3840

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau     3960

u                                                                     3961


<210>  2358
<211>  3661
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2358
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggcccg gauccuggcc      240

uucgacaucg gcaucagcuc caucgggugg gccuucagcg agaacgacga gcucaaggac      300

ugcggcgugc gcaucuucac caagguggag aaccccaaga ccggggaguc ccuggcccug      360

ccccggcgcc ucgcgcggag cgcccgcaag cggcuggccc gccggaaggc ccgccugaac      420

caccucaagc accugaucgc gaacgaguuc aagcugaacu acgaggacua ccaguccuuc      480

gacgagagcc ucgccaaggc cuacaagggc ucccugauca gccccuacga gcugcgguuc      540

cgcgcccuca acgagcugcu guccaagcag gacuucgccc gggucauccu ccacaucgcg      600

aagcgccggg gcuacgacga caucaagaac agcgacgaca aggagaaggg ggccauccug      660

aaggccauca agcagaacga ggagaagcug gccaacuacc aguccguggg cgaguaccuc      720

uacaaggagu acuuccagaa guucaaggag aacagcaagg aguucacgaa cgugcgcaac      780

aagaaggagu ccuacgagcg gugcaucgcc cagagcuucc ugaaggacga gcugaagcuc      840

aucuucaaga agcagcgcga guucggguuc uccuucagca agaaguucga ggaggagguc      900

cuguccgugg cguucuacaa gcgggcccug aaggacuuca gccaccucgu gggcaacugc      960

uccuucuuca ccgacgagaa gcgcgccccg aagaacagcc cccuggccuu cauguucguc     1020

gcccugaccc ggaucaucaa ccuccugaac aaccugaaga acaccgaggg gauccucuac     1080

acgaaggacg accugaacgc gcugcucaac gaggugcuga agaacggcac ccugaccuac     1140

aagcagacca agaagcuccu gggccugagc gacgacuacg aguucaaggg ggagaagggc     1200

acguacuuca ucgaguucaa gaaguacaag gaguucauca aggcccucgg ggagcacaac     1260

cugucccagg acgaccugaa cgagaucgcc aaggacauca cccucaucaa ggacgagauc     1320

aagcugaaga aggcccuggc caaguacgac cucaaccaga accagaucga cagccugucc     1380

aagcuggagu ucaaggacca ccucaacauc agcuucaagg cgcugaagcu ggugacgccc     1440

cugaugcucg agggcaagaa guacgacgag gccugcaacg agcugaaccu gaaggucgcc     1500

aucaacgagg acaagaagga cuuccucccc gccuucaacg agaccuacua caaggacgag     1560

gugaccaacc cggugguccu gcgcgcgauc aaggaguacc gcaaggugcu gaacgcccuc     1620

cugaagaagu acggcaaggu gcacaagauc aacaucgagc uggcccggga ggucgggaag     1680

aaccacuccc agcgcgccaa gaucgagaag gagcagaacg agaacuacaa ggccaagaag     1740

gacgcggagc ucgagugcga gaagcugggc cugaagauca acagcaagaa cauccucaag     1800

cugcggcugu ucaaggagca gaaggaguuc ugcgccuacu ccggggagaa gaucaagauc     1860

agcgaccucc aggacgagaa gaugcuggag aucgaccaca ucuaccccua cucccgcagc     1920

uucgacgacu ccuacaugaa caaggugcug guguucacca agcagaacca ggagaagcuc     1980

aaccagaccc ccuucgaggc cuucggcaac gacagcgcca aguggcagaa gaucgagguc     2040

cuggccaaga accugcccac caagaagcag aagcggaucc ucgacaagaa cuacaaggac     2100

aaggagcaga agaacuucaa ggaccgcaac cugaacgaca cgcgguacau cgcgcgccug     2160

gugcucaacu acaccaagga cuaccuggac uuccugccgc ucuccgacga cgagaacacc     2220

aagcugaacg acacccagaa ggggagcaag gugcacgucg aggccaaguc cggcaugcug     2280

acgagcgccc uccggcacac cuggggcuuc uccgccaagg accgcaacaa ccaccuccac     2340

cacgccaucg acgcggugau caucgccuac gccaacaaca gcaucgucaa ggccuucucc     2400

gacuucaaga aggagcagga gagcaacagc gcggagcugu acgccaagaa gaucuccgag     2460

cuggacuaca agaacaagcg gaaguucuuc gagcccuuca gcggguuccg ccagaaggug     2520

cuggacaaga ucgacgagau cuucgugucc aagcccgagc ggaagaagcc cagcggcgcc     2580

cugcacgagg agaccuuccg caaggaggag gaguucuacc aguccuacgg gggcaaggag     2640

gggguccuca aggcccugga gcugggcaag auccggaagg ugaacggcaa gaucgugaag     2700

aacggggaca uguuccgcgu cgacaucuuc aagcacaaga agaccaacaa guucuacgcc     2760

gugccgaucu acacgaugga cuucgcgcuc aaggugcugc ccaacaaggc cgucgcccgg     2820

agcaagaagg gcgagaucaa ggacuggauc cugauggacg agaacuacga guucugcuuc     2880

ucccucuaca aggacagccu gauccugauc cagaccaagg acaugcagga gcccgaguuc     2940

guguacuaca acgccuucac cuccagcacc gugucccuca ucgucagcaa gcacgacaac     3000

aaguucgaga cgcuguccaa gaaccagaag auccuguuca agaacgccaa cgagaaggag     3060

gugaucgcca agagcaucgg gauccagaac cucaaggugu ucgagaagua caucgucucc     3120

gcgcugggcg aggugaccaa ggccgaguuc cgccagcggg aggacuucaa gaagaagcgc     3180

cccgccgcca ccaagaaggc ggggcaggcc aagaagaaga aguaaggacu agugaaggga     3240

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa     3300

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu     3360

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua     3420

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua     3480

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     3540

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau     3660

u                                                                     3661


<210>  2359
<211>  4072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2359
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgagcga ccuggugcuc      240

ggccuggaca ucgggaucgg cuccgugggg gucggcaucc ugaacaaggu gaccggcgag      300

aucauccaca agaacagccg gaucuucccc gccgcccagg ccgagaacaa ccucgugcgc      360

cggaccaacc gccaggggcg gcgccuggcc cggcgcaaga agcaccggcg cguccggcug      420

aaccgccucu ucgaggaguc cggccugauc acggacuuca ccaagaucag caucaaccug      480

aaccccuacc agcuccgggu gaaggggcug accgacgagc uguccaacga ggagcuguuc      540

aucgcgcuga agaacauggu gaagcaccgc ggcaucagcu accuggacga cgccuccgac      600

gacgggaaca gcuccgucgg cgacuacgcc cagaucguga aggagaacag caagcagcuc      660

gagaccaaga cgcccggcca gauccagcug gagcgguacc agaccuacgg gcagcugcgc      720

ggcgacuuca ccguggagaa ggacgggaag aagcaccggc ucaucaacgu cuucccgacc      780

uccgccuacc gcagcgaggc gcugcggauc cugcagacgc agcaggaguu caacccccag      840

aucaccgacg aguucaucaa ccgcuaccuc gagauccuga ccggcaagcg gaaguacuac      900

cacggccccg ggaacgagaa gucccgcacc gacuacggcc gcuaccggac gagcggggag      960

acccuggaca acaucuucgg cauccucauc gggaagugca ccuucuaccc cgacgaguuc     1020

cgcgccgcca aggccuccua caccgcccag gaguucaacc ugcugaacga ccucaacaac     1080

cugacggugc cgaccgagac caagaagcug agcaaggagc agaagaacca gaucaucaac     1140

uacgugaaga acgagaaggc gaugggcccc gccaagcugu ucaaguacau cgccaagcuc     1200

cuguccugcg acgucgccga caucaagggc uaccggaucg acaagagcgg gaaggccgag     1260

auccacaccu ucgaggcgua ccgcaagaug aagacgcucg agacccugga caucgagcag     1320

auggaccggg agacccugga caagcucgcc uacgugcuga cccugaacac ggagcgcgag     1380

ggcauccagg aggcccucga gcacgaguuc gccgacgggu ccuucagcca gaagcagguc     1440

gacgagcugg ugcaguuccg gaaggccaac agcuccaucu ucggcaaggg guggcacaac     1500

uucagcguga agcugaugau ggagcucauc cccgagcugu acgagaccuc cgaggagcag     1560

augaccaucc ugacccgccu cggcaagcag aagacgacca gcuccagcaa caagaccaag     1620

uacaucgacg agaagcugcu gaccgaggag aucuacaacc ccguggucgc gaaguccgug     1680

cggcaggcca ucaagaucgu gaacgccgcc aucaaggagu acggcgacuu cgacaacauc     1740

gucaucgaga uggcccgcga gacgaacgag gacgacgaga agaaggcgau ccagaagauc     1800

cagaaggcca acaaggacga gaaggacgcc gccaugcuca aggcggccaa ccaguacaac     1860

gggaaggccg agcugccgca cagcguguuc cacggccaca agcagcuggc caccaagauc     1920

cggcuguggc accagcaggg ggagcgcugc cucuacaccg gcaagaccau cuccauccac     1980

gaccugauca acaacagcaa ccaguucgag gucgaccaca uccugccccu cuccaucacg     2040

uucgacgaca gccuggccaa caaggugcug guguacgcga ccgccaacca ggagaagggg     2100

cagcggaccc ccuaccaggc ccucgacucc auggacgacg ccuggagcuu ccgcgagcug     2160

aaggccuucg uccgggaguc caagacccug agcaacaaga agaaggagua ccuccugacc     2220

gaggaggaca ucuccaaguu cgacgugcgc aagaaguuca ucgagcggaa ccugguggac     2280

acgcgcuacg cgagccgggu cgugcucaac gcccugcagg agcacuuccg cgcccacaag     2340

aucgacacca agguguccgu cgugcgcggc caguucacca gccagcugcg gcgccacugg     2400

ggcaucgaga agacccggga cacguaccac caccacgccg uggacgcccu caucaucgcg     2460

gccagcuccc agcugaaccu guggaagaag cagaagaaca cccucgucag cuacuccgag     2520

gaccagcugc uggacaucga gaccggggag cucaucagcg acgacgagua caaggagucc     2580

guguucaagg cccccuacca gcacuucgug gacacccuca agagcaagga guucgaggac     2640

uccauccugu ucagcuacca ggucgacucc aaguucaacc gcaagaucag cgacgccacg     2700

aucuacgcga cccggcaggc caaggugggc aaggacaagg ccgacgagac cuacgugcuc     2760

gggaagauca aggacaucua cacccaggac ggcuacgacg ccuucaugaa gaucuacaag     2820

aaggacaagu ccaaguuccu gauguaccgc cacgacccgc agacguucga gaaggucauc     2880

gagcccaucc uggagaacua ccccaacaag cagaucaacg agaagggcaa ggaggugccc     2940

ugcaacccgu uccucaagua caaggaggag cacggguaca uccggaagua cagcaagaag     3000

ggcaacgggc ccgagaucaa gucccugaag uacuacgaca gcaagcuggg caaccacauc     3060

gacaucaccc ccaaggacuc caacaacaag gugguccucc agagcguguc ccccuggcgc     3120

gccgacgugu acuucaacaa gaccaccggg aaguacgaga uccugggccu gaaguacgcg     3180

gaccuccagu ucgagaaggg cacggggacc uacaagauca gccaggagaa guacaacgac     3240

aucaagaaga aggagggcgu cgacagcgac uccgaguuca aguucacccu guacaagaac     3300

gaccugcucc uggugaagga caccgagacg aaggagcagc agcuguuccg guuccucagc     3360

cgcaccaugc cgaagcagaa gcacuacgug gagcugaagc ccuacgacaa gcagaaguuc     3420

gagggcgggg aggcccugau caagguccuc gggaacgugg ccaacuccgg ccagugcaag     3480

aagggccugg gcaagagcaa caucuccauc uacaaggugc ggaccgacgu ccuggggaac     3540

cagcacauca ucaagaacga gggggacaag cccaagcucg acuucaagcg ccccgccgcc     3600

accaagaagg cgggccaggc caagaagaag aaguaaggac uagugaaggg aacacccaaa     3660

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu     3720

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa     3780

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu     3840

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     3900

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     3960

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     4020

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             4072


<210>  2360
<211>  4630
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2360
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaccca guucgagggc      240

uucaccaacc uguaccaggu gagcaagacg cuccgguucg agcugauccc ccaggggaag      300

acccugaagc acauccagga gcagggcuuc aucgaggagg acaaggcccg caacgaccac      360

uacaaggagc ucaagcccau caucgaccgg aucuacaaga ccuacgccga ccagugccug      420

cagcuggugc agcucgacug ggagaaccug uccgccgcca ucgacagcua ccgcaaggag      480

aagaccgagg agacgcggaa cgcgcugauc gaggagcagg ccaccuaccg caacgccauc      540

cacgacuacu ucaucgggcg gaccgacaac cucaccgacg ccaucaacaa gcgccacgcg      600

gagaucuaca agggccuguu caaggccgag cuguucaacg gcaagguccu caagcagcug      660

gggacgguga ccaccaccga gcacgagaac gcccugcucc gguccuucga caaguucacg      720

accuacuuca gcggcuucua cgagaaccgc aagaacgugu ucuccgccga ggacaucagc      780

accgccaucc cccaccggau cguccaggac aacuucccga aguucaagga gaacugccac      840

aucuucaccc gccugaucac ggcggugccc ucccugcggg agcacuucga gaacgugaag      900

aaggccaucg ggaucuucgu cagcaccucc aucgaggagg uguucagcuu ccccuucuac      960

aaccagcucc ugacccagac ccagaucgac cuguacaacc agcuccuggg cgggaucucc     1020

cgcgaggccg gcacggagaa gaucaagggc cugaacgagg ugcucaaccu ggccauccag     1080

aagaacgacg agaccgccca caucaucgcg agccugcccc accgguucau cccccucuuc     1140

aagcagaucc uguccgaccg caacacccug agcuucaucc ucgaggaguu caaguccgac     1200

gaggagguca uccagagcuu cugcaaguac aagacccugc ugcggaacga gaacgugcuc     1260

gagacggccg aggcccuguu caacgagcug aacuccaucg accucaccca caucuucauc     1320

agccacaaga agcuggagac caucuccagc gcccugugcg accacuggga cacccuccgc     1380

aacgcccugu acgagcggcg caucagcgag cugacgggga agaucaccaa guccgcgaag     1440

gagaaggugc agcggagccu caagcacgag gacaucaacc ugcaggagau caucuccgcc     1500

gccggcaagg agcugagcga ggccuucaag cagaagaccu ccgagauccu cagccacgcc     1560

cacgcggccc uggaccagcc gcugcccacc acgcucaaga agcaggagga gaaggagauc     1620

cugaaguccc agcuggacag ccugcucggg cuguaccacc uccucgacug guucgccguc     1680

gacgagucca acgaggugga ccccgaguuc agcgcccgcc ugaccggcau caagcuggag     1740

auggagccgu cccucagcuu cuacaacaag gcgcgcaacu acgccaccaa gaagcccuac     1800

uccguggaga aguucaagcu gaacuuccag augcccaccc uggccagcgg cugggacguc     1860

aacaaggaga agaacaacgg ggccauccuc uucgugaaga acggccugua cuaccugggg     1920

aucaugccca agcagaaggg ccgguacaag gcccucuccu ucgagccgac ggagaagacc     1980

agcgaggggu ucgacaagau guacuacgac uacuuccccg acgcggccaa gaugaucccc     2040

aagugcucca cccagcugaa ggccgugacc gcccacuucc agacccacac gacccccauc     2100

cugcucagca acaacuucau cgagccgcug gagaucacca aggagaucua cgaccugaac     2160

aaccccgaga aggagcccaa gaaguuccag accgccuacg cgaagaagac gggcgaccag     2220

aagggcuacc gcgaggcccu cugcaagugg aucgacuuca cccgggacuu ccuguccaag     2280

uacaccaaga ccacgagcau cgaccugagc ucccuccgcc ccagcuccca guacaaggac     2340

cugggggagu acuacgccga gcugaacccg cuccuguacc acaucagcuu ccagcggauc     2400

gccgagaagg agaucaugga cgccgucgag accggcaagc uguaccucuu ccagaucuac     2460

aacaaggacu ucgcgaaggg gcaccacggc aagcccaacc ugcacacccu guacuggacc     2520

ggccucuucu cccccgagaa ccuggccaag acgagcauca agcugaacgg gcaggccgag     2580

cuguucuacc gccccaaguc ccggaugaag cgcauggccc accggcuggg cgagaagaug     2640

cugaacaaga agcucaagga ccagaagacc ccgauccccg acacccugua ccaggagcug     2700

uacgacuacg ugaaccaccg ccucagccac gaccuguccg acgaggcgcg ggcccugcuc     2760

cccaacguga ucaccaagga ggucagccac gagaucauca aggaccgccg guucacgucc     2820

gacaaguucu ucuuccacgu gccgaucacc cugaacuacc aggccgccaa cagccccucc     2880

aaguucaacc agcgcgucaa cgccuaccug aaggagcacc ccgagacccc caucaucggg     2940

aucgaccggg gcgagcgcaa ccucaucuac aucaccguga ucgacagcac ggggaagauc     3000

cuggagcagc ggucccugaa caccauccag caguucgacu accagaagaa gcucgacaac     3060

cgcgagaagg agcggguggc ggcccgccag gccuggagcg ucgugggcac caucaaggac     3120

cugaagcagg gguaccuguc ccaggugauc cacgagaucg ucgaccucau gauccacuac     3180

caggccgugg ugguccugga gaaccugaac uucggcuuca agagcaagcg caccggcauc     3240

gccgagaagg cgguguacca gcaguucgag aagaugcuca ucgacaagcu gaacugccug     3300

gugcucaagg acuacccggc cgagaagguc gggggcgugc ugaaccccua ccagcugacg     3360

gaccaguuca ccagcuucgc caagaugggg acccaguccg gcuuccucuu cuacgugccc     3420

gcccccuaca ccagcaagau cgacccgcug acgggcuucg ucgaccccuu cguguggaag     3480

accaucaaga accacgaguc ccggaagcac uuccuggagg gguucgacuu ccuccacuac     3540

gacgugaaga ccggcgacuu cauccugcac uucaagauga accgcaaccu gagcuuccag     3600

cgggggcucc ccggcuucau gcccgccugg gacaucgucu ucgagaagaa cgagacccag     3660

uucgacgcga aggggacgcc guucaucgcc ggcaagcgca ucgugcccgu gaucgagaac     3720

caccgguuca ccggccgcua ccgggaccug uaccccgcca acgagcugau cgcccuccug     3780

gaggagaagg ggaucgucuu ccgcgacggc uccaacaucc ugcccaagcu ccuggagaac     3840

gacgacagcc acgcgaucga caccauggug gcccugaucc gguccgugcu ccagaugcgc     3900

aacagcaacg ccgccaccgg ggaggacuac aucaacuccc cgguccggga ccugaacggc     3960

gugugcuucg acagccgcuu ccagaacccc gaguggccca uggacgccga cgcgaacggg     4020

gccuaccaca ucgcccugaa gggccagcuc cugcugaacc accucaagga guccaaggac     4080

cugaagcugc agaacggcau cagcaaccag gacuggcucg ccuacaucca ggagcugcgg     4140

aacaagcgcc ccgccgcgac gaagaaggcc gggcaggcca agaagaagaa guaaggacua     4200

gugaagggaa cacccaaauu uaauucagcc uuaagcacaa uuaauuaaga gugaaacgua     4260

auuguacaag caguugguca cccaccauag ggcaugauca acaccgcaac cuuuccuuuu     4320

ucccccagug auucugaaaa accccucuuc ccuucagcuu gcuuagaugu uccaaauuua     4380

guaagcuuaa ggcggccuac agaagaaaaa gaaaaaaaag gccacaaaag uucccucuca     4440

cuuucaguaa auaaaauaaa agcagcaaca gaaauaaaga aauaaaugaa auucaaaaug     4500

aaauaaauau uguguugugc agcauuaaaa aaucaauaaa aauuaaaaau gagcaagauc     4560

uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaagaauu                                                            4630


<210>  2361
<211>  4609
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2361
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgagcau cuaccaggag      240

uucgugaaca aguacucccu gagcaagacc cuccgguucg agcugauccc ccagggcaag      300

acccuggaga acaucaaggc ccgcgggcuc auccuggacg acgagaagcg ggccaaggac      360

uacaagaagg ccaagcagau caucgacaag uaccaccagu ucuucaucga ggagauccug      420

uccagcgugu gcaucuccga ggaccuccug cagaacuaca gcgacgucua cuucaagcug      480

aagaaguccg acgacgacaa ccuccagaag gacuucaaga gcgccaagga cacgaucaag      540

aagcagaucu ccgaguacau caaggacagc gagaaguuca agaaccuguu caaccagaac      600

cugaucgacg cgaagaaggg ccaggagucc gaccucaucc uguggcugaa gcagagcaag      660

gacaacggga ucgagcuguu caaggccaac uccgacauca ccgacaucga cgaggcccuc      720

gagaucauca agagcuucaa gggcuggacc accuacuuca agggcuucca cgagaaccgc      780

aagaacgugu acuccagcaa cgacaucccc acguccauca ucuaccggau cguggacgac      840

aaccugccca aguuccucga gaacaaggcc aaguacgaga gccugaagga caaggcgccg      900

gaggccauca acuacgagca gaucaagaag gaccuggccg aggagcucac cuucgacauc      960

gacuacaaga ccuccgaggu caaccagcgc guguucagcc uggacgaggu guucgagauc     1020

gccaacuuca acaacuaccu gaaccagagc gggaucacca aguucaacac gaucaucggc     1080

gggaaguucg ucaacggcga gaacaccaag cggaagggga ucaacgagua caucaaccuc     1140

uacucccagc agaucaacga caagacccug aagaaguaca agaugagcgu gcuguucaag     1200

cagauccucu ccgacaccga gagcaagucc uucgugaucg acaagcugga ggacgacagc     1260

gacgucguga cgaccaugca guccuucuac gagcagaucg ccgcguucaa gaccguggag     1320

gagaagucca ucaaggagac ccugucccuc cuguucgacg accugaaggc ccagaagcuc     1380

gaccugagca agaucuacuu caagaacgac aagucccuga cggaccucag ccagcagguc     1440

uucgacgacu acuccgugau cggcaccgcc gugcuggagu acaucaccca gcagaucgcc     1500

cccaagaacc uggacaaccc cagcaagaag gagcaggagc ucaucgccaa gaagaccgag     1560

aaggcgaagu accugucccu ggagacgauc aagcucgccc uggaggaguu caacaagcac     1620

cgcgacaucg acaagcagug ccgguucgag gagauccugg ccaacuucgc cgccaucccc     1680

augaucuucg acgagaucgc gcagaacaag gacaaccucg cccagaucag caucaaguac     1740

cagaaccagg gcaagaagga ccugcugcag gccuccgccg aggacgacgu caaggccauc     1800

aaggaccucc uggaccagac caacaaccug cugcacaagc ucaagaucuu ccacaucagc     1860

caguccgagg acaaggcgaa cauccuggac aaggacgagc acuucuaccu gguguucgag     1920

gagugcuacu ucgagcucgc caacaucgug ccgcuguaca acaagauccg caacuacauc     1980

acccagaagc ccuacagcga cgagaaguuc aagcugaacu ucgagaacag cacccucgcc     2040

aacggguggg acaagaacaa ggagcccgac aacacggcca uccuguucau caaggacgac     2100

aaguacuacc ugggcgucau gaacaagaag aacaacaaga ucuucgacga caaggcgauc     2160

aaggagaaca agggggaggg cuacaagaag aucguguaca agcuccugcc cggcgccaac     2220

aagaugcugc cgaagguguu cuucuccgcc aagagcauca aguucuacaa ccccuccgag     2280

gacauccucc ggauccgcaa ccacagcacc cacaccaaga acgggagccc ccagaagggc     2340

uacgagaagu ucgaguucaa caucgaggac ugccggaagu ucaucgacuu cuacaagcag     2400

agcaucucca agcaccccga guggaaggac uucggguucc gcuucagcga cacccagcgg     2460

uacaacucca ucgacgaguu cuaccgcgag gucgagaacc agggcuacaa gcugacguuc     2520

gagaacauca gcgaguccua caucgacagc guggucaacc aggggaagcu guaccucuuc     2580

cagaucuaca acaaggacuu cuccgccuac agcaagggcc ggccgaaccu gcacacccug     2640

uacuggaagg cccucuucga cgagcgcaac cugcaggacg ugguguacaa gcugaacggc     2700

gaggcggagc uguucuaccg gaagcagucc auccccaaga agaucaccca ccccgccaag     2760

gaggccaucg ccaacaagaa caaggacaac cccaagaagg agagcgucuu cgaguacgac     2820

cucaucaagg acaagcgcuu caccgaggac aaguucuucu uccacugccc gaucacgauc     2880

aacuucaagu ccagcggggc caacaaguuc aacgacgaga ucaaccugcu ccugaaggag     2940

aaggcgaacg acgugcacau ccugagcauc gaccggggcg agcgccaccu cgccuacuac     3000

acccuggugg acgggaaggg caacaucauc aagcaggaca ccuucaacau caucgggaac     3060

gaccgcauga agaccaacua ccacgacaag cuggccgcca ucgagaagga ccgggacucc     3120

gcccgcaagg acuggaagaa gaucaacaac aucaaggaga ugaaggaggg cuaccucagc     3180

caggucgugc acgagaucgc gaagcuggug aucgaguaca acgccaucgu cguguucgag     3240

gaccugaacu ucggcuucaa gcgggggcgc uucaaggugg agaagcaggu cuaccagaag     3300

cucgagaaga ugcugaucga gaagcugaac uaccucgugu ucaaggacaa cgaguucgac     3360

aagacgggcg gggugcugcg ggccuaccag cugaccgccc ccuucgagac cuucaagaag     3420

augggcaagc agaccgggau caucuacuac guccccgcgg gcuucaccuc caagaucugc     3480

cccgugacgg gcuucgugaa ccagcucuac ccgaaguacg agagcgucuc caagagccag     3540

gaguucuucu ccaaguucga caagaucugc uacaaccugg acaaggggua cuucgaguuc     3600

agcuucgacu acaagaacuu cggcgacaag gccgccaagg ggaaguggac caucgccucc     3660

uucggcagcc gccugaucaa cuuccggaac uccgacaaga accacaacug ggacacccgc     3720

gagguguacc ccaccaagga gcucgagaag cugcugaagg acuacagcau cgaguacggc     3780

cacggggagu gcaucaaggc cgcgaucugc ggcgaguccg acaagaaguu cuucgccaag     3840

cucacgagcg ugcugaacac cauccugcag augcggaacu ccaagaccgg gaccgagcuc     3900

gacuaccuga ucagccccgu cgccgacgug aacggcaacu ucuucgacuc ccgccaggcc     3960

cccaagaaca ugccgcagga cgccgacgcg aacggggccu accacaucgg ccugaagggc     4020

cucaugcugc uggggcggau caagaacaac caggagggca agaagcucaa ccuggugauc     4080

aagaacgagg aguacuucga guucguccag aaccgcaaca acaagcggcc cgccgccacg     4140

aagaaggccg ggcaggcgaa gaagaagaag uaaggacuag ugaagggaac acccaaauuu     4200

aauucagccu uaagcacaau uaauuaagag ugaaacguaa uuguacaagc aguuggucac     4260

ccaccauagg gcaugaucaa caccgcaacc uuuccuuuuu cccccaguga uucugaaaaa     4320

ccccucuucc cuucagcuug cuuagauguu ccaaauuuag uaagcuuaag gcggccuaca     4380

gaagaaaaag aaaaaaaagg ccacaaaagu ucccucucac uuucaguaaa uaaaauaaaa     4440

gcagcaacag aaauaaagaa auaaaugaaa uucaaaauga aauaaauauu guguugugca     4500

gcauuaaaaa aucaauaaaa auuaaaaaug agcaagaucu aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                 4609


<210>  2362
<211>  4497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2362
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc ggacaagaag uacagcaucg gccuggacau cgggaccaac      180

uccgugggcu gggccgugau caccgacgag uacaaggucc ccagcaagaa guucaaggug      240

cuggggaaca cggaccggca cuccaucaag aagaaccuga ucggcgcccu gcuguucgac      300

agcggcgaga ccgccgaggc cacccgccug aagcggaccg cgcgccggcg cuacacgcgg      360

cgcaagaacc ggaucugcua ccugcaggag aucuucucca acgagauggc caagguggac      420

gacagcuucu uccaccgccu cgaggagucc uuccuggucg aggaggacaa gaagcacgag      480

cggcacccca ucuucgggaa caucguggac gagguggccu accacgagaa guaccccacc      540

aucuaccacc uccgcaagaa gcucgucgac agcaccgaca aggccgaccu gcggcugauc      600

uaccucgcgc uggcccacau gaucaaguuc cgcggccacu uccugaucga gggggaccuc      660

aacccggaca acuccgacgu ggacaagcug uucauccagc uggugcagac cuacaaccag      720

cuguucgagg agaaccccau caacgccagc ggcgucgacg ccaaggccau ccuguccgcg      780

cggcugagca agucccgccg gcucgagaac cugaucgccc agcugcccgg ggagaagaag      840

aacggccucu ucggcaaccu gaucgcccug agccucgggc ugacgcccaa cuucaagucc      900

aacuucgacc uggccgagga cgccaagcuc cagcugagca aggacaccua cgacgacgac      960

cuggacaacc uccuggcgca gaucggcgac caguacgccg accuguuccu cgccgccaag     1020

aaccuguccg acgccauccu gcucagcgac auccugcgcg ugaacaccga gaucaccaag     1080

gcgccgcugu ccgccagcau gaucaagcgg uacgacgagc accaccagga ccucacgcug     1140

cugaaggccc ucgugcgcca gcagcugccc gagaaguaca aggagaucuu cuucgaccag     1200

agcaagaacg gguacgccgg cuacaucgac ggcggggccu cccaggagga guucuacaag     1260

uucaucaagc ccauccugga gaagauggac ggcaccgagg agcuccuggu caagcugaac     1320

cgggaggacc ugcuccgcaa gcagcgcacc uucgacaacg ggagcauccc ccaccagauc     1380

caccugggcg agcugcacgc gauccuccgg cgccaggagg acuucuaccc guuccugaag     1440

gacaaccggg agaagaucga gaagauccug accuuccgca uccccuacua cguggggccc     1500

cucgcccggg gcaacucccg cuucgccugg augacgcgca agagcgagga gaccaucacc     1560

cccuggaacu ucgaggaggu ggucgacaag ggcgccuccg cgcagagcuu caucgagcgg     1620

augaccaacu ucgacaagaa ccugccgaac gagaaggugc ugcccaagca cucccuccug     1680

uacgaguacu ucacggugua caacgagcug accaagguca aguacgugac cgaggggaug     1740

cggaagcccg ccuuccucag cggcgagcag aagaaggcca ucguggaccu gcuguucaag     1800

accaaccgca aggucacggu gaagcagcuc aaggaggacu acuucaagaa gaucgagugc     1860

uucgacuccg ucgagaucag cgggguggag gaccgguuca acgccucccu gggcaccuac     1920

cacgaccugc ucaagaucau caaggacaag gacuuccugg acaacgagga gaacgaggac     1980

auccuggagg acaucgugcu cacccugacc cuguucgagg accgcgagau gaucgaggag     2040

cggcucaaga cguacgccca ccuguucgac gacaagguca ugaagcagcu gaagcgccgg     2100

cgcuacaccg gguggggccg gcucagccgc aagcugauca acggcauccg ggacaagcag     2160

uccgggaaga ccauccugga cuuccucaag agcgacggcu ucgcgaaccg caacuucaug     2220

cagcugaucc acgacgacuc ccugaccuuc aaggaggaca uccagaaggc ccaggugagc     2280

gggcagggcg acucccucca cgagcacauc gccaaccugg ccgggagccc cgccaucaag     2340

aagggcaucc ugcagacggu gaaggucgug gacgagcucg ugaaggucau gggccgccac     2400

aagccggaga acaucgugau cgagauggcg cgggagaacc agaccaccca gaaggggcag     2460

aagaacuccc gcgagcggau gaagcgcauc gaggagggca ucaaggagcu ggggagccag     2520

auccugaagg agcaccccgu ggagaacacc cagcuccaga acgagaagcu guaccuguac     2580

uaccuccaga acggccggga cauguacguc gaccaggagc uggacaucaa ccgccugagc     2640

gacuacgacg uggaccacau cgugccccag uccuuccuca aggacgacag caucgacaac     2700

aagguccuga cgcgguccga caagaaccgc ggcaagagcg acaacgugcc cuccgaggag     2760

guggucaaga agaugaagaa cuacuggcgg cagcugcuca acgccaagcu gaucacccag     2820

cgcaaguucg acaaccugac caaggccgag cgcgggggcc ucagcgagcu ggacaaggcc     2880

ggguucauca agcggcagcu gguggagacc cggcagauca ccaagcacgu ggcccagauc     2940

cucgacuccc gcaugaacac gaaguacgac gagaacgaca agcugauccg ggaggucaag     3000

gugaucaccc ucaagagcaa gcucgugucc gacuuccgca aggacuucca guucuacaag     3060

guccgggaga ucaacaacua ccaccacgcg cacgacgccu accugaacgc cguggugggc     3120

accgcccuga ucaagaagua cccgaagcuc gagagcgagu ucgucuacgg ggacuacaag     3180

guguacgacg ugcgcaagau gaucgcgaag uccgagcagg agaucggcaa ggccaccgcc     3240

aaguacuucu ucuacagcaa caucaugaac uucuucaaga cggagaucac ccuggccaac     3300

ggcgagaucc ggaagcgccc ccugaucgag accaacgggg agaccggcga gaucgucugg     3360

gacaaggggc gggacuucgc cacggugcgc aaggugcucu ccaugcccca ggucaacauc     3420

gugaagaaga ccgaggugca gaccggcggg uucagcaagg aguccauccu gcccaagcgc     3480

aacagcgaca agcugaucgc gcggaagaag gacugggacc cgaagaagua cggcggcuuc     3540

gacuccccca ccgucgccua cagcgugcuc guggucgcca agguggagaa gggcaagagc     3600

aagaagcuga aguccgugaa ggagcugcuc gggaucacga ucauggagcg cagcuccuuc     3660

gagaagaacc ccaucgacuu ccuggaggcc aagggguaca aggaggucaa gaaggaccug     3720

aucaucaagc ucccgaagua cagccuguuc gagcuggaga acggccggaa gcgcaugcuc     3780

gccuccgcgg gggagcugca gaagggcaac gagcuggccc uccccagcaa guacgugaac     3840

uuccuguacc uggccuccca cuacgagaag cucaagggca gccccgagga caacgagcag     3900

aagcagcugu ucguggagca gcacaagcac uaccuggacg agaucaucga gcagaucucc     3960

gaguucagca agcgggucau ccucgccgac gccaaccugg acaaggugcu guccgcguac     4020

aacaagcacc gcgacaagcc cauccgggag caggccgaga acaucaucca ccucuucacc     4080

cugaccaacc ugggggcccc ggccgcguuc aaguacuucg acaccacgau cgaccgcaag     4140

cgguacacca gcaccaagga ggugcucgac gccacccuga uccaccaguc caucacgggc     4200

cuguacgaga cccgcaucga ccucagccag cucgggggcg acaagcggcc cgccgccacc     4260

aagaaggccg ggcaggcgaa gaagaagaag uaaggacuag uaauaaggaa acuguaugaa     4320

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4380

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4497


<210>  2363
<211>  5280
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2363
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gaacuucaag auccugccca ucgccaucga ccucggcgug      180

aagaacaccg ggguguucag cgccuucuac cagaagggca ccucccugga gcggcuggac      240

aacaagaacg ggaaggucua cgagcucagc aaggacuccu acacgcugcu gaugaacaac      300

cgcaccgccc ggcgccacca gcggcgcggc aucgaccgga agcagcucgu gaagcgccug      360

uucaagcuga ucuggaccga gcagcucaac cuggaguggg acaaggacac ccagcaggcc      420

aucagcuucc uguucaaccg gcgcggcuuc uccuucauca cggacgggua cagccccgag      480

uaccucaaca ucgugcccga gcaggucaag gcgauccuga uggacaucuu cgacgacuac      540

aacggcgagg acgaccugga cuccuaccuc aagcuggcca ccgagcagga gagcaagauc      600

uccgagaucu acaacaagcu gaugcagaag auccucgagu ucaagcugau gaagcugugc      660

accgacauca aggacgacaa ggugagcacc aagacgcuca aggagaucac cuccuacgag      720

uucgagcugc uggccgacua ccucgccaac uacagcgagu cccugaagac ccagaaguuc      780

agcuacaccg acaagcaggg gaaccugaag gagcucuccu acuaccacca cgacaaguac      840

aacauccagg aguuccugaa gcggcacgcg acgaucaacg accgcauccu ggacacccuc      900

cugaccgacg accuggacau cuggaacuuc aacuucgaga aguucgacuu cgacaagaac      960

gaggagaagc uccagaacca ggaggacaag gaccacaucc aggcccaccu ccaccacuuc     1020

guguucgccg ucaacaagau caagagcgag auggccuccg gcgggcggca ccgcagccag     1080

uacuuccagg agaucaccaa cgugcuggac gagaacaacc accaggaggg cuaccugaag     1140

aacuucugcg agaaccugca caacaagaag uacuccaacc ugagcgugaa gaaccucguc     1200

aaccugaucg gcaaccugag caaccucgag cugaagccgc ugcggaagua cuucaacgac     1260

aagauccacg ccaaggcgga ccacugggac gagcagaagu ucacggagac cuacugccac     1320

uggauccucg gggaguggcg cgugggcgug aaggaccagg acaagaagga cggggccaag     1380

uacuccuaca aggaccugug caacgagcug aagcagaagg ucaccaaggc cggccucgug     1440

gacuuccugc uggagcugga ccccugccgg accaucccgc ccuaccucga caacaacaac     1500

cgcaagccgc ccaagugcca gagccugauc cugaacccca aguuccucga caaccaguac     1560

cccaacuggc agcaguaccu gcaggagcug aagaagcucc aguccaucca gaacuaccug     1620

gacagcuucg agacggaccu gaaggugcuc aaguccagca aggaccagcc cuacuucguc     1680

gaguacaagu ccagcaacca gcagaucgcc uccggccagc gggacuacaa ggaccuggac     1740

gcccgcaucc ugcaguucau cuucgaccgg gugaaggcga gcgacgagcu ccugcugaac     1800

gagaucuacu uccaggccaa gaagcucaag cagaaggccu ccagcgagcu ggagaagcug     1860

gaguccagca agaagcucga cgaggugauc gccaacuccc agcugagcca gauccugaag     1920

ucccagcaca ccaacgggau cuucgagcag ggcaccuucc uccaccuggu cugcaaguac     1980

uacaagcagc gccagcgcgc ccgggacagc cgccuguaca ucaugcccga guaccgguac     2040

gacaagaagc uccacaagua caacaacacc gggcgcuucg acgacgacaa ccagcugcug     2100

acguacugca accacaagcc ccggcagaag cgcuaccagc uccugaacga ccuggcgggc     2160

gugcuccagg ugucccccaa cuuccugaag gacaagaucg ggagcgacga cgaccuguuc     2220

aucagcaagu ggcucgucga gcacauccgg ggcuucaaga aggccugcga ggacucccug     2280

aagauccaga aggacaaccg cggccugcuc aaccacaaga ucaacaucgc ccggaacacc     2340

aaggggaagu gcgagaagga gaucuucaac cugaucugca agaucgaggg cagcgaggac     2400

aagaagggga acuacaagca cggccuggcc uacgagcucg gggugcugcu guucggcgag     2460

ccgaacgagg ccuccaagcc cgaguucgac cgcaagauca agaaguucaa cagcaucuac     2520

uccuucgcgc agauccagca gaucgccuuc gccgagcgga agggcaacgc caacacgugc     2580

gcggucugca gcgccgacaa cgcccaccgc augcagcaga ucaagaucac cgagcccgug     2640

gaggacaaca aggacaagau cauccucucc gccaaggccc agcggcugcc cgcgaucccg     2700

acccgcaucg uggacggggc cgucaagaag auggccacca uccuggccaa gaacaucgug     2760

gacgacaacu ggcagaacau caagcagguc cucagcgcca agcaccagcu gcacaucccc     2820

aucaucaccg aguccaacgc guucgaguuc gagcccgccc uggccgacgu gaagggcaag     2880

agccucaagg accggcgcaa gaaggcccug gagcggaucu cccccgagaa caucuucaag     2940

gacaagaaca accgcaucaa ggaguucgcc aaggggauca gcgcguacuc cggcgccaac     3000

cugaccgacg gggacuucga cggcgccaag gaggagcucg accacaucau cccgcggagc     3060

cacaagaagu acggcacgcu gaacgacgag gccaaccuga ucugcgugac ccgcggggac     3120

aacaagaaca agggcaaccg caucuucugc cuccgggacc uggcggacaa cuacaagcug     3180

aagcaguucg agaccaccga cgaccucgag aucgagaaga agaucgccga cacgaucugg     3240

gacgccaaca agaaggacuu caaguucggg aacuaccgcu ccuucaucaa ccugacgccc     3300

caggagcaga aggccuuccg gcacgcccug uuccucgcgg acgagaaccc caucaagcag     3360

gccgugaucc gcgccaucaa caaccggaac cgcaccuucg ucaacggcac ccagcgguac     3420

uucgccgagg ugcuggccaa caacaucuac cugcgcgcga agaaggagaa ccucaacacc     3480

gacaagauca gcuucgacua cuucggcauc cccaccaucg ggaacggccg ggggaucgcc     3540

gagauccgcc agcuguacga gaagguggac uccgacaucc aggccuacgc caagggcgac     3600

aagccgcagg ccagcuacag ccaccugauc gacgcgaugc ucgccuucug caucgccgcc     3660

gacgagcacc ggaacgacgg guccaucggc cuggagaucg acaagaacua cagccuguac     3720

ccccucgaca agaacaccgg cgaggucuuc accaaggaca ucuucuccca gaucaagauc     3780

accgacaacg aguucagcga caagaagcug gugcgcaaga aggcgaucga gggguucaac     3840

acgcaccggc agaugacccg cgacggcauc uacgccgaga acuaccugcc cauccucauc     3900

cacaaggagc ugaacgaggu gcggaagggg uacaccugga agaacuccga ggagaucaag     3960

aucuucaagg gcaagaagua cgacauccag cagcugaaca accucgucua cugccugaag     4020

uucguggaca agccgaucag caucgacauc cagaucucca cccuggagga gcuccgcaac     4080

auccugacga ccaacaacau cgccgccacc gccgaguacu acuacaucaa ccugaagacc     4140

cagaagcucc acgaguacua caucgagaac uacaacacgg cgcuggggua caagaaguac     4200

agcaaggaga uggaguuccu gcggucccuc gccuaccgca gcgagcgggu gaagaucaag     4260

uccaucgacg acgucaagca gguccuggac aaggacagca acuucaucau cggcaagauc     4320

acccugcccu ucaagaagga guggcagcgc cucuaccggg aguggcagaa caccaccauc     4380

aaggacgacu acgaguuccu gaaguccuuc uucaacguca agagcaucac gaagcugcac     4440

aagaaggugc gcaaggacuu cucccucccc aucagcacca acgagggcaa guuccuggug     4500

aagcgcaaga ccugggacaa caacuucauc uaccagaucc ugaacgacuc cgacagccgg     4560

gccgacggga ccaagcccuu caucccggcc uucgacaucu ccaagaacga gaucguggag     4620

gccaucaucg acagcuucac gagcaagaac aucuucuggc uccccaagaa caucgagcug     4680

cagaaggugg acaacaagaa caucuucgcg aucgacaccu ccaagugguu cgagguggag     4740

acccccagcg accugcgcga caucggcauc gccaccaucc aguacaagau cgacaacaac     4800

ucccggccca aggugcgcgu caagcuggac uacgugaucg acgacgacag caagaucaac     4860

uacuucauga accacucccu ccucaagagc cgguacccgg acaaggugcu ggagauccug     4920

aagcagucca cgaucaucga guucgagagc uccggguuca acaagaccau caaggagaug     4980

cugggcauga agcuggccgg gaucuacaac gagaccagca acaacaagcg ccccgccgcc     5040

accaagaagg cgggccaggc caagaagaag aaguaaggac uaguaauaag gaaacuguau     5100

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     5160

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     5220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     5280


<210>  2364
<211>  3552
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2364
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gaagcggaac uacauccugg gccucgacau cgggaucacc      180

agcgugggcu acgggaucau cgacuacgag acccgcgacg ugaucgacgc cggcguccgg      240

cuguucaagg aggccaacgu ggagaacaac gagggccgcc gguccaagcg cggggcccgg      300

cgccugaagc ggcgccggcg ccaccggauc cagcgcguga agaagcuccu guucgacuac      360

aaccugcuca cggaccacag cgagcugucc ggcaucaacc ccuacgaggc ccgggucaag      420

gggcugagcc agaagcucuc cgaggaggag uucagcgcgg cccugcugca ccucgccaag      480

cgccggggcg ugcacaacgu gaacgagguc gaggaggaca ccgggaacga gcuguccacc      540

aaggagcaga ucagccgcaa cuccaaggcc cuggaggaga aguacguggc ggagcuccag      600

cuggagcggc ugaagaagga cggcgaggug cgcggcagca ucaaccgguu caagaccucc      660

gacuacguca aggaggccaa gcagcuccug aaggugcaga aggccuacca ccagcuggac      720

cagagcuuca ucgacacgua caucgaccuc cuggagaccc gccgcaccua cuacgagggg      780

cccggcgagg ggucccccuu cggcuggaag gacaucaagg agugguacga gaugcugaug      840

ggccacugca ccuacuuccc ggaggagcuc cggagcguga aguacgccua caacgccgac      900

cuguacaacg cgcugaacga ccucaacaac cuggucauca cgcgcgacga gaacgagaag      960

cuggaguacu acgagaaguu ccagaucauc gagaacgugu ucaagcagaa gaagaagccc     1020

acccucaagc agaucgccaa ggagauccug gugaacgagg aggacaucaa gggguaccgg     1080

gucaccucca ccggcaagcc cgaguucacg aaccugaagg uguaccacga caucaaggac     1140

aucaccgccc gcaaggagau caucgagaac gccgagcucc uggaccagau cgccaagauc     1200

cugaccaucu accagagcuc cgaggacauc caggaggagc ucaccaaccu gaacagcgag     1260

cugacgcagg aggagaucga gcagaucucc aaccucaagg gguacaccgg cacccacaac     1320

cugagccuga aggcgaucaa ccucauccug gacgagcugu ggcacaccaa cgacaaccag     1380

aucgccaucu ucaaccggcu caagcuggug cccaagaagg ucgaccugag ccagcagaag     1440

gagaucccga cgacccucgu ggacgacuuc auccuguccc ccguggucaa gcgcagcuuc     1500

auccagucca ucaaggugau caacgccauc aucaagaagu acgggcugcc caacgacauc     1560

aucaucgagc uggcccggga gaagaacagc aaggacgccc agaagaugau caacgagaug     1620

cagaagcgca accggcagac caacgagcgc aucgaggaga ucauccggac cacgggcaag     1680

gagaacgcga aguaccucau cgagaagauc aagcugcacg acaugcagga gggcaagugc     1740

cuguacuccc ucgaggccau cccccuggag gaccugcuca acaacccguu caacuacgag     1800

gucgaccaca ucaucccccg cagcgugucc uucgacaaca gcuucaacaa caaggugcug     1860

gucaagcagg aggagaacuc caagaagggg aaccggaccc ccuuccagua ccugagcucc     1920

agcgacucca agaucagcua cgagaccuuc aagaagcaca uccucaaccu ggccaagggc     1980

aaggggcgca ucuccaagac caagaaggag uaccugcucg aggagcggga caucaaccgc     2040

uucagcgugc agaaggacuu caucaaccgg aaccuggugg acacgcgcua cgccacccgg     2100

ggccugauga accuccugcg cuccuacuuc cgcgucaaca accuggacgu gaaggucaag     2160

agcaucaacg ggggcuucac cuccuuccuc cggcgcaagu ggaaguucaa gaaggagcgg     2220

aacaagggcu acaagcacca cgccgaggac gcgcugauca ucgccaacgc cgacuucauc     2280

uucaaggagu ggaagaagcu ggacaaggcc aagaagguga uggagaacca gauguucgag     2340

gagaagcagg cggagagcau gcccgagauc gagaccgagc aggaguacaa ggagaucuuc     2400

aucacgccgc accagaucaa gcacaucaag gacuucaagg acuacaagua cagccaccgc     2460

guggacaaga agcccaaccg ggagcucauc aacgacaccc uguacuccac ccgcaaggac     2520

gacaagggga acacccugau cgugaacaac cucaacggcc uguacgacaa ggacaacgac     2580

aagcugaaga agcucaucaa caagagcccc gagaagcugc ugauguacca ccacgacccc     2640

cagacguacc agaagcucaa gcugaucaug gagcaguacg gggacgagaa gaacccgcug     2700

uacaaguacu acgaggagac cggcaacuac cucaccaagu acuccaagaa ggacaacggg     2760

cccgucauca agaagaucaa guacuacggc aacaagcuga acgcccaccu ggacaucacc     2820

gacgacuacc ccaacagccg gaacaaggug gugaagcucu cccugaagcc cuaccgcuuc     2880

gacgucuacc uggacaacgg cguguacaag uucgugaccg ucaagaaccu cgacgugauc     2940

aagaaggaga acuacuacga ggugaacagc aagugcuacg aggaggccaa gaagcugaag     3000

aagaucucca accaggccga guucaucgcc agcuucuaca acaacgaccu gaucaagauc     3060

aacggggagc ucuaccgggu caucggcgug aacaacgacc ugcugaaccg caucgaggug     3120

aacaugaucg acaucacgua ccgggaguac cucgagaaca ugaacgacaa gcgcccgccc     3180

cggaucauca agaccaucgc guccaagacc cagagcauca agaaguacuc caccgacauc     3240

cuggggaacc uguacgaggu caagagcaag aagcaccccc agaucaucaa gaagggcaag     3300

cgccccgccg ccacgaagaa ggccggccag gccaagaaga agaaguaagg acuaguaaua     3360

aggaaacugu augaaugucu gugggcagga agugaagaga uccuucugua aagguuuuug     3420

gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua aaucugguag aaaaaugaga     3480

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaagaa uu                                                         3552


<210>  2365
<211>  4620
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2365
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gcuguucaac aagugcauca ucaucagcau caaccucgac      180

uucuccaaca aggagaagug caugaccaag cccuacagca ucggccugga caucgggacc      240

aacuccgugg gcugggccgu gaucacggac aacuacaagg uccccagcaa gaagaugaag      300

gugcugggga acaccuccaa gaaguacauc aagaagaacc uccugggcgu gcugcuguuc      360

gacagcggca ucaccgccga ggggcggcgc cugaagcgga ccgcccgccg gcgcuacacg      420

cggcgccgga accgcauccu guaccuccag gagaucuucu ccaccgagau ggccacccug      480

gacgacgcgu ucuuccagcg gcuggacgac agcuuccucg uccccgacga caagcgcgac      540

uccaaguacc cgaucuucgg caaccuggug gaggagaagg uguaccacga cgaguucccc      600

accaucuacc accuccggaa guaccucgcc gacagcacga agaaggccga ccugcgccug      660

gucuaccucg cccuggcgca caugaucaag uaccgggggc acuuccugau cgagggcgag      720

uucaacucca agaacaacga cauccagaag aacuuccagg acuuccucga caccuacaac      780

gccaucuucg agagcgaccu gucccuggag aacagcaagc agcucgagga gaucgugaag      840

gacaagaucu ccaagcugga gaagaaggac cgcauccuga agcuguuccc cggggagaag      900

aacagcggca ucuucuccga guuccugaag cugaucgugg gcaaccaggc cgacuuccgg      960

aagugcuuca accucgacga gaaggccagc cugcacuucu ccaaggagag cuacgacgag     1020

gaccuggaga cccuccuggg guacaucggc gacgacuaca gcgacgucuu ccugaaggcc     1080

aagaagcucu acgacgcgau ccugcugucc ggguuccuca ccgugacgga caacgagacc     1140

gaggcgcccc ugagcuccgc caugaucaag cgcuacaacg agcacaagga ggaccuggcc     1200

cuccugaagg aguacauccg gaacaucagc cugaagaccu acaacgaggu guucaaggac     1260

gacaccaaga acggcuacgc cggcuacauc gacgggaaga cgaaccagga ggacuucuac     1320

gucuaccuca agaaccugcu ggccgaguuc gagggcgccg acuacuuccu cgagaagauc     1380

gaccgcgagg acuuccugcg gaagcagcgc accuucgaca acggguccau cccguaccag     1440

auccaccucc aggagaugcg cgccauccug gacaagcagg ccaaguucua ccccuuccuc     1500

gccaagaaca aggagcggau cgagaagauc cugaccuucc gcauccccua cuacgugggc     1560

ccccuggcgc gggggaacag cgacuucgcc ugguccaucc gcaagcggaa cgagaagauc     1620

accccgugga acuucgagga cgugaucgac aaggagagcu ccgccgaggc cuucaucaac     1680

cgcaugacga gcuucgaccu cuaccugccc gaggagaagg uccugcccaa gcacucccuc     1740

cuguacgaga ccuucaacgu guacaacgag cugaccaagg ugcgguucau cgccgagagc     1800

augcgcgacu accaguuccu cgacuccaag cagaagaagg acaucguccg gcuguacuuc     1860

aaggacaagc gcaaggugac cgacaaggac aucaucgagu accugcacgc gaucuacggc     1920

uacgacggca ucgagcucaa ggggaucgag aagcaguuca acagcucccu gagcacguac     1980

cacgaccugc ucaacaucau caacgacaag gaguuccugg acgacuccag caacgaggcc     2040

aucaucgagg agaucaucca cacccugacc aucuucgagg accgggagau gaucaagcag     2100

cgccucucca aguucgagaa caucuucgac aagagcgugc ugaagaagcu gagccggcgc     2160

cacuacaccg gcugggggaa gcucuccgcc aagcugauca acggcauccg ggacgagaag     2220

uccgggaaca cgauccugga cuaccucauc gacgacggca ucagcaaccg caacuucaug     2280

cagcugaucc acgacgacgc ccugagcuuc aagaagaaga uccagaaggc gcagaucauc     2340

ggcgacgagg acaaggggaa caucaaggag gucgugaagu cccuccccgg cagcccggcc     2400

aucaagaagg ggauccugca guccaucaag aucgucgacg agcuggugaa ggugaugggc     2460

gggcggaagc ccgagagcau cgucguggag auggcccgcg agaaccagua caccaaccag     2520

ggcaagucca acagccagca gcggcucaag cgccuggaga agucccugaa ggagcucggc     2580

agcaagaucc ugaaggagaa cauccccgcc aagcugucca agaucgacaa caacgcccuc     2640

cagaacgacc gccuguaccu guacuaccuc cagaacggga aggacaugua caccggcgac     2700

gaccuggaca ucgaccggcu gagcaacuac gacaucgacc acaucauccc ccaggcguuc     2760

cucaaggaca acuccaucga caacaaggug cuggucagcu ccgccagcaa ccgcgggaag     2820

uccgacgacu ucccgagccu ggagguggug aagaagcgga agaccuucug guaccagcuc     2880

cucaagagca agcugaucuc ccagcgcaag uucgacaacc ugacgaaggc cgagcggggc     2940

ggccugcugc ccgaggacaa ggccggguuc auccagcgcc agcucgucga gacccggcag     3000

aucaccaagc acguggcccg ccugcuggac gagaaguuca acaacaagaa ggacgagaac     3060

aaccgggcgg ugcgcaccgu caagaucauc acgcucaaga gcacccuggu gucccaguuc     3120

cggaaggacu ucgagcugua caaggugcgc gagaucaacg acuuccacca cgcccacgac     3180

gccuaccuca acgccgucau cgccagcgcg cugcugaaga aguaccccaa gcucgagccc     3240

gaguucgugu acggcgacua cccgaaguac aacuccuucc gggagcgcaa gagcgccacc     3300

gagaaggugu acuucuacuc caacaucaug aacaucuuca agaaguccau cagccuggcc     3360

gacgggcggg ucaucgagcg cccccugauc gaggugaacg aggagaccgg cgagagcgug     3420

uggaacaagg aguccgaccu cgccaccguc cggcgcgugc ugagcuaccc ccaggugaac     3480

gucgugaaga agguggagga gcagaaccac gggcuggacc ggggcaagcc caagggccuc     3540

uucaacgcga accuguccag caagccgaag cccaacucca acgagaaccu ggucggggcc     3600

aaggaguacc ucgaccccaa gaaguacggc ggguacgccg gcaucagcaa cuccuucgcc     3660

gugcugguga aggggacgau cgagaagggc gccaagaaga agaucaccaa cguccuggag     3720

uuccagggca ucagcauccu cgaccgcauc aacuaccgga aggacaagcu gaacuuccug     3780

cucgagaagg gguacaagga caucgagcug aucaucgagc ugccgaagua cagccucuuc     3840

gagcuguccg acggcagccg ccgcaugcug gcguccaucc ucagcaccaa caacaagcgc     3900

ggggagaucc acaagggcaa ccagaucuuc cugucccaga aguucgugaa gcugcucuac     3960

cacgccaagc ggaucagcaa caccaucaac gagaaccacc ggaaguacgu ggagaaccac     4020

aagaaggagu ucgaggagcu guucuacuac auccuggagu ucaacgagaa cuacgucggg     4080

gccaagaaga acggcaagcu ccugaacucc gccuuccaga gcuggcagaa ccacuccauc     4140

gacgagcugu gcagcuccuu caucggcccc acggggagcg agcgcaaggg ccucuucgag     4200

cugaccuccc gggggagcgc cgcggacuuc gaguuccugg gcgugaagau cccccgcuac     4260

cgggacuaca cccccuccag ccuccugaag gacgccaccc ugauccacca guccgugacg     4320

gggcuguacg agacccgcau cgaccucgcc aagcugggcg agggcaagcg gccggccgcc     4380

accaagaagg cggggcaggc caagaagaag aaguaaggac uaguaauaag gaaacuguau     4440

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     4500

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     4620


<210>  2366
<211>  3696
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2366
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gugguacgcc agccugaugu ccgcccacca ccuccgggug      180

ggcaucgacg uggggaccca cagcgucggc cuggccaccc ugcgcgugga cgaccacggg      240

acgcccaucg agcuccuguc cgcccugagc cacauccacg acuccggcgu gggcaaggag      300

gggaagaagg accacgacac ccggaagaag cucagcggca ucgcgcgccg ggcccgccgg      360

cugcugcacc accgccggac ccagcuccag cagcuggacg agguccugcg cgaccucggg      420

uuccccaucc ccaccccggg cgaguuccug gaccugaacg agcagacgga ccccuaccgg      480

guguggcgcg ugcgggcccg ccucgucgag gagaagcugc ccgaggagcu gcgggggccc      540

gccaucucca uggcggugcg ccacaucgcc cggcaccgcg gcuggcggaa cccguacagc      600

aagguggagu cccuccugag ccccgccgag gagucccccu ucaugaaggc ccugcgcgag      660

cggauccucg ccaccaccgg cgagguccug gacgacggga ucacccccgg ccaggcgaug      720

gcccaggugg cccugacgca caacaucagc augcgcgggc cggagggcau ccucggcaag      780

cugcaccagu ccgacaacgc caacgagauc cgcaagaucu gcgcccggca gggggugagc      840

cccgacgucu gcaagcagcu gcuccgcgcg guguucaagg ccgacagccc ccggggcucc      900

gccguguccc gcgucgcccc cgacccgcug cccgggcagg gcagcuuccg gcgcgccccc      960

aagugcgacc ccgaguucca gcgguuccgc aucaucucca ucguggcgaa ccugcggauc     1020

agcgagacca agggggagaa ccgcccgcuc accgccgacg agcggcgcca cguggucacc     1080

uuccugacgg aggacagcca ggccgaccug accugggugg acguggccga gaagcucggc     1140

guccaccggc gcgaccugcg gggcaccgcc gugcacaccg acgacgggga gcgcuccgcg     1200

gcccggccgc ccaucgacgc cacggaccgc aucaugcggc agaccaagau cagcucccug     1260

aagaccuggu gggaggaggc cgacagcgag cagcgcggcg cgaugauccg cuaccucuac     1320

gaggacccca ccgacuccga gugcgccgag aucaucgccg agcugcccga ggaggaccag     1380

gccaagcugg acagccucca ccuccccgcc gggcgggcgg ccuacucccg cgagagccug     1440

acggcccugu ccgaccacau gcuggccacc accgacgacc ugcacgaggc ccggaagcgc     1500

cucuucggcg ucgacgacag cugggcgccg cccgccgagg ccaucaacgc ccccgugggg     1560

aaccccuccg uggaccggac ccugaagauc gucggccgcu accugagcgc cguggagucc     1620

auguggggca cgcccgaggu gauccacguc gagcacgugc gggacggguu caccagcgag     1680

cgcauggcgg acgagcggga caaggccaac cgccggcgcu acaacgacaa ccaggaggcc     1740

augaagaaga uccagcggga cuacggcaag gagggguaca ucucccgcgg cgacaucgug     1800

cggcucgacg cccuggagcu gcaggggugc gcgugccugu acugcggcac caccaucggc     1860

uaccacacgu gccagcucga ccacaucguc ccgcaggccg ggcccggcag caacaaccgc     1920

cgggggaacc ugguggccgu gugcgagcgc ugcaaccggu ccaagagcaa cacccccuuc     1980

gccgucuggg cccagaagug cggcaucccc cacgugggcg ugaaggaggc gaucgggcgc     2040

guccggggcu ggcgcaagca gaccccgaac accuccagcg aggaccugac gcgccucaag     2100

aaggagguga ucgcccggcu gcgccggacc caggaggacc ccgagaucga cgagcgcagc     2160

auggaguccg uggccuggau ggccaacgag cugcaccacc ggaucgccgc ggccuacccc     2220

gagaccaccg ucauggugua ccgcgggagc aucacggccg ccgcccggaa ggcggccggc     2280

aucgacuccc gcaucaaccu caucggggag aagggccgga aggaccgcau cgaccggcgc     2340

caccacgccg uggacgccag cgucguggcg cugauggagg ccuccguggc caagacccug     2400

gccgagcgga gcucccuccg cggcgagcag cggcugaccg ggaaggagca gaccuggaag     2460

caguacaccg gcagcacggu cggggcccgc gagcacuucg agauguggcg gggccacaug     2520

cugcaccuca ccgagcuguu caacgagcgc cuggcggagg acaaggugua cgugacccag     2580

aacauccggc uccgccuguc cgacgggaac gcccacaccg ucaaccccag caagcuggug     2640

ucccaccgcc ucggcgacgg ccugacggug cagcagaucg accgggccug caccccggcc     2700

cuguggugcg cccucacccg cgagaaggac uucgacgaga agaacgggcu gcccgcgcgg     2760

gaggaccgcg ccauccgggu ccacggccac gagaucaaga gcuccgacua cauccaggug     2820

uucagcaagc gcaagaagac cgacuccgac cgggacgaga cgcccuucgg ggccaucgcc     2880

gugcgcggcg gguucgucga gaucggcccc agcauccacc acgcccggau cuaccgcgug     2940

gagggcaaga agccggugua cgcgaugcug cgggucuuca cccacgaccu ccugucccag     3000

cgccacgggg accuguucag cgccgugauc ccgccccaga gcaucuccau gcggugcgcc     3060

gagcccaagc uccgcaaggc caucaccacc ggcaacgcca cguaccuggg gugggugguc     3120

gugggcgacg agcuggagau caacguggac agcuucacca aguacgcgau cgggcgguuc     3180

cucgaggacu uccccaacac cacccgcugg cggaucugcg gcuacgacac gaacuccaag     3240

cugacccuga agcccaucgu ccucgccgcc gagggccugg agaaccccag cuccgccgug     3300

aacgagaucg uggagcugaa gggguggcgc gucgcgauca acgugcucac caaggugcac     3360

cccaccgucg ugcggcgcga cgcccugggc cggccgcgcu acagcucccg cagcaaccug     3420

cccacguccu ggaccaucga gaagcggccc gccgccacca agaaggccgg gcaggcgaag     3480

aagaagaagu aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     3540

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     3600

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3696


<210>  2367
<211>  4557
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2367
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gaccaagccc uacagcaucg gccuggacau cgggaccaac      180

uccgugggcu gggccgugac gaccgacaac uacaaggucc ccagcaagaa gaugaaggug      240

cucgggaaca ccuccaagaa guacaucaag aagaaccugc ugggcgugcu ccuguucgac      300

agcggcauca ccgccgaggg gcggcgccug aagcggacgg cccgccggcg cuacacccgg      360

cgccggaacc gcauccucua ccugcaggag aucuucucca ccgagauggc cacccuggac      420

gacgcguucu uccagcggcu cgacgacagc uuccuggucc ccgacgacaa gcgcgacucc      480

aaguacccga ucuucggcaa ccugguggag gagaaggccu accacgacga guuccccacg      540

aucuaccacc uccggaagua ccuggccgac agcaccaaga aggccgaccu gcgccucgug      600

uaccuggcgc uggcccacau gaucaaguac cgggggcacu uccucaucga gggcgaguuc      660

aacuccaaga acaacgacau ccagaagaac uuccaggacu uccuggacac cuacaacgcc      720

aucuucgaga gcgaccuguc ccucgagaac agcaagcagc uggaggagau cgucaaggac      780

aagaucucca agcuggagaa gaaggaccgc auccucaagc uguuccccgg ggagaagaac      840

agcggcaucu ucuccgaguu ccugaagcuc aucgugggca accaggccga cuuccggaag      900

ugcuucaacc uggacgagaa ggccagccug cacuucucca aggagagcua cgacgaggac      960

cucgagaccc ugcuggggua caucggcgac gacuacuccg acguguuccu caaggcgaag     1020

aagcuguacg acgccauccu gcucagcggg uuccugacgg ucaccgacaa cgagaccgag     1080

gcgccccuga gcuccgccau gaucaagcgc uacaacgagc acaaggagga ccucgcccug     1140

cugaaggagu acauccggaa caucagccuc aagaccuaca acgagguguu caaggacgac     1200

acgaagaacg gcuacgccgg cuacaucgac gggaagacca accaggagga cuucuacgug     1260

uaccugaaga agcugcucgc cgaguucgag ggcgccgacu acuuccugga gaagaucgac     1320

cgcgaggacu uccugcggaa gcagcgcacc uucgacaacg gguccauccc guaccagauc     1380

caccuccagg agaugcgcgc cauccuggac aagcaggcca aguucuaccc cuuccuggcg     1440

aagaacaagg agcggaucga gaagauccug accuuccgca uccccuacua cgucggcccc     1500

cucgcccggg ggaacagcga cuucgccugg uccauccgca agcggaacga gaagaucacg     1560

ccguggaacu ucgaggacgu gaucgacaag gagagcuccg ccgaggccuu caucaaccgc     1620

augaccagcu ucgaccugua ccugcccgag gagaaggugc uccccaagca cucccugcug     1680

uacgagaccu ucaacgucua caacgagcuc accaaggugc gguucaucgc ggagagcaug     1740

cgcgacuacc aguuccugga cuccaagcag aagaaggaca ucgugcggcu guacuucaag     1800

gacaagcgca aggucacgga caaggacauc aucgaguacc uccacgccau cuacggcuac     1860

gacggcaucg agcugaaggg gaucgagaag caguucaaca gcucccugag caccuaccac     1920

gaccuccuga acaucaucaa cgacaaggag uuccuggacg acuccagcaa cgaggccauc     1980

aucgaggaga ucauccacac ccucaccauc uucgaggacc gggagaugau caagcagcgc     2040

cuguccaagu ucgagaacau cuucgacaag agcgugcuga agaagcucuc ccggcgccac     2100

uacacgggcu gggggaagcu gagcgccaag cugaucaacg gcauccggga cgagaagucc     2160

gggaacacca uccucgacua ccugaucgac gacggcauca gcaaccgcaa cuucaugcag     2220

cugauccacg acgacgcgcu cagcuucaag aagaagaucc agaaggccca gaucaucggc     2280

gacgaggaca aggggaacau caaggaggug gucaaguccc ugcccggcag cccggccauc     2340

aagaagggga uccugcaguc caucaagauc guggacgagc ucgucaaggu gaugggcggg     2400

cggaagcccg agagcaucgu ggucgagaug gcccgcgaga accaguacac caaccagggc     2460

aaguccaaca gccagcagcg gcugaagcgc cuggagaagu cccucaagga gcugggcagc     2520

aagauccuga aggagaacau ccccgccaag cucuccaaga ucgacaacaa cgcgcugcag     2580

aacgaccgcc uguaccucua cuaccugcag aacgggaagg acauguacac cggcgacgac     2640

cuggacaucg accggcucag caacuacgac aucgaccaca ucauccccca ggccuuccug     2700

aaggacaacu ccaucgacaa caaggugcug gugagcuccg ccagcaaccg cgggaagucc     2760

gacgacgucc cgagccucga gguggugaag aagcggaaga cguucuggua ccagcugcuc     2820

aagagcaagc ugaucuccca gcgcaaguuc gacaaccuga ccaaggccga gcggggcggc     2880

cugagccccg aggacaaggc cggguucauc cagcgccagc ucgucgagac ccggcagauc     2940

accaagcacg uggcgcgccu gcuggacgag aaguucaaca acaagaagga cgagaacaac     3000

cgggccgugc gcacggucaa gaucaucacc cucaagucca cccuggugag ccaguuccgg     3060

aaggacuucg agcuguacaa ggugcgcgag aucaacgacu uccaccacgc ccacgacgcc     3120

uaccucaacg ccgucguggc guccgcccug cugaagaagu accccaagcu cgagcccgag     3180

uucguguacg gcgacuaccc gaaguacaac agcuuccggg agcgcaaguc cgccaccgag     3240

aaggucuacu ucuacagcaa caucaugaac aucuucaaga aguccaucag ccuggccgac     3300

gggcggguga ucgagcgccc ccugaucgag gugaacgagg agaccggcga guccgucugg     3360

aacaaggaga gcgaccucgc gacggugcgg cgcgugcugu ccuaccccca ggucaacgug     3420

gugaagaagg ucgaggagca gaaccacggg cuggaccggg gcaagcccaa gggccucuuc     3480

aacgccaacc ugagcuccaa gccgaagccc aacagcaacg agaaccuggu gggggccaag     3540

gaguaccucg accccaagaa guacggcggg uacgccggca ucuccaacag cuucaccgug     3600

cuggucaagg ggaccaucga gaagggcgcc aagaagaaga ucaccaacgu gcuggaguuc     3660

cagggcaucu ccauccucga ccgcaucaac uaccggaagg acaagcugaa cuuccugcuc     3720

gagaaggggu acaaggacau cgagcugauc aucgagcugc cgaaguacag ccucuucgag     3780

cugagcgacg gcucccgccg caugcuggcg agcauccucu ccacgaacaa caagcgcggg     3840

gagauccaca agggcaacca gaucuuccug agccagaagu ucgugaagcu gcucuaccac     3900

gccaagcgga ucuccaacac caucaacgag aaccaccgga aguacgucga gaaccacaag     3960

aaggaguucg aggagcuguu cuacuacauc cuggaguuca acgagaacua cgugggggcc     4020

aagaagaacg gcaagcuccu gaacagcgcc uuccaguccu ggcagaacca cagcaucgac     4080

gagcugugcu ccagcuucau cggccccacc ggguccgagc gcaagggccu cuucgagcug     4140

accagccggg gguccgccgc ggacuucgag uuccugggcg ugaagauccc ccgcuaccgg     4200

gacuacacgc ccagcucccu ccugaaggac gccacccuga uccaccagag cgucaccggg     4260

cucuacgaga cccgcaucga ccuggccaag cugggcgagg gcaagcggcc ggccgccacg     4320

aagaaggcgg ggcaggccaa gaagaagaag uaaggacuag uaauaaggaa acuguaugaa     4380

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4440

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4557


<210>  2368
<211>  3639
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2368
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc ggccgccuuc aagcccaaca gcaucaacua cauccugggc      180

cucgacaucg ggaucgccuc cgugggcugg gccauggugg agaucgacga ggaggagaac      240

cccauccggc ugaucgaccu ggggguccgc guguucgagc gggcggaggu gcccaagacc      300

ggcgacagcc ucgccauggc ccgccggcug gcccgcuccg uccggcgccu gacccggcgc      360

cgggcgcacc gccuccugcg gacgcgccgg cugcucaagc gcgagggcgu gcugcaggcc      420

gccaacuucg acgagaacgg gcugaucaag agccucccga acacccccug gcagcugcgg      480

gccgccgcgc uggaccgcaa gcucacgccc cuggaguggu ccgccgugcu gcuccaccug      540

aucaagcacc ggggcuaccu gagccagcgc aagaacgagg gggagaccgc cgacaaggag      600

cucggcgccc ugcugaaggg ggucgccggc aacgcgcacg cccuccagac cggcgacuuc      660

cggacccccg ccgagcuggc ccugaacaag uucgagaagg aguccgggca cauccgcaac      720

cagcgcagcg acuacuccca caccuucagc cggaaggacc uccaggccga gcugauccuc      780

cuguucgaga agcagaagga guucggcaac ccgcacgugu ccgggggccu gaaggagggc      840

aucgagaccc ugcucaugac gcagcgcccc gcgcugagcg gggacgccgu gcagaagaug      900

cugggccacu gcaccuucga gcccgccgag cccaaggccg ccaagaacac cuacaccgcg      960

gagcgguuca ucuggcucac gaagcugaac aaccugcgca uccucgagca gggguccgag     1020

cggccgcuga ccgacaccga gcgcgccacc cugauggacg agcccuaccg caagagcaag     1080

cucacguacg cccaggcccg gaagcugcug ggccucgagg acaccgcguu cuucaagggg     1140

cugcgguacg gcaaggacaa cgccgaggcc uccacccuga uggagaugaa ggccuaccac     1200

gccaucagcc gcgcgcucga gaaggagggc cugaaggaca agaagucccc gcugaaccug     1260

agccccgagc uccaggacga gaucgggacc gccuucuccc uguucaagac ggacgaggac     1320

aucaccggcc ggcugaagga ccgcauccag ccggagaucc ucgaggcccu gcugaagcac     1380

aucagcuucg acaaguucgu ccagaucagc cucaaggccc ugcggcgcau cgugccccug     1440

auggagcagg ggaagcggua cgacgaggcc ugcgcggaga ucuacggcga ccacuacggg     1500

aagaagaaca ccgaggagaa gaucuaccuc ccgcccaucc ccgccgacga gauccgcaac     1560

cccguggucc ugcgggcccu gucccaggcc cgcaagguga ucaacggcgu gguccggcgc     1620

uacggcagcc ccgcccgcau ccacaucgag accgcgcggg agguggggaa guccuucaag     1680

gaccgcaagg agaucgagaa gcggcaggag gagaaccgca aggaccggga gaaggccgcc     1740

gccaaguucc gcgaguacuu ccccaacuuc gugggcgagc ccaagagcaa ggacauccuc     1800

aagcugcggc uguacgagca gcagcacggg aagugccucu acuccggcaa ggagaucaac     1860

cuggggcgcc ugaacgagaa gggcuacguc gagaucgacc acgcgcuccc cuucagccgg     1920

acgugggacg acuccuucaa caacaaggug cuggugcugg gcagcgagaa ccagaacaag     1980

gggaaccaga cccccuacga guacuucaac ggcaaggaca acucccgcga guggcaggag     2040

uucaaggccc gggucgagac cagccgcuuc ccccggucca agaagcagcg cauccuccug     2100

cagaaguucg acgaggacgg guucaaggag cggaaccuga acgacacccg cuacgugaac     2160

cgguuccucu gccaguucgu cgccgaccgc augcggcuga cgggcaaggg caagaagcgc     2220

guguucgcca gcaacgggca gaucaccaac cugcuccggg gcuucugggg gcugcgcaag     2280

gugcgcgccg agaacgaccg gcaccacgcg cuggacgccg ucgugguggc cugcuccacc     2340

gucgccaugc agcagaagau cacccgcuuc gugcgguaca aggagaugaa cgccuucgac     2400

ggcaagacga ucgacaagga gaccggggag gugcuccacc agaagaccca cuucccgcag     2460

cccugggagu ucuucgcgca ggaggucaug auccgcgugu ucggcaagcc cgacggcaag     2520

ccggaguucg aggaggccga cacccuggag aagcugcgga cgcuccuggc cgagaagcug     2580

agcucccgcc ccgaggccgu gcacgaguac gucacgcccc ucuucgugag ccgggccccc     2640

aaccgcaaga uguccgggca gggccacaug gagaccguca agagcgcgaa gcggcuggac     2700

gagggggugu ccgugcugcg cgugccgcuc acccagcuga agcugaagga ccucgagaag     2760

auggucaacc gggagcgcga gcccaagcug uacgaggccc ugaaggcccg gcucgaggcc     2820

cacaaggacg accccgcgaa ggccuucgcc gagcccuucu acaaguacga caaggccggc     2880

aaccgcaccc agcaggucaa ggccgugcgg guggagcagg uccagaagac cggggugugg     2940

gugcgcaacc acaacggcau cgcggacaac gccaccaugg ugcgggugga cgucuucgag     3000

aagggcgaca aguacuaccu ggugccgauc uacagcuggc aggucgccaa ggggauccug     3060

cccgaccgcg ccguggugca gggcaaggac gaggaggacu ggcagcucau cgacgacagc     3120

uucaacuuca aguucucccu gcaccccaac gaccuggugg aggucaucac caagaaggcc     3180

cggauguucg gguacuucgc gagcugccac cgcggcaccg ggaacaucaa cauccgcauc     3240

cacgaccucg accacaagau cggcaagaac ggcauccugg aggggaucgg cgugaagacg     3300

gcccuguccu uccagaagua ccagaucgac gagcucggga aggagauccg gcccugccgc     3360

cugaagaagc ggccgcccgu gcgcaagcgg cccgccgcca ccaagaaggc cggccaggcg     3420

aagaagaaga aguaaggacu aguaauaagg aaacuguaug aaugucugug ggcaggaagu     3480

gaagagaucc uucuguaaag guuuuuggaa uuaugucugc ugaauaauaa acuuuuuuga     3540

aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            3639


<210>  2369
<211>  4395
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2369
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gaagaagccc uacaccaucg gccuggacau cgggaccaac      180

agcgugggcu gggccgugcu cacggaccag uacgaccugg ucaagcggaa gaugaagauc      240

gccggggacu ccgagaagaa gcagaucaag aagaacuucu ggggcgugcg ccuguucgac      300

gagggccaga ccgccgccga ccggcgcaug gcgcggaccg cccgccggcg caucgagcgg      360

cgccggaacc gcaucagcua ccuccagggg aucuucgccg aggagauguc caagaccgac      420

gccaacuucu ucugccggcu gagcgacucc uucuacgugg acaacgagaa gcgcaacagc      480

cggcaccccu ucuucgcgac gaucgaggag gaggucgagu accacaagaa cuaccccacc      540

aucuaccacc uccgcgagga gcuggugaac uccagcgaga aggccgaccu gcggcuggug      600

uaccucgccc uggcccacau caucaaguac cgcggcaacu uccugaucga gggggcccuc      660

gacacccaga acaccuccgu cgacggcauc uacaagcagu ucauccagac guacaaccag      720

guguucgcga gcgggaucga ggacggcucc cugaagaagc uggaggacaa caaggacgug      780

gccaagaucc ucgucgagaa ggugacccgg aaggagaagc uggagcgcau ccugaagcuc      840

uacccgggcg agaaguccgc cgggauguuc gcccaguuca ucagccugau cgugggcagc      900

aaggggaacu uccagaagcc cuucgaccug aucgagaagu ccgacaucga gugcgccaag      960

gacagcuacg aggaggaccu cgagucccug cuggcgcuca ucggcgacga guacgccgag     1020

cuguucgucg ccgccaagaa cgccuacagc gcgguggugc uguccagcau caucaccguc     1080

gccgagaccg agacgaacgc caagcucagc gccuccauga ucgagcgguu cgacacccac     1140

gaggaggacc ugggcgagcu gaaggccuuc aucaagcucc accuccccaa gcacuacgag     1200

gagaucuuca gcaacaccga gaagcacggg uacgcgggcu acaucgacgg gaagaccaag     1260

caggccgacu ucuacaagua caugaagaug acgcuggaga acaucgaggg cgccgacuac     1320

uucaucgcca agaucgagaa ggagaacuuc cugcgcaagc agcgcaccuu cgacaacggg     1380

gcgauccccc accagcugca ccuggaggag cucgaggcca uccugcacca gcaggccaag     1440

uacuacccgu uccugaagga gaacuacgac aagaucaagu cccucgugac cuuccggauc     1500

cccuacuucg ugggcccccu ggccaacggc cagagcgagu ucgccuggcu gacccgcaag     1560

gcggacgggg agauccggcc cuggaacauc gaggagaagg ucgacuucgg caaguccgcc     1620

guggacuuca ucgagaagau gacgaacaag gacaccuacc ucccgaagga gaacgugcug     1680

cccaagcaca gccugugcua ccagaaguac cucgucuaca acgagcugac caaggugcgc     1740

uacaucaacg accaggggaa gaccuccuac uucagcggcc aggagaagga gcagaucuuc     1800

aacgaccugu ucaagcagaa gcggaagguc aagaagaagg accucgagcu guuccugcgc     1860

aacauguccc acguggagag ccccacgauc gaggggcugg aggacuccuu caacagcucc     1920

uacagcaccu accacgaccu ccugaaggug ggcaucaagc aggagauccu ggacaacccc     1980

gucaacaccg agaugcucga gaacaucgug aagauccuga ccguguucga ggacaagcgg     2040

augaucaagg agcagcugca gcaguucucc gacguccucg acggcguggu gcugaagaag     2100

cuggagcgcc ggcacuacac gggguggggc cgccucagcg ccaagcugcu gauggggauc     2160

cgggacaagc agucccaccu caccauccug gacuaccuga ugaacgacga cggccucaac     2220

cgcaaccuga ugcagcugau caacgacagc aaccucuccu ucaagagcau caucgagaag     2280

gagcagguca ccaccgccga caaggacauc cagagcaucg uggccgaccu ggcggggucc     2340

ccggccauca agaagggcau ccugcagagc cucaagaucg uggacgagcu ggucuccgug     2400

augggcuacc cgccccagac gaucgugguc gagauggccc gggagaacca gaccaccggg     2460

aagggcaaga acaacagccg cccccgguac aagucccugg agaaggccau caaggaguuc     2520

gggagccaga uccucaagga gcaccccacc gacaaccagg agcugcgcaa caaccggcug     2580

uaccucuacu accugcagaa cggcaaggac auguacacgg gccaggaccu ggacauccac     2640

aaccucucca acuacgacau cgaccacauc gugccccaga gcuucaucac cgacaacucc     2700

aucgacaacc uggugcugac cagcuccgcc gggaaccgcg agaagggcga cgacgucccg     2760

ccccucgaga ucgugcggaa gcgcaaggug uucugggaga agcuguacca ggggaaccug     2820

augagcaagc gcaaguucga cuaccucacc aaggcggagc ggggcgggcu gaccgaggcc     2880

gacaaggccc gcuucaucca ccggcagcug gucgagacgc gccagaucac caagaacgug     2940

gccaacaucc uccaccagcg guucaacuac gagaaggacg accacggcaa caccaugaag     3000

cagguccgca ucgugacccu gaaguccgcg cuggugagcc aguuccggaa gcaguuccag     3060

cucuacaagg ugcgcgacgu caacgacuac caccacgccc acgacgccua ccugaacggc     3120

gugguggcca acacgcugcu caaggucuac ccccagcugg agcccgaguu cguguacggg     3180

gacuaccacc aguucgacug guucaaggcc aacaaggcga ccgccaagaa gcaguucuac     3240

accaacauca ugcuguucuu cgcccagaag gaccggauca ucgacgagaa cggcgagauc     3300

cucugggaca agaaguaccu ggacaccgug aagaagguca uguccuaccg ccagaugaac     3360

aucgugaaga agacggagau ccagaagggg gaguucagca aggccaccau caagcccaag     3420

ggcaacucca gcaagcugau cccccggaag accaacuggg acccgaugaa guacgggggc     3480

cucgacagcc ccaacauggc cuacgcggug gucaucgagu acgccaaggg caagaacaag     3540

cugguguucg agaagaagau cauccgcgug accaucaugg agcggaaggc cuucgagaag     3600

gacgagaagg ccuuccugga ggagcagggg uaccgccagc ccaagguccu cgccaagcug     3660

ccgaaguaca cgcuguacga gugcgaggag ggccggcgcc ggaugcucgc guccgccaac     3720

gaggcccaga aggggaacca gcagguccug cccaaccacc uggugacccu ccugcaccac     3780

gccgcgaacu gcgaggugag cgacggcaag ucccuggacu acaucgagag caaccgcgag     3840

auguucgccg agcuccuggc ccacgugucc gaguucgcca agcgguacac ccuggccgag     3900

gcgaaccuca acaagaucaa ccagcuguuc gagcagaaca aggaggggga caucaaggcc     3960

aucgcccaga gcuucgugga ccugauggcc uucaacgcca ugggcgcgcc cgccuccuuc     4020

aaguucuucg agaccacgau cgagcgcaag cgguacaaca accucaagga gcugcugaac     4080

agcaccauca ucuaccaguc caucaccggc cucuacgaga gccgcaagcg ccuggacgac     4140

aagcggcccg ccgccaccaa gaaggccggg caggcgaaga agaagaagua aggacuagua     4200

auaaggaaac uguaugaaug ucugugggca ggaagugaag agauccuucu guaaagguuu     4260

uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua auaaaucugg uagaaaaaug     4320

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa gaauu                                                      4395


<210>  2370
<211>  4428
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2370
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gaagaagccc uacagcaucg gccuggacau cgggaccaac      180

uccgugggcu gggccguggu caccgacgac uacaaggugc ccgccaagaa gaugaaggug      240

cucgggaaca cggacaagag ccacaucgag aagaaccugc ugggcgcccu ccuguucgac      300

uccggcaaca ccgccgagga ccggcgccug aagcggaccg cgcgccggcg cuacacccgg      360

cgccggaacc gcauccucua ccugcaggag aucuucagcg aggagauggg gaaggucgac      420

gacuccuucu uccaccggcu ggaggacagc uuccucguga cggaggacaa gcgcggcgag      480

cggcacccca ucuucgggaa ccuggaggag gaggugaagu accacgagaa cuucccgacc      540

aucuaccacc uccgccagua ccuggccgac aaccccgaga aggucgaccu gcggcuggug      600

uaccucgccc uggcccacau caucaaguuc cgcggccacu uccugaucga ggggaaguuc      660

gacacccgga acaacgacgu gcagcgccuc uuccaggagu uccuggcggu cuacgacaac      720

accuucgaga acuccagccu gcaggagcag aacguccagg uggaggagau ccucacggac      780

aagaucucca agagcgccaa gaaggaccgg gugcugaagc uguuccccaa cgagaagucc      840

aacggccgcu ucgccgaguu ccucaagcug aucgugggca accaggccga cuucaagaag      900

cacuucgagc uggaggagaa ggccccgcuc caguucagca aggacaccua cgaggaggag      960

cuggaggugc ugcucgcgca gaucggggac aacuacgccg agcuguuccu guccgccaag     1020

aagcucuacg acagcauccu gcuguccggc auccucaccg ucaccgacgu ggggacgaag     1080

gccccgcuga gcgccuccau gauccagcgg uacaacgagc accagaugga ccuggcgcag     1140

cucaagcagu ucauccgcca gaagcugagc gacaaguaca acgagguguu cuccgacguc     1200

agcaaggacg gcuacgccgg cuacaucgac gggaagacca accaggaggc cuucuacaag     1260

uaccugaagg gccuccugaa caagaucgag gggagcggcu acuuccugga caagaucgag     1320

cgcgaggacu uccuccggaa gcagcgcacc uucgacaacg gguccauccc ccaccagauc     1380

caccuccagg agaugcgggc caucauccgc cggcaggccg aguucuaccc cuuccuggcg     1440

gacaaccagg accgcaucga gaagcugcug accuuccgga uccccuacua cgugggcccg     1500

cuggcccgcg gcaagagcga cuucgccugg cugucccgca agagcgccga caagaucacg     1560

cccuggaacu ucgacgagau cguggacaag gaguccagcg ccgaggcguu caucaaccgg     1620

augaccaacu acgaccucua ccugcccaac cagaaggucc ugcccaagca cucccuccug     1680

uacgagaagu ucaccgugua caacgagcug accaagguca aguacaagac ggagcagggg     1740

aagaccgccu ucuucgacgc caacaugaag caggagaucu ucgacggcgu guucaaggug     1800

uaccggaagg ucaccaagga caagcucaug gacuuccugg agaaggaguu cgacgaguuc     1860

cgcaucgugg accugaccgg gcucgacaag gagaacaagg uguucaacgc cagcuacggc     1920

acguaccacg accugugcaa gauccuggac aaggacuucc ucgacaacuc caagaacgag     1980

aagauccugg aggacaucgu ccugacccuc acccuguucg aggaccggga gaugauccgc     2040

aagcggcugg agaacuacag cgaccuccug accaaggagc aggucaagaa gcuggagcgc     2100

cggcacuaca cggggugggg ccgccucucc gcggagcuga uccacggcau ccggaacaag     2160

gagagccgca agaccauccu ggacuaccuc aucgacgacg ggaacuccaa ccgcaacuuc     2220

augcagcuga ucaacgacga cgcccugagc uucaaggagg agaucgccaa ggcccaggug     2280

aucggcgaga ccgacaaccu caaccaggug guguccgaca ucgccgggag cccggcgauc     2340

aagaagggca uccugcaguc ccugaagauc guggacgagc ucgucaagau cauggggcac     2400

cagcccgaga acaucguggu ggagauggcc cgggagaacc aguucaccaa ccagggccgc     2460

cggaacagcc agcagcgccu gaagggccug acggacucca ucaaggaguu cgggagccag     2520

auccucaagg agcaccccgu cgagaacagc cagcugcaga acgaccggcu guuccucuac     2580

uaccugcaga acggccgcga cauguacacc ggggaggagc uggacaucga cuaccucucc     2640

caguacgaca ucgaccacau caucccccag gccuucauca aggacaacag caucgacaac     2700

cgggugcuga ccuccagcaa ggagaaccgc ggcaaguccg acgacgugcc gagcaaggac     2760

gucgugcgga agaugaaguc cuacuggagc aagcugcucu ccgccaagcu gaucacccag     2820

cgcaaguucg acaaccugac gaaggccgag cggggcgggc ucaccgacga cgacaaggcg     2880

ggcuucauca agcgccagcu gguggagacc cggcagauca ccaagcacgu cgcccgcauc     2940

cuggacgagc gguucaacac cgagacggac gagaacaaca agaagauccg ccaggugaag     3000

aucgugaccc ucaagagcaa ccuggucucc aacuuccgga aggaguucga gcuguacaag     3060

gugcgcgaga ucaacgacua ccaccacgcc cacgacgccu accucaacgc cgugaucggg     3120

aaggcgcugc ugggcgucua cccccagcuc gagcccgagu ucguguacgg ggacuacccc     3180

cacuuccacg gccacaagga gaacaaggcc accgccaaga aguucuucua cagcaacauc     3240

augaacuucu ucaagaagga cgacgugcgg accgacaaga acggcgagau caucuggaag     3300

aaggacgagc acaucuccaa caucaagaag guccugagcu acccccaggu gaacaucgug     3360

aagaaggucg aggagcagac cgggggcuuc uccaaggaga gcauccugcc caaggggaac     3420

uccgacaagc ucaucccccg caagacgaag aaguucuacu gggacaccaa gaaguacggc     3480

ggguucgaca gccccaucgu ggccuacagc auccugguga ucgcggacau cgagaagggc     3540

aaguccaaga agcugaagac cgucaaggcc cucgugggcg ugacgaucau ggagaagaug     3600

accuucgagc gggacccggu cgccuuccug gagcgcaagg gguaccgcaa cgugcaggag     3660

gagaacauca ucaagcugcc caaguacagc cucuucaagc uggagaacgg ccggaagcgc     3720

cugcucgccu ccgcccggga gcugcagaag gggaacgaga ucgugcugcc caaccaccuc     3780

ggcacccugc uguaccacgc gaagaacauc cacaaggucg acgagccgaa gcaccucgac     3840

uacguggaca agcacaagga cgaguucaag gagcugcugg acguggucag caacuucucc     3900

aagaaguaca cccucgccga ggggaaccug gagaagauca aggagcugua cgcccagaac     3960

aacggcgagg accucaagga gcuggccagc uccuucauca accugcucac guucaccgcc     4020

aucggcgcgc ccgccaccuu caaguucuuc gacaagaaca ucgaccgcaa gcgguacacc     4080

agcacgaccg agauccugaa cgccacccug auccaccagu ccaucaccgg gcucuacgag     4140

acgcgcaucg accugaacaa gcugggcggg gacaagcggc ccgccgccac caagaaggcg     4200

ggccaggcca agaagaagaa guaaggacua guaauaagga aacuguauga augucugugg     4260

gcaggaagug aagagauccu ucuguaaagg uuuuuggaau uaugucugcu gaauaauaaa     4320

cuuuuuugaa auaauaaauc ugguagaaaa augagaucua aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  4428


<210>  2371
<211>  3561
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2371
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gcagaccacc aaccugagcu acauccucgg ccuggaccug      180

gggaucgccu ccgugggcug ggccgugguc gagaucaacg agaacgagga ccccaucggg      240

cucaucgacg ugggcgugcg gaucuucgag cgcgccgagg uccccaagac gggcgagagc      300

cuggcccugu cccggcgccu cgcgcggagc acccgccggc ugauccgccg gcgcgcccac      360

cggcugcucc uggccaagcg cuuccugaag cgggagggga uccucuccac caucgaccug      420

gagaagggcc ugcccaacca ggccugggag cuccgcgugg cggggcugga gcggcgccug      480

agcgccaucg aguggggcgc cgugcuccug caccugauca agcaccgggg guaccucucc      540

aagcgcaaga acgagagcca gaccaacaac aaggagcugg gcgcccugcu cuccggcguc      600

gcccagaacc accagcugcu gcagagcgac gacuaccgga cgccggcgga gcucgcccug      660

aagaaguucg ccaaggagga ggggcacauc cgcaaccagc ggggcgccua cacccacacc      720

uucaaccgcc uggaccuccu ggccgagcug aaccuccugu ucgcgcagca gcaccaguuc      780

gggaaccccc acugcaagga gcacauccag caguacauga ccgagcugcu cauguggcag      840

aagcccgccc uguccggcga ggccauccug aagaugcucg gcaagugcac gcacgagaag      900

aacgaguuca aggccgccaa gcacaccuac agcgcggagc gcuucgugug gcugaccaag      960

cugaacaacc uccggauccu ggaggacggg gccgagcgcg cccugaacga ggaggagcgg     1020

cagcuccuga ucaaccaccc cuacgagaag uccaagcuga ccuacgccca ggugcgcaag     1080

cuccugggcc ugagcgagca ggccaucuuc aagcaccucc gguacuccaa ggagaacgcg     1140

gagagcgcca cguucaugga gcucaaggcc uggcacgcca uccgcaaggc gcuggagaac     1200

caggggcuga aggacaccug gcaggaccuc gccaagaagc cggaccugcu ggacgagauc     1260

ggcaccgccu ucucccucua caagaccgac gaggacaucc agcaguaccu gacgaacaag     1320

guccccaaca gcgugaucaa cgcccugcuc gugucccuga acuucgacaa guucaucgag     1380

cugagccuca agagccugcg gaagauccug ccccucaugg agcaggggaa gcgcuacgac     1440

caggccugcc gggagaucua cggccaccac uacggcgagg cgaaccagaa gaccucccag     1500

cugcugcccg ccaucccggc ccaggagauc cgcaaccccg ucgugcuccg gacccugagc     1560

caggcccgca aggugaucaa cgccaucauc cggcaguacg ggucccccgc gcgcguccac     1620

aucgagaccg gccgggagcu gggcaagagc uucaaggagc gccgggagau ccagaagcag     1680

caggaggaca accgcacgaa gcgggagucc gccgugcaga aguucaagga gcuguucagc     1740

gacuucucca gcgagcccaa guccaaggac auccugaagu uccgccugua cgagcagcag     1800

cacgggaagu gccucuacag cgggaaggag aucaacaucc accgccugaa cgagaagggc     1860

uacguggaga ucgaccacgc ccugccguuc ucccggaccu gggacgacag cuucaacaac     1920

aagguccucg ugcuggccuc cgagaaccag aacaagggca accagacccc cuacgagugg     1980

cugcagggga agaucaacag cgagcgcugg aagaacuucg uggcccucgu ccugggcucc     2040

cagugcagcg cggccaagaa gcagcggcug cucacccagg ugaucgacga caacaaguuc     2100

aucgaccgca accugaacga cacgcgguac aucgcccgcu uccuguccaa cuacauccag     2160

gagaaccucc ugcuggucgg gaagaacaag aagaacgugu ucacccccaa cggccagauc     2220

accgcccucc ugcggagccg cugggggcug aucaaggcgc gggagaacaa caaccgccac     2280

cacgcccucg acgccaucgu ggucgccugc gccacccccu ccaugcagca gaagaucacg     2340

cgguucaucc gcuucaagga ggugcacccg uacaagaucg agaaccggua cgagauggug     2400

gaccaggaga gcggcgagau caucagcccc cacuuccccg agcccugggc guacuuccgc     2460

caggagguca acauccgggu guucgacaac cacccggaca ccgugcugaa ggagaugcug     2520

cccgaccgcc cccaggccaa ccaccaguuc guccagcccc ucuucguguc ccgggccccg     2580

acccgcaaga ugagcggcca ggggcacaug gagaccauca aguccgccaa gcggcuggcc     2640

gagggcauca gcgugcugcg caucccccuc acgcagcuga agcccaaccu gcucgagaac     2700

auggucaaca aggagcggga gccggcgcug uacgccgggc ugaaggcccg ccucgccgag     2760

uucaaccagg accccgccaa ggcguucgcc acccccuucu acaagcaggg cggccagcag     2820

gucaaggcca uccgcgugga gcagguccag aaguccgggg ugcuggugcg ggagaacaac     2880

ggcguggccg acaacgcgag caucgugcgc accgacgugu ucaucaagaa caacaaguuc     2940

uuccuggucc ccaucuacac cuggcagguc gccaagggga uccucccgaa caaggccauc     3000

guggcccaca agaacgagga cgagugggag gagauggacg agggcgccaa guucaaguuc     3060

ucccuguucc ccaacgaccu gguggagcuc aagacgaaga aggaguacuu cuucggguac     3120

uacaucggcc uggaccgggc gaccggcaac aucagccuga aggagcacga cggggagauc     3180

uccaagggca aggacggggu guaccgcgug ggcgucaagc ucgcccugag cuucgagaag     3240

uaccaggugg acgagcuggg gaagaaccgg cagaucugcc gcccccagca gcggcagccc     3300

gugcgcaagc ggccggccgc caccaagaag gccggccagg cgaagaagaa gaaguaagga     3360

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     3420

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     3480

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaagaau u                                               3561


<210>  2372
<211>  3639
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2372
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc ggccgccuuc aagcccaacc ccaucaacua cauccugggc      180

cucgacaucg ggaucgccag cgugggcugg gccauggugg agaucgacga ggacgagaac      240

cccaucugcc ugaucgaccu ggggguccgg guguucgagc gcgcggaggu gccgaagacc      300

ggcgacuccc ucgccauggc ccggcgccug gcccggagcg uccgccggcu gacccgccgg      360

cgcgcgcacc ggcuccugcg cgcccggcgc cugcucaagc gggagggcgu gcugcaggcc      420

gccgacuucg acgagaacgg gcugaucaag ucccucccca acacgcccug gcagcugcgc      480

gccgcggccc uggaccggaa gcucacgccc cuggagugga gcgccgugcu gcuccaccug      540

aucaagcacc gcggcuaccu gucccagcgg aagaacgagg gggagaccgc cgacaaggag      600

cucggcgccc ugcugaaggg ggucgcggac aacgcccacg cccuccagac cggcgacuuc      660

cgcaccccgg ccgagcuggc ccugaacaag uucgagaagg agagcggcca cauccggaac      720

cagcgcgggg acuacuccca caccuucagc cgcaaggacc uccaggcgga gcugauccuc      780

cucuucgaga agcagaagga guucggcaac ccccacgugu ccgggggccu gaaggagggc      840

aucgagaccc ugcucaugac ccagcggccc gcccugagcg gggacgccgu gcagaagaug      900

cugggccacu gcacguucga gcccgccgag ccgaaggccg cgaagaacac cuacaccgcc      960

gagcgcuuca ucuggcucac caagcugaac aaccugcgga uccucgagca gggguccgag     1020

cgcccccuga cggacaccga gcgggccacc cugauggacg agcccuaccg caagagcaag     1080

cucaccuacg cccaggcgcg gaagcugcug ggccucgagg acacggccuu cuucaagggg     1140

cugcgcuacg gcaaggacaa cgccgaggcc uccacccuga uggagaugaa ggccuaccac     1200

gcgaucagcc gggcccucga gaaggagggc cugaaggaca agaagucccc gcugaaccug     1260

agccccgagc uccaggacga gaucgggacc gccuucuccc uguucaagac cgacgaggac     1320

aucacgggcc gccugaagga ccggauccag cccgagaucc ucgaggcccu gcugaagcac     1380

aucagcuucg acaaguucgu ccagaucucc cucaaggccc ugcgccggau cgugccccug     1440

auggagcagg ggaagcgcua cgacgaggcg ugcgccgaga ucuacggcga ccacuacggg     1500

aagaagaaca ccgaggagaa gaucuaccug cccccgaucc ccgccgacga gauccggaac     1560

cccguggucc ugcgcgcccu gagccaggcc cggaagguga ucaacggcgu gguccgccgg     1620

uacggcagcc ccgcgcgcau ccacaucgag accgcccgcg agguggggaa guccuucaag     1680

gaccggaagg agaucgagaa gcgccaggag gagaaccgga aggaccgcga gaaggccgcc     1740

gcgaaguucc gggaguacuu cccgaacuuc gugggcgagc ccaagagcaa ggacauccuc     1800

aagcugcgcc uguacgagca gcagcacggg aagugccucu acuccggcaa ggagaucaac     1860

cuggggcggc ugaacgagaa gggcuacguc gagaucgacc acgcccuccc cuucagccgc     1920

accugggacg acuccuucaa caacaaggug cuggugcugg gcagcgagaa ccagaacaag     1980

gggaaccaga cgcccuacga guacuucaac ggcaaggaca acucccggga guggcaggag     2040

uucaaggccc gcgucgagac cagccgguuc ccgcgcucca agaagcagcg gauccuccug     2100

cagaaguucg acgaggacgg guucaaggag cgcaaccuga acgacacccg guacgugaac     2160

cgcuuccucu gccaguucgu cgccgaccgg augcgccuga ccggcaaggg caagaagcgg     2220

guguucgcca gcaacgggca gaucacgaac cugcuccgcg gcuucugggg gcugcggaag     2280

gugcgcgcgg agaacgaccg ccaccacgcc cuggacgccg ucgugguggc cugcuccacc     2340

gucgccaugc agcagaagau cacccgguuc gugcgcuaca aggagaugaa cgcguucgac     2400

ggcaagacca ucgacaagga gacgggggag gugcuccacc agaagaccca cuucccccag     2460

cccugggagu ucuucgccca ggaggucaug auccgggugu ucggcaagcc ggacggcaag     2520

cccgaguucg aggaggccga cacccccgag aagcugcgca cccugcucgc cgagaagcug     2580

agcucccggc ccgaggccgu gcacgaguac gucacgccgc uguucgugag ccgcgcgccc     2640

aaccggaaga uguccgggca gggccacaug gagaccguca agagcgccaa gcgccucgac     2700

gagggggugu ccgugcugcg ggugccccug acccagcuca agcugaagga ccuggagaag     2760

auggucaacc gcgagcggga gcccaagcuc uacgaggccc ugaaggcccg ccuggaggcg     2820

cacaaggacg acccggccaa ggccuucgcc gagcccuucu acaaguacga caaggccggc     2880

aaccggaccc agcaggucaa ggcggugcgc guggagcagg uccagaagac cggggugugg     2940

gugcggaacc acaacggcau cgccgacaac gccacgaugg ugcgcgugga cgucuucgag     3000

aagggcgaca aguacuaccu cgugcccauc uacagcuggc aggucgccaa ggggauccug     3060

cccgaccggg ccguggugca gggcaaggac gaggaggacu ggcagcugau cgacgacucc     3120

uucaacuuca aguucagccu ccacccgaac gaccuggugg aggucaucac caagaaggcg     3180

cgcauguucg gguacuucgc cagcugccac cggggcaccg ggaacaucaa cauccgcauc     3240

cacgaccugg accacaagau cggcaagaac ggcauccucg aggggaucgg cgugaagacc     3300

gcccuguccu uccagaagua ccagaucgac gagcugggga aggagauccg ccccugccgg     3360

cucaagaagc gcccgcccgu gcggaagcgc cccgccgcca cgaagaaggc gggccaggcc     3420

aagaagaaga aguaaggacu aguaauaagg aaacuguaug aaugucugug ggcaggaagu     3480

gaagagaucc uucuguaaag guuuuuggaa uuaugucugc ugaauaauaa acuuuuuuga     3540

aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            3639


<210>  2373
<211>  3645
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2373
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gaaguaccac gugggcaucg acguggggac cuucagcguc      180

ggccuggccg ccaucgaggu ggacgacgcc gggaugccca ucaagacccu gucccuggug      240

agccacaucc acgacuccgg ccuggacccc gacgagauca agagcgccgu cacgcggcuc      300

gcguccagcg gcaucgcccg ccggacccgc cggcuguacc gccggaagcg ccggcgccug      360

cagcagcucg acaaguucau ccagcggcag ggguggcccg ugaucgagcu ggaggacuac      420

uccgacccgc uguaccccug gaaggugcgc gccgagcucg ccgcgagcua caucgccgac      480

gagaaggagc ggggcgagaa gcuguccguc gcccugcgcc acaucgcccg gcaccgcggg      540

uggcggaacc ccuacgccaa ggugagcucc cucuaccugc ccgacggccc gagcgacgcg      600

uucaaggcca uccgcgagga gaucaagcgg gccuccgggc agcccgugcc cgagaccgcc      660

accgucggcc agauggugac gcugugcgag cucggcaccc ugaagcugcg cggggagggc      720

ggggugcuca gcgcccggcu gcagcagucc gacuacgcgc gcgagaucca ggagaucugc      780

cgcaugcagg agaucggcca ggagcuguac cggaagauca ucgacgucgu guucgccgcc      840

gagagcccca agggcuccgc cagcucccgc guggggaagg acccgcucca gcccggcaag      900

aaccgggccc ugaaggcgag cgacgccuuc cagcgcuacc ggaucgccgc ccugaucggg      960

aaccuccgcg uccgggugga cggcgagaag cgcauccuga gcguggagga gaagaaccug     1020

gucuucgacc accucgugaa ccugaccccc aagaaggagc ccgagugggu gaccaucgcc     1080

gagauccugg ggaucgaccg gggccagcuc aucggcacgg cgaccaugac cgacgacggg     1140

gagcgcgccg gcgcccggcc gcccacccac gacacgaacc gcuccaucgu caacagccgg     1200

aucgcgcccc ugguggacug guggaagacc gccuccgccc uggagcagca cgccaugguc     1260

aaggcccuca gcaacgccga gguggacgac uucgacuccc ccgagggggc gaaggugcag     1320

gccuucuucg ccgaccugga cgacgacguc cacgccaagc uggacagccu ccaccucccg     1380

gugggccgcg ccgcguacuc cgaggacacc cuggugcggc ucacccgccg gaugcugagc     1440

gacggggucg accuguacac ggcccgccuc caggaguucg gcaucgagcc cuccuggacc     1500

ccgcccacgc cccggaucgg cgagcccgug gggaaccccg ccguggaccg cguccugaag     1560

accgugagcc gcuggcugga guccgccacc aagaccuggg gcgcccccga gcgggugauc     1620

aucgagcacg uccgcgaggg guucgugacc gagaagcggg cgcgcgagau ggacggcgac     1680

augcggcgcc gggccgcccg caacgccaag cuguuccagg agaugcagga gaagcugaac     1740

gugcagggga agccgagccg ggcggaccug uggcgcuacc aguccgucca gcggcagaac     1800

ugccagugcg ccuacugcgg cagccccauc accuucucca acagcgagau ggaccacauc     1860

gugccccgcg ccggccaggg guccacgaac acccgggaga accucguggc cgucugccac     1920

cgcugcaacc agagcaaggg caacaccccg uucgccaucu gggcgaagaa caccuccauc     1980

gaggggguga gcgugaagga ggccgucgag cggacgcgcc acugggugac cgacaccggc     2040

augcggagca ccgacuucaa gaaguucacg aaggccgugg ucgagcgcuu ccagcgggcc     2100

accauggacg aggagaucga cgcccgcucc auggagagcg uggcguggau ggccaacgag     2160

cugcgguccc gcguggccca gcacuucgcc agccacggca ccaccguccg cguguaccgg     2220

gggucccuga cggccgaggc gcgccgggcc agcggcaucu ccgggaagcu gaaguucuuc     2280

gacggcgugg gcaagagccg ccucgaccgg cgccaccacg ccaucgacgc cgcggucauc     2340

gccuucaccu ccgacuacgu ggccgagacc cuggccgugc ggagcaaccu gaagcagucc     2400

caggcccacc gccaggaggc gccccagugg cgggaguuca ccgggaagga cgccgagcac     2460

cgcgccgccu ggcgggucug gugccagaag auggagaagc ucagcgcccu gcugaccgag     2520

gaccuccgcg acgaccgggu gguggucaug uccaacgugc gccugcggcu gggcaacggg     2580

agcgcgcaca aggagacgau cggcaagcuc uccaagguga agcugagcuc ccagcugagc     2640

gucagcgaca ucgacaaggc cuccagcgag gcccucuggu gcgcccugac ccgcgagccc     2700

ggguucgacc ccaaggaggg ccugccggcc aaccccgagc ggcacauccg cgugaacggg     2760

acccacgugu acgcgggcga caacaucggc cucuuccccg ucuccgccgg gagcaucgcc     2820

cugcggggcg gguacgccga gcugggcucc agcuuccacc acgcccgcgu guacaagauc     2880

accuccggga agaagcccgc guucgccaug cuccgcgugu acacgaucga ccugcugccg     2940

uaccggaacc aggaccucuu cagcgucgag cugaagcccc agaccauguc caugcgccag     3000

gccgagaaga agcugcggga cgcccucgcg accggcaacg ccgaguaccu gggcuggcug     3060

gugguggacg acgagcucgu cguggacacc agcaagaucg ccacggacca ggugaaggcc     3120

gucgaggccg agcuggggac cauccgccgg uggcgcgugg acggcuucuu cucccccagc     3180

aagcugcggc uccgcccccu gcagaugucc aaggagggga ucaagaagga gagcgcgccg     3240

gagcugucca agaucaucga ccggcccggc uggcuccccg ccgucaacaa gcuguucagc     3300

gacgggaacg ugaccguggu ccgccgggac ucccugggcc gcgugcggcu cgagagcacc     3360

gcccaccucc ccgugacgug gaagguccag aagcgcccgg ccgccaccaa gaaggcgggc     3420

caggccaaga agaagaagua aggacuagua auaaggaaac uguaugaaug ucugugggca     3480

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     3540

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3645


<210>  2374
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2374
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc ggcccggauc cuggccuucg acaucggcau cagcuccauc      180

gggugggccu ucagcgagaa cgacgagcuc aaggacugcg gcgugcgcau cuucaccaag      240

guggagaacc ccaagaccgg ggagucccug gcccugcccc ggcgccucgc gcggagcgcc      300

cgcaagcggc uggcccgccg gaaggcccgc cugaaccacc ucaagcaccu gaucgcgaac      360

gaguucaagc ugaacuacga ggacuaccag uccuucgacg agagccucgc caaggccuac      420

aagggcuccc ugaucagccc cuacgagcug cgguuccgcg cccucaacga gcugcugucc      480

aagcaggacu ucgcccgggu cauccuccac aucgcgaagc gccggggcua cgacgacauc      540

aagaacagcg acgacaagga gaagggggcc auccugaagg ccaucaagca gaacgaggag      600

aagcuggcca acuaccaguc cgugggcgag uaccucuaca aggaguacuu ccagaaguuc      660

aaggagaaca gcaaggaguu cacgaacgug cgcaacaaga aggaguccua cgagcggugc      720

aucgcccaga gcuuccugaa ggacgagcug aagcucaucu ucaagaagca gcgcgaguuc      780

ggguucuccu ucagcaagaa guucgaggag gagguccugu ccguggcguu cuacaagcgg      840

gcccugaagg acuucagcca ccucgugggc aacugcuccu ucuucaccga cgagaagcgc      900

gccccgaaga acagcccccu ggccuucaug uucgucgccc ugacccggau caucaaccuc      960

cugaacaacc ugaagaacac cgaggggauc cucuacacga aggacgaccu gaacgcgcug     1020

cucaacgagg ugcugaagaa cggcacccug accuacaagc agaccaagaa gcuccugggc     1080

cugagcgacg acuacgaguu caagggggag aagggcacgu acuucaucga guucaagaag     1140

uacaaggagu ucaucaaggc ccucggggag cacaaccugu cccaggacga ccugaacgag     1200

aucgccaagg acaucacccu caucaaggac gagaucaagc ugaagaaggc ccuggccaag     1260

uacgaccuca accagaacca gaucgacagc cuguccaagc uggaguucaa ggaccaccuc     1320

aacaucagcu ucaaggcgcu gaagcuggug acgccccuga ugcucgaggg caagaaguac     1380

gacgaggccu gcaacgagcu gaaccugaag gucgccauca acgaggacaa gaaggacuuc     1440

cuccccgccu ucaacgagac cuacuacaag gacgagguga ccaacccggu gguccugcgc     1500

gcgaucaagg aguaccgcaa ggugcugaac gcccuccuga agaaguacgg caaggugcac     1560

aagaucaaca ucgagcuggc ccgggagguc gggaagaacc acucccagcg cgccaagauc     1620

gagaaggagc agaacgagaa cuacaaggcc aagaaggacg cggagcucga gugcgagaag     1680

cugggccuga agaucaacag caagaacauc cucaagcugc ggcuguucaa ggagcagaag     1740

gaguucugcg ccuacuccgg ggagaagauc aagaucagcg accuccagga cgagaagaug     1800

cuggagaucg accacaucua ccccuacucc cgcagcuucg acgacuccua caugaacaag     1860

gugcuggugu ucaccaagca gaaccaggag aagcucaacc agacccccuu cgaggccuuc     1920

ggcaacgaca gcgccaagug gcagaagauc gagguccugg ccaagaaccu gcccaccaag     1980

aagcagaagc ggauccucga caagaacuac aaggacaagg agcagaagaa cuucaaggac     2040

cgcaaccuga acgacacgcg guacaucgcg cgccuggugc ucaacuacac caaggacuac     2100

cuggacuucc ugccgcucuc cgacgacgag aacaccaagc ugaacgacac ccagaagggg     2160

agcaaggugc acgucgaggc caaguccggc augcugacga gcgcccuccg gcacaccugg     2220

ggcuucuccg ccaaggaccg caacaaccac cuccaccacg ccaucgacgc ggugaucauc     2280

gccuacgcca acaacagcau cgucaaggcc uucuccgacu ucaagaagga gcaggagagc     2340

aacagcgcgg agcuguacgc caagaagauc uccgagcugg acuacaagaa caagcggaag     2400

uucuucgagc ccuucagcgg guuccgccag aaggugcugg acaagaucga cgagaucuuc     2460

guguccaagc ccgagcggaa gaagcccagc ggcgcccugc acgaggagac cuuccgcaag     2520

gaggaggagu ucuaccaguc cuacgggggc aaggaggggg uccucaaggc ccuggagcug     2580

ggcaagaucc ggaaggugaa cggcaagauc gugaagaacg gggacauguu ccgcgucgac     2640

aucuucaagc acaagaagac caacaaguuc uacgccgugc cgaucuacac gauggacuuc     2700

gcgcucaagg ugcugcccaa caaggccguc gcccggagca agaagggcga gaucaaggac     2760

uggauccuga uggacgagaa cuacgaguuc ugcuucuccc ucuacaagga cagccugauc     2820

cugauccaga ccaaggacau gcaggagccc gaguucgugu acuacaacgc cuucaccucc     2880

agcaccgugu cccucaucgu cagcaagcac gacaacaagu ucgagacgcu guccaagaac     2940

cagaagaucc uguucaagaa cgccaacgag aaggagguga ucgccaagag caucgggauc     3000

cagaaccuca agguguucga gaaguacauc gucuccgcgc ugggcgaggu gaccaaggcc     3060

gaguuccgcc agcgggagga cuucaagaag aagcgccccg ccgccaccaa gaaggcgggg     3120

caggccaaga agaagaagua aggacuagua auaaggaaac uguaugaaug ucugugggca     3180

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     3240

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3300

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3345


<210>  2375
<211>  3756
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2375
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gagcgaccug gugcucggcc uggacaucgg gaucggcucc      180

gugggggucg gcauccugaa caaggugacc ggcgagauca uccacaagaa cagccggauc      240

uuccccgccg cccaggccga gaacaaccuc gugcgccgga ccaaccgcca ggggcggcgc      300

cuggcccggc gcaagaagca ccggcgcguc cggcugaacc gccucuucga ggaguccggc      360

cugaucacgg acuucaccaa gaucagcauc aaccugaacc ccuaccagcu ccgggugaag      420

gggcugaccg acgagcuguc caacgaggag cuguucaucg cgcugaagaa cauggugaag      480

caccgcggca ucagcuaccu ggacgacgcc uccgacgacg ggaacagcuc cgucggcgac      540

uacgcccaga ucgugaagga gaacagcaag cagcucgaga ccaagacgcc cggccagauc      600

cagcuggagc gguaccagac cuacgggcag cugcgcggcg acuucaccgu ggagaaggac      660

gggaagaagc accggcucau caacgucuuc ccgaccuccg ccuaccgcag cgaggcgcug      720

cggauccugc agacgcagca ggaguucaac ccccagauca ccgacgaguu caucaaccgc      780

uaccucgaga uccugaccgg caagcggaag uacuaccacg gccccgggaa cgagaagucc      840

cgcaccgacu acggccgcua ccggacgagc ggggagaccc uggacaacau cuucggcauc      900

cucaucggga agugcaccuu cuaccccgac gaguuccgcg ccgccaaggc cuccuacacc      960

gcccaggagu ucaaccugcu gaacgaccuc aacaaccuga cggugccgac cgagaccaag     1020

aagcugagca aggagcagaa gaaccagauc aucaacuacg ugaagaacga gaaggcgaug     1080

ggccccgcca agcuguucaa guacaucgcc aagcuccugu ccugcgacgu cgccgacauc     1140

aagggcuacc ggaucgacaa gagcgggaag gccgagaucc acaccuucga ggcguaccgc     1200

aagaugaaga cgcucgagac ccuggacauc gagcagaugg accgggagac ccuggacaag     1260

cucgccuacg ugcugacccu gaacacggag cgcgagggca uccaggaggc ccucgagcac     1320

gaguucgccg acggguccuu cagccagaag caggucgacg agcuggugca guuccggaag     1380

gccaacagcu ccaucuucgg caaggggugg cacaacuuca gcgugaagcu gaugauggag     1440

cucauccccg agcuguacga gaccuccgag gagcagauga ccauccugac ccgccucggc     1500

aagcagaaga cgaccagcuc cagcaacaag accaaguaca ucgacgagaa gcugcugacc     1560

gaggagaucu acaaccccgu ggucgcgaag uccgugcggc aggccaucaa gaucgugaac     1620

gccgccauca aggaguacgg cgacuucgac aacaucguca ucgagauggc ccgcgagacg     1680

aacgaggacg acgagaagaa ggcgauccag aagauccaga aggccaacaa ggacgagaag     1740

gacgccgcca ugcucaaggc ggccaaccag uacaacggga aggccgagcu gccgcacagc     1800

guguuccacg gccacaagca gcuggccacc aagauccggc uguggcacca gcagggggag     1860

cgcugccucu acaccggcaa gaccaucucc auccacgacc ugaucaacaa cagcaaccag     1920

uucgaggucg accacauccu gccccucucc aucacguucg acgacagccu ggccaacaag     1980

gugcuggugu acgcgaccgc caaccaggag aaggggcagc ggacccccua ccaggcccuc     2040

gacuccaugg acgacgccug gagcuuccgc gagcugaagg ccuucguccg ggaguccaag     2100

acccugagca acaagaagaa ggaguaccuc cugaccgagg aggacaucuc caaguucgac     2160

gugcgcaaga aguucaucga gcggaaccug guggacacgc gcuacgcgag ccgggucgug     2220

cucaacgccc ugcaggagca cuuccgcgcc cacaagaucg acaccaaggu guccgucgug     2280

cgcggccagu ucaccagcca gcugcggcgc cacuggggca ucgagaagac ccgggacacg     2340

uaccaccacc acgccgugga cgcccucauc aucgcggcca gcucccagcu gaaccugugg     2400

aagaagcaga agaacacccu cgucagcuac uccgaggacc agcugcugga caucgagacc     2460

ggggagcuca ucagcgacga cgaguacaag gaguccgugu ucaaggcccc cuaccagcac     2520

uucguggaca cccucaagag caaggaguuc gaggacucca uccuguucag cuaccagguc     2580

gacuccaagu ucaaccgcaa gaucagcgac gccacgaucu acgcgacccg gcaggccaag     2640

gugggcaagg acaaggccga cgagaccuac gugcucggga agaucaagga caucuacacc     2700

caggacggcu acgacgccuu caugaagauc uacaagaagg acaaguccaa guuccugaug     2760

uaccgccacg acccgcagac guucgagaag gucaucgagc ccauccugga gaacuacccc     2820

aacaagcaga ucaacgagaa gggcaaggag gugcccugca acccguuccu caaguacaag     2880

gaggagcacg gguacauccg gaaguacagc aagaagggca acgggcccga gaucaagucc     2940

cugaaguacu acgacagcaa gcugggcaac cacaucgaca ucacccccaa ggacuccaac     3000

aacaaggugg uccuccagag cguguccccc uggcgcgccg acguguacuu caacaagacc     3060

accgggaagu acgagauccu gggccugaag uacgcggacc uccaguucga gaagggcacg     3120

gggaccuaca agaucagcca ggagaaguac aacgacauca agaagaagga gggcgucgac     3180

agcgacuccg aguucaaguu cacccuguac aagaacgacc ugcuccuggu gaaggacacc     3240

gagacgaagg agcagcagcu guuccgguuc cucagccgca ccaugccgaa gcagaagcac     3300

uacguggagc ugaagcccua cgacaagcag aaguucgagg gcggggaggc ccugaucaag     3360

guccucggga acguggccaa cuccggccag ugcaagaagg gccugggcaa gagcaacauc     3420

uccaucuaca aggugcggac cgacguccug gggaaccagc acaucaucaa gaacgagggg     3480

gacaagccca agcucgacuu caagcgcccc gccgccacca agaaggcggg ccaggccaag     3540

aagaagaagu aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     3600

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     3660

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3756


<210>  2376
<211>  4314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2376
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gacccaguuc gagggcuuca ccaaccugua ccaggugagc      180

aagacgcucc gguucgagcu gaucccccag gggaagaccc ugaagcacau ccaggagcag      240

ggcuucaucg aggaggacaa ggcccgcaac gaccacuaca aggagcucaa gcccaucauc      300

gaccggaucu acaagaccua cgccgaccag ugccugcagc uggugcagcu cgacugggag      360

aaccuguccg ccgccaucga cagcuaccgc aaggagaaga ccgaggagac gcggaacgcg      420

cugaucgagg agcaggccac cuaccgcaac gccauccacg acuacuucau cgggcggacc      480

gacaaccuca ccgacgccau caacaagcgc cacgcggaga ucuacaaggg ccuguucaag      540

gccgagcugu ucaacggcaa gguccucaag cagcugggga cggugaccac caccgagcac      600

gagaacgccc ugcuccgguc cuucgacaag uucacgaccu acuucagcgg cuucuacgag      660

aaccgcaaga acguguucuc cgccgaggac aucagcaccg ccauccccca ccggaucguc      720

caggacaacu ucccgaaguu caaggagaac ugccacaucu ucacccgccu gaucacggcg      780

gugcccuccc ugcgggagca cuucgagaac gugaagaagg ccaucgggau cuucgucagc      840

accuccaucg aggagguguu cagcuucccc uucuacaacc agcuccugac ccagacccag      900

aucgaccugu acaaccagcu ccugggcggg aucucccgcg aggccggcac ggagaagauc      960

aagggccuga acgaggugcu caaccuggcc auccagaaga acgacgagac cgcccacauc     1020

aucgcgagcc ugccccaccg guucaucccc cucuucaagc agauccuguc cgaccgcaac     1080

acccugagcu ucauccucga ggaguucaag uccgacgagg aggucaucca gagcuucugc     1140

aaguacaaga cccugcugcg gaacgagaac gugcucgaga cggccgaggc ccuguucaac     1200

gagcugaacu ccaucgaccu cacccacauc uucaucagcc acaagaagcu ggagaccauc     1260

uccagcgccc ugugcgacca cugggacacc cuccgcaacg cccuguacga gcggcgcauc     1320

agcgagcuga cggggaagau caccaagucc gcgaaggaga aggugcagcg gagccucaag     1380

cacgaggaca ucaaccugca ggagaucauc uccgccgccg gcaaggagcu gagcgaggcc     1440

uucaagcaga agaccuccga gauccucagc cacgcccacg cggcccugga ccagccgcug     1500

cccaccacgc ucaagaagca ggaggagaag gagauccuga agucccagcu ggacagccug     1560

cucgggcugu accaccuccu cgacugguuc gccgucgacg aguccaacga gguggacccc     1620

gaguucagcg cccgccugac cggcaucaag cuggagaugg agccgucccu cagcuucuac     1680

aacaaggcgc gcaacuacgc caccaagaag cccuacuccg uggagaaguu caagcugaac     1740

uuccagaugc ccacccuggc cagcggcugg gacgucaaca aggagaagaa caacggggcc     1800

auccucuucg ugaagaacgg ccuguacuac cuggggauca ugcccaagca gaagggccgg     1860

uacaaggccc ucuccuucga gccgacggag aagaccagcg agggguucga caagauguac     1920

uacgacuacu uccccgacgc ggccaagaug auccccaagu gcuccaccca gcugaaggcc     1980

gugaccgccc acuuccagac ccacacgacc cccauccugc ucagcaacaa cuucaucgag     2040

ccgcuggaga ucaccaagga gaucuacgac cugaacaacc ccgagaagga gcccaagaag     2100

uuccagaccg ccuacgcgaa gaagacgggc gaccagaagg gcuaccgcga ggcccucugc     2160

aaguggaucg acuucacccg ggacuuccug uccaaguaca ccaagaccac gagcaucgac     2220

cugagcuccc uccgccccag cucccaguac aaggaccugg gggaguacua cgccgagcug     2280

aacccgcucc uguaccacau cagcuuccag cggaucgccg agaaggagau cauggacgcc     2340

gucgagaccg gcaagcugua ccucuuccag aucuacaaca aggacuucgc gaaggggcac     2400

cacggcaagc ccaaccugca cacccuguac uggaccggcc ucuucucccc cgagaaccug     2460

gccaagacga gcaucaagcu gaacgggcag gccgagcugu ucuaccgccc caagucccgg     2520

augaagcgca uggcccaccg gcugggcgag aagaugcuga acaagaagcu caaggaccag     2580

aagaccccga uccccgacac ccuguaccag gagcuguacg acuacgugaa ccaccgccuc     2640

agccacgacc uguccgacga ggcgcgggcc cugcucccca acgugaucac caaggagguc     2700

agccacgaga ucaucaagga ccgccgguuc acguccgaca aguucuucuu ccacgugccg     2760

aucacccuga acuaccaggc cgccaacagc cccuccaagu ucaaccagcg cgucaacgcc     2820

uaccugaagg agcaccccga gacccccauc aucgggaucg accggggcga gcgcaaccuc     2880

aucuacauca ccgugaucga cagcacgggg aagauccugg agcagcgguc ccugaacacc     2940

auccagcagu ucgacuacca gaagaagcuc gacaaccgcg agaaggagcg gguggcggcc     3000

cgccaggccu ggagcgucgu gggcaccauc aaggaccuga agcaggggua ccugucccag     3060

gugauccacg agaucgucga ccucaugauc cacuaccagg ccgugguggu ccuggagaac     3120

cugaacuucg gcuucaagag caagcgcacc ggcaucgccg agaaggcggu guaccagcag     3180

uucgagaaga ugcucaucga caagcugaac ugccuggugc ucaaggacua cccggccgag     3240

aaggucgggg gcgugcugaa ccccuaccag cugacggacc aguucaccag cuucgccaag     3300

auggggaccc aguccggcuu ccucuucuac gugcccgccc ccuacaccag caagaucgac     3360

ccgcugacgg gcuucgucga ccccuucgug uggaagacca ucaagaacca cgagucccgg     3420

aagcacuucc uggagggguu cgacuuccuc cacuacgacg ugaagaccgg cgacuucauc     3480

cugcacuuca agaugaaccg caaccugagc uuccagcggg ggcuccccgg cuucaugccc     3540

gccugggaca ucgucuucga gaagaacgag acccaguucg acgcgaaggg gacgccguuc     3600

aucgccggca agcgcaucgu gcccgugauc gagaaccacc gguucaccgg ccgcuaccgg     3660

gaccuguacc ccgccaacga gcugaucgcc cuccuggagg agaaggggau cgucuuccgc     3720

gacggcucca acauccugcc caagcuccug gagaacgacg acagccacgc gaucgacacc     3780

augguggccc ugauccgguc cgugcuccag augcgcaaca gcaacgccgc caccggggag     3840

gacuacauca acuccccggu ccgggaccug aacggcgugu gcuucgacag ccgcuuccag     3900

aaccccgagu ggcccaugga cgccgacgcg aacggggccu accacaucgc ccugaagggc     3960

cagcuccugc ugaaccaccu caaggagucc aaggaccuga agcugcagaa cggcaucagc     4020

aaccaggacu ggcucgccua cauccaggag cugcggaaca agcgccccgc cgcgacgaag     4080

aaggccgggc aggccaagaa gaagaaguaa ggacuaguaa uaaggaaacu guaugaaugu     4140

cugugggcag gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau     4200

aauaaacuuu uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           4314


<210>  2377
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2377
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaagaagcg caaggugggc      120

auccacgggg ucccggccgc gagcaucuac caggaguucg ugaacaagua cucccugagc      180

aagacccucc gguucgagcu gaucccccag ggcaagaccc uggagaacau caaggcccgc      240

gggcucaucc uggacgacga gaagcgggcc aaggacuaca agaaggccaa gcagaucauc      300

gacaaguacc accaguucuu caucgaggag auccugucca gcgugugcau cuccgaggac      360

cuccugcaga acuacagcga cgucuacuuc aagcugaaga aguccgacga cgacaaccuc      420

cagaaggacu ucaagagcgc caaggacacg aucaagaagc agaucuccga guacaucaag      480

gacagcgaga aguucaagaa ccuguucaac cagaaccuga ucgacgcgaa gaagggccag      540

gaguccgacc ucauccugug gcugaagcag agcaaggaca acgggaucga gcuguucaag      600

gccaacuccg acaucaccga caucgacgag gcccucgaga ucaucaagag cuucaagggc      660

uggaccaccu acuucaaggg cuuccacgag aaccgcaaga acguguacuc cagcaacgac      720

auccccacgu ccaucaucua ccggaucgug gacgacaacc ugcccaaguu ccucgagaac      780

aaggccaagu acgagagccu gaaggacaag gcgccggagg ccaucaacua cgagcagauc      840

aagaaggacc uggccgagga gcucaccuuc gacaucgacu acaagaccuc cgaggucaac      900

cagcgcgugu ucagccugga cgagguguuc gagaucgcca acuucaacaa cuaccugaac      960

cagagcggga ucaccaaguu caacacgauc aucggcggga aguucgucaa cggcgagaac     1020

accaagcgga aggggaucaa cgaguacauc aaccucuacu cccagcagau caacgacaag     1080

acccugaaga aguacaagau gagcgugcug uucaagcaga uccucuccga caccgagagc     1140

aaguccuucg ugaucgacaa gcuggaggac gacagcgacg ucgugacgac caugcagucc     1200

uucuacgagc agaucgccgc guucaagacc guggaggaga aguccaucaa ggagacccug     1260

ucccuccugu ucgacgaccu gaaggcccag aagcucgacc ugagcaagau cuacuucaag     1320

aacgacaagu cccugacgga ccucagccag caggucuucg acgacuacuc cgugaucggc     1380

accgccgugc uggaguacau cacccagcag aucgccccca agaaccugga caaccccagc     1440

aagaaggagc aggagcucau cgccaagaag accgagaagg cgaaguaccu gucccuggag     1500

acgaucaagc ucgcccugga ggaguucaac aagcaccgcg acaucgacaa gcagugccgg     1560

uucgaggaga uccuggccaa cuucgccgcc auccccauga ucuucgacga gaucgcgcag     1620

aacaaggaca accucgccca gaucagcauc aaguaccaga accagggcaa gaaggaccug     1680

cugcaggccu ccgccgagga cgacgucaag gccaucaagg accuccugga ccagaccaac     1740

aaccugcugc acaagcucaa gaucuuccac aucagccagu ccgaggacaa ggcgaacauc     1800

cuggacaagg acgagcacuu cuaccuggug uucgaggagu gcuacuucga gcucgccaac     1860

aucgugccgc uguacaacaa gauccgcaac uacaucaccc agaagcccua cagcgacgag     1920

aaguucaagc ugaacuucga gaacagcacc cucgccaacg ggugggacaa gaacaaggag     1980

cccgacaaca cggccauccu guucaucaag gacgacaagu acuaccuggg cgucaugaac     2040

aagaagaaca acaagaucuu cgacgacaag gcgaucaagg agaacaaggg ggagggcuac     2100

aagaagaucg uguacaagcu ccugcccggc gccaacaaga ugcugccgaa gguguucuuc     2160

uccgccaaga gcaucaaguu cuacaacccc uccgaggaca uccuccggau ccgcaaccac     2220

agcacccaca ccaagaacgg gagcccccag aagggcuacg agaaguucga guucaacauc     2280

gaggacugcc ggaaguucau cgacuucuac aagcagagca ucuccaagca ccccgagugg     2340

aaggacuucg gguuccgcuu cagcgacacc cagcgguaca acuccaucga cgaguucuac     2400

cgcgaggucg agaaccaggg cuacaagcug acguucgaga acaucagcga guccuacauc     2460

gacagcgugg ucaaccaggg gaagcuguac cucuuccaga ucuacaacaa ggacuucucc     2520

gccuacagca agggccggcc gaaccugcac acccuguacu ggaaggcccu cuucgacgag     2580

cgcaaccugc aggacguggu guacaagcug aacggcgagg cggagcuguu cuaccggaag     2640

caguccaucc ccaagaagau cacccacccc gccaaggagg ccaucgccaa caagaacaag     2700

gacaacccca agaaggagag cgucuucgag uacgaccuca ucaaggacaa gcgcuucacc     2760

gaggacaagu ucuucuucca cugcccgauc acgaucaacu ucaaguccag cggggccaac     2820

aaguucaacg acgagaucaa ccugcuccug aaggagaagg cgaacgacgu gcacauccug     2880

agcaucgacc ggggcgagcg ccaccucgcc uacuacaccc ugguggacgg gaagggcaac     2940

aucaucaagc aggacaccuu caacaucauc gggaacgacc gcaugaagac caacuaccac     3000

gacaagcugg ccgccaucga gaaggaccgg gacuccgccc gcaaggacug gaagaagauc     3060

aacaacauca aggagaugaa ggagggcuac cucagccagg ucgugcacga gaucgcgaag     3120

cuggugaucg aguacaacgc caucgucgug uucgaggacc ugaacuucgg cuucaagcgg     3180

gggcgcuuca agguggagaa gcaggucuac cagaagcucg agaagaugcu gaucgagaag     3240

cugaacuacc ucguguucaa ggacaacgag uucgacaaga cgggcggggu gcugcgggcc     3300

uaccagcuga ccgcccccuu cgagaccuuc aagaagaugg gcaagcagac cgggaucauc     3360

uacuacgucc ccgcgggcuu caccuccaag aucugccccg ugacgggcuu cgugaaccag     3420

cucuacccga aguacgagag cgucuccaag agccaggagu ucuucuccaa guucgacaag     3480

aucugcuaca accuggacaa gggguacuuc gaguucagcu ucgacuacaa gaacuucggc     3540

gacaaggccg ccaaggggaa guggaccauc gccuccuucg gcagccgccu gaucaacuuc     3600

cggaacuccg acaagaacca caacugggac acccgcgagg uguaccccac caaggagcuc     3660

gagaagcugc ugaaggacua cagcaucgag uacggccacg gggagugcau caaggccgcg     3720

aucugcggcg aguccgacaa gaaguucuuc gccaagcuca cgagcgugcu gaacaccauc     3780

cugcagaugc ggaacuccaa gaccgggacc gagcucgacu accugaucag ccccgucgcc     3840

gacgugaacg gcaacuucuu cgacucccgc caggccccca agaacaugcc gcaggacgcc     3900

gacgcgaacg gggccuacca caucggccug aagggccuca ugcugcuggg gcggaucaag     3960

aacaaccagg agggcaagaa gcucaaccug gugaucaaga acgaggagua cuucgaguuc     4020

guccagaacc gcaacaacaa gcggcccgcc gccacgaaga aggccgggca ggcgaagaag     4080

aagaaguaag gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag     4140

auccuucugu aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau     4200

aaaucuggua gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  4293


<210>  2378
<211>  4439
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2378
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcggac aagaaguaca gcaucggccu ggacaucggg      180

accaacuccg ugggcugggc cgugaucacc gacgaguaca agguccccag caagaaguuc      240

aaggugcugg ggaacacgga ccggcacucc aucaagaaga accugaucgg cgcccugcug      300

uucgacagcg gcgagaccgc cgaggccacc cgccugaagc ggaccgcgcg ccggcgcuac      360

acgcggcgca agaaccggau cugcuaccug caggagaucu ucuccaacga gauggccaag      420

guggacgaca gcuucuucca ccgccucgag gaguccuucc uggucgagga ggacaagaag      480

cacgagcggc accccaucuu cgggaacauc guggacgagg uggccuacca cgagaaguac      540

cccaccaucu accaccuccg caagaagcuc gucgacagca ccgacaaggc cgaccugcgg      600

cugaucuacc ucgcgcuggc ccacaugauc aaguuccgcg gccacuuccu gaucgagggg      660

gaccucaacc cggacaacuc cgacguggac aagcuguuca uccagcuggu gcagaccuac      720

aaccagcugu ucgaggagaa ccccaucaac gccagcggcg ucgacgccaa ggccauccug      780

uccgcgcggc ugagcaaguc ccgccggcuc gagaaccuga ucgcccagcu gcccggggag      840

aagaagaacg gccucuucgg caaccugauc gcccugagcc ucgggcugac gcccaacuuc      900

aaguccaacu ucgaccuggc cgaggacgcc aagcuccagc ugagcaagga caccuacgac      960

gacgaccugg acaaccuccu ggcgcagauc ggcgaccagu acgccgaccu guuccucgcc     1020

gccaagaacc uguccgacgc cauccugcuc agcgacaucc ugcgcgugaa caccgagauc     1080

accaaggcgc cgcuguccgc cagcaugauc aagcgguacg acgagcacca ccaggaccuc     1140

acgcugcuga aggcccucgu gcgccagcag cugcccgaga aguacaagga gaucuucuuc     1200

gaccagagca agaacgggua cgccggcuac aucgacggcg gggccuccca ggaggaguuc     1260

uacaaguuca ucaagcccau ccuggagaag auggacggca ccgaggagcu ccuggucaag     1320

cugaaccggg aggaccugcu ccgcaagcag cgcaccuucg acaacgggag caucccccac     1380

cagauccacc ugggcgagcu gcacgcgauc cuccggcgcc aggaggacuu cuacccguuc     1440

cugaaggaca accgggagaa gaucgagaag auccugaccu uccgcauccc cuacuacgug     1500

gggccccucg cccggggcaa cucccgcuuc gccuggauga cgcgcaagag cgaggagacc     1560

aucacccccu ggaacuucga ggaggugguc gacaagggcg ccuccgcgca gagcuucauc     1620

gagcggauga ccaacuucga caagaaccug ccgaacgaga aggugcugcc caagcacucc     1680

cuccuguacg aguacuucac gguguacaac gagcugacca aggucaagua cgugaccgag     1740

gggaugcgga agcccgccuu ccucagcggc gagcagaaga aggccaucgu ggaccugcug     1800

uucaagacca accgcaaggu cacggugaag cagcucaagg aggacuacuu caagaagauc     1860

gagugcuucg acuccgucga gaucagcggg guggaggacc gguucaacgc cucccugggc     1920

accuaccacg accugcucaa gaucaucaag gacaaggacu uccuggacaa cgaggagaac     1980

gaggacaucc uggaggacau cgugcucacc cugacccugu ucgaggaccg cgagaugauc     2040

gaggagcggc ucaagacgua cgcccaccug uucgacgaca aggucaugaa gcagcugaag     2100

cgccggcgcu acaccgggug gggccggcuc agccgcaagc ugaucaacgg cauccgggac     2160

aagcaguccg ggaagaccau ccuggacuuc cucaagagcg acggcuucgc gaaccgcaac     2220

uucaugcagc ugauccacga cgacucccug accuucaagg aggacaucca gaaggcccag     2280

gugagcgggc agggcgacuc ccuccacgag cacaucgcca accuggccgg gagccccgcc     2340

aucaagaagg gcauccugca gacggugaag gucguggacg agcucgugaa ggucaugggc     2400

cgccacaagc cggagaacau cgugaucgag auggcgcggg agaaccagac cacccagaag     2460

gggcagaaga acucccgcga gcggaugaag cgcaucgagg agggcaucaa ggagcugggg     2520

agccagaucc ugaaggagca ccccguggag aacacccagc uccagaacga gaagcuguac     2580

cuguacuacc uccagaacgg ccgggacaug uacgucgacc aggagcugga caucaaccgc     2640

cugagcgacu acgacgugga ccacaucgug ccccaguccu uccucaagga cgacagcauc     2700

gacaacaagg uccugacgcg guccgacaag aaccgcggca agagcgacaa cgugcccucc     2760

gaggaggugg ucaagaagau gaagaacuac uggcggcagc ugcucaacgc caagcugauc     2820

acccagcgca aguucgacaa ccugaccaag gccgagcgcg ggggccucag cgagcuggac     2880

aaggccgggu ucaucaagcg gcagcuggug gagacccggc agaucaccaa gcacguggcc     2940

cagauccucg acucccgcau gaacacgaag uacgacgaga acgacaagcu gauccgggag     3000

gucaagguga ucacccucaa gagcaagcuc guguccgacu uccgcaagga cuuccaguuc     3060

uacaaggucc gggagaucaa caacuaccac cacgcgcacg acgccuaccu gaacgccgug     3120

gugggcaccg cccugaucaa gaaguacccg aagcucgaga gcgaguucgu cuacggggac     3180

uacaaggugu acgacgugcg caagaugauc gcgaaguccg agcaggagau cggcaaggcc     3240

accgccaagu acuucuucua cagcaacauc augaacuucu ucaagacgga gaucacccug     3300

gccaacggcg agauccggaa gcgcccccug aucgagacca acggggagac cggcgagauc     3360

gucugggaca aggggcggga cuucgccacg gugcgcaagg ugcucuccau gccccagguc     3420

aacaucguga agaagaccga ggugcagacc ggcggguuca gcaaggaguc cauccugccc     3480

aagcgcaaca gcgacaagcu gaucgcgcgg aagaaggacu gggacccgaa gaaguacggc     3540

ggcuucgacu cccccaccgu cgccuacagc gugcucgugg ucgccaaggu ggagaagggc     3600

aagagcaaga agcugaaguc cgugaaggag cugcucggga ucacgaucau ggagcgcagc     3660

uccuucgaga agaaccccau cgacuuccug gaggccaagg gguacaagga ggucaagaag     3720

gaccugauca ucaagcuccc gaaguacagc cuguucgagc uggagaacgg ccggaagcgc     3780

augcucgccu ccgcggggga gcugcagaag ggcaacgagc uggcccuccc cagcaaguac     3840

gugaacuucc uguaccuggc cucccacuac gagaagcuca agggcagccc cgaggacaac     3900

gagcagaagc agcuguucgu ggagcagcac aagcacuacc uggacgagau caucgagcag     3960

aucuccgagu ucagcaagcg ggucauccuc gccgacgcca accuggacaa ggugcugucc     4020

gcguacaaca agcaccgcga caagcccauc cgggagcagg ccgagaacau cauccaccuc     4080

uucacccuga ccaaccuggg ggccccggcc gcguucaagu acuucgacac cacgaucgac     4140

cgcaagcggu acaccagcac caaggaggug cucgacgcca cccugaucca ccaguccauc     4200

acgggccugu acgagacccg caucgaccuc agccagcucg ggggcgacaa gcggcccgcc     4260

gccaccaaga aggccgggca ggcgaagaag aagaaguaag gacuaguccc uguucccaga     4320

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      4439


<210>  2379
<211>  5222
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2379
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaac uucaagaucc ugcccaucgc caucgaccuc      180

ggcgugaaga acaccggggu guucagcgcc uucuaccaga agggcaccuc ccuggagcgg      240

cuggacaaca agaacgggaa ggucuacgag cucagcaagg acuccuacac gcugcugaug      300

aacaaccgca ccgcccggcg ccaccagcgg cgcggcaucg accggaagca gcucgugaag      360

cgccuguuca agcugaucug gaccgagcag cucaaccugg agugggacaa ggacacccag      420

caggccauca gcuuccuguu caaccggcgc ggcuucuccu ucaucacgga cggguacagc      480

cccgaguacc ucaacaucgu gcccgagcag gucaaggcga uccugaugga caucuucgac      540

gacuacaacg gcgaggacga ccuggacucc uaccucaagc uggccaccga gcaggagagc      600

aagaucuccg agaucuacaa caagcugaug cagaagaucc ucgaguucaa gcugaugaag      660

cugugcaccg acaucaagga cgacaaggug agcaccaaga cgcucaagga gaucaccucc      720

uacgaguucg agcugcuggc cgacuaccuc gccaacuaca gcgagucccu gaagacccag      780

aaguucagcu acaccgacaa gcaggggaac cugaaggagc ucuccuacua ccaccacgac      840

aaguacaaca uccaggaguu ccugaagcgg cacgcgacga ucaacgaccg cauccuggac      900

acccuccuga ccgacgaccu ggacaucugg aacuucaacu ucgagaaguu cgacuucgac      960

aagaacgagg agaagcucca gaaccaggag gacaaggacc acauccaggc ccaccuccac     1020

cacuucgugu ucgccgucaa caagaucaag agcgagaugg ccuccggcgg gcggcaccgc     1080

agccaguacu uccaggagau caccaacgug cuggacgaga acaaccacca ggagggcuac     1140

cugaagaacu ucugcgagaa ccugcacaac aagaaguacu ccaaccugag cgugaagaac     1200

cucgucaacc ugaucggcaa ccugagcaac cucgagcuga agccgcugcg gaaguacuuc     1260

aacgacaaga uccacgccaa ggcggaccac ugggacgagc agaaguucac ggagaccuac     1320

ugccacugga uccucgggga guggcgcgug ggcgugaagg accaggacaa gaaggacggg     1380

gccaaguacu ccuacaagga ccugugcaac gagcugaagc agaaggucac caaggccggc     1440

cucguggacu uccugcugga gcuggacccc ugccggacca ucccgcccua ccucgacaac     1500

aacaaccgca agccgcccaa gugccagagc cugauccuga accccaaguu ccucgacaac     1560

caguacccca acuggcagca guaccugcag gagcugaaga agcuccaguc cauccagaac     1620

uaccuggaca gcuucgagac ggaccugaag gugcucaagu ccagcaagga ccagcccuac     1680

uucgucgagu acaaguccag caaccagcag aucgccuccg gccagcggga cuacaaggac     1740

cuggacgccc gcauccugca guucaucuuc gaccggguga aggcgagcga cgagcuccug     1800

cugaacgaga ucuacuucca ggccaagaag cucaagcaga aggccuccag cgagcuggag     1860

aagcuggagu ccagcaagaa gcucgacgag gugaucgcca acucccagcu gagccagauc     1920

cugaaguccc agcacaccaa cgggaucuuc gagcagggca ccuuccucca ccuggucugc     1980

aaguacuaca agcagcgcca gcgcgcccgg gacagccgcc uguacaucau gcccgaguac     2040

cgguacgaca agaagcucca caaguacaac aacaccgggc gcuucgacga cgacaaccag     2100

cugcugacgu acugcaacca caagccccgg cagaagcgcu accagcuccu gaacgaccug     2160

gcgggcgugc uccagguguc ccccaacuuc cugaaggaca agaucgggag cgacgacgac     2220

cuguucauca gcaaguggcu cgucgagcac auccggggcu ucaagaaggc cugcgaggac     2280

ucccugaaga uccagaagga caaccgcggc cugcucaacc acaagaucaa caucgcccgg     2340

aacaccaagg ggaagugcga gaaggagauc uucaaccuga ucugcaagau cgagggcagc     2400

gaggacaaga aggggaacua caagcacggc cuggccuacg agcucggggu gcugcuguuc     2460

ggcgagccga acgaggccuc caagcccgag uucgaccgca agaucaagaa guucaacagc     2520

aucuacuccu ucgcgcagau ccagcagauc gccuucgccg agcggaaggg caacgccaac     2580

acgugcgcgg ucugcagcgc cgacaacgcc caccgcaugc agcagaucaa gaucaccgag     2640

cccguggagg acaacaagga caagaucauc cucuccgcca aggcccagcg gcugcccgcg     2700

aucccgaccc gcaucgugga cggggccguc aagaagaugg ccaccauccu ggccaagaac     2760

aucguggacg acaacuggca gaacaucaag cagguccuca gcgccaagca ccagcugcac     2820

auccccauca ucaccgaguc caacgcguuc gaguucgagc ccgcccuggc cgacgugaag     2880

ggcaagagcc ucaaggaccg gcgcaagaag gcccuggagc ggaucucccc cgagaacauc     2940

uucaaggaca agaacaaccg caucaaggag uucgccaagg ggaucagcgc guacuccggc     3000

gccaaccuga ccgacgggga cuucgacggc gccaaggagg agcucgacca caucaucccg     3060

cggagccaca agaaguacgg cacgcugaac gacgaggcca accugaucug cgugacccgc     3120

ggggacaaca agaacaaggg caaccgcauc uucugccucc gggaccuggc ggacaacuac     3180

aagcugaagc aguucgagac caccgacgac cucgagaucg agaagaagau cgccgacacg     3240

aucugggacg ccaacaagaa ggacuucaag uucgggaacu accgcuccuu caucaaccug     3300

acgccccagg agcagaaggc cuuccggcac gcccuguucc ucgcggacga gaaccccauc     3360

aagcaggccg ugauccgcgc caucaacaac cggaaccgca ccuucgucaa cggcacccag     3420

cgguacuucg ccgaggugcu ggccaacaac aucuaccugc gcgcgaagaa ggagaaccuc     3480

aacaccgaca agaucagcuu cgacuacuuc ggcaucccca ccaucgggaa cggccggggg     3540

aucgccgaga uccgccagcu guacgagaag guggacuccg acauccaggc cuacgccaag     3600

ggcgacaagc cgcaggccag cuacagccac cugaucgacg cgaugcucgc cuucugcauc     3660

gccgccgacg agcaccggaa cgacgggucc aucggccugg agaucgacaa gaacuacagc     3720

cuguaccccc ucgacaagaa caccggcgag gucuucacca aggacaucuu cucccagauc     3780

aagaucaccg acaacgaguu cagcgacaag aagcuggugc gcaagaaggc gaucgagggg     3840

uucaacacgc accggcagau gacccgcgac ggcaucuacg ccgagaacua ccugcccauc     3900

cucauccaca aggagcugaa cgaggugcgg aagggguaca ccuggaagaa cuccgaggag     3960

aucaagaucu ucaagggcaa gaaguacgac auccagcagc ugaacaaccu cgucuacugc     4020

cugaaguucg uggacaagcc gaucagcauc gacauccaga ucuccacccu ggaggagcuc     4080

cgcaacaucc ugacgaccaa caacaucgcc gccaccgccg aguacuacua caucaaccug     4140

aagacccaga agcuccacga guacuacauc gagaacuaca acacggcgcu gggguacaag     4200

aaguacagca aggagaugga guuccugcgg ucccucgccu accgcagcga gcgggugaag     4260

aucaagucca ucgacgacgu caagcagguc cuggacaagg acagcaacuu caucaucggc     4320

aagaucaccc ugcccuucaa gaaggagugg cagcgccucu accgggagug gcagaacacc     4380

accaucaagg acgacuacga guuccugaag uccuucuuca acgucaagag caucacgaag     4440

cugcacaaga aggugcgcaa ggacuucucc cuccccauca gcaccaacga gggcaaguuc     4500

cuggugaagc gcaagaccug ggacaacaac uucaucuacc agauccugaa cgacuccgac     4560

agccgggccg acgggaccaa gcccuucauc ccggccuucg acaucuccaa gaacgagauc     4620

guggaggcca ucaucgacag cuucacgagc aagaacaucu ucuggcuccc caagaacauc     4680

gagcugcaga agguggacaa caagaacauc uucgcgaucg acaccuccaa gugguucgag     4740

guggagaccc ccagcgaccu gcgcgacauc ggcaucgcca ccauccagua caagaucgac     4800

aacaacuccc ggcccaaggu gcgcgucaag cuggacuacg ugaucgacga cgacagcaag     4860

aucaacuacu ucaugaacca cucccuccuc aagagccggu acccggacaa ggugcuggag     4920

auccugaagc aguccacgau caucgaguuc gagagcuccg gguucaacaa gaccaucaag     4980

gagaugcugg gcaugaagcu ggccgggauc uacaacgaga ccagcaacaa caagcgcccc     5040

gccgccacca agaaggcggg ccaggccaag aagaagaagu aaggacuagu cccuguuccc     5100

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     5160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     5220

uu                                                                    5222


<210>  2380
<211>  3494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2380
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaag cggaacuaca uccugggccu cgacaucggg      180

aucaccagcg ugggcuacgg gaucaucgac uacgagaccc gcgacgugau cgacgccggc      240

guccggcugu ucaaggaggc caacguggag aacaacgagg gccgccgguc caagcgcggg      300

gcccggcgcc ugaagcggcg ccggcgccac cggauccagc gcgugaagaa gcuccuguuc      360

gacuacaacc ugcucacgga ccacagcgag cuguccggca ucaaccccua cgaggcccgg      420

gucaaggggc ugagccagaa gcucuccgag gaggaguuca gcgcggcccu gcugcaccuc      480

gccaagcgcc ggggcgugca caacgugaac gaggucgagg aggacaccgg gaacgagcug      540

uccaccaagg agcagaucag ccgcaacucc aaggcccugg aggagaagua cguggcggag      600

cuccagcugg agcggcugaa gaaggacggc gaggugcgcg gcagcaucaa ccgguucaag      660

accuccgacu acgucaagga ggccaagcag cuccugaagg ugcagaaggc cuaccaccag      720

cuggaccaga gcuucaucga cacguacauc gaccuccugg agacccgccg caccuacuac      780

gaggggcccg gcgagggguc ccccuucggc uggaaggaca ucaaggagug guacgagaug      840

cugaugggcc acugcaccua cuucccggag gagcuccgga gcgugaagua cgccuacaac      900

gccgaccugu acaacgcgcu gaacgaccuc aacaaccugg ucaucacgcg cgacgagaac      960

gagaagcugg aguacuacga gaaguuccag aucaucgaga acguguucaa gcagaagaag     1020

aagcccaccc ucaagcagau cgccaaggag auccugguga acgaggagga caucaagggg     1080

uaccggguca ccuccaccgg caagcccgag uucacgaacc ugaaggugua ccacgacauc     1140

aaggacauca ccgcccgcaa ggagaucauc gagaacgccg agcuccugga ccagaucgcc     1200

aagauccuga ccaucuacca gagcuccgag gacauccagg aggagcucac caaccugaac     1260

agcgagcuga cgcaggagga gaucgagcag aucuccaacc ucaaggggua caccggcacc     1320

cacaaccuga gccugaaggc gaucaaccuc auccuggacg agcuguggca caccaacgac     1380

aaccagaucg ccaucuucaa ccggcucaag cuggugccca agaaggucga ccugagccag     1440

cagaaggaga ucccgacgac ccucguggac gacuucaucc ugucccccgu ggucaagcgc     1500

agcuucaucc aguccaucaa ggugaucaac gccaucauca agaaguacgg gcugcccaac     1560

gacaucauca ucgagcuggc ccgggagaag aacagcaagg acgcccagaa gaugaucaac     1620

gagaugcaga agcgcaaccg gcagaccaac gagcgcaucg aggagaucau ccggaccacg     1680

ggcaaggaga acgcgaagua ccucaucgag aagaucaagc ugcacgacau gcaggagggc     1740

aagugccugu acucccucga ggccaucccc cuggaggacc ugcucaacaa cccguucaac     1800

uacgaggucg accacaucau cccccgcagc guguccuucg acaacagcuu caacaacaag     1860

gugcugguca agcaggagga gaacuccaag aaggggaacc ggacccccuu ccaguaccug     1920

agcuccagcg acuccaagau cagcuacgag accuucaaga agcacauccu caaccuggcc     1980

aagggcaagg ggcgcaucuc caagaccaag aaggaguacc ugcucgagga gcgggacauc     2040

aaccgcuuca gcgugcagaa ggacuucauc aaccggaacc ugguggacac gcgcuacgcc     2100

acccggggcc ugaugaaccu ccugcgcucc uacuuccgcg ucaacaaccu ggacgugaag     2160

gucaagagca ucaacggggg cuucaccucc uuccuccggc gcaaguggaa guucaagaag     2220

gagcggaaca agggcuacaa gcaccacgcc gaggacgcgc ugaucaucgc caacgccgac     2280

uucaucuuca aggaguggaa gaagcuggac aaggccaaga aggugaugga gaaccagaug     2340

uucgaggaga agcaggcgga gagcaugccc gagaucgaga ccgagcagga guacaaggag     2400

aucuucauca cgccgcacca gaucaagcac aucaaggacu ucaaggacua caaguacagc     2460

caccgcgugg acaagaagcc caaccgggag cucaucaacg acacccugua cuccacccgc     2520

aaggacgaca aggggaacac ccugaucgug aacaaccuca acggccugua cgacaaggac     2580

aacgacaagc ugaagaagcu caucaacaag agccccgaga agcugcugau guaccaccac     2640

gacccccaga cguaccagaa gcucaagcug aucauggagc aguacgggga cgagaagaac     2700

ccgcuguaca aguacuacga ggagaccggc aacuaccuca ccaaguacuc caagaaggac     2760

aacgggcccg ucaucaagaa gaucaaguac uacggcaaca agcugaacgc ccaccuggac     2820

aucaccgacg acuaccccaa cagccggaac aaggugguga agcucucccu gaagcccuac     2880

cgcuucgacg ucuaccugga caacggcgug uacaaguucg ugaccgucaa gaaccucgac     2940

gugaucaaga aggagaacua cuacgaggug aacagcaagu gcuacgagga ggccaagaag     3000

cugaagaaga ucuccaacca ggccgaguuc aucgccagcu ucuacaacaa cgaccugauc     3060

aagaucaacg gggagcucua ccgggucauc ggcgugaaca acgaccugcu gaaccgcauc     3120

gaggugaaca ugaucgacau cacguaccgg gaguaccucg agaacaugaa cgacaagcgc     3180

ccgccccgga ucaucaagac caucgcgucc aagacccaga gcaucaagaa guacuccacc     3240

gacauccugg ggaaccugua cgaggucaag agcaagaagc acccccagau caucaagaag     3300

ggcaagcgcc ccgccgccac gaagaaggcc ggccaggcca agaagaagaa guaaggacua     3360

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     3420

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaag aauu                                                       3494


<210>  2381
<211>  4562
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2381
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgcug uucaacaagu gcaucaucau cagcaucaac      180

cucgacuucu ccaacaagga gaagugcaug accaagcccu acagcaucgg ccuggacauc      240

gggaccaacu ccgugggcug ggccgugauc acggacaacu acaagguccc cagcaagaag      300

augaaggugc uggggaacac cuccaagaag uacaucaaga agaaccuccu gggcgugcug      360

cuguucgaca gcggcaucac cgccgagggg cggcgccuga agcggaccgc ccgccggcgc      420

uacacgcggc gccggaaccg cauccuguac cuccaggaga ucuucuccac cgagauggcc      480

acccuggacg acgcguucuu ccagcggcug gacgacagcu uccucguccc cgacgacaag      540

cgcgacucca aguacccgau cuucggcaac cugguggagg agaaggugua ccacgacgag      600

uuccccacca ucuaccaccu ccggaaguac cucgccgaca gcacgaagaa ggccgaccug      660

cgccuggucu accucgcccu ggcgcacaug aucaaguacc gggggcacuu ccugaucgag      720

ggcgaguuca acuccaagaa caacgacauc cagaagaacu uccaggacuu ccucgacacc      780

uacaacgcca ucuucgagag cgaccugucc cuggagaaca gcaagcagcu cgaggagauc      840

gugaaggaca agaucuccaa gcuggagaag aaggaccgca uccugaagcu guuccccggg      900

gagaagaaca gcggcaucuu cuccgaguuc cugaagcuga ucgugggcaa ccaggccgac      960

uuccggaagu gcuucaaccu cgacgagaag gccagccugc acuucuccaa ggagagcuac     1020

gacgaggacc uggagacccu ccugggguac aucggcgacg acuacagcga cgucuuccug     1080

aaggccaaga agcucuacga cgcgauccug cuguccgggu uccucaccgu gacggacaac     1140

gagaccgagg cgccccugag cuccgccaug aucaagcgcu acaacgagca caaggaggac     1200

cuggcccucc ugaaggagua cauccggaac aucagccuga agaccuacaa cgagguguuc     1260

aaggacgaca ccaagaacgg cuacgccggc uacaucgacg ggaagacgaa ccaggaggac     1320

uucuacgucu accucaagaa ccugcuggcc gaguucgagg gcgccgacua cuuccucgag     1380

aagaucgacc gcgaggacuu ccugcggaag cagcgcaccu ucgacaacgg guccaucccg     1440

uaccagaucc accuccagga gaugcgcgcc auccuggaca agcaggccaa guucuacccc     1500

uuccucgcca agaacaagga gcggaucgag aagauccuga ccuuccgcau ccccuacuac     1560

gugggccccc uggcgcgggg gaacagcgac uucgccuggu ccauccgcaa gcggaacgag     1620

aagaucaccc cguggaacuu cgaggacgug aucgacaagg agagcuccgc cgaggccuuc     1680

aucaaccgca ugacgagcuu cgaccucuac cugcccgagg agaagguccu gcccaagcac     1740

ucccuccugu acgagaccuu caacguguac aacgagcuga ccaaggugcg guucaucgcc     1800

gagagcaugc gcgacuacca guuccucgac uccaagcaga agaaggacau cguccggcug     1860

uacuucaagg acaagcgcaa ggugaccgac aaggacauca ucgaguaccu gcacgcgauc     1920

uacggcuacg acggcaucga gcucaagggg aucgagaagc aguucaacag cucccugagc     1980

acguaccacg accugcucaa caucaucaac gacaaggagu uccuggacga cuccagcaac     2040

gaggccauca ucgaggagau cauccacacc cugaccaucu ucgaggaccg ggagaugauc     2100

aagcagcgcc ucuccaaguu cgagaacauc uucgacaaga gcgugcugaa gaagcugagc     2160

cggcgccacu acaccggcug ggggaagcuc uccgccaagc ugaucaacgg cauccgggac     2220

gagaaguccg ggaacacgau ccuggacuac cucaucgacg acggcaucag caaccgcaac     2280

uucaugcagc ugauccacga cgacgcccug agcuucaaga agaagaucca gaaggcgcag     2340

aucaucggcg acgaggacaa ggggaacauc aaggaggucg ugaagucccu ccccggcagc     2400

ccggccauca agaaggggau ccugcagucc aucaagaucg ucgacgagcu ggugaaggug     2460

augggcgggc ggaagcccga gagcaucguc guggagaugg cccgcgagaa ccaguacacc     2520

aaccagggca aguccaacag ccagcagcgg cucaagcgcc uggagaaguc ccugaaggag     2580

cucggcagca agauccugaa ggagaacauc cccgccaagc uguccaagau cgacaacaac     2640

gcccuccaga acgaccgccu guaccuguac uaccuccaga acgggaagga cauguacacc     2700

ggcgacgacc uggacaucga ccggcugagc aacuacgaca ucgaccacau caucccccag     2760

gcguuccuca aggacaacuc caucgacaac aaggugcugg ucagcuccgc cagcaaccgc     2820

gggaaguccg acgacuuccc gagccuggag guggugaaga agcggaagac cuucugguac     2880

cagcuccuca agagcaagcu gaucucccag cgcaaguucg acaaccugac gaaggccgag     2940

cggggcggcc ugcugcccga ggacaaggcc ggguucaucc agcgccagcu cgucgagacc     3000

cggcagauca ccaagcacgu ggcccgccug cuggacgaga aguucaacaa caagaaggac     3060

gagaacaacc gggcggugcg caccgucaag aucaucacgc ucaagagcac ccuggugucc     3120

caguuccgga aggacuucga gcuguacaag gugcgcgaga ucaacgacuu ccaccacgcc     3180

cacgacgccu accucaacgc cgucaucgcc agcgcgcugc ugaagaagua ccccaagcuc     3240

gagcccgagu ucguguacgg cgacuacccg aaguacaacu ccuuccggga gcgcaagagc     3300

gccaccgaga agguguacuu cuacuccaac aucaugaaca ucuucaagaa guccaucagc     3360

cuggccgacg ggcgggucau cgagcgcccc cugaucgagg ugaacgagga gaccggcgag     3420

agcgugugga acaaggaguc cgaccucgcc accguccggc gcgugcugag cuacccccag     3480

gugaacgucg ugaagaaggu ggaggagcag aaccacgggc uggaccgggg caagcccaag     3540

ggccucuuca acgcgaaccu guccagcaag ccgaagccca acuccaacga gaaccugguc     3600

ggggccaagg aguaccucga ccccaagaag uacggcgggu acgccggcau cagcaacucc     3660

uucgccgugc uggugaaggg gacgaucgag aagggcgcca agaagaagau caccaacguc     3720

cuggaguucc agggcaucag cauccucgac cgcaucaacu accggaagga caagcugaac     3780

uuccugcucg agaaggggua caaggacauc gagcugauca ucgagcugcc gaaguacagc     3840

cucuucgagc uguccgacgg cagccgccgc augcuggcgu ccauccucag caccaacaac     3900

aagcgcgggg agauccacaa gggcaaccag aucuuccugu cccagaaguu cgugaagcug     3960

cucuaccacg ccaagcggau cagcaacacc aucaacgaga accaccggaa guacguggag     4020

aaccacaaga aggaguucga ggagcuguuc uacuacaucc uggaguucaa cgagaacuac     4080

gucggggcca agaagaacgg caagcuccug aacuccgccu uccagagcug gcagaaccac     4140

uccaucgacg agcugugcag cuccuucauc ggccccacgg ggagcgagcg caagggccuc     4200

uucgagcuga ccucccgggg gagcgccgcg gacuucgagu uccugggcgu gaagaucccc     4260

cgcuaccggg acuacacccc cuccagccuc cugaaggacg ccacccugau ccaccagucc     4320

gugacggggc uguacgagac ccgcaucgac cucgccaagc ugggcgaggg caagcggccg     4380

gccgccacca agaaggcggg gcaggccaag aagaagaagu aaggacuagu cccuguuccc     4440

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     4560

uu                                                                    4562


<210>  2382
<211>  3638
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2382
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgugg uacgccagcc ugauguccgc ccaccaccuc      180

cgggugggca ucgacguggg gacccacagc gucggccugg ccacccugcg cguggacgac      240

cacgggacgc ccaucgagcu ccuguccgcc cugagccaca uccacgacuc cggcgugggc      300

aaggagggga agaaggacca cgacacccgg aagaagcuca gcggcaucgc gcgccgggcc      360

cgccggcugc ugcaccaccg ccggacccag cuccagcagc uggacgaggu ccugcgcgac      420

cucggguucc ccauccccac cccgggcgag uuccuggacc ugaacgagca gacggacccc      480

uaccgggugu ggcgcgugcg ggcccgccuc gucgaggaga agcugcccga ggagcugcgg      540

gggcccgcca ucuccauggc ggugcgccac aucgcccggc accgcggcug gcggaacccg      600

uacagcaagg uggagucccu ccugagcccc gccgaggagu cccccuucau gaaggcccug      660

cgcgagcgga uccucgccac caccggcgag guccuggacg acgggaucac ccccggccag      720

gcgauggccc agguggcccu gacgcacaac aucagcaugc gcgggccgga gggcauccuc      780

ggcaagcugc accaguccga caacgccaac gagauccgca agaucugcgc ccggcagggg      840

gugagccccg acgucugcaa gcagcugcuc cgcgcggugu ucaaggccga cagcccccgg      900

ggcuccgccg ugucccgcgu cgcccccgac ccgcugcccg ggcagggcag cuuccggcgc      960

gcccccaagu gcgaccccga guuccagcgg uuccgcauca ucuccaucgu ggcgaaccug     1020

cggaucagcg agaccaaggg ggagaaccgc ccgcucaccg ccgacgagcg gcgccacgug     1080

gucaccuucc ugacggagga cagccaggcc gaccugaccu ggguggacgu ggccgagaag     1140

cucggcgucc accggcgcga ccugcggggc accgccgugc acaccgacga cggggagcgc     1200

uccgcggccc ggccgcccau cgacgccacg gaccgcauca ugcggcagac caagaucagc     1260

ucccugaaga ccugguggga ggaggccgac agcgagcagc gcggcgcgau gauccgcuac     1320

cucuacgagg accccaccga cuccgagugc gccgagauca ucgccgagcu gcccgaggag     1380

gaccaggcca agcuggacag ccuccaccuc cccgccgggc gggcggccua cucccgcgag     1440

agccugacgg cccuguccga ccacaugcug gccaccaccg acgaccugca cgaggcccgg     1500

aagcgccucu ucggcgucga cgacagcugg gcgccgcccg ccgaggccau caacgccccc     1560

guggggaacc ccuccgugga ccggacccug aagaucgucg gccgcuaccu gagcgccgug     1620

gaguccaugu ggggcacgcc cgaggugauc cacgucgagc acgugcggga cggguucacc     1680

agcgagcgca uggcggacga gcgggacaag gccaaccgcc ggcgcuacaa cgacaaccag     1740

gaggccauga agaagaucca gcgggacuac ggcaaggagg gguacaucuc ccgcggcgac     1800

aucgugcggc ucgacgcccu ggagcugcag gggugcgcgu gccuguacug cggcaccacc     1860

aucggcuacc acacgugcca gcucgaccac aucgucccgc aggccgggcc cggcagcaac     1920

aaccgccggg ggaaccuggu ggccgugugc gagcgcugca accgguccaa gagcaacacc     1980

cccuucgccg ucugggccca gaagugcggc aucccccacg ugggcgugaa ggaggcgauc     2040

gggcgcgucc ggggcuggcg caagcagacc ccgaacaccu ccagcgagga ccugacgcgc     2100

cucaagaagg aggugaucgc ccggcugcgc cggacccagg aggaccccga gaucgacgag     2160

cgcagcaugg aguccguggc cuggauggcc aacgagcugc accaccggau cgccgcggcc     2220

uaccccgaga ccaccgucau gguguaccgc gggagcauca cggccgccgc ccggaaggcg     2280

gccggcaucg acucccgcau caaccucauc ggggagaagg gccggaagga ccgcaucgac     2340

cggcgccacc acgccgugga cgccagcguc guggcgcuga uggaggccuc cguggccaag     2400

acccuggccg agcggagcuc ccuccgcggc gagcagcggc ugaccgggaa ggagcagacc     2460

uggaagcagu acaccggcag cacggucggg gcccgcgagc acuucgagau guggcggggc     2520

cacaugcugc accucaccga gcuguucaac gagcgccugg cggaggacaa gguguacgug     2580

acccagaaca uccggcuccg ccuguccgac gggaacgccc acaccgucaa ccccagcaag     2640

cugguguccc accgccucgg cgacggccug acggugcagc agaucgaccg ggccugcacc     2700

ccggcccugu ggugcgcccu cacccgcgag aaggacuucg acgagaagaa cgggcugccc     2760

gcgcgggagg accgcgccau ccggguccac ggccacgaga ucaagagcuc cgacuacauc     2820

cagguguuca gcaagcgcaa gaagaccgac uccgaccggg acgagacgcc cuucggggcc     2880

aucgccgugc gcggcggguu cgucgagauc ggccccagca uccaccacgc ccggaucuac     2940

cgcguggagg gcaagaagcc gguguacgcg augcugcggg ucuucaccca cgaccuccug     3000

ucccagcgcc acggggaccu guucagcgcc gugaucccgc cccagagcau cuccaugcgg     3060

ugcgccgagc ccaagcuccg caaggccauc accaccggca acgccacgua ccuggggugg     3120

guggucgugg gcgacgagcu ggagaucaac guggacagcu ucaccaagua cgcgaucggg     3180

cgguuccucg aggacuuccc caacaccacc cgcuggcgga ucugcggcua cgacacgaac     3240

uccaagcuga cccugaagcc caucguccuc gccgccgagg gccuggagaa ccccagcucc     3300

gccgugaacg agaucgugga gcugaagggg uggcgcgucg cgaucaacgu gcucaccaag     3360

gugcacccca ccgucgugcg gcgcgacgcc cugggccggc cgcgcuacag cucccgcagc     3420

aaccugccca cguccuggac caucgagaag cggcccgccg ccaccaagaa ggccgggcag     3480

gcgaagaaga agaaguaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu     3540

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                             3638


<210>  2383
<211>  4499
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2383
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgacc aagcccuaca gcaucggccu ggacaucggg      180

accaacuccg ugggcugggc cgugacgacc gacaacuaca agguccccag caagaagaug      240

aaggugcucg ggaacaccuc caagaaguac aucaagaaga accugcuggg cgugcuccug      300

uucgacagcg gcaucaccgc cgaggggcgg cgccugaagc ggacggcccg ccggcgcuac      360

acccggcgcc ggaaccgcau ccucuaccug caggagaucu ucuccaccga gauggccacc      420

cuggacgacg cguucuucca gcggcucgac gacagcuucc ugguccccga cgacaagcgc      480

gacuccaagu acccgaucuu cggcaaccug guggaggaga aggccuacca cgacgaguuc      540

cccacgaucu accaccuccg gaaguaccug gccgacagca ccaagaaggc cgaccugcgc      600

cucguguacc uggcgcuggc ccacaugauc aaguaccggg ggcacuuccu caucgagggc      660

gaguucaacu ccaagaacaa cgacauccag aagaacuucc aggacuuccu ggacaccuac      720

aacgccaucu ucgagagcga ccugucccuc gagaacagca agcagcugga ggagaucguc      780

aaggacaaga ucuccaagcu ggagaagaag gaccgcaucc ucaagcuguu ccccggggag      840

aagaacagcg gcaucuucuc cgaguuccug aagcucaucg ugggcaacca ggccgacuuc      900

cggaagugcu ucaaccugga cgagaaggcc agccugcacu ucuccaagga gagcuacgac      960

gaggaccucg agacccugcu gggguacauc ggcgacgacu acuccgacgu guuccucaag     1020

gcgaagaagc uguacgacgc cauccugcuc agcggguucc ugacggucac cgacaacgag     1080

accgaggcgc cccugagcuc cgccaugauc aagcgcuaca acgagcacaa ggaggaccuc     1140

gcccugcuga aggaguacau ccggaacauc agccucaaga ccuacaacga gguguucaag     1200

gacgacacga agaacggcua cgccggcuac aucgacggga agaccaacca ggaggacuuc     1260

uacguguacc ugaagaagcu gcucgccgag uucgagggcg ccgacuacuu ccuggagaag     1320

aucgaccgcg aggacuuccu gcggaagcag cgcaccuucg acaacggguc caucccguac     1380

cagauccacc uccaggagau gcgcgccauc cuggacaagc aggccaaguu cuaccccuuc     1440

cuggcgaaga acaaggagcg gaucgagaag auccugaccu uccgcauccc cuacuacguc     1500

ggcccccucg cccgggggaa cagcgacuuc gccuggucca uccgcaagcg gaacgagaag     1560

aucacgccgu ggaacuucga ggacgugauc gacaaggaga gcuccgccga ggccuucauc     1620

aaccgcauga ccagcuucga ccuguaccug cccgaggaga aggugcuccc caagcacucc     1680

cugcuguacg agaccuucaa cgucuacaac gagcucacca aggugcgguu caucgcggag     1740

agcaugcgcg acuaccaguu ccuggacucc aagcagaaga aggacaucgu gcggcuguac     1800

uucaaggaca agcgcaaggu cacggacaag gacaucaucg aguaccucca cgccaucuac     1860

ggcuacgacg gcaucgagcu gaaggggauc gagaagcagu ucaacagcuc ccugagcacc     1920

uaccacgacc uccugaacau caucaacgac aaggaguucc uggacgacuc cagcaacgag     1980

gccaucaucg aggagaucau ccacacccuc accaucuucg aggaccggga gaugaucaag     2040

cagcgccugu ccaaguucga gaacaucuuc gacaagagcg ugcugaagaa gcucucccgg     2100

cgccacuaca cgggcugggg gaagcugagc gccaagcuga ucaacggcau ccgggacgag     2160

aaguccggga acaccauccu cgacuaccug aucgacgacg gcaucagcaa ccgcaacuuc     2220

augcagcuga uccacgacga cgcgcucagc uucaagaaga agauccagaa ggcccagauc     2280

aucggcgacg aggacaaggg gaacaucaag gaggugguca agucccugcc cggcagcccg     2340

gccaucaaga aggggauccu gcaguccauc aagaucgugg acgagcucgu caaggugaug     2400

ggcgggcgga agcccgagag caucgugguc gagauggccc gcgagaacca guacaccaac     2460

cagggcaagu ccaacagcca gcagcggcug aagcgccugg agaagucccu caaggagcug     2520

ggcagcaaga uccugaagga gaacaucccc gccaagcucu ccaagaucga caacaacgcg     2580

cugcagaacg accgccugua ccucuacuac cugcagaacg ggaaggacau guacaccggc     2640

gacgaccugg acaucgaccg gcucagcaac uacgacaucg accacaucau cccccaggcc     2700

uuccugaagg acaacuccau cgacaacaag gugcugguga gcuccgccag caaccgcggg     2760

aaguccgacg acgucccgag ccucgaggug gugaagaagc ggaagacguu cugguaccag     2820

cugcucaaga gcaagcugau cucccagcgc aaguucgaca accugaccaa ggccgagcgg     2880

ggcggccuga gccccgagga caaggccggg uucauccagc gccagcucgu cgagacccgg     2940

cagaucacca agcacguggc gcgccugcug gacgagaagu ucaacaacaa gaaggacgag     3000

aacaaccggg ccgugcgcac ggucaagauc aucacccuca aguccacccu ggugagccag     3060

uuccggaagg acuucgagcu guacaaggug cgcgagauca acgacuucca ccacgcccac     3120

gacgccuacc ucaacgccgu cguggcgucc gcccugcuga agaaguaccc caagcucgag     3180

cccgaguucg uguacggcga cuacccgaag uacaacagcu uccgggagcg caaguccgcc     3240

accgagaagg ucuacuucua cagcaacauc augaacaucu ucaagaaguc caucagccug     3300

gccgacgggc gggugaucga gcgcccccug aucgagguga acgaggagac cggcgagucc     3360

gucuggaaca aggagagcga ccucgcgacg gugcggcgcg ugcuguccua cccccagguc     3420

aacgugguga agaaggucga ggagcagaac cacgggcugg accggggcaa gcccaagggc     3480

cucuucaacg ccaaccugag cuccaagccg aagcccaaca gcaacgagaa ccuggugggg     3540

gccaaggagu accucgaccc caagaaguac ggcggguacg ccggcaucuc caacagcuuc     3600

accgugcugg ucaaggggac caucgagaag ggcgccaaga agaagaucac caacgugcug     3660

gaguuccagg gcaucuccau ccucgaccgc aucaacuacc ggaaggacaa gcugaacuuc     3720

cugcucgaga agggguacaa ggacaucgag cugaucaucg agcugccgaa guacagccuc     3780

uucgagcuga gcgacggcuc ccgccgcaug cuggcgagca uccucuccac gaacaacaag     3840

cgcggggaga uccacaaggg caaccagauc uuccugagcc agaaguucgu gaagcugcuc     3900

uaccacgcca agcggaucuc caacaccauc aacgagaacc accggaagua cgucgagaac     3960

cacaagaagg aguucgagga gcuguucuac uacauccugg aguucaacga gaacuacgug     4020

ggggccaaga agaacggcaa gcuccugaac agcgccuucc aguccuggca gaaccacagc     4080

aucgacgagc ugugcuccag cuucaucggc cccaccgggu ccgagcgcaa gggccucuuc     4140

gagcugacca gccggggguc cgccgcggac uucgaguucc ugggcgugaa gaucccccgc     4200

uaccgggacu acacgcccag cucccuccug aaggacgcca cccugaucca ccagagcguc     4260

accgggcucu acgagacccg caucgaccug gccaagcugg gcgagggcaa gcggccggcc     4320

gccacgaaga aggcggggca ggccaagaag aagaaguaag gacuaguccc uguucccaga     4380

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      4499


<210>  2384
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2384
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcggcc gccuucaagc ccaacagcau caacuacauc      180

cugggccucg acaucgggau cgccuccgug ggcugggcca ugguggagau cgacgaggag      240

gagaacccca uccggcugau cgaccugggg guccgcgugu ucgagcgggc ggaggugccc      300

aagaccggcg acagccucgc cauggcccgc cggcuggccc gcuccguccg gcgccugacc      360

cggcgccggg cgcaccgccu ccugcggacg cgccggcugc ucaagcgcga gggcgugcug      420

caggccgcca acuucgacga gaacgggcug aucaagagcc ucccgaacac ccccuggcag      480

cugcgggccg ccgcgcugga ccgcaagcuc acgccccugg agugguccgc cgugcugcuc      540

caccugauca agcaccgggg cuaccugagc cagcgcaaga acgaggggga gaccgccgac      600

aaggagcucg gcgcccugcu gaaggggguc gccggcaacg cgcacgcccu ccagaccggc      660

gacuuccgga cccccgccga gcuggcccug aacaaguucg agaaggaguc cgggcacauc      720

cgcaaccagc gcagcgacua cucccacacc uucagccgga aggaccucca ggccgagcug      780

auccuccugu ucgagaagca gaaggaguuc ggcaacccgc acguguccgg gggccugaag      840

gagggcaucg agacccugcu caugacgcag cgccccgcgc ugagcgggga cgccgugcag      900

aagaugcugg gccacugcac cuucgagccc gccgagccca aggccgccaa gaacaccuac      960

accgcggagc gguucaucug gcucacgaag cugaacaacc ugcgcauccu cgagcagggg     1020

uccgagcggc cgcugaccga caccgagcgc gccacccuga uggacgagcc cuaccgcaag     1080

agcaagcuca cguacgccca ggcccggaag cugcugggcc ucgaggacac cgcguucuuc     1140

aaggggcugc gguacggcaa ggacaacgcc gaggccucca cccugaugga gaugaaggcc     1200

uaccacgcca ucagccgcgc gcucgagaag gagggccuga aggacaagaa guccccgcug     1260

aaccugagcc ccgagcucca ggacgagauc gggaccgccu ucucccuguu caagacggac     1320

gaggacauca ccggccggcu gaaggaccgc auccagccgg agauccucga ggcccugcug     1380

aagcacauca gcuucgacaa guucguccag aucagccuca aggcccugcg gcgcaucgug     1440

ccccugaugg agcaggggaa gcgguacgac gaggccugcg cggagaucua cggcgaccac     1500

uacgggaaga agaacaccga ggagaagauc uaccucccgc ccauccccgc cgacgagauc     1560

cgcaaccccg ugguccugcg ggcccugucc caggcccgca aggugaucaa cggcgugguc     1620

cggcgcuacg gcagccccgc ccgcauccac aucgagaccg cgcgggaggu ggggaagucc     1680

uucaaggacc gcaaggagau cgagaagcgg caggaggaga accgcaagga ccgggagaag     1740

gccgccgcca aguuccgcga guacuucccc aacuucgugg gcgagcccaa gagcaaggac     1800

auccucaagc ugcggcugua cgagcagcag cacgggaagu gccucuacuc cggcaaggag     1860

aucaaccugg ggcgccugaa cgagaagggc uacgucgaga ucgaccacgc gcuccccuuc     1920

agccggacgu gggacgacuc cuucaacaac aaggugcugg ugcugggcag cgagaaccag     1980

aacaagggga accagacccc cuacgaguac uucaacggca aggacaacuc ccgcgagugg     2040

caggaguuca aggcccgggu cgagaccagc cgcuuccccc gguccaagaa gcagcgcauc     2100

cuccugcaga aguucgacga ggacggguuc aaggagcgga accugaacga cacccgcuac     2160

gugaaccggu uccucugcca guucgucgcc gaccgcaugc ggcugacggg caagggcaag     2220

aagcgcgugu ucgccagcaa cgggcagauc accaaccugc uccggggcuu cugggggcug     2280

cgcaaggugc gcgccgagaa cgaccggcac cacgcgcugg acgccgucgu gguggccugc     2340

uccaccgucg ccaugcagca gaagaucacc cgcuucgugc gguacaagga gaugaacgcc     2400

uucgacggca agacgaucga caaggagacc ggggaggugc uccaccagaa gacccacuuc     2460

ccgcagcccu gggaguucuu cgcgcaggag gucaugaucc gcguguucgg caagcccgac     2520

ggcaagccgg aguucgagga ggccgacacc cuggagaagc ugcggacgcu ccuggccgag     2580

aagcugagcu cccgccccga ggccgugcac gaguacguca cgccccucuu cgugagccgg     2640

gcccccaacc gcaagauguc cgggcagggc cacauggaga ccgucaagag cgcgaagcgg     2700

cuggacgagg ggguguccgu gcugcgcgug ccgcucaccc agcugaagcu gaaggaccuc     2760

gagaagaugg ucaaccggga gcgcgagccc aagcuguacg aggcccugaa ggcccggcuc     2820

gaggcccaca aggacgaccc cgcgaaggcc uucgccgagc ccuucuacaa guacgacaag     2880

gccggcaacc gcacccagca ggucaaggcc gugcgggugg agcaggucca gaagaccggg     2940

gugugggugc gcaaccacaa cggcaucgcg gacaacgcca ccauggugcg gguggacguc     3000

uucgagaagg gcgacaagua cuaccuggug ccgaucuaca gcuggcaggu cgccaagggg     3060

auccugcccg accgcgccgu ggugcagggc aaggacgagg aggacuggca gcucaucgac     3120

gacagcuuca acuucaaguu cucccugcac cccaacgacc ugguggaggu caucaccaag     3180

aaggcccgga uguucgggua cuucgcgagc ugccaccgcg gcaccgggaa caucaacauc     3240

cgcauccacg accucgacca caagaucggc aagaacggca uccuggaggg gaucggcgug     3300

aagacggccc uguccuucca gaaguaccag aucgacgagc ucgggaagga gauccggccc     3360

ugccgccuga agaagcggcc gcccgugcgc aagcggcccg ccgccaccaa gaaggccggc     3420

caggcgaaga agaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3480

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  2385
<211>  4337
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2385
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaag aagcccuaca ccaucggccu ggacaucggg      180

accaacagcg ugggcugggc cgugcucacg gaccaguacg accuggucaa gcggaagaug      240

aagaucgccg gggacuccga gaagaagcag aucaagaaga acuucugggg cgugcgccug      300

uucgacgagg gccagaccgc cgccgaccgg cgcauggcgc ggaccgcccg ccggcgcauc      360

gagcggcgcc ggaaccgcau cagcuaccuc caggggaucu ucgccgagga gauguccaag      420

accgacgcca acuucuucug ccggcugagc gacuccuucu acguggacaa cgagaagcgc      480

aacagccggc accccuucuu cgcgacgauc gaggaggagg ucgaguacca caagaacuac      540

cccaccaucu accaccuccg cgaggagcug gugaacucca gcgagaaggc cgaccugcgg      600

cugguguacc ucgcccuggc ccacaucauc aaguaccgcg gcaacuuccu gaucgagggg      660

gcccucgaca cccagaacac cuccgucgac ggcaucuaca agcaguucau ccagacguac      720

aaccaggugu ucgcgagcgg gaucgaggac ggcucccuga agaagcugga ggacaacaag      780

gacguggcca agauccucgu cgagaaggug acccggaagg agaagcugga gcgcauccug      840

aagcucuacc cgggcgagaa guccgccggg auguucgccc aguucaucag ccugaucgug      900

ggcagcaagg ggaacuucca gaagcccuuc gaccugaucg agaaguccga caucgagugc      960

gccaaggaca gcuacgagga ggaccucgag ucccugcugg cgcucaucgg cgacgaguac     1020

gccgagcugu ucgucgccgc caagaacgcc uacagcgcgg uggugcuguc cagcaucauc     1080

accgucgccg agaccgagac gaacgccaag cucagcgccu ccaugaucga gcgguucgac     1140

acccacgagg aggaccuggg cgagcugaag gccuucauca agcuccaccu ccccaagcac     1200

uacgaggaga ucuucagcaa caccgagaag cacggguacg cgggcuacau cgacgggaag     1260

accaagcagg ccgacuucua caaguacaug aagaugacgc uggagaacau cgagggcgcc     1320

gacuacuuca ucgccaagau cgagaaggag aacuuccugc gcaagcagcg caccuucgac     1380

aacggggcga ucccccacca gcugcaccug gaggagcucg aggccauccu gcaccagcag     1440

gccaaguacu acccguuccu gaaggagaac uacgacaaga ucaagucccu cgugaccuuc     1500

cggauccccu acuucguggg cccccuggcc aacggccaga gcgaguucgc cuggcugacc     1560

cgcaaggcgg acggggagau ccggcccugg aacaucgagg agaaggucga cuucggcaag     1620

uccgccgugg acuucaucga gaagaugacg aacaaggaca ccuaccuccc gaaggagaac     1680

gugcugccca agcacagccu gugcuaccag aaguaccucg ucuacaacga gcugaccaag     1740

gugcgcuaca ucaacgacca ggggaagacc uccuacuuca gcggccagga gaaggagcag     1800

aucuucaacg accuguucaa gcagaagcgg aaggucaaga agaaggaccu cgagcuguuc     1860

cugcgcaaca ugucccacgu ggagagcccc acgaucgagg ggcuggagga cuccuucaac     1920

agcuccuaca gcaccuacca cgaccuccug aaggugggca ucaagcagga gauccuggac     1980

aaccccguca acaccgagau gcucgagaac aucgugaaga uccugaccgu guucgaggac     2040

aagcggauga ucaaggagca gcugcagcag uucuccgacg uccucgacgg cguggugcug     2100

aagaagcugg agcgccggca cuacacgggg uggggccgcc ucagcgccaa gcugcugaug     2160

gggauccggg acaagcaguc ccaccucacc auccuggacu accugaugaa cgacgacggc     2220

cucaaccgca accugaugca gcugaucaac gacagcaacc ucuccuucaa gagcaucauc     2280

gagaaggagc aggucaccac cgccgacaag gacauccaga gcaucguggc cgaccuggcg     2340

ggguccccgg ccaucaagaa gggcauccug cagagccuca agaucgugga cgagcugguc     2400

uccgugaugg gcuacccgcc ccagacgauc guggucgaga uggcccggga gaaccagacc     2460

accgggaagg gcaagaacaa cagccgcccc cgguacaagu cccuggagaa ggccaucaag     2520

gaguucggga gccagauccu caaggagcac cccaccgaca accaggagcu gcgcaacaac     2580

cggcuguacc ucuacuaccu gcagaacggc aaggacaugu acacgggcca ggaccuggac     2640

auccacaacc ucuccaacua cgacaucgac cacaucgugc cccagagcuu caucaccgac     2700

aacuccaucg acaaccuggu gcugaccagc uccgccggga accgcgagaa gggcgacgac     2760

gucccgcccc ucgagaucgu gcggaagcgc aagguguucu gggagaagcu guaccagggg     2820

aaccugauga gcaagcgcaa guucgacuac cucaccaagg cggagcgggg cgggcugacc     2880

gaggccgaca aggcccgcuu cauccaccgg cagcuggucg agacgcgcca gaucaccaag     2940

aacguggcca acauccucca ccagcgguuc aacuacgaga aggacgacca cggcaacacc     3000

augaagcagg uccgcaucgu gacccugaag uccgcgcugg ugagccaguu ccggaagcag     3060

uuccagcucu acaaggugcg cgacgucaac gacuaccacc acgcccacga cgccuaccug     3120

aacggcgugg uggccaacac gcugcucaag gucuaccccc agcuggagcc cgaguucgug     3180

uacggggacu accaccaguu cgacugguuc aaggccaaca aggcgaccgc caagaagcag     3240

uucuacacca acaucaugcu guucuucgcc cagaaggacc ggaucaucga cgagaacggc     3300

gagauccucu gggacaagaa guaccuggac accgugaaga aggucauguc cuaccgccag     3360

augaacaucg ugaagaagac ggagauccag aagggggagu ucagcaaggc caccaucaag     3420

cccaagggca acuccagcaa gcugaucccc cggaagacca acugggaccc gaugaaguac     3480

gggggccucg acagccccaa cauggccuac gcggugguca ucgaguacgc caagggcaag     3540

aacaagcugg uguucgagaa gaagaucauc cgcgugacca ucauggagcg gaaggccuuc     3600

gagaaggacg agaaggccuu ccuggaggag cagggguacc gccagcccaa gguccucgcc     3660

aagcugccga aguacacgcu guacgagugc gaggagggcc ggcgccggau gcucgcgucc     3720

gccaacgagg cccagaaggg gaaccagcag guccugccca accaccuggu gacccuccug     3780

caccacgccg cgaacugcga ggugagcgac ggcaaguccc uggacuacau cgagagcaac     3840

cgcgagaugu ucgccgagcu ccuggcccac guguccgagu ucgccaagcg guacacccug     3900

gccgaggcga accucaacaa gaucaaccag cuguucgagc agaacaagga gggggacauc     3960

aaggccaucg cccagagcuu cguggaccug auggccuuca acgccauggg cgcgcccgcc     4020

uccuucaagu ucuucgagac cacgaucgag cgcaagcggu acaacaaccu caaggagcug     4080

cugaacagca ccaucaucua ccaguccauc accggccucu acgagagccg caagcgccug     4140

gacgacaagc ggcccgccgc caccaagaag gccgggcagg cgaagaagaa gaaguaagga     4200

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4260

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aagaauu                                                    4337


<210>  2386
<211>  4370
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2386
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaag aagcccuaca gcaucggccu ggacaucggg      180

accaacuccg ugggcugggc cguggucacc gacgacuaca aggugcccgc caagaagaug      240

aaggugcucg ggaacacgga caagagccac aucgagaaga accugcuggg cgcccuccug      300

uucgacuccg gcaacaccgc cgaggaccgg cgccugaagc ggaccgcgcg ccggcgcuac      360

acccggcgcc ggaaccgcau ccucuaccug caggagaucu ucagcgagga gauggggaag      420

gucgacgacu ccuucuucca ccggcuggag gacagcuucc ucgugacgga ggacaagcgc      480

ggcgagcggc accccaucuu cgggaaccug gaggaggagg ugaaguacca cgagaacuuc      540

ccgaccaucu accaccuccg ccaguaccug gccgacaacc ccgagaaggu cgaccugcgg      600

cugguguacc ucgcccuggc ccacaucauc aaguuccgcg gccacuuccu gaucgagggg      660

aaguucgaca cccggaacaa cgacgugcag cgccucuucc aggaguuccu ggcggucuac      720

gacaacaccu ucgagaacuc cagccugcag gagcagaacg uccaggugga ggagauccuc      780

acggacaaga ucuccaagag cgccaagaag gaccgggugc ugaagcuguu ccccaacgag      840

aaguccaacg gccgcuucgc cgaguuccuc aagcugaucg ugggcaacca ggccgacuuc      900

aagaagcacu ucgagcugga ggagaaggcc ccgcuccagu ucagcaagga caccuacgag      960

gaggagcugg aggugcugcu cgcgcagauc ggggacaacu acgccgagcu guuccugucc     1020

gccaagaagc ucuacgacag cauccugcug uccggcaucc ucaccgucac cgacgugggg     1080

acgaaggccc cgcugagcgc cuccaugauc cagcgguaca acgagcacca gauggaccug     1140

gcgcagcuca agcaguucau ccgccagaag cugagcgaca aguacaacga gguguucucc     1200

gacgucagca aggacggcua cgccggcuac aucgacggga agaccaacca ggaggccuuc     1260

uacaaguacc ugaagggccu ccugaacaag aucgagggga gcggcuacuu ccuggacaag     1320

aucgagcgcg aggacuuccu ccggaagcag cgcaccuucg acaacggguc caucccccac     1380

cagauccacc uccaggagau gcgggccauc auccgccggc aggccgaguu cuaccccuuc     1440

cuggcggaca accaggaccg caucgagaag cugcugaccu uccggauccc cuacuacgug     1500

ggcccgcugg cccgcggcaa gagcgacuuc gccuggcugu cccgcaagag cgccgacaag     1560

aucacgcccu ggaacuucga cgagaucgug gacaaggagu ccagcgccga ggcguucauc     1620

aaccggauga ccaacuacga ccucuaccug cccaaccaga agguccugcc caagcacucc     1680

cuccuguacg agaaguucac cguguacaac gagcugacca aggucaagua caagacggag     1740

caggggaaga ccgccuucuu cgacgccaac augaagcagg agaucuucga cggcguguuc     1800

aagguguacc ggaaggucac caaggacaag cucauggacu uccuggagaa ggaguucgac     1860

gaguuccgca ucguggaccu gaccgggcuc gacaaggaga acaagguguu caacgccagc     1920

uacggcacgu accacgaccu gugcaagauc cuggacaagg acuuccucga caacuccaag     1980

aacgagaaga uccuggagga caucguccug acccucaccc uguucgagga ccgggagaug     2040

auccgcaagc ggcuggagaa cuacagcgac cuccugacca aggagcaggu caagaagcug     2100

gagcgccggc acuacacggg guggggccgc cucuccgcgg agcugaucca cggcauccgg     2160

aacaaggaga gccgcaagac cauccuggac uaccucaucg acgacgggaa cuccaaccgc     2220

aacuucaugc agcugaucaa cgacgacgcc cugagcuuca aggaggagau cgccaaggcc     2280

caggugaucg gcgagaccga caaccucaac cagguggugu ccgacaucgc cgggagcccg     2340

gcgaucaaga agggcauccu gcagucccug aagaucgugg acgagcucgu caagaucaug     2400

gggcaccagc ccgagaacau cgugguggag auggcccggg agaaccaguu caccaaccag     2460

ggccgccgga acagccagca gcgccugaag ggccugacgg acuccaucaa ggaguucggg     2520

agccagaucc ucaaggagca ccccgucgag aacagccagc ugcagaacga ccggcuguuc     2580

cucuacuacc ugcagaacgg ccgcgacaug uacaccgggg aggagcugga caucgacuac     2640

cucucccagu acgacaucga ccacaucauc ccccaggccu ucaucaagga caacagcauc     2700

gacaaccggg ugcugaccuc cagcaaggag aaccgcggca aguccgacga cgugccgagc     2760

aaggacgucg ugcggaagau gaaguccuac uggagcaagc ugcucuccgc caagcugauc     2820

acccagcgca aguucgacaa ccugacgaag gccgagcggg gcgggcucac cgacgacgac     2880

aaggcgggcu ucaucaagcg ccagcuggug gagacccggc agaucaccaa gcacgucgcc     2940

cgcauccugg acgagcgguu caacaccgag acggacgaga acaacaagaa gauccgccag     3000

gugaagaucg ugacccucaa gagcaaccug gucuccaacu uccggaagga guucgagcug     3060

uacaaggugc gcgagaucaa cgacuaccac cacgcccacg acgccuaccu caacgccgug     3120

aucgggaagg cgcugcuggg cgucuacccc cagcucgagc ccgaguucgu guacggggac     3180

uacccccacu uccacggcca caaggagaac aaggccaccg ccaagaaguu cuucuacagc     3240

aacaucauga acuucuucaa gaaggacgac gugcggaccg acaagaacgg cgagaucauc     3300

uggaagaagg acgagcacau cuccaacauc aagaaggucc ugagcuaccc ccaggugaac     3360

aucgugaaga aggucgagga gcagaccggg ggcuucucca aggagagcau ccugcccaag     3420

gggaacuccg acaagcucau cccccgcaag acgaagaagu ucuacuggga caccaagaag     3480

uacggcgggu ucgacagccc caucguggcc uacagcaucc uggugaucgc ggacaucgag     3540

aagggcaagu ccaagaagcu gaagaccguc aaggcccucg ugggcgugac gaucauggag     3600

aagaugaccu ucgagcggga cccggucgcc uuccuggagc gcaaggggua ccgcaacgug     3660

caggaggaga acaucaucaa gcugcccaag uacagccucu ucaagcugga gaacggccgg     3720

aagcgccugc ucgccuccgc ccgggagcug cagaagggga acgagaucgu gcugcccaac     3780

caccucggca cccugcugua ccacgcgaag aacauccaca aggucgacga gccgaagcac     3840

cucgacuacg uggacaagca caaggacgag uucaaggagc ugcuggacgu ggucagcaac     3900

uucuccaaga aguacacccu cgccgagggg aaccuggaga agaucaagga gcuguacgcc     3960

cagaacaacg gcgaggaccu caaggagcug gccagcuccu ucaucaaccu gcucacguuc     4020

accgccaucg gcgcgcccgc caccuucaag uucuucgaca agaacaucga ccgcaagcgg     4080

uacaccagca cgaccgagau ccugaacgcc acccugaucc accaguccau caccgggcuc     4140

uacgagacgc gcaucgaccu gaacaagcug ggcggggaca agcggcccgc cgccaccaag     4200

aaggcgggcc aggccaagaa gaagaaguaa ggacuagucc cuguucccag agcccacuuu     4260

uuuuucuuuu uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                4370


<210>  2387
<211>  3503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2387
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgcag accaccaacc ugagcuacau ccucggccug      180

gaccugggga ucgccuccgu gggcugggcc guggucgaga ucaacgagaa cgaggacccc      240

aucgggcuca ucgacguggg cgugcggauc uucgagcgcg ccgagguccc caagacgggc      300

gagagccugg cccugucccg gcgccucgcg cggagcaccc gccggcugau ccgccggcgc      360

gcccaccggc ugcuccuggc caagcgcuuc cugaagcggg aggggauccu cuccaccauc      420

gaccuggaga agggccugcc caaccaggcc ugggagcucc gcguggcggg gcuggagcgg      480

cgccugagcg ccaucgagug gggcgccgug cuccugcacc ugaucaagca ccggggguac      540

cucuccaagc gcaagaacga gagccagacc aacaacaagg agcugggcgc ccugcucucc      600

ggcgucgccc agaaccacca gcugcugcag agcgacgacu accggacgcc ggcggagcuc      660

gcccugaaga aguucgccaa ggaggagggg cacauccgca accagcgggg cgccuacacc      720

cacaccuuca accgccugga ccuccuggcc gagcugaacc uccuguucgc gcagcagcac      780

caguucggga acccccacug caaggagcac auccagcagu acaugaccga gcugcucaug      840

uggcagaagc ccgcccuguc cggcgaggcc auccugaaga ugcucggcaa gugcacgcac      900

gagaagaacg aguucaaggc cgccaagcac accuacagcg cggagcgcuu cguguggcug      960

accaagcuga acaaccuccg gauccuggag gacggggccg agcgcgcccu gaacgaggag     1020

gagcggcagc uccugaucaa ccaccccuac gagaagucca agcugaccua cgcccaggug     1080

cgcaagcucc ugggccugag cgagcaggcc aucuucaagc accuccggua cuccaaggag     1140

aacgcggaga gcgccacguu cauggagcuc aaggccuggc acgccauccg caaggcgcug     1200

gagaaccagg ggcugaagga caccuggcag gaccucgcca agaagccgga ccugcuggac     1260

gagaucggca ccgccuucuc ccucuacaag accgacgagg acauccagca guaccugacg     1320

aacaaggucc ccaacagcgu gaucaacgcc cugcucgugu cccugaacuu cgacaaguuc     1380

aucgagcuga gccucaagag ccugcggaag auccugcccc ucauggagca ggggaagcgc     1440

uacgaccagg ccugccggga gaucuacggc caccacuacg gcgaggcgaa ccagaagacc     1500

ucccagcugc ugcccgccau cccggcccag gagauccgca accccgucgu gcuccggacc     1560

cugagccagg cccgcaaggu gaucaacgcc aucauccggc aguacggguc ccccgcgcgc     1620

guccacaucg agaccggccg ggagcugggc aagagcuuca aggagcgccg ggagauccag     1680

aagcagcagg aggacaaccg cacgaagcgg gaguccgccg ugcagaaguu caaggagcug     1740

uucagcgacu ucuccagcga gcccaagucc aaggacaucc ugaaguuccg ccuguacgag     1800

cagcagcacg ggaagugccu cuacagcggg aaggagauca acauccaccg ccugaacgag     1860

aagggcuacg uggagaucga ccacgcccug ccguucuccc ggaccuggga cgacagcuuc     1920

aacaacaagg uccucgugcu ggccuccgag aaccagaaca agggcaacca gacccccuac     1980

gaguggcugc aggggaagau caacagcgag cgcuggaaga acuucguggc ccucguccug     2040

ggcucccagu gcagcgcggc caagaagcag cggcugcuca cccaggugau cgacgacaac     2100

aaguucaucg accgcaaccu gaacgacacg cgguacaucg cccgcuuccu guccaacuac     2160

auccaggaga accuccugcu ggucgggaag aacaagaaga acguguucac ccccaacggc     2220

cagaucaccg cccuccugcg gagccgcugg gggcugauca aggcgcggga gaacaacaac     2280

cgccaccacg cccucgacgc caucgugguc gccugcgcca cccccuccau gcagcagaag     2340

aucacgcggu ucauccgcuu caaggaggug cacccguaca agaucgagaa ccgguacgag     2400

augguggacc aggagagcgg cgagaucauc agcccccacu uccccgagcc cugggcguac     2460

uuccgccagg aggucaacau ccggguguuc gacaaccacc cggacaccgu gcugaaggag     2520

augcugcccg accgccccca ggccaaccac caguucgucc agccccucuu cgugucccgg     2580

gccccgaccc gcaagaugag cggccagggg cacauggaga ccaucaaguc cgccaagcgg     2640

cuggccgagg gcaucagcgu gcugcgcauc ccccucacgc agcugaagcc caaccugcuc     2700

gagaacaugg ucaacaagga gcgggagccg gcgcuguacg ccgggcugaa ggcccgccuc     2760

gccgaguuca accaggaccc cgccaaggcg uucgccaccc ccuucuacaa gcagggcggc     2820

cagcagguca aggccauccg cguggagcag guccagaagu ccggggugcu ggugcgggag     2880

aacaacggcg uggccgacaa cgcgagcauc gugcgcaccg acguguucau caagaacaac     2940

aaguucuucc ugguccccau cuacaccugg caggucgcca aggggauccu cccgaacaag     3000

gccaucgugg cccacaagaa cgaggacgag ugggaggaga uggacgaggg cgccaaguuc     3060

aaguucuccc uguuccccaa cgaccuggug gagcucaaga cgaagaagga guacuucuuc     3120

ggguacuaca ucggccugga ccgggcgacc ggcaacauca gccugaagga gcacgacggg     3180

gagaucucca agggcaagga cgggguguac cgcgugggcg ucaagcucgc ccugagcuuc     3240

gagaaguacc agguggacga gcuggggaag aaccggcaga ucugccgccc ccagcagcgg     3300

cagcccgugc gcaagcggcc ggccgccacc aagaaggccg gccaggcgaa gaagaagaag     3360

uaaggacuag ucccuguucc cagagcccac uuuuuuuucu uuuuuugaaa uaaaauagcc     3420

ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaga auu                                             3503


<210>  2388
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2388
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcggcc gccuucaagc ccaaccccau caacuacauc      180

cugggccucg acaucgggau cgccagcgug ggcugggcca ugguggagau cgacgaggac      240

gagaacccca ucugccugau cgaccugggg guccgggugu ucgagcgcgc ggaggugccg      300

aagaccggcg acucccucgc cauggcccgg cgccuggccc ggagcguccg ccggcugacc      360

cgccggcgcg cgcaccggcu ccugcgcgcc cggcgccugc ucaagcggga gggcgugcug      420

caggccgccg acuucgacga gaacgggcug aucaaguccc uccccaacac gcccuggcag      480

cugcgcgccg cggcccugga ccggaagcuc acgccccugg aguggagcgc cgugcugcuc      540

caccugauca agcaccgcgg cuaccugucc cagcggaaga acgaggggga gaccgccgac      600

aaggagcucg gcgcccugcu gaaggggguc gcggacaacg cccacgcccu ccagaccggc      660

gacuuccgca ccccggccga gcuggcccug aacaaguucg agaaggagag cggccacauc      720

cggaaccagc gcggggacua cucccacacc uucagccgca aggaccucca ggcggagcug      780

auccuccucu ucgagaagca gaaggaguuc ggcaaccccc acguguccgg gggccugaag      840

gagggcaucg agacccugcu caugacccag cggcccgccc ugagcgggga cgccgugcag      900

aagaugcugg gccacugcac guucgagccc gccgagccga aggccgcgaa gaacaccuac      960

accgccgagc gcuucaucug gcucaccaag cugaacaacc ugcggauccu cgagcagggg     1020

uccgagcgcc cccugacgga caccgagcgg gccacccuga uggacgagcc cuaccgcaag     1080

agcaagcuca ccuacgccca ggcgcggaag cugcugggcc ucgaggacac ggccuucuuc     1140

aaggggcugc gcuacggcaa ggacaacgcc gaggccucca cccugaugga gaugaaggcc     1200

uaccacgcga ucagccgggc ccucgagaag gagggccuga aggacaagaa guccccgcug     1260

aaccugagcc ccgagcucca ggacgagauc gggaccgccu ucucccuguu caagaccgac     1320

gaggacauca cgggccgccu gaaggaccgg auccagcccg agauccucga ggcccugcug     1380

aagcacauca gcuucgacaa guucguccag aucucccuca aggcccugcg ccggaucgug     1440

ccccugaugg agcaggggaa gcgcuacgac gaggcgugcg ccgagaucua cggcgaccac     1500

uacgggaaga agaacaccga ggagaagauc uaccugcccc cgauccccgc cgacgagauc     1560

cggaaccccg ugguccugcg cgcccugagc caggcccgga aggugaucaa cggcgugguc     1620

cgccgguacg gcagccccgc gcgcauccac aucgagaccg cccgcgaggu ggggaagucc     1680

uucaaggacc ggaaggagau cgagaagcgc caggaggaga accggaagga ccgcgagaag     1740

gccgccgcga aguuccggga guacuucccg aacuucgugg gcgagcccaa gagcaaggac     1800

auccucaagc ugcgccugua cgagcagcag cacgggaagu gccucuacuc cggcaaggag     1860

aucaaccugg ggcggcugaa cgagaagggc uacgucgaga ucgaccacgc ccuccccuuc     1920

agccgcaccu gggacgacuc cuucaacaac aaggugcugg ugcugggcag cgagaaccag     1980

aacaagggga accagacgcc cuacgaguac uucaacggca aggacaacuc ccgggagugg     2040

caggaguuca aggcccgcgu cgagaccagc cgguucccgc gcuccaagaa gcagcggauc     2100

cuccugcaga aguucgacga ggacggguuc aaggagcgca accugaacga cacccgguac     2160

gugaaccgcu uccucugcca guucgucgcc gaccggaugc gccugaccgg caagggcaag     2220

aagcgggugu ucgccagcaa cgggcagauc acgaaccugc uccgcggcuu cugggggcug     2280

cggaaggugc gcgcggagaa cgaccgccac cacgcccugg acgccgucgu gguggccugc     2340

uccaccgucg ccaugcagca gaagaucacc cgguucgugc gcuacaagga gaugaacgcg     2400

uucgacggca agaccaucga caaggagacg ggggaggugc uccaccagaa gacccacuuc     2460

ccccagcccu gggaguucuu cgcccaggag gucaugaucc ggguguucgg caagccggac     2520

ggcaagcccg aguucgagga ggccgacacc cccgagaagc ugcgcacccu gcucgccgag     2580

aagcugagcu cccggcccga ggccgugcac gaguacguca cgccgcuguu cgugagccgc     2640

gcgcccaacc ggaagauguc cgggcagggc cacauggaga ccgucaagag cgccaagcgc     2700

cucgacgagg ggguguccgu gcugcgggug ccccugaccc agcucaagcu gaaggaccug     2760

gagaagaugg ucaaccgcga gcgggagccc aagcucuacg aggcccugaa ggcccgccug     2820

gaggcgcaca aggacgaccc ggccaaggcc uucgccgagc ccuucuacaa guacgacaag     2880

gccggcaacc ggacccagca ggucaaggcg gugcgcgugg agcaggucca gaagaccggg     2940

gugugggugc ggaaccacaa cggcaucgcc gacaacgcca cgauggugcg cguggacguc     3000

uucgagaagg gcgacaagua cuaccucgug cccaucuaca gcuggcaggu cgccaagggg     3060

auccugcccg accgggccgu ggugcagggc aaggacgagg aggacuggca gcugaucgac     3120

gacuccuuca acuucaaguu cagccuccac ccgaacgacc ugguggaggu caucaccaag     3180

aaggcgcgca uguucgggua cuucgccagc ugccaccggg gcaccgggaa caucaacauc     3240

cgcauccacg accuggacca caagaucggc aagaacggca uccucgaggg gaucggcgug     3300

aagaccgccc uguccuucca gaaguaccag aucgacgagc uggggaagga gauccgcccc     3360

ugccggcuca agaagcgccc gcccgugcgg aagcgccccg ccgccacgaa gaaggcgggc     3420

caggccaaga agaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3480

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  2389
<211>  3587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2389
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaag uaccacgugg gcaucgacgu ggggaccuuc      180

agcgucggcc uggccgccau cgagguggac gacgccggga ugcccaucaa gacccugucc      240

cuggugagcc acauccacga cuccggccug gaccccgacg agaucaagag cgccgucacg      300

cggcucgcgu ccagcggcau cgcccgccgg acccgccggc uguaccgccg gaagcgccgg      360

cgccugcagc agcucgacaa guucauccag cggcaggggu ggcccgugau cgagcuggag      420

gacuacuccg acccgcugua ccccuggaag gugcgcgccg agcucgccgc gagcuacauc      480

gccgacgaga aggagcgggg cgagaagcug uccgucgccc ugcgccacau cgcccggcac      540

cgcggguggc ggaaccccua cgccaaggug agcucccucu accugcccga cggcccgagc      600

gacgcguuca aggccauccg cgaggagauc aagcgggccu ccgggcagcc cgugcccgag      660

accgccaccg ucggccagau ggugacgcug ugcgagcucg gcacccugaa gcugcgcggg      720

gagggcgggg ugcucagcgc ccggcugcag caguccgacu acgcgcgcga gauccaggag      780

aucugccgca ugcaggagau cggccaggag cuguaccgga agaucaucga cgucguguuc      840

gccgccgaga gccccaaggg cuccgccagc ucccgcgugg ggaaggaccc gcuccagccc      900

ggcaagaacc gggcccugaa ggcgagcgac gccuuccagc gcuaccggau cgccgcccug      960

aucgggaacc uccgcguccg gguggacggc gagaagcgca uccugagcgu ggaggagaag     1020

aaccuggucu ucgaccaccu cgugaaccug acccccaaga aggagcccga gugggugacc     1080

aucgccgaga uccuggggau cgaccggggc cagcucaucg gcacggcgac caugaccgac     1140

gacggggagc gcgccggcgc ccggccgccc acccacgaca cgaaccgcuc caucgucaac     1200

agccggaucg cgccccuggu ggacuggugg aagaccgccu ccgcccugga gcagcacgcc     1260

auggucaagg cccucagcaa cgccgaggug gacgacuucg acucccccga gggggcgaag     1320

gugcaggccu ucuucgccga ccuggacgac gacguccacg ccaagcugga cagccuccac     1380

cucccggugg gccgcgccgc guacuccgag gacacccugg ugcggcucac ccgccggaug     1440

cugagcgacg gggucgaccu guacacggcc cgccuccagg aguucggcau cgagcccucc     1500

uggaccccgc ccacgccccg gaucggcgag cccgugggga accccgccgu ggaccgcguc     1560

cugaagaccg ugagccgcug gcuggagucc gccaccaaga ccuggggcgc ccccgagcgg     1620

gugaucaucg agcacguccg cgagggguuc gugaccgaga agcgggcgcg cgagauggac     1680

ggcgacaugc ggcgccgggc cgcccgcaac gccaagcugu uccaggagau gcaggagaag     1740

cugaacgugc aggggaagcc gagccgggcg gaccuguggc gcuaccaguc cguccagcgg     1800

cagaacugcc agugcgccua cugcggcagc cccaucaccu ucuccaacag cgagauggac     1860

cacaucgugc cccgcgccgg ccaggggucc acgaacaccc gggagaaccu cguggccguc     1920

ugccaccgcu gcaaccagag caagggcaac accccguucg ccaucugggc gaagaacacc     1980

uccaucgagg gggugagcgu gaaggaggcc gucgagcgga cgcgccacug ggugaccgac     2040

accggcaugc ggagcaccga cuucaagaag uucacgaagg ccguggucga gcgcuuccag     2100

cgggccacca uggacgagga gaucgacgcc cgcuccaugg agagcguggc guggauggcc     2160

aacgagcugc ggucccgcgu ggcccagcac uucgccagcc acggcaccac cguccgcgug     2220

uaccgggggu cccugacggc cgaggcgcgc cgggccagcg gcaucuccgg gaagcugaag     2280

uucuucgacg gcgugggcaa gagccgccuc gaccggcgcc accacgccau cgacgccgcg     2340

gucaucgccu ucaccuccga cuacguggcc gagacccugg ccgugcggag caaccugaag     2400

cagucccagg cccaccgcca ggaggcgccc caguggcggg aguucaccgg gaaggacgcc     2460

gagcaccgcg ccgccuggcg ggucuggugc cagaagaugg agaagcucag cgcccugcug     2520

accgaggacc uccgcgacga ccggguggug gucaugucca acgugcgccu gcggcugggc     2580

aacgggagcg cgcacaagga gacgaucggc aagcucucca aggugaagcu gagcucccag     2640

cugagcguca gcgacaucga caaggccucc agcgaggccc ucuggugcgc ccugacccgc     2700

gagcccgggu ucgaccccaa ggagggccug ccggccaacc ccgagcggca cauccgcgug     2760

aacgggaccc acguguacgc gggcgacaac aucggccucu uccccgucuc cgccgggagc     2820

aucgcccugc ggggcgggua cgccgagcug ggcuccagcu uccaccacgc ccgcguguac     2880

aagaucaccu ccgggaagaa gcccgcguuc gccaugcucc gcguguacac gaucgaccug     2940

cugccguacc ggaaccagga ccucuucagc gucgagcuga agccccagac cauguccaug     3000

cgccaggccg agaagaagcu gcgggacgcc cucgcgaccg gcaacgccga guaccugggc     3060

uggcuggugg uggacgacga gcucgucgug gacaccagca agaucgccac ggaccaggug     3120

aaggccgucg aggccgagcu ggggaccauc cgccgguggc gcguggacgg cuucuucucc     3180

cccagcaagc ugcggcuccg cccccugcag auguccaagg aggggaucaa gaaggagagc     3240

gcgccggagc uguccaagau caucgaccgg cccggcuggc uccccgccgu caacaagcug     3300

uucagcgacg ggaacgugac cgugguccgc cgggacuccc ugggccgcgu gcggcucgag     3360

agcaccgccc accuccccgu gacguggaag guccagaagc gcccggccgc caccaagaag     3420

gcgggccagg ccaagaagaa gaaguaagga cuagucccug uucccagagc ccacuuuuuu     3480

uucuuuuuuu gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   3587


<210>  2390
<211>  3287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2390
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcggcc cggauccugg ccuucgacau cggcaucagc      180

uccaucgggu gggccuucag cgagaacgac gagcucaagg acugcggcgu gcgcaucuuc      240

accaaggugg agaaccccaa gaccggggag ucccuggccc ugccccggcg ccucgcgcgg      300

agcgcccgca agcggcuggc ccgccggaag gcccgccuga accaccucaa gcaccugauc      360

gcgaacgagu ucaagcugaa cuacgaggac uaccaguccu ucgacgagag ccucgccaag      420

gccuacaagg gcucccugau cagccccuac gagcugcggu uccgcgcccu caacgagcug      480

cuguccaagc aggacuucgc ccgggucauc cuccacaucg cgaagcgccg gggcuacgac      540

gacaucaaga acagcgacga caaggagaag ggggccaucc ugaaggccau caagcagaac      600

gaggagaagc uggccaacua ccaguccgug ggcgaguacc ucuacaagga guacuuccag      660

aaguucaagg agaacagcaa ggaguucacg aacgugcgca acaagaagga guccuacgag      720

cggugcaucg cccagagcuu ccugaaggac gagcugaagc ucaucuucaa gaagcagcgc      780

gaguucgggu ucuccuucag caagaaguuc gaggaggagg uccuguccgu ggcguucuac      840

aagcgggccc ugaaggacuu cagccaccuc gugggcaacu gcuccuucuu caccgacgag      900

aagcgcgccc cgaagaacag cccccuggcc uucauguucg ucgcccugac ccggaucauc      960

aaccuccuga acaaccugaa gaacaccgag gggauccucu acacgaagga cgaccugaac     1020

gcgcugcuca acgaggugcu gaagaacggc acccugaccu acaagcagac caagaagcuc     1080

cugggccuga gcgacgacua cgaguucaag ggggagaagg gcacguacuu caucgaguuc     1140

aagaaguaca aggaguucau caaggcccuc ggggagcaca accuguccca ggacgaccug     1200

aacgagaucg ccaaggacau cacccucauc aaggacgaga ucaagcugaa gaaggcccug     1260

gccaaguacg accucaacca gaaccagauc gacagccugu ccaagcugga guucaaggac     1320

caccucaaca ucagcuucaa ggcgcugaag cuggugacgc cccugaugcu cgagggcaag     1380

aaguacgacg aggccugcaa cgagcugaac cugaaggucg ccaucaacga ggacaagaag     1440

gacuuccucc ccgccuucaa cgagaccuac uacaaggacg aggugaccaa cccggugguc     1500

cugcgcgcga ucaaggagua ccgcaaggug cugaacgccc uccugaagaa guacggcaag     1560

gugcacaaga ucaacaucga gcuggcccgg gaggucggga agaaccacuc ccagcgcgcc     1620

aagaucgaga aggagcagaa cgagaacuac aaggccaaga aggacgcgga gcucgagugc     1680

gagaagcugg gccugaagau caacagcaag aacauccuca agcugcggcu guucaaggag     1740

cagaaggagu ucugcgccua cuccggggag aagaucaaga ucagcgaccu ccaggacgag     1800

aagaugcugg agaucgacca caucuacccc uacucccgca gcuucgacga cuccuacaug     1860

aacaaggugc ugguguucac caagcagaac caggagaagc ucaaccagac ccccuucgag     1920

gccuucggca acgacagcgc caaguggcag aagaucgagg uccuggccaa gaaccugccc     1980

accaagaagc agaagcggau ccucgacaag aacuacaagg acaaggagca gaagaacuuc     2040

aaggaccgca accugaacga cacgcgguac aucgcgcgcc uggugcucaa cuacaccaag     2100

gacuaccugg acuuccugcc gcucuccgac gacgagaaca ccaagcugaa cgacacccag     2160

aaggggagca aggugcacgu cgaggccaag uccggcaugc ugacgagcgc ccuccggcac     2220

accuggggcu ucuccgccaa ggaccgcaac aaccaccucc accacgccau cgacgcggug     2280

aucaucgccu acgccaacaa cagcaucguc aaggccuucu ccgacuucaa gaaggagcag     2340

gagagcaaca gcgcggagcu guacgccaag aagaucuccg agcuggacua caagaacaag     2400

cggaaguucu ucgagcccuu cagcggguuc cgccagaagg ugcuggacaa gaucgacgag     2460

aucuucgugu ccaagcccga gcggaagaag cccagcggcg cccugcacga ggagaccuuc     2520

cgcaaggagg aggaguucua ccaguccuac gggggcaagg aggggguccu caaggcccug     2580

gagcugggca agauccggaa ggugaacggc aagaucguga agaacgggga cauguuccgc     2640

gucgacaucu ucaagcacaa gaagaccaac aaguucuacg ccgugccgau cuacacgaug     2700

gacuucgcgc ucaaggugcu gcccaacaag gccgucgccc ggagcaagaa gggcgagauc     2760

aaggacugga uccugaugga cgagaacuac gaguucugcu ucucccucua caaggacagc     2820

cugauccuga uccagaccaa ggacaugcag gagcccgagu ucguguacua caacgccuuc     2880

accuccagca ccgugucccu caucgucagc aagcacgaca acaaguucga gacgcugucc     2940

aagaaccaga agauccuguu caagaacgcc aacgagaagg aggugaucgc caagagcauc     3000

gggauccaga accucaaggu guucgagaag uacaucgucu ccgcgcuggg cgaggugacc     3060

aaggccgagu uccgccagcg ggaggacuuc aagaagaagc gccccgccgc caccaagaag     3120

gcggggcagg ccaagaagaa gaaguaagga cuagucccug uucccagagc ccacuuuuuu     3180

uucuuuuuuu gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   3287


<210>  2391
<211>  3698
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2391
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgagc gaccuggugc ucggccugga caucgggauc      180

ggcuccgugg gggucggcau ccugaacaag gugaccggcg agaucaucca caagaacagc      240

cggaucuucc ccgccgccca ggccgagaac aaccucgugc gccggaccaa ccgccagggg      300

cggcgccugg cccggcgcaa gaagcaccgg cgcguccggc ugaaccgccu cuucgaggag      360

uccggccuga ucacggacuu caccaagauc agcaucaacc ugaaccccua ccagcuccgg      420

gugaaggggc ugaccgacga gcuguccaac gaggagcugu ucaucgcgcu gaagaacaug      480

gugaagcacc gcggcaucag cuaccuggac gacgccuccg acgacgggaa cagcuccguc      540

ggcgacuacg cccagaucgu gaaggagaac agcaagcagc ucgagaccaa gacgcccggc      600

cagauccagc uggagcggua ccagaccuac gggcagcugc gcggcgacuu caccguggag      660

aaggacggga agaagcaccg gcucaucaac gucuucccga ccuccgccua ccgcagcgag      720

gcgcugcgga uccugcagac gcagcaggag uucaaccccc agaucaccga cgaguucauc      780

aaccgcuacc ucgagauccu gaccggcaag cggaaguacu accacggccc cgggaacgag      840

aagucccgca ccgacuacgg ccgcuaccgg acgagcgggg agacccugga caacaucuuc      900

ggcauccuca ucgggaagug caccuucuac cccgacgagu uccgcgccgc caaggccucc      960

uacaccgccc aggaguucaa ccugcugaac gaccucaaca accugacggu gccgaccgag     1020

accaagaagc ugagcaagga gcagaagaac cagaucauca acuacgugaa gaacgagaag     1080

gcgaugggcc ccgccaagcu guucaaguac aucgccaagc uccuguccug cgacgucgcc     1140

gacaucaagg gcuaccggau cgacaagagc gggaaggccg agauccacac cuucgaggcg     1200

uaccgcaaga ugaagacgcu cgagacccug gacaucgagc agauggaccg ggagacccug     1260

gacaagcucg ccuacgugcu gacccugaac acggagcgcg agggcaucca ggaggcccuc     1320

gagcacgagu ucgccgacgg guccuucagc cagaagcagg ucgacgagcu ggugcaguuc     1380

cggaaggcca acagcuccau cuucggcaag ggguggcaca acuucagcgu gaagcugaug     1440

auggagcuca uccccgagcu guacgagacc uccgaggagc agaugaccau ccugacccgc     1500

cucggcaagc agaagacgac cagcuccagc aacaagacca aguacaucga cgagaagcug     1560

cugaccgagg agaucuacaa ccccgugguc gcgaaguccg ugcggcaggc caucaagauc     1620

gugaacgccg ccaucaagga guacggcgac uucgacaaca ucgucaucga gauggcccgc     1680

gagacgaacg aggacgacga gaagaaggcg auccagaaga uccagaaggc caacaaggac     1740

gagaaggacg ccgccaugcu caaggcggcc aaccaguaca acgggaaggc cgagcugccg     1800

cacagcgugu uccacggcca caagcagcug gccaccaaga uccggcugug gcaccagcag     1860

ggggagcgcu gccucuacac cggcaagacc aucuccaucc acgaccugau caacaacagc     1920

aaccaguucg aggucgacca cauccugccc cucuccauca cguucgacga cagccuggcc     1980

aacaaggugc ugguguacgc gaccgccaac caggagaagg ggcagcggac ccccuaccag     2040

gcccucgacu ccauggacga cgccuggagc uuccgcgagc ugaaggccuu cguccgggag     2100

uccaagaccc ugagcaacaa gaagaaggag uaccuccuga ccgaggagga caucuccaag     2160

uucgacgugc gcaagaaguu caucgagcgg aaccuggugg acacgcgcua cgcgagccgg     2220

gucgugcuca acgcccugca ggagcacuuc cgcgcccaca agaucgacac caaggugucc     2280

gucgugcgcg gccaguucac cagccagcug cggcgccacu ggggcaucga gaagacccgg     2340

gacacguacc accaccacgc cguggacgcc cucaucaucg cggccagcuc ccagcugaac     2400

cuguggaaga agcagaagaa cacccucguc agcuacuccg aggaccagcu gcuggacauc     2460

gagaccgggg agcucaucag cgacgacgag uacaaggagu ccguguucaa ggcccccuac     2520

cagcacuucg uggacacccu caagagcaag gaguucgagg acuccauccu guucagcuac     2580

caggucgacu ccaaguucaa ccgcaagauc agcgacgcca cgaucuacgc gacccggcag     2640

gccaaggugg gcaaggacaa ggccgacgag accuacgugc ucgggaagau caaggacauc     2700

uacacccagg acggcuacga cgccuucaug aagaucuaca agaaggacaa guccaaguuc     2760

cugauguacc gccacgaccc gcagacguuc gagaagguca ucgagcccau ccuggagaac     2820

uaccccaaca agcagaucaa cgagaagggc aaggaggugc ccugcaaccc guuccucaag     2880

uacaaggagg agcacgggua cauccggaag uacagcaaga agggcaacgg gcccgagauc     2940

aagucccuga aguacuacga cagcaagcug ggcaaccaca ucgacaucac ccccaaggac     3000

uccaacaaca aggugguccu ccagagcgug ucccccuggc gcgccgacgu guacuucaac     3060

aagaccaccg ggaaguacga gauccugggc cugaaguacg cggaccucca guucgagaag     3120

ggcacgggga ccuacaagau cagccaggag aaguacaacg acaucaagaa gaaggagggc     3180

gucgacagcg acuccgaguu caaguucacc cuguacaaga acgaccugcu ccuggugaag     3240

gacaccgaga cgaaggagca gcagcuguuc cgguuccuca gccgcaccau gccgaagcag     3300

aagcacuacg uggagcugaa gcccuacgac aagcagaagu ucgagggcgg ggaggcccug     3360

aucaaggucc ucgggaacgu ggccaacucc ggccagugca agaagggccu gggcaagagc     3420

aacaucucca ucuacaaggu gcggaccgac guccugggga accagcacau caucaagaac     3480

gagggggaca agcccaagcu cgacuucaag cgccccgccg ccaccaagaa ggcgggccag     3540

gccaagaaga agaaguaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu     3600

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                             3698


<210>  2392
<211>  4256
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2392
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgacc caguucgagg gcuucaccaa ccuguaccag      180

gugagcaaga cgcuccgguu cgagcugauc ccccagggga agacccugaa gcacauccag      240

gagcagggcu ucaucgagga ggacaaggcc cgcaacgacc acuacaagga gcucaagccc      300

aucaucgacc ggaucuacaa gaccuacgcc gaccagugcc ugcagcuggu gcagcucgac      360

ugggagaacc uguccgccgc caucgacagc uaccgcaagg agaagaccga ggagacgcgg      420

aacgcgcuga ucgaggagca ggccaccuac cgcaacgcca uccacgacua cuucaucggg      480

cggaccgaca accucaccga cgccaucaac aagcgccacg cggagaucua caagggccug      540

uucaaggccg agcuguucaa cggcaagguc cucaagcagc uggggacggu gaccaccacc      600

gagcacgaga acgcccugcu ccgguccuuc gacaaguuca cgaccuacuu cagcggcuuc      660

uacgagaacc gcaagaacgu guucuccgcc gaggacauca gcaccgccau cccccaccgg      720

aucguccagg acaacuuccc gaaguucaag gagaacugcc acaucuucac ccgccugauc      780

acggcggugc ccucccugcg ggagcacuuc gagaacguga agaaggccau cgggaucuuc      840

gucagcaccu ccaucgagga gguguucagc uuccccuucu acaaccagcu ccugacccag      900

acccagaucg accuguacaa ccagcuccug ggcgggaucu cccgcgaggc cggcacggag      960

aagaucaagg gccugaacga ggugcucaac cuggccaucc agaagaacga cgagaccgcc     1020

cacaucaucg cgagccugcc ccaccgguuc aucccccucu ucaagcagau ccuguccgac     1080

cgcaacaccc ugagcuucau ccucgaggag uucaaguccg acgaggaggu cauccagagc     1140

uucugcaagu acaagacccu gcugcggaac gagaacgugc ucgagacggc cgaggcccug     1200

uucaacgagc ugaacuccau cgaccucacc cacaucuuca ucagccacaa gaagcuggag     1260

accaucucca gcgcccugug cgaccacugg gacacccucc gcaacgcccu guacgagcgg     1320

cgcaucagcg agcugacggg gaagaucacc aaguccgcga aggagaaggu gcagcggagc     1380

cucaagcacg aggacaucaa ccugcaggag aucaucuccg ccgccggcaa ggagcugagc     1440

gaggccuuca agcagaagac cuccgagauc cucagccacg cccacgcggc ccuggaccag     1500

ccgcugccca ccacgcucaa gaagcaggag gagaaggaga uccugaaguc ccagcuggac     1560

agccugcucg ggcuguacca ccuccucgac ugguucgccg ucgacgaguc caacgaggug     1620

gaccccgagu ucagcgcccg ccugaccggc aucaagcugg agauggagcc gucccucagc     1680

uucuacaaca aggcgcgcaa cuacgccacc aagaagcccu acuccgugga gaaguucaag     1740

cugaacuucc agaugcccac ccuggccagc ggcugggacg ucaacaagga gaagaacaac     1800

ggggccaucc ucuucgugaa gaacggccug uacuaccugg ggaucaugcc caagcagaag     1860

ggccgguaca aggcccucuc cuucgagccg acggagaaga ccagcgaggg guucgacaag     1920

auguacuacg acuacuuccc cgacgcggcc aagaugaucc ccaagugcuc cacccagcug     1980

aaggccguga ccgcccacuu ccagacccac acgaccccca uccugcucag caacaacuuc     2040

aucgagccgc uggagaucac caaggagauc uacgaccuga acaaccccga gaaggagccc     2100

aagaaguucc agaccgccua cgcgaagaag acgggcgacc agaagggcua ccgcgaggcc     2160

cucugcaagu ggaucgacuu cacccgggac uuccugucca aguacaccaa gaccacgagc     2220

aucgaccuga gcucccuccg ccccagcucc caguacaagg accuggggga guacuacgcc     2280

gagcugaacc cgcuccugua ccacaucagc uuccagcgga ucgccgagaa ggagaucaug     2340

gacgccgucg agaccggcaa gcuguaccuc uuccagaucu acaacaagga cuucgcgaag     2400

gggcaccacg gcaagcccaa ccugcacacc cuguacugga ccggccucuu cucccccgag     2460

aaccuggcca agacgagcau caagcugaac gggcaggccg agcuguucua ccgccccaag     2520

ucccggauga agcgcauggc ccaccggcug ggcgagaaga ugcugaacaa gaagcucaag     2580

gaccagaaga ccccgauccc cgacacccug uaccaggagc uguacgacua cgugaaccac     2640

cgccucagcc acgaccuguc cgacgaggcg cgggcccugc uccccaacgu gaucaccaag     2700

gaggucagcc acgagaucau caaggaccgc cgguucacgu ccgacaaguu cuucuuccac     2760

gugccgauca cccugaacua ccaggccgcc aacagccccu ccaaguucaa ccagcgcguc     2820

aacgccuacc ugaaggagca ccccgagacc cccaucaucg ggaucgaccg gggcgagcgc     2880

aaccucaucu acaucaccgu gaucgacagc acggggaaga uccuggagca gcggucccug     2940

aacaccaucc agcaguucga cuaccagaag aagcucgaca accgcgagaa ggagcgggug     3000

gcggcccgcc aggccuggag cgucgugggc accaucaagg accugaagca gggguaccug     3060

ucccagguga uccacgagau cgucgaccuc augauccacu accaggccgu ggugguccug     3120

gagaaccuga acuucggcuu caagagcaag cgcaccggca ucgccgagaa ggcgguguac     3180

cagcaguucg agaagaugcu caucgacaag cugaacugcc uggugcucaa ggacuacccg     3240

gccgagaagg ucgggggcgu gcugaacccc uaccagcuga cggaccaguu caccagcuuc     3300

gccaagaugg ggacccaguc cggcuuccuc uucuacgugc ccgcccccua caccagcaag     3360

aucgacccgc ugacgggcuu cgucgacccc uucgugugga agaccaucaa gaaccacgag     3420

ucccggaagc acuuccugga gggguucgac uuccuccacu acgacgugaa gaccggcgac     3480

uucauccugc acuucaagau gaaccgcaac cugagcuucc agcgggggcu ccccggcuuc     3540

augcccgccu gggacaucgu cuucgagaag aacgagaccc aguucgacgc gaaggggacg     3600

ccguucaucg ccggcaagcg caucgugccc gugaucgaga accaccgguu caccggccgc     3660

uaccgggacc uguaccccgc caacgagcug aucgcccucc uggaggagaa ggggaucguc     3720

uuccgcgacg gcuccaacau ccugcccaag cuccuggaga acgacgacag ccacgcgauc     3780

gacaccaugg uggcccugau ccgguccgug cuccagaugc gcaacagcaa cgccgccacc     3840

ggggaggacu acaucaacuc cccgguccgg gaccugaacg gcgugugcuu cgacagccgc     3900

uuccagaacc ccgaguggcc cauggacgcc gacgcgaacg gggccuacca caucgcccug     3960

aagggccagc uccugcugaa ccaccucaag gaguccaagg accugaagcu gcagaacggc     4020

aucagcaacc aggacuggcu cgccuacauc caggagcugc ggaacaagcg ccccgccgcg     4080

acgaagaagg ccgggcaggc caagaagaag aaguaaggac uagucccugu ucccagagcc     4140

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         4256


<210>  2393
<211>  4235
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2393
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgagc aucuaccagg aguucgugaa caaguacucc      180

cugagcaaga cccuccgguu cgagcugauc ccccagggca agacccugga gaacaucaag      240

gcccgcgggc ucauccugga cgacgagaag cgggccaagg acuacaagaa ggccaagcag      300

aucaucgaca aguaccacca guucuucauc gaggagaucc uguccagcgu gugcaucucc      360

gaggaccucc ugcagaacua cagcgacguc uacuucaagc ugaagaaguc cgacgacgac      420

aaccuccaga aggacuucaa gagcgccaag gacacgauca agaagcagau cuccgaguac      480

aucaaggaca gcgagaaguu caagaaccug uucaaccaga accugaucga cgcgaagaag      540

ggccaggagu ccgaccucau ccuguggcug aagcagagca aggacaacgg gaucgagcug      600

uucaaggcca acuccgacau caccgacauc gacgaggccc ucgagaucau caagagcuuc      660

aagggcugga ccaccuacuu caagggcuuc cacgagaacc gcaagaacgu guacuccagc      720

aacgacaucc ccacguccau caucuaccgg aucguggacg acaaccugcc caaguuccuc      780

gagaacaagg ccaaguacga gagccugaag gacaaggcgc cggaggccau caacuacgag      840

cagaucaaga aggaccuggc cgaggagcuc accuucgaca ucgacuacaa gaccuccgag      900

gucaaccagc gcguguucag ccuggacgag guguucgaga ucgccaacuu caacaacuac      960

cugaaccaga gcgggaucac caaguucaac acgaucaucg gcgggaaguu cgucaacggc     1020

gagaacacca agcggaaggg gaucaacgag uacaucaacc ucuacuccca gcagaucaac     1080

gacaagaccc ugaagaagua caagaugagc gugcuguuca agcagauccu cuccgacacc     1140

gagagcaagu ccuucgugau cgacaagcug gaggacgaca gcgacgucgu gacgaccaug     1200

caguccuucu acgagcagau cgccgcguuc aagaccgugg aggagaaguc caucaaggag     1260

acccuguccc uccuguucga cgaccugaag gcccagaagc ucgaccugag caagaucuac     1320

uucaagaacg acaagucccu gacggaccuc agccagcagg ucuucgacga cuacuccgug     1380

aucggcaccg ccgugcugga guacaucacc cagcagaucg cccccaagaa ccuggacaac     1440

cccagcaaga aggagcagga gcucaucgcc aagaagaccg agaaggcgaa guaccugucc     1500

cuggagacga ucaagcucgc ccuggaggag uucaacaagc accgcgacau cgacaagcag     1560

ugccgguucg aggagauccu ggccaacuuc gccgccaucc ccaugaucuu cgacgagauc     1620

gcgcagaaca aggacaaccu cgcccagauc agcaucaagu accagaacca gggcaagaag     1680

gaccugcugc aggccuccgc cgaggacgac gucaaggcca ucaaggaccu ccuggaccag     1740

accaacaacc ugcugcacaa gcucaagauc uuccacauca gccaguccga ggacaaggcg     1800

aacauccugg acaaggacga gcacuucuac cugguguucg aggagugcua cuucgagcuc     1860

gccaacaucg ugccgcugua caacaagauc cgcaacuaca ucacccagaa gcccuacagc     1920

gacgagaagu ucaagcugaa cuucgagaac agcacccucg ccaacgggug ggacaagaac     1980

aaggagcccg acaacacggc cauccuguuc aucaaggacg acaaguacua ccugggcguc     2040

augaacaaga agaacaacaa gaucuucgac gacaaggcga ucaaggagaa caagggggag     2100

ggcuacaaga agaucgugua caagcuccug cccggcgcca acaagaugcu gccgaaggug     2160

uucuucuccg ccaagagcau caaguucuac aaccccuccg aggacauccu ccggauccgc     2220

aaccacagca cccacaccaa gaacgggagc ccccagaagg gcuacgagaa guucgaguuc     2280

aacaucgagg acugccggaa guucaucgac uucuacaagc agagcaucuc caagcacccc     2340

gaguggaagg acuucggguu ccgcuucagc gacacccagc gguacaacuc caucgacgag     2400

uucuaccgcg aggucgagaa ccagggcuac aagcugacgu ucgagaacau cagcgagucc     2460

uacaucgaca gcguggucaa ccaggggaag cuguaccucu uccagaucua caacaaggac     2520

uucuccgccu acagcaaggg ccggccgaac cugcacaccc uguacuggaa ggcccucuuc     2580

gacgagcgca accugcagga cgugguguac aagcugaacg gcgaggcgga gcuguucuac     2640

cggaagcagu ccauccccaa gaagaucacc caccccgcca aggaggccau cgccaacaag     2700

aacaaggaca accccaagaa ggagagcguc uucgaguacg accucaucaa ggacaagcgc     2760

uucaccgagg acaaguucuu cuuccacugc ccgaucacga ucaacuucaa guccagcggg     2820

gccaacaagu ucaacgacga gaucaaccug cuccugaagg agaaggcgaa cgacgugcac     2880

auccugagca ucgaccgggg cgagcgccac cucgccuacu acacccuggu ggacgggaag     2940

ggcaacauca ucaagcagga caccuucaac aucaucggga acgaccgcau gaagaccaac     3000

uaccacgaca agcuggccgc caucgagaag gaccgggacu ccgcccgcaa ggacuggaag     3060

aagaucaaca acaucaagga gaugaaggag ggcuaccuca gccaggucgu gcacgagauc     3120

gcgaagcugg ugaucgagua caacgccauc gucguguucg aggaccugaa cuucggcuuc     3180

aagcgggggc gcuucaaggu ggagaagcag gucuaccaga agcucgagaa gaugcugauc     3240

gagaagcuga acuaccucgu guucaaggac aacgaguucg acaagacggg cggggugcug     3300

cgggccuacc agcugaccgc ccccuucgag accuucaaga agaugggcaa gcagaccggg     3360

aucaucuacu acguccccgc gggcuucacc uccaagaucu gccccgugac gggcuucgug     3420

aaccagcucu acccgaagua cgagagcguc uccaagagcc aggaguucuu cuccaaguuc     3480

gacaagaucu gcuacaaccu ggacaagggg uacuucgagu ucagcuucga cuacaagaac     3540

uucggcgaca aggccgccaa ggggaagugg accaucgccu ccuucggcag ccgccugauc     3600

aacuuccgga acuccgacaa gaaccacaac ugggacaccc gcgaggugua ccccaccaag     3660

gagcucgaga agcugcugaa ggacuacagc aucgaguacg gccacgggga gugcaucaag     3720

gccgcgaucu gcggcgaguc cgacaagaag uucuucgcca agcucacgag cgugcugaac     3780

accauccugc agaugcggaa cuccaagacc gggaccgagc ucgacuaccu gaucagcccc     3840

gucgccgacg ugaacggcaa cuucuucgac ucccgccagg cccccaagaa caugccgcag     3900

gacgccgacg cgaacggggc cuaccacauc ggccugaagg gccucaugcu gcuggggcgg     3960

aucaagaaca accaggaggg caagaagcuc aaccugguga ucaagaacga ggaguacuuc     4020

gaguucgucc agaaccgcaa caacaagcgg cccgccgcca cgaagaaggc cgggcaggcg     4080

aagaagaaga aguaaggacu agucccuguu cccagagccc acuuuuuuuu cuuuuuuuga     4140

aauaaaauag ccugucuuuc agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                4235


<210>  2394
<211>  4420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2394
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgga caagaaguac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccgugaucac cgacgaguac aaggucccca gcaagaaguu caaggugcug gggaacacgg      240

accggcacuc caucaagaag aaccugaucg gcgcccugcu guucgacagc ggcgagaccg      300

ccgaggccac ccgccugaag cggaccgcgc gccggcgcua cacgcggcgc aagaaccgga      360

ucugcuaccu gcaggagauc uucuccaacg agauggccaa gguggacgac agcuucuucc      420

accgccucga ggaguccuuc cuggucgagg aggacaagaa gcacgagcgg caccccaucu      480

ucgggaacau cguggacgag guggccuacc acgagaagua ccccaccauc uaccaccucc      540

gcaagaagcu cgucgacagc accgacaagg ccgaccugcg gcugaucuac cucgcgcugg      600

cccacaugau caaguuccgc ggccacuucc ugaucgaggg ggaccucaac ccggacaacu      660

ccgacgugga caagcuguuc auccagcugg ugcagaccua caaccagcug uucgaggaga      720

accccaucaa cgccagcggc gucgacgcca aggccauccu guccgcgcgg cugagcaagu      780

cccgccggcu cgagaaccug aucgcccagc ugcccgggga gaagaagaac ggccucuucg      840

gcaaccugau cgcccugagc cucgggcuga cgcccaacuu caaguccaac uucgaccugg      900

ccgaggacgc caagcuccag cugagcaagg acaccuacga cgacgaccug gacaaccucc      960

uggcgcagau cggcgaccag uacgccgacc uguuccucgc cgccaagaac cuguccgacg     1020

ccauccugcu cagcgacauc cugcgcguga acaccgagau caccaaggcg ccgcuguccg     1080

ccagcaugau caagcgguac gacgagcacc accaggaccu cacgcugcug aaggcccucg     1140

ugcgccagca gcugcccgag aaguacaagg agaucuucuu cgaccagagc aagaacgggu     1200

acgccggcua caucgacggc ggggccuccc aggaggaguu cuacaaguuc aucaagccca     1260

uccuggagaa gauggacggc accgaggagc uccuggucaa gcugaaccgg gaggaccugc     1320

uccgcaagca gcgcaccuuc gacaacggga gcauccccca ccagauccac cugggcgagc     1380

ugcacgcgau ccuccggcgc caggaggacu ucuacccguu ccugaaggac aaccgggaga     1440

agaucgagaa gauccugacc uuccgcaucc ccuacuacgu ggggccccuc gcccggggca     1500

acucccgcuu cgccuggaug acgcgcaaga gcgaggagac caucaccccc uggaacuucg     1560

aggagguggu cgacaagggc gccuccgcgc agagcuucau cgagcggaug accaacuucg     1620

acaagaaccu gccgaacgag aaggugcugc ccaagcacuc ccuccuguac gaguacuuca     1680

cgguguacaa cgagcugacc aaggucaagu acgugaccga ggggaugcgg aagcccgccu     1740

uccucagcgg cgagcagaag aaggccaucg uggaccugcu guucaagacc aaccgcaagg     1800

ucacggugaa gcagcucaag gaggacuacu ucaagaagau cgagugcuuc gacuccgucg     1860

agaucagcgg gguggaggac cgguucaacg ccucccuggg caccuaccac gaccugcuca     1920

agaucaucaa ggacaaggac uuccuggaca acgaggagaa cgaggacauc cuggaggaca     1980

ucgugcucac ccugacccug uucgaggacc gcgagaugau cgaggagcgg cucaagacgu     2040

acgcccaccu guucgacgac aaggucauga agcagcugaa gcgccggcgc uacaccgggu     2100

ggggccggcu cagccgcaag cugaucaacg gcauccggga caagcagucc gggaagacca     2160

uccuggacuu ccucaagagc gacggcuucg cgaaccgcaa cuucaugcag cugauccacg     2220

acgacucccu gaccuucaag gaggacaucc agaaggccca ggugagcggg cagggcgacu     2280

cccuccacga gcacaucgcc aaccuggccg ggagccccgc caucaagaag ggcauccugc     2340

agacggugaa ggucguggac gagcucguga aggucauggg ccgccacaag ccggagaaca     2400

ucgugaucga gauggcgcgg gagaaccaga ccacccagaa ggggcagaag aacucccgcg     2460

agcggaugaa gcgcaucgag gagggcauca aggagcuggg gagccagauc cugaaggagc     2520

accccgugga gaacacccag cuccagaacg agaagcugua ccuguacuac cuccagaacg     2580

gccgggacau guacgucgac caggagcugg acaucaaccg ccugagcgac uacgacgugg     2640

accacaucgu gccccagucc uuccucaagg acgacagcau cgacaacaag guccugacgc     2700

gguccgacaa gaaccgcggc aagagcgaca acgugcccuc cgaggaggug gucaagaaga     2760

ugaagaacua cuggcggcag cugcucaacg ccaagcugau cacccagcgc aaguucgaca     2820

accugaccaa ggccgagcgc gggggccuca gcgagcugga caaggccggg uucaucaagc     2880

ggcagcuggu ggagacccgg cagaucacca agcacguggc ccagauccuc gacucccgca     2940

ugaacacgaa guacgacgag aacgacaagc ugauccggga ggucaaggug aucacccuca     3000

agagcaagcu cguguccgac uuccgcaagg acuuccaguu cuacaagguc cgggagauca     3060

acaacuacca ccacgcgcac gacgccuacc ugaacgccgu ggugggcacc gcccugauca     3120

agaaguaccc gaagcucgag agcgaguucg ucuacgggga cuacaaggug uacgacgugc     3180

gcaagaugau cgcgaagucc gagcaggaga ucggcaaggc caccgccaag uacuucuucu     3240

acagcaacau caugaacuuc uucaagacgg agaucacccu ggccaacggc gagauccgga     3300

agcgcccccu gaucgagacc aacggggaga ccggcgagau cgucugggac aaggggcggg     3360

acuucgccac ggugcgcaag gugcucucca ugccccaggu caacaucgug aagaagaccg     3420

aggugcagac cggcggguuc agcaaggagu ccauccugcc caagcgcaac agcgacaagc     3480

ugaucgcgcg gaagaaggac ugggacccga agaaguacgg cggcuucgac ucccccaccg     3540

ucgccuacag cgugcucgug gucgccaagg uggagaaggg caagagcaag aagcugaagu     3600

ccgugaagga gcugcucggg aucacgauca uggagcgcag cuccuucgag aagaacccca     3660

ucgacuuccu ggaggccaag ggguacaagg aggucaagaa ggaccugauc aucaagcucc     3720

cgaaguacag ccuguucgag cuggagaacg gccggaagcg caugcucgcc uccgcggggg     3780

agcugcagaa gggcaacgag cuggcccucc ccagcaagua cgugaacuuc cuguaccugg     3840

ccucccacua cgagaagcuc aagggcagcc ccgaggacaa cgagcagaag cagcuguucg     3900

uggagcagca caagcacuac cuggacgaga ucaucgagca gaucuccgag uucagcaagc     3960

gggucauccu cgccgacgcc aaccuggaca aggugcuguc cgcguacaac aagcaccgcg     4020

acaagcccau ccgggagcag gccgagaaca ucauccaccu cuucacccug accaaccugg     4080

gggccccggc cgcguucaag uacuucgaca ccacgaucga ccgcaagcgg uacaccagca     4140

ccaaggaggu gcucgacgcc acccugaucc accaguccau cacgggccug uacgagaccc     4200

gcaucgaccu cagccagcuc gggggcgaca agcggcccgc cgccaccaag aaggccgggc     4260

aggcgaagaa gaagaaguaa ggacuagucc cuguucccag agcccacuuu uuuuucuuuu     4320

uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                           4420


<210>  2395
<211>  5203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2395
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa cuucaagauc cugcccaucg ccaucgaccu cggcgugaag aacaccgggg      180

uguucagcgc cuucuaccag aagggcaccu cccuggagcg gcuggacaac aagaacggga      240

aggucuacga gcucagcaag gacuccuaca cgcugcugau gaacaaccgc accgcccggc      300

gccaccagcg gcgcggcauc gaccggaagc agcucgugaa gcgccuguuc aagcugaucu      360

ggaccgagca gcucaaccug gagugggaca aggacaccca gcaggccauc agcuuccugu      420

ucaaccggcg cggcuucucc uucaucacgg acggguacag ccccgaguac cucaacaucg      480

ugcccgagca ggucaaggcg auccugaugg acaucuucga cgacuacaac ggcgaggacg      540

accuggacuc cuaccucaag cuggccaccg agcaggagag caagaucucc gagaucuaca      600

acaagcugau gcagaagauc cucgaguuca agcugaugaa gcugugcacc gacaucaagg      660

acgacaaggu gagcaccaag acgcucaagg agaucaccuc cuacgaguuc gagcugcugg      720

ccgacuaccu cgccaacuac agcgaguccc ugaagaccca gaaguucagc uacaccgaca      780

agcaggggaa ccugaaggag cucuccuacu accaccacga caaguacaac auccaggagu      840

uccugaagcg gcacgcgacg aucaacgacc gcauccugga cacccuccug accgacgacc      900

uggacaucug gaacuucaac uucgagaagu ucgacuucga caagaacgag gagaagcucc      960

agaaccagga ggacaaggac cacauccagg cccaccucca ccacuucgug uucgccguca     1020

acaagaucaa gagcgagaug gccuccggcg ggcggcaccg cagccaguac uuccaggaga     1080

ucaccaacgu gcuggacgag aacaaccacc aggagggcua ccugaagaac uucugcgaga     1140

accugcacaa caagaaguac uccaaccuga gcgugaagaa ccucgucaac cugaucggca     1200

accugagcaa ccucgagcug aagccgcugc ggaaguacuu caacgacaag auccacgcca     1260

aggcggacca cugggacgag cagaaguuca cggagaccua cugccacugg auccucgggg     1320

aguggcgcgu gggcgugaag gaccaggaca agaaggacgg ggccaaguac uccuacaagg     1380

accugugcaa cgagcugaag cagaagguca ccaaggccgg ccucguggac uuccugcugg     1440

agcuggaccc cugccggacc aucccgcccu accucgacaa caacaaccgc aagccgccca     1500

agugccagag ccugauccug aaccccaagu uccucgacaa ccaguacccc aacuggcagc     1560

aguaccugca ggagcugaag aagcuccagu ccauccagaa cuaccuggac agcuucgaga     1620

cggaccugaa ggugcucaag uccagcaagg accagcccua cuucgucgag uacaagucca     1680

gcaaccagca gaucgccucc ggccagcggg acuacaagga ccuggacgcc cgcauccugc     1740

aguucaucuu cgaccgggug aaggcgagcg acgagcuccu gcugaacgag aucuacuucc     1800

aggccaagaa gcucaagcag aaggccucca gcgagcugga gaagcuggag uccagcaaga     1860

agcucgacga ggugaucgcc aacucccagc ugagccagau ccugaagucc cagcacacca     1920

acgggaucuu cgagcagggc accuuccucc accuggucug caaguacuac aagcagcgcc     1980

agcgcgcccg ggacagccgc cuguacauca ugcccgagua ccgguacgac aagaagcucc     2040

acaaguacaa caacaccggg cgcuucgacg acgacaacca gcugcugacg uacugcaacc     2100

acaagccccg gcagaagcgc uaccagcucc ugaacgaccu ggcgggcgug cuccaggugu     2160

cccccaacuu ccugaaggac aagaucggga gcgacgacga ccuguucauc agcaaguggc     2220

ucgucgagca cauccggggc uucaagaagg ccugcgagga cucccugaag auccagaagg     2280

acaaccgcgg ccugcucaac cacaagauca acaucgcccg gaacaccaag gggaagugcg     2340

agaaggagau cuucaaccug aucugcaaga ucgagggcag cgaggacaag aaggggaacu     2400

acaagcacgg ccuggccuac gagcucgggg ugcugcuguu cggcgagccg aacgaggccu     2460

ccaagcccga guucgaccgc aagaucaaga aguucaacag caucuacucc uucgcgcaga     2520

uccagcagau cgccuucgcc gagcggaagg gcaacgccaa cacgugcgcg gucugcagcg     2580

ccgacaacgc ccaccgcaug cagcagauca agaucaccga gcccguggag gacaacaagg     2640

acaagaucau ccucuccgcc aaggcccagc ggcugcccgc gaucccgacc cgcaucgugg     2700

acggggccgu caagaagaug gccaccaucc uggccaagaa caucguggac gacaacuggc     2760

agaacaucaa gcagguccuc agcgccaagc accagcugca cauccccauc aucaccgagu     2820

ccaacgcguu cgaguucgag cccgcccugg ccgacgugaa gggcaagagc cucaaggacc     2880

ggcgcaagaa ggcccuggag cggaucuccc ccgagaacau cuucaaggac aagaacaacc     2940

gcaucaagga guucgccaag gggaucagcg cguacuccgg cgccaaccug accgacgggg     3000

acuucgacgg cgccaaggag gagcucgacc acaucauccc gcggagccac aagaaguacg     3060

gcacgcugaa cgacgaggcc aaccugaucu gcgugacccg cggggacaac aagaacaagg     3120

gcaaccgcau cuucugccuc cgggaccugg cggacaacua caagcugaag caguucgaga     3180

ccaccgacga ccucgagauc gagaagaaga ucgccgacac gaucugggac gccaacaaga     3240

aggacuucaa guucgggaac uaccgcuccu ucaucaaccu gacgccccag gagcagaagg     3300

ccuuccggca cgcccuguuc cucgcggacg agaaccccau caagcaggcc gugauccgcg     3360

ccaucaacaa ccggaaccgc accuucguca acggcaccca gcgguacuuc gccgaggugc     3420

uggccaacaa caucuaccug cgcgcgaaga aggagaaccu caacaccgac aagaucagcu     3480

ucgacuacuu cggcaucccc accaucggga acggccgggg gaucgccgag auccgccagc     3540

uguacgagaa gguggacucc gacauccagg ccuacgccaa gggcgacaag ccgcaggcca     3600

gcuacagcca ccugaucgac gcgaugcucg ccuucugcau cgccgccgac gagcaccgga     3660

acgacggguc caucggccug gagaucgaca agaacuacag ccuguacccc cucgacaaga     3720

acaccggcga ggucuucacc aaggacaucu ucucccagau caagaucacc gacaacgagu     3780

ucagcgacaa gaagcuggug cgcaagaagg cgaucgaggg guucaacacg caccggcaga     3840

ugacccgcga cggcaucuac gccgagaacu accugcccau ccucauccac aaggagcuga     3900

acgaggugcg gaagggguac accuggaaga acuccgagga gaucaagauc uucaagggca     3960

agaaguacga cauccagcag cugaacaacc ucgucuacug ccugaaguuc guggacaagc     4020

cgaucagcau cgacauccag aucuccaccc uggaggagcu ccgcaacauc cugacgacca     4080

acaacaucgc cgccaccgcc gaguacuacu acaucaaccu gaagacccag aagcuccacg     4140

aguacuacau cgagaacuac aacacggcgc ugggguacaa gaaguacagc aaggagaugg     4200

aguuccugcg gucccucgcc uaccgcagcg agcgggugaa gaucaagucc aucgacgacg     4260

ucaagcaggu ccuggacaag gacagcaacu ucaucaucgg caagaucacc cugcccuuca     4320

agaaggagug gcagcgccuc uaccgggagu ggcagaacac caccaucaag gacgacuacg     4380

aguuccugaa guccuucuuc aacgucaaga gcaucacgaa gcugcacaag aaggugcgca     4440

aggacuucuc ccuccccauc agcaccaacg agggcaaguu ccuggugaag cgcaagaccu     4500

gggacaacaa cuucaucuac cagauccuga acgacuccga cagccgggcc gacgggacca     4560

agcccuucau cccggccuuc gacaucucca agaacgagau cguggaggcc aucaucgaca     4620

gcuucacgag caagaacauc uucuggcucc ccaagaacau cgagcugcag aagguggaca     4680

acaagaacau cuucgcgauc gacaccucca agugguucga gguggagacc cccagcgacc     4740

ugcgcgacau cggcaucgcc accauccagu acaagaucga caacaacucc cggcccaagg     4800

ugcgcgucaa gcuggacuac gugaucgacg acgacagcaa gaucaacuac uucaugaacc     4860

acucccuccu caagagccgg uacccggaca aggugcugga gauccugaag caguccacga     4920

ucaucgaguu cgagagcucc ggguucaaca agaccaucaa ggagaugcug ggcaugaagc     4980

uggccgggau cuacaacgag accagcaaca acaagcgccc cgccgccacc aagaaggcgg     5040

gccaggccaa gaagaagaag uaaggacuag ucccuguucc cagagcccac uuuuuuuucu     5100

uuuuuugaaa uaaaauagcc ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     5160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                       5203


<210>  2396
<211>  3475
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2396
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gcggaacuac auccugggcc ucgacaucgg gaucaccagc gugggcuacg      180

ggaucaucga cuacgagacc cgcgacguga ucgacgccgg cguccggcug uucaaggagg      240

ccaacgugga gaacaacgag ggccgccggu ccaagcgcgg ggcccggcgc cugaagcggc      300

gccggcgcca ccggauccag cgcgugaaga agcuccuguu cgacuacaac cugcucacgg      360

accacagcga gcuguccggc aucaaccccu acgaggcccg ggucaagggg cugagccaga      420

agcucuccga ggaggaguuc agcgcggccc ugcugcaccu cgccaagcgc cggggcgugc      480

acaacgugaa cgaggucgag gaggacaccg ggaacgagcu guccaccaag gagcagauca      540

gccgcaacuc caaggcccug gaggagaagu acguggcgga gcuccagcug gagcggcuga      600

agaaggacgg cgaggugcgc ggcagcauca accgguucaa gaccuccgac uacgucaagg      660

aggccaagca gcuccugaag gugcagaagg ccuaccacca gcuggaccag agcuucaucg      720

acacguacau cgaccuccug gagacccgcc gcaccuacua cgaggggccc ggcgaggggu      780

cccccuucgg cuggaaggac aucaaggagu gguacgagau gcugaugggc cacugcaccu      840

acuucccgga ggagcuccgg agcgugaagu acgccuacaa cgccgaccug uacaacgcgc      900

ugaacgaccu caacaaccug gucaucacgc gcgacgagaa cgagaagcug gaguacuacg      960

agaaguucca gaucaucgag aacguguuca agcagaagaa gaagcccacc cucaagcaga     1020

ucgccaagga gauccuggug aacgaggagg acaucaaggg guaccggguc accuccaccg     1080

gcaagcccga guucacgaac cugaaggugu accacgacau caaggacauc accgcccgca     1140

aggagaucau cgagaacgcc gagcuccugg accagaucgc caagauccug accaucuacc     1200

agagcuccga ggacauccag gaggagcuca ccaaccugaa cagcgagcug acgcaggagg     1260

agaucgagca gaucuccaac cucaaggggu acaccggcac ccacaaccug agccugaagg     1320

cgaucaaccu cauccuggac gagcuguggc acaccaacga caaccagauc gccaucuuca     1380

accggcucaa gcuggugccc aagaaggucg accugagcca gcagaaggag aucccgacga     1440

cccucgugga cgacuucauc cugucccccg uggucaagcg cagcuucauc caguccauca     1500

aggugaucaa cgccaucauc aagaaguacg ggcugcccaa cgacaucauc aucgagcugg     1560

cccgggagaa gaacagcaag gacgcccaga agaugaucaa cgagaugcag aagcgcaacc     1620

ggcagaccaa cgagcgcauc gaggagauca uccggaccac gggcaaggag aacgcgaagu     1680

accucaucga gaagaucaag cugcacgaca ugcaggaggg caagugccug uacucccucg     1740

aggccauccc ccuggaggac cugcucaaca acccguucaa cuacgagguc gaccacauca     1800

ucccccgcag cguguccuuc gacaacagcu ucaacaacaa ggugcugguc aagcaggagg     1860

agaacuccaa gaaggggaac cggacccccu uccaguaccu gagcuccagc gacuccaaga     1920

ucagcuacga gaccuucaag aagcacaucc ucaaccuggc caagggcaag gggcgcaucu     1980

ccaagaccaa gaaggaguac cugcucgagg agcgggacau caaccgcuuc agcgugcaga     2040

aggacuucau caaccggaac cugguggaca cgcgcuacgc cacccggggc cugaugaacc     2100

uccugcgcuc cuacuuccgc gucaacaacc uggacgugaa ggucaagagc aucaacgggg     2160

gcuucaccuc cuuccuccgg cgcaagugga aguucaagaa ggagcggaac aagggcuaca     2220

agcaccacgc cgaggacgcg cugaucaucg ccaacgccga cuucaucuuc aaggagugga     2280

agaagcugga caaggccaag aaggugaugg agaaccagau guucgaggag aagcaggcgg     2340

agagcaugcc cgagaucgag accgagcagg aguacaagga gaucuucauc acgccgcacc     2400

agaucaagca caucaaggac uucaaggacu acaaguacag ccaccgcgug gacaagaagc     2460

ccaaccggga gcucaucaac gacacccugu acuccacccg caaggacgac aaggggaaca     2520

cccugaucgu gaacaaccuc aacggccugu acgacaagga caacgacaag cugaagaagc     2580

ucaucaacaa gagccccgag aagcugcuga uguaccacca cgacccccag acguaccaga     2640

agcucaagcu gaucauggag caguacgggg acgagaagaa cccgcuguac aaguacuacg     2700

aggagaccgg caacuaccuc accaaguacu ccaagaagga caacgggccc gucaucaaga     2760

agaucaagua cuacggcaac aagcugaacg cccaccugga caucaccgac gacuacccca     2820

acagccggaa caagguggug aagcucuccc ugaagcccua ccgcuucgac gucuaccugg     2880

acaacggcgu guacaaguuc gugaccguca agaaccucga cgugaucaag aaggagaacu     2940

acuacgaggu gaacagcaag ugcuacgagg aggccaagaa gcugaagaag aucuccaacc     3000

aggccgaguu caucgccagc uucuacaaca acgaccugau caagaucaac ggggagcucu     3060

accgggucau cggcgugaac aacgaccugc ugaaccgcau cgaggugaac augaucgaca     3120

ucacguaccg ggaguaccuc gagaacauga acgacaagcg cccgccccgg aucaucaaga     3180

ccaucgcguc caagacccag agcaucaaga aguacuccac cgacauccug gggaaccugu     3240

acgaggucaa gagcaagaag cacccccaga ucaucaagaa gggcaagcgc cccgccgcca     3300

cgaagaaggc cggccaggcc aagaagaaga aguaaggacu agucccuguu cccagagccc     3360

acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa aaaaaaaaaa     3420

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu          3475


<210>  2397
<211>  4543
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2397
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgcu guucaacaag ugcaucauca ucagcaucaa ccucgacuuc uccaacaagg      180

agaagugcau gaccaagccc uacagcaucg gccuggacau cgggaccaac uccgugggcu      240

gggccgugau cacggacaac uacaaggucc ccagcaagaa gaugaaggug cuggggaaca      300

ccuccaagaa guacaucaag aagaaccucc ugggcgugcu gcuguucgac agcggcauca      360

ccgccgaggg gcggcgccug aagcggaccg cccgccggcg cuacacgcgg cgccggaacc      420

gcauccugua ccuccaggag aucuucucca ccgagauggc cacccuggac gacgcguucu      480

uccagcggcu ggacgacagc uuccucgucc ccgacgacaa gcgcgacucc aaguacccga      540

ucuucggcaa ccugguggag gagaaggugu accacgacga guuccccacc aucuaccacc      600

uccggaagua ccucgccgac agcacgaaga aggccgaccu gcgccugguc uaccucgccc      660

uggcgcacau gaucaaguac cgggggcacu uccugaucga gggcgaguuc aacuccaaga      720

acaacgacau ccagaagaac uuccaggacu uccucgacac cuacaacgcc aucuucgaga      780

gcgaccuguc ccuggagaac agcaagcagc ucgaggagau cgugaaggac aagaucucca      840

agcuggagaa gaaggaccgc auccugaagc uguuccccgg ggagaagaac agcggcaucu      900

ucuccgaguu ccugaagcug aucgugggca accaggccga cuuccggaag ugcuucaacc      960

ucgacgagaa ggccagccug cacuucucca aggagagcua cgacgaggac cuggagaccc     1020

uccuggggua caucggcgac gacuacagcg acgucuuccu gaaggccaag aagcucuacg     1080

acgcgauccu gcuguccggg uuccucaccg ugacggacaa cgagaccgag gcgccccuga     1140

gcuccgccau gaucaagcgc uacaacgagc acaaggagga ccuggcccuc cugaaggagu     1200

acauccggaa caucagccug aagaccuaca acgagguguu caaggacgac accaagaacg     1260

gcuacgccgg cuacaucgac gggaagacga accaggagga cuucuacguc uaccucaaga     1320

accugcuggc cgaguucgag ggcgccgacu acuuccucga gaagaucgac cgcgaggacu     1380

uccugcggaa gcagcgcacc uucgacaacg gguccauccc guaccagauc caccuccagg     1440

agaugcgcgc cauccuggac aagcaggcca aguucuaccc cuuccucgcc aagaacaagg     1500

agcggaucga gaagauccug accuuccgca uccccuacua cgugggcccc cuggcgcggg     1560

ggaacagcga cuucgccugg uccauccgca agcggaacga gaagaucacc ccguggaacu     1620

ucgaggacgu gaucgacaag gagagcuccg ccgaggccuu caucaaccgc augacgagcu     1680

ucgaccucua ccugcccgag gagaaggucc ugcccaagca cucccuccug uacgagaccu     1740

ucaacgugua caacgagcug accaaggugc gguucaucgc cgagagcaug cgcgacuacc     1800

aguuccucga cuccaagcag aagaaggaca ucguccggcu guacuucaag gacaagcgca     1860

aggugaccga caaggacauc aucgaguacc ugcacgcgau cuacggcuac gacggcaucg     1920

agcucaaggg gaucgagaag caguucaaca gcucccugag cacguaccac gaccugcuca     1980

acaucaucaa cgacaaggag uuccuggacg acuccagcaa cgaggccauc aucgaggaga     2040

ucauccacac ccugaccauc uucgaggacc gggagaugau caagcagcgc cucuccaagu     2100

ucgagaacau cuucgacaag agcgugcuga agaagcugag ccggcgccac uacaccggcu     2160

gggggaagcu cuccgccaag cugaucaacg gcauccggga cgagaagucc gggaacacga     2220

uccuggacua ccucaucgac gacggcauca gcaaccgcaa cuucaugcag cugauccacg     2280

acgacgcccu gagcuucaag aagaagaucc agaaggcgca gaucaucggc gacgaggaca     2340

aggggaacau caaggagguc gugaaguccc uccccggcag cccggccauc aagaagggga     2400

uccugcaguc caucaagauc gucgacgagc uggugaaggu gaugggcggg cggaagcccg     2460

agagcaucgu cguggagaug gcccgcgaga accaguacac caaccagggc aaguccaaca     2520

gccagcagcg gcucaagcgc cuggagaagu cccugaagga gcucggcagc aagauccuga     2580

aggagaacau ccccgccaag cuguccaaga ucgacaacaa cgcccuccag aacgaccgcc     2640

uguaccugua cuaccuccag aacgggaagg acauguacac cggcgacgac cuggacaucg     2700

accggcugag caacuacgac aucgaccaca ucauccccca ggcguuccuc aaggacaacu     2760

ccaucgacaa caaggugcug gucagcuccg ccagcaaccg cgggaagucc gacgacuucc     2820

cgagccugga gguggugaag aagcggaaga ccuucuggua ccagcuccuc aagagcaagc     2880

ugaucuccca gcgcaaguuc gacaaccuga cgaaggccga gcggggcggc cugcugcccg     2940

aggacaaggc cggguucauc cagcgccagc ucgucgagac ccggcagauc accaagcacg     3000

uggcccgccu gcuggacgag aaguucaaca acaagaagga cgagaacaac cgggcggugc     3060

gcaccgucaa gaucaucacg cucaagagca cccugguguc ccaguuccgg aaggacuucg     3120

agcuguacaa ggugcgcgag aucaacgacu uccaccacgc ccacgacgcc uaccucaacg     3180

ccgucaucgc cagcgcgcug cugaagaagu accccaagcu cgagcccgag uucguguacg     3240

gcgacuaccc gaaguacaac uccuuccggg agcgcaagag cgccaccgag aagguguacu     3300

ucuacuccaa caucaugaac aucuucaaga aguccaucag ccuggccgac gggcggguca     3360

ucgagcgccc ccugaucgag gugaacgagg agaccggcga gagcgugugg aacaaggagu     3420

ccgaccucgc caccguccgg cgcgugcuga gcuaccccca ggugaacguc gugaagaagg     3480

uggaggagca gaaccacggg cuggaccggg gcaagcccaa gggccucuuc aacgcgaacc     3540

uguccagcaa gccgaagccc aacuccaacg agaaccuggu cggggccaag gaguaccucg     3600

accccaagaa guacggcggg uacgccggca ucagcaacuc cuucgccgug cuggugaagg     3660

ggacgaucga gaagggcgcc aagaagaaga ucaccaacgu ccuggaguuc cagggcauca     3720

gcauccucga ccgcaucaac uaccggaagg acaagcugaa cuuccugcuc gagaaggggu     3780

acaaggacau cgagcugauc aucgagcugc cgaaguacag ccucuucgag cuguccgacg     3840

gcagccgccg caugcuggcg uccauccuca gcaccaacaa caagcgcggg gagauccaca     3900

agggcaacca gaucuuccug ucccagaagu ucgugaagcu gcucuaccac gccaagcgga     3960

ucagcaacac caucaacgag aaccaccgga aguacgugga gaaccacaag aaggaguucg     4020

aggagcuguu cuacuacauc cuggaguuca acgagaacua cgucggggcc aagaagaacg     4080

gcaagcuccu gaacuccgcc uuccagagcu ggcagaacca cuccaucgac gagcugugca     4140

gcuccuucau cggccccacg gggagcgagc gcaagggccu cuucgagcug accucccggg     4200

ggagcgccgc ggacuucgag uuccugggcg ugaagauccc ccgcuaccgg gacuacaccc     4260

ccuccagccu ccugaaggac gccacccuga uccaccaguc cgugacgggg cuguacgaga     4320

cccgcaucga ccucgccaag cugggcgagg gcaagcggcc ggccgccacc aagaaggcgg     4380

ggcaggccaa gaagaagaag uaaggacuag ucccuguucc cagagcccac uuuuuuuucu     4440

uuuuuugaaa uaaaauagcc ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                       4543


<210>  2398
<211>  3619
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2398
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgug guacgccagc cugauguccg cccaccaccu ccgggugggc aucgacgugg      180

ggacccacag cgucggccug gccacccugc gcguggacga ccacgggacg cccaucgagc      240

uccuguccgc ccugagccac auccacgacu ccggcguggg caaggagggg aagaaggacc      300

acgacacccg gaagaagcuc agcggcaucg cgcgccgggc ccgccggcug cugcaccacc      360

gccggaccca gcuccagcag cuggacgagg uccugcgcga ccucggguuc cccaucccca      420

ccccgggcga guuccuggac cugaacgagc agacggaccc cuaccgggug uggcgcgugc      480

gggcccgccu cgucgaggag aagcugcccg aggagcugcg ggggcccgcc aucuccaugg      540

cggugcgcca caucgcccgg caccgcggcu ggcggaaccc guacagcaag guggaguccc      600

uccugagccc cgccgaggag ucccccuuca ugaaggcccu gcgcgagcgg auccucgcca      660

ccaccggcga gguccuggac gacgggauca cccccggcca ggcgauggcc cagguggccc      720

ugacgcacaa caucagcaug cgcgggccgg agggcauccu cggcaagcug caccaguccg      780

acaacgccaa cgagauccgc aagaucugcg cccggcaggg ggugagcccc gacgucugca      840

agcagcugcu ccgcgcggug uucaaggccg acagcccccg gggcuccgcc gugucccgcg      900

ucgcccccga cccgcugccc gggcagggca gcuuccggcg cgcccccaag ugcgaccccg      960

aguuccagcg guuccgcauc aucuccaucg uggcgaaccu gcggaucagc gagaccaagg     1020

gggagaaccg cccgcucacc gccgacgagc ggcgccacgu ggucaccuuc cugacggagg     1080

acagccaggc cgaccugacc uggguggacg uggccgagaa gcucggcguc caccggcgcg     1140

accugcgggg caccgccgug cacaccgacg acggggagcg cuccgcggcc cggccgccca     1200

ucgacgccac ggaccgcauc augcggcaga ccaagaucag cucccugaag accugguggg     1260

aggaggccga cagcgagcag cgcggcgcga ugauccgcua ccucuacgag gaccccaccg     1320

acuccgagug cgccgagauc aucgccgagc ugcccgagga ggaccaggcc aagcuggaca     1380

gccuccaccu ccccgccggg cgggcggccu acucccgcga gagccugacg gcccuguccg     1440

accacaugcu ggccaccacc gacgaccugc acgaggcccg gaagcgccuc uucggcgucg     1500

acgacagcug ggcgccgccc gccgaggcca ucaacgcccc cguggggaac cccuccgugg     1560

accggacccu gaagaucguc ggccgcuacc ugagcgccgu ggaguccaug uggggcacgc     1620

ccgaggugau ccacgucgag cacgugcggg acggguucac cagcgagcgc auggcggacg     1680

agcgggacaa ggccaaccgc cggcgcuaca acgacaacca ggaggccaug aagaagaucc     1740

agcgggacua cggcaaggag ggguacaucu cccgcggcga caucgugcgg cucgacgccc     1800

uggagcugca ggggugcgcg ugccuguacu gcggcaccac caucggcuac cacacgugcc     1860

agcucgacca caucgucccg caggccgggc ccggcagcaa caaccgccgg gggaaccugg     1920

uggccgugug cgagcgcugc aaccggucca agagcaacac ccccuucgcc gucugggccc     1980

agaagugcgg caucccccac gugggcguga aggaggcgau cgggcgcguc cggggcuggc     2040

gcaagcagac cccgaacacc uccagcgagg accugacgcg ccucaagaag gaggugaucg     2100

cccggcugcg ccggacccag gaggaccccg agaucgacga gcgcagcaug gaguccgugg     2160

ccuggauggc caacgagcug caccaccgga ucgccgcggc cuaccccgag accaccguca     2220

ugguguaccg cgggagcauc acggccgccg cccggaaggc ggccggcauc gacucccgca     2280

ucaaccucau cggggagaag ggccggaagg accgcaucga ccggcgccac cacgccgugg     2340

acgccagcgu cguggcgcug auggaggccu ccguggccaa gacccuggcc gagcggagcu     2400

cccuccgcgg cgagcagcgg cugaccggga aggagcagac cuggaagcag uacaccggca     2460

gcacggucgg ggcccgcgag cacuucgaga uguggcgggg ccacaugcug caccucaccg     2520

agcuguucaa cgagcgccug gcggaggaca agguguacgu gacccagaac auccggcucc     2580

gccuguccga cgggaacgcc cacaccguca accccagcaa gcuggugucc caccgccucg     2640

gcgacggccu gacggugcag cagaucgacc gggccugcac cccggcccug uggugcgccc     2700

ucacccgcga gaaggacuuc gacgagaaga acgggcugcc cgcgcgggag gaccgcgcca     2760

uccgggucca cggccacgag aucaagagcu ccgacuacau ccagguguuc agcaagcgca     2820

agaagaccga cuccgaccgg gacgagacgc ccuucggggc caucgccgug cgcggcgggu     2880

ucgucgagau cggccccagc auccaccacg cccggaucua ccgcguggag ggcaagaagc     2940

cgguguacgc gaugcugcgg gucuucaccc acgaccuccu gucccagcgc cacggggacc     3000

uguucagcgc cgugaucccg ccccagagca ucuccaugcg gugcgccgag cccaagcucc     3060

gcaaggccau caccaccggc aacgccacgu accuggggug gguggucgug ggcgacgagc     3120

uggagaucaa cguggacagc uucaccaagu acgcgaucgg gcgguuccuc gaggacuucc     3180

ccaacaccac ccgcuggcgg aucugcggcu acgacacgaa cuccaagcug acccugaagc     3240

ccaucguccu cgccgccgag ggccuggaga accccagcuc cgccgugaac gagaucgugg     3300

agcugaaggg guggcgcguc gcgaucaacg ugcucaccaa ggugcacccc accgucgugc     3360

ggcgcgacgc ccugggccgg ccgcgcuaca gcucccgcag caaccugccc acguccugga     3420

ccaucgagaa gcggcccgcc gccaccaaga aggccgggca ggcgaagaag aagaaguaag     3480

gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc     3540

uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaagaauu                                                  3619


<210>  2399
<211>  4480
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2399
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgac caagcccuac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccgugacgac cgacaacuac aaggucccca gcaagaagau gaaggugcuc gggaacaccu      240

ccaagaagua caucaagaag aaccugcugg gcgugcuccu guucgacagc ggcaucaccg      300

ccgaggggcg gcgccugaag cggacggccc gccggcgcua cacccggcgc cggaaccgca      360

uccucuaccu gcaggagauc uucuccaccg agauggccac ccuggacgac gcguucuucc      420

agcggcucga cgacagcuuc cugguccccg acgacaagcg cgacuccaag uacccgaucu      480

ucggcaaccu gguggaggag aaggccuacc acgacgaguu ccccacgauc uaccaccucc      540

ggaaguaccu ggccgacagc accaagaagg ccgaccugcg ccucguguac cuggcgcugg      600

cccacaugau caaguaccgg gggcacuucc ucaucgaggg cgaguucaac uccaagaaca      660

acgacaucca gaagaacuuc caggacuucc uggacaccua caacgccauc uucgagagcg      720

accugucccu cgagaacagc aagcagcugg aggagaucgu caaggacaag aucuccaagc      780

uggagaagaa ggaccgcauc cucaagcugu uccccgggga gaagaacagc ggcaucuucu      840

ccgaguuccu gaagcucauc gugggcaacc aggccgacuu ccggaagugc uucaaccugg      900

acgagaaggc cagccugcac uucuccaagg agagcuacga cgaggaccuc gagacccugc      960

ugggguacau cggcgacgac uacuccgacg uguuccucaa ggcgaagaag cuguacgacg     1020

ccauccugcu cagcggguuc cugacgguca ccgacaacga gaccgaggcg ccccugagcu     1080

ccgccaugau caagcgcuac aacgagcaca aggaggaccu cgcccugcug aaggaguaca     1140

uccggaacau cagccucaag accuacaacg agguguucaa ggacgacacg aagaacggcu     1200

acgccggcua caucgacggg aagaccaacc aggaggacuu cuacguguac cugaagaagc     1260

ugcucgccga guucgagggc gccgacuacu uccuggagaa gaucgaccgc gaggacuucc     1320

ugcggaagca gcgcaccuuc gacaacgggu ccaucccgua ccagauccac cuccaggaga     1380

ugcgcgccau ccuggacaag caggccaagu ucuaccccuu ccuggcgaag aacaaggagc     1440

ggaucgagaa gauccugacc uuccgcaucc ccuacuacgu cggcccccuc gcccggggga     1500

acagcgacuu cgccuggucc auccgcaagc ggaacgagaa gaucacgccg uggaacuucg     1560

aggacgugau cgacaaggag agcuccgccg aggccuucau caaccgcaug accagcuucg     1620

accuguaccu gcccgaggag aaggugcucc ccaagcacuc ccugcuguac gagaccuuca     1680

acgucuacaa cgagcucacc aaggugcggu ucaucgcgga gagcaugcgc gacuaccagu     1740

uccuggacuc caagcagaag aaggacaucg ugcggcugua cuucaaggac aagcgcaagg     1800

ucacggacaa ggacaucauc gaguaccucc acgccaucua cggcuacgac ggcaucgagc     1860

ugaaggggau cgagaagcag uucaacagcu cccugagcac cuaccacgac cuccugaaca     1920

ucaucaacga caaggaguuc cuggacgacu ccagcaacga ggccaucauc gaggagauca     1980

uccacacccu caccaucuuc gaggaccggg agaugaucaa gcagcgccug uccaaguucg     2040

agaacaucuu cgacaagagc gugcugaaga agcucucccg gcgccacuac acgggcuggg     2100

ggaagcugag cgccaagcug aucaacggca uccgggacga gaaguccggg aacaccaucc     2160

ucgacuaccu gaucgacgac ggcaucagca accgcaacuu caugcagcug auccacgacg     2220

acgcgcucag cuucaagaag aagauccaga aggcccagau caucggcgac gaggacaagg     2280

ggaacaucaa ggaggugguc aagucccugc ccggcagccc ggccaucaag aaggggaucc     2340

ugcaguccau caagaucgug gacgagcucg ucaaggugau gggcgggcgg aagcccgaga     2400

gcaucguggu cgagauggcc cgcgagaacc aguacaccaa ccagggcaag uccaacagcc     2460

agcagcggcu gaagcgccug gagaaguccc ucaaggagcu gggcagcaag auccugaagg     2520

agaacauccc cgccaagcuc uccaagaucg acaacaacgc gcugcagaac gaccgccugu     2580

accucuacua ccugcagaac gggaaggaca uguacaccgg cgacgaccug gacaucgacc     2640

ggcucagcaa cuacgacauc gaccacauca ucccccaggc cuuccugaag gacaacucca     2700

ucgacaacaa ggugcuggug agcuccgcca gcaaccgcgg gaaguccgac gacgucccga     2760

gccucgaggu ggugaagaag cggaagacgu ucugguacca gcugcucaag agcaagcuga     2820

ucucccagcg caaguucgac aaccugacca aggccgagcg gggcggccug agccccgagg     2880

acaaggccgg guucauccag cgccagcucg ucgagacccg gcagaucacc aagcacgugg     2940

cgcgccugcu ggacgagaag uucaacaaca agaaggacga gaacaaccgg gccgugcgca     3000

cggucaagau caucacccuc aaguccaccc uggugagcca guuccggaag gacuucgagc     3060

uguacaaggu gcgcgagauc aacgacuucc accacgccca cgacgccuac cucaacgccg     3120

ucguggcguc cgcccugcug aagaaguacc ccaagcucga gcccgaguuc guguacggcg     3180

acuacccgaa guacaacagc uuccgggagc gcaaguccgc caccgagaag gucuacuucu     3240

acagcaacau caugaacauc uucaagaagu ccaucagccu ggccgacggg cgggugaucg     3300

agcgcccccu gaucgaggug aacgaggaga ccggcgaguc cgucuggaac aaggagagcg     3360

accucgcgac ggugcggcgc gugcuguccu acccccaggu caacguggug aagaaggucg     3420

aggagcagaa ccacgggcug gaccggggca agcccaaggg ccucuucaac gccaaccuga     3480

gcuccaagcc gaagcccaac agcaacgaga accugguggg ggccaaggag uaccucgacc     3540

ccaagaagua cggcggguac gccggcaucu ccaacagcuu caccgugcug gucaagggga     3600

ccaucgagaa gggcgccaag aagaagauca ccaacgugcu ggaguuccag ggcaucucca     3660

uccucgaccg caucaacuac cggaaggaca agcugaacuu ccugcucgag aagggguaca     3720

aggacaucga gcugaucauc gagcugccga aguacagccu cuucgagcug agcgacggcu     3780

cccgccgcau gcuggcgagc auccucucca cgaacaacaa gcgcggggag auccacaagg     3840

gcaaccagau cuuccugagc cagaaguucg ugaagcugcu cuaccacgcc aagcggaucu     3900

ccaacaccau caacgagaac caccggaagu acgucgagaa ccacaagaag gaguucgagg     3960

agcuguucua cuacauccug gaguucaacg agaacuacgu gggggccaag aagaacggca     4020

agcuccugaa cagcgccuuc caguccuggc agaaccacag caucgacgag cugugcucca     4080

gcuucaucgg ccccaccggg uccgagcgca agggccucuu cgagcugacc agccgggggu     4140

ccgccgcgga cuucgaguuc cugggcguga agaucccccg cuaccgggac uacacgccca     4200

gcucccuccu gaaggacgcc acccugaucc accagagcgu caccgggcuc uacgagaccc     4260

gcaucgaccu ggccaagcug ggcgagggca agcggccggc cgccacgaag aaggcggggc     4320

aggccaagaa gaagaaguaa ggacuagucc cuguucccag agcccacuuu uuuuucuuuu     4380

uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                           4480


<210>  2400
<211>  3562
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2400
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc cgccuucaag cccaacagca ucaacuacau ccugggccuc gacaucggga      180

ucgccuccgu gggcugggcc augguggaga ucgacgagga ggagaacccc auccggcuga      240

ucgaccuggg gguccgcgug uucgagcggg cggaggugcc caagaccggc gacagccucg      300

ccauggcccg ccggcuggcc cgcuccgucc ggcgccugac ccggcgccgg gcgcaccgcc      360

uccugcggac gcgccggcug cucaagcgcg agggcgugcu gcaggccgcc aacuucgacg      420

agaacgggcu gaucaagagc cucccgaaca cccccuggca gcugcgggcc gccgcgcugg      480

accgcaagcu cacgccccug gagugguccg ccgugcugcu ccaccugauc aagcaccggg      540

gcuaccugag ccagcgcaag aacgaggggg agaccgccga caaggagcuc ggcgcccugc      600

ugaagggggu cgccggcaac gcgcacgccc uccagaccgg cgacuuccgg acccccgccg      660

agcuggcccu gaacaaguuc gagaaggagu ccgggcacau ccgcaaccag cgcagcgacu      720

acucccacac cuucagccgg aaggaccucc aggccgagcu gauccuccug uucgagaagc      780

agaaggaguu cggcaacccg cacguguccg ggggccugaa ggagggcauc gagacccugc      840

ucaugacgca gcgccccgcg cugagcgggg acgccgugca gaagaugcug ggccacugca      900

ccuucgagcc cgccgagccc aaggccgcca agaacaccua caccgcggag cgguucaucu      960

ggcucacgaa gcugaacaac cugcgcaucc ucgagcaggg guccgagcgg ccgcugaccg     1020

acaccgagcg cgccacccug auggacgagc ccuaccgcaa gagcaagcuc acguacgccc     1080

aggcccggaa gcugcugggc cucgaggaca ccgcguucuu caaggggcug cgguacggca     1140

aggacaacgc cgaggccucc acccugaugg agaugaaggc cuaccacgcc aucagccgcg     1200

cgcucgagaa ggagggccug aaggacaaga aguccccgcu gaaccugagc cccgagcucc     1260

aggacgagau cgggaccgcc uucucccugu ucaagacgga cgaggacauc accggccggc     1320

ugaaggaccg cauccagccg gagauccucg aggcccugcu gaagcacauc agcuucgaca     1380

aguucgucca gaucagccuc aaggcccugc ggcgcaucgu gccccugaug gagcagggga     1440

agcgguacga cgaggccugc gcggagaucu acggcgacca cuacgggaag aagaacaccg     1500

aggagaagau cuaccucccg cccauccccg ccgacgagau ccgcaacccc gugguccugc     1560

gggcccuguc ccaggcccgc aaggugauca acggcguggu ccggcgcuac ggcagccccg     1620

cccgcaucca caucgagacc gcgcgggagg uggggaaguc cuucaaggac cgcaaggaga     1680

ucgagaagcg gcaggaggag aaccgcaagg accgggagaa ggccgccgcc aaguuccgcg     1740

aguacuuccc caacuucgug ggcgagccca agagcaagga cauccucaag cugcggcugu     1800

acgagcagca gcacgggaag ugccucuacu ccggcaagga gaucaaccug gggcgccuga     1860

acgagaaggg cuacgucgag aucgaccacg cgcuccccuu cagccggacg ugggacgacu     1920

ccuucaacaa caaggugcug gugcugggca gcgagaacca gaacaagggg aaccagaccc     1980

ccuacgagua cuucaacggc aaggacaacu cccgcgagug gcaggaguuc aaggcccggg     2040

ucgagaccag ccgcuucccc cgguccaaga agcagcgcau ccuccugcag aaguucgacg     2100

aggacggguu caaggagcgg aaccugaacg acacccgcua cgugaaccgg uuccucugcc     2160

aguucgucgc cgaccgcaug cggcugacgg gcaagggcaa gaagcgcgug uucgccagca     2220

acgggcagau caccaaccug cuccggggcu ucugggggcu gcgcaaggug cgcgccgaga     2280

acgaccggca ccacgcgcug gacgccgucg ugguggccug cuccaccguc gccaugcagc     2340

agaagaucac ccgcuucgug cgguacaagg agaugaacgc cuucgacggc aagacgaucg     2400

acaaggagac cggggaggug cuccaccaga agacccacuu cccgcagccc ugggaguucu     2460

ucgcgcagga ggucaugauc cgcguguucg gcaagcccga cggcaagccg gaguucgagg     2520

aggccgacac ccuggagaag cugcggacgc uccuggccga gaagcugagc ucccgccccg     2580

aggccgugca cgaguacguc acgccccucu ucgugagccg ggcccccaac cgcaagaugu     2640

ccgggcaggg ccacauggag accgucaaga gcgcgaagcg gcuggacgag gggguguccg     2700

ugcugcgcgu gccgcucacc cagcugaagc ugaaggaccu cgagaagaug gucaaccggg     2760

agcgcgagcc caagcuguac gaggcccuga aggcccggcu cgaggcccac aaggacgacc     2820

ccgcgaaggc cuucgccgag cccuucuaca aguacgacaa ggccggcaac cgcacccagc     2880

aggucaaggc cgugcgggug gagcaggucc agaagaccgg ggugugggug cgcaaccaca     2940

acggcaucgc ggacaacgcc accauggugc ggguggacgu cuucgagaag ggcgacaagu     3000

acuaccuggu gccgaucuac agcuggcagg ucgccaaggg gauccugccc gaccgcgccg     3060

uggugcaggg caaggacgag gaggacuggc agcucaucga cgacagcuuc aacuucaagu     3120

ucucccugca ccccaacgac cugguggagg ucaucaccaa gaaggcccgg auguucgggu     3180

acuucgcgag cugccaccgc ggcaccggga acaucaacau ccgcauccac gaccucgacc     3240

acaagaucgg caagaacggc auccuggagg ggaucggcgu gaagacggcc cuguccuucc     3300

agaaguacca gaucgacgag cucgggaagg agauccggcc cugccgccug aagaagcggc     3360

cgcccgugcg caagcggccc gccgccacca agaaggccgg ccaggcgaag aagaagaagu     3420

aaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu     3480

gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaagaa uu                                              3562


<210>  2401
<211>  4318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2401
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gaagcccuac accaucggcc uggacaucgg gaccaacagc gugggcuggg      180

ccgugcucac ggaccaguac gaccugguca agcggaagau gaagaucgcc ggggacuccg      240

agaagaagca gaucaagaag aacuucuggg gcgugcgccu guucgacgag ggccagaccg      300

ccgccgaccg gcgcauggcg cggaccgccc gccggcgcau cgagcggcgc cggaaccgca      360

ucagcuaccu ccaggggauc uucgccgagg agauguccaa gaccgacgcc aacuucuucu      420

gccggcugag cgacuccuuc uacguggaca acgagaagcg caacagccgg caccccuucu      480

ucgcgacgau cgaggaggag gucgaguacc acaagaacua ccccaccauc uaccaccucc      540

gcgaggagcu ggugaacucc agcgagaagg ccgaccugcg gcugguguac cucgcccugg      600

cccacaucau caaguaccgc ggcaacuucc ugaucgaggg ggcccucgac acccagaaca      660

ccuccgucga cggcaucuac aagcaguuca uccagacgua caaccaggug uucgcgagcg      720

ggaucgagga cggcucccug aagaagcugg aggacaacaa ggacguggcc aagauccucg      780

ucgagaaggu gacccggaag gagaagcugg agcgcauccu gaagcucuac ccgggcgaga      840

aguccgccgg gauguucgcc caguucauca gccugaucgu gggcagcaag gggaacuucc      900

agaagcccuu cgaccugauc gagaaguccg acaucgagug cgccaaggac agcuacgagg      960

aggaccucga gucccugcug gcgcucaucg gcgacgagua cgccgagcug uucgucgccg     1020

ccaagaacgc cuacagcgcg guggugcugu ccagcaucau caccgucgcc gagaccgaga     1080

cgaacgccaa gcucagcgcc uccaugaucg agcgguucga cacccacgag gaggaccugg     1140

gcgagcugaa ggccuucauc aagcuccacc uccccaagca cuacgaggag aucuucagca     1200

acaccgagaa gcacggguac gcgggcuaca ucgacgggaa gaccaagcag gccgacuucu     1260

acaaguacau gaagaugacg cuggagaaca ucgagggcgc cgacuacuuc aucgccaaga     1320

ucgagaagga gaacuuccug cgcaagcagc gcaccuucga caacggggcg aucccccacc     1380

agcugcaccu ggaggagcuc gaggccaucc ugcaccagca ggccaaguac uacccguucc     1440

ugaaggagaa cuacgacaag aucaaguccc ucgugaccuu ccggaucccc uacuucgugg     1500

gcccccuggc caacggccag agcgaguucg ccuggcugac ccgcaaggcg gacggggaga     1560

uccggcccug gaacaucgag gagaaggucg acuucggcaa guccgccgug gacuucaucg     1620

agaagaugac gaacaaggac accuaccucc cgaaggagaa cgugcugccc aagcacagcc     1680

ugugcuacca gaaguaccuc gucuacaacg agcugaccaa ggugcgcuac aucaacgacc     1740

aggggaagac cuccuacuuc agcggccagg agaaggagca gaucuucaac gaccuguuca     1800

agcagaagcg gaaggucaag aagaaggacc ucgagcuguu ccugcgcaac augucccacg     1860

uggagagccc cacgaucgag gggcuggagg acuccuucaa cagcuccuac agcaccuacc     1920

acgaccuccu gaaggugggc aucaagcagg agauccugga caaccccguc aacaccgaga     1980

ugcucgagaa caucgugaag auccugaccg uguucgagga caagcggaug aucaaggagc     2040

agcugcagca guucuccgac guccucgacg gcguggugcu gaagaagcug gagcgccggc     2100

acuacacggg guggggccgc cucagcgcca agcugcugau ggggauccgg gacaagcagu     2160

cccaccucac cauccuggac uaccugauga acgacgacgg ccucaaccgc aaccugaugc     2220

agcugaucaa cgacagcaac cucuccuuca agagcaucau cgagaaggag caggucacca     2280

ccgccgacaa ggacauccag agcaucgugg ccgaccuggc gggguccccg gccaucaaga     2340

agggcauccu gcagagccuc aagaucgugg acgagcuggu cuccgugaug ggcuacccgc     2400

cccagacgau cguggucgag auggcccggg agaaccagac caccgggaag ggcaagaaca     2460

acagccgccc ccgguacaag ucccuggaga aggccaucaa ggaguucggg agccagaucc     2520

ucaaggagca ccccaccgac aaccaggagc ugcgcaacaa ccggcuguac cucuacuacc     2580

ugcagaacgg caaggacaug uacacgggcc aggaccugga cauccacaac cucuccaacu     2640

acgacaucga ccacaucgug ccccagagcu ucaucaccga caacuccauc gacaaccugg     2700

ugcugaccag cuccgccggg aaccgcgaga agggcgacga cgucccgccc cucgagaucg     2760

ugcggaagcg caagguguuc ugggagaagc uguaccaggg gaaccugaug agcaagcgca     2820

aguucgacua ccucaccaag gcggagcggg gcgggcugac cgaggccgac aaggcccgcu     2880

ucauccaccg gcagcugguc gagacgcgcc agaucaccaa gaacguggcc aacauccucc     2940

accagcgguu caacuacgag aaggacgacc acggcaacac caugaagcag guccgcaucg     3000

ugacccugaa guccgcgcug gugagccagu uccggaagca guuccagcuc uacaaggugc     3060

gcgacgucaa cgacuaccac cacgcccacg acgccuaccu gaacggcgug guggccaaca     3120

cgcugcucaa ggucuacccc cagcuggagc ccgaguucgu guacggggac uaccaccagu     3180

ucgacugguu caaggccaac aaggcgaccg ccaagaagca guucuacacc aacaucaugc     3240

uguucuucgc ccagaaggac cggaucaucg acgagaacgg cgagauccuc ugggacaaga     3300

aguaccugga caccgugaag aaggucaugu ccuaccgcca gaugaacauc gugaagaaga     3360

cggagaucca gaagggggag uucagcaagg ccaccaucaa gcccaagggc aacuccagca     3420

agcugauccc ccggaagacc aacugggacc cgaugaagua cgggggccuc gacagcccca     3480

acauggccua cgcggugguc aucgaguacg ccaagggcaa gaacaagcug guguucgaga     3540

agaagaucau ccgcgugacc aucauggagc ggaaggccuu cgagaaggac gagaaggccu     3600

uccuggagga gcagggguac cgccagccca agguccucgc caagcugccg aaguacacgc     3660

uguacgagug cgaggagggc cggcgccgga ugcucgcguc cgccaacgag gcccagaagg     3720

ggaaccagca gguccugccc aaccaccugg ugacccuccu gcaccacgcc gcgaacugcg     3780

aggugagcga cggcaagucc cuggacuaca ucgagagcaa ccgcgagaug uucgccgagc     3840

uccuggccca cguguccgag uucgccaagc gguacacccu ggccgaggcg aaccucaaca     3900

agaucaacca gcuguucgag cagaacaagg agggggacau caaggccauc gcccagagcu     3960

ucguggaccu gauggccuuc aacgccaugg gcgcgcccgc cuccuucaag uucuucgaga     4020

ccacgaucga gcgcaagcgg uacaacaacc ucaaggagcu gcugaacagc accaucaucu     4080

accaguccau caccggccuc uacgagagcc gcaagcgccu ggacgacaag cggcccgccg     4140

ccaccaagaa ggccgggcag gcgaagaaga agaaguaagg acuagucccu guucccagag     4200

cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu       4318


<210>  2402
<211>  4351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2402
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gaagcccuac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccguggucac cgacgacuac aaggugcccg ccaagaagau gaaggugcuc gggaacacgg      240

acaagagcca caucgagaag aaccugcugg gcgcccuccu guucgacucc ggcaacaccg      300

ccgaggaccg gcgccugaag cggaccgcgc gccggcgcua cacccggcgc cggaaccgca      360

uccucuaccu gcaggagauc uucagcgagg agauggggaa ggucgacgac uccuucuucc      420

accggcugga ggacagcuuc cucgugacgg aggacaagcg cggcgagcgg caccccaucu      480

ucgggaaccu ggaggaggag gugaaguacc acgagaacuu cccgaccauc uaccaccucc      540

gccaguaccu ggccgacaac cccgagaagg ucgaccugcg gcugguguac cucgcccugg      600

cccacaucau caaguuccgc ggccacuucc ugaucgaggg gaaguucgac acccggaaca      660

acgacgugca gcgccucuuc caggaguucc uggcggucua cgacaacacc uucgagaacu      720

ccagccugca ggagcagaac guccaggugg aggagauccu cacggacaag aucuccaaga      780

gcgccaagaa ggaccgggug cugaagcugu uccccaacga gaaguccaac ggccgcuucg      840

ccgaguuccu caagcugauc gugggcaacc aggccgacuu caagaagcac uucgagcugg      900

aggagaaggc cccgcuccag uucagcaagg acaccuacga ggaggagcug gaggugcugc      960

ucgcgcagau cggggacaac uacgccgagc uguuccuguc cgccaagaag cucuacgaca     1020

gcauccugcu guccggcauc cucaccguca ccgacguggg gacgaaggcc ccgcugagcg     1080

ccuccaugau ccagcgguac aacgagcacc agauggaccu ggcgcagcuc aagcaguuca     1140

uccgccagaa gcugagcgac aaguacaacg agguguucuc cgacgucagc aaggacggcu     1200

acgccggcua caucgacggg aagaccaacc aggaggccuu cuacaaguac cugaagggcc     1260

uccugaacaa gaucgagggg agcggcuacu uccuggacaa gaucgagcgc gaggacuucc     1320

uccggaagca gcgcaccuuc gacaacgggu ccauccccca ccagauccac cuccaggaga     1380

ugcgggccau cauccgccgg caggccgagu ucuaccccuu ccuggcggac aaccaggacc     1440

gcaucgagaa gcugcugacc uuccggaucc ccuacuacgu gggcccgcug gcccgcggca     1500

agagcgacuu cgccuggcug ucccgcaaga gcgccgacaa gaucacgccc uggaacuucg     1560

acgagaucgu ggacaaggag uccagcgccg aggcguucau caaccggaug accaacuacg     1620

accucuaccu gcccaaccag aagguccugc ccaagcacuc ccuccuguac gagaaguuca     1680

ccguguacaa cgagcugacc aaggucaagu acaagacgga gcaggggaag accgccuucu     1740

ucgacgccaa caugaagcag gagaucuucg acggcguguu caagguguac cggaagguca     1800

ccaaggacaa gcucauggac uuccuggaga aggaguucga cgaguuccgc aucguggacc     1860

ugaccgggcu cgacaaggag aacaaggugu ucaacgccag cuacggcacg uaccacgacc     1920

ugugcaagau ccuggacaag gacuuccucg acaacuccaa gaacgagaag auccuggagg     1980

acaucguccu gacccucacc cuguucgagg accgggagau gauccgcaag cggcuggaga     2040

acuacagcga ccuccugacc aaggagcagg ucaagaagcu ggagcgccgg cacuacacgg     2100

gguggggccg ccucuccgcg gagcugaucc acggcauccg gaacaaggag agccgcaaga     2160

ccauccugga cuaccucauc gacgacggga acuccaaccg caacuucaug cagcugauca     2220

acgacgacgc ccugagcuuc aaggaggaga ucgccaaggc ccaggugauc ggcgagaccg     2280

acaaccucaa ccagguggug uccgacaucg ccgggagccc ggcgaucaag aagggcaucc     2340

ugcagucccu gaagaucgug gacgagcucg ucaagaucau ggggcaccag cccgagaaca     2400

ucguggugga gauggcccgg gagaaccagu ucaccaacca gggccgccgg aacagccagc     2460

agcgccugaa gggccugacg gacuccauca aggaguucgg gagccagauc cucaaggagc     2520

accccgucga gaacagccag cugcagaacg accggcuguu ccucuacuac cugcagaacg     2580

gccgcgacau guacaccggg gaggagcugg acaucgacua ccucucccag uacgacaucg     2640

accacaucau cccccaggcc uucaucaagg acaacagcau cgacaaccgg gugcugaccu     2700

ccagcaagga gaaccgcggc aaguccgacg acgugccgag caaggacguc gugcggaaga     2760

ugaaguccua cuggagcaag cugcucuccg ccaagcugau cacccagcgc aaguucgaca     2820

accugacgaa ggccgagcgg ggcgggcuca ccgacgacga caaggcgggc uucaucaagc     2880

gccagcuggu ggagacccgg cagaucacca agcacgucgc ccgcauccug gacgagcggu     2940

ucaacaccga gacggacgag aacaacaaga agauccgcca ggugaagauc gugacccuca     3000

agagcaaccu ggucuccaac uuccggaagg aguucgagcu guacaaggug cgcgagauca     3060

acgacuacca ccacgcccac gacgccuacc ucaacgccgu gaucgggaag gcgcugcugg     3120

gcgucuaccc ccagcucgag cccgaguucg uguacgggga cuacccccac uuccacggcc     3180

acaaggagaa caaggccacc gccaagaagu ucuucuacag caacaucaug aacuucuuca     3240

agaaggacga cgugcggacc gacaagaacg gcgagaucau cuggaagaag gacgagcaca     3300

ucuccaacau caagaagguc cugagcuacc cccaggugaa caucgugaag aaggucgagg     3360

agcagaccgg gggcuucucc aaggagagca uccugcccaa ggggaacucc gacaagcuca     3420

ucccccgcaa gacgaagaag uucuacuggg acaccaagaa guacggcggg uucgacagcc     3480

ccaucguggc cuacagcauc cuggugaucg cggacaucga gaagggcaag uccaagaagc     3540

ugaagaccgu caaggcccuc gugggcguga cgaucaugga gaagaugacc uucgagcggg     3600

acccggucgc cuuccuggag cgcaaggggu accgcaacgu gcaggaggag aacaucauca     3660

agcugcccaa guacagccuc uucaagcugg agaacggccg gaagcgccug cucgccuccg     3720

cccgggagcu gcagaagggg aacgagaucg ugcugcccaa ccaccucggc acccugcugu     3780

accacgcgaa gaacauccac aaggucgacg agccgaagca ccucgacuac guggacaagc     3840

acaaggacga guucaaggag cugcuggacg uggucagcaa cuucuccaag aaguacaccc     3900

ucgccgaggg gaaccuggag aagaucaagg agcuguacgc ccagaacaac ggcgaggacc     3960

ucaaggagcu ggccagcucc uucaucaacc ugcucacguu caccgccauc ggcgcgcccg     4020

ccaccuucaa guucuucgac aagaacaucg accgcaagcg guacaccagc acgaccgaga     4080

uccugaacgc cacccugauc caccagucca ucaccgggcu cuacgagacg cgcaucgacc     4140

ugaacaagcu gggcggggac aagcggcccg ccgccaccaa gaaggcgggc caggccaaga     4200

agaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu uuuugaaaua     4260

aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                                    4351


<210>  2403
<211>  3484
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2403
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgca gaccaccaac cugagcuaca uccucggccu ggaccugggg aucgccuccg      180

ugggcugggc cguggucgag aucaacgaga acgaggaccc caucgggcuc aucgacgugg      240

gcgugcggau cuucgagcgc gccgaggucc ccaagacggg cgagagccug gcccuguccc      300

ggcgccucgc gcggagcacc cgccggcuga uccgccggcg cgcccaccgg cugcuccugg      360

ccaagcgcuu ccugaagcgg gaggggaucc ucuccaccau cgaccuggag aagggccugc      420

ccaaccaggc cugggagcuc cgcguggcgg ggcuggagcg gcgccugagc gccaucgagu      480

ggggcgccgu gcuccugcac cugaucaagc accgggggua ccucuccaag cgcaagaacg      540

agagccagac caacaacaag gagcugggcg cccugcucuc cggcgucgcc cagaaccacc      600

agcugcugca gagcgacgac uaccggacgc cggcggagcu cgcccugaag aaguucgcca      660

aggaggaggg gcacauccgc aaccagcggg gcgccuacac ccacaccuuc aaccgccugg      720

accuccuggc cgagcugaac cuccuguucg cgcagcagca ccaguucggg aacccccacu      780

gcaaggagca cauccagcag uacaugaccg agcugcucau guggcagaag cccgcccugu      840

ccggcgaggc cauccugaag augcucggca agugcacgca cgagaagaac gaguucaagg      900

ccgccaagca caccuacagc gcggagcgcu ucguguggcu gaccaagcug aacaaccucc      960

ggauccugga ggacggggcc gagcgcgccc ugaacgagga ggagcggcag cuccugauca     1020

accaccccua cgagaagucc aagcugaccu acgcccaggu gcgcaagcuc cugggccuga     1080

gcgagcaggc caucuucaag caccuccggu acuccaagga gaacgcggag agcgccacgu     1140

ucauggagcu caaggccugg cacgccaucc gcaaggcgcu ggagaaccag gggcugaagg     1200

acaccuggca ggaccucgcc aagaagccgg accugcugga cgagaucggc accgccuucu     1260

cccucuacaa gaccgacgag gacauccagc aguaccugac gaacaagguc cccaacagcg     1320

ugaucaacgc ccugcucgug ucccugaacu ucgacaaguu caucgagcug agccucaaga     1380

gccugcggaa gauccugccc cucauggagc aggggaagcg cuacgaccag gccugccggg     1440

agaucuacgg ccaccacuac ggcgaggcga accagaagac cucccagcug cugcccgcca     1500

ucccggccca ggagauccgc aaccccgucg ugcuccggac ccugagccag gcccgcaagg     1560

ugaucaacgc caucauccgg caguacgggu cccccgcgcg cguccacauc gagaccggcc     1620

gggagcuggg caagagcuuc aaggagcgcc gggagaucca gaagcagcag gaggacaacc     1680

gcacgaagcg ggaguccgcc gugcagaagu ucaaggagcu guucagcgac uucuccagcg     1740

agcccaaguc caaggacauc cugaaguucc gccuguacga gcagcagcac gggaagugcc     1800

ucuacagcgg gaaggagauc aacauccacc gccugaacga gaagggcuac guggagaucg     1860

accacgcccu gccguucucc cggaccuggg acgacagcuu caacaacaag guccucgugc     1920

uggccuccga gaaccagaac aagggcaacc agacccccua cgaguggcug caggggaaga     1980

ucaacagcga gcgcuggaag aacuucgugg cccucguccu gggcucccag ugcagcgcgg     2040

ccaagaagca gcggcugcuc acccagguga ucgacgacaa caaguucauc gaccgcaacc     2100

ugaacgacac gcgguacauc gcccgcuucc uguccaacua cauccaggag aaccuccugc     2160

uggucgggaa gaacaagaag aacguguuca cccccaacgg ccagaucacc gcccuccugc     2220

ggagccgcug ggggcugauc aaggcgcggg agaacaacaa ccgccaccac gcccucgacg     2280

ccaucguggu cgccugcgcc acccccucca ugcagcagaa gaucacgcgg uucauccgcu     2340

ucaaggaggu gcacccguac aagaucgaga accgguacga gaugguggac caggagagcg     2400

gcgagaucau cagcccccac uuccccgagc ccugggcgua cuuccgccag gaggucaaca     2460

uccggguguu cgacaaccac ccggacaccg ugcugaagga gaugcugccc gaccgccccc     2520

aggccaacca ccaguucguc cagccccucu ucgugucccg ggccccgacc cgcaagauga     2580

gcggccaggg gcacauggag accaucaagu ccgccaagcg gcuggccgag ggcaucagcg     2640

ugcugcgcau cccccucacg cagcugaagc ccaaccugcu cgagaacaug gucaacaagg     2700

agcgggagcc ggcgcuguac gccgggcuga aggcccgccu cgccgaguuc aaccaggacc     2760

ccgccaaggc guucgccacc cccuucuaca agcagggcgg ccagcagguc aaggccaucc     2820

gcguggagca gguccagaag uccggggugc uggugcggga gaacaacggc guggccgaca     2880

acgcgagcau cgugcgcacc gacguguuca ucaagaacaa caaguucuuc cuggucccca     2940

ucuacaccug gcaggucgcc aaggggaucc ucccgaacaa ggccaucgug gcccacaaga     3000

acgaggacga gugggaggag auggacgagg gcgccaaguu caaguucucc cuguucccca     3060

acgaccuggu ggagcucaag acgaagaagg aguacuucuu cggguacuac aucggccugg     3120

accgggcgac cggcaacauc agccugaagg agcacgacgg ggagaucucc aagggcaagg     3180

acggggugua ccgcgugggc gucaagcucg cccugagcuu cgagaaguac cagguggacg     3240

agcuggggaa gaaccggcag aucugccgcc cccagcagcg gcagcccgug cgcaagcggc     3300

cggccgccac caagaaggcc ggccaggcga agaagaagaa guaaggacua gucccuguuc     3360

ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca gaucuaaaaa     3420

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag     3480

aauu                                                                  3484


<210>  2404
<211>  3562
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2404
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc cgccuucaag cccaacccca ucaacuacau ccugggccuc gacaucggga      180

ucgccagcgu gggcugggcc augguggaga ucgacgagga cgagaacccc aucugccuga      240

ucgaccuggg gguccgggug uucgagcgcg cggaggugcc gaagaccggc gacucccucg      300

ccauggcccg gcgccuggcc cggagcgucc gccggcugac ccgccggcgc gcgcaccggc      360

uccugcgcgc ccggcgccug cucaagcggg agggcgugcu gcaggccgcc gacuucgacg      420

agaacgggcu gaucaagucc cuccccaaca cgcccuggca gcugcgcgcc gcggcccugg      480

accggaagcu cacgccccug gaguggagcg ccgugcugcu ccaccugauc aagcaccgcg      540

gcuaccuguc ccagcggaag aacgaggggg agaccgccga caaggagcuc ggcgcccugc      600

ugaagggggu cgcggacaac gcccacgccc uccagaccgg cgacuuccgc accccggccg      660

agcuggcccu gaacaaguuc gagaaggaga gcggccacau ccggaaccag cgcggggacu      720

acucccacac cuucagccgc aaggaccucc aggcggagcu gauccuccuc uucgagaagc      780

agaaggaguu cggcaacccc cacguguccg ggggccugaa ggagggcauc gagacccugc      840

ucaugaccca gcggcccgcc cugagcgggg acgccgugca gaagaugcug ggccacugca      900

cguucgagcc cgccgagccg aaggccgcga agaacaccua caccgccgag cgcuucaucu      960

ggcucaccaa gcugaacaac cugcggaucc ucgagcaggg guccgagcgc ccccugacgg     1020

acaccgagcg ggccacccug auggacgagc ccuaccgcaa gagcaagcuc accuacgccc     1080

aggcgcggaa gcugcugggc cucgaggaca cggccuucuu caaggggcug cgcuacggca     1140

aggacaacgc cgaggccucc acccugaugg agaugaaggc cuaccacgcg aucagccggg     1200

cccucgagaa ggagggccug aaggacaaga aguccccgcu gaaccugagc cccgagcucc     1260

aggacgagau cgggaccgcc uucucccugu ucaagaccga cgaggacauc acgggccgcc     1320

ugaaggaccg gauccagccc gagauccucg aggcccugcu gaagcacauc agcuucgaca     1380

aguucgucca gaucucccuc aaggcccugc gccggaucgu gccccugaug gagcagggga     1440

agcgcuacga cgaggcgugc gccgagaucu acggcgacca cuacgggaag aagaacaccg     1500

aggagaagau cuaccugccc ccgauccccg ccgacgagau ccggaacccc gugguccugc     1560

gcgcccugag ccaggcccgg aaggugauca acggcguggu ccgccgguac ggcagccccg     1620

cgcgcaucca caucgagacc gcccgcgagg uggggaaguc cuucaaggac cggaaggaga     1680

ucgagaagcg ccaggaggag aaccggaagg accgcgagaa ggccgccgcg aaguuccggg     1740

aguacuuccc gaacuucgug ggcgagccca agagcaagga cauccucaag cugcgccugu     1800

acgagcagca gcacgggaag ugccucuacu ccggcaagga gaucaaccug gggcggcuga     1860

acgagaaggg cuacgucgag aucgaccacg cccuccccuu cagccgcacc ugggacgacu     1920

ccuucaacaa caaggugcug gugcugggca gcgagaacca gaacaagggg aaccagacgc     1980

ccuacgagua cuucaacggc aaggacaacu cccgggagug gcaggaguuc aaggcccgcg     2040

ucgagaccag ccgguucccg cgcuccaaga agcagcggau ccuccugcag aaguucgacg     2100

aggacggguu caaggagcgc aaccugaacg acacccggua cgugaaccgc uuccucugcc     2160

aguucgucgc cgaccggaug cgccugaccg gcaagggcaa gaagcgggug uucgccagca     2220

acgggcagau cacgaaccug cuccgcggcu ucugggggcu gcggaaggug cgcgcggaga     2280

acgaccgcca ccacgcccug gacgccgucg ugguggccug cuccaccguc gccaugcagc     2340

agaagaucac ccgguucgug cgcuacaagg agaugaacgc guucgacggc aagaccaucg     2400

acaaggagac gggggaggug cuccaccaga agacccacuu cccccagccc ugggaguucu     2460

ucgcccagga ggucaugauc cggguguucg gcaagccgga cggcaagccc gaguucgagg     2520

aggccgacac ccccgagaag cugcgcaccc ugcucgccga gaagcugagc ucccggcccg     2580

aggccgugca cgaguacguc acgccgcugu ucgugagccg cgcgcccaac cggaagaugu     2640

ccgggcaggg ccacauggag accgucaaga gcgccaagcg ccucgacgag gggguguccg     2700

ugcugcgggu gccccugacc cagcucaagc ugaaggaccu ggagaagaug gucaaccgcg     2760

agcgggagcc caagcucuac gaggcccuga aggcccgccu ggaggcgcac aaggacgacc     2820

cggccaaggc cuucgccgag cccuucuaca aguacgacaa ggccggcaac cggacccagc     2880

aggucaaggc ggugcgcgug gagcaggucc agaagaccgg ggugugggug cggaaccaca     2940

acggcaucgc cgacaacgcc acgauggugc gcguggacgu cuucgagaag ggcgacaagu     3000

acuaccucgu gcccaucuac agcuggcagg ucgccaaggg gauccugccc gaccgggccg     3060

uggugcaggg caaggacgag gaggacuggc agcugaucga cgacuccuuc aacuucaagu     3120

ucagccucca cccgaacgac cugguggagg ucaucaccaa gaaggcgcgc auguucgggu     3180

acuucgccag cugccaccgg ggcaccggga acaucaacau ccgcauccac gaccuggacc     3240

acaagaucgg caagaacggc auccucgagg ggaucggcgu gaagaccgcc cuguccuucc     3300

agaaguacca gaucgacgag cuggggaagg agauccgccc cugccggcuc aagaagcgcc     3360

cgcccgugcg gaagcgcccc gccgccacga agaaggcggg ccaggccaag aagaagaagu     3420

aaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu     3480

gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaagaa uu                                              3562


<210>  2405
<211>  3568
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2405
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa guaccacgug ggcaucgacg uggggaccuu cagcgucggc cuggccgcca      180

ucgaggugga cgacgccggg augcccauca agacccuguc ccuggugagc cacauccacg      240

acuccggccu ggaccccgac gagaucaaga gcgccgucac gcggcucgcg uccagcggca      300

ucgcccgccg gacccgccgg cuguaccgcc ggaagcgccg gcgccugcag cagcucgaca      360

aguucaucca gcggcagggg uggcccguga ucgagcugga ggacuacucc gacccgcugu      420

accccuggaa ggugcgcgcc gagcucgccg cgagcuacau cgccgacgag aaggagcggg      480

gcgagaagcu guccgucgcc cugcgccaca ucgcccggca ccgcgggugg cggaaccccu      540

acgccaaggu gagcucccuc uaccugcccg acggcccgag cgacgcguuc aaggccaucc      600

gcgaggagau caagcgggcc uccgggcagc ccgugcccga gaccgccacc gucggccaga      660

uggugacgcu gugcgagcuc ggcacccuga agcugcgcgg ggagggcggg gugcucagcg      720

cccggcugca gcaguccgac uacgcgcgcg agauccagga gaucugccgc augcaggaga      780

ucggccagga gcuguaccgg aagaucaucg acgucguguu cgccgccgag agccccaagg      840

gcuccgccag cucccgcgug gggaaggacc cgcuccagcc cggcaagaac cgggcccuga      900

aggcgagcga cgccuuccag cgcuaccgga ucgccgcccu gaucgggaac cuccgcgucc      960

ggguggacgg cgagaagcgc auccugagcg uggaggagaa gaaccugguc uucgaccacc     1020

ucgugaaccu gacccccaag aaggagcccg agugggugac caucgccgag auccugggga     1080

ucgaccgggg ccagcucauc ggcacggcga ccaugaccga cgacggggag cgcgccggcg     1140

cccggccgcc cacccacgac acgaaccgcu ccaucgucaa cagccggauc gcgccccugg     1200

uggacuggug gaagaccgcc uccgcccugg agcagcacgc cauggucaag gcccucagca     1260

acgccgaggu ggacgacuuc gacucccccg agggggcgaa ggugcaggcc uucuucgccg     1320

accuggacga cgacguccac gccaagcugg acagccucca ccucccggug ggccgcgccg     1380

cguacuccga ggacacccug gugcggcuca cccgccggau gcugagcgac ggggucgacc     1440

uguacacggc ccgccuccag gaguucggca ucgagcccuc cuggaccccg cccacgcccc     1500

ggaucggcga gcccgugggg aaccccgccg uggaccgcgu ccugaagacc gugagccgcu     1560

ggcuggaguc cgccaccaag accuggggcg cccccgagcg ggugaucauc gagcacgucc     1620

gcgagggguu cgugaccgag aagcgggcgc gcgagaugga cggcgacaug cggcgccggg     1680

ccgcccgcaa cgccaagcug uuccaggaga ugcaggagaa gcugaacgug caggggaagc     1740

cgagccgggc ggaccugugg cgcuaccagu ccguccagcg gcagaacugc cagugcgccu     1800

acugcggcag ccccaucacc uucuccaaca gcgagaugga ccacaucgug ccccgcgccg     1860

gccagggguc cacgaacacc cgggagaacc ucguggccgu cugccaccgc ugcaaccaga     1920

gcaagggcaa caccccguuc gccaucuggg cgaagaacac cuccaucgag ggggugagcg     1980

ugaaggaggc cgucgagcgg acgcgccacu gggugaccga caccggcaug cggagcaccg     2040

acuucaagaa guucacgaag gccguggucg agcgcuucca gcgggccacc auggacgagg     2100

agaucgacgc ccgcuccaug gagagcgugg cguggauggc caacgagcug cggucccgcg     2160

uggcccagca cuucgccagc cacggcacca ccguccgcgu guaccggggg ucccugacgg     2220

ccgaggcgcg ccgggccagc ggcaucuccg ggaagcugaa guucuucgac ggcgugggca     2280

agagccgccu cgaccggcgc caccacgcca ucgacgccgc ggucaucgcc uucaccuccg     2340

acuacguggc cgagacccug gccgugcgga gcaaccugaa gcagucccag gcccaccgcc     2400

aggaggcgcc ccaguggcgg gaguucaccg ggaaggacgc cgagcaccgc gccgccuggc     2460

gggucuggug ccagaagaug gagaagcuca gcgcccugcu gaccgaggac cuccgcgacg     2520

accggguggu ggucaugucc aacgugcgcc ugcggcuggg caacgggagc gcgcacaagg     2580

agacgaucgg caagcucucc aaggugaagc ugagcuccca gcugagcguc agcgacaucg     2640

acaaggccuc cagcgaggcc cucuggugcg cccugacccg cgagcccggg uucgacccca     2700

aggagggccu gccggccaac cccgagcggc acauccgcgu gaacgggacc cacguguacg     2760

cgggcgacaa caucggccuc uuccccgucu ccgccgggag caucgcccug cggggcgggu     2820

acgccgagcu gggcuccagc uuccaccacg cccgcgugua caagaucacc uccgggaaga     2880

agcccgcguu cgccaugcuc cgcguguaca cgaucgaccu gcugccguac cggaaccagg     2940

accucuucag cgucgagcug aagccccaga ccauguccau gcgccaggcc gagaagaagc     3000

ugcgggacgc ccucgcgacc ggcaacgccg aguaccuggg cuggcuggug guggacgacg     3060

agcucgucgu ggacaccagc aagaucgcca cggaccaggu gaaggccguc gaggccgagc     3120

uggggaccau ccgccggugg cgcguggacg gcuucuucuc ccccagcaag cugcggcucc     3180

gcccccugca gauguccaag gaggggauca agaaggagag cgcgccggag cuguccaaga     3240

ucaucgaccg gcccggcugg cuccccgccg ucaacaagcu guucagcgac gggaacguga     3300

ccgugguccg ccgggacucc cugggccgcg ugcggcucga gagcaccgcc caccuccccg     3360

ugacguggaa gguccagaag cgcccggccg ccaccaagaa ggcgggccag gccaagaaga     3420

agaaguaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa     3480

uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        3568


<210>  2406
<211>  3268
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2406
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc ccggauccug gccuucgaca ucggcaucag cuccaucggg ugggccuuca      180

gcgagaacga cgagcucaag gacugcggcg ugcgcaucuu caccaaggug gagaacccca      240

agaccgggga gucccuggcc cugccccggc gccucgcgcg gagcgcccgc aagcggcugg      300

cccgccggaa ggcccgccug aaccaccuca agcaccugau cgcgaacgag uucaagcuga      360

acuacgagga cuaccagucc uucgacgaga gccucgccaa ggccuacaag ggcucccuga      420

ucagccccua cgagcugcgg uuccgcgccc ucaacgagcu gcuguccaag caggacuucg      480

cccgggucau ccuccacauc gcgaagcgcc ggggcuacga cgacaucaag aacagcgacg      540

acaaggagaa gggggccauc cugaaggcca ucaagcagaa cgaggagaag cuggccaacu      600

accaguccgu gggcgaguac cucuacaagg aguacuucca gaaguucaag gagaacagca      660

aggaguucac gaacgugcgc aacaagaagg aguccuacga gcggugcauc gcccagagcu      720

uccugaagga cgagcugaag cucaucuuca agaagcagcg cgaguucggg uucuccuuca      780

gcaagaaguu cgaggaggag guccuguccg uggcguucua caagcgggcc cugaaggacu      840

ucagccaccu cgugggcaac ugcuccuucu ucaccgacga gaagcgcgcc ccgaagaaca      900

gcccccuggc cuucauguuc gucgcccuga cccggaucau caaccuccug aacaaccuga      960

agaacaccga ggggauccuc uacacgaagg acgaccugaa cgcgcugcuc aacgaggugc     1020

ugaagaacgg cacccugacc uacaagcaga ccaagaagcu ccugggccug agcgacgacu     1080

acgaguucaa gggggagaag ggcacguacu ucaucgaguu caagaaguac aaggaguuca     1140

ucaaggcccu cggggagcac aaccuguccc aggacgaccu gaacgagauc gccaaggaca     1200

ucacccucau caaggacgag aucaagcuga agaaggcccu ggccaaguac gaccucaacc     1260

agaaccagau cgacagccug uccaagcugg aguucaagga ccaccucaac aucagcuuca     1320

aggcgcugaa gcuggugacg ccccugaugc ucgagggcaa gaaguacgac gaggccugca     1380

acgagcugaa ccugaagguc gccaucaacg aggacaagaa ggacuuccuc cccgccuuca     1440

acgagaccua cuacaaggac gaggugacca acccgguggu ccugcgcgcg aucaaggagu     1500

accgcaaggu gcugaacgcc cuccugaaga aguacggcaa ggugcacaag aucaacaucg     1560

agcuggcccg ggaggucggg aagaaccacu cccagcgcgc caagaucgag aaggagcaga     1620

acgagaacua caaggccaag aaggacgcgg agcucgagug cgagaagcug ggccugaaga     1680

ucaacagcaa gaacauccuc aagcugcggc uguucaagga gcagaaggag uucugcgccu     1740

acuccgggga gaagaucaag aucagcgacc uccaggacga gaagaugcug gagaucgacc     1800

acaucuaccc cuacucccgc agcuucgacg acuccuacau gaacaaggug cugguguuca     1860

ccaagcagaa ccaggagaag cucaaccaga cccccuucga ggccuucggc aacgacagcg     1920

ccaaguggca gaagaucgag guccuggcca agaaccugcc caccaagaag cagaagcgga     1980

uccucgacaa gaacuacaag gacaaggagc agaagaacuu caaggaccgc aaccugaacg     2040

acacgcggua caucgcgcgc cuggugcuca acuacaccaa ggacuaccug gacuuccugc     2100

cgcucuccga cgacgagaac accaagcuga acgacaccca gaaggggagc aaggugcacg     2160

ucgaggccaa guccggcaug cugacgagcg cccuccggca caccuggggc uucuccgcca     2220

aggaccgcaa caaccaccuc caccacgcca ucgacgcggu gaucaucgcc uacgccaaca     2280

acagcaucgu caaggccuuc uccgacuuca agaaggagca ggagagcaac agcgcggagc     2340

uguacgccaa gaagaucucc gagcuggacu acaagaacaa gcggaaguuc uucgagcccu     2400

ucagcggguu ccgccagaag gugcuggaca agaucgacga gaucuucgug uccaagcccg     2460

agcggaagaa gcccagcggc gcccugcacg aggagaccuu ccgcaaggag gaggaguucu     2520

accaguccua cgggggcaag gagggggucc ucaaggcccu ggagcugggc aagauccgga     2580

aggugaacgg caagaucgug aagaacgggg acauguuccg cgucgacauc uucaagcaca     2640

agaagaccaa caaguucuac gccgugccga ucuacacgau ggacuucgcg cucaaggugc     2700

ugcccaacaa ggccgucgcc cggagcaaga agggcgagau caaggacugg auccugaugg     2760

acgagaacua cgaguucugc uucucccucu acaaggacag ccugauccug auccagacca     2820

aggacaugca ggagcccgag uucguguacu acaacgccuu caccuccagc accguguccc     2880

ucaucgucag caagcacgac aacaaguucg agacgcuguc caagaaccag aagauccugu     2940

ucaagaacgc caacgagaag gaggugaucg ccaagagcau cgggauccag aaccucaagg     3000

uguucgagaa guacaucguc uccgcgcugg gcgaggugac caaggccgag uuccgccagc     3060

gggaggacuu caagaagaag cgccccgccg ccaccaagaa ggcggggcag gccaagaaga     3120

agaaguaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa     3180

uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        3268


<210>  2407
<211>  3679
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2407
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgag cgaccuggug cucggccugg acaucgggau cggcuccgug ggggucggca      180

uccugaacaa ggugaccggc gagaucaucc acaagaacag ccggaucuuc cccgccgccc      240

aggccgagaa caaccucgug cgccggacca accgccaggg gcggcgccug gcccggcgca      300

agaagcaccg gcgcguccgg cugaaccgcc ucuucgagga guccggccug aucacggacu      360

ucaccaagau cagcaucaac cugaaccccu accagcuccg ggugaagggg cugaccgacg      420

agcuguccaa cgaggagcug uucaucgcgc ugaagaacau ggugaagcac cgcggcauca      480

gcuaccugga cgacgccucc gacgacggga acagcuccgu cggcgacuac gcccagaucg      540

ugaaggagaa cagcaagcag cucgagacca agacgcccgg ccagauccag cuggagcggu      600

accagaccua cgggcagcug cgcggcgacu ucaccgugga gaaggacggg aagaagcacc      660

ggcucaucaa cgucuucccg accuccgccu accgcagcga ggcgcugcgg auccugcaga      720

cgcagcagga guucaacccc cagaucaccg acgaguucau caaccgcuac cucgagaucc      780

ugaccggcaa gcggaaguac uaccacggcc ccgggaacga gaagucccgc accgacuacg      840

gccgcuaccg gacgagcggg gagacccugg acaacaucuu cggcauccuc aucgggaagu      900

gcaccuucua ccccgacgag uuccgcgccg ccaaggccuc cuacaccgcc caggaguuca      960

accugcugaa cgaccucaac aaccugacgg ugccgaccga gaccaagaag cugagcaagg     1020

agcagaagaa ccagaucauc aacuacguga agaacgagaa ggcgaugggc cccgccaagc     1080

uguucaagua caucgccaag cuccuguccu gcgacgucgc cgacaucaag ggcuaccgga     1140

ucgacaagag cgggaaggcc gagauccaca ccuucgaggc guaccgcaag augaagacgc     1200

ucgagacccu ggacaucgag cagauggacc gggagacccu ggacaagcuc gccuacgugc     1260

ugacccugaa cacggagcgc gagggcaucc aggaggcccu cgagcacgag uucgccgacg     1320

gguccuucag ccagaagcag gucgacgagc uggugcaguu ccggaaggcc aacagcucca     1380

ucuucggcaa gggguggcac aacuucagcg ugaagcugau gauggagcuc auccccgagc     1440

uguacgagac cuccgaggag cagaugacca uccugacccg ccucggcaag cagaagacga     1500

ccagcuccag caacaagacc aaguacaucg acgagaagcu gcugaccgag gagaucuaca     1560

accccguggu cgcgaagucc gugcggcagg ccaucaagau cgugaacgcc gccaucaagg     1620

aguacggcga cuucgacaac aucgucaucg agauggcccg cgagacgaac gaggacgacg     1680

agaagaaggc gauccagaag auccagaagg ccaacaagga cgagaaggac gccgccaugc     1740

ucaaggcggc caaccaguac aacgggaagg ccgagcugcc gcacagcgug uuccacggcc     1800

acaagcagcu ggccaccaag auccggcugu ggcaccagca gggggagcgc ugccucuaca     1860

ccggcaagac caucuccauc cacgaccuga ucaacaacag caaccaguuc gaggucgacc     1920

acauccugcc ccucuccauc acguucgacg acagccuggc caacaaggug cugguguacg     1980

cgaccgccaa ccaggagaag gggcagcgga cccccuacca ggcccucgac uccauggacg     2040

acgccuggag cuuccgcgag cugaaggccu ucguccggga guccaagacc cugagcaaca     2100

agaagaagga guaccuccug accgaggagg acaucuccaa guucgacgug cgcaagaagu     2160

ucaucgagcg gaaccuggug gacacgcgcu acgcgagccg ggucgugcuc aacgcccugc     2220

aggagcacuu ccgcgcccac aagaucgaca ccaagguguc cgucgugcgc ggccaguuca     2280

ccagccagcu gcggcgccac uggggcaucg agaagacccg ggacacguac caccaccacg     2340

ccguggacgc ccucaucauc gcggccagcu cccagcugaa ccuguggaag aagcagaaga     2400

acacccucgu cagcuacucc gaggaccagc ugcuggacau cgagaccggg gagcucauca     2460

gcgacgacga guacaaggag uccguguuca aggcccccua ccagcacuuc guggacaccc     2520

ucaagagcaa ggaguucgag gacuccaucc uguucagcua ccaggucgac uccaaguuca     2580

accgcaagau cagcgacgcc acgaucuacg cgacccggca ggccaaggug ggcaaggaca     2640

aggccgacga gaccuacgug cucgggaaga ucaaggacau cuacacccag gacggcuacg     2700

acgccuucau gaagaucuac aagaaggaca aguccaaguu ccugauguac cgccacgacc     2760

cgcagacguu cgagaagguc aucgagccca uccuggagaa cuaccccaac aagcagauca     2820

acgagaaggg caaggaggug cccugcaacc cguuccucaa guacaaggag gagcacgggu     2880

acauccggaa guacagcaag aagggcaacg ggcccgagau caagucccug aaguacuacg     2940

acagcaagcu gggcaaccac aucgacauca cccccaagga cuccaacaac aagguggucc     3000

uccagagcgu gucccccugg cgcgccgacg uguacuucaa caagaccacc gggaaguacg     3060

agauccuggg ccugaaguac gcggaccucc aguucgagaa gggcacgggg accuacaaga     3120

ucagccagga gaaguacaac gacaucaaga agaaggaggg cgucgacagc gacuccgagu     3180

ucaaguucac ccuguacaag aacgaccugc uccuggugaa ggacaccgag acgaaggagc     3240

agcagcuguu ccgguuccuc agccgcacca ugccgaagca gaagcacuac guggagcuga     3300

agcccuacga caagcagaag uucgagggcg gggaggcccu gaucaagguc cucgggaacg     3360

uggccaacuc cggccagugc aagaagggcc ugggcaagag caacaucucc aucuacaagg     3420

ugcggaccga cguccugggg aaccagcaca ucaucaagaa cgagggggac aagcccaagc     3480

ucgacuucaa gcgccccgcc gccaccaaga aggcgggcca ggccaagaag aagaaguaag     3540

gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc     3600

uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaagaauu                                                  3679


<210>  2408
<211>  4237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2408
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgac ccaguucgag ggcuucacca accuguacca ggugagcaag acgcuccggu      180

ucgagcugau cccccagggg aagacccuga agcacaucca ggagcagggc uucaucgagg      240

aggacaaggc ccgcaacgac cacuacaagg agcucaagcc caucaucgac cggaucuaca      300

agaccuacgc cgaccagugc cugcagcugg ugcagcucga cugggagaac cuguccgccg      360

ccaucgacag cuaccgcaag gagaagaccg aggagacgcg gaacgcgcug aucgaggagc      420

aggccaccua ccgcaacgcc auccacgacu acuucaucgg gcggaccgac aaccucaccg      480

acgccaucaa caagcgccac gcggagaucu acaagggccu guucaaggcc gagcuguuca      540

acggcaaggu ccucaagcag cuggggacgg ugaccaccac cgagcacgag aacgcccugc      600

uccgguccuu cgacaaguuc acgaccuacu ucagcggcuu cuacgagaac cgcaagaacg      660

uguucuccgc cgaggacauc agcaccgcca ucccccaccg gaucguccag gacaacuucc      720

cgaaguucaa ggagaacugc cacaucuuca cccgccugau cacggcggug cccucccugc      780

gggagcacuu cgagaacgug aagaaggcca ucgggaucuu cgucagcacc uccaucgagg      840

agguguucag cuuccccuuc uacaaccagc uccugaccca gacccagauc gaccuguaca      900

accagcuccu gggcgggauc ucccgcgagg ccggcacgga gaagaucaag ggccugaacg      960

aggugcucaa ccuggccauc cagaagaacg acgagaccgc ccacaucauc gcgagccugc     1020

cccaccgguu caucccccuc uucaagcaga uccuguccga ccgcaacacc cugagcuuca     1080

uccucgagga guucaagucc gacgaggagg ucauccagag cuucugcaag uacaagaccc     1140

ugcugcggaa cgagaacgug cucgagacgg ccgaggcccu guucaacgag cugaacucca     1200

ucgaccucac ccacaucuuc aucagccaca agaagcugga gaccaucucc agcgcccugu     1260

gcgaccacug ggacacccuc cgcaacgccc uguacgagcg gcgcaucagc gagcugacgg     1320

ggaagaucac caaguccgcg aaggagaagg ugcagcggag ccucaagcac gaggacauca     1380

accugcagga gaucaucucc gccgccggca aggagcugag cgaggccuuc aagcagaaga     1440

ccuccgagau ccucagccac gcccacgcgg cccuggacca gccgcugccc accacgcuca     1500

agaagcagga ggagaaggag auccugaagu cccagcugga cagccugcuc gggcuguacc     1560

accuccucga cugguucgcc gucgacgagu ccaacgaggu ggaccccgag uucagcgccc     1620

gccugaccgg caucaagcug gagauggagc cgucccucag cuucuacaac aaggcgcgca     1680

acuacgccac caagaagccc uacuccgugg agaaguucaa gcugaacuuc cagaugccca     1740

cccuggccag cggcugggac gucaacaagg agaagaacaa cggggccauc cucuucguga     1800

agaacggccu guacuaccug gggaucaugc ccaagcagaa gggccgguac aaggcccucu     1860

ccuucgagcc gacggagaag accagcgagg gguucgacaa gauguacuac gacuacuucc     1920

ccgacgcggc caagaugauc cccaagugcu ccacccagcu gaaggccgug accgcccacu     1980

uccagaccca cacgaccccc auccugcuca gcaacaacuu caucgagccg cuggagauca     2040

ccaaggagau cuacgaccug aacaaccccg agaaggagcc caagaaguuc cagaccgccu     2100

acgcgaagaa gacgggcgac cagaagggcu accgcgaggc ccucugcaag uggaucgacu     2160

ucacccggga cuuccugucc aaguacacca agaccacgag caucgaccug agcucccucc     2220

gccccagcuc ccaguacaag gaccuggggg aguacuacgc cgagcugaac ccgcuccugu     2280

accacaucag cuuccagcgg aucgccgaga aggagaucau ggacgccguc gagaccggca     2340

agcuguaccu cuuccagauc uacaacaagg acuucgcgaa ggggcaccac ggcaagccca     2400

accugcacac ccuguacugg accggccucu ucucccccga gaaccuggcc aagacgagca     2460

ucaagcugaa cgggcaggcc gagcuguucu accgccccaa gucccggaug aagcgcaugg     2520

cccaccggcu gggcgagaag augcugaaca agaagcucaa ggaccagaag accccgaucc     2580

ccgacacccu guaccaggag cuguacgacu acgugaacca ccgccucagc cacgaccugu     2640

ccgacgaggc gcgggcccug cuccccaacg ugaucaccaa ggaggucagc cacgagauca     2700

ucaaggaccg ccgguucacg uccgacaagu ucuucuucca cgugccgauc acccugaacu     2760

accaggccgc caacagcccc uccaaguuca accagcgcgu caacgccuac cugaaggagc     2820

accccgagac ccccaucauc gggaucgacc ggggcgagcg caaccucauc uacaucaccg     2880

ugaucgacag cacggggaag auccuggagc agcggucccu gaacaccauc cagcaguucg     2940

acuaccagaa gaagcucgac aaccgcgaga aggagcgggu ggcggcccgc caggccugga     3000

gcgucguggg caccaucaag gaccugaagc agggguaccu gucccaggug auccacgaga     3060

ucgucgaccu caugauccac uaccaggccg uggugguccu ggagaaccug aacuucggcu     3120

ucaagagcaa gcgcaccggc aucgccgaga aggcggugua ccagcaguuc gagaagaugc     3180

ucaucgacaa gcugaacugc cuggugcuca aggacuaccc ggccgagaag gucgggggcg     3240

ugcugaaccc cuaccagcug acggaccagu ucaccagcuu cgccaagaug gggacccagu     3300

ccggcuuccu cuucuacgug cccgcccccu acaccagcaa gaucgacccg cugacgggcu     3360

ucgucgaccc cuucgugugg aagaccauca agaaccacga gucccggaag cacuuccugg     3420

agggguucga cuuccuccac uacgacguga agaccggcga cuucauccug cacuucaaga     3480

ugaaccgcaa ccugagcuuc cagcgggggc uccccggcuu caugcccgcc ugggacaucg     3540

ucuucgagaa gaacgagacc caguucgacg cgaaggggac gccguucauc gccggcaagc     3600

gcaucgugcc cgugaucgag aaccaccggu ucaccggccg cuaccgggac cuguaccccg     3660

ccaacgagcu gaucgcccuc cuggaggaga aggggaucgu cuuccgcgac ggcuccaaca     3720

uccugcccaa gcuccuggag aacgacgaca gccacgcgau cgacaccaug guggcccuga     3780

uccgguccgu gcuccagaug cgcaacagca acgccgccac cggggaggac uacaucaacu     3840

ccccgguccg ggaccugaac ggcgugugcu ucgacagccg cuuccagaac cccgaguggc     3900

ccauggacgc cgacgcgaac ggggccuacc acaucgcccu gaagggccag cuccugcuga     3960

accaccucaa ggaguccaag gaccugaagc ugcagaacgg caucagcaac caggacuggc     4020

ucgccuacau ccaggagcug cggaacaagc gccccgccgc gacgaagaag gccgggcagg     4080

ccaagaagaa gaaguaagga cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu     4140

gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                              4237


<210>  2409
<211>  4216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2409
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgag caucuaccag gaguucguga acaaguacuc ccugagcaag acccuccggu      180

ucgagcugau cccccagggc aagacccugg agaacaucaa ggcccgcggg cucauccugg      240

acgacgagaa gcgggccaag gacuacaaga aggccaagca gaucaucgac aaguaccacc      300

aguucuucau cgaggagauc cuguccagcg ugugcaucuc cgaggaccuc cugcagaacu      360

acagcgacgu cuacuucaag cugaagaagu ccgacgacga caaccuccag aaggacuuca      420

agagcgccaa ggacacgauc aagaagcaga ucuccgagua caucaaggac agcgagaagu      480

ucaagaaccu guucaaccag aaccugaucg acgcgaagaa gggccaggag uccgaccuca      540

uccuguggcu gaagcagagc aaggacaacg ggaucgagcu guucaaggcc aacuccgaca      600

ucaccgacau cgacgaggcc cucgagauca ucaagagcuu caagggcugg accaccuacu      660

ucaagggcuu ccacgagaac cgcaagaacg uguacuccag caacgacauc cccacgucca      720

ucaucuaccg gaucguggac gacaaccugc ccaaguuccu cgagaacaag gccaaguacg      780

agagccugaa ggacaaggcg ccggaggcca ucaacuacga gcagaucaag aaggaccugg      840

ccgaggagcu caccuucgac aucgacuaca agaccuccga ggucaaccag cgcguguuca      900

gccuggacga gguguucgag aucgccaacu ucaacaacua ccugaaccag agcgggauca      960

ccaaguucaa cacgaucauc ggcgggaagu ucgucaacgg cgagaacacc aagcggaagg     1020

ggaucaacga guacaucaac cucuacuccc agcagaucaa cgacaagacc cugaagaagu     1080

acaagaugag cgugcuguuc aagcagaucc ucuccgacac cgagagcaag uccuucguga     1140

ucgacaagcu ggaggacgac agcgacgucg ugacgaccau gcaguccuuc uacgagcaga     1200

ucgccgcguu caagaccgug gaggagaagu ccaucaagga gacccugucc cuccuguucg     1260

acgaccugaa ggcccagaag cucgaccuga gcaagaucua cuucaagaac gacaaguccc     1320

ugacggaccu cagccagcag gucuucgacg acuacuccgu gaucggcacc gccgugcugg     1380

aguacaucac ccagcagauc gcccccaaga accuggacaa ccccagcaag aaggagcagg     1440

agcucaucgc caagaagacc gagaaggcga aguaccuguc ccuggagacg aucaagcucg     1500

cccuggagga guucaacaag caccgcgaca ucgacaagca gugccgguuc gaggagaucc     1560

uggccaacuu cgccgccauc cccaugaucu ucgacgagau cgcgcagaac aaggacaacc     1620

ucgcccagau cagcaucaag uaccagaacc agggcaagaa ggaccugcug caggccuccg     1680

ccgaggacga cgucaaggcc aucaaggacc uccuggacca gaccaacaac cugcugcaca     1740

agcucaagau cuuccacauc agccaguccg aggacaaggc gaacauccug gacaaggacg     1800

agcacuucua ccugguguuc gaggagugcu acuucgagcu cgccaacauc gugccgcugu     1860

acaacaagau ccgcaacuac aucacccaga agcccuacag cgacgagaag uucaagcuga     1920

acuucgagaa cagcacccuc gccaacgggu gggacaagaa caaggagccc gacaacacgg     1980

ccauccuguu caucaaggac gacaaguacu accugggcgu caugaacaag aagaacaaca     2040

agaucuucga cgacaaggcg aucaaggaga acaaggggga gggcuacaag aagaucgugu     2100

acaagcuccu gcccggcgcc aacaagaugc ugccgaaggu guucuucucc gccaagagca     2160

ucaaguucua caaccccucc gaggacaucc uccggauccg caaccacagc acccacacca     2220

agaacgggag cccccagaag ggcuacgaga aguucgaguu caacaucgag gacugccgga     2280

aguucaucga cuucuacaag cagagcaucu ccaagcaccc cgaguggaag gacuucgggu     2340

uccgcuucag cgacacccag cgguacaacu ccaucgacga guucuaccgc gaggucgaga     2400

accagggcua caagcugacg uucgagaaca ucagcgaguc cuacaucgac agcgugguca     2460

accaggggaa gcuguaccuc uuccagaucu acaacaagga cuucuccgcc uacagcaagg     2520

gccggccgaa ccugcacacc cuguacugga aggcccucuu cgacgagcgc aaccugcagg     2580

acguggugua caagcugaac ggcgaggcgg agcuguucua ccggaagcag uccaucccca     2640

agaagaucac ccaccccgcc aaggaggcca ucgccaacaa gaacaaggac aaccccaaga     2700

aggagagcgu cuucgaguac gaccucauca aggacaagcg cuucaccgag gacaaguucu     2760

ucuuccacug cccgaucacg aucaacuuca aguccagcgg ggccaacaag uucaacgacg     2820

agaucaaccu gcuccugaag gagaaggcga acgacgugca cauccugagc aucgaccggg     2880

gcgagcgcca ccucgccuac uacacccugg uggacgggaa gggcaacauc aucaagcagg     2940

acaccuucaa caucaucggg aacgaccgca ugaagaccaa cuaccacgac aagcuggccg     3000

ccaucgagaa ggaccgggac uccgcccgca aggacuggaa gaagaucaac aacaucaagg     3060

agaugaagga gggcuaccuc agccaggucg ugcacgagau cgcgaagcug gugaucgagu     3120

acaacgccau cgucguguuc gaggaccuga acuucggcuu caagcggggg cgcuucaagg     3180

uggagaagca ggucuaccag aagcucgaga agaugcugau cgagaagcug aacuaccucg     3240

uguucaagga caacgaguuc gacaagacgg gcggggugcu gcgggccuac cagcugaccg     3300

cccccuucga gaccuucaag aagaugggca agcagaccgg gaucaucuac uacguccccg     3360

cgggcuucac cuccaagauc ugccccguga cgggcuucgu gaaccagcuc uacccgaagu     3420

acgagagcgu cuccaagagc caggaguucu ucuccaaguu cgacaagauc ugcuacaacc     3480

uggacaaggg guacuucgag uucagcuucg acuacaagaa cuucggcgac aaggccgcca     3540

aggggaagug gaccaucgcc uccuucggca gccgccugau caacuuccgg aacuccgaca     3600

agaaccacaa cugggacacc cgcgaggugu accccaccaa ggagcucgag aagcugcuga     3660

aggacuacag caucgaguac ggccacgggg agugcaucaa ggccgcgauc ugcggcgagu     3720

ccgacaagaa guucuucgcc aagcucacga gcgugcugaa caccauccug cagaugcgga     3780

acuccaagac cgggaccgag cucgacuacc ugaucagccc cgucgccgac gugaacggca     3840

acuucuucga cucccgccag gcccccaaga acaugccgca ggacgccgac gcgaacgggg     3900

ccuaccacau cggccugaag ggccucaugc ugcuggggcg gaucaagaac aaccaggagg     3960

gcaagaagcu caaccuggug aucaagaacg aggaguacuu cgaguucguc cagaaccgca     4020

acaacaagcg gcccgccgcc acgaagaagg ccgggcaggc gaagaagaag aaguaaggac     4080

uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu     4140

cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa agaauu                                                     4216


<210>  2410
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2410
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgga caagaaguac      120

agcaucggcc uggacaucgg gaccaacucc gugggcuggg ccgugaucac cgacgaguac      180

aaggucccca gcaagaaguu caaggugcug gggaacacgg accggcacuc caucaagaag      240

aaccugaucg gcgcccugcu guucgacagc ggcgagaccg ccgaggccac ccgccugaag      300

cggaccgcgc gccggcgcua cacgcggcgc aagaaccgga ucugcuaccu gcaggagauc      360

uucuccaacg agauggccaa gguggacgac agcuucuucc accgccucga ggaguccuuc      420

cuggucgagg aggacaagaa gcacgagcgg caccccaucu ucgggaacau cguggacgag      480

guggccuacc acgagaagua ccccaccauc uaccaccucc gcaagaagcu cgucgacagc      540

accgacaagg ccgaccugcg gcugaucuac cucgcgcugg cccacaugau caaguuccgc      600

ggccacuucc ugaucgaggg ggaccucaac ccggacaacu ccgacgugga caagcuguuc      660

auccagcugg ugcagaccua caaccagcug uucgaggaga accccaucaa cgccagcggc      720

gucgacgcca aggccauccu guccgcgcgg cugagcaagu cccgccggcu cgagaaccug      780

aucgcccagc ugcccgggga gaagaagaac ggccucuucg gcaaccugau cgcccugagc      840

cucgggcuga cgcccaacuu caaguccaac uucgaccugg ccgaggacgc caagcuccag      900

cugagcaagg acaccuacga cgacgaccug gacaaccucc uggcgcagau cggcgaccag      960

uacgccgacc uguuccucgc cgccaagaac cuguccgacg ccauccugcu cagcgacauc     1020

cugcgcguga acaccgagau caccaaggcg ccgcuguccg ccagcaugau caagcgguac     1080

gacgagcacc accaggaccu cacgcugcug aaggcccucg ugcgccagca gcugcccgag     1140

aaguacaagg agaucuucuu cgaccagagc aagaacgggu acgccggcua caucgacggc     1200

ggggccuccc aggaggaguu cuacaaguuc aucaagccca uccuggagaa gauggacggc     1260

accgaggagc uccuggucaa gcugaaccgg gaggaccugc uccgcaagca gcgcaccuuc     1320

gacaacggga gcauccccca ccagauccac cugggcgagc ugcacgcgau ccuccggcgc     1380

caggaggacu ucuacccguu ccugaaggac aaccgggaga agaucgagaa gauccugacc     1440

uuccgcaucc ccuacuacgu ggggccccuc gcccggggca acucccgcuu cgccuggaug     1500

acgcgcaaga gcgaggagac caucaccccc uggaacuucg aggagguggu cgacaagggc     1560

gccuccgcgc agagcuucau cgagcggaug accaacuucg acaagaaccu gccgaacgag     1620

aaggugcugc ccaagcacuc ccuccuguac gaguacuuca cgguguacaa cgagcugacc     1680

aaggucaagu acgugaccga ggggaugcgg aagcccgccu uccucagcgg cgagcagaag     1740

aaggccaucg uggaccugcu guucaagacc aaccgcaagg ucacggugaa gcagcucaag     1800

gaggacuacu ucaagaagau cgagugcuuc gacuccgucg agaucagcgg gguggaggac     1860

cgguucaacg ccucccuggg caccuaccac gaccugcuca agaucaucaa ggacaaggac     1920

uuccuggaca acgaggagaa cgaggacauc cuggaggaca ucgugcucac ccugacccug     1980

uucgaggacc gcgagaugau cgaggagcgg cucaagacgu acgcccaccu guucgacgac     2040

aaggucauga agcagcugaa gcgccggcgc uacaccgggu ggggccggcu cagccgcaag     2100

cugaucaacg gcauccggga caagcagucc gggaagacca uccuggacuu ccucaagagc     2160

gacggcuucg cgaaccgcaa cuucaugcag cugauccacg acgacucccu gaccuucaag     2220

gaggacaucc agaaggccca ggugagcggg cagggcgacu cccuccacga gcacaucgcc     2280

aaccuggccg ggagccccgc caucaagaag ggcauccugc agacggugaa ggucguggac     2340

gagcucguga aggucauggg ccgccacaag ccggagaaca ucgugaucga gauggcgcgg     2400

gagaaccaga ccacccagaa ggggcagaag aacucccgcg agcggaugaa gcgcaucgag     2460

gagggcauca aggagcuggg gagccagauc cugaaggagc accccgugga gaacacccag     2520

cuccagaacg agaagcugua ccuguacuac cuccagaacg gccgggacau guacgucgac     2580

caggagcugg acaucaaccg ccugagcgac uacgacgugg accacaucgu gccccagucc     2640

uuccucaagg acgacagcau cgacaacaag guccugacgc gguccgacaa gaaccgcggc     2700

aagagcgaca acgugcccuc cgaggaggug gucaagaaga ugaagaacua cuggcggcag     2760

cugcucaacg ccaagcugau cacccagcgc aaguucgaca accugaccaa ggccgagcgc     2820

gggggccuca gcgagcugga caaggccggg uucaucaagc ggcagcuggu ggagacccgg     2880

cagaucacca agcacguggc ccagauccuc gacucccgca ugaacacgaa guacgacgag     2940

aacgacaagc ugauccggga ggucaaggug aucacccuca agagcaagcu cguguccgac     3000

uuccgcaagg acuuccaguu cuacaagguc cgggagauca acaacuacca ccacgcgcac     3060

gacgccuacc ugaacgccgu ggugggcacc gcccugauca agaaguaccc gaagcucgag     3120

agcgaguucg ucuacgggga cuacaaggug uacgacgugc gcaagaugau cgcgaagucc     3180

gagcaggaga ucggcaaggc caccgccaag uacuucuucu acagcaacau caugaacuuc     3240

uucaagacgg agaucacccu ggccaacggc gagauccgga agcgcccccu gaucgagacc     3300

aacggggaga ccggcgagau cgucugggac aaggggcggg acuucgccac ggugcgcaag     3360

gugcucucca ugccccaggu caacaucgug aagaagaccg aggugcagac cggcggguuc     3420

agcaaggagu ccauccugcc caagcgcaac agcgacaagc ugaucgcgcg gaagaaggac     3480

ugggacccga agaaguacgg cggcuucgac ucccccaccg ucgccuacag cgugcucgug     3540

gucgccaagg uggagaaggg caagagcaag aagcugaagu ccgugaagga gcugcucggg     3600

aucacgauca uggagcgcag cuccuucgag aagaacccca ucgacuuccu ggaggccaag     3660

ggguacaagg aggucaagaa ggaccugauc aucaagcucc cgaaguacag ccuguucgag     3720

cuggagaacg gccggaagcg caugcucgcc uccgcggggg agcugcagaa gggcaacgag     3780

cuggcccucc ccagcaagua cgugaacuuc cuguaccugg ccucccacua cgagaagcuc     3840

aagggcagcc ccgaggacaa cgagcagaag cagcuguucg uggagcagca caagcacuac     3900

cuggacgaga ucaucgagca gaucuccgag uucagcaagc gggucauccu cgccgacgcc     3960

aaccuggaca aggugcuguc cgcguacaac aagcaccgcg acaagcccau ccgggagcag     4020

gccgagaaca ucauccaccu cuucacccug accaaccugg gggccccggc cgcguucaag     4080

uacuucgaca ccacgaucga ccgcaagcgg uacaccagca ccaaggaggu gcucgacgcc     4140

acccugaucc accaguccau cacgggccug uacgagaccc gcaucgaccu cagccagcuc     4200

gggggcgaca agcggcccgc cgccaccaag aaggccgggc aggcgaagaa gaagaaguaa     4260

ggacuagugc aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau     4320

gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu     4380

cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa     4440

aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4530


<210>  2411
<211>  5313
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2411
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgaa cuucaagauc      120

cugcccaucg ccaucgaccu cggcgugaag aacaccgggg uguucagcgc cuucuaccag      180

aagggcaccu cccuggagcg gcuggacaac aagaacggga aggucuacga gcucagcaag      240

gacuccuaca cgcugcugau gaacaaccgc accgcccggc gccaccagcg gcgcggcauc      300

gaccggaagc agcucgugaa gcgccuguuc aagcugaucu ggaccgagca gcucaaccug      360

gagugggaca aggacaccca gcaggccauc agcuuccugu ucaaccggcg cggcuucucc      420

uucaucacgg acggguacag ccccgaguac cucaacaucg ugcccgagca ggucaaggcg      480

auccugaugg acaucuucga cgacuacaac ggcgaggacg accuggacuc cuaccucaag      540

cuggccaccg agcaggagag caagaucucc gagaucuaca acaagcugau gcagaagauc      600

cucgaguuca agcugaugaa gcugugcacc gacaucaagg acgacaaggu gagcaccaag      660

acgcucaagg agaucaccuc cuacgaguuc gagcugcugg ccgacuaccu cgccaacuac      720

agcgaguccc ugaagaccca gaaguucagc uacaccgaca agcaggggaa ccugaaggag      780

cucuccuacu accaccacga caaguacaac auccaggagu uccugaagcg gcacgcgacg      840

aucaacgacc gcauccugga cacccuccug accgacgacc uggacaucug gaacuucaac      900

uucgagaagu ucgacuucga caagaacgag gagaagcucc agaaccagga ggacaaggac      960

cacauccagg cccaccucca ccacuucgug uucgccguca acaagaucaa gagcgagaug     1020

gccuccggcg ggcggcaccg cagccaguac uuccaggaga ucaccaacgu gcuggacgag     1080

aacaaccacc aggagggcua ccugaagaac uucugcgaga accugcacaa caagaaguac     1140

uccaaccuga gcgugaagaa ccucgucaac cugaucggca accugagcaa ccucgagcug     1200

aagccgcugc ggaaguacuu caacgacaag auccacgcca aggcggacca cugggacgag     1260

cagaaguuca cggagaccua cugccacugg auccucgggg aguggcgcgu gggcgugaag     1320

gaccaggaca agaaggacgg ggccaaguac uccuacaagg accugugcaa cgagcugaag     1380

cagaagguca ccaaggccgg ccucguggac uuccugcugg agcuggaccc cugccggacc     1440

aucccgcccu accucgacaa caacaaccgc aagccgccca agugccagag ccugauccug     1500

aaccccaagu uccucgacaa ccaguacccc aacuggcagc aguaccugca ggagcugaag     1560

aagcuccagu ccauccagaa cuaccuggac agcuucgaga cggaccugaa ggugcucaag     1620

uccagcaagg accagcccua cuucgucgag uacaagucca gcaaccagca gaucgccucc     1680

ggccagcggg acuacaagga ccuggacgcc cgcauccugc aguucaucuu cgaccgggug     1740

aaggcgagcg acgagcuccu gcugaacgag aucuacuucc aggccaagaa gcucaagcag     1800

aaggccucca gcgagcugga gaagcuggag uccagcaaga agcucgacga ggugaucgcc     1860

aacucccagc ugagccagau ccugaagucc cagcacacca acgggaucuu cgagcagggc     1920

accuuccucc accuggucug caaguacuac aagcagcgcc agcgcgcccg ggacagccgc     1980

cuguacauca ugcccgagua ccgguacgac aagaagcucc acaaguacaa caacaccggg     2040

cgcuucgacg acgacaacca gcugcugacg uacugcaacc acaagccccg gcagaagcgc     2100

uaccagcucc ugaacgaccu ggcgggcgug cuccaggugu cccccaacuu ccugaaggac     2160

aagaucggga gcgacgacga ccuguucauc agcaaguggc ucgucgagca cauccggggc     2220

uucaagaagg ccugcgagga cucccugaag auccagaagg acaaccgcgg ccugcucaac     2280

cacaagauca acaucgcccg gaacaccaag gggaagugcg agaaggagau cuucaaccug     2340

aucugcaaga ucgagggcag cgaggacaag aaggggaacu acaagcacgg ccuggccuac     2400

gagcucgggg ugcugcuguu cggcgagccg aacgaggccu ccaagcccga guucgaccgc     2460

aagaucaaga aguucaacag caucuacucc uucgcgcaga uccagcagau cgccuucgcc     2520

gagcggaagg gcaacgccaa cacgugcgcg gucugcagcg ccgacaacgc ccaccgcaug     2580

cagcagauca agaucaccga gcccguggag gacaacaagg acaagaucau ccucuccgcc     2640

aaggcccagc ggcugcccgc gaucccgacc cgcaucgugg acggggccgu caagaagaug     2700

gccaccaucc uggccaagaa caucguggac gacaacuggc agaacaucaa gcagguccuc     2760

agcgccaagc accagcugca cauccccauc aucaccgagu ccaacgcguu cgaguucgag     2820

cccgcccugg ccgacgugaa gggcaagagc cucaaggacc ggcgcaagaa ggcccuggag     2880

cggaucuccc ccgagaacau cuucaaggac aagaacaacc gcaucaagga guucgccaag     2940

gggaucagcg cguacuccgg cgccaaccug accgacgggg acuucgacgg cgccaaggag     3000

gagcucgacc acaucauccc gcggagccac aagaaguacg gcacgcugaa cgacgaggcc     3060

aaccugaucu gcgugacccg cggggacaac aagaacaagg gcaaccgcau cuucugccuc     3120

cgggaccugg cggacaacua caagcugaag caguucgaga ccaccgacga ccucgagauc     3180

gagaagaaga ucgccgacac gaucugggac gccaacaaga aggacuucaa guucgggaac     3240

uaccgcuccu ucaucaaccu gacgccccag gagcagaagg ccuuccggca cgcccuguuc     3300

cucgcggacg agaaccccau caagcaggcc gugauccgcg ccaucaacaa ccggaaccgc     3360

accuucguca acggcaccca gcgguacuuc gccgaggugc uggccaacaa caucuaccug     3420

cgcgcgaaga aggagaaccu caacaccgac aagaucagcu ucgacuacuu cggcaucccc     3480

accaucggga acggccgggg gaucgccgag auccgccagc uguacgagaa gguggacucc     3540

gacauccagg ccuacgccaa gggcgacaag ccgcaggcca gcuacagcca ccugaucgac     3600

gcgaugcucg ccuucugcau cgccgccgac gagcaccgga acgacggguc caucggccug     3660

gagaucgaca agaacuacag ccuguacccc cucgacaaga acaccggcga ggucuucacc     3720

aaggacaucu ucucccagau caagaucacc gacaacgagu ucagcgacaa gaagcuggug     3780

cgcaagaagg cgaucgaggg guucaacacg caccggcaga ugacccgcga cggcaucuac     3840

gccgagaacu accugcccau ccucauccac aaggagcuga acgaggugcg gaagggguac     3900

accuggaaga acuccgagga gaucaagauc uucaagggca agaaguacga cauccagcag     3960

cugaacaacc ucgucuacug ccugaaguuc guggacaagc cgaucagcau cgacauccag     4020

aucuccaccc uggaggagcu ccgcaacauc cugacgacca acaacaucgc cgccaccgcc     4080

gaguacuacu acaucaaccu gaagacccag aagcuccacg aguacuacau cgagaacuac     4140

aacacggcgc ugggguacaa gaaguacagc aaggagaugg aguuccugcg gucccucgcc     4200

uaccgcagcg agcgggugaa gaucaagucc aucgacgacg ucaagcaggu ccuggacaag     4260

gacagcaacu ucaucaucgg caagaucacc cugcccuuca agaaggagug gcagcgccuc     4320

uaccgggagu ggcagaacac caccaucaag gacgacuacg aguuccugaa guccuucuuc     4380

aacgucaaga gcaucacgaa gcugcacaag aaggugcgca aggacuucuc ccuccccauc     4440

agcaccaacg agggcaaguu ccuggugaag cgcaagaccu gggacaacaa cuucaucuac     4500

cagauccuga acgacuccga cagccgggcc gacgggacca agcccuucau cccggccuuc     4560

gacaucucca agaacgagau cguggaggcc aucaucgaca gcuucacgag caagaacauc     4620

uucuggcucc ccaagaacau cgagcugcag aagguggaca acaagaacau cuucgcgauc     4680

gacaccucca agugguucga gguggagacc cccagcgacc ugcgcgacau cggcaucgcc     4740

accauccagu acaagaucga caacaacucc cggcccaagg ugcgcgucaa gcuggacuac     4800

gugaucgacg acgacagcaa gaucaacuac uucaugaacc acucccuccu caagagccgg     4860

uacccggaca aggugcugga gauccugaag caguccacga ucaucgaguu cgagagcucc     4920

ggguucaaca agaccaucaa ggagaugcug ggcaugaagc uggccgggau cuacaacgag     4980

accagcaaca acaagcgccc cgccgccacc aagaaggcgg gccaggccaa gaagaagaag     5040

uaaggacuag ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa     5100

aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc     5160

ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau     5220

aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5280

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  5313


<210>  2412
<211>  3585
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2412
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgaa gcggaacuac      120

auccugggcc ucgacaucgg gaucaccagc gugggcuacg ggaucaucga cuacgagacc      180

cgcgacguga ucgacgccgg cguccggcug uucaaggagg ccaacgugga gaacaacgag      240

ggccgccggu ccaagcgcgg ggcccggcgc cugaagcggc gccggcgcca ccggauccag      300

cgcgugaaga agcuccuguu cgacuacaac cugcucacgg accacagcga gcuguccggc      360

aucaaccccu acgaggcccg ggucaagggg cugagccaga agcucuccga ggaggaguuc      420

agcgcggccc ugcugcaccu cgccaagcgc cggggcgugc acaacgugaa cgaggucgag      480

gaggacaccg ggaacgagcu guccaccaag gagcagauca gccgcaacuc caaggcccug      540

gaggagaagu acguggcgga gcuccagcug gagcggcuga agaaggacgg cgaggugcgc      600

ggcagcauca accgguucaa gaccuccgac uacgucaagg aggccaagca gcuccugaag      660

gugcagaagg ccuaccacca gcuggaccag agcuucaucg acacguacau cgaccuccug      720

gagacccgcc gcaccuacua cgaggggccc ggcgaggggu cccccuucgg cuggaaggac      780

aucaaggagu gguacgagau gcugaugggc cacugcaccu acuucccgga ggagcuccgg      840

agcgugaagu acgccuacaa cgccgaccug uacaacgcgc ugaacgaccu caacaaccug      900

gucaucacgc gcgacgagaa cgagaagcug gaguacuacg agaaguucca gaucaucgag      960

aacguguuca agcagaagaa gaagcccacc cucaagcaga ucgccaagga gauccuggug     1020

aacgaggagg acaucaaggg guaccggguc accuccaccg gcaagcccga guucacgaac     1080

cugaaggugu accacgacau caaggacauc accgcccgca aggagaucau cgagaacgcc     1140

gagcuccugg accagaucgc caagauccug accaucuacc agagcuccga ggacauccag     1200

gaggagcuca ccaaccugaa cagcgagcug acgcaggagg agaucgagca gaucuccaac     1260

cucaaggggu acaccggcac ccacaaccug agccugaagg cgaucaaccu cauccuggac     1320

gagcuguggc acaccaacga caaccagauc gccaucuuca accggcucaa gcuggugccc     1380

aagaaggucg accugagcca gcagaaggag aucccgacga cccucgugga cgacuucauc     1440

cugucccccg uggucaagcg cagcuucauc caguccauca aggugaucaa cgccaucauc     1500

aagaaguacg ggcugcccaa cgacaucauc aucgagcugg cccgggagaa gaacagcaag     1560

gacgcccaga agaugaucaa cgagaugcag aagcgcaacc ggcagaccaa cgagcgcauc     1620

gaggagauca uccggaccac gggcaaggag aacgcgaagu accucaucga gaagaucaag     1680

cugcacgaca ugcaggaggg caagugccug uacucccucg aggccauccc ccuggaggac     1740

cugcucaaca acccguucaa cuacgagguc gaccacauca ucccccgcag cguguccuuc     1800

gacaacagcu ucaacaacaa ggugcugguc aagcaggagg agaacuccaa gaaggggaac     1860

cggacccccu uccaguaccu gagcuccagc gacuccaaga ucagcuacga gaccuucaag     1920

aagcacaucc ucaaccuggc caagggcaag gggcgcaucu ccaagaccaa gaaggaguac     1980

cugcucgagg agcgggacau caaccgcuuc agcgugcaga aggacuucau caaccggaac     2040

cugguggaca cgcgcuacgc cacccggggc cugaugaacc uccugcgcuc cuacuuccgc     2100

gucaacaacc uggacgugaa ggucaagagc aucaacgggg gcuucaccuc cuuccuccgg     2160

cgcaagugga aguucaagaa ggagcggaac aagggcuaca agcaccacgc cgaggacgcg     2220

cugaucaucg ccaacgccga cuucaucuuc aaggagugga agaagcugga caaggccaag     2280

aaggugaugg agaaccagau guucgaggag aagcaggcgg agagcaugcc cgagaucgag     2340

accgagcagg aguacaagga gaucuucauc acgccgcacc agaucaagca caucaaggac     2400

uucaaggacu acaaguacag ccaccgcgug gacaagaagc ccaaccggga gcucaucaac     2460

gacacccugu acuccacccg caaggacgac aaggggaaca cccugaucgu gaacaaccuc     2520

aacggccugu acgacaagga caacgacaag cugaagaagc ucaucaacaa gagccccgag     2580

aagcugcuga uguaccacca cgacccccag acguaccaga agcucaagcu gaucauggag     2640

caguacgggg acgagaagaa cccgcuguac aaguacuacg aggagaccgg caacuaccuc     2700

accaaguacu ccaagaagga caacgggccc gucaucaaga agaucaagua cuacggcaac     2760

aagcugaacg cccaccugga caucaccgac gacuacccca acagccggaa caagguggug     2820

aagcucuccc ugaagcccua ccgcuucgac gucuaccugg acaacggcgu guacaaguuc     2880

gugaccguca agaaccucga cgugaucaag aaggagaacu acuacgaggu gaacagcaag     2940

ugcuacgagg aggccaagaa gcugaagaag aucuccaacc aggccgaguu caucgccagc     3000

uucuacaaca acgaccugau caagaucaac ggggagcucu accgggucau cggcgugaac     3060

aacgaccugc ugaaccgcau cgaggugaac augaucgaca ucacguaccg ggaguaccuc     3120

gagaacauga acgacaagcg cccgccccgg aucaucaaga ccaucgcguc caagacccag     3180

agcaucaaga aguacuccac cgacauccug gggaaccugu acgaggucaa gagcaagaag     3240

cacccccaga ucaucaagaa gggcaagcgc cccgccgcca cgaagaaggc cggccaggcc     3300

aagaagaaga aguaaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa     3360

uaagagaaag aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua     3420

aagccaacac ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu     3480

gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3585


<210>  2413
<211>  4653
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2413
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgcu guucaacaag      120

ugcaucauca ucagcaucaa ccucgacuuc uccaacaagg agaagugcau gaccaagccc      180

uacagcaucg gccuggacau cgggaccaac uccgugggcu gggccgugau cacggacaac      240

uacaaggucc ccagcaagaa gaugaaggug cuggggaaca ccuccaagaa guacaucaag      300

aagaaccucc ugggcgugcu gcuguucgac agcggcauca ccgccgaggg gcggcgccug      360

aagcggaccg cccgccggcg cuacacgcgg cgccggaacc gcauccugua ccuccaggag      420

aucuucucca ccgagauggc cacccuggac gacgcguucu uccagcggcu ggacgacagc      480

uuccucgucc ccgacgacaa gcgcgacucc aaguacccga ucuucggcaa ccugguggag      540

gagaaggugu accacgacga guuccccacc aucuaccacc uccggaagua ccucgccgac      600

agcacgaaga aggccgaccu gcgccugguc uaccucgccc uggcgcacau gaucaaguac      660

cgggggcacu uccugaucga gggcgaguuc aacuccaaga acaacgacau ccagaagaac      720

uuccaggacu uccucgacac cuacaacgcc aucuucgaga gcgaccuguc ccuggagaac      780

agcaagcagc ucgaggagau cgugaaggac aagaucucca agcuggagaa gaaggaccgc      840

auccugaagc uguuccccgg ggagaagaac agcggcaucu ucuccgaguu ccugaagcug      900

aucgugggca accaggccga cuuccggaag ugcuucaacc ucgacgagaa ggccagccug      960

cacuucucca aggagagcua cgacgaggac cuggagaccc uccuggggua caucggcgac     1020

gacuacagcg acgucuuccu gaaggccaag aagcucuacg acgcgauccu gcuguccggg     1080

uuccucaccg ugacggacaa cgagaccgag gcgccccuga gcuccgccau gaucaagcgc     1140

uacaacgagc acaaggagga ccuggcccuc cugaaggagu acauccggaa caucagccug     1200

aagaccuaca acgagguguu caaggacgac accaagaacg gcuacgccgg cuacaucgac     1260

gggaagacga accaggagga cuucuacguc uaccucaaga accugcuggc cgaguucgag     1320

ggcgccgacu acuuccucga gaagaucgac cgcgaggacu uccugcggaa gcagcgcacc     1380

uucgacaacg gguccauccc guaccagauc caccuccagg agaugcgcgc cauccuggac     1440

aagcaggcca aguucuaccc cuuccucgcc aagaacaagg agcggaucga gaagauccug     1500

accuuccgca uccccuacua cgugggcccc cuggcgcggg ggaacagcga cuucgccugg     1560

uccauccgca agcggaacga gaagaucacc ccguggaacu ucgaggacgu gaucgacaag     1620

gagagcuccg ccgaggccuu caucaaccgc augacgagcu ucgaccucua ccugcccgag     1680

gagaaggucc ugcccaagca cucccuccug uacgagaccu ucaacgugua caacgagcug     1740

accaaggugc gguucaucgc cgagagcaug cgcgacuacc aguuccucga cuccaagcag     1800

aagaaggaca ucguccggcu guacuucaag gacaagcgca aggugaccga caaggacauc     1860

aucgaguacc ugcacgcgau cuacggcuac gacggcaucg agcucaaggg gaucgagaag     1920

caguucaaca gcucccugag cacguaccac gaccugcuca acaucaucaa cgacaaggag     1980

uuccuggacg acuccagcaa cgaggccauc aucgaggaga ucauccacac ccugaccauc     2040

uucgaggacc gggagaugau caagcagcgc cucuccaagu ucgagaacau cuucgacaag     2100

agcgugcuga agaagcugag ccggcgccac uacaccggcu gggggaagcu cuccgccaag     2160

cugaucaacg gcauccggga cgagaagucc gggaacacga uccuggacua ccucaucgac     2220

gacggcauca gcaaccgcaa cuucaugcag cugauccacg acgacgcccu gagcuucaag     2280

aagaagaucc agaaggcgca gaucaucggc gacgaggaca aggggaacau caaggagguc     2340

gugaaguccc uccccggcag cccggccauc aagaagggga uccugcaguc caucaagauc     2400

gucgacgagc uggugaaggu gaugggcggg cggaagcccg agagcaucgu cguggagaug     2460

gcccgcgaga accaguacac caaccagggc aaguccaaca gccagcagcg gcucaagcgc     2520

cuggagaagu cccugaagga gcucggcagc aagauccuga aggagaacau ccccgccaag     2580

cuguccaaga ucgacaacaa cgcccuccag aacgaccgcc uguaccugua cuaccuccag     2640

aacgggaagg acauguacac cggcgacgac cuggacaucg accggcugag caacuacgac     2700

aucgaccaca ucauccccca ggcguuccuc aaggacaacu ccaucgacaa caaggugcug     2760

gucagcuccg ccagcaaccg cgggaagucc gacgacuucc cgagccugga gguggugaag     2820

aagcggaaga ccuucuggua ccagcuccuc aagagcaagc ugaucuccca gcgcaaguuc     2880

gacaaccuga cgaaggccga gcggggcggc cugcugcccg aggacaaggc cggguucauc     2940

cagcgccagc ucgucgagac ccggcagauc accaagcacg uggcccgccu gcuggacgag     3000

aaguucaaca acaagaagga cgagaacaac cgggcggugc gcaccgucaa gaucaucacg     3060

cucaagagca cccugguguc ccaguuccgg aaggacuucg agcuguacaa ggugcgcgag     3120

aucaacgacu uccaccacgc ccacgacgcc uaccucaacg ccgucaucgc cagcgcgcug     3180

cugaagaagu accccaagcu cgagcccgag uucguguacg gcgacuaccc gaaguacaac     3240

uccuuccggg agcgcaagag cgccaccgag aagguguacu ucuacuccaa caucaugaac     3300

aucuucaaga aguccaucag ccuggccgac gggcggguca ucgagcgccc ccugaucgag     3360

gugaacgagg agaccggcga gagcgugugg aacaaggagu ccgaccucgc caccguccgg     3420

cgcgugcuga gcuaccccca ggugaacguc gugaagaagg uggaggagca gaaccacggg     3480

cuggaccggg gcaagcccaa gggccucuuc aacgcgaacc uguccagcaa gccgaagccc     3540

aacuccaacg agaaccuggu cggggccaag gaguaccucg accccaagaa guacggcggg     3600

uacgccggca ucagcaacuc cuucgccgug cuggugaagg ggacgaucga gaagggcgcc     3660

aagaagaaga ucaccaacgu ccuggaguuc cagggcauca gcauccucga ccgcaucaac     3720

uaccggaagg acaagcugaa cuuccugcuc gagaaggggu acaaggacau cgagcugauc     3780

aucgagcugc cgaaguacag ccucuucgag cuguccgacg gcagccgccg caugcuggcg     3840

uccauccuca gcaccaacaa caagcgcggg gagauccaca agggcaacca gaucuuccug     3900

ucccagaagu ucgugaagcu gcucuaccac gccaagcgga ucagcaacac caucaacgag     3960

aaccaccgga aguacgugga gaaccacaag aaggaguucg aggagcuguu cuacuacauc     4020

cuggaguuca acgagaacua cgucggggcc aagaagaacg gcaagcuccu gaacuccgcc     4080

uuccagagcu ggcagaacca cuccaucgac gagcugugca gcuccuucau cggccccacg     4140

gggagcgagc gcaagggccu cuucgagcug accucccggg ggagcgccgc ggacuucgag     4200

uuccugggcg ugaagauccc ccgcuaccgg gacuacaccc ccuccagccu ccugaaggac     4260

gccacccuga uccaccaguc cgugacgggg cuguacgaga cccgcaucga ccucgccaag     4320

cugggcgagg gcaagcggcc ggccgccacc aagaaggcgg ggcaggccaa gaagaagaag     4380

uaaggacuag ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa     4440

aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc     4500

ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau     4560

aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  4653


<210>  2414
<211>  3729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2414
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgug guacgccagc      120

cugauguccg cccaccaccu ccgggugggc aucgacgugg ggacccacag cgucggccug      180

gccacccugc gcguggacga ccacgggacg cccaucgagc uccuguccgc ccugagccac      240

auccacgacu ccggcguggg caaggagggg aagaaggacc acgacacccg gaagaagcuc      300

agcggcaucg cgcgccgggc ccgccggcug cugcaccacc gccggaccca gcuccagcag      360

cuggacgagg uccugcgcga ccucggguuc cccaucccca ccccgggcga guuccuggac      420

cugaacgagc agacggaccc cuaccgggug uggcgcgugc gggcccgccu cgucgaggag      480

aagcugcccg aggagcugcg ggggcccgcc aucuccaugg cggugcgcca caucgcccgg      540

caccgcggcu ggcggaaccc guacagcaag guggaguccc uccugagccc cgccgaggag      600

ucccccuuca ugaaggcccu gcgcgagcgg auccucgcca ccaccggcga gguccuggac      660

gacgggauca cccccggcca ggcgauggcc cagguggccc ugacgcacaa caucagcaug      720

cgcgggccgg agggcauccu cggcaagcug caccaguccg acaacgccaa cgagauccgc      780

aagaucugcg cccggcaggg ggugagcccc gacgucugca agcagcugcu ccgcgcggug      840

uucaaggccg acagcccccg gggcuccgcc gugucccgcg ucgcccccga cccgcugccc      900

gggcagggca gcuuccggcg cgcccccaag ugcgaccccg aguuccagcg guuccgcauc      960

aucuccaucg uggcgaaccu gcggaucagc gagaccaagg gggagaaccg cccgcucacc     1020

gccgacgagc ggcgccacgu ggucaccuuc cugacggagg acagccaggc cgaccugacc     1080

uggguggacg uggccgagaa gcucggcguc caccggcgcg accugcgggg caccgccgug     1140

cacaccgacg acggggagcg cuccgcggcc cggccgccca ucgacgccac ggaccgcauc     1200

augcggcaga ccaagaucag cucccugaag accugguggg aggaggccga cagcgagcag     1260

cgcggcgcga ugauccgcua ccucuacgag gaccccaccg acuccgagug cgccgagauc     1320

aucgccgagc ugcccgagga ggaccaggcc aagcuggaca gccuccaccu ccccgccggg     1380

cgggcggccu acucccgcga gagccugacg gcccuguccg accacaugcu ggccaccacc     1440

gacgaccugc acgaggcccg gaagcgccuc uucggcgucg acgacagcug ggcgccgccc     1500

gccgaggcca ucaacgcccc cguggggaac cccuccgugg accggacccu gaagaucguc     1560

ggccgcuacc ugagcgccgu ggaguccaug uggggcacgc ccgaggugau ccacgucgag     1620

cacgugcggg acggguucac cagcgagcgc auggcggacg agcgggacaa ggccaaccgc     1680

cggcgcuaca acgacaacca ggaggccaug aagaagaucc agcgggacua cggcaaggag     1740

ggguacaucu cccgcggcga caucgugcgg cucgacgccc uggagcugca ggggugcgcg     1800

ugccuguacu gcggcaccac caucggcuac cacacgugcc agcucgacca caucgucccg     1860

caggccgggc ccggcagcaa caaccgccgg gggaaccugg uggccgugug cgagcgcugc     1920

aaccggucca agagcaacac ccccuucgcc gucugggccc agaagugcgg caucccccac     1980

gugggcguga aggaggcgau cgggcgcguc cggggcuggc gcaagcagac cccgaacacc     2040

uccagcgagg accugacgcg ccucaagaag gaggugaucg cccggcugcg ccggacccag     2100

gaggaccccg agaucgacga gcgcagcaug gaguccgugg ccuggauggc caacgagcug     2160

caccaccgga ucgccgcggc cuaccccgag accaccguca ugguguaccg cgggagcauc     2220

acggccgccg cccggaaggc ggccggcauc gacucccgca ucaaccucau cggggagaag     2280

ggccggaagg accgcaucga ccggcgccac cacgccgugg acgccagcgu cguggcgcug     2340

auggaggccu ccguggccaa gacccuggcc gagcggagcu cccuccgcgg cgagcagcgg     2400

cugaccggga aggagcagac cuggaagcag uacaccggca gcacggucgg ggcccgcgag     2460

cacuucgaga uguggcgggg ccacaugcug caccucaccg agcuguucaa cgagcgccug     2520

gcggaggaca agguguacgu gacccagaac auccggcucc gccuguccga cgggaacgcc     2580

cacaccguca accccagcaa gcuggugucc caccgccucg gcgacggccu gacggugcag     2640

cagaucgacc gggccugcac cccggcccug uggugcgccc ucacccgcga gaaggacuuc     2700

gacgagaaga acgggcugcc cgcgcgggag gaccgcgcca uccgggucca cggccacgag     2760

aucaagagcu ccgacuacau ccagguguuc agcaagcgca agaagaccga cuccgaccgg     2820

gacgagacgc ccuucggggc caucgccgug cgcggcgggu ucgucgagau cggccccagc     2880

auccaccacg cccggaucua ccgcguggag ggcaagaagc cgguguacgc gaugcugcgg     2940

gucuucaccc acgaccuccu gucccagcgc cacggggacc uguucagcgc cgugaucccg     3000

ccccagagca ucuccaugcg gugcgccgag cccaagcucc gcaaggccau caccaccggc     3060

aacgccacgu accuggggug gguggucgug ggcgacgagc uggagaucaa cguggacagc     3120

uucaccaagu acgcgaucgg gcgguuccuc gaggacuucc ccaacaccac ccgcuggcgg     3180

aucugcggcu acgacacgaa cuccaagcug acccugaagc ccaucguccu cgccgccgag     3240

ggccuggaga accccagcuc cgccgugaac gagaucgugg agcugaaggg guggcgcguc     3300

gcgaucaacg ugcucaccaa ggugcacccc accgucgugc ggcgcgacgc ccugggccgg     3360

ccgcgcuaca gcucccgcag caaccugccc acguccugga ccaucgagaa gcggcccgcc     3420

gccaccaaga aggccgggca ggcgaagaag aagaaguaag gacuagugca ucacauuuaa     3480

aagcaucuca gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau     3540

cucuuuuucu uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu     3600

aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaagaauu                                                             3729


<210>  2415
<211>  4590
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2415
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgac caagcccuac      120

agcaucggcc uggacaucgg gaccaacucc gugggcuggg ccgugacgac cgacaacuac      180

aaggucccca gcaagaagau gaaggugcuc gggaacaccu ccaagaagua caucaagaag      240

aaccugcugg gcgugcuccu guucgacagc ggcaucaccg ccgaggggcg gcgccugaag      300

cggacggccc gccggcgcua cacccggcgc cggaaccgca uccucuaccu gcaggagauc      360

uucuccaccg agauggccac ccuggacgac gcguucuucc agcggcucga cgacagcuuc      420

cugguccccg acgacaagcg cgacuccaag uacccgaucu ucggcaaccu gguggaggag      480

aaggccuacc acgacgaguu ccccacgauc uaccaccucc ggaaguaccu ggccgacagc      540

accaagaagg ccgaccugcg ccucguguac cuggcgcugg cccacaugau caaguaccgg      600

gggcacuucc ucaucgaggg cgaguucaac uccaagaaca acgacaucca gaagaacuuc      660

caggacuucc uggacaccua caacgccauc uucgagagcg accugucccu cgagaacagc      720

aagcagcugg aggagaucgu caaggacaag aucuccaagc uggagaagaa ggaccgcauc      780

cucaagcugu uccccgggga gaagaacagc ggcaucuucu ccgaguuccu gaagcucauc      840

gugggcaacc aggccgacuu ccggaagugc uucaaccugg acgagaaggc cagccugcac      900

uucuccaagg agagcuacga cgaggaccuc gagacccugc ugggguacau cggcgacgac      960

uacuccgacg uguuccucaa ggcgaagaag cuguacgacg ccauccugcu cagcggguuc     1020

cugacgguca ccgacaacga gaccgaggcg ccccugagcu ccgccaugau caagcgcuac     1080

aacgagcaca aggaggaccu cgcccugcug aaggaguaca uccggaacau cagccucaag     1140

accuacaacg agguguucaa ggacgacacg aagaacggcu acgccggcua caucgacggg     1200

aagaccaacc aggaggacuu cuacguguac cugaagaagc ugcucgccga guucgagggc     1260

gccgacuacu uccuggagaa gaucgaccgc gaggacuucc ugcggaagca gcgcaccuuc     1320

gacaacgggu ccaucccgua ccagauccac cuccaggaga ugcgcgccau ccuggacaag     1380

caggccaagu ucuaccccuu ccuggcgaag aacaaggagc ggaucgagaa gauccugacc     1440

uuccgcaucc ccuacuacgu cggcccccuc gcccggggga acagcgacuu cgccuggucc     1500

auccgcaagc ggaacgagaa gaucacgccg uggaacuucg aggacgugau cgacaaggag     1560

agcuccgccg aggccuucau caaccgcaug accagcuucg accuguaccu gcccgaggag     1620

aaggugcucc ccaagcacuc ccugcuguac gagaccuuca acgucuacaa cgagcucacc     1680

aaggugcggu ucaucgcgga gagcaugcgc gacuaccagu uccuggacuc caagcagaag     1740

aaggacaucg ugcggcugua cuucaaggac aagcgcaagg ucacggacaa ggacaucauc     1800

gaguaccucc acgccaucua cggcuacgac ggcaucgagc ugaaggggau cgagaagcag     1860

uucaacagcu cccugagcac cuaccacgac cuccugaaca ucaucaacga caaggaguuc     1920

cuggacgacu ccagcaacga ggccaucauc gaggagauca uccacacccu caccaucuuc     1980

gaggaccggg agaugaucaa gcagcgccug uccaaguucg agaacaucuu cgacaagagc     2040

gugcugaaga agcucucccg gcgccacuac acgggcuggg ggaagcugag cgccaagcug     2100

aucaacggca uccgggacga gaaguccggg aacaccaucc ucgacuaccu gaucgacgac     2160

ggcaucagca accgcaacuu caugcagcug auccacgacg acgcgcucag cuucaagaag     2220

aagauccaga aggcccagau caucggcgac gaggacaagg ggaacaucaa ggaggugguc     2280

aagucccugc ccggcagccc ggccaucaag aaggggaucc ugcaguccau caagaucgug     2340

gacgagcucg ucaaggugau gggcgggcgg aagcccgaga gcaucguggu cgagauggcc     2400

cgcgagaacc aguacaccaa ccagggcaag uccaacagcc agcagcggcu gaagcgccug     2460

gagaaguccc ucaaggagcu gggcagcaag auccugaagg agaacauccc cgccaagcuc     2520

uccaagaucg acaacaacgc gcugcagaac gaccgccugu accucuacua ccugcagaac     2580

gggaaggaca uguacaccgg cgacgaccug gacaucgacc ggcucagcaa cuacgacauc     2640

gaccacauca ucccccaggc cuuccugaag gacaacucca ucgacaacaa ggugcuggug     2700

agcuccgcca gcaaccgcgg gaaguccgac gacgucccga gccucgaggu ggugaagaag     2760

cggaagacgu ucugguacca gcugcucaag agcaagcuga ucucccagcg caaguucgac     2820

aaccugacca aggccgagcg gggcggccug agccccgagg acaaggccgg guucauccag     2880

cgccagcucg ucgagacccg gcagaucacc aagcacgugg cgcgccugcu ggacgagaag     2940

uucaacaaca agaaggacga gaacaaccgg gccgugcgca cggucaagau caucacccuc     3000

aaguccaccc uggugagcca guuccggaag gacuucgagc uguacaaggu gcgcgagauc     3060

aacgacuucc accacgccca cgacgccuac cucaacgccg ucguggcguc cgcccugcug     3120

aagaaguacc ccaagcucga gcccgaguuc guguacggcg acuacccgaa guacaacagc     3180

uuccgggagc gcaaguccgc caccgagaag gucuacuucu acagcaacau caugaacauc     3240

uucaagaagu ccaucagccu ggccgacggg cgggugaucg agcgcccccu gaucgaggug     3300

aacgaggaga ccggcgaguc cgucuggaac aaggagagcg accucgcgac ggugcggcgc     3360

gugcuguccu acccccaggu caacguggug aagaaggucg aggagcagaa ccacgggcug     3420

gaccggggca agcccaaggg ccucuucaac gccaaccuga gcuccaagcc gaagcccaac     3480

agcaacgaga accugguggg ggccaaggag uaccucgacc ccaagaagua cggcggguac     3540

gccggcaucu ccaacagcuu caccgugcug gucaagggga ccaucgagaa gggcgccaag     3600

aagaagauca ccaacgugcu ggaguuccag ggcaucucca uccucgaccg caucaacuac     3660

cggaaggaca agcugaacuu ccugcucgag aagggguaca aggacaucga gcugaucauc     3720

gagcugccga aguacagccu cuucgagcug agcgacggcu cccgccgcau gcuggcgagc     3780

auccucucca cgaacaacaa gcgcggggag auccacaagg gcaaccagau cuuccugagc     3840

cagaaguucg ugaagcugcu cuaccacgcc aagcggaucu ccaacaccau caacgagaac     3900

caccggaagu acgucgagaa ccacaagaag gaguucgagg agcuguucua cuacauccug     3960

gaguucaacg agaacuacgu gggggccaag aagaacggca agcuccugaa cagcgccuuc     4020

caguccuggc agaaccacag caucgacgag cugugcucca gcuucaucgg ccccaccggg     4080

uccgagcgca agggccucuu cgagcugacc agccgggggu ccgccgcgga cuucgaguuc     4140

cugggcguga agaucccccg cuaccgggac uacacgccca gcucccuccu gaaggacgcc     4200

acccugaucc accagagcgu caccgggcuc uacgagaccc gcaucgaccu ggccaagcug     4260

ggcgagggca agcggccggc cgccacgaag aaggcggggc aggccaagaa gaagaaguaa     4320

ggacuagugc aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau     4380

gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu     4440

cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa     4500

aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4590


<210>  2416
<211>  3672
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2416
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcggc cgccuucaag      120

cccaacagca ucaacuacau ccugggccuc gacaucggga ucgccuccgu gggcugggcc      180

augguggaga ucgacgagga ggagaacccc auccggcuga ucgaccuggg gguccgcgug      240

uucgagcggg cggaggugcc caagaccggc gacagccucg ccauggcccg ccggcuggcc      300

cgcuccgucc ggcgccugac ccggcgccgg gcgcaccgcc uccugcggac gcgccggcug      360

cucaagcgcg agggcgugcu gcaggccgcc aacuucgacg agaacgggcu gaucaagagc      420

cucccgaaca cccccuggca gcugcgggcc gccgcgcugg accgcaagcu cacgccccug      480

gagugguccg ccgugcugcu ccaccugauc aagcaccggg gcuaccugag ccagcgcaag      540

aacgaggggg agaccgccga caaggagcuc ggcgcccugc ugaagggggu cgccggcaac      600

gcgcacgccc uccagaccgg cgacuuccgg acccccgccg agcuggcccu gaacaaguuc      660

gagaaggagu ccgggcacau ccgcaaccag cgcagcgacu acucccacac cuucagccgg      720

aaggaccucc aggccgagcu gauccuccug uucgagaagc agaaggaguu cggcaacccg      780

cacguguccg ggggccugaa ggagggcauc gagacccugc ucaugacgca gcgccccgcg      840

cugagcgggg acgccgugca gaagaugcug ggccacugca ccuucgagcc cgccgagccc      900

aaggccgcca agaacaccua caccgcggag cgguucaucu ggcucacgaa gcugaacaac      960

cugcgcaucc ucgagcaggg guccgagcgg ccgcugaccg acaccgagcg cgccacccug     1020

auggacgagc ccuaccgcaa gagcaagcuc acguacgccc aggcccggaa gcugcugggc     1080

cucgaggaca ccgcguucuu caaggggcug cgguacggca aggacaacgc cgaggccucc     1140

acccugaugg agaugaaggc cuaccacgcc aucagccgcg cgcucgagaa ggagggccug     1200

aaggacaaga aguccccgcu gaaccugagc cccgagcucc aggacgagau cgggaccgcc     1260

uucucccugu ucaagacgga cgaggacauc accggccggc ugaaggaccg cauccagccg     1320

gagauccucg aggcccugcu gaagcacauc agcuucgaca aguucgucca gaucagccuc     1380

aaggcccugc ggcgcaucgu gccccugaug gagcagggga agcgguacga cgaggccugc     1440

gcggagaucu acggcgacca cuacgggaag aagaacaccg aggagaagau cuaccucccg     1500

cccauccccg ccgacgagau ccgcaacccc gugguccugc gggcccuguc ccaggcccgc     1560

aaggugauca acggcguggu ccggcgcuac ggcagccccg cccgcaucca caucgagacc     1620

gcgcgggagg uggggaaguc cuucaaggac cgcaaggaga ucgagaagcg gcaggaggag     1680

aaccgcaagg accgggagaa ggccgccgcc aaguuccgcg aguacuuccc caacuucgug     1740

ggcgagccca agagcaagga cauccucaag cugcggcugu acgagcagca gcacgggaag     1800

ugccucuacu ccggcaagga gaucaaccug gggcgccuga acgagaaggg cuacgucgag     1860

aucgaccacg cgcuccccuu cagccggacg ugggacgacu ccuucaacaa caaggugcug     1920

gugcugggca gcgagaacca gaacaagggg aaccagaccc ccuacgagua cuucaacggc     1980

aaggacaacu cccgcgagug gcaggaguuc aaggcccggg ucgagaccag ccgcuucccc     2040

cgguccaaga agcagcgcau ccuccugcag aaguucgacg aggacggguu caaggagcgg     2100

aaccugaacg acacccgcua cgugaaccgg uuccucugcc aguucgucgc cgaccgcaug     2160

cggcugacgg gcaagggcaa gaagcgcgug uucgccagca acgggcagau caccaaccug     2220

cuccggggcu ucugggggcu gcgcaaggug cgcgccgaga acgaccggca ccacgcgcug     2280

gacgccgucg ugguggccug cuccaccguc gccaugcagc agaagaucac ccgcuucgug     2340

cgguacaagg agaugaacgc cuucgacggc aagacgaucg acaaggagac cggggaggug     2400

cuccaccaga agacccacuu cccgcagccc ugggaguucu ucgcgcagga ggucaugauc     2460

cgcguguucg gcaagcccga cggcaagccg gaguucgagg aggccgacac ccuggagaag     2520

cugcggacgc uccuggccga gaagcugagc ucccgccccg aggccgugca cgaguacguc     2580

acgccccucu ucgugagccg ggcccccaac cgcaagaugu ccgggcaggg ccacauggag     2640

accgucaaga gcgcgaagcg gcuggacgag gggguguccg ugcugcgcgu gccgcucacc     2700

cagcugaagc ugaaggaccu cgagaagaug gucaaccggg agcgcgagcc caagcuguac     2760

gaggcccuga aggcccggcu cgaggcccac aaggacgacc ccgcgaaggc cuucgccgag     2820

cccuucuaca aguacgacaa ggccggcaac cgcacccagc aggucaaggc cgugcgggug     2880

gagcaggucc agaagaccgg ggugugggug cgcaaccaca acggcaucgc ggacaacgcc     2940

accauggugc ggguggacgu cuucgagaag ggcgacaagu acuaccuggu gccgaucuac     3000

agcuggcagg ucgccaaggg gauccugccc gaccgcgccg uggugcaggg caaggacgag     3060

gaggacuggc agcucaucga cgacagcuuc aacuucaagu ucucccugca ccccaacgac     3120

cugguggagg ucaucaccaa gaaggcccgg auguucgggu acuucgcgag cugccaccgc     3180

ggcaccggga acaucaacau ccgcauccac gaccucgacc acaagaucgg caagaacggc     3240

auccuggagg ggaucggcgu gaagacggcc cuguccuucc agaaguacca gaucgacgag     3300

cucgggaagg agauccggcc cugccgccug aagaagcggc cgcccgugcg caagcggccc     3360

gccgccacca agaaggccgg ccaggcgaag aagaagaagu aaggacuagu gcaucacauu     3420

uaaaagcauc ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu     3480

caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc     3540

uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga     3600

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaagaa uu                                                         3672


<210>  2417
<211>  4428
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2417
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgaa gaagcccuac      120

accaucggcc uggacaucgg gaccaacagc gugggcuggg ccgugcucac ggaccaguac      180

gaccugguca agcggaagau gaagaucgcc ggggacuccg agaagaagca gaucaagaag      240

aacuucuggg gcgugcgccu guucgacgag ggccagaccg ccgccgaccg gcgcauggcg      300

cggaccgccc gccggcgcau cgagcggcgc cggaaccgca ucagcuaccu ccaggggauc      360

uucgccgagg agauguccaa gaccgacgcc aacuucuucu gccggcugag cgacuccuuc      420

uacguggaca acgagaagcg caacagccgg caccccuucu ucgcgacgau cgaggaggag      480

gucgaguacc acaagaacua ccccaccauc uaccaccucc gcgaggagcu ggugaacucc      540

agcgagaagg ccgaccugcg gcugguguac cucgcccugg cccacaucau caaguaccgc      600

ggcaacuucc ugaucgaggg ggcccucgac acccagaaca ccuccgucga cggcaucuac      660

aagcaguuca uccagacgua caaccaggug uucgcgagcg ggaucgagga cggcucccug      720

aagaagcugg aggacaacaa ggacguggcc aagauccucg ucgagaaggu gacccggaag      780

gagaagcugg agcgcauccu gaagcucuac ccgggcgaga aguccgccgg gauguucgcc      840

caguucauca gccugaucgu gggcagcaag gggaacuucc agaagcccuu cgaccugauc      900

gagaaguccg acaucgagug cgccaaggac agcuacgagg aggaccucga gucccugcug      960

gcgcucaucg gcgacgagua cgccgagcug uucgucgccg ccaagaacgc cuacagcgcg     1020

guggugcugu ccagcaucau caccgucgcc gagaccgaga cgaacgccaa gcucagcgcc     1080

uccaugaucg agcgguucga cacccacgag gaggaccugg gcgagcugaa ggccuucauc     1140

aagcuccacc uccccaagca cuacgaggag aucuucagca acaccgagaa gcacggguac     1200

gcgggcuaca ucgacgggaa gaccaagcag gccgacuucu acaaguacau gaagaugacg     1260

cuggagaaca ucgagggcgc cgacuacuuc aucgccaaga ucgagaagga gaacuuccug     1320

cgcaagcagc gcaccuucga caacggggcg aucccccacc agcugcaccu ggaggagcuc     1380

gaggccaucc ugcaccagca ggccaaguac uacccguucc ugaaggagaa cuacgacaag     1440

aucaaguccc ucgugaccuu ccggaucccc uacuucgugg gcccccuggc caacggccag     1500

agcgaguucg ccuggcugac ccgcaaggcg gacggggaga uccggcccug gaacaucgag     1560

gagaaggucg acuucggcaa guccgccgug gacuucaucg agaagaugac gaacaaggac     1620

accuaccucc cgaaggagaa cgugcugccc aagcacagcc ugugcuacca gaaguaccuc     1680

gucuacaacg agcugaccaa ggugcgcuac aucaacgacc aggggaagac cuccuacuuc     1740

agcggccagg agaaggagca gaucuucaac gaccuguuca agcagaagcg gaaggucaag     1800

aagaaggacc ucgagcuguu ccugcgcaac augucccacg uggagagccc cacgaucgag     1860

gggcuggagg acuccuucaa cagcuccuac agcaccuacc acgaccuccu gaaggugggc     1920

aucaagcagg agauccugga caaccccguc aacaccgaga ugcucgagaa caucgugaag     1980

auccugaccg uguucgagga caagcggaug aucaaggagc agcugcagca guucuccgac     2040

guccucgacg gcguggugcu gaagaagcug gagcgccggc acuacacggg guggggccgc     2100

cucagcgcca agcugcugau ggggauccgg gacaagcagu cccaccucac cauccuggac     2160

uaccugauga acgacgacgg ccucaaccgc aaccugaugc agcugaucaa cgacagcaac     2220

cucuccuuca agagcaucau cgagaaggag caggucacca ccgccgacaa ggacauccag     2280

agcaucgugg ccgaccuggc gggguccccg gccaucaaga agggcauccu gcagagccuc     2340

aagaucgugg acgagcuggu cuccgugaug ggcuacccgc cccagacgau cguggucgag     2400

auggcccggg agaaccagac caccgggaag ggcaagaaca acagccgccc ccgguacaag     2460

ucccuggaga aggccaucaa ggaguucggg agccagaucc ucaaggagca ccccaccgac     2520

aaccaggagc ugcgcaacaa ccggcuguac cucuacuacc ugcagaacgg caaggacaug     2580

uacacgggcc aggaccugga cauccacaac cucuccaacu acgacaucga ccacaucgug     2640

ccccagagcu ucaucaccga caacuccauc gacaaccugg ugcugaccag cuccgccggg     2700

aaccgcgaga agggcgacga cgucccgccc cucgagaucg ugcggaagcg caagguguuc     2760

ugggagaagc uguaccaggg gaaccugaug agcaagcgca aguucgacua ccucaccaag     2820

gcggagcggg gcgggcugac cgaggccgac aaggcccgcu ucauccaccg gcagcugguc     2880

gagacgcgcc agaucaccaa gaacguggcc aacauccucc accagcgguu caacuacgag     2940

aaggacgacc acggcaacac caugaagcag guccgcaucg ugacccugaa guccgcgcug     3000

gugagccagu uccggaagca guuccagcuc uacaaggugc gcgacgucaa cgacuaccac     3060

cacgcccacg acgccuaccu gaacggcgug guggccaaca cgcugcucaa ggucuacccc     3120

cagcuggagc ccgaguucgu guacggggac uaccaccagu ucgacugguu caaggccaac     3180

aaggcgaccg ccaagaagca guucuacacc aacaucaugc uguucuucgc ccagaaggac     3240

cggaucaucg acgagaacgg cgagauccuc ugggacaaga aguaccugga caccgugaag     3300

aaggucaugu ccuaccgcca gaugaacauc gugaagaaga cggagaucca gaagggggag     3360

uucagcaagg ccaccaucaa gcccaagggc aacuccagca agcugauccc ccggaagacc     3420

aacugggacc cgaugaagua cgggggccuc gacagcccca acauggccua cgcggugguc     3480

aucgaguacg ccaagggcaa gaacaagcug guguucgaga agaagaucau ccgcgugacc     3540

aucauggagc ggaaggccuu cgagaaggac gagaaggccu uccuggagga gcagggguac     3600

cgccagccca agguccucgc caagcugccg aaguacacgc uguacgagug cgaggagggc     3660

cggcgccgga ugcucgcguc cgccaacgag gcccagaagg ggaaccagca gguccugccc     3720

aaccaccugg ugacccuccu gcaccacgcc gcgaacugcg aggugagcga cggcaagucc     3780

cuggacuaca ucgagagcaa ccgcgagaug uucgccgagc uccuggccca cguguccgag     3840

uucgccaagc gguacacccu ggccgaggcg aaccucaaca agaucaacca gcuguucgag     3900

cagaacaagg agggggacau caaggccauc gcccagagcu ucguggaccu gauggccuuc     3960

aacgccaugg gcgcgcccgc cuccuucaag uucuucgaga ccacgaucga gcgcaagcgg     4020

uacaacaacc ucaaggagcu gcugaacagc accaucaucu accaguccau caccggccuc     4080

uacgagagcc gcaagcgccu ggacgacaag cggcccgccg ccaccaagaa ggccgggcag     4140

gcgaagaaga agaaguaagg acuagugcau cacauuuaaa agcaucucag ccuaccauga     4200

gaauaagaga aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu     4260

guaaagccaa cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc     4320

ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  4428


<210>  2418
<211>  4461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2418
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgaa gaagcccuac      120

agcaucggcc uggacaucgg gaccaacucc gugggcuggg ccguggucac cgacgacuac      180

aaggugcccg ccaagaagau gaaggugcuc gggaacacgg acaagagcca caucgagaag      240

aaccugcugg gcgcccuccu guucgacucc ggcaacaccg ccgaggaccg gcgccugaag      300

cggaccgcgc gccggcgcua cacccggcgc cggaaccgca uccucuaccu gcaggagauc      360

uucagcgagg agauggggaa ggucgacgac uccuucuucc accggcugga ggacagcuuc      420

cucgugacgg aggacaagcg cggcgagcgg caccccaucu ucgggaaccu ggaggaggag      480

gugaaguacc acgagaacuu cccgaccauc uaccaccucc gccaguaccu ggccgacaac      540

cccgagaagg ucgaccugcg gcugguguac cucgcccugg cccacaucau caaguuccgc      600

ggccacuucc ugaucgaggg gaaguucgac acccggaaca acgacgugca gcgccucuuc      660

caggaguucc uggcggucua cgacaacacc uucgagaacu ccagccugca ggagcagaac      720

guccaggugg aggagauccu cacggacaag aucuccaaga gcgccaagaa ggaccgggug      780

cugaagcugu uccccaacga gaaguccaac ggccgcuucg ccgaguuccu caagcugauc      840

gugggcaacc aggccgacuu caagaagcac uucgagcugg aggagaaggc cccgcuccag      900

uucagcaagg acaccuacga ggaggagcug gaggugcugc ucgcgcagau cggggacaac      960

uacgccgagc uguuccuguc cgccaagaag cucuacgaca gcauccugcu guccggcauc     1020

cucaccguca ccgacguggg gacgaaggcc ccgcugagcg ccuccaugau ccagcgguac     1080

aacgagcacc agauggaccu ggcgcagcuc aagcaguuca uccgccagaa gcugagcgac     1140

aaguacaacg agguguucuc cgacgucagc aaggacggcu acgccggcua caucgacggg     1200

aagaccaacc aggaggccuu cuacaaguac cugaagggcc uccugaacaa gaucgagggg     1260

agcggcuacu uccuggacaa gaucgagcgc gaggacuucc uccggaagca gcgcaccuuc     1320

gacaacgggu ccauccccca ccagauccac cuccaggaga ugcgggccau cauccgccgg     1380

caggccgagu ucuaccccuu ccuggcggac aaccaggacc gcaucgagaa gcugcugacc     1440

uuccggaucc ccuacuacgu gggcccgcug gcccgcggca agagcgacuu cgccuggcug     1500

ucccgcaaga gcgccgacaa gaucacgccc uggaacuucg acgagaucgu ggacaaggag     1560

uccagcgccg aggcguucau caaccggaug accaacuacg accucuaccu gcccaaccag     1620

aagguccugc ccaagcacuc ccuccuguac gagaaguuca ccguguacaa cgagcugacc     1680

aaggucaagu acaagacgga gcaggggaag accgccuucu ucgacgccaa caugaagcag     1740

gagaucuucg acggcguguu caagguguac cggaagguca ccaaggacaa gcucauggac     1800

uuccuggaga aggaguucga cgaguuccgc aucguggacc ugaccgggcu cgacaaggag     1860

aacaaggugu ucaacgccag cuacggcacg uaccacgacc ugugcaagau ccuggacaag     1920

gacuuccucg acaacuccaa gaacgagaag auccuggagg acaucguccu gacccucacc     1980

cuguucgagg accgggagau gauccgcaag cggcuggaga acuacagcga ccuccugacc     2040

aaggagcagg ucaagaagcu ggagcgccgg cacuacacgg gguggggccg ccucuccgcg     2100

gagcugaucc acggcauccg gaacaaggag agccgcaaga ccauccugga cuaccucauc     2160

gacgacggga acuccaaccg caacuucaug cagcugauca acgacgacgc ccugagcuuc     2220

aaggaggaga ucgccaaggc ccaggugauc ggcgagaccg acaaccucaa ccagguggug     2280

uccgacaucg ccgggagccc ggcgaucaag aagggcaucc ugcagucccu gaagaucgug     2340

gacgagcucg ucaagaucau ggggcaccag cccgagaaca ucguggugga gauggcccgg     2400

gagaaccagu ucaccaacca gggccgccgg aacagccagc agcgccugaa gggccugacg     2460

gacuccauca aggaguucgg gagccagauc cucaaggagc accccgucga gaacagccag     2520

cugcagaacg accggcuguu ccucuacuac cugcagaacg gccgcgacau guacaccggg     2580

gaggagcugg acaucgacua ccucucccag uacgacaucg accacaucau cccccaggcc     2640

uucaucaagg acaacagcau cgacaaccgg gugcugaccu ccagcaagga gaaccgcggc     2700

aaguccgacg acgugccgag caaggacguc gugcggaaga ugaaguccua cuggagcaag     2760

cugcucuccg ccaagcugau cacccagcgc aaguucgaca accugacgaa ggccgagcgg     2820

ggcgggcuca ccgacgacga caaggcgggc uucaucaagc gccagcuggu ggagacccgg     2880

cagaucacca agcacgucgc ccgcauccug gacgagcggu ucaacaccga gacggacgag     2940

aacaacaaga agauccgcca ggugaagauc gugacccuca agagcaaccu ggucuccaac     3000

uuccggaagg aguucgagcu guacaaggug cgcgagauca acgacuacca ccacgcccac     3060

gacgccuacc ucaacgccgu gaucgggaag gcgcugcugg gcgucuaccc ccagcucgag     3120

cccgaguucg uguacgggga cuacccccac uuccacggcc acaaggagaa caaggccacc     3180

gccaagaagu ucuucuacag caacaucaug aacuucuuca agaaggacga cgugcggacc     3240

gacaagaacg gcgagaucau cuggaagaag gacgagcaca ucuccaacau caagaagguc     3300

cugagcuacc cccaggugaa caucgugaag aaggucgagg agcagaccgg gggcuucucc     3360

aaggagagca uccugcccaa ggggaacucc gacaagcuca ucccccgcaa gacgaagaag     3420

uucuacuggg acaccaagaa guacggcggg uucgacagcc ccaucguggc cuacagcauc     3480

cuggugaucg cggacaucga gaagggcaag uccaagaagc ugaagaccgu caaggcccuc     3540

gugggcguga cgaucaugga gaagaugacc uucgagcggg acccggucgc cuuccuggag     3600

cgcaaggggu accgcaacgu gcaggaggag aacaucauca agcugcccaa guacagccuc     3660

uucaagcugg agaacggccg gaagcgccug cucgccuccg cccgggagcu gcagaagggg     3720

aacgagaucg ugcugcccaa ccaccucggc acccugcugu accacgcgaa gaacauccac     3780

aaggucgacg agccgaagca ccucgacuac guggacaagc acaaggacga guucaaggag     3840

cugcuggacg uggucagcaa cuucuccaag aaguacaccc ucgccgaggg gaaccuggag     3900

aagaucaagg agcuguacgc ccagaacaac ggcgaggacc ucaaggagcu ggccagcucc     3960

uucaucaacc ugcucacguu caccgccauc ggcgcgcccg ccaccuucaa guucuucgac     4020

aagaacaucg accgcaagcg guacaccagc acgaccgaga uccugaacgc cacccugauc     4080

caccagucca ucaccgggcu cuacgagacg cgcaucgacc ugaacaagcu gggcggggac     4140

aagcggcccg ccgccaccaa gaaggcgggc caggccaaga agaagaagua aggacuagug     4200

caucacauuu aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa     4260

uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac     4320

auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa     4380

gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaagaau u                                               4461


<210>  2419
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2419
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgca gaccaccaac      120

cugagcuaca uccucggccu ggaccugggg aucgccuccg ugggcugggc cguggucgag      180

aucaacgaga acgaggaccc caucgggcuc aucgacgugg gcgugcggau cuucgagcgc      240

gccgaggucc ccaagacggg cgagagccug gcccuguccc ggcgccucgc gcggagcacc      300

cgccggcuga uccgccggcg cgcccaccgg cugcuccugg ccaagcgcuu ccugaagcgg      360

gaggggaucc ucuccaccau cgaccuggag aagggccugc ccaaccaggc cugggagcuc      420

cgcguggcgg ggcuggagcg gcgccugagc gccaucgagu ggggcgccgu gcuccugcac      480

cugaucaagc accgggggua ccucuccaag cgcaagaacg agagccagac caacaacaag      540

gagcugggcg cccugcucuc cggcgucgcc cagaaccacc agcugcugca gagcgacgac      600

uaccggacgc cggcggagcu cgcccugaag aaguucgcca aggaggaggg gcacauccgc      660

aaccagcggg gcgccuacac ccacaccuuc aaccgccugg accuccuggc cgagcugaac      720

cuccuguucg cgcagcagca ccaguucggg aacccccacu gcaaggagca cauccagcag      780

uacaugaccg agcugcucau guggcagaag cccgcccugu ccggcgaggc cauccugaag      840

augcucggca agugcacgca cgagaagaac gaguucaagg ccgccaagca caccuacagc      900

gcggagcgcu ucguguggcu gaccaagcug aacaaccucc ggauccugga ggacggggcc      960

gagcgcgccc ugaacgagga ggagcggcag cuccugauca accaccccua cgagaagucc     1020

aagcugaccu acgcccaggu gcgcaagcuc cugggccuga gcgagcaggc caucuucaag     1080

caccuccggu acuccaagga gaacgcggag agcgccacgu ucauggagcu caaggccugg     1140

cacgccaucc gcaaggcgcu ggagaaccag gggcugaagg acaccuggca ggaccucgcc     1200

aagaagccgg accugcugga cgagaucggc accgccuucu cccucuacaa gaccgacgag     1260

gacauccagc aguaccugac gaacaagguc cccaacagcg ugaucaacgc ccugcucgug     1320

ucccugaacu ucgacaaguu caucgagcug agccucaaga gccugcggaa gauccugccc     1380

cucauggagc aggggaagcg cuacgaccag gccugccggg agaucuacgg ccaccacuac     1440

ggcgaggcga accagaagac cucccagcug cugcccgcca ucccggccca ggagauccgc     1500

aaccccgucg ugcuccggac ccugagccag gcccgcaagg ugaucaacgc caucauccgg     1560

caguacgggu cccccgcgcg cguccacauc gagaccggcc gggagcuggg caagagcuuc     1620

aaggagcgcc gggagaucca gaagcagcag gaggacaacc gcacgaagcg ggaguccgcc     1680

gugcagaagu ucaaggagcu guucagcgac uucuccagcg agcccaaguc caaggacauc     1740

cugaaguucc gccuguacga gcagcagcac gggaagugcc ucuacagcgg gaaggagauc     1800

aacauccacc gccugaacga gaagggcuac guggagaucg accacgcccu gccguucucc     1860

cggaccuggg acgacagcuu caacaacaag guccucgugc uggccuccga gaaccagaac     1920

aagggcaacc agacccccua cgaguggcug caggggaaga ucaacagcga gcgcuggaag     1980

aacuucgugg cccucguccu gggcucccag ugcagcgcgg ccaagaagca gcggcugcuc     2040

acccagguga ucgacgacaa caaguucauc gaccgcaacc ugaacgacac gcgguacauc     2100

gcccgcuucc uguccaacua cauccaggag aaccuccugc uggucgggaa gaacaagaag     2160

aacguguuca cccccaacgg ccagaucacc gcccuccugc ggagccgcug ggggcugauc     2220

aaggcgcggg agaacaacaa ccgccaccac gcccucgacg ccaucguggu cgccugcgcc     2280

acccccucca ugcagcagaa gaucacgcgg uucauccgcu ucaaggaggu gcacccguac     2340

aagaucgaga accgguacga gaugguggac caggagagcg gcgagaucau cagcccccac     2400

uuccccgagc ccugggcgua cuuccgccag gaggucaaca uccggguguu cgacaaccac     2460

ccggacaccg ugcugaagga gaugcugccc gaccgccccc aggccaacca ccaguucguc     2520

cagccccucu ucgugucccg ggccccgacc cgcaagauga gcggccaggg gcacauggag     2580

accaucaagu ccgccaagcg gcuggccgag ggcaucagcg ugcugcgcau cccccucacg     2640

cagcugaagc ccaaccugcu cgagaacaug gucaacaagg agcgggagcc ggcgcuguac     2700

gccgggcuga aggcccgccu cgccgaguuc aaccaggacc ccgccaaggc guucgccacc     2760

cccuucuaca agcagggcgg ccagcagguc aaggccaucc gcguggagca gguccagaag     2820

uccggggugc uggugcggga gaacaacggc guggccgaca acgcgagcau cgugcgcacc     2880

gacguguuca ucaagaacaa caaguucuuc cuggucccca ucuacaccug gcaggucgcc     2940

aaggggaucc ucccgaacaa ggccaucgug gcccacaaga acgaggacga gugggaggag     3000

auggacgagg gcgccaaguu caaguucucc cuguucccca acgaccuggu ggagcucaag     3060

acgaagaagg aguacuucuu cggguacuac aucggccugg accgggcgac cggcaacauc     3120

agccugaagg agcacgacgg ggagaucucc aagggcaagg acggggugua ccgcgugggc     3180

gucaagcucg cccugagcuu cgagaaguac cagguggacg agcuggggaa gaaccggcag     3240

aucugccgcc cccagcagcg gcagcccgug cgcaagcggc cggccgccac caagaaggcc     3300

ggccaggcga agaagaagaa guaaggacua gugcaucaca uuuaaaagca ucucagccua     3360

ccaugagaau aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc     3420

guugguguaa agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu     3480

uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           3594


<210>  2420
<211>  3672
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2420
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcggc cgccuucaag      120

cccaacccca ucaacuacau ccugggccuc gacaucggga ucgccagcgu gggcugggcc      180

augguggaga ucgacgagga cgagaacccc aucugccuga ucgaccuggg gguccgggug      240

uucgagcgcg cggaggugcc gaagaccggc gacucccucg ccauggcccg gcgccuggcc      300

cggagcgucc gccggcugac ccgccggcgc gcgcaccggc uccugcgcgc ccggcgccug      360

cucaagcggg agggcgugcu gcaggccgcc gacuucgacg agaacgggcu gaucaagucc      420

cuccccaaca cgcccuggca gcugcgcgcc gcggcccugg accggaagcu cacgccccug      480

gaguggagcg ccgugcugcu ccaccugauc aagcaccgcg gcuaccuguc ccagcggaag      540

aacgaggggg agaccgccga caaggagcuc ggcgcccugc ugaagggggu cgcggacaac      600

gcccacgccc uccagaccgg cgacuuccgc accccggccg agcuggcccu gaacaaguuc      660

gagaaggaga gcggccacau ccggaaccag cgcggggacu acucccacac cuucagccgc      720

aaggaccucc aggcggagcu gauccuccuc uucgagaagc agaaggaguu cggcaacccc      780

cacguguccg ggggccugaa ggagggcauc gagacccugc ucaugaccca gcggcccgcc      840

cugagcgggg acgccgugca gaagaugcug ggccacugca cguucgagcc cgccgagccg      900

aaggccgcga agaacaccua caccgccgag cgcuucaucu ggcucaccaa gcugaacaac      960

cugcggaucc ucgagcaggg guccgagcgc ccccugacgg acaccgagcg ggccacccug     1020

auggacgagc ccuaccgcaa gagcaagcuc accuacgccc aggcgcggaa gcugcugggc     1080

cucgaggaca cggccuucuu caaggggcug cgcuacggca aggacaacgc cgaggccucc     1140

acccugaugg agaugaaggc cuaccacgcg aucagccggg cccucgagaa ggagggccug     1200

aaggacaaga aguccccgcu gaaccugagc cccgagcucc aggacgagau cgggaccgcc     1260

uucucccugu ucaagaccga cgaggacauc acgggccgcc ugaaggaccg gauccagccc     1320

gagauccucg aggcccugcu gaagcacauc agcuucgaca aguucgucca gaucucccuc     1380

aaggcccugc gccggaucgu gccccugaug gagcagggga agcgcuacga cgaggcgugc     1440

gccgagaucu acggcgacca cuacgggaag aagaacaccg aggagaagau cuaccugccc     1500

ccgauccccg ccgacgagau ccggaacccc gugguccugc gcgcccugag ccaggcccgg     1560

aaggugauca acggcguggu ccgccgguac ggcagccccg cgcgcaucca caucgagacc     1620

gcccgcgagg uggggaaguc cuucaaggac cggaaggaga ucgagaagcg ccaggaggag     1680

aaccggaagg accgcgagaa ggccgccgcg aaguuccggg aguacuuccc gaacuucgug     1740

ggcgagccca agagcaagga cauccucaag cugcgccugu acgagcagca gcacgggaag     1800

ugccucuacu ccggcaagga gaucaaccug gggcggcuga acgagaaggg cuacgucgag     1860

aucgaccacg cccuccccuu cagccgcacc ugggacgacu ccuucaacaa caaggugcug     1920

gugcugggca gcgagaacca gaacaagggg aaccagacgc ccuacgagua cuucaacggc     1980

aaggacaacu cccgggagug gcaggaguuc aaggcccgcg ucgagaccag ccgguucccg     2040

cgcuccaaga agcagcggau ccuccugcag aaguucgacg aggacggguu caaggagcgc     2100

aaccugaacg acacccggua cgugaaccgc uuccucugcc aguucgucgc cgaccggaug     2160

cgccugaccg gcaagggcaa gaagcgggug uucgccagca acgggcagau cacgaaccug     2220

cuccgcggcu ucugggggcu gcggaaggug cgcgcggaga acgaccgcca ccacgcccug     2280

gacgccgucg ugguggccug cuccaccguc gccaugcagc agaagaucac ccgguucgug     2340

cgcuacaagg agaugaacgc guucgacggc aagaccaucg acaaggagac gggggaggug     2400

cuccaccaga agacccacuu cccccagccc ugggaguucu ucgcccagga ggucaugauc     2460

cggguguucg gcaagccgga cggcaagccc gaguucgagg aggccgacac ccccgagaag     2520

cugcgcaccc ugcucgccga gaagcugagc ucccggcccg aggccgugca cgaguacguc     2580

acgccgcugu ucgugagccg cgcgcccaac cggaagaugu ccgggcaggg ccacauggag     2640

accgucaaga gcgccaagcg ccucgacgag gggguguccg ugcugcgggu gccccugacc     2700

cagcucaagc ugaaggaccu ggagaagaug gucaaccgcg agcgggagcc caagcucuac     2760

gaggcccuga aggcccgccu ggaggcgcac aaggacgacc cggccaaggc cuucgccgag     2820

cccuucuaca aguacgacaa ggccggcaac cggacccagc aggucaaggc ggugcgcgug     2880

gagcaggucc agaagaccgg ggugugggug cggaaccaca acggcaucgc cgacaacgcc     2940

acgauggugc gcguggacgu cuucgagaag ggcgacaagu acuaccucgu gcccaucuac     3000

agcuggcagg ucgccaaggg gauccugccc gaccgggccg uggugcaggg caaggacgag     3060

gaggacuggc agcugaucga cgacuccuuc aacuucaagu ucagccucca cccgaacgac     3120

cugguggagg ucaucaccaa gaaggcgcgc auguucgggu acuucgccag cugccaccgg     3180

ggcaccggga acaucaacau ccgcauccac gaccuggacc acaagaucgg caagaacggc     3240

auccucgagg ggaucggcgu gaagaccgcc cuguccuucc agaaguacca gaucgacgag     3300

cuggggaagg agauccgccc cugccggcuc aagaagcgcc cgcccgugcg gaagcgcccc     3360

gccgccacga agaaggcggg ccaggccaag aagaagaagu aaggacuagu gcaucacauu     3420

uaaaagcauc ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu     3480

caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc     3540

uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga     3600

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaagaa uu                                                         3672


<210>  2421
<211>  3678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2421
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgaa guaccacgug      120

ggcaucgacg uggggaccuu cagcgucggc cuggccgcca ucgaggugga cgacgccggg      180

augcccauca agacccuguc ccuggugagc cacauccacg acuccggccu ggaccccgac      240

gagaucaaga gcgccgucac gcggcucgcg uccagcggca ucgcccgccg gacccgccgg      300

cuguaccgcc ggaagcgccg gcgccugcag cagcucgaca aguucaucca gcggcagggg      360

uggcccguga ucgagcugga ggacuacucc gacccgcugu accccuggaa ggugcgcgcc      420

gagcucgccg cgagcuacau cgccgacgag aaggagcggg gcgagaagcu guccgucgcc      480

cugcgccaca ucgcccggca ccgcgggugg cggaaccccu acgccaaggu gagcucccuc      540

uaccugcccg acggcccgag cgacgcguuc aaggccaucc gcgaggagau caagcgggcc      600

uccgggcagc ccgugcccga gaccgccacc gucggccaga uggugacgcu gugcgagcuc      660

ggcacccuga agcugcgcgg ggagggcggg gugcucagcg cccggcugca gcaguccgac      720

uacgcgcgcg agauccagga gaucugccgc augcaggaga ucggccagga gcuguaccgg      780

aagaucaucg acgucguguu cgccgccgag agccccaagg gcuccgccag cucccgcgug      840

gggaaggacc cgcuccagcc cggcaagaac cgggcccuga aggcgagcga cgccuuccag      900

cgcuaccgga ucgccgcccu gaucgggaac cuccgcgucc ggguggacgg cgagaagcgc      960

auccugagcg uggaggagaa gaaccugguc uucgaccacc ucgugaaccu gacccccaag     1020

aaggagcccg agugggugac caucgccgag auccugggga ucgaccgggg ccagcucauc     1080

ggcacggcga ccaugaccga cgacggggag cgcgccggcg cccggccgcc cacccacgac     1140

acgaaccgcu ccaucgucaa cagccggauc gcgccccugg uggacuggug gaagaccgcc     1200

uccgcccugg agcagcacgc cauggucaag gcccucagca acgccgaggu ggacgacuuc     1260

gacucccccg agggggcgaa ggugcaggcc uucuucgccg accuggacga cgacguccac     1320

gccaagcugg acagccucca ccucccggug ggccgcgccg cguacuccga ggacacccug     1380

gugcggcuca cccgccggau gcugagcgac ggggucgacc uguacacggc ccgccuccag     1440

gaguucggca ucgagcccuc cuggaccccg cccacgcccc ggaucggcga gcccgugggg     1500

aaccccgccg uggaccgcgu ccugaagacc gugagccgcu ggcuggaguc cgccaccaag     1560

accuggggcg cccccgagcg ggugaucauc gagcacgucc gcgagggguu cgugaccgag     1620

aagcgggcgc gcgagaugga cggcgacaug cggcgccggg ccgcccgcaa cgccaagcug     1680

uuccaggaga ugcaggagaa gcugaacgug caggggaagc cgagccgggc ggaccugugg     1740

cgcuaccagu ccguccagcg gcagaacugc cagugcgccu acugcggcag ccccaucacc     1800

uucuccaaca gcgagaugga ccacaucgug ccccgcgccg gccagggguc cacgaacacc     1860

cgggagaacc ucguggccgu cugccaccgc ugcaaccaga gcaagggcaa caccccguuc     1920

gccaucuggg cgaagaacac cuccaucgag ggggugagcg ugaaggaggc cgucgagcgg     1980

acgcgccacu gggugaccga caccggcaug cggagcaccg acuucaagaa guucacgaag     2040

gccguggucg agcgcuucca gcgggccacc auggacgagg agaucgacgc ccgcuccaug     2100

gagagcgugg cguggauggc caacgagcug cggucccgcg uggcccagca cuucgccagc     2160

cacggcacca ccguccgcgu guaccggggg ucccugacgg ccgaggcgcg ccgggccagc     2220

ggcaucuccg ggaagcugaa guucuucgac ggcgugggca agagccgccu cgaccggcgc     2280

caccacgcca ucgacgccgc ggucaucgcc uucaccuccg acuacguggc cgagacccug     2340

gccgugcgga gcaaccugaa gcagucccag gcccaccgcc aggaggcgcc ccaguggcgg     2400

gaguucaccg ggaaggacgc cgagcaccgc gccgccuggc gggucuggug ccagaagaug     2460

gagaagcuca gcgcccugcu gaccgaggac cuccgcgacg accggguggu ggucaugucc     2520

aacgugcgcc ugcggcuggg caacgggagc gcgcacaagg agacgaucgg caagcucucc     2580

aaggugaagc ugagcuccca gcugagcguc agcgacaucg acaaggccuc cagcgaggcc     2640

cucuggugcg cccugacccg cgagcccggg uucgacccca aggagggccu gccggccaac     2700

cccgagcggc acauccgcgu gaacgggacc cacguguacg cgggcgacaa caucggccuc     2760

uuccccgucu ccgccgggag caucgcccug cggggcgggu acgccgagcu gggcuccagc     2820

uuccaccacg cccgcgugua caagaucacc uccgggaaga agcccgcguu cgccaugcuc     2880

cgcguguaca cgaucgaccu gcugccguac cggaaccagg accucuucag cgucgagcug     2940

aagccccaga ccauguccau gcgccaggcc gagaagaagc ugcgggacgc ccucgcgacc     3000

ggcaacgccg aguaccuggg cuggcuggug guggacgacg agcucgucgu ggacaccagc     3060

aagaucgcca cggaccaggu gaaggccguc gaggccgagc uggggaccau ccgccggugg     3120

cgcguggacg gcuucuucuc ccccagcaag cugcggcucc gcccccugca gauguccaag     3180

gaggggauca agaaggagag cgcgccggag cuguccaaga ucaucgaccg gcccggcugg     3240

cuccccgccg ucaacaagcu guucagcgac gggaacguga ccgugguccg ccgggacucc     3300

cugggccgcg ugcggcucga gagcaccgcc caccuccccg ugacguggaa gguccagaag     3360

cgcccggccg ccaccaagaa ggcgggccag gccaagaaga agaaguaagg acuagugcau     3420

cacauuuaaa agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag     3480

cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua     3540

aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa     3600

ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaagaauu                                                   3678


<210>  2422
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2422
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcggc ccggauccug      120

gccuucgaca ucggcaucag cuccaucggg ugggccuuca gcgagaacga cgagcucaag      180

gacugcggcg ugcgcaucuu caccaaggug gagaacccca agaccgggga gucccuggcc      240

cugccccggc gccucgcgcg gagcgcccgc aagcggcugg cccgccggaa ggcccgccug      300

aaccaccuca agcaccugau cgcgaacgag uucaagcuga acuacgagga cuaccagucc      360

uucgacgaga gccucgccaa ggccuacaag ggcucccuga ucagccccua cgagcugcgg      420

uuccgcgccc ucaacgagcu gcuguccaag caggacuucg cccgggucau ccuccacauc      480

gcgaagcgcc ggggcuacga cgacaucaag aacagcgacg acaaggagaa gggggccauc      540

cugaaggcca ucaagcagaa cgaggagaag cuggccaacu accaguccgu gggcgaguac      600

cucuacaagg aguacuucca gaaguucaag gagaacagca aggaguucac gaacgugcgc      660

aacaagaagg aguccuacga gcggugcauc gcccagagcu uccugaagga cgagcugaag      720

cucaucuuca agaagcagcg cgaguucggg uucuccuuca gcaagaaguu cgaggaggag      780

guccuguccg uggcguucua caagcgggcc cugaaggacu ucagccaccu cgugggcaac      840

ugcuccuucu ucaccgacga gaagcgcgcc ccgaagaaca gcccccuggc cuucauguuc      900

gucgcccuga cccggaucau caaccuccug aacaaccuga agaacaccga ggggauccuc      960

uacacgaagg acgaccugaa cgcgcugcuc aacgaggugc ugaagaacgg cacccugacc     1020

uacaagcaga ccaagaagcu ccugggccug agcgacgacu acgaguucaa gggggagaag     1080

ggcacguacu ucaucgaguu caagaaguac aaggaguuca ucaaggcccu cggggagcac     1140

aaccuguccc aggacgaccu gaacgagauc gccaaggaca ucacccucau caaggacgag     1200

aucaagcuga agaaggcccu ggccaaguac gaccucaacc agaaccagau cgacagccug     1260

uccaagcugg aguucaagga ccaccucaac aucagcuuca aggcgcugaa gcuggugacg     1320

ccccugaugc ucgagggcaa gaaguacgac gaggccugca acgagcugaa ccugaagguc     1380

gccaucaacg aggacaagaa ggacuuccuc cccgccuuca acgagaccua cuacaaggac     1440

gaggugacca acccgguggu ccugcgcgcg aucaaggagu accgcaaggu gcugaacgcc     1500

cuccugaaga aguacggcaa ggugcacaag aucaacaucg agcuggcccg ggaggucggg     1560

aagaaccacu cccagcgcgc caagaucgag aaggagcaga acgagaacua caaggccaag     1620

aaggacgcgg agcucgagug cgagaagcug ggccugaaga ucaacagcaa gaacauccuc     1680

aagcugcggc uguucaagga gcagaaggag uucugcgccu acuccgggga gaagaucaag     1740

aucagcgacc uccaggacga gaagaugcug gagaucgacc acaucuaccc cuacucccgc     1800

agcuucgacg acuccuacau gaacaaggug cugguguuca ccaagcagaa ccaggagaag     1860

cucaaccaga cccccuucga ggccuucggc aacgacagcg ccaaguggca gaagaucgag     1920

guccuggcca agaaccugcc caccaagaag cagaagcgga uccucgacaa gaacuacaag     1980

gacaaggagc agaagaacuu caaggaccgc aaccugaacg acacgcggua caucgcgcgc     2040

cuggugcuca acuacaccaa ggacuaccug gacuuccugc cgcucuccga cgacgagaac     2100

accaagcuga acgacaccca gaaggggagc aaggugcacg ucgaggccaa guccggcaug     2160

cugacgagcg cccuccggca caccuggggc uucuccgcca aggaccgcaa caaccaccuc     2220

caccacgcca ucgacgcggu gaucaucgcc uacgccaaca acagcaucgu caaggccuuc     2280

uccgacuuca agaaggagca ggagagcaac agcgcggagc uguacgccaa gaagaucucc     2340

gagcuggacu acaagaacaa gcggaaguuc uucgagcccu ucagcggguu ccgccagaag     2400

gugcuggaca agaucgacga gaucuucgug uccaagcccg agcggaagaa gcccagcggc     2460

gcccugcacg aggagaccuu ccgcaaggag gaggaguucu accaguccua cgggggcaag     2520

gagggggucc ucaaggcccu ggagcugggc aagauccgga aggugaacgg caagaucgug     2580

aagaacgggg acauguuccg cgucgacauc uucaagcaca agaagaccaa caaguucuac     2640

gccgugccga ucuacacgau ggacuucgcg cucaaggugc ugcccaacaa ggccgucgcc     2700

cggagcaaga agggcgagau caaggacugg auccugaugg acgagaacua cgaguucugc     2760

uucucccucu acaaggacag ccugauccug auccagacca aggacaugca ggagcccgag     2820

uucguguacu acaacgccuu caccuccagc accguguccc ucaucgucag caagcacgac     2880

aacaaguucg agacgcuguc caagaaccag aagauccugu ucaagaacgc caacgagaag     2940

gaggugaucg ccaagagcau cgggauccag aaccucaagg uguucgagaa guacaucguc     3000

uccgcgcugg gcgaggugac caaggccgag uuccgccagc gggaggacuu caagaagaag     3060

cgccccgccg ccaccaagaa ggcggggcag gccaagaaga agaaguaagg acuagugcau     3120

cacauuuaaa agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag     3180

cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua     3240

aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa     3300

ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3360

aaaaaaaaaa aaagaauu                                                   3378


<210>  2423
<211>  3789
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2423
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgag cgaccuggug      120

cucggccugg acaucgggau cggcuccgug ggggucggca uccugaacaa ggugaccggc      180

gagaucaucc acaagaacag ccggaucuuc cccgccgccc aggccgagaa caaccucgug      240

cgccggacca accgccaggg gcggcgccug gcccggcgca agaagcaccg gcgcguccgg      300

cugaaccgcc ucuucgagga guccggccug aucacggacu ucaccaagau cagcaucaac      360

cugaaccccu accagcuccg ggugaagggg cugaccgacg agcuguccaa cgaggagcug      420

uucaucgcgc ugaagaacau ggugaagcac cgcggcauca gcuaccugga cgacgccucc      480

gacgacggga acagcuccgu cggcgacuac gcccagaucg ugaaggagaa cagcaagcag      540

cucgagacca agacgcccgg ccagauccag cuggagcggu accagaccua cgggcagcug      600

cgcggcgacu ucaccgugga gaaggacggg aagaagcacc ggcucaucaa cgucuucccg      660

accuccgccu accgcagcga ggcgcugcgg auccugcaga cgcagcagga guucaacccc      720

cagaucaccg acgaguucau caaccgcuac cucgagaucc ugaccggcaa gcggaaguac      780

uaccacggcc ccgggaacga gaagucccgc accgacuacg gccgcuaccg gacgagcggg      840

gagacccugg acaacaucuu cggcauccuc aucgggaagu gcaccuucua ccccgacgag      900

uuccgcgccg ccaaggccuc cuacaccgcc caggaguuca accugcugaa cgaccucaac      960

aaccugacgg ugccgaccga gaccaagaag cugagcaagg agcagaagaa ccagaucauc     1020

aacuacguga agaacgagaa ggcgaugggc cccgccaagc uguucaagua caucgccaag     1080

cuccuguccu gcgacgucgc cgacaucaag ggcuaccgga ucgacaagag cgggaaggcc     1140

gagauccaca ccuucgaggc guaccgcaag augaagacgc ucgagacccu ggacaucgag     1200

cagauggacc gggagacccu ggacaagcuc gccuacgugc ugacccugaa cacggagcgc     1260

gagggcaucc aggaggcccu cgagcacgag uucgccgacg gguccuucag ccagaagcag     1320

gucgacgagc uggugcaguu ccggaaggcc aacagcucca ucuucggcaa gggguggcac     1380

aacuucagcg ugaagcugau gauggagcuc auccccgagc uguacgagac cuccgaggag     1440

cagaugacca uccugacccg ccucggcaag cagaagacga ccagcuccag caacaagacc     1500

aaguacaucg acgagaagcu gcugaccgag gagaucuaca accccguggu cgcgaagucc     1560

gugcggcagg ccaucaagau cgugaacgcc gccaucaagg aguacggcga cuucgacaac     1620

aucgucaucg agauggcccg cgagacgaac gaggacgacg agaagaaggc gauccagaag     1680

auccagaagg ccaacaagga cgagaaggac gccgccaugc ucaaggcggc caaccaguac     1740

aacgggaagg ccgagcugcc gcacagcgug uuccacggcc acaagcagcu ggccaccaag     1800

auccggcugu ggcaccagca gggggagcgc ugccucuaca ccggcaagac caucuccauc     1860

cacgaccuga ucaacaacag caaccaguuc gaggucgacc acauccugcc ccucuccauc     1920

acguucgacg acagccuggc caacaaggug cugguguacg cgaccgccaa ccaggagaag     1980

gggcagcgga cccccuacca ggcccucgac uccauggacg acgccuggag cuuccgcgag     2040

cugaaggccu ucguccggga guccaagacc cugagcaaca agaagaagga guaccuccug     2100

accgaggagg acaucuccaa guucgacgug cgcaagaagu ucaucgagcg gaaccuggug     2160

gacacgcgcu acgcgagccg ggucgugcuc aacgcccugc aggagcacuu ccgcgcccac     2220

aagaucgaca ccaagguguc cgucgugcgc ggccaguuca ccagccagcu gcggcgccac     2280

uggggcaucg agaagacccg ggacacguac caccaccacg ccguggacgc ccucaucauc     2340

gcggccagcu cccagcugaa ccuguggaag aagcagaaga acacccucgu cagcuacucc     2400

gaggaccagc ugcuggacau cgagaccggg gagcucauca gcgacgacga guacaaggag     2460

uccguguuca aggcccccua ccagcacuuc guggacaccc ucaagagcaa ggaguucgag     2520

gacuccaucc uguucagcua ccaggucgac uccaaguuca accgcaagau cagcgacgcc     2580

acgaucuacg cgacccggca ggccaaggug ggcaaggaca aggccgacga gaccuacgug     2640

cucgggaaga ucaaggacau cuacacccag gacggcuacg acgccuucau gaagaucuac     2700

aagaaggaca aguccaaguu ccugauguac cgccacgacc cgcagacguu cgagaagguc     2760

aucgagccca uccuggagaa cuaccccaac aagcagauca acgagaaggg caaggaggug     2820

cccugcaacc cguuccucaa guacaaggag gagcacgggu acauccggaa guacagcaag     2880

aagggcaacg ggcccgagau caagucccug aaguacuacg acagcaagcu gggcaaccac     2940

aucgacauca cccccaagga cuccaacaac aagguggucc uccagagcgu gucccccugg     3000

cgcgccgacg uguacuucaa caagaccacc gggaaguacg agauccuggg ccugaaguac     3060

gcggaccucc aguucgagaa gggcacgggg accuacaaga ucagccagga gaaguacaac     3120

gacaucaaga agaaggaggg cgucgacagc gacuccgagu ucaaguucac ccuguacaag     3180

aacgaccugc uccuggugaa ggacaccgag acgaaggagc agcagcuguu ccgguuccuc     3240

agccgcacca ugccgaagca gaagcacuac guggagcuga agcccuacga caagcagaag     3300

uucgagggcg gggaggcccu gaucaagguc cucgggaacg uggccaacuc cggccagugc     3360

aagaagggcc ugggcaagag caacaucucc aucuacaagg ugcggaccga cguccugggg     3420

aaccagcaca ucaucaagaa cgagggggac aagcccaagc ucgacuucaa gcgccccgcc     3480

gccaccaaga aggcgggcca ggccaagaag aagaaguaag gacuagugca ucacauuuaa     3540

aagcaucuca gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau     3600

cucuuuuucu uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu     3660

aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3780

aaaagaauu                                                             3789


<210>  2424
<211>  4347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2424
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgac ccaguucgag      120

ggcuucacca accuguacca ggugagcaag acgcuccggu ucgagcugau cccccagggg      180

aagacccuga agcacaucca ggagcagggc uucaucgagg aggacaaggc ccgcaacgac      240

cacuacaagg agcucaagcc caucaucgac cggaucuaca agaccuacgc cgaccagugc      300

cugcagcugg ugcagcucga cugggagaac cuguccgccg ccaucgacag cuaccgcaag      360

gagaagaccg aggagacgcg gaacgcgcug aucgaggagc aggccaccua ccgcaacgcc      420

auccacgacu acuucaucgg gcggaccgac aaccucaccg acgccaucaa caagcgccac      480

gcggagaucu acaagggccu guucaaggcc gagcuguuca acggcaaggu ccucaagcag      540

cuggggacgg ugaccaccac cgagcacgag aacgcccugc uccgguccuu cgacaaguuc      600

acgaccuacu ucagcggcuu cuacgagaac cgcaagaacg uguucuccgc cgaggacauc      660

agcaccgcca ucccccaccg gaucguccag gacaacuucc cgaaguucaa ggagaacugc      720

cacaucuuca cccgccugau cacggcggug cccucccugc gggagcacuu cgagaacgug      780

aagaaggcca ucgggaucuu cgucagcacc uccaucgagg agguguucag cuuccccuuc      840

uacaaccagc uccugaccca gacccagauc gaccuguaca accagcuccu gggcgggauc      900

ucccgcgagg ccggcacgga gaagaucaag ggccugaacg aggugcucaa ccuggccauc      960

cagaagaacg acgagaccgc ccacaucauc gcgagccugc cccaccgguu caucccccuc     1020

uucaagcaga uccuguccga ccgcaacacc cugagcuuca uccucgagga guucaagucc     1080

gacgaggagg ucauccagag cuucugcaag uacaagaccc ugcugcggaa cgagaacgug     1140

cucgagacgg ccgaggcccu guucaacgag cugaacucca ucgaccucac ccacaucuuc     1200

aucagccaca agaagcugga gaccaucucc agcgcccugu gcgaccacug ggacacccuc     1260

cgcaacgccc uguacgagcg gcgcaucagc gagcugacgg ggaagaucac caaguccgcg     1320

aaggagaagg ugcagcggag ccucaagcac gaggacauca accugcagga gaucaucucc     1380

gccgccggca aggagcugag cgaggccuuc aagcagaaga ccuccgagau ccucagccac     1440

gcccacgcgg cccuggacca gccgcugccc accacgcuca agaagcagga ggagaaggag     1500

auccugaagu cccagcugga cagccugcuc gggcuguacc accuccucga cugguucgcc     1560

gucgacgagu ccaacgaggu ggaccccgag uucagcgccc gccugaccgg caucaagcug     1620

gagauggagc cgucccucag cuucuacaac aaggcgcgca acuacgccac caagaagccc     1680

uacuccgugg agaaguucaa gcugaacuuc cagaugccca cccuggccag cggcugggac     1740

gucaacaagg agaagaacaa cggggccauc cucuucguga agaacggccu guacuaccug     1800

gggaucaugc ccaagcagaa gggccgguac aaggcccucu ccuucgagcc gacggagaag     1860

accagcgagg gguucgacaa gauguacuac gacuacuucc ccgacgcggc caagaugauc     1920

cccaagugcu ccacccagcu gaaggccgug accgcccacu uccagaccca cacgaccccc     1980

auccugcuca gcaacaacuu caucgagccg cuggagauca ccaaggagau cuacgaccug     2040

aacaaccccg agaaggagcc caagaaguuc cagaccgccu acgcgaagaa gacgggcgac     2100

cagaagggcu accgcgaggc ccucugcaag uggaucgacu ucacccggga cuuccugucc     2160

aaguacacca agaccacgag caucgaccug agcucccucc gccccagcuc ccaguacaag     2220

gaccuggggg aguacuacgc cgagcugaac ccgcuccugu accacaucag cuuccagcgg     2280

aucgccgaga aggagaucau ggacgccguc gagaccggca agcuguaccu cuuccagauc     2340

uacaacaagg acuucgcgaa ggggcaccac ggcaagccca accugcacac ccuguacugg     2400

accggccucu ucucccccga gaaccuggcc aagacgagca ucaagcugaa cgggcaggcc     2460

gagcuguucu accgccccaa gucccggaug aagcgcaugg cccaccggcu gggcgagaag     2520

augcugaaca agaagcucaa ggaccagaag accccgaucc ccgacacccu guaccaggag     2580

cuguacgacu acgugaacca ccgccucagc cacgaccugu ccgacgaggc gcgggcccug     2640

cuccccaacg ugaucaccaa ggaggucagc cacgagauca ucaaggaccg ccgguucacg     2700

uccgacaagu ucuucuucca cgugccgauc acccugaacu accaggccgc caacagcccc     2760

uccaaguuca accagcgcgu caacgccuac cugaaggagc accccgagac ccccaucauc     2820

gggaucgacc ggggcgagcg caaccucauc uacaucaccg ugaucgacag cacggggaag     2880

auccuggagc agcggucccu gaacaccauc cagcaguucg acuaccagaa gaagcucgac     2940

aaccgcgaga aggagcgggu ggcggcccgc caggccugga gcgucguggg caccaucaag     3000

gaccugaagc agggguaccu gucccaggug auccacgaga ucgucgaccu caugauccac     3060

uaccaggccg uggugguccu ggagaaccug aacuucggcu ucaagagcaa gcgcaccggc     3120

aucgccgaga aggcggugua ccagcaguuc gagaagaugc ucaucgacaa gcugaacugc     3180

cuggugcuca aggacuaccc ggccgagaag gucgggggcg ugcugaaccc cuaccagcug     3240

acggaccagu ucaccagcuu cgccaagaug gggacccagu ccggcuuccu cuucuacgug     3300

cccgcccccu acaccagcaa gaucgacccg cugacgggcu ucgucgaccc cuucgugugg     3360

aagaccauca agaaccacga gucccggaag cacuuccugg agggguucga cuuccuccac     3420

uacgacguga agaccggcga cuucauccug cacuucaaga ugaaccgcaa ccugagcuuc     3480

cagcgggggc uccccggcuu caugcccgcc ugggacaucg ucuucgagaa gaacgagacc     3540

caguucgacg cgaaggggac gccguucauc gccggcaagc gcaucgugcc cgugaucgag     3600

aaccaccggu ucaccggccg cuaccgggac cuguaccccg ccaacgagcu gaucgcccuc     3660

cuggaggaga aggggaucgu cuuccgcgac ggcuccaaca uccugcccaa gcuccuggag     3720

aacgacgaca gccacgcgau cgacaccaug guggcccuga uccgguccgu gcuccagaug     3780

cgcaacagca acgccgccac cggggaggac uacaucaacu ccccgguccg ggaccugaac     3840

ggcgugugcu ucgacagccg cuuccagaac cccgaguggc ccauggacgc cgacgcgaac     3900

ggggccuacc acaucgcccu gaagggccag cuccugcuga accaccucaa ggaguccaag     3960

gaccugaagc ugcagaacgg caucagcaac caggacuggc ucgccuacau ccaggagcug     4020

cggaacaagc gccccgccgc gacgaagaag gccgggcagg ccaagaagaa gaaguaagga     4080

cuagugcauc acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa     4140

gaucaauagc uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua     4200

aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa     4260

uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aagaauu                                         4347


<210>  2425
<211>  4326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2425
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga agaagcgcaa ggugggcauc cacggggucc cggccgcgag caucuaccag      120

gaguucguga acaaguacuc ccugagcaag acccuccggu ucgagcugau cccccagggc      180

aagacccugg agaacaucaa ggcccgcggg cucauccugg acgacgagaa gcgggccaag      240

gacuacaaga aggccaagca gaucaucgac aaguaccacc aguucuucau cgaggagauc      300

cuguccagcg ugugcaucuc cgaggaccuc cugcagaacu acagcgacgu cuacuucaag      360

cugaagaagu ccgacgacga caaccuccag aaggacuuca agagcgccaa ggacacgauc      420

aagaagcaga ucuccgagua caucaaggac agcgagaagu ucaagaaccu guucaaccag      480

aaccugaucg acgcgaagaa gggccaggag uccgaccuca uccuguggcu gaagcagagc      540

aaggacaacg ggaucgagcu guucaaggcc aacuccgaca ucaccgacau cgacgaggcc      600

cucgagauca ucaagagcuu caagggcugg accaccuacu ucaagggcuu ccacgagaac      660

cgcaagaacg uguacuccag caacgacauc cccacgucca ucaucuaccg gaucguggac      720

gacaaccugc ccaaguuccu cgagaacaag gccaaguacg agagccugaa ggacaaggcg      780

ccggaggcca ucaacuacga gcagaucaag aaggaccugg ccgaggagcu caccuucgac      840

aucgacuaca agaccuccga ggucaaccag cgcguguuca gccuggacga gguguucgag      900

aucgccaacu ucaacaacua ccugaaccag agcgggauca ccaaguucaa cacgaucauc      960

ggcgggaagu ucgucaacgg cgagaacacc aagcggaagg ggaucaacga guacaucaac     1020

cucuacuccc agcagaucaa cgacaagacc cugaagaagu acaagaugag cgugcuguuc     1080

aagcagaucc ucuccgacac cgagagcaag uccuucguga ucgacaagcu ggaggacgac     1140

agcgacgucg ugacgaccau gcaguccuuc uacgagcaga ucgccgcguu caagaccgug     1200

gaggagaagu ccaucaagga gacccugucc cuccuguucg acgaccugaa ggcccagaag     1260

cucgaccuga gcaagaucua cuucaagaac gacaaguccc ugacggaccu cagccagcag     1320

gucuucgacg acuacuccgu gaucggcacc gccgugcugg aguacaucac ccagcagauc     1380

gcccccaaga accuggacaa ccccagcaag aaggagcagg agcucaucgc caagaagacc     1440

gagaaggcga aguaccuguc ccuggagacg aucaagcucg cccuggagga guucaacaag     1500

caccgcgaca ucgacaagca gugccgguuc gaggagaucc uggccaacuu cgccgccauc     1560

cccaugaucu ucgacgagau cgcgcagaac aaggacaacc ucgcccagau cagcaucaag     1620

uaccagaacc agggcaagaa ggaccugcug caggccuccg ccgaggacga cgucaaggcc     1680

aucaaggacc uccuggacca gaccaacaac cugcugcaca agcucaagau cuuccacauc     1740

agccaguccg aggacaaggc gaacauccug gacaaggacg agcacuucua ccugguguuc     1800

gaggagugcu acuucgagcu cgccaacauc gugccgcugu acaacaagau ccgcaacuac     1860

aucacccaga agcccuacag cgacgagaag uucaagcuga acuucgagaa cagcacccuc     1920

gccaacgggu gggacaagaa caaggagccc gacaacacgg ccauccuguu caucaaggac     1980

gacaaguacu accugggcgu caugaacaag aagaacaaca agaucuucga cgacaaggcg     2040

aucaaggaga acaaggggga gggcuacaag aagaucgugu acaagcuccu gcccggcgcc     2100

aacaagaugc ugccgaaggu guucuucucc gccaagagca ucaaguucua caaccccucc     2160

gaggacaucc uccggauccg caaccacagc acccacacca agaacgggag cccccagaag     2220

ggcuacgaga aguucgaguu caacaucgag gacugccgga aguucaucga cuucuacaag     2280

cagagcaucu ccaagcaccc cgaguggaag gacuucgggu uccgcuucag cgacacccag     2340

cgguacaacu ccaucgacga guucuaccgc gaggucgaga accagggcua caagcugacg     2400

uucgagaaca ucagcgaguc cuacaucgac agcgugguca accaggggaa gcuguaccuc     2460

uuccagaucu acaacaagga cuucuccgcc uacagcaagg gccggccgaa ccugcacacc     2520

cuguacugga aggcccucuu cgacgagcgc aaccugcagg acguggugua caagcugaac     2580

ggcgaggcgg agcuguucua ccggaagcag uccaucccca agaagaucac ccaccccgcc     2640

aaggaggcca ucgccaacaa gaacaaggac aaccccaaga aggagagcgu cuucgaguac     2700

gaccucauca aggacaagcg cuucaccgag gacaaguucu ucuuccacug cccgaucacg     2760

aucaacuuca aguccagcgg ggccaacaag uucaacgacg agaucaaccu gcuccugaag     2820

gagaaggcga acgacgugca cauccugagc aucgaccggg gcgagcgcca ccucgccuac     2880

uacacccugg uggacgggaa gggcaacauc aucaagcagg acaccuucaa caucaucggg     2940

aacgaccgca ugaagaccaa cuaccacgac aagcuggccg ccaucgagaa ggaccgggac     3000

uccgcccgca aggacuggaa gaagaucaac aacaucaagg agaugaagga gggcuaccuc     3060

agccaggucg ugcacgagau cgcgaagcug gugaucgagu acaacgccau cgucguguuc     3120

gaggaccuga acuucggcuu caagcggggg cgcuucaagg uggagaagca ggucuaccag     3180

aagcucgaga agaugcugau cgagaagcug aacuaccucg uguucaagga caacgaguuc     3240

gacaagacgg gcggggugcu gcgggccuac cagcugaccg cccccuucga gaccuucaag     3300

aagaugggca agcagaccgg gaucaucuac uacguccccg cgggcuucac cuccaagauc     3360

ugccccguga cgggcuucgu gaaccagcuc uacccgaagu acgagagcgu cuccaagagc     3420

caggaguucu ucuccaaguu cgacaagauc ugcuacaacc uggacaaggg guacuucgag     3480

uucagcuucg acuacaagaa cuucggcgac aaggccgcca aggggaagug gaccaucgcc     3540

uccuucggca gccgccugau caacuuccgg aacuccgaca agaaccacaa cugggacacc     3600

cgcgaggugu accccaccaa ggagcucgag aagcugcuga aggacuacag caucgaguac     3660

ggccacgggg agugcaucaa ggccgcgauc ugcggcgagu ccgacaagaa guucuucgcc     3720

aagcucacga gcgugcugaa caccauccug cagaugcgga acuccaagac cgggaccgag     3780

cucgacuacc ugaucagccc cgucgccgac gugaacggca acuucuucga cucccgccag     3840

gcccccaaga acaugccgca ggacgccgac gcgaacgggg ccuaccacau cggccugaag     3900

ggccucaugc ugcuggggcg gaucaagaac aaccaggagg gcaagaagcu caaccuggug     3960

aucaagaacg aggaguacuu cgaguucguc cagaaccgca acaacaagcg gcccgccgcc     4020

acgaagaagg ccgggcaggc gaagaagaag aaguaaggac uagugcauca cauuuaaaag     4080

caucucagcc uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc     4140

uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau     4200

cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

agaauu                                                                4326


<210>  2426
<211>  4582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2426
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcggacaag aaguacagca      120

ucggccugga caucgggacc aacuccgugg gcugggccgu gaucaccgac gaguacaagg      180

uccccagcaa gaaguucaag gugcugggga acacggaccg gcacuccauc aagaagaacc      240

ugaucggcgc ccugcuguuc gacagcggcg agaccgccga ggccacccgc cugaagcgga      300

ccgcgcgccg gcgcuacacg cggcgcaaga accggaucug cuaccugcag gagaucuucu      360

ccaacgagau ggccaaggug gacgacagcu ucuuccaccg ccucgaggag uccuuccugg      420

ucgaggagga caagaagcac gagcggcacc ccaucuucgg gaacaucgug gacgaggugg      480

ccuaccacga gaaguacccc accaucuacc accuccgcaa gaagcucguc gacagcaccg      540

acaaggccga ccugcggcug aucuaccucg cgcuggccca caugaucaag uuccgcggcc      600

acuuccugau cgagggggac cucaacccgg acaacuccga cguggacaag cuguucaucc      660

agcuggugca gaccuacaac cagcuguucg aggagaaccc caucaacgcc agcggcgucg      720

acgccaaggc cauccugucc gcgcggcuga gcaagucccg ccggcucgag aaccugaucg      780

cccagcugcc cggggagaag aagaacggcc ucuucggcaa ccugaucgcc cugagccucg      840

ggcugacgcc caacuucaag uccaacuucg accuggccga ggacgccaag cuccagcuga      900

gcaaggacac cuacgacgac gaccuggaca accuccuggc gcagaucggc gaccaguacg      960

ccgaccuguu ccucgccgcc aagaaccugu ccgacgccau ccugcucagc gacauccugc     1020

gcgugaacac cgagaucacc aaggcgccgc uguccgccag caugaucaag cgguacgacg     1080

agcaccacca ggaccucacg cugcugaagg cccucgugcg ccagcagcug cccgagaagu     1140

acaaggagau cuucuucgac cagagcaaga acggguacgc cggcuacauc gacggcgggg     1200

ccucccagga ggaguucuac aaguucauca agcccauccu ggagaagaug gacggcaccg     1260

aggagcuccu ggucaagcug aaccgggagg accugcuccg caagcagcgc accuucgaca     1320

acgggagcau cccccaccag auccaccugg gcgagcugca cgcgauccuc cggcgccagg     1380

aggacuucua cccguuccug aaggacaacc gggagaagau cgagaagauc cugaccuucc     1440

gcauccccua cuacgugggg ccccucgccc ggggcaacuc ccgcuucgcc uggaugacgc     1500

gcaagagcga ggagaccauc acccccugga acuucgagga gguggucgac aagggcgccu     1560

ccgcgcagag cuucaucgag cggaugacca acuucgacaa gaaccugccg aacgagaagg     1620

ugcugcccaa gcacucccuc cuguacgagu acuucacggu guacaacgag cugaccaagg     1680

ucaaguacgu gaccgagggg augcggaagc ccgccuuccu cagcggcgag cagaagaagg     1740

ccaucgugga ccugcuguuc aagaccaacc gcaaggucac ggugaagcag cucaaggagg     1800

acuacuucaa gaagaucgag ugcuucgacu ccgucgagau cagcggggug gaggaccggu     1860

ucaacgccuc ccugggcacc uaccacgacc ugcucaagau caucaaggac aaggacuucc     1920

uggacaacga ggagaacgag gacauccugg aggacaucgu gcucacccug acccuguucg     1980

aggaccgcga gaugaucgag gagcggcuca agacguacgc ccaccuguuc gacgacaagg     2040

ucaugaagca gcugaagcgc cggcgcuaca ccgggugggg ccggcucagc cgcaagcuga     2100

ucaacggcau ccgggacaag caguccggga agaccauccu ggacuuccuc aagagcgacg     2160

gcuucgcgaa ccgcaacuuc augcagcuga uccacgacga cucccugacc uucaaggagg     2220

acauccagaa ggcccaggug agcgggcagg gcgacucccu ccacgagcac aucgccaacc     2280

uggccgggag ccccgccauc aagaagggca uccugcagac ggugaagguc guggacgagc     2340

ucgugaaggu caugggccgc cacaagccgg agaacaucgu gaucgagaug gcgcgggaga     2400

accagaccac ccagaagggg cagaagaacu cccgcgagcg gaugaagcgc aucgaggagg     2460

gcaucaagga gcuggggagc cagauccuga aggagcaccc cguggagaac acccagcucc     2520

agaacgagaa gcuguaccug uacuaccucc agaacggccg ggacauguac gucgaccagg     2580

agcuggacau caaccgccug agcgacuacg acguggacca caucgugccc caguccuucc     2640

ucaaggacga cagcaucgac aacaaggucc ugacgcgguc cgacaagaac cgcggcaaga     2700

gcgacaacgu gcccuccgag gaggugguca agaagaugaa gaacuacugg cggcagcugc     2760

ucaacgccaa gcugaucacc cagcgcaagu ucgacaaccu gaccaaggcc gagcgcgggg     2820

gccucagcga gcuggacaag gccggguuca ucaagcggca gcugguggag acccggcaga     2880

ucaccaagca cguggcccag auccucgacu cccgcaugaa cacgaaguac gacgagaacg     2940

acaagcugau ccgggagguc aaggugauca cccucaagag caagcucgug uccgacuucc     3000

gcaaggacuu ccaguucuac aagguccggg agaucaacaa cuaccaccac gcgcacgacg     3060

ccuaccugaa cgccguggug ggcaccgccc ugaucaagaa guacccgaag cucgagagcg     3120

aguucgucua cggggacuac aagguguacg acgugcgcaa gaugaucgcg aaguccgagc     3180

aggagaucgg caaggccacc gccaaguacu ucuucuacag caacaucaug aacuucuuca     3240

agacggagau cacccuggcc aacggcgaga uccggaagcg cccccugauc gagaccaacg     3300

gggagaccgg cgagaucguc ugggacaagg ggcgggacuu cgccacggug cgcaaggugc     3360

ucuccaugcc ccaggucaac aucgugaaga agaccgaggu gcagaccggc ggguucagca     3420

aggaguccau ccugcccaag cgcaacagcg acaagcugau cgcgcggaag aaggacuggg     3480

acccgaagaa guacggcggc uucgacuccc ccaccgucgc cuacagcgug cucguggucg     3540

ccaaggugga gaagggcaag agcaagaagc ugaaguccgu gaaggagcug cucgggauca     3600

cgaucaugga gcgcagcucc uucgagaaga accccaucga cuuccuggag gccaaggggu     3660

acaaggaggu caagaaggac cugaucauca agcucccgaa guacagccug uucgagcugg     3720

agaacggccg gaagcgcaug cucgccuccg cgggggagcu gcagaagggc aacgagcugg     3780

cccuccccag caaguacgug aacuuccugu accuggccuc ccacuacgag aagcucaagg     3840

gcagccccga ggacaacgag cagaagcagc uguucgugga gcagcacaag cacuaccugg     3900

acgagaucau cgagcagauc uccgaguuca gcaagcgggu cauccucgcc gacgccaacc     3960

uggacaaggu gcuguccgcg uacaacaagc accgcgacaa gcccauccgg gagcaggccg     4020

agaacaucau ccaccucuuc acccugacca accugggggc cccggccgcg uucaaguacu     4080

ucgacaccac gaucgaccgc aagcgguaca ccagcaccaa ggaggugcuc gacgccaccc     4140

ugauccacca guccaucacg ggccuguacg agacccgcau cgaccucagc cagcucgggg     4200

gcgacaagcg gcccgccgcc accaagaagg ccgggcaggc gaagaagaag aagggacuag     4260

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     4320

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     4380

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     4440

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     4560

ucuuuucaga gccaccagaa uu                                              4582


<210>  2427
<211>  5365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2427
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaacuuc aagauccugc      120

ccaucgccau cgaccucggc gugaagaaca ccgggguguu cagcgccuuc uaccagaagg      180

gcaccucccu ggagcggcug gacaacaaga acgggaaggu cuacgagcuc agcaaggacu      240

ccuacacgcu gcugaugaac aaccgcaccg cccggcgcca ccagcggcgc ggcaucgacc      300

ggaagcagcu cgugaagcgc cuguucaagc ugaucuggac cgagcagcuc aaccuggagu      360

gggacaagga cacccagcag gccaucagcu uccuguucaa ccggcgcggc uucuccuuca      420

ucacggacgg guacagcccc gaguaccuca acaucgugcc cgagcagguc aaggcgaucc      480

ugauggacau cuucgacgac uacaacggcg aggacgaccu ggacuccuac cucaagcugg      540

ccaccgagca ggagagcaag aucuccgaga ucuacaacaa gcugaugcag aagauccucg      600

aguucaagcu gaugaagcug ugcaccgaca ucaaggacga caaggugagc accaagacgc      660

ucaaggagau caccuccuac gaguucgagc ugcuggccga cuaccucgcc aacuacagcg      720

agucccugaa gacccagaag uucagcuaca ccgacaagca ggggaaccug aaggagcucu      780

ccuacuacca ccacgacaag uacaacaucc aggaguuccu gaagcggcac gcgacgauca      840

acgaccgcau ccuggacacc cuccugaccg acgaccugga caucuggaac uucaacuucg      900

agaaguucga cuucgacaag aacgaggaga agcuccagaa ccaggaggac aaggaccaca      960

uccaggccca ccuccaccac uucguguucg ccgucaacaa gaucaagagc gagauggccu     1020

ccggcgggcg gcaccgcagc caguacuucc aggagaucac caacgugcug gacgagaaca     1080

accaccagga gggcuaccug aagaacuucu gcgagaaccu gcacaacaag aaguacucca     1140

accugagcgu gaagaaccuc gucaaccuga ucggcaaccu gagcaaccuc gagcugaagc     1200

cgcugcggaa guacuucaac gacaagaucc acgccaaggc ggaccacugg gacgagcaga     1260

aguucacgga gaccuacugc cacuggaucc ucggggagug gcgcgugggc gugaaggacc     1320

aggacaagaa ggacggggcc aaguacuccu acaaggaccu gugcaacgag cugaagcaga     1380

aggucaccaa ggccggccuc guggacuucc ugcuggagcu ggaccccugc cggaccaucc     1440

cgcccuaccu cgacaacaac aaccgcaagc cgcccaagug ccagagccug auccugaacc     1500

ccaaguuccu cgacaaccag uaccccaacu ggcagcagua ccugcaggag cugaagaagc     1560

uccaguccau ccagaacuac cuggacagcu ucgagacgga ccugaaggug cucaagucca     1620

gcaaggacca gcccuacuuc gucgaguaca aguccagcaa ccagcagauc gccuccggcc     1680

agcgggacua caaggaccug gacgcccgca uccugcaguu caucuucgac cgggugaagg     1740

cgagcgacga gcuccugcug aacgagaucu acuuccaggc caagaagcuc aagcagaagg     1800

ccuccagcga gcuggagaag cuggagucca gcaagaagcu cgacgaggug aucgccaacu     1860

cccagcugag ccagauccug aagucccagc acaccaacgg gaucuucgag cagggcaccu     1920

uccuccaccu ggucugcaag uacuacaagc agcgccagcg cgcccgggac agccgccugu     1980

acaucaugcc cgaguaccgg uacgacaaga agcuccacaa guacaacaac accgggcgcu     2040

ucgacgacga caaccagcug cugacguacu gcaaccacaa gccccggcag aagcgcuacc     2100

agcuccugaa cgaccuggcg ggcgugcucc aggugucccc caacuuccug aaggacaaga     2160

ucgggagcga cgacgaccug uucaucagca aguggcucgu cgagcacauc cggggcuuca     2220

agaaggccug cgaggacucc cugaagaucc agaaggacaa ccgcggccug cucaaccaca     2280

agaucaacau cgcccggaac accaagggga agugcgagaa ggagaucuuc aaccugaucu     2340

gcaagaucga gggcagcgag gacaagaagg ggaacuacaa gcacggccug gccuacgagc     2400

ucggggugcu gcuguucggc gagccgaacg aggccuccaa gcccgaguuc gaccgcaaga     2460

ucaagaaguu caacagcauc uacuccuucg cgcagaucca gcagaucgcc uucgccgagc     2520

ggaagggcaa cgccaacacg ugcgcggucu gcagcgccga caacgcccac cgcaugcagc     2580

agaucaagau caccgagccc guggaggaca acaaggacaa gaucauccuc uccgccaagg     2640

cccagcggcu gcccgcgauc ccgacccgca ucguggacgg ggccgucaag aagauggcca     2700

ccauccuggc caagaacauc guggacgaca acuggcagaa caucaagcag guccucagcg     2760

ccaagcacca gcugcacauc cccaucauca ccgaguccaa cgcguucgag uucgagcccg     2820

cccuggccga cgugaagggc aagagccuca aggaccggcg caagaaggcc cuggagcgga     2880

ucucccccga gaacaucuuc aaggacaaga acaaccgcau caaggaguuc gccaagggga     2940

ucagcgcgua cuccggcgcc aaccugaccg acggggacuu cgacggcgcc aaggaggagc     3000

ucgaccacau caucccgcgg agccacaaga aguacggcac gcugaacgac gaggccaacc     3060

ugaucugcgu gacccgcggg gacaacaaga acaagggcaa ccgcaucuuc ugccuccggg     3120

accuggcgga caacuacaag cugaagcagu ucgagaccac cgacgaccuc gagaucgaga     3180

agaagaucgc cgacacgauc ugggacgcca acaagaagga cuucaaguuc gggaacuacc     3240

gcuccuucau caaccugacg ccccaggagc agaaggccuu ccggcacgcc cuguuccucg     3300

cggacgagaa ccccaucaag caggccguga uccgcgccau caacaaccgg aaccgcaccu     3360

ucgucaacgg cacccagcgg uacuucgccg aggugcuggc caacaacauc uaccugcgcg     3420

cgaagaagga gaaccucaac accgacaaga ucagcuucga cuacuucggc auccccacca     3480

ucgggaacgg ccgggggauc gccgagaucc gccagcugua cgagaaggug gacuccgaca     3540

uccaggccua cgccaagggc gacaagccgc aggccagcua cagccaccug aucgacgcga     3600

ugcucgccuu cugcaucgcc gccgacgagc accggaacga cggguccauc ggccuggaga     3660

ucgacaagaa cuacagccug uacccccucg acaagaacac cggcgagguc uucaccaagg     3720

acaucuucuc ccagaucaag aucaccgaca acgaguucag cgacaagaag cuggugcgca     3780

agaaggcgau cgagggguuc aacacgcacc ggcagaugac ccgcgacggc aucuacgccg     3840

agaacuaccu gcccauccuc auccacaagg agcugaacga ggugcggaag ggguacaccu     3900

ggaagaacuc cgaggagauc aagaucuuca agggcaagaa guacgacauc cagcagcuga     3960

acaaccucgu cuacugccug aaguucgugg acaagccgau cagcaucgac auccagaucu     4020

ccacccugga ggagcuccgc aacauccuga cgaccaacaa caucgccgcc accgccgagu     4080

acuacuacau caaccugaag acccagaagc uccacgagua cuacaucgag aacuacaaca     4140

cggcgcuggg guacaagaag uacagcaagg agauggaguu ccugcggucc cucgccuacc     4200

gcagcgagcg ggugaagauc aaguccaucg acgacgucaa gcagguccug gacaaggaca     4260

gcaacuucau caucggcaag aucacccugc ccuucaagaa ggaguggcag cgccucuacc     4320

gggaguggca gaacaccacc aucaaggacg acuacgaguu ccugaagucc uucuucaacg     4380

ucaagagcau cacgaagcug cacaagaagg ugcgcaagga cuucucccuc cccaucagca     4440

ccaacgaggg caaguuccug gugaagcgca agaccuggga caacaacuuc aucuaccaga     4500

uccugaacga cuccgacagc cgggccgacg ggaccaagcc cuucaucccg gccuucgaca     4560

ucuccaagaa cgagaucgug gaggccauca ucgacagcuu cacgagcaag aacaucuucu     4620

ggcuccccaa gaacaucgag cugcagaagg uggacaacaa gaacaucuuc gcgaucgaca     4680

ccuccaagug guucgaggug gagaccccca gcgaccugcg cgacaucggc aucgccacca     4740

uccaguacaa gaucgacaac aacucccggc ccaaggugcg cgucaagcug gacuacguga     4800

ucgacgacga cagcaagauc aacuacuuca ugaaccacuc ccuccucaag agccgguacc     4860

cggacaaggu gcuggagauc cugaagcagu ccacgaucau cgaguucgag agcuccgggu     4920

ucaacaagac caucaaggag augcugggca ugaagcuggc cgggaucuac aacgagacca     4980

gcaacaacaa gcgccccgcc gccaccaaga aggcgggcca ggccaagaag aagaagggac     5040

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     5100

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     5160

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     5220

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     5340

ggcucuuuuc agagccacca gaauu                                           5365


<210>  2428
<211>  3637
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2428
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaagcgg aacuacaucc      120

ugggccucga caucgggauc accagcgugg gcuacgggau caucgacuac gagacccgcg      180

acgugaucga cgccggcguc cggcuguuca aggaggccaa cguggagaac aacgagggcc      240

gccgguccaa gcgcggggcc cggcgccuga agcggcgccg gcgccaccgg auccagcgcg      300

ugaagaagcu ccuguucgac uacaaccugc ucacggacca cagcgagcug uccggcauca      360

accccuacga ggcccggguc aaggggcuga gccagaagcu cuccgaggag gaguucagcg      420

cggcccugcu gcaccucgcc aagcgccggg gcgugcacaa cgugaacgag gucgaggagg      480

acaccgggaa cgagcugucc accaaggagc agaucagccg caacuccaag gcccuggagg      540

agaaguacgu ggcggagcuc cagcuggagc ggcugaagaa ggacggcgag gugcgcggca      600

gcaucaaccg guucaagacc uccgacuacg ucaaggaggc caagcagcuc cugaaggugc      660

agaaggccua ccaccagcug gaccagagcu ucaucgacac guacaucgac cuccuggaga      720

cccgccgcac cuacuacgag gggcccggcg aggggucccc cuucggcugg aaggacauca      780

aggaguggua cgagaugcug augggccacu gcaccuacuu cccggaggag cuccggagcg      840

ugaaguacgc cuacaacgcc gaccuguaca acgcgcugaa cgaccucaac aaccugguca      900

ucacgcgcga cgagaacgag aagcuggagu acuacgagaa guuccagauc aucgagaacg      960

uguucaagca gaagaagaag cccacccuca agcagaucgc caaggagauc cuggugaacg     1020

aggaggacau caagggguac cgggucaccu ccaccggcaa gcccgaguuc acgaaccuga     1080

agguguacca cgacaucaag gacaucaccg cccgcaagga gaucaucgag aacgccgagc     1140

uccuggacca gaucgccaag auccugacca ucuaccagag cuccgaggac auccaggagg     1200

agcucaccaa ccugaacagc gagcugacgc aggaggagau cgagcagauc uccaaccuca     1260

agggguacac cggcacccac aaccugagcc ugaaggcgau caaccucauc cuggacgagc     1320

uguggcacac caacgacaac cagaucgcca ucuucaaccg gcucaagcug gugcccaaga     1380

aggucgaccu gagccagcag aaggagaucc cgacgacccu cguggacgac uucauccugu     1440

cccccguggu caagcgcagc uucauccagu ccaucaaggu gaucaacgcc aucaucaaga     1500

aguacgggcu gcccaacgac aucaucaucg agcuggcccg ggagaagaac agcaaggacg     1560

cccagaagau gaucaacgag augcagaagc gcaaccggca gaccaacgag cgcaucgagg     1620

agaucauccg gaccacgggc aaggagaacg cgaaguaccu caucgagaag aucaagcugc     1680

acgacaugca ggagggcaag ugccuguacu cccucgaggc caucccccug gaggaccugc     1740

ucaacaaccc guucaacuac gaggucgacc acaucauccc ccgcagcgug uccuucgaca     1800

acagcuucaa caacaaggug cuggucaagc aggaggagaa cuccaagaag gggaaccgga     1860

cccccuucca guaccugagc uccagcgacu ccaagaucag cuacgagacc uucaagaagc     1920

acauccucaa ccuggccaag ggcaaggggc gcaucuccaa gaccaagaag gaguaccugc     1980

ucgaggagcg ggacaucaac cgcuucagcg ugcagaagga cuucaucaac cggaaccugg     2040

uggacacgcg cuacgccacc cggggccuga ugaaccuccu gcgcuccuac uuccgcguca     2100

acaaccugga cgugaagguc aagagcauca acgggggcuu caccuccuuc cuccggcgca     2160

aguggaaguu caagaaggag cggaacaagg gcuacaagca ccacgccgag gacgcgcuga     2220

ucaucgccaa cgccgacuuc aucuucaagg aguggaagaa gcuggacaag gccaagaagg     2280

ugauggagaa ccagauguuc gaggagaagc aggcggagag caugcccgag aucgagaccg     2340

agcaggagua caaggagauc uucaucacgc cgcaccagau caagcacauc aaggacuuca     2400

aggacuacaa guacagccac cgcguggaca agaagcccaa ccgggagcuc aucaacgaca     2460

cccuguacuc cacccgcaag gacgacaagg ggaacacccu gaucgugaac aaccucaacg     2520

gccuguacga caaggacaac gacaagcuga agaagcucau caacaagagc cccgagaagc     2580

ugcugaugua ccaccacgac ccccagacgu accagaagcu caagcugauc auggagcagu     2640

acggggacga gaagaacccg cuguacaagu acuacgagga gaccggcaac uaccucacca     2700

aguacuccaa gaaggacaac gggcccguca ucaagaagau caaguacuac ggcaacaagc     2760

ugaacgccca ccuggacauc accgacgacu accccaacag ccggaacaag guggugaagc     2820

ucucccugaa gcccuaccgc uucgacgucu accuggacaa cggcguguac aaguucguga     2880

ccgucaagaa ccucgacgug aucaagaagg agaacuacua cgaggugaac agcaagugcu     2940

acgaggaggc caagaagcug aagaagaucu ccaaccaggc cgaguucauc gccagcuucu     3000

acaacaacga ccugaucaag aucaacgggg agcucuaccg ggucaucggc gugaacaacg     3060

accugcugaa ccgcaucgag gugaacauga ucgacaucac guaccgggag uaccucgaga     3120

acaugaacga caagcgcccg ccccggauca ucaagaccau cgcguccaag acccagagca     3180

ucaagaagua cuccaccgac auccugggga accuguacga ggucaagagc aagaagcacc     3240

cccagaucau caagaagggc aagcgccccg ccgccacgaa gaaggccggc caggccaaga     3300

agaagaaggg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     3360

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     3420

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     3480

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     3600

ccccccccca aaggcucuuu ucagagccac cagaauu                              3637


<210>  2429
<211>  4705
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2429
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgcuguuc aacaagugca      120

ucaucaucag caucaaccuc gacuucucca acaaggagaa gugcaugacc aagcccuaca      180

gcaucggccu ggacaucggg accaacuccg ugggcugggc cgugaucacg gacaacuaca      240

agguccccag caagaagaug aaggugcugg ggaacaccuc caagaaguac aucaagaaga      300

accuccuggg cgugcugcug uucgacagcg gcaucaccgc cgaggggcgg cgccugaagc      360

ggaccgcccg ccggcgcuac acgcggcgcc ggaaccgcau ccuguaccuc caggagaucu      420

ucuccaccga gauggccacc cuggacgacg cguucuucca gcggcuggac gacagcuucc      480

ucguccccga cgacaagcgc gacuccaagu acccgaucuu cggcaaccug guggaggaga      540

agguguacca cgacgaguuc cccaccaucu accaccuccg gaaguaccuc gccgacagca      600

cgaagaaggc cgaccugcgc cuggucuacc ucgcccuggc gcacaugauc aaguaccggg      660

ggcacuuccu gaucgagggc gaguucaacu ccaagaacaa cgacauccag aagaacuucc      720

aggacuuccu cgacaccuac aacgccaucu ucgagagcga ccugucccug gagaacagca      780

agcagcucga ggagaucgug aaggacaaga ucuccaagcu ggagaagaag gaccgcaucc      840

ugaagcuguu ccccggggag aagaacagcg gcaucuucuc cgaguuccug aagcugaucg      900

ugggcaacca ggccgacuuc cggaagugcu ucaaccucga cgagaaggcc agccugcacu      960

ucuccaagga gagcuacgac gaggaccugg agacccuccu gggguacauc ggcgacgacu     1020

acagcgacgu cuuccugaag gccaagaagc ucuacgacgc gauccugcug uccggguucc     1080

ucaccgugac ggacaacgag accgaggcgc cccugagcuc cgccaugauc aagcgcuaca     1140

acgagcacaa ggaggaccug gcccuccuga aggaguacau ccggaacauc agccugaaga     1200

ccuacaacga gguguucaag gacgacacca agaacggcua cgccggcuac aucgacggga     1260

agacgaacca ggaggacuuc uacgucuacc ucaagaaccu gcuggccgag uucgagggcg     1320

ccgacuacuu ccucgagaag aucgaccgcg aggacuuccu gcggaagcag cgcaccuucg     1380

acaacggguc caucccguac cagauccacc uccaggagau gcgcgccauc cuggacaagc     1440

aggccaaguu cuaccccuuc cucgccaaga acaaggagcg gaucgagaag auccugaccu     1500

uccgcauccc cuacuacgug ggcccccugg cgcgggggaa cagcgacuuc gccuggucca     1560

uccgcaagcg gaacgagaag aucaccccgu ggaacuucga ggacgugauc gacaaggaga     1620

gcuccgccga ggccuucauc aaccgcauga cgagcuucga ccucuaccug cccgaggaga     1680

agguccugcc caagcacucc cuccuguacg agaccuucaa cguguacaac gagcugacca     1740

aggugcgguu caucgccgag agcaugcgcg acuaccaguu ccucgacucc aagcagaaga     1800

aggacaucgu ccggcuguac uucaaggaca agcgcaaggu gaccgacaag gacaucaucg     1860

aguaccugca cgcgaucuac ggcuacgacg gcaucgagcu caaggggauc gagaagcagu     1920

ucaacagcuc ccugagcacg uaccacgacc ugcucaacau caucaacgac aaggaguucc     1980

uggacgacuc cagcaacgag gccaucaucg aggagaucau ccacacccug accaucuucg     2040

aggaccggga gaugaucaag cagcgccucu ccaaguucga gaacaucuuc gacaagagcg     2100

ugcugaagaa gcugagccgg cgccacuaca ccggcugggg gaagcucucc gccaagcuga     2160

ucaacggcau ccgggacgag aaguccggga acacgauccu ggacuaccuc aucgacgacg     2220

gcaucagcaa ccgcaacuuc augcagcuga uccacgacga cgcccugagc uucaagaaga     2280

agauccagaa ggcgcagauc aucggcgacg aggacaaggg gaacaucaag gaggucguga     2340

agucccuccc cggcagcccg gccaucaaga aggggauccu gcaguccauc aagaucgucg     2400

acgagcuggu gaaggugaug ggcgggcgga agcccgagag caucgucgug gagauggccc     2460

gcgagaacca guacaccaac cagggcaagu ccaacagcca gcagcggcuc aagcgccugg     2520

agaagucccu gaaggagcuc ggcagcaaga uccugaagga gaacaucccc gccaagcugu     2580

ccaagaucga caacaacgcc cuccagaacg accgccugua ccuguacuac cuccagaacg     2640

ggaaggacau guacaccggc gacgaccugg acaucgaccg gcugagcaac uacgacaucg     2700

accacaucau cccccaggcg uuccucaagg acaacuccau cgacaacaag gugcugguca     2760

gcuccgccag caaccgcggg aaguccgacg acuucccgag ccuggaggug gugaagaagc     2820

ggaagaccuu cugguaccag cuccucaaga gcaagcugau cucccagcgc aaguucgaca     2880

accugacgaa ggccgagcgg ggcggccugc ugcccgagga caaggccggg uucauccagc     2940

gccagcucgu cgagacccgg cagaucacca agcacguggc ccgccugcug gacgagaagu     3000

ucaacaacaa gaaggacgag aacaaccggg cggugcgcac cgucaagauc aucacgcuca     3060

agagcacccu ggugucccag uuccggaagg acuucgagcu guacaaggug cgcgagauca     3120

acgacuucca ccacgcccac gacgccuacc ucaacgccgu caucgccagc gcgcugcuga     3180

agaaguaccc caagcucgag cccgaguucg uguacggcga cuacccgaag uacaacuccu     3240

uccgggagcg caagagcgcc accgagaagg uguacuucua cuccaacauc augaacaucu     3300

ucaagaaguc caucagccug gccgacgggc gggucaucga gcgcccccug aucgagguga     3360

acgaggagac cggcgagagc guguggaaca aggaguccga ccucgccacc guccggcgcg     3420

ugcugagcua cccccaggug aacgucguga agaaggugga ggagcagaac cacgggcugg     3480

accggggcaa gcccaagggc cucuucaacg cgaaccuguc cagcaagccg aagcccaacu     3540

ccaacgagaa ccuggucggg gccaaggagu accucgaccc caagaaguac ggcggguacg     3600

ccggcaucag caacuccuuc gccgugcugg ugaaggggac gaucgagaag ggcgccaaga     3660

agaagaucac caacguccug gaguuccagg gcaucagcau ccucgaccgc aucaacuacc     3720

ggaaggacaa gcugaacuuc cugcucgaga agggguacaa ggacaucgag cugaucaucg     3780

agcugccgaa guacagccuc uucgagcugu ccgacggcag ccgccgcaug cuggcgucca     3840

uccucagcac caacaacaag cgcggggaga uccacaaggg caaccagauc uuccuguccc     3900

agaaguucgu gaagcugcuc uaccacgcca agcggaucag caacaccauc aacgagaacc     3960

accggaagua cguggagaac cacaagaagg aguucgagga gcuguucuac uacauccugg     4020

aguucaacga gaacuacguc ggggccaaga agaacggcaa gcuccugaac uccgccuucc     4080

agagcuggca gaaccacucc aucgacgagc ugugcagcuc cuucaucggc cccacgggga     4140

gcgagcgcaa gggccucuuc gagcugaccu cccgggggag cgccgcggac uucgaguucc     4200

ugggcgugaa gaucccccgc uaccgggacu acacccccuc cagccuccug aaggacgcca     4260

cccugaucca ccaguccgug acggggcugu acgagacccg caucgaccuc gccaagcugg     4320

gcgagggcaa gcggccggcc gccaccaaga aggcggggca ggccaagaag aagaagggac     4380

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     4440

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     4500

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     4560

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     4680

ggcucuuuuc agagccacca gaauu                                           4705


<210>  2430
<211>  3781
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2430
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgugguac gccagccuga      120

uguccgccca ccaccuccgg gugggcaucg acguggggac ccacagcguc ggccuggcca      180

cccugcgcgu ggacgaccac gggacgccca ucgagcuccu guccgcccug agccacaucc      240

acgacuccgg cgugggcaag gaggggaaga aggaccacga cacccggaag aagcucagcg      300

gcaucgcgcg ccgggcccgc cggcugcugc accaccgccg gacccagcuc cagcagcugg      360

acgagguccu gcgcgaccuc ggguucccca uccccacccc gggcgaguuc cuggaccuga      420

acgagcagac ggaccccuac cggguguggc gcgugcgggc ccgccucguc gaggagaagc      480

ugcccgagga gcugcggggg cccgccaucu ccauggcggu gcgccacauc gcccggcacc      540

gcggcuggcg gaacccguac agcaaggugg agucccuccu gagccccgcc gaggaguccc      600

ccuucaugaa ggcccugcgc gagcggaucc ucgccaccac cggcgagguc cuggacgacg      660

ggaucacccc cggccaggcg auggcccagg uggcccugac gcacaacauc agcaugcgcg      720

ggccggaggg cauccucggc aagcugcacc aguccgacaa cgccaacgag auccgcaaga      780

ucugcgcccg gcagggggug agccccgacg ucugcaagca gcugcuccgc gcgguguuca      840

aggccgacag cccccggggc uccgccgugu cccgcgucgc ccccgacccg cugcccgggc      900

agggcagcuu ccggcgcgcc cccaagugcg accccgaguu ccagcgguuc cgcaucaucu      960

ccaucguggc gaaccugcgg aucagcgaga ccaaggggga gaaccgcccg cucaccgccg     1020

acgagcggcg ccacgugguc accuuccuga cggaggacag ccaggccgac cugaccuggg     1080

uggacguggc cgagaagcuc ggcguccacc ggcgcgaccu gcggggcacc gccgugcaca     1140

ccgacgacgg ggagcgcucc gcggcccggc cgcccaucga cgccacggac cgcaucaugc     1200

ggcagaccaa gaucagcucc cugaagaccu ggugggagga ggccgacagc gagcagcgcg     1260

gcgcgaugau ccgcuaccuc uacgaggacc ccaccgacuc cgagugcgcc gagaucaucg     1320

ccgagcugcc cgaggaggac caggccaagc uggacagccu ccaccucccc gccgggcggg     1380

cggccuacuc ccgcgagagc cugacggccc uguccgacca caugcuggcc accaccgacg     1440

accugcacga ggcccggaag cgccucuucg gcgucgacga cagcugggcg ccgcccgccg     1500

aggccaucaa cgcccccgug gggaaccccu ccguggaccg gacccugaag aucgucggcc     1560

gcuaccugag cgccguggag uccauguggg gcacgcccga ggugauccac gucgagcacg     1620

ugcgggacgg guucaccagc gagcgcaugg cggacgagcg ggacaaggcc aaccgccggc     1680

gcuacaacga caaccaggag gccaugaaga agauccagcg ggacuacggc aaggaggggu     1740

acaucucccg cggcgacauc gugcggcucg acgcccugga gcugcagggg ugcgcgugcc     1800

uguacugcgg caccaccauc ggcuaccaca cgugccagcu cgaccacauc gucccgcagg     1860

ccgggcccgg cagcaacaac cgccggggga accugguggc cgugugcgag cgcugcaacc     1920

gguccaagag caacaccccc uucgccgucu gggcccagaa gugcggcauc ccccacgugg     1980

gcgugaagga ggcgaucggg cgcguccggg gcuggcgcaa gcagaccccg aacaccucca     2040

gcgaggaccu gacgcgccuc aagaaggagg ugaucgcccg gcugcgccgg acccaggagg     2100

accccgagau cgacgagcgc agcauggagu ccguggccug gauggccaac gagcugcacc     2160

accggaucgc cgcggccuac cccgagacca ccgucauggu guaccgcggg agcaucacgg     2220

ccgccgcccg gaaggcggcc ggcaucgacu cccgcaucaa ccucaucggg gagaagggcc     2280

ggaaggaccg caucgaccgg cgccaccacg ccguggacgc cagcgucgug gcgcugaugg     2340

aggccuccgu ggccaagacc cuggccgagc ggagcucccu ccgcggcgag cagcggcuga     2400

ccgggaagga gcagaccugg aagcaguaca ccggcagcac ggucggggcc cgcgagcacu     2460

ucgagaugug gcggggccac augcugcacc ucaccgagcu guucaacgag cgccuggcgg     2520

aggacaaggu guacgugacc cagaacaucc ggcuccgccu guccgacggg aacgcccaca     2580

ccgucaaccc cagcaagcug gugucccacc gccucggcga cggccugacg gugcagcaga     2640

ucgaccgggc cugcaccccg gcccuguggu gcgcccucac ccgcgagaag gacuucgacg     2700

agaagaacgg gcugcccgcg cgggaggacc gcgccauccg gguccacggc cacgagauca     2760

agagcuccga cuacauccag guguucagca agcgcaagaa gaccgacucc gaccgggacg     2820

agacgcccuu cggggccauc gccgugcgcg gcggguucgu cgagaucggc cccagcaucc     2880

accacgcccg gaucuaccgc guggagggca agaagccggu guacgcgaug cugcgggucu     2940

ucacccacga ccuccugucc cagcgccacg gggaccuguu cagcgccgug aucccgcccc     3000

agagcaucuc caugcggugc gccgagccca agcuccgcaa ggccaucacc accggcaacg     3060

ccacguaccu ggggugggug gucgugggcg acgagcugga gaucaacgug gacagcuuca     3120

ccaaguacgc gaucgggcgg uuccucgagg acuuccccaa caccacccgc uggcggaucu     3180

gcggcuacga cacgaacucc aagcugaccc ugaagcccau cguccucgcc gccgagggcc     3240

uggagaaccc cagcuccgcc gugaacgaga ucguggagcu gaaggggugg cgcgucgcga     3300

ucaacgugcu caccaaggug caccccaccg ucgugcggcg cgacgcccug ggccggccgc     3360

gcuacagcuc ccgcagcaac cugcccacgu ccuggaccau cgagaagcgg cccgccgcca     3420

ccaagaaggc cgggcaggcg aagaagaaga agggacuagu gcaucacauu uaaaagcauc     3480

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     3540

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     3600

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     3720

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     3780

u                                                                     3781


<210>  2431
<211>  4642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2431
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaccaag cccuacagca      120

ucggccugga caucgggacc aacuccgugg gcugggccgu gacgaccgac aacuacaagg      180

uccccagcaa gaagaugaag gugcucggga acaccuccaa gaaguacauc aagaagaacc      240

ugcugggcgu gcuccuguuc gacagcggca ucaccgccga ggggcggcgc cugaagcgga      300

cggcccgccg gcgcuacacc cggcgccgga accgcauccu cuaccugcag gagaucuucu      360

ccaccgagau ggccacccug gacgacgcgu ucuuccagcg gcucgacgac agcuuccugg      420

uccccgacga caagcgcgac uccaaguacc cgaucuucgg caaccuggug gaggagaagg      480

ccuaccacga cgaguucccc acgaucuacc accuccggaa guaccuggcc gacagcacca      540

agaaggccga ccugcgccuc guguaccugg cgcuggccca caugaucaag uaccgggggc      600

acuuccucau cgagggcgag uucaacucca agaacaacga cauccagaag aacuuccagg      660

acuuccugga caccuacaac gccaucuucg agagcgaccu gucccucgag aacagcaagc      720

agcuggagga gaucgucaag gacaagaucu ccaagcugga gaagaaggac cgcauccuca      780

agcuguuccc cggggagaag aacagcggca ucuucuccga guuccugaag cucaucgugg      840

gcaaccaggc cgacuuccgg aagugcuuca accuggacga gaaggccagc cugcacuucu      900

ccaaggagag cuacgacgag gaccucgaga cccugcuggg guacaucggc gacgacuacu      960

ccgacguguu ccucaaggcg aagaagcugu acgacgccau ccugcucagc ggguuccuga     1020

cggucaccga caacgagacc gaggcgcccc ugagcuccgc caugaucaag cgcuacaacg     1080

agcacaagga ggaccucgcc cugcugaagg aguacauccg gaacaucagc cucaagaccu     1140

acaacgaggu guucaaggac gacacgaaga acggcuacgc cggcuacauc gacgggaaga     1200

ccaaccagga ggacuucuac guguaccuga agaagcugcu cgccgaguuc gagggcgccg     1260

acuacuuccu ggagaagauc gaccgcgagg acuuccugcg gaagcagcgc accuucgaca     1320

acggguccau cccguaccag auccaccucc aggagaugcg cgccauccug gacaagcagg     1380

ccaaguucua ccccuuccug gcgaagaaca aggagcggau cgagaagauc cugaccuucc     1440

gcauccccua cuacgucggc ccccucgccc gggggaacag cgacuucgcc ugguccaucc     1500

gcaagcggaa cgagaagauc acgccgugga acuucgagga cgugaucgac aaggagagcu     1560

ccgccgaggc cuucaucaac cgcaugacca gcuucgaccu guaccugccc gaggagaagg     1620

ugcuccccaa gcacucccug cuguacgaga ccuucaacgu cuacaacgag cucaccaagg     1680

ugcgguucau cgcggagagc augcgcgacu accaguuccu ggacuccaag cagaagaagg     1740

acaucgugcg gcuguacuuc aaggacaagc gcaaggucac ggacaaggac aucaucgagu     1800

accuccacgc caucuacggc uacgacggca ucgagcugaa ggggaucgag aagcaguuca     1860

acagcucccu gagcaccuac cacgaccucc ugaacaucau caacgacaag gaguuccugg     1920

acgacuccag caacgaggcc aucaucgagg agaucaucca cacccucacc aucuucgagg     1980

accgggagau gaucaagcag cgccugucca aguucgagaa caucuucgac aagagcgugc     2040

ugaagaagcu cucccggcgc cacuacacgg gcugggggaa gcugagcgcc aagcugauca     2100

acggcauccg ggacgagaag uccgggaaca ccauccucga cuaccugauc gacgacggca     2160

ucagcaaccg caacuucaug cagcugaucc acgacgacgc gcucagcuuc aagaagaaga     2220

uccagaaggc ccagaucauc ggcgacgagg acaaggggaa caucaaggag guggucaagu     2280

cccugcccgg cagcccggcc aucaagaagg ggauccugca guccaucaag aucguggacg     2340

agcucgucaa ggugaugggc gggcggaagc ccgagagcau cguggucgag auggcccgcg     2400

agaaccagua caccaaccag ggcaagucca acagccagca gcggcugaag cgccuggaga     2460

agucccucaa ggagcugggc agcaagaucc ugaaggagaa cauccccgcc aagcucucca     2520

agaucgacaa caacgcgcug cagaacgacc gccuguaccu cuacuaccug cagaacggga     2580

aggacaugua caccggcgac gaccuggaca ucgaccggcu cagcaacuac gacaucgacc     2640

acaucauccc ccaggccuuc cugaaggaca acuccaucga caacaaggug cuggugagcu     2700

ccgccagcaa ccgcgggaag uccgacgacg ucccgagccu cgagguggug aagaagcgga     2760

agacguucug guaccagcug cucaagagca agcugaucuc ccagcgcaag uucgacaacc     2820

ugaccaaggc cgagcggggc ggccugagcc ccgaggacaa ggccggguuc auccagcgcc     2880

agcucgucga gacccggcag aucaccaagc acguggcgcg ccugcuggac gagaaguuca     2940

acaacaagaa ggacgagaac aaccgggccg ugcgcacggu caagaucauc acccucaagu     3000

ccacccuggu gagccaguuc cggaaggacu ucgagcugua caaggugcgc gagaucaacg     3060

acuuccacca cgcccacgac gccuaccuca acgccgucgu ggcguccgcc cugcugaaga     3120

aguaccccaa gcucgagccc gaguucgugu acggcgacua cccgaaguac aacagcuucc     3180

gggagcgcaa guccgccacc gagaaggucu acuucuacag caacaucaug aacaucuuca     3240

agaaguccau cagccuggcc gacgggcggg ugaucgagcg cccccugauc gaggugaacg     3300

aggagaccgg cgaguccguc uggaacaagg agagcgaccu cgcgacggug cggcgcgugc     3360

uguccuaccc ccaggucaac guggugaaga aggucgagga gcagaaccac gggcuggacc     3420

ggggcaagcc caagggccuc uucaacgcca accugagcuc caagccgaag cccaacagca     3480

acgagaaccu ggugggggcc aaggaguacc ucgaccccaa gaaguacggc ggguacgccg     3540

gcaucuccaa cagcuucacc gugcugguca aggggaccau cgagaagggc gccaagaaga     3600

agaucaccaa cgugcuggag uuccagggca ucuccauccu cgaccgcauc aacuaccgga     3660

aggacaagcu gaacuuccug cucgagaagg gguacaagga caucgagcug aucaucgagc     3720

ugccgaagua cagccucuuc gagcugagcg acggcucccg ccgcaugcug gcgagcaucc     3780

ucuccacgaa caacaagcgc ggggagaucc acaagggcaa ccagaucuuc cugagccaga     3840

aguucgugaa gcugcucuac cacgccaagc ggaucuccaa caccaucaac gagaaccacc     3900

ggaaguacgu cgagaaccac aagaaggagu ucgaggagcu guucuacuac auccuggagu     3960

ucaacgagaa cuacgugggg gccaagaaga acggcaagcu ccugaacagc gccuuccagu     4020

ccuggcagaa ccacagcauc gacgagcugu gcuccagcuu caucggcccc accggguccg     4080

agcgcaaggg ccucuucgag cugaccagcc ggggguccgc cgcggacuuc gaguuccugg     4140

gcgugaagau cccccgcuac cgggacuaca cgcccagcuc ccuccugaag gacgccaccc     4200

ugauccacca gagcgucacc gggcucuacg agacccgcau cgaccuggcc aagcugggcg     4260

agggcaagcg gccggccgcc acgaagaagg cggggcaggc caagaagaag aagggacuag     4320

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     4380

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     4440

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     4500

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     4620

ucuuuucaga gccaccagaa uu                                              4642


<210>  2432
<211>  3724
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2432
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcggccgcc uucaagccca      120

acagcaucaa cuacauccug ggccucgaca ucgggaucgc cuccgugggc ugggccaugg      180

uggagaucga cgaggaggag aaccccaucc ggcugaucga ccuggggguc cgcguguucg      240

agcgggcgga ggugcccaag accggcgaca gccucgccau ggcccgccgg cuggcccgcu      300

ccguccggcg ccugacccgg cgccgggcgc accgccuccu gcggacgcgc cggcugcuca      360

agcgcgaggg cgugcugcag gccgccaacu ucgacgagaa cgggcugauc aagagccucc      420

cgaacacccc cuggcagcug cgggccgccg cgcuggaccg caagcucacg ccccuggagu      480

gguccgccgu gcugcuccac cugaucaagc accggggcua ccugagccag cgcaagaacg      540

agggggagac cgccgacaag gagcucggcg cccugcugaa gggggucgcc ggcaacgcgc      600

acgcccucca gaccggcgac uuccggaccc ccgccgagcu ggcccugaac aaguucgaga      660

aggaguccgg gcacauccgc aaccagcgca gcgacuacuc ccacaccuuc agccggaagg      720

accuccaggc cgagcugauc cuccuguucg agaagcagaa ggaguucggc aacccgcacg      780

uguccggggg ccugaaggag ggcaucgaga cccugcucau gacgcagcgc cccgcgcuga      840

gcggggacgc cgugcagaag augcugggcc acugcaccuu cgagcccgcc gagcccaagg      900

ccgccaagaa caccuacacc gcggagcggu ucaucuggcu cacgaagcug aacaaccugc      960

gcauccucga gcaggggucc gagcggccgc ugaccgacac cgagcgcgcc acccugaugg     1020

acgagcccua ccgcaagagc aagcucacgu acgcccaggc ccggaagcug cugggccucg     1080

aggacaccgc guucuucaag gggcugcggu acggcaagga caacgccgag gccuccaccc     1140

ugauggagau gaaggccuac cacgccauca gccgcgcgcu cgagaaggag ggccugaagg     1200

acaagaaguc cccgcugaac cugagccccg agcuccagga cgagaucggg accgccuucu     1260

cccuguucaa gacggacgag gacaucaccg gccggcugaa ggaccgcauc cagccggaga     1320

uccucgaggc ccugcugaag cacaucagcu ucgacaaguu cguccagauc agccucaagg     1380

cccugcggcg caucgugccc cugauggagc aggggaagcg guacgacgag gccugcgcgg     1440

agaucuacgg cgaccacuac gggaagaaga acaccgagga gaagaucuac cucccgccca     1500

uccccgccga cgagauccgc aaccccgugg uccugcgggc ccugucccag gcccgcaagg     1560

ugaucaacgg cgugguccgg cgcuacggca gccccgcccg cauccacauc gagaccgcgc     1620

gggagguggg gaaguccuuc aaggaccgca aggagaucga gaagcggcag gaggagaacc     1680

gcaaggaccg ggagaaggcc gccgccaagu uccgcgagua cuuccccaac uucgugggcg     1740

agcccaagag caaggacauc cucaagcugc ggcuguacga gcagcagcac gggaagugcc     1800

ucuacuccgg caaggagauc aaccuggggc gccugaacga gaagggcuac gucgagaucg     1860

accacgcgcu ccccuucagc cggacguggg acgacuccuu caacaacaag gugcuggugc     1920

ugggcagcga gaaccagaac aaggggaacc agacccccua cgaguacuuc aacggcaagg     1980

acaacucccg cgaguggcag gaguucaagg cccgggucga gaccagccgc uucccccggu     2040

ccaagaagca gcgcauccuc cugcagaagu ucgacgagga cggguucaag gagcggaacc     2100

ugaacgacac ccgcuacgug aaccgguucc ucugccaguu cgucgccgac cgcaugcggc     2160

ugacgggcaa gggcaagaag cgcguguucg ccagcaacgg gcagaucacc aaccugcucc     2220

ggggcuucug ggggcugcgc aaggugcgcg ccgagaacga ccggcaccac gcgcuggacg     2280

ccgucguggu ggccugcucc accgucgcca ugcagcagaa gaucacccgc uucgugcggu     2340

acaaggagau gaacgccuuc gacggcaaga cgaucgacaa ggagaccggg gaggugcucc     2400

accagaagac ccacuucccg cagcccuggg aguucuucgc gcaggagguc augauccgcg     2460

uguucggcaa gcccgacggc aagccggagu ucgaggaggc cgacacccug gagaagcugc     2520

ggacgcuccu ggccgagaag cugagcuccc gccccgaggc cgugcacgag uacgucacgc     2580

cccucuucgu gagccgggcc cccaaccgca agauguccgg gcagggccac auggagaccg     2640

ucaagagcgc gaagcggcug gacgaggggg uguccgugcu gcgcgugccg cucacccagc     2700

ugaagcugaa ggaccucgag aagaugguca accgggagcg cgagcccaag cuguacgagg     2760

cccugaaggc ccggcucgag gcccacaagg acgaccccgc gaaggccuuc gccgagcccu     2820

ucuacaagua cgacaaggcc ggcaaccgca cccagcaggu caaggccgug cggguggagc     2880

agguccagaa gaccggggug ugggugcgca accacaacgg caucgcggac aacgccacca     2940

uggugcgggu ggacgucuuc gagaagggcg acaaguacua ccuggugccg aucuacagcu     3000

ggcaggucgc caaggggauc cugcccgacc gcgccguggu gcagggcaag gacgaggagg     3060

acuggcagcu caucgacgac agcuucaacu ucaaguucuc ccugcacccc aacgaccugg     3120

uggaggucau caccaagaag gcccggaugu ucggguacuu cgcgagcugc caccgcggca     3180

ccgggaacau caacauccgc auccacgacc ucgaccacaa gaucggcaag aacggcaucc     3240

uggaggggau cggcgugaag acggcccugu ccuuccagaa guaccagauc gacgagcucg     3300

ggaaggagau ccggcccugc cgccugaaga agcggccgcc cgugcgcaag cggcccgccg     3360

ccaccaagaa ggccggccag gcgaagaaga agaagggacu agugcaucac auuuaaaagc     3420

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     3480

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     3540

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     3720

aauu                                                                  3724


<210>  2433
<211>  4480
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2433
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaagaag cccuacacca      120

ucggccugga caucgggacc aacagcgugg gcugggccgu gcucacggac caguacgacc      180

uggucaagcg gaagaugaag aucgccgggg acuccgagaa gaagcagauc aagaagaacu      240

ucuggggcgu gcgccuguuc gacgagggcc agaccgccgc cgaccggcgc auggcgcgga      300

ccgcccgccg gcgcaucgag cggcgccgga accgcaucag cuaccuccag gggaucuucg      360

ccgaggagau guccaagacc gacgccaacu ucuucugccg gcugagcgac uccuucuacg      420

uggacaacga gaagcgcaac agccggcacc ccuucuucgc gacgaucgag gaggaggucg      480

aguaccacaa gaacuacccc accaucuacc accuccgcga ggagcuggug aacuccagcg      540

agaaggccga ccugcggcug guguaccucg cccuggccca caucaucaag uaccgcggca      600

acuuccugau cgagggggcc cucgacaccc agaacaccuc cgucgacggc aucuacaagc      660

aguucaucca gacguacaac cagguguucg cgagcgggau cgaggacggc ucccugaaga      720

agcuggagga caacaaggac guggccaaga uccucgucga gaaggugacc cggaaggaga      780

agcuggagcg cauccugaag cucuacccgg gcgagaaguc cgccgggaug uucgcccagu      840

ucaucagccu gaucgugggc agcaagggga acuuccagaa gcccuucgac cugaucgaga      900

aguccgacau cgagugcgcc aaggacagcu acgaggagga ccucgagucc cugcuggcgc      960

ucaucggcga cgaguacgcc gagcuguucg ucgccgccaa gaacgccuac agcgcggugg     1020

ugcuguccag caucaucacc gucgccgaga ccgagacgaa cgccaagcuc agcgccucca     1080

ugaucgagcg guucgacacc cacgaggagg accugggcga gcugaaggcc uucaucaagc     1140

uccaccuccc caagcacuac gaggagaucu ucagcaacac cgagaagcac ggguacgcgg     1200

gcuacaucga cgggaagacc aagcaggccg acuucuacaa guacaugaag augacgcugg     1260

agaacaucga gggcgccgac uacuucaucg ccaagaucga gaaggagaac uuccugcgca     1320

agcagcgcac cuucgacaac ggggcgaucc cccaccagcu gcaccuggag gagcucgagg     1380

ccauccugca ccagcaggcc aaguacuacc cguuccugaa ggagaacuac gacaagauca     1440

agucccucgu gaccuuccgg auccccuacu ucgugggccc ccuggccaac ggccagagcg     1500

aguucgccug gcugacccgc aaggcggacg gggagauccg gcccuggaac aucgaggaga     1560

aggucgacuu cggcaagucc gccguggacu ucaucgagaa gaugacgaac aaggacaccu     1620

accucccgaa ggagaacgug cugcccaagc acagccugug cuaccagaag uaccucgucu     1680

acaacgagcu gaccaaggug cgcuacauca acgaccaggg gaagaccucc uacuucagcg     1740

gccaggagaa ggagcagauc uucaacgacc uguucaagca gaagcggaag gucaagaaga     1800

aggaccucga gcuguuccug cgcaacaugu cccacgugga gagccccacg aucgaggggc     1860

uggaggacuc cuucaacagc uccuacagca ccuaccacga ccuccugaag gugggcauca     1920

agcaggagau ccuggacaac cccgucaaca ccgagaugcu cgagaacauc gugaagaucc     1980

ugaccguguu cgaggacaag cggaugauca aggagcagcu gcagcaguuc uccgacgucc     2040

ucgacggcgu ggugcugaag aagcuggagc gccggcacua cacggggugg ggccgccuca     2100

gcgccaagcu gcugaugggg auccgggaca agcaguccca ccucaccauc cuggacuacc     2160

ugaugaacga cgacggccuc aaccgcaacc ugaugcagcu gaucaacgac agcaaccucu     2220

ccuucaagag caucaucgag aaggagcagg ucaccaccgc cgacaaggac auccagagca     2280

ucguggccga ccuggcgggg uccccggcca ucaagaaggg cauccugcag agccucaaga     2340

ucguggacga gcuggucucc gugaugggcu acccgcccca gacgaucgug gucgagaugg     2400

cccgggagaa ccagaccacc gggaagggca agaacaacag ccgcccccgg uacaaguccc     2460

uggagaaggc caucaaggag uucgggagcc agauccucaa ggagcacccc accgacaacc     2520

aggagcugcg caacaaccgg cuguaccucu acuaccugca gaacggcaag gacauguaca     2580

cgggccagga ccuggacauc cacaaccucu ccaacuacga caucgaccac aucgugcccc     2640

agagcuucau caccgacaac uccaucgaca accuggugcu gaccagcucc gccgggaacc     2700

gcgagaaggg cgacgacguc ccgccccucg agaucgugcg gaagcgcaag guguucuggg     2760

agaagcugua ccaggggaac cugaugagca agcgcaaguu cgacuaccuc accaaggcgg     2820

agcggggcgg gcugaccgag gccgacaagg cccgcuucau ccaccggcag cuggucgaga     2880

cgcgccagau caccaagaac guggccaaca uccuccacca gcgguucaac uacgagaagg     2940

acgaccacgg caacaccaug aagcaggucc gcaucgugac ccugaagucc gcgcugguga     3000

gccaguuccg gaagcaguuc cagcucuaca aggugcgcga cgucaacgac uaccaccacg     3060

cccacgacgc cuaccugaac ggcguggugg ccaacacgcu gcucaagguc uacccccagc     3120

uggagcccga guucguguac ggggacuacc accaguucga cugguucaag gccaacaagg     3180

cgaccgccaa gaagcaguuc uacaccaaca ucaugcuguu cuucgcccag aaggaccgga     3240

ucaucgacga gaacggcgag auccucuggg acaagaagua ccuggacacc gugaagaagg     3300

ucauguccua ccgccagaug aacaucguga agaagacgga gauccagaag ggggaguuca     3360

gcaaggccac caucaagccc aagggcaacu ccagcaagcu gaucccccgg aagaccaacu     3420

gggacccgau gaaguacggg ggccucgaca gccccaacau ggccuacgcg guggucaucg     3480

aguacgccaa gggcaagaac aagcuggugu ucgagaagaa gaucauccgc gugaccauca     3540

uggagcggaa ggccuucgag aaggacgaga aggccuuccu ggaggagcag ggguaccgcc     3600

agcccaaggu ccucgccaag cugccgaagu acacgcugua cgagugcgag gagggccggc     3660

gccggaugcu cgcguccgcc aacgaggccc agaaggggaa ccagcagguc cugcccaacc     3720

accuggugac ccuccugcac cacgccgcga acugcgaggu gagcgacggc aagucccugg     3780

acuacaucga gagcaaccgc gagauguucg ccgagcuccu ggcccacgug uccgaguucg     3840

ccaagcggua cacccuggcc gaggcgaacc ucaacaagau caaccagcug uucgagcaga     3900

acaaggaggg ggacaucaag gccaucgccc agagcuucgu ggaccugaug gccuucaacg     3960

ccaugggcgc gcccgccucc uucaaguucu ucgagaccac gaucgagcgc aagcgguaca     4020

acaaccucaa ggagcugcug aacagcacca ucaucuacca guccaucacc ggccucuacg     4080

agagccgcaa gcgccuggac gacaagcggc ccgccgccac caagaaggcc gggcaggcga     4140

agaagaagaa gggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     4200

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     4260

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     4320

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     4440

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           4480


<210>  2434
<211>  4513
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2434
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaagaag cccuacagca      120

ucggccugga caucgggacc aacuccgugg gcugggccgu ggucaccgac gacuacaagg      180

ugcccgccaa gaagaugaag gugcucggga acacggacaa gagccacauc gagaagaacc      240

ugcugggcgc ccuccuguuc gacuccggca acaccgccga ggaccggcgc cugaagcgga      300

ccgcgcgccg gcgcuacacc cggcgccgga accgcauccu cuaccugcag gagaucuuca      360

gcgaggagau ggggaagguc gacgacuccu ucuuccaccg gcuggaggac agcuuccucg      420

ugacggagga caagcgcggc gagcggcacc ccaucuucgg gaaccuggag gaggagguga      480

aguaccacga gaacuucccg accaucuacc accuccgcca guaccuggcc gacaaccccg      540

agaaggucga ccugcggcug guguaccucg cccuggccca caucaucaag uuccgcggcc      600

acuuccugau cgaggggaag uucgacaccc ggaacaacga cgugcagcgc cucuuccagg      660

aguuccuggc ggucuacgac aacaccuucg agaacuccag ccugcaggag cagaacgucc      720

agguggagga gauccucacg gacaagaucu ccaagagcgc caagaaggac cgggugcuga      780

agcuguuccc caacgagaag uccaacggcc gcuucgccga guuccucaag cugaucgugg      840

gcaaccaggc cgacuucaag aagcacuucg agcuggagga gaaggccccg cuccaguuca      900

gcaaggacac cuacgaggag gagcuggagg ugcugcucgc gcagaucggg gacaacuacg      960

ccgagcuguu ccuguccgcc aagaagcucu acgacagcau ccugcugucc ggcauccuca     1020

ccgucaccga cguggggacg aaggccccgc ugagcgccuc caugauccag cgguacaacg     1080

agcaccagau ggaccuggcg cagcucaagc aguucauccg ccagaagcug agcgacaagu     1140

acaacgaggu guucuccgac gucagcaagg acggcuacgc cggcuacauc gacgggaaga     1200

ccaaccagga ggccuucuac aaguaccuga agggccuccu gaacaagauc gaggggagcg     1260

gcuacuuccu ggacaagauc gagcgcgagg acuuccuccg gaagcagcgc accuucgaca     1320

acggguccau cccccaccag auccaccucc aggagaugcg ggccaucauc cgccggcagg     1380

ccgaguucua ccccuuccug gcggacaacc aggaccgcau cgagaagcug cugaccuucc     1440

ggauccccua cuacgugggc ccgcuggccc gcggcaagag cgacuucgcc uggcuguccc     1500

gcaagagcgc cgacaagauc acgcccugga acuucgacga gaucguggac aaggagucca     1560

gcgccgaggc guucaucaac cggaugacca acuacgaccu cuaccugccc aaccagaagg     1620

uccugcccaa gcacucccuc cuguacgaga aguucaccgu guacaacgag cugaccaagg     1680

ucaaguacaa gacggagcag gggaagaccg ccuucuucga cgccaacaug aagcaggaga     1740

ucuucgacgg cguguucaag guguaccgga aggucaccaa ggacaagcuc auggacuucc     1800

uggagaagga guucgacgag uuccgcaucg uggaccugac cgggcucgac aaggagaaca     1860

agguguucaa cgccagcuac ggcacguacc acgaccugug caagauccug gacaaggacu     1920

uccucgacaa cuccaagaac gagaagaucc uggaggacau cguccugacc cucacccugu     1980

ucgaggaccg ggagaugauc cgcaagcggc uggagaacua cagcgaccuc cugaccaagg     2040

agcaggucaa gaagcuggag cgccggcacu acacggggug gggccgccuc uccgcggagc     2100

ugauccacgg cauccggaac aaggagagcc gcaagaccau ccuggacuac cucaucgacg     2160

acgggaacuc caaccgcaac uucaugcagc ugaucaacga cgacgcccug agcuucaagg     2220

aggagaucgc caaggcccag gugaucggcg agaccgacaa ccucaaccag gugguguccg     2280

acaucgccgg gagcccggcg aucaagaagg gcauccugca gucccugaag aucguggacg     2340

agcucgucaa gaucaugggg caccagcccg agaacaucgu gguggagaug gcccgggaga     2400

accaguucac caaccagggc cgccggaaca gccagcagcg ccugaagggc cugacggacu     2460

ccaucaagga guucgggagc cagauccuca aggagcaccc cgucgagaac agccagcugc     2520

agaacgaccg gcuguuccuc uacuaccugc agaacggccg cgacauguac accggggagg     2580

agcuggacau cgacuaccuc ucccaguacg acaucgacca caucaucccc caggccuuca     2640

ucaaggacaa cagcaucgac aaccgggugc ugaccuccag caaggagaac cgcggcaagu     2700

ccgacgacgu gccgagcaag gacgucgugc ggaagaugaa guccuacugg agcaagcugc     2760

ucuccgccaa gcugaucacc cagcgcaagu ucgacaaccu gacgaaggcc gagcggggcg     2820

ggcucaccga cgacgacaag gcgggcuuca ucaagcgcca gcugguggag acccggcaga     2880

ucaccaagca cgucgcccgc auccuggacg agcgguucaa caccgagacg gacgagaaca     2940

acaagaagau ccgccaggug aagaucguga cccucaagag caaccugguc uccaacuucc     3000

ggaaggaguu cgagcuguac aaggugcgcg agaucaacga cuaccaccac gcccacgacg     3060

ccuaccucaa cgccgugauc gggaaggcgc ugcugggcgu cuacccccag cucgagcccg     3120

aguucgugua cggggacuac ccccacuucc acggccacaa ggagaacaag gccaccgcca     3180

agaaguucuu cuacagcaac aucaugaacu ucuucaagaa ggacgacgug cggaccgaca     3240

agaacggcga gaucaucugg aagaaggacg agcacaucuc caacaucaag aagguccuga     3300

gcuaccccca ggugaacauc gugaagaagg ucgaggagca gaccgggggc uucuccaagg     3360

agagcauccu gcccaagggg aacuccgaca agcucauccc ccgcaagacg aagaaguucu     3420

acugggacac caagaaguac ggcggguucg acagccccau cguggccuac agcauccugg     3480

ugaucgcgga caucgagaag ggcaagucca agaagcugaa gaccgucaag gcccucgugg     3540

gcgugacgau cauggagaag augaccuucg agcgggaccc ggucgccuuc cuggagcgca     3600

agggguaccg caacgugcag gaggagaaca ucaucaagcu gcccaaguac agccucuuca     3660

agcuggagaa cggccggaag cgccugcucg ccuccgcccg ggagcugcag aaggggaacg     3720

agaucgugcu gcccaaccac cucggcaccc ugcuguacca cgcgaagaac auccacaagg     3780

ucgacgagcc gaagcaccuc gacuacgugg acaagcacaa ggacgaguuc aaggagcugc     3840

uggacguggu cagcaacuuc uccaagaagu acacccucgc cgaggggaac cuggagaaga     3900

ucaaggagcu guacgcccag aacaacggcg aggaccucaa ggagcuggcc agcuccuuca     3960

ucaaccugcu cacguucacc gccaucggcg cgcccgccac cuucaaguuc uucgacaaga     4020

acaucgaccg caagcgguac accagcacga ccgagauccu gaacgccacc cugauccacc     4080

aguccaucac cgggcucuac gagacgcgca ucgaccugaa caagcugggc ggggacaagc     4140

ggcccgccgc caccaagaag gcgggccagg ccaagaagaa gaagggacua gugcaucaca     4200

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     4260

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     4320

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     4380

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     4500

agccaccaga auu                                                        4513


<210>  2435
<211>  3646
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2435
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgcagacc accaaccuga      120

gcuacauccu cggccuggac cuggggaucg ccuccguggg cugggccgug gucgagauca      180

acgagaacga ggaccccauc gggcucaucg acgugggcgu gcggaucuuc gagcgcgccg      240

agguccccaa gacgggcgag agccuggccc ugucccggcg ccucgcgcgg agcacccgcc      300

ggcugauccg ccggcgcgcc caccggcugc uccuggccaa gcgcuuccug aagcgggagg      360

ggauccucuc caccaucgac cuggagaagg gccugcccaa ccaggccugg gagcuccgcg      420

uggcggggcu ggagcggcgc cugagcgcca ucgagugggg cgccgugcuc cugcaccuga      480

ucaagcaccg gggguaccuc uccaagcgca agaacgagag ccagaccaac aacaaggagc      540

ugggcgcccu gcucuccggc gucgcccaga accaccagcu gcugcagagc gacgacuacc      600

ggacgccggc ggagcucgcc cugaagaagu ucgccaagga ggaggggcac auccgcaacc      660

agcggggcgc cuacacccac accuucaacc gccuggaccu ccuggccgag cugaaccucc      720

uguucgcgca gcagcaccag uucgggaacc cccacugcaa ggagcacauc cagcaguaca      780

ugaccgagcu gcucaugugg cagaagcccg cccuguccgg cgaggccauc cugaagaugc      840

ucggcaagug cacgcacgag aagaacgagu ucaaggccgc caagcacacc uacagcgcgg      900

agcgcuucgu guggcugacc aagcugaaca accuccggau ccuggaggac ggggccgagc      960

gcgcccugaa cgaggaggag cggcagcucc ugaucaacca ccccuacgag aaguccaagc     1020

ugaccuacgc ccaggugcgc aagcuccugg gccugagcga gcaggccauc uucaagcacc     1080

uccgguacuc caaggagaac gcggagagcg ccacguucau ggagcucaag gccuggcacg     1140

ccauccgcaa ggcgcuggag aaccaggggc ugaaggacac cuggcaggac cucgccaaga     1200

agccggaccu gcuggacgag aucggcaccg ccuucucccu cuacaagacc gacgaggaca     1260

uccagcagua ccugacgaac aaggucccca acagcgugau caacgcccug cucguguccc     1320

ugaacuucga caaguucauc gagcugagcc ucaagagccu gcggaagauc cugccccuca     1380

uggagcaggg gaagcgcuac gaccaggccu gccgggagau cuacggccac cacuacggcg     1440

aggcgaacca gaagaccucc cagcugcugc ccgccauccc ggcccaggag auccgcaacc     1500

ccgucgugcu ccggacccug agccaggccc gcaaggugau caacgccauc auccggcagu     1560

acgggucccc cgcgcgcguc cacaucgaga ccggccggga gcugggcaag agcuucaagg     1620

agcgccggga gauccagaag cagcaggagg acaaccgcac gaagcgggag uccgccgugc     1680

agaaguucaa ggagcuguuc agcgacuucu ccagcgagcc caaguccaag gacauccuga     1740

aguuccgccu guacgagcag cagcacggga agugccucua cagcgggaag gagaucaaca     1800

uccaccgccu gaacgagaag ggcuacgugg agaucgacca cgcccugccg uucucccgga     1860

ccugggacga cagcuucaac aacaaggucc ucgugcuggc cuccgagaac cagaacaagg     1920

gcaaccagac ccccuacgag uggcugcagg ggaagaucaa cagcgagcgc uggaagaacu     1980

ucguggcccu cguccugggc ucccagugca gcgcggccaa gaagcagcgg cugcucaccc     2040

aggugaucga cgacaacaag uucaucgacc gcaaccugaa cgacacgcgg uacaucgccc     2100

gcuuccuguc caacuacauc caggagaacc uccugcuggu cgggaagaac aagaagaacg     2160

uguucacccc caacggccag aucaccgccc uccugcggag ccgcuggggg cugaucaagg     2220

cgcgggagaa caacaaccgc caccacgccc ucgacgccau cguggucgcc ugcgccaccc     2280

ccuccaugca gcagaagauc acgcgguuca uccgcuucaa ggaggugcac ccguacaaga     2340

ucgagaaccg guacgagaug guggaccagg agagcggcga gaucaucagc ccccacuucc     2400

ccgagcccug ggcguacuuc cgccaggagg ucaacauccg gguguucgac aaccacccgg     2460

acaccgugcu gaaggagaug cugcccgacc gcccccaggc caaccaccag uucguccagc     2520

cccucuucgu gucccgggcc ccgacccgca agaugagcgg ccaggggcac auggagacca     2580

ucaaguccgc caagcggcug gccgagggca ucagcgugcu gcgcaucccc cucacgcagc     2640

ugaagcccaa ccugcucgag aacaugguca acaaggagcg ggagccggcg cuguacgccg     2700

ggcugaaggc ccgccucgcc gaguucaacc aggaccccgc caaggcguuc gccacccccu     2760

ucuacaagca gggcggccag caggucaagg ccauccgcgu ggagcagguc cagaaguccg     2820

gggugcuggu gcgggagaac aacggcgugg ccgacaacgc gagcaucgug cgcaccgacg     2880

uguucaucaa gaacaacaag uucuuccugg uccccaucua caccuggcag gucgccaagg     2940

ggauccuccc gaacaaggcc aucguggccc acaagaacga ggacgagugg gaggagaugg     3000

acgagggcgc caaguucaag uucucccugu uccccaacga ccugguggag cucaagacga     3060

agaaggagua cuucuucggg uacuacaucg gccuggaccg ggcgaccggc aacaucagcc     3120

ugaaggagca cgacggggag aucuccaagg gcaaggacgg gguguaccgc gugggcguca     3180

agcucgcccu gagcuucgag aaguaccagg uggacgagcu ggggaagaac cggcagaucu     3240

gccgccccca gcagcggcag cccgugcgca agcggccggc cgccaccaag aaggccggcc     3300

aggcgaagaa gaagaaggga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     3360

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     3420

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     3480

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     3600

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    3646


<210>  2436
<211>  3724
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2436
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcggccgcc uucaagccca      120

accccaucaa cuacauccug ggccucgaca ucgggaucgc cagcgugggc ugggccaugg      180

uggagaucga cgaggacgag aaccccaucu gccugaucga ccuggggguc cggguguucg      240

agcgcgcgga ggugccgaag accggcgacu cccucgccau ggcccggcgc cuggcccgga      300

gcguccgccg gcugacccgc cggcgcgcgc accggcuccu gcgcgcccgg cgccugcuca      360

agcgggaggg cgugcugcag gccgccgacu ucgacgagaa cgggcugauc aagucccucc      420

ccaacacgcc cuggcagcug cgcgccgcgg cccuggaccg gaagcucacg ccccuggagu      480

ggagcgccgu gcugcuccac cugaucaagc accgcggcua ccugucccag cggaagaacg      540

agggggagac cgccgacaag gagcucggcg cccugcugaa gggggucgcg gacaacgccc      600

acgcccucca gaccggcgac uuccgcaccc cggccgagcu ggcccugaac aaguucgaga      660

aggagagcgg ccacauccgg aaccagcgcg gggacuacuc ccacaccuuc agccgcaagg      720

accuccaggc ggagcugauc cuccucuucg agaagcagaa ggaguucggc aacccccacg      780

uguccggggg ccugaaggag ggcaucgaga cccugcucau gacccagcgg cccgcccuga      840

gcggggacgc cgugcagaag augcugggcc acugcacguu cgagcccgcc gagccgaagg      900

ccgcgaagaa caccuacacc gccgagcgcu ucaucuggcu caccaagcug aacaaccugc      960

ggauccucga gcaggggucc gagcgccccc ugacggacac cgagcgggcc acccugaugg     1020

acgagcccua ccgcaagagc aagcucaccu acgcccaggc gcggaagcug cugggccucg     1080

aggacacggc cuucuucaag gggcugcgcu acggcaagga caacgccgag gccuccaccc     1140

ugauggagau gaaggccuac cacgcgauca gccgggcccu cgagaaggag ggccugaagg     1200

acaagaaguc cccgcugaac cugagccccg agcuccagga cgagaucggg accgccuucu     1260

cccuguucaa gaccgacgag gacaucacgg gccgccugaa ggaccggauc cagcccgaga     1320

uccucgaggc ccugcugaag cacaucagcu ucgacaaguu cguccagauc ucccucaagg     1380

cccugcgccg gaucgugccc cugauggagc aggggaagcg cuacgacgag gcgugcgccg     1440

agaucuacgg cgaccacuac gggaagaaga acaccgagga gaagaucuac cugcccccga     1500

uccccgccga cgagauccgg aaccccgugg uccugcgcgc ccugagccag gcccggaagg     1560

ugaucaacgg cgugguccgc cgguacggca gccccgcgcg cauccacauc gagaccgccc     1620

gcgagguggg gaaguccuuc aaggaccgga aggagaucga gaagcgccag gaggagaacc     1680

ggaaggaccg cgagaaggcc gccgcgaagu uccgggagua cuucccgaac uucgugggcg     1740

agcccaagag caaggacauc cucaagcugc gccuguacga gcagcagcac gggaagugcc     1800

ucuacuccgg caaggagauc aaccuggggc ggcugaacga gaagggcuac gucgagaucg     1860

accacgcccu ccccuucagc cgcaccuggg acgacuccuu caacaacaag gugcuggugc     1920

ugggcagcga gaaccagaac aaggggaacc agacgcccua cgaguacuuc aacggcaagg     1980

acaacucccg ggaguggcag gaguucaagg cccgcgucga gaccagccgg uucccgcgcu     2040

ccaagaagca gcggauccuc cugcagaagu ucgacgagga cggguucaag gagcgcaacc     2100

ugaacgacac ccgguacgug aaccgcuucc ucugccaguu cgucgccgac cggaugcgcc     2160

ugaccggcaa gggcaagaag cggguguucg ccagcaacgg gcagaucacg aaccugcucc     2220

gcggcuucug ggggcugcgg aaggugcgcg cggagaacga ccgccaccac gcccuggacg     2280

ccgucguggu ggccugcucc accgucgcca ugcagcagaa gaucacccgg uucgugcgcu     2340

acaaggagau gaacgcguuc gacggcaaga ccaucgacaa ggagacgggg gaggugcucc     2400

accagaagac ccacuucccc cagcccuggg aguucuucgc ccaggagguc augauccggg     2460

uguucggcaa gccggacggc aagcccgagu ucgaggaggc cgacaccccc gagaagcugc     2520

gcacccugcu cgccgagaag cugagcuccc ggcccgaggc cgugcacgag uacgucacgc     2580

cgcuguucgu gagccgcgcg cccaaccgga agauguccgg gcagggccac auggagaccg     2640

ucaagagcgc caagcgccuc gacgaggggg uguccgugcu gcgggugccc cugacccagc     2700

ucaagcugaa ggaccuggag aagaugguca accgcgagcg ggagcccaag cucuacgagg     2760

cccugaaggc ccgccuggag gcgcacaagg acgacccggc caaggccuuc gccgagcccu     2820

ucuacaagua cgacaaggcc ggcaaccgga cccagcaggu caaggcggug cgcguggagc     2880

agguccagaa gaccggggug ugggugcgga accacaacgg caucgccgac aacgccacga     2940

uggugcgcgu ggacgucuuc gagaagggcg acaaguacua ccucgugccc aucuacagcu     3000

ggcaggucgc caaggggauc cugcccgacc gggccguggu gcagggcaag gacgaggagg     3060

acuggcagcu gaucgacgac uccuucaacu ucaaguucag ccuccacccg aacgaccugg     3120

uggaggucau caccaagaag gcgcgcaugu ucggguacuu cgccagcugc caccggggca     3180

ccgggaacau caacauccgc auccacgacc uggaccacaa gaucggcaag aacggcaucc     3240

ucgaggggau cggcgugaag accgcccugu ccuuccagaa guaccagauc gacgagcugg     3300

ggaaggagau ccgccccugc cggcucaaga agcgcccgcc cgugcggaag cgccccgccg     3360

ccacgaagaa ggcgggccag gccaagaaga agaagggacu agugcaucac auuuaaaagc     3420

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     3480

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     3540

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     3720

aauu                                                                  3724


<210>  2437
<211>  3730
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2437
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaaguac cacgugggca      120

ucgacguggg gaccuucagc gucggccugg ccgccaucga gguggacgac gccgggaugc      180

ccaucaagac ccugucccug gugagccaca uccacgacuc cggccuggac cccgacgaga      240

ucaagagcgc cgucacgcgg cucgcgucca gcggcaucgc ccgccggacc cgccggcugu      300

accgccggaa gcgccggcgc cugcagcagc ucgacaaguu cauccagcgg cagggguggc      360

ccgugaucga gcuggaggac uacuccgacc cgcuguaccc cuggaaggug cgcgccgagc      420

ucgccgcgag cuacaucgcc gacgagaagg agcggggcga gaagcugucc gucgcccugc      480

gccacaucgc ccggcaccgc ggguggcgga accccuacgc caaggugagc ucccucuacc      540

ugcccgacgg cccgagcgac gcguucaagg ccauccgcga ggagaucaag cgggccuccg      600

ggcagcccgu gcccgagacc gccaccgucg gccagauggu gacgcugugc gagcucggca      660

cccugaagcu gcgcggggag ggcggggugc ucagcgcccg gcugcagcag uccgacuacg      720

cgcgcgagau ccaggagauc ugccgcaugc aggagaucgg ccaggagcug uaccggaaga      780

ucaucgacgu cguguucgcc gccgagagcc ccaagggcuc cgccagcucc cgcgugggga      840

aggacccgcu ccagcccggc aagaaccggg cccugaaggc gagcgacgcc uuccagcgcu      900

accggaucgc cgcccugauc gggaaccucc gcguccgggu ggacggcgag aagcgcaucc      960

ugagcgugga ggagaagaac cuggucuucg accaccucgu gaaccugacc cccaagaagg     1020

agcccgagug ggugaccauc gccgagaucc uggggaucga ccggggccag cucaucggca     1080

cggcgaccau gaccgacgac ggggagcgcg ccggcgcccg gccgcccacc cacgacacga     1140

accgcuccau cgucaacagc cggaucgcgc cccuggugga cugguggaag accgccuccg     1200

cccuggagca gcacgccaug gucaaggccc ucagcaacgc cgagguggac gacuucgacu     1260

cccccgaggg ggcgaaggug caggccuucu ucgccgaccu ggacgacgac guccacgcca     1320

agcuggacag ccuccaccuc ccggugggcc gcgccgcgua cuccgaggac acccuggugc     1380

ggcucacccg ccggaugcug agcgacgggg ucgaccugua cacggcccgc cuccaggagu     1440

ucggcaucga gcccuccugg accccgccca cgccccggau cggcgagccc guggggaacc     1500

ccgccgugga ccgcguccug aagaccguga gccgcuggcu ggaguccgcc accaagaccu     1560

ggggcgcccc cgagcgggug aucaucgagc acguccgcga gggguucgug accgagaagc     1620

gggcgcgcga gauggacggc gacaugcggc gccgggccgc ccgcaacgcc aagcuguucc     1680

aggagaugca ggagaagcug aacgugcagg ggaagccgag ccgggcggac cuguggcgcu     1740

accaguccgu ccagcggcag aacugccagu gcgccuacug cggcagcccc aucaccuucu     1800

ccaacagcga gauggaccac aucgugcccc gcgccggcca gggguccacg aacacccggg     1860

agaaccucgu ggccgucugc caccgcugca accagagcaa gggcaacacc ccguucgcca     1920

ucugggcgaa gaacaccucc aucgaggggg ugagcgugaa ggaggccguc gagcggacgc     1980

gccacugggu gaccgacacc ggcaugcgga gcaccgacuu caagaaguuc acgaaggccg     2040

uggucgagcg cuuccagcgg gccaccaugg acgaggagau cgacgcccgc uccauggaga     2100

gcguggcgug gauggccaac gagcugcggu cccgcguggc ccagcacuuc gccagccacg     2160

gcaccaccgu ccgcguguac cggggguccc ugacggccga ggcgcgccgg gccagcggca     2220

ucuccgggaa gcugaaguuc uucgacggcg ugggcaagag ccgccucgac cggcgccacc     2280

acgccaucga cgccgcgguc aucgccuuca ccuccgacua cguggccgag acccuggccg     2340

ugcggagcaa ccugaagcag ucccaggccc accgccagga ggcgccccag uggcgggagu     2400

ucaccgggaa ggacgccgag caccgcgccg ccuggcgggu cuggugccag aagauggaga     2460

agcucagcgc ccugcugacc gaggaccucc gcgacgaccg gguggugguc auguccaacg     2520

ugcgccugcg gcugggcaac gggagcgcgc acaaggagac gaucggcaag cucuccaagg     2580

ugaagcugag cucccagcug agcgucagcg acaucgacaa ggccuccagc gaggcccucu     2640

ggugcgcccu gacccgcgag cccggguucg accccaagga gggccugccg gccaaccccg     2700

agcggcacau ccgcgugaac gggacccacg uguacgcggg cgacaacauc ggccucuucc     2760

ccgucuccgc cgggagcauc gcccugcggg gcggguacgc cgagcugggc uccagcuucc     2820

accacgcccg cguguacaag aucaccuccg ggaagaagcc cgcguucgcc augcuccgcg     2880

uguacacgau cgaccugcug ccguaccgga accaggaccu cuucagcguc gagcugaagc     2940

cccagaccau guccaugcgc caggccgaga agaagcugcg ggacgcccuc gcgaccggca     3000

acgccgagua ccugggcugg cugguggugg acgacgagcu cgucguggac accagcaaga     3060

ucgccacgga ccaggugaag gccgucgagg ccgagcuggg gaccauccgc cgguggcgcg     3120

uggacggcuu cuucuccccc agcaagcugc ggcuccgccc ccugcagaug uccaaggagg     3180

ggaucaagaa ggagagcgcg ccggagcugu ccaagaucau cgaccggccc ggcuggcucc     3240

ccgccgucaa caagcuguuc agcgacggga acgugaccgu gguccgccgg gacucccugg     3300

gccgcgugcg gcucgagagc accgcccacc uccccgugac guggaagguc cagaagcgcc     3360

cggccgccac caagaaggcg ggccaggcca agaagaagaa gggacuagug caucacauuu     3420

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     3480

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     3540

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     3600

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     3720

caccagaauu                                                            3730


<210>  2438
<211>  3430
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2438
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcggcccgg auccuggccu      120

ucgacaucgg caucagcucc aucggguggg ccuucagcga gaacgacgag cucaaggacu      180

gcggcgugcg caucuucacc aagguggaga accccaagac cggggagucc cuggcccugc      240

cccggcgccu cgcgcggagc gcccgcaagc ggcuggcccg ccggaaggcc cgccugaacc      300

accucaagca ccugaucgcg aacgaguuca agcugaacua cgaggacuac caguccuucg      360

acgagagccu cgccaaggcc uacaagggcu cccugaucag ccccuacgag cugcgguucc      420

gcgcccucaa cgagcugcug uccaagcagg acuucgcccg ggucauccuc cacaucgcga      480

agcgccgggg cuacgacgac aucaagaaca gcgacgacaa ggagaagggg gccauccuga      540

aggccaucaa gcagaacgag gagaagcugg ccaacuacca guccgugggc gaguaccucu      600

acaaggagua cuuccagaag uucaaggaga acagcaagga guucacgaac gugcgcaaca      660

agaaggaguc cuacgagcgg ugcaucgccc agagcuuccu gaaggacgag cugaagcuca      720

ucuucaagaa gcagcgcgag uucggguucu ccuucagcaa gaaguucgag gaggaggucc      780

uguccguggc guucuacaag cgggcccuga aggacuucag ccaccucgug ggcaacugcu      840

ccuucuucac cgacgagaag cgcgccccga agaacagccc ccuggccuuc auguucgucg      900

cccugacccg gaucaucaac cuccugaaca accugaagaa caccgagggg auccucuaca      960

cgaaggacga ccugaacgcg cugcucaacg aggugcugaa gaacggcacc cugaccuaca     1020

agcagaccaa gaagcuccug ggccugagcg acgacuacga guucaagggg gagaagggca     1080

cguacuucau cgaguucaag aaguacaagg aguucaucaa ggcccucggg gagcacaacc     1140

ugucccagga cgaccugaac gagaucgcca aggacaucac ccucaucaag gacgagauca     1200

agcugaagaa ggcccuggcc aaguacgacc ucaaccagaa ccagaucgac agccugucca     1260

agcuggaguu caaggaccac cucaacauca gcuucaaggc gcugaagcug gugacgcccc     1320

ugaugcucga gggcaagaag uacgacgagg ccugcaacga gcugaaccug aaggucgcca     1380

ucaacgagga caagaaggac uuccuccccg ccuucaacga gaccuacuac aaggacgagg     1440

ugaccaaccc ggugguccug cgcgcgauca aggaguaccg caaggugcug aacgcccucc     1500

ugaagaagua cggcaaggug cacaagauca acaucgagcu ggcccgggag gucgggaaga     1560

accacuccca gcgcgccaag aucgagaagg agcagaacga gaacuacaag gccaagaagg     1620

acgcggagcu cgagugcgag aagcugggcc ugaagaucaa cagcaagaac auccucaagc     1680

ugcggcuguu caaggagcag aaggaguucu gcgccuacuc cggggagaag aucaagauca     1740

gcgaccucca ggacgagaag augcuggaga ucgaccacau cuaccccuac ucccgcagcu     1800

ucgacgacuc cuacaugaac aaggugcugg uguucaccaa gcagaaccag gagaagcuca     1860

accagacccc cuucgaggcc uucggcaacg acagcgccaa guggcagaag aucgaggucc     1920

uggccaagaa ccugcccacc aagaagcaga agcggauccu cgacaagaac uacaaggaca     1980

aggagcagaa gaacuucaag gaccgcaacc ugaacgacac gcgguacauc gcgcgccugg     2040

ugcucaacua caccaaggac uaccuggacu uccugccgcu cuccgacgac gagaacacca     2100

agcugaacga cacccagaag gggagcaagg ugcacgucga ggccaagucc ggcaugcuga     2160

cgagcgcccu ccggcacacc uggggcuucu ccgccaagga ccgcaacaac caccuccacc     2220

acgccaucga cgcggugauc aucgccuacg ccaacaacag caucgucaag gccuucuccg     2280

acuucaagaa ggagcaggag agcaacagcg cggagcugua cgccaagaag aucuccgagc     2340

uggacuacaa gaacaagcgg aaguucuucg agcccuucag cggguuccgc cagaaggugc     2400

uggacaagau cgacgagauc uucgugucca agcccgagcg gaagaagccc agcggcgccc     2460

ugcacgagga gaccuuccgc aaggaggagg aguucuacca guccuacggg ggcaaggagg     2520

ggguccucaa ggcccuggag cugggcaaga uccggaaggu gaacggcaag aucgugaaga     2580

acggggacau guuccgcguc gacaucuuca agcacaagaa gaccaacaag uucuacgccg     2640

ugccgaucua cacgauggac uucgcgcuca aggugcugcc caacaaggcc gucgcccgga     2700

gcaagaaggg cgagaucaag gacuggaucc ugauggacga gaacuacgag uucugcuucu     2760

cccucuacaa ggacagccug auccugaucc agaccaagga caugcaggag cccgaguucg     2820

uguacuacaa cgccuucacc uccagcaccg ugucccucau cgucagcaag cacgacaaca     2880

aguucgagac gcuguccaag aaccagaaga uccuguucaa gaacgccaac gagaaggagg     2940

ugaucgccaa gagcaucggg auccagaacc ucaagguguu cgagaaguac aucgucuccg     3000

cgcugggcga ggugaccaag gccgaguucc gccagcggga ggacuucaag aagaagcgcc     3060

ccgccgccac caagaaggcg gggcaggcca agaagaagaa gggacuagug caucacauuu     3120

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     3180

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     3240

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     3300

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3360

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     3420

caccagaauu                                                            3430


<210>  2439
<211>  3841
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2439
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgagcgac cuggugcucg      120

gccuggacau cgggaucggc uccguggggg ucggcauccu gaacaaggug accggcgaga      180

ucauccacaa gaacagccgg aucuuccccg ccgcccaggc cgagaacaac cucgugcgcc      240

ggaccaaccg ccaggggcgg cgccuggccc ggcgcaagaa gcaccggcgc guccggcuga      300

accgccucuu cgaggagucc ggccugauca cggacuucac caagaucagc aucaaccuga      360

accccuacca gcuccgggug aaggggcuga ccgacgagcu guccaacgag gagcuguuca      420

ucgcgcugaa gaacauggug aagcaccgcg gcaucagcua ccuggacgac gccuccgacg      480

acgggaacag cuccgucggc gacuacgccc agaucgugaa ggagaacagc aagcagcucg      540

agaccaagac gcccggccag auccagcugg agcgguacca gaccuacggg cagcugcgcg      600

gcgacuucac cguggagaag gacgggaaga agcaccggcu caucaacguc uucccgaccu      660

ccgccuaccg cagcgaggcg cugcggaucc ugcagacgca gcaggaguuc aacccccaga      720

ucaccgacga guucaucaac cgcuaccucg agauccugac cggcaagcgg aaguacuacc      780

acggccccgg gaacgagaag ucccgcaccg acuacggccg cuaccggacg agcggggaga      840

cccuggacaa caucuucggc auccucaucg ggaagugcac cuucuacccc gacgaguucc      900

gcgccgccaa ggccuccuac accgcccagg aguucaaccu gcugaacgac cucaacaacc      960

ugacggugcc gaccgagacc aagaagcuga gcaaggagca gaagaaccag aucaucaacu     1020

acgugaagaa cgagaaggcg augggccccg ccaagcuguu caaguacauc gccaagcucc     1080

uguccugcga cgucgccgac aucaagggcu accggaucga caagagcggg aaggccgaga     1140

uccacaccuu cgaggcguac cgcaagauga agacgcucga gacccuggac aucgagcaga     1200

uggaccggga gacccuggac aagcucgccu acgugcugac ccugaacacg gagcgcgagg     1260

gcauccagga ggcccucgag cacgaguucg ccgacggguc cuucagccag aagcaggucg     1320

acgagcuggu gcaguuccgg aaggccaaca gcuccaucuu cggcaagggg uggcacaacu     1380

ucagcgugaa gcugaugaug gagcucaucc ccgagcugua cgagaccucc gaggagcaga     1440

ugaccauccu gacccgccuc ggcaagcaga agacgaccag cuccagcaac aagaccaagu     1500

acaucgacga gaagcugcug accgaggaga ucuacaaccc cguggucgcg aaguccgugc     1560

ggcaggccau caagaucgug aacgccgcca ucaaggagua cggcgacuuc gacaacaucg     1620

ucaucgagau ggcccgcgag acgaacgagg acgacgagaa gaaggcgauc cagaagaucc     1680

agaaggccaa caaggacgag aaggacgccg ccaugcucaa ggcggccaac caguacaacg     1740

ggaaggccga gcugccgcac agcguguucc acggccacaa gcagcuggcc accaagaucc     1800

ggcuguggca ccagcagggg gagcgcugcc ucuacaccgg caagaccauc uccauccacg     1860

accugaucaa caacagcaac caguucgagg ucgaccacau ccugccccuc uccaucacgu     1920

ucgacgacag ccuggccaac aaggugcugg uguacgcgac cgccaaccag gagaaggggc     1980

agcggacccc cuaccaggcc cucgacucca uggacgacgc cuggagcuuc cgcgagcuga     2040

aggccuucgu ccgggagucc aagacccuga gcaacaagaa gaaggaguac cuccugaccg     2100

aggaggacau cuccaaguuc gacgugcgca agaaguucau cgagcggaac cugguggaca     2160

cgcgcuacgc gagccggguc gugcucaacg cccugcagga gcacuuccgc gcccacaaga     2220

ucgacaccaa gguguccguc gugcgcggcc aguucaccag ccagcugcgg cgccacuggg     2280

gcaucgagaa gacccgggac acguaccacc accacgccgu ggacgcccuc aucaucgcgg     2340

ccagcuccca gcugaaccug uggaagaagc agaagaacac ccucgucagc uacuccgagg     2400

accagcugcu ggacaucgag accggggagc ucaucagcga cgacgaguac aaggaguccg     2460

uguucaaggc ccccuaccag cacuucgugg acacccucaa gagcaaggag uucgaggacu     2520

ccauccuguu cagcuaccag gucgacucca aguucaaccg caagaucagc gacgccacga     2580

ucuacgcgac ccggcaggcc aaggugggca aggacaaggc cgacgagacc uacgugcucg     2640

ggaagaucaa ggacaucuac acccaggacg gcuacgacgc cuucaugaag aucuacaaga     2700

aggacaaguc caaguuccug auguaccgcc acgacccgca gacguucgag aaggucaucg     2760

agcccauccu ggagaacuac cccaacaagc agaucaacga gaagggcaag gaggugcccu     2820

gcaacccguu ccucaaguac aaggaggagc acggguacau ccggaaguac agcaagaagg     2880

gcaacgggcc cgagaucaag ucccugaagu acuacgacag caagcugggc aaccacaucg     2940

acaucacccc caaggacucc aacaacaagg ugguccucca gagcgugucc cccuggcgcg     3000

ccgacgugua cuucaacaag accaccggga aguacgagau ccugggccug aaguacgcgg     3060

accuccaguu cgagaagggc acggggaccu acaagaucag ccaggagaag uacaacgaca     3120

ucaagaagaa ggagggcguc gacagcgacu ccgaguucaa guucacccug uacaagaacg     3180

accugcuccu ggugaaggac accgagacga aggagcagca gcuguuccgg uuccucagcc     3240

gcaccaugcc gaagcagaag cacuacgugg agcugaagcc cuacgacaag cagaaguucg     3300

agggcgggga ggcccugauc aagguccucg ggaacguggc caacuccggc cagugcaaga     3360

agggccuggg caagagcaac aucuccaucu acaaggugcg gaccgacguc cuggggaacc     3420

agcacaucau caagaacgag ggggacaagc ccaagcucga cuucaagcgc cccgccgcca     3480

ccaagaaggc gggccaggcc aagaagaaga agggacuagu gcaucacauu uaaaagcauc     3540

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     3600

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     3660

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     3780

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     3840

u                                                                     3841


<210>  2440
<211>  4399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2440
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgacccag uucgagggcu      120

ucaccaaccu guaccaggug agcaagacgc uccgguucga gcugaucccc caggggaaga      180

cccugaagca cauccaggag cagggcuuca ucgaggagga caaggcccgc aacgaccacu      240

acaaggagcu caagcccauc aucgaccgga ucuacaagac cuacgccgac cagugccugc      300

agcuggugca gcucgacugg gagaaccugu ccgccgccau cgacagcuac cgcaaggaga      360

agaccgagga gacgcggaac gcgcugaucg aggagcaggc caccuaccgc aacgccaucc      420

acgacuacuu caucgggcgg accgacaacc ucaccgacgc caucaacaag cgccacgcgg      480

agaucuacaa gggccuguuc aaggccgagc uguucaacgg caagguccuc aagcagcugg      540

ggacggugac caccaccgag cacgagaacg cccugcuccg guccuucgac aaguucacga      600

ccuacuucag cggcuucuac gagaaccgca agaacguguu cuccgccgag gacaucagca      660

ccgccauccc ccaccggauc guccaggaca acuucccgaa guucaaggag aacugccaca      720

ucuucacccg ccugaucacg gcggugcccu cccugcggga gcacuucgag aacgugaaga      780

aggccaucgg gaucuucguc agcaccucca ucgaggaggu guucagcuuc cccuucuaca      840

accagcuccu gacccagacc cagaucgacc uguacaacca gcuccugggc gggaucuccc      900

gcgaggccgg cacggagaag aucaagggcc ugaacgaggu gcucaaccug gccauccaga      960

agaacgacga gaccgcccac aucaucgcga gccugcccca ccgguucauc ccccucuuca     1020

agcagauccu guccgaccgc aacacccuga gcuucauccu cgaggaguuc aaguccgacg     1080

aggaggucau ccagagcuuc ugcaaguaca agacccugcu gcggaacgag aacgugcucg     1140

agacggccga ggcccuguuc aacgagcuga acuccaucga ccucacccac aucuucauca     1200

gccacaagaa gcuggagacc aucuccagcg cccugugcga ccacugggac acccuccgca     1260

acgcccugua cgagcggcgc aucagcgagc ugacggggaa gaucaccaag uccgcgaagg     1320

agaaggugca gcggagccuc aagcacgagg acaucaaccu gcaggagauc aucuccgccg     1380

ccggcaagga gcugagcgag gccuucaagc agaagaccuc cgagauccuc agccacgccc     1440

acgcggcccu ggaccagccg cugcccacca cgcucaagaa gcaggaggag aaggagaucc     1500

ugaaguccca gcuggacagc cugcucgggc uguaccaccu ccucgacugg uucgccgucg     1560

acgaguccaa cgagguggac cccgaguuca gcgcccgccu gaccggcauc aagcuggaga     1620

uggagccguc ccucagcuuc uacaacaagg cgcgcaacua cgccaccaag aagcccuacu     1680

ccguggagaa guucaagcug aacuuccaga ugcccacccu ggccagcggc ugggacguca     1740

acaaggagaa gaacaacggg gccauccucu ucgugaagaa cggccuguac uaccugggga     1800

ucaugcccaa gcagaagggc cgguacaagg cccucuccuu cgagccgacg gagaagacca     1860

gcgagggguu cgacaagaug uacuacgacu acuuccccga cgcggccaag augaucccca     1920

agugcuccac ccagcugaag gccgugaccg cccacuucca gacccacacg acccccaucc     1980

ugcucagcaa caacuucauc gagccgcugg agaucaccaa ggagaucuac gaccugaaca     2040

accccgagaa ggagcccaag aaguuccaga ccgccuacgc gaagaagacg ggcgaccaga     2100

agggcuaccg cgaggcccuc ugcaagugga ucgacuucac ccgggacuuc cuguccaagu     2160

acaccaagac cacgagcauc gaccugagcu cccuccgccc cagcucccag uacaaggacc     2220

ugggggagua cuacgccgag cugaacccgc uccuguacca caucagcuuc cagcggaucg     2280

ccgagaagga gaucauggac gccgucgaga ccggcaagcu guaccucuuc cagaucuaca     2340

acaaggacuu cgcgaagggg caccacggca agcccaaccu gcacacccug uacuggaccg     2400

gccucuucuc ccccgagaac cuggccaaga cgagcaucaa gcugaacggg caggccgagc     2460

uguucuaccg ccccaagucc cggaugaagc gcauggccca ccggcugggc gagaagaugc     2520

ugaacaagaa gcucaaggac cagaagaccc cgauccccga cacccuguac caggagcugu     2580

acgacuacgu gaaccaccgc cucagccacg accuguccga cgaggcgcgg gcccugcucc     2640

ccaacgugau caccaaggag gucagccacg agaucaucaa ggaccgccgg uucacguccg     2700

acaaguucuu cuuccacgug ccgaucaccc ugaacuacca ggccgccaac agccccucca     2760

aguucaacca gcgcgucaac gccuaccuga aggagcaccc cgagaccccc aucaucggga     2820

ucgaccgggg cgagcgcaac cucaucuaca ucaccgugau cgacagcacg gggaagaucc     2880

uggagcagcg gucccugaac accauccagc aguucgacua ccagaagaag cucgacaacc     2940

gcgagaagga gcggguggcg gcccgccagg ccuggagcgu cgugggcacc aucaaggacc     3000

ugaagcaggg guaccugucc caggugaucc acgagaucgu cgaccucaug auccacuacc     3060

aggccguggu gguccuggag aaccugaacu ucggcuucaa gagcaagcgc accggcaucg     3120

ccgagaaggc gguguaccag caguucgaga agaugcucau cgacaagcug aacugccugg     3180

ugcucaagga cuacccggcc gagaaggucg ggggcgugcu gaaccccuac cagcugacgg     3240

accaguucac cagcuucgcc aagaugggga cccaguccgg cuuccucuuc uacgugcccg     3300

cccccuacac cagcaagauc gacccgcuga cgggcuucgu cgaccccuuc guguggaaga     3360

ccaucaagaa ccacgagucc cggaagcacu uccuggaggg guucgacuuc cuccacuacg     3420

acgugaagac cggcgacuuc auccugcacu ucaagaugaa ccgcaaccug agcuuccagc     3480

gggggcuccc cggcuucaug cccgccuggg acaucgucuu cgagaagaac gagacccagu     3540

ucgacgcgaa ggggacgccg uucaucgccg gcaagcgcau cgugcccgug aucgagaacc     3600

accgguucac cggccgcuac cgggaccugu accccgccaa cgagcugauc gcccuccugg     3660

aggagaaggg gaucgucuuc cgcgacggcu ccaacauccu gcccaagcuc cuggagaacg     3720

acgacagcca cgcgaucgac accauggugg cccugauccg guccgugcuc cagaugcgca     3780

acagcaacgc cgccaccggg gaggacuaca ucaacucccc gguccgggac cugaacggcg     3840

ugugcuucga cagccgcuuc cagaaccccg aguggcccau ggacgccgac gcgaacgggg     3900

ccuaccacau cgcccugaag ggccagcucc ugcugaacca ccucaaggag uccaaggacc     3960

ugaagcugca gaacggcauc agcaaccagg acuggcucgc cuacauccag gagcugcgga     4020

acaagcgccc cgccgcgacg aagaaggccg ggcaggccaa gaagaagaag ggacuagugc     4080

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     4140

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     4200

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     4260

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     4380

uuucagagcc accagaauu                                                  4399


<210>  2441
<211>  4378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2441
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgagcauc uaccaggagu      120

ucgugaacaa guacucccug agcaagaccc uccgguucga gcugaucccc cagggcaaga      180

cccuggagaa caucaaggcc cgcgggcuca uccuggacga cgagaagcgg gccaaggacu      240

acaagaaggc caagcagauc aucgacaagu accaccaguu cuucaucgag gagauccugu      300

ccagcgugug caucuccgag gaccuccugc agaacuacag cgacgucuac uucaagcuga      360

agaaguccga cgacgacaac cuccagaagg acuucaagag cgccaaggac acgaucaaga      420

agcagaucuc cgaguacauc aaggacagcg agaaguucaa gaaccuguuc aaccagaacc      480

ugaucgacgc gaagaagggc caggaguccg accucauccu guggcugaag cagagcaagg      540

acaacgggau cgagcuguuc aaggccaacu ccgacaucac cgacaucgac gaggcccucg      600

agaucaucaa gagcuucaag ggcuggacca ccuacuucaa gggcuuccac gagaaccgca      660

agaacgugua cuccagcaac gacaucccca cguccaucau cuaccggauc guggacgaca      720

accugcccaa guuccucgag aacaaggcca aguacgagag ccugaaggac aaggcgccgg      780

aggccaucaa cuacgagcag aucaagaagg accuggccga ggagcucacc uucgacaucg      840

acuacaagac cuccgagguc aaccagcgcg uguucagccu ggacgaggug uucgagaucg      900

ccaacuucaa caacuaccug aaccagagcg ggaucaccaa guucaacacg aucaucggcg      960

ggaaguucgu caacggcgag aacaccaagc ggaaggggau caacgaguac aucaaccucu     1020

acucccagca gaucaacgac aagacccuga agaaguacaa gaugagcgug cuguucaagc     1080

agauccucuc cgacaccgag agcaaguccu ucgugaucga caagcuggag gacgacagcg     1140

acgucgugac gaccaugcag uccuucuacg agcagaucgc cgcguucaag accguggagg     1200

agaaguccau caaggagacc cugucccucc uguucgacga ccugaaggcc cagaagcucg     1260

accugagcaa gaucuacuuc aagaacgaca agucccugac ggaccucagc cagcaggucu     1320

ucgacgacua cuccgugauc ggcaccgccg ugcuggagua caucacccag cagaucgccc     1380

ccaagaaccu ggacaacccc agcaagaagg agcaggagcu caucgccaag aagaccgaga     1440

aggcgaagua ccugucccug gagacgauca agcucgcccu ggaggaguuc aacaagcacc     1500

gcgacaucga caagcagugc cgguucgagg agauccuggc caacuucgcc gccaucccca     1560

ugaucuucga cgagaucgcg cagaacaagg acaaccucgc ccagaucagc aucaaguacc     1620

agaaccaggg caagaaggac cugcugcagg ccuccgccga ggacgacguc aaggccauca     1680

aggaccuccu ggaccagacc aacaaccugc ugcacaagcu caagaucuuc cacaucagcc     1740

aguccgagga caaggcgaac auccuggaca aggacgagca cuucuaccug guguucgagg     1800

agugcuacuu cgagcucgcc aacaucgugc cgcuguacaa caagauccgc aacuacauca     1860

cccagaagcc cuacagcgac gagaaguuca agcugaacuu cgagaacagc acccucgcca     1920

acggguggga caagaacaag gagcccgaca acacggccau ccuguucauc aaggacgaca     1980

aguacuaccu gggcgucaug aacaagaaga acaacaagau cuucgacgac aaggcgauca     2040

aggagaacaa gggggagggc uacaagaaga ucguguacaa gcuccugccc ggcgccaaca     2100

agaugcugcc gaagguguuc uucuccgcca agagcaucaa guucuacaac cccuccgagg     2160

acauccuccg gauccgcaac cacagcaccc acaccaagaa cgggagcccc cagaagggcu     2220

acgagaaguu cgaguucaac aucgaggacu gccggaaguu caucgacuuc uacaagcaga     2280

gcaucuccaa gcaccccgag uggaaggacu ucggguuccg cuucagcgac acccagcggu     2340

acaacuccau cgacgaguuc uaccgcgagg ucgagaacca gggcuacaag cugacguucg     2400

agaacaucag cgaguccuac aucgacagcg uggucaacca ggggaagcug uaccucuucc     2460

agaucuacaa caaggacuuc uccgccuaca gcaagggccg gccgaaccug cacacccugu     2520

acuggaaggc ccucuucgac gagcgcaacc ugcaggacgu gguguacaag cugaacggcg     2580

aggcggagcu guucuaccgg aagcagucca uccccaagaa gaucacccac cccgccaagg     2640

aggccaucgc caacaagaac aaggacaacc ccaagaagga gagcgucuuc gaguacgacc     2700

ucaucaagga caagcgcuuc accgaggaca aguucuucuu ccacugcccg aucacgauca     2760

acuucaaguc cagcggggcc aacaaguuca acgacgagau caaccugcuc cugaaggaga     2820

aggcgaacga cgugcacauc cugagcaucg accggggcga gcgccaccuc gccuacuaca     2880

cccuggugga cgggaagggc aacaucauca agcaggacac cuucaacauc aucgggaacg     2940

accgcaugaa gaccaacuac cacgacaagc uggccgccau cgagaaggac cgggacuccg     3000

cccgcaagga cuggaagaag aucaacaaca ucaaggagau gaaggagggc uaccucagcc     3060

aggucgugca cgagaucgcg aagcugguga ucgaguacaa cgccaucguc guguucgagg     3120

accugaacuu cggcuucaag cgggggcgcu ucaaggugga gaagcagguc uaccagaagc     3180

ucgagaagau gcugaucgag aagcugaacu accucguguu caaggacaac gaguucgaca     3240

agacgggcgg ggugcugcgg gccuaccagc ugaccgcccc cuucgagacc uucaagaaga     3300

ugggcaagca gaccgggauc aucuacuacg uccccgcggg cuucaccucc aagaucugcc     3360

ccgugacggg cuucgugaac cagcucuacc cgaaguacga gagcgucucc aagagccagg     3420

aguucuucuc caaguucgac aagaucugcu acaaccugga caagggguac uucgaguuca     3480

gcuucgacua caagaacuuc ggcgacaagg ccgccaaggg gaaguggacc aucgccuccu     3540

ucggcagccg ccugaucaac uuccggaacu ccgacaagaa ccacaacugg gacacccgcg     3600

agguguaccc caccaaggag cucgagaagc ugcugaagga cuacagcauc gaguacggcc     3660

acggggagug caucaaggcc gcgaucugcg gcgaguccga caagaaguuc uucgccaagc     3720

ucacgagcgu gcugaacacc auccugcaga ugcggaacuc caagaccggg accgagcucg     3780

acuaccugau cagccccguc gccgacguga acggcaacuu cuucgacucc cgccaggccc     3840

ccaagaacau gccgcaggac gccgacgcga acggggccua ccacaucggc cugaagggcc     3900

ucaugcugcu ggggcggauc aagaacaacc aggagggcaa gaagcucaac cuggugauca     3960

agaacgagga guacuucgag uucguccaga accgcaacaa caagcggccc gccgccacga     4020

agaaggccgg gcaggcgaag aagaagaagg gacuagugca ucacauuuaa aagcaucuca     4080

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     4140

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     4200

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     4320

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       4378


<210>  2442
<211>  4484
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2442
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgga caagaaguac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccgugaucac cgacgaguac aaggucccca gcaagaaguu caaggugcug gggaacacgg      240

accggcacuc caucaagaag aaccugaucg gcgcccugcu guucgacagc ggcgagaccg      300

ccgaggccac ccgccugaag cggaccgcgc gccggcgcua cacgcggcgc aagaaccgga      360

ucugcuaccu gcaggagauc uucuccaacg agauggccaa gguggacgac agcuucuucc      420

accgccucga ggaguccuuc cuggucgagg aggacaagaa gcacgagcgg caccccaucu      480

ucgggaacau cguggacgag guggccuacc acgagaagua ccccaccauc uaccaccucc      540

gcaagaagcu cgucgacagc accgacaagg ccgaccugcg gcugaucuac cucgcgcugg      600

cccacaugau caaguuccgc ggccacuucc ugaucgaggg ggaccucaac ccggacaacu      660

ccgacgugga caagcuguuc auccagcugg ugcagaccua caaccagcug uucgaggaga      720

accccaucaa cgccagcggc gucgacgcca aggccauccu guccgcgcgg cugagcaagu      780

cccgccggcu cgagaaccug aucgcccagc ugcccgggga gaagaagaac ggccucuucg      840

gcaaccugau cgcccugagc cucgggcuga cgcccaacuu caaguccaac uucgaccugg      900

ccgaggacgc caagcuccag cugagcaagg acaccuacga cgacgaccug gacaaccucc      960

uggcgcagau cggcgaccag uacgccgacc uguuccucgc cgccaagaac cuguccgacg     1020

ccauccugcu cagcgacauc cugcgcguga acaccgagau caccaaggcg ccgcuguccg     1080

ccagcaugau caagcgguac gacgagcacc accaggaccu cacgcugcug aaggcccucg     1140

ugcgccagca gcugcccgag aaguacaagg agaucuucuu cgaccagagc aagaacgggu     1200

acgccggcua caucgacggc ggggccuccc aggaggaguu cuacaaguuc aucaagccca     1260

uccuggagaa gauggacggc accgaggagc uccuggucaa gcugaaccgg gaggaccugc     1320

uccgcaagca gcgcaccuuc gacaacggga gcauccccca ccagauccac cugggcgagc     1380

ugcacgcgau ccuccggcgc caggaggacu ucuacccguu ccugaaggac aaccgggaga     1440

agaucgagaa gauccugacc uuccgcaucc ccuacuacgu ggggccccuc gcccggggca     1500

acucccgcuu cgccuggaug acgcgcaaga gcgaggagac caucaccccc uggaacuucg     1560

aggagguggu cgacaagggc gccuccgcgc agagcuucau cgagcggaug accaacuucg     1620

acaagaaccu gccgaacgag aaggugcugc ccaagcacuc ccuccuguac gaguacuuca     1680

cgguguacaa cgagcugacc aaggucaagu acgugaccga ggggaugcgg aagcccgccu     1740

uccucagcgg cgagcagaag aaggccaucg uggaccugcu guucaagacc aaccgcaagg     1800

ucacggugaa gcagcucaag gaggacuacu ucaagaagau cgagugcuuc gacuccgucg     1860

agaucagcgg gguggaggac cgguucaacg ccucccuggg caccuaccac gaccugcuca     1920

agaucaucaa ggacaaggac uuccuggaca acgaggagaa cgaggacauc cuggaggaca     1980

ucgugcucac ccugacccug uucgaggacc gcgagaugau cgaggagcgg cucaagacgu     2040

acgcccaccu guucgacgac aaggucauga agcagcugaa gcgccggcgc uacaccgggu     2100

ggggccggcu cagccgcaag cugaucaacg gcauccggga caagcagucc gggaagacca     2160

uccuggacuu ccucaagagc gacggcuucg cgaaccgcaa cuucaugcag cugauccacg     2220

acgacucccu gaccuucaag gaggacaucc agaaggccca ggugagcggg cagggcgacu     2280

cccuccacga gcacaucgcc aaccuggccg ggagccccgc caucaagaag ggcauccugc     2340

agacggugaa ggucguggac gagcucguga aggucauggg ccgccacaag ccggagaaca     2400

ucgugaucga gauggcgcgg gagaaccaga ccacccagaa ggggcagaag aacucccgcg     2460

agcggaugaa gcgcaucgag gagggcauca aggagcuggg gagccagauc cugaaggagc     2520

accccgugga gaacacccag cuccagaacg agaagcugua ccuguacuac cuccagaacg     2580

gccgggacau guacgucgac caggagcugg acaucaaccg ccugagcgac uacgacgugg     2640

accacaucgu gccccagucc uuccucaagg acgacagcau cgacaacaag guccugacgc     2700

gguccgacaa gaaccgcggc aagagcgaca acgugcccuc cgaggaggug gucaagaaga     2760

ugaagaacua cuggcggcag cugcucaacg ccaagcugau cacccagcgc aaguucgaca     2820

accugaccaa ggccgagcgc gggggccuca gcgagcugga caaggccggg uucaucaagc     2880

ggcagcuggu ggagacccgg cagaucacca agcacguggc ccagauccuc gacucccgca     2940

ugaacacgaa guacgacgag aacgacaagc ugauccggga ggucaaggug aucacccuca     3000

agagcaagcu cguguccgac uuccgcaagg acuuccaguu cuacaagguc cgggagauca     3060

acaacuacca ccacgcgcac gacgccuacc ugaacgccgu ggugggcacc gcccugauca     3120

agaaguaccc gaagcucgag agcgaguucg ucuacgggga cuacaaggug uacgacgugc     3180

gcaagaugau cgcgaagucc gagcaggaga ucggcaaggc caccgccaag uacuucuucu     3240

acagcaacau caugaacuuc uucaagacgg agaucacccu ggccaacggc gagauccgga     3300

agcgcccccu gaucgagacc aacggggaga ccggcgagau cgucugggac aaggggcggg     3360

acuucgccac ggugcgcaag gugcucucca ugccccaggu caacaucgug aagaagaccg     3420

aggugcagac cggcggguuc agcaaggagu ccauccugcc caagcgcaac agcgacaagc     3480

ugaucgcgcg gaagaaggac ugggacccga agaaguacgg cggcuucgac ucccccaccg     3540

ucgccuacag cgugcucgug gucgccaagg uggagaaggg caagagcaag aagcugaagu     3600

ccgugaagga gcugcucggg aucacgauca uggagcgcag cuccuucgag aagaacccca     3660

ucgacuuccu ggaggccaag ggguacaagg aggucaagaa ggaccugauc aucaagcucc     3720

cgaaguacag ccuguucgag cuggagaacg gccggaagcg caugcucgcc uccgcggggg     3780

agcugcagaa gggcaacgag cuggcccucc ccagcaagua cgugaacuuc cuguaccugg     3840

ccucccacua cgagaagcuc aagggcagcc ccgaggacaa cgagcagaag cagcuguucg     3900

uggagcagca caagcacuac cuggacgaga ucaucgagca gaucuccgag uucagcaagc     3960

gggucauccu cgccgacgcc aaccuggaca aggugcuguc cgcguacaac aagcaccgcg     4020

acaagcccau ccgggagcag gccgagaaca ucauccaccu cuucacccug accaaccugg     4080

gggccccggc cgcguucaag uacuucgaca ccacgaucga ccgcaagcgg uacaccagca     4140

ccaaggaggu gcucgacgcc acccugaucc accaguccau cacgggccug uacgagaccc     4200

gcaucgaccu cagccagcuc gggggcgaca agcggcccgc cgccaccaag aaggccgggc     4260

aggcgaagaa gaagaaguaa ggacuaguaa uaaggaaacu guaugaaugu cugugggcag     4320

gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu     4380

uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4484


<210>  2443
<211>  5267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2443
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa cuucaagauc cugcccaucg ccaucgaccu cggcgugaag aacaccgggg      180

uguucagcgc cuucuaccag aagggcaccu cccuggagcg gcuggacaac aagaacggga      240

aggucuacga gcucagcaag gacuccuaca cgcugcugau gaacaaccgc accgcccggc      300

gccaccagcg gcgcggcauc gaccggaagc agcucgugaa gcgccuguuc aagcugaucu      360

ggaccgagca gcucaaccug gagugggaca aggacaccca gcaggccauc agcuuccugu      420

ucaaccggcg cggcuucucc uucaucacgg acggguacag ccccgaguac cucaacaucg      480

ugcccgagca ggucaaggcg auccugaugg acaucuucga cgacuacaac ggcgaggacg      540

accuggacuc cuaccucaag cuggccaccg agcaggagag caagaucucc gagaucuaca      600

acaagcugau gcagaagauc cucgaguuca agcugaugaa gcugugcacc gacaucaagg      660

acgacaaggu gagcaccaag acgcucaagg agaucaccuc cuacgaguuc gagcugcugg      720

ccgacuaccu cgccaacuac agcgaguccc ugaagaccca gaaguucagc uacaccgaca      780

agcaggggaa ccugaaggag cucuccuacu accaccacga caaguacaac auccaggagu      840

uccugaagcg gcacgcgacg aucaacgacc gcauccugga cacccuccug accgacgacc      900

uggacaucug gaacuucaac uucgagaagu ucgacuucga caagaacgag gagaagcucc      960

agaaccagga ggacaaggac cacauccagg cccaccucca ccacuucgug uucgccguca     1020

acaagaucaa gagcgagaug gccuccggcg ggcggcaccg cagccaguac uuccaggaga     1080

ucaccaacgu gcuggacgag aacaaccacc aggagggcua ccugaagaac uucugcgaga     1140

accugcacaa caagaaguac uccaaccuga gcgugaagaa ccucgucaac cugaucggca     1200

accugagcaa ccucgagcug aagccgcugc ggaaguacuu caacgacaag auccacgcca     1260

aggcggacca cugggacgag cagaaguuca cggagaccua cugccacugg auccucgggg     1320

aguggcgcgu gggcgugaag gaccaggaca agaaggacgg ggccaaguac uccuacaagg     1380

accugugcaa cgagcugaag cagaagguca ccaaggccgg ccucguggac uuccugcugg     1440

agcuggaccc cugccggacc aucccgcccu accucgacaa caacaaccgc aagccgccca     1500

agugccagag ccugauccug aaccccaagu uccucgacaa ccaguacccc aacuggcagc     1560

aguaccugca ggagcugaag aagcuccagu ccauccagaa cuaccuggac agcuucgaga     1620

cggaccugaa ggugcucaag uccagcaagg accagcccua cuucgucgag uacaagucca     1680

gcaaccagca gaucgccucc ggccagcggg acuacaagga ccuggacgcc cgcauccugc     1740

aguucaucuu cgaccgggug aaggcgagcg acgagcuccu gcugaacgag aucuacuucc     1800

aggccaagaa gcucaagcag aaggccucca gcgagcugga gaagcuggag uccagcaaga     1860

agcucgacga ggugaucgcc aacucccagc ugagccagau ccugaagucc cagcacacca     1920

acgggaucuu cgagcagggc accuuccucc accuggucug caaguacuac aagcagcgcc     1980

agcgcgcccg ggacagccgc cuguacauca ugcccgagua ccgguacgac aagaagcucc     2040

acaaguacaa caacaccggg cgcuucgacg acgacaacca gcugcugacg uacugcaacc     2100

acaagccccg gcagaagcgc uaccagcucc ugaacgaccu ggcgggcgug cuccaggugu     2160

cccccaacuu ccugaaggac aagaucggga gcgacgacga ccuguucauc agcaaguggc     2220

ucgucgagca cauccggggc uucaagaagg ccugcgagga cucccugaag auccagaagg     2280

acaaccgcgg ccugcucaac cacaagauca acaucgcccg gaacaccaag gggaagugcg     2340

agaaggagau cuucaaccug aucugcaaga ucgagggcag cgaggacaag aaggggaacu     2400

acaagcacgg ccuggccuac gagcucgggg ugcugcuguu cggcgagccg aacgaggccu     2460

ccaagcccga guucgaccgc aagaucaaga aguucaacag caucuacucc uucgcgcaga     2520

uccagcagau cgccuucgcc gagcggaagg gcaacgccaa cacgugcgcg gucugcagcg     2580

ccgacaacgc ccaccgcaug cagcagauca agaucaccga gcccguggag gacaacaagg     2640

acaagaucau ccucuccgcc aaggcccagc ggcugcccgc gaucccgacc cgcaucgugg     2700

acggggccgu caagaagaug gccaccaucc uggccaagaa caucguggac gacaacuggc     2760

agaacaucaa gcagguccuc agcgccaagc accagcugca cauccccauc aucaccgagu     2820

ccaacgcguu cgaguucgag cccgcccugg ccgacgugaa gggcaagagc cucaaggacc     2880

ggcgcaagaa ggcccuggag cggaucuccc ccgagaacau cuucaaggac aagaacaacc     2940

gcaucaagga guucgccaag gggaucagcg cguacuccgg cgccaaccug accgacgggg     3000

acuucgacgg cgccaaggag gagcucgacc acaucauccc gcggagccac aagaaguacg     3060

gcacgcugaa cgacgaggcc aaccugaucu gcgugacccg cggggacaac aagaacaagg     3120

gcaaccgcau cuucugccuc cgggaccugg cggacaacua caagcugaag caguucgaga     3180

ccaccgacga ccucgagauc gagaagaaga ucgccgacac gaucugggac gccaacaaga     3240

aggacuucaa guucgggaac uaccgcuccu ucaucaaccu gacgccccag gagcagaagg     3300

ccuuccggca cgcccuguuc cucgcggacg agaaccccau caagcaggcc gugauccgcg     3360

ccaucaacaa ccggaaccgc accuucguca acggcaccca gcgguacuuc gccgaggugc     3420

uggccaacaa caucuaccug cgcgcgaaga aggagaaccu caacaccgac aagaucagcu     3480

ucgacuacuu cggcaucccc accaucggga acggccgggg gaucgccgag auccgccagc     3540

uguacgagaa gguggacucc gacauccagg ccuacgccaa gggcgacaag ccgcaggcca     3600

gcuacagcca ccugaucgac gcgaugcucg ccuucugcau cgccgccgac gagcaccgga     3660

acgacggguc caucggccug gagaucgaca agaacuacag ccuguacccc cucgacaaga     3720

acaccggcga ggucuucacc aaggacaucu ucucccagau caagaucacc gacaacgagu     3780

ucagcgacaa gaagcuggug cgcaagaagg cgaucgaggg guucaacacg caccggcaga     3840

ugacccgcga cggcaucuac gccgagaacu accugcccau ccucauccac aaggagcuga     3900

acgaggugcg gaagggguac accuggaaga acuccgagga gaucaagauc uucaagggca     3960

agaaguacga cauccagcag cugaacaacc ucgucuacug ccugaaguuc guggacaagc     4020

cgaucagcau cgacauccag aucuccaccc uggaggagcu ccgcaacauc cugacgacca     4080

acaacaucgc cgccaccgcc gaguacuacu acaucaaccu gaagacccag aagcuccacg     4140

aguacuacau cgagaacuac aacacggcgc ugggguacaa gaaguacagc aaggagaugg     4200

aguuccugcg gucccucgcc uaccgcagcg agcgggugaa gaucaagucc aucgacgacg     4260

ucaagcaggu ccuggacaag gacagcaacu ucaucaucgg caagaucacc cugcccuuca     4320

agaaggagug gcagcgccuc uaccgggagu ggcagaacac caccaucaag gacgacuacg     4380

aguuccugaa guccuucuuc aacgucaaga gcaucacgaa gcugcacaag aaggugcgca     4440

aggacuucuc ccuccccauc agcaccaacg agggcaaguu ccuggugaag cgcaagaccu     4500

gggacaacaa cuucaucuac cagauccuga acgacuccga cagccgggcc gacgggacca     4560

agcccuucau cccggccuuc gacaucucca agaacgagau cguggaggcc aucaucgaca     4620

gcuucacgag caagaacauc uucuggcucc ccaagaacau cgagcugcag aagguggaca     4680

acaagaacau cuucgcgauc gacaccucca agugguucga gguggagacc cccagcgacc     4740

ugcgcgacau cggcaucgcc accauccagu acaagaucga caacaacucc cggcccaagg     4800

ugcgcgucaa gcuggacuac gugaucgacg acgacagcaa gaucaacuac uucaugaacc     4860

acucccuccu caagagccgg uacccggaca aggugcugga gauccugaag caguccacga     4920

ucaucgaguu cgagagcucc ggguucaaca agaccaucaa ggagaugcug ggcaugaagc     4980

uggccgggau cuacaacgag accagcaaca acaagcgccc cgccgccacc aagaaggcgg     5040

gccaggccaa gaagaagaag uaaggacuag uaauaaggaa acuguaugaa ugucuguggg     5100

caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug aauaauaaac     5160

uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa aaaaaaaaaa     5220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   5267


<210>  2444
<211>  3539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2444
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gcggaacuac auccugggcc ucgacaucgg gaucaccagc gugggcuacg      180

ggaucaucga cuacgagacc cgcgacguga ucgacgccgg cguccggcug uucaaggagg      240

ccaacgugga gaacaacgag ggccgccggu ccaagcgcgg ggcccggcgc cugaagcggc      300

gccggcgcca ccggauccag cgcgugaaga agcuccuguu cgacuacaac cugcucacgg      360

accacagcga gcuguccggc aucaaccccu acgaggcccg ggucaagggg cugagccaga      420

agcucuccga ggaggaguuc agcgcggccc ugcugcaccu cgccaagcgc cggggcgugc      480

acaacgugaa cgaggucgag gaggacaccg ggaacgagcu guccaccaag gagcagauca      540

gccgcaacuc caaggcccug gaggagaagu acguggcgga gcuccagcug gagcggcuga      600

agaaggacgg cgaggugcgc ggcagcauca accgguucaa gaccuccgac uacgucaagg      660

aggccaagca gcuccugaag gugcagaagg ccuaccacca gcuggaccag agcuucaucg      720

acacguacau cgaccuccug gagacccgcc gcaccuacua cgaggggccc ggcgaggggu      780

cccccuucgg cuggaaggac aucaaggagu gguacgagau gcugaugggc cacugcaccu      840

acuucccgga ggagcuccgg agcgugaagu acgccuacaa cgccgaccug uacaacgcgc      900

ugaacgaccu caacaaccug gucaucacgc gcgacgagaa cgagaagcug gaguacuacg      960

agaaguucca gaucaucgag aacguguuca agcagaagaa gaagcccacc cucaagcaga     1020

ucgccaagga gauccuggug aacgaggagg acaucaaggg guaccggguc accuccaccg     1080

gcaagcccga guucacgaac cugaaggugu accacgacau caaggacauc accgcccgca     1140

aggagaucau cgagaacgcc gagcuccugg accagaucgc caagauccug accaucuacc     1200

agagcuccga ggacauccag gaggagcuca ccaaccugaa cagcgagcug acgcaggagg     1260

agaucgagca gaucuccaac cucaaggggu acaccggcac ccacaaccug agccugaagg     1320

cgaucaaccu cauccuggac gagcuguggc acaccaacga caaccagauc gccaucuuca     1380

accggcucaa gcuggugccc aagaaggucg accugagcca gcagaaggag aucccgacga     1440

cccucgugga cgacuucauc cugucccccg uggucaagcg cagcuucauc caguccauca     1500

aggugaucaa cgccaucauc aagaaguacg ggcugcccaa cgacaucauc aucgagcugg     1560

cccgggagaa gaacagcaag gacgcccaga agaugaucaa cgagaugcag aagcgcaacc     1620

ggcagaccaa cgagcgcauc gaggagauca uccggaccac gggcaaggag aacgcgaagu     1680

accucaucga gaagaucaag cugcacgaca ugcaggaggg caagugccug uacucccucg     1740

aggccauccc ccuggaggac cugcucaaca acccguucaa cuacgagguc gaccacauca     1800

ucccccgcag cguguccuuc gacaacagcu ucaacaacaa ggugcugguc aagcaggagg     1860

agaacuccaa gaaggggaac cggacccccu uccaguaccu gagcuccagc gacuccaaga     1920

ucagcuacga gaccuucaag aagcacaucc ucaaccuggc caagggcaag gggcgcaucu     1980

ccaagaccaa gaaggaguac cugcucgagg agcgggacau caaccgcuuc agcgugcaga     2040

aggacuucau caaccggaac cugguggaca cgcgcuacgc cacccggggc cugaugaacc     2100

uccugcgcuc cuacuuccgc gucaacaacc uggacgugaa ggucaagagc aucaacgggg     2160

gcuucaccuc cuuccuccgg cgcaagugga aguucaagaa ggagcggaac aagggcuaca     2220

agcaccacgc cgaggacgcg cugaucaucg ccaacgccga cuucaucuuc aaggagugga     2280

agaagcugga caaggccaag aaggugaugg agaaccagau guucgaggag aagcaggcgg     2340

agagcaugcc cgagaucgag accgagcagg aguacaagga gaucuucauc acgccgcacc     2400

agaucaagca caucaaggac uucaaggacu acaaguacag ccaccgcgug gacaagaagc     2460

ccaaccggga gcucaucaac gacacccugu acuccacccg caaggacgac aaggggaaca     2520

cccugaucgu gaacaaccuc aacggccugu acgacaagga caacgacaag cugaagaagc     2580

ucaucaacaa gagccccgag aagcugcuga uguaccacca cgacccccag acguaccaga     2640

agcucaagcu gaucauggag caguacgggg acgagaagaa cccgcuguac aaguacuacg     2700

aggagaccgg caacuaccuc accaaguacu ccaagaagga caacgggccc gucaucaaga     2760

agaucaagua cuacggcaac aagcugaacg cccaccugga caucaccgac gacuacccca     2820

acagccggaa caagguggug aagcucuccc ugaagcccua ccgcuucgac gucuaccugg     2880

acaacggcgu guacaaguuc gugaccguca agaaccucga cgugaucaag aaggagaacu     2940

acuacgaggu gaacagcaag ugcuacgagg aggccaagaa gcugaagaag aucuccaacc     3000

aggccgaguu caucgccagc uucuacaaca acgaccugau caagaucaac ggggagcucu     3060

accgggucau cggcgugaac aacgaccugc ugaaccgcau cgaggugaac augaucgaca     3120

ucacguaccg ggaguaccuc gagaacauga acgacaagcg cccgccccgg aucaucaaga     3180

ccaucgcguc caagacccag agcaucaaga aguacuccac cgacauccug gggaaccugu     3240

acgaggucaa gagcaagaag cacccccaga ucaucaagaa gggcaagcgc cccgccgcca     3300

cgaagaaggc cggccaggcc aagaagaaga aguaaggacu aguaauaagg aaacuguaug     3360

aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa uuaugucugc     3420

ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      3539


<210>  2445
<211>  4607
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2445
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgcu guucaacaag ugcaucauca ucagcaucaa ccucgacuuc uccaacaagg      180

agaagugcau gaccaagccc uacagcaucg gccuggacau cgggaccaac uccgugggcu      240

gggccgugau cacggacaac uacaaggucc ccagcaagaa gaugaaggug cuggggaaca      300

ccuccaagaa guacaucaag aagaaccucc ugggcgugcu gcuguucgac agcggcauca      360

ccgccgaggg gcggcgccug aagcggaccg cccgccggcg cuacacgcgg cgccggaacc      420

gcauccugua ccuccaggag aucuucucca ccgagauggc cacccuggac gacgcguucu      480

uccagcggcu ggacgacagc uuccucgucc ccgacgacaa gcgcgacucc aaguacccga      540

ucuucggcaa ccugguggag gagaaggugu accacgacga guuccccacc aucuaccacc      600

uccggaagua ccucgccgac agcacgaaga aggccgaccu gcgccugguc uaccucgccc      660

uggcgcacau gaucaaguac cgggggcacu uccugaucga gggcgaguuc aacuccaaga      720

acaacgacau ccagaagaac uuccaggacu uccucgacac cuacaacgcc aucuucgaga      780

gcgaccuguc ccuggagaac agcaagcagc ucgaggagau cgugaaggac aagaucucca      840

agcuggagaa gaaggaccgc auccugaagc uguuccccgg ggagaagaac agcggcaucu      900

ucuccgaguu ccugaagcug aucgugggca accaggccga cuuccggaag ugcuucaacc      960

ucgacgagaa ggccagccug cacuucucca aggagagcua cgacgaggac cuggagaccc     1020

uccuggggua caucggcgac gacuacagcg acgucuuccu gaaggccaag aagcucuacg     1080

acgcgauccu gcuguccggg uuccucaccg ugacggacaa cgagaccgag gcgccccuga     1140

gcuccgccau gaucaagcgc uacaacgagc acaaggagga ccuggcccuc cugaaggagu     1200

acauccggaa caucagccug aagaccuaca acgagguguu caaggacgac accaagaacg     1260

gcuacgccgg cuacaucgac gggaagacga accaggagga cuucuacguc uaccucaaga     1320

accugcuggc cgaguucgag ggcgccgacu acuuccucga gaagaucgac cgcgaggacu     1380

uccugcggaa gcagcgcacc uucgacaacg gguccauccc guaccagauc caccuccagg     1440

agaugcgcgc cauccuggac aagcaggcca aguucuaccc cuuccucgcc aagaacaagg     1500

agcggaucga gaagauccug accuuccgca uccccuacua cgugggcccc cuggcgcggg     1560

ggaacagcga cuucgccugg uccauccgca agcggaacga gaagaucacc ccguggaacu     1620

ucgaggacgu gaucgacaag gagagcuccg ccgaggccuu caucaaccgc augacgagcu     1680

ucgaccucua ccugcccgag gagaaggucc ugcccaagca cucccuccug uacgagaccu     1740

ucaacgugua caacgagcug accaaggugc gguucaucgc cgagagcaug cgcgacuacc     1800

aguuccucga cuccaagcag aagaaggaca ucguccggcu guacuucaag gacaagcgca     1860

aggugaccga caaggacauc aucgaguacc ugcacgcgau cuacggcuac gacggcaucg     1920

agcucaaggg gaucgagaag caguucaaca gcucccugag cacguaccac gaccugcuca     1980

acaucaucaa cgacaaggag uuccuggacg acuccagcaa cgaggccauc aucgaggaga     2040

ucauccacac ccugaccauc uucgaggacc gggagaugau caagcagcgc cucuccaagu     2100

ucgagaacau cuucgacaag agcgugcuga agaagcugag ccggcgccac uacaccggcu     2160

gggggaagcu cuccgccaag cugaucaacg gcauccggga cgagaagucc gggaacacga     2220

uccuggacua ccucaucgac gacggcauca gcaaccgcaa cuucaugcag cugauccacg     2280

acgacgcccu gagcuucaag aagaagaucc agaaggcgca gaucaucggc gacgaggaca     2340

aggggaacau caaggagguc gugaaguccc uccccggcag cccggccauc aagaagggga     2400

uccugcaguc caucaagauc gucgacgagc uggugaaggu gaugggcggg cggaagcccg     2460

agagcaucgu cguggagaug gcccgcgaga accaguacac caaccagggc aaguccaaca     2520

gccagcagcg gcucaagcgc cuggagaagu cccugaagga gcucggcagc aagauccuga     2580

aggagaacau ccccgccaag cuguccaaga ucgacaacaa cgcccuccag aacgaccgcc     2640

uguaccugua cuaccuccag aacgggaagg acauguacac cggcgacgac cuggacaucg     2700

accggcugag caacuacgac aucgaccaca ucauccccca ggcguuccuc aaggacaacu     2760

ccaucgacaa caaggugcug gucagcuccg ccagcaaccg cgggaagucc gacgacuucc     2820

cgagccugga gguggugaag aagcggaaga ccuucuggua ccagcuccuc aagagcaagc     2880

ugaucuccca gcgcaaguuc gacaaccuga cgaaggccga gcggggcggc cugcugcccg     2940

aggacaaggc cggguucauc cagcgccagc ucgucgagac ccggcagauc accaagcacg     3000

uggcccgccu gcuggacgag aaguucaaca acaagaagga cgagaacaac cgggcggugc     3060

gcaccgucaa gaucaucacg cucaagagca cccugguguc ccaguuccgg aaggacuucg     3120

agcuguacaa ggugcgcgag aucaacgacu uccaccacgc ccacgacgcc uaccucaacg     3180

ccgucaucgc cagcgcgcug cugaagaagu accccaagcu cgagcccgag uucguguacg     3240

gcgacuaccc gaaguacaac uccuuccggg agcgcaagag cgccaccgag aagguguacu     3300

ucuacuccaa caucaugaac aucuucaaga aguccaucag ccuggccgac gggcggguca     3360

ucgagcgccc ccugaucgag gugaacgagg agaccggcga gagcgugugg aacaaggagu     3420

ccgaccucgc caccguccgg cgcgugcuga gcuaccccca ggugaacguc gugaagaagg     3480

uggaggagca gaaccacggg cuggaccggg gcaagcccaa gggccucuuc aacgcgaacc     3540

uguccagcaa gccgaagccc aacuccaacg agaaccuggu cggggccaag gaguaccucg     3600

accccaagaa guacggcggg uacgccggca ucagcaacuc cuucgccgug cuggugaagg     3660

ggacgaucga gaagggcgcc aagaagaaga ucaccaacgu ccuggaguuc cagggcauca     3720

gcauccucga ccgcaucaac uaccggaagg acaagcugaa cuuccugcuc gagaaggggu     3780

acaaggacau cgagcugauc aucgagcugc cgaaguacag ccucuucgag cuguccgacg     3840

gcagccgccg caugcuggcg uccauccuca gcaccaacaa caagcgcggg gagauccaca     3900

agggcaacca gaucuuccug ucccagaagu ucgugaagcu gcucuaccac gccaagcgga     3960

ucagcaacac caucaacgag aaccaccgga aguacgugga gaaccacaag aaggaguucg     4020

aggagcuguu cuacuacauc cuggaguuca acgagaacua cgucggggcc aagaagaacg     4080

gcaagcuccu gaacuccgcc uuccagagcu ggcagaacca cuccaucgac gagcugugca     4140

gcuccuucau cggccccacg gggagcgagc gcaagggccu cuucgagcug accucccggg     4200

ggagcgccgc ggacuucgag uuccugggcg ugaagauccc ccgcuaccgg gacuacaccc     4260

ccuccagccu ccugaaggac gccacccuga uccaccaguc cgugacgggg cuguacgaga     4320

cccgcaucga ccucgccaag cugggcgagg gcaagcggcc ggccgccacc aagaaggcgg     4380

ggcaggccaa gaagaagaag uaaggacuag uaauaaggaa acuguaugaa ugucuguggg     4440

caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug aauaauaaac     4500

uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   4607


<210>  2446
<211>  3683
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2446
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgug guacgccagc cugauguccg cccaccaccu ccgggugggc aucgacgugg      180

ggacccacag cgucggccug gccacccugc gcguggacga ccacgggacg cccaucgagc      240

uccuguccgc ccugagccac auccacgacu ccggcguggg caaggagggg aagaaggacc      300

acgacacccg gaagaagcuc agcggcaucg cgcgccgggc ccgccggcug cugcaccacc      360

gccggaccca gcuccagcag cuggacgagg uccugcgcga ccucggguuc cccaucccca      420

ccccgggcga guuccuggac cugaacgagc agacggaccc cuaccgggug uggcgcgugc      480

gggcccgccu cgucgaggag aagcugcccg aggagcugcg ggggcccgcc aucuccaugg      540

cggugcgcca caucgcccgg caccgcggcu ggcggaaccc guacagcaag guggaguccc      600

uccugagccc cgccgaggag ucccccuuca ugaaggcccu gcgcgagcgg auccucgcca      660

ccaccggcga gguccuggac gacgggauca cccccggcca ggcgauggcc cagguggccc      720

ugacgcacaa caucagcaug cgcgggccgg agggcauccu cggcaagcug caccaguccg      780

acaacgccaa cgagauccgc aagaucugcg cccggcaggg ggugagcccc gacgucugca      840

agcagcugcu ccgcgcggug uucaaggccg acagcccccg gggcuccgcc gugucccgcg      900

ucgcccccga cccgcugccc gggcagggca gcuuccggcg cgcccccaag ugcgaccccg      960

aguuccagcg guuccgcauc aucuccaucg uggcgaaccu gcggaucagc gagaccaagg     1020

gggagaaccg cccgcucacc gccgacgagc ggcgccacgu ggucaccuuc cugacggagg     1080

acagccaggc cgaccugacc uggguggacg uggccgagaa gcucggcguc caccggcgcg     1140

accugcgggg caccgccgug cacaccgacg acggggagcg cuccgcggcc cggccgccca     1200

ucgacgccac ggaccgcauc augcggcaga ccaagaucag cucccugaag accugguggg     1260

aggaggccga cagcgagcag cgcggcgcga ugauccgcua ccucuacgag gaccccaccg     1320

acuccgagug cgccgagauc aucgccgagc ugcccgagga ggaccaggcc aagcuggaca     1380

gccuccaccu ccccgccggg cgggcggccu acucccgcga gagccugacg gcccuguccg     1440

accacaugcu ggccaccacc gacgaccugc acgaggcccg gaagcgccuc uucggcgucg     1500

acgacagcug ggcgccgccc gccgaggcca ucaacgcccc cguggggaac cccuccgugg     1560

accggacccu gaagaucguc ggccgcuacc ugagcgccgu ggaguccaug uggggcacgc     1620

ccgaggugau ccacgucgag cacgugcggg acggguucac cagcgagcgc auggcggacg     1680

agcgggacaa ggccaaccgc cggcgcuaca acgacaacca ggaggccaug aagaagaucc     1740

agcgggacua cggcaaggag ggguacaucu cccgcggcga caucgugcgg cucgacgccc     1800

uggagcugca ggggugcgcg ugccuguacu gcggcaccac caucggcuac cacacgugcc     1860

agcucgacca caucgucccg caggccgggc ccggcagcaa caaccgccgg gggaaccugg     1920

uggccgugug cgagcgcugc aaccggucca agagcaacac ccccuucgcc gucugggccc     1980

agaagugcgg caucccccac gugggcguga aggaggcgau cgggcgcguc cggggcuggc     2040

gcaagcagac cccgaacacc uccagcgagg accugacgcg ccucaagaag gaggugaucg     2100

cccggcugcg ccggacccag gaggaccccg agaucgacga gcgcagcaug gaguccgugg     2160

ccuggauggc caacgagcug caccaccgga ucgccgcggc cuaccccgag accaccguca     2220

ugguguaccg cgggagcauc acggccgccg cccggaaggc ggccggcauc gacucccgca     2280

ucaaccucau cggggagaag ggccggaagg accgcaucga ccggcgccac cacgccgugg     2340

acgccagcgu cguggcgcug auggaggccu ccguggccaa gacccuggcc gagcggagcu     2400

cccuccgcgg cgagcagcgg cugaccggga aggagcagac cuggaagcag uacaccggca     2460

gcacggucgg ggcccgcgag cacuucgaga uguggcgggg ccacaugcug caccucaccg     2520

agcuguucaa cgagcgccug gcggaggaca agguguacgu gacccagaac auccggcucc     2580

gccuguccga cgggaacgcc cacaccguca accccagcaa gcuggugucc caccgccucg     2640

gcgacggccu gacggugcag cagaucgacc gggccugcac cccggcccug uggugcgccc     2700

ucacccgcga gaaggacuuc gacgagaaga acgggcugcc cgcgcgggag gaccgcgcca     2760

uccgggucca cggccacgag aucaagagcu ccgacuacau ccagguguuc agcaagcgca     2820

agaagaccga cuccgaccgg gacgagacgc ccuucggggc caucgccgug cgcggcgggu     2880

ucgucgagau cggccccagc auccaccacg cccggaucua ccgcguggag ggcaagaagc     2940

cgguguacgc gaugcugcgg gucuucaccc acgaccuccu gucccagcgc cacggggacc     3000

uguucagcgc cgugaucccg ccccagagca ucuccaugcg gugcgccgag cccaagcucc     3060

gcaaggccau caccaccggc aacgccacgu accuggggug gguggucgug ggcgacgagc     3120

uggagaucaa cguggacagc uucaccaagu acgcgaucgg gcgguuccuc gaggacuucc     3180

ccaacaccac ccgcuggcgg aucugcggcu acgacacgaa cuccaagcug acccugaagc     3240

ccaucguccu cgccgccgag ggccuggaga accccagcuc cgccgugaac gagaucgugg     3300

agcugaaggg guggcgcguc gcgaucaacg ugcucaccaa ggugcacccc accgucgugc     3360

ggcgcgacgc ccugggccgg ccgcgcuaca gcucccgcag caaccugccc acguccugga     3420

ccaucgagaa gcggcccgcc gccaccaaga aggccgggca ggcgaagaag aagaaguaag     3480

gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag auccuucugu     3540

aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau aaaucuggua     3600

gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaga auu                                             3683


<210>  2447
<211>  4544
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2447
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgac caagcccuac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccgugacgac cgacaacuac aaggucccca gcaagaagau gaaggugcuc gggaacaccu      240

ccaagaagua caucaagaag aaccugcugg gcgugcuccu guucgacagc ggcaucaccg      300

ccgaggggcg gcgccugaag cggacggccc gccggcgcua cacccggcgc cggaaccgca      360

uccucuaccu gcaggagauc uucuccaccg agauggccac ccuggacgac gcguucuucc      420

agcggcucga cgacagcuuc cugguccccg acgacaagcg cgacuccaag uacccgaucu      480

ucggcaaccu gguggaggag aaggccuacc acgacgaguu ccccacgauc uaccaccucc      540

ggaaguaccu ggccgacagc accaagaagg ccgaccugcg ccucguguac cuggcgcugg      600

cccacaugau caaguaccgg gggcacuucc ucaucgaggg cgaguucaac uccaagaaca      660

acgacaucca gaagaacuuc caggacuucc uggacaccua caacgccauc uucgagagcg      720

accugucccu cgagaacagc aagcagcugg aggagaucgu caaggacaag aucuccaagc      780

uggagaagaa ggaccgcauc cucaagcugu uccccgggga gaagaacagc ggcaucuucu      840

ccgaguuccu gaagcucauc gugggcaacc aggccgacuu ccggaagugc uucaaccugg      900

acgagaaggc cagccugcac uucuccaagg agagcuacga cgaggaccuc gagacccugc      960

ugggguacau cggcgacgac uacuccgacg uguuccucaa ggcgaagaag cuguacgacg     1020

ccauccugcu cagcggguuc cugacgguca ccgacaacga gaccgaggcg ccccugagcu     1080

ccgccaugau caagcgcuac aacgagcaca aggaggaccu cgcccugcug aaggaguaca     1140

uccggaacau cagccucaag accuacaacg agguguucaa ggacgacacg aagaacggcu     1200

acgccggcua caucgacggg aagaccaacc aggaggacuu cuacguguac cugaagaagc     1260

ugcucgccga guucgagggc gccgacuacu uccuggagaa gaucgaccgc gaggacuucc     1320

ugcggaagca gcgcaccuuc gacaacgggu ccaucccgua ccagauccac cuccaggaga     1380

ugcgcgccau ccuggacaag caggccaagu ucuaccccuu ccuggcgaag aacaaggagc     1440

ggaucgagaa gauccugacc uuccgcaucc ccuacuacgu cggcccccuc gcccggggga     1500

acagcgacuu cgccuggucc auccgcaagc ggaacgagaa gaucacgccg uggaacuucg     1560

aggacgugau cgacaaggag agcuccgccg aggccuucau caaccgcaug accagcuucg     1620

accuguaccu gcccgaggag aaggugcucc ccaagcacuc ccugcuguac gagaccuuca     1680

acgucuacaa cgagcucacc aaggugcggu ucaucgcgga gagcaugcgc gacuaccagu     1740

uccuggacuc caagcagaag aaggacaucg ugcggcugua cuucaaggac aagcgcaagg     1800

ucacggacaa ggacaucauc gaguaccucc acgccaucua cggcuacgac ggcaucgagc     1860

ugaaggggau cgagaagcag uucaacagcu cccugagcac cuaccacgac cuccugaaca     1920

ucaucaacga caaggaguuc cuggacgacu ccagcaacga ggccaucauc gaggagauca     1980

uccacacccu caccaucuuc gaggaccggg agaugaucaa gcagcgccug uccaaguucg     2040

agaacaucuu cgacaagagc gugcugaaga agcucucccg gcgccacuac acgggcuggg     2100

ggaagcugag cgccaagcug aucaacggca uccgggacga gaaguccggg aacaccaucc     2160

ucgacuaccu gaucgacgac ggcaucagca accgcaacuu caugcagcug auccacgacg     2220

acgcgcucag cuucaagaag aagauccaga aggcccagau caucggcgac gaggacaagg     2280

ggaacaucaa ggaggugguc aagucccugc ccggcagccc ggccaucaag aaggggaucc     2340

ugcaguccau caagaucgug gacgagcucg ucaaggugau gggcgggcgg aagcccgaga     2400

gcaucguggu cgagauggcc cgcgagaacc aguacaccaa ccagggcaag uccaacagcc     2460

agcagcggcu gaagcgccug gagaaguccc ucaaggagcu gggcagcaag auccugaagg     2520

agaacauccc cgccaagcuc uccaagaucg acaacaacgc gcugcagaac gaccgccugu     2580

accucuacua ccugcagaac gggaaggaca uguacaccgg cgacgaccug gacaucgacc     2640

ggcucagcaa cuacgacauc gaccacauca ucccccaggc cuuccugaag gacaacucca     2700

ucgacaacaa ggugcuggug agcuccgcca gcaaccgcgg gaaguccgac gacgucccga     2760

gccucgaggu ggugaagaag cggaagacgu ucugguacca gcugcucaag agcaagcuga     2820

ucucccagcg caaguucgac aaccugacca aggccgagcg gggcggccug agccccgagg     2880

acaaggccgg guucauccag cgccagcucg ucgagacccg gcagaucacc aagcacgugg     2940

cgcgccugcu ggacgagaag uucaacaaca agaaggacga gaacaaccgg gccgugcgca     3000

cggucaagau caucacccuc aaguccaccc uggugagcca guuccggaag gacuucgagc     3060

uguacaaggu gcgcgagauc aacgacuucc accacgccca cgacgccuac cucaacgccg     3120

ucguggcguc cgcccugcug aagaaguacc ccaagcucga gcccgaguuc guguacggcg     3180

acuacccgaa guacaacagc uuccgggagc gcaaguccgc caccgagaag gucuacuucu     3240

acagcaacau caugaacauc uucaagaagu ccaucagccu ggccgacggg cgggugaucg     3300

agcgcccccu gaucgaggug aacgaggaga ccggcgaguc cgucuggaac aaggagagcg     3360

accucgcgac ggugcggcgc gugcuguccu acccccaggu caacguggug aagaaggucg     3420

aggagcagaa ccacgggcug gaccggggca agcccaaggg ccucuucaac gccaaccuga     3480

gcuccaagcc gaagcccaac agcaacgaga accugguggg ggccaaggag uaccucgacc     3540

ccaagaagua cggcggguac gccggcaucu ccaacagcuu caccgugcug gucaagggga     3600

ccaucgagaa gggcgccaag aagaagauca ccaacgugcu ggaguuccag ggcaucucca     3660

uccucgaccg caucaacuac cggaaggaca agcugaacuu ccugcucgag aagggguaca     3720

aggacaucga gcugaucauc gagcugccga aguacagccu cuucgagcug agcgacggcu     3780

cccgccgcau gcuggcgagc auccucucca cgaacaacaa gcgcggggag auccacaagg     3840

gcaaccagau cuuccugagc cagaaguucg ugaagcugcu cuaccacgcc aagcggaucu     3900

ccaacaccau caacgagaac caccggaagu acgucgagaa ccacaagaag gaguucgagg     3960

agcuguucua cuacauccug gaguucaacg agaacuacgu gggggccaag aagaacggca     4020

agcuccugaa cagcgccuuc caguccuggc agaaccacag caucgacgag cugugcucca     4080

gcuucaucgg ccccaccggg uccgagcgca agggccucuu cgagcugacc agccgggggu     4140

ccgccgcgga cuucgaguuc cugggcguga agaucccccg cuaccgggac uacacgccca     4200

gcucccuccu gaaggacgcc acccugaucc accagagcgu caccgggcuc uacgagaccc     4260

gcaucgaccu ggccaagcug ggcgagggca agcggccggc cgccacgaag aaggcggggc     4320

aggccaagaa gaagaaguaa ggacuaguaa uaaggaaacu guaugaaugu cugugggcag     4380

gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu     4440

uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4544


<210>  2448
<211>  3626
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2448
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc cgccuucaag cccaacagca ucaacuacau ccugggccuc gacaucggga      180

ucgccuccgu gggcugggcc augguggaga ucgacgagga ggagaacccc auccggcuga      240

ucgaccuggg gguccgcgug uucgagcggg cggaggugcc caagaccggc gacagccucg      300

ccauggcccg ccggcuggcc cgcuccgucc ggcgccugac ccggcgccgg gcgcaccgcc      360

uccugcggac gcgccggcug cucaagcgcg agggcgugcu gcaggccgcc aacuucgacg      420

agaacgggcu gaucaagagc cucccgaaca cccccuggca gcugcgggcc gccgcgcugg      480

accgcaagcu cacgccccug gagugguccg ccgugcugcu ccaccugauc aagcaccggg      540

gcuaccugag ccagcgcaag aacgaggggg agaccgccga caaggagcuc ggcgcccugc      600

ugaagggggu cgccggcaac gcgcacgccc uccagaccgg cgacuuccgg acccccgccg      660

agcuggcccu gaacaaguuc gagaaggagu ccgggcacau ccgcaaccag cgcagcgacu      720

acucccacac cuucagccgg aaggaccucc aggccgagcu gauccuccug uucgagaagc      780

agaaggaguu cggcaacccg cacguguccg ggggccugaa ggagggcauc gagacccugc      840

ucaugacgca gcgccccgcg cugagcgggg acgccgugca gaagaugcug ggccacugca      900

ccuucgagcc cgccgagccc aaggccgcca agaacaccua caccgcggag cgguucaucu      960

ggcucacgaa gcugaacaac cugcgcaucc ucgagcaggg guccgagcgg ccgcugaccg     1020

acaccgagcg cgccacccug auggacgagc ccuaccgcaa gagcaagcuc acguacgccc     1080

aggcccggaa gcugcugggc cucgaggaca ccgcguucuu caaggggcug cgguacggca     1140

aggacaacgc cgaggccucc acccugaugg agaugaaggc cuaccacgcc aucagccgcg     1200

cgcucgagaa ggagggccug aaggacaaga aguccccgcu gaaccugagc cccgagcucc     1260

aggacgagau cgggaccgcc uucucccugu ucaagacgga cgaggacauc accggccggc     1320

ugaaggaccg cauccagccg gagauccucg aggcccugcu gaagcacauc agcuucgaca     1380

aguucgucca gaucagccuc aaggcccugc ggcgcaucgu gccccugaug gagcagggga     1440

agcgguacga cgaggccugc gcggagaucu acggcgacca cuacgggaag aagaacaccg     1500

aggagaagau cuaccucccg cccauccccg ccgacgagau ccgcaacccc gugguccugc     1560

gggcccuguc ccaggcccgc aaggugauca acggcguggu ccggcgcuac ggcagccccg     1620

cccgcaucca caucgagacc gcgcgggagg uggggaaguc cuucaaggac cgcaaggaga     1680

ucgagaagcg gcaggaggag aaccgcaagg accgggagaa ggccgccgcc aaguuccgcg     1740

aguacuuccc caacuucgug ggcgagccca agagcaagga cauccucaag cugcggcugu     1800

acgagcagca gcacgggaag ugccucuacu ccggcaagga gaucaaccug gggcgccuga     1860

acgagaaggg cuacgucgag aucgaccacg cgcuccccuu cagccggacg ugggacgacu     1920

ccuucaacaa caaggugcug gugcugggca gcgagaacca gaacaagggg aaccagaccc     1980

ccuacgagua cuucaacggc aaggacaacu cccgcgagug gcaggaguuc aaggcccggg     2040

ucgagaccag ccgcuucccc cgguccaaga agcagcgcau ccuccugcag aaguucgacg     2100

aggacggguu caaggagcgg aaccugaacg acacccgcua cgugaaccgg uuccucugcc     2160

aguucgucgc cgaccgcaug cggcugacgg gcaagggcaa gaagcgcgug uucgccagca     2220

acgggcagau caccaaccug cuccggggcu ucugggggcu gcgcaaggug cgcgccgaga     2280

acgaccggca ccacgcgcug gacgccgucg ugguggccug cuccaccguc gccaugcagc     2340

agaagaucac ccgcuucgug cgguacaagg agaugaacgc cuucgacggc aagacgaucg     2400

acaaggagac cggggaggug cuccaccaga agacccacuu cccgcagccc ugggaguucu     2460

ucgcgcagga ggucaugauc cgcguguucg gcaagcccga cggcaagccg gaguucgagg     2520

aggccgacac ccuggagaag cugcggacgc uccuggccga gaagcugagc ucccgccccg     2580

aggccgugca cgaguacguc acgccccucu ucgugagccg ggcccccaac cgcaagaugu     2640

ccgggcaggg ccacauggag accgucaaga gcgcgaagcg gcuggacgag gggguguccg     2700

ugcugcgcgu gccgcucacc cagcugaagc ugaaggaccu cgagaagaug gucaaccggg     2760

agcgcgagcc caagcuguac gaggcccuga aggcccggcu cgaggcccac aaggacgacc     2820

ccgcgaaggc cuucgccgag cccuucuaca aguacgacaa ggccggcaac cgcacccagc     2880

aggucaaggc cgugcgggug gagcaggucc agaagaccgg ggugugggug cgcaaccaca     2940

acggcaucgc ggacaacgcc accauggugc ggguggacgu cuucgagaag ggcgacaagu     3000

acuaccuggu gccgaucuac agcuggcagg ucgccaaggg gauccugccc gaccgcgccg     3060

uggugcaggg caaggacgag gaggacuggc agcucaucga cgacagcuuc aacuucaagu     3120

ucucccugca ccccaacgac cugguggagg ucaucaccaa gaaggcccgg auguucgggu     3180

acuucgcgag cugccaccgc ggcaccggga acaucaacau ccgcauccac gaccucgacc     3240

acaagaucgg caagaacggc auccuggagg ggaucggcgu gaagacggcc cuguccuucc     3300

agaaguacca gaucgacgag cucgggaagg agauccggcc cugccgccug aagaagcggc     3360

cgcccgugcg caagcggccc gccgccacca agaaggccgg ccaggcgaag aagaagaagu     3420

aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa gagauccuuc     3480

uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau aauaaaucug     3540

guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa agaauu                                          3626


<210>  2449
<211>  4382
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2449
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gaagcccuac accaucggcc uggacaucgg gaccaacagc gugggcuggg      180

ccgugcucac ggaccaguac gaccugguca agcggaagau gaagaucgcc ggggacuccg      240

agaagaagca gaucaagaag aacuucuggg gcgugcgccu guucgacgag ggccagaccg      300

ccgccgaccg gcgcauggcg cggaccgccc gccggcgcau cgagcggcgc cggaaccgca      360

ucagcuaccu ccaggggauc uucgccgagg agauguccaa gaccgacgcc aacuucuucu      420

gccggcugag cgacuccuuc uacguggaca acgagaagcg caacagccgg caccccuucu      480

ucgcgacgau cgaggaggag gucgaguacc acaagaacua ccccaccauc uaccaccucc      540

gcgaggagcu ggugaacucc agcgagaagg ccgaccugcg gcugguguac cucgcccugg      600

cccacaucau caaguaccgc ggcaacuucc ugaucgaggg ggcccucgac acccagaaca      660

ccuccgucga cggcaucuac aagcaguuca uccagacgua caaccaggug uucgcgagcg      720

ggaucgagga cggcucccug aagaagcugg aggacaacaa ggacguggcc aagauccucg      780

ucgagaaggu gacccggaag gagaagcugg agcgcauccu gaagcucuac ccgggcgaga      840

aguccgccgg gauguucgcc caguucauca gccugaucgu gggcagcaag gggaacuucc      900

agaagcccuu cgaccugauc gagaaguccg acaucgagug cgccaaggac agcuacgagg      960

aggaccucga gucccugcug gcgcucaucg gcgacgagua cgccgagcug uucgucgccg     1020

ccaagaacgc cuacagcgcg guggugcugu ccagcaucau caccgucgcc gagaccgaga     1080

cgaacgccaa gcucagcgcc uccaugaucg agcgguucga cacccacgag gaggaccugg     1140

gcgagcugaa ggccuucauc aagcuccacc uccccaagca cuacgaggag aucuucagca     1200

acaccgagaa gcacggguac gcgggcuaca ucgacgggaa gaccaagcag gccgacuucu     1260

acaaguacau gaagaugacg cuggagaaca ucgagggcgc cgacuacuuc aucgccaaga     1320

ucgagaagga gaacuuccug cgcaagcagc gcaccuucga caacggggcg aucccccacc     1380

agcugcaccu ggaggagcuc gaggccaucc ugcaccagca ggccaaguac uacccguucc     1440

ugaaggagaa cuacgacaag aucaaguccc ucgugaccuu ccggaucccc uacuucgugg     1500

gcccccuggc caacggccag agcgaguucg ccuggcugac ccgcaaggcg gacggggaga     1560

uccggcccug gaacaucgag gagaaggucg acuucggcaa guccgccgug gacuucaucg     1620

agaagaugac gaacaaggac accuaccucc cgaaggagaa cgugcugccc aagcacagcc     1680

ugugcuacca gaaguaccuc gucuacaacg agcugaccaa ggugcgcuac aucaacgacc     1740

aggggaagac cuccuacuuc agcggccagg agaaggagca gaucuucaac gaccuguuca     1800

agcagaagcg gaaggucaag aagaaggacc ucgagcuguu ccugcgcaac augucccacg     1860

uggagagccc cacgaucgag gggcuggagg acuccuucaa cagcuccuac agcaccuacc     1920

acgaccuccu gaaggugggc aucaagcagg agauccugga caaccccguc aacaccgaga     1980

ugcucgagaa caucgugaag auccugaccg uguucgagga caagcggaug aucaaggagc     2040

agcugcagca guucuccgac guccucgacg gcguggugcu gaagaagcug gagcgccggc     2100

acuacacggg guggggccgc cucagcgcca agcugcugau ggggauccgg gacaagcagu     2160

cccaccucac cauccuggac uaccugauga acgacgacgg ccucaaccgc aaccugaugc     2220

agcugaucaa cgacagcaac cucuccuuca agagcaucau cgagaaggag caggucacca     2280

ccgccgacaa ggacauccag agcaucgugg ccgaccuggc gggguccccg gccaucaaga     2340

agggcauccu gcagagccuc aagaucgugg acgagcuggu cuccgugaug ggcuacccgc     2400

cccagacgau cguggucgag auggcccggg agaaccagac caccgggaag ggcaagaaca     2460

acagccgccc ccgguacaag ucccuggaga aggccaucaa ggaguucggg agccagaucc     2520

ucaaggagca ccccaccgac aaccaggagc ugcgcaacaa ccggcuguac cucuacuacc     2580

ugcagaacgg caaggacaug uacacgggcc aggaccugga cauccacaac cucuccaacu     2640

acgacaucga ccacaucgug ccccagagcu ucaucaccga caacuccauc gacaaccugg     2700

ugcugaccag cuccgccggg aaccgcgaga agggcgacga cgucccgccc cucgagaucg     2760

ugcggaagcg caagguguuc ugggagaagc uguaccaggg gaaccugaug agcaagcgca     2820

aguucgacua ccucaccaag gcggagcggg gcgggcugac cgaggccgac aaggcccgcu     2880

ucauccaccg gcagcugguc gagacgcgcc agaucaccaa gaacguggcc aacauccucc     2940

accagcgguu caacuacgag aaggacgacc acggcaacac caugaagcag guccgcaucg     3000

ugacccugaa guccgcgcug gugagccagu uccggaagca guuccagcuc uacaaggugc     3060

gcgacgucaa cgacuaccac cacgcccacg acgccuaccu gaacggcgug guggccaaca     3120

cgcugcucaa ggucuacccc cagcuggagc ccgaguucgu guacggggac uaccaccagu     3180

ucgacugguu caaggccaac aaggcgaccg ccaagaagca guucuacacc aacaucaugc     3240

uguucuucgc ccagaaggac cggaucaucg acgagaacgg cgagauccuc ugggacaaga     3300

aguaccugga caccgugaag aaggucaugu ccuaccgcca gaugaacauc gugaagaaga     3360

cggagaucca gaagggggag uucagcaagg ccaccaucaa gcccaagggc aacuccagca     3420

agcugauccc ccggaagacc aacugggacc cgaugaagua cgggggccuc gacagcccca     3480

acauggccua cgcggugguc aucgaguacg ccaagggcaa gaacaagcug guguucgaga     3540

agaagaucau ccgcgugacc aucauggagc ggaaggccuu cgagaaggac gagaaggccu     3600

uccuggagga gcagggguac cgccagccca agguccucgc caagcugccg aaguacacgc     3660

uguacgagug cgaggagggc cggcgccgga ugcucgcguc cgccaacgag gcccagaagg     3720

ggaaccagca gguccugccc aaccaccugg ugacccuccu gcaccacgcc gcgaacugcg     3780

aggugagcga cggcaagucc cuggacuaca ucgagagcaa ccgcgagaug uucgccgagc     3840

uccuggccca cguguccgag uucgccaagc gguacacccu ggccgaggcg aaccucaaca     3900

agaucaacca gcuguucgag cagaacaagg agggggacau caaggccauc gcccagagcu     3960

ucguggaccu gauggccuuc aacgccaugg gcgcgcccgc cuccuucaag uucuucgaga     4020

ccacgaucga gcgcaagcgg uacaacaacc ucaaggagcu gcugaacagc accaucaucu     4080

accaguccau caccggccuc uacgagagcc gcaagcgccu ggacgacaag cggcccgccg     4140

ccaccaagaa ggccgggcag gcgaagaaga agaaguaagg acuaguaaua aggaaacugu     4200

augaaugucu gugggcagga agugaagaga uccuucugua aagguuuuug gaauuauguc     4260

ugcugaauaa uaaacuuuuu ugaaauaaua aaucugguag aaaaaugaga ucuaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     4380

uu                                                                    4382


<210>  2450
<211>  4415
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2450
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gaagcccuac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccguggucac cgacgacuac aaggugcccg ccaagaagau gaaggugcuc gggaacacgg      240

acaagagcca caucgagaag aaccugcugg gcgcccuccu guucgacucc ggcaacaccg      300

ccgaggaccg gcgccugaag cggaccgcgc gccggcgcua cacccggcgc cggaaccgca      360

uccucuaccu gcaggagauc uucagcgagg agauggggaa ggucgacgac uccuucuucc      420

accggcugga ggacagcuuc cucgugacgg aggacaagcg cggcgagcgg caccccaucu      480

ucgggaaccu ggaggaggag gugaaguacc acgagaacuu cccgaccauc uaccaccucc      540

gccaguaccu ggccgacaac cccgagaagg ucgaccugcg gcugguguac cucgcccugg      600

cccacaucau caaguuccgc ggccacuucc ugaucgaggg gaaguucgac acccggaaca      660

acgacgugca gcgccucuuc caggaguucc uggcggucua cgacaacacc uucgagaacu      720

ccagccugca ggagcagaac guccaggugg aggagauccu cacggacaag aucuccaaga      780

gcgccaagaa ggaccgggug cugaagcugu uccccaacga gaaguccaac ggccgcuucg      840

ccgaguuccu caagcugauc gugggcaacc aggccgacuu caagaagcac uucgagcugg      900

aggagaaggc cccgcuccag uucagcaagg acaccuacga ggaggagcug gaggugcugc      960

ucgcgcagau cggggacaac uacgccgagc uguuccuguc cgccaagaag cucuacgaca     1020

gcauccugcu guccggcauc cucaccguca ccgacguggg gacgaaggcc ccgcugagcg     1080

ccuccaugau ccagcgguac aacgagcacc agauggaccu ggcgcagcuc aagcaguuca     1140

uccgccagaa gcugagcgac aaguacaacg agguguucuc cgacgucagc aaggacggcu     1200

acgccggcua caucgacggg aagaccaacc aggaggccuu cuacaaguac cugaagggcc     1260

uccugaacaa gaucgagggg agcggcuacu uccuggacaa gaucgagcgc gaggacuucc     1320

uccggaagca gcgcaccuuc gacaacgggu ccauccccca ccagauccac cuccaggaga     1380

ugcgggccau cauccgccgg caggccgagu ucuaccccuu ccuggcggac aaccaggacc     1440

gcaucgagaa gcugcugacc uuccggaucc ccuacuacgu gggcccgcug gcccgcggca     1500

agagcgacuu cgccuggcug ucccgcaaga gcgccgacaa gaucacgccc uggaacuucg     1560

acgagaucgu ggacaaggag uccagcgccg aggcguucau caaccggaug accaacuacg     1620

accucuaccu gcccaaccag aagguccugc ccaagcacuc ccuccuguac gagaaguuca     1680

ccguguacaa cgagcugacc aaggucaagu acaagacgga gcaggggaag accgccuucu     1740

ucgacgccaa caugaagcag gagaucuucg acggcguguu caagguguac cggaagguca     1800

ccaaggacaa gcucauggac uuccuggaga aggaguucga cgaguuccgc aucguggacc     1860

ugaccgggcu cgacaaggag aacaaggugu ucaacgccag cuacggcacg uaccacgacc     1920

ugugcaagau ccuggacaag gacuuccucg acaacuccaa gaacgagaag auccuggagg     1980

acaucguccu gacccucacc cuguucgagg accgggagau gauccgcaag cggcuggaga     2040

acuacagcga ccuccugacc aaggagcagg ucaagaagcu ggagcgccgg cacuacacgg     2100

gguggggccg ccucuccgcg gagcugaucc acggcauccg gaacaaggag agccgcaaga     2160

ccauccugga cuaccucauc gacgacggga acuccaaccg caacuucaug cagcugauca     2220

acgacgacgc ccugagcuuc aaggaggaga ucgccaaggc ccaggugauc ggcgagaccg     2280

acaaccucaa ccagguggug uccgacaucg ccgggagccc ggcgaucaag aagggcaucc     2340

ugcagucccu gaagaucgug gacgagcucg ucaagaucau ggggcaccag cccgagaaca     2400

ucguggugga gauggcccgg gagaaccagu ucaccaacca gggccgccgg aacagccagc     2460

agcgccugaa gggccugacg gacuccauca aggaguucgg gagccagauc cucaaggagc     2520

accccgucga gaacagccag cugcagaacg accggcuguu ccucuacuac cugcagaacg     2580

gccgcgacau guacaccggg gaggagcugg acaucgacua ccucucccag uacgacaucg     2640

accacaucau cccccaggcc uucaucaagg acaacagcau cgacaaccgg gugcugaccu     2700

ccagcaagga gaaccgcggc aaguccgacg acgugccgag caaggacguc gugcggaaga     2760

ugaaguccua cuggagcaag cugcucuccg ccaagcugau cacccagcgc aaguucgaca     2820

accugacgaa ggccgagcgg ggcgggcuca ccgacgacga caaggcgggc uucaucaagc     2880

gccagcuggu ggagacccgg cagaucacca agcacgucgc ccgcauccug gacgagcggu     2940

ucaacaccga gacggacgag aacaacaaga agauccgcca ggugaagauc gugacccuca     3000

agagcaaccu ggucuccaac uuccggaagg aguucgagcu guacaaggug cgcgagauca     3060

acgacuacca ccacgcccac gacgccuacc ucaacgccgu gaucgggaag gcgcugcugg     3120

gcgucuaccc ccagcucgag cccgaguucg uguacgggga cuacccccac uuccacggcc     3180

acaaggagaa caaggccacc gccaagaagu ucuucuacag caacaucaug aacuucuuca     3240

agaaggacga cgugcggacc gacaagaacg gcgagaucau cuggaagaag gacgagcaca     3300

ucuccaacau caagaagguc cugagcuacc cccaggugaa caucgugaag aaggucgagg     3360

agcagaccgg gggcuucucc aaggagagca uccugcccaa ggggaacucc gacaagcuca     3420

ucccccgcaa gacgaagaag uucuacuggg acaccaagaa guacggcggg uucgacagcc     3480

ccaucguggc cuacagcauc cuggugaucg cggacaucga gaagggcaag uccaagaagc     3540

ugaagaccgu caaggcccuc gugggcguga cgaucaugga gaagaugacc uucgagcggg     3600

acccggucgc cuuccuggag cgcaaggggu accgcaacgu gcaggaggag aacaucauca     3660

agcugcccaa guacagccuc uucaagcugg agaacggccg gaagcgccug cucgccuccg     3720

cccgggagcu gcagaagggg aacgagaucg ugcugcccaa ccaccucggc acccugcugu     3780

accacgcgaa gaacauccac aaggucgacg agccgaagca ccucgacuac guggacaagc     3840

acaaggacga guucaaggag cugcuggacg uggucagcaa cuucuccaag aaguacaccc     3900

ucgccgaggg gaaccuggag aagaucaagg agcuguacgc ccagaacaac ggcgaggacc     3960

ucaaggagcu ggccagcucc uucaucaacc ugcucacguu caccgccauc ggcgcgcccg     4020

ccaccuucaa guucuucgac aagaacaucg accgcaagcg guacaccagc acgaccgaga     4080

uccugaacgc cacccugauc caccagucca ucaccgggcu cuacgagacg cgcaucgacc     4140

ugaacaagcu gggcggggac aagcggcccg ccgccaccaa gaaggcgggc caggccaaga     4200

agaagaagua aggacuagua auaaggaaac uguaugaaug ucugugggca ggaagugaag     4260

agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua     4320

auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                4415


<210>  2451
<211>  3548
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2451
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgca gaccaccaac cugagcuaca uccucggccu ggaccugggg aucgccuccg      180

ugggcugggc cguggucgag aucaacgaga acgaggaccc caucgggcuc aucgacgugg      240

gcgugcggau cuucgagcgc gccgaggucc ccaagacggg cgagagccug gcccuguccc      300

ggcgccucgc gcggagcacc cgccggcuga uccgccggcg cgcccaccgg cugcuccugg      360

ccaagcgcuu ccugaagcgg gaggggaucc ucuccaccau cgaccuggag aagggccugc      420

ccaaccaggc cugggagcuc cgcguggcgg ggcuggagcg gcgccugagc gccaucgagu      480

ggggcgccgu gcuccugcac cugaucaagc accgggggua ccucuccaag cgcaagaacg      540

agagccagac caacaacaag gagcugggcg cccugcucuc cggcgucgcc cagaaccacc      600

agcugcugca gagcgacgac uaccggacgc cggcggagcu cgcccugaag aaguucgcca      660

aggaggaggg gcacauccgc aaccagcggg gcgccuacac ccacaccuuc aaccgccugg      720

accuccuggc cgagcugaac cuccuguucg cgcagcagca ccaguucggg aacccccacu      780

gcaaggagca cauccagcag uacaugaccg agcugcucau guggcagaag cccgcccugu      840

ccggcgaggc cauccugaag augcucggca agugcacgca cgagaagaac gaguucaagg      900

ccgccaagca caccuacagc gcggagcgcu ucguguggcu gaccaagcug aacaaccucc      960

ggauccugga ggacggggcc gagcgcgccc ugaacgagga ggagcggcag cuccugauca     1020

accaccccua cgagaagucc aagcugaccu acgcccaggu gcgcaagcuc cugggccuga     1080

gcgagcaggc caucuucaag caccuccggu acuccaagga gaacgcggag agcgccacgu     1140

ucauggagcu caaggccugg cacgccaucc gcaaggcgcu ggagaaccag gggcugaagg     1200

acaccuggca ggaccucgcc aagaagccgg accugcugga cgagaucggc accgccuucu     1260

cccucuacaa gaccgacgag gacauccagc aguaccugac gaacaagguc cccaacagcg     1320

ugaucaacgc ccugcucgug ucccugaacu ucgacaaguu caucgagcug agccucaaga     1380

gccugcggaa gauccugccc cucauggagc aggggaagcg cuacgaccag gccugccggg     1440

agaucuacgg ccaccacuac ggcgaggcga accagaagac cucccagcug cugcccgcca     1500

ucccggccca ggagauccgc aaccccgucg ugcuccggac ccugagccag gcccgcaagg     1560

ugaucaacgc caucauccgg caguacgggu cccccgcgcg cguccacauc gagaccggcc     1620

gggagcuggg caagagcuuc aaggagcgcc gggagaucca gaagcagcag gaggacaacc     1680

gcacgaagcg ggaguccgcc gugcagaagu ucaaggagcu guucagcgac uucuccagcg     1740

agcccaaguc caaggacauc cugaaguucc gccuguacga gcagcagcac gggaagugcc     1800

ucuacagcgg gaaggagauc aacauccacc gccugaacga gaagggcuac guggagaucg     1860

accacgcccu gccguucucc cggaccuggg acgacagcuu caacaacaag guccucgugc     1920

uggccuccga gaaccagaac aagggcaacc agacccccua cgaguggcug caggggaaga     1980

ucaacagcga gcgcuggaag aacuucgugg cccucguccu gggcucccag ugcagcgcgg     2040

ccaagaagca gcggcugcuc acccagguga ucgacgacaa caaguucauc gaccgcaacc     2100

ugaacgacac gcgguacauc gcccgcuucc uguccaacua cauccaggag aaccuccugc     2160

uggucgggaa gaacaagaag aacguguuca cccccaacgg ccagaucacc gcccuccugc     2220

ggagccgcug ggggcugauc aaggcgcggg agaacaacaa ccgccaccac gcccucgacg     2280

ccaucguggu cgccugcgcc acccccucca ugcagcagaa gaucacgcgg uucauccgcu     2340

ucaaggaggu gcacccguac aagaucgaga accgguacga gaugguggac caggagagcg     2400

gcgagaucau cagcccccac uuccccgagc ccugggcgua cuuccgccag gaggucaaca     2460

uccggguguu cgacaaccac ccggacaccg ugcugaagga gaugcugccc gaccgccccc     2520

aggccaacca ccaguucguc cagccccucu ucgugucccg ggccccgacc cgcaagauga     2580

gcggccaggg gcacauggag accaucaagu ccgccaagcg gcuggccgag ggcaucagcg     2640

ugcugcgcau cccccucacg cagcugaagc ccaaccugcu cgagaacaug gucaacaagg     2700

agcgggagcc ggcgcuguac gccgggcuga aggcccgccu cgccgaguuc aaccaggacc     2760

ccgccaaggc guucgccacc cccuucuaca agcagggcgg ccagcagguc aaggccaucc     2820

gcguggagca gguccagaag uccggggugc uggugcggga gaacaacggc guggccgaca     2880

acgcgagcau cgugcgcacc gacguguuca ucaagaacaa caaguucuuc cuggucccca     2940

ucuacaccug gcaggucgcc aaggggaucc ucccgaacaa ggccaucgug gcccacaaga     3000

acgaggacga gugggaggag auggacgagg gcgccaaguu caaguucucc cuguucccca     3060

acgaccuggu ggagcucaag acgaagaagg aguacuucuu cggguacuac aucggccugg     3120

accgggcgac cggcaacauc agccugaagg agcacgacgg ggagaucucc aagggcaagg     3180

acggggugua ccgcgugggc gucaagcucg cccugagcuu cgagaaguac cagguggacg     3240

agcuggggaa gaaccggcag aucugccgcc cccagcagcg gcagcccgug cgcaagcggc     3300

cggccgccac caagaaggcc ggccaggcga agaagaagaa guaaggacua guaauaagga     3360

aacuguauga augucugugg gcaggaagug aagagauccu ucuguaaagg uuuuuggaau     3420

uaugucugcu gaauaauaaa cuuuuuugaa auaauaaauc ugguagaaaa augagaucua     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaagaauu                                                              3548


<210>  2452
<211>  3626
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2452
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc cgccuucaag cccaacccca ucaacuacau ccugggccuc gacaucggga      180

ucgccagcgu gggcugggcc augguggaga ucgacgagga cgagaacccc aucugccuga      240

ucgaccuggg gguccgggug uucgagcgcg cggaggugcc gaagaccggc gacucccucg      300

ccauggcccg gcgccuggcc cggagcgucc gccggcugac ccgccggcgc gcgcaccggc      360

uccugcgcgc ccggcgccug cucaagcggg agggcgugcu gcaggccgcc gacuucgacg      420

agaacgggcu gaucaagucc cuccccaaca cgcccuggca gcugcgcgcc gcggcccugg      480

accggaagcu cacgccccug gaguggagcg ccgugcugcu ccaccugauc aagcaccgcg      540

gcuaccuguc ccagcggaag aacgaggggg agaccgccga caaggagcuc ggcgcccugc      600

ugaagggggu cgcggacaac gcccacgccc uccagaccgg cgacuuccgc accccggccg      660

agcuggcccu gaacaaguuc gagaaggaga gcggccacau ccggaaccag cgcggggacu      720

acucccacac cuucagccgc aaggaccucc aggcggagcu gauccuccuc uucgagaagc      780

agaaggaguu cggcaacccc cacguguccg ggggccugaa ggagggcauc gagacccugc      840

ucaugaccca gcggcccgcc cugagcgggg acgccgugca gaagaugcug ggccacugca      900

cguucgagcc cgccgagccg aaggccgcga agaacaccua caccgccgag cgcuucaucu      960

ggcucaccaa gcugaacaac cugcggaucc ucgagcaggg guccgagcgc ccccugacgg     1020

acaccgagcg ggccacccug auggacgagc ccuaccgcaa gagcaagcuc accuacgccc     1080

aggcgcggaa gcugcugggc cucgaggaca cggccuucuu caaggggcug cgcuacggca     1140

aggacaacgc cgaggccucc acccugaugg agaugaaggc cuaccacgcg aucagccggg     1200

cccucgagaa ggagggccug aaggacaaga aguccccgcu gaaccugagc cccgagcucc     1260

aggacgagau cgggaccgcc uucucccugu ucaagaccga cgaggacauc acgggccgcc     1320

ugaaggaccg gauccagccc gagauccucg aggcccugcu gaagcacauc agcuucgaca     1380

aguucgucca gaucucccuc aaggcccugc gccggaucgu gccccugaug gagcagggga     1440

agcgcuacga cgaggcgugc gccgagaucu acggcgacca cuacgggaag aagaacaccg     1500

aggagaagau cuaccugccc ccgauccccg ccgacgagau ccggaacccc gugguccugc     1560

gcgcccugag ccaggcccgg aaggugauca acggcguggu ccgccgguac ggcagccccg     1620

cgcgcaucca caucgagacc gcccgcgagg uggggaaguc cuucaaggac cggaaggaga     1680

ucgagaagcg ccaggaggag aaccggaagg accgcgagaa ggccgccgcg aaguuccggg     1740

aguacuuccc gaacuucgug ggcgagccca agagcaagga cauccucaag cugcgccugu     1800

acgagcagca gcacgggaag ugccucuacu ccggcaagga gaucaaccug gggcggcuga     1860

acgagaaggg cuacgucgag aucgaccacg cccuccccuu cagccgcacc ugggacgacu     1920

ccuucaacaa caaggugcug gugcugggca gcgagaacca gaacaagggg aaccagacgc     1980

ccuacgagua cuucaacggc aaggacaacu cccgggagug gcaggaguuc aaggcccgcg     2040

ucgagaccag ccgguucccg cgcuccaaga agcagcggau ccuccugcag aaguucgacg     2100

aggacggguu caaggagcgc aaccugaacg acacccggua cgugaaccgc uuccucugcc     2160

aguucgucgc cgaccggaug cgccugaccg gcaagggcaa gaagcgggug uucgccagca     2220

acgggcagau cacgaaccug cuccgcggcu ucugggggcu gcggaaggug cgcgcggaga     2280

acgaccgcca ccacgcccug gacgccgucg ugguggccug cuccaccguc gccaugcagc     2340

agaagaucac ccgguucgug cgcuacaagg agaugaacgc guucgacggc aagaccaucg     2400

acaaggagac gggggaggug cuccaccaga agacccacuu cccccagccc ugggaguucu     2460

ucgcccagga ggucaugauc cggguguucg gcaagccgga cggcaagccc gaguucgagg     2520

aggccgacac ccccgagaag cugcgcaccc ugcucgccga gaagcugagc ucccggcccg     2580

aggccgugca cgaguacguc acgccgcugu ucgugagccg cgcgcccaac cggaagaugu     2640

ccgggcaggg ccacauggag accgucaaga gcgccaagcg ccucgacgag gggguguccg     2700

ugcugcgggu gccccugacc cagcucaagc ugaaggaccu ggagaagaug gucaaccgcg     2760

agcgggagcc caagcucuac gaggcccuga aggcccgccu ggaggcgcac aaggacgacc     2820

cggccaaggc cuucgccgag cccuucuaca aguacgacaa ggccggcaac cggacccagc     2880

aggucaaggc ggugcgcgug gagcaggucc agaagaccgg ggugugggug cggaaccaca     2940

acggcaucgc cgacaacgcc acgauggugc gcguggacgu cuucgagaag ggcgacaagu     3000

acuaccucgu gcccaucuac agcuggcagg ucgccaaggg gauccugccc gaccgggccg     3060

uggugcaggg caaggacgag gaggacuggc agcugaucga cgacuccuuc aacuucaagu     3120

ucagccucca cccgaacgac cugguggagg ucaucaccaa gaaggcgcgc auguucgggu     3180

acuucgccag cugccaccgg ggcaccggga acaucaacau ccgcauccac gaccuggacc     3240

acaagaucgg caagaacggc auccucgagg ggaucggcgu gaagaccgcc cuguccuucc     3300

agaaguacca gaucgacgag cuggggaagg agauccgccc cugccggcuc aagaagcgcc     3360

cgcccgugcg gaagcgcccc gccgccacga agaaggcggg ccaggccaag aagaagaagu     3420

aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa gagauccuuc     3480

uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau aauaaaucug     3540

guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa agaauu                                          3626


<210>  2453
<211>  3632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2453
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa guaccacgug ggcaucgacg uggggaccuu cagcgucggc cuggccgcca      180

ucgaggugga cgacgccggg augcccauca agacccuguc ccuggugagc cacauccacg      240

acuccggccu ggaccccgac gagaucaaga gcgccgucac gcggcucgcg uccagcggca      300

ucgcccgccg gacccgccgg cuguaccgcc ggaagcgccg gcgccugcag cagcucgaca      360

aguucaucca gcggcagggg uggcccguga ucgagcugga ggacuacucc gacccgcugu      420

accccuggaa ggugcgcgcc gagcucgccg cgagcuacau cgccgacgag aaggagcggg      480

gcgagaagcu guccgucgcc cugcgccaca ucgcccggca ccgcgggugg cggaaccccu      540

acgccaaggu gagcucccuc uaccugcccg acggcccgag cgacgcguuc aaggccaucc      600

gcgaggagau caagcgggcc uccgggcagc ccgugcccga gaccgccacc gucggccaga      660

uggugacgcu gugcgagcuc ggcacccuga agcugcgcgg ggagggcggg gugcucagcg      720

cccggcugca gcaguccgac uacgcgcgcg agauccagga gaucugccgc augcaggaga      780

ucggccagga gcuguaccgg aagaucaucg acgucguguu cgccgccgag agccccaagg      840

gcuccgccag cucccgcgug gggaaggacc cgcuccagcc cggcaagaac cgggcccuga      900

aggcgagcga cgccuuccag cgcuaccgga ucgccgcccu gaucgggaac cuccgcgucc      960

ggguggacgg cgagaagcgc auccugagcg uggaggagaa gaaccugguc uucgaccacc     1020

ucgugaaccu gacccccaag aaggagcccg agugggugac caucgccgag auccugggga     1080

ucgaccgggg ccagcucauc ggcacggcga ccaugaccga cgacggggag cgcgccggcg     1140

cccggccgcc cacccacgac acgaaccgcu ccaucgucaa cagccggauc gcgccccugg     1200

uggacuggug gaagaccgcc uccgcccugg agcagcacgc cauggucaag gcccucagca     1260

acgccgaggu ggacgacuuc gacucccccg agggggcgaa ggugcaggcc uucuucgccg     1320

accuggacga cgacguccac gccaagcugg acagccucca ccucccggug ggccgcgccg     1380

cguacuccga ggacacccug gugcggcuca cccgccggau gcugagcgac ggggucgacc     1440

uguacacggc ccgccuccag gaguucggca ucgagcccuc cuggaccccg cccacgcccc     1500

ggaucggcga gcccgugggg aaccccgccg uggaccgcgu ccugaagacc gugagccgcu     1560

ggcuggaguc cgccaccaag accuggggcg cccccgagcg ggugaucauc gagcacgucc     1620

gcgagggguu cgugaccgag aagcgggcgc gcgagaugga cggcgacaug cggcgccggg     1680

ccgcccgcaa cgccaagcug uuccaggaga ugcaggagaa gcugaacgug caggggaagc     1740

cgagccgggc ggaccugugg cgcuaccagu ccguccagcg gcagaacugc cagugcgccu     1800

acugcggcag ccccaucacc uucuccaaca gcgagaugga ccacaucgug ccccgcgccg     1860

gccagggguc cacgaacacc cgggagaacc ucguggccgu cugccaccgc ugcaaccaga     1920

gcaagggcaa caccccguuc gccaucuggg cgaagaacac cuccaucgag ggggugagcg     1980

ugaaggaggc cgucgagcgg acgcgccacu gggugaccga caccggcaug cggagcaccg     2040

acuucaagaa guucacgaag gccguggucg agcgcuucca gcgggccacc auggacgagg     2100

agaucgacgc ccgcuccaug gagagcgugg cguggauggc caacgagcug cggucccgcg     2160

uggcccagca cuucgccagc cacggcacca ccguccgcgu guaccggggg ucccugacgg     2220

ccgaggcgcg ccgggccagc ggcaucuccg ggaagcugaa guucuucgac ggcgugggca     2280

agagccgccu cgaccggcgc caccacgcca ucgacgccgc ggucaucgcc uucaccuccg     2340

acuacguggc cgagacccug gccgugcgga gcaaccugaa gcagucccag gcccaccgcc     2400

aggaggcgcc ccaguggcgg gaguucaccg ggaaggacgc cgagcaccgc gccgccuggc     2460

gggucuggug ccagaagaug gagaagcuca gcgcccugcu gaccgaggac cuccgcgacg     2520

accggguggu ggucaugucc aacgugcgcc ugcggcuggg caacgggagc gcgcacaagg     2580

agacgaucgg caagcucucc aaggugaagc ugagcuccca gcugagcguc agcgacaucg     2640

acaaggccuc cagcgaggcc cucuggugcg cccugacccg cgagcccggg uucgacccca     2700

aggagggccu gccggccaac cccgagcggc acauccgcgu gaacgggacc cacguguacg     2760

cgggcgacaa caucggccuc uuccccgucu ccgccgggag caucgcccug cggggcgggu     2820

acgccgagcu gggcuccagc uuccaccacg cccgcgugua caagaucacc uccgggaaga     2880

agcccgcguu cgccaugcuc cgcguguaca cgaucgaccu gcugccguac cggaaccagg     2940

accucuucag cgucgagcug aagccccaga ccauguccau gcgccaggcc gagaagaagc     3000

ugcgggacgc ccucgcgacc ggcaacgccg aguaccuggg cuggcuggug guggacgacg     3060

agcucgucgu ggacaccagc aagaucgcca cggaccaggu gaaggccguc gaggccgagc     3120

uggggaccau ccgccggugg cgcguggacg gcuucuucuc ccccagcaag cugcggcucc     3180

gcccccugca gauguccaag gaggggauca agaaggagag cgcgccggag cuguccaaga     3240

ucaucgaccg gcccggcugg cuccccgccg ucaacaagcu guucagcgac gggaacguga     3300

ccgugguccg ccgggacucc cugggccgcg ugcggcucga gagcaccgcc caccuccccg     3360

ugacguggaa gguccagaag cgcccggccg ccaccaagaa ggcgggccag gccaagaaga     3420

agaaguaagg acuaguaaua aggaaacugu augaaugucu gugggcagga agugaagaga     3480

uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua     3540

aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3632


<210>  2454
<211>  3332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2454
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc ccggauccug gccuucgaca ucggcaucag cuccaucggg ugggccuuca      180

gcgagaacga cgagcucaag gacugcggcg ugcgcaucuu caccaaggug gagaacccca      240

agaccgggga gucccuggcc cugccccggc gccucgcgcg gagcgcccgc aagcggcugg      300

cccgccggaa ggcccgccug aaccaccuca agcaccugau cgcgaacgag uucaagcuga      360

acuacgagga cuaccagucc uucgacgaga gccucgccaa ggccuacaag ggcucccuga      420

ucagccccua cgagcugcgg uuccgcgccc ucaacgagcu gcuguccaag caggacuucg      480

cccgggucau ccuccacauc gcgaagcgcc ggggcuacga cgacaucaag aacagcgacg      540

acaaggagaa gggggccauc cugaaggcca ucaagcagaa cgaggagaag cuggccaacu      600

accaguccgu gggcgaguac cucuacaagg aguacuucca gaaguucaag gagaacagca      660

aggaguucac gaacgugcgc aacaagaagg aguccuacga gcggugcauc gcccagagcu      720

uccugaagga cgagcugaag cucaucuuca agaagcagcg cgaguucggg uucuccuuca      780

gcaagaaguu cgaggaggag guccuguccg uggcguucua caagcgggcc cugaaggacu      840

ucagccaccu cgugggcaac ugcuccuucu ucaccgacga gaagcgcgcc ccgaagaaca      900

gcccccuggc cuucauguuc gucgcccuga cccggaucau caaccuccug aacaaccuga      960

agaacaccga ggggauccuc uacacgaagg acgaccugaa cgcgcugcuc aacgaggugc     1020

ugaagaacgg cacccugacc uacaagcaga ccaagaagcu ccugggccug agcgacgacu     1080

acgaguucaa gggggagaag ggcacguacu ucaucgaguu caagaaguac aaggaguuca     1140

ucaaggcccu cggggagcac aaccuguccc aggacgaccu gaacgagauc gccaaggaca     1200

ucacccucau caaggacgag aucaagcuga agaaggcccu ggccaaguac gaccucaacc     1260

agaaccagau cgacagccug uccaagcugg aguucaagga ccaccucaac aucagcuuca     1320

aggcgcugaa gcuggugacg ccccugaugc ucgagggcaa gaaguacgac gaggccugca     1380

acgagcugaa ccugaagguc gccaucaacg aggacaagaa ggacuuccuc cccgccuuca     1440

acgagaccua cuacaaggac gaggugacca acccgguggu ccugcgcgcg aucaaggagu     1500

accgcaaggu gcugaacgcc cuccugaaga aguacggcaa ggugcacaag aucaacaucg     1560

agcuggcccg ggaggucggg aagaaccacu cccagcgcgc caagaucgag aaggagcaga     1620

acgagaacua caaggccaag aaggacgcgg agcucgagug cgagaagcug ggccugaaga     1680

ucaacagcaa gaacauccuc aagcugcggc uguucaagga gcagaaggag uucugcgccu     1740

acuccgggga gaagaucaag aucagcgacc uccaggacga gaagaugcug gagaucgacc     1800

acaucuaccc cuacucccgc agcuucgacg acuccuacau gaacaaggug cugguguuca     1860

ccaagcagaa ccaggagaag cucaaccaga cccccuucga ggccuucggc aacgacagcg     1920

ccaaguggca gaagaucgag guccuggcca agaaccugcc caccaagaag cagaagcgga     1980

uccucgacaa gaacuacaag gacaaggagc agaagaacuu caaggaccgc aaccugaacg     2040

acacgcggua caucgcgcgc cuggugcuca acuacaccaa ggacuaccug gacuuccugc     2100

cgcucuccga cgacgagaac accaagcuga acgacaccca gaaggggagc aaggugcacg     2160

ucgaggccaa guccggcaug cugacgagcg cccuccggca caccuggggc uucuccgcca     2220

aggaccgcaa caaccaccuc caccacgcca ucgacgcggu gaucaucgcc uacgccaaca     2280

acagcaucgu caaggccuuc uccgacuuca agaaggagca ggagagcaac agcgcggagc     2340

uguacgccaa gaagaucucc gagcuggacu acaagaacaa gcggaaguuc uucgagcccu     2400

ucagcggguu ccgccagaag gugcuggaca agaucgacga gaucuucgug uccaagcccg     2460

agcggaagaa gcccagcggc gcccugcacg aggagaccuu ccgcaaggag gaggaguucu     2520

accaguccua cgggggcaag gagggggucc ucaaggcccu ggagcugggc aagauccgga     2580

aggugaacgg caagaucgug aagaacgggg acauguuccg cgucgacauc uucaagcaca     2640

agaagaccaa caaguucuac gccgugccga ucuacacgau ggacuucgcg cucaaggugc     2700

ugcccaacaa ggccgucgcc cggagcaaga agggcgagau caaggacugg auccugaugg     2760

acgagaacua cgaguucugc uucucccucu acaaggacag ccugauccug auccagacca     2820

aggacaugca ggagcccgag uucguguacu acaacgccuu caccuccagc accguguccc     2880

ucaucgucag caagcacgac aacaaguucg agacgcuguc caagaaccag aagauccugu     2940

ucaagaacgc caacgagaag gaggugaucg ccaagagcau cgggauccag aaccucaagg     3000

uguucgagaa guacaucguc uccgcgcugg gcgaggugac caaggccgag uuccgccagc     3060

gggaggacuu caagaagaag cgccccgccg ccaccaagaa ggcggggcag gccaagaaga     3120

agaaguaagg acuaguaaua aggaaacugu augaaugucu gugggcagga agugaagaga     3180

uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua     3240

aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3300

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3332


<210>  2455
<211>  3743
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2455
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgag cgaccuggug cucggccugg acaucgggau cggcuccgug ggggucggca      180

uccugaacaa ggugaccggc gagaucaucc acaagaacag ccggaucuuc cccgccgccc      240

aggccgagaa caaccucgug cgccggacca accgccaggg gcggcgccug gcccggcgca      300

agaagcaccg gcgcguccgg cugaaccgcc ucuucgagga guccggccug aucacggacu      360

ucaccaagau cagcaucaac cugaaccccu accagcuccg ggugaagggg cugaccgacg      420

agcuguccaa cgaggagcug uucaucgcgc ugaagaacau ggugaagcac cgcggcauca      480

gcuaccugga cgacgccucc gacgacggga acagcuccgu cggcgacuac gcccagaucg      540

ugaaggagaa cagcaagcag cucgagacca agacgcccgg ccagauccag cuggagcggu      600

accagaccua cgggcagcug cgcggcgacu ucaccgugga gaaggacggg aagaagcacc      660

ggcucaucaa cgucuucccg accuccgccu accgcagcga ggcgcugcgg auccugcaga      720

cgcagcagga guucaacccc cagaucaccg acgaguucau caaccgcuac cucgagaucc      780

ugaccggcaa gcggaaguac uaccacggcc ccgggaacga gaagucccgc accgacuacg      840

gccgcuaccg gacgagcggg gagacccugg acaacaucuu cggcauccuc aucgggaagu      900

gcaccuucua ccccgacgag uuccgcgccg ccaaggccuc cuacaccgcc caggaguuca      960

accugcugaa cgaccucaac aaccugacgg ugccgaccga gaccaagaag cugagcaagg     1020

agcagaagaa ccagaucauc aacuacguga agaacgagaa ggcgaugggc cccgccaagc     1080

uguucaagua caucgccaag cuccuguccu gcgacgucgc cgacaucaag ggcuaccgga     1140

ucgacaagag cgggaaggcc gagauccaca ccuucgaggc guaccgcaag augaagacgc     1200

ucgagacccu ggacaucgag cagauggacc gggagacccu ggacaagcuc gccuacgugc     1260

ugacccugaa cacggagcgc gagggcaucc aggaggcccu cgagcacgag uucgccgacg     1320

gguccuucag ccagaagcag gucgacgagc uggugcaguu ccggaaggcc aacagcucca     1380

ucuucggcaa gggguggcac aacuucagcg ugaagcugau gauggagcuc auccccgagc     1440

uguacgagac cuccgaggag cagaugacca uccugacccg ccucggcaag cagaagacga     1500

ccagcuccag caacaagacc aaguacaucg acgagaagcu gcugaccgag gagaucuaca     1560

accccguggu cgcgaagucc gugcggcagg ccaucaagau cgugaacgcc gccaucaagg     1620

aguacggcga cuucgacaac aucgucaucg agauggcccg cgagacgaac gaggacgacg     1680

agaagaaggc gauccagaag auccagaagg ccaacaagga cgagaaggac gccgccaugc     1740

ucaaggcggc caaccaguac aacgggaagg ccgagcugcc gcacagcgug uuccacggcc     1800

acaagcagcu ggccaccaag auccggcugu ggcaccagca gggggagcgc ugccucuaca     1860

ccggcaagac caucuccauc cacgaccuga ucaacaacag caaccaguuc gaggucgacc     1920

acauccugcc ccucuccauc acguucgacg acagccuggc caacaaggug cugguguacg     1980

cgaccgccaa ccaggagaag gggcagcgga cccccuacca ggcccucgac uccauggacg     2040

acgccuggag cuuccgcgag cugaaggccu ucguccggga guccaagacc cugagcaaca     2100

agaagaagga guaccuccug accgaggagg acaucuccaa guucgacgug cgcaagaagu     2160

ucaucgagcg gaaccuggug gacacgcgcu acgcgagccg ggucgugcuc aacgcccugc     2220

aggagcacuu ccgcgcccac aagaucgaca ccaagguguc cgucgugcgc ggccaguuca     2280

ccagccagcu gcggcgccac uggggcaucg agaagacccg ggacacguac caccaccacg     2340

ccguggacgc ccucaucauc gcggccagcu cccagcugaa ccuguggaag aagcagaaga     2400

acacccucgu cagcuacucc gaggaccagc ugcuggacau cgagaccggg gagcucauca     2460

gcgacgacga guacaaggag uccguguuca aggcccccua ccagcacuuc guggacaccc     2520

ucaagagcaa ggaguucgag gacuccaucc uguucagcua ccaggucgac uccaaguuca     2580

accgcaagau cagcgacgcc acgaucuacg cgacccggca ggccaaggug ggcaaggaca     2640

aggccgacga gaccuacgug cucgggaaga ucaaggacau cuacacccag gacggcuacg     2700

acgccuucau gaagaucuac aagaaggaca aguccaaguu ccugauguac cgccacgacc     2760

cgcagacguu cgagaagguc aucgagccca uccuggagaa cuaccccaac aagcagauca     2820

acgagaaggg caaggaggug cccugcaacc cguuccucaa guacaaggag gagcacgggu     2880

acauccggaa guacagcaag aagggcaacg ggcccgagau caagucccug aaguacuacg     2940

acagcaagcu gggcaaccac aucgacauca cccccaagga cuccaacaac aagguggucc     3000

uccagagcgu gucccccugg cgcgccgacg uguacuucaa caagaccacc gggaaguacg     3060

agauccuggg ccugaaguac gcggaccucc aguucgagaa gggcacgggg accuacaaga     3120

ucagccagga gaaguacaac gacaucaaga agaaggaggg cgucgacagc gacuccgagu     3180

ucaaguucac ccuguacaag aacgaccugc uccuggugaa ggacaccgag acgaaggagc     3240

agcagcuguu ccgguuccuc agccgcacca ugccgaagca gaagcacuac guggagcuga     3300

agcccuacga caagcagaag uucgagggcg gggaggcccu gaucaagguc cucgggaacg     3360

uggccaacuc cggccagugc aagaagggcc ugggcaagag caacaucucc aucuacaagg     3420

ugcggaccga cguccugggg aaccagcaca ucaucaagaa cgagggggac aagcccaagc     3480

ucgacuucaa gcgccccgcc gccaccaaga aggcgggcca ggccaagaag aagaaguaag     3540

gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag auccuucugu     3600

aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau aaaucuggua     3660

gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaga auu                                             3743


<210>  2456
<211>  4301
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2456
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgac ccaguucgag ggcuucacca accuguacca ggugagcaag acgcuccggu      180

ucgagcugau cccccagggg aagacccuga agcacaucca ggagcagggc uucaucgagg      240

aggacaaggc ccgcaacgac cacuacaagg agcucaagcc caucaucgac cggaucuaca      300

agaccuacgc cgaccagugc cugcagcugg ugcagcucga cugggagaac cuguccgccg      360

ccaucgacag cuaccgcaag gagaagaccg aggagacgcg gaacgcgcug aucgaggagc      420

aggccaccua ccgcaacgcc auccacgacu acuucaucgg gcggaccgac aaccucaccg      480

acgccaucaa caagcgccac gcggagaucu acaagggccu guucaaggcc gagcuguuca      540

acggcaaggu ccucaagcag cuggggacgg ugaccaccac cgagcacgag aacgcccugc      600

uccgguccuu cgacaaguuc acgaccuacu ucagcggcuu cuacgagaac cgcaagaacg      660

uguucuccgc cgaggacauc agcaccgcca ucccccaccg gaucguccag gacaacuucc      720

cgaaguucaa ggagaacugc cacaucuuca cccgccugau cacggcggug cccucccugc      780

gggagcacuu cgagaacgug aagaaggcca ucgggaucuu cgucagcacc uccaucgagg      840

agguguucag cuuccccuuc uacaaccagc uccugaccca gacccagauc gaccuguaca      900

accagcuccu gggcgggauc ucccgcgagg ccggcacgga gaagaucaag ggccugaacg      960

aggugcucaa ccuggccauc cagaagaacg acgagaccgc ccacaucauc gcgagccugc     1020

cccaccgguu caucccccuc uucaagcaga uccuguccga ccgcaacacc cugagcuuca     1080

uccucgagga guucaagucc gacgaggagg ucauccagag cuucugcaag uacaagaccc     1140

ugcugcggaa cgagaacgug cucgagacgg ccgaggcccu guucaacgag cugaacucca     1200

ucgaccucac ccacaucuuc aucagccaca agaagcugga gaccaucucc agcgcccugu     1260

gcgaccacug ggacacccuc cgcaacgccc uguacgagcg gcgcaucagc gagcugacgg     1320

ggaagaucac caaguccgcg aaggagaagg ugcagcggag ccucaagcac gaggacauca     1380

accugcagga gaucaucucc gccgccggca aggagcugag cgaggccuuc aagcagaaga     1440

ccuccgagau ccucagccac gcccacgcgg cccuggacca gccgcugccc accacgcuca     1500

agaagcagga ggagaaggag auccugaagu cccagcugga cagccugcuc gggcuguacc     1560

accuccucga cugguucgcc gucgacgagu ccaacgaggu ggaccccgag uucagcgccc     1620

gccugaccgg caucaagcug gagauggagc cgucccucag cuucuacaac aaggcgcgca     1680

acuacgccac caagaagccc uacuccgugg agaaguucaa gcugaacuuc cagaugccca     1740

cccuggccag cggcugggac gucaacaagg agaagaacaa cggggccauc cucuucguga     1800

agaacggccu guacuaccug gggaucaugc ccaagcagaa gggccgguac aaggcccucu     1860

ccuucgagcc gacggagaag accagcgagg gguucgacaa gauguacuac gacuacuucc     1920

ccgacgcggc caagaugauc cccaagugcu ccacccagcu gaaggccgug accgcccacu     1980

uccagaccca cacgaccccc auccugcuca gcaacaacuu caucgagccg cuggagauca     2040

ccaaggagau cuacgaccug aacaaccccg agaaggagcc caagaaguuc cagaccgccu     2100

acgcgaagaa gacgggcgac cagaagggcu accgcgaggc ccucugcaag uggaucgacu     2160

ucacccggga cuuccugucc aaguacacca agaccacgag caucgaccug agcucccucc     2220

gccccagcuc ccaguacaag gaccuggggg aguacuacgc cgagcugaac ccgcuccugu     2280

accacaucag cuuccagcgg aucgccgaga aggagaucau ggacgccguc gagaccggca     2340

agcuguaccu cuuccagauc uacaacaagg acuucgcgaa ggggcaccac ggcaagccca     2400

accugcacac ccuguacugg accggccucu ucucccccga gaaccuggcc aagacgagca     2460

ucaagcugaa cgggcaggcc gagcuguucu accgccccaa gucccggaug aagcgcaugg     2520

cccaccggcu gggcgagaag augcugaaca agaagcucaa ggaccagaag accccgaucc     2580

ccgacacccu guaccaggag cuguacgacu acgugaacca ccgccucagc cacgaccugu     2640

ccgacgaggc gcgggcccug cuccccaacg ugaucaccaa ggaggucagc cacgagauca     2700

ucaaggaccg ccgguucacg uccgacaagu ucuucuucca cgugccgauc acccugaacu     2760

accaggccgc caacagcccc uccaaguuca accagcgcgu caacgccuac cugaaggagc     2820

accccgagac ccccaucauc gggaucgacc ggggcgagcg caaccucauc uacaucaccg     2880

ugaucgacag cacggggaag auccuggagc agcggucccu gaacaccauc cagcaguucg     2940

acuaccagaa gaagcucgac aaccgcgaga aggagcgggu ggcggcccgc caggccugga     3000

gcgucguggg caccaucaag gaccugaagc agggguaccu gucccaggug auccacgaga     3060

ucgucgaccu caugauccac uaccaggccg uggugguccu ggagaaccug aacuucggcu     3120

ucaagagcaa gcgcaccggc aucgccgaga aggcggugua ccagcaguuc gagaagaugc     3180

ucaucgacaa gcugaacugc cuggugcuca aggacuaccc ggccgagaag gucgggggcg     3240

ugcugaaccc cuaccagcug acggaccagu ucaccagcuu cgccaagaug gggacccagu     3300

ccggcuuccu cuucuacgug cccgcccccu acaccagcaa gaucgacccg cugacgggcu     3360

ucgucgaccc cuucgugugg aagaccauca agaaccacga gucccggaag cacuuccugg     3420

agggguucga cuuccuccac uacgacguga agaccggcga cuucauccug cacuucaaga     3480

ugaaccgcaa ccugagcuuc cagcgggggc uccccggcuu caugcccgcc ugggacaucg     3540

ucuucgagaa gaacgagacc caguucgacg cgaaggggac gccguucauc gccggcaagc     3600

gcaucgugcc cgugaucgag aaccaccggu ucaccggccg cuaccgggac cuguaccccg     3660

ccaacgagcu gaucgcccuc cuggaggaga aggggaucgu cuuccgcgac ggcuccaaca     3720

uccugcccaa gcuccuggag aacgacgaca gccacgcgau cgacaccaug guggcccuga     3780

uccgguccgu gcuccagaug cgcaacagca acgccgccac cggggaggac uacaucaacu     3840

ccccgguccg ggaccugaac ggcgugugcu ucgacagccg cuuccagaac cccgaguggc     3900

ccauggacgc cgacgcgaac ggggccuacc acaucgcccu gaagggccag cuccugcuga     3960

accaccucaa ggaguccaag gaccugaagc ugcagaacgg caucagcaac caggacuggc     4020

ucgccuacau ccaggagcug cggaacaagc gccccgccgc gacgaagaag gccgggcagg     4080

ccaagaagaa gaaguaagga cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa     4140

gugaagagau ccuucuguaa agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu     4200

gaaauaauaa aucugguaga aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         4301


<210>  2457
<211>  4280
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2457
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgag caucuaccag gaguucguga acaaguacuc ccugagcaag acccuccggu      180

ucgagcugau cccccagggc aagacccugg agaacaucaa ggcccgcggg cucauccugg      240

acgacgagaa gcgggccaag gacuacaaga aggccaagca gaucaucgac aaguaccacc      300

aguucuucau cgaggagauc cuguccagcg ugugcaucuc cgaggaccuc cugcagaacu      360

acagcgacgu cuacuucaag cugaagaagu ccgacgacga caaccuccag aaggacuuca      420

agagcgccaa ggacacgauc aagaagcaga ucuccgagua caucaaggac agcgagaagu      480

ucaagaaccu guucaaccag aaccugaucg acgcgaagaa gggccaggag uccgaccuca      540

uccuguggcu gaagcagagc aaggacaacg ggaucgagcu guucaaggcc aacuccgaca      600

ucaccgacau cgacgaggcc cucgagauca ucaagagcuu caagggcugg accaccuacu      660

ucaagggcuu ccacgagaac cgcaagaacg uguacuccag caacgacauc cccacgucca      720

ucaucuaccg gaucguggac gacaaccugc ccaaguuccu cgagaacaag gccaaguacg      780

agagccugaa ggacaaggcg ccggaggcca ucaacuacga gcagaucaag aaggaccugg      840

ccgaggagcu caccuucgac aucgacuaca agaccuccga ggucaaccag cgcguguuca      900

gccuggacga gguguucgag aucgccaacu ucaacaacua ccugaaccag agcgggauca      960

ccaaguucaa cacgaucauc ggcgggaagu ucgucaacgg cgagaacacc aagcggaagg     1020

ggaucaacga guacaucaac cucuacuccc agcagaucaa cgacaagacc cugaagaagu     1080

acaagaugag cgugcuguuc aagcagaucc ucuccgacac cgagagcaag uccuucguga     1140

ucgacaagcu ggaggacgac agcgacgucg ugacgaccau gcaguccuuc uacgagcaga     1200

ucgccgcguu caagaccgug gaggagaagu ccaucaagga gacccugucc cuccuguucg     1260

acgaccugaa ggcccagaag cucgaccuga gcaagaucua cuucaagaac gacaaguccc     1320

ugacggaccu cagccagcag gucuucgacg acuacuccgu gaucggcacc gccgugcugg     1380

aguacaucac ccagcagauc gcccccaaga accuggacaa ccccagcaag aaggagcagg     1440

agcucaucgc caagaagacc gagaaggcga aguaccuguc ccuggagacg aucaagcucg     1500

cccuggagga guucaacaag caccgcgaca ucgacaagca gugccgguuc gaggagaucc     1560

uggccaacuu cgccgccauc cccaugaucu ucgacgagau cgcgcagaac aaggacaacc     1620

ucgcccagau cagcaucaag uaccagaacc agggcaagaa ggaccugcug caggccuccg     1680

ccgaggacga cgucaaggcc aucaaggacc uccuggacca gaccaacaac cugcugcaca     1740

agcucaagau cuuccacauc agccaguccg aggacaaggc gaacauccug gacaaggacg     1800

agcacuucua ccugguguuc gaggagugcu acuucgagcu cgccaacauc gugccgcugu     1860

acaacaagau ccgcaacuac aucacccaga agcccuacag cgacgagaag uucaagcuga     1920

acuucgagaa cagcacccuc gccaacgggu gggacaagaa caaggagccc gacaacacgg     1980

ccauccuguu caucaaggac gacaaguacu accugggcgu caugaacaag aagaacaaca     2040

agaucuucga cgacaaggcg aucaaggaga acaaggggga gggcuacaag aagaucgugu     2100

acaagcuccu gcccggcgcc aacaagaugc ugccgaaggu guucuucucc gccaagagca     2160

ucaaguucua caaccccucc gaggacaucc uccggauccg caaccacagc acccacacca     2220

agaacgggag cccccagaag ggcuacgaga aguucgaguu caacaucgag gacugccgga     2280

aguucaucga cuucuacaag cagagcaucu ccaagcaccc cgaguggaag gacuucgggu     2340

uccgcuucag cgacacccag cgguacaacu ccaucgacga guucuaccgc gaggucgaga     2400

accagggcua caagcugacg uucgagaaca ucagcgaguc cuacaucgac agcgugguca     2460

accaggggaa gcuguaccuc uuccagaucu acaacaagga cuucuccgcc uacagcaagg     2520

gccggccgaa ccugcacacc cuguacugga aggcccucuu cgacgagcgc aaccugcagg     2580

acguggugua caagcugaac ggcgaggcgg agcuguucua ccggaagcag uccaucccca     2640

agaagaucac ccaccccgcc aaggaggcca ucgccaacaa gaacaaggac aaccccaaga     2700

aggagagcgu cuucgaguac gaccucauca aggacaagcg cuucaccgag gacaaguucu     2760

ucuuccacug cccgaucacg aucaacuuca aguccagcgg ggccaacaag uucaacgacg     2820

agaucaaccu gcuccugaag gagaaggcga acgacgugca cauccugagc aucgaccggg     2880

gcgagcgcca ccucgccuac uacacccugg uggacgggaa gggcaacauc aucaagcagg     2940

acaccuucaa caucaucggg aacgaccgca ugaagaccaa cuaccacgac aagcuggccg     3000

ccaucgagaa ggaccgggac uccgcccgca aggacuggaa gaagaucaac aacaucaagg     3060

agaugaagga gggcuaccuc agccaggucg ugcacgagau cgcgaagcug gugaucgagu     3120

acaacgccau cgucguguuc gaggaccuga acuucggcuu caagcggggg cgcuucaagg     3180

uggagaagca ggucuaccag aagcucgaga agaugcugau cgagaagcug aacuaccucg     3240

uguucaagga caacgaguuc gacaagacgg gcggggugcu gcgggccuac cagcugaccg     3300

cccccuucga gaccuucaag aagaugggca agcagaccgg gaucaucuac uacguccccg     3360

cgggcuucac cuccaagauc ugccccguga cgggcuucgu gaaccagcuc uacccgaagu     3420

acgagagcgu cuccaagagc caggaguucu ucuccaaguu cgacaagauc ugcuacaacc     3480

uggacaaggg guacuucgag uucagcuucg acuacaagaa cuucggcgac aaggccgcca     3540

aggggaagug gaccaucgcc uccuucggca gccgccugau caacuuccgg aacuccgaca     3600

agaaccacaa cugggacacc cgcgaggugu accccaccaa ggagcucgag aagcugcuga     3660

aggacuacag caucgaguac ggccacgggg agugcaucaa ggccgcgauc ugcggcgagu     3720

ccgacaagaa guucuucgcc aagcucacga gcgugcugaa caccauccug cagaugcgga     3780

acuccaagac cgggaccgag cucgacuacc ugaucagccc cgucgccgac gugaacggca     3840

acuucuucga cucccgccag gcccccaaga acaugccgca ggacgccgac gcgaacgggg     3900

ccuaccacau cggccugaag ggccucaugc ugcuggggcg gaucaagaac aaccaggagg     3960

gcaagaagcu caaccuggug aucaagaacg aggaguacuu cgaguucguc cagaaccgca     4020

acaacaagcg gcccgccgcc acgaagaagg ccgggcaggc gaagaagaag aaguaaggac     4080

uaguaauaag gaaacuguau gaaugucugu gggcaggaag ugaagagauc cuucuguaaa     4140

gguuuuugga auuaugucug cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa     4200

aaaugagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaagaauu                                                 4280


<210>  2458
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2458
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggacaa gaaguacagc      240

aucggccugg acaucgggac caacuccgug ggcugggccg ugaucaccga cgaguacaag      300

guccccagca agaaguucaa ggugcugggg aacacggacc ggcacuccau caagaagaac      360

cugaucggcg cccugcuguu cgacagcggc gagaccgccg aggccacccg ccugaagcgg      420

accgcgcgcc ggcgcuacac gcggcgcaag aaccggaucu gcuaccugca ggagaucuuc      480

uccaacgaga uggccaaggu ggacgacagc uucuuccacc gccucgagga guccuuccug      540

gucgaggagg acaagaagca cgagcggcac cccaucuucg ggaacaucgu ggacgaggug      600

gccuaccacg agaaguaccc caccaucuac caccuccgca agaagcucgu cgacagcacc      660

gacaaggccg accugcggcu gaucuaccuc gcgcuggccc acaugaucaa guuccgcggc      720

cacuuccuga ucgaggggga ccucaacccg gacaacuccg acguggacaa gcuguucauc      780

cagcuggugc agaccuacaa ccagcuguuc gaggagaacc ccaucaacgc cagcggcguc      840

gacgccaagg ccauccuguc cgcgcggcug agcaaguccc gccggcucga gaaccugauc      900

gcccagcugc ccggggagaa gaagaacggc cucuucggca accugaucgc ccugagccuc      960

gggcugacgc ccaacuucaa guccaacuuc gaccuggccg aggacgccaa gcuccagcug     1020

agcaaggaca ccuacgacga cgaccuggac aaccuccugg cgcagaucgg cgaccaguac     1080

gccgaccugu uccucgccgc caagaaccug uccgacgcca uccugcucag cgacauccug     1140

cgcgugaaca ccgagaucac caaggcgccg cuguccgcca gcaugaucaa gcgguacgac     1200

gagcaccacc aggaccucac gcugcugaag gcccucgugc gccagcagcu gcccgagaag     1260

uacaaggaga ucuucuucga ccagagcaag aacggguacg ccggcuacau cgacggcggg     1320

gccucccagg aggaguucua caaguucauc aagcccaucc uggagaagau ggacggcacc     1380

gaggagcucc uggucaagcu gaaccgggag gaccugcucc gcaagcagcg caccuucgac     1440

aacgggagca ucccccacca gauccaccug ggcgagcugc acgcgauccu ccggcgccag     1500

gaggacuucu acccguuccu gaaggacaac cgggagaaga ucgagaagau ccugaccuuc     1560

cgcauccccu acuacguggg gccccucgcc cggggcaacu cccgcuucgc cuggaugacg     1620

cgcaagagcg aggagaccau cacccccugg aacuucgagg agguggucga caagggcgcc     1680

uccgcgcaga gcuucaucga gcggaugacc aacuucgaca agaaccugcc gaacgagaag     1740

gugcugccca agcacucccu ccuguacgag uacuucacgg uguacaacga gcugaccaag     1800

gucaaguacg ugaccgaggg gaugcggaag cccgccuucc ucagcggcga gcagaagaag     1860

gccaucgugg accugcuguu caagaccaac cgcaagguca cggugaagca gcucaaggag     1920

gacuacuuca agaagaucga gugcuucgac uccgucgaga ucagcggggu ggaggaccgg     1980

uucaacgccu cccugggcac cuaccacgac cugcucaaga ucaucaagga caaggacuuc     2040

cuggacaacg aggagaacga ggacauccug gaggacaucg ugcucacccu gacccuguuc     2100

gaggaccgcg agaugaucga ggagcggcuc aagacguacg cccaccuguu cgacgacaag     2160

gucaugaagc agcugaagcg ccggcgcuac accggguggg gccggcucag ccgcaagcug     2220

aucaacggca uccgggacaa gcaguccggg aagaccaucc uggacuuccu caagagcgac     2280

ggcuucgcga accgcaacuu caugcagcug auccacgacg acucccugac cuucaaggag     2340

gacauccaga aggcccaggu gagcgggcag ggcgacuccc uccacgagca caucgccaac     2400

cuggccggga gccccgccau caagaagggc auccugcaga cggugaaggu cguggacgag     2460

cucgugaagg ucaugggccg ccacaagccg gagaacaucg ugaucgagau ggcgcgggag     2520

aaccagacca cccagaaggg gcagaagaac ucccgcgagc ggaugaagcg caucgaggag     2580

ggcaucaagg agcuggggag ccagauccug aaggagcacc ccguggagaa cacccagcuc     2640

cagaacgaga agcuguaccu guacuaccuc cagaacggcc gggacaugua cgucgaccag     2700

gagcuggaca ucaaccgccu gagcgacuac gacguggacc acaucgugcc ccaguccuuc     2760

cucaaggacg acagcaucga caacaagguc cugacgcggu ccgacaagaa ccgcggcaag     2820

agcgacaacg ugcccuccga ggaggugguc aagaagauga agaacuacug gcggcagcug     2880

cucaacgcca agcugaucac ccagcgcaag uucgacaacc ugaccaaggc cgagcgcggg     2940

ggccucagcg agcuggacaa ggccggguuc aucaagcggc agcuggugga gacccggcag     3000

aucaccaagc acguggccca gauccucgac ucccgcauga acacgaagua cgacgagaac     3060

gacaagcuga uccgggaggu caaggugauc acccucaaga gcaagcucgu guccgacuuc     3120

cgcaaggacu uccaguucua caagguccgg gagaucaaca acuaccacca cgcgcacgac     3180

gccuaccuga acgccguggu gggcaccgcc cugaucaaga aguacccgaa gcucgagagc     3240

gaguucgucu acggggacua caagguguac gacgugcgca agaugaucgc gaaguccgag     3300

caggagaucg gcaaggccac cgccaaguac uucuucuaca gcaacaucau gaacuucuuc     3360

aagacggaga ucacccuggc caacggcgag auccggaagc gcccccugau cgagaccaac     3420

ggggagaccg gcgagaucgu cugggacaag gggcgggacu ucgccacggu gcgcaaggug     3480

cucuccaugc cccaggucaa caucgugaag aagaccgagg ugcagaccgg cggguucagc     3540

aaggagucca uccugcccaa gcgcaacagc gacaagcuga ucgcgcggaa gaaggacugg     3600

gacccgaaga aguacggcgg cuucgacucc cccaccgucg ccuacagcgu gcucgugguc     3660

gccaaggugg agaagggcaa gagcaagaag cugaaguccg ugaaggagcu gcucgggauc     3720

acgaucaugg agcgcagcuc cuucgagaag aaccccaucg acuuccugga ggccaagggg     3780

uacaaggagg ucaagaagga ccugaucauc aagcucccga aguacagccu guucgagcug     3840

gagaacggcc ggaagcgcau gcucgccucc gcgggggagc ugcagaaggg caacgagcug     3900

gcccucccca gcaaguacgu gaacuuccug uaccuggccu cccacuacga gaagcucaag     3960

ggcagccccg aggacaacga gcagaagcag cuguucgugg agcagcacaa gcacuaccug     4020

gacgagauca ucgagcagau cuccgaguuc agcaagcggg ucauccucgc cgacgccaac     4080

cuggacaagg ugcuguccgc guacaacaag caccgcgaca agcccauccg ggagcaggcc     4140

gagaacauca uccaccucuu cacccugacc aaccuggggg ccccggccgc guucaaguac     4200

uucgacacca cgaucgaccg caagcgguac accagcacca aggaggugcu cgacgccacc     4260

cugauccacc aguccaucac gggccuguac gagacccgca ucgaccucag ccagcucggg     4320

ggcgacaagc ggcccgccgc caccaagaag gccgggcagg cgaagaagaa gaaguaagga     4380

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     4440

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     4500

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaagaau u                                               4581


<210>  2459
<211>  5364
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2459
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaacuu caagauccug      240

cccaucgcca ucgaccucgg cgugaagaac accggggugu ucagcgccuu cuaccagaag      300

ggcaccuccc uggagcggcu ggacaacaag aacgggaagg ucuacgagcu cagcaaggac      360

uccuacacgc ugcugaugaa caaccgcacc gcccggcgcc accagcggcg cggcaucgac      420

cggaagcagc ucgugaagcg ccuguucaag cugaucugga ccgagcagcu caaccuggag      480

ugggacaagg acacccagca ggccaucagc uuccuguuca accggcgcgg cuucuccuuc      540

aucacggacg gguacagccc cgaguaccuc aacaucgugc ccgagcaggu caaggcgauc      600

cugauggaca ucuucgacga cuacaacggc gaggacgacc uggacuccua ccucaagcug      660

gccaccgagc aggagagcaa gaucuccgag aucuacaaca agcugaugca gaagauccuc      720

gaguucaagc ugaugaagcu gugcaccgac aucaaggacg acaaggugag caccaagacg      780

cucaaggaga ucaccuccua cgaguucgag cugcuggccg acuaccucgc caacuacagc      840

gagucccuga agacccagaa guucagcuac accgacaagc aggggaaccu gaaggagcuc      900

uccuacuacc accacgacaa guacaacauc caggaguucc ugaagcggca cgcgacgauc      960

aacgaccgca uccuggacac ccuccugacc gacgaccugg acaucuggaa cuucaacuuc     1020

gagaaguucg acuucgacaa gaacgaggag aagcuccaga accaggagga caaggaccac     1080

auccaggccc accuccacca cuucguguuc gccgucaaca agaucaagag cgagauggcc     1140

uccggcgggc ggcaccgcag ccaguacuuc caggagauca ccaacgugcu ggacgagaac     1200

aaccaccagg agggcuaccu gaagaacuuc ugcgagaacc ugcacaacaa gaaguacucc     1260

aaccugagcg ugaagaaccu cgucaaccug aucggcaacc ugagcaaccu cgagcugaag     1320

ccgcugcgga aguacuucaa cgacaagauc cacgccaagg cggaccacug ggacgagcag     1380

aaguucacgg agaccuacug ccacuggauc cucggggagu ggcgcguggg cgugaaggac     1440

caggacaaga aggacggggc caaguacucc uacaaggacc ugugcaacga gcugaagcag     1500

aaggucacca aggccggccu cguggacuuc cugcuggagc uggaccccug ccggaccauc     1560

ccgcccuacc ucgacaacaa caaccgcaag ccgcccaagu gccagagccu gauccugaac     1620

cccaaguucc ucgacaacca guaccccaac uggcagcagu accugcagga gcugaagaag     1680

cuccagucca uccagaacua ccuggacagc uucgagacgg accugaaggu gcucaagucc     1740

agcaaggacc agcccuacuu cgucgaguac aaguccagca accagcagau cgccuccggc     1800

cagcgggacu acaaggaccu ggacgcccgc auccugcagu ucaucuucga ccgggugaag     1860

gcgagcgacg agcuccugcu gaacgagauc uacuuccagg ccaagaagcu caagcagaag     1920

gccuccagcg agcuggagaa gcuggagucc agcaagaagc ucgacgaggu gaucgccaac     1980

ucccagcuga gccagauccu gaagucccag cacaccaacg ggaucuucga gcagggcacc     2040

uuccuccacc uggucugcaa guacuacaag cagcgccagc gcgcccggga cagccgccug     2100

uacaucaugc ccgaguaccg guacgacaag aagcuccaca aguacaacaa caccgggcgc     2160

uucgacgacg acaaccagcu gcugacguac ugcaaccaca agccccggca gaagcgcuac     2220

cagcuccuga acgaccuggc gggcgugcuc cagguguccc ccaacuuccu gaaggacaag     2280

aucgggagcg acgacgaccu guucaucagc aaguggcucg ucgagcacau ccggggcuuc     2340

aagaaggccu gcgaggacuc ccugaagauc cagaaggaca accgcggccu gcucaaccac     2400

aagaucaaca ucgcccggaa caccaagggg aagugcgaga aggagaucuu caaccugauc     2460

ugcaagaucg agggcagcga ggacaagaag gggaacuaca agcacggccu ggccuacgag     2520

cucggggugc ugcuguucgg cgagccgaac gaggccucca agcccgaguu cgaccgcaag     2580

aucaagaagu ucaacagcau cuacuccuuc gcgcagaucc agcagaucgc cuucgccgag     2640

cggaagggca acgccaacac gugcgcgguc ugcagcgccg acaacgccca ccgcaugcag     2700

cagaucaaga ucaccgagcc cguggaggac aacaaggaca agaucauccu cuccgccaag     2760

gcccagcggc ugcccgcgau cccgacccgc aucguggacg gggccgucaa gaagauggcc     2820

accauccugg ccaagaacau cguggacgac aacuggcaga acaucaagca gguccucagc     2880

gccaagcacc agcugcacau ccccaucauc accgagucca acgcguucga guucgagccc     2940

gcccuggccg acgugaaggg caagagccuc aaggaccggc gcaagaaggc ccuggagcgg     3000

aucucccccg agaacaucuu caaggacaag aacaaccgca ucaaggaguu cgccaagggg     3060

aucagcgcgu acuccggcgc caaccugacc gacggggacu ucgacggcgc caaggaggag     3120

cucgaccaca ucaucccgcg gagccacaag aaguacggca cgcugaacga cgaggccaac     3180

cugaucugcg ugacccgcgg ggacaacaag aacaagggca accgcaucuu cugccuccgg     3240

gaccuggcgg acaacuacaa gcugaagcag uucgagacca ccgacgaccu cgagaucgag     3300

aagaagaucg ccgacacgau cugggacgcc aacaagaagg acuucaaguu cgggaacuac     3360

cgcuccuuca ucaaccugac gccccaggag cagaaggccu uccggcacgc ccuguuccuc     3420

gcggacgaga accccaucaa gcaggccgug auccgcgcca ucaacaaccg gaaccgcacc     3480

uucgucaacg gcacccagcg guacuucgcc gaggugcugg ccaacaacau cuaccugcgc     3540

gcgaagaagg agaaccucaa caccgacaag aucagcuucg acuacuucgg cauccccacc     3600

aucgggaacg gccgggggau cgccgagauc cgccagcugu acgagaaggu ggacuccgac     3660

auccaggccu acgccaaggg cgacaagccg caggccagcu acagccaccu gaucgacgcg     3720

augcucgccu ucugcaucgc cgccgacgag caccggaacg acggguccau cggccuggag     3780

aucgacaaga acuacagccu guacccccuc gacaagaaca ccggcgaggu cuucaccaag     3840

gacaucuucu cccagaucaa gaucaccgac aacgaguuca gcgacaagaa gcuggugcgc     3900

aagaaggcga ucgagggguu caacacgcac cggcagauga cccgcgacgg caucuacgcc     3960

gagaacuacc ugcccauccu cauccacaag gagcugaacg aggugcggaa gggguacacc     4020

uggaagaacu ccgaggagau caagaucuuc aagggcaaga aguacgacau ccagcagcug     4080

aacaaccucg ucuacugccu gaaguucgug gacaagccga ucagcaucga cauccagauc     4140

uccacccugg aggagcuccg caacauccug acgaccaaca acaucgccgc caccgccgag     4200

uacuacuaca ucaaccugaa gacccagaag cuccacgagu acuacaucga gaacuacaac     4260

acggcgcugg gguacaagaa guacagcaag gagauggagu uccugcgguc ccucgccuac     4320

cgcagcgagc gggugaagau caaguccauc gacgacguca agcagguccu ggacaaggac     4380

agcaacuuca ucaucggcaa gaucacccug cccuucaaga aggaguggca gcgccucuac     4440

cgggaguggc agaacaccac caucaaggac gacuacgagu uccugaaguc cuucuucaac     4500

gucaagagca ucacgaagcu gcacaagaag gugcgcaagg acuucucccu ccccaucagc     4560

accaacgagg gcaaguuccu ggugaagcgc aagaccuggg acaacaacuu caucuaccag     4620

auccugaacg acuccgacag ccgggccgac gggaccaagc ccuucauccc ggccuucgac     4680

aucuccaaga acgagaucgu ggaggccauc aucgacagcu ucacgagcaa gaacaucuuc     4740

uggcucccca agaacaucga gcugcagaag guggacaaca agaacaucuu cgcgaucgac     4800

accuccaagu gguucgaggu ggagaccccc agcgaccugc gcgacaucgg caucgccacc     4860

auccaguaca agaucgacaa caacucccgg cccaaggugc gcgucaagcu ggacuacgug     4920

aucgacgacg acagcaagau caacuacuuc augaaccacu cccuccucaa gagccgguac     4980

ccggacaagg ugcuggagau ccugaagcag uccacgauca ucgaguucga gagcuccggg     5040

uucaacaaga ccaucaagga gaugcugggc augaagcugg ccgggaucua caacgagacc     5100

agcaacaaca agcgccccgc cgccaccaag aaggcgggcc aggccaagaa gaagaaguaa     5160

ggacuaguaa uaaggaaacu guaugaaugu cugugggcag gaagugaaga gauccuucug     5220

uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu uuugaaauaa uaaaucuggu     5280

agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5340

aaaaaaaaaa aaaaaaaaag aauu                                            5364


<210>  2460
<211>  3636
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2460
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagcg gaacuacauc      240

cugggccucg acaucgggau caccagcgug ggcuacggga ucaucgacua cgagacccgc      300

gacgugaucg acgccggcgu ccggcuguuc aaggaggcca acguggagaa caacgagggc      360

cgccggucca agcgcggggc ccggcgccug aagcggcgcc ggcgccaccg gauccagcgc      420

gugaagaagc uccuguucga cuacaaccug cucacggacc acagcgagcu guccggcauc      480

aaccccuacg aggcccgggu caaggggcug agccagaagc ucuccgagga ggaguucagc      540

gcggcccugc ugcaccucgc caagcgccgg ggcgugcaca acgugaacga ggucgaggag      600

gacaccggga acgagcuguc caccaaggag cagaucagcc gcaacuccaa ggcccuggag      660

gagaaguacg uggcggagcu ccagcuggag cggcugaaga aggacggcga ggugcgcggc      720

agcaucaacc gguucaagac cuccgacuac gucaaggagg ccaagcagcu ccugaaggug      780

cagaaggccu accaccagcu ggaccagagc uucaucgaca cguacaucga ccuccuggag      840

acccgccgca ccuacuacga ggggcccggc gagggguccc ccuucggcug gaaggacauc      900

aaggaguggu acgagaugcu gaugggccac ugcaccuacu ucccggagga gcuccggagc      960

gugaaguacg ccuacaacgc cgaccuguac aacgcgcuga acgaccucaa caaccugguc     1020

aucacgcgcg acgagaacga gaagcuggag uacuacgaga aguuccagau caucgagaac     1080

guguucaagc agaagaagaa gcccacccuc aagcagaucg ccaaggagau ccuggugaac     1140

gaggaggaca ucaaggggua ccgggucacc uccaccggca agcccgaguu cacgaaccug     1200

aagguguacc acgacaucaa ggacaucacc gcccgcaagg agaucaucga gaacgccgag     1260

cuccuggacc agaucgccaa gauccugacc aucuaccaga gcuccgagga cauccaggag     1320

gagcucacca accugaacag cgagcugacg caggaggaga ucgagcagau cuccaaccuc     1380

aagggguaca ccggcaccca caaccugagc cugaaggcga ucaaccucau ccuggacgag     1440

cuguggcaca ccaacgacaa ccagaucgcc aucuucaacc ggcucaagcu ggugcccaag     1500

aaggucgacc ugagccagca gaaggagauc ccgacgaccc ucguggacga cuucauccug     1560

ucccccgugg ucaagcgcag cuucauccag uccaucaagg ugaucaacgc caucaucaag     1620

aaguacgggc ugcccaacga caucaucauc gagcuggccc gggagaagaa cagcaaggac     1680

gcccagaaga ugaucaacga gaugcagaag cgcaaccggc agaccaacga gcgcaucgag     1740

gagaucaucc ggaccacggg caaggagaac gcgaaguacc ucaucgagaa gaucaagcug     1800

cacgacaugc aggagggcaa gugccuguac ucccucgagg ccaucccccu ggaggaccug     1860

cucaacaacc cguucaacua cgaggucgac cacaucaucc cccgcagcgu guccuucgac     1920

aacagcuuca acaacaaggu gcuggucaag caggaggaga acuccaagaa ggggaaccgg     1980

acccccuucc aguaccugag cuccagcgac uccaagauca gcuacgagac cuucaagaag     2040

cacauccuca accuggccaa gggcaagggg cgcaucucca agaccaagaa ggaguaccug     2100

cucgaggagc gggacaucaa ccgcuucagc gugcagaagg acuucaucaa ccggaaccug     2160

guggacacgc gcuacgccac ccggggccug augaaccucc ugcgcuccua cuuccgcguc     2220

aacaaccugg acgugaaggu caagagcauc aacgggggcu ucaccuccuu ccuccggcgc     2280

aaguggaagu ucaagaagga gcggaacaag ggcuacaagc accacgccga ggacgcgcug     2340

aucaucgcca acgccgacuu caucuucaag gaguggaaga agcuggacaa ggccaagaag     2400

gugauggaga accagauguu cgaggagaag caggcggaga gcaugcccga gaucgagacc     2460

gagcaggagu acaaggagau cuucaucacg ccgcaccaga ucaagcacau caaggacuuc     2520

aaggacuaca aguacagcca ccgcguggac aagaagccca accgggagcu caucaacgac     2580

acccuguacu ccacccgcaa ggacgacaag gggaacaccc ugaucgugaa caaccucaac     2640

ggccuguacg acaaggacaa cgacaagcug aagaagcuca ucaacaagag ccccgagaag     2700

cugcugaugu accaccacga cccccagacg uaccagaagc ucaagcugau cauggagcag     2760

uacggggacg agaagaaccc gcuguacaag uacuacgagg agaccggcaa cuaccucacc     2820

aaguacucca agaaggacaa cgggcccguc aucaagaaga ucaaguacua cggcaacaag     2880

cugaacgccc accuggacau caccgacgac uaccccaaca gccggaacaa gguggugaag     2940

cucucccuga agcccuaccg cuucgacguc uaccuggaca acggcgugua caaguucgug     3000

accgucaaga accucgacgu gaucaagaag gagaacuacu acgaggugaa cagcaagugc     3060

uacgaggagg ccaagaagcu gaagaagauc uccaaccagg ccgaguucau cgccagcuuc     3120

uacaacaacg accugaucaa gaucaacggg gagcucuacc gggucaucgg cgugaacaac     3180

gaccugcuga accgcaucga ggugaacaug aucgacauca cguaccggga guaccucgag     3240

aacaugaacg acaagcgccc gccccggauc aucaagacca ucgcguccaa gacccagagc     3300

aucaagaagu acuccaccga cauccugggg aaccuguacg aggucaagag caagaagcac     3360

ccccagauca ucaagaaggg caagcgcccc gccgccacga agaaggccgg ccaggccaag     3420

aagaagaagu aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     3480

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     3540

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3636


<210>  2461
<211>  4704
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2461
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgcuguu caacaagugc      240

aucaucauca gcaucaaccu cgacuucucc aacaaggaga agugcaugac caagcccuac      300

agcaucggcc uggacaucgg gaccaacucc gugggcuggg ccgugaucac ggacaacuac      360

aaggucccca gcaagaagau gaaggugcug gggaacaccu ccaagaagua caucaagaag      420

aaccuccugg gcgugcugcu guucgacagc ggcaucaccg ccgaggggcg gcgccugaag      480

cggaccgccc gccggcgcua cacgcggcgc cggaaccgca uccuguaccu ccaggagauc      540

uucuccaccg agauggccac ccuggacgac gcguucuucc agcggcugga cgacagcuuc      600

cucguccccg acgacaagcg cgacuccaag uacccgaucu ucggcaaccu gguggaggag      660

aagguguacc acgacgaguu ccccaccauc uaccaccucc ggaaguaccu cgccgacagc      720

acgaagaagg ccgaccugcg ccuggucuac cucgcccugg cgcacaugau caaguaccgg      780

gggcacuucc ugaucgaggg cgaguucaac uccaagaaca acgacaucca gaagaacuuc      840

caggacuucc ucgacaccua caacgccauc uucgagagcg accugucccu ggagaacagc      900

aagcagcucg aggagaucgu gaaggacaag aucuccaagc uggagaagaa ggaccgcauc      960

cugaagcugu uccccgggga gaagaacagc ggcaucuucu ccgaguuccu gaagcugauc     1020

gugggcaacc aggccgacuu ccggaagugc uucaaccucg acgagaaggc cagccugcac     1080

uucuccaagg agagcuacga cgaggaccug gagacccucc ugggguacau cggcgacgac     1140

uacagcgacg ucuuccugaa ggccaagaag cucuacgacg cgauccugcu guccggguuc     1200

cucaccguga cggacaacga gaccgaggcg ccccugagcu ccgccaugau caagcgcuac     1260

aacgagcaca aggaggaccu ggcccuccug aaggaguaca uccggaacau cagccugaag     1320

accuacaacg agguguucaa ggacgacacc aagaacggcu acgccggcua caucgacggg     1380

aagacgaacc aggaggacuu cuacgucuac cucaagaacc ugcuggccga guucgagggc     1440

gccgacuacu uccucgagaa gaucgaccgc gaggacuucc ugcggaagca gcgcaccuuc     1500

gacaacgggu ccaucccgua ccagauccac cuccaggaga ugcgcgccau ccuggacaag     1560

caggccaagu ucuaccccuu ccucgccaag aacaaggagc ggaucgagaa gauccugacc     1620

uuccgcaucc ccuacuacgu gggcccccug gcgcggggga acagcgacuu cgccuggucc     1680

auccgcaagc ggaacgagaa gaucaccccg uggaacuucg aggacgugau cgacaaggag     1740

agcuccgccg aggccuucau caaccgcaug acgagcuucg accucuaccu gcccgaggag     1800

aagguccugc ccaagcacuc ccuccuguac gagaccuuca acguguacaa cgagcugacc     1860

aaggugcggu ucaucgccga gagcaugcgc gacuaccagu uccucgacuc caagcagaag     1920

aaggacaucg uccggcugua cuucaaggac aagcgcaagg ugaccgacaa ggacaucauc     1980

gaguaccugc acgcgaucua cggcuacgac ggcaucgagc ucaaggggau cgagaagcag     2040

uucaacagcu cccugagcac guaccacgac cugcucaaca ucaucaacga caaggaguuc     2100

cuggacgacu ccagcaacga ggccaucauc gaggagauca uccacacccu gaccaucuuc     2160

gaggaccggg agaugaucaa gcagcgccuc uccaaguucg agaacaucuu cgacaagagc     2220

gugcugaaga agcugagccg gcgccacuac accggcuggg ggaagcucuc cgccaagcug     2280

aucaacggca uccgggacga gaaguccggg aacacgaucc uggacuaccu caucgacgac     2340

ggcaucagca accgcaacuu caugcagcug auccacgacg acgcccugag cuucaagaag     2400

aagauccaga aggcgcagau caucggcgac gaggacaagg ggaacaucaa ggaggucgug     2460

aagucccucc ccggcagccc ggccaucaag aaggggaucc ugcaguccau caagaucguc     2520

gacgagcugg ugaaggugau gggcgggcgg aagcccgaga gcaucgucgu ggagauggcc     2580

cgcgagaacc aguacaccaa ccagggcaag uccaacagcc agcagcggcu caagcgccug     2640

gagaaguccc ugaaggagcu cggcagcaag auccugaagg agaacauccc cgccaagcug     2700

uccaagaucg acaacaacgc ccuccagaac gaccgccugu accuguacua ccuccagaac     2760

gggaaggaca uguacaccgg cgacgaccug gacaucgacc ggcugagcaa cuacgacauc     2820

gaccacauca ucccccaggc guuccucaag gacaacucca ucgacaacaa ggugcugguc     2880

agcuccgcca gcaaccgcgg gaaguccgac gacuucccga gccuggaggu ggugaagaag     2940

cggaagaccu ucugguacca gcuccucaag agcaagcuga ucucccagcg caaguucgac     3000

aaccugacga aggccgagcg gggcggccug cugcccgagg acaaggccgg guucauccag     3060

cgccagcucg ucgagacccg gcagaucacc aagcacgugg cccgccugcu ggacgagaag     3120

uucaacaaca agaaggacga gaacaaccgg gcggugcgca ccgucaagau caucacgcuc     3180

aagagcaccc ugguguccca guuccggaag gacuucgagc uguacaaggu gcgcgagauc     3240

aacgacuucc accacgccca cgacgccuac cucaacgccg ucaucgccag cgcgcugcug     3300

aagaaguacc ccaagcucga gcccgaguuc guguacggcg acuacccgaa guacaacucc     3360

uuccgggagc gcaagagcgc caccgagaag guguacuucu acuccaacau caugaacauc     3420

uucaagaagu ccaucagccu ggccgacggg cgggucaucg agcgcccccu gaucgaggug     3480

aacgaggaga ccggcgagag cguguggaac aaggaguccg accucgccac cguccggcgc     3540

gugcugagcu acccccaggu gaacgucgug aagaaggugg aggagcagaa ccacgggcug     3600

gaccggggca agcccaaggg ccucuucaac gcgaaccugu ccagcaagcc gaagcccaac     3660

uccaacgaga accuggucgg ggccaaggag uaccucgacc ccaagaagua cggcggguac     3720

gccggcauca gcaacuccuu cgccgugcug gugaagggga cgaucgagaa gggcgccaag     3780

aagaagauca ccaacguccu ggaguuccag ggcaucagca uccucgaccg caucaacuac     3840

cggaaggaca agcugaacuu ccugcucgag aagggguaca aggacaucga gcugaucauc     3900

gagcugccga aguacagccu cuucgagcug uccgacggca gccgccgcau gcuggcgucc     3960

auccucagca ccaacaacaa gcgcggggag auccacaagg gcaaccagau cuuccugucc     4020

cagaaguucg ugaagcugcu cuaccacgcc aagcggauca gcaacaccau caacgagaac     4080

caccggaagu acguggagaa ccacaagaag gaguucgagg agcuguucua cuacauccug     4140

gaguucaacg agaacuacgu cggggccaag aagaacggca agcuccugaa cuccgccuuc     4200

cagagcuggc agaaccacuc caucgacgag cugugcagcu ccuucaucgg ccccacgggg     4260

agcgagcgca agggccucuu cgagcugacc ucccggggga gcgccgcgga cuucgaguuc     4320

cugggcguga agaucccccg cuaccgggac uacacccccu ccagccuccu gaaggacgcc     4380

acccugaucc accaguccgu gacggggcug uacgagaccc gcaucgaccu cgccaagcug     4440

ggcgagggca agcggccggc cgccaccaag aaggcggggc aggccaagaa gaagaaguaa     4500

ggacuaguaa uaaggaaacu guaugaaugu cugugggcag gaagugaaga gauccuucug     4560

uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu uuugaaauaa uaaaucuggu     4620

agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaag aauu                                            4704


<210>  2462
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2462
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcguggua cgccagccug      240

auguccgccc accaccuccg ggugggcauc gacgugggga cccacagcgu cggccuggcc      300

acccugcgcg uggacgacca cgggacgccc aucgagcucc uguccgcccu gagccacauc      360

cacgacuccg gcgugggcaa ggaggggaag aaggaccacg acacccggaa gaagcucagc      420

ggcaucgcgc gccgggcccg ccggcugcug caccaccgcc ggacccagcu ccagcagcug      480

gacgaggucc ugcgcgaccu cggguucccc auccccaccc cgggcgaguu ccuggaccug      540

aacgagcaga cggaccccua ccgggugugg cgcgugcggg cccgccucgu cgaggagaag      600

cugcccgagg agcugcgggg gcccgccauc uccauggcgg ugcgccacau cgcccggcac      660

cgcggcuggc ggaacccgua cagcaaggug gagucccucc ugagccccgc cgaggagucc      720

cccuucauga aggcccugcg cgagcggauc cucgccacca ccggcgaggu ccuggacgac      780

gggaucaccc ccggccaggc gauggcccag guggcccuga cgcacaacau cagcaugcgc      840

gggccggagg gcauccucgg caagcugcac caguccgaca acgccaacga gauccgcaag      900

aucugcgccc ggcagggggu gagccccgac gucugcaagc agcugcuccg cgcgguguuc      960

aaggccgaca gcccccgggg cuccgccgug ucccgcgucg cccccgaccc gcugcccggg     1020

cagggcagcu uccggcgcgc ccccaagugc gaccccgagu uccagcgguu ccgcaucauc     1080

uccaucgugg cgaaccugcg gaucagcgag accaaggggg agaaccgccc gcucaccgcc     1140

gacgagcggc gccacguggu caccuuccug acggaggaca gccaggccga ccugaccugg     1200

guggacgugg ccgagaagcu cggcguccac cggcgcgacc ugcggggcac cgccgugcac     1260

accgacgacg gggagcgcuc cgcggcccgg ccgcccaucg acgccacgga ccgcaucaug     1320

cggcagacca agaucagcuc ccugaagacc uggugggagg aggccgacag cgagcagcgc     1380

ggcgcgauga uccgcuaccu cuacgaggac cccaccgacu ccgagugcgc cgagaucauc     1440

gccgagcugc ccgaggagga ccaggccaag cuggacagcc uccaccuccc cgccgggcgg     1500

gcggccuacu cccgcgagag ccugacggcc cuguccgacc acaugcuggc caccaccgac     1560

gaccugcacg aggcccggaa gcgccucuuc ggcgucgacg acagcugggc gccgcccgcc     1620

gaggccauca acgcccccgu ggggaacccc uccguggacc ggacccugaa gaucgucggc     1680

cgcuaccuga gcgccgugga guccaugugg ggcacgcccg aggugaucca cgucgagcac     1740

gugcgggacg gguucaccag cgagcgcaug gcggacgagc gggacaaggc caaccgccgg     1800

cgcuacaacg acaaccagga ggccaugaag aagauccagc gggacuacgg caaggagggg     1860

uacaucuccc gcggcgacau cgugcggcuc gacgcccugg agcugcaggg gugcgcgugc     1920

cuguacugcg gcaccaccau cggcuaccac acgugccagc ucgaccacau cgucccgcag     1980

gccgggcccg gcagcaacaa ccgccggggg aaccuggugg ccgugugcga gcgcugcaac     2040

cgguccaaga gcaacacccc cuucgccguc ugggcccaga agugcggcau cccccacgug     2100

ggcgugaagg aggcgaucgg gcgcguccgg ggcuggcgca agcagacccc gaacaccucc     2160

agcgaggacc ugacgcgccu caagaaggag gugaucgccc ggcugcgccg gacccaggag     2220

gaccccgaga ucgacgagcg cagcauggag uccguggccu ggauggccaa cgagcugcac     2280

caccggaucg ccgcggccua ccccgagacc accgucaugg uguaccgcgg gagcaucacg     2340

gccgccgccc ggaaggcggc cggcaucgac ucccgcauca accucaucgg ggagaagggc     2400

cggaaggacc gcaucgaccg gcgccaccac gccguggacg ccagcgucgu ggcgcugaug     2460

gaggccuccg uggccaagac ccuggccgag cggagcuccc uccgcggcga gcagcggcug     2520

accgggaagg agcagaccug gaagcaguac accggcagca cggucggggc ccgcgagcac     2580

uucgagaugu ggcggggcca caugcugcac cucaccgagc uguucaacga gcgccuggcg     2640

gaggacaagg uguacgugac ccagaacauc cggcuccgcc uguccgacgg gaacgcccac     2700

accgucaacc ccagcaagcu ggugucccac cgccucggcg acggccugac ggugcagcag     2760

aucgaccggg ccugcacccc ggcccugugg ugcgcccuca cccgcgagaa ggacuucgac     2820

gagaagaacg ggcugcccgc gcgggaggac cgcgccaucc ggguccacgg ccacgagauc     2880

aagagcuccg acuacaucca gguguucagc aagcgcaaga agaccgacuc cgaccgggac     2940

gagacgcccu ucggggccau cgccgugcgc ggcggguucg ucgagaucgg ccccagcauc     3000

caccacgccc ggaucuaccg cguggagggc aagaagccgg uguacgcgau gcugcggguc     3060

uucacccacg accuccuguc ccagcgccac ggggaccugu ucagcgccgu gaucccgccc     3120

cagagcaucu ccaugcggug cgccgagccc aagcuccgca aggccaucac caccggcaac     3180

gccacguacc uggggugggu ggucgugggc gacgagcugg agaucaacgu ggacagcuuc     3240

accaaguacg cgaucgggcg guuccucgag gacuucccca acaccacccg cuggcggauc     3300

ugcggcuacg acacgaacuc caagcugacc cugaagccca ucguccucgc cgccgagggc     3360

cuggagaacc ccagcuccgc cgugaacgag aucguggagc ugaaggggug gcgcgucgcg     3420

aucaacgugc ucaccaaggu gcaccccacc gucgugcggc gcgacgcccu gggccggccg     3480

cgcuacagcu cccgcagcaa ccugcccacg uccuggacca ucgagaagcg gcccgccgcc     3540

accaagaagg ccgggcaggc gaagaagaag aaguaaggac uaguaauaag gaaacuguau     3600

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     3660

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3780


<210>  2463
<211>  4641
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2463
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaccaa gcccuacagc      240

aucggccugg acaucgggac caacuccgug ggcugggccg ugacgaccga caacuacaag      300

guccccagca agaagaugaa ggugcucggg aacaccucca agaaguacau caagaagaac      360

cugcugggcg ugcuccuguu cgacagcggc aucaccgccg aggggcggcg ccugaagcgg      420

acggcccgcc ggcgcuacac ccggcgccgg aaccgcaucc ucuaccugca ggagaucuuc      480

uccaccgaga uggccacccu ggacgacgcg uucuuccagc ggcucgacga cagcuuccug      540

guccccgacg acaagcgcga cuccaaguac ccgaucuucg gcaaccuggu ggaggagaag      600

gccuaccacg acgaguuccc cacgaucuac caccuccgga aguaccuggc cgacagcacc      660

aagaaggccg accugcgccu cguguaccug gcgcuggccc acaugaucaa guaccggggg      720

cacuuccuca ucgagggcga guucaacucc aagaacaacg acauccagaa gaacuuccag      780

gacuuccugg acaccuacaa cgccaucuuc gagagcgacc ugucccucga gaacagcaag      840

cagcuggagg agaucgucaa ggacaagauc uccaagcugg agaagaagga ccgcauccuc      900

aagcuguucc ccggggagaa gaacagcggc aucuucuccg aguuccugaa gcucaucgug      960

ggcaaccagg ccgacuuccg gaagugcuuc aaccuggacg agaaggccag ccugcacuuc     1020

uccaaggaga gcuacgacga ggaccucgag acccugcugg gguacaucgg cgacgacuac     1080

uccgacgugu uccucaaggc gaagaagcug uacgacgcca uccugcucag cggguuccug     1140

acggucaccg acaacgagac cgaggcgccc cugagcuccg ccaugaucaa gcgcuacaac     1200

gagcacaagg aggaccucgc ccugcugaag gaguacaucc ggaacaucag ccucaagacc     1260

uacaacgagg uguucaagga cgacacgaag aacggcuacg ccggcuacau cgacgggaag     1320

accaaccagg aggacuucua cguguaccug aagaagcugc ucgccgaguu cgagggcgcc     1380

gacuacuucc uggagaagau cgaccgcgag gacuuccugc ggaagcagcg caccuucgac     1440

aacgggucca ucccguacca gauccaccuc caggagaugc gcgccauccu ggacaagcag     1500

gccaaguucu accccuuccu ggcgaagaac aaggagcgga ucgagaagau ccugaccuuc     1560

cgcauccccu acuacgucgg cccccucgcc cgggggaaca gcgacuucgc cugguccauc     1620

cgcaagcgga acgagaagau cacgccgugg aacuucgagg acgugaucga caaggagagc     1680

uccgccgagg ccuucaucaa ccgcaugacc agcuucgacc uguaccugcc cgaggagaag     1740

gugcucccca agcacucccu gcuguacgag accuucaacg ucuacaacga gcucaccaag     1800

gugcgguuca ucgcggagag caugcgcgac uaccaguucc uggacuccaa gcagaagaag     1860

gacaucgugc ggcuguacuu caaggacaag cgcaagguca cggacaagga caucaucgag     1920

uaccuccacg ccaucuacgg cuacgacggc aucgagcuga aggggaucga gaagcaguuc     1980

aacagcuccc ugagcaccua ccacgaccuc cugaacauca ucaacgacaa ggaguuccug     2040

gacgacucca gcaacgaggc caucaucgag gagaucaucc acacccucac caucuucgag     2100

gaccgggaga ugaucaagca gcgccugucc aaguucgaga acaucuucga caagagcgug     2160

cugaagaagc ucucccggcg ccacuacacg ggcuggggga agcugagcgc caagcugauc     2220

aacggcaucc gggacgagaa guccgggaac accauccucg acuaccugau cgacgacggc     2280

aucagcaacc gcaacuucau gcagcugauc cacgacgacg cgcucagcuu caagaagaag     2340

auccagaagg cccagaucau cggcgacgag gacaagggga acaucaagga gguggucaag     2400

ucccugcccg gcagcccggc caucaagaag gggauccugc aguccaucaa gaucguggac     2460

gagcucguca aggugauggg cgggcggaag cccgagagca ucguggucga gauggcccgc     2520

gagaaccagu acaccaacca gggcaagucc aacagccagc agcggcugaa gcgccuggag     2580

aagucccuca aggagcuggg cagcaagauc cugaaggaga acauccccgc caagcucucc     2640

aagaucgaca acaacgcgcu gcagaacgac cgccuguacc ucuacuaccu gcagaacggg     2700

aaggacaugu acaccggcga cgaccuggac aucgaccggc ucagcaacua cgacaucgac     2760

cacaucaucc cccaggccuu ccugaaggac aacuccaucg acaacaaggu gcuggugagc     2820

uccgccagca accgcgggaa guccgacgac gucccgagcc ucgagguggu gaagaagcgg     2880

aagacguucu gguaccagcu gcucaagagc aagcugaucu cccagcgcaa guucgacaac     2940

cugaccaagg ccgagcgggg cggccugagc cccgaggaca aggccggguu cauccagcgc     3000

cagcucgucg agacccggca gaucaccaag cacguggcgc gccugcugga cgagaaguuc     3060

aacaacaaga aggacgagaa caaccgggcc gugcgcacgg ucaagaucau cacccucaag     3120

uccacccugg ugagccaguu ccggaaggac uucgagcugu acaaggugcg cgagaucaac     3180

gacuuccacc acgcccacga cgccuaccuc aacgccgucg uggcguccgc ccugcugaag     3240

aaguacccca agcucgagcc cgaguucgug uacggcgacu acccgaagua caacagcuuc     3300

cgggagcgca aguccgccac cgagaagguc uacuucuaca gcaacaucau gaacaucuuc     3360

aagaagucca ucagccuggc cgacgggcgg gugaucgagc gcccccugau cgaggugaac     3420

gaggagaccg gcgaguccgu cuggaacaag gagagcgacc ucgcgacggu gcggcgcgug     3480

cuguccuacc cccaggucaa cguggugaag aaggucgagg agcagaacca cgggcuggac     3540

cggggcaagc ccaagggccu cuucaacgcc aaccugagcu ccaagccgaa gcccaacagc     3600

aacgagaacc uggugggggc caaggaguac cucgacccca agaaguacgg cggguacgcc     3660

ggcaucucca acagcuucac cgugcugguc aaggggacca ucgagaaggg cgccaagaag     3720

aagaucacca acgugcugga guuccagggc aucuccaucc ucgaccgcau caacuaccgg     3780

aaggacaagc ugaacuuccu gcucgagaag ggguacaagg acaucgagcu gaucaucgag     3840

cugccgaagu acagccucuu cgagcugagc gacggcuccc gccgcaugcu ggcgagcauc     3900

cucuccacga acaacaagcg cggggagauc cacaagggca accagaucuu ccugagccag     3960

aaguucguga agcugcucua ccacgccaag cggaucucca acaccaucaa cgagaaccac     4020

cggaaguacg ucgagaacca caagaaggag uucgaggagc uguucuacua cauccuggag     4080

uucaacgaga acuacguggg ggccaagaag aacggcaagc uccugaacag cgccuuccag     4140

uccuggcaga accacagcau cgacgagcug ugcuccagcu ucaucggccc caccgggucc     4200

gagcgcaagg gccucuucga gcugaccagc cggggguccg ccgcggacuu cgaguuccug     4260

ggcgugaaga ucccccgcua ccgggacuac acgcccagcu cccuccugaa ggacgccacc     4320

cugauccacc agagcgucac cgggcucuac gagacccgca ucgaccuggc caagcugggc     4380

gagggcaagc ggccggccgc cacgaagaag gcggggcagg ccaagaagaa gaaguaagga     4440

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     4500

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     4560

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaagaau u                                               4641


<210>  2464
<211>  3723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2464
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggccgc cuucaagccc      240

aacagcauca acuacauccu gggccucgac aucgggaucg ccuccguggg cugggccaug      300

guggagaucg acgaggagga gaaccccauc cggcugaucg accugggggu ccgcguguuc      360

gagcgggcgg aggugcccaa gaccggcgac agccucgcca uggcccgccg gcuggcccgc      420

uccguccggc gccugacccg gcgccgggcg caccgccucc ugcggacgcg ccggcugcuc      480

aagcgcgagg gcgugcugca ggccgccaac uucgacgaga acgggcugau caagagccuc      540

ccgaacaccc ccuggcagcu gcgggccgcc gcgcuggacc gcaagcucac gccccuggag      600

ugguccgccg ugcugcucca ccugaucaag caccggggcu accugagcca gcgcaagaac      660

gagggggaga ccgccgacaa ggagcucggc gcccugcuga agggggucgc cggcaacgcg      720

cacgcccucc agaccggcga cuuccggacc cccgccgagc uggcccugaa caaguucgag      780

aaggaguccg ggcacauccg caaccagcgc agcgacuacu cccacaccuu cagccggaag      840

gaccuccagg ccgagcugau ccuccuguuc gagaagcaga aggaguucgg caacccgcac      900

guguccgggg gccugaagga gggcaucgag acccugcuca ugacgcagcg ccccgcgcug      960

agcggggacg ccgugcagaa gaugcugggc cacugcaccu ucgagcccgc cgagcccaag     1020

gccgccaaga acaccuacac cgcggagcgg uucaucuggc ucacgaagcu gaacaaccug     1080

cgcauccucg agcagggguc cgagcggccg cugaccgaca ccgagcgcgc cacccugaug     1140

gacgagcccu accgcaagag caagcucacg uacgcccagg cccggaagcu gcugggccuc     1200

gaggacaccg cguucuucaa ggggcugcgg uacggcaagg acaacgccga ggccuccacc     1260

cugauggaga ugaaggccua ccacgccauc agccgcgcgc ucgagaagga gggccugaag     1320

gacaagaagu ccccgcugaa ccugagcccc gagcuccagg acgagaucgg gaccgccuuc     1380

ucccuguuca agacggacga ggacaucacc ggccggcuga aggaccgcau ccagccggag     1440

auccucgagg cccugcugaa gcacaucagc uucgacaagu ucguccagau cagccucaag     1500

gcccugcggc gcaucgugcc ccugauggag caggggaagc gguacgacga ggccugcgcg     1560

gagaucuacg gcgaccacua cgggaagaag aacaccgagg agaagaucua ccucccgccc     1620

auccccgccg acgagauccg caaccccgug guccugcggg cccuguccca ggcccgcaag     1680

gugaucaacg gcgugguccg gcgcuacggc agccccgccc gcauccacau cgagaccgcg     1740

cgggaggugg ggaaguccuu caaggaccgc aaggagaucg agaagcggca ggaggagaac     1800

cgcaaggacc gggagaaggc cgccgccaag uuccgcgagu acuuccccaa cuucgugggc     1860

gagcccaaga gcaaggacau ccucaagcug cggcuguacg agcagcagca cgggaagugc     1920

cucuacuccg gcaaggagau caaccugggg cgccugaacg agaagggcua cgucgagauc     1980

gaccacgcgc uccccuucag ccggacgugg gacgacuccu ucaacaacaa ggugcuggug     2040

cugggcagcg agaaccagaa caaggggaac cagacccccu acgaguacuu caacggcaag     2100

gacaacuccc gcgaguggca ggaguucaag gcccgggucg agaccagccg cuucccccgg     2160

uccaagaagc agcgcauccu ccugcagaag uucgacgagg acggguucaa ggagcggaac     2220

cugaacgaca cccgcuacgu gaaccgguuc cucugccagu ucgucgccga ccgcaugcgg     2280

cugacgggca agggcaagaa gcgcguguuc gccagcaacg ggcagaucac caaccugcuc     2340

cggggcuucu gggggcugcg caaggugcgc gccgagaacg accggcacca cgcgcuggac     2400

gccgucgugg uggccugcuc caccgucgcc augcagcaga agaucacccg cuucgugcgg     2460

uacaaggaga ugaacgccuu cgacggcaag acgaucgaca aggagaccgg ggaggugcuc     2520

caccagaaga cccacuuccc gcagcccugg gaguucuucg cgcaggaggu caugauccgc     2580

guguucggca agcccgacgg caagccggag uucgaggagg ccgacacccu ggagaagcug     2640

cggacgcucc uggccgagaa gcugagcucc cgccccgagg ccgugcacga guacgucacg     2700

ccccucuucg ugagccgggc ccccaaccgc aagauguccg ggcagggcca cauggagacc     2760

gucaagagcg cgaagcggcu ggacgagggg guguccgugc ugcgcgugcc gcucacccag     2820

cugaagcuga aggaccucga gaagaugguc aaccgggagc gcgagcccaa gcuguacgag     2880

gcccugaagg cccggcucga ggcccacaag gacgaccccg cgaaggccuu cgccgagccc     2940

uucuacaagu acgacaaggc cggcaaccgc acccagcagg ucaaggccgu gcggguggag     3000

cagguccaga agaccggggu gugggugcgc aaccacaacg gcaucgcgga caacgccacc     3060

auggugcggg uggacgucuu cgagaagggc gacaaguacu accuggugcc gaucuacagc     3120

uggcaggucg ccaaggggau ccugcccgac cgcgccgugg ugcagggcaa ggacgaggag     3180

gacuggcagc ucaucgacga cagcuucaac uucaaguucu cccugcaccc caacgaccug     3240

guggagguca ucaccaagaa ggcccggaug uucggguacu ucgcgagcug ccaccgcggc     3300

accgggaaca ucaacauccg cauccacgac cucgaccaca agaucggcaa gaacggcauc     3360

cuggagggga ucggcgugaa gacggcccug uccuuccaga aguaccagau cgacgagcuc     3420

gggaaggaga uccggcccug ccgccugaag aagcggccgc ccgugcgcaa gcggcccgcc     3480

gccaccaaga aggccggcca ggcgaagaag aagaaguaag gacuaguaau aaggaaacug     3540

uaugaauguc ugugggcagg aagugaagag auccuucugu aaagguuuuu ggaauuaugu     3600

cugcugaaua auaaacuuuu uugaaauaau aaaucuggua gaaaaaugag aucuaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     3720

auu                                                                   3723


<210>  2465
<211>  4479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2465
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagaa gcccuacacc      240

aucggccugg acaucgggac caacagcgug ggcugggccg ugcucacgga ccaguacgac      300

cuggucaagc ggaagaugaa gaucgccggg gacuccgaga agaagcagau caagaagaac      360

uucuggggcg ugcgccuguu cgacgagggc cagaccgccg ccgaccggcg cauggcgcgg      420

accgcccgcc ggcgcaucga gcggcgccgg aaccgcauca gcuaccucca ggggaucuuc      480

gccgaggaga uguccaagac cgacgccaac uucuucugcc ggcugagcga cuccuucuac      540

guggacaacg agaagcgcaa cagccggcac cccuucuucg cgacgaucga ggaggagguc      600

gaguaccaca agaacuaccc caccaucuac caccuccgcg aggagcuggu gaacuccagc      660

gagaaggccg accugcggcu gguguaccuc gcccuggccc acaucaucaa guaccgcggc      720

aacuuccuga ucgagggggc ccucgacacc cagaacaccu ccgucgacgg caucuacaag      780

caguucaucc agacguacaa ccagguguuc gcgagcggga ucgaggacgg cucccugaag      840

aagcuggagg acaacaagga cguggccaag auccucgucg agaaggugac ccggaaggag      900

aagcuggagc gcauccugaa gcucuacccg ggcgagaagu ccgccgggau guucgcccag      960

uucaucagcc ugaucguggg cagcaagggg aacuuccaga agcccuucga ccugaucgag     1020

aaguccgaca ucgagugcgc caaggacagc uacgaggagg accucgaguc ccugcuggcg     1080

cucaucggcg acgaguacgc cgagcuguuc gucgccgcca agaacgccua cagcgcggug     1140

gugcugucca gcaucaucac cgucgccgag accgagacga acgccaagcu cagcgccucc     1200

augaucgagc gguucgacac ccacgaggag gaccugggcg agcugaaggc cuucaucaag     1260

cuccaccucc ccaagcacua cgaggagauc uucagcaaca ccgagaagca cggguacgcg     1320

ggcuacaucg acgggaagac caagcaggcc gacuucuaca aguacaugaa gaugacgcug     1380

gagaacaucg agggcgccga cuacuucauc gccaagaucg agaaggagaa cuuccugcgc     1440

aagcagcgca ccuucgacaa cggggcgauc ccccaccagc ugcaccugga ggagcucgag     1500

gccauccugc accagcaggc caaguacuac ccguuccuga aggagaacua cgacaagauc     1560

aagucccucg ugaccuuccg gauccccuac uucgugggcc cccuggccaa cggccagagc     1620

gaguucgccu ggcugacccg caaggcggac ggggagaucc ggcccuggaa caucgaggag     1680

aaggucgacu ucggcaaguc cgccguggac uucaucgaga agaugacgaa caaggacacc     1740

uaccucccga aggagaacgu gcugcccaag cacagccugu gcuaccagaa guaccucguc     1800

uacaacgagc ugaccaaggu gcgcuacauc aacgaccagg ggaagaccuc cuacuucagc     1860

ggccaggaga aggagcagau cuucaacgac cuguucaagc agaagcggaa ggucaagaag     1920

aaggaccucg agcuguuccu gcgcaacaug ucccacgugg agagccccac gaucgagggg     1980

cuggaggacu ccuucaacag cuccuacagc accuaccacg accuccugaa ggugggcauc     2040

aagcaggaga uccuggacaa ccccgucaac accgagaugc ucgagaacau cgugaagauc     2100

cugaccgugu ucgaggacaa gcggaugauc aaggagcagc ugcagcaguu cuccgacguc     2160

cucgacggcg uggugcugaa gaagcuggag cgccggcacu acacggggug gggccgccuc     2220

agcgccaagc ugcugauggg gauccgggac aagcaguccc accucaccau ccuggacuac     2280

cugaugaacg acgacggccu caaccgcaac cugaugcagc ugaucaacga cagcaaccuc     2340

uccuucaaga gcaucaucga gaaggagcag gucaccaccg ccgacaagga cauccagagc     2400

aucguggccg accuggcggg guccccggcc aucaagaagg gcauccugca gagccucaag     2460

aucguggacg agcuggucuc cgugaugggc uacccgcccc agacgaucgu ggucgagaug     2520

gcccgggaga accagaccac cgggaagggc aagaacaaca gccgcccccg guacaagucc     2580

cuggagaagg ccaucaagga guucgggagc cagauccuca aggagcaccc caccgacaac     2640

caggagcugc gcaacaaccg gcuguaccuc uacuaccugc agaacggcaa ggacauguac     2700

acgggccagg accuggacau ccacaaccuc uccaacuacg acaucgacca caucgugccc     2760

cagagcuuca ucaccgacaa cuccaucgac aaccuggugc ugaccagcuc cgccgggaac     2820

cgcgagaagg gcgacgacgu cccgccccuc gagaucgugc ggaagcgcaa gguguucugg     2880

gagaagcugu accaggggaa ccugaugagc aagcgcaagu ucgacuaccu caccaaggcg     2940

gagcggggcg ggcugaccga ggccgacaag gcccgcuuca uccaccggca gcuggucgag     3000

acgcgccaga ucaccaagaa cguggccaac auccuccacc agcgguucaa cuacgagaag     3060

gacgaccacg gcaacaccau gaagcagguc cgcaucguga cccugaaguc cgcgcuggug     3120

agccaguucc ggaagcaguu ccagcucuac aaggugcgcg acgucaacga cuaccaccac     3180

gcccacgacg ccuaccugaa cggcguggug gccaacacgc ugcucaaggu cuacccccag     3240

cuggagcccg aguucgugua cggggacuac caccaguucg acugguucaa ggccaacaag     3300

gcgaccgcca agaagcaguu cuacaccaac aucaugcugu ucuucgccca gaaggaccgg     3360

aucaucgacg agaacggcga gauccucugg gacaagaagu accuggacac cgugaagaag     3420

gucauguccu accgccagau gaacaucgug aagaagacgg agauccagaa gggggaguuc     3480

agcaaggcca ccaucaagcc caagggcaac uccagcaagc ugaucccccg gaagaccaac     3540

ugggacccga ugaaguacgg gggccucgac agccccaaca uggccuacgc gguggucauc     3600

gaguacgcca agggcaagaa caagcuggug uucgagaaga agaucauccg cgugaccauc     3660

auggagcgga aggccuucga gaaggacgag aaggccuucc uggaggagca gggguaccgc     3720

cagcccaagg uccucgccaa gcugccgaag uacacgcugu acgagugcga ggagggccgg     3780

cgccggaugc ucgcguccgc caacgaggcc cagaagggga accagcaggu ccugcccaac     3840

caccugguga cccuccugca ccacgccgcg aacugcgagg ugagcgacgg caagucccug     3900

gacuacaucg agagcaaccg cgagauguuc gccgagcucc uggcccacgu guccgaguuc     3960

gccaagcggu acacccuggc cgaggcgaac cucaacaaga ucaaccagcu guucgagcag     4020

aacaaggagg gggacaucaa ggccaucgcc cagagcuucg uggaccugau ggccuucaac     4080

gccaugggcg cgcccgccuc cuucaaguuc uucgagacca cgaucgagcg caagcgguac     4140

aacaaccuca aggagcugcu gaacagcacc aucaucuacc aguccaucac cggccucuac     4200

gagagccgca agcgccugga cgacaagcgg cccgccgcca ccaagaaggc cgggcaggcg     4260

aagaagaaga aguaaggacu aguaauaagg aaacuguaug aaugucugug ggcaggaagu     4320

gaagagaucc uucuguaaag guuuuuggaa uuaugucugc ugaauaauaa acuuuuuuga     4380

aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            4479


<210>  2466
<211>  4512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2466
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagaa gcccuacagc      240

aucggccugg acaucgggac caacuccgug ggcugggccg uggucaccga cgacuacaag      300

gugcccgcca agaagaugaa ggugcucggg aacacggaca agagccacau cgagaagaac      360

cugcugggcg cccuccuguu cgacuccggc aacaccgccg aggaccggcg ccugaagcgg      420

accgcgcgcc ggcgcuacac ccggcgccgg aaccgcaucc ucuaccugca ggagaucuuc      480

agcgaggaga uggggaaggu cgacgacucc uucuuccacc ggcuggagga cagcuuccuc      540

gugacggagg acaagcgcgg cgagcggcac cccaucuucg ggaaccugga ggaggaggug      600

aaguaccacg agaacuuccc gaccaucuac caccuccgcc aguaccuggc cgacaacccc      660

gagaaggucg accugcggcu gguguaccuc gcccuggccc acaucaucaa guuccgcggc      720

cacuuccuga ucgaggggaa guucgacacc cggaacaacg acgugcagcg ccucuuccag      780

gaguuccugg cggucuacga caacaccuuc gagaacucca gccugcagga gcagaacguc      840

cagguggagg agauccucac ggacaagauc uccaagagcg ccaagaagga ccgggugcug      900

aagcuguucc ccaacgagaa guccaacggc cgcuucgccg aguuccucaa gcugaucgug      960

ggcaaccagg ccgacuucaa gaagcacuuc gagcuggagg agaaggcccc gcuccaguuc     1020

agcaaggaca ccuacgagga ggagcuggag gugcugcucg cgcagaucgg ggacaacuac     1080

gccgagcugu uccuguccgc caagaagcuc uacgacagca uccugcuguc cggcauccuc     1140

accgucaccg acguggggac gaaggccccg cugagcgccu ccaugaucca gcgguacaac     1200

gagcaccaga uggaccuggc gcagcucaag caguucaucc gccagaagcu gagcgacaag     1260

uacaacgagg uguucuccga cgucagcaag gacggcuacg ccggcuacau cgacgggaag     1320

accaaccagg aggccuucua caaguaccug aagggccucc ugaacaagau cgaggggagc     1380

ggcuacuucc uggacaagau cgagcgcgag gacuuccucc ggaagcagcg caccuucgac     1440

aacgggucca ucccccacca gauccaccuc caggagaugc gggccaucau ccgccggcag     1500

gccgaguucu accccuuccu ggcggacaac caggaccgca ucgagaagcu gcugaccuuc     1560

cggauccccu acuacguggg cccgcuggcc cgcggcaaga gcgacuucgc cuggcugucc     1620

cgcaagagcg ccgacaagau cacgcccugg aacuucgacg agaucgugga caaggagucc     1680

agcgccgagg cguucaucaa ccggaugacc aacuacgacc ucuaccugcc caaccagaag     1740

guccugccca agcacucccu ccuguacgag aaguucaccg uguacaacga gcugaccaag     1800

gucaaguaca agacggagca ggggaagacc gccuucuucg acgccaacau gaagcaggag     1860

aucuucgacg gcguguucaa gguguaccgg aaggucacca aggacaagcu cauggacuuc     1920

cuggagaagg aguucgacga guuccgcauc guggaccuga ccgggcucga caaggagaac     1980

aagguguuca acgccagcua cggcacguac cacgaccugu gcaagauccu ggacaaggac     2040

uuccucgaca acuccaagaa cgagaagauc cuggaggaca ucguccugac ccucacccug     2100

uucgaggacc gggagaugau ccgcaagcgg cuggagaacu acagcgaccu ccugaccaag     2160

gagcagguca agaagcugga gcgccggcac uacacggggu ggggccgccu cuccgcggag     2220

cugauccacg gcauccggaa caaggagagc cgcaagacca uccuggacua ccucaucgac     2280

gacgggaacu ccaaccgcaa cuucaugcag cugaucaacg acgacgcccu gagcuucaag     2340

gaggagaucg ccaaggccca ggugaucggc gagaccgaca accucaacca gguggugucc     2400

gacaucgccg ggagcccggc gaucaagaag ggcauccugc agucccugaa gaucguggac     2460

gagcucguca agaucauggg gcaccagccc gagaacaucg ugguggagau ggcccgggag     2520

aaccaguuca ccaaccaggg ccgccggaac agccagcagc gccugaaggg ccugacggac     2580

uccaucaagg aguucgggag ccagauccuc aaggagcacc ccgucgagaa cagccagcug     2640

cagaacgacc ggcuguuccu cuacuaccug cagaacggcc gcgacaugua caccggggag     2700

gagcuggaca ucgacuaccu cucccaguac gacaucgacc acaucauccc ccaggccuuc     2760

aucaaggaca acagcaucga caaccgggug cugaccucca gcaaggagaa ccgcggcaag     2820

uccgacgacg ugccgagcaa ggacgucgug cggaagauga aguccuacug gagcaagcug     2880

cucuccgcca agcugaucac ccagcgcaag uucgacaacc ugacgaaggc cgagcggggc     2940

gggcucaccg acgacgacaa ggcgggcuuc aucaagcgcc agcuggugga gacccggcag     3000

aucaccaagc acgucgcccg cauccuggac gagcgguuca acaccgagac ggacgagaac     3060

aacaagaaga uccgccaggu gaagaucgug acccucaaga gcaaccuggu cuccaacuuc     3120

cggaaggagu ucgagcugua caaggugcgc gagaucaacg acuaccacca cgcccacgac     3180

gccuaccuca acgccgugau cgggaaggcg cugcugggcg ucuaccccca gcucgagccc     3240

gaguucgugu acggggacua cccccacuuc cacggccaca aggagaacaa ggccaccgcc     3300

aagaaguucu ucuacagcaa caucaugaac uucuucaaga aggacgacgu gcggaccgac     3360

aagaacggcg agaucaucug gaagaaggac gagcacaucu ccaacaucaa gaagguccug     3420

agcuaccccc aggugaacau cgugaagaag gucgaggagc agaccggggg cuucuccaag     3480

gagagcaucc ugcccaaggg gaacuccgac aagcucaucc cccgcaagac gaagaaguuc     3540

uacugggaca ccaagaagua cggcggguuc gacagcccca ucguggccua cagcauccug     3600

gugaucgcgg acaucgagaa gggcaagucc aagaagcuga agaccgucaa ggcccucgug     3660

ggcgugacga ucauggagaa gaugaccuuc gagcgggacc cggucgccuu ccuggagcgc     3720

aagggguacc gcaacgugca ggaggagaac aucaucaagc ugcccaagua cagccucuuc     3780

aagcuggaga acggccggaa gcgccugcuc gccuccgccc gggagcugca gaaggggaac     3840

gagaucgugc ugcccaacca ccucggcacc cugcuguacc acgcgaagaa cauccacaag     3900

gucgacgagc cgaagcaccu cgacuacgug gacaagcaca aggacgaguu caaggagcug     3960

cuggacgugg ucagcaacuu cuccaagaag uacacccucg ccgaggggaa ccuggagaag     4020

aucaaggagc uguacgccca gaacaacggc gaggaccuca aggagcuggc cagcuccuuc     4080

aucaaccugc ucacguucac cgccaucggc gcgcccgcca ccuucaaguu cuucgacaag     4140

aacaucgacc gcaagcggua caccagcacg accgagaucc ugaacgccac ccugauccac     4200

caguccauca ccgggcucua cgagacgcgc aucgaccuga acaagcuggg cggggacaag     4260

cggcccgccg ccaccaagaa ggcgggccag gccaagaaga agaaguaagg acuaguaaua     4320

aggaaacugu augaaugucu gugggcagga agugaagaga uccuucugua aagguuuuug     4380

gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua aaucugguag aaaaaugaga     4440

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaagaa uu                                                         4512


<210>  2467
<211>  3645
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2467
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgcagac caccaaccug      240

agcuacaucc ucggccugga ccuggggauc gccuccgugg gcugggccgu ggucgagauc      300

aacgagaacg aggaccccau cgggcucauc gacgugggcg ugcggaucuu cgagcgcgcc      360

gaggucccca agacgggcga gagccuggcc cugucccggc gccucgcgcg gagcacccgc      420

cggcugaucc gccggcgcgc ccaccggcug cuccuggcca agcgcuuccu gaagcgggag      480

gggauccucu ccaccaucga ccuggagaag ggccugccca accaggccug ggagcuccgc      540

guggcggggc uggagcggcg ccugagcgcc aucgaguggg gcgccgugcu ccugcaccug      600

aucaagcacc ggggguaccu cuccaagcgc aagaacgaga gccagaccaa caacaaggag      660

cugggcgccc ugcucuccgg cgucgcccag aaccaccagc ugcugcagag cgacgacuac      720

cggacgccgg cggagcucgc ccugaagaag uucgccaagg aggaggggca cauccgcaac      780

cagcggggcg ccuacaccca caccuucaac cgccuggacc uccuggccga gcugaaccuc      840

cuguucgcgc agcagcacca guucgggaac ccccacugca aggagcacau ccagcaguac      900

augaccgagc ugcucaugug gcagaagccc gcccuguccg gcgaggccau ccugaagaug      960

cucggcaagu gcacgcacga gaagaacgag uucaaggccg ccaagcacac cuacagcgcg     1020

gagcgcuucg uguggcugac caagcugaac aaccuccgga uccuggagga cggggccgag     1080

cgcgcccuga acgaggagga gcggcagcuc cugaucaacc accccuacga gaaguccaag     1140

cugaccuacg cccaggugcg caagcuccug ggccugagcg agcaggccau cuucaagcac     1200

cuccgguacu ccaaggagaa cgcggagagc gccacguuca uggagcucaa ggccuggcac     1260

gccauccgca aggcgcugga gaaccagggg cugaaggaca ccuggcagga ccucgccaag     1320

aagccggacc ugcuggacga gaucggcacc gccuucuccc ucuacaagac cgacgaggac     1380

auccagcagu accugacgaa caaggucccc aacagcguga ucaacgcccu gcucgugucc     1440

cugaacuucg acaaguucau cgagcugagc cucaagagcc ugcggaagau ccugccccuc     1500

auggagcagg ggaagcgcua cgaccaggcc ugccgggaga ucuacggcca ccacuacggc     1560

gaggcgaacc agaagaccuc ccagcugcug cccgccaucc cggcccagga gauccgcaac     1620

cccgucgugc uccggacccu gagccaggcc cgcaagguga ucaacgccau cauccggcag     1680

uacggguccc ccgcgcgcgu ccacaucgag accggccggg agcugggcaa gagcuucaag     1740

gagcgccggg agauccagaa gcagcaggag gacaaccgca cgaagcggga guccgccgug     1800

cagaaguuca aggagcuguu cagcgacuuc uccagcgagc ccaaguccaa ggacauccug     1860

aaguuccgcc uguacgagca gcagcacggg aagugccucu acagcgggaa ggagaucaac     1920

auccaccgcc ugaacgagaa gggcuacgug gagaucgacc acgcccugcc guucucccgg     1980

accugggacg acagcuucaa caacaagguc cucgugcugg ccuccgagaa ccagaacaag     2040

ggcaaccaga cccccuacga guggcugcag gggaagauca acagcgagcg cuggaagaac     2100

uucguggccc ucguccuggg cucccagugc agcgcggcca agaagcagcg gcugcucacc     2160

caggugaucg acgacaacaa guucaucgac cgcaaccuga acgacacgcg guacaucgcc     2220

cgcuuccugu ccaacuacau ccaggagaac cuccugcugg ucgggaagaa caagaagaac     2280

guguucaccc ccaacggcca gaucaccgcc cuccugcgga gccgcugggg gcugaucaag     2340

gcgcgggaga acaacaaccg ccaccacgcc cucgacgcca ucguggucgc cugcgccacc     2400

cccuccaugc agcagaagau cacgcgguuc auccgcuuca aggaggugca cccguacaag     2460

aucgagaacc gguacgagau gguggaccag gagagcggcg agaucaucag cccccacuuc     2520

cccgagcccu gggcguacuu ccgccaggag gucaacaucc ggguguucga caaccacccg     2580

gacaccgugc ugaaggagau gcugcccgac cgcccccagg ccaaccacca guucguccag     2640

ccccucuucg ugucccgggc cccgacccgc aagaugagcg gccaggggca cauggagacc     2700

aucaaguccg ccaagcggcu ggccgagggc aucagcgugc ugcgcauccc ccucacgcag     2760

cugaagccca accugcucga gaacaugguc aacaaggagc gggagccggc gcuguacgcc     2820

gggcugaagg cccgccucgc cgaguucaac caggaccccg ccaaggcguu cgccaccccc     2880

uucuacaagc agggcggcca gcaggucaag gccauccgcg uggagcaggu ccagaagucc     2940

ggggugcugg ugcgggagaa caacggcgug gccgacaacg cgagcaucgu gcgcaccgac     3000

guguucauca agaacaacaa guucuuccug guccccaucu acaccuggca ggucgccaag     3060

gggauccucc cgaacaaggc caucguggcc cacaagaacg aggacgagug ggaggagaug     3120

gacgagggcg ccaaguucaa guucucccug uuccccaacg accuggugga gcucaagacg     3180

aagaaggagu acuucuucgg guacuacauc ggccuggacc gggcgaccgg caacaucagc     3240

cugaaggagc acgacgggga gaucuccaag ggcaaggacg ggguguaccg cgugggcguc     3300

aagcucgccc ugagcuucga gaaguaccag guggacgagc uggggaagaa ccggcagauc     3360

ugccgccccc agcagcggca gcccgugcgc aagcggccgg ccgccaccaa gaaggccggc     3420

caggcgaaga agaagaagua aggacuagua auaaggaaac uguaugaaug ucugugggca     3480

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     3540

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3645


<210>  2468
<211>  3723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2468
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggccgc cuucaagccc      240

aaccccauca acuacauccu gggccucgac aucgggaucg ccagcguggg cugggccaug      300

guggagaucg acgaggacga gaaccccauc ugccugaucg accugggggu ccggguguuc      360

gagcgcgcgg aggugccgaa gaccggcgac ucccucgcca uggcccggcg ccuggcccgg      420

agcguccgcc ggcugacccg ccggcgcgcg caccggcucc ugcgcgcccg gcgccugcuc      480

aagcgggagg gcgugcugca ggccgccgac uucgacgaga acgggcugau caagucccuc      540

cccaacacgc ccuggcagcu gcgcgccgcg gcccuggacc ggaagcucac gccccuggag      600

uggagcgccg ugcugcucca ccugaucaag caccgcggcu accuguccca gcggaagaac      660

gagggggaga ccgccgacaa ggagcucggc gcccugcuga agggggucgc ggacaacgcc      720

cacgcccucc agaccggcga cuuccgcacc ccggccgagc uggcccugaa caaguucgag      780

aaggagagcg gccacauccg gaaccagcgc ggggacuacu cccacaccuu cagccgcaag      840

gaccuccagg cggagcugau ccuccucuuc gagaagcaga aggaguucgg caacccccac      900

guguccgggg gccugaagga gggcaucgag acccugcuca ugacccagcg gcccgcccug      960

agcggggacg ccgugcagaa gaugcugggc cacugcacgu ucgagcccgc cgagccgaag     1020

gccgcgaaga acaccuacac cgccgagcgc uucaucuggc ucaccaagcu gaacaaccug     1080

cggauccucg agcagggguc cgagcgcccc cugacggaca ccgagcgggc cacccugaug     1140

gacgagcccu accgcaagag caagcucacc uacgcccagg cgcggaagcu gcugggccuc     1200

gaggacacgg ccuucuucaa ggggcugcgc uacggcaagg acaacgccga ggccuccacc     1260

cugauggaga ugaaggccua ccacgcgauc agccgggccc ucgagaagga gggccugaag     1320

gacaagaagu ccccgcugaa ccugagcccc gagcuccagg acgagaucgg gaccgccuuc     1380

ucccuguuca agaccgacga ggacaucacg ggccgccuga aggaccggau ccagcccgag     1440

auccucgagg cccugcugaa gcacaucagc uucgacaagu ucguccagau cucccucaag     1500

gcccugcgcc ggaucgugcc ccugauggag caggggaagc gcuacgacga ggcgugcgcc     1560

gagaucuacg gcgaccacua cgggaagaag aacaccgagg agaagaucua ccugcccccg     1620

auccccgccg acgagauccg gaaccccgug guccugcgcg cccugagcca ggcccggaag     1680

gugaucaacg gcgugguccg ccgguacggc agccccgcgc gcauccacau cgagaccgcc     1740

cgcgaggugg ggaaguccuu caaggaccgg aaggagaucg agaagcgcca ggaggagaac     1800

cggaaggacc gcgagaaggc cgccgcgaag uuccgggagu acuucccgaa cuucgugggc     1860

gagcccaaga gcaaggacau ccucaagcug cgccuguacg agcagcagca cgggaagugc     1920

cucuacuccg gcaaggagau caaccugggg cggcugaacg agaagggcua cgucgagauc     1980

gaccacgccc uccccuucag ccgcaccugg gacgacuccu ucaacaacaa ggugcuggug     2040

cugggcagcg agaaccagaa caaggggaac cagacgcccu acgaguacuu caacggcaag     2100

gacaacuccc gggaguggca ggaguucaag gcccgcgucg agaccagccg guucccgcgc     2160

uccaagaagc agcggauccu ccugcagaag uucgacgagg acggguucaa ggagcgcaac     2220

cugaacgaca cccgguacgu gaaccgcuuc cucugccagu ucgucgccga ccggaugcgc     2280

cugaccggca agggcaagaa gcggguguuc gccagcaacg ggcagaucac gaaccugcuc     2340

cgcggcuucu gggggcugcg gaaggugcgc gcggagaacg accgccacca cgcccuggac     2400

gccgucgugg uggccugcuc caccgucgcc augcagcaga agaucacccg guucgugcgc     2460

uacaaggaga ugaacgcguu cgacggcaag accaucgaca aggagacggg ggaggugcuc     2520

caccagaaga cccacuuccc ccagcccugg gaguucuucg cccaggaggu caugauccgg     2580

guguucggca agccggacgg caagcccgag uucgaggagg ccgacacccc cgagaagcug     2640

cgcacccugc ucgccgagaa gcugagcucc cggcccgagg ccgugcacga guacgucacg     2700

ccgcuguucg ugagccgcgc gcccaaccgg aagauguccg ggcagggcca cauggagacc     2760

gucaagagcg ccaagcgccu cgacgagggg guguccgugc ugcgggugcc ccugacccag     2820

cucaagcuga aggaccugga gaagaugguc aaccgcgagc gggagcccaa gcucuacgag     2880

gcccugaagg cccgccugga ggcgcacaag gacgacccgg ccaaggccuu cgccgagccc     2940

uucuacaagu acgacaaggc cggcaaccgg acccagcagg ucaaggcggu gcgcguggag     3000

cagguccaga agaccggggu gugggugcgg aaccacaacg gcaucgccga caacgccacg     3060

auggugcgcg uggacgucuu cgagaagggc gacaaguacu accucgugcc caucuacagc     3120

uggcaggucg ccaaggggau ccugcccgac cgggccgugg ugcagggcaa ggacgaggag     3180

gacuggcagc ugaucgacga cuccuucaac uucaaguuca gccuccaccc gaacgaccug     3240

guggagguca ucaccaagaa ggcgcgcaug uucggguacu ucgccagcug ccaccggggc     3300

accgggaaca ucaacauccg cauccacgac cuggaccaca agaucggcaa gaacggcauc     3360

cucgagggga ucggcgugaa gaccgcccug uccuuccaga aguaccagau cgacgagcug     3420

gggaaggaga uccgccccug ccggcucaag aagcgcccgc ccgugcggaa gcgccccgcc     3480

gccacgaaga aggcgggcca ggccaagaag aagaaguaag gacuaguaau aaggaaacug     3540

uaugaauguc ugugggcagg aagugaagag auccuucugu aaagguuuuu ggaauuaugu     3600

cugcugaaua auaaacuuuu uugaaauaau aaaucuggua gaaaaaugag aucuaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     3720

auu                                                                   3723


<210>  2469
<211>  3729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2469
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagua ccacgugggc      240

aucgacgugg ggaccuucag cgucggccug gccgccaucg agguggacga cgccgggaug      300

cccaucaaga cccugucccu ggugagccac auccacgacu ccggccugga ccccgacgag      360

aucaagagcg ccgucacgcg gcucgcgucc agcggcaucg cccgccggac ccgccggcug      420

uaccgccgga agcgccggcg ccugcagcag cucgacaagu ucauccagcg gcaggggugg      480

cccgugaucg agcuggagga cuacuccgac ccgcuguacc ccuggaaggu gcgcgccgag      540

cucgccgcga gcuacaucgc cgacgagaag gagcggggcg agaagcuguc cgucgcccug      600

cgccacaucg cccggcaccg cggguggcgg aaccccuacg ccaaggugag cucccucuac      660

cugcccgacg gcccgagcga cgcguucaag gccauccgcg aggagaucaa gcgggccucc      720

gggcagcccg ugcccgagac cgccaccguc ggccagaugg ugacgcugug cgagcucggc      780

acccugaagc ugcgcgggga gggcggggug cucagcgccc ggcugcagca guccgacuac      840

gcgcgcgaga uccaggagau cugccgcaug caggagaucg gccaggagcu guaccggaag      900

aucaucgacg ucguguucgc cgccgagagc cccaagggcu ccgccagcuc ccgcgugggg      960

aaggacccgc uccagcccgg caagaaccgg gcccugaagg cgagcgacgc cuuccagcgc     1020

uaccggaucg ccgcccugau cgggaaccuc cgcguccggg uggacggcga gaagcgcauc     1080

cugagcgugg aggagaagaa ccuggucuuc gaccaccucg ugaaccugac ccccaagaag     1140

gagcccgagu gggugaccau cgccgagauc cuggggaucg accggggcca gcucaucggc     1200

acggcgacca ugaccgacga cggggagcgc gccggcgccc ggccgcccac ccacgacacg     1260

aaccgcucca ucgucaacag ccggaucgcg ccccuggugg acugguggaa gaccgccucc     1320

gcccuggagc agcacgccau ggucaaggcc cucagcaacg ccgaggugga cgacuucgac     1380

ucccccgagg gggcgaaggu gcaggccuuc uucgccgacc uggacgacga cguccacgcc     1440

aagcuggaca gccuccaccu cccggugggc cgcgccgcgu acuccgagga cacccuggug     1500

cggcucaccc gccggaugcu gagcgacggg gucgaccugu acacggcccg ccuccaggag     1560

uucggcaucg agcccuccug gaccccgccc acgccccgga ucggcgagcc cguggggaac     1620

cccgccgugg accgcguccu gaagaccgug agccgcuggc uggaguccgc caccaagacc     1680

uggggcgccc ccgagcgggu gaucaucgag cacguccgcg agggguucgu gaccgagaag     1740

cgggcgcgcg agauggacgg cgacaugcgg cgccgggccg cccgcaacgc caagcuguuc     1800

caggagaugc aggagaagcu gaacgugcag gggaagccga gccgggcgga ccuguggcgc     1860

uaccaguccg uccagcggca gaacugccag ugcgccuacu gcggcagccc caucaccuuc     1920

uccaacagcg agauggacca caucgugccc cgcgccggcc agggguccac gaacacccgg     1980

gagaaccucg uggccgucug ccaccgcugc aaccagagca agggcaacac cccguucgcc     2040

aucugggcga agaacaccuc caucgagggg gugagcguga aggaggccgu cgagcggacg     2100

cgccacuggg ugaccgacac cggcaugcgg agcaccgacu ucaagaaguu cacgaaggcc     2160

guggucgagc gcuuccagcg ggccaccaug gacgaggaga ucgacgcccg cuccauggag     2220

agcguggcgu ggauggccaa cgagcugcgg ucccgcgugg cccagcacuu cgccagccac     2280

ggcaccaccg uccgcgugua ccgggggucc cugacggccg aggcgcgccg ggccagcggc     2340

aucuccggga agcugaaguu cuucgacggc gugggcaaga gccgccucga ccggcgccac     2400

cacgccaucg acgccgcggu caucgccuuc accuccgacu acguggccga gacccuggcc     2460

gugcggagca accugaagca gucccaggcc caccgccagg aggcgcccca guggcgggag     2520

uucaccggga aggacgccga gcaccgcgcc gccuggcggg ucuggugcca gaagauggag     2580

aagcucagcg cccugcugac cgaggaccuc cgcgacgacc gggugguggu cauguccaac     2640

gugcgccugc ggcugggcaa cgggagcgcg cacaaggaga cgaucggcaa gcucuccaag     2700

gugaagcuga gcucccagcu gagcgucagc gacaucgaca aggccuccag cgaggcccuc     2760

uggugcgccc ugacccgcga gcccggguuc gaccccaagg agggccugcc ggccaacccc     2820

gagcggcaca uccgcgugaa cgggacccac guguacgcgg gcgacaacau cggccucuuc     2880

cccgucuccg ccgggagcau cgcccugcgg ggcggguacg ccgagcuggg cuccagcuuc     2940

caccacgccc gcguguacaa gaucaccucc gggaagaagc ccgcguucgc caugcuccgc     3000

guguacacga ucgaccugcu gccguaccgg aaccaggacc ucuucagcgu cgagcugaag     3060

ccccagacca uguccaugcg ccaggccgag aagaagcugc gggacgcccu cgcgaccggc     3120

aacgccgagu accugggcug gcugguggug gacgacgagc ucgucgugga caccagcaag     3180

aucgccacgg accaggugaa ggccgucgag gccgagcugg ggaccauccg ccgguggcgc     3240

guggacggcu ucuucucccc cagcaagcug cggcuccgcc cccugcagau guccaaggag     3300

gggaucaaga aggagagcgc gccggagcug uccaagauca ucgaccggcc cggcuggcuc     3360

cccgccguca acaagcuguu cagcgacggg aacgugaccg ugguccgccg ggacucccug     3420

ggccgcgugc ggcucgagag caccgcccac cuccccguga cguggaaggu ccagaagcgc     3480

ccggccgcca ccaagaaggc gggccaggcc aagaagaaga aguaaggacu aguaauaagg     3540

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa     3600

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaagaauu                                                             3729


<210>  2470
<211>  3429
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2470
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggcccg gauccuggcc      240

uucgacaucg gcaucagcuc caucgggugg gccuucagcg agaacgacga gcucaaggac      300

ugcggcgugc gcaucuucac caagguggag aaccccaaga ccggggaguc ccuggcccug      360

ccccggcgcc ucgcgcggag cgcccgcaag cggcuggccc gccggaaggc ccgccugaac      420

caccucaagc accugaucgc gaacgaguuc aagcugaacu acgaggacua ccaguccuuc      480

gacgagagcc ucgccaaggc cuacaagggc ucccugauca gccccuacga gcugcgguuc      540

cgcgcccuca acgagcugcu guccaagcag gacuucgccc gggucauccu ccacaucgcg      600

aagcgccggg gcuacgacga caucaagaac agcgacgaca aggagaaggg ggccauccug      660

aaggccauca agcagaacga ggagaagcug gccaacuacc aguccguggg cgaguaccuc      720

uacaaggagu acuuccagaa guucaaggag aacagcaagg aguucacgaa cgugcgcaac      780

aagaaggagu ccuacgagcg gugcaucgcc cagagcuucc ugaaggacga gcugaagcuc      840

aucuucaaga agcagcgcga guucggguuc uccuucagca agaaguucga ggaggagguc      900

cuguccgugg cguucuacaa gcgggcccug aaggacuuca gccaccucgu gggcaacugc      960

uccuucuuca ccgacgagaa gcgcgccccg aagaacagcc cccuggccuu cauguucguc     1020

gcccugaccc ggaucaucaa ccuccugaac aaccugaaga acaccgaggg gauccucuac     1080

acgaaggacg accugaacgc gcugcucaac gaggugcuga agaacggcac ccugaccuac     1140

aagcagacca agaagcuccu gggccugagc gacgacuacg aguucaaggg ggagaagggc     1200

acguacuuca ucgaguucaa gaaguacaag gaguucauca aggcccucgg ggagcacaac     1260

cugucccagg acgaccugaa cgagaucgcc aaggacauca cccucaucaa ggacgagauc     1320

aagcugaaga aggcccuggc caaguacgac cucaaccaga accagaucga cagccugucc     1380

aagcuggagu ucaaggacca ccucaacauc agcuucaagg cgcugaagcu ggugacgccc     1440

cugaugcucg agggcaagaa guacgacgag gccugcaacg agcugaaccu gaaggucgcc     1500

aucaacgagg acaagaagga cuuccucccc gccuucaacg agaccuacua caaggacgag     1560

gugaccaacc cggugguccu gcgcgcgauc aaggaguacc gcaaggugcu gaacgcccuc     1620

cugaagaagu acggcaaggu gcacaagauc aacaucgagc uggcccggga ggucgggaag     1680

aaccacuccc agcgcgccaa gaucgagaag gagcagaacg agaacuacaa ggccaagaag     1740

gacgcggagc ucgagugcga gaagcugggc cugaagauca acagcaagaa cauccucaag     1800

cugcggcugu ucaaggagca gaaggaguuc ugcgccuacu ccggggagaa gaucaagauc     1860

agcgaccucc aggacgagaa gaugcuggag aucgaccaca ucuaccccua cucccgcagc     1920

uucgacgacu ccuacaugaa caaggugcug guguucacca agcagaacca ggagaagcuc     1980

aaccagaccc ccuucgaggc cuucggcaac gacagcgcca aguggcagaa gaucgagguc     2040

cuggccaaga accugcccac caagaagcag aagcggaucc ucgacaagaa cuacaaggac     2100

aaggagcaga agaacuucaa ggaccgcaac cugaacgaca cgcgguacau cgcgcgccug     2160

gugcucaacu acaccaagga cuaccuggac uuccugccgc ucuccgacga cgagaacacc     2220

aagcugaacg acacccagaa ggggagcaag gugcacgucg aggccaaguc cggcaugcug     2280

acgagcgccc uccggcacac cuggggcuuc uccgccaagg accgcaacaa ccaccuccac     2340

cacgccaucg acgcggugau caucgccuac gccaacaaca gcaucgucaa ggccuucucc     2400

gacuucaaga aggagcagga gagcaacagc gcggagcugu acgccaagaa gaucuccgag     2460

cuggacuaca agaacaagcg gaaguucuuc gagcccuuca gcggguuccg ccagaaggug     2520

cuggacaaga ucgacgagau cuucgugucc aagcccgagc ggaagaagcc cagcggcgcc     2580

cugcacgagg agaccuuccg caaggaggag gaguucuacc aguccuacgg gggcaaggag     2640

gggguccuca aggcccugga gcugggcaag auccggaagg ugaacggcaa gaucgugaag     2700

aacggggaca uguuccgcgu cgacaucuuc aagcacaaga agaccaacaa guucuacgcc     2760

gugccgaucu acacgaugga cuucgcgcuc aaggugcugc ccaacaaggc cgucgcccgg     2820

agcaagaagg gcgagaucaa ggacuggauc cugauggacg agaacuacga guucugcuuc     2880

ucccucuaca aggacagccu gauccugauc cagaccaagg acaugcagga gcccgaguuc     2940

guguacuaca acgccuucac cuccagcacc gugucccuca ucgucagcaa gcacgacaac     3000

aaguucgaga cgcuguccaa gaaccagaag auccuguuca agaacgccaa cgagaaggag     3060

gugaucgcca agagcaucgg gauccagaac cucaaggugu ucgagaagua caucgucucc     3120

gcgcugggcg aggugaccaa ggccgaguuc cgccagcggg aggacuucaa gaagaagcgc     3180

cccgccgcca ccaagaaggc ggggcaggcc aagaagaaga aguaaggacu aguaauaagg     3240

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa     3300

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu     3360

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3420

aaaagaauu                                                             3429


<210>  2471
<211>  3840
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2471
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgagcga ccuggugcuc      240

ggccuggaca ucgggaucgg cuccgugggg gucggcaucc ugaacaaggu gaccggcgag      300

aucauccaca agaacagccg gaucuucccc gccgcccagg ccgagaacaa ccucgugcgc      360

cggaccaacc gccaggggcg gcgccuggcc cggcgcaaga agcaccggcg cguccggcug      420

aaccgccucu ucgaggaguc cggccugauc acggacuuca ccaagaucag caucaaccug      480

aaccccuacc agcuccgggu gaaggggcug accgacgagc uguccaacga ggagcuguuc      540

aucgcgcuga agaacauggu gaagcaccgc ggcaucagcu accuggacga cgccuccgac      600

gacgggaaca gcuccgucgg cgacuacgcc cagaucguga aggagaacag caagcagcuc      660

gagaccaaga cgcccggcca gauccagcug gagcgguacc agaccuacgg gcagcugcgc      720

ggcgacuuca ccguggagaa ggacgggaag aagcaccggc ucaucaacgu cuucccgacc      780

uccgccuacc gcagcgaggc gcugcggauc cugcagacgc agcaggaguu caacccccag      840

aucaccgacg aguucaucaa ccgcuaccuc gagauccuga ccggcaagcg gaaguacuac      900

cacggccccg ggaacgagaa gucccgcacc gacuacggcc gcuaccggac gagcggggag      960

acccuggaca acaucuucgg cauccucauc gggaagugca ccuucuaccc cgacgaguuc     1020

cgcgccgcca aggccuccua caccgcccag gaguucaacc ugcugaacga ccucaacaac     1080

cugacggugc cgaccgagac caagaagcug agcaaggagc agaagaacca gaucaucaac     1140

uacgugaaga acgagaaggc gaugggcccc gccaagcugu ucaaguacau cgccaagcuc     1200

cuguccugcg acgucgccga caucaagggc uaccggaucg acaagagcgg gaaggccgag     1260

auccacaccu ucgaggcgua ccgcaagaug aagacgcucg agacccugga caucgagcag     1320

auggaccggg agacccugga caagcucgcc uacgugcuga cccugaacac ggagcgcgag     1380

ggcauccagg aggcccucga gcacgaguuc gccgacgggu ccuucagcca gaagcagguc     1440

gacgagcugg ugcaguuccg gaaggccaac agcuccaucu ucggcaaggg guggcacaac     1500

uucagcguga agcugaugau ggagcucauc cccgagcugu acgagaccuc cgaggagcag     1560

augaccaucc ugacccgccu cggcaagcag aagacgacca gcuccagcaa caagaccaag     1620

uacaucgacg agaagcugcu gaccgaggag aucuacaacc ccguggucgc gaaguccgug     1680

cggcaggcca ucaagaucgu gaacgccgcc aucaaggagu acggcgacuu cgacaacauc     1740

gucaucgaga uggcccgcga gacgaacgag gacgacgaga agaaggcgau ccagaagauc     1800

cagaaggcca acaaggacga gaaggacgcc gccaugcuca aggcggccaa ccaguacaac     1860

gggaaggccg agcugccgca cagcguguuc cacggccaca agcagcuggc caccaagauc     1920

cggcuguggc accagcaggg ggagcgcugc cucuacaccg gcaagaccau cuccauccac     1980

gaccugauca acaacagcaa ccaguucgag gucgaccaca uccugccccu cuccaucacg     2040

uucgacgaca gccuggccaa caaggugcug guguacgcga ccgccaacca ggagaagggg     2100

cagcggaccc ccuaccaggc ccucgacucc auggacgacg ccuggagcuu ccgcgagcug     2160

aaggccuucg uccgggaguc caagacccug agcaacaaga agaaggagua ccuccugacc     2220

gaggaggaca ucuccaaguu cgacgugcgc aagaaguuca ucgagcggaa ccugguggac     2280

acgcgcuacg cgagccgggu cgugcucaac gcccugcagg agcacuuccg cgcccacaag     2340

aucgacacca agguguccgu cgugcgcggc caguucacca gccagcugcg gcgccacugg     2400

ggcaucgaga agacccggga cacguaccac caccacgccg uggacgcccu caucaucgcg     2460

gccagcuccc agcugaaccu guggaagaag cagaagaaca cccucgucag cuacuccgag     2520

gaccagcugc uggacaucga gaccggggag cucaucagcg acgacgagua caaggagucc     2580

guguucaagg cccccuacca gcacuucgug gacacccuca agagcaagga guucgaggac     2640

uccauccugu ucagcuacca ggucgacucc aaguucaacc gcaagaucag cgacgccacg     2700

aucuacgcga cccggcaggc caaggugggc aaggacaagg ccgacgagac cuacgugcuc     2760

gggaagauca aggacaucua cacccaggac ggcuacgacg ccuucaugaa gaucuacaag     2820

aaggacaagu ccaaguuccu gauguaccgc cacgacccgc agacguucga gaaggucauc     2880

gagcccaucc uggagaacua ccccaacaag cagaucaacg agaagggcaa ggaggugccc     2940

ugcaacccgu uccucaagua caaggaggag cacggguaca uccggaagua cagcaagaag     3000

ggcaacgggc ccgagaucaa gucccugaag uacuacgaca gcaagcuggg caaccacauc     3060

gacaucaccc ccaaggacuc caacaacaag gugguccucc agagcguguc ccccuggcgc     3120

gccgacgugu acuucaacaa gaccaccggg aaguacgaga uccugggccu gaaguacgcg     3180

gaccuccagu ucgagaaggg cacggggacc uacaagauca gccaggagaa guacaacgac     3240

aucaagaaga aggagggcgu cgacagcgac uccgaguuca aguucacccu guacaagaac     3300

gaccugcucc uggugaagga caccgagacg aaggagcagc agcuguuccg guuccucagc     3360

cgcaccaugc cgaagcagaa gcacuacgug gagcugaagc ccuacgacaa gcagaaguuc     3420

gagggcgggg aggcccugau caagguccuc gggaacgugg ccaacuccgg ccagugcaag     3480

aagggccugg gcaagagcaa caucuccauc uacaaggugc ggaccgacgu ccuggggaac     3540

cagcacauca ucaagaacga gggggacaag cccaagcucg acuucaagcg ccccgccgcc     3600

accaagaagg cgggccaggc caagaagaag aaguaaggac uaguaauaag gaaacuguau     3660

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     3720

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     3780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3840


<210>  2472
<211>  4398
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2472
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaccca guucgagggc      240

uucaccaacc uguaccaggu gagcaagacg cuccgguucg agcugauccc ccaggggaag      300

acccugaagc acauccagga gcagggcuuc aucgaggagg acaaggcccg caacgaccac      360

uacaaggagc ucaagcccau caucgaccgg aucuacaaga ccuacgccga ccagugccug      420

cagcuggugc agcucgacug ggagaaccug uccgccgcca ucgacagcua ccgcaaggag      480

aagaccgagg agacgcggaa cgcgcugauc gaggagcagg ccaccuaccg caacgccauc      540

cacgacuacu ucaucgggcg gaccgacaac cucaccgacg ccaucaacaa gcgccacgcg      600

gagaucuaca agggccuguu caaggccgag cuguucaacg gcaagguccu caagcagcug      660

gggacgguga ccaccaccga gcacgagaac gcccugcucc gguccuucga caaguucacg      720

accuacuuca gcggcuucua cgagaaccgc aagaacgugu ucuccgccga ggacaucagc      780

accgccaucc cccaccggau cguccaggac aacuucccga aguucaagga gaacugccac      840

aucuucaccc gccugaucac ggcggugccc ucccugcggg agcacuucga gaacgugaag      900

aaggccaucg ggaucuucgu cagcaccucc aucgaggagg uguucagcuu ccccuucuac      960

aaccagcucc ugacccagac ccagaucgac cuguacaacc agcuccuggg cgggaucucc     1020

cgcgaggccg gcacggagaa gaucaagggc cugaacgagg ugcucaaccu ggccauccag     1080

aagaacgacg agaccgccca caucaucgcg agccugcccc accgguucau cccccucuuc     1140

aagcagaucc uguccgaccg caacacccug agcuucaucc ucgaggaguu caaguccgac     1200

gaggagguca uccagagcuu cugcaaguac aagacccugc ugcggaacga gaacgugcuc     1260

gagacggccg aggcccuguu caacgagcug aacuccaucg accucaccca caucuucauc     1320

agccacaaga agcuggagac caucuccagc gcccugugcg accacuggga cacccuccgc     1380

aacgcccugu acgagcggcg caucagcgag cugacgggga agaucaccaa guccgcgaag     1440

gagaaggugc agcggagccu caagcacgag gacaucaacc ugcaggagau caucuccgcc     1500

gccggcaagg agcugagcga ggccuucaag cagaagaccu ccgagauccu cagccacgcc     1560

cacgcggccc uggaccagcc gcugcccacc acgcucaaga agcaggagga gaaggagauc     1620

cugaaguccc agcuggacag ccugcucggg cuguaccacc uccucgacug guucgccguc     1680

gacgagucca acgaggugga ccccgaguuc agcgcccgcc ugaccggcau caagcuggag     1740

auggagccgu cccucagcuu cuacaacaag gcgcgcaacu acgccaccaa gaagcccuac     1800

uccguggaga aguucaagcu gaacuuccag augcccaccc uggccagcgg cugggacguc     1860

aacaaggaga agaacaacgg ggccauccuc uucgugaaga acggccugua cuaccugggg     1920

aucaugccca agcagaaggg ccgguacaag gcccucuccu ucgagccgac ggagaagacc     1980

agcgaggggu ucgacaagau guacuacgac uacuuccccg acgcggccaa gaugaucccc     2040

aagugcucca cccagcugaa ggccgugacc gcccacuucc agacccacac gacccccauc     2100

cugcucagca acaacuucau cgagccgcug gagaucacca aggagaucua cgaccugaac     2160

aaccccgaga aggagcccaa gaaguuccag accgccuacg cgaagaagac gggcgaccag     2220

aagggcuacc gcgaggcccu cugcaagugg aucgacuuca cccgggacuu ccuguccaag     2280

uacaccaaga ccacgagcau cgaccugagc ucccuccgcc ccagcuccca guacaaggac     2340

cugggggagu acuacgccga gcugaacccg cuccuguacc acaucagcuu ccagcggauc     2400

gccgagaagg agaucaugga cgccgucgag accggcaagc uguaccucuu ccagaucuac     2460

aacaaggacu ucgcgaaggg gcaccacggc aagcccaacc ugcacacccu guacuggacc     2520

ggccucuucu cccccgagaa ccuggccaag acgagcauca agcugaacgg gcaggccgag     2580

cuguucuacc gccccaaguc ccggaugaag cgcauggccc accggcuggg cgagaagaug     2640

cugaacaaga agcucaagga ccagaagacc ccgauccccg acacccugua ccaggagcug     2700

uacgacuacg ugaaccaccg ccucagccac gaccuguccg acgaggcgcg ggcccugcuc     2760

cccaacguga ucaccaagga ggucagccac gagaucauca aggaccgccg guucacgucc     2820

gacaaguucu ucuuccacgu gccgaucacc cugaacuacc aggccgccaa cagccccucc     2880

aaguucaacc agcgcgucaa cgccuaccug aaggagcacc ccgagacccc caucaucggg     2940

aucgaccggg gcgagcgcaa ccucaucuac aucaccguga ucgacagcac ggggaagauc     3000

cuggagcagc ggucccugaa caccauccag caguucgacu accagaagaa gcucgacaac     3060

cgcgagaagg agcggguggc ggcccgccag gccuggagcg ucgugggcac caucaaggac     3120

cugaagcagg gguaccuguc ccaggugauc cacgagaucg ucgaccucau gauccacuac     3180

caggccgugg ugguccugga gaaccugaac uucggcuuca agagcaagcg caccggcauc     3240

gccgagaagg cgguguacca gcaguucgag aagaugcuca ucgacaagcu gaacugccug     3300

gugcucaagg acuacccggc cgagaagguc gggggcgugc ugaaccccua ccagcugacg     3360

gaccaguuca ccagcuucgc caagaugggg acccaguccg gcuuccucuu cuacgugccc     3420

gcccccuaca ccagcaagau cgacccgcug acgggcuucg ucgaccccuu cguguggaag     3480

accaucaaga accacgaguc ccggaagcac uuccuggagg gguucgacuu ccuccacuac     3540

gacgugaaga ccggcgacuu cauccugcac uucaagauga accgcaaccu gagcuuccag     3600

cgggggcucc ccggcuucau gcccgccugg gacaucgucu ucgagaagaa cgagacccag     3660

uucgacgcga aggggacgcc guucaucgcc ggcaagcgca ucgugcccgu gaucgagaac     3720

caccgguuca ccggccgcua ccgggaccug uaccccgcca acgagcugau cgcccuccug     3780

gaggagaagg ggaucgucuu ccgcgacggc uccaacaucc ugcccaagcu ccuggagaac     3840

gacgacagcc acgcgaucga caccauggug gcccugaucc gguccgugcu ccagaugcgc     3900

aacagcaacg ccgccaccgg ggaggacuac aucaacuccc cgguccggga ccugaacggc     3960

gugugcuucg acagccgcuu ccagaacccc gaguggccca uggacgccga cgcgaacggg     4020

gccuaccaca ucgcccugaa gggccagcuc cugcugaacc accucaagga guccaaggac     4080

cugaagcugc agaacggcau cagcaaccag gacuggcucg ccuacaucca ggagcugcgg     4140

aacaagcgcc ccgccgcgac gaagaaggcc gggcaggcca agaagaagaa guaaggacua     4200

guaauaagga aacuguauga augucugugg gcaggaagug aagagauccu ucuguaaagg     4260

uuuuuggaau uaugucugcu gaauaauaaa cuuuuuugaa auaauaaauc ugguagaaaa     4320

augagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaagaauu                                                   4398


<210>  2473
<211>  4377
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2473
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgagcau cuaccaggag      240

uucgugaaca aguacucccu gagcaagacc cuccgguucg agcugauccc ccagggcaag      300

acccuggaga acaucaaggc ccgcgggcuc auccuggacg acgagaagcg ggccaaggac      360

uacaagaagg ccaagcagau caucgacaag uaccaccagu ucuucaucga ggagauccug      420

uccagcgugu gcaucuccga ggaccuccug cagaacuaca gcgacgucua cuucaagcug      480

aagaaguccg acgacgacaa ccuccagaag gacuucaaga gcgccaagga cacgaucaag      540

aagcagaucu ccgaguacau caaggacagc gagaaguuca agaaccuguu caaccagaac      600

cugaucgacg cgaagaaggg ccaggagucc gaccucaucc uguggcugaa gcagagcaag      660

gacaacggga ucgagcuguu caaggccaac uccgacauca ccgacaucga cgaggcccuc      720

gagaucauca agagcuucaa gggcuggacc accuacuuca agggcuucca cgagaaccgc      780

aagaacgugu acuccagcaa cgacaucccc acguccauca ucuaccggau cguggacgac      840

aaccugccca aguuccucga gaacaaggcc aaguacgaga gccugaagga caaggcgccg      900

gaggccauca acuacgagca gaucaagaag gaccuggccg aggagcucac cuucgacauc      960

gacuacaaga ccuccgaggu caaccagcgc guguucagcc uggacgaggu guucgagauc     1020

gccaacuuca acaacuaccu gaaccagagc gggaucacca aguucaacac gaucaucggc     1080

gggaaguucg ucaacggcga gaacaccaag cggaagggga ucaacgagua caucaaccuc     1140

uacucccagc agaucaacga caagacccug aagaaguaca agaugagcgu gcuguucaag     1200

cagauccucu ccgacaccga gagcaagucc uucgugaucg acaagcugga ggacgacagc     1260

gacgucguga cgaccaugca guccuucuac gagcagaucg ccgcguucaa gaccguggag     1320

gagaagucca ucaaggagac ccugucccuc cuguucgacg accugaaggc ccagaagcuc     1380

gaccugagca agaucuacuu caagaacgac aagucccuga cggaccucag ccagcagguc     1440

uucgacgacu acuccgugau cggcaccgcc gugcuggagu acaucaccca gcagaucgcc     1500

cccaagaacc uggacaaccc cagcaagaag gagcaggagc ucaucgccaa gaagaccgag     1560

aaggcgaagu accugucccu ggagacgauc aagcucgccc uggaggaguu caacaagcac     1620

cgcgacaucg acaagcagug ccgguucgag gagauccugg ccaacuucgc cgccaucccc     1680

augaucuucg acgagaucgc gcagaacaag gacaaccucg cccagaucag caucaaguac     1740

cagaaccagg gcaagaagga ccugcugcag gccuccgccg aggacgacgu caaggccauc     1800

aaggaccucc uggaccagac caacaaccug cugcacaagc ucaagaucuu ccacaucagc     1860

caguccgagg acaaggcgaa cauccuggac aaggacgagc acuucuaccu gguguucgag     1920

gagugcuacu ucgagcucgc caacaucgug ccgcuguaca acaagauccg caacuacauc     1980

acccagaagc ccuacagcga cgagaaguuc aagcugaacu ucgagaacag cacccucgcc     2040

aacggguggg acaagaacaa ggagcccgac aacacggcca uccuguucau caaggacgac     2100

aaguacuacc ugggcgucau gaacaagaag aacaacaaga ucuucgacga caaggcgauc     2160

aaggagaaca agggggaggg cuacaagaag aucguguaca agcuccugcc cggcgccaac     2220

aagaugcugc cgaagguguu cuucuccgcc aagagcauca aguucuacaa ccccuccgag     2280

gacauccucc ggauccgcaa ccacagcacc cacaccaaga acgggagccc ccagaagggc     2340

uacgagaagu ucgaguucaa caucgaggac ugccggaagu ucaucgacuu cuacaagcag     2400

agcaucucca agcaccccga guggaaggac uucggguucc gcuucagcga cacccagcgg     2460

uacaacucca ucgacgaguu cuaccgcgag gucgagaacc agggcuacaa gcugacguuc     2520

gagaacauca gcgaguccua caucgacagc guggucaacc aggggaagcu guaccucuuc     2580

cagaucuaca acaaggacuu cuccgccuac agcaagggcc ggccgaaccu gcacacccug     2640

uacuggaagg cccucuucga cgagcgcaac cugcaggacg ugguguacaa gcugaacggc     2700

gaggcggagc uguucuaccg gaagcagucc auccccaaga agaucaccca ccccgccaag     2760

gaggccaucg ccaacaagaa caaggacaac cccaagaagg agagcgucuu cgaguacgac     2820

cucaucaagg acaagcgcuu caccgaggac aaguucuucu uccacugccc gaucacgauc     2880

aacuucaagu ccagcggggc caacaaguuc aacgacgaga ucaaccugcu ccugaaggag     2940

aaggcgaacg acgugcacau ccugagcauc gaccggggcg agcgccaccu cgccuacuac     3000

acccuggugg acgggaaggg caacaucauc aagcaggaca ccuucaacau caucgggaac     3060

gaccgcauga agaccaacua ccacgacaag cuggccgcca ucgagaagga ccgggacucc     3120

gcccgcaagg acuggaagaa gaucaacaac aucaaggaga ugaaggaggg cuaccucagc     3180

caggucgugc acgagaucgc gaagcuggug aucgaguaca acgccaucgu cguguucgag     3240

gaccugaacu ucggcuucaa gcgggggcgc uucaaggugg agaagcaggu cuaccagaag     3300

cucgagaaga ugcugaucga gaagcugaac uaccucgugu ucaaggacaa cgaguucgac     3360

aagacgggcg gggugcugcg ggccuaccag cugaccgccc ccuucgagac cuucaagaag     3420

augggcaagc agaccgggau caucuacuac guccccgcgg gcuucaccuc caagaucugc     3480

cccgugacgg gcuucgugaa ccagcucuac ccgaaguacg agagcgucuc caagagccag     3540

gaguucuucu ccaaguucga caagaucugc uacaaccugg acaaggggua cuucgaguuc     3600

agcuucgacu acaagaacuu cggcgacaag gccgccaagg ggaaguggac caucgccucc     3660

uucggcagcc gccugaucaa cuuccggaac uccgacaaga accacaacug ggacacccgc     3720

gagguguacc ccaccaagga gcucgagaag cugcugaagg acuacagcau cgaguacggc     3780

cacggggagu gcaucaaggc cgcgaucugc ggcgaguccg acaagaaguu cuucgccaag     3840

cucacgagcg ugcugaacac cauccugcag augcggaacu ccaagaccgg gaccgagcuc     3900

gacuaccuga ucagccccgu cgccgacgug aacggcaacu ucuucgacuc ccgccaggcc     3960

cccaagaaca ugccgcagga cgccgacgcg aacggggccu accacaucgg ccugaagggc     4020

cucaugcugc uggggcggau caagaacaac caggagggca agaagcucaa ccuggugauc     4080

aagaacgagg aguacuucga guucguccag aaccgcaaca acaagcggcc cgccgccacg     4140

aagaaggccg ggcaggcgaa gaagaagaag uaaggacuag uaauaaggaa acuguaugaa     4200

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4260

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4377


<210>  2474
<211>  4517
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2474
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggacaa gaaguacagc      240

aucggccugg acaucgggac caacuccgug ggcugggccg ugaucaccga cgaguacaag      300

guccccagca agaaguucaa ggugcugggg aacacggacc ggcacuccau caagaagaac      360

cugaucggcg cccugcuguu cgacagcggc gagaccgccg aggccacccg ccugaagcgg      420

accgcgcgcc ggcgcuacac gcggcgcaag aaccggaucu gcuaccugca ggagaucuuc      480

uccaacgaga uggccaaggu ggacgacagc uucuuccacc gccucgagga guccuuccug      540

gucgaggagg acaagaagca cgagcggcac cccaucuucg ggaacaucgu ggacgaggug      600

gccuaccacg agaaguaccc caccaucuac caccuccgca agaagcucgu cgacagcacc      660

gacaaggccg accugcggcu gaucuaccuc gcgcuggccc acaugaucaa guuccgcggc      720

cacuuccuga ucgaggggga ccucaacccg gacaacuccg acguggacaa gcuguucauc      780

cagcuggugc agaccuacaa ccagcuguuc gaggagaacc ccaucaacgc cagcggcguc      840

gacgccaagg ccauccuguc cgcgcggcug agcaaguccc gccggcucga gaaccugauc      900

gcccagcugc ccggggagaa gaagaacggc cucuucggca accugaucgc ccugagccuc      960

gggcugacgc ccaacuucaa guccaacuuc gaccuggccg aggacgccaa gcuccagcug     1020

agcaaggaca ccuacgacga cgaccuggac aaccuccugg cgcagaucgg cgaccaguac     1080

gccgaccugu uccucgccgc caagaaccug uccgacgcca uccugcucag cgacauccug     1140

cgcgugaaca ccgagaucac caaggcgccg cuguccgcca gcaugaucaa gcgguacgac     1200

gagcaccacc aggaccucac gcugcugaag gcccucgugc gccagcagcu gcccgagaag     1260

uacaaggaga ucuucuucga ccagagcaag aacggguacg ccggcuacau cgacggcggg     1320

gccucccagg aggaguucua caaguucauc aagcccaucc uggagaagau ggacggcacc     1380

gaggagcucc uggucaagcu gaaccgggag gaccugcucc gcaagcagcg caccuucgac     1440

aacgggagca ucccccacca gauccaccug ggcgagcugc acgcgauccu ccggcgccag     1500

gaggacuucu acccguuccu gaaggacaac cgggagaaga ucgagaagau ccugaccuuc     1560

cgcauccccu acuacguggg gccccucgcc cggggcaacu cccgcuucgc cuggaugacg     1620

cgcaagagcg aggagaccau cacccccugg aacuucgagg agguggucga caagggcgcc     1680

uccgcgcaga gcuucaucga gcggaugacc aacuucgaca agaaccugcc gaacgagaag     1740

gugcugccca agcacucccu ccuguacgag uacuucacgg uguacaacga gcugaccaag     1800

gucaaguacg ugaccgaggg gaugcggaag cccgccuucc ucagcggcga gcagaagaag     1860

gccaucgugg accugcuguu caagaccaac cgcaagguca cggugaagca gcucaaggag     1920

gacuacuuca agaagaucga gugcuucgac uccgucgaga ucagcggggu ggaggaccgg     1980

uucaacgccu cccugggcac cuaccacgac cugcucaaga ucaucaagga caaggacuuc     2040

cuggacaacg aggagaacga ggacauccug gaggacaucg ugcucacccu gacccuguuc     2100

gaggaccgcg agaugaucga ggagcggcuc aagacguacg cccaccuguu cgacgacaag     2160

gucaugaagc agcugaagcg ccggcgcuac accggguggg gccggcucag ccgcaagcug     2220

aucaacggca uccgggacaa gcaguccggg aagaccaucc uggacuuccu caagagcgac     2280

ggcuucgcga accgcaacuu caugcagcug auccacgacg acucccugac cuucaaggag     2340

gacauccaga aggcccaggu gagcgggcag ggcgacuccc uccacgagca caucgccaac     2400

cuggccggga gccccgccau caagaagggc auccugcaga cggugaaggu cguggacgag     2460

cucgugaagg ucaugggccg ccacaagccg gagaacaucg ugaucgagau ggcgcgggag     2520

aaccagacca cccagaaggg gcagaagaac ucccgcgagc ggaugaagcg caucgaggag     2580

ggcaucaagg agcuggggag ccagauccug aaggagcacc ccguggagaa cacccagcuc     2640

cagaacgaga agcuguaccu guacuaccuc cagaacggcc gggacaugua cgucgaccag     2700

gagcuggaca ucaaccgccu gagcgacuac gacguggacc acaucgugcc ccaguccuuc     2760

cucaaggacg acagcaucga caacaagguc cugacgcggu ccgacaagaa ccgcggcaag     2820

agcgacaacg ugcccuccga ggaggugguc aagaagauga agaacuacug gcggcagcug     2880

cucaacgcca agcugaucac ccagcgcaag uucgacaacc ugaccaaggc cgagcgcggg     2940

ggccucagcg agcuggacaa ggccggguuc aucaagcggc agcuggugga gacccggcag     3000

aucaccaagc acguggccca gauccucgac ucccgcauga acacgaagua cgacgagaac     3060

gacaagcuga uccgggaggu caaggugauc acccucaaga gcaagcucgu guccgacuuc     3120

cgcaaggacu uccaguucua caagguccgg gagaucaaca acuaccacca cgcgcacgac     3180

gccuaccuga acgccguggu gggcaccgcc cugaucaaga aguacccgaa gcucgagagc     3240

gaguucgucu acggggacua caagguguac gacgugcgca agaugaucgc gaaguccgag     3300

caggagaucg gcaaggccac cgccaaguac uucuucuaca gcaacaucau gaacuucuuc     3360

aagacggaga ucacccuggc caacggcgag auccggaagc gcccccugau cgagaccaac     3420

ggggagaccg gcgagaucgu cugggacaag gggcgggacu ucgccacggu gcgcaaggug     3480

cucuccaugc cccaggucaa caucgugaag aagaccgagg ugcagaccgg cggguucagc     3540

aaggagucca uccugcccaa gcgcaacagc gacaagcuga ucgcgcggaa gaaggacugg     3600

gacccgaaga aguacggcgg cuucgacucc cccaccgucg ccuacagcgu gcucgugguc     3660

gccaaggugg agaagggcaa gagcaagaag cugaaguccg ugaaggagcu gcucgggauc     3720

acgaucaugg agcgcagcuc cuucgagaag aaccccaucg acuuccugga ggccaagggg     3780

uacaaggagg ucaagaagga ccugaucauc aagcucccga aguacagccu guucgagcug     3840

gagaacggcc ggaagcgcau gcucgccucc gcgggggagc ugcagaaggg caacgagcug     3900

gcccucccca gcaaguacgu gaacuuccug uaccuggccu cccacuacga gaagcucaag     3960

ggcagccccg aggacaacga gcagaagcag cuguucgugg agcagcacaa gcacuaccug     4020

gacgagauca ucgagcagau cuccgaguuc agcaagcggg ucauccucgc cgacgccaac     4080

cuggacaagg ugcuguccgc guacaacaag caccgcgaca agcccauccg ggagcaggcc     4140

gagaacauca uccaccucuu cacccugacc aaccuggggg ccccggccgc guucaaguac     4200

uucgacacca cgaucgaccg caagcgguac accagcacca aggaggugcu cgacgccacc     4260

cugauccacc aguccaucac gggccuguac gagacccgca ucgaccucag ccagcucggg     4320

ggcgacaagc ggcccgccgc caccaagaag gccgggcagg cgaagaagaa gaaguaagga     4380

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4440

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aagaauu                                                    4517


<210>  2475
<211>  5300
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2475
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaacuu caagauccug      240

cccaucgcca ucgaccucgg cgugaagaac accggggugu ucagcgccuu cuaccagaag      300

ggcaccuccc uggagcggcu ggacaacaag aacgggaagg ucuacgagcu cagcaaggac      360

uccuacacgc ugcugaugaa caaccgcacc gcccggcgcc accagcggcg cggcaucgac      420

cggaagcagc ucgugaagcg ccuguucaag cugaucugga ccgagcagcu caaccuggag      480

ugggacaagg acacccagca ggccaucagc uuccuguuca accggcgcgg cuucuccuuc      540

aucacggacg gguacagccc cgaguaccuc aacaucgugc ccgagcaggu caaggcgauc      600

cugauggaca ucuucgacga cuacaacggc gaggacgacc uggacuccua ccucaagcug      660

gccaccgagc aggagagcaa gaucuccgag aucuacaaca agcugaugca gaagauccuc      720

gaguucaagc ugaugaagcu gugcaccgac aucaaggacg acaaggugag caccaagacg      780

cucaaggaga ucaccuccua cgaguucgag cugcuggccg acuaccucgc caacuacagc      840

gagucccuga agacccagaa guucagcuac accgacaagc aggggaaccu gaaggagcuc      900

uccuacuacc accacgacaa guacaacauc caggaguucc ugaagcggca cgcgacgauc      960

aacgaccgca uccuggacac ccuccugacc gacgaccugg acaucuggaa cuucaacuuc     1020

gagaaguucg acuucgacaa gaacgaggag aagcuccaga accaggagga caaggaccac     1080

auccaggccc accuccacca cuucguguuc gccgucaaca agaucaagag cgagauggcc     1140

uccggcgggc ggcaccgcag ccaguacuuc caggagauca ccaacgugcu ggacgagaac     1200

aaccaccagg agggcuaccu gaagaacuuc ugcgagaacc ugcacaacaa gaaguacucc     1260

aaccugagcg ugaagaaccu cgucaaccug aucggcaacc ugagcaaccu cgagcugaag     1320

ccgcugcgga aguacuucaa cgacaagauc cacgccaagg cggaccacug ggacgagcag     1380

aaguucacgg agaccuacug ccacuggauc cucggggagu ggcgcguggg cgugaaggac     1440

caggacaaga aggacggggc caaguacucc uacaaggacc ugugcaacga gcugaagcag     1500

aaggucacca aggccggccu cguggacuuc cugcuggagc uggaccccug ccggaccauc     1560

ccgcccuacc ucgacaacaa caaccgcaag ccgcccaagu gccagagccu gauccugaac     1620

cccaaguucc ucgacaacca guaccccaac uggcagcagu accugcagga gcugaagaag     1680

cuccagucca uccagaacua ccuggacagc uucgagacgg accugaaggu gcucaagucc     1740

agcaaggacc agcccuacuu cgucgaguac aaguccagca accagcagau cgccuccggc     1800

cagcgggacu acaaggaccu ggacgcccgc auccugcagu ucaucuucga ccgggugaag     1860

gcgagcgacg agcuccugcu gaacgagauc uacuuccagg ccaagaagcu caagcagaag     1920

gccuccagcg agcuggagaa gcuggagucc agcaagaagc ucgacgaggu gaucgccaac     1980

ucccagcuga gccagauccu gaagucccag cacaccaacg ggaucuucga gcagggcacc     2040

uuccuccacc uggucugcaa guacuacaag cagcgccagc gcgcccggga cagccgccug     2100

uacaucaugc ccgaguaccg guacgacaag aagcuccaca aguacaacaa caccgggcgc     2160

uucgacgacg acaaccagcu gcugacguac ugcaaccaca agccccggca gaagcgcuac     2220

cagcuccuga acgaccuggc gggcgugcuc cagguguccc ccaacuuccu gaaggacaag     2280

aucgggagcg acgacgaccu guucaucagc aaguggcucg ucgagcacau ccggggcuuc     2340

aagaaggccu gcgaggacuc ccugaagauc cagaaggaca accgcggccu gcucaaccac     2400

aagaucaaca ucgcccggaa caccaagggg aagugcgaga aggagaucuu caaccugauc     2460

ugcaagaucg agggcagcga ggacaagaag gggaacuaca agcacggccu ggccuacgag     2520

cucggggugc ugcuguucgg cgagccgaac gaggccucca agcccgaguu cgaccgcaag     2580

aucaagaagu ucaacagcau cuacuccuuc gcgcagaucc agcagaucgc cuucgccgag     2640

cggaagggca acgccaacac gugcgcgguc ugcagcgccg acaacgccca ccgcaugcag     2700

cagaucaaga ucaccgagcc cguggaggac aacaaggaca agaucauccu cuccgccaag     2760

gcccagcggc ugcccgcgau cccgacccgc aucguggacg gggccgucaa gaagauggcc     2820

accauccugg ccaagaacau cguggacgac aacuggcaga acaucaagca gguccucagc     2880

gccaagcacc agcugcacau ccccaucauc accgagucca acgcguucga guucgagccc     2940

gcccuggccg acgugaaggg caagagccuc aaggaccggc gcaagaaggc ccuggagcgg     3000

aucucccccg agaacaucuu caaggacaag aacaaccgca ucaaggaguu cgccaagggg     3060

aucagcgcgu acuccggcgc caaccugacc gacggggacu ucgacggcgc caaggaggag     3120

cucgaccaca ucaucccgcg gagccacaag aaguacggca cgcugaacga cgaggccaac     3180

cugaucugcg ugacccgcgg ggacaacaag aacaagggca accgcaucuu cugccuccgg     3240

gaccuggcgg acaacuacaa gcugaagcag uucgagacca ccgacgaccu cgagaucgag     3300

aagaagaucg ccgacacgau cugggacgcc aacaagaagg acuucaaguu cgggaacuac     3360

cgcuccuuca ucaaccugac gccccaggag cagaaggccu uccggcacgc ccuguuccuc     3420

gcggacgaga accccaucaa gcaggccgug auccgcgcca ucaacaaccg gaaccgcacc     3480

uucgucaacg gcacccagcg guacuucgcc gaggugcugg ccaacaacau cuaccugcgc     3540

gcgaagaagg agaaccucaa caccgacaag aucagcuucg acuacuucgg cauccccacc     3600

aucgggaacg gccgggggau cgccgagauc cgccagcugu acgagaaggu ggacuccgac     3660

auccaggccu acgccaaggg cgacaagccg caggccagcu acagccaccu gaucgacgcg     3720

augcucgccu ucugcaucgc cgccgacgag caccggaacg acggguccau cggccuggag     3780

aucgacaaga acuacagccu guacccccuc gacaagaaca ccggcgaggu cuucaccaag     3840

gacaucuucu cccagaucaa gaucaccgac aacgaguuca gcgacaagaa gcuggugcgc     3900

aagaaggcga ucgagggguu caacacgcac cggcagauga cccgcgacgg caucuacgcc     3960

gagaacuacc ugcccauccu cauccacaag gagcugaacg aggugcggaa gggguacacc     4020

uggaagaacu ccgaggagau caagaucuuc aagggcaaga aguacgacau ccagcagcug     4080

aacaaccucg ucuacugccu gaaguucgug gacaagccga ucagcaucga cauccagauc     4140

uccacccugg aggagcuccg caacauccug acgaccaaca acaucgccgc caccgccgag     4200

uacuacuaca ucaaccugaa gacccagaag cuccacgagu acuacaucga gaacuacaac     4260

acggcgcugg gguacaagaa guacagcaag gagauggagu uccugcgguc ccucgccuac     4320

cgcagcgagc gggugaagau caaguccauc gacgacguca agcagguccu ggacaaggac     4380

agcaacuuca ucaucggcaa gaucacccug cccuucaaga aggaguggca gcgccucuac     4440

cgggaguggc agaacaccac caucaaggac gacuacgagu uccugaaguc cuucuucaac     4500

gucaagagca ucacgaagcu gcacaagaag gugcgcaagg acuucucccu ccccaucagc     4560

accaacgagg gcaaguuccu ggugaagcgc aagaccuggg acaacaacuu caucuaccag     4620

auccugaacg acuccgacag ccgggccgac gggaccaagc ccuucauccc ggccuucgac     4680

aucuccaaga acgagaucgu ggaggccauc aucgacagcu ucacgagcaa gaacaucuuc     4740

uggcucccca agaacaucga gcugcagaag guggacaaca agaacaucuu cgcgaucgac     4800

accuccaagu gguucgaggu ggagaccccc agcgaccugc gcgacaucgg caucgccacc     4860

auccaguaca agaucgacaa caacucccgg cccaaggugc gcgucaagcu ggacuacgug     4920

aucgacgacg acagcaagau caacuacuuc augaaccacu cccuccucaa gagccgguac     4980

ccggacaagg ugcuggagau ccugaagcag uccacgauca ucgaguucga gagcuccggg     5040

uucaacaaga ccaucaagga gaugcugggc augaagcugg ccgggaucua caacgagacc     5100

agcaacaaca agcgccccgc cgccaccaag aaggcgggcc aggccaagaa gaagaaguaa     5160

ggacuagucc cuguucccag agcccacuuu uuuuucuuuu uuugaaauaa aauagccugu     5220

cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5280

aaaaaaaaaa aaaaagaauu                                                 5300


<210>  2476
<211>  3572
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2476
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagcg gaacuacauc      240

cugggccucg acaucgggau caccagcgug ggcuacggga ucaucgacua cgagacccgc      300

gacgugaucg acgccggcgu ccggcuguuc aaggaggcca acguggagaa caacgagggc      360

cgccggucca agcgcggggc ccggcgccug aagcggcgcc ggcgccaccg gauccagcgc      420

gugaagaagc uccuguucga cuacaaccug cucacggacc acagcgagcu guccggcauc      480

aaccccuacg aggcccgggu caaggggcug agccagaagc ucuccgagga ggaguucagc      540

gcggcccugc ugcaccucgc caagcgccgg ggcgugcaca acgugaacga ggucgaggag      600

gacaccggga acgagcuguc caccaaggag cagaucagcc gcaacuccaa ggcccuggag      660

gagaaguacg uggcggagcu ccagcuggag cggcugaaga aggacggcga ggugcgcggc      720

agcaucaacc gguucaagac cuccgacuac gucaaggagg ccaagcagcu ccugaaggug      780

cagaaggccu accaccagcu ggaccagagc uucaucgaca cguacaucga ccuccuggag      840

acccgccgca ccuacuacga ggggcccggc gagggguccc ccuucggcug gaaggacauc      900

aaggaguggu acgagaugcu gaugggccac ugcaccuacu ucccggagga gcuccggagc      960

gugaaguacg ccuacaacgc cgaccuguac aacgcgcuga acgaccucaa caaccugguc     1020

aucacgcgcg acgagaacga gaagcuggag uacuacgaga aguuccagau caucgagaac     1080

guguucaagc agaagaagaa gcccacccuc aagcagaucg ccaaggagau ccuggugaac     1140

gaggaggaca ucaaggggua ccgggucacc uccaccggca agcccgaguu cacgaaccug     1200

aagguguacc acgacaucaa ggacaucacc gcccgcaagg agaucaucga gaacgccgag     1260

cuccuggacc agaucgccaa gauccugacc aucuaccaga gcuccgagga cauccaggag     1320

gagcucacca accugaacag cgagcugacg caggaggaga ucgagcagau cuccaaccuc     1380

aagggguaca ccggcaccca caaccugagc cugaaggcga ucaaccucau ccuggacgag     1440

cuguggcaca ccaacgacaa ccagaucgcc aucuucaacc ggcucaagcu ggugcccaag     1500

aaggucgacc ugagccagca gaaggagauc ccgacgaccc ucguggacga cuucauccug     1560

ucccccgugg ucaagcgcag cuucauccag uccaucaagg ugaucaacgc caucaucaag     1620

aaguacgggc ugcccaacga caucaucauc gagcuggccc gggagaagaa cagcaaggac     1680

gcccagaaga ugaucaacga gaugcagaag cgcaaccggc agaccaacga gcgcaucgag     1740

gagaucaucc ggaccacggg caaggagaac gcgaaguacc ucaucgagaa gaucaagcug     1800

cacgacaugc aggagggcaa gugccuguac ucccucgagg ccaucccccu ggaggaccug     1860

cucaacaacc cguucaacua cgaggucgac cacaucaucc cccgcagcgu guccuucgac     1920

aacagcuuca acaacaaggu gcuggucaag caggaggaga acuccaagaa ggggaaccgg     1980

acccccuucc aguaccugag cuccagcgac uccaagauca gcuacgagac cuucaagaag     2040

cacauccuca accuggccaa gggcaagggg cgcaucucca agaccaagaa ggaguaccug     2100

cucgaggagc gggacaucaa ccgcuucagc gugcagaagg acuucaucaa ccggaaccug     2160

guggacacgc gcuacgccac ccggggccug augaaccucc ugcgcuccua cuuccgcguc     2220

aacaaccugg acgugaaggu caagagcauc aacgggggcu ucaccuccuu ccuccggcgc     2280

aaguggaagu ucaagaagga gcggaacaag ggcuacaagc accacgccga ggacgcgcug     2340

aucaucgcca acgccgacuu caucuucaag gaguggaaga agcuggacaa ggccaagaag     2400

gugauggaga accagauguu cgaggagaag caggcggaga gcaugcccga gaucgagacc     2460

gagcaggagu acaaggagau cuucaucacg ccgcaccaga ucaagcacau caaggacuuc     2520

aaggacuaca aguacagcca ccgcguggac aagaagccca accgggagcu caucaacgac     2580

acccuguacu ccacccgcaa ggacgacaag gggaacaccc ugaucgugaa caaccucaac     2640

ggccuguacg acaaggacaa cgacaagcug aagaagcuca ucaacaagag ccccgagaag     2700

cugcugaugu accaccacga cccccagacg uaccagaagc ucaagcugau cauggagcag     2760

uacggggacg agaagaaccc gcuguacaag uacuacgagg agaccggcaa cuaccucacc     2820

aaguacucca agaaggacaa cgggcccguc aucaagaaga ucaaguacua cggcaacaag     2880

cugaacgccc accuggacau caccgacgac uaccccaaca gccggaacaa gguggugaag     2940

cucucccuga agcccuaccg cuucgacguc uaccuggaca acggcgugua caaguucgug     3000

accgucaaga accucgacgu gaucaagaag gagaacuacu acgaggugaa cagcaagugc     3060

uacgaggagg ccaagaagcu gaagaagauc uccaaccagg ccgaguucau cgccagcuuc     3120

uacaacaacg accugaucaa gaucaacggg gagcucuacc gggucaucgg cgugaacaac     3180

gaccugcuga accgcaucga ggugaacaug aucgacauca cguaccggga guaccucgag     3240

aacaugaacg acaagcgccc gccccggauc aucaagacca ucgcguccaa gacccagagc     3300

aucaagaagu acuccaccga cauccugggg aaccuguacg aggucaagag caagaagcac     3360

ccccagauca ucaagaaggg caagcgcccc gccgccacga agaaggccgg ccaggccaag     3420

aagaagaagu aaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau     3480

aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3572


<210>  2477
<211>  4640
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2477
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgcuguu caacaagugc      240

aucaucauca gcaucaaccu cgacuucucc aacaaggaga agugcaugac caagcccuac      300

agcaucggcc uggacaucgg gaccaacucc gugggcuggg ccgugaucac ggacaacuac      360

aaggucccca gcaagaagau gaaggugcug gggaacaccu ccaagaagua caucaagaag      420

aaccuccugg gcgugcugcu guucgacagc ggcaucaccg ccgaggggcg gcgccugaag      480

cggaccgccc gccggcgcua cacgcggcgc cggaaccgca uccuguaccu ccaggagauc      540

uucuccaccg agauggccac ccuggacgac gcguucuucc agcggcugga cgacagcuuc      600

cucguccccg acgacaagcg cgacuccaag uacccgaucu ucggcaaccu gguggaggag      660

aagguguacc acgacgaguu ccccaccauc uaccaccucc ggaaguaccu cgccgacagc      720

acgaagaagg ccgaccugcg ccuggucuac cucgcccugg cgcacaugau caaguaccgg      780

gggcacuucc ugaucgaggg cgaguucaac uccaagaaca acgacaucca gaagaacuuc      840

caggacuucc ucgacaccua caacgccauc uucgagagcg accugucccu ggagaacagc      900

aagcagcucg aggagaucgu gaaggacaag aucuccaagc uggagaagaa ggaccgcauc      960

cugaagcugu uccccgggga gaagaacagc ggcaucuucu ccgaguuccu gaagcugauc     1020

gugggcaacc aggccgacuu ccggaagugc uucaaccucg acgagaaggc cagccugcac     1080

uucuccaagg agagcuacga cgaggaccug gagacccucc ugggguacau cggcgacgac     1140

uacagcgacg ucuuccugaa ggccaagaag cucuacgacg cgauccugcu guccggguuc     1200

cucaccguga cggacaacga gaccgaggcg ccccugagcu ccgccaugau caagcgcuac     1260

aacgagcaca aggaggaccu ggcccuccug aaggaguaca uccggaacau cagccugaag     1320

accuacaacg agguguucaa ggacgacacc aagaacggcu acgccggcua caucgacggg     1380

aagacgaacc aggaggacuu cuacgucuac cucaagaacc ugcuggccga guucgagggc     1440

gccgacuacu uccucgagaa gaucgaccgc gaggacuucc ugcggaagca gcgcaccuuc     1500

gacaacgggu ccaucccgua ccagauccac cuccaggaga ugcgcgccau ccuggacaag     1560

caggccaagu ucuaccccuu ccucgccaag aacaaggagc ggaucgagaa gauccugacc     1620

uuccgcaucc ccuacuacgu gggcccccug gcgcggggga acagcgacuu cgccuggucc     1680

auccgcaagc ggaacgagaa gaucaccccg uggaacuucg aggacgugau cgacaaggag     1740

agcuccgccg aggccuucau caaccgcaug acgagcuucg accucuaccu gcccgaggag     1800

aagguccugc ccaagcacuc ccuccuguac gagaccuuca acguguacaa cgagcugacc     1860

aaggugcggu ucaucgccga gagcaugcgc gacuaccagu uccucgacuc caagcagaag     1920

aaggacaucg uccggcugua cuucaaggac aagcgcaagg ugaccgacaa ggacaucauc     1980

gaguaccugc acgcgaucua cggcuacgac ggcaucgagc ucaaggggau cgagaagcag     2040

uucaacagcu cccugagcac guaccacgac cugcucaaca ucaucaacga caaggaguuc     2100

cuggacgacu ccagcaacga ggccaucauc gaggagauca uccacacccu gaccaucuuc     2160

gaggaccggg agaugaucaa gcagcgccuc uccaaguucg agaacaucuu cgacaagagc     2220

gugcugaaga agcugagccg gcgccacuac accggcuggg ggaagcucuc cgccaagcug     2280

aucaacggca uccgggacga gaaguccggg aacacgaucc uggacuaccu caucgacgac     2340

ggcaucagca accgcaacuu caugcagcug auccacgacg acgcccugag cuucaagaag     2400

aagauccaga aggcgcagau caucggcgac gaggacaagg ggaacaucaa ggaggucgug     2460

aagucccucc ccggcagccc ggccaucaag aaggggaucc ugcaguccau caagaucguc     2520

gacgagcugg ugaaggugau gggcgggcgg aagcccgaga gcaucgucgu ggagauggcc     2580

cgcgagaacc aguacaccaa ccagggcaag uccaacagcc agcagcggcu caagcgccug     2640

gagaaguccc ugaaggagcu cggcagcaag auccugaagg agaacauccc cgccaagcug     2700

uccaagaucg acaacaacgc ccuccagaac gaccgccugu accuguacua ccuccagaac     2760

gggaaggaca uguacaccgg cgacgaccug gacaucgacc ggcugagcaa cuacgacauc     2820

gaccacauca ucccccaggc guuccucaag gacaacucca ucgacaacaa ggugcugguc     2880

agcuccgcca gcaaccgcgg gaaguccgac gacuucccga gccuggaggu ggugaagaag     2940

cggaagaccu ucugguacca gcuccucaag agcaagcuga ucucccagcg caaguucgac     3000

aaccugacga aggccgagcg gggcggccug cugcccgagg acaaggccgg guucauccag     3060

cgccagcucg ucgagacccg gcagaucacc aagcacgugg cccgccugcu ggacgagaag     3120

uucaacaaca agaaggacga gaacaaccgg gcggugcgca ccgucaagau caucacgcuc     3180

aagagcaccc ugguguccca guuccggaag gacuucgagc uguacaaggu gcgcgagauc     3240

aacgacuucc accacgccca cgacgccuac cucaacgccg ucaucgccag cgcgcugcug     3300

aagaaguacc ccaagcucga gcccgaguuc guguacggcg acuacccgaa guacaacucc     3360

uuccgggagc gcaagagcgc caccgagaag guguacuucu acuccaacau caugaacauc     3420

uucaagaagu ccaucagccu ggccgacggg cgggucaucg agcgcccccu gaucgaggug     3480

aacgaggaga ccggcgagag cguguggaac aaggaguccg accucgccac cguccggcgc     3540

gugcugagcu acccccaggu gaacgucgug aagaaggugg aggagcagaa ccacgggcug     3600

gaccggggca agcccaaggg ccucuucaac gcgaaccugu ccagcaagcc gaagcccaac     3660

uccaacgaga accuggucgg ggccaaggag uaccucgacc ccaagaagua cggcggguac     3720

gccggcauca gcaacuccuu cgccgugcug gugaagggga cgaucgagaa gggcgccaag     3780

aagaagauca ccaacguccu ggaguuccag ggcaucagca uccucgaccg caucaacuac     3840

cggaaggaca agcugaacuu ccugcucgag aagggguaca aggacaucga gcugaucauc     3900

gagcugccga aguacagccu cuucgagcug uccgacggca gccgccgcau gcuggcgucc     3960

auccucagca ccaacaacaa gcgcggggag auccacaagg gcaaccagau cuuccugucc     4020

cagaaguucg ugaagcugcu cuaccacgcc aagcggauca gcaacaccau caacgagaac     4080

caccggaagu acguggagaa ccacaagaag gaguucgagg agcuguucua cuacauccug     4140

gaguucaacg agaacuacgu cggggccaag aagaacggca agcuccugaa cuccgccuuc     4200

cagagcuggc agaaccacuc caucgacgag cugugcagcu ccuucaucgg ccccacgggg     4260

agcgagcgca agggccucuu cgagcugacc ucccggggga gcgccgcgga cuucgaguuc     4320

cugggcguga agaucccccg cuaccgggac uacacccccu ccagccuccu gaaggacgcc     4380

acccugaucc accaguccgu gacggggcug uacgagaccc gcaucgaccu cgccaagcug     4440

ggcgagggca agcggccggc cgccaccaag aaggcggggc aggccaagaa gaagaaguaa     4500

ggacuagucc cuguucccag agcccacuuu uuuuucuuuu uuugaaauaa aauagccugu     4560

cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaagaauu                                                 4640


<210>  2478
<211>  3716
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2478
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcguggua cgccagccug      240

auguccgccc accaccuccg ggugggcauc gacgugggga cccacagcgu cggccuggcc      300

acccugcgcg uggacgacca cgggacgccc aucgagcucc uguccgcccu gagccacauc      360

cacgacuccg gcgugggcaa ggaggggaag aaggaccacg acacccggaa gaagcucagc      420

ggcaucgcgc gccgggcccg ccggcugcug caccaccgcc ggacccagcu ccagcagcug      480

gacgaggucc ugcgcgaccu cggguucccc auccccaccc cgggcgaguu ccuggaccug      540

aacgagcaga cggaccccua ccgggugugg cgcgugcggg cccgccucgu cgaggagaag      600

cugcccgagg agcugcgggg gcccgccauc uccauggcgg ugcgccacau cgcccggcac      660

cgcggcuggc ggaacccgua cagcaaggug gagucccucc ugagccccgc cgaggagucc      720

cccuucauga aggcccugcg cgagcggauc cucgccacca ccggcgaggu ccuggacgac      780

gggaucaccc ccggccaggc gauggcccag guggcccuga cgcacaacau cagcaugcgc      840

gggccggagg gcauccucgg caagcugcac caguccgaca acgccaacga gauccgcaag      900

aucugcgccc ggcagggggu gagccccgac gucugcaagc agcugcuccg cgcgguguuc      960

aaggccgaca gcccccgggg cuccgccgug ucccgcgucg cccccgaccc gcugcccggg     1020

cagggcagcu uccggcgcgc ccccaagugc gaccccgagu uccagcgguu ccgcaucauc     1080

uccaucgugg cgaaccugcg gaucagcgag accaaggggg agaaccgccc gcucaccgcc     1140

gacgagcggc gccacguggu caccuuccug acggaggaca gccaggccga ccugaccugg     1200

guggacgugg ccgagaagcu cggcguccac cggcgcgacc ugcggggcac cgccgugcac     1260

accgacgacg gggagcgcuc cgcggcccgg ccgcccaucg acgccacgga ccgcaucaug     1320

cggcagacca agaucagcuc ccugaagacc uggugggagg aggccgacag cgagcagcgc     1380

ggcgcgauga uccgcuaccu cuacgaggac cccaccgacu ccgagugcgc cgagaucauc     1440

gccgagcugc ccgaggagga ccaggccaag cuggacagcc uccaccuccc cgccgggcgg     1500

gcggccuacu cccgcgagag ccugacggcc cuguccgacc acaugcuggc caccaccgac     1560

gaccugcacg aggcccggaa gcgccucuuc ggcgucgacg acagcugggc gccgcccgcc     1620

gaggccauca acgcccccgu ggggaacccc uccguggacc ggacccugaa gaucgucggc     1680

cgcuaccuga gcgccgugga guccaugugg ggcacgcccg aggugaucca cgucgagcac     1740

gugcgggacg gguucaccag cgagcgcaug gcggacgagc gggacaaggc caaccgccgg     1800

cgcuacaacg acaaccagga ggccaugaag aagauccagc gggacuacgg caaggagggg     1860

uacaucuccc gcggcgacau cgugcggcuc gacgcccugg agcugcaggg gugcgcgugc     1920

cuguacugcg gcaccaccau cggcuaccac acgugccagc ucgaccacau cgucccgcag     1980

gccgggcccg gcagcaacaa ccgccggggg aaccuggugg ccgugugcga gcgcugcaac     2040

cgguccaaga gcaacacccc cuucgccguc ugggcccaga agugcggcau cccccacgug     2100

ggcgugaagg aggcgaucgg gcgcguccgg ggcuggcgca agcagacccc gaacaccucc     2160

agcgaggacc ugacgcgccu caagaaggag gugaucgccc ggcugcgccg gacccaggag     2220

gaccccgaga ucgacgagcg cagcauggag uccguggccu ggauggccaa cgagcugcac     2280

caccggaucg ccgcggccua ccccgagacc accgucaugg uguaccgcgg gagcaucacg     2340

gccgccgccc ggaaggcggc cggcaucgac ucccgcauca accucaucgg ggagaagggc     2400

cggaaggacc gcaucgaccg gcgccaccac gccguggacg ccagcgucgu ggcgcugaug     2460

gaggccuccg uggccaagac ccuggccgag cggagcuccc uccgcggcga gcagcggcug     2520

accgggaagg agcagaccug gaagcaguac accggcagca cggucggggc ccgcgagcac     2580

uucgagaugu ggcggggcca caugcugcac cucaccgagc uguucaacga gcgccuggcg     2640

gaggacaagg uguacgugac ccagaacauc cggcuccgcc uguccgacgg gaacgcccac     2700

accgucaacc ccagcaagcu ggugucccac cgccucggcg acggccugac ggugcagcag     2760

aucgaccggg ccugcacccc ggcccugugg ugcgcccuca cccgcgagaa ggacuucgac     2820

gagaagaacg ggcugcccgc gcgggaggac cgcgccaucc ggguccacgg ccacgagauc     2880

aagagcuccg acuacaucca gguguucagc aagcgcaaga agaccgacuc cgaccgggac     2940

gagacgcccu ucggggccau cgccgugcgc ggcggguucg ucgagaucgg ccccagcauc     3000

caccacgccc ggaucuaccg cguggagggc aagaagccgg uguacgcgau gcugcggguc     3060

uucacccacg accuccuguc ccagcgccac ggggaccugu ucagcgccgu gaucccgccc     3120

cagagcaucu ccaugcggug cgccgagccc aagcuccgca aggccaucac caccggcaac     3180

gccacguacc uggggugggu ggucgugggc gacgagcugg agaucaacgu ggacagcuuc     3240

accaaguacg cgaucgggcg guuccucgag gacuucccca acaccacccg cuggcggauc     3300

ugcggcuacg acacgaacuc caagcugacc cugaagccca ucguccucgc cgccgagggc     3360

cuggagaacc ccagcuccgc cgugaacgag aucguggagc ugaaggggug gcgcgucgcg     3420

aucaacgugc ucaccaaggu gcaccccacc gucgugcggc gcgacgcccu gggccggccg     3480

cgcuacagcu cccgcagcaa ccugcccacg uccuggacca ucgagaagcg gcccgccgcc     3540

accaagaagg ccgggcaggc gaagaagaag aaguaaggac uagucccugu ucccagagcc     3600

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         3716


<210>  2479
<211>  4577
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2479
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaccaa gcccuacagc      240

aucggccugg acaucgggac caacuccgug ggcugggccg ugacgaccga caacuacaag      300

guccccagca agaagaugaa ggugcucggg aacaccucca agaaguacau caagaagaac      360

cugcugggcg ugcuccuguu cgacagcggc aucaccgccg aggggcggcg ccugaagcgg      420

acggcccgcc ggcgcuacac ccggcgccgg aaccgcaucc ucuaccugca ggagaucuuc      480

uccaccgaga uggccacccu ggacgacgcg uucuuccagc ggcucgacga cagcuuccug      540

guccccgacg acaagcgcga cuccaaguac ccgaucuucg gcaaccuggu ggaggagaag      600

gccuaccacg acgaguuccc cacgaucuac caccuccgga aguaccuggc cgacagcacc      660

aagaaggccg accugcgccu cguguaccug gcgcuggccc acaugaucaa guaccggggg      720

cacuuccuca ucgagggcga guucaacucc aagaacaacg acauccagaa gaacuuccag      780

gacuuccugg acaccuacaa cgccaucuuc gagagcgacc ugucccucga gaacagcaag      840

cagcuggagg agaucgucaa ggacaagauc uccaagcugg agaagaagga ccgcauccuc      900

aagcuguucc ccggggagaa gaacagcggc aucuucuccg aguuccugaa gcucaucgug      960

ggcaaccagg ccgacuuccg gaagugcuuc aaccuggacg agaaggccag ccugcacuuc     1020

uccaaggaga gcuacgacga ggaccucgag acccugcugg gguacaucgg cgacgacuac     1080

uccgacgugu uccucaaggc gaagaagcug uacgacgcca uccugcucag cggguuccug     1140

acggucaccg acaacgagac cgaggcgccc cugagcuccg ccaugaucaa gcgcuacaac     1200

gagcacaagg aggaccucgc ccugcugaag gaguacaucc ggaacaucag ccucaagacc     1260

uacaacgagg uguucaagga cgacacgaag aacggcuacg ccggcuacau cgacgggaag     1320

accaaccagg aggacuucua cguguaccug aagaagcugc ucgccgaguu cgagggcgcc     1380

gacuacuucc uggagaagau cgaccgcgag gacuuccugc ggaagcagcg caccuucgac     1440

aacgggucca ucccguacca gauccaccuc caggagaugc gcgccauccu ggacaagcag     1500

gccaaguucu accccuuccu ggcgaagaac aaggagcgga ucgagaagau ccugaccuuc     1560

cgcauccccu acuacgucgg cccccucgcc cgggggaaca gcgacuucgc cugguccauc     1620

cgcaagcgga acgagaagau cacgccgugg aacuucgagg acgugaucga caaggagagc     1680

uccgccgagg ccuucaucaa ccgcaugacc agcuucgacc uguaccugcc cgaggagaag     1740

gugcucccca agcacucccu gcuguacgag accuucaacg ucuacaacga gcucaccaag     1800

gugcgguuca ucgcggagag caugcgcgac uaccaguucc uggacuccaa gcagaagaag     1860

gacaucgugc ggcuguacuu caaggacaag cgcaagguca cggacaagga caucaucgag     1920

uaccuccacg ccaucuacgg cuacgacggc aucgagcuga aggggaucga gaagcaguuc     1980

aacagcuccc ugagcaccua ccacgaccuc cugaacauca ucaacgacaa ggaguuccug     2040

gacgacucca gcaacgaggc caucaucgag gagaucaucc acacccucac caucuucgag     2100

gaccgggaga ugaucaagca gcgccugucc aaguucgaga acaucuucga caagagcgug     2160

cugaagaagc ucucccggcg ccacuacacg ggcuggggga agcugagcgc caagcugauc     2220

aacggcaucc gggacgagaa guccgggaac accauccucg acuaccugau cgacgacggc     2280

aucagcaacc gcaacuucau gcagcugauc cacgacgacg cgcucagcuu caagaagaag     2340

auccagaagg cccagaucau cggcgacgag gacaagggga acaucaagga gguggucaag     2400

ucccugcccg gcagcccggc caucaagaag gggauccugc aguccaucaa gaucguggac     2460

gagcucguca aggugauggg cgggcggaag cccgagagca ucguggucga gauggcccgc     2520

gagaaccagu acaccaacca gggcaagucc aacagccagc agcggcugaa gcgccuggag     2580

aagucccuca aggagcuggg cagcaagauc cugaaggaga acauccccgc caagcucucc     2640

aagaucgaca acaacgcgcu gcagaacgac cgccuguacc ucuacuaccu gcagaacggg     2700

aaggacaugu acaccggcga cgaccuggac aucgaccggc ucagcaacua cgacaucgac     2760

cacaucaucc cccaggccuu ccugaaggac aacuccaucg acaacaaggu gcuggugagc     2820

uccgccagca accgcgggaa guccgacgac gucccgagcc ucgagguggu gaagaagcgg     2880

aagacguucu gguaccagcu gcucaagagc aagcugaucu cccagcgcaa guucgacaac     2940

cugaccaagg ccgagcgggg cggccugagc cccgaggaca aggccggguu cauccagcgc     3000

cagcucgucg agacccggca gaucaccaag cacguggcgc gccugcugga cgagaaguuc     3060

aacaacaaga aggacgagaa caaccgggcc gugcgcacgg ucaagaucau cacccucaag     3120

uccacccugg ugagccaguu ccggaaggac uucgagcugu acaaggugcg cgagaucaac     3180

gacuuccacc acgcccacga cgccuaccuc aacgccgucg uggcguccgc ccugcugaag     3240

aaguacccca agcucgagcc cgaguucgug uacggcgacu acccgaagua caacagcuuc     3300

cgggagcgca aguccgccac cgagaagguc uacuucuaca gcaacaucau gaacaucuuc     3360

aagaagucca ucagccuggc cgacgggcgg gugaucgagc gcccccugau cgaggugaac     3420

gaggagaccg gcgaguccgu cuggaacaag gagagcgacc ucgcgacggu gcggcgcgug     3480

cuguccuacc cccaggucaa cguggugaag aaggucgagg agcagaacca cgggcuggac     3540

cggggcaagc ccaagggccu cuucaacgcc aaccugagcu ccaagccgaa gcccaacagc     3600

aacgagaacc uggugggggc caaggaguac cucgacccca agaaguacgg cggguacgcc     3660

ggcaucucca acagcuucac cgugcugguc aaggggacca ucgagaaggg cgccaagaag     3720

aagaucacca acgugcugga guuccagggc aucuccaucc ucgaccgcau caacuaccgg     3780

aaggacaagc ugaacuuccu gcucgagaag ggguacaagg acaucgagcu gaucaucgag     3840

cugccgaagu acagccucuu cgagcugagc gacggcuccc gccgcaugcu ggcgagcauc     3900

cucuccacga acaacaagcg cggggagauc cacaagggca accagaucuu ccugagccag     3960

aaguucguga agcugcucua ccacgccaag cggaucucca acaccaucaa cgagaaccac     4020

cggaaguacg ucgagaacca caagaaggag uucgaggagc uguucuacua cauccuggag     4080

uucaacgaga acuacguggg ggccaagaag aacggcaagc uccugaacag cgccuuccag     4140

uccuggcaga accacagcau cgacgagcug ugcuccagcu ucaucggccc caccgggucc     4200

gagcgcaagg gccucuucga gcugaccagc cggggguccg ccgcggacuu cgaguuccug     4260

ggcgugaaga ucccccgcua ccgggacuac acgcccagcu cccuccugaa ggacgccacc     4320

cugauccacc agagcgucac cgggcucuac gagacccgca ucgaccuggc caagcugggc     4380

gagggcaagc ggccggccgc cacgaagaag gcggggcagg ccaagaagaa gaaguaagga     4440

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4500

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aagaauu                                                    4577


<210>  2480
<211>  3659
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2480
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggccgc cuucaagccc      240

aacagcauca acuacauccu gggccucgac aucgggaucg ccuccguggg cugggccaug      300

guggagaucg acgaggagga gaaccccauc cggcugaucg accugggggu ccgcguguuc      360

gagcgggcgg aggugcccaa gaccggcgac agccucgcca uggcccgccg gcuggcccgc      420

uccguccggc gccugacccg gcgccgggcg caccgccucc ugcggacgcg ccggcugcuc      480

aagcgcgagg gcgugcugca ggccgccaac uucgacgaga acgggcugau caagagccuc      540

ccgaacaccc ccuggcagcu gcgggccgcc gcgcuggacc gcaagcucac gccccuggag      600

ugguccgccg ugcugcucca ccugaucaag caccggggcu accugagcca gcgcaagaac      660

gagggggaga ccgccgacaa ggagcucggc gcccugcuga agggggucgc cggcaacgcg      720

cacgcccucc agaccggcga cuuccggacc cccgccgagc uggcccugaa caaguucgag      780

aaggaguccg ggcacauccg caaccagcgc agcgacuacu cccacaccuu cagccggaag      840

gaccuccagg ccgagcugau ccuccuguuc gagaagcaga aggaguucgg caacccgcac      900

guguccgggg gccugaagga gggcaucgag acccugcuca ugacgcagcg ccccgcgcug      960

agcggggacg ccgugcagaa gaugcugggc cacugcaccu ucgagcccgc cgagcccaag     1020

gccgccaaga acaccuacac cgcggagcgg uucaucuggc ucacgaagcu gaacaaccug     1080

cgcauccucg agcagggguc cgagcggccg cugaccgaca ccgagcgcgc cacccugaug     1140

gacgagcccu accgcaagag caagcucacg uacgcccagg cccggaagcu gcugggccuc     1200

gaggacaccg cguucuucaa ggggcugcgg uacggcaagg acaacgccga ggccuccacc     1260

cugauggaga ugaaggccua ccacgccauc agccgcgcgc ucgagaagga gggccugaag     1320

gacaagaagu ccccgcugaa ccugagcccc gagcuccagg acgagaucgg gaccgccuuc     1380

ucccuguuca agacggacga ggacaucacc ggccggcuga aggaccgcau ccagccggag     1440

auccucgagg cccugcugaa gcacaucagc uucgacaagu ucguccagau cagccucaag     1500

gcccugcggc gcaucgugcc ccugauggag caggggaagc gguacgacga ggccugcgcg     1560

gagaucuacg gcgaccacua cgggaagaag aacaccgagg agaagaucua ccucccgccc     1620

auccccgccg acgagauccg caaccccgug guccugcggg cccuguccca ggcccgcaag     1680

gugaucaacg gcgugguccg gcgcuacggc agccccgccc gcauccacau cgagaccgcg     1740

cgggaggugg ggaaguccuu caaggaccgc aaggagaucg agaagcggca ggaggagaac     1800

cgcaaggacc gggagaaggc cgccgccaag uuccgcgagu acuuccccaa cuucgugggc     1860

gagcccaaga gcaaggacau ccucaagcug cggcuguacg agcagcagca cgggaagugc     1920

cucuacuccg gcaaggagau caaccugggg cgccugaacg agaagggcua cgucgagauc     1980

gaccacgcgc uccccuucag ccggacgugg gacgacuccu ucaacaacaa ggugcuggug     2040

cugggcagcg agaaccagaa caaggggaac cagacccccu acgaguacuu caacggcaag     2100

gacaacuccc gcgaguggca ggaguucaag gcccgggucg agaccagccg cuucccccgg     2160

uccaagaagc agcgcauccu ccugcagaag uucgacgagg acggguucaa ggagcggaac     2220

cugaacgaca cccgcuacgu gaaccgguuc cucugccagu ucgucgccga ccgcaugcgg     2280

cugacgggca agggcaagaa gcgcguguuc gccagcaacg ggcagaucac caaccugcuc     2340

cggggcuucu gggggcugcg caaggugcgc gccgagaacg accggcacca cgcgcuggac     2400

gccgucgugg uggccugcuc caccgucgcc augcagcaga agaucacccg cuucgugcgg     2460

uacaaggaga ugaacgccuu cgacggcaag acgaucgaca aggagaccgg ggaggugcuc     2520

caccagaaga cccacuuccc gcagcccugg gaguucuucg cgcaggaggu caugauccgc     2580

guguucggca agcccgacgg caagccggag uucgaggagg ccgacacccu ggagaagcug     2640

cggacgcucc uggccgagaa gcugagcucc cgccccgagg ccgugcacga guacgucacg     2700

ccccucuucg ugagccgggc ccccaaccgc aagauguccg ggcagggcca cauggagacc     2760

gucaagagcg cgaagcggcu ggacgagggg guguccgugc ugcgcgugcc gcucacccag     2820

cugaagcuga aggaccucga gaagaugguc aaccgggagc gcgagcccaa gcuguacgag     2880

gcccugaagg cccggcucga ggcccacaag gacgaccccg cgaaggccuu cgccgagccc     2940

uucuacaagu acgacaaggc cggcaaccgc acccagcagg ucaaggccgu gcggguggag     3000

cagguccaga agaccggggu gugggugcgc aaccacaacg gcaucgcgga caacgccacc     3060

auggugcggg uggacgucuu cgagaagggc gacaaguacu accuggugcc gaucuacagc     3120

uggcaggucg ccaaggggau ccugcccgac cgcgccgugg ugcagggcaa ggacgaggag     3180

gacuggcagc ucaucgacga cagcuucaac uucaaguucu cccugcaccc caacgaccug     3240

guggagguca ucaccaagaa ggcccggaug uucggguacu ucgcgagcug ccaccgcggc     3300

accgggaaca ucaacauccg cauccacgac cucgaccaca agaucggcaa gaacggcauc     3360

cuggagggga ucggcgugaa gacggcccug uccuuccaga aguaccagau cgacgagcuc     3420

gggaaggaga uccggcccug ccgccugaag aagcggccgc ccgugcgcaa gcggcccgcc     3480

gccaccaaga aggccggcca ggcgaagaag aagaaguaag gacuaguccc uguucccaga     3540

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      3659


<210>  2481
<211>  4415
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2481
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagaa gcccuacacc      240

aucggccugg acaucgggac caacagcgug ggcugggccg ugcucacgga ccaguacgac      300

cuggucaagc ggaagaugaa gaucgccggg gacuccgaga agaagcagau caagaagaac      360

uucuggggcg ugcgccuguu cgacgagggc cagaccgccg ccgaccggcg cauggcgcgg      420

accgcccgcc ggcgcaucga gcggcgccgg aaccgcauca gcuaccucca ggggaucuuc      480

gccgaggaga uguccaagac cgacgccaac uucuucugcc ggcugagcga cuccuucuac      540

guggacaacg agaagcgcaa cagccggcac cccuucuucg cgacgaucga ggaggagguc      600

gaguaccaca agaacuaccc caccaucuac caccuccgcg aggagcuggu gaacuccagc      660

gagaaggccg accugcggcu gguguaccuc gcccuggccc acaucaucaa guaccgcggc      720

aacuuccuga ucgagggggc ccucgacacc cagaacaccu ccgucgacgg caucuacaag      780

caguucaucc agacguacaa ccagguguuc gcgagcggga ucgaggacgg cucccugaag      840

aagcuggagg acaacaagga cguggccaag auccucgucg agaaggugac ccggaaggag      900

aagcuggagc gcauccugaa gcucuacccg ggcgagaagu ccgccgggau guucgcccag      960

uucaucagcc ugaucguggg cagcaagggg aacuuccaga agcccuucga ccugaucgag     1020

aaguccgaca ucgagugcgc caaggacagc uacgaggagg accucgaguc ccugcuggcg     1080

cucaucggcg acgaguacgc cgagcuguuc gucgccgcca agaacgccua cagcgcggug     1140

gugcugucca gcaucaucac cgucgccgag accgagacga acgccaagcu cagcgccucc     1200

augaucgagc gguucgacac ccacgaggag gaccugggcg agcugaaggc cuucaucaag     1260

cuccaccucc ccaagcacua cgaggagauc uucagcaaca ccgagaagca cggguacgcg     1320

ggcuacaucg acgggaagac caagcaggcc gacuucuaca aguacaugaa gaugacgcug     1380

gagaacaucg agggcgccga cuacuucauc gccaagaucg agaaggagaa cuuccugcgc     1440

aagcagcgca ccuucgacaa cggggcgauc ccccaccagc ugcaccugga ggagcucgag     1500

gccauccugc accagcaggc caaguacuac ccguuccuga aggagaacua cgacaagauc     1560

aagucccucg ugaccuuccg gauccccuac uucgugggcc cccuggccaa cggccagagc     1620

gaguucgccu ggcugacccg caaggcggac ggggagaucc ggcccuggaa caucgaggag     1680

aaggucgacu ucggcaaguc cgccguggac uucaucgaga agaugacgaa caaggacacc     1740

uaccucccga aggagaacgu gcugcccaag cacagccugu gcuaccagaa guaccucguc     1800

uacaacgagc ugaccaaggu gcgcuacauc aacgaccagg ggaagaccuc cuacuucagc     1860

ggccaggaga aggagcagau cuucaacgac cuguucaagc agaagcggaa ggucaagaag     1920

aaggaccucg agcuguuccu gcgcaacaug ucccacgugg agagccccac gaucgagggg     1980

cuggaggacu ccuucaacag cuccuacagc accuaccacg accuccugaa ggugggcauc     2040

aagcaggaga uccuggacaa ccccgucaac accgagaugc ucgagaacau cgugaagauc     2100

cugaccgugu ucgaggacaa gcggaugauc aaggagcagc ugcagcaguu cuccgacguc     2160

cucgacggcg uggugcugaa gaagcuggag cgccggcacu acacggggug gggccgccuc     2220

agcgccaagc ugcugauggg gauccgggac aagcaguccc accucaccau ccuggacuac     2280

cugaugaacg acgacggccu caaccgcaac cugaugcagc ugaucaacga cagcaaccuc     2340

uccuucaaga gcaucaucga gaaggagcag gucaccaccg ccgacaagga cauccagagc     2400

aucguggccg accuggcggg guccccggcc aucaagaagg gcauccugca gagccucaag     2460

aucguggacg agcuggucuc cgugaugggc uacccgcccc agacgaucgu ggucgagaug     2520

gcccgggaga accagaccac cgggaagggc aagaacaaca gccgcccccg guacaagucc     2580

cuggagaagg ccaucaagga guucgggagc cagauccuca aggagcaccc caccgacaac     2640

caggagcugc gcaacaaccg gcuguaccuc uacuaccugc agaacggcaa ggacauguac     2700

acgggccagg accuggacau ccacaaccuc uccaacuacg acaucgacca caucgugccc     2760

cagagcuuca ucaccgacaa cuccaucgac aaccuggugc ugaccagcuc cgccgggaac     2820

cgcgagaagg gcgacgacgu cccgccccuc gagaucgugc ggaagcgcaa gguguucugg     2880

gagaagcugu accaggggaa ccugaugagc aagcgcaagu ucgacuaccu caccaaggcg     2940

gagcggggcg ggcugaccga ggccgacaag gcccgcuuca uccaccggca gcuggucgag     3000

acgcgccaga ucaccaagaa cguggccaac auccuccacc agcgguucaa cuacgagaag     3060

gacgaccacg gcaacaccau gaagcagguc cgcaucguga cccugaaguc cgcgcuggug     3120

agccaguucc ggaagcaguu ccagcucuac aaggugcgcg acgucaacga cuaccaccac     3180

gcccacgacg ccuaccugaa cggcguggug gccaacacgc ugcucaaggu cuacccccag     3240

cuggagcccg aguucgugua cggggacuac caccaguucg acugguucaa ggccaacaag     3300

gcgaccgcca agaagcaguu cuacaccaac aucaugcugu ucuucgccca gaaggaccgg     3360

aucaucgacg agaacggcga gauccucugg gacaagaagu accuggacac cgugaagaag     3420

gucauguccu accgccagau gaacaucgug aagaagacgg agauccagaa gggggaguuc     3480

agcaaggcca ccaucaagcc caagggcaac uccagcaagc ugaucccccg gaagaccaac     3540

ugggacccga ugaaguacgg gggccucgac agccccaaca uggccuacgc gguggucauc     3600

gaguacgcca agggcaagaa caagcuggug uucgagaaga agaucauccg cgugaccauc     3660

auggagcgga aggccuucga gaaggacgag aaggccuucc uggaggagca gggguaccgc     3720

cagcccaagg uccucgccaa gcugccgaag uacacgcugu acgagugcga ggagggccgg     3780

cgccggaugc ucgcguccgc caacgaggcc cagaagggga accagcaggu ccugcccaac     3840

caccugguga cccuccugca ccacgccgcg aacugcgagg ugagcgacgg caagucccug     3900

gacuacaucg agagcaaccg cgagauguuc gccgagcucc uggcccacgu guccgaguuc     3960

gccaagcggu acacccuggc cgaggcgaac cucaacaaga ucaaccagcu guucgagcag     4020

aacaaggagg gggacaucaa ggccaucgcc cagagcuucg uggaccugau ggccuucaac     4080

gccaugggcg cgcccgccuc cuucaaguuc uucgagacca cgaucgagcg caagcgguac     4140

aacaaccuca aggagcugcu gaacagcacc aucaucuacc aguccaucac cggccucuac     4200

gagagccgca agcgccugga cgacaagcgg cccgccgcca ccaagaaggc cgggcaggcg     4260

aagaagaaga aguaaggacu agucccuguu cccagagccc acuuuuuuuu cuuuuuuuga     4320

aauaaaauag ccugucuuuc agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                4415


<210>  2482
<211>  4448
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2482
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagaa gcccuacagc      240

aucggccugg acaucgggac caacuccgug ggcugggccg uggucaccga cgacuacaag      300

gugcccgcca agaagaugaa ggugcucggg aacacggaca agagccacau cgagaagaac      360

cugcugggcg cccuccuguu cgacuccggc aacaccgccg aggaccggcg ccugaagcgg      420

accgcgcgcc ggcgcuacac ccggcgccgg aaccgcaucc ucuaccugca ggagaucuuc      480

agcgaggaga uggggaaggu cgacgacucc uucuuccacc ggcuggagga cagcuuccuc      540

gugacggagg acaagcgcgg cgagcggcac cccaucuucg ggaaccugga ggaggaggug      600

aaguaccacg agaacuuccc gaccaucuac caccuccgcc aguaccuggc cgacaacccc      660

gagaaggucg accugcggcu gguguaccuc gcccuggccc acaucaucaa guuccgcggc      720

cacuuccuga ucgaggggaa guucgacacc cggaacaacg acgugcagcg ccucuuccag      780

gaguuccugg cggucuacga caacaccuuc gagaacucca gccugcagga gcagaacguc      840

cagguggagg agauccucac ggacaagauc uccaagagcg ccaagaagga ccgggugcug      900

aagcuguucc ccaacgagaa guccaacggc cgcuucgccg aguuccucaa gcugaucgug      960

ggcaaccagg ccgacuucaa gaagcacuuc gagcuggagg agaaggcccc gcuccaguuc     1020

agcaaggaca ccuacgagga ggagcuggag gugcugcucg cgcagaucgg ggacaacuac     1080

gccgagcugu uccuguccgc caagaagcuc uacgacagca uccugcuguc cggcauccuc     1140

accgucaccg acguggggac gaaggccccg cugagcgccu ccaugaucca gcgguacaac     1200

gagcaccaga uggaccuggc gcagcucaag caguucaucc gccagaagcu gagcgacaag     1260

uacaacgagg uguucuccga cgucagcaag gacggcuacg ccggcuacau cgacgggaag     1320

accaaccagg aggccuucua caaguaccug aagggccucc ugaacaagau cgaggggagc     1380

ggcuacuucc uggacaagau cgagcgcgag gacuuccucc ggaagcagcg caccuucgac     1440

aacgggucca ucccccacca gauccaccuc caggagaugc gggccaucau ccgccggcag     1500

gccgaguucu accccuuccu ggcggacaac caggaccgca ucgagaagcu gcugaccuuc     1560

cggauccccu acuacguggg cccgcuggcc cgcggcaaga gcgacuucgc cuggcugucc     1620

cgcaagagcg ccgacaagau cacgcccugg aacuucgacg agaucgugga caaggagucc     1680

agcgccgagg cguucaucaa ccggaugacc aacuacgacc ucuaccugcc caaccagaag     1740

guccugccca agcacucccu ccuguacgag aaguucaccg uguacaacga gcugaccaag     1800

gucaaguaca agacggagca ggggaagacc gccuucuucg acgccaacau gaagcaggag     1860

aucuucgacg gcguguucaa gguguaccgg aaggucacca aggacaagcu cauggacuuc     1920

cuggagaagg aguucgacga guuccgcauc guggaccuga ccgggcucga caaggagaac     1980

aagguguuca acgccagcua cggcacguac cacgaccugu gcaagauccu ggacaaggac     2040

uuccucgaca acuccaagaa cgagaagauc cuggaggaca ucguccugac ccucacccug     2100

uucgaggacc gggagaugau ccgcaagcgg cuggagaacu acagcgaccu ccugaccaag     2160

gagcagguca agaagcugga gcgccggcac uacacggggu ggggccgccu cuccgcggag     2220

cugauccacg gcauccggaa caaggagagc cgcaagacca uccuggacua ccucaucgac     2280

gacgggaacu ccaaccgcaa cuucaugcag cugaucaacg acgacgcccu gagcuucaag     2340

gaggagaucg ccaaggccca ggugaucggc gagaccgaca accucaacca gguggugucc     2400

gacaucgccg ggagcccggc gaucaagaag ggcauccugc agucccugaa gaucguggac     2460

gagcucguca agaucauggg gcaccagccc gagaacaucg ugguggagau ggcccgggag     2520

aaccaguuca ccaaccaggg ccgccggaac agccagcagc gccugaaggg ccugacggac     2580

uccaucaagg aguucgggag ccagauccuc aaggagcacc ccgucgagaa cagccagcug     2640

cagaacgacc ggcuguuccu cuacuaccug cagaacggcc gcgacaugua caccggggag     2700

gagcuggaca ucgacuaccu cucccaguac gacaucgacc acaucauccc ccaggccuuc     2760

aucaaggaca acagcaucga caaccgggug cugaccucca gcaaggagaa ccgcggcaag     2820

uccgacgacg ugccgagcaa ggacgucgug cggaagauga aguccuacug gagcaagcug     2880

cucuccgcca agcugaucac ccagcgcaag uucgacaacc ugacgaaggc cgagcggggc     2940

gggcucaccg acgacgacaa ggcgggcuuc aucaagcgcc agcuggugga gacccggcag     3000

aucaccaagc acgucgcccg cauccuggac gagcgguuca acaccgagac ggacgagaac     3060

aacaagaaga uccgccaggu gaagaucgug acccucaaga gcaaccuggu cuccaacuuc     3120

cggaaggagu ucgagcugua caaggugcgc gagaucaacg acuaccacca cgcccacgac     3180

gccuaccuca acgccgugau cgggaaggcg cugcugggcg ucuaccccca gcucgagccc     3240

gaguucgugu acggggacua cccccacuuc cacggccaca aggagaacaa ggccaccgcc     3300

aagaaguucu ucuacagcaa caucaugaac uucuucaaga aggacgacgu gcggaccgac     3360

aagaacggcg agaucaucug gaagaaggac gagcacaucu ccaacaucaa gaagguccug     3420

agcuaccccc aggugaacau cgugaagaag gucgaggagc agaccggggg cuucuccaag     3480

gagagcaucc ugcccaaggg gaacuccgac aagcucaucc cccgcaagac gaagaaguuc     3540

uacugggaca ccaagaagua cggcggguuc gacagcccca ucguggccua cagcauccug     3600

gugaucgcgg acaucgagaa gggcaagucc aagaagcuga agaccgucaa ggcccucgug     3660

ggcgugacga ucauggagaa gaugaccuuc gagcgggacc cggucgccuu ccuggagcgc     3720

aagggguacc gcaacgugca ggaggagaac aucaucaagc ugcccaagua cagccucuuc     3780

aagcuggaga acggccggaa gcgccugcuc gccuccgccc gggagcugca gaaggggaac     3840

gagaucgugc ugcccaacca ccucggcacc cugcuguacc acgcgaagaa cauccacaag     3900

gucgacgagc cgaagcaccu cgacuacgug gacaagcaca aggacgaguu caaggagcug     3960

cuggacgugg ucagcaacuu cuccaagaag uacacccucg ccgaggggaa ccuggagaag     4020

aucaaggagc uguacgccca gaacaacggc gaggaccuca aggagcuggc cagcuccuuc     4080

aucaaccugc ucacguucac cgccaucggc gcgcccgcca ccuucaaguu cuucgacaag     4140

aacaucgacc gcaagcggua caccagcacg accgagaucc ugaacgccac ccugauccac     4200

caguccauca ccgggcucua cgagacgcgc aucgaccuga acaagcuggg cggggacaag     4260

cggcccgccg ccaccaagaa ggcgggccag gccaagaaga agaaguaagg acuagucccu     4320

guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaagaauu                                                              4448


<210>  2483
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2483
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgcagac caccaaccug      240

agcuacaucc ucggccugga ccuggggauc gccuccgugg gcugggccgu ggucgagauc      300

aacgagaacg aggaccccau cgggcucauc gacgugggcg ugcggaucuu cgagcgcgcc      360

gaggucccca agacgggcga gagccuggcc cugucccggc gccucgcgcg gagcacccgc      420

cggcugaucc gccggcgcgc ccaccggcug cuccuggcca agcgcuuccu gaagcgggag      480

gggauccucu ccaccaucga ccuggagaag ggccugccca accaggccug ggagcuccgc      540

guggcggggc uggagcggcg ccugagcgcc aucgaguggg gcgccgugcu ccugcaccug      600

aucaagcacc ggggguaccu cuccaagcgc aagaacgaga gccagaccaa caacaaggag      660

cugggcgccc ugcucuccgg cgucgcccag aaccaccagc ugcugcagag cgacgacuac      720

cggacgccgg cggagcucgc ccugaagaag uucgccaagg aggaggggca cauccgcaac      780

cagcggggcg ccuacaccca caccuucaac cgccuggacc uccuggccga gcugaaccuc      840

cuguucgcgc agcagcacca guucgggaac ccccacugca aggagcacau ccagcaguac      900

augaccgagc ugcucaugug gcagaagccc gcccuguccg gcgaggccau ccugaagaug      960

cucggcaagu gcacgcacga gaagaacgag uucaaggccg ccaagcacac cuacagcgcg     1020

gagcgcuucg uguggcugac caagcugaac aaccuccgga uccuggagga cggggccgag     1080

cgcgcccuga acgaggagga gcggcagcuc cugaucaacc accccuacga gaaguccaag     1140

cugaccuacg cccaggugcg caagcuccug ggccugagcg agcaggccau cuucaagcac     1200

cuccgguacu ccaaggagaa cgcggagagc gccacguuca uggagcucaa ggccuggcac     1260

gccauccgca aggcgcugga gaaccagggg cugaaggaca ccuggcagga ccucgccaag     1320

aagccggacc ugcuggacga gaucggcacc gccuucuccc ucuacaagac cgacgaggac     1380

auccagcagu accugacgaa caaggucccc aacagcguga ucaacgcccu gcucgugucc     1440

cugaacuucg acaaguucau cgagcugagc cucaagagcc ugcggaagau ccugccccuc     1500

auggagcagg ggaagcgcua cgaccaggcc ugccgggaga ucuacggcca ccacuacggc     1560

gaggcgaacc agaagaccuc ccagcugcug cccgccaucc cggcccagga gauccgcaac     1620

cccgucgugc uccggacccu gagccaggcc cgcaagguga ucaacgccau cauccggcag     1680

uacggguccc ccgcgcgcgu ccacaucgag accggccggg agcugggcaa gagcuucaag     1740

gagcgccggg agauccagaa gcagcaggag gacaaccgca cgaagcggga guccgccgug     1800

cagaaguuca aggagcuguu cagcgacuuc uccagcgagc ccaaguccaa ggacauccug     1860

aaguuccgcc uguacgagca gcagcacggg aagugccucu acagcgggaa ggagaucaac     1920

auccaccgcc ugaacgagaa gggcuacgug gagaucgacc acgcccugcc guucucccgg     1980

accugggacg acagcuucaa caacaagguc cucgugcugg ccuccgagaa ccagaacaag     2040

ggcaaccaga cccccuacga guggcugcag gggaagauca acagcgagcg cuggaagaac     2100

uucguggccc ucguccuggg cucccagugc agcgcggcca agaagcagcg gcugcucacc     2160

caggugaucg acgacaacaa guucaucgac cgcaaccuga acgacacgcg guacaucgcc     2220

cgcuuccugu ccaacuacau ccaggagaac cuccugcugg ucgggaagaa caagaagaac     2280

guguucaccc ccaacggcca gaucaccgcc cuccugcgga gccgcugggg gcugaucaag     2340

gcgcgggaga acaacaaccg ccaccacgcc cucgacgcca ucguggucgc cugcgccacc     2400

cccuccaugc agcagaagau cacgcgguuc auccgcuuca aggaggugca cccguacaag     2460

aucgagaacc gguacgagau gguggaccag gagagcggcg agaucaucag cccccacuuc     2520

cccgagcccu gggcguacuu ccgccaggag gucaacaucc ggguguucga caaccacccg     2580

gacaccgugc ugaaggagau gcugcccgac cgcccccagg ccaaccacca guucguccag     2640

ccccucuucg ugucccgggc cccgacccgc aagaugagcg gccaggggca cauggagacc     2700

aucaaguccg ccaagcggcu ggccgagggc aucagcgugc ugcgcauccc ccucacgcag     2760

cugaagccca accugcucga gaacaugguc aacaaggagc gggagccggc gcuguacgcc     2820

gggcugaagg cccgccucgc cgaguucaac caggaccccg ccaaggcguu cgccaccccc     2880

uucuacaagc agggcggcca gcaggucaag gccauccgcg uggagcaggu ccagaagucc     2940

ggggugcugg ugcgggagaa caacggcgug gccgacaacg cgagcaucgu gcgcaccgac     3000

guguucauca agaacaacaa guucuuccug guccccaucu acaccuggca ggucgccaag     3060

gggauccucc cgaacaaggc caucguggcc cacaagaacg aggacgagug ggaggagaug     3120

gacgagggcg ccaaguucaa guucucccug uuccccaacg accuggugga gcucaagacg     3180

aagaaggagu acuucuucgg guacuacauc ggccuggacc gggcgaccgg caacaucagc     3240

cugaaggagc acgacgggga gaucuccaag ggcaaggacg ggguguaccg cgugggcguc     3300

aagcucgccc ugagcuucga gaaguaccag guggacgagc uggggaagaa ccggcagauc     3360

ugccgccccc agcagcggca gcccgugcgc aagcggccgg ccgccaccaa gaaggccggc     3420

caggcgaaga agaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3480

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  2484
<211>  3659
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2484
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggccgc cuucaagccc      240

aaccccauca acuacauccu gggccucgac aucgggaucg ccagcguggg cugggccaug      300

guggagaucg acgaggacga gaaccccauc ugccugaucg accugggggu ccggguguuc      360

gagcgcgcgg aggugccgaa gaccggcgac ucccucgcca uggcccggcg ccuggcccgg      420

agcguccgcc ggcugacccg ccggcgcgcg caccggcucc ugcgcgcccg gcgccugcuc      480

aagcgggagg gcgugcugca ggccgccgac uucgacgaga acgggcugau caagucccuc      540

cccaacacgc ccuggcagcu gcgcgccgcg gcccuggacc ggaagcucac gccccuggag      600

uggagcgccg ugcugcucca ccugaucaag caccgcggcu accuguccca gcggaagaac      660

gagggggaga ccgccgacaa ggagcucggc gcccugcuga agggggucgc ggacaacgcc      720

cacgcccucc agaccggcga cuuccgcacc ccggccgagc uggcccugaa caaguucgag      780

aaggagagcg gccacauccg gaaccagcgc ggggacuacu cccacaccuu cagccgcaag      840

gaccuccagg cggagcugau ccuccucuuc gagaagcaga aggaguucgg caacccccac      900

guguccgggg gccugaagga gggcaucgag acccugcuca ugacccagcg gcccgcccug      960

agcggggacg ccgugcagaa gaugcugggc cacugcacgu ucgagcccgc cgagccgaag     1020

gccgcgaaga acaccuacac cgccgagcgc uucaucuggc ucaccaagcu gaacaaccug     1080

cggauccucg agcagggguc cgagcgcccc cugacggaca ccgagcgggc cacccugaug     1140

gacgagcccu accgcaagag caagcucacc uacgcccagg cgcggaagcu gcugggccuc     1200

gaggacacgg ccuucuucaa ggggcugcgc uacggcaagg acaacgccga ggccuccacc     1260

cugauggaga ugaaggccua ccacgcgauc agccgggccc ucgagaagga gggccugaag     1320

gacaagaagu ccccgcugaa ccugagcccc gagcuccagg acgagaucgg gaccgccuuc     1380

ucccuguuca agaccgacga ggacaucacg ggccgccuga aggaccggau ccagcccgag     1440

auccucgagg cccugcugaa gcacaucagc uucgacaagu ucguccagau cucccucaag     1500

gcccugcgcc ggaucgugcc ccugauggag caggggaagc gcuacgacga ggcgugcgcc     1560

gagaucuacg gcgaccacua cgggaagaag aacaccgagg agaagaucua ccugcccccg     1620

auccccgccg acgagauccg gaaccccgug guccugcgcg cccugagcca ggcccggaag     1680

gugaucaacg gcgugguccg ccgguacggc agccccgcgc gcauccacau cgagaccgcc     1740

cgcgaggugg ggaaguccuu caaggaccgg aaggagaucg agaagcgcca ggaggagaac     1800

cggaaggacc gcgagaaggc cgccgcgaag uuccgggagu acuucccgaa cuucgugggc     1860

gagcccaaga gcaaggacau ccucaagcug cgccuguacg agcagcagca cgggaagugc     1920

cucuacuccg gcaaggagau caaccugggg cggcugaacg agaagggcua cgucgagauc     1980

gaccacgccc uccccuucag ccgcaccugg gacgacuccu ucaacaacaa ggugcuggug     2040

cugggcagcg agaaccagaa caaggggaac cagacgcccu acgaguacuu caacggcaag     2100

gacaacuccc gggaguggca ggaguucaag gcccgcgucg agaccagccg guucccgcgc     2160

uccaagaagc agcggauccu ccugcagaag uucgacgagg acggguucaa ggagcgcaac     2220

cugaacgaca cccgguacgu gaaccgcuuc cucugccagu ucgucgccga ccggaugcgc     2280

cugaccggca agggcaagaa gcggguguuc gccagcaacg ggcagaucac gaaccugcuc     2340

cgcggcuucu gggggcugcg gaaggugcgc gcggagaacg accgccacca cgcccuggac     2400

gccgucgugg uggccugcuc caccgucgcc augcagcaga agaucacccg guucgugcgc     2460

uacaaggaga ugaacgcguu cgacggcaag accaucgaca aggagacggg ggaggugcuc     2520

caccagaaga cccacuuccc ccagcccugg gaguucuucg cccaggaggu caugauccgg     2580

guguucggca agccggacgg caagcccgag uucgaggagg ccgacacccc cgagaagcug     2640

cgcacccugc ucgccgagaa gcugagcucc cggcccgagg ccgugcacga guacgucacg     2700

ccgcuguucg ugagccgcgc gcccaaccgg aagauguccg ggcagggcca cauggagacc     2760

gucaagagcg ccaagcgccu cgacgagggg guguccgugc ugcgggugcc ccugacccag     2820

cucaagcuga aggaccugga gaagaugguc aaccgcgagc gggagcccaa gcucuacgag     2880

gcccugaagg cccgccugga ggcgcacaag gacgacccgg ccaaggccuu cgccgagccc     2940

uucuacaagu acgacaaggc cggcaaccgg acccagcagg ucaaggcggu gcgcguggag     3000

cagguccaga agaccggggu gugggugcgg aaccacaacg gcaucgccga caacgccacg     3060

auggugcgcg uggacgucuu cgagaagggc gacaaguacu accucgugcc caucuacagc     3120

uggcaggucg ccaaggggau ccugcccgac cgggccgugg ugcagggcaa ggacgaggag     3180

gacuggcagc ugaucgacga cuccuucaac uucaaguuca gccuccaccc gaacgaccug     3240

guggagguca ucaccaagaa ggcgcgcaug uucggguacu ucgccagcug ccaccggggc     3300

accgggaaca ucaacauccg cauccacgac cuggaccaca agaucggcaa gaacggcauc     3360

cucgagggga ucggcgugaa gaccgcccug uccuuccaga aguaccagau cgacgagcug     3420

gggaaggaga uccgccccug ccggcucaag aagcgcccgc ccgugcggaa gcgccccgcc     3480

gccacgaaga aggcgggcca ggccaagaag aagaaguaag gacuaguccc uguucccaga     3540

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      3659


<210>  2485
<211>  3665
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2485
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaagua ccacgugggc      240

aucgacgugg ggaccuucag cgucggccug gccgccaucg agguggacga cgccgggaug      300

cccaucaaga cccugucccu ggugagccac auccacgacu ccggccugga ccccgacgag      360

aucaagagcg ccgucacgcg gcucgcgucc agcggcaucg cccgccggac ccgccggcug      420

uaccgccgga agcgccggcg ccugcagcag cucgacaagu ucauccagcg gcaggggugg      480

cccgugaucg agcuggagga cuacuccgac ccgcuguacc ccuggaaggu gcgcgccgag      540

cucgccgcga gcuacaucgc cgacgagaag gagcggggcg agaagcuguc cgucgcccug      600

cgccacaucg cccggcaccg cggguggcgg aaccccuacg ccaaggugag cucccucuac      660

cugcccgacg gcccgagcga cgcguucaag gccauccgcg aggagaucaa gcgggccucc      720

gggcagcccg ugcccgagac cgccaccguc ggccagaugg ugacgcugug cgagcucggc      780

acccugaagc ugcgcgggga gggcggggug cucagcgccc ggcugcagca guccgacuac      840

gcgcgcgaga uccaggagau cugccgcaug caggagaucg gccaggagcu guaccggaag      900

aucaucgacg ucguguucgc cgccgagagc cccaagggcu ccgccagcuc ccgcgugggg      960

aaggacccgc uccagcccgg caagaaccgg gcccugaagg cgagcgacgc cuuccagcgc     1020

uaccggaucg ccgcccugau cgggaaccuc cgcguccggg uggacggcga gaagcgcauc     1080

cugagcgugg aggagaagaa ccuggucuuc gaccaccucg ugaaccugac ccccaagaag     1140

gagcccgagu gggugaccau cgccgagauc cuggggaucg accggggcca gcucaucggc     1200

acggcgacca ugaccgacga cggggagcgc gccggcgccc ggccgcccac ccacgacacg     1260

aaccgcucca ucgucaacag ccggaucgcg ccccuggugg acugguggaa gaccgccucc     1320

gcccuggagc agcacgccau ggucaaggcc cucagcaacg ccgaggugga cgacuucgac     1380

ucccccgagg gggcgaaggu gcaggccuuc uucgccgacc uggacgacga cguccacgcc     1440

aagcuggaca gccuccaccu cccggugggc cgcgccgcgu acuccgagga cacccuggug     1500

cggcucaccc gccggaugcu gagcgacggg gucgaccugu acacggcccg ccuccaggag     1560

uucggcaucg agcccuccug gaccccgccc acgccccgga ucggcgagcc cguggggaac     1620

cccgccgugg accgcguccu gaagaccgug agccgcuggc uggaguccgc caccaagacc     1680

uggggcgccc ccgagcgggu gaucaucgag cacguccgcg agggguucgu gaccgagaag     1740

cgggcgcgcg agauggacgg cgacaugcgg cgccgggccg cccgcaacgc caagcuguuc     1800

caggagaugc aggagaagcu gaacgugcag gggaagccga gccgggcgga ccuguggcgc     1860

uaccaguccg uccagcggca gaacugccag ugcgccuacu gcggcagccc caucaccuuc     1920

uccaacagcg agauggacca caucgugccc cgcgccggcc agggguccac gaacacccgg     1980

gagaaccucg uggccgucug ccaccgcugc aaccagagca agggcaacac cccguucgcc     2040

aucugggcga agaacaccuc caucgagggg gugagcguga aggaggccgu cgagcggacg     2100

cgccacuggg ugaccgacac cggcaugcgg agcaccgacu ucaagaaguu cacgaaggcc     2160

guggucgagc gcuuccagcg ggccaccaug gacgaggaga ucgacgcccg cuccauggag     2220

agcguggcgu ggauggccaa cgagcugcgg ucccgcgugg cccagcacuu cgccagccac     2280

ggcaccaccg uccgcgugua ccgggggucc cugacggccg aggcgcgccg ggccagcggc     2340

aucuccggga agcugaaguu cuucgacggc gugggcaaga gccgccucga ccggcgccac     2400

cacgccaucg acgccgcggu caucgccuuc accuccgacu acguggccga gacccuggcc     2460

gugcggagca accugaagca gucccaggcc caccgccagg aggcgcccca guggcgggag     2520

uucaccggga aggacgccga gcaccgcgcc gccuggcggg ucuggugcca gaagauggag     2580

aagcucagcg cccugcugac cgaggaccuc cgcgacgacc gggugguggu cauguccaac     2640

gugcgccugc ggcugggcaa cgggagcgcg cacaaggaga cgaucggcaa gcucuccaag     2700

gugaagcuga gcucccagcu gagcgucagc gacaucgaca aggccuccag cgaggcccuc     2760

uggugcgccc ugacccgcga gcccggguuc gaccccaagg agggccugcc ggccaacccc     2820

gagcggcaca uccgcgugaa cgggacccac guguacgcgg gcgacaacau cggccucuuc     2880

cccgucuccg ccgggagcau cgcccugcgg ggcggguacg ccgagcuggg cuccagcuuc     2940

caccacgccc gcguguacaa gaucaccucc gggaagaagc ccgcguucgc caugcuccgc     3000

guguacacga ucgaccugcu gccguaccgg aaccaggacc ucuucagcgu cgagcugaag     3060

ccccagacca uguccaugcg ccaggccgag aagaagcugc gggacgcccu cgcgaccggc     3120

aacgccgagu accugggcug gcugguggug gacgacgagc ucgucgugga caccagcaag     3180

aucgccacgg accaggugaa ggccgucgag gccgagcugg ggaccauccg ccgguggcgc     3240

guggacggcu ucuucucccc cagcaagcug cggcuccgcc cccugcagau guccaaggag     3300

gggaucaaga aggagagcgc gccggagcug uccaagauca ucgaccggcc cggcuggcuc     3360

cccgccguca acaagcuguu cagcgacggg aacgugaccg ugguccgccg ggacucccug     3420

ggccgcgugc ggcucgagag caccgcccac cuccccguga cguggaaggu ccagaagcgc     3480

ccggccgcca ccaagaaggc gggccaggcc aagaagaaga aguaaggacu agucccuguu     3540

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

gaauu                                                                 3665


<210>  2486
<211>  3365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2486
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcggcccg gauccuggcc      240

uucgacaucg gcaucagcuc caucgggugg gccuucagcg agaacgacga gcucaaggac      300

ugcggcgugc gcaucuucac caagguggag aaccccaaga ccggggaguc ccuggcccug      360

ccccggcgcc ucgcgcggag cgcccgcaag cggcuggccc gccggaaggc ccgccugaac      420

caccucaagc accugaucgc gaacgaguuc aagcugaacu acgaggacua ccaguccuuc      480

gacgagagcc ucgccaaggc cuacaagggc ucccugauca gccccuacga gcugcgguuc      540

cgcgcccuca acgagcugcu guccaagcag gacuucgccc gggucauccu ccacaucgcg      600

aagcgccggg gcuacgacga caucaagaac agcgacgaca aggagaaggg ggccauccug      660

aaggccauca agcagaacga ggagaagcug gccaacuacc aguccguggg cgaguaccuc      720

uacaaggagu acuuccagaa guucaaggag aacagcaagg aguucacgaa cgugcgcaac      780

aagaaggagu ccuacgagcg gugcaucgcc cagagcuucc ugaaggacga gcugaagcuc      840

aucuucaaga agcagcgcga guucggguuc uccuucagca agaaguucga ggaggagguc      900

cuguccgugg cguucuacaa gcgggcccug aaggacuuca gccaccucgu gggcaacugc      960

uccuucuuca ccgacgagaa gcgcgccccg aagaacagcc cccuggccuu cauguucguc     1020

gcccugaccc ggaucaucaa ccuccugaac aaccugaaga acaccgaggg gauccucuac     1080

acgaaggacg accugaacgc gcugcucaac gaggugcuga agaacggcac ccugaccuac     1140

aagcagacca agaagcuccu gggccugagc gacgacuacg aguucaaggg ggagaagggc     1200

acguacuuca ucgaguucaa gaaguacaag gaguucauca aggcccucgg ggagcacaac     1260

cugucccagg acgaccugaa cgagaucgcc aaggacauca cccucaucaa ggacgagauc     1320

aagcugaaga aggcccuggc caaguacgac cucaaccaga accagaucga cagccugucc     1380

aagcuggagu ucaaggacca ccucaacauc agcuucaagg cgcugaagcu ggugacgccc     1440

cugaugcucg agggcaagaa guacgacgag gccugcaacg agcugaaccu gaaggucgcc     1500

aucaacgagg acaagaagga cuuccucccc gccuucaacg agaccuacua caaggacgag     1560

gugaccaacc cggugguccu gcgcgcgauc aaggaguacc gcaaggugcu gaacgcccuc     1620

cugaagaagu acggcaaggu gcacaagauc aacaucgagc uggcccggga ggucgggaag     1680

aaccacuccc agcgcgccaa gaucgagaag gagcagaacg agaacuacaa ggccaagaag     1740

gacgcggagc ucgagugcga gaagcugggc cugaagauca acagcaagaa cauccucaag     1800

cugcggcugu ucaaggagca gaaggaguuc ugcgccuacu ccggggagaa gaucaagauc     1860

agcgaccucc aggacgagaa gaugcuggag aucgaccaca ucuaccccua cucccgcagc     1920

uucgacgacu ccuacaugaa caaggugcug guguucacca agcagaacca ggagaagcuc     1980

aaccagaccc ccuucgaggc cuucggcaac gacagcgcca aguggcagaa gaucgagguc     2040

cuggccaaga accugcccac caagaagcag aagcggaucc ucgacaagaa cuacaaggac     2100

aaggagcaga agaacuucaa ggaccgcaac cugaacgaca cgcgguacau cgcgcgccug     2160

gugcucaacu acaccaagga cuaccuggac uuccugccgc ucuccgacga cgagaacacc     2220

aagcugaacg acacccagaa ggggagcaag gugcacgucg aggccaaguc cggcaugcug     2280

acgagcgccc uccggcacac cuggggcuuc uccgccaagg accgcaacaa ccaccuccac     2340

cacgccaucg acgcggugau caucgccuac gccaacaaca gcaucgucaa ggccuucucc     2400

gacuucaaga aggagcagga gagcaacagc gcggagcugu acgccaagaa gaucuccgag     2460

cuggacuaca agaacaagcg gaaguucuuc gagcccuuca gcggguuccg ccagaaggug     2520

cuggacaaga ucgacgagau cuucgugucc aagcccgagc ggaagaagcc cagcggcgcc     2580

cugcacgagg agaccuuccg caaggaggag gaguucuacc aguccuacgg gggcaaggag     2640

gggguccuca aggcccugga gcugggcaag auccggaagg ugaacggcaa gaucgugaag     2700

aacggggaca uguuccgcgu cgacaucuuc aagcacaaga agaccaacaa guucuacgcc     2760

gugccgaucu acacgaugga cuucgcgcuc aaggugcugc ccaacaaggc cgucgcccgg     2820

agcaagaagg gcgagaucaa ggacuggauc cugauggacg agaacuacga guucugcuuc     2880

ucccucuaca aggacagccu gauccugauc cagaccaagg acaugcagga gcccgaguuc     2940

guguacuaca acgccuucac cuccagcacc gugucccuca ucgucagcaa gcacgacaac     3000

aaguucgaga cgcuguccaa gaaccagaag auccuguuca agaacgccaa cgagaaggag     3060

gugaucgcca agagcaucgg gauccagaac cucaaggugu ucgagaagua caucgucucc     3120

gcgcugggcg aggugaccaa ggccgaguuc cgccagcggg aggacuucaa gaagaagcgc     3180

cccgccgcca ccaagaaggc ggggcaggcc aagaagaaga aguaaggacu agucccuguu     3240

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3300

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3360

gaauu                                                                 3365


<210>  2487
<211>  3776
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2487
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgagcga ccuggugcuc      240

ggccuggaca ucgggaucgg cuccgugggg gucggcaucc ugaacaaggu gaccggcgag      300

aucauccaca agaacagccg gaucuucccc gccgcccagg ccgagaacaa ccucgugcgc      360

cggaccaacc gccaggggcg gcgccuggcc cggcgcaaga agcaccggcg cguccggcug      420

aaccgccucu ucgaggaguc cggccugauc acggacuuca ccaagaucag caucaaccug      480

aaccccuacc agcuccgggu gaaggggcug accgacgagc uguccaacga ggagcuguuc      540

aucgcgcuga agaacauggu gaagcaccgc ggcaucagcu accuggacga cgccuccgac      600

gacgggaaca gcuccgucgg cgacuacgcc cagaucguga aggagaacag caagcagcuc      660

gagaccaaga cgcccggcca gauccagcug gagcgguacc agaccuacgg gcagcugcgc      720

ggcgacuuca ccguggagaa ggacgggaag aagcaccggc ucaucaacgu cuucccgacc      780

uccgccuacc gcagcgaggc gcugcggauc cugcagacgc agcaggaguu caacccccag      840

aucaccgacg aguucaucaa ccgcuaccuc gagauccuga ccggcaagcg gaaguacuac      900

cacggccccg ggaacgagaa gucccgcacc gacuacggcc gcuaccggac gagcggggag      960

acccuggaca acaucuucgg cauccucauc gggaagugca ccuucuaccc cgacgaguuc     1020

cgcgccgcca aggccuccua caccgcccag gaguucaacc ugcugaacga ccucaacaac     1080

cugacggugc cgaccgagac caagaagcug agcaaggagc agaagaacca gaucaucaac     1140

uacgugaaga acgagaaggc gaugggcccc gccaagcugu ucaaguacau cgccaagcuc     1200

cuguccugcg acgucgccga caucaagggc uaccggaucg acaagagcgg gaaggccgag     1260

auccacaccu ucgaggcgua ccgcaagaug aagacgcucg agacccugga caucgagcag     1320

auggaccggg agacccugga caagcucgcc uacgugcuga cccugaacac ggagcgcgag     1380

ggcauccagg aggcccucga gcacgaguuc gccgacgggu ccuucagcca gaagcagguc     1440

gacgagcugg ugcaguuccg gaaggccaac agcuccaucu ucggcaaggg guggcacaac     1500

uucagcguga agcugaugau ggagcucauc cccgagcugu acgagaccuc cgaggagcag     1560

augaccaucc ugacccgccu cggcaagcag aagacgacca gcuccagcaa caagaccaag     1620

uacaucgacg agaagcugcu gaccgaggag aucuacaacc ccguggucgc gaaguccgug     1680

cggcaggcca ucaagaucgu gaacgccgcc aucaaggagu acggcgacuu cgacaacauc     1740

gucaucgaga uggcccgcga gacgaacgag gacgacgaga agaaggcgau ccagaagauc     1800

cagaaggcca acaaggacga gaaggacgcc gccaugcuca aggcggccaa ccaguacaac     1860

gggaaggccg agcugccgca cagcguguuc cacggccaca agcagcuggc caccaagauc     1920

cggcuguggc accagcaggg ggagcgcugc cucuacaccg gcaagaccau cuccauccac     1980

gaccugauca acaacagcaa ccaguucgag gucgaccaca uccugccccu cuccaucacg     2040

uucgacgaca gccuggccaa caaggugcug guguacgcga ccgccaacca ggagaagggg     2100

cagcggaccc ccuaccaggc ccucgacucc auggacgacg ccuggagcuu ccgcgagcug     2160

aaggccuucg uccgggaguc caagacccug agcaacaaga agaaggagua ccuccugacc     2220

gaggaggaca ucuccaaguu cgacgugcgc aagaaguuca ucgagcggaa ccugguggac     2280

acgcgcuacg cgagccgggu cgugcucaac gcccugcagg agcacuuccg cgcccacaag     2340

aucgacacca agguguccgu cgugcgcggc caguucacca gccagcugcg gcgccacugg     2400

ggcaucgaga agacccggga cacguaccac caccacgccg uggacgcccu caucaucgcg     2460

gccagcuccc agcugaaccu guggaagaag cagaagaaca cccucgucag cuacuccgag     2520

gaccagcugc uggacaucga gaccggggag cucaucagcg acgacgagua caaggagucc     2580

guguucaagg cccccuacca gcacuucgug gacacccuca agagcaagga guucgaggac     2640

uccauccugu ucagcuacca ggucgacucc aaguucaacc gcaagaucag cgacgccacg     2700

aucuacgcga cccggcaggc caaggugggc aaggacaagg ccgacgagac cuacgugcuc     2760

gggaagauca aggacaucua cacccaggac ggcuacgacg ccuucaugaa gaucuacaag     2820

aaggacaagu ccaaguuccu gauguaccgc cacgacccgc agacguucga gaaggucauc     2880

gagcccaucc uggagaacua ccccaacaag cagaucaacg agaagggcaa ggaggugccc     2940

ugcaacccgu uccucaagua caaggaggag cacggguaca uccggaagua cagcaagaag     3000

ggcaacgggc ccgagaucaa gucccugaag uacuacgaca gcaagcuggg caaccacauc     3060

gacaucaccc ccaaggacuc caacaacaag gugguccucc agagcguguc ccccuggcgc     3120

gccgacgugu acuucaacaa gaccaccggg aaguacgaga uccugggccu gaaguacgcg     3180

gaccuccagu ucgagaaggg cacggggacc uacaagauca gccaggagaa guacaacgac     3240

aucaagaaga aggagggcgu cgacagcgac uccgaguuca aguucacccu guacaagaac     3300

gaccugcucc uggugaagga caccgagacg aaggagcagc agcuguuccg guuccucagc     3360

cgcaccaugc cgaagcagaa gcacuacgug gagcugaagc ccuacgacaa gcagaaguuc     3420

gagggcgggg aggcccugau caagguccuc gggaacgugg ccaacuccgg ccagugcaag     3480

aagggccugg gcaagagcaa caucuccauc uacaaggugc ggaccgacgu ccuggggaac     3540

cagcacauca ucaagaacga gggggacaag cccaagcucg acuucaagcg ccccgccgcc     3600

accaagaagg cgggccaggc caagaagaag aaguaaggac uagucccugu ucccagagcc     3660

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         3776


<210>  2488
<211>  4334
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2488
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgaccca guucgagggc      240

uucaccaacc uguaccaggu gagcaagacg cuccgguucg agcugauccc ccaggggaag      300

acccugaagc acauccagga gcagggcuuc aucgaggagg acaaggcccg caacgaccac      360

uacaaggagc ucaagcccau caucgaccgg aucuacaaga ccuacgccga ccagugccug      420

cagcuggugc agcucgacug ggagaaccug uccgccgcca ucgacagcua ccgcaaggag      480

aagaccgagg agacgcggaa cgcgcugauc gaggagcagg ccaccuaccg caacgccauc      540

cacgacuacu ucaucgggcg gaccgacaac cucaccgacg ccaucaacaa gcgccacgcg      600

gagaucuaca agggccuguu caaggccgag cuguucaacg gcaagguccu caagcagcug      660

gggacgguga ccaccaccga gcacgagaac gcccugcucc gguccuucga caaguucacg      720

accuacuuca gcggcuucua cgagaaccgc aagaacgugu ucuccgccga ggacaucagc      780

accgccaucc cccaccggau cguccaggac aacuucccga aguucaagga gaacugccac      840

aucuucaccc gccugaucac ggcggugccc ucccugcggg agcacuucga gaacgugaag      900

aaggccaucg ggaucuucgu cagcaccucc aucgaggagg uguucagcuu ccccuucuac      960

aaccagcucc ugacccagac ccagaucgac cuguacaacc agcuccuggg cgggaucucc     1020

cgcgaggccg gcacggagaa gaucaagggc cugaacgagg ugcucaaccu ggccauccag     1080

aagaacgacg agaccgccca caucaucgcg agccugcccc accgguucau cccccucuuc     1140

aagcagaucc uguccgaccg caacacccug agcuucaucc ucgaggaguu caaguccgac     1200

gaggagguca uccagagcuu cugcaaguac aagacccugc ugcggaacga gaacgugcuc     1260

gagacggccg aggcccuguu caacgagcug aacuccaucg accucaccca caucuucauc     1320

agccacaaga agcuggagac caucuccagc gcccugugcg accacuggga cacccuccgc     1380

aacgcccugu acgagcggcg caucagcgag cugacgggga agaucaccaa guccgcgaag     1440

gagaaggugc agcggagccu caagcacgag gacaucaacc ugcaggagau caucuccgcc     1500

gccggcaagg agcugagcga ggccuucaag cagaagaccu ccgagauccu cagccacgcc     1560

cacgcggccc uggaccagcc gcugcccacc acgcucaaga agcaggagga gaaggagauc     1620

cugaaguccc agcuggacag ccugcucggg cuguaccacc uccucgacug guucgccguc     1680

gacgagucca acgaggugga ccccgaguuc agcgcccgcc ugaccggcau caagcuggag     1740

auggagccgu cccucagcuu cuacaacaag gcgcgcaacu acgccaccaa gaagcccuac     1800

uccguggaga aguucaagcu gaacuuccag augcccaccc uggccagcgg cugggacguc     1860

aacaaggaga agaacaacgg ggccauccuc uucgugaaga acggccugua cuaccugggg     1920

aucaugccca agcagaaggg ccgguacaag gcccucuccu ucgagccgac ggagaagacc     1980

agcgaggggu ucgacaagau guacuacgac uacuuccccg acgcggccaa gaugaucccc     2040

aagugcucca cccagcugaa ggccgugacc gcccacuucc agacccacac gacccccauc     2100

cugcucagca acaacuucau cgagccgcug gagaucacca aggagaucua cgaccugaac     2160

aaccccgaga aggagcccaa gaaguuccag accgccuacg cgaagaagac gggcgaccag     2220

aagggcuacc gcgaggcccu cugcaagugg aucgacuuca cccgggacuu ccuguccaag     2280

uacaccaaga ccacgagcau cgaccugagc ucccuccgcc ccagcuccca guacaaggac     2340

cugggggagu acuacgccga gcugaacccg cuccuguacc acaucagcuu ccagcggauc     2400

gccgagaagg agaucaugga cgccgucgag accggcaagc uguaccucuu ccagaucuac     2460

aacaaggacu ucgcgaaggg gcaccacggc aagcccaacc ugcacacccu guacuggacc     2520

ggccucuucu cccccgagaa ccuggccaag acgagcauca agcugaacgg gcaggccgag     2580

cuguucuacc gccccaaguc ccggaugaag cgcauggccc accggcuggg cgagaagaug     2640

cugaacaaga agcucaagga ccagaagacc ccgauccccg acacccugua ccaggagcug     2700

uacgacuacg ugaaccaccg ccucagccac gaccuguccg acgaggcgcg ggcccugcuc     2760

cccaacguga ucaccaagga ggucagccac gagaucauca aggaccgccg guucacgucc     2820

gacaaguucu ucuuccacgu gccgaucacc cugaacuacc aggccgccaa cagccccucc     2880

aaguucaacc agcgcgucaa cgccuaccug aaggagcacc ccgagacccc caucaucggg     2940

aucgaccggg gcgagcgcaa ccucaucuac aucaccguga ucgacagcac ggggaagauc     3000

cuggagcagc ggucccugaa caccauccag caguucgacu accagaagaa gcucgacaac     3060

cgcgagaagg agcggguggc ggcccgccag gccuggagcg ucgugggcac caucaaggac     3120

cugaagcagg gguaccuguc ccaggugauc cacgagaucg ucgaccucau gauccacuac     3180

caggccgugg ugguccugga gaaccugaac uucggcuuca agagcaagcg caccggcauc     3240

gccgagaagg cgguguacca gcaguucgag aagaugcuca ucgacaagcu gaacugccug     3300

gugcucaagg acuacccggc cgagaagguc gggggcgugc ugaaccccua ccagcugacg     3360

gaccaguuca ccagcuucgc caagaugggg acccaguccg gcuuccucuu cuacgugccc     3420

gcccccuaca ccagcaagau cgacccgcug acgggcuucg ucgaccccuu cguguggaag     3480

accaucaaga accacgaguc ccggaagcac uuccuggagg gguucgacuu ccuccacuac     3540

gacgugaaga ccggcgacuu cauccugcac uucaagauga accgcaaccu gagcuuccag     3600

cgggggcucc ccggcuucau gcccgccugg gacaucgucu ucgagaagaa cgagacccag     3660

uucgacgcga aggggacgcc guucaucgcc ggcaagcgca ucgugcccgu gaucgagaac     3720

caccgguuca ccggccgcua ccgggaccug uaccccgcca acgagcugau cgcccuccug     3780

gaggagaagg ggaucgucuu ccgcgacggc uccaacaucc ugcccaagcu ccuggagaac     3840

gacgacagcc acgcgaucga caccauggug gcccugaucc gguccgugcu ccagaugcgc     3900

aacagcaacg ccgccaccgg ggaggacuac aucaacuccc cgguccggga ccugaacggc     3960

gugugcuucg acagccgcuu ccagaacccc gaguggccca uggacgccga cgcgaacggg     4020

gccuaccaca ucgcccugaa gggccagcuc cugcugaacc accucaagga guccaaggac     4080

cugaagcugc agaacggcau cagcaaccag gacuggcucg ccuacaucca ggagcugcgg     4140

aacaagcgcc ccgccgcgac gaagaaggcc gggcaggcca agaagaagaa guaaggacua     4200

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     4260

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaag aauu                                                       4334


<210>  2489
<211>  4313
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2489
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaga agcgcaaggu gggcauccac ggggucccgg ccgcgagcau cuaccaggag      240

uucgugaaca aguacucccu gagcaagacc cuccgguucg agcugauccc ccagggcaag      300

acccuggaga acaucaaggc ccgcgggcuc auccuggacg acgagaagcg ggccaaggac      360

uacaagaagg ccaagcagau caucgacaag uaccaccagu ucuucaucga ggagauccug      420

uccagcgugu gcaucuccga ggaccuccug cagaacuaca gcgacgucua cuucaagcug      480

aagaaguccg acgacgacaa ccuccagaag gacuucaaga gcgccaagga cacgaucaag      540

aagcagaucu ccgaguacau caaggacagc gagaaguuca agaaccuguu caaccagaac      600

cugaucgacg cgaagaaggg ccaggagucc gaccucaucc uguggcugaa gcagagcaag      660

gacaacggga ucgagcuguu caaggccaac uccgacauca ccgacaucga cgaggcccuc      720

gagaucauca agagcuucaa gggcuggacc accuacuuca agggcuucca cgagaaccgc      780

aagaacgugu acuccagcaa cgacaucccc acguccauca ucuaccggau cguggacgac      840

aaccugccca aguuccucga gaacaaggcc aaguacgaga gccugaagga caaggcgccg      900

gaggccauca acuacgagca gaucaagaag gaccuggccg aggagcucac cuucgacauc      960

gacuacaaga ccuccgaggu caaccagcgc guguucagcc uggacgaggu guucgagauc     1020

gccaacuuca acaacuaccu gaaccagagc gggaucacca aguucaacac gaucaucggc     1080

gggaaguucg ucaacggcga gaacaccaag cggaagggga ucaacgagua caucaaccuc     1140

uacucccagc agaucaacga caagacccug aagaaguaca agaugagcgu gcuguucaag     1200

cagauccucu ccgacaccga gagcaagucc uucgugaucg acaagcugga ggacgacagc     1260

gacgucguga cgaccaugca guccuucuac gagcagaucg ccgcguucaa gaccguggag     1320

gagaagucca ucaaggagac ccugucccuc cuguucgacg accugaaggc ccagaagcuc     1380

gaccugagca agaucuacuu caagaacgac aagucccuga cggaccucag ccagcagguc     1440

uucgacgacu acuccgugau cggcaccgcc gugcuggagu acaucaccca gcagaucgcc     1500

cccaagaacc uggacaaccc cagcaagaag gagcaggagc ucaucgccaa gaagaccgag     1560

aaggcgaagu accugucccu ggagacgauc aagcucgccc uggaggaguu caacaagcac     1620

cgcgacaucg acaagcagug ccgguucgag gagauccugg ccaacuucgc cgccaucccc     1680

augaucuucg acgagaucgc gcagaacaag gacaaccucg cccagaucag caucaaguac     1740

cagaaccagg gcaagaagga ccugcugcag gccuccgccg aggacgacgu caaggccauc     1800

aaggaccucc uggaccagac caacaaccug cugcacaagc ucaagaucuu ccacaucagc     1860

caguccgagg acaaggcgaa cauccuggac aaggacgagc acuucuaccu gguguucgag     1920

gagugcuacu ucgagcucgc caacaucgug ccgcuguaca acaagauccg caacuacauc     1980

acccagaagc ccuacagcga cgagaaguuc aagcugaacu ucgagaacag cacccucgcc     2040

aacggguggg acaagaacaa ggagcccgac aacacggcca uccuguucau caaggacgac     2100

aaguacuacc ugggcgucau gaacaagaag aacaacaaga ucuucgacga caaggcgauc     2160

aaggagaaca agggggaggg cuacaagaag aucguguaca agcuccugcc cggcgccaac     2220

aagaugcugc cgaagguguu cuucuccgcc aagagcauca aguucuacaa ccccuccgag     2280

gacauccucc ggauccgcaa ccacagcacc cacaccaaga acgggagccc ccagaagggc     2340

uacgagaagu ucgaguucaa caucgaggac ugccggaagu ucaucgacuu cuacaagcag     2400

agcaucucca agcaccccga guggaaggac uucggguucc gcuucagcga cacccagcgg     2460

uacaacucca ucgacgaguu cuaccgcgag gucgagaacc agggcuacaa gcugacguuc     2520

gagaacauca gcgaguccua caucgacagc guggucaacc aggggaagcu guaccucuuc     2580

cagaucuaca acaaggacuu cuccgccuac agcaagggcc ggccgaaccu gcacacccug     2640

uacuggaagg cccucuucga cgagcgcaac cugcaggacg ugguguacaa gcugaacggc     2700

gaggcggagc uguucuaccg gaagcagucc auccccaaga agaucaccca ccccgccaag     2760

gaggccaucg ccaacaagaa caaggacaac cccaagaagg agagcgucuu cgaguacgac     2820

cucaucaagg acaagcgcuu caccgaggac aaguucuucu uccacugccc gaucacgauc     2880

aacuucaagu ccagcggggc caacaaguuc aacgacgaga ucaaccugcu ccugaaggag     2940

aaggcgaacg acgugcacau ccugagcauc gaccggggcg agcgccaccu cgccuacuac     3000

acccuggugg acgggaaggg caacaucauc aagcaggaca ccuucaacau caucgggaac     3060

gaccgcauga agaccaacua ccacgacaag cuggccgcca ucgagaagga ccgggacucc     3120

gcccgcaagg acuggaagaa gaucaacaac aucaaggaga ugaaggaggg cuaccucagc     3180

caggucgugc acgagaucgc gaagcuggug aucgaguaca acgccaucgu cguguucgag     3240

gaccugaacu ucggcuucaa gcgggggcgc uucaaggugg agaagcaggu cuaccagaag     3300

cucgagaaga ugcugaucga gaagcugaac uaccucgugu ucaaggacaa cgaguucgac     3360

aagacgggcg gggugcugcg ggccuaccag cugaccgccc ccuucgagac cuucaagaag     3420

augggcaagc agaccgggau caucuacuac guccccgcgg gcuucaccuc caagaucugc     3480

cccgugacgg gcuucgugaa ccagcucuac ccgaaguacg agagcgucuc caagagccag     3540

gaguucuucu ccaaguucga caagaucugc uacaaccugg acaaggggua cuucgaguuc     3600

agcuucgacu acaagaacuu cggcgacaag gccgccaagg ggaaguggac caucgccucc     3660

uucggcagcc gccugaucaa cuuccggaac uccgacaaga accacaacug ggacacccgc     3720

gagguguacc ccaccaagga gcucgagaag cugcugaagg acuacagcau cgaguacggc     3780

cacggggagu gcaucaaggc cgcgaucugc ggcgaguccg acaagaaguu cuucgccaag     3840

cucacgagcg ugcugaacac cauccugcag augcggaacu ccaagaccgg gaccgagcuc     3900

gacuaccuga ucagccccgu cgccgacgug aacggcaacu ucuucgacuc ccgccaggcc     3960

cccaagaaca ugccgcagga cgccgacgcg aacggggccu accacaucgg ccugaagggc     4020

cucaugcugc uggggcggau caagaacaac caggagggca agaagcucaa ccuggugauc     4080

aagaacgagg aguacuucga guucguccag aaccgcaaca acaagcggcc cgccgccacg     4140

aagaaggccg ggcaggcgaa gaagaagaag uaaggacuag ucccuguucc cagagcccac     4200

uuuuuuuucu uuuuuugaaa uaaaauagcc ugucuuucag aucuaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4313


<210>  2490
<211>  4466
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2490
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcggacaag      180

aaguacagca ucggccugga caucgggacc aacuccgugg gcugggccgu gaucaccgac      240

gaguacaagg uccccagcaa gaaguucaag gugcugggga acacggaccg gcacuccauc      300

aagaagaacc ugaucggcgc ccugcuguuc gacagcggcg agaccgccga ggccacccgc      360

cugaagcgga ccgcgcgccg gcgcuacacg cggcgcaaga accggaucug cuaccugcag      420

gagaucuucu ccaacgagau ggccaaggug gacgacagcu ucuuccaccg ccucgaggag      480

uccuuccugg ucgaggagga caagaagcac gagcggcacc ccaucuucgg gaacaucgug      540

gacgaggugg ccuaccacga gaaguacccc accaucuacc accuccgcaa gaagcucguc      600

gacagcaccg acaaggccga ccugcggcug aucuaccucg cgcuggccca caugaucaag      660

uuccgcggcc acuuccugau cgagggggac cucaacccgg acaacuccga cguggacaag      720

cuguucaucc agcuggugca gaccuacaac cagcuguucg aggagaaccc caucaacgcc      780

agcggcgucg acgccaaggc cauccugucc gcgcggcuga gcaagucccg ccggcucgag      840

aaccugaucg cccagcugcc cggggagaag aagaacggcc ucuucggcaa ccugaucgcc      900

cugagccucg ggcugacgcc caacuucaag uccaacuucg accuggccga ggacgccaag      960

cuccagcuga gcaaggacac cuacgacgac gaccuggaca accuccuggc gcagaucggc     1020

gaccaguacg ccgaccuguu ccucgccgcc aagaaccugu ccgacgccau ccugcucagc     1080

gacauccugc gcgugaacac cgagaucacc aaggcgccgc uguccgccag caugaucaag     1140

cgguacgacg agcaccacca ggaccucacg cugcugaagg cccucgugcg ccagcagcug     1200

cccgagaagu acaaggagau cuucuucgac cagagcaaga acggguacgc cggcuacauc     1260

gacggcgggg ccucccagga ggaguucuac aaguucauca agcccauccu ggagaagaug     1320

gacggcaccg aggagcuccu ggucaagcug aaccgggagg accugcuccg caagcagcgc     1380

accuucgaca acgggagcau cccccaccag auccaccugg gcgagcugca cgcgauccuc     1440

cggcgccagg aggacuucua cccguuccug aaggacaacc gggagaagau cgagaagauc     1500

cugaccuucc gcauccccua cuacgugggg ccccucgccc ggggcaacuc ccgcuucgcc     1560

uggaugacgc gcaagagcga ggagaccauc acccccugga acuucgagga gguggucgac     1620

aagggcgccu ccgcgcagag cuucaucgag cggaugacca acuucgacaa gaaccugccg     1680

aacgagaagg ugcugcccaa gcacucccuc cuguacgagu acuucacggu guacaacgag     1740

cugaccaagg ucaaguacgu gaccgagggg augcggaagc ccgccuuccu cagcggcgag     1800

cagaagaagg ccaucgugga ccugcuguuc aagaccaacc gcaaggucac ggugaagcag     1860

cucaaggagg acuacuucaa gaagaucgag ugcuucgacu ccgucgagau cagcggggug     1920

gaggaccggu ucaacgccuc ccugggcacc uaccacgacc ugcucaagau caucaaggac     1980

aaggacuucc uggacaacga ggagaacgag gacauccugg aggacaucgu gcucacccug     2040

acccuguucg aggaccgcga gaugaucgag gagcggcuca agacguacgc ccaccuguuc     2100

gacgacaagg ucaugaagca gcugaagcgc cggcgcuaca ccgggugggg ccggcucagc     2160

cgcaagcuga ucaacggcau ccgggacaag caguccggga agaccauccu ggacuuccuc     2220

aagagcgacg gcuucgcgaa ccgcaacuuc augcagcuga uccacgacga cucccugacc     2280

uucaaggagg acauccagaa ggcccaggug agcgggcagg gcgacucccu ccacgagcac     2340

aucgccaacc uggccgggag ccccgccauc aagaagggca uccugcagac ggugaagguc     2400

guggacgagc ucgugaaggu caugggccgc cacaagccgg agaacaucgu gaucgagaug     2460

gcgcgggaga accagaccac ccagaagggg cagaagaacu cccgcgagcg gaugaagcgc     2520

aucgaggagg gcaucaagga gcuggggagc cagauccuga aggagcaccc cguggagaac     2580

acccagcucc agaacgagaa gcuguaccug uacuaccucc agaacggccg ggacauguac     2640

gucgaccagg agcuggacau caaccgccug agcgacuacg acguggacca caucgugccc     2700

caguccuucc ucaaggacga cagcaucgac aacaaggucc ugacgcgguc cgacaagaac     2760

cgcggcaaga gcgacaacgu gcccuccgag gaggugguca agaagaugaa gaacuacugg     2820

cggcagcugc ucaacgccaa gcugaucacc cagcgcaagu ucgacaaccu gaccaaggcc     2880

gagcgcgggg gccucagcga gcuggacaag gccggguuca ucaagcggca gcugguggag     2940

acccggcaga ucaccaagca cguggcccag auccucgacu cccgcaugaa cacgaaguac     3000

gacgagaacg acaagcugau ccgggagguc aaggugauca cccucaagag caagcucgug     3060

uccgacuucc gcaaggacuu ccaguucuac aagguccggg agaucaacaa cuaccaccac     3120

gcgcacgacg ccuaccugaa cgccguggug ggcaccgccc ugaucaagaa guacccgaag     3180

cucgagagcg aguucgucua cggggacuac aagguguacg acgugcgcaa gaugaucgcg     3240

aaguccgagc aggagaucgg caaggccacc gccaaguacu ucuucuacag caacaucaug     3300

aacuucuuca agacggagau cacccuggcc aacggcgaga uccggaagcg cccccugauc     3360

gagaccaacg gggagaccgg cgagaucguc ugggacaagg ggcgggacuu cgccacggug     3420

cgcaaggugc ucuccaugcc ccaggucaac aucgugaaga agaccgaggu gcagaccggc     3480

ggguucagca aggaguccau ccugcccaag cgcaacagcg acaagcugau cgcgcggaag     3540

aaggacuggg acccgaagaa guacggcggc uucgacuccc ccaccgucgc cuacagcgug     3600

cucguggucg ccaaggugga gaagggcaag agcaagaagc ugaaguccgu gaaggagcug     3660

cucgggauca cgaucaugga gcgcagcucc uucgagaaga accccaucga cuuccuggag     3720

gccaaggggu acaaggaggu caagaaggac cugaucauca agcucccgaa guacagccug     3780

uucgagcugg agaacggccg gaagcgcaug cucgccuccg cgggggagcu gcagaagggc     3840

aacgagcugg cccuccccag caaguacgug aacuuccugu accuggccuc ccacuacgag     3900

aagcucaagg gcagccccga ggacaacgag cagaagcagc uguucgugga gcagcacaag     3960

cacuaccugg acgagaucau cgagcagauc uccgaguuca gcaagcgggu cauccucgcc     4020

gacgccaacc uggacaaggu gcuguccgcg uacaacaagc accgcgacaa gcccauccgg     4080

gagcaggccg agaacaucau ccaccucuuc acccugacca accugggggc cccggccgcg     4140

uucaaguacu ucgacaccac gaucgaccgc aagcgguaca ccagcaccaa ggaggugcuc     4200

gacgccaccc ugauccacca guccaucacg ggccuguacg agacccgcau cgaccucagc     4260

cagcucgggg gcgacaagcg gcccgccgcc accaagaagg ccgggcaggc gaagaagaag     4320

aaguaaggac uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua     4380

gccugucuuu cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa agaauu                                          4466


<210>  2491
<211>  5249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2491
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaacuuc      180

aagauccugc ccaucgccau cgaccucggc gugaagaaca ccgggguguu cagcgccuuc      240

uaccagaagg gcaccucccu ggagcggcug gacaacaaga acgggaaggu cuacgagcuc      300

agcaaggacu ccuacacgcu gcugaugaac aaccgcaccg cccggcgcca ccagcggcgc      360

ggcaucgacc ggaagcagcu cgugaagcgc cuguucaagc ugaucuggac cgagcagcuc      420

aaccuggagu gggacaagga cacccagcag gccaucagcu uccuguucaa ccggcgcggc      480

uucuccuuca ucacggacgg guacagcccc gaguaccuca acaucgugcc cgagcagguc      540

aaggcgaucc ugauggacau cuucgacgac uacaacggcg aggacgaccu ggacuccuac      600

cucaagcugg ccaccgagca ggagagcaag aucuccgaga ucuacaacaa gcugaugcag      660

aagauccucg aguucaagcu gaugaagcug ugcaccgaca ucaaggacga caaggugagc      720

accaagacgc ucaaggagau caccuccuac gaguucgagc ugcuggccga cuaccucgcc      780

aacuacagcg agucccugaa gacccagaag uucagcuaca ccgacaagca ggggaaccug      840

aaggagcucu ccuacuacca ccacgacaag uacaacaucc aggaguuccu gaagcggcac      900

gcgacgauca acgaccgcau ccuggacacc cuccugaccg acgaccugga caucuggaac      960

uucaacuucg agaaguucga cuucgacaag aacgaggaga agcuccagaa ccaggaggac     1020

aaggaccaca uccaggccca ccuccaccac uucguguucg ccgucaacaa gaucaagagc     1080

gagauggccu ccggcgggcg gcaccgcagc caguacuucc aggagaucac caacgugcug     1140

gacgagaaca accaccagga gggcuaccug aagaacuucu gcgagaaccu gcacaacaag     1200

aaguacucca accugagcgu gaagaaccuc gucaaccuga ucggcaaccu gagcaaccuc     1260

gagcugaagc cgcugcggaa guacuucaac gacaagaucc acgccaaggc ggaccacugg     1320

gacgagcaga aguucacgga gaccuacugc cacuggaucc ucggggagug gcgcgugggc     1380

gugaaggacc aggacaagaa ggacggggcc aaguacuccu acaaggaccu gugcaacgag     1440

cugaagcaga aggucaccaa ggccggccuc guggacuucc ugcuggagcu ggaccccugc     1500

cggaccaucc cgcccuaccu cgacaacaac aaccgcaagc cgcccaagug ccagagccug     1560

auccugaacc ccaaguuccu cgacaaccag uaccccaacu ggcagcagua ccugcaggag     1620

cugaagaagc uccaguccau ccagaacuac cuggacagcu ucgagacgga ccugaaggug     1680

cucaagucca gcaaggacca gcccuacuuc gucgaguaca aguccagcaa ccagcagauc     1740

gccuccggcc agcgggacua caaggaccug gacgcccgca uccugcaguu caucuucgac     1800

cgggugaagg cgagcgacga gcuccugcug aacgagaucu acuuccaggc caagaagcuc     1860

aagcagaagg ccuccagcga gcuggagaag cuggagucca gcaagaagcu cgacgaggug     1920

aucgccaacu cccagcugag ccagauccug aagucccagc acaccaacgg gaucuucgag     1980

cagggcaccu uccuccaccu ggucugcaag uacuacaagc agcgccagcg cgcccgggac     2040

agccgccugu acaucaugcc cgaguaccgg uacgacaaga agcuccacaa guacaacaac     2100

accgggcgcu ucgacgacga caaccagcug cugacguacu gcaaccacaa gccccggcag     2160

aagcgcuacc agcuccugaa cgaccuggcg ggcgugcucc aggugucccc caacuuccug     2220

aaggacaaga ucgggagcga cgacgaccug uucaucagca aguggcucgu cgagcacauc     2280

cggggcuuca agaaggccug cgaggacucc cugaagaucc agaaggacaa ccgcggccug     2340

cucaaccaca agaucaacau cgcccggaac accaagggga agugcgagaa ggagaucuuc     2400

aaccugaucu gcaagaucga gggcagcgag gacaagaagg ggaacuacaa gcacggccug     2460

gccuacgagc ucggggugcu gcuguucggc gagccgaacg aggccuccaa gcccgaguuc     2520

gaccgcaaga ucaagaaguu caacagcauc uacuccuucg cgcagaucca gcagaucgcc     2580

uucgccgagc ggaagggcaa cgccaacacg ugcgcggucu gcagcgccga caacgcccac     2640

cgcaugcagc agaucaagau caccgagccc guggaggaca acaaggacaa gaucauccuc     2700

uccgccaagg cccagcggcu gcccgcgauc ccgacccgca ucguggacgg ggccgucaag     2760

aagauggcca ccauccuggc caagaacauc guggacgaca acuggcagaa caucaagcag     2820

guccucagcg ccaagcacca gcugcacauc cccaucauca ccgaguccaa cgcguucgag     2880

uucgagcccg cccuggccga cgugaagggc aagagccuca aggaccggcg caagaaggcc     2940

cuggagcgga ucucccccga gaacaucuuc aaggacaaga acaaccgcau caaggaguuc     3000

gccaagggga ucagcgcgua cuccggcgcc aaccugaccg acggggacuu cgacggcgcc     3060

aaggaggagc ucgaccacau caucccgcgg agccacaaga aguacggcac gcugaacgac     3120

gaggccaacc ugaucugcgu gacccgcggg gacaacaaga acaagggcaa ccgcaucuuc     3180

ugccuccggg accuggcgga caacuacaag cugaagcagu ucgagaccac cgacgaccuc     3240

gagaucgaga agaagaucgc cgacacgauc ugggacgcca acaagaagga cuucaaguuc     3300

gggaacuacc gcuccuucau caaccugacg ccccaggagc agaaggccuu ccggcacgcc     3360

cuguuccucg cggacgagaa ccccaucaag caggccguga uccgcgccau caacaaccgg     3420

aaccgcaccu ucgucaacgg cacccagcgg uacuucgccg aggugcuggc caacaacauc     3480

uaccugcgcg cgaagaagga gaaccucaac accgacaaga ucagcuucga cuacuucggc     3540

auccccacca ucgggaacgg ccgggggauc gccgagaucc gccagcugua cgagaaggug     3600

gacuccgaca uccaggccua cgccaagggc gacaagccgc aggccagcua cagccaccug     3660

aucgacgcga ugcucgccuu cugcaucgcc gccgacgagc accggaacga cggguccauc     3720

ggccuggaga ucgacaagaa cuacagccug uacccccucg acaagaacac cggcgagguc     3780

uucaccaagg acaucuucuc ccagaucaag aucaccgaca acgaguucag cgacaagaag     3840

cuggugcgca agaaggcgau cgagggguuc aacacgcacc ggcagaugac ccgcgacggc     3900

aucuacgccg agaacuaccu gcccauccuc auccacaagg agcugaacga ggugcggaag     3960

ggguacaccu ggaagaacuc cgaggagauc aagaucuuca agggcaagaa guacgacauc     4020

cagcagcuga acaaccucgu cuacugccug aaguucgugg acaagccgau cagcaucgac     4080

auccagaucu ccacccugga ggagcuccgc aacauccuga cgaccaacaa caucgccgcc     4140

accgccgagu acuacuacau caaccugaag acccagaagc uccacgagua cuacaucgag     4200

aacuacaaca cggcgcuggg guacaagaag uacagcaagg agauggaguu ccugcggucc     4260

cucgccuacc gcagcgagcg ggugaagauc aaguccaucg acgacgucaa gcagguccug     4320

gacaaggaca gcaacuucau caucggcaag aucacccugc ccuucaagaa ggaguggcag     4380

cgccucuacc gggaguggca gaacaccacc aucaaggacg acuacgaguu ccugaagucc     4440

uucuucaacg ucaagagcau cacgaagcug cacaagaagg ugcgcaagga cuucucccuc     4500

cccaucagca ccaacgaggg caaguuccug gugaagcgca agaccuggga caacaacuuc     4560

aucuaccaga uccugaacga cuccgacagc cgggccgacg ggaccaagcc cuucaucccg     4620

gccuucgaca ucuccaagaa cgagaucgug gaggccauca ucgacagcuu cacgagcaag     4680

aacaucuucu ggcuccccaa gaacaucgag cugcagaagg uggacaacaa gaacaucuuc     4740

gcgaucgaca ccuccaagug guucgaggug gagaccccca gcgaccugcg cgacaucggc     4800

aucgccacca uccaguacaa gaucgacaac aacucccggc ccaaggugcg cgucaagcug     4860

gacuacguga ucgacgacga cagcaagauc aacuacuuca ugaaccacuc ccuccucaag     4920

agccgguacc cggacaaggu gcuggagauc cugaagcagu ccacgaucau cgaguucgag     4980

agcuccgggu ucaacaagac caucaaggag augcugggca ugaagcuggc cgggaucuac     5040

aacgagacca gcaacaacaa gcgccccgcc gccaccaaga aggcgggcca ggccaagaag     5100

aagaaguaag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     5160

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5220

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       5249


<210>  2492
<211>  3521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2492
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaagcgg      180

aacuacaucc ugggccucga caucgggauc accagcgugg gcuacgggau caucgacuac      240

gagacccgcg acgugaucga cgccggcguc cggcuguuca aggaggccaa cguggagaac      300

aacgagggcc gccgguccaa gcgcggggcc cggcgccuga agcggcgccg gcgccaccgg      360

auccagcgcg ugaagaagcu ccuguucgac uacaaccugc ucacggacca cagcgagcug      420

uccggcauca accccuacga ggcccggguc aaggggcuga gccagaagcu cuccgaggag      480

gaguucagcg cggcccugcu gcaccucgcc aagcgccggg gcgugcacaa cgugaacgag      540

gucgaggagg acaccgggaa cgagcugucc accaaggagc agaucagccg caacuccaag      600

gcccuggagg agaaguacgu ggcggagcuc cagcuggagc ggcugaagaa ggacggcgag      660

gugcgcggca gcaucaaccg guucaagacc uccgacuacg ucaaggaggc caagcagcuc      720

cugaaggugc agaaggccua ccaccagcug gaccagagcu ucaucgacac guacaucgac      780

cuccuggaga cccgccgcac cuacuacgag gggcccggcg aggggucccc cuucggcugg      840

aaggacauca aggaguggua cgagaugcug augggccacu gcaccuacuu cccggaggag      900

cuccggagcg ugaaguacgc cuacaacgcc gaccuguaca acgcgcugaa cgaccucaac      960

aaccugguca ucacgcgcga cgagaacgag aagcuggagu acuacgagaa guuccagauc     1020

aucgagaacg uguucaagca gaagaagaag cccacccuca agcagaucgc caaggagauc     1080

cuggugaacg aggaggacau caagggguac cgggucaccu ccaccggcaa gcccgaguuc     1140

acgaaccuga agguguacca cgacaucaag gacaucaccg cccgcaagga gaucaucgag     1200

aacgccgagc uccuggacca gaucgccaag auccugacca ucuaccagag cuccgaggac     1260

auccaggagg agcucaccaa ccugaacagc gagcugacgc aggaggagau cgagcagauc     1320

uccaaccuca agggguacac cggcacccac aaccugagcc ugaaggcgau caaccucauc     1380

cuggacgagc uguggcacac caacgacaac cagaucgcca ucuucaaccg gcucaagcug     1440

gugcccaaga aggucgaccu gagccagcag aaggagaucc cgacgacccu cguggacgac     1500

uucauccugu cccccguggu caagcgcagc uucauccagu ccaucaaggu gaucaacgcc     1560

aucaucaaga aguacgggcu gcccaacgac aucaucaucg agcuggcccg ggagaagaac     1620

agcaaggacg cccagaagau gaucaacgag augcagaagc gcaaccggca gaccaacgag     1680

cgcaucgagg agaucauccg gaccacgggc aaggagaacg cgaaguaccu caucgagaag     1740

aucaagcugc acgacaugca ggagggcaag ugccuguacu cccucgaggc caucccccug     1800

gaggaccugc ucaacaaccc guucaacuac gaggucgacc acaucauccc ccgcagcgug     1860

uccuucgaca acagcuucaa caacaaggug cuggucaagc aggaggagaa cuccaagaag     1920

gggaaccgga cccccuucca guaccugagc uccagcgacu ccaagaucag cuacgagacc     1980

uucaagaagc acauccucaa ccuggccaag ggcaaggggc gcaucuccaa gaccaagaag     2040

gaguaccugc ucgaggagcg ggacaucaac cgcuucagcg ugcagaagga cuucaucaac     2100

cggaaccugg uggacacgcg cuacgccacc cggggccuga ugaaccuccu gcgcuccuac     2160

uuccgcguca acaaccugga cgugaagguc aagagcauca acgggggcuu caccuccuuc     2220

cuccggcgca aguggaaguu caagaaggag cggaacaagg gcuacaagca ccacgccgag     2280

gacgcgcuga ucaucgccaa cgccgacuuc aucuucaagg aguggaagaa gcuggacaag     2340

gccaagaagg ugauggagaa ccagauguuc gaggagaagc aggcggagag caugcccgag     2400

aucgagaccg agcaggagua caaggagauc uucaucacgc cgcaccagau caagcacauc     2460

aaggacuuca aggacuacaa guacagccac cgcguggaca agaagcccaa ccgggagcuc     2520

aucaacgaca cccuguacuc cacccgcaag gacgacaagg ggaacacccu gaucgugaac     2580

aaccucaacg gccuguacga caaggacaac gacaagcuga agaagcucau caacaagagc     2640

cccgagaagc ugcugaugua ccaccacgac ccccagacgu accagaagcu caagcugauc     2700

auggagcagu acggggacga gaagaacccg cuguacaagu acuacgagga gaccggcaac     2760

uaccucacca aguacuccaa gaaggacaac gggcccguca ucaagaagau caaguacuac     2820

ggcaacaagc ugaacgccca ccuggacauc accgacgacu accccaacag ccggaacaag     2880

guggugaagc ucucccugaa gcccuaccgc uucgacgucu accuggacaa cggcguguac     2940

aaguucguga ccgucaagaa ccucgacgug aucaagaagg agaacuacua cgaggugaac     3000

agcaagugcu acgaggaggc caagaagcug aagaagaucu ccaaccaggc cgaguucauc     3060

gccagcuucu acaacaacga ccugaucaag aucaacgggg agcucuaccg ggucaucggc     3120

gugaacaacg accugcugaa ccgcaucgag gugaacauga ucgacaucac guaccgggag     3180

uaccucgaga acaugaacga caagcgcccg ccccggauca ucaagaccau cgcguccaag     3240

acccagagca ucaagaagua cuccaccgac auccugggga accuguacga ggucaagagc     3300

aagaagcacc cccagaucau caagaagggc aagcgccccg ccgccacgaa gaaggccggc     3360

caggccaaga agaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3420

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3521


<210>  2493
<211>  4589
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2493
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgcuguuc      180

aacaagugca ucaucaucag caucaaccuc gacuucucca acaaggagaa gugcaugacc      240

aagcccuaca gcaucggccu ggacaucggg accaacuccg ugggcugggc cgugaucacg      300

gacaacuaca agguccccag caagaagaug aaggugcugg ggaacaccuc caagaaguac      360

aucaagaaga accuccuggg cgugcugcug uucgacagcg gcaucaccgc cgaggggcgg      420

cgccugaagc ggaccgcccg ccggcgcuac acgcggcgcc ggaaccgcau ccuguaccuc      480

caggagaucu ucuccaccga gauggccacc cuggacgacg cguucuucca gcggcuggac      540

gacagcuucc ucguccccga cgacaagcgc gacuccaagu acccgaucuu cggcaaccug      600

guggaggaga agguguacca cgacgaguuc cccaccaucu accaccuccg gaaguaccuc      660

gccgacagca cgaagaaggc cgaccugcgc cuggucuacc ucgcccuggc gcacaugauc      720

aaguaccggg ggcacuuccu gaucgagggc gaguucaacu ccaagaacaa cgacauccag      780

aagaacuucc aggacuuccu cgacaccuac aacgccaucu ucgagagcga ccugucccug      840

gagaacagca agcagcucga ggagaucgug aaggacaaga ucuccaagcu ggagaagaag      900

gaccgcaucc ugaagcuguu ccccggggag aagaacagcg gcaucuucuc cgaguuccug      960

aagcugaucg ugggcaacca ggccgacuuc cggaagugcu ucaaccucga cgagaaggcc     1020

agccugcacu ucuccaagga gagcuacgac gaggaccugg agacccuccu gggguacauc     1080

ggcgacgacu acagcgacgu cuuccugaag gccaagaagc ucuacgacgc gauccugcug     1140

uccggguucc ucaccgugac ggacaacgag accgaggcgc cccugagcuc cgccaugauc     1200

aagcgcuaca acgagcacaa ggaggaccug gcccuccuga aggaguacau ccggaacauc     1260

agccugaaga ccuacaacga gguguucaag gacgacacca agaacggcua cgccggcuac     1320

aucgacggga agacgaacca ggaggacuuc uacgucuacc ucaagaaccu gcuggccgag     1380

uucgagggcg ccgacuacuu ccucgagaag aucgaccgcg aggacuuccu gcggaagcag     1440

cgcaccuucg acaacggguc caucccguac cagauccacc uccaggagau gcgcgccauc     1500

cuggacaagc aggccaaguu cuaccccuuc cucgccaaga acaaggagcg gaucgagaag     1560

auccugaccu uccgcauccc cuacuacgug ggcccccugg cgcgggggaa cagcgacuuc     1620

gccuggucca uccgcaagcg gaacgagaag aucaccccgu ggaacuucga ggacgugauc     1680

gacaaggaga gcuccgccga ggccuucauc aaccgcauga cgagcuucga ccucuaccug     1740

cccgaggaga agguccugcc caagcacucc cuccuguacg agaccuucaa cguguacaac     1800

gagcugacca aggugcgguu caucgccgag agcaugcgcg acuaccaguu ccucgacucc     1860

aagcagaaga aggacaucgu ccggcuguac uucaaggaca agcgcaaggu gaccgacaag     1920

gacaucaucg aguaccugca cgcgaucuac ggcuacgacg gcaucgagcu caaggggauc     1980

gagaagcagu ucaacagcuc ccugagcacg uaccacgacc ugcucaacau caucaacgac     2040

aaggaguucc uggacgacuc cagcaacgag gccaucaucg aggagaucau ccacacccug     2100

accaucuucg aggaccggga gaugaucaag cagcgccucu ccaaguucga gaacaucuuc     2160

gacaagagcg ugcugaagaa gcugagccgg cgccacuaca ccggcugggg gaagcucucc     2220

gccaagcuga ucaacggcau ccgggacgag aaguccggga acacgauccu ggacuaccuc     2280

aucgacgacg gcaucagcaa ccgcaacuuc augcagcuga uccacgacga cgcccugagc     2340

uucaagaaga agauccagaa ggcgcagauc aucggcgacg aggacaaggg gaacaucaag     2400

gaggucguga agucccuccc cggcagcccg gccaucaaga aggggauccu gcaguccauc     2460

aagaucgucg acgagcuggu gaaggugaug ggcgggcgga agcccgagag caucgucgug     2520

gagauggccc gcgagaacca guacaccaac cagggcaagu ccaacagcca gcagcggcuc     2580

aagcgccugg agaagucccu gaaggagcuc ggcagcaaga uccugaagga gaacaucccc     2640

gccaagcugu ccaagaucga caacaacgcc cuccagaacg accgccugua ccuguacuac     2700

cuccagaacg ggaaggacau guacaccggc gacgaccugg acaucgaccg gcugagcaac     2760

uacgacaucg accacaucau cccccaggcg uuccucaagg acaacuccau cgacaacaag     2820

gugcugguca gcuccgccag caaccgcggg aaguccgacg acuucccgag ccuggaggug     2880

gugaagaagc ggaagaccuu cugguaccag cuccucaaga gcaagcugau cucccagcgc     2940

aaguucgaca accugacgaa ggccgagcgg ggcggccugc ugcccgagga caaggccggg     3000

uucauccagc gccagcucgu cgagacccgg cagaucacca agcacguggc ccgccugcug     3060

gacgagaagu ucaacaacaa gaaggacgag aacaaccggg cggugcgcac cgucaagauc     3120

aucacgcuca agagcacccu ggugucccag uuccggaagg acuucgagcu guacaaggug     3180

cgcgagauca acgacuucca ccacgcccac gacgccuacc ucaacgccgu caucgccagc     3240

gcgcugcuga agaaguaccc caagcucgag cccgaguucg uguacggcga cuacccgaag     3300

uacaacuccu uccgggagcg caagagcgcc accgagaagg uguacuucua cuccaacauc     3360

augaacaucu ucaagaaguc caucagccug gccgacgggc gggucaucga gcgcccccug     3420

aucgagguga acgaggagac cggcgagagc guguggaaca aggaguccga ccucgccacc     3480

guccggcgcg ugcugagcua cccccaggug aacgucguga agaaggugga ggagcagaac     3540

cacgggcugg accggggcaa gcccaagggc cucuucaacg cgaaccuguc cagcaagccg     3600

aagcccaacu ccaacgagaa ccuggucggg gccaaggagu accucgaccc caagaaguac     3660

ggcggguacg ccggcaucag caacuccuuc gccgugcugg ugaaggggac gaucgagaag     3720

ggcgccaaga agaagaucac caacguccug gaguuccagg gcaucagcau ccucgaccgc     3780

aucaacuacc ggaaggacaa gcugaacuuc cugcucgaga agggguacaa ggacaucgag     3840

cugaucaucg agcugccgaa guacagccuc uucgagcugu ccgacggcag ccgccgcaug     3900

cuggcgucca uccucagcac caacaacaag cgcggggaga uccacaaggg caaccagauc     3960

uuccuguccc agaaguucgu gaagcugcuc uaccacgcca agcggaucag caacaccauc     4020

aacgagaacc accggaagua cguggagaac cacaagaagg aguucgagga gcuguucuac     4080

uacauccugg aguucaacga gaacuacguc ggggccaaga agaacggcaa gcuccugaac     4140

uccgccuucc agagcuggca gaaccacucc aucgacgagc ugugcagcuc cuucaucggc     4200

cccacgggga gcgagcgcaa gggccucuuc gagcugaccu cccgggggag cgccgcggac     4260

uucgaguucc ugggcgugaa gaucccccgc uaccgggacu acacccccuc cagccuccug     4320

aaggacgcca cccugaucca ccaguccgug acggggcugu acgagacccg caucgaccuc     4380

gccaagcugg gcgagggcaa gcggccggcc gccaccaaga aggcggggca ggccaagaag     4440

aagaaguaag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     4500

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       4589


<210>  2494
<211>  3665
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2494
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgugguac      180

gccagccuga uguccgccca ccaccuccgg gugggcaucg acguggggac ccacagcguc      240

ggccuggcca cccugcgcgu ggacgaccac gggacgccca ucgagcuccu guccgcccug      300

agccacaucc acgacuccgg cgugggcaag gaggggaaga aggaccacga cacccggaag      360

aagcucagcg gcaucgcgcg ccgggcccgc cggcugcugc accaccgccg gacccagcuc      420

cagcagcugg acgagguccu gcgcgaccuc ggguucccca uccccacccc gggcgaguuc      480

cuggaccuga acgagcagac ggaccccuac cggguguggc gcgugcgggc ccgccucguc      540

gaggagaagc ugcccgagga gcugcggggg cccgccaucu ccauggcggu gcgccacauc      600

gcccggcacc gcggcuggcg gaacccguac agcaaggugg agucccuccu gagccccgcc      660

gaggaguccc ccuucaugaa ggcccugcgc gagcggaucc ucgccaccac cggcgagguc      720

cuggacgacg ggaucacccc cggccaggcg auggcccagg uggcccugac gcacaacauc      780

agcaugcgcg ggccggaggg cauccucggc aagcugcacc aguccgacaa cgccaacgag      840

auccgcaaga ucugcgcccg gcagggggug agccccgacg ucugcaagca gcugcuccgc      900

gcgguguuca aggccgacag cccccggggc uccgccgugu cccgcgucgc ccccgacccg      960

cugcccgggc agggcagcuu ccggcgcgcc cccaagugcg accccgaguu ccagcgguuc     1020

cgcaucaucu ccaucguggc gaaccugcgg aucagcgaga ccaaggggga gaaccgcccg     1080

cucaccgccg acgagcggcg ccacgugguc accuuccuga cggaggacag ccaggccgac     1140

cugaccuggg uggacguggc cgagaagcuc ggcguccacc ggcgcgaccu gcggggcacc     1200

gccgugcaca ccgacgacgg ggagcgcucc gcggcccggc cgcccaucga cgccacggac     1260

cgcaucaugc ggcagaccaa gaucagcucc cugaagaccu ggugggagga ggccgacagc     1320

gagcagcgcg gcgcgaugau ccgcuaccuc uacgaggacc ccaccgacuc cgagugcgcc     1380

gagaucaucg ccgagcugcc cgaggaggac caggccaagc uggacagccu ccaccucccc     1440

gccgggcggg cggccuacuc ccgcgagagc cugacggccc uguccgacca caugcuggcc     1500

accaccgacg accugcacga ggcccggaag cgccucuucg gcgucgacga cagcugggcg     1560

ccgcccgccg aggccaucaa cgcccccgug gggaaccccu ccguggaccg gacccugaag     1620

aucgucggcc gcuaccugag cgccguggag uccauguggg gcacgcccga ggugauccac     1680

gucgagcacg ugcgggacgg guucaccagc gagcgcaugg cggacgagcg ggacaaggcc     1740

aaccgccggc gcuacaacga caaccaggag gccaugaaga agauccagcg ggacuacggc     1800

aaggaggggu acaucucccg cggcgacauc gugcggcucg acgcccugga gcugcagggg     1860

ugcgcgugcc uguacugcgg caccaccauc ggcuaccaca cgugccagcu cgaccacauc     1920

gucccgcagg ccgggcccgg cagcaacaac cgccggggga accugguggc cgugugcgag     1980

cgcugcaacc gguccaagag caacaccccc uucgccgucu gggcccagaa gugcggcauc     2040

ccccacgugg gcgugaagga ggcgaucggg cgcguccggg gcuggcgcaa gcagaccccg     2100

aacaccucca gcgaggaccu gacgcgccuc aagaaggagg ugaucgcccg gcugcgccgg     2160

acccaggagg accccgagau cgacgagcgc agcauggagu ccguggccug gauggccaac     2220

gagcugcacc accggaucgc cgcggccuac cccgagacca ccgucauggu guaccgcggg     2280

agcaucacgg ccgccgcccg gaaggcggcc ggcaucgacu cccgcaucaa ccucaucggg     2340

gagaagggcc ggaaggaccg caucgaccgg cgccaccacg ccguggacgc cagcgucgug     2400

gcgcugaugg aggccuccgu ggccaagacc cuggccgagc ggagcucccu ccgcggcgag     2460

cagcggcuga ccgggaagga gcagaccugg aagcaguaca ccggcagcac ggucggggcc     2520

cgcgagcacu ucgagaugug gcggggccac augcugcacc ucaccgagcu guucaacgag     2580

cgccuggcgg aggacaaggu guacgugacc cagaacaucc ggcuccgccu guccgacggg     2640

aacgcccaca ccgucaaccc cagcaagcug gugucccacc gccucggcga cggccugacg     2700

gugcagcaga ucgaccgggc cugcaccccg gcccuguggu gcgcccucac ccgcgagaag     2760

gacuucgacg agaagaacgg gcugcccgcg cgggaggacc gcgccauccg gguccacggc     2820

cacgagauca agagcuccga cuacauccag guguucagca agcgcaagaa gaccgacucc     2880

gaccgggacg agacgcccuu cggggccauc gccgugcgcg gcggguucgu cgagaucggc     2940

cccagcaucc accacgcccg gaucuaccgc guggagggca agaagccggu guacgcgaug     3000

cugcgggucu ucacccacga ccuccugucc cagcgccacg gggaccuguu cagcgccgug     3060

aucccgcccc agagcaucuc caugcggugc gccgagccca agcuccgcaa ggccaucacc     3120

accggcaacg ccacguaccu ggggugggug gucgugggcg acgagcugga gaucaacgug     3180

gacagcuuca ccaaguacgc gaucgggcgg uuccucgagg acuuccccaa caccacccgc     3240

uggcggaucu gcggcuacga cacgaacucc aagcugaccc ugaagcccau cguccucgcc     3300

gccgagggcc uggagaaccc cagcuccgcc gugaacgaga ucguggagcu gaaggggugg     3360

cgcgucgcga ucaacgugcu caccaaggug caccccaccg ucgugcggcg cgacgcccug     3420

ggccggccgc gcuacagcuc ccgcagcaac cugcccacgu ccuggaccau cgagaagcgg     3480

cccgccgcca ccaagaaggc cgggcaggcg aagaagaaga aguaaggacu agucccuguu     3540

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

gaauu                                                                 3665


<210>  2495
<211>  4526
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2495
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaccaag      180

cccuacagca ucggccugga caucgggacc aacuccgugg gcugggccgu gacgaccgac      240

aacuacaagg uccccagcaa gaagaugaag gugcucggga acaccuccaa gaaguacauc      300

aagaagaacc ugcugggcgu gcuccuguuc gacagcggca ucaccgccga ggggcggcgc      360

cugaagcgga cggcccgccg gcgcuacacc cggcgccgga accgcauccu cuaccugcag      420

gagaucuucu ccaccgagau ggccacccug gacgacgcgu ucuuccagcg gcucgacgac      480

agcuuccugg uccccgacga caagcgcgac uccaaguacc cgaucuucgg caaccuggug      540

gaggagaagg ccuaccacga cgaguucccc acgaucuacc accuccggaa guaccuggcc      600

gacagcacca agaaggccga ccugcgccuc guguaccugg cgcuggccca caugaucaag      660

uaccgggggc acuuccucau cgagggcgag uucaacucca agaacaacga cauccagaag      720

aacuuccagg acuuccugga caccuacaac gccaucuucg agagcgaccu gucccucgag      780

aacagcaagc agcuggagga gaucgucaag gacaagaucu ccaagcugga gaagaaggac      840

cgcauccuca agcuguuccc cggggagaag aacagcggca ucuucuccga guuccugaag      900

cucaucgugg gcaaccaggc cgacuuccgg aagugcuuca accuggacga gaaggccagc      960

cugcacuucu ccaaggagag cuacgacgag gaccucgaga cccugcuggg guacaucggc     1020

gacgacuacu ccgacguguu ccucaaggcg aagaagcugu acgacgccau ccugcucagc     1080

ggguuccuga cggucaccga caacgagacc gaggcgcccc ugagcuccgc caugaucaag     1140

cgcuacaacg agcacaagga ggaccucgcc cugcugaagg aguacauccg gaacaucagc     1200

cucaagaccu acaacgaggu guucaaggac gacacgaaga acggcuacgc cggcuacauc     1260

gacgggaaga ccaaccagga ggacuucuac guguaccuga agaagcugcu cgccgaguuc     1320

gagggcgccg acuacuuccu ggagaagauc gaccgcgagg acuuccugcg gaagcagcgc     1380

accuucgaca acggguccau cccguaccag auccaccucc aggagaugcg cgccauccug     1440

gacaagcagg ccaaguucua ccccuuccug gcgaagaaca aggagcggau cgagaagauc     1500

cugaccuucc gcauccccua cuacgucggc ccccucgccc gggggaacag cgacuucgcc     1560

ugguccaucc gcaagcggaa cgagaagauc acgccgugga acuucgagga cgugaucgac     1620

aaggagagcu ccgccgaggc cuucaucaac cgcaugacca gcuucgaccu guaccugccc     1680

gaggagaagg ugcuccccaa gcacucccug cuguacgaga ccuucaacgu cuacaacgag     1740

cucaccaagg ugcgguucau cgcggagagc augcgcgacu accaguuccu ggacuccaag     1800

cagaagaagg acaucgugcg gcuguacuuc aaggacaagc gcaaggucac ggacaaggac     1860

aucaucgagu accuccacgc caucuacggc uacgacggca ucgagcugaa ggggaucgag     1920

aagcaguuca acagcucccu gagcaccuac cacgaccucc ugaacaucau caacgacaag     1980

gaguuccugg acgacuccag caacgaggcc aucaucgagg agaucaucca cacccucacc     2040

aucuucgagg accgggagau gaucaagcag cgccugucca aguucgagaa caucuucgac     2100

aagagcgugc ugaagaagcu cucccggcgc cacuacacgg gcugggggaa gcugagcgcc     2160

aagcugauca acggcauccg ggacgagaag uccgggaaca ccauccucga cuaccugauc     2220

gacgacggca ucagcaaccg caacuucaug cagcugaucc acgacgacgc gcucagcuuc     2280

aagaagaaga uccagaaggc ccagaucauc ggcgacgagg acaaggggaa caucaaggag     2340

guggucaagu cccugcccgg cagcccggcc aucaagaagg ggauccugca guccaucaag     2400

aucguggacg agcucgucaa ggugaugggc gggcggaagc ccgagagcau cguggucgag     2460

auggcccgcg agaaccagua caccaaccag ggcaagucca acagccagca gcggcugaag     2520

cgccuggaga agucccucaa ggagcugggc agcaagaucc ugaaggagaa cauccccgcc     2580

aagcucucca agaucgacaa caacgcgcug cagaacgacc gccuguaccu cuacuaccug     2640

cagaacggga aggacaugua caccggcgac gaccuggaca ucgaccggcu cagcaacuac     2700

gacaucgacc acaucauccc ccaggccuuc cugaaggaca acuccaucga caacaaggug     2760

cuggugagcu ccgccagcaa ccgcgggaag uccgacgacg ucccgagccu cgagguggug     2820

aagaagcgga agacguucug guaccagcug cucaagagca agcugaucuc ccagcgcaag     2880

uucgacaacc ugaccaaggc cgagcggggc ggccugagcc ccgaggacaa ggccggguuc     2940

auccagcgcc agcucgucga gacccggcag aucaccaagc acguggcgcg ccugcuggac     3000

gagaaguuca acaacaagaa ggacgagaac aaccgggccg ugcgcacggu caagaucauc     3060

acccucaagu ccacccuggu gagccaguuc cggaaggacu ucgagcugua caaggugcgc     3120

gagaucaacg acuuccacca cgcccacgac gccuaccuca acgccgucgu ggcguccgcc     3180

cugcugaaga aguaccccaa gcucgagccc gaguucgugu acggcgacua cccgaaguac     3240

aacagcuucc gggagcgcaa guccgccacc gagaaggucu acuucuacag caacaucaug     3300

aacaucuuca agaaguccau cagccuggcc gacgggcggg ugaucgagcg cccccugauc     3360

gaggugaacg aggagaccgg cgaguccguc uggaacaagg agagcgaccu cgcgacggug     3420

cggcgcgugc uguccuaccc ccaggucaac guggugaaga aggucgagga gcagaaccac     3480

gggcuggacc ggggcaagcc caagggccuc uucaacgcca accugagcuc caagccgaag     3540

cccaacagca acgagaaccu ggugggggcc aaggaguacc ucgaccccaa gaaguacggc     3600

ggguacgccg gcaucuccaa cagcuucacc gugcugguca aggggaccau cgagaagggc     3660

gccaagaaga agaucaccaa cgugcuggag uuccagggca ucuccauccu cgaccgcauc     3720

aacuaccgga aggacaagcu gaacuuccug cucgagaagg gguacaagga caucgagcug     3780

aucaucgagc ugccgaagua cagccucuuc gagcugagcg acggcucccg ccgcaugcug     3840

gcgagcaucc ucuccacgaa caacaagcgc ggggagaucc acaagggcaa ccagaucuuc     3900

cugagccaga aguucgugaa gcugcucuac cacgccaagc ggaucuccaa caccaucaac     3960

gagaaccacc ggaaguacgu cgagaaccac aagaaggagu ucgaggagcu guucuacuac     4020

auccuggagu ucaacgagaa cuacgugggg gccaagaaga acggcaagcu ccugaacagc     4080

gccuuccagu ccuggcagaa ccacagcauc gacgagcugu gcuccagcuu caucggcccc     4140

accggguccg agcgcaaggg ccucuucgag cugaccagcc ggggguccgc cgcggacuuc     4200

gaguuccugg gcgugaagau cccccgcuac cgggacuaca cgcccagcuc ccuccugaag     4260

gacgccaccc ugauccacca gagcgucacc gggcucuacg agacccgcau cgaccuggcc     4320

aagcugggcg agggcaagcg gccggccgcc acgaagaagg cggggcaggc caagaagaag     4380

aaguaaggac uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua     4440

gccugucuuu cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa agaauu                                          4526


<210>  2496
<211>  3608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2496
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcggccgcc      180

uucaagccca acagcaucaa cuacauccug ggccucgaca ucgggaucgc cuccgugggc      240

ugggccaugg uggagaucga cgaggaggag aaccccaucc ggcugaucga ccuggggguc      300

cgcguguucg agcgggcgga ggugcccaag accggcgaca gccucgccau ggcccgccgg      360

cuggcccgcu ccguccggcg ccugacccgg cgccgggcgc accgccuccu gcggacgcgc      420

cggcugcuca agcgcgaggg cgugcugcag gccgccaacu ucgacgagaa cgggcugauc      480

aagagccucc cgaacacccc cuggcagcug cgggccgccg cgcuggaccg caagcucacg      540

ccccuggagu gguccgccgu gcugcuccac cugaucaagc accggggcua ccugagccag      600

cgcaagaacg agggggagac cgccgacaag gagcucggcg cccugcugaa gggggucgcc      660

ggcaacgcgc acgcccucca gaccggcgac uuccggaccc ccgccgagcu ggcccugaac      720

aaguucgaga aggaguccgg gcacauccgc aaccagcgca gcgacuacuc ccacaccuuc      780

agccggaagg accuccaggc cgagcugauc cuccuguucg agaagcagaa ggaguucggc      840

aacccgcacg uguccggggg ccugaaggag ggcaucgaga cccugcucau gacgcagcgc      900

cccgcgcuga gcggggacgc cgugcagaag augcugggcc acugcaccuu cgagcccgcc      960

gagcccaagg ccgccaagaa caccuacacc gcggagcggu ucaucuggcu cacgaagcug     1020

aacaaccugc gcauccucga gcaggggucc gagcggccgc ugaccgacac cgagcgcgcc     1080

acccugaugg acgagcccua ccgcaagagc aagcucacgu acgcccaggc ccggaagcug     1140

cugggccucg aggacaccgc guucuucaag gggcugcggu acggcaagga caacgccgag     1200

gccuccaccc ugauggagau gaaggccuac cacgccauca gccgcgcgcu cgagaaggag     1260

ggccugaagg acaagaaguc cccgcugaac cugagccccg agcuccagga cgagaucggg     1320

accgccuucu cccuguucaa gacggacgag gacaucaccg gccggcugaa ggaccgcauc     1380

cagccggaga uccucgaggc ccugcugaag cacaucagcu ucgacaaguu cguccagauc     1440

agccucaagg cccugcggcg caucgugccc cugauggagc aggggaagcg guacgacgag     1500

gccugcgcgg agaucuacgg cgaccacuac gggaagaaga acaccgagga gaagaucuac     1560

cucccgccca uccccgccga cgagauccgc aaccccgugg uccugcgggc ccugucccag     1620

gcccgcaagg ugaucaacgg cgugguccgg cgcuacggca gccccgcccg cauccacauc     1680

gagaccgcgc gggagguggg gaaguccuuc aaggaccgca aggagaucga gaagcggcag     1740

gaggagaacc gcaaggaccg ggagaaggcc gccgccaagu uccgcgagua cuuccccaac     1800

uucgugggcg agcccaagag caaggacauc cucaagcugc ggcuguacga gcagcagcac     1860

gggaagugcc ucuacuccgg caaggagauc aaccuggggc gccugaacga gaagggcuac     1920

gucgagaucg accacgcgcu ccccuucagc cggacguggg acgacuccuu caacaacaag     1980

gugcuggugc ugggcagcga gaaccagaac aaggggaacc agacccccua cgaguacuuc     2040

aacggcaagg acaacucccg cgaguggcag gaguucaagg cccgggucga gaccagccgc     2100

uucccccggu ccaagaagca gcgcauccuc cugcagaagu ucgacgagga cggguucaag     2160

gagcggaacc ugaacgacac ccgcuacgug aaccgguucc ucugccaguu cgucgccgac     2220

cgcaugcggc ugacgggcaa gggcaagaag cgcguguucg ccagcaacgg gcagaucacc     2280

aaccugcucc ggggcuucug ggggcugcgc aaggugcgcg ccgagaacga ccggcaccac     2340

gcgcuggacg ccgucguggu ggccugcucc accgucgcca ugcagcagaa gaucacccgc     2400

uucgugcggu acaaggagau gaacgccuuc gacggcaaga cgaucgacaa ggagaccggg     2460

gaggugcucc accagaagac ccacuucccg cagcccuggg aguucuucgc gcaggagguc     2520

augauccgcg uguucggcaa gcccgacggc aagccggagu ucgaggaggc cgacacccug     2580

gagaagcugc ggacgcuccu ggccgagaag cugagcuccc gccccgaggc cgugcacgag     2640

uacgucacgc cccucuucgu gagccgggcc cccaaccgca agauguccgg gcagggccac     2700

auggagaccg ucaagagcgc gaagcggcug gacgaggggg uguccgugcu gcgcgugccg     2760

cucacccagc ugaagcugaa ggaccucgag aagaugguca accgggagcg cgagcccaag     2820

cuguacgagg cccugaaggc ccggcucgag gcccacaagg acgaccccgc gaaggccuuc     2880

gccgagcccu ucuacaagua cgacaaggcc ggcaaccgca cccagcaggu caaggccgug     2940

cggguggagc agguccagaa gaccggggug ugggugcgca accacaacgg caucgcggac     3000

aacgccacca uggugcgggu ggacgucuuc gagaagggcg acaaguacua ccuggugccg     3060

aucuacagcu ggcaggucgc caaggggauc cugcccgacc gcgccguggu gcagggcaag     3120

gacgaggagg acuggcagcu caucgacgac agcuucaacu ucaaguucuc ccugcacccc     3180

aacgaccugg uggaggucau caccaagaag gcccggaugu ucggguacuu cgcgagcugc     3240

caccgcggca ccgggaacau caacauccgc auccacgacc ucgaccacaa gaucggcaag     3300

aacggcaucc uggaggggau cggcgugaag acggcccugu ccuuccagaa guaccagauc     3360

gacgagcucg ggaaggagau ccggcccugc cgccugaaga agcggccgcc cgugcgcaag     3420

cggcccgccg ccaccaagaa ggccggccag gcgaagaaga agaaguaagg acuagucccu     3480

guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaagaauu                                                              3608


<210>  2497
<211>  4364
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2497
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaagaag      180

cccuacacca ucggccugga caucgggacc aacagcgugg gcugggccgu gcucacggac      240

caguacgacc uggucaagcg gaagaugaag aucgccgggg acuccgagaa gaagcagauc      300

aagaagaacu ucuggggcgu gcgccuguuc gacgagggcc agaccgccgc cgaccggcgc      360

auggcgcgga ccgcccgccg gcgcaucgag cggcgccgga accgcaucag cuaccuccag      420

gggaucuucg ccgaggagau guccaagacc gacgccaacu ucuucugccg gcugagcgac      480

uccuucuacg uggacaacga gaagcgcaac agccggcacc ccuucuucgc gacgaucgag      540

gaggaggucg aguaccacaa gaacuacccc accaucuacc accuccgcga ggagcuggug      600

aacuccagcg agaaggccga ccugcggcug guguaccucg cccuggccca caucaucaag      660

uaccgcggca acuuccugau cgagggggcc cucgacaccc agaacaccuc cgucgacggc      720

aucuacaagc aguucaucca gacguacaac cagguguucg cgagcgggau cgaggacggc      780

ucccugaaga agcuggagga caacaaggac guggccaaga uccucgucga gaaggugacc      840

cggaaggaga agcuggagcg cauccugaag cucuacccgg gcgagaaguc cgccgggaug      900

uucgcccagu ucaucagccu gaucgugggc agcaagggga acuuccagaa gcccuucgac      960

cugaucgaga aguccgacau cgagugcgcc aaggacagcu acgaggagga ccucgagucc     1020

cugcuggcgc ucaucggcga cgaguacgcc gagcuguucg ucgccgccaa gaacgccuac     1080

agcgcggugg ugcuguccag caucaucacc gucgccgaga ccgagacgaa cgccaagcuc     1140

agcgccucca ugaucgagcg guucgacacc cacgaggagg accugggcga gcugaaggcc     1200

uucaucaagc uccaccuccc caagcacuac gaggagaucu ucagcaacac cgagaagcac     1260

ggguacgcgg gcuacaucga cgggaagacc aagcaggccg acuucuacaa guacaugaag     1320

augacgcugg agaacaucga gggcgccgac uacuucaucg ccaagaucga gaaggagaac     1380

uuccugcgca agcagcgcac cuucgacaac ggggcgaucc cccaccagcu gcaccuggag     1440

gagcucgagg ccauccugca ccagcaggcc aaguacuacc cguuccugaa ggagaacuac     1500

gacaagauca agucccucgu gaccuuccgg auccccuacu ucgugggccc ccuggccaac     1560

ggccagagcg aguucgccug gcugacccgc aaggcggacg gggagauccg gcccuggaac     1620

aucgaggaga aggucgacuu cggcaagucc gccguggacu ucaucgagaa gaugacgaac     1680

aaggacaccu accucccgaa ggagaacgug cugcccaagc acagccugug cuaccagaag     1740

uaccucgucu acaacgagcu gaccaaggug cgcuacauca acgaccaggg gaagaccucc     1800

uacuucagcg gccaggagaa ggagcagauc uucaacgacc uguucaagca gaagcggaag     1860

gucaagaaga aggaccucga gcuguuccug cgcaacaugu cccacgugga gagccccacg     1920

aucgaggggc uggaggacuc cuucaacagc uccuacagca ccuaccacga ccuccugaag     1980

gugggcauca agcaggagau ccuggacaac cccgucaaca ccgagaugcu cgagaacauc     2040

gugaagaucc ugaccguguu cgaggacaag cggaugauca aggagcagcu gcagcaguuc     2100

uccgacgucc ucgacggcgu ggugcugaag aagcuggagc gccggcacua cacggggugg     2160

ggccgccuca gcgccaagcu gcugaugggg auccgggaca agcaguccca ccucaccauc     2220

cuggacuacc ugaugaacga cgacggccuc aaccgcaacc ugaugcagcu gaucaacgac     2280

agcaaccucu ccuucaagag caucaucgag aaggagcagg ucaccaccgc cgacaaggac     2340

auccagagca ucguggccga ccuggcgggg uccccggcca ucaagaaggg cauccugcag     2400

agccucaaga ucguggacga gcuggucucc gugaugggcu acccgcccca gacgaucgug     2460

gucgagaugg cccgggagaa ccagaccacc gggaagggca agaacaacag ccgcccccgg     2520

uacaaguccc uggagaaggc caucaaggag uucgggagcc agauccucaa ggagcacccc     2580

accgacaacc aggagcugcg caacaaccgg cuguaccucu acuaccugca gaacggcaag     2640

gacauguaca cgggccagga ccuggacauc cacaaccucu ccaacuacga caucgaccac     2700

aucgugcccc agagcuucau caccgacaac uccaucgaca accuggugcu gaccagcucc     2760

gccgggaacc gcgagaaggg cgacgacguc ccgccccucg agaucgugcg gaagcgcaag     2820

guguucuggg agaagcugua ccaggggaac cugaugagca agcgcaaguu cgacuaccuc     2880

accaaggcgg agcggggcgg gcugaccgag gccgacaagg cccgcuucau ccaccggcag     2940

cuggucgaga cgcgccagau caccaagaac guggccaaca uccuccacca gcgguucaac     3000

uacgagaagg acgaccacgg caacaccaug aagcaggucc gcaucgugac ccugaagucc     3060

gcgcugguga gccaguuccg gaagcaguuc cagcucuaca aggugcgcga cgucaacgac     3120

uaccaccacg cccacgacgc cuaccugaac ggcguggugg ccaacacgcu gcucaagguc     3180

uacccccagc uggagcccga guucguguac ggggacuacc accaguucga cugguucaag     3240

gccaacaagg cgaccgccaa gaagcaguuc uacaccaaca ucaugcuguu cuucgcccag     3300

aaggaccgga ucaucgacga gaacggcgag auccucuggg acaagaagua ccuggacacc     3360

gugaagaagg ucauguccua ccgccagaug aacaucguga agaagacgga gauccagaag     3420

ggggaguuca gcaaggccac caucaagccc aagggcaacu ccagcaagcu gaucccccgg     3480

aagaccaacu gggacccgau gaaguacggg ggccucgaca gccccaacau ggccuacgcg     3540

guggucaucg aguacgccaa gggcaagaac aagcuggugu ucgagaagaa gaucauccgc     3600

gugaccauca uggagcggaa ggccuucgag aaggacgaga aggccuuccu ggaggagcag     3660

ggguaccgcc agcccaaggu ccucgccaag cugccgaagu acacgcugua cgagugcgag     3720

gagggccggc gccggaugcu cgcguccgcc aacgaggccc agaaggggaa ccagcagguc     3780

cugcccaacc accuggugac ccuccugcac cacgccgcga acugcgaggu gagcgacggc     3840

aagucccugg acuacaucga gagcaaccgc gagauguucg ccgagcuccu ggcccacgug     3900

uccgaguucg ccaagcggua cacccuggcc gaggcgaacc ucaacaagau caaccagcug     3960

uucgagcaga acaaggaggg ggacaucaag gccaucgccc agagcuucgu ggaccugaug     4020

gccuucaacg ccaugggcgc gcccgccucc uucaaguucu ucgagaccac gaucgagcgc     4080

aagcgguaca acaaccucaa ggagcugcug aacagcacca ucaucuacca guccaucacc     4140

ggccucuacg agagccgcaa gcgccuggac gacaagcggc ccgccgccac caagaaggcc     4200

gggcaggcga agaagaagaa guaaggacua gucccuguuc ccagagccca cuuuuuuuuc     4260

uuuuuuugaa auaaaauagc cugucuuuca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4364


<210>  2498
<211>  4397
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2498
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaagaag      180

cccuacagca ucggccugga caucgggacc aacuccgugg gcugggccgu ggucaccgac      240

gacuacaagg ugcccgccaa gaagaugaag gugcucggga acacggacaa gagccacauc      300

gagaagaacc ugcugggcgc ccuccuguuc gacuccggca acaccgccga ggaccggcgc      360

cugaagcgga ccgcgcgccg gcgcuacacc cggcgccgga accgcauccu cuaccugcag      420

gagaucuuca gcgaggagau ggggaagguc gacgacuccu ucuuccaccg gcuggaggac      480

agcuuccucg ugacggagga caagcgcggc gagcggcacc ccaucuucgg gaaccuggag      540

gaggagguga aguaccacga gaacuucccg accaucuacc accuccgcca guaccuggcc      600

gacaaccccg agaaggucga ccugcggcug guguaccucg cccuggccca caucaucaag      660

uuccgcggcc acuuccugau cgaggggaag uucgacaccc ggaacaacga cgugcagcgc      720

cucuuccagg aguuccuggc ggucuacgac aacaccuucg agaacuccag ccugcaggag      780

cagaacgucc agguggagga gauccucacg gacaagaucu ccaagagcgc caagaaggac      840

cgggugcuga agcuguuccc caacgagaag uccaacggcc gcuucgccga guuccucaag      900

cugaucgugg gcaaccaggc cgacuucaag aagcacuucg agcuggagga gaaggccccg      960

cuccaguuca gcaaggacac cuacgaggag gagcuggagg ugcugcucgc gcagaucggg     1020

gacaacuacg ccgagcuguu ccuguccgcc aagaagcucu acgacagcau ccugcugucc     1080

ggcauccuca ccgucaccga cguggggacg aaggccccgc ugagcgccuc caugauccag     1140

cgguacaacg agcaccagau ggaccuggcg cagcucaagc aguucauccg ccagaagcug     1200

agcgacaagu acaacgaggu guucuccgac gucagcaagg acggcuacgc cggcuacauc     1260

gacgggaaga ccaaccagga ggccuucuac aaguaccuga agggccuccu gaacaagauc     1320

gaggggagcg gcuacuuccu ggacaagauc gagcgcgagg acuuccuccg gaagcagcgc     1380

accuucgaca acggguccau cccccaccag auccaccucc aggagaugcg ggccaucauc     1440

cgccggcagg ccgaguucua ccccuuccug gcggacaacc aggaccgcau cgagaagcug     1500

cugaccuucc ggauccccua cuacgugggc ccgcuggccc gcggcaagag cgacuucgcc     1560

uggcuguccc gcaagagcgc cgacaagauc acgcccugga acuucgacga gaucguggac     1620

aaggagucca gcgccgaggc guucaucaac cggaugacca acuacgaccu cuaccugccc     1680

aaccagaagg uccugcccaa gcacucccuc cuguacgaga aguucaccgu guacaacgag     1740

cugaccaagg ucaaguacaa gacggagcag gggaagaccg ccuucuucga cgccaacaug     1800

aagcaggaga ucuucgacgg cguguucaag guguaccgga aggucaccaa ggacaagcuc     1860

auggacuucc uggagaagga guucgacgag uuccgcaucg uggaccugac cgggcucgac     1920

aaggagaaca agguguucaa cgccagcuac ggcacguacc acgaccugug caagauccug     1980

gacaaggacu uccucgacaa cuccaagaac gagaagaucc uggaggacau cguccugacc     2040

cucacccugu ucgaggaccg ggagaugauc cgcaagcggc uggagaacua cagcgaccuc     2100

cugaccaagg agcaggucaa gaagcuggag cgccggcacu acacggggug gggccgccuc     2160

uccgcggagc ugauccacgg cauccggaac aaggagagcc gcaagaccau ccuggacuac     2220

cucaucgacg acgggaacuc caaccgcaac uucaugcagc ugaucaacga cgacgcccug     2280

agcuucaagg aggagaucgc caaggcccag gugaucggcg agaccgacaa ccucaaccag     2340

gugguguccg acaucgccgg gagcccggcg aucaagaagg gcauccugca gucccugaag     2400

aucguggacg agcucgucaa gaucaugggg caccagcccg agaacaucgu gguggagaug     2460

gcccgggaga accaguucac caaccagggc cgccggaaca gccagcagcg ccugaagggc     2520

cugacggacu ccaucaagga guucgggagc cagauccuca aggagcaccc cgucgagaac     2580

agccagcugc agaacgaccg gcuguuccuc uacuaccugc agaacggccg cgacauguac     2640

accggggagg agcuggacau cgacuaccuc ucccaguacg acaucgacca caucaucccc     2700

caggccuuca ucaaggacaa cagcaucgac aaccgggugc ugaccuccag caaggagaac     2760

cgcggcaagu ccgacgacgu gccgagcaag gacgucgugc ggaagaugaa guccuacugg     2820

agcaagcugc ucuccgccaa gcugaucacc cagcgcaagu ucgacaaccu gacgaaggcc     2880

gagcggggcg ggcucaccga cgacgacaag gcgggcuuca ucaagcgcca gcugguggag     2940

acccggcaga ucaccaagca cgucgcccgc auccuggacg agcgguucaa caccgagacg     3000

gacgagaaca acaagaagau ccgccaggug aagaucguga cccucaagag caaccugguc     3060

uccaacuucc ggaaggaguu cgagcuguac aaggugcgcg agaucaacga cuaccaccac     3120

gcccacgacg ccuaccucaa cgccgugauc gggaaggcgc ugcugggcgu cuacccccag     3180

cucgagcccg aguucgugua cggggacuac ccccacuucc acggccacaa ggagaacaag     3240

gccaccgcca agaaguucuu cuacagcaac aucaugaacu ucuucaagaa ggacgacgug     3300

cggaccgaca agaacggcga gaucaucugg aagaaggacg agcacaucuc caacaucaag     3360

aagguccuga gcuaccccca ggugaacauc gugaagaagg ucgaggagca gaccgggggc     3420

uucuccaagg agagcauccu gcccaagggg aacuccgaca agcucauccc ccgcaagacg     3480

aagaaguucu acugggacac caagaaguac ggcggguucg acagccccau cguggccuac     3540

agcauccugg ugaucgcgga caucgagaag ggcaagucca agaagcugaa gaccgucaag     3600

gcccucgugg gcgugacgau cauggagaag augaccuucg agcgggaccc ggucgccuuc     3660

cuggagcgca agggguaccg caacgugcag gaggagaaca ucaucaagcu gcccaaguac     3720

agccucuuca agcuggagaa cggccggaag cgccugcucg ccuccgcccg ggagcugcag     3780

aaggggaacg agaucgugcu gcccaaccac cucggcaccc ugcuguacca cgcgaagaac     3840

auccacaagg ucgacgagcc gaagcaccuc gacuacgugg acaagcacaa ggacgaguuc     3900

aaggagcugc uggacguggu cagcaacuuc uccaagaagu acacccucgc cgaggggaac     3960

cuggagaaga ucaaggagcu guacgcccag aacaacggcg aggaccucaa ggagcuggcc     4020

agcuccuuca ucaaccugcu cacguucacc gccaucggcg cgcccgccac cuucaaguuc     4080

uucgacaaga acaucgaccg caagcgguac accagcacga ccgagauccu gaacgccacc     4140

cugauccacc aguccaucac cgggcucuac gagacgcgca ucgaccugaa caagcugggc     4200

ggggacaagc ggcccgccgc caccaagaag gcgggccagg ccaagaagaa gaaguaagga     4260

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4320

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aagaauu                                                    4397


<210>  2499
<211>  3530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2499
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgcagacc      180

accaaccuga gcuacauccu cggccuggac cuggggaucg ccuccguggg cugggccgug      240

gucgagauca acgagaacga ggaccccauc gggcucaucg acgugggcgu gcggaucuuc      300

gagcgcgccg agguccccaa gacgggcgag agccuggccc ugucccggcg ccucgcgcgg      360

agcacccgcc ggcugauccg ccggcgcgcc caccggcugc uccuggccaa gcgcuuccug      420

aagcgggagg ggauccucuc caccaucgac cuggagaagg gccugcccaa ccaggccugg      480

gagcuccgcg uggcggggcu ggagcggcgc cugagcgcca ucgagugggg cgccgugcuc      540

cugcaccuga ucaagcaccg gggguaccuc uccaagcgca agaacgagag ccagaccaac      600

aacaaggagc ugggcgcccu gcucuccggc gucgcccaga accaccagcu gcugcagagc      660

gacgacuacc ggacgccggc ggagcucgcc cugaagaagu ucgccaagga ggaggggcac      720

auccgcaacc agcggggcgc cuacacccac accuucaacc gccuggaccu ccuggccgag      780

cugaaccucc uguucgcgca gcagcaccag uucgggaacc cccacugcaa ggagcacauc      840

cagcaguaca ugaccgagcu gcucaugugg cagaagcccg cccuguccgg cgaggccauc      900

cugaagaugc ucggcaagug cacgcacgag aagaacgagu ucaaggccgc caagcacacc      960

uacagcgcgg agcgcuucgu guggcugacc aagcugaaca accuccggau ccuggaggac     1020

ggggccgagc gcgcccugaa cgaggaggag cggcagcucc ugaucaacca ccccuacgag     1080

aaguccaagc ugaccuacgc ccaggugcgc aagcuccugg gccugagcga gcaggccauc     1140

uucaagcacc uccgguacuc caaggagaac gcggagagcg ccacguucau ggagcucaag     1200

gccuggcacg ccauccgcaa ggcgcuggag aaccaggggc ugaaggacac cuggcaggac     1260

cucgccaaga agccggaccu gcuggacgag aucggcaccg ccuucucccu cuacaagacc     1320

gacgaggaca uccagcagua ccugacgaac aaggucccca acagcgugau caacgcccug     1380

cucguguccc ugaacuucga caaguucauc gagcugagcc ucaagagccu gcggaagauc     1440

cugccccuca uggagcaggg gaagcgcuac gaccaggccu gccgggagau cuacggccac     1500

cacuacggcg aggcgaacca gaagaccucc cagcugcugc ccgccauccc ggcccaggag     1560

auccgcaacc ccgucgugcu ccggacccug agccaggccc gcaaggugau caacgccauc     1620

auccggcagu acgggucccc cgcgcgcguc cacaucgaga ccggccggga gcugggcaag     1680

agcuucaagg agcgccggga gauccagaag cagcaggagg acaaccgcac gaagcgggag     1740

uccgccgugc agaaguucaa ggagcuguuc agcgacuucu ccagcgagcc caaguccaag     1800

gacauccuga aguuccgccu guacgagcag cagcacggga agugccucua cagcgggaag     1860

gagaucaaca uccaccgccu gaacgagaag ggcuacgugg agaucgacca cgcccugccg     1920

uucucccgga ccugggacga cagcuucaac aacaaggucc ucgugcuggc cuccgagaac     1980

cagaacaagg gcaaccagac ccccuacgag uggcugcagg ggaagaucaa cagcgagcgc     2040

uggaagaacu ucguggcccu cguccugggc ucccagugca gcgcggccaa gaagcagcgg     2100

cugcucaccc aggugaucga cgacaacaag uucaucgacc gcaaccugaa cgacacgcgg     2160

uacaucgccc gcuuccuguc caacuacauc caggagaacc uccugcuggu cgggaagaac     2220

aagaagaacg uguucacccc caacggccag aucaccgccc uccugcggag ccgcuggggg     2280

cugaucaagg cgcgggagaa caacaaccgc caccacgccc ucgacgccau cguggucgcc     2340

ugcgccaccc ccuccaugca gcagaagauc acgcgguuca uccgcuucaa ggaggugcac     2400

ccguacaaga ucgagaaccg guacgagaug guggaccagg agagcggcga gaucaucagc     2460

ccccacuucc ccgagcccug ggcguacuuc cgccaggagg ucaacauccg gguguucgac     2520

aaccacccgg acaccgugcu gaaggagaug cugcccgacc gcccccaggc caaccaccag     2580

uucguccagc cccucuucgu gucccgggcc ccgacccgca agaugagcgg ccaggggcac     2640

auggagacca ucaaguccgc caagcggcug gccgagggca ucagcgugcu gcgcaucccc     2700

cucacgcagc ugaagcccaa ccugcucgag aacaugguca acaaggagcg ggagccggcg     2760

cuguacgccg ggcugaaggc ccgccucgcc gaguucaacc aggaccccgc caaggcguuc     2820

gccacccccu ucuacaagca gggcggccag caggucaagg ccauccgcgu ggagcagguc     2880

cagaaguccg gggugcuggu gcgggagaac aacggcgugg ccgacaacgc gagcaucgug     2940

cgcaccgacg uguucaucaa gaacaacaag uucuuccugg uccccaucua caccuggcag     3000

gucgccaagg ggauccuccc gaacaaggcc aucguggccc acaagaacga ggacgagugg     3060

gaggagaugg acgagggcgc caaguucaag uucucccugu uccccaacga ccugguggag     3120

cucaagacga agaaggagua cuucuucggg uacuacaucg gccuggaccg ggcgaccggc     3180

aacaucagcc ugaaggagca cgacggggag aucuccaagg gcaaggacgg gguguaccgc     3240

gugggcguca agcucgcccu gagcuucgag aaguaccagg uggacgagcu ggggaagaac     3300

cggcagaucu gccgccccca gcagcggcag cccgugcgca agcggccggc cgccaccaag     3360

aaggccggcc aggcgaagaa gaagaaguaa ggacuagucc cuguucccag agcccacuuu     3420

uuuuucuuuu uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                3530


<210>  2500
<211>  3608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2500
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcggccgcc      180

uucaagccca accccaucaa cuacauccug ggccucgaca ucgggaucgc cagcgugggc      240

ugggccaugg uggagaucga cgaggacgag aaccccaucu gccugaucga ccuggggguc      300

cggguguucg agcgcgcgga ggugccgaag accggcgacu cccucgccau ggcccggcgc      360

cuggcccgga gcguccgccg gcugacccgc cggcgcgcgc accggcuccu gcgcgcccgg      420

cgccugcuca agcgggaggg cgugcugcag gccgccgacu ucgacgagaa cgggcugauc      480

aagucccucc ccaacacgcc cuggcagcug cgcgccgcgg cccuggaccg gaagcucacg      540

ccccuggagu ggagcgccgu gcugcuccac cugaucaagc accgcggcua ccugucccag      600

cggaagaacg agggggagac cgccgacaag gagcucggcg cccugcugaa gggggucgcg      660

gacaacgccc acgcccucca gaccggcgac uuccgcaccc cggccgagcu ggcccugaac      720

aaguucgaga aggagagcgg ccacauccgg aaccagcgcg gggacuacuc ccacaccuuc      780

agccgcaagg accuccaggc ggagcugauc cuccucuucg agaagcagaa ggaguucggc      840

aacccccacg uguccggggg ccugaaggag ggcaucgaga cccugcucau gacccagcgg      900

cccgcccuga gcggggacgc cgugcagaag augcugggcc acugcacguu cgagcccgcc      960

gagccgaagg ccgcgaagaa caccuacacc gccgagcgcu ucaucuggcu caccaagcug     1020

aacaaccugc ggauccucga gcaggggucc gagcgccccc ugacggacac cgagcgggcc     1080

acccugaugg acgagcccua ccgcaagagc aagcucaccu acgcccaggc gcggaagcug     1140

cugggccucg aggacacggc cuucuucaag gggcugcgcu acggcaagga caacgccgag     1200

gccuccaccc ugauggagau gaaggccuac cacgcgauca gccgggcccu cgagaaggag     1260

ggccugaagg acaagaaguc cccgcugaac cugagccccg agcuccagga cgagaucggg     1320

accgccuucu cccuguucaa gaccgacgag gacaucacgg gccgccugaa ggaccggauc     1380

cagcccgaga uccucgaggc ccugcugaag cacaucagcu ucgacaaguu cguccagauc     1440

ucccucaagg cccugcgccg gaucgugccc cugauggagc aggggaagcg cuacgacgag     1500

gcgugcgccg agaucuacgg cgaccacuac gggaagaaga acaccgagga gaagaucuac     1560

cugcccccga uccccgccga cgagauccgg aaccccgugg uccugcgcgc ccugagccag     1620

gcccggaagg ugaucaacgg cgugguccgc cgguacggca gccccgcgcg cauccacauc     1680

gagaccgccc gcgagguggg gaaguccuuc aaggaccgga aggagaucga gaagcgccag     1740

gaggagaacc ggaaggaccg cgagaaggcc gccgcgaagu uccgggagua cuucccgaac     1800

uucgugggcg agcccaagag caaggacauc cucaagcugc gccuguacga gcagcagcac     1860

gggaagugcc ucuacuccgg caaggagauc aaccuggggc ggcugaacga gaagggcuac     1920

gucgagaucg accacgcccu ccccuucagc cgcaccuggg acgacuccuu caacaacaag     1980

gugcuggugc ugggcagcga gaaccagaac aaggggaacc agacgcccua cgaguacuuc     2040

aacggcaagg acaacucccg ggaguggcag gaguucaagg cccgcgucga gaccagccgg     2100

uucccgcgcu ccaagaagca gcggauccuc cugcagaagu ucgacgagga cggguucaag     2160

gagcgcaacc ugaacgacac ccgguacgug aaccgcuucc ucugccaguu cgucgccgac     2220

cggaugcgcc ugaccggcaa gggcaagaag cggguguucg ccagcaacgg gcagaucacg     2280

aaccugcucc gcggcuucug ggggcugcgg aaggugcgcg cggagaacga ccgccaccac     2340

gcccuggacg ccgucguggu ggccugcucc accgucgcca ugcagcagaa gaucacccgg     2400

uucgugcgcu acaaggagau gaacgcguuc gacggcaaga ccaucgacaa ggagacgggg     2460

gaggugcucc accagaagac ccacuucccc cagcccuggg aguucuucgc ccaggagguc     2520

augauccggg uguucggcaa gccggacggc aagcccgagu ucgaggaggc cgacaccccc     2580

gagaagcugc gcacccugcu cgccgagaag cugagcuccc ggcccgaggc cgugcacgag     2640

uacgucacgc cgcuguucgu gagccgcgcg cccaaccgga agauguccgg gcagggccac     2700

auggagaccg ucaagagcgc caagcgccuc gacgaggggg uguccgugcu gcgggugccc     2760

cugacccagc ucaagcugaa ggaccuggag aagaugguca accgcgagcg ggagcccaag     2820

cucuacgagg cccugaaggc ccgccuggag gcgcacaagg acgacccggc caaggccuuc     2880

gccgagcccu ucuacaagua cgacaaggcc ggcaaccgga cccagcaggu caaggcggug     2940

cgcguggagc agguccagaa gaccggggug ugggugcgga accacaacgg caucgccgac     3000

aacgccacga uggugcgcgu ggacgucuuc gagaagggcg acaaguacua ccucgugccc     3060

aucuacagcu ggcaggucgc caaggggauc cugcccgacc gggccguggu gcagggcaag     3120

gacgaggagg acuggcagcu gaucgacgac uccuucaacu ucaaguucag ccuccacccg     3180

aacgaccugg uggaggucau caccaagaag gcgcgcaugu ucggguacuu cgccagcugc     3240

caccggggca ccgggaacau caacauccgc auccacgacc uggaccacaa gaucggcaag     3300

aacggcaucc ucgaggggau cggcgugaag accgcccugu ccuuccagaa guaccagauc     3360

gacgagcugg ggaaggagau ccgccccugc cggcucaaga agcgcccgcc cgugcggaag     3420

cgccccgccg ccacgaagaa ggcgggccag gccaagaaga agaaguaagg acuagucccu     3480

guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaagaauu                                                              3608


<210>  2501
<211>  3614
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2501
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgaaguac      180

cacgugggca ucgacguggg gaccuucagc gucggccugg ccgccaucga gguggacgac      240

gccgggaugc ccaucaagac ccugucccug gugagccaca uccacgacuc cggccuggac      300

cccgacgaga ucaagagcgc cgucacgcgg cucgcgucca gcggcaucgc ccgccggacc      360

cgccggcugu accgccggaa gcgccggcgc cugcagcagc ucgacaaguu cauccagcgg      420

cagggguggc ccgugaucga gcuggaggac uacuccgacc cgcuguaccc cuggaaggug      480

cgcgccgagc ucgccgcgag cuacaucgcc gacgagaagg agcggggcga gaagcugucc      540

gucgcccugc gccacaucgc ccggcaccgc ggguggcgga accccuacgc caaggugagc      600

ucccucuacc ugcccgacgg cccgagcgac gcguucaagg ccauccgcga ggagaucaag      660

cgggccuccg ggcagcccgu gcccgagacc gccaccgucg gccagauggu gacgcugugc      720

gagcucggca cccugaagcu gcgcggggag ggcggggugc ucagcgcccg gcugcagcag      780

uccgacuacg cgcgcgagau ccaggagauc ugccgcaugc aggagaucgg ccaggagcug      840

uaccggaaga ucaucgacgu cguguucgcc gccgagagcc ccaagggcuc cgccagcucc      900

cgcgugggga aggacccgcu ccagcccggc aagaaccggg cccugaaggc gagcgacgcc      960

uuccagcgcu accggaucgc cgcccugauc gggaaccucc gcguccgggu ggacggcgag     1020

aagcgcaucc ugagcgugga ggagaagaac cuggucuucg accaccucgu gaaccugacc     1080

cccaagaagg agcccgagug ggugaccauc gccgagaucc uggggaucga ccggggccag     1140

cucaucggca cggcgaccau gaccgacgac ggggagcgcg ccggcgcccg gccgcccacc     1200

cacgacacga accgcuccau cgucaacagc cggaucgcgc cccuggugga cugguggaag     1260

accgccuccg cccuggagca gcacgccaug gucaaggccc ucagcaacgc cgagguggac     1320

gacuucgacu cccccgaggg ggcgaaggug caggccuucu ucgccgaccu ggacgacgac     1380

guccacgcca agcuggacag ccuccaccuc ccggugggcc gcgccgcgua cuccgaggac     1440

acccuggugc ggcucacccg ccggaugcug agcgacgggg ucgaccugua cacggcccgc     1500

cuccaggagu ucggcaucga gcccuccugg accccgccca cgccccggau cggcgagccc     1560

guggggaacc ccgccgugga ccgcguccug aagaccguga gccgcuggcu ggaguccgcc     1620

accaagaccu ggggcgcccc cgagcgggug aucaucgagc acguccgcga gggguucgug     1680

accgagaagc gggcgcgcga gauggacggc gacaugcggc gccgggccgc ccgcaacgcc     1740

aagcuguucc aggagaugca ggagaagcug aacgugcagg ggaagccgag ccgggcggac     1800

cuguggcgcu accaguccgu ccagcggcag aacugccagu gcgccuacug cggcagcccc     1860

aucaccuucu ccaacagcga gauggaccac aucgugcccc gcgccggcca gggguccacg     1920

aacacccggg agaaccucgu ggccgucugc caccgcugca accagagcaa gggcaacacc     1980

ccguucgcca ucugggcgaa gaacaccucc aucgaggggg ugagcgugaa ggaggccguc     2040

gagcggacgc gccacugggu gaccgacacc ggcaugcgga gcaccgacuu caagaaguuc     2100

acgaaggccg uggucgagcg cuuccagcgg gccaccaugg acgaggagau cgacgcccgc     2160

uccauggaga gcguggcgug gauggccaac gagcugcggu cccgcguggc ccagcacuuc     2220

gccagccacg gcaccaccgu ccgcguguac cggggguccc ugacggccga ggcgcgccgg     2280

gccagcggca ucuccgggaa gcugaaguuc uucgacggcg ugggcaagag ccgccucgac     2340

cggcgccacc acgccaucga cgccgcgguc aucgccuuca ccuccgacua cguggccgag     2400

acccuggccg ugcggagcaa ccugaagcag ucccaggccc accgccagga ggcgccccag     2460

uggcgggagu ucaccgggaa ggacgccgag caccgcgccg ccuggcgggu cuggugccag     2520

aagauggaga agcucagcgc ccugcugacc gaggaccucc gcgacgaccg gguggugguc     2580

auguccaacg ugcgccugcg gcugggcaac gggagcgcgc acaaggagac gaucggcaag     2640

cucuccaagg ugaagcugag cucccagcug agcgucagcg acaucgacaa ggccuccagc     2700

gaggcccucu ggugcgcccu gacccgcgag cccggguucg accccaagga gggccugccg     2760

gccaaccccg agcggcacau ccgcgugaac gggacccacg uguacgcggg cgacaacauc     2820

ggccucuucc ccgucuccgc cgggagcauc gcccugcggg gcggguacgc cgagcugggc     2880

uccagcuucc accacgcccg cguguacaag aucaccuccg ggaagaagcc cgcguucgcc     2940

augcuccgcg uguacacgau cgaccugcug ccguaccgga accaggaccu cuucagcguc     3000

gagcugaagc cccagaccau guccaugcgc caggccgaga agaagcugcg ggacgcccuc     3060

gcgaccggca acgccgagua ccugggcugg cugguggugg acgacgagcu cgucguggac     3120

accagcaaga ucgccacgga ccaggugaag gccgucgagg ccgagcuggg gaccauccgc     3180

cgguggcgcg uggacggcuu cuucuccccc agcaagcugc ggcuccgccc ccugcagaug     3240

uccaaggagg ggaucaagaa ggagagcgcg ccggagcugu ccaagaucau cgaccggccc     3300

ggcuggcucc ccgccgucaa caagcuguuc agcgacggga acgugaccgu gguccgccgg     3360

gacucccugg gccgcgugcg gcucgagagc accgcccacc uccccgugac guggaagguc     3420

cagaagcgcc cggccgccac caagaaggcg ggccaggcca agaagaagaa guaaggacua     3480

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     3540

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaag aauu                                                       3614


<210>  2502
<211>  3314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2502
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcggcccgg      180

auccuggccu ucgacaucgg caucagcucc aucggguggg ccuucagcga gaacgacgag      240

cucaaggacu gcggcgugcg caucuucacc aagguggaga accccaagac cggggagucc      300

cuggcccugc cccggcgccu cgcgcggagc gcccgcaagc ggcuggcccg ccggaaggcc      360

cgccugaacc accucaagca ccugaucgcg aacgaguuca agcugaacua cgaggacuac      420

caguccuucg acgagagccu cgccaaggcc uacaagggcu cccugaucag ccccuacgag      480

cugcgguucc gcgcccucaa cgagcugcug uccaagcagg acuucgcccg ggucauccuc      540

cacaucgcga agcgccgggg cuacgacgac aucaagaaca gcgacgacaa ggagaagggg      600

gccauccuga aggccaucaa gcagaacgag gagaagcugg ccaacuacca guccgugggc      660

gaguaccucu acaaggagua cuuccagaag uucaaggaga acagcaagga guucacgaac      720

gugcgcaaca agaaggaguc cuacgagcgg ugcaucgccc agagcuuccu gaaggacgag      780

cugaagcuca ucuucaagaa gcagcgcgag uucggguucu ccuucagcaa gaaguucgag      840

gaggaggucc uguccguggc guucuacaag cgggcccuga aggacuucag ccaccucgug      900

ggcaacugcu ccuucuucac cgacgagaag cgcgccccga agaacagccc ccuggccuuc      960

auguucgucg cccugacccg gaucaucaac cuccugaaca accugaagaa caccgagggg     1020

auccucuaca cgaaggacga ccugaacgcg cugcucaacg aggugcugaa gaacggcacc     1080

cugaccuaca agcagaccaa gaagcuccug ggccugagcg acgacuacga guucaagggg     1140

gagaagggca cguacuucau cgaguucaag aaguacaagg aguucaucaa ggcccucggg     1200

gagcacaacc ugucccagga cgaccugaac gagaucgcca aggacaucac ccucaucaag     1260

gacgagauca agcugaagaa ggcccuggcc aaguacgacc ucaaccagaa ccagaucgac     1320

agccugucca agcuggaguu caaggaccac cucaacauca gcuucaaggc gcugaagcug     1380

gugacgcccc ugaugcucga gggcaagaag uacgacgagg ccugcaacga gcugaaccug     1440

aaggucgcca ucaacgagga caagaaggac uuccuccccg ccuucaacga gaccuacuac     1500

aaggacgagg ugaccaaccc ggugguccug cgcgcgauca aggaguaccg caaggugcug     1560

aacgcccucc ugaagaagua cggcaaggug cacaagauca acaucgagcu ggcccgggag     1620

gucgggaaga accacuccca gcgcgccaag aucgagaagg agcagaacga gaacuacaag     1680

gccaagaagg acgcggagcu cgagugcgag aagcugggcc ugaagaucaa cagcaagaac     1740

auccucaagc ugcggcuguu caaggagcag aaggaguucu gcgccuacuc cggggagaag     1800

aucaagauca gcgaccucca ggacgagaag augcuggaga ucgaccacau cuaccccuac     1860

ucccgcagcu ucgacgacuc cuacaugaac aaggugcugg uguucaccaa gcagaaccag     1920

gagaagcuca accagacccc cuucgaggcc uucggcaacg acagcgccaa guggcagaag     1980

aucgaggucc uggccaagaa ccugcccacc aagaagcaga agcggauccu cgacaagaac     2040

uacaaggaca aggagcagaa gaacuucaag gaccgcaacc ugaacgacac gcgguacauc     2100

gcgcgccugg ugcucaacua caccaaggac uaccuggacu uccugccgcu cuccgacgac     2160

gagaacacca agcugaacga cacccagaag gggagcaagg ugcacgucga ggccaagucc     2220

ggcaugcuga cgagcgcccu ccggcacacc uggggcuucu ccgccaagga ccgcaacaac     2280

caccuccacc acgccaucga cgcggugauc aucgccuacg ccaacaacag caucgucaag     2340

gccuucuccg acuucaagaa ggagcaggag agcaacagcg cggagcugua cgccaagaag     2400

aucuccgagc uggacuacaa gaacaagcgg aaguucuucg agcccuucag cggguuccgc     2460

cagaaggugc uggacaagau cgacgagauc uucgugucca agcccgagcg gaagaagccc     2520

agcggcgccc ugcacgagga gaccuuccgc aaggaggagg aguucuacca guccuacggg     2580

ggcaaggagg ggguccucaa ggcccuggag cugggcaaga uccggaaggu gaacggcaag     2640

aucgugaaga acggggacau guuccgcguc gacaucuuca agcacaagaa gaccaacaag     2700

uucuacgccg ugccgaucua cacgauggac uucgcgcuca aggugcugcc caacaaggcc     2760

gucgcccgga gcaagaaggg cgagaucaag gacuggaucc ugauggacga gaacuacgag     2820

uucugcuucu cccucuacaa ggacagccug auccugaucc agaccaagga caugcaggag     2880

cccgaguucg uguacuacaa cgccuucacc uccagcaccg ugucccucau cgucagcaag     2940

cacgacaaca aguucgagac gcuguccaag aaccagaaga uccuguucaa gaacgccaac     3000

gagaaggagg ugaucgccaa gagcaucggg auccagaacc ucaagguguu cgagaaguac     3060

aucgucuccg cgcugggcga ggugaccaag gccgaguucc gccagcggga ggacuucaag     3120

aagaagcgcc ccgccgccac caagaaggcg gggcaggcca agaagaagaa guaaggacua     3180

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     3240

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3300

aaaaaaaaag aauu                                                       3314


<210>  2503
<211>  3725
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2503
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgagcgac      180

cuggugcucg gccuggacau cgggaucggc uccguggggg ucggcauccu gaacaaggug      240

accggcgaga ucauccacaa gaacagccgg aucuuccccg ccgcccaggc cgagaacaac      300

cucgugcgcc ggaccaaccg ccaggggcgg cgccuggccc ggcgcaagaa gcaccggcgc      360

guccggcuga accgccucuu cgaggagucc ggccugauca cggacuucac caagaucagc      420

aucaaccuga accccuacca gcuccgggug aaggggcuga ccgacgagcu guccaacgag      480

gagcuguuca ucgcgcugaa gaacauggug aagcaccgcg gcaucagcua ccuggacgac      540

gccuccgacg acgggaacag cuccgucggc gacuacgccc agaucgugaa ggagaacagc      600

aagcagcucg agaccaagac gcccggccag auccagcugg agcgguacca gaccuacggg      660

cagcugcgcg gcgacuucac cguggagaag gacgggaaga agcaccggcu caucaacguc      720

uucccgaccu ccgccuaccg cagcgaggcg cugcggaucc ugcagacgca gcaggaguuc      780

aacccccaga ucaccgacga guucaucaac cgcuaccucg agauccugac cggcaagcgg      840

aaguacuacc acggccccgg gaacgagaag ucccgcaccg acuacggccg cuaccggacg      900

agcggggaga cccuggacaa caucuucggc auccucaucg ggaagugcac cuucuacccc      960

gacgaguucc gcgccgccaa ggccuccuac accgcccagg aguucaaccu gcugaacgac     1020

cucaacaacc ugacggugcc gaccgagacc aagaagcuga gcaaggagca gaagaaccag     1080

aucaucaacu acgugaagaa cgagaaggcg augggccccg ccaagcuguu caaguacauc     1140

gccaagcucc uguccugcga cgucgccgac aucaagggcu accggaucga caagagcggg     1200

aaggccgaga uccacaccuu cgaggcguac cgcaagauga agacgcucga gacccuggac     1260

aucgagcaga uggaccggga gacccuggac aagcucgccu acgugcugac ccugaacacg     1320

gagcgcgagg gcauccagga ggcccucgag cacgaguucg ccgacggguc cuucagccag     1380

aagcaggucg acgagcuggu gcaguuccgg aaggccaaca gcuccaucuu cggcaagggg     1440

uggcacaacu ucagcgugaa gcugaugaug gagcucaucc ccgagcugua cgagaccucc     1500

gaggagcaga ugaccauccu gacccgccuc ggcaagcaga agacgaccag cuccagcaac     1560

aagaccaagu acaucgacga gaagcugcug accgaggaga ucuacaaccc cguggucgcg     1620

aaguccgugc ggcaggccau caagaucgug aacgccgcca ucaaggagua cggcgacuuc     1680

gacaacaucg ucaucgagau ggcccgcgag acgaacgagg acgacgagaa gaaggcgauc     1740

cagaagaucc agaaggccaa caaggacgag aaggacgccg ccaugcucaa ggcggccaac     1800

caguacaacg ggaaggccga gcugccgcac agcguguucc acggccacaa gcagcuggcc     1860

accaagaucc ggcuguggca ccagcagggg gagcgcugcc ucuacaccgg caagaccauc     1920

uccauccacg accugaucaa caacagcaac caguucgagg ucgaccacau ccugccccuc     1980

uccaucacgu ucgacgacag ccuggccaac aaggugcugg uguacgcgac cgccaaccag     2040

gagaaggggc agcggacccc cuaccaggcc cucgacucca uggacgacgc cuggagcuuc     2100

cgcgagcuga aggccuucgu ccgggagucc aagacccuga gcaacaagaa gaaggaguac     2160

cuccugaccg aggaggacau cuccaaguuc gacgugcgca agaaguucau cgagcggaac     2220

cugguggaca cgcgcuacgc gagccggguc gugcucaacg cccugcagga gcacuuccgc     2280

gcccacaaga ucgacaccaa gguguccguc gugcgcggcc aguucaccag ccagcugcgg     2340

cgccacuggg gcaucgagaa gacccgggac acguaccacc accacgccgu ggacgcccuc     2400

aucaucgcgg ccagcuccca gcugaaccug uggaagaagc agaagaacac ccucgucagc     2460

uacuccgagg accagcugcu ggacaucgag accggggagc ucaucagcga cgacgaguac     2520

aaggaguccg uguucaaggc ccccuaccag cacuucgugg acacccucaa gagcaaggag     2580

uucgaggacu ccauccuguu cagcuaccag gucgacucca aguucaaccg caagaucagc     2640

gacgccacga ucuacgcgac ccggcaggcc aaggugggca aggacaaggc cgacgagacc     2700

uacgugcucg ggaagaucaa ggacaucuac acccaggacg gcuacgacgc cuucaugaag     2760

aucuacaaga aggacaaguc caaguuccug auguaccgcc acgacccgca gacguucgag     2820

aaggucaucg agcccauccu ggagaacuac cccaacaagc agaucaacga gaagggcaag     2880

gaggugcccu gcaacccguu ccucaaguac aaggaggagc acggguacau ccggaaguac     2940

agcaagaagg gcaacgggcc cgagaucaag ucccugaagu acuacgacag caagcugggc     3000

aaccacaucg acaucacccc caaggacucc aacaacaagg ugguccucca gagcgugucc     3060

cccuggcgcg ccgacgugua cuucaacaag accaccggga aguacgagau ccugggccug     3120

aaguacgcgg accuccaguu cgagaagggc acggggaccu acaagaucag ccaggagaag     3180

uacaacgaca ucaagaagaa ggagggcguc gacagcgacu ccgaguucaa guucacccug     3240

uacaagaacg accugcuccu ggugaaggac accgagacga aggagcagca gcuguuccgg     3300

uuccucagcc gcaccaugcc gaagcagaag cacuacgugg agcugaagcc cuacgacaag     3360

cagaaguucg agggcgggga ggcccugauc aagguccucg ggaacguggc caacuccggc     3420

cagugcaaga agggccuggg caagagcaac aucuccaucu acaaggugcg gaccgacguc     3480

cuggggaacc agcacaucau caagaacgag ggggacaagc ccaagcucga cuucaagcgc     3540

cccgccgcca ccaagaaggc gggccaggcc aagaagaaga aguaaggacu agucccuguu     3600

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

gaauu                                                                 3725


<210>  2504
<211>  4283
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2504
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgacccag      180

uucgagggcu ucaccaaccu guaccaggug agcaagacgc uccgguucga gcugaucccc      240

caggggaaga cccugaagca cauccaggag cagggcuuca ucgaggagga caaggcccgc      300

aacgaccacu acaaggagcu caagcccauc aucgaccgga ucuacaagac cuacgccgac      360

cagugccugc agcuggugca gcucgacugg gagaaccugu ccgccgccau cgacagcuac      420

cgcaaggaga agaccgagga gacgcggaac gcgcugaucg aggagcaggc caccuaccgc      480

aacgccaucc acgacuacuu caucgggcgg accgacaacc ucaccgacgc caucaacaag      540

cgccacgcgg agaucuacaa gggccuguuc aaggccgagc uguucaacgg caagguccuc      600

aagcagcugg ggacggugac caccaccgag cacgagaacg cccugcuccg guccuucgac      660

aaguucacga ccuacuucag cggcuucuac gagaaccgca agaacguguu cuccgccgag      720

gacaucagca ccgccauccc ccaccggauc guccaggaca acuucccgaa guucaaggag      780

aacugccaca ucuucacccg ccugaucacg gcggugcccu cccugcggga gcacuucgag      840

aacgugaaga aggccaucgg gaucuucguc agcaccucca ucgaggaggu guucagcuuc      900

cccuucuaca accagcuccu gacccagacc cagaucgacc uguacaacca gcuccugggc      960

gggaucuccc gcgaggccgg cacggagaag aucaagggcc ugaacgaggu gcucaaccug     1020

gccauccaga agaacgacga gaccgcccac aucaucgcga gccugcccca ccgguucauc     1080

ccccucuuca agcagauccu guccgaccgc aacacccuga gcuucauccu cgaggaguuc     1140

aaguccgacg aggaggucau ccagagcuuc ugcaaguaca agacccugcu gcggaacgag     1200

aacgugcucg agacggccga ggcccuguuc aacgagcuga acuccaucga ccucacccac     1260

aucuucauca gccacaagaa gcuggagacc aucuccagcg cccugugcga ccacugggac     1320

acccuccgca acgcccugua cgagcggcgc aucagcgagc ugacggggaa gaucaccaag     1380

uccgcgaagg agaaggugca gcggagccuc aagcacgagg acaucaaccu gcaggagauc     1440

aucuccgccg ccggcaagga gcugagcgag gccuucaagc agaagaccuc cgagauccuc     1500

agccacgccc acgcggcccu ggaccagccg cugcccacca cgcucaagaa gcaggaggag     1560

aaggagaucc ugaaguccca gcuggacagc cugcucgggc uguaccaccu ccucgacugg     1620

uucgccgucg acgaguccaa cgagguggac cccgaguuca gcgcccgccu gaccggcauc     1680

aagcuggaga uggagccguc ccucagcuuc uacaacaagg cgcgcaacua cgccaccaag     1740

aagcccuacu ccguggagaa guucaagcug aacuuccaga ugcccacccu ggccagcggc     1800

ugggacguca acaaggagaa gaacaacggg gccauccucu ucgugaagaa cggccuguac     1860

uaccugggga ucaugcccaa gcagaagggc cgguacaagg cccucuccuu cgagccgacg     1920

gagaagacca gcgagggguu cgacaagaug uacuacgacu acuuccccga cgcggccaag     1980

augaucccca agugcuccac ccagcugaag gccgugaccg cccacuucca gacccacacg     2040

acccccaucc ugcucagcaa caacuucauc gagccgcugg agaucaccaa ggagaucuac     2100

gaccugaaca accccgagaa ggagcccaag aaguuccaga ccgccuacgc gaagaagacg     2160

ggcgaccaga agggcuaccg cgaggcccuc ugcaagugga ucgacuucac ccgggacuuc     2220

cuguccaagu acaccaagac cacgagcauc gaccugagcu cccuccgccc cagcucccag     2280

uacaaggacc ugggggagua cuacgccgag cugaacccgc uccuguacca caucagcuuc     2340

cagcggaucg ccgagaagga gaucauggac gccgucgaga ccggcaagcu guaccucuuc     2400

cagaucuaca acaaggacuu cgcgaagggg caccacggca agcccaaccu gcacacccug     2460

uacuggaccg gccucuucuc ccccgagaac cuggccaaga cgagcaucaa gcugaacggg     2520

caggccgagc uguucuaccg ccccaagucc cggaugaagc gcauggccca ccggcugggc     2580

gagaagaugc ugaacaagaa gcucaaggac cagaagaccc cgauccccga cacccuguac     2640

caggagcugu acgacuacgu gaaccaccgc cucagccacg accuguccga cgaggcgcgg     2700

gcccugcucc ccaacgugau caccaaggag gucagccacg agaucaucaa ggaccgccgg     2760

uucacguccg acaaguucuu cuuccacgug ccgaucaccc ugaacuacca ggccgccaac     2820

agccccucca aguucaacca gcgcgucaac gccuaccuga aggagcaccc cgagaccccc     2880

aucaucggga ucgaccgggg cgagcgcaac cucaucuaca ucaccgugau cgacagcacg     2940

gggaagaucc uggagcagcg gucccugaac accauccagc aguucgacua ccagaagaag     3000

cucgacaacc gcgagaagga gcggguggcg gcccgccagg ccuggagcgu cgugggcacc     3060

aucaaggacc ugaagcaggg guaccugucc caggugaucc acgagaucgu cgaccucaug     3120

auccacuacc aggccguggu gguccuggag aaccugaacu ucggcuucaa gagcaagcgc     3180

accggcaucg ccgagaaggc gguguaccag caguucgaga agaugcucau cgacaagcug     3240

aacugccugg ugcucaagga cuacccggcc gagaaggucg ggggcgugcu gaaccccuac     3300

cagcugacgg accaguucac cagcuucgcc aagaugggga cccaguccgg cuuccucuuc     3360

uacgugcccg cccccuacac cagcaagauc gacccgcuga cgggcuucgu cgaccccuuc     3420

guguggaaga ccaucaagaa ccacgagucc cggaagcacu uccuggaggg guucgacuuc     3480

cuccacuacg acgugaagac cggcgacuuc auccugcacu ucaagaugaa ccgcaaccug     3540

agcuuccagc gggggcuccc cggcuucaug cccgccuggg acaucgucuu cgagaagaac     3600

gagacccagu ucgacgcgaa ggggacgccg uucaucgccg gcaagcgcau cgugcccgug     3660

aucgagaacc accgguucac cggccgcuac cgggaccugu accccgccaa cgagcugauc     3720

gcccuccugg aggagaaggg gaucgucuuc cgcgacggcu ccaacauccu gcccaagcuc     3780

cuggagaacg acgacagcca cgcgaucgac accauggugg cccugauccg guccgugcuc     3840

cagaugcgca acagcaacgc cgccaccggg gaggacuaca ucaacucccc gguccgggac     3900

cugaacggcg ugugcuucga cagccgcuuc cagaaccccg aguggcccau ggacgccgac     3960

gcgaacgggg ccuaccacau cgcccugaag ggccagcucc ugcugaacca ccucaaggag     4020

uccaaggacc ugaagcugca gaacggcauc agcaaccagg acuggcucgc cuacauccag     4080

gagcugcgga acaagcgccc cgccgcgacg aagaaggccg ggcaggccaa gaagaagaag     4140

uaaggacuag ucccuguucc cagagcccac uuuuuuuucu uuuuuugaaa uaaaauagcc     4200

ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaga auu                                             4283


<210>  2505
<211>  4262
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2505
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaagaa gcgcaaggug ggcauccacg gggucccggc cgcgagcauc      180

uaccaggagu ucgugaacaa guacucccug agcaagaccc uccgguucga gcugaucccc      240

cagggcaaga cccuggagaa caucaaggcc cgcgggcuca uccuggacga cgagaagcgg      300

gccaaggacu acaagaaggc caagcagauc aucgacaagu accaccaguu cuucaucgag      360

gagauccugu ccagcgugug caucuccgag gaccuccugc agaacuacag cgacgucuac      420

uucaagcuga agaaguccga cgacgacaac cuccagaagg acuucaagag cgccaaggac      480

acgaucaaga agcagaucuc cgaguacauc aaggacagcg agaaguucaa gaaccuguuc      540

aaccagaacc ugaucgacgc gaagaagggc caggaguccg accucauccu guggcugaag      600

cagagcaagg acaacgggau cgagcuguuc aaggccaacu ccgacaucac cgacaucgac      660

gaggcccucg agaucaucaa gagcuucaag ggcuggacca ccuacuucaa gggcuuccac      720

gagaaccgca agaacgugua cuccagcaac gacaucccca cguccaucau cuaccggauc      780

guggacgaca accugcccaa guuccucgag aacaaggcca aguacgagag ccugaaggac      840

aaggcgccgg aggccaucaa cuacgagcag aucaagaagg accuggccga ggagcucacc      900

uucgacaucg acuacaagac cuccgagguc aaccagcgcg uguucagccu ggacgaggug      960

uucgagaucg ccaacuucaa caacuaccug aaccagagcg ggaucaccaa guucaacacg     1020

aucaucggcg ggaaguucgu caacggcgag aacaccaagc ggaaggggau caacgaguac     1080

aucaaccucu acucccagca gaucaacgac aagacccuga agaaguacaa gaugagcgug     1140

cuguucaagc agauccucuc cgacaccgag agcaaguccu ucgugaucga caagcuggag     1200

gacgacagcg acgucgugac gaccaugcag uccuucuacg agcagaucgc cgcguucaag     1260

accguggagg agaaguccau caaggagacc cugucccucc uguucgacga ccugaaggcc     1320

cagaagcucg accugagcaa gaucuacuuc aagaacgaca agucccugac ggaccucagc     1380

cagcaggucu ucgacgacua cuccgugauc ggcaccgccg ugcuggagua caucacccag     1440

cagaucgccc ccaagaaccu ggacaacccc agcaagaagg agcaggagcu caucgccaag     1500

aagaccgaga aggcgaagua ccugucccug gagacgauca agcucgcccu ggaggaguuc     1560

aacaagcacc gcgacaucga caagcagugc cgguucgagg agauccuggc caacuucgcc     1620

gccaucccca ugaucuucga cgagaucgcg cagaacaagg acaaccucgc ccagaucagc     1680

aucaaguacc agaaccaggg caagaaggac cugcugcagg ccuccgccga ggacgacguc     1740

aaggccauca aggaccuccu ggaccagacc aacaaccugc ugcacaagcu caagaucuuc     1800

cacaucagcc aguccgagga caaggcgaac auccuggaca aggacgagca cuucuaccug     1860

guguucgagg agugcuacuu cgagcucgcc aacaucgugc cgcuguacaa caagauccgc     1920

aacuacauca cccagaagcc cuacagcgac gagaaguuca agcugaacuu cgagaacagc     1980

acccucgcca acggguggga caagaacaag gagcccgaca acacggccau ccuguucauc     2040

aaggacgaca aguacuaccu gggcgucaug aacaagaaga acaacaagau cuucgacgac     2100

aaggcgauca aggagaacaa gggggagggc uacaagaaga ucguguacaa gcuccugccc     2160

ggcgccaaca agaugcugcc gaagguguuc uucuccgcca agagcaucaa guucuacaac     2220

cccuccgagg acauccuccg gauccgcaac cacagcaccc acaccaagaa cgggagcccc     2280

cagaagggcu acgagaaguu cgaguucaac aucgaggacu gccggaaguu caucgacuuc     2340

uacaagcaga gcaucuccaa gcaccccgag uggaaggacu ucggguuccg cuucagcgac     2400

acccagcggu acaacuccau cgacgaguuc uaccgcgagg ucgagaacca gggcuacaag     2460

cugacguucg agaacaucag cgaguccuac aucgacagcg uggucaacca ggggaagcug     2520

uaccucuucc agaucuacaa caaggacuuc uccgccuaca gcaagggccg gccgaaccug     2580

cacacccugu acuggaaggc ccucuucgac gagcgcaacc ugcaggacgu gguguacaag     2640

cugaacggcg aggcggagcu guucuaccgg aagcagucca uccccaagaa gaucacccac     2700

cccgccaagg aggccaucgc caacaagaac aaggacaacc ccaagaagga gagcgucuuc     2760

gaguacgacc ucaucaagga caagcgcuuc accgaggaca aguucuucuu ccacugcccg     2820

aucacgauca acuucaaguc cagcggggcc aacaaguuca acgacgagau caaccugcuc     2880

cugaaggaga aggcgaacga cgugcacauc cugagcaucg accggggcga gcgccaccuc     2940

gccuacuaca cccuggugga cgggaagggc aacaucauca agcaggacac cuucaacauc     3000

aucgggaacg accgcaugaa gaccaacuac cacgacaagc uggccgccau cgagaaggac     3060

cgggacuccg cccgcaagga cuggaagaag aucaacaaca ucaaggagau gaaggagggc     3120

uaccucagcc aggucgugca cgagaucgcg aagcugguga ucgaguacaa cgccaucguc     3180

guguucgagg accugaacuu cggcuucaag cgggggcgcu ucaaggugga gaagcagguc     3240

uaccagaagc ucgagaagau gcugaucgag aagcugaacu accucguguu caaggacaac     3300

gaguucgaca agacgggcgg ggugcugcgg gccuaccagc ugaccgcccc cuucgagacc     3360

uucaagaaga ugggcaagca gaccgggauc aucuacuacg uccccgcggg cuucaccucc     3420

aagaucugcc ccgugacggg cuucgugaac cagcucuacc cgaaguacga gagcgucucc     3480

aagagccagg aguucuucuc caaguucgac aagaucugcu acaaccugga caagggguac     3540

uucgaguuca gcuucgacua caagaacuuc ggcgacaagg ccgccaaggg gaaguggacc     3600

aucgccuccu ucggcagccg ccugaucaac uuccggaacu ccgacaagaa ccacaacugg     3660

gacacccgcg agguguaccc caccaaggag cucgagaagc ugcugaagga cuacagcauc     3720

gaguacggcc acggggagug caucaaggcc gcgaucugcg gcgaguccga caagaaguuc     3780

uucgccaagc ucacgagcgu gcugaacacc auccugcaga ugcggaacuc caagaccggg     3840

accgagcucg acuaccugau cagccccguc gccgacguga acggcaacuu cuucgacucc     3900

cgccaggccc ccaagaacau gccgcaggac gccgacgcga acggggccua ccacaucggc     3960

cugaagggcc ucaugcugcu ggggcggauc aagaacaacc aggagggcaa gaagcucaac     4020

cuggugauca agaacgagga guacuucgag uucguccaga accgcaacaa caagcggccc     4080

gccgccacga agaaggccgg gcaggcgaag aagaagaagu aaggacuagu cccuguuccc     4140

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     4260

uu                                                                    4262


<210>  2506
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2506
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcggac aagaaguaca gcaucggccu ggacaucggg      180

accaacuccg ugggcugggc cgugaucacc gacgaguaca agguccccag caagaaguuc      240

aaggugcugg ggaacacgga ccggcacucc aucaagaaga accugaucgg cgcccugcug      300

uucgacagcg gcgagaccgc cgaggccacc cgccugaagc ggaccgcgcg ccggcgcuac      360

acgcggcgca agaaccggau cugcuaccug caggagaucu ucuccaacga gauggccaag      420

guggacgaca gcuucuucca ccgccucgag gaguccuucc uggucgagga ggacaagaag      480

cacgagcggc accccaucuu cgggaacauc guggacgagg uggccuacca cgagaaguac      540

cccaccaucu accaccuccg caagaagcuc gucgacagca ccgacaaggc cgaccugcgg      600

cugaucuacc ucgcgcuggc ccacaugauc aaguuccgcg gccacuuccu gaucgagggg      660

gaccucaacc cggacaacuc cgacguggac aagcuguuca uccagcuggu gcagaccuac      720

aaccagcugu ucgaggagaa ccccaucaac gccagcggcg ucgacgccaa ggccauccug      780

uccgcgcggc ugagcaaguc ccgccggcuc gagaaccuga ucgcccagcu gcccggggag      840

aagaagaacg gccucuucgg caaccugauc gcccugagcc ucgggcugac gcccaacuuc      900

aaguccaacu ucgaccuggc cgaggacgcc aagcuccagc ugagcaagga caccuacgac      960

gacgaccugg acaaccuccu ggcgcagauc ggcgaccagu acgccgaccu guuccucgcc     1020

gccaagaacc uguccgacgc cauccugcuc agcgacaucc ugcgcgugaa caccgagauc     1080

accaaggcgc cgcuguccgc cagcaugauc aagcgguacg acgagcacca ccaggaccuc     1140

acgcugcuga aggcccucgu gcgccagcag cugcccgaga aguacaagga gaucuucuuc     1200

gaccagagca agaacgggua cgccggcuac aucgacggcg gggccuccca ggaggaguuc     1260

uacaaguuca ucaagcccau ccuggagaag auggacggca ccgaggagcu ccuggucaag     1320

cugaaccggg aggaccugcu ccgcaagcag cgcaccuucg acaacgggag caucccccac     1380

cagauccacc ugggcgagcu gcacgcgauc cuccggcgcc aggaggacuu cuacccguuc     1440

cugaaggaca accgggagaa gaucgagaag auccugaccu uccgcauccc cuacuacgug     1500

gggccccucg cccggggcaa cucccgcuuc gccuggauga cgcgcaagag cgaggagacc     1560

aucacccccu ggaacuucga ggaggugguc gacaagggcg ccuccgcgca gagcuucauc     1620

gagcggauga ccaacuucga caagaaccug ccgaacgaga aggugcugcc caagcacucc     1680

cuccuguacg aguacuucac gguguacaac gagcugacca aggucaagua cgugaccgag     1740

gggaugcgga agcccgccuu ccucagcggc gagcagaaga aggccaucgu ggaccugcug     1800

uucaagacca accgcaaggu cacggugaag cagcucaagg aggacuacuu caagaagauc     1860

gagugcuucg acuccgucga gaucagcggg guggaggacc gguucaacgc cucccugggc     1920

accuaccacg accugcucaa gaucaucaag gacaaggacu uccuggacaa cgaggagaac     1980

gaggacaucc uggaggacau cgugcucacc cugacccugu ucgaggaccg cgagaugauc     2040

gaggagcggc ucaagacgua cgcccaccug uucgacgaca aggucaugaa gcagcugaag     2100

cgccggcgcu acaccgggug gggccggcuc agccgcaagc ugaucaacgg cauccgggac     2160

aagcaguccg ggaagaccau ccuggacuuc cucaagagcg acggcuucgc gaaccgcaac     2220

uucaugcagc ugauccacga cgacucccug accuucaagg aggacaucca gaaggcccag     2280

gugagcgggc agggcgacuc ccuccacgag cacaucgcca accuggccgg gagccccgcc     2340

aucaagaagg gcauccugca gacggugaag gucguggacg agcucgugaa ggucaugggc     2400

cgccacaagc cggagaacau cgugaucgag auggcgcggg agaaccagac cacccagaag     2460

gggcagaaga acucccgcga gcggaugaag cgcaucgagg agggcaucaa ggagcugggg     2520

agccagaucc ugaaggagca ccccguggag aacacccagc uccagaacga gaagcuguac     2580

cuguacuacc uccagaacgg ccgggacaug uacgucgacc aggagcugga caucaaccgc     2640

cugagcgacu acgacgugga ccacaucgug ccccaguccu uccucaagga cgacagcauc     2700

gacaacaagg uccugacgcg guccgacaag aaccgcggca agagcgacaa cgugcccucc     2760

gaggaggugg ucaagaagau gaagaacuac uggcggcagc ugcucaacgc caagcugauc     2820

acccagcgca aguucgacaa ccugaccaag gccgagcgcg ggggccucag cgagcuggac     2880

aaggccgggu ucaucaagcg gcagcuggug gagacccggc agaucaccaa gcacguggcc     2940

cagauccucg acucccgcau gaacacgaag uacgacgaga acgacaagcu gauccgggag     3000

gucaagguga ucacccucaa gagcaagcuc guguccgacu uccgcaagga cuuccaguuc     3060

uacaaggucc gggagaucaa caacuaccac cacgcgcacg acgccuaccu gaacgccgug     3120

gugggcaccg cccugaucaa gaaguacccg aagcucgaga gcgaguucgu cuacggggac     3180

uacaaggugu acgacgugcg caagaugauc gcgaaguccg agcaggagau cggcaaggcc     3240

accgccaagu acuucuucua cagcaacauc augaacuucu ucaagacgga gaucacccug     3300

gccaacggcg agauccggaa gcgcccccug aucgagacca acggggagac cggcgagauc     3360

gucugggaca aggggcggga cuucgccacg gugcgcaagg ugcucuccau gccccagguc     3420

aacaucguga agaagaccga ggugcagacc ggcggguuca gcaaggaguc cauccugccc     3480

aagcgcaaca gcgacaagcu gaucgcgcgg aagaaggacu gggacccgaa gaaguacggc     3540

ggcuucgacu cccccaccgu cgccuacagc gugcucgugg ucgccaaggu ggagaagggc     3600

aagagcaaga agcugaaguc cgugaaggag cugcucggga ucacgaucau ggagcgcagc     3660

uccuucgaga agaaccccau cgacuuccug gaggccaagg gguacaagga ggucaagaag     3720

gaccugauca ucaagcuccc gaaguacagc cuguucgagc uggagaacgg ccggaagcgc     3780

augcucgccu ccgcggggga gcugcagaag ggcaacgagc uggcccuccc cagcaaguac     3840

gugaacuucc uguaccuggc cucccacuac gagaagcuca agggcagccc cgaggacaac     3900

gagcagaagc agcuguucgu ggagcagcac aagcacuacc uggacgagau caucgagcag     3960

aucuccgagu ucagcaagcg ggucauccuc gccgacgcca accuggacaa ggugcugucc     4020

gcguacaaca agcaccgcga caagcccauc cgggagcagg ccgagaacau cauccaccuc     4080

uucacccuga ccaaccuggg ggccccggcc gcguucaagu acuucgacac cacgaucgac     4140

cgcaagcggu acaccagcac caaggaggug cucgacgcca cccugaucca ccaguccauc     4200

acgggccugu acgagacccg caucgaccuc agccagcucg ggggcgacaa gcggcccgcc     4260

gccaccaaga aggccgggca ggcgaagaag aagaaguaag gacuaguguc caccuguccc     4320

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     4380

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaagaa uu                                                         4452


<210>  2507
<211>  5235
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2507
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaac uucaagaucc ugcccaucgc caucgaccuc      180

ggcgugaaga acaccggggu guucagcgcc uucuaccaga agggcaccuc ccuggagcgg      240

cuggacaaca agaacgggaa ggucuacgag cucagcaagg acuccuacac gcugcugaug      300

aacaaccgca ccgcccggcg ccaccagcgg cgcggcaucg accggaagca gcucgugaag      360

cgccuguuca agcugaucug gaccgagcag cucaaccugg agugggacaa ggacacccag      420

caggccauca gcuuccuguu caaccggcgc ggcuucuccu ucaucacgga cggguacagc      480

cccgaguacc ucaacaucgu gcccgagcag gucaaggcga uccugaugga caucuucgac      540

gacuacaacg gcgaggacga ccuggacucc uaccucaagc uggccaccga gcaggagagc      600

aagaucuccg agaucuacaa caagcugaug cagaagaucc ucgaguucaa gcugaugaag      660

cugugcaccg acaucaagga cgacaaggug agcaccaaga cgcucaagga gaucaccucc      720

uacgaguucg agcugcuggc cgacuaccuc gccaacuaca gcgagucccu gaagacccag      780

aaguucagcu acaccgacaa gcaggggaac cugaaggagc ucuccuacua ccaccacgac      840

aaguacaaca uccaggaguu ccugaagcgg cacgcgacga ucaacgaccg cauccuggac      900

acccuccuga ccgacgaccu ggacaucugg aacuucaacu ucgagaaguu cgacuucgac      960

aagaacgagg agaagcucca gaaccaggag gacaaggacc acauccaggc ccaccuccac     1020

cacuucgugu ucgccgucaa caagaucaag agcgagaugg ccuccggcgg gcggcaccgc     1080

agccaguacu uccaggagau caccaacgug cuggacgaga acaaccacca ggagggcuac     1140

cugaagaacu ucugcgagaa ccugcacaac aagaaguacu ccaaccugag cgugaagaac     1200

cucgucaacc ugaucggcaa ccugagcaac cucgagcuga agccgcugcg gaaguacuuc     1260

aacgacaaga uccacgccaa ggcggaccac ugggacgagc agaaguucac ggagaccuac     1320

ugccacugga uccucgggga guggcgcgug ggcgugaagg accaggacaa gaaggacggg     1380

gccaaguacu ccuacaagga ccugugcaac gagcugaagc agaaggucac caaggccggc     1440

cucguggacu uccugcugga gcuggacccc ugccggacca ucccgcccua ccucgacaac     1500

aacaaccgca agccgcccaa gugccagagc cugauccuga accccaaguu ccucgacaac     1560

caguacccca acuggcagca guaccugcag gagcugaaga agcuccaguc cauccagaac     1620

uaccuggaca gcuucgagac ggaccugaag gugcucaagu ccagcaagga ccagcccuac     1680

uucgucgagu acaaguccag caaccagcag aucgccuccg gccagcggga cuacaaggac     1740

cuggacgccc gcauccugca guucaucuuc gaccggguga aggcgagcga cgagcuccug     1800

cugaacgaga ucuacuucca ggccaagaag cucaagcaga aggccuccag cgagcuggag     1860

aagcuggagu ccagcaagaa gcucgacgag gugaucgcca acucccagcu gagccagauc     1920

cugaaguccc agcacaccaa cgggaucuuc gagcagggca ccuuccucca ccuggucugc     1980

aaguacuaca agcagcgcca gcgcgcccgg gacagccgcc uguacaucau gcccgaguac     2040

cgguacgaca agaagcucca caaguacaac aacaccgggc gcuucgacga cgacaaccag     2100

cugcugacgu acugcaacca caagccccgg cagaagcgcu accagcuccu gaacgaccug     2160

gcgggcgugc uccagguguc ccccaacuuc cugaaggaca agaucgggag cgacgacgac     2220

cuguucauca gcaaguggcu cgucgagcac auccggggcu ucaagaaggc cugcgaggac     2280

ucccugaaga uccagaagga caaccgcggc cugcucaacc acaagaucaa caucgcccgg     2340

aacaccaagg ggaagugcga gaaggagauc uucaaccuga ucugcaagau cgagggcagc     2400

gaggacaaga aggggaacua caagcacggc cuggccuacg agcucggggu gcugcuguuc     2460

ggcgagccga acgaggccuc caagcccgag uucgaccgca agaucaagaa guucaacagc     2520

aucuacuccu ucgcgcagau ccagcagauc gccuucgccg agcggaaggg caacgccaac     2580

acgugcgcgg ucugcagcgc cgacaacgcc caccgcaugc agcagaucaa gaucaccgag     2640

cccguggagg acaacaagga caagaucauc cucuccgcca aggcccagcg gcugcccgcg     2700

aucccgaccc gcaucgugga cggggccguc aagaagaugg ccaccauccu ggccaagaac     2760

aucguggacg acaacuggca gaacaucaag cagguccuca gcgccaagca ccagcugcac     2820

auccccauca ucaccgaguc caacgcguuc gaguucgagc ccgcccuggc cgacgugaag     2880

ggcaagagcc ucaaggaccg gcgcaagaag gcccuggagc ggaucucccc cgagaacauc     2940

uucaaggaca agaacaaccg caucaaggag uucgccaagg ggaucagcgc guacuccggc     3000

gccaaccuga ccgacgggga cuucgacggc gccaaggagg agcucgacca caucaucccg     3060

cggagccaca agaaguacgg cacgcugaac gacgaggcca accugaucug cgugacccgc     3120

ggggacaaca agaacaaggg caaccgcauc uucugccucc gggaccuggc ggacaacuac     3180

aagcugaagc aguucgagac caccgacgac cucgagaucg agaagaagau cgccgacacg     3240

aucugggacg ccaacaagaa ggacuucaag uucgggaacu accgcuccuu caucaaccug     3300

acgccccagg agcagaaggc cuuccggcac gcccuguucc ucgcggacga gaaccccauc     3360

aagcaggccg ugauccgcgc caucaacaac cggaaccgca ccuucgucaa cggcacccag     3420

cgguacuucg ccgaggugcu ggccaacaac aucuaccugc gcgcgaagaa ggagaaccuc     3480

aacaccgaca agaucagcuu cgacuacuuc ggcaucccca ccaucgggaa cggccggggg     3540

aucgccgaga uccgccagcu guacgagaag guggacuccg acauccaggc cuacgccaag     3600

ggcgacaagc cgcaggccag cuacagccac cugaucgacg cgaugcucgc cuucugcauc     3660

gccgccgacg agcaccggaa cgacgggucc aucggccugg agaucgacaa gaacuacagc     3720

cuguaccccc ucgacaagaa caccggcgag gucuucacca aggacaucuu cucccagauc     3780

aagaucaccg acaacgaguu cagcgacaag aagcuggugc gcaagaaggc gaucgagggg     3840

uucaacacgc accggcagau gacccgcgac ggcaucuacg ccgagaacua ccugcccauc     3900

cucauccaca aggagcugaa cgaggugcgg aagggguaca ccuggaagaa cuccgaggag     3960

aucaagaucu ucaagggcaa gaaguacgac auccagcagc ugaacaaccu cgucuacugc     4020

cugaaguucg uggacaagcc gaucagcauc gacauccaga ucuccacccu ggaggagcuc     4080

cgcaacaucc ugacgaccaa caacaucgcc gccaccgccg aguacuacua caucaaccug     4140

aagacccaga agcuccacga guacuacauc gagaacuaca acacggcgcu gggguacaag     4200

aaguacagca aggagaugga guuccugcgg ucccucgccu accgcagcga gcgggugaag     4260

aucaagucca ucgacgacgu caagcagguc cuggacaagg acagcaacuu caucaucggc     4320

aagaucaccc ugcccuucaa gaaggagugg cagcgccucu accgggagug gcagaacacc     4380

accaucaagg acgacuacga guuccugaag uccuucuuca acgucaagag caucacgaag     4440

cugcacaaga aggugcgcaa ggacuucucc cuccccauca gcaccaacga gggcaaguuc     4500

cuggugaagc gcaagaccug ggacaacaac uucaucuacc agauccugaa cgacuccgac     4560

agccgggccg acgggaccaa gcccuucauc ccggccuucg acaucuccaa gaacgagauc     4620

guggaggcca ucaucgacag cuucacgagc aagaacaucu ucuggcuccc caagaacauc     4680

gagcugcaga agguggacaa caagaacauc uucgcgaucg acaccuccaa gugguucgag     4740

guggagaccc ccagcgaccu gcgcgacauc ggcaucgcca ccauccagua caagaucgac     4800

aacaacuccc ggcccaaggu gcgcgucaag cuggacuacg ugaucgacga cgacagcaag     4860

aucaacuacu ucaugaacca cucccuccuc aagagccggu acccggacaa ggugcuggag     4920

auccugaagc aguccacgau caucgaguuc gagagcuccg gguucaacaa gaccaucaag     4980

gagaugcugg gcaugaagcu ggccgggauc uacaacgaga ccagcaacaa caagcgcccc     5040

gccgccacca agaaggcggg ccaggccaag aagaagaagu aaggacuagu guccaccugu     5100

cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac     5160

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5220

aaaaaaaaaa gaauu                                                      5235


<210>  2508
<211>  3507
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2508
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaag cggaacuaca uccugggccu cgacaucggg      180

aucaccagcg ugggcuacgg gaucaucgac uacgagaccc gcgacgugau cgacgccggc      240

guccggcugu ucaaggaggc caacguggag aacaacgagg gccgccgguc caagcgcggg      300

gcccggcgcc ugaagcggcg ccggcgccac cggauccagc gcgugaagaa gcuccuguuc      360

gacuacaacc ugcucacgga ccacagcgag cuguccggca ucaaccccua cgaggcccgg      420

gucaaggggc ugagccagaa gcucuccgag gaggaguuca gcgcggcccu gcugcaccuc      480

gccaagcgcc ggggcgugca caacgugaac gaggucgagg aggacaccgg gaacgagcug      540

uccaccaagg agcagaucag ccgcaacucc aaggcccugg aggagaagua cguggcggag      600

cuccagcugg agcggcugaa gaaggacggc gaggugcgcg gcagcaucaa ccgguucaag      660

accuccgacu acgucaagga ggccaagcag cuccugaagg ugcagaaggc cuaccaccag      720

cuggaccaga gcuucaucga cacguacauc gaccuccugg agacccgccg caccuacuac      780

gaggggcccg gcgagggguc ccccuucggc uggaaggaca ucaaggagug guacgagaug      840

cugaugggcc acugcaccua cuucccggag gagcuccgga gcgugaagua cgccuacaac      900

gccgaccugu acaacgcgcu gaacgaccuc aacaaccugg ucaucacgcg cgacgagaac      960

gagaagcugg aguacuacga gaaguuccag aucaucgaga acguguucaa gcagaagaag     1020

aagcccaccc ucaagcagau cgccaaggag auccugguga acgaggagga caucaagggg     1080

uaccggguca ccuccaccgg caagcccgag uucacgaacc ugaaggugua ccacgacauc     1140

aaggacauca ccgcccgcaa ggagaucauc gagaacgccg agcuccugga ccagaucgcc     1200

aagauccuga ccaucuacca gagcuccgag gacauccagg aggagcucac caaccugaac     1260

agcgagcuga cgcaggagga gaucgagcag aucuccaacc ucaaggggua caccggcacc     1320

cacaaccuga gccugaaggc gaucaaccuc auccuggacg agcuguggca caccaacgac     1380

aaccagaucg ccaucuucaa ccggcucaag cuggugccca agaaggucga ccugagccag     1440

cagaaggaga ucccgacgac ccucguggac gacuucaucc ugucccccgu ggucaagcgc     1500

agcuucaucc aguccaucaa ggugaucaac gccaucauca agaaguacgg gcugcccaac     1560

gacaucauca ucgagcuggc ccgggagaag aacagcaagg acgcccagaa gaugaucaac     1620

gagaugcaga agcgcaaccg gcagaccaac gagcgcaucg aggagaucau ccggaccacg     1680

ggcaaggaga acgcgaagua ccucaucgag aagaucaagc ugcacgacau gcaggagggc     1740

aagugccugu acucccucga ggccaucccc cuggaggacc ugcucaacaa cccguucaac     1800

uacgaggucg accacaucau cccccgcagc guguccuucg acaacagcuu caacaacaag     1860

gugcugguca agcaggagga gaacuccaag aaggggaacc ggacccccuu ccaguaccug     1920

agcuccagcg acuccaagau cagcuacgag accuucaaga agcacauccu caaccuggcc     1980

aagggcaagg ggcgcaucuc caagaccaag aaggaguacc ugcucgagga gcgggacauc     2040

aaccgcuuca gcgugcagaa ggacuucauc aaccggaacc ugguggacac gcgcuacgcc     2100

acccggggcc ugaugaaccu ccugcgcucc uacuuccgcg ucaacaaccu ggacgugaag     2160

gucaagagca ucaacggggg cuucaccucc uuccuccggc gcaaguggaa guucaagaag     2220

gagcggaaca agggcuacaa gcaccacgcc gaggacgcgc ugaucaucgc caacgccgac     2280

uucaucuuca aggaguggaa gaagcuggac aaggccaaga aggugaugga gaaccagaug     2340

uucgaggaga agcaggcgga gagcaugccc gagaucgaga ccgagcagga guacaaggag     2400

aucuucauca cgccgcacca gaucaagcac aucaaggacu ucaaggacua caaguacagc     2460

caccgcgugg acaagaagcc caaccgggag cucaucaacg acacccugua cuccacccgc     2520

aaggacgaca aggggaacac ccugaucgug aacaaccuca acggccugua cgacaaggac     2580

aacgacaagc ugaagaagcu caucaacaag agccccgaga agcugcugau guaccaccac     2640

gacccccaga cguaccagaa gcucaagcug aucauggagc aguacgggga cgagaagaac     2700

ccgcuguaca aguacuacga ggagaccggc aacuaccuca ccaaguacuc caagaaggac     2760

aacgggcccg ucaucaagaa gaucaaguac uacggcaaca agcugaacgc ccaccuggac     2820

aucaccgacg acuaccccaa cagccggaac aaggugguga agcucucccu gaagcccuac     2880

cgcuucgacg ucuaccugga caacggcgug uacaaguucg ugaccgucaa gaaccucgac     2940

gugaucaaga aggagaacua cuacgaggug aacagcaagu gcuacgagga ggccaagaag     3000

cugaagaaga ucuccaacca ggccgaguuc aucgccagcu ucuacaacaa cgaccugauc     3060

aagaucaacg gggagcucua ccgggucauc ggcgugaaca acgaccugcu gaaccgcauc     3120

gaggugaaca ugaucgacau cacguaccgg gaguaccucg agaacaugaa cgacaagcgc     3180

ccgccccgga ucaucaagac caucgcgucc aagacccaga gcaucaagaa guacuccacc     3240

gacauccugg ggaaccugua cgaggucaag agcaagaagc acccccagau caucaagaag     3300

ggcaagcgcc ccgccgccac gaagaaggcc ggccaggcca agaagaagaa guaaggacua     3360

guguccaccu gucccuccug ggcugcugga uugucucguu uuccugccaa auaaacagga     3420

ucagcgcuuu acagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aagaauu                                         3507


<210>  2509
<211>  4575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2509
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgcug uucaacaagu gcaucaucau cagcaucaac      180

cucgacuucu ccaacaagga gaagugcaug accaagcccu acagcaucgg ccuggacauc      240

gggaccaacu ccgugggcug ggccgugauc acggacaacu acaagguccc cagcaagaag      300

augaaggugc uggggaacac cuccaagaag uacaucaaga agaaccuccu gggcgugcug      360

cuguucgaca gcggcaucac cgccgagggg cggcgccuga agcggaccgc ccgccggcgc      420

uacacgcggc gccggaaccg cauccuguac cuccaggaga ucuucuccac cgagauggcc      480

acccuggacg acgcguucuu ccagcggcug gacgacagcu uccucguccc cgacgacaag      540

cgcgacucca aguacccgau cuucggcaac cugguggagg agaaggugua ccacgacgag      600

uuccccacca ucuaccaccu ccggaaguac cucgccgaca gcacgaagaa ggccgaccug      660

cgccuggucu accucgcccu ggcgcacaug aucaaguacc gggggcacuu ccugaucgag      720

ggcgaguuca acuccaagaa caacgacauc cagaagaacu uccaggacuu ccucgacacc      780

uacaacgcca ucuucgagag cgaccugucc cuggagaaca gcaagcagcu cgaggagauc      840

gugaaggaca agaucuccaa gcuggagaag aaggaccgca uccugaagcu guuccccggg      900

gagaagaaca gcggcaucuu cuccgaguuc cugaagcuga ucgugggcaa ccaggccgac      960

uuccggaagu gcuucaaccu cgacgagaag gccagccugc acuucuccaa ggagagcuac     1020

gacgaggacc uggagacccu ccugggguac aucggcgacg acuacagcga cgucuuccug     1080

aaggccaaga agcucuacga cgcgauccug cuguccgggu uccucaccgu gacggacaac     1140

gagaccgagg cgccccugag cuccgccaug aucaagcgcu acaacgagca caaggaggac     1200

cuggcccucc ugaaggagua cauccggaac aucagccuga agaccuacaa cgagguguuc     1260

aaggacgaca ccaagaacgg cuacgccggc uacaucgacg ggaagacgaa ccaggaggac     1320

uucuacgucu accucaagaa ccugcuggcc gaguucgagg gcgccgacua cuuccucgag     1380

aagaucgacc gcgaggacuu ccugcggaag cagcgcaccu ucgacaacgg guccaucccg     1440

uaccagaucc accuccagga gaugcgcgcc auccuggaca agcaggccaa guucuacccc     1500

uuccucgcca agaacaagga gcggaucgag aagauccuga ccuuccgcau ccccuacuac     1560

gugggccccc uggcgcgggg gaacagcgac uucgccuggu ccauccgcaa gcggaacgag     1620

aagaucaccc cguggaacuu cgaggacgug aucgacaagg agagcuccgc cgaggccuuc     1680

aucaaccgca ugacgagcuu cgaccucuac cugcccgagg agaagguccu gcccaagcac     1740

ucccuccugu acgagaccuu caacguguac aacgagcuga ccaaggugcg guucaucgcc     1800

gagagcaugc gcgacuacca guuccucgac uccaagcaga agaaggacau cguccggcug     1860

uacuucaagg acaagcgcaa ggugaccgac aaggacauca ucgaguaccu gcacgcgauc     1920

uacggcuacg acggcaucga gcucaagggg aucgagaagc aguucaacag cucccugagc     1980

acguaccacg accugcucaa caucaucaac gacaaggagu uccuggacga cuccagcaac     2040

gaggccauca ucgaggagau cauccacacc cugaccaucu ucgaggaccg ggagaugauc     2100

aagcagcgcc ucuccaaguu cgagaacauc uucgacaaga gcgugcugaa gaagcugagc     2160

cggcgccacu acaccggcug ggggaagcuc uccgccaagc ugaucaacgg cauccgggac     2220

gagaaguccg ggaacacgau ccuggacuac cucaucgacg acggcaucag caaccgcaac     2280

uucaugcagc ugauccacga cgacgcccug agcuucaaga agaagaucca gaaggcgcag     2340

aucaucggcg acgaggacaa ggggaacauc aaggaggucg ugaagucccu ccccggcagc     2400

ccggccauca agaaggggau ccugcagucc aucaagaucg ucgacgagcu ggugaaggug     2460

augggcgggc ggaagcccga gagcaucguc guggagaugg cccgcgagaa ccaguacacc     2520

aaccagggca aguccaacag ccagcagcgg cucaagcgcc uggagaaguc ccugaaggag     2580

cucggcagca agauccugaa ggagaacauc cccgccaagc uguccaagau cgacaacaac     2640

gcccuccaga acgaccgccu guaccuguac uaccuccaga acgggaagga cauguacacc     2700

ggcgacgacc uggacaucga ccggcugagc aacuacgaca ucgaccacau caucccccag     2760

gcguuccuca aggacaacuc caucgacaac aaggugcugg ucagcuccgc cagcaaccgc     2820

gggaaguccg acgacuuccc gagccuggag guggugaaga agcggaagac cuucugguac     2880

cagcuccuca agagcaagcu gaucucccag cgcaaguucg acaaccugac gaaggccgag     2940

cggggcggcc ugcugcccga ggacaaggcc ggguucaucc agcgccagcu cgucgagacc     3000

cggcagauca ccaagcacgu ggcccgccug cuggacgaga aguucaacaa caagaaggac     3060

gagaacaacc gggcggugcg caccgucaag aucaucacgc ucaagagcac ccuggugucc     3120

caguuccgga aggacuucga gcuguacaag gugcgcgaga ucaacgacuu ccaccacgcc     3180

cacgacgccu accucaacgc cgucaucgcc agcgcgcugc ugaagaagua ccccaagcuc     3240

gagcccgagu ucguguacgg cgacuacccg aaguacaacu ccuuccggga gcgcaagagc     3300

gccaccgaga agguguacuu cuacuccaac aucaugaaca ucuucaagaa guccaucagc     3360

cuggccgacg ggcgggucau cgagcgcccc cugaucgagg ugaacgagga gaccggcgag     3420

agcgugugga acaaggaguc cgaccucgcc accguccggc gcgugcugag cuacccccag     3480

gugaacgucg ugaagaaggu ggaggagcag aaccacgggc uggaccgggg caagcccaag     3540

ggccucuuca acgcgaaccu guccagcaag ccgaagccca acuccaacga gaaccugguc     3600

ggggccaagg aguaccucga ccccaagaag uacggcgggu acgccggcau cagcaacucc     3660

uucgccgugc uggugaaggg gacgaucgag aagggcgcca agaagaagau caccaacguc     3720

cuggaguucc agggcaucag cauccucgac cgcaucaacu accggaagga caagcugaac     3780

uuccugcucg agaaggggua caaggacauc gagcugauca ucgagcugcc gaaguacagc     3840

cucuucgagc uguccgacgg cagccgccgc augcuggcgu ccauccucag caccaacaac     3900

aagcgcgggg agauccacaa gggcaaccag aucuuccugu cccagaaguu cgugaagcug     3960

cucuaccacg ccaagcggau cagcaacacc aucaacgaga accaccggaa guacguggag     4020

aaccacaaga aggaguucga ggagcuguuc uacuacaucc uggaguucaa cgagaacuac     4080

gucggggcca agaagaacgg caagcuccug aacuccgccu uccagagcug gcagaaccac     4140

uccaucgacg agcugugcag cuccuucauc ggccccacgg ggagcgagcg caagggccuc     4200

uucgagcuga ccucccgggg gagcgccgcg gacuucgagu uccugggcgu gaagaucccc     4260

cgcuaccggg acuacacccc cuccagccuc cugaaggacg ccacccugau ccaccagucc     4320

gugacggggc uguacgagac ccgcaucgac cucgccaagc ugggcgaggg caagcggccg     4380

gccgccacca agaaggcggg gcaggccaag aagaagaagu aaggacuagu guccaccugu     4440

cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac     4500

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa gaauu                                                      4575


<210>  2510
<211>  3651
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2510
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgugg uacgccagcc ugauguccgc ccaccaccuc      180

cgggugggca ucgacguggg gacccacagc gucggccugg ccacccugcg cguggacgac      240

cacgggacgc ccaucgagcu ccuguccgcc cugagccaca uccacgacuc cggcgugggc      300

aaggagggga agaaggacca cgacacccgg aagaagcuca gcggcaucgc gcgccgggcc      360

cgccggcugc ugcaccaccg ccggacccag cuccagcagc uggacgaggu ccugcgcgac      420

cucggguucc ccauccccac cccgggcgag uuccuggacc ugaacgagca gacggacccc      480

uaccgggugu ggcgcgugcg ggcccgccuc gucgaggaga agcugcccga ggagcugcgg      540

gggcccgcca ucuccauggc ggugcgccac aucgcccggc accgcggcug gcggaacccg      600

uacagcaagg uggagucccu ccugagcccc gccgaggagu cccccuucau gaaggcccug      660

cgcgagcgga uccucgccac caccggcgag guccuggacg acgggaucac ccccggccag      720

gcgauggccc agguggcccu gacgcacaac aucagcaugc gcgggccgga gggcauccuc      780

ggcaagcugc accaguccga caacgccaac gagauccgca agaucugcgc ccggcagggg      840

gugagccccg acgucugcaa gcagcugcuc cgcgcggugu ucaaggccga cagcccccgg      900

ggcuccgccg ugucccgcgu cgcccccgac ccgcugcccg ggcagggcag cuuccggcgc      960

gcccccaagu gcgaccccga guuccagcgg uuccgcauca ucuccaucgu ggcgaaccug     1020

cggaucagcg agaccaaggg ggagaaccgc ccgcucaccg ccgacgagcg gcgccacgug     1080

gucaccuucc ugacggagga cagccaggcc gaccugaccu ggguggacgu ggccgagaag     1140

cucggcgucc accggcgcga ccugcggggc accgccgugc acaccgacga cggggagcgc     1200

uccgcggccc ggccgcccau cgacgccacg gaccgcauca ugcggcagac caagaucagc     1260

ucccugaaga ccugguggga ggaggccgac agcgagcagc gcggcgcgau gauccgcuac     1320

cucuacgagg accccaccga cuccgagugc gccgagauca ucgccgagcu gcccgaggag     1380

gaccaggcca agcuggacag ccuccaccuc cccgccgggc gggcggccua cucccgcgag     1440

agccugacgg cccuguccga ccacaugcug gccaccaccg acgaccugca cgaggcccgg     1500

aagcgccucu ucggcgucga cgacagcugg gcgccgcccg ccgaggccau caacgccccc     1560

guggggaacc ccuccgugga ccggacccug aagaucgucg gccgcuaccu gagcgccgug     1620

gaguccaugu ggggcacgcc cgaggugauc cacgucgagc acgugcggga cggguucacc     1680

agcgagcgca uggcggacga gcgggacaag gccaaccgcc ggcgcuacaa cgacaaccag     1740

gaggccauga agaagaucca gcgggacuac ggcaaggagg gguacaucuc ccgcggcgac     1800

aucgugcggc ucgacgcccu ggagcugcag gggugcgcgu gccuguacug cggcaccacc     1860

aucggcuacc acacgugcca gcucgaccac aucgucccgc aggccgggcc cggcagcaac     1920

aaccgccggg ggaaccuggu ggccgugugc gagcgcugca accgguccaa gagcaacacc     1980

cccuucgccg ucugggccca gaagugcggc aucccccacg ugggcgugaa ggaggcgauc     2040

gggcgcgucc ggggcuggcg caagcagacc ccgaacaccu ccagcgagga ccugacgcgc     2100

cucaagaagg aggugaucgc ccggcugcgc cggacccagg aggaccccga gaucgacgag     2160

cgcagcaugg aguccguggc cuggauggcc aacgagcugc accaccggau cgccgcggcc     2220

uaccccgaga ccaccgucau gguguaccgc gggagcauca cggccgccgc ccggaaggcg     2280

gccggcaucg acucccgcau caaccucauc ggggagaagg gccggaagga ccgcaucgac     2340

cggcgccacc acgccgugga cgccagcguc guggcgcuga uggaggccuc cguggccaag     2400

acccuggccg agcggagcuc ccuccgcggc gagcagcggc ugaccgggaa ggagcagacc     2460

uggaagcagu acaccggcag cacggucggg gcccgcgagc acuucgagau guggcggggc     2520

cacaugcugc accucaccga gcuguucaac gagcgccugg cggaggacaa gguguacgug     2580

acccagaaca uccggcuccg ccuguccgac gggaacgccc acaccgucaa ccccagcaag     2640

cugguguccc accgccucgg cgacggccug acggugcagc agaucgaccg ggccugcacc     2700

ccggcccugu ggugcgcccu cacccgcgag aaggacuucg acgagaagaa cgggcugccc     2760

gcgcgggagg accgcgccau ccggguccac ggccacgaga ucaagagcuc cgacuacauc     2820

cagguguuca gcaagcgcaa gaagaccgac uccgaccggg acgagacgcc cuucggggcc     2880

aucgccgugc gcggcggguu cgucgagauc ggccccagca uccaccacgc ccggaucuac     2940

cgcguggagg gcaagaagcc gguguacgcg augcugcggg ucuucaccca cgaccuccug     3000

ucccagcgcc acggggaccu guucagcgcc gugaucccgc cccagagcau cuccaugcgg     3060

ugcgccgagc ccaagcuccg caaggccauc accaccggca acgccacgua ccuggggugg     3120

guggucgugg gcgacgagcu ggagaucaac guggacagcu ucaccaagua cgcgaucggg     3180

cgguuccucg aggacuuccc caacaccacc cgcuggcgga ucugcggcua cgacacgaac     3240

uccaagcuga cccugaagcc caucguccuc gccgccgagg gccuggagaa ccccagcucc     3300

gccgugaacg agaucgugga gcugaagggg uggcgcgucg cgaucaacgu gcucaccaag     3360

gugcacccca ccgucgugcg gcgcgacgcc cugggccggc cgcgcuacag cucccgcagc     3420

aaccugccca cguccuggac caucgagaag cggcccgccg ccaccaagaa ggccgggcag     3480

gcgaagaaga agaaguaagg acuagugucc accugucccu ccugggcugc uggauugucu     3540

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              3651


<210>  2511
<211>  4512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2511
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgacc aagcccuaca gcaucggccu ggacaucggg      180

accaacuccg ugggcugggc cgugacgacc gacaacuaca agguccccag caagaagaug      240

aaggugcucg ggaacaccuc caagaaguac aucaagaaga accugcuggg cgugcuccug      300

uucgacagcg gcaucaccgc cgaggggcgg cgccugaagc ggacggcccg ccggcgcuac      360

acccggcgcc ggaaccgcau ccucuaccug caggagaucu ucuccaccga gauggccacc      420

cuggacgacg cguucuucca gcggcucgac gacagcuucc ugguccccga cgacaagcgc      480

gacuccaagu acccgaucuu cggcaaccug guggaggaga aggccuacca cgacgaguuc      540

cccacgaucu accaccuccg gaaguaccug gccgacagca ccaagaaggc cgaccugcgc      600

cucguguacc uggcgcuggc ccacaugauc aaguaccggg ggcacuuccu caucgagggc      660

gaguucaacu ccaagaacaa cgacauccag aagaacuucc aggacuuccu ggacaccuac      720

aacgccaucu ucgagagcga ccugucccuc gagaacagca agcagcugga ggagaucguc      780

aaggacaaga ucuccaagcu ggagaagaag gaccgcaucc ucaagcuguu ccccggggag      840

aagaacagcg gcaucuucuc cgaguuccug aagcucaucg ugggcaacca ggccgacuuc      900

cggaagugcu ucaaccugga cgagaaggcc agccugcacu ucuccaagga gagcuacgac      960

gaggaccucg agacccugcu gggguacauc ggcgacgacu acuccgacgu guuccucaag     1020

gcgaagaagc uguacgacgc cauccugcuc agcggguucc ugacggucac cgacaacgag     1080

accgaggcgc cccugagcuc cgccaugauc aagcgcuaca acgagcacaa ggaggaccuc     1140

gcccugcuga aggaguacau ccggaacauc agccucaaga ccuacaacga gguguucaag     1200

gacgacacga agaacggcua cgccggcuac aucgacggga agaccaacca ggaggacuuc     1260

uacguguacc ugaagaagcu gcucgccgag uucgagggcg ccgacuacuu ccuggagaag     1320

aucgaccgcg aggacuuccu gcggaagcag cgcaccuucg acaacggguc caucccguac     1380

cagauccacc uccaggagau gcgcgccauc cuggacaagc aggccaaguu cuaccccuuc     1440

cuggcgaaga acaaggagcg gaucgagaag auccugaccu uccgcauccc cuacuacguc     1500

ggcccccucg cccgggggaa cagcgacuuc gccuggucca uccgcaagcg gaacgagaag     1560

aucacgccgu ggaacuucga ggacgugauc gacaaggaga gcuccgccga ggccuucauc     1620

aaccgcauga ccagcuucga ccuguaccug cccgaggaga aggugcuccc caagcacucc     1680

cugcuguacg agaccuucaa cgucuacaac gagcucacca aggugcgguu caucgcggag     1740

agcaugcgcg acuaccaguu ccuggacucc aagcagaaga aggacaucgu gcggcuguac     1800

uucaaggaca agcgcaaggu cacggacaag gacaucaucg aguaccucca cgccaucuac     1860

ggcuacgacg gcaucgagcu gaaggggauc gagaagcagu ucaacagcuc ccugagcacc     1920

uaccacgacc uccugaacau caucaacgac aaggaguucc uggacgacuc cagcaacgag     1980

gccaucaucg aggagaucau ccacacccuc accaucuucg aggaccggga gaugaucaag     2040

cagcgccugu ccaaguucga gaacaucuuc gacaagagcg ugcugaagaa gcucucccgg     2100

cgccacuaca cgggcugggg gaagcugagc gccaagcuga ucaacggcau ccgggacgag     2160

aaguccggga acaccauccu cgacuaccug aucgacgacg gcaucagcaa ccgcaacuuc     2220

augcagcuga uccacgacga cgcgcucagc uucaagaaga agauccagaa ggcccagauc     2280

aucggcgacg aggacaaggg gaacaucaag gaggugguca agucccugcc cggcagcccg     2340

gccaucaaga aggggauccu gcaguccauc aagaucgugg acgagcucgu caaggugaug     2400

ggcgggcgga agcccgagag caucgugguc gagauggccc gcgagaacca guacaccaac     2460

cagggcaagu ccaacagcca gcagcggcug aagcgccugg agaagucccu caaggagcug     2520

ggcagcaaga uccugaagga gaacaucccc gccaagcucu ccaagaucga caacaacgcg     2580

cugcagaacg accgccugua ccucuacuac cugcagaacg ggaaggacau guacaccggc     2640

gacgaccugg acaucgaccg gcucagcaac uacgacaucg accacaucau cccccaggcc     2700

uuccugaagg acaacuccau cgacaacaag gugcugguga gcuccgccag caaccgcggg     2760

aaguccgacg acgucccgag ccucgaggug gugaagaagc ggaagacguu cugguaccag     2820

cugcucaaga gcaagcugau cucccagcgc aaguucgaca accugaccaa ggccgagcgg     2880

ggcggccuga gccccgagga caaggccggg uucauccagc gccagcucgu cgagacccgg     2940

cagaucacca agcacguggc gcgccugcug gacgagaagu ucaacaacaa gaaggacgag     3000

aacaaccggg ccgugcgcac ggucaagauc aucacccuca aguccacccu ggugagccag     3060

uuccggaagg acuucgagcu guacaaggug cgcgagauca acgacuucca ccacgcccac     3120

gacgccuacc ucaacgccgu cguggcgucc gcccugcuga agaaguaccc caagcucgag     3180

cccgaguucg uguacggcga cuacccgaag uacaacagcu uccgggagcg caaguccgcc     3240

accgagaagg ucuacuucua cagcaacauc augaacaucu ucaagaaguc caucagccug     3300

gccgacgggc gggugaucga gcgcccccug aucgagguga acgaggagac cggcgagucc     3360

gucuggaaca aggagagcga ccucgcgacg gugcggcgcg ugcuguccua cccccagguc     3420

aacgugguga agaaggucga ggagcagaac cacgggcugg accggggcaa gcccaagggc     3480

cucuucaacg ccaaccugag cuccaagccg aagcccaaca gcaacgagaa ccuggugggg     3540

gccaaggagu accucgaccc caagaaguac ggcggguacg ccggcaucuc caacagcuuc     3600

accgugcugg ucaaggggac caucgagaag ggcgccaaga agaagaucac caacgugcug     3660

gaguuccagg gcaucuccau ccucgaccgc aucaacuacc ggaaggacaa gcugaacuuc     3720

cugcucgaga agggguacaa ggacaucgag cugaucaucg agcugccgaa guacagccuc     3780

uucgagcuga gcgacggcuc ccgccgcaug cuggcgagca uccucuccac gaacaacaag     3840

cgcggggaga uccacaaggg caaccagauc uuccugagcc agaaguucgu gaagcugcuc     3900

uaccacgcca agcggaucuc caacaccauc aacgagaacc accggaagua cgucgagaac     3960

cacaagaagg aguucgagga gcuguucuac uacauccugg aguucaacga gaacuacgug     4020

ggggccaaga agaacggcaa gcuccugaac agcgccuucc aguccuggca gaaccacagc     4080

aucgacgagc ugugcuccag cuucaucggc cccaccgggu ccgagcgcaa gggccucuuc     4140

gagcugacca gccggggguc cgccgcggac uucgaguucc ugggcgugaa gaucccccgc     4200

uaccgggacu acacgcccag cucccuccug aaggacgcca cccugaucca ccagagcguc     4260

accgggcucu acgagacccg caucgaccug gccaagcugg gcgagggcaa gcggccggcc     4320

gccacgaaga aggcggggca ggccaagaag aagaaguaag gacuaguguc caccuguccc     4380

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     4440

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaagaa uu                                                         4512


<210>  2512
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2512
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcggcc gccuucaagc ccaacagcau caacuacauc      180

cugggccucg acaucgggau cgccuccgug ggcugggcca ugguggagau cgacgaggag      240

gagaacccca uccggcugau cgaccugggg guccgcgugu ucgagcgggc ggaggugccc      300

aagaccggcg acagccucgc cauggcccgc cggcuggccc gcuccguccg gcgccugacc      360

cggcgccggg cgcaccgccu ccugcggacg cgccggcugc ucaagcgcga gggcgugcug      420

caggccgcca acuucgacga gaacgggcug aucaagagcc ucccgaacac ccccuggcag      480

cugcgggccg ccgcgcugga ccgcaagcuc acgccccugg agugguccgc cgugcugcuc      540

caccugauca agcaccgggg cuaccugagc cagcgcaaga acgaggggga gaccgccgac      600

aaggagcucg gcgcccugcu gaaggggguc gccggcaacg cgcacgcccu ccagaccggc      660

gacuuccgga cccccgccga gcuggcccug aacaaguucg agaaggaguc cgggcacauc      720

cgcaaccagc gcagcgacua cucccacacc uucagccgga aggaccucca ggccgagcug      780

auccuccugu ucgagaagca gaaggaguuc ggcaacccgc acguguccgg gggccugaag      840

gagggcaucg agacccugcu caugacgcag cgccccgcgc ugagcgggga cgccgugcag      900

aagaugcugg gccacugcac cuucgagccc gccgagccca aggccgccaa gaacaccuac      960

accgcggagc gguucaucug gcucacgaag cugaacaacc ugcgcauccu cgagcagggg     1020

uccgagcggc cgcugaccga caccgagcgc gccacccuga uggacgagcc cuaccgcaag     1080

agcaagcuca cguacgccca ggcccggaag cugcugggcc ucgaggacac cgcguucuuc     1140

aaggggcugc gguacggcaa ggacaacgcc gaggccucca cccugaugga gaugaaggcc     1200

uaccacgcca ucagccgcgc gcucgagaag gagggccuga aggacaagaa guccccgcug     1260

aaccugagcc ccgagcucca ggacgagauc gggaccgccu ucucccuguu caagacggac     1320

gaggacauca ccggccggcu gaaggaccgc auccagccgg agauccucga ggcccugcug     1380

aagcacauca gcuucgacaa guucguccag aucagccuca aggcccugcg gcgcaucgug     1440

ccccugaugg agcaggggaa gcgguacgac gaggccugcg cggagaucua cggcgaccac     1500

uacgggaaga agaacaccga ggagaagauc uaccucccgc ccauccccgc cgacgagauc     1560

cgcaaccccg ugguccugcg ggcccugucc caggcccgca aggugaucaa cggcgugguc     1620

cggcgcuacg gcagccccgc ccgcauccac aucgagaccg cgcgggaggu ggggaagucc     1680

uucaaggacc gcaaggagau cgagaagcgg caggaggaga accgcaagga ccgggagaag     1740

gccgccgcca aguuccgcga guacuucccc aacuucgugg gcgagcccaa gagcaaggac     1800

auccucaagc ugcggcugua cgagcagcag cacgggaagu gccucuacuc cggcaaggag     1860

aucaaccugg ggcgccugaa cgagaagggc uacgucgaga ucgaccacgc gcuccccuuc     1920

agccggacgu gggacgacuc cuucaacaac aaggugcugg ugcugggcag cgagaaccag     1980

aacaagggga accagacccc cuacgaguac uucaacggca aggacaacuc ccgcgagugg     2040

caggaguuca aggcccgggu cgagaccagc cgcuuccccc gguccaagaa gcagcgcauc     2100

cuccugcaga aguucgacga ggacggguuc aaggagcgga accugaacga cacccgcuac     2160

gugaaccggu uccucugcca guucgucgcc gaccgcaugc ggcugacggg caagggcaag     2220

aagcgcgugu ucgccagcaa cgggcagauc accaaccugc uccggggcuu cugggggcug     2280

cgcaaggugc gcgccgagaa cgaccggcac cacgcgcugg acgccgucgu gguggccugc     2340

uccaccgucg ccaugcagca gaagaucacc cgcuucgugc gguacaagga gaugaacgcc     2400

uucgacggca agacgaucga caaggagacc ggggaggugc uccaccagaa gacccacuuc     2460

ccgcagcccu gggaguucuu cgcgcaggag gucaugaucc gcguguucgg caagcccgac     2520

ggcaagccgg aguucgagga ggccgacacc cuggagaagc ugcggacgcu ccuggccgag     2580

aagcugagcu cccgccccga ggccgugcac gaguacguca cgccccucuu cgugagccgg     2640

gcccccaacc gcaagauguc cgggcagggc cacauggaga ccgucaagag cgcgaagcgg     2700

cuggacgagg ggguguccgu gcugcgcgug ccgcucaccc agcugaagcu gaaggaccuc     2760

gagaagaugg ucaaccggga gcgcgagccc aagcuguacg aggcccugaa ggcccggcuc     2820

gaggcccaca aggacgaccc cgcgaaggcc uucgccgagc ccuucuacaa guacgacaag     2880

gccggcaacc gcacccagca ggucaaggcc gugcgggugg agcaggucca gaagaccggg     2940

gugugggugc gcaaccacaa cggcaucgcg gacaacgcca ccauggugcg gguggacguc     3000

uucgagaagg gcgacaagua cuaccuggug ccgaucuaca gcuggcaggu cgccaagggg     3060

auccugcccg accgcgccgu ggugcagggc aaggacgagg aggacuggca gcucaucgac     3120

gacagcuuca acuucaaguu cucccugcac cccaacgacc ugguggaggu caucaccaag     3180

aaggcccgga uguucgggua cuucgcgagc ugccaccgcg gcaccgggaa caucaacauc     3240

cgcauccacg accucgacca caagaucggc aagaacggca uccuggaggg gaucggcgug     3300

aagacggccc uguccuucca gaaguaccag aucgacgagc ucgggaagga gauccggccc     3360

ugccgccuga agaagcggcc gcccgugcgc aagcggcccg ccgccaccaa gaaggccggc     3420

caggcgaaga agaagaagua aggacuagug uccaccuguc ccuccugggc ugcuggauug     3480

ucucguuuuc cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           3594


<210>  2513
<211>  4350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2513
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaag aagcccuaca ccaucggccu ggacaucggg      180

accaacagcg ugggcugggc cgugcucacg gaccaguacg accuggucaa gcggaagaug      240

aagaucgccg gggacuccga gaagaagcag aucaagaaga acuucugggg cgugcgccug      300

uucgacgagg gccagaccgc cgccgaccgg cgcauggcgc ggaccgcccg ccggcgcauc      360

gagcggcgcc ggaaccgcau cagcuaccuc caggggaucu ucgccgagga gauguccaag      420

accgacgcca acuucuucug ccggcugagc gacuccuucu acguggacaa cgagaagcgc      480

aacagccggc accccuucuu cgcgacgauc gaggaggagg ucgaguacca caagaacuac      540

cccaccaucu accaccuccg cgaggagcug gugaacucca gcgagaaggc cgaccugcgg      600

cugguguacc ucgcccuggc ccacaucauc aaguaccgcg gcaacuuccu gaucgagggg      660

gcccucgaca cccagaacac cuccgucgac ggcaucuaca agcaguucau ccagacguac      720

aaccaggugu ucgcgagcgg gaucgaggac ggcucccuga agaagcugga ggacaacaag      780

gacguggcca agauccucgu cgagaaggug acccggaagg agaagcugga gcgcauccug      840

aagcucuacc cgggcgagaa guccgccggg auguucgccc aguucaucag ccugaucgug      900

ggcagcaagg ggaacuucca gaagcccuuc gaccugaucg agaaguccga caucgagugc      960

gccaaggaca gcuacgagga ggaccucgag ucccugcugg cgcucaucgg cgacgaguac     1020

gccgagcugu ucgucgccgc caagaacgcc uacagcgcgg uggugcuguc cagcaucauc     1080

accgucgccg agaccgagac gaacgccaag cucagcgccu ccaugaucga gcgguucgac     1140

acccacgagg aggaccuggg cgagcugaag gccuucauca agcuccaccu ccccaagcac     1200

uacgaggaga ucuucagcaa caccgagaag cacggguacg cgggcuacau cgacgggaag     1260

accaagcagg ccgacuucua caaguacaug aagaugacgc uggagaacau cgagggcgcc     1320

gacuacuuca ucgccaagau cgagaaggag aacuuccugc gcaagcagcg caccuucgac     1380

aacggggcga ucccccacca gcugcaccug gaggagcucg aggccauccu gcaccagcag     1440

gccaaguacu acccguuccu gaaggagaac uacgacaaga ucaagucccu cgugaccuuc     1500

cggauccccu acuucguggg cccccuggcc aacggccaga gcgaguucgc cuggcugacc     1560

cgcaaggcgg acggggagau ccggcccugg aacaucgagg agaaggucga cuucggcaag     1620

uccgccgugg acuucaucga gaagaugacg aacaaggaca ccuaccuccc gaaggagaac     1680

gugcugccca agcacagccu gugcuaccag aaguaccucg ucuacaacga gcugaccaag     1740

gugcgcuaca ucaacgacca ggggaagacc uccuacuuca gcggccagga gaaggagcag     1800

aucuucaacg accuguucaa gcagaagcgg aaggucaaga agaaggaccu cgagcuguuc     1860

cugcgcaaca ugucccacgu ggagagcccc acgaucgagg ggcuggagga cuccuucaac     1920

agcuccuaca gcaccuacca cgaccuccug aaggugggca ucaagcagga gauccuggac     1980

aaccccguca acaccgagau gcucgagaac aucgugaaga uccugaccgu guucgaggac     2040

aagcggauga ucaaggagca gcugcagcag uucuccgacg uccucgacgg cguggugcug     2100

aagaagcugg agcgccggca cuacacgggg uggggccgcc ucagcgccaa gcugcugaug     2160

gggauccggg acaagcaguc ccaccucacc auccuggacu accugaugaa cgacgacggc     2220

cucaaccgca accugaugca gcugaucaac gacagcaacc ucuccuucaa gagcaucauc     2280

gagaaggagc aggucaccac cgccgacaag gacauccaga gcaucguggc cgaccuggcg     2340

ggguccccgg ccaucaagaa gggcauccug cagagccuca agaucgugga cgagcugguc     2400

uccgugaugg gcuacccgcc ccagacgauc guggucgaga uggcccggga gaaccagacc     2460

accgggaagg gcaagaacaa cagccgcccc cgguacaagu cccuggagaa ggccaucaag     2520

gaguucggga gccagauccu caaggagcac cccaccgaca accaggagcu gcgcaacaac     2580

cggcuguacc ucuacuaccu gcagaacggc aaggacaugu acacgggcca ggaccuggac     2640

auccacaacc ucuccaacua cgacaucgac cacaucgugc cccagagcuu caucaccgac     2700

aacuccaucg acaaccuggu gcugaccagc uccgccggga accgcgagaa gggcgacgac     2760

gucccgcccc ucgagaucgu gcggaagcgc aagguguucu gggagaagcu guaccagggg     2820

aaccugauga gcaagcgcaa guucgacuac cucaccaagg cggagcgggg cgggcugacc     2880

gaggccgaca aggcccgcuu cauccaccgg cagcuggucg agacgcgcca gaucaccaag     2940

aacguggcca acauccucca ccagcgguuc aacuacgaga aggacgacca cggcaacacc     3000

augaagcagg uccgcaucgu gacccugaag uccgcgcugg ugagccaguu ccggaagcag     3060

uuccagcucu acaaggugcg cgacgucaac gacuaccacc acgcccacga cgccuaccug     3120

aacggcgugg uggccaacac gcugcucaag gucuaccccc agcuggagcc cgaguucgug     3180

uacggggacu accaccaguu cgacugguuc aaggccaaca aggcgaccgc caagaagcag     3240

uucuacacca acaucaugcu guucuucgcc cagaaggacc ggaucaucga cgagaacggc     3300

gagauccucu gggacaagaa guaccuggac accgugaaga aggucauguc cuaccgccag     3360

augaacaucg ugaagaagac ggagauccag aagggggagu ucagcaaggc caccaucaag     3420

cccaagggca acuccagcaa gcugaucccc cggaagacca acugggaccc gaugaaguac     3480

gggggccucg acagccccaa cauggccuac gcggugguca ucgaguacgc caagggcaag     3540

aacaagcugg uguucgagaa gaagaucauc cgcgugacca ucauggagcg gaaggccuuc     3600

gagaaggacg agaaggccuu ccuggaggag cagggguacc gccagcccaa gguccucgcc     3660

aagcugccga aguacacgcu guacgagugc gaggagggcc ggcgccggau gcucgcgucc     3720

gccaacgagg cccagaaggg gaaccagcag guccugccca accaccuggu gacccuccug     3780

caccacgccg cgaacugcga ggugagcgac ggcaaguccc uggacuacau cgagagcaac     3840

cgcgagaugu ucgccgagcu ccuggcccac guguccgagu ucgccaagcg guacacccug     3900

gccgaggcga accucaacaa gaucaaccag cuguucgagc agaacaagga gggggacauc     3960

aaggccaucg cccagagcuu cguggaccug auggccuuca acgccauggg cgcgcccgcc     4020

uccuucaagu ucuucgagac cacgaucgag cgcaagcggu acaacaaccu caaggagcug     4080

cugaacagca ccaucaucua ccaguccauc accggccucu acgagagccg caagcgccug     4140

gacgacaagc ggcccgccgc caccaagaag gccgggcagg cgaagaagaa gaaguaagga     4200

cuagugucca ccugucccuc cugggcugcu ggauugucuc guuuuccugc caaauaaaca     4260

ggaucagcgc uuuacagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4350


<210>  2514
<211>  4383
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2514
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaag aagcccuaca gcaucggccu ggacaucggg      180

accaacuccg ugggcugggc cguggucacc gacgacuaca aggugcccgc caagaagaug      240

aaggugcucg ggaacacgga caagagccac aucgagaaga accugcuggg cgcccuccug      300

uucgacuccg gcaacaccgc cgaggaccgg cgccugaagc ggaccgcgcg ccggcgcuac      360

acccggcgcc ggaaccgcau ccucuaccug caggagaucu ucagcgagga gauggggaag      420

gucgacgacu ccuucuucca ccggcuggag gacagcuucc ucgugacgga ggacaagcgc      480

ggcgagcggc accccaucuu cgggaaccug gaggaggagg ugaaguacca cgagaacuuc      540

ccgaccaucu accaccuccg ccaguaccug gccgacaacc ccgagaaggu cgaccugcgg      600

cugguguacc ucgcccuggc ccacaucauc aaguuccgcg gccacuuccu gaucgagggg      660

aaguucgaca cccggaacaa cgacgugcag cgccucuucc aggaguuccu ggcggucuac      720

gacaacaccu ucgagaacuc cagccugcag gagcagaacg uccaggugga ggagauccuc      780

acggacaaga ucuccaagag cgccaagaag gaccgggugc ugaagcuguu ccccaacgag      840

aaguccaacg gccgcuucgc cgaguuccuc aagcugaucg ugggcaacca ggccgacuuc      900

aagaagcacu ucgagcugga ggagaaggcc ccgcuccagu ucagcaagga caccuacgag      960

gaggagcugg aggugcugcu cgcgcagauc ggggacaacu acgccgagcu guuccugucc     1020

gccaagaagc ucuacgacag cauccugcug uccggcaucc ucaccgucac cgacgugggg     1080

acgaaggccc cgcugagcgc cuccaugauc cagcgguaca acgagcacca gauggaccug     1140

gcgcagcuca agcaguucau ccgccagaag cugagcgaca aguacaacga gguguucucc     1200

gacgucagca aggacggcua cgccggcuac aucgacggga agaccaacca ggaggccuuc     1260

uacaaguacc ugaagggccu ccugaacaag aucgagggga gcggcuacuu ccuggacaag     1320

aucgagcgcg aggacuuccu ccggaagcag cgcaccuucg acaacggguc caucccccac     1380

cagauccacc uccaggagau gcgggccauc auccgccggc aggccgaguu cuaccccuuc     1440

cuggcggaca accaggaccg caucgagaag cugcugaccu uccggauccc cuacuacgug     1500

ggcccgcugg cccgcggcaa gagcgacuuc gccuggcugu cccgcaagag cgccgacaag     1560

aucacgcccu ggaacuucga cgagaucgug gacaaggagu ccagcgccga ggcguucauc     1620

aaccggauga ccaacuacga ccucuaccug cccaaccaga agguccugcc caagcacucc     1680

cuccuguacg agaaguucac cguguacaac gagcugacca aggucaagua caagacggag     1740

caggggaaga ccgccuucuu cgacgccaac augaagcagg agaucuucga cggcguguuc     1800

aagguguacc ggaaggucac caaggacaag cucauggacu uccuggagaa ggaguucgac     1860

gaguuccgca ucguggaccu gaccgggcuc gacaaggaga acaagguguu caacgccagc     1920

uacggcacgu accacgaccu gugcaagauc cuggacaagg acuuccucga caacuccaag     1980

aacgagaaga uccuggagga caucguccug acccucaccc uguucgagga ccgggagaug     2040

auccgcaagc ggcuggagaa cuacagcgac cuccugacca aggagcaggu caagaagcug     2100

gagcgccggc acuacacggg guggggccgc cucuccgcgg agcugaucca cggcauccgg     2160

aacaaggaga gccgcaagac cauccuggac uaccucaucg acgacgggaa cuccaaccgc     2220

aacuucaugc agcugaucaa cgacgacgcc cugagcuuca aggaggagau cgccaaggcc     2280

caggugaucg gcgagaccga caaccucaac cagguggugu ccgacaucgc cgggagcccg     2340

gcgaucaaga agggcauccu gcagucccug aagaucgugg acgagcucgu caagaucaug     2400

gggcaccagc ccgagaacau cgugguggag auggcccggg agaaccaguu caccaaccag     2460

ggccgccgga acagccagca gcgccugaag ggccugacgg acuccaucaa ggaguucggg     2520

agccagaucc ucaaggagca ccccgucgag aacagccagc ugcagaacga ccggcuguuc     2580

cucuacuacc ugcagaacgg ccgcgacaug uacaccgggg aggagcugga caucgacuac     2640

cucucccagu acgacaucga ccacaucauc ccccaggccu ucaucaagga caacagcauc     2700

gacaaccggg ugcugaccuc cagcaaggag aaccgcggca aguccgacga cgugccgagc     2760

aaggacgucg ugcggaagau gaaguccuac uggagcaagc ugcucuccgc caagcugauc     2820

acccagcgca aguucgacaa ccugacgaag gccgagcggg gcgggcucac cgacgacgac     2880

aaggcgggcu ucaucaagcg ccagcuggug gagacccggc agaucaccaa gcacgucgcc     2940

cgcauccugg acgagcgguu caacaccgag acggacgaga acaacaagaa gauccgccag     3000

gugaagaucg ugacccucaa gagcaaccug gucuccaacu uccggaagga guucgagcug     3060

uacaaggugc gcgagaucaa cgacuaccac cacgcccacg acgccuaccu caacgccgug     3120

aucgggaagg cgcugcuggg cgucuacccc cagcucgagc ccgaguucgu guacggggac     3180

uacccccacu uccacggcca caaggagaac aaggccaccg ccaagaaguu cuucuacagc     3240

aacaucauga acuucuucaa gaaggacgac gugcggaccg acaagaacgg cgagaucauc     3300

uggaagaagg acgagcacau cuccaacauc aagaaggucc ugagcuaccc ccaggugaac     3360

aucgugaaga aggucgagga gcagaccggg ggcuucucca aggagagcau ccugcccaag     3420

gggaacuccg acaagcucau cccccgcaag acgaagaagu ucuacuggga caccaagaag     3480

uacggcgggu ucgacagccc caucguggcc uacagcaucc uggugaucgc ggacaucgag     3540

aagggcaagu ccaagaagcu gaagaccguc aaggcccucg ugggcgugac gaucauggag     3600

aagaugaccu ucgagcggga cccggucgcc uuccuggagc gcaaggggua ccgcaacgug     3660

caggaggaga acaucaucaa gcugcccaag uacagccucu ucaagcugga gaacggccgg     3720

aagcgccugc ucgccuccgc ccgggagcug cagaagggga acgagaucgu gcugcccaac     3780

caccucggca cccugcugua ccacgcgaag aacauccaca aggucgacga gccgaagcac     3840

cucgacuacg uggacaagca caaggacgag uucaaggagc ugcuggacgu ggucagcaac     3900

uucuccaaga aguacacccu cgccgagggg aaccuggaga agaucaagga gcuguacgcc     3960

cagaacaacg gcgaggaccu caaggagcug gccagcuccu ucaucaaccu gcucacguuc     4020

accgccaucg gcgcgcccgc caccuucaag uucuucgaca agaacaucga ccgcaagcgg     4080

uacaccagca cgaccgagau ccugaacgcc acccugaucc accaguccau caccgggcuc     4140

uacgagacgc gcaucgaccu gaacaagcug ggcggggaca agcggcccgc cgccaccaag     4200

aaggcgggcc aggccaagaa gaagaaguaa ggacuagugu ccaccugucc cuccugggcu     4260

gcuggauugu cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     4380

auu                                                                   4383


<210>  2515
<211>  3516
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2515
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgcag accaccaacc ugagcuacau ccucggccug      180

gaccugggga ucgccuccgu gggcugggcc guggucgaga ucaacgagaa cgaggacccc      240

aucgggcuca ucgacguggg cgugcggauc uucgagcgcg ccgagguccc caagacgggc      300

gagagccugg cccugucccg gcgccucgcg cggagcaccc gccggcugau ccgccggcgc      360

gcccaccggc ugcuccuggc caagcgcuuc cugaagcggg aggggauccu cuccaccauc      420

gaccuggaga agggccugcc caaccaggcc ugggagcucc gcguggcggg gcuggagcgg      480

cgccugagcg ccaucgagug gggcgccgug cuccugcacc ugaucaagca ccggggguac      540

cucuccaagc gcaagaacga gagccagacc aacaacaagg agcugggcgc ccugcucucc      600

ggcgucgccc agaaccacca gcugcugcag agcgacgacu accggacgcc ggcggagcuc      660

gcccugaaga aguucgccaa ggaggagggg cacauccgca accagcgggg cgccuacacc      720

cacaccuuca accgccugga ccuccuggcc gagcugaacc uccuguucgc gcagcagcac      780

caguucggga acccccacug caaggagcac auccagcagu acaugaccga gcugcucaug      840

uggcagaagc ccgcccuguc cggcgaggcc auccugaaga ugcucggcaa gugcacgcac      900

gagaagaacg aguucaaggc cgccaagcac accuacagcg cggagcgcuu cguguggcug      960

accaagcuga acaaccuccg gauccuggag gacggggccg agcgcgcccu gaacgaggag     1020

gagcggcagc uccugaucaa ccaccccuac gagaagucca agcugaccua cgcccaggug     1080

cgcaagcucc ugggccugag cgagcaggcc aucuucaagc accuccggua cuccaaggag     1140

aacgcggaga gcgccacguu cauggagcuc aaggccuggc acgccauccg caaggcgcug     1200

gagaaccagg ggcugaagga caccuggcag gaccucgcca agaagccgga ccugcuggac     1260

gagaucggca ccgccuucuc ccucuacaag accgacgagg acauccagca guaccugacg     1320

aacaaggucc ccaacagcgu gaucaacgcc cugcucgugu cccugaacuu cgacaaguuc     1380

aucgagcuga gccucaagag ccugcggaag auccugcccc ucauggagca ggggaagcgc     1440

uacgaccagg ccugccggga gaucuacggc caccacuacg gcgaggcgaa ccagaagacc     1500

ucccagcugc ugcccgccau cccggcccag gagauccgca accccgucgu gcuccggacc     1560

cugagccagg cccgcaaggu gaucaacgcc aucauccggc aguacggguc ccccgcgcgc     1620

guccacaucg agaccggccg ggagcugggc aagagcuuca aggagcgccg ggagauccag     1680

aagcagcagg aggacaaccg cacgaagcgg gaguccgccg ugcagaaguu caaggagcug     1740

uucagcgacu ucuccagcga gcccaagucc aaggacaucc ugaaguuccg ccuguacgag     1800

cagcagcacg ggaagugccu cuacagcggg aaggagauca acauccaccg ccugaacgag     1860

aagggcuacg uggagaucga ccacgcccug ccguucuccc ggaccuggga cgacagcuuc     1920

aacaacaagg uccucgugcu ggccuccgag aaccagaaca agggcaacca gacccccuac     1980

gaguggcugc aggggaagau caacagcgag cgcuggaaga acuucguggc ccucguccug     2040

ggcucccagu gcagcgcggc caagaagcag cggcugcuca cccaggugau cgacgacaac     2100

aaguucaucg accgcaaccu gaacgacacg cgguacaucg cccgcuuccu guccaacuac     2160

auccaggaga accuccugcu ggucgggaag aacaagaaga acguguucac ccccaacggc     2220

cagaucaccg cccuccugcg gagccgcugg gggcugauca aggcgcggga gaacaacaac     2280

cgccaccacg cccucgacgc caucgugguc gccugcgcca cccccuccau gcagcagaag     2340

aucacgcggu ucauccgcuu caaggaggug cacccguaca agaucgagaa ccgguacgag     2400

augguggacc aggagagcgg cgagaucauc agcccccacu uccccgagcc cugggcguac     2460

uuccgccagg aggucaacau ccggguguuc gacaaccacc cggacaccgu gcugaaggag     2520

augcugcccg accgccccca ggccaaccac caguucgucc agccccucuu cgugucccgg     2580

gccccgaccc gcaagaugag cggccagggg cacauggaga ccaucaaguc cgccaagcgg     2640

cuggccgagg gcaucagcgu gcugcgcauc ccccucacgc agcugaagcc caaccugcuc     2700

gagaacaugg ucaacaagga gcgggagccg gcgcuguacg ccgggcugaa ggcccgccuc     2760

gccgaguuca accaggaccc cgccaaggcg uucgccaccc ccuucuacaa gcagggcggc     2820

cagcagguca aggccauccg cguggagcag guccagaagu ccggggugcu ggugcgggag     2880

aacaacggcg uggccgacaa cgcgagcauc gugcgcaccg acguguucau caagaacaac     2940

aaguucuucc ugguccccau cuacaccugg caggucgcca aggggauccu cccgaacaag     3000

gccaucgugg cccacaagaa cgaggacgag ugggaggaga uggacgaggg cgccaaguuc     3060

aaguucuccc uguuccccaa cgaccuggug gagcucaaga cgaagaagga guacuucuuc     3120

ggguacuaca ucggccugga ccgggcgacc ggcaacauca gccugaagga gcacgacggg     3180

gagaucucca agggcaagga cgggguguac cgcgugggcg ucaagcucgc ccugagcuuc     3240

gagaaguacc agguggacga gcuggggaag aaccggcaga ucugccgccc ccagcagcgg     3300

cagcccgugc gcaagcggcc ggccgccacc aagaaggccg gccaggcgaa gaagaagaag     3360

uaaggacuag uguccaccug ucccuccugg gcugcuggau ugucucguuu uccugccaaa     3420

uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3516


<210>  2516
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2516
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcggcc gccuucaagc ccaaccccau caacuacauc      180

cugggccucg acaucgggau cgccagcgug ggcugggcca ugguggagau cgacgaggac      240

gagaacccca ucugccugau cgaccugggg guccgggugu ucgagcgcgc ggaggugccg      300

aagaccggcg acucccucgc cauggcccgg cgccuggccc ggagcguccg ccggcugacc      360

cgccggcgcg cgcaccggcu ccugcgcgcc cggcgccugc ucaagcggga gggcgugcug      420

caggccgccg acuucgacga gaacgggcug aucaaguccc uccccaacac gcccuggcag      480

cugcgcgccg cggcccugga ccggaagcuc acgccccugg aguggagcgc cgugcugcuc      540

caccugauca agcaccgcgg cuaccugucc cagcggaaga acgaggggga gaccgccgac      600

aaggagcucg gcgcccugcu gaaggggguc gcggacaacg cccacgcccu ccagaccggc      660

gacuuccgca ccccggccga gcuggcccug aacaaguucg agaaggagag cggccacauc      720

cggaaccagc gcggggacua cucccacacc uucagccgca aggaccucca ggcggagcug      780

auccuccucu ucgagaagca gaaggaguuc ggcaaccccc acguguccgg gggccugaag      840

gagggcaucg agacccugcu caugacccag cggcccgccc ugagcgggga cgccgugcag      900

aagaugcugg gccacugcac guucgagccc gccgagccga aggccgcgaa gaacaccuac      960

accgccgagc gcuucaucug gcucaccaag cugaacaacc ugcggauccu cgagcagggg     1020

uccgagcgcc cccugacgga caccgagcgg gccacccuga uggacgagcc cuaccgcaag     1080

agcaagcuca ccuacgccca ggcgcggaag cugcugggcc ucgaggacac ggccuucuuc     1140

aaggggcugc gcuacggcaa ggacaacgcc gaggccucca cccugaugga gaugaaggcc     1200

uaccacgcga ucagccgggc ccucgagaag gagggccuga aggacaagaa guccccgcug     1260

aaccugagcc ccgagcucca ggacgagauc gggaccgccu ucucccuguu caagaccgac     1320

gaggacauca cgggccgccu gaaggaccgg auccagcccg agauccucga ggcccugcug     1380

aagcacauca gcuucgacaa guucguccag aucucccuca aggcccugcg ccggaucgug     1440

ccccugaugg agcaggggaa gcgcuacgac gaggcgugcg ccgagaucua cggcgaccac     1500

uacgggaaga agaacaccga ggagaagauc uaccugcccc cgauccccgc cgacgagauc     1560

cggaaccccg ugguccugcg cgcccugagc caggcccgga aggugaucaa cggcgugguc     1620

cgccgguacg gcagccccgc gcgcauccac aucgagaccg cccgcgaggu ggggaagucc     1680

uucaaggacc ggaaggagau cgagaagcgc caggaggaga accggaagga ccgcgagaag     1740

gccgccgcga aguuccggga guacuucccg aacuucgugg gcgagcccaa gagcaaggac     1800

auccucaagc ugcgccugua cgagcagcag cacgggaagu gccucuacuc cggcaaggag     1860

aucaaccugg ggcggcugaa cgagaagggc uacgucgaga ucgaccacgc ccuccccuuc     1920

agccgcaccu gggacgacuc cuucaacaac aaggugcugg ugcugggcag cgagaaccag     1980

aacaagggga accagacgcc cuacgaguac uucaacggca aggacaacuc ccgggagugg     2040

caggaguuca aggcccgcgu cgagaccagc cgguucccgc gcuccaagaa gcagcggauc     2100

cuccugcaga aguucgacga ggacggguuc aaggagcgca accugaacga cacccgguac     2160

gugaaccgcu uccucugcca guucgucgcc gaccggaugc gccugaccgg caagggcaag     2220

aagcgggugu ucgccagcaa cgggcagauc acgaaccugc uccgcggcuu cugggggcug     2280

cggaaggugc gcgcggagaa cgaccgccac cacgcccugg acgccgucgu gguggccugc     2340

uccaccgucg ccaugcagca gaagaucacc cgguucgugc gcuacaagga gaugaacgcg     2400

uucgacggca agaccaucga caaggagacg ggggaggugc uccaccagaa gacccacuuc     2460

ccccagcccu gggaguucuu cgcccaggag gucaugaucc ggguguucgg caagccggac     2520

ggcaagcccg aguucgagga ggccgacacc cccgagaagc ugcgcacccu gcucgccgag     2580

aagcugagcu cccggcccga ggccgugcac gaguacguca cgccgcuguu cgugagccgc     2640

gcgcccaacc ggaagauguc cgggcagggc cacauggaga ccgucaagag cgccaagcgc     2700

cucgacgagg ggguguccgu gcugcgggug ccccugaccc agcucaagcu gaaggaccug     2760

gagaagaugg ucaaccgcga gcgggagccc aagcucuacg aggcccugaa ggcccgccug     2820

gaggcgcaca aggacgaccc ggccaaggcc uucgccgagc ccuucuacaa guacgacaag     2880

gccggcaacc ggacccagca ggucaaggcg gugcgcgugg agcaggucca gaagaccggg     2940

gugugggugc ggaaccacaa cggcaucgcc gacaacgcca cgauggugcg cguggacguc     3000

uucgagaagg gcgacaagua cuaccucgug cccaucuaca gcuggcaggu cgccaagggg     3060

auccugcccg accgggccgu ggugcagggc aaggacgagg aggacuggca gcugaucgac     3120

gacuccuuca acuucaaguu cagccuccac ccgaacgacc ugguggaggu caucaccaag     3180

aaggcgcgca uguucgggua cuucgccagc ugccaccggg gcaccgggaa caucaacauc     3240

cgcauccacg accuggacca caagaucggc aagaacggca uccucgaggg gaucggcgug     3300

aagaccgccc uguccuucca gaaguaccag aucgacgagc uggggaagga gauccgcccc     3360

ugccggcuca agaagcgccc gcccgugcgg aagcgccccg ccgccacgaa gaaggcgggc     3420

caggccaaga agaagaagua aggacuagug uccaccuguc ccuccugggc ugcuggauug     3480

ucucguuuuc cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           3594


<210>  2517
<211>  3600
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2517
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgaag uaccacgugg gcaucgacgu ggggaccuuc      180

agcgucggcc uggccgccau cgagguggac gacgccggga ugcccaucaa gacccugucc      240

cuggugagcc acauccacga cuccggccug gaccccgacg agaucaagag cgccgucacg      300

cggcucgcgu ccagcggcau cgcccgccgg acccgccggc uguaccgccg gaagcgccgg      360

cgccugcagc agcucgacaa guucauccag cggcaggggu ggcccgugau cgagcuggag      420

gacuacuccg acccgcugua ccccuggaag gugcgcgccg agcucgccgc gagcuacauc      480

gccgacgaga aggagcgggg cgagaagcug uccgucgccc ugcgccacau cgcccggcac      540

cgcggguggc ggaaccccua cgccaaggug agcucccucu accugcccga cggcccgagc      600

gacgcguuca aggccauccg cgaggagauc aagcgggccu ccgggcagcc cgugcccgag      660

accgccaccg ucggccagau ggugacgcug ugcgagcucg gcacccugaa gcugcgcggg      720

gagggcgggg ugcucagcgc ccggcugcag caguccgacu acgcgcgcga gauccaggag      780

aucugccgca ugcaggagau cggccaggag cuguaccgga agaucaucga cgucguguuc      840

gccgccgaga gccccaaggg cuccgccagc ucccgcgugg ggaaggaccc gcuccagccc      900

ggcaagaacc gggcccugaa ggcgagcgac gccuuccagc gcuaccggau cgccgcccug      960

aucgggaacc uccgcguccg gguggacggc gagaagcgca uccugagcgu ggaggagaag     1020

aaccuggucu ucgaccaccu cgugaaccug acccccaaga aggagcccga gugggugacc     1080

aucgccgaga uccuggggau cgaccggggc cagcucaucg gcacggcgac caugaccgac     1140

gacggggagc gcgccggcgc ccggccgccc acccacgaca cgaaccgcuc caucgucaac     1200

agccggaucg cgccccuggu ggacuggugg aagaccgccu ccgcccugga gcagcacgcc     1260

auggucaagg cccucagcaa cgccgaggug gacgacuucg acucccccga gggggcgaag     1320

gugcaggccu ucuucgccga ccuggacgac gacguccacg ccaagcugga cagccuccac     1380

cucccggugg gccgcgccgc guacuccgag gacacccugg ugcggcucac ccgccggaug     1440

cugagcgacg gggucgaccu guacacggcc cgccuccagg aguucggcau cgagcccucc     1500

uggaccccgc ccacgccccg gaucggcgag cccgugggga accccgccgu ggaccgcguc     1560

cugaagaccg ugagccgcug gcuggagucc gccaccaaga ccuggggcgc ccccgagcgg     1620

gugaucaucg agcacguccg cgagggguuc gugaccgaga agcgggcgcg cgagauggac     1680

ggcgacaugc ggcgccgggc cgcccgcaac gccaagcugu uccaggagau gcaggagaag     1740

cugaacgugc aggggaagcc gagccgggcg gaccuguggc gcuaccaguc cguccagcgg     1800

cagaacugcc agugcgccua cugcggcagc cccaucaccu ucuccaacag cgagauggac     1860

cacaucgugc cccgcgccgg ccaggggucc acgaacaccc gggagaaccu cguggccguc     1920

ugccaccgcu gcaaccagag caagggcaac accccguucg ccaucugggc gaagaacacc     1980

uccaucgagg gggugagcgu gaaggaggcc gucgagcgga cgcgccacug ggugaccgac     2040

accggcaugc ggagcaccga cuucaagaag uucacgaagg ccguggucga gcgcuuccag     2100

cgggccacca uggacgagga gaucgacgcc cgcuccaugg agagcguggc guggauggcc     2160

aacgagcugc ggucccgcgu ggcccagcac uucgccagcc acggcaccac cguccgcgug     2220

uaccgggggu cccugacggc cgaggcgcgc cgggccagcg gcaucuccgg gaagcugaag     2280

uucuucgacg gcgugggcaa gagccgccuc gaccggcgcc accacgccau cgacgccgcg     2340

gucaucgccu ucaccuccga cuacguggcc gagacccugg ccgugcggag caaccugaag     2400

cagucccagg cccaccgcca ggaggcgccc caguggcggg aguucaccgg gaaggacgcc     2460

gagcaccgcg ccgccuggcg ggucuggugc cagaagaugg agaagcucag cgcccugcug     2520

accgaggacc uccgcgacga ccggguggug gucaugucca acgugcgccu gcggcugggc     2580

aacgggagcg cgcacaagga gacgaucggc aagcucucca aggugaagcu gagcucccag     2640

cugagcguca gcgacaucga caaggccucc agcgaggccc ucuggugcgc ccugacccgc     2700

gagcccgggu ucgaccccaa ggagggccug ccggccaacc ccgagcggca cauccgcgug     2760

aacgggaccc acguguacgc gggcgacaac aucggccucu uccccgucuc cgccgggagc     2820

aucgcccugc ggggcgggua cgccgagcug ggcuccagcu uccaccacgc ccgcguguac     2880

aagaucaccu ccgggaagaa gcccgcguuc gccaugcucc gcguguacac gaucgaccug     2940

cugccguacc ggaaccagga ccucuucagc gucgagcuga agccccagac cauguccaug     3000

cgccaggccg agaagaagcu gcgggacgcc cucgcgaccg gcaacgccga guaccugggc     3060

uggcuggugg uggacgacga gcucgucgug gacaccagca agaucgccac ggaccaggug     3120

aaggccgucg aggccgagcu ggggaccauc cgccgguggc gcguggacgg cuucuucucc     3180

cccagcaagc ugcggcuccg cccccugcag auguccaagg aggggaucaa gaaggagagc     3240

gcgccggagc uguccaagau caucgaccgg cccggcuggc uccccgccgu caacaagcug     3300

uucagcgacg ggaacgugac cgugguccgc cgggacuccc ugggccgcgu gcggcucgag     3360

agcaccgccc accuccccgu gacguggaag guccagaagc gcccggccgc caccaagaag     3420

gcgggccagg ccaagaagaa gaaguaagga cuagugucca ccugucccuc cugggcugcu     3480

ggauugucuc guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3600


<210>  2518
<211>  3300
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2518
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcggcc cggauccugg ccuucgacau cggcaucagc      180

uccaucgggu gggccuucag cgagaacgac gagcucaagg acugcggcgu gcgcaucuuc      240

accaaggugg agaaccccaa gaccggggag ucccuggccc ugccccggcg ccucgcgcgg      300

agcgcccgca agcggcuggc ccgccggaag gcccgccuga accaccucaa gcaccugauc      360

gcgaacgagu ucaagcugaa cuacgaggac uaccaguccu ucgacgagag ccucgccaag      420

gccuacaagg gcucccugau cagccccuac gagcugcggu uccgcgcccu caacgagcug      480

cuguccaagc aggacuucgc ccgggucauc cuccacaucg cgaagcgccg gggcuacgac      540

gacaucaaga acagcgacga caaggagaag ggggccaucc ugaaggccau caagcagaac      600

gaggagaagc uggccaacua ccaguccgug ggcgaguacc ucuacaagga guacuuccag      660

aaguucaagg agaacagcaa ggaguucacg aacgugcgca acaagaagga guccuacgag      720

cggugcaucg cccagagcuu ccugaaggac gagcugaagc ucaucuucaa gaagcagcgc      780

gaguucgggu ucuccuucag caagaaguuc gaggaggagg uccuguccgu ggcguucuac      840

aagcgggccc ugaaggacuu cagccaccuc gugggcaacu gcuccuucuu caccgacgag      900

aagcgcgccc cgaagaacag cccccuggcc uucauguucg ucgcccugac ccggaucauc      960

aaccuccuga acaaccugaa gaacaccgag gggauccucu acacgaagga cgaccugaac     1020

gcgcugcuca acgaggugcu gaagaacggc acccugaccu acaagcagac caagaagcuc     1080

cugggccuga gcgacgacua cgaguucaag ggggagaagg gcacguacuu caucgaguuc     1140

aagaaguaca aggaguucau caaggcccuc ggggagcaca accuguccca ggacgaccug     1200

aacgagaucg ccaaggacau cacccucauc aaggacgaga ucaagcugaa gaaggcccug     1260

gccaaguacg accucaacca gaaccagauc gacagccugu ccaagcugga guucaaggac     1320

caccucaaca ucagcuucaa ggcgcugaag cuggugacgc cccugaugcu cgagggcaag     1380

aaguacgacg aggccugcaa cgagcugaac cugaaggucg ccaucaacga ggacaagaag     1440

gacuuccucc ccgccuucaa cgagaccuac uacaaggacg aggugaccaa cccggugguc     1500

cugcgcgcga ucaaggagua ccgcaaggug cugaacgccc uccugaagaa guacggcaag     1560

gugcacaaga ucaacaucga gcuggcccgg gaggucggga agaaccacuc ccagcgcgcc     1620

aagaucgaga aggagcagaa cgagaacuac aaggccaaga aggacgcgga gcucgagugc     1680

gagaagcugg gccugaagau caacagcaag aacauccuca agcugcggcu guucaaggag     1740

cagaaggagu ucugcgccua cuccggggag aagaucaaga ucagcgaccu ccaggacgag     1800

aagaugcugg agaucgacca caucuacccc uacucccgca gcuucgacga cuccuacaug     1860

aacaaggugc ugguguucac caagcagaac caggagaagc ucaaccagac ccccuucgag     1920

gccuucggca acgacagcgc caaguggcag aagaucgagg uccuggccaa gaaccugccc     1980

accaagaagc agaagcggau ccucgacaag aacuacaagg acaaggagca gaagaacuuc     2040

aaggaccgca accugaacga cacgcgguac aucgcgcgcc uggugcucaa cuacaccaag     2100

gacuaccugg acuuccugcc gcucuccgac gacgagaaca ccaagcugaa cgacacccag     2160

aaggggagca aggugcacgu cgaggccaag uccggcaugc ugacgagcgc ccuccggcac     2220

accuggggcu ucuccgccaa ggaccgcaac aaccaccucc accacgccau cgacgcggug     2280

aucaucgccu acgccaacaa cagcaucguc aaggccuucu ccgacuucaa gaaggagcag     2340

gagagcaaca gcgcggagcu guacgccaag aagaucuccg agcuggacua caagaacaag     2400

cggaaguucu ucgagcccuu cagcggguuc cgccagaagg ugcuggacaa gaucgacgag     2460

aucuucgugu ccaagcccga gcggaagaag cccagcggcg cccugcacga ggagaccuuc     2520

cgcaaggagg aggaguucua ccaguccuac gggggcaagg aggggguccu caaggcccug     2580

gagcugggca agauccggaa ggugaacggc aagaucguga agaacgggga cauguuccgc     2640

gucgacaucu ucaagcacaa gaagaccaac aaguucuacg ccgugccgau cuacacgaug     2700

gacuucgcgc ucaaggugcu gcccaacaag gccgucgccc ggagcaagaa gggcgagauc     2760

aaggacugga uccugaugga cgagaacuac gaguucugcu ucucccucua caaggacagc     2820

cugauccuga uccagaccaa ggacaugcag gagcccgagu ucguguacua caacgccuuc     2880

accuccagca ccgugucccu caucgucagc aagcacgaca acaaguucga gacgcugucc     2940

aagaaccaga agauccuguu caagaacgcc aacgagaagg aggugaucgc caagagcauc     3000

gggauccaga accucaaggu guucgagaag uacaucgucu ccgcgcuggg cgaggugacc     3060

aaggccgagu uccgccagcg ggaggacuuc aagaagaagc gccccgccgc caccaagaag     3120

gcggggcagg ccaagaagaa gaaguaagga cuagugucca ccugucccuc cugggcugcu     3180

ggauugucuc guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3300


<210>  2519
<211>  3711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2519
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgagc gaccuggugc ucggccugga caucgggauc      180

ggcuccgugg gggucggcau ccugaacaag gugaccggcg agaucaucca caagaacagc      240

cggaucuucc ccgccgccca ggccgagaac aaccucgugc gccggaccaa ccgccagggg      300

cggcgccugg cccggcgcaa gaagcaccgg cgcguccggc ugaaccgccu cuucgaggag      360

uccggccuga ucacggacuu caccaagauc agcaucaacc ugaaccccua ccagcuccgg      420

gugaaggggc ugaccgacga gcuguccaac gaggagcugu ucaucgcgcu gaagaacaug      480

gugaagcacc gcggcaucag cuaccuggac gacgccuccg acgacgggaa cagcuccguc      540

ggcgacuacg cccagaucgu gaaggagaac agcaagcagc ucgagaccaa gacgcccggc      600

cagauccagc uggagcggua ccagaccuac gggcagcugc gcggcgacuu caccguggag      660

aaggacggga agaagcaccg gcucaucaac gucuucccga ccuccgccua ccgcagcgag      720

gcgcugcgga uccugcagac gcagcaggag uucaaccccc agaucaccga cgaguucauc      780

aaccgcuacc ucgagauccu gaccggcaag cggaaguacu accacggccc cgggaacgag      840

aagucccgca ccgacuacgg ccgcuaccgg acgagcgggg agacccugga caacaucuuc      900

ggcauccuca ucgggaagug caccuucuac cccgacgagu uccgcgccgc caaggccucc      960

uacaccgccc aggaguucaa ccugcugaac gaccucaaca accugacggu gccgaccgag     1020

accaagaagc ugagcaagga gcagaagaac cagaucauca acuacgugaa gaacgagaag     1080

gcgaugggcc ccgccaagcu guucaaguac aucgccaagc uccuguccug cgacgucgcc     1140

gacaucaagg gcuaccggau cgacaagagc gggaaggccg agauccacac cuucgaggcg     1200

uaccgcaaga ugaagacgcu cgagacccug gacaucgagc agauggaccg ggagacccug     1260

gacaagcucg ccuacgugcu gacccugaac acggagcgcg agggcaucca ggaggcccuc     1320

gagcacgagu ucgccgacgg guccuucagc cagaagcagg ucgacgagcu ggugcaguuc     1380

cggaaggcca acagcuccau cuucggcaag ggguggcaca acuucagcgu gaagcugaug     1440

auggagcuca uccccgagcu guacgagacc uccgaggagc agaugaccau ccugacccgc     1500

cucggcaagc agaagacgac cagcuccagc aacaagacca aguacaucga cgagaagcug     1560

cugaccgagg agaucuacaa ccccgugguc gcgaaguccg ugcggcaggc caucaagauc     1620

gugaacgccg ccaucaagga guacggcgac uucgacaaca ucgucaucga gauggcccgc     1680

gagacgaacg aggacgacga gaagaaggcg auccagaaga uccagaaggc caacaaggac     1740

gagaaggacg ccgccaugcu caaggcggcc aaccaguaca acgggaaggc cgagcugccg     1800

cacagcgugu uccacggcca caagcagcug gccaccaaga uccggcugug gcaccagcag     1860

ggggagcgcu gccucuacac cggcaagacc aucuccaucc acgaccugau caacaacagc     1920

aaccaguucg aggucgacca cauccugccc cucuccauca cguucgacga cagccuggcc     1980

aacaaggugc ugguguacgc gaccgccaac caggagaagg ggcagcggac ccccuaccag     2040

gcccucgacu ccauggacga cgccuggagc uuccgcgagc ugaaggccuu cguccgggag     2100

uccaagaccc ugagcaacaa gaagaaggag uaccuccuga ccgaggagga caucuccaag     2160

uucgacgugc gcaagaaguu caucgagcgg aaccuggugg acacgcgcua cgcgagccgg     2220

gucgugcuca acgcccugca ggagcacuuc cgcgcccaca agaucgacac caaggugucc     2280

gucgugcgcg gccaguucac cagccagcug cggcgccacu ggggcaucga gaagacccgg     2340

gacacguacc accaccacgc cguggacgcc cucaucaucg cggccagcuc ccagcugaac     2400

cuguggaaga agcagaagaa cacccucguc agcuacuccg aggaccagcu gcuggacauc     2460

gagaccgggg agcucaucag cgacgacgag uacaaggagu ccguguucaa ggcccccuac     2520

cagcacuucg uggacacccu caagagcaag gaguucgagg acuccauccu guucagcuac     2580

caggucgacu ccaaguucaa ccgcaagauc agcgacgcca cgaucuacgc gacccggcag     2640

gccaaggugg gcaaggacaa ggccgacgag accuacgugc ucgggaagau caaggacauc     2700

uacacccagg acggcuacga cgccuucaug aagaucuaca agaaggacaa guccaaguuc     2760

cugauguacc gccacgaccc gcagacguuc gagaagguca ucgagcccau ccuggagaac     2820

uaccccaaca agcagaucaa cgagaagggc aaggaggugc ccugcaaccc guuccucaag     2880

uacaaggagg agcacgggua cauccggaag uacagcaaga agggcaacgg gcccgagauc     2940

aagucccuga aguacuacga cagcaagcug ggcaaccaca ucgacaucac ccccaaggac     3000

uccaacaaca aggugguccu ccagagcgug ucccccuggc gcgccgacgu guacuucaac     3060

aagaccaccg ggaaguacga gauccugggc cugaaguacg cggaccucca guucgagaag     3120

ggcacgggga ccuacaagau cagccaggag aaguacaacg acaucaagaa gaaggagggc     3180

gucgacagcg acuccgaguu caaguucacc cuguacaaga acgaccugcu ccuggugaag     3240

gacaccgaga cgaaggagca gcagcuguuc cgguuccuca gccgcaccau gccgaagcag     3300

aagcacuacg uggagcugaa gcccuacgac aagcagaagu ucgagggcgg ggaggcccug     3360

aucaaggucc ucgggaacgu ggccaacucc ggccagugca agaagggccu gggcaagagc     3420

aacaucucca ucuacaaggu gcggaccgac guccugggga accagcacau caucaagaac     3480

gagggggaca agcccaagcu cgacuucaag cgccccgccg ccaccaagaa ggcgggccag     3540

gccaagaaga agaaguaagg acuagugucc accugucccu ccugggcugc uggauugucu     3600

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              3711


<210>  2520
<211>  4269
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2520
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgacc caguucgagg gcuucaccaa ccuguaccag      180

gugagcaaga cgcuccgguu cgagcugauc ccccagggga agacccugaa gcacauccag      240

gagcagggcu ucaucgagga ggacaaggcc cgcaacgacc acuacaagga gcucaagccc      300

aucaucgacc ggaucuacaa gaccuacgcc gaccagugcc ugcagcuggu gcagcucgac      360

ugggagaacc uguccgccgc caucgacagc uaccgcaagg agaagaccga ggagacgcgg      420

aacgcgcuga ucgaggagca ggccaccuac cgcaacgcca uccacgacua cuucaucggg      480

cggaccgaca accucaccga cgccaucaac aagcgccacg cggagaucua caagggccug      540

uucaaggccg agcuguucaa cggcaagguc cucaagcagc uggggacggu gaccaccacc      600

gagcacgaga acgcccugcu ccgguccuuc gacaaguuca cgaccuacuu cagcggcuuc      660

uacgagaacc gcaagaacgu guucuccgcc gaggacauca gcaccgccau cccccaccgg      720

aucguccagg acaacuuccc gaaguucaag gagaacugcc acaucuucac ccgccugauc      780

acggcggugc ccucccugcg ggagcacuuc gagaacguga agaaggccau cgggaucuuc      840

gucagcaccu ccaucgagga gguguucagc uuccccuucu acaaccagcu ccugacccag      900

acccagaucg accuguacaa ccagcuccug ggcgggaucu cccgcgaggc cggcacggag      960

aagaucaagg gccugaacga ggugcucaac cuggccaucc agaagaacga cgagaccgcc     1020

cacaucaucg cgagccugcc ccaccgguuc aucccccucu ucaagcagau ccuguccgac     1080

cgcaacaccc ugagcuucau ccucgaggag uucaaguccg acgaggaggu cauccagagc     1140

uucugcaagu acaagacccu gcugcggaac gagaacgugc ucgagacggc cgaggcccug     1200

uucaacgagc ugaacuccau cgaccucacc cacaucuuca ucagccacaa gaagcuggag     1260

accaucucca gcgcccugug cgaccacugg gacacccucc gcaacgcccu guacgagcgg     1320

cgcaucagcg agcugacggg gaagaucacc aaguccgcga aggagaaggu gcagcggagc     1380

cucaagcacg aggacaucaa ccugcaggag aucaucuccg ccgccggcaa ggagcugagc     1440

gaggccuuca agcagaagac cuccgagauc cucagccacg cccacgcggc ccuggaccag     1500

ccgcugccca ccacgcucaa gaagcaggag gagaaggaga uccugaaguc ccagcuggac     1560

agccugcucg ggcuguacca ccuccucgac ugguucgccg ucgacgaguc caacgaggug     1620

gaccccgagu ucagcgcccg ccugaccggc aucaagcugg agauggagcc gucccucagc     1680

uucuacaaca aggcgcgcaa cuacgccacc aagaagcccu acuccgugga gaaguucaag     1740

cugaacuucc agaugcccac ccuggccagc ggcugggacg ucaacaagga gaagaacaac     1800

ggggccaucc ucuucgugaa gaacggccug uacuaccugg ggaucaugcc caagcagaag     1860

ggccgguaca aggcccucuc cuucgagccg acggagaaga ccagcgaggg guucgacaag     1920

auguacuacg acuacuuccc cgacgcggcc aagaugaucc ccaagugcuc cacccagcug     1980

aaggccguga ccgcccacuu ccagacccac acgaccccca uccugcucag caacaacuuc     2040

aucgagccgc uggagaucac caaggagauc uacgaccuga acaaccccga gaaggagccc     2100

aagaaguucc agaccgccua cgcgaagaag acgggcgacc agaagggcua ccgcgaggcc     2160

cucugcaagu ggaucgacuu cacccgggac uuccugucca aguacaccaa gaccacgagc     2220

aucgaccuga gcucccuccg ccccagcucc caguacaagg accuggggga guacuacgcc     2280

gagcugaacc cgcuccugua ccacaucagc uuccagcgga ucgccgagaa ggagaucaug     2340

gacgccgucg agaccggcaa gcuguaccuc uuccagaucu acaacaagga cuucgcgaag     2400

gggcaccacg gcaagcccaa ccugcacacc cuguacugga ccggccucuu cucccccgag     2460

aaccuggcca agacgagcau caagcugaac gggcaggccg agcuguucua ccgccccaag     2520

ucccggauga agcgcauggc ccaccggcug ggcgagaaga ugcugaacaa gaagcucaag     2580

gaccagaaga ccccgauccc cgacacccug uaccaggagc uguacgacua cgugaaccac     2640

cgccucagcc acgaccuguc cgacgaggcg cgggcccugc uccccaacgu gaucaccaag     2700

gaggucagcc acgagaucau caaggaccgc cgguucacgu ccgacaaguu cuucuuccac     2760

gugccgauca cccugaacua ccaggccgcc aacagccccu ccaaguucaa ccagcgcguc     2820

aacgccuacc ugaaggagca ccccgagacc cccaucaucg ggaucgaccg gggcgagcgc     2880

aaccucaucu acaucaccgu gaucgacagc acggggaaga uccuggagca gcggucccug     2940

aacaccaucc agcaguucga cuaccagaag aagcucgaca accgcgagaa ggagcgggug     3000

gcggcccgcc aggccuggag cgucgugggc accaucaagg accugaagca gggguaccug     3060

ucccagguga uccacgagau cgucgaccuc augauccacu accaggccgu ggugguccug     3120

gagaaccuga acuucggcuu caagagcaag cgcaccggca ucgccgagaa ggcgguguac     3180

cagcaguucg agaagaugcu caucgacaag cugaacugcc uggugcucaa ggacuacccg     3240

gccgagaagg ucgggggcgu gcugaacccc uaccagcuga cggaccaguu caccagcuuc     3300

gccaagaugg ggacccaguc cggcuuccuc uucuacgugc ccgcccccua caccagcaag     3360

aucgacccgc ugacgggcuu cgucgacccc uucgugugga agaccaucaa gaaccacgag     3420

ucccggaagc acuuccugga gggguucgac uuccuccacu acgacgugaa gaccggcgac     3480

uucauccugc acuucaagau gaaccgcaac cugagcuucc agcgggggcu ccccggcuuc     3540

augcccgccu gggacaucgu cuucgagaag aacgagaccc aguucgacgc gaaggggacg     3600

ccguucaucg ccggcaagcg caucgugccc gugaucgaga accaccgguu caccggccgc     3660

uaccgggacc uguaccccgc caacgagcug aucgcccucc uggaggagaa ggggaucguc     3720

uuccgcgacg gcuccaacau ccugcccaag cuccuggaga acgacgacag ccacgcgauc     3780

gacaccaugg uggcccugau ccgguccgug cuccagaugc gcaacagcaa cgccgccacc     3840

ggggaggacu acaucaacuc cccgguccgg gaccugaacg gcgugugcuu cgacagccgc     3900

uuccagaacc ccgaguggcc cauggacgcc gacgcgaacg gggccuacca caucgcccug     3960

aagggccagc uccugcugaa ccaccucaag gaguccaagg accugaagcu gcagaacggc     4020

aucagcaacc aggacuggcu cgccuacauc caggagcugc ggaacaagcg ccccgccgcg     4080

acgaagaagg ccgggcaggc caagaagaag aaguaaggac uaguguccac cugucccucc     4140

ugggcugcug gauugucucg uuuuccugcc aaauaaacag gaucagcgcu uuacagaucu     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaagaauu                                                             4269


<210>  2521
<211>  4248
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2521
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa gaagcgcaag      120

gugggcaucc acgggguccc ggccgcgagc aucuaccagg aguucgugaa caaguacucc      180

cugagcaaga cccuccgguu cgagcugauc ccccagggca agacccugga gaacaucaag      240

gcccgcgggc ucauccugga cgacgagaag cgggccaagg acuacaagaa ggccaagcag      300

aucaucgaca aguaccacca guucuucauc gaggagaucc uguccagcgu gugcaucucc      360

gaggaccucc ugcagaacua cagcgacguc uacuucaagc ugaagaaguc cgacgacgac      420

aaccuccaga aggacuucaa gagcgccaag gacacgauca agaagcagau cuccgaguac      480

aucaaggaca gcgagaaguu caagaaccug uucaaccaga accugaucga cgcgaagaag      540

ggccaggagu ccgaccucau ccuguggcug aagcagagca aggacaacgg gaucgagcug      600

uucaaggcca acuccgacau caccgacauc gacgaggccc ucgagaucau caagagcuuc      660

aagggcugga ccaccuacuu caagggcuuc cacgagaacc gcaagaacgu guacuccagc      720

aacgacaucc ccacguccau caucuaccgg aucguggacg acaaccugcc caaguuccuc      780

gagaacaagg ccaaguacga gagccugaag gacaaggcgc cggaggccau caacuacgag      840

cagaucaaga aggaccuggc cgaggagcuc accuucgaca ucgacuacaa gaccuccgag      900

gucaaccagc gcguguucag ccuggacgag guguucgaga ucgccaacuu caacaacuac      960

cugaaccaga gcgggaucac caaguucaac acgaucaucg gcgggaaguu cgucaacggc     1020

gagaacacca agcggaaggg gaucaacgag uacaucaacc ucuacuccca gcagaucaac     1080

gacaagaccc ugaagaagua caagaugagc gugcuguuca agcagauccu cuccgacacc     1140

gagagcaagu ccuucgugau cgacaagcug gaggacgaca gcgacgucgu gacgaccaug     1200

caguccuucu acgagcagau cgccgcguuc aagaccgugg aggagaaguc caucaaggag     1260

acccuguccc uccuguucga cgaccugaag gcccagaagc ucgaccugag caagaucuac     1320

uucaagaacg acaagucccu gacggaccuc agccagcagg ucuucgacga cuacuccgug     1380

aucggcaccg ccgugcugga guacaucacc cagcagaucg cccccaagaa ccuggacaac     1440

cccagcaaga aggagcagga gcucaucgcc aagaagaccg agaaggcgaa guaccugucc     1500

cuggagacga ucaagcucgc ccuggaggag uucaacaagc accgcgacau cgacaagcag     1560

ugccgguucg aggagauccu ggccaacuuc gccgccaucc ccaugaucuu cgacgagauc     1620

gcgcagaaca aggacaaccu cgcccagauc agcaucaagu accagaacca gggcaagaag     1680

gaccugcugc aggccuccgc cgaggacgac gucaaggcca ucaaggaccu ccuggaccag     1740

accaacaacc ugcugcacaa gcucaagauc uuccacauca gccaguccga ggacaaggcg     1800

aacauccugg acaaggacga gcacuucuac cugguguucg aggagugcua cuucgagcuc     1860

gccaacaucg ugccgcugua caacaagauc cgcaacuaca ucacccagaa gcccuacagc     1920

gacgagaagu ucaagcugaa cuucgagaac agcacccucg ccaacgggug ggacaagaac     1980

aaggagcccg acaacacggc cauccuguuc aucaaggacg acaaguacua ccugggcguc     2040

augaacaaga agaacaacaa gaucuucgac gacaaggcga ucaaggagaa caagggggag     2100

ggcuacaaga agaucgugua caagcuccug cccggcgcca acaagaugcu gccgaaggug     2160

uucuucuccg ccaagagcau caaguucuac aaccccuccg aggacauccu ccggauccgc     2220

aaccacagca cccacaccaa gaacgggagc ccccagaagg gcuacgagaa guucgaguuc     2280

aacaucgagg acugccggaa guucaucgac uucuacaagc agagcaucuc caagcacccc     2340

gaguggaagg acuucggguu ccgcuucagc gacacccagc gguacaacuc caucgacgag     2400

uucuaccgcg aggucgagaa ccagggcuac aagcugacgu ucgagaacau cagcgagucc     2460

uacaucgaca gcguggucaa ccaggggaag cuguaccucu uccagaucua caacaaggac     2520

uucuccgccu acagcaaggg ccggccgaac cugcacaccc uguacuggaa ggcccucuuc     2580

gacgagcgca accugcagga cgugguguac aagcugaacg gcgaggcgga gcuguucuac     2640

cggaagcagu ccauccccaa gaagaucacc caccccgcca aggaggccau cgccaacaag     2700

aacaaggaca accccaagaa ggagagcguc uucgaguacg accucaucaa ggacaagcgc     2760

uucaccgagg acaaguucuu cuuccacugc ccgaucacga ucaacuucaa guccagcggg     2820

gccaacaagu ucaacgacga gaucaaccug cuccugaagg agaaggcgaa cgacgugcac     2880

auccugagca ucgaccgggg cgagcgccac cucgccuacu acacccuggu ggacgggaag     2940

ggcaacauca ucaagcagga caccuucaac aucaucggga acgaccgcau gaagaccaac     3000

uaccacgaca agcuggccgc caucgagaag gaccgggacu ccgcccgcaa ggacuggaag     3060

aagaucaaca acaucaagga gaugaaggag ggcuaccuca gccaggucgu gcacgagauc     3120

gcgaagcugg ugaucgagua caacgccauc gucguguucg aggaccugaa cuucggcuuc     3180

aagcgggggc gcuucaaggu ggagaagcag gucuaccaga agcucgagaa gaugcugauc     3240

gagaagcuga acuaccucgu guucaaggac aacgaguucg acaagacggg cggggugcug     3300

cgggccuacc agcugaccgc ccccuucgag accuucaaga agaugggcaa gcagaccggg     3360

aucaucuacu acguccccgc gggcuucacc uccaagaucu gccccgugac gggcuucgug     3420

aaccagcucu acccgaagua cgagagcguc uccaagagcc aggaguucuu cuccaaguuc     3480

gacaagaucu gcuacaaccu ggacaagggg uacuucgagu ucagcuucga cuacaagaac     3540

uucggcgaca aggccgccaa ggggaagugg accaucgccu ccuucggcag ccgccugauc     3600

aacuuccgga acuccgacaa gaaccacaac ugggacaccc gcgaggugua ccccaccaag     3660

gagcucgaga agcugcugaa ggacuacagc aucgaguacg gccacgggga gugcaucaag     3720

gccgcgaucu gcggcgaguc cgacaagaag uucuucgcca agcucacgag cgugcugaac     3780

accauccugc agaugcggaa cuccaagacc gggaccgagc ucgacuaccu gaucagcccc     3840

gucgccgacg ugaacggcaa cuucuucgac ucccgccagg cccccaagaa caugccgcag     3900

gacgccgacg cgaacggggc cuaccacauc ggccugaagg gccucaugcu gcuggggcgg     3960

aucaagaaca accaggaggg caagaagcuc aaccugguga ucaagaacga ggaguacuuc     4020

gaguucgucc agaaccgcaa caacaagcgg cccgccgcca cgaagaaggc cgggcaggcg     4080

aagaagaaga aguaaggacu aguguccacc ugucccuccu gggcugcugg auugucucgu     4140

uuuccugcca aauaaacagg aucagcgcuu uacagaucua aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  4248


<210>  2522
<211>  4433
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2522
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgga caagaaguac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccgugaucac cgacgaguac aaggucccca gcaagaaguu caaggugcug gggaacacgg      240

accggcacuc caucaagaag aaccugaucg gcgcccugcu guucgacagc ggcgagaccg      300

ccgaggccac ccgccugaag cggaccgcgc gccggcgcua cacgcggcgc aagaaccgga      360

ucugcuaccu gcaggagauc uucuccaacg agauggccaa gguggacgac agcuucuucc      420

accgccucga ggaguccuuc cuggucgagg aggacaagaa gcacgagcgg caccccaucu      480

ucgggaacau cguggacgag guggccuacc acgagaagua ccccaccauc uaccaccucc      540

gcaagaagcu cgucgacagc accgacaagg ccgaccugcg gcugaucuac cucgcgcugg      600

cccacaugau caaguuccgc ggccacuucc ugaucgaggg ggaccucaac ccggacaacu      660

ccgacgugga caagcuguuc auccagcugg ugcagaccua caaccagcug uucgaggaga      720

accccaucaa cgccagcggc gucgacgcca aggccauccu guccgcgcgg cugagcaagu      780

cccgccggcu cgagaaccug aucgcccagc ugcccgggga gaagaagaac ggccucuucg      840

gcaaccugau cgcccugagc cucgggcuga cgcccaacuu caaguccaac uucgaccugg      900

ccgaggacgc caagcuccag cugagcaagg acaccuacga cgacgaccug gacaaccucc      960

uggcgcagau cggcgaccag uacgccgacc uguuccucgc cgccaagaac cuguccgacg     1020

ccauccugcu cagcgacauc cugcgcguga acaccgagau caccaaggcg ccgcuguccg     1080

ccagcaugau caagcgguac gacgagcacc accaggaccu cacgcugcug aaggcccucg     1140

ugcgccagca gcugcccgag aaguacaagg agaucuucuu cgaccagagc aagaacgggu     1200

acgccggcua caucgacggc ggggccuccc aggaggaguu cuacaaguuc aucaagccca     1260

uccuggagaa gauggacggc accgaggagc uccuggucaa gcugaaccgg gaggaccugc     1320

uccgcaagca gcgcaccuuc gacaacggga gcauccccca ccagauccac cugggcgagc     1380

ugcacgcgau ccuccggcgc caggaggacu ucuacccguu ccugaaggac aaccgggaga     1440

agaucgagaa gauccugacc uuccgcaucc ccuacuacgu ggggccccuc gcccggggca     1500

acucccgcuu cgccuggaug acgcgcaaga gcgaggagac caucaccccc uggaacuucg     1560

aggagguggu cgacaagggc gccuccgcgc agagcuucau cgagcggaug accaacuucg     1620

acaagaaccu gccgaacgag aaggugcugc ccaagcacuc ccuccuguac gaguacuuca     1680

cgguguacaa cgagcugacc aaggucaagu acgugaccga ggggaugcgg aagcccgccu     1740

uccucagcgg cgagcagaag aaggccaucg uggaccugcu guucaagacc aaccgcaagg     1800

ucacggugaa gcagcucaag gaggacuacu ucaagaagau cgagugcuuc gacuccgucg     1860

agaucagcgg gguggaggac cgguucaacg ccucccuggg caccuaccac gaccugcuca     1920

agaucaucaa ggacaaggac uuccuggaca acgaggagaa cgaggacauc cuggaggaca     1980

ucgugcucac ccugacccug uucgaggacc gcgagaugau cgaggagcgg cucaagacgu     2040

acgcccaccu guucgacgac aaggucauga agcagcugaa gcgccggcgc uacaccgggu     2100

ggggccggcu cagccgcaag cugaucaacg gcauccggga caagcagucc gggaagacca     2160

uccuggacuu ccucaagagc gacggcuucg cgaaccgcaa cuucaugcag cugauccacg     2220

acgacucccu gaccuucaag gaggacaucc agaaggccca ggugagcggg cagggcgacu     2280

cccuccacga gcacaucgcc aaccuggccg ggagccccgc caucaagaag ggcauccugc     2340

agacggugaa ggucguggac gagcucguga aggucauggg ccgccacaag ccggagaaca     2400

ucgugaucga gauggcgcgg gagaaccaga ccacccagaa ggggcagaag aacucccgcg     2460

agcggaugaa gcgcaucgag gagggcauca aggagcuggg gagccagauc cugaaggagc     2520

accccgugga gaacacccag cuccagaacg agaagcugua ccuguacuac cuccagaacg     2580

gccgggacau guacgucgac caggagcugg acaucaaccg ccugagcgac uacgacgugg     2640

accacaucgu gccccagucc uuccucaagg acgacagcau cgacaacaag guccugacgc     2700

gguccgacaa gaaccgcggc aagagcgaca acgugcccuc cgaggaggug gucaagaaga     2760

ugaagaacua cuggcggcag cugcucaacg ccaagcugau cacccagcgc aaguucgaca     2820

accugaccaa ggccgagcgc gggggccuca gcgagcugga caaggccggg uucaucaagc     2880

ggcagcuggu ggagacccgg cagaucacca agcacguggc ccagauccuc gacucccgca     2940

ugaacacgaa guacgacgag aacgacaagc ugauccggga ggucaaggug aucacccuca     3000

agagcaagcu cguguccgac uuccgcaagg acuuccaguu cuacaagguc cgggagauca     3060

acaacuacca ccacgcgcac gacgccuacc ugaacgccgu ggugggcacc gcccugauca     3120

agaaguaccc gaagcucgag agcgaguucg ucuacgggga cuacaaggug uacgacgugc     3180

gcaagaugau cgcgaagucc gagcaggaga ucggcaaggc caccgccaag uacuucuucu     3240

acagcaacau caugaacuuc uucaagacgg agaucacccu ggccaacggc gagauccgga     3300

agcgcccccu gaucgagacc aacggggaga ccggcgagau cgucugggac aaggggcggg     3360

acuucgccac ggugcgcaag gugcucucca ugccccaggu caacaucgug aagaagaccg     3420

aggugcagac cggcggguuc agcaaggagu ccauccugcc caagcgcaac agcgacaagc     3480

ugaucgcgcg gaagaaggac ugggacccga agaaguacgg cggcuucgac ucccccaccg     3540

ucgccuacag cgugcucgug gucgccaagg uggagaaggg caagagcaag aagcugaagu     3600

ccgugaagga gcugcucggg aucacgauca uggagcgcag cuccuucgag aagaacccca     3660

ucgacuuccu ggaggccaag ggguacaagg aggucaagaa ggaccugauc aucaagcucc     3720

cgaaguacag ccuguucgag cuggagaacg gccggaagcg caugcucgcc uccgcggggg     3780

agcugcagaa gggcaacgag cuggcccucc ccagcaagua cgugaacuuc cuguaccugg     3840

ccucccacua cgagaagcuc aagggcagcc ccgaggacaa cgagcagaag cagcuguucg     3900

uggagcagca caagcacuac cuggacgaga ucaucgagca gaucuccgag uucagcaagc     3960

gggucauccu cgccgacgcc aaccuggaca aggugcuguc cgcguacaac aagcaccgcg     4020

acaagcccau ccgggagcag gccgagaaca ucauccaccu cuucacccug accaaccugg     4080

gggccccggc cgcguucaag uacuucgaca ccacgaucga ccgcaagcgg uacaccagca     4140

ccaaggaggu gcucgacgcc acccugaucc accaguccau cacgggccug uacgagaccc     4200

gcaucgaccu cagccagcuc gggggcgaca agcggcccgc cgccaccaag aaggccgggc     4260

aggcgaagaa gaagaaguaa ggacuagugu ccaccugucc cuccugggcu gcuggauugu     4320

cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4433


<210>  2523
<211>  5216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2523
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa cuucaagauc cugcccaucg ccaucgaccu cggcgugaag aacaccgggg      180

uguucagcgc cuucuaccag aagggcaccu cccuggagcg gcuggacaac aagaacggga      240

aggucuacga gcucagcaag gacuccuaca cgcugcugau gaacaaccgc accgcccggc      300

gccaccagcg gcgcggcauc gaccggaagc agcucgugaa gcgccuguuc aagcugaucu      360

ggaccgagca gcucaaccug gagugggaca aggacaccca gcaggccauc agcuuccugu      420

ucaaccggcg cggcuucucc uucaucacgg acggguacag ccccgaguac cucaacaucg      480

ugcccgagca ggucaaggcg auccugaugg acaucuucga cgacuacaac ggcgaggacg      540

accuggacuc cuaccucaag cuggccaccg agcaggagag caagaucucc gagaucuaca      600

acaagcugau gcagaagauc cucgaguuca agcugaugaa gcugugcacc gacaucaagg      660

acgacaaggu gagcaccaag acgcucaagg agaucaccuc cuacgaguuc gagcugcugg      720

ccgacuaccu cgccaacuac agcgaguccc ugaagaccca gaaguucagc uacaccgaca      780

agcaggggaa ccugaaggag cucuccuacu accaccacga caaguacaac auccaggagu      840

uccugaagcg gcacgcgacg aucaacgacc gcauccugga cacccuccug accgacgacc      900

uggacaucug gaacuucaac uucgagaagu ucgacuucga caagaacgag gagaagcucc      960

agaaccagga ggacaaggac cacauccagg cccaccucca ccacuucgug uucgccguca     1020

acaagaucaa gagcgagaug gccuccggcg ggcggcaccg cagccaguac uuccaggaga     1080

ucaccaacgu gcuggacgag aacaaccacc aggagggcua ccugaagaac uucugcgaga     1140

accugcacaa caagaaguac uccaaccuga gcgugaagaa ccucgucaac cugaucggca     1200

accugagcaa ccucgagcug aagccgcugc ggaaguacuu caacgacaag auccacgcca     1260

aggcggacca cugggacgag cagaaguuca cggagaccua cugccacugg auccucgggg     1320

aguggcgcgu gggcgugaag gaccaggaca agaaggacgg ggccaaguac uccuacaagg     1380

accugugcaa cgagcugaag cagaagguca ccaaggccgg ccucguggac uuccugcugg     1440

agcuggaccc cugccggacc aucccgcccu accucgacaa caacaaccgc aagccgccca     1500

agugccagag ccugauccug aaccccaagu uccucgacaa ccaguacccc aacuggcagc     1560

aguaccugca ggagcugaag aagcuccagu ccauccagaa cuaccuggac agcuucgaga     1620

cggaccugaa ggugcucaag uccagcaagg accagcccua cuucgucgag uacaagucca     1680

gcaaccagca gaucgccucc ggccagcggg acuacaagga ccuggacgcc cgcauccugc     1740

aguucaucuu cgaccgggug aaggcgagcg acgagcuccu gcugaacgag aucuacuucc     1800

aggccaagaa gcucaagcag aaggccucca gcgagcugga gaagcuggag uccagcaaga     1860

agcucgacga ggugaucgcc aacucccagc ugagccagau ccugaagucc cagcacacca     1920

acgggaucuu cgagcagggc accuuccucc accuggucug caaguacuac aagcagcgcc     1980

agcgcgcccg ggacagccgc cuguacauca ugcccgagua ccgguacgac aagaagcucc     2040

acaaguacaa caacaccggg cgcuucgacg acgacaacca gcugcugacg uacugcaacc     2100

acaagccccg gcagaagcgc uaccagcucc ugaacgaccu ggcgggcgug cuccaggugu     2160

cccccaacuu ccugaaggac aagaucggga gcgacgacga ccuguucauc agcaaguggc     2220

ucgucgagca cauccggggc uucaagaagg ccugcgagga cucccugaag auccagaagg     2280

acaaccgcgg ccugcucaac cacaagauca acaucgcccg gaacaccaag gggaagugcg     2340

agaaggagau cuucaaccug aucugcaaga ucgagggcag cgaggacaag aaggggaacu     2400

acaagcacgg ccuggccuac gagcucgggg ugcugcuguu cggcgagccg aacgaggccu     2460

ccaagcccga guucgaccgc aagaucaaga aguucaacag caucuacucc uucgcgcaga     2520

uccagcagau cgccuucgcc gagcggaagg gcaacgccaa cacgugcgcg gucugcagcg     2580

ccgacaacgc ccaccgcaug cagcagauca agaucaccga gcccguggag gacaacaagg     2640

acaagaucau ccucuccgcc aaggcccagc ggcugcccgc gaucccgacc cgcaucgugg     2700

acggggccgu caagaagaug gccaccaucc uggccaagaa caucguggac gacaacuggc     2760

agaacaucaa gcagguccuc agcgccaagc accagcugca cauccccauc aucaccgagu     2820

ccaacgcguu cgaguucgag cccgcccugg ccgacgugaa gggcaagagc cucaaggacc     2880

ggcgcaagaa ggcccuggag cggaucuccc ccgagaacau cuucaaggac aagaacaacc     2940

gcaucaagga guucgccaag gggaucagcg cguacuccgg cgccaaccug accgacgggg     3000

acuucgacgg cgccaaggag gagcucgacc acaucauccc gcggagccac aagaaguacg     3060

gcacgcugaa cgacgaggcc aaccugaucu gcgugacccg cggggacaac aagaacaagg     3120

gcaaccgcau cuucugccuc cgggaccugg cggacaacua caagcugaag caguucgaga     3180

ccaccgacga ccucgagauc gagaagaaga ucgccgacac gaucugggac gccaacaaga     3240

aggacuucaa guucgggaac uaccgcuccu ucaucaaccu gacgccccag gagcagaagg     3300

ccuuccggca cgcccuguuc cucgcggacg agaaccccau caagcaggcc gugauccgcg     3360

ccaucaacaa ccggaaccgc accuucguca acggcaccca gcgguacuuc gccgaggugc     3420

uggccaacaa caucuaccug cgcgcgaaga aggagaaccu caacaccgac aagaucagcu     3480

ucgacuacuu cggcaucccc accaucggga acggccgggg gaucgccgag auccgccagc     3540

uguacgagaa gguggacucc gacauccagg ccuacgccaa gggcgacaag ccgcaggcca     3600

gcuacagcca ccugaucgac gcgaugcucg ccuucugcau cgccgccgac gagcaccgga     3660

acgacggguc caucggccug gagaucgaca agaacuacag ccuguacccc cucgacaaga     3720

acaccggcga ggucuucacc aaggacaucu ucucccagau caagaucacc gacaacgagu     3780

ucagcgacaa gaagcuggug cgcaagaagg cgaucgaggg guucaacacg caccggcaga     3840

ugacccgcga cggcaucuac gccgagaacu accugcccau ccucauccac aaggagcuga     3900

acgaggugcg gaagggguac accuggaaga acuccgagga gaucaagauc uucaagggca     3960

agaaguacga cauccagcag cugaacaacc ucgucuacug ccugaaguuc guggacaagc     4020

cgaucagcau cgacauccag aucuccaccc uggaggagcu ccgcaacauc cugacgacca     4080

acaacaucgc cgccaccgcc gaguacuacu acaucaaccu gaagacccag aagcuccacg     4140

aguacuacau cgagaacuac aacacggcgc ugggguacaa gaaguacagc aaggagaugg     4200

aguuccugcg gucccucgcc uaccgcagcg agcgggugaa gaucaagucc aucgacgacg     4260

ucaagcaggu ccuggacaag gacagcaacu ucaucaucgg caagaucacc cugcccuuca     4320

agaaggagug gcagcgccuc uaccgggagu ggcagaacac caccaucaag gacgacuacg     4380

aguuccugaa guccuucuuc aacgucaaga gcaucacgaa gcugcacaag aaggugcgca     4440

aggacuucuc ccuccccauc agcaccaacg agggcaaguu ccuggugaag cgcaagaccu     4500

gggacaacaa cuucaucuac cagauccuga acgacuccga cagccgggcc gacgggacca     4560

agcccuucau cccggccuuc gacaucucca agaacgagau cguggaggcc aucaucgaca     4620

gcuucacgag caagaacauc uucuggcucc ccaagaacau cgagcugcag aagguggaca     4680

acaagaacau cuucgcgauc gacaccucca agugguucga gguggagacc cccagcgacc     4740

ugcgcgacau cggcaucgcc accauccagu acaagaucga caacaacucc cggcccaagg     4800

ugcgcgucaa gcuggacuac gugaucgacg acgacagcaa gaucaacuac uucaugaacc     4860

acucccuccu caagagccgg uacccggaca aggugcugga gauccugaag caguccacga     4920

ucaucgaguu cgagagcucc ggguucaaca agaccaucaa ggagaugcug ggcaugaagc     4980

uggccgggau cuacaacgag accagcaaca acaagcgccc cgccgccacc aagaaggcgg     5040

gccaggccaa gaagaagaag uaaggacuag uguccaccug ucccuccugg gcugcuggau     5100

ugucucguuu uccugccaaa uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa     5160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         5216


<210>  2524
<211>  3488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2524
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gcggaacuac auccugggcc ucgacaucgg gaucaccagc gugggcuacg      180

ggaucaucga cuacgagacc cgcgacguga ucgacgccgg cguccggcug uucaaggagg      240

ccaacgugga gaacaacgag ggccgccggu ccaagcgcgg ggcccggcgc cugaagcggc      300

gccggcgcca ccggauccag cgcgugaaga agcuccuguu cgacuacaac cugcucacgg      360

accacagcga gcuguccggc aucaaccccu acgaggcccg ggucaagggg cugagccaga      420

agcucuccga ggaggaguuc agcgcggccc ugcugcaccu cgccaagcgc cggggcgugc      480

acaacgugaa cgaggucgag gaggacaccg ggaacgagcu guccaccaag gagcagauca      540

gccgcaacuc caaggcccug gaggagaagu acguggcgga gcuccagcug gagcggcuga      600

agaaggacgg cgaggugcgc ggcagcauca accgguucaa gaccuccgac uacgucaagg      660

aggccaagca gcuccugaag gugcagaagg ccuaccacca gcuggaccag agcuucaucg      720

acacguacau cgaccuccug gagacccgcc gcaccuacua cgaggggccc ggcgaggggu      780

cccccuucgg cuggaaggac aucaaggagu gguacgagau gcugaugggc cacugcaccu      840

acuucccgga ggagcuccgg agcgugaagu acgccuacaa cgccgaccug uacaacgcgc      900

ugaacgaccu caacaaccug gucaucacgc gcgacgagaa cgagaagcug gaguacuacg      960

agaaguucca gaucaucgag aacguguuca agcagaagaa gaagcccacc cucaagcaga     1020

ucgccaagga gauccuggug aacgaggagg acaucaaggg guaccggguc accuccaccg     1080

gcaagcccga guucacgaac cugaaggugu accacgacau caaggacauc accgcccgca     1140

aggagaucau cgagaacgcc gagcuccugg accagaucgc caagauccug accaucuacc     1200

agagcuccga ggacauccag gaggagcuca ccaaccugaa cagcgagcug acgcaggagg     1260

agaucgagca gaucuccaac cucaaggggu acaccggcac ccacaaccug agccugaagg     1320

cgaucaaccu cauccuggac gagcuguggc acaccaacga caaccagauc gccaucuuca     1380

accggcucaa gcuggugccc aagaaggucg accugagcca gcagaaggag aucccgacga     1440

cccucgugga cgacuucauc cugucccccg uggucaagcg cagcuucauc caguccauca     1500

aggugaucaa cgccaucauc aagaaguacg ggcugcccaa cgacaucauc aucgagcugg     1560

cccgggagaa gaacagcaag gacgcccaga agaugaucaa cgagaugcag aagcgcaacc     1620

ggcagaccaa cgagcgcauc gaggagauca uccggaccac gggcaaggag aacgcgaagu     1680

accucaucga gaagaucaag cugcacgaca ugcaggaggg caagugccug uacucccucg     1740

aggccauccc ccuggaggac cugcucaaca acccguucaa cuacgagguc gaccacauca     1800

ucccccgcag cguguccuuc gacaacagcu ucaacaacaa ggugcugguc aagcaggagg     1860

agaacuccaa gaaggggaac cggacccccu uccaguaccu gagcuccagc gacuccaaga     1920

ucagcuacga gaccuucaag aagcacaucc ucaaccuggc caagggcaag gggcgcaucu     1980

ccaagaccaa gaaggaguac cugcucgagg agcgggacau caaccgcuuc agcgugcaga     2040

aggacuucau caaccggaac cugguggaca cgcgcuacgc cacccggggc cugaugaacc     2100

uccugcgcuc cuacuuccgc gucaacaacc uggacgugaa ggucaagagc aucaacgggg     2160

gcuucaccuc cuuccuccgg cgcaagugga aguucaagaa ggagcggaac aagggcuaca     2220

agcaccacgc cgaggacgcg cugaucaucg ccaacgccga cuucaucuuc aaggagugga     2280

agaagcugga caaggccaag aaggugaugg agaaccagau guucgaggag aagcaggcgg     2340

agagcaugcc cgagaucgag accgagcagg aguacaagga gaucuucauc acgccgcacc     2400

agaucaagca caucaaggac uucaaggacu acaaguacag ccaccgcgug gacaagaagc     2460

ccaaccggga gcucaucaac gacacccugu acuccacccg caaggacgac aaggggaaca     2520

cccugaucgu gaacaaccuc aacggccugu acgacaagga caacgacaag cugaagaagc     2580

ucaucaacaa gagccccgag aagcugcuga uguaccacca cgacccccag acguaccaga     2640

agcucaagcu gaucauggag caguacgggg acgagaagaa cccgcuguac aaguacuacg     2700

aggagaccgg caacuaccuc accaaguacu ccaagaagga caacgggccc gucaucaaga     2760

agaucaagua cuacggcaac aagcugaacg cccaccugga caucaccgac gacuacccca     2820

acagccggaa caagguggug aagcucuccc ugaagcccua ccgcuucgac gucuaccugg     2880

acaacggcgu guacaaguuc gugaccguca agaaccucga cgugaucaag aaggagaacu     2940

acuacgaggu gaacagcaag ugcuacgagg aggccaagaa gcugaagaag aucuccaacc     3000

aggccgaguu caucgccagc uucuacaaca acgaccugau caagaucaac ggggagcucu     3060

accgggucau cggcgugaac aacgaccugc ugaaccgcau cgaggugaac augaucgaca     3120

ucacguaccg ggaguaccuc gagaacauga acgacaagcg cccgccccgg aucaucaaga     3180

ccaucgcguc caagacccag agcaucaaga aguacuccac cgacauccug gggaaccugu     3240

acgaggucaa gagcaagaag cacccccaga ucaucaagaa gggcaagcgc cccgccgcca     3300

cgaagaaggc cggccaggcc aagaagaaga aguaaggacu aguguccacc ugucccuccu     3360

gggcugcugg auugucucgu uuuccugcca aauaaacagg aucagcgcuu uacagaucua     3420

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaagaauu                                                              3488


<210>  2525
<211>  4556
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2525
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgcu guucaacaag ugcaucauca ucagcaucaa ccucgacuuc uccaacaagg      180

agaagugcau gaccaagccc uacagcaucg gccuggacau cgggaccaac uccgugggcu      240

gggccgugau cacggacaac uacaaggucc ccagcaagaa gaugaaggug cuggggaaca      300

ccuccaagaa guacaucaag aagaaccucc ugggcgugcu gcuguucgac agcggcauca      360

ccgccgaggg gcggcgccug aagcggaccg cccgccggcg cuacacgcgg cgccggaacc      420

gcauccugua ccuccaggag aucuucucca ccgagauggc cacccuggac gacgcguucu      480

uccagcggcu ggacgacagc uuccucgucc ccgacgacaa gcgcgacucc aaguacccga      540

ucuucggcaa ccugguggag gagaaggugu accacgacga guuccccacc aucuaccacc      600

uccggaagua ccucgccgac agcacgaaga aggccgaccu gcgccugguc uaccucgccc      660

uggcgcacau gaucaaguac cgggggcacu uccugaucga gggcgaguuc aacuccaaga      720

acaacgacau ccagaagaac uuccaggacu uccucgacac cuacaacgcc aucuucgaga      780

gcgaccuguc ccuggagaac agcaagcagc ucgaggagau cgugaaggac aagaucucca      840

agcuggagaa gaaggaccgc auccugaagc uguuccccgg ggagaagaac agcggcaucu      900

ucuccgaguu ccugaagcug aucgugggca accaggccga cuuccggaag ugcuucaacc      960

ucgacgagaa ggccagccug cacuucucca aggagagcua cgacgaggac cuggagaccc     1020

uccuggggua caucggcgac gacuacagcg acgucuuccu gaaggccaag aagcucuacg     1080

acgcgauccu gcuguccggg uuccucaccg ugacggacaa cgagaccgag gcgccccuga     1140

gcuccgccau gaucaagcgc uacaacgagc acaaggagga ccuggcccuc cugaaggagu     1200

acauccggaa caucagccug aagaccuaca acgagguguu caaggacgac accaagaacg     1260

gcuacgccgg cuacaucgac gggaagacga accaggagga cuucuacguc uaccucaaga     1320

accugcuggc cgaguucgag ggcgccgacu acuuccucga gaagaucgac cgcgaggacu     1380

uccugcggaa gcagcgcacc uucgacaacg gguccauccc guaccagauc caccuccagg     1440

agaugcgcgc cauccuggac aagcaggcca aguucuaccc cuuccucgcc aagaacaagg     1500

agcggaucga gaagauccug accuuccgca uccccuacua cgugggcccc cuggcgcggg     1560

ggaacagcga cuucgccugg uccauccgca agcggaacga gaagaucacc ccguggaacu     1620

ucgaggacgu gaucgacaag gagagcuccg ccgaggccuu caucaaccgc augacgagcu     1680

ucgaccucua ccugcccgag gagaaggucc ugcccaagca cucccuccug uacgagaccu     1740

ucaacgugua caacgagcug accaaggugc gguucaucgc cgagagcaug cgcgacuacc     1800

aguuccucga cuccaagcag aagaaggaca ucguccggcu guacuucaag gacaagcgca     1860

aggugaccga caaggacauc aucgaguacc ugcacgcgau cuacggcuac gacggcaucg     1920

agcucaaggg gaucgagaag caguucaaca gcucccugag cacguaccac gaccugcuca     1980

acaucaucaa cgacaaggag uuccuggacg acuccagcaa cgaggccauc aucgaggaga     2040

ucauccacac ccugaccauc uucgaggacc gggagaugau caagcagcgc cucuccaagu     2100

ucgagaacau cuucgacaag agcgugcuga agaagcugag ccggcgccac uacaccggcu     2160

gggggaagcu cuccgccaag cugaucaacg gcauccggga cgagaagucc gggaacacga     2220

uccuggacua ccucaucgac gacggcauca gcaaccgcaa cuucaugcag cugauccacg     2280

acgacgcccu gagcuucaag aagaagaucc agaaggcgca gaucaucggc gacgaggaca     2340

aggggaacau caaggagguc gugaaguccc uccccggcag cccggccauc aagaagggga     2400

uccugcaguc caucaagauc gucgacgagc uggugaaggu gaugggcggg cggaagcccg     2460

agagcaucgu cguggagaug gcccgcgaga accaguacac caaccagggc aaguccaaca     2520

gccagcagcg gcucaagcgc cuggagaagu cccugaagga gcucggcagc aagauccuga     2580

aggagaacau ccccgccaag cuguccaaga ucgacaacaa cgcccuccag aacgaccgcc     2640

uguaccugua cuaccuccag aacgggaagg acauguacac cggcgacgac cuggacaucg     2700

accggcugag caacuacgac aucgaccaca ucauccccca ggcguuccuc aaggacaacu     2760

ccaucgacaa caaggugcug gucagcuccg ccagcaaccg cgggaagucc gacgacuucc     2820

cgagccugga gguggugaag aagcggaaga ccuucuggua ccagcuccuc aagagcaagc     2880

ugaucuccca gcgcaaguuc gacaaccuga cgaaggccga gcggggcggc cugcugcccg     2940

aggacaaggc cggguucauc cagcgccagc ucgucgagac ccggcagauc accaagcacg     3000

uggcccgccu gcuggacgag aaguucaaca acaagaagga cgagaacaac cgggcggugc     3060

gcaccgucaa gaucaucacg cucaagagca cccugguguc ccaguuccgg aaggacuucg     3120

agcuguacaa ggugcgcgag aucaacgacu uccaccacgc ccacgacgcc uaccucaacg     3180

ccgucaucgc cagcgcgcug cugaagaagu accccaagcu cgagcccgag uucguguacg     3240

gcgacuaccc gaaguacaac uccuuccggg agcgcaagag cgccaccgag aagguguacu     3300

ucuacuccaa caucaugaac aucuucaaga aguccaucag ccuggccgac gggcggguca     3360

ucgagcgccc ccugaucgag gugaacgagg agaccggcga gagcgugugg aacaaggagu     3420

ccgaccucgc caccguccgg cgcgugcuga gcuaccccca ggugaacguc gugaagaagg     3480

uggaggagca gaaccacggg cuggaccggg gcaagcccaa gggccucuuc aacgcgaacc     3540

uguccagcaa gccgaagccc aacuccaacg agaaccuggu cggggccaag gaguaccucg     3600

accccaagaa guacggcggg uacgccggca ucagcaacuc cuucgccgug cuggugaagg     3660

ggacgaucga gaagggcgcc aagaagaaga ucaccaacgu ccuggaguuc cagggcauca     3720

gcauccucga ccgcaucaac uaccggaagg acaagcugaa cuuccugcuc gagaaggggu     3780

acaaggacau cgagcugauc aucgagcugc cgaaguacag ccucuucgag cuguccgacg     3840

gcagccgccg caugcuggcg uccauccuca gcaccaacaa caagcgcggg gagauccaca     3900

agggcaacca gaucuuccug ucccagaagu ucgugaagcu gcucuaccac gccaagcgga     3960

ucagcaacac caucaacgag aaccaccgga aguacgugga gaaccacaag aaggaguucg     4020

aggagcuguu cuacuacauc cuggaguuca acgagaacua cgucggggcc aagaagaacg     4080

gcaagcuccu gaacuccgcc uuccagagcu ggcagaacca cuccaucgac gagcugugca     4140

gcuccuucau cggccccacg gggagcgagc gcaagggccu cuucgagcug accucccggg     4200

ggagcgccgc ggacuucgag uuccugggcg ugaagauccc ccgcuaccgg gacuacaccc     4260

ccuccagccu ccugaaggac gccacccuga uccaccaguc cgugacgggg cuguacgaga     4320

cccgcaucga ccucgccaag cugggcgagg gcaagcggcc ggccgccacc aagaaggcgg     4380

ggcaggccaa gaagaagaag uaaggacuag uguccaccug ucccuccugg gcugcuggau     4440

ugucucguuu uccugccaaa uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         4556


<210>  2526
<211>  3632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2526
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgug guacgccagc cugauguccg cccaccaccu ccgggugggc aucgacgugg      180

ggacccacag cgucggccug gccacccugc gcguggacga ccacgggacg cccaucgagc      240

uccuguccgc ccugagccac auccacgacu ccggcguggg caaggagggg aagaaggacc      300

acgacacccg gaagaagcuc agcggcaucg cgcgccgggc ccgccggcug cugcaccacc      360

gccggaccca gcuccagcag cuggacgagg uccugcgcga ccucggguuc cccaucccca      420

ccccgggcga guuccuggac cugaacgagc agacggaccc cuaccgggug uggcgcgugc      480

gggcccgccu cgucgaggag aagcugcccg aggagcugcg ggggcccgcc aucuccaugg      540

cggugcgcca caucgcccgg caccgcggcu ggcggaaccc guacagcaag guggaguccc      600

uccugagccc cgccgaggag ucccccuuca ugaaggcccu gcgcgagcgg auccucgcca      660

ccaccggcga gguccuggac gacgggauca cccccggcca ggcgauggcc cagguggccc      720

ugacgcacaa caucagcaug cgcgggccgg agggcauccu cggcaagcug caccaguccg      780

acaacgccaa cgagauccgc aagaucugcg cccggcaggg ggugagcccc gacgucugca      840

agcagcugcu ccgcgcggug uucaaggccg acagcccccg gggcuccgcc gugucccgcg      900

ucgcccccga cccgcugccc gggcagggca gcuuccggcg cgcccccaag ugcgaccccg      960

aguuccagcg guuccgcauc aucuccaucg uggcgaaccu gcggaucagc gagaccaagg     1020

gggagaaccg cccgcucacc gccgacgagc ggcgccacgu ggucaccuuc cugacggagg     1080

acagccaggc cgaccugacc uggguggacg uggccgagaa gcucggcguc caccggcgcg     1140

accugcgggg caccgccgug cacaccgacg acggggagcg cuccgcggcc cggccgccca     1200

ucgacgccac ggaccgcauc augcggcaga ccaagaucag cucccugaag accugguggg     1260

aggaggccga cagcgagcag cgcggcgcga ugauccgcua ccucuacgag gaccccaccg     1320

acuccgagug cgccgagauc aucgccgagc ugcccgagga ggaccaggcc aagcuggaca     1380

gccuccaccu ccccgccggg cgggcggccu acucccgcga gagccugacg gcccuguccg     1440

accacaugcu ggccaccacc gacgaccugc acgaggcccg gaagcgccuc uucggcgucg     1500

acgacagcug ggcgccgccc gccgaggcca ucaacgcccc cguggggaac cccuccgugg     1560

accggacccu gaagaucguc ggccgcuacc ugagcgccgu ggaguccaug uggggcacgc     1620

ccgaggugau ccacgucgag cacgugcggg acggguucac cagcgagcgc auggcggacg     1680

agcgggacaa ggccaaccgc cggcgcuaca acgacaacca ggaggccaug aagaagaucc     1740

agcgggacua cggcaaggag ggguacaucu cccgcggcga caucgugcgg cucgacgccc     1800

uggagcugca ggggugcgcg ugccuguacu gcggcaccac caucggcuac cacacgugcc     1860

agcucgacca caucgucccg caggccgggc ccggcagcaa caaccgccgg gggaaccugg     1920

uggccgugug cgagcgcugc aaccggucca agagcaacac ccccuucgcc gucugggccc     1980

agaagugcgg caucccccac gugggcguga aggaggcgau cgggcgcguc cggggcuggc     2040

gcaagcagac cccgaacacc uccagcgagg accugacgcg ccucaagaag gaggugaucg     2100

cccggcugcg ccggacccag gaggaccccg agaucgacga gcgcagcaug gaguccgugg     2160

ccuggauggc caacgagcug caccaccgga ucgccgcggc cuaccccgag accaccguca     2220

ugguguaccg cgggagcauc acggccgccg cccggaaggc ggccggcauc gacucccgca     2280

ucaaccucau cggggagaag ggccggaagg accgcaucga ccggcgccac cacgccgugg     2340

acgccagcgu cguggcgcug auggaggccu ccguggccaa gacccuggcc gagcggagcu     2400

cccuccgcgg cgagcagcgg cugaccggga aggagcagac cuggaagcag uacaccggca     2460

gcacggucgg ggcccgcgag cacuucgaga uguggcgggg ccacaugcug caccucaccg     2520

agcuguucaa cgagcgccug gcggaggaca agguguacgu gacccagaac auccggcucc     2580

gccuguccga cgggaacgcc cacaccguca accccagcaa gcuggugucc caccgccucg     2640

gcgacggccu gacggugcag cagaucgacc gggccugcac cccggcccug uggugcgccc     2700

ucacccgcga gaaggacuuc gacgagaaga acgggcugcc cgcgcgggag gaccgcgcca     2760

uccgggucca cggccacgag aucaagagcu ccgacuacau ccagguguuc agcaagcgca     2820

agaagaccga cuccgaccgg gacgagacgc ccuucggggc caucgccgug cgcggcgggu     2880

ucgucgagau cggccccagc auccaccacg cccggaucua ccgcguggag ggcaagaagc     2940

cgguguacgc gaugcugcgg gucuucaccc acgaccuccu gucccagcgc cacggggacc     3000

uguucagcgc cgugaucccg ccccagagca ucuccaugcg gugcgccgag cccaagcucc     3060

gcaaggccau caccaccggc aacgccacgu accuggggug gguggucgug ggcgacgagc     3120

uggagaucaa cguggacagc uucaccaagu acgcgaucgg gcgguuccuc gaggacuucc     3180

ccaacaccac ccgcuggcgg aucugcggcu acgacacgaa cuccaagcug acccugaagc     3240

ccaucguccu cgccgccgag ggccuggaga accccagcuc cgccgugaac gagaucgugg     3300

agcugaaggg guggcgcguc gcgaucaacg ugcucaccaa ggugcacccc accgucgugc     3360

ggcgcgacgc ccugggccgg ccgcgcuaca gcucccgcag caaccugccc acguccugga     3420

ccaucgagaa gcggcccgcc gccaccaaga aggccgggca ggcgaagaag aagaaguaag     3480

gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu gccaaauaaa     3540

caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3632


<210>  2527
<211>  4493
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2527
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgac caagcccuac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccgugacgac cgacaacuac aaggucccca gcaagaagau gaaggugcuc gggaacaccu      240

ccaagaagua caucaagaag aaccugcugg gcgugcuccu guucgacagc ggcaucaccg      300

ccgaggggcg gcgccugaag cggacggccc gccggcgcua cacccggcgc cggaaccgca      360

uccucuaccu gcaggagauc uucuccaccg agauggccac ccuggacgac gcguucuucc      420

agcggcucga cgacagcuuc cugguccccg acgacaagcg cgacuccaag uacccgaucu      480

ucggcaaccu gguggaggag aaggccuacc acgacgaguu ccccacgauc uaccaccucc      540

ggaaguaccu ggccgacagc accaagaagg ccgaccugcg ccucguguac cuggcgcugg      600

cccacaugau caaguaccgg gggcacuucc ucaucgaggg cgaguucaac uccaagaaca      660

acgacaucca gaagaacuuc caggacuucc uggacaccua caacgccauc uucgagagcg      720

accugucccu cgagaacagc aagcagcugg aggagaucgu caaggacaag aucuccaagc      780

uggagaagaa ggaccgcauc cucaagcugu uccccgggga gaagaacagc ggcaucuucu      840

ccgaguuccu gaagcucauc gugggcaacc aggccgacuu ccggaagugc uucaaccugg      900

acgagaaggc cagccugcac uucuccaagg agagcuacga cgaggaccuc gagacccugc      960

ugggguacau cggcgacgac uacuccgacg uguuccucaa ggcgaagaag cuguacgacg     1020

ccauccugcu cagcggguuc cugacgguca ccgacaacga gaccgaggcg ccccugagcu     1080

ccgccaugau caagcgcuac aacgagcaca aggaggaccu cgcccugcug aaggaguaca     1140

uccggaacau cagccucaag accuacaacg agguguucaa ggacgacacg aagaacggcu     1200

acgccggcua caucgacggg aagaccaacc aggaggacuu cuacguguac cugaagaagc     1260

ugcucgccga guucgagggc gccgacuacu uccuggagaa gaucgaccgc gaggacuucc     1320

ugcggaagca gcgcaccuuc gacaacgggu ccaucccgua ccagauccac cuccaggaga     1380

ugcgcgccau ccuggacaag caggccaagu ucuaccccuu ccuggcgaag aacaaggagc     1440

ggaucgagaa gauccugacc uuccgcaucc ccuacuacgu cggcccccuc gcccggggga     1500

acagcgacuu cgccuggucc auccgcaagc ggaacgagaa gaucacgccg uggaacuucg     1560

aggacgugau cgacaaggag agcuccgccg aggccuucau caaccgcaug accagcuucg     1620

accuguaccu gcccgaggag aaggugcucc ccaagcacuc ccugcuguac gagaccuuca     1680

acgucuacaa cgagcucacc aaggugcggu ucaucgcgga gagcaugcgc gacuaccagu     1740

uccuggacuc caagcagaag aaggacaucg ugcggcugua cuucaaggac aagcgcaagg     1800

ucacggacaa ggacaucauc gaguaccucc acgccaucua cggcuacgac ggcaucgagc     1860

ugaaggggau cgagaagcag uucaacagcu cccugagcac cuaccacgac cuccugaaca     1920

ucaucaacga caaggaguuc cuggacgacu ccagcaacga ggccaucauc gaggagauca     1980

uccacacccu caccaucuuc gaggaccggg agaugaucaa gcagcgccug uccaaguucg     2040

agaacaucuu cgacaagagc gugcugaaga agcucucccg gcgccacuac acgggcuggg     2100

ggaagcugag cgccaagcug aucaacggca uccgggacga gaaguccggg aacaccaucc     2160

ucgacuaccu gaucgacgac ggcaucagca accgcaacuu caugcagcug auccacgacg     2220

acgcgcucag cuucaagaag aagauccaga aggcccagau caucggcgac gaggacaagg     2280

ggaacaucaa ggaggugguc aagucccugc ccggcagccc ggccaucaag aaggggaucc     2340

ugcaguccau caagaucgug gacgagcucg ucaaggugau gggcgggcgg aagcccgaga     2400

gcaucguggu cgagauggcc cgcgagaacc aguacaccaa ccagggcaag uccaacagcc     2460

agcagcggcu gaagcgccug gagaaguccc ucaaggagcu gggcagcaag auccugaagg     2520

agaacauccc cgccaagcuc uccaagaucg acaacaacgc gcugcagaac gaccgccugu     2580

accucuacua ccugcagaac gggaaggaca uguacaccgg cgacgaccug gacaucgacc     2640

ggcucagcaa cuacgacauc gaccacauca ucccccaggc cuuccugaag gacaacucca     2700

ucgacaacaa ggugcuggug agcuccgcca gcaaccgcgg gaaguccgac gacgucccga     2760

gccucgaggu ggugaagaag cggaagacgu ucugguacca gcugcucaag agcaagcuga     2820

ucucccagcg caaguucgac aaccugacca aggccgagcg gggcggccug agccccgagg     2880

acaaggccgg guucauccag cgccagcucg ucgagacccg gcagaucacc aagcacgugg     2940

cgcgccugcu ggacgagaag uucaacaaca agaaggacga gaacaaccgg gccgugcgca     3000

cggucaagau caucacccuc aaguccaccc uggugagcca guuccggaag gacuucgagc     3060

uguacaaggu gcgcgagauc aacgacuucc accacgccca cgacgccuac cucaacgccg     3120

ucguggcguc cgcccugcug aagaaguacc ccaagcucga gcccgaguuc guguacggcg     3180

acuacccgaa guacaacagc uuccgggagc gcaaguccgc caccgagaag gucuacuucu     3240

acagcaacau caugaacauc uucaagaagu ccaucagccu ggccgacggg cgggugaucg     3300

agcgcccccu gaucgaggug aacgaggaga ccggcgaguc cgucuggaac aaggagagcg     3360

accucgcgac ggugcggcgc gugcuguccu acccccaggu caacguggug aagaaggucg     3420

aggagcagaa ccacgggcug gaccggggca agcccaaggg ccucuucaac gccaaccuga     3480

gcuccaagcc gaagcccaac agcaacgaga accugguggg ggccaaggag uaccucgacc     3540

ccaagaagua cggcggguac gccggcaucu ccaacagcuu caccgugcug gucaagggga     3600

ccaucgagaa gggcgccaag aagaagauca ccaacgugcu ggaguuccag ggcaucucca     3660

uccucgaccg caucaacuac cggaaggaca agcugaacuu ccugcucgag aagggguaca     3720

aggacaucga gcugaucauc gagcugccga aguacagccu cuucgagcug agcgacggcu     3780

cccgccgcau gcuggcgagc auccucucca cgaacaacaa gcgcggggag auccacaagg     3840

gcaaccagau cuuccugagc cagaaguucg ugaagcugcu cuaccacgcc aagcggaucu     3900

ccaacaccau caacgagaac caccggaagu acgucgagaa ccacaagaag gaguucgagg     3960

agcuguucua cuacauccug gaguucaacg agaacuacgu gggggccaag aagaacggca     4020

agcuccugaa cagcgccuuc caguccuggc agaaccacag caucgacgag cugugcucca     4080

gcuucaucgg ccccaccggg uccgagcgca agggccucuu cgagcugacc agccgggggu     4140

ccgccgcgga cuucgaguuc cugggcguga agaucccccg cuaccgggac uacacgccca     4200

gcucccuccu gaaggacgcc acccugaucc accagagcgu caccgggcuc uacgagaccc     4260

gcaucgaccu ggccaagcug ggcgagggca agcggccggc cgccacgaag aaggcggggc     4320

aggccaagaa gaagaaguaa ggacuagugu ccaccugucc cuccugggcu gcuggauugu     4380

cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4493


<210>  2528
<211>  3575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2528
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc cgccuucaag cccaacagca ucaacuacau ccugggccuc gacaucggga      180

ucgccuccgu gggcugggcc augguggaga ucgacgagga ggagaacccc auccggcuga      240

ucgaccuggg gguccgcgug uucgagcggg cggaggugcc caagaccggc gacagccucg      300

ccauggcccg ccggcuggcc cgcuccgucc ggcgccugac ccggcgccgg gcgcaccgcc      360

uccugcggac gcgccggcug cucaagcgcg agggcgugcu gcaggccgcc aacuucgacg      420

agaacgggcu gaucaagagc cucccgaaca cccccuggca gcugcgggcc gccgcgcugg      480

accgcaagcu cacgccccug gagugguccg ccgugcugcu ccaccugauc aagcaccggg      540

gcuaccugag ccagcgcaag aacgaggggg agaccgccga caaggagcuc ggcgcccugc      600

ugaagggggu cgccggcaac gcgcacgccc uccagaccgg cgacuuccgg acccccgccg      660

agcuggcccu gaacaaguuc gagaaggagu ccgggcacau ccgcaaccag cgcagcgacu      720

acucccacac cuucagccgg aaggaccucc aggccgagcu gauccuccug uucgagaagc      780

agaaggaguu cggcaacccg cacguguccg ggggccugaa ggagggcauc gagacccugc      840

ucaugacgca gcgccccgcg cugagcgggg acgccgugca gaagaugcug ggccacugca      900

ccuucgagcc cgccgagccc aaggccgcca agaacaccua caccgcggag cgguucaucu      960

ggcucacgaa gcugaacaac cugcgcaucc ucgagcaggg guccgagcgg ccgcugaccg     1020

acaccgagcg cgccacccug auggacgagc ccuaccgcaa gagcaagcuc acguacgccc     1080

aggcccggaa gcugcugggc cucgaggaca ccgcguucuu caaggggcug cgguacggca     1140

aggacaacgc cgaggccucc acccugaugg agaugaaggc cuaccacgcc aucagccgcg     1200

cgcucgagaa ggagggccug aaggacaaga aguccccgcu gaaccugagc cccgagcucc     1260

aggacgagau cgggaccgcc uucucccugu ucaagacgga cgaggacauc accggccggc     1320

ugaaggaccg cauccagccg gagauccucg aggcccugcu gaagcacauc agcuucgaca     1380

aguucgucca gaucagccuc aaggcccugc ggcgcaucgu gccccugaug gagcagggga     1440

agcgguacga cgaggccugc gcggagaucu acggcgacca cuacgggaag aagaacaccg     1500

aggagaagau cuaccucccg cccauccccg ccgacgagau ccgcaacccc gugguccugc     1560

gggcccuguc ccaggcccgc aaggugauca acggcguggu ccggcgcuac ggcagccccg     1620

cccgcaucca caucgagacc gcgcgggagg uggggaaguc cuucaaggac cgcaaggaga     1680

ucgagaagcg gcaggaggag aaccgcaagg accgggagaa ggccgccgcc aaguuccgcg     1740

aguacuuccc caacuucgug ggcgagccca agagcaagga cauccucaag cugcggcugu     1800

acgagcagca gcacgggaag ugccucuacu ccggcaagga gaucaaccug gggcgccuga     1860

acgagaaggg cuacgucgag aucgaccacg cgcuccccuu cagccggacg ugggacgacu     1920

ccuucaacaa caaggugcug gugcugggca gcgagaacca gaacaagggg aaccagaccc     1980

ccuacgagua cuucaacggc aaggacaacu cccgcgagug gcaggaguuc aaggcccggg     2040

ucgagaccag ccgcuucccc cgguccaaga agcagcgcau ccuccugcag aaguucgacg     2100

aggacggguu caaggagcgg aaccugaacg acacccgcua cgugaaccgg uuccucugcc     2160

aguucgucgc cgaccgcaug cggcugacgg gcaagggcaa gaagcgcgug uucgccagca     2220

acgggcagau caccaaccug cuccggggcu ucugggggcu gcgcaaggug cgcgccgaga     2280

acgaccggca ccacgcgcug gacgccgucg ugguggccug cuccaccguc gccaugcagc     2340

agaagaucac ccgcuucgug cgguacaagg agaugaacgc cuucgacggc aagacgaucg     2400

acaaggagac cggggaggug cuccaccaga agacccacuu cccgcagccc ugggaguucu     2460

ucgcgcagga ggucaugauc cgcguguucg gcaagcccga cggcaagccg gaguucgagg     2520

aggccgacac ccuggagaag cugcggacgc uccuggccga gaagcugagc ucccgccccg     2580

aggccgugca cgaguacguc acgccccucu ucgugagccg ggcccccaac cgcaagaugu     2640

ccgggcaggg ccacauggag accgucaaga gcgcgaagcg gcuggacgag gggguguccg     2700

ugcugcgcgu gccgcucacc cagcugaagc ugaaggaccu cgagaagaug gucaaccggg     2760

agcgcgagcc caagcuguac gaggcccuga aggcccggcu cgaggcccac aaggacgacc     2820

ccgcgaaggc cuucgccgag cccuucuaca aguacgacaa ggccggcaac cgcacccagc     2880

aggucaaggc cgugcgggug gagcaggucc agaagaccgg ggugugggug cgcaaccaca     2940

acggcaucgc ggacaacgcc accauggugc ggguggacgu cuucgagaag ggcgacaagu     3000

acuaccuggu gccgaucuac agcuggcagg ucgccaaggg gauccugccc gaccgcgccg     3060

uggugcaggg caaggacgag gaggacuggc agcucaucga cgacagcuuc aacuucaagu     3120

ucucccugca ccccaacgac cugguggagg ucaucaccaa gaaggcccgg auguucgggu     3180

acuucgcgag cugccaccgc ggcaccggga acaucaacau ccgcauccac gaccucgacc     3240

acaagaucgg caagaacggc auccuggagg ggaucggcgu gaagacggcc cuguccuucc     3300

agaaguacca gaucgacgag cucgggaagg agauccggcc cugccgccug aagaagcggc     3360

cgcccgugcg caagcggccc gccgccacca agaaggccgg ccaggcgaag aagaagaagu     3420

aaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu ccugccaaau     3480

aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                3575


<210>  2529
<211>  4331
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2529
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gaagcccuac accaucggcc uggacaucgg gaccaacagc gugggcuggg      180

ccgugcucac ggaccaguac gaccugguca agcggaagau gaagaucgcc ggggacuccg      240

agaagaagca gaucaagaag aacuucuggg gcgugcgccu guucgacgag ggccagaccg      300

ccgccgaccg gcgcauggcg cggaccgccc gccggcgcau cgagcggcgc cggaaccgca      360

ucagcuaccu ccaggggauc uucgccgagg agauguccaa gaccgacgcc aacuucuucu      420

gccggcugag cgacuccuuc uacguggaca acgagaagcg caacagccgg caccccuucu      480

ucgcgacgau cgaggaggag gucgaguacc acaagaacua ccccaccauc uaccaccucc      540

gcgaggagcu ggugaacucc agcgagaagg ccgaccugcg gcugguguac cucgcccugg      600

cccacaucau caaguaccgc ggcaacuucc ugaucgaggg ggcccucgac acccagaaca      660

ccuccgucga cggcaucuac aagcaguuca uccagacgua caaccaggug uucgcgagcg      720

ggaucgagga cggcucccug aagaagcugg aggacaacaa ggacguggcc aagauccucg      780

ucgagaaggu gacccggaag gagaagcugg agcgcauccu gaagcucuac ccgggcgaga      840

aguccgccgg gauguucgcc caguucauca gccugaucgu gggcagcaag gggaacuucc      900

agaagcccuu cgaccugauc gagaaguccg acaucgagug cgccaaggac agcuacgagg      960

aggaccucga gucccugcug gcgcucaucg gcgacgagua cgccgagcug uucgucgccg     1020

ccaagaacgc cuacagcgcg guggugcugu ccagcaucau caccgucgcc gagaccgaga     1080

cgaacgccaa gcucagcgcc uccaugaucg agcgguucga cacccacgag gaggaccugg     1140

gcgagcugaa ggccuucauc aagcuccacc uccccaagca cuacgaggag aucuucagca     1200

acaccgagaa gcacggguac gcgggcuaca ucgacgggaa gaccaagcag gccgacuucu     1260

acaaguacau gaagaugacg cuggagaaca ucgagggcgc cgacuacuuc aucgccaaga     1320

ucgagaagga gaacuuccug cgcaagcagc gcaccuucga caacggggcg aucccccacc     1380

agcugcaccu ggaggagcuc gaggccaucc ugcaccagca ggccaaguac uacccguucc     1440

ugaaggagaa cuacgacaag aucaaguccc ucgugaccuu ccggaucccc uacuucgugg     1500

gcccccuggc caacggccag agcgaguucg ccuggcugac ccgcaaggcg gacggggaga     1560

uccggcccug gaacaucgag gagaaggucg acuucggcaa guccgccgug gacuucaucg     1620

agaagaugac gaacaaggac accuaccucc cgaaggagaa cgugcugccc aagcacagcc     1680

ugugcuacca gaaguaccuc gucuacaacg agcugaccaa ggugcgcuac aucaacgacc     1740

aggggaagac cuccuacuuc agcggccagg agaaggagca gaucuucaac gaccuguuca     1800

agcagaagcg gaaggucaag aagaaggacc ucgagcuguu ccugcgcaac augucccacg     1860

uggagagccc cacgaucgag gggcuggagg acuccuucaa cagcuccuac agcaccuacc     1920

acgaccuccu gaaggugggc aucaagcagg agauccugga caaccccguc aacaccgaga     1980

ugcucgagaa caucgugaag auccugaccg uguucgagga caagcggaug aucaaggagc     2040

agcugcagca guucuccgac guccucgacg gcguggugcu gaagaagcug gagcgccggc     2100

acuacacggg guggggccgc cucagcgcca agcugcugau ggggauccgg gacaagcagu     2160

cccaccucac cauccuggac uaccugauga acgacgacgg ccucaaccgc aaccugaugc     2220

agcugaucaa cgacagcaac cucuccuuca agagcaucau cgagaaggag caggucacca     2280

ccgccgacaa ggacauccag agcaucgugg ccgaccuggc gggguccccg gccaucaaga     2340

agggcauccu gcagagccuc aagaucgugg acgagcuggu cuccgugaug ggcuacccgc     2400

cccagacgau cguggucgag auggcccggg agaaccagac caccgggaag ggcaagaaca     2460

acagccgccc ccgguacaag ucccuggaga aggccaucaa ggaguucggg agccagaucc     2520

ucaaggagca ccccaccgac aaccaggagc ugcgcaacaa ccggcuguac cucuacuacc     2580

ugcagaacgg caaggacaug uacacgggcc aggaccugga cauccacaac cucuccaacu     2640

acgacaucga ccacaucgug ccccagagcu ucaucaccga caacuccauc gacaaccugg     2700

ugcugaccag cuccgccggg aaccgcgaga agggcgacga cgucccgccc cucgagaucg     2760

ugcggaagcg caagguguuc ugggagaagc uguaccaggg gaaccugaug agcaagcgca     2820

aguucgacua ccucaccaag gcggagcggg gcgggcugac cgaggccgac aaggcccgcu     2880

ucauccaccg gcagcugguc gagacgcgcc agaucaccaa gaacguggcc aacauccucc     2940

accagcgguu caacuacgag aaggacgacc acggcaacac caugaagcag guccgcaucg     3000

ugacccugaa guccgcgcug gugagccagu uccggaagca guuccagcuc uacaaggugc     3060

gcgacgucaa cgacuaccac cacgcccacg acgccuaccu gaacggcgug guggccaaca     3120

cgcugcucaa ggucuacccc cagcuggagc ccgaguucgu guacggggac uaccaccagu     3180

ucgacugguu caaggccaac aaggcgaccg ccaagaagca guucuacacc aacaucaugc     3240

uguucuucgc ccagaaggac cggaucaucg acgagaacgg cgagauccuc ugggacaaga     3300

aguaccugga caccgugaag aaggucaugu ccuaccgcca gaugaacauc gugaagaaga     3360

cggagaucca gaagggggag uucagcaagg ccaccaucaa gcccaagggc aacuccagca     3420

agcugauccc ccggaagacc aacugggacc cgaugaagua cgggggccuc gacagcccca     3480

acauggccua cgcggugguc aucgaguacg ccaagggcaa gaacaagcug guguucgaga     3540

agaagaucau ccgcgugacc aucauggagc ggaaggccuu cgagaaggac gagaaggccu     3600

uccuggagga gcagggguac cgccagccca agguccucgc caagcugccg aaguacacgc     3660

uguacgagug cgaggagggc cggcgccgga ugcucgcguc cgccaacgag gcccagaagg     3720

ggaaccagca gguccugccc aaccaccugg ugacccuccu gcaccacgcc gcgaacugcg     3780

aggugagcga cggcaagucc cuggacuaca ucgagagcaa ccgcgagaug uucgccgagc     3840

uccuggccca cguguccgag uucgccaagc gguacacccu ggccgaggcg aaccucaaca     3900

agaucaacca gcuguucgag cagaacaagg agggggacau caaggccauc gcccagagcu     3960

ucguggaccu gauggccuuc aacgccaugg gcgcgcccgc cuccuucaag uucuucgaga     4020

ccacgaucga gcgcaagcgg uacaacaacc ucaaggagcu gcugaacagc accaucaucu     4080

accaguccau caccggccuc uacgagagcc gcaagcgccu ggacgacaag cggcccgccg     4140

ccaccaagaa ggccgggcag gcgaagaaga agaaguaagg acuagugucc accugucccu     4200

ccugggcugc uggauugucu cguuuuccug ccaaauaaac aggaucagcg cuuuacagau     4260

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaagaau u                                                          4331


<210>  2530
<211>  4364
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2530
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa gaagcccuac agcaucggcc uggacaucgg gaccaacucc gugggcuggg      180

ccguggucac cgacgacuac aaggugcccg ccaagaagau gaaggugcuc gggaacacgg      240

acaagagcca caucgagaag aaccugcugg gcgcccuccu guucgacucc ggcaacaccg      300

ccgaggaccg gcgccugaag cggaccgcgc gccggcgcua cacccggcgc cggaaccgca      360

uccucuaccu gcaggagauc uucagcgagg agauggggaa ggucgacgac uccuucuucc      420

accggcugga ggacagcuuc cucgugacgg aggacaagcg cggcgagcgg caccccaucu      480

ucgggaaccu ggaggaggag gugaaguacc acgagaacuu cccgaccauc uaccaccucc      540

gccaguaccu ggccgacaac cccgagaagg ucgaccugcg gcugguguac cucgcccugg      600

cccacaucau caaguuccgc ggccacuucc ugaucgaggg gaaguucgac acccggaaca      660

acgacgugca gcgccucuuc caggaguucc uggcggucua cgacaacacc uucgagaacu      720

ccagccugca ggagcagaac guccaggugg aggagauccu cacggacaag aucuccaaga      780

gcgccaagaa ggaccgggug cugaagcugu uccccaacga gaaguccaac ggccgcuucg      840

ccgaguuccu caagcugauc gugggcaacc aggccgacuu caagaagcac uucgagcugg      900

aggagaaggc cccgcuccag uucagcaagg acaccuacga ggaggagcug gaggugcugc      960

ucgcgcagau cggggacaac uacgccgagc uguuccuguc cgccaagaag cucuacgaca     1020

gcauccugcu guccggcauc cucaccguca ccgacguggg gacgaaggcc ccgcugagcg     1080

ccuccaugau ccagcgguac aacgagcacc agauggaccu ggcgcagcuc aagcaguuca     1140

uccgccagaa gcugagcgac aaguacaacg agguguucuc cgacgucagc aaggacggcu     1200

acgccggcua caucgacggg aagaccaacc aggaggccuu cuacaaguac cugaagggcc     1260

uccugaacaa gaucgagggg agcggcuacu uccuggacaa gaucgagcgc gaggacuucc     1320

uccggaagca gcgcaccuuc gacaacgggu ccauccccca ccagauccac cuccaggaga     1380

ugcgggccau cauccgccgg caggccgagu ucuaccccuu ccuggcggac aaccaggacc     1440

gcaucgagaa gcugcugacc uuccggaucc ccuacuacgu gggcccgcug gcccgcggca     1500

agagcgacuu cgccuggcug ucccgcaaga gcgccgacaa gaucacgccc uggaacuucg     1560

acgagaucgu ggacaaggag uccagcgccg aggcguucau caaccggaug accaacuacg     1620

accucuaccu gcccaaccag aagguccugc ccaagcacuc ccuccuguac gagaaguuca     1680

ccguguacaa cgagcugacc aaggucaagu acaagacgga gcaggggaag accgccuucu     1740

ucgacgccaa caugaagcag gagaucuucg acggcguguu caagguguac cggaagguca     1800

ccaaggacaa gcucauggac uuccuggaga aggaguucga cgaguuccgc aucguggacc     1860

ugaccgggcu cgacaaggag aacaaggugu ucaacgccag cuacggcacg uaccacgacc     1920

ugugcaagau ccuggacaag gacuuccucg acaacuccaa gaacgagaag auccuggagg     1980

acaucguccu gacccucacc cuguucgagg accgggagau gauccgcaag cggcuggaga     2040

acuacagcga ccuccugacc aaggagcagg ucaagaagcu ggagcgccgg cacuacacgg     2100

gguggggccg ccucuccgcg gagcugaucc acggcauccg gaacaaggag agccgcaaga     2160

ccauccugga cuaccucauc gacgacggga acuccaaccg caacuucaug cagcugauca     2220

acgacgacgc ccugagcuuc aaggaggaga ucgccaaggc ccaggugauc ggcgagaccg     2280

acaaccucaa ccagguggug uccgacaucg ccgggagccc ggcgaucaag aagggcaucc     2340

ugcagucccu gaagaucgug gacgagcucg ucaagaucau ggggcaccag cccgagaaca     2400

ucguggugga gauggcccgg gagaaccagu ucaccaacca gggccgccgg aacagccagc     2460

agcgccugaa gggccugacg gacuccauca aggaguucgg gagccagauc cucaaggagc     2520

accccgucga gaacagccag cugcagaacg accggcuguu ccucuacuac cugcagaacg     2580

gccgcgacau guacaccggg gaggagcugg acaucgacua ccucucccag uacgacaucg     2640

accacaucau cccccaggcc uucaucaagg acaacagcau cgacaaccgg gugcugaccu     2700

ccagcaagga gaaccgcggc aaguccgacg acgugccgag caaggacguc gugcggaaga     2760

ugaaguccua cuggagcaag cugcucuccg ccaagcugau cacccagcgc aaguucgaca     2820

accugacgaa ggccgagcgg ggcgggcuca ccgacgacga caaggcgggc uucaucaagc     2880

gccagcuggu ggagacccgg cagaucacca agcacgucgc ccgcauccug gacgagcggu     2940

ucaacaccga gacggacgag aacaacaaga agauccgcca ggugaagauc gugacccuca     3000

agagcaaccu ggucuccaac uuccggaagg aguucgagcu guacaaggug cgcgagauca     3060

acgacuacca ccacgcccac gacgccuacc ucaacgccgu gaucgggaag gcgcugcugg     3120

gcgucuaccc ccagcucgag cccgaguucg uguacgggga cuacccccac uuccacggcc     3180

acaaggagaa caaggccacc gccaagaagu ucuucuacag caacaucaug aacuucuuca     3240

agaaggacga cgugcggacc gacaagaacg gcgagaucau cuggaagaag gacgagcaca     3300

ucuccaacau caagaagguc cugagcuacc cccaggugaa caucgugaag aaggucgagg     3360

agcagaccgg gggcuucucc aaggagagca uccugcccaa ggggaacucc gacaagcuca     3420

ucccccgcaa gacgaagaag uucuacuggg acaccaagaa guacggcggg uucgacagcc     3480

ccaucguggc cuacagcauc cuggugaucg cggacaucga gaagggcaag uccaagaagc     3540

ugaagaccgu caaggcccuc gugggcguga cgaucaugga gaagaugacc uucgagcggg     3600

acccggucgc cuuccuggag cgcaaggggu accgcaacgu gcaggaggag aacaucauca     3660

agcugcccaa guacagccuc uucaagcugg agaacggccg gaagcgccug cucgccuccg     3720

cccgggagcu gcagaagggg aacgagaucg ugcugcccaa ccaccucggc acccugcugu     3780

accacgcgaa gaacauccac aaggucgacg agccgaagca ccucgacuac guggacaagc     3840

acaaggacga guucaaggag cugcuggacg uggucagcaa cuucuccaag aaguacaccc     3900

ucgccgaggg gaaccuggag aagaucaagg agcuguacgc ccagaacaac ggcgaggacc     3960

ucaaggagcu ggccagcucc uucaucaacc ugcucacguu caccgccauc ggcgcgcccg     4020

ccaccuucaa guucuucgac aagaacaucg accgcaagcg guacaccagc acgaccgaga     4080

uccugaacgc cacccugauc caccagucca ucaccgggcu cuacgagacg cgcaucgacc     4140

ugaacaagcu gggcggggac aagcggcccg ccgccaccaa gaaggcgggc caggccaaga     4200

agaagaagua aggacuagug uccaccuguc ccuccugggc ugcuggauug ucucguuuuc     4260

cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4364


<210>  2531
<211>  3497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2531
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgca gaccaccaac cugagcuaca uccucggccu ggaccugggg aucgccuccg      180

ugggcugggc cguggucgag aucaacgaga acgaggaccc caucgggcuc aucgacgugg      240

gcgugcggau cuucgagcgc gccgaggucc ccaagacggg cgagagccug gcccuguccc      300

ggcgccucgc gcggagcacc cgccggcuga uccgccggcg cgcccaccgg cugcuccugg      360

ccaagcgcuu ccugaagcgg gaggggaucc ucuccaccau cgaccuggag aagggccugc      420

ccaaccaggc cugggagcuc cgcguggcgg ggcuggagcg gcgccugagc gccaucgagu      480

ggggcgccgu gcuccugcac cugaucaagc accgggggua ccucuccaag cgcaagaacg      540

agagccagac caacaacaag gagcugggcg cccugcucuc cggcgucgcc cagaaccacc      600

agcugcugca gagcgacgac uaccggacgc cggcggagcu cgcccugaag aaguucgcca      660

aggaggaggg gcacauccgc aaccagcggg gcgccuacac ccacaccuuc aaccgccugg      720

accuccuggc cgagcugaac cuccuguucg cgcagcagca ccaguucggg aacccccacu      780

gcaaggagca cauccagcag uacaugaccg agcugcucau guggcagaag cccgcccugu      840

ccggcgaggc cauccugaag augcucggca agugcacgca cgagaagaac gaguucaagg      900

ccgccaagca caccuacagc gcggagcgcu ucguguggcu gaccaagcug aacaaccucc      960

ggauccugga ggacggggcc gagcgcgccc ugaacgagga ggagcggcag cuccugauca     1020

accaccccua cgagaagucc aagcugaccu acgcccaggu gcgcaagcuc cugggccuga     1080

gcgagcaggc caucuucaag caccuccggu acuccaagga gaacgcggag agcgccacgu     1140

ucauggagcu caaggccugg cacgccaucc gcaaggcgcu ggagaaccag gggcugaagg     1200

acaccuggca ggaccucgcc aagaagccgg accugcugga cgagaucggc accgccuucu     1260

cccucuacaa gaccgacgag gacauccagc aguaccugac gaacaagguc cccaacagcg     1320

ugaucaacgc ccugcucgug ucccugaacu ucgacaaguu caucgagcug agccucaaga     1380

gccugcggaa gauccugccc cucauggagc aggggaagcg cuacgaccag gccugccggg     1440

agaucuacgg ccaccacuac ggcgaggcga accagaagac cucccagcug cugcccgcca     1500

ucccggccca ggagauccgc aaccccgucg ugcuccggac ccugagccag gcccgcaagg     1560

ugaucaacgc caucauccgg caguacgggu cccccgcgcg cguccacauc gagaccggcc     1620

gggagcuggg caagagcuuc aaggagcgcc gggagaucca gaagcagcag gaggacaacc     1680

gcacgaagcg ggaguccgcc gugcagaagu ucaaggagcu guucagcgac uucuccagcg     1740

agcccaaguc caaggacauc cugaaguucc gccuguacga gcagcagcac gggaagugcc     1800

ucuacagcgg gaaggagauc aacauccacc gccugaacga gaagggcuac guggagaucg     1860

accacgcccu gccguucucc cggaccuggg acgacagcuu caacaacaag guccucgugc     1920

uggccuccga gaaccagaac aagggcaacc agacccccua cgaguggcug caggggaaga     1980

ucaacagcga gcgcuggaag aacuucgugg cccucguccu gggcucccag ugcagcgcgg     2040

ccaagaagca gcggcugcuc acccagguga ucgacgacaa caaguucauc gaccgcaacc     2100

ugaacgacac gcgguacauc gcccgcuucc uguccaacua cauccaggag aaccuccugc     2160

uggucgggaa gaacaagaag aacguguuca cccccaacgg ccagaucacc gcccuccugc     2220

ggagccgcug ggggcugauc aaggcgcggg agaacaacaa ccgccaccac gcccucgacg     2280

ccaucguggu cgccugcgcc acccccucca ugcagcagaa gaucacgcgg uucauccgcu     2340

ucaaggaggu gcacccguac aagaucgaga accgguacga gaugguggac caggagagcg     2400

gcgagaucau cagcccccac uuccccgagc ccugggcgua cuuccgccag gaggucaaca     2460

uccggguguu cgacaaccac ccggacaccg ugcugaagga gaugcugccc gaccgccccc     2520

aggccaacca ccaguucguc cagccccucu ucgugucccg ggccccgacc cgcaagauga     2580

gcggccaggg gcacauggag accaucaagu ccgccaagcg gcuggccgag ggcaucagcg     2640

ugcugcgcau cccccucacg cagcugaagc ccaaccugcu cgagaacaug gucaacaagg     2700

agcgggagcc ggcgcuguac gccgggcuga aggcccgccu cgccgaguuc aaccaggacc     2760

ccgccaaggc guucgccacc cccuucuaca agcagggcgg ccagcagguc aaggccaucc     2820

gcguggagca gguccagaag uccggggugc uggugcggga gaacaacggc guggccgaca     2880

acgcgagcau cgugcgcacc gacguguuca ucaagaacaa caaguucuuc cuggucccca     2940

ucuacaccug gcaggucgcc aaggggaucc ucccgaacaa ggccaucgug gcccacaaga     3000

acgaggacga gugggaggag auggacgagg gcgccaaguu caaguucucc cuguucccca     3060

acgaccuggu ggagcucaag acgaagaagg aguacuucuu cggguacuac aucggccugg     3120

accgggcgac cggcaacauc agccugaagg agcacgacgg ggagaucucc aagggcaagg     3180

acggggugua ccgcgugggc gucaagcucg cccugagcuu cgagaaguac cagguggacg     3240

agcuggggaa gaaccggcag aucugccgcc cccagcagcg gcagcccgug cgcaagcggc     3300

cggccgccac caagaaggcc ggccaggcga agaagaagaa guaaggacua guguccaccu     3360

gucccuccug ggcugcugga uugucucguu uuccugccaa auaaacagga ucagcgcuuu     3420

acagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aagaauu                                                    3497


<210>  2532
<211>  3575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2532
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc cgccuucaag cccaacccca ucaacuacau ccugggccuc gacaucggga      180

ucgccagcgu gggcugggcc augguggaga ucgacgagga cgagaacccc aucugccuga      240

ucgaccuggg gguccgggug uucgagcgcg cggaggugcc gaagaccggc gacucccucg      300

ccauggcccg gcgccuggcc cggagcgucc gccggcugac ccgccggcgc gcgcaccggc      360

uccugcgcgc ccggcgccug cucaagcggg agggcgugcu gcaggccgcc gacuucgacg      420

agaacgggcu gaucaagucc cuccccaaca cgcccuggca gcugcgcgcc gcggcccugg      480

accggaagcu cacgccccug gaguggagcg ccgugcugcu ccaccugauc aagcaccgcg      540

gcuaccuguc ccagcggaag aacgaggggg agaccgccga caaggagcuc ggcgcccugc      600

ugaagggggu cgcggacaac gcccacgccc uccagaccgg cgacuuccgc accccggccg      660

agcuggcccu gaacaaguuc gagaaggaga gcggccacau ccggaaccag cgcggggacu      720

acucccacac cuucagccgc aaggaccucc aggcggagcu gauccuccuc uucgagaagc      780

agaaggaguu cggcaacccc cacguguccg ggggccugaa ggagggcauc gagacccugc      840

ucaugaccca gcggcccgcc cugagcgggg acgccgugca gaagaugcug ggccacugca      900

cguucgagcc cgccgagccg aaggccgcga agaacaccua caccgccgag cgcuucaucu      960

ggcucaccaa gcugaacaac cugcggaucc ucgagcaggg guccgagcgc ccccugacgg     1020

acaccgagcg ggccacccug auggacgagc ccuaccgcaa gagcaagcuc accuacgccc     1080

aggcgcggaa gcugcugggc cucgaggaca cggccuucuu caaggggcug cgcuacggca     1140

aggacaacgc cgaggccucc acccugaugg agaugaaggc cuaccacgcg aucagccggg     1200

cccucgagaa ggagggccug aaggacaaga aguccccgcu gaaccugagc cccgagcucc     1260

aggacgagau cgggaccgcc uucucccugu ucaagaccga cgaggacauc acgggccgcc     1320

ugaaggaccg gauccagccc gagauccucg aggcccugcu gaagcacauc agcuucgaca     1380

aguucgucca gaucucccuc aaggcccugc gccggaucgu gccccugaug gagcagggga     1440

agcgcuacga cgaggcgugc gccgagaucu acggcgacca cuacgggaag aagaacaccg     1500

aggagaagau cuaccugccc ccgauccccg ccgacgagau ccggaacccc gugguccugc     1560

gcgcccugag ccaggcccgg aaggugauca acggcguggu ccgccgguac ggcagccccg     1620

cgcgcaucca caucgagacc gcccgcgagg uggggaaguc cuucaaggac cggaaggaga     1680

ucgagaagcg ccaggaggag aaccggaagg accgcgagaa ggccgccgcg aaguuccggg     1740

aguacuuccc gaacuucgug ggcgagccca agagcaagga cauccucaag cugcgccugu     1800

acgagcagca gcacgggaag ugccucuacu ccggcaagga gaucaaccug gggcggcuga     1860

acgagaaggg cuacgucgag aucgaccacg cccuccccuu cagccgcacc ugggacgacu     1920

ccuucaacaa caaggugcug gugcugggca gcgagaacca gaacaagggg aaccagacgc     1980

ccuacgagua cuucaacggc aaggacaacu cccgggagug gcaggaguuc aaggcccgcg     2040

ucgagaccag ccgguucccg cgcuccaaga agcagcggau ccuccugcag aaguucgacg     2100

aggacggguu caaggagcgc aaccugaacg acacccggua cgugaaccgc uuccucugcc     2160

aguucgucgc cgaccggaug cgccugaccg gcaagggcaa gaagcgggug uucgccagca     2220

acgggcagau cacgaaccug cuccgcggcu ucugggggcu gcggaaggug cgcgcggaga     2280

acgaccgcca ccacgcccug gacgccgucg ugguggccug cuccaccguc gccaugcagc     2340

agaagaucac ccgguucgug cgcuacaagg agaugaacgc guucgacggc aagaccaucg     2400

acaaggagac gggggaggug cuccaccaga agacccacuu cccccagccc ugggaguucu     2460

ucgcccagga ggucaugauc cggguguucg gcaagccgga cggcaagccc gaguucgagg     2520

aggccgacac ccccgagaag cugcgcaccc ugcucgccga gaagcugagc ucccggcccg     2580

aggccgugca cgaguacguc acgccgcugu ucgugagccg cgcgcccaac cggaagaugu     2640

ccgggcaggg ccacauggag accgucaaga gcgccaagcg ccucgacgag gggguguccg     2700

ugcugcgggu gccccugacc cagcucaagc ugaaggaccu ggagaagaug gucaaccgcg     2760

agcgggagcc caagcucuac gaggcccuga aggcccgccu ggaggcgcac aaggacgacc     2820

cggccaaggc cuucgccgag cccuucuaca aguacgacaa ggccggcaac cggacccagc     2880

aggucaaggc ggugcgcgug gagcaggucc agaagaccgg ggugugggug cggaaccaca     2940

acggcaucgc cgacaacgcc acgauggugc gcguggacgu cuucgagaag ggcgacaagu     3000

acuaccucgu gcccaucuac agcuggcagg ucgccaaggg gauccugccc gaccgggccg     3060

uggugcaggg caaggacgag gaggacuggc agcugaucga cgacuccuuc aacuucaagu     3120

ucagccucca cccgaacgac cugguggagg ucaucaccaa gaaggcgcgc auguucgggu     3180

acuucgccag cugccaccgg ggcaccggga acaucaacau ccgcauccac gaccuggacc     3240

acaagaucgg caagaacggc auccucgagg ggaucggcgu gaagaccgcc cuguccuucc     3300

agaaguacca gaucgacgag cuggggaagg agauccgccc cugccggcuc aagaagcgcc     3360

cgcccgugcg gaagcgcccc gccgccacga agaaggcggg ccaggccaag aagaagaagu     3420

aaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu ccugccaaau     3480

aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                3575


<210>  2533
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2533
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgaa guaccacgug ggcaucgacg uggggaccuu cagcgucggc cuggccgcca      180

ucgaggugga cgacgccggg augcccauca agacccuguc ccuggugagc cacauccacg      240

acuccggccu ggaccccgac gagaucaaga gcgccgucac gcggcucgcg uccagcggca      300

ucgcccgccg gacccgccgg cuguaccgcc ggaagcgccg gcgccugcag cagcucgaca      360

aguucaucca gcggcagggg uggcccguga ucgagcugga ggacuacucc gacccgcugu      420

accccuggaa ggugcgcgcc gagcucgccg cgagcuacau cgccgacgag aaggagcggg      480

gcgagaagcu guccgucgcc cugcgccaca ucgcccggca ccgcgggugg cggaaccccu      540

acgccaaggu gagcucccuc uaccugcccg acggcccgag cgacgcguuc aaggccaucc      600

gcgaggagau caagcgggcc uccgggcagc ccgugcccga gaccgccacc gucggccaga      660

uggugacgcu gugcgagcuc ggcacccuga agcugcgcgg ggagggcggg gugcucagcg      720

cccggcugca gcaguccgac uacgcgcgcg agauccagga gaucugccgc augcaggaga      780

ucggccagga gcuguaccgg aagaucaucg acgucguguu cgccgccgag agccccaagg      840

gcuccgccag cucccgcgug gggaaggacc cgcuccagcc cggcaagaac cgggcccuga      900

aggcgagcga cgccuuccag cgcuaccgga ucgccgcccu gaucgggaac cuccgcgucc      960

ggguggacgg cgagaagcgc auccugagcg uggaggagaa gaaccugguc uucgaccacc     1020

ucgugaaccu gacccccaag aaggagcccg agugggugac caucgccgag auccugggga     1080

ucgaccgggg ccagcucauc ggcacggcga ccaugaccga cgacggggag cgcgccggcg     1140

cccggccgcc cacccacgac acgaaccgcu ccaucgucaa cagccggauc gcgccccugg     1200

uggacuggug gaagaccgcc uccgcccugg agcagcacgc cauggucaag gcccucagca     1260

acgccgaggu ggacgacuuc gacucccccg agggggcgaa ggugcaggcc uucuucgccg     1320

accuggacga cgacguccac gccaagcugg acagccucca ccucccggug ggccgcgccg     1380

cguacuccga ggacacccug gugcggcuca cccgccggau gcugagcgac ggggucgacc     1440

uguacacggc ccgccuccag gaguucggca ucgagcccuc cuggaccccg cccacgcccc     1500

ggaucggcga gcccgugggg aaccccgccg uggaccgcgu ccugaagacc gugagccgcu     1560

ggcuggaguc cgccaccaag accuggggcg cccccgagcg ggugaucauc gagcacgucc     1620

gcgagggguu cgugaccgag aagcgggcgc gcgagaugga cggcgacaug cggcgccggg     1680

ccgcccgcaa cgccaagcug uuccaggaga ugcaggagaa gcugaacgug caggggaagc     1740

cgagccgggc ggaccugugg cgcuaccagu ccguccagcg gcagaacugc cagugcgccu     1800

acugcggcag ccccaucacc uucuccaaca gcgagaugga ccacaucgug ccccgcgccg     1860

gccagggguc cacgaacacc cgggagaacc ucguggccgu cugccaccgc ugcaaccaga     1920

gcaagggcaa caccccguuc gccaucuggg cgaagaacac cuccaucgag ggggugagcg     1980

ugaaggaggc cgucgagcgg acgcgccacu gggugaccga caccggcaug cggagcaccg     2040

acuucaagaa guucacgaag gccguggucg agcgcuucca gcgggccacc auggacgagg     2100

agaucgacgc ccgcuccaug gagagcgugg cguggauggc caacgagcug cggucccgcg     2160

uggcccagca cuucgccagc cacggcacca ccguccgcgu guaccggggg ucccugacgg     2220

ccgaggcgcg ccgggccagc ggcaucuccg ggaagcugaa guucuucgac ggcgugggca     2280

agagccgccu cgaccggcgc caccacgcca ucgacgccgc ggucaucgcc uucaccuccg     2340

acuacguggc cgagacccug gccgugcgga gcaaccugaa gcagucccag gcccaccgcc     2400

aggaggcgcc ccaguggcgg gaguucaccg ggaaggacgc cgagcaccgc gccgccuggc     2460

gggucuggug ccagaagaug gagaagcuca gcgcccugcu gaccgaggac cuccgcgacg     2520

accggguggu ggucaugucc aacgugcgcc ugcggcuggg caacgggagc gcgcacaagg     2580

agacgaucgg caagcucucc aaggugaagc ugagcuccca gcugagcguc agcgacaucg     2640

acaaggccuc cagcgaggcc cucuggugcg cccugacccg cgagcccggg uucgacccca     2700

aggagggccu gccggccaac cccgagcggc acauccgcgu gaacgggacc cacguguacg     2760

cgggcgacaa caucggccuc uuccccgucu ccgccgggag caucgcccug cggggcgggu     2820

acgccgagcu gggcuccagc uuccaccacg cccgcgugua caagaucacc uccgggaaga     2880

agcccgcguu cgccaugcuc cgcguguaca cgaucgaccu gcugccguac cggaaccagg     2940

accucuucag cgucgagcug aagccccaga ccauguccau gcgccaggcc gagaagaagc     3000

ugcgggacgc ccucgcgacc ggcaacgccg aguaccuggg cuggcuggug guggacgacg     3060

agcucgucgu ggacaccagc aagaucgcca cggaccaggu gaaggccguc gaggccgagc     3120

uggggaccau ccgccggugg cgcguggacg gcuucuucuc ccccagcaag cugcggcucc     3180

gcccccugca gauguccaag gaggggauca agaaggagag cgcgccggag cuguccaaga     3240

ucaucgaccg gcccggcugg cuccccgccg ucaacaagcu guucagcgac gggaacguga     3300

ccgugguccg ccgggacucc cugggccgcg ugcggcucga gagcaccgcc caccuccccg     3360

ugacguggaa gguccagaag cgcccggccg ccaccaagaa ggcgggccag gccaagaaga     3420

agaaguaagg acuagugucc accugucccu ccugggcugc uggauugucu cguuuuccug     3480

ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  2534
<211>  3281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2534
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcggc ccggauccug gccuucgaca ucggcaucag cuccaucggg ugggccuuca      180

gcgagaacga cgagcucaag gacugcggcg ugcgcaucuu caccaaggug gagaacccca      240

agaccgggga gucccuggcc cugccccggc gccucgcgcg gagcgcccgc aagcggcugg      300

cccgccggaa ggcccgccug aaccaccuca agcaccugau cgcgaacgag uucaagcuga      360

acuacgagga cuaccagucc uucgacgaga gccucgccaa ggccuacaag ggcucccuga      420

ucagccccua cgagcugcgg uuccgcgccc ucaacgagcu gcuguccaag caggacuucg      480

cccgggucau ccuccacauc gcgaagcgcc ggggcuacga cgacaucaag aacagcgacg      540

acaaggagaa gggggccauc cugaaggcca ucaagcagaa cgaggagaag cuggccaacu      600

accaguccgu gggcgaguac cucuacaagg aguacuucca gaaguucaag gagaacagca      660

aggaguucac gaacgugcgc aacaagaagg aguccuacga gcggugcauc gcccagagcu      720

uccugaagga cgagcugaag cucaucuuca agaagcagcg cgaguucggg uucuccuuca      780

gcaagaaguu cgaggaggag guccuguccg uggcguucua caagcgggcc cugaaggacu      840

ucagccaccu cgugggcaac ugcuccuucu ucaccgacga gaagcgcgcc ccgaagaaca      900

gcccccuggc cuucauguuc gucgcccuga cccggaucau caaccuccug aacaaccuga      960

agaacaccga ggggauccuc uacacgaagg acgaccugaa cgcgcugcuc aacgaggugc     1020

ugaagaacgg cacccugacc uacaagcaga ccaagaagcu ccugggccug agcgacgacu     1080

acgaguucaa gggggagaag ggcacguacu ucaucgaguu caagaaguac aaggaguuca     1140

ucaaggcccu cggggagcac aaccuguccc aggacgaccu gaacgagauc gccaaggaca     1200

ucacccucau caaggacgag aucaagcuga agaaggcccu ggccaaguac gaccucaacc     1260

agaaccagau cgacagccug uccaagcugg aguucaagga ccaccucaac aucagcuuca     1320

aggcgcugaa gcuggugacg ccccugaugc ucgagggcaa gaaguacgac gaggccugca     1380

acgagcugaa ccugaagguc gccaucaacg aggacaagaa ggacuuccuc cccgccuuca     1440

acgagaccua cuacaaggac gaggugacca acccgguggu ccugcgcgcg aucaaggagu     1500

accgcaaggu gcugaacgcc cuccugaaga aguacggcaa ggugcacaag aucaacaucg     1560

agcuggcccg ggaggucggg aagaaccacu cccagcgcgc caagaucgag aaggagcaga     1620

acgagaacua caaggccaag aaggacgcgg agcucgagug cgagaagcug ggccugaaga     1680

ucaacagcaa gaacauccuc aagcugcggc uguucaagga gcagaaggag uucugcgccu     1740

acuccgggga gaagaucaag aucagcgacc uccaggacga gaagaugcug gagaucgacc     1800

acaucuaccc cuacucccgc agcuucgacg acuccuacau gaacaaggug cugguguuca     1860

ccaagcagaa ccaggagaag cucaaccaga cccccuucga ggccuucggc aacgacagcg     1920

ccaaguggca gaagaucgag guccuggcca agaaccugcc caccaagaag cagaagcgga     1980

uccucgacaa gaacuacaag gacaaggagc agaagaacuu caaggaccgc aaccugaacg     2040

acacgcggua caucgcgcgc cuggugcuca acuacaccaa ggacuaccug gacuuccugc     2100

cgcucuccga cgacgagaac accaagcuga acgacaccca gaaggggagc aaggugcacg     2160

ucgaggccaa guccggcaug cugacgagcg cccuccggca caccuggggc uucuccgcca     2220

aggaccgcaa caaccaccuc caccacgcca ucgacgcggu gaucaucgcc uacgccaaca     2280

acagcaucgu caaggccuuc uccgacuuca agaaggagca ggagagcaac agcgcggagc     2340

uguacgccaa gaagaucucc gagcuggacu acaagaacaa gcggaaguuc uucgagcccu     2400

ucagcggguu ccgccagaag gugcuggaca agaucgacga gaucuucgug uccaagcccg     2460

agcggaagaa gcccagcggc gcccugcacg aggagaccuu ccgcaaggag gaggaguucu     2520

accaguccua cgggggcaag gagggggucc ucaaggcccu ggagcugggc aagauccgga     2580

aggugaacgg caagaucgug aagaacgggg acauguuccg cgucgacauc uucaagcaca     2640

agaagaccaa caaguucuac gccgugccga ucuacacgau ggacuucgcg cucaaggugc     2700

ugcccaacaa ggccgucgcc cggagcaaga agggcgagau caaggacugg auccugaugg     2760

acgagaacua cgaguucugc uucucccucu acaaggacag ccugauccug auccagacca     2820

aggacaugca ggagcccgag uucguguacu acaacgccuu caccuccagc accguguccc     2880

ucaucgucag caagcacgac aacaaguucg agacgcuguc caagaaccag aagauccugu     2940

ucaagaacgc caacgagaag gaggugaucg ccaagagcau cgggauccag aaccucaagg     3000

uguucgagaa guacaucguc uccgcgcugg gcgaggugac caaggccgag uuccgccagc     3060

gggaggacuu caagaagaag cgccccgccg ccaccaagaa ggcggggcag gccaagaaga     3120

agaaguaagg acuagugucc accugucccu ccugggcugc uggauugucu cguuuuccug     3180

ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3281


<210>  2535
<211>  3692
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  2535
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgag cgaccuggug cucggccugg acaucgggau cggcuccgug ggggucggca      180

uccugaacaa ggugaccggc gagaucaucc acaagaacag ccggaucuuc cccgccgccc      240

aggccgagaa caaccucgug cgccggacca accgccaggg gcggcgccug gcccggcgca      300

agaagcaccg gcgcguccgg cugaaccgcc ucuucgagga guccggccug aucacggacu      360

ucaccaagau cagcaucaac cugaaccccu accagcuccg ggugaagggg cugaccgacg      420

agcuguccaa cgaggagcug uucaucgcgc ugaagaacau ggugaagcac cgcggcauca      480

gcuaccugga cgacgccucc gacgacggga acagcuccgu cggcgacuac gcccagaucg      540

ugaaggagaa cagcaagcag cucgagacca agacgcccgg ccagauccag cuggagcggu      600

accagaccua cgggcagcug cgcggcgacu ucaccgugga gaaggacggg aagaagcacc      660

ggcucaucaa cgucuucccg accuccgccu accgcagcga ggcgcugcgg auccugcaga      720

cgcagcagga guucaacccc cagaucaccg acgaguucau caaccgcuac cucgagaucc      780

ugaccggcaa gcggaaguac uaccacggcc ccgggaacga gaagucccgc accgacuacg      840

gccgcuaccg gacgagcggg gagacccugg acaacaucuu cggcauccuc aucgggaagu      900

gcaccuucua ccccgacgag uuccgcgccg ccaaggccuc cuacaccgcc caggaguuca      960

accugcugaa cgaccucaac aaccugacgg ugccgaccga gaccaagaag cugagcaagg     1020

agcagaagaa ccagaucauc aacuacguga agaacgagaa ggcgaugggc cccgccaagc     1080

uguucaagua caucgccaag cuccuguccu gcgacgucgc cgacaucaag ggcuaccgga     1140

ucgacaagag cgggaaggcc gagauccaca ccuucgaggc guaccgcaag augaagacgc     1200

ucgagacccu ggacaucgag cagauggacc gggagacccu ggacaagcuc gccuacgugc     1260

ugacccugaa cacggagcgc gagggcaucc aggaggcccu cgagcacgag uucgccgacg     1320

gguccuucag ccagaagcag gucgacgagc uggugcaguu ccggaaggcc aacagcucca     1380

ucuucggcaa gggguggcac aacuucagcg ugaagcugau gauggagcuc auccccgagc     1440

uguacgagac cuccgaggag cagaugacca uccugacccg ccucggcaag cagaagacga     1500

ccagcuccag caacaagacc aaguacaucg acgagaagcu gcugaccgag gagaucuaca     1560

accccguggu cgcgaagucc gugcggcagg ccaucaagau cgugaacgcc gccaucaagg     1620

aguacggcga cuucgacaac aucgucaucg agauggcccg cgagacgaac gaggacgacg     1680

agaagaaggc gauccagaag auccagaagg ccaacaagga cgagaaggac gccgccaugc     1740

ucaaggcggc caaccaguac aacgggaagg ccgagcugcc gcacagcgug uuccacggcc     1800

acaagcagcu ggccaccaag auccggcugu ggcaccagca gggggagcgc ugccucuaca     1860

ccggcaagac caucuccauc cacgaccuga ucaacaacag caaccaguuc gaggucgacc     1920

acauccugcc ccucuccauc acguucgacg acagccuggc caacaaggug cugguguacg     1980

cgaccgccaa ccaggagaag gggcagcgga cccccuacca ggcccucgac uccauggacg     2040

acgccuggag cuuccgcgag cugaaggccu ucguccggga guccaagacc cugagcaaca     2100

agaagaagga guaccuccug accgaggagg acaucuccaa guucgacgug cgcaagaagu     2160

ucaucgagcg gaaccuggug gacacgcgcu acgcgagccg ggucgugcuc aacgcccugc     2220

aggagcacuu ccgcgcccac aagaucgaca ccaagguguc cgucgugcgc ggccaguuca     2280

ccagccagcu gcggcgccac uggggcaucg agaagacccg ggacacguac caccaccacg     2340

ccguggacgc ccucaucauc gcggccagcu cccagcugaa ccuguggaag aagcagaaga     2400

acacccucgu cagcuacucc gaggaccagc ugcuggacau cgagaccggg gagcucauca     2460

gcgacgacga guacaaggag uccguguuca aggcccccua ccagcacuuc guggacaccc     2520

ucaagagcaa ggaguucgag gacuccaucc uguucagcua ccaggucgac uccaaguuca     2580

accgcaagau cagcgacgcc acgaucuacg cgacccggca ggccaaggug ggcaaggaca     2640

aggccgacga gaccuacgug cucgggaaga ucaaggacau cuacacccag gacggcuacg     2700

acgccuucau gaagaucuac aagaaggaca aguccaaguu ccugauguac cgccacgacc     2760

cgcagacguu cgagaagguc aucgagccca uccuggagaa cuaccccaac aagcagauca     2820

acgagaaggg caaggaggug cccugcaacc cguuccucaa guacaaggag gagcacgggu     2880

acauccggaa guacagcaag aagggcaacg ggcccgagau caagucccug aaguacuacg     2940

acagcaagcu gggcaaccac aucgacauca cccccaagga cuccaacaac aagguggucc     3000

uccagagcgu gucccccugg cgcgccgacg uguacuucaa caagaccacc gggaaguacg     3060

agauccuggg ccugaaguac gcggaccucc aguucgagaa gggcacgggg accuacaaga     3120

ucagccagga gaaguacaac gacaucaaga agaaggaggg cgucgacagc gacuccgagu     3180

ucaaguucac ccuguacaag aacgaccugc uccuggugaa ggacaccgag acgaaggagc     3240

agcagcuguu ccgguuccuc agccgcacca ugccgaagca gaagcacuac guggagcuga     3300

agcccuacga caagcagaag uucgagggcg gggaggcccu gaucaagguc cucgggaacg     3360

uggccaacuc cggccagugc aagaagggcc ugggcaagag caacaucucc aucuacaagg     3420

ugcggaccga cguccugggg aaccagcaca ucaucaagaa cgagggggac aagcccaagc     3480

ucgacuucaa gcgccccgcc gccaccaaga aggcgggcca ggccaagaag aagaaguaag     3540

gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu gccaaauaaa     3600

caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3692


<210>  2536
<211>  4250
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2536
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgac ccaguucgag ggcuucacca accuguacca ggugagcaag acgcuccggu      180

ucgagcugau cccccagggg aagacccuga agcacaucca ggagcagggc uucaucgagg      240

aggacaaggc ccgcaacgac cacuacaagg agcucaagcc caucaucgac cggaucuaca      300

agaccuacgc cgaccagugc cugcagcugg ugcagcucga cugggagaac cuguccgccg      360

ccaucgacag cuaccgcaag gagaagaccg aggagacgcg gaacgcgcug aucgaggagc      420

aggccaccua ccgcaacgcc auccacgacu acuucaucgg gcggaccgac aaccucaccg      480

acgccaucaa caagcgccac gcggagaucu acaagggccu guucaaggcc gagcuguuca      540

acggcaaggu ccucaagcag cuggggacgg ugaccaccac cgagcacgag aacgcccugc      600

uccgguccuu cgacaaguuc acgaccuacu ucagcggcuu cuacgagaac cgcaagaacg      660

uguucuccgc cgaggacauc agcaccgcca ucccccaccg gaucguccag gacaacuucc      720

cgaaguucaa ggagaacugc cacaucuuca cccgccugau cacggcggug cccucccugc      780

gggagcacuu cgagaacgug aagaaggcca ucgggaucuu cgucagcacc uccaucgagg      840

agguguucag cuuccccuuc uacaaccagc uccugaccca gacccagauc gaccuguaca      900

accagcuccu gggcgggauc ucccgcgagg ccggcacgga gaagaucaag ggccugaacg      960

aggugcucaa ccuggccauc cagaagaacg acgagaccgc ccacaucauc gcgagccugc     1020

cccaccgguu caucccccuc uucaagcaga uccuguccga ccgcaacacc cugagcuuca     1080

uccucgagga guucaagucc gacgaggagg ucauccagag cuucugcaag uacaagaccc     1140

ugcugcggaa cgagaacgug cucgagacgg ccgaggcccu guucaacgag cugaacucca     1200

ucgaccucac ccacaucuuc aucagccaca agaagcugga gaccaucucc agcgcccugu     1260

gcgaccacug ggacacccuc cgcaacgccc uguacgagcg gcgcaucagc gagcugacgg     1320

ggaagaucac caaguccgcg aaggagaagg ugcagcggag ccucaagcac gaggacauca     1380

accugcagga gaucaucucc gccgccggca aggagcugag cgaggccuuc aagcagaaga     1440

ccuccgagau ccucagccac gcccacgcgg cccuggacca gccgcugccc accacgcuca     1500

agaagcagga ggagaaggag auccugaagu cccagcugga cagccugcuc gggcuguacc     1560

accuccucga cugguucgcc gucgacgagu ccaacgaggu ggaccccgag uucagcgccc     1620

gccugaccgg caucaagcug gagauggagc cgucccucag cuucuacaac aaggcgcgca     1680

acuacgccac caagaagccc uacuccgugg agaaguucaa gcugaacuuc cagaugccca     1740

cccuggccag cggcugggac gucaacaagg agaagaacaa cggggccauc cucuucguga     1800

agaacggccu guacuaccug gggaucaugc ccaagcagaa gggccgguac aaggcccucu     1860

ccuucgagcc gacggagaag accagcgagg gguucgacaa gauguacuac gacuacuucc     1920

ccgacgcggc caagaugauc cccaagugcu ccacccagcu gaaggccgug accgcccacu     1980

uccagaccca cacgaccccc auccugcuca gcaacaacuu caucgagccg cuggagauca     2040

ccaaggagau cuacgaccug aacaaccccg agaaggagcc caagaaguuc cagaccgccu     2100

acgcgaagaa gacgggcgac cagaagggcu accgcgaggc ccucugcaag uggaucgacu     2160

ucacccggga cuuccugucc aaguacacca agaccacgag caucgaccug agcucccucc     2220

gccccagcuc ccaguacaag gaccuggggg aguacuacgc cgagcugaac ccgcuccugu     2280

accacaucag cuuccagcgg aucgccgaga aggagaucau ggacgccguc gagaccggca     2340

agcuguaccu cuuccagauc uacaacaagg acuucgcgaa ggggcaccac ggcaagccca     2400

accugcacac ccuguacugg accggccucu ucucccccga gaaccuggcc aagacgagca     2460

ucaagcugaa cgggcaggcc gagcuguucu accgccccaa gucccggaug aagcgcaugg     2520

cccaccggcu gggcgagaag augcugaaca agaagcucaa ggaccagaag accccgaucc     2580

ccgacacccu guaccaggag cuguacgacu acgugaacca ccgccucagc cacgaccugu     2640

ccgacgaggc gcgggcccug cuccccaacg ugaucaccaa ggaggucagc cacgagauca     2700

ucaaggaccg ccgguucacg uccgacaagu ucuucuucca cgugccgauc acccugaacu     2760

accaggccgc caacagcccc uccaaguuca accagcgcgu caacgccuac cugaaggagc     2820

accccgagac ccccaucauc gggaucgacc ggggcgagcg caaccucauc uacaucaccg     2880

ugaucgacag cacggggaag auccuggagc agcggucccu gaacaccauc cagcaguucg     2940

acuaccagaa gaagcucgac aaccgcgaga aggagcgggu ggcggcccgc caggccugga     3000

gcgucguggg caccaucaag gaccugaagc agggguaccu gucccaggug auccacgaga     3060

ucgucgaccu caugauccac uaccaggccg uggugguccu ggagaaccug aacuucggcu     3120

ucaagagcaa gcgcaccggc aucgccgaga aggcggugua ccagcaguuc gagaagaugc     3180

ucaucgacaa gcugaacugc cuggugcuca aggacuaccc ggccgagaag gucgggggcg     3240

ugcugaaccc cuaccagcug acggaccagu ucaccagcuu cgccaagaug gggacccagu     3300

ccggcuuccu cuucuacgug cccgcccccu acaccagcaa gaucgacccg cugacgggcu     3360

ucgucgaccc cuucgugugg aagaccauca agaaccacga gucccggaag cacuuccugg     3420

agggguucga cuuccuccac uacgacguga agaccggcga cuucauccug cacuucaaga     3480

ugaaccgcaa ccugagcuuc cagcgggggc uccccggcuu caugcccgcc ugggacaucg     3540

ucuucgagaa gaacgagacc caguucgacg cgaaggggac gccguucauc gccggcaagc     3600

gcaucgugcc cgugaucgag aaccaccggu ucaccggccg cuaccgggac cuguaccccg     3660

ccaacgagcu gaucgcccuc cuggaggaga aggggaucgu cuuccgcgac ggcuccaaca     3720

uccugcccaa gcuccuggag aacgacgaca gccacgcgau cgacaccaug guggcccuga     3780

uccgguccgu gcuccagaug cgcaacagca acgccgccac cggggaggac uacaucaacu     3840

ccccgguccg ggaccugaac ggcgugugcu ucgacagccg cuuccagaac cccgaguggc     3900

ccauggacgc cgacgcgaac ggggccuacc acaucgcccu gaagggccag cuccugcuga     3960

accaccucaa ggaguccaag gaccugaagc ugcagaacgg caucagcaac caggacuggc     4020

ucgccuacau ccaggagcug cggaacaagc gccccgccgc gacgaagaag gccgggcagg     4080

ccaagaagaa gaaguaagga cuagugucca ccugucccuc cugggcugcu ggauugucuc     4140

guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                4250


<210>  2537
<211>  4229
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  2537
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga agaagcgcaa ggugggcauc cacggggucc      120

cggccgcgag caucuaccag gaguucguga acaaguacuc ccugagcaag acccuccggu      180

ucgagcugau cccccagggc aagacccugg agaacaucaa ggcccgcggg cucauccugg      240

acgacgagaa gcgggccaag gacuacaaga aggccaagca gaucaucgac aaguaccacc      300

aguucuucau cgaggagauc cuguccagcg ugugcaucuc cgaggaccuc cugcagaacu      360

acagcgacgu cuacuucaag cugaagaagu ccgacgacga caaccuccag aaggacuuca      420

agagcgccaa ggacacgauc aagaagcaga ucuccgagua caucaaggac agcgagaagu      480

ucaagaaccu guucaaccag aaccugaucg acgcgaagaa gggccaggag uccgaccuca      540

uccuguggcu gaagcagagc aaggacaacg ggaucgagcu guucaaggcc aacuccgaca      600

ucaccgacau cgacgaggcc cucgagauca ucaagagcuu caagggcugg accaccuacu      660

ucaagggcuu ccacgagaac cgcaagaacg uguacuccag caacgacauc cccacgucca      720

ucaucuaccg gaucguggac gacaaccugc ccaaguuccu cgagaacaag gccaaguacg      780

agagccugaa ggacaaggcg ccggaggcca ucaacuacga gcagaucaag aaggaccugg      840

ccgaggagcu caccuucgac aucgacuaca agaccuccga ggucaaccag cgcguguuca      900

gccuggacga gguguucgag aucgccaacu ucaacaacua ccugaaccag agcgggauca      960

ccaaguucaa cacgaucauc ggcgggaagu ucgucaacgg cgagaacacc aagcggaagg     1020

ggaucaacga guacaucaac cucuacuccc agcagaucaa cgacaagacc cugaagaagu     1080

acaagaugag cgugcuguuc aagcagaucc ucuccgacac cgagagcaag uccuucguga     1140

ucgacaagcu ggaggacgac agcgacgucg ugacgaccau gcaguccuuc uacgagcaga     1200

ucgccgcguu caagaccgug gaggagaagu ccaucaagga gacccugucc cuccuguucg     1260

acgaccugaa ggcccagaag cucgaccuga gcaagaucua cuucaagaac gacaaguccc     1320

ugacggaccu cagccagcag gucuucgacg acuacuccgu gaucggcacc gccgugcugg     1380

aguacaucac ccagcagauc gcccccaaga accuggacaa ccccagcaag aaggagcagg     1440

agcucaucgc caagaagacc gagaaggcga aguaccuguc ccuggagacg aucaagcucg     1500

cccuggagga guucaacaag caccgcgaca ucgacaagca gugccgguuc gaggagaucc     1560

uggccaacuu cgccgccauc cccaugaucu ucgacgagau cgcgcagaac aaggacaacc     1620

ucgcccagau cagcaucaag uaccagaacc agggcaagaa ggaccugcug caggccuccg     1680

ccgaggacga cgucaaggcc aucaaggacc uccuggacca gaccaacaac cugcugcaca     1740

agcucaagau cuuccacauc agccaguccg aggacaaggc gaacauccug gacaaggacg     1800

agcacuucua ccugguguuc gaggagugcu acuucgagcu cgccaacauc gugccgcugu     1860

acaacaagau ccgcaacuac aucacccaga agcccuacag cgacgagaag uucaagcuga     1920

acuucgagaa cagcacccuc gccaacgggu gggacaagaa caaggagccc gacaacacgg     1980

ccauccuguu caucaaggac gacaaguacu accugggcgu caugaacaag aagaacaaca     2040

agaucuucga cgacaaggcg aucaaggaga acaaggggga gggcuacaag aagaucgugu     2100

acaagcuccu gcccggcgcc aacaagaugc ugccgaaggu guucuucucc gccaagagca     2160

ucaaguucua caaccccucc gaggacaucc uccggauccg caaccacagc acccacacca     2220

agaacgggag cccccagaag ggcuacgaga aguucgaguu caacaucgag gacugccgga     2280

aguucaucga cuucuacaag cagagcaucu ccaagcaccc cgaguggaag gacuucgggu     2340

uccgcuucag cgacacccag cgguacaacu ccaucgacga guucuaccgc gaggucgaga     2400

accagggcua caagcugacg uucgagaaca ucagcgaguc cuacaucgac agcgugguca     2460

accaggggaa gcuguaccuc uuccagaucu acaacaagga cuucuccgcc uacagcaagg     2520

gccggccgaa ccugcacacc cuguacugga aggcccucuu cgacgagcgc aaccugcagg     2580

acguggugua caagcugaac ggcgaggcgg agcuguucua ccggaagcag uccaucccca     2640

agaagaucac ccaccccgcc aaggaggcca ucgccaacaa gaacaaggac aaccccaaga     2700

aggagagcgu cuucgaguac gaccucauca aggacaagcg cuucaccgag gacaaguucu     2760

ucuuccacug cccgaucacg aucaacuuca aguccagcgg ggccaacaag uucaacgacg     2820

agaucaaccu gcuccugaag gagaaggcga acgacgugca cauccugagc aucgaccggg     2880

gcgagcgcca ccucgccuac uacacccugg uggacgggaa gggcaacauc aucaagcagg     2940

acaccuucaa caucaucggg aacgaccgca ugaagaccaa cuaccacgac aagcuggccg     3000

ccaucgagaa ggaccgggac uccgcccgca aggacuggaa gaagaucaac aacaucaagg     3060

agaugaagga gggcuaccuc agccaggucg ugcacgagau cgcgaagcug gugaucgagu     3120

acaacgccau cgucguguuc gaggaccuga acuucggcuu caagcggggg cgcuucaagg     3180

uggagaagca ggucuaccag aagcucgaga agaugcugau cgagaagcug aacuaccucg     3240

uguucaagga caacgaguuc gacaagacgg gcggggugcu gcgggccuac cagcugaccg     3300

cccccuucga gaccuucaag aagaugggca agcagaccgg gaucaucuac uacguccccg     3360

cgggcuucac cuccaagauc ugccccguga cgggcuucgu gaaccagcuc uacccgaagu     3420

acgagagcgu cuccaagagc caggaguucu ucuccaaguu cgacaagauc ugcuacaacc     3480

uggacaaggg guacuucgag uucagcuucg acuacaagaa cuucggcgac aaggccgcca     3540

aggggaagug gaccaucgcc uccuucggca gccgccugau caacuuccgg aacuccgaca     3600

agaaccacaa cugggacacc cgcgaggugu accccaccaa ggagcucgag aagcugcuga     3660

aggacuacag caucgaguac ggccacgggg agugcaucaa ggccgcgauc ugcggcgagu     3720

ccgacaagaa guucuucgcc aagcucacga gcgugcugaa caccauccug cagaugcgga     3780

acuccaagac cgggaccgag cucgacuacc ugaucagccc cgucgccgac gugaacggca     3840

acuucuucga cucccgccag gcccccaaga acaugccgca ggacgccgac gcgaacgggg     3900

ccuaccacau cggccugaag ggccucaugc ugcuggggcg gaucaagaac aaccaggagg     3960

gcaagaagcu caaccuggug aucaagaacg aggaguacuu cgaguucguc cagaaccgca     4020

acaacaagcg gcccgccgcc acgaagaagg ccgggcaggc gaagaagaag aaguaaggac     4080

uaguguccac cugucccucc ugggcugcug gauugucucg uuuuccugcc aaauaaacag     4140

gaucagcgcu uuacagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       4229


<210>  2538
<211>  51
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS2 variant

<400>  2538
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc a                51


<210>  2539
<211>  48
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS4 variant

<400>  2539
aagagacccg ccgcuaccaa aaaggcaggc caggccaaaa agaaaaag                    48


<210>  2540
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2540
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg aguauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugauaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug acagugggga aacggcagag      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggaucugc      240

uaucugcagg aaauuuucag caacgagaug gcuaaaguag augacuccuu uuuccaucgc      300

cuagaagagu cuuuucucgu ggaggaagau aagaaacacg agagacaucc uaucuucgga      360

aauauagucg acgaaguggc cuaccacgag aaguauccaa ccaucuacca ucugaggaag      420

aaacuuguug auucgacaga caaggcagau cugcgguuga uuuaucucgc ccuggcucac      480

augaucaaau uucgcggcca cuuccugauu gagggugacc ucaacccgga uaauucagac      540

guggacaagu uauuuaucca acugguccag accuacaacc agcuuuucga agagaauccc      600

auaaacgcaa gcggggugga ugccaaagcu auuuugagug cccgacuguc caagucuaga      660

aggcuagaaa aucucaucgc gcagcugccu ggagagaaaa agaacggccu uuuuggcaau      720

cugauugcau ugagccucgg gcugacucca aacuucaagu ccaauuuuga cuuagccgaa      780

gaugcuaaac ugcaacucuc aaaggacaca uacgaugacg aucuugacaa ccuguuggcc      840

cagaucggag aucaguaugc agaccuauuc cuggcugcca aaaaccucag ugaugcgauc      900

cugcuuagcg acauucugcg gguaaauacc gagauaacga aggcccccuu gucugcaucc      960

augaucaaac guuacgacga gcaucaccag gaucucacuc uguuaaaggc ucugguucgc     1020

caacagcuac cugaaaagua uaaagagauu uucuuugacc agucaaagaa cgguuacgcc     1080

ggcuauaucg augggggagc uagccaggaa gaguucuaca aauuuauaaa gccaauucuc     1140

gaaaagaugg acggcaccga ggagcugcuu gugaaguuga auagagaaga ucugcucagg     1200

aagcaacgga cauucgacaa cgguaguauc ccccaucaga uccaccuggg ggagcuucac     1260

gccauucugc gacgccagga agauuuuuau ccuuucuuga aagacaauag agagaagauc     1320

gagaaaauuc ucacuuuuag gauaccauac uacgucggac cgcuggcacg gggcaacucu     1380

cgcuucgccu ggaugaccag aaaguccgaa gagacaauca cccccuggaa uuuugaagag     1440

gugguggaua aaggcgcuag cgcgcaaucg uucauugaaa ggaugacgaa cuuugacaag     1500

aauuuaccua acgagaaggu ccugccaaaa cauucucuac ucuaugagua cuucacuguu     1560

uauaaugaac ugacaaaggu gaaauacgua accgagggga ugcguaagcc cgcauuucuu     1620

uccggagaac agaaaaaggc caucguggau uugcuguuca aaacuaaccg gaaggucaca     1680

gugaagcagc ucaaagagga cuauuucaag aaaaucgaau guuuugauuc aguugagauu     1740

aguggugugg aggaccgauu caaugcuagc cugggcaccu accacgacuu acugaagaua     1800

aucaaagaua aggacuuucu ugauaacgaa gagaaugaag acauuuugga ggauaucguc     1860

cucacgcuga cccuauucga agacagagag augauugagg aaaggcugaa gacauaugcc     1920

caucucuuug augacaaagu gaugaagcag cugaaacgcc ggagauacac ugggugggga     1980

aggcuuuccc gcaaguugau caacggcaua cgagauaaac aaucugguaa gaccauccug     2040

gacuucuuaa agagcgaugg guuugcaaac cggaauuuca ugcagcucau ucacgacgau     2100

ucacugacau uuaaagagga cauccagaag gcccagguaa guggccaagg agacucccuu     2160

caugaacaca uugcuaaccu ggccggcagc ccugcaauca aaaaggggau auugcagacu     2220

guuaaagugg ucgaugagcu cgugaagguc augggacguc acaagccaga aaauauugug     2280

aucgagaugg cgagagagaa ccagaccaca cagaaagguc aaaagaacuc uagggaacgc     2340

augaaacgga uugaggaagg caucaaggag cuggggucac agauacuaaa agagcauccc     2400

guugaaaaua cccagcugca gaaugagaag cucuaccugu auuaccuuca aaacggaaga     2460

gacauguaug uggaucagga auuggacauc aauaggcuga gugauuacga cguagaucac     2520

auugucccgc agagcuucuu aaaagacgau uccaucgaca acaaggugcu cacucgaucu     2580

gauaagaauc gcggcaaauc ggacaacguu ccuagcgagg aaguggucaa gaaaaugaag     2640

aauuauuggc ggcaacugcu uaacgcuaaa cugauuacgc agagaaaguu ugacaaucua     2700

acaaaggccg agaggggugg gcucuccgag cuggauaaag caggcuucau caagcgucag     2760

uugguggaaa cccgccagau aacuaaacau guagcccaaa uucuggacuc acggaugaac     2820

accaaguacg augagaacga caaauuaauc agagaaguga agguuaucac acucaagagu     2880

aaacuggucu cugauuuuag gaaggacuuc caguucuaua aagugcgaga gauuaauaac     2940

uaccaccacg cucaugaugc cuaccuuaau gcaguggucg gaaccgcucu gaucaagaaa     3000

uaucccaagu uggaaagcga guuuguuuac ggcgacuaua agguguacga ugugcggaaa     3060

augauugcca aguccgagca ggaaauaggg aaagcgacug ccaaguauuu cuuuuacuca     3120

aacaucauga auuucuuuaa aacagagauu acgcucgcua acggagaaau ccgcaagaga     3180

ccacugauug agaccaaugg ugaaacuggc gagaucguau gggacaaggg gagggauuuc     3240

gcaacagucc ggaaagugcu aagcaugccu cagguuaaca uagugaagaa aaccgagguc     3300

caaacaggag gcuuuucuaa ggaauccauc cugcccaaac gaaauaguga caaacuuauu     3360

gcccgcaaaa aggauuggga cccaaagaaa uauggugggu ucgacagccc cacuguggcu     3420

uacucuguuc ucgugguagc caaggucgag aaaggcaagu ccaaaaagcu gaagucagug     3480

aaagaauugc ugggaaucac cauuauggag agaaguagcu uugagaagaa cccuaucgau     3540

uucuuagaag caaaaggcua caaggaggug aaaaaggacc ucauaauuaa gcugccaaaa     3600

uauucgcuuu uugaacugga gaaugggagg aagcguaugu uggcguccgc cggagaacuc     3660

cagaaaggca acgagcuggc ucuaccgucu aaguacguca auuuccugua ucuugccagc     3720

cacuacgaga aacucaaggg uucacccgaa gauaacgagc agaagcagcu guucguugaa     3780

caacauaaac acuauuugga cgagaucauc gaacagauua gugaguuuuc caagcgggug     3840

auauuagcag augcuaaucu ggacaaagua cucucugccu acaacaagca uagagauaaa     3900

ccuaucaggg agcaggcaga aaacauuauc caccuguuca cgcuuaccaa ucugggggcc     3960

ccagcugcgu uuaaguauuu cgacacaacu auugaucgca aacgauacac cagcacaaag     4020

gagguguugg acgccacucu aauccaccaa uccauaaccg gacuguacga aacgagaauc     4080

gaucucucac agcugggcgg ugacuga                                         4107


<210>  2541
<211>  4890
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2541
augaacuuca agauccugcc cauugccaua gaccucggcg ugaaaaauac cggggucuuu       60

agcgcuuucu accagaaggg aacaucccuu gagagacugg auaacaaaaa ugguaagguu      120

uaugaauugu cuaaagacuc auacacuuua cugaugaaca auaggacggc acggcgccac      180

caacgacgug gcaucgauag aaagcagcua gugaagaggc ucuuuaaacu gauuuggacc      240

gagcagcuua accuggaaug ggacaaggau acacagcaag ccaucaguuu cuuguuuaau      300

cggcgcgggu ucagcuuuau uacugacgga uauuccccug aguaccucaa caucguacca      360

gaacagguga aagcgauacu gauggauauc uucgacgauu auaauggcga ggacgaucug      420

gacucuuacc ucaaguuagc uaccgagcag gaaucgaaaa uuucagagau cuauaacaag      480

cugaugcaga agauucuuga auuuaaauug augaagcugu gcacagauau caaagacgac      540

aaggucagca ccaaaacucu aaaggagaua acaaguuacg aguucgaacu ccuggccgau      600

uaccuugcaa auuauuccga gucucugaaa acccaaaagu uuagcuacac ggacaagcag      660

gguaacuuga aagaacucuc cuauuaccau cacgauaagu auaauauuca ggaguuccug      720

aaaagacaug ccacuaucaa cgacaggauu uuagauaccc ugcucacaga cgaucuugac      780

aucuggaauu uuaacuucga aaaguucgau uuugacaaaa acgaggagaa gcugcagaau      840

caagaagaua aggaccacau ccaggcucau uugcaccacu ucguguuugc aguuaacaaa      900

auuaagucag agauggccag ugggggacgg caucgcagcc aguacuucca agaaauaacu      960

aaugugcuag acgagaacaa ucaccaggaa ggcuaucuga aaaacuuuug ugagaaucuc     1020

cauaacaaga aauacucuaa ucuguccguc aagaaccuug ugaaucugau cggcaacuug     1080

ucaaaucucg agcugaagcc guuacgaaaa uacuucaacg auaagauuca cgcuaaagcc     1140

gaccacuggg augaacagaa guuuaccgag acauauugcc auuggauccu gggggaaugg     1200

agaguaggag uuaaagacca ggauaagaag gacggugcga aauacagcua uaaggaucua     1260

uguaaugagc ucaaacaaaa ggugaccaaa gcaggccugg ucgacuuccu uuuggaacug     1320

gaucccugca ggacgauacc uccauaccuc gacaacaaca aucggaagcc cccuaaaugu     1380

cagagucuga uucuuaaccc aaaguuucug gauaaucagu aucccaacug gcagcaauac     1440

uugcaggagc ucaagaaacu gcagucuauc cagaauuauu uagacuccuu cgagacugau     1500

cugaaggugc uaaaaagcuc gaaggaccaa ccuuacuuug uggaauacaa aucuuccaac     1560

cagcagaucg ccucagggca gcgugacuau aaggaucucg acgcucgcau ucugcaauuc     1620

aucuuugaua gagucaaggc cagugacgag cuuuugcuga augaaauuua cuuccaggcu     1680

aaaaagcuca aacagaaggc uagcuccgag cuggagaaau uagaaucuag caagaagcug     1740

gaugagguua uagccaacuc acaacuuagu cagaucuuga aaucccagca cacaaaugga     1800

auuuucgaac agggcaccuu ucuccaucug gugugcaagu auuacaaaca aaggcagcgg     1860

gcgcgagaca gccgccuaua uaucaugcca gaguacagau augauaagaa acugcacaag     1920

uacaacaaua cugguagguu cgacgaugac aaccagcucc ugacauacug uaaucauaag     1980

ccgcggcaga aacgcuauca acuuuugaac gaucuggccg ggguauuaca ggugucuccc     2040

aauuuucuca aggacaaaau cggaucagau gacgaccugu ucauuaguaa guggcuuguc     2100

gaacacauaa gaggcuuuaa aaaggcaugc gaggauagcc ugaaaaucca gaaggacaac     2160

aggggcuugc ucaaccacaa gauuaauauc gcucguaaca ccaaagggaa gugugagaaa     2220

gaaauuuuca aucugaucug caagauagag ggauccgaag auaaaaaggg uaacuacaag     2280

cauggccuag ccuaugagcu gggggugcuc cuguuuggag aaccuaauga ggcuucuaaa     2340

ccagaguucg accggaagau caaaaaguuu aacucgauuu acagcuucgc ccagauccaa     2400

cagauugcau uugccgaacg aaaaggcaau gcuaacacgu gugcgguuug cuccgcagau     2460

aaugcccaca gaaugcagca aaucaagaua accgagcccg uggaagacaa caaggauaaa     2520

auuauccuuu cagcuaaggc ccagagguug ccugcaauuc caacacgcau cgucgacggu     2580

gccgugaaaa agauggcuac uauacuggcc aaaaauaucg uagaugacaa cuggcagaau     2640

auuaagcagg uuuuaagugc aaagcaucaa cuccacaucc ccauuaucac cgagucuaac     2700

gcguucgaau uugagccggc ucuggccgau gugaaaggga aaagccuuaa agaccggaga     2760

aagaaagcac uggagaggau auccccugaa aauauuuuca aggacaagaa caaccgcauc     2820

aaagaguucg ccaagggcau cucagcuuau agcggagcca aucuaacaga uggcgacuuu     2880

gauggggcaa aagaagaacu cgaccacauu aucccccgau cucauaagaa auacggaacu     2940

cugaacgaug aggcuaauuu gauuugcguc acccggggug acaacaagaa uaaaggcaac     3000

cguauauucu gucugagaga uuuagccgac aauuauaagc ucaagcaguu ugaaacaacc     3060

gaugaccugg agaucgaaaa gaagauugcg gauacuaucu gggacgccaa caaaaaggau     3120

uucaaauuug ggaauuacag guccuucauu aaccuuacgc cacaggagca gaaggcuuuu     3180

cgccacgcac uguucuuggc cgacgaaaau ccuaucaagc aagcugugau acgggccauc     3240

aacaauagaa acaggacauu ugucaaugga acccagcgau acuucgcaga ggugcucgcg     3300

aacaauauuu aucugcgcgc caaaaaggag aaccuaaaca cugacaaaau caguuuugau     3360

uacuucggca uucccaccau cgguaauggg cggggcauag cugaaauuag acagcuguau     3420

gagaagguug acagcgauau ccaggccuac gcaaaaggag acaagccaca agcaucuuau     3480

ucccaucuua ucgaugccau gcucgcauuc ugcauugccg cugacgaaca caggaacgau     3540

ggcucaauag ggcuggagau cgacaagaau uacaguuugu auccccugga uaaaaacaca     3600

ggagaagugu uuaccaagga cauuuucagc cagaucaaaa uuacugauaa ugaguuuucg     3660

gacaagaaau uaguacguaa gaaggcgauc gaggguuuca acacacaucg ccagaugacg     3720

cgggauggca uauacgccga aaauuaccuc ccuauccuga uucacaaaga gcuuaacgaa     3780

gucagaaagg gguauaccug gaaaaauucc gaggaaauca agauuuuuaa aggaaagaaa     3840

uacgacaucc agcaacugaa caauuuggug uauugucuca aguucguuga caagccaaua     3900

ucuauugaua uccagaucag cacucuggag gagcuaagga acauucugac aaccaauaac     3960

aucgcagcua cagccgaaua cuauuacaua aaucuuaaaa cucagaagcu ccacgaguau     4020

uacauugaaa acuacaacac cgcccugggc uauaaaaagu acucaaaaga gauggaguuu     4080

uugcgaaguu uagcuuaucg guccgaacgc gugaagauca agucuauuga cgaugucaaa     4140

caagugcugg acaaggauag caauuucauc auagguaaaa uuacgcuccc guuuaagaaa     4200

gaguggcaga gacuguacag ggaauggcag aacaccacaa ucaaggacga uuaugaguuc     4260

cuuaaguccu uuuucaaugu aaaaucaauc acuaagcugc auaaaaaggu gcggaaagac     4320

uuuagcuugc ccauuaguac caacgaaggg aaguuccuag uuaagcgaaa aacaugggau     4380

aauaacuuca ucuaccagau ucugaaugac ucugauucca gagcagacgg cacuaagccu     4440

uuuauaccag ccuucgacau cagcaaaaac gagauugucg aggcgaucau cgauucguuu     4500

accucaaaga auauuuucug gcuccccaaa aacauagaac ugcaaaaggu ggacaauaaa     4560

aacaucuuug cuauugauac gucuaagugg uucgaggugg aaacaccuag ugaccuucgc     4620

gauaucggaa uugccaccau ccaguauaag auagacaaua acuccaggcc aaaaguccgu     4680

guuaaguuag auuacgugau cgacgaugac agcaaaauua auuacuuuau gaaccacucu     4740

cugcucaaga gccgguaucc cgauaaagug uuggagaucc ugaagcaguc cacuauuauc     4800

gaguucgagu caaguggcuu uaacaagaca auaaaagaga ugcucgggau gaagcuggca     4860

ggaauuuaca augaaaccag caacaauuga                                      4890


<210>  2542
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2542
augaagagaa acuacauccu gggccucgac auugggauaa ccagcguggg auaugguauc       60

auugauuacg agacaaggga cgucaucgau gccggcguuc ggcuuuucaa agaagcuaau      120

guggagaaca augaagggcg ccgauccaag cguggagcaa gaaggcugaa acggcgcaga      180

aggcaccgga uucagcgcgu aaagaaauug uuauuugacu auaaccugcu aacugaucau      240

ucugagcucu caggcaucaa ucccuacgaa gcccgaguga agggucugag ucaaaaacuu      300

agcgaggagg aguucuccgc ggcucuguug caccucgcca aaagaagggg aguccauaac      360

gugaaugaag uugaagagga cacggggaac gagcugucua ccaaggaaca gauaucgcgu      420

aauucaaaag cacuggagga aaaguaugug gccgagcucc aguuagaacg gcugaaaaag      480

gauggcgagg uccgcggcag caucaacaga uuuaagacaa gugacuacgu gaaagaggcu      540

aagcagcuuu ugaaaguaca aaaggcauau caccagcugg aucaguccuu cauugacacu      600

uacaucgauc uacucgaaac caggcggaca uacuaugagg ggccuggaga agguucucca      660

uuuggcugga aagacauuaa ggagugguac gaaaugcuga uggggcauug caccuauuuc      720

ccggaggagc uucgaagcgu uaaauacgcc uauaaugcug aucuguacaa cgccuugaau      780

gaccucaaca aucuggugau cacucgcgac gaaaacgaga aguuagaaua uuacgagaag      840

uuucagauaa uugaaaacgu cuucaaacaa aagaaaaagc ccacacugaa acagaucgcg      900

aaggagauuc ucgugaauga ggaagauauc aagggauaca gagugaccuc cacgggcaaa      960

ccugaguuua cuaaccuuaa ggucuaucac gacaucaaag auauuaccgc aaggaaggaa     1020

auaaucgaga augccgagcu guuggaccag auugcuaaaa uccuaacaau auaccaguca     1080

agugaagaua uucaagagga acugacuaac cucaauagcg agcugaccca ggaagagauc     1140

gagcagaucu cuaaccuuaa ggguuauaca gggacccaca aucuguccuu gaaggccauu     1200

aaccucaucc uggacgaauu auggcauacg aaugauaacc aaauugcaau auucaaucgg     1260

cugaagcuag uuccaaagaa aguggaccuc ucacagcaga aggagauccc cacuacacug     1320

guagaugacu uuauucuuag cccugugguc aaacgcaguu ucauccaguc uaucaaggug     1380

auuaacgcua uaaucaagaa auacggauug ccaaaugaua uuaucauuga acuggccaga     1440

gagaagaacu ccaaagacgc gcaaaagaug aucaaugaaa ugcagaaaag gaaccgucag     1500

accaacgagc ggauagagga aaucauucga acuacaggca aggagaaugc caaauaucuc     1560

aucgaaaaga uuaagcugca cgacaugcag gagggcaaau gucuuuacag ccuggaagca     1620

auccccuugg aggaucuccu gaacaauccu uuuaacuaug agguugacca uauaauucca     1680

agaucggugu cuuucgauaa uuccuucaac aauaaggucu uagugaaaca agaagagaac     1740

ucaaagaaag ggaauaggac cccguuucag uaccugagua gcuccgacuc uaagaucagc     1800

uacgaaacgu ucaagaaaca cauucuaaac cucgcuaagg gaaaaggucg caucucaaag     1860

accaaaaagg aguaucugcu ugaggaacgg gauauaaaua gauuuagugu acagaaggac     1920

uucaucaaca ggaauuuggu ugauacacgc uacgccacuc gaggccugau gaaccuccug     1980

cgguccuauu uucgugugaa uaacuuagac gucaaaguga aaagcauuaa cgggggauuc     2040

accucuuuuc ugagaaggaa auggaaguuc aaaaaggagc gcaauaaggg cuacaaacac     2100

caugcugaag augcccuuau cauugcaaac gccgacuuua ucuucaagga guggaaaaag     2160

uuggauaaag cuaagaaagu cauggaaaau cagauguuug aggagaagca agcggaauca     2220

augcccgaga uagaaacaga gcaggaauau aaggagauuu ucaucacucc ucaccagauc     2280

aaacauauua aggacuuuaa agauuacaag uauagucacc ggguggacaa aaagccaaac     2340

agagagcuca ucaaugacac ccuguacagc acaaggaagg augacaaagg uaacacccua     2400

auuguuaaua accugaaugg gcucuacgau aaggacaacg auaaacugaa gaaacuuaua     2460

aauaaguccc ccgaaaaguu gcugauguau caucacgacc cucagacuua ccaaaaauua     2520

aagcucauca uggagcagua uggcgaugag aaaaacccac uguacaagua uuacgaagaa     2580

acgggaaauu aucuuacaaa auacucuaag aaggacaacg gccccgugau uaaaaagauc     2640

aaauacuaug ggaauaagcu gaacgcacac uuggauauua ccgacgauua cccgaacucg     2700

cgaaauaaag uagucaagcu cagccugaag ccuuaucgcu ucgacgugua ccuagauaac     2760

ggaguuuaua aauucgugac ugucaagaau cuggacguga ucaaaaagga gaacuacuau     2820

gagguaaauu ccaaaugcua cgaagaggcc aagaaacuca agaagauauc aaaccaggcu     2880

gaauuuaucg ccaguuucua caauaacgac cugauuaaaa ucaaugguga gcuuuaucgg     2940

gugauuggcg uuaacaauga uuugcugaac agaaucgagg ucaauaugau agacauuacc     3000

uacagggaau auuuagagaa caugaaugau aagcgucccc cacgcaucau caaaacaauu     3060

gcaucuaaga cccaaagcau caaaaaguac uccacugaca uacucgggaa ccuguaugaa     3120

gugaagucaa agaagcaucc ucagauuauc aaaaagggau ga                        3162


<210>  2543
<211>  4230
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2543
augcuguuca acaagugcau cauuauaagc aucaaucucg acuuuuccaa caaagagaag       60

uguaugacca aacccuacuc uauuggccuu gauaucggga caaauucagu gggaugggcc      120

gucauuacug acaacuauaa gguuccuagu aaaaagauga aggugcuggg uaauacgagc      180

aaaaaguaca ucaaaaagaa cuuguuaggc guacugcuau ucgauuccgg gauaaccgcu      240

gaaggaagaa ggcucaaacg gacagcacgc cgacguuaua cuagaaggcg gaaucgcauc      300

cuguaccuuc aggagauuuu uaguaccgaa auggccacac uggacgaugc guucuuucaa      360

agauuggacg auucguuccu cgugccagac gauaagaggg acucaaagua uccgaucuuu      420

ggcaaccugg ucgaggaaaa aguguaccac gaugaguucc ccaccauuua ucaucugcgg      480

aaguaccucg cugacagcac uaaaaaggcc gauuuacgcc ugguuuaccu ugcauuggcc      540

cacaugauca aauaucgagg ucauuuucug auagaggggg aguucaauag uaagaacaau      600

gacauucaga aaaacuuuca ggacuuccua gauacauaca augcuaucuu ugaauccgac      660

cucucucugg aaaacagcaa gcagcuugag gagauuguga aggauaaaau cuccaagcug      720

gaaaagaagg acagaaucuu gaaacucuuc ccuggagaga aaaauucagg cauuuucagu      780

gaauuucuga aguuaauagu cggcaaccaa gcagauuuca ggaaaugcuu uaaccuggac      840

gagaaggcca gccuccacuu cucuaaagaa uccuaugaug aggaccuuga gacccuguug      900

ggguacaucg gagaugacua uucagaugug uuucuaaagg cuaaaaagcu guacgacgcc      960

auucuccuga gcgguuuccu uacgguaacu gacaaugaaa ccgaggcgcc acugaguucu     1020

gcaaugauca agcgguauaa cgaacauaaa gaggauuugg cccuccugaa ggaauacaua     1080

cguaauauuu ccuuaaaaac auacaacgag guuuuuaagg acgauacuaa aaauggcuau     1140

gcuggguaca ucgacggaaa gaccaaccag gaggauuucu auguguaccu gaagaaucua     1200

cucgccgaau uugagggcgc agacuauuuc cuggaaaaga ucgaucgcga ggacuuucuu     1260

agaaagcaga ggacauucga uaacgguagc auucccuacc agauccacuu gcaagaaaug     1320

cgggcuauuc uggacaaaca ggccaaguuu uauccuuucc ucgcgaaaaa uaaggagcga     1380

auagagaaaa uccugaccuu ccgcauucca uacuacgucg ggccccuugc cagaggaaac     1440

ucggauuuug cauggucuau caggaagcgg aaugaaaaga ucacgccuug gaacuucgag     1500

gacgugauug auaaagaauc cucagcugag gccuuuauaa aucgcaugac uucuuucgac     1560

cuguauuugc cagaggaaaa ggugcucccg aaacacagcc uguuauacga gacauuuaac     1620

gucuauaaug aacugaccaa gguuagauuc aucgcugagu ccaugaggga cuaccaguuu     1680

cuagauucua aacagaagaa ggacauugug cgucucuauu ucaaagauaa gcggaaagua     1740

acugacaagg auaucauuga auaccugcau gccaucuaug gcuacgacgg cauagagcuu     1800

aaagggaucg agaagcaauu uaacagcuca uugaguacau accacgaucu gcucaacauu     1860

aucaaugaca aggaguuccu ggaugacucc agcaacgaag caauuaucga agagauaauu     1920

cauaccuuaa cgaucuuuga agaucgagag augauuaaac agagacuguc uaaguucgag     1980

aauaucuuug acaaaucagu gcuuaagaaa uugaguaggc gccacuauac cggauggggu     2040

aagcucagcg ccaagcugau aaacggcauc cgggaugaaa aauccgggaa uacaauucua     2100

gacuaccuga ucgacgaugg aauuucuaac agaaauuuca ugcagcucau ccacgacgau     2160

gcucugucgu ucaagaaaaa gauacaaaaa gcgcagauua ucggcgacga ggauaagggu     2220

aacaucaaag aagucguuaa gagccuuccc ggguccccug caauuaagaa aggcaucuug     2280

cagucaauua agauagugga cgagcuggug aaagucaugg gaggcaggaa gccagaaagu     2340

aucgugguag agauggcccg cgagaaucag uauacuaacc aagggaaauc uaauagccag     2400

cagcgauuaa agcggcucga aaagucccug aaagagcuug gaucaaagau ucugaaagaa     2460

aacauccccg cuaaguugag caaaauugau aauaacgccc uccagaauga ccgucuguac     2520

cuauauuacc ugcaaaacgg uaaggauaug uauaccggcg acgaucucga caucgacaga     2580

cugucuaacu acgauauaga ccauaucauu ccucaggcau uucuuaagga uaauuccauc     2640

gacaacaaag uuuuggugag uagcgccucu aauaggggga aguccgauga cuucccauca     2700

cuggaggucg ugaaaaagcg caaaacauuu ugguaccagu uacucaagag uaagcugauu     2760

agccagcgga aauucgauaa ccuuacuaag gcugagagag gaggccugcu acccgaagac     2820

aaagccgguu uuauccaaag gcagcucguc gagacccgac agauaacaaa gcacguggca     2880

cgccuguugg augaaaaauu caauaacaag aaggacgaga auaaccgggc gguuagaacc     2940

gugaaaauua ucacucugaa gucgacguua guaucccagu uuaggaaaga uuucgaacuc     3000

uauaaggucc gugagaucaa ugacuuucau cacgcucaug augccuaccu gaacgcagug     3060

auugccucug cccuucugaa aaaguauccg aaauuggagc cugaguucgu uuacggggac     3120

uaucccaagu acaauagcuu ucgcgaacgg aagucagcua cagaaaaagu guauuucuac     3180

aguaacauaa ugaauaucuu caagaaaucc auuucucucg cagacggcag agucaucgag     3240

aggccacuga uugaagugaa cgaggagacc ggagaaagcg uauggaauaa ggaguccgau     3300

cuagcuacug ugcgacgggu ucugucauac ccucaaguca acguggugaa aaaggucgag     3360

gaacagaacc acggccuuga ccgcgggaag cccaaaggac ucuuuaaugc caaccugagc     3420

aguaagccaa aacccaauuc uaacgaaaau uugguuggug cgaaggagua ucuggauccu     3480

aaaaaguacg gcggguaugc cggaaucucc aacagcuucg cuguguuagu gaagggcacc     3540

auagagaaag gugcaaagaa aaagaucaca aauguacucg aauuucaggg gauuucgauc     3600

cuggacagaa uuaacuacag gaaagauaaa cuuaauuucc uguuggagaa gggcuauaaa     3660

gacaucgaac ucauaauuga gcugccaaag uacucacuau uugagcuguc ugauggaagu     3720

cggcgaaugc uugccuccau ccucagcacc aacaauaaaa gaggcgaaau ccacaagggg     3780

aaccaaauuu uccugucuca gaaauuuguc aaguuguuau aucaugcuaa acgcaucagc     3840

aauacuauaa acgagaauca caggaaguac guggaaaacc auaagaaaga guucgaggaa     3900

cuguuuuacu auauucucga guucaaugaa aacuacguug gagccaagaa aaacggcaag     3960

cugcuuaauu ccgcauuuca gucauggcag aaccacagua ucgacgagcu guguagcucu     4020

uucauugguc cgacaggguc cgaacguaaa ggauuguuug agcuaacguc acggggcagu     4080

gcggccgauu ucgaguuccu gggugugaag auccccagau auagggacua caccccuagc     4140

ucccuccuga aggaugcuac ucuuauacac caaucuguca caggcuuaua ugaaacccgc     4200

auugaccugg ccaaacucgg ggagggauga                                      4230


<210>  2544
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2544
augugguacg ccagccugau guccgcucac caucucagag ugggcaucga cgucgggacc       60

cacucuguug gacuugcaac acugagggug gaugaccaug guacucccau ugaguuguua      120

ucagcccuga gucacauaca ugauagcggc guagggaagg aaggaaagaa ggaccacgau      180

acgcggaaaa agcuauccgg caucgcgcgc cgagcucgua gacuccugca ccauaggcgg      240

acccagcuuc aacagcugga cgagguguug cgcgaucucg guuucccuau uccaacaccg      300

ggggaauuuc uggaccugaa cgagcagacu gaucccuaua gagucuggag ggugcgggcc      360

cgccucguug aagagaaauu accugaggaa cugcgaggac cagcaaucuc uauggccgug      420

agacacauug cuaggcaucg gggcuggcgu aaucccuacu cgaaggucga gucacuuuug      480

agcccugcag aagagagucc auucaugaag gcccugcgcg agagaauccu agcuaccaca      540

ggcgaagugc ucgacgaugg gauaaccccc ggacaggcca uggcgcaagu agcacugacu      600

cacaacaucu ccaugagggg uccugagggc auucuuggga aacugcacca gucugacaau      660

gccaacgaaa uccggaagau uugcgcucga cagggaguua gcccagaugu guguaaacag      720

uugcuccgcg ccgucuuuaa ggcagacucc ccgagaggcu cagcugugag uaggguggcc      780

cccgacccuc ugccagguca agggagcuuc cggcgcgcgc ccaaaugcga uccugaguuu      840

cagagauuca ggaucauauc uauugucgcc aauuuacgua ucuccgaaac aaagggagag      900

aaccggccac ugaccgcaga cgagcgaaga cauguuguga cguuucucac ugaagauuca      960

caggcugacc uuaccugggu agauguggcc gagaagcugg gcguccacag gcgcgacuug     1020

cggggcacag cugugcauac ugaugacggg gaaagaagcg ccgcaaggcc cccgauugau     1080

gccaccgacc gcaucaugcg acagacaaaa aucaguucuc uaaagaccug gugggaggaa     1140

gcugauuccg agcaacgggg agcgaugauu cguuaucugu acgaggaccc uacggacagc     1200

gaaugugcag agauaaucgc cgaacucccc gaggaagauc aggcuaaacu ggacucgcuu     1260

caucugccag ccgguagagc agccuauucu agggaguccu ugacugcucu cucagaucac     1320

augcuggcca caaccgacga uuuacacgag gcacgcaagc ggcuguucgg cguugacgau     1380

aguugggcgc cucccgcuga agccauuaau gcaccagugg ggaaccccag cgucgacaga     1440

acucuaaaaa ucgugggaag guaccucucc gccguagagu cuaugugggg cacaccugaa     1500

guuauacacg uggagcaugu ccgagauggu uuuaccagcg agcgcauggc ugacgaacgg     1560

gauaaggcca auagaaggcg uuauaacgac aaucaggagg caaugaaaaa gauucaacgc     1620

gauuacggga aggaaggcua caucucacgg ggagacaucg ugagacugga cgcucuugag     1680

uugcagggcu gcgccugucu guauugcggg acgaccauug gauaccacac augucagcuc     1740

gaucauaucg ucccacaggc ggguccgggc aguaacaaua ggcgagggaa ccugguggcc     1800

guuugcgaac gguguaaucg cuccaaaagc aacacucccu ucgcugugug ggcacaaaag     1860

ugcggcauuc cucacguagg agucaaagag gccauaggua gagugagggg guggcggaag     1920

cagaccccaa acacaucuuc agaggaccuu acucgacuga aaaaggaagu uaucgcuaga     1980

uugcgcagga cccaggagga ucccgaaauu gacgagcgua guauggaaag cguggccugg     2040

auggcaaaug agcuccacca ucggaucgcg gccgcuuauc cugagacaac cgucauggug     2100

uacagaggcu ccaucacugc cgcagcuagg aaggccgcag gaauugauuc ucgcauaaac     2160

cugaucggcg aaaaagggcg aaaggacaga auugaucgga ggcaccaugc cguagacgcu     2220

ucggugguug cguuaaugga ggccagcguc gcaaaaacgc uggcugaacg cuccucacua     2280

cguggagagc agagacucac agguaaggaa caaaccugga aacaguauac uggcaguacc     2340

gugggggccc gggagcacuu ugagaugugg aggggacaua ugcugcaccu uacagaauug     2400

uucaaugagc gccuggccga agauaaggug uacgucaccc agaacauccg acucagacug     2460

ucugacggca augcucacac uguuaaccca agcaaguuag ugucccaucg gcugggugau     2520

gggcuuacag ugcagcaaau ugacagggca uguacgcccg ccuuguggug cgcgcucacc     2580

cgcgagaaag auuuugacga gaagaauggc cugccggcua gagaagacag ggccauccgg     2640

guacacggac augagauaaa aucaagcgau uauauccagg ucuucucuaa gcguaaaaag     2700

acugacuccg aucgcgacga aacaccuuuc ggcgcaauug ccgugcgagg gggauuuguu     2760

gagaucggcc caaguauuca ccaugcuaga aucuacaggg uggaagguaa gaaacccguc     2820

uacgcaaugc uacggguguu cacccacgau cugcucagcc agcgccacgg ggaccuguuu     2880

ucugccguua uaccucccca guccauuuca augagaugcg cggagccaaa acuuaggaaa     2940

gcuaucacaa cuggaaacgc caccuauuug ggcugggugg uagucgguga ugagcuggaa     3000

auuaaugugg acaguuucac gaaguacgca aucggccggu uuuuagagga uuucccuaac     3060

accacacgau ggagaauaug uggguaugac acuaauagca aacucacccu gaagcccauc     3120

gugcuugccg cugaaggacu ggagaaccca ucguccgccg ucaaugaaau uguugaguug     3180

aaaggcuggc gcguggcaau caacguacuc acaaaggugc auccgacugu cgugaggcgg     3240

gaugcucugg ggcguccuag auacucuagc aggucaaauc uacccaccag uuggacgauu     3300

gaguga                                                                3306


<210>  2545
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2545
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

acuacggaua auuauaaagu uccuucuaag aaaaugaagg ugcucgguaa caccucaaag      120

aaguacauaa agaaaaaucu ucugggcgua uuguuauucg acagugggau cacagcugag      180

ggaagaaggc ugaagcggac ugcacgccga cguuauacca gaaggcggaa ccgcauucua      240

uaccuccagg aaaucuuuag cacagagaug gccacccugg augacgcguu cuuucaaaga      300

cuugaugacu ccuuccuggu gccagaugac aaaagggauu cuaaguaucc gauuuuuggc      360

aauuuggucg aagagaaggc uuaccacgac gaguucccca cuaucuauca ucuccggaag      420

uaccuggccg auucgacaaa gaaagcagac cugcgccucg uguacuuagc ccuggcucac      480

augauaaagu aucgagguca uuuucuuauc gagggggagu ucaacucaaa gaauaacgau      540

auucagaaga auuuucagga cuucuuggac accuacaacg caaucuuuga aagcgaucug      600

agucuagaaa auuccaaaca gcucgaagag auuguuaagg acaaaaucuc uaagcuggag      660

aagaaagaua gaauacuuaa gcuguucccu ggagaaaaga acagcggcau cuuuuccgag      720

uucuugaagc ucauuguggg caaucaagcc gacuucagga aaugcuuuaa ccuggaugaa      780

aaggcuucau uacacuucag uaaggagagc uaugacgaag aucuggagac gcuccuuggg      840

uacauuggag acgauuauuc ugacgucuuu cugaaagcca agaaauugua cgaugcgauc      900

cuacuguccg guuuccucac ugugaccgac aaugagacag aagcaccacu gucaagcgcc      960

augaucaagc gguauaacga gcauaaagaa gaccuugcuc uguugaagga guacauucgu     1020

aacauaaguc ucaagacuua caaugaagua uuuaaagaug acaccaagaa cggcuaugcc     1080

ggguacaucg auggaaaaac aaaucaggag gacuucuaug uuuaccugaa gaaauuacug     1140

gcagaguuug aaggcgcuga uuauuuccua gagaagauug accgcgaaga uuuucucaga     1200

aagcagagga ccuucgacaa cgguucuauc cccuaccaga uacaucugca agagaugcgg     1260

gccauucuug auaaacaggc gaaguuuuau ccuuucuugg ccaaaaauaa ggaacgaauc     1320

gagaaaaucc ugacguuucg cauuccauac uacguggggc cccucgcaag aggaaacucc     1380

gacuucgcuu ggagcaucag gaagcggaau gagaaaauua cuccuuggaa cuucgaagau     1440

gucauagaca aggagucguc ugccgaggcu uuuaucaauc gcaugacauc cuucgaucug     1500

uaucuuccag aagagaaggu gcugccgaaa cacucauugc ucuacgaaac cuuuaacgug     1560

uauaaugagc ugacuaaggu cagauucauu gccgaaagua ugagggacua ccaguuuuua     1620

gacagcaaac agaagaaaga uaucguucgu cuguauuuca aggacaagcg gaaagugaca     1680

gauaaggaca ucauugagua ccuacacgca auauauggcu acgauggcau cgaacucaaa     1740

gggauugaga agcaauuuaa cuccucucug agcaccuacc acgaccuuuu gaauaucauu     1800

aacgauaaag aguuccugga cgauucaagu aaugaagcca ucauagagga aaucauucau     1860

acgcucacca ucuuugagga ccgagaaaug auuaagcaga gacuguccaa guucgagaac     1920

aucuuugaua aaagcguauu aaagaaacug ucuaggcgcc acuauacagg augggguaaa     1980

cuuucagcua aauugauaaa cggcauucgg gacgagaaga gugggaauac uauccucgau     2040

uaccugauug acgacggaau cagcaacaga aauuucaugc agcuaauaca cgaugacgcg     2100

cuguccuuua agaaaaagau ccaaaaagca cagauuaucg gcgaugaaga caaggguaac     2160

auuaaagagg uggucaaguc ucuccccggg ucgccugcca ucaaaaaggg cauacugcag     2220

agcauuaaga ucguggauga acuuguuaaa gugaugggag gcaggaagcc agaguccauc     2280

gucguggaaa uggcucgcga gaaucaguau accaaccaag ggaaaucaaa uagucagcag     2340

cgauugaagc ggcuggagaa aucuuuaaag gaacucggaa gcaagauucu gaaagagaac     2400

auccccgcca aacuuuccaa aauugacaau aacgcacugc agaaugaucg uuuguaccuc     2460

uauuaccugc aaaacgguaa ggacauguau acaggcgaug accuagauau agacagacug     2520

ucaaauuacg acaucgauca uauuaucccu caggccuucc ucaaagacaa cagcauugau     2580

aauaagguac ugguuucuuc cgcuaguaac aggggaaaga gcgacgaugu gccaucucuu     2640

gaagucguga aaaagcgcaa aacuuucugg uaccaguugc ugaaguccaa auuaaucuca     2700

cagcggaagu uugacaaccu caccaaggcc gagagagggg gccugagucc cgaggauaaa     2760

gcagguuuca uacaaaggca gcuugucgaa acacgacaga ucaccaagca cguggcgcgc     2820

cugcuagacg agaaauuuaa uaacaagaaa gaugaaaaua accgggcugu uagaacugug     2880

aagauuauca cgcucaagag cacacuggua ucgcaguuca ggaaagacuu ugaguuguau     2940

aagguccgug aaauuaauga uuuccaucac gcccaugacg cauaccugaa cgccgugguu     3000

gcuuccgccu uacucaaaaa guauccgaaa cuggagccug aguuugugua cggggauuau     3060

cccaaguaca auucuuuccg cgaacggaaa agcgcaaccg agaaggucua uuuuuacuca     3120

aacaucauga auauauucaa gaaaaguauu ucccuugcug acggcagagu gaucgaaagg     3180

ccacugaucg agguaaacga agagacugga gagucugugu ggaauaagga aagcgacuug     3240

gccaccguuc gacggguccu cuccuacccu caagugaacg uggucaaaaa gguugaggaa     3300

cagaaucacg gccuggaucg cgggaaaccc aagggacuau uuaacgcgaa ucugucaagc     3360

aagccaaaac ccaacaguaa cgagaaucuu gugggugcca aggaauaucu cgacccuaaa     3420

aaguacggcg gguaugcugg aauuucuaac uccuucacag ugcugguaaa aggcaccaua     3480

gagaagggug caaagaaaaa gaucacuaau gucuuggagu uccaggggau uagcauccug     3540

gauagaauua acuacaggaa agacaaguua aauuuucucc uggaaaaagg cuauaaggau     3600

aucgagcuua uaaucgaacu gccaaaguac ucguuguucg agcucucaga cggaucucgg     3660

cgaaugcugg ccaguauucu auccacaaac aauaaaagag gcgagaucca caaggggaac     3720

caaauuuuuc ugagccagaa auucgugaaa cuucucuauc augcuaaacg caucucuaau     3780

acgauaaacg aaaaucacag gaaguacguu gagaaccaua aaaaggaauu ugaggaacug     3840

uucuacuaua uuuuggaguu uaaugagaac uacgugggag ccaagaaaaa uggcaaguua     3900

cugaacagcg cauuccaguc cuggcagaau cacucaaucg augaacucug uaguagcuuu     3960

aucgguccga ccgggucuga gcguaaagga cuguucgaac uuacuucccg gggcucagcg     4020

gccgacuuug aguuccuggg ugucaagauu cccagauaua gggauuacac accuaguagc     4080

uugcuaaaag acgcuacccu gauccaccaa uccgugacag gccucuauga aacucgcaua     4140

gaucuggcca aacuugggga gggauga                                         4167


<210>  2546
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2546
auggccgcuu ucaagcccaa cagcaucaau uacauucugg gccucgacau agggaucgca       60

uccgugggau gggccauggu cgagauugau gaagaggaaa acccuaucag acuuauugac      120

cuggguguua ggguguuuga gcgggcggaa guaccaaaaa ccggcgauuc uuuggcuaug      180

gcccgccgau uagcacguuc agugagaagg cugacacggc gcagagccca caggcuacuc      240

cggacucgcc gacugcuuaa gagagagggg guccugcagg cugcaaauuu cgacgagaac      300

ggauugauca aaagucuccc gaauacgccc uggcaacuga gggccgcugc ccuggaucgg      360

aagcucaccc cuuuagaaug gagcgcggug cugcuucauu ugauaaaaca ccguggcuau      420

cugucccagc gcaagaacga gggugaaaca gcagacaagg agcuaggggc ccuccugaaa      480

ggaguugcug gcaaugccca ugcacuucag acuggcgauu uuagaacccc agcugagcug      540

gccuugaaca aguucgaaaa agagucuggg cacaucagga aucagcgguc ggacuacuca      600

cauacauuua gccgaaagga ucuccaagcg gaacugauuu uacuguucga gaaacagaag      660

gaauuuggaa acccccacgu gagugguggc cucaaggagg ggaucgagac ccuucugaug      720

acucagcgcc cugccuuguc cggagacgca guccagaaaa ugcuaggcca cugcacauuc      780

gaaccagcug agcccaaggc cgcuaaaaau accuauacgg ccgaaagauu uauuuggcug      840

acuaagcuca acaaucugag gauccuugag caagguucug aacggccucu gaccgauaca      900

gagcgcgcaa cuuugaugga cgagccauac agaaaaagca agcucaccua ugcccaggcu      960

aggaaacugu uagggcugga agauacagcg uucuuuaagg gacuacguua cggcaaggac     1020

aacgcagagg ccuccacccu cauggaaaug aaggcuuauc augccauauc acgggcacug     1080

gagaaagaag gccuuaaaga caagaaaagu ccguugaauc ugagccccga gcuccaggau     1140

gagauuggga cggccuucuc ucuguuuaag acugacgaag auaucacagg acgacuuaag     1200

gacaggauuc agccugagau ccuggaggcu uugcucaaac acaucuccuu cgauaaguuc     1260

gugcaaauuu cacugaaagc cuuaagacgc auaguaccac ugauggaaca ggguaagcgg     1320

uacgacgagg caugugcgga aaucuacggc gaucauuaug ggaaaaagaa caccgaggaa     1380

aagauuuacc uacccccuau cccagcugac gagauaagaa accccguugu gcucagggcc     1440

cugagccagg cacgcaaagu cauuaaugga guggugcgac gguauggcag uccggcccgu     1500

auccacaucg aaacugcuag agaggucggu aagucuuuua aagauaggaa ggagauugaa     1560

aaacgccaag aggaaaaccg gaaggacaga gagaaggccg cagcuaaauu cagggaauac     1620

uuuccuaauu ucguugggga gcccaagucc aaagauaucc uuaaguugcg acuguaugag     1680

cagcagcacg gcaaaugccu cuacagcgga aaggaaauua accugggccg cuuaaaugag     1740

aaaggguaug uggaaauaga ccaugcccug ccauuuucgc ggacauggga cgauucuuuc     1800

aacaauaagg uacuuguguu gggauccgag aaccagaaua aggguaacca aaccccuuac     1860

gaauacuuua auggcaaaga caacucaaga gaguggcagg aguucaaggc gagggucgaa     1920

acgagucguu uuccccgcag caaaaagcag cggauccucc ugcagaaauu cgaugaggac     1980

ggguuuaagg aaagaaaucu aaacgauacc agguauguga aucgauuccu gugucaauuu     2040

guugccgacc ggaugcgccu cacaggaaag ggcaaaaaga gaguguucgc uuccaacggu     2100

cagauuacua accugcuuag gggguucugg ggcuugcgga aaguccgagc agagaaugau     2160

agacaccaug cccuggacgc ugugguaguu gccugcucua ccguggcaau gcagcagaag     2220

aucacacgcu uugucaggua caaagagaug aacgcguucg auggaaagac uaucgacaag     2280

gaaaccggcg agguguuaca ccagaaaaca cauuuuccac agcccuggga guucuuugcc     2340

caggaaguca ugauucgugu guucgggaag ccugauggaa aaccagaauu ugaggaggcu     2400

gacacccucg aaaagcugcg gacucuucug gccgagaaau ugagcucaag accggaagca     2460

guucacgagu augugacgcc ccucuucgua aguagggcuc cuaaucgcaa gauguccggu     2520

cagggccaca uggaaacagu caagagcgcc aaacgacugg augagggggu gucuguucua     2580

agagugccac ugacccaacu caagcugaaa gaccuugaga agauggucaa ccgggaaagg     2640

gagcccaaau uguacgaagc acugaaggcc cgcuuagagg cucauaaaga cgauccugcg     2700

aaggccuuug cagaaccauu cuauaaguac gacaaagcug gaaaucguac ucaacaggug     2760

aaggccguaa gaguggagca aguucagaaa accggcgucu gggugcggaa ccacaauggu     2820

auagccgaua acgcuacaau ggugaggguc gacguuuuug agaaggggga uaaauauuac     2880

cucgugccca ucuacucaug gcaaguggca aagggcauuc ugccggaccg cgccguaguc     2940

cagggaaagg augaagagga cuggcagcuu aucgaugaca guuucaauuu uaaauucagc     3000

cugcauccua acgaucuagu ggaaguuauu accaagaaag cgcgaauguu cggcuauuuu     3060

gcuuccuguc acagagggac uggaaauauc aacauacgga uccacgaccu cgaucauaag     3120

auuggcaaaa augguauccu ggaggggauu ggagugaaga cagccuuguc uuuccagaag     3180

uaccagaucg acgagcuggg caaagaaaua aggccaugcc gcuuaaagaa aagacccccu     3240

gucagguga                                                             3249


<210>  2547
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2547
augaagaaac ccuacaccau cggccuggac auugggacaa acagcguggg augggccguc       60

cucacugauc aguaugaccu uguuaagaga aaaaugaaga uagcugguga uuccgagaaa      120

aagcaaauca agaaaaauuu cuggggcgug aggcuguuug acgaagggca gacggcagcc      180

gaucggcgca uggcgcgaac cgcucguaga aggauugagc ggcgcagaaa caggaucucu      240

uacuugcagg gaauuuucgc cgaagagaug ucaaagacag acgcaaacuu uuucugccgg      300

uuaagugaua gcuuuuaugu agacaacgaa aaacgcaauu cccgacaccc uuucuuugcc      360

acuaucgagg aggaagugga guaccauaag aacuauccaa ccauauacca ccugagagag      420

gagcuaguca auucuucgga gaaagcugau cucaggcugg uguaucuugc acuggcccau      480

aucauuaagu accggggcaa cuucuugauc gaaggugcuc ucgacacaca gaauaccuca      540

guugauggga uuuacaagca auuuauccag acuuauaacc agguguucgc cagcggaaua      600

gaggacggca gucugaaaaa gcuggaagau aauaaagacg ucgcgaagau ucucguggag      660

aaaguaacac guaaggaaaa auuagagcgc auccugaaac uuuacccggg cgagaagucc      720

gcagggaugu uugcccaguu cauuucuuug aucguuggaa gcaaagguaa cuuucaaaag      780

cccuucgacc ugaucgaaaa auccgauauu gagugugcua aggacucaua ugaagaggau      840

cuagaaaguc uccuggcccu uauaggcgac gaguacgcag agcuguucgu ggcugccaaa      900

aaugcguaua gcgccgucgu guugucuucc aucauuaccg uggcagaaac ggagacuaac      960

gcuaagcucu cagccagcau gaucgaaaga uuugauaccc acgaggaaga ccugggggag     1020

uuaaaggcuu ucauaaaacu gcaucucccu aagcacuacg aggaaauuuu uaguaauaca     1080

gagaaacacg gauaugccgg cuacaucgau gguaagacua aacaggcaga cuucuacaag     1140

uauaugaaga ugacccuuga aaacaucgag ggggccgauu acuuuauugc uaaaaucgag     1200

aaggaaaacu uccugaggaa acagcggaca uuugacaaug gagcgauucc acaucaguug     1260

caccuagagg aacuggaggc aauacuccau caacaggcca aguauuaccc cuuccugaaa     1320

gaaaacuaug auaagaucaa gucucuuguc accuuucgca uuccuuacuu cguuggccca     1380

cuggcuaaug gccaguccga auuugccugg uugacgcgaa aggcagacgg ggagaucaga     1440

cccuggaaua ucgaagagaa gguggacuuc ggaaaaagcg ccguagauuu uauugaaaag     1500

augacuaaua aagacacaua ucucccuaag gagaacgugc ugccaaaaca cucguuaugc     1560

uaccaaaagu accuggucua uaaugaacua accaagguga gguacauaaa cgaucagggu     1620

aaaacuucuu auuucuccgg ccaggagaag gagcagaucu ucaaugaccu cuuuaaacaa     1680

aagcggaaag uuaagaagaa agaucuggaa cuuuucuugc gcaacauguc acacguggag     1740

aguccgacaa uugaagggcu ggaggacagc uuuaauuccu cuuacagcac cuaucaugau     1800

cuccugaagg ucggaaucaa acaggaaauu cuugacaacc ccgugaauac ggagaugcug     1860

gagaacaucg uaaagauauu gaccguuuuc gaagauaaaa gaaugaucaa ggagcagcuc     1920

cagcaauuuu cagacgugcu ggauggcguc guguuaaaga aacuggaaag gcgucacuac     1980

acagguuggg ggcggcuaag ugcuaagcuc cugaugggca uucgagacaa acagucccau     2040

cuuacuaucu uggauuaucu gaugaaugac gacggacuca acagaaaccu gaugcaguua     2100

auuaaugaua gcaaccuguc uuucaaguca aucauagaga aagagcaggu caccacagcc     2160

gacaaggaua uucaaaguau cguggcagac cuugcgggca gcccugcuau uaagaaaggg     2220

aucuugcagu cccucaagau aguugaugaa cuggugucug uaaugggaua cccaccccag     2280

acuaucgucg uggagauggc cagggaaaau cagaccacag guaaaggcaa gaacaauucg     2340

cgcccucggu acaaaagccu agagaaggca auuaaagaau uuggguccca aauccugaag     2400

gagcacccaa ccgacaacca ggagcucaga aauaacaggc uguaucuuua cuauuugcag     2460

aauggaaagg auauguacac uggccaagac cuggauauuc auaacuuauc aaauuaugac     2520

aucgaucaca uaguucccca gaguuucauu acggacaacu cuaucgauaa ucucgugcug     2580

acaagcuccg ccgguaaccg cgaaaaaggg gacgacgucc cgccucuuga gaucgugcga     2640

aagcggaaag uauuuuggga aaagcuguac cagggcaauu ugaugucaaa acguaaguuc     2700

gauuaucuca ccaaggcuga gagaggaggc cugacugaag ccgacaaagc aagguuuauu     2760

caccgccagc uaguggagac ccggcaaauc acaaagaacg uugcuaacau ucugcaucag     2820

agauucaauu acgagaaaga ugaccacggg aacaccauga agcaggucag gauagugacu     2880

cucaaaagcg cccugguguc ucaguuucga aagcaauucc agcuuuacaa gguccgcgau     2940

guuaaugacu aucaucacgc gcacgaugcc uacuugaacg gagugguggc uaauacacug     3000

uuaaaaguau auccccagcu cgaaccagag uucgucuacg gugacuauca ucaguuugau     3060

ugguucaagg caaacaaagc cacggcuaag aaacaauuuu acaccaauau caugcuguuc     3120

uuugcccaga aggaccggau uaucgaugaa aacggcgaga uucuuuggga caagaaauau     3180

cuggacacug ugaagaaagu uauguccuac agacagauga auaucgugaa gaaaacagaa     3240

auacaaaagg gggaguucag uaaggcaacc aucaaaccua agggaaacag cucuaaacua     3300

auucccagga agacaaauug ggauccaaug aaauacggcg gucucgacuc ccccaacaug     3360

gcguaugccg ucgugaucga guacgcuaag gggaaaaaua agcugguuuu ugaaaagaaa     3420

auuauccgug ugacuauaau ggagcgcaag gccuucgaaa aagaugagaa ggcauuuuug     3480

gaggaacagg gcuaucggca gccuaaagua cuggcuaagu uaccaaagua cacccucuau     3540

gagugugaag agggaagaag gcgaaugcug gccucagcaa acgaagccca gaaaggcaau     3600

caacaggucc uuccgaacca ccuggugacg uugcuccauc acgcugcgaa cugcgaggug     3660

agugacggaa agagccugga uuacauugag ucgaaucggg aaauguucgc cgagcuacug     3720

gcacaugucu ccgaauuugc uaaacgcuau acccuugccg aggccaaccu caauaagauc     3780

aaccagcugu ucgaacagaa uaaagagggg gacaucaagg cuauugcaca aucuuucguu     3840

gauuugaugg ccuuuaacgc gaugggcgcu cccgccagcu ucaaguuuuu cgagacaacu     3900

auagaaagaa aaagguacaa uaaccugaag gaguuacuca auucaaccau cauuuaccag     3960

aguaucacag gucuguauga aucccggaaa cgacuugacg auuga                     4005


<210>  2548
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2548
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

guuacagaug acuauaaggu gccugcuaaa aagaugaaag uacucgguaa uacugauaag      120

ucucacauag agaagaaccu ucugggcgca uuguuauucg acucagggaa uacggccgaa      180

gauagaaggc ugaaacggac cgcgcgccga cguuacacaa gaaggcggaa ccgcauccua      240

uaucuccagg agauuuuuag ugaagagaug ggaaaggugg acgauagcuu cuuucauaga      300

cuggaagacu ccuuccuugu cacugaggau aaaaggggcg agcggcaccc aaucuuuggu      360

aaucuggaag aggaagugaa guaccaugag aacuucccga ccauuuauca cuugcgccaa      420

uaccucgcug acaaucccga gaaaguugau cugcgacugg uguaucucgc cuuagcacau      480

aucauaaagu uuagagggca cuuccugauc gaaggaaagu uugacacaag gaacaaugau      540

guccagcggc uuuuccagga guuuuuggcc guguacgaca acaccuucga aaauucuucg      600

cugcaggagc aaaacguaca gguugaagag auucuaacug acaaaaucuc aaagagcgcu      660

aaaaaggauc gugugcucaa acuguuuccu aaugagaaga guaacggccg cuucgcagag      720

uuccuuaaac ugauugucgg caaucaggcc gacuuuaaga agcacuucga guuggaagag      780

aaagcuccac uccaguuuuc caaggauaca uacgaagagg agcuggaagu guuacuggcc      840

caaaucgggg acaacuaugc ggaacucuuc cuuucugcca aaaagcugua cgauagcaua      900

uugcuauccg guauucugac cgugacggac gucggaacua aagcaccccu cucagcuagu      960

augauccaga gauauaacga acaucagaug gaucuggccc agcuuaagca auuuauuagg     1020

cagaagcuga gcgacaaaua caaugagguu uucucugaug uguccaagga cggcuaugca     1080

ggguacaucg auggaaaaac caaccaggaa gcauuuuaua aguacuugaa aggccuccug     1140

aauaagaucg aggguucagg guacuucuua gacaagauug agcgggaaga cuuucugcga     1200

aaacaacgca cauucgauaa cggaagcaua ccucaccaga uccaucuaca ggagaugaga     1260

gccauuauca ggcggcaggc ggaauuuuau ccauuccucg ccgacaauca agaucgcaua     1320

gagaagcugc uuacuuuuag gauucccuac uauguaggcc cuuuggcaag aggcaaaagu     1380

gacuucgcuu ggcugucucg uaaguccgcc gauaaaauca ccccauggaa cuucgacgag     1440

aucguggaua aggaaagcuc ggcugaggcc uuuauuaauc ggaugacaaa cuacgaccuc     1500

uaucugccga aucagaaggu ccuucccaaa cacucucugu uguacgaaaa guucaccgug     1560

uauaacgagc ucacgaaagu uaaguacaaa acugaacagg ggaagacagc auuuuucgau     1620

gccaauauga aacaggagau cuuugacgga guguucaagg ucuaccgaaa ggugaccaaa     1680

gauaagcuga uggacuuuuu agagaaagag uucgaugaau uuagaauugu agaccugacu     1740

ggucuagaca aggaaaacaa aguuuucaau gcuuccuaug gcacauacca cgaucucugc     1800

aagauacugg acaaggauuu ucuugacaac aguaaaaaug agaagaucuu ggaagauauu     1860

gugcugaccc ucacgcuguu cgaggacagg gagaugaucc gcaaacgguu agaaaacuau     1920

ucagaucugc uuaccaagga gcaagucaaa aaguuggaaa gaaggcauua cacagggugg     1980

ggacgccuca gcgcggagcu gauccacggc auucgaaaca aggaaucccg gaaaacuaua     2040

cuagacuauc ugaucgauga cgguaauucu aaccguaauu uuaugcagcu cauuaacgau     2100

gacgcacuga gcuucaagga ggagaucgcc aaagcucagg ugauugggga aaccgauaau     2160

cuuaaccagg ucgugucaga caucgccggc aguccugcaa uaaagaaagg aaucuugcaa     2220

ucccugaaga uuguugacga guuagugaag aucaugggcc aucagccaga aaauauugua     2280

gucgagaugg ccagagagaa ccaguucaca aaucagggga ggcgcaacag ccaacagcgg     2340

cucaaaggac ugacugauuc uaucaaggaa uuugguucac agauacuuaa agagcacccc     2400

guggaaaaua gucaacugca gaacgacaga uuguuccucu acuaucugca gaauggcagg     2460

gauauguaca ccggggagga acuagacauu gauuaccuga gccaguauga caucgaucau     2520

auuaucccuc aggcuuuuau aaaggacaac uccaucgaua aucgaguucu cacaucuucg     2580

aaagagaacc gcggaaagag cgacgaugug ccauccaaag acgucgugcg gaagaugaag     2640

ucauacugga guaaacugcu uucugccaag uugauuaccc aaagaaaauu cgacaaccug     2700

acuaaggcag agaggggcgg uuuaacggau gacgauaaag cgggguuuau caagcgucag     2760

cucguagaaa cacgccagau uaccaagcac guggcucgga uccuggacga gagauucaau     2820

acugaaaccg augagaacaa uaaaaagaua aggcaaguua aaauugucac acuuaagagc     2880

aaccuggugu ccaauuuucg aaaagaguuc gaacuauaua aggugcggga gaucaacgac     2940

uaccaccaug cccacgaugc auaucucaau gccgucaucg gcaaggcucu guugggaguu     3000

uacccccagc uggaaccgga guuuguguau ggcgacuacc cucauuucca cgggcacaaa     3060

gaaaacaagg ccaccgcuaa aaaguuuuuc uacucaaaua uuaugaacuu cuuuaaaaag     3120

gaugacgugc gcacugauaa gaauggagag aucauuugga aaaaggacga acauauaagc     3180

aacaucaaaa agguauuauc uuauccccag gucaauauug ugaaaaaggu ugaggagcag     3240

acagguggcu ucuccaagga aaguauccuc ccaaaaggga acagcgauaa gcugauuccu     3300

agaaaaacga agaaauuuua cugggacacc aagaaauaug gaggcuucga cucucccauc     3360

guggcuuacu ccauacuugu caucgccgau auugagaagg guaagaguaa aaagcugaaa     3420

acugugaagg cguugguugg ggugacaauc auggagaaaa ugaccuuuga aagggaccca     3480

guagccuucc ucgagcggaa gggcuaucga aauguccaag aagagaacau uaucaagcug     3540

cccaaauacu cacuauuuaa gcuggaaaac ggaagaaaac gccuucucgc uagcgcaagg     3600

gagcugcaga agggcaauga aauaguguug ccuaaccacc uggggacauu acucuaucau     3660

gccaaaaaua uucacaaggu ggaugagcca aagcaucugg acuacgucga uaaacacaag     3720

gacgaguuca aagaacuucu ggauguugug ucgaacuuuu ccaagaaaua cacuuuggcu     3780

gagggaaauc ucgaaaagau caaggagcug uaugcccaga acaauggcga agaccuaaaa     3840

gagcuggcau cuagcuucau caaccuucuc accuuuacgg cgauuggugc cccggcuacc     3900

uucaaguuuu ucgauaaaaa uauagaccgu aagcgguaca caucaacuac cgagauccug     3960

aacgccacau ugauucacca aaguaucacu ggguuauaug aaaccagaau ugaucugaau     4020

aaacucggag gcgacuga                                                   4038


<210>  2549
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2549
augcagacca caaaccugag cuacauccuc ggccuugacc uggggauugc cuccguggga       60

ugggcugucg uugagauaaa ugaaaacgag gaucccaucg guuugauuga cgugggcgua      120

agaaucuucg aaagggcaga ggugccuaag acuggggaau cuuuagcccu gucacggcgc      180

cuagcgcgaa guacgcguag acucauuagg cggcgcgcuc acagacugcu ucuggccaaa      240

agguuuuuga agcgggaggg aauccucagc accauagauc uggagaaagg ccugccaaau      300

caagcauggg aacuccgcgu cgccgguuua gagcgaagac uguccgcuau cgaaugggga      360

gcagugcuuu ugcaucugau uaagcacagg ggguaucuau cuaaacggaa gaacgagucg      420

cagacaaaua acaaggagcu cggcgcccug cuuucaggcg uugcucagaa ucaucagcug      480

uugcaaagcg acgauuaccg uacuccggcc gaacucgcgc ugaaaaaguu cgcaaaagag      540

gaagggcaca uccgcaacca gagaggagcc uauacccaua cauuuaauag guuagaccug      600

cucgcugagc uuaaccuguu guucgcccag cagcaccaau uugguaaucc ccacugcaag      660

gaacauauuc agcaguacau gaccgagcua cugauguggc agaaaccugc acucaguggc      720

gaggcuaucc ugaagaugcu ugggaagugu acucacgaaa agaacgaguu caaggccgcg      780

aaacauacau auuccgccga acgguuugug uggcugacca aguugaauaa ccuccgaaua      840

cuggaggaug gagcagaacg cgcuuuaaau gaggaggaaa gacaacugcu aauuaaccac      900

ccauacgaga aaucuaagcu cacguaugcc caggucagga aacugcuugg cuugagcgaa      960

caggcuaucu ucaagcaccu ccgguacucc aaggagaacg ccgaaucagc aacuuuuaug     1020

gagcucaaag ccuggcaugc uauucgcaag gcgcuggaga aucaaggucu uaaagacacc     1080

uggcaggauc uggcaaagaa acccgacuug cucgaugaaa ucgggacagc cuucagucug     1140

uacaagacug acgaggauau ccagcaguau uuaaccaaca aggugccuaa uagcguaauu     1200

aacgcucugc uaguuucucu caauuuugac aaauucauag aacugucccu uaagucauug     1260

agaaaaaucc ugccacucau ggagcaagga aagagguacg accaggccug ccgugagauu     1320

uauggccacc auuacggcga agcaaaccag aaaacaagcc agcuguuacc cgccaucccu     1380

gcucaagaga uacggaaucc agugguccug cgaacccuua gucaggccag aaaggugauu     1440

aacgcaauca ucaggcagua ugggucuccg gcgcgcgugc acauugaaac gggacgggag     1500

uuggguaagu ccuuuaaaga aagaagggag auccagaagc aacaggagga uaaucgcacu     1560

aaacgagaaa gcgcugucca gaaguucaaa gagcuguucu cggacuuuuc uuccgaaccc     1620

aagucaaagg auauucugaa auuccggcua uacgagcagc aacauggcaa gugucuguau     1680

agugggaaag aaauaaacau ccaccgucuc aaugagaagg gauacguuga gauugaccac     1740

gcccugccuu uuagcagaac augggaugac uccuucaaca auaaagugcu uguauuggca     1800

ucugaaaacc agaauaaggg caaccagacc ccauacgagu ggcugcaagg uaaaaucaac     1860

agcgaaaggu ggaagaauuu uguggccuua guccucgggu cacagugcag ugcugccaag     1920

aaacagcgcc ugcuuacuca ggugaucgau gacaacaagu ucauugaucg gaaucugaac     1980

gacacaagau auauagcaag guuuuugucc aauuacaucc aagagaaccu ccugcuaguu     2040

ggcaaaaaua agaaaaacgu guucaccccc aauggacaga uuacggcucu gcuccgaagc     2100

cgcuggggcc ugaucaaggc ccgggaaaac aauaacagac aucacgcgcu ugaugccauu     2160

gucguggcuu gugcaacccc uucuaugcag cagaagauca caagguuuau acguuucaaa     2220

gagguacauc cauauaagau cgagaaucgc uacgaaaugg uugaccaaga gucaggggaa     2280

auuaucaguc cccacuuucc ggagccuugg gccuauuucc ggcaggaggu gaacauuaga     2340

gucuuugaca aucaccccga uacuguguug aaagaaaugc ugccagacag gccucaggcu     2400

aaccaucagu ucguccaacc cuuauucgug agccgagccc caacccggaa gauguccgga     2460

cagggucaca uggagacaau caaaucugca aagcgccucg cggaaggcau aucgguucug     2520

aggauucccc uuacucagcu gaagccuaac cuacucgaga auauggugaa caaagaaaga     2580

gagccagccc uguacgcugg guugaaggcc cggcuggcag aguuuaauca agauccggcu     2640

aaagccuucg caacccccuu uuauaagcag ggaggccagc agguaaaagc cauccgaguc     2700

gaacaagugc agaaaagcgg uguuuuagug agagagaaca auggggucgc ugacaacgcg     2760

uccauugugc gcacagaugu auucaucaag aauaacaaau uuuuccucgu gccuauauac     2820

accuggcagg uugccaaggg cauccugcca aauaaagcaa uugucgcuca uaagaacgaa     2880

gacgaguggg aagagaugga ugagggagcc aaauuuaagu ucucacuuuu ucccaaugac     2940

cugguggaau ugaaaacuaa gaaggaguac uucuuuggcu auuacaucgg gcucgauagg     3000

gccacgggaa acauuagucu gaaagaacac gacggugaga ucucuaaggg caaagauggg     3060

guguaucgug ucggaguuaa gcuagcucug agcuucgaaa aauaccaggu ggacgagcuu     3120

ggcaagaauc ggcaaauaug cagaccucag cagaggcagc cagugcgcug a              3171


<210>  2550
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2550
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagacgaga acccaaucug ccuuauugau      120

cuggguguua gaguguuuga aagggcggag guaccgaaaa ccggcgacuc cuuggcuaug      180

gcccggcgcu uagcacgauc ugugcguaga cugacaaggc ggcgcgccca cagacuacuc      240

agggcucggc gccugcuuaa gcgagaaggg guccugcagg cagccgauuu cgacgagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gagcugccgc gcuggauagg      360

aagcucacgc cauuagagug gagugcagug cugcuucauu ugauaaaaca ccggggcuau      420

cugagccagc guaagaauga aggugagacc gccgacaagg aacuaggggc ucuccugaaa      480

ggaguugccg auaacgcaca ugcccuucag acaggcgacu uucgcacucc cgcugagcug      540

gcguugaaua aguucgagaa agaauccggc cacaucagaa accagcgagg ggauuacucu      600

cauaccuuuu cgcggaagga ccuccaagcc gagcugauuu uacuguucga aaaacagaag      660

gaguuuggaa auccucacgu gucagguggc cucaaggaag ggaucgagac acuucugaug      720

acccagcgac cagcauugag cggagaugcu guccagaaaa ugcuaggcca cuguacuuuc      780

gagcccgccg aaccuaaggc ugccaaaaac acauauaccg cagagcgcuu uauuuggcug      840

acgaagcuca auaaccugag aauccuugaa caagguagug agaggccacu gacugacacc      900

gaacgggcca cauugaugga cgagccguac cgcaaaucca agcucacuua ugcucaggcg      960

agaaaacugu uagggcugga ggauaccgca uucuuuaagg gacuaaggua cggcaaggac     1020

aaugccgagg cuucuacacu cauggaaaug aaagccuauc augcaauaag ccgugcccug     1080

gaaaaggagg gccuuaaaga uaagaaaucc cccuugaacc ugucaccuga acuccaggac     1140

gagauuggga ccgcuuucag ucuguuuaag acggaugagg acaucacugg acggcuuaag     1200

gauaggauuc agccagaaau ccuggaggcc uugcucaaac acaucagcuu cgacaaguuc     1260

gugcaaauuu cucugaaagc auuaagaagg auaguacccc ugauggaaca ggguaagcgc     1320

uacgaugagg cgugcgcuga gaucuacggc gaccauuaug ggaaaaagaa uacagaagag     1380

aagauuuacc uaccuccaau ccccgccgau gaaauacgga acccgguugu gcucagagca     1440

cugucccagg ccaggaaagu cauuaacgga guggugcgcc gauauggcuc acccgcucgg     1500

auccacaucg agaccgcccg ugaagucggu aagagcuuua aagacagaaa ggagauugag     1560

aaaaggcaag aagagaaucg caaggaccgg gaaaaggcag cugccaaauu cagagaguac     1620

uuucccaacu ucguugggga accaaagagu aaagauaucc uuaaguugag gcuguaugag     1680

cagcagcacg gcaaaugucu cuacucugga aaggagauua aucugggccg auuaaacgaa     1740

aaaggguaug uggagauaga ccaugcgcug ccuuuuuccc gcacuuggga ugacagcuuc     1800

aauaacaagg uacuuguguu gggaucggaa aaucagaaca aggguaauca aacacccuac     1860

gaguacuuua acggcaaaga uaauucucgg gaauggcagg aguucaaggc cagagucgag     1920

accuccaggu uuccacguag uaaaaagcag cgcauccucc ugcagaaauu cgacgaagau     1980

ggguuuaagg agcggaaccu aaaugacacg agauauguga acagguuccu gugccaauuu     2040

guugcugauc gaaugcggcu caccggaaag ggcaaaaagc gcguguucgc aucaaauggu     2100

cagauuacaa accugcuuag aggguucugg ggcuugagga aaguccgggc cgaaaacgac     2160

cgacaccaug cucuggaugc cgugguaguu gcauguagca cuguggcgau gcagcagaag     2220

aucaccagau uuguccgcua caaagagaug aaugccuucg acggaaagac aaucgauaag     2280

gagacuggcg aaguguuaca ccagaaaacc cauuuucccc agccuuggga guucuuugcu     2340

caggaaguca ugauuagggu guucgggaag ccagacggaa aaccggaguu ugaagaggcc     2400

gacacacccg agaagcuccg uacccugcuu gcagaaaaac uguccucucg gccugaggcu     2460

guucacgaau augugacucc auuguucgua agcagagccc ccaacaggaa gaugucaggu     2520

cagggccaca uggagacggu caagagugca aaacgccucg augaaggggu guccguucug     2580

cgagugccuc uaacacaacu gaagcucaaa gaccuggaga agauggucaa uagagagcgg     2640

gaaccaaaac uuuacgaggc cuugaaggcu aggcuggaag cgcauaaaga ugaccccgcc     2700

aaggcauuug cugagccguu cuauaaguac gauaaagccg gaaaccgcac ccaacaggug     2760

aaggccguac guguggaaca aguucagaaa acuggcgucu gggugagaaa ucacaacggu     2820

auagcugaca augcaaccau ggugcggguc gauguuuuug agaaggggga caaauauuac     2880

uuagugccua ucuacagcug gcaaguggcc aagggcauuc ucccagauag ggcgguaguc     2940

cagggaaagg acgaggaaga uuggcagcug aucgacgauu cuuucaacuu uaaauucuca     3000

cuucauccca augaccuggu ggagguuauu acaaagaaag cucgcauguu cggcuauuuu     3060

gccaguugcc accgagggac cggaaacauc aauauaagaa uccacgaccu agaucauaag     3120

auuggcaaaa acgguauccu cgaagggauu ggagugaaga cugcacugag cuuccagaag     3180

uaccagaucg acgaguuggg caaagagaua cggccuugua ggcugaagaa acgcccucca     3240

gucagauga                                                             3249


<210>  2551
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2551
augaaguacc acgugggcau cgacgucggg accuucagcg uuggacuggc cgcuauugag       60

guggaugacg cagguaugcc cauaaaaaca cucucccuug uaucucauau ccacgauuca      120

ggccuggacc cugaugaaau uaagagugcc gugacuagau uggcgagcuc cgggaucgcu      180

aggcggacgc gccgauuaua ucguagaaaa aggcggcgcc ugcagcaacu agacaaguuu      240

auucagagac agggauggcc agucaucgag cucgaagauu acucugaccc gcuguauccc      300

uggaaaguga gggccgagcu ugcagccucg uacauagcug augaaaagga gcggggcgag      360

aagcugucag uugcauugcg ccauaucgcc cgacacagag guuggaggaa cccuuaugcu      420

aaagugagca gucucuaccu gccagacggg cccuccgaug ccuucaaggc gauucgggaa      480

gagaucaaac gugcaucugg acagccuguc ccagaaaccg ccacaguggg ccaaauggua      540

acucugugcg agcucggcac cuuaaagcug cgcggggagg gagguguucu uagcgcuaga      600

uugcagcagu ccgacuaugc cagggaaauu caggagaucu gucggaugca agaaauaggc      660

caggagcugu accgaaaaau uaucgaugug gucuuugcag cugaaucacc caaggggagu      720

gccagcucuc gcgugggaaa ggacccucua cagccaggca aaaauagagc gcucaaggcc      780

uccgacgcau uccagaggua ccggauugcu gcccugaucg guaaccuucg cgugagaguc      840

gauggggaga aaaggauccu gucaguugag gaaaagaauu ugguguuuga ccaucucgua      900

aaccugacac cgaaaaagga gcccgaaugg gugaccauug cugagauauu aggaaucgau      960

cguggccaac ugauuggcac ugccacaaug accgacgaug gggaacgggc aggagcccga     1020

ccuccaacgc acgacacuaa uagaagcauc gucaacagua ggauagcucc ccucguggau     1080

ugguggaaaa ccgcgucugc acuugagcag cacgccaugg uuaaggcucu guccaaugcc     1140

gagguggacg auuucgacag cccugaaggu gcaaaggucc aggccuuuuu cgcugauuug     1200

gacgacgaug ugcaugccaa acuagacucg cugcaccucc caguaggccg cgcagcguau     1260

ucugaggaua cacugguucg gcuuacuaga aggaugcugu ccgacggggu ggauuuguac     1320

accgcucgcc uccaagaauu uggaauugag cccucaugga caccgccuac gccccgaauc     1380

ggcgaaccag ucgguaaccc ugccguggac cggguccuga agaccgugag ucguugguua     1440

gagagcgcaa cuaaaacaug gggagccccc gagagaguua ucauugaaca ugugagggag     1500

ggcuucguaa ccgaaaagcg cgcucgggag auggauggag acaugagaag gcgagccgca     1560

cgcaaugcua aacuguuuca ggagaugcag gaaaagcuaa acguccaggg caagccaucc     1620

cgggccgauc ucuggagaua ucaaucugug cagaggcaga auugccagug ugcguacugc     1680

gggagcccca ucacuuucuc aaacagugag auggaccaca uuguuccucg ugccggacaa     1740

gguuccacaa auacccgcga aaaccuggug gcugucuguc accggugcaa ucagagcaaa     1800

ggcaacacgc cauuugcaau augggccaag aacaccucua ucgagggggu gucaguaaaa     1860

gaagcugugg agagaacaag gcauuggguu acugauaccg gaaugcgaag uacagacuuc     1920

aagaaauuca cuaaggccgu cguggagcgg uuucagcgcg caaccaugga ugaagagauu     1980

gacgcgagaa gcauggaauc cguggccugg auggcuaaug agcuuagguc ucgggucgcc     2040

cagcacuucg caucgcaugg cacaaccguu cgaguguaua gagguagcuu gacugcugaa     2100

gcccgcaggg cauccgggau cucaggcaag cugaaauuuu ucgacggagu gggcaagagu     2160

cgucucgauc ggagacacca ugccaucgac gcugcgguaa uugccuuuac gucugauuac     2220

gucgcagaga cacuggcugu gaggagcaac cuuaaacaau cccaggccca ccgccaggag     2280

gccccgcagu ggcgagaguu caccgggaag gacgcugaac acagagcagc cuggcggguu     2340

uggugucaga aaauggaaaa gcugucagcg uugcucacug aggaucugag ggacgaucgc     2400

guggucguga ugagcaaugu ucguuuaaga cugggaaacg guucugcuca uaaggaaacc     2460

auaggcaaac uauccaaggu gaaacucagu agccaacugu cuguauccga caucgauaag     2520

gccucaagug aggcacuuug gugcgccuug acacgggagc ccggguuuga cccuaaagaa     2580

ggacugccag cuaaucccga gaggcacauu cgcgucaacg gcacccaugu guaugcaggg     2640

gauaauaucg gucucuuccc ugugagcgcc ggcucgauug cgcugcgagg aggcuacgcu     2700

gaauuagggu ccucuuuuca ccacgccaga gucuacaaga ucacuagcgg gaaaaagcca     2760

gcauucgcca ugcugcgggu uuauacaaua gaccuuuugc ccuacaggaa ccaggaucuc     2820

uuuucagugg agcugaagcc gcaaacgaug aguaugcgcc aggcugagaa aaagcuaaga     2880

gacgcccugg caaccggcaa ugcugaauau cucgguuggc ugguagugga cgaugagcuu     2940

gucguggaca cuuccaaaau cgccacagau cagguuaagg cgguggaagc cgaguugggg     3000

accauuaggc gguggcgugu cgacggauuc uuuucuccua gcaaacugcg cuuacgacca     3060

cuccagaugu ccaaggaagg caucaagaaa gagucagcac ccgagcugag caagauuauc     3120

gauagaccug guuggcuucc cgcugugaac aaacuguuca gugacggcaa uguuacagug     3180

gucaggcggg auucuuuggg gcgcguaaga cucgaaucca cugcccaucu gccagugacc     3240

uggaagguuc aauga                                                      3255


<210>  2552
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2552
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagucgagaa ucccaagaca      120

ggugaaucac uugcccugcc ucggcgcuug gcgcgaagug cucguaaaag auuagccagg      180

cggaaggcac gccugaacca ccuaaaacau cucaucgcca augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcuaaggcau acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcccucaau gaacugcugu caaagcaaga uuucgcucgg      360

guuauccucc acauagccaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag cgauuuuaaa ggcaaucaaa cagaaugaag agaagcuggc caacuaucag      480

agugugggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg uaagaaauaa aaaggagucu uacgaaaggu guauugcuca gagcuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa caacgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaagugcu cagugucgcc uuuuauaagc gugcacugaa ggauuucagc      720

caucuugugg ggaacugcuc uuucuuuacg gacgagaaac gcgcuccgaa gaauaguccc      780

cuggccuuua uguucguugc guugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa ucuuauacac uaaggaugac cucaaugccc uucugaacga gguguugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucaga cgauuacgag      960

uuuaaggggg aaaaaggaac uuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcacuuggug agcacaaccu gagccaggac gauuugaaug aaaucgcuaa agacauuaca     1080

cucauaaagg augagaucaa acugaagaaa gccuuagcua aguaugaccu gaaccaaaau     1140

cagauugaua gucuaucuaa acucgaguuu aaggaccauc ugaacaucuc cuucaaggcc     1200

cuuaaauugg ucaccccucu gaugcucgaa ggcaagaaau acgaugaggc auguaaugaa     1260

cugaaccuua agguggccau aaaugaggac aaaaaggauu uucugccagc uuucaacgag     1320

acguacuaua aggacgaagu aacuaauccc guuguguuga gggcgauuaa agaguaccgg     1380

aagguccuca acgcacugcu gaaaaaguau gggaaagugc acaagaucaa uaucgaauua     1440

gcccgagagg ugggaaagaa ccacagccag cgcgcuaaaa uugaaaagga gcagaacgag     1500

aauuacaaag ccaagaaaga cgcagaacua gagugcgaaa agcucggccu gaagaucaac     1560

ucgaaaaaua uucuuaaguu gagacuguuc aaagagcaaa aggaauuuug ugccuauucu     1620

ggugagaaaa uaaagaucuc cgaucuccag gacgagaaaa ugcuggaaau ugaucauauc     1680

uacccuuauu caaggaguuu cgacgauagc uacaugaaca aggucuuagu uuuuaccaag     1740

cagaaucaag agaaacugaa ccagacacca uucgaggcuu uugggaauga cuccgccaag     1800

uggcagaaaa ucgaagugcu ugcaaagaac uugcccacua aaaagcagaa gcggauucuc     1860

gauaaaaauu acaaggacaa agaacaaaag aacuucaaag aucgcaaucu gaacgacacc     1920

agauauauag cgaggcuagu acugaauuac acaaaggauu aucucgacuu ucugccucuu     1980

ucugaugacg agaacaccaa guugaaugac acgcagaaag gaagcaaggu gcacgucgag     2040

gcuaaaucag gcaugcugac uuccgccuua cgucauacau ggggcuucuc cgcaaaggau     2100

cggaacaacc accuccacca ugccaucgac gcugugauua ucgccuacgc aaauaacagc     2160

auuguuaagg cuuuuucuga uuucaaaaag gaacaggagu caaauagugc cgaacuguau     2220

gcgaaaaaga ucagcgagcu ugacuacaaa aacaagcgaa aauuuuucga gccauuuucc     2280

ggguucagac agaaggugcu ggauaagaua gacgaaaucu ucgucucuaa accggagagg     2340

aagaaacccu cgggagccuu gcacgaagag accuuucgca aggaagagga guucuaucaa     2400

agcuacggug gcaaagaagg ggugcucaag gcucuggagc uaggaaaaau ucggaaggua     2460

aauggcaaga ucguuaaaaa cggugauaug uuuagagugg acauuuucaa gcauaaaaag     2520

acuaauaaau uuuacgcagu cccuaucuau acaauggauu ucgcccugaa ggugcuccca     2580

aacaaggcug ucgccagguc caaaaagggg gaaauaaaag acuggauucu gauggaugag     2640

aauuacgaau uuugcuucuc acuuuauaag gacaguuuga uccugauuca gaccaaagau     2700

augcaggagc ccgaguuugu guacuauaac gcauucacgu cuagcaccgu uuccuuaauc     2760

gugucaaagc acgacaauaa guuugaaaca cucagcaaaa accagaagau acuguucaaa     2820

aaugcgaacg agaaggaagu aaucgccaaa ucuauuggca uccaaaaucu uaaggucuuc     2880

gagaaguaca uuguguccgc ucugggagaa guuacuaaag ccgaguuucg ccagcgagag     2940

gacuucaaga aauga                                                      2955


<210>  2553
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2553
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu ugguaaggcg gacaaaccgc caaggacgac guuuagccag aaggaagaaa      180

caucggcgcg ugagacugaa uaggcuauuu gaggaaucag gccucauuac ugacuucacg      240

aagaucagua uaaaccugaa uccuuaccag cuucggguca aaggucugac cgaugaguug      300

agcaacgaag aacucuuuau cgcgcugaag aauaugguga agcaccgcgg gauuuccuau      360

cuggacgaug cuucugacga uggaaacucg ucaguuggcg acuacgccca gaucgugaaa      420

gagaauagca agcagcucga aacaaaaacu ccaggccaaa uucaguuaga gcgauaucag      480

accuacgggc agcugagagg agauuucaca gucgaaaagg acgguaaaaa gcauaggcuu      540

aucaacgugu uuccgaccag ugcauaucgg uccgaggccu ugcguauacu gcaaacucag      600

caggaguuca auccccagau uacagaugaa uuuaucaacc gcuaccuaga gauucucacc      660

ggcaagagaa aauauuacca cgggccugga aaugaaaagu cuaggacgga cuacggccgg      720

uaucgaacua gcggugagac ccuggauaac aucuucggga uccuuauugg aaaaugcaca      780

uuuuacccag acgaguuccg cgcugcaaag gccuccuaua cugcucaaga guuuaaccug      840

uugaaugacc ucaacaaucu gaccguaccc acagagacca aaaaguuauc aaaagaacag      900

aagaaccaga uaaucaauua cguuaagaac gagaaagcca ugggcccugc gaagcuguuc      960

aaauauauug caaagcuccu uaguugugau guggccgaca ucaaaggcua cagaauagau     1020

aagagcggga aggcugaaau ucauacguuu gaggccuaua ggaaaaugaa gacucuggaa     1080

acauuggaca ucgagcaaau ggaucgggag acccuagaca aacuggcaua cguccucacu     1140

cugaauacag aacgcgaggg aauccaggaa gcucuugagc acgaguucgc cgaugguucu     1200

uucucccaga aacaggugga cgagcuggug caauuuagaa aagcgaacuc aagcauuuuc     1260

ggcaaggggu ggcacaauuu uagugucaag uugaugaugg agcucauccc agaacuguac     1320

gagaccucug aagaacagau gacgauuuua accaggcugg gaaaacagaa gacaacuucc     1380

agcucgaaca aaaccaagua uauagaugag aaacuacuca cagaagagau cuacaauccc     1440

guuguggcca agucuguacg ucaggcaauu aagaucguga acgcugccau caaagaauau     1500

ggcgacuucg auaauauugu cauagagaug gcucgggaaa cuaacgagga cgacgagaag     1560

aaagccaucc aaaagauuca gaaagcaaau aaggaugaaa aagacgccgc uaugcugaag     1620

gcggcaaacc aguacaacgg uaaggccgag cuuccucauu ccguguuuca cgggcauaaa     1680

caguuggcua ccaagauccg acuguggcac caacagggcg aaagaugccu cuauacagga     1740

aaaaccauuu caauccacga ucugauaaau aacaguaauc aguucgaggu ugaccauauc     1800

cuuccacuga gcauuacuuu ugaugacucc uuggccaaca aggugcucgu cuacgcaacg     1860

gccaaucagg aaaaaggcca aaggacaccg uaucaggcuc uggauucuau ggacgaugcc     1920

uggagcuucc gcgaguuaaa ggcauuugug cgggagucaa agacccugag uaacaaaaag     1980

aaagaauacc uacucacuga ggaagacauc uccaaguucg auguaaggaa aaaguuuauu     2040

gagagaaauc ugguugacac ccgcuacgcg agccgagugg uccuuaacgc uuugcaggaa     2100

cacuuccggg cccauaagau cgauacaaaa gugucugucg ugcgugggca auuuaccuca     2160

cagcugagaa ggcacugggg aauagagaag acucgcgaca cauaucauca ccacgcaguu     2220

gacgcccuca uuaucgcugc caguagccag cugaauuuau ggaaaaagca gaaaaacacg     2280

cugguguccu acucugagga ucaacuuuug gacauugaaa ccggugagcu caucucggau     2340

gacgaauaua aggagagcgu auucaaggca cccuaccagc auuucgucga uacucugaaa     2400

uccaaggagu uugaagacuc aauacuauuc aguuaucagg uggauucuaa auuuaaucgg     2460

aagaucagcg acgcuacaau uuacgccacc agacaggcga aaguuggcaa ggauaaagcc     2520

gacgagacau augugcuggg gaagaucaag gauauuuaca cucaagacgg auacgaugcu     2580

uucaugaaaa ucuauaagaa agacaagucc aaauuucuca uguacaggca cgacccucag     2640

accuucgaaa aggucauaga gccaauucug gaaaacuauc ccaauaagca gaucaacgag     2700

aaaggcaagg aggugccuug uaauccauuu cuuaaauaca aggaggaaca ugguuauauc     2760

cgaaaauacu caaagaaggg gaacggcccc gaaauuaaga gcuugaagua uuacgauucu     2820

aaacugggaa accacaucga cauuacgccg aaggauucca auaacaaagu aguguuacag     2880

aguguuagcc cuuggcgcgc agacgucuac uucaauaaga ccacaggcaa guaugagaua     2940

cucgggcuga aauacgccga ucuucaauuu gaaaagggaa cugguaccua uaaaaucucu     3000

caggagaagu acaacgacau uaaaaagaag gagggcgugg auuccgacuc agaguucaaa     3060

uuuacacugu auaagaauga uuugcuccug gugaaagaca cugaaaccaa ggaacagcaa     3120

cuauuccggu uucugaguag aacgaugccc aaacagaagc acuacgucga gcucaaacca     3180

uaugauaagc agaaguucga agggggagag gcucugauca aaguucuugg caacguggcc     3240

aauagcgguc agugcaagaa aggguugggc aagucgaaca uuuccaucua caaagugagg     3300

acagacguac ugggaaauca acauauaauc aagaacgagg gcgacaagcc uaaauuagau     3360

uucuga                                                                3366


<210>  2554
<211>  2556
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2554
augaccaacg gcaagauccu ggggcucgac auuggagugg ccagcgucgg uguuggcaua       60

aucgaggcua aaacagggaa ggugauucac gcaaauucca gacuuuucuc ugccgcgaac      120

gcugaaaaua acgccgagag gcggggauuu cgcggcgcac gacgucugac uagaaggaaa      180

aagcaucggg uaaaacgcgu gagagauuug uucgaaaagu acgacaucuu aacggauuuu      240

aggaaucuga accuaaaucc cuaugagcuc cgggucaagg gucugaccga acagcuuaca      300

aacgaggagc uguucgccgc uuugcgcacu auugcaaaac gaagagggau cucauaccuc      360

gacgaugccg aagacgauag uaccggaagc uccgacuaug cuaagucuau agaugagaau      420

aggcggcugc ugaaaucgau gacaccuggc caaauccagc ucgaacguuu agagaaguac      480

ggccagcugc gcgggaacuu uaccguguau gacgagaaug gagaagccca cagacuuauu      540

aacguuuucu caacuagcga uuacaaaaau gaggcgagga agaucuugga aacacagagu      600

aacuauaaua agcaaauuac cgacgaguuu aucgaagauu acauagagau ucugacgcag      660

aaacggaagu acuaucaugg uccaggcaac gagaaauccc gaacugacua cgggcgcuuc      720

agaaccgacg gaacaacucu agaaaacauc uuuggcauuc ucaucgguaa gugcucuuuc      780

uauccggagg aauacagggc aagcaaagcc uccuauaccg cucaggaguu uaauuuccug      840

aacgaucuua auaaccugaa ggugcccaca gaaaccggga aauugucaac ggagcagaag      900

gaguaccucg ucaauuuugc caagaacagu aaaacucugg gagcaagcaa guuacugaaa      960

gaaaucgcua agcucgugga cggcgaugua aaagacauuu cuggcuaucg ggaggauucc     1020

aaggggaagc cugaccuuca cacauucgaa ccauaccgca aacugaaguu caauuugacc     1080

acuguugaua uagacaaccu aucaagagau auccuggaca aacucgccga uauucugaca     1140

cuuaauaccg agagggaagg aaucgaggac gcgauaaacc aaaaucugcc cgagcaguuu     1200

acgaaggaac agauuagcga gaucgugcag auccguaaaa gucaaucuuc cgccuucaac     1260

aaggguuggc auagcuuuuc ggcaaaguug augaaugaac ucauuccuga gcuguacgcu     1320

accucugagg aacagaugac aaucuuaacu cggcuggaga aauucaaggc caccaaaaag     1380

uccaguaaaa acacaaagac uauugacgaa aaggagauaa ccgaugaaau cuauaaucca     1440

gucguggcua aaucagugcg acagacaauu aagaucauca acgccgcagu caaaaaguac     1500

ggcgacuuug auaaaauugu uauagagaug cccagagaca agaacgccga ugacgagaaa     1560

aaguucaucg auaagaaaaa uaaggaaaac aaaaaggaga aagacgauag ccuaaagagg     1620

gcugcguauc ucuacaaugg gaccgacaag cugccugaug acguguuuca cggaaacaaa     1680

caacuugcaa cuaagauucg cuugugguau cagcagggcg aacggugucu guacuccggu     1740

aaaccaaucc ucauucagga ucugguacac aauucuaaca auuucgagau cgaccauaua     1800

cuuccgcuga gcuugucauu ugacgauagu cucgccaaca aggugcuggu cuaugcuugg     1860

gccaaucaag aaaaagagca gaagacgccc uaccagguga ucgacuccau ggaugcagcc     1920

uggagcuuca gagagaugaa ggacuacguu uuaaaacaga aggggauugg caaaaagaaa     1980

agggaauauc ugcuaacaac cgagaacauc gauaagauug aagugaagaa aaaguuuauc     2040

gagcgcaauc ucgucgacac ucgauacgcu ucucgggugg uacugaacuc acuucaaacc     2100

gccuugaaag aacugggaaa ggauacaaaa guuagugugg uccguggcca guucaccagc     2160

cagcucagaa ggaaguggaa aauagacaag ucccgcgaga cuuaucacca ucacgcagug     2220

gaugcgcuga uuaucgcugc cucuucgcag uuaaagcugu ggcaaaaaca cgagaaucuu     2280

auguuugaaa acuacgggga gaaucagguc gugaacaagg aaacaggaga gauuuugagc     2340

aucuccgacg augaguauaa agaaucaguu uuccagccuc cauaccaggg uuucgugaac     2400

acgauaaguu cuaaggcauu ugaggacgaa auccucuuca gcuaucaagu agauuccaaa     2460

uuuaaucgga aggucucaga cgccaccauu uacagcacua gaaaggcuaa acugggcaag     2520

gacaaaaagg aggaaacaua cgugcuaggg aaauga                               2556


<210>  2555
<211>  2598
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2555
augagacaca agaucggccu ggacauuggg auaaccagcg ugggaugggc cgucaucaac       60

cucgauauuc ccaggaucga ggaccuuggu guucggauuu ucgaucgcgc ugaaaauccu      120

cagacaggcg agucccuggc auugccacga cguuuagcca gaucugcgag gcggcgccug      180

agaaggcgga aacaucgccu agaacgaauc agaaggcucc ugauacggga ggggauccuu      240

acuaaggagg aacuggacaa auuguuugag gaaaagcacg agauugaugu guggcaacuc      300

cguguagaag cucuggaccg caaacugaac aaugaugagc ucgccagagu guuacugcau      360

cuugcaaaga ggcggggauu caagucaaac cgaaaaagug agcgcaauaa caaggaaaau      420

agcacgaugu ugaaacacau cgaggaaaac agagcccugc uauccggcua caggaccguc      480

gcugagauga uugugaagga cccgaaauuu gcauuccaua agcggaauaa gggugaaaac      540

uauacaaaua cuaucgcccg cgaugaccuc gagagagaga uaaaacugau uuuuaccaag      600

cagagggaau acgggaacau cguuugcaca gagaccuucg aaaaugagua uauuaugauc      660

ugggcuucuc agcgucccuu ugccucgaaa aacgauaucg aaaagaaagu gggauucugu      720

acuuuugagc cuaaggagaa acgggcgcca aaggcaacau acaccuucca gucauuuguc      780

gccugggaac acauuaauaa acuucgacug guguauccca cgggcacuag aggcuugacc      840

gacgaggaaa ggcgccuccu guacaaacag gcuuuccaca agaacaaaau aacauaucau      900

gauauccgga cuuuacugca ccucccugac gacaccuacu uuaaggggau uguauacgau      960

agaggagccc cacuuaaaca aagcgagaca aucagguucc uggaauugga cgcauaucau     1020

cagauacgca aggcuguuga uaagguguac gguaaaggca agaguuccuc uuuccuaccc     1080

auugacuuug auaccuucgg guaugcccug acgcucuuua aagacgaugc ggacauccga     1140

agcuaccugc ggaacgagua ugagcagaau ggaaagcgua ugccuaaccu ugccaauaaa     1200

gucuacgaua acgaaccaau cgaggaacug uugaaucucu ccuucacuaa guuuggccac     1260

cugucauuaa aggcacugag aaacauucua ccguauaugg agcaagguga gguguacagu     1320

guggcuugcg aaagggccgg guacacauuc accggaccca aaaagaaaca gaagacucuc     1380

cugcuuccua auaucccacc cauugcuaac ccugucguua ugcgcgccuu gacacaggca     1440

cggaaagugg uaaaugccau aaucaagaag uauggcagcc cagugucuau ucacaucgag     1500

cuggcuagag accucuccca gaccuuugau gaaaggcgaa aaacgaagcg cgagcaagac     1560

gaaaaccgga aaaagaauga gaccgcgauc agacagcuga uggaguacgg ccuuacaauu     1620

aaccccacug ggcaugacau agucaaauuc aagcuguggu cagaacagaa uggaaggugu     1680

gcauauagcu ugcaaccgau cgagauugaa cgucuccugg agccugguua cguggaaguu     1740

gaucacguga uccccuauag ucgcucuuua gacgauuccu acaccaacaa aguccuggug     1800

cuaacaaagg agaaucggga gaagggcaac aggauuccag ccgaauaucu cgggguagga     1860

acugagagau ggcagcaguu uaaaaccuuc guucugacaa acaagcaguu uagcaaaaag     1920

aaacgagacc ggcuuuugcg ccugcauuac gaugaaaaug aggaagcuga guggaagaac     1980

agaaaucuca acgacaccag guacaucucg cgguucuuug ccaauuucau acgagagcac     2040

cugaaguucg cagaaucuga ugacaaacaa aagguguaua cugucaacgg cagagugacg     2100

gcccauuuac gcuccaggug ggauuuuaau aaaaaccgug aggaaucaga ccugcaccac     2160

gcugucgaug ccgcaaucgu ggcgugcaca aguccuagcg acauugcucg gguuaccgcc     2220

uucuaccaga gaagggagca guguaaggag uccgcaaaga aggccgaacc ccauuuucca     2280

cagcccuggc cucacuucgc ugaugagcuu cgcgcccgau ugucuaagaa uccaaaagaa     2340

agcaucaagg cacucaaccu ggguaauuau gacgaccaaa aacuagaguc acugcagccg     2400

guguuuguaa guagaaugcc caagcggucc gucacugggg cugcccauca ggaaacccuc     2460

aggcgcuaca uuggcaucga ugagcguagc ggaaaaauac agacaguggu uaagaccaag     2520

cugucugaga uuaaacuuga cgaaacuggc cacuucccua uguaugggaa ggagucagau     2580

ccacccgcga gugccuga                                                   2598


<210>  2556
<211>  2886
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2556
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacaa uggagacgag      420

gaaaaaggca agauuuuaaa ggcaaucaaa cagaacgagg agaagcuggu uaauuaucag      480

agcgugggug aauaccuuua uaaagaguac uuucagaagu ucaaagaaaa caguaaggag      540

uuuauuaaug uaagaaacaa aaaggaaucc uacgagaggu guaucacuca aucuuucuug      600

aaguaugagc ugaaacuaau auuucagaag cagcgggagu ucggguuuag cuucuccaaa      660

aaguucgaag aagaggugcu cucagucgcc uuuuacaaac gugcucugaa ggauuucagu      720

caucuugugg gaaauugcag cuuuuucucu gacgaaaagc gcgccccgaa aaacucaccc      780

cuggcauuua uguucguugc uuugacgaga auuaucaauc uccugaacaa cuuaaagaau      840

accgagggca uucuguauac aaaagaugac cucaacgccc uucugaauga gguguugaag      900

aacggcacuc uaaccuacaa acagacaaag aagcugcucg ggcuguccga ugacuaugaa      960

uuuaaaggag agaaggguac cuacuucauc gaauuuaaaa aguauaaaga guucaucaag     1020

gcgcuuggcg aacacaaucu gagccaagac gauuugaacg agauugccaa ggacauaacu     1080

cucaucaaag augagauuaa gcugaaaaag gcauuagcua aauacgaccu gaaucagaac     1140

cagaucgaua gucuaucuaa gcucgaauuu aaagaccauc ugaauauagg guucaaggcc     1200

cuuaaguuga uuacaccucu gaugcucgag ggaaagaagu acgaugaggc uuguaacgaa     1260

cugaaucuua aagucgccau caacgaggac aagaaagauu uucugccagc auucaaugaa     1320

accuauuaca aggacgaggu gacgaacccc guaguuuuga gggccaucaa ggaauaucgg     1380

aaagugcuca augcucuguu aaagaaauac ggcaaggucc acaaaauuaa caucgagcug     1440

gcgcgagaag uggguaagaa ccacucccag cgcgcaaaga uugagaaaga gcaaaaugaa     1500

aacuauaagg ccaaaaagga ugcugagcua gaaugcgaga aacucgggcu gaagauaaau     1560

agcaagaaca uccuuaaauu gagacuguuc aaggaacaga aagaguuuug ugccuacucg     1620

ggagagaaga uuaaacucuc ugaccugcag gacgaaaaga uguuagagau cgaucauauc     1680

uauccuuacu ccaggucauu cgacgauagu uacaugaaua aagugcuggu cuuuacuaag     1740

caaaaccagg aaaaacuuaa uaaaacccca uucgaggcau uuggcaacga caauguuaag     1800

uggcagaaaa uugaaguguu ggccaagaac cuccccgaga aaaagcagaa gcggauacug     1860

gauaaaaauu auaaggacaa agagcaaaag gauuucaaag accgcaaccu aaaugauaca     1920

agauacaucg cuaggcuggu acucaacuau acuaaggacu accuggauuu ucuuccuuug     1980

agcgacgaug aaaauaccaa gcugaaaggc uccaaggugc acgucgaggc caaaucuggg     2040

auguuaacaa gcgcacuccg ucauaccugg ggauucucaa cgaaggaccg gaacaaccau     2100

cugcaccacg cgauugacgc ugccaucauu gcauaugcca auaacaguau cgugaaagau     2160

uuuuccgacu ucaagaagga acaggagagc aauucugcug agcuuuacgc caaaaagaua     2220

ucagaacugg auuauaaaaa caagcgaaaa uuuuucgagc cauuuagugg uuucagacag     2280

aagguuuugg acaagaucga ugaaauuuuc gugagcaaac cggagaggaa gaaacccucc     2340

ggcgcacucc acgaagagac uuuucgcaag gaggaagagu ucuaccaguc uuacggggga     2400

aaagaaggcg uccugaaggc ucuagagcug gguaaaaucc ggaaggugaa ugggaagauu     2460

guuaaaaacg gcgacauguu uagaguagau aucuucaagc auaaaaagac aaauaaauuu     2520

uaugccgugc cuauauacac cauggacuuc gcgcucaagg uccugccaaa caaggccgug     2580

gcuaggucga aaaagggaga aauuaaagau uggauccuua uggacgagaa uuaugaguuu     2640

ugcuucagcu uguacaagga uucccugauu uuaauccaaa cuaaagacau gcaggaaccc     2700

gagcucgucu auuuuaacgc auucacauca aguaccgugu cucugauagu uagcaagcac     2760

gauaauaagu uugaaacgcu uuccaaaaac cugaaggugu ucgagaaaua caucguauca     2820

gcccuaggcg aagucaccaa ggcugaguuc cgccagcgag aggacuuuaa aaaugaacuc     2880

cgguga                                                                2886


<210>  2557
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2557
auggccaaga uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaagacug cggagugaga auuuucacca aggccgagaa ucccaaaaca      120

ggugaaucac uugcgcugcc uaggcggauc gcucgcagug cccgaaagcg uuuggcaaga      180

aggaaagccc gguuaaacca ccugaagcau cuaauugcua augaguuuaa gcucaacuac      240

gaagauuauc agagcuucga cgagauccug gcaaaagccu acaagggcuc ccuuauaucu      300

ccauaugagc ugcgcuuuag agcuuugaau gaacuccugu cgaaacaaga uuucgccagg      360

gucauccugc acauugcgaa gcggcgcggg uacgacgaua ucaaaaacaa uggagacgag      420

gaaaagggca agauucucaa agcaaucaag cagaacgagg agaaauuagc caauuaucag      480

ucaguuggug aauaccugua uaaggaguac uuucagaaau ucaaggaaaa cagcaaagag      540

uuuauaaaug ugcgaaacaa gaaggaaagu uacgagagau guauugcuca auccuuccuu      600

aaagaugagu ugaagcugau cuuuaaaaag cagagggaau uuggguuuuc uuucagcaaa      660

aaguucgagg aagagguacu auccguggcc uuuuauaagc gggcacucaa agacuucuca      720

caucuggucg gaaauugcag uuucuucacu gaugaaaagc gugcuccgaa aaacagcccc      780

cuugccuuua uguucguggc gcugacgcgc auuaucaauu ugcucaacaa ccugaagaau      840

accuuauaca caaaagacga ucugaacgcc cuccuuaaug agguucugaa gaacggcacu      900

uugaccuaua agcagacaaa gaagcuacug ggccucucug acgacuacga guuuaaaggg      960

gaaaagggaa auuauuucau cgaguuuaaa aaguacaagg aguucauuaa agcacugggu     1020

gaacacuccc uuucacagga ugaccugaac gaaauagcua aggauaucac cuugauuaaa     1080

gacgagauca agcucaaaaa ggcccuggcu aaauaugauu uaaaugagaa ccaaauagac     1140

aaucugagca agcuagaauu uaaggaucau cucaacauua guuucaaagc ccugaaacuu     1200

aucacuccuu ugaugcugga gggcaaaaag uacgacgaag cauguaauga gcucacacug     1260

aaaguggcca ucaacgagga uaagaaggac uuucuuccag cuuucaauga aaccuacuau     1320

aaagaugagg ucacgaaccc cgugguacug agagcgauua aggaauacag gaaaguuuug     1380

aaugcacucc ugaagaaaua ugggaaggug cacaagauca acauugaguu agcccgggaa     1440

gucggcaaaa accacucuca gcgagcuaag auagagaaag agcagaauga aaacuacaag     1500

gccaaaaagg acgcagagcu ggaaugcgag aagcuaggac ucaaaaucaa uuccaagaac     1560

auucugaaac uucgcuuguu caaggaacag aaagaguuuu guguguauag cggugagaag     1620

aucaaacugu cggaccucca agaugaaaag augcuggaga ucgaccauau uuacccuuau     1680

ucuagauccu ucgaugacuc auacaugaau aagguguuag ucuuuacuaa acagaaccag     1740

gaaaagcuga aucaaacccc auucgaggcc uuugggaacg auagugcuaa auggcagaag     1800

auagaaguuc uugccaaaaa uuugcccaca aagaagcaga aaaggauccu cgacaagaac     1860

uacaaagaua aggagcagaa agacuucaag gaucggaauc ugaacgacac ucgcuauauu     1920

gcaaggcuag ugcugaauua caccaaggau uaucucgacu uucugccucu uagcgaugac     1980

gagaacacaa aauugaauga cacccaaaag ggauccaaag uacacgugga agcgaagucu     2040

ggcaugcuga cgagcgcuuu aagacauacu uggggcuucu caacaaaaga ucguaacaac     2100

caccuccacc augccaucga cgcagucauu aucgccuacg cuaauaacag uauagugaag     2160

gccuuuuccg auuucaagaa agagcaggaa agcaauaccg cagagcugua ugcuaagaaa     2220

aucucugagc uugacuacaa gaacaaacgg aaguuuuucg aaccauuuuc aggguuccga     2280

cagaagguuc uggauaaaau ugacgagauc uucgugagua agccggaaag gaaaaagccc     2340

agcggagccu ugcacgagga aacuuuuaga aaagaggagg aguucuauca guccuacggu     2400

ggcaaggaag ggguccucaa agcgcuggaa cuaggaaaga uucgcaaggu gaauggcaaa     2460

aucguaaaga acggugauau guuucggguu gacauauuca aacauaagaa aacaaauaag     2520

uuuuacgccg ugccuauuua uaccauggau uucgcucuga agguccuccc aaacaaagca     2580

guggccagau cuaagaaagg ggagaucaag gacuggauuc ugauggauga aaauuacgag     2640

uuuugcuucu cgcuuuauaa agacagcuug auccugauac aaacgaagga uaugcaggag     2700

cccgaauuug ucuacuauaa cgcuuucacc uccucaacag ugaguuuaau cguuucuaag     2760

cacgacaaua aauuugagac ucucagcaag aaccagaaaa uucuguucaa gaaugccaac     2820

gaaaaagagg ugaucgcaaa guccauuggc auccagaauc uuaagguauu cgaaaaauac     2880

auagucucag cgcugggaga ggugaccaag gccgaguuua ggcaacgcga agaguucaaa     2940

aacgaacuac gauga                                                      2955


<210>  2558
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2558
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacag acuaaaacac cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagguuucg ggcucucaau gaacugcugu caaagcaaga uuucgcccgc      360

gucauccucc auauagcgaa acgaagaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag caauuuuaaa ggccaucaaa cagaaugaag agaagcuggc uaacuaucag      480

aguguuggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugaggaauaa aaaggagucu uacgaacggu guauugccca aaguuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa cagcgugagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguacu caguguggca uuuuauaagc gcgcucugaa ggauuucagc      720

caccuugucg ggaacugcuc cuuuuucacg gacgagaaaa gagccccgaa aaauucuccc      780

cuggcguuua uguucguggc cuugaccagg auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa ucuuauacac uaaggaugac cucaaugcac uucugaacga guuuuugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucaga cgauuacgag      960

uucaaggggg aaaaaggaac uuauuuuauu gaguucaaga aauacaagga auuuaucaag     1020

gcucuuggug agcauaaccu gagccaggac gauuugaaug aaaucgccaa agacauuaca     1080

cucauaaagg augagaucaa acugaagaag gcuuuagcca aguaugaccu gaaccaaaau     1140

cagauugaua gucuaucuaa acucgaguuc aaggaccacc ugaacaucuc cuuuaaggca     1200

cuuaaauuga uaaccccucu gaugcucgaa ggcaagaaau acgaugaggc cuguaaugaa     1260

cugaaccuua agguugcuau uaaugaggac aaaaaggauu uccugccagc guucaacgag     1320

acguacuaua aggacgaagu gacuaauccc gucguguugc gggcaaucaa agaguaccga     1380

aagguacuca acgcccugcu gaaaaaguau gggaaaguuc auaagaucaa uauugaauua     1440

gcucgcgagg ugggaaagaa cuacagccag agagccaaaa ucgaaaagga gcagaacgag     1500

aauuauaaag caaagaaaga cgccgaacua gagugcgaaa agcucggccu gaagauuaac     1560

ucgaaaaaua uacuuaaguu gaggcuguuu aaagagcaaa aggaguucug ugcuuacucu     1620

ggugagaaaa ucaagauuuc cgaucuccag gacgagaaaa ugcuggaaau cgaucacauc     1680

uauccuuacu cacggaguuu ugacgauagc uacaugaaca aggucuuagu guucaccaag     1740

cagaaucaag agaaacugaa ccagacacca uuugaagccu ucgggaauga cuccgcaaag     1800

uggcagaaaa uugaggugcu ugcgaagaac uugcccacua aaaagcagaa gcgcauacuc     1860

gauaaaaauu auaaggacaa agaacaaaag gauuuuaaag acagaaaccu gaaugauacc     1920

agguacaucg cucgucuagu ccugaacuau acaaaggacu accucgauuu ccugccucuu     1980

ucugacgacg agaauaccaa guugaacgau acgcacaaag gaagcaaggu ucauguggag     2040

gccaaaucag gcaugcugac uagcgcauua cggcacacau ggggcuuuuc caccaaggac     2100

cgaaauaacc aucucagaca cgccauugau gcuguaauca uugccuaugc aaacaauagc     2160

aucgugaagg cuuucucuga cuuuaaaaag gaacaggagu caaacagugc cgaacuguac     2220

gcgaaaaaga uaagcgagcu ugauuauaaa aauaagagga aauucuuuga gccauucucc     2280

ggguuccgcc agaagguccu ggacaagauc gaugaaauuu uugugucuaa accggagcgg     2340

aagaaacccu cgggagccuu gcacgaagag acuuucagaa aggaagagga guuuuaccag     2400

agcuacggug gcaaagaagg gguucucaag gcucuggagc uaggaaaaau caggaaggug     2460

aacggcaaga uugucaaaaa uggugacaug uuccgcgugg auaucuuuaa gcauaaaaag     2520

acaaacaaau ucuaugcagu accuauauac accauggacu uugcccugaa gguucuccca     2580

aauaaggcug uggcccgauc caaaaagggg gaaauuaaag auuggauccu gauggacgag     2640

aacuaugagu ucugcuuuuc acuuuacaag gauaguuuga uucugaucca aacgaaagac     2700

augcaggaac ccgaguucgu cuauuacaau gcauuuaccu cuagcacagu guccuuaaua     2760

gucucaaagc acgacaacaa guucgaaacu cucagcaaaa aucagaagau ccuguucaaa     2820

aacgcgaaug agaaggaagu gauugccaaa ucuaucggca uucagaaccu uaagguuuuu     2880

gagaaguaua ucguguccgc ucugggagaa guaaccaaag ccgaguuccg gcaacgugag     2940

gauuuuaaga aauga                                                      2955


<210>  2559
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2559
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagucgagaa ucccaagaca      120

ggugaaucac uugcccugcc ucggcgcuug gcgcgaagug cucguaaaag auuagccagg      180

cggaaggcac gccugaacca ccuaaaacau cucaucgcca augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcuaaggcau acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcccucaau gaacugcugu caaagcaaga uuucgcucgg      360

guuauccucc acauagccaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag cgauuuuaaa ggcaaucaaa cagaaugaag agaagcuggc caacuaucag      480

agugugggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg uaagaaauaa aaaggagucu uacgaaaggu guauugcuca aucuuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa cagcgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaagugcu cagugucgcc uuuuauaagc gugcacugaa ggauuucagc      720

caucuugugg ggaacugcag cuuuuucacg gacgagaaac gcgcuccgaa aaauuccccc      780

uuagccuuua uguucguuac cuugacaaga auaauuaacc uccugaauaa ccugaaaaac      840

acugaaggaa uccuguacac caaggaugac cucaaugcgc uucugaacga gguguugaaa      900

aauggcacac uaaccuauaa gcagacuaag aaacugcucg gccugucaga cgauuacgag      960

uuuaaggggg aaaaaggaac auauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcccuuggug agcacaaccu gagccaggac gauuugaaug aaaucgcaaa agacauuacc     1080

cucauaaagg augagaucaa acugaagaag gcuuuagcca aguaugaccu gaaccaaaau     1140

cagauugaua gucuaucuaa acucgaguuu aaggaccauc ugaacaucuc cuucaaggcu     1200

cuuaaauuga uaacgccucu gaugcucgaa ggcaagaaau acgaugaggc cuguaaugaa     1260

cugaaccuua aggucgcaau uaaugaggac aaaaaggauu uucugccagc cuucaacgag     1320

acuuacuaua aggacgaagu gaccaauccc guaguuuuga gggcuaucaa agaguaccgg     1380

aaggugcuca acgcgcugcu gaaaaaguau gggaaagucc acaagaucaa uauugaauua     1440

gcacgagagg ugggaaagaa ccacagccag cgcgccaaaa ucgaaaagga gcagaacgag     1500

aauuacaaag cuaagaaaga cgccgaacua gagugcgaaa agcucggccu gaagauuaac     1560

ucgaaaaaua uacuuaaguu gagacuguuc aaagagcaaa aggaauuuug ugcauauucu     1620

ggugagaaaa ucaagauuuc cgaucuccag gacgagaaaa ugcuggaaau cgaucauauc     1680

uacccuuauu caaggaguuu cgacgauagc uacaugaaca agguguuagu cuuuacaaag     1740

cagaaucaag agaaacugaa caagacucca uucgaagccu uugggaauga cuccgcuaaa     1800

uggcagaaga uugagguucu ugccaaaaac uugcccacca agaagcagaa acggauacuc     1860

gauaagaauu acaaagacaa ggaacagaaa gauuucaagg accgcaaccu gaaugauaca     1920

agauauaucg caaggcuagu gcugaacuac accaaggacu aucucgauuu ucugccucuu     1980

ucugacgacg agaauacgaa auugaacgau acucaaaagg gaagcaaagu acacguggag     2040

gcgaagucag gcaugcugac aagugcuuua cgucauaccu ggggcuucuc cacuaaagac     2100

cggaauaacc accuccacca ugccauugau gcagucauca uugccuacgc uaacaauagc     2160

aucgugaagg ccuuuucuga cuucaagaaa gaacaggagu caaacagugc agaacuguau     2220

gcuaagaaaa uaagcgagcu ugauuacaag aauaaacgaa aguuuuucga gccauuuucc     2280

ggguucagac agaagguucu ggacaaaauc gaugaaauuu ucgugucuaa gccggagagg     2340

aaaaagcccu cgggagccuu gcacgaagag acauuucgca aagaagagga guucuaucag     2400

agcuacggug gcaaggaagg gguccucaaa gcgcuggagc uaggaaagau ccggaaggug     2460

aacggcaaaa uuguaaagaa uggugacaug uuuagaguug auaucuucaa acauaagaaa     2520

accaacaagu uuuacgccgu gccuauauau acgauggacu ucgcucugaa gguccuccca     2580

aauaaagcag uggccagguc caagaaaggg gaaauuaagg auuggauccu gauggacgag     2640

aacuacgaau uuugcuucuc acuuuauaaa gauaguuuga uucugaucca aaccaaggac     2700

augcaggagc ccgaguuugu cuacuauaau gcuuucacau cuagcacugu guccuuaaua     2760

guuucaaagc acgacaacaa auuugaaacc cucagcaaga aucagaaaau ccuguucaag     2820

aacgccaaug agaaagaagu gauugcaaag ucuaucggca uucagaaccu uaagguauuc     2880

gagaaauaca ucgucuccgc gcugggagaa gugacaaagg ccgaguuucg ccaacgagag     2940

gauuucaaaa aguga                                                      2955


<210>  2560
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2560
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag caauuuuaaa ggccaucaaa cagaaugaag agaagcuggc uaacuaucag      480

aguguuggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugagaaauaa aaaggagucu uacgaaaggu guauugccca auccuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa cagcgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguacu caguguggca uuuuauaagc gugcucugaa ggauuucagc      720

caucuugucg ggaacugcuc auuuuucacg gacgagaaac gcgccccgaa gaauagcccc      780

cuggcguuua uguucguggc cuugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa ucuuauacac uaaggaugac cucaaugcac uucugaacga gguuuugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucuga cgauuacgag      960

uuuaaggggg aaaaaggaac uuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcucuuggug accacagccu gagucaggau gacuugaacg agaucgccaa agauauuaca     1080

cucauaaagg acgaaaucaa acugaagaaa gcauuagcca aguaugaucu gaaucaaaac     1140

cagauugacu cucuauccaa acucgaguuu aaggaucauc ugaauaucag cuucaaggca     1200

cuuaaauuga uaaccccucu gaugcucgag ggcaagaaau acgacgaagc cuguaacgag     1260

cugaaucuua agguggcuau uaacgaagau aaaaaggacu uucugccagc guucaaugag     1320

acguacuaua aggacgaggu cacuaacccc gugguauuga gggcaaucaa agaauaccgg     1380

aagauccuca augcccugcu gaaaaaguau gggaaaguuc acaagauuaa caucgaguua     1440

gcucgagaag ugggaaagaa ucacucgcag cgcgccaaaa uugagaagga acagaacgag     1500

aacuacaaag caaagaaaga ugccgagaua gaaugcgaga agcuaggccu caagaucaau     1560

ucuaaaaaca uucugaaacu uagauuguuc aaagaacaaa aggaguuuug ugcuuauucc     1620

ggugaaaaga ucaagcuguc agaccuccag gaugagaaaa ugcuggagau cgaccauauu     1680

uacccuuaua guaggagcuu cgaugacucc uacaugaaua aggucuuagu guuuaccaag     1740

cagaaccaag aaaaacugaa uaagacacca uucgaggccu uugggaacga uucuacuaaa     1800

uggcagaaga uagaagugcu ugcuaaaaau uugcccgaga agaagcagaa acggauccuc     1860

gacaagaacu acaaagauaa ggaacagaaa gacuucaagg aucgcaaucu gaacgacacc     1920

agauauauug cgaggcuagu ccugaauuac acaaaggauu aucucgacuu ucugccucuu     1980

agcgacgaug agaacaccaa auugaaugac acgcaaaagg gaucaaaagu ucacguggag     2040

gcuaagagug gcaugcugac uuccgccuua cgucauacau ggggcuucag cgcaaaagau     2100

cggaacaauc accuccacca ugccaucgac gcugccauua ucgcauacgc uaacaacucu     2160

auaguaaagg ccuuuucaga uuucaagaaa gaacaggaga guaauagcgu ggaacuguau     2220

gcgaagaaaa ucuccgagcu ugacuacaag aacaaacgaa aguuuuucga gccauuuucu     2280

ggguucagac agaagguccu ggauaaaauu gacgaaaucu ucgugucgaa gccggagagg     2340

aaaaagccca gcggagccuu gcacgaagag accuuucgca aagaagagga guucuaucag     2400

uccuacggug gcaaggaagg gguucucaaa gcucuggagc uaggaaagau ucggaaggug     2460

aauggcaaaa ucgucaagaa cggugauaug uuuagagugg acauauucaa acauaagaaa     2520

acuaauaagu uuuacgcagu accuauuuau acaauggauu ucgcccugaa gguucuccca     2580

aacaaagcug uggccagguc aaagaaaggg gaaaucaagg acuggauucu gauggaugag     2640

aauuacgaau uuugcuucag ucuuuauaaa gacucuuuga uccugauaca aaccaaggac     2700

augcaggagc ccgaguuagu cuacuuuaac gcauucacga gcuccaccgu gucacucauc     2760

gucagcaagc acgauaauaa auuugaaaca cugucuaaga accagaaaau ucuuuucaag     2820

aaugcgaacg agaaagaagu gaucgccaag uccauuggca uccagaaucu gaagguuuuc     2880

gagaaauaua uagugagugc ucuaggagaa guaacuaagg ccgaguuucg ccaacgagag     2940

gacuucaaaa aguga                                                      2955


<210>  2561
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2561
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagucgagaa ucccaagaca      120

ggugaaucac uugcccugcc ucggcgcuug gcgcgaagug cucguaaaag auuagccagg      180

cggaaggcac gccugaacca ccuaaaacau cucaucgcca augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcuaaggcau acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcccucaau gaacugcugu caaagcaaga uuucgcucgg      360

guuauccucc acauagccaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag cgauuuuaaa ggcaaucaaa cagaaugaag agaagcuggc caacuaucag      480

agugugggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg uaagaaauaa aaaggagucu uacgaaaggu guauugcuca gagcuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa caacgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaagugcu cagugucgcc uuuuauaagc gugcacugaa ggauuucagc      720

caucuugugg ggaacugcuc uuucuuuacg gacgagaaac gcgcuccgaa gaauaguccc      780

cuggccuuua uguucguugc guugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa ucuuauacac uaaggaugac cucaaugccc uucugaacga gguguugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucaga cgauuacgag      960

uuuaaggggg aaaaaggaac uuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcacuuggug agcacaaccu gagccaggac gauuugaaug aaaucgcuaa agacauuaca     1080

cucauaaagg augagaucaa acugaagaaa gccuuagcua aguaugaccu gaaccaaaau     1140

cagauugaua gucuaucuaa acucgaguuu aaggaccauc ugaacaucuc cuucaaggcc     1200

cuuaaauuga uaaccccucu gaugcucgaa ggcaagaaau acgaugaggc auguaaugaa     1260

cugaaccuua aggucgccau uaaugaggac aaaaaggauu uucugccagc uuucaacgag     1320

acguacuaua aggacgaagu gacuaauccc guaguuuuga gggcgaucaa agaguaccgg     1380

aaggugcuca acgcacugcu gaaaaaguau gggaaagucc acaagaucaa uauugaauua     1440

gcccgagagg ugggaaagaa ccacagccag cgcgcuaaaa ucgaaaagga gcagaacgag     1500

aauuacaaag ccaagaaaga cgcagaacua gagugcgaaa agcucggccu gaagauuaac     1560

ucgaaaaaua uacuuaaguu gagacuguuc aaagagcaaa aggaauuuug ugccuauucu     1620

ggugagaaaa ucaagauuuc cgaucuccag gacgagaaaa ugcuggaaau cgaucauauc     1680

uacccuuauu caaggaguuu cgacgauagc uacaugaaca agguguuagu cuuuaccaag     1740

cagaaucaag agaaacugaa caagacacca uucgaagcau uugggaauga cuccgccaaa     1800

uggcagaaga uugagguucu ugcuaaaaac uugcccacua agaagcagaa acggauacuc     1860

gauaagaauu acaaagacaa ggaacagaaa gauuucaagg accgcaaccu gaaugauacc     1920

agauauaucg cgaggcuagu gcugaacuac acaaaggacu aucucgauuu ucugccucuu     1980

ucugacgacg agaauaccaa auugaacgau acgcaaaagg gaagcaaagu acacguggag     2040

gcuaagucag gcaugcugac uuccgccuua cgucauacau ggggcuucuc caccaaagac     2100

cggaauaacc accuccacca ugcaauugau gccgucauca uugcuuacgc caacaauagc     2160

aucgugaagg cauuuucuga cuucaagaaa gaacaggagu caaacagugc ugaacuguau     2220

gccaagaaaa uaagcgagcu ugauuacaag aauaaacgaa aguuuuucga gccauuuucc     2280

ggguucagac agaagguucu ggacaaaauc gaugaaauuu ucgugucuaa gccggagagg     2340

aaaaagcccu cgggagcguu gcacgaagag acuuuucgca aagaagagga guucuaucag     2400

agcuacggug gcaaggaagg gguccucaaa gcccuggagc uaggaaagau ccggaaggug     2460

aacggcaaaa uuguaaagaa uggugacaug uuuagaguug auaucuucaa acauaagaaa     2520

acaaacaagu uuuacgcugu gccuauauau accauggacu ucgcacugaa gguccuccca     2580

aauaaagccg uggcuagguc caagaaaggg gaaauuaagg auuggauccu gauggacgag     2640

aacuacgaau uuugcuucuc acuuuauaaa gauaguuuga uucugaucca aacgaaggac     2700

augcaggagc ccgaguuugu cuacuauaau gccuucaccu cuagcacagu guccuuaaua     2760

guuucaaagc acgacaacaa auuugaaacu cucagcaaga aucagaaaau ccuguucaag     2820

aacgcaaaug agaaagaagu gauugcgaag ucuaucggca uucagaaccu uaagguauuc     2880

gagaaauaca ucgucuccgc ccugggagaa gugaccaagg cugaguuucg ccaacgagag     2940

gauuucaaaa aguga                                                      2955


<210>  2562
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2562
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag caauuuuaaa ggccaucaaa cagaaugaag agaagcuggc uaacuaucag      480

aguguuggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugagaaauaa aaaggagucu uacgaaaggu guauugccca aaguuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa cagcgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguacu caguguggca uuuuauaagc gugcucugaa ggauuucagc      720

caucuugucg ggaacugcuc cuuuuucacg gacgagaaac gcgccccgaa aaauucuccc      780

cuggcguuua uguucguggc cuugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa ucuuauacac uaaggaugac cucaaugcac uucugaacga gguuuugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucaga cgauuacgag      960

uuuaaggggg aaaaaggaac uuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcucuuggug accacagccu gagucaggau gacuugaacg agaucgccaa agauauuaca     1080

cucauaaagg acgaaaucaa acugaagaag gcuuuagcca aguaugaucu gaaucaaaac     1140

cagauugacu cucuauccaa acucgaguuu aaggaucauc ugaauaucag cuucaaggca     1200

cuuaaauuga uaaccccucu gaugcucgag ggcaagaaau acgacgaagc cuguaacgag     1260

cugaaucuua agguggcuau uaacgaagau aaaaaggacu uucugccagc guucaaugag     1320

acguacuaua aggacgaggu cacuaacccc gugguauuga gggcaaucaa agaauaccgg     1380

aagguucuca augcccugcu gaaaaaguau gggaaagugc acaagaucaa cauugaguua     1440

gcucgagaag ucggaaagaa uuacucgcag cgcgccaaaa ucgagaagga acagaacgag     1500

aacuauaaag caaagaaaga ugccgagcua gaaugcgaga agcucggccu gaagauuaau     1560

ucuaaaaaca uacuuaaguu gagacuguuc aaagaacaaa aggaguuuug ugcuuacucc     1620

ggugaaaaga ucaaaauuuc agaccuccag gaugagaaaa ugcuggagau cgaccacauc     1680

uauccuuaca guaggagcuu cgaugacuuu uacaugaaua agguguuagu guucaccaag     1740

cagaaccaag aaaaacugaa ucagacacca uuugaggccu ucgggaacga uuccgcaaag     1800

uggcagaaaa uugaaguccu ugcgaagaau uugcccacua aaaagcagaa gcggauacuc     1860

gacaaaaacu auaaggauaa agagcaaaag gacuuuaaag aucgcaaucu gaacgacacc     1920

agauacaucg cuaggcuagu ucugaauuau acaaaggauu accucgacuu ccugccucuu     1980

ucugacgaug aaaacaccaa guugaaugac acgcagaaag gaagcaaggu gcauguagag     2040

gccaaaucag gcaugcugac uagcgcauua cgucacacau ggggcuuuuc cgccaaggau     2100

cggaacaauc aucuccacca cgcuauugac gccgugauca uugcauaugc uaacaacagc     2160

aucgucaaag ccuucucuga uuuuaagaag gagcaggaau caaauagugc ggagcuguac     2220

gccaaaaaga uaagcgaacu ugacuauaaa aacaagcgaa aauucuuuga gccauucucc     2280

ggguucagac agaaggugcu ggauaagauc gacgagauuu uuguuucuaa accggaaagg     2340

aagaaacccu cgggagcuuu gcaugaggaa accuuccgca aggaggaaga guuuuaccaa     2400

agcuacggug gcaaagaggg ggugcucaag gcacuggaac uaggaaaaau ccggaagguc     2460

aauggcaaga uugugaaaaa cggugauaug uucagaguag acaucuuuaa gcacaaaaag     2520

acuaauaaau ucuaugccgu uccuauauac acaauggauu uugcucugaa ggugcuccca     2580

aacaaggccg ucgcaagguc caaaaagggg gagauuaaag acuggauccu gauggaugaa     2640

aauuaugagu ucugcuuuuc acuuuacaag gacaguuuga uucugaucca gaccaaagac     2700

augcaggaac ccgaguucgu guauuacaac gcguuuacgu cuagcaccgu cuccuuaaua     2760

gugucaaagc augauaauaa guucgagaca cucagcaaaa accagaagau ccuguucaaa     2820

aaugccaacg aaaaggaggu uauugcuaaa ucuaucggca uucaaaaucu uaagguguuu     2880

gaaaaguaua ucguauccgc ccugggagag gucacuaaag cagaguuccg ccagcgagaa     2940

gacuuuaaga aauga                                                      2955


<210>  2563
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2563
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag caauuuuaaa ggccaucaaa cagaaugaag agaagcuggc uaacuaucag      480

aguguuggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugagaaauaa aaaggagucu uacgaaaggu guauugccca aaguuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa cagcgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguacu caguguggca uuuuauaagc gugcucugaa ggauuucagc      720

caucuugucg ggaacugcuc cuuuuucacg gacgagaaac gcgccccgaa aaauucuccc      780

cuggcguuua uguucguggc cuugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa ucuuauacac uaaggaugac cucaaugcac uucugaacga guuuuugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucaga cgauuacgag      960

uucaaggggg aaaaaggaac uuauuuuauu gaguucaaga aauacaagga auuuaucaag     1020

gcucuuggug agcacaaccu gagccaggac gauuugaaug aaaucgccaa agacauuaca     1080

cucauaaagg augagaucaa acugaagaag gcuuuagcca aguaugaccu gaaccaaaau     1140

cagauugaua gucuaucuaa acucgaguuc aaggaccauc ugaacaucuc cuuuaaggca     1200

cuuaaauuga uaaccccucu gaugcucgaa ggcaagaaau acgaugaggc cuguaaugaa     1260

cugaaccuua agauugcuau caaugaggac aaaaaggauu uccugccagc guucaacgag     1320

acguacuaua aggacgaagu uacuaauccc guggucuuga gggcaaucaa agaguaccgg     1380

aaggugcuca acgcccugcu gaaaaaguau gggaaaguac acaagauuaa uaucgaauua     1440

gcucgagagg uuggaaagaa ccacagccag cgcgccaaaa uugaaaagga gcagaacgag     1500

aauuacaaag caaagaaaga cgccgaacua gagugcgaaa agcucggccu gaagauaaac     1560

ucgaaaaaua uccuuaaguu gagacuguuu aaagagcaaa aggaguucug ugcuuauucu     1620

ggugagaaaa uuaagaucuc cgaucuccag gacgagaaaa ugcuggaaau cgaucauauu     1680

uacccuuauu caaggaguuu ugacgauagc uacaugaaca agguguuagu cuucaccaag     1740

cagaaucaag agaaacugaa ccagacacca uuugaagccu ucgggaauga cuccgcaaag     1800

uggcagaaaa uagaggugcu ugcgaagaac uugcccacua aaaagcagaa gcggauccuc     1860

gauaaaaauu acaaggacaa agaacaaaag aacuuuaaag aucgcaaucu gaacgacacc     1920

agauauauug cuaggcuagu gcugaauuac acaaaggauu aucucgacuu ccugccucuu     1980

ucugaugacg agaacaccaa guugaaugac acgcagaaag gaagcaaggu ccacguugag     2040

gccaaaucag gcaugcugac uagcgcauua cgucauacau ggggcuuuuc cgccaaggau     2100

cggaacaacc accuccacca ugcuaucgac gccgugauua ucgcauacgc uaauaacagc     2160

auaguaaaag ccuucucuga uuuuaagaag gaacaggagu caaauagugc ggaacuguau     2220

gccaaaaaga ucagcgagcu ugacuacaaa aacaagcgaa aauucuuuga gccauucucc     2280

ggguucagac agaaggugcu ggauaagauu gacgaaaucu uugucucuaa accggagagg     2340

aagaaacccu cgggagcuuu gcacgaagag accuuccgca aggaagagga guuuuaucaa     2400

agcuacggug gcaaagaagg ggugcucaag gcacuggagc uaggaaaaau ucggaagguu     2460

aauggcaaga ucgugaaaaa cggugauaug uucagagucg acauauuuaa gcauaaaaag     2520

acuaauaaau ucuacgccgu gccuauuuau acaauggauu uugcucugaa gguacuccca     2580

aacaaggccg uugcaagguc caaaaagggg gaaaucaaag acuggauucu gauggaugag     2640

aauuacgagu ucugcuuuuc acuuuauaag gacaguuuga uccugauaca gaccaaagau     2700

augcaggaac ccgaguucgu guacuauaac gcguuuacgu cuagcaccgu cuccuuaauc     2760

gugucaaagc acgacaauaa guucgaaaca cucagcaaaa accagaagau ucuguucaaa     2820

aaugccaacg agaaggaagu caucgcuaaa ucuauuggca uccaaaaucu uaagguguuu     2880

gagaaguaca uaguuuccgc ccugggagaa gugacuaaag cagaguuccg ccagcgagag     2940

gacuuuaaga aauga                                                      2955


<210>  2564
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2564
auggugagaa uccuggccuu cgacauuggc auaagcucca ucgggugggc uuuuucugag       60

aacgaugaac ucaaggacug cggagucagg auuuucacca aaguugagaa ucccaagaca      120

ggugaaucac uugcacugcc ucggcgcuug gcccgaagug cgcguaaaag auuagcuagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcaa augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gccaaggcuu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcacucaau gaacugcugu caaagcaaga uuucgcccgg      360

gugauccucc acauagcuaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag ccauuuuaaa ggcgaucaaa cagaaugaag agaagcuggc aaacuaucag      480

aguguaggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugagaaauaa aaaggagucu uacgaaaggu guauugccca aaguuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa cagcgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguccu caguguggcu uuuuauaagc gugcccugaa ggauuucagc      720

caucuuguug ggaacugcuc cuuuuucacg gacgagaaac gcgcaccgaa aaauucuccc      780

cuggcuuuua uguucguggc cuugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa ucuuauacac uaaggaugac cucaaugcgc uucugaacga ggucuugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucaga cgauuacgag      960

uuuaaggggg aaaaaggaac uuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcccuuggug agcacaaccu gagccaggac gauuugaaug aaaucgcaaa agacauuaca     1080

cucauaaagg augagaucaa acugaagaag gcuuuagcca aguaugaccu gaaccaaaau     1140

cagauugaua gucuaucuaa acucgaguuu aaggaccauc ugaacaucuc cuucaaggcu     1200

cuuaaauuga uaaccccucu gaugcucgaa ggcaagaaau acgaugaggc cuguaaugaa     1260

cugaaccuua agguggcaau uaaugaggac aaaaaggauu uucugccagc cuucaacgag     1320

acguacuaua aggacgaagu aacuaauccc guuguguuga gggcuaucaa agaguaccgg     1380

aagguccuca acgcgcugcu gaaaaaguau gggaaagugc acaagaucaa uauugaauua     1440

gcacgagagg ugggaaagaa ccacagccag cgcgccaaaa ucgaaaagga gcagaacgag     1500

aauuacaaag cuaagaaaga cgccgaacua gagugcgaaa agcucggccu gaagauuaac     1560

ucgaaaaaua uacuuaaguu gagacuguuc aaagagcaaa aggaauuuug ugcauauucu     1620

ggugagaaaa ucaagauuuc cgaucuccag gacgagaaaa ugcuggaaau cgaucauauc     1680

uacccuuauu caaggaguuu cgacgauagc uacaugaaca aggucuuagu uuuuaccaag     1740

cagaaucaag agaaacugaa caagacacca uucgaagccu uugggaauga cuccgcuaaa     1800

uggcagaaga uugaggugcu ugccaaaaac uugcccacua agaagcagaa acggauacuc     1860

gauaagaauu acaaagacaa ggaacagaaa gauuucaagg accgcaaccu gaaugauacc     1920

agauauaucg caaggcuagu acugaacuac acaaaggacu aucucgauuu ucugccucuu     1980

ucugacgacg agaauaccaa auugaacgau acgcaaaagg gaagcaaagu gcacgucgag     2040

gcgaagucag gcaugcugac uagcgcuuua cgucauacau ggggcuucuc caccaaagac     2100

cggaauaacc accuccacca ugccauugau gcagugauca uugccuacgc uaacaauagc     2160

aucguuaagg ccuuuucuga cuucaagaaa gaacaggagu caaacagugc agaacuguau     2220

gcuaagaaaa uaagcgagcu ugauuacaag aauaaacgaa aguuuuucga gccauuuucc     2280

ggguucagac agaaggugcu ggacaaaauc gaugaaauuu ucgucucuaa gccggagagg     2340

aaaaagcccu cgggagccuu gcacgaagag acuuuucgca aagaagagga guucuaucag     2400

agcuacggug gcaaggaagg ggugcucaaa gcgcuggagc uaggaaagau ccggaaggua     2460

aacggcaaaa uuguuaagaa uggugacaug uuuagagugg auaucuucaa acauaagaaa     2520

acaaacaagu uuuacgccgu cccuauauau accauggacu ucgcucugaa ggugcuccca     2580

aauaaagcag ucgccagguc caagaaaggg gaaauuaagg auuggauccu gauggacgag     2640

aacuacgaau uuugcuucuc acuuuauaaa gauaguuuga uucugaucca aacgaaggac     2700

augcaggagc ccgaguuugu guacuauaau gcuuucaccu cuagcacagu uuccuuaaua     2760

gugucaaagc acgacaacaa auuugaaacu cucagcaaga aucagaaaau ccuguucaag     2820

aacgccaaug agaaagaagu aauugcaaag ucuaucggca uucagaaccu uaaggucuuc     2880

gagaaauaca ucguguccgc gcugggagaa guuaccaagg ccgaguuucg ccaacgagag     2940

gauuucaaaa aguga                                                      2955


<210>  2565
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2565
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag caauuuuaaa ggccaucaaa cagaaugaag agaagcuggc uaacuaucag      480

aguguuggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugagaaauaa aaaggagucu uacgaaaggu guauugccca gagcuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa caacgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguacu caguguggca uuuuauaagc gugcucugaa ggauuucagc      720

caucuugucg ggaacugcuc auuuuucacg gacgagaaac gcgccccgaa aaauuccccc      780

uuagcguuua uguucguggc cuugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa uccuguacac uaaggaugac cucaaugcac uucugaacga gguuuugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucaga cgauuacgag      960

uuuaaggggg aaaaaggaac uuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcucuuggug accacagccu gagucaggau gacuugaacg agaucgccaa agauauuaca     1080

cucauaaagg acgaaaucaa acugaagaag gcuuuagcca aguaugaucu gaaucaaaac     1140

cagauugacu cucuauccaa acucgaguuu aaggaucauc ugaauaucag cuucaaggca     1200

cuuaaauuga uaaccccucu gaugcucgag ggcaagaaau acgacgaagc cuguaacgag     1260

cugaaucuua agguggcuau uaacgaagau aaaaaggacu uucugccagc guucaaugag     1320

acguacuaua aggacgaggu cacuaacccc gugguauuga gggcaaucaa agaauaccgg     1380

aagguucuca augcccugcu gaaaaaguau gggaaagugc acaagaucaa cauugaguua     1440

gcucgagaag ucggaaagaa uuacucgcag cgcgccaaaa ucgagaagga acagaacgag     1500

aacuauaaag caaagaaaga ugccgagcua gaaugcgaga agcucggccu gaagauuaau     1560

ucuaaaaaca uacuuaaguu gagacuguuc aaagaacaaa aggaguuuug ugcuuacucc     1620

ggugaaaaga ucaaaauuuc agaccuccag gaugagaaaa ugcuggagau cgaccacauc     1680

uauccuuaca guaggagcuu cgaugacucc uacaugaaua agguguuagu guuuaccaag     1740

cagaaccaag aaaaacugaa ucagacacca uucgaggccu uugggaacga uucugcaaag     1800

uggcagaaaa uugaaguccu ugcgaagaau uugcccacua aaaagcagaa gcggauacuc     1860

gacaaaaacu auaaggauaa agagcaaaag gacuucaaag aucgcaaucu gaacgacacc     1920

agauacaucg cuaggcuagu ucugaauuau acaaaggauu accucgacuu ucugccucuu     1980

agcgacgaug aaaacaccaa guugaaugac acgcagaaag gaucaaaggu gcauguagag     2040

gccaaaagug gcaugcugac uuccgcauua cgucacacau ggggcuucag cgccaaggau     2100

cggaacaauc aucuccacca cgcuauugac gccgugauca uugcauaugc uaacaacucu     2160

aucgucaaag ccuuuucaga uuucaagaag gagcaggaaa guaauagcgc ggagcuguac     2220

gccaaaaaga uauccgaacu ugacuauaaa aacaagcgaa aauuuuucga gccauuuucu     2280

ggguucagac agaaggugcu ggauaagauc gacgagauuu ucguuucgaa accggaaagg     2340

aagaaaccca gcggagcuuu gcaugaggaa accuuucgca aggaggaaga guucuaccaa     2400

uccuacggug gcaaagaggg ggugcucaag gcacuggaac uaggaaaaau ccggaagguc     2460

aauggcaaga uugugaaaaa cggugauaug uuuagaguag acaucuucaa gcacaaaaag     2520

acuaauaaau uuuaugccgu uccuauauac acaauggauu ucgcucugaa ggugcuccca     2580

aacaaggccg ucgcaagguc uaaaaagggg gagauuaaag acuggauccu gauggaugaa     2640

aauuaugagu uuugcuucag ucuuuacaag gacucuuuga uucugaucca gaccaaagac     2700

augcaggaac ccgaguuugu guauuacaac gcguucacga gcuccaccgu cucauuaaua     2760

gugagcaagc augauaauaa guuugagaca cucucuaaaa accagaagau ccuguucaaa     2820

aaugccaacg aaaaggaggu uauugcuaaa uccaucggca uucaaaaucu uaagguguuc     2880

gaaaaguaua ucguaagugc ccugggagag gucacuaaag cagaauuucg ccagcgagag     2940

gacuucaaga aauga                                                      2955


<210>  2566
<211>  2958
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2566
auggccaaga uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuguggag       60

aacgaugaac ucaaagacug cggagucaga auuuucacca aggccgagaa ucccaaaaca      120

ggugaaucuc uugcgcugcc uaggcgguug gcucgcucag cccgaaagcg uuuagcaaga      180

aggaaagccc ggcugaacca ccuaaagcau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agaguuucga cgagagccuu gcaaaagccu acaagggcac ucugauuucc      300

ccauaugagu ugcgcuuuaa agcucucaau gaacugcugu cuaagcaaga ucucgccaga      360

guuaucuuac acauagcgaa aaggcggggg uacgacaaca ucaaaaauuc ggaugacaag      420

gagaaaggag caauucugaa ggccgugaaa gaaaacgagg agaaacuugc uaauuauaag      480

ucaguaggcg aauacuugua uaaggaguac uuccagaaau uuaaggaaaa cagcaaggag      540

uucaucaaug ugcgcaacaa aaaggaaagu uacgagcgau guauugccca guccuuucug      600

aaagaugagc uaaagcucau cuuccagaaa caaagagaau uugguuucaa uuuuucuaag      660

aaguucgagg aagagguccu gagcguggca uucuauaaaa gggcucuuaa ggacuuuucc      720

caucugguug ggaacugcuc auucuuugug gaugaaaaac gggccccgaa gaauaguccc      780

uuggcguucg aguuugucgc ccucacgcgu auagugaacc uguuaaacaa ucugaacaaa      840

aauaacagcu acauuaagga cgaucucaau gcacuucugu cugagguauu gaagaacgga      900

acccuaacau auaaacagac uaagaaaauc cugggccucu ccgacgacua cgaauuuaag      960

ggccagaaag ggaauuauuu cauugaguuc aagaaguaca aagaauuuau caaggcucug     1020

ugugagcaca accuuaauca ggaugaccug aacgaaaucg ccaaagauau uaccuugguu     1080

aaggacgaga caaaacucaa gaaagcucug gccaaguaug auuuaaauca aaaccagaua     1140

gagaaucugu caaagaucga guucaaagac caucuaaaca uuagcuuuaa ggcacucaaa     1200

cugaucgugc cucuuaugcg cgaaggaaag aaauacgaug aagucugcaa ugaguugaac     1260

cugaagaccg ccauaaacga ggacaagaaa gaguuccucc cagcuuuuaa ugaaacuuac     1320

uauaaggaug aagugaacaa ucccgugguc cugagagcga uuaaagagua caggaagguu     1380

cuuaacaguc uguugaaaaa guaugguaag gugcacaaaa ucaauaucga acucgcacgg     1440

gagguaggca agaaccaccg agagcgcucu aaaauugaaa aggagcagaa ugaaaacuuc     1500

aaagccaaga aggacgcuga gcuggaaugu gagaaauuag ggcugaagau caauuccaaa     1560

aacauucuaa agcucagacu guucaaagag cagaaggaau uuugcgugua cagcggagag     1620

aaaauaaaga ucucggaucu ucaagacgaa aagauguugg agauugacca uaucuauccu     1680

uacucuaggu ccuucgauga cucauauaug aauaaagucc ugguguuuac aaagcagaac     1740

caggaaaaac ucaauaagac cccauucgag gccuuuggca aagaugacgu uaaguggcaa     1800

aagaucgaga cgcuggcaaa gaacuuaccc gccaagaaac agaagcggau ucuggauaaa     1860

aauuacaagg acaaggaaca gaaagauuuc aaggaccgca accuuaacga uacuagauac     1920

auagcuaggu uggugcucaa uuauaccaaa gacuaccugg aguuucuacc ucugagugau     1980

gacgaaaaca ucaagcucaa ugauacacag aaagguagca agguccacgu ggaggccaag     2040

uccgggaugc ugacuucugu acuucgucau accuggggau ucagcacaaa agaccggaac     2100

aaucacuugc accaugcaau ugacgcgguu aucauugcuu augccaacgc aucagugguc     2160

aaggccuuua gugauuucaa aaaggagcaa gaaaauuaca ccgcugagcu guaugccaaa     2220

aagaucaacg aauuagacuu uaagaauaaa cgaaaguucu uugagccauu cuccaacuuc     2280

agacagaaag ugcucgaaaa gauagaugag aucuuuguca gcaaaccgga gaggaagaaa     2340

cccucuggcg cacugcacga agagacguuc uacaaggaag aggaauuuua uaaggaguac     2400

ggcgggaaag agggagugcu uaaggcucug gaauugggua aaauucgcaa gguuaauggc     2460

aaaaucgugu caaacgccaa uauguuccgg guagacauuu uuaaguacaa gaaaacuaac     2520

aaguucuaug cggucccuau cuacacaaug gauuuugccc ucgggauacu gccaaauaaa     2580

gcugugguug gaggcaagga caaacagggu auuaucaagg auuggcuacu gauggacgag     2640

aacuaugagu ucuguuuuag ucucuacaag gauagccuga uucuuuugca gaccaaagac     2700

augcaagaac ccgaacuggu guauuucaag uccuuuaaau cuucgacugc aggguuaguc     2760

gugagcaagc augauaacaa auucgagaau cucaacgaga aucagaagaa gcuguucaaa     2820

uccgccaacg aaaagaaagu aguggcugag ggcaucggaa uacagaaucu uaagguuuuu     2880

gaaaaauaca ucgucucagc ccugggcgag auuacaaagg cagagaucaa agaaagagag     2940

aacuucaaga agaaauga                                                   2958


<210>  2567
<211>  2961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2567
augcccagcg acccugccga ggcuaagcca cuggaacucc uugauccgua cgcacugaga       60

gcccagggcu uggaccaagc guuaccccug acccacuucg ggagggcucu auuucaucuc      120

aaccagcggc gcggauucaa auccaaucga aagacagauc guggugacaa cgagaauggc      180

aaaaucaagg augccucuca gagacuggac ugggcaaugc acgaagccaa agcuaggacu      240

uauggggagu uucuucaucu gcggcgccag aaggcaccug auccaagaca agugcccacg      300

gucaggaccc gguugucagu uggaaagcgc ggcgguccug acgccaaaga agaggcuggg      360

uacgauuucu auccagaccg aagacaccuc gaggaagagu uucaugaacu guggagugcc      420

caggcgcagu uccaccccga gcugacacag gaucucaggg acuuaguguu ugagaagauu      480

uucuaccaac ggccucugaa agaaccaaag guaggacuuu gcuuguuuac uagcgaggaa      540

cgucuggcaa aagcccaccc gcuaacccag cgcagagugc ucuaugagac agucaaccag      600

cugaggauaa ccgcugaugg ccgguccgug cgaccccuuu cucgcgaaca gagagaccaa      660

aucauucugg ccuuggacac uaagaagccu acaaaaucgc ucaauucaau gguugugacc      720

cugcaggcau uaggcaaggu ccugaaacuc agggaggggg agcgguucac gcuugaaagc      780

ggagugcgcg aggcuaucgc cugugaucca guaagagcgg ccaugcuggc aacuuuuggu      840

cccaggugga gugcuuugga cuggccugaa caguggcaag uuauuucccg ugugcggaag      900

cuacaggaug acaaagauuu cgccgagcug gucgaauggc ucaucgagcu guacggcgug      960

gacaaggagg cugccgugaa aauagcaaac acccaucuuc cagaagggua uggacgacug     1020

ggcuugacag ccacuaccag aauccucgau aggcugaagu cuaaugucuu uacauacgcu     1080

caggcgguug cagccugcgg uuggcaccau agcgaccagc gcaccgggga guguuuagau     1140

cggcugcccu acuauggagc ugugcuagac agacacguaa uuccgggcac guacgaugaa     1200

aacgacgacg auaucacuag guucggccgc auuacaaauc cuaccgugca caucgggcuc     1260

aaccaacugc gacggcuugu caauaagaua auugaggugu auggaaaacc caaccagauc     1320

guuguggaau uggcccguga gcugaagcag uccgagcagc agaaaaauga cgcaauuaag     1380

cucaucagag auacuacaac cgccgcuaaa cagaggucag aaaagcugga ggaacuuggu     1440

aucacggaca acggccgcaa ucggaugcug uugagacucu gggaggaucu gaacccagac     1500

gaugccauga ggcgauucug cccuuacacc gggcagcgca uuagugcagc gaugauauuu     1560

gacggaagcu gugaugucga ccauaucuua cccuauucuc ggacacugga ugacggcuuc     1620

gcuaacagaa cucuaugccu cagggaggcc aaucgugaaa agcgcaacca gaccccaugg     1680

gaggcauggg gugggacacc caauugggaa gccauugaga cuaaccugaa aaaucuuccu     1740

gaaaacaagc gguggagauu ugcuccagau gccauggcaa gguucgaggg cgagaaugac     1800

uuuuuggacc gagcucuggu ggauacccaa uaccucgccc ggaucucccg cacauaucug     1860

gacaccuuau ucacugaagg aggccacgua uggguugugc cggggagacu gacggagaug     1920

cuuaggcggc auuggggauu gaacucacuc cugacagaua aagaccgagg ugcggucaag     1980

agcaaaaaua gaaccgauca ccgccaucac aguauagacg ccgcuguggu cgcagccacu     2040

gauaggucuc uacugaaccg uauuucccgg gcugccggcc agggggaagu ggcaggacag     2100

agcgcggagc ucaucgccag agaaacaccc ccuccauggg agggcuuuag gaaugaccug     2160

aagucgcagc uuggucgcau cauuguuucu caccgagcug aucaugggag aaucgaccgg     2220

caagccagga agcagggccg cgauuccacc gcaggacagu ugcaccaaga gaccgcuuac     2280

ucaauugugg acgauacucg uguagccagu agaauagacu uccugagcgu caaacccgca     2340

caguuacucg accagccugg ccgguccggg caagugaggg aucugcaacu ucgcaaggcc     2400

cugcgaguug cuacaggaga cuugacgggu aaagauuacg aaaaggcgcu cagagacuuu     2460

gccgcaaaac caggccccua uaaggggauc cggagggugc gcauuaucaa gccgcugcag     2520

gagcaggcua gagucccugu gccaguacua gaucccauca aagccuacca gggaggcucu     2580

aaccaugugu ucgaaauuug gcaucugccu gacgguaagg uugagcccca agucauaacc     2640

agcuuugaag cccacgcucu cgagguggag aaaaggccac acccugcagc caagcggcug     2700

cuucgugugc acaaagggga uauggucgcg uugaagcgcg aaggccgaac uguuguggga     2760

caugugcagc agaugcacau caaaaauggc cuguucauug uaccccauaa cgaggcuaau     2820

gccgacgcaa gaaacucaga uaggacagac ccauuuaagu ggauccagau ugccgcucaa     2880

ccggcaaucg ccgcggggau acggcgcguc gcuguggaug aaaucggaag auuaagggac     2940

ggcggugcca agaccauuug a                                               2961


<210>  2568
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2568
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug agucgaagcg cucguaaaag auuagccagg      180

cggaaggcac gccugaacca ccuaaaacau cucaucgcca augaguuuaa gcugaacuac      240

gaagauuauc aguccuucga cgagucucuu gcuaaggcau acaaaggcuc gcugauuuca      300

ccauaugagu ugagauuuag ggcccucaau gaguuccuga gcaagcaaga uuuugcucgg      360

gucauccugc acauagccaa acgccgaggg uacgacgaua ucaagaacaa uggagacgag      420

gaaaaaggcg agauucucaa ggcgaucaag cagaacgagg aaaaauuagu uaguuaucag      480

uccgugggug aguaccugua uaaggaauac uuccagaaau uuaaggaaaa uucuaaagag      540

uucauuaacg uaagaaauaa gaaagaaagc uacgagaggu guaucgcaca auccuuucuu      600

aaggaugaau ugaagcugau auuccagaaa cagcgggagu uuggguucuc auucaguaag      660

aaauuugaag aggaagugcu aagcgucgcc uucuauaagc gugcucucaa agacuuuucu      720

caucuggugg gaaacugcuc cuucuuuacu gaugagaagc gcgccccgaa aaauucaccc      780

cuugcauuca uguuuguugc ucugacgaga auuaucaacu ugcucaauaa ccugaaaaac      840

accgagggca uuuuauacac aaaggacgau cugaauacuc uccuuaacga agugcugaaa      900

aauggcaccu ugacauauaa gcagaccaaa aagcuacugg ggcucagcaa cgacuacgag      960

uucaagggag aaaaagguac uuauuuuauc gaguuccaaa aguacaaaga auuugucaag     1020

gcccugggcg agcacaaucu uagucaggac gaucugaacg agaucgcgaa agacauuaca     1080

uugauaaagg augaaaucaa gcucaaaaag gcccuggcaa aauaugacuu aaaucagaac     1140

cagauugauu cucuguccaa gcuagaguuc aaagaccauc ucaauaucag cuuuaaggcu     1200

cugaaacuua uaaccccuuu gaugcuggaa gggaagaagu acgaugaggc cuguaacgag     1260

cucaaucuga aaguggcuau uaacgaagac aagaaagauu uccuuccagc cuucaaugag     1320

acguacuaua aggacgaagu aacuaacccc guugugcuga gggcaaucaa agaguaccgg     1380

aaggucuuga augcccuccu gaagaaauau ggaaaggugc acaaaaucaa cauugaauua     1440

gcucgagagg ugggcaagaa ccacucgcaa cgcgcgaaaa ucgagaagga acagaaugag     1500

aacuacaagg caaagaagga ugccgaacug gagugcgaaa aacuaggucu caagauuaau     1560

ucuaaaaaca uacugaaacu uagauuguuu aaggagcaga aagaguucug ugucuauucc     1620

ggggaaaaga ucaaaauuuc agaccugcaa gacgagaaga ugcucgaaau cgaucauauc     1680

uacccuuaua guaggagcuu ugacgauucc uacaugaaua aaguucuggu guucaccaag     1740

cagaaccagg agaaauuaaa uaagacacca uuugaaacuu ucggaaacga ccggguaaag     1800

uggcagaaaa uugaggugcu ggcuaagaau cuucccacca aaaagcaaaa acgcauauug     1860

gauaagaacu acaaggacaa agagcagaag gauuuuaaag acagaaaucu caacgauaca     1920

agguauaucg cccgucuggu ccuaaauuac accaaggacu aucuggauuu ccucccucug     1980

ucugacgaug aaaacacgaa acuuaaugac acucagaagg gcagcaaggu gcacguugag     2040

gcaaaaucag gcauguugac aaguacccug cggcauacuu ggggguuuuc cacaaaggac     2100

cgagauaacc acuuacacca ugccauugac gcugccauca uugcauacgc gaacaauagc     2160

aucgugaagg cuuucucuga uuuuaagaaa gaacaggagu caaacaccgc cgagcucuau     2220

gcaaagaaga uaagugaacu ggacuacaaa aauaagagaa aauucuuuga gccauucagc     2280

ggauucaggc aaaaacuucu ggauaagauc gacgaaauuu uugucuccaa gccggagcgc     2340

aagaaacccu cuggugccuu gcacgaagag acguuccgga aggaggaaga guuuuaucag     2400

ucguacggcg ggaaagaagg agugcucaag gcucuggagc uaggcaaaau cagaaaggua     2460

aacgguaaaa ugguuaagaa uggggauaug uucagggugg acauuuuuaa gcauaaaaag     2520

accaacaaau ucuacgccgu cccuaucuau acaauggauu uugcacugaa ggugcuccca     2580

aauaaagcug ucgugcaggg caaggacaag aaaagcggac ugauaaagga uuggauucuu     2640

auggacgaaa acuacgaguu cugcuuuucc uuguauaaag auucacugau cuuaauucag     2700

acuaaggaca ugcaagagcc cgaacucguu uacuaugagc gccuggacag uucuaaugcc     2760

agccuuaccg uguccaaaca cgauaacaag uucgaaacac ugucaaagaa ucagaaaauc     2820

cuauuuaaga acgcgaauga gaaagaagua auagccaaga gcaucggcau ucagaaccuc     2880

aaagucuucg agaaguacau cgugucugcu cugggggagg uuacuaaggc agaguuccga     2940

cagcgggaag acuuuaaaaa guga                                            2964


<210>  2569
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2569
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag caauuuuaaa ggccaucaaa cagaaugaag agaagcuggc uaacuaucag      480

aguguuggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugagaaauaa aaaggagucu uacgaaaggu guauugccca gagcuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa caacgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguacu caguguggca uuuuauaagc gugcucugaa ggauuucagc      720

caucuugucg ggaacugcuc auuuuucacg gacgagaaac gcgccccgaa aaauuccccc      780

uuagcguuua uguucguggc cuugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa uccuguacac uaaggaugac cucaaugcac uucugaacga gguuuugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucaga cgauuacgag      960

uuuaaggggg aaaaaggaac uuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcucuuggug accacagccu gagucaggau gacuugaacg agaucgccaa agauauuaca     1080

cucauaaagg acgaaaucaa acugaagaag gcuuuagcca aguaugaucu gaaucaaaac     1140

cagauugacu cucuauccaa acucgaguuu aaggaucauc ugaauaucag cuucaaggca     1200

cuuaaauuga uaaccccucu gaugcucgag ggcaagaaau acgacgaagc cuguaacgag     1260

cugaaucuua agguggcuau uaacgaagau aaaaaggacu uucugccagc guucaaugag     1320

acguacuaua aggacgaggu cacuaacccc gugguauuga gggcaaucaa agaauaccgg     1380

aagguucuca augcccugcu gaaaaaguau gggaaagugc acaagaucaa cauugaguua     1440

gcucgagaag ucggaaagaa uuacucgcag cgcgccaaaa ucgagaagga acagaacgag     1500

aacuauaaag caaagaaaga ugccgagcua gaaugcgaga agcucggccu gaagauuaau     1560

ucuaaaaaca uacuuaaguu gagacuguuc aaagaacaaa aggaguuuug ugcuuacucc     1620

ggugaaaaga ucaaaauuuc agaccuccag gaugagaaaa ugcuggagau cgaccacauc     1680

uauccuuaca guaggagcuu cgaugacucc uacaugaaua agguguuagu guuuaccaag     1740

cagaaccaag aaaaacugaa ucagacacca uucgaggccu uugggaacga uucugcaaag     1800

uggcagaaaa uugaaguccu ugcgaagaau uugcccacua aaaagcagaa gcggauacuc     1860

gacaaaaacu auaaggauaa agagcaaaag gacuucaaag aucgcaaucu gaacgacacc     1920

agauacaucg cuaggcuagu ucugaauuau acaaaggauu accucgacuu ucugccucuu     1980

agcgacgaug aaaacaccaa guugaaugac acgcagaaag gaucaaaggu gcauguagag     2040

gccaaaagug gcaugcugac uuccgcauua cgucacacau ggggcuucag cgccaaggau     2100

cggaacaauc aucuccacca cgcuauugac gccgugauca uugcauaugc uaacaacucu     2160

aucgucaaag ccuuuucaga uuucaagaag gagcaggaaa guaauagcgc ggagcuguac     2220

gccaaaaaga uauccgaacu ugacuauaaa aacaagcgaa aauuuuucga gccauuuucu     2280

ggguucagac agaaggugcu ggauaagauc gacgagauuu ucguuucgaa accggaaagg     2340

aagaaaccca gcggagcuuu gcaugaggaa accuuucgca aggaggaaga guucuaccaa     2400

uccuacggug gcaaagaggg ggugcucaag gcacuggaac uaggaaaaau ccggaagguc     2460

aauggcaaga uugugaaaaa cggugauaug uuuagaguag acaucuucaa gcacaaaaag     2520

acuaauaaau uuuaugccgu uccuauauac acaauggauu ucgcucugaa ggugcuccca     2580

aacaaggccg ucgcaagguc uaaaaagggg gagauuaaag acuggauccu gauggaugaa     2640

aauuaugagu uuugcuucag ucuuuacaag gacucuuuga uucugaucca gaccaaagac     2700

augcaggaac ccgaguuagu guauuuuaac gcguucacga gcuccaccgu cucacucaua     2760

gugagcaagc augauaauaa guuugagaca cugucuaaaa accagaagau ccuuuucaaa     2820

aaugccaacg aaaaggaggu uauugcuaaa uccaucggca uucaaaaucu gaagguguuc     2880

gaaaaguaca ucguaagugc ccuaggagag gucacuaaag cagaauuucg ccagcgagag     2940

gacuucaaga acgagcuccg guga                                            2964


<210>  2570
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2570
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacaa uggagacgag      420

gaaaaaucag agauuuuaaa ggcaaucaag cagaacgagg aaaaacuggu uaauuaucag      480

agugugggcg aguaccuuua uaaggaauac uuucagaaau ucaaggagaa cuccaaagaa      540

uuuauuaaug uaagaaacaa gaaagagucu uacgagaggu guaucgccca aucuuucuug      600

aaggaugaac ugaagcuaau auuucagaaa cagcgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaagugcu cagugucgcu uuuuauaagc gugcccugaa agacuucagc      720

caucuugugg ggaauugcag cuuuuucacu gaugagaagc gcgcaccgaa gaacuccccc      780

uuaguuuuua uguucguggc uuugacgaga auuaucaauc uccugaacaa ccugaaaaau      840

accgagggca uucuguacac aaaggacgau cucaacgccc uucugaauga agucuugaaa      900

aacggaacuc uaaccuauaa gcagacaaag aagcugcucg gccugucaga cgacuacgag      960

uuuaaggggg aaaaaggaac cuauuucauc gaguuuaaga aauacaagga guucaucaaa     1020

gcgcuugaug aacacagccu gagucaagac gauuugaaug agauugccaa ggacauaacu     1080

cucaucaagg augaaauuaa acugaagaaa gcauuagcua aguaugaccu gaaagagaac     1140

cagaucgauu cucuauccaa gcucgaauuu aaagaccauc ugaauauuag cuucaaggcc     1200

cuuaaguuga uaacaccucu gaugcucgag gguaaaaagu acgaugaggc uuguaacgaa     1260

cugaaucuua aaguggccau caacgaggac aagaaagauu uucugccagc auucaaugaa     1320

accuacuaua aggacgaggu aacgaacccc guuguguuga gggccaucaa ggaauaccgg     1380

aaaauucuca augcucuguu aaagaaauau ggcaaggucc acaaaaucaa cauugagcug     1440

gcgcgagagg uggggaagaa ucacucgcag cgcgcaaaga uagaaaaaga gcagaacgaa     1500

aacuacaagg ccaaaaagga cgcugagcua gaaugcgaga aacucggacu gaagaucaau     1560

ucuaagaaca uucuuaaauu gagacuguuc aaggagcaaa aagaauuuug uguguauucc     1620

ggcgagaaga ucaaacucuc agaucugcag gacgaaaaga uguuagagau cgaucauauu     1680

uacccuuaua guaggagcuu cgacgauucc uacaugaaua aaguccuggu uuuugccaag     1740

cagaaccaag aaaaacuuaa uaaaacucca uucgaggcau uugguaacga cucuaccaag     1800

uggcagaaaa uagagguguu gacaaagaau cuccccacua aaaagcagaa gcggauccug     1860

gauaaaaacu acaaggacaa agaacagaag gauuucaaag accgcaaucu aaacgauacc     1920

agauauauug ccaggcuggu acucaauuac acaaaggacu aucuggauuu ucuuccuuug     1980

agcgacgacg agaacaccaa gcugaaugau acgcaaaaag ggucaaaggu gcacgucgaa     2040

gcuaaaagug gaauguuaac uuccgcccuc cgucauacau ggggcuucag caccaaggac     2100

cggaacaauc aucugcacca cgcaaucgau gcggugauua ucgcuuacgc caacaacucu     2160

auaguuaaag cauuuucaga cuucaagaag gagcaggaga guaauagcgc cgaacuuuau     2220

gcuaaaaaga ucuccgagcu ggauuacaaa aacaagcgaa aauuuuucga accauuuucu     2280

ggcuucagac agaagguguu ggacaagauu gaugagaucu ucgucucgaa accggaaagg     2340

aagaaaccca gcggggcccu ccacgaggag acuuuucgca aggaagagga guucuaucag     2400

uccuacggag guaaagaagg cgugcugaag gcacuagaac uggggaaaau ucggaaggua     2460

aauggaaaga ucguuaaaaa cggcgacaug uuuagagugg auauauucaa gcauaaaaag     2520

acaaauaaau uuuacgcugu cccuauuuau accauggacu ucgcccucaa ggugcugcca     2580

aacaaggcgg ucgccaggag caaaaagggu gagaucaaag auuggauucu uauggacgag     2640

aauuacgaau uuugcuucag uuuguauaag gauucucuga ucuuaauaca aacgaaagac     2700

augcaggagc ccgaacucgu guacuuuaac gcuuucacca gcuccacagu uucacugauc     2760

gugagcaagc acgacaauaa guuugagacu cuuucuaaaa accagaagau ucuguucaaa     2820

aaugcaaacg aaaaggaggu aaucgccaaa uccauuggga uccagaaucu aaaggucuuc     2880

gagaaguaua uagugagugc ucucggcgaa guuaccaaag ccgaguuucg ccaacgagaa     2940

gauuucaaga acgagcugcg guga                                            2964


<210>  2571
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2571
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag caauuuuaaa ggccaucaaa cagaaugaag agaagcuggc uaacuaucag      480

aguguuggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugagaaauaa aaaggagucu uacgaaaggu guauugccca auccuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa cagcgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguacu caguguggca uuuuauaagc gugcucugaa ggauuucagc      720

caucuugucg ggaacugcuc auuuuucacg gacgagaaac gcgccccgaa gaauagcccc      780

cuggcguuua uguucguggc cuugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa ucuuauacac uaaggaugac cucaauaccc uucugaacga gguuuugaaa      900

aauggcacac uaaccuauaa gcagacuaag aaacugcucg gccugucuga cgauuacgag      960

uuuaaggggg aaaaaggaac auauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcacuuggug agcacaaccu gagccaggac gauuugaaug aaaucgcuaa agacauuacc     1080

cucauaaagg augagaucaa acugaagaaa gccuuagcua aguaugaccu gaaccaaaau     1140

cagauugaua gucuaucuaa acucgaguuu aaggaccauc ugaacaucuc cuucaaggcc     1200

cuuaaauugg ugacgccucu gaugcucgaa ggcaagaaau acgaugaggc auguaaugaa     1260

cugaaccuua aggucgccau aaaugaggac aaaaaggauu uucugccagc uuucaacgag     1320

acuuacuaua aggacgaagu gaccaauccc guaguuuuga gggcgauuaa agaguaccgg     1380

aaggugcuca acgcacugcu gaaaaaguau gggaaagucc acaagaucaa uaucgaauua     1440

gcccgagagg ugggaaagaa ccacagccag cgcgcuaaaa uugaaaagga gcagaacgag     1500

aauuacaaag ccaagaaaga cgcagaacua gagugcgaaa agcucggccu gaagaucaac     1560

ucgaaaaaua uucuuaaguu gagacuguuc aaagagcaaa aggaauuuug ugccuauucu     1620

ggugagaaaa uaaagaucuc cgaucuccag gacgagaaaa ugcuggaaau ugaucauauc     1680

uacccuuauu caaggaguuu cgacgauagc uacaugaaca agguguuagu cuuuacaaag     1740

cagaaucaag agaaacugaa ccagacucca uucgaggcuu uugggaauga cuccgccaag     1800

uggcagaaaa ucgaaguucu ugcaaagaac uugcccacca aaaagcagaa gcggauucuc     1860

gauaaaaauu acaaggacaa agaacaaaag aacuucaaag aucgcaaucu gaacgacaca     1920

agauauauag cgaggcuagu gcugaauuac accaaggauu aucucgacuu ucugccucuu     1980

ucugaugacg agaacacgaa guugaaugac acucagaaag gaagcaaggu acacguggag     2040

gcuaaaucag gcaugcugac aagugccuua cgucauaccu ggggcuucuc cgcaaaggau     2100

cggaacaacc accuccacca ugccaucgac gcugucauua ucgccuacgc aaauaacagc     2160

auugugaagg cuuuuucuga uuucaaaaag gaacaggagu caaauagugc cgaacuguau     2220

gcgaaaaaga ucagcgagcu ugacuacaaa aacaagcgaa aauuuuucga gccauuuucc     2280

ggguucagac agaagguucu ggauaagaua gacgaaaucu ucgugucuaa accggagagg     2340

aagaaacccu cgggagccuu gcacgaagag acuuuucgca aggaagagga guucuaucaa     2400

agcuacggug gcaaagaagg gguccucaag gcucuggagc uaggaaaaau ucggaaggug     2460

aauggcaaga ucguuaaaaa cggugauaug uuuagaguag acauuuucaa gcauaaaaag     2520

acaaauaaau uuuacgcagu gccuaucuau accauggauu ucgcccugaa gguccuccca     2580

aacaaggcug uggccagguc caaaaagggg gaaauaaaag acuggauucu gauggaugag     2640

aauuacgaau uuugcuucuc acuuuauaag gacaguuuga uccugauuca gacgaaagau     2700

augcaggagc ccgaguuagu cuacuuuaac gcauucaccu cuagcacagu gucccucauc     2760

guuucaaagc acgacaauaa guuugaaacu cugagcaaaa accagaagau acuuuucaaa     2820

aaugcgaacg agaaggaagu gaucgccaaa ucuauuggca uccaaaaucu gaagguauuc     2880

gagaaguaua uugucuccgc ucuaggagaa gugaccaaag ccgaguuucg ccagcgagag     2940

gacuucaaga acgaacuccg guga                                            2964


<210>  2572
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2572
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag caauuuuaaa ggccaucaaa cagaaugaag agaagcuggc uaacuaucag      480

aguguuggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugagaaauaa aaaggagucu uacgaaaggu guauugccca aucuuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa cagcgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguacu caguguggca uuuuauaagc gugcucugaa ggauuucagc      720

caucuugucg ggaacugcag cuuuuucacg gacgagaaac gcgccccgaa aaauuccccc      780

uuagcguuua uguucguggc cuugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa uccuguacac uaaggaugac cucaaugcac uucugaacga gguuuugaaa      900

aauucaaccc uaacauauaa gcagaccaag aaacugcucg gccugagcga cgauuacgag      960

uuuaagggcg aaaaagggac uuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcucuuggag accacagucu gucucaggau gacuugaacg agaucgccaa agauauuaca     1080

cucauaaagg acgaaaucaa acugaagaag gcuuuagcca aguaugaucu gaaucaaaac     1140

cagauugacu cccuaagcaa acucgaguuu aaggaucauc ugaauaucuc guucaaggca     1200

cuuaaauuga uaaccccucu gaugcucgag gguaagaaau acgacgaagc cuguaacgag     1260

cugaaucuua agguggcuau uaacgaagau aaaaaggacu uucugccagc guucaaugag     1320

acguacuaua aggacgaggu cacuaacccc gugguauuga gggcaaucaa agaauaccgg     1380

aagguucuca augcccugcu gaaaaaguau ggcaaagugc acaagaucaa cauugaguua     1440

gcucgagaag ucgggaagaa uuacucucag cgcgccaaaa ucgagaagga acagaacgag     1500

aacuauaaag caaagaaaga ugccgagcua gaaugcgaga agcucggacu gaagauuaau     1560

uccaaaaaca uacuuaaguu gagacuguuc aaagaacaaa aggaguuuug ugcuuacuca     1620

ggcgaaaaga ucaaaauuag ugaccuccag gaugagaaaa ugcuggagau cgaccacauc     1680

uauccuuaca gcagguccuu cgaugacucu uacaugaaua agguguuagu guuuaccaag     1740

cagaaccaag aaaaacugaa uaagacacca uucgaggccu uugguaacga uagcgcaaaa     1800

uggcagaaga uugaaguccu ugcgaaaaau uugcccacua agaagcagaa acggauacuc     1860

gacaagaacu auaaagauaa ggagcagaaa gacuucaagg aucgcaaucu gaacgacacc     1920

agauacaucg cuaggcuagu ucugaauuau acaaaggauu accucgacuu ucugccucuu     1980

ucagacgaug aaaacaccaa auugaaugac acgcaaaagg ggaguaaagu gcauguagag     2040

gccaaguccg gaaugcugac uagcgcauua cgucacacau ggggcuucuc ugccaaagau     2100

cggaacaauc aucuccacca cgcuauugac gccgugauca uugcauaugc uaacaacuca     2160

aucgucaagg ccuuuaguga uuucaagaaa gagcaggaaa gcaauuccgc ggagcuguac     2220

gccaagaaaa uaucugaacu ugacuauaag aacaaacgaa aguuuuucga gccauuuucg     2280

ggcuucagac agaaggugcu ggauaaaauc gacgagauuu ucguuagcaa gccggaaagg     2340

aaaaagcccu ccggggcuuu gcaugaggaa accuuucgca aagaggaaga guucuaccag     2400

ucauacggag guaaggaggg cgugcucaaa gcacuggaac uagggaagau ccggaagguc     2460

aauggaaaaa uugugaagaa cggcgauaug uuuagaguag acaucuucaa acacaagaaa     2520

acuaauaagu uuuaugccgu uccuauauac acaauggauu ucgcucugaa ggugcuccca     2580

aacaaagccg ucgcaaggag uaagaaaggu gagauuaagg acuggauccu gauggaugaa     2640

aauuaugagu uuugcuucuc ucuuuacaaa gacagcuuga uucugaucca aaccaaggac     2700

augcaggaac ccgaguuagu guauuuuaac gcguucacgu ccucaaccgu cagccucaua     2760

gugucuaagc augauaauaa auuugagaca cuguccaaga accagaaaau ccuuuucaag     2820

aaugccaacg aaaaagaggu uauugcuaag aguaucggga uucagaaucu gaagguguuc     2880

gaaaaauaca ucguaagcgc ccuaggcgag gucacuaagg cagaauuucg ccaacgagag     2940

gacuucaaaa acgagcuccg guga                                            2964


<210>  2573
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2573
auggccagaa uccuggcuuu cgacauuggc auaagcucca ucgggugggc auuuucugag       60

aacgaugaac ucaaggacug cggagugagg auuuucacca aagccgagaa ucccaagaca      120

ggugaaucac uugcgcugcc ucggcgcuug gcucgaagug cccguaaaag auuagcaagg      180

cggaaggccc gccugaacca ccuaaaacau cucaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agagcuucga cgagucccuu gcaaaggccu acaaaggcuc ucugauuucg      300

ccauaugagu ugagauuuag ggcucucaau gaacugcugu caaagcaaga uuucgcccgg      360

gucauccucc acauagcgaa acgccgaggg uacgacgaua ucaagaacag cgacgauaaa      420

gagaagggag caauuuuaaa ggccaucaaa cagaaugaag agaagcuggc uaacuaucag      480

aguguuggcg aguaccuuua uaaagaauac uuucagaagu ucaaagaaaa uuccaaggaa      540

uuuacuaacg ugagaaauaa aaaggagucu uacgaaaggu guauugccca gagcuucuug      600

aaggacgagc ugaaacuaau cuuuaagaaa caacgggagu ucgguuuuuc cuucucaaag      660

aaauucgaag aggaaguacu caguguggca uuuuauaagc gugcucugaa ggauuucagc      720

caucuugucg ggaacugcuc auuuuucacg gacgagaaac gcgccccgaa aaauuccccc      780

uuagcguuua uguucguggc cuugaccaga auaauuaacc uccugaauaa ccugaaaaac      840

acagaaggaa uccuguacac uaaggaugac cucaaugcac uucugaacga gguuuugaaa      900

aauggcaccc uaacauauaa gcagaccaag aaacugcucg gccugucaga cgauuacgag      960

uuuaaggggg aaaaaggaac uuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcucuuggug accacagccu gagucaggau gacuugaacg agaucgccaa agauauuaca     1080

cucauaaagg acgaaaucaa acugaagaag gcuuuagcca aguaugaucu gaaucaaaac     1140

cagauugacu cucuauccaa acucgaguuu aaggaucauc ugaauaucag cuucaaggca     1200

cuuaaauuga uaaccccucu gaugcucgag ggcaagaaau acgacgaagc cuguaacgag     1260

cugaaucuua agguggcuau uaacgaagau aaaaaggacu uucugccagc guucaaugag     1320

acguacuaua aggacgaggu cacuaacccc gugguauuga gggcaaucaa agaauaccgg     1380

aagguucuca augcccugcu gaaaaaguau gggaaagugc acaagaucaa cauugaguua     1440

gcucgagaag ucggaaagaa uuacucgcag cgcgccaaaa ucgagaagga acagaacgag     1500

aacuauaaag caaagaaaga ugccgagcua gaaugcgaga agcucggccu gaagauuaau     1560

ucuaaaaaca uacuuaaguu gagacuguuc aaagaacaaa aggaguuuug ugcuuacucc     1620

ggugaaaaga ucaaaauuuc agaccuccag gaugagaaaa ugcuggagau cgaccacauc     1680

uauccuuaca guaggagcuu cgaugacucc uacaugaaua agguguuagu guuuaccaag     1740

cagaaccaag aaaaacugaa ucagacacca uucgaggccu uugggaacga uucugcaaag     1800

uggcagaaaa uugaaguccu ugcgaagaau uugcccacua aaaagcagaa gcggauacuc     1860

gacaaaaacu auaaggauaa agagcaaaag gacuucaaag aucgcaaucu gaacgacacc     1920

agauacaucg cuaggcuagu ucugaauuau acaaaggauu accucgacuu ucugccucuu     1980

agcgacgaug aaaacaccaa guugaaugac acgcagaaag gaucaaaggu gcauguagag     2040

gccaaaagug gcaugcugac uuccgcauua cgucacacau ggggcuucag cgccaaggau     2100

cggaacaauc aucuccacca cgcuauugac gccgugauca uugcauaugc uaacaacucu     2160

aucgucaaag ccuuuucaga uuucaagaag gagcaggaaa guaauagcgc ggagcuguac     2220

gccaaaaaga uauccgaacu ugacuauaaa aacaagcgaa aauuuuucga gccauuuucu     2280

ggguucagac agaaggugcu ggauaagauc gacgagauuu ucguuucgaa accggaaagg     2340

aagaaaccca gcggagcuuu gcaugaggaa accuuucgca aggaggaaga guucuaccaa     2400

uccuacggug gcaaagaggg ggugcucaag gcacuggaac uaggaaaaau ccggaagguc     2460

aauggcaaga uugugaaaaa cggugauaug uuuagaguag acaucuucaa gcacaaaaag     2520

acuaauaaau uuuaugccgu uccuauauac acaauggauu ucgcucugaa ggugcuccca     2580

aacaaggccg ucgcaagguc uaaaaagggg gagauuaaag acuggauccu gauggaugaa     2640

aauuaugagu uuugcuucag ucuuuacaag gacucuuuga uucugaucca gaccaaagac     2700

augcaggaac ccgaguuugu guauuacaac gcguucacga gcuccaccgu cucauuaaua     2760

gugagcaagc augauaauaa guuugagaca cucucuaaaa accagaagau ccuguucaaa     2820

aaugccaacg aaaaggaggu uauugcuaaa uccaucggca uucaaaaucu uaagguguuc     2880

gaaaaguaua ucguaagugc ccugggagag gucacuaaag cagaauuucg ccagcgagag     2940

gacuucaaga acgagcuacg guga                                            2964


<210>  2574
<211>  2979
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2574
augagaaucu ucagcgacgg caggaacccc aaggaugggu ccucucuggc cgugacccgg       60

cgcgcugcac gagccaugcg uagaaggcgg gaccgccucc uuaaaagaaa gacaaggaug      120

caggcgaaac uggucgagca cggauuuuuc ccugcugaug ccgguaagcg gaaagcauug      180

gaacaauuaa auccauacgc ccugcgcgcu aagggccuac aggaggcacu ccugccgggg      240

gaauuugccc gagcucuuuu ccauauuaac cagagaaggg gauuuaaguc aaaucggaaa      300

acugacaaga aagauaacga caguggcguu cugaagaaag ccauagguca guugcgucaa      360

cagauggcgg agcaggggag ccgcacggug ggagaauauc ucuggaccag acugcagcaa      420

ggccagggcg uaagggcacg guaccgagag aagcccuaua caacugagga agggaagaaa      480

cgcaucgaua aguccuacga ccuguauauu gauagagcca ugaucgagca ggaguucgac      540

gcucucuggg ccgcacaggc ugccuuuaau ccuaccuuau uccacgaagc ggccagggca      600

gaucugaaag acacacuuuu gcaucaacgg ccacugcgcc ccgugaagcc uggaagaugc      660

acccuacucc cagaggaaga gagggcuccc cuggcccuuc cuucuacuca gcguuuucgg      720

auucaccagg aagucaacca ucugcgauug cucgaugaga aucugagaga aguggcuuua      780

acacuggccc aaagggacgc aguugugacc gcccucgaga cgaaagcuaa acuuucguuc      840

gagcagaucc gcaaacuguu gaagcuauca gguagcgucc aguuuaaccu ggaagacgcg      900

aagcggacug agcucaaagg caaugcaacc agugccgcuc uggccagaaa ggaacuuuuc      960

ggggcagccu gguccggauu ugaugaggcu cugcaggacg aaauagugug gcaauuggua     1020

acagaggagg gcgaaggugc ccucaucgca uggcugcaga cucacaccgg gguugaugag     1080

gcgagggcuc aggccauugu ggacgucucu uuaccagaag gauacggcaa ccugagccgc     1140

aaagcacuag cccgaaucgu gccggcucuc cgggccgcag ugauuacaua ugauaaggcu     1200

guccaggccg cgggcuucga ccaccauucc caacuggggu ucgaguacga ugccucagaa     1260

guugaggacc uugugcaccc cgagaccgga gaaauccgua guguauuuaa acaguugccu     1320

uacuauggua aggcucugca gagacaugug gcauucggca gcgggaagcc agaggauccc     1380

gacgaaaaga gguacggaaa gauagccaau ccuacggucc acauuggccu caaccaggug     1440

cgcaugguug ugaaugcucu gauccggaga uaugguaggc caacugaggu cgugauugag     1500

cuugcccgag aucugaaaca aucucgcgaa cagaagguag aggcacagcg gagacaggcg     1560

gacaaccaaa ggcguaaugc ccgcauccgg agauccaucg cugaaguuuu ggggauuggc     1620

gaggaaaggg ugcgaggauc agacauacag aaauggaucu guugggagga gcucagcuuu     1680

gaugccgcag accggcgcug ccccuacagu ggcguccaga uuucugcugc caugcuguua     1740

uccgaugaag uggaggucga acacauccug ccguucagca agacacuaga cgauucgcuc     1800

aacaacagaa ccguggcaau gaggcaagcc aaucggauaa agcgaaacag aacuccuugg     1860

gacgcucgcg cggaguuuga agcccagggg uggucuuaug aggauauucu gcagagggca     1920

gagcguaugc cccuucggaa aagauacagg uucgcuccag acggauauga acgcugguug     1980

ggugaugaca aggauuuucu ggcccgagcc cucaaugaca caagauaccu gucccggguu     2040

gcugcagagu acuuaaggcu gguguguccu ggcacccgcg uaauccccgg gcagcuuacg     2100

gccuugcucc guggaaaauu cggccugaac gauguccuag gucuggacgg ggaaaagaau     2160

agaaacgacc aucggcacca ugcgguggau gcuugcguua ucggcgugac cgaccaagga     2220

cucaugcaga gguuugccac agcaucagcc caggcucgcg gcgaugggcu gacucgacuu     2280

gucgacggaa ugccaaugcc cuggccuacc uauagagauc acguggagcg ggcaguaagg     2340

cauauuuggg ugagucaccg cccagaccac gguuucgaag gcgccaugau ggaggagaca     2400

agcuacggga ucagaaaaga uggauccauu aagcagaggc ggaaggcgga cggcucugcu     2460

ggucgugaaa uaagcaauuu gauccgcauu caugaggcca cucaaccgcu gcgacacggg     2520

guuucagcag auggccagcc cuuagccuau aaaggauacg ucggcgggag uaacuauugu     2580

aucgaaauca ccgugaauga caagggaaaa ugggagggcg aggugauuuc cacauuuaga     2640

gcuuacggug ucguuagggc cgggggaaug ggccggcucc gcaacccuca ugaaggucag     2700

aauggcagaa agcugauaau gaggcuugug aucggggaua gcgugcggcu ggagguagac     2760

ggagcagaac gaaccaugag aauugucaaa aucucuggcu caaacgggca gauuuucaug     2820

gcuccaaucc acgaggccaa uguggaugcg cgcaacacug acaagcaaga cgccuuuacg     2880

uauacaagua aauacgcagg cagcuugcag aaggcuaaga ccaggcgggu uacuauaucc     2940

cccaucggag aagugcguga uccugguuuc aaaggguga                            2979


<210>  2575
<211>  2985
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2575
augacccugg gccucgaccu ugggacaaac agcaucggau gggcccugau ugauuucgac       60

gauaaucagc aacccagaaa cuugauagcu ugcgguucca ggaucuuuca ggaggcagug      120

gaagccaaga cuggcacgcc uaaaaaucac gcgcggcgca ccgcucgagc cgcacguaag      180

uuagucgcca gaaggaaaca gcggcgcaca aagcugcuaa accuccugau uagacauggg      240

cuucugccac aggacguugc ugagaggaau gcauugcucc uggauaacca agccaauaac      300

ccguaccagc ugcggaaacg cggacucgac gaaaaguuag agcccuucgc uuuuggccga      360

gcccuguauc accuugcgca uagaaggggu uuccagucua aucggaaggc agccucagau      420

gaagacggga aagugaagac ugcuaucagu accuugcgug ccgagaugca gggacgcaca      480

cugggcgcau uucuagcuga ucaaccuacc cagagaaggc gguacacuga ccgagccaug      540

uauguagagg aguucgaaca gcucuggagc aaacaggcgu ccuuuacaca agaacagcug      600

acccaggccc uuaagguggc aauucacaac ucuaucuucu uucaacgccc acugaaaucg      660

cagaagcauu uggucggcaa guguacguuc gagcccacua gaaaaagggc ugccaaggcu      720

auacuccuga cccagcgguu ucgcaucuua caggauguga aucaccuggc cguuaaaaac      780

ccuauuacaa gagaguucag gccacucacu caagacgaac guaauaaguu ggcagaggcc      840

cuggauaaac agaagucaau gaccugggga gcugugcgga aagucaucgg guggaacaag      900

aaggacagcu ugcacgaagg ugagacauuu aaucuagaag agggcaaaaa ggacaaacug      960

auugggaacc gaaccgcgug cgaucucaga ggagcacugg gcgacaggug ggaugcccuu     1020

acggcucagc agcaagacga ucuggugacu gacuugcuca caaucgauaa caccgacggu     1080

uuccugaagc gcaugaaaac ucauugggag uucgaugacg ccacagcaga aguaauagcc     1140

aagauugagu uagaacccgg guacgcucgg cugagugcca aggcaaugag agcgauccua     1200

ccuuaucucg agcagggaau gauuuacucc gaugcuugua aagccgcagg cuaugaccac     1260

ucuaauccaa accagaggac caucacggac aagcugggcc uuccgagcaa uguucgcaac     1320

cccguggucc agaaagccuu guuugaaacc cgaaaggugg ugaaugcuau cguccggaaa     1380

uacgggaagc cugccguuau ucguguggag auggcaagag auaugaagcu guccaaaagg     1440

caaaaggagg aacuccagaa agagcagaac aagcagaaaa aggcuaauga acuggccgag     1500

ggaauacuuc gcaaggaguu cgguauccaa cagccagcgc gggccgacau ucagaaauac     1560

aaccuguggu uggaaugcaa ggagguaugu cccuauacag gcacuaccau cucaagagaa     1620

augcucuuua guccugaggu ggaugucgaa cauauacugc cauacagcag gucuuuagac     1680

gauuccuaua ugaauaaaac acugugcaug gcuacuuuca accgacaagu gaagcuaaau     1740

aacacccccu acgagauugg gcagggagac ccgcucgcau augccgagau cuuucagcgc     1800

aucaaaacac ugccuuuccc caagcggaga agguuugaac agaaagagau ugaaaccgau     1860

aaguucaucg agcgucaacu uaaugacacu cgcuacauuu guguugaagu gcgggauuuu     1920

cuucagcagc ugggcgcucc agucgaggug ucaaagggug aggccacggc aaaacucaga     1980

gggaggugga agcugaacac auuacugagc ccugacggca guaaugaaaa auaucgagga     2040

gaucaccggc accaugcgau agacgccgua guuaucgcuc uuaccaaccg cucuuuguuc     2100

cacaagcucu ccagacugag cgcccagucg auuucucuag auaaauccag guuugacgug     2160

cccccacccu gggacggcuu cuacgaugac gucuacgaga agaucgauaa ggugaucguc     2220

ucacaugcuc cugcacggaa aauuggggga gcccugcacg aagacacugc auauggucau     2280

uuuaaggagg aaguggccga uccaaaaacc ggcaagguua guauagagga ccgauucgug     2340

uacagaaaac cgcucgcuac acugaccgcg aacgagguag ggaagauccg cgaugccaag     2400

gucagggagu ucguggaagc acgucuugcu caauuuggaa auaacagcaa aacugccuuc     2460

ggcgacccca auaacccuuu gcugcacgcc gaugguaaga caccaauucg gacgguuaga     2520

uuagugacca auuuuaacaa agcuacuaca cacgcaaucc agaaggggca gggcagggac     2580

uauaaauucu uuaaguacgg aaauaaccau cacgucgaaa uuaucgagca uguggaaacc     2640

ggcaagcgca aagggauauu cguaacagcc auggaggcgg cucgaagagu gcggggaguu     2700

gccaauacug agaaguccga uaucguccag aggaaccacg gugaagagug gcgcuuugug     2760

augucucuca gcauuaauga ccugguguca auccaagaag cagaucaggu ccguauuaug     2820

agaguucagc uuuucgacgc caguaacggc aauaucauau uucgggacca aaaauccgcu     2880

augcuggaug acaaguugag cagguaucag accaaagcac accuccuggu gugccagaag     2940

auuacgaccg auccccuagg gaaccuggcc ccuuguaaug acuga                     2985


<210>  2576
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2576
augagcaagg ugcugggccu cgacaucggg gccaacucca uuggaugggc ucuuauagau       60

gacgaggaaa augauaucau uggugcaggc gucagaguuu ucaaagaggg ggugaaccug      120

aagggaguaa aagaagaguc uaagaaugug gaaaggcggg ccgcgcgcuu ggcucgacgu      180

cagcacaaaa gaaggucaca acggcgcaac agauuagucg cccugcuaaa gacccucgag      240

auguuucccg agaauaacga agacaucagu gauuacuaug cacaggaccc uuacgagcug      300

aggaagaaag gccuuuauga aaagcugaca auguacgagu ucggucgggu guugcuccau      360

auuugccgcc gaagaggguu uaaaagcucc aggaagacug auaucucuga ggaaggaaaa      420

auauucgagg gcaaggacgg caucguuggg auuaaucaga cgcgggauuc gauccgugaa      480

ggagguuuua agaccauugg cgaguaucug gccucacugg acccacacgu gauccgcaga      540

aggaaccggu ucacacuccg agauauguac gaagaggagu uuaauuuaau gugggaaaaa      600

cagcagagcu ucuauccgcu gcaacuuagu aacgaguuua aggaagcuau agcagagguc      660

auuuuguccc agcgcaaacu gaagucucag aaagcccuag uggggaauug uggacucgaa      720

cccaacaaga aaacugcucc uaagagcucc ccagccuucc aauacuuuag aauccuggag      780

cagcuuucaa ggauucugau caguaucccc cauaccggcc gguacaauga cccuuugaac      840

gaggaggagc gcagaaugcu cauugauaag cugagcguaa cagaaaaauu aaaguucucu      900

gacaugagga aaaugcugaa ucucccaggu gaguccgaca uaaacaucga agaggaaggg      960

ggacgucuuc ugggcaacac cacuuaugcg cgguugauua agguuuuugg caaaaagcga     1020

ugggcagggu ucucagaaga ggaaaaggag cagaucuggc acacauucca uuuugccgau     1080

aaucacgagu ggcuaaaaga auacgcucug aagaaaugga gcauaacccc cgacgagauu     1140

aaggaacucg gaaaaguggc ccuggagaag gaauaugcaa gauucaguaa gaaaucucuu     1200

aagaaaauca ucccucagcu ggaggagggu uugacgcucg auaaggcuaa aauugaagcc     1260

ggcuacacuc cagaguauga aaucgagaag ucccugagcg cguacuuacc gcugcccgaa     1320

aacauuagga auccuauagu ccaacaggcc cuauaugagc uccgccacgu ggugaacgca     1380

gucauccgga ccuacgggaa gccagacauu gcuagaguug agcuggccag ggaaaugaaa     1440

aagcccaaac agaagcgcga gaaaaugcga ucggaacuuc ggaagcguca aaugaaauuu     1500

gaggaaauca agucuaucuu gaaggagaga cugggauuca uugauccuaa aagggacgag     1560

auacauaagu acaugcucug ggaagagugc cgcgaaacau guccauauac uggccggaaa     1620

aucucccugu cagcucuuua cgguggggau uuugaggugg agcacauuau ccccuauaag     1680

agaagucugg acgauagcuu guucaauaaa accguaugcc ucagggaaga gaaccagauu     1740

aagggcgacc gaacaccgua cgaagccuau uccggagauc gccagaagua cgaggaaauc     1800

auacugcggg ugcagaaaac cucuaugccu agaggcaaga aaaauuuauu ugcaaucaag     1860

gagcugaaag acgacuucgc ccauaggcaa cuaagcgaua cggcuuauau uucacgugag     1920

acucgcaagu accucgggag uacaaucaag aaaguccagg cggugaaggg agguuccacc     1980

gcaaaacugc gguacuauug gggccuuaac agcauuuugg ggguuagaga caucaagaug     2040

agggaugacc accgacacca ugccguggau gcucugguca uagccaauac uacauacgca     2100

uuugugaaca cccucucucg guaucacuca auggacguag aaccccauga gagucgcuuc     2160

ccacugccuu gggaacaguu uagaaaugau gccgagagcg uugugaggaa cauuuucguc     2220

ucccaccggu uaggcaaaaa ggcuacggga cagcugcaug aagagaccua cuaugguaag     2280

auccgaaaua cagacgggga gcccuacuuu guggucagaa agaaacuugg cacuuugacc     2340

gccaaacaaa uugcaaacau cguggaucca guugugaaga cacagguauu cgaacgccuc     2400

gaggaaaaag gaauagacau cgagucuucg aauaagauuc ccgcggaugc uuucgucgaa     2460

acucuguaua ugccugacag gaaaacccca aucaagagcg ugcguguuaa ggugccgucc     2520

acaaccauga uucagcuaua caaagauaag gagcuguacg ucgaccccgg cucaaaccac     2580

cacaucguga uauuugagaa cgaagagggg aaacgggacg gaauuguagu gagucucuuc     2640

gaagccgcac agagacugag gcgcaaugag ccuguuaucc aaaagacuug gaaaccagag     2700

uggcauuuug ucaugucucu uagcaucgau gaauugauuc uguuagacgu ggaugagaag     2760

aacaucgacu ggggugauac gggccucgcc cugcgauauu ccaaggcucu uuacagagug     2820

cagaaaaugg acgucaauaa gauuauaaca cugcggcacc auuugguugc caggcucaaa     2880

gaugacgaag ggaaggaguu cggacgcgaa caccguaucc ccucaacccu gagaggccua     2940

aaagugaaga uugaucacau cggucggcug aggcgcgcau augacuga                  2988


<210>  2577
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2577
augaagguga ucggcuucga cauugggaua aacagcaucg gaugggccuu ugucgagaau       60

gaugaacuga aagacugcgg uguuagaauu uucaccaagg cugagaaccc caaaaauaag      120

gaaucccucg cacuuccuag gcggaacgcc aggucuucac gaaaacgucu gaagagaagg      180

aaguugcggu uaaucgcgau gaaacacauu cuggcuaagg agcuaaaucu caacuacaaa      240

gauuauaucg ccaaugacgg cgaacugcca aaggcauacg aggggaaacu uauaaguccg      300

uaugagcugc gcuacaaggc cuugaaccag aagcucgaag cuaaagaucu ggugagagua      360

auccugcaua uugcaaagca caggggauau augaauaaaa acgacaagaa aagcaaugau      420

gccgagaagg gcaaaauccu cuccgcuuua aagaacaaug cccugaaacu ugaaaaauac      480

caaucugugg gugaguauuu uuacaaggag uucuuucaga aauacaagga aaacacaaaa      540

gacuucauua agauccggaa uauaaaggau aacuauaaca auugugucuu ggcgucggac      600

cuggagaaag aacuaaagcu cauucuggag aaacagaagg aauuugggua cucauauagc      660

aacgauuuca ucgaggaggu gauucagguu gcauuuuucc aacgcccccu uaaagacuuu      720

agucaucugg ugggaacuug cacguucuuu gaagaggaaa agcgagccug uaaaaauucc      780

uacucugcuu gggaguucgu cgccuugacc aaaaucguga acgaacucaa gagccuggag      840

aaaaucuccg gcgaguuaau uucaacacag acuauaaaug aaauccugaa cuauauucuc      900

gauaagggca guaucaccua caaaaaguuc agaagcuaua uaaaucuuua cgagucuauu      960

aaguuuaaau cccugaagua cgacaaagac aacgcagaau cagcuaaguu gaucgauuuc     1020

aggaagcuag uagaguuuaa gaaagcccug ggggaacacu auuucaaucg gcaggagcuc     1080

gaccagauca gcacauacau uacccugauc aaggauaacg uuaaacuuaa aacugcgcug     1140

gagaaguaua auuugaguaa cgaacaaauu aauaaccucc uggagauaga auuuaaugac     1200

cauaucaacu uaucuuucaa agcccugucc augauucuac cucucaugaa ggagggaaag     1260

aaguacgaug aggcaugcga aaucgcuaac cugaagccaa aaacaaccaa ucagaagcag     1320

gacuuucuuc ccgccuucug uaacagcauc uuugugcacg aguugucgaa uccuauuguc     1380

aaccgugcua uaucugaaua ucgcaaagug cugaaugccc uccugaagaa auacgguaag     1440

gugcacaaga uccaucuuga gcuggcaaga gauauuggcu uguccaaaaa ggccagggaa     1500

aagaucgaga aggagcagaa agaaaaccaa gcuauuaaua cgugggcgcu caacgagugc     1560

gaaaauaugg ggaucaaggc aaacgccaag aauauacuga aauuaaagcu guggaaagag     1620

cagaaggaaa ucuguauuua uucaggcaaa aagaucagua uugagcaccu aaaagacgag     1680

aaggcucucg aagucgauca uaucuaccca uacagccggu ccuucgacga uucuuauaug     1740

aacaagguuc ugguguuuac uaaagagaau caggaaaaac uuaacaaaac ccccuucgag     1800

gccuuuggaa agaauauaga aaaauggaac aagauucaaa cauuggcaca gauccugccu     1860

uacaagaaaa agaacaaaau ucucgacgag aauuucaagg acaaccagca gcaagauuuc     1920

aucagccgaa aucugaacga cacucgcuau auagccaccu uaaucgcuaa auacacaaag     1980

gaguaucuga auuuucuucc auugaacgaa aaugagaaca uuaaucucaa gucaggugaa     2040

aaagggagua agauccacau ucagaccgua aacggaaugc ugacguccgu gcuaagacac     2100

acuuggggcu ucgauaaaaa ggacaggaau aaccaucugc accaugcccu cgaugcaauc     2160

auagucgcgu acagcacaaa uucucugauu aaggcuuuuu cagacuucaa gaagaaccag     2220

gagcuuuuga aagccaagcu guaugcaaaa gaguuaacca augauaacua cauccaccag     2280

gccaaguuuu ucgaaccguu ugagggcuuc cgggagaaaa uccucaacaa gauugacgaa     2340

aucuuuguga guaagccccc ucgcaaaaga gcucgcgggg cccugcauaa ggaaacuuuc     2400

uacagcgaga aaaagaugau uaaaaaguau aauuccaaag agggacuuaa cauagcacug     2460

gauugcggua agauccguaa gauuggcaca aaauacguug aaaaugacac cauggugcgg     2520

gucgauaucu uuaagaaaca aaacaaguuc uaugcuauuc caaucuacac gauggacuuu     2580

gccuugggga uacuccccaa uaaaaucgug guaauuggaa aggauaagaa caauaacccu     2640

aaacaguggc aggagaucga cgaaucuuau gaguucuguu ucucgcugua caagaaugac     2700

cuaauucugc uccagaaaaa ggauaugcaa gaagcggagu uugccuauua caacggcuuc     2760

gacaucgaua gcucccgaau aaauauuaaa aagcacgaca acaaguuuga gaaucugacc     2820

ucaaaccaga aacuuuuguu cacaaauaag gagaaaaaca gaacugguau ccagaaucug     2880

aagaucuuug aaaaauacau uguuacccca uuagggaaca agauaaaggc ugauuuccag     2940

cccaggcaaa acaucagucu caaaucuagc aagaaaaaug gccuguga                  2988


<210>  2578
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2578
augaagaucc ugggcuucga cauugggaua aacagcaucg gaugggccuu uguggagaau       60

gaugaacuca aagacugcgg ugucagaauu uucaccaagg cugagaaccc caaaaauaag      120

gaaucccuug cacugccuag gcggaacgcc cgcucuaauc gacguagauu gaaaaggcgg      180

aagucacgcu uaaucgcgau uaagcacauc cuggcuaaag agcuaaaccu caauuacaag      240

gauuauauag ccaacgacgg cgaacugcca aaagcauacg aggggaaguu aacaaauguu      300

uaugagcuga gauacaaagc cuugaaccag aagcucgaag cuaaggaucu ggcaagggug      360

auccugcaua uugccaaaca ccggggauau augaauaaga acgagaaaaa gaguaaugac      420

agcgaaaaag gcaagauccu cuccgcuuua aagaacaaug cccugaaacu ugagaaguac      480

caaucuguag gugaguauuu uuacaaagag uucuuucaga aguacaaaga aaacacuaag      540

aacuucauua aaauccgcaa uacgaaggau aacuauaaua acuguguguu ggcgucggac      600

cuggaaaagg agcuaaaacu cauacuggaa aagcagaaag aguuugggua cucauauaau      660

gaugacuuca uuaaggagau cauuaacauc gcauuuuucc agcgaccgcu uaaagauuuu      720

agccaucugg ucggaaguug caccuucuuu gaagaggaaa agagagccug uaaaaauucc      780

uacucugcuu gggaguucgu ggccuugagc aagaucauua acgaacucaa gaggcuggag      840

aaaauauccg gcgaguuaau cucagcacaa acaauuaaug aaauccugaa ccacauacuc      900

gacaagggca auauuacuua uaaaaaguuc cggaguugca ucaaccuuca ugagagcauc      960

gaauuuaagu cucugaauua cgauaaagac aacgcugagu ccgccaaguu gguugacuuc     1020

aaaaagcuaa uugaauuuaa aaaggcgcug ggggagcacu cauucaaucg ucaggagcuc     1080

gaugaaaucg ccacccacau uacacugaua aaagacaacg auaaacuuaa gaccgcacug     1140

gagaaguaua acuugaauaa cgaacagauc aaugcucucc uggagauuga guuuaacgac     1200

cauaucaauc uuagcuucaa ggcccugaac cuaauccucc cccugaugcg cgaaggaaaa     1260

agauacgaug aggcuuguga aauugugaau cuuaagagua aaacuucuaa ccagaagcaa     1320

gacuugcugc cugccuuuug cgauuccaua uucagccagg agcucucgaa uccagucgug     1380

aacagggcaa ucucugaaua ccggaaggua cugaaugccc uucugaaaaa guaugguaaa     1440

guucacaaga uucauuugga gcucgcucga gacgugggcc uguccaaaaa ggcgcgcgag     1500

aagaucgaaa aagagcagaa ggaaaacaaa gagauuaaug auugggcauu agacgaaugu     1560

gagaaguuug ggcugaaagc caacaauaag aacauccuaa agcucaaacu guggaaggag     1620

cagaaagaaa uaugcgcuua cucaggaaag aaaaucagua uugagcaccu uaaggaugaa     1680

aaagccuugg aggucgacca caucuauccc uacagcagau ccuucgacga uucuuauaug     1740

aacaaggugc ugguguuuac aaaggaaaau caagagaaac ucaacaagac cccuuucgag     1800

gcauuuggca aaaauauuga aagguggaac aagauccagg cccuggcuca gaauuuacca     1860

uacaaaaaga agaacaaaau acuggacgag aauuucaagg auaaacaaca gcaggacuuc     1920

auuagccgga accuuaauga uacgcgcuau aucgccacuu ugauugcaaa guacaccaaa     1980

gaauaccuca acuuucugcc ccuaaaugag aacgagaaua caaaccugaa gucaggugaa     2040

aaggggagua aaauccaugu ccagacuaua aauggaaugc ucaccuccgu ucugagacac     2100

acauggggcu ucgacaagaa agauaggaac aaccaucuuc accaugcguu ggacgcuauc     2160

auuguggccu auagcaccaa uucuaucauu aaggcauuuu cagauuucaa aaagaaccaa     2220

gagcuguuaa aggccaaacu cuacgcuaag gaacugacga gugacaauua uaaacaccag     2280

gccaaguuuu ucgagccuuu ugaaggcuuc cgugagaaaa uccuuaacaa gauagaugag     2340

auuuuuguaa gcaagccacc gcggaaacga gcaagaggga cucugcacaa ggaaacauuc     2400

uaccccgagg aaaccaugau caaaaaguau aauuccaaag agggauugaa caucgcucuc     2460

aauuguggua agauuaggaa aaucggcacu aaguacgugg aaaacgacac aaugguccgc     2520

cuggauauuu uuaagaaaca gaauaaguuc uaugccauac cuaucuacac cauggacuuu     2580

gcgcuaggga uucugccaaa caaaaucgug auugccggaa aggacaaaaa uaacaauccc     2640

aagcaguggc aagagaucga ugagucuuac gaguucugcu ucucgcucua uaagaacgac     2700

cugauacuuu ugcagaaaaa ggauaugcag gaagcugaau uugcauacua uaauagcuuc     2760

gacaucgauu ccucacggau uaacaucaaa aagcaugaca auaaauuuga gaaccugacg     2820

gauaaccaga aguuacucuu caccaaucaa gaaaagaaca gaacaggcau ucagaaucug     2880

aaaaucuuug agaaguacau aguuacuccu cuuggugaca aaauuaaggc cgauuuccag     2940

ccaaggcaga acaucagucu gaaaaccucu aagaaguaug ggcuauga                  2988


<210>  2579
<211>  2991
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2579
augaugggca acuucacccu ggggcucgac cuuggaacaa auagcaucgg uugggcccug       60

auugauuuug acgauaacca gcaaccccag aauuugauag cuugcggcuc cagaaucuuc      120

caggaggcag uggaagccaa gacugggacg ccuaaaaacc acgcgaggcg ggcugcccgc      180

gcagcccgaa aguuagucgc ucguagaaaa cagaggcgga ccaagcugcu aaaucuccug      240

guuugucaug accuucugcc acaagaugag gcagaacgca acgccuugcu ccuggacaau      300

caggcuaaca auccguacca gcugagaaaa aggggacucg augagaaguu agaacccuuu      360

gccuucggcc gggcgcugua ucaccuugca caucgccgag guuuucaguc uaacagaaag      420

gccgcuucag acgaggaugg gaaagugaag acagccauua gugcauugag ggcugagaug      480

caaggacaca cucugggcgc cuuccuagcg gaccagccua cccagcggcg ucgcuacaca      540

gauagagcca uguaugcaga agaguuugaa cagcucugga gcaaacaaac caucuucgcu      600

ccagagcagc ugacucaggc ccuuaaggua gagauuaaaa auuccaucuu uuuccaaagg      660

ccccugaagu cucagaagca uuuggugggc aaaugcacau uugaaccuac ccggaagcga      720

gcugccaaag caauacuccu ggcccagcac uuccgcaucu uacaggacgu caaccaccug      780

gcugugaaga auccaauuac gagagaguuu aggccccuca cucaagauga acggaacaaa      840

cuugcggagg cacuggacaa gcagaaaggg augaauuggu cggccguucg caaggugauc      900

ggauggaaca agaaagacuc auugcaugaa ggugagaccu ucaaccuaga ggaaggcaag      960

aaagauaagc ugauugggaa uagaacagcu ugugaccuca ggggagcccu gggcgaucgu     1020

ugggacgcau ucacugcccc ucagcaggau gaccuuguca ccgaucuguu gacaaucgac     1080

aacccagaug guuuucucaa acggaugaag acccacuggg gguucgacga ugcuacggcc     1140

gaggcacugg cgacuacaga auuagagccg ggauacgcuc gacugagcag uaaggccaug     1200

agagcaauac uaccccaucu cgaacaaggc augauuuauu ccgacgccug caaagcugcc     1260

ggcuacgacc acucuaaucc uaaccagagg accaucacug auaagcuggg gcuuccaagc     1320

aaugugcgca accccguagu ucagaaagca uuguuugaga cacggaaggu ggucaaugcu     1380

auugugagaa aauauggaaa gccugccgug aucagggucg agauggcgcg cgacaugaag     1440

cuguccaaac gacagaagga ggaacuccaa aaagaacaga acaagcagaa aaaggccaau     1500

gagcuggcug aagguauccu ucggaaggag uucggcauuc agcaaccagc acguaccgau     1560

auacagaaau acaaccugug guuggagugu aaggaaguuu gccccuacac ggggaccgug     1620

aucucaagag agaugcucua uaguccggaa guagacgugg agcacauucu gccuuacagc     1680

aggucuuuag augacuccua uaugaauaaa acacugugua uggccacuua caaccgccag     1740

accaagcuaa auaacacacc cuaugagauc ggacaaggcg auccagucgc uuacgccgaa     1800

auauuucagc ggauuaaaac ucucccuuuc cccaagagaa ggcgauuuga gcagaaagaa     1860

aucgagaccg acaaguucau cgaacgccag cugaaugaua cacgguauau uugcguggag     1920

guuagagacu uucuucaaca guugggugca ccaguggagg ucucaaaggg ggaagcgacc     1980

gccaaacuga ggggccguug gaagcucaac acucuguuaa gccccggagg cgggaacgag     2040

aaaaaucgcg cugaucaucg gcaccaugcc aucgacgcag ugguaauugc ucugacgaac     2100

agaagucuuu uccacaaguu gucuaggcuc uccgcccaga gcauaucguu ugauaaaucu     2160

cgauucgacg uuccuccacc gugggacgau uuuuacgacg auguguacga aaagaucgac     2220

aaggucauug ugucccaugc acccacacgg aaaaucucag gagcccugca cgaggcuacc     2280

gcguaugguc acuucaagga agagaucgcc gauccuaaaa cuggcaaguu caguauugag     2340

gaccgcuuug ucuacagaaa accacuaacc acacugaccg caaaugaagu ggggaagaua     2400

agggaugcua agguucggga gcucguggaa gcccgacugg cccaguucgg aaacaauagc     2460

aaagcugcau uuggcgaccc caacgagccu cuuuugcaug ccgaugguaa gacuccaauc     2520

agaacaauuc gccugacgac caauuucaac aaaacuacaa cccacgcgau ccaaaagggg     2580

caggacaggg auuauaaauu uuucaaguac ggcaauaacc aucacguaga aauuaucgag     2640

cacgucgaga caggaaagcg uaaaggcaua uuugugacug cuauggaagc cgcacggaga     2700

gcccgcgggg uugcuaauac cgagaagucc aacaucgugc agaggaauca uggagacggu     2760

uggaaauucg ucaugucuuu agcagugaac gauaugguac gaacggaaga cacccaggcc     2820

auuuauagag ugcaaaagcu cgacggcagc aauaaccggu uuacacugag gucacacaac     2880

gcggcuacuc uugaugacaa acagaccggg guugauaaga cacccaaucu guucaaagga     2940

acuaagauca uugucgaccc guugggcaac cucacccagu guaaugauug a              2991


<210>  2580
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2580
augaaggugc uggucgacgc cggccucaug cccagcgauc cugcugcagc caaagcgcuu       60

gagacccugg acccauacga auugagagcu acaggguuag auguuaugcu gccgcuaacu      120

caccucggaa gggccguguu ccaucugaac cagcggcgcg guuuuaaguc caaucgaaaa      180

acggaccgug gcgauaacga gucugggaag aucaaagacg caaccaagag acuuacaugg      240

gccaugcaag aagcuggagc aaggacuuau ggcgaguucc ugcacaugcg gcgcgccaga      300

gcuucagauc ccaggcaggu accuagugug cggacccgcu ugagcgucgc cucccgaggu      360

gggccagacu cuaaggaaga ggcgggauac gauuuuuauc ccgagagagc acaucucgaa      420

gacgaguucc acgcccugug gucggcucag gccgcauuuc auccugaucu gacaugcgaa      480

cucagggacg ugguuuucga gaaaauauuu uaccagcggc cauuaaagga gcccaaagug      540

ggccuguguc uuuucaccgc ugaggaacgu uugccuaagg cccacccacu gacuggccgc      600

agaguccuau augaaacagu gaaucaacuc aggguaaccg cggaugggcg gacgacucga      660

ccgcugaccc uugaggaacg cgacaugauu guuuacgccc uggacaacaa aaagcccgug      720

aagucauuga gcaguaugca ccucaaacug gucgcauuag gaaagcuggu gaaacucaag      780

gauggugaga gauuuacacu ugaguccggc gugagggacg cuaucgccug cgauccuauu      840

cgggcuacua uggggcaucc agacggauuc ggccccgccu ggucucgccu ggauuggcag      900

gcacaguggg ccuugaucga aaaacuaaga cagguccaaa gcgacgauga cuuugcuucc      960

cugguugcgu ggcucggugc aacccacaau cugucagagg aucaugccaa gaggguggcu     1020

aaccuucauc ugccugaagg guauggacgu uugggccuca cagccaccga ggcaauucug     1080

uguaauuuaa aagccgaagu augcacguac agucaggcug uggagcggug gggcaagcac     1140

cacagcaacc agcgaacugg ggagugucug gaagcccuac cauacuaugg agagguccuc     1200

gacagacacg ugauccccgg uacauacaau gaagaugacg acgauguuac cagguauggc     1260

cgcauaacua acccgacagu gcauaucggg cugaaucagc uucggagauu ggucaacagg     1320

auuaucgcaa ccuacggaaa gccugaccaa auugugguag agcuggcgcg cgaacucaaa     1380

cagucugagg cucagaaguc cgaggcccug aaaaagaucc gagauacgac cgcagacgcc     1440

auacggcgug gcagacaacu ugaagagauu ggucagaaaa auacaggggc uaacaggcug     1500

uugcuccgcc ugugggaaga uuuaggcccc gccguuggac cacggugcug uccuuauacu     1560

ggcaagccca ucucagugac caugcuguuc gacggggcau gcgaugucga ccacauucua     1620

ccauacagca gaacacucga ugacaguuuu gcuaauagga cuaucugucu gcgagaggcc     1680

aaccgccaga agggagaucg gacccccugg gaggcguggg guggcucuga ccagugggau     1740

gccaucgaag cuaaccuuaa agacuugccu gagaauaaga gaaaaagguu cgcaccagac     1800

gccauggaac guuucgaggg ggaacgcgau uuucuggacc gggcucucgu ggauacacaa     1860

uaucuggcca gaauuuccag ggcauacuua gacacccugu ucacugaggg aggccauguc     1920

ugggugguuc cgggucgaau gacggagaug cuucggcgcc acuggggguu gaacgcgcuc     1980

cugagcgaua aggacagagg cgccggaaaa gugaagaaua ggacagauca ccggcaucac     2040

gcuauagacg ccgcaguaau cgcugccacc gaucauucgc uagucaaccg aauuucuaag     2100

gcagccggcc agggggaauc cgcuggacag ucagcggagc ugaucgccag agacacuccc     2160

gaaccuuggg aggguuuucg caaugauaua agugcacaga uugcuaggau cgugguuagc     2220

caccgugccg accacggccg gaucgaugug gaagggagaa aacauggaaa ggacuccacc     2280

gccggcaggc uccacaacga cacagcuuac gguauugucg augaccauac cgugguaucu     2340

cgcacuccac ugcuuagcuu gaaacccggg gauaucgagg ugacaacgaa gggcaaaaau     2400

auucgagaca cccaacugca gaaggcauua ucaguugcca cuacaggaaa ggauggcaaa     2460

ggguucgagg aagcgcucag aaacuuugcu gagaaggaag gaccuuauca gggcauacgg     2520

aggguccgcc ugaucgagac ccuucagagu uccgcccgug uggaaguggg uagcgacgag     2580

gaugggcaac cacugaaagc auacaaggga gacucuaauc acugcuauga uuuguggaaa     2640

cuccccgacg gcaaggucgu uccgcaugug gugucaacau acgaggccca cgcugguacu     2700

gauaaaagac cucacccagc agccaagcgg auucugaggg uauucaagaa agacaugguc     2760

gcgaucgaac aggacgauca gaccgcuauc uuuuaugugc agaagcuaga ccgcgccaac     2820

ggccuguucc ucgcacccca ucuggaggcc aaugcugaug cccgacacag aaacccuaau     2880

gacgcauuua aauuccuuca aaugggggcu ggaaguguug ugcggaacaa guugaggcgc     2940

gucuacgugg augaaauugg cagaauaaaa gaccccgggc caccuaagca uuga           2994


<210>  2581
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2581
augaagauac ugggcuucga cauugggaua aacagcaucg gaugggccuu uguggagaau       60

gaugaacuca aagacugcgg ugucagaauu uucaccgagg cugaaaaccc cuccaauaag      120

gagucucuug cacugccuag gcggaacgcc cgcucaaguc gacguagauu gaaaaggcgg      180

aaggcgcgcu uaguugcuau caaaaggauu cuggccaagg aacuaaagcu caauuuuaaa      240

gauuacgugg cagccgacgg cgagcugcca aaggcuuaug aggggaaacu uacaagcauc      300

uacgaacuga gauauaaggc auugauacag caacucgaga cuaaagaucu ggcccgggua      360

auccugcaca uugcuaagca ucgcggauac augaacaaga augagaaaaa guccaacgac      420

acgaaaaagg gcaaaauccu cucugccuua aagaauaacg cgcugaaacu ugaaaauuau      480

cagucggugg gugaguacuu cuauaaggaa uuuuuccaga aguacaaaga gaacaccaag      540

aauuuuauua aaauccgaaa cacaaaggau aauuacaaca acugugucuu gucaagcgac      600

cuggaaaaag agcuaaagcu cauacuggaa aagcagaaag aguucgggua uaauuacagu      660

gaggauuuua uuaacgaaau ccuuaaggug gcauucuuuc aaagaccgcu gaaagacuuc      720

ucccacuugg uuggagccug caccuuuuuc gaggaagaga agagggcuug uaaaaauucu      780

uauagcgccu gggaguucgu ggcacucacc aagauuauca acgaaaucaa gucccuggag      840

aaaauuucag gcgaaauagu ccccacacag accaucaaug agauuuuaaa ccugauccuc      900

gauaagggca guauaacuua caaaaaguuu cggagcugca uuaaucuuca ugaaucuauc      960

uccuucaaau cacugaagua ugacaaggag aacgcugaaa augccaaauu gaucgauuuu     1020

cguaagcuag uggaguucaa aaaggcgcug gggguacaca gccucagucg ccaggagcug     1080

gaccagauuu cuacacauau cacccuuauu aaagacaacg uuaagcugaa aacgguguug     1140

gaaaaguaca aucucuccaa cgagcaaaua aauaaccugu uagaaaucga guuuaaugau     1200

uauauuaacc ugagcuucaa ggcccuagga augauccucc cucugaugag agaggguaaa     1260

agguacuacg aagcauguga gaucacuaac cuuaagccaa aaaccgucga cgaaaaggag     1320

gacuuuuugc ccgcuuucug cgauucgauu uuugcccacg aacugucuaa uccuguggug     1380

aaccgggcua uauccgagua ucgaaaaguc cucaaugccc ugcuuaagaa guacggcaaa     1440

guucacaaga uccaucugga guuggcacgc gauguggggc ucaguaaaaa ggccagagag     1500

aaaauugaag accagcagaa gaacaauaag gcuaucaacg auugggcgcu gaaagaaugu     1560

gagaauauug gaaucaaggc aucagccaaa aacauauuaa agcugaaacu auggaaggaa     1620

cagaaggaga ucugcauuua uagcggcaau aaaaucucca uugagcaccu caaggacgaa     1680

aaagcucugg agguagauca uaucuaccca uauucuagga gcuucgacga uucauuuaua     1740

aacaaggugc uugucuucac aaaagaaaau caagagaagu ugaacaaaac ucccuuugaa     1800

gccuucggua agaauauuga gaaguggagu aaaauccaga cccuggcaca gaaccucccu     1860

uacaagaaaa agaauaaaau ucuggacgag aacuucaagg acaagcaaca gcaggauuuu     1920

aucucccgga acuuaaauga cacacgcuau auaaccacgc ugaucgccaa auacacuaag     1980

gaauaccuua acuucuugcc acucagcgag aaugaaaaca caaaucugaa aucuggggag     2040

aagggaucaa aaauucacau ccagaccauu aguggcaugc uaacuagcgu gcugagacac     2100

acauggggcu uugauaagaa ggacaggaac aaucaucucc accaugcucu ggaugccauc     2160

auaguugcau auuccaccuc uucgauuauc aaagcguuca gcgacuuuaa gaaaaaccaa     2220

gagcuuuuga aggcucguuu cuacgccaaa gaacugacgu ccgauaauua uaagcaccag     2280

gugaaguuuu ucgagccguu uaaaggguuc cgggaaaaga ucuuaucaaa aauugacgag     2340

aucuuuguca guacuccccc ucgaaagaga gcaaggggag cccuccauga agagacauuc     2400

uacucugagg auaaaaugau uaagaaguau aacagcaaag aaggucugca gauagcucuu     2460

uccuguggcc gcgugcggaa gaucgggacu aaauacguag agaaugacac caugguuaga     2520

guggauauuu uuaagaaaca gaacaaguuc uaugccaucc caauuuacgu cauggacuuc     2580

gcacugggaa ucuugcccaa uaaaauagug aucacaggca aggauaagaa caauaacccu     2640

aaacaauggc agacuauuga cgaaucauac gaguuuugcu ucagccucua uaagaacgac     2700

cugauccuac ugcagaaaaa gaauaugcag gaaccagagu uugcuuacua uaacucuuuc     2760

gauauugaca ggucccgcau caaaauaaag aagcacgaua auaaauuuga gaaccucacc     2820

aguaaucaaa agcugcuuuu cacaaacaaa gacaagggua aaaaucgaac cgggauucag     2880

aacuugaaga ucuuugaaaa guacaucguc acuccccugg gcgauaaaau uaaggccgac     2940

uuccagccgc gggagaauau cagcuuaaaa acgucuaaga aacacggacu cuga           2994


<210>  2582
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2582
augggcaacu acacccuggg gcucgacauc ggaagcaauu ccauugguug ggcccuugug       60

gaggaugcug ucgaagcacc cagaauaguu gacaugggcg ugaggguauu cccugagggg      120

guggaucggg acacaaaggg acuggaaaaa ucuaagaacg ccacucgccg agaggcgcgu      180

ggcgcuagaa ggacgcacca gcggcgcaga caaaggaaac agcgguuggu ccgcgccuua      240

cgacaggugg gucugcuacc augcgauaag gcagaaguua gaucacucuu ucagaccgac      300

ccguaugaga ucaggaaacg ggggcuggau caucgucuug agcuguacga aauuggacgc      360

gccuucuauc acuugaguca aagaaggggc uuuaagagca aucggaaguc cggcgcucuc      420

aaagaggacg ggacagugaa gaaagaagca ggagagcugc agcgaaagau cgaugaggcc      480

gguugucgca cuauuggcga auacuucgcu gggcuggacc ccgagcagca gagaaucagg      540

ggacgguaua ccuuucgcuc uauguaccaa aaagaauuug aucucuuaug ggccaagcag      600

gucgaguuuu auccuaaggu gcugacagaa gaccuuagau cgaaaguaag ggaugagaua      660

aucuucuuuc agcguccauu gaagucagcg aaagaccuga uuggcgacug cgagcuagaa      720

cccaccgaga agcggugucc ucgagcagau ugguacgcca gaagguuccg ccuccugcag      780

gacaucaaca aucuuauuau ccaaaaccca gaugguacug aacagaaacu gagccccgac      840

cagcgggcug ccuugcucgg ggagcuggga caaagagaaa aguuaacauu ugauaauaua      900

agggcaaaac ugggccuccu ugagacccag aaguucaacg cugaguaccg cauuggccga      960

aaggggaaaa agguugaagc caucaaagga gacguguuug ucuggacgau gcgggguaag     1020

aguguguucg gcccuaaagc gugggaugcc auggcagacg agcguaaggu gucccugaau     1080

gaauuguucg ucgagcuaga agaugacgau cuggcuagaa agcucgagac ugaguauggg     1140

cugaccgaca aacagguuca ggccgcuaug aaggccucuc uuccacaagg auacaugagc     1200

uuuuccagga aagcagugcu gaaguugcuc ccguucaugg aagccggcgc ucugacacau     1260

gaggcgauug accaggcagg uuaucgcaaa gaggaaccca cucagaccga ugccaucgcu     1320

cggguaucau uuccuccaga cuuaagaaac cccaucgugc agaaggcccu guucgagguc     1380

aggaaggugg uuaaugcccu agugcgagaa uacgggaaac cuacacgcau uaagauagag     1440

auggcacggg auguccaagg caguaaaaga cagagggagg aacuccacug gcguaacgaa     1500

gacaaucgca agaaaaacga ggaggugcgg gauagacuga ggaccgacau ccauauugau     1560

gacccaagcc gagaugacgu aaucaaguau aaacuuuggc aggaaucugg aaaaaugugc     1620

cccuacacgg gccggcagau augucaagag gcuuuguuug ggccgaaucc ugaguuccag     1680

guugaacaca uucugcccua ugaucgcucc cucgacgauu cauacaugaa ccugacucuu     1740

ugcgaagugc acgagaacau ccauggaaag agaaaucaga caccauuuga ggccuacggu     1800

agcggcgcag aaagguauga gcagaucaaa caacggauua agguccugcc uuggcccaaa     1860

cgacagaagu ucuugcagca agaaaucaac cucgacaguc acauuucuag agagcugaau     1920

gacacccgcu acauauguag ggaaacugug gcguauuuaa aacagcuggg gguccaugug     1980

cguggaacac ggggcaagau caccgcugag cuaagacacc aguggggucu cgaugggauu     2040

uucuccgagc ugggcguuag gcgcgacgau gaccaccgaa gacaugccgu ggaugcacuu     2100

guagucgccg ugacggacaa cgaacacuug cggaggcugg cuguuagcaa guacgugcuc     2160

gccggaccac gcuucgagac ccccuggucg gcauuucgug augaagcuag aggcaauguc     2220

aaacggauga acgugucuca uaggguaugc cgcaaggugu ccggggcccu gcacgaggag     2280

acaaauuaug gaccuacugg uguucgagac caggcgggcc aagauaucua cgccuauaga     2340

aagaaguuag aagaccugac cacaccaaug gucgagaaaa ucguggaucc ggugguccgg     2400

gacauuguua ggcagcgccu uuugaugaag gggauagauc ccgcugcagc cgacagaaag     2460

aucccuaaag acguguggaa ggaaccacuc uacaugaggc ggacuaaauc agauaagaug     2520

auucagaucc gucgcgugcg aauccaugac gucaaaaaca guauuauagu acugaccgau     2580

gagagcggaa gacccuacag ggcuguggaa ccuggcggua aucaccacgu ugagguguuc     2640

gaguaugucc augggacaaa gaccggcacu cgggaagccg aggugguuac gauguuugaa     2700

gcagugcgca gauccagggc gcgggcccca guaguccgaa gagaccacgg acccggcaca     2760

cgcuucgugu guucucuagc uaucaacgau auggugauga ugccggacaa gagcgggaaa     2820

auggaucugu ucaggauuca gaagauguca gccaguaaac aagucuacuu ucggcaucac     2880

accgcagcuc guaucgacuc cgagagaacu cucauuagga agcaggccca ccuguucaau     2940

ggauauaaag uuagcgugga uccuaccggc gcaaucugcc gcgccaacga cuga           2994


<210>  2583
<211>  3000
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2583
augaagaucc ugggcuucga cauugggaua aacagcaucg gaugggccuu uguggaggau       60

aaucagcucc aagacugcgg ugucagacuu uucaccaaag cugaagaucc caagacaaaa      120

gagucccugg cauugccuag gcggaacgcc cgcucuaauc gacguagauu aggcaggcgg      180

cgcucaagac ugauugcgcu aaagcacauc auuaguaaag ggcucaagcu gaacuaccag      240

gacuauaucg cuaaugaugg agaacuucca aaggccuacg agggcaggcu gauaagcccg      300

uaugaauugc gguacaaagc acucaacgag aagaucgagc ccaaagaccu ggcccgcguu      360

auucugcaua ucgcuaagca ccgagguuau augaauaaaa acgaaaagaa guccucugau      420

aaugagaaag ggaagauucu cucggcauua aaaacuaacg cccugaaacu ugaaaaguac      480

cagucagugg gagaguauuu uuacaaggag uucuuucaga aauacagaga aaauacgaag      540

gacuucauca acauaaggaa uaaggagggc agcuaugaaa acuguguauu ggcuagugau      600

cuggagaaag aacuaaagcu cauucuggag aaacaaaagg aguggggcua cuccuauaau      660

gacaacuuua ucaaagaaau ucuuaaggug gccuucuuuc agcggccucu gaaggauuuc      720

ucuuacuugg ucggggcgug caccuuuuuc gaggacgaaa aacgugcaug uaagaacagc      780

uauuccgccu gggaguuugu ggcucucaca aaaaucauca augaacugaa gucauuagag      840

aaagagagug gagaacuggu uagcucucag auuauaaacg agauccucaa ucauauucuu      900

gauaaggguu ccaucacuua caagaaauuc cgcgaauaua uaaagcugca cgagucaaug      960

aaauucaaga gcuugaaaua cgacaaggac aacguggaaa guaccaagcu aauugaguuu     1020

agaaaacugg ucgaguucaa gaaagcccuc ggcgaacauu cucuguccag ggaggaacuu     1080

gaucagaucg caacauacau cacccugauu aaggacaaug agaaauugaa gaucacucuc     1140

gagaaauaua gccugaacaa ugaacaaauu aagaacuuac uggagauaga uuuuaaugac     1200

cacaucaacc uaucguucaa ggcucucaau cugauucuuc cauugaugaa agaagggaag     1260

cgguacgaug aggccugcaa acugcucaac cugaagacaa aaucuaauaa ccagaaguuu     1320

gacuuccuuc ccgcguuuug ugauuccauc uucgcccagg aacugaccaa uccuaucgug     1380

aaccgagcaa uuucagagua ucgcaaggua uugaacgcuc uccugaaaaa guacggaaaa     1440

augcacaaga uacauaucga guuagccaga gacauuggcc ugaguaaaaa gcuaagggcu     1500

aagaucgaaa aagagcagaa ggaaaauuau gagaacaaua uuugggcccu caacgaaugc     1560

gagaaauuug gucugaaggc aaaugccaaa aacauccuua aguugaagcu guggaaagag     1620

caaaaggagu ucuguauaua cagcgggaaa aagaucucca uugaacaccu ccgggaugaa     1680

aaagcucugg agguugacca uaucuaucca uacucucgca gcuuugauga cucauucuua     1740

aauaaggugc uggucuuuac gaaagaaaac caggagaaac uuaauaagac ucccuucgag     1800

gcguucggag caaacgaaga gagauggagu aagauucagg ccuuggcuca aaaucucccu     1860

uacaagaaaa agaacaaaau ccuggacgaa gccuuuaagg gcaagcagca gcaggauuuc     1920

auauccagga aucuaaacga cacccguuau auuagcacac ugaucgugaa auacacuaag     1980

gaguaucucg auuuucugcc acuugacgag aaagaagaug ugaauuugaa gucuggcgag     2040

aaagggucaa agauucacgu ccaaaccauc aacggaaugc ugacaagugu uuuacggcac     2100

accugggguu ucgcacagaa ggaccgaaac aaucaucucc accaugcccu ggaugcuacg     2160

auaguggccu acagcacuaa cgcaaucauu aaagcguuuu ccgacuucaa gaaagaacag     2220

gagcuucuga aggcuaaauu guaugccaag gaacucacau cugauucgua caagcaccag     2280

gcaaaauuuu ucgagccguu ugagggcuuc agagaacaaa uccugaauca gauuaacaag     2340

cuauuuguaa gcaaaccccc uaggaagcgc gcccggggag cucugcauaa agagaccuuc     2400

uauuccaagg acgaaaugau caagaaauac aauucacagg agggagugga aauagcccuc     2460

aacugcggca agauuagaaa aaucgguacu aaguacgucg agaaugauac aauggugagg     2520

aucgacauuu uuaaaaagca gaacaaauuc uaugcaaucc caauuuacac cauggauuuc     2580

gcucuggggg uucuucccaa uaagauagug aucauuggca aggacaaaaa gggaaacccu     2640

aaacaauggc aggagaucga cgaaaguuau gaguuuuguu ucucuuugca caaggaugac     2700

cuggucuuaa uucagaaaaa ggauaugcag gaaccagagu uugccuacua uaauggcuuc     2760

gacaucagca acuccucaau augcguggaa aagcacgaua auaaauuuga gaaccucacg     2820

gacaaucaac gccugcuuuu caccaacgcg gaggggaacg uaaaggccaa aaagaucggg     2880

auucaggguc ugcgaaucuu ugaaaaauac auuaucacac cccuaggcga gaagauaaag     2940

gcugauuuca aaccgcggga agacauugca cucaagacua gcaaaaagca ugggcuguga     3000


<210>  2584
<211>  3003
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2584
augaagaucc ugggcuucga cauugggaua aacagcaucg gaugggccuu uguggagaau       60

gaugaacuca aagacugcgg ugucagaauu uucaccaagg cugagaaccc caaaaauaag      120

gaaucccuug cacugccuag gcggaacgcc cgaucuucac gccguagauu gaaaaggcgg      180

aaggcgcgcu uaaucgcuau uaagagaauc cuggccaaag agcuaaagcu caauuuuaaa      240

gauuacguug cagccgacgg cgaacugcca aaggcuuaug aggggagucu ugcaagcgug      300

uacgagcuga gguauaaagc cuugacacag aagcucgaaa cuaaggaucu ggcucgggua      360

auacugcaca ucgccaaaca ucgcggauac augaacaaga augagaaaaa guccaacgac      420

gcgaaaaagg gcaaaauucu cucugcauua aagaauaacg cccugaaguu ggaaaauuau      480

caaucggugg gugaguacuu cuauaaagag uuuuuccaga aguacaaaga aaacacgaag      540

aauuuuauca aaauucgaaa caccaaggau aauuacaaca acugugucuu gucaagcgac      600

cuggagaagg aacuaaaauu cauccucgag aagcagaaag aauuugggua uaaguacagu      660

gagggauuca uaaaugagau ucugaaagug gccuuuuucc agagaccgcu uaaggauuuu      720

ucccaccugg uuggcgcuug cacauucuuc gaagaggaaa acagggcaug uaaaaauucu      780

uauagcgcuu gggaguuugu ggccuugacu aaaaucauua acgaaaucaa gucccucgag      840

aaaaucucag gcgagauugu caguacccaa acaauaaaug aagugcugaa cuuaauccug      900

gacaagggga gcauuaccua caaaaaguuc cggucuugca ucaaucucca ugaguccaua      960

ucauuuaaga gccuuaaaua ugauaaggaa aacgcggaga augccaaacu gauugacuuc     1020

cguaaguugg uagaauuuaa aaaggcacua ggaguucaca gucugucucg ccaggagcuc     1080

gaucagaucu ccacucaugu gacacugauc aaagacaacg ucaaacuuaa gaccgugcug     1140

gagaaguaca auuugagcaa cgaacagauu aauaaccucc uggagaucga guucaaugac     1200

uauauuaacu uaucguuuaa ggcucugggu augauacuac cccucaugag agaaggcaaa     1260

agguacgaug aggccuguga aaucacgaac cugaagccua aaacugugga cgagaagaag     1320

gauuuccuuc cagcuuuuug cgacucuauu uucgcccacg aauuguccaa ucccgucguu     1380

aaccgggcaa ucucagagua ccgaaaagug cugaaugccc uccugaagaa auaugauaag     1440

guacacaaaa uccaucuuga acuggcucgc gacguggggu ugaguaagaa ggcgagagag     1500

aaaauugaga aggaacaaaa agagaaccag gcagucaaug ccugggcucu caaggaaugu     1560

gagaacauag gacugaaagc cagcgcaaag aauaucuuaa agcugaaacu auggaaggaa     1620

cagaaagaga uuugcaucua cuccggcaac aagauuucua ucgagcaccu caaagaugaa     1680

aaggcccugg agguggacca uauauauccu uacagcaggu cauuugauga caguuucaug     1740

aauaaaguuc uuguguuuac caaggaaaac caggagaagu ugaauaaaac accauucgag     1800

gcuuucggua agaacaucga aaaauggucc aagauucaaa cucuggccca gaaucucccc     1860

uauaaaaaga agaacaaaau ccuggaugag aauuuuaagg acaaacagca agaagacuuc     1920

auuagccgga acuuaaacga uacccgcuac aucgcaacac ugauaaccaa guauacgaaa     1980

gaguaccuua auuuucuucc ucucaacgaa aaugagaaca cuaaucugaa gucuggggag     2040

aagggaucaa aaauucacgu ccagacagug aacggcaugc uaaccagugu acugagacac     2100

acuuggggcu ucgacaagaa agauaggaau aaccaucucc accaugcgcu ggacgcuauc     2160

auuguugccu acagcacaaa uuccaucaua aaggcauuuu cugauuucaa aaagaaccag     2220

gaacuuuuga aggcccguuu uuaugcuaaa gagcugaccu cggacaauua caagcaccag     2280

gugaaauucu uugaaccauu caagggguuu cgggagaaaa ucuuaagcaa gauugaugaa     2340

aucuucgucu ccaagccgcc ccgaaaaaga gccaggggag cacuccauaa ggagacguuu     2400

uauucagagg acgaaaugau uaaaaaguac aacaguaaag agggucugca aaucgcucuu     2460

ucuuguggcc gcgugcggaa gauagggacc aaauaugucg aaaaugauac aauggugaga     2520

guugacauuu ucaagaagca gaacaaauuc uacgccaucc cuaucuaugc gauggauuuu     2580

gcccugggaa uuuugccaaa uaagaucgug guagucggca aagacaagaa caacaauccc     2640

aaacaguggc agacuauuga ugagagcuac gaguucugcu uuucccucua caagaacgac     2700

cugauaaugc uacaaaagaa agacaugcag gaagcugagu ucgcauauua caaugguuuu     2760

gauaucucaa ccagcucuau uuguguggaa aagcacgacu ccaaauucga gaaccugaca     2820

aguaaucaga agcuccuguu uagcaacgcc aaagaagggu cuguuaaggu ggagaaucuu     2880

ggcauccaga acuugaagau uuucgaaaaa uauaucauaa cuccucuggg agauaagauc     2940

aaagcugacu uucaaccaag ggagaauauu uccuuaaaga ccucaaagaa gaacggccuc     3000

uga                                                                   3003


<210>  2585
<211>  3003
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2585
auggccagag cucagaggcg gcugaucagc cgccgagcaa accguaugaa uaagcucaga       60

aggcuucuga aacaagagaa cguguugcag gccaaugacu acgaugaaaa gggcuuaauu      120

auagggcugc ccaaccaggc gugggagcua cgggcucagg gacucgaccg caaacuggaa      180

ccuaaggagu gggccgcagu ccuuaucaau cugguuaaac acagagguua uuugucccaa      240

aggaagaacg aaucucagac cgccgauaag gagcucggcg cucugcuguc agggaugaau      300

cauaaccacc agcucuuaca gagucaaagc aaucaguacc ggacaccagc agagcuggcc      360

auuaaacagu ucgcuacuca ggacggacau auccgcaacc aacgaggcgc cuauacgcac      420

accuuuaaua gacuugauuu gcagaacgaa cugaaucagc uauucgacgc gcaaacaagg      480

cucgguaacc cgcauguguc cgggucucug aagacugcaa uuaaugaucu ucugaugaac      540

cagaaagccg cuuugucggg agacgccauc cucgagaugc ugggccggug caccuuugaa      600

cccucagagu acaaggcagc uaaaaacaca uauagcgccg agcguuucgu augguuaggc      660

aagcugacca aucuccgcau acaggauaac gggcuugaaa gugcgcuguc cagagagcag      720

agggaauugc uacugaauga gccuuacaac aagacuaaac ucacauauca acaggugcgg      780

aagcugcuuc gacugccaga aaccgccuuu uucaaaggau ugcgcuacgg uaaggacaau      840

gcagagucug cuacguuuau ggagaugaaa agcuaccacg ccaucagaaa gucccucgaa      900

aacaaaucac ugaagaguga gugggaaaag uuaaaaacuc agcccaagcu gcuagaucuc      960

auuggcaccg cuuucagcau cuauaaaaca gacgaugaga uugccgggug ucugaagaau     1020

cagcuuccug aaaacgucau cauagcauug caagagcagc ugaauuuuuc uaacuucaug     1080

gagcucuccc ugaaagcccu ugaaaagcug uugccacuca uggagcaggg acugagguac     1140

gacgaggcuu gcacucagau uuauggcaau cggcacgaaa agaucgcgga caaaaccgaa     1200

aaguuuuuac ccaacauucc ugcagauaaa cugcgcaauc cagugguucu aagaacacuc     1260

acccaagcca ggaaggugau caacgcuauc auucgucggu acgguucacc ggcccgaguc     1320

cauauagaga cggggagaga ggugggaaag agcuucagug acagggauaa gcugaagaaa     1380

cagcaggaag agaaucagga acaacgccgg agagcaaucg acgaguggaa gcaguuuuuc     1440

cccaacgccg cucacgagcc uaaagccgua gauauucuua aguugaggcu guaucagcuc     1500

caacagggca aaugucugua cucuggcuua ccaaucgacg uucgccgacu gcccgaaaag     1560

ggguaugugg agauagauca ugcacuuccu uucucccgga cuugggacga uagcuuuaac     1620

aauaagauuu ugguccucgc gucggaaaac cagaauaaag gaaaccagac accauacgag     1680

uaccugcgug gugcugacaa uucugaaucc uggagacuau ucguggagaa cgugcaaaau     1740

ugccaguuuu cacccaacaa gaaacagaag aucauggcca aacagcugga ugagaagggc     1800

uuccucgaaa ggaaucugaa cgacacccgc uaugucaguc gguuucuuug ucaauucauc     1860

agcgagcacu ugcaccugac ugggaaagga aagaagagag uuuuugcauc caauggccag     1920

auuacagccu uacucagggg ucgauggggg cugucuaaaa accgcgaaga gaaugaccgg     1980

caucacgcug uggaugccgu aguggucgca ugcagcaccg uggcuaugca gcagaagauc     2040

acgcaauucg uuagauacca gaaaggcaac guguuuaccg gagaaaggau ugacaaggag     2100

acaggcgagg ucauaucaau ccauuucccg acuccuuggg aacacuuucg ugaggaagug     2160

augauucgcg uauucgauga caauccccuu cggacccugc cagagaaauu gccuaacaga     2220

cccgaagccc uccaugagua uguuaugcca cuguuuguga guaggaugcc cacacgaaag     2280

augacugggc agggacacaa ggagaccauc aaauccgcga agcggcuaga ugagaaaauc     2340

agcguccugc gcgugccucu cacacaacug aaacuuaacg acuuggaaaa uauggucaac     2400

agagaaaggg agccagcccu guacgaggcu uuaaaagcac ggcucgaaca guucaagaau     2460

gauccggcca aggcuuucgc cgagcccuuu uucaaacagg guggccagca agugaaggca     2520

guucgagugg aaauuguaca gaaaucuggg augcugcuua gaaagauaaa cggagucgcg     2580

gacaauggcu caauggugcg cguugaugug uuugagaaaa gugguaaguu cuaucugguc     2640

ccuaucuacg ccuggcaagu ggcugaaggg auucuaccaa acuaugccgu aguggcacac     2700

aaggacgaga gcgauuggau ccucauggac gaguccuacc auuuuaaauu cgcucuguac     2760

aagaaugauu ugcuggaagu ucagaccaaa aagggcggaa uuaugggcua uuuugccucu     2820

uuagacaggg caacuggggc caucacgaua cgugagcacg accgggauaa aaagaaaugu     2880

aaggacggag aacauagauc gaucgguguc aagauggcua gcuccuucaa aaagcuccag     2940

guggaugagc ugggcaaaaa cauucacgaa acaaggucac gcaagcgacc caauaucggg     3000

uga                                                                   3003


<210>  2586
<211>  3012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2586
augagaaucc ugggcuucga cauugggaua aacagcaucg gaugggccuu uguggagaau       60

gaugaacuca aggacugcgg ugucaggauu uucaccaaag cugagaaccc caagaauaaa      120

gaaucccuug cacugccucg gcgcaacgcc cgaucuucac guagaagguu gaagcggcgc      180

aaagcgagau uaaucgcuau uaagaggauc cuggccaagg agcuaaaacu caauuacaag      240

gauuauguug cagccgacgg cgaacugcca aaggcuuacg aggggagucu ugcaagcgug      300

uaugagcugc gguacaaagc cuugacacag aaccucgaaa cuaaagaucu ggcucgcgua      360

auacugcaca ucgccaagca ucgaggauau augaauaaga acgagaaaaa guccaaugac      420

gcgaaaaagg gcaaaauucu cucugcauua aagaacaaug cccugaaacu ugaaaacuac      480

caaucggugg gugaguauuu uuacaaggag uucuuucaga aguacaaaaa gaauacgaaa      540

aacuucauca agauuagaaa uaccaaagau aacuauaaca auugugucuu gucaagcgac      600

cuggaaaagg agcuaaagcu cauccuggaa aaacagaagg aguuugggua caacuauagu      660

gaagauuuca uaaaugagau ucuuaaagug gccuuuuucc agaggccgcu gaaggacuuu      720

ucccacuugg uuggagcuug cacauucuuu gaggaagaga aacgggcaug uaagaacucu      780

uacagcgcuu gggaguucgu ggcccucacu aagaucauua augaaaucaa aucccuggaa      840

aagaucucag gcgagauugu ccccacccaa acaauaaacg agguguuaaa ucugauccuc      900

gauaaaggca guauuaccua uaagaaauuc cguagcugca ucaaccuuca ugaaucuaua      960

uccuuuaagu cacugaagua cgacaaagag aaugcggaaa acgccaaguu gauugauuuc     1020

cgcaaacugg uagaguuuaa gaaagcacua gggguucaca gccucaguag acaggaacug     1080

gaccagaucu cuacucauau cacacuuauu aaggacaaug ugaaacugaa gaccgucuug     1140

gagaaguaua accucuccaa ugagcagauc aacaaucugu uagaaauuga guucaacgau     1200

uacauaaacc ugagcuuuaa agcucuagga augauccucc cucugaugag ggaagguaag     1260

cgguacgacg aggccuguga gauugcuaau cuuaaaccaa agacggugga ugagaaaaag     1320

gacuucuugc ccgccuuuug cgauucgauc uucgcacacg aacugucuaa cccugugguc     1380

aaucgagcca ucuccgaaua ucgcaagguu cucaacgcuc ugcuuaaaaa guacggcaaa     1440

gugcacaaga uucaucugga guuggcgaga gacguagggc ucaguaaaaa ggcaagggaa     1500

aagauagaga aagagcaaaa ggaaaaucag gccgugaacg cuugggcccu gaaagagugu     1560

gaaaauaucg gauuaaaggc aucagccaaa aacauucuga agcuaaagcu cuggaaagag     1620

cagaaggaaa ucugcauuua uagcggcaau aaaaucucca uagagcaccu gaaggaugag     1680

aaagcucuug aagucgacca uaucuaccca uauucucgga gcuuugauga cucauucauu     1740

aacaaggugu ugguuuuuac uaaagagaau caggaaaagc ugaacaagac ccccuucgag     1800

gccuuuggua aaaauaucga aaaguggagu aaaauucaaa cacucgcaca gaaccugccu     1860

uacaagaaaa agaauaagau cuuagaugag aacuucaaag acaagcagca agaggacuuc     1920

auaucccgca accugaauga uacuagauau auugcgaccc uuaucgcuaa auacacaaag     1980

gaauacuuga acuuucuccu gcuaagcgag aaugaaaacg ccaaucugaa aucuggggag     2040

aagggaucaa agauucacgu gcagaccauc aguggcaugc ucacgagcgu ccugaggcac     2100

acuuggggcu ucgacaaaaa ggaucguaac aaucaucuuc accaugcauu ggacgccaua     2160

aucguggcuu auuccacaaa cucuauuauc aaagccuuuu cggauuuccg gaagaaucag     2220

gagcuguuaa aagcacgauu uuacgcuaag gaacucacca gcgacaacua uaagcaccag     2280

guaaaauucu uugagccauu caaguccuuu agagagaaaa uucugucaaa gaucgaugaa     2340

auauucguua guaaaccgcc caggaagcgc gcccggagag cgcuucauaa ggacacuuuu     2400

cacucugaaa auaaaauuau cgauaagugu agcuacaacu ccaaagaggg gcugcaaauc     2460

gccuugucau gcggaagggu gcgcaagauu gguacaaaau augucgagaa ugacaccauc     2520

gugcgagucg auauuuucaa gaaacagaac aaguuuuacg cuauaccuau cuaugcaaug     2580

gacuucgccc ucggcauucu gccaaauaag aucgugauua cggggaaaga uaagaacaac     2640

aaucccaaac aguggcagac caucgacgaa agcuacgagu ucuguuuuuc ucuauacaag     2700

aacgaccuga uacuccugca gaaaaagaau augcaagaac cugaguucgc uuauuacaac     2760

gauuuuucca ucaguacaag cucuauuugc guugaaaagc acgacaauaa auucgagaac     2820

cuuacuucca aucagaaguu gcuguuuuca aacgccaaag agggaagugu gaagguagag     2880

agcuuaggca uccagaaucu caaagucuuc gaaaaguaua uuaucacccc acugggugau     2940

aagauaaaag cagacuuuca accccgggaa aacauuucgc uuaagacauc caaaaaguac     3000

gggcugcguu ga                                                         3012


<210>  2587
<211>  3012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2587
augaucgacg agagccugac cuucggcauu gaucucggga uaggauccug cgguugggcc       60

gugcuuagaa ggcccucugc uuuuggccgg aaggggguca ucgaaggaau gggcucaugg      120

uguuucgacg uuccugagac aaguaaagaa cgcacuccaa cgaaccagau ucgacguagc      180

aauagacugu ugaggcgggu gauccgcaga aggcggaacc gcauggcagc cauucgaaga      240

uuacugcacg cggcuggucu acucccgucc accgauucug acgcccugaa gaggcccggg      300

caugauccuu gggagcuucg ggcacgugga cuggacaaac cauugaagcc cguagaauuu      360

gccguggucc ucggccacau cgcuaaacgc agaggcuuca agucggcagc caagaggaaa      420

gcuacaaaua uaucaagcga ugacaagaaa augcugacug cccuggaggc gacccgggag      480

cgacucgggc gcuacagaac agugggagaa auguuugcaa gggauccuga cuucgccagu      540

cggcgcagaa acagggaggg uaaguaugau cguaccacug cucgggacga uuuagaacau      600

gagguucacg cccuguuugc agcucaacga agacuuggcc agggguucgc cuccccagag      660

uuggaagagg cguuuacagc cucugcauuc caucagaggc ccaugcagga cagcgaacgc      720

cuggugggau uuugcccuuu cgagcggacc gaaaaacggg cugccaagcu aacgccaucc      780

uuugagaggu uccgccuccu ggcucgacuu cugaauuugc ggaucacuac cccggacggc      840

gagcgucccc ucacagucga ugaaauugcc cuggugacua gagacuuagg uaagaccgca      900

aaacugucaa ucaagagggu acgcacacuc auugggcuug aggauaacca acgguucacc      960

acgaucagac cugaagacga ggauagggac auaguugccc gaacuggagg cgcuaugaca     1020

ggcaccgcga cucugcgcaa agcauugggg gaagcccuau ggacagauau gcaggagcgg     1080

ccagagcagc uggacgcuau ugugcagguc cucagcuuuu ucgaagccaa ugagaccauc     1140

acggaaaagc ugagagagau uggacuuacc cuggcagugu uggaugugcu ccugacagcc     1200

uuagacgcug gugucuuugc caaauucaag ggcgcagcgc acaucagcac uaaagcugcc     1260

aggaaccugc uaccccaccu cgaacaaggg cgucgcuacg augaggcaug uaccauggcc     1320

ggauaugacc augcugccuc ucggcugucc caccauggcc agaucguugc aaagacacag     1380

uuuaaugcuc uugugacuga gauuggugaa ucaauagcca acccuaucgc gagaaaggcc     1440

uugauugagg ggcugaaaca gaucugggcu augaggaauc acuggggccu cccaggaagc     1500

auacacguag aacuggcacg agacgugggc aacaguauug agaagcggcg cgagaucgaa     1560

aaacauaucg agaagaauac cgcccuuaga gcuagggaac ggcgagaggu ccacgaucug     1620

uuggaccucg aagaugugaa cggggacaca cuguuaagau accgccugug gaaagagcaa     1680

ggagguaagu gccuauauac cggcaaggcc auucauauca ggcagauugc agcgacugau     1740

aauucuguuc agguggacca cauacucccc uggucccguu ucggggauga cagcuuuaac     1800

aacaaaacgc ugugucuugc cucggcuaau caacagaaga aacggucuac accguacgag     1860

ugguuguccg gacagaccgg cgaugccugg aacgcauucg uccagagaau cgaaacuaau     1920

aaggagcuga gggguuuuaa aaagcgcaac uaucuccuga agaaugcuaa agaagccgag     1980

gaaaaguucc gaucaagaaa cuuaaaugac acccgguacg cagccaggcu guuugcugag     2040

gcggugaaac uuuuguacgc cuucggggag cgccaagaaa agggcggaaa ccguagagua     2100

uuuacacggc cuggcgcacu caccgcugcc cugaggcagg ccuggggagu ugagagucug     2160

aaaaagcagg augggaagcg cauuaaugac gaucgacauc acgcucuaga cgcacucacu     2220

guggccgcgg ucgacgaagc ugagauccag agacugacaa aaaguuucca cgaaugggag     2280

caacaggguc uuggccggcc cuugaggcgc guggagccac cuugggaauc cuucagagcc     2340

gaugucgagg caacguaucc cgaaguguuu guugccaggc cagagcggcg ucgcgcucga     2400

ggggagggac augcagccac caucagacag gugaaggaaa gggagugcac ucccauugua     2460

uucgaacgga aagcggucuc uagccugaag gaggcugacu uagaacgcau aaaagauggc     2520

gagagaaacg aggccaucgu ggaagcaauu aggucaugga ucgccacagg ucggccugcu     2580

gacgccccac cgcgaagucc cagaggggau auuaucacca agauacgccu cgcaacaacu     2640

aucaaagcug ccguuccugu gaggggcgga accgcgggca gaggggagau gguccgugcc     2700

gacguguuuu ccaagccaaa uagaagggga aaggaugaau gguaccuggu acccguguau     2760

ccucaccaga uuaugaaccg caaagcaugg ccaaagcccc cgaugcggag caucguugcu     2820

aauaaagacg aggaugaaug gacggagguc ggcccugagc aucaauuccg auuuucucuu     2880

uacccaagau caaacauuga aaucauaagg cccaguggug aggugauuga aggguauuuc     2940

gugggacugc accgcaauac cggcgccuug aucccuacac ccgucggucc agacagcuac     3000

guuauugcuu ga                                                         3012


<210>  2588
<211>  3024
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2588
augaaggugc ugggcuucga caucgggauu aacagcauag gaugggccuu ugucgagaau       60

aacgaacuca aagauugcgg uguuagacuu uucaccaagg cugagaaucc caaaacaaag      120

gaaucccugg cauugccuag gcggaacgcc cgaucuucac gagugcguuu aagaaggcgg      180

cgcaguagac ugcuaagccu caaacacauc auuuccaagg aguuuggccu gcauuacgaa      240

gacuauaucg cgcuugaugg gaaucugcca aaggcuuaca cuggaaaaau ugagucuauc      300

uauaaguuga gguacgaggc ccucucgcag gcaauaucaa aagaagaccu ggcccgggua      360

auccugcaca uugcuaagca ucgcggcuau augaacaaaa aucaaaagac gagccaggau      420

agugagaagg gugcaauccu cuccgccauu aaagaaaacu cuaauaagau caaacacuac      480

cagaccgcug gggaguauuu cuacaaggag uuauuugaaa accugcgaga cggaacaaaa      540

gaguucaaga auauaagaaa caaagccaug aagaaugaaa agacuggcaa cauggagagc      600

accuacuaua auacaauucu ugcguccgau uuggaaaaag agcugaagcu aauccucgag      660

aaacagcaag cauaccauaa cuauucagac accuuuauua aggaagugcu ggagaucgcc      720

uucuaccaga ggccgcuuaa agauuuuagu caccuggucg gcagcuguac uuucuaugaa      780

aacgagaagc gggcuugcaa aaauucuuac uccgccuggg aauuuaucgc auuggggaaa      840

auuauaaaca cacucgaguc aaucaagaaa gacaccggag agauuuucag caaugaaauc      900

auaacgcaga uucugaagga gguguuagau aaagguaguc ugucuuauac uaagcuccgu      960

aagaugauca accuugacga aaaaguuaag uuuaaaggcc ugaaguacga uaaagagggg     1020

guggaaaaga ccaauuugau cgaguucaag aaacuaaagg aguucaaaaa ggcucuggga     1080

gaacacaacc ucgagcgcaa aacacuggac gaaauugcca cucauaucac ccuuacaaag     1140

gacgagaauc agcugaaaaa gcaauuggag caguacgcgc ucucccagaa ccagauugau     1200

gcccuggcag aauuagacuu uaaugaucac auaaaccuga gcuucaaggc ucuauaugag     1260

auccuccccc ugauggaaaa aggcaagaga uacgacgagg ccuguaauga acuugguuug     1320

aaagcuaaga ccucgaacca aaaaucugag uuucugccug ccuucugcga uuccauuuuu     1380

gcacaggagc ucacgaaucc aaucgucaac agggccaucu cagaauaucg gaaggugcug     1440

aaucgacuuc ugaagaaaua cgggaagaug cauaaaauuc acauagaguu ggcucgcgac     1500

gcgggacuca guaaggaagc aagaaacaaa aucgagaagg aacagaagga gaacaaagau     1560

auuaaugaca aggccgagaa aacuuguaag gaguucggcc ugaaacccaa cgcuaagaau     1620

aucuuaaaga uuaaacugug gagggaacaa ggcgaaaucu gcgccuauag cgggaagaaa     1680

auaacaaucg augaccuaaa ggaggauaaa gcacucgaag uagaccacau uuacccuuau     1740

ucccggucuu acgacgauag cuacaacaau aagguucugg uguuuaucaa agagaaccag     1800

cuuaaguuga aucagacccc auaugaggcc uucggaucaa accaggaaaa guggaguaaa     1860

auucaagucc uggcucagaa gcuccccuac aaaaagaaaa aucgcauccu ggacgagaac     1920

uuuaagaaua aggaucagau agaguucauu uccagaaacu uaaaugacac uagguaugcc     1980

acaagccuga ucgcaaaaua caccaaggaa uaucuugagu uuuugccucu cucugaugac     2040

gaagauauuu cacugaaaag uggugagaag ggcagcaaaa cgcaugugca gaccaugaac     2100

gggaugcuaa cauccgugcu gcgucacgcg uggggauucu cucaaaagga ccggaauaac     2160

caucuccacc augcucugga ugccaucaua aucgcauacu cgacuaacag cauuaucaag     2220

gccuuuuccg acuucaaaaa gaaucaggaa auucuuaaag cuaaguugua ugccaaaaag     2280

cugaccucag aggaauacaa gaaccagagu aaauucacag gcuuugagaa guaccgaucu     2340

aaaaucuuag agaagcucga uagcauauuc gucuccaaac caccgaauaa gaacacuaag     2400

ggugcacugc acgaacagac cuuuuauuca uaccaagaca uucuugagga auauaaaaca     2460

caggaggggc ugaagagagc uuuggaaugu ggcaaaguua ggaagaucgg aaccaaauac     2520

gugguaaaug aucgcauggu gcggcucgac aucuucaaga aagacaacaa guuuuauggc     2580

auucccaucu acacuaugga uuucgcccug gggguccuac cuaauaaggu gguuacgauu     2640

ggaacagaca aagauaagau aaagaaggag uggaaaacca ucgacgagac uuaugaauuu     2700

ugcuucagcc uguacaaggg ugaucucgug cugauucaga agaaagagau gcaggaaucu     2760

gaguuugcgu acuauaacgg cuucgacauc uccagugcca gcauuucucu ugaaaagcac     2820

gauaauaaau uugagaacuu gaccgagaau caaaagcugu uauucccaaa agcuaaggaa     2880

ggggacgucg aggugagagg aaucggcaua cagggucuca agguauuuaa aaaguacaug     2940

guuacacccc ugggggaaau ccaggagacc aggacugagg cacgccaaaa cauucagccu     3000

cgcaaaaagc auggccuucg guga                                            3024


<210>  2589
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2589
augagcgacu ccucuuacgu gcugggccuc gaucuuggga ccaacucaau cggaugggcc       60

ugcgucagac ugaauaaggg uaaacccgac ggcuuguuag cucagggggu uaggauuuuc      120

gaggcaggac uggaaauaga ugccuauggc cgggguacac cucuaaacac ugagcgccga      180

aaugcgcguc aaagaaggcg gcuccacgac cgcagaaaga ggcggauccu gaaagugcgc      240

cgaauccuuc aggaagcugg gaugcugcca gccaacaagc cggaucauaa agacgcagag      300

uggaagaugu ugcucgccag aaaucccuac aggcugcggg cuaaggcacu ggaugaacag      360

auugaccuca aagagauugg auuauguauc uaucaguuug cccaccgucg cggcuuccug      420

aguaacagca agauacuuaa aaaggaugac gauagaggca aauugaagga cgcuacgaag      480

gccuuugcgg aggauaugga acaaaaaggg gcaaggaccu ggggagagua ccugucccau      540

cuagacccuc acgaacagcg gauccgaaca aauuauacuc uccgcgagug guacaccucu      600

gaguucgacc ugauuuggga aaagcagcag ucguuuuauc cacauguacu uacagaugac      660

cuguacaaaa gagugaagga ggccuuguuc uaccaaaggc cccucaaauc acaggcucac      720

cuguuagguc agugcgaacu ggagccuggc cggaagcgcg ccccauuugc acuccuuaug      780

gcucaggagu ucagacuguu gcaaagccua aaccaccuga gggucgugag uuccaccggg      840

gaugaacguc uccugacuga cgaggaacgg aaacaggccc uugcgcuguu gcgaucuaga      900

ggaaaucaga aguuuacaac ccucaggaag guucugaacu uacgcggcac gaaauucaau      960

cuggagcggg guggggaaaa gacuauccaa ggaaacaaua ccgccgcagc ucuaggcaac     1020

cucuuuggcg aggccuggga ucauuucagc uccgaacaga aaaaucagau uguggaggac     1080

agacugacaa ucggggauac ugacgcucuu auaaggcgca ugaagaaaac cuggggauug     1140

gauucaguca cagccgagau guuuagugaa accgagcugg aagacgguua uuguggcuuc     1200

agccgaaagg cacucgagaa gcugcuuccc cuguuggaac gggggcacgc ccucauggag     1260

gcugugcagg cggcauaccc ugauuaucaa aagaaccugc cacguacgga cacuuuaccg     1320

ccccuggaga accuaagaaa uccuauugua cagaggacac ucucugaacu gcgccggguu     1380

gugaacgcca ucauuaagga guacggaaaa uccgacaaga ucagaaucga acuugcuagg     1440

gauaugaaac agaccgccaa gaaucgagag aagucauggg accgcaugcg ggucagagag     1500

aggcagcgua aagaagcagc caagaaaauu uuggaggaaa cuggcaaccc agcuccccgc     1560

cggcaugaga uagaaaaggu gcugcucgcc gaggagugca auugggaaug uccuuauaca     1620

gguaaaagaa ucugcaugaa cacccuguua ggggaugcac cacaauucga cauugagcac     1680

aucauacccu uuagcaggag uuucgauaau ucuuuuucca acaagacgcu gugugacgug     1740

aauuacaacc gaaaugucaa gggcaacaaa accccguaug aaagcgcguc ggagacagaa     1800

cacgaggaga ugguuugccg ggugaagcgc uucacuggag auuacaaaga aauuaaacuu     1860

gacagauuua auaccaggga gacagauucu cgggcugacu ucaucgaaga guuuuccacu     1920

ucacaguuga acgauacccg auacgccagu agacucgcag gcgaauaucu ggggaaacua     1980

uacggaagcg aguggcgcaa gcauauccag auugccaaag guggcacaac caaguaucug     2040

agggacgcuu gggaucucaa ucguauccug uccgacgggg gaacuaaguc ucgggacgau     2100

cacagacauc acgccguaga cgcagugguc guggcucuua cgacaccuca gaccguuaaa     2160

gccuugagcg augcgaacca aagggccccc gagcaucgcc gauacgcuuu cucagacaug     2220

ccacugccuu gggaaacuuu uagacaggau guggagcagg caauuaacag uauaaccguc     2280

ucccacaagg ugugucggaa agccaauggc caguuacaca aggaaacauu cuauggucuc     2340

aucaaagaca ggaagaccgg ggauacucgc acaguacugc guaagccccu ucaugagcug     2400

acggacaaag agauuaagag agcugaaauc guugauagcu cuauccggaa auugauugag     2460

caaaagcuca aagaccugga ucagccaccc aagaccgccu uuaaggacag gaacaaucac     2520

ccuuucauaa ucgauaaaaa cggcaauaag cgcuuuauuc auaaagugcg agucuaccag     2580

gacgcaaagc cagugaaaau cgcggaaaac agacacguca agcuaggagg caaucaccau     2640

cuggagauuu ucgaagugau cgacaagaaa gggaacccga cuuggaagug cgagguuaua     2700

ucacucuucg auacacggca aagggugaaa gagaagcgcc ccguaaucgu cagacaggac     2760

cagaauggaa acccucugaa auuuagucuu agcauuggug aagccgugca guuggauugg     2820

aauggcggga gggagaccgc uguugugcaa aagaugucca aaacaccaaa cacugucgaa     2880

uauguguucc ggaagcacaa ugacgcccgu aaggcagcug auauccccua caaagagacc     2940

auuacgguaa agucugacuc ggcccuguuu gcaagcaacu uacagaaaau cgugauaacc     3000

ccuacaggac ucauucgccg agccaaugau uga                                  3033


<210>  2590
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2590
augaagaaca gaaaugugau cggcauugac gucgggacca acagcauagg augggcccug       60

guugaggaaa augagaacgg ucucccccag ucccuuaaag cugcaggcgu gaggaucuuc      120

caagaagccg uagaggcgaa gacacggacu ccuaaaaauc acaagcgccg acaggcucgu      180

cuggccagaa ggguguuggc acggcgcgcc agaaggaaac agcgguuaca gaaccugcua      240

cucaagcaug ggcugcuucc aggaggcguc acggauccgg ugcaacgcga agcugcauuu      300

accacacugg gugaccccua ccaguugcga gccaaggggc ucaaucugcc ucugggacuc      360

cacgaguucg gcagaguuuu auuucagcug ggcgcuaggc ggggguuccu uucuaaccgu      420

aaaacucgcu auggacauuu gaauaagcug gaugagcuag ccgaccucau ugaagcggag      480

gaugcagccc caaaaaccgg ugacgcugaa gagggcauca uuagaaagga gaucggggaa      540

cugaggcagg ccaugucagc agcuggaagu cggacacuug gcgaguaccu ggccgguuug      600

gcgaaagagg aacgaaagcg cugcagacac accgaaaggg ccaugcucga ggaggaauuu      660

gagcggcugu ggauggugca agcacgcuuc caucccgaau uaagcgauga gcugaagguc      720

caggugcaca cuguaauauu uuuccagaga ccucucaaau ccaacagggc uucucuuggg      780

aaguguucgc uggaaccauc acgucggaaa gccgcucgag ccuggcccga ggcacagaga      840

uuuagguugc uacaagaccu gaauaaccuc gagauccugg auccucgcac aggagaacuu      900

cggagacuga gggacaccga gcgcgccauu uugcucaaug cucuggaaca gcagaagagu      960

augacgugga acgcgguucg gaaaaaguua ggccugcacc aaggcgagaa auucaaucua     1020

gaaacugggg caaaggccga gaccggacuc aagggucugc agacacuugc uaguuugcau     1080

gccauccugc agggcugguu ugaugagcuc ggggacaaac gggaucugcu uauugaagac     1140

cugaucacua uagaggacuc cgcaggauug uaucguagac ucagggaaca cuggggcuuc     1200

gaucgcgaga ccgccuuucg gcuggcuaca uuagagcugg aaccagguua cgccucucua     1260

agccucaagg caauuagaaa acugcuuccg cauauggggg cgggccacag guauuccgag     1320

gcucgacagc aagugggaua cggcuaugaa accgggcagc gcgacuugca ggagcggcug     1380

ggagaacccc cugauccaag aaaccccguc guggacaagg cccuccacga gcugaggcgu     1440

gugaucaacg cauuaauuca ucgcuucggu cggccugaug ucguuagagu ggagcuggcc     1500

agggaccuua aauuggcuaa gcgagauaag gaacggcucg agaaacagaa ucgcgaaaac     1560

caaagacuga auaggaaggc cgaggcagcu cuagccgaac ugggccucgg gcagccauca     1620

cgggacgauc ugaucaaaua ccgacuuugg gaggagagua agggauugug ccccuacacg     1680

ggcagagaaa ucggucugag cacuuuauuc ucuccggagg uagacgugga acacauuaua     1740

ccuuauuccc gcugucucga ugacucauac augaacaaaa cacugugcau gaccgaggaa     1800

aauaggaugc agaaggggaa ccguacuccc ugggaggcgu auggcggaga cccagcccgg     1860

uacgagcaga uccuucaaug ugcuagauau uuuccuaugg caaagaggaa acgcuuccug     1920

gaacagggca gcgauuuugu cgaccaguuc auucagcgac aauugaauga uacaagauac     1980

aucgccaagc ucgcuaaaga guaucuggcc gcagugugca ccaacguuca ggugacgaaa     2040

gggggaacca cagcgaugcu acggcaucac uggggucuga auaggcuccu gggcgaagac     2100

gaugggcaga aggagcgcgg agaccaucgu caccacgccc uugaugcugc cgucauagca     2160

gugacuagca gaucuuugua ccaacggcug gcuaggauug cccaucagcg cggccacgca     2220

cccucccagc gagguuuuua caucgaccca cccuggcagg gguaucaagc cgauuuagcu     2280

cagacccucg cgggcaucgu aguuagccau gccacaaaca gaaagcugac uggagcacuu     2340

cacgaggaaa ccgcuuacgg ccacauuccu cauguggggu ucgucuaucg gaagacacug     2400

aauaaagacu uuaagcuagg agauaucgag gccauuuacg acccaucgcu ccaggaacug     2460

gugaaagccc acuuggagca ucacggcaac gauccgaaga gggcuuucgc agaagguaua     2520

caguguuauc auaaagacgg gcacaccccc aucaagcgcg ucagaauuag ggccaaacaa     2580

gugucugaga agggaacuuu uuccaucuca gaccgggcgg gccgugcuua caagcaccau     2640

aaaacgggua guaaucacca uguugagauc cugcgccacc gagccacagg caagauugaa     2700

ccuguguauu uaaccacuaa agaggcaacc gaaagagcca ggcgaggggg agagccagua     2760

auacagcgcg aucauggccc cgaaugggag uucaucauua gccucugcau caacgacaug     2820

gucagacugg gggagggagg ccagguggcu uacuuuaggg uucagaagau ugaagcaaca     2880

aauaaccggg ugacccuucg aucccaccug gccgcgacuu uggaugacga ggauaugaaa     2940

gucacaacgu cugugccuaa gcucgcugua augccagguu uccaaaaggu gcugguuacc     3000

gccgucgggg aagugcuaga gcugcacgac uga                                  3033


<210>  2591
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2591
augaagagaa uggccgcugu gggccugaug cccgcagacc cuaccgaggc caaagcgcuc       60

gaacuucugg auccauacgc uuugagggcc gcaggguuag accaggcccu gccgcuaaca      120

cacuucggac gggcucucuu ucaucugaac caacgccgag guuucaagag caaucguaaa      180

acugauaagg gcgacaacga guccgggaaa aucaaggaug caacgaagag acuugacgaa      240

gagauggcca uuaaaaaugc uaggaccuau ggagaauuuc ugcacaugcg gcgcacaucu      300

gccacugauc ccaagaacgu cccuaccguu agaacaaggu ugucaauagc gcggcgcgac      360

aaugcagaga aagaggaagc cggcuacgau uucuauccag accgaagaca ucucgcugag      420

gaauuugaua agcuguggaa agcccaggca gguuacaguc ccgacgugcu gaccgaugag      480

cucagggacg agaucagcgu aauuaucuuc caccagcggc cuuuaaagac uccagaagug      540

gggcugugcu uguuuuccgg aucucauggc guccccaaga aagacaagcg uauuccuucg      600

gcucacccac uuaaccagcg cagaauccug uucgagacaa uaaauaaccu aaggguggcc      660

gcgcggggcg aaccggcccg agggcucacc cgcgaugaga gagacgcaau cguucacgcu      720

cuggauaaua aagcccauac gaagucacuu agcggaauga guaugaaacu gaaggcuuug      780

gguaaaguga uuaagcucag gcccgaacaa uccuuuacuc ugcagaccgc caaccgggag      840

acaaucgcau gugacccugu ccgcgccucu uuaagccacc cagauagauu cggccccagg      900

ugguccaugc uggacgcuga ugcgcagugg gacgugguac agcguauucg ggcaguucaa      960

ucagaggccg aacaugaugc ucucgugaag uggcuuaugg ccacucacgg gcuggaccga     1020

gaucacgcag agaguaccgc caaagcuccu uugccagaag gauauggcag acuaggucug     1080

acagccacca agaggauccu cgcagcgcug gaggcugacg ucauacccua cagcgacgcc     1140

gugaaagcau gcggguggca ucacucugau ggacgcacgg gcgaagugcu uacugagcug     1200

ccguauuacg gccagauuuu ggaccggcau gucaucccug ggacauacaa ugaguccgau     1260

gacgauguua ccagauaugg aaggauuacu aaccccacag ugcacaucgg ucucaaucag     1320

cugcgccgau uaguaaacaa gaucauuacc guguacggca aaccagacga aauagucgug     1380

gagcuggccc gggaucuaaa gcucucagaa gaccagaagc gugauguuca aagagacauc     1440

aaaaagaaua cggaugcugc cauugcacgc ggggcuaaac ugagcgagau gggagugccu     1500

gacaccggcg ccaacaggau gguccuucgg uugugggaaa gucuggguga ugacgugaug     1560

acaagaaacu gucccuauuc ugggaagagg aucuccgcga cuaugcucuu ugacggcagc     1620

ugcgauguag accauauacu gccauacucg cgaacccuug augacggauu cgccaaucgc     1680

acacuguguu ugaaagaguu uaaccggcag aagacugaua agacccccug ggaggcuugg     1740

ggcgacaccc cugcaugggc cacaauugaa gcuaaucuca aaaaccugcc agccaauaag     1800

gcauggagau ucgcgccgga ugccauggag agguucgaag gggagaacga cuuuacuucu     1860

cgugcuuuaa aagaugccca guaucugucc cgcaucgcac ggacguaccu agacgcucuc     1920

uaugauggag ccgacgguaa gucacacguu uggguggucc ccggcagacu gacagagaug     1980

cuuaggcgac acuggggguu gaauagucug cucccugaua aagacggagc agugaaggcc     2040

aagaaccgga ccgaccaucg ccaccaugcu aucgaugcgg ccgucguggc agcuacugac     2100

agaagccuga uucaaaggau cuccaaaaug gcccggaagg augaaguuaa uggcgccgag     2160

gaaguggcuc gaucuguacc acccccuugg gaggguuuca gaaccgacau ugaaacacag     2220

uuagaucgca uaaucauuag ccacagggca gaccacgggc guaucgaucc agccgcgcgg     2280

aaagacggcc augauucaac caguggacag cugcacaacg acacugcuua cggccuuggg     2340

gagggaaauu ccgucgugag cagaaagccc uugcucucuc ugaaaucaaa cgauaucgag     2400

guuacaacga agggugccaa uauuagggac guggaccuac agcgccaucu ggaacgaaua     2460

accagaggcc ucgaugggaa agcacuggag gccgcucuug cagaauuugc cgcgacugcu     2520

aaguugccgg acggaacaga uaacccuuac uauggccugc ggaggguccg cuuagaggaa     2580

acccuccaac agggugcccg uguggaggua aaggacagaa augggaaacc auucaaggca     2640

uacaaaggcg auaguaacca cugcuaugag aucuggcgga ugcccgacgg aaaggugguu     2700

ccucagguca uuacaacuua cgaagcccau cagaucgaug uggagaaaaa gccccaccca     2760

gcugccaaga ggcugcuucg cauuuuuaaa cgagacaugg uggcaaucga acaagaugac     2820

aaggcuauaa ucggcuaugu ccagagcaug cacguugcca acgggcuguu cauugcgccu     2880

cauaccgagg ccaaugcaga ugcuagaucc cgggacaaau cugauucguu uagguugauc     2940

cagaugagcg ccggaccccu cgugaaggcu aacgcacgca gaguguacgu agacgaaaug     3000

ggcaggcugc gggacccagg ugccccgucc uga                                  3033


<210>  2592
<211>  3039
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2592
auggccaaca ugagauacag gcuggcucuc gaccuuggca gcaccucccu ggggugggca       60

auggugcggu uggaugccca gaaugagaca ugcgcgguca ucaaggcugg aguucgcauu      120

uucucugacg gucgaaaccc caaagauggc ucaaguuuag ccgugagccg uagagaagca      180

agggccaugc ggcgcagaag ggaccggcug cuaaagcgca aaacucgaau gauggcuacg      240

cucaugaagc acggguuuuu cccucaagau gaggcagcca gacaggcucu gguaaauaaa      300

gaaccauuug cccuuagggc gaagggacug gaccaugcau ugcucccgga ggaguucgcc      360

cgggcucugu uucacauaaa ccagcgucgc ggcuucaagu ccaauagaaa aaccgauaag      420

aaagagacag acucuucggc ccugaagaaa gcaaucgguc agcucaggga ggcuaugcaa      480

gccucagggu gucggacugu gggagaaugg uuaugcgcgc gacaucagca gggccugagc      540

guccgcgcca gauauaggga gaaccggauu accaaggaug acggcaagac aaaaaucgaa      600

aagaguuacg aucuuuauau ugaccgcgca augaucgagg cugaguuuga ugccuugugg      660

gcuaaacagu ccgaacugaa ucccgagcua uuccacgaaa ccgccagagc agagcucaag      720

gacugucugc uucaucaaag gccucugaaa ccagugaagc ccgggcguug cacuuugcuc      780

ccugacgaag aacgggcccc acuggcuuua cccucucagc agcgauuuag aauauaccag      840

gagguuaaca aucugaggcu ccuucgcgaa ggacugaacg aggaaccuuu gacacuaacc      900

caacgggauc agcugaucca ggcgcucgag acgaauccaa aaagaucauu cacucaaauu      960

cagaagcugc uugguuuuuc cggccaguuc aaccugcagg acgauaagag gaccgaauug     1020

aaagggaaua gcacaagugc aaugcucagc aagaaagagu uuuucggauc ucaauggcug     1080

uccuucgacg agacucacca ggaugaaauc gugaugcagu uagucucaga ggaaaacgag     1140

gcccgccuga uugcuuggcu acaggccgaa accggcgugg acgagaucac agcagagcga     1200

auagccaaua ccccgcucgc ugaagguuau gggagccuga gugccaaggc acuugagaaa     1260

auuuugcccg cgcugcgggc ugauguaauc acguacgaca aggccguucu cgcagccgga     1320

uuugaucacc auucucacau uucccaugcu gccacuggcg aaauccugcc ugaccuucca     1380

uauuacggcc aauaccugca gcgucacgug ggguucggaa cagguaagcc cgaggauccu     1440

gcagagaaaa gauauggcaa gaucgcuaac ccaaccgucc acauuggguu gaaccaggug     1500

aggacugugg ucaaugcccu cauaaaacgc uacggacauc ccagcgaagu ugugguagag     1560

cuggcgcggg acuuaaagca gucgcaagaa cagagaaaag aggaacaggc ccagcaagcu     1620

aagaaccaga ggcgaaauga gcgcaugcgg caggacaucg caggccugcu aaacacaucu     1680

accgagagag ugagggcccu cgaucugcaa aagaugauuc uuugggaaga guuguccuuu     1740

aaugucgcug accgucgcug uccguauuca ggugugcaga ucagugccca gaugcuguuc     1800

agcgaucagg uugaaauaga gcacauucuc ccuuuuuccc aaacgcugga cgauucuuua     1860

aacaauaaaa ccguggcaau gcggcaggcg aacagaauca aggggaauag gacacccgcc     1920

gaagcugccg cagacuucgc ugcccagggc uggccagucg aggauaugcu gcaucgagca     1980

agccuuaugc cuaaaggaaa gcgguaccgc uuugcccagg acggcauggc ucaaugguug     2040

agagaggaua aaggguuccu cgcgagggcc cugaacgaca cucggcaccu aucacgagug     2100

gcaagagaau aucugagucu cguaugcccc cagggaaccc gcguuauccc agguaggaug     2160

acagcuaugc ugcgugccaa guuuggccuu aaugauauuu uggggcugaa cggagagaag     2220

aaucggaacg accauagaca ccacgccgug gaugcuugug ucaucgcagu gacugaccag     2280

uccauguuac agaaauucgc caaggcgagc gcuucugcca gggaaaaaca acucaauaag     2340

cuggucgagu caaugccccu uccuugggac aguuaccgcg aucaugugca gcgagcaguu     2400

gaaagaauuu gggugagcca caaaccagac cauggccacg agggugccau gcacaacgau     2460

accgcuuaug ggcuguuggg cggaggccau guacaggucc ggaagaggau ggacggggug     2520

cgcacacgug agaccgcagc ccucaaaguu auagaauuug cggauagaca agcucacccg     2580

cggcauggac ugcuacccaa cggugagccu augccauaca agggcuacaa gggggacucc     2640

aauuauugca uggaaaucgu gaggaacgau aaaggaaagu gggagggcga ggucgugucu     2700

acuuucgaag ccuaccagcu gguacgccag cacggucucg cacgacugag acaucccgcc     2760

cuuucgguga gcgggcggcc uuugguuaug aggcuguuac gcaaugacac gcucagaguc     2820

auucacaacg gcgagcagag gacagugcgg aucuguaaaa ugaccgcuga aggagugauc     2880

gcccuggcag auacuaccga guccaauguc gacgcucgua cacgcaccaa ggaucuuuca     2940

uauauuuuua aaacugccgg cagucugcaa aagucuaagg ggcgaagagu ugcgauaagc     3000

ccauccggag aaaugaaaga ccccggcuuc uucgauuga                            3039


<210>  2593
<211>  3051
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2593
auggaggugu ucgucgacgu uagcgccaga cagcugggca ucgaucucgg gauuggaacc       60

ugcgguuggg cacuucuggc ugaagacucc uugauagccu uaggcacaag gacuuuugau      120

gugcccgaga cggacaagga acggaucccu accaaccaaa uucgccgaca ggcgcguggg      180

augagaaggg uaaucgaucg gcgcagacag aggaugaaug cugugcggcg ccugcuagcc      240

gaccacggac uccuggcaga uggcgguaaa cuugggcugg ccacaguccg auuggaccca      300

uggcaggcua gagcagaggg acucgauagg cugcugucug ccggcgaacu cgcuguggcc      360

uuaggccaua uugcgaagca ccggggguuc cguucaaaca guaaacgcga cagaggagca      420

aaugccccga aggagagcuc cgcuaugcug ggugccaucg cacaaacugg cgagaggcuu      480

cagcauuggc ggaccguugg ggaaauguuu uggaaagaug aguucuacca gacacgacgc      540

agaaacaggg acggagauua uucucggacc gugcagcgcu uggaccugga aacugagguc      600

cacagacuac ucgcugccca aaggcgucug ggcaaucccc uugcgacauu ugaccuggag      660

cagucguuca uagauguggc cuuuuuccag cggccuuugg cagacucaga ugacaaggua      720

gguccauguc ccuuugaacc uagcgagaag cgagcugcca gacauagucc auccuucgaa      780

agguuucgcc uccugucucg guuagcugcc cugagacuca gcuccggggc aggagagagg      840

ccccuuucag augccgaguu cgcugcggca ugcgccgacu uuggccgccu gcagggcuuc      900

aguuucaaac gauugcggca acaccuaaag cuggauccua gcauccguuu ugcugggauu      960

gccccagacg aggagggaaa aagagauguu guggcaaggu cuggugaagc caccccgggc     1020

uccgcugccc uccgccacug ucugggggac acggucuggg auaacaugcu uggaacucgg     1080

cccgcacugu uggacgcgau cgcugccauu cucaccuuca gagacgaugc agacaggauc     1140

gccgagggcc uggcugguuu agggauagag gaaccuauuc uggaugcccu aauggacggc     1200

gugcgaaaug gaucauuugg caaguucaaa gggccaggac auaucagcgc aaaggcuugc     1260

cgcgcccucc ugccccaccu ugcgcggggu agaguguaca gugaugccug ugcugacgca     1320

ggcuaugauc auucuaggca gacagaaacu aacuuggacg auauucguaa uccugucgcc     1380

cgcaaggcuc ugggggaggc ccucaaacag aucaagauca uuauacacac ccacggacgg     1440

ccaacagcaa uccauguuga gauggcgaga gacauuggca aauccgccga ugaaagggac     1500

gagaucaaga aagguauaga uaagcgaaac agcgaaaaag accgggcucg ccaacaguuu     1560

cacgagaugg uggggacccc cgccgcaggc gcugaagacc ugcuuagauu cgagcugugg     1620

aaggagcaga auggauggug cuuguacacg ggcagggaaa uuccgaucga ugcccucgua     1680

ucgucugaca accggaucca gguggaucau auucugccuu ggucccgauc aggggacgau     1740

aguuauauca auaagacuuu auguuuuaca gaggcaaacc aacacaaaaa gggaaugacc     1800

cccugggaau ggcugcauga cgauccagag agaugggaga aauucgcccg cacugucgaa     1860

gguugcaaga caaugaaagg caggaagaag cguaaucuac uccugcggga cagcuccaga     1920

cuugagaaag aauuuagguc ucgcaacuug aacgauaccc gauacgcuug uagacugcuc     1980

cuggggauuu uaaagcacac guauccugac auagugguug cggcccggcc cggaccacug     2040

accgauaggc uucgccgugc auggggcuug cagagccuca aaaagcacga ggacgguaca     2100

agaaaagaug acgaccggca ucacgcucug gaugccgccg uggucgcugc aacuucacag     2160

aguaugcuac agaggcugac cacacucuuc caaaaggccg aacgcgaagg gggccgaaga     2220

gacuuugugg gacuggagcc cccuuggcca ggcuuccggg cggaucuuga agcuaaguug     2280

gaggggguag uuguguccag ggccgaacgc agaagggcac ggggagccgg ccauaaagac     2340

acuauccgug cuauugguga gggggaugca ggaccggucg uguacgaacg caagcccguc     2400

gccgcgcuga ccgagaaaga cuuagaucga gugaaggacc cugagagaaa uaaaguucuc     2460

aucgaaaacc ugaggcagug gaucgcugcc ggcaagccaa aggaucccga caaagcaccu     2520

cggagcccaa agggugaucc caugacaaaa gugaaacuug uaaccacuga cgacguccgc     2580

auucugguga gaggcgggac ggccgauagg ggagacaugg cucggguuga uguguuucga     2640

caggccgacc cgaagggcag auggcgcuuc uucuuggucc cuguguaccc acaccaggua     2700

uuugauccca cagacuggcc ucaaccccca aauagggcag ugguugcuua uaagccugag     2760

aaagaaugga ccgucauggg gcccgaucac gaguuccucu ggucucugua cccauuuuca     2820

uggauagccg uggaaaaggc ggacggaacu cggaucgagg gcuauuuccg ugguaugcau     2880

agaaguaccg gggccauuag caucuccccg caccauucua agucgcuagc aagcacagga     2940

auuggcgcua aaacccuggc cuccuuuagg aaguucgcua uggaucgcau ggguaaccuc     3000

ucagagaucg aacgggaggu gcgaacuugg cacggcgucg caugcacaug a              3051


<210>  2594
<211>  3054
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2594
augagagugg acagccuggg gaggugccuc uccgaugagg aaugggcccg ggucaucuac       60

caccuuugua agcaucgcgg cuuccacugg auuucucgag cugaggcaaa acagaccgaa      120

ggagacucaa agagugaggg uggcaaaguu aagcaagggc uggccggaac agcgaaauug      180

auggaugaaa agggcuaucg uagcgcugcc gagauggugu uagcagaguu ucccgaagcc      240

cagagaaaca agcaggguga guacaggaaa gcucuguccc gggaacuacu cgcaaaggag      300

cuggcccuuc uguucgcucg ccagagagua uuugggaaua ggcaugccac ugacacguug      360

gagaccgcga uacucggaaa cucugauaaa aagucgggcc uguucuggca acagaaaccu      420

gcacuggccg gcgcugaccu cuuaaagaug cuggggacau gcacuuuuga aaagaaugag      480

uaucgggccc caaaagcauc auucaccgcu gaacgccacg uguggcuuac acgauugaac      540

aaucugagaa ucgucaccga uggaauuacu aggcagcuaa cagagcagga acggcaacuc      600

gugcugccgc uucccuaccg ucaggccggu gaccugaccu auaagcaguu gggcauggcg      660

cucacgaaag ccgggcuguu agaggcagga uguuuuaagu ucaucggccu ggcuuacccu      720

acugaggccc agaaagcuga agguaagacc aaagauccag agagcgccac acucguuaag      780

auucccgcau ggcaagaacu ucgccagacu cugaacaagg ccaguuugaa ugacgagugg      840

gaaaaacuau ccggggaugc ucugugcgga cagccugaga ucuuugacaa gauagcgugg      900

auccucucug uguauaaaga ugacgacgag gucagaaccg aacuggcaaa acuugugcug      960

ccaaaccccg agggcuugac agaagcccuc cugaauauua gguucgauaa guuucaugcu     1020

uuaagccuga aggcccuacg ggcaaucgua ccucacaugg agaagggccu ccgauacgac     1080

gaagccugug uucaagcugg guaccaccau ucccagcugc acaaaauugg agagggugag     1140

cauaaguauc uuccaccguu guacgauggc cgcgacgggg auggaagaau gaucuucgcc     1200

gaagacgcag auauacccag gaacccugug guccugcggu cacucaauca ggcgcgcaaa     1260

guggugaacg cuauuaucag aagguauggc aguccaagcg ccguccacau ugagauggca     1320

cgugaccugu cucggcccuu ugaugaacga aauaagauca aaaaggacca ggaugaguac     1380

caagccagaa accagaggga ccgcgagcag uucaucgaac uuuucgguca cccuccaaag     1440

gggcuggagu uugaaaaaug gcgguuguau agagagcaga auggccaaug cgcuuacucc     1500

cagaagcccc ucgccucaag cggagacguu gcagaaauuu uccuggaugg caguaccgag     1560

auagaccaug cuuuaccgua uucuaggucc uuugaugaca gcaaaaacaa caaggugcug     1620

guacuaacgc gcgagaaucg agauaaaggg aaccggacuc cuuacgaaua ccucggaggu     1680

gccgagaaua gugaacguug gcagagauuc uuuucuuucg uggaguccaa caaggcguau     1740

aggcuggcca agcgcacacg gcuuuugaga aaagacuuug gcgcuaagga aucagaggag     1800

uucagggaac gaaaucugaa cgauacccgc uacgucuguc gguuuuucaa aaauuaugug     1860

gagcagcacc ucaagcuggc agaagggagu gaggccaaaa gaugcguugu ggucagcgga     1920

caauuaacua acuuucugag gacacguugg ggccuuuuga agauccgcuc cgacucugau     1980

cggcaucacg cucucgacgc cgcaguggua gcggccugua gccauucaau gguucagaga     2040

cugaguaauu acuccaggac ccgagaacua gaacaggugc gggaugguuu cgucaacauu     2100

gagacggggg agaucgugaa ucccgcuaug caccagcaac ugcgcgagca cuuuccagac     2160

ccuuggcccu auuucagaca ugaacucgag gccaggcuga aaauagauag cccagcacuu     2220

uugcgggagg aaauggcucg acugucuuca uacaccgagg aagaguuagc cgcacucaag     2280

ccccuguucg ucaguagagc cccucagcgc aggaacggcg gagcugcgca caaggacaca     2340

auuuauggcc agccagaaaa acuuaagaaa aaugggagcg ugucccaaaa gguugcccug     2400

gcaucuuugg cucucaaaga caucgaucug cuagccgacc cgcaucguaa cgccaagcug     2460

uacggagcua uccgggagag acucgaggca cacgguggca agggggauaa agccuuuccc     2520

gcgacuaauc cucugaggaa gccagacaaa gaaggaaacc ccaccggccc uauugugcgc     2580

acaguaacua aggucaucga uaaacuuucg gguauuccag ugcgaggggg cauagcuaag     2640

aacgacacca uguugagagu ugauguguuc acaaaggccg gacgguuuca ccuggucccc     2700

guguacguac aucacagggu gaccggcuua ccgaaucagg caaucacucg cgggaaagac     2760

gagaaccagu gggccauuau cgaugacacg uucgaauggc guuuuagccu cuauccuaau     2820

gaucugguuc agaucagacu uaaggacgag acacaucugg gauacuaugc uuccaccgau     2880

cggucaacuu cgucuauuag cauaugggca cacgacagga accaauccgu cggcaaagac     2940

ggucuaaucc gcucaauugg ggugaccaca gccaaggaua ucgaaaaauu ccauguggac     3000

acccucggaa augucuaccc agcgcccccu gagaagcgaa gaggccuggc uuga           3054


<210>  2595
<211>  3066
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2595
augagaaggc ggcgcgaccg auucaagcag cgucaagccg cucugcucaa acaccuugca       60

ggcguggggc uguuucccgc cgaugaggcg gcuagaaggg aauuggccgg auuagacccu      120

uacgagcugc gggcacgcgc ccuacaugaa gcucucccac ugaaccacau cgguagagca      180

cuuuuccauc ugaaucagag gcggggcuuu aagagcaacc gcaaaaccga ucgagggaag      240

gccgacgaug agggaaaaau uagauugggc gucgcuaggc uccaggaagc caugcacgag      300

aaggguuccg cgacacuggg ggaguuccug cauggacggc gugcagccgc uacugacccg      360

aaucagauac ccucuguucg cacgagacuc agggccgaau caggcgaggg cgcacgaggg      420

gauggauaug acuuuuaccc ugaucggcgc auguuagaag acgaguucga ggcuaucugg      480

gaaagucaac aggugcacca cccagccgua cugaccgaug agauuaagca gagauuuuuc      540

gaagugaucu uuuaucagag gccccuuaaa caaccugaga uugguacaug cacuuugguc      600

gcgggcgaac ggcgccugcc aaaggcccau ccccuauucc agaaaagaag gcuccuggag      660

gagcuuaacg cacugcguau cguguggccu gggguucagg cugaauugcu cagccgggag      720

cagcgagacc uggccuacuu aaagcugaaa gauaagagag cugugagguu ugaaucccuc      780

cgcaaggccc uuaaacugga cccagacgca cgguucaaua aggagucuga aaacagaucg      840

gaguugaaag gagaugaggu cgccgcugaa cuaggcgcga agucaagguu uggugggcgc      900

uggggacaca ccgcaccggc cgaccaaugg gagauaguga gccgaaucca ggcucuggaa      960

agugaugacg augaggccca guucugugua uggcucuccg aacgguaugg ccuggacucu     1020

gagcaagcac gugccguugc ugccgcaaga cuuccccagg gcuucgggag guuuggacug     1080

acagcgacca cucgcuugau uaaagcucuc caggaugagg ugaucgucua cagcgaagcc     1140

gugcagaaga caggucuggg ccaucacucc gacuuccgga auggggaggu guuagcagaa     1200

cugccuuauu acggaauugc ccuagagaga cacaucaugc caggcaccgg ugaucccggg     1260

gacucagaug agaugagggu cggccgaguu acgaacccua cugugcauau aggacucaau     1320

caacugcgcc ggguagugaa cagacuuauu aaaagguacg gcccaccccg ucagaucgcu     1380

gucgaauugg cccgcgagcu gaagcucgac gaccagcaca agcgggaaau uaaugggaga     1440

aacaggcaga aucgagaggc agcugaacgg cgcagucaaa aacuggugga uaucggaauc     1500

gccgacaagg gugcgaacag agcccuucug aaauuguggg aggagcucaa uccggauaac     1560

gcucuggaca ggcggugcgu uuauaccggc cgacagauua gcauagaaau guuauuuucu     1620

ggggcagugg aggucgauca uauccugccu uucgccgcua cacuagacga uuccaacgcc     1680

aauaagcucc ugugucuuag agaagcaaac cgcaauaaaa ggcgucgguc acccuuugag     1740

gcguucggac agggcgccga cugggaagag auugcugcca gagcagcuag guugccacgc     1800

gagaagcgau ggagauuuga accugaugcc auggcacggu ucgacacuga ggaaaaguuu     1860

cuggccaggc agcucgugga uacccaauac cugagccgcu uagcucguga guaucugggu     1920

guacuuuucc ccgacaaagg ggaaggcagu ggaagaguuu gggugucucc aggccgguug     1980

acagagaugc ucaggcgcuc cugggggcug aaccaccuac ugcccgauca uaaucucgga     2040

gguggcgcgg accagccuaa gaaccgaaga gaccaccggc accaugccau cgaugcaaua     2100

gucguggcug ucaccgacag gcaucugcuu caggccauug cccgcgaggc ugggcagaga     2160

ggacacgaag cagccaauag gaugacggcg gauuugccag ugccguggcc cgacuuugau     2220

cgggacguug gccaagugau ccgucaggua gucgugagcc accgcgcuga ucacgguacu     2280

guuucgaaag ccggggcagc caagggccag gacucuacag cuggacgacu gcauaacgau     2340

accgcauacg gcuuaacugg gcaaacagac gagaaaggaa auaccaucgu ggucagaagg     2400

gugccucuca cggcccugaa gaagccaacc uccauugaag cgguacggga ugacgagcuu     2460

cgcaaagcuc ugucagugug gacaagaggc uaugauggua aggccuucga ggcaagguug     2520

agucgguuuc ccgaacucgg gccucugcag uuccgaggaa ucagacgcgu uagggucgug     2580

gagccacuaa cugugauucc cauacgggac agcuccggcc gugucuacaa agguuauaag     2640

ggcgacucua acuacagaua cgauguuugg gaacugaaag acgggaagug gguggccgag     2700

gugguaagca uguucgaugc ucaccaguca aguuggaccu ccccggucag gcgcgaaaau     2760

ccuacagccc ggaaggugcu cagccugcag caaaacgaca ucguugcaau ugagccagag     2820

ggaaaagaac gagagcuuau gagaguggcu caguugaggc agggcgggca gaucacucug     2880

gccgcgugcc augaagccgg agauuuaaag cgccgggacg cagagucuaa ugaagucgau     2940

cccuuuaaau auuucucacc uaccgcuggc ggucucaaga gagugcgugc caggcaaguu     3000

cggguggacg agcuggggcg cguacuugau cccggaaguc gagacagaga gaggggcgcu     3060

gcauga                                                                3066


<210>  2596
<211>  3066
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2596
augaaguaca cccugggccu cgacgugggg aucgccagcg ucggaugggc uguuauugau       60

aaagacaaca auaagauaau cgaucuuggu gugagaugcu ucgacaaagc agaggaaucc      120

aagacaggcg agucucuggc cacugcgagg cggauugcuc gcgggaugcg acguagaauc      180

ucaaggcgga gucagcgcuu gagauuagug aaaaagcugu uuguacaaua ugaaauuauc      240

aaggauagcu ccgaguucaa caggauauuu gacacgucuc gggauggaug gaaagacccc      300

ugggaacuac gcuacaaugc ccucucgcga auccugaagc cuuaugagcu uguccaggug      360

cugacccaca uuacaaaaag aaggggcuuc aagucaaacc ggaaagagga uuugagcacu      420

accaaggaag guguugugau cacaaguauu aagaauaacu ccgagaugcu ccguaccaaa      480

aauuaccgca cuaucgggga aaugauauuu auggagacac cagagaacuc uaauaagaga      540

aacaaagucg acgaauauau ucauaccauc gcaagggagg aucugcugaa ugaaauuaag      600

uacaucuuca gcauccagcg gaaacucgga uccccguuug ugacggagaa guuagaacac      660

gacuuccuga acauuuggga guuucagcga cccuucgccu caggcgauag uauacuuagc      720

aaaguaggca aguguacuuu gcugaaggag gaacuacgcg cuccuaccuc uugcuauaca      780

uccgaguacu uugggcuccu gcaaucaauc aauaaccuug uucuggugga agacaauaac      840

acuuugaccc ucaacaauga ccagagagca aaaauuaucg aguacgccca uuucaagaac      900

gaaauaaaau auagcgagau uaggaagcug uuagauaucg agccagaaau ccuguuuaaa      960

gcucacaauc ucacacauaa gaaccccagu ggaaauaacg agucuaagaa auucuacgaa     1020

augaaguccu aucacaaacu uaagagcacc cugccuacgg acauuugggg uaaauugcac     1080

ucgaauaagg agucucuaga uaaccuguuc uacugucuca cugucuauaa gaaugacaac     1140

gaaaucaaag auuaccugca ggccaauaac cuugacuauc ugauugagua cauagcgaag     1200

uugccaacau uuaauaaauu caagcaucuc ucccuggugg caaugaaacg gaucauuccc     1260

uuuauggaga agggcuacaa guauucagau gccugcaaca uggcugaauu agacuucacc     1320

gggaguagca aacuggagaa guguaauaaa cuaacugugg aaccuaucau cgagaacguc     1380

acaaacccag uugugauucg cgcccucacc caggcaagaa agguaauaaa ugcuaucauu     1440

caaaaauacg gacugccgua uauggugaac aucgagcuug ccagggaagc gggcaugacg     1500

cgucaggauc gggacaauuu gaagaaagag cacgaaaaca aucgaaaggc cagagagaag     1560

auuuccgauc ugaucaggca gaacggucgc gucgcaucug ggcucgacau acugaaaugg     1620

cggcuuuggg aagaccaggg aggcagaugc gcuuacagcg gcaagcccau cccugugugu     1680

gaucuguuga augacucacu cacccaaauu gaucauaucu auccauacag uagguccaug     1740

gacgauagcu auaugaacaa aguucuggug uuaacagacg agaaucagaa caagcgcucu     1800

uacacucccu acgaggucug ggggucaacc gaaaaauggg aggauuuuga agcccgaauu     1860

uauaguaugc aucugccuca gagcaaggag aagcggcuac ucaaucguaa cuucaucaca     1920

aaagaccugg auuccuuuau aucuagaaau cuuaacgaca cuagguacau uucgcgcuuc     1980

uugaagaauu auaucgaaag cuaccugcaa uuuuccaacg auucaccaaa aaguugcgug     2040

guauguguua acggacagug caccgcucag cuccggucua gauggggucu gaauaagaac     2100

agggaggaga gcgacuuaca ccacgcccug gaugcagccg ugauugcuug ugcggaccga     2160

aaaaucauaa aggaaaucac aaauuauuac aacgagcgcg aaaaucacaa cuauaagguc     2220

aaauaccccc uuccguggca uuccuuccgg caggacuuga uggagacccu cgcaggcgug     2280

uuuauuucaa gagccccuag gcguaagauc acugggcccg cucacgauga aacgauucgc     2340

agcccaaaac auuucaauaa gggacugaca ucugucaaaa ucccucuaac cacugugacc     2400

cuggagaagc ucgagacaau gguuaagaac accaaaggcg guauauccga caaggccgug     2460

uacaauguac ugaaaaaccg gcuuauugaa cacaauaaca agcccuugaa agcauuugcc     2520

gagaagaucu auaaaccacu gaagaauggg acuaacggcg cuaucauuag aaguaucagg     2580

gucgaaacac ccagcuacac gggaguguuc cgaaaugagg gcaaggggau uucugauaac     2640

uccuuaaugg uucgggugga cgucuucaaa aagaaagaua aguauuaccu cgugccuaua     2700

uauguagccc acaugaucaa aaaggagcug ccaucaaagg caauugugcc gcuuaaaccc     2760

gaaagucaau gggagcugau cgacagcacc caugaauuuu uguucucgcu cuaccagaac     2820

gauuaucugg uuauuaagac uaaaaaggga aucacagagg guuacuaccg cuccugccac     2880

agaggcaccg ggucucuaag ccugaugccu cauuuugcga auaacaaaaa ugucaagaua     2940

gacaucggag ugaggacagc uauuucaauc gaaaaguaua acguggauau ucucggcaau     3000

aaaaguaucg ucaaggguga gccacggcga gggauggaga aauacaacuc cuucaagucu     3060

aauuga                                                                3066


<210>  2597
<211>  3072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2597
augaagaucc ugggcuucga cauuggggug gccagcauag gaugggcuuu ugucgagggu       60

gaugaaauca gagacugcgg cguuagaauu uucaccaaag cagagaaccc caagacaggg      120

gaaucccucg cccuuccucg gcgcgaggcg cgaggagugc guagaaggcu ggcucggcgc      180

agaguaaggu ugaauacuau caaaaagauu aucuguaaag aauuugauuu acggcugucu      240

gacuacauag ccgaugacgg cgagcuacca aaggcauauc agacgaacaa ggauaccaaa      300

ucaccguacg agcuccgcua uaaggcccug aaugaacgac uuaguaaaga ggaacuggcu      360

agagugaucu ugcacauugc aaagcauagg gguuacggga acaaacacgc caaggacgga      420

gaugacaagg auagcggcaa agucaagaaa gcuaucggcg agaauaagac acuccuggag      480

caaaaagggu augaauccgu gggagaguac ugcuuccggg aauuuuucaa gucuauuaaa      540

gacaaggaug gugagacuca uaacaucccc ucgcagaaua uacguaacaa gaaaggcaau      600

uaugaccgcu guguuucaca gaagcagcug gaagacgagc ucaaauuaau uuuugccaag      660

caaagaaaac ugggguucaa caaggaguuu gaagaugcga uccuugaggu ggcauucuac      720

cagaggccuu ugaaggacuu ugaugacaaa auuggagauu gcaucuucuu ugacaaugaa      780

aagcgggccc caaaagagag caucagugcu auugaauuug ucgcccugac ccgaauaauc      840

aacacacuaa aguaccucuc caaaaccacu ggcgaggugu augagaagga aauuaucaau      900

aagauauggc agaaaauucu ggagaacggu aagaugacau acgcagaacu ucgcaaaauc      960

cuggcuuugg auucuagcgu auucuuuaag gacaaagauc ucgacuauac ccuggggauc     1020

aaggaggccg aaaagauuaa auucaucgag uuuaagaaau uaauuaaguu caaaaaggcg     1080

cugggauccu uugauggccu cucaagagag auacuugaca auaucgccau gcagauugca     1140

aucacgaaag acaggcaacg gcuguuggaa aagcuagagg uuuucccccu gaacaaggaa     1200

cagaaagaga accucaguca gcugaauuuu agcaaguaca ucgaucuuuc ucugaaggcu     1260

uugaaccaga uucucccuuu caugcgcgag ggcagaaggu augacgaagc caaggaugac     1320

gcuaaacugc aagagaaggc caagaauacu aaaaaggaag aguuuuuacc accccugaaa     1380

gaguucgauc uaaaccucac caauccugug gucgcacgug cccuggcuga auuucggaag     1440

gugcuuaacg cguugcugaa aaaguacggg aaggugcaca aaauacaucu cgaguucgca     1500

cgagacauga gacuggauuu ugacgaaagg gagcgccuua ucuccauuca gaaugauaac     1560

uucaauaagc ggcagcuggc cgaagacuuc uguaaagagc acggauugga accaucaaac     1620

gcuaauaucc ucaagauuaa acuguggcaa gagcagcagu ggcacugcau guauagcggu     1680

gaguuaaucg gcauaccgga ucugaaggac cccacaaagc uacaggucga ucauaucuac     1740

ccuuacagua gaucuuuuga cgacuccuau acuaacaaag uucucgugag ggccgaaugg     1800

aaucaagaga aggggaacca caccccauuc gaagcauuug gaggcgauaa aaauaagugg     1860

gacaaaauug uagcccuggc ugagaaacuu cccccuagca aaaaguugaa ccgcucggaa     1920

aagucuaaaa aggauaaacu gcucgacgag aaguucaacg auaaagagcu ggguuucuua     1980

gcccgaaaua ucguggacac aggguacauu gcacggcugg ucgcgaagua uaucgauaug     2040

uaccuugagu ucuugccacu cgaagaaaac gagaauaccu cccuggcucg uggcgagaag     2100

ggaaguaaaa agcauauagg cauugugaac gggagacuaa cgucagaagu uaggcacaau     2160

uggggacugg accccaaaga ucgcgacaac caccuccauc acgccaucga ugcaauugug     2220

aucgccuaua ugaaugacaa gcggauaaag gcuuuugccg auuucagaag gcugaaagag     2280

cuuaagauca aacgauacgg ucgcgaaauu acugaggacg aauaucagga gaagaaaaag     2340

gaguugagcu gugcaccguu ugacgauuuc cggaacagaa cacuggaaaa uaucgacaaa     2400

auuuuugucu ccaggccucc ccguaagcgc gcucggggca ccuuacauga ggaaacuuuc     2460

uacucuaucg augacaagaa acuccugaag gccuacgggg gaaaagaggg caugcagaga     2520

gcgcuuagcc uggguaagau aaggcagauu gggacaaaaa ucgugaagaa cggcaccaug     2580

guacgaguug auaucuuuaa ggacaaacgg ggacgcuucu auggcgugcc aauuuacacg     2640

auggauuucg ccuugggggu ccucccuaau aaggcugcag ugucaggaaa agacaagaac     2700

ggucugauca aagaaugggc cgagauggau aguggcuaug aguuuugcuu cucccuauuu     2760

aaggacgauc ugauucucgu ccaaagaaaa gaaaugcagg agcccgaacu guguuacuuc     2820

gugagcuuug acucuucaaa ugcucagaua gacguuagga gucacaacaa ucauuucagc     2880

gugcuuaacg agaaccagaa guugcuguuu aaguccgcca cccaagcaga aguaaucggg     2940

gagucuauug gaauccagaa uuuaaaaguc uucgagaagu ggcacguguc gccacucggc     3000

gaaguucgga aagcggaguu uagcccccga ggugaagcca agcugaaaac auccaagaag     3060

aggaaaaagu ga                                                         3072


<210>  2598
<211>  3072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2598
augaagaucu acagcuucga cauuggcgug gccuccauag ggugggcuuu uaucgagaac       60

gaaguccugc aggauugcgg aauuagaauc uucaccaaag cagagaaucc caagaacggu      120

gccucucucg cgcuuccuag gcgggaagcu cgcggcguuc gaaaacgucu ggccagaagg      180

cgggggcgcu ugaauacaau uaagcaauua cuguguaaag aguuuggacu acagcucgaa      240

gacuaucugu caaacgaugg cgagcuucca aaggcauaca cugccaguaa ggcuaauccg      300

cugaaaagcc ccuaugaguu gagaacgcuc gcacuguccc agaagcugga cucuaaagau      360

cucgugaggg uaaucuuaca cauagccaag caucgggguu acgggaacaa acacgcuaag      420

gacucgaagg auaccgaauc aggaaaagug aagaaagcca ucgaguucaa ucgcaagaca      480

cuggcggaaa aaggcuaucg aagcgucggc gaguaccuuu auagagaguu uuuccagcaa      540

aguaggauuu cccagaacaa uggggcaacu gaauuuauca acgugcggaa uaaggccgga      600

aacuacgagc auugcguugc ucaggacaug uugaaagaag aacuggaauc uauucuaaau      660

gcccagcguc aauucgguuu uagccucaag caggauuucg aguccaagcu gcuuaccaaa      720

aucuuugagc agcgcccucu gaagucauuc gcagacaaag ugggcaacug uugcuuuaua      780

gcuggggaaa agagagcccc aaaagauagu cccagcgcga uugaguucau cgccuugucu      840

aggacaauua auacccucau caaccugucc aaggaaucag gagagaucua caaccgggca      900

auuauauuac agaucgucga aagcacucug aagaaaggcg agauuaguua uaaggagcuc      960

cgagaaauca uacgccuuga cgagagacug agguucaaag auccucgguu ggacuacucu     1020

cgcgaaauua aggaggcuga aaaagugaag uuucuagagu ucaagcaacu gaaagguuuu     1080

aagaaagccc ucggggaguc cuucacaacc cuggcuauca gccagcuuga cgaaaucgcc     1140

gagcacauug cacugaucaa ggauagagua aaauugaaug aaaagcucga gucguaugcc     1200

cugacgaagg agcagaggga ggcuuuaucu aaccuguccu uuucagcgca uauuaaucua     1260

agucuccaag cacugaaaca gauacuucca uucaugcgug gagacaacgg cgagcagugu     1320

uuggauuacg acgaaagcgu ugagaaggcc ggccugaaag cucucuccaa ggauacucug     1380

aaagggcaga ugcuuccccc ucugaaugaa uuugagccau auuugucuaa cccggugguc     1440

gcccgggcac ucgccgagua ccgaaaggug cugaaugugu uacugaaaca auauggaagc     1500

ccccacaaga uccacauuga auacgcuaga gaggccaagc uaucaaguac cgaaaggcag     1560

aaauacgaga aggaacagaa agacaacuau gcagcgaauc aggcugccca uaagaaaugc     1620

caagagcucg gucuggagcc uuccagcaca aaccuuuuga agcugaagcu cuggcaggaa     1680

cagggcgggc uguguuuaua cucuggagca aaaaucacua ucucauaucu gcaggaucca     1740

accgcccuuc aagucgacca cauuuacccc uauagucgca gcuucgauga cuccuacaug     1800

aauaagugcu ugguucucac aaaagcuaac caggagaagg gcaaucggac cccuuuugaa     1860

gccuucggua cggauucugc aaaauggggc gugauaacua cacuggcuca gaagcuacca     1920

cauaagaaaa gaaggcgcau ccugaacacc gccuuuaaug acaaggaggc gggguucaaa     1980

cuccgaaacc ugaacgacac uucguauauu gcccggcuug uagcugauua cacagaaacc     2040

uacuuggagu uucugcccuu aguggagagc gaaaauacga cccucuccaa gggacagaaa     2100

ggcucaaaga agcacaucgc aauugucaac ggggugcuga caaguacuau gcguuauuac     2160

cuuggauucg ccucuaaaga cagagauaau caucugcacc acgcuuugga cgccguuauc     2220

auagguuuca ugaacgauag cgugaucaag gcauuuuccg acuucaaaaa gacccaagag     2280

acaucaaaag cggccuauua cgcucauacu cucagcaagc aggaauauaa aaagcagagg     2340

gccuuuauuc aacugccguc uggcgagaau uuccgcgucu ccgugcuaga gaagguagaa     2400

aguguuuuug ugagcaaacc uccccggaag agaguccgag gggcacugca cgaggcuacc     2460

uucuacucuc caaacgaucc uaaacuccug aagaauuaug gaggcacaaa agguguggaa     2520

agggcccuug cauuggggaa gaugcgcaag gucggcgcca aaaucguguc caacggagcu     2580

augguucggg uggacauuuu uagacauaag ucaaguggca aauucuacgg gguacccguc     2640

uacaccaugg auuuugcgcu gggaaucuua ccaaauaagg ccgugguugu ggguaaagac     2700

cagaacggcg ucauaaagga uuggcucgag auggacagcu cguaugaauu uauuuucucc     2760

cuguacaagg augacgugau ccuuguacag aaaaaggaga ugcagaggcc cgagcugugu     2820

uauuucgugu cuuuugacac uagcggggca ucaauuaaag uugcuaagca cgauaauaac     2880

uucaguacgc uaacagaaaa ucaaaaacuc cuguucucca acgccaccaa ggaggaaguc     2940

gugggaaagu cuaucggcau acagaauuug aaaguguuug agaaguggca ggucagcguu     3000

cugggugaag ugaaacguac ucaguccuac ccucgcgaga acaucucauu agccagcaag     3060

gcuaaaaagu ga                                                         3072


<210>  2599
<211>  3075
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2599
augaucagaa cccugggcau ugacauaggg aucgccagca uuggaugggc ugugaucgag       60

ggugaauaca cagauaaggg ccucgagaac aaagaaauug ucgcauccgg gguuagggug      120

uucacuaagg ccgagaaucc caaaaacaag gaaucucuug cgcugccucg gacguuggcu      180

cgcucagccc gacguagaaa ugcaaggaaa aagggacgga uccagcaagu aaagcacuau      240

uuaaguaaag cccugggccu agaccucgag ugcuuugugc agggugagaa gcuggcuacc      300

cuuuuccaga caagcaaaga uuuucugucc ccaugggaau ugcgcgagag agcacucuac      360

aggguccugg acaaggagga gcuggcccgg gugauacucc auaucgcuaa acgccgaggg      420

uaugaugaca uuacuuacgg aguugaggau aacgacucug gcaagaucaa gaaagccauu      480

gcggaaaauu cgaagagaau caaagaggaa caguguaaga ccauaggcga gaugauguau      540

aaauuauacu uccaaaaguc acugaacgug aggaauaaaa aggaaagcua uaaccggugc      600

gucgggcgua gugagcuucg cgaggaauug aagacaauuu uucagaucca gcaggagcug      660

aaauccccgu gggugaauga ggaacuaauu uacaagcucc ugggaaaccc cgaugcacaa      720

ucuaaacagg aaagagaggg ucuuaucuuc uaccagaggc cucugaaggg cuuuggggac      780

aaaaucggaa aguguagcca cauuaagaaa ggcgaaaauu ccccauaucg ggccugcaag      840

caugcucccu cagccgaaga guucguagca uugaccaaaa guauaaacuu ucucaagaau      900

cugacuaacc gacacgguuu auguuucagc caggaagaua ugugcguuua ccuggggaaa      960

auccuccaag aggcucagaa gaaugaaaag ggacuuacau auucuaaacu gaaguugcua     1020

cuggaccucc cuuccgauuu ugaguuccug ggccuugacu acucaggcaa aaacccagag     1080

aaggccgugu uucugagcuu gcccaguacc uucaaacuca acaagauuac gcaggaccgc     1140

aagacucaag auaaaaucgc gaauauacug ggggccaaca aggacuggga agcaauuuua     1200

aaagagcugg aaucucuaca gcucuccaag gagcagaucc agaccaucaa agaugcuaag     1260

cugaauuuca gcaaacauau uaaccuuucg uuggaggccc uguaucaccu ccugccucuu     1320

augagagaag gaaagaggua cgacgagggu gucgaaaucc ugcaagagcg gggcauuuuu     1380

aguaagccac agccgaaaaa ucgccaguug cugcccccuc ucuccgaauu agcuaaggag     1440

gagucauauu ucgauauacc aaaccccgug cugagaaggg cccuaaguga auuucguaaa     1500

guggucaaug cacuccugga gaaguacggg ggauuccacu acuuucauau cgaacuuaca     1560

cgggacguuu guaaagccaa gagcgcucga augcaguugg agaagauuaa caaaaagaau     1620

aaauccgaaa acgaugcggc aucucaacug cucgaggugc ugggcuuacc uaauacuuau     1680

aacaauagac ugaagugcaa acuuuggaag cagcaggagg aauacuguuu guauagcggu     1740

gagaagauca ccaucgacca ccucaaagau cagagggccc ugcaaauuga ccaugcuuuc     1800

ccacuaucac gcagucugga ugacucccag agcaacaagg uacucugccu gacaucuuca     1860

aaucaggaaa aaucuaacaa gacccccuac gaguggcuug ggagcgacga gaaaaagugg     1920

gauauguaug ugggccgggu cuacuccucu aacuuuucgc cgagcaagaa aagaaaguug     1980

acgcagaaaa auuucaagga aaggaacgag gaagacuuuc uggcccgcaa uuuaguggau     2040

acuggauaua uaggccgagu uacaaaagag uacaucaagc acucccucuc auuccugccu     2100

cuucccgacg ggaaaaagga acacauucgg aucauuagug gaucuaugac cagcacuaug     2160

cguuccuuuu ggggugugca agagaagaac agagaucauc aucugcacca cgcacaggac     2220

gccaucauaa ucgcuuguau ugagccauca augauccaga aauacacaac cuauuugaag     2280

gauaaagaaa cgcauaggcu caagagccac caaaaagccc agauucugcg cgagggcgac     2340

cacaagcuau cucugcggug gccuaugucc aauuucaagg auaaaaucca ggaaaguaua     2400

cagaacauua uccccagcca ucacgucucu cauaagguga ccggggagcu ccaccaagaa     2460

acaguaagaa cuaaagaguu uuacuaucag gcauucggag gcgaggaagg uguuaagaaa     2520

gcgcugaagu ucgggaagau uagggagauc aaucagggca uaguggacaa cggagcuaug     2580

guccgagugg auaucuuuaa auccaaggac aaaggcaagu ucuacgccgu cccaauuuau     2640

accuacgauu uugcaaucgg gaaacuuccc aauaaggcca uugugcaggg aaagaaaaac     2700

gguaucauaa aggacugguu ggaaauggac gagaauuaug aguucugcuu uucacuguuc     2760

aaaaacgauu gcauuaagau ccaaacaaaa gaaaugcagg aggcuguuuu agccaucuac     2820

aagaguacua auagcgcaaa ggcuaccauu gaacucgagc accugucgaa auacgcccuu     2880

aagaacgaag acgaggaaaa gauguuuaca gauaccgaca aagagaaaaa uaagacuaug     2940

acgcgcgagu ccuguggcau ccaggggcug aaaguguucc agaagguaaa gcuaucuguc     3000

cucggagaag ugcuggagca uaaaccucgg aacagacaaa acauugcguu gaagacaacc     3060

ccaaaacacg uuuga                                                      3075


<210>  2600
<211>  3078
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2600
augggcgggu acaugcccca gcugaugaga uauaggcucg cccuugacau cggaagcacc       60

uccauugguu gggcuaugau acggcugucu augcaaaacg agccuaucgc aauuauccgc      120

augggcgugc gaauuuucuc agaugggcgu aauccaaagg acggaaguag cuuggccguc      180

acaagaaggg uugcgcggca gaugcgcaga aggcgggauc gcuuacugaa acgaaagacu      240

agacuacuca acgcucuggu gcagaaaaag uuuuucccgg aagacgccga ugcaaggaaa      300

gcccuuguag ugcugaaucc cuacgaguug cggaaggaag gccucuaucg ucagcugucc      360

gguccugagu uugggcgcgc ucuguuccac aucucucaaa gaaggggauu ucucucgaac      420

cggaagacgg acaaaaagga uaaugacuca agcgucuuaa agaaggcaau aguggaucug      480

cgacagcagc uugaaguuga gaacugccag accaucggcg agugguuguu caaacaacag      540

cagaguggca aguccgugcg cgccagacug agggggaaga cacagaaaga caaggcuuac      600

gauuuuuaua uugaccgggc caugaucgcg caugaguucg auacucuaug ggucucucaa      660

gcaaaauacg acccacuccu guuuaaugaa gacgcccgcg cuauucuuag agauauccug      720

uuguaucaga ggccccucaa gccugugaaa ccaggacguu guacccugau acccgacgaa      780

agccgggccc cuuuagcacu gccacucaca cagcaagcuc gaauuuacca ggagcuuaac      840

caccugagaa ucaccacucc ggccuugggu gcgcaggccc uaacaguagc acagaggaau      900

accauugcug cccugcucga gaaguccgau guuacguucg ugaaaaucgc uaaggcccug      960

ggccuuacuu caaccacaga auuuaaccug caagacauca agcgcgaucg guugaaaggg     1020

aaugcaacug ccauuauacu cgcuagaaac caguacuucg gaaggcgcug gcaugacuuu     1080

aaugcggauc ugcagaacuu aaucgucgac aagcugguga augagggcag ugaaaccgca     1140

cuaauugagu ggcucaaagc caacacagau guggacgaaa gcgcugccga gcgagucucu     1200

gguguuaccc ugcccgaggg guauggaucc cuuucagugg cagccuugcg gcgucugcuc     1260

ccugcucuga aggaagaugu aguggucuac agcgaggccg ugaaaagagc aggcuucgac     1320

agucacucug cgcuuucccu gaucgaacag acgggcgagu uguaucaaga acucccauac     1380

uauggggagg uucugcagag gcauguggga uucgguagcg gcgaccccaa cgauucgccu     1440

gagcgccggu uugggaagau agcuaaucca acuguccaca uuggauuaaa cgaacugaga     1500

aagaucauca augcccuaau uaaaagguac ggcaaccccg cagagguggu aguugaacuc     1560

gcccgagacc ugaaacuuuc ucgcgagaaa cggaaggaaa uccaggagga gcagaaacaa     1620

agacaggaua ggaaugaaaa gcguauuaag gacgcuugcg agauauuggg uaucgauccg     1680

cuggugauug accgcgggaa acggagagaa aucucccaga agaugcagcu cugggaugag     1740

cugaacccua aagacgccgc aaauaggcga ugucccuaua caggcgauca aaucucaauu     1800

gagaaguuac ugagugcuga agucgagaua gaacacaucc uuccauucag caaaaccuug     1860

gacgauuccc ucaacaauaa gacuguggcc augcggcgcg cgaacagaga caagggaaau     1920

aggacaccuu acgaggccuu uggcgaaucu cugaaccccg auuacgacua ugacgcuauu     1980

cuacagcggg caagccugau gccacgagag aaaagaaagc gcuucgccuc agauggguac     2040

cagcaauggc ucagggacga uaaagacuuu cugagucgug cucuuaauga uaccgccuau     2100

uugucccgga ucgcaaagga guaccugacg uuaauuuguc ccccuaacag cgucagagcg     2160

aucccaggaa ggaugaccgc ccagcuccgc gcuauguucg gucugaaucg acuucugucu     2220

gaaggcggga ucaaaaacag agaggaccau cggcaccaug ccauagaugc agcugugauu     2280

ggaguuacag accagggcuu gcuccagaag uuuucaaggg ccagugcacg cacucgugaa     2340

cugcaacuaa gcagacuggu ggaugacaug ccgcuccccu gggauaccua ucgggagcac     2400

guaacaaggg cccuggaaca ccuugucgug ucccauaaac cugaccacgg uuaccagggg     2460

aagaugcaug aggauacugc uuggggcuug cgcgacaacg gacagguuac ccgaagagug     2520

cggucugagg acggcgggcc aaggcagcgc cuguuagaaa auaagaacgu cgugaaaauc     2580

aauucgacaa gagauuucag caggcacgga auugacgagc aaggucaggu aaacgcguau     2640

aagggcuacg uugggggauc caauuacugc aucgaaauau ggauugauga gaaaggcaag     2700

uggaacucag acguggucag uaccuuucag gccuaucaga ucgugcggga acucggugag     2760

aaagcagcuu ggcaaaagcu gcguaauccc cuucugucuc uaagcgccaa gccucucgug     2820

augcgccuga uuaucgauga cgucguuaaa augcagauag ccgggcagaa caagaucaug     2880

cgagugguga aaauugcuca aaauggccag aucuucuuug cagagcauaa cgaagccaau     2940

guagaugcga gagacaagga uaaaaacaau ccauucaagu acauuuccaa gacugcugga     3000

ucauugcaga aagccggcgg gauccacaug gcaauaagcc ccauuggaga cgccaggccg     3060

guccgguaug gccgcuga                                                   3078


<210>  2601
<211>  3084
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2601
augaaguaca cccugggccu cgacgugggg acaaacagca ucggaugggc cguccuugag       60

cugaauacua acggugaacc ccagagaauu gagaaauugg gcuccaggau auucacggau      120

gggcggcacc cuaaggacgg aaccacauua gcuucuaaac gccgacaaaa gcgucaugaa      180

agaaggcggc gcgauagacu gcaccagagg aaaaagcuag uucucaauca gcugguguca      240

augggccuuu uuccacagag ugagcaagaa cagcaggacc uuaagagcuu ggauguacuc      300

acucugcggg caaacggugu guccaaacag cugucugccu augagcucgg gcgcgucuuc      360

uacuauuuaa aucugaagcg aggauuuaaa ucgaacagaa agggcggcuc agaggaagac      420

aaagcgaaua agaucagcga gaggauuaag aaacuucaag aacaguugaa ggagaacgau      480

uucaaaaccg uggggcagua ccugcaugac cgguauauca agggacuaag uacaaaagcu      540

accauugaga augauuuuca ccuccugcgu cagcuuaucg aagacgaguu cgauuuuaua      600

guugaaacuc aauccaaguu cuuuccggag aucucugacg aacaguggaa cacacugcgc      660

aauagaaugu uccaucagag gcccuugaaa ccuguggaga ccgguacgug cagccucuuu      720

gaggauaaga agcggacuua caaauauaug ccauccuucg aagaguaccg auuucugacc      780

gaauuauuca accugucaua ccaagacgag aauuuccaca gucacagccu cucuuccgaa      840

cagauuuuua aggucuucca ugagcuuaaa aacacacgcg aggccacuua uaccaagauc      900

aaaaagcugu ugaagcuacc cucagaccug agcuuuagua uugaaagauc uaaauccaag      960

gauaaaauca agauaagcac aaccaaucac uucuuuucga aaucucuccu guucgcagag     1020

gaaugggccu cccuugaccu gaaguugaag gaugaggugg cugaaauuua cuuuucagac     1080

augaguguag aggagaucaa aaagggccuc cugcaguuaa aaauugauag cgaaaucauc     1140

gagaaccugc uagaagagaa gguuccuaac gugcaggagg caacguguuc cuauucuaaa     1200

gaagcccucc aaaagauugu cagccugaua cuugaggaag agaggcaucc aacuuugauc     1260

guggacgauc uguucaagaa ugaaaaagag gagacaucac ucauugaccg gcuggauuac     1320

uauggggcua guauccccga cuccaugcag ccuauaccac agcacauuaa ggauaacaau     1380

cuuacccuga acgaagacga gaaacgcuac ggaaagaucg ccaauccgac ugugcacgcg     1440

gcauugaacc agaucagaca agucguuaau gaccucauug aacaguaugg caaacccaca     1500

aacauccaua uugaguuugc cagggaucug aagaaaagca aggaggagcg ugacuucgau     1560

aaucggaaga aaaagaaaaa cgaggaauua aaugaacagg ugcgagacuu uauagaaaag     1620

cacaaccaga aaccuucugc uuucaauuuu gagagaguaa agcugugguu cgaacuagau     1680

gccaugaaca aucaagugug cgcauacuca gguaagacca ucagugcuag gaugguccuc     1740

agcgacgagg ugcagguuga ucauauucug ccauucuccc gcacgcuuga cgauucuuug     1800

gccaacaaag ugcugguccu cgcgucggag aacgugaaga aacggaauag aacccccuac     1860

gaagccuuug ggcaggacac agagaagugg gcaagcaucc aggaacuggc uaaaagguua     1920

ccuuaucaca agcaauggcg cuucgccgag gaugcuauca agaaauuuga ggaaaaggac     1980

caguuccugc agcgacaucu uaacgauacu cgguacauuu ccaaaauagc caagaaauau     2040

uugggaucaa ucaccgaccc auacaagauu guagcaaguc guggccaaau gacaucuauc     2100

auuagaggca agcucgggcu gaaucaguuu auccagaaac ccgacggaac uaagaacagg     2160

gaggaucacc gccaccaugc cauagacgcu cuaaccguug cgcugacaag ccgguccuau     2220

cucaaaagaa ucucagaugc aagcgccagg aaucaggacc cgaaccgcau uccuaucccc     2280

gaaccaugga aggguuucau uaaagaugcu gacgugaagu uuaaugagau cauagucucu     2340

cacaaagugg aucauggcaa gaacgggccu uucauggaag agaccuguuu uggacugauu     2400

aagaauccca acaaauacga ggaagagaau gacuucaagc ugaucacuac gaaauccuug     2460

gauaguguca aggacccaag cacaauucga ucucaauccc ugcagaacuu agccgaaaaa     2520

ucaggcguga ucagucucgc aaagagcggu guuaagaaac ugcgggugua ugauguaacc     2580

aaggagaauc ccgccgacau agggcuuauc gaaucgggcc uggcuaaaau uacucacgga     2640

aaggauaaac agcacgucaa gcauuaccag aagggcgaca ucaacuacuu ggccauuugg     2700

ugccucccuc aaaauaucac ccugaaaucc gacaaccaca agaaaagaaa gacagauuau     2760

auauuuaccg caauuaaaac uuucgaccua aacucuaaga auccaaacga ucugaggccg     2820

caucccgcgg cuaagcucgu gacaaaaguu uacaaggggg acacgguggc ccuggagguc     2880

aauaaccagc uuaauuauua caucgugaaa agcauccgug cagccaacaa gaaauuguau     2940

uuucugaaga uuaaucaguc aagugaguua gcuaacgaaa cccaguucau cccugccuac     3000

acuaagcucg uagauuauaa auuccgcaag guguccguuu cuccaacagg agucauuacc     3060

gacaaugguc cccugcuuaa auga                                            3084


<210>  2602
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2602
augagaagcc ugagguaccg gcucgcccuu gaccugggcu ccaccucuuu ggggugggcu       60

uuauuccgcc uggaugcaug caaccgaccc acagccguga ucaaggcggg aguccguauu      120

uucucagacg guagaaaucc uaaagauggc aguagccuag cuguuacuag gcgggccgca      180

cgcgccauga gaaggcggcg cgaccgacuc cugaagagaa aaacgaggau gcaggcuaaa      240

cuuguggagc acggguucuu uccagcagau gccggaaaac ggaaggcucu ggaacaauug      300

aacccguaug cccuccgugc gaagggccug caggaggcac ugcuccccgg ugaauuugcc      360

cgcgcuuuau uucauauaaa ucagagaagg ggguucaaau ccaaccggaa gaccgacaaa      420

aaggauaaug acucuggagu acugaaaaag gccaucggcc agcuucgaca acagauggca      480

gagcagggcu cgcgcacagu gggggaauac uuguggacua gacugcagca aggacagggu      540

gucagggcuc gguaucgcga gaagccuuac accacagagg aaggcaaaaa gagaauugau      600

aaaucauaug accuauacau cgauagggcc augauugagc aggaauuuga cgcgcucugg      660

gccgcacagg cugccuucaa cccaacccug uuucacgagg cugcccgugc agaucuuaag      720

gacacucugu ugcaucaacg gccccuccga ccugugaaac cagggagaug uacacuguua      780

cccgaggaag agagggcccc ucuggcucuc ccaagcaccc agcgcuuccg gauccaccag      840

gaaguuaauc aucuuagacu guuggacgag aaccuaaggg aaguggcgcu gacgcucgca      900

caacgcgaug ccgucgugac ugcucuggag accaaggcca aacuuaguuu ugagcagaua      960

cgaaagcugu ugaagcucuc cggaucugua caguucaauc uggaagacgc aaaacggaca     1020

gaguuaaagg gcaacgccac uagcgcugcc cuggcacgua aagaacuauu uggugcggcu     1080

ugguccgggu ucgaugaggc ccuccaggac gaaaucguuu ggcaacuggu gaccgaggag     1140

ggagaaggcg cacuuauugc cugguugcag acacacaccg gcgucgauga ggcuagagcc     1200

caggcaaucg uggacguguc acugccggaa ggguauggaa aucucaguag gaaggcucug     1260

gcccgcauug uccccgcgcu ucgggccgcu guuaucacgu acgauaaagc agugcaggcc     1320

gcugguuucg accaccauag ccaacugggc uuugaguacg augccucuga aguagaggac     1380

uuggugcacc cugagacugg ggaaauaaga uccgucuuca agcagcuccc auauuacgga     1440

aaggcacugc agaggcaugu ggcguuuggc ucagguaaac ccgaggaucc ugacgaaaag     1500

cgauauggga aaauugccaa cccaacaguu cacaucggcu uaaaucaggu gcgcaugguc     1560

gugaacgcuc ugauucggag auacggaagg cccaccgagg uaguuaucga gcuagcccgu     1620

gaccucaagc aaagccgcga acagaaagug gaggcacagc ggagacaggc ugauaaucaa     1680

aggcgaaacg cccggauccg cagaaguauu gcagaagucc ugggcauagg ggaggaaagg     1740

gugcggggau cugacaucca gaaguggauu ugcugggagg agcuuuccuu cgaugccgcu     1800

gaccgaagau guccguauag cgguguccag aucucggcgg ccauguugcu gucugaugaa     1860

guggagguug aacacauacu cccuuuuucc aagacucugg acgauucauu aaacaaucgc     1920

acaguggcaa ugaggcaagc uaaccguauu aaacggaaua gaacccccug ggacgccagg     1980

gccgaguucg aagcucaggg cuggaguuac gaggauaucc ugcagcgcgc agagcgaaug     2040

ccacuuagaa agcgguauag guuugccccu gacggguacg aacgcugguu gggagaugac     2100

aaagauuucc ucgcgcgugc ucugaacgac acgagauacc uaagccgggu agccgcagag     2160

uaucugaggc ucgucugccc cggcacccgc gugaucccag gucagcugac agcccuuuug     2220

cgagggaagu uuggccugaa ugacguuuua ggacucgaug gcgaaaagaa cagaaaugac     2280

caucggcacc augcugugga ugcauguguc auugggguga cugaccaagg acugaugcag     2340

agguucgcca ccgcguccgc ucaggcccgc ggugauggcc uuacaagacu gguagacggg     2400

augcccaugc cuuggccaac uuacagggau cacguggagc gggcaguucg ucauaucugg     2460

gucucucacc gcccggacca cggauuugaa ggcgccauga uggaggagac cagcuauggu     2520

auucgaaagg augggucaau aaagcagaga aggaaagcug acggcagugc cggacgggaa     2580

aucuccaacu ugauucgcau ccaugaggca acacaacccc ucagacacgg cgugagcgcu     2640

gaugggcagc cucuggccua caagggauau gugggcgguu cuaauuacug caucgaaauu     2700

accgucaacg acaaagggaa gugggaggga gagguuauau caacuuucag ggcguauggc     2760

guggugcggg ccgguggcau ggggcgacua agaaauccac augaaggaca gaacggccgc     2820

aaacugauca ugaggcucgu aauuggggau aguguccggc uggaggugga cggagcagaa     2880

cguacgauga gaaucguuaa gauuagcggc uccaaugguc agaucuuuau ggcucccaua     2940

cacgaggcca acguggacgc uaggaauaca gauaaacaag acgcauucac cuacacuucu     3000

aaguacgccg ggucgcuuca gaaggccaaa acccgccggg ucacaaucag cccuauugga     3060

gaagugcgag auccaggcuu caaggguuga                                      3090


<210>  2603
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2603
augaaguaca gacuggcccu cgaccuuggc agcaccuccc uggggugggc uaucgugcag       60

uugaacgcaa aagaugagcc cugcgccauu gucagggcgg gaguucggau auucccugac      120

ggucgcaauc caaaggaugg aucuucauua gcugugacac gacguaacgc cagagcaaug      180

aggcggcgca gagacaggcu gcuaaaacgg aaggcccgca ugcaacgacu ccugaucgaa      240

cacgaucuuu uuccggcuga cgaggcagcc agaaaagcuc uggaaacuuu gaaucccuau      300

gagcucaggg ccaaggggcu ggaugcggca cugacgccug ccgaauuugc ucgggcccuc      360

uuucauuuaa accagcgucg cggauucaag aguaauagaa ggaccgaccg gaaagauaac      420

gacagcggcg cacugaaggc ugccauuggu gggcuucgag cgcaguugga uacagccgga      480

gcagacggcc gcgcuagaac uguaggcgag cugcuaugga ggcggauguc cgauccaaau      540

gugcucaccg uccgcgccag auacagggag cagcguauca caaaagacga cgggaagacc      600

cggguggaua aaucuuauga ccuguacguu gaucgagcua ugguggaagc cgaguuugac      660

gcacuuuggg ccaagcaagc ugaacugucg cccacucagu ucggagaggc ggcaagagcc      720

acauugaagg auugucuccu gcaccagagg aacuuacgcc cuguccggcc agguagaugc      780

acccuggagc ccgacgaacc uagggcucca cucgcccuuc cgucacagca acgcuuucgg      840

auucugcagg aggugaauca uuugcgacua cugggcgggc gucuccugga agagagccuu      900

aguguagaac agagagauaa acugguugca gccuuggagg acggauccaa gucuuucaaa      960

acggcuaucc ccaagcuccu gggcuuaccu gguacugugc aguuuaaccu ggccgaugca     1020

aaaaggaccg agcuaaaggg gaaugcgaca agcaugcucc ugggcaaaaa ggacuucuuu     1080

ggcccagcuu ggcacggcuu cuccgaagcc cgccaagaug agauagucau gcggauccag     1140

gaagaggaau cagaggcaag acuuguggac ugguuggcca ccaguacugg gguggacgag     1200

gcucaugccc ugaagauugc aaacgcugcc cuccccgaag gauaugguag ccugucugcg     1260

aaagcccuug cuaagauccu gccugcaaug agggccgagg ucauuacaua cgauaaagcu     1320

guuuuggccg caggcuauga ccaccacucc cauaucucac acgcggccag caccggggaa     1380

auacucccag agcugcccua cuacggagaa uauuuacagc gacauguggg cuuugguacg     1440

ggggagcuga augauacucc ggagaagcgc uucggccgga uugcuaaccc uacaguacac     1500

aucggacuaa aucaagugag agucgugguu aacgcccuca uuaaaaggua cggccacccc     1560

accgaaguga ucgucgaggu ggcacgugac cugaagcaga gucaggauca gcgccgggaa     1620

acucaagagc agcaggcuaa gaaccagaaa agaaaugaga ggcuucgaaa ggacgccgca     1680

gccaucuugg ggauugcuga ugaacgggua cgccuggcgg acauacaaaa acucauccug     1740

ugggaggaau uaucuuucga uauugccgac agaagguguc cauauuccgg aguucagcug     1800

agcgcagcua uggugcuuuc ggagcagguc gaaaucgagc auauauugcc uuuuucuaug     1860

acacucgaug acucccugaa caauaagacc guggccaugc ggcuagucaa ccgacugaaa     1920

gguaauagaa cgcccuggga ugcccgcgag gcuuucgcag gccaggggug ggacuacgac     1980

gggauucuca ugagggccga acguaugcca cgggcgaaga gacaaagguu ugcucccgau     2040

ggccuggaga aguggcuuaa agaagacaag gauuucuugg cccgcgcacu gaacgacacc     2100

cgacacuuau caagaguggc ccgggaguau cucagucugg uuugcccugc uagcacaagg     2160

gugaucccag gucagaugac ugcaaugcuu cgcgccaaau uugggcugaa ugauaucuug     2220

ggccucaacg gagaaaagaa ucguaacgac cauagacacc augcgguaga ugcuuguguc     2280

guggccguua ccgaccaggg ccugcuacag cgguucgcac aagccuccgc ugaugccagg     2340

gggaaaggac uggaccgccu cguggagaca augccggauc ccugggacuc uuaccgagag     2400

cacguccaga gagcagugaa uagcauuugg guaucacaca agccugauca ugguuuugaa     2460

ggcgcuaugc acgaggacac uaccuaucgg ccaccccauc uggacccuaa gaacaugugg     2520

aggacacgcg ggaucggagg cgguaaacca aaugaaaagg aggccgauag uaaagcgaug     2580

gugggguuca ccggccccga acaccuucag agacaccaua gggacaaggc cggacaacag     2640

uugccguaca aaggcuacgu ugcauccccu aaguuuugca uggauauugu ccggaacgac     2700

aaagggcguu gggagggaga gguggugagc acuuucgagg cuuaucgccu ggucucugaa     2760

uuuggccgag augccgcuuu agcacacaua cagucaaaga guagaagcau cuccgguaag     2820

ccacucguua ugaggcugca gggaggagac auggugcggu ucgaugacga ugaaggccgc     2880

cccgugguac uuagagucgu gaauaugacg gguaacggcc aaauuuucuu cgccccucau     2940

aacgaggcca auggggacgc uaggaaccgg gauaaacagg accaguucgu uuaccugucu     3000

aaguaugcgg gaucgcuaca gaaagcacga ggcagacgcg ugacagucag ccccgccggg     3060

gagcucaggg acccaggauu uaaggaauga                                      3090


<210>  2604
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2604
augagaagcc ugagguaccg gcucgcccuu gaccugggcu ccaccucuuu ggggugggcu       60

uuauuccgcc uggaugcaug caaccgaccc acagccguga ucaaggcggg aguccguauu      120

uucucagacg guagaaaucc uaaagauggc aguagccuag cuguuacuag gcgggccgca      180

cgcgccauga gaaggcggcg cgaccgacuc cugaagagaa aaacgaggau gcaggcuaaa      240

cuuguggagc acggguucuu uccagcagau gccggaaaac ggaaggcucu ggaacaauug      300

aacccguaug cccuccgugc gaagggccug caggaggcac ugcuccccgg ugaauuugcc      360

cgcgcuuuau uucauauaaa ucagagaagg ggguucaaau ccaaccggaa gaccgacaaa      420

aaggauaaug acucuggagc ccugaaaaag gcaaucggcc agcuucgaca acagauggcu      480

gagcagggcu cgcgcacagu aggggaauac uuguggacua gacugcagca aggacagggu      540

gugagggccc gguaucgcga gaagccuuac accacagagg aaggcaaaaa gagaauugau      600

aaaucauaug accuauacau cgauagggcg augauugagc aggaauuuga cgcccucugg      660

gcagcucagg ccgcuuucaa cccaacccug uuucacgagg ccgcacgugc cgaucuuaag      720

gacacucugu ugcaucaacg gccccuccga ccugucaaac cagggagaug uacacuguua      780

cccgaggaag agagggcucc ucuggcgcuc ccaagcaccc agcgcuuccg gauccaccag      840

gaagugaauc aucuuagacu guuggacgag aaccuaaggg aaguugcacu gacgcucgcc      900

caacgcgaug cuguggucac ugcccuggag accaaggcaa aacuuaguuu ugagcagaua      960

cgaaagcugu ugaagcucuc cggaucugug caguucaauc uggaagacgc caaacggaca     1020

gaguuaaagg gcaacgcuac uagcgccgca cuggcgcgua aagaacuauu uggugcugcc     1080

ugguccgggu ucgaugaggc acuccaggac gaaaucguau ggcaacuggu uaccgaggag     1140

ggagaaggcg cccuuauugc uugguugcag acacacaccg gcguggauga ggccagagca     1200

caggcuaucg ucgacguguc acugccggaa ggguauggaa aucucaguag gaaggcccug     1260

gcgcgcauug ugcccgcccu ucgggcugca gucaucacgu acgauaaagc cguucaggcu     1320

gccgguuucg accaccauag ccaacugggc uuugaguacg augcaucuga aguggaggac     1380

uugguacacc cugagacugg ggaaauaaga uccguguuca agcagcuccc auauuacgga     1440

aaggcgcugc agaggcaugu cgccuuuggc ucagguaaac ccgaggaucc ugacgaaaag     1500

cgauauggga aaauugcuaa cccaacagug cacaucggcu uaaaucaggu ucgcauggug     1560

gucaacgccc ugauucggag auacggaagg cccaccgagg ugguaaucga gcuagcacgu     1620

gaccucaagc aaagccgcga acagaaaguu gaggcucagc ggagacaggc cgauaaucaa     1680

aggcgaaacg cacggauccg cagaaguauu gccgaagugc ugggcauagg ggaggaaagg     1740

guccggggau cugacaucca gaaguggauu ugcugggagg agcuuuccuu cgaugcugcg     1800

gaccgaagau guccguauag cggugugcag aucucggccg caauguugcu gucugaugaa     1860

gucgaggugg aacacauacu cccuuuuucc aagacucugg acgauucauu aaacaaucgc     1920

acaguugcua ugaggcaagc caaccguauu aaacggaaua gaacccccug ggacgccagg     1980

gcugaguucg aagcacaggg cuggaguuac gaggauaucc ugcagcgcgc cgagcgaaug     2040

ccacuuagaa agcgguauag guuugcgccu gacggguacg aacgcugguu gggagaugac     2100

aaagauuucc ucgcucgugc ccugaacgac acgagauacc uaagccgggu ggcagccgag     2160

uaucugaggc ucguaugccc cggcacccgc gucaucccag gucagcugac agcucuuuug     2220

cgagggaagu uuggccugaa ugacguguua ggacucgaug gcgaaaagaa cagaaaugac     2280

caucggcacc augcaguuga ugccugugug auugggguca cugaccaagg acugaugcag     2340

agguucgcga ccgcuuccgc ccaggcacgc ggugauggcc uuacaagacu gguggacggg     2400

augcccaugc cuuggccaac uuacagggau cacguagagc gggccgugcg ucauaucugg     2460

guuucucacc gcccggacca cggauuugaa ggcgcuauga uggaggagac cagcuauggu     2520

auucgaaagg augggucaau aaagcagaga aggaaagccg acggcagugc aggacgggaa     2580

aucuccaacu ugauucgcau ccaugaggcu acacaacccc ucagacacgg cgucagcgcc     2640

gaugggcagc cucuggcgua caagggauau gugggcgguu cuaauuacug caucgaaauu     2700

accgugaacg acaaagggaa gugggaggga gaggucauau caacuuucag ggccuauggc     2760

aucguucggg cugguggcau ggggcgacua agaaauccac augaaggaca gaacggccgc     2820

aaacugauua ugaggcucgu gaucggggau agugugcggc uggagguaga cggagcagaa     2880

cguacgauga gaauugucaa gaucagcggc uccaaugguc agauauuuau ggcccccauc     2940

cacgaggcua acguggacgc aaggaauaca gauaaacaag acgccuucac cuacacuucu     3000

aaguacgccg ggucgcuuca gaaggcuaaa acccgccggg uuacaauuag cccuaucgga     3060

gaagugcgag auccaggcuu caaggguuga                                      3090


<210>  2605
<211>  3096
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2605
augcccgcca ucuacagacu ggcucucgac cuuggcagca ccucccuggg gugggcagug       60

uugagguuag agccugccgc gcuggaacca auugcuauaa ucaaggccgg aguccggauu      120

uucucugaug gucgcaaccc gaaagacggc gcaucacuag ccguuacacg acgugcugca      180

agagccauga ggcggcgcag agauaggcuc cugaagcgga aagcucgcau gcaggacaaa      240

cuugugaaac acggguuuuu ccccagugau auggacgagc gaaaggcccu ggaaagauug      300

gauccuuaug cgcucagggc aaagggacug cgggaggccc ugacuccagg cgaauuugcu      360

cgugcccucu uccauaucaa ucaacgcaga gguuuuaaaa gcaacaggaa gacggacaaa      420

aaggauaaug acuccggggc auuaaagaag gcuauuucuc accugagaca gcagauggag      480

gccggaggcc aucgaaccgu aggcgaucac cuuuggucgc gcuugcagca agggcuggga      540

gugagagcga gguaccggga gacacccuuc acuaacgacg aagguaagaa acgcaucgau      600

aagucauaug accuauacau agacagagcc augaucgagc aggaauuuga ugcacucugg      660

gcugcccagg cugccuucaa uccugcacug uuucaugagg ucgccagggc ugaccuuaaa      720

gauacccugu ugcaccagcg ucggcuccga ccagugaagc ccggcagaug cacacuguua      780

ccugaggaag agagggcgcc acuggcacuc ccgagcaccc aacgcuuccg gauuuaucag      840

gaaguuaacc accuuagacu guugggggag gaccuaaggg aaguggcccu gacucucgcu      900

cagcgcgaug ccgucgcaaa ugcccuggag aguaaagcuc gacuuacauu ugagcagauc      960

cggaagcugu ugaaacucuc cggaucugug caauucaacc uggaagacgc caagcguacc     1020

gaguuaaagg gcaaugcaac gagcgcggcu cuggccagaa aagaacuauu uggugcagcc     1080

uggggagggu ucggcgaggc ucuccaggau gaaauuguau ggcagcuggu uacugaggag     1140

ggcgaagggg cccuuaucgc augguugcaa gcucauaccg gaguggacga ggccagggcg     1200

caggccauag cugaugcauc ccugcccgaa gguuacggcu cacucagucg caaggcccug     1260

gcucggauug ucccugcccu uagagcaggg guggugacau augacaaagc gguccaggcc     1320

gcuggauucg aucaccauag ccagcugggc uuugaguacg acgccgguga aguugaggac     1380

ugggugcacc cagauacugg ggagaucagg cccguauuca agcaauugcc uuacuauggc     1440

aaaccacucc agcgacacgu ggcauuugga ucuggcaagc ccgaagaccc ggaugagcgc     1500

cgguacggga agauugcuaa cccuaccguc cauaucggac ugaaucaggu gagaacaguu     1560

gugaacgccu uaaucaggcg uuauggucgc cccaccgaag ucgugauuga gcuggcacgg     1620

gaccuaaaac aguccagaga gcaaaaggua gaagcccaga ggaaacaggc ugagaaucag     1680

cgacggaacg cgcgcauaag aaagucaauc gccgaaguuc ucggcauugg ggaggaacaa     1740

gugaggggag cagauaucca gaaauggaua uguugggagg agcugagcuu cgacgucgcu     1800

gaacggcgau gcccauacag uggcgugcag auuucugcca gaaugcuuuu guccgaugag     1860

gucgaagugg agcacauccu gccuuuuagc aagacgcucg acgauucgcu gaauaaccgc     1920

acuguugcca ugaggcaggc uaaccguauc aagcggaaua gaacacccug gaccgcaagg     1980

gacgauuucg aagcccaagg uuggucuuau gaggggauuu uacagcgcgc ggagcgaaug     2040

ccacugagaa aacgguacag guuugcuccc gacggcuaug aacgcuggcu uggagaugac     2100

aaggacuucu uggcccgugc acucaacgau acuagauacc ugucccgggu ggccgcugag     2160

uaccuaaggc ugguaugucc uggcucaggg acacgcguca ucccaggaca gaugaccgca     2220

cgacucagag guaaauuugg ccugaauggg gugcuuggac cggacggcga aaagaaccgg     2280

aaugaucaua ggcaccaugc cguugacgcg ugcgugauug gugucacgga ucaaggguug     2340

augcagcgcu ucgcuaacgc cagugcacag gccagagagg acggccugac cagguuagug     2400

gaugacaugc cccggccuug gccaacauau cgugcucacg uagaacgcgc cgugcgacac     2460

auauggguua gccauagacc cgaucacgga uuugagggcg caaugaugga ggaaacuucc     2520

uacgggaucg gaaaagacgg cucuauuaag cagaggcgga aggcugaugg uagcccuggg     2580

cgcgagauca ccaaucucau ccggauucau gaagccacac aaccacagag gcacggagcg     2640

ggccccgacg gucagccgcu gccuuauaaa ggcuacgucg ggggaucaaa cuauuguaua     2700

gagaucacuc ggaaugauaa gggcaaaugg gagggggaag ugauuaguac cuuccgagcc     2760

uacggaaucg ugagaacauc cggccuuggu cagcugcgcc acccaagggu cgggcaaaac     2820

ggacgggagu ugguuaugcg ucucgugauu ggcgacagcg ugagacugga aaucgacggu     2880

auagagagga ccaugcgcau cguaaagauu ucuggcucaa augggcagau cuuuauggca     2940

cccauucaug aagcuaacgu cgaugcccgg aauagugaca aacaggaugc uuucgcauau     3000

acuagcaagu acgccggauc ccuacagaaa acgaaggccc gauucgugac aaucucuccu     3060

auaggcgagg uuagagaccu gggguuuaag ggauga                               3096


<210>  2606
<211>  3099
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2606
augaagaaaa gaauccuggg ccucgacauu gggauagcca gcgugggaug ggcucuuguc       60

gaguucgaug acgaauuuca ggauaacaag aaaaagggua ccaucauuaa auccggcguu      120

aggaucuuca cacgggcaga gacucccaag gaacgcgagu cucuggccaa gccucgacgu      180

gaagcgagau cacaaaggcg gcgcauuaga aggcgggugg agcgcuugaa uaguauccga      240

gaguuauuug uaaagaacgg gguggucgaa gaguggagcg uguccaagaa aaauccaaac      300

aauauauaca ucacgcugaa ccagauuaag accccguggc agcuaagaau ggaaucucuc      360

gacaggaaac ugacaaauga gcaguucgcu guugugcuua cucaccuggc caagcaucgg      420

ggauauaagu cguugcguaa agaugacaag gcaggcaaag uccucgaggc caucucacaa      480

aacaagaaac gccugaauga uagcgacuac agaacccugg gugaaauguu uugcaagaac      540

gagaguuaua aagaacacaa gaggaauaaa gggggagagu acauuaacuc cguggcucgg      600

gaacucaucg aggaggaagu agagaaaaua uucguuaagc agcgagaaaa aggcaauaac      660

uuugcagaug ugaauacaga gcgcgaauau uuaaagauug ccuucucuca gaaacccgcu      720

ggcagcaucu ccaagauggu cggguuuugu accuucgaga acggagagaa gagagccccu      780

agggacucau acagugcgga acuguuuaaa gugcuuugca cuauuaacaa uacaaccuuc      840

acggauauga agacugguga gauccggagc uuuucuaaag aagagaucgg caagaccuug      900

gaaacaguga aaaacgucaa ggacguuaag uaugcaaaaa uucgcaagga ucuggggcua      960

gaggacuccg ugaaauucuc aggaguagac uacagagaga augccaagag caaaggcgaa     1020

ggugagauau ucgugaguau gaagacuuac caugaaauca agucuaaaau ugagaagcuc     1080

uccaaagaga agugggauaa ccugaaaggg aauaccgaac uucuggacag caucgucaac     1140

auaauugcua auuugaagga ugacgagucg aucaaaaagg aauuucagaa gcucaaaauc     1200

gaugaggaca uuaucgaugu gcuguuaaac uucgaauuua agaaauucau uaaucugucu     1260

uuuaaggccc ucaggaaaau aaucccauuc caauuucagg gacagaagua uauuacagca     1320

uccaccucag cuggcuacag ucuuaagcag gagggcauca aaaacaagcu gguucccccu     1380

gccaaagaga auaucaacgu ggcggucgug gcccgugcau ucgcucaaac gcggaaggua     1440

auuaaugccg uuauacgaaa auaugggcag uuugaccaga ucaacauuga auuggcuacu     1500

gagcuaaaga auagcaaguu cgauagaucc aaaaucgaag agggacaaaa gaaauuucag     1560

ucucagaagg acaaaauuuu caaggaagug caggagcuga gggguggcau gacaccaagc     1620

ucaagucucc ugcuuaagua ccgccugugg cgggagcaag augaaagaug ugucuauucc     1680

gggaggaaaa ucagcuuuga ggacuugcuc gacaccggau uccuggauau agaccacauc     1740

auucccuacu cuaagucaau ggaugacagu uucaacaaca aaguguuaug ccuggccgaa     1800

gagaaucgca acaagaaaaa ugauaucccu uuuaacuauu accagcgaau ugaccgggau     1860

uggaaucuac ucaucagcau aauuacuucc augaagaaca ugaaaaucgc aaagaagucu     1920

cgucugcuua aacaggaauu gucggacauu gagggcuuca ucgagagaaa uauaaacgau     1980

acaagguacg ccacccgcua uaucaaggac uuucuggaaa auaaccucga guucaaagaa     2040

aaugaguuua uuaaggucaa agugcaggcu cggagcggug ggcugacguc cguucuuuca     2100

uacaacuggg gcaugaguaa ggauagagag gaaucuuauu uccaucacgc gaaggacgca     2160

aucauugugg ccuguagcac ccaaggaaug guacaguaca ucuccaaaau aucacagcag     2220

uaugagaaug auaaggacaa aaugaagaaa auuaaggaca agacagaaaa cugcauccca     2280

aaaccguggg auagcuuuag ggcugacguc gaggaaucua ucgagggcau uuucgugucc     2340

uuugcacccc gacacaaggu uacugggagu gcccauaaag agaccaucua cagcaagaaa     2400

caccuggaaa aggagcgcua ugugacaguc aagaaaucuu uggauaagau uaaacucucc     2460

gaccuggaaa auauaccuug uaacgagaac ugcggaauca uucggguguu aaaggaaaga     2520

cuggagaaau ucaagaauga uucacuaaag gccuuugcug acccaguaca uaugcccacu     2580

aaauguccua acaagaaagg uccagugauc aggaguguua agauuaaaga gagcaauaag     2640

accggcaucg aaguccguaa agggcucgcc gagcgcggag aaauggugcg gguggauguc     2700

uucauaaagg acaagaaaua cuaccugguu cccaucuaug ugucggauuu uaagaacaaa     2760

gaccuuccga auaaggcaau ugcggcuuug aaacuggaga acgaauggau caugauugau     2820

aagacauacu ccuucaaguu cucucucuuu aaagacgauc ugaucaagau uaaaaagaaa     2880

aaggaggaga uauucggcua uuuugguggg gugcacagaa gcaccggcac uaucaagauc     2940

aaaucaaggc acaagccuaa agaaauugac gagggaauag gcagucgaug cuuacuggac     3000

uuccggaagu uucaaguaga uguccuuggg aauuacacgg aagugaaaca ugagaagcgc     3060

augcccgccu auaucacaag aaaggacaaa aagcacuga                            3099


<210>  2607
<211>  3105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2607
augaagaucc ugggcuucga cauugggaua gccagcaucg gaugggcuuu uguggagaac       60

ggugaacuca gagauugcgg cgucaggauu uucaccaaag cagagaaucc caagacaggg      120

gacucccuug ccaugccucg gcgcgaagcg cgaucuguuc guagaaggcu ggcucggcgc      180

aaaggaagau uggagacuuu aaagaggcug cuagccaaag aaugggaucu cuguuacgag      240

gacuauaucg cagccgaugg cgagcugcca aaggcuuuua uggguaagaa ccuuacgaau      300

ccguacgugc ugcgguauga agcauugcag cgccuccugg ggaaagagga acuggcccga      360

guaauucucc acaucgcuaa gcauagagga uacggcaaca aaaaugccaa gauaaccaaa      420

ucagaggaga guaagaggga acaaggcaag aucuuaagcg cgcuggcaac aaacgccucc      480

gugauugcuc gguaucguac ugucggggag uacuucuaua aagaauuuug cgaggugauc      540

aagaaucccc agggacuuaa caccaaugaa aacuguacac agccuaaagu ucgcguguug      600

aagccaauua gaaauaaagg uggcgaguac accaacugca uccugcagga ggaccuacaa      660

agggaacucc gguguauauu cgagcaccag aagggguuug gauuccccau uacucaggaa      720

uuucaggaua aaauccugaa gauugccuuc uaccaacgau cucuuaagga cuuuucgcau      780

cuggucggca aaugcacauu cuauccugau gagccacgcg cacccaaguu uucauugagc      840

gcuaucgagu ucaucaccaa agccaagacg auuaaucucc ugguggguau aguaaaagag      900

aguggggagg uuugggacaa ggaacagugg agagagaggu uagauuccgu guucucugcg      960

gucugugaac ggggaaaccu gacuuuugcc cagaugcgca agauccucaa aauugacgag     1020

agaauccagu ucaaggaagu ggauuacacc gcaaagaauc cugagaacaa gaaauuugug     1080

gaguucaaga accuuaagaa auuuugcgaa auacugggcg cuauugucgg cgacagggcc     1140

uuggcugaca gcaucgcccg ugauaugaca cuaaucaagg acgaggaaga acuggcacaa     1200

aaacuccggg gguauggaaa guucucccag gagcagauua cucaacuggg ugcccuuaau     1260

uuuucacacc auaucaaccu gaguuugaaa gcucucagcg aaauucugcc auucaugcga     1320

gagggcauga gauacgauga agcgugucag gaggcaaagu uacaggccaa gcacaaugac     1380

aaaaagucua aauuucugcc gcccuucugc ggguccauau augcugauga acuaaccaac     1440

ccuguugugc acagggccau cgcagaguac cgcaagauuc ucaaugcccu gaucgcuaaa     1500

uauggaaacg uacauaagau ccacauugag cuuacacggg acgugggcaa aaauuuucag     1560

gaaagagaga aguacaagaa agaaauagag ucaaacuaca aggccagggu ccaagcaaug     1620

caggaaugug agaaauuggg ucugacccuc agcgagggga auauccugaa acuucgccug     1680

uucauggaac agaacgagcg augcguguau aguggccgga aaauuacguu ggcgaaucuc     1740

aaggaacagg gagcucugga gaucgaucau auuuuaccau acucucguuc cagcgacgau     1800

ucguauauga acaagguucu gguguuuacu aaugaaaacc aaaauaaagg caacaagaca     1860

cccuacgagg ccuucggggg agacucucag aaauggggug agaucgaauc ccuagcacuc     1920

agaucaggcu auccuaagaa aaaggccaag aggauacugg auaaaggguu cggagaccgc     1980

gaggcuggcu uuaagagucg gaacaucguc gacagcgguu acauugccag acuuaucgca     2040

aauuauacca aagaauacuu gaaguuccug ccacucgauu cccacgagaa cacugcucug     2100

auugccgggg agaaagacuc uaagauccac guggaagcgg uaaagggcau guuaacagcc     2160

accaugaggc auuuuugggg acugggcagc aaaaaucgau acgagcacac gcaucacgcu     2220

auagaugcaa uuaucauugc cuauaucaac gcugccauga uacagcgcuu cucacaguuu     2280

cggcaaaauc aggaaagucu uaaggcaaga uucuacgcga aagaguuggc caaggaagag     2340

uuuaaaaccc agagggcuuu cuuugagccc uucuccgggu uucgugaaca gguucucgcc     2400

aaggugggac aaaucuucgu cagccgcccg ccucggaaaa gagcaagggg ugcucugcau     2460

gaggaaacau uuuauucuau ugacgauaag aagcuaucag agacuuacgg cgggaaagaa     2520

ggagugcagc gagcccugag ucucggcaag auccggcaga uugguaccaa aaucgucgca     2580

aacgggccca uggugcgcgu ugacauauuc aagcacaaaa agagcggcag auucuaugcc     2640

gugccaauuu acacaaugga uuuugcucug ggaauccuuc cuaauaaggc gguagucucc     2700

ggcaaagaca acgauaggau cauuaaggac ugguuggaga uggauucuaa uuaugaguuc     2760

ugcuuuucgc uguucaaaga cgaugugauc gaaguucaaa agaaagacau ggagaagccc     2820

gaauuagccu acuuugugag ccucaacacu uccucagcaa guauugcucu ggucaagcac     2880

gacaauaaau ucgagacccu uacaucuaac caggccaagc uguuuagcaa ugccaaagaa     2940

aacaauguga uagcugaguc cuuggggauc cagaaccuca agguauucaa aaaguacaag     3000

gugucaccac ugggagagau uaaagaagca aaguaugagc cccggcagcc uaucgcccua     3060

aaaaccagcc caaagaaaca ucgaaagcaa aacaaugcgc aguga                     3105


<210>  2608
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2608
augagaaggc ugggccucga ccuugggacc aacagcaucg gauggugccu guuggauuua       60

ggugacgaug gcgagcccgu guccauuuuc cggacagggg cccgcauauu uucugacgga      120

cgagauccua agucacuggg cagucuaaaa gcuacucgua gagaagcaag gcucacgcgg      180

cgcagaaggg accgguucau ccagcgccaa aagaaucuga uuaacgcccu ugucaaauac      240

ggucugaugc cagcggauga gauccagcga caggcuuugg ccuauaagga cccguacccc      300

auuaggaaaa aggcacucga ugaagccauc gacccuuaug agauggggag agcuauauuu      360

cacaucaauc agcggcgugg auucaagagc aaccgcaaau ccgcagauaa ugaagccggc      420

guugugaagc aaucuauugc ugaccuggag augaaacugg gcgaggccgg ggcgagaacc      480

aucggagaau uucucgcaga uaggcaggcc acaaacgaca cuguacgggc ucgacgcuua      540

ucggguacca augcccugua cgaguucuau ccagacagau acaugcuuga acaggaguuu      600

gauacauugu gggcaaagca ggcugccuuc aaccccucac uguauauuga ggcggccagg      660

gaacggcuaa aagagaucgu guuuuuccaa cgcaagcuca agccucagga agucggcaga      720

uguauauuuc ugagcgacga ggauaggauu aguaaagcac uuccauccuu ccagcguuuu      780

cggaucuacc aggaacuguc uaauuuggcu uggauugacc augauggggu ggcccaccga      840

aucaccgcua gccucgcccu gagagaccau uuauucgaug agcuggagca caagaaaaag      900

cucacuuuca aagcaaugag ggccauccuu cgcaagcaag gaguugugga cuaccccguc      960

ggcuuuaacc uggaauccga uaaucgggac cauuugauug guaaccuaac aucaugcaua     1020

augagagaug cuaaaaagau gaucgggagu gcgugggaca ggcuggacga ggaggaacag     1080

gauagcuuca uucucaugcu gcaggacgau caaaagggag acgaugaagu gcgcucuauc     1140

cuuacccagc aguauggccu guccgacgau guagcagagg acuguuugga uguucgacuc     1200

ccugacggcc acgggucacu gagcaaaaag gccauagauc ggauuuuacc agugcugcgu     1260

gaccagggac uaaucuacua ugaugcuguc aaagaggccg gucucggcga agcaaaucug     1320

uacgacccgu augccgcucu uagugacaag uuggauuacu augggaaagc ccuggcagga     1380

cacgugaugg gcgcgucugg uaaguuugag gacuccgaug aaaagagaua cgggacgauc     1440

agcaacccca cugugcauau ugcucucaau caagucaggg ccguugugaa cgagcugauc     1500

cgccuucacg gcaaaccuga cgaaguagug auugagauag gacgggaucu gccaaugggc     1560

gcagacggga agagagaguu ggaaagguuc cagaaagagg gacgagccaa gaacgaacgc     1620

gcucgggaug agcucaaaaa gcugggucau aucgacucga gagagucuag gcagaaguuu     1680

caguuauggg aacaacuggc caaagagccc gucgaucguu gcuguccuuu caccggcaag     1740

augaugucca uuucagaccu auuuagugau aaaguggaaa ucgagcaccu ccugccauuc     1800

agccuuacau uggacgauuc cauggcaaau aagacuguuu gcuuucgcca ggcuaaccgg     1860

gacaaaggga auagagcccc cuucgacgcg uuuggaaacu cuccggccgg cuacgauugg     1920

caggaaaucc uggguaggag ccagaaucuc ccuuaugcua agcgauggcg guuccugccc     1980

gacgcaauga agcgcuuuga ggccgauggg ggcuucuuag agagacaacu gaacgacacc     2040

agguacauuu cacgguauac aaccgaauac auaaguacga ucauuccuaa aaauaagauc     2100

ugggugguca cuggacgacu uacauccuug cucagaggcu ucugggggcu gaacagcauu     2160

cuacgcggac acaauaccga ugacgguacu ccagcuaaaa agucuaggga ugaccaucgu     2220

caccaugcca ucgaugcaau agugguaggc augacaucac gggggcugcu ccagaaaguu     2280

aguaaggcgg ccagaaggag cgaggaccug gaucuuaccc gcuuguuuga aggacgaauc     2340

gaccccuggg acggcuucag agaugaggug aaaaagcaca uugacgcuau cauugucucc     2400

caucggccaa ggaagaaauc ucagggugcc cugcacaacg auacggcaua ugggaucgug     2460

gaacacgcug agaauggcgc cucgaccguc gugcaucgcg uucccauaac uagcuuagga     2520

aagcaguccg acauugagaa agugcgugau ccucucauca agucagcacu gcuuaacgaa     2580

acagccggcc ugagugggaa aucuuuugag aaugcuguac aaaaguggug ugcggacaac     2640

agcauuaagu ccuugagaau cgucgaaacc gugucaauaa ucccaauuac agauaaagag     2700

ggaguugccu acaagggcua uaaaggugac gggaaugcau acauggauau cuaccaggac     2760

ccgacuagcu cuaaguggaa aggagaaauu gugucccggu ucgaugcuaa ccagaagggc     2820

uuuaucccca guuggcaaag ccaguucccu accgccaggc ucauaaugcg ccugcgaauu     2880

aacgaccuac ugaagcucca ggauggugag aucgaggaaa ucuauagagu ccagcggcug     2940

ucuggcucca aaauucuuau ggccccacac acagaggcua auguggacgc aagggaccgc     3000

gauaagaacg acaccuuuaa auugacuuca aagagucccg ggaaacugca aagcgccucg     3060

gcgagaaagg uacauaucuc cccuacggga uuaauuaggg aaggcuga                  3108


<210>  2609
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2609
auggagcagc ugaacuacau ccucggccuu gacauuggga uagccagcgu gggaugggcu       60

gucguugaaa ucgaucacaa ugaggacccc auuagacuga ucgauguggg uguaaggacc      120

uucaacaagg cagaaguggc caaaacaggc gaguccuugu cuuuaauucg gcgccuggcg      180

cgagggucac guagacuggu caggcggaag aaaaugcgcc uccugacugc uaagagacuu      240

cugaaaagug aaggaaucuu gagcgaccuc gauuuuaaua agggcgccau acugaaccug      300

ccuaauaacu gcugggagcu cagggugaag gguuuagaca aaaagcuguc cgauaaagag      360

ugggcagccg uucuuuugca uaucauuaag caccgggggu aucugucuca gcgcaaacau      420

gaccgaaaga cggaugacaa ggaacuagga gcucuccugu cgggcguguc aaagaaucac      480

agccuucugg aggaaggcga guucagaacc gcagccgagu uggcuaucaa cgucuuuugu      540

aauaguaagg ggcauauuag gaaccagugg ggagauuacu cccacacauu cucucggcuc      600

gaccugcaga aagaaaucaa gcaguuauuu gugagccaac gugagcuggg uaauccauau      660

acuuccucaa guuucgaaga gaacauaauu agccuccuua ugaaacagaa ggaagccauc      720

ucuggcaaug auauucugaa aauguugggg aagugcaccu acgagaagaa cgaguauaaa      780

gcggcaaaga auacauacuc cgccgaacgc uuuguauggc uaaccaaacu gaacaaccuc      840

agaaucguug acgacggagu ggagagggcu cuaaauucaa gcgaacggaa acuucugauc      900

gaugagccgu auaaaaaggc caaguugacu uacgcacagg uccgaaaaau ucucgaacug      960

cccgacagug cuguguucaa gggcgugcgc uacuuugccg auaacgcgga gucuuccaca     1020

uuaauggaga ugaaauucug gcacagcaua agaaaggccc uggaaucgaa uaaccuaaaa     1080

gacgaguggc ucguccugaa gaccaagccu gaacuuuugg augagaucgg uacgggguuu     1140

ucuauuuaua aaacugacga ugacaucacc uccuaccugu caaauaagcu cgauaaaaag     1200

guucuggaca uacuucuggu ggaaguaagu uucgauaaau uuauugaguu gagccucaag     1260

ugucugagga agaucauccc auuaauggag aaaggauauc gguacgacga agcauauaag     1320

uacauuuaug gcgaggugaa caucgaauac aaucugaacg acauuaaaaa ucccgucgug     1380

cuacgcuccu ucucucagag cagaaagguu auaaacgcug ugaucaggcu cuacggcagu     1440

ccugcccgua uucauaucga gacagcucgg gauaucggga aaucauucaa ggagcgaaaa     1500

gccauugaaa agcaacagga gaagaauaga cuggaaaaag ucagggcagc cgagcacuuu     1560

aagcagcuuu ucccaaacua ugugggagaa cccaaagcua aggacauauu gaaaguacgc     1620

cuguacgagc agcaacaggg uaagugccuc uauuccggca agauccugga uauuaaacgg     1680

uuaauggagc auggguacgu ugaaaucgac cacgcgcugc cuuuuagcag aacuugggau     1740

gacucuuuca auaacaaggu gcuugucuug gcaaaugaga accagaacaa aggaaauaag     1800

accccauaug aaugguuuaa cggcaaagau aauucagaca guuggaagag guuccucgcc     1860

cuggugaaca auagcgguuu uuccgcuguc aagaaacaac acauucuaau gaaggagauc     1920

gaugaagagg gguucaaaca gcgcaaccug aaugacacac gauacauaac ccgguuucuc     1980

uguaacuggg uggagaauaa caucccgcug cuuggcaagg gaguuaaacg uguguucgca     2040

ccccagggca agauuacggc cuugcugaga ucuaauuggg ggaucaggaa gguacgcgaa     2100

gauaacgacc ggcaucacgc cauugaugcu aucgucgugg ccugcacuac aguuucgaua     2160

cagcaaagaa uuaccaauua ugugaaaaac agcgagauca auaggaagau uaaacgacag     2220

aacuacuuaa aguuuccuga accauggaaa uucuuucgcg aggacaucuc cauacggguc     2280

uucucaaaca aucccaucga ggaacucaag cagcagcuga gugacagacc ugaggcaaac     2340

caucaauucg ugacuccacu uuuuguaucu agggcgcccg ugcguaaaau gacaggacag     2400

ggucacgaag auaccauucg cagcgcuaag cggaucuccg agggcauuuc aguucugaga     2460

gucccguuga cgaagcucaa ugacaaaagc cuggaacuaa uggugaacag ggagcgagag     2520

aaggaacugu acgaugcccu caaacagcgg cugcuugagu ucaagaauga cccugaaaaa     2580

gcauuugccg agcccuucua uaagaaggga ggacagcaag ugaaaaccgu ccgcaucgaa     2640

agaguucaga agaacggcgu guuggugaau aacaauacag guauagcuga uaacgggaau     2700

auuguaaggg ucgacguguu ucugaaaaag aaaaaguacu ucuuaguucc aaucuauucu     2760

uggcaagugg ccaagggcau ccucccuaac aaagcaauug ucgcucauaa gaaugagugu     2820

gauugggccg acauggaugg auccgcgcag uuucuguuca gucuuuacac uaacgaccug     2880

aucgaggugg uucuaaaaga uagcaccauu cucggcuauu uuucuuccau agaccgggcc     2940

acagcugcaa ucauuaucaa ggaacccgau auggagaaau caaaggggaa ggaagggauu     3000

caccgagugg guguaaaaac ugccagaagu uucaucaagu acaaugucga cgugcugggc     3060

aaaaacauaa gccgcugcag gaaggcuaaa cguuugccag ugcgguga                  3108


<210>  2610
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2610
augcugagaa agaugcacua caggcucgcc cuugaccugg gcagcaccuc cuuggggugg       60

gcuaugaucc gguuaaacgc aggaaaugag cccugcgccg ugauuaaagc ggguguccgc      120

auauucucua acggccgaaa uccuaaggau gggucaaguc uggcuguuac acguagagaa      180

gccagggcaa ugcggcgcag aagggaccgg cuacucaaac gcaaggcccg aaugauccag      240

acucugauug aguauggauu uuucccagcu aaagaagauc aaagaaaggc acuugagauc      300

augaacccgu uugcccugag ggcuaagggc uugaaugaag cccucacgcc cgcggaguuc      360

gcacgggccc uguuucauau uaaccagcgu cgcgguuuca aaagcaauag aaagaccgac      420

aaaaaggaua acgacuccgg ggcucugaaa acagccauca auaagcucag ggagacuuua      480

aagaccgaaa acugucggac agugggagag uggcugaaua aacgagaugu agcaggccuu      540

accgugcgcg cuagauacca ccaggacaag acuauaaaag aagauggcaa gucuaaaauc      600

aacaaguauu acgacuugua uauugauagg gccaugaucg agcaugaguu ugacgaacug      660

uggaaaaagc aggcggagcu aaauccuaca cucuucaccg aagccgcacg ggcugaucug      720

aaggacgucc uucugcacca acgcgccuug aaaccaguga agcccgggag augcacguuu      780

augccugacg aggaaagggc uccacucgcc cugcccucga cucagcguuu ccggauguac      840

caggagguua acaauuuacg aauacugaga gauggacuca aagaggaauc acuuacccug      900

aaccagaggg acgauuugau cgcagcccua gaggaaaaca auaagcgcac auuuacucaa      960

auuaaaaagc ugcucggucu gggcggggug gucaaguuca acuuugagga cccuaaacgg     1020

gaggaacuua agggaaauac cacaagcgcu auucugggca aagaugagca uuucgguagu     1080

gugugguuca aguuugacga agcgaaacag gaugcaaucg uaaugcaguu guucaaggag     1140

gaaaacgagg ccaagcuccu gagaugguua cagaaugaaa ccgggauuga cgagaaaagg     1200

gcugagacga ucgccaacgu uggacugcca gaaggcuaug gcucccuaug ugcacucgcc     1260

cuggcucgca uccuuccgga guugcgacgg gauguggucc ccuacucuga cgccgcaaag     1320

gcggcugggu uucaccacag cgauacuucc aauaaccaag aagugccugg acugacauuc     1380

ccaauugagc ucguggucac cgagacuggu gaaauaaaaa caaagcauau cuuucacgag     1440

cugcccuacu auggcgaaua ccuucagcgu cauguugggu ucggaucagg caaaccugag     1500

gacagugccg aaaagagaua ugguaagauu gcaaauccaa ccgugcacau cgggcugaac     1560

cagguaaggu ugguggucaa uacgcucaua aaacgcuacg gccaccccag cgaggugauu     1620

guugaggugg cccgggaccu gaagcaaucu aaagauuuaa gagacgaaga gggaaagagg     1680

caggcugaaa accagaaacg aaauaagcgc auccggaccg ccaucgcagg cguccugcag     1740

acauccgagg aaagagugag gcgugaugac auugagaaaa ugauccuaug ggaggaacuc     1800

ucauuugauc cggcugaccg ccggugcccu uauagcgggg uacaaauuag ugccgcgaug     1860

cugcuuucug augagguuga aauagagcau aucuugcccu ucucccagac ucuggacgau     1920

agccucaaca auaagaccgu ggcccugaga caggcuaaca gggucaaggg aaaccgaaca     1980

ccaugggaag cauuuggugc ccagucgacu gcuggcuucg acuacgccac cauuuuagca     2040

cgggcggagc acaugccuaa agggaagcgc uauagauuug ccgaggaugg auaccaaagg     2100

uggcugaaag acgaugcugg cuuccuuccc cgggccuuga augacacacg acauaugucu     2160

aagguggcaa gagaauacau gucccucauc uguccaaaca cccgcgucau ccccgguagg     2220

augacugcua ugcugcgugg gaaauuuggc cuaaaugacg ugcugggacu caacggcgag     2280

aagaaucgga acgaucacag acaucacgcc guugacgcau gcgugauugc cguaacggau     2340

caggggcugc uucagagguu cgcuaaggcg ucagccagug cacgcgaaca gcaauugaau     2400

cgacuggucg agaacaugcc uuuaccaugg gagagcuaua gagaacacgu ggagcgggcu     2460

auagacgcca ucuggguuuc ccauaaaccg gaucacucuc augaaggagc caugcacaau     2520

gacacagcuu acggucuccu gggcgauggg aaggugagca uucacaaaau ggucgaggga     2580

aagagggaac gcaucgagga gucacuuaaa gugauugcau ucaccaacgc caaggcgaau     2640

gaccgucaug gccuguugga uaacggugaa cccagaccuu auaaggggua caaaggcgac     2700

aguaauuauu guaucgagau aauccggaac gaaaagggaa aaugggaggg cgaaguaauu     2760

uccacuuuug augcuuacca gcucgugagg gagcacgggg uugcccgccu gcgacaucca     2820

acccuaagcg ucucuggaaa gccccuggug augagacuca ugaaagacga gacaauccgg     2880

auggugacca augaaaagcc uauuacugca agggucugcu ggguucgcuc agauacaaga     2940

aucgccuucg cuaguacgag cgaggcaaac guggacgcca gggaccggga uaagaacgac     3000

uccuuuucuu auauagugaa aaccgcgucg guacugcaga aacuucaggc ucgucgcauu     3060

agcaucuccc caacuggcga auugcgagau cccgguuuca aggaguga                  3108


<210>  2611
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2611
auggagcagc ugaacuacau ccucggccuu gacauuggga uagccagcgu gggaugggcu       60

gucguugaaa ucgaucacaa ugaggacccc auuagacuga ucgauguggg uguaaggacc      120

uucaacaagg cagaaguggc caaaacaggc gaguccuugu cuuuaauucg gcgccuggcg      180

cgagggucac guagacuggu caggcggaag aaaaugcgcc uccugacugc uaagagacuu      240

cugaaaagug aaggaaucuu gagcgaccuc gauuuuaaua agggcgccau acugaaccug      300

ccuaauaacu gcugggagcu cagggugaag gguuuagaca aaaagcuguc cgauaaagag      360

ugggcagccg uucuuuugca uaucauuaag caccgggggu aucugucuca gcgcaaacau      420

gaccgaaaga cggaugacaa ggaacuagga gcucuccugu cgggcguguc aaagaaucac      480

agccuucugg aggaaggcga guucagaacc gcagccgagu uggcuaucaa cgucuuuugu      540

aauaguaagg ggcauauuag gaaccagugg ggagauuacu cccacacauu cucucggcuc      600

gaccugcaga aagaaaucaa gcaguuauuu gugagccaac gugagcuggg uaauccauau      660

acuuccucaa guuucgaaga gaacauaauu agccuccuua ugaaacagaa ggaagccauc      720

ucuggcaaug auauucugaa aauguugggg aagugcaccu acgagaagaa cgaguauaaa      780

gcggcaaaga auacauacuc cgccgaacgc uuuguauggc uaaccaaacu gaacaaccuc      840

agaaucguug acgacggagu ggagagggcu cuaaauucaa gcgaacggaa acuucugauc      900

gaugagccgu auaaaaaggc caaguugacu uacgcacagg uccgaaaaau ucucgaacug      960

cccgacagug cuguguucaa gggcgugcgc uacuuugccg auaacgcgga gucuuccaca     1020

uuaauggaga ugaaauucug gcacagcaua agaaaggccc uggaaucgaa uaaccuaaaa     1080

gacgaguggc ucguccugaa gaccaagccu gaacuuuugg augagaucgg uacgggguuu     1140

ucuauuuaua aaacugacga ugacaucacc uccuaccugu caaauaagcu cgauaaaaag     1200

guucuggaca uacuucuggu ggaaguaagu uucgauaaau uuauugaguu gagccucaag     1260

ugucugagga agaucauccc auuaauggag aaaggauauc gguacgacga agcauauaag     1320

uacauuuaug gcgaggugaa caucgaauac aaucugaacg acauuaaaaa ucccgucgug     1380

cuacgcuccu ucucucagag cagaaagguu auaaacgcug ugaucaggcu cuacggcuca     1440

cccgcccgua uucauaucga gacagcucgg gauaucggga aaaguuucaa ggagcgaaaa     1500

gccauugaaa agcaacagga gaagaauaga cuggaaaaag ucagggcagc cgagcacuuu     1560

aagcagcuuu ucccaaacua ugugggagaa cccaaagcua aggacauauu gaaaguacgc     1620

cuguacgagc agcaacaggg uaagugccuc uauuccggca agauccugga uauuaaacgg     1680

uuaauggagc auggguacgu ugaaaucgac cacgcgcugc cuuuuagcag aacuugggau     1740

gacucuuuca auaacaaggu gcuugucuug gcaaaugaga accagaacaa aggaaauaag     1800

accccauaug aaugguuuaa cggcaaagau aauucagaca guuggaagag guuccucgcc     1860

cuggugaaca auagcgguuu uuccgcuguc aagaaacaac acauucuaau gaaggagauc     1920

gaugaagagg gguucaaaca gcgcaaccug aaugacacac gauacauaac ccgguuucuc     1980

uguaacuggg uggagaauaa caucccgcug cuuggcaagg gaguuaaacg uguguucgcc     2040

ccucagggca agauuacggc auugcugaga ucuaauuggg ggaucaggaa gguacgcgaa     2100

gauaacgacc ggcaucacgc cauugaugcu aucgucgugg ccugcacuac aguuucgaua     2160

cagcaaagaa uuaccaauua ugugaaaaac agcgagauca auaggaagau uaaacgacag     2220

aacuacuuaa aguuuccuga accauggaaa uucuuucgcg aggacaucuc cauacggguc     2280

uucucaaaca aucccaucga ggaacucaag cagcagcuga gugacagacc ugaggcaaac     2340

caucaauucg ugacuccacu uuuuguaucu agggcgcccg ugcguaaaau gacaggacag     2400

ggucacgaag auaccauucg cagcgcuaag cggaucuccg agggcauuuc aguucugaga     2460

gucccguuga cgaagcucaa ugacaaaagc cuggaacuaa uggugaacag ggagcgagag     2520

aaggaacugu acgaugcccu caaacagcgg cugcuugagu ucaagaauga cccugaaaaa     2580

gcauuugccg agcccuucua uaagaaaggg ggacagcaag ugaagaccgu ccgcaucgaa     2640

agaguucaga agaacggcgu guuggugaau aacaauacag guauagcuga uaacgggaau     2700

auuguaaggg ucgacguguu ucugaaaaag aaaaaguacu ucuuaguucc aaucuauucu     2760

uggcaagugg ccaagggcau ccucccuaac aaagcaauug ucgcucauaa gaaugagugu     2820

gauugggccg acauggaugg auccgcgcag uuucuguuca gucuuuacac uaacgaccug     2880

aucgaggugg uucuaaaaga uagcaccauu cucggcuauu uuucuuccau agaccgggcc     2940

acagcugcac ugaucauuaa ggaacccgau auggagaaau caaaggggaa ggaaggaauc     3000

caccgagugg guguaaaaac ugccagaagu uucauuaagu acaaugucga cguguugggc     3060

aaaaacauca gccgcugcag gaaggcuaaa cgucugccag ugcgguga                  3108


<210>  2612
<211>  3111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2612
augagaaucu ucggcuuuga cauugggaua accagcaucg gaugggccua cguggagucc       60

aacgaacuga aggauugcgg ugucaggauu uucacaaaag cugagaaucc caagaacggc      120

aaaucucucg cacuuccucg gcgcgaagcc cgaggggcgc guagaaggcu ggcucggcgc      180

aaggccagau ugaaugcaau caaaagguua cuguguaagg aguuuggacu aaacguuuca      240

gacuauuucg ccaaugaugg cgaacuccca aaggcuuacg agacuacgaa agacaccaag      300

aguccguaug agcugcggag ccuugcacug gaacagaaau uggagcccaa ggaacucgcc      360

cgcgugauuc ugcacaucgc uaaacaucga gguuacggga acaagcacgc caagacagga      420

uccgauaaag acucuggcaa gguacugucg gcgauaaaag ugaaugagac ugcaaugaag      480

gauaaauauc aaucagucgg cgaguaccuc uauaaggaau uuuaccagaa auuaagacug      540

gagagcgaaa agaaggggaa caauaucacc aaagaguuca agaacgugag gaauaaagga      600

gaaaacuacg agcauugcgu ugcccaggaa caggugcggg cugaacuuga guugauuuuu      660

gccaagcaaa aagacuucgg ugcaacauuu agugaaaauu ucgaguccca gaucauugaa      720

accgcuuuuu aucagcguga ucugaagucu uucgaggaca aggucggcaa auguguguuu      780

uacgagaacg aaaaucgcgc cccuaaggau agccuauccg cgauggaguu cguagcccuc      840

acuagaauca uaaacacacu gaaaaaccuu gaagagaagu caaagaaucu ggggauugga      900

gaaguuuaug gcaaggacaa gauccaggag auuuugaaaa ucgugcucga uaagggugag      960

augaguuaca aaaagcugag ggaaaucuua caccuggacg agcauauucu cuucgcaaaa     1020

gacagcaaac uugauuauac ccaagggguc caggaagcug agaaggccaa auuuauagag     1080

cugaagaacu ugaaggcuuu caagaaagcc augggaggcg acuuuucuaa uuucgcacgg     1140

gaggagcuag auaagaucgc cacggacauu acucugguga aauccaagga aaaccucgcu     1200

aagaaacugc agaauuacuc agcgcuuagc aaggaacagg uggaagcacu gaguaacuug     1260

ucuuuugcca aacacaucga ucucucccug aaggcuuuag agcaaauacu gccauucaug     1320

cgaggcgaca aaaccgggaa gugccuacgc uaugaugagg ccgucaagaa agcagaacuc     1380

caggagcaca gaaagcauaa acagaaggga gaauuucuga uuccccuuaa agacuacgag     1440

ccuuacuugg ccaauccagu uguggcuagg gcccugagcg aauaucggaa gguacucaac     1500

gcacugcuuc gcaaguacgg uaaagugcac aagauccauc uggaguucac aagagaggcg     1560

aaauugucgg cuaaggaaag gcaaaaauau gagaaggaac agaaggagca cuuugaagcc     1620

aaucagcgug caauccagca augugagauu cucggccugc ccaucaacgg gacuaauauu     1680

uuaaaaauga agcuguggcu agagcaggga gaaugcugug ccuacucugg cgagaaaaua     1740

acccgggaac accuccugga uuccaacgcc cuucaggucg accauaucua uccuuacuca     1800

cgaaguuucg augacagcua uaugaauaag guguugguuu uugcuaaaga gaaccaggac     1860

aaggguaaua aaacaccauu cgaggcauuu gggggcgaua aggaaaagug ggacaggauu     1920

cugucccucu cugcuaaacu gccgaagcaa aaacagagac gcaucagcaa caccaacuuc     1980

aaggauaaag agggaggcuu cuuagcccgg aaucugaacg acacggggua caucgcgaaa     2040

cuugccucac aguggacuaa ggauugcuug gaauuugagg accucagaga aaaugaggug     2100

acaauugcug gagagaaagg uaguuccguc cacauagaag ugguaagcgg ccagcugacc     2160

ucuaugcuaa ggcauuacug ggggcuggga aacaaggauc gcaauaacca ccuccaucac     2220

gcaguugacg ccgugauccu ggcuuauacu aauaacgcca ugauuaaagc auucgcgaau     2280

uuucgaaaga accaagagga aaauaaaaag aaguucuacg ccaaacagau cgcugaguca     2340

gaauauaagg ccaaaaaggc auuuuucgag cccuguaaaa acuuucggca gcgugucgag     2400

gaaaagauua aggagaucuu cgugaguaaa ccuccaagaa agaggguccg cggcgcccuu     2460

caccaggaaa cauuuuaccc cuucgagaaa uugugcaaug auaaggaaua uagcgagaaa     2520

cugaagaugg acuacggugg ggagaagggc augcaaaaag cuauagcauu aggaaagauc     2580

cggcagauug gcaccaaaau cguguccaac ggggccaugg uuagagugga uauuuuuaag     2640

gacaaaaagg gaaaauucua ugcuguaccu aucuacacga uggauuuugc gcucgguaua     2700

cugccaaaua aggccgucgc aauuggcaag gacaaagacg gggugaucaa ggauuggauu     2760

gaaauggacu cuaacuacga uuucuguuuc ucgcuuuuua aagacgaucu gaucuugguu     2820

cagaagaaag agauggaaag ggcugagcuc ugcuauuucg ugagcuuugg auccaccaac     2880

gcccaaauaa augucgagaa gcauaacaau aaguucgaag gccugucaga gaacgagaaa     2940

auccuauaca guaaugccac acagaaggag guggcuggua aaucuauugg gauccagaac     3000

cugaagacuu uugaaaaaua ucagguaagc ccccucggcg aggugcaaaa ggcagaguuc     3060

gaaccgcacc aggagauuaa gcugaaaucc ucaccuaagc gaaaagacug a              3111


<210>  2613
<211>  3111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2613
augcugcaca agaugcauua cagacucgcc cuugaccugg gcagcaccuc cuuggggugg       60

gcuaugauca gguuagaugc agacaaccag cccugcgccg ugauuaaagc gggaguccgg      120

auauucucua auggucgcaa cccuaaggau ggcucaaguc uggcuguuac acgacgugag      180

gccagagcaa ugaggcggcg cagagacagg cuacucaaac ggaaggcccg caugaugcga      240

acucugaucg aauauggguu uuucccagcu gaggcagccc aaagaaaagc ucuugaaacg      300

auuaauccgu uugcccugag ggcgaaggga uuggaugagg cacucacccc cgccgaguuc      360

ggccgggcuc uguuucacau caaccagcgu cgcgguuuca agagcaauag aaaaacagac      420

aagaaagaua acgacuccgg ggcccugaag acugcaauug gaaaagugag ggcuaccauc      480

gaagccgagg gcugucggac aguaggcgaa uggcuccaua agcgagaugu ggcgggguua      540

accguccgcg ccagauacca ccaggacaag gugauaaaag aggauggaaa gacuaaaauc      600

gacaaguauu acgaucugua uauugacagg gcaaugaucg aacaggaguu ugacgagcuu      660

uggaaaaagc aagcugaauu gaauccugcc cuguucacag aggcugcccg ggcagaucua      720

aaagacguuc uccugcauca gcgcccacuu aagcccguga agucugguag augcaccuuu      780

augccugaug aagagagggc cccacuggcu uugcccucga cgcagcguuu ccggauguac      840

caggaaguca acaaucuccg aauccugaga gacggcuuaa aagaggagcc ucugacucuc      900

aagcaaaggg augaccuuau ugcggcacug gagaaaaacu caaagcacag cuuuacccag      960

auaaaaacau ugcuaggggu gggaggcgcc guacaguuca auuuugaaga ucugaagcgc     1020

caggaacuca aggguaacac uaccagugcu auucugucca augacaaaca cuucgggaag     1080

gccugguuca cauuugauga ggcaaaacaa gacgccaucg uucuucagcu ggugaaggaa     1140

gagaacgagg cuaaauuggu ccgguggcuc caggaugaaa ccggaauuga cgagaagaga     1200

gccgaagcaa ucgcgaaugu gggccugcca gagggcuaug ggucuuuaug ugcuaaggcc     1260

cuggcaagga uccuaccgga acuccgccga gacgugauga cguacgauaa agccguccaa     1320

gcugccggau ucgagcauca cagccggcug gguuccaaca cugagauucc cggccuuaca     1380

uuuaagauag aaucaaucga ccaggauagu ggggagguua aacaguucca uauucacaag     1440

guguugccuu acuauggaga auaccugcag cgucacguag gcuuugguag cggggagcca     1500

gaggaccccg cagagaaaag auauggcaag aucgcuaacc cuaccgugca uauaggacuc     1560

aaucaaauua ggcuggucgu gaacgcccuu cugaagcgcu acggccaccc aucugaaguu     1620

aucguggaau uggcgcggga ucucaaacag uccaaggacc agagagauga ggaaucaaaa     1680

aggcaggccg agaaucaaaa gcgaaacaaa cgccugcggg acgcuaucgc agggacuuua     1740

aagauuagcg aggaaagagu caggcgugau gacauccaga aaaugauucu gugggaggaa     1800

cuaaguuucg aucccgccga ccgccggugc ccguauucug gagcucagau auccgccaca     1860

augcuccuga gcgaugaggu ggaaguagag cauauccuuc cuuuuucgca gaccuuggac     1920

gacucucuga auaacaagac gguugcacuc agacaagcga auaggauuaa ggguaaccga     1980

acccccuggg augccuucgg cgcucaggcc guggcagggu uugacuacga guccauccug     2040

guccgggcug aaggaaugcc aaaagccaag cgcuauagau ucgcagagaa uggcuaccag     2100

aggugguuaa aagaugacgc cucauuucug gcucgggcgc uuaacgauac acgacacaug     2160

aguaaggugg ccuucgaaua cuugaaacuc aucuguccua auacuagagu cauucccggu     2220

cgcaugaccg caaugcugag ggcuaaguuu gggcuaaacg acgugcuggg ccucaaugga     2280

gagaagaacc guaaugauca ucggcaccac gccguugacg ccugcgugau agcuguaaca     2340

gaucagggcc ugcuucaaag auucgcaaaa gccagcgcgu ccgcuaggga acagcaguug     2400

aaccgccugg ucgagaacau gccauuaccc ugggggucuu aucgagagca ugugcaaaga     2460

gccaucaaug caauuuggau cagccacaag ccugaccauu cacacgaagg agccaugcac     2520

aacgauacug cuuacggucu ccggggcgac gggaaaguua guuuccauaa gaugguggag     2580

ggagucagga cccacuccga agauaaucug aaagugauug agaucagcaa cgcaaaggcc     2640

acagaccgcc auggccuucu gccaaauggu gagccgcguc ccuauaaggg guacaaaggc     2700

gacucuaacu auuguauaga aaucguaaga aaugagaagg gaaaauggga aggcgaggug     2760

auuucaaccu uugaagcgua ccaguugguu cgggagcacg ggaucgcuag gcuccgccau     2820

aguacucuga gcgucuccgg aaagcuacug gugaugcgac ucaugauuga ugacgugguc     2880

agacugauca uagaugagca gacgcggaca augaggauug ccacccuuuc uggcaacggu     2940

cagaucuuca uggcagacac uucggaagcc aauguugaug cucgcaacag aagcaaagag     3000

gaaaccuuug ccuauguguc caagauggca gggucauugc aaaaggcuaa ugccaggcgg     3060

auuaacauca guccuauagg agagcugcgu gacccaggcu ucaaagaaug a              3111


<210>  2614
<211>  3114
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2614
auggagagaa ucuucggcuu ugacauuggg accacaagca uaggauucuc cgugaucgau       60

uacucuucaa cucagagugc cgguaacauu caaaggcugg gcguccggau cuuucccgaa      120

gcucgcgacc cugaugggac gccacucaau cagcagcgac gucagaagag aaugaugagg      180

cggcaacuuc gcagaaggcg gauucgccga aaagcacuga acgagaccuu gcacgaagcc      240

ggauucuuac cggcguaugg cagcgcugac uggcccguug ugauggccga ugagccuuac      300

gaacugagaa ggcggggucu agaggagggg cucuccgcau augaauuugg acgugccauc      360

uaccaucugg cucagcaccg ccauuucaag ggcagagagc uugaagaguc ugacacacca      420

gaucccgacg uagaugacga gaaagaagca gccaaugaga gggcugccac ucugaaggcg      480

uugaaaaacg aacagaccac acucggcgca uggcuggccc ggcgaccucc aucggaucgc      540

aagagaggga uacacgcuca uaggaaugug gucgccgagg aauuugagcg gcugugggag      600

gugcagucaa aguuccaccc cgcacucaaa agcgaagaga ugcgcgcuag aaucagugac      660

accauuuuug cccaaaggcc uguuuucugg cguaagaaca cuuuaggaga augccgguuu      720

augccaggug agccgcugug ucccaaaggc uccuggcuuu cucagcagcg aagaauguug      780

gaaaagcuga auaaccuagc gaucgccggg ggaaaugcaa ggccucucga ugcugaggag      840

cgcgacgcca uucugagcaa acuucagcaa caggcuucca ugucauggcc aggcgugcgg      900

agugcccuga aggcauugua uaagcagaga ggugaacccg gggccgagaa aagccucaag      960

uucaaccugg aauuaggagg cgagucuaaa cugcucggca augcucuuga agcgaagcug     1020

gcagacaugu uugggccuga uuggccagcc caccccagga aacaagagau ccgccaugcu     1080

guccacgagc gauugugggc cgcagacuac ggagaaacac cggauaagaa acgggugaua     1140

auucuauccg agaaggaccg uaaggcccau agagaagcug ccgcaaacuc auucguagcg     1200

gauuucggua ucaccggcga gcaggcugcc cagcugcagg cacucaaacu gccuacgggg     1260

ugggagcccu auagcauucc agcccuuaau cuguuuuugg cugaacucga gaagggagaa     1320

agguucggcg cccugguuaa cgguccugac ugggaggggu ggcgccggac uaauuuuccc     1380

cacagaaacc aaccaaccgg cgaaaucuua gauaaacugc ccaguccugc aucuaaggag     1440

gagagggaac gaaucuccca gcuacgcaac ccaacagugg uccggacuca gaaugagcuc     1500

agaaaagugg ugaacaaucu gauuggacuu uacggcaagc cggacaggau acguaucgaa     1560

gucgggcgcg auguuggaaa gagcaaacgg gagagagaag agauucaguc ggguaucagg     1620

cgaaacgaga agcaacggaa aaaggcuacc gaagacuuga uuaaaaaugg cauagccaac     1680

cccucucgcg augacgugga gaaguggauc cuguggaagg aagggcagga gagaugcccu     1740

uacacaggag accagaucgg cuucaaugcg cucuuuaggg aaggucggua ugagguagag     1800

cacauuuggc cacgauccag aucauucgau aacagucccc gcaauaaaac ccuguguagg     1860

aaggacguga acaucgaaaa agggaaucgu augccuuuug aggccuucgg ccaugaugaa     1920

gaccggugga gcgcuauuca gauaagacuu caaggaaugg ucuccgcaaa gggcgggacg     1980

ggaaugucuc cagguaaagu gaagagguuu cuggccaaga cuaugcccga ggauuucgcu     2040

gcccgccagu ugaacgacac acgauacgca gcgaaacaga uccucgccca gcugaagaga     2100

uuauggccgg auaugggccc ugaggcucca guuaaagugg aagccgucac cgggcaagug     2160

acugcacagc ugcggaagcu auggacacuc aauaacauuc uggcugacga uggagagaaa     2220

accagggccg accaccgcca ucacgcaauc gaugcccuua cgguagcuug cacccauccc     2280

ggcaugacaa auaaguugag ccguuauugg cagcugagag acgauccucg ggcggaaaaa     2340

cccgcccuca cuccaccuug ggacaccauc agggcagacg cugagaaggc cguuucagaa     2400

auugugguca gucaccgcgu gcgaaagaaa gucuccgguc cccugcacaa ggagacaacu     2460

uacggggaua ccggcacaga cauaaaaacc aagagcggaa cuuauagaca auuugugacg     2520

cggaaaaaga ucgagucuuu aucaaagggc gaacuggaug agauuaggga cccacgcauc     2580

aaagaaauug uugccgcuca uguggcaggg agaggaggcg auccgaagaa agccuucccu     2640

cccuacccau guguaagucc cggugggccu gagaucagga agguccggcu uacaagcaaa     2700

cagcaguuga accucauggc gcagaccgga aacggcuaug cugaccuggg uuccaaucac     2760

cauauagcca ucuaccgucu accagauggc aaggcagacu uugaaauugu gucucuguuc     2820

gaugccucgc gccgacucgc ucaaagaaac cccaucguuc agaggacucg ggcagacggg     2880

gccagcuucg ugaugucccu ggcggcugga gaggccauua ugaucccuga gggcucaaag     2940

aaagggauau ggauugucca gggagugugg gcaaguggcc agguagugcu ugaacgcgau     3000

accgacgccg aucacucuac aaccacuaga ccgaugccaa aucccaucuu gaaggacgac     3060

gcuaaaaagg uuagcauuga uccuaucggu aggguccggc cauccaacga cuga           3114


<210>  2615
<211>  3117
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2615
auggccaaac aggugagaua caggcugagc cucgaccuug gcuccaccuc ucuggggugg       60

gcuaucuucc gguuggaugc agacgcccac cccacagcga uuauaaaggc uggaguccgc      120

aucuuuucag auggucgaaa cgccaaggac ggcaguagcu uagcaguuac ucguagagcc      180

gcuaggucca ugcggcgcag aagggaucgg cugcuacgcc gaaaaacgag aaugcucgac      240

gcacugcuug aguauggguu cuuuccucaa gaugccgcuc agaggaaggc ccuggaacgg      300

uugaauccau acgcgcuccg ugcagaggga cuggcccgcc cgcugacccc cggcgaguuc      360

gcuagagcca uuuuucaucu caaccagagg cgggguuuca aaucuaaucg aaagacagac      420

aagaacgaua augacucggg ggcauuaaaa acugcuaccu cacagcugcg ccaagauauc      480

caggccagcg gaugcagaac agugggcgag uggcuuugga cccagagguu gcagaagaaa      540

ccugaaggcg uaccacacca aggggugcgg gcgcgcuaua gagagacucc ccauauuaac      600

agugagggaa agaaaaggau cgaaaagaag uacgaccugu auauagaucg uuccaugauc      660

gaggccgaau uugacgcacu auggucugcu caggccacau ucaauccugu cguguuucac      720

gcuaccgccc augagaaacu caaggacauu cugcuuuacc agcggccacu gcgacccguu      780

aaaccuggua gauguacguu gcucccagag gaagagaggg caccgcuggc cuuacccagc      840

acucagcgcu uccggaucua ucaagaacug aacaaucuca gacuucuggu ggaucaguug      900

aacgagcagc cucuaacccu ggcucaaagg gacacaauug cgacucucau ggaaggccgc      960

gcaaagaucu ccuuugauca gauacgaaaa gcccugaagu uagggucaau gaccaaauuc     1020

aaucuggagg acgauaagcg gaacgaguug aagggaaaug cuacaggcag ucagcucgcc     1080

aaaaaggaau uuuucggucc agcauggcac caguucgacg aggcccugca agaugacauu     1140

gucuggcacu uacugagcga agagaacgaa ucugcucuaa ucuccuggcu ccaggaucgu     1200

accgggauug acgccgcaac ggcgcaggcu gugucaaaug ccagccugcc cgagggauac     1260

ggcagucuuu cuagaaaagc auuggaggua auccugccua ggcuccgcuc cgaaguuauc     1320

acuuacgaua aggccgugca ggcugccggc uuuagccauc acucgcaacu gggguucgag     1380

uaugacccag acgaugucga agacuggauu gaucaugcaa caggagaggu gaaacccaua     1440

uuuaagcggc uuccguacua ugguaaggcu cugcagagac acguggccuu cggcucuggg     1500

aaaccugaag acucccagga gaagagguac ggaaaaaucg cgaaccccac cguccacauu     1560

ggcuugaacc agguucgagu gguagccaau gcucucaucg cacgcuaugg ucggccaacu     1620

gagauaauua ucgaacuggc cagagauuua aagcaaucaa gggagcagcg ucagcaggac     1680

caacgccggc aggcugauaa ccagaaaaga aaugccagga uccgaaacag uauugcacaa     1740

gcgcugggga ucgccgaagc ucgggugcgc agagaggaca uucagaagug gauacuaugg     1800

gaggaacucg auccuggcga cgccaugcau aggugcuguc ccuacagcgg agucccaauc     1860

uccgcaucua ugcugcuuag cgaacaggug gaaauugagc acaucuugcc cuuuucaaug     1920

acacuggaug acagucucaa uaaccggacc guuucccugc gacaggcuaa uagaaucaag     1980

cgcaacagga gcccuuggga ugcccgugaa gacuucgagg cacaaggcug gcauuaugac     2040

gggauuuuac agcgggccgg acugaugcca gugcagaaaa gauacagguu ugcugcggau     2100

gguuaccagc gcuggcuucg agacgaucaa gacuucuugg ccagagcacu caaugauacg     2160

cgguaucugu cuaggauagc ugccgaguac cuacgccugg ucugcccgca gggcacccgu     2220

gugauccccg ggcagcucac agcccugcuu agacggaggu uuggauugaa cgacauucug     2280

ggcuuagaag gugagaagaa ucgcaacgau caccgacauc acgcuguaga cgcauguguu     2340

aucgggauua cugaucacgg ccucaugcag agauucgccc gggcgucagc ucaagccagg     2400

gaacagggac ugacccgccu ugcagaggaa augccucugc cauggcccac auauagagac     2460

cauguggaua gggccgucag uuugaucugg gugagccaca aaccugacca uggccacgag     2520

ggggcuauga uggaggaaac ugcauacgga cucaugaagg augguaccau acggcagcgu     2580

cacaaagccg acggcacaaa ggggcgcgag gucgcgaaug ugauccgaau ugcugaacca     2640

gcccaaccca ccagacaugg aguugacgau cagggcaggc cgcugccuua uaaggguuac     2700

aucgggggcu ccaacuauug cauugagauc acucggggag cacagggcaa augggaaggg     2760

acggugauau cuacaucgga cgccuacgga auuguacgcg cuagaggcau cgccggucua     2820

aggaauacca gccaggggca aaacggacac ccacugguca ugcggcucgu gauuggcgau     2880

ucccugcgac uuagaaucaa cgacgcauug cagacuaugc gcguuaccaa gauaucaggu     2940

aauggccagg uguuuauggc ugggguccau gaggccaacg uggaugcgag gaaucgggac     3000

aaaagucagg ccuucgcacu gguaucuaag auggcuggaa gccuucaagc cgcucaggca     3060

cgucaggcca caaucucccc cauuggcgag aucagagauc cuggguuucc cggauga        3117


<210>  2616
<211>  3120
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2616
augaagaaaa gaauccuggg ccucgacauu gggauagcca gcgugggaug ggcucuuauc       60

gaguucgaug acgaauuuca ggauaacaag aaaaagggua ccauuaucaa auccggcguc      120

aggauuuuca cacgggcaga gacucccaag gaacgcgagc cucuggccaa gccacgacgu      180

gaagcgagau cucaaaggcg gcgcaucuca ugcagaguug agagguugaa uuuugugcgg      240

gaguuauucg ugaaaaacgg gcugguagcu aaggaaagug ucaauaaaaa caauccgaac      300

aauauauaca ucacgcuaaa ccagauuaag acccccuggc agcuccgcau ggaggcccug      360

gaccgaaaac uuacaaauga agaguuugca gugguucuga cucacuuggc caagcauaga      420

ggauauaaga gccucaggaa aaccgauucc aaggacaaca aaacaggcga gaugcugaag      480

gcuaucaauc agaacaaaga acggcuggau aauucugacu accguacccu cggugagaug      540

uucuguaagc acgaaucgua uaaagagcau aagcgcaaca aggaugggga auacauuaau      600

ucagugacua gagaguuaau cgaggaagag gcagagaaaa uauuugccaa gcaaaggaaa      660

gaaggaaaca acuucgcuga cgucgauauu gaacgggagu aucugaagau cgccuuuagc      720

cagaaaccua caggcaguau uaauaagaug gugggcuucu gcaccuuuga gaacggggaa      780

aagcgagcgc cacgcgacuc cuacucugca gagcuuuuca aaguauugaa uacgaucaac      840

aauacuacca ucacagauau gaagacugga gaaaccagaa acuuuacagg ugccgagauu      900

aaaaagauaa ucgaaauuau caaagacacc aagggcauaa cguauguuaa gauuaaaaag      960

gagcugaaac uagggagcuc ccaguucuca ggaguggacu acagggagaa ugcuaagagu     1020

aaaggcgaag gugagaucuu cgucagcaug aagacuuacc acgaaaucaa gucuaaaauu     1080

gagaagcucu ccaaagagaa guggaacaaa cugaaggaaa auacagagcu ucuggauuca     1140

aucgugaaca uuauagccaa uuuuaaagac gaugaaagca ucaagaagga guuccagaaa     1200

uugaagauug acgaagauau caucgacgug cuccugaacu uugaguucaa aaaguuuauu     1260

aauuuaaguu ucaaagcacu gcggaagaua auccccuuuc aauuccaggg gcagaaguau     1320

auuaccgcuu cuauguccgu cggauacagc cucaaacagg agggcaucaa gaacaaauuu     1380

cuucgcguuc cuucgcaaga agagaaugug aacguaccag uggucgccag agcguucgcc     1440

cagacuagga aggugauuaa cgcaguuauc cgugaauaug gccaguuuga ucaaauaaau     1500

aucgagcugg cuacagacuu gaaaaacucu aaguucgauc gguccaagau ugaaaaaggg     1560

cagaaggagu uucagucaca gaaagacaag guguuccaag agguccagga ucuaaccgga     1620

gaaaucauuc ccaguagcuc ccugcuccug aaauaccgac uuuggagaga gcaggacgaa     1680

agguguaucu auucuggucg caagauaagc cggaaggacc uguuggauac gggcuuccuc     1740

gacauugauc auaucauucc uuacucaaaa aguauggacg auggguuuaa uaacaaggug     1800

cugugcuuag ccgaggaaaa uagaaacaaa aagaaugaca ucccauucaa cuauuaccag     1860

aggauaggac gcgauuggaa uuuucugauc uccauuaucg cuagcaugaa aaacaugaag     1920

uacguuaaaa agaagcgacu acucaaacaa gagcugucug acauugagau cgaaucauuc     1980

auagagcgga auauuaacga uacccguuau gccacaagau acaucaagaa uuuucuugaa     2040

aacaauuugg aguucaaaga gaacgaauuu aucaaggcaa aaguacaggc caggcacggc     2100

ggucugacca gugugcuccg cuauaacugg gggauuagca agaaucggga ggaauccuac     2160

cugcaccaug cuaaggacgc gaucacuguc gcauguucua cacagggcau ggugcaguau     2220

auuucgaaca uaagccaaaa auacgagaau ggaaaguaua aaaugaagca gaucaaagaa     2280

agaacugaga acaggauucc gaagcccugg gauuccuuca aggacgaugu cgagcgauca     2340

aucaaugaca uuuuugugag uuucgccccu cgccacaaag uuaccggcuc ugcucauaag     2400

gaaacaaucu acagcaaaaa gcaccuugag aaaggguacg ugaccauaaa gaagucccug     2460

gacaaaauca aguugucaga ucucaaaaac auuccaugcg acgaaaauug ugauaucauu     2520

cggguacuga aggagagauu agaaaaauuu aacgacgaua gccugaaggc cuucgcagac     2580

cccguccaca ugccuacuaa gugcucuaau aaaaagggac caaucauaag guccgugaaa     2640

auuaaggaga acaauaaaac ggguaucgag guucguaagg gccuagugga acgcggggau     2700

augguccggg uggacguauu uaucaaagau aagaaguauu accucgugcc cauuuauguu     2760

agugacuuca aaaacaagga ucugccgaau aaagccauag ucagcucuaa guccgagaac     2820

gaauggauca ugauugacaa aacauacuca uucaaguuua gucuuuucaa ggaugacuug     2880

aucaaaauua agaauaaaaa gggagagauc uuuggcuauc uggguggggu gcauagacuc     2940

accggcggaa uaaacaucaa aagcuguuac aagccuaagg aaaaagacgg caacauuggg     3000

ucgaagugcc uguuagauuu caggaaauuu caggugggaa uccuggguga cuauaaugag     3060

gucaagcacg agaaacgaau gcccgcuuac auuaacacua agaaggauaa agaacauuga     3120


<210>  2617
<211>  3123
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2617
auggacagaa gggaugcccg ggcuaugcgc cgacguagag acagguucca gcaacggcag       60

cgcgcacugc ucaaguaccu uguggccgau ggccuguuuc ccgaggacga aggggagaga      120

aaagcguugg cugccuuaga uccuuaugca cugagggccc gggcucuaga cgaagcacuc      180

agccugcacg agcuuggacg cgcccuguuc cauuugaacc agcgaagagg uuuuaagucc      240

aauaggaaag cugaucggaa ggccgacgau gacgcgggca aaaucgcaau uggggucgau      300

cgugcccgcg aagcuauggc cgagaacgga gcaagaaccu acggcgagug gcuccacagg      360

cggcgagcuu cugccacaga cgcgaaugcc auaccaccgg uucgcacuag acugaggccc      420

gaaucaggug aggaugcaaa gggggacgga uaugacuucu acccuggccg ggaucugcuc      480

gaagaggagu uugcugccau cugggaagcu caggccgcac aucacccaga cguguuaacg      540

gaugacguau augagcgccu guucgaaauu guguuuuacc aaagaccccu uaaggccccu      600

cagaucggca ugugcaccuu gauugcuggg gauaggcguc ugccaaaagc gcauccccua      660

uuccagcggc gaagacuccu ggaggaacuu aacgcacuga ugaucgucag gccuggagag      720

guggcccagc gcuugacacg ggaggaaaga gacacucucu uucugaaguu aaaagauagg      780

aauaagguua guuucgagag ccugcgcaaa acccucaagc uagacccaga ugcucgauuu      840

aacaaggaau ccgagaaucg gacagaacug aaaggugacg agguggccgc agccaugggc      900

ucuaagaccc guuucgggaa cagauggguc cacuugucga uagaggaaca augggcugug      960

aucgagaggc uaggagaugu agaaucagac gccgaggacg cagcguuucg cgcuuggcug     1020

gccgaacggu augagcucac uccggagcag acaagagcag uuaccaaugc uccccugccu     1080

cagggcuacg guagguucgg ggaaacggcu acucgaaaac uuauugaugc ccugcgcgac     1140

ggagagaagg auggccgggu guuggucuac agcgaagcag ugaccgcugc cggccucggg     1200

caccauagug acagaaggac aggagaggug uucgaggaug aagagggucg uccacagcug     1260

cccuauuacg gcgucgcguu agaacgccac aucaugccug ggacuggaga cccauccgau     1320

ucugacgaga ugcggguugg cagacugacc aaccccacag ugcauauugg ucuaaaucaa     1380

cucaggcgaa ucguaaaccg gcugauacgc gccuaugggc cgccugcuga aauugcaauc     1440

gagcuugcca gagaguugaa acugaccgau gaagacaaga aaaggauuaa uaaggagaac     1500

aauagcaacc ggcucgaugc ugaacgaaga uccgagaagc ugcuugccau cggcaaaccc     1560

gacacgggag caaaccgcgc gcuguugaag cucugggagg aacugaaucc agccaacgug     1620

uuagauaggc guuguaucua cucaggcguc cagauuagua uagacaugcu guuuagcgcu     1680

gccguggagg uugaccacau ccuaccuuuc acugcaacac ucgaugacuc uaaugggaac     1740

aaaauugugu gcacccggga agcuaauaga gagaagagga aacgcucccc cuaugaagcc     1800

uggggacacu cagcacgaug ggaggaaauc gccgcuagag cggcccagcu gccacggaac     1860

aagagguggc gcuuugagcc cgaugcaaug gagaaguuca aagacgaugg uggcuuucaa     1920

gcucgucauc uuauagacac ucaguacuug gccagacuga gccgggaaua ucucgccaca     1980

cuguacccug auaaggggga gggaaguggc cacauuuggg ucucuccagg uagguuaacc     2040

gaaauggugc gccgaaaacu ggggcuuaau gacuugcucc cggaucauaa cuucggcgga     2100

ggcgcugacc aggcaaagaa uagacuggau caccggcauc acgccaucga cgcgguaguu     2160

guggcuaucg ucgauagggg gaugcuaaac cgcauugcca gagcauccgg acaggagggu     2220

gccgagggca gggaacggau cauuauaccc gagccuuggg aaggguuucg ugacgaccug     2280

cgcacggcug ugaaugcaau caccgucagc caccgaacag aucauggaac ugccucgaaa     2340

gcgggccucc caaaggguaa ggacgcuacc gccgggagac ugcacaacga uacagcauac     2400

ggcuucacug gagagucuga cgccaaaggc aauuccauug ugguuaggcg ggugccccuu     2460

ucaaguuuua agaaauuggc ugauaucgac cgcguaagag auccugaacu gaaggccgca     2520

uuauaucaac aucucgacgg gcuggaggga aaagccuucg agaaggccau cggcgaauuu     2580

gguagcaggg aguggcaccc auacggggga guccggcgag ugagaguuac cgaaccccuu     2640

uccgugauuc cgauacgcga uaggaacggc cacaucuaua aggguuacaa aggcgacucu     2700

aauuaucggu acgaugucug ggagcugccu gggggaaagu gggugaacga aguagugagc     2760

auguucgacg cgcaucagcc aggcuggcag ucagccauuc gugcagcuaa ccccacagcc     2820

agaaaaguuu ugagucucca cagggacgau guccuggcua ucgagcgcaa uggggcagag     2880

cgggaacuug ugcgaguggu caaguuuucc gagaaacagu ucgccguugc cccucccaac     2940

gaaggaggcg cucugaaggc gagagacgca gauaaaggug acccauucaa guauagcuac     3000

ccuucuccca auacccucaa ggcccugagg gcucgccaag ugcggauuga ugagcuuggg     3060

agaguguugg acccaggauu uccggcccgu acuaaacaga ggaagacgcg gaaaccuaac     3120

uga                                                                   3123


<210>  2618
<211>  3132
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2618
augcagagag acaacccccu gguguacagg cucgcccuug aucugggcag caccuccuug       60

gggugggcug ucuuccgguu agacgcagag ggaucuccuc uggccaucau uaaggcgggu      120

gcucgcauau uuucagaugg ccgaaaucca aaagacggga guagccuagc cguuacacgu      180

agagcagcca gggcuaugcg gcgcagaagg gaucggcucc ugaagcgcaa agcacgaaug      240

cuuggagccc uggaaagaca cggcuucuuu ccgcaagacg aguccgcuag gaagguguug      300

gaaacucuca accccuuccu gcugcgggcc cagggucucg agguagcguu aaaaccugaa      360

gaguuugggc gugcacuguu ccaucuuaau cagcgcagag gauuucaguc uaacaggaag      420

acggaucgga aggacucgga uucaggcgcc uugaaacaag cuaucggcac ccugcgagcc      480

cagaugcacg agagcgggug ccgcacagug ggagaauggu ucuggaaggu cagaauggag      540

cauaaaccag aaggugugaa gggccagggg guuagggcac gguaucgcga gacucccuuu      600

accacagacg aggggaaaaa gagaauugau aagagguacg accuauaugu ggaucgugcu      660

auggucgccc aggaguucga cgcgcucugg gccgcacaag cugccuuuaa uccugcucug      720

uucaacgaag ccgcacgggc cgaacuucga gacauccugu ugcaccagag aaaacucagg      780

ccagugcagc ccggccgcug uacccuggua ccugaggaac cacgggcuuu acuggcgcuc      840

aguuccacuc agagauuuag gauuuaccaa gagguuaauc aucuucgcgu gcugcacgau      900

gaguugcgag aagagacacu aacccuggca cagcgggacc agaucgucgc ccuccuggaa      960

aagcaagcua aagugacguu cacugccaug cguaaggcac uugguuucuc ugcccuguuu     1020

aacuuggagg augcuaaaag agacgggcuc aagggaaaug ccaccgcagc ggcucugagc     1080

aaaaaggauu uauucggccc gcugugggcc ggcuuuggga cagcacuaca ggacgaaaua     1140

gugacucagc ucaugaccga ggaguccgaa cccaggcuga ucgccuggcu ucaggagcac     1200

acaggagucg augaagcucg cgcccaagca auugcuaaca ccgguuugcc ugagggcuau     1260

gggucacugg gacggacggc ccucgcgaga aucguuccag aacugcagag ggacgugauu     1320

acuuacgaua aggccguaca ggcugcaggc uucgcccauc acagugaccu ugguuuuagc     1380

uucgagcaug gggcugauga gguggaacga aucggcgagc gcacaauagc cucuaccgga     1440

gaaauuaaac ccgucuuugc auucaagcag cugccuuauu acggccgggc guugcaaaga     1500

cacguggccu uugcuaaaga caauccaagg aacgacgagg agcguuacgg gaagaucgcc     1560

aaucccacug uucacauugg acucaaccag gugcgcgucg ugguaaaugc acugaugcgg     1620

agauauggua ggccggcuga aguugugauc gaguuagccc gagaucugaa acagucccgg     1680

gaacagaagc uagaggcaca acagcgccag gccgacaacc agaagagaaa cgcuaggauc     1740

cggcgaucaa uugcggaagu ccucggcaua agcgaggaga gagugcgcac acuggauauc     1800

caaaaaugga uucuuuggga agaguugagu caugacgccg cagauaggcg uugcccuuac     1860

ucuggggucc agaucuccgc uagcaugcug cucggagaac ggguggagau agaacacauu     1920

cugcccuucu cgaugaccuu agacgauucu cugaauaaca gaacgguugc ccuuagggag     1980

gccaaucgca ucaagcgaaa cagagcucca ugggaggcac gggccgacuu ugaagcgcag     2040

ggcugguccu augaugguau cuugcaaagg gcugagcgca ugccucuccg uaaaagauac     2100

cgguucgccc ccgacgggua ugaaaggugg cugggcgagg auaaggacuu ucuagcacgc     2160

gcccugaaug auacccgaua ccucucaaga guggcugcag aauaucugcg gcuuguaugu     2220

ccaggaagug ccacaagggu cauucccggc cagaugacug cguugcugcg cgggaaauuc     2280

ggauuaaacg acgugcucgg ucuggacggc gagaagaaua gaaacgauca uaggcaccau     2340

gcuguugacg ccugcgugau cggggucacc gaucagcgga ugaugcagcg uuucgcacaa     2400

gccaaugcuc aggcccgcga ggacggacuu acacgacugg uggaagauau gccuuugcca     2460

uggccgacuu acagagacca cguagagagg gcagugcacc auauuugggu uagccacaag     2520

cccgaucaug gcuuugaagg ugcuaugaug gaggagaccu ccuacgggau acuccgggac     2580

ggcucuauca aacagcgcag aaaggccgau ggaagcgcgg gcagggaaau uucaaaccug     2640

auccggaucg ccgagccugg gcagccacaa cgacacggag ucgacgcaga aggcagaccc     2700

cuaccuuaua aagguuacgu gggaggaagu aauuauugua uugagauaac agugaacgau     2760

aagggcaaau gggaagguca ggucaucucc accuucgacg cuuaccgcau uguuagggag     2820

ggcgggcugc ggcagcuccg ucacccaacu cagggacaaa auggcagagc ccuggcuaug     2880

aggcuuguga ucggggauag cgugcgcuug gaggcaggag aaggcucucu gcagacguua     2940

cggguagcca agaugucagc cgcuggucag gucuuuauga gugaggugaa ccaagcgaau     3000

guugacgcac gaaacagaaa cggggaccuc cccuauguga gcaagauggc cggauccuuc     3060

cagaaagcua gggcccgcca ggucacaauu ucuccgaucg gcgaacugcg ggauccuggu     3120

uuuaagggcu ga                                                         3132


<210>  2619
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2619
augaaguaca cccugggccu cgaccuuggg uauaacagca ucggaugggc cauucuggag       60

aaugaugaaa acgguucccc cauaaaauug uucaaugcug gcgugcacau cuuugagccu      120

ucuauugaag acaucgagau ugaugggaag ggaaaaacaa agaacgucaa aagaagggaa      180

gcacgguuac gccgacguca gcuguucaga aagacuaaga ggcuagagaa acuccugaac      240

uuuuucaucc aagagggcuu uuuccagaau cagaagguuc uuauaaauga cccauuugaa      300

cgggccgagu uccuguacca guuggauaaa aagucacgca acccguauau ccucagaagg      360

gaagcgcugg acaaucggcu gagucucgag gagauugcuc gcaucuuuua ccauauugcc      420

caacaccgag guuucaaaag cacgaagaga gaauuagcag agaugaacaa ggaugaaaag      480

aagaaaaagg aggacgggau cauacagucc gaaauuaaua cccugaaaga ggagaucaag      540

aaaaacucuu gcaggacacu uggagaauau uuugccaagu uguucgagaa uggcgaaaag      600

auucggggcc gucauacuga ucgcaaaaug uacgaggaag aguuucagaa gaucuucaac      660

aaacagaagg aguucuaucc caauaucuuu accgacgagu ucaaaaagaa gcuguacaaa      720

cuaauuuuuc accaaagaga gcucaagucg cagaaagaua aggugggguu cuguucacug      780

gaaaggggua aaaagcgggc uccucuugca cuguugguaa gccagcgaua ccgcuauaug      840

ugcaagguga acaaccucaa ugucauagac acagagaccg gagaaaacag agagcugacu      900

guggaugaga aaaagaaauu auacgaaaag cuggagaaua gugaaaaacu cuccuuuaag      960

gagaucaaga aaauucuuaa ccugucugag aagagguaua ucuucaauuu ggagaaaagc     1020

gaaaaguaca uaauuggcaa caaaacaaau guuaagauga aaaacaucau caaggacugg     1080

gacaauuuug augaagacaa gaaaaacuuc uuuauuaaug aggcccggac cguggucaag     1140

aucgaaacgc uagagcgcag agggaaaaag cugggacucu ccgaugagga aucaaaagag     1200

uucaguaagg ugguauuuga agacggcuau cugaacuuca gcaagaaagc uguugagaaa     1260

cuucugccau acuugaaaga agguaagaaa gaguaugagg ccauuaagcu cgcguacccc     1320

gaaaauucug ugcagaacga uaaucugaag gaguuagacu uucugccucc aauaaaaucc     1380

aaggaacuca aaaaguacuu cgaggucagg aaccccaucg uggaaaaagc acuaacugag     1440

acccguuauc uggugaacuu ccuuauuaag aaguacggga aaccugagac aaucaagauc     1500

gaauuggccc gggagcugaa acucucaaau aagcaaaaag aacgaaagca uaaagagaac     1560

caggagaaua gaaagaagag ggaagagguc auugaaaaac ugaagaacga ucuuaaaaua     1620

agcgagccaa cugaaaccca gauccaaaag uaucuguugg cugaggagug ugacugcaaa     1680

uguccguaca caggacgcag uauuucuaug gauucccucu uuggcgaaca cccccuguuc     1740

gacaucgagc acauuauccc uuuugaucgg agcuuagaca auucguuccu gaacaagacc     1800

cuaugcuauu cugaagagaa uagaaagguu aaacauaaca agacgccaua cgaagccuau     1860

uacggcacug agaaauggga ggcaauaauc gaaaggguga agaaauuuaa guccaaugua     1920

cgcaacgaga agcuccgacg guuccagaug acaaaagaag acguggagaa uuacauucag     1980

aacuuuucaa cccagaaucu gaacgauacu aaguaugcua guaaacuugc caagaaauac     2040

uuggggcugc ucuauggaag caucaaugaa gacgguauug auaagaaccg uaagagaagg     2100

aucuucgcgu ccucuggcaa agucacaagc acccugcgcu acgccuuagg gcugaacacg     2160

auacuuaaug acgagaacau uaagacccgg gaggaucaca gacaucacgc aaucgacgcu     2220

auuacaaucg ccuugacuga uucaaguaug aucaaaaaga uauccgacgc uagcaaaagg     2280

gaaaagaaua uuaacucucu cuuuaaggau uuccaagccc ccugggagaa uuuucgaaaa     2340

gaaguggagg agaucauugc aaagaugaac aucucaauac gcauuaauaa aaagguuagu     2400

ggacagaucc acgacgaaac cuucuauaaa aagccuuuua aagaggaaga aaagagcuac     2460

acacaucagc ggaucaaccu ggaggaacua acuauuaagg augaggacaa aaagaaacag     2520

uccucuaagc ugaucgauaa uauuguggac ucgaaaauaa gaaacaucau uaucaauagc     2580

cucaacggcg aaaagccaaa gauuagguuc aaagacccca aggaucaccc guauaucaua     2640

cagaaaaaug guaagaaaau ccaaauucau aaggucacca ucaagacaaa acuggagacc     2700

uacaacgaaa uugggccuga gaaggccaaa cguuacguga aguccucaga cagucaccau     2760

aucgaaauau ucgaggaucu uaaaacuaag aagugggacg gcuauguagu uucuuugcug     2820

gaggcuuacg aacgcaaaaa gaaaaaguua cccauuguga auaaaaaccu cggagagggc     2880

aagaaauuuc uguucagccu ugcgaagggg gaaaucaucg agcuggauac ggaaaacgga     2940

aagcgggagu uguuugucgu gagaauuaua uccgcaucaa auaaccagau caaauucaca     3000

aggauuaaug acagccgaaa gaucaccgau auuccagcca cuggucucac cgcuuguccu     3060

ucuucccuga aaaagcggaa cugcaaaaag gucgugauca auauacuagg cgagaagcac     3120

uauaguaacg acuga                                                      3135


<210>  2620
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2620
auggccgaga agaaauucaa guacagacug ggccucgacc uugggaccaa cagcaucgga       60

uggugcaugc ugagguugaa uaaagauaac gaacccgcug cagugauuaa guccgguguc      120

cggauauuuc acgacggccg caaugagaaa acaaaggaac cuuuagccgu ugugcgacgu      180

aacgcgagau cuaucaggcg gaaucuggau cgcaagauuu caagaaggaa ccaucuacuc      240

aauacucugg uaaaauauuu ccuucugcca aguaacgaga ucgaacggaa ggcuuuggcc      300

gugcucaauc cguacgagcu gcgcagccga gcagucaacg agagacugga acucuuugag      360

uuagggaggc ugcuuaugca cuuguccaaa cggcguggau ucaagucuaa ucgcaaagug      420

gacaaggaag auucgucagg caagauuaaa cccgccaucg agagacugaa gaacaaaaug      480

caggagacga auaagaaaac cgcuggugaa uaucuauaca gccucaugca aaacgggaag      540

ccuguuaggg cacggcuggg acgaauagac ggcaguaaug gcuaugagau cuaccuugau      600

cgcgcccuga uugaagagga auauaacuug aucaugaacg agcagaaaaa gcaucacaag      660

gagcucacag acgauauuau caaugaaaua uuuaacauua ucuucuacca gagaccacug      720

aaaaagcaga uuaucgggaa auguuccuua acuggagagg acaccaagcu gaaaaaggcu      780

caugugcucg cccaagauuu uauccuucug cagaaggucc aggacuugag gguguucgau      840

gacaaucagc uagaauuucg gccccugucu aacgacgaga aagauauuau aaagcgcgaa      900

cucaggaaaa agaaagaggu aagcuacgag aauaucagaa agcugcuuaa gaaauauuac      960

gaaaacguua aguaugguau guucucccac gagacacugu acaaaaaguu gcaaggcaau     1020

aaaaccaacg cgcucauguc agacaaggaa auuaucgggg auaguuggua uagccugucu     1080

ccugaggugc aguacgacau aauugaagag uuauauuccg auaaggacaa ugaugagcug     1140

ucaaaacuau acagcgaaaa guuuaaccuc aaugagaguc agaucaacgg acugcuuaau     1200

aaagcaaucu accguuugga cucuggcuau auucgguacg guaagacugc cgucacaaac     1260

cucaauucca ucauggauaa agacaaccug aaccugcacg augcucgaga agaggccggg     1320

uaugagaagg aaaauaaaca ggacgcaagc gaguaccuug aauauuacgg aaaggugcug     1380

ccagacucgg uggucgaucc ccaaauugag aacccuaaga augacgaaga gagauauggc     1440

aaaauagcua acccaaccgu ucaugccgcg uugaaucagc ucaggaagcu ggugaacgag     1500

uuaaucaaac uguacggcaa gccggaacag auuguaaucg agcuagcccg cgaucucaaa     1560

aauucucgga aggcaaagga cgugaucaac aaaaagauug cugaaaauag aaaacugaac     1620

gaggaagucg auaagacgcu ucaagaguac uccgagaaau auaauacuca cauagugaag     1680

aacaaguuga auagggacaa aguuaagcug uggcgcgaac ucgggaaaga uccccuggag     1740

cgaaagugca ucuacaccgg agaacagauu aacaucgcca aauuauucuc agacgaggug     1800

cagauugaac auaucguccc uuauaguaag acacuggaug acagcuuuaa uaacaagacu     1860

cuuuccauga aacaggcuaa cuacuauaag gguaauaaaa ccccauacga ggccuucaag     1920

gauucuaaag acggcuauaa cuacgaugac auaaucgcac gggccaaguu gcucaauaac     1980

gacaauaaau ucaagcguuu uacaaaggau gcuauggaca uuuacaacag agaugggaaa     2040

gacuucaucg cgaggcaacu gagcgauacc caguaucuau caacgcgcgc acugauuuac     2100

cuccagaguc uguaugagaa ggcccaccac uccagcaucu ggacuauacc cggacagcuu     2160

acagcuuuga uucggaauua ccugggcuua aacacccuca ucucuaauga caaaaacaag     2220

aauagaaacg aucauaggca ccaugccgug gacgcauuug uaguugccgu gacuucacga     2280

agucugcuuc agaaaauuag cuaugcugcc ucccugcaag aagauuugga caaucgcaac     2340

gucucuucgu accggaauaa gcuccuggag aagaugccug aaccauuccc caacuauaga     2400

aaaagccuag agcuggcaau cgauaacaua aagacauccc acaaagcgga caggucaauc     2460

agugguaagc uccaugagga uaccgcuuac gggauugugg aaaaugacga caaauacaac     2520

gucgugacgc guuggaccgu ugauaaguuu aaggagcaga aacacuucga aacaauccgc     2580

gacaaugagc ugaaggaaau ugccuguucu gauaaagagc uuuuugcaaa gaucguggag     2640

acucggaaaa uaaagagcau uagaaaguug gccaaagaaa acccgguaau caagaucaaa     2700

gacaaguccg gcagggauua uaaggcuuuc gccggaggca auaacauuug cgucgagauc     2760

uacgaagugg acgggguucg aaagucagag gugauucagc uguuugaugc agcucaaaag     2820

ggauucaugc cugaauggau gaaaaaguau cccaauggua aauuacucau gcggcuguuu     2880

aagggcgacg ucauaggguu caucgagaac cgcaaauaca auuauuacac cauuaaggga     2940

aucagcauug ccgaucagaa ccuuaaacug cuagcgauca auaaggccga gggcauauuu     3000

aagaaagguc ucaacauccu guucgacucu aauaacaaaa auuccuugaa cgcuaaacag     3060

uucaauauua gugugacagg gaucguaacu aagaaaaaga ccgcagaugu gaacuuuugg     3120

aagaacagaa aauga                                                      3135


<210>  2621
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2621
augcugcaca agaugagaua caggcucgcc cuugaccugg gcagcaccuc cuuggggugg       60

gcuauggugc gguuaaacgc aaaucagcaa cccugcgccg ucaucaaagc gggaguucgc      120

auuuucucua acggucgaaa uccuaaggau ggcucaaguc uggcugugac acguagagag      180

gccaggagca ugcggcgcag aagggaccgg cuacucaaac gcaaggcacg aaugaugaga      240

acucugauag aauauggguu uuucccagcc aacgagacgc agaggaaagc ucuugaaaau      300

cugaacccgu acgcauugcg ggccaaggga cucgaugagg cucugacccc cuccgaauuu      360

gcccgugcgc uguuccauau caaucagcgc agaggcuuua agucuaacag gaaaacagac      420

aagaaagauu cggacucagg ugcacucaag acugccauua aacaguuaca cagcguacug      480

gauccucaag ggaaugacgg aaagccacgg accgugggcg agcuuuugua uaagcgauuc      540

acagaucuga guaaacuacc caaggaccgc accgucagag cuagguaccg gcaggauaaa      600

aucgugaagg acgauggcaa aacuaagauu gacaaauauu acgaccucua uaucgaucgc      660

gccaugauag agcaggaauu ugacgcacug uggaagaagc aguccgagcu uaacccuauc      720

cuguucacag aaaaugcuag agccgauuug aaagacguuc uccuguacca aaggucuuua      780

aagccaguga aacccgggcg uuguaccuuu augagcgagg aggaacgggc gccucuggcc      840

cucccaucca cgcagcgauu cagaauguac caggagguca acaaucuuag gauucugcgc      900

gaaggauuga aggaggaauc acuaacucug cagcaacggg augaccucau caacgcacug      960

gagaaaaaua acagaaggac cuuuacacag auuaagaagc ugcugggugu gggcgggagu     1020

guacaguuca acuuugagga uccgaaacgc gaagaguuga agggaaauac uaccagcgcu     1080

auccucggca aaucugaaca uuucggugag gccugguucg cuuuuaacga agccaagcaa     1140

gacgcaauag uucugcaguu aauuaaagag gagaaugaag ccaagcuggu gcgauggcua     1200

caggaugaga cagggaucga cgaaaagcgg gcugagguca uugcgaacgc aggacucccc     1260

gaaggcuaug gcucccugug caucgaggcc cuugcucgua ucuugccuga gcugagaagg     1320

gaugugguga ccuacgacaa agccguccag gcagccgggu ucgaacacca uucaaagcuc     1380

aaucgcaacg aggaaauucc agacauaacg uuuaaaaucg agagcauuga ucggaauagu     1440

ggagagauca agcaauucca ccuucacaaa gaacuucccu auuacgguga guaucugcag     1500

agacauguug gcuuuggguc uggaaagccu gaagacucca uagagaagag guacggcaaa     1560

auugcuaacc caacugugca caucgguuug aaucagguac gaguggucgu gaacgcccuc     1620

aucaagcgcu augggcaucc cagcgaaauu aucguugagg uggcacggga ucugaaacag     1680

ucgaaggaca gaagggauga ggaaaauaaa cgucaagcgg agaaccagaa gcgcaaugaa     1740

cgguuaagaa aagacauugc ugauauacug ggcaucucug aggaaagggu ccgacgggac     1800

gauauugaga agaugauccu augggaggaa cucuccuucg acccggccga ucgcagaugu     1860

ccuuacucag gagugcaggu aaguacaguu augcugcuua gcgacgaggu ggaagucgag     1920

cacaucuugc ccuuuuccca gacccuggau gacucucuca acaauaagac uguggcacug     1980

aggcaagcca accggauuaa aggcaaccga acaccauggg aggcuuucgg gaccagcaau     2040

auaucaggau uugacuacag ugguaucuua acgagagccg aacugaugcc uaaggcaaaa     2100

cgcuauaggu ucgcugagga uggcuaccag cguuggcuua aggacgaugc cggguuuuug     2160

gcgcgggccc ucaacgacac cagacaccug uccaaaauug cuagggaaua uaugagccua     2220

aucugcccca auacacgcgu cauuccagga cagaugacug caaugcugcg aagaaacuuc     2280

ggccucaaug augugcuggg ucuuaacggg gagaagaauc ggaacgacca uaggcaccau     2340

gccguugaug cuugugugau cgccguaacc gaccagggcu ugcugcaacg cuuugcagcg     2400

gccucugcuu cagcccguga aagacaguua aaucggcucg ucgagaacau gccccugccu     2460

ugggagaguu acagggaaca cgugcgccga gcaauagaug gaaucugggu uagccauaga     2520

ccagaccacu cccacgaggg cgcuaugcau aaugauacag ccuaugggcu ucggggaaac     2580

gguagggugu cuuuccacaa gauggucgaa ggcaaacgcg aguacauuga agacaaucug     2640

aaggugaucg agauuacuga uaccaaagca aucgacagac auggguugcu cccgacagga     2700

aagcccaggc cuuauaaagg cuacaagggu gacucgaacu acugcauaga gauuguacgg     2760

aaugaaaagg ggcguuggga gggcgaaauc auuagcaccu ucgaugccua ucagcuggug     2820

cgcaaauacg gaaucguuca acuacgauau ccaguccugu ccgugucagg caagccccuc     2880

gugaugagac ugauaaggaa cgacuuuguc cggcuugagc acgaagggau cacucgcacg     2940

uugagaguuu guaaaaugag ggaggaugga cagguggcuc uggcggacgu gacagaggcc     3000

aacguagaug cacggacccg aacuaaggaa auuugcuacg ucuucaaaac cggcgguagu     3060

uuacagaagg cuaaggccag acgcaucaca auuucuccua ucggggagcu cagggaccca     3120

ggauuuaaag aauga                                                      3135


<210>  2622
<211>  3138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2622
augcagaagg agaaaccccu ggccuacaga cucgcucuug accugggcag caccuccuug       60

gggugggcag uguuaaggcu gucugccccu ggagaaccau cagcggucau ccgggcuggu      120

agucgcauuu ucagcgaugg ccgaaacccg aaggacgggu ccucucuagc cguuacacgu      180

agagcagcca gggcuaugcg gcgcagaagg gaucggcucc ugaaacgcaa ggcacgaaug      240

cuugccgcuc uggagagaca cggauuuuuc ccccaagacg aagccgcgag gaaagcauug      300

gagaagcuca auccuuaugg ccugcgggcc cguggucugg aaucggcucu caagccagag      360

gaguuugggc gcgccuuauu ccaucugaac cagagaaggg gauuucaguc aaaucggaaa      420

acugaucgaa aggacagcga uaguggcgca cuuaaacagg cuauaggcac guugcgcgcc      480

caaaugcacg aauccgggug cagaaccgug ggagaguggu ucuggaaggu aaggauggaa      540

cggaaacccg agggugugaa gggccagggg guccgcgcga gauacaggga gacaccuuuu      600

acuaccgacg aaggaaagaa acguaucgau aagcgguaug accuguacgu ggaucgagcc      660

augguugcac aggaguucga cgcucuaugg gccgcucagg ccacauuugc accagcccuc      720

uucaacgaag cugcgagagc agagcugagg gauauucuuc ugcaucaacg caaauugcgg      780

cccgugcagc cuggcagaug uacccucguc ccagaagaac cgagggcccu guuagcucug      840

cccucuacuc agcgcuuucg aaucuaucag gaggugaauc accuccgggu acuucaugac      900

gaacugcgug aggaagccuu gacacuagca caaagagacc agauuguugc ccugcucgag      960

aggcaggcua aggugaccuu cacggccaug cgcaaagcac uggguuuuag cgcgcuuuuc     1020

aaccuggaag augcuaagcg ggacggguug aaaggaaaug ccacuaccgc agcccucggc     1080

aagaaggauc uguucggccc uuuaugggcu ggguuugaca cagcccugca agaugagauc     1140

gucacucagc uaaugaccga ggaauccgag ccaagacuca uagcauggcu gcaggaacac     1200

acaggagugg acgaggcuag ggcccaggcg aucgccaacg cuggucuucc cgaaggcuac     1260

gggucauugg gacgaaccgc acuggcccgc auugugccug agcuccaacg ggaugucauc     1320

acguaugaca aagcuguuca ggccgcaggc uucgcgcacc auagugaucu ggguuuuagc     1380

uucgagcacg acgccgauga aguggagaga auuggggaaa ggacuaucgc uucuacaggc     1440

gagauaaagc caguauuugc cuucaaacag cuucccuacu acggacgugc acugcagcgc     1500

cauguggcuu uugccaagga gaauccgcgg aacgacgaag agagauaugg caaaauugca     1560

aauccuaccg uccacaucgg guugaaccaa gugaggguug uggucaaugc ccucaugugc     1620

cgauacggac ggcccacuga agugguaauu gagcuggcuc gcgacuuaaa gcaguccaga     1680

gaacagaagc uggaggcgca gcaaaggcag gccgauaacc agaaacggaa ugcacgaauc     1740

agacgcucaa ucgcugaggu ucuagguauu agcgaagaga gggugcguac acucgacaua     1800

cagaagugga uccuguggga ggagcuuagu cacgaugccg ccgaccggag auguccauau     1860

ucuggcgucc aaauuuccgc uagcauguug cugggggaaa ggguggagau cgagcauaua     1920

cucccuuucu cgaugacccu ggaugacucu uuaaacaacc gcacggucgc acugcgagaa     1980

gccaauagaa uuaaacggaa caggaccccc ugggaugcgc gcacagacuu ugaggcucac     2040

ggaugguccu acgauggcau ccuucagcgu gccgaaagaa ugccacagcg gaagaggcau     2100

cgcuucgcac ccgacgguua ugagcgaugg uugggggaag auaaagacuu ucucgccaga     2160

gcucugaaug auacucggua ccuaucaagg guggcagccg aguaucugcg ccucguuugc     2220

ccuggcagug cgaccagagu gaucccagga caaaugacag cucugcuuag gggcaaguuc     2280

ggguugaacg acguacuggg auuagacggu gagaagaauc ggaacgauca ccgucaucac     2340

gccgucgacg caugugugau uggcguuacu gaucagcgca ugaugcagcg auuugcccag     2400

gcuaaugccc aagcaagaga agacgggcuc accaggcugg uggaggauau gccgcuuccc     2460

uggccuacau accgggcuca cgucgaacgc gccgugagac auaucugggu aagccacaaa     2520

ccagaccaug gauucgaggg cgcgaugaug gaggaaaccu ccuacgguau ucugagggau     2580

gggacuauaa agcagcggcg aaaagccgac ggcucugcag gaagagagau cagccgcuug     2640

auuaggauca cggaacccuc acagccucag cggcacggcg uggaugcuga ggggcgucca     2700

cuccccuaua agggauacgu uggcgguagu aacuauugcc uggaaaucac agucaaugac     2760

aaagggaagu gggagggaca agugauuucc accuucgaug ccuacagaau agugagggag     2820

ggcggucuac gccagcugcg gcacagcacu cagggccaga acgggcaagc ucucgcaaug     2880

cgacugguca ucggagacuc uguuagacuu gaaguggaug agcaggaaag ggugaugcgc     2940

auuguaaaga ucaauggcaa caaugggcag gucuuuaugg ccccggugca ugaggccaac     3000

guugacgcuc ggaauagcga caaaaacgaa gcguucgcau auaccaguaa gauugccgga     3060

ucauuucaga aaacaaaggc uagacaagug accaucuccc cuauaggcga guugcgugau     3120

ccagguuuca aaggguga                                                   3138


<210>  2623
<211>  3138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2623
augaagccca gaaaccugaa uuacauccuc ggccuugacc uggggauugc cagcguggga       60

ugguccgucg uugagauaga ugaaaacgag uauccuauca gguugauuga cgugggugua      120

cggaccuucg aacgcgcuga ggugccaaaa acaggcgaau cuuuagcacu ggcccgacgu      180

cuagcgagau caacuaggcg gcucauccgc agaagggcuu uucggcugcu uaaggccaaa      240

cgccuguuga agcaccauca gauugucaau gcagaggagc ucacgcaacu gccgaaccag      300

ugcugggaac ugcgagugaa agggcucgau aguuuacuga gcaauaccga gugggccgcu      360

guucuuuugc aucugcuaaa gcacagagga uaccucuccc agaggaagaa cgaagcacag      420

aaugccgaca aagagcuggg caagcugcgg gaggguaugg auaacaauuc uaaacuguug      480

cucgaaaaca auuaucguac acccgcugac aucgccguga agaaauucgc ggucgaggaa      540

gggcacaugc gcaaccaaag aggagcauac acucauaccu uuaauaggcu ggauauauua      600

gccgagaugc agcuucucuu caagacacag cgggaacuug gcucgaccua ugcuaacggc      660

gagcuggagc aggccuuuug ugaauugcua cuguggcaaa agcaggcacu caaucgauca      720

cagaugcuga gccuuguggg gaaaugcacu uucgagaagg aagagaaacg cgcugccaag      780

gcgaguuacu ccgccgaaag auuuguagca auccagaaac ugcaaaacuu gaggauucuc      840

gagaacggag aggaacgggg ucuguucgac ucugaguuua gcuuacugcu agaaaaugcu      900

uauaaccuca aguccggccu gacauacaaa cagguucgca agauccuuuc auugagugag      960

aaugccauuu ucaaggggcu gccuuaccuc agcgaugacc uggaaaaacc agagaagacc     1020

caguuucuug cuuucaaauu uuaucaccaa cuggccgaua ucuugaagaa caauggauuc     1080

ucugacgagu ggcagaaacu cucccaggaa cccacgcugu uagauaagcu gggcacugag     1140

cuaucacucu guaaggaaga gaacgaguuc auagcacagu uuaaugguga acugccugag     1200

gccaugcuua gcaccuuguu caaccacaca aauuuugaca aauucauuca uaucagucug     1260

aaggcucuca acaauauucu gccauuaaug gaacaaggga acgacuacac uaaagcgugg     1320

agaaaggugu aucccgagcc uaccaaaaag gaugaaaaga cacugccacc gauccccgca     1380

gacgagauca ggaauccugu cgugcuucgu ucuuuguccc aggcccggaa agugauuaac     1440

gcugucauac gacucuacgg aagcccagcc agaauccaca uugagaccgc aagggaacug     1500

ggcaagucgu augaugaccg ccagaaaauc aagaaacagc aagauaaaaa uucugacgag     1560

cgggaucagg ccguuaagaa guuucuagaa gagugcccca acuucgcuaa caaggugaaa     1620

ggcaaggaca uacugaaaau uagacucuac aucaaucagg augggaagug ucuguauucc     1680

ggaaaaccuc uugacccaca uagguugcug gaaaucgguu acguagagau ugaucacgcc     1740

uuacccuuuu cacgcacgug ggacgacagu cagaacaaua aggugcucgu ccuggcaaac     1800

gagaaucaaa acaagggcaa ucagacuccg uucgaauggc uugggaaaga ugagcaccag     1860

ugggcgcugu uuguggcucg aguuaacgga ugccgguucc cuuacgccaa gaaacagcgu     1920

aucuugacaa agaaacucga cgaacaaggc uuucugaaga gaaaucuaaa cgauaccagg     1980

uaugugagcg cauaccugau gaagcauauu aaagagaauc uccaccuggu cgguaagggg     2040

uccgacaaag uguucgccuc uaacggccag guaacuaauu uucuucgccg guguugggga     2100

uuggaaaaga aaagagagga gggcgauagg caucacgcuc uggacgccau aguuguggca     2160

ugcagcacag cuucaaugcg acagaagauc accuuauuca agaaauauca acgcuggaac     2220

cucaagacgg ggaaacauau ugaucaggaa accggagaga ucaucccccu gcacuuccca     2280

gccccuuggg acuuuuuccg gcaggaaguc augauuagaa uauuuaguga gaugccccag     2340

gaggaucuua ucaugcaacu gccagacagg ccccaggcga aucacgaguu cgugcagccu     2400

cuauuugucu cccgugcccc aagccgcaag augucugguc agggccauga agcuaaacuc     2460

cggucagcaa gaaugcugga agccacaggg aagaguauua agaaagaguu cuugacugau     2520

cugaccauca agaacuuaga acacauggug aacaaagaga gggagccgga acucuaccaa     2580

gcucugguug agcauuuuaa gaaauaugga gacaagccca aagaaaaguu cuuuaagaaa     2640

ggcggugugg agguaaagag cauucgaauc uccaaaacac agaauaaguc ugucaaccuu     2700

gggaauaaaa cuauagccga uaacggcgac aucgugcgga ccgaccuguu cuugaagaau     2760

aagaaauacu auuuuguuaa cauuuacgca uggcaagugu cgaagggaau ccucccuaaa     2820

gaaacaacca cuggcaauau ucuggauaac agcuacgagu uccaguuuuc ccuauucaag     2880

aaugaccugc uugagauccc acaccccaaa aacgaaaaug auucaauacu cgcguauuuc     2940

auucgcccug acgaugagag aagguggauc cugaaguuuc acgacaacgc caagauucca     3000

gauaucuacg ggaaaaagga cgaaacgagu auacgguugu cuauccaggg acagaaauuc     3060

auuaagaaau aucaggucga ugagcugggu aagaauaucc gacccuguag accgacaaag     3120

cgccaaggcg ugagguga                                                   3138


<210>  2624
<211>  3141
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2624
augaagaucu acagcuucga cauuggcgug gccuccauag ggugggcuuu uaucgagaac       60

gaaguccugc aggauugcgg aauuagaauc uucaccaaag cagagaaucc caagaacggu      120

gccucucucg cgcuuccuag gcgggaagcu cgcggcguuc gaaaacgucu ggccagaagg      180

cgggggcgcu ugaauacaau uaagcaguua cuguguaaag aguuuggacu acagcucgaa      240

gacuaucugu caaacgaugg cgagcuucca aaggcauaca cugccaguaa ggcuaauccg      300

cugaaaagcc ccuaugaguu gagaacgcuc gcacuguccc agaagcugga cucuaaagau      360

cucgugaggg uaaucuuaca cauagccaag caucgggguu acgggaacaa acacgcuaag      420

gacucgaagg auaccgaauc aggaaaagug aagaaagcca ucgaguucaa ucgcaagaca      480

cuggcggaaa aaggcuaucg aagcgucggc gaguaccuuu auagagaguu uuuccagcaa      540

aguaggauuu cccagaacaa uggggcaacu gaauuuguga acguucggaa uaagaccgga      600

aacuacgagc auugcguggc ccaggacaug uugaaagaug aacuggagcu aaucuuccgu      660

aagcagaagg aacucggucu gcaccuuucu gacggcuuug agaaacaacu guuggauaag      720

auuuucgagc agcgcccucu caagagcuuu gcugacaaag ucgggaaaug ucaguucauc      780

gccggagcaa agagagcucc aaaggauucc auaucagcca uugaauuugu ggcgcugagu      840

aggaucauua auacauuagc caaccugagc aaaaagucug gcgagaugua cgacaaagca      900

augguacuca ccauccuuga cuccguucug gaaaagggug agaugucaua uuacgcuuug      960

cgggaagcca ucaaucuaga ugagaaaauu cgauucgcug acagccgccu ggauuauagu     1020

aaggagauaa aggaaacuga gaaagugaag uucgucgaau gcaaaaaccu caagucuuuu     1080

aaaaaggugc uggggaacug uuacaagaca cuugagagaa aacauaucga cuccauugcc     1140

gaaaggaucg cagugauaaa ggaugucgag aaacugcaca aggaguugga aagcuauugc     1200

gccaccgagc agcucucgcu gacgucugaa caaauucagg cuuuauccca ccugaauuuc     1260

ucaagucaua ucagccuauc ccucaaagcg cuggagcaga uccuucccuu uaugcgggga     1320

gagcgcgaag caagaucuag cgacgccgau uacuguauug gcaucgacga gucaggcgaa     1380

agucaaugcu ugagguauga ugaguccguu gaaaaggcug ggcugaaagc ccucagcaag     1440

gacacucuga agggacagau ucuuccucca cugaacgagu ucgagcccua cuuggcaaau     1500

ccugugguag cccgugcucu cgccgaauac cggaaagugc ugaacgcauu acugaagcag     1560

uaugguucuc cacacaaaau acauaucgag uacgcgcgag aagcuaagcu aaauaccaca     1620

gagagacaga aauaugaaaa ggagcaaaag gauaacuacg ccgcaaauca ggccgcucac     1680

aaaaaguguc aggagcucgg ccuggaaccg ucaaguacua accuuuugaa acugaagcuc     1740

uggcaggagc aagggggacu gugcuuauau agcggcgcca aaauuaccau cuccuaccug     1800

caggacccca cagcacuuca ggucgaccac aucuauccuu acucuagguc guuugaugac     1860

agcuacauga auaaguguuu ggugcucacc aaggcuaacc aggaaaaagg uaaucgcacg     1920

ccauucgagg ccuuugggac ugauuccgcg aaguggggcg uuauuacaac ccuggcccaa     1980

aaacuacccc auaagaaacg gagaaggaua cugaacacug cuuucaauga caaggaagca     2040

ggauuuaagc uccgcaaccu gaaugauaca ucauauaucg cccgacuugu ggcugacuac     2100

accgagacgu auuuggaguu ccugaguuua gucgaaucug agaacaccac acucggcaaa     2160

gggcagaagg gaagcaaaaa gcacauugcc aucgugaacg gugcacugac uuccaccaug     2220

cgguacuauc uuggcuuugc gucaaaagau cgugacaauc aucugcacca ugccuuggau     2280

gcuguaauua ucggguucau gaacgacagc guuauaaagg ccuuuucuga uuucaaaaag     2340

acacaggaaa cuuccaaggc agcuuacuau gcccacaccc ucaguaaaca ggaguacaag     2400

aaacaaagag cauucaucca gcugccuagc ggagagaauu uuagggccaa cgugcuagaa     2460

aaggcugagu cugucuucgu guccaaacca ccccgcaagc gggucagagg cgcgcugcac     2520

gaagagucau uuuacaguca gaaugacaca aagcuccuga aaagcuaugg uggggccgaa     2580

ggcauugaga aggcacuuca auugggaaaa aucaggcaga uuggcaccaa gaucgugucg     2640

aacggggcua ugguucgagu ggauauauuc aaacaucgca aguccggaaa guuuuacggu     2700

guaccggucu auacuaugga cuucgcccug ggcauuuuac cuaauaaagc cgugguucag     2760

gggaaggaca aaaacgaugu gaucaaggac uggcucgaga uggauucuag cuacgaauuu     2820

auuuucucac uguauaaaga cgaucuucug cuaguccaga agaaggagau ggcugaaccc     2880

gagcucugcu acuuugugag uuucgacacg uccggagcau cuaucaaagu agccaagcac     2940

gauaauaacu uuagcacacu gaccgaaaau caaaaauugc uguucuccaa cgcgacuaag     3000

gaggaggugg uuggcaaauc aauagguauc cagaauuuaa aggucuuuga aaaguggcaa     3060

gugagcgugc ucggggaggu caaacggacc cagaguuauc caagagaaaa cauuucucug     3120

gcuuccaagg ccaaaaagug a                                               3141


<210>  2625
<211>  3144
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2625
augaagcugc ucagauacau ccuuggccug gacuugggga uugccagcgu gggaugggcu       60

auagucgaga ucgaugaaaa cgacuucccc auuagguuaa ucgauauugg uguucggacc      120

uuugacaaag cagagguguc caagacaggc gaaucucugg cccuagcgcg ccgacucgcu      180

cguucaacua gaaggcugau ccggcgcaga gccuucaggc uucugaaagg gaagcgguug      240

cucaaacagc acaauguaau aaacaaggag gcacugacga cccugccuac agauugcugg      300

gcccuccgcg ugaagggauu agacgaacga cugaguccaa cugaguggag cgcuguccuu      360

uugcaucugc uaaaacacag aggcuaucuc ucccaaagga agaaugaggc aaaaucugcc      420

gauaaggaaa ugggugcucu gcuuucgggg guggccucaa accaucagcu guugguugag      480

aaucaguuuc ggaccccggc ggaaaucgca gugaaaaagu ucgcccucga ggagggacac      540

auucguaacc agcugggcgc uuacacacau accuuuaauc gcuuagaccu gcucgccgaa      600

augaaacuuc uguuccaaac ucagagagag uugggcaacc cccacacaag cgggcagcua      660

gaacaggagu uuugugaacu gcucaugugg caaaagccug cacugagugg agaugcuauc      720

cuuaagaugc uggguaaaug caccuucgag acgaaugagu auaaggccgc gaaacacucc      780

uacacugccg aaagguuugu cugguugacc aagcucaaca aucugcggau uuuagagcau      840

ggcucugaac gagugcugaa cacagacgag cgcagccuac uccugaauca gccauaugaa      900

aaagcaaaac uuacuuacac ccagguaaga aaguugcugg ggcucuccga ggaggcuagg      960

uucaacggag uucgguaugg caaggaaaag gccgaggaug cuacacugau cgaacuuaaa     1020

gccuaccacg caauacgcaa ggccguggag aaagcugaca agucagcgug ggaaacccug     1080

aagaaucaga cggcauugcu cgaugagauu gguacuacau uuaguaucua caaaaccgac     1140

gacgauauua cugagcaacu gaacgggaag uuaagcgaca caguccugaa cgcccuacuc     1200

gaaaaucuga acuucgauaa auuuauccag cuuucuuuga agucccugua uaauaucgug     1260

ccccucaugg agcagggacu gagauacgac gaggcuuguc aacagauuua uggcgaucau     1320

uucggcaaaa agucagggga gauaaacagg uuacugccuc caauccccgc cgacgagauu     1380

cguaauccug ugguccuucg gagcuugacc caggcacgaa agguuaucaa cgccgugaua     1440

agacucuacg gaaguccagc uaggauucac aucgaaacgg cccgcgagcu ggguaaaucu     1500

uauaaggauc ggaaagaaau cgagaagaga caggaacaaa auagggcaga gcgcgagaaa     1560

gcgaccgcuc aguucaagac acuauuuccg acuuucauug gcgaacccaa ggggaaagac     1620

auccugauga ugcgacucua cgagcagcag aacggacaau gccuguauuc cggcaagccu     1680

cuuaauuuga accggcuguu agaaaaaggu uacguagaga uugaucacag ccucccauuu     1740

ucgcguaccu gggacgauuc ucagaauaac aaggugcugg uccuugccgg ggaaaaucag     1800

aacaaaggca aucagacacc cuucgagugg cugggaaagg acgagcauca augggcauug     1860

uuuguggcca gaguuaacgg cuguagguuc ccuuacgcua aaaagcagcg cauacucacu     1920

cagaagcugg acgaacaggg guuuaucgag cggaaccuaa augauaccag auauauugcc     1980

agguuccugg ugaacuuugu ccgagaaaau cuccaccuga caggaaaagg uaagaaccgc     2040

guguucgcau ccaauggcca aaucaccgcu uuucuucggg ggagaugggg auugucaaaa     2100

guaagggagg aaagugaccg ucaucacgcc cuggaugcgg uuguggucgc cugcagcacu     2160

guggcuaugc agcagaagau cacgcgcuuc gucaaauacc aagagggcaa cuuacagaca     2220

gcagagcgga uugacagaga aaccggugag auaaucccac uccauuuucc cgauccgugg     2280

cacuucuuca ggcaggaagu gaugauucga aucuuuuccg agaauccuca ggaggaccug     2340

auuauccaac uucccgaucg gccagaagcc aaccacgagu ucguucagcc ucuguuugug     2400

ucucgcaugc ccacuagaaa gaugagcggg cagggccaua uggaaaccau aaagucagcu     2460

aaaaggcuag acgagggaau caguguaucc aaggucccac ucacacagcu gaaauugaag     2520

gaucuggaca auauggugaa ccgggaacga gagaaagccu uauaugaggc acucaaggcg     2580

agacugaaug ccuucaacga ugaccccacc aaggcuuuug ccgaaccuuu cuacaaaaag     2640

ggcgggcaac agguuaaagc agugcgcguc gaacaggugc agaagucugg aguagugguu     2700

aggaauggua acggcgucgc ugauaaugcc agcauggugc guguggacgu cuuuauuaaa     2760

gggggaaagu auuuccuugu uccaaucuac acuuggcaag uggcaaaggg cauucugccg     2820

gaccgggccg ugguagcugc gaaagaugaa ucagaguggg aauugaugga caagacagcc     2880

caguuucucu ucaguauguu ucccaacgau cugauccagg ucaaaacgaa gaaagagacc     2940

uucuuugguu auuacaacgg gcuagacaga gcaacuggcg cuauaaaucu gaaggagccu     3000

gauagcaaca uuaauaaagg aaaguccggc cuucacaggu cuaucggggu gaagacagcc     3060

cucucgguug aaaaauacca aguggacgag cugggaaaga acauucgccc augucgaccc     3120

agcaaaagac aggccguccg guga                                            3144


<210>  2626
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2626
augaagagac ugggccucga caucgggacc aacagcauug gauggugccu uauagaggau       60

gacggugaug gcaaagggag gaucauugac aucggagccc ggauuuucuc cgauggccgc      120

gaccccaagu cuggugcuuc acuggcagug gaucgacgug ccgcgagagc uaugaggcgg      180

cgcagagaca gguacuuggg gcggcgcaca gccuuuuuaa aagcacugaa gcgauucgga      240

cuaaugccug ccgaugaaag ugcugcaaaa cuccuggccg acagagaucc auaugagcuu      300

agggcucggg cccuggacga acguuuggag ccgcacgaaa ucggccgcgc gcucuuucau      360

cugaaucaga gaaggggcuu caagagcaac cggaaggcag agcgaaaaac uaaggaugcc      420

gaggacggga aaauagcuaa uggagccaag gcacuggacc aagcuauggc cgaauccggu      480

gcggagacgc ucggccaguu uuuaucuucg cgcgauacca aaagagucag gaugggguca      540

gacgcccagg gauacgauuu cuauccccag cggcgccacg uugaauacga guuugacaca      600

aucuggagca gacaacagca gcaucacccu gcacugcuua cugaggaagc uaggaagaag      660

uugcaugaua uucuguucuu ucagcgucca cuaaaagagc aagaaguggg cguauguacc      720

uucgugcaca acgagugccg gcuccccaag gcccacccuc uguuucagga acgaagacuu      780

uaugaggagg ucaaucagcu ggaaaucaca accccaggug cucccaacag gaaauugacu      840

auggacgagc gcgaugcccu cauucuggaa uuacggagua gaaaggagag guccuucgac      900

ucucuggcaa aacgccucaa acuugccccu gggcagagcu uuaauaaagc auccgaauca      960

cgaacacggc ugcguggaga ugagguguac gcgguuaugg cacauaagaa gagauucggc     1020

gacagguggc cacacuugga ucgcgaccgg caaugggcca ucauaaaaag aauuaucgag     1080

gaagaggacc cggauaagcu acaugcuuuc cugaccggcg aaugggggcu cacggacgag     1140

caggcccugg caacugccag ggcuccccuu ccugaacugu auggacgauu ggguccaacc     1200

gccacagcac gcauucucga ggagcugaaa aaggauguga ucacuuacga cgaagcgguc     1260

cggagagcug gcuggcacca cagugaugac aggaccgggg agaucuuacc ccagcugccu     1320

uacuauggag aacuacucac acgugauauu ccacccggca cccaagaccc gaacgauccu     1380

cccgagcagc gcugggguaa aauaacgaau ccaacugugc auaucgggcu gcggcagcuu     1440

gagaaguugg uaaacgccau uaucaagguu cacggcagac cugaccagau aguggucgaa     1500

cuggcaaggg agcucaaacu gaaugaaaag gauaaagagg aacauaaccg acggauucgc     1560

agagacacag ccgcugccca acuuaggagc gcaaagcugc agucugaggg aauccccgau     1620

accggcgcua aucggauguu gcuccgaauc ugggaggaac ugaacccagg ggagguguua     1680

gacagacgcu gucccuacug cggagaaccu guggguauga ggaugcuguu ugccggcgag     1740

gcggacauug aucacaucau uccauauucc cguucacuag acgauagcgc cgggaauaaa     1800

gucguugcuc aucggaguug uaacagagca aagggaaaca ggacuccgua cgaaaagugg     1860

gugcacgacc ccgaucgcug ggacguaaua ucuacacagg uggcccgacu ccacaaaucc     1920

aagcagugga gauucggccc ugaugcuaug gcccgggucg agaaagacgg uggguuuauc     1980

gcaaggcaac ugaccgauac gcaguaucuu agccgcuugg cgggccguua ccugucgucu     2040

cucuauccaa ccaaggacga uggauccgug uacguuauuc ccggcagaau gacagccaug     2100

cugcggaggc gcuggggguu aaauucacug cuuccugacc auaacuucgu ggauaaugag     2160

cacaguaacg cuccaaagaa ucgauuggac cauagacacc acgccaucga cgcagcuguc     2220

guggccguaa cuacccgggc acucaugcag gaaaucgcca ggacagcugg acgcgcggag     2280

gauaaggacc uggauagacu auuugaagac cugccccagc cgugggaggg uuucagggag     2340

gaacucggcg agaagcuggu ucgggugacu gucagccaua aaccugauca cgggcguaag     2400

ggacuuccac ccaaaaaccg cgacgugacc gccggccgau ugcauaauga uacagcauac     2460

ggucugaccg gggacgucgc ugccgauggc aagacuccua uugugguuca cagagugccc     2520

uuaauguccc ucaaaccagc cgacauaucu gauccugaca ggauccccga cccagcucug     2580

cggagcgcac uucaucgcgc cacggaugga cugucaggca aggcguauga cgcugccuug     2640

gcaggguuug ccaaaacaca cccgcaauuc gcuggaauua gaaggguacg gguccgagaa     2700

ccucucaccg ugauccccau ugcagauaga gacggccgcc cauuuaaggc cuacaagggu     2760

gaugcgaaca guaggguuga caucuggcgg augcccgaug ggaaauggcu guccgacgug     2820

gucacuaugu augaggcuca ccguagagau cauagcgaca ggcgcccuca cccagccgca     2880

aagaaacuaa ugucucugcg gcagaaugau augauagccg uggaacgaga cggagcuccc     2940

agacagauca ugguagugaa gcagauuugg ccuacccaaa ucaaccucgu ugagcauaaa     3000

gagacaggca aucugaagga uaggcacuca aacgaaaccg acccguuccg cuuuuucgcc     3060

ccaacugcug guggccuuaa gaaaaugaag gcucggcagg ucagaauuga ccccuugggg     3120

cguaucuuug auccuggacc aagguga                                         3147


<210>  2627
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2627
auggccaaca ucgcugagaa ucugguguuc ggcauugacc ucgggauagg aagcugcggu       60

ugggcagucc uuaaccagaa ggccaaagau gaaaauaucg uugcgcuggg cuccuggugu      120

uuugacgugc ccgagaccga uaaggaaagg auuccuacaa accaaaucag acgggcuaau      180

cgcuuguuac gacguguaau uagaaggcgg cgcaacagaa uggccgagau caggcggcug      240

uucgcaucua aagggaugcu agacacugcc gauccagacg cucucgcaaa gauaucagcc      300

gauccguggg aaaugcgcgc uaaaggacug gacuaccagc uuacgcccca ggaguuugcc      360

guggcgcugg gccacaucgc aaagcgaaga gguuucaaga gugccgcuaa aagggucagc      420

accaaugccc cuuccgagga uucuaagaug uuggcagcuc ucgagaaaac aaaggagcag      480

ucggcccaau aucggacuau uggggaaaug uuugcgcgug acccagccua cgcagaucgc      540

agaaggaacc gggacggaug cuucgaucga accaugucac gcgaggaccu guuuaaagag      600

acacagaucc uguucgaaag ccagagaagg cucggcaauu uuagugcuac cuccgagacu      660

gaacaggccu ucauugacau cgcuuuucgg caaaagccca ugcaggauuc ugagcgcuua      720

guggaaacau gucuguucga gccugacgag aagagagcca gcagguuugc accauccuuc      780

gaacguuucc ggcuuuugug ccgacugguu aaccuaagag ugaccgaugg ccucgaggac      840

aggccccuga cgccugaaga acuucgccug gccugugaag augcugggaa aacucggaag      900

uugucaguca aaaaggugag aagucucaua ggacugaggg acgaucagcg cuuuaccaca      960

auuccaccgg accaggagca acaugauauc gcggcacgaa cuggugacgc cuuaaccggc     1020

acagcuaccu uccgggccau ucugggggag agcuuuuggc gucagcuccu uccccacccu     1080

gaacagcugg auagagcagc cuggaucauc ucuuuccaug aguugacgga cacuauuaua     1140

uccaaacuag cuacaaucgg acugccauca gaaguaaugg agcucauuag gaaggcccug     1200

gaagacggca aauuuagcaa guucaagggu gcagcgaguc uuucugauaa agcugcccgc     1260

aagcugaucc ccuuuuugca agaggggcuc accuaugaca aagcaugcca gaaggccgga     1320

uacaaucacg cugccuccaa auucacucau uaugauagca uagacacaaa ggcaaaguuu     1380

aacucgcuga uggaugacgu ucgggauucu auuaccaauc cuguggcuag aaaagccauc     1440

uccgagggca ugaagcaggu cugggcgaug aggaaccagu ggggcuuacc aggggccauc     1500

uguauugaac uggcucgaga cgugggaaau ucagcagaga aacgccggga aaucgagagu     1560

gguauuaaga aaaacacggc ccaaagagag agggaacguc aggaggcuaa ggaacaccua     1620

aaccagagcg auauauccuc ugagacccuc cugcgcuacc ggcuuuggaa ggaacagggc     1680

gggagaugcu guuauacaga ccaacccauc acuccgagcc aguugauugc cucagauaau     1740

agugugcagg ucgaccacau ccugccuugg uccagguucg gagaugacag cuaccucaac     1800

aaagcacugu gcaccgcgaa ggccaaucag gagaaaaaga accgaacacc cugggagugg     1860

auccauaauc aaaaaggcgu ugaggcuugg aaccacuuua cugccgcagu ggaaucucgg     1920

aaggaaaccc gcggucuuaa gaaaagaaau uuccuguuga agucaaguaa agagacagaa     1980

gagagguuuc ggagccgaaa ccucaaugac accagauacg cugcccgcgu aauggcagag     2040

gccgugaggc uguuauaucc auccgggaac cgugcugaaa agggcggagu caaacgggug     2100

uucacuagac cuggcgcgcu gacggccgca cuaaggcaug cuuggggagu ugagucucuc     2160

aagaaaauua augggaagcg ccugaacgau gaccgacacc acgcccuuga ugccgcugug     2220

gucgcagccg ugucggaaaa ggagauacag aaauugacaa agagcuucca gaucugugaa     2280

cagaaagguc uggcgagacc caugcgggac guaccaaugc ccugggaggg cuuuaggcau     2340

caacucgaga ccgcuuacgc cgauauucgc guugcacguc cugaaagacg gagggcccgc     2400

gggaagggac acgacgcuac uauccgacag cugagagagg aaaaagaugg cccagugguc     2460

uaugagcgga gggcaauuuc cgacuuaaag cugaccgauc uugagcgcau caaggacccg     2520

gaaagaaauu cagccauaau caguucuuug aggacaugga uugagaaagg uaagcccgcg     2580

gauuacccuc cacagagccc caaaggggac gaaauccgga agauucgugu gaugucccgc     2640

aacaaaccug cugucucagu gcgaggcgga accgccgaua gaggcgagau gacuaggguu     2700

gacguguuca caaagauggg gaagaaagga aagccagaau gguuucucgu acccaucuau     2760

cggcaucaag ucauagaccg cauucagugg ccgcagccuc caaaucaggc agccgcuaac     2820

gguaaaagcg agaagaacug gcccacgaug ggccuggagc acgaguucag auuuucucua     2880

uacccuaggu ccuaugugca aaucguuaaa cgggaugggg aaauuaucga aggauacuuc     2940

gccggccugg accgaaccgu ggcagcuuuu agucuccugu cuagcuccga ucccacuuca     3000

auacagagag guaucggggc caagacacuu gagaguauuc gcaaguacaa ugucaacagg     3060

uucggcgaca ucagcauggu ggaacaggag acccggacau ggcauggaga uguaugcauu     3120

ucgccagacc agccugauuc cgcguga                                         3147


<210>  2628
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2628
augguggaga agauccuggg cauugaccuc gggauaagcu cccuuggaug ggccgucguu       60

gaauacgaua aagacaacga ugagaauaac aagaucauug acugcggugu gagacuguuc      120

accgcugcag aaacacccaa agagaaggaa ucuccuaaua aagccaggcg ggaugcgcgc      180

ggcuugcgac guguaaucaa gagaaggcgg gugcgcauga acacuauuaa gaauuuacug      240

aucacguaua aacuaauaga caagacccuc cuggaugagg agauggggau guuucacuca      300

cagaguaaca gagucgacgu guggaaacuu aggcaugaug cucuguacca cuugcucagc      360

ggagacgaac uggcccgggu ucugauccau auugcaaagc accgcggcua uaaauuccuc      420

ggugaugacg aguccgauga agagucuggg aaggugaaga aagccggagc ugaguuaaag      480

aaaaaguuuc uggaagcagg cugucaaucg gucggcgagu ggcuuuggaa agaacgaggg      540

uugcagggaa agaaaagaaa uaagucaggu gacuacgaga ucagcauucc aagggacuuc      600

cugguggaag agauccagcg gauauuugag acacagcaaa aguucggcag uacuuuugcc      660

accuccgaac uacagaaggc uuauacagau auugccuucu acguaaagcc gaugcagucu      720

aucgaggaca ugguugggua uugcaccuuu uaccccaaaa uuaagagcaa aaaccaggau      780

ggagaaaagc gugcgccuaa ggcaucccca ucagccgagc aauucgugau ccucaguaaa      840

aucuuuagca cuauugucau agacgaaaau aagcaggaga aaaagcugau cgagcuuaaa      900

ucuauugaac agcugaucca gauagcucgc uccaaggaga cauugaagua caaacaacuc      960

agaaaggaac ugaacuuagc caaagauauu ucauucaaga gcaucaguga cgaggagaaa     1020

accuggauca aucugguggg caacgcaaag uuuaagaaaa uucucggucu uaauuaugaa     1080

acguuccuga agaacacuga gaucucugau gaaauugcua aaauauugac cuacgacaag     1140

acauucgagc agaaagaaac uaagcugaaa aaucuacucg ugaacaucga uuggauugac     1200

aacaaucaua ucgccgagcu ggcgaaacuu uccuuuagcc aguucaacca acugucguug     1260

aaggccauca aaauuauauc uaagaucaug auugaggggu augcaaggua cgaugaagcu     1320

guccaguaug ccuuugagaa uaaccuccug cccaaaccuu cccacgaaaa gucaaucuua     1380

cugccacccc uaaaagagac caauauugcu auccucaacc cuacaguuau acgggccuuc     1440

gcacaguuuc gacagguggc caaugcucug guaaguaagu acggcagcuu cgacaaggug     1500

cacuuugaac uugcgcgcga ggucaacacc aaagaggaca gaaagaggug ggaaaaagau     1560

cgggacaaga augagaaaau gcaucgccaa aucacggaaa aguuggugga ggaaggaguu     1620

aagccauccu auaaaaacau ucugaagucu aaacucagaa gcgagcagaa ggauacuugu     1680

ccguacugcc agaaaaaucu gcacuacccc augaucuucg aggacggcua ugcagaaauu     1740

gaucauaucc uuccucuguc acagagucaa gacgauuccu acgugaacaa ggucuuggug     1800

cacagcgccu guaaucagaa caagaaaaau aggacaccau uugagugguu ccaggacgaa     1860

aagaaagauu gggacaccuu uaagucuuau auacucaugg agucaacucu gggggagaaa     1920

aagcguaacu acuuaauuaa agaaaacuuc agugaucccc agagccggaa ggaguuuauc     1980

ucccgaaauc ugaacgacac aagauauaug ucuaaggcua uuaaaaccua cugcgaaaau     2040

cacuggaagc uaucgcauga ugacgacaaa cucaggaucc aaguacgcag cggaaagcug     2100

acguccaccc uucggcacca gugggguuug gauaacaaaa auagagagac acauacucac     2160

caugccaugg acgcaauaau gaucgccuuc ucaacccagg gcaugguuaa gaagcugagu     2220

gauuauuucg cuaaaaagga agccaaagug gagaaggaca aaccuguccu cauuacacca     2280

aucaagcagu uuaaggaggc aguggaacaa gcgacuaccc uggagaggca ggaaucuauu     2340

cagacaaaag cuggggauac caucacuuua aaccgccugc uuauaagccg acccccgcgg     2400

gccuccguca cgggagcagc ccacgagcaa acagcuaagc cuuacccccg uauuaaacca     2460

aucaagaaua aauacaagag aaggcgcauc ccuauugacg aagauaaguu cgaguuguuu     2520

cggaacgaca aaguggccuc aggcaaugau aagaacuucu auaauagcuc uaccaucccc     2580

agaguugaca uuuacaaaaa ggaugacaaa uaucacgugg uaccaauaua ccucuccgau     2640

augacuaagg cagagguccc caacaagagu cuggguacca auccugaagg gauggacgag     2700

aaauauuuuu guuucagcgu guuuaagaac gaccuaaucg aacuggagac aaaagcuacc     2760

ccaaagaaac cgucuaagaa acuccugggc uacuucaagc agcuuaaugg agccaacuuu     2820

auuuugaacu ccauccauaa uggcauuauc gauggguucg uuugcucacc cauaacucug     2880

uuuaagcagc agaaagacau guguaagaaa ugcuuaccug aagauagggc gaucggaaac     2940

uguagucaag agacacucga guucugggaa gccgagaaua uuaaggugcc caaaaaggac     3000

uuugaaugcg aucaggguau caaguucgcu auugcagucc gaaaauauac gaucgaccca     3060

cugggcuacu accacgaggu gaagggggaa aaacuucugg gaaccauacc ucagggcgcc     3120

aagaaacauc caaagcgcca gaaguga                                         3147


<210>  2629
<211>  3150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2629
augaucaacg agagacugac cuucggcauu gaccucggga uaggaagcug cgguugggcc       60

gugcuuaggc acgcugaugc agccggcgaa cccgggguca ucgagggacu gggcuccugg      120

uguuuugacg uuccugaaac aucuaaggag cggauuccaa cuaaucaggu gcgccgauca      180

aaccguuugu uaagaagggu aauccggcgc agaaggaauc ggauggcgga aauucgccga      240

cuguuccaug cucaaggucu acucccgagu gccgaugagg acgcacugaa aagacccagg      300

cuugauccuu gggagcugcg ggcccguggg uuggaccagu gccucagcgc ugaagaguuu      360

gcaguggccc ugggacacau cgcucgccau agaggcuuca gguccucugc caagcggaaa      420

gcggcaaaca cgucgggcga agauucaaag augcugagcg cccucuuagu cacccgagag      480

cgcacagcua gauacaggac ugugggggag auguuugccc gggacccaga auauagugca      540

cgcagaagga aucgugaugg aauauucgac cggaccguug cucgagauga ccugauccac      600

gaggugggua aacuuuuuga agcccagaga gcgaggggcu ccgccuucgc aucucccgau      660

uuggaggcug acuuuaacgc cauugcuuuc cgccaggccg aaaugcaaga cagcgagcgg      720

cuggucggga auuguccuuu ugagagagau gaaaagaggg cagcccgcug ggcuccaucc      780

uucgagcgau uucggcuacu cacacgucug aucaaccuua gaauugcgac cgcagaagga      840

gagaggcgcu ucacucccga agaccuggcc cgggugucag cugaguuggg cgccacagca      900

aagcucccug uaaguagagu uagggcccug aucgguuuac ugccagauga gcgauucacc      960

acgauacgcg uggaagacga ggaucgggac auugucgcua gaacugggga agccgcaccg     1020

ggaaccugga ggcuccguaa aacacuuggc gaggcgcugu gggcugaaau gcaggcccgc     1080

cccgaucagu uggacgcaau cgcccaugug cugagcuuuu ucgaggcuac ugccaagauu     1140

cggucugagc uggauagacu cggccugccu gcagcuguga ucgaagcccu ugacaggggg     1200

cuggaggaug gagcguuugc caaauucaag ggugcuggcc acaucuccgc aaaagccugc     1260

cgaaauuugc ucccacaccu acagcaaggg caucgguacg accaggcuug ucgcuuagcc     1320

ggauaugacc acgcagcguc aacccugagc cauacagccc aggucguuag uaaggcuggc     1380

uuuaacgccc uagugaccga acucggugag ucuauugcaa aucccauagc uagaaaggcc     1440

cugacggagg ggcuuaaaca gaucagggca augugcgccc gguggggcuu gccuggagcu     1500

auucacaucg aacuggcgcg agagguaggc aacuccauag aaaagagacg cgagcucgaa     1560

agccaaaggg agacuacaac caaacgucgg cagacugaga gagaugaaau uagggggcug     1620

cuugcccugg aggacgugga ugacgauaca uugcuccgcu accgacugug gaaggaacag     1680

ggagguaaau guuuauauac cggcaaggac auccacguca gacaacugau cgcaacggau     1740

aauucggugc agguugacca uauucuacca uggucucggu ucggggauga cuccuuuaac     1800

aauaaaaccc ucugccuggc uucagccaac cagaggaagc gcggacagac acccugggag     1860

ugguucaguc uuacuggcac cccggaagcc ugggaggugu uugcuagccg ugucgagaca     1920

uccaaggauu ugagagguuu caaaaagcgg aaccugcucc ugaaaaaugc agacgaagug     1980

gcccaaaggu uucgcucucg aaacuuaaau gauacuagau acgcggcucg gcugcuugcc     2040

gaggcagcca aguuguucua uccugcuggg gacaggcagg aaaaagacgg ccgcagaagg     2100

guacucaccc ggcccggacc acugacagca gcccuacguc acgcgugggg ccuggagagc     2160

cucaagaagg cugaugggaa acgcguuuca gacgaucgac aucacgcccu ggacgcacuu     2220

gccguggcug ccaucaguga aagagagguc cagagguuga cccgguccuu ucaggagugg     2280

gaacaacagg gacuggcacg cccuuuaaga cauguggcuc ccccauggcc cgguuucagg     2340

ggcgaugucc ucgccgcgua cgcccaggca uucguggcuc ggccugagcg aagacgcgcc     2400

agggggaagg gacacgacgc uagcauucgg cagguugugg gcgaaggucc agaggcacaa     2460

guauuugagc guagagggcu ggccgaucuu aaagaagccg accuggauag ggucaaggac     2520

ccggagcgca accaggcuau aaucgaagcg gugcgggcau ggauuuuggc cggccgaccc     2580

gcugaugccc cuccaagauc ucccagggga gacguuaucg ugaaacuccg cacugccccu     2640

gcaaagaaga ucaaaccagc uguccccgug cggggcggga cggcggauag aggagacaug     2700

guacguauug acguguucac aaggccgaau cggaagggcc gcgaugagug guaccugguu     2760

ccuauauauc cacaccaggu caugaaccga aaagccuggc cccauccucc caugagauca     2820

gugguggcau acaaggacga agcugguugg accgagaucg gggccgagca caguuuuagg     2880

uucagccuau auccacgguc cuacguccag cugguuaaau cuaauggaga ugugauugaa     2940

ggcuaucucg ugucgaugga ccgcagcacu ggugcauuua gaguauucgu ccauaacaau     3000

ccugccgaga uggcuaaggg caucggggcc aagacccugc uuaacuugag gaaauuuaau     3060

guggaucgau ucggaguucg gacagaagug aaguccgagg uccguaccug gcacggcgaa     3120

guguguacuu cacccguucc accggacuga                                      3150


<210>  2630
<211>  3150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2630
augcagcuga gcaagcucaa cuacauccuu ggccuggacu uggggauugc cuccguggga       60

uggucuguca uagagaucga ugaaaccgag augcccauua gauuaaucga cguuggugug      120

aggacauucg aacgggcuga gguaccuaaa acuggcgaau cacuggcacu agcccgccga      180

cucgcgcgua guacgagaag gcugauucgg cgcagagcuu uuaggcuucu gaaggccaaa      240

cgguugcuca agaaucacca aaucgugagc gcagaugagc uguccacccu gccaaaccau      300

ugcugggagc uccgcgucaa aggguuagac gaacgacuga caugucagga gugggccgcu      360

gugcuuuugc aucugcuaaa gcacagagga uaucucucuc agaggaaguc ggaagcacag      420

aaugccgaua aagagauggg cgcucugcuu ucagguguug ccaacaauca caagcuguug      480

agcgagaacc auuaccggac uccggcggaa auagcaguga aaaaguucgc ccucgaggaa      540

gggcacaugc guaaucaacg cggagcuuau acccacacau uuaacagacu ggacuuacug      600

gccgagauga ggcuccuuuu ccagacccag cgggaacugg gcagucccua cgcauccggc      660

gaguuggagc aggaauuuug caaacuacug auguggcaaa agccugcucu cucuggggau      720

gccauccuga agaugcuugg aaaauguacu cuggagccaa cagaauauaa ggcggccaaa      780

aauaccuaca cggcagagcg auucgucugg uugacuaagc ucaacaaucu gcgcauuuua      840

gaaaacggua gcgagagagc ucugaaugcc gacgagaggg cucuacuccu gaaccagccc      900

uaugaaaaag ccaaguuuac cuacgagcag gcacggaaac uuuugcagcu gaaugaagcc      960

gcucgcuuca acuccgugag auacggcaag gagaacgcgg aaaagucaac acucauggag     1020

augaaagcau aucaugccau caggaaggcu guagagacug ccgauaguag cgcaugggcc     1080

acccugaaaa cacaaccucu ucuguuggac gaaauuggga ccgcuuuuuc uaucuacaag     1140

acggaugagg acauaaaaac ucagcuccag ggaaagcugg ccgauaaugu uauugacgug     1200

uuacuggaaa accuaaauuu cgacaaguuu auccaacucu cccugaaagc acuuuauaag     1260

auuuugccac ugauggagca gggccuccgu uacgaugagg cgugcucaca gaucuauggu     1320

gaccacuucg ggaaaaagga acagaaaaac gaucggcugu uaccccaaau cccugcugac     1380

gagauucgaa auccagucgu gcugagaaca cuuagccagg ccaggaaggu gauaaacgca     1440

gucauccgcu uguacggaag uccggcccgg auucauaucg aaaccgcuag agagcucggc     1500

aagucuuaua aagauaggaa ggaaauagag aaacgccagg agcagaaucg agccgaacgg     1560

gagaaggcag uugacaaauu uaaggaacug uuccccaacu ucauuggcga gccuaaggcu     1620

aaagauaucc uaacuaugcg ucuguacgaa caacagaaug ggcagugucu cuacuccgga     1680

aagagccugg accuucacag auugcuggag aaagguuaug uggagaucga ucacgccuua     1740

ccauuuucga ggacauggga cgauucuuuc aacaauaagg uacucgugcu ggcgggcgaa     1800

aaccagaaua aauccaacca aacccccuuu gaguggcuug ggaaggacga acagaaaugg     1860

gcccuguuca cggcucgcgu caauucaugc cgguuuccuu acaguaagaa gcagagaauu     1920

uugaccaaaa agcucgacga ugagggauuc auccagagga accugaacga cacacgauau     1980

auugcacgcu uucuagugaa uuucguuaac gauaaucugc aucucacugg caaagguaag     2040

cggagagugu uugccagcaa cgggcaaaua accgcuuucc ugaggggccg uuggggacuu     2100

acaaaagucc gcgaagaguc cgaccggcac caugccuugg augcaguggu aguugcgugu     2160

ucuacuguga gcauccagca gaagauuacc agauuuguca aguaccaaga gggcaaucug     2220

cagacagccg aaaggaucga ccgagagacc ggggaaauca uuccauuaca cuuccccgau     2280

ccgugggccu uuuuccggga ggaggugaug auacgcaucu ucucagaaau gccucaggag     2340

gaccucguca uuaaacugcc cgauagacca caggcuaacc augaauuugu gcaaccucuu     2400

uucguuagua ggaugcccac ucggaagaug uccggacagg gucacaugga gacgaucaaa     2460

agcgcaaagc gacuggacga aggcauuucu gugucaaaag uaccacuaac acagcucaag     2520

cugaaggauu uggagaauau ggucaacaga gaccgcgaga ucgcucugua ugaagccuua     2580

aaagcaaggc ucaccgaguu uaaggaugac cccgccaagg cuuucgcgga accuuuuuac     2640

aagaaagggg gagugcaggu uaagagugug cgugucgagc aagugcagaa aagcggcgua     2700

gugguucgga augguaacgg ggucgccgac aaugcaucca uggugagagu ggaugucuuc     2760

auaaaaggcg gaaaguauuu ucugguucca aucuacacuu ggcaaguggc uaaaggcauu     2820

cuuccggaca gggccgugau cgccuacacc cccgaaaacg agugggagcu gauggaugac     2880

ucugcucagu uccaguuuuc gauuuauccu aaugauuuga ucaagcucac aaccaaaaag     2940

gcaauauucu uuggguacua uagcggauuc aaccgccuga cuggugccau uaauaucaaa     3000

gaacacgacc uacagauuuc caagggcgau aaggggguac aucugggacu uggcgucaaa     3060

cucgcgcugu cauuggagaa guaccaggug gacgaacugg guaaaaacau ccgaccaugc     3120

agacccacac aacggcagaa cguuagguga                                      3150


<210>  2631
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2631
augagcuccu acagauucuc uuuugaccug ggcaccaacu caaucgggug ggccguguuc       60

gagcucgauc ccaagacaag gccugucgcu cuugcagacu gcggaguucg gauuuuucca      120

gauggucgca auccgaaaga caagcagagu cuggccgugg cgcgacguga agcuagagcc      180

guaaggcggc gcagagauag guauuugaaa cggcgcagcg aguuaaugga acgacuggug      240

gcaaagggcc uaaugcccga guccagagac gaucaaaaag cccucgaagg gcuggacccu      300

uacgcucuua gggcagccgg acuggaugac gcuuugccac cccaccauuu cggccgggcc      360

cucuuucacc ugaaccagcg ucgcgguuuc aagucuaaua gaaagacuga uaggggagag      420

aacucggaga cggggauaac caaaaucggc augaagaaac ugcaugaagc gcucuuauca      480

gagggcuguc ggacacuggg ggaacuucag caccgacauc acgaggcaaa ucagacuccu      540

gccauggaca ccaacccaaa gagcguccgc aguaaauuga gaggagaggg ugcuaaggcc      600

guguaugauu uuuaccccuc cagggacgca guugaagacg aguucaauaa gcugugggau      660

ucucaaaaac gguuucaccc ugaccuacuc acagauagcg cucgcgacgc cauuagagau      720

gcgaucuucu aucagaggaa gcugaaaacc ccagacgccg gccguugcuc cuuuaacccg      780

gcagaagaac ggguggcucg agccaugccc auugcucagc auuucagaau ccuucaguuu      840

gucggggauc ugagguugau agacaaugau cucgugcgcc acccucugaa caaugaccaa      900

aagaaagccu uauaccggga ucugcucgca aagggaaaac uuacuuucaa ggccaugaga      960

aagacaaucg gcauugguuc agaauaugcu uucagucaug agagcgagaa acagucugaa     1020

uuucgcgggg acgagaccgc ggcaaggcug gccggaccaa aguacuucgg ccccaaaugg     1080

cgagaauugu ccuuuaacga ccagucagag aucguagauc ggcuacugca cgaagagaau     1140

gaggaaacgc ucauucagua ccugcaagac cguuucggcu uugaucacga cagaugugag     1200

guuaucgcug augccccucu uccagacggg cauacuaggc ugggaaagac cgcauuggaa     1260

acacucgugg agauaaugcg cgaugaccau cugcccuaug aucaggccug cacuaaagcu     1320

gguuacgaac acgccuuacc gccugacggc gucauucugg augaccuacc cuauuacggg     1380

aucgcacucg agcggcacgu ggcguucgga gauggcaaga gcgagaaccc agaaaagaga     1440

uaugguaaaa uugcuaaucc uaccgugcau aucgcccuga accagcuuag gaaggucauc     1500

aacgcauuga uugcccgaua cgggcacccc gaccaaguug ugauggagau agcucgcgac     1560

cugaaacaga guaaggccaa acgggaugac aucucuaagg aacagaagaa aaaucaggau     1620

agaaacgacc aaaggcguaa gaaacuccag gaggcacaca uugcuguaaa ugccgagaac     1680

augcugcgca ugcggcuuug ggaggagcug gaugaagaca augcggccgc uagagcaugu     1740

ccauuuacag gcgagaccau cuccauggcc agguuguuca gccccgaagu ggagauagag     1800

cauauucucc cuuuuucgcg aucucuggau aacuccccag cuaauaagac guuaugcaug     1860

gucugggcca accgggcaaa aggaaaucgc acuccguggg aagcguucgg cagagacagg     1920

gagcccgggu uugauuggcc ugaaaucgcc gcucgggccg cacgacugcc aaagaacaag     1980

ucauggagau ucggacccga cgcuauggag cgccuagaaa gggaggguga uuuucucgac     2040

cgucagcuga cagauaccca guaucuuagu cggaucgcca gagaguacgu gacuaaaauu     2100

ugugacccua aucaaguuug ggugacacca ggcagguuga ccgcauggcu gcgccgaaac     2160

ugggggcuca auggaauccu gcccacgggc aacgccacca agaauagaga cgaucaccgg     2220

caucacgcua uugacgcgau agucgugggu uuaacagaua gggggauccu gaaccgcgua     2280

agccguuccg ccgcacuugc ugaagccgag gacuuggaua gaucuauugc cgaaaugccg     2340

gacccuuucg auggcuuccg ggacaacauc aggcacaucc ucgagcagau uauaguuagc     2400

cauaaaccag augcuggauu ugugcccggc cacccuaagg ggaauacuac ccccggagca     2460

cugcauaacg acacagccua cgguaucauu aaaacugaug cgaagggcau gucagaaguc     2520

gugacccgca aagacguccu aaguuucaag gggacaaagg agaucgaguc cauucgagac     2580

gcugccacca gacagagccu gcucgaugca guggccggag uuccaucugg ccuggcuuuu     2640

aaagaagcac uucgggacug gucagugagg acugguacga agcgcguaag agucacauuc     2700

aauaugagug ugaucccuau acaggauagg gacgggaaag ccuauaaggg cuacaaagga     2760

gauggcaacu ugugcgcgga gaucuaugaa cggcccgacg ggaagugggg agaugagauu     2820

aucagccguu uugacgcugu ucaaccaggc uucaccccgc aguggcagcg cgaucacccu     2880

gccgcacauc gagugaugag acuguuuagg gacgauauga uuauggccga gaucgacggu     2940

gucaugacug ugugucggau aguaaagaug ucccccggga aagugguuuu caccccacac     3000

cacgaagcua auacagauuc ucgcaacaga gacaaggacg aucccuuuuc guuccauacc     3060

ugcagcggag aguccuuaag gaaacggaag gcccagaaac ucuuugucga cccucugggc     3120

aagauucgag guuacagagc aaaggaaggc uga                                  3153


<210>  2632
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2632
augggcgaga acaugaucga cgaaagccug accuucggga uugaucucgg aauagguucc       60

ugcggcuggg ccgugcuuag aaggcccucu gcuuuugggc ggaagggagu caucgagggc      120

auggguucau gguguuucga cguuccugaa acaaguaaag agcgcacucc aacgaaucag      180

auucgacgua gcaacagacu guugaggcgg gugauccgca gaaggcggaa ucgcauggca      240

gccauucgaa gauuacugca cgcggcuggg cuacucccgu ccaccgauuc ugacgcccug      300

aagaggcccg gacaugaucc uugggaacuu cgggcacgug gccuggacaa accauugaag      360

cccguagagu uugccguggu ccucggccac aucgcuaaac gcagaggguu caagucggca      420

gccaagagga aagcuacaaa cauaucaagc gaugacaaga aaaugcugac ugcccuggag      480

gcgacccggg aacgacucgg acgcuacaga acagugggug agauguuugc aagggauccu      540

gacuucgcca gucggcgcag aaauagggaa ggcaaguaug aucguaccac ugcucgggac      600

gauuuagagc augagguuca cgcccuguuu gcagcucaac gaagacuugg gcagggauuc      660

gccuccccag aauuggagga agcguuuaca gccucugcau uccaucagag gcccaugcag      720

gacagcgagc gccugguggg cuuuugcccu uucgaacgga ccgagaaaag agcugccaag      780

cuaacgccau ccuuugagag guuccgccuc cuggcucgac uucugaacuu gcggaucacu      840

accccggacg gugaacgucc ccucacaguc gaugagauug cccuggugac uagagacuua      900

gggaagaccg caaaacuguc aaucaagagg guacgcacac ucauuggacu ugaagauaau      960

caacgguuca ccacgaucag accugaggac gaagauaggg acauaguugc ccgaacuggc     1020

ggcgcuauga cagggaccgc gacucugcgc aaagcauugg gagaggcccu auggacagau     1080

augcaggagc ggccagaaca gcuggacgcu auugugcagg uccucagcuu uuucgaggcc     1140

aacgaaacca ucacggagaa gcugagagaa auuggucuua cccuggcagu guuggaugug     1200

cuccugacag ccuuagacgc uggcgucuuu gccaaauuca agggggcagc gcacaucagc     1260

acuaaagcug ccaggaaucu gcuaccccac cucgagcaag gacgucgcua cgaugaggca     1320

uguaccaugg ccggcuauga ccaugcugcc ucucggcugu cccaccaugg ucagaucguu     1380

gcaaagacac aguuuaacgc ucuugugacu gaaauugggg agucaauagc caauccuauc     1440

gcgagaaagg ccuugauuga aggccugaaa cagaucuggg cuaugaggaa ccacugggga     1500

cucccaggca gcauacacgu agagcuggca cgagacgugg ggaauaguau ugagaagcgg     1560

cgcgaaaucg agaaacauau cgaaaagaac accgcccuua gagcuaggga gcggcgagaa     1620

guccacgauc uguuggaccu cgaggaugug aauggagaca cacuguuaag auaccgccug     1680

uggaaagagc aagguggcaa gugccuauau accgggaagg ccauucauau caggcagauu     1740

gcagcgacug auaacucugu ucagguggac cacauacucc ccuggucccg uuucggagau     1800

gacagcuuua acaauaaaac gcugugucuu gccucggcua accaacagaa gaaacggucu     1860

acaccguacg aaugguuguc cggccagacc ggugaugccu ggaaugcauu cguccagaga     1920

aucgagacua acaaggaacu gaggggguuu aaaaagcgca auuaucuccu gaagaacgcu     1980

aaagaggccg aagagaaguu ccgaucaaga aauuuaaacg acacccggua cgcagccagg     2040

cuguuugcug aggcggugaa acuuuuguac gccuucggcg aacgccaaga gaagggaggc     2100

aaucguagag uauuuacacg gccuggggca cucaccgcug cccugaggca ggccugggga     2160

guugaaaguc ugaaaaagca ggaugguaag cgcauuaacg acgaucgaca ucacgcucua     2220

gacgcacuca cuguggccgc ggucgacgag gcugaaaucc agagacugac aaaaaguuuc     2280

cacgaguggg agcaacaggg ccuugggcgg cccuugaggc gcguggaacc accuugggag     2340

uccuucagag ccgaugucga agcaacguau cccgaggugu uuguugccag gccagagcgg     2400

cgucgcgcuc gaggagaagg ccaugcagcc accaucagac aggugaagga gagggaaugc     2460

acucccauug uauucgagcg gaaagcgguc ucuagccuga aggaagcuga cuuagagcgc     2520

auaaaagaug gugagagaaa ugaagccauc guggaggcaa uuaggucaug gaucgccaca     2580

gggcggccug cugacgcccc accgcgaagu cccagaggcg auauuaucac caagauacgc     2640

cucgcaacaa cuaucaaagc ugccguuccu gugaggggag gcaccgcggg gcggggagaa     2700

augguccgug ccgacguguu uuccaagcca aacagaaggg gcaaggauga gugguaccug     2760

guacccgugu auccucacca gauuaugaau cgcaaagcau ggccaaagcc cccgaugcgg     2820

agcaucguug cuaacaaaga cgaagaugag uggacggagg ucgguccuga acaucaauuc     2880

cgauuuucuc uuuacccaag aucaaauauu gagaucauaa ggcccagugg ggaagugauu     2940

gagggauauu ucgugggccu gcaccgcaac accggugccu ugacaaucag cgcucacaac     3000

gacccuaagu ccauucauuc uggcaucggg acuaaaaccc uccuggcaau aucgaaguac     3060

caggucgauc gguuuggaag aaagagcccc guucguaaag aagugaggac auggcacggc     3120

gaggccugua ucuccccaac uccucccggg uga                                  3153


<210>  2633
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2633
augaagaucc ugggcuucga cauugggaua gccagcaucg gaugggcuuu uguggagaac       60

ggugaacuca aagauugcgg cgucagaauu uucaccaagg cagagaaucc caaaacaggg      120

gacucccuug ccaugccuag gcgggaagcg cgcucuguuc gacguagacu ggcuaggcgg      180

aagggacgcu uggagacuuu aaaaagacug cuagccaagg aaugggaucu cuguuacgag      240

gacuauaucg cagccgaugg cgagcugcca aaggcuuuua uggguaaaaa ccuuaauaac      300

ccguacgugc ugagguauga agcauugcag cggcuccugu caaaggagga acugguacgc      360

gugguccucc acauugccaa acaucgaggg uacggaaaua agaacgcuaa aaucacgaag      420

agugaggaga gcaagagaga acaaggcaaa auauuauccg cccuggcgac caaugcaucu      480

gccaucgcua gguaucggac agugggcgag uacuucuaua aggaauuuug cgagguuauu      540

aaaaaccccc aggggcuuuc gacuaaugaa cacuacaccc agucaaaggu gcgugucuug      600

aaaccuaucc gcaacaaggg aggugaguac acaaauugua uucugcagga ggaccuacaa      660

agagaacuca ggugcaucuu cgaguaucag aaaggcuuug gguucccaau aacccaggaa      720

uuucaggaua agauucugaa gaucgccuuc uaccaacggc cccuuaaaga cuuuagccau      780

cuggugggaa aguguacuuu cuauccugau gaguuucgag cauugaaaug cagucucucc      840

gcuguagagu ucauuacacu gucuaagacc aucaacguuu uaacguacau cagcaaagaa      900

uccggcgagg uguauucaaa gggucaguac gaacagaugc uggacagugu cuucaaggcc      960

gugugugaga aagggagccu cucuuuugcg cagcuucgca agauucugca cauagaugaa     1020

agaaucaggu ucaaagaggu ggacuauaug gcccggaagu ccaaaaauac uucaggagau     1080

agcacccaua agcaaaagac aaguaaccca gaaacuaaaa aguuuauuga gcacaaaaau     1140

uugaagaaau ucugcgagau ccuaucugaa auagcagagg accgcgcucu ggccgacucc     1200

auugcuagag auaugacccu caucaaggac gaagagaagc uggcccagaa acuucacggc     1260

uacggcaagu uuagccagga gcaaaucaca cagcugucgg cauugaacuu cucucaucac     1320

auuaaccucu cccugaaagc cuuaucagaa auccugcccu uuaugaggga ggggaugcgu     1380

uacgaugagg cuuguaagcu agcgggacuc aaagcacggc auaaugacaa gaaaaguaag     1440

uuccugccuc cauuuugcga aagcauuuau gccgaugaac uuaccaaccc ggucguucac     1500

cgagcuauag ccgaguacag aaagaucuug aaugcacuga uugccaaaua ugguuccgug     1560

cacaagaucc auaucgagcu cacgagggac guaggcaaaa acuuccagga acgcgagaag     1620

uacaaaaagg aaauugaguc uaauuauaag gcucgggugc aggccaugca agaaugugag     1680

aaacuggggc uuacucugag cgagggaaac auauugaagc ucagacuguu uagggaacag     1740

aaugagaucu gcgucuacuc aggccgcaaa auuacauuag caaaccugaa ggaacagggu     1800

gcgcuagaga ucgaucacau ucuccccuau agucgaucca gcgacgauuc uuacaugaau     1860

aaagugcugg uuuucaccaa cgaaaaucag aacaagggga auaagacucc uuacgaggcu     1920

uuuggcggag acucacaaaa auggggcgag aucgaaaguc uugccuugcg gagcggguau     1980

ccaaagaaaa aggcaaaacg uauacuggau aagggauucg gugacagaga ggccggcuuc     2040

aaguccagga acaucgugga cucuggguac auugcucgcc ucaucgccaa uuauacaaaa     2100

gaauaccuga aguuuuuacc ccuggauucg caugagaaca ccgcacuuau ugcuggagag     2160

aaagacagca agauccacgu cgaagccgug aaaggcaugu ugacggcgac caugcggcau     2220

uucugggguc ucggguccaa gaacagauau gagcacacac accaugccgu agaugcuaua     2280

auuaucgcau acauuaaugc cgcuaugauc cagagguuuu cacaguuccg acagaaccaa     2340

gaaagucuga aagcccgcuu uuaugcaaag gagcuagcga aggaagaguu caaaacucag     2400

cgggccuuuu ucgagccuuu uagaggcuuc agggaacagg uucuggcuaa ggugggacag     2460

auauuugucu cucguccacc ccgcaaacgg gccagaggcg cacuccacga ggaaaccuuc     2520

uacagcaucg acgauaagaa acuguccgag acauacgggg gaaaggaagg ugugcaaagg     2580

gcucuuucau ugggcaagau ucgacagauc gggacuaaaa uugucgccaa uggaccgaug     2640

gugcggguug acaucuuuaa gcauaaacag agccgcagau ucuaugcagu gccuauauac     2700

accauggauu ucgcccuggg cauuuuaccc aacaaggcug uagucucugg uaaagacaca     2760

aaguccaagg ugaucaaaga uuggcucgag auggacaguu gcuaugaguu uuguuucagc     2820

cuguuuaagg augaccuuau cgaaguucaa aaaucugaua uggagaagcc agaacuggcg     2880

uacuucgugu ccuuggacgc cucagacggg aggauuaaag uccggcacca cugcaaugau     2940

aucaccaaga uuaacgagaa ucagaagaaa cucuuuagug aagcaauaga gaaggaggug     3000

guaggccgag gaagcaucca gaaccugaaa guguucaaga aauauaaggu uucgccucua     3060

ggcgaaauua aggaggcuaa auacgaaccc agacagccaa ucgcccugaa gacuuccccc     3120

aaaaagcauc gcaaacaaaa uaacgcccag uga                                  3153


<210>  2634
<211>  3156
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2634
augaacaauu acauccuggg ccucgacauu gggauaacca gcgugggaua ugguaucguc       60

gauuccgaca caagagagau uaaggaugcc ggcguuaggc uuuuccccga agcuaacgug      120

gacaauaacg aggggcggcg cucuaaacga ggagcacgua gacugaagag gcggcgcauc      180

cacagauugg auaggguaaa acauuuacug gccgaauacg accuacucga ucugacuaau      240

auuccuaagu caacgaaccc auaucagacc cgggugaaag gccuuaauga gaagcugagu      300

aaggacgaau uggucaucgc gcuccugcac auagcuaaac gccgagguau ccauaacgug      360

aauguuauga uggaugacaa cgauagcggg aaugagcugu ccacaaagga ccaacucaaa      420

aagaacgcca aagcauuauc ugauaaguac gugugcgagc ugcagcuuga aagauuugag      480

caggacuaua aggucagggg agagaaaaau cgguucaaga cugaagacuu ugugcgugag      540

gcccgcaaau ugcuggaaac ccagucgaag uucuuugaga uugaucaaac auucaucaug      600

agauacauug aacuaaucga gaccaggcgg gaauauuuug agggcccggg caaaggguca      660

cccuucggau gggaggguaa cauaaagaaa ugguuugaac agaugauggg ccacuguacu      720

uacuucccug aggaacuccg aagcguaaag uacaguuauu ccgcugagcu guuuaaugca      780

cuuaacgacc ugaacaauuu gguuauuaca cgcgaugaag acgccaagcu caacuacggg      840

gagaaauucc agaucauuga gaauguguuu aagcagaaaa agaccccaaa ccugaaacaa      900

aucgcuaucg aaauuggagu ccaugagacg gaaauaaagg gcuauagagu gaauaagucu      960

gguaaacccg aguucacuca guucaaguua uaccacgauc ugaaaaacau cuuuaaggac     1020

ccuaaauauc ucaaugauau ucagcuuaug gacaacaucg ccgaaauaau uaccaucuac     1080

caggaugcgg agagcaucau uaaggagcug aaucaauugc cagaacuacu guccgagagg     1140

gaaaaggaga aaaucucagc acucaguggg uauagcggaa cacaccggcu gucucuuaag     1200

ugcauuaacc uguugcucga cgaucugugg gaguccucau uaaaucagau ggaacuguuc     1260

acuaaacuaa accucaagcc caaaaagaua gaccugagcc agcaacauaa gaucccuagu     1320

aaacuugugg acgauuuuau uuugucucca gucguuaagc gcgccuucau ccaguccauc     1380

caggugguaa augcuauuau agacaaauac ggccugccgg aggauaucau uaucgaacuc     1440

gccagagaga acaauagcga cgauaggcgu aaguuucuga accagcuuca aaaacagaac     1500

gaagagaccc ggaaacaggu ggagaaaguc cugcgagaau acggcaauga caacgcaaag     1560

agaauugugc agaagaucaa auugcacaau augcaagagg ggaagugucu cuauucgcug     1620

aaagauauac ccuuagaaga ccugcuaagg aacccucauc acuacgaggu ugaucacauc     1680

auuccacgcu cuguggcuuu cgacaauucc augcauaaca agguccucgu gcgggccgau     1740

gaaaauucaa agaagggaaa cagaacaccc uaucaguacc ugaauaguag cgaguccucu     1800

cuuagcuaua acgaguuuaa gcagcacauc uugaaucugu caaaaaccaa ggacaggauu     1860

acgaaaaaga aacgcgaaua ccuccuggag gaacgagaua ucaacaaguu cgacguacag     1920

aaggaguuua uaaaucggaa cuuaguugac acucguuaug cgacaagaga acugaccagu     1980

cuuuugaaag ccuacuucuc cgcaaauaac cucgauguga aggucaaaac uauuaacggu     2040

agcuuuacaa auuaccugag gaaggugugg aaauucgaca aggaucgcaa caagggcuau     2100

aaacaucacg cugaggacgc ccuaaucauu gcuaaugccg auuuucuguu caagcauaac     2160

aaaaagcucc ggaauaucaa caaaguccug gacgcaccuu cuaaggaggu ggauaaaaag     2220

agaguuaccg ugcaaucaga agacgaguac aaucagauau ucgaagauac gcagaaggcc     2280

caggcuauca aaaaguuuga gauuaggaaa uucagucacc gaguagacaa gaaaccaaac     2340

cgccaacuua ucaaugauac cuuguauagc acacggaaca uugacgggau cgaguacguc     2400

guggaaucca uaaaggauau uuauucuguu aauaacgaca aggugaaaac uaaguuuaaa     2460

aaggaccccc acagacuguu aauguacagg aaugauccgc agaccuucga gaaauuugaa     2520

aaggucuuca agcaguauga gucggagaaa aacccuuuug cgaaguacua cgaagagaca     2580

ggagagaaaa uccguaaguu cagcaaaacu ggccaagguc ccuauaucaa uaagauuaag     2640

uaccuccgcg aacggcuggg gagacauugc gacgugacca acaaauauau caacuccagg     2700

aauaagauug uacagcuuaa aauauacuca uaucgauuug auaucuacca guauggcaac     2760

aauuacaaga ugauuacaau caguuacauu gaccuggaac agaaaucuaa cuauuacuau     2820

aucagccggg agaaguacga acaaaagaaa aaggauaaac agauagacga uuccuauaag     2880

uucaucggau cauuuuacaa aaaugacauu aucaacuaua auggcgagau guaccgcgug     2940

auugggguua acgauagcga gaagaauaaa auccaguugg acaugauaga uauuucuauc     3000

aaggacuaca uggaacucaa caauaucaag aaaaccggag ucauuuauaa gacuauaggu     3060

aaauccacga cacacaucga gaaguacacc acugacauuc ugggcaaccu auauaaagca     3120

gccccaccua agaagcccca gcugaucuuc aaauga                               3156


<210>  2635
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2635
auggagaaca agaaucugca cuacauccuc ggccuugacc uggggauugc cagcguggga       60

ugggcugucg uugaaauaga ugagaaagaa aaccccuuga gauuaaucga cgugggugua      120

aggaccuucg agcgggcaga aacacagaag ggcgaguccc uggcccuauc ucgccgauca      180

gcgcguagug cuagaaggcu cacucaacgg cgcguggcca gacugaaaaa ggcaaaaagg      240

cuucuuaaga gcgagaauau uuugcucucc acggaugaac ggcugccuca ucaggucugg      300

cagcugcgcg uggaggggcu cgaccgaaag uuagaaagac aggagugggc cgcuguucug      360

cuucacuuga ucaaacauag gggauaucug ucucaacgga agaacgaguc gaaaucagaa      420

aauaaggagc uaggcgcacu ccugagcggu guggccagua accacgaacu ucugcagcag      480

gcuaccuacc guacaccagc cgaguuggcg gucaaaaagu uugaagugga ggaggggcau      540

auucgcaauc agcaaggagc auauacucac accuucucca gacucgaucu guuagccgaa      600

auggagcugc ucuuuucuag gcagcagcac uucggcaacc cguuugcuag cgaaaagcug      660

cuggagaauu ugacagcccu acugaugugg cagaaacccg cacucuccgg cgaagacauc      720

cugaagaugc uugggaaaug caccuucgag gaugaguaca aggcugccaa aaacacuuau      780

acagcggaac gguuuguaug gauaaccaag cugaauaacu ugcgaaucca agagaaugga      840

cucgaacgcg cccugucaga caacgagaga uuaaugcuga uugaacagcc uuacgagaaa      900

gcaaagcuaa cguaugcuca gguuaggagu auccucaauc ugagcgauga cgccauuuuc      960

aagggugugc gguacucugg cgaggauaaa aaggcuaucg aaacuaaaac cacacuuaug     1020

gagaugaagg ccuaccauca aauacgcaaa gucuuggaag ggaacaaccu gaaggcagag     1080

uggguggccc ucaaggcuaa uccaacucug cuugacgaga uuggaaccgc guuuucccug     1140

uauaaaacag augaagacau cucagcauac uuggccggca agcucagcca gcccgugcug     1200

aacgcuuuac uggagaaucu aaguuucgac aaauuuauuc agcucucucu gaaggcccuu     1260

uauaaauugc ugccucucau gcagcaaggu cugagauacg augaagcaug uagggagauc     1320

uauggggacc acuacggaaa gaagaccgaa gagacgcauc acuucuuacc acagaucccc     1380

gccgaugaga uucguaaccc ugucguucug cggacucuua cacaggcucg aaaagugaua     1440

aauggcguag ugagauugua uggcucccca gccaggaucc acauugaaac cgggcgcgag     1500

gucggaaaga gcuacaaaga ccggcaugaa cucaagaaaa gacaggagga aaacaggaag     1560

caacgcgaga agacuaucuc ggagauaaaa acacuguuuc cgaauuucuc uggugaaccc     1620

aagggcaaag auauucuaaa gaugcgacug uacuaucagc agaacgcaaa augccucuac     1680

uccgggaagc cucuggagcu ucaccgguug uucgaacaga aauaugugga gguugaccau     1740

gcgcugccau uaucacguac cugggaugac aguuuuaaua acaaggugcu cguccuggcu     1800

aaugaaaacc aaaauaaggg aaaccuuacg cccuucgagu ggcuggaugg caaaaauaac     1860

agcgagagau ggaggaccuu uaaggccuug guggaaacau ccgcauuccc uuacgccaaa     1920

aagcagcgca uccucucuca gaaacuggac gagaaggguu uuaucgaacg gaaccuaaau     1980

gauacuagau auguagcuag guuccugugu aacuuuauug ccgacaauau gcaccucacc     2040

ggggagggca agcgaaaagu uuucgcaagc aacggacaga ucacagcucu gcuucgccgg     2100

agauggggcu uggccaaguc aagggaggac aaugaucguc aucacgcgcu ggacgccgug     2160

uuagucgcuu gcaguacugu ggcaaugcaa cagaaaauua cccgcuuugu ccgguucaag     2220

gccggggaug uguuuacagg agaaagaaua gacagggaga ccggugaaau cauuccacuc     2280

cacuucccca cuccguggca guuuuucaaa caagagguug aaauccgaau cuucuccgau     2340

aacccuaagc uggagcuuga gaaucggcug cccgaccgcc cacaggcuaa ccaugaauuu     2400

gugcagccuc uauucguaag cagaaugccc acgaggaaga ugacaggcca ggggcacaug     2460

gagaccguca aaucugccaa gcggcucaau gaaggaauuu caaugauaaa aaugccacug     2520

acuaaguuga aacugaagga uuuagagcuc auggugaacc gagaaagaga gaaggaccug     2580

uacgaugcac uuaaaacccg ccuggaggcg uuuaaugacg aucccgccaa ggcuuucgcc     2640

gaaccuuuua ugaaaaaggg cggugcaauc guuaaaagug ugagggucga gcaagugcaa     2700

aagagcgggg uauuggugcg ucagggcaac ggaguugcug acaaugccuc caugguccgg     2760

guggaugugu ucacaaaaga cggcaaguau uuucucgucc caauuuacac cuggcagguu     2820

gcaaagggga uccugccgaa caaagccgug auucagggaa aggacgaaga ggauugggaa     2880

gacaucgaug acgcuacuuu ccaguuuucu cuacauccca augaucugau aucggugaaa     2940

acaaagaaag acgaguuccu ugguuacuuu aacggccuca acagacacac cgggggaauc     3000

aauauuagga cgcacgaucu ggaaaagagc aagggcaaac aggguaucuu cgaggggauu     3060

ggcguaaaga ucgcguuguc cuuugagaaa uaucagauag acgaacuggg aaagaacauu     3120

cgcuuaugua aaccuucaaa gcgacaacca gucagauga                            3159


<210>  2636
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2636
augaagaaca ccagccugaa uuacgugcuc ggccuugacc uggggaucuc cucugucgga       60

ugggccguug uggagauuga ugaaaaagag aaccccuugu cauuaauaga cguaggugug      120

agaacauucg aaagggcuga gacucagaag ggcgaaaguc uggcacuagc ccggcgccuc      180

gcgcgaagcg cucguagacu gacgcaaagg cgggucgccc gccuuaaaaa ggcaaaaaga      240

cuguugaagu ccgagaauau ccuccugucu accgaugagu gccugccuca ccaggugugg      300

cagcucaggg uugaaggguu agagcggaag cuggaacgcc aggagugggc cgcugugcuu      360

uugcaucuga uuaaacaccg aggauaucua ucgcaaagaa agaacgaguc aaaaagcgaa      420

aauaaggagc ucggcgcacu gcuuaguggu gucgccaaca aucauaaacu guugcagcag      480

acaacuuaca ggaccccagc ugaacucgcc gugaagaagu uugagguaga agaggggcac      540

auccggaacc agcaaggauc cuauacacau accuucucuc gucuggacuu acugagcgag      600

auggaacucc uguuuucccg ccagcagcac uucggcaauc cguuuacuuc agagaaacuu      660

uuggaaaacc uaacagcgcu gcucaugugg cagaagcccg cacugagugg cgaugccauu      720

cuuaaaaugc uggggaagug uaccuucgag aaugaauaca aagcugccaa gcacacguau      780

agcgcagaga gauuuaucug gauaacuaaa uugaacaauc ucaggaucca agagaacgga      840

cuggaacggg cuuuaaauga caacgagcga cuggcccuaa ugaagcagcc uuacgaaaag      900

gcgaaacuca ccuaugccca gguucgcucu auucugaacc uuuccgauga ggcaaucuuc      960

aaggguuuga gauacucagg cgaagacaaa aagacaagcg agacuaaaac cgugcugaug     1020

gagaugaagg cuuaccauca aauuaggcuc gcccuggaaa agaaugaucu uaaaacagag     1080

ugggcugaac ugaaggucaa caguucuuug cucgacgaga ucgggaccgc cuuuucccug     1140

uauaaaacgg augaggacau aagcacuugc uuagcaggaa agcugucgca gccagugcua     1200

aaugcccucc uggaaaaccu uaauuucgau aaauuuauuc aguugucucu gaaggcucuc     1260

cacaagaucc ugcccuuaau gcagcaaggc cugcgguacg acgaggcgug ugcagaaauu     1320

uauggugacc auuacgggaa aaagacagag gaaaaccacc uuuugcuccc ucagauccca     1380

gccgaugaga uccgcaaucc cgugguccug agaacccuaa cucaggcuag gaaaguuauu     1440

aacggagugg uacgucugua uuccaguccu gcccggauac acaucgagac aggccgagaa     1500

gugggcaagu cauaccagga cagaagggag cucgaaaaac gccaagagga aaaucggaag     1560

cagagagaga aggcaauuag cgagaucaaa acccuguucc caaacuuuuc cggggaaccg     1620

aaggccaaag auauacuuaa gaugagguug uauuaccagc agaaugcuaa augccuguac     1680

ucuggaaagc ccuuagagcu ccaucgccug cuugaacaag guuaugucga gauugaccac     1740

gcccugccuu ucagccgaac gugggaugac ucauucaaca auaaaguguu gguucucgca     1800

aacgaaaauc agaacaaggg caaccugacc ccauuugagu ggcuagaugg gaagaauaac     1860

agugagcggu ggaaagcguu caaggcucug guggaaacau ccgccuuucc cuacacuaaa     1920

aagcagcgua uccucagcca gaaacuggac gagaagggau ucaucgaaag aaaucuuaac     1980

gauaccaggu augucgcacg cuuuuugugu aauuucauug ccgacaacau gcaucugaca     2040

ggcgagggua agcggaaagu guuugcuucu aaugggcaaa ucacugccuu acucagagga     2100

agguggggcc uggcaaaguc acgagaggau aacgaccgcc accaugcucu ugacgccgua     2160

guuguggcgu gcaguaccgu cagcaugcag cagaaaauua cacgguucgu gagauuugaa     2220

gagggcaaug ucuucaccgg ggaaaggaua gaucgugaga cuggagaaau cauuccucug     2280

cacuuuccag cccccuggaa guucuuucgc gaggaggugg aaauccggau cuucuccgac     2340

aacccgaaac uagagcucga aaauagacug auugcuaggc cucaagcaaa ccacgaguuc     2400

guucagcccu uguuuguguc ucgaaugcca acgcggaaga ugacagguca gggccauaug     2460

gaaaccauaa agucggccaa acgccugaau gagggguuaa gcguaaucaa gaugcuccug     2520

acuaaacuua gacugaagga uuuggagucc auggucaaca gggaacggga gaaagaacuc     2580

uacgaggcuc ugaaggcccg acuagaagca uucaaugacg agccugcgaa ggccuuugcu     2640

gagcccuuca ugaaaaaggg aggcgccauu gugaaaucag uuagagugga acagguccaa     2700

aagaguggug ugcugguacg cgaugggaac ggcguggcag acaacgcuuc uaugguuagg     2760

gucgaugugu uuaccaaagg aggcaaguau uuccuugugc caaucuacac auggcagguc     2820

gccaaaggga uucuccccaa uaaggcaguu auccagggaa agaacgagga agacugggag     2880

gugauggaug acgccaccuu ucguuucagc cugcaccaga augauuuggu gaaacuggua     2940

acuaagaaaa agacaauauu ugguuauuuc aacggcuuaa aucgggcuac ggggaacauc     3000

gacauuaaag agcaugaucu cgaaaagucc aaggucaaag acggaaucca ccaaucaauu     3060

ggcaucaagc uggcgcuuag cuuugagaaa uaccaggugg acgaacuggg uaagaauaua     3120

agauugugua aaucuuccaa gaggcaggcc guucgcuga                            3159


<210>  2637
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2637
augaucggcg agcacgugag agggggaugc cuguucgacg aucauuggac ccccaacugg       60

ggugccuuua ggcucccuaa uacaguccgg acuuucacga aggcugaaaa cccaaaagac      120

ggcagcuccc uugcagagcc gcgccgacag gcccgugggc ugagaaggcg guugcgcaga      180

aagacccaaa gguuagaaga ucugcggcgc cuacucgcga aagagggagu ucugucucuu      240

ucagaccugg aaacauuguu ucgagagacu cccgcuaagg auccuuacca gcucagagcc      300

gagggccugg acaggccacu gaguuucccc gaaugggugc ggguacucua ucacauuacc      360

aaacaucgug guuuucagag caaucgcaga aacccugugg aggaugggca ggaaaggucc      420

cggcaagagg aggaaggaaa guuacugucu ggcgucggcg agaaugaacg acuuuugcgc      480

gaagggggau acagaacagc cggugaaaug cuggccaggg acccaaaguu ccaggaucac      540

cggcgcaaca gagcuggcga cuauucgcau acccuaucaa ggagccuccu gcuugaggag      600

gcacgucggc uguuucagag ucagcgaacu uuggggaauc cccacgccuc cucuaaccuc      660

gaagaggcuu uccugcacuu aguggccuuu caaaauccuu ucgcgagcgg agaagauaua      720

agaaacaaag caggccauug uucccuggag ccagaccaga ucagggcccc gcgccgguca      780

gcuagugccg aaacauuuau gcuccuucag aagaccggua aucugagauu gauucacagg      840

cgcacggggg aggagcgacc ccuaacugau aaagaacggg agcagaucca ucugcucgca      900

uggaagcaag aaaaaguuac ccacaagaca cugcguagac accuugagau uccugaagag      960

uggcuguuca cuggauugcc auaccauagg agcggcgaca aggcugagga aaaacucuuu     1020

gugcaccugg ccggcaucca ugagauacgc aaggcguuag acaaagggcc cgauccugcc     1080

gucugggaca cccugcgguc uagaagggau cuacucgacu ccaucgcaga uacacugacc     1140

uucuauaaga acgaagacga gauucuucca cgauuggagu cacugggacu cagccccgaa     1200

aaugcucgcg cccuggcucc gcuuaguuuc ucugguacgg cccaccuguc cuugagcgca     1260

cucggcaaac uguuaccuca ucuggaggaa gggaagucgu acacucaggc ccgggcugau     1320

gcgggauaug cagcaccccc accugacaga caccccaaac uaccaccccu cgaggaagcu     1380

gauuggagga acccuguggu auuucgugcc cugacacaga cccgcaaggu ugugaaugca     1440

cuuguccgga gauacggccc accguggugc auccacuugg agacugccag ggagcugucu     1500

caacccgcua aggugcgacg gcgcauugaa acagagcagc aggccaacga gaaaaagaaa     1560

cagcaagcag agagagaauu ucucgacauc guggguaccg cgccugggcc aggcgaucug     1620

uuaaagauga ggcuguggcg ggagcaggga ggcuuuuguc ccuacugcga ggaauaucuu     1680

aacccuacgc gauuggcuga gccaggguac gccgaaaugg accauauacu ccccuauucc     1740

agaucacugg acaauggaug gcacaaccgc guccuaguuc augguaaaga uaauagggac     1800

aagggcaacc guaccccguu cgaggcauuu gggggagaua cagcccggug ggacagacug     1860

guggcuuggg uacaggccag ucaccucagc gcaccuaaga aaaggaaucu gcuucgcgaa     1920

gauuucggcg aggaggcuga acgagaguug aaggacagaa accugacuga uacccgguuu     1980

auuacaaaaa cugccgcgac cuuacucagg gaccgccuga cauuccaccc agaagccccc     2040

aaggauccug ugaugacccu uaauggucgu cugacugcuu ucuugagaaa acaguggggg     2100

cuccauaaga accggaagaa uggcgaccug caccaugcac uagaugccgc uguccuggcc     2160

guggcaucca ggucuuucgu uuaccgccuc agcucacaca acgcggccug gggagagcug     2220

ccccgaggca gagaggcuga aaauggguuu agucuuccau auccugccuu ccgguccgag     2280

guguuggcaa ggcugugucc cacgcgcgaa gagaucuuac ucagacugga ccaaggaggu     2340

gucggcuacg augaggcuuu uaggaacggg cuucggccag uguucguaag ccgugccccg     2400

ucucgccgac ugagaggaaa agcacauaug gaaacacuaa ggucaccuaa guggaaagac     2460

caccccgagg gcccacggac cgccagucgc auuccccuca aggaccugaa uuuggaaaaa     2520

cuggagagaa ugguugguaa ggauagggac cggaaguuau augaagcucu ccgagagaga     2580

cuggcggccu ucgggggcaa cggcaaaaag gcauuugugg cuccuuuccg caaaccaugc     2640

aggagcggag aagggccccu uguccggucc cugcguaucu uugauucugg auacucgggc     2700

guggaguuga gagacggugg ggaggucuac gccguggccg aucacgaaag caugguuagg     2760

guggacguau augcuaagaa aaaucgcuuc uaccucgucc cuguguaugu ugcagaugug     2820

gcccggggaa ucgucaagaa ccgagcgauu guggcucaua aauccgagga agagugggac     2880

cugguagaug gcucauuuga cuucagauuu agucuauucc cgggugaucu gguggaaaua     2940

gagaagaagg acggcgccua ccuuggguau uacaaaucuu gucacagggg agauggccgc     3000

cuccuguugg accggcauga cagaaugcca cgugagagcg auugcgggac uuuuuauguu     3060

uccaccagga aggacguccu gucaaugagc aaauaccagg uggaucccuu aggagaaauc     3120

cggcucgugg gcucugagaa gccuccauuc guccuguga                            3159


<210>  2638
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2638
augaagagaa acuacauccu gggccucgac auugggauaa ccagcguggg auaugguauc       60

auugauuacg agacaaggga cgucaucgau gccggcguuc ggcuuuucaa agaagcuaau      120

guggagaaca augaagggcg ccgauccaag cguggagcaa gaaggcugaa acggcgcaga      180

aggcaccgga uucagcgcgu aaagaaauug uuauuugacu auaaccugcu aacugaucau      240

ucugagcucu caggcaucaa ucccuacgaa gcccgaguga agggucugag ucaaaaacuu      300

agcgaggagg aguucuccgc ggcucuguug caccucgcca aaagaagggg aguccauaac      360

gugaaugaag uugaagagga cacggggaac gagcugucua ccaaggaaca gauaucgcgu      420

aauucaaaag cacuggagga aaaguaugug gccgagcucc aguuagaacg gcugaaaaag      480

gauggcgagg uccgcggcag caucaacaga uuuaagacaa gugacuacgu gaaagaggcu      540

aagcagcuuu ugaaaguaca aaaggcauau caccagcugg aucaguccuu cauugacacu      600

uacaucgauc uacucgaaac caggcggaca uacuaugagg ggccuggaga agguucucca      660

uuuggcugga aagacauuaa ggagugguac gaaaugcuga uggggcauug caccuauuuc      720

ccggaggagc uucgaagcgu uaaauacgcc uauaaugcug aucuguacaa cgccuugaau      780

gaccucaaca aucuggugau cacucgcgac gaaaacgaga aguuagaaua uuacgagaag      840

uuucagauaa uugaaaacgu cuucaaacaa aagaaaaagc ccacacugaa acagaucgcg      900

aaggagauuc ucgugaauga ggaagauauc aagggauaca gagugaccuc cacgggcaaa      960

ccugaguuua cuaaccuuaa ggucuaucac gacaucaaag auauuaccgc aaggaaggaa     1020

auaaucgaga augccgagcu guuggaccag auugcuaaaa uccuaacaau auaccaguca     1080

agugaagaua uucaagagga acugacuaac cucaauagcg agcugaccca ggaagagauc     1140

gagcagaucu cuaaccuuaa ggguuauaca gggacccaca aucuguccuu gaaggccauu     1200

aaccucaucc uggacgaauu auggcauacg aaugauaacc aaauugcaau auucaaucgg     1260

cugaagcuag uuccaaagaa aguggaccuc ucacagcaga aggagauccc cacuacacug     1320

guagaugacu uuauucuuag cccugugguc aaacgcaguu ucauccaguc uaucaaggug     1380

auuaacgcua uaaucaagaa auacggauug ccaaaugaua uuaucauuga acuggccaga     1440

gagaagaacu ccaaagacgc gcaaaagaug aucaaugaaa ugcagaaaag gaaccgucag     1500

accaacgagc ggauagagga aaucauucga acuacaggca aggagaaugc caaauaucuc     1560

aucgaaaaga uuaagcugca cgacaugcag gagggcaaau gucuuuacag ccuggaagca     1620

auccccuugg aggaucuccu gaacaauccu uuuaacuaug agguugacca uauaauucca     1680

agaucggugu cuuucgauaa uuccuucaac aauaaggucu uagugaaaca agaagagaac     1740

ucaaagaaag ggaauaggac cccguuucag uaccugagua gcuccgacuc uaagaucagc     1800

uacgaaacgu ucaagaaaca cauucuaaac cucgcuaagg gaaaaggucg caucucaaag     1860

accaaaaagg aguaucugcu ugaggaacgg gauauaaaua gauuuagugu acagaaggac     1920

uucaucaaca ggaauuuggu ugauacacgc uacgccacuc gaggccugau gaaccuccug     1980

cgguccuauu uucgugugaa uaacuuagac gucaaaguga aaagcauuaa cgggggauuc     2040

accucuuuuc ugagaaggaa auggaaguuc aaaaaggagc gcaauaaggg cuacaaacac     2100

caugcugaag augcccuuau cauugcaaac gccgacuuua ucuucaagga guggaaaaag     2160

uuggauaaag cuaagaaagu cauggaaaau cagauguuug aggagaagca agcggaauca     2220

augcccgaga uagaaacaga gcaggaauau aaggagauuu ucaucacucc ucaccagauc     2280

aaacauauua aggacuuuaa agauuacaag uauagucacc ggguggacaa aaagccaaac     2340

agagagcuca ucaaugacac ccuguacagc acaaggaagg augacaaagg uaacacccua     2400

auuguuaaua accugaaugg gcucuacgau aaggacaacg auaaacugaa gaaacuuaua     2460

aauaaguccc ccgaaaaguu gcugauguau caucacgacc cucagacuua ccaaaaauua     2520

aagcucauca uggagcagua uggcgaugag aaaaacccac uguacaagua uuacgaagaa     2580

acgggaaauu aucuuacaaa auacucuaag aaggacaacg gccccgugau uaaaaagauc     2640

aaauacuaug ggaauaagcu gaacgcacac uuggauauua ccgacgauua cccgaacucg     2700

cgaaauaaag uagucaagcu cagccugaag ccuuaucgcu ucgacgugua ccuagauaac     2760

ggaguuuaua aauucgugac ugucaagaau cuggacguga ucaaaaagga gaacuacuau     2820

gagguaaauu ccaaaugcua cgaagaggcc aagaaacuca agaagauauc aaaccaggcu     2880

gaauuuaucg ccaguuucua caauaacgac cugauuaaaa ucaaugguga gcuuuaucgg     2940

gugauuggcg uuaacaauga uuugcugaac agaaucgagg ucaauaugau agacauuacc     3000

uacagggaau auuuagagaa caugaaugau aagcgucccc cacgcaucau caaaacaauu     3060

gcaucuaaga cccaaagcau caaaaaguac uccacugaca uacucgggaa ccuguaugaa     3120

gugaagucaa agaagcaucc ucagauuauc aaaaagggau ga                        3162


<210>  2639
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2639
augaagagaa acuacauccu gggccucgac auugggauaa ccagcguggg auaugguauc       60

auugauuacg agacaaggaa ugucaucgac gccggcguuc ggcuuuucaa agaagcuaac      120

guggagaaua acgaagggcg ccgauccaag cguggaacua gaaggcugaa acggcgcaga      180

aggcaccggu ugcagcgcgu aaagaaauua cuguuugauc auaauuuccu aacggacaac      240

ucugagcucu caggcauuag ucccuaugaa gcacgaguga agggucugaa ucaaaagauc      300

agcgaggagg aauuuuccgu cgcccuucug cacuuggcga aaagaagggg agugcauaac      360

guuaaugagg uggaagagga uaccgggaac gagcucucua caaaggaaca gauaucgcgu      420

aauucaaaag cucuggagga aaaguacguc gccgagcugc agcucgaacg guuaaaagug      480

gacggcgagg uacgcggcgc aguuaacaga uucaagacua gcgauuaugu gaaggaggcc      540

cagcaaaucc ugaaagucca gaaggcuuac caccaguuug accagaguuu cauugauacc      600

uauaucgacc uuuuggaaac aaggcggacc uacuacgagg ggccuggaga agguucccca      660

uuuggcugga aagauauuaa ggagugguau gaaaugcuga uggggcaaug cacuuacuuc      720

ccggaggagc uacgaucugu gaaauaugca uacaaugccg accucuauaa cgcucugaau      780

gaucuuaaca accuggugau cgcccgcgac gaaaaugaga aguuggaaua cuaugagaaa      840

uuucagauaa uugaaaacgu cuucaagcag aagaaaaagc ccacacucaa acagaucgcg      900

aaggagauuc ugguuaauga ggaagacauc aaaggauaca gagugaccag cacgggcaag      960

ccugaguuua cuaacuucaa gguauaccau gauaucaaau ccauuaccuc aaggaaggaa     1020

auauuagaga augcagagcu gcucaaccaa aucgccaaaa uucuuacaau cuaucagagu     1080

agcgaagaca uacaggguga gcuggcucgg uuguacucug aacuaacuca agaggaaauu     1140

gagcagaucu ccaagcuggc cggguauacc ggaacacacc gccucucacu gaaagcaauc     1200

aaucuuauua ucgaugagcu guggcacacc agcgacaacc agauggaaau uuuuaauaga     1260

uugaagcucg ugccaaagaa agucgaucug agucagcaaa aggagauacc cacgacuuua     1320

guggacgauu ucauccuguc uccuguugug aaaagguccu ucauucagag caucaagguc     1380

aucaacgcua uuauaaagaa guacggcuuu ccaaaugaca ucauuaucga acuagcccgu     1440

gagaagaacu cgaaagaugc gcagaaguuc auuaaugaaa ugcagaaacg gaaccgacaa     1500

acaaaugaga gaaucgagga aauaaucagg accacuggca aggagaacgc caaauaucuc     1560

auugaaaaga ucaaacugca ugacaugcag gaggggaagu gucuuuacuc uuuggaagca     1620

auuccccugg aggaucuccu gaacaauccu uuuaacuaug agguggacca caucauacca     1680

cgcuccguau cauucgacaa uaguuuuaac aauaagguuc uugugaaaca ggaagagaac     1740

agcaagaaag gaaaucggac accguuccag uacuuuuccu cuagcgauuc aaagauuagu     1800

uacgaaaucu ucaaaaagca uauucugaac uuggcuaagg guaaaggcag aaucuccaag     1860

aaaaagaaag aguaucuccu ggaggaaagg gacauaaauc gcuuuagcgu ccaaaaguac     1920

uucaucaacc gaaauuuagu ggauacccgg uaugccacgc gugagcugau gaaccuacuc     1980

agaucuuacu uuagggucaa ugaccuggau auuaagguga aaucaaucaa cgggggauuc     2040

accaguuuuc uucgcaagaa auggaaguuc aaaaaggaac ggaauaaggg cuauaaacac     2100

cacgcugagg acgccuugau uaucgcaaac gccgauuuca uauuuaagga auggaaaaag     2160

cuggacaaaa caaacaaggu uauggaggau cagauugugg aggaacagca ggcugagagc     2220

augcccgaaa ucgagacuga acaagaguac aaagagaucu ucauuacccc ucaucagauc     2280

caggacauua aggauuuuaa ggacuauaaa uacucccaca gaguagauaa gaaaccaaau     2340

agggaacucg ucaacgacac acuguacucu acucgaaagg acgauaaagg uaauaccuua     2400

auagugaaca aucugaaggg gcuuuaugac aaggauaacg acaaauugaa gaaacucauc     2460

aauaagucgc ccgagaaacu gcuaauguac caucacgauc cucaaacaua ucagaagcug     2520

aagguuauua uggaacagua cagcaacgag aaaaauccac ucuauaagua ccaugaagag     2580

accggcaacu aucugacuaa auacuccaag aaagacaaug gacccgugau caagaagauu     2640

aaauacuaug gcaacaaacu uaaugucuac uuggauauca cgcgcgacua ucugaaauca     2700

cggaacaaga uagugaaauu aagucucaag ccguacagau ucgauguaua ucuggacaau     2760

gggguguaca aguuuguuac agucaaaaac cuugauauca uuaagaaaga ggacuauuac     2820

gaaguggauu cugacagcua cgaggaagcg aagaucguga aaaaggucuc cgagcaggca     2880

caauucguug ccucauuuua uaacaaugac cugauuaaag uggauggaga guuguucagg     2940

gugaucggug uaaacaauga ccuccugaac cguauagaag ucaauauggu ggauauuacc     3000

uacaaggagu aucuagaaaa caucaaugac aagaaaagcu cucgcaucau uaagacuaua     3060

uccaguaaaa cccagagcau caagaaauac ucuacagaua uucugggcaa ccucuaugag     3120

gcuaagucca agcagcggcc ucagaugacc augaaagggu ga                        3162


<210>  2640
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2640
augaagagaa acuacauccu gggccucgac auugggauaa ccagcguggg auaugguauc       60

auugauuacg agacaaggga cgucaucgau gccggcguuc ggcuuuucaa agaagcuaau      120

guggagaaca augaagggcg ccgauccaag cguggagcag gcagacugaa aaggcggcgc      180

agacacaggu ugcagcgggu aaagaaauua cuguuugacu auaaccuacu cacugaucau      240

ucugagcugu cagguauuaa ucccuacgaa gcccgcguga aggggcuuag ucaaaagcug      300

agcgaggagg aguucuccgc ggcuuugcuc caccuggcca aacgaagagg aguccauaac      360

gugaaugaag uugaagagga cacgggcaac gagcugucua ccaaggaaca gaucucgagg      420

aauaguaaag cacucgagga aaaguaugug gccgaguuac agcuggaacg gcuuaaaaca      480

gauggcgagg uccgugggag cauaaaccgc uuuaagacuu cagacuacgu gaaggaggcu      540

aaacaguugc ugaagguaca aaaagaauau caccagcuag aucaguccuu caucgacacc      600

uacauugauc uccuggagac aagaaggacc uacuaugaag gaccugguga gggcucucca      660

uuugggugga aggacaucaa agaaugguac gagaugcuua ugggacauug cacuuauuuc      720

ccggaggaac ugcggagcgu uaaguacgca uauaaugccg auuuguacaa cgcucucaau      780

gaccugaaca auuuagugau ugcccgagac gagaacgaaa aacuggagua uuacgaaaag      840

uuucagauca uagagaacgu cuucaagcaa aagaagaaac ccacacucaa gcagauugag      900

aaagaaaucc uugugaauga ggaagauauu aagggcuacc gcgugaccuc cacggguaag      960

ccugaguuuu caaaccugaa agucuaucac gacaucaagg auaucacugc gagaaaagag     1020

auuauagaaa augcagaguu gcuagaccag aucgccgaaa uucugaccau cuaccagagu     1080

agcgaggaua uacaagagga acucguuaag cugaacucug agcuuacaca ggaagagauu     1140

gaacagaucu ccaaucugaa aggguauacu ggaacccaca acuugucacu caaggcuauc     1200

aaucugauuu uagacgagcu guggcauaca aacgauaauc aaauggccau cuucaacagg     1260

cuaaagcucg ugcccaaaaa gguagaccug agccagcaga aagaaauucc aaccacgcuu     1320

guggaugacu uuauauugag uccugucgug aagcggucuu ucauccaguc cauuaaaguu     1380

aucaaugcaa ucauuaagaa guacggccug ccaaacgaua uaaucauuga gcucgcucgc     1440

gagaaaaaua gcaaggacgc ccagaaaaug aucaacgaaa ugcaaaagag aaauaggcag     1500

acuaacgagc guauugaaga gaucauacgg acaaccggca aagaaaacgc gaaguaucug     1560

aucgagaaaa uuaaacuuca cgacaugcag gaggggaagu gucuguacuc guuggaagcc     1620

aucccccucg aggaucuguu aaauaacccu uuuaauuaug aaguggacca uauuauccca     1680

cgaucugucu ccuucgauaa cucauucaau aacaaagugc ugguaaagca agaggagaau     1740

aguaaaaagg gaaacagaac uccguuucag uaccuaagcu ccucugacag caaaauauca     1800

uacgaaacau ucaagaagca cauucucaau cuggcaaaag guaagggcag gaucaguaaa     1860

accaagaaag aguaucuuuu ggaggaacgc gauauuaacc gguuuuccgu ucagaaggac     1920

uucaucaaua gaaaccuggu ggauacgagg uacgcuaccc gcgaacucau gaaucuguua     1980

cgaagcuauu uucgggucaa caaucuggac gugaagguca aaucuauaaa cgggggauuc     2040

acaucauuuc uucguagaaa guggaaauuc aagaaagaga ggaacaaggg cuacaagcac     2100

caugccgagg augcuuugau cauugccaau gcagacuuua ucuucaaaga auggaagaaa     2160

cucgauaagg ccaaaaaggu gauggagaac cagacuguug aagagaaaca agcugaaagu     2220

augagcgaga uugagaccga acaggaguau aaggaaaucu uuauaacacc ccaccagauu     2280

cagcauauca aggacuucaa agauuacaag uauucccacc gcguggacaa aaagccuaau     2340

cgggagcuga ucaacgacac ucuauacucu accagaaaag augacaaggg uaauacacug     2400

auuguaaaca aucucaacgg gcuguaccau aaggauaaug acaaacuuaa gaaauugaug     2460

aacaagucgc cagaaaaacu guuaauguau caccacgauc cccaaaccua ccagaagcuc     2520

aagcugauca uggagcagua uggcgacgag aaaaauccuc uuuacaagua uuacgaagag     2580

acuggaaacu aucugacgaa auacagcaag aaagauaaug gcccagucau uaagaagaua     2640

aaauacuaug ggaacaaguu gaaugcgcau cucgacauca cagaugacua ccccaacucc     2700

aggaacaaag ugguuaagcu gucacuaaaa ccguaucgau uugaugugua ccuggacaau     2760

ggagucuaua aguucgugac cguaaagaac cucaauguga uuaaaaagga aaacuacuau     2820

gagguuaaua guaaaugcua cgagaaggcc aaaaagcuga aaaagaucuc ugaucaagca     2880

gaguucauug cuagcuuuua caacaaugac cuuaucaaga uagacgguga auuguaucgc     2940

gucaucggcg ugaacacuga ucugauuaau cggaucgaag ugaacauggu cgacauuacc     3000

uacagagagu auuuagaaaa uaugaacgau aaaagguccc cucguaucuu caagacaaua     3060

gccucaaaaa cccagagcau uaagaaauac ucuacugaca uccucgggac acuguaugag     3120

guuaauucca agaagcaccc ccagaugauc augaaaggau ga                        3162


<210>  2641
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2641
augagcggca ugguguucgg gaucgaccug ggaauugccu ccugcgguug ggcuguccuc       60

agacagcccc aaagggaugg cgacccuggg gagauaguug aucuuggauc uuggauguuu      120

gacgugccag aaaccgauaa ggagcggaca ccgacuaacc agguacgccg aggcaaucgu      180

cuguugagaa gggugauccg gcgcagagca cagaggaugg ucgaaauucg gcgcuuauuc      240

cacgaccaug gucugcuagc cgggcacgcg cccgaggcuc ucaaacgagc cggacuggau      300

ccuugggacc uuagagcaag gucacuggau aagguguugg aaccagccgc ucucgcaguu      360

gcccugggcc auaucgcuaa acggcguggc uuuaagagug ccgcgcgcag aaaagaggca      420

aacacggccg gggacgauca gaagaugcug aaggcucucg aggccaccca cgaaagguua      480

ggacgguacc gaacaauugg ugagauguuc gcacgcgacc ccgauuuuga aagcagaaaa      540

aggaaucggg acggcauguu cgaccgcacu caagggagag augaccugcu ucaugaggug      600

ggagagaucu uuaaggcuca gaggcguuug ggcuccgccc uggcgaccgc cgaacuagag      660

caggcauuca cagcuauagc cuuucggcag cucccuaucc aagauucuga acgacugguc      720

ggucuuuguc aguucgagcc aaaagaaaag agagcugcca gguuugcacc cucguucgag      780

cgcuuucggc uguugcagag acucaccaac cugagggugg uaacuguuga gggggaacgc      840

ccuuuaacag ccgacgagau ugcugcggca gccgcugauc ugggacgaac cgccaaacuc      900

ucagugaagg aaguccggaa gcguaucggc cuugcagccg accacagauu cgcugccuuc      960

aaagcagaug aggaagacag ggauauuauc gcgcgcacgg gcgaggcucu gcacgggacu     1020

uaucgguuga gaaaggcccu aggagagggu cugugggcag aaaugcuccc aggccagcug     1080

gacgccauag cucaugcccu uagcuuuuuc gagacccaag augugauucu gaaagaauug     1140

gacaagcucg accugccggc aggggugagg gaugcuaucg ccacaggauu agacgcgggc     1200

gccuuugcuc gauucaaagg uacugggcac auuagugcac gcgccgcucg ggcccugcua     1260

ccccaucucg aggcaggccu gagauacgau caggcgugca ccaaggccgg auaugaccac     1320

gcugccucca ggugggcaaa acgugaacag gucgcugaua aggccgcauu uaaucgccuu     1380

guuacagaca ugggcgccga gaucgcuaac ccuaucgcgc ggaagucuuu gaccgaggcc     1440

cugaaacaac ucugggcaau gagaaauagg ugggggcugc cagaugcuau ucacauagaa     1500

cuugcccgag acgugggaaa cagccuggag aagcggcgcg aaaucgagag agccauugag     1560

aaaaauacgg cugcaaggga acgggagcga ggugaagcca gagauuugcu cggcaucgac     1620

gauguauccg gggacacucu guuacgcuac aggcugugga aggagcaggc gggacguugu     1680

cccuauacag augcuccuau accacccggc gccauuaucg caaccgacaa cucauuccag     1740

guggaccaua uccuaccgug gagucgguuu ggugaugaca gcuucgccaa uaaaguccuc     1800

ugcgcuacug cagccaacca gagaaagaag gggcaaacac cuugugaaug gauuaccgcg     1860

gcucagggcg aggagggaug ggccacguuu guggcaagga ucgaaggcaa ugccgcuuuc     1920

cgcgggccca aaaagcgaaa cuacguucug aaaaaugcca aggaggcaga agagagauuu     1980

cgggcuagga accuuaacga uacccgcuau gccgcgcguu ugcuggccga agcagugaaa     2040

cucuucuacc cagagggaga gagacaggac aaggguggcg uccggagggu guuuacacgc     2100

ccugggggac ugacugcugc cuuacgacac gcuuggggcg uagaagcacu gaagaaaaga     2160

gaugguaagc ggguugacga ugccaggcau cacgcccuug acgcuuuggu ggucgcggca     2220

auuggggagg gcgaagugca gcgccucacc agaucuuacc aagaguggga acagcaggga     2280

cuggccaggc cccuacggcg ugucgaucca cccuggggcg acuuccauuc cuuccgccga     2340

gaggugaaag augcuuauga cgggauauuu guugccagac cugagaggcg gcgcgcaaga     2400

ggagaaggcc acgccgcuac aaucaggcaa gugcgggagc gagauggugc ggccguaguc     2460

uucgaaagaa aggcaauugc ugaccuguca gagaaacgcc ucgccgacau caaggaucca     2520

gagaggaauc aggcaaucgu ggaagccauu cggcagugga uaguugacgg gcguccggcu     2580

gauagacugc ccaggagccc ugccggagac gagauccgca aggugcggcu ucgaacuaaa     2640

ggcaagccag cgguccaggu gagagguggc gcagccgauc gcggggaaau uguaagggug     2700

gacguuuuua ccaaacccaa caagaaagga aagaaugagu ucuacuuggu cccuaucuau     2760

ccacaccagg ugaugaacaa agcugaaugg cccacaccgc cuaugcgggc cguggucgcu     2820

uacaaggaug agagugagug gacccuguua gacgaaaauu uuggcuuccu cuuuucucug     2880

uucccaagau ccuauguuga ggugacuaag cccgggggag aagugcuuag cggcuacuuu     2940

caagguaugg aucguucgac gggggcaauu ucucuguuca accauaggga cucccgguca     3000

uugacagaug acaguggaaa uagcacccgc ggcaucggug ccaaaacucu ccugaccaug     3060

aagaaauauu ccguagaccg auuuggcaag agagcugagg ucaaaucuga agugaggaca     3120

uggcacgggg uugccugcac cuacccuacu cccccaggau ga                        3162


<210>  2642
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2642
augaagcuga caccccucaa cuacauccuu ggccuggacu uggggauugc cagcguggga       60

ugggcugucg uugagauaga ugaacaggag aauccuuuag gucugaucga cgugggcgua      120

agaaccuucg auagggcaga aguggccaaa acuggggagu cccuagcgcu cgcucggcgc      180

cuggcccgau cuucacguag acuugucaag aggcgggcag accgcauuaa aaaggugaaa      240

agacuguuga agagugaaaa cauccuccug agcgccgaug agcaacugcc aaaugacguu      300

uggcagcuca gggugaaggg auuagaucag aaacuggagc ggcaggaaug ggcugcaguc      360

cuuuugcauc ugcuaaagca ccgcggcuau cucucccaac gaaagaacga gucuaaaucg      420

gaaaauaaag agcugggugc ccuuagauca gggguggcug ccaaccacca gauucugcag      480

agcgcggagu acaggacgcc ggcagaaauc gccguaaaga aguuucaugu ugaggaagga      540

cacauacgga aucagcgugg cgcuuauacc cauacauuca gucgcuugga ccuccuggcc      600

gagaucgaau uacuguuuac uagacaaacc gagcucggca acccccacac auccgcaaaa      660

cuucuggaga auuugaccgc ucuacugaug uggcagaagc cugcccucgc gggggaagcc      720

auucugaaaa ugcuuggaaa gugcacuuuc gagccaacag aauacaaagc agcuaagaac      780

ucuuauagcg ccgagagguu uguguggcug accaaauuga auaaccuccg gauccuggaa      840

cagggugcug agcgagccuu aacggauaau gagcgcuucg cacugcuaga ccagcccuac      900

gaaaaggcca aguuuacuua ugcucaagcg agaaccaugc ucgcacugcc ugaugaggcc      960

auuuucaaag gcgucaggua ccagggggaa gacaagaaag cuguggagac aaagacuauc     1020

cuuauggaaa ugaaagccua ccaccagaua cggaaggcau uggagaacgc cgaucugaag     1080

gcugagugga augaacucaa aaacaacucc gagcugcuug acgacauugg aaccgccuuu     1140

ucacugcaua agacagauga agacaucugu cgcuauuugg auggcaaacu cagugagagc     1200

auucugaaug cauuacugga gaaccuaaau uucgacaagu uuauccaacu cucucugaaa     1260

gcgcuucagc agaucuugcc acugaugcuc cagggucaaa gauacgauga agcugugucc     1320

gccauuuaug gggaccacua cggaaagaag ucagcagaga uaaacaggcu guuacccacc     1380

aucccugccg augaaauucg uaauccaguc guucugcgga cgcuuacuca ggcucgaaaa     1440

gugaucaacg ccguagugag auuguauggc agcccggcaa ggauacauau ugagacaggc     1500

cgcgaagucg ggaagaguua ccaggaccgg aaaaagcucg agaaacagca agaggauaau     1560

agaaagcaca gggaaucugc ugugcagaag uucaaagagu auuuucccaa cuucguugga     1620

gaaccuaagg guaaagacau ccugaagaug cgccuauacg agcugcagca ggccaaaugc     1680

cucuacuccg gcaagagccu ggaacuucac cgauugcugg agaaagggua uguggagguc     1740

gaucaugcgu uaccauucuc gcggaccugg gacgacucuu uuaauaacaa ggugcucgua     1800

cuggccaaug aaaaccaaaa uaagggaaac cuuacucccu acgaguggcu ggauggcaaa     1860

aauaacuccg aaccuuggca gaacuucguu gcucgugugc agacaucagg uuuuagucac     1920

accaagaaac agagaaucuu gagccauaag cucgacgaga aaggguucau ugaaaggaau     1980

cugaacgaua cgcgcuaugu cgugcgguuu cuauguaauu ucaucgcaga caacaugcug     2040

cucaccggca agggaaagag aaaagucuuu gccuccaaug gccaaauuac agcucugcuu     2100

agggggcgau ggggauugca gaaggugcgc gaugacaacg aucggcacca ugcccuggac     2160

gcaguugugg uagcgugcuc uacugucgcc augcagcaga aaauaaccag auucgugagg     2220

uacgaggagg guaauguuuu uagcggcgaa cguaucgauc gcgagacagg ggaaauuauc     2280

ccauuacacu uccccucacc gugggccuuu uuccgggaga acguggagau cagaauuuuu     2340

agugaaaauc cuaagcucga gcuggaaaac aggcuucccg acuauccaca auacaaucac     2400

gaguucgucc agccucuguu cgugucccga augcccacuc ggaaaaugac cggacagggc     2460

cauauggaaa caguaaagag cgcuaagcgc cuagaugagg gucucucugu gcugaaaguu     2520

ccauugaccc aacugaaguu aaacgaccuc gagagaaugg ucaauaggga acgggagaug     2580

gcacuguaug aaucacuuaa agugcgacug gagcaguuug auaacgaccc cgcuaaggcc     2640

uucgcagaac cuuuuuacaa aaagggcggg gccuugguga aggcugucag aguugagcag     2700

acucagaaaa guggagugcu cgugcgcgac ggcaaugggg uagcggauaa cgccagcaug     2760

gucagggugg acguuuucac gaaggcagga aaauacuuuc uggugccaau auauacaugg     2820

caagucgcua aggguauccu accgaacaaa gccgugaccg ccaauguuga ugagauugac     2880

uggcuggaaa uggaugaguc cuaccaguuc aucuuuacua uguaucccaa cgaccuugug     2940

aagguaaaac ucaagaagga agaguucuuu ggcuacuaug ggggacugga ucgugcuacc     3000

ggcgcaauug ucaucaaaga acacgacuug gagaagucua aaggucggca ggggauauac     3060

agaaucggcg ugaagcuggc cuuaucguuc gagaaauauc agguggauga acucggaaag     3120

aauauuaggc cuugucgccc aacaaagcga caacauguca gauga                     3165


<210>  2643
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2643
augagaaucc ugggcgugga ccucgggguc gccagcguug gaugggcucu ugugaacgau       60

aagaauaaca aaauuauaga cuccggugua aggaucuuca cccagucuac aaaucgggag      120

ggcaagacuc uggcagccgu gcgccgauca uuucgugggg gaagaagggu cuugagucgg      180

cgcaaacaaa gauuaaacca gcugaagaaa cuauucauug cgcacaagcu ccugagcaag      240

uccgaauuua aacaucuuuu caauucuaag aacaggcugg auaucuggca guugcgggcu      300

aaaggccucg agcagcaacu gaauaacaag gaaugggccc gcgugcugua ccacauugca      360

aaaagacgag ccuaucaguc gaauaggaag ucagaggaaa gcgguaacac ggacaagaaa      420

aagguucuca gugggaucuc caaaaaugag aagaaaauga acgaguuagg auacaagacc      480

auaggcgaaa ugcuguauac agagaaucgg uaccccgaua accguauccg cagaaauaaa      540

aaggaccagu auaagcauuc uauuagcagg gaucuuuugc ggaaagaaau cgacgugcug      600

uuugaguccc agcgagauuu cggcaaccaa uuugcuacug acgggcuaaa ggagcacuac      660

aaagauauug cauucaccca gcgcccuauc aaguuuaaag ccgaccucgu cggaaagugc      720

acauucgaaa cccaggagua uagaggugcu aagaacuguu uuucagugga aaagaguacu      780

cugcuuagca agauaaauaa cacagaucug auugacaaau cuaccggcga gaucaagagg      840

uuguucgaaa uuuccgaguu ugacaaacuc cuggauaucu ucuacucaau caaggaggua      900

aaguacacga cuuuacggaa acagcugaag auuaccgacg ccuucaaauu uaagcaacuc      960

gauuaucauc agaauuucga auacaaaaca aacauaaagg cgaagaucga ggacuuuuau     1020

aaacuugaag auuggcugac uccagagcag aagguuuugc uagaaaaaca agugcuggac     1080

gauaagaaag agaaguucaa augcuacaag uauagcagua uucgcucuau ccucucccug     1140

agcgagucgc agaaguuuaa uggggucaaa uacgacacca agguggaaaa cacaguguuc     1200

ggaaaacuua auggcuauca cucuauaaag aaagagcuga acaaggaagu cuuugauuug     1260

cuguucauug acaaggauaa auuuaauaag aucgcagaga uccuguccua ccagaaagac     1320

gacaagucag ccacccagga uuuaaagaag caaguuuuca acgacuuuaa uauggauaag     1380

acgauuguga acgaagcuau caauagucug cuaaacauua gcuucuccgg uuuuaauucu     1440

cucagccuga aagccauaaa caaacuuuug ccguaccugu gucaggggcu caaauaucac     1500

gacgcaguag cucauucaga guacaagcac cauagucagu ucaaagccgc gaacccccag     1560

caauaucuga gaccucuuaa uaaggaggaa aacuaccaga ucacuucccc aacagugaag     1620

agggucuuca gccaguuucg uaaagugcug aaugccguua uucggaagca cggaucuuuc     1680

gaugcaaugc acaucgagau ggcucgagaa uugaaaaacu caaagaaaau caaguuugag     1740

auucagaccg gccaaaaaga guaugcccag gaaaaggagg cuauaagagu gaaguuccuc     1800

gaaaucuuua aauacgaggg cagugggagg gaccuguuaa agcugcgccu auaugaacag     1860

cagcauggaa aaugcauuua cagcaagaaa gagaucgaga uuaauaagcu ccuggaagau     1920

gguuacgucg agaucgacca cauacuuccc uggucccgga cuuucgauaa cucuuugaau     1980

aacaaggccc ugugucucuc ggcagaaaau caaaacaaag ccaaucagac accuuuugag     2040

uaucugacca acagcgacaa aaauuccaaa gauucaaagg aauggcagga guacaaaaac     2100

uaugugaguu cuuucgcugg caucaagcaa gcgaagaaac auaaguuacu gaauacgacc     2160

cuuccaccca gaagggggaa cgacuuuagc gaugacauua aaaacccuga guccggauuc     2220

uuggcacgca aucucaacga cacagccuac gcuucacgau uuaucaagaa uuucguacag     2280

aaccaccucc aguuuagcca gaaugauacu auuaaacaaa agguuaaggu gcggucuggc     2340

gcccuaacca accagcugcg uuauaauugg gguaucgaag agaaaaacag agacaauaac     2400

cuccaucacg cagaagaugc cauaauucug gcuuucgcca cacaggggga gguccagagg     2460

auguccacua ucagugcaaa gcgcgaagac uucaaauacc aaaccgcgaa ggagcggaaa     2520

auuaaguuua caccaccguu cagcgauuuu aagucugugc uugacgaguc cgucggcgau     2580

aucuucgugu cauuugcucc cagaaggaaa auaaguggag ccgcacacaa ggccaccauc     2640

aaaccuaagg acacuggcgg gaaauauaag uucccaguua auaacggaau ggcugaaaau     2700

ggugugauuc agcgaguaga ugucuuuaaa aacgccaaga auaaguacca guucaucgug     2760

uuuuaccccg cagacuucua uaaagaugac uuuccugagu uggaucugaa gggcaacgaa     2820

auugacaaaa acguucaguu cuuauucagc cucuuuaagg acgaucugau cgaguucaaa     2880

acgaagaaga caaaaaccaa ggacucgaaa caucuucugg cuuacuuuaa guauauacgc     2940

uccgauggga aaauugccuu ccaacagaau uacaaggcgg agaucgaacg gaaggagucu     3000

gacaacaaaa agggauauaa agauaucuug cucgccacug gcagcggucu ggcuucacua     3060

aagaaauacc aggugagugu ccugggggac auugcagaag ugaagaauga gaagagacuc     3120

ccacugauca aacaaaugua uaaguccaaa cccaagaaaa aguga                     3165


<210>  2644
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2644
augaaccaga aguucauccu gggccucgac auugggauaa ccagcguggg auacggucuu       60

aucgauuaug agacaaagaa uauuaucgac gcaggcguca gacuguuucc cgaagcuaac      120

guugagaaua acgaagggag gcgguccaag cgcggaucuc gacguuugaa aagaaggcgg      180

auucaccgcu uagagagagu gaagaaacug cuagaagauu acaaucuccu ggaccaauca      240

cagaucccuc agaguacuaa cccauaugca auaaggguaa agggccuuag cgaggcccug      300

uccaaggaug aguuggugau cgcgcuccug cauauugcua aacggcgcgg uauccacaag      360

auugacguca ucgauucuaa ugacgaugug gggaacgaac ugucgacgaa agagcagcuc      420

aauaagaacu caaaauuacu gaaggacaag uucguuugcc aaauacagcu ugaacgaaug      480

aaugagggac aggugagagg cgagaaaaac agguuuaaga ccgccgauau uaucaaagaa      540

auuauccagu ugcugaaugu ccaaaagaac uuccaucagc uagacgagaa uuuuaucaac      600

aaauacauug aacucgugga gaugcggcgu gaauauuucg agggcccggg gaagggaagc      660

cccuacgguu gggagggcga uccuaaagca ugguaugaaa cacugauggg gcacuguacu      720

uacuuuccag acgagcuucg caguguaaag uacgccuauu ccgcugaccu guucaacgca      780

uugaaugauc ucaacaaucu gguuauacag agagacggau uaucuaagcu ggaauaccau      840

gagaaauauc acaucauuga aaacguguuu aagcagaaaa agaaacccac ccucaagcaa      900

aucgccaaug agauaaacgu caauccugag gauauuaagg gcuacaggau cacaaaaagc      960

gguaagccac aguucaccga auuuaaacuu uaucacgacc ugaaguccgu guuguucgau     1020

cagucaaucc uagagaacga agacgugcug gaucaaauug cugagauccu cacuauuuac     1080

caggacaaag auaguauaaa gagcaagcug acagaacuug acauccuguu gaaugaggag     1140

gauaaagaaa acauugccca gcucaccggg uauacgggaa cucaucggcu gucuuuaaag     1200

ugcauccgac ugguccuaga ggaacagugg uacuccucac gcaaucaaau ggagaucuuu     1260

acccaccuca acauuaaacc caagaaaaua aaucugacag cggcaaacaa gaucccuaag     1320

gccaugauug acgaguucau ccuuagccca guugugaaaa gaacuuucgg ccaggcuauu     1380

aauuugauca acaagauaau cgaaaaauac ggcguaccgg aggacauuau cauugaacug     1440

gccagggaga acaauaguaa ggauaaacag aaguuuauca acgaaaugca gaaaaagaau     1500

gagaacaccc ggaagcgcau aaaugagauu aucgggaaau auggaaacca aaaugcaaag     1560

agacucgugg aaaagauuag gcugcaugac gagcaggaag guaagugucu uuacucucug     1620

gaguccaucc ccuuggaaga ucuccugaac aauccuaacc acuaugaggu cgaccacaua     1680

aucccacgua gcgugucguu cgauaauucu uaccauaaca aaguuuuagu gaagcagucc     1740

gaaaauucaa agaaaaguaa ccugacaccc uaucaguacu uuaauagcgg caaguccaaa     1800

cuaucuuaua accaauucaa gcagcacauu cucaaccuga gcaaaucaca ggaccggauc     1860

aguaagaaga aaaaggaaua ccuuuuggag gaacgagaua uuaauaaauu ugagguccag     1920

aaggaguuca ucaacagaaa ucugguggac accagguacg cuacgcgcga gcucacuaac     1980

uaucugaaag ccuacuuuuc cgcgaauaac augaauguaa agguuaagac aauaaacggg     2040

agcuucaccg auuauuuacg gaaagugugg aaguuuaaaa aggagagaaa ucauggauac     2100

aaacaccaug ccgaagacgc acugauuauc gcuaacgccg auuuccuuuu uaaggagaau     2160

aaaaaguuga aggcugucaa cucugugcuc gaaaaaccug agaucgaguc aaagcaacug     2220

gacauucagg ucgauaguga agacaauuau agcgagaugu ucaucauucc aaaacaggug     2280

caagacauaa aggauuuuag gaacuucaaa uacucccacc gcguugacaa gaagcccaau     2340

cgacagcuaa ucaacgauac ucuguauucu acacggaaaa aggacaacuc gaccuacauu     2400

gugcagacga ucaaagauau uuacgccaag gacaauacca cacucaaaaa gcaguucgau     2460

aagagcccgg aaaaauuucu gauguaucaa cacgacccuc guacuuucga gaaacuugaa     2520

guaaucauga aacaguacgc aaacgagaag aaucccuugg ccaaauauca ugaggaaacc     2580

ggcgaguacc ugacaaagua uuccaagaaa aacaaugguc caauagucaa gucauuaaaa     2640

uacaucggga acaagcucgg cagucaccug gaugugacuc aucaguuuaa aucuagcacc     2700

aagaaacuug uuaaacuguc cauuaagccu uauagauucg acguguacuu gacagauaaa     2760

ggauacaagu uuaucaccau uucauaucuc gacguccuga aaaaggauaa uuacuauuac     2820

auccccgaac agaaauauga caagcuaaag cugggcaaag cuauagacaa gaacgcgaaa     2880

uucauugcca gcuuuuacaa gaaugaucuc aucaaacugg acggggagau cuauaagauu     2940

auaggaguga acucugauac uaggaauaug aucgaacuug acuugccaga uauucgcuac     3000

aaggaguacu gcgagcugaa caauaucaaa ggugaacccc ggauuaagaa aacgaucggc     3060

aagaaaguaa acuccauaga gaaguuaaca accgacgugc ucgggaaugu uuucacuaac     3120

acccaauaua caaagccuca gcugcuuuuu aaaagaggaa auuga                     3165


<210>  2645
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2645
augaagcuga caccccucaa cuacauccuu ggccuggacu uggggauugc cagcguggga       60

ugggcugucg uugagauaga ugaacaggag aauccuuuag gucugaucga cgugggcgua      120

agaaccuucg auagggcaga agugccaaaa acuggggagu cccuagcccu cgcgcggcgc      180

cuggcucgau cugcccguag acuugucaag aggcgggcag accgcauuaa aaaggccaaa      240

agacuguuga aggcugaaaa cauccuccug ucagcagaug agcaccuccc gaaugacgug      300

uggcaacuga ggguuaaggg auuagaucag aaacuggagc ggcaggaaug ggccgcugug      360

cuuuugcauc ugcuaaagca ccgcggcuau cucagucagc gaaagaacga gagcaaaucc      420

gaaaauaaag agcugggugc ccuucugucu ggggucgaga cgaaccauca aauuuugcag      480

ucggcggaau acagaacccc cgcagagauc gccgugaaga aguuucacgu agaagacgga      540

cauauaagga aucagcgggg cgcuuauaca cacacuuucu cacgucucga ucuguuagcc      600

gagauggaac ugcucuuuca gcgccaaacc gaccuuggca acccucacac aagcgcaaaa      660

cuguuggaga aucuaaccgc ucugcucaug uggcagaagc cagcccuggc gggggaggcc      720

auccuuaaaa ugcugggaaa gugcacuuuc gaacccacag aguacaaagc agcuaagaac      780

aguuauuccg ccgaaagauu uguuugguug accaaacuca auaaccugag gauuuuagag      840

cagggugcug aacgggcccu gacggauaau gagcgauucg cacuacucga ccagccuuac      900

gagaaggcca aguuuacuua ugcucaagcg cgcaccaugc uggcacuucc agaugaagcc      960

aucuucaaag gcgugagaua ccagggggag gacaagaaag cugucgaaac aaagacuauu     1020

uugauggaga ugaaagccua ccaucagauc aggaaggcac uggaaaacgc cgaccucaag     1080

gcugagugga augagcugaa aaacaacucu gaacuucugg augacauagg aaccgccuuu     1140

agcuugcaca agacagauga ggacauuugu cgguaucucg auggcaaacu guccgaauca     1200

aucuuaaaug cacugcuaga gaaccucaau uucgacaagu uuauucaacu gagucuuaaa     1260

gcguugcagc agauccugcc ccucaugcug cagggucaac gcuacgauga ggcugugagc     1320

gccaucuaug gggaccauua cggaaagaag ucugcagaaa uuaacagauu acugccuacc     1380

auaccagccg augagaucag gaauccggug guccuucgua cguugacuca ggcucggaaa     1440

guuauuaacg ccgugguacg acucuauggc ucccccgcaa gaauccacau agaaacaggc     1500

agggaggugg ggaagucaua ccaggaccgc aaaaagcugg aaaaacagca agaggauaau     1560

cggaagcaga gagagagcgc ugucaagaaa uucaaggaau auuuuccuaa cuucguggga     1620

gagccaaaag guaaggacau ucuaaaaaug aggcuguacg aacuccagca ggccaagugc     1680

cuguacagug gcaaaucucu ugaguugcac cgccuguuag aaaaggggua uguugaggug     1740

gaccaugcgc uccccuucuc ccgaaccugg gaugacagcu uuaauaacaa gguccuggug     1800

cuugccaaug agaaccaaaa uaaaggaaac cugacuccuu acgaaugguu ggauggcaag     1860

aauaacucgg agcacuggca gaacuucgua gcucggguuc agacaucugg uuuuucccau     1920

accaaaaagc agcguauccu cucacacaaa cuggacgaaa agggguucau cgagagaaau     1980

cuaaacgaua cgagguaugu ggcacgcuuu cuguguaauu ucauugccga caacaugcuc     2040

cugaccggca agggaaaacg gaaggucuuu gcuaguaaug gccaaaucac agcccuuuug     2100

agagggaggu ggggacugca gaaagugcga gaugacaacg aucgccauca cgcauuagac     2160

gcggucgugg uugccugcag cacuguggua augcagcaaa agauuacccg guucgucaga     2220

uacgaagagg guaauguguu uuccggcgag aggauagauc gugaaacagg ggagaucauu     2280

ccacuccacu uccccucucc gugggcuuuc uuccgcgaaa acguugagau ccggaucuuu     2340

agcgagaauc cuaaacugga acuugagaac agacugcccg acuauccaca guacaaucau     2400

gaauuugugc agccucuauu cgucucaagg augcccacuc gaaagaugac cggacagggc     2460

cacauggaga cagugaagag ugccaaacgg cucgaugaag gucuguccgu auugaaggug     2520

ccacugaccc aauuaaaacu cagcgaccug gagcgcaugg uuaacagaga gagggaaguc     2580

acucuuuaug agucucugaa ggcacgguug gaacaguuug ggaaugaccc cgcuaaagcc     2640

uucgcagagc cuuuucauaa gaagggcgga gccgugguga aagcuguccg aguugaacag     2700

acgcagaagu caggcgugcu cgugagagau gggaacggag uagcggacaa ugccaguaug     2760

guccgcgugg auguuuucac aaaagguggc aaguacuuuc uggugccaau uuacaccugg     2820

caagucgcaa aagggauacu accgaacaag gcugugacug ccaacguuga cgagaucgau     2880

uggcuggaga uggacgaaag cuaucaguuc auuuuuacca uguaccccaa ugaucuugug     2940

aaaguaaagc ucaagaaaga ggaguucuuu ggauauuacg gcggucugga cagggccaca     3000

ggggcuaucg ucauuaagga acacgauuug gaaaaaucca agggcaaaca gggaaucuau     3060

cguauaggcg ugaagcuggc auuaucuuuc gagaaguacc agguggacga gcucgggaaa     3120

aacauccggc cuuguagacc aaccaagagg caacacgucc gcuga                     3165


<210>  2646
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2646
augaaggaga aauacauccu gggccucgac cuugggauua ccagcguggg auaugguaua       60

aucaacuucg aaacaaagaa aauuaucgau gccggcguca gacuguuucc cgaggccaau      120

guugacaaca augaagggag gcgguccaag cgcggaucuc gacguuugaa aagaaggcgg      180

auucaccgcu uagagagagu gaagcugcua cucacugaau acgaucugau caacaaggag      240

cagauaccua cgucaaauaa cccauaucaa aucaggguaa aaggccuuag ugagauucug      300

agcaaggacg aauuggcaau cgcccuccug caucuggcga aacggcgcgg uauucacaau      360

aucaacgugu ccucugagga ugaagacgcu ucgaaugagc ucucaaccaa ggagcagaua      420

aaccgaaaua acaaauuacu gaaggauaag uacgucugcg aagugcagcu ucagagauug      480

aaagaggggc aaauuagggg agaaaagaau cgguucaaaa caacugacau ccugaaggag      540

auugaucagc uacucaaagu ucagaaggac uaucauaacc uggauaucga cuuuaucaau      600

caguacaaag aaauugugga gacccgucgc gaguauuucg aaggcccggg ccaagggagc      660

cccuuuggau ggaacgguga ucuuaagaag ugguacgaga ugcugauggg ccacuguaca      720

uacuucccuc aggaauugag aagugucaaa uaugccuacu ccgcagaccu cuuuaacgcc      780

cugaaugacu uaaacaaucu gauaauccag agggauaacu cugagaagcu cgaauaucau      840

gagaaauacc acauuaucga gaauguguuc aagcagaaaa agaagccaac ccuuaaacaa      900

auagcuaagg aaauuggggu aaaccccgag gacaucaaag gauaucggau cacuaagagc      960

ggcacaccuc aguuuaccga guucaaacug uaccacgauu ugaaguccau uguuuuugac     1020

aagucaaucc uagagaauga agcaauucug gaucagauag ccgagauccu cacgauuuau     1080

caagacgagc agaguaucaa agaagaacug aacaaacuuc cagaaauccu gaaugagcag     1140

gauaaggcug agauugccaa guugauaggu uacaacggga cucaucgacu cagccugaaa     1200

ugcauccacu uaauuaauga agaacugugg cagaccucuc gcaaccaaau ggaaaucuuc     1260

aauuaccuaa acauuaagcc caauaaggug gaccucuccg agcagaacaa aaucccuaag     1320

gauaugguca acgacuuuau acugucacca guggugaaaa gaacauucau ccagagcauu     1380

aaugucauca acaagguuau ugaaaaauau ggaaucccgg aggauauaau uaucgagcuu     1440

gcgagggaaa auaacaguga cgaccggaag aaauucauua auaacuugca gaagaagaau     1500

gaggcaacuc gcaaaagaau caacgaaaua aucggccaaa ccggcaauca gaacgccaag     1560

aggauugugg agaaaauccg ucugcaugau cagcaggaag ggaagugucu cuacucucug     1620

gaguccauug cucuuaugga ccuguugaau aacccccaaa auuaugaggu agaucacauc     1680

auaccucgga gcguggccuu ugacaacucg auucacaaua aaguccucgu gaagcagauc     1740

gaaaacucua agaaaggaaa ccgaacacca uaccaguauc uaaauuccuc agaugcaaag     1800

uuaaguuaca accaguucaa acaacauauc cugaaucuaa gcaaguccaa agacagaauu     1860

ucuaagaaga aaaaggauua ucuccuggag gaaagggaca ucaacaaauu ugagguucag     1920

aaggaguuca uuaaucgcaa ccuuguggau acccgguacg cuacgagaga guugacuagc     1980

uaccugaaag ccuauuuuuc agcgaauaac auggacguca aggugaagac aauaaauggu     2040

aguuucacca accaccucag gaaaguaugg cgcuuugaua aguaccgaaa ucauggcuau     2100

aaacaccaug ccgaggacgc acugaucauu gcuaacgccg auuucuuauu uaaggaaaau     2160

aaaaagcugc agaacacuaa uaaaauccuu gagaagccca caauugaaaa caacaccaag     2220

aaaguuacgg uggagaagga ggaagacuac aauaacgucu ucgagacccc uaaauuggug     2280

gaagacauca agcaauaucg ggauuacaaa uuuucccacc gugucgacaa gaagccaaau     2340

agacagcuca uaaacgauac acuguauagc acuaggauga aagacgagca cgauuacauc     2400

gugcagacca uuacagacau cuacgggaag gauaauacua accugaaaaa gcaguucaau     2460

aaaaaccccg aaaaguuucu gauguaucaa aaugacccga agaccuucga gaaacucucu     2520

auuaucauga agcaguacuc agaugagaaa aacccucugg cuaaguauua cgaagagaca     2580

ggagaauauc uuaccaaaua caguaagaag aauaacggcc ccauaguuaa aaagauuaaa     2640

uugcugggua auaagguggg gaaccauuua gacguaacua auaaauauga gaacagcacg     2700

aagaagcucg ucaaacuguc caucaagaau uaccgcuucg auguguaccu uacagaaaaa     2760

ggcuauaagu uuguuaccau cgccuaccug aacguguuca aaaaggacaa cuauuacuau     2820

auuccaaaag auaaguacca ggaguugaag gagaaaaaga aaauaaagga cacugaccag     2880

uuuaucgcau cuuucuauaa aaaugaucuc auuaagcuga acggagaccu auacaagauc     2940

auuggcguca auucggauga ccggaacauc auagaacugg auuacuauga caucaaauac     3000

aaggauuauu gcgagauuaa uaacaucaaa ggggaaccua gaauuaagaa aaccaucgga     3060

aagaagacag agagcauaga aaaauuuacc acugacgugc ucgguaaucu guaccuucac     3120

uccacagaga aggcucccca auugauuuuc aaaaggggcc uguga                     3165


<210>  2647
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2647
auggagaaca agaaucugaa cuacauccuc ggccuugacc uggggauugc cagcguggga       60

ugggcugucg uugaaauaga ugagaaagaa aaucccuuga gauuaaucga cgugggugua      120

aggaccuucg agcgggcaga agugccuaag acaggcgagu cccuggcccu aucucgccga      180

cucgcgcguu cagcuagaag gcugacucag cggcgcgucg ccagacuuaa aaaggcaaaa      240

aggcuguuga agagugagaa cauucuccug agcacggaug aacggcugcc acaccaagug      300

uggcagcucc gcguugaggg guuagaccau aagcuggaac gacaggagug ggccgcugug      360

cuuuugcacc ugaucaaaca uagaggauau cuaucccaga ggaagaauga gucuaaaucg      420

gaaaacaagg agcucggcgc acugcuuuca ggugucgaua auaaccacaa acuguugcaa      480

caggccaccu accggagccc ggcugaacuc gccgugaaga aguuugaggu agaagagggg      540

cauauucgua aucagcaggg agcguauaca cacacuuuca gucgccugga cuuacuggca      600

gagauggaac uccuuuucuc cagacaacag cacuucggca accccuuugc cucugagaaa      660

cuguuggaaa aucuaaccgc ucugcucaug uggcagaagc cugcccugag cggcgaggca      720

auccuuaaaa ugcuggggaa gugcacauuc gaagaugagu acaaagcugc caagaacacc      780

uauuccgcgg agagguuugu uuggauaacu aaauugaaua accuccggau ccaggaaaau      840

ggacuggagc gagccuuaaa cgacaacgaa cgccuggcac uaauggagca accauacgau      900

aagaauagac ucuucuauuc acaggugagg aguauucuga aguugagcga cgaagcuauc      960

uuuaaagguu ugcgguacuc uggcgaggau aagaaagcca uugagacaaa ggcuguccug     1020

auggaaauga aagccuacca ucagauccgc aaggugcucg aggggaacaa ucugaaggca     1080

gaaugggccg agcuuaaagc uaaccccacc cuguuggacg agauaggaac ggcguucucc     1140

cucuauaaga cugaugaaga cauuucagca uaccuggccg gcaaauuaag ccaaccugug     1200

cugaaugcuc uacucgagaa ccugaguuuu gacaaguuca uccagcuuuc uuugaaagcc     1260

cuguauaagc uccugccauu aaugcagcag ggucugagau acgaugaagc auguagggag     1320

auuuaugggg accacuacgg aaagaaaacc gaagagaauc aucacuuucu uccccaaauc     1380

ccugccgaug agauccguaa cccagucguu uugcggacac ucacucaggc ucgaaaggug     1440

auuaauggcg uagugagacu guauggcucc ccggccagga uacacaucga aaccgggcgc     1500

gaggucggaa aaagcuacaa ggaccggaga gaacuagaga aaaggcagga agagaaccgc     1560

aagcagcgag agaaugcaau uaaggaauuu aaagaguacu ucccccauuu ugcgggugaa     1620

ccuaaggcua aagauauccu gaagaugcgg cucuauaaac aacagaacgc caagugccug     1680

uacucgggca aaccaauaga gcuucaccgu uugcuggaaa agggguaugu ggagguugac     1740

caugcauuac ccuucucuag aacaugggau gacuccuuua auaacaaggu gcucguccug     1800

gccaaugaga accagaacaa aggaaaucuu accccuuucg aauggcugga uggcaagcac     1860

aacucagaga gguggcgcgc uuuuaaagcc uugguggaaa cgagugcauu cccauacgcu     1920

aagaaacagc ggauucucag ccaaaagcug gacgagaagg guuuuaucga gagaaaucua     1980

aacgauacua gguauguagc ccgauuccug uguaauuuua ucgcggacaa caugcaucuc     2040

acaggggaag gcaaacgcaa gguuuucgcc uccaauggac agauuaccgc ucugcuucgg     2100

ucuagauggg gcuuggcaaa aagcagggag gacaacgauc gucaccacgc ccuggacgcu     2160

guggucgugg ccugcucaac ugucgcaaug cagcagaaga ucacacgcuu ugugcgguuc     2220

gaagcggggg auguuuuuac cggagagaga auugacaggg aaacggguga gauaaucccc     2280

uuacauuucc cgaccccuug gcaauucuuu aaacaggagg uggaaauucg aaucuucagu     2340

gauaauccca agcucgagcu ggaaaaccgg cuuccagacc gcccucaggc caaucacgag     2400

uuuguacaac cccuguucgu cuccagaaug ccaacaagga agaugacugg ccaggggcau     2460

auggaaaccg ugaaaagcgc uaagcggcua aacgagggaa ucucuguuau uaaaaugccc     2520

cucacaaagc ugaaauugaa ggaucuggag uuaaugguga aucgagaaag agagaaggac     2580

cucuacgaua cucugaaagc ccgccuugaa gcauuuaacg acgauccugc uaaggccuuc     2640

gcagagccau uuauuaaaaa gggcggugcc aucgucaaau cagugagggu agaacagauu     2700

cagaagagug gggugcuggu ucgugagggc aauggagucg cugacaacgc gagcauggug     2760

cggguggaug ucuucaccaa aggcgggaag uauuuucuug uuccgaucua cacauggcaa     2820

guggccaagg gaauacuccc caacaaagca gcuacccagu acaaggacga ggaagacugg     2880

gaggugaugg auaauuccgc cacuuucaaa uuuucucugc acccuaacga ccuaguaaag     2940

cuggucacga aaaagaagac aauccuuggu uauuucaaug gccucaacag agccaccggg     3000

aauauagaua ucaaagaaca cgaccuggau aagucgaaag gaaagcaggg cauuuuugag     3060

ggugugggga ucaaauuggc ucugagcuuc gagaaguacc agguugacga auuaggcaag     3120

aacauuaggc ucuguaaacc auccaagcgc caacccgugc gauga                     3165


<210>  2648
<211>  3168
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2648
augaacaauu acauccuggg ccucgacauu gggauaacca gcgugggaua ugguaucguc       60

gauuccgaca caagagagau uaaggaugcc ggcguuaggc uuuuccccga agcuaacgug      120

gacaauaacg aggggcggcg cucuaaacga ggagcacgua gacugaagag gcggcgcauc      180

cacagauugg auaggguaaa acauuuacug gccgaauaca aucuacucga ccugacuaac      240

auuccuaagu caacgaaucc auaucagauc cgggugaaag gccuuaacga gaagcugagu      300

aaggaugaau uggucauagc gcuccugcac aucgcuaaac gccgagguau ucauaaugug      360

aacguuauga uggacgauaa ugacagcggg aacgagcugu ccaccaagga ucaacucaaa      420

aagaaugcca aagcauuauc ugacaaguac gugugcgagc ugcagcuuga aagauuugag      480

caggauuaua aggucagggg agagaaaaac cgguucaaga cagaggacuu ugugcgugag      540

gcccgcaaau ugcuggaaac ucagucgaag uucuuugaga ucgaccaaac cuucauuaug      600

agauacaucg aucuaguaga aacaaggcgg gaguauuuug aaggcccggg caaaggguca      660

cccuucggau gggaggguaa uauaaagaaa ugguuugagc agaugauggg ccacuguacc      720

uacuucccug aagaacuccg aagcguuaag uacgcuuaua gugcagaacu guuuaacgcc      780

cuuaacgacc ugaauaacuu ggugauuacu cgcgaugagg aagcuaagcu caauuacggg      840

gagaaauucc agaucauuga gaacgucuuu aagcagaaaa agacaccaaa ucugaaacaa      900

aucgccaagg aaaucggagu guccgagacc gacauuaagg gcuauagagu gaacaaaucu      960

gguaagcccg aguucacgca guucaaauua uaccaugauc ugaagaauau auuugaagac     1020

agcaaauauc ucaacgaugu ccagcuuaug gacaauaucg cggaaauuau cacuauauac     1080

caggauccug aguccauuau caaggagcug aacuugcuac cagaacugcu cucagagaag     1140

gaaaaagaga agaucagugc acugagcggg uaugccggaa cccacaggcu uucucugaaa     1200

ugcauuaauu ugcuccugga cgauuuaugg gaguccucac ugaaccaaau ggaacuauuc     1260

acaaagcuca aucugaaacc caagaagauc gaccuuagcc agcagcacaa aauuccuaua     1320

aaguugguug acgauuuuau ccugagucca gugguaaaac gggcuuucau ucaaucuauc     1380

caggugguca acgccaucau ugacaaguac ggccucccgg aggauauaau cauugaacug     1440

gcacgcgaga auaacuccga cgauagaagg aaauuucuua accagcugca gaagcaaaau     1500

gcugaaacuc guaaacaggu ggagaagguu uugcgggagu acggcaacga caaugccaag     1560

cgaaucgugc agaaaauuaa gcuccauaac augcaggaag ggaaaugucu guauagcuua     1620

aaggauaucc cccuggagga ccuacucaaa aauccuaacc acuacgaagu cgaucauaua     1680

aucccaagau cgguggcguu cgacaauucu augcacaaca agguacuggu uagggccgag     1740

gaaaauucca agaaaggaaa ccgcaccccc uaucaauacc uuaauucaag ugagagcucc     1800

uugucuuaua acgaguuuaa gcagcacauu cugaaucuca gcaaaacaaa ggaucggauc     1860

accaaaaaga agagagaaua ccuguuagag gaaagggaca uuaacaaaua ugaugugcag     1920

aaggaguuca ucaaucgcaa ccuggucgac acgcgauacg caacucggga acuuacauca     1980

uugcucaagg cuuacuuuag ugccaacaau cuggacguga aagucaaaac cauaaacggu     2040

uccuucacua auuaucuacg uaaggugugg aaguuugaua aagacagaaa caagggcuac     2100

aaacaucacg cugaggaugc ccugauuauc gcaaaugccg acuuccucuu uaagcauaac     2160

aaaaagcuga ggaauauuaa caaaguucuu gaugcuccua gcaaggagau cgacaagaaa     2220

cgcgugacag uacagucuga agaugaguau aaucaaaugu ucgaagacac ccagaaggcg     2280

caggcaauca aaaaguucga gauacggaaa uuuucacaca gagucgauaa gaagccaaac     2340

aggcaguuga uuaaagacac gcuguacagu acccgaaaua ucgaugggau ugaguaugug     2400

guugaaagca ucaaggacau auacuccgug aacaaugaua aagucaagac aaaauucaag     2460

aaggaccccc aucgcuuacu cauguaucgg aacgacccgc aaacuuuuga gaaauucgaa     2520

aagguguuua aacaguacga gucugaaaag aauccuuucg ccaaauacua ugaggagacc     2580

ggagaaaaga uuagaaaguu uucgaaaaca ggccaggguc ccuacaucaa caagaucaaa     2640

uaucugaggg agcgucuugg gcgccacugc gauguaacua acaaguacau uaauagccgg     2700

aacaaaaucg ugcaacugaa gauuuauucc uacagauucg acauauauca guacggcaau     2760

aacuacaaga ugaucaccau uucauauauc gauuuggaac agaaaaguaa uuacuauuac     2820

auuucuaggg agaaguauga acagaaaaag aaagacaagc aaaucgauga cagcuacaaa     2880

uuuauaggau ccuucuauaa gaacgauauc auuaauuaca acggcgagau guaccgaguu     2940

aucgggguca augacucaga gaagaacaaa auucagcucg auaugaucga cauaagcauu     3000

aaggauuaua uggaacugaa uaacaucaaa aagacaggag ugaucuacaa aaccauuggu     3060

aagucuacua cgcacauaga gaaguauaca accgacaucc uaggcaaucu guacaaagcu     3120

gccccaccua agaaacccca gcucauuuuu aagcggggga uggaauga                  3168


<210>  2649
<211>  3168
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2649
augaccccca ucagcgaguc ccuacucuuc ggccuugacg uggggauuuc uucaugcgga       60

ugggccgucc ugaagcacag ugauacaacu ggucauauag aggcuuuggg cagcuggugu      120

uuuaacgcac cugaaacgaa aaccgaaaga acaccaacua aucagaucag gcgguccucu      180

cgcuuacugc gacguguugu gagaaggcgg cgcaacagaa ugaccgagau uaggcggcua      240

uucgcccgcc acgggcucau cgacaaugau cgaccggacg cgcugagaca acccgcuauu      300

gauccuuggg aaaugagggg acagggccuu gaccggcagc ugacaccaga ggaguuugcc      360

guagcauugg cccauaucgc uaagcaccgu gguuucaaau cgucagcaaa gcgcgugaaa      420

agcaacgcca gucccgauga cuccaagaug cucgcugccc uggaaaagac caaagagcug      480

ucugcgcagu acagaacuau aggggaaaug uuugcaaggg auagcgccua uguccaacgg      540

cgacgcaaua gagacggacu cuucgauagg acaaucuccc gggacgauuu aauugacgag      600

gcuaagaucc uguuugaccg ccagagaaaa cuuggcaacc cuguggccuc aaccgagauu      660

gaagaggcau ucacgaggau cgcuuuuugg cagaauccaa ugcaggauag uggcguuuug      720

guggccagcu gccccuucga accuaaggag aaacguucuu cccgguuuuc accgagcuuc      780

gaacgauuua gacugcuaug uaggcucaua aaccugcgcg ucacugacgg gccagaggag      840

cggccccuua ccgaagagga acugagauug gcguacgagc augccggaaa gacaaggaag      900

cucacugcag cugccauucg caaacugauc gguuuacgag augaccaaca cuucaccaca      960

auuccuccag aacaggagaa gcaggauauc accagucgga cgggcgagac uuucgcuggg     1020

acagccaccu uucguucugu gcugggagaa ucccucugga gacagcuucu gccccacccu     1080

gagugguugg acaaugcagc cuggaucauu agcuuccaug aacuaacuga gacaauaauc     1140

aaaaagcugg gcgaaauugg ucucccagua gaggcuuaug agcugaucug cgaugcgcuu     1200

aaaucggaca agucucccuu ugcaaaauuc aagggggccu ccucacugag ugauaaggcu     1260

guuagggccu ugauaccgua ccuccaacag ggacugaccu augacaaagc augucagaag     1320

gccggcuacg aucacgcugc cagccgcuuu uccggccggg caacgauuga caccaaagcg     1380

aaguucaacg cuuuaauggc cgaagugaga gacucuaucg caaauccuau cgccaggaaa     1440

gcucugagcg aggggaugaa gcaacuaugg gccaugcgaa accgcugggg acuccccggu     1500

gcaauuugca ucgaacuggc ucgggauguc ggcaauucac uugagaagag aaaagagauu     1560

gaaaggaacu ugaagacaac ugccaaacag cgugcgcgcg agcgggaaga ggccugugaa     1620

cugcucaauc ugaaggaggu gaguuccgac auacuucuga gauauagguu guggaaagag     1680

caagggggac gaugcuguua cagcggcaag ucuaucaccc cagauaugcu cauggcucag     1740

gacaaccgga uucagaucga ucauauccug ccuuggucac gcuuugguga cgauaguuac     1800

agcaacaagg gguuaugccu ggcaaaagaa aaucaggcca agaaaaacca gacacccuau     1860

gaguggauuc acggccaggg aacuaccgcu caaaaggaac agugggacaa auucguggcc     1920

gaugucgagu ccagaaagaa uacaaagggc cugaaaaaga ggaacuuucu ccugaaaggg     1980

ucuaaggaaa uagaggagcg guuccgaucg agaaaucuua acgacacccg cuacgcagcg     2040

agguugaugg uugaagccgc ucgucugcuc uauccagccg gacagcgggg ugagaaaggc     2100

gggacuagaa ggguguuuac gcgccccgga gcacugacag cugccuuacg acacgcaugg     2160

ggcguagaaa gccugaagaa aaucgauggu gagagacuuc augacaaucg gcaccaugcc     2220

uuggaugcug cgauuguggc cgcagucucc gaagcugaga uccagaagcu caccaaguca     2280

uuccaagagu gugaacagaa agggcugagu aggccuaugc gcgacguggc cccaccgugg     2340

gacggcuuuc gugaucagcu aaaggagaaa uacgaaacua uuuucguugc cagacccgag     2400

cggcagaggg cucgcggaca aggccacgag gcaaccaucc gacaggugag agaagaggaa     2460

acagggccug ucauauauga gcggaaggcc gugucugcgc ugacccucgc ugaccuggcc     2520

aaaaucaagg auccagacag gaacgcagga cuuauugcca gcuugcgcca guggaucgaa     2580

gcugguaagc ccgcagauaa accuccaaga acucccugcc aucaauccgc cgcgggccac     2640

gagauuagga agguacggcu ggcuacaauc auaacgccgg ccguuccugu gcguggggga     2700

auggcaaaac gcggcgagau gacccgaguc gacauuuuca aggugaucgg uaaaaagggg     2760

aagccagaau gguacaugau ucccaucuau ccucaccaga uaaugaauag aucacagugg     2820

cccgccccac cuaacaaagc ugucguggcc ggcaagagcg aggcagauug gcccaucaug     2880

ggagcugaag cccaguuuca auucucuuua uacccaugcu ccuacguuag gcucuuuaaa     2940

gagggcacug ugauugaagg guauuucugu ggaugccauc ggagucgcgc guacaucaca     3000

cuggcccuua gcaccggcgc agcuccgcug cagaagggua uugggaucaa aacacaggag     3060

cacauacaga aguuuucugu aaauagauuc ggagaguugu ccccugucaa gcaagaaacu     3120

aggaccuggc auggcguggc cuguacguca cccgaccagc acgauuga                  3168


<210>  2650
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2650
augaaggccc ugaacuacau ccucggccuu gaccugggga uugcuagcgu gggaugggca       60

gucguugaga uagaucagaa ugaauugccc aucagauuaa uugaccuggg ugugaggacc      120

uucgagcggg ccgaaguacc uaaaacaggc gauucccuag cgcucgcucg ccgacuggcc      180

cguucuucaa gaaggcuugu gcggcgcaga gcagucaggc uguugaaggc caaacggcuc      240

cugaagcaag aggggauccu gcaggcugac gauuuucaca guagccaucu caaaccauua      300

ccgaacaauc ccugggaacu gcgcgugaag ggacuuaacc acuugcugga ccgaaaggag      360

ugggcagccg uucuacucca ucugcuuaaa cacagaggcu aucuguccca gaggaagaau      420

gagucuaaaa cugauaacaa ggaauugggu gcucuccuga aagggguguc ggagaaucgg      480

caguuacugc aagcccaggc guucucaacg ccugcagaaa uugccaucaa gaaguuucag      540

caggaggagg gacauauacg uaaccaacgc ggcgcuuaca gccacaccuu cgacagaaau      600

gaucuccuug ccgaacugag guugcuauuu cagaaacagc gggaguucaa caauccauau      660

acaagucagu cccuggaaca agcaaucgcu gagcuccuga uguggcagaa gcccgcccuu      720

ucuggcgaag cgauucugaa aauguugggg aagugcacuu uugagccuag cgaguacaaa      780

gccccaaaga acuccuauuc agcagaauac uucgucuggc ucaccaaacu gaauaacuua      840

cgaauccugg agaauggagc ugaccgcggu cuaacagccg aagagagaca gcuccugauu      900

aaccaacccu uugaaaaggc uaaguucacc uaugcccagg caaggaaacu uuuggagcug      960

agugaucucg ccaucuuuaa gggcgugcgg uacgggaaag acaacgcuga gagcgcgacu     1020

cugauggaaa ugaaggcaug gcacgccaua cgcaaagcuc uugagucuaa uggacugaag     1080

acagaaugga acggcuugaa gaccaaaccu gagcuccugg auaagauugg uacggccuuc     1140

uccaucuaca aaacugacga agacauuuca caguauuuaa auggggagcu gagcccagca     1200

guacuaaacg cccuccugga gaaucuuagu uuugauaagu ucaucagauu gucucugcag     1260

ucccucgcua aaauccugcc guuaauggaa caaggacuga gguacgacga ggccuguaag     1320

cagguuuaug gcgaucauua cggcaagaaa gaacagcuug aguugcguuu ccuccccaag     1380

auuccugugg aggacauacg gaacccaguc gugcugcgaa cccuaagcca ggcaagaaaa     1440

gugaucaaug gggucguuag gcuguaugga ucgcccgcgc gcauucacau cgaaacaggu     1500

cgggaggugg gcaagucuuu uuccgaaaga agggauauau caaaacgcca agaggaaaac     1560

cgaaaugagc ggcagcgugc uguagagaag uucaaggaau uuuucccuca uuuugugggg     1620

gagccaaaag ccaaggacau ucucaaaaug agacuguacg aacagcagca cggaaagugc     1680

cuuuauagug gcaaagaguu ggaucugaac agguuacucg aaaaggguua cgucgagauc     1740

gaccacgcac ugcccuucag ccgcacuugg gaugacuccu uuaauaacaa agugcuuguu     1800

cuggccucug agaaucaaaa caaggggaau cagaccccgu acgaaugguu gaacggcaag     1860

gagaacgcug aacgguggaa acucuuccag gcccugguga auggaagcag auuuccuuau     1920

ucaaagaaac agaggauccu aacaaaguac cuggaugagg acggcuuccg agaacgcaac     1980

cucaaugaca cccgguaugu cgcaagauuu cuguguaacu ucauugagga uaauaugcaa     2040

cuuguggggc aggguaaaaa gaggguauuu gcuaguaacg gacagaucac ggccuugcug     2100

cgucgcuccu ggggcauuca aaagguucgg gaggacaaug auagacauca cgcgguggac     2160

gccgucgugg ucgcuugcag cacuagggca uuacagaaaa agauaacaga uuacgugcga     2220

uauaaagaaa ugaacguuuu caccggggag aagaucgacc aggaaacugg agagauuauc     2280

aaauuccauu uucccgagcc auggaaguuc uuucggcagg aaguggagau ccgcguauuc     2340

ucugaaaccc cugccgagga ugucgugauu gcucuccccg acagaccaca agccaaucac     2400

gaauuuauac agccccuguu cguuucaagg gcaccuacac ggaagaugac gggccagggu     2460

cauauggaga ccaucaaaag ugcgaagcga cuugccgagg ggcugagcgu guccagaguc     2520

ccacuaacac agcucaaacu gaaggauuug gaaaacaugg ugaaucgcga gagggaaaaa     2580

gcucuguacg aggccuuaaa gguacgucuc gaaaaguuua acaaugaccc ggcaaaagcc     2640

uucgcugauc ccuuuuauaa gaaaggcgga caacagguga aggcaguucg ggucgaacag     2700

gugcagaaau cuggcgugcu ggucagacaa gagaacgggg uugccgacaa ugcuucgaug     2760

gugagggugg auguauucau uaagggaggu aaauacuuuc uugucccuau cuacacuugg     2820

caaguggcga agggcauucu gccaaaccgc gccgcaaccc aggggaagga ggaggaauau     2880

ugggacauca uggacgagac agcuauauuc caguuuagcu uguaccagaa cgaucucauc     2940

aaacugguua cuaagaaaaa gaccauuuuc ggauauuuca auggccuaaa ccgagccaca     3000

uccaauauca acauuaaaga acacgaccug gauaagucaa aggguaaaga cgggaucuac     3060

cuugaggugg gcgucaagcu cgcccugagu uuggagaaau aucaggugga ugaacuggga     3120

aagaauauac agcccuguag aucuaugaaa aagcggccuc caguuaggug a              3171


<210>  2651
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2651
augcccaagc agaaaagcac cagauacagg cuggcccucg accuuggcuc cacaucucug       60

gggugggcug uguugcgguu aaacgagcaa cacgaaccua augcaaucau ucgcgccgga      120

guccgaauau ucucaaacgg ucguaaucca aaggauggca guagccuggc gguuacuaga      180

agggcugccc gggcaaugcg cagaaggcgg gaccgccuac ucaaacgaaa ggccagaaug      240

augaacaaac ugguggagua uggguuuuuc ccggcugaug uagcagaaag gaagaaacuu      300

gagcggauca aucccuacga acugcgugcc aaaggauugg acgcuccucu cacgccaggc      360

gaguuugccc gcgcgcuguu ccauauuaac cagagaaggg guuuuaaguc caaucggaaa      420

accgauaaga aagagaacga cucuggggca cugaaggccg cuaucucgca ccuccgacag      480

caguuacaug aacgcaaggc cagaacagug ggagaguggc uguggcaaag gcuucaggca      540

ggcuugggca cucgggcucg cuauagagaa ucaagggucg cccaggcgaa ugggaaaacc      600

cguauugaaa agagcuacga ucuguaugug gaccggcaga ugaucgccga ggaguucgau      660

gcacuauggg cugcccaagc ugccuuuaac ccccagcagu ucagugagca ggcacgagga      720

gaacucagag acguucugcu uuuucaaagg gaucugcgcc cugugcggcc agguagaugc      780

acauugcucc ccgacgagga aagggccccu cuggcuuuac cauccgucca gcgcuuccga      840

auacugcagg aggcgaauca ccuccggcuu cugcauccgg auuugcguga ggaaccccua      900

ucucuggcac aaagagacgc cgugguagac gcucucgagc acaacagcaa aaggaccuuu      960

gaacagcugc gcaaacuucu gaaguugaau ggcacuguua aguucaaccu cgaggaugaa     1020

aaacggaagg agcugaaggg gaaugccaca uccgcaaccu uaucaaaaga cggacuguuu     1080

ggcgagaacu gguggaaccu agaugaagcc cuccaggacg cuaucgugug gcagcugguc     1140

agugagcaaa gcgaagagaa acuucagaau ugguugcagg ccaacacggg ugugaaugca     1200

acucaggcgg aaaccauugc uaacgccggg cugccugagg gauacggcuc ucucuccuau     1260

aaggcacugg ccagaaucau uccagcucuu aggggcacag agguggccaa ugggaucgau     1320

gucccccgaa cuuacgacca agcaguucag gcugccggau ucgcgcacca uucagaucug     1380

gacagcgguu ucgauuuuga acacagucau gacgaggugg aacgcauagg cgagcgggua     1440

auugccucua ccggggaggu gaagccuguc uucgcuuuua aacaguugcc auacuaugga     1500

aaggcacuca gaaggcacgu ggccuucgcu aaggaacacc cccguaacga ggaggaacgc     1560

uacggcaaaa ucgccaaucc gacaguucau auuggucuga accaguuacg gguggucgug     1620

aaugcacuga ucagaaggua ugggcgaccu accgaaguag uuguggagcu agcgcgggau     1680

cucaagcaau cccgcgagca gagacaggag gaacagaaaa ggcaagccga cuaccagaag     1740

cggaacgaac acauccgaag agagauugcu agcaugcaug gcugugccga ggaacgcguc     1800

ucgcacgcag acgugcagaa auggauacug ugggaggaac uuucuuuuga ugucgcugac     1860

aggcguugcc ccuauuccgg agugcaaauc ucagcccaga uguugcugag uaaucagguu     1920

gagauugaac auauccuccc auucagccag acgcuggaua acuccuuaaa uaaccggacu     1980

guggcaauga gacaagccaa uaggauaaag ggcaaccgca caccuuggca ggcucgagag     2040

gacuuugaaa gacaggggug gucuuacgau agcauucugc agcgggcgga gaggaugccc     2100

aaggccaaac gcuaucguuu cucaagagac ggauacgagc gguggcuuaa ggaagauaaa     2160

gacuuuuugg caagggcucu caacgauacc cgcuaccuga gucgaguagc caagacuuau     2220

auggagcuag ucuguccacu ggguacaaga gugauccccg gccaaaugac cgcccuccug     2280

cgggcuaaau ucgggcuuaa ugacguuuug ggacugaacg gcgaaaagaa uagggaugac     2340

caccgccacc augcagugga ugccugcguc aucgguguga cggaccaggg guuacuccag     2400

agauuugcgc aggcuuccgc cgacgcaagg ggcaacggac uggagaaacu uguagagaau     2460

augccugugc caugggccac cuaccgggau cacguuaagc gugcuauuca auguaucugg     2520

gucagccaua agccggacca cggcuaugaa gggcccauga uggaggaaac agcauacggg     2580

auucgcaaag augguucuau acgacagaag acuaaagccg acggcucaac cgggagagag     2640

aucacacacc ugauuaggau cacugaaccu acccagaacu ugcggcaugg aguggaugcg     2700

caaggccagc cacucgcuua uaaggguuac gugggaggca guaaugacug caucgagauu     2760

acacagaacg agcagggaaa augggaaggc aaugucauaa ccacuuucca agccuaugau     2820

aucguucgca gacuggggag caagcaggga cccaggcggc uacgacaccc uacgcuggca     2880

cagacacucg acguguacgg caagccaaag aagcugguga ugagacuuau gauugaugac     2940

uguguacgcu uggagcugga ugguaacaaa aagaccuuaa gggucgugaa aaucacuggg     3000

aauggacagg uuuuuauggc ccccgugcau gaagcuaacg ucgacaagcg uaauuccgau     3060

aagaccgacc cguucgccua cgugucuaaa uaugcaggcu cguuccaaaa ggcuaaagcc     3120

cggagaauua caaucagccc uauaggugag cuccacgacc caggcuuuug a              3171


<210>  2652
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2652
augagcgaca aguccaaaau ccugggcuuc gauauuggga uaaccucuau cggaugggcc       60

uacguggagg gugaagaacu cauugacugc ggcgucagaa ucuuuacaaa ggcugaaaac      120

cccaaaacua aggagucacu ugcacugccu aggcgggaag cccgcggggu ucgacguaga      180

uuggcgaggc ggaaaggacg cuuaaaugcu auuaagcagc ugaucuguaa ggaguucggc      240

cuaaacuauc aagauuacau agcccaggac ggugagcucc caaaagcaua ugccgggaag      300

cugaucaauc cguacgaacu uagaaguaaa gcucugaggg agaaguugga aaaagaggag      360

cucgcacggg ugauucugca caucgccaag caucgcggau auggcaacaa gcacgcuaaa      420

gaaacggaug acgccgagag cggcaagauu aaaaaggcga ucuccaauaa cgaaaagaua      480

cugaaugaga agggguacca gucugcagga gaauaucucu accaggaguu uuuccaaaaa      540

gagcgauuac aggaagagaa gaaggguaac aauaccacaa aagaauuuaa gaacguaaga      600

aauaaagccg gcgauuacga gcauugcauu gcucagacuc agaugcaaaa cgaacugagg      660

cuuaucuucg agaagcagcg ggaguuuggg uugaauuucu cggaagaguu uaaaaagaag      720

gugcuggaaa ccgccuucuu ucagcguccc cuaaaagacu ucucagauaa gauuggagac      780

uguacauuua ucgagggcgc aaaacgcgcu ccuaaggaua gccucagugc caucgaguuc      840

gucgcgcugg guagaauuau aaacacccuu aaaaaucugc agaacacuuc cggggaggug      900

uauucuaggg acaagaucaa cgagauuuug caaaucguuc ucgauaaggg agaaauaagc      960

uacaaaaagc ugcgggagau uuuagacauc gaauccucag ugcgauucaa agacaguaag     1020

cuggauuaua caaaagagcu caaggaggcc gaaaagacca aacuuuuuga gagcaagaau     1080

cugcagaaau ucaagaaagc auugggcucu uuugaaggca ucucccgcga gauucuagac     1140

ucaaucgcua aggaaauuau gauaacgaaa gauaaggaga agcuggccaa agagcucaag     1200

aaauucccac uggacgaaac ucagauccaa aaccuuagca aucugaguuu uucucagacc     1260

auuaacuugu cccucgaggc ucugagccag aucuuacccu ucaugagagg ggaaagggag     1320

gauaagugcc ugcgguacga cgaagccguc gagaaagcag gacuaaagga gguggccaag     1380

aauaaaucga aggaugacuu ucucccucca cuggaaaacu augagcccua ccuugcuaau     1440

ccuguaguug cgcgcgcauu gucugaauau agaaaagugc ugaacgcccu ccugaggaag     1500

uacgguaaaa ugcacaagau ccauauugag uucacacgug aagucggcaa guccaaagag     1560

gagcggaaua agauagaaaa agagcagaag gaaaacuuug cuaaaaauca agaggcccag     1620

aagcagugug agauccuugg gcugccaauu aacggagcaa auaucuugaa gaugaaacuc     1680

uggcgagaac agcaagaguc augcguguac aguggcaagg aaauugccau cgaugaccug     1740

aaaaacccgg cuauauuaca gguggaucac aucuaucccu acagcagauc cuucgacgac     1800

ucuuauaaua acaagguccu gguuuuuagc aaagagaacc agaauaagcu aaacaaaacu     1860

ccuuucgaag ccuuugguga gaauaaggau aagugggaga aaauucucuc acuggcaagu     1920

aaacuuccaa aacccaagag gcagcgcauc uacaacaugc aauucaaaga caaggaacag     1980

ggguucuuga cccggaauau uaacgauaca ggcuauaucg cgagacuggu gucccagugg     2040

accaagaauu gucucagcuu ugagaaacug ucugaagacg agguaucaau agcuggagaa     2100

aagggcagua aagugcacgu cgaggugauu agcggguccu uaacggccau gcugaggcau     2160

uacuggggac uuggugauaa gaaccgcgac aaucacuugc aucacgcagu ugaugccauc     2220

auuaucgcuu auucuaacga gaaagugaua aaggccuucu cggacuacaa gaaacaccga     2280

gaacagaaua aggcaaaauu uuacgcuaag gagaucgccg aaagcgagua uaaaaagcaa     2340

aaggucuucu uugaaccuac uaaccauuuc cggcagaaag ugcucgagaa gauugauucc     2400

aucuuuguau caaaaccacc ccguaagaga gcgaggggcg cccugcacga ggaaacauuc     2460

uacaguuuug agcgccuaug caaugacaaa gaauaugcua agaaggagaa aauggauuac     2520

gggggaaagg caggcauuca gaaagccauc gcucugggua agauacggca aguugggacc     2580

aaaauugugu cuaacggcac uauggucaga guggacaucu uccagcauaa gagcaaugga     2640

aaauuuuaug gcgucccgau cuacacaaug gauuucgccc ucggggugcu gccuaacaag     2700

gcaguugugu ccggaaagga caaacagggu guaauuaagg acuggcuuga gauggauuca     2760

aacuaugaau uuuguuucag cuuguucaaa gacgaucuga ucuuagucca gaagaaagag     2820

auggaaaagg cggagcucug cuacuacacc gacuuuaauu cuaacacggc cggcauuaag     2880

uucgaaaaac acaauaaccu guaugugaau cuuaacgaga augagaggaa gcuguuuacc     2940

uccaaagaag uugugaaagg gggaaagaag aacgucugga aggauaaaac acccaaggag     3000

auagaacaau uggagaaaaa ucgaaagauc auuggcgguu ucgugacuau cgaagaccuc     3060

aaaaccuuug agaaguacca gguaagucca cugggggagg ugcagaaggc ugauuucgcc     3120

ccucgccagg caauuacacu aaaaagcucu cccaagcauc ggaucaacaa auga           3174


<210>  2653
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2653
augagcaaca gagugcccua caggcuggcc cucgaccuug gcaccaauuc caucgggugg       60

ugccuguugg auuuaaacgg aaauggugac ccuuguggcg uccggcgcau ggggguucga      120

auuuucccag auggacguga cccgaagucu aaagagucac uggcugcaga uagaaggcua      180

gcccggagua ugcgcagaag gcgggaccgc uaucuccgaa gaaggagcgc gcugaugaac      240

gcucuugugg ccgcaggccu gaugcccgcc gaucgggcua cacguaaggc auuggaaucc      300

gaggacccuu gcgaacuccg cgccagagcu cuguuugagc cacugacugc cggucaggua      360

gggagggcgc uuuuccacuu aaaucaacgg cgaggauuua aaucuaaccg caaggcagcc      420

ggcgaagaga augaggcugg caaaauaaag gccgggaucu cggcacugaa ggcugaaauu      480

gaggccucag gagaaacgac ccuuggugcg ugguuggccg caagacauag gaaacgggag      540

acagugcgcg cuagacugga uggcgccggg gcuaaggcca gcuacgaguu cuaucccgaa      600

agggggcuag uccacgacga guuucaggca auccguacug cccaggcucu ccaucacccu      660

gcggugaccg cagaacagug ggaucggguu gagaguguga uuuuccgaca aagaccacug      720

aggcccgucc gcccuggcaa auguacacuu uuuccagacc aggaacgggc cccgugggcu      780

cugcccgugg cccaggaguu cagaaucuug accgagauag guaaccucaa gcuguacagg      840

ccuggggaag aaccacgcga auuauccgca gaucagcaug cccaacuguu ugcuaaacuc      900

ugccgaucug ccgaccuuac uuucgagaag auccggaagg aacugaaauu ggaccccgga      960

cuaaagguaa aucuggaggg cgagaaacgu gauagacuca agggcgacgc aguuacaagc     1020

gcgcuguccg cuaaaaagag guuugggccu aaauggaacg cccuuacccc agaugaccgc     1080

cgggauauug ugggaagacu gaucgaagca gaggacgaag ccgaguuggc ugguuggcuc     1140

gaaucagucc acggccugcc cagugagaau gccgcacaca cggcugccag cccgggguua     1200

ccugauggau auuguaggcu gucugagaag gcgcuaguga agcucgugcc ccugaugcga     1260

gaacagcgcc uucgguacga cgaggccguc uuggcucuga accuccauca cuccgauuuc     1320

agaccagaag gccugcuuga cgagcugccu uauuacggug agcccuugga aagguuuauu     1380

gcauucgggu caggcgagcc aagcgauccc ccugaacagc aucucggacg uuucccaaau     1440

ccgacuguuc acaucggccu gaacgaguua cgcaaacugg ugaaucggau aguagaaaga     1500

ugggggaagc ccgccgacau uguggucgag cuagcuaggg accucaaacg aagucgggau     1560

gagcgcgaca ccauccagag agaacaagcc cagaaccaga ggaagaacga ggaucgguuu     1620

gcaaaaauua aggaacuggg aaucgacccu cgaccagauu cuaugcuuag auugcgccug     1680

ugggaggaac uccccaaggu ggguguuggc cacgugugcg ucuacacagg ggagccuauc     1740

ggaauuaggc gucuguuaga gggcgacgug gauauagacc auauccugcc auucucccgg     1800

acucuuagcg auggugcgga caauaaaauu guauguguua gaagggccaa ccgcuuuaag     1860

cgagauagau cgcccuggga ggcuuucggg gugaccgccg ucgacggcua ugauugggca     1920

gcuaucgccc aacgggcaga ggaauugccg aaaaauaaga gguggcgcuu ucucccugac     1980

gccauggacc guuucgaggg agaaagaggc uuucuggauc ggcagcuagu ggagacaagg     2040

caccugucuc gcgucgcucg acaguaccuc gcguccauag uggaccugaa caaaguuaga     2100

guggccccag ggaagcucac cgcaauguug cgggcuaggu ggggacugaa uaagauuuua     2160

ucagauggua accucaaaaa ucgcagugac cauagacacc augccaucga ugccgcugua     2220

cugggcauga ucgaccagag gcuuguccaa cugguggcac aggccgcggc ucgggccgag     2280

gcaaacgaau uggagcgucu cuucgauggg cugcccgacc cuuuugaugg acuagccgcu     2340

acgagcuucg gcgaccaggu ucgcgaaaag auucgaggua ucacuguguc ccacagagca     2400

gagcacgggu ggcagggcag gaugcauaau gauacagccu auggacccgu cgaggcggug     2460

aacggcguac acaccguggu uacucggaaa ccacugucug ggcucacagc uaaggacuuu     2520

ccuaauauuc gcgauagagu ccuggccaaa gcacuugaag accauguggc cgcugaggcc     2580

gcagcuggaa ggagcuucgg cgacgccuug gguucauuua aguggggagg acaacccgug     2640

cggcgaguca gaaugcugga uguuuuaggc aacccaauac cgauccagga cccuaguggu     2700

accguguuca agguguacaa aggcgauucc aaugcguggu augaaauucu ccgcgacccc     2760

agggggcggu ggacgggaaa gaucguaagc accuucgagg ccuaccgucu gcacagagaa     2820

uggcuugcag agacaggcgg ggauucugga aggggcucag gugucuuucu ggacugggcu     2880

gccaaacgcg ugguugggga ggcuggaacu ccacuauucc ggcucuuuaa gggcgaucug     2940

uuggcacugg gugaagcccc cgaccgaaga aucgugaggg ucgugaaauu cagugagggc     3000

cagguugccu uagcucucca ccuggaagcg gggaaccuua aggcacgcga ugccgacaaa     3060

ggagauccuu uuaaguauca guacugcagc ccagacuccc ugcggaagca agcugccaga     3120

cccgcaggcg uggacuugcu cggguacgua caugauaaag gaccucugga guga           3174


<210>  2654
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2654
augagaaggc ugggccucga cauugggacc aacagcauug gauggugccu uauagaggau       60

gacaagaaaa ucguggauau ugguguccgg aucuucuccg acggccgcga ucccaagucu      120

ggggccucac uggcuguuga ccgacgugau gcaagagcca ugaggcggcg cagagacagg      180

uacuugggac ggcgcagugc guuuuuacag gcucuggugg uacacggccu aaugccugcc      240

gaugcagacg aagccaaacu cguggcugau cgagacccau aucaacugag agcaaaggcc      300

cuucaugucg cucuggagcc guucgaaauu gguagggccu uguuucaccu caaucagcgg      360

cguggguuca aaagcaaccg caaggcggau agaaaggcaa augccggaga ggacggcaaa      420

aucgcugaag gcgccaaggc acuggaccag gcuauguccg aggccggggu ggauacacug      480

ggacaguuuc ucgcguuaaa agagacuaag aggguucggc uggguucuga cggccaagcc      540

uacgauuucu auccccagcg acgccauaua gaauacgagu uugacagaau cuggaaagug      600

cagucggaau aucacccugc acuuuugacg gaggcugcca gggcugcccu gcaucggauu      660

cuauucuuuc agcgcccacu caaggcacaa gaggucgggu ucuguaccuu ugccggagug      720

aacggcaucg cugcgaauga aagaaggcug cccaaggcac acccucuuuu ccagcagcgu      780

cggcuguacg aggaaguaaa ccaguugcga auuguuucag ccggugcugc cgauagacca      840

cucacacugg acgagaggga ucgcuuaauc cugcggcucc aagacagaaa gaaggugagc      900

uuugauacuc uugccaaaaa ggaccugagg uuggcagaug gggaacgcuu caauaaagag      960

agugagaacc gaaaggaacu aauaggagac gagguccggg cugaaauguc cgacaaaaag     1020

cguuucggca ccagaugggu gcacuuuggc cuggaugagc agcucaggau ucuggccaaa     1080

cuucaggacg aagagaaucc cgaggcacug uuggauuggc uccgcucuga auaugggcug     1140

gacgaggcgg cugccgaaag cguggcaaac gcccgguuac cugagggaua cgguagauuc     1200

ggcgaaacag cuaccaggcu gcuacucgau gcccugaaag cagaggucau cacuuacgac     1260

aaggcugccc uucgagcguu ugggcaucac uccgauuucc gcacaggaga gguuuuggac     1320

cggcugccau auuacggcga acuccugacc agagagauug ccccggguaa acccgaauau     1380

ggggauccuc uugagaggca auacggcaag aucacgaauc caacugugca uaucggacug     1440

cgucaguugg agaaacucgu aaacgcugug auugcagucc acggccgccc cgaccagaua     1500

gugguugaac uggcccggga guuaaagcug aaugaaaagg agaaagauga acacaagaga     1560

aggaucaaac gagacaccga ugcugccauu gcacgggggc gcaagcuagc gucagaggga     1620

uuuaucgaca acggugccaa uagaaugcuc augaggauau gggaggaacu gaacccugcu     1680

aacccacuug accggcgaug ccccuauugu gccgagccga uuggcaucag agcauuguuc     1740

aguggggaag cugauaucga ccauauuauc ccuuacagcc gcucucugga ugacaaugug     1800

ggaaacaaag ucguggccca cagguccugc aaucgugcaa agggcaacaa gacacccuau     1860

gagaaauggg guccacauau ugauccugaa cggugggaga gaauaucaga ccagguagcc     1920

aggcuccaca agagcaaaca auggcgcuuu gggcccgaug cuauggagcg aaucgacaga     1980

gauggcggau uccuggcgcg gcaguuaacu gacacccagu accugaguag gguugccggc     2040

aaguaccuuc gcucuuugua uccaacagca caguccgggc guguggaugu cauucccgga     2100

agaaugaccg cuaugcuccg gaggaaaugg ggucugaaua gccuacugcc ugaccauaac     2160

uacguggaaa augagcacuc gaacgcccca aagaaucgcc ucgaucaccg acaucacgcc     2220

aucgacgcug cagucgccgc ggugacgacu cugucucuua ugcaacagau cgcugacgcc     2280

gcaggcagag ccgaagauaa gaacuuggac cggcuguuug aggauauucc gcagcccugg     2340

ccuggguuca gggaagaguu aggagaggcu cuccgcucca caaccguuuc acauaaagca     2400

gaccacggca gaaagaggcg gccaagucgu gaucgcgacg ugacugccgc gcgacugcac     2460

aaugauacag cuuauggucu uaccggggac guagccgcag auggcaaaac gcccauaguc     2520

gugcauagag uuccucuggc cagcuugaag ccagcugacc ucaucgaucc ccugaggauu     2580

ccggacccug ugcuacggga cgcccuguac gcagcuaccc gcgauagaac aggaaaagaa     2640

uuugagcagg cccucacuca auucgcgagg caccauccac uguuuaaggg cauccggcga     2700

gucagagugc gcgaaccccu uaacguaauu ccuaucaggg acgccgcagg gcgggcuuau     2760

aaagccuaca agggagaugc uaaugcacgu uucgacgugu ggagauugcc caacggcaag     2820

uggguuuccg auauagucuc uauguucgac gcccacugua uggccgauaa aagcgacagg     2880

cgcccacauc cugcugcgaa gaaagugcug ucauuacggc agaaugauau gguggcaauc     2940

gagcgaagug gugucaccca gauugccaga guugugaagu uuuccacaaa cgggagcauc     3000

gcucucgccg cagacaacga ggccggagcu cugaaagcga gggaugccgc acccaaugac     3060

guggauccau ucaaguauau cuuuucuuca ggcgguggcc uuaagaaccu gaaagcucgc     3120

cagguacggg ucgacccgcu agggagagug uucgacccug gaccccguga auga           3174


<210>  2655
<211>  3177
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2655
augaagaaca gcuacauccu gggccucgac guggggauug ccuccgucgg auauggucuu       60

auacacucug auucaaaaag uguuaucgac gcuggcguga gacuguuccc cgaggcaaau      120

guagaaaaca augaggggag gcgggccaag cgcggaagcc gacguuugaa aagaaggcgg      180

auucaucgcu uagauagagu gaaguaccug cuagcgcagu aucaccucau cauugacgau      240

aaaaucccua aguccaccaa cccauacgcu auaaggguca agggccuguc uucggcccuu      300

ucaaaaagcg aacuggugau cgcauugcuc caucuggcca agcggcgcgg uauucacaau      360

guuaacgugg ucauggacga gaaugaaagu acaaacgagc uguccacuaa agagcaacuc      420

aaggaaaaug cuaaacaguu agagaaccga uaugugugcg aacugcagcu ugauagauug      480

aaugagcagu acaagguaag gggagagcgg aaccguuuua agacggaaga cuucguucgc      540

gaggcaagac aaauccugac cacacagcag aaauaucaug auauugacga acacuuuauc      600

gagcaguaca cucaccuacu cgaaaccagg cgggaguacu augagggacc gggcaagggc      660

ucucaauacg ggugggaagg agaucugaaa cagugguaug agaugcuuau gggucgaugu      720

acauacuucc ccgaagaacu gcgcagcgug aaguaugccu acaccgcuga cuuguuuaau      780

gcccucaacg aucugaauaa cuuagucaua gcgagagacg aaaacgagaa acuggaguau      840

uucgaaaagu accauauuau cgagaaugug uuuaaacaga agaagucccc uacucucaaa      900

cagauugcaa aggaaaucga cgugaaagag ucagauauca agggcuacag gauuacaaag      960

aacgggaagc cacaauucac caguuuuaaa cuuuaucacg accugaauaa aauauucuug     1020

gauaagaaaa accuagaaaa uaucgagcug cucgacgaga uugccuuuau ccugacgcuu     1080

uaccaggauc cccugagcau aaaggaagca uuggaccagc uccaagaucu guuaacugag     1140

ucugaaaaag aggccauugc acaucugaac ggauauaccg gcacacaccg gcuaucccuc     1200

aagugccugc accuuuugau cgacgagaug uggcagacuu cacgcaauca gauggaaguc     1260

uucaccgcuc ugaaccucaa gccucagaaa uaccaacuga gcaagcagaa acagauccca     1320

aagaaaaugg uugaggaauu uauucuuagu cccgugguaa agagaucuuu cauccagucc     1380

auugagauag ugaauacaau cauuaagaaa uauggucugc cugaagagau caucauugag     1440

uuggccaggg aaaagaacag cgaggaucgu cggaaauucc ucaaucgaau acaaaaggaa     1500

aacgagaaua ccagaaaaca ggucgaagag gugaucaggg aguacgggaa ccgcaaugcg     1560

aagggacugg uugagaaaau uaaguuacau cagaugcagg agggcaagug ucuguauucg     1620

cuacaaaaca ucccacucga cgaucugcuu cggaacccgu cucacuacga aauugaccau     1680

aucauaccca gauccguguc auuugacaau aguuucaaca auaaagucuu ggugaagcag     1740

gaggaaaaca gcaaaaaggg caauaggacg ccuuaccagu aucugaacuc caaugagucu     1800

guacucagcu acguucaguu uaaacaacac auccugaacu uaucaaagag uaaggagcgc     1860

auuuccaaaa agaaacgaga auaucugcuu gaggaacggg auaucaauaa guucgaggug     1920

cagaaagagu uuauuaaccg uaauuugguc gacacuagau acgccacaag ggaacucgug     1980

cagcugcuaa agagcuauuu cucugcaaac gaucuggacg ucaaggugaa aaccaucaau     2040

gggucauuua cuaacuaccu ccgcaaggcu uggaaauucg auaagagucg gaauaaagga     2100

uuuaagcauc acgccgagga cgcucugaua auugccaacg uuagcuaucu uuucaagcaa     2160

uccaaagaau ugaaggaggc aaacgccauc cuggaaaaua acaauuuauc ucucaacaaa     2220

aauaaggauu cgaaaaaccu gcuuacagag caggacuaca gaaagauguu ugagauucca     2280

aaacaggugc aggauaucaa ggacuacaag gauuucaaau auagccacag gguagacaag     2340

aaacccaauc gacaacugau aaacgauucc uuguacucaa cccgcgaagu caaugagaac     2400

cauuauaucg ugcagacggu uaaggacauu uacgcuaaag acaauaccaa gaucaaggau     2460

cucuuucuga aaacaccuga aaagcuacug auguaucagc acgacccaca gacuuucgag     2520

aaacucaugc uggugauuaa gcaauacggu gaagagaaaa accccuucgc gaaguauuac     2580

gaggaaaaug gcgaguaccu uaccaaguau gcuaaaaacg aaaaggggcc gacaaucaaa     2640

cgguugaagu acauagggaa aaaggucggc acucaucugg auauuaguac caauuuuucu     2700

ggugccaaga gaaaaguggu acaguuaagc aucaagccuu ucagguuuga cguguuccac     2760

uccaaagcug gguaucacau gcucacacug accuacaugg auaucaagaa aaaggagaag     2820

cauuaucaga uucuuaaaaa cgaauaccag gcccugaagg agcguuauaa aguucccuca     2880

gacgcacaau uuaucggcag cuucuacuac aacgaucuaa uuucuauaaa ugacgagcuc     2940

uuucgcguca ucggagugaa caauuccacu cggaacgugg ucgaacugaa uaugguugau     3000

auuaacuaua aggacuacug cgauuugaug ggcaucaaaa agacgccaag aauuuucaaa     3060

acaaucacca agaagacugg gaaaauagag aaguauagua ccgacauccu gggaaaucag     3120

uuuaaaguga agccuagcaa aucuccccag uuaauuuuca agaggggugu gcucuga        3177


<210>  2656
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2656
augagaaggc ugggccucga caucgggacc aacagcauug gauggugccu uacacagggu       60

cccggcgaug aggccgacgg gauaguggau aucggagucc ggauuuucuc cgacggccgc      120

gauccucaau cugguacuuc acuggcuguu gaccgacgug augcaagagc cgcgaggcgg      180

cgcagagaca gguacaucgg gcggcgcagu gccuuuuugg auacguuagu ggcccacgga      240

cugaugccag cagacgccga ugaagcuaag cuaguagcag cccucgaccc guauacccug      300

cgagcuagag cccuugaggc gccccugaca ccucaugaaa uuggcagggc auuguuccac      360

cucaaucagc ggcguggcuu uaaaagcaac cgcaaggccg agagaaaaac uaaugaugcu      420

gacgacggga agaucgccac cgcagcuuac gcccuggaua gggcgaugcu ggacggaggu      480

uucgauacac ucggccaguu uuuaaacgcc caggaaacca aacgggugcg aauggacggg      540

gaggcacaag gauaugauuu cuacccacag cgcagacaug ucgaguauga auuugacagg      600

auauggacug agcaggcuaa gcaccauccc gcccugcuua cagaaaccgc ucgguacgcc      660

uugcaccgca uccuguucua ucagagaccu cuaaaggcac aagugguugg caaauguacg      720

uuugugcacu ccgaggauag gcucgccaag gcucauccac uguuccagca gcgucggcuu      780

uacgaggaag ucaaucagcu ggaggugacu acccccggug aagcgaaccg aaaauugaca      840

cucgacgaga gagauaggcu gguacgcgaa uuaucucaca agaaagcaau ugccuuuacu      900

ucgcuggcug ccaagaucaa gcucgagccu gggcaaucau ucaauaaagc aagcgagacc      960

cggacacagc uugccggaga cgaaguuaga gcugugcugg gcgauaagaa aagguuuggc     1020

ccagccuggg agcauuucga ccgcgaccga caguggacca uugucgaacg guugcuagag     1080

gaagaggauc cggacgcacu gcacgcguuc cucacgcaag auugcgggcu ggacgaugag     1140

cacgcugccg caacugccaa gacaagucuu cccgaaggau acggucgucu gggcgcuacc     1200

gccacugcaa gaaucuuggc ugagcuccag gccgaaguga uaacauauga cgaggcggug     1260

aggcgcugug gguggcauca cuccgaucau cggaccggag aaauucugcc ucaguuacca     1320

uacuauggcg agcugcuaac gagagacauu cccccuggua cccaggaucc aaacgauccc     1380

ccggagaaaa ggugggggcg aaucacaaau ccuacugucc acaucggccu caaccaaguu     1440

cgccagcugg ugaaugccau cguagcugug cauggacggc ccgaccagau ugucguggaa     1500

cuugcaagag aguugaagcu gaacgaaaaa gacaaggagg agcacaauag gcguauacgc     1560

cgggauaccg ccgcugccau caagagaucu gaaaaacuca cacaggaggg cuuuccagac     1620

acuggggcaa acaggaugcu gcuucgacug ugggaagacu ugaauccugc gaacccccuc     1680

gaucggcgcu gcccauacug uggagccccc auugguauga gagcucuguu cgccggcgag     1740

gcagacguug aucacaucau accuuauagc agguccuuag acgauucagc ugggaacaag     1800

guggucgccc aucggaguug caaucgagca aaaggaaaca gaaccccaua cgaaaagugg     1860

agcgacgauc cggaccgcug ggaugccauu acagcucaag uggcgaggcu gcaccguucu     1920

aaacaguggc gguuuggccc cgacgccaug gcaagaguag agaaggaugg uggguuccua     1980

uccaggcagc ucaccgacac ucaguaucug ucacgccuug cuggccgaua cuugagcagu     2040

cuguacccua cgaaggagga aggaucuguu uaugugaucc caggcagaau gacagccaug     2100

cuccggaggc uguggggguu aaaugauauc cuguccgacc auaacauugu cgagaauaaa     2160

cacagcaacg cccccaagaa ucgccuugac caccgucauc acgcuaucga ugcagccgug     2220

gucgcggugu cgaccagauc uuugaugcaa cggauugcua ggacugccgg acaggcagaa     2280

gaccgcaacc ucgaucgacu guuugagggu cuagacccuc cauggcccgg cuucagagau     2340

cagcugcggg acaggcuccu ggccguuacc gugucccaua aagcugauca cgggcgcaca     2400

ggaaccccgg uaccuggcaa ggacgcaacu gccgguagac uucauaauga uacagcguac     2460

ggguugacgg gcgaaaccuc agcuaaagga cugccaauag ucgugcacag gguucccuua     2520

acaagucuca agccugccga caucacugau cccgaccgga uuccagaccc ugugcugcgu     2580

gaugcaaucg agcgcgccac cgcuggcaag auggggaaag acuuuacaga ugcccuugca     2640

aaguucggac gaagcggcgc ucaggagacu gacagaaaag gugaaaggcu guuucggggg     2700

acccgcagag ucagggugcg ggagcccuug ggaguaaucc caauugccga ugcguccggc     2760

cgaguguaua aggccuacaa aggugacucu aacgcaagau ucgauguuug gcgccucccg     2820

gacggcaagu gggucagcga uauaguguca auguuugacg cucacaggcc ugaucaaggg     2880

gaccggcguc cccauccagc cgcuaagaaa gugcugaguc uaagacagaa ugauaugauc     2940

gcaauugaaa gggacggagc uccccgcgag aucguccggg uugugaaguu cuccacgauu     3000

ggcagcgugc agcuggccgc uaccacagaa gcggggccuc ucaaagcacg agacgccucu     3060

ccacccgaug uagacccuuu uagauauauc aacucaagug cuggaggccu gaagaaaaua     3120

aaggccaggc aaguccgcau cgauccgcuu ggucggauuu ucgacccagg gcccagauga     3180


<210>  2657
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2657
augaucgaga ggauucuggg cguggaccuc gggauaagcu cccuuggaug ggccaucguc       60

gaauacgaua aggacgauga ggcugcaaac aggauuaucg acugcggugu ucggcuguuc      120

accgccgcgg aaacacccaa aaagaaagag ucuccuaaua aggcucgccg agaagcccgu      180

ggcauuagaa ggguguugaa ccggcgcaga guaaggauga auaugaucaa aaaguuauuu      240

cugcgggcag ggcuaauaca ggauguggac cucgauggag agggcgguau guucuauuca      300

aaggccaacc gcgcugacgu cugggagcug cgacacgaug ggcuuuacag acuguugaaa      360

ggagacgaac ucgcaagggu gcugauccau auugccaagc accggggcua uaaauuuauc      420

ggcgaugacg aggcugauga agagaguggg aagguuaaaa aggccggagu gguccugcgu      480

caaaauuucg aggcggcagg uugucgcacu gugggcgaau ggcucuggag agagagaggg      540

gccaacggaa agaaacggaa uaagcauggc gacuacgaaa uuagcaucca ccgagacuua      600

cugguagagg aaguugaggc uauauuugug gcccagcagg agaugcgcuc cacgauugca      660

accgaugcuc uuaaagccgc guauagagaa aucgccuucu uugucaggcc aaugcagcgg      720

auugagaaga ugguggguca uugcacauac uucccggagg aacgcagagc acccaaaucu      780

gcuccuacug ccgaaaaguu uaucgcuauc ucgaaauucu uuucaaccgu gauuauagac      840

aacgaggggu gggagcaaaa gaucauugaa aggaagacau uggaggaacu gcuagauuuc      900

gccgucagcc gugagaaagu ugaauuucgg caccuccgaa aguuccugga ccuuagugau      960

aaugagaucu ucaaaggacu gcacuauaag ggcaaaccaa agaccgcaaa gaaaagagag     1020

gccacuuugu uugaccccaa cgaaccuaca gagcucgaau uugauaaggu ggaggcugag     1080

aaaaaggcgu ggauaucccu gaggggcgca gccaaauuac gcgaagcucu ggggaaugag     1140

uuuuacggac gguucguagc ccucgguaag caugcagacg aagccaccaa gauucuuacg     1200

uacuauaaag augagggcca gaagagaagg gaacugacua aauugccacu agaggcugag     1260

augguggaac gccuggucaa gaucggguuu ucugacuucc ucaaacugag ccuuaaggcc     1320

auccgagaua uucugcccgc aauggagucc ggagcgcggu acgacgaagc uguguugaug     1380

cucggcguuc cucacaagga gaaaucagcc auccugccac cguuaaacaa gaccgacauu     1440

gauauacuga aucccacagu gauccgugca uuugcccagu ucagaaaagu cgcuaacgcc     1500

cuagugagga aguauggugc auuugaccgc guacauuucg aacucgcucg ggagauuaau     1560

acuaaagggg agaucgaaga uaucaaggag agucagagaa agaacgaaaa ggagaggaag     1620

gaagccgcgg acuggauugc cgagaccagc uuucaaguuc cucugacacg caaaaacaua     1680

cuuaagaaac gauuguacau ccagcaggau ggccggugug cuuauaccgg agacgugauu     1740

gagcuggaaa gacucuucga ugagggcuac ugcgaaaucg accacauucu gccaaggucu     1800

cguuccgcag augacucauu ugccaauaag guccuuuguc uggcucgcgc caaccagcag     1860

aaaacggauc ggacucccua ugagugguuc gggcacgacg cagcgagaug gaaugccuuc     1920

gagacaagga ccagcgcucc uaguaaccga gugcggacug gaaaggguaa gaucgaucgc     1980

uugcucaaaa agaauuuuga cgaaaacucu gagauggccu ucaaagacag aaaucugaac     2040

gauacaaggu acauggcacg ggcuauaaag accuacugcg aacaauauug ggucuuuaaa     2100

aauucccaua cgaaggcccc agugcagguu cgaagcggca aguuaaccuc ggugcugaga     2160

uaccaauggg ggcuagaguc uaaagaccgc gaaucccaca cacaucacgc aguagaugcc     2220

aucauuaucg cuuucucaac ucagggaaug guccagaagc ucagugagua uuacagguuu     2280

aaagagaccc aucgugaaaa ggagcggccc aaacuggcgg ugccgcuugc caacuucaga     2340

gacgcaguug aagaggcuac aaggauugaa aauacugaga ccgugaagga gggcgucgaa     2400

gugaagcgcu ugcugaucag ccgaccuccc agagcccggg uaacagguca ggcccacgag     2460

caaaccgcua aaccauaucc uaggauaaaa caggugaaaa acaagaaaaa guggcgccuc     2520

gcacccauug augaagagaa auuugaaucc uucaaggccg accguguugc gucugcuaau     2580

cagaagaacu uuuacgagac uagcacgauc ccaagagucg auguguauca caaaaagggg     2640

aaauuccauc uggugcccau uuacuuacac gagauggucc ugaaugaacu uccuaacuug     2700

ucacucggca caaacccaga ggccauggac gaaaauuucu ucaaguuuag uaucuucaaa     2760

gaugaccuga uauccaucca gacccaagga acuccgaaga agcccgcaaa aauuaucaug     2820

ggcuacuuca agaacaugca uggggccaau augguucuaa gcucuauuaa caauucaccu     2880

ugugagggau uuaccugcac accagugagu auggauaaaa agcacaaaga caaguguaag     2940

cugugccccg aggaaaaccg gaucgcuggu aggugucucc agggcuuccu ggauuauugg     3000

agccaggagg ggcuucgccc uccacgaaaa gaauuugagu gcgaccaggg agugaaguuc     3060

gcauuggacg ucaaaaagua ccaaauagau ccccugggcu auuacuauga aguaaaacag     3120

gagaagagau uagguaccau uccgcagaug cgguccgcca agaaacucgu gaagaaauga     3180


<210>  2658
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2658
augagcaaga gacugaggua ccggcucgcc cuugaccugg gcaccaacuc caucggguuc       60

ugcuuguuag aucugaaugg agacggucgc cccuguggcg ugcgacguau gggggucaga      120

auuuuuccug auuucucuaa acuacagcaa ucacucgcug cagacaggcg gcuggcccgc      180

aguaugagaa ggcggcgcga ucgauaucuu agaaggcgga aggcgcugau gaacgcuuug      240

guugccgcag cccucaugcc agcugacccg aaagcacgua aggcccugga ggcucuggau      300

cccuacgaac uccgcgccgc ggcaguggcc ggaccuuuac cagcucacca gcugggcaga      360

gcccuuuuuc auuugaauca gaggcggggu uucaaaagca accgaaaggc agcuggggac      420

gagaaugaag ccggaaaggu aaaauccggc auaucugcgc ugaaggccga gcuagcagcu      480

ucgggcgccg ggacacucgg acaguggcug gcugcccgcc acgcaaacag acaacccgug      540

agggcccggc uuacugguac gggcgcuaaa gcgaccuauu cauuuuaccc ugaacgcgca      600

cugguccaug aggaguucca ggccaucaga gcugcccagg caccacacca ucccggggug      660

acagccgaac agugggauag gauugagagc guuaucuuuc accaacgucc uuugaagcca      720

gugaaaccgg gaaagugcac ucucuauccc gaagaggagc gggcuccuug ggcccugcca      780

auugcacagc gauucagaau cuuacacgaa cugcauaauc ucaggauagu ccgccccggc      840

cagggugagg aagggcuuag uccugcggac uuugaucggu ucuuugacag acuguguagg      900

aagggagauu ugaccuucaa aacacuagcu aaggagcuga aacucccacc cgacgcccgc      960

aucaaccugg aagacgauaa gcgagaccgg cuuaaaggcg augcaaccgc cgacgcucug     1020

uccgccgaga agggcaaacg uagaaggauu ggggcagcuu ggcacucuuu uccggccgau     1080

cgccagcggg agaucguggg aagauugauu gacgcggaag augaggguga acucgccgcu     1140

uggcugguag aggaauuagg ccugccuugg gagguugcaa uccuaacugc cgagaggccc     1200

gggcucccag acggauacug ccgacugagc gaaaaggcuc uugagcaagu ggucccuuug     1260

augcaugagg aacaccuccc cuaugaugaa gccgugcgcg cacucggccu gcaccauucc     1320

gaccaccgga caggugaguu ccuugaugag cugccauacu acggguugcc ccucgaaaga     1380

uucauagcgu uuggcaccgg agaggccgac gacccuccag aacugaggau uggccguuuc     1440

ccgaauccca cggugcaugu cgcuuuaaac gagcugcgcc ggguugugaa uagaaucauu     1500

aggcgauggg ggaagccuga ggaaaucgua guggagcuag cccgggaucu caaacaguca     1560

cgcgcagaca gagauaagac ucagagggaa caagcugaca accagaaaaa gaauuggacc     1620

cggacacagc agcugcgaga gcuuggaaug gaaccaacug gugaggccuu gcugagacuc     1680

cgccuguggg aggaauuacc caggaucggc agugcacacg ucugugugua uaccgggcaa     1740

ccuauuagca ugacacagcu gcuucaggga gauguugacg uggaucauau auugccauuu     1800

ucucguaccc ucgccaacgg cgcugacaau aaaaucgucu gccaccggag agcgaacagg     1860

auuaagcgca accgaacgcc cugggaggcc uucggugugg uauccgcaga uggguacgac     1920

ugggcugcca ucgccgaaag agcugaguca cugccgaaga auaaacggug gagguuucua     1980

ccuaacgcaa uggaacgcuu cgagacugag cguggcuuua uagauagaca gcugguugaa     2040

acacggcacc ucagcagggu ggcccgcgag uaucugggau guauuguccc acuugaaaag     2100

gugcgagcgg cucccggcag auugaccgcc augcugcggg ggaggugggg auuaaauagu     2160

auccucucug acgcaaaccu gaaaaaucgc uccgaucaua gacaccaugc cgucgacgcu     2220

gcagugcuug guguuaucga ccaaaggcug gugcagcggg uagccgaugc ggcuaagcgu     2280

gccgaggaaa aagaguugga gaagcuguuc gacggccugc cugaucccuu ugaagggcua     2340

ccaggaccua gcuuccgcga ccagguccag gagcgagcac aaggcauuau cgugucgcac     2400

aagcccgaac acgguuggca ggggagaaug cauaacgaua cugccuacgg cccaguugag     2460

aggcugccgg acggaacaca ccgggugguc acccgcaaac cucucggcuc ucuggcugag     2520

aaggauuuug ggaauauuag agacagggcc cuugcagcug ccuuggaacg gcauguggcg     2580

gagcaggccg cacagggacg aucccugggc gaagcucuuu caugcuucac guggaccggu     2640

ggggagguaa gacgcgugag gaugcucgau aaacugggaa gucccguugc cguccaagac     2700

gaugacggcc acacauuuaa guuauauaaa ggugauagca acgcuuggua cgaaauacug     2760

cgggacggcc gugggagaug gacuggaagg aucgugucca ccuaugaggc auaccgccua     2820

caugccgagu ggcucgccga acggggcgac gggggacgau cugaugcugc guucccagac     2880

ugggcagcca gaaggcugug ugcuggcauu gguaagcccu uguuccgccu guuuaagggg     2940

gauauguuag ccuucggaac agcaccuaau cgggagauca ugaaaguggu caaguuuagc     3000

gaaggcagaa ucguuuucgc cggucaccgu gaggcuggca accucaaaga aagggacgcg     3060

gauaaggacg auccauuuaa auaccggacu ugcucacccg aggcccugcg caaggcacag     3120

gcucgaccug ugggggugga cccggccgga uucguacacg auagaggccc acuugaguga     3180


<210>  2659
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2659
augggcauca gauucgccuu ugaccugggg accaacagca uuggaugggc uguguggagg       60

acaggucccg gcgucuucgg ggaggauacu gcagccuccc ucgacggauc uggcguucuu      120

auauuuaagg auggucggaa uccuaaagac gggcagucac uggcgacgau gcgccgagug      180

ccacgucaaa guagaaagag gcgggaucgc uucguauuga gaaggcggga cuuacuggcu      240

gcccuacgca aagcaggacu cuuuccggug gaugucgaag agggccgaag acuggccgcu      300

accgaccccu accaccuuag ggcaaaggcc cuggaugaaa guuugacacc ucaugagaug      360

ggccggguga ucuuccaccu caaccagcgu cgcggguuua gauccaauag gaaagcugac      420

cggcaggauc gagaaaaggg aaagauugcc gaggguucua aacgccuggc ggagacucug      480

gcagccacca acugcagaac acucggcgag uucuuauggu cgaggcaucg ggggacccca      540

cgcacuagau cacccacaag gauccguaug gagggagaag gcgcuaaggc ccuguaugca      600

uuuuacccua cccgggagau gguucgagcu gaguucgaaa gacuuuggac ggcccagagc      660

agguuugcgc cagacuugcu gacucccgag cgccacgaag agauugccgg uauccuauuc      720

cggcaaagag aucucgcacc uccaaaaaua ggguguugca ccuuugaacc gagugagagg      780

cgccugcccc gagcccuucc uuccguggag gcucggggaa ucuaugaacg ucuggcucau      840

uugagaauua caacuggccc agucucugac aggggccuca cccgccccga gcgggacgug      900

cuggccagcg cauuacuggc cgggaagucc cucacauuca aagcuguaag aaagacccuu      960

aagauccugc cucacgcguu gguuaauuuc gaagaggcag gagaaaaagg ucuagauggc     1020

gcccugacgg cuaagcuccu gucaaaacca gaccacuacg gggccgcaug gcauggacuu     1080

aguuuugccg agaaggauac uuucgugggc aaacuguugg acgaggcuga ugaagagagg     1140

cucauucgac gccuggucac agaaaaccgg uuaagcgagg acgccgcaag aaggugugcg     1200

ucuauccccc uggcugaugg uuaugggcgu cuaggccgca ccgccaauac ugaaauacuc     1260

gcagcccugg uggaggagac agacgaaacc ggaacggugg ucaccuacgc ugaggccguu     1320

cggagagcag gcgaaaggac agggcgaaac uggcaccauu ccgaugagcg ggacggagug     1380

auucuugauc gcuugccgua uuacggugag auccugcaga gacacguagu gccuggcuca     1440

ggggaacccg aggaaaagaa ugaggcugcc agguggggac ggcucgcgaa cccaacuguc     1500

cacauuggcc ugaaucagcu ucgaaaagug guuaacagac ugaucgccgc ucauggucgc     1560

ccugaccaga ucguggucga auuggcaagg gagcucaagc ugaaucguga gcaaaaggaa     1620

cgguuagaca gagagaacag gaaaaaucgc gaagagaacg aacgaagaac cgccauucug     1680

gcugagcacg ggcagcggga uacagccgag aacaagauaa ggcuacgccu cuuugaagaa     1740

caggcacgug cgaaugccgg caucgcucug ugccccuaca cugguagagc cauuggcauc     1800

gcagaacuuu ucaccagcga gguggaaaua gaccauauuu ugccaguaag ucugacacuc     1860

gaugacucuc uggcuaaccg gguuuuaugu aggcgcgagg ccaaucgaga gaaaagacgg     1920

cagacccccu uucaagcauu cggggccacu ccugcuugga acgauaucgu ggcgagggcc     1980

gcaaagcugc caccgaauaa acgcuggaga uuugaccccg cugcccuuga aagguucgag     2040

cgggaaggag gauuuuuggg ccgucagcuc aacgagacga aguaucuguc ccgccuagcc     2100

aagaucuacc uggggaaaau uugcgauccu gaccgagucu augugacacc aggaacccuc     2160

acuggccugc uuagagcuag gugggguuug aauagcaucc ugucggauuc uaacuucaag     2220

aaucgguccg accaccgcca ucacgcaguc gaugccgugg uuauuggggu guuaaccaga     2280

ggcaugauac agaggaucgc gcacgacgcu gcccgggcag aggaucaaga ccucgaccga     2340

guauuuagag auguccccgu gccuuucgaa gacuuucgcg aucauguuag ggagcgggug     2400

ucaacaauua ccgucgccgu gaaaccagaa cacggaaagg gcggggcucu gcaugaggac     2460

acuagcuacg gacuuguacc cgauacagac ccgaacgcag cccugggcaa uuuggugguu     2520

cguaaaccua ucagaagccu cacggcgggu gaagucgaua gggugcgcga ccgggcucug     2580

cgagccagac uaggggcacu ggccgcucca uucagggaug aguccggacg cgugcgggac     2640

gccaagggcc ucgcacaggc ucuggaggcc uucggugcgg aaaacggcau cagacguguc     2700

aggauucuua agcccgaugc cucuguugug accauagcag accggcgcac uggggugccu     2760

uaucgagcug uagcccccgg agagaaucac cacgucgaua ucgugcagau gagagacggc     2820

agcuggaggg gguuugcugc aucaguuuuc gaagugaacc ggccaggaug gcgcccugag     2880

ugggaaguca aaaaguuggg cgguaaacug gugaugagau uacauaaggg ggacaugguu     2940

gagcucagug auaaagacgg acagaggcga gugaagguag uccaacagau ugagaucucc     3000

gccaaucggg ugcgucugag cccccacaac gauggcggua aacuucagga cagacaugcc     3060

gaugcugacg auccauuucg cugggaccug gcgacaauuc cgcuacucaa ggauaggggc     3120

uguguggcag uccggguuga cccuaucggg gugguaaccc ugagacgcuc uaacguguga     3180


<210>  2660
<211>  3183
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2660
auggccguga aaagccugaa cuacauccuc ggccuugacc uggggauugc uuccugcgga       60

ugggcagucg uugagaugga ugaacaggag aaucccuuga gauuaauaga cgugggugua      120

aggaccuucg aagaggccga aaacccuaaa gauggaucuu cacuggcgga gacacggcgc      180

cuagcucgag cccaacguag acucaucagu aggcgggcaa aucgcaugaa caagcugaga      240

aggcuucuga aacaggagaa uguguugcag gccaacgacu augaugaaaa ggggcucauu      300

aucggacugc caaaucaggc uugggagcug cgggcacaag gccucgaccg caaauuagaa      360

ccgaaggagu gggccgcugu ccugauucac cuugugaagc aucgagguua cuugagccag      420

agaaagaacg agucccagac ugccgauaag gaacuggggg cgcuacucuc uggaaugaau      480

cacaaccauc agcugcuuca aucgcaguca aaucaguaua ggacgcccgc agagcuggcc      540

aucaaacagu uugcuaccca agacggccac auacggaacc agcguggcgc cuacacacau      600

acuuucaauc gcuuggaucu ccagaacgaa cugaaucaau uauuugacgc acagaccaga      660

cuggggaacc cucacguuag cggaagucuc aagacagcua ucaacgaucu ucugaugaau      720

cagaaagccg cguuguccgg ugacgccauu cuagagaugc uggggaggug uaccuucgaa      780

ccaucugagu auaaggcagc uaagaacacu uacagcgccg agcgguuugu guggcucggc      840

aaacugacaa aucuucgaau ccaggauaac ggacuggaau ccgcuuuguc acgcgagcaa      900

agagaacucc uguuaaauga gcccuauaac aagaccaaac ugacguacca gcaggucagg      960

aagcuacucc ggcugccuga aacugccuuc uuuaaaggcc uucgcuacgg uaaggacaau     1020

gcagagagug ccaccuugau ggagaugaaa agcuaucacg cuauuagaaa gucucuggaa     1080

aacaaguccc ucaaaucaga gugggaaaag cugaaaacac agccaaaacu ucuggacuug     1140

aucgggacug cguucagcau auacaaaacc gaugacgaga uugcaggaug ccucaagaau     1200

caacugcccg aaaacgugau cauugccuua caggagcagc ugaauuuuag uaacuucaug     1260

gagcuaucuc ucaaggcucu ggaaaaacuu uugccucuga uggagcaggg ccucagguau     1320

gaugaagccu guacacaaau cuacggcaau cgucaugaga agaucgcaga caaaaccgag     1380

aaguuucugc caaacauucc ggccgauaaa uuacggaauc ccguaguucu gcgaacgcuu     1440

acucaggcua gaaaggugau aaacgccauc auuaggcgcu augggucccc ugcacggguc     1500

cacaucgaaa caggaagaga gguggguaag agcuucucgg acagggauaa auugaagaaa     1560

cagcaggaag agaaccaaga acagcgccga cgggcgauag acgaguggaa gcaguuuuuc     1620

ccaaaugcug cccaugagcc caaagcagug gauauucuca agcugcgucu auaccaacug     1680

cagcagggca agugccucua uucugggcug ccuaucgacg ucagaaggcu uccagaaaaa     1740

ggauacguug agaucgauca cgccuugccc uucucccgca ccugggacga cucauuuaac     1800

aauaagauuc ugguguuagc uagugaaaac cagaauaaag gcaaccaaac uccguacgag     1860

uaucuccggg gugccgauaa uagcgaaucc uggagacugu ucguagagaa cgugcagaau     1920

ugucaguuuu cuccuaacaa gaaacagaag aucauggcaa aacaacuuga cgagaagggg     1980

uuccuggaaa ggaauuugaa cgauacacga uacgucagcc gcuuucucug ccaguucauu     2040

ucagaguauc ugcaccuaac cggcaagggc aaaaagcggg uguuugcuag uaauggccag     2100

auaacggccc ugcucagagg gaggugggga cuguccaaaa accgugaaga gaaugaccgc     2160

caucacgcgg uugaugccgu ggucguggcu uguagcaccg uagcaaugca gcaaaagauc     2220

acacaguucg uucgguacca gaaagguaac guguuuacug gcgaaagaau ugacaaggag     2280

accggggagg ucaucucuau ccauuucccc acaccauggg aacacuuuag ggaggaagug     2340

augauucgag ucuucgauga caacccucuu cggacuuugc ccgagaagcu gccaaaucgc     2400

cccgaggccu uacaugaaua ugugaugccu cucuuuguuu caagaaugcc aaccaggaaa     2460

augacaggac aaggccacaa ggagaccaua aaaagugcua agcggcugga ugaaaagauc     2520

agcgugcuuc gaguaccgcu gacucagcua aagcucaacg accuggagaa uauggucaac     2580

agagaacgcg agcccgccuu guacgaggca cugaaggcga gguuagaaca guucaaaaau     2640

gauccugcca aggcuuucgc cgagccauuu uucaaacagg gugggcaaca ggugaaggca     2700

guucgugugg aaauugucca gaaauccggc augcuccugc ggaagaucaa cggaguggcu     2760

gacaauggcu cuaugguaag aguggauguu uuugagaagu cggggaaauu cuaucuuguc     2820

cccauuuacg ccuggcaagu ggcagaagga auccugccua accacgccgu gguccauagc     2880

aaggacgagu ccgacuggau auugauggau gagucauacc acuuuaaauu cgcucucuau     2940

aagaaugacc ugcuagaagu ucagacgaaa aaggguugca ucaugggcua cuuugcgagu     3000

cuggauaggg ccacaggggc aauuaccauc cgcgagcaug accgagauaa aaagaagugu     3060

aaagacggag aacacagauc uauuggcgug aagauggcua gcuccuucaa aaagcuccag     3120

guggaugagc uugguaaaaa cauccacgaa acucggucaa ggaagcgccc aaauauaggg     3180

uga                                                                   3183


<210>  2661
<211>  3186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2661
augcacaccc ugcccuacac acucggccuu gacaucggga uugccagcgu gggagcugca       60

cuguuguccg augagucuau auuagguaug caugucagaa cuuucucagc cgcggaaacg      120

gacaaggagg gcgauccucu gaacaaaauc aggcgggaag cucgcagugc ccgacguaga      180

auuaggcggc gcgcacacag acuacucagg cugcggcgcc uucugaagcg aaccggguug      240

aucagcgaca caucccucga gguucugaaa acuucuaaga ccccauggca gcugagaucg      300

gaaggacucg auagguuacu gucaccgagc gagcuugccg cugcauugua ucauauugug      360

aaacaccggg gcuuucaaag uaaucguaag gccgagcuga agacagacgc uaaagccggu      420

cagaugcuau ccgggguauc ugcgaaccag cagcucaugc gcgauaagag cuacagaacc      480

aucgcagaaa uggugugcaa agacgaugcc uucaaggacc auaaaaggaa uaagggaggc      540

uccuaucgga acacuuuugc ucgaucagau cuggccgacg agcuuaagac acuguugaaa      600

aagcaacgcg aauacgauaa uccccucgca accccugagc uggaggaagc uguccacacg      660

uuacugaugg ccagaaggcc aguguucaaa gacgacgaua uagagaagcu cguuggcuuu      720

uguacuuucg aacacgggga gaaacgggcg cccaaggccg cauuuaguag cgaacgcuuc      780

aucuggcuug gaaaacugaa caauuugaga cuaauuuauc cuggugagca gaggucucug      840

accccagagg aacguuccca gaucauuaac cuccccuuua cacaggcuuc agugaaguuc      900

agccaaaucc ggaagaaacu ggaccuuccu gaguacgucc gauuuaaucu ggugagauau      960

aagccaggcg aagagacuag caaaucugaa accgccacau ucuuugaggc uaaggccuuc     1020

caugaguuga ggaaaaccua ccgcgaaucc gggauaagcg agcucuggga acgggaucag     1080

cacgacccgc agagacugga ucagauugca acggccaucg cuguauacag ggacgaugag     1140

cgcauucgaa acgaauuauc gccccauacu aucgagaaug agguugugga agcgguccug     1200

aacugcgacu ucacacaauu uaucaaucua ucucucaagg cacugaacaa gauucuuccu     1260

uauauggagc agggacagcg guacgaugaa gccuguaccu ccgcuggcua ucaacaccac     1320

ucaccacagc agaaugagga gaaaaguggu cguauacccc cuccagacaa gaacgugauc     1380

agaaaccccg uggucuacag gagcuugaau caggcccgca aacugauuaa cgcaaucacu     1440

aagcgguaug gggccccggc ugccauacau cucgaaaugg caagagaucu guccaaaccu     1500

uucucugaga ggaagaguau ugaacgagag caagaaaagu accgcaaaca gaagguucag     1560

cuucggaaug aguauaucga gcacuuugga acagaaccca acggccugaa uuugcucaaa     1620

uucagacugu acaaggagca ggacgcgcaa ugcgcuuaca cccagaaagc cuuagaccug     1680

ucaaggcuaa acgaagcagg cuauguggag guagaucaua uccucccaua cagucguucc     1740

uaugacgaua gccugcuuaa uaagacguug gugcugaccg aagccaaccg cgacaagggg     1800

aaucggacac cuuuugaguu caugggaggu gaugaccagu cucagcacug gcaaaaauuu     1860

gucagauggg ugcagaugaa ccccaagauu aggcaggcua aacgaaaucg guaccuccgc     1920

aaggauuucu cagagaaagc cgcaagagaa uuuagggacc ggcaucugaa cgauacucga     1980

uauaucggca gagaguuaaa ggcuaugauu gaaagucacg uucaguggca cccagacagc     2040

aaauccaagg auuguugcgu gguccugucu gggcaacuua ccgccuugcu ccgcggaagg     2100

uggggcuuca auaaggaccg ugcggccggu gaucugcauc acgcugugga cgcagccgua     2160

guugcugccg caacacgggg gaugauacag agaauggcga ggcauucgaa auuucacgag     2220

cuaaagaacg ugcgcacuag cuacgccgac cccgauaccg gcgaagucgu gaaucuggcu     2280

gccgcacgag accucgagaa acacuucccu cugccauggg auaacuuuag aaacgacaca     2340

cuugcuuauu ugaauccguc cgccaucuca gcagaugaga gugcccagga aggacaucgg     2400

ggcgucaggg ugucucgcgc ucccacccgu agaaagcugg gggcggccca ccaagagacu     2460

auucggagca aaggaaagga caacaucuua cucgaacagg gucuguccgc aguuaagacg     2520

ccucuuucaa aacugacauu gaagaauauc gagaacaugg ugggcuacga ggauccaagg     2580

aaugcugccc ucguaaaagu ccugcgcgaa cgacuagagg ccuucggggg agacggcgcu     2640

aaggcauucg cccccaacca gccuccagug uacaaaccca gcaagccggg uaagcaggcg     2700

ccugaaauua gaucuguuaa aauggcuucc aagaguaaag gggcccugca ugugcgggag     2760

ggccucgcag auagcggaag gaugauacgc aucgacauuu augaaaccaa gaauggcuac     2820

gucggggugc caaucuaugu aucugauaaa gccagaucca agcugcccaa ggcuuacccu     2880

aaaaagggag aggacagggc aaacuggccc gagguggaua cuaaugacaa cuuucuuuuc     2940

ucauuguaua guaccgacug gauugaagcc aaaacaaaga aagagaccau cgcggguuac     3000

guuaagagcu augauauauu ugacggccgg cuguuagucu acgcucacga uaauaaaaag     3060

guggugcgua agcucacucu gucggcccuu gcagaccugc aaaaacuaca ugucuccauc     3120

cucggggauc uguacaaggg aggcacacca aaauguucuc cuuugcgcaa gggucaggaa     3180

cuguga                                                                3186


<210>  2662
<211>  3186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2662
augcugagac ucggccuuga caucgggagc accuccauug gaugguuccu guacaggaua       60

acaaacggua ugaucgccga gauuaucgau ugcggcgugc gguuguuuuc ugacgggcgc      120

gaugcuaaga cuggagucuc aagugcaguu gaacgacgua augccagagc guuaaggcgg      180

cgcagagaca gguaucugcg gcgccgagcu gcccuaauga gaaggcucgc agccgcuggc      240

cugaugcccg aggauccuag ccaggcaaaa ccacuugaac uguuggaccc guaccggcuc      300

cgugccgaug cucuggaccg cagacuggag cucacggaau uagguagggc ccuguuccac      360

cuuaaccaac ggcgaggguu uaaguccaau cgccagaccg auagaggaaa caaugagggc      420

ggcaaaauua ucgacggguc ugcgagguug gaucaggcaa ugauggccgu gggagcucgg      480

acauauggug aguuccugca ucuacgcaga ucgcaggccg aagaccucag gcguguaccc      540

ucagugcgga cucgacugac ccuuagaagc gucgauggcg ggaaggcaga gacaggauac      600

gacuuuuauc cugacaggcg ccaccuggaa gaggaguuca acaaagugug ggcugcccaa      660

gcggcccauc acccaguuuu gaccgauagu cuccggcaug aaguguuuga gacuauauuc      720

uuucagagac cccugaaggc uccugcaguc gguagguguu uauucuuucc agaaccccgc      780

cugccucgag cccacccacu cacacagcgg cgugugcuuu acgagaccgu aaaugcucug      840

agaaucagga cggaauccgg cgccgcaguu ccguugggga aggagcagcg cgacaaagug      900

auuaugcuac uggauggaaa ggcccccguc aaagcuaucg cgucugcaaa gcucagccug      960

aaagccauug cuaagcaccu uaagcugcgg gacggccaaa cuuucaccuu ggagaaaggc     1020

agagauuaua ucgccugcga cccugugagg gcaucccucu cacauccaga ucgauuuggg     1080

ccccgcugga gugugcugga agccgagguc caggcugaaa uaguuggacg gauuagagac     1140

gaugacgaua caauggccuu aguggagugg cugaagcagg uacacggucu agacgacgca     1200

caugcgaggg cuacugccga ugcaccucuc ccagaaggcu acgggcgucu gggagagacc     1260

gccacacgca aacuuuugga cugucugaag gcugaggugc ucaccuacag cgaagccguc     1320

ggcaaagcac uggaucagca ccacucugac cggagaacgg gugagauccu ugaaucccug     1380

cccuauuacg gggagguguu gcaagcucau guuauuccgg gcacuggaga accucaccau     1440

gaugacauca caagguucgg ccgaaucacc aaccccacug ugcacauugg gcucaaucag     1500

cugcggcgcu uagucaacag aauaaucgcc agguauggaa agccagcgga gauuguggua     1560

gagcuggccc gggaucuaaa acagucaaca ggucaaaaga aggcugcaau cgcccucaau     1620

cugaaaaacc gaaaggcugc cgaagcaaga agcgcgcuuu ugaccgagug gggcguuccu     1680

gacacggggg ccaaucgcau guacgugagg cuguggcagg aacuccauga ggaucccaug     1740

augcgucggu gcccauauac cggaacaaua auuagucccu cucuguuauu cuccggcgcu     1800

aucgacaucg aucacauucu gccuuacagc agaacucuug acgauucgac cgccaacagg     1860

acauugugua ugaaagaggc aaaucgccag aagcgaaaca aaucuccauc cgaagcuaga     1920

cgggacggua ugaggaucug ggcccucgag gacgauaacc ugcgccacau uccggaauuu     1980

aagcguugga gauucgcaga ggacgccaug gcucgguuug aaggggagag ggauuucgag     2040

gcgcgcgccc uagucgacac ucaguaucug ucacgaauaa guaaggcaua ccucgaaacc     2100

cuguacgaug gcgcugacgg aaaaagccau gugugggucg ugcccggcag acuuacagag     2160

auguugcgga ggcacugggg gcugaaugga aucuuaaccg auccucuccg cgacguuacu     2220

aaggagaaaa acagaaugga ucauaggcac cacgccauug acgccgcugu gguagcagcc     2280

acggaucggg gucugaucaa ggcgauggcu gaacaagccc guacagacgu cgaucagggc     2340

cgcgaagcac uugccuccuc uaucaugcca cccuggccug gguuucgaga ggcuguggca     2400

gcccagguug acagaauuau agugagccau aggccagacc acggacggau cgaucugacc     2460

acucgcauga aaggcagaga ccagaccaca ggucccuugc auagggauac cgcguauggg     2520

cucacuggcc aaucagagaa gggagucccg cuggugguaa cacgggaacc uuucgacaau     2580

cuaacgccaa ccaugauugg caagauccga gaugagcccc ugaaagcugc ccucgcagcc     2640

gugacugcug ggaagaaaga cgaugccuuu gcagaagcuc uggcccacuu cagagaccgc     2700

ccuggacccu accauggcau uaggcggguu cgucuuaucg agccaauaag ugucguggaa     2760

aucagagaug cguccgguag ggccuauaag ggguacaaag gaaacagcaa ucacugcuuu     2820

gagguguggc gcuugccugg cggugcaauu acagagcaca ucgucucuac cuucgacgcu     2880

caucagcccg gcccagaacc gcggccucac cccgccgcua agcgacuguu aagacucuuu     2940

aagucagaua ugguuaaacu ggacgacagu aaguucgggc cagugauugc aacaguggua     3000

auguuugaua ggaacggaaa aaucagccuu gucccccaua cugccuccaa ugcccgcaag     3060

cggugugaga aauauaagga agaccuguac cuaagauuca ccgcucguac gcucauagag     3120

gcgggcgcaa ggcggauuau cguggaugag accgggcacu uccgcgaccc uggacgaaaa     3180

ggcuga                                                                3186


<210>  2663
<211>  3189
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2663
auggccgaga agcagcacag auggggccug gacaucggga ccaacagcau uggaugggcu       60

gugauagcac ucaucgaagg uaggcccgcc ggccuugucg cgacaggguc ccggauuuuc      120

ucugauggac gcaauccuaa agacggcuca agucuggcug uugagcgacg ugguccaaga      180

caaaugaggc ggcgcagaga uagguacuug cggcgccgag acagauuuau gcaggccuua      240

aucaacgugg ggcugaugcc gggagaugca gccgcuagga aggcccuagu aacugaaaau      300

cccuaugugc uccggcagcg uggccuggac caggcacuua cgcugccuga guucggccgc      360

gcuuuguuuc aucucaacca aagaaggggg uuccagagca aucggaaaac cgaucgagcc      420

acagcgaagg aauccggaaa agucaagaac gcaauugccg cuuuucgcgc cgguaugggc      480

aaugcaagaa cuguggggga ggcucuggcc aggcggcugg aggacggacg cccaguuaga      540

gcgaggaugg ugggccaggg uaaggaugaa cacuacgagc ucuauaucgc ccgugaaugg      600

auagcacagg aguucgacgc uuuaugggcc ucucaacagc gguuucaugc ugagguccug      660

gccgaugcag cccgagacag acuuagggcu aucuugcugu uccagcgcaa acuacucccc      720

gugccuguag ggaagugcuu ucuggaacca aaccagcccc ggguugcggc agcccuuccu      780

ucggcucaaa gauucaggcu gaugcaggag uugaaucacc uccgcgugau gacccuggcc      840

gauaaacgag aacggccauu aucauuucag gagcguaacg accugcucgc acaacuuguc      900

gccagaccga aguguggauu cgacaugcug aggaaaauug uguuuggcgc uaauaaggaa      960

gccuaccgcu ucacaaucga gagcgagcgg agaaaggaau ugaaaggcug cgauaccgca     1020

gcgaagcuag cuaaagugaa cgcccugggg acuagguggc aggcacucag ucuggacgag     1080

caggaucgac uugucugucu guugcucgac ggagaaaaug augccguucu ggcugacgcc     1140

uuacgcgagc auuauggucu gacagaugca cagauugaca cccuacucgg ccuguccuuc     1200

gaagaugggc acaugcggcu uggaagaucu gcuuugcuga gggugcucga cgcccuggag     1260

agcggccgug augagcaagg ucuuccccug uccuacgaca aggcgguagu ggccgcuggg     1320

uauccugcac acacggccga cuuggaaaac ggcgagcgcg augcucuccc auacuacgga     1380

gaacuguuau ggcgguauac ucaggacgcc cccaccgcga aaaaugaugc agagagaaag     1440

uuuggcaaaa ucgccaaccc uacaguccau auagggcuga accagcuaag gaagcucgug     1500

aaugcucuga uucagcgaua cggaaagcca gcccaaaucg uuguggaacu ugcacggaac     1560

uugaaagcug gucuggagga gaaggaacgc auuaaaaagc agcagacugc caaucucgag     1620

agaaacgaaa ggauccggca gaaacugcaa gacgcaggcg uccccgauaa ucgagagaac     1680

agauuacgca ugaggcuguu cgaggaacuu gggcagggaa auggcuuggg uaccccgugc     1740

aucuauucag ggcgucagau uagucuccag cggcuguuua gcaacgacgu gcaaguagau     1800

cacauacuac cuuucucuaa gacacuggac gauuccuuug ccaauaaggu ucucgcucag     1860

caugacgcga acagauacaa aggcaauagg ggacccuucg aggccuuugg cgcaaaccgc     1920

gauggguaug cuugggacga uauccgagcc agagccgcug ugcugccacg gaauaagagg     1980

aaccgcuucg cagaaaccgc caugcaggac uggcuucaca augagacgga cuuuuuggcg     2040

cgucaacuga cugauacagc uuacuuauca agagucgccc ggcaguaucu caccgcaauu     2100

uguagcaaag acgaugugua cgucaguccu ggaaggcuga cugccaugcu ucgcgcuaag     2160

uggggucuga accgaguguu ggacggcguu auggaagaac aggggagacc cgcagugaaa     2220

aaccgggaug accacaggca ucacgccauc gaugcgguag ucauaggagc uacagaccgc     2280

gccaugcucc agcaaguggc aacccuggcc gcuagagcca gggagcagga ugcagaacgg     2340

cuaauuggcg acaugccaac gcccuggccu aauuuccugg aggauguucg ugcugccgug     2400

gcgcgcugcg ucgugucuca uaagccagac cacgguccgg aagggggccu ccauaacgau     2460

accgccuacg gaaucguagc aggccccuuu gaggacgggc gauauagagu gaggcaccgg     2520

guuucccugu ucgaccuuaa gccuggagau uugagcaaug uccgcuguga cgcuccacug     2580

caggccgaau uagagcccau cuuugagcag gaugacgcua gagcaaggga aguggcccuc     2640

acagcccugg cugagcggua ccgacaaaga aaaguguggc uugaggagcu gaugucgguc     2700

cuaccuauuc gcccaagggg cgaagauggu aagacucucc ccgacucugc gccguauaaa     2760

gcauacaagg gggauuccaa cuauugcuac gagcuguuca ucaaugagcg gggacguugg     2820

gacggcgaau ugauuucaac cuucagagcc aaccaggcug ccuauaggcg cuuucggaau     2880

gauccugcac gauucagaag guacacagcc gguggccgcc cacuguuaau gcggcucugu     2940

auaaacgacu acaucgcugu ugggacugcg gccgagagaa ccauuuuucg uguggugaaa     3000

augagugaaa auaagaucac acuggcagag cacuucgagg gaggcacccu uaagcagagg     3060

gaugcugaca aagacgaucc cuuuaaguau cugacuaaaa gcccuggggc cuugcgggac     3120

cucggagcac gccgaaucuu cguagaucug auuggcagag uccuagaccc cgguauaaag     3180

ggggauuga                                                             3189


<210>  2664
<211>  3189
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2664
augaaccuga auuacauccu cggacuugac cuggggauug ccagcguggg augggcuguc       60

guugagauag augaacagga gaacccccaa agauugaucg acgugggugu aaggaccuuc      120

gaucgggcag aaguguuaaa gacaggcgag ucccuggccc uagcgcgccg acucgcucgu      180

ucuucaagaa ggcuggucaa acggcgcgcc gaaagaauua agaaagcaaa gaggcuucug      240

aaagccgaga auuugcuccu gagugcugac gagauccugc cuaacgaagc auggcagcuc      300

cgggugaagg gguuagauca gaagcuggag cgccaggaau gggccgcugu ucuuuugcau      360

cugcuaaaac accgaggaua ucucagccaa agaaagaaug aguccaaauc ugagaacaag      420

gaacugggcg cccuucuguc ggguguggcg auuaaucacc agaucuugca gucagcagag      480

uacaggacuc cagccgaaau agcugucaac aaauuucagg ccgaggaagg gcauauccgg      540

aaucaacgug gagcauauac gcacaccuuc agccgccucg accuguuagc ugagauggag      600

cugcucuuuc agagacaggc cacacuuggc aacccgcaua ccaguacuaa gcuguuggaa      660

aaucuaacag cgcugcucau guggcagaag cccgcccugg caggcgaggc uauucuuaaa      720

augcugggga agugcaccuu cgaaccuacu gaguacaaag ccgcuaagaa cuccuauucu      780

gccgaaaggu uugugugguu gacaaaacuc aauaaccugc ggaucuuaga gcaaggagca      840

gagcgagccc ugaccgauaa ugaacgcuuc gcucuacucg accagccaua cgagaaggcg      900

aaacugacgu augcacaggu aagaacucuu uugggucuga gcgaugaagc cauuuuuaag      960

ggccucaggu accaagggga ggacaagaaa gcuguugaaa ccaagacaac ucugauggag     1020

gugaaagccu accaccagau ccggaaggca cuugagggac uggauuugaa agccgaaugg     1080

aacgagcuca agaacaaucc cgcucuguua gacgaaauag gcaccgccuu cucccuguau     1140

aagacagaug acgacauuca ccgcuaccua gaugguaaac ucucagagag uguccugaac     1200

gcacuuuugg agaaucugaa cuuugacaag uucauccagc ucagccugaa agcguuacag     1260

caaauucugc cucuuauguu gcaggggcag agauaugaug aagcuguguc ugccaucuac     1320

ggagaccauu auggcaagaa auccgaugag aucgacaggc uccugccaaa uauucccacc     1380

gaugaaauac guaacccugu gguccuacgg acgcugacuc aggcacgaaa gguuaucaau     1440

gccgugguaa gacucuacgg cucaccagcu aggauucaca ucgagacagg gcgcgaagug     1500

ggaaagagcu aucaagaccg gaaaaagcug gagaaacagc aggaggaaaa cagaaagcag     1560

agggagaaug ccgucaaaaa guuuaaggaa uacuucccga acuuuguggg ugagcccaaa     1620

ggcaaggaua uacuuaaaau gcgcuuguac gaacugcaac aggcaaagug uuuauauagu     1680

gggaaaucuc ucgagcugua ccgacuucug gagaagggau auguugaagu ggaccaugcu     1740

uugccuuucu cccggaccug ggaugacagc uucaauaaca aaguccucgu gcuggccaau     1800

gagaaccaga auaagggcaa cgcgacucca uacgaauggc uagacgguaa gaacaauucg     1860

gagggguggc agcacuuugu agcccguguu caaacaucug gcuucuccua ugcuaaaaag     1920

cagagagugc ugauucagga acucgaugag aaaucauuua ucgagaggaa ccugaaugac     1980

acccgcuacg ucgcacgguu ccuuugcaac cacaucgccg auaauauguu gcugacggga     2040

aagggcaaaa gaaagguguu ugcuaguaac gggcaaauua ccgccuuacu caggggaugu     2100

uggggucugc agaagguccg agaaaauaac gaccgccauc acgcacuuga ugcggugguu     2160

guggccugca gcacaaaauc caugcaggac aagaucacua aauucguaaa guauaaagag     2220

ggccggcugu uuaaggauaa gaaagacggg gucuacaagg gagaaagaau uaauagggag     2280

accggcgaga uaaucucucu acauuucccc acaccuugga aauuuuucaa ggaaaacgug     2340

gagauucgua ucuuuagcga aaauccaaaa cucgagcugg aaaaccgcuu gcccgauuac     2400

ccgcaguaua aucacgaguu cguucaaccu cuguuugugu cacggaugcc cacuagaaag     2460

augaccgguc aggggcauau ggagacaguc aagagugcua aaagguuaga caagggccuc     2520

uccgugcuga aaguaccacu uacccagcug aaguugagcg aucucgaacg aauggugaac     2580

cgggagcgcg aaauggcccu guacgaggca cuaaaagaau gucuggagca guucggaaau     2640

gacccugcua aggccuucgc agagcccuuu uauaaaaagg gcggggcccu uguuaaggcu     2700

gucagagugg aacaaacuca gaaaucugga gugcucguca ggaacgguaa cggcguugcg     2760

gauaaugcca cggggucaau cgugcgggug gacguauuca caaagggagg caaauacuuu     2820

cuggucccaa uuuauaccug gcaaguggca aaggguauau ugcccaacaa agcuguuauc     2880

caggggaagg acgagaguga augggaggaa auggaugaga augccguguu ccaguuuagc     2940

augcacaaga acgaccugau uaaagucaag acuaaaaagg gcgagaucuu cggauacuuu     3000

aauggcuuag aucgagccac cggguccauu gaaaucgagg aacacgaccu cgagaaaucu     3060

aagggaaaga acggcucgca uagaggucug ggggugaaaa cagcuuucuu ucuugagaag     3120

uaucagguug augaacuggg caaaaauaua cgcccuugca ggccaaccaa gcgucagcac     3180

gugcgguga                                                             3189


<210>  2665
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2665
auggagaacc acaucaauua cagacugggc cucgacauug ggauagccag cgugggaugg       60

uccgucauca accuugauaa gaaaaggauu gaagaccugg guguucggau cuucaaugcu      120

gcagagcauc ccaaggaugg caguucauug gccuuaccuc gccgaauugc gcgugggaga      180

aggcggcugc uacgcagaaa ggcuuauagg gugaagcggg uacgcaaacu caucguggac      240

uacaacauac ugaccaagga ucagcuuaau gaccuguuua agcaaaaaug ccagaacagu      300

gucugggaaa uccgagugaa ggccuuggau aauaaaguua caaacgagga augggcaaga      360

cuccugauua aucuguguaa gaggcgggga uucaaaagca accguaagaa ugaggccgac      420

gauaaggaga cuaaaaaggu gaucaacucc auuaaaucua auaucaaggg caugaaagaa      480

ucggguucac gcacgauagg ggaguauauu uacacccaga cacucagcag uggagacauc      540

uccaagucuu uuagaaacaa agauggccag uauaauaacu gcgucagcag gaagaugauu      600

gaagaggaga ucaagaucuu auucaaaaag caacgggaac ugggcuguga guuugcuucc      660

gaagagauug aagagaaaua ccuugcaaua uucaacucac agcgaccauu uaguaaguuc      720

gaggacuugg agaaaauggu gggguuuugc acuuucgaaa aggccagcaa ggaacgcaga      780

gcuccgaaau guucuaucuc cgccgaggaa uuuguacugu augauaauau gaacaagcua      840

ucaauucuca auaaaggaga caccaggaag cugacaaacg aggagcggaa aaucauaguu      900

gaaaaugcgu ucaagaacaa ggagauuaaa uacagcaaac uucgcaaaau ccugaauuug      960

aguaacgacg aaagauuuuc uacccucucc uacagcaaua agacugagcu gucgaaaaca     1020

gaaaacucua aguucguggc auuaaagggu uaucacgaga ucaaauccgc cauugagaag     1080

ucauucggca aagauaccug gaauaggguc aagaacaauc guacgaugcu gaacgacauc     1140

gcuuacgugc ucaguauugc caaaacugau gaagacauaa ucaagcagcu uagccugcgg     1200

aauauucccc gagagaucau cgaugcagug uuggggcuau ccuuuucuaa auucagcaac     1260

cugucaauua aggcuauaga aaagauccuc ccuuaucuga aagacggaca gcaauacaac     1320

gaggccugca agagugcggg cuaugauuuu aaagccaccu acaaugguga gaaguccuau     1380

aaacuuccac ugauugacau cgaugaaauu gucaaccccg uugugaauag agcauugagc     1440

cagacaagga agguaaucaa cgcugugaua gaccgcuacg ggucuccuau cggaauuaau     1500

aucgagcucg cccgggaucu ggcuaagaac uucaaagaca gaucaaagau uaaaaaggaa     1560

caggaggaaa auagggccaa caaagagaag aucaagaaug aguuaaaaau gcugaugaac     1620

aaggaaccaa cuucuagcga gauacuaaaa uaccgccucu ggaaggaaca gcgagaggaa     1680

ugugcauauu cccaagagcg gauuuacauc gaggaccugu uuaaagucgg ccaguaugaa     1740

auugaucaua ucauacccuu cucucguucg uuugacgaua gccuuaauaa caagguguug     1800

guuaaggccg cugagaauca gagaaaagcg aacaggaucc cuuacgaaua uuucggcucc     1860

gacgaggauc gcugguaccg guuugaagug ugggucgagc aaucaagucu gucuuucaag     1920

aaaaagaaua accuccugaa aaagagauuu agcagggagg acuccauuga guucaaagau     1980

cgaaaccuug uggacaccca guauaucuca cgcuacauua gcaauuuuau caagucucgg     2040

cugauauuca aggaauccga uaguaaacag aagguaauua caaucaacgg gagagcaacc     2100

agcuacuuga gggccaaaug gggacuccug aagguucgug aaaaugguga caaacaccau     2160

gcuuuagaug ccacggugau cgcagucgcc acucagggca uggugcaaga gauuucuaag     2220

uauuccaagg cucacgagcu gaaauacauc cgccaggggg acgaguucau ugauauagaa     2280

acaggagaaa ucgucaaggu gaaagaccac aagcaucuau caaaagaccg gcucccagag     2340

ccguggcugg gcuuuaguag cgaacuuaga uugaggcugu cggagaaccc cauugaggaa     2400

cucaagaagu ccccuaucuc uaccuaugau gaggaguuca uucgaaaaac uguucggcca     2460

aucuuuguga gccgcguacc cuacagaaag gucacaggua ggcuguucga agaaaccgug     2520

uauucaccua aagccuuuau agacgagggg uucaucgcaa agaaaaaguu acaggaucug     2580

acgcuuaagg acuuugagaa uauuuacaac uauaauagug auaaaaaguu guacgacgcg     2640

cuccgggaac gacuggagga auacggcgga aacgcuaaaa aggccuucgc agaggaauuu     2700

agaaaaccaa ccaagacagg caagcuaggg cccaucguua aauccauuaa ggugaaaacu     2760

aagaucccgu ucaaagaugg aauagaccug aaugguggca aggucaaaaa ggaugggcuc     2820

gugcgcauug acaucuauga gaaggaggga aaauacuaug uagugccugu uuacaggcug     2880

gauauccagc aaggcaacau ucccaagaaa gccgcugucg ccaauaagga agagaaagac     2940

uggauacuua uggaugaauc uuauaacuuu aaguucagcu uguacaagaa ugaccugauc     3000

uauauuaaau acgacuccaa gggugaguac uuuggguauu acgauggcuu cgaccgucac     3060

accggagcaa ucacauuaga gcggcaugau aacucagaaa gauauagggg cauugggauc     3120

aaaacuggag ugaaggagau auucaaauac aauguggacg uccucggucg cuauuacaag     3180

guugcuaagu ga                                                         3192


<210>  2666
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2666
auggagaagu uccacuacgu gcugggccuc gaccuuggga ucgccagcgu cggaugggcu       60

gcaauugaaa uagauaaaga gaccgaaaca uccaucgguc uguuggacug cggcguuaga      120

acuuuugaga gggccgaagu gcccaagacg ggggauucuu uagcgaaagc ucggcgcgag      180

gcccgaucaa cccguagacu gauuaggcgg cgcagucaua gacuacucag gcugaagcgg      240

cuucugaaac gcgagaucuu ccgacagccu gaaacauuua aggacuugcc aauuaacgca      300

uggcagcuca gaguaaaggg acuggauagc aggcugaaug aguaugaaug ggccgcugug      360

cucuuacacc uggucaaaca ucggggcuac cuuucccagc guaagucuga gaugucggag      420

acugacucaa aaagcgaaau gggucgcuug cuggcagggg uggccgagaa ccaccagcua      480

cuccagcaag aacaguauag aaccccggcu gagcuggccc uuaagaaauu cguuaagcau      540

uuuaggaaua agggaggcga uuacgcgcac acauucaacc ggcuggacuu gcaggcagaa      600

cuccaccugu uauuucagaa acaacgagag cugggcaauc ccuucaccag uccugagcuc      660

gaacgccagg uggaugaccu ucugaugacu cagagauccg ccuugcaggg ggaugcuauc      720

cuaaagaugc ugggacauug ugguuuugag ccagaacaau ucaaagccgc aaagaacaca      780

uuuucugcug agagguucau auggcucacc aaacugaaua accuucggau ccaggaccag      840

ggcaaggaac gcgcccugac ggcggaugag agaacuaaau ugcucgacga gcccuauaag      900

aagucaaaac ugaccuacgc ccaagucagg aaguuacugu cccuaccuca gacagcaauu      960

uuuaaagggc uccguuauga ccuggaacac gauaagaaag cugagaauag cacucuuaug     1020

gaaaugaaga guuaccauaa cauccggcag accuuggaga agucuggacu gaaaacagaa     1080

uggcagucua uugccaccca accagagauc cucgacgcua uaggcacggc cuucuccauu     1140

uacaagacug augaggacau cucacacgaa cugaaaacau gccgacuucc cgagaaugug     1200

cugaacgaau ugcucaagaa uauuaacuuc gaugguuuua uccagcugag cuuaaccgca     1260

cugagaaaaa uccuaccucu cauggagcag ggguauaggu acgacgaggc cuguacucag     1320

auuuauggaa aucaccauag uggcucucug caacaggaau ccaagcaguu ccuuccacac     1380

auaccgaucg augacguacg caaccccguu guguuucgga cauugaccca ggcuagaaag     1440

gucgugaacg cgauuaucag gcgcuacggc agcccugcac gagugcauau agagauggcc     1500

cgggaacugg ggaaaucgaa gucugaucgu gacagaauug agaaacaaca gcagaagaau     1560

aaaaaggaaa gggagaacgc ugucgccaag uucaaagagg auuuuccaga cuucguugga     1620

gaaccccgcg guaaggacau ccucaaaaug cggcuguaug agcagcaaca cggcaagugc     1680

uuauacuccg ggcaugauau cgacauuaau agacugaacg aaaaaggaua uguggagauc     1740

gaucacgcac uuccuuuuuc aaggacgugg gacgauaguc agaauaacaa gguauuggug     1800

cucggcagcg aaaaucagaa caaacgaaau caaaccccag acgaguaccu ggauggugcc     1860

aacaauuccc agcgcuggcu agaguuccag gcucgggucc agacauguca cuuuucuuac     1920

gggaagaagc aaaggauuca gcuggccaaa cucgacgaug aaacugagaa gggcuuccug     1980

gaaagaaacc uuaaugacac ccguuauaua gcacgcuuua ugugccaguu cgugcaggag     2040

aacuuguacc ugacaggaaa aggcaagcgg uuaguuuuug cgagcaaugg gggaaugacu     2100

gcuacccuca gaaaccugug gggucuuagg aaagugcgag aagauaacga ccggcaucac     2160

gcccuggaug caaucgucgu ggccuguuca acagcuagua ugcaacagaa gauuaccaag     2220

gccuuccagc gccaugaguc caucgaguau guagacacug aaacgggcga gguuaaauuc     2280

agaauccccc agccguggga cuuuuucagg caagaaguga ugauucgggu cuuuagcgau     2340

cagccuugcg aggacuuggu ggagaagcuc ucugcacgac ccgaagcucu gcacgauaau     2400

gucacaccac uauucguguc aagagccccu aaccgcaaaa ugagugggca gggacaccug     2460

gagaccauaa agagcgcgaa aaggcucucc gaagagaaua gcaugguuaa gaagccccug     2520

acuacccuua aauugaagga caucccagaa auugugggcu accccucgcg ugagccucaa     2580

cuguaugccg cuuuaaaaac acggcucgag acccaugaug acgauccaau caaggcauuu     2640

gccaaaccgu ucuacaagcc caacaagaau ggugaacugg gggcucuugu aagaagcguc     2700

agggugaaag gcguucagaa cacuggagug augguccacg acggcaaggg gauugccgau     2760

aaugcaacaa uggugcgcgu agacguguau acgaaagcgg gaaagaacua ccugguuccu     2820

gucuacgugu ggcaaguggc ccaggguauc cuaccaaauc gagcugucac cuccggcaaa     2880

ucagaggccg auugggaccu cauagaugaa aguuuugagu ucaaguuuuc ucugagcaga     2940

ggggacuugg uugaaaugau cuccaacaaa ggacggauuu ucggcuauua caauggucug     3000

gacagggcaa acgggucaau cggcauucgc gagcaugauu uagaaaagag caagggaaaa     3060

gacggcgugc accguguggg gguaaagacu gcuacagccu uuaauaaaua ucaugucgau     3120

ccccucggaa aggagaucca cagauguucu uccgagccuc ggccaacccu gaaaauaaag     3180

aguaagaaau ga                                                         3192


<210>  2667
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2667
auggagaagg acuacauccu gggccucgau auugggauag gaagcguggg uuauggccuu       60

aucgacuacg auaccaaauc cauuaucgac gccgggguca gacuguuccc cgaagcuaac      120

gcagauaaua acuugggaag gcgggccaag cgcggcgcgc gacguuuaaa aagaaggcgg      180

auucaccgcc uggagagagu uaagucucua cucucagaau auaaaaucau aaguggucug      240

gcuccuacaa auaaccagcc auacaauauc agggugaagg ggcuuacuga gcaacugacg      300

aaggacgaau uggccguagc acuccugcau auugccaaac ggcgcggaau ccacaacgug      360

gaugucgcug cagacaagga ggagaccgcc agcgauuccc ugucuacaaa agaccagauu      420

aauaagaacg cuaaauuucu cgaaucgcga uaugugugcg aguuacagaa ggaaagacug      480

gagaaugagg gccauguuag gggcguggaa aaccgguucc uuacuaagga uaucguccgu      540

gaggccaaaa agauaauuga cacccagaug caauacuauc cggaaaucga ugagacauuu      600

aaagaaaagu acauuucauu gguggagacc cgcagagagu acuaugaagg gcccggaaaa      660

gguagcccuu acggcuggga cgcggacgua aagaaauggu aucagcugau gauggggcac      720

uguacuuacu ucccaguuga guuuaggagu gugaaguaug cauacacagc cgaucuauau      780

aaugcucuca acgaccugaa uaaccuuacc aucgcccggg augacaaucc caagcuggaa      840

uaccaugaga aauaccacau cauugaaaac gucuucaagc agaaacgaaa cccuacguug      900

aagcagauag caaaagagau cggagugaau gauauuaaca ucuccggcua ucgcgugacu      960

aagucuggua agccacaauu uaccagcuuc aaacucuuuc acgaccugaa gaaagucguu     1020

aaggaucaug cuauauuaga cgauauugac cugcucaauc agaucgccga gauccuuaca     1080

auuuaccagg auaaagacuc caucguggcg gaacuggggc aguuggagua ucuaauguca     1140

gaagccgaca agcaaaguau uagcgagcug acuggauaca ccggcacaca cucucucucc     1200

cugaagugca ugaacaugau aaucgaugaa cuuuggcauu caagcaugaa ucagauggag     1260

guauucaccu aucugaacau gagacccaaa aaguacgagu ugaaaggcua ucagaggauu     1320

ccuacggaca ugaucgauga cgcaauccuc aguccagugg ucaagcgguc uuuuaaacaa     1380

gcuauugggg ugguuaaugc cauaaucaag aaguacggac ugccgaaaga uauuaucauu     1440

gaauuagcuc gcgaguccaa cagcgccgaa aagucgagau accugagggc aauccagaaa     1500

aagaaugaga aaacucguga gcggauagaa gccaucauua aggaguaugg uaacgaaaau     1560

gcuaaaggcc uagugcagaa gaucaagcuc cacgacgcgc aggaggggaa augucuguac     1620

ucucuuaagg auauucccuu ggaagaccug cuccgaaacc cuaauaacua ugauaucgac     1680

cacauaauuc caagauccgu cucauucgau gacaguaugc auaauaaagu gcugguaagg     1740

cgcgagcaaa acgcaaagaa aaacaaucag acacccuacc aguaucuuac cagcggauac     1800

gccgauauca aguauuccgu uuuuaagcag cacgugcuga acuuggcuga gaauaaagac     1860

cggaugacua agaaaaagag agaauaccuc cuggaggaaa ggaacauuaa uaaauacgac     1920

guccaaaagg aguucaucaa ccgcaauuua guggauacac gauauaccac gcgggaacug     1980

accacacuac ucaagacuua cuucaccaua aacaaucugg acgucaaagu gaagacaauc     2040

aacggcucuu uuacugauuu ccuucguaaa agaugggguu uuaagaaaaa uagggacgag     2100

ggguauaagc aucacgccga ggaugcauug auuaucgcca acgcugacua ccuguucaag     2160

gaacauaaac uccugaagga gauuaaagau guuagcgacu uagccggcga ugaacgcaau     2220

ucaaacguga aggacgagga ucaguaugaa gagguauuug gaggcuacuu caaaaucgag     2280

gacauaaaga aauauaagau uaagaaauuu agucaccggg ucgauaagaa accuaauaga     2340

cagcugauca acgacaccau cuacuccaca agggugaagg acgauaaacg auaccuuauu     2400

aacaccuuga agaaucucua ugacaagagc aacggggauc ugaaagaacg caugcagaag     2460

gacccagagu cucuacugau guaccaccau gauccccaaa cuuucgaaaa acucaagauc     2520

guuaugucac aguaugagaa ugagaaaaac ccgcuggcaa aguacuuuga agagacggga     2580

caguaucuua caaaguacgc gaaacacgac aaugguccug cuauucauaa gauaaaauau     2640

uacggcaaca aguuggugga acaccuggau aucaccaaaa auuaccacaa cccccagaau     2700

aaggucgugc aauuaaguca aaagagcuuc cgguuugacg uauaucagac ugauaaaggg     2760

uacaaguuca uuuccaucgc cuaucucacc cugaaaaacg agaagaauua cuaugcaauu     2820

ucucaagaaa aauacgacca gcuuaagucg gagaagaaaa ucagcaacaa ugccguguuu     2880

auaggauccu ucuauacauc agauaucauu gagaucaaca augaaaaguu cagaguuauu     2940

ggcgucaaca gugacaaaaa caaucugauc gagguggaca ggauagauau ucgucagaag     3000

gaauuuaucg aguuggaaga ggagaaacag gugaacuaca ugaacucuga cagcuaccuc     3060

aagaauaauc gcaucaaagu caccauuggu cggaagacua caaacauaga aaaguuccau     3120

acggauaucc uggggaauau guauaaaucc aagagaccaa aagcuccuca gcuaguuuuu     3180

aagaaaggcu ga                                                         3192


<210>  2668
<211>  3195
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2668
augagcgagu gcgugaccag aaggauccug ggcauugacc ucgggauagc cuccugugga       60

ugggguguca ucgaaguugg cgaggcuucu gggucaauua ucgcaagugg agugcggugc      120

uucgaugcgc cccuuauuga caagacaggc gaaccuaaaa gcgccacucg ccgaacggcu      180

cguggucaga gaaggaucau acggcgcaga aggcaacgga ugaacgccgu acgccgacug      240

uuggcagagu uugggguguu aaccggaaga uccccagaug cccugcacca ggcucuacuc      300

aggcugucuc agucggucgc aggcucacag gugacaccgu ggacucuucg ggccgcugcc      360

caugaacgua agcugaccaa ugacgaguug gcgguugugc ucggccacau cgcacgccau      420

agaggguuca ggagcaacag uaaaaaugau ggaggugcca acgcugccga cgagacaucc      480

aagaugaaaa aggcaaugga aaccacucgg gagggccugg cucgauacca cucuuuuggg      540

gccaugauug cgagcgaucc caaguucgcc gaccgcaaaa gaaauaggga uaaggacuau      600

ucccauacag caaaacgguc agaucuggaa gacgaggucc gcaccaucuu uagaagucaa      660

acgagguucg gaagccucgu ggcuucugag aaguuauccc aggccuuugc ugaugccgca      720

uucuuucagc guccucugca ggacucagaa gacaugguag gcagcugucc auucgagccc      780

ggucaaaaac ggacugccag acgagcuccu aguuuugaac uuuucagguu uuugucucgc      840

cuggcgaacc uaaagcucac cguugggcgg gcaccagaga gaaggcugac acccgaugaa      900

auugcccuug cugccaaggg auucggcgag acuaaaaagu ccaucaccuu caaaagccug      960

cgcgaggcau uggaccucga uccuaaugcc cgauuuucgg gcguggcuaa ggaaaaagag     1020

ucuacacugg acgucgccgc acggaccggg ggagcggcuu acgguacgaa gacuuuaaaa     1080

gaugcccugg gcgacgcacc auggcguucc cucucaagaa ugccggaaaa acuugauagg     1140

auagccgaga uucugaguuu ccgcgaagac augaaggcua uccggaacgg guuggaggag     1200

gugggacuag auggccuggu ggucgacgcc cucaugcagg caacagcuaa uggugauuuu     1260

aaagacuuca ccagagccgc gcacauuagc gcccuggcug caaggaacau cauccccggg     1320

cuucgagaag gccugguuua uuccgacgcc ugcacucgcg ugggauacga ucacgcugcc     1380

cggccugcag uaccauuguc ucagauuggc agccccguga caagaaaggc gcucucagag     1440

gcccugaaac aggucagggc uguggcccgu gaauaugggc cuauagacua cuuucauauc     1500

gaguuagcac gcaguauugg aaaguccgcu gaggaacgga aaaagcugac cgaugguauc     1560

gaggccagaa auguugaaaa ggagaaaagg cgaaaggaag cagccgagca ccuaggccgg     1620

gcuccaagcg acgaugagcu ccugcgcuau gaacuugcga aagagcaaaa cuucaagugu     1680

auauacucug gggaccccau ugauccggcc ggaaucucag caaaugacac gagauaccag     1740

guggaucaua ucuugccuug gaguagguuu ggcgacgaua gcuaugucaa caaaacccug     1800

ugcacagcuc gguccaauca gaacaagcga gguagaacuc ccuucgaaug guuugacgcc     1860

gauaagaccg aggccgaaug gauggaguac ucugcucgcg uggaggaccu caaagaagua     1920

aaggggagga aaaagcguaa cuauucgauu aaagacgcag ccagcguuga ggauaaguuc     1980

aaggcgcgga aucugacaga cacucaaugg gcuaccagac uucuggccga ugaauugaaa     2040

aggauguuuc caccucgcga gugugaacga guggucacag ugagagcaga cggcggaaac     2100

gauggccucu ccaucgucga ggagcggagg guguucaccc gccccggggc cauuacuuca     2160

aagcugcgua gagcuugggg auuagaaggu cugaaaaagc aggacggcaa acggguugag     2220

gaugacaggc accacgcagu ggaugcccua guacucgcgg cuacgacaga aagucugcuu     2280

aaucgcuuga ccgucgaggu gcagcagcga gaaagagagg ggcggcaaga cgauauauuu     2340

cauugcucuc agccauggcc cggauucagg guugacgugc agcgcacugu cuacggcagc     2400

gagaccaugc cugguaucuu cguguccaga gccgaaaggc ggcgugcacg cgggaaggcc     2460

cacgaugcua caguaaaaca gauucgagac aucgacggcg agagaaucgu guuugaaagg     2520

aagccaauug agaagcugac cgauaaagac cucgagcgga uaccgguucc cgaaccuuau     2580

ggaaaggccg cagauccaaa gaagcugcgc gacgaguuag ucgaaaaccu gagagcuugg     2640

aucgccgcgg gcaaacccaa ggauaagccu ccaaggucac ccaaagggga cauuauccgg     2700

aaggugcgaa uugagacuaa agauaaggug gccgucgaaa ucaauggagg cacaguugac     2760

agaggugaua uggcacgcgu ggacguguuc aggaaaaaga acaagaaagg gguaugggag     2820

uuuuacguca uaccgaucua uccucaucaa auuguggcua gcgcccuucc acccaaucgg     2880

gcuguuaucg cauacaaggc cgagucugaa uggacggcca uugauggaug uuucgaguuu     2940

gcuugguccu ugaacccuau gaguuaccuc gaacugguga aaagcaaugg cgagcuaauc     3000

gaagguuauu uccguucuau ggacagaacc acuggcgcga uaaaccuguc ccccauguca     3060

acaaauagug agaccauuag gagcaucggg gucaagacac ucucguccuu ucgcaaauuc     3120

acuguggacc ggcugggacg aaaguuugag auuccaagag aaguuaggac cuggcgcggc     3180

gaggcaugca cguga                                                      3195


<210>  2669
<211>  3195
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2669
augaagagaa ccagccugag ggccuaccgg cucggcgugg accuuggggc uaacucccug       60

ggaugguucg ucguuugguu ggaugaccac ggucagcccg agggcuuagg gccuggaggc      120

gugcgcaucu uuccagaugg ucgaaauccg caaucuaaac agucaaacgc agccgggcgu      180

agacuggcga ggagugcucg gcgcagaagg gaccgguauc uacagcgccg aggaaagcuc      240

augggccugc uuguaaaaca uggccugaug cccgccgaug aaccugcaag aaagagguug      300

gagugccucg acccauacgg gcugcgggcc aaagcucugg augaagugcu ccccuuacac      360

caugucggac gugcacuguu ccaccuuaau cagcgcagag guuuguuugc caacagggcu      420

auugagcaag gcgacaagga ugccagcgcg auaaaggcag ccgcugggcg gcugcagaca      480

uccaugcagg ccuguggagc acgaacucua ggcgaguucc ucaaucgcag acaucagcug      540

agggcuacgg ugcgggcccg cucuccuguu gguggggacg ugcaagcgag auaugaguuu      600

uacccaacca gggccauggu cgaugcagag uucgaagcua ucugggccgc ucaggcaccc      660

caccacccua caaugacugc cgaggcccau gacaccauuc gugaagcuau cuuuucgcag      720

cgggcgauga aacgaccauc aauuggaaag ugcagccuug auccggcaac aagucaggac      780

gacguggaug gcuucagaug ugccuggucc cacccccugg cucaaagguu ucgcaucugg      840

caggacguac ggaacuuggc cguuguggag accggcccua cuucuagcag acucgggaaa      900

gaggaucagg acaaggucgc aagggcccug uuacaaacag aucagcuguc cuucgacgaa      960

auacgcggac uccuuggucu gccaucagau gcucgauuua auuuggagag ugaccggcgu     1020

gaucaucuaa aaggcgacgc caccggggca auccugagcg cgagaaggca cuucggaccc     1080

gcuuggcacg aucgcucucu cgaccggcag auugacaucg uggcccugcu ugaauccgca     1140

cuggaugagg ccgcuauuau cgccucauug ggcacgacuc auagccucga cgaagcagcu     1200

gcccagagag cgcugagugc cuuacugccu gaugguuauu gcaggcuagg gcuccgagcu     1260

auaaagcgcg ugcugccacu uauggaggca ggccggaccu acgccgaggc ugccucugca     1320

gcgggauaug accacgccuu gcugcccggc gggaagcucu ccccgacagg auaccugccu     1380

uacuaugguc aauggcuuca gaacgauguc guuggcagcg acgaugaaag agacacuaau     1440

gagaggcguu gggggcgccu gcccaaccca accgugcaua uuggaaucgg ccaguugcgg     1500

agaguaguga augaacucau uagguggcac gguccucccg cugagaucac agucgaacug     1560

acccgagauu uaaaacuguc gccacggcgc cuagccgagc ucgagagaga acaggcagag     1620

aaccaaagga agaaugacaa acggacgucu cugcuucgaa aguuggggcu gcccgcuucc     1680

acucauaacc uccugaaauu aagacugugg gaugaacagg gcgacguggc cucagagugu     1740

ccuuacacag gagaagcaau cggccuugag cgcuugguua gugaugacgu ggacauugau     1800

caccucauac cauuuagcau cuccugggac gauucugccg cuaauaaggu cgugugcaug     1860

agguaugcga accgugagaa agggaaccgg accccguucg aagccuucgg acacagacag     1920

gguaggcccu acgacugggc agauauugcu gagcgcgccg cccgacugcc uagaggcaag     1980

cgguggaggu uugggccagg agcucgcgca caguucgaag aacuaggcga cuuucaagcc     2040

cgucugcuca augagacuag cuggcuggcg agaguagcua agcaguaucu ugccgcaguu     2100

acacaucccc accggaucca uguguugccu gguaggcuga ccgccuuacu ccgcgcuacg     2160

ugggaacuga acgaucuucu gccaggguca gacgaucgag cagccaaaag uagaaaggac     2220

caccggcacc augcgauaga ugcuuugguc gccgcacuca ccgaccaggc ccugcuaagg     2280

cgcauggcua augcccacga ugacacaaga aggaaaauug aggugcugcu ccccuggccg     2340

acuuuccgga ucgaccugga aacccgucuu aaggcaaugu uggucuccca uaaaccugau     2400

cacggccugc aggcucgcuu acaugaggac acagccuacg gaacugugga acacccagag     2460

accgaggaug gcgcgaaccu cguuuaucga aagacauuug uggacaucag cgaaaaggag     2520

auugauagaa ucagggaccg gcgccugaga gaucuuguaa gggcccacgu cgcaggggaa     2580

cggcaacagg gaaaaacccu gaaggcugcc gugcuaucuu ucgcucagcg aagagacauu     2640

gcaggccauc ccaaugguau acgccacguu aggcucacua aaucaaucaa gccugauuac     2700

cuggugccca uucgggacaa agccgggcgu aucuacaaga guuauaacgc cggagagaau     2760

gcuuuugucg auaucuugca agcggaaagc ggcagaugga uugcaagggc cacgacagug     2820

uuccaggcua accaggccaa ugagucccau gacgcaccag ccgcucagcc uauaaugcgc     2880

guauuuaagg gugacaugcu gcggaucgau cacgcgggcg ccgagaaauu cgugaagauu     2940

guucgauuau cucccucgaa caaucuccug uaccuugucg aacaucacca agcaggggug     3000

uuucagacca gacacgacga uccagaggac agcuucaggu ggcuguuugc uuccuucgau     3060

aaauugcgcg aauggaacgc cgagcucgug cggaucgaca cucugggaca gccguggaga     3120

cguaagaggg gccuagaaac cgggucagag gaugcaacac ggauuggaug gacccgcccu     3180

aaaaaguggc ccuga                                                      3195


<210>  2670
<211>  3198
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2670
augaacaagg acaucaauua cgcccugggc cucgauauug ggauaaccag cgugggaugg       60

gcugucauca accuugaccu gaauagaauu gaggauuugg guguuaggau cuucaacgca      120

gccgaaaauu uaaaagacgg cuccucucug gcgcuacccc ggcgccucgc ucgagggcgu      180

agaaggcugc uucggcgcaa ggccuauaga guggagaggg uacggaaacu gauuuugaag      240

aacaauaucc ucacaaaaga agaacuggaa ggacuguuua agaacaagga ggugauagau      300

gucugggagg cacgcgugaa aggccucgac uacaaguuau cacgagaaga gugggccaaa      360

auccugauua auuucugcaa gagaaggggu uuuaaaagua accggaagaa ugaagcuaag      420

gauaaagagg cagggcagau ccuuagcucc auuucuuaua acaucgagaa gaugaaagaa      480

acuaauucgc guacgauagg agaguacauu uauaacgaag uuaaaaauuc agaggacagc      540

uacgccccuu ugcgcaacaa auucggcgaa uauaagaugu gugugaguag agaugucauc      600

agggaggaga uucacacccu guuugaaaaa caacgggagc uaggcaacaa uuucgcuucc      660

gacgaaaucg aggauaagua cuuugaaauc uucaacucuc agcgaccauu uagcaauuuc      720

gaggaccucg agaagcuggu gggguuuugc acauucgaac gcaaaaagca uaaaagagcc      780

ccgaagcacu guauuuccuc agaggaauuu acucuuuacg agggaauaaa caaacugagu      840

aucauuaagg auggugaaaa gaggaaauug agcgaggagg aacggacccu cguaguuaau      900

gaggcguuca acaagaaaga aaucaaguau acaacccugc gcaaauuacu gggccucucu      960

gaggaggaac uuuucuccac ucugacauac ucaauagaca aggacauuag caagaccgag     1020

aauacgaaau uugugaguuu gaagggguau caugaaauca aaagagcaau cgagaaggga     1080

gucucuaaaa aggccuggca ggauauugaa aacaauagga accuacugaa ugacaucgcu     1140

uacgugcuca cucugggcaa gaccgaugac gagauugaga aacagcuucg ucugcggaac     1200

gugcccgaag aguuguucga aucccuccug gauaugagcu uuaauaaguu caacaauuua     1260

ucgauauaug cccuggagaa aauccuaccu uuuaugaagg aagguuacca auauaacgag     1320

gcaugcgaga aagcugggua caacuucaag gccauuuacg aaggaguccg aacaaagaag     1380

cucccaguua ucgagaucga cgaaauugug aaucccguag ugaacagagc gcuggcccag     1440

acuaggaagg ucauaaauuc ugugaucgau aaauauggcu ccccuguucg cauuaacauc     1500

gagcuugcac gggacuuggc uaagaauuuu aaagauagaa agcugauuga aaaagagcag     1560

aaggagaaca gggccaaucu cgacaagauc cgcgaaaacc ugcgagagcu uauggauaaa     1620

gaaccaaccg uggucgaggu gcugaaguac cgguuguggg agcagcaacg uggcgaaugu     1680

gcuuauacac agucacagau accccucgag agacuguuca guccugggua cugcgaaauc     1740

gaccacauua ucccauuuag cagguccuuc gacgauucuu uaagcaauaa aauucuggua     1800

cuaggaucag agaaccagcg caaggguaau cggaccccgu augaauacuu uggcgaggac     1860

agugagagau ggaacuccuu cgaaaucugg guuaaaggga gcuaucucaa uuacaagaag     1920

aaagccaacc ugcuuaagaa aaaguuuucu gaggaagaac aaagggauug gaaagcacga     1980

aauuugcagg acacgaagua cauaugucgc uauauuucaa acuucgugaa caaucggcug     2040

gaguucaagg agagugagaa caaacagaag gucaucacug ugaauggcag agccacagcu     2100

uaccucagga gccguugggg acugaacaaa guccgcgaag auggugacaa gcaucacgcg     2160

uuagaugcag ccgugguugg ggugaccacu caaggcaugg uacagaaaau uuccaaguau     2220

ucuaaagcuc acgagcugua cacaguccgg aagaaugacg aauuugugga uguugagacc     2280

ggagaaugcg uggacgucga ugaguauaag gagaucagaa aagacauacu ucccaggccu     2340

uggcgaggcu ucucggaaga guugaagcuc cggcugagcg auaacccacu agaggagcug     2400

aaaaaguccc ccaucgagac guacaaugaa gaguuuauua aagaaaacgu gaagccuauc     2460

uucguaucac gcgugccauu uagaaagauu agugggaaac ucuucaagga gaccaucuau     2520

ucugaaaggg ccuuuaaaga cggauacuuc guuagcaaga aaucccuggu cgaccuuaag     2580

cggggugauu uggacaauuu uuacaacuau gagugugaua agaaacugua cgacgcaaua     2640

ucagagcgaa ugaaggaguu caauuauaac ggcaaaaagg ccuuugaaag ccccuucaga     2700

aaaccgacaa agucugggaa ggaaggaccu guggugcgcu ccgucaaaau uaagagugcu     2760

guucccuuua aagauggcau cccaaugaau gaggguuuag uggccaagga agggauggug     2820

aggaucgaca uuuacgagaa agagggcaag uauuucguag ucccugugua ccguuaucag     2880

cucgcaaagg gaaucguucc caaacgggcg gcuauugcca agaaagauga acugaagugg     2940

ccaauaaugg accagacuua caacuucaaa uuuagcaucu acaagaauga ucuuauugag     3000

gugaaauaug acaagaaggg cgucuacuuc ggguauuacg auggauuuga cagaaacucu     3060

gcaucccuga ccaucgaaaa acaugauaac ucagagaggu aucgcaguau ugguuugaag     3120

gugggcguuc ucgaaaucaa uaaauacgag guggacguac uggggaacua ucacaagguc     3180

aaacuaggag gcaauuga                                                   3198


<210>  2671
<211>  3198
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2671
augaacaagg acaucaauua cgcccugggc cucgauauug ggauaaccag cgugggaugg       60

gcugucauca accuugaccu gaauagaauu gaggauuugg guguuaggau cuucaacgca      120

gccgaaaauc ccaaagacgg cuccucuuua gcgcugccuc ggcgccuagc ucgagggcgu      180

agaaggcucc ugcggcgcaa ggccuauaga guggagaggg uacggaaacu uauugugaag      240

aacaauaucc ugacaaaaga agaguuggaa ggacucuuua agaacaagga ggucauagau      300

gugugggagg cacgcguuaa aggccuggac uacaagcugu cacgagaaga gugggccaaa      360

auccucauua auuucugcaa gagaaggggu uuuaaaagua accggaagaa ugaaacuaag      420

gauaaagagg cugggcagau cuuaagcucc auuucuuaua acaucgagaa gaugaaagaa      480

acgaauagcc guaccauagg agaguacauu uauaacgaag ugaaaaauuc agacgauagc      540

uacgcaccac ugcgcaacaa auucggcgag uauaagaugu gugucaguag agacgugcuu      600

agggaagaga uccacacauu guuugagaaa caacgggaac ugggcaacaa uuucgccucc      660

gaugagauug aagacaagua cuuugagauc uucaacucuc agcgaccguu uagcaauuuc      720

gaagaucuag agaagcucgu aggguuuugc acuuucgagc gcaaaaagca uaaaagagcu      780

cccaagcacu guaucuccuc agaagaguuu acccuguacg aaggaauuaa caaacuuagu      840

auaaucaagg acggugagaa gaggaaacug agcgaagagg agcggacauu gguugugaau      900

gaagccuuca acaagaaaga gauuaaguau accacucucc gcaaacuguu aggccugucu      960

gaagacgaac ucuucuccac acuuaccuac ucaaucgaua aggacauaag caagacggag     1020

aauacuaaau uugucagucu gaagggguau caugaaauua aaagagcgau cgagaaggga     1080

gugucuaaaa aggcauggca ggauaucgaa aacaauagga acuugcuaaa ugacauugcc     1140

uacgugcuga cccucggcaa gacagaugac gagaucgaaa aacagcugcg ucuucggaac     1200

gucccugagg agcuguucga auccuugcuc gauaugagcu uuucgaaguu caauaaccug     1260

ucuauuuaug cuuuagagaa aauacugcca uuuaugaagg aagguuacca auauaaugag     1320

gccugcgaaa aagcagggua caacuucaag gcuaucuacg agggaguucg aacuaaaaag     1380

cuacccguga uugagaucga cgaaaucgua aacccugugg ucaauagagc ccucgcgcag     1440

accaggaagg ugauuaacuc cguuauagau aaauauggcu caccagugcg caucaauauu     1500

gagcuggccc gggaccuugc aaagaacuuu aaagauagaa aguugaucga aaaagagcag     1560

aaggaaaaua gggcuaaccu ggacaagauu cgcgagaauc uccgagagcu gauggacaaa     1620

gaacccacag ccgucgaggu gcuuaaguac cggcuguggg aacagcaacg uggcgagugu     1680

gcuuauaccc agagucagau cgccuuggag agacucuuca gcccugggua cugcgaaaua     1740

gaucacauca uuccauuuuc caggucuuuc gacgauagcc ugucaaacaa aaucuuagua     1800

cugggaagug agaaucagcg caaggguaac aaaauuccgu augaauacuu uggcgaggac     1860

uccgaacggu ggaauagcuu cgagaucugg guuaaggggu cuuaucuaaa cuacaagaaa     1920

aaggcaaauc uccugaaaaa gaaauuuacg gaggaagaac aaagagauug gaaggccagg     1980

aaccuucagg acacuaagua cauaugucga uauauuucaa auuucgugaa caaccgcuug     2040

gaguucaaag agagugaaaa uaagcagaaa gucaucacag ugaacggacg ggcuaccgcg     2100

uaccugagau cgaggugggg ccucaauaag guccgugagg auggugacaa acaucacgca     2160

cuggaugccg cugugguugg ggugacuaca caaggcaugg uacagaagau uuccaaauau     2220

ucuaaggccc acgaguuaua caccguccgc aagaacgacg aauuugugga uguugagacg     2280

ggagaaugcg uggacgucga ugaguauaaa gaaauccgga aggacauacu gcccagaccu     2340

uggaggggcu ucucggagga gcuuaaauug cgacucagcg acaauccacu ggaggagcua     2400

aagaaacugc ccaucgaaac cuacaacgag gaguuuauua aggaaaaugu gaagccuauc     2460

uucguauccc gggugccauu ucgcaaaauu ucagggaaac ucuucaagga gacaaucuau     2520

agugaaagag cauuuaagga uggauacuuc guuucuaaaa agagccuggu cgaccuuaag     2580

aggggugauu uggacaacuu uuacaauuau gagugugaua aaaagcugua cgacgccaua     2640

uccgaacgga ugaaagaguu caacuauaau ggcaagaagg cuuuugaguc acccuuccga     2700

aagccgacua agagcgggaa agaaggaccu guggugagau cugucaagau uaaauccgcc     2760

gcacccuuua aggauggcau cccaaugaac gaggguuuag uugcgaaaga agggauggug     2820

cgcaucgaca uuuacgagaa ggaaggcaag uauuucgugg uaccugucua cagguaucag     2880

cucgcuaaag gaaucgugcc caagcgugcc gcaauugccg gcaaaaguga gcugaagugg     2940

ccaauaaugg aucagaccua caauuucaaa uuuagcaucu ucaagaacga ccuuauugag     3000

aucaaauacg cuaagaagga uggguauuuu ggauacuaug acgguuucga ucggcauaca     3060

gccucucugg uuauugaaaa ucacgacaac uccgacagau acaggggcau cggggugaaa     3120

acuggagucg uggaguuuaa caaguaugaa guugaugugu ugggcaauua ccauaaagua     3180

aaccucggug ggaauuga                                                   3198


<210>  2672
<211>  3201
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2672
augagcgaga gaguggucag gcggauccug ggcauugacc ucgggauagc cuccugcgga       60

ugggguguug uggauaucuc uggcgcuggg ggaggcauua ucgcaaccgg uguacgcugu      120

uucgacgccc ccuugauuga uaagacaggg gaaccuaaau cagcgacucg acguacggcu      180

agaggacaga ggcggaucgu gcgcagaagg cggcaacgca ugaacggcgu ccgaagacug      240

uugugcgagu uuggcuuacu gccagacccg aggcccgaug cccuaaauca ggcaaugcgg      300

cguauaagua ccgccagcgc ugcagcccag gugacaccuu ggacucuccg cgcugccgcg      360

caccagagac ugcuuuccaa cgaggaacug gcaguugugu uggggcauau cgccaggcac      420

cggggauucc gaucuaaugc uaagaacgaa gccggugcaa augcugccga cgagaccucg      480

aaaaugaaga aagcgaugga ggccacacgc gaaggccucg caaaguacca ugcuuuuggg      540

gauaugauug ccaacgaccc aaaguucgcu aauagaaaaa ggaaccggga uaaggacuau      600

ucacacaccg ccaaacgcag cgaucuggag gacgaaguca gagcaauccu gagggcccaa      660

cuccguuuug gaagugcugc ggcaacugag acauuagccc agaccuucgc ugauguggcc      720

uuuuuccagc ggccccugca ggacuccgag gaccgaguag gcgauugucc uuuugaacca      780

ggucaaaaga gagcagccag gcgcgcuccc ucuuucgagc uuuuucgguu cuugagcaga      840

cuggccaauc uaaaacucgc aguugggagg uccccugaac gccgacugac ggcggaggaa      900

auugcucuug ccgcaaaggg auuuggcgag acuaagaaaa ccaucacauu caagucacug      960

cgggaggccu uggaccucga uccaaacgcu cguuucagug gcauagggaa agacaaggaa     1020

gccagccugg auguggucgc aagaacugga ggugcugccu acggcaccaa aacauuaaag     1080

gacgcgcugg gggaugcccc guggaggucu cucucccgca cccccgagac gcuugaccgg     1140

auugcugaaa uccugucauu uagagaggau auuacuagca ucaggaaugg auuggaagac     1200

cuaggccugg auagucucgu gguggaggca cugaugcagg ccgcugccaa cggugacuuc     1260

aaagaguuua cacgagcagg gcauaucucu gcgcgcgccg cucggaauau uauaccuggc     1320

cuuagagaag gacuggucua uuccgaggcc ugcgcaaggg uuggcuacga ccacgcugcc     1380

cguuugagcg ugccacucga ucagguaggg ucgcccguga cccgcaaggc acugucugaa     1440

gccuuaaagc agguccgggc uguggcgaga gaguauggac cuaucgacua cuuccacauu     1500

gaacuggcca gguccguugg uaaaucagca gaggagcgaa agcaacuaac ugauggcauc     1560

gaagcucgga acguggagaa agccaagcgc agaaaacagg ccgaggaaca ucucgggagg     1620

gcuccaagug acgaugagcu gcuucgguau gaauuggcaa aggagcagaa uuuuaagugu     1680

auauacagcg gagacgccau ugaucccgcg ggcgucgcug ccaacgacac acgauaccaa     1740

guggaucaca uccugccgug guccagauuc ggugacgauu cuuaucucaa caaaacccug     1800

ugcacggcac gcagcaauca gaacaagagg gggcguaccc cuuuugaaug guucgaggcc     1860

gacaaaacag auguagaaug gauggaguac guugcucggg uggagaaucu ucaggaaguc     1920

aagggcagga aaaagaggaa cuauucaauc aaggacgcag ccgcgauuga ggacaaauuu     1980

aaggcucgca aucugacuga uacccagugg gccacacgau ugcucgcaga cgaacugagu     2040

agaauguuuc ccccacggga gugugaaagg gccaucugcg gacgcgcuga uggcgggaac     2100

gacggauuaa cuauugugga ggagcguaga gucuucaccc ggccuggugc cauaacaucc     2160

aaacugaggc gcgcaugggg ccuagaaggg cucaagaaac aagauggaaa gcgaguggag     2220

gacgauagac aucacgcugu ugacgcccug gugcuugcgg ccaccacuga aagccuucug     2280

cagcggcuca cgguagaggu ccagaggcgc gaaagagagg gcaggcccga ugacaucuuc     2340

cacugugcag agccauggcg ggguuuucgu gcugaugugc gccgaacagu uuacgggucu     2400

gaaaccaugc ccggcauuuu cgugucaaga gccgagaggc ggcgcgcuag aggaaaggca     2460

caugacgcca cuaucaaaca gaucagggaa auugagggcg agcggcuggu cuucgaacga     2520

aagccugugg agaaauuaac cgauaaggac cuggagaaaa uaccaauccc gaagcccuau     2580

gggcaaguaa gugacccuaa aagacuucgc gaugaauugg uggaaagccu cagggccugg     2640

auugcugcgg gaaagccaaa ggaccggccc ccucuguccc caaaaggcga uguuauccgu     2700

aaggucagga uucagacaaa ugacaaagug ucuguggaga ucaacggugg gaccgucgau     2760

agaggagaca uggcacgcgu ugauguguuu cggaagaaaa auaagaaggc cgugugggag     2820

uacuauguag uccccauaua cccgcaccag aucguggcuc uaaacgaccc uccagaucga     2880

gccguuauug cauacgccga agacaaagau uggaaggaga ucgacucgag cuaugaguuc     2940

cugugguccc ucuuuggccu gucauacgug gaaauuagua aagcuaaugg ugaaugcauc     3000

gacggcuauu ucagagggcu ucauagggga acuggcgcgg ccucugucug uaagcacauu     3060

agcuugggga aagaugcaac aguguccgga auaggccuga agacguuagc uucauuuaag     3120

aaauucacca ucgaccgccu cggucggaag uuugagauuc ccagagaggu ucguacuugg     3180

agaggggaag ccugcacaug a                                               3201


<210>  2673
<211>  3201
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2673
augagccacc uggugcucgg ccuugaccug gggcccaacu ccaucggaug ggccuugguc       60

aaugaugacc cugagcaucc aguugaaucu aaguuagugg aucugggugu aagaguguuc      120

ccggagggcg ucgacaacuu ugauaccuca aaagaacuaa guaggaauga ggaccggcgc      180

guggcucgag ggaugcguag acagauuagg cggcgcgcaa gaaggcggca acagcucaag      240

caggcccuga uagauagcaa acuuuggccc uccgacccua acgaugaagu ugugcuguac      300

aauacaaacc cauaugaguu gcgcgcgcga gcucucaagg agagacugga accccacgag      360

gucggaaggg ugcugcucca uuuaaaucag cggcguggcu uccugucuaa ccgcaaaaag      420

gacagaggug auaaggaagu acaagggaug cuugccgaga ucaaugagaa cgaacagcag      480

ugcaaagacg gaggcuuuga uacuauuggc gcaugguugg ccgacaagca ggcuaauauc      540

gaccacgcaa acagggagga aaaugaucau guucggaacc gacaccuggc ccgcucgcaa      600

uacguggacg aguucgaagc uauuugguca guccaggcca aacaccaucc uaagcuacuc      660

acggaggagc ugaaaguggg gaaacuugga aagcagaccu auccagugaa gcccaucccu      720

agauggcacg aacauaggca gggucugagc ccauuggaga guuuuggcau auacgggcuc      780

aucuucuuuc aacggccgau guauuggccc aaguccgucg uuggacugug ugaauuagag      840

ccuaaagaac gcagaugcca gaggucugau cgucggcagc aacgauucag acugcuccag      900

gaggugaaua accuuaagua cguagaccca gauacacacc uggagcagaa auuggacgau      960

aagcagaggc gccuacugcu cgacaaacug agccacacua aggaaaugac cuuugaucaa     1020

auucggaagg cgcuugacuu ccuggagucc gugaaauuua acuuggaaag aggcgagagg     1080

ucacgccuca aggguguccc caucgaugca cuguucgccg cuaaagacgu guuagggcag     1140

gaaugguaug accgaccuga gcagcagaag acagagauug uugccgugcu gcuagauaau     1200

gaacgggacg augaccguau cauagcaaga gcugucgccg aguggggaau gaccgcgaaa     1260

gaagccgagg caaugcucgg cguggaucug ccagcuggcu acgccaaccu uaguuguaag     1320

gcuauugaga aguugcugcc ccauauggaa agggggcucc uguacaugga cgaugacgag     1380

agcaauucug ccaugcacgc agccggauau cuucgccggg aucaacugag aaggcgaauc     1440

uuugacguau ugccggaucc ugcucgcacu cccaacugcc caauugguga caucccuaau     1500

cccguuguga aacggacacu caccgaaauc agaaaggucg ugaacgcgau ugucagggag     1560

uacggcaaac cagaugcaau acaucuggaa uuagcccguu ccgugcagau ggggaaggag     1620

aaacgcucag aguauagcaa gaugauccgg gacagagaag cugagaggga cggaauuguu     1680

gaaaagcugc gagagagugg cgugcgggua acgcgcgaua auauccuaaa auaccagcuc     1740

ugggaacagc aaaaccagga guguauauau ucugguaagc ccauuuccuu cgagaaacug     1800

uucggggaag gcggaggcgu cgagguggac cacauccuuc cuagaagcag gucguuggau     1860

gacucucagg ccaauaagau cguuugccug cggacugcaa acgccgggaa aggagaucag     1920

accccauacg aauggcucgc ugacacacaa ccgaagcagu augaacaggu gugucaacga     1980

gccgguaugc ugaugagauc cggccgcaug cccuacgcaa aguacaggcg uuuuauucag     2040

cgggaguuag agcuggauaa auucaucgcg agacagcuug cugacacugg guauauuacc     2100

aaggccacaa uggaguacuu gagguaucuc uuugauaaag accaugaugu ccugggacua     2160

aagggccagc ugaccgcaga acuccgcuac cacugggguc uggagacgau acuugaagag     2220

uugccugacu caccagccug gcacgaggcu gauaguggga aacugcgacc cggcgaaaag     2280

aauagagccg accaucggca ccaugcaauc gacgcugugg uaauugccuu aacuaacagg     2340

cgccgucucg ugcaacugag cgauaucguu aaaagaggag gcgcgcgggc ccacggggag     2400

auccuugaag acccuuggcc aaauuucagg gagaacgucg ugcaggcuau uaagaagaca     2460

aauguguccc accgcgucga acgaaaaguu gcaggaaagc ugcaugagga gaccuuguau     2520

ggucccacua gaacaccggg cgaaugggug cuccggaaac cugugauagc ccugucugcu     2580

aacgagguag aaaggauccg cgaugccacc auuagacaga ucgucgugag cgcacuagcg     2640

gacgccggga uugauguugg aaggggcaag aaaccagacg cuaagaagau gaaagaggcc     2700

cugucaaagc ucaaaaugcc caguggugug ccuauccgga agguccgucu gacgaaaccc     2760

gaacagacca uacaaccagu gcgcgcaggg cgaccugauc aggcuuuugu uaagcccggc     2820

uccacacacc aucuuugcau cuucgaguuu acugagaacg gaaagaccaa aagagacgcc     2880

guguucguaa caauguugga agcaaugaau aggcugaagc ggcgcgagcc agucauucag     2940

agaacucacc cggaaaggcc ugauagccgg uuugugaugu cuuuaucagc ccgagagaug     3000

cuccuggcua acuggaaaga cggcgaaaga cuucugaccu ucaagacagc gaguagcacc     3060

caggggcaaa ucugguuugc cgagcauacu gaugcacgca aauccgcuga cuacaagcag     3120

uucguggcca augccaacac gcuagacgcu aagaaaguca caguugaucc ccucggaagg     3180

auucgguggg caaaugacug a                                               3201


<210>  2674
<211>  3204
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2674
augggcacua gacuggggcu cgacaucgga acaaacagca uugguuggug ccuuuucaau       60

cuggauggcu ccgggaggcc cauaggaauc cgggacauug gcgugcgcau cuuuucugau      120

ggucgagacc cucguucagg ggccaguuug gcugucgaga gaaggguugc acgggccaug      180

cgcagaaggc gggaucgcua cauucgaaga aggagcgcgu uacuggaagc ucuaaucaac      240

acuggacuca ugccagccga cgugcaggag uccaaggcac ugacgggcug ugauccguau      300

guacuucggg cccaagcucu ggaccgugca uuggaugccc accuccuggg ccgcgugcug      360

uuccaucuca aucagagaag gggguuuaaa ucuaaccgga aggcugaacg aaaagccgga      420

gacgauaagg aggguggcaa aauagcggac ggggcaaagg ccuuagaucu ggcuaugcac      480

gaagccggag cacgcaccua cggcgaguuc cuugcuggua gagacucgag gcggguccgc      540

uugagagccg agggggacgg auaugauuuu uaccccgaaa ggcgucaucu ggaggcggaa      600

uuugacgcca ucugggaugc acaggcugcc uauaauccug cucuacucac agacgugguu      660

cgggaucgac ugagaaggau uaucuuuuuc cagcgcccac uuaaggcccc caaagugggc      720

ggcugcacuu ucuucaacga ggagccucgg cugccaaagg cacaccccuu guuucaagaa      780

agaaggcucu acgaggaagu caaucagcug gagauuaccc gcgccggggc uccuucacga      840

aaauuaacac uggaccagcg ggauaagauc auacguaaac ucaagagcgc gaagaccgca      900

aguuucuccg gacuugccag acuguugaaa cuagcugacg gugaaucuuu uagcagggug      960

acugagaacc gcacaggccu ggccggggau gacauuuuca ccuccuucuc agauaagcgg     1020

uguuuuggag acagaugggc acauuucgac ccaggcguac aguggaguau cauugagacg     1080

guuacugaag aggaagaucc ggugaggcuc cgagacuggc ugauggccac ccacgguguc     1140

acaguggagc aagcugaagc caucgggcgc gugcggcuuc ccgagggcca cggaagacug     1200

ggcgagacug caagcagguu gauccucgcg cagcugaaau cuggguccaa ggauggacau     1260

ccuuuaaccu auaaugaagc ugucgaggcc gcacugggua aaucacacag cgacuuucgu     1320

acaggcgaaa uucuagagca gcucccauac uacggggaaa uacugagucg cgagauuccc     1380

ccuggaucuc uugauccaga cgaugacgau gagauccggu ggggcaagau caccaacccc     1440

acgguucaua uagguuugaa ucagcugaga aggcuccuga acgcccuuau uaaagcucac     1500

gggcgaccgg acgaaaucgu gguagagcug gcccgggaau ugaagcucuc cgagaaggau     1560

aaagcagagc acaaucgcag aaucggcgcu aacacuaggg ccgcggaagc ccggagcaag     1620

aaacuguuag agucgggaca accugacaca ggcgcuaauc gaucucugcu aaagcucugg     1680

gaagaucuga accccaacaa uccacuugac agaugcuguc cuuucugcgg gcgccauauu     1740

ggacagaugg cauuguuuuc cggugagacc gaaaucgagc acauuauacc cuauucaagg     1800

agucuggacg auagcgccgc uaacaaagug aucgcccauc guuccuguaa ucgggagaag     1860

ggcaacagaa cuccauggga agcauggggg cacacagaga ggugggcgau uaucgccgaa     1920

caggucucuc gccuccauaa gagcaaacaa uggcgauucg cucccgacgc caucgagaga     1980

uuuaaucagg aaggaggcuu cauugcacgg cagcugucag auacccagua cuuaaguagg     2040

auggcucgca cguaucuguc cagccuuuac ccugacaccg ccggugugua uguuauacca     2100

gggcguauga cagcaauguu gagacggcuc uggggccuga acgagcuacu gccggaucac     2160

aauuacgugg agaaccccca cucuggagcc ccuaagaaua ggcucgacca ucgccaccau     2220

gcuaucgaug cggccgucgu gggcguaacu acccgagggc ugcuuaacga aauuucaaga     2280

gcagcuggac gggccgagag gcaagacuug gaucgccugu uuaucgacuu accaaaaccc     2340

ugggaaacau ucagagagga ucuccacgaa agucuggcca ggguuguggg cagccacaag     2400

gcugaccaug gucggaaagg ggucccugca aagggaaagg augccacugc ggcucgucuu     2460

cacaaugaga ccgccuaugg ccugacaggu uugaccuccg agucuggccu cccaauugug     2520

guccaucgcg ugccccugcu aucgcugaaa ccggacgacc ucagcaaccc uauccgaaua     2580

ccagaugcag cccugcagag agcucuuagg gaagcaacug acgggaaguc cggaaaagau     2640

uuucaggagg ccuuggcgca guucucaaag agugaccccg uuuuuaaagg cauccggcac     2700

gugcgcguaa gagaaacgcu gaaggucauu ccuaucaggg augcuucugg gcaugccuuc     2760

aaggcauaca aaggagacgc caaugcucgg uacgaugugu ggcgaaugcc cgacggcaag     2820

uggguuagcu ccuggagaga ucgcgacggu aaagcccacu cagggauuau cacaauguuc     2880

gcagcucacc aagccggaca gccagugcag aggccucauc ccgcggccaa gcgggucuua     2940

agccuccguc agaacgaucu gguggcagua gagccacaag gccagccgag aaaaauaaug     3000

agggugguua aguuuucuuc cgguggccag auuaccuucg cugagcacca ggaagccggg     3060

ccucuuaaag cucgcgacgc aaccaguccc gacaucgauc cauuuaagua uuucuacagc     3120

ucugccggag gccugcaaaa ggcccgggcu cgacagauua gaaucgacga gcuagggagg     3180

auauuugauc ccggaccucg cuga                                            3204


<210>  2675
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2675
augaaguacg ugcugggccu cgacaucggg auugccagcu gcggaugggc ugucauaaac       60

caggagaaac acagaaucga agaucuuggu guuaggauuu ucgacaaggc agagaauccc      120

aaagauggca agucccuggc caccccucgg cgcgacgcgc gaucuacacg uagaacuuug      180

aggcggaaaa agcaucgcau gcaaagaauc aagauuuuac uggugaaaca cgggcuacuc      240

ucaaagacgg aaaucgauca ucuguaugag agugcuaccg aaauagacgu augguaccuu      300

aggcugaacg ccuuggagcg gcgccucaau ccaaaagagu uugcacgagu gcugauccac      360

cugagcaaga gaaggggauu caaauccaac cggaaggaaa caacucucuc ugagaauggc      420

cagauuuuag aaaacaucuc ggagaaucug cagauuaugg agcagaagaa cuaucguacc      480

gucggugaaa ugauccuuaa agauaagcgc uuugagaauc auaaaagaaa caaggacggg      540

acauacauag gaaccgugac uaggcaacag uugcaggaag agauucagau gaucuucaau      600

gcccaacggc uguauaaaaa caaucacgcu acagaaaagu uugagucaag cuaccuagag      660

auuugggcaa gucagcgacc guaugccucc aaagaucaga ucgaaaagau gaucggcuac      720

uguacccucg agcccaagga aaaacgcguu ccuaaggcuu cuuggagcuu ccaguacuuu      780

guggcccugc aaacgauuaa caaucuuaga cugauaucca aagacaggau cgaggaauug      840

ucauucgagg agaagaaacg gauuaugaac cucgcgcugg aaaagaguau cgucaaguau      900

auagauauuc gcaaauuacu gagcgugcca aaggaguuuc aguucaacuc ucucuccuac      960

ucagcagaca ucguagauac uaccguugaa aauaaaaagu gcaucgagcu uaaagaauau     1020

cacagcauua acaaggugua caagcagauc uauggccaaa augcccugaa cuugcuaccc     1080

aaagacuacg acacaauugc uuaugggcug acuauauuua aggaugacaa agauauccag     1140

gaguaccucc agaauaagua cgucaacgcc aaaggaaagc cuauuaguaa ucuggcaaac     1200

aagaccuaug acgauacauu cauccaggug cuucugaccu ugaauuuuuc uaaaaugggu     1260

caucucuccu ucaaggcucu gaaaaacauc auuccauucu uagaggaagg ccugagcuac     1320

gacaaggccu gugagaaagc gggguauaau uuuaagggaa cgucguacac uaaaaagaca     1380

aaguaucuac ccgugauacc ucaaaacacc aauccagucg uucacagagc ccucucucag     1440

acuaggaaag ugaucaacgc aauuaucaag aaauacggcu ccccgaaugc uauucauauc     1500

gaaacagccc gugagcuguc aaagaccuuc caggagcgga aagaaauaga uaguauguau     1560

caggacaaca gcaagaagaa cgagcacgcu aucugcaaac uuaaggaauu ggguaauauu     1620

aaccccuccg ggaucaauau uguaaaauuu aagcugugga acgagcaaga uggacgaugu     1680

auguacacgg gcaaauacau cgaaccucac agacucuucg aggagggcua uaccgaagug     1740

gaccauauac ugccauacuc uaggagccuu gaugacucau auaauaacaa gguccugaca     1800

uuggggacug agaaucagcg caagggaaac aaaacccccu acgaauauau gcggaauaac     1860

aguauuuggg acgaguuuga aacaagagug caguccaaua agaaaaucaa caagaaaaag     1920

cagcaaaagc uccuguuaca gaauuucagc cacgccaggg agcaggaguu uauugaacgc     1980

aaccugaaug auacucgaua cgcaaccauc uaucuaucuu cacucauaca gcaacaucug     2040

aucuucagug agagcucccg ggagaaaaag guucacacag ugucugguau uaucaccucg     2100

caucuucgua gcagaugggg cuuuaacaaa gacaggaagg aagggcacau ucaccaugcc     2160

uuggaugcug ucaucguggc gguaacuucc gaccacauga uacagcgcgu uacgaaauac     2220

uacaagcuga aagaacucaa ccggaauaga caggcaaaga ggaugcaauu cccugagcca     2280

ugggagggau uucgacugga auuagaggcc cgcauuucac ccaacacaca gcaguaucug     2340

aagggcaucc gguucaaaaa uuacgcugau gugaaccuua gugaaaucaa gccgauuuuu     2400

gucucuagaa ugccuaaaag gagcaucacc ggugaguugc aucaggagac uauucguaag     2460

uuccucgggu auaaugaaaa aggcaaggug cugaccgcca caaagaccaa acuagaggac     2520

auacccuucg augcaaacgg agacuuucca auguucggca aggaaacuga ucuguacaca     2580

uauaaugcca ucaaagagcg cuaccucucc cacaagaaag acaagucaaa gagcuuucaa     2640

gaaccucugu auaaacccac gaagucuggg ggaauugguc cacuuaucaa auccauuaag     2700

aucauggaua cccggacuau agucaacccc gugaaucagg gcaaaggggu uguguacaac     2760

aguaagaucg cuagaacaga cguauucaag aaaggagaga aguacuauuu gauuccuauc     2820

uacaccauug aucugaugaa aaauacauua ccacagaagg ccaucacugc aggcaaaggu     2880

uaugaggacu ggaccacgau agacccgagc uuuaccuucc ucuuuucucu guuccccaac     2940

gaucuuauuc agaucguccc uuccaagaau aagacaauca aagcgaggac uaccguguca     3000

aagaaagaag uucuguugcc aagucucaca ggguacuuua agggcgugca cagcggaacu     3060

gcuggcauua ccgucgagac gcaugacggg ucgauaaucg ccaacguggg auccaaacaa     3120

cugcuaucuu ucgaaaagua ucagguagau gugcuggguc acuacacaaa aauuaagaag     3180

gagaaacgaa ucggcauggc aauuuga                                         3207


<210>  2676
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2676
augaacaagg ccguggcuua cagacuggca cucgaccuug gcagcaccuc ccuggggugg       60

gccaucuuca gguugaauga ggcgcgggaa cccacagcua uuauacgcgc cggaguccga      120

aucuuuucug auggucguaa cgcaaauuca gagccuuuag ccguucacag aaggguggcu      180

cgggcaaugc gcagaaggcg ggaccgccug cuaaaacgaa agaaaagaau gcaugaucag      240

cucguacaac acggcuucuu uccagccgaa guggcugaga ggaaggaacu ggagcggcuu      300

aacccguauc agcugcgugc caaaggguug caugaggcgc ucacucccgg agaguucgca      360

cgcgcccugu uucacauuaa ucagagaagg ggcuucaaga guaaccggaa gacggaccga      420

aaagauaaug acagcggugc ucugaagcag gccaucuccg aacuccgcaa aaggauucaa      480

gauucugacu gcgcaaccgc uggggaaugg uuuuggaagg agagaaugca gcagaaaccu      540

gagggagucc ggggccagug ggugcgcgcc agauacagga agacaccauc gacuaccgau      600

gaaggcaaga aacguaucgg guaugacuua uacguugauc gggcgauggu ggagcaagag      660

uucgacgccc ugugggcagc ucaggccgcc cuucagcccg gauuguuuac agaagcugca      720

cgagccgaac ugaaggauac ccuacucuau cagagagacc ugaggccugu caaaccaggu      780

cgcuguacuc uucugcccgc ugaggagcgg gcgccuuugg cacucccauc aacacaaaga      840

uucaggauac ugcaggaagu gaacaauuua cgccugcucg acgaggcccu ucgagaagua      900

ccgcugaacu uggcucagcg ggaugccguu gugagcgcac uagagggcaa gaaagaacug      960

aguuuugccg cuauccguaa gcuccugacc cuuuccggga aauucaaucu ggaggacgag     1020

aagagagccg aauugaaggg aaacgcaacg ucugucauuc ucgcgaggaa agaucuguuu     1080

ggcgacgcuu gggccgguuu cgaugcagcc gugcaagacg agaucgugug gcgcuuaguc     1140

agccaggaau ccgagggggc ucugauugcc uggcuacagc agcauacugg aguugauggc     1200

gugugcgcag aagcuaucgu aaauacccgg cuccccgacg gcuacgggag acugucaagg     1260

aaggcccuug agcgaauagu gccugcguug caacgcgagg ucuguacaua ugauaaagcc     1320

gugcaggcug caggauucgc ccaccacagu gaccuggguu uugauuucga cuacgcugaa     1380

gacgagguuc aacagguggg cgaacauacu auugccagca ccggggaggu ccaggcacag     1440

uacgcguuua agcagcuccc auauuacgga aaagcccugc agcggcacgu ggcuuucggc     1500

ucuggugaug ccaaggacca ugaggaaaag ugcuauggga aaaucgcaaa ccccacagua     1560

cacauuggcc uuaaucaagu uagaaccgug gucaacgcuc ugaucaggcg uuacggacac     1620

ccuacggagg uggucgugga auuggcccgc gaucucaagc agucccggga gcagaaacag     1680

caaacucaga gagaacaggc agacaaccaa aagaggaaug aggauauccg aaaacggauu     1740

gccccaauac uggagacauc acccgaacgc guuagagaca gggauaucaa gaaguggauu     1800

uuaugggagg aacugaacaa aaaggacauc gcugaucggc auuguccgua uagcggcgag     1860

cgaauaagug cgaccaugcu acucucugaa gccguggaga uugagcacau ccugccuuuu     1920

uccagaacuc uugacgauag cuugaauaac cgcacaguag caaugaggcg ugcuaaucgg     1980

aucaaagggg acagaacccc cugggaagcc agggccgauu ucgaggcuca gggauggcgc     2040

uacgaagcaa uucugcagcg agccgagggu augccaccua gaaagcggua uagguuugcg     2100

gaagacggcu accagcgcug gcucgggaaa gaccguaacu uccuggcuag agccuuaaau     2160

gauacgucgu accugucucg gcuugcagcc aacuauuuga ggcucgucug cccccaagga     2220

gugcgcguua ucccaggcca gaugaccgac aagcugcgag guaaguuugg gcuaaauucc     2280

gugcugggcc ucgauggcaa aaagaacaga aaugaccauc ggcaccaugc ugucgaugca     2340

ugugugauug gaguaacaga ccaggggcug augcagaggu ucgccaacgc gucaaaacaa     2400

gcucgcgaga auggacuuac uagauuggug caggauaugc uguuacccug guggccuagu     2460

uacuaugacc acguugagag ggccguccgg cacauacgug ugagccaucg cccagaucac     2520

gguuuugaag gcgcaaugau gaaggggacc gcccauggaa uccgagagga cggcauuaga     2580

gaagauggua ggaucaaaca gcggccgaag gcuaaagggu ccgccgacca caagacaauc     2640

acucucauuc ccauagauga gccucgccag cuggcaagac acggcgugga cgcugaggga     2700

aagccacuuc ccuacaaagg cuaugccucu gggagcaacu acugcaucga aauuaccaag     2760

aauggaaaag gcaaguggga gggucaaguc aucucaacau ucgacgcgua uaggauuaaa     2820

gccgcagcug augccgcugc acgggccggg cagguuauca guaccgugga agccgacucc     2880

auagugcgaa agagcggaug ggagagacug cgcggcgcuc agucucaaaa cggucagccu     2940

uugguaauga ggcucgucau cggcgauuca gugcggaugg aaguugacgg gcgugaugag     3000

gugaugagag ucgugaagau gaguggaaaa gagaugguuu uugcgccagu gagggaagca     3060

aacguagaca agcgcaauaa caugcccgau gagcaggacc cguucacuua cacguacaaa     3120

cgggccgauc agcugcgaaa ggcuaaagcc agacaaguca caauuagccc uaucggcgaa     3180

cuaagggacc caggguucaa gggauga                                         3207


<210>  2677
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2677
augcaguacg ugcugggccu cgacaucggg auugccagcu gcggaugggc ugucauaaac       60

caagagaagg aaagaaucga ggaucuuggu guuaggauuu ucgacaaagc agaaaauccc      120

aaggauggca aaucccuggc cgcgccucgg cgcgacgcuc gaucuacccg uagaacauug      180

aggcggaaga aacaccgcau guuaagaauc aagauucugc uagugaagca ggggcuccug      240

ucaaagaacg agcuuaauca ucuguaugaa aguacuacgg agguagaugu guggaacuug      300

aggcucgacg cccuggagcg gaagcugaau ccaaaagaau uuacccgcgu ccucauccac      360

uuagcaaagc gaagaggauu caaaagcaac aggaaggaga caacucuguc cgaaaauggc      420

cagauacuug agucuaucuc ggagaaccag caaauuaugg aacagcagaa uuaccggacc      480

gugggugaga ugaucuugaa ggauaaaaag uuugaaaacc auaaacguaa uaaggacggg      540

acauauauug gaaccguuac ucgccagcaa cugcaggagg aaauccagau gauauucaca      600

gcccagagac uauacaaaaa cgauuaugcu accgaggagu uugaaauuuc auaccucgag      660

aucugggcaa gccaaaggcc guaugccagu aaggaccaga uugagaaaau gaucggcuac      720

uguacgcugg aacccaagga aaagcgggug ccuaaagcau ccuggucuuu ccaguacuuu      780

gucgcccuuc aaacuaucaa uaaccugcga uugauuagca aggaucgcau agaggagcuc      840

uccuucaaag aaaagaaaca gaucaugaau cuggcguuag agaagucaau ugugaaguau      900

aucgacauaa gaaaacugcu caguauucca aacgaauuuc aguucaacag ccuuucuuac      960

uccgcagaua ccguagacac aacucuggag aauaagaggu gcaucgaauu gaaagaguau     1020

uacucaauca acaagguuua uaaacagauu uacgagaaga augcccuaaa ccugcucccc     1080

aucgacuaug auaccauugc uuacggccug acaauauuua aggacgauaa agacauccuu     1140

gaauaccugc aaaauaagua ugugaacgcc aaagggaagc cuauuaauaa cuuggcuaaa     1200

aagaccuacg auuauacguu cauccaggcu cuccugacuu uaaauuuuac aaagauggga     1260

caccugagcu ucaaagcccu aaagaacauc auuccauucc ucgaggaagg ucugaguuac     1320

gacaaagcgu gugagaaggc cggcuauaau uuuaaaggga ccucuuacac ugagaagaca     1380

aaauaucuuc ccgucauacc ucagaacacc aauccagugg ugcaucgggc auugucccag     1440

acgcgcaagg ucaucaacgc uauuaucaag aaauacggaa gcccgaaugc cauucacauc     1500

gaaaccgcua gagagcuguc gaagacauuc caagaaagga aagagauaga uucuauguac     1560

caggacaacu ccaagaaaaa cgaacacgcc auccauaagc ucaaggagcu gggcaauauu     1620

aaccccucag guaucaauau uguuaaauuu aaacuuugga acgagcagga ugggcguugc     1680

auguauagug gaaaauacau cgaaccucac cggcuguucg aggaaggcua uacugaggug     1740

gaccauauau ugccauacag ccgaucccuc gaugacucuu auaauaacaa gacccugaca     1800

uuaggcacug aaaaucagag aaaagggaac aagacccccu acgaguauau gggaaauaca     1860

agcauuugga acgacuuuga gaccagggua cacucaaaua agaaaaucaa caagaaaaag     1920

caacagaaac ugcuacucca gcacuucagu uacgcacgcg aacaggaguu uauugaacgg     1980

aaucugaacg auacuagaua cgccacgauc uaucuuucca gcuugauaca acagcaucug     2040

aucuucucug agucaaguag gaagaagaaa gugcacacag ucagcgguau uaucaccucc     2100

caucuccgcu cucgaugggg cuuuaauaag gaccggaaag aagggcacau ucaucacgcu     2160

cuggaugcgg ugaucguugc agugacuucg gaccacauga uacagcgugu caccaaguac     2220

uauaaauuaa aggagcugaa cagaaaccuu caagccaaaa ggaugccuuu cccagagccc     2280

ugggaaggau uucgcuugga gcucgaagcu cggauuagcc cgaauacaca gcaguaccug     2340

aagggcuuca gauuuaagaa cuaugccgau gugaaucuau ccgagaucaa accuaucuuc     2400

guaucaagga ugcccaagcg aaguauuacc ggugagcugc aucaggaaac uauccgcaaa     2460

uucauugggu acaacgagaa gggcaaaguu cucacagcaa uaaagacgaa gcuggaagac     2520

aucccauuug augccaaugg agacuucccu auguauggca aagagaccga ucuuuacacu     2580

uacaacgcua uuaaggaacg guuuuugucu cacaaaaagg acaaaagaaa gagcuuccaa     2640

gagccccugu auaagccaac aaaauccggg ggaaucgguc ccuuaauuaa gucaaucaaa     2700

auaaucgaua ccaggacaau ugugaauccu gucaaccagg gcaagggggu ggucuacaau     2760

agcaaaaucg cccguacuga cguguuuaag aaggaugaga aauauuaccu cauuccaauc     2820

uauaccauag accugaugaa gaacauucuu ccgcagaaag caaucacggc gggaaagggc     2880

uacgaagacu ggaccacaau cgaucccucu uucacuuuuc uguucuccuu guuuccuaau     2940

gaccucauuc agauaguucc aaguaaaaac aagaccauca aggcucgcac aacugugagc     3000

aaaaaggagg uacugcuacc cucucugacc gguuauuuca aaggggucca uuccggcacg     3060

gccggaauua caguggaaac ccacgauggc ucaguuaucg caaauguggg gaguaagcaa     3120

cuccugcuua uugagaaaua ccaggucgac aucuugggac acuacacuaa gauaaaagaa     3180

gagaagcgga ucgguauggc cauuuga                                         3207


<210>  2678
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2678
augcccaaga gcgccaaacc ucagcaggcu uacacccugg gccucgacau cgggauugca       60

uccgugggag ccgcgcuucu gggucaacag cacauaaucg gcuugcaugu cagaacauuc      120

gauagggcug agacugccaa ggaaggggag ccauuaaaca aaauucggcg cgacgcacga      180

cugacgcgua gaaggcuaca gcggcgcgcc cacagacucc ugaggcuucg gcgccuguuu      240

aagcgacaua auuugauccc ggauaccgac acaucuacuu ucauuucagg acaaaguccc      300

ugggaacuca gagcucaggg ccuggauagg cagcugacca gcgcagagug gacaaccguu      360

cucuaucaca ucgugaaaca ucgggguuuu cacuccaacc guaagucuga aguaaagacu      420

gacgagaaag ccgggcagau guuaucggga gugaaucaaa accaggcucu gcuuaagcag      480

ucaggcuugc gcacaauggg cgagcuggcc gcgagacacg aagcauucag ggaggccaaa      540

cggaauaaga aaggggcuua caaccauacc uuuagccgac acgaucuaga agacgagcuc      600

caucugcuuu ucagucgcca gagagagcug ggaaauccuu augccacgcc agaauuggag      660

gaugcagucc acacucuccu gaugcaaagg cggcccgcuu uauccgguac caaccugcuc      720

aagauggugg gccacugcac acuugaaccu gacgaguuuc gcgccccaaa ggcgucuuac      780

acugccgaaa gauucauaug gcugaccaaa uugaauaacc uaaggguuag cggguccgga      840

gagacacguc cccugaauga gucagaacgg caggcacucc ugaguaagcc uuuuagccag      900

ucuaaacuua ccuauaagca agugcgaaaa auccuggagu ugccagauaa cauuagauuc      960

gucggccucc auuaccaccc ggcucaggac aaugguaagu cccccgaaaa caggguguuu     1020

uucgaggcca aggcuuuuca ugcccugcgc aauguauaua aaaaggcagg guuaaaccug     1080

gaauggcagc gggauucaca gaacccugag aaacuagaca auaucacgua cgcccucacu     1140

guuuucaagg aggacaaaga agcuaagcaa uggcugauug agaaaggagu ggaagcgagc     1200

gucaucgagg cagugcuuaa cgaaaguuuc ucugaguuug ugagauuguc cauaaaggcc     1260

cugaggaaga uuaucccaca cauggagauu ggccaucgcu acgaugaagc ugucaaacuc     1320

gccggcuaua gccaccacuc gcaucugccc cagccuucua agacaccaua caucccccga     1380

uucuccaaac agucaaucgc aaauccgguu gugagucggg cccuuaacca ggcucguaag     1440

cugguaaaug ccauugugag agaguauggg ccucccgacg caguccacau agaauuggcg     1500

agggaucuca acaaaccauu ugacgagcgc cggaagaucg agaaggauca agaaaaauac     1560

cagaaggaca aagcuagaga uauugagacc uuccaggaaa auuuuggauu cacuccuaag     1620

ggucugcagu uaacaaaaug gaggcuguau cgagagcaaa gcggcaagug ugccuacucc     1680

cuaaagcccc ucgacaucaa ccgccuguuu gaagaugggu auguggagau agaccaugca     1740

cuuccauacu cucggagcuu caaugaugga augaacaaua aaguuuugac ccugacgacc     1800

gagaacagaa auaagggcaa ccagacaccc uacgaauauc ucgacggugc cucaaauagu     1860

gcucagugga ggaacuuugu ggccuggguc aacuccaaua aaagcuaccg ugcaucuaag     1920

cagcgcaacc uguuacggaa agacuucgcu gcccaagcgg ccacugaguu uagagaaagg     1980

caccugaccg auacacgaua uauugcucgg gaguucaagc gcauggugga aacucaccuu     2040

aaauugaaua agccuaacca gagaugcgua guugugucag ggcagcucac cgcauaccug     2100

agggcccggu ggggccuaau caagguccga gagaauggag accugcauca cgcucucgau     2160

gccgcagugg ucgcggccug uagucaugcu auggugaaaa gacuggccga cuauagccgc     2220

aggggcgagc uucaauacgc acgugggcac cauuuugauc cagaaacagg agagaucauu     2280

gacaucaacg cuuugcggaa gcuggaugac cacuucccgc agcccuggac cgauuucaga     2340

agggaauuac ucgccuggcu guccccugac ccagcacagc agcuuaaaaa ucuggccuau     2400

uacacugaug agcgcuugaa gcaaguuaaa cccauucgag ugucuagagc uccuacgcgg     2460

aggggucucg gcgcggccca ccaggaaaca auacgcucgg caaaguaccu gggggagaac     2520

cguagcaccg uaaagacucc acuagagaaa cugaagcuca aagacauccc caauauuguc     2580

ggauaugaag auccgagaaa ccaucagcug auccaggcua ucucccaacg gcuuaccgag     2640

cacggcgacg acgguaagaa agccuuuaag gaaccuuugu acaagccagg gaauaacaaa     2700

ucaacagccc ccgugguuag gagugugaag cuguucaccg uccagaaauc uggcauuccu     2760

gugcgcggag gcauagcuaa uaacgggaau augguacgag uggauaucuu uacuaagaaa     2820

gagagauauu ucgcaguucc cgucuacgug agcgacgccg cgcggaacga guuaccaaac     2880

aaggcugugg ccaggggaaa ggaugguugg auugacaucg augacuccuu uucauuccuc     2940

uuuagccugu auccuaauga uugggucucu guugugguga aaccccauga agcaccaugc     3000

gagggcuacu augccgggcu ugacauuucc acaggagcua uccacauaug ggcacaugau     3060

cgcaacagaa guguaggcaa gaauggucug augaggagca ucgggauuaa aacggcccac     3120

ucugucgaaa aguaccacgu ggaccuacuc ggcaaccugu accggguugu gcaugagacc     3180

cguaaaccgu ugcgcgcggg aaaguga                                         3207


<210>  2679
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2679
augcaguacg ugcugggccu cgacaucggg auugccagcu gcggaugggc ugucauaaac       60

caagagaagg aaagaaucga ggaucuuggu guuaggauuu ucgacaaagc agaaaauccc      120

aaggauggca aauccauggc caccccucgg cgcgacgcgc gaucuacacg uagaacucug      180

aggcggaaga aacaccgcau gcagagaauc aagauuaucu uggugaagca gggguuacug      240

ucaaaaacgg agcuagauca ucucuaugaa agugcuaccg agguagacgu guggaaccug      300

aggcuugaug cccuggagcg gaaguugaau ccaaaagaau uuacacgcgu ccucauacac      360

cuggcaaagc gaagaggauu caaaagcaac aggaaggaga cuacccuguc cgaaaauggc      420

cagauccucg agucuauuuc ggagaaccaa cagaucaugg gucagaagaa uuaccggaca      480

gugggggaaa ugauuuuaca ggacaaaaag uuugagucac auaaacguaa caaggaugga      540

accuauaucg gcacuguuac acgccaacag cugcaggacg aaauacagau gauuuucaau      600

gcccaaagac uuuacaaaaa caauuaugcu accgcagagu uugaaaacag cuacuuggag      660

aucugggcca gucagaggcc guaugcuucc aaggaucaga uugagaaaau gaucggcuac      720

uguacgcugg aacaaaagga gaagcgggcc cccaaagcgu cuuggagcuu ccaguacuuu      780

guggcacuac agacuaucaa uaaccuccga cugauuucca aggaccgcau agaagaacuu      840

ucauucgaag agaaaaagca gaucaugaau cuggccuugg agaaaaguau ugucaaguau      900

aucgauauaa gaaagcuccu gagcgugccu aacgaauuuc aauucaacuc uuuauccuac      960

ucagcugaca ccguagacau uacaguugag agcaggaaau gcaucgaacu gaaggaguau     1020

cacaguauca auaaagugua caagcagauu uaugagaaaa acgcccucaa ucuucugcca     1080

aaggauuacg acacuaucgc auauggguug accauuuuua aggaugacaa agauauacua     1140

gaguaccugc agaacaagua cgucaaugcu aaaggaaagc ccaucucuaa cuucgccaau     1200

aaaacauaug acgauaccuu uauucaggcg cuccugaacc uuaauuucac gaagaugggu     1260

caucuguccu ucaaggccuu gaaaaacauc aucccuuuuc ucgaggaagg ccugagcuac     1320

gacaaggcau gugagaaagc uggguauaau uucaagggaa cuucguacac agagaaaacc     1380

aaguauuuac cagugauucc ccaaaacacu aauccugugg uccaccgggc ccugucucag     1440

acacgcaaag uuauaaacgc uaucauuaag aaguacggcu ccccaaaugc cauccacauu     1500

gaaaccgcaa gagagcuauc aaaaacguuu caggaaagga aggagaucga uaguauguau     1560

caggacaaca gcaaaaagaa cgaacaugcc auaugcaaac ucaaggagcu ggguaauauc     1620

aacccguccg ggauuaauau cgugaaguuc aaacuuugga acgaacaaga uggacguugu     1680

auguacucug gcaaguacau ugagccccac cgguuguuug aggaaggcua uaccgaggua     1740

gaccauaucc ugccuuacag ccgaucacuc gacgauaguu auaauaacaa aacacugacu     1800

cuugggaccg aaaaucagag aaagggaaac aaaacaccau acgaguauau ggguaauacu     1860

uccauaugga acgacuucga aaccagggug cagagcaaua agaagauuac caaaaagaaa     1920

cagcaaaagc uguugcucca gcacuuuucu uacgcucgcg agcaggaguu caucgaacgg     1980

aaccugaaug auacaagaua ugcgacuauu uacuuaucaa gucugaucca acagcaccua     2040

auauuuagcg aguccucuag gaaaaagaag guccauacgg ugucgggcau cauuacaagc     2100

caccuccgcu cccgaugggg guucaacaaa gaccggaagg aaggacauau ccaccaugca     2160

cuggaugccg uuauuguggc ugucaccuca gaccacauga uccagcgugu gacuaaauac     2220

uauaaacuua aagaguugaa uagaaaccug caggccaaga ggaugcccuu uccugaacca     2280

ugggagggcu uccgccucga gcuggaagca cggauaaguc ccaacaccca acaguacuua     2340

aaagguuuua gauucaagaa uuaugccgau guaaaccugu cugagauuaa gccgaucuuc     2400

guuagcagga ugccuaaacg auccaucaca ggggaacuuc accaggagac cauucgcaag     2460

uuuaucggcu acaaugagaa aggaaaggug uugacugcua uuaaaacaaa gcucgaagac     2520

auacccuucg augccaacgg cgacuuucca auguauggga aggagacgga ucuguacacc     2580

uauaaugcaa ucaaagaacg guuccuauca cauaagaaag acaagagaaa gagcuuucag     2640

gagccucugu acaagcccac uaagucugga gguauuggcc cacucaucaa auccauuaag     2700

aucauagaua caaggaccau cgucaacccc gugaaucaag ggaaaggagu cguguacaac     2760

aguaagauug cgcguacaga cguuuucaaa aaggacgaaa aguauuaccu gaucccuauu     2820

uauacuaucg aucuuaugaa aaauauauug ccacagaagg cuauuaccgc cggcaaaggu     2880

uacgaggacu ggacgaccau cgauccgagc uuuacauucc uguuuucuuu auuccccaac     2940

gaccucaucc agauugugcc uuccaagaau aaaacuauaa aggcacgcac cacaguauca     3000

aagaaagagg uccugcuucc aagucugacu ggguauuuua agggcgugca cagcggaacc     3060

gccggcauca cgguugaaac acaugauggg ucggugauug cuaacgucgg auccaaacag     3120

uugcuccugu ucgagaagua ccaaguggac guacuagguc acuauaccaa aaucaaggaa     3180

gagaaacgga uuggcauggc caucuga                                         3207


<210>  2680
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2680
augaagaaac agccccaaag auacauccug ggccucgacc uugggauugc cagcguggga       60

ugggcucuga uauuggucga uggugcagag aacccuguug gcuuacugga cugcggggug      120

aggaccuucg aacgggccga gguaccaaag acaggagauu cccuagcgcu cgcucgccga      180

caggcccguu cugugagaag gcugauccgg cgcagagcac acaggcuucu gcgguugcgc      240

cgacuccuga aaagagaagg cauucuguca gccgcugacu uuaauaguga uggucucccg      300

aacuuaccca ucgacgcaug gcagcugagg gucgccgggc uugagcggaa guugacuaau      360

caggaauggg cugccgugcu gcuacaucuc guuaaacacc guggauaucu gagccaacgc      420

aaguccgaga cgcagaccga gaacaaggaa cuuggcagac uguuggcggg cguggcagaa      480

aauucuaaac uccugucgga gucaagcgag caguacagga cuccugccga guuagcuguc      540

aagcuguucg ccgaacagag ugggcggcuc cgaaacaaag gaggugauua uacacauacc      600

uuuaaucgcc uugaccugca aaaggaguug caccuacugu ucgaaagaca gugugcacuc      660

ggcaacccau uugcuuccgc cgagcugcag ucuaaagugg augaacuucu gaugacacag      720

aggagcgcgu ugcaagggga ggccguacuc aagaugcugg gaaagugcac cuucgagccc      780

uccgaauaca aagcagcuaa gaauacuuau ucagccgagc gguuuauuug guuaacaaaa      840

cugaacaauc uacgcauccu cuacaacggc gaagacagag cucuggauga gggugaaagg      900

aagcagcuuu uggacgagcc uuauaaaaag gccaaacuga ccuacaagca gguucguagu      960

auacucaauc ugccagagag cgcaguguuc aaggggcuuc gguacggaaa agauucugac     1020

ggccugaagg ccgaaaacgg cgcuuugaug gagaugaaag cguaucauaa gauccgaacg     1080

guccucgaaa aagcagggcu gaagggagag ugggaaaaua gaauuaggaa ccccgagauc     1140

uuagaccaaa uugguacugc cuuuuccgug uacaagaccg augaggacau caaagcugaa     1200

cuggccgcac uacgccuccc ugagaacgug cuggaugccc uuuuggcugg ccugaauuuc     1260

gacaaguuua uacagcucuc acugaaagcc uuaagcaaca uucugccacu uauggaacgg     1320

gggaucagau augaugaggc auguaaggag gucuacggag accacagggc guuggcugaa     1380

aguaaaaauc agaugcuccu gccgcagauu cccgccaacg agauccgcaa uccuguugug     1440

cuacgaacac ugacucaagc acggaaggua aucaacgcca uuauacguag auauggcucu     1500

ccacagaggg ugcacaucga aaccgcucgc gagcucggua aguccuucaa agaucggaag     1560

gaaauugaga aaagacagga ggaaaauagg aaggagcgag aacgcgccgc agagcauuuu     1620

aaagaacugu ucccccgguu cacaggggag ccuaagagcg gcgacauccu uaaguugaga     1680

cuguaccagc aacagcaggg aaaaugcuua uauucgggca uaccaauuga ucucgggagg     1740

cugaacgaga agggauacgu cgaaaucgac cacgcccuuc ccuuuucucg uaccugggau     1800

gacuccuuca auaacaaagu gcugguuuug gguucagaga aucagaagaa acgcaaccaa     1860

acgccguacg aauaucucga cggcgcucgg gauagucaga gauggaggga guuucaggcg     1920

cgagugcagg ggugucgguu cccuuacgcc aagaaacaac gcauccagac ugcuaagcug     1980

gacagcaaga cagaacaggg auuucuagag agaaaucuga acgauaccag guauauugca     2040

cgguuccucu gcggcuuuau cgagaaaucc cugcaucuuc aaggugccgg gaagcgaaga     2100

gucuucgcuu cuaauggcca gauuacugcc uugcugcgcg gauuaugggg ccucaaaaag     2160

gugagggaag acaacgaucg ucaccacgca cuggacgcga uaguaguugc cuguagcaca     2220

gugucaaugc agcagaaaau caccaaggcu augcaacgga gagagacgcu ugaaaccauu     2280

aacacagaga cuggggaggu caagaccagg aucccccagc caugggacuu uuuccgccag     2340

gaagugauga uccgagucuu uagugaugcc ccuagagagg aacuggcaga gaaacuaaau     2400

ucccggcccg aagcucucca ugauuucgug agcccacugu uuguuucuag ggcccccaac     2460

cgcaagauga caggacaggg ucacuuggag acugugaaau cagcaaagcg ucugagugag     2520

aauauuagcg uagucaaaaa gccuuuaacc gcccucaagc ugaaagacau agaaaacauc     2580

gugggcuacc cagauagaga gccggcucuu uaucgggcgc ugcaagaaag guugcaggcc     2640

uacaaggacg aucccgcaaa ggcuuucgcc gagccuuucu auaaaccagc caaagacggg     2700

ggacagggcu cccucguuaa ggcugugcgc auuaucgaaa auacaaagca uggucugaaa     2760

guccgaaacg ggauuaccga cacuaauggc gauaugguga gagcagacgu auaccagaag     2820

gccgauaaaa agggaaaacg gcaauacuuu aucgugcccg uuuaugcgug gcaggucgag     2880

aagaacgagc uaccuacggu ggacugcaaa ggcuacgaaa uagaugacac auaugaguuc     2940

uguuuuucuc uguacaagua ugauaucauu gaaauccaga auccagacaa caugucgaag     3000

gagcuuaggg uguacuauau uaauugcgau agcuccgacg ggcgcuucug gcucgcucac     3060

caugauaaau cagccaagga acaacaguuu agaaucagua cccagaacca gcccauaauu     3120

aaaaaguacc aagucgaccc gcugggaaaa gagaucaggu uguguaaggc agagaagcgg     3180

acugaacuga auggcaaaaa gaaaaaguga                                      3210


<210>  2681
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2681
augaacuacg acaucggccu gagaauuggg auaaccagcu gcggaugguc caucauuaau       60

aaggaucucg agaggaucga agaccuuggu gugcgggucu ucgagaaagc cgaaaacccc      120

gauggcacag cuucugcagc cccucgccga gaggcgcgua agucaagaag gaaacaccgg      180

cgcaagaaac auagaauuga aaggaucaag cggcugauag uucagcacga cuuguuaagu      240

aagaaagagc uggauagccu auaucucucc ccauuuaaua ucgacgugug gaaccugcgc      300

guagaggcuc uugaacgaaa gcuggauaau agagaguucg ccaggguguu gauucaucuc      360

guccaacggc guggguuuca gacuauccgc aaaucugugg aaauucagga ggagggaaag      420

cugcuggaaa acguuucgga gaaugaaaga aucaugaaag ugaacggcua caagacgguc      480

ggugagaugu ucucaaagca cgaaaaauuu acccaucaca agaggaauaa agacgggagc      540

uauacaagug ugguaacucg gucccucuua cugaaggaga uaaaaaccau uuucaacucu      600

cagcgaaucc uuggaaaugu uuuuagcaca caaaaguugg aguccgauua ccuguauauu      660

uggggcucac agcgcccgac ccuaacuuac gaacagcuca ugaguauggu gggcaacugu      720

aucuaugaga aaaaggaaaa gagagcaccc aaaacaagcu gggccuucca guacuuuucu      780

cugcuucaaa agaucaauaa acugaagguc uccgacgaua acaccuugag gaaacucuca      840

aaggaggaac gggacauugu gauagagcug gcuuucaaga aaaagaaugu gagcuuuguc      900

gauauccgca aagcauuaaa gcugaacgac aacacgaaau ucaaucaccu cacuuacagu      960

cauguugugg agaccaagaa gguagaaaaa gccacauuua uugagcuuaa gggguaucac     1020

cugaucaaaa agcaguugaa agacagaaac guggaacuaa cucaggagcu gcagccuaag     1080

gauuacgacg cuauagccgc ggcaucuacc uucuucaaga augauauuga auuuagggac     1140

uaucuccgua accaauaccu ggauuccaau ggaaaacgaa agagcaacau cgccaauaaa     1200

gucuucgagg acaaggugau cgcugccguu uuugagcuua acuucucgaa agugugccau     1260

cugucuuuua aguccuugua uaaacucauu ccacaccugg aaaaggguuu agauuacaag     1320

gaggcaaucc ugugugcugg cuauaauuuc cgggacgaaa acaaaagaga uaagaaacua     1380

uuucuccccg ucauucagca ggagacaaau ccugugguac acagggcccu gacccaagcg     1440

cgcaagguua uaaacgccau cauucgggag uacgggucac caaauacgau cagauucgug     1500

gucgcaaacg accuugcuaa aacuuacaag cagaggaagg aauuggagag uacacugaaa     1560

aagaaucgcg ccgaaaacga gaaaaucaag accaaacucc ugagcauggg aauuauaaac     1620

cccacuggca uggauaucgu gaaguauaaa cuuugguacg aacaguccgg uauuugccug     1680

uauacagggc gacagaugga guugucuagc cucuuuaagc cuggauacuc aaucgucaau     1740

cauauuaucc cauauaaccg gaguuucgac gacaccuacc acaaucgugu gcugacguua     1800

accgagacaa aguaugaaaa aggcaacaag auaccguuug aguacuucgg cgcugaagag     1860

acuuguugga augaauacga gaaaagaguu uccaccagca agaagaucca ugauaaaaag     1920

agggagaaac ugcuaauucg caacuuugac aucgaauaug agaaugaaau gauuucuaac     1980

aauguguucg auacacacua caucggguca uauuucgaga aguacauaga cgaacaucuc     2040

caauuuaaaa agaguacuaa aaagcagaag guauucagca uucacggaaa cauggucgcc     2100

caccuucauu cccggugggg uuuuaauaaa aacucuagag agaucuaucu uaaucacgca     2160

guggaugccg cuauuguugc gguguugacc gaccagcuga ucaggaacau agcagauuac     2220

uaucgacgcg acaauauccu cgagcagaag ggcucgcuga aauuccccga accuuggaag     2280

uccuuuucca uugaguuaga agcccggauc ucaccacaag ucagagaucu gauuaaaaag     2340

cugaacuugg acaguuaccu cgauguggac auccugcaua uaaagcccgu auucauuucu     2400

aaaaagccua ggcguaaagu gaacggggag gcucacaaga gcacaauccg cucccuaauc     2460

ggaauuaaug agaucggcaa aaccguuacu ugcauuaaga cgcggcugga agauauacca     2520

uuugacgaga acggugacuu ccccauguac gggaaggaua cagacaaaua uaccuacacu     2580

gccaucaagg aaagauaucu cgaguuuaau aaagauaagg aaaaagcauu cucaaccccg     2640

cuguacaaac uuaagaggaa cggcgagccu ggaaaucaga uuaagagcgu caagaucauu     2700

gacacacgaa acaucgugaa uaaagugaac cagggcaagg ggauagccua uaauucuaac     2760

aucauucggg ucgauguuuu uagccgcgac cagaaauacu auguggugcc caucuacuug     2820

agagauuuca uuaagggaag ucugccaaau aagaucauaa ccgcugccaa agguuuuaug     2880

caauggccug agacugacaa cagcuacaca uucuuauucu cuauuuuucc caaugaucuc     2940

aucaagguag ucccaaaaga aggcaaguuc aucaaagcaa agaacgagaa uaaagaaaac     3000

auuaaccuga cggagauacu uggguauuuu aagggacugg acuccucaac cggcgcguuc     3060

acuuuggaaa gucacgaugg uagccuccug gcuaggggga ucggcacaaa gaaucuacug     3120

cucauugaga aauaccaggu ggacguucug ggacgguaua acaagguggg caaugaggaa     3180

cgacaggaua ucaaaauuca gcaaaacuga                                      3210


<210>  2682
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2682
augaacuacg acaucggccu gagaauuggg auaaccagcu gcggaugguc caucauuaau       60

aaggaucucg agaggaucga agaccuuggu gugcgggucu ucgagaaagc cgaaaacccc      120

gauggcacag cuucugcagc cccucgccga gaggcgcgua agucaagaag gaaacaccgg      180

cgcaagaaac auagaauuga aaggaucaag cggcugauag uucagcacga cuuguuaagu      240

aagaaagagc uggauagccu auaucucucc ccauuuaaua ucgacgugug gaaccugcaa      300

guagaggcuc uugaacgcaa gcuggauaau cgagaguucg ccagaguguu gauucaucuc      360

guccagaggc ggggguuuca gacuauccgu aaaucugugg aaauucagga ggagggaaag      420

cugcuggaaa acguuucgga gaaugaacgc aucaugaaag ugaacggcua caagacgguc      480

ggugagaugu ucucaaagca cgaaaaauuu acccaucaca agagaaauaa agacgggagc      540

uauacaagug ugguaacuag gucccucuua cugaaggaga uaaaaaccau uuucaacucu      600

caacggaucc uuggaaaugu uuuuagcaca cagaaguugg aguccgauua ccuguauauu      660

uggggcucac agcgaccgac ccuaacuuac gaacagcuca ugaguauggu gggcaacugu      720

aucuaugaga aaaaggaaaa gcgcgcaccc aaaacaagcu gggccuucca auacuuuucu      780

cugcuucaga agaucaauaa acugaagguc uccgacgaua acaccuugag aaaacucuca      840

aaggaggaaa gggacauugu gauagagcug gcuuucaaga aaaagaaugu gagcuuuguc      900

gauauccgga aagcauuaaa gcugaacgac aacacgaaau ucaaucaccu cacuuacagu      960

cauguugugg agaccaagaa gguagaaaaa gccacauuua uugagcuuaa gggguaucac     1020

cugaucaaaa agcaguugaa agaccgcaac guggaacuaa cucaggagcu gcaaccuaag     1080

gauuacgacg cuauagccgc ggcaucuacc uucuucaaga augauauuga auuuagagac     1140

uaucucagga accaguaccu ggauuccaau ggaaaacgua agagcaacau cgccaauaaa     1200

gucuucgagg acaaggugau cgcugccguu uuugagcuua acuucucgaa agugugccau     1260

cugucuuuua aguccuugua uaaacucauu ccacaccugg aaaaggguuu agauuacaag     1320

gaggcaaucc ugugugcugg cuauaauuuc cgggacgaaa acaaacgaga uaagaaacua     1380

uuucuccccg ucauucagca agagacaaau ccugugguac acagagcccu gacccaggcg     1440

aggaagguua uaaacgccau cauucgcgag uacgggucac caaauacgau ccgguucgug     1500

gucgcaaacg accuugcuaa aacuuacaag cagagaaagg aauuggagag uacacugaaa     1560

aagaauaggg ccgaaaacga gaaaaucaag accaaacucc ugagcauggg aauuauaaac     1620

cccacuggca uggauaucgu gaaguauaaa cuuugguacg aacaguccgg uauuugccug     1680

uauacagggc gccaaaugga guugucuagc cucuuuaagc cuggauacuc aaucgucaau     1740

cauauuaucc cauauaaccg aaguuucgac gacaccuacc acaaucgggu gcugacguua     1800

accgagacaa aguaugaaaa aggcaacaag auaccguuug aguacuucgg cgcugaagag     1860

acuuguugga augaauacga gaaacguguu uccaccagca agaagaucca ugauaaaaag     1920

agagagaaac ugcuaauuag gaacuuugac aucgaauaug agaaugaaau gauuucuaac     1980

aauguguucg auacacacua caucggguca uauuucgaga aguacauaga cgaacaucuc     2040

caguuuaaaa agaguacuaa aaagcagaag guauucagca uucacggaaa cauggucgcc     2100

caccuucauu cccgcugggg uuuuaauaaa aacucucggg agaucuaucu uaaucacgca     2160

guggaugccg cuauuguugc gguguugacc gaccagcuga ucagaaacau agcagauuac     2220

uauaggcgag acaauauccu cgagcaaaag ggcucgcuga aauuccccga accuuggaag     2280

uccuuuucca uugaguuaga agcccgcauc ucaccacagg uccgggaucu gauuaaaaag     2340

cugaacuugg acaguuaccu cgauguggac auccugcaua uaaagcccgu auucauuucu     2400

aaaaagccua gaaggaaagu gaacggggag gcucacaaga gcacaauccg uucccuaauc     2460

ggaauuaaug agaucggcaa aaccguuacu ugcauuaaga cgcgccugga agauauacca     2520

uuugacgaga acggugacuu ccccauguac gggaaggaua cagacaaaua uaccuacacu     2580

gccaucaagg aacgguaucu cgaguuuaau aaagauaagg aaaaagcauu cucaaccccg     2640

cuguacaaac uuaagagaaa cggcgagccu ggaaaucaga uuaagagcgu caagaucauu     2700

gacacaagga acaucgugaa uaaagugaac cagggcaagg ggauagccua uaauucuaac     2760

aucauucgag ucgauguuuu uagccgggac caaaaauacu auguggugcc caucuacuug     2820

cgcgauuuca uuaagggaag ucugccaaau aagaucauaa ccgcugccaa agguuuuaug     2880

caguggccug agacugacaa cagcuacaca uucuuauucu cuauuuuucc caaugaucuc     2940

aucaagguag ucccaaaaga aggcaaguuc aucaaagcaa agaacgagaa uaaagaaaac     3000

auuaaccuga cggagauacu uggguauuuu aagggacugg acuccucaac cggcgcguuc     3060

acuuuggaaa gucacgaugg uagccuccug gcuagaggga ucggcacaaa gaaucuacug     3120

cucauugaga aauaccaggu ggacguucug ggaagguaua acaagguggg caaugaggaa     3180

cggcaggaua ucaaaauuca acagaacuga                                      3210


<210>  2683
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2683
auggccacca gacugggccu cgacaucggg acaaacagca uuggauggug ccuuuacgau       60

ggugacucca uaagggauau cggcgugcgg auuuuccugg acgggcgcga gcccucuaag      120

uugacugucu caggaagugg cacgccuuua gcuguuaaac gacgugaugc aagaggugcc      180

aggcggcgca gagacaggua uaucgggcgg cgcagcgcga ugcugcgaga acuaagaaag      240

cacggacucc ugccagcuga uccgaaacag gccaaggugc uugagaccca agaccccuac      300

gcacugaggg uacgggccuu ggaugaaaaa cucgagccuc aucagauugg ccgugcucug      360

uuucaccuga aucagcgcag aggcuucaag uccaacagga aggcagaacg ggccucuaaa      420

gcuggggagg acggaaagau cgccacagcg acuaaacucu uagaugagga aaugcagcga      480

cugggugcac gcacccuugg cgaguuuuug gugaagagag acucggaguu cgauggggag      540

gccggaaaaa ggguccggau gcgcgcugac ucaaaggccu augacuuuua cccagauaga      600

aggcaugugc uggacgaguu ccgugcaaua uggaaugcuc aaaagguuug gcaccccgaa      660

cuacucacag agcaggccug gaacgauauc uuucggguga uugaauauca gcgagagcug      720

aaaccucagg ucgugggcca uuguaccuuc gcggguauca augggguacc agccgacgaa      780

aagagacuuc ccaaagcaca cccucuguuu caacagaggc gcuuguacga ggagguuaac      840

cagcucaagg uggucgcucc aggagaagcc ggccggccgc ugacuagaga ugagagggac      900

gcuuuaauuc ugaaacucca ggauaagaaa agcgugaguu ucgaaacacu uggcaagacc      960

cugaaguuga aagacgggga gcgcuuuaau aaggaauccg agaaccgaaa agcccuagau     1020

ggagacgagg ugcgggcagc ccugucuggu aagaaacucu ucggcacgcg uugguuucac     1080

uucagcgcug aagaacaaug gaugaucaua gaaagacugc uugaggaaga ggauuccgag     1140

acucuguuga ccuggcucaa gauuggguau aggcuggacg acgaagcggc auuagccguc     1200

gcuaaugcca agcugcccga gggacauggc cgcuucgguc uaucagcaac aacugcccuc     1260

cugaaagaac uuaaggcuga uguuugcacc uacgccgagg cagcgaaacg ggcuggguuu     1320

caccauagug acuucagaac aggcgaggug uuugaucagu ugccuuacua uggagaaauc     1380

cuggccaggg agauugcacc aggcaagccc gaauacgggg acgagaacga acgacgcuuc     1440

ggaaaaauca ccaauccuac gguacacauc ggucucaacc agcugacuaa gcuggugaau     1500

gccauuauac ggcugcacgg caagccagau uauaucuuug ucgaguuggc uagagagcuc     1560

aaacugaacg aaaaggagaa aauugccuac aauaggcaau uaaaggaaaa cacagacgca     1620

gcucgugauc gcggggccaa acugggaacc augggcgugc gggacacugg uagaaacagg     1680

cuaauccucc gacuguggac agagcuuaau cccggggaug cguugaaccg gcgcuguccg     1740

uucugcggcg aacagauaag ccagagaaug auucagaaug gagagguuga gaucgaacau     1800

aucauuccuu auucucaaug uuuugacgau ggcaucggga acaaggugcu gucccacguc     1860

aggugcaauc ggcagaaggg agaccagacc cccugggagc gaugggguca ugauccagac     1920

agauggucaa uuauacagga acaaguggcc caccuccauc gcagcaaaca guggagguuc     1980

ggcccugacg cucgugaucg guacgcagcc gaggaagaca guuuucugcc cagacaguua     2040

aucgauacgc aguaucugac caggauugcu gccaaguacc uugcauguuu guacccagag     2100

cgcggggagc gacacguauu cgcgguucac ggaggcauga cagccaugcu cagacggcug     2160

ugggaccuaa acucuauccu gcucgaucau aauugggugg aaaaccuuaa uuccaacgcu     2220

cccaaaaaua ggcuugacca ccgccaucac gccaucgaug cagcugucgu ggguauuaug     2280

acuccugggu ugauagccga gaucgcaaag gccgcugcgc gugccgaaaa acaaaaccug     2340

gacagauuau uugaggagcu cccaaugccg ugggcacagc accccaauga ucggagguuc     2400

cgcgacgaac ugcgagagaa gauucuugcu gccaccguca gccauaaagc cgaucacggc     2460

agacagggaa agccugaacg gggcagggac aucacagcug ggaagcugca uaacgauacu     2520

gcauauggau uuaccggcuu gacaucggac accggucucc cagugguugu gcaccgcgua     2580

ccccugacuu cucuaacgcc uaaagacauu gccgauccag acagaauccc cgauacaacc     2640

cugagggggu cccucuggca ggcgacucgg gaccugucag gaaaggaguu cgaaaaagcu     2700

cuuuuggauu ucagucguac ccaugcccaa uuuaagggca uacgccgagu cagagugagg     2760

gagggucuga cauuaauccc gauucggaau cacgcaggca aaccuuacaa gggguaucuc     2820

ggagacagca acgcccgcua caacguuugg cagaugccag auggcagaug ggugcaccag     2880

gucaucucca ccuucgaccu gcaucaaccc ggguuuauug agcagaggcc ucaccccgcu     2940

gcaaagaaag ugcuuucucu gcggcagaau gaucuaaucg ccauagaacg agacggagcg     3000

cucagacaga uuaugcgcgu acaaaaguau ggccagaacg gucagaucuu ccugauugag     3060

cauaaugaag cugggaacau ggccgagagg aauaaaaaca cugaugaccc auuuaaguuc     3120

uacggaccug gcgcaagcgc cuugcggaau augaaagcuc gucaggugag aaucgacgaa     3180

cuggguagga uauuugaucc cggcccacgc uga                                  3213


<210>  2684
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2684
auggccaagg acuucgagua cauccugggc cucgaucuug gggaaaccag cauuggaugg       60

uccgugguca gacugaacga ggguguuccc gaacacuugg agagguuagg cgugcggaua      120

ugcucugacg ggcgcgaugc ucagucacau gaaccucuga guguagcacg acguaucgcc      180

agaggacuaa ggcggcgcag agacagguau auucaccggc gcauccgacu cacagaugug      240

cuggucaaau acggccuuau gccagaggac auuaaggaga ggaaaaagcu gcaagcguug      300

aauccguaua gacuccggag cgaagcucug gaucguaaac uggagcccua cgaacucggu      360

cgcguguuau uucauaucaa ccagagaagg ggguucaagu ccaauaugaa gacugaccgg      420

aaagauucug agucgggagc caugaagcag gcaauacuga aaacgcgagc caagauggag      480

gcuucaggcg cacgcacccu uggcgaauuu uuguauuucc ugaacaaaga caagacaagc      540

acucaggagu acguuccugu gagaaugagg agucacuccg aaaagaauaa aaccgucuau      600

gagauguacc cagaucgggc cauguacgaa gacgaggugc auaagaucuu ugagcaacag      660

gaacuaucuc ccgaggcuca gacagaaauu uucaacacca ucuuuuucca gcgcccucuc      720

aaaccaguag agcugggguu uugugaguuc gaagauggag agagaagggc cuauacugcg      780

agcguugugu uucaaaaguu ccgugcacuu cagcagauua aucagaucua cguccaagac      840

gacuaugaac ggcgaccccu gacaaaagag gaaaaggaua uauugaaaca cuuucuccug      900

gaggacuucu ccgccuuaga uaagaacggu cugcucaccu gggcuaaggu gagaacgcuu      960

cugaaauugc agaagaaaaa guucaaucua gaaucagaga ggcgcaaagg ccugaacgcc     1020

gacgugacca guuggaagcu cagcaaugca gcuugcuuug ggcgggagug guucucuuuu     1080

uccgaugcca agaaagaaga gauugucgcg cugaucacug gagccgaaaa ggaggaggaa     1140

cuuauugcag agcugaaaac aaaguacggc uugucagacg aaagagcuaa aaacacugcc     1200

aauguuagcc ucccugaugg uguggcuagu cugucugcca aggcaaugaa gaaauuaauc     1260

ccauuccugg aggaagggau gcuauaugac gaugccguaa agcaggcugg auacguguuu     1320

uccagcucgu ucaaaggcgu ccucccggag aaguaugaga ccuuuaucga acccgagucu     1380

ggcgaauguu ucgacgagcu gccuuacuac gggcaagugc uuaggcgcua uguuuuggga     1440

ggugugccau ccgaagagaa caaacuguuu cccgagaagu acuauggcaa gauuaauaac     1500

ccuacagucc acauagugcu caaccagcug cgaaaacuua ucaauucacu gguagcgaag     1560

uacgggcauc cacggaaaau uguuguggaa auggcacgug auuugaagcu caguaaaaag     1620

cagaaagacg ccaucgcuaa ggagcaggcc cugaacgaaa gagagaauaa gaggauagga     1680

gcagaauuag agcgccugga cauucggaac aauuaugaua acagaaugaa auacaagcua     1740

ugggaggacc ucagcaccga ucccacgaaa agguccugcc cguucugugg caagcaaauc     1800

ucucagcagg acaucuuuag cccugaguuc gaaauugaac accugauccc cuuuucacga     1860

aguuucaugg auggucgcuc caauaaaguc cuuagcugcc ggagauguaa cagggagaag     1920

gggaauaaga cuccauucga ggccuuuggc aacgacucua cacguuggaa ugcuauuaug     1980

ggacgcgugg aaucauugcc ugagaacaaa cgguggaagu ucgccaguga ugcaaugagc     2040

agauuugaaa gggacggcga ucugcucgcg cgaaugauga ccgacacuca guauauggcu     2100

cggauagccc gcagauaccu gugcugugca gccaauccca accaagucua cgggauccca     2160

ggacaguuaa cagcuaaacu gagggagcau uggggucuua auuccauuuu gucugaucag     2220

aaggaaaaag accggucgga ucaccgacac caugccaucg acgcauucgu gguugcuugc     2280

accgacagag gcacgcucca gaaguauagc accgagcgca gggauuacgc caagcccgag     2340

augccuuccg aaaaagcgcc acacaucccg uuugccgacu uccgucuguc agauauuaag     2400

cggguguuug acgagauaau cguaagucau aaacccgaua gagggaacgc uagggcagcc     2460

auucuaaagg gaggcacagu cgcuaaacug cacgaagaga cuuauuacgg uaugggaggc     2520

gaaggaucua agaagggcac cuggaaacuc auccaacgca uuccugugag cgagaucaag     2580

aaagccuccg accuggauug ucuuauagac cagaagggga cagcaaaccc auugcgaguu     2640

cuguuagugg gagcgucaga gagcgaacag gagcagauca uugccucuua uuucgcuaga     2700

cgggguacuc auaaagucag gaccuaccgc gaacguaaua aggaugugau ccuccccuuu     2760

aaagacaagg auggcaagcu guauagauac gccguauacg gguccaacgc auaugcugag     2820

auuuacugcc cugaccgggg gaaaaaugcc ggcaaguggc aaguggaaau cauaccaaac     2880

uauugcgcac accagaaagg uuucauuccc aaguggaggc acacagaugc ccaugcuaaa     2940

cuuaucaugc gccuguaccg aaaugacaug guugcguaug agaaggaggg gaagaccguc     3000

auugccagag ugaaaaagau gggcggaaac ggcgugaucg ucuuucggaa ucacuugaua     3060

gcaaaagaag agggggacag gcucaguugg caggcuguug ugagcggaau gcaaucugcc     3120

aaccugcgca agaucuacgu ggauguacua ggcagaguca uugacccgaa aaggccuaug     3180

acucagaaug aaacggaugg ucaggacaac uga                                  3213


<210>  2685
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2685
augagccacu ucagacugaa gcagcucaac uacauccuug gccuggacuu ggggauugcc       60

uccgugggau gggcugucgu ugagauagau accgaagaga aucccacaag guuaaucgac      120

guggguguac ggacuuuuga acgcgcagag gugccuaaaa cgggcgauuc ucuggccgcg      180

gcucgacguc aagccagauc agcaaggcgg cuaauucgca gaaggaccca ucggcuccug      240

cgcgcccgaa gacuucugaa gagggaaggg aucuugcacg cugacgaucu caacgcagag      300

ggacugguca aagagcugcc aaauacaccg uggcagcucc ggguggacgg cuuagaucgu      360

aagcugaugc ccaaagaaug ggccgcuguu cuuuugcauc uggugaagca ccgcgguuau      420

cuaagucaga gaaagaacga gagcgaaggg gccgacaaag agcucggaag gcugcuugcg      480

ggcguccaug agaaucacca ggcacugcaa uccucugaau accggacucc ugccgaguug      540

gcugugaaga aauuccaggc ccagucgggc cacauucgaa accagcgcgg ggauuauacc      600

cauacauuug acagaaagga ucuccaacag gaacugcacc aguuauucgc agcucagcaa      660

aaauacggaa auccauuugu aucagacggu cugaagcagg gcaucgagac ccuccuuaug      720

cagcaaaggc ccgcccugag cggggaugcg guucagaaga uguugggacg gugcacuuuc      780

gaaacagagg aguuuaaagc cgcaaagcau accuauagug cugaacgcuu cauauggcua      840

acgaaacuga acaaucucag aauccaggag aacggcaaug aaaggugucu gacugcccag      900

gagcgugguc aaauuaucga ccuuccuuac aagaaagcua agcugaccua ugcccaggug      960

cggaaagaau ugucccuccc agagaacgca uuuuucaagg ggcugcgaua cggaaaggag     1020

aaugccgaaa cagcugcguu aauggagaug aaagcauacc acgccauuag aaaggcucug     1080

gaaaaagccg gccuaaagac ugaguggcag ggccucgcau gcaaacccga acugcuugac     1140

gccaucggga ccgucuuuuc uuuguauaag acagaugagg acauaagcac caggcugcag     1200

ggacaacucu ccgaggaugu gauuaacgcu cugcuugccg gucugaauuu cgacaaguuu     1260

auccaguugu cacucaaagc acugaguaag auuuuaccuc ugauggaaca gggccagcgc     1320

uacgaugagg cgcacgcugc ccuauaugug cggagcacgc cacaaccgca gagacuccug     1380

ccccagauca cugcagacga aaucuguaac ccugucguuc uuaggacauu gucucaggcc     1440

cgcaaaguga uuaacgcuau aguacgacgg uacggguccc cagcccguau ccauauugag     1500

accggaagag aggugggcaa gucauucaaa gauaggcaag aaauccagaa gcgccaggag     1560

gacaaucaca aggaacggaa acaagcagcu gagaaguucc uggaauauuu ucccaacuuc     1620

gucggucagc cuaaagccaa ggauauacuc aaacugagau uauaccagca ucagcacggg     1680

caaugccugu auagcggcaa gacucuugac auuuguaggu ugaaugagaa gggauacgcg     1740

gagaucgauc augcccuccc auuuagucga acaugggacg acucuuucaa caauaaagug     1800

cugguucuag cuggcgaaaa ccagaauaag gggaaccaga cccccuacga guggcuggga     1860

ggugcaucca auagcccgcg cuggcaggaa uuuguggccc gggucggcuc gucuagauuc     1920

caccacucca aaaagcaaag gauccuccug cgucagcuug augaggaagg guuuaaagag     1980

cgcaacuuga augacacgcg guauguggcu agauuccugu gcaaguuuau ugaggaaaac     2040

auguuacucc ugggaagggg caaacgacgg guauucgccu caaaugguca gaucaccagu     2100

uuucuucgca gcagaugggg gcugaggaag guucgggagg aaaacgaucg acaucacgca     2160

uuggacgcgg uggucguggc cuguuccaca gucgcuauuc agcaaagaau aacuuacuuc     2220

gugcgccaga gggagaugaa uaucuuugau ggcgaacuca uugacaagga gaccucugag     2280

guuagccguc cuuauuuccc ccagccaugg gauuucuuuc ggcaagaagu gaugaugaga     2340

guauucggaa aaccugacgg cgaucccuca uuuccagagg ccgacacaaa ggaaacucug     2400

ugcacccuac uggagacaag gcucaguucc cgcccccagg caguccauga guacgugacc     2460

ccucuguucg uuagccgagc uccaacuaga aaaaugucug ggcagggaca caaggaaacg     2520

gugaaaucag ccaaacuuuu gcacgagggu aagagugucc ugcgggugcc guuaacacag     2580

cucaaaccca accugcuuga aaagauggua aacagggauc gcgagccuca acuguaugaa     2640

gcacuaaaag agcgucucgc cgcuuacggc aaugacccag cgaaggccuu ugcaaccgag     2700

uucagaaaga agggagggca gcuggugaag gcuguucggg ucgaagauac ucagaaaagc     2760

ggcguguugc ugcauaaggg uaccggggug gccgacaacg ccacaauggu caggguugac     2820

guguuugaga aauccggcaa guauuacuua gugcccaucu acucuuggca gguagcucaa     2880

ggaauccucc cuaauaaagc agucauucag ggcaaggaug aaaagaacug ggacgagaug     2940

gaugaaaccu auacuuucaa auuuucgaug uacccaaaug accuggugga gauaaacaca     3000

gggaagcagc gccuuuucgg auauuacauc ggccuggauc gagccacggg ugcgauuagc     3060

aucagagagc acgacuugga uaauuuuaaa gggaagaacg gaauuuaucg gguuggcgug     3120

aaaaccgcua ucuccuucaa gaaauaccag acugacgaac ucgguaaggu cgugguuccc     3180

uguaggccga agaaacgccu gccugugaga uga                                  3213


<210>  2686
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2686
augagacugg gccucgacau cgggaccaac agcauuggau gguggcuuua ccagacagau       60

gacgguacua uaacggaugu gcuggacggc ggggucagga ucuucuccga uggacgggac      120

ccccaaucua aggagucauu ggccguugau cgccgagugg cucgugcaca gagaaggcgg      180

cgcgacagau auuuaaggcg gaaagccgcg cugaugaagc gcauggcugg cgccggucua      240

augccuaccg aucaggcaca cgccaaaaag cucgaaaguc uggacccaua cgcucuuaga      300

gcagaggggc uggauugcga auugccgcuc acacaucugg gacgagcccu guuucaccuc      360

aaucagaggc ggggcuucaa aagcaaccgu aagacugacc gcggcgauaa ugaguccggg      420

aagauuaaag acgcuaccgc cagauuagac ugggcgauga gggaugcaaa ggcccggaca      480

uauggagaau uucugcauac ccgacaacag aaagcugacg aucccaagcg caugccuacu      540

guaagaacaa ggcuuaccgu ggcccggcgc gacaacgcag agaaagagga agcugguuac      600

gauuucuauc cagacagaag gcacuugucu gaggaauuug augcccugug ggcggcccag      660

gcagacuacc augaggcucu aucggaugac cuccgugauc agaucaagac gauuaucuuc      720

caccaacggc cccugaagac uccugagguc ggccuuuguc uguuuaccga cgaacgaaga      780

auaccaucag cccaccccuu gaaucagagg cgcauccucc uggagacagu gaacgcuuua      840

cggauuguug ccagagggga agcagccagg cgccugacuc ucgaggaacg agaccagauc      900

gugcaugcuc uugauaauaa aaaccacacc aagacacuga gcggaauggc gaugaaauug      960

aaggcacuag gcaaagucau ucggcugcgu agugaccaga ccuucacgcu cgagacugcc     1020

aauagagaug cuaucgccug cgaccuggug agggcauccc uuucucaucc ugaucgcaug     1080

ggugggguau ggacaacccu ggacgaggga gcccaauggg aaguugugca gcggauaaga     1140

gcuguccaga gcgauuccga guuugacgcc uugguggugu ggcucgaaca aaaccacggc     1200

cuggauaaug cacacgcgca ggcuacugcc aacgcaccau uaccggaggg cuaugggagg     1260

augggacuga uggccacacg acgcauucua gcugcccucc agucagacgu caugcccuac     1320

agugaugcag uugcugccug ugguuggagc cauucuggcg ggccuaccgg agaagugcug     1380

gagaaguugc cauacuaugg caaaaucuug gaccggcacg uaauucccgg uacguacgau     1440

gagaauaacg aagacgugaa gagauauggg aggaucacca auccuacagu ccauaucggc     1500

cugaaccaga uucgucgccu cgugaaccgg auaaucugca uucacggaaa gccagacgag     1560

aucguugugg aacuggcgag agaucuuaaa aauuccgagg accaaaagag ggaaauacag     1620

aaaauuauca agaaaaacac ugaugccgcu aucaagcgag gcgagaagcu gauuaaugag     1680

uuggggcaga aagacaccgg agcaaaccgg cucguccugc gcuuauggga agaucugggu     1740

caugacguga ugacaagaaa uugucccuac ucaggcaaga ggaucagcgc cgggaugcua     1800

uucgauggaa guugcgacgu agaucacauu cucccguauu cucggacucu ggacgauggc     1860

auagcuaaca aaacccuuug uuugaaggag gccaaucgac agaaauccaa caauacaccu     1920

ugggaaguuu ggggugagac cgagcaaugg gacacuaucg cagcgaaccu gaagaaucuc     1980

cccgauaaca aggccuggag auuugcuagc gacgccaugc agcgcuucga aggggagaau     2040

gacuuuucgg caagggcucu gaaagauacg caguacuuau cucguauugc ccgggcauau     2100

cuggacgccc uuuacgaugg cggagacggc aacucccacg ugugggucgu gccagggaga     2160

uugacagaaa ugcucaggcg ccauugggga cugaaugguc uagaggcucu gaccgauuca     2220

gacgaaaaca cugucaaggc gaaaaaccga auggaucaca gacaucacgc caucgacgca     2280

gcugugguug ccgccaccga ucggggccuc auccagagga uuaguaagau agcuaaacgc     2340

gacgagcaag caggggccga ggaaguggcg cguagcguau ccccucccug ggauggauuc     2400

agagacgaca ucgcugccca gcugucucgg auuaucguca gccacagggc agaucauggc     2460

cgcauugacu cacaggccaa gcagcaaggu gaggaugcua cagcagggca gcuucacaug     2520

gacaccgccu acggcguggu ugaugcggga cgagugguca guagaaagcc auugcugucc     2580

uuaagcgaau cugacaucaa aaagauacgg gaucccgacc uccagaggca ucugacucgc     2640

gugacaaaag gccuugauaa gaaagaguuc gaacacgcuc ugucagacuu ugccaaugca     2700

agaaaguugc cugaucaaag ugagaaccca uauuucggga ugaggcgggu acgucucauc     2760

gacgcccugc aggauagcgc ucgcgugucc guucgagaca gagacggaca uauuuacaaa     2820

gccuauaagg caggcucuaa ucacugcuac gaggucugga ggcuaccgga ugguaagauc     2880

aaacccuggg cuauuucgac guuugaagcc caccagaccg ggacugagag caagccucau     2940

ccagcggcca aacggcugcu ccgcguguuc aagagagaca ugguggcaau cgaaagggau     3000

ggagagacac ugauguguua uguccagaaa auggacauag cuaacggccu uuuucuuguu     3060

ccccacaccg aagguaaugc cgaugcucgg aaccgagaca aggaugacuc cuucaaguuu     3120

auucaaaugu cagcagugag ucugaucaga gccaaaauuc gcaggaucca uguggaugag     3180

augggccggu uacgugacca ggggccucca agauga                               3216


<210>  2687
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2687
augagauaca ccaucggccu ggacauuggg auagccagcg ugggaugggc ugucaucaac       60

gaggauaagu ccaggauuga agaccucggu guucgggugu ucaaaaaggc agaggaaccc      120

gauggcaaau cucuuaaucu ggcccgccga gaggcgcgug ggucaagaag gcggauccgc      180

agaagggcua cacggaugca gaagguaaaa gaauuguuag ugaaguauaa ccuggucagu      240

gagaaggagc uagaaacucu cuacauuauc acggagacca caccugacgu guaugaacug      300

cgcgccaaag gacuugaucg acuguugagc agacaagagu uugcaagggu ucucacuaau      360

auagccaagc ggcguggcua caaauccaac cgcaagaucg acgaggaaga uaaagaggug      420

gguaagcugu cuaaggggac cauugaaaau aaaagaaucc ugcaggagaa gggauauagg      480

acaauuggcg aaauguucua caaagacgag aaguuugagu auaacaaacg gaauaaggcu      540

ggcgaauaca acucgaccgu ccuccgauca auguuaaucg augaggugca cacucuguuc      600

gaaaugcagc gcaaauuuaa gagcgaguuc gcaaaugaag aguuugagaa ggaauacgua      660

gagguuauga cauaucagaa accauacaua accccggaac uuuuggagaa gaugcugggg      720

aaaugcacgu ucgaaaaggg agagcccaga gccccuaaaa acaguuauac uuuugagagg      780

uucaugcuac uccaaaaggu gaauaaccug aauauuuaca ucaacgguga aaaggucgag      840

auuccacagg agaaaaagaa agaaaucauc gagcuugcuu auaagcaguc cgaaauuaaa      900

uacacccagc ugcggaagaa guuggagcuc cccauagaag cccgcuuugc ggaccugaau      960

uaucaaauca aaagaacaga ggaugaaucu gaggaggcau uagugaagaa aauugaagac     1020

aagaaauucg ugaagcugga gggcuggcau aagaucaggc ucgcccuuaa agaaaacaag     1080

gauaaauuug agaagauaga gaaaaacccu gaacugcaga auauuaucgc ugacgccuug     1140

guccguaaca agacugacga gaccaucaaa aauuaccuag aaaagagcga uauugacgag     1200

gaaaucauua acgcaauacu gaauaucaac uucacaaagu ucgggcaccu cuccuacaaa     1260

gcuaugaaua agauuauccc agagcuggaa aaaggacuua ccuaugauaa ggccugugag     1320

aacguuggcu acgaguuuaa aggucggaag aaugaacugc aguauaaguu gcccccugug     1380

acggagcucg aagacgagau ccugaacuca guaguguuac gagcggucag ucagacuaga     1440

aaagugauua augccauaau cgauaaguac gggagcccaa cagcaauuua uaucgagacc     1500

gcuagggaac ugucuaaacc guacgacgag cgcaugaaga uugaaaaacg gcaaaaggag     1560

aacgccgcua auaacgauca gaucaaggaa uauauaaaag agacuuucgg auuugacccc     1620

aagccuuucg auaucguuaa aaugaagcua uggagagagc agaauggcaa augcgccuac     1680

uccucaaagc caauucccgc agaaaggcuc uacgaggaaa acuuugugca agucgaccau     1740

aucauuccuu ucagccgcug uuuugaugac aguuaucaga acaaggugcu gguacuuaca     1800

gacgagaauc agaaaaagaa agaacgaacc ccauucgagu acuuuggcga ggauaaggaa     1860

cgguggcacc aguucgagga auuuguuaac uugacguaua aauucaauac ccguaagaaa     1920

gagaaccugc ucaucaagaa uuuuaacgaa gagaagucua aagaguggau acaaagaaau     1980

auuaacgaca caaaguacau cuccaaauuc auguauaauu acauugccaa caaucugaag     2040

uucgcugaua gcgaaucgaa aaggaaggug uauaacauca augggcaggc gacuucuaua     2100

cuucgccauu acuggggacu gaagaaagac cgggaggaau ccgauaagca ccacgcacag     2160

gacgccguca ucauugcuug cgccaccaac aaaaauauca agaaaguguc agauuacagu     2220

agaaggaagu ugcucuauaa gaacaacgag auuaucgacg aggaaacagg ugagauaauu     2280

gauaaaaagu acaauaucga caucagcauu aaagaacccu ggccgaaguu ucgagaggaa     2340

gucgaggcac gcauggagga ugccgacaac aauggccagc uguaugcuuu aaucaacggg     2400

aauuucacua acuacgauga cguggacauu acccauauaa aaccuaucuu uacaucccgg     2460

augcccgaaa gaaagauuac cggaagggcc cacaaggaua cuaugcguuc ucaaaaauuc     2520

auccagcagg gccagaauuu uacgguugug aagaaaccac ugaaggcaau uagcaaacaa     2580

gagaucgaag guauaaucaa caauccugac uucaagaacc uauaucucuc agauaagaaa     2640

cuguacgacg auauuuauca gaagaugaaa gaggcggacu uuaaggcuga aaaagccuuc     2700

gcaaaugagu accgcaagua caguaaaaac ggcaauuccc ccaucguacg gagcauuaag     2760

gucgccucug cuggggccac aggagugcag cuuaagaacg gcgcaaucgc ugagaaugcc     2820

aacaugguua gaguggaugu cuuugaaaaa gacaacaaga auuauuuggu gccaauauac     2880

guaucagauu ucgcgaaaaa cgagcugccc aauaaggcca uuugugcuaa caaagacgaa     2940

aaggagugga ucgaaaugac cgagaaguau aaauuuaagu ucagucucua cccuaaugau     3000

cuggugaaaa ucaagaggaa aaacgagaag gaauuuuaug cauacuauug cagcacucau     3060

cgagggaccg gaaauauuaa cuuacugcuu guuaauggug aggcccggaa ggacggcguc     3120

gggaucaaaa acacagaaau auucgagaag uaccaggugg auguguuggg aaauauuucc     3180

aaagucaaca aggagaaacg cgaaggcguu aaguga                               3216


<210>  2688
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2688
auggacuaca gaaucggccu ggauauuggg accaacagca uaggaugguc caucauugag       60

cucacaacug ugcagucuaa acuugacggu aaauauaggc ugaaggccgu cggcuugauc      120

gaugcugggg uucggauguu cgaccgcgca gaagugccca aaacgggagc cucauuagcg      180

cugccucgac gucuagcuag aaguagcagg cggcgccuca ccagaagguc cgcccggaag      240

aaagcaauuc gcaaucugcu uguaaaggag gauauccuga accaaauaga auuggaccac      300

cucuaccgaa caucugagcu gguggauauc uggcagauua gagucgaagg ccuggagagg      360

cggcucgguc gucaggagug ggcccgcuua cugauccauc uugggcagaa gagaggauau      420

aggucgacuc ggaaagcuga agagggcgca gacaccgaag gccgagccgu guugucaagc      480

auuaaggaga aucaagagac acugaaacag uaccgcaccg uuggggaaau gugggcuacu      540

gaugacaagu uuaaaggaag agugaggaac aguacaaaug aguucaucuu uaacgucucc      600

cgggauaauc uagaagagga aauacgcuuc cucuuucagc agcaaagaga gcuggguucu      660

auguaugcca gcaaacuucu ggaggaaucc uacauggagg uguggggcca ccaguugcca      720

uucgcgucag gggaaaguau ucucaagaac guaggaaccu gcagccugua ugagaaggaa      780

aagagggcag ccaaagcuac guacacuuuu caguacuuca uggccuuaga caagcugaau      840

aacguucgua uccucccguc uaugcggacc cuuuccgagg aggaacgagg ccuggcauug      900

aaagcuaugu uugagagaga uaagcuaccc aaaaauccuc caaaguucac auauucacau      960

cugaggaagg ccauuaaacu cgaagaggac gaaacuuuuc agaccaucag cuacgacgug     1020

gagaagacac uggcgaccaa cgagagugcc gucuucguga aucuugcagc uuauuaccaa     1080

aucaaaaagg ugugugauga acacaacgag aauuaugacu acgucgauua uaacacgauu     1140

ggucaugccc ugacuguuua caaaacagac gaagauauaa acaaguacuu ggcuucuaag     1200

aauaacaucg ccaaacgcgu guaugcaaau gacuuuauug auuccguacu caagcacagc     1260

uacaccacuu ucgggcaccu gucguuaaaa gcccugucuc ggaucauacc ccuaauggag     1320

gagggagaag uguucgcuaa ggcgaaagag aaggcaggcu uuaaggaaga guccuaucuc     1380

gaaauuaaau caaagcugcu uacaaacauc ccugacgcca ucaaaaaucc agucgugaug     1440

agaaguuuga cccaggcuag gaagguuauu aacgccauca uuaaacgcau gggcgagcag     1500

cccgcaagca uacaaaucga gcuggcccga gaacucucca aggauguguc ugagcggaag     1560

aaaauuacga auggguucgc ugaaaaccgu agcagaaauc aguuugccau caagacccug     1620

gaggaaaacc auaucacaaa uauuagggga caggacauag ucaaauucaa acuuuggcag     1680

gagcaaggug gccgcugcgc auacucacug aagccuauca aguuggagga acucauuagu     1740

uuucugaaaa aggagcggag auaugaacac aacuuagaug uggaccauau cauuccauac     1800

uccaagacuu ucgacgauag cuauaccaau aaaguacugg uguuuucuga cgagaacagg     1860

aagaaagggg aucagauccc ggcggaauac cuaggaauag gcacaaagcg augggagaaa     1920

uacaaggagu ucguuaucac uucaaaguuu cccgcuagua aaaagaaacg ccuccugaag     1980

gaacagcuug ucgagcggga agaguuguuc augaaagaga gacaccugaa ugacaccagg     2040

uaugccagcc guuacuuuau gaacuuccuc cgcgaaaacc uguuacggaa ggagacagug     2100

cugcacgaaa cccagugggu cacugcagug gccgguagag uuacguccca uauuaggaag     2160

cgacuugggu uuggcaaaga ucgggaggaa acacacuugc aucacgcucu cgacgccauc     2220

auuguggcau guaccgauaa guggaugauc gacugcguaa cuaauuauua caaaucucgc     2280

gagggaggcu cgauaaagag aaaagagauu agguucccua gcccauggaa cggguucugg     2340

caugaugucc aaaccaagcu ggcucagcua ccccagccug aagugaucaa ugaguccauu     2400

ucaaagggaa acccagacgc cguugauuau augcugguga gucggaugcc ccgaaaaucu     2460

aucacaggug cggcccacaa ggaaaccaua agcaaauggg gcggguacga cgauaaggga     2520

aaagcucucc uggucaagaa aguggcacuu uccgaguuga aguuuaagaa ugacgacuuc     2580

ccgauguaua acaaagaaac ugauccucug uuauacgagg ccaucaagaa uagauacgua     2640

gagucaggcc gcgacgugaa aagggcuuuu gccacacccc ucuauaagcc aagcaaaucu     2700

gguaagcgua acgaaguuaa gcgggucaaa gugauugcag agaagaaagc gauggugaga     2760

gaagucaaug ggggcaucgc cgagaacgga ggccugguua gguccgauau uuuccuuaag     2820

gaaaagaagu acgugauggu gccuguauau acguuugaca ccaucaagca gcaacugccc     2880

aauaaauacg ucgugagugg gcgcggauau gagcaguggc cagaguugac ugcugaacac     2940

guuuuccagu uuagcgugua ccccuaugag cucguccaug uggguacaaa caagggcgau     3000

aauucuuucu acuacuuugc cucccugaac auaucaagua auguucuaaa auucagcgaa     3060

uguaaccgac cuaccaagga cuauauugag aucgggcuga acucgaucau ucuccuggag     3120

aaauacgaaa ucgaugugcu uggaagaauu acaggccagu ccaagaaggg uauacggcaa     3180

ucuuuuaaaa auaacaagag ccagaugaaa gaguga                               3216


<210>  2689
<211>  3219
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2689
augagacugg gccucgacau cgggaccaac agcgugggau gguggcuuua caagacagau       60

ggugaggccg cuuccgcaag gauugucgaa guuauagacg gcggggugcg gaucuucucu      120

gauggacgcg accccaaauc aaagacuagc cuggccguag aucgacgugu ggcgagagcu      180

gccaggcggc gcagagacag guauuugcgg cgccgagcaa guuuaaugaa acugcuagcc      240

agaacgggcc ucaugccugc ugauccaguc gaggcaaaga aacuggaaag gcuugacccg      300

uacgagcugc gguccaccgg uuuggaugac gcccuccccc ugacacacuu ugggcgugcu      360

cuguuccauc ucaaucagcg cagaggauuu aagucuaaca ggaagacuga ucggggcgac      420

aaugaaaacg gcaaaauucg agaggccacc gcgcgcuuag aucuggagau gaugacaucg      480

ggggcaagau cauauggaga guuccuucac augaggcggc aacgcgccag cgacccuaga      540

caugugccaa guguuaggac ccguuugucc guggcugccc gggguggccc cgacgcaaag      600

gcugaagccg gguacgauuu uuauccugac cgacaccauc uggaagagga guuccacgcg      660

cuaugggccg cacaggcuac ucaccaucag gcccucacag augaccugag agaucuuguc      720

uuugagaaaa ucuucuacca gaggccacug aaggaaccca aaauuggauu gugccucuuu      780

accgcugagg aacgcaucgc caaggcacac ccucugacgc aacggagagu guuauucgaa      840

acuauaaauc agcugagggu aaccgccgac ggccgcucua cacgaccacu cacucuugag      900

gagcgggaua cccugguuca ugcuuuggac aacaagaaac cgacaaagag caugaccucc      960

augaauauga cgcuaaaagc gcuggcaaag cuccugaaac uucgugaugg ugaaagauuu     1020

acucuggaga caggggugag ggaaaacauc ucaugugacc ccguccgcgc cugcauggcu     1080

cacccugccc gguucggacc acaguggagu agcuuggauu gggaggcaca gugggacgug     1140

auuucuagag ugaggcaagu ccaguccgaa ggcgaguuua ucgagcucac cgacuggcug     1200

auucagggcc acggguuaga ugccgaccau gcuaaggccg uuggaaaugc accccugccu     1260

gaagguuaug cgcgacuagg cuucucagcu acugagcgca uccucgauca gcugaaagcc     1320

gaagugugua cauacagcga cgcaguagcc aagugcgggu ggcaccauag ugaucaacgg     1380

accggagcuu gucuugaggu guugccauac uauggcgaag uccuggacag acacgugaua     1440

cccgguacgu acgaugccac cgacgaugag auuacaaggu augggcguau cacuaacccg     1500

accguucaua uuggccucaa ucagcugcgc cggcuuguga acaagaucau cgagguccac     1560

ggaaaaccug accagauugu gguagaacug gcaagagagu ugaagcaauc ugaaccccag     1620

aaaaaggaug cuaugaaaag gcuccgaaau acaacugccg acgcgauacg gcgcuccgag     1680

cagcuggagc aguuaggcca accaaacacc gggcacaaua gaaugcugcu aaggcucugg     1740

gaagaucugg gaccugccau cgguccccgg ugcuguccau acacaggcaa gaccauuagc     1800

guuacuaugg uguucgacgg gucgugcgac gucgaucaua uccuucccuu uucucgaacg     1860

uuggacgauu ccuucgcuaa cagaacacug ugucucaagg aggcaaaccg cgaaaaauca     1920

gacaggaccc cuugggaggc cuggggaggc acugaucagu gggacgauau agcugccaau     1980

cugaagaacu uaccagagaa aaagcguaaa cgguuugguc cggacgcaau ggaaugguuc     2040

gagggggaaa augauuuucu ggacagagcg cuuguggaua cccaguauuu ggccaagauu     2100

aguaggaugu accucgacac acuguucacc aagggcggac acgucugggu gguucccggc     2160

cgcaugacug agaugcuacg aagacauugg gggcugaacg cucuccugaa ugauaaagac     2220

cggggagccg ugaaggcuaa aaacaggaca gaccaccgcc accaugcaau cgaugccgca     2280

guagucgccg cuacggacua ucgucuuaug cagaagauca gcagagcggc cggucaaggc     2340

gaaaaugcag ggcaguccgc ugaguugauu gcccgggaua ccgacgcccc uugggaacac     2400

uuuagggcug auaucgcauc ucagcuggag aaaauuauag ugagccaucg cgccgaccac     2460

ggacgaaucg auguugaggg cagaaagacu ggucgggacu caacagcggg gagguuacau     2520

aacgauaccg cuuacggcgu gauugacgau cucacagucg ccagucgcac uccacugaug     2580

ucccuuaagc ccagcgacau cgcaaucacc gaaagaggca aaaauauuag ggacccucag     2640

cugcaaaacg ccuuguacgu ggcuacgaag ggaaaagagg ggaagauguu cgaacaggca     2700

cucguagauu uugccucuaa agagggacca uaucagggca uacggcgugu gcgccugguu     2760

gaaacccuac agaaggcggc ucgagucgag aucgguaaaa cagagucagg gcaaccccug     2820

aaggccuaca agggagacag uaaucacugc uaugaacucu ggaaacugcc ggauggcaag     2880

auugugccuc agguggucac uaccuacgag gcacacgccg guagcgcucc aagaccccau     2940

ccugccgcaa aaaggauccu ucggguuuuc aagaaagaca uggugaaguu gacaaaggau     3000

ggcgaagaga uuguggggua uguacagucc augcacauca aaaacggacu guuugucgcu     3060

caacauuuag aggccaaugc ggacgcccgc aacagagaua agacugaccc cuucaaauuu     3120

cuccagaugg gcgcagggac ccuggugaag acgggacuua ggcggguucu gguggaugaa     3180

auaggccgac uaagagaccc agguccuccc aaacucuga                            3219


<210>  2690
<211>  3225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2690
augcagacca agaaccugcc cuacacacuc ggccuugaca ucgggauggc cagcguggga       60

gcugcacugu uguuaccuga ucaagagagg auucuggccc uacacgucag aacuuucgac      120

aaagcggaaa cggcuaagga gggugaaucc cucaauaaaa uacggcgcga uucucgacug      180

acccguagaa ggauccggcg cagagcccau aggcuuguuc ggcuggcacg cuugaugaag      240

cgagugggcc ucauugccga cgcuaaccca gaggcauuug cccugccggg gguaucaccc      300

uggcaccuga gagcugaugg acucgacagg uuacuggaac cuaaagagug ggccggcgug      360

cuuuaucauu uggucaagca ccgggguuuc cagaguaauc guaagagcga ggcgaaagca      420

gaugaaaagg ccgggcagau gcuguccgga gugggccgca accaggcucu acucaaagag      480

gccggcuaca gaacaguugg ggaacuggca gcuaggcaug aggacuuuaa ggaggccaaa      540

cggaauaagg gagguucuua uucgcacacu uucgcgcgag ccgaucuuaa cgcagaacug      600

aauuugcucu uugagcgcca aagagcucug ggcucaaggu uagccagcgc ugacuuccag      660

gaaaacgugc gggaucugcu cauggcccgc agaccaaccc uuaguggggc aaaucuguug      720

aagauggucg gaaaaugcac auuugagaag uccgaagaca gggcccccaa agcuggcuac      780

cgugcggagc gguucgugug gcuagguaag cugaacaauc ucaaaaucgu agggucugga      840

gaugcacgac cucugaccga cgccgagcac agaauuguua ucgaccuucc auuuacucag      900

gcuaagcuga cauucaaaca ggugaggaag gccuuggaac ucgagaccca ucaacgcuuu      960

aaccuguuaa gcuaucggcc cgauccuaag ggcaaagaca aggauccaga agaguccacg     1020

uucuuugaag caaaaucauu ccacacucug agaaaggccu acgagggcgc ugggcuaaag     1080

aaggaguggc agagggacgc ccucgauccg gaccgccugg augcacuugc guaugcuuug     1140

accuguuaca aggacgauac agaaagucga caguggcugg ucgcccaggg aauagguacu     1200

cccaucauug aggcagugcu cgccgagagc uucgacaaau uuauccaucu gucucaaaag     1260

gcucuuaaag ccauucugcc ucacauggaa gcaggccagc gguacgauga cgcugccaag     1320

uccgcggggu augaccacuc acagccagau gccggaaccg gcaaacgugc uacauugccc     1380

ccuccagaca aggauaccau cagaaauccc guggucuaca gggcacucaa ccaagcccgc     1440

aagcugguua augcuauagu ggccgaauau gguccgccug cagcgguaca uauugaguua     1500

gcccgggacc ugagcaaacc caucgaugag agaagggaca uugaaaaggg gcagaaagag     1560

uuccaggcuc uaaaggaaaa acucgccgca gcuuuugagg agacguucca ccgaaagcca     1620

aguggccugg aucuuuugaa guaccgccug uuucgggaac agcucgacca augcccuuau     1680

ugucagaaag cccugguggu cgauagauua uucgagcccg gauacgcaca gaucgaccau     1740

gcccugccau auucuagguc cuuugaugac ggcaugaaca acaagguggu ugugcacguc     1800

gcuugcaauc aggauaaagg gaaccguacu cccuacgagu uccuugacgg agcgggugac     1860

agcgaacgcu ggcaacgguu uguggccugg guacagggca auaaggcaau cagacaggcu     1920

aaaaggaacc gauugcuccg gguuaauuuc gggacagaug aagccaagga guuuucgaaa     1980

cgcaaccugu cugacaccag auacauuugu agggaguuca agcggacuau agaaacacag     2040

uuugccuggc auuccgaugc ugagggaaau gaacgaugcg ugguccuauc aggccaacug     2100

accagucucc ugagagcacg cuggggucuu aucaagguga gggaggacgg ggauuugcac     2160

cacgcccugg acgucgcggu gauugcugcc gcaagccguu ccuuaguuaa acggauggcc     2220

gauuauucua gaaggaacga acucgagaug gugcgcaauc gauacguaga cccugcuacg     2280

ggcgaggucg uggauguugc agcccugaga cagaucgaag accauuuccc aaagccgugg     2340

ccccuuuucc gggaggaacu guuggcgagg cucgcuccug agccaaaacu gcagcuaacc     2400

ugugugagcu cauaugauga aggacugcgc gagaguauac gucccauuag agucucccgg     2460

gccccuacaa ggcgcggccu cggggcagcc caccaggaga cuauccgaag ccugagagac     2520

ggaucucaau cagcuaucaa gaccccacuu cgggauuuga accugaaaga cuuagaaaau     2580

auuguggguu uugagaggga cacagcccuc aucgaugcaa uucgcacuag acuggaggcu     2640

uucggcgggg aaggaaagaa ggccuuugac gauaaacagc ccccgcuuag gaagccuagu     2700

aaagacccag auaaggcgcc cauaauccgg agcguaaaac ugcuagugac ccagaagucc     2760

ggcauuccua uccguggugg gaucgccaac aauggcgcaa ugcuccgcuc ugacauuuuc     2820

acaaaggcug gaaaauuuua cgccguuccc auauaugucg cugaugcagu gcgaggcgag     2880

cugccacaua gagccguggc ccaagcuaag ccugaauugg aguggcccau gauggacgaa     2940

ucguaccagu uccuguuuag cuuacaccca aacgauugga ugaccaucag gcucaaaaau     3000

gagguccggg aaggguauua cgcgggacug gaccgcgcaa cuggcaacau uucccuuugg     3060

cugcacgaua gaaaccagaa uguuggucag aagggguugc ucacgggaaa cggcaucaaa     3120

acagcccugg cuguggagaa guaccaugug gaccuacugg guaaacuuca caggguacau     3180

aaagaggaac ggcaaccgau ucacaucaag ccuggcaaac gauga                     3225


<210>  2691
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2691
augaaguaca gcaucggccu ggacauuggg auagccuccg ugggaugggc ugucaucaac       60

gaggauauug aaagaaucga ggaccucggu guuaggauuu ucgauguggc agaacagccc      120

aaaaccggcg ccucucuuuc aacaccucgg cgccuggcgc gaaguacucg uaagagauug      180

aggcggcgca gacacagggu agagcgggug cgcaaauuua ugauccaaaa gggguuacug      240

acgcaggucc agauggaaca gcuauaugac uggaaagaug gagaccucga uauauggcug      300

gugcgaguua augcucuuga gagaaagcug agcgacaggg aguucgcccg gaucuuggug      360

cauuuugcaa agaaccgugg cuuccaaucc aaucgcaaau cugaacucca ggaggaagau      420

aacggugccg uccugucggc ugugcaggag aauaagagac ugauggagga aaaaggguac      480

cagaccauuu cagagaugcu cuuauaugac gaaaaguuug agggaacaaa aaggaacaag      540

ggcggcgaau acacucacgu aguugcacgg agcgaucuga agcgagagau ccaacuuauu      600

uucgagaaac agcgcgccua ugggcaugcu uuugccacca uggaaaauga ggacaaguuc      660

uugcagaucu ggaguuccca gagaccauuu gcgacaaaag augacauagc aaagaaaauu      720

ggaaacugca ccuucgaacc gaaggagaaa agggccccca agucuacuua cacauuugaa      780

cgguuccgcg cucuggacaa gcuaaauaga cucaggaucc ugagcgguac cuccccugcc      840

cguucacuua cgcaagagga gcgggaucug auuuugaaca gucucuuuag caaaaaggaa      900

gugaaauaua aggagcugcg aaaauuacug aagcucaaug acgaacagag auucaacgag      960

aucuucuacg auccaaauga aaagaucgag aaaaacgaga auaggacuuu ucuuucucug     1020

gaaucccagu acaagauucg caaaauauug caaaagaucg agggcaaauc acuaaacgac     1080

accuaucggc ccgucgaucu ggacacaauu gcauacgcuc ucacuguguu caaggaugac     1140

caggauauca gagacuaucu gcagaaccag uacguggcca gcaaugggaa gaaagaacuu     1200

aaccuggcga auaggaagua uaacgaugag uugauagagg aacuccugca cuuaaguuuu     1260

accaaauucg gacaucuguc ucuaaaggcc cuccgcaaua uucugccuuu uauggagcaa     1320

ggcaucccau accaccaggc augugaaaaa gcugguuauc aguucaacaa gaaggucaau     1380

cgagagaaac agaaacuuuu gcccccuguu ccagccaaag acaucgugaa cccgguagug     1440

auucgguccc ugagccaagu ccguaaggug aucaauggga uuauaaaaag auacggaucg     1500

cccucuucca ucuacauuga acucgcuagg gagaugggcc gcggcuauaa ggagcggaaa     1560

gaccuggaaa agcaguacaa caagaauaga cagaugaacg agggggccaa aaagcacauc     1620

cuugaacuga ggccugaguu uggagaucca cgaggucaug acaucuugaa auauaagcuc     1680

uggcaggaac aagauugccg cugugcauac ucauucaaaa guauucccau aaagcagcug     1740

uuugagaucg gcuaugccga gguugaccac auuaucccuu acagccgguc cuucgaugac     1800

ucuaauaaca auaagguguu aguccuggcu gcggaaaacc agaacaaaca gaauagaaca     1860

ccauaugagu gguuugggca ugaugaaacc aaguggcaag aguucacgag ccuagugacu     1920

acaaugaaag uaucaaagaa aaagaagagu cuccugcuua aaaagaacuu ugacgaagaa     1980

caggcagagg aguucagguc ccgucacuug aaugauaccc gcuacauuac ucgguacauc     2040

aaaacauuca uagaggacaa ccugcaguuu agaaucgaag agggaaagaa aaccuauguu     2100

cacacgguga auggcgccua caccagccuc augaggaagc gaugggguuu caacaagaau     2160

cggcaagaga acgcucugca ucacgccuua gaugcaguca uuguggccgu cucucugccc     2220

uuuaaacaua aggugcgcgc uuauuucaaa agaagggaag aguacgccca caaucagcuu     2280

aagcgggaag gggaguuuuu cccggaaccu uggcccggcu uuauccauga guuggagauu     2340

cgaaugaugc aggacucaga aaacaucaaa cucgcaaugg agagucugaa ucuaaagaca     2400

uaugaugaga gcuucauaaa agaacugaag ccaauuuuug uuuccagaau gccuaagcgc     2460

gcgaugaaag gacagaucca cgaagagacu cucaagaggg ugcguggccg gaccgacgac     2520

ggguacauua gaacaguaaa gaaggucaaa cuggaucaaa uccccuucga caagaacgga     2580

gauuuuccaa uguucgguaa ggagacugac augaaaaccu augaagcuau aaagucuagg     2640

uaccuugagu cgggcaaaga uaagaaaaag gccuuuaaua cacccuugua caagccugcc     2700

aaaaacccag aaaaugcacc gaucauucgc agcaucaaga uugaggaaaa agcuaaccga     2760

gugguuaugc uggacgagaa gaccgccgca gauaacgcuu ccaucgugag aacugagguc     2820

uuccggcaca aagaaacggg gaauuauuuc uuaacacccg uguacguagc cgacgugcuc     2880

gcgaagaagg uuccugauag gcugauaacc acuaaaaagc cauauucaga guggcgcaaa     2940

auuaccgacg aauacgaguu ucuuuucagu cuguauccca acgauuugau ccgugucaag     3000

augccuaaag agaaggaaac aaagacccau acuggagaga aacacgugug gcaggaaaau     3060

uuuauguacu ucaagggcgu gcauucuggu agcgccgggg ucagaaugau cgaccacaug     3120

aacuccuuug aggaugaaau uggcguucgg aaacucgugg uguucgagaa guaucaggua     3180

gacccacugg gaaggaugaa uaaaguccac ggcgagaagc gccaugggau cuga           3234


<210>  2692
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2692
augaaguaca gcaucggccu ggacauuggg auagccuccg ugggaugggc ugucaucaac       60

gaggauauug aaagaaucga ggaccucggu guuaggauuu ucgauguggc agaacagccc      120

aaaaccggcg ccucucuuuc aacaccucgg cgccuggcgc gaaguacucg uaagagauug      180

aggcggcgca gacacagggu agagcgggug cgcaaauuua ugauccaaaa gggguuacug      240

acgcaggucc agauggaaca gcuauaugac uggaaagaug gagaccucga uauauggcug      300

gugcgaguua augcucuuga gagaaagcug agcgacaggg aguucgcccg gaucuuggug      360

cauuuugcaa agaaccgugg cuuccaaucc aaucgcaaau cugaacucca ggaggaagau      420

aacggugccg uccugucggc ugugcaggag aauaagagac ugauggagga aaaaggguac      480

cagaccauuu cagagaugcu cuuauaugac gaaaaguuug agggaacaaa aaggaacaag      540

ggcggcgaau acacucacgu aguugcacgg agcgaucuga agcgagagau ccaacuuauu      600

uucgagaaac agcgcgccua ugggcaugcu uuugccacca uggaaaauga ggacaaguuc      660

uugcagaucu ggaguuccca gagaccauuu gcgacaaaag augacauagc aaagaaaauu      720

ggaaacugca ccuucgaacc gaaggagaaa agggccccca agucuacuua cacauuugaa      780

cgguuccgcg cucuggacaa gcuaaauaga cucaggaucc ugagcgguac cuccccugcc      840

cguucacuua cgcaagagga gcgggaucug auuuugaaca gucucuuuag caaaaaggaa      900

gugaaauaua aggagcugcg aaaauuacug aagcucaaug acgaacagag auucaacgag      960

aucuucuacg auccaaauga aaagaucgag aaaaacgaga auaggacuuu ucuuucucug     1020

gaaucccagu acaagauucg caaaauauug caaaagaucg agggcaaauc acuaaacgac     1080

accuaucggc ccgucgaucu ggacacaauu gcauacgcuc ucacuguguu caaggaugac     1140

caggauauca gagacuaucu gcagaaccag uacguggcca gcaaugggaa gaaagaacuu     1200

aaccuggcga auaggaagua uaacgaugag uugauagagg aacuccugca cuuaaguuuu     1260

accaaauucg gacaucuguc ucuaaaggcc cuccgcaaua uucugccuuu uauggagcaa     1320

ggcaucccau accaccaggc augugaaaaa gcugguuauc aguucaacaa gaaggucaau     1380

cgagagaaac agaaacuuuu gcccccuguu ccagccaaag acaucgugaa cccgguagug     1440

auucgguccc ugagccaagu ccguaaggug aucaauggga uuauaaaaag auacggaucg     1500

cccucuucca ucuacauuga acucgcuagg gagaugggcc gcggcuauaa ggagcggaaa     1560

gaccuggaaa agcaguacaa caagaauaga cagaugaacg agggggccaa aaagcacauc     1620

cuugaacuga ggccugaguu uggagaucca cgaggucaug acaucuugaa auauaagcuc     1680

uggcaggaac aagauugccg cugugcauac ucauucaaaa guauucccau aaagcagcug     1740

uuugagaucg gcuaugccga gguugaccac auuaucccuu acagccgguc cuucgaugac     1800

ucuaauaaca auaagguguu aguccuggcu gcggaaaacc agaacaaaca gaauagaaca     1860

ccauaugagu gguuugggca ugaugaaacc aaguggcaag aguucacgag ccuagugacu     1920

acaaugaaag uaucaaagaa aaagaagagu cuccugcuua aaaagaacuu ugacgaagaa     1980

caggcagagg aguucagguc ccgucacuug aaugauaccc gcuacauuac ucgguacauc     2040

aaaacauuca uagaggacaa ccugcaguuu agaauggaag agggaaagaa acaauauguu     2100

cacaccguga auggcgccua cacgagccuc augaggaagc gaugggguuu caacaagaau     2160

cggcaggaga acgaucugca ucacgcuuua gacgccguca ucguggcagu cucucugccc     2220

uuuaaacaua aggugucagc cuauuucaaa cgcagagaag aguacgcuca caaucagcuu     2280

aagagggaag gggaguuuuu cccggaaccu uggcccggcu uuauucauga guuggagauc     2340

cggaugaugc aggauaguga aaacauuaaa cucgccaugg agagccugaa ucuaaagacc     2400

uaugacgaau ccuucaucaa agagcugaag ccaauauuug uuucucgaau gccuaagaga     2460

gcaaugcgcg gacaaauuca cgaagagaca cucaaaaggg ugcguggccg gacugacgau     2520

ggguacauca gaaccguaaa gaaagucaag cuggaccaga uucccuucga uaaaaacgga     2580

gacuuuccaa uguucgguaa ggagacagau augaagacuu augaagcgau caaaucgagg     2640

uaccuugaga gcggcaagga caaaaagaag gcuuuuaaua cccccuugua caagccugcc     2700

aagaacccag aaaaugcacc gauaauccgc uccauuaaaa ucgaggaaaa ggccaaccga     2760

gugguuaugc uggaugagaa aacagcugcc gacaacgcau caauugugag aaccgagguc     2820

uuccggcaca aggaaacugg gaauuauuuc uuaacgcccg uguacguagc ugaugugcuc     2880

gccaaaaagg uuccugacag gcugaucaca accaagaaac cauauaguga guggcgcaag     2940

auaacugaug aauacgaguu ucuuuucucu cuguauccca acgacuugau ucgugucaaa     3000

augccuaagg agaaaggaac caagacacau accggcgaaa agcacgugug gcaggagaau     3060

uuuauguacu ucaaaggugu gcauagcggg uccgcgggcg ucagaaugau cgaucacaug     3120

aacucauuug aagacgagau cggaguucgg aagcucgugg uguucgaaaa auaucaggua     3180

gacccacugg gcaggaugaa uaagguccac ggggagaaac gccauggaau uuga           3234


<210>  2693
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2693
augcagcaaa agccccagag auacauccug ggccucgacc uugggauugc cagcguggga       60

ugggcuugcg ucugggcaga ugagaacgaa cacccuaccg gucuguugga cuguggcguu      120

aggacauucg agcgggccga agugccaaaa acuggggauu ccuuagcgcu ggcucgccga      180

caggcccguu cuguaagaag gcuaauacgg cgcagagcac auaggcuccu gcggcuucgc      240

cgacuguuga agagagaggg agugcuccug ucagccgacu uuaaugaaaa cggccugguc      300

aggggucucc cgaucgaugc uugggcauua cggguggccg ggcuggaccg uaaacuuagu      360

aauaaggagu gggcugccgu uuugcugcac cuagugaaac aucgcggaua ucucagccag      420

agaaagaacg agacgcaauc caaggauaaa gaacugggca ggcuucuggc gggcgucaau      480

gggaaccggg aggcauugca gaaucgaucu gacgaauacc gcaccgccgc ugagcucgcc      540

gugaagaaau ucgcagcuga gaacaaugga ucgcugagaa acaagggugg cgauuauuca      600

cacacauuug acaggaaaga uuuacagcgg gaacugcauc uccuuuucga gcgccagaga      660

gcccuggaca aucccuuuac uagcagugaa auggaggcgg ccguagauga auugcuaaug      720

aagcaaaggu ccgcacugca gggggaggcu guucucaaga ugcugggacg uugcaccuuc      780

gagccuucug aauacaaagc cgcuaagaac acauauagcg ccgagcgguu uguguggcuu      840

accaaacuga auaacuugag gauucaguac aauggcgaag agagagcacu cuccgaagcc      900

gagaggcgcc uguuacugga cgagccauau cagaaggcua aacuaacuua caagcaaguc      960

cggacaaucc ucgaacugcc cgagucagcg guguucaaag gucuuagaua cgggcacgga     1020

gaagacggcu ugaaggcaga gaguggcacc cugauggaaa ugaaggccua ucacgcugug     1080

aggaaagccc ucaaggaugc agggcuggag ggagaguggg aaaaccgcau ucgaaacccu     1140

gagcuucugg acgccaucgg uacggcuuuu agcuuguaca aaacugauga agacauacgg     1200

gccaaucucg cagcguuccg ucugucugag aacgucuuag aggcucugcu agccggccuc     1260

aauuuugaua aguucguuca ucugucccuu aaagcauugg ccaagauccu gccacucaug     1320

gaacagggga ugagauauga cgaggcuugu aaggaagccu acggagauca cuauggcgca     1380

aaagagcagg aagaccauag gcuguuaccc ccuuuugugg cugaugacau ucgcaaccca     1440

guaguguucc ggacccugac acaagccaga aagaucguca augagauugu gaggcgauac     1500

gguucaccgc uucgcguuca caucgagacu gggcgggaag ugggcaagag cuucagugau     1560

agaaaagaga uagaaaaacg acaggaggaa aaccguaagg agcgcgagcg ggcggccgcu     1620

cacuuuaagg aauuguuccc caauuuugca ggagagccua aagcccagga cauucucaag     1680

cugagacuau augaacagca acauggcaaa ugccuguacu cugggaagga gaucgaccuc     1740

aggcgacugc cagaaaaagg auacgucgag auugaucacg cccuucccuu cucccggacc     1800

ugggacgaua gcuuuaacaa uaagguguug guacugggua aggagaacca ggacaaacgc     1860

aauagaacac cuuaugaaua cuuagauggc aaggggaacu cgggagcuug gagggcauuc     1920

gcggagcggg uuucuggcug ucgauuucca uaugccaaaa agcagagacu ccugacccag     1980

aaacuugacg aaaagacgga gcaagguuuc cuggagcgca auuugaacga uacuagguac     2040

guggcucguu uucucugcca guucaucgaa gagaaaaugc aucuggaagg ggccggcaag     2100

aagcgggucu uugcauccaa uggacagauc acagcucuac ugagaggcuu cugggggcuc     2160

aaaaagguga gggaggaaaa cgaccgccac caugcccugg augcagucgu gguugccugu     2220

ucaaguguga gcaugcagca gaaaauuacc cgacacuuua gauaucggga ggagcuugaa     2280

cacauagaca gggagacugg agaaguaauc caugguuucc cccaaccgug ggaguucuuu     2340

cgccaggaag ucaugacacg uguguucucc gaugacccug uuucugaguu ggcugagaag     2400

cuggauagca gacccgaagc ggugcacgag uuugucaccc cauuauucgu gucacgggcc     2460

ccucauagga aaguaacggg ccaggggcac cucgaaacca uucgcagugc aaagcgacug     2520

gacgagaaga ucuccguggu uaaacuuccc cugacacaac uaaagacuaa agacauagaa     2580

agaauugucg gauacccaaa ccgggagccc gcucucuaug augcccuggc cgcuagguug     2640

gcagccuuua aggacgaucc ugcgaaggcu uucagcgagc cauuuuacaa aaccggcaag     2700

gacgguaaau gcggggugcu ggugaaggcc guccgcguug aaaaugugca gaaaucuggc     2760

uuacucguga gaaaggauga gucaggaaaa augcagggcg uagcagacaa cgccacaaug     2820

gucagggugg auguuuucgg gaagaaugga aagaacuacc uggugccggu cuaugcuugg     2880

caaguggaaa aaggcgagcu ucccaaucgg gcaguugugg ccuacgcgga cgaggaagau     2940

ugggacgaga uggaugacag uuuugaguuc aaguuuagcc uguauccuaa cgauuuggua     3000

gaagucguga cuaaaaagga caaauucuuu gguuacuauu ccggguucaa ucgugcuacc     3060

ggagccauca acaucaagga acacgaccuc ucuaaaucga aggugaagaa uggcgucuac     3120

gaggguauug gcguuaaaac agcacagagc uuccagaagu aucaggugga uacccugggg     3180

aaaacuaucc gccuauguaa gccagagaaa cgaaugggau uuaagaagaa auga           3234


<210>  2694
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2694
augagauacg ugcugggccu cgacaucggg auugccagcu gcggaugggc ugucauaaac       60

caggagaagg aaaggaucga ggaucuuggu guucggauuu ucgacaaagc agaaaauccc      120

aaggauggca aaucccuggc cgcgccucgc cgagacgcuc guucuaccag aaggacauug      180

cggcgcaaga aacacagaau gcaaaggauc aagguguuac ugguaaagca ugggcuacuc      240

ucaaaaacug agcuggauca ccuuuaugaa agugccacgg agguggacgu cuggaaccug      300

cgguuggaug cacucgagcg caagcuguac ccaaaagaau uugcccgaau ccugauucau      360

cucgcuaaga gaaggggauu caaaagcaau cggaaggaga ccacauuauc cgaaaacggc      420

cagauacugg agucuaucuc ggagaaucag cagauuaugg aaaagcaaaa cuaucguacu      480

gugggugaga ugauccuuaa agacaagaaa uuugaaaauc acaagcgcaa caaaaauggg      540

accuacguug gaacagugac cagacagcag uugcaggaug agauucaaau gaucuuccau      600

gcacagaggc uguauaagaa cgacuacgcc acugaagagu uugagucaag cuaucuagaa      660

auaugggcua gucagcggcc guacgccucc aaagaucaga uugagaagau gaucggcuac      720

uguacacucg aacccaagga gaaacgagcg ccuaaggcau cuuggagcuu ccaauauuuu      780

gucgcccugc agaccauuaa uaaccuucgc cugaucucca aagacagaau cgaagaguug      840

ucauucgagg aaaagaaaca gauuaugaau cucgcucugg agaagaguau agugaaguac      900

aucgauauua ggaaauuacu gagcguacca aacgaauuuc aauucaauuc ucucuccuau      960

ucagccgaca cgguugacac ugcaguggag agccggaagu gcaucgaacu uaaagaguac     1020

cacaguauaa acaaggucua uaaacagauu uacggcgaga acgcucugaa uuugcuaccc     1080

aaguauuacg auaccaucgc cuacgggcug acaaucuuua aggacgauaa agacauucuc     1140

gaauaucugc agaacaagua cgugaaugcg aaaggaaagc cuaucucuaa cuucgccaau     1200

aaaacuuaug augacaccuu uauucaggca cuucugaacu ugaauuucac aaagaugggu     1260

caccucuccu ucaaggcucu gaaaaacaua aucccauuuu uagaggaagg ccugagcuac     1320

gauaaggccu gugagaaagc uggguauaau uucaagggaa ccucguacac ggagcaaacu     1380

aaauaucuac ccgugauucc ucagaacaca aauccagucg uucaucgcgc ccucucucag     1440

accagaaagg ugaucaacgc aaucauuaaa aaguacggcu ccccgaaugc cauacacauc     1500

gaaacugcua gggagcuguc aaagacauuu caggaacgua aagagauuga caguauguac     1560

caagauaaca gcaagaaaaa ugaacaugcg auccacaaac uuaaggaguu ggguaacauu     1620

aaccccuccg ggaucaauau aguaaaguuc aagcugugga acgagcagga cggacggugc     1680

auguauucug gcaaauacau cgaaccucac cgacucuuug aggaaggcua uaccgaggug     1740

gaucauauuc ugccauacag cagaucacuu gacgacaguu auaauaacaa gacgcugacc     1800

uuggggacag aaaaucagag gaaaggaaac aagauccccu acgaguauau ggguaauacu     1860

uccauuugga acgauuucga gacccgcguc cagagcaaua aaaagaucaa caagaaaaag     1920

caacagaaac uccuguuaca gcacuuuucu uacgcacggg aacaggaguu cauagaaaga     1980

aaucugaacg acacaaggua cgccacuauu uaucuaucaa gucucaucca acagcaucug     2040

auuuuuagcg aguccucucg caagaaaaag gugcacaccg uuucgggcau cauaacaagc     2100

caucuucgau cccggugggg guucaauaag gaucguaaag aaggacacau ccaccaugcu     2160

uuggacgccg ugauugucgc agugaccuca gaucacauga uccagagagu aacuaaguac     2220

uauaaacuga aggagcucaa caggaaucug caagccaaac gcaugccuuu uccagagccc     2280

ugggaaggcu uccgguuaga gcuggaagcu agaauuaguc cgaacacgca gcaguaccuu     2340

aaggguuuga gguuuaaaaa cuaugccgac guuaaucucu cugagaucaa gagcauauuc     2400

gugucccgaa ugccuaagca uucaauuaca ggggagcugc accaggaaac cauccgcaaa     2460

uucaucggcu acaacgagaa gggaaaaguc cuaacugcaa uuaagaccaa acuggaagau     2520

auccccuuug acgcgaaugg cgauuuccca auguauggga aggagagcga ccucuacaca     2580

uacaacgcua uuaaggaacg guaucugucu cacaaaaagg auaaaagaaa guccuuucaa     2640

gagccucuuu acaaacccac caagagugga gguauaggcc cauugaucaa gagcauuaaa     2700

aucauggaca cuaggacaau ugugaauccc gucaaccagg ggaagggagu gguuuauaau     2760

ucuaaaaucg cccguacgga uguguucaag aaagaggaaa aguacuaucu gauaccuauc     2820

uacaccauug acuuaaugaa gaacauccuc ccacagaaag caauuacugc cggcaagggu     2880

uaugaggacu ggacaaccau cgauccgucc uuuacauucc uguuuucacu uuuccccaau     2940

gaccugauac agauuguacc uaguaaaaac aagacuauca aagcucgcac cacggucagc     3000

aagaaggaag uguugcuccc aucgcugacc ggguacuuua aaggcguuca uuccggaaca     3060

gccggcauca cuguggagac ccacgauggg ucugucauug caaauguggg aagcaagcaa     3120

cuacugcuca uagaaaaaua ccagguagac gugcugggua acuauacaaa gaucaaagag     3180

gagaagcgga uuggccuuac uaccuacaau gauaacaaaa agaucgcugc cuguuga        3237


<210>  2695
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2695
augaagcagc aaccccagag auacauccug ggccuggacc uugggauugc cagcguggga       60

ugggcucuga uauuggucga ugaggaaggu aacccuaucg gcuuacugga cugcgggguu      120

aggaccuucg agcgggcaga agugccaaaa acaggagauu cccuagcccu cgcgcgccga      180

caggcucguu cuguaagaag gcugauucgg cgcagagccc acaggcuucu gcgguugcgc      240

cgacuccuga agagagaggg cgugcuguca gcagccgacu uuaauaguga uggucucccg      300

aacuuaccca ucgacacuug gcagcugagg gucgcugggc uugaacggaa auugacgaau      360

caagaguggg cagccgugcu gcuacaucuc guuaagcacc guggauaucu gagccagcgc      420

aaauccgaga cccagacaga aaacaaggag cuuggcuguc uguuggcuuc uguggccaaa      480

aauucgaaac uccugucaga aaacgcgaau caguacagaa cuccugcaga guuagccguc      540

aggcuguucg cugagcaaag cggccaucuc cggaacaaag ggggagauua uacccacaca      600

uuuaaucgac uugaccugca gaaggaauug caucuacugu ucgagcagca gcgcaccuuu      660

gguaacccau ucgccagugc agaacuccaa uccaaggugg auggccugcu uaugaugcag      720

agaucugcuc ugcaggggga ggccguauug aaaaugcucg gaaagugcac uuuugaaccc      780

agcgaguaca aggcggccaa acacacauau uccgcagaga gguucauuug gcugaccaag      840

uuaaauaacc ugcggauccu agaaaauggc acggagcgcg cucucaacga agccgagaga      900

aaacugcuuu uggacgaacc uuacaagaaa gcuaagcuga cuuauucaca agccaggaaa      960

cuccuggaua ugagucacga cgcaaccuuu aagggucuuc guuacgggaa ggauagcgag     1020

ggacugaaug ccgagaacgg cgcuuugaug gaaaugaaag cguaccauaa gauacggaca     1080

guucucgaca aagcaggccu gaagggggag ugggaaaacc gaaucagaaa uccagagauu     1140

uuagacaaaa ucggaacugc cuucucucug uauaagaccg augaagacau ucaggcugcc     1200

cuagcagauc ucaggcugcc cgagaacgug cuuaaugccu ugcuggcugg ucucaacuuu     1260

gacaaguuca uccagcuguc cuuaaaagcc cugucaaaua uaaugccucu uauggagcgc     1320

ggcauucggu acgaugaagc auguaaggag gucuaugggg accacagagc guuggcugaa     1380

agcaaaaacc agaugcuccu gccaccgauc cccgccaaug agauuaggaa cccuguggug     1440

cuacgcacac ugacccaaac gcgaaagguc aucaaugcaa ucauucggcg uuacggaagu     1500

ccacagagag uucauauaga gacugccagg gaacucggca aaucuuuuaa ggaucgcaag     1560

gagaucgaaa aacggcagga ggaaaacaga aaggagaggg agcgagcugc cgaacacuuc     1620

aaagagcugu ucccccacuu ugcuggugaa ccuaaagggg gcgacauucu uaaguugcgc     1680

cuguaugagc agcaacaggg aaagugcuua uacuccagca ucccaauaga ucucggccgg     1740

cugaaugaaa agggguaugu ggagauugac caugcacuuc ccuucucgag aacaugggau     1800

gacucuuuua acaauaaagu acugguguug ggauccgaga accagaagaa aaggaaucag     1860

accccguacg aauaccucga cggugccuca aacagucaac guuggcgcga guuccaggcg     1920

cggcugcagg gcuguagauu uagcuaugcc aagaaacaga ggauccaaac ugcuaagcua     1980

gauuccaaaa cagaacaggg guuccuggag cgaaaccuca augacacccg guacaucgca     2040

cgcuuucugu gcggauucau ugaagagaac cuucacuugc agggcgaggg uaagagaagg     2100

gucuuugccu cuaaugggca aaucacggcu cuguuacggg gccucugggg acugaagaaa     2160

gugcgagaag agaacgauag acaucacgcc cuugacgcaa uuguuguggc guguagcacc     2220

gucucaaugc agcagaagau aacaaaagcc augcagcgca gggaaacucu ggagaccgug     2280

gauacagaga cuggcgaagu aaagacccgu aucccucaac ccugggacuu cuuucggcag     2340

gagguuauga ugagaguguu cagugauaca ccacaugagg aacuagcuga aaaacucucc     2400

gggaggccug aggcccugca cgacuuuguc agccccuugu ucgugucucg cgcaccaaau     2460

cgaaagauga ccggacaggg ucaccuggag acugucaagu cagcuaaaag auuaagugaa     2520

aacauuagcg ugguucggaa gccccucacg gcccugaaac uuaaggauau cgagaauauc     2580

gugggcuauc cugacaggga accagcacug uaccaggccu ugcgcgagcg ucuccaggcu     2640

uauaaagaug acccgguaaa ggcguucgcc gaacccuuuu acaagccugc aaagaacggg     2700

ggacagggcu cccuggucaa ggcugugaga guuaaagaug ugcagaagac aggugucaug     2760

gugcggaaag accgcggggg cauugccagg ggaauagccg acaaugcuac caugguacga     2820

guggauguuu ucggcaagaa ggggaaaaac uaucuagucc caguguacgc auggcaagug     2880

gccaagggaa uccugcccaa uagagcgguc guugcuuacg ccgacgaggc agauugggac     2940

gagauggaug acucuuauga auuuaaauuc acucuuuacc cuaacgaucu cguggagauu     3000

aucucgaaga aaggccggau uuuugguuau uacagcgggc ugaauagggc caccggagcu     3060

aucgacauaa aggaacauga uuuguccaaa gagaaggcaa agaacggcau ccaccaauca     3120

guggguguaa aaacaguccu gaguuuccag aaguaucagg uggacccauu aggcaaagaa     3180

auuugccucu guaagguuga gaaacauccc gagcucaauu cuaagaagaa aaaguga        3237


<210>  2696
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2696
augggcuacc ugaucgggcu cgacauugga auaaccagcg uggguuaugc cguccuucag       60

acagauuuca agggcaaccc cuacaaaauc cuguugauga auacuguuau uuuuccugug      120

gcugaggcac caaagacggg ggaauccuua gccgcgccgg uaagacagaa aaggucuuca      180

cggcgcacca accgacguac aaaguucaga aaacugaggg ugcaaaagcu auuuguccgg      240

uucggacucc uggagagugc ugacguggcc acuuuucuua aucagaaggu uccccaccag      300

gauaucuggc aacugcgcaa agacgcauug aacagaaagc ucagcaauag ggaacuguuc      360

gagauucugu auuucuucgu gggccaucgg gguuuuaaau ccaaccgccg aucugaaguc      420

accgggaagc agccuaaaga uaaucaggug guacucacau cgauccacga cagaggaaac      480

accuuaaaug auuucgacuc acugggcgag augaugcagg agcuuccaga auuuaagacu      540

auaaagcaua acaaagccua caggcccgau aauacaaucu auccuuugcg ggcuuggauu      600

gagcacgaac ugagcgcaau cuucaguguu caacagaaga acaaucguca gauuaccgac      660

aacuuuauca cgcaguaccu agauauauuc cgcucccaaa gagacuucga ucagggccca      720

gccgcucccu cuagguuugg gggaaauaug auugagcgga uggugggucc ugacagccuc      780

gacgcccaug agaaacgagc ggcaaagcug acuaacaccu ucuuucgcuu caacuuucuu      840

uccacacuga auaacuugaa aauccagcuc acucugggcg ccgaauuuca cccauuaacc      900

acaccgcaaa gagagauugu ccugcaggcu gccacccuca agucacuuaa uuauaaacag      960

aucaggcaca agcugcaguu gcccgauagu gcacgguuua accuagugcc uuaccaaagc     1020

aaugaagacc caaucaagac ggagucuaaa cauuaccugu ucaagcgcaa agcucucacu     1080

cagauugaag ccgugacaga uaacccccag cugauagaca agaucgggac cauucuugcg     1140

uauuuuaaag gagauaagac uagacaggag aagcuguccg ccuugaaucu cucaagggca     1200

gagaucaaac aacuguuacc uauaaaccag ccaggcuucg guaaucugag ccuaaagaca     1260

augcacaaaa uucuccccua ccuggaacag gggcaucuuu aucacgaggc ugcccaguug     1320

gcuggauacg acuuucaaaa caaucugguc gaucgucagu aucuccggga caaccugaag     1380

aauccgguug ugaaccgagc caucggcaaa accaucaagg uagugaaugc aauuaucaag     1440

aaauacggcg auccugacgc cauucacaua gaacuuacga gagagaucaa gcauagucac     1500

gaagagaggc agaaaauugc ucgccagaug aacaccaaca uggaucaaaa ugacaagguc     1560

gcggagcauc ugcgggaaaa cgggaucccc gugaauggac agaacaucau uaaaucuaag     1620

uuguucuaug agcagaaggg uauagaccuc uacuccggcc aacagaucaa ugaacaggau     1680

cuguacacag aggaccacua ucagguugau cauauuaucc cauacagcau uucguuuaac     1740

gacucuuuca cuaauaaaac cgugacaaga acuuccaaca aucaaaacaa ggcaaauagg     1800

uuaccucugg aauaucuauc agaggagaaa caggaugccu uccgcgcucg agucaugagu     1860

aacaucagca auuaccggaa gcgucagaac cuccugaaag aagggauaac cgaggacaau     1920

agacagcaau ggaagacaag gaacaucaac gauaccggau auauuaaucg ccuuuugucc     1980

caguaccugc ggaacaaugu gcaguuuacu aaaaagucuc acaaccccgu auucacgguu     2040

aauggcgcca caaccgcaua uaucagaaag agguacggua uuaucaaaaa ccgacaggcc     2100

acugaccucc accaugcugu ggaugccguc auaauugggu gcaucagcaa uaccuuugug     2160

ucacgccugg caaaguacag ugaagcgcaa gaggcucggu aucacaaaca guuauggcag     2220

auuacacaau acagacagga gcauaaggac cuguccacca ucgaauauca ggagcuuaua     2280

aaacaguucc cauugcccug gccugauuuu aggcacgaac ucauggcccg ucugagcacu     2340

gacccagcaa agaugauggc ccaccauaca ugggagcacu acacggauga agagaucucu     2400

caacuaaagc cggcuuucgu cauucgcuca gccaaccaua aagugaccgg cagugcacac     2460

gacgacacua ucuauagcaa gaaacuguac gaucagcggg gaguuggccu ccugagaaca     2520

ucccuucaga auuugaagua ugacucuaaa aaggauggga uuaagaacua cgagaggcug     2580

aaagacggau cgaauagcuu aguguacgau ucccuccugc gagcucuuaa ggaacaugac     2640

gguaacgcca aaaaggcguu ucccgauaau cagcuguuga ucucaaccgg caaccaccaa     2700

cagauaauua cacacguaaa aacugucaag aagaucaccc auccuaucga ggugaaucgg     2760

gacacggcca uugcuaucgg gggacgcaug auuagaguug auauauucaa acaggcaaag     2820

gcccagaaac ucaucggcau uccaguguau gucaacgaca ccgugaagcc cacacugccu     2880

aacagggcuu guaccagugg uaaacgguac ucugauuggu aucaacuaac ucgacaggac     2940

caguuucugu gcagccucgu auccaaugau cuggugcuug uugaaacagc cucaccauug     3000

aaggucacua acaauaucac caacgaggaa gugcagacga cacaacuguu auguuacuuc     3060

agcucuuuua auauugcaaa cgcgcagauc aauuuccacu ccauaaacaa ugacaguagc     3120

aucaacccca uugugcucaa uaaagucacc uaucugacua aguaccagcu ugacuaucug     3180

gggaacuacu uuccgguuaa gcaugagucu agaaaaacau ucaaucgcaa gagguga        3237


<210>  2697
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2697
augcacacca gacuggcccu cgacaucggc acaacuagca uuggguggau acuuuacgag       60

cuggugaacg gacagcccgc uuccaucuug gaugguggca cgaggauuuu cucugacggg      120

cgggaagcaa agaccggaca uucauuagcc gucggccgcc gaacagcgcg ugguaugaga      180

aggcggcgcg auagauaucu gaggcggcgc gcugcccuaa ugaaacgacu cgcagccauc      240

gggcugaugc cugagagucc agaagcugca gcccaacuug cuacucugga cccguacgag      300

uugagagcca ggggacucga ucacccccug ccuccagaac aucugggccg ggcgcucuuu      360

cacuuaaauc agcgucgcgg cuucaagagc aacagaagga ccgacaaagg gaauaacgag      420

ggagguauga uugcagaugc cuccgcucgg cuggagcagg ccaugauggc agcuggcgcc      480

cgaucuuaug gggaauuucu ucauaagcgc agagcggagg ccacagcacc cagggcuacc      540

ccugccguuc ggacucgcau caauccacac uugacaggag aagacggcaa agagcagccc      600

gguuacgauu ucuauccuga cagaaagcac cuggaggaug aauuucauag gcuaugggag      660

gcucaagccc gucacaaccc agaccucacc ccggcaaugg gggaucugcu uuuccagacg      720

auauuucauc agcggccccu gaaggccccu gcugugggac gaugcuuguu ccucgacgaa      780

ccaagacugc ccaaagcgca cccuuuaacu cagcaaagga cccuguacga gacaguaaau      840

augcuccgca ucgcaccacc cggccagccg aagcggagac uuucgagggu gcagcgcgac      900

cagauugucc acgcccugaa caaucgaaaa aucuuggcuu caccuggcaa gaacgugcua      960

acucuggccg caauugggaa acuccugaag cugcggcccg gagaagccuu uagccuggau     1020

accgcuaauc gugacgccau cgcaugugau ccaguuagaa ugaguuuggc guaugagggu     1080

agguucggcc cucaaugguc cgcucucucu guggagagcc agugggaacu gauacagcgc     1140

uuacgggacg aucaagacgg ggccgcacug gucgauugga uuauggacag auacggauuu     1200

gauagggacc gagcccgcgc uaucgccgau gcaccccuac cagagggcuu cggucggcuc     1260

ggggaaacag cuaccagagc cgugcuggcg guacuuauug gcggaacgac ugagcaggaa     1320

guuuugacau acuccgaggc cguggcugca ugcggcuggg accauucaag gcuggggacc     1380

ggagaggucc ucgaccgucu gcccuauuac ggucaggugc uugaucgcca cgugcugccu     1440

ggcacugggc ggccagaaga cgaugagauc acaagauuug gaaggaucac caacccgacg     1500

guccauauug gcuuggguca gguuaagcga cucgugaaua aaguaauaga cgccuggggg     1560

cggccccacg aaaugguggu cgagcuggcu cgcgauuuaa agaugaguga agaccaaaaa     1620

cgcaaccugg ccaaggugaa ugcacagaac cagagggcgg ccgagaugcg gagcgcuaaa     1680

cuaaucggcg aucucaagau ugaggacaac ggacgaaaua gaggcauccu gcgcguuugg     1740

gaagagucug ccgcagaugu gcuucauagg uguugcccuu auaccggggc ucagauaucc     1800

gccucaaugu uguucgacgg aagcugugau auugaccaca uccugccaua cagucguaca     1860

cucgaugacg guuucaacaa ucgggucauc ugccugaaau cugcaaacag agaaaagagg     1920

aauuuaacuc ccugggaggc cuggggcaac accccuaagu gggcugcgau ugaggccaau     1980

cugaaaaacc uuccacccgc aaaggcuugg cgcuuugccc cggacgccau guugcgauau     2040

gaaggggaga gagauuucac agcucgggca cucguggaca cucaauaccu guccaggcua     2100

gcccgcgaau aucuggagug ucucuacgcg gaagauaucg acccuggacc acacagcggc     2160

aaaaagcccc guagaccuau uuggguaguu accgguaaac ugacagagcu uuugcggagg     2220

cauugggggc uuaauucguu acuccccgau gcucaccgcg gcaccgccaa ggcaaagaac     2280

cgacuggacc auagacacca caugauagau gccgcugugg ucggagcaac ugaccaugcc     2340

acggugcagc ggaucgcgaa uauggcuaaa ggcaacgucg agaaugggcu ugaagaucug     2400

gccggaacaa uuuugccacc uuggcccggc cucagggacg aucugaaggc acgccuagag     2460

agaaccaucg ugucucacag ggguaaccau ggggccaucg acccagcugc ccggaaacag     2520

ggacgugaua cuaccuccgg ccagcugcac aaugacacag cauacggucu caccggcgaa     2580

acucugaagg ggguuccgcu ugugguaaac cgcaaaccua uugacauguu gacacccgcu     2640

augauagaua agauccgaga cccacaucug ucaagauggu uaacggccgu caccaaggga     2700

aaagagggca aggauuuuaa agcggccgug gaagcauuca gggcucaccc cgggccuuau     2760

cggggaauua gaagaguuag gcucaucgag ccaguggagg ucauucggau caaggaccga     2820

aacggcagac ccuacaaagg uuauaagggg gauaguaauc aaugcuacga aguguggugu     2880

cugccugacc gcacucuuac acaccaugua gugagcaccu uugccgcuca ccagggacag     2940

acaggcaagc cgcauccagc agccaaaagg cuggcccggc uauauaaggg ugauaugguu     3000

cgucucgaca gauccaaauu cggcucuguc auagcuacug uggagaaguu ugauaggaac     3060

gggcagcugg aauuggugcc ccacaaugag ggaaacgcgg accaacgcua ccggaaagau     3120

aagagcgacu cagucuucau ccgacugaga gcagccaccu uuauugcuag uggcgccagg     3180

cgcguuaucg uggacgaaau ggggcggauu agagauccug gaccaggcaa acauuga        3237


<210>  2698
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2698
augaaguacg gcaucgggcu ggacugcgga auuaacagcg uggguuauug uauaauggag       60

cucaauuccg augacgaacc caaaagaauc gucaggcuua acucucggau uuucaccgag      120

gccgaaaauc cuaaggaugg cucaagucug gcugcaccac gccgagaggc ccguggguug      180

agaaggcgga uccgcagaca caaacauagg uuagaacgga uucgcgcgcu gcuaguucga      240

gagaacaucg ugacacaggc ugagcucgac aauauguuua gcggaacucu guccgauaua      300

uacgaacuua gaacggccgc acuggacgcc ccguugaaca auaucgaguu cgcuagggua      360

cucauuaacc uggcacaacg gcguggcuuu aagucuaauc gcaaaucgga aaagucaagc      420

agugauaagg agaccgguaa acugcucucc gccaucaaga aaaacaagga gucuauugaa      480

gcugggggau auagaacagu gggcgagaug uuauucaaag acgaaaggua cgccaagcac      540

aagcggaaua aaggcgaggc guaucugaac acugucagcc gagauaugau cgaagcagag      600

auacaugccg uguuugcuuc ccagcgcaau uucgggaugc ccuuugccaa cgagaagauu      660

gaaaaugagu acaccgacau cgcacuuuca cagagaccuu ucgauuuggg accaggugaa      720

ggccccgaga acugcaaaag uccuuaugcu gggaagcaga uuaaaaacau gaucggacaa      780

uguacauuuu ucccagaaga gaagagggcc guuaaagcga gcuacucuuu ucagcuguuc      840

ucccuacucc agggcaucaa uaacauuacc cuggugucaa gcgacgguaa uacuuuuccc      900

cuuagucagg agcaacggaa gacacugaag gauuacugcu ucaaaaccgc caacgucacg      960

uaugcaucua uacgcaaggu guugcagauc ccucaggaau acacuuucaa aaauauuacc     1020

uauacagacg acgggaucga gaaguccgag aaaaagacua aguuucagca ccucaaacca     1080

uaccaugaga ugaagaaagu acugggaagc gcuauacaaa ccuuaacacc ggaagaacug     1140

gaugagauug gcgaaaucuu caccuuuuau aaggacgaga cgaucauuau caaacagcuc     1200

gaagagacug agauugauaa gucgcuuuac gaccuguugc uacagcugcc cacauucgaa     1260

aaggccggcc acauaucuac caaggcuugu aaaaugauca uuccuuuucu cgaggaaggg     1320

aaaacuuaug auaaggccug cgagugugca ggacugaacu ucaaagccca cacaaauaug     1380

caaaagcaga ugcuucugcc agcuaagucc gacgaauuga acgauguugu gaaucccguc     1440

gugagaaggg cgaucucaca gaccaucaaa guggucaacg caauuauacg ugagaugggu     1500

acgaguccua ccuacaucaa uauugagcuc gcccgggaac ugagcaaguc caaaaaggag     1560

cgagacgaaa ucaacaaaaa guacaaauua aaucaggcug agaacgaaag acugaagaau     1620

gagauuaucg auaacuuuaa gauaaaacca aacggccaag acaucauuaa gcuaaaacuc     1680

uggcaugagc aggaugacgg gaucugcccc uauacaggag uuaggauuaa guacgaaaau     1740

cuguucgacg auggcuaugc cgagaucgac cacauaauuc cguacucucu uagcuuugau     1800

gacucauuca acaauaaaau cuugacuacc gcaacacaga accgccagaa ggguaaucgg     1860

auuccucugg aauaucuccc cgagagugaa aaggaugggu uuagagugug gguaaacucc     1920

aauaucagga accaucgcaa acgacaaaau cugcuuaagg agcacuucac ugaggccgac     1980

aaaaaggaau uuaaaagccg gaaccugaau gauacccguu acuugaacaa gcuccuguua     2040

aauuauauaa acgacaaucu gcuauucaca gauuucuaca acggcaagaa aaagcacgug     2100

accucuguca acggagcugu gacugccuac aucagaaaaa gguggggcau uaauaagaac     2160

cgcgaggacg gggaucucca ucacgcaguu gacgcggcug ugaucgccug ugucacgcag     2220

ggaauuauca aucggauaac agcauauuca gccagaaacg aagcuccaua cgaucugaau     2280

accgagacug gugagauuaa agaaagguuu ccucuucccu auccaaacuu caagaaggag     2340

uuggacgccc gaaccacaau cgaagacgag aaacgccuga agcgggugcu caugaguuuu     2400

agcaauuacu ccuaugauga cguagaaaaa gcaaagcccu gcuucguuuc uagaaccccu     2460

cauaggaaag ugacuggcgc ugcccacgag gagacaaucc guucggggaa ggucgaagga     2520

uuuacgguga gcaaaaccga gcugacuaag uuaaagcuga caaacggcga aauugagggu     2580

uacuaugaac cagaguccga uacccuuuug uacaaugcgc uccugcagcg ccuaaacgag     2640

uucgggggcg acgccaaagc ugcauuugcc gaugaguucc auaagccgaa agcugacgga     2700

acacccggcc cugucgugaa gaaaguuaag acuaucaagc ggucaaguuc uaaugugaaa     2760

gccagaaaca agaaugggcu gggaguagca gauaacggua gcaugauuag gauagacguc     2820

uuuuacgugg aaaaugaagg cuauuacuuc guuccaaucu auguggcgga uaccgucaaa     2880

gagaugccca acgaagccug uccuuuuggg aaggacaaaa agaagauuau gacggaugac     2940

gauuuccucu uuucccugua cccaaacgac cuuaucaaaa uuaccgcuaa gaaagacaug     3000

aaguucucau uggccaauaa agagagcgga cugcccaaga acaaguaugc aaaugaggug     3060

uuaguauacu auucugcugc cgauaucucc aacgcaagua uaagcgugau caugcacgac     3120

aaugccuaca aauuccgagg cugcgguauu aagucucucg uuaaacugga aaaguacaca     3180

gucgauccgc uugggaacgu guccaaagug aaucgggaga agcgcaugua uuuuaaguga     3240


<210>  2699
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2699
augagacugg gccucgacau cgggaccagc uccauuggau gguggcuuua cgagacagau       60

ggugccggcu cugacgcuag gauaacuggg guggucgaug gaggcguucg gaucuucuca      120

gacggucgcg aucccaagag uggggcaagc cuggccgugg accgacgugc ggcuagagcc      180

augaggcggc gcagagauag guauuugcgg cgccgagcaa cguuaaugaa aguacuggcc      240

gaaaccggac uaaugccugc ugacccagca gaggccaagg cucucgaagc ccuggauccg      300

uuugcgcuua gagcagccgg ccuggacgag cccuugccuc ucccacaccu gggcagggcu      360

cuguuccauc ucaaccagcg gcguggguuu aaauccaauc gcaagacaga uagaggagac      420

aacgaaucug guaaaauuaa ggaugccacu gcaagguuag acauggagau gauggcuaau      480

ggcgcccgga ccuacgggga guuccugcac aagcgacgcc aaaaagcgac agaccccaga      540

caugugccuu cggucaggac ccggcuuuca aucgccaacc gcggaggccc agaugguaag      600

gaagaggcag gguaugacuu uuaccccgau agaaggcacu uggaagagga guuccauaaa      660

cugugggcug cccagggagc ucaccacccu gaacuaacug agacacuccg ugaccugcuu      720

uuugaaaaga uuuucuuuca gcggccacug aaagagccgg aagugggcuu gugccucuuc      780

agcggccauc acgggguucc cccuaaggau ccacgacugc ccaaggccca uccuuuaacc      840

cagagaaggg ugcuguauga gacggucaau caacuccgcg ugacugcaga cggacgggag      900

gccagaccac uuaccaggga ggaacgcgau cagguaaucc acgcucugga caacaaaaag      960

cccacaaaaa guuuguccuc uaugguucua aagcugccgg cgcucgcaaa agugcugaag     1020

cugcgagaug gugaacgguu uacucuggag accggcgucc gugacgccau agcuugugau     1080

ccuuugagag ccagccccgc acacccagac agguucgggc cucgcugguc cauccucgac     1140

gccgaugcuc agugggaagu gauuucacgg aucagaaggg ugcagaguga cgccgagcau     1200

gcagcgcugg ucgauugguu aacagaggcu cacggacugg accgagccca ugcagaagcc     1260

accgcucacg caccccuacc agauggcuac ggucgccucg ggcugacggc cacuacacgg     1320

auucuuuauc aauugaccgc ugacguugug acuuacgccg augcgguaaa agccugcggc     1380

uggcaucaca gcgacggaag aacaggcgag uguuuugaua ggcugcccua cuauggggaa     1440

gugcucgagc gucacgucau cccuggaucu uaccauccag acgaugacga uauaacccgc     1500

uucggucgga uuacgaaucc gaccgugcac aucggccuga accagcuuag aaggcugguu     1560

aaucgaauua ucgaaacaca ugggaagccc caccagaucg uggucgaguu ggcucgggac     1620

cucaagaaau ccgaggaaca gaagcgcgca gacauuaaaa gaauaaggga uacuaccgag     1680

gccgcuaaga aacggucaga aaagcuggag gaguuagaaa ucgaggacaa cggacgaaau     1740

agaaugcugc uacgccucug ggaagaucug aacccugacg augccaugag gcguuucugc     1800

ccauauacag gcacucggau uagcgcagcg augaucuuug acgguaguug ugauguggac     1860

cacauacuuc ccuacucuag aaccuuggau gacuccuucc cuaauaggac acugugccuc     1920

cgcgaggcca accgacaaaa gagaaaucag accccauggc aggcuugggg cgauacuccc     1980

cauuggcacg ccauugcagc uaaccugaaa aacuuaccgg aaaauaagcg guggagguuu     2040

gccccugacg caaugacgcg cuucgagggg gagaacggau uucuggaucg ugcccuuaaa     2100

gacacacaau auuuggcuag aaucagccgg ucguaccucg acacccuguu cacuaagggc     2160

gggcauguau ggguugugcc aggaagguuu accgaaaugc uacgccgaca cuggggucuu     2220

aauucucucc uguccgaugc gggcagaggg gccgucaaag caaagaaccg gacagaccau     2280

aggcaccacg cuaucgaugc cgccgugauu gcugcaaccg accccggacu uuugaaucgc     2340

aucucaagag ccgcgggcca gggugaggcu gccgggcaga gugcagaacu gauugccagg     2400

gauacuccuc ccccauggga gggcuuccgg gacgauuuac guguccgccu cgaccgaaua     2460

aucgugagcc auagagcuga ucacggaagg auugaccaug cagcccggaa gcagggccgc     2520

gauuccacag cggggcaacu gcaccaggaa acggcuuauu cuaucguuga cgacauccau     2580

guggccagca gaaccgaucu ucugucauug aaaccugcac agcuccugga cgagcccgga     2640

aggaguggcc agguacggga uccacaacua cgaaaggccc ugagagucgc uacugguggg     2700

aaaacaggaa aggacuuuga gaacgcccuc cgcuacuucg cauccaaacc gggcccuuac     2760

caggcuauua ggcgggugcg uauaaucaag ccccugcagg cccaggcgag aguuccagug     2820

cccgcccaag auccuauuaa ggcauaucag gguggcagca aucaccuuuu ugaaaucugg     2880

agguugccag acggggagau ugaagcucag gucaucaccu cuuucgaggc ccacacacug     2940

gaaggagaga aacgccccca uccugcugca aagcgguuac uccgagugca caaaggcgau     3000

augguagccc uggagagaga cgggaggcgc gugguuggac auguccagaa gauggauaua     3060

gccaacggcc uuuuuaucgu gccgcacaau gaagcuaacg cggacacucg gaauaacgau     3120

aaaucagacc cauucaagug gauucaaauc ggugcaagac ccgccauugc uagugggauc     3180

cguaggguga gcgucgauga gauaggacgg cugcgcgacg gcgguacccg accuauuuga     3240


<210>  2700
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2700
auggacgagg aagccaagau ccagcugagc aaagaguccu acgaagagga acucgagucu       60

cuucuggaga agucaggcga agaguucaga gauguguuuu ugcaagcuaa aaaggucuau      120

gacgcaauuu uacugaguga uauacuaagc accaaaaagc agaacuccaa ggccaaacuc      180

ucucugggga ugaucgaaag guacgacucg cacaagaaag aucuugagga gcugaagcag      240

uucguuaaag cgaauuugcc cgaaaagaca gcuauuuucu ucaaggacuc aagcaaaaac      300

ggauaugccg guuacaucga uggcaagacu acgcaggagg acuuuuauaa auuccucaag      360

aaagaacuga augggauugc aggaagugag cgguuuaugg aaaaggugga ucaagagaac      420

uuccugcuca aacagcgcac cacagccaau ggcguaaucc cucaucaggu gcacuuaacu      480

gagcugaagg cuauaaucga acgacagaag ccauacuauc cguuucuuga ggaagcacgu      540

gacaaaauga uuagauugcu gaccuucagg auccccuacu acgucggucc ucuagcccaa      600

ggggaggaaa cauccucuuu ugcuuggcuc gagcggaaga ccccagagaa agugacuccc      660

uggaacgcca cagaaguuau ugauuauagc gcguccgcaa ugaaguucau ccagcgcaug      720

auaaauuacg acaccuaucu gccuacggag aaagugcuuc caaagcauuc aauucuguac      780

cagaaguaua cuaucuuuaa cgaauugacc aaagucgccu acaaggauga gagaggaauu      840

aaacaccagu ucaguagcaa ggaaaagagg gagaucuuca aggagcucuu ucaaaagcag      900

cggaaaguga caguaaagaa acugcagcaa uucuuaucug cuaauuauaa caucgaagac      960

gccgagauuc ugggcguuga caaggcauuu aauuccucau acgcuacuua ccaugauuuc     1020

cucgaccuug ccaaacccaa caccgaacgc guggcggagc uguuggagca gccugaaaug     1080

aaugccaugu uugaggauau agucaagauc cuaacaauuu ucgaagaccg agagaugauc     1140

agaacccagc ugaagaaaua ucagagcgug cucggcgaug gguuuuucaa gaaacuggug     1200

aagaaacacu acacgggaug ggguaggcuu agugaacggc ugauaaacgg cauucgugac     1260

aagaaaacua auaagacaau cuuggauuau cucaucgacg augacgauuu uccauacaac     1320

cgcaacagaa auuucaugca acugauuaac gacgacucuu uauccuuuaa ggaggagcug     1380

gcaaaugaac uagcucucgc cgggaaccag agccugcuug aggucguuga ggcuuugccg     1440

ggaucgcccg ccaucaaaaa gggcauuugg cagacccuga aaauagugga agaacucauc     1500

gagauuaucg guuauaaucc uaagaacauc guaguggaga uggcaaggga aaaucagcgg     1560

acuaaccgau cuaaaccacg ccugaaggcc cuugaggaag cucugaaguc cuucgauuca     1620

cccuugcuca aagagcaacc ugucgacaau caggcgcugc agaaggauag auuauaccug     1680

uauuaccuac agaacgggaa agacauguac acaggagaag cacucgauau ugacaggcug     1740

agugaguaug auauagacca caucauucca cggagcuuua ucguggauaa uuccauugac     1800

aacaagguuc uugugucuag caaagagaau cgcuugaaga uggaugacgu ccccgaucaa     1860

aaggugguaa ucagaaugag gcguuacugg gaaaaacugc uccgggccaa ccugauauca     1920

gagcgaaagu ucgcuuauuu aaccaaacug gaacuuacgc cggaggacaa ggccagauuc     1980

auccagaggc aguugguuga gacccgccag auuacaaaac auguggcagc cauccucgac     2040

caauacuuua aucagccuga agagaguaag aacaagggca uucggaucau aacucugaaa     2100

uccagccuag ucucucaguu cagaaagacc uuuggcauuc acaaagugag ggaaaucaac     2160

aaucaucacc acgcucauga ugccuaucug aacggggucg uggcaauugc gcuccugaag     2220

aaauacccca aacuugagcc agaauuuguu uauggaaauu acacaaaauu uaacuuggcu     2280

acugagaaua aggccaccgc aaagaaagag uucuacucaa acauccugcg cuuuuucgaa     2340

aaggaggaau auaguuacga cgagaauggu gaaauauuuu gggauaaagc ccgacacauc     2400

ccucaaauua agaaagugau cagcucccau cagguaaaca uugucaagaa gguggagguu     2460

cagacaggcg gguucuauaa agagaccgug aaucccaagg gaaaaccaga caaguuaauc     2520

cagcggaaag cuggcuggga ugucucuaag uacggugggu uuggcucgcc cgugguagcc     2580

uaugcagugg cuuucauaua cgaaaaggga aaagcccgua agaaagcgaa ggccauugag     2640

ggcaucacua ucaugaaaca aagccucuuu gaacaggacc cuauuggguu ccuguccaac     2700

aagggauauu caaauguuac gaaguucauc aaacuuagua aguacacacu guacgaguug     2760

gaaaacggua gaaggcgcau ggucgcuucu cacaaagagg cacagaaggc caauagcuuu     2820

auucucccag agaaacuggu gacccuacug uucuaugcuc agcauuacga ugaaauagcc     2880

cacaaggagu ccuuugacua ugugaacgau caccucucag aguuccggga aauccuggac     2940

caagucauug auuuuagcaa uagauacacu aucgcagcga agaacaccga gaaaauugcc     3000

gaacuuuucg agcagaacca ggaaucuaca guucaguccu ugagucagag cuuuaucaau     3060

cugaugcaau uaaacgcuau gggcgccccg gcagacuuca aguuuuucga ugugauaauc     3120

cccaggaaac gauauccuuc ucucaccgag auuugggaau ccacuaucau uuaccaguca     3180

aucacagggc ugcgcgagac gcggaccaga auggcuacuc uuugggacgg agagcaauga     3240


<210>  2701
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2701
auggacgagg aagccaagau ccagcugagc aaagaguccu acgaagagga acucgagucu       60

cuucuggaga agucaggcga agaguucaga gauguguuuu ugcaagcuaa aaaggucuau      120

gacgcaauuu uacugaguga uauacuaagc accaaaaagc agaacuccaa ggccaaacuc      180

ucucugggga ugaucgaaag guacgacucg cacaagaaag aucuugagga gcugaagcag      240

uucguuaaag cgaauuugcc cgaaaagaca gcuaucuuuu ucaaggacuc aagcaaaaac      300

ggauaugccg guuacauuga uggcaagacu acgcaggagg acuuuuauaa auuccucaag      360

aaagaacuga augggauugc aggaagugag cgguuuaugg aaaaggugga ucaagagaac      420

uuccugcuca aacagcgcac cacagccaau ggcguaaucc cucaucaggu gcacuuaacu      480

gagcugaagg cuauaaucga acgacagaag ccauacuauc cgucccuuga ggaagcacgu      540

gacaaaauga uuagauugcu gaccuuuagg auccccuacu acgucggucc ucuagcccaa      600

ggggaggaaa caucuagcuu cgcuuggcuc gagcggaaga ccccagagaa agugacuccc      660

uggaacgcca cagaaguuau ugauuauucc gcgucagcaa ugaaguuuau ccagcgcaug      720

auaaauuacg acaccuaucu gccuacggag aaagugcuuc caaagcauag uauucuguac      780

cagaaguaua cuaucuucaa cgaauugacc aaagucgccu acaaggauga gagaggaauu      840

aaacaccagu uuagcucuaa ggagaaaagg gagaucuuca aggaacucuu ccaaaagcag      900

cggaaaguga caguaaagaa acugcagcaa uuuuuauccg cuaauuauaa caucgaagac      960

gccgagauuc ugggcguuga caaggcauuc aauucaagcu acgcuacuua ccaugauuuu     1020

cucgaccuug ccaaacccaa caccgaacgc guggcggagc uguuggagca gccugaaaug     1080

aaugccaugu ucgaggauau agucaagauc cuaacaauuu uugaagaccg agagaugauc     1140

agaacccagc ugaagaaaua ucagagugug cucggcgaug gguucuuuaa gaaacuggug     1200

aagaaacacu acacgggaug ggguaggcuu ucugaacggc ugauaaacgg cauucgugac     1260

aagaaaacua auaagacaau cuuggauuau cucaucgacg augacgauuu cccauacaac     1320

cgcaacagaa auuuuaugca acugauuaac gacgacuccu uaucguucaa ggaggagcug     1380

gcaaaugaac uagcucucgc cgggaaccag agccugcuug aggucguuga agcucuacug     1440

ggaucuccgg ccaucaaaaa gggcauuugg cagacccuca aaauagugga ggaacugauc     1500

gagauuaucg guuauaaucc caagaacauc guaguggaga uggcaaggga aaaucagcgg     1560

acuaaccgau ccaaaccucg ccuuaaggcc cuggaggagg cuuugaaguc auuugauagu     1620

ccacuccuga aagaacaacc cgucgacaau caggcguuac agaaggauag acuguaccua     1680

uauuaccucc agaacgggaa agacauguac acaggagaag cacuggauau ugacaggcuu     1740

agcgaguaug auauagacca caucauuccu cgguccuuca ucguggauaa uucuauugac     1800

aacaagguuu uggugagcuc aaaagagaau cgccugaaga uggaugacgu cccagaucaa     1860

aaggugguaa ucagaaugag gcguuacugg gaaaaacucc ugcgggccaa cuuaauaagu     1920

gagcgaaagu uugcuuaucu gaccaaacuu gaauugacgc ccgaggacaa ggccagauuc     1980

auccagaggc agcucguuga gacccgccag auuacaaaac auguggcagc cauccuggac     2040

caauacuuca aucagccgga agaguccaag aacaagggca uucggaucau aacucuaaaa     2100

agcucucugg ucucacaguu uagaaagacc uucggcauua acaaagugag ggaaaucaau     2160

aaccaccauc acgcucacga ugccuaucuc aauggggucg uggcaauugc gcugcuuaag     2220

aaauacccua aguuggagcc cgaauuuguu uauggaaacu acacaaaauu caaucuggcu     2280

acugagaaca aggccaccgc aaagaaagag uuuuacagua auaucuuacg cuucuuugaa     2340

aaggaggaau auagcuacga cgagaacggu gaaauauucu gggauaaagc ccgacauauc     2400

ccacaaauua agaaagugau cuccucucac cagguaaaua uugucaagaa gguggagguu     2460

cagacaggcg gguuuuauaa agagaccgug aacccuaagg gaaaacccga caagcucauc     2520

cagcggaaag cuggcuggga ugucucgaag uacggugggu ucggcagccc agugguagcc     2580

uaugcagugg cuuuuauaua cgaaaaggga aaagcccgua agaaagcgaa ggccauugag     2640

ggcaucacua ucaugaaaca aucccuguuc gaacaggacc ccauuggguu ucuuucaaau     2700

aagggauaua guaacguuac gaaguucauc aaacugucua aguacacauu guacgagcuc     2760

gaaaauggua gaaggcgcau ggucgcuagc cauaaagagg cacagaaggc caacuccuuc     2820

auucugccug agaaauuggu gacccugcuc uaucacgcuc agcauuacga ugaaauagcc     2880

cacaaggagu cauuugacua ugugaaugau caccugagcg aguuccggga aauccuugac     2940

caagucauug auuuuucuaa cagauacacu aucgcagcga agaacaccga gaaaauugcc     3000

gaauuguucg agcagaauca ggaauccaca guucagaguc ugagccaauc uuuuaucaac     3060

uuaaugcagc ucaaugcuau gggcgcccca gcagacuuca aguuuuucga ugugauaauc     3120

ccgaggaaac gauaucccuc ccugaccgag auuugggaau caacuaucau uuaccagagu     3180

aucacagggc uucgcgagac gcggaccaga auggcuacuc ugugggacgg agagcaauga     3240


<210>  2702
<211>  3243
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2702
augaagacca gcuucgccuu ugacaucggc acaaacucca uuggguggug cgugcuggcu       60

cucgauaaug caaaccuucc cuacagaaua cuggacgccg gagucaggau cuucucugau      120

ggucgggagc cuaaaucagg cacgaguuug gcggaagggc gccgauuagc ucguggaaug      180

agcagaaggc gggaccgcua uaagagaagg cggaaagccg uuauucgcac ucugaucgag      240

uacggccuaa ugccagauga cccggcagcc cggagagcuc ucguggcaga aaccaaugau      300

aaggcccccg guaaaauugc uuccgacgua uaugcccuga gggcgcgggc acuugaugac      360

aagcugccuu ugcaccauau cgggcgugcc cucuuucaca uaaaccagcg cagaggauuc      420

aagucuaaua ggaaaacaga uaagcgggac aacgaucaag gcaaaaucgc uucgggcauu      480

ucacgacugc gcgacaagcu ggccagcgag gggaugagaa cucucggaga auacuuaaau      540

cauaggcacc aggcagguaa cugggugcgg guccgcccaa guagagugcc cggcaaagac      600

gauaaggagg aggacgggua uggauuuuac ccugaaaggg cucugcuuca ggaugaguuc      660

aaugccaucu ggaccgcgca ggccgcacau uauccagacg uuuugacaga agaacgucgg      720

acccaccugg agacuauuau cuuuuuccaa cgaccccuaa agccugugca ggucggcaaa      780

uguuccuuua acccagguga agagagacuc ccgagggcuc acccccuguu ccaggaauuu      840

cgccuuuaca aggaggugaa ugaacuggag uugguagauc ggaaccagga ccaucacaaa      900

cucucucugg aucaaagaga cgccuuaaua gcucagcuga agacacagag ggaggccgca      960

uacagcgccc uccgcaaaac cgcucgagcg ccucuuggga cgcgguucaa uaaggaaacu     1020

gagucccgua ccaaaaugaa gggagaugaa guugcagccg agcugucagc uaagacaaga     1080

uuuggccagg ccuggauuag uaugccauug gaagagaggu ggaucauugu ggacacucua     1140

cgccgggucg aggauccccu ggaacucacc gcauggcugg agcaacauua uggccuugac     1200

ggggaacaca ucgacgccau ccuggcugcc agauugccug agggauacgg uaggcucggc     1260

gaaacagcac ugaccgagau uuuaguggag cugaaagcgg cugugauaag cgaagccgag     1320

gcagcccgac gcgcugggua ugaucacgcc cuacuccagc ggggagcagg ccuggaagcc     1380

cuuccaaagu accaggaggu caucgcuaga aggauucccc cggguacggg gaacccugac     1440

gaucccuaug acguugagaa aggccguauc acuaauccaa cagugcauau aggauugaac     1500

caacugcaga agguacucaa cgcgcugauu cgccgguacg gcaaaccuga acagaucgcc     1560

aucgagcuug cuagagaacu gaaguugaau gaugagcaga agagggagaa aaacauugag     1620

aucgcacgaa auacccgggc cgcugagcaa cgcucugcca agcucgugga acuggggguc     1680

cccgacacug gauauaacag aguguuacug aaacuauggg aggaacucaa ucugagggca     1740

ccagagaacc ggguuugcau uuacuguggu gaacccauaa auaucgagac acuuuucucc     1800

ucagcggugg auauugacca caucuugccu uggagcaaga cccuggauga cagucaggcc     1860

aaccgacucg ucugccaugu ggcuuguaau agacagaaag ccaacuacgc accagcugag     1920

guaccgcagu ggcaaggccg cuaugaugac auccuggcca gggcaaauac guuaccccug     1980

aacaagcguu ggcgguucgg gagagaugcc augaagaaau uugcugacga agagaccuuc     2040

cuugcgaggc aguugacaga cacucaguac cucucucgcc uggcccacgc auauauggcu     2100

gcccuauacc cugaugagga accagacgcc cagggaguuc uggcucgaaa gaaucaugug     2160

agagucauua ccggccggau gacagaaaug cucaggcgcc aauggggucu gaacucccuu     2220

uuggcagauc acaaucagaa aaaccguaac gaccacagac aucacgccau agaugcggcu     2280

guggucgggg ugacuacccg ggcccuguua cagaggaucg caacaguugc cggcggacgc     2340

gaggcucagg cacucgagga aaccauuggc aagaucgagc ccccuuggcc aggguuucga     2400

gacgaacugc aagccgugau uaaaagaauc guagucagcc auaagcccga ucacggaacu     2460

cuuccgaagc cuggucaggg cgggcagacg gcgggacaac ugcacaauga cacagcuuau     2520

ggcuugaccg gugagacuga uucgcggggg accccaauag ugguuaggcg caaacccuuc     2580

acaucucuca ccgagaagga ccugccuucc aucagagaua ggcagcuagc ccaggcacug     2640

ggcucagcca uugcuggacg gaguggcaaa gaacuccagg ccgcacugga gcaauuucgu     2700

gaccgcgaug acuacaagag cauccgacau gugagagucg uggaaccccu uaacguaauu     2760

ccaaucaggg acgggacugg acgggcuuau aaagccuaca agggcgaugc gaauuaccgc     2820

uaugacgugu gggaguugcc uaacgguaaa uggaugcacg aaguugucuc cauguucgau     2880

gcccaugaca gaucuuggac aagcgcagug cacauacauc accccgcugc caggcgggug     2940

cugucauuaa agcagaauga uauggucgcu uacgaccacc cacaggaugg gccgacgauu     3000

ggacgaguug ugaaguuuag ucaguccggc caaaugaccu ucgcaccuca uaaagaggcc     3060

ggugcccuca aggcuagaga cgcggauccc gaggaccgcu uuagguauuu cuacaaaagc     3120

ggcucugcaa ugaaggauau cgggcugcga caggugcgug uagacgaaau uggaagaguc     3180

uuugacccag gccccaggga uaaagaggcc cgcaacgcuc ggaagaagaa aucaacugac     3240

uga                                                                   3243


<210>  2703
<211>  3243
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2703
auggccaaga gcuucgcuuu ugacaugggc accaacucca ucgggugggc agugcuggga       60

cucgauucug ccggugaccc cgauucaauu gagggcgcgg gggucagaau auucagugac      120

ggaagggaac cuaaaagcgg cgcuucccuu gccgaggguc ggcgcaucgc acgagggaug      180

ucucguagaa gggaacggua caaggcccgc agaaaagcuc ugauuaggac auugggagau      240

uauggcuuaa ugccacugga gcgggcagcc cgccgagcuc uaguugccga aacuaaugac      300

agaucguuug gccucguggc gcaggaugua uacgcacuga gggcccgggc ucuuguggag      360

aagcugccgc ccuaucacuu ggggcgugcc cucuuccauc ugaaccaacg cagaggauuu      420

aaaucaaaua ggaagacgga cggucgggau aacgagcugg gcaugaucgc aaccgggauu      480

gcugcccucg acgcgcaguu aaauggacga acacugggcc aguaccuuca cgaaaugcgc      540

ggugccgauc cuagacagag ggggcaaguc cgggugcgcc cacauuugga gaucaacgac      600

aagggaagag aaauagcagc uuauggcuuc uacccccaga gggccaugcu ggagcgugag      660

uuuaaugcua ucugggccgc acaggccccu caccauccag aacuacucag cgaggaacgg      720

cgagcucacc uguuccaggu uaucuuuuau caaagacccc uuaaacagcc ugauccaggg      780

aggugcaguu ucaacccggc ggagccccgc cuggcaaagg cccacccuuu guuucagcgg      840

uucagacucg ugaaagaaau uaaugagcug aggauuguca cuggguccaa cucucaggga      900

gugccauuaa cccuggagca acgcgacauc cuccuuacac uguugcgaga caagaaagcu      960

agcuccuuua agacccuacu ggccaagaua auuaaagguc agcccgaagc acgguucaau     1020

aaggccucag auagucguga caaaaugaag ggcgaugagc ucgcugccac uaugagcgac     1080

aaaacaagau ucgggcaggc augguuugcg augccugaag cugagcaaug gaggaucauu     1140

gccaagcugc gcgauaucga cgauccacuu gaacugaacg caugguugga gucugaguac     1200

ggacucgccc ccgaacggag agacgcugua gccaaugcac uguuaccgca gggcuacggu     1260

aggcuggggg agaccgcucu acgaacgaug cucgauuauc uggaagccgc gguuaucucc     1320

gaggccgaag cugcagccca ggcuggcuac gaccaugccc uugcacgccg gggagcgggc     1380

uuggaucagc ugccuaaaua ucaagaggug cucgggagaa ggauuccccc aggaucaggu     1440

gagccuaacg accccuacga cauacguaug ggccgcauca gcaauccaac uguccacauu     1500

gcccugaacc agcuucggag aguggugaac gcucugauca ggaaguaugg gaagcccgcc     1560

gaaauagcaa uugaguuggc ucgagaucuc aaacugagug aagaacagaa gcgggagguc     1620

aaugccacca ucgccaaaaa cacacgcgac gcagaaagaa gggcuguucg guuagcggau     1680

gagggaaucg acaauacugg ccauaaccga gcccugcuaa gauucuggga ggagcucgca     1740

gcugauccuc uggaccgcgu guguguauac ugcggugggg gcauuggagc cgccucucuu     1800

uuuaauggcu ccguggauau cgaccacauu uugccaugga gcaggacccu ggaugacucg     1860

cgugcuaacc ggauacucgu ccacaauucu uguaacagac ugaagaggaa uuucgcaccg     1920

uuugaagugc ccgaguggcg cgagaucuau caggauauuu uaguucgagc cgcgaaacug     1980

ccugcuaaca aguccuggag auucgcccca gacgcaaugg aucgguuuga aggggagaag     2040

gacuuccuug ccaggcaauu gacagacacc caguaccucg cucgcauggc acaugaauac     2100

cuggccgcgc uauaucccga ugaggaaccu gacgaguggg gaguguuugc ucgugguaau     2160

cacgucagag ugguaccagg ccggcugacg gagaugcuca ggcgcgccug ggggcugaac     2220

gcaauccuuu cagaugacaa ucgaaagaac agaaaugauc aucggcacca ugccaucgac     2280

gcuguugugg ucggagugac uacaagggau auguugaaca aaauuaguca ggccgcaggc     2340

cgcggugcug acaugggggc cgcggaugcc cugagaaggu ucgauccccc guggccuagc     2400

uuucaccagg caguccgggc ugccguggaa cguauauuag uuucccaccg cgcugaccau     2460

ggcacccucc caggaacuga cggccaaggg ucuacagcag gacagcugca caacgauacc     2520

gccuacggcc uuacggguga gacccgaaaa gggcuggccg ugguagucag aaggaagccc     2580

uauuugagcc ucgaaccucg ggacaucgag acaauucgcg acccccagcu gagagaucau     2640

cuagcuacug aaaccuacgg acuggcgggc gcagaguuca cagccgcucu caaaaggcug     2700

cagcaaacug acaaguuuua ugccgguauc cggcgaguga gaguuaccga ggcacuuuca     2760

gugauuccaa uccgcgaugc cacaggccac gcuuacaagg cguauaaagg ggacgccaau     2820

uucagguacg augucuggga auugccuaac ggaaaguggg ugcccgaggu aauaaguaug     2880

uucgacgcac acgaucgggg cugggugucc cagguucaug cugccaaucc agcagcccgu     2940

agaguccuga gcuuaaggca gaacgacaug guggcuuacc aacacccgga acaggggcuc     3000

guguauggac gcgucguuaa auuuggccag ucuggucaga ugaccuucgc cgcgcaucac     3060

gagggcggau cacugaagag ucgggaugca gaccgagaug acccuuuuaa auacuucuau     3120

aagagcgccg gcacuaugaa ggacaucggu cuuagacaaa uuagggugga ugagaucggc     3180

cgcgugcagg accccgggcc acaggaucgg gacuccaaaa gaguacguaa gucugauagg     3240

uga                                                                   3243


<210>  2704
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2704
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagacgaga acccaaucug ccuuauugau      120

cuggguguua gaguguuuga aagggcggag guaccgaaaa ccggcgacuc cuuggcuaug      180

gcccggcgcu uagcacgauc ugugcguaga cugacaaggc ggcgcgccca cagacuacuc      240

agggcucggc gccugcuuaa gcgagaaggg guccugcagg cagccgauuu cgacgagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gagcugccgc gcuggauagg      360

aagcucacgc cauuagagug gagugcagug cugcuucauu ugauaaaaca ccggggcuau      420

cugagccagc guaagaauga aggugagacc gccgacaagg aacuaggggc ucuccugaaa      480

ggaguugccg auaacgcaca ugcccuucag acaggcgacu uucgcacucc cgcuaagcug      540

gcguugaaua aauucgagaa ggaguccggc cacaucagaa accagagggg agauuacucu      600

cauaccuuuu cgcggaaaga ccuccaagcc gaacugaauu uacuguucga gaagcagaag      660

gaauuuggaa acccucacau uucagauggu cucaaagagg gcaucgaaac acuucugaug      720

acccagcgac cagcauugag cggggacgcu gugcagaaga ugcuaggaca cuguacuuuc      780

gagcccacag agccuaaagc cgcuaagaau accuauacgg ccgaacgcuu ugucuggcug      840

acuaaacuca acaaucugag aauuuuagag caaggcagug aacagccacu gaccgacaca      900

cagagggcaa aguugaugga ugagccguac cggaaaucca agcucacuua ugcccaggcu      960

cgcaagcugu uaggucugga agacaccgcg uucuuuaaag ggcuaagaua cggaaaggau     1020

aacgcagagg ccucuacacu cauggagaug aaggcuuauc augccaucag cagggcacug     1080

gaaaaagagg gccuuaaaga caagaagucc cccuugaauc ugucaccuga acuccaagau     1140

gagauaggca ccgccuucag ucuguuuaaa acggacgaag auauuacugg gcgucuuaag     1200

gaccgggugc agccagagau ccuggaggcu uugcucaaac acauuagcuu cgauaaguuc     1260

guacagaucu cucugaaagc cuuacgaaga aucguucccc ugauggaaca aggaaagagg     1320

uacaacgagg caugcgcgga aauuuacggu gaccauuaug gcaagaaaaa cacagaggag     1380

aagauauacc uaccuccaau ccccgcugau gaaauucgca auccgguggu ccuccgggcc     1440

cugucccagg caagaaaagu gaucaacggg guggucaggc gcuauggaag cccugcccga     1500

auacacauug agaccgcucg ggaaguuggc aagucauuua aagaccguaa ggagaucgaa     1560

aagagacagg aggagaauag gaaagaccgc gaaaaggccg cagcuaaauu ccaggaguac     1620

uuucccaacu ucguggguga accaaagagu aaagauaucc uuaaguugcg gcuguaugag     1680

caacagcacg ggaaaugucu cuacucuggc aaggaaauua aucugggaag auuaaacgag     1740

aagggcuaug uagagaucga ccaugcccug ccuuuuucca ggacuuggga ugacagcuuc     1800

aauaacaaag ugcuugucuu ggggucggaa aaucagaaca agggaaauca gacacccuac     1860

gaguacuuua acgguaaaga uaauucucga gaauggcaag aguucaaggc gcgcguggaa     1920

accucccggu uuccaagaag uaaaaagcag aggauucucc ugcagaaguu cgacgaggau     1980

ggcuuuaaag agcguaaccu aaaugacacg cgcuauguua accgguuccu gugccaguuu     2040

guggccgaua gaaugaggcu caccgggaag gggaaaaagc gagucuucgc uucaaacggc     2100

caaauaacaa aucugcuucg ggguuucugg ggguugcgca aagugagagc agaaaacgac     2160

aggcaccaug cccuggaugc uguaguugug gccuguagca cugucgcaau gcagcagaag     2220

aucacccggu uugugcgaua caaggagaug aaugcguucg acggcaaaac aauugauaag     2280

gaaacuggag aggucuuaca ccagaaaacc cauuuucccc aaccuuggga guucuuugcc     2340

caggaaguga ugaucagagu uuucggcaag ccagacggga aaccggaguu ugaagaggcu     2400

gacacacccg aaaagcuccg cacccugcuu gccgagaagc uguccucuag gccugaggca     2460

gugcacgaau auguaacucc auuguucguc agccgugcuc ccaaccggaa aaugucagga     2520

cagggucaca uggagacggu gaagagugcc aaaagacucg augaaggcgu uuccgugcug     2580

agggucccuc uaacacaacu gaagcucaaa gaccuggaga agauggugaa ucgcgaacga     2640

gagccaaaac uuuacgaggc auugaaggcc agacuggaag cucauaagga ugaccccgcg     2700

aaagccuuug cagagccguu cuauaaguac gauaaagcug ggaaccggac ccagcaggua     2760

aaggccguga ggguugaaca gguccagaaa acuggagugu gggugcgcaa ucacaacggc     2820

aucgccgaca augcuaccau gguccguguu gauguguuug agaaggguga caaguauuac     2880

uuagugccua uuuacagcug gcaaguagca aaagggauac ucccagauag agccgucgug     2940

gcguaugcug acgaagagga uuggacaguu aucgacgagu cuuuccgguu uaaguucgug     3000

cuguacucaa acgaucuuau uaaaguccag cugaagaaag acaguuuccu aggcuauuuu     3060

agcggacucg acagggccac cggcgcaauc ucccugcgcg aacaugauuu ggagaagucu     3120

aaggggaaag acggaaugca ccgaauuggc gugaagacug cccugucguu ccagaaauac     3180

caaaucgaug aaauggguaa ggagauaaga cccugccggc uuaaaaagag gccucccguu     3240

cgcuga                                                                3246


<210>  2705
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2705
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagacgaga acccaaucug ccuuauugau      120

cuggguguua gaguguuuga aagggcggag guaccgaaaa ccggcgacuc cuuggcugcc      180

gcacggcgcu uagcccgauc ugugcguaga cugacaaggc ggcgcgcuca cagacuacuc      240

agggcacggc gccugcuuaa gcgagaaggg guccugcagg ccgcugauuu cgacgagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gaagugccgc gcuggauagg      360

aagcucacgc cauuagagug gagcgcagug cugcuucauu ugauaaaaca ccggggcuau      420

cugucccagc guaagaauga aggugagacc gccgacaagg aacuaggggc ucuccugaaa      480

ggaguugccg auaacgcaca ugcucuacag acaggcgacu uucgcacucc cgccgagcug      540

gcguugaaua aguucgagaa agaaucuggc cacaucagaa acaggcgggg agauuacucg      600

cauaccuuuu cacgaaagga ccuccaggcc gagcuggauu uacuguucga aaaacaaaag      660

gaguuuggaa auccucacau uagcgacgac cucaaggaag guaucgagac acuucugaug      720

acccagcgcc cagcauugag uggcgaugcu gugcagaaaa ugcuagggca cuguacuuuc      780

gagcccacag aaccuaaggc cgcuaaaaac accuauacgg ccgagagauu ugucuggcug      840

acuaagcuca auaaccugag gauucuugaa cagggauccg agcggccacu gaccgacaca      900

gaacgcgcaa cuuugaugga ugagccguac agaaaaucua agcucaccua ugcccaagcu      960

aggaaacugu uagagcugga cgauacagcg uucuuuaagg gccuacguua cgguaaggac     1020

aaugcagaag ccagcacccu cauggagaug aaggcuuauc augccaucuc ccgggcacug     1080

gaaaaagagg ggcuuaaaga uaagaaauca cccuugaacc ugaguccuga acuccaggac     1140

gagauaggaa cggccuucag ccuguuuaag acugaugagg acauuacagg ccgacuuaag     1200

gauagagugc agccagaaau ccuggaggua uugcucaaac acauuucuuu cgacaaguuc     1260

guucagaucu cccugaaggc uuuaaggcgc aucgugcccc ugauggaaca aggcaaacgg     1320

uacgacgagg ccugcgcaga gauuuacggg gaucaugacg ggaaaaagaa uaccgaagag     1380

aagauauauc uaccuccaau ccccgcggau gaaauuagaa acccggucgu gcucagggcu     1440

cugucacagg cccgcaaagu gaucaacgca gucguucgac gguacgguag cccugcccgu     1500

auacacauug agacugcuag agaagugggc aagaguuuua aagacaggaa ggagaucgag     1560

aaacgccagg aagagaaucg gaaggauaga gaaaaggccg cagcuaaauu cagggaguau     1620

uuucccaacu ucguagggga accaaagucu aaagacaucc uuaaguugcg acuguacgag     1680

caacagcacg gaaaaugucu cuauuccggc aaggagauua aucugggucg cuuaaacgaa     1740

aaaggguacg uggagaucga ucaugcccug ccuuuuagcc ggacauggga cgauucguuc     1800

aauaacaagg uccuuguguu gggcucugaa aaucagaaca agggaaauca gacccccuau     1860

gaguacuuua acggcaaaga caauuccaga gaauggcaag aguucaaggc gaggguugag     1920

acgucacguu uuccacgcag uaaaaagcag cggauucucc ugcagaaauu cgaugaagag     1980

ggguuuaagg aaagaaaccu aaaugacacc agguacguga accgauuccu gugccaguuu     2040

gucgccgauc acaugcuccu gacaggaaag gguaaacggc gcguguucgc uagcaauggc     2100

caaauaacua accuuuugag aggguucugg ggacugagga agguacgggc agagaacaau     2160

cgacaucacg ccuuagacgc uguugugguc gccuguucca ccguggcaau gcagcagaaa     2220

aucacaagau uuguccgcua uaaggagaug aacgcguucg acggcaaaac uauugauaag     2280

gaaaccggug aggugcucca ucagaagaca cacuuucccc aaccuuggga guucuuugcc     2340

caggagguua ugaucagggu guucgggaaa ccagacggca agccggaauu ugaggaggcu     2400

gauacccccg aaaaacugcg uacucuucug gccgagaagu ugucuagccg gccugaagca     2460

guacacgagu acgucacgcc acucuucgug ucaagagcuc ccaauaggaa aaugagugga     2520

cagggccaua uggaaacagu uaaguccgcc aagcgccugg acgagggggu gagcguccua     2580

cgagugccuc ugacccagcu caaacugaag gaucuugaga aaaugguaaa cagagaacgg     2640

gagccaaagu uguaugaagc acugaaagcc agguuagagg cucacaagga cgaucccgcg     2700

aaagccuuug cagaaccguu cuacaaguau gacaaggcug gaaaucgcac ucaacaggug     2760

aaagccguuc gugucgaaca ggugcaaaag accggugugu gggucagaaa ccauaauggc     2820

aucgccgaua acgcuacaau gguucgggug gacguguuug agaaagcagg gaaguacuau     2880

cucguaccua uuuacucuug gcaggucgcc aaaggaauac ugccagauag ggcggugguu     2940

gcuuacgccg acgaagaggg cuggaccgug aucgaugaau cauuccgcuu uaaguucguc     3000

cuuuauagua augaccugau uaaggugcag cuuaaaaagg acagcuuccu cgguuacuuu     3060

uccgggcugg aucgagcaac uggcgccauc ucuuugagag agcacgaccu ggagaaaucg     3120

aagggaaaag auggcaugca ccggauuggg guuaagacag cucucagcuu ccagaaguau     3180

caaaucgacg aaaugggaaa agagauaagg uuaugccgcc ugaagaaaag acccccugug     3240

agguga                                                                3246


<210>  2706
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2706
augaccgccu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgcu       60

agcgugggau gggcaauggu cgagauugau gaagaggaaa acccaaucag acuuauugac      120

cuggguguua ggguguuuga gcgggccgaa guaccgaaaa caggcgauuc cuuggcggcu      180

gcccgccgau uagcacguuc ugugagaagg cugacucggc gcagagccca caggcuacuc      240

cgggcucgcc gacugcuuaa gagagagggg guccugcagg cagccgacuu cgaugagaau      300

ggauugauca aaucacuccc caacacgccu uggcaacuga gggcugccgc gcuggaccgg      360

aagcucaccc cauuagaaug gagugcagug cugcuucauu ugauaaaaca ccguggcuau      420

cugagccagc gcaagaauga gggugaaaca gccgauaagg agcuaggggc ucuccugaaa      480

ggaguugccg acaacgcaca ugcccuucag acuggcgauu uuagaacccc cgcugagcug      540

gcggugaaua aguucgaaaa agaguccggc cacaucagga accagcgagg ggacuacucu      600

cauacauuuu cgcggaagga uuugcaagcc gaacucgucc uguuauucga gaaacagaag      660

gaauuuggaa auccucacau uucagacggu cugaaggagg gcaucgagac ccuccuuaug      720

gcacagcgcc cagcucugag cggggaugcc gugcagaaaa uguugggaua uugcacuuuc      780

gaacccacag agccuaaggc ugccaaaaac accuacacgg cagaaagauu uauuuggcua      840

acuaagcuga auaaccucag gauccuggag caaggcagug aacggccacu uaccgacaca      900

gagcgcgcca cucugauaga caugccguau aaaaauaagu ugaccuacgc uaaagcgaga      960

gagcuccugg guuuagauga cacagcauuc uuuaaggggc ugagguaugg aaaggauaac     1020

gccgaauccg cuacccuaau ggagaugaaa gccuaccacg caauucgucg ggcccucgaa     1080

aaggagggcc ugaaagacaa gaaaucuccc cuuagcuugu ccccugaacu gcaggaugag     1140

aucggcacgg cuuucucacu cuuuaagacu gacgaggaua uuacagggcg acugaaggga     1200

ugucuucagc cagaaauccu ggacgccuug cucaaacaua ucaauuucga uaaguucgua     1260

cagauuaguc ugaaagcauu aagaaggaua guuccccuga uggagcaagg uaagcgcuac     1320

gacgaagcgu gcgcugagac cuauggcgau cacuacgggg agaaaaacag cgaagagaug     1380

cuauaucucc cuccaauccc cgccgacgaa auucggaacc cggugguccu gagagcacuu     1440

ucucaggcca ggaaggugau caauggagug guccgccgau acggcucccc ugcucggaua     1500

cauauugaga cugcccguga aguugguaag ucauuuaaag acagaaagga gaucgagaaa     1560

aggcaggaag agaaccgcaa ggaucgggaa aaagcagcug ccaaguucag agaguauuuu     1620

cccaauuucg ugggggaacc aaagagcaaa gacaucuuga agcugaggcu cuacgagcaa     1680

cagcacggca aaugucugua uaguggaaag gcgauugaua uccgacgcuu aaacggcaaa     1740

ggguacguag agauugacca cgcccugccu uuuucucgga caugggauga cuccuucaau     1800

aacaaggugc uugucuuggg aagcgaaaau cagaacaaag guaauagaac ccccuacgag     1860

uaucucaacg gcaaggauaa uucgagggaa uggcuggagu uuaaggcucg uguggaaacg     1920

ucucgcuucc cacauuccaa aaagcagcgg guucuacugc aaaaauuuga cgagaagggg     1980

uucaaagaga gaaaccucaa ugauaccagg uacgugaacc gauuucugug ccaguucguc     2040

gcagaccaca ugcagcuuac aggaaagggc aagcggcgcg uguuugccuc aaauggucag     2100

auaacuaacu ugcugagagg guucuggggc uuacauaaag uaagggcuga aaacgaucgg     2160

caccaugccc ucgacgcagu uguggucgcg uguaguaccg uggccaugca acagaagauc     2220

acaugcuucg uccgauauaa agagaugaau gcuuuugaug gaaagacugu ggacaaagaa     2280

accggcgagg uucugcacca gaagacacac uucccccagc cuugggaguu uuucgcccaa     2340

gaagugauga uuagaguauu ugggaagcca gacggaaaac cggaguucga agaggcagau     2400

acccccgaaa aacuucgcgc ucuguuggcc gagaagcuca gcuccaggcc ugaggcaguc     2460

caugaauacg ugacuccacu guuuguuucu cgugccccca accggaagau gagcggugcu     2520

cacaaagaca cgcuaagauc agcgaagagg uucgugaagc auaaugagaa aaucaguguc     2580

aagcgcgugu ggcugacaga aaucaaacuc gccgaucugg agaacauggu aaauuauaag     2640

aacggccgag aaauugagcu uuacgaggca uugaaagcua gacugggggc cuauggaggc     2700

aaugccaagc aggcuuuuga cccuaaagau aacccauucu acaagaaggg ugggcaguua     2760

gugaaagcag uucgggucga aaagacccag gaguccggcg ugcuccugaa uaaaaagaac     2820

gccuacacua uagcggacaa uggagauaug gugagggucg acguguuuug uaaaguugau     2880

aagaagggca aaaaccaaua uuucaucgug cccauuuacg cuuggcaggu agccgaaaau     2940

auccuuccgg acauugauug caagggguau cgcaucgacg auagcuacac cuuuuguuuc     3000

ucucugcaca aauaugaccu aauagcauuc cagaaggacg agaaaucaaa ggucgaauuu     3060

gccuacuaua ucaacugcga uaguagcaau ggacguuucu accucgcuug gcacgacaag     3120

ggcuccaaag agcaacaguu uagaauuucu acacagaacc ugguguugau gcagaaguac     3180

caaaucgaug agcuggguaa agaaauucgg ccuuguaggu uaaagaaacg cccacccguu     3240

cgauga                                                                3246


<210>  2707
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2707
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagacgaga acccaaucug ccuuauugau      120

cuggguguua gaguguuuga aagggcggag guaccgaaaa ccggcgacuc cuuggcugcc      180

gcacggcgcu uagcccgauc ugugcguaga cugacaaggc ggcgcgcuca cagacuacuc      240

agggcacggc gccugcuuaa gcgagaaggg guccugcagg ccgcugauuu cgacgagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gaagugccgc gcuggauagg      360

aagcucacgc cauuagagug gagcgcagug cugcuucauu ugauaaaaca ccggggcuau      420

cugucccagc guaagaauga aggugagacc gccgacaagg aacuaggggc ucuccugaaa      480

ggaguugcca acaaugcaca ugcccuucag acaggcgauu uucgcacucc cgcugagcug      540

gcguugaaca aguucgagaa agaaucuggc cacaucagaa aucagcgagg ggacuacucg      600

cauaccuuuu cacggaagga ucuccaagcc gagcugauuu uacuguucga aaaacagaag      660

gaguuuggaa acccucacgu gagcgguggc cucaaggaag ggaucgagac acuucugaug      720

acccagcgac cagcauugag uggagacgcu guccagaaaa ugcuaggcca cuguacuuuc      780

gagcccgccg aaccuaaggc ugccaaaaau acauauaccg cagagcgcuu uauuuggcug      840

acgaagcuca acaaucugag aauccuugaa caagguuccg agaggccacu gacugauacc      900

gaacgggcca cauugaugga cgagccguac cgcaaaucua agcucacuua ugcucaggcg      960

agaaaacugu uagggcugga ggacaccgca uucuuuaagg gacuaaggua cggcaaggau     1020

aacgccgaag cuagcacacu cauggagaug aaagccuauc augcaauauc ccgugcccug     1080

gaaaaggagg gccuuaaaga caagaaauca cccuugaauc ugaguccuga acuccaggau     1140

gagauuggga ccgcuuucag ccuguuuaag acggacgagg auaucacugg acggcuuaag     1200

gacaggauuc agccagaaau ccuggaggcc uugcucaaac acaucucuuu cgauaaguuc     1260

gugcaaauuu cccugaaagc auuaagaagg auaguacccc ugauggaaca ggguaagcgc     1320

uacgacgagg cgugcgcuga gaucuacggc gaucauuaug ggaaaaagaa cacagaagag     1380

aagauuuacc uaccuccaau ccccgccgac gaaauacgga acccgguugu gcucagagca     1440

cugucacagg ccaggaaagu cauuaaugga guggugcgcc gauauggcag cccugcucgg     1500

auccacaucg agaccgcccg ugaagucggu aagaguuuua aagauagaaa ggagauugag     1560

aaaaggcaag aagagaaccg caaggaccgg gaaaaggcag cugccaaauu cagagaguac     1620

uuucccaauu ucguugggga accaaagucu aaagacaucc uuaaguugag gcuguaugag     1680

cagcagcacg gcaaaugucu cuacuccgga aaggagauua accugggccg auuaaaugaa     1740

aaaggguaug uggagauaga ucaugcgcug ccuuuuagcc gcacuuggga cgauucguuc     1800

aacaauaagg uacuuguguu gggaucugaa aaccagaaua aggguaacca aacacccuac     1860

gaguacuuua auggcaaaga caacucccgg gaauggcagg aguucaaggc cagagucgag     1920

accucaaggu uuccacguag uaaaaagcag cgcauccucc ugcagaaauu cgaugaagac     1980

ggguuuaagg agcggaaucu aaacgauacg agauauguga auagguuccu gugccaauuu     2040

guugcugacc gaaugcggcu caccggaaag ggcaaaaagc gcguguucgc aagcaacggu     2100

cagauuacaa accugcuuag aggguucugg ggcuugagga aaguccgggc cgaaaaugau     2160

cgacaccaug cucuggacgc cgugguaguu gcauguucca cuguggcgau gcagcagaag     2220

aucaccagau uuguccgcua caaagagaug aacgccuucg auggaaagac aaucgacaag     2280

gagacuggcg aaguguuaca ccagaaaacc cauuuucccc agccuuggga guucuuugcu     2340

caggaaguca ugauuagggu guucgggaag ccagauggaa aaccggaguu ugaagaggcc     2400

gacacacccg agaagcuccg uacccugcuu gcagaaaaac ugucuagccg gccugaggcu     2460

guucacgaau augugacucc auuguucgua ucaagagccc ccaauaggaa gaugaguggu     2520

cagggccaca uggagacggu caaguccgca aaacgccucg acgaaggggu gagcguucug     2580

cgagugccuc uaacacaacu gaagcucaaa gaucuggaga agauggucaa cagagagcgg     2640

gaaccaaaac uuuacgaggc cuugaaggcu aggcuggaag cgcauaaaga cgaucccgcc     2700

aaggcauuug cugagccguu cuauaaguac gacaaagccg gaaaucgcac ccaacaggug     2760

aaggccguac guguggaaca aguucagaaa acuggcgucu gggugagaaa ccacaauggu     2820

auagcugaua acgcaaccau ggugcggguc gacguuuuug agaaggccgg gaaauauuac     2880

uuagugccua ucuacucuug gcaaguggcg aagggcauuc ucccagauag ggcuguaguc     2940

gccuaugcag acgaggaagg auggacagug aucgaugagu cauuccgcuu uaaguucguu     3000

cuguacagua augaccuuau uaaagugcag cugaagaaag auagcuuccu aggcuauuuu     3060

uccgggcucg accgagccac cggagcuauc ucucugagag aacaugauuu ggagaagucg     3120

aaaggcaagg acgguaugca ccggauaggg gucaagacug cacugagcuu ccagaaauac     3180

cagaucgacg agaugggaaa ggaaauuagg uuaugccgcc ucaaaaagag acccccugug     3240

agguga                                                                3246


<210>  2708
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2708
augcugaaga gagugcucgg caucgacguc gggauuagcu ccauaggaug ggcccuucug       60

gaguuccagg gugauaucga caacaaagau uugcccucug gcaagauuuu agcuucaggg      120

guuagggugu uuacccaagc agaaaauccu aaaaacggag agagucuggc caagccacgg      180

cgcgacgcgc gacuagcucg uagaaggaca cggcgcagag cccagaggcu cacugauauc      240

aaagaccugu ucuuuaagua caaacuugua acgggcauua gccggaauaa aaucaacucc      300

auauauaaga ccgaagagaa uaaaaaggau ccguggacac ugcgcaaaga agcauuguac      360

cgaaagcucg acggugagga guucgccaga gugcugacuc acaucgcuaa gcauaggggg      420

uuuaaaucua ugcggaaguc ggaagaggaa ggaucagaua ccaaagcaaa ggagacacag      480

cguguccuga ccgagauugg caaaaacaag gacacucuca aaacaggcau cuggcagacc      540

gccggggaaa ugauugcuag ccgccccaag ggagaugcca gaaggaauaa gacggacagu      600

uauaaaaacu ccaucucucg ggaguuacug caagaugaaa cuaagauaau uuucgagaaa      660

cagcgaggua ugaauagcca guuuaccucc gcggaccuug aaaaggagua cucagacauc      720

gcauuccacc aggccccuau ucaaaguaug uugaaaaugg ugggcgggug cacauuugag      780

aaggaugaag agggaaagcg ccugaaaaga gcuccaaaga acagcuauuc ugccgaaguu      840

uucacugcac uaaccaaaau caauaaccuc gugcugaaga caaaaaccgc uaauaucaac      900

gacagguuuc uuacgacuga ggaacggaag aaggcccugg aguuguguca uuccaaugcg      960

aaagucacau ucaagcagcu ccgcggcgag cuggguuuac ccgaagauug gauuuuuaac     1020

accguggacu acgccaaaaa gccuaaaaag acugcaguaa aggagacaga accagaguca     1080

gcuugcuucu uccagaugag aggguauaac gaaauaagga aaagcguugg aaaggaucug     1140

uggaccgaga ucaguaugaa aaaggagauu cucaauucua ucgugacgac ccuugccaca     1200

gaaaaauccg acgaggcuau aaaggcccag cugaaaaaga gcgaaguccc cgaugagauu     1260

gcagagaagg uguugggccu aaacuuucgu ggcuuccugc accucucgau caaagcccug     1320

gacaagauca uuccuuuuau gauggcuggg gauaaauaca cugacgcgug ugauaaggca     1380

ggauacaaag aacaaaucuc ugguauuaag aagauauucc uucggaaacu gaaugacgag     1440

gaaaacgagc agaucaccaa uccaguggcc aagcgagcua uuacacaguu uagaaaaguc     1500

aucaacgcca ucauuaggga auauggcaag uucgaccaaa ugaauauaga guuggcacgc     1560

gaucucgcca aauccuacga cgaucggaag aagaucaaag aggggcagga agacuuuaga     1620

gcugagaagg auagggccgc acagcgcugc aaagacaacg gacuggaucc aaauucaaag     1680

gacaacuuaa guaaauaucg acugugggaa cagcaagaua auggcuucug uauuuacugc     1740

gguaagccca ucgagcuaca gcggcucgcg gaacaggggu auugugaggu ugaccauauu     1800

cugccuuaca gccguucccu ugaugacucu cagcacaaca agguguuggc ucacauccau     1860

gauaaucaac agaaaaagaa ccagauacca uuugagcacu ucggcggaga cggccccaaa     1920

ugggaagagu uuguagggag acugaauagc caguucaaga accucccuaa aucaaagcug     1980

aacaggcuug ugaaacgcaa guuugacgaa aaggauaugg agggauucaa agaacggaau     2040

aucaacgaca cuagauauau uaguagguuc aucaagaauu uugucgagaa ccaucuggag     2100

uucgccgaau ccgcacaaaa acagaaggug uuuguucgaa gcgguucuuu gaccgccuuc     2160

cuccgccacc aguggggccu gauuaaaaag cgggaugagu cagacagaca ccaugcuuua     2220

gaugcccugg ugacagcaug cgcuacccag gccaugguca agaguacugc gaucacggcc     2280

caaaagaugg aaagcuacaa uuggauaaac aaggagaaac caaggaugcc ccuaccgugg     2340

cucaauuuuc gugacgaugu gcagaagucc cuggacgcug uauucguuuc ucgcgcaccu     2400

cggcacaaag ucgggggagc cgcucauaag gagacaauua gaucgaucag gaaguguggc     2460

gaagagaacc uuauuaucga aaagaccccc uugguggaua uagccacuaa gagcgguauc     2520

aaacugaagg acauugagaa acucuacaag aaggaacaga aucgaaaacu guaugagacc     2580

uuaaaggagc ggcuggaaca aaacgggaau gauccaguga aagcauuugg caacccugac     2640

gaucccgucc gcaugccaac aaaugaccuu accaagacug gacccaucau uaaauccauc     2700

aaguugauaa caaacaagaa aucaggccuc aaugugagag cggggauugc cgacaacgga     2760

gagaucgcua ggcuggaugu uuacgaacgg aaggguaaau ucuaucuaau cccuaacuac     2820

guggccgacu uugcaaauaa ggagcugccg aaaaaggcua uuaaagccaa cacgccggaa     2880

accgaguggc ccaucauuga ucgaaauuau aacuucaagu uuagucucuu caaggacgau     2940

cugauaucua ucaaacagaa acuugacagc ccugaggaau uuuacuauua caaaggccca     3000

gauguaucca cugcaucaau uacagucgag agcguggaca gaaagcgcuu gugggaagag     3060

aaaaagaagg ggaaaaucga aaauguuuuc cacaccagga uuggaacaaa gacucugaaa     3120

ucuuuccgua aguuuaacuu agauauccuc ggcaccaaag cccccgugga gauaaagaag     3180

gagacguggg ucggugugau ccggaaaaau gggguagcua acaauaccgg caacaaggcg     3240

auguga                                                                3246


<210>  2709
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2709
auggccgcuu ucaagcccaa cccuaugaau uacaucuuag gccucgacau ugggauagca       60

agcgugggau gggccauggu cgagaucgau aaagaagaga acccaauuag acuuaucgac      120

cuggguguua ggguguuuga acgggcggag guaccgaaga ccggcgauuc cuuggcuaug      180

gcccgccgau uagcacguuc ugugagaagg cugacacggc gcagagccca caggcuacuc      240

cgggcucgcc gacugcuuaa aagagaaggg guccugcagg acgcagauuu cgacgagaau      300

ggauugguga agucacuccc caacacuccu uggcaacuga gggccgcugc ccuggaucgg      360

aaacucacgu gcuuagagug gagugcgguu cugcuucauu ugauuaagca ccguggcuau      420

cugagccagc gcaagaauga aggugagacc gcagacaaag aacuaggggc ccuccugaag      480

ggaguggcug auaacgccca ugcacuucag acaggcgacu uuagaacucc agcugagcug      540

gccuugaaua aauucgagaa ggaauccggc cacaucagga accagcgagg ggauuacucu      600

cauaccuuuu cgcggaaaga ccuccaagcg gagcugaauu uacuguucga aaagcagaag      660

gaguuuucaa acccccacgu cagcgauagu cucaaagaag gaauagagac acuucugaug      720

gcccagcgcc cugcauuguc cggugacgcu gugcagaaga ugcuaggcca cuguaccuuc      780

gagccagccg aacccaaagc ugccaagaau acuuauacag cagagagauu uaucuggcug      840

accaaacuca acaaucugag gauucuugaa caagggucug agcggccucu gacggacacu      900

gaacgcgcca ccuugaugga ugagccauac agaaagagca aacucacaua ugcucaggcg      960

aggaagcugu uaggacugga ggacacugca uucuuuaagg gccuacguua cgguaaagau     1020

aacgccgaag cuccgacccu cauggagaug aaggccuauc augcaaucuc ccgggcccug     1080

gaaaaagagg ggcuuaagga caaaaaguca cccuugaauc ugaguacaga acuccaggau     1140

gagauuggaa ccgcuuucag ccuguuuaag acggacaaag auaucacugg ccgacuuaag     1200

gacagaguac agccugagau acuggaagcc uugcucaaac acauuucuuu cgauaaguuc     1260

guucaaaucu cccugaaagc auuaaggcgc auugugccac ugauggagca gggcaagcgg     1320

uacgacgaag cgugcgcuga gaucuacggg gaucauuauu guaagaaaaa cgccgaggaa     1380

aagaucuacc uacccccuau uccagcagac gagauaagaa accccgucgu gcucagggcc     1440

cugucacagg cucgcaaagu gaucaauugc gucguucgac gguauggaag cccggcccgu     1500

auucacaucg aaacagcaag agaggugggu aagaguuuua aagacaggaa ggaaauagag     1560

aagcgccaag aggaaaaccg gaaagauaga gagaaggcug ccgcgaaauu cagggaauac     1620

uuuccuaauu ucguaggcga gcccaagucu aaagacauuc uuaaguugcg acuguaugaa     1680

cagcagcacg ggaaaugucu cuacuccgga aaggagauca accuggugcg cuuaaaugag     1740

aagggcuaug ucgaaaucga ucaugcccug ccauuuagcc ggaccuggga cgauucguuc     1800

aacaauaaag ugcuuguuuu ggguucugag aaccagaaua aggggaacca aacuccuuac     1860

gaauacuuua auggcaaaga caacuccaga gaguggcagg aguucaaggc uaggguggaa     1920

acaucacguu uuccccgcag uaaaaagcag cggauucucc ugcagaaguu cgaugaggaa     1980

ggauuuaaag agagaaaucu aaacgacacc agguauguca aucgauuccu gugccaauuu     2040

guggcagauc acauccuccu gacgggcaag gggaaacggc gcguauucgc cagcaacgga     2100

cagauuacca accuuuugag agguuucugg ggccugagga agguucgggc ugaaaaugac     2160

cgacaucacg ccuuagaugc aguggucgug gcguguucca cagucgccau gcagcagaaa     2220

auaacuagau uugugcgcua caaggagaug aacgcuuucg acgggaagac caucgauaaa     2280

gagacaggag aaguucucca ucaaaaggcc cacuuuccac agcccuggga guucuuugca     2340

caggaaguga ugauuagggu auucggcaaa ccugacggua agccagaguu ugaagaggcu     2400

gacacuccgg agaaacugcg uacccuucug gccgaaaagu ugucuagccg gcccgaggca     2460

guccacgaau augugacacc ucucuucguu ucaagagccc caaauaggaa gaugaguggg     2520

cagggccaua uggagaccgu gaaauccgcu aagcgccugg augaaggaau cagcguccua     2580

cgagugcccc ugacucaacu caaacugaag ggccuugaga aaaugguaaa cagagagcgg     2640

gaaccuaagu uguacgacgc gcugaaagcc caguuagaga cgcacaagga uaauccagca     2700

aaggcuuuug ccgaacccuu cuauaaauac gacaaggccg ggaacaggac acagcaagug     2760

aaagcuguuc gcaucgagca gguccagaag accggagugu gggugcguaa ucauaacggu     2820

auugcagaua augccacuau ggucagaguu gacguguuug aaaaaggcgg gaaguauuac     2880

cucgugccga uauacucuug gcagguagcg aagggaaucc ugccugaucg ggcugucgug     2940

gccuucaaag acgaggagga uuggaccguu auggacgauu cauuugaguu cagguucgug     3000

cuuuaugcaa acgaccugau uaagcuaaca gccaaaaaga augaauuucu cggcuacuuc     3060

gucagucuga accgcgcuac cggugcaauc gauauucgaa cucacgacac agacagcacg     3120

aaagggaaga auggcaucuu ucaauccgug ggaguuaaga ccgccuuguc uuuccagaaa     3180

aaccagauag augaacuggg caaggagauc agaccaugcc ggcugaaaaa gaggcccccu     3240

gugcgcuga                                                             3249


<210>  2710
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2710
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagaggaaa acccaaucag acuuauugac      120

cuggguguua ggguguuuga gcgggcggaa guaccgaaaa ccggcgauuc cuuggcuaug      180

gcccgccgau uagcacguuc ugugagaagg cugacacggc gcagagccca caggcuacuc      240

cgggcucgcc gacugcuuaa gagagagggg guccugcagg cagccgacuu cgaugagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gggcugccgc gcuggaccgg      360

aagcucacgc cauuagaaug gagugcagug cugcuucauu ugauaaaaca ccguggcuau      420

cugagccagc gcaagaauga gggugaaacc gccgauaagg agcuaggggc ucuccugaaa      480

ggaguugcca acaaugcaca ugcccuucag acaggcgacu uuagaacucc cgcugagcug      540

gcguugaaca aguucgaaaa agaguccggc cacaucagga aucagcgggg agauuacucu      600

cauaccuuuu cgcgaaagga ccuccaagcc gaacugauuu uacuguucga gaaacagaag      660

gaauuuggaa acccucacgu gucagguggc cucaaggagg ggaucgagac acuucugaug      720

acccagcgcc cagcauugag cggagaugcu guccagaaaa ugcuaggcca cugcacuuuc      780

gaacccgccg agccuaaggc ugccaaaaau acauauaccg cagaaagauu uauuuggcug      840

acgaagcuca acaaucugag gauccuugag caagguagug aacggccacu gacugacacc      900

gagcgcgcca cauugaugga ugagccguac agaaaaucca agcucacuua ugcucaggcg      960

aggaaacugu uagggcugga agacaccgca uucuuuaagg gacuacguua cggcaaggac     1020

aacgccgagg cuucuacacu cauggaaaug aaagccuauc augcaauaag ccgggcccug     1080

gagaaggaag gccuuaaaga uaagaaaucc cccuugaauc ugucaaguga gcuccaggac     1140

gagauuggga ccgcuuucag ccuguuuaag acggaugaag acaucacugg acgacuuaag     1200

gauagagugc agccugagau ucuggaagcc uugcucaaac acaucucuuu cgacaaguuc     1260

guacaaaucu cccugaaagc auuaaggcgc auuguuccac ugauggagca ggguaagcgg     1320

uacgaugagg cgugugcuga aauauacggc gaccauuaug ggaaaaagaa cacagaggaa     1380

aagaucuacc uacccccuau uccagccgau gagaucagaa accccguggu ccucagggca     1440

cugucacagg cccgcaaagu gauaaaugga gugguccgac gguauggcag cccggcucgu     1500

auucacaucg aaaccgccag agagguuggu aagaguuuua aagacaggaa ggagaucgaa     1560

aaacgccaag aggaaaaccg gaaggauaga gagaaggcag cugccaaauu cagggaauac     1620

uuuccuaauu ucguggggga gcccaagucu aaagacauuc uuaaguugcg acuguaugag     1680

cagcagcacg gcaaaugccu cuacuccgga aaggaaauca accugguacg cuuaaaugag     1740

aaaggcuaug uggaaauuga ccaugcgcug ccauuuagcc ggacuuggga ugacucguuc     1800

aacaauaagg uccuuguguu ggggucugag aaccagaaua agggaaacca aacaccuuac     1860

gaauacuuua augguaaaga uaacuccaga gaguggcagg aguucaaggc caggguugaa     1920

accucacguu uuccccgcag uaaaaagcag cggauacucc ugcagaaauu cgacgaggau     1980

ggcuuuaagg aauguaaucu aaacgacacg agauauguga auagguuccu gugccaauuu     2040

gucgcugauc acauccuccu gaccgggaag ggaaaacgac ggguguucgc aagcaacggc     2100

cagauuacaa accuuuugcg cgguuucugg gggcugagaa agguaagggc cgagaaugac     2160

cggcaucacg cuuuagaugc cguugugguc gcauguucca cuguggcgau gcagcagaaa     2220

aucacccgau uugucagaua caaggagaug aacgccuucg acggcaaaac aaucgauaag     2280

gaaacuggaa aggugcucca ucagaaaacc cacuuuccac agcccuggga guucuuugcu     2340

caggaaguua ugauucgcgu guucggcaag ccugacggga aaccagaguu ugaagaggcc     2400

gauacaccgg agaagcugag gacccuucug gcagaaaaau ugucuagccg ucccgaggcu     2460

guacacgaau augucacucc ucucuucgug ucacgggccc caaauagaaa gaugagugga     2520

cagggucaua uggagacggu uaaguccgca aaaaggcugg acgaaggcgu gagcguccua     2580

cgcgugcccc ugacacaacu caagcugaaa gaccuugaga agaugguaaa ccgagagaga     2640

gaaccuaaau uguacgaggc ccugaaggcu cgguuagaag cgcacaaaga ugacccagcc     2700

aaggcauuug cugagcccuu cuauaaguac gauaaagccg ggaauaggac ccaacaggug     2760

aaggccguuc gcgucgaaca agugcagaaa acuggagugu ggguccguaa ccauaauggc     2820

auagcugaca acgcaaccau gguuagagug gauguguuug agaaggguga caaauauuac     2880

cucguaccga ucuacucuug gcaggucgcc aaggggauuc ugccugaucg ggcggugguu     2940

cagggcaagg acgaggaaga uuggcaacuu aucgacgauu cauucaauuu uaaauucagu     3000

cugcacccaa acgaccuagu ggaggucauu acaaagaaag cuaggauguu cggauauuuu     3060

gccagcugcc accgcggcac cgggaauauc aacauacgaa uccaugaucu cgaccacaag     3120

auugggaaaa auggcauccu ggaagguauu ggggugaaga cugcauuguc cuuccagaag     3180

uaccagaucg acgagcuggg aaaagagaua agacccuguc gguuaaagaa aaggccuccc     3240

guucgcuga                                                             3249


<210>  2711
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2711
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagacgaga acccaaucug ccuuauugau      120

cuggguguua gaguguuuga aagggcggag guaccgaaaa ccggcgacuc cuuggcuaug      180

gcccggcgcu uagcacgauc ugugcguaga cugacaaggc ggcgcgccca cagacuacuc      240

agggcucggc gccugcuuaa gcgagaaggg guccugcagg cagccgauuu cgacgagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gagcugccgc gcuggauagg      360

aagcucacgc cauuagagug gagugcagug cugcuucauu ugauaaaaca ccggggcuau      420

cugagccagc guaagaauga aggugagacc gccgacaagg aacuaggggc ucuccugaaa      480

ggaguugccg auaacgcaca ugcccuucag acaggcgacu uucgcacucc cgcugagcug      540

gcguugaaua aguucgagaa agaauccggc cacaucagaa accagcgagg ggauuacucu      600

cauaccuuuu cgcggaagga ccuccaagcc gagcugauuu uacuguucga aaaacagaag      660

gaguuuggaa auccucacgu gucagguggc cucaaggaag ggaucgagac acuucugaug      720

acccagcgac cagcauugag cggagaugcu guccagaaaa ugcuaggcca cuguacuuuc      780

gagcccgccg aaccuaaggc ugccaaaaac acauauaccg cagagcgcuu uauuuggcug      840

acgaagcuca auaaccugag aauccuugaa caagguagug agaggccacu gacugacacc      900

gaacgggcca cauugaugga cgagccguac cgcaaaucca agcucacuua ugcucaggcg      960

agaaaacugu uagggcugga ggauaccgca uucuuuaagg gacuaaggua cggcaaggac     1020

aaugccgagg cuucuacacu cauggaaaug aaagccuauc augcaauaag ccgugcccug     1080

gaaaaggagg gccuuaaaga uaagaaaucc cccuugaacc ugucaccuga acuccaggac     1140

gagauuggga ccgcuuucag ucuguuuaag acggaugagg acaucacugg acggcuuaag     1200

gauaggauuc agccagaaau ccuggaggcc uugcucaaac acaucagcuu cgacaaguuc     1260

gugcaaauuu cucugaaagc auuaagaagg auaguacccc ugauggaaca ggguaagcgc     1320

uacgaugagg cgugcgcuga gaucuacggc gaccauuaug ggaaaaagaa uacagaagag     1380

aagauuuacc uaccuccaau ccccgccgau gaaauacgga acccgguugu gcucagagca     1440

cugucccagg ccaggaaagu cauuaacgga guggugcgcc gauauggcuc acccgcucgg     1500

auccacaucg agaccgcccg ugaagucggu aagagcuuua aagacagaaa ggagauugag     1560

aaaaggcaag aagagaaucg caaggaccgg gaaaaggcag cugccaaauu cagagaguac     1620

uuucccaacu ucguugggga accaaagagu aaagauaucc uuaaguugag gcuguaugag     1680

cagcagcacg gcaaaugucu cuacucugga aaggagauua aucugggccg auuaaacgaa     1740

aaaggguaug uggagauaga ccaugcgcug ccuuuuuccc gcacuuggga ugacagcuuc     1800

aauaacaagg uacuuguguu gggaucggaa aaucagaaca aggguaauca aacacccuac     1860

gaguacuuua acggcaaaga uaauucucgg gaauggcagg aguucaaggc cagagucgag     1920

accuccaggu uuccacguag uaaaaagcag cgcauccucc ugcagaaauu cgacgaagau     1980

ggguuuaagg agcggaaccu aaaugacacg agauauguga acagguuccu gugccaauuu     2040

guugcugauc gaaugcggcu caccggaaag ggcaaaaagc gcguguucgc aucaaauggu     2100

cagauuacaa accugcuuag aggguucugg ggcuugagga aaguccgggc cgaaaacgac     2160

cgacaccaug cucuggaugc cgugguaguu gcauguagca cuguggcgau gcagcagaag     2220

aucaccagau uuguccgcua caaagagaug aaugccuucg acggaaagac aaucgauaag     2280

gagacuggcg aaguguuaca ccagaaaacc cauuuucccc agccuuggga guucuuugcu     2340

caggaaguca ugauuagggu guucgggaag ccagacggaa aaccggaguu ugaagaggcc     2400

gacacacccg agaagcuccg uacccugcuu gcagaaaaac uguccucucg gccugaggcu     2460

guucacgaau augugacucc auuguucgua agcagagccc ccaacaggaa gaugucaggu     2520

cagggccaca uggagacggu caagagugca aaacgccucg augaaggggu guccguucug     2580

cgagugccuc uaacacaacu gaagcucaaa gaccuggaga agauggucaa uagagagcgg     2640

gaaccaaaac uuuacgaggc cuugaaggcu aggcuggaag cgcauaaaga ugaccccgcc     2700

aaggcauuug cugagccguu cuauaaguac gauaaagccg gaaaccgcac ccaacaggug     2760

aaggccguac guguggaaca aguucagaaa acuggcgucu gggugagaaa ucacaacggu     2820

auagcugaca augcaaccau ggugcggguc gauguuuuug agaaggggga caaauauuac     2880

uuagugccua ucuacagcug gcaaguggcc aagggcauuc ucccagauag ggcgguaguc     2940

cagggaaagg acgaggaaga uuggcagcug aucgacgauu cuuucaacuu uaaauucuca     3000

cuucauccca augaccuggu ggagguuauu acaaagaaag cucgcauguu cggcuauuuu     3060

gccaguugcc accgagggac cggaaacauc aauauaagaa uccacgaccu agaucauaag     3120

auuggcaaaa acgguauccu cgaagggauu ggagugaaga cugcacugag cuuccagaag     3180

uaccagaucg acgaguuggg cgaggaaaua cggccuugua ggcugaaaaa gcgcccucca     3240

gucagauga                                                             3249


<210>  2712
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2712
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagacgaga acccaaucug ccuuauugau      120

cuggguguua gaguguuuga aagggcggag guaccgaaaa ccggcgacuc cuuggcuaug      180

gcccggcgcu uagcacgauc ugugcguaga cugacaaggc ggcgcgccca cagacuacuc      240

agggcucggc gccugcuuaa gcgagaaggg guccugcagg cagccgauuu cgacgagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gagcugccgc gcuggauagg      360

aagcucacgc cauuagagug gagugcagug cugcuucauu ugauaaaaca ccggggcuau      420

cugagccagc guaagaauga aggugagacc gccgacaagg aacuaggggc ucuccugaaa      480

ggaguugccg auaacgcaca ugcccuucag acaggcgacu uucgcacucc cgcugagcug      540

gcguugaaua aguucgagaa agaauccggc cacaucagaa accagcgagg ggauuacucu      600

cauaccuuuu cgcggaagga ccuccaagcc gagcugauuu uacuguucga aaaacagaag      660

gaguuuggaa auccucacgu gucagguggc cucaaggaag ggaucgagac acuucugaug      720

acccagcgac cagcauugag cggagaugcu guccagaaaa ugcuaggcca cuguacuuuc      780

gagcccgccg aaccuaaggc ugccaaaaac acauauaccg cagagcgcuu uauuuggcug      840

acgaagcuca auaaccugag aauccuugaa caagguagug agaggccacu gacugacacc      900

gaacgggcca cauugaugga cgagccguac cgcaaaucca agcucacuua ugcucaggcg      960

agaaaacugu uagggcugga ggauaccgca uucuuuaagg gacuaaggua cggcaaggac     1020

aaugccgagg cuucuacacu cauggaaaug aaagccuauc augcaauaag ccgugcccug     1080

gaaaaggagg gccuuaaaga uaagaaaucc cccuugaacc ugucaccuga acuccaggac     1140

gagauuggga ccgcuuucag ucuguuuaag acggaugagg acaucacugg acggcuuaag     1200

gauaggauuc agccagaaau ccuggaggcc uugcucaaac acaucagcuu cgacaaguuc     1260

gugcaaauuu cucugaaagc auuaagaagg auaguacccc ugauggaaca ggguaagcgc     1320

uacgaugagg cgugcgcuga gaucuacggc gaccauuaug ggaaaaagaa uacagaagag     1380

aagauuuacc uaccuccaau ccccgccgau gaaauacgga acccgguugu gcucagagca     1440

cugucccagg ccaggaaagu cauuaacgga guggugcgcc gauauggcuc acccgcucgg     1500

auccacaucg agaccgcccg ugaagucggu aagagcuuua aagacagaaa ggagauugag     1560

aaaaggcaag aagagaaucg caaggaccgg gaaaaggcag cugccaaauu cagagaguac     1620

uuucccaacu ucguugggga accaaagagu aaagauaucc uuaaguugag gcuguaugag     1680

cagcagcacg gcaaaugucu cuacucugga aaggagauua aucugggccg auuaaacgaa     1740

aaaggguaug uggagauaga ccaugcgcug ccuuuuuccc gcacuuggga ugacagcuuc     1800

aauaacaagg uacuuguguu gggaucggaa aaucagaaca aggguaauca aacacccuac     1860

gaguacuuua acggcaaaga uaauucucgg gaauggcagg aguucaaggc cagagucgag     1920

accuccaggu uuccacguag uaaaaagcag cgcauccucc ugcagaaauu cgacgaagau     1980

ggguuuaagg agcggaaccu aaaugacacg agauauguga acagguuccu gugccaauuu     2040

guugcugauc gaaugcggcu caccggaaag ggcaaaaagc gcguguucgc aucaaauggu     2100

cagauuacaa accugcuuag aggguucugg ggcuugagga aaguccgggc cgaaaacgac     2160

cgacaccaug cucuggaugc cgugguaguu gcauguagca cuguggcgau gcagcagaag     2220

aucaccagau uuguccgcua caaagagaug aaugccuucg acggaaagac aaucgauaag     2280

gagacuggcg aaguguuaca ccagaaaacc cauuuucccc agccuuggga guucuuugcu     2340

caggaaguca ugauuagggu guucgggaag ccagacggaa aaccggaguu ugaagaggcc     2400

gacacacccg agaagcuccg uacccugcuu gcagaaaaac uguccucucg gccugaggcu     2460

guucacgaau augugacucc auuguucgua agcagagccc ccaacaggaa gaugucaggu     2520

cagggccaca uggagacggu caagagugca aaacgccucg augaaggggu guccguucug     2580

cgagugccuc uaacacaacu gaagcucaaa gaccuggaga agauggucaa uagagagcgg     2640

gaaccaaaac uuuacgaggc cuugaaggcu aggcuggaag cgcauaaaga ugaccccgcc     2700

aaggcauuug cugagccguu cuauaaguac gauaaagccg gaaaccgcac ccaacaggug     2760

aaggccguac guguggaaca aguucagaaa acuggcgucu gggugagaaa ucacaacggu     2820

auagcugaca augcaaccau ggugcggguc gauguuuuug agaaggggga caaauauuac     2880

uuagugccua ucuacagcug gcaaguggcc aagggcauuc ucccagauag ggcgguaguc     2940

cagggaaagg acgaggaaga uuggcagcug aucgacgauu cuuucaacuu uaaauucuca     3000

cuucauccca augaccuggu ggagguuauu acaaagaaag cucgcauguu cggcuauuuu     3060

gccaguugcc accgagggac cggaaacauc aauauaagaa uccacgaccu agaucauaag     3120

auuggcaaaa acgguauccu cgaagggauu ggagugaaga cugcacugag cuuccagaag     3180

uaccagaucg acgaguuggg caaagagaua cggccuugua ggcugaagaa acgcccucca     3240

gucagauga                                                             3249


<210>  2713
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2713
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagacgaga acccaaucug ccuuauugau      120

cuggguguua gaguguuuga aagggcggag guaccgaaaa ccggcgacuc cuuggcugcc      180

gcacggcgcu uagcccgauc ugugcguaga cugacaaggc ggcgcgcuca cagacuacuc      240

agggcacggc gccugcuuaa gcgagaaggg guccugcagg ccgcugauuu cgacgagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gagccgcggc acuggauagg      360

aagcucacgc cauuagagug gagugccgug cugcuucauu ugauaaaaca ccggggcuau      420

cugagccagc guaagaauga aggugagacc gcugacaagg aacuaggggc ccuccugaaa      480

ggaguugcag auaacgcuca ugcccuucag acaggcgacu uucgcacucc cgcggagcug      540

gccuugaaua aguucgagaa agaauccggc cacaucagaa accagcgcgg ggauuacucu      600

cauaccuuuu cgcggaagga ccuccaagca gagcugauuu uacuguucga aaaacagaag      660

gaguuuggaa auccucacgu gucagguggc cucaaggaag ggaucgagac acuucugaug      720

acccagcgac cagcuuugag cggagaugcc guccagaaaa ugcuaggcca cuguacuuuc      780

gagcccgcug aaccuaaggc cgccaaaaac acauauaccg cagagcgcuu uauuuggcug      840

acgaagcuca auaaccugag aauccuugaa caagguagug agaggccacu gacugacacc      900

gaacgggcua cauugaugga cgagccguac cgcaaaucca agcucacuua ugcgcaggca      960

agaaaacugu uagggcugga ggauaccgcc uucuuuaagg gacuaaggua cggcaaggac     1020

aaugcugaag ccucuacacu cauggagaug aaagcauauc augccauaag ccgugcucug     1080

gaaaaggagg gccuuaaaga uaagaaaucc cccuugaacc ugucaccuga acuccaggac     1140

gagauuggga ccgccuucag ucuguuuaag acggaugagg acaucacugg acggcuuaag     1200

gaucgagugc agccagaaau ucuggaggca uugcucaaac acaucagcuu cgacaaguuc     1260

guacaaaucu cucugaaagc guuaagaagg auuguucccc ugauggaaca ggguaagcgc     1320

uacgaugagg cuugcgccga gauauacggc gaccauuaug ggaaaaagaa uacagaagag     1380

aagaucuacc uaccuccaau ucccgcagau gaaauccgga acccgguggu ccucagagcc     1440

cugucccagg cuaggaaagu gauaaacgga gugguccgcc gauauggcag cccugcccgg     1500

auucacaucg agaccgcacg ugaaguuggu aagucauuua aagacagaaa ggagaucgag     1560

aaaaggcaag aagagaaucg caaggaccgg gaaaaggcug ccgcgaaauu cagagaguac     1620

uuucccaacu ucguggggga accaaagagu aaagauauuc uuaaguugag gcuguaugag     1680

cagcagcacg gcaaaugucu cuacucugga aaggagauca aucugggccg auuaaacgaa     1740

aaaggguaug uagagauuga ccaugcccug ccuuuuuccc gcacuuggga ugacagcuuc     1800

aauaacaagg ugcuugucuu gggaucggaa aaucagaaca aggguaauca aacacccuac     1860

gaguacuuua acggcaaaga uaauucucgg gaauggcagg aguucaaggc uagaguggag     1920

accuccaggu uuccacguag uaaaaagcag cgcauacucc ugcagaaauu cgacgaagau     1980

ggguuuaagg agcggaaccu aaaugacacg agauauguua acagguuccu gugccaauuu     2040

guggcagauc gaaugcggcu caccggaaag ggcaaaaagc gcgucuucgc cucaaauggu     2100

cagaucacaa accugcuuag aggguucugg ggcuugagga aagugcgggc ugaaaacgac     2160

cgacaccaug cccuggaugc aguaguugug gcguguagca cugucgccau gcagcagaag     2220

auuaccagau uugugcgcua caaagagaug aaugcuuucg acggaaagac aaucgauaag     2280

gagacuggcg aagucuuaca ccagaaaacc cauuuucccc aaccuuggga guucuuugcc     2340

caggaaguga ugaucagggu uuucgggaag ccagacggaa aaccggaguu ugaagaggca     2400

gacacacccg agaagcuccg uacccugcuu gcugaaaaac uguccucucg gccugaggcc     2460

gugcacgaau auguaacucc auuguucguc agcagagcac ccaacaggaa gaugucaggu     2520

cagggccaca uggagacggu gaagagugcc aaacgccucg augaaggggu uuccgugcug     2580

cgagucccuc uaacacaacu gaagcucaaa gaccuggaga agauggugaa uagagagcgg     2640

gaaccaaaac uuuacgaggc uuugaaggcg aggcuggaag cccauaaaga ugaccccgca     2700

aaggcuuuug ccgagccguu cuauaaguac gauaaagccg gaaaccgcac ccagcaggua     2760

aaggcugugc guguugaaca aguccagaaa acuggcgugu gggugagaaa ucacaacggu     2820

auugcagaca augccaccau gguccggguu gauguguuug agaaggggga caaauauuac     2880

uuagugccua uauacagcug gcagguagcg aagggcaucc ucccagauag ggcugucgug     2940

cagggaaagg acgaggaaga uuggcaacug auugacgauu cuuucaacuu uaaauucuca     3000

cuucauccca augaccuggu ugaggugauc acaaagaaag cccgcauguu cggcuauuuu     3060

gcaaguugcc accgagggac cggaaacauu aauaucagaa uacacgaccu agaucauaag     3120

aucggcaaaa acgguauucu cgaagggauc ggagucaaga cugcccugag cuuccagaag     3180

uaccagauug acgaguuggg caaagagauc cggccuugua ggcugaagaa aaggccccca     3240

gugagauga                                                             3249


<210>  2714
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2714
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagaggaaa acccaaucag acuuauugac      120

cuggguguua ggguguuuga gcgggcggaa guaccgaaaa ccggcgauuc cuuggcuaug      180

gcccgccgau uagcacguuc ugugagaagg cugacacggc gcagagccca caggcuacuc      240

cggacucgcc gacugcuuaa gagagagggg guccugcagg cugcaaauuu cgacgagaac      300

ggauugauca aaucacuccc caauacgcug uggcaacuga gggccgcugc ccucgaucgg      360

aaguuaaccc cucuggaaug gagugcggug cuuuugcauc ugauaaaaca ccguggcuau      420

cuaagccagc gcaagaacga gggugaaaca gcagacaagg agcucggggc ccugcuuaaa      480

ggaguugcug gcaaugccca ugcacugcag acuggcgauu uuagaacccc agcugaguug      540

gcccucaaca aguucgaaaa agaguccggg cacaucagga aucagcgggg agacuacucu      600

cauacauuuu cgcgaaagga ucugcaagcg gaauuaaacc uccucuucga gaaacagaag      660

gaauuuggua auccccacau uucagacggc cuuaaggagg ggaucgagac ccuguugaug      720

acucagcgcc cugcccuaag cggagaugca gugcagaaaa ugcugggcca cugcacauuc      780

gaaccaaccg agcccaaggc ugccaaaaac acguauacua ccgaaagauu uauuuggcuc      840

acaaagcuga auaaccuuag gauccuggag caagguagug aacggccuuu gacugacacc      900

gagcgcgcua cacucaugga ugagccauac agaaaaucca agcugaccua ugcccaggca      960

aggaaauuac uggaacuaga cgacauagcc uucuuuaagg ggcuccguua cggaaaggau     1020

aaugcugagg cgucuacgcu gauggaaaug aaagcauauc augccauuag ccgggcucuu     1080

gagaaggaag gcuugaaaga caagaaaucc ccgcugaacc ucucacccga gcugcaggau     1140

gagaucggca cugccuucag ucuuuuuaag acagacgaag auauuaccgg gcgacugaag     1200

gacagaaucc agccugagau cuuggaagca cuccugaaac acauuaacuu cgauaaguuc     1260

guccaaauca gcuuaaaagc ccugaggcgc auagugccac uaauggagca gggaaagcgg     1320

uacgacgagg cuugugccga aauuuacggu gaucauuaug gcaaaaagaa uacugaggaa     1380

aagaucgacc ugcccccuau accagcagau gagauucaca accccguagu ucugagagcg     1440

cuuucucagg cuaggaaagu gaucaauggg gucgugcgcc gauacggauc cccggcccgg     1500

auccacauug aaacagcacg ugaggugggc aagucauuua aagacagaaa ggagaucgag     1560

aaaaggcaag aagaaaaccg caaggaccgg gagaaggccg cugccaaauu cagagaauau     1620

uuuccuaauu ucgucgguga gcccaagagc aaagauauuu ugaagcugag gcucuacgag     1680

cagcagcaug ggaaaugccu guauaguggc aaggaaauaa acuuaggacg acugaaugag     1740

aaaggcuacg uugaaaucga ccacgcacuu ccauuuucuc gcaccuggga ugacuccuuc     1800

aacaauaagg uguugguacu cgggagcgag aaccagaaua agggaaacca aacgccuuau     1860

gaauacuuua augguaaaga uaacucgcgg gaguggcagg aguucaaggc uagaguggaa     1920

accucuaggu uuccccguuc caaaaagcag cgcauucugc uacagaaauu cgacgaggau     1980

ggcuuuaagg aacggaaucu gaacgacaca agauacguca acagguuccu cugucaauuu     2040

guggccgauc gaaugcggcu gacugggaag ggcaaaaagc gcguuuucgc gucaaaugga     2100

cagaucacca accuuuugag agguuucugg gggcugagga aagugcgggc cgagaaugac     2160

cgacaucacg cuuuagaugc agucguggua gccugcagua caguugcuau gcagcagaag     2220

aucacuagau uugugcgcua uaaagagaug aacgccuucg acggcaagac cauugauaag     2280

gaaacaggag agguccucca ucagaaaacc cacuuuccac agcccuggga guucuuugca     2340

caggaaguga ugauaagggu cuucggcaag ccugacggga aaccagaauu ugaggaggcg     2400

gacacuccgg aaaagcugcg uacgcuucug acagagaaau ugagcucccg gcccgaagcc     2460

gugcacgagu acguuacccc ucucuucgug ucuagagcuc caaauaggaa gaugagcgga     2520

cagggucaua uggaaacugu aaagucagcc aaacgccugg augagggcau caguguccua     2580

cgagugcccc ugacccaacu caagcugaaa gaccuugaga agaugguuaa cagagaacgg     2640

gagccuaaau uguaugaagc acugaaggcu agguuagagg cccacaaaga ugacccagca     2700

aaggccuuug cugaacccuu cuacaaguau gauaaagcgg ggaaucgcac acaacaggug     2760

aaggccaugc gugucgagca agugcagaaa accggaguau gggugagaaa ccauaauggc     2820

auugcagaca acgcuacuau gguucggguc gauguguuug agaaggguga caaauacuau     2880

cucgugccga ucuacuccug gcaggucgcc aaggggauuc ugccugauag ggccguugug     2940

cagggcaagg acgaagagga uuggcaacuu aucgacgaua gcuucaauuu uaaauucucu     3000

cugcacccaa acgaccuagu ggaaguaaua acaaagaaag cucgcauguu cggauacuuu     3060

gcaucauguc accgaggcac ggggaauauc aacauuagaa uccaugaccu cgaucacaag     3120

auuggcaaaa auggaauccu ggaggguaua ggggucaaga ccgccuugag uuuccagaag     3180

uaucagauug acgagcuggg aaaagaaauc cggcccugca gguuaaagaa acgcccuccc     3240

gugagauga                                                             3249


<210>  2715
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2715
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagacgaga acccaaucug ccuuauugau      120

cuggguguua gaguguuuga aagggcggag guaccgaaaa ccggcgacuc cuuggcuaug      180

gcccggcgcu uagcacgauc ugugcguaga cugacaaggc ggcgcgccca cagacuacuc      240

agggcucggc gccugcuuaa gcgagaaggg guccugcagg cagccgauuu cgacgagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gagcugccgc gcuggauagg      360

aagcucacgc cauuagagug gagugcagug cugcuucauu ugauaaaaca ccggggcuau      420

cugagccagc guaagaauga aggugagacc gccgacaagg aacuaggggc ucuccugaaa      480

ggaguugccg auaacgcaca ugcuuuacag acaggcgacu uucgcacucc cgccgagcug      540

gcguugaaua aguucgagaa agaauccggc cacaucagaa accagagggg agauuacucu      600

cauaccuuuu cgcggaagga ccuccaagcc gagcugaauu uacuguucga aaaacagaag      660

gaguuuggga acccucacau uucagauggu cucaaggaag gcaucgagac acuucugaug      720

acccagcgac cagcauugag cggggacgcu gugcagaaaa ugcuaggaca cuguacuuuc      780

gagcccgccg aaccuaaggc ugccaaaaau acauauaccg cagagcgcuu uauuuggcug      840

acgaagcuca acaaucugag aauccuugaa caaggcagug agaggccacu gacugacacc      900

gaacggacaa cuuugauaga ugagccguac cgcaaaucca agcucaccua ugcccaggcu      960

agaaaacugu uagagcugga caacacagcg uucuuuaagg gucuaaggua cgggaaggau     1020

aaugcagaag ccucuacccu cauggagaug aaggcuuauc augccauuag ccgugcacug     1080

gaaaaagagg gacuuaaaga caagaaaucc cccuugaacc ugucaccuga acuccaggau     1140

gagaucggca cggccuucag ucuguuuaag acugacgagg auauuacagg ccggcuuaag     1200

gaccgagucc agccagaaau ccuggaggcu uugcucaaac acaucagcuu cgauaaguuc     1260

gugcaaauuu cucugaaagc cuuaagaagg auaguacccc ugauggaaca ggggaagcgc     1320

uacgacgagg caugcgcgga gaucuacgga gaucauuaug guaaaaagaa caccgaagag     1380

aagauugacc uaccuccaau ccccgcugac gaaauacgga auccgguugu gcucagagcc     1440

cugucccagg caaggaaagu cauuaacggc guggugcgcc gauacgggag cccugcccgg     1500

auccacaucg agacugcucg ugaagucgga aagucauuua aagauagaaa ggagauugag     1560

aaaaggcaag aagagaaucg caaggaccgg gaaaaggccg caacaaaauu cagagaguau     1620

uuucccaacu ucguuggcga accaaagagu aaagauaucc uuaaguugag gcuguacgag     1680

cagcagcacg guaaaugucu cuauucuggg aaggagauua aucugggccg auuaaacgaa     1740

aaaggauacg uggagauaga ccaugcucug ccuuuuuccc gcaccuggga ugacagcuuc     1800

aauaacaagg uacuuguguu gggcucggaa aaucagaaca aggggaauca aacgcccuau     1860

gaguacuuua acggaaaaga uaauucucgg gaauggcagg aguucaaggc cagagucgag     1920

accuccaggu uuccacguag uaaaaagcag cgcauccucc ugcagaaauu cgacgaagag     1980

gguuuuaagg aacggaaccu aaaugauaca agauacguga acagguuccu gugccaauuu     2040

guugcggacc acaugcuccu gacuggcaag gggaaacgac ggguguucgc cucaaacgga     2100

cagauuacca aucuuuugcg cggcuucugg ggucugagaa aggucagggc ugagaacgau     2160

cggcaucacg cauuagacgc cgugguaguu gcuuguagca caguggccau gcagcagaaa     2220

aucacucgau uugucagaua uaaggagaug aaugcauucg augggaaaac caucgacaag     2280

gaaacaggcg aggugcucca ucaaaagacc cacuuucccc agccuuggga guucuuugcg     2340

caggaaguca ugauucgcgu guucggaaaa ccagacggca agccggaauu ugaggaggcc     2400

gauacucccg aaaaacugag gacgcuucug gcugagaagu uguccucucg uccugaagcc     2460

guucacgagu acgugacacc acucuucgua agccgggcac ccaacagaaa aaugucaggg     2520

cagggacaua uggaaaccgu caagagugcu aagaggcugg acgagggugu guccguucua     2580

cgcgugccuc ugacucaacu caaacugaag gaucuugaga aaauggucaa ucgagaaaga     2640

gagccaaagu uguaugaagc ccugaaagca cgguuagagg cccacaagga cgaucccgcu     2700

aaagcguuug ccgaaccguu cuacaaguau gacaaggcag gcaacaggac ccaacaggug     2760

aaagcuguac gcguggagca gguucagaag acaggggucu gggugcguaa ucauaacgga     2820

auagccgaua augccaccau ggugagaguc gacguuuuug agaaaggcga uaaguacuau     2880

cucgugccua ucuacagcug gcaaguggcu aaagguauuc ugccagaccg ggcaguaguc     2940

caggggaagg augaagagga cuggcagcuu aucgaugacu cuuucaacuu uaaguucuca     3000

cugcacccca augaccuagu ggaaguuauu acuaaaaagg ccaggauguu cggcuacuuu     3060

gcgaguugcc accgcggaac aggcaacauc aauauacgaa uccaugaucu cgaccacaaa     3120

auugggaaga acggaauccu ggagggcauu ggugugaaaa cggcuuugag cuuccagaag     3180

uaucagaucg augagcuggg gaaggaaaua agaccuuguc ggcuuaaaaa gaggccccca     3240

guccgcuga                                                             3249


<210>  2716
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2716
auggccgcuu ucaagcccaa cccuaugaau uacauccugg gccucgacau ugggauagca       60

agcgugggau gggccaucgu cgagauugau gaagaggaaa acccaaucag acuuauugac      120

cuggguguua ggguguuuga gcgggcggaa guaccgaaaa ccggcgauuc cuuggcugcc      180

gcacgccgau uagcccguuc ugugagaagg cugacacggc gcagagcuca caggcuacuc      240

cgggcacgcc gacugcuuaa gagagagggg guccugcagg ccgcugacuu cgaugagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gggccgcggc acuggaccgg      360

aagcucacgc cauuagaaug gagugccgug cugcuucauu ugauaaaaca ccguggcuau      420

cugagccagc gcaagaauga gggugaaacc gcugauaagg agcuaggggc ccuccugaaa      480

ggaguugcag acaacacaca ugcccuucag acuggcgauu uuagaacccc cgcugagcug      540

gcguugaaua aguucgaaaa agaguccggc cacaucagga accagcgagg ggacuacucu      600

cauacauuua aucggaagga ucuccaagcc gaacugaacu uacuguucga gaaacagaag      660

gaauuuggaa auccucacgu gucggacggu cucaaggagg gcauugagac ccuucugaug      720

acucagcgcc cagcauuguc aggggaugcu guccagaaaa ugcuaggaca cugcacauuc      780

gaacccaccg agccuaaggc cgcuaaaaac acguauacug ccgaaagauu uguguggcug      840

accaagcuca auaaccugag gauccuugag caaggcagcg aacggccacu gacagacacu      900

gagcgcgcaa ccuugaugga cgagccguac agaaaaagua agcucacaua ugcccaggcu      960

aggaaacugu uagaucugga cgauaccgcg uucuuuaagg gucuacguua cgggaaggac     1020

aaugcagaag ccuccacgcu cauggagaug aaggcuuauc augccauuuc ucgggcacug     1080

gaaaaagagg gacuuaaaga uaagaaaagc cccuugaacc uguccccuga acuccaggac     1140

gagaucggca cugccuucuc acuguuuaag acagaugagg acauaaccgg ccgacuuaag     1200

gauagaguac agccagaaau ucuggaggcu uugcucaaac acaucaguuu cgacaaguuc     1260

guucaaauua gccugaaagc cuuaaggcgc aucgugcccc ugauggaaca ggggaaccgg     1320

uacgaugagg cauguacuga gaucuacgga gaccauuaug guaagaaaaa uacagaagag     1380

aagauuuacc uaccuccaau acccgcggac gaaaucagaa acccggucgu gcucagggcu     1440

cugucucagg cccgcaaggu gauuaauggc gucguucgac gguauggguc cccugcacgu     1500

auccacauag agaccgccag agaaguggga aaaucauuua aggauaggaa agagauugag     1560

aagcgccaag aagagaaccg gaaagacaga gaaaagagcg cugccaaguu cagggaguac     1620

uuucccaauu ucguaggcga accaaaaagu aaggauaucc uuaaauugcg acuguaugag     1680

cagcagcacg guaagugccu cuacucuggg aaagagauca accugggccg cuuaaaugaa     1740

aagggauaug uggagauuga ccaugcacug ccuuuuuccc ggacguggga ugacagcuuc     1800

aacaauaaag uccuugcuuu gggcucggaa aaccagaaua aggggaacca aacccccuac     1860

gaguacuuua auggaaagga uaacucuaga gaauggcagg aguucaaagc caggguggag     1920

acaucccguu uuccacgcuc aaagaaacag cggauccucc ugcagaaguu cgacgaagau     1980

gguuuuaaag agagaaaucu aaacgacacu agguauauua accgauuccu gugucaauuu     2040

guugcggauc acaugcuccu gaccggcaag gggaagcggc gcguguucgc caguaaugga     2100

cagauaacaa accuuuugag aggcuucugg ggucugagga aaguccgggc ugaaaaugac     2160

cgacaucacg cauuagaugc cgugguaguu gcuugcagca cuaucgccau gcagcagaag     2220

auuaccagau uugugcgcua caaagagaug aacgcauucg acgggaagac aaucgacaaa     2280

gagaccggcg aaguccucca ucaaaaggcg cacuuucccc agccuuggga guucuuugcc     2340

caggaaguga ugaucagggu cuucggaaag ccagauggca aaccggaguu ugaagaggcu     2400

gacacucccg agaagcugcg uacgcuucug gccgaaaaau uguccucucg gccugaggca     2460

gugcacaagu auguuacacc acucuucauu agcagagcuc ccaauaggaa aaugucaggg     2520

cagggacaua uggaaaccgu gaagagugcc aagcgccugg augaggguau auccguacua     2580

cgagucccuc ugacucaacu caaacugaag gaccuugaga aaauggugaa cagagaacgg     2640

gagccaaagu uguacgaagc acugaaagcc agguuagagg cucacaagga ugaccccgcg     2700

aaagccuuug cagaaccguu cuauaaguac gauaaggcug gcaaucgcac ccagcagguu     2760

aaagccgugc gugucgagca agugcagaag acagggguau gggugcauaa ccacaaugga     2820

aucgccgaca acgcuaccau uguuagaguc gauguguuug aaaaaggcgg uaaguauuac     2880

cucgugccua ucuacagcug gcaggucgca aaagggauuc ugccagaccg ggccguugug     2940

cagggcaagg augaggagga cuggacugug auggaugacu cuuucgaauu uaaguucgua     3000

cuuuaugcga augaccugau caaacuaaca gcuaagaaaa acgaguuccu cggauacuuu     3060

gucucacuga auagggccac gggcgcaaua gauauccgca cccacgacac ugauaguaca     3120

aaggggaaaa acggaauuuu ccaaagcgug ggcguuaaga ccgccuuguc cuuucagaag     3180

uaucagaucg acgaacuggg uaaagagauu cgacccugua gauuaaagaa acggccuccc     3240

gugagguga                                                             3249


<210>  2717
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2717
auggccgcuu ucaagcccaa cagcaucaau uacauucugg gccucgacau agggaucgca       60

uccgugggau gggccauggu cgagauugau aaagaagaga acccuaucag acuuauugac      120

cuggguguua ggguguuuga acgggcggag guaccaaaga ccggcgauuc uuuggcugcc      180

gcacgccgau uagcccguuc agugagaagg cugacacggc gcagagcuca caggcuacuc      240

cggacucgcc gacugcuuaa aagagaaggg guccugcagg cagccaauuu cgacaagaac      300

ggauugauca aaagucuccc gaauacgcug uggcaacuga gggcugccgc gcucgaucgg      360

aaguuaacuc cccuggagug gagcgcagug cuuuugcauc ugauaaagca ccguggcuau      420

cuaucccagc gcaaaaacga gggugaaaca gccgacaagg agcucggggc ucugcuuaaa      480

ggaguugccg gcaaugcaca ugcucugcag acuggcgauu uuagaacccc ugccgaauug      540

gcgcucaaca aguucgagaa agagucuggg cacaucagga aucagcgggg agacuacucg      600

cauacauuuu cacgaaagga ucugcaagcc gaauuaaacc uccuguucga gaagcagaaa      660

gaauuuggua auccacacau uagcggcggg cuuaaggagg gaaucgaaac ccuguugaug      720

acucagcgcc ccgcacuaag uggcgacgcu gugcagaaaa ugcuggguca cugcacauuc      780

gagccuaccg agccaaaggc cgcuaaaaac acguauacua ccgaaagauu uauuuggcuc      840

acaaagcuga auaaccuuag gauccuggag caaggguccg aacggcccuu gacugauacc      900

gagcgcgcca cacucaugga cgaaccuuac agaaaaucua agcugaccua ugcacaggcc      960

aggaaguuac ugagccuaga ggauacggcu uucuuuaaag gacuccguua cggcaaggac     1020

aaugcggagg cauccacucu gauggaaaug aaagccuauc auacaauauc acgggcucuu     1080

gagaaggaag gcuugaaaga caagaagagu ccacugaacc ucagcccgga gcugcaggau     1140

gaaauuggga ccgccuucuc ucuuuuuaaa acugacgagg auaucacagg acgacugaag     1200

gacaggauuc agcccgagau cuuggaagca cuccugaaac acaucuccuu cgauaaguuc     1260

guccaaauuu cauuaaaagc ccugagacgc auagugccuc uaauggagca ggguaagcgg     1320

uacgacgagg cuugugccga aaucuacggc gaucauuaug ggaagaaaaa caccgaggaa     1380

aagauuuacc ucccacccau cccugcagac gagauaagaa auccaguagu ucugagggcg     1440

cuuagccagg cucgcaaagu gauuaacgga gucgugcgac gguauggcag ucccgcccgu     1500

auccacaucg aaacggcaag agaggugggu aagucuuuua aagauaggaa ggaaauugag     1560

aagcgccaag aggaaaaucg gaaagacaga gagaaggccg cugccaaauu cagggaauac     1620

uuuccgaacu ucgucgggga gccuaagucc aaagauaucu ugaagcugcg acucuaugaa     1680

cagcagcacg gcaaaugccu guacagcgga aaggagauua auuuaguucg ccugaacgag     1740

aagggcuaug uggaaauaga ccaugcacuu cccuuuucgc ggaccuggga cgauucuuuc     1800

aauaacaaag uauuggugcu cggguccgag aaucagaaca agggaaauca aacaccauac     1860

gaauacuuua acgguaaaga caauucaaga gaguggcagg aguucaaggc uagggucgaa     1920

accagucguu uuccucgcag caaaaagcag cggauccugc uacagaaguu cgaugaggac     1980

ggcuuuaaag aaagaaaccu gaaugauacc agguauguga accgauuccu cugucaauuu     2040

guugccgacc acauucugcu uacagggaag ggaaaacggc gcguguucgc guccaacggc     2100

cagaucacua auuugcugag agguuucugg ggguuaagga agguccgggc cgagaacgau     2160

cgacaucacg cucucgacgc agugguaguu gccugcucua ccguggcuau gcagcagaaa     2220

aucacaagau uuguccgcua caaggaaaug aaugccuucg auggcaagac cauugacaaa     2280

gagacuggag aggugcugca ucaaaagacg cacuuucccc agccauggga guucuuugca     2340

caggaaguca ugauaagggu guucggcaaa cccgauggga agccugaauu ugaggaagcg     2400

gacacacuug agaaacugcg uaccuugcuc gccgagaagc ugagcucacg gccagaagcu     2460

guucacgagu augugacucc gcuauucgua aguagagccc ccaacaggaa gauguccgga     2520

cagggucaua uggaaaccgu caaaagcgca aagcgccugg augagggcgu gucuguucuc     2580

cgagugccuc ugacacaacu uaaauugaag gaccuggaga aaauggucaa uagagaacgg     2640

gagccaaagu uauacgaagc ucucaaagcc aggcuggagg cacacaagga cgaucccgcc     2700

aaggcuuuug cggaaccuuu cuauaaauac gacaaggccg ggaaccgcac ccaacaggug     2760

aaagcaguac guguggagca gguucagaag acuggagucu gggugagaaa ucauaacggc     2820

aucgcugaua augccacaau ggugcggguc gacguuuuug aaaaagguga uaaguauuac     2880

cuugugccaa uuuacucaug gcaaguggcc aaggggaucc ugcccgacag ggcuguaguc     2940

cagggcaaag augaggagga cuggcagcua auugaugaca guuucaacuu uaaguucagc     3000

cuccacccga augaucuggu ggaaguuauc acgaaaaagg cacgcauguu cggauauuuu     3060

gccuccuguc accgaggcac cgggaacaua aauauccgga uucaugacuu ggaucacaaa     3120

aucggaaaga acggcauucu ggaggguauc ggggugaaga cugcguuauc uuuccagaaa     3180

uaccagauag acgaacucgg aaaggagauu agaccuugca ggcugaaaaa gcgcccaccc     3240

gucagauga                                                             3249


<210>  2718
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2718
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau agggaucgca       60

agcgugggau gggccauggu cgagauugau gaagaggaaa acccaaucag acuuauugac      120

cuggguguua ggguguuuga gcgggcggaa guaccgaaaa ccggcgauuc cuuggcuaug      180

gugcgccgau uagcccguuc ugucagaagg cugacacggc gcagagcaca caggcuacuc      240

cgggcccgcc gacugcuuaa gagagagggg gugcugcagg cugcagacuu cgaugagaau      300

ggauugauca aaucacuccc caacacuccu uggcaacuga gggccgcugc ccuggaccgg      360

aagcucacgc cauuagaaug gagugcgguu cugcuucauu ugauaaaaca ccguggcuau      420

cugagccagc gcaagaauga gggugaaacc gcagauaagg agcuaggggc ccuccugaaa      480

ggaguggcug acaacgccca ugcacuucag acaggcgauu uuagaacucc cgcugagcug      540

gccuugaaua aguucgaaaa agaguccggc cacaucagga accagcgagg ggacuacucu      600

cauaccuuuu cgcggaagga ucuccaagcg gaacugauuu uacuguucga gaaacagaag      660

gaauuuggaa auccucacau cucagguggc cucaaggagg ggauugagac acuucugaug      720

acccagcgcc cagccuugag cggagacgca guccagaaaa ugcuaggcca cugcacuuuc      780

gaacccgcug agccuaaggc cgcuaaaaac acauauaccg ccgaaagauu uaucuggcug      840

acgaagcuca auaaccugag gauacuugag caagguagug aacggccacu gacugauacc      900

gagcgcgcaa cauugaugga cgagccguac agaaaaucca agcucacuua ugcccaggcu      960

aggaaacugu uagggcugga agacaccgcg uucuuuaagg gacuacguua cggcaaggau     1020

aaugcagagg ccucuacacu cauggaaaug aaggcuuauc augccauuag ccgggcacug     1080

gagaaagaag gccuuaaaga caagaaaucc cccuugaacc ugucaccuga gcuccaggau     1140

gagaucggga ccgccuucag ucuguuuaag acggacgaag auauuacugg acgacuuaag     1200

gacagaaucc agccagagau ccuggaggcu uugcucaaac acauuagcuu cgauaaguuc     1260

gugcaaauau cucugaaagc cuuaaggcgc aucguacccc ugauggaaca ggguaagcgg     1320

uacgacgagg caugugcgga aauuuacggc gaucauuaug ggaaaaagaa uacagaggaa     1380

aagaucuacc uaccuccaau acccgcugac gagauuagaa acccgguugu gcucagggcc     1440

cugucccagg cacgcaaagu caucaacgga guggugcgac gguauggcag cccugcccgu     1500

auccacauug aaaccgcuag agaggucggu aagucauuua aagauaggaa ggagaucgaa     1560

aaacgccaag aggaaaaucg gaaggacaga gagaaggccg cagcuaaauu cagggaauac     1620

uuucccaacu ucguugggga gccaaagagu aaagacauuc uuaaguugcg acuguaugag     1680

cagcagcacg gcaaaugccu cuacucugga aaggaaauaa aucugggccg cuuaaacgag     1740

aaaggguaug uggaaaucga ucaugcccug ccuuuuuccc ggacuuggga cgauagcuuc     1800

aauaacaagg uacuuguguu gggaucggag aaucagaaca aggguaauca aacacccuac     1860

gaauacuuua acggcaaaga caauucuaga gaguggcagg aguucaaggc gagggucgaa     1920

accucccguu uuccacgcag uaaaaagcag cggauucucc ugcagaaauu cgaugaggac     1980

ggguuuaagg aaagaaaccu aaaugauacg agguauguga accgauuccu gugucaauuu     2040

guugccgacc ggaugcgccu caccggaaag ggcaaaaaga gaguguucgc uucaaauggu     2100

cagaucacaa accugcuuag gggguucugg ggcuugcgga aaguccgagc agagaacgau     2160

agacaccaug cccuggacgc ugugguaguu gccugcagca cuguggcaau gcagcagaag     2220

aucacccgcu uugucaggua caaagagaug aaugcguucg auggaaagac aauugacaag     2280

gaaacuggcg agguguuaca ccagaaaacc cauuuucccc agccuuggga guucuuugcc     2340

caggaaguca ugauacgugu guucgggaag ccagauggaa aaccggaauu ugaggaggcu     2400

gacacacccg aaaagcuccg gacccugcuu gccgagaaac uguccucuag accugaagca     2460

guucacgagu augugacucc auuguucgua agcagggcuc ccaaccgcaa gaugucaggu     2520

cagggccaca uggaaacggu caagagugcc aaacgacucg acgagggggu guccguucug     2580

agagugccuc uaacacaacu gaagcucaaa gaucuggaga agauggucaa ucgggaaagg     2640

gagccaaaac uuuacgaagc auugaaggcc cgccuggagg cucauaaaga cgaucccgcg     2700

aaggccuuug cagaaccguu cuauaaguac gacaaagcug gaaaccguac ccaacaggug     2760

aaggccguaa gaguggagca aguucagaaa acuggcgucu gggugcggaa ucacaacggu     2820

aucgccgaua augcuaccau ggugaggguc gacguuuuug agaaggggga uaaauauuac     2880

uuagugccua uuuacagcug gcaaguggca aagggcaucc ucccagaccg cgccguaguc     2940

cagggaaagg augaagagga cuggcagcug auugaugacu cuuucaacuu uaaauucuca     3000

cuucauccca augaucuggu ggaaguuauc acaaagaaag cgcgaauguu cggcuauuuu     3060

gcuaguuguc acagagggac cggaaacaua aauauccgga uucacgaccu agaccauaag     3120

aucggcaaaa acgguauucu cgaggggauc ggagugaaga cugcccugag cuuccagaag     3180

uaccagauag augaguuggg caaagaaauu aggccuugcc gccugaagaa aagaccccca     3240

gucagguga                                                             3249


<210>  2719
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2719
augagagugc ugggccucga cgccgggauc gcuagccuug gaugggcacu gauugagaua       60

gaagagucca acagggguga auugucucag ggcaccauca uuggggccgg aacauggaug      120

uucgaugcgc ccgaggaaaa gacucaagcu ggcgccaaau uaaagucaga gcagcggcgc      180

acguuucgag gucagcguag agucguuagg cggcgcagac agaggaugaa ugaggugcgg      240

cgcauccugc acagucaugg gcuacucccu agcuccgacc gagaugcacu gaaacaacca      300

ggacuugacc cguggagaau uagggccgaa gcucuggauc gguugcucgg ccccguagag      360

cuggcagugg cccugggcca caucgcucgu caucgcgggu ucaagucuaa cucgaaagga      420

gccaagacca augacccugc ggaugacaca ucaaagauga aaagagcagu caacgaaacu      480

agggagaagc ucgcccgguu ugguagcgcu gccaaaaugu uaguggagga ugaaaguuuc      540

guucugcgac agaccccaac aaagaauggc gcauccgaga uagugcgcag auuuaggaac      600

cgggaagggg acuacucucg cagccuuuug agagaugacc uggcugccga gaugagggcg      660

cuauucaccg cccaggcacg uuuucagucc gcuaucgcca cugcugaucu ccaaacagcc      720

uucaccaaag cagccuuuuu ccagcggccc cugcaggacu cagaaaaacu ugucggaccu      780

ugcccauuug agguggacga gaagcgagcu cccaagagag gcuauaguuu cgaacuguuu      840

agguucuuga gccgccucaa ucacguaacg cugcgggaug guaagcagga gagaacuuua      900

accagggacg aacuggcgcu cgcagccgcu gauuucgggg ccgcagccaa aguuucuuuu      960

acagcccuuc gcaagaaacu gaaguugccu gagacuaccg uguucgucgg agugaaagcu     1020

gacgaagagu ccaagcuaga uguggucgca cgaucaggca aggcggcuga gggcacagcc     1080

cggcugcgua gcguuauugu ggacgcacuc ggggaacugg ccuggggagc ccuucugugu     1140

aguccagaga aauuggauaa gaucgcugaa guaauuucuu uuagauccga caucgguagg     1200

auaagcgagg gccucgcaca agcugggugc aacgccccgc ugguggaugc guuaaccgcc     1260

gcugcaucgg acggacgcuu cgaucccuuu acgggcgccg gucauauuuc uuccaaagcu     1320

gcccggaaua uccugucagg gcuaagacag ggcaugacuu acgacaaggc auguugcgcg     1380

gccgacuaug aucacacagc uaguagggaa cgaggagccu ucgacgucgg cgggcacgga     1440

cgcgaggcac ucaaacggau ucugcaggag gaaagaauca gcagggagcu uguggguucc     1500

ccuaccgcuc guaaggccuu gaucgaaucu auuaagcaag uuaaagcaau aguggagcgc     1560

uacggcgucc cagaucggau ccauguggag cuggccagag acguaggaaa gagcauugaa     1620

gagagggaag agaucacucg agggauagaa aaacggaacc gccagaagga uaaacucaga     1680

ggccuguuug agaaggaggu ugguaggccc ccucaggacg gggcucgggg caaggaagaa     1740

cuucugcgau ucgaauugug gucagagcag augggaagau gucucuauac agaugacuac     1800

auuaguccau cccaacuggu ggcgaccgau gacgccgucc agguggauca caucuuaccc     1860

uggagccgcu uugcagacga uucuuaugcu aauaaaacgc ugugcauggc caaggccaac     1920

caggacaaaa agggcaggac cccguacgaa ugguucaaag cugagaagac agacacugag     1980

ugggaugcau uuaucguccg uguggaagcc cuagcggaca ugaagggguu caaaaagcgg     2040

aauuacaaac ucagaaacgc ugaggaagcc gcagccaagu uuaggaaucg caaccugaac     2100

gauacccgau gggcuuguag acuuuuggca gaggcccuga aacagcucua uccuaaggga     2160

gaaaaagaca aggaugguaa ggagcggagg cgcguuuucu cacgucccgg cgcgcugaca     2220

gacagauuac ggagggcuug ggggcugcaa uggaugaaaa agaguacuaa aggagaucgc     2280

auuccagacg aucgacauca cgcccuugac gcaaucguga uugccgcuac cacagagagc     2340

uugcuccaga gagccacccg ggaaguacag gagauagaag auaagggccu gcacuacgac     2400

cuagucaaaa augugacucc ucccuggcca gguuucaggg agcaggcagu ugaggcugug     2460

gaaaaggucu uuguggcccg cgcggagaga aggcgggccc gugggaaggc acaugaugcu     2520

acgauccgcc acauugccgu acgagaaggc gagcaaagag uguaugaaag gcggaaaguu     2580

gcugagcuga agcucgcaga ccuggaccgc gucaaagaug ccgagagaaa cgccaggcuu     2640

aucgaaaagu ugcggaauug gaucgaggcu ggauccccca aagacgaucc uccacugucu     2700

ccgaagggcg accccauuuu caaggugcga uuagugacaa aaucgaaggu caacauagcg     2760

cucgauaccg ggaauccuaa aagaccagga acuguugacc gcggcgaaau ggcaagggug     2820

gauguguuuc ggaaggccag caaaaagggu aaguacgagu auuaccuggu acccaucuau     2880

ccucaugaca uugcuaccau gaaaacacca cccauccgug ccguccaggc auacaagccg     2940

gaagaugagu ggccugagau ggacuccuca uacgaguucu gcuggagucu ugugccaaug     3000

accuaucugc agguuauuuc uagcaaaggg gagaucuuug aaggauacua uagaggcaug     3060

aacagguccg ugggugccau acaguuguca gcucacagca auucuuccga ugucgugcaa     3120

ggcaucgggg cgcgcacucu cacagaguuc aagaaauuua acguugaccg guucggacga     3180

aagcaugaag uggagagaga gcugaggacg uggcgcggcg aaaccuggcg ggggaaagcc     3240

uacauuuga                                                             3249


<210>  2720
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2720
augaagaaca cccuguacgg caucgggcuc gacauuggag uggccagcgu cgguugggcu       60

guugugggcc uuaaugggac aggagagccc guaggccugc acagauuggg ugugaggaua      120

uucgauaaag cagaacagcc uaagacuggg gaguccuuag ccgcgccacg gcgcauggcu      180

cgaggaaugc guagaaggcu gcggcgcaaa gcccuaagaa gggcagacgu cuaugcccuc      240

cuggaacggu cuggccuuuc aacgcgcgag gcucuggcac auauguuuga agccggcggg      300

uuggaggaua ucuacgcucu ccgaaccaga gcccuggacg agccgguggg aaaggcggag      360

uucaguagga uucugcucca cuuagcacaa cggcgugguu uuaaaagcaa ccgcagaaca      420

gccuccgaug gcgaagacgg gaggcugcuu gcugccguua augaaaaccg gcgacgcaug      480

gcacagggag gcuggagaac ugugggugag auguuguaua ggcaugaggc uuucgcccug      540

cacaagcgga auaaggcgga ugaauaccua ucuaccgucg ggcgcgacau gguggccgag      600

gaagcaucgc uccuguuuca gagacagagg gagcuuggau gcgcuugggc cacacccgaa      660

cugcaagcug aguauuuguc aauccuccug cgucagcgga gcuucgauga gggcccuggc      720

gggaacaguc cauacggagg uaaucaggua gagaaaaugg uuggccgaug uaccuuugaa      780

cccgacgaac cuagagccgc aaaggccgcu uauuccuucg aguacuuuuc uuuacugcag      840

aaaauuaacc auaucaggcu cguggaaaau ggggagacuc gcccacuuac acaacagcag      900

cggcagcaac uguugagccu agcgcacaag acccccgaug ucucccuggc aagaauaagg      960

aaagagcucg cccugccuga aacggugcag uucaacggag ugcgcugccg agcuaaugag     1020

acucuugaag agucagaaaa gaaggagaaa uuugccuguc ugccagcaua ccacaagaug     1080

cggaaagccu uggacggcgu cguuaagggu cguaucagua gccucucuau uucccagaga     1140

gaugcugccg caaccgcgcu gucauuauau aaaaacgagg acacacugag ggcuaagcua     1200

acugaagccg gguuccaagc accggagauc gacgcccucg cuggccugac cggauuuagc     1260

aaauucggcc aucuuaguuu gaaggccugc cgcaagcuga uuccccaccu cgaacagggg     1320

cugacauacg aucaggcaug uucugcugcc ggauaugacu ucaaagguca uggcgcgggg     1380

gagcgggccu uuacccuucc ugcugcagcc ccagagaugg aacagaucac gucccccgug     1440

guaagaaggg cuguggccca aacuauaaag gucgugaaug gaauuauccg agagauggau     1500

gcaagcccug cguggguucg cauugaacug gcccgggagu ugucgaaaac auucggcgaa     1560

agacaggaga uggacagguc uaugcgugag aacgcugccc agaaugaacg ccucaugcag     1620

gagcugcggg auaccuuuca cuuacugucc ccaacugguc aagaccuagu gaaguacaga     1680

cucuggaaag aacaggaugg ggucugcgca uauucacuga ggcgacuuga cguggagcgg     1740

uuguucgaac ccggcuacgu agauguugac cacaucgugc cguauagucu gagcuuugau     1800

gaccgcagau ccaacaaggu ccucgugcug ucuagcgaga acaggcagaa gggaaaucgg     1860

uuaccucugc aguaccuuca aggcaaacga agagaggacu ucaucgucug gacaaacuca     1920

agugugcgcg auuacaggaa gcgucagaau uugcuccggg aaaaacuguc cggggacgag     1980

gcugaaggau uuagacagag gaaccuacag gauacccaac auauggcccg cuuccuguau     2040

aauuacauua gcgaccaccu cgcauuugcc cagucugagg cucuggguaa gaaacgaguu     2100

uucgcggugu caggcgccgu aacgagucau cuuagaaagc gguggggguu gagcaagguc     2160

agggcagaug gagaccugca ccaugcuuua gaugccgccg ugauagcuug uaccacagac     2220

ggcaugaucc gccguauuuc cgguuauuac gggcacaucg aaggcgagua ucuccaggau     2280

gcagacggag ccggcucuca acacgcgaga acuaaagagc gguuucccgc uccauggccu     2340

agguuccgcg augaacugau aguucgacuu ucggagcagc ccggggaaca ucuguuggac     2400

auuaacccag aguuuuacug cgaguaugga accgaacaca ucagacccgu guucgucagc     2460

cggaugccua ggcgcaaggu gacaggccca ggucauaaag agacuaucaa gggggccgca     2520

gccgcugaug aaggacuccu gaccguaaga aaagcacuaa cagagcugaa gcucgacaag     2580

gacggcgaaa uuaaagauua cuacaugccg uccucagaca cccugcuuua ugaggccuug     2640

aaggcgcagc ugaggcgguu cgguggcgau aguaaaaagg cuuuugccga gcccuucuac     2700

aaaccuaagg cagacgggac uccaggaccc uuagugcgua aaguuaagac gacagaaaag     2760

gccacccuca cugucccugu gcacggcggg gcugccucua augauaccau ggugcgcguc     2820

gacguuuuuc uggugccagg agauggcuau uacugggugc ccguauauac agcagacacc     2880

cuuaaaccgg agcugccuaa ccgagcuguc gugcagggua agccauacag cgaauggaaa     2940

gagaugagag aagaggauuu caucuuuucc cuauauccca augaccucgu uuacguggag     3000

cacaagucag ggcugaaauu cacuuugcaa aacgccgaua gcacacugga aaagacgagg     3060

acccagacuu cugcguuugu cuacuucgug ggaggcucca uuaguacagc cagcauaacc     3120

auccggacac augacaaugc auaugguauu ccuucuuuag gcguuaagac ucucaaaacc     3180

uuucacaagu accaggugga uguacugggg aacgucuucc ccgugcauaa agagacgcgc     3240

cagaaguuua gauga                                                      3255


<210>  2721
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2721
augaagaaca cccuguacgg caucgggcuc gacauuggag uggccagcgu cgguugggcu       60

guugugggcc uuaaugggac aggagagccc guaggccugc acagauuggg ugugaggaua      120

uucgauaaag cagaacagcc uaagacuggg gaguccuuag ccgcgccacg gcgcauggcu      180

cgaggaaugc guagaaggcu gcggcgcaaa gcccuaagaa gggcagacgu cuaugcccuc      240

cuggaacggu cuggccuuuc aacgcgcgag gcucuggcac aaauguuuga agccggcggg      300

uuggaggaua ucuacgcucu ccgaaccaga gcccuggacg agccgguggg aaaggcggag      360

uucaguagga uucugcucca uuuagcacag cggcgugguu uuaaaagcaa ccgcagaaca      420

gccuccgaug gcgaagacgg gaggcugcuu gcugccguua augaaaaccg gcgacgcaug      480

gcacagggag gcuggagaac ugugggugag auguuguaua ggcacgaggc uuucgcccug      540

cauaagcgga auaaggcgga ugacuaccua ucuaccgucg ggcgcgauau gguggccgaa      600

gaggcaucgc uccuguuuca gagacaaagg gaacuuggau gcgcuugggc cacacccgag      660

cugcaggcug aauauuuguc aauccuccug cgucagcgga gcuucgacga gggcccuggc      720

ggggauaguc cauacggagg uaaccaggua gagaaaaugg uuggccgaug uaccuuugaa      780

cccgacgagc cuagagccgc aaaggccgcu uauuccuucg aauacuuuuc uuuacugcag      840

aaaauuaauc acaucaggcu cgcggagaac ggggaaacuc gcccacuuac acaaccccag      900

cggcagcaac uguugagccu agcacauaag accccugaua ugucccuggc cagaauaagg      960

aaagagcucg cucugccaga gacggugcag uucaauggag uccgcugccg agccaacgaa     1020

acucuugagg aaucagagaa gaaggaaaaa uuugcauguc ugccggccua ccacaagaug     1080

cggaaggcuu ugggcggugu ggugaaaggg cguaucagua gccucucuau uucccagaga     1140

gacgccgcag cgaccgcucu gucauuauau aaaaaugagg acacacugag ggccaagcua     1200

acugaggcag gcuuccaagc ccccgaaauc gaugcucucg ccggacugac cggcuuuagc     1260

aaauucgggc accuuaguuu gaaggcaugc cgcaagcuga uuccucaucu cgagcaggga     1320

cugacauacg accaggcuug uucugccgcg gguuaugauu ucaaaggcca cggggccgga     1380

gaacgggcuu uuacccuucc agcagccgcu cccgagaugg agcagaucac guccccuguc     1440

guuagaaggg ccguggcaca aacuauaaag guagugaacg gcauuauccg agaaauggac     1500

gcgagcccag ccuggguccg cauugagcug gcucgggaau ugucgaaaac auucggugag     1560

agacaggaaa uggauagguc uaugcgugag aaugccgcaa agaacgagcg ccucaugcag     1620

gaacugcggg acaccuuuca uuuacugucc cccacugggc aggaucuagu gaaauacaga     1680

cucuggaagg agcaagacgg cguuugcgcu uauucacugc agaggcuuga uguggaacag     1740

uuguucgagc cgggauacgu cgacguggau cacaucguac cuuauagucu gagcuuugac     1800

gaccgacggu ccaauaaggu ucucgugcug ucuagcgaaa accgccagaa aggcaacaga     1860

uuaccccugc aauaccuuca ggggaagagg cgggaggauu ucaucgucug gacaaauuca     1920

agugugcacg acuaccgaaa aagacagaac uugcuccgcg agaagcuguc cgaugacgaa     1980

gccgagggau uuaggcagcg uaaucuacaa gauacccagc auauggcacg guuccuguau     2040

aacuacauua gcgaccaccu cgccuuugcu cagucugaag cgcuggguaa aaagagaguc     2100

uucgccgugu caggcgcagu uacgagucau cuuaggaagc gcuggggguu gagcaaagug     2160

cgagcugaug gagaccugca ccaugccuua gaugccgcug uagucgcaug uaccacagac     2220

ggcaugauaa gacggaucuc cgguuauuac gggcacauug agggcgaaua uuuucaagau     2280

gccgacggag cgggcucuca gcacgcuagg acuaaggagc gcuucccagc cccuuggccc     2340

cguuuuagag augagcucau cgugcggcug ucggaacagc caggggagcc ccuucuggac     2400

auaaauccug cauucuacug cgaauaugga accgagcaua uuaggccagu uuucgugagc     2460

cgcaugccgc gaagaaaagu cacaggcccc ucccacaagg agacugugaa aggugccgcu     2520

gcagccgacg aaggguugcu caccguacgg aaggcgcuga cagagcuaaa gcuggauaaa     2580

gacggagaaa ucaaggauua cuacaugccu ucaagugaca cccuccugua ugaggcucuu     2640

aaagcccagu ugaggcgcuu uggcggugau ggcaagaaag cauucgccga accauuuuac     2700

aagcccaaag cugacgggac uccuggacca cuggugagaa agguuaagac gacagagaaa     2760

gccaccuuaa cuguccccgu gcauggcggg gcagcuucua acgauaccau ggugaggauc     2820

gacgucuucu uuaugccggg agauggcuau uacuggguuc cucucuaugu ggccgacaca     2880

cugaagccag agcuucccaa ucgggcggug guagcccaca aaccuuacag cgaauggcaa     2940

gagaugcgug aagaggauuu cgucuuuucc cuguauccca acgaccuagu guacguugaa     3000

cacaagucag agcucaaauu caccgugcag aaugcagaua gcacucugga gaagacacgc     3060

acgcaggcuu cugccuuugu cuacuucgug uccgguggga uuaguggagg caccaucacu     3120

guucgaacac augacaacag cuaugguauu ccaucuuugg gccugaagac cuuaaaaucc     3180

cucuguaagu accaggugga cguacugggg aaugucuuuc cugugcacaa agaaacaaga     3240

caaaaguuca gguga                                                      3255


<210>  2722
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2722
augaagaaca cccuguacgg caucgggcuc gacauuggag uggccagcgu cgguugggcu       60

guugugggcc uuaaugggac aggagagccc guaggccugc acagauuggg ugugaggaua      120

uucgauaaag cagaacagcc uaagacuggg gaguccuuag ccgcgccacg gcgcauggcu      180

cgaggaaugc guagaaggcu gcggcgcaaa gcccuaagaa gggcagacgu cuaugcccuc      240

cuggaacggu cuggccuuuc aacgcgcgag gaacuggcuc aaauguuuga ggcaggcggg      300

uuggaggaua ucuacgcccu ccgaaccaga gcucuggacg aaccgguggg aaaggccgag      360

uucaguagga uucugcucca uuuagcgcag cggcgugguu uuaaaagcaa ccgcagaaca      420

gcauccgaug gcgaagacgg gaggcugcuu gccgcuguua augagaaccg gcgacgcaug      480

gcccagggag gcuggagaac ugugggugag auguuguaua ggcacgaagc auucgcucug      540

cauaagcgga auaaggccga ugacuaccua ucuaccgucg ggcgcgauau gguggcggag      600

gaagccucga uccucuuuga gagacagagg gcaaugggau gcucaugggc ugcccccgaa      660

cugcaagcug aguaucuuag cauucuguug cgucagcgga guuucgacga gggcccuggc      720

gggaacuccc cauacggagg uaaucaggua gagaaaaugg uuggccgaug uacauuugaa      780

cccgaugaac cuagagccgc aaaggccgcu uauucuuucg aguacuuuag ccuccugcag      840

aaauuaaacc acaucaggcu ggcggaaaau ggggagaccc gcccacucac ucaaccccag      900

cggcagcagc uucuguccuu ggcacauaag acaccugaca uaucacuagc cagaaucagg      960

aaagagcugg cucucccaga aaccgugcaa uucaacggag uccgcugccg agccaaugag     1020

acgcuggaag agagugaaaa gaaggagaaa uuugcauguc uuccggccua ccacaagaug     1080

cggaaagcuc uggauggcgu ggugaagggu cguauuagcu cuuuguccau cucacagaga     1140

gacgccgcag cgacugcucu cagccuguau aaaaacgagg acaccuuaag ggccaagcug     1200

acagaagcag gguuccaggc ccccgagauu gaugcucuag ccggccucac uggauuuagu     1260

aaauucggcc accugucucu uaaggcaugc cgcaaguuga ucccucaucu ggaacaaggg     1320

cucaccuacg accaggcuug uacagccgcg ggauaugauu ucaaagguca cggcgccggg     1380

gagcgggcuu uuacccugcc agcagccgcu cccgagaugg aacagauaac guccccuguc     1440

guuagaaggg ccguggcaca gacuauuaag guagugaaug gaaucauucg agagauggac     1500

gcgagcccag ccuggguccg caucgaacuu gcucgggagc ugucgaaaac auucggcgaa     1560

agacaagaga uggauagguc uaugcgugag aacgccgcac agaaugaacg cuugaugcag     1620

gagcuccggg acaccuuuca ucuguuaucc cccacugguc aggaucuggu gaaguacaga     1680

cuauggaaag aacaagacgg gguuugcgcu uauucacuca ggcgacugga uguggagcgg     1740

cuuuucgaac cgggcuacgu cgacguggau cacaucguac cuuauaguuu gagcuuugac     1800

gaccgcagau ccaacaaggu ucuggugcuc ucuagcgaga acaggcagaa gggaaaucgg     1860

cugcccuuac aguaccugca gggcaaacga agagaggauu ucauugucug gacaaacuca     1920

agugugcacg acuaccgcaa gaggcaaaau cuuuugcgug aaaaacucuc cggggaugag     1980

gccgaaggau uucggcagag aaaccugcag gacacccagc auauggcaag guuccuauau     2040

aauuacauaa gcgaucaccu ggccuuugcu caaucugagg cgcucgguaa gaaacgcguc     2100

uucgccgugu caggcgcagu uacgagucau cugcgaaaga gaugggggcu uagcaaggug     2160

cgggcugacg gagauuugca ccaugcccug gacgccgcug uagucgcaug uaccacagau     2220

ggcaugauca ggcgcauuuc cgguuauuac gggcacaucg aaggcgagua uuuacaggac     2280

gccgauggag cgggcucuca gcacgcucgu acuaaagaga gauuuccagc cccuuggccc     2340

cgguucaggg acgaacucau agugcgccug ucggagcaac cagcagaacc ccuucuggau     2400

auuaacccug aguuuuacug cgaguauggg accgaacaua uccgaccagu uuucgugagc     2460

agaaugccgc ggaggaaggu cacaggaccc ggccacaaag agacuaucaa gggugccgcu     2520

gcagccgacg aaggguugcu caccgugcgc aaagcgcuga cagagcuaaa gcuggacaag     2580

gauggagaaa uuaaagacua cuacaugccu uccucagaua cccuccugua ugaggcucuu     2640

aaggcccagu ugagaagguu cggcggugac ggcaaaaagg cauuugccga gccauucuac     2700

aaacccaagg cugaugggac uccuggacca cugguacgga aagugaagac gacagaaaag     2760

gccaccuuaa cuguucccgu ccauggcggg gcagcuagua augacaccau ggugcgugug     2820

gaugucuuuc ucguuccggg agacggcuau uacugggugc cuguguauac agccgauacc     2880

cugaaaccag agcuucccaa ccgcgcggua guccagggua agccuuacuc ugaauggaaa     2940

gagaugcgag aagaggacuu caucuuuagc cuguauccca augaucuagu guacguugag     3000

cacaaguccg ggcucaaauu cacucugcag aacgccgacu caacauugga aaagacgaga     3060

acccaaacua gcgcauuugc uuacuucgug ucuggaucca uuaguacagc cagcauaacc     3120

aucaggacac acgauaaugc uuauggcauu ccaucucugg gugucaagac uuuaaaaacc     3180

uuucauaagu accaggugga cguucucggc aacguguucc cuguacacaa agagacgcgg     3240

cagaaguuuc gcuga                                                      3255


<210>  2723
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2723
augagacugg gccucgacau cgggaccaac agcauuggau gguggcuuua cgguacagag       60

ggcgggagga uaacugaagu ggucgaugga ggcguucgga ucuucuccga cggucgcgau      120

ccccagucua aggccucacu ggcuguggac cgacgugcag ccagaaguca aaggcggcgc      180

agagauaggu auuugcggcg caaagcggcu uuaaugaagc gaauggccca gguagggcug      240

augccugcag acccagccga ggcuaaagug cuagaagucc ucaauccgua cgagcugaga      300

gcaagcggac uugauggccc ccugccuuug acgcacuuug gcagggcccu cuuccaucug      360

aaccagcggc guggguuuaa guccaaucgc aaaaccgaca gaggagauaa cgaaucuggu      420

aagauuaagg acgcuacagc caggcuggau caggcgauga uggcaaaagg cgcccggacu      480

uauggggagu uccuccacau gcgacgcgcu gccgcauuag acccaaaguc ggugcccuca      540

guuagaacca ggcugacagu ggcucggcgc gaugacgccg agaaagaaga ggcgggauac      600

gacuuuuauc cugauagaaa gcaucuuagc gaagaguucg acaaauugug ggccgcacaa      660

gcuaggcacg ccccagaugu ccugaccgag gaccuacgug augaaaucgc uacuauuauc      720

uuucaucagc ggccccucaa gacaccugag gugggccugu gccuuuucac cggucagcac      780

gggguaccag aacgagacaa gagaauaccg agugcccacc cccugaauca gaggcgcauc      840

uugauggaga cgguuaacaa ucucaaagug gcagcccaag gagaaccuuc ccggccacug      900

acuagagagg agagggauca gauuguccau gcuuuagaca acaagaaaca caccaagucu      960

cugagcggca ugacaaugaa acucaaggcg cuuggcaaag ugaucaagcu gcgccccgaa     1020

cagggguuua cuuuggagac cgcaaaucga gaugccauug cuugugaccc ugugcgggcc     1080

ucccuaucac auccagaucg uuucggagca agauggagua cacuggaaag cgccggucag     1140

ugggacgucg uucaaaggau ccgcgcugug cagucugacg ccgagcacgc agaucucgua     1200

ggcuggcuga uggacgugca cgggcuugau cgggaccaug cggaagagac cgcuaacgca     1260

ccccugccgg agggauacgg cagauugggu cucacggcca cuacaaggau acuggccgcu     1320

uuagaagccg auguccugaa uuauuccgca gcuguggccg cgugcgggug gcaccauuca     1380

gacggccgaa ccggagaggu ucuagaaagc cucccuuacu acggcgagau ucuggaacgc     1440

cacgugaucc ccgggacugg agauccaaac gacgaugacg ucaagcggua ugguagaauu     1500

acaaauccua ccgugcauau cggccuuaac caguugaggc gucugguaaa ucgcaucauu     1560

acgguuuacg ggcggcccga ugagauagug gucgagcucg ccagagaccu gaaacuuagu     1620

gaagaucaaa agaaagacgu gcagagggac aucaagaaaa acaccgaggc ugcaauucga     1680

cggggaaaga agcuggaaga gaugggcguc ccaaacacag gugccaaucg caugguguug     1740

agacucuggg aggaucuggg ggacgauguu augacuagga acugucccua uaccggcaaa     1800

cggaucucug cuggaauguu auuugacggc uccugcgaug uggaccacau acugccuuac     1860

agccgaacac uagaugacuc guucgccaau agaacucucu gucugaagga agcaaaccgc     1920

cagaaaaccg auaagacacc augggaggcg uggggcgaaa ccgagcagug ggaagccauu     1980

gcugccaauc uuaaaaacuu gccggcaaau aaggcuugga gguuugcccc cgacgcaaug     2040

gagcguuucg agggagaaaa ggauuucucu gcccgggcuc uggcggacac ucaauaucuc     2100

uccagaaucg ccagggcaua ccuggaugcu uuauaugacg gugacgaugg caaaucacac     2160

guaugggucg ugccugggcg ccugacggag augcuucgaa gacauugggg auugaacggc     2220

guugccgccc ucacagacag ugauaagcag accgugaaag cuaagaaucg gacugaccac     2280

aggcaucacg caaucgaugc cgcggucgug gcugccaccg accgcggucu gauuaaacgu     2340

aucgcagaua uggccaagag agacgaauuu aacggggcug aggaaguagc acggagcauu     2400

ccacccccuu gggagggcuu cagggaugac guguccgccc agguuacacg cauaaucguc     2460

ucucaccgag cggaucaugg aagaauugac ccagcugccc ggaaaagcgg cagggacuca     2520

accagugggc agcuacacaa ugauacugca uacggaaucg gccgcgacgg uacaguggug     2580

uccagaaaac cccuggccag ccucucuccg aacgauaucg ucguuacgaa gaggggagcu     2640

aauauucggg acguggaucu gcaaaaacau cuugcccgug ugacccacgg guuggacggc     2700

aaggcauuug aggcugcccu guuagcguuc gccgcaucac cuauacuccc agauaacacu     2760

gacaaucccu acuuuggucu gcgccgagua agacuugaag agacacugca ggaaagugcu     2820

agggucgagg uggccgauca ggaaggccgg ccuuauaagg cuuacaaggg ggacagcaac     2880

cauugcuaug agaucuggug uuugcccgau ggaaaaauua agccacaggu ugugaccaca     2940

uacgaggcac accaauccgg cgccgaaaag aaaccucacc ccgccgcuaa gcgccucaug     3000

agaaucuuca aaagggacau ggucaugcug gagcgggaug gggaaacuau uaucggauau     3060

gugcagaaga ugaaacagaa cggcucuaua uuuguugcgc cacauaccga ggcaaaugcc     3120

gacgcucgag acagagaucc gaaggacgau uucaagcuaa uccagcuggg ugccgggucg     3180

cucgugaaag caaaggcccg caggguaauu gucgacgaga ugggacggcu gcgugauccu     3240

gggcccccac uuuga                                                      3255


<210>  2724
<211>  3258
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2724
auggacaagg gcaccaacag caucgggugg gccgcucuga gacucgauga gcagggagaa       60

cccgugggua uuauagaggc aggcgucagg aucuacgccg acgggcggga accuaaaucc      120

ggaacaucuc uugcggaggg ccgccgaauu gcucguggua ugucaagaag gcgggaucgc      180

uauaagagaa ggcggcgcac uguucugaau acguugaccg aguucggguu aaugccagcc      240

acaaaagcag cccgaaaggc ucugguggca gaaacuaacg acagaagguu uggagaggua      300

gccaguaaug uguacgcucu acgggcccgu gcgcucgaug aaaaacugcc gcuuuaucaa      360

cugggccgcg cauuguucca ccucaaccag agaaggggcu uuaagagcaa ucggaagacc      420

gacaaacgag auaacgacca ggggaagauc gccuccgcua uuucucgccu gggaagaaaa      480

cuggaugagg ccggugcaag gacacucggc gaguucuuac auaagcagca cgcugccggg      540

aaaugggucc gggugcgccc cucgagaguu ccuggagaag acgauaagga ggaagacggc      600

uacgguuuuu auccagagag gucauuccug gaacgugagu uugcggccau cugggaagca      660

caagcugccu ucuaccccga gcuuuugacc gaggaacggc gagaucaucu gcacugggug      720

auguuuuucc agagaccucu aaagaggcca acugucggga aaugcagcuu ucaucccgac      780

gaggaccgcc ucccuaaggc ucacccacug uuccaggaau uucggcuuau aaaagagcug      840

aaugaauugg aggugguaca cagaggagau aggcgcgaga agcucacacc ggaccagcga      900

gaucggcugu ucaaccaauu acguacccag agaaguuccg ccuucacggc acugaggaaa      960

acuaucaagg cccccaccgg cacacgcuuu aauaaagaau cugacgcucg ggauaagcuc     1020

aagggcgacg agguugcggc agaaaugagc cauaaagaua gauucgggca ggccuggauu     1080

ggaaugccuc uugcugagag gugggccgug gucgcaaugc uguuggacac ugaagaucca     1140

cuagagguga aggccuggcu ggaggaacga uaugguuuug cugccgaccg caucgaugca     1200

auugcggcug ccaaacuccc caccggcuac gggcggcugg gagagacagc acuuagagac     1260

augcuggacg aauugaggaa cggcaccgug aaggaugacg ccggucguga ugucguucuc     1320

acggagcgcc aggcuaugga caauaucugg ggcgauaacc cugcccugca acgggcagug     1380

acugacgcua gagaguuacc acccuaccag gaaguacugg ggaggcgaau accgccugga     1440

acaggcaaac ccgccgaucc auaugacgug cagaaggggc ggcuaaccaa uccuacuguc     1500

cacauuggac ucaaccaacu gaagcgcauc gugaauguuc uuaugagaag gcauggucgg     1560

cccgagcaga uugcguugga acuggcccga gaucucaaac ugaacgagga acagaaggcu     1620

gagaaaaaca gagagaucgc acgcaauaca aaggccgcug aagccagggg caaaaagcug     1680

gaggaacuug ggcacgcaaa caauggauac aaccgugugu ugcucaagcu gugggaggac     1740

uuaaaucuga aacagccaga aaaccgggag uguaucuauu gcggcaaggc gauugguaua     1800

gggacccuac ucuccaauca agucgauauc gaccacauuc ugcccugguc aaaaacgcuu     1860

gaugacaguc aggccaacag aaucgugugu cauguagagu gcaauaggca gaagggcaac     1920

cacgcuccug ccgaaguucc acaguggcau gaccgcuacg augacauauu ggcacgagcu     1980

gagagacugc cgcggaauaa agccuggagg uucggacgcg aagcaaugaa gaaguuuaaa     2040

gaugacgaga gcuuccucgc ccgucaacug accgauacac aguauuuauc uagacuggcu     2100

cacgaauacc uuggcgcguu guaucccgac gaggagccug augaaugggg agggccagcc     2160

cggaggaacc augugcgcgu cauucccggu cgaaugacug agaugcucag acggcagugg     2220

ggccugaaug caaucaucuc cgaccacaac cagaagaaua ggaacgauca ccgccaucac     2280

gcuauugacg ccaucguggu cggggugacc acaagaaggc uacugcaacg gauugccacu     2340

guugcuggag gccgugaagc acucgcccug gaggagacca uagguaaaau cgaaccucca     2400

uggcccgggu uuuacgauga cgugaaggcg gcuauugauc gcaucguagu cucacauaaa     2460

ccggaccacg gcagccuucc uaagccagga aaacccggcc gaacagccgg gcaguugcac     2520

aacgacaccg cauacggauu cacuggcgag gccagaaaug gugugccuau caugguucau     2580

agggugcccu uugcuagucu gacggaaaag gauauuccau cuauacggga ccgccaguua     2640

gcagaugccc ucuaugcggc uaucuccggg auuagcggaa aggccgagcu ggcagccgcu     2700

cuugccagau ucucggaauc uggcccuuac aaagguauca ggcacguccg ggugguagag     2760

cccguguccg uuauugcaau caaggaccag gcuggcaaag ccuauaaggg guacaaagga     2820

gaugcgaacu aucgauacga cgucugggag cugccaaaug gcaaguggac acaugaagug     2880

gugucaaugu uuaacgccca cagaccggau uggcaaagug agauacgcag caagaauccu     2940

gcagcuagga aagucuuguc ccuccaucag aacgacaugg uugccuauga acacccccag     3000

gauggguaca ccaucggacg gguggugaag uucucugcug gcggucaaau ggcacuggcc     3060

gggaaucgug agggaggcgc ccuaaaaagc agagacgcug auccagaaga cagguuucgc     3120

uacuucuaua agucaggugg cgcgaugcgg gacguacagc ugcgacaggu cagaguggau     3180

gagacuggga ggguuuucga ccccggaccu caggaucgcg agagucggaa cgcaagaaaa     3240

caaaaugacg ccggcuga                                                   3258


<210>  2725
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2725
augagauaca agaucggccu ggacauuggg auaaccagcg ugggaugggc cgucaugaac       60

cucgauaucc ccaggauuga ggaccuuggu guucggaucu ucgaucgcgc ugaaaauccu      120

cagacaggcg agucccuggc auugccacga cguuuagcca gaucugcgag gcggcgccug      180

agaaggcgga aacaccgccu agaacgaauu agaaggcucg ugauccggga ggggauacug      240

acuaaggaag aacuugacaa acuguuugag gaaaagcaug agaucgaugu auggcaauug      300

cguguggaag cucucgaccg caaacugaac aaugaugagc uggccagagu ccucuuacac      360

cuggcaaaga ggcggggauu caagucaaac cgaaaaagug agcgcagcaa uaaggaaaac      420

uccacgaugc uuaaacauau ugaggaaaau agagccaucu ugucuucgua uaggaccgug      480

ggcgagauga uuguuaagga cccgaaauuu gcucugcaca agcggaacaa gggugaaaau      540

uacacaaaca cuaucgcacg cgaugaccua gagagagaga uaaggcucau uuucucaaaa      600

cagcgugaau uugggaauau gagcugcacc gaggaguucg agaacgaaua uaucacaauu      660

ugggccaguc agcggcccgu ggcuuccaag gaugacaucg agaaaaaggu cggauuuugu      720

accuucgagc cuaaagaaaa gcgagcccca aaagcgacuu acacauuuca gucuuucauc      780

gcaugggagc auauuaauaa gcugagacuu auaagccccu ccggcgccag gggccugacc      840

gaugaggaac gccgguugcu cuaugaacag gcuuuucaaa agaacaaaau cacguaccac      900

gacauuagaa cucuguuaca ucugccugac gauaccuauu ucaaggggau cguguacgac      960

aggggagagu cacgcaaaca gaaugagaac auacgauuuc ucgaacuuga ugccuaccac     1020

cagauucgga aggcaguaga caaaguuuau gguaagggca agaguagcuc uuuccugcca     1080

aucgauuucg acacauuugg guacgcuuug acucuauuca aagaugacgc cgauauccac     1140

uccuaucugc guaacgagua cgaacaaaau ggaaagagaa ugcccaaccu cgcgaauaaa     1200

guguaugaca acgagcugau ugaggaacuu cugaauuugu cauuuaccaa guucggccau     1260

cucagccuga aagccuuaag gaguauccug ccuuacaugg agcaggguga agucuauucu     1320

uccgcaugcg agcgcgcugg guacacauuu accggaccaa agaagaaaca gaagacgaug     1380

cuacucccga acauuccccc uauagccaau ccagugguga ugcgggcucu gacucaggcc     1440

agaaaagucg uuaacgcaau cauuaagaaa uacggcagcc ccgugucgau ccacaucgaa     1500

cuugccaggg auuugucuca aacauucgac gagcgacgca agaccaagaa agagcaggac     1560

gaaaaucgga agaaaaacga gacugcuauu agacagcuga uggaauaugg ccucacacug     1620

aauccuaccg ggcacgauau aguaaaguuu aaacuuuggu ccgagcaaaa cggaaggugu     1680

gcguacucac ugcagccaau cgaaauugag cguuugcucg agcccgguua uguggaaguc     1740

gaccauguga ucccguacag ucgcagccug gaugacuccu auacgaauaa gguuuuagug     1800

cugacccggg agaacagaga aaaaggcaau aggauuccug cagaguaccu aggggucgga     1860

acagaacgau ggcagcaguu cgagacuuuu gugcucacca acaagcaauu cucuaagaaa     1920

aagcgggauc gccugcuuag auugcacuau gacgagaacg aagagacaga auuuaaaaau     1980

aggaaccuga augauacucg guacaucagc cgauucuuug ccaacuucau aagagagcau     2040

cucaaguucg cugagucaga cgauaaacag aagguauaca ccguuaaugg ccgcgugaca     2100

gcccaccuga ggagucguug ggaauuuaac aagaaucggg aggaauccga cuuacaucac     2160

gcagucgaug ccgugaucgu cgcuugcacc acucccagcg acauugccaa agugacggca     2220

uucuaucaga gaagggagca gaacaaggaa cuggcgaaaa agaaagagcc acacuuuccu     2280

caacccuggc cacauuucgc ugaugagcuu cgcgcccgau ugucuaagca ccccaaggaa     2340

ucaaucaaag cacucaaucu ggguaacuac gacgaccaga agcuagagag ucugcagccu     2400

guuuuuguga gcagaaugcc aaaacggucc guaacagggg ccgcucauca ggaaacccuc     2460

aggcgcuaug ucggcauuga ugagcguucu ggaaagaucc aaacuguggu uaaaaccaag     2520

cugucggaaa uaaaacuuga cgccagcggc cacuucccga uguacgggaa agaguccgau     2580

cccagaacau augaggcaau ucggcagagg uugcuggaac acaauaacga cccuaagaag     2640

gcuuuucagg agccauuaua caagcccaaa aagaauggag aaccuggucc agugauccgc     2700

accgucaaaa uuaucgauac uaagaaccag gugauacccc ucaaugacgg caagacagua     2760

gccuauaacu caaacaucgu gcgaguugau gucuucgaga aagacgggaa guacuacugu     2820

gugccggugu auacgaugga uauuaugaaa ggaauccugc cuaauaaggc gauugaacca     2880

aacaaacccu acagugagug gaaggagaug accgaagacu auacuuuuag auucucucuu     2940

uacccuaaug aucugauccg gauagaguug cccagggaaa agacagucaa aaccgccgcu     3000

ggcgaggaga uuaacguuaa ggacguguuu guguauuaca aaacaaucga uagcgcaaau     3060

ggugggcucg aacugaucuc ccaugaccac cgcuucucac uaagaggcgu aggaagcagg     3120

acucugaagc gguuugagaa auaucaaguc gacgugcucg gcaacauuua caagguucgu     3180

ggggaaaagc gcgugggacu ggccucuucc gcucauagua aaccagguaa gaccauccga     3240

ccucuucaga gcacgagaga uuga                                            3264


<210>  2726
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2726
augcugagaa ugaaguacgc caucggccuc gacauuggga uagcuagcgu gggauaugca       60

guccuugccc uggaucacga ggaaaacccc ugggguauca uuagguuggg cucccggauc      120

uucgacguug cggagaaucc uaaagauggg gcuucuuuag cccugccacg ccgagaagca      180

cguucaguga gaaggcggcu acgcagacau caccauaggc ucgagcggau uaagaaccug      240

cuuaucaaua ccgaauuuau aacaaaagac gagcuguugc accucuacga uggaaaccug      300

agugacguau augagcugcg cgugaaggcc cucgauuaca gcgucacuaa ugcugaauua      360

acgcgaaucc ugcuucauuu ggcacagaga aggggcuuca aauccaaccg gaagucugac      420

gccaaugaua aggaggcugg ucaacugcua gaagccgugu cggcgaacgc aaaacguaug      480

caggagaauc acuaucgcac cguuggggag auguuuuaca aggacgaucu cuucucaaaa      540

uauaagagaa acaaaggagg cacauaccug acuaccgugc acagggacau gauugccgcu      600

gaagcccgga caauccuuga gaagcaguuu gcacugggca auaacauuug cacccaggau      660

uucaucgaca aguacuugag cauacuccug agucaacgac aguuugacga agggccggga      720

gagcccuccc cuuaugcugg uaaucagauu gccaacauga ucggcaaaug uacuuucgaa      780

ccaaaugagu accgcgcggc caaggcaucu uauagcuuug agagauucaa cuuacugcag      840

aaagucaacc aucucaggcu ucuguuggaa gggaagucca uugcucuaga uaaugagcaa      900

cggaaaaaga ucaucgcccu ggcucacgac aaagccgauc uccgcuacuc acauauuaga      960

aaggcacugg gacuugacga aaaggugcug uuuaacacaa uaaccuauaa ugaugacgua     1020

aguguuaucg agaaaaagac gaaauucaac uuuuugcagg ccuaccacca gauuaagaaa     1080

gugcucgcug aagauaugca gaagcugacu accgagcaau uagacaauau cggccagaua     1140

cugagcacau auaagucuga uaacaaaagg acugaggaac uauccgcgcu cggucuggag     1200

aagaaaauua ucgacgcacu uuuggggauc aauggacugu caaaguucgc ccaccucagc     1260

cugaaagcuc ugcguaagau uaaccccuac cuugaagagg gcaagaucua caaugaagcc     1320

ugcaccgcag ccggcuauga uuucaaagcu caugccaaca cacagaagac cgaguugcuc     1380

ccugcauaca aagaggaaau ggacgacauu acgaguccag ucgcgcggcg agcuauagcc     1440

caaucuauca aggugauuaa ugcaaucauc agagagcaga aagggagccc ccuguauauu     1500

aacauagaau uagccaggga gauggcuaag ggauuugaug agcgcacuca gaucgacaag     1560

gccaauaaag aaaaccaggc aaagaaugag cggauuaugg aaagaaucag gacagaguuc     1620

cacaaagcua acccuaccgg uauggaucug auuaagcuaa aacucuggga ggaacaagac     1680

ggccgcuccc cauacucgca gaaggccauc ucuauaaacc gacuguuuga gccgggguau     1740

guggauaucg accauauugu ccccuacucc aucucauucg augacaguuu uaaaaauaag     1800

guucuugugu ucagcgauga aaaccgggac aagggaaauc guuugccuau ugcguaucug     1860

caguccaaau uuggcgccaa ggcugcagag aacuucauga ucuggguaca gucuaauaua     1920

aaagauuaca agaaaagaca aaagcuccug aagagggaaa uuacugagga agacaucaac     1980

aaauuuaagg agcgcaauuu acaggauaca aaaaccauua gccgguuccu guacaacuau     2040

aucaaugacu accuuuuguu ugccccauca gacacgggua agaaaaagag agugaccgcu     2100

gucaacggga caauaacugc cuaucucagg aagcgauggg gcaucaauaa aauucgcgca     2160

aacggagaua agcaccaugc gguggacgcc guugugaucg cuuguaccac agauaaaaug     2220

auuaaggacc ugaguuccuu cagccgguac cacgagcuag aauauacuca caccgauaca     2280

gagucuuuuc uggucaauuc acucaccggc gaaauccuga aaagauuccc cuacccuugg     2340

gaggacuuca ggccagaacu uauggcccgu uugagugaua accccgcaga cgcucugcgc     2400

aaguuaaauc ucauauuuua ucaugggauu gaucugagca cuaucaagcc gaucuucgug     2460

ucccggaugc cuagacacaa aguaacggga gccgcacaua aggccacaau uaaaucugcu     2520

aggugccuug acaacgguau cguuauuugu aagaccucgc ugcagaacuu gaaacucgau     2580

aaggagggcg agauagcgaa uuacuacgcc cccgaaagcg acacuauccu guauaacgca     2640

cuaaaagagc gacugcgguc cuacgauaau aagccagcua aggccuuugc cgaaccuuuc     2700

uauaaaccca aggcugacgg gaccccagga ccccucguga aaaaggucaa aguguacgag     2760

aagucaacac ugaacgucgc agugcagcaa aauaccgccg uugcggacaa cgauaguaug     2820

gugcgcguag acgucuuuca cgugaagggc aaugguuauu accuuguucc uauuuauauc     2880

gcugauacuu ugaaaaagga gcugccaaac aaagccauug uggcauacaa gccguacgcc     2940

gacuggcccg ucauggauga cucuaauuuc aucuuuagcu uauauccuaa cgaucucaua     3000

aaauucgaac acaaaaauuc cgugaaguuu gcuaagguaa acaaggaguc aacacugaau     3060

gacuucuaca gcacgaaauc ugaaaucgug uauuuuaagg ggugcaacau uaauaccggc     3120

gcaaucacua uuaucaacca ugagaacaca uacguugucu ccagucuugg agugaaaacc     3180

cugccaagcc uagaaaagua ucaggccgau gugcucggca auuacacaaa ggucaaaaag     3240

gagguuagaa aacccuucag guga                                            3264


<210>  2727
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2727
augagauaca agaucggccu ggacauuggg auaaccagcg ugggaugggc cgucaucaac       60

cucgauauuc ccaggaucga ggaccuuggu guucggauuu ucgaucgcgc ugaaaauccu      120

cagacaggcg agucccuggc auugccacga cguuuagcca gaucugcgag gcggcgccug      180

agaaggcgga aacaccgccu agaacgaauc agaaggcuca uaauccggga ggggauucug      240

acuaaggaag aacuugacaa acuguuugag gaaaagcaug agaucgaugu guggcaauug      300

cguguagaag cucucgaccg caaacugaac aaugaugagc uggccagagu gcucuuacac      360

cuggcaaaga ggcggggauu caagucaaac cgaaaaagug agcgcagcaa uaaggaaaac      420

uccacgaugc uuaaacauau ugaggaaaau agagccaucu ugucuucgua uaggaccguc      480

ggcgagauga uagugaagga cccgaaauuu gcucugcaca agcggaacaa gggugaaaau      540

uacacaaaca cuauugcacg cgaugaccua gagagagaga ucaggcucau uuucucaaaa      600

cagcgugaau uugggaauau gagcugcacc gaggaguucg agaacgaaua uaucgccauc      660

ugggcuaguc agcggcccgu ugccuccaag gaugacauug agaaaaaggu gggauuuugu      720

acauucgagc cuaaagaaaa gcgagcgcca aaagcaaccu acacuuuuca gucuuucaua      780

gccugggagc auaucaauaa gcugagacuu auuagccccu ccggcacaag gggccugacc      840

gaugaagaac gccgguugcu cuaugaacag gcuuuucaaa agaacaaaau cacguaccac      900

gacauaagaa cucuguuaca ucugccugac gauaccuauu ucaaggggau ugucuacgac      960

aggggagagu cacgcaaaca gaaugagaac auccgauuuc ucgaacuuga ugccuaccac     1020

cagauccgga aggcagugga caaaguauau gguaagggca agaguagcuc uuuccugcca     1080

auugauuucg acacauuugg guacgcuuug acucuauuca aagaugacgc cgauauccgu     1140

uccuaucuga gaaacgagua cgaacaaaau ggaaagagga ugcccaaccu cgcgaauaaa     1200

guuuaugaca acgagcugau ugaggaacuu cugaauuugu cauuuaccaa guucggccac     1260

cucagccuga aagccuuacg caguauacug ccuuacaugg agcaggguga aguguauucu     1320

uccgcaugcg agcgggcugg guacacauuu accggaccaa agaagaaaca gaagacgaug     1380

cuacucccga acauuccccc uaucgccaau ccagucguga ugagagcucu gacucaggcc     1440

aggaaagugg ucaacgcaau caucaagaaa uacggcagcc ccguuucgau ucauauagaa     1500

cuugcccgag auuugucuca aacauucgac gagcgccgga agaccaagaa agagcaggac     1560

gaaaauagaa agaaaaacga gacugcuauc aggcagcuga uggaauaugg ccucacacug     1620

aauccuaccg ggcacgauau ugugaaguuu aaacuuuggu ccgagcaaaa cggacguugu     1680

gcguacucac ugcagccaau cgaaauugag cgcuugcucg agcccgguua uacggaagua     1740

gaccacguga ucccguacag ucggagccug gaugacuccu auaccaauaa ggucuuagug     1800

cugacaaaag agaacagaga aaagggcaau aggauaccug cagaguaccu agggguugga     1860

acugaacgau ggcagcaguu cgagaccuuu gugcucacaa acaagcaauu cucuaaaaag     1920

aaacgggauc gccugcuuag auugcauuau gacgagaacg aagagacuga auuuaagaau     1980

aggaaccuga augauacccg guacaucagc cgauucuuug ccaacuucau uagagagcac     2040

cucaaauucg cugagucaga cgauaagcag aaggucuaca cagugaaugg ccgcguaacc     2100

gcccaucuga ggagucguug ggaauuuaac aaaaaucggg aggaauccga cuuacaccau     2160

gcaguugaug ccgugaucgu cgcuugcacu acgcccagcg acauugccaa ggugacagca     2220

uucuaucaga gaagggagca gaacaaagaa cuggcgaaga aaaccgagcc acacuuuccu     2280

caacccuggc cacacuucgc ugaugagcuu cgcgcccgau ugucuaagca ucccaaggaa     2340

ucaaucaaag cacucaaucu ggguaacuac gacgaccaga agcuagagag ucugcagccu     2400

gucuuuguga gcagaaugcc aaaacggucc guuacugggg ccgcucacca ggaaacccuc     2460

aggcgcuaug ugggcauaga ugagcguucu ggaaagauuc aaacaguagu caaaaccaag     2520

cugucggaaa ucaaacuuga cgccagcggc cauuucccga uguacgggaa agaguccgau     2580

cccagaacuu augaggcaau ucggcagagg uugcuggaac acaauaacga cccuaagaag     2640

gcuuuucagg agccauuaua caagcccaaa aagaauggag aaccuggucc agugauccgc     2700

acaguuaaaa uaaucgauac gaagaaccag gugauucccc ucaaugacgg caagaccguc     2760

gccuauaacu caaacaucgu gcgaguagau guguucgaga aagacgggaa guacuacugu     2820

guuccggucu auacuaugga uauuaugaaa ggaauccugc cuaauaaggc gauagaacca     2880

aacaaacccu acagugagug gaaggagaug acagaagacu auaccuuuag auucucucuu     2940

uacccuaaug aucugauucg gaucgaguug cccagggaaa agaucauuaa aacagccggc     3000

ggugaggaga ucaagauuaa agaccucuuu gcuuauuaca agacuauaca cagcgggacc     3060

gcaggccugg agcuaguguc ccaugauugc ucauucagcc ugucuggagu gggcucccgc     3120

acgcucaaaa gauuugagaa guaucaaguc gacguucugg ggaacaucua caaggugagg     3180

ggagaaaaac gggugggucu ugccaguagc gcucacucua agaccggcga gacaauucgu     3240

ccauugcagu ccacucgcga cuga                                            3264


<210>  2728
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2728
augagauaca agaucggccu ggacauuggg auaaccagcg ugggaugggc cgucaucaac       60

cucgaucuuc ccaggauuga ggaccugggu guucggaucu ucgaucgcgc ugaaaauccu      120

cagacaggcg aguccuuggc auuaccacga cgucuggcca gaucugcgag gcggcgccua      180

agaaggcggc gccaccgacu cgaaagaauu aggcggcugc uuacucguga gaacgugcug      240

acgcaagaag aguuggacaa acucuuugag aagaaacaug aaaucgaugu auggcagcug      300

cgcguggagg cucuggacag aaagcucaau aacgaugaau uagccagggu ccugcuucac      360

uuggcaaaac ggcgaggguu caagucaaau cgcaagagug agagaagcaa caaagaaaau      420

uccaccaugc ugaagcauau agaggagaac aggucuaucc uaacaggaua ucggacugug      480

ggcgaaauga uuguucgcga cccgaaauuu gugcuccaca agagaaauaa aggugagaac      540

uacaccaaua caaucgccag ggaugaccug gaacgugaga uucagcuuau cuucgcucgg      600

cagcgagagu uugggaacau gauaugcacc gaagcauucg agaaugaaua uauugccauc      660

uggucgucac aaagacccgu cgcuagcaag gaugacauug agaagaaagu gggauuuugu      720

acuuucgaac cuaaggagaa aagggcccca aaggcgacau acaccuuuca gaguuucauc      780

gcaugggagc auaucaacaa acugcgcuug guaucccccu cuggcacgcg ggaacucacu      840

gaugaggaaa gaaggcuguu auaugagcag gccuuucaga agaauaaacu gaccuaccac      900

gacauucgca cacuccuuca ccuuccugac gauacuuauu ucaagggcau aguuuacgac      960

uggggagaac cauugaagca aaacgagaac auccgauuuc uagagcugga ugcuuaccau     1020

cagauucgga aaaccgugga caaggucuau gggcguggua aaagcuccuc auuccucccc     1080

aucgauuucg acacauuugg cuacgcccug acccuuuuca aggaugacgc acacauaaga     1140

aguuaucuga ggaaugaaua cgagcagaac gggaaacgca uugcuaauuu ggccaacaag     1200

guguaugaua augaacucau cgaggagcug uuaaaccuga gcuuuacgaa guucggacau     1260

cuaucucuca aagcgcugcg gaauauccuu ccuuacaugg aacagggcga gguguauucc     1320

ucagccugcg aaagagcagg uuacacuuuu acagggccaa ccaagaaaca aaagacuguc     1380

uugcugccga caauuccccc uaucgcuaac ccaguuguga ugagggcccu cacccaggcu     1440

cgaaaaguag ugaaugccau uauaaagaag uacggaagcc ccgucaguau ccacauugag     1500

cuggcacgcg accuuucuca gacguucgau gaacggaaaa agaccaaaaa ggagcaagac     1560

gagaacagaa aaaggaauga aacagccauc cgucagcuga ucgaguaugg cuugacucgc     1620

aacccuaccg gccacgacau ugugaaguuu aagcucuggu ccgaacagaa ugggcggugu     1680

gcuuacagcc ugcagccaau agagaucaaa agauuacugg aacccggaua uguugaggug     1740

gaucauguca uuccguacuc gaggucucua gacgauuccu auacaaacaa ggugcucgua     1800

cugacuaaag agaaucgaga aaaggguaac cggaucccug cggaguaccu uggcguuggg     1860

accgaacgcu ggcaacaguu cgagacauuu guguugacca acaaacaguu cucaaagaaa     1920

aagagagaca ggcugcuccg gcugcacuau gaugaaaaug aggaggcaga auggaagaac     1980

cgaaguuuaa augacacuag auacauuagc cgcuuuuucg ccaacuuuau cagggagcau     2040

cugaaauucg cugauuccga cgauaagcag aaagucuaca cggugaaugg aggcgucaca     2100

gcccaccuuc guucucggug ggacuucaac aagaauagag aagagagcga uuugcaucac     2160

gcaguggacg ccgcuauagu ugccugcacc acucucucag auaucgcaag ggugaccgcg     2220

uuuuaucaac gccgagagca ggcuaaagaa cuggccaaga agacagagcc ccacuuccca     2280

cagccuuggc cccauuuugc agacgaacua agagcccggc ugaguaaaaa cccaaaggag     2340

uccauuaaag cucucaaucu ggguaacuac gacaaugaaa agcuggagag cuugcagccc     2400

guauucgucu cuaggaugcc uaaacgcuca gugaccgggg ccgcacacca agagacucug     2460

cguagauaua ucggcauuga ugaacggagu ggaaagaucc agacaguugu gaagacgaaa     2520

uuaagcgaga uaaagcucga cgcuuccggc cauuuuccaa uguacgggaa agaaucugau     2580

ccgaggaccu augaggccau ucgccugcga cuucuggaac acaacaauga ccccagaaag     2640

gcguuccagg agccuuugua caaaccaaag aagaacggag agcucggucc caucauucgg     2700

acugucaaaa ucauagauac aaagaaucag gugaucccuc ugaacgacgg caaaaccgua     2760

gccuauaacu cgaauauugu gaggguugau gucuuugaaa aggacgggaa auacuacugu     2820

gugccagugu auacaaugga uaucaugaag aaaguccuac ccaacaaggc uauugagccg     2880

aauaagccuu acagcgaaug gaaagagaug acugaagacu auaccuuccg cuuuucccug     2940

uacccaaacg aucucaucag aauagagcug cccagggaga agacgguuaa aaccacagca     3000

ggacacgaaa uuaauaucaa ggacguguuc guguauuaca aaacuaucga uucagccacc     3060

ggcggucuug aguugauuag ucaugacaac ucuuuuagcc ugcgggggau cggcucccgu     3120

acauuaaagc gcuucgaaaa guaucaagua gacguccucg gaaauauuua caaagugcga     3180

ggcgagaaga gaguugggcu ggcuucaagc gcccacccug agacuaggga aaccauacgg     3240

ccccuucagu cuacgcgcga uuga                                            3264


<210>  2729
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2729
augaagaaac uggugcucgg ccuugacauc ggggucgcca gcguuggaug ggguauuaua       60

gaugcugaga acggcgacgu gguagauacc ggggugagac uguucuccuc uagggacgca      120

ucagaaaaug agagucggcg cacaagccga cagucccgua gauugucuag gcggaagaaa      180

caccgcagag aaaggaucaa gcaauuacug cuagcccaug auauggacau uugcgauauc      240

gucaacgaga aucccuacga acuccgggug aaaggacuga acgagaaacu ugagguuucg      300

gaaauuuaug uggcgcugac ucacuuggcu aagcgccgag gcgucucaua ccucgacgau      360

gugguagagg aaggugggac gaucaaaagc gagcaucuga auaagaacau ggaggaaaua      420

aaaaguggaa agaugccuug ugagauccag cuggaaagau uugagaaaua uggccagguu      480

aggggcauga uugaaaucaa ucaggacacc gaggaguccu cuuuccucgu gaacauuuuu      540

cggacaagcg auuacaucaa ggaagccaag acuauauuag caacccaaca gaaauuccac      600

aagcagauua caaaugaguu uaucgacgaa uauauucaua ucauggccuc caaacgugau      660

uacuucaucg ggccaggaaa cgagaaguca cgcaccgacu auggugucua cagaacuaau      720

ggcgacacau gggauaaccu guuugaaauu cuuauaggga aaugcagugu guucccggag      780

gagaccaggg cugcacggag cucuuacgug gcccaggaau uuaauuugcu gaacgaccua      840

aauaaccuca agaucuccaa ccuggaguca ggaaaacuua gcgcuaguca aaagcacgau      900

auugucgaaa agguuaaaaa uucuaagaaa uucggcacga aggaccuguu gaaauauauc      960

gccuccgugu gugagugcaa gauagaagau auuggugggu gucgaguaga caagaaccgc     1020

aaacccauca ugcacacuuu ugagaauuac agaaaggugc auaacgcgcu caaugaggca     1080

ggagucgauc ugagguuaag cgaaaccgac uaugaugagc ugaugaaaau caugacacuc     1140

aacucggaaa cucuugagau uaagaaacag ugcaaggaga agcugccuca guugaccgaa     1200

gacuaccuaa augugugucu gguucucaac aaaaagggcg ccuucucugg cuggcacgcu     1260

cugucccuua aagccaugaa gcugaugaug ccagaguugu augggacauc aaagaaucag     1320

augcaaauca uucauaccau gcagcucgga aaggcauacc ugaagcccuu aaaccagcaa     1380

aaauaucuga auagugaagc uauacuagag gaaaucuaca acccuauugc cgcgcggagc     1440

auccgccaga cgaucaaggu gacuaaugcc auucucaaaa aguacgguca ccuggauucc     1500

gucauaaucg agaugccaag agacuaucag acaucugagg acgaaaggaa aaugauugag     1560

aagaugcaga aagaaaacga gaaggaaaag aaagcagcuc uuaagcgugc ccgggaggag     1620

uaccccuuuc cugaugaagc auuccgaaau cacaaagagu uggccaccaa gcugagacuc     1680

ugguaucaac aggaccaguu cugcguguac agcggcagga aaaucucagu aguugaucug     1740

gugcgcaacc caaaccaguu ugaaauugac cauaucauac cgcaaagugu cucccuugau     1800

gacagccuga auaacaaggu guuguguuuc ucugaggaaa aucagaagaa agggcagcag     1860

acucccuuuu auuaccacaa gggaaaauca ggcaacugga cauaugagca auacaaacuc     1920

uucgucgaga aacuguuuga uaagaagaaa aucaguaaua agaaacggga auuacugcua     1980

uucgaggaag acauuaacaa gugggaugug agaagggguu uuaucgcacg caaucucguu     2040

gacacccgau acgccagccg ggugauucug aacuccuauc agcaguacuu cgggaaucau     2100

gagaugggca cgaaaguaaa ggucgugcgu ggaaaguuua ccucucagcu uagaaagaag     2160

uggaggaucu ucaaagaucg caacgagucg cacagccauc acgcuauaga cgcguugauu     2220

guugcagcca cagcuaaucu gcggcucugg aagaaaaaca cucugggcgu guccgaugaa     2280

gggaaggucu augacaaaga gaccggagaa aucuuaccuu uugagaagcu gacagaauca     2340

gaguacaaug agaugaugaa caaggaacca uaucuuaauu ucaaaacuaa guugcaaaac     2400

auugagacca aagugaagua caguuauaga guaucuaaaa aggugaacag gaagaucagc     2460

gacgccacaa uauacuccac ccgagaacgc gaugguaucg acuacauugu uggcaaaauc     2520

aagaauauuu augaugucaa aacuuacaag gaccaguuuc ucaaacggua uaagaagaac     2580

aaagagcagu uccugaugua ccaacacgau ccccagacgu ucgcaauccu agaaaauaua     2640

auggccacau aucaggacgc ugagaacccu uuucuggccu acaagaaaga guauggggug     2700

auuagaaagu acagcaaaaa gggaggcggu ucaccaguga ccucucucaa guucaucgau     2760

aaaguccugg ggcagaagau cgaccuuacu gcuaaaaauc aagcggcuuu gcccaagaac     2820

aaaaagguug ugcugcaguc cauuaccccg uacagggccg auguguauua cgacgaaauc     2880

gauaagcguu aucauuuaau uggcguaaaa uacagucucu uuagcuucuc uaagcaggga     2940

uaugucauaa auccuaucgc auaccagaaa cugcuugaga aggaacaaau ucacuccgag     3000

augaaauuuu gcuucucacu guaucgcgaa gacaucauug aguuuggcga ggaucccgac     3060

aguuugacac gguacagauu cuggagcaag aacgaaucgg ggaaaaauaa gaucgaggug     3120

aagccaauag aaaaaccuaa cuuugagaag cagaccguuc ucacuaucac gaaaaagauu     3180

aaaauccuga auaaguacca gacagacgug cuaggaaacu ccuucaaggc ccaggaggaa     3240

aaagauccca ggaauauuga guga                                            3264


<210>  2730
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2730
augagauaca agaucggccu ggacauuggg auaaccagcg ugggaugggc cgucaugaac       60

cucgauaucc ccaggauuga ggaccuuggu guucggaucu ucgaucgcgc ugaaaauccu      120

cagacaggcg agucccuggc auugccacga cguuuagcca gaucugcgag gcggcgccug      180

agaaggcgga aacaccgccu agaacgaauu agaaggcucg ugauccggga ggggauacug      240

acuaaggaag aacuugacaa acuguuugag gaaaagcaug agaucgaugu auggcaauug      300

cguguggaag cucucgaccg caaacugaac aaugaugagc uggccagagu ccucuuacac      360

cuggcaaaga ggcggggauu caagucaaac cgaaaaagug agcgcagcaa uaaggaaaac      420

uccacgaugc uuaaacauau ugaggaaaau agagccaucu ugucuucgua uaggaccgug      480

ggcgagauga uuguuaagga cccgaaauuu gcucugcaca agcggaacaa gggugaaaau      540

uacacaaaca cuaucgcacg cgaugaccua gagagagaga uaaggcucau uuucucaaaa      600

cagcgugaau uugggaauau gagcugcacc gaggaguucg agaacgaaua uaucacaauu      660

ugggccaguc agcggcccgu ggcuuccaag gaugacaucg agaaaaaggu cggauuuugu      720

accuucgagc cuaaagaaaa gcgagcccca aaagcgacuu acacauuuca gucuuucauc      780

gcaugggagc auauuaauaa gcugagacuu auaagccccu ccggcgccag gggccugacc      840

gaugaggaac gccgguugcu cuaugaacag gcuuuucaaa agaacaaaau cacguaccac      900

gacauuagaa cucuguuaca ucugccugac gauaccuauu ucaaggggau cguguacgac      960

aggggagagu cacgcaaaca gaaugagaac auacgauuuc ucgaacuuga ugccuaccac     1020

cagauucgga aggcaguaga caaaguuuau gguaagggca agaguagcuc uuuccugcca     1080

aucgauuucg acacauuugg guacgcuuug acucuauuca aagaugacgc cgauauccac     1140

uccuaucugc guaacgagua cgaacaaaau ggaaagagaa ugcccaaccu cgcgaauaaa     1200

guguaugaca acgagcugau ugaggaacuu cugaauuugu cauuuaccaa guucggccau     1260

cucagccuga aagccuuaag gaguauccug ccuuacaugg agcaggguga agucuauucu     1320

uccgcaugcg agcgcgcugg guacacauuu accggaccaa agaagaaaca gaagacgaug     1380

cuacucccga acauuccccc uauagccaau ccagugguga ugcgggcucu gacucaggcc     1440

agaaaagucg uuaacgcaau cauuaagaaa uacggcagcc ccgugucgau ccacaucgaa     1500

cuugccaggg auuugucuca aacauucgac gagcgacgca agaccaagaa agagcaggac     1560

gaaaaucgga agaaaaacga gacugcuauu agacagcuga uggaauaugg ccucacacug     1620

aauccuaccg ggcacgauau aguaaaguuu aaacuuuggu ccgagcaaaa cggaaggugu     1680

gcguacucac ugcagccaau cgaaauugag cguuugcucg agcccgguua uguggaaguc     1740

gaccauguga ucccguacag ucgcagccug gaugacuccu auacgaauaa gguuuuagug     1800

cugacccggg agaacagaga aaaaggcaau aggauuccug cagaguaccu aggggucgga     1860

acagaacgau ggcagcaguu cgagacuuuu gugcucacca acaagcaauu cucuaagaaa     1920

aagcgggauc gccugcuuag auugcacuau gacgagaacg aagagacaga auuuaaaaau     1980

aggaaccuga augauacucg guacaucagc cgauucuuug ccaacuucau aagagagcau     2040

cucaaguucg cugagucaga cgauaaacag aagguauaca ccguuaaugg ccgcgugaca     2100

gcccaccuga ggagucguug ggaauuuaac aagaaucggg aggaauccga cuuacaucac     2160

gcagucgaug ccgcuaucgu ggccugcacc acucccagcg acauugcaaa agucacggcg     2220

uucuaucaga gaagggagca gaacaaggaa cuggcuaaaa agacagagcc acacuuuccu     2280

caacccuggc cacauuucgc cgaugagcuu cgcgcacgau ugucuaaaca ccccaaggaa     2340

ucaaucaagg cccucaaucu ggguaacuac gacgaccaga aacuagagag ucugcagccu     2400

guguuuguua gcagaaugcc aaagcggucc gugaccgggg cugcccauca ggaaacucuc     2460

aggcgcuaug uaggcauuga ugagcguucu ggaaaaaucc aaaccgucgu gaagacaaaa     2520

cugucggaaa uaaaacuuga cgcaagcggc cacuucccga uguacgggaa ggaguccgau     2580

cccagaaccu augaggcuau ucggcagagg uugcuggaac acaauaacga cccuaaaaag     2640

gccuuucagg agccauuaua caaacccaag aaaaauggag aaccuggucc aguuauccgc     2700

acugugaaga uuaucgauac aaagaaccag gucauacccc ucaaugacgg caagacggug     2760

gcguauaacu caaacaucgu acgaguggau guuuucgaga aggacgggaa auacuacugu     2820

gucccggugu auaccaugga uauuaugaag ggaauccugc cuaauaaagc cauugaacca     2880

aacaagcccu acagugagug gaaagagaug acugaagacu auacauuuag auucucucuu     2940

uacccuaaug aucugauccg gauagaguug cccagggaaa agaccgugaa gacagcugca     3000

ggcgaggaga uuaacgucaa agacguuuuu guguauuaca agacuaucga uagcgccaau     3060

ggugggcucg aacugaucuc ccaugaccac cgcuucucac uaagaggcgu gggaagcagg     3120

acccugaaac gguuugagaa guaucaagua gacguccucg gcaacauuua caaagugcgu     3180

ggggaaaagc gcguuggacu ggcuucuucc gcccauagua agacggguga gaccgugcga     3240

ccacuucaga gcacaagaga uuga                                            3264


<210>  2731
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2731
augagauaca agaucggccu ggacauuggg auaaccagcg ugggaugggc cgucaugaac       60

cucgauaucc ccaggauuga ggaccuuggu guucggaucu ucgaucgcgc ugaaaauccu      120

cagacaggcg agucccuggc auugccacga cguuuagcca gaucugcgag gcggcgccug      180

agaaggcgga aacaccgccu agaacgaauu agaaggcucg ugauccggga ggggauacug      240

acuaaggaag aacuugacaa acuguuugag gaaaagcaug agaucgaugu auggcaauug      300

cguguggaag cucucgaccg caaacugaac aaugaugagc uggccagagu ccucuuacac      360

cuggcaaaga ggcggggauu caagucaaac cgaaaaagug agcgcagcaa uaaggaaaac      420

uccacgaugc uuaaacauau ugaggaaaau agagccaucu ugucuucgua uaggaccgug      480

ggcgagauga uuguuaagga cccgaaauuu gcucugcaca agcggaacaa gggugaaaau      540

uacacaaaca cuaucgcacg cgaugaccua gagagagaga uaaggcucau uuucucaaaa      600

cagcgugaau uugggaauau gagcugcacc gaggaguucg agaacgaaua uaucacaauu      660

ugggccaguc agcggcccgu ggcuuccaag gaugacaucg agaaaaaggu cggauuuugu      720

accuucgagc cuaaagaaaa gcgagcccca aaagcgacuu acacauuuca gucuuucauc      780

gcaugggagc auauuaauaa gcugagacuu auaagccccu ccggcgccag gggccugacc      840

gaugaggaac gccgguugcu cuaugaacag gcuuuucaaa agaacaaaau cacguaccac      900

gacauuagaa cucuguuaca ucugccugac gauaccuauu ucaaggggau cguguacgac      960

aggggagagu cacgcaaaca gaaugagaac auacgauuuc ucgaacuuga ugccuaccac     1020

cagauucgga aggcaguaga caaaguuuau gguaagggca agaguagcuc uuuccugcca     1080

aucgauuucg acacauuugg guacgcuuug acucuauuca aagaugacgc cgauauccac     1140

uccuaucugc guaacgagua cgaacaaaau ggaaagagaa ugcccaaccu cgcgaauaaa     1200

guguaugaca acgagcugau ugaggaacuu cugaauuugu cauuuaccaa guucggccau     1260

cucagccuga aagccuuaag gaguauccug ccuuacaugg agcaggguga agucuauucu     1320

uccgcaugcg agcgcgcugg guacacauuu accggaccaa agaagaaaca gaagacgaug     1380

cuacucccga acauuccccc uauagccaau ccagugguga ugcgggcucu gacucaggcc     1440

agaaaagucg uuaacgcaau cauuaagaaa uacggcagcc ccgugucgau ccacaucgaa     1500

cuugccaggg auuugucuca aacauucgac gagcgacgca agaccaagaa agagcaggac     1560

gaaaaucgga agaaaaacga gacugcuauu agacagcuga uggaauaugg ccucacacug     1620

aauccuaccg ggcacgauau aguaaaguuu aaacuuuggu ccgagcaaaa cggaaggugu     1680

gcguacucac ugcagccaau cgaaauugag cguuugcucg agcccgguua uguggaaguc     1740

gaccauguga ucccguacag ucgcagccug gaugacuccu auacgaauaa gguuuuagug     1800

cugacccggg agaacagaga aaaaggcaau aggauuccug cagaguaccu aggggucgga     1860

acagaacgau ggcagcaguu cgagacuuuu gugcucacca acaagcaauu cucuaagaaa     1920

aagcgggauc gccugcuuag auugcacuau gacgagaacg aagagacaga auuuaaaaau     1980

aggaaccuga augauacucg guacaucagc cgauucuuug ccaacuucau aagagagcau     2040

cucaaguucg cugagucaga cgauaaacag aagguauaca ccguuaaugg ccgcgugaca     2100

gcccaccuga ggagucguug ggaauuuaac aagaaucggg aggaauccga cuuacaucac     2160

gcagucgaug ccgugaucgu cgcuugcacc acucccagcg acauugccaa agugacggca     2220

uucuaucaga gaagggagca gaacaaggaa cuggcgaaaa agacagagcc acacuuuccu     2280

caacccuggc cacauuucgc ugaugagcuu cgcgcccgau ugucuaaaca ccccaaggaa     2340

ucaaucaagg cacucaaucu ggguaacuac gacgaccaga aacuagagag ucugcagccu     2400

guuuuuguga gcagaaugcc aaagcggucc guaaccgggg ccgcucauca ggaaacucuc     2460

aggcgcuaug ucggcauuga ugagcguucu ggaaaaaucc aaaccguggu uaagacaaaa     2520

cugucggaaa uaaaacuuga cgccagcggc cacuucccga uguacgggaa ggaguccgau     2580

cccagaaccu augaggcaau ucggcagagg uugcuggaac acaauaacga cccuaaaaag     2640

gcuuuucagg agccauuaua caaacccaag aaaaauggag aaccuggucc agugauccgc     2700

acugucaaga uuaucgauac aaagaaccag gugauacccc ucaaugacgg caagacggua     2760

gccuauaacu caaacaucgu gcgaguugau gucuucgaga aggacgggaa auacuacugu     2820

gugccggugu auaccaugga uauuaugaag ggaauccugc cuaauaaagc gauugaacca     2880

aacaagcccu acagugagug gaaagagaug acugaagacu auacauuuag auucucucuu     2940

uacccuaaug aucugauccg gauagaguug cccagggaaa agaccgucaa gacagccgcu     3000

ggcgaggaga uuaacguuaa agacguguuu guguauuaca agacuaucga uagcgcaaau     3060

ggugggcucg aacugaucuc ccaugaccac cgcuucucac uaagaggcgu aggaagcagg     3120

acccugaaac gguuugagaa guaucaaguc gacgugcucg gcaacauuua caaaguucgu     3180

ggggaaaagc gcgugggacu ggccucuucc gcucauagua agccagguaa aacgauccga     3240

ccucuucaga gcaccagaga uuga                                            3264


<210>  2732
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2732
augagauaca agaucggccu ggacauuggg auaaccagcg ugggaugggc cgucaucaac       60

cucgauauuc ccaggaucga ggaccuuggu guucggauuu ucgaucgcgc ugaaaauccu      120

cagacaggcg agucccuggc auugccacga cguuuagcca gaucugcgag gcggcgccug      180

agaaggcgga aacaccgccu agaacgaauc agaaggcuca uaauccggga ggggauucug      240

acuaaggagg aacuugacaa acuguuugag gaaaagcaug agaucgaugu guggcaauug      300

cguguagaag cucucgaccg caaacugaac aaugaugagc uggccagagu gcucuuacac      360

cuggcaaaga ggcggggauu caagucaaac cgaaaaagug agcgcagcaa uaaggaaaac      420

uccacgaugc uuaaacauau ugaggaaaau agagccaucu ugucuggcua uaggaccguc      480

ggugagauga uagugaagga cccgaaauuu gcucugcaca agcggaacaa gggggaaaau      540

uacacaaaca cuauugcacg cgaugaccua gagcaugaga ucagacucau uuucucgaaa      600

cagagggaau uuggaaauau gucaugcacc gagaaguucg aaaacgagua uaucacaauc      660

ugggccagcc agcgucccgu ugcuaguaaa gaugacauug aaaagaaagu gggcuuuugu      720

accuucgagc cuaaggagaa acgggcccca aaggcgacuu acacauuuca guccuucaua      780

gcaugggaac acaucaauaa gcugcgacuu auuuuucccu cuggcgccag agggcugacc      840

gaugaggaaa ggcgcuugcu cuaugagcaa gccuuccaga aaaacaagau cacguaccac      900

gacauacgga cucuguuaca ucugccugac gauaccuauu uuaaaggaau ugucuacgac      960

agaggugaaa gcaggaagca gaaugagaac auccgcuucc ucgagcuuga ugcuuaccac     1020

cagauccgaa aagcagugga caagguauau ggcaagggga aauccucaag uuuccugcca     1080

auugauuuug acacauucgg auacgcuuug acucuauuua aggaugacgc cgauauccau     1140

agcuaucugc ggaacgaaua cgagcaaaau ggcaaacgua ugcccaaccu cgcgaauaag     1200

guuuaugaca acgaacugau ugaggagcuu cugaauuugu cuuucaccaa auuuggucac     1260

cucucccuga aggccuuaag aucaauacug ccuuacaugg aacaggggga gguguauagc     1320

agugcaugcg aaagggcugg auacacauuc accggcccaa agaaaaagca gaaaacgaug     1380

cuacucccga acauuccccc uaucgccaau ccagucguga ugcgcgcucu gacucaggcc     1440

cggaaggugg ucaacgcaau caucaaaaag uacggcucuc ccguuuccau ucacauagag     1500

cuugccagag auuugagcca aacauuugac gaaaggcgaa agaccaaaaa ggagcaggac     1560

gagaaucgca aaaagaacga aacugcuauc cggcagcuga uggaguaugg gcucacacug     1620

aauccuaccg gacaugauau ugugaaauuc aaacuuuggu cggaacaaaa cgguagaugu     1680

gcguacucuc ugcagccaau cgagauugaa agguugcucg agcccggcua uguagaggug     1740

gaccacguca ucccguacuc ccguucacug gaugacaguu auacgaauaa aguguuaguu     1800

cugacccgcg aaaaccggga gaaggggaau agaauaccug cagaauaccu aggagugggc     1860

acagagaggu ggcagcaguu ugaaacuuuc guccucacca acaagcaauu uagcaaaaag     1920

aaacgagauc ggcugcuucg cuugcauuau gacgagaacg aggaaacaga guucaagaau     1980

agaaaccuga augauacuag guacaucucc cgguuuuucg ccaacuucau ucgagaacac     2040

cucaaauuug cugagucuga cgauaagcag aagguguaca ccguaaaugg uagaguuaca     2100

agccaucugc gcucaaggug ggacuucaac aaaaaucgug aggaaaguga uuuacaccac     2160

gccguggacg cagccaucgu cgcuugcacc acucccuccg auauugccaa ggugacggca     2220

uuuuaucagc ggagagagca gaacaaagaa cuggcgaaga aaacagagcc acauuucccu     2280

caacccuggc cacacuuugc ugacgaacuu agggcccgcu ugagcaagca ucccaaggag     2340

ucuaucaaag cacucaaucu ggggaacuac gacgaucaga agcuagaguc acugcagccu     2400

gucuucguga gucgaaugcc aaaaagaagc guuaccggcg ccgcucacca ggaaacucuc     2460

cggagguaua uaggaauuga cgagcgcucc ggcaagaucc aaaccguggu aaaaacaaag     2520

cugucugaaa uuaaacuuga ugccucgggg cacuuuccga uguacggaaa ggagagcgac     2580

ccccguaccu augaagcaau cagacagcgg uugcuggagc auaauaacga uccuaagaag     2640

gcuuuccagg agccauuaua caagcccaaa aagaauggug aaccuggccc aauaaucagg     2700

acugucaaaa uuaucgacac aaagaaccag gugauucccc ucaaugaugg gaagacgguu     2760

gccuauaacu ccaacaucgu gcgcgucgac guguuugaga aagauggaaa guacuacugu     2820

guaccggugu auaccaugga cauaaugaaa ggcauucugc cuaauaaggc gaucgaacca     2880

aacaaacccu acucagagug gaaggaaaug acugaggauu auacauuccg auuuagucuu     2940

uacccuaaug accugaucag aauugaguug ccccgggaaa agaccguuaa aacaacuacc     3000

ggugaggaaa ucaacgucaa ggauguguuc guguauuaca aaacgauuga cucugccacc     3060

gggggccucg agcugauaag ccacgacaau agguuuuccc uacgcggagu cggcucaaga     3120

acacugaaga gguucgagaa auaucaaguu gaugugcucg ggaacaucua caaggugcgg     3180

ggagaaaagc guguaggucu ggcuagcucu gcacauucca aaacuggcga gaccauucgc     3240

ccacuucaga guacacgaga cuga                                            3264


<210>  2733
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2733
augagauaca agaucggccu ggacauuggg auaaccagcg ugggaugggc cgucaucaac       60

cucgauauuc ccaggaucga ggaccuuggu guucggauuu ucgaucgcgc ugaaaauccu      120

cagacaggcg agucccuggc auugccacga cguuuagcca gaucugcgag gcggcgccug      180

agaaggcgga aacaccgccu agaacgaauc agaaggcucc ugauacggga gggggugcuu      240

acuaaggaag aacuggacaa auuguuugag gaaaagcaug agaucgaugu auggcaacuc      300

cguguggaag cucuggaccg caaacugaac aaugaugagc ucgucagagu guuacugcac      360

cuugccaaga ggcggggauu caagucaaac cgacagagug agcgcagcaa uaaagaaaac      420

uccacgaugu ugaagcauau ugaggaaaau agagcaaucc ugucuggcua uaggaccguu      480

ggugagauga uugugaaaga cccgaaguuu gcccuacaca aacggaacaa gggggaaaau      540

uacacaaaca cuaucgcucg cgaugaccuc gagagagaga uaaggcugau uuucucgaag      600

cagcgugaau uuggaaauau gucaugcacc gaggaguucg agaacgaaua uaucgcaauu      660

ugggccagcc agcggcccgu cgcuaguaaa gaugacaucg agaagaaagu gggcuuuugu      720

acauucgagc cuaaggaaaa acgagcccca aaggcgaccu acacuuuuca auccuucauc      780

gcaugggagc auauuaauaa acuuagacug auaucuccca gcggcacaag gggguugacc      840

gaugaggaac gccggcuccu guaugaacag gccuuucaga agaacaagau cacguaccac      900

gacauuagaa cuuuacugca ccucccugac gauaccuauu ucaaaggaau cguauacgac      960

uggggugagu cccuuaagca gaaugagaac auaagguuuc uggaauugga ugcuuaccau     1020

caaauucgca aagccaucga caagguuuau ggcaaaggga agucaaguag cuuccuacca     1080

aucgauuucg acacauuugg auacgcacug acucucuuca aggaugacgc ugauauucga     1140

ucuuaucugc ggaacgagua cgaacagaau ggcaaacgua ucgccaaccu ugcgaauaag     1200

guguaugaca acgagcugau ugaggaauug cucaaucugu ccuuuaccaa auucggucac     1260

uuaucacuga aggcccuaag aaacauacuc cccuacaugg agcaggggga agucuauagc     1320

agugcaugcg agaaagcugg auacacauuu accggcccua agaagaaaca gaagacggug     1380

cugcuuccaa cuaucccgcc cauugugaau ccugucguua ugagggccuu gacacaagcu     1440

cgcaaagugg uaaacgccau caucaagaaa uacggcucuc caguguccau ucauauagaa     1500

cuggcacggg aucucagcca gaccuucgac gagagaagga agacuaagcg agagcaggac     1560

gaaaaucgca aacggaacga gacagccauc agacaacuga uggaauaugg gcuuacccug     1620

aaucccacgg gauuggauau ugucaaguuu aaacucuggu cggagcagaa cgguaggugu     1680

gcuuacucuc ugcagccuau cgaaauugag cguuuacugg agccaggcua uguggaaguu     1740

gaccacguga uccccuacuc ccgcucacua gaugacaguu auaccaauaa gguccucgug     1800

cugacaaaag agaaccggga aaaggggaau agaauaccgg cggaguaccu uggaguaggc     1860

acugaaaggu ggcagcaauu cgagaccuuu guuuugacaa acaaacaguu cagcaagaag     1920

aaacgagauc ggcugcuccg ccugcacuau gacgagaacg aagagaugga auuuaagucc     1980

agaaauuuaa acgauacuag guacaucucu cgguucuuug caaauuucau ucgagagcau     2040

cugaaauucg ccgagagcga cgauaagcag aaaguguaca ccgucaacgg uagagugaca     2100

ucacaccuuc gcaguaggug ggacuuuaau aagaaccgug aagaguccga uuugcaucac     2160

gcugucgacg ccgcaaucgu ggccugcacc acuccuagcg auauugcuaa gguuacggcc     2220

uucuaucagc ggagagaaca aaauaaagag cucgcaaaga aaacagaacc ccaguuucca     2280

cagccuuggc cccauuucgc ggacgagcug agggcucgcc uaucuaagca cccaaaagag     2340

ucaaucaagg cccugaaccu cgggaauuac gacgaucaga agcuggaaag ucuucaaccc     2400

guguuuguaa gccgaaugcc uaaaagaucc gucaccggcg cagcccacca ggagacuuug     2460

cggagguaua uaggaauuga cgaacgcucu ggcaagaucc agaccguggu uaaaacaaag     2520

cugucggaga uuaaauuaga ugcuagcggg cauuucccaa uguacggaaa ggaauccgac     2580

ccgcguaccu augaggccau cagacagcgg cuccuggagc acaacaauga ucccaagaaa     2640

gcauuucaag aaccucuuua caagcccaaa aagaacggug agccaggccc uauaaucagg     2700

acugugaaaa uuaucgacac aaagaaucag gucauuccac ugaacgaugg gaaaacggug     2760

gcuuauaauu caaacaucgu acgcauagac guguucgaaa aggauggaaa guacuacugu     2820

guucccgucu auaccaugga cauuaugaaa ggcguguugc cgaacaaggc caucgagccu     2880

aauaaaccau acagugaaug gaaggagaug acugaggauu auacauuucg auucucucuc     2940

uaccccaacg accugaucag aauugaacua ccucgggaga aaaccgugaa gacagcggcc     3000

ggugaagaga ucaauauuaa ggauauguuu gucuauuaca aaacuauaga cagcgcuaac     3060

gggggccugg agcucaucuc ccaugacaau ucauucagcc ugaggggagu uggcucucgc     3120

acccuuaaga gauuugaaaa auaucaggug gauguguugg ggaacauuua caagguaagg     3180

ggagagaaac gggucggucu ggcauccagu gcccacagca agacgggcga aaccauccgu     3240

cccuuacaau cuacacgcga cuga                                            3264


<210>  2734
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2734
augcccaagg aguucgaccc ucugaaacca gccaaguaca gacucgcucu ugauaucggc       60

agcaaaucca uuggguuugc aauaaucuuc cugaaggaag acgugaacaa auuguuaaag      120

ccguaugcca uugucagguc uggaguucgg aucuuuggug auggccgcac ccccgcggcu      180

ccugggcagc ugggaucaag ucuagccgca gagcgacgug ugaagagaag cauuaggcgg      240

caauacgccc gcaaaagaaa gaggcuccag cggcugcuuc gcaaucugaa agaauugggc      300

uucuuuccag acgauuccaa gcucugcaaa ucuuucgagg accugaaccc cuaugaacug      360

cgacacaagg gucucacaaa ggaguuaaaa ccucaugagu uugggagacu gcuuaugcac      420

auggcuaaga auaggggauu ccagucgaac cggaaaguag auuugcuggc agacaauauc      480

gauucacagg ccaaggcuga aacuagcgcc augaaaagug cgauagcaac gcuacaaucc      540

cgucucaacg gcgaguuuga aacccugggc gccuaccuuu augagcgcuu caagaaaggg      600

gagacaauca agucuagcaa gacucagaaa ggacagaagg cugaauacga guuuuccauu      660

ucaagaagga uggugaaaca ggaguucgac cggcuguggg ccgcacaaga gcaguuuaau      720

ccagaauugu ucaacgagca ggcuaagaau acccucaaag auacaaucuu cuuucagcga      780

aaccuguuac cccaaaaggu cgguaagugu accuuccugc cugagcauga acgcgccagu      840

aaagcgcucc caagcucuca guauuuuaga auuaucuccg agcuuaauaa gauaucauuc      900

acugacgccg aaggccugau uuugccgauc agccuagagc agaggaacaa acugcucaag      960

agucugcgga uuaaacaaaa acuuucuuuu gaucgcauca gaacagaacu gaauuuggac     1020

uccaccgugc ucaucaaccu ggagcacgca agcaaggacu cgauugaggg ggauaagacg     1080

ggauacuuac ugucuaagaa agaauacuuc ggccccgcuu ggagggaccu aucccucgag     1140

acucaggaug ccauaguuac cgcucugcuu acacaugaaa augaggccga auuggugcag     1200

aagcuggucg acgauuuuaa caucgcacac gacguggcca aagcucucgc gaacgcacgu     1260

cuggagucac aguauaugcg gcuuaguaag gaggccauug aacgacugau cccugaguug     1320

gaaaagggug uagauagcca agacggguac aaaguuccag ugaaguauca cgaggcuacu     1380

gaggccgcag gauucggcca ucacuccgcc cuccaggaaa auugggcuga uuuucccacc     1440

cuggacuuaa aacagcugca uucugaugac aacgacaagu acacagucgc cgagcuaccu     1500

cucccauauu acggcaaagu gauaaccaga cagguggcau ucgggacggg agaugucaau     1560

gaccccguug aaaagagguu ugguaaaauu gcgaacccga cugugcacau cugccugaau     1620

caaguacgcg ugcuuuugga ugagaucauu gcuaaguaug gcuacccuua cgaagucaac     1680

aucgagcugg cccgggagcu caagcagaau cacgaacaga gacagaggag cauuaaacaa     1740

caggcagcca acaagaaaga gaaugaagag auacgccgag aaaucaagca ggcuuauaaa     1800

guggagaagg cccggauuau cacagacuca gagaucaguc gugcagauau ugaaaagugg     1860

agacugugga ggcgcuccaa aagcaaguua uguguuuacu cugggaaaaa gauaugcgag     1920

accaugaucc ugaacaauga aguggagguc gaccauauuc uuccccgguc aagaacugga     1980

gauaacaguu aucagaauaa aguguuggua cucaacaagg cuaauaggau caagagcaac     2040

cgaacaccau gggaagccaa agagcuguuc gaggcgcaag gcugguccua cgaagacauu     2100

cuaaaccgca ugcgggcccu ggauaagcac cagcucuuua gauucgcuga aaauucuaug     2160

gacaucuggg auaaaaacua uaggucguuc gaagcacgug cccugaauga cacccgcuac     2220

auaagccggg uugugaagga uuaucuuaaa gucguguguc auaaggacag aaucgucgug     2280

uccaacggug cucacacggc cuugauuagg cgagaguuug ggcugaauga cuuacucggc     2340

aagaaaggag agaagaaccg ggaugaccau cgccaccacg caaucgaugc gugcguuauu     2400

ggcgcccugg acagaucaau gcuuaggaaa cugcagaucg cuauggaagu ggccgagaag     2460

caaaauagug cacggaacuu cguaggguug caggaaccuu gguuuaauuu cucuacccag     2520

guccgaagaa gcauaggaca gauuuacgug ucccauaaac ccaaccacaa uuacgagggu     2580

gaaaucuuug aggcuucaau uuauggccuc cgccugauca gcgauaagag gacagagaaa     2640

uacguuggga uaaagcguaa cccagaaacu ggaaagaccg acaaacccaa gaaaucugug     2700

gucgugaucu ccgauccuaa gacacaaaau cgguauggcc uaagacauaa auuccacagu     2760

gagggucugc caaagaagua cuaugggcuc ucuagcuccu caguauuuug ucuggaaauu     2820

uacagggacg agaaaggcaa cuggcaggcc uauacuaucc cgcgcuacga agcauaccga     2880

auugugaaga gacggggagu uaaugcccuu uaugauaaca aaaggaguaa cuucgagggc     2940

cgccccuugg ucaugcgucu gaugagagac gauugcaucg cuuuugagaa gaauggggug     3000

aaagaagugu accggauaca gaagauuaac ggauuaaaga aaaauaucac ccucacaagg     3060

cacuucgacg cgaacgucga ugaccgcuau cgaagaaugc ugaaggccca gaaagcagag     3120

aaggcuggug aagacuuuga uagcgaggac cuuugugaug accugguuuu cucguccuuu     3180

ggcgccucua gccuauacuc aaauaaagcc cggaagguga ccaucagucc uaugggggug     3240

cucagggauc caggauucaa gggcuga                                         3267


<210>  2735
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2735
augaagaaac uggugcucgg gcuugacauc ggggucgcca gcguuggaug ggguauuaua       60

gaugcugaga acggcgacgu gcuggaugca gggguaagau uguucuccuc uagggacgcc      120

ucagaaaaug agagucggcg caccagccga cgucagucca gaagguuauc ucggcgcaag      180

aaacacagaa gggaacggau caagcaacug cuacucgcgc augauaugga cgugugcgau      240

auugucaacg agaaucccua ccagcugcgc gugaaaggac uuaacgaaaa gcuggagauc      300

ucggagauuu auguugcuuu gacacaccuc gccaagcgaa gaggcguguc auaccuggac      360

gaugucgugg aagagggugg ggcaaucaaa agcgaacauc ugaauaagaa cauggaggag      420

auaaaaagug gaaagaugcc uugugaaauc cagcucgaga gguuugaaaa auauggccag      480

guacggggca ugauugagau caaucaagac acugaagagu ccucuuucuu aguuaacauu      540

uuucguacga gcgauuacau caaggaggcc aagaccauac uggcuacaca gcagaaauuc      600

cacaagcaga uuacuaauga auuuaucgac gaguauauua acaucauggc auccaaacgc      660

gauuacuuca ucgggccagg aaaugaaaag ucaagaaccg acuauggugu guacaggaca      720

aacggcgaca ccugggauaa ucuuuuugag auuuugauag ggaaaugcag ugucuucccg      780

gaagagacuc gggccgcucg aagcucuuac guggcccaag aguuuaaccu gcuaaaugac      840

cucaacaacc ugaagaucuc cucacuugaa agcggaaaac ugaguacauc ucagaagcau      900

gauauugugg agaaggucaa aaauuccaag aaauucggca ccaaggacuu gcucaaauau      960

aucgcgagcg uuugugaaug caagauagag aacauugaug guugucgcaa ggacaaaaau     1020

agaaagccca ucaugcacac guuugaaaac uacaggaaag ugcacaaugc acugaacgag     1080

gccgggguag auuuacggcu gucggaggcu gacuaugaug aacucaugaa gaucaugacu     1140

cuuaauucug agacccugga aauuaaaaag cagugcaagg agaaauugcc ucaucuaaca     1200

gaggacuacc ugaacgugug ucucguccug aauaagaaag gagccuucuc cggcuggcac     1260

gcacuuucac ugaaggcuau gaaauugaug augccagaac ucuauggcac uucuaagaac     1320

cagaugcaaa ucauucauac caugcagcug gggaaggccu acauaaaacc cuuaaaucag     1380

caaaaguauc ugaacagcga ggcgauccua gaagagauuu acaauccuau cgccgcacgc     1440

uccaucagac agacaauuaa agugaccaac gcuauacuca agaaauacgg accacuggau     1500

ucuguuauca uugaaaugcc cagggacuau cagacgagcg aggacgagcg uaagaugauc     1560

gagaaaaugc agaaggaaaa ugaaaaggag aaaaaggccg cucuuaaacg ggcccgagaa     1620

gaguacccuu uuccagauga ggcauucaga aaccacaagg aauuggccac uaaacugagg     1680

cucugguauc aacaggacca guucugcgug uacucagguc gcaagauuag ugucguggau     1740

cugguacgga acccgaauca guuugagauc gaccacauaa ucccccaauc cguuagccuu     1800

gaugacucuc ugaacaauaa gguguugugu uucucagaag agaaccagaa aaagggccag     1860

cagacaccuu uuuauuacca uaaagggaag aguggaaauu ggaccuauga acaauacaaa     1920

cucuucgucg agaagcuguu ugauaagaaa aagauuagca acaaaaagag agaguuacug     1980

cuauucgaag aggacaucaa uaaaugggau gugaggcgcg gcuuuauugc ucgaaaccuc     2040

gucgacacuc gguacgcguc ccgugugauc cugaauaguu aucagcagua cuucgguaac     2100

cacgaaaugg ggacaaaggu uaagguggua agaggcaaau uuaccucgca gcuuaggaag     2160

aaauggaaga uauucaaaga ucgcaaugag agccauuccc accaugcaau ugacgccuug     2220

aucgucgcug ccacggcaaa ccugcggcuc uggaagaaaa auaagcuggg aauuucagau     2280

gagggcaagg uguaugacaa agaaaccggg gagaucuuac cauuugaaaa gcugacagcc     2340

agugaguaca acgaaaugau gaauaaagag cccuaucuua acuucaagac uaaauugcaa     2400

aacauagaga ccaagguuaa guacucuuau aaagugagca agaaagucaa uagaaagauc     2460

uccaacgaca caauuuacuc aacuagggaa cgagauggaa ucgacuacau uguggguaaa     2520

aucaagaaua uauaugaugu aaagaccuac aaagaccagu uucucaagcg cuauaaaaag     2580

aacaaagagc aguuccugau guaccaacac gauccucaga cauucgcuau ucuagaaagc     2640

aucauggcca ccuauccaga cgcagagaau cccuuucugg cguacaagaa ggaacacggc     2700

gugauccgga aauaugcuaa gaacaaagcc ggggcaccgg uuacuucucu caaguucauu     2760

gauggagccc ugggccagaa aaucgacguc acguacaaga auaaggcugc ccuuccuaaa     2820

aacaagaaag ugguguugca guccauuaag cccuacagag cagaugucua uuacgacgag     2880

gguaauaaaa gguaucaucu gauagggguu aaguacaguu uauuuagcuu cucuaaacaa     2940

ggcuauguga ucaacccaau ugcuuaccag aagcuccugg agaaggaaca gauccacucc     3000

gagaugaaau uuugcuucuc acuuuaucgu gaagauauua ucgagagugg agaagacccu     3060

gauagccuga cacgcuaccg guuuuggucg aagaaugagu ccggcaaaaa caagauagag     3120

gugaaaccca ucgaaaagcc aaauuucgag gacagaaagg uaauuaccau cacuaaaaag     3180

auuaaaaucu ugaacaagua ccagaccgac guccucggca auucuuuuaa agcccaagaa     3240

gagaaggauc ccaggagcau agaguga                                         3267


<210>  2736
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2736
augaacuaca agaugggccu ggacaucggg auugccagcg ugggaugggc ugucauaaau       60

cucgaucuua aaagaaucga ggaccugggu guuaggauuu ucgauaaggc agaacacccc      120

cagaacggcg aguccuuggc cuuaccucgg cgcaucgcgc gaucugcucg uagaaggcug      180

cggcgcagaa aacauaggcu agaacggauu cgccgacucc uggugucaga gaauguacuu      240

accaaggaag agaugaaccu guuguuuaaa caaaagaagc agaucgacgu guggcagcuc      300

agagucgaug cccuggagag gaaacugaau aacgacgaac ucgcacgggu guuacugcac      360

cuugccaagc gucgcggguu caaaaguaau agaaagagcg agaggaacuc caaagaaucu      420

ucggaguuuu ugaagaauau agaggaaaac cagucaaucc uggcucaaua ucggagcguu      480

ggagagauga uugugaagga uaguaaauuc gcauaccaua agcgaaauaa acuagacucc      540

uauucuaaca ugaucgcccg cgaugaccuc gaaagagaga uuaagcugau cuuugaaaaa      600

cagagggagu ucaauaaccc agucugcaca gagcggcuug aagagaagua ccugaauaua      660

uggagcuccc agcgcccguu ugcuucaaaa gaagauauug agaagaaggu gggcuucugu      720

acuuuugaac ccaaagagaa gagagccccu aaagcgacgu auaccuucca gaguuuuauc      780

guaugggagc acauuaacaa guugaggcuc guuagcccag acgaaacacg ugcacugacu      840

gagaucgaac ggaacuuacu guacaaacaa gccuucucua agaauaagau gaccuauuac      900

gauauccgaa aacuccuuaa ccuguccgac gacauucauu uuaaggguuu gcuauacgau      960

cccaaaucaa gccugaagca gauagagaau aucagauucc ucgaacugga caguuaucac     1020

aaaauuagga agugcaucga gaacguguac gggaaggaug gaauacgcau guucaaugag     1080

acagacauug auaccuuugg cuaugcucuu acuaucuuca aagacgauga agacaucguc     1140

gccuaccugc agaacgagua uauuacaaag aauggcaaac gggugucuaa cuuggcaaau     1200

aagguguacg auaaaucccu caucgacgaa cuguuaaacc ugagcuuuuc gaaguucgcu     1260

caccuaucua ugaaggccau uagaaauaua cucccuuaua uggagcaggg ggagaucuac     1320

uccaaagcgu gugaacuggc cggauacaac uuuaccgguc caaagaaaaa ggagaaagca     1380

cuuuugcugc ccgucauucc uaauaucgcu aacccaguug ugaugagggc ccucacgcaa     1440

ucacgcaagg uagugaaugc uaucauuaaa aaguauggca guccggucag cauacauauc     1500

gaacuggccc gagaucuuuc ccacucuuuc gacgagcgga agaaaauuca gaaggaccag     1560

acugaaaacc guaaaaagaa cgagaccgca aucaaacaac ugauugagua cgaauugaca     1620

aagaauccca cugggcucga uaucgugaag uuuaaacugu ggagcgagca gcagggaaga     1680

ugcauguauu cauuaaagcc uauagaacug gagaggcuac ucgaaccagg cuacguugag     1740

guggaccaua uccugcccua uagucgcucc cuugaugaca gcuacgccaa caaagucuug     1800

gugcugacca aggagaaucg ggaaaaaggu aaccacacac cuguagagua ucucgggcug     1860

ggaucugaaa gauggaagaa guucgagaaa uuuguuuuag cuaauaagca guucagcaaa     1920

aagaaaaagc aaaaccugcu uagguugcga uacgaagaga ccgaggaaaa ggaguuuaaa     1980

gaacgcaauc ucaacgauac gcgguacauu aguaaguucu uugcgaauuu caucaaagag     2040

caccugaagu ucgcagacgg cgauggcggg cagaaagugu auacuauuaa cggaaagauc     2100

acagcccauc uaagaucaag gugggacuuu aauaaaaacc gugaggaauc cgaucugcac     2160

caugcugucg acgccgugau agucgcaugu gccacccagg guaugauuaa gaagaucacu     2220

gaguucuaca aagcucgcga acagaauaag gagucugcca aaaagaaaga accaauuuuu     2280

ccccaaccgu ggccucacuu cgcagaugag cucaaggcgc ggcugucgaa guuuccccag     2340

gagagcaucg aggcuuucgc ccuuggcaac uaugacagga aaaaguugga aucccugaga     2400

ccaguguuug uuucacgaau gccuaaacgg agugugacag gggcagccca ucaggaaacc     2460

uuacgcagau gcguaggaau agaugagcag ucuggcaaga uccaaacggc ugucaaaacc     2520

aagcucagcg acauuaagcu ggacaaagau ggucacuucc ccauguacca gaaggaaucc     2580

gacccaagga cauaugaggc cauccggcag cgacuucugg agcacaauaa cgaucccaaa     2640

aaggcauuuc aggaaccuuu guacaaacca aagaagaacg gggagccggg ccccgugauu     2700

agaacuguua aaaucauaga caccaagaau aaaguggucc aucucgaugg aucaaagaca     2760

guggcuuaua acagcaauau uguacgcacu gacguguucg aaaaagaugg caaguacuac     2820

uguguuccug ucuauaccau ggacaucaug aaggggacac ugccaaacaa agccaucgag     2880

gcgaauaagc ccuacucuga auggaaagag augaccgagg aauauacuuu ucaauucucc     2940

cuauuuccua acgaucuggu gaggauugug cucccacgug agaagacgau caaaacaagu     3000

accaaugaag agauuauaau caaggacauu uucgccuacu auaaaacuau cgauagcgcu     3060

accggagguc uggagcuuau uucucacgac cggaacuucu ccuugagagg cgucggguca     3120

aagacacuga agagguuuga aaaauaccag guugaugugu uaggaaauau ccauaaggug     3180

aaaggcgaga agcgcguagg ucucgcagcc cccaccaacc agaaaaaggg gaagacuguc     3240

gacagucugc aaagcguguc ggacuga                                         3267


<210>  2737
<211>  3270
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2737
augaccgccu ucaagcccaa cccucugaau uacauccucg gccuugacau ugggauagcu       60

agcgugggau gggcaauggu cgagaucgau gacaaagaaa acccaguuag acugauugau      120

uuggguguga ggguauuuga gcgggccgaa augccgaaga caggcgacuc cuuagcggcu      180

gcccgccgac uggcacguuc ugugagaagg cuaacucggc gcagagccca caggcuccug      240

cgggcucgcc gacuucugaa acaugagggg gucuugaagc ccgaagauuu cgacgagaau      300

ggacucguga aaccucugcc auauaucccc aagcagccua gaaacacgcc auggcaacug      360

agggcagccg cucucgaucg gaaguuaaca ccccuggagu gggccgcggu ucuuuugcac      420

cuggugaaac aucguggcua ccuaucacag cgcaagaaug aaggugagac cgacgauaaa      480

gaacucgggg cacugcuuaa gggagucgcc gacaacacuc acgcucugca ggccggcaau      540

uuuagaaccc cugcagaguu ggcucucaac aaauucgaga aggaaagugg ccauauuagg      600

aaucagcgag gggauuauag ccacacauuu ucccggaagg accugcaagc cgaguuagug      660

cuccuguucg aaaaacagaa ggaguuugga aacccauacg uaucugauga ccuuaaaaau      720

gguaucgaaa accuguugau gacccagcgc ccggcgcuau cgggcgacgc cauacagaag      780

augcuggggc acugcacuuu cgagcccgca gagccuaaag cugccaagaa uacauauacc      840

gcugaaagau uuaucuggcu cacgaaacug aacaaucuua ggauucugaa ccaaggaaac      900

gagcggccau ugacugaugc cgaacgcacc acacucaugg acgagcccua caagaaguca      960

aaacugacuu augcacaggc cagaaaguua cuggaucuag acgauaccgc uuucuuuaaa     1020

ggccucaggu acgguaagga caaugcggaa agcgcaacac ugauggagau gaaagccuac     1080

caugcuauca gucgugcccu ugagaaggaa ggguugaagg auaaaaaguc cccucugucu     1140

cucagcccag agcugcagga cgaaauugga accgcauucu cccuuuuuaa aacggaugag     1200

gaaaucacug gccggcugaa gggcuguuug cagcccgaga uacucgacgc ccuguuaaaa     1260

cacauuucau ucgauaaguu cguucaaauc agucugaagg cucuacgaag aauugugccg     1320

cucauggagc aggggaaaag guaugacgaa gccugcgcag agaucuacgg agaccaucac     1380

ggucgccgga acagcgaaga gacacuguau cuuccuccca ucccagcgga ugagauuaga     1440

aauccugucg uguugagggc ucugucucag gcccgcaagg ugauaaacgg cgucgggcga     1500

cgguacggau cccccgcacg uauccacauu gaaaccgcca gagagguugg caaaucauuu     1560

aaggacagga aagaaaucga gaagcgccaa gaagagaauc ggaaggauag agagaaagcu     1620

gccgcagagu uccaggaaua uuuuccaaac uucgugggug aacccaagag caaagacaua     1680

cucaagcuga gguuauaccg acagcagaau uguaaaugcc uguauagugg gaaggagauu     1740

aaccuuggcc gcuugaauga aaaaggauac guagagaucg aucaugcucu cccuuuuucu     1800

cggacuuggg acgauuccuu caacaauaag gugcuggucc uaggcagcga gaaccaaaau     1860

aaggggaaca gaacaccaua cgaauaucug aauggaaaag acaacucgag ggaguggcag     1920

gaauuuaagg cccgugugga gaccucucgc uucccgcacu ccaaaaagca gcggauccuc     1980

cugcagaaau uugaugaaga ggguuucaag gagagaaacc uuaaugacac gagguacguu     2040

aaccgauuuu uguguaaguu cguggcggau cauaugcaac ugaccggcaa agggaagcgg     2100

cgcgucuuug ccucaaaugg acagauuaca aacuuacuca gaggcuucug gggucugagg     2160

aaagugcggg cugaaaauga ccgacaccau gcacuugaug ccguaguugu ggcuugcagu     2220

acugucgcca ugcagcagag aaucacccgc uucgugaggu auaaggagau gaacgcauuu     2280

gacgggaaaa cagucgacaa ggaaacuggc gaggugcugc accaaaagac ccacuucccc     2340

cagccuuggg aguuuuucgc gcaggaaguu augauucgug uguuuggaaa accagauggc     2400

aagcccgagu uggaagaggc cgacacaccu gaaaaacucc gggcucugcu agccgagaag     2460

cugagcucca gaccagaggc aguacaugaa uacgucgcuc cccucuucgu gucuagggcc     2520

ccgaaucgca aaaugagcgg gcagggacac auggagacca uaaagucacc uaagcgacug     2580

gaugaaggua ucaguguugu gagaguccca cuuacucaau ugaaacugaa ggacuuagag     2640

aaaaugguga accgggaaag ggagcccaag cucuaugagg cacugaaagc ccgccuugaa     2700

gcucauaagg augacccugc gaaagccuuu gcagagcccu ucuacaagca ggauaaggcu     2760

ggcaaucgua cgcagcaagu aaaagccgug agaauugaac agguucagaa gacagggguc     2820

ugggugcgga accagaaugg aaucgccgac aacgcuacca uggugagggu cgauguuuuu     2880

gagaaaggcg guaaguauua ccuggugcca aucuacuccu ggcaaguggc aaaagggauu     2940

cuaccugaca aggccauaau cgcguucaag gaugaagagg aguggcagcu cauugacgau     3000

agcuuuaauu ucaaauuucc ccugcaccca aacgacuugg uagaagucau cacuaagaaa     3060

gcucgcauuu ucggcuauuu cgccucuugu caccgaggaa ccggcaauau caacauaaga     3120

auccaugacc uggauaacaa gguggggaaa aauggaauuu uagagggcau cgguguuaag     3180

acagcacucu cauuucagaa guaccagauu gacgaacugg ggaaagagau ccggccgugc     3240

aggcuuaaga aacgcccucc cgugagauga                                      3270


<210>  2738
<211>  3270
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2738
augaacguga gauacaagau cggccuggac auugggauaa ccagcgucgg augggccguu       60

gugaaucucg auauccccag gauugaggac cuugguguac ggaucuucga ucgcgcugaa      120

aacccucaga caggcgaguc ccuggcauug ccacgacguu uagccagauc ugcgaggcgg      180

cgccugagaa ggcggaaaca ccgccuagaa cgaauuagaa ggcuccugau ccgggagggg      240

auacuuauga aggaagaacu ggacaaauug uuugaggaac gucaugagau cgaugugugg      300

caacuccgcg ucgaagcucu ggacagaaag cucaauucag augagcuggc caggguguua      360

cugcaccuug caaaacggcg aggauucaag aguaaccgca agagcgagca uaauuccaga      420

gaaaacucua cuauguugaa acacauugag gaaaauaggg ccauccuguc gucauaucgg      480

acgguugcug agaugauugu gaaggacccg aaauuugcac uacauaagcg caacaaaggc      540

gaacacuaca ccaauacaau cgccagagau gaccucgaga gggagauaca gcugauuuuc      600

acuaagcagc gugaauuugg uaacaugaau ugcaccaagg aguucgaaaa cgaguauauc      660

gcuauuuggg ccagccagcg gcccuuugcg aguaaagaug acaucgaaaa gaaagucggg      720

uucuguacau uugagccuaa ggagaaacga gcaccaaagg ccaccuacac uuuccaaucc      780

uuuaucgcuu gggaacacau uaauaaacuu agacuggugu cucccgccgg aacaaggggc      840

uugaccgaug aggaacgccg gcuccuguau gagcaggcau uccagaagaa caagauaacg      900

uaccaugaca ucagaacuuu acugaggcuc ccugacgaua ccuauuuuaa aggcguaguu      960

uacgaccgca auaugccacu uaagcagaac gaaacaauuc gauuccugga guuggaugcu     1020

uaccaccaaa uccggaaagu gauagacaag gccuauggga aaggaaagag cuccucauuc     1080

cuacccauug auuuugacac uuucgguuac gcgcugaccc ucuuuaagga ugacgccgau     1140

guccguaguu aucugcagaa cgaguacgaa cagaauggca aaccuaugcc aaaccuugca     1200

aauaagacau augacaacga gcugaucgaa gaguugcuca aucugagcuu caccaaauuu     1260

gggcauuuau cucugaaggc ucuacacaac auccucccgu acauggagca gggagaagug     1320

uauuccucag ccugcgagag agcuggcuac aaauucacgg gucccaggaa gaagcagaaa     1380

acuaugcugc uuccuaauau uccaagcauc gccaaccccg uggucaugcg cgcauugaca     1440

caagcccgga agguugugaa ugcuauuauc aaaaaguacg ggaguccugu aucuauacac     1500

auugaacugg cgagagaucu cucccagacc uuugacgaga ggcgaaaaac uaagcgcgaa     1560

caagacgaga accggaagaa aaaugagaca gcaaucagac agcugaugga auauggacuu     1620

acccugaacc caacgggcca ugauaucgug aaguucaaau uguggagcga gcagaauggg     1680

aggugugccu acucgcucca gcccauugaa auagagcguc uguuagaacc gggcuauacc     1740

gaggucgacc acgugauccc uuacucucgc ucccuggaug acucauauac aaacaagguu     1800

cuagugcuca cuaaagagaa ucgggaaaag ggaaacagaa cccccgcuga guaccugggu     1860

gucggcacag aaagguggca ucaauuugag acuuucgugc uuaccaacaa acaguuuagu     1920

aagaagaaac gagaucgguu gcugcgccuc cacuaugacg aaaaugagga gacagaguuc     1980

aagaacagaa aucugaacga uaccagguac auuagccggu uuuucgccaa uuucauccga     2040

gagcauuuaa aauuugcaga auccgacgau aagcagaaag uauacacugu uaacgggaga     2100

auuacggccc aucugcgcuc uaggugggag uucaauaaga accgugagga aagcgaccuu     2160

caccacgcug uggaugccgc aaucgucgcg ugcacaaccc caucagacau agcuaagauc     2220

acugccuuuu aucagcggag agagcaaaau aaagaauugg caaagaaaac cgagccucac     2280

uucccccagc cauggcccca uuuugccgau gaacucaggg cucgccugag uaagcacccu     2340

aaagagucca uuaaggcccu aaaccuggga aauuacgaca acgagaagcu cgaaagccug     2400

cacccagugu ucgucucucg aaugccgaaa agaucaguga caggcgcagc ucaucaggag     2460

acccuucgga gguauguugg uaucgacgaa cgcaguggga agauucagac ugugguaaaa     2520

acaaaguuga gcgagaucaa acuggaugcc uccggccacu uucccaugua cggaaaggaa     2580

ucugacccuc guacguauga ggcgauaaga caacgguuac ucgagcauaa uaacgaucca     2640

aagaaagccu uccaggaacc ccuguacaag ccuaaaaaga auggcgagcc agggcccauu     2700

aucaggaccg ucaaaauuau cgacacuaag aaccaggugg uuccgcuuaa cgauggaaaa     2760

acaguggcuu auaauucgaa cauaguccgc guggacguau uugaaaagga ugguaaguac     2820

uacugugugc cuguuuauac cauggacauc augaaaggcg uccugccaaa uaaggcaauu     2880

gagcccaaca aaccuuacag cgaauggaag gagaugacag aggauuauac uuuccaguuu     2940

uccuuguacc ccaaugaccu cauccgaauu gaacugccaa gagagaaagc cauccggacc     3000

ucaacggggg aagagauaaa gauuaaggau uucuuugcuu auuacaaaac cauccacagu     3060

ggaacaggcg guauggagcu aaucucucau gacaggagcu ucucccuguc aggggugggc     3120

agccgcacuc ucaagagauu cgaaaaauau caaguggaug uccugggaaa cauuuacaag     3180

guuaggggcg agaaacgggu ggggcuugcc ucuuccgcac acaguaagac cggaaagaca     3240

gugcguccuu ugcagagcac ucgcgacuga                                      3270


<210>  2739
<211>  3273
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2739
augagaugga ggcugggccu cgacaugggg gccggaagcc uugguuggug cgcugugggc       60

cuggaugacc agggguaccc cgauuccauc uuggcagccg gaucucacau uuucggcgac      120

ggucgggcgc cuucacgcgg ggaucaacag ggaaccccau uaggcgucga gcgacgugcu      180

gccaaggcaa ugagaaggcg gcgcgacaga uuucugggca ggcaggccaa gcuacucgcu      240

cggcugacac uugcagggcu guucccggcc gaucccguuc gcgcuaaggc cuuggaacuc      300

cuggacccuu augugcugcg agcgagagca cucgaugagg ccuuaccacc ccaucagcug      360

ggaagggcuu ucuuccaccu uaaccaacgg cgugguuuua aaaguaaucg caagacugac      420

agaggcguag augacgaagc cgggaaaaua gcaaucggaa uugauagguu gcgggcugcc      480

aucgcggccg cagaggcucg aacgaugggc gaguuccugc auaagcgcag acagagcgcc      540

ccugacguga accagauucc auccgucagg acccggcuac gccccgagac aggugaggaa      600

gcuaaggggg acggauacga uuuuuauccu ggcagagagc ucguggaaga ggaguucgac      660

gccaucuggg augcacaggc cccacaccau ccgucucugc uuacucccga caauagggcu      720

cuguugcacg aagcgauauu uuaccaacgu ccucucaaag caccaaagau cggcaaaugu      780

acccuguuaa acaaugagcc ccggcugccu aaggcccacc cacucuucca gcgaagaagg      840

cuucuggaag agguuaacgc uuugcgcauu gugcggcccg gggaagucgc cacaaaacua      900

accugcgagc agagagaucu gcucaugcug aagcugaagg acaaagcaaa ggugucguuu      960

gagacucuga ggucaaaagu auugaagcuc gaaccggagg cccgcuucaa uaaagaagga     1020

gagaaccgaa cagaacuggu uggugaugag gugugugcuc agaugagcgc caagacccgg     1080

uuuggcaaag caugggcgca uuucacggac gagaagcguu gggcuaucuu aaguagacug     1140

auugaagagg aagaugagga aaggcuagac acuuggcucc gcaccgagca cgggcuggau     1200

gccgagcaag cacgggccgu cgcuggagcc agacuuccug caggcuucgg uagguuuggg     1260

gaaacagcua cucgacgcuu gaucgccgcg cugcaggccg aggugauagu guauuccgaa     1320

gcugucguug cagccggccu cggacaucac ucugacaugc ggaccggcga ggugcuggcu     1380

gagcuucccu acuaugggau gacacuggaa agacacuugc ucccaggaac cggugacaag     1440

gccgauccug aggaacugag gauuggccgu uuaacgaauc ccacuguaca uaucgcacug     1500

aaccgccuac ggagaauugu gaauaggcuc aucaaaacau acgggcgacc agagcagauc     1560

gcgauugaac ugggacggga gcuuaaguug accgacgagg auaaacaacg ccugagcaga     1620

cagaacaaua ggaaccgggc cgacgcugaa gcccgaucca agaaacucgc agagcugggc     1680

caggcugaua auggugccaa cagagcacgc uuaaagcugu gggaagaacu uaacagggaa     1740

aaugccuugg accgucggug cauauacuca gggcagcaaa ucaguauugc uaugcucuuu     1800

agcgaugcgg ucgaggugga ccacauccug cccuuugaug ccacucuaga cgauucuaac     1860

aauaacagaa uacugugucu cagggagugg aaucgccgaa agagaaaaca guccccuuau     1920

gaagcauggg gccauacaga cuacuggcca gauauugcug cccgggccgc uaggcugccg     1980

aaggagaaac gcuggcguuu cgaacccgac gcaaugcaga gauuuucagc cgaggaccgg     2040

uucaucgcga ggcaccuugu ugauacccag uauaugagcc gcuuggcucg agaauaccug     2100

gaggccuuau auccugagaa gggagaaggc agugggcaug ugugggucuc uccaggaaga     2160

cucacggagc ugauccggag gaaacuuggu cugaacgaau ugcuccccga ccacaauuuu     2220

gcaggcguga agaaccgccu ggaucacaga caucacacca uugacgccuu cguaguugcu     2280

gugacagaua ggucccuacu gcaagggauc agccgggcau cggcccucga gggagaggaa     2340

ggccgugagc gcgucauugu gccugaacca ugggacgguu uucgagaggc gcugagaacu     2400

acccuuaaua caauaacugu cuggcauagg caggaucacg ggaccgcuuc uaaggccggc     2460

uuggcacagg gacgggacca gacagccggc aaacugcaca acgauaccgc uuacggguua     2520

acuggacgcc aagacacgaa gggcaaugau cucgugguuu ccagaaaacc ccugauguca     2580

cuuaagccgg ccgacaucga accugugcca gauguagcac ccauuacaaa aggugucagg     2640

gaccgggacc ugcgaaaggc ucuagcccau uucacccagg cgacugaggg gaagggauuu     2700

cuccaggccc uggagagauu cacccgcuug ggcccucugc acuuuagggg uauccggcgu     2760

gugagauuaa uccagccccu cgcaguuauu ccaauaaaag gcccuggggg agugcccuau     2820

aagggcuacc uugggggaga aaacuucagg ugcgaugucu gggagaugcc agacggcgcu     2880

uggaaaccgg aagugacaag uauguuccaa gcccaucagc cugguuggac cagcgcuauc     2940

cgcgcagccu gucccacugc ccggaaggua cuuucccugc acaaacgaga uaugauugcu     3000

aucgagagag aagagggggu gaggacauug augguugucg cgcagauuug gucuaaugga     3060

caagugacgc ucguggagcc acaugaagca ggcgaccuga acaagcgcaa uaaaagcgag     3120

gaugaccccu uugguuacuu caugccuacc gccggcgggc uaaagcggcu gaaggcuaga     3180

cagguccgug uugaugaacu uggaagggug caggacccag gcuuucccgc ccggaaagca     3240

cgccgaagaa cuaggccuaa acagguggau uga                                  3273


<210>  2740
<211>  3273
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2740
auggacugga gacugggccu cgaucuuggg gccaacagcc ugggaugggc ugcauugagg       60

uuagaccggg ccggugaucc caucggcauu cuggcgaugg ggucccgcau auucucugac      120

ggacgagauc cuaagucagg cgcuagucua gccguggacc guagagcagc cagggcuaug      180

cggcgcagaa gggaucgguu uaaacagcgc caagcagccc uccugaagua ccuuauccga      240

gacggucugu ucccagcuga ucaggccgac agacagaggu uggcggauga ggacccguau      300

gcacuccggg cccgugcucu ggaugaagaa cugagccucu uugaaauugg gcgcgccuua      360

uuccaccuga aucagagaag gggauuucaa uccaaccgga aagcagaccg aggccaggac      420

gaugaggcug gcaagaucgc cgucgggguu gaccgccuua aucagcagau ggaggagcaa      480

ggagcgagaa ccuuggguga guuccugcau cagaggcggc gcgccgcaac agaugcuaac      540

cacauucccu cugugagaac uaggcuacgu ccugaaacgg gcgagggggc caaaggagac      600

ggcuacgauu uuuauccauc gcgggcucuc aucgccgaag aguucgagga aauaugggca      660

gcccaggcuc cccauuaccc ugacacccug acacagcaag uacacgaucg acuuuaugag      720

aucauuuuuc aucagagacc acugagggcg ccccaggugg gucaaugcac uuuggucgca      780

ggggaaaccc gccucccuaa ggcccaccca cuguuccaga agcggagauu acuggaggaa      840

aucaaugcuc ucaugauugu gaggccggga gagaucgccc agcgccuuac acccgaggaa      900

cgagacaccc uguacuugaa acuaaaggau aaacggacug uuucauuugc aacacugcgu      960

aagacccuca aacuggacag cgccgcuaga uucaacaagg agagugaaaa uaggacggag     1020

cuuaagggcg augaaguggc cgcagagcug ggcgcgaaaa cucgcuuggg gacccggugg     1080

cagcaccucu ccggagacca acagugggag gucgugcaua gacugauggc uuuagaaucu     1140

caggccgacg aggcagccuu uagggauugg auaagccaga cauaugaccu gcuaccugaa     1200

caagcugccg caguacucgg ugcucgacug ccacagggcc acgggcgcuu cggacccacu     1260

gccaccacaa cccuuauuga uuccuugcug caugaccgga cggaggaugg cagaguugug     1320

gucuacucag aagcggugca gaggaguggu cucgggcacc acagcgaccu gcgucaugau     1380

cgcgacgaug aggacgugac ugagcuuccu uacuauggcg ucgcccugga acggcacauc     1440

augccaggaa cagcugagcc caccgauccg gaagagauga gaauuggcag guugacuaac     1500

ccuacaguuc auaucgggcu caaucagcug cgacacgugg uaaaccgguu aauccgcgca     1560

uacggacccc caaaggagau ugccauagaa cuggcuagag agcuaaaacu cucugaagac     1620

gagaagaaaa agaggaacuc cgaaaaucgg gauaaucgac uggccgcaga ggguagauca     1680

aagaaacuug aggaaauccg ccaaagggac accggcgcga accgugccuu gcugaagcuc     1740

ugggaggaac ugaauccugg gaacguguua gaccggagau guauuuauac gggagagcag     1800

aucagcauag auaugcuguu cagugacagg gucgaagugg aucauauucu ucccuuugcu     1860

cagaccuugg acgauucuaa cggcaauaaa cuccugugcc uacgcgaggc caaccgagag     1920

aagagacgga gguccccaua cgaagcaugg ggucacacag accgcuggcc cgauaucgcu     1980

gagcgugcca gcagacugcc uaaaccaaag cgguggaggu ucgaaccgga cgcaaugcag     2040

cgcuuugcca cugaggaugg guuccucgcu cgacaccugg uugacaccca auaucuuggc     2100

agauuggcgc gggaauaccu gucgugguua uaucccggaa ggggcgaggg gucuggacau     2160

gugugggucu ccccuggucg ccucacagag auggugagaa ggaaacuggg ccuuaaugau     2220

cuguugccag accacaacuu ugggggaggc gccgaucagc ccaagaaucg gcucgaccau     2280

cgucaccacg caaucgacgc ucugguaguu gccauugugg aucgcgguau gcuacaggcc     2340

aucgcucgag caucagggca agacggcagu gaaggaagag agaggauuca uauaccugcc     2400

ccaugggaug gcuuccggga cgaucugcgc gucgcgguga acgggaucau ugucagccac     2460

agacccgacc auggaacugu ggcuaaggcc ggccucgcaa ggggucggga ucagaccgcc     2520

gggcgacugc acaaugacac agcuuacgga cuuaccggcg aaacugaugc aaaagguaac     2580

uccaucguug ugagacgcau cccguugucu gccauuaaga aaccugcgga cauagcuagg     2640

guacgggauc cacuguuacg uaaugcccuc gcagaguuua cgaagggccu ggacgggaaa     2700

gccuucgagg cuagaaucgg aagguuucau gaacuuggcc cccugcuaua ccgcgggauu     2760

cggcgaguca gagugguuga gacacucacc gugaucccua uuagggacgc cgaaggacgc     2820

gcauauaagg gcuacaaggg ugauagcaac uaucgguacg acgucuggga gaugcccgau     2880

gggaaaugga gaugucaggu gguaucaaug uucgacgcuc accagaguac ugaacaagcc     2940

cguccacacc cugcggccag gaagauccug uccuugcagc ggaaugauau acuggcaauc     3000

gagcgcaccg agggagaccg agaauuagug agaguuguca aauuuagcga gaagcaguuc     3060

gcuguggcuc ccccacauga aggcgguucu cucaaaacaa gggauggcaa cgccgacgau     3120

ccguuucggu aucuguaccc uucacccaau acccuuaagg gguggcgcgc aagacaggug     3180

aggauugacg agcugggacg agucuuggau ccaggcuucc ccgcccggaa gaguguucgu     3240

agaacucgcc cuagggccgc uccaguggac uga                                  3273


<210>  2741
<211>  3276
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2741
auguacagca accuccuguu cuccuuugac aucggcacca auucuauugg guggugcgug       60

uucgcccuug augaggcugg agaacccuau agaauagucg accugggugc aaggaucuac      120

gccgauggcc gggacccuca gucaaagaca aguuuggcgg uugcucgccg agaggcccgu      180

gcaaugagca gaaggcggga ucgcuauuua agaaggcgga aagccacucu gcgcacgaug      240

auugaauacg ggcuaaugcc aggagacaag gcuggccaag aggcagugcu ccgagaaacc      300

ggugauagag aagggggaga cgaguccuuu aacccguaug cccugagggc ucgggcccuu      360

ucugaaaaac ugcccccugc guacaucggc cguguauugu uccaccucgg ccagcgcaga      420

ggguuuaagu cgaauaggaa aacagaucgg aaggacaacg auaagggaaa aauugcacug      480

gguaucgacg agcugacugc cgcuaugcau cgaucaggca gcccaacccu cggggccugg      540

uuagcaaugc gcagagcuga aggacacagu gugaggcugc gggccggcuc cgaggucuuc      600

gaugcggagg guuaugccuu uuaccccgaa cgcucucuuu uggaggacga auuuagacag      660

auaugggacg cacaggcugu gcaucacccu gauauccuga caccagagag gcgugcccau      720

cuauuccggg uuauguucua ccaacgaccc cucaagaaac cucugguggg gagauguagc      780

uuuaauccag cugaguucag gcuuucccgc gcccacccgc uguuucagga guuccgguug      840

uauaaggaga uuaacgaccu cgaagucgug cugcccgauc agucacacag aaaauuaacc      900

cuggacgaga ggaaugcacu cguagccaaa cuuaagacuu cccgcaaagc uagcuucucu      960

gcgcugcgaa agacauugaa acuaaccccu gaucugguuu uuaacaagga auccgaggca     1020

cggaaagaac uccugggaga cgagaucuac ucagugcuug ccgaugcuaa gauguucggc     1080

gcccguuggg gcggguuuag cagagcaagg cagugggaga uuaucacgcg ccugaaagaa     1140

gaggaagacc cagccaguuu gucuuccugg cucaagagcg aguucggacu ggaugacgaa     1200

cgggucacug cuauagccaa ugcaucguua cccgaugguu auggcagacu gggggagacc     1260

gcgcuagcuu cuaugcucga ggaacugaag acagccguga uuccugaguc cgaagcagcc     1320

aggcuuugcg gauacgacca uucaaacuug gcuaaagagc aagacgaggg ccuggauacu     1380

cucccagaau aucaggagau ccuggaacga cacauucccc cggguaccgg ggacccugau     1440

gacaucuacg aucuucgcaa gggccggauc acaaauccca ccgugcauau uggacugaac     1500

caguugagaa gggucguuaa ugcccucaua caccguuuug gccgcccaca gcggaucgug     1560

guagagcugg caagagacuu acaacugagu gaaaaacaga aggaugacgu gaacaggcga     1620

auugcuaaaa auacgcggga ggccgagggg cgcagccaga agcuaaucga aaugggacaa     1680

cucgauacug guuauaacag acugcuuuug aagcuguggg aggaacucaa ccuggacaaa     1740

ccugaggaua gggucuguau auacuccggc aagcccauug ggaucgcgau guuauucucu     1800

ggagaagugg acguugauca cauccugcca uggagcaaaa cacuugacga cucacaggcc     1860

aauaaguugc ucugccugaa aagugcuaac cggcagaagc gaaauagaac ccccuccgau     1920

gugccugagu ggcgcgacag guacgaugag guccuagcac gugccgcucg gcugccaaga     1980

aacaagaggu ggcgcuuugc cgcagacgcg augcagcaau ucgaagccga gggcgguuuu     2040

cucgcucgac agcugacuga uacacaguau cuuagcagaa uggccuugga auaccugucu     2100

gcauuauauc cgucagagga agcugacaaa aguggggagc uccggcagag gaagcgcgug     2160

cauguaguuc ccggccgucu gaccgagcuu cugagacgga auuggggauu gaacacgcuc     2220

cugccugauc acaaucuagg cgaaauggcc caagagaaaa acaggcgcga ccaucgacac     2280

caugcaauug augccgcugu gaucgggacc acaagcagau cccugcucca gcggaugucu     2340

ggagcggccg caaggcugga cgaugucgcu uucgacgauc uugugcgcac ugucgugaag     2400

gacaauccac ccuggccugg uuuuagagaa gaguugcugg ccgccauugg caggguuacc     2460

gugucgcaca aaccagacca cgggacagua agccgggcug cauacgccca gggaaagggc     2520

cagacugcgg guaaguuaca uaacgauacc gcuuaugggc ucacaggccu ggucgacgcc     2580

aaaggaaguc cccuuguggu ucgucgcaag ccguucaugg cacuggaggu gaaagauaua     2640

gccucaaucc gagacaccga acuacaaagu gcucucuacg gcgcaauugg gucucugacu     2700

gagaagaaag ccuugcagga agcgcugauc agauuuaggg auacgcaccc ucaguucaag     2760

ggaauccggc gcgucagagu guuagagaca cucagcguaa uuccaauagu ggacagggau     2820

ggcaaggcuu auaaggguua caagggggac gccaauuacc gguaugaagu uugggagacc     2880

cuggauggaa aauggcauac ugaggucgug uccauguucg acgcacacca gcccggcugg     2940

caaucaccuu uucacaagca gaaccccgcc gcucgaagag ugcuuaaacu gcagcaaaau     3000

gauauggucg ccuacgaaca uccagcagac gguuauacca ucgcucgcgu ugugaaguuc     3060

agcaacgaca aaaggauuua cuuugccucu caccgggagu ccggcaguuu gaaggcgcgu     3120

gaugccgaca agggggaucc uuucagcuau uuugcaaaag cuaauaacgg acucagagac     3180

aucaaguguc gccaggugag gauugaugcc gcuggccgga ucuucgaccc cgggccacag     3240

gaucgagaaa caagacaggc aaacaaagga guauga                               3276


<210>  2742
<211>  3279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2742
auggccuaca ccaugggcau cgacgugggg auugcuagcu gcggaugggc aauagucgau       60

cuggagagac agaggaucau ugacaucggu guucggacau ucgaaaaggc cgagaacccc      120

aaaaauggcg aagcgcucgc ugugccucgc cgagaggccc guuccucuag aaggcggcuu      180

cgcagaaaga aacacaggau ugaacggcug aaguauaugu uuguacgcaa cggguuggca      240

guggauaucc aacauuuaga gcagacucug cgaucacaga augagauaga cgucuggcag      300

cuaagagugg auggacucga caggaugcug acgcaaaaag aauggcuucg gguucugauc      360

cacuuggccc agcgucgcgg cuuccagagu aacagaaaga ccgaugguag cuccgaggac      420

ggacaggugc ucgucaaugu gacagaaaac gauaggcuga uggaggagaa ggacuaccgg      480

acuguagcug aaaugauggu uaaagaugag aaguuuucug accauaaacg aaauaagaac      540

gggaauuauc acggcguggu cucgcgcgca ucacugcucg uggaaauuca uaccuuauuc      600

gagagccaaa gacagcacca caacuuucug gccaguaaag auuucgaacu ugaguacauc      660

aauauuuggu ccucucagag gccaguggcu acaaaggacc agaucgagaa gaugauaggc      720

accuguacau uuuugccgaa agaaaagcgg gcccccaaag cgagcuggca uuuccaauau      780

uuuaugcugc uacagacuau uaaccacauc cgcauuacca augcacaggg gacgagaccu      840

cucaacaagg aggaaaucga gcaagucguu aauauggccc ugacuaaauc caagguguca      900

uaccaugaca ucaggaaagu acuugaucug agugaagagu aucaguucgu gggauuggac      960

uacgguaagg aggaugaaaa gaaaaagguc gagagcaaag aaaccauuau aaagcucgac     1020

gauuaccaca aacugaacaa gaucuuuaac gagguggaau uagcuaaggg cgagacaugg     1080

gaggccguug acuaugauac uguggcauac gcucugaccu ucuuuaaaga cgaugaagac     1140

auucgugauu aucuccagaa uaaguacaaa gacucuaaga accggcuugu caaaaaugug     1200

gccaacaagg aguauacaaa ugaacugauc gagaagguau ccaccuuguc auuccgaaaa     1260

guugggcacc uaagccugaa ggcgcucaga aaaauaauuc cauuccugga gcagggaaug     1320

acguacgaca aggccugcca agcagcuggc uuuaacuucc aggguaucag uaaaaagaag     1380

aggucugugg uccuucccgu gaucgaucag auuuccaauc cugucgugaa ccgcgcccug     1440

acucagacac ggaaaguuau caaugcuuug auuaagaaau augggagccc agaaaccaua     1500

cauaucgaga cugccagaga acucucgaag agguuugacg agcgcaaaaa cauuacaaag     1560

gauuacaaag aaaaucgaga caagaacgag cgggcaaaga aacaccuguc ugaguuagga     1620

aucaucaauc ccaccggccu ggauauugug aaguacaaac uaugguguga acaacagggc     1680

cguugcaugu auuccaacca gccuauauca uucgagagac ucagggaaag uggguacacg     1740

gagguagacc auaucauucc auauagccgc uccaugaaug auucuuacaa caaccggguc     1800

cuggugauga ccagagaaaa uagggagaaa agcaaccaga caccguauga guacauggga     1860

aaugacacuc aacgauggga ugaauuugag cagcgcguua ccacaaaccc ccagaucaaa     1920

aaggaaaagc ggcagaaucu uuugcugaaa gguuucacua acagaaggga gcucgaaaug     1980

cuggagcgua aucuuaacga cacccgccac auuacaaagu aucugucaca uuuuaucagu     2040

accaauuugg aguucucccc uagcgauaaa aagaaaaagg uggucaacac uucuggccgg     2100

auaacgucac accucagaag uagguggggg cuggaaaaga aucgagagca aaacgacuua     2160

caccaugcca uggaugcugu gguaaucgcg gugacaagcg acuccuuuau ucagcagguu     2220

accaauuacu acaaacggaa ggaacgcaga gagcugaacg gagacgauaa auucccacua     2280

cccuggaagu uuuucaggga ggaagucauc gcacggcucu cuccuaaucc aaaagagcaa     2340

auugaagccc ugcccaacca cuuuuauucg gaggacgaac uugcugauuu gcagccgauc     2400

uucgugagcc gaaugccuaa gagauccaua acuggcgagg cccaucaggc acaguuccgc     2460

aggguggucg guaagaccaa agaggggaag aacauuacag ccaaaaagac cgcucugguu     2520

gacaucucau acgauaaaaa uggcgacuuu aacauguaug gacgugaaac ugaucccgcc     2580

acauacgagg caauuaagga acgguaucuc gaguucggcg ggaaugcgaa aaaggcuuuu     2640

aguacggacc ugcacaagcc uaaaaaggau ggaaccaaag guccauuaau caagucugug     2700

agaauaaugg aaaacaaaac ucuggugcac cccguaaaua agggcaaggg ggucguguac     2760

aacagcucca ucguuaggac agacguguuc caacgcaaag agaaguauua ccuuuugcca     2820

gucuacguga ccgauguuac aaaaggaaag cuccccaaua aagugauugc cgcaaagaag     2880

ggcuaugacg auuggaucga gguagacgac ucauuuacuu uccuguacag ccuauauccu     2940

aacgaucuga uuuuuauccg acagaaucca aagaagaaaa uaucucucaa gaaaagaauu     3000

gaaucccaua guaucagcga cucuaaggag guccaggaaa uccugaccua cuauaagggu     3060

guggauuccu caacggccgc uauugaguuc aucacccacg acgggaguua cuaugccaaa     3120

ggcgugggag uccagaaccu ugauuguuuu gaaaaguacc aaguugacau uuugggcaau     3180

uacagcaaag ugaaggggga gaaacggcug gaguuagaaa caucggauaa caaucauaag     3240

ggaaaggacg uaaacuccau aaaaucuacu agcagguga                            3279


<210>  2743
<211>  3279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2743
augaaguaca gcaucggccu ggacauuggg auagccuccg ugggaugguc ugucaucaac       60

aaagauaagg agagaauuga agacaugggu guuaggaucu uccagaaagc ugagaauccc      120

aaggauggcu caagucucgc aagcucccgg cgcgaaaaac gagggucucg uagaaggaac      180

cggcgcaaga agcacagacu ugacaggauu aaaaauaucc ugugcgaguc gggauuggug      240

aagaaaaacg aaauagagaa gaucuauaaa aaugccuacu uaaagucacc uugggagcug      300

cgggcuaaga gccuagaagc gaaaauuagu aacaaggaga ucgcccaaau ucuccugcau      360

aucgcaaaac gccgaggcuu uaaguccuuc agaaaaaccg auaggaaugc cgacgauaca      420

gguaaguugc ugucugggau acaggaaaac aaaaagauua uggaggagaa gggauauuug      480

acuaucggcg acaugguagc uaaagaucca aaguuuaaua cgcacgugcg gaacaaagca      540

ggcagcuacc ucuucuccuu uucacguaag cugcuggaag acgagguccg caaaauucag      600

gccaagcaga aggaacucgg gaauacccau uucacagaug acguguuaga gaaauauauc      660

gaaguuuuua acagucaaag aaauuucgau gagggaccga gcaagcccuc uccuuacuau      720

uccgagaucg gucagauugc uaaaaugaua ggcaacugua cuuuugaauc aagcgagaag      780

aggaccgcca aaaauacaug gaguggggaa cgguucgugu uucugcagaa acuuaacaac      840

uuccgaaucg ucggauuguc uggcaagcgc ccacugaccg aggaagagag agacauugug      900

gagaaggaag uauaccuaaa gaaagagguu agguacgaaa agcuccggaa aauccuguau      960

cuuaaggagg aagagagguu uggugaccug aauuacucca aggaugagaa acaggacaag     1020

aaaacugaaa agacaaaauu cauaagccuu auugggaacu auaccaucaa gaaacucaau     1080

cugucggaga aguuaaaguc ugaaaucgag gaggauaaau ccaagcugga caaaauuauc     1140

gaaauucuca cguucaacaa gucagauaaa acuauagaga guaaucuuaa gaagcuggaa     1200

uugagcagag aggacaucga aauucuacug uccgaggagu uuucuggaac ccucaaccug     1260

agccuuaaag cgaucaagaa aauccugccc uacuuggaaa agggccucuc auauaaugag     1320

gcaugcgaaa aagccgauua cgacuauaag aacaauggca uuaaguucaa acgcggggag     1380

cuguuaccug uggucgauaa ggaccugaua gcuaacccag uggugcuacg ugccaucagu     1440

caaacacgga aagucguuaa ugcaauuauc cgaaaguacg gaacucccca caccauucau     1500

guggaaguag cuagagaucu cgccaaaucc uacgacgaca ggcagacaau cauaaaggag     1560

aacaagaaac gcgagcugga aaaugagaag accaaaaagu uuaucagcga agaguucggu     1620

auuaaaaacg ugaagggcaa acuuuugcug aaguaucggc ucuaccagga acaggagggg     1680

agaugugcgu auucuaggaa ggagcuguca cuuagugaag ucauccugga ugagagcaug     1740

acggacauug aucacaucau accuuacucc cgcucuaugg acgauucgua uagcaauaaa     1800

guguugguuc ucuccggaga aaaccgaaag aaaucaaacc uguuaccaaa ggaguacuuu     1860

gaccggcaag gccgugauug ggacacuuuc gugcugaaug ucaaagccau gaagauucac     1920

ccgagaaaga aaaguaaccu acucaaggag aaauuuacaa gggaagauaa uaaggacugg     1980

aaaucucgcg cacugaacga uacccgguau aucagcagau ucguggcuaa uuaccuugag     2040

aacgccuugg aauacaggga cgauuccccc aagaagcgag uauuuaugau uccuggucag     2100

cugacugcuc agcuccgcgc ccgguggaga cugaauaaag uuagggagaa cggggacuua     2160

caucacgcac uggacgccgc uguggucgcg gugacagauc aaaaggcaau caauaacaua     2220

ucaaauauca gccguuauaa agaacuuaag aacugcaaag acgucauucc aucuaucgag     2280

uaccaugccg augaggaaac cggcgaggug uauuucgaag agguuaagga cacgcgcuuu     2340

cccaugccuu gguccggauu cgauuuggaa cuccagaagc ggcuggagag ugagaaucca     2400

agagaagagu uuuacaaccu acugagcgac aaaagguauc ucggcugguu caauuacgaa     2460

gagggguuca uugaaaagcu gcgacccgug uuuguaucuc ggaugccgaa ccgcggaguc     2520

aaaggucagg cucaccagga gaccaucaga uccucaaaga aaauaaguaa ccaaauugcc     2580

gugagcaaga agccucuuaa uucgaucaaa uugaaggauc uggagaaaau gcagggcagg     2640

gacacagauc ggaaguuaua ugaagcacuc aaaaaccgac uggaggaaua cgacgauaag     2700

cccgagaaag ccuucgcuga gccauuuuac aagccuacua auuccgggaa gagaggaccc     2760

cuuguucgcg gcaucaaagu ggaagagaag cagaacgucg guguguaugu aaaugggggc     2820

caggccucua acggaagcau ggugaggauu gacguuuucc guaaaaaugg caaguuuuac     2880

accgucccaa ucuaugugca ccaaacacug uugaaagaac uccccaaccg ggcaauuaau     2940

gggaagccuu acaaggauug ggaccugaua gacggaucau ucgaguuucu auauaguuuc     3000

uacccaaacg aucugaucga aauugaguuu gguaaaucca agucuaucaa aaaugacaac     3060

aagcucacua aaaccgagau uccggaagug aaucugagcg agguccuugg cuauuacaga     3120

gggauggaua cauccaccgg agcggcuacu aucgacacgc aggauggcaa gauacagaug     3180

aggaucggua uuaagacagu uaaaaacauc aagaaauacc agguggacgu guuggggaau     3240

guauauaagg ucaaacgcga aaagcgacaa accuucuga                            3279


<210>  2744
<211>  3285
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2744
augaaaagca cccuggccau cgcuuccauu ucuucacucg gcugggugau agagcuuggg       60

gaaaacagaa ucaugaggcu gggauuggac auugguagua caagcaucgg cugguggauu      120

uacgagacua cggaugggca gaucaccucc gucauagacg gaggcguucg gaucuucucu      180

gauggucgcg accccaaguc gaagggguca uuagcagugg aucgacgugu agccagagcg      240

caaaggcggc gcagagacag guaucugcgg cgcaaagcug cccuaaugaa gcgaauggca      300

gaagugggac ucaugccuag cgauccaaaa caggccaagc agcuggaggc ucuugacccg      360

uacgaacuga gagcaagggg cuuggaugag acacucgagc ugacugaacu gggccgggcc      420

cucuuucacu uaaaucagcg ucgcggguuc aagaguaaca gaaaaaccga cgcuggagau      480

aaugaguccg guaagauuau cgacgccaca gcgaggcugg auugggcaau gcgggaccau      540

cgagcccgca ccuauggcga auuucuucac augagaaggc aaaaagcuga cacuccccgg      600

acaaccccug ccguccgcac gagauugacu auugcagcua gggauggggc cgagaaggag      660

gaaucuggau acgacuucua uccagaucgu cagcaucugc gggacgaguu ugaugcgcua      720

ugggaagccc aggcagagua ccacggcgug cucaccgaag accugcgaga ugagaucggu      780

acaauaauuu uccaucagag accccuuaaa acuccuaagg uugggaagug ccuguuuacc      840

aacgaggaaa ggguggcuag cgcccaccca uugaaucaac gccggauccu cgucgagaca      900

gugaacgcuc ugagaguagc ccaguuagga gaaguugcaa gggcccugac ccucgaggaa      960

cgcgacacga uucuucacgc ucuggauaau aaagcgcaua cuaaguccuu gucaggcaug     1020

aguaucaaac uaaaggcacu gggcaaagug aucaagcucc gaagcgggca guauuucaca     1080

cuggagaccg ccaaccggga cgcuauugcc ugugaucccg uccgugcauc ucuuuccaaa     1140

ccugacagaa uaggaucaua cuggagcacu cuggaccaca augcccagug ggagguggug     1200

aacaggaucc gcgcugucca aagugaugcc gaccaucggg aauugguugc auggcucgug     1260

ucucagcaca gacuggcggc ugagcacgcc gaaggugcag ccuacgcucc auuaccggau     1320

ggcuauucca ggcuggggau uacagccacc aagcgaaucc uagcagcucu cgaggccaau     1380

guaauacccu acagcaaggc gguggaaucg ugcggauggc aucacucuga cgagcgcacg     1440

ggcgaggucc uggaucggcu uccuuauuac ggugaaauuu uggacagaca ugugauccca     1500

gggaccuaug augacuccga ugacgauauc acaagguuug gccguauuac uaaccccacc     1560

guucacaucg gacugaauca gcuccgccgg cuggugaaca gaauuauaga gguccauggc     1620

aaaccugacg aaaucguggu agagcuugcc agggaucuga aguugucaca agaccagaaa     1680

gcugacguug agcgaacaau uaagaaaaau acucaggcag ccauccggcg cggggaaaag     1740

cucgaggaaa ugggagugcc aaacaccggu ggcaacagaa ugguccugag guuaugggag     1800

gaucugaaug acgaugacgu gaugaagcgg uucugucccu acacagggca gcgaaucagu     1860

gcuggaaugc uauuugaugg cagcugcgac gucgaucaca uucucccguu cuccagaacc     1920

cuggacgaug guuuuacuaa caaaacgcuu uguuugaagg aagccaaucg caaaaagagg     1980

aacuaugcac cucacgaggc ggccgagaaa gggcugcucg accgugaugc uauggacucu     2040

guggccaagu accugcccga uaauaaggca uggcgguucg cuccagacgc cauagaaaga     2100

uucgagggcg aaagggacuu uacagcacgc gccuuaaaag auacccaaua ccugagccga     2160

cuugcuagaa cuuauuugga cgcgcucuac gauggaggcg acgauaaguc acauguuugg     2220

gugguaccug ggcggcugac cgagaugcua aggcgccacu ggggacugaa cggucucgaa     2280

gcccuggcag acagugauga gaaaacaguc aagcguaaaa auagaaccga ccaucggcac     2340

cacgcuaucg augccgccgu gguugcugca acugacaggu cccuuauuca gaagaucagc     2400

aagauugccg cgucugauga gcaggcuggc gccgaagaca cauugaaauc aacgccccca     2460

cccugggaca guuuccgcga ugacaucgca gcccagcugc aaaacauaac cgugagccau     2520

cgagcugauc acgggagaac ugacuccuua gcaaagggac ggggcgagga uacaaccucu     2580

ggugcccucc augaagcgac agcucugggg aucauugacg agacucacgu caccgugagg     2640

aucccuauug aaucgcuuaa aagcggccau cugaucgagg gaucccgcaa uggcuauaua     2700

agagauccau acuugaggga ccagcucacg cuggccacca agaacggguc agauaaagac     2760

uuuacaacug uacuacagaa guucuuugau cggaguaaac guuauggagg cacccgccga     2820

gugagaauua ucaagacacu gcaggacacu gcaaggauuu cuguuccgca caaugccccc     2880

aucaaggcuu acaaaggugg gagcaaccac gccuucgaga uauggcggcu cccuaaugga     2940

aagaucgaau cccaagucau uaccacgugg gagcaucaca accugacaga accaaaacgc     3000

ccccauccug cagcuaagag aaucauuagg cuucacaaaa ccgauauggu ggccaucgag     3060

cgggacggca agacugugau augcuuuguc cagaagacag acccaucaaa uggugcguuc     3120

uugguucccc auuuugaggc caacgcagau ggccgaaaaa gaaagaacga accgguggag     3180

ugguaucaga ugagcgcuaa uucucugauu aaagccgacu uacgcagggu ggggguagau     3240

gaaaugggac ggguccguga cccuggccca cccccuauca gauga                     3285


<210>  2745
<211>  3285
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2745
augaaguaca gagugggcuu cgaccugggg accaacagcc ucggaugggc cgucguuagg       60

cuuaaugcuu ccggugagcc cgaucggauc gaaguggcag gcucucgcau auuuucagac      120

gggcgagagc cuaaaagugg agccagccug gcgggcgcuc guagagaugc cagggcaaug      180

cggcgcagaa gggaccgguu caagcagcgc caagccgcuu ugauuaaaua uuuaacacag      240

gcaggauuuu ucccagccga ugaauccgag cgaaaggcuc ugucugcccu agacccguac      300

gaacucagag cgagggcacu ggaugaggcc cuuccccugg cucacuuggg gcgggcccuc      360

uuucaucuga accagcgucg cggauucaaa ucgaauagaa agacugacag gggcaagaac      420

gaugacacgg gcaaaauccg gauuggggua gcacgacugu augagcagau gcgcgaugcu      480

gacgccagaa cccucggaga auuuuuacac aagaggcggg cggccgcauc agauccuaau      540

gcuaucccac augugcgcac aagaauuguc acucuggccg cugaggacgg uaaagccagc      600

gacguguacg auuucuaucc cgacaggcaa acccuugaag aggaguuuga agcaaucugg      660

gaggcccaga caagucacca uccugaggcu uugaccgagg aacgucgggc gcaguucuac      720

gagauaaucu uucaccagcg accacugaag caacccaaaa uuggcuucug cacucuacuc      780

ccuccagaga agagacugcc gaaggcacac ccccuuuuuc agaggcgccg gcuguuggaa      840

gagguuaacg cccucagaau cgugcugccu gggaaagaaa caagggcuuu agagaaggcc      900

gaacgcgaug cacugcucuc caaacuuaau cagcgaaaga aagccgcuua ugagucucug      960

cggaaggccu ugaaacuaga cccagaugca cguuucaaca aggagagcga caauagagaa     1020

gauaugauug gagacgagau cgcggcugaa auggcccaua aguccagguu cggcaaaguc     1080

uggcugggua ugucagcaga ggcccaggcu caagugauag aacgccuccg ggggcuggag     1140

agugaugccg acgcagauga cuuugaggcu uggcuuagag acgaucacgg acuggcccag     1200

gaacaggcga aggccguaac cgcugcaaac uugcccgagg gcuacggcag guucggggaa     1260

acggccacuc gaaaacucau ugcugcccug gagcaagagg uuaucgugua cagcgaagca     1320

guccagcgcg cgggauuagg ucaucacucu gaccugcggg auggccaggu guggaccgac     1380

aagaaaggga auccugcccu accauauuac ggagcugugc ucgagagaca cauugucccc     1440

ggcacagccg auccgaacga aacugacgau gcacugagga ucggucgucu uaccaauccu     1500

acaguucaua ucggguugaa ccagcugcgc cgggugguaa augcucucau gagaagguau     1560

ggccaacccc aggagauugc cauagaacug gcacgagagc uuaagcuguc cgacgaucgg     1620

aagaaagaga ucaacacccg caacagagaa aauaggaagg ccgcugagaa acggucacag     1680

aaguugcgaa acauuggaca ggcggacaaa ggcgggaaua gagcccuccu gaaguuaugg     1740

gaagaacuga acccagaaaa ugugcuagau agaaggugug ucuacacggg acgugugauc     1800

agcauagaca ugcucuuuag uggugcagcu gagguugauc acauucugcc uuucucugug     1860

ucccuugacg acagcaacgg caaucggauc uugugccuga gagaggucaa caggaucaag     1920

cgccaacgaa cucccuggga agccagagau gaccuccggg cccauuuugg gccagaugcu     1980

ggaugggagg caauugccgc gagggcugcc cgccugcccc gugaaaaacg cuggcgguuc     2040

gagccugacg cagccaauag guuugaugcu cagggcgguu ucuuagcacg ccaccugaca     2100

gacacccagc aucuuucgcg auuggccaga ucuuaucucu ccgcgcugua cccagaaacu     2160

ggggagggcu caggacacgu guggguaagu ccgggccggc uaacagagau gcugaggcgc     2220

gcuuggagcc ucaaccacca ucugcccgau cacaauaucg ccggggcagc caaccagccu     2280

aagaauagaa aagaccauag gcaccacgcu auugaugcca uaguuguggc acuugcugac     2340

cgggccaugu ugaaccguau cgcgcgcgaa uccggaaagg agggucauga agccgcaggc     2400

cgacuggucg cuucuauucc agagcccugg gagggguuua gcgaugaagu ggguagagcc     2460

gucagggcua ucaccguguc acaccggccu gaccauggca cggcaaguaa agccggucgc     2520

gccaaggaua aggacgcuac cgcggggaga uuacacaaug agacagcaua uggccucacu     2580

ggagaaaaag acgccagggg ccuggauauc guugugaccc ggaagccacu uuccgcuuuc     2640

aaaaagagcg ccgaccugga ugcaauucga gacgaugacu ugaaacaaaa gcucaaagcc     2700

ugggcugcgg ggaaggaggg aggcgccuuu gaacaggcaa ugagaucuuu cggugauccc     2760

gagcgcgggc cgaggucaca uccuggacug cggcguauaa gaguaacaga gccacuaagu     2820

gucaucccca uuagggaccg cgauggccgg ccuuacaagg cuuacaaagg ugacagcaac     2880

uaucgauuug auaucuggga acugaagacu ggcaaaugga aggacgaggu gguuuccaug     2940

uucgaagccc acagacccaa cuggaccucu ccaauuaggg cagggaaucc uacagcccgc     3000

aaagugcucu cgguccggca gaacgacgug aucgcuauag agagagaaaa uggacccgag     3060

cugaugcgug uagugaaguu cagcgccggc ucccuugcgu uggcaccacc gcaggaggcc     3120

gggucacuga aggcuaggga ugccgacaaa aacgauccuu uuaaguacgu uuauggaagu     3180

cccucuagcu uacaacggug gaaagcucgc cagguccgaa ucgacgaacu cggcagagug     3240

uucgauccag guuuugcagu gaggucccgg cgcucaagcg aguga                     3285


<210>  2746
<211>  3291
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2746
augaagaaca ucuucagcuu ugacauuggc accaauucca uagggugggc cgugcuggcu       60

cucgauggaa acggugaccc cgucagaauc gcagaugccg gcguuaggau uuucucugac      120

gggcgggauc cuaaaucaaa ggcgagucuu gcugaggccc gccgaauggc acgugccaug      180

agcagaaggc gggaccgcac acugagaagg aaacgggcua cuuugcgcac guuagcagaa      240

aucggacuga ugccagauac cuccucugcc cgacaggcuc aacuaagaga gacaucggac      300

ccgaagucag gcagcgguca gcucacuagg aaucccuacg cccugcgggc gcuugcacug      360

gaagagaaau ugccucucuu ucacauuggg cgugcccugu aucaucuggg acagcgcaga      420

ggcuuccaga guaacaggaa gaccgaucgg aaggacaaug auaaaggcaa gaucgcucuc      480

gggauagaag aguuacacgc cgcaaugcga caagacggag cucagacaua cggucagugg      540

cuggcccgca gacaugcgcu uggccaggcc gugagguugc gguccggggc acuggaucua      600

gagggagaag gcuaugcuuu uuacccagag cgcaaacucc uggaagacga guuccacaga      660

aucugggagg cccaagcuag gcauuauccc gacauucuua ccccugaacg ucgggaucac      720

cuguuuacug uaauguucuu ucagcgacca uugaagaaac ccaagguggg uaagugcucu      780

uucaacaauc aggaggaaag acucagcaaa gcccacccuc uguuucagga guucagguua      840

uacaaggaag ucaacgagcu ggagaucgug gaaccagacc ucacacauaa aaagcugacc      900

cuggaucaac gcaaugcauu gguuaaaaag cuacaggugu cccgggaggc cucauuugcu      960

gcgcugagaa aaguccucaa gcugaggccg gaccagcgcc uuaacaagga aagugagagc     1020

cgaacgaaac ugaaggggaa ugaaguguau gcaguauugu cugauaaaac ucgguucgga     1080

ccccguuggg gcaccuucuc ccaagccgag cagugggcua uuaucgagag acucaaggaa     1140

gaggaagacc cuggcaggcu guuugccugg uuacgcucag cauucgggau agagggagaa     1200

caggccgcug auauugccaa cguucaccuu ccagaggguc auggccggcu agggaaaaca     1260

gcacucgagg cgaugcugga ggaacuuaag gcugaaguga ucacugaggc cgaggcagcc     1320

aagagaugug gauacgacca cgcuagguug ggcgguggga gcgaugaagg cgagcccgaa     1380

cugccuccau accaggagau ucuccaacac cgaaucccgc ccggaaccaa uaacccugac     1440

gaucccuaug acaucgaaaa aggccgcuuu acaaauccaa ccguccauau ugcccugaac     1500

cagcuucggc acgugaugaa cacgcugaua agaagguacg ggaagccugc acagaucgcu     1560

guggaguugg cccgugaccu caaacugagu gagcgccaga aggaagaggu caaucggacu     1620

auugcgagaa acacaaggga ugccgaacga cggggaaaaa ccuuagagga aaaucgcauc     1680

cccaacacug guuauaauag aacacugcua aggcucuggg aggagcugaa cccaggcaag     1740

guugaagacc ggugcuguau auacaccggg aagcccauuu cuaucuccau gcuuuucagc     1800

ggagaggugg auaucgacca uauuuugccu uggucgcgaa cgcuggauga cucucacggc     1860

aauaaacucc ugugcuuaag agaagcuaac cgcguaaaga ggaaucgugc accagccgau     1920

gugaccgagu ggcgggacaa cuaugaugaa auccugucca gagcugccag gcuuccgcaa     1980

aauaaacgcg accgauuugc acccgaugcg auggagagau ucgagaaggc cgguggguuu     2040

uuggcucggc agcucacaga cacucaguac cugucaagga uggcccuaga auaucuggca     2100

ugucucuucc cugcugagga aauggauggc uuuggagagc ugcgccguca uagauacauu     2160

aacgucugcc caggccggcu uaccgagaug uugaggcgca auugggggcu gaacaguaua     2220

uuaagcgacu cccagcucgg agaaacagcc caagagaaaa aucgaaagga ccacagacac     2280

caugcaaucg augccauugu gaucgguguu acuggccggu cucugcuuaa caggaucagc     2340

uacgcugcgg ggcgccugga agcccagcag uuggagaccc ucgugaagga agcagcugcc     2400

acaaacaaac ccugggacgg auucagagag gaugucgcca aggugguagg cgguauuaua     2460

guuucacaca aaccugacca ugggaccgcu ggcaggaagg caaaagaucc agcccggcag     2520

caaacugcgg gacgucugca caaugacacg gcuuauggcc uaacagggga gaccgaugcc     2580

cgcggaaaca cuaucguggu ccgaagaaag cccuuucuga gucucaccuc caaagacauu     2640

ccgagcauca gggaugaagc acugcgggcc gugcuugagc agcacuugcg cggcauuccu     2700

gacgguaagg cuuuccaggc agcccuggau gcguuuagaa agcaaaaagg guuccaucac     2760

uaccauggaa ucaggcacgu ccgguuaaca gaaccacucu cugugauagg caucaaggac     2820

cgagauggua aaguuuauaa gggcuacaaa ggggacgcua aucagagaua ugacgugugg     2880

gagacccugg auggaaagug gcaugccgaa guagucucaa uguucgacgc acaccagccc     2940

ggcuggcaga gucgcuuuca cgccgagaac ccuacugcua ggcgggugcu ucgucugcaa     3000

caggaugaca ugguugcccu aaaugcaccc gaugggggaa gacuccuggu gagggucgug     3060

aaguuccgcc agaacggcca gauuacauuu gcuggucaua augagagcgg gaacuugaaa     3120

gcccgggacg cggccuccaa ugaacaagau ccauucaagu acaucucucg aauggcaucg     3180

agccugaaag acuguggaug cagacaggua aggauugaug cuacgggcca gguguuugac     3240

ccuggucccc aggaucgcgc cucccgguua caaaagcagu caaaaagaug a              3291


<210>  2747
<211>  3294
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2747
augaagaaac agccccaaag auacauccug ggacucgacc uugggauugc cagcguggga       60

ugggcucuga uauuggucga uggugcagag aacccuguug gcuuacugga cugcggggug      120

aggaccuucg aacgggccga gguaccaaag acaggagauu cccuagcgcu cgcucgccga      180

caggcccguu cugugagaag gcugauccgg cgcagagcac acaggcuucu gcgguugcgc      240

cgacuccuga aaagagaagg cauucuguca gccgcugacu uuaauaguga uggucucccg      300

aacuuaccca ucgacgcaug gcagcugagg gucgccgggc uugagcggaa guugacuaau      360

caggaauggg cugccgugcu gcuacaucuc guuaaacacc guggauaucu gagccaacgc      420

aaguccgaga cgcagaccga gaacaaggaa cuuggcagac uguuggcggg cguggcagaa      480

aauucuaaac uccugucgga aucaagcgag caguacagga cuccugccga guuagcuguc      540

aagcuguucg ccgaacagag ugggcaucuc cggaacaaag gaggugagua uacacacacc      600

uuuaaucgac uugaucugca aaaggaauug caucuacugu ucgagcgcca guacgcacuc      660

ggcaacccau uugcuuccgc cgaacugcag ucuaaagugg acgagcuucu gaugacacag      720

agaagcgcgu ugcaagggga ggccguacuc aagaugcugg gaaaguguac cuucgaaccc      780

uccgaguaua aagcagcuaa gaauacuuac ucagccgaaa gguuuauuug guuaacaaaa      840

cugaacaauc uacggauccu cuauaacggc gaggaacgcg cucuggauga gggugacaga      900

aagaaacuuu uggaugagcc uuacaagaaa gccaagcuga ccuacaagca gguuaggagu      960

auacucaauc ugccagaaag cgcaguguuc aaagggcuuc guuauggaaa ggacucugau     1020

ggccugaaag ccgagaacgg cacguugaug gaaaugaagg cuuaccacaa aauccggacu     1080

auucucgaca aggcggggcu gaagggagag ugggaaaauc gaaucagaaa ccccgagauu     1140

uuagacaaaa ucgguaccgc auuuucccug uauaagacag augaggacau acaggccgcu     1200

cuagccgauc ucaggcugcc ugaaaacguc cuugacgcau ugcuggccgg ccucaauuuc     1260

gauaaauuua uucaacuguc auuaaaggcu cugcgcaaca uccuuccauu gauggagcgg     1320

gggauuagau acgacgaagc cugcaaagag guguauggag aucacagggc acucgcggag     1380

agcaagaauc aggugcugcu accgcccauc ccugcugacg aaauccgcaa cccagucguu     1440

cugcgaacuc ucacccaggc ccggaaggug auuaaugcaa uaauccguag auacggcagu     1500

ccccagaggg uacauauuga gacagcccgc gaacugggua aaucuaagca agagcgggau     1560

aaaaucgaca gaaagaacaa agaaaaucag aacgauagga aucgacuugc uuccgacaua     1620

cgcaagcacu ucaacuuuga gccuacccca aaggacauuu ugaaacugcg guuauaugag     1680

cagcagcaug ggaagugucu cuacagcgau cugagagaag agaaaggcga augccuuuac     1740

acgggaaaug ugaucgagcu gcacagguug uucgaagacg gcuaugucga gaucgaucac     1800

gcccuccccc ugucgcguac uugggacaac ucucuaaaua acaaggugcu gguucucaaa     1860

uccucaaauc aaaacaaggg gaacaaaaca ccguacgagu ggcuggaugg agcaaauaac     1920

agucgccggu ggagagaguu ccaggcuagg gugcaggguu gucauuuucc uuaugucaag     1980

aagcagcgga uucaaaccgc caaacuugac agcaaggcgg agcagggcuu cuuggaacga     2040

aaucugaacg auacucgcua caucuccaaa uuuaugugcc aguucauuac agccaauuua     2100

agacucgcug agaccucuaa cgcacugaug gaagaggugg ccggggagaa ucccuauugg     2160

aaggaaagga aacggcagcg aguauuugcu ccaagcggau caauaaucgc ccuucugaga     2220

ggccuauggg gucucaagaa gguucgcgag gacaacgaua ggcaccaugc acuggacgcg     2280

auuguggucg ccuguaguac gguguccaug caacagaaaa ucaccaaggc uaugcagcgu     2340

cgggaaacau uggagacugu cgauagcgag accggggaag ugaaaacaag aaucccucaa     2400

cccugggacu ucuuuaggca ggagguuaug auucgcgugu ucucugauuc accacgagag     2460

gaacuggccg aaaaguuaaa uaguagaccc gaggcacucc acgacuuugu aagcccucug     2520

uucgucuccc gggcuccaaa caggaaaaug acuggccagg gacaucuuga gaccgugaag     2580

ucugccaagc gccugucgga aaauauaagc guugugcgua aaccguugac agcacucaag     2640

cugaaagaca ucgagaacau ugucggcuac cccgauagag aaccugcccu auaucaggcu     2700

cugcgggaga ggcuucucgc gcacaaggac gauccaguga aagccuuugc agacaagccc     2760

uucuacaagc cugcuaaaga ugggggacaa gguucccugg uaaaggccgu gcgcaucauu     2820

gaaaauacca aacacggcuu gaagauccga aacgggauaa cugacacgaa uggagauaug     2880

guuagaacag acgucuacca gaaaaccgau aagaaaggca agcggcagua uuuuaucgug     2940

ccaguguacg ccuggcaggu cgagaagaac gagcugccca cuguugacug caaagguuau     3000

gaaauugaug acaacuacga guucuguuuc ucauuauaua aguacgaucu caucgaaauu     3060

caaaauccga acgacaucaa ucaggagcug agggcuuauu acauaaacug cgacaguucu     3120

gaugggcgcu uuuggcuugc auaccaugac aaaagcgcca aggaacagca guucagaauu     3180

uccacccaaa aucagccuau cauuaaaaag uaucaggugg auacacuggg caaggagauc     3240

agguugugua aagcggagaa gcggaccgcu cucaacggca aaaagaaaaa guga           3294


<210>  2748
<211>  3294
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2748
augggcaagg gggacauccu guucagcuuu gauauuggaa ccaacuccau agguuggugc       60

guguucgccc ucucugacaa uggcgauccc uguagaaucc uugacgcuaa cgcaaggauu      120

uuugccgaug ggcgggagcc uaaaucagga aauagucugg cggaaggccg ccgagcugcc      180

cgugcaaugg ccagaaggcg ggaccgcuac aagagaaggc ggaaagcugu cuugcgcaca      240

uuaacugagu auggucugau gccagcagau ccgaaggccc gacaggcucu aguugccaaa      300

acgggggaca gaggacccaa gagcgaagag gcgcucgcag auguguacuc ccugagggcc      360

cgggcucuua uggaaaagcu gccuuugcac cauaucggcc gugcccucuu ccaccugaac      420

caacgcagag gcuuuaaauc uaauaggaag accgaccgga aaucgaacga gaagggggca      480

auugagacag gaguaaaucg acugcucgaa gagaugcgcg auacuggugc uccaaccuua      540

ggcgccuauc uggcgaaaag aaggucagac gggcggcccg ugcgcgucag agccggagca      600

agcgaacagg aggcugagga uggcaagccu gccaagacag cuuacgacuu cuauccagug      660

aggcgucuuu uggaagagga auuugacgcc aucugggaga aacaguacag uuaucauccc      720

gaaguucuga ccgaggagcg gcgaggucac cuauucagag ugauguuuca ucagaggccu      780

cucaagccag caauagucgg gcgcugcucc uucaacucug ccgaaacucg gcuggcuaaa      840

gcgcacccgc uuuuucaaga guucagacug uacaaggaag ugaaugaguu ggaaguaguu      900

augcccgauc agagccacag gaaacucaca cugccugagc gcgaccgauu agugcugaag      960

cuccggggac agcguaaagc aaccuacgcc gcucuuagaa aggagcugag guugcgcgau     1020

cuacgguuua acaaggaaac ggagaauaga acugacauga ugggcgauga agucaccgcc     1080

gcacuggccg acaaagcugu guucggcaac aggugguccg gguucucaac agaugagaag     1140

uggcgaaucg uggaacgccu ccgggaggag gaagacccau uugcccugca ugaguggcuu     1200

gucagugaac acggacugag cgaugcagag gcggagcagg cugccaaagu ucauuugccc     1260

gacgguuaug gcagacucgg gccuacugca gugucuacca ugcuggaaga guuaaaggcc     1320

gauguaauua cagaagcuga cgccgcaagg cuggcuggau acgaccacuc ccuagcccgu     1380

gcggaugagg acggccucga ucgccugcca aaauaucaag aagugcuuga gcggagaauc     1440

ccgcccggua ccaaugaccc ugaugacccc uacgauauug ccaaggggag gaucacgaac     1500

ccaacugucc acauagcuuu gaaucaguuc cgacaugugg uuaacgcacu ggcccggcgc     1560

uuuggcaagc cugaccagau ugcuaucgag cucgccagag aacugaaacu uaacgauagg     1620

cagaaggacg aggugaaucg ggucauugga cgaaacacaa gagcagcggc ccugcgcuca     1680

caaaaauuga gggauaucgg ccagaaggac aauggguaua accgugcucu ccugaaauua     1740

ugggaagaac ugaaucccga uaagccagaa gaccgggugu guguauacac cggaagaccc     1800

aucugcauua gcaugcuauu caguggugag guugacgugg aucacauacu cccuuggucu     1860

aggacuaugg acgauuccca ggccaaccgc auccugugug ucguggaggc aaaucgaguc     1920

aaggcuaaca gagccccagc agaaguggcc gaguggggcg ggcgguauga cgaaauucuu     1980

gagagggcug cgcgcuugcc gcguaauaaa agauggcggu uugccaggga ugcaauggaa     2040

aaguacaaag agaacgaggg auuccuggcu cgccaacuca cagacaccca guaccugagc     2100

cgauuagcca gagaauaugc cgaggcucug uaccccacgg aggagccuga ucggcuuggc     2160

gaguugaggc gcagaaauca uguuagggug aucccagguc ggcucaccga aaugcugcgu     2220

cgcggguggg gccuaaacgc acugcucccc gaccacaauu uuggagccgg ccaggcgaag     2280

aaccgaaaag aucauagaca ccacgcuaua gacgccgcag uagucggggu gacaacuagg     2340

ggacugcuuc agcggauuuc gaccgaggcc gguuugcgcg aaaagcuggc uuuagaggaa     2400

acaaucgcag auaucccuau gccauggccc ggcuucagag aggagcucaa ggccgcgauu     2460

gacagggcug uugugucuca uaaaccggau cacgggacuc ugccuaagcc aggagaacau     2520

ggccggaccg ccggucaacu ucacaaugac acagcauaug ggaucaccgg cgagacugac     2580

gcccgaggaa accccauugu cgugagacgc aaaccuuuuc uguccuugac ggauaaggac     2640

auacccucaa ucagggauac agaacuccgg ggccguguau acagugcuac cagcgggcug     2700

uccggaagag agcuaucugc cgcacuggac uauuucagga aaacugauaa gcaguuucgc     2760

ggcauucggc acgugcgagu uaccgaaagc cucucaguca ucccaaucag agacagggau     2820

gguaacauuu acaaagggua uaagggagac gcuaauuucc gcuacgaugu gugggagcug     2880

ccugacggca aguggaaagc cgaugugguc acaauguuug acgcgcaucg gcccgacaga     2940

gccgcagagg aacguaggcc acacccggcu gcccggaagg uucuuaguuu gaaacagaac     3000

gauauggugg cuugcgagca uccuaaggac gguuacacca uaggccgcgu gguaaaauuc     3060

aauacugcag ggcagaucua uuucgccgcc cacaacgaag gaggcucccu gaaggcucga     3120

gaugcggaca gagcagaucc cuuuaaguac uucagcaaau cugccucagg guuaaaggag     3180

auugcugcca ggcaaguccg gaucgacgca acaggacagg ugcucgaucc aggcccccag     3240

gaccgcgaaa guagacuggc caggaauaaa cgacuugagc gggagcgugc uuga           3294


<210>  2749
<211>  3303
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2749
augaaguaca ccaucggccu ggacauuggg acaagcuccg ugggaugggg ugucauagau       60

acugagagaa acaggaucca ggaccucggc guucggcuuu ucgaaucugc cgagaaucaa      120

aaagaugggg aaucacuggc uaagccccgc cgagacgcac guaguaugag aaggcgguug      180

gcccgcagag gaagcagguu agagcgggug aaauccauuu uugaaaaggc gaaauuccug      240

acgcgcggcc agauggguau cacccacgcu cgaccuaacg auccauauag acuaagggcc      300

gaggggcucg accggcugcu uucuaaugag gaucuguuua uugcaguaua ccauuuggcc      360

aagcgucgcg gauauaaguc gaacagaaag aaugugcucg aaggcgacgg cgaggauguc      420

agggaaggga aggcugugcu ggacggaguu gcacagaaca aaaagcugau ggccuuccgg      480

gguuaccgaa cagugggcga gauguuuuuc aaagauucag ucuuuacuga ggcuaagcgc      540

aauaaggccg ggagcuauaa acacugcgug cucagagaaa uguuagagga cgaacuggga      600

aagauccuuc augcgcagag gaguuugggc aacacccugg uaacagauuc cuucauaucu      660

aaacuacucg agaagaaaac cgguguuuuu guguaccaac ggccguucgc aagcgggaau      720

cagauccuga aacuugucgg acccuguacu uuugaaccug aggagauucg cgccgcuaaa      780

gccacauauu ccuuccagua cuuuaacgug cugcagagau ugaauaaccu caccaucaag      840

aauacggaca cuggcaccga aaggaagcug acaucagagg aacgugcaaa aaugauugcu      900

uucaucuuca gucacaagga cgugcaauac gcccagauac gguuagagcu gaaacucagc      960

gaugucgacc gauuuaacau gguuaauuau uucgugcaga gaaaggaacu ugagacuaaa     1020

accgcggagg aacugaggcg cgagauugaa aagaagacaa aauuuccauc uuuguccgau     1080

uacuauacca uccggaagac gguaucagag auugacagcg ccuucuggau ccagaucgaa     1140

aaacaacaug augugauuga cgcaauaggc gagaucuuua cucuauacaa gacagaugag     1200

gacauuaucg cucagcugaa caccagaaua cccaguauuc cuaggccagu cgugucugcc     1260

cuccuggggc uuuccuucac uaaauuugga caccugagcu ugaaggcucu ccgcaagauc     1320

aucccccacc uggaaaacgg ugauacauau gacguugccu gcgcaaaagc cgauccuaag     1380

uacaccgacc gagcuaaaac gcgggcgcgu aaguuaagac cacuggacaa agaggaucau     1440

ucgauuacca auccgguggc aaggcgcgcc aucgcucaga caauuaaggu cgugaacgcc     1500

auaaucgaca aguaugauuc ucccuccgaa guacacauug agcuaggccg ggaccucgcc     1560

aaaaauuuca aggagagaau gaaaauccaa aaggaacaga aacagaacca ggaggggaau     1620

gaaagggcaa ucgcuaagau ucaugagcug uacaaacuug aaaacccucg aggacaagau     1680

auaaucaagu uucgcuugug gaaugagcag aacggccagu guauguacuc aggugagcgg     1740

auugacgaaa ucagacuguu cgauaauggg uauacugaga uugaccacau cauaccauuu     1800

aguaggagcu ucaacgauuc cuacucuaau aagguucucg ugcugaaaau gcguaaccag     1860

gaaaagauga auaaaacccc cuaugaguac augggcggag acgccgauac auggcaacgc     1920

uuugaaaacc uugucaauag caugcacaac aucggcccuc ggaagaaaca gaaccuguug     1980

cucaagaagu auguggcaga cgaucugacu uuaagaaccc ugaaugacac aagguucauu     2040

gcgcgauaca ugaaaaacua uaucgagaau acccuacucu ucacugaggg gccacagaag     2100

cagcgggucu acacggugaa cggacaagcu acagccuacc ugcgcaaaag auggguucuu     2160

cauaaggaca ggaccgaaaa uaaccggcac caugcacagg augccguggu agucgcugug     2220

gccacucccu cauugguuca ggguauuaug cgacacgcaa aaaugggcga gaucguggac     2280

uaucugcaua gucagaaguu ugcuccgaau gucaccgaua uagaaacagg gaagccuuuc     2340

gaguccgccg acauucaaag cgcgaaagaa agaaucauug ccaagcacga gcaccgccuc     2400

aggauccccg agccauggga aggauuuucu gaugagcugg acgcucguau gggcgaugac     2460

gugguagaaa aauuaucacu gcggaagagu aacauacaug guuacaaaac cacuagcgau     2520

uucucccaga uccacccuau uuuugugucu agaaugccca agaggaagac aacggggcgc     2580

gcacaucagg agacccuucg aucgccaaga cgguucaaag aguuucaaga aagcguuguc     2640

aggacucccu uguccucaau caaucucaag aaacuggagg aaaugagugg caagaacucu     2700

gacaaacuac uguaugauau ucuccgcaag cgucugcuug cccacggaga cgauccuaaa     2760

aaggcuuuca cagagccagu gcacaagccg agaaaagacg gcacccaggg gcccuuggug     2820

cggagcauca agauggucuc cgaaggacag aggucaggua uacugguuaa uaacgagcag     2880

gccuuagugg accaagauag caugauucgc aucgacaucu uuucuaaacc uaauaagaaa     2940

ggcaaggaug aguacuauau uaucccagug uacgcauauc auuucgcgca gaaggaacuc     3000

cccaacaaag uaauuacagc caaggcugag gccgacuggc gaacuauaga ugacaccuuu     3060

gcauucgaau uuucccugua cccuaaugau cuugucaaac ugacgaaggg ggacgauauc     3120

agauucggau acuauagugg cugcaaccgg gcuuuggccg caauucacau ccucccacau     3180

gaccagaaca ugccccuggu gaauaacggu auuaaaaccc uagauaagau cgacaaguac     3240

guuguggaca uacuggggga guaucacaaa gucggcaagg aaacaaggca gcaaauccag     3300

uga                                                                   3303


<210>  2750
<211>  3303
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2750
augggcgcca ccaucgugac agacauuagc agauccgcuu ucgcauuuga uauagggacu       60

aagucuaucg gaucagccgu ccugaggcuc gacgaucggg gugagcccau uggcaucauu      120

aacgcggcug uucgcaucua cgccgacggg cgagaaccua aaaguggaac gagccuugca      180

gagggccgua gacuggccag ggguaugucc cggcgcagag auagguauaa gcggcgccga      240

agaaccuugu uaaauacacu gacugaguuc gggcuaaugc caccgaccga cgaggcuagg      300

aaagcacucc uggccgaaac aaacgaucgg cguuuuggag cuguggccgg caauguauac      360

gcgcuucgcg caagagcccu ggacgagaag uugccccucc accaucuggg cagggcucug      420

uuccaccuca accagcggcg aggguuuaaa ucuaaucgca agaccgauaa gagagacaac      480

gaucaaggaa aaauagccuc ggguaucuca agguuaggcc agaagcugga cgaggcaggg      540

gcucggacuc uuggagaguu cuugcaucgc cagcacagcg ccggcaaaug ggugagaguc      600

aggccuaguc gugugccauc caaggaugac aaagaagaag acgguuaugg guuuuacccc      660

gagcggucuu uccuggagcg agaauuugcg gccauuugga aggcacaggc ugccuucuau      720

ccugagcuac ucacagaaga gagaggagau caucugcaca ggguuauguu uuuccaacgc      780

ccacuuaagc ggcccaaagu gggcaagugc agcuuuuacc cugacgaaga uagacugcca      840

aaagcucacc cguuguucca ggaguucagg cucgucaagg agcugaauga auuagaggug      900

guacaucgcg gcgaccgacg ggaaaaacug acccucgagc agcgugauac gcuuuuuaac      960

cagcugagaa cucaaagguc cucagccuuc accgcauugc gcaagacacu aaaagccccc     1020

acugggaccc gguuuaauaa ggaaagugac gcuagagaua agcugaaagg agacgagguu     1080

gcggcagaga ugagccacaa ggauagguuc ggucaggccu ggaucggcau gccucucgcu     1140

gaacgauggg ccgugauuga gaugcugcuu gacacagaag auccacugga ggucaaagca     1200

ugguuggaag aacauuaugg guuugccgcu gaccgcaucg acgccauagc agcggcuaag     1260

cuacccccug gauacggccg gcugggugag accgccuuaa gagauaugcu ggacgaacuc     1320

aaaaacggga cggugaagga ugacucuggc agggaugugg uccucacuga gcgucaggca     1380

auggacaaua uuuggaagga uaacccagcc cugcagcgcg uuguggcuga cgcccgggaa     1440

cuucccccgu accaagaggu auugggaaga aggaucccuc ccggcacagg gaaaccagag     1500

gauccuuaug acgugcagaa gggacgacug accaauccca cuguccacau uggucucaac     1560

cagcugaaac gggugguuaa ugcacuugug cgcagacacg gcaggccaga ucaaaucgcu     1620

cuggaauugg cccgggagcu caagcugaac gaggaacaga aagcggaaaa ggaccgagag     1680

aucgccagaa acacaaaggc ugcacaggcc cgcuccgaga aauuaaggca gcuggauauu     1740

gacgacaaug gguacaaccg uguccuacuc aagcuguggg aagaucuuaa uuugcggcaa     1800

cccgagaaca gagaauguau auauugcgga aaagcuaucg gcauugguac ccuguucuca     1860

aaucaggugg acaucgauca uauacucccu uggagcaaga cgcuggacga uagucaggcc     1920

aacaggauug uaugucacgu ugagugcaau cgccagaaag ggaaccaugc accagcggaa     1980

gugccgcaau ggcacgaccg auacgaugac aucuuagcca gagcugagcg gcugcccagg     2040

aauaaggccu ggcgcuuugg ccgugaggca augaagaaau ucaaggauga cgaaucuuuu     2100

cuugcuagac aguugaccga uacacaguau cucucccgga uggcccauga guacgucgca     2160

gcccuguauc cugacgagga accagaugaa uggggagugu ucacuaggcg caaccacguc     2220

cgagugaucc ccggcagaau gaccgagaug cuacggaggc agugggggcu gaaugcuauu     2280

aucagcgacc acgaccagaa aaaccgcaau gaucauagac accaugcgau ugacgccaua     2340

guugugggag uaacaacuag gcggcuccug caacguaucg caaccgcugc cgguggccgc     2400

gaggcccuug cuuuggaaga gacaauuggg aagaucgaac cuccauggcc cggauuuuac     2460

gaugacguca aagaggcaau cgaucgaauu gugguuucgc acaagccgga ccacggcucu     2520

cugccuaaac caggugagcc cgccagaacc gcggggcagu uacauaacga uacugcuuac     2580

ggcuucacgg gagaagagag gaagggcgug ccuauaaugg uccaccgggu gcccuuugcc     2640

acacucaccg aaaaggacau cccauccauu cgcgauagac agcuggcaga ggcccuuuau     2700

ggggcuaucg caggaauuuc aggcaaagcc gaacuggcgg cugccuuggc aagguucagu     2760

gccagcgguc cuuacaaggg gauccggcau guacgagugg uugagcccgu cuccgugaua     2820

gcuaucaaag acagagaugg aacuccauau aagggcuaca aaggugacgc caauuaucgc     2880

uacgacgugu gggagcuccc gaacggcaag uggacccacg aagucguuuc uauguucgau     2940

gcacauaggc cugacuggca aagcgagauu cggaacaaga aucccgcugc ccguaaagug     3000

cugucacuac accagaacga uaugguggcg uaugaacacc cacaggacgg guacacaauc     3060

ggaagaguag ucaaguuugg caguggggga caaauggccc uggcaggcaa uagggagggu     3120

ggggcucuca aaucccgcga ugccgacccc gaagaucggu uccgauacuu uuauaagagc     3180

ggaggcgcua ugagagacgc aaggcugcgc caggugcggg uugaugagac cgguagagug     3240

uucgacccug gcccacagga ucgugagucu aggaacgccc ggaaacaguc agccgcuggg     3300

uga                                                                   3303


<210>  2751
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2751
augaagaccc ugggccucga ccuugggaca aacagccugg gaugggccau cguggagacu       60

agagaugaac ccacgaaaua cuccgagggu ucuauugucg cuucaggcgu uaggauauuc      120

agucagagcg aaauggcagg gcgggacccu caauccaagg ccucuuuggc gguggcucgc      180

cgacaggccc guggaucgag aaggcggcgc gauagauauu uaaaaaggcg gcagcgccug      240

guagaccuac ucguggaucu gggccuuaug ccagcagaca aggccgcucg agcagagcug      300

gucaaaaauc acggugaugg gaagaccgga gacuugucaa gcagugugua cgcccucaga      360

gcuagggccc uggaugaagc gcuguccccg uaugagaucg gccgggcaau uuuucaucuc      420

aaccagcguc gcggcuucaa gucuaauaga aaagccgaca gcaacgaucc cgagcaaggg      480

guuaucuccg aaggaaugag ggcuuuagac gagaagaugg ccgaagcugg ugcacggaca      540

auuggcgagu ggcugcacau gcgacgccuu cagggguugc cugugagagc caggaucgcg      600

ccagauggag agggcuacgc cuuuuauccc ucacggggug cacuggaacg cgaguucgac      660

agaauaaugg cuaggcagac ucuauuucau accgaccucc ugacagauac cgccauccgu      720

gaacuucggg aggucguguu ccaccagcga ccucugagac caguaaaacc cgggaagugc      780

aguuacaauc cuuuggaaga gaggcucgcu aaagcccauc cacuguuuca aaaguuccgc      840

uuacugaagg agcucaacga acuugagauu guuggagaag accagaaaca caucaagcug      900

acuccggagc agcggaaugc auuggcccua cagcugagaa caagccucac caaacaaggc      960

aaggugcccu uuucuaaacu gagguccacg cuuaagcugg gccgcgaagu ccgauucaac     1020

aaagagacug auaaucgguc agacuuggag ggggauguga uuuauuuucg uaugagcaga     1080

ccugaauguu ucggaaacag gugggcugac cucccagugg agaugcaggc ggcaaucacc     1140

gaacgccugc ggacagagcc cgauuacaag acuuuaauga auuggcugaa ggccgaaacc     1200

ggucuagacg agguccacgc uagagccguu gcagauacac cugugccaga cggcuuuggg     1260

aggcucggac agagugcccu gaccgcucuu auagaugccu uggagaacga aguaggcgag     1320

gguggguucg ugauuacgga agcagaggcg gcuaaacgag ucuacggccg cacuaauucu     1380

gaggccgacc ccgaccggaa gggaguggau gaacugccga aauaucaaga gguucucgca     1440

agacauaucc cucccggcac aggggaacca gacgauccuu acgacgauuu caugggaagg     1500

auuaccaacc ccacugugca caucgcccug aaucagcuuc gucgcgucgu gaacgcucug     1560

auccggacau augguaagcc agacagaauu gccauagagg uaggcaggga uuugaaaaug     1620

aacgaccgag aacgggauga gguuaaucgc agaaucggga ggaacaccaa ggcagcuauu     1680

gagcggggag ugaagcucac ggaaaccuuu cgacagcugg acacaggcua caauagauua     1740

cgccuggagc uaugggaaga ucucaaccag aaacccgaga auagggucug caucuauugu     1800

gguaaggcca uagcggggca uaugcuguuc aacggcgaaa cugacauuga ucacauccuu     1860

ccuuacucca aaaccuugga cgacagcaag gccaaucgua uccugugcug uacaucgugc     1920

aaccgggaga aaaagaauag accaccgucu agggugcccc aauggcgcuc ccgauaugga     1980

gagauucucg cuagagcaac uacccugccu aagaacaaac aguggcgguu ugccgaagau     2040

gcuaugacaa gguucggcga ggaagagggg uuuuuagccc gccagcugac cgacaugcag     2100

uacaucucac gucuugcauu gacuuaccuc gcgcaccugu augauuacga agaggucgac     2160

cuagauggaa uuuauaagag acaugcccgg gugagggcuc ugccaggucg caugacggag     2220

augcuccgaa gacaaugggg ccugaaugaa cuuuugcacg accauaaccu gauaggggga     2280

gauguugcca aacccaagaa ucgguuagac cacaggcauc acgcaaucga ugcuauugug     2340

aucgccugua caagucgcgc acucauccag agacuggcca ccgcuagcgc ggccgcagag     2400

gaaaggggcg cugagcgggu agucgagcgu auugacccuc caugguccgg uuuccgcgaa     2460

gaugugcgag ccgccguugg ggcuauagug gucucucaca aacccgacca uggcacugug     2520

ggaagagagg gcuacgcaaa ggggagggga cagaccgccg gcaaacuuca caacgauaca     2580

gcguaugguc auaccgggga aaaggacgcu agcggaaaug acaucguagu gcggcgcauu     2640

gccguuacug auaucagauc accggcagac auuaggaaga uccggacaaa cgcccacggc     2700

cacagugagc ugcgagauag auuguacgaa gcuacgcgcg gucucgaggg caaagcauuu     2760

gagcaggccg uccuggacuu cgugaagggg gaugcgaaau uuaggggaau acggcaugug     2820

cguaucaccg aagucgcuaa cccuguuugg auuacucacg gcgacgggag acauaagaaa     2880

ggauaucuac caggcgguaa ugauagguuc gacguguggg agcuggccga ugggcgcugg     2940

gacgcagaag ugguaacaac cuuugaugcc caccggcccg gauucguccc ucgaaugaga     3000

cucgagaacc auaaugcuag gaagaucaug ucccugaaga aaggcgacau gauugccuac     3060

aacgaccccg ugagcgguca acgcaucaua gcaauuguuc ggaaguuuga ucagagaaau     3120

aaacagcuuu acuuggaccc acacaacgaa gcuggcaauc uggaucguag ggagaaggag     3180

aaaacauaua agccuuuacg gcccaugcca aacccgcuca agaaauaccg cccucgacag     3240

gugagaaucg acgaaauugg gcaagucuuc gaucccggac caugguggga gaggcaggac     3300

ggcuga                                                                3306


<210>  2752
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2752
auggagcaga ccagccugga ccucucccaa gccaugaaga aaguggcuca gccccagaag       60

ccuuacagac uuggcgucga uguuggggug gcaucuaucg gagccgcggc uguaacagac      120

acugaaauuc uggguauaca cgugaggacg uucgagaaag gcgaaaucaa cgggaaguca      180

ggacggaaac cauugaaugc cgauuaucgc cgacagaagg gccaacguaa gagauuaagg      240

cggaaaaaga accgccugcu aagucucaga aggcuguuuc ggcgcgucgg uaugauugag      300

agcgcagcca ucgaggcuuu cccgauuucc ucuaccucgc ccuggcagcu ucgaacagag      360

gggcuggaca gaagguugac uaaugaggaa ugggcacagg ugcucuacca uaucgccaaa      420

caccggggau uucagucaaa ccgucgcguu gauauggacg aggcuaccaa gcugagcgau      480

gccgcgggcg acaguccuga aacaaccacu aauacagagu ccgauucuga caccaaaaag      540

augcuggcag gcguggccga uaacaagaga cucuuagcug agaaagggua uaggacgaua      600

ggagaagccg ucgcacggga cccaaaguuc agccaucgaa uccgcaauaa agaggaaucc      660

uacucacaca cuauugguag agaugagauc aacgcugaac ugaauugccu uuuucaagcc      720

cagaaccauu auggcaaucc ccacgcgacc gaggaguuga gugaacagau uaucgaccug      780

auaaacugga ggcggaagac agugacuggg gacgauauua aacgcauggu aggaagaugu      840

acccuagagc cuaaggaaua cagggccgca auccacagcg uuucugcuga ggaguuccgu      900

uggcucucca cacugucacg gcuucgaauu gugacccagg gcgaaagcag accacugacg      960

aaaugcgagu gggacaaggu cguggauuug cccuggaggc aagaagccau cacuuauaca     1020

aguauccgca augcuauugg ucuccggcau gacauaagau cuagguuuaa cauccgcuac     1080

gagaccgggg gaaagaaaac uaagacagau accaaaaaug aaacgaccaa gggccagucc     1140

gccgacgcag ugacaagcga ggucaaaacu gccgaaaagg agaccgcuuu caaggcgaaa     1200

ggcuaccaca uucugcgaaa ggcauauaaa gccuuugggu acgaugagca gugggaccgg     1260

gaucguuuca acacagaauu acuggacacu cucgcuaccg ccguuacauu uuauaaggau     1320

gacucggaga uccaagcaca ucuugaaacc aacgacacuc cugaguacgc cauacagauu     1380

gcucugagau ugccaacgcu aacaaaauuc ucuaagcugu ccaauaaggc cauccucaaa     1440

auccugccgc acaugaggga gcgccaguca uauaguaacg caugucuucu ggcgggauac     1500

aagaaucacc auaaacagua ugaugacacu ccccaaaacg guaagauucc ucggagcgcu     1560

aucgaacaca uuaaaaauac cgugguauac agauccuuga accaggccag gcgagugaua     1620

aaugcaaugg ucaaggagua cggcgcccca caggaagugc auaucgagcu cgcucgcgau     1680

auggaaaagu cugccaaaaa gaaagacgag auugaucugu ggaugaagaa acggaagaaa     1740

aagaacgacg aguuaagaga aagguuuggg aagaaucuga gccgugauga gcuacucaaa     1800

uauaagcugu acgaagaaca gaacggacaa ugcgcauauu cacuucagcc cauccaguug     1860

ggccgccugc ucgaaccuaa uuacguugag guggaccacg cucugccacg gaguagaucc     1920

auggauaaca gcuauaauaa caaaguccuu gugcugacca agcagaauag ggacaaaggu     1980

gaucgaacac ccuucgagua cuucggggaa gaccaagagu cuguagauug gaagcgguuu     2040

accaacuggg uugaaucaaa cccgcagaug ccucaggcca agcgcagaaa auugcucacu     2100

aagagucugc agaaaggcau cuuagacaau uucagcgcga ggaaccuguc cgacacacgg     2160

uauauugccc gagagcuaaa gacgcucaua gaacauuaca uccugaauac ccccgagaga     2220

gagaaaacua aguacgaacg cugugugguc auuucuggaa aacuuacagc ucaaaugagg     2280

caguuguggg gcaucucgaa ggcacgugau gugagcgacc ggcaccacgg guccgaugcc     2340

gcugucauug ccugcaucac cccaucaaug gugagaagga uaacacgcua ucugcagcuc     2400

caagagagga gagcaaugcc uagugcgccc ggaaagggug acgaacaucc agccguugug     2460

gcucgggcca ucccccacgc accuguacca uggccgacuu uugagcauga ucuggaggcu     2520

gccuuagaaa aaguccuggu gucuaggccc cagagccgca agugguccgg cccuauucac     2580

gcagacacca ucuacgggga gcacaaagau caggaaacga acaccguugc cccaacacag     2640

cguauuaaac uuacucaauu gaccaaguca aauuauaaga acaucguggg auacgacgag     2700

gaaagaaaua aggauuucau agaccagauu gaggagcggc ucgaauguca uaacuuuaau     2760

ggcaaacagg cuuucgguac aagccagucu cccaucuaua ggauuaagaa aaacggggag     2820

cugggcccug aagucaagaa agugacuauc aagcuaccaa uaaagaacuc cguauuucgc     2880

auccgaagag gauacgugaa aaaugcgaag aauaguacca uuuauaucaa cauuuaccaa     2940

aauacgaaaa caggcaaguu cgagaugcac cccguuuacg ccaaagaucu gaccagcucu     3000

gcaaacgcuu ccaagucauu ugaauauagu ccggaccucc agcuggucga uacuauccgg     3060

cgaggggacu acauagagau uacacgcaga aacggagagg ugaucagcgg uuauuacgcc     3120

acccauaaua ucucgacuuc cgugcuugaa uugcagccuc acgacaccac agauucuagg     3180

aacaaucgga cgccagccau uaagaccagc cugaacaucg agcguuucca uauugacaua     3240

uuaggccgca aacgaagagu cguuaggcgg gcuaagaaug ggcucgcaga ugaccgcgau     3300

aaauga                                                                3306


<210>  2753
<211>  3309
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2753
augggcuaca gaaucgggcu ggacauugga auaaccagcg uggguuaugc cauccucaag       60

acagaugaga aaggcucccc cuacaagauu gaacaccuua cuucugucau cuucccuauu      120

gcugagaacc caaaagacgg gaagucacug gcaaugccga ggcgggaaca gcgcggagcc      180

cgacguagaa auaggcggac gaaauuucgc aagcauagaa ccaagauguu guucguuagg      240

agugaguuac ugacaaucaa acaaauagaa gcgaucuaua gcgagaagca gaaacuaucc      300

ucuauuuacg agcuccggac uaaggcucug aacgaacgcc uuaccaauga ggaacuguuu      360

cgaaucuugu auuucuuuuc gggccacaga gguuucaaau caaacaggaa aagcgagcuc      420

acagagaagg auauggggcc cgugcugacc gccauuaaag acaucgaaga gaagcuggca      480

gauggaacuu accggacacu cggcgaauau auggaguuag acgaaaaaua ccaggagcau      540

aagcguaaua aagauggcau agaccgcuac cuggggaccg ccaagagaag ucuuauugag      600

gaugaaauca aacagauuau caucgcucaa cagcaguuug gaaacacgaa guugacuucc      660

gguuuccagg uagcauuuau uggcgacgag gaacaaaccg ggauauucaa ugaucagagg      720

gaguuugaag acggaccugg caagcagucu ccauaugccg guaaccagau cgagaaaaug      780

auuggggcuu gcacauucga ggaaaaugag aagcgggccc ccaaagcgag cuacacuuuu      840

caauauuucg auuuccugug uaagcuaaac aaucucaaau accgagaaac caucggaggc      900

gaguaucgcu uucugucccu ugaacagaag aacgugcuga aggagaaagu cuugacaacc      960

cagaagauaa cguucucagg cgugaaaaag cuccuggacu uaccugagca cgcacaauuu     1020

aaucugguua acuacgggac uaaaaguaca gugaaggaaa ccgagacuaa gagcgucuuc     1080

uaugccauga uuucuuacca uaaacucaag aaagcuaucc cagcccccau cuuuuccaau     1140

cuuucaacag cagaacugga cgagauugga agaaucuuga cccuauacag cagugauaag     1200

gguaggaaaa acgaguucua uucucggcug gcuauuccug aagagguggu agaagcccuc     1260

cugccaauaa acuuuuccaa guucggcaau cuuagccuga aggcgaugcg caaaaucauu     1320

ccguacuugg agauggggcu cguuuauucg gaagccgcaa cgcaggcugg auacgacuuu     1380

agacacaaga aaaucgauca ggccuauauc cacgagaacg ugaccaaucc cgucgugaag     1440

agggcugccu cucagacaau uaaaauagug aacaauauca uucguaagua cggcgagccu     1500

gacgguauca acauugaacu ggcacgggag uuauccaaaa auuucucaga acgaaagaag     1560

acugucaaau aucaagagga aaacagagcc aggaaugagc gcaucgcuga uaagcugcgg     1620

gagugcgaac uaccaguuaa cggggcgaau auaaccagac ucaaacuguu ugaggaacag     1680

ggcgaguucg accccuacac aggacuuaag aucccuuuug auagggcauu cagugaagag     1740

uacgaggugg accauauuau cccauauagc aaaacuuugg augacuccua cggcaacaag     1800

auucugguau cugccaaggc uaaucaggaa aaagggaacc gcauccccau ggaguaucuc     1860

cagggaaagc cggaacgaau aaagaagcug gagagcauua ccgccacaau caaaaauccu     1920

aagaagcggg agaaacuucu gaagcaacag uugaccaaag aagaugugga cgguuggaag     1980

acucguaacc ucaacgacac gagauacauu ucaagucugu uacacgauua uuucaaucag     2040

aacaucuccu uugcaaaacu ggagagcgaa aagaagaaaa gggucauagc cgugaauggc     2100

gcuaucacau cuaagcuacg cgcccggugg ggguucaaca aaauuagaga gaauggagac     2160

gaacaucacg cacucgaugc gcugaucguu gcuuguguga ccgacaagua cauuaaagag     2220

aucacuucau uuaguaagag gaaagagguc cgauucaacg aaaaucuuug gaagcgcgag     2280

aagacacccc aggaaauaga gcaagaagug guacacuuua aaaaggauua ugaccggauu     2340

uucaaccagg ccuuuccauu gccuuggacc aauuucagag aggagguuag cugcaggaug     2400

uccgaaaacc ccgcagagcu gaugaaaaau cauaccuggg aaacuuacac agaugacgag     2460

aucgccgcuc uccguccaau cuuuauugug cgcaugccca acaugaagac gucuggcccu     2520

gcccaccagg aaaccauccg gucggguaaa cuggaggaga agggguuaaa uauuagcaga     2580

gucuccauag aaaagcugaa acuugauaag gagggcacua ucaaaucagg aaacgcacag     2640

uucuacaagg acaguggcaa uggguggcaa uugcuccaug auaaacugaa gguggcucua     2700

gaaaagcacg acggagaugg uucuaaagcc uuuccagacg gcgucuucaa guaucaguac     2760

cuggacaaag agcauacagu gaggaagguu cgacucgugg cgaaaagcac ccugcagaca     2820

ucccuuaacg agaagaaguc aguagccgau aacgggagca ugauucggau cgacgucuuu     2880

cgcaaugaac aaggaaaaua ucaguucgug ccgauuuacg cuaaggauag aguugccaaa     2940

aaguugccca accaggcagu ggcugucggc aaaaucgcca aggaguggcc ucugauagaa     3000

gagucugacu ucaaguuuuc cuuauauagu aaugaucuca ucagggugaa acacaagaaa     3060

gguguaaagc acgugagcaa agacgggaac aaggaagaga aaucuaagac ugauuucuuu     3120

ggcuacuaua ccggagcaga cauugcgacg gccuccaucu caggcauuag uccaaauaac     3180

uucuuugagu uccggagcau ggggcuggcu acccuuaucu cgauagaaaa guacaaaguu     3240

gauuacuuug gaaauuauca ucaggucaac gagaaggugc gacaaaaauu ccccaagaau     3300

agaauguga                                                             3309


<210>  2754
<211>  3312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2754
augaaguacg acaucggcau ugauauaggg guggccagcg ucggagaggc uguuaucgac       60

caggaaggua acauucugga ggcaugcucc aaucucuucg augaagccga cgcggcuucu      120

aacguggaua gaaggaauuu ucgggagggc cgccgaaaca aacguagaga aaggacccgg      180

guaaaugacu ucaagaaacu uuggacaaag uuuggguucg agauccccaa aaacgugaug      240

aaugauacua uucuguugcg caacaaggga aucaagugug aguuagaccu gacggaacua      300

uauucagucc uccuguacau gcuuaaacac agaggcauaa guuaucugga ggaugccauc      360

gacgaagcaa aggguagcaa uuacgccaaa gggauugcuu ugaaccaaaa ggagcucaaa      420

gagaagcugc cuugcgaaau ccagcuggag aggcucaaga uuuauggauc cuaccggggc      480

gauuguaucg ugaaaaagga agacgaggau gaauaucauu cuaauguuuu uaccauaucg      540

gcauacaaaa aggaguuaga gauucuguuc cgcaaccaga aacuuccaga agaguuuauc      600

cagggcuaca ugaagauuuu cgaacgaaaa agagaguauu acaucgggcc gggaaaugaa      660

aagucaagga cagacuaugg uguguacacu accaacaagg acgaggaggg ccacuauauc      720

acagauaaaa auauuuuuga aaaguugaua gggaaaugca gcgucuacaa ggacgaguuc      780

cgggccgcug gagccaguua uaccgcgcaa gaauuuaacg ugcugaacga ucuaaauaac      840

cucacuguaa auaaccguaa acugacagag aaugaaaaga agaaaaucgu ugagaagauu      900

aaauccucua acgcagugaa uaugcgcagc aucauaaaga aaguccuugg cgaggacauu      960

gaacaguucg guggggccag aaaggauaag gcugacaaag agaucuacca uaccuuugaa     1020

guguacagga agaugaaaaa ggaguucgag aaaaacggaa ucgauauugc cgacuucucc     1080

cgggaaaagc uggaugagau cggcaauauu uugacgcuca acacugaaac cgagggcaua     1140

aagggggcac ugcacgaagc uaaaaauuca uuuacauaua gugagaucga gacuuuaauu     1200

gacauccgaa agaaaaacag cucucuguuc uccaaguggc agucacucag ccuuaaaauc     1260

augaaugaac ugauucccca gauguacgcc caaccuaagg agcagaugca guugcuaacc     1320

gaaaugggag uguuuaaauu caagaguguc aaguuuaaaa acucuaagua uauaccaguu     1380

gaggaaauca uuaaagauau cuacaauccc gugguagugc gcuccgucag aacaaccauu     1440

aagaugauca acgagacgau aaggaaauau gguuacccug acaacaucau uaucgagaug     1500

ccacgggaca agaauagcac ugaagagaag gaacgcauua aaaaggagca gucgaaaaac     1560

gaaaaggagu augagggcau ccugaaaaga auaaaggaug aauacgacau ugagaucaau     1620

agggaugacu accguaagca agaaaaacuc gcgcugaaac uuaaacugug gaacgagcag     1680

gaggggcggu gucccuauuc uggaaagaug auuuccaucu acggcuugcu cgaagauccu     1740

cagcuguucg agauagacca uaucauucca aaaucaauca guuuugauga cagccgaaau     1800

aacaaggugu uaguuuauag auccgaaaau caaaagaaag guaacaauac accguacaug     1860

uaucugucua agggggauaa aaacuggaau uacgaccagu auaagagcua cgugcuaggc     1920

cucaaaaaca agggaaauau uucagauaaa aaggucaaca auaugcuguu cauggaggac     1980

aucaacaaga uagaugugcu uaaaggcuuu auuaccagga acuugaauga cacucgcuac     2040

gccagucggg uaguucugaa caugcuccag uccuucuuua augaaaagga cgggugcccc     2100

acaaaaguga aggucaucag agggagcuuc acccaccaga ugagggugaa ccugcgcauc     2160

aauaaagauc gagacgaguc uuauucacac caugcagucg augcuaugcu uauuguguac     2220

agucaacggg guuaugaggc cuaccguaag auccaggagg cuuguguuga cuuugaaacg     2280

ggcgaaauua uagaugagga aagauggcag cagugcaucu gggggaagga cuaugaucaa     2340

gugauguacg aggccaaaug gagcaagauu agggagaaaa ucauugaagc agagaagaac     2400

guaaaauauc accaucgcgu cgacaagaag gugaaucggg gacuguguaa ccagaccauc     2460

uacggcacaa gaauaaaaga ugguaagauc uacaaaauuu ccucuuauaa uaucuacgac     2520

aacaaggaau gcgccucguu gcucaaaaug auuaagggag gcaaggagag caaaauccug     2580

auguauaauu acgauuccaa gacuuauagg gacaugauaa aaauuaucga ugaauacuca     2640

gacgagaaga acccuuucgu ugcuuauaug aaagagaccg gagacuacuu ccgaaaguac     2700

gcgaagaaac acaauggccc aaagaucgaa aaagugaagu auuacaaaga ggaagcaggg     2760

aguuguauug auauaucuca caaguaugga uacgagaaag guagcaagaa ggucaucuua     2820

ggcucccuga accccuaucg cacagacgug uuuuucaaug agcggcuaaa gaaguacuau     2880

cucauuggga ucaaauacaa ccauauuaag ugcgaaggaa acaauuacgu aaucgaugag     2940

gacgccuaua acggccugcu uagagaugcu ggugugauau cacacgaaaa uacuaucaac     3000

gacuuggaga aaguuggggu caaguaccug uuuagccucu auaagaauga uauuaucaaa     3060

uacgaaaaga acggcgagau guucguggag agguuucugu cucguaccau ggaauaugcc     3120

aauaacuaca uugagacaaa accuauccau aagccauucu uucagaaaaa ggacaagaaa     3180

ggaaauauaa agaacuccca gaauuuagug ggccugagua aaaccaagua uauugggaaa     3240

cuugucacug augcauuggg aaacacguac augguugaaa aggagaaguu cagccucauc     3300

guggacaucu ga                                                         3312


<210>  2755
<211>  3315
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2755
augaagcuga uccucgcccu ugacauuggc auagcuagcg uggggugggc aguccuggau       60

aaaaaguccg agaccguuuu ggaagccgga ucuaacaucu uccccgaggc gucagcugcc      120

gguaaucagg ugagaaggga aaugcggcaa gcacgccgaa uuaaacguag acagaggaca      180

cgguuagacg auuuuaacaa gcuguggcgc aaaaauaacc uaaguauccc ucaguacaag      240

agcacugaga uuguaaaucu caaggugaaa gcccugagag aagagaucuc ccuugacgag      300

cuguauuuga uacucuacuc uuaucugaag cacaggggca ucucguaccu ggaagaugcu      360

caggacgauu cacucagcag uuccaacgca uauacgaaug gguuacggga gaacgccaaa      420

gcucuggaaa agcauuaccc augcgagauu caacgcgacc gacuucagac caucggaaaa      480

uauagaggcc agacacagau uacugaugac aauaacgaga agaucgauuu gucuaauguc      540

uucaccauag gugccuacag gaaggaaauu aaaaaggugc uggagacaca aaaacuauac      600

cacaacgaac ucaccgacga gcugugugau gacuauaucc uuauuuuuaa ucggaagcgu      660

aaauacuaug aagggccggg aaacgaaaag agccgcacug acuacggcag auucacaacc      720

aggcuggauc agaauggcaa cuauaucgac gaggaaaaca ucuuugagaa auugauuggg      780

aagugcuccg uuuaccccga ugaacuccgg ggagcggcag ccucauauac ggcucaggag      840

uucaaucugu uaaacgaccu gaauaaccuc acuauaaaug gucgaaaacu ugaagagagu      900

gagaagcaug ccaucgugca gcgcauuaag accagcaaca cagucaauau gagaaaaauc      960

auagcagauu guaugggcga aacuauugag aacuuuucug gggcuaggau cgacaagucc     1020

ggaaaagaac aauuccacaa guuugagacc uacaauaaga ugcggaaauc acugagcgaa     1080

aucggcacag auauugacga guucagucgc gaugaguugg acaagaucgg uuacauuaug     1140

accauaaaca cggauaaaga agcccuaaug gaggcguuua augaaucugg gcugaaccuc     1200

gacgacuccg ugaaggagug ccugaucagc uucagaaaag ccaauucguc uuccuuuuca     1260

aaguggcaga guuucagccu uaagauuaug aacgagcuga ucccugaaau guauauccag     1320

ccaaaagagc aaaugacuuu gcucacagaa augggaguag caaagggcuc cucuggcgau     1380

uucgcugggc ugaauuacau ucccguugac gugauaagcg augagaucua uaacccuguc     1440

gugaggcguu cagugcggau uaguuuuaaa aucuuaaacg ccguccugaa gaaauacaag     1500

guucuagaca ccauugugau cgaaaugcca cgagauagaa auuccgacga ggagaggaag     1560

cgcauaacug auagccagaa acucaacgaa aaggagaugg aguucaucga gggaaaacug     1620

gcucagauuu auaauaucaa acuuucuuca agugacuuua gcucccagaa gcaauugucu     1680

cugaagcuca aacuguggaa cgaacaggau gguauuuguc uuuacucggg ccggacaauc     1740

gaccccgagg auauaauuaa gaauccugac cuguucgaga ucgaucauau uaucccaaga     1800

agcauauccu uugacgacuc aaggaguaac aagguauugg uguaucgcuc ugaaaaucag     1860

aaaaagggga accagacccc guacuucuac aucacgcaca gcgauggagc cuggaccuau     1920

gagcaauuua aagcaaaugu caugaaccuc uccaagaaaa aggaauacgc cauuucacga     1980

aagaaagugc agaaucuguu auauagcgag gacaucacaa agcgggaugu ucugaaaggc     2040

uucauuaacc guaauaucaa cgacacuggu uacgcuucua gagugguccu aaauaccgug     2100

cagaacuuuu ucaaggcgaa agaagcagau acaaagguaa agguuauaaa aggguccuau     2160

acucaccaga ugaggaguaa caugaagcuc gacaaaaauc gcgaugagag cuacucucau     2220

cacgccgugg acgcuaugcu gaugggcuuu uccgagaugg gauaugaagc cuaccggaga     2280

cuucaaggcg aguucauuga uuuugaaacc ggggagaucu ugaagaacga cauguggaau     2340

gagaaaaugu cagaugacgu cuacguggau uaucuguacg gaaggaagug gagugcaauc     2400

cgaaacgaaa uugucaaggc cgagaaagac gugaaguucu ggcauuucau cguucgcaaa     2460

agcaaucagg gucucugcaa ccagacaauu cggggcacca gagaauauga cgggaagaaa     2520

uacaagauaa auaaauuuga uaucaggacu aaggagggag ugaagcuguu caaaaaguua     2580

gcuuuuucgg aaaaagaguc cgaccgugag aagcugcuug uauauuugaa cgaucgccag     2640

acguucaaua accucgucaa aauuuuugaa gacuacaagg augcccccaa uccuuucgug     2700

caauaugaga aggaaacagg cgacguugug cggaaauacu cuaagagaca caacggucca     2760

aggaucgaua aacugaagua caaagacggg gaggucggcg cauguauuga uaucagccac     2820

aaguauggac uagaaaagga cucacgaaaa gugauacugg agagucucgu acccuaccgg     2880

auggaugugu auuacaagga guccgacaaa auguauuacc ugguuggcgu caagcagucu     2940

gacaucaaaa gccagaaugg ggcgaacgug auugaugaag accuuuauac ccgcaucuug     3000

auuaaugaga agaugaucga accuggacaa uccagagaug accuggagca guuagguuac     3060

gaauuuaggu ucucauuuua caagaacgau auaauugagu augagaaaga aggcaaggug     3120

uucgucgagc gguuuguuag ccgaacuaug ccaaaaguga gaaacuacau cgaaaccaag     3180

cccaucaaua aagcuaacuu cgagaagcag aauccggugg ggcucggaaa gacaaaaagu     3240

auuaagaaau aucgcaccga cauacugggc aacagguacc uuugcgagaa ggaaaaauuu     3300

acuaaguucu guuga                                                      3315


<210>  2756
<211>  3315
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2756
augaaccacu acgugcuggg ccucgacauc gggauuacca gcgucggaua ugguguuaua       60

gaucuugaca auuaccaguu cguggauuau ggcguaagac uguuuaagga ggggacagcc      120

gaagacaacg ugaauaggcg gacuaaacgc ggaucccgac guuugaagag aaggcggcaa      180

acgcgcuuag augacaugaa agagcugcua cagaaggaga aaaucauguc ugaaaccuac      240

cauucaaguu augaucccua cgacaugaga auuaagggcc ucagccagaa gcuguccaac      300

gaugaacuua caugcgcuau ccugcacauu acuaaaugua gggguaccac auuggaggca      360

cucgacucug ccgaugagga cucggaaggg accaagucaa uccugagcaa aaaugaucag      420

gaccugaaga aauaucauua cgucugccuc gugcaaaagg agcgguuaaa gcagagugga      480

aaaauacgcg gcacugaaaa caauuucaag acagacgauu acaucaaaga gcuggacgag      540

auucuuuccc accaggauuu gucugaagac cugugugaga agauccgaac cauuguuagc      600

agaaggcggc aguauuacga aggcccuggg uccgagaaau caccaacgcc guauggacgu      660

uggaucgcgc aagguguggc ucccauagau cuaauugaaa agaugcgcgg caaaugcagu      720

gucuuuccug accagcucag agccgcaaag aacagcuaca cugccgagcu guucaaucuu      780

cugaacgauu ugaauaaccu cgagugugaa ggggagaagc ugaccacauc ucagaaacag      840

gacgugaucg auuuuguaaa ugaaaaggga ggcauuacuc caaaacaauu agcuaagcug      900

cucaacguug accuuaauca gaucaaaggu uucaggaucg auaagaacga caagccccug      960

uugaccgagu uuaaagggua uaaggcauuc aaaaagauuu uugacacaaa caauugcgau     1020

cuauacaaaa aggaccuguc cauacucgau ggacuggccg aaaucgugac cggcaagaaa     1080

ggcauugagg agcggaagca ggcuauccaa caggccuauc cugaaauaga cgaggcgauu     1140

gucgaacagu gugcagugau gaaagggaug acgcaguacc auucacuuag ccugaaggcc     1200

augcacuuga ucaaccaaga gaugcuccag aguccacgaa aucagaugca agugcugcac     1260

gaaucucaug ucuucgauaa aaaccgcguu ucccacaagg gacagaagua caucgaggcu     1320

gacgaugagg ccauuuuaag ccccguggua aaaagagcac agagggaaac uuuuaaggug     1380

gucaaugcuc ugcgggagaa auauggugag uucgaaucga ucgugguuga gacaacccgc     1440

gacagaaacu cucaggaaca gaagaaaagg cuaaaugauu cccaaaagua cuaugagcag     1500

gaaaaccgua aaguggacga gcuccuguca caugaaggcu acgauccuag uaagauuaau     1560

gggaagacua aacuuaaggu ccgguuguau cugcagcagg agggaaaaac agccuacacc     1620

aacgcgccaa uagaccucaa ucugcuuauc aaggaugcca cggcauauga gguggaccac     1680

auuaucccgc ugagcaucuc cuuggacgau ucuauuaaca auaaagcucu cauaaaccga     1740

accgaaaauc aaaacaagag caacaugaca cccaucuacg ccauucugag aggcaaguua     1800

aauaucucac uggacacuua caaaaccgua guuaagaaca auaggaacua uacagguaaa     1860

aagcgcaguu accuacucua ugauaaagac auuacuaagu uuuccaauau gcaggaguuc     1920

aucgcucgga accuggugga uaccagcuac gccugcagag ucgugauguc uacacuuuca     1980

gacuauuuca aggauaauga aauaaguacc cacguccaua cugugagggg gcgcguuacg     2040

aacauguuuc gaaaucggau caacaugcag aaagagcgug aaaaggacua cuugcaccau     2100

gcaguggaug cccugauugu agcuagcauc aaaaagcuca aucuguuaaa cacauaucug     2160

cuuaaauaca auuucaacga cuuguacaau gaggagaccg gcgaagucuu ugcgguggga     2220

gaugaccaac agguucucga uccuaaguau auuagauuca ucacucagcu gaagaccaua     2280

uacgacgagu ccaacaugua uuacaacggc cuaauugaaa ggaaugugau gcaguuucca     2340

cccaucaaaa uuucucacaa gaucgacaca aaaccuaacc gccaaauauc ggaugagacc     2400

aucuauagca cucggacagu cgggaaugaa gaguacgugg uaaagaaaua uuccgacauu     2460

uacgauccaa aguucacgca gcugaccgac gauaucauua acgacagacc ccucgauagg     2520

uggcugaugu accgaaauga caaccagacu uuuaaaauca uacagucaau uauccuugau     2580

cauuuccaca ucuuuaagga cgaugagaag cacuauaguu guaccaaaaa gaaaggacaa     2640

auucaguacg cauugaaggg uaaaaauccg cuguauuuau acaagcagga acauggcaag     2700

gugcgcaaau augccaagaa agggaacgga ccugagauca caaugaugaa guacuucgac     2760

ggcaaacucg guaauaacau ugacauaucu aaucacuauc auguucacaa caagaagguc     2820

gugcugcuuc agaucagccc cuaccggaca gauuuuuacg uguccccaga agggaaauau     2880

aagaugcuga cugucagaua cgcuaauguu uucuauaaag agucaaagaa aaaguacgug     2940

auugacgaga agugguaugc cagcgagaaa aagaggaaaa acaucaccga ugacgauauu     3000

uuugugugcu cuuugcacca ugacgaacuc aucggcauac aaaagaaaga uggagcaaag     3060

uacguauaug accuguccac ggaagauggc gggaagccuc gucuauacca cggagagaug     3120

gaaauccuga aauucaccgc cacaaauaac gacaguaaga acauuaucga ggucaaaccc     3180

auuuacacuu auuguaagaa acagcucaug aucagcauag guccaauuac caagcuggcu     3240

aaauacgcca cagaugugcu uggcaauguu uaugagguga agaagaacau caucgaccau     3300

uugucucacc uguga                                                      3315


<210>  2757
<211>  3318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2757
augggcauca gaauugggcu ggacauagga aucgccagcg uggguugggc ugucguugau       60

gacgauuaca acauuaucga guccggcgca aauauuuuca acgaagccaa ggcggcuucu      120

aauguggaca ggcggaccuu ucgccagaca aaacgacucc uucguagaca aaagacuagg      180

cugcgggauu ucgacaucuu guggauaaac gccggguuag auauccccaa agagaaggua      240

aauaaacagc ugcaguuucg cgugcaggga augacgaugg aacuaucaga gaacgaacuc      300

uaugcagccc ugaagaauau gcuuaagcac agaggcauua guuaccugga ggacgcuuug      360

gaugaggaac aaagcgguaa auccaccuau gagaaggggc uccaggucaa ccagaaagca      420

cugaagugcg acaaaaugcc uugugaaauc caguacgaga ggcugaugaa guauggaucu      480

uaccggggcc auauugaggu gaaggaugaa ucgggcgagg aaaugauccu cucaaauaua      540

uucacauuag acgccuauug caaagaggcu aagaaaauuc uggaauguca aaagcaguac      600

cacagcuuua ucacugaggc cuucgaggau gacuaccuug cguugcugcg ccgaaaaaga      660

aaguauuacg aagggccagg aaacgagcag aguaggaccg acuaugguaa auacacaacc      720

aagcuaaauc cggaaacugg cgaguauguu acagaagaga acauuuuuga gaagcucauc      780

gggaaaugcu ccguguaccc caaggaaaug cgggcaucug ccgcuagcua uaccgcccag      840

aucuacaaua ugcugaacga ucuuaauaac cugacgauua acggacguaa auuggaguac      900

gaagagaaga aauccauagu cgaagaggug caaaaggcau caaguguaaa uaugcucaag      960

aucauuaaaa agguuaucaa cgaggacaua acuaccauug aaggcgcucg caaagauaag     1020

aaugacaaag agaucuucca uaagauggaa acauauaaca agaugagaca cuacuuugag     1080

aaaaaugguc uggaguucaa gaaaaucagc acugaugacu uagaugaaau ugggaacauc     1140

cugacccuca auacagagaa ggaagccauu cuugaggcgu uuaacaaauc ucagcuggaa     1200

uugucccagg aggagaagga agccuucaua gagaucagga agaaaaaugg aucacuauuu     1260

aacaaguggc auagcuucag ucugaaaauu augaaggaac ucaucccuga gcuguaucuu     1320

caaucuaaaa aucagaugca gcuguugacc gacaugggcg uguucaagaa aaagacggaa     1380

aucuuucaga acuacgauaa gauuccaacu gacgucauaa ucgaggauau uuauaauccc     1440

guggugaaac gguccauccg aauuacaguc aagaucguua acgcacucau caaaaaguac     1500

ggcuauccuc gcgagguggu aauagaaaug ccaagagaca ggaauagcga cgaggagaaa     1560

aagcggauuc aagaugaaca gaagaaaaac gacaccgaac ugaagaaaau cauugagaag     1620

guggagucgg aauacgggau caaaauaacu gauacacacu uccgcaacca caagaauuua     1680

agaaugaagc ugaggcuaug gcaugagcag gacggacguu gucucuacuc ugguaaaguc     1740

auugauaucu ccgaacugcu uaacgacugc ucaaaguuug agauugauca cgugaucccc     1800

aaaaguauca gcuucgacga uucccggucu aauaagguuu ugguguauag caccgagaac     1860

cagaaaaaga cgaaucaaac cccuuaccag uaucuggaag gcaacgggaa ggagugguca     1920

uuugacgaau acaugaauca ggucauagag cucaaagauc gaaagcugau uaguaaagcu     1980

aagaugaaca aacuucuguu cagagaagac aucacaaaga uugagguguu gaaaggauuu     2040

aucgccagga auauaaacga uacucgcuau gcuucccggg uaguucucaa uagcuuccag     2100

aaguacuuug agucuaagca aucagacacc aaagugaagg ucauuagagg caguuucaca     2160

caucagauga ggaaaaagcu gcaguuaagc aaaaaccgcg acgaauccua ugcccaccac     2220

gcaguggaug ccaugcugau cuguuacucu cagauggguu acgaggcuua ucgacaacua     2280

caggaagacc ucaucgaucc agacucggag aagauucggg aucuggggcu uuggaaggaa     2340

acuaucaccg aggcgaaaua cgacgagguc uuguauacag caaaguggcu ggaaaaucgu     2400

ggcaaaauuc aggaggccga aaagaaagug aaguuuuggc auaagguuga uagcaaagcu     2460

aacagagccc ucugcaauca aaccauaagg ggaacucgcg ugaucgacgg cuccauucag     2520

aagaucaaca aaauuaacau cuacucagua gauggguaua agacguucaa aaauaugaua     2580

cagaagggaa aggagaaaga cuuucugaug uaccggaacg auagaaggag uugggacgau     2640

uuaaugaagg ucuaucgaga auacucugac gccaaaaaug cauucgcugc cuaccaggag     2700

gagacaggug acuaugugcg caaguacgcg aaaaagcaca acggcagcaa gaucaccaau     2760

cugaaauauu gugcaaagga acuugggucc ugcauugaua ucucacauaa auacggauug     2820

gagaaggaca gccggaaagu uauucucgaa ucucuguccc cguauagaag ugauguguac     2880

uauaacgcug gccuaaagca auacuacauc gucgguauua aaaauagcga ccuguguuuc     2940

cucaaggggg aguauuuuau agauucuaag agguaccugc agauccuucg ugaggaaggc     3000

guguugacug agcagcacac ccugaacgac uuagccgcaa ucggaacaga guuccaguuu     3060

ucccucuaug aaaaugauua ccuggaauau gagaaaaacg gcgagauuaa gaccgaacgc     3120

uuccuuucac ggacuaaacc caagcagaaa aauuacauag agacaaagag uaucgacgcc     3180

ccuaagauua aaaacaagaa acaaaaucug guaggguugg cuaagagcaa aucgaucaag     3240

aagauuagag uggauauccu cggaaacagg uauuacgccu ccaaagaaaa guuuucucug     3300

augguugaca aauucuga                                                   3318


<210>  2758
<211>  3318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2758
auggacaugg ccuggagacu gggccucgau cuugggacca acagccuggg augggcugca       60

uuguccuuag acgccgcggg ugcucccgau gccauccugg cagccggcuc uaggauuuuc      120

ggggacggac gggauccuca gucaggcaca agucuagcug uggaccgccg agcagcccgu      180

gcugccagaa ggcggcgcga uagauuuaag caaaggcagc gggcgcuccu gaaacaccuu      240

gaggcagacg gucuguuccc agccgauccg gaaguccagc aggcuuuggc cgcacucgac      300

cccuacgcuc ugcgcgcccg agcgcuggau gaggcccuca gcuuacauga aauagggaga      360

gcacuguuuc accuuaauca aaggcgggga uuccagucca accguaaggc ugaccgcggc      420

aaagaugagg acgccggcaa gaucgcuauu gggguugaca gauugaaaga ugccaucgca      480

gccgcuggag cgaggacuuu uggugaguuc cugcggcagc gacgcgcagg cgccacgggg      540

gagaaucaga uuccuucugu gagaaccagg cuacgggcug agacaggaga aggcgccaag      600

gguucggggu augacuuuua cccaucacgc gcacuccuga aggaugaguu cgacgccauc      660

uggcaugcuc aagccgaaca ccaucccaaa guacuuacug augaggcaua ucacagacug      720

cacgagauag uguuuaggca gcguccuuug cgggcgccaa aggucggagc uugcacccuc      780

gugcccggcg aagcccgacu gccuaaagca cauccauuau uccagagaag gcgccugcuc      840

gaggaacuua acgcccugau gaucguucgg gcuggcgccg uggcagagag auugacaccg      900

gaacagaggg accuacugcu ccugaaacuu aaagauaagg ggaaggucac cuuuaaaagc      960

cugcgcgaga agguguugaa acucgacgga gaugcucgau ucaauaagga gagugaacac     1020

cggacugagc ugaaagguga cgaaguagcc gcggagaugg gcgggaagac acguuuugga     1080

uccuuauggc cccaucuguc uaccgaacaa caguggacgg uuauugagag acagcaagcc     1140

cuagagagcg augcugacga agcagccuuc aggacuuggc ucguggagac ccacaaacug     1200

acagcugaac aggcccgcgc agucgcgggc gcccggcuuc cugcugguca cgggagauuc     1260

ggcuugacug ccaccgcagc ucugcucgcc gcacugaggg acggacgaac accagagggc     1320

cgcgggccca acgccccugc uaccgaagcg ggacgggugg uggucuacuc cgaggccgca     1380

gcuaucgccg guuaucauca cucagaucau agaaguggcg agguuuuuac ggacgccaag     1440

gggaggccag cccuucccua cuauggagug ccgcuggauc gucacauugu accuggcacu     1500

gcagaccccg augaaccaga cgaggccgcg cgcaucgguc gguugacaaa uccuaccgug     1560

cauauagcuc ucaaccagcu gagaaggguc gugaaucgau uaauucgggu uuacgggccc     1620

ccagccgaag uggcacugga gcuagcccgc gagcucaagc ugagcgaaga ugagaaaaag     1680

gaaagaaaca gggaaaauuc ucggaaccga cuugacgcug aaaagagauc cucaaaguug     1740

gcagagcugg gccagcgcga uaauggaggc aacagggccc uccugaaauu augggaggaa     1800

cugaaucccg agaacauccu ugaccgucgg uguauuuaca gcgggagaca aaucaguauc     1860

ggaaugauuu ucucuggugc ggcugaaguc gaucacauau ugccuuuuga cgccacucuc     1920

gaugacucca acgcaaauaa gauccugugc cuaagggagg ccaaccgcga caagcgaaaa     1980

agaagcccau ucgaggcuug gggcgggaca ccgcaguggg aagagauugc cgaacgggca     2040

ucgaggcugc cccgcaauaa gcguuggaga uuugagccug augcuaugga acgguucgcc     2100

gaggaaggag gcuuucucgc gaggcaccug guggacaccc aguaucuugg ucgcaucgcc     2160

caugauuacu ugcgaugucu guauccagac aaaggggaug gaucuuccca cguauggguu     2220

ucacccggca gauuaacgga gauggugcgg aggaagcucg gccugaacgg gcuucuggga     2280

gaccauaauu ugggcgcaga gcagccuaaa aaccgcaagg aucacagaca ccaugcuaua     2340

gacgccgucg ugaccgcuau ucucgauagg aguaugcugc aacggaucca gcgugcaagc     2400

ggugaagggg ccuccgacgc cgagcuacug cgcaucauug ucccagaacc cuggacagga     2460

uuccgagaug accuccagag agcuguggau aggaucguug uggcgcaccg ggcagaccau     2520

ggcacuguag ccaaggcugc cacccgcggu agagaccaga cagcagccag gcugcacaau     2580

gauacugcuu acggcuuuac cggggaggcg gacgccaaag gaacaccgau ugucgugcau     2640

cgggcaccuc uuggcgcuuu gaagaaacca gagcacauag aucaaguucg agacccccug     2700

uuaagagccg cacuccacga uuucaccgcc gggcugucug gaaagccuuu ugaagaccgc     2760

aucgcugccu uccccaggcu uggcccacug gauuaucggg guauucguag agugaggguc     2820

guggagccuc uacagguaau ccccauccgc gacgcggcag ggcgggccua caaaggauau     2880

aagggcgaua gcaacuaccg auacgacgug ugggaacucc cagaugguaa gugggagcag     2940

agaguucugc agauguauua cgcucaucaa ucaggcgaac cgccuaggcc ccacccagcc     3000

gcucgcaaag ucuugagucu gcaucgggac gacguguuag caauugagag aggggaggga     3060

ggccgugaac ucgugagggu cguuaaguuu uccacuaaug aguucacgcu ggcuccccuu     3120

aacgaagggg gagcucugaa aagccggcac gccgauaagg gcgacccuuu ccgcuauuug     3180

uacccaucuc ccucaacacu caaagcaugg cgagcgagag aggugagggu ggaugaacug     3240

ggucggguac uagacccugg guuuccggcc cgcaagagaa ggcgagucac ccggccagga     3300

cgugcugaug ccgacuga                                                   3318


<210>  2759
<211>  3321
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2759
augcaguaca ucguggccau ugacauaggc gucgcuagcg uuggguaugc agugaucgau       60

aagaccuccg agacaguaau ugaagccgga ucuaacaucu uccccgaggc gucagcuacu      120

gacaaucaag aaagaagggg uaugcggcag gcccgccgac ugaaacguag agagaggacg      180

cggcucgaug acuuuaccaa acuuugggaa uaccacggcc uggcaauucc ucaggugaaa      240

aagaacgaua ucgucggguu gaaagugaag gccuuauaug agaguauaag ccuggacgag      300

guuuaccuaa uccucuauuc cuaccugaag caucgcggaa uuucuuaucu ugaagaugug      360

gaggacucgu cagucagcgg caguacagcu uacgcaaaug gucugaaauu gaacgccaag      420

gaacucgaga aaaauuuccc augcgagauc cagaaguaca gacuggaaaa cauugggaaa      480

uauaggggac aaauccagau agaggaugac cagggcgaaa agcuggaucu cuccaaugug      540

uuuacuauug gcgcuuacac caaggagauc cagcaaauuu uagacacaca gaaaaaguau      600

cacgccgaaa ucgaggauga guucugugaa aaauaccuga agaucuuuac ccggaaacgc      660

aaguauuacg aggggccggg aaacgaaaaa ucucgaacug acuaugguaa guacacaacc      720

agacuuaagg agaauggcga auacauuacg gagaaaaaca uauucgagaa guugaucggg      780

aaaugcagcg uauauaagga cgaacugagg gcggcauccg ccucauacac ugcucaggag      840

uuuaaucuac ucaacgaucu gaauaaccuu accguuaaug gacggaaacu ggaagagaac      900

gaaaagcauu ugauugugga caaggucaaa acaaguaaca cuaucaauau gcguaagaua      960

auugccgaug caaugggcga gaaaaucgag gaguucuaug gugcucgcau cgacaagagc     1020

gaaaaagaaa uuuuucacaa guucgaggug uacaacaaga ugagaaaagc ccucgcggcc     1080

auccauguga auauugagga auuuucuagg gaugaccugg augagauagg guauaucuua     1140

accauuaaca cagacaagga agcaaugaug gaggcuuucg auaauucccg gcuggaacuc     1200

cccggagaga ucaaaaacug ucuuaucuca cugcgaaaga ccaauggcac guuguacagc     1260

aaauggcaca guuuuucucu aaagauuaug aacgagcuga uaccugaaau guaucgccag     1320

ccaaaggagc aaaugacucu ccugacagaa augggcguca ugaaagggca gaccgacgag     1380

uucgaaaaga auaaauacau ccccguugau guggccuccg aggacauuuu caacccugua     1440

gugagaagga gcguccggau cucguuuaag auucuuaaug cucugaugaa aaaguacgga     1500

acuuuggacg aggugguuau cgaaaugcca cgcgauagaa acucugacga gcagaaaaag     1560

ugggaaaagg agacacaaaa gaagaaugaa aaagagcagg aguauauaga aaacaagcuc     1620

gccaccgugu acgauaucag gcuguccuca gcagacuuca guagccagaa acaguuaaau     1680

cugaagcuaa agcucuggaa cgagcaagau gguaaaugcc uguauuccgg caagauuauc     1740

gaacccgagg accuuauuaa acacucugag uuguuugaaa ucgaucauau aauuccucgu     1800

agcaucucau ucgacgauag ucgguccaac aagguccugg uguaccgaac ggagaaucag     1860

aacaaaggga aucagacccc auauuacuau cucacacaca cuaacagcaa guggucuuuu     1920

gaacaguaca aggcccuggu aauggaccuu aguaaaaaga aagaguacgg acugucaaag     1980

aaaaagguua gaaauuugcu cuauagcgaa gauauuacca aggcugacgu gcugaggggc     2040

uucaucaacc gcaauauaaa cgauacaucc uacgcggcac gggucguguu aaauacuguc     2100

caagguuauu uuagagccaa agagaugggg accaagguga aaguuaucag gggcucuuac     2160

acacaucaga ugcgcgugaa caugaagcug gacaaagacc gagagaaguc guaugcucac     2220

cacgccguag augcaaugcu aauugccuuc agccagcucg gauacgaggc uuaucggaaa     2280

cugcagggcg aauuuaucga cuucgaaacc ggggagauuc uuaauuccga aaaguggaag     2340

gaucaaaugu cagacgaggu cuacaaacag uacuuguauc guaagaaaug gaguaacauc     2400

agaaaugaga uaacuagggc cgaaaagaac gugaaauacu gguauuacgu ugaucauaag     2460

ucuaaucgcg gacuguguaa ccagaagauu cgggguacga gaguguauga cggcaaaauc     2520

uacaagauca acaaacucga uauuaggaag aaagaggggg cagcgcuguu uaagaaguua     2580

gcuuucagcg aaaaagaguc cgaccgagaa aagcugcuug ucuauaaaaa ugauaagaaa     2640

acauacgagg acuuggugcg caucaugcag gauuacucag acagcaagaa cccguuugua     2700

caauaugaga aggaaaccgg agauuacgug cggaaauaug ccaagaaaca caauggcccc     2760

agaauugaca agcucaaaua cauagacggu gagguugggu cuugcaucga uauuucccau     2820

aaguauggcu acgaaaaggg aaguaggaaa gucgugcugg agagccuagu gccuuaucgu     2880

auggacgucu acuacaagaa agaggaacag acuuauuacc ugguuggcau caagcagucu     2940

gaugugaaau gucaaaaggg ggaguaugug auugacgaag augcauacgc caucaaccuc     3000

guaaaagaga agaugauaca gcagggacag aagcgcuccg accuggaauc acuugguuuc     3060

gaguuccaau ugaguuuuua uaaaaaugau aucauugagu acgaaaagga cggcaaaauc     3120

guccgggaga gauuccugag caggaccaug ccaaagguga aaaacuauau ugaaacaaag     3180

cccaucgauc gagcuaagua cgaguggcac gaccggaaau uaguugggcu cggaaagacc     3240

aaacgcauaa gaaaguacag gauggauauu cugggcaauu auuaccuuug caacaaagaa     3300

gaguuuucga aguauuguug a                                               3321


<210>  2760
<211>  3324
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2760
augggcuaca ccguggggcu ggacaucgga gucgccagcg uugguguggc uguacucgau       60

gagaacgaca auauugugga agcagucucc aacauauucg augaggccga cacaucuaau      120

aacaagguga gaaggacucu ucgggaaggc cgccgaacga aacguagaca gaagaccagg      180

aucgaggauu uuaaacaacu gugggaaaca ucaggguaua uuauccccca caaguugcau      240

uuaaauauua ucgagcugcg gaacaaagga cuaacugagc uccugagucu ugacgaacug      300

uacugcguuu ugcucagcau gcugaagcac cgcggcauau ccuaucugga ggaugcggac      360

gauggugaaa aggggaaugc uuacaaaaag ggacucgccu ucaacgagaa acaguuaaag      420

gagaaaaugc cuugugaaau ccagcuggag agaaugaaga aguauggcaa auaccauggc      480

gaauuuauua ucgagauuaa ugacgaaaag gaguaucagu cuaacguguu caccacaaaa      540

gcauacaaga aagagcuuga aaagaucuuu gagacccaaa ggugcaaugg gaacaaaaua      600

aauacuaagu ucauuaagaa auacauggaa aucuaugagc ggaagcgcga auacuauauu      660

ggaccaggua acgagaaauc gcgaacagau uacggcaucu auaccacgag aacugacgag      720

gaagggaauu uuaucgauga gaagaacauu uucggaaaau ugauaggcaa guguucaguc      780

uacccggaag aguauagggc cagcagugcu uccuacaccg cacaggaauu uaaccugcua      840

aaugaccuca acaaucugaa gaucaacaau gagaaacuua cagaguucca gaagaaagaa      900

auuguggaga ucauaaagga cgccucuagc guaaacaugc ggaaaauuau caagaagguu      960

aucgaugaag acauugagca guacuccggu gcucguaucg auaaaaaggg gaaagagauu     1020

uaucacacuu uugaaauaua ccgcaagcug aaaaaggagu ugaagaccau caauguggac     1080

auugauucau ucacaagaga agaacucgac aaaaccaugg auauccugac guuaaacacu     1140

gaaagggaga guaucgucaa ggccuuugac gagcaaaaau ucguguauga agagaaucug     1200

auuaagaaac ucauagaguu ccggaagaac aaucagcgac uuuuuagcgg auggcauucu     1260

uucuccuaca aagcgaugcu gcaguugauc cccgugaugu auaaggagcc uaaggaacag     1320

augcaacuac ugacagagau gaacgucuuu aaaucaaaga aagagaagua cguuaauuau     1380

aaauacauuc cagaaaacga ggugguaaag gaaaucuaca aucccguggu cguuaagagc     1440

auucgcacca cugugaaaau ccucaacgca cugauaaaga aauauggcua cccugagagu     1500

guggucaucg aaaugccaag agauaagaac ucugacgaug agaaagagaa gauugacaug     1560

aaucagaaga aaaaccagga agaguaugaa aagauccuua auaaaauuua cgacgagaag     1620

ggcaucgaga uaacaaacaa agauuauaag aagcagaaaa agcugguguu gaaacucaag     1680

cuguggaaug aacaagaggg guuaugccug uacuccggua aaaagauugc caucgaagac     1740

cuacucaacc accccgaguu cuuugaaauu gaucacauca uaccuaaaag caucucgcug     1800

gacgauucua gguccaauaa gguacuuguu uauaagaccg agaacucaau uaaagagaau     1860

gacacgccau accauuacuu gacccggauc aacggaaagu ggggcuucga ugaauauaaa     1920

gcuaaugugc uggagcuccg cagaaggggg aagauugacg auaaaaaggu caacaaucug     1980

cuuuguaugg aagacaucac aaagauagau guggucaaag gauuuauuaa ccguaaucug     2040

aacgacacuc gguacgccag ucgagugguu uugaacgaga ugcagagcuu cuuugaaucc     2100

agaaaguauu gcaauaccaa agugaaggua aucaggggcu cucucacaua ccagaugcgc     2160

caagaucugc accugaaaaa gaaccgggag gagagcuauu cacaucacgc agucgacgcu     2220

augcugaucg ccuucaguca gaaggguuac gaagcguaua gaaaaauuca gaaggacugu     2280

uacgauuuug agacugggga aauccuagac aaagagaagu ggaauaaaua cauugaugac     2340

gaugaguucg acgauauacu cuauaaggaa aggaugaacg agauccgcaa gaaaauuauc     2400

gaagccgagg aaaaggugaa auacaauuau aagauugaca aaaagugcaa ccgaggccug     2460

uguaaucaga ccaucuacgg aacacgggag aaggauggca aaauacacaa gaucuccagc     2520

uauaacauuu acgacgauaa agagugcaau ucucuuaaga aaaugaucaa cucagggaag     2580

ggaagugacu ugcugaugua uaauaacgau ccgaaaaccu accgugacau gcucaagauu     2640

cuggaaacuu acagcuccga gaagaauccc uuuguugcau auaacaaaga aacgggugac     2700

uacuucagaa aguauucuaa aaaucauaac ggcccuaagg uggagaaagu caaguacuau     2760

ucggggcaaa ucaacagcug uauagauauu ucccacaagu acggacaugc uaaaaauuca     2820

aagaaagugg uauuagugag ucugaaccca uauaggacag acguuuacua cgauaaugac     2880

accggcaagu auuaccuugu cggugugaaa uauaaccaca ucaagugcgu ggggaauaag     2940

uacgucaucg auucugaaac uuauaacgag uugcuccgca aagagggcgu ucugaauagc     3000

gacgaaaacc uagaggauuu aaauuccaag aacauuaccu acaaauucuc acucuauaag     3060

aaugacauaa uccaguacga aaaaggaggc gaguacuaua cagaacgguu ucugagcaga     3120

auuaaggagc agaagaaccu uaucgagacc aaacccauua auaagccuaa cuuccagagg     3180

aaaaauaaga aaggggaaug ggagaacacu cgaaaucaaa ucgccuuggc uaagacaaaa     3240

uacgugggaa agcuggugac ggauguauua gguaacugcu auauagucaa cauggaaaag     3300

uuuucucucg ugguugacaa auga                                            3324


<210>  2761
<211>  3324
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2761
augggcuaca cccuggggcu cgacaucgga auugccagcg uggguuucgg ccuuauagau       60

aacgucgacg ggaagauccu ggaguccgga guuagauugu uuucuucagc ugaucccgac      120

aauaaccaga caaggcggag uagccgccaa gcacgacguu uaaauagaag gaaagugcac      180

cggcuggaac gcguagccca ucuacucgag gaagcgggcu ucgagaaggg ugaagugcug      240

gcuccuacuc cauaucaccu uagagucaaa gggcugacgg agaaguuguc caaaccggag      300

guguacgccg cacucuauaa ccugaugaag cauaggggaa uuucuuaccu ggaagauguu      360

gagcucgacg aagccgauuc gucaagcgug agucagaaug ucgaguuacu gaaggaguau      420

uaccccugcg aaauccagcu ugagcgguau gaagaguacg gccagauucg cggcaucaua      480

gaaaccuaca acaaagacgg ggagaaggag uccuugguga auaucuuucc uacaggagcu      540

uauaaaaagg aagcagaggc cauuauccug aaacaagguc aguucuaccc agaacuagau      600

aagguauuuc ucgagaaguu cacugaaauu cugaccguua aacgacccua uuacgugggc      660

ccugggaaca agaaaucuag aacagacuau ggaaucuaca gggagaaugg cgagacccuu      720

gaaaaccugu uugauauauu gauugguaag uguacuaucu auccacagga ggcucgggcc      780

gcgaaauuca gcuacacagc acagcucuuu gacuuccuga augauaugaa caauauuucc      840

aucgccgggg aaaaguuaac cgagguccaa aaacgugaaa ucuaccacca gauuauguca      900

aguaagacgg ugaacaugau gaagauaauc acuaaaguga ccggaugccu gaaggaggag      960

gucacaggcu aucgcguuga cagcaaauac aaggcugaau uucauucuuu cgccuccuau     1020

agacaguuua ggaauaaaau gcucgacuac ggcuucgaug ugcagacucu uaacguagag     1080

auguucgaca agcuggcagc uaaguugacc cuaaacacag aauaucacca aauuaucacc     1140

cagcuggagu cacucaaucu ggggcuuagc aaagagcuga uagaaaagau ugccgauuuu     1200

cggaaaaaga accgaaguuu guucucuaaa uggcacuccc ucagccugaa ggcgaugaau     1260

gagguggucg acgaauuaug gucgacgucu aagaaccaga ugcaaaucuu uuccgagcug     1320

ggagguuuca aaucaaauau cgaaguguuu aagggccuaa aauacauucc ccucgaugcc     1380

augguugacg agaucuauaa cccugugguc cgcuguagua uuagagagag cauaaagauc     1440

auuaaugcac ugcuuaaaaa guacggggcu uuggauaagg ugguaaucga aaugccaagg     1500

gacuccaacg agaaagaaga gaaggaucgg aucacuaaac ugaauaagga aaacgacaau     1560

aaaaagaaac aggccauuga gaaggcucgc aacgaguacg gauucggcga agaggccuau     1620

agacagcaga augaucucaa cacaaagcug aggcuuuggu acgaacaaaa acagcguugc     1680

cuguauucug gugagauaau caagauuuuu gacuugauca aaaauccgca ucucuucgac     1740

auugaucaca ucauacccaa gagcaucuca uacgacgaua gucuguccaa caaaguuuua     1800

uguuaugcaa ccgaaaaccg ggagaagggg aaucugacuc cuuaccgaua uuuuaagaga     1860

aaagccagcc uaacauggaa cuacgaggcu uacaagucuu augugauuga acucuacaaa     1920

gacggcaagg gagaucuguc aaggaccaaa cuugaguugc uccuguucga agaggacauc     1980

aauaaguaug agguccgcca gcgguuuauu aacagaaauc ugguggauac gagguacgcg     2040

aguaaggucg uguuaaacac ccugcaggac uucaugaaag aaaauuaucc aacaacuacc     2100

guugaggugg uacgcggcaa guuuacagca caacuucgac ggcauugggg gauccguaaa     2160

aacagagaug aaaaucacgc ccaccaugcu auagacgccu ugauuaucgc augcgucccc     2220

caccucagcc uguggaagga gcagaaaaag guguucucca cugaugaguu caggguugac     2280

gaaaccacag gagagauuau cgaacagucu uacaaccagc uaaccuauca agagccuuuu     2340

ucgcaguucu uuuaccagcu ggaaaaggug agcauagauu gucgcuacuc cuauaaaguc     2400

gaccggaagu ucaauagaca gguggccgac gcuacuaucu acucaacgag gcaagguauu     2460

guaaaacaga caaguggcga uaagucuuuu acccucgaca gcaacuccaa agagcauuau     2520

gugaucgcca agauuaaaaa uaucuacgaa gauguugacg caaagcgauu cauggaucgc     2580

uauaagaaag acaagacuca guuucugaug uaccaccaug auccacaaac cuucucaaua     2640

cuugaggacg ucaugaaaac auaucccgau aagaaaaacc cguuucaggc guaccgguug     2700

gaguacgggc cuauuagaaa guauagcaag aaaggaaaug gcccccagau ccagucuauc     2760

aaguacuaug acuccaaacu gggucaacac auugaauuaa agcaggcuaa ccagaguacc     2820

aaaaaggugg ugcuccagag ccugaaccca uggaggugcg auaucuacua uaauaagaaa     2880

gacaagcguu acauugccgc agggcuuaaa uaugccgauc ugucuuacca aaagggcacu     2940

ggauuguacg gcauaucccu ccagcgcuau cagcagaucc ugaacucaga gaaaauugaa     3000

cuaaguaucg agcaaauuga acugcuccug gaggggcggc uuaaugagag caacuucgaa     3060

uuuuguuucu cguuguacaa gaaugacuuu auccagugga acgacgagaa uaaccagaca     3120

uuccaauaua gauuccuggc uaggaauuua uccucuccua accgaauaga agucaagccc     3180

aucaauaaaa gcaaguacga uaaacaggcc gagggacuca agacgcugaa aaagggucuu     3240

aaccgguuuu auaagauuuc aguugacgug cugggcuacc agcacuucau caaaaaggag     3300

aaacuaaagc ucaccuuuaa auga                                            3324


<210>  2762
<211>  3330
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2762
augagccacu acgugcuggg ccucgacauc gggauuaccu ccgucggaua ugguauaauc       60

gaucuugaca caaacgauuu cauugacuac ggcguuagac uguuuaagga agggacugcc      120

gcuaauaacg aagauaggag aaauucucgc ucacgacgua gauuguuaag uaggaaacau      180

acgcggcuga uggacaugca gaaggugcua aaagaggcag gaaucaugac cgaaagcuau      240

ucuccccucu ccaacguaua cgagauucgc aagaaaggcc ugacaaagga acuuacuaau      300

gaugagcuga ccgccgcgau cuugcacaua acaaagcaua gagguucggu gaucgagacc      360

gucgaagacg augaggcugc cgcaaaagaa acuggggagc ucaagucaac acugcacgag      420

aacgcccugc ucuuagcuaa uaacaaauuc gugugcgaaa uucaacugga gaggcuuaau      480

accgaaaaca agauccggaa ucauaaaaac aauuuuaaga cgaaggacua uguugcagag      540

uugaaagaaa uucugaacca ccagaaucua agcgaggagg ugaagaacaa aaucauacag      600

auugucgaac gcagacgagc cuacuaugag ggaccuggca guuucaacuc cccaacuccg      660

uacggcaggu uuaucgaagu gaaugggcag guacaagaga uugaucucau cgaaaagaug      720

cggggaaaau guucuguuua ccccgacgag uuccgugcuc cuaagaugag caucuccgcc      780

gaucuguuua acauucuuaa ugacuucaac aaucuguugg ugcaggguga gaaacuccag      840

gaagaggaca agaagaaccu guuaaaaaua aucaaugaua agggcucaau uacccugcgc      900

cagaucagua aaaagcucga agucaacgag gacgaaauaa aaggguauag aauugauaag      960

aagggaaaag cgcuuuuuac agaguucaag ggcuacaaaa uccugaagaa auuguuucua     1020

aauugcgguc aaagcaucuc ucuggaggac uaugaaaugc ucgaugagau uaucgaaauu     1080

gugacuaaga agaaagggau agacgagagg aaggaugcac ugaaagagcu uaagugggac     1140

cuguccgaug acaucauuaa cucauugagc gaagugaccg gaaucgucgg cuaccacagu     1200

cucucucuga aagccauccg ggaguuagau uccgaacugu auaagacaag ccuaaaucag     1260

augcagcucc ugcaugagcu uaagauguuc gacaaaaacc gaccaucgua caagggcaaa     1320

aagaauauug uugcugaccc cgaagccaua uugucuccug uggcaaaacg cgcucagaac     1380

gagaccuuua agguaaucaa ugcccugcgg aaaaaguaug gggaguucga uuccauugug     1440

gucgaaauga cgagggacaa gaacucacaa gagcagaaaa agcggaucaa cgauagucag     1500

aaagcgcucg aaagccugaa uaaggaggug gaccaacuuc ugcagcagaa aggauacgau     1560

ccacagaagg uuaaggguaa aacuaagcaa aaagugcgcu uguacaugau gcaggaaggc     1620

aaguccgccu auacacucca ggagcuggac uuacacagag ucauuaaaga ugacaaguac     1680

accgagaucg aucauauaau ccccauuucu aucagccugg acgauucaca gaguaacaag     1740

gugcuagcac uccacuccga aaaucaacag aaagggaacc ugacuccuuu cauggcuuuu     1800

cacacaggaa gguucgccga caugggcugu accuggaaua aguuuaaaga gaacguacuu     1860

agcaauaaga acuauaaaca uaagaagaaa aauaacuugc uggaugaacg ugacauuaau     1920

aaguucucug uuauccagga guuuauaaac cggaaucucg uggacacguc auacgcuaac     1980

cgagucgugc ugaauaccuu aacagauuau uucaaagaca acgaaauuga uacuaagguc     2040

cacaccguga acgguaaacu gacagaccuu uuuagaaaga ggaucaauuu gaaaaaggau     2100

cgcaacgagg acuaccucca ucacgccauu gaugcacuga ucguugccag uauaaagaaa     2160

cuacggcugc ucgacgggua ucuggcuaag uacggcuucg agacucuuua cgaugaaaug     2220

accggagaga ucaaagaaau uccagaggac aaggcguuuu ucgaugacaa auauaucaga     2280

uacauuuuga aucugaagac aaucuaugag agcuccaaca uguacuauag gggcauauua     2340

gaacgcgagg aacucauuua ccagccgauc aagaucucuc auaaaauuga uaccaagccc     2400

aaucgaaaag ugucggacga gacuaucuau agcacgcgga uuauagacaa cgaagaucac     2460

cugaucgaga aguacuccga cauuuacgau ccuaaauuua agaaucuuac agaggacauc     2520

cugaacgggg aaaagaauaa auauuugaug gagaagaacg auccacaaac cuucaauauu     2580

cucaaacaga ucauauuuaa ccacuacaau acuuauaagg acucaaaaga auacuauaag     2640

aucgagaaga aaaaggaaaa agagauuauc aagcugguag gaauuaaccc ccuaagucug     2700

uacaaagagg aaaauggucc uaucaagaag uauucuaaaa agggcaacgg gccagucaua     2760

augagcauga aauacgauuc cgagaagcuc ggaaaccaua uugacaucac ccccgauuca     2820

gccaaaaaua agaaagugau ccugaagcag auuagcccgu accguacaga cuucuauaag     2880

ugcucugaug aaaaauucag auuuguuacc gugagguaca aggacgucca cuaucagaaa     2940

uccauagaca aguacuguau cgauaaagag ugguaucaaa acgaaaagaa gaauaaaaag     3000

auugacgaga aagcagaguu ccuuuuuagu augcaucacg augaauugau cggcauuacu     3060

cgcaaggaga gcgaacaguu caucuacgac gagucuaaag agaaccacgg ugauccucgg     3120

uaucaugacg gggugaagcc cgaaauacug aaguuuacag cuacgaauaa cgauaccaaa     3180

ggcaucauug agguaaagcc auuauacacu uacuccgccu auagacucaa accuacagug     3240

cugaauguua agaacaucaa aaaguacgca accgacgucc uuggaaaucu guaugaagug     3300

aagcagaacg agcuaaaacu cgaguucuga                                      3330


<210>  2763
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2763
auggccgagg gcaagaaaag aaucuggagg cugggggcug accucggagc aaacagccuu       60

gguugggccg cgauugaacu guccgcuccc gugaauggca agccuauagc caucuuggca      120

gccgggucuc ggauuuucuc agauggacgc gacccaaaaa guggcgcuag cuuagcaguc      180

gaucgacgug acgccagagc uaugaggcgg cgcagagaua gguuuaagca gcggcaaaaa      240

gcccugauca aguaccuaau ugcggacggu cucuucccgg cagaugaggc cgcucgcaac      300

gccauggcag cuaucgaccc cuaugccguu cgagcgagag cccuggauga agcacuuucc      360

cugccugagu uggggagggc ucucuuucac cugaaucagc ggcguggauu caagucuaac      420

cgcaaagccg acagaaaggc ugaugacgau gccggcaaaa uagcaaucgg cauugacagg      480

acccgggacg ccaucgcuga agaugaggcg gacgcagggg ccgagccagg aucguggaca      540

uuugguaagu ggcugcauga acgacgccuc aaaggccagu cagugagaac uagguuacgg      600

cccgagagcg gggaagaugc uaagggagac ggcuacgauu ucuauccuga ccgcgcccug      660

auggaggagg aauuugaugc aauuugggcc gcucaggccc cacaccaucc cgacguacuu      720

acgaaugaug ugcacgacag auugcaugag aucauauucu accaaaggcc ucugaaggca      780

ccacaggucg guaugugcag ucuaauuccg ggggaucguc ggcuccccaa agcgcacccu      840

cuguuucagc gaagaaggcu ucuggaagag uugaacgcuc ucaugaucgu gcgcccagga      900

gaaguugccc agcggcugac cagagaggag agggacauuu uacugcucaa acuuaaagac      960

aagaauaaag uguccuucgc aacacugcgc aaggccuuga aacuagaucc cgacgcucga     1020

uuuaacaagg aaucugagga ucggacugac cugaagggcg augaagucgc cgcagugaug     1080

ggcagcaaaa cccguuucgg gaaucucugg guacaccugu ccaucgaggc ucaaugggac     1140

guucuugcca gacuggaugc gguggaauca gacgccgagg augcugcauu uugugccugg     1200

uuggucgaga cauaugcucu caccccugaa caggccaggg caguggcgaa cgccccacug     1260

cccccgggau acggucgcuu cggcgagacu gcuacacgga aguuaaucga agcccugaga     1320

gacgggaaaa aggaugacag ggugcuaguc uauagugagg caguuaccga agcuggacuc     1380

ggccaucaca gcgaucgacg cacgggugag guguucgagg acgccaaagg gcggccucag     1440

cugcccuacu acggcguagc acuugaaaga cauauuaugc caggaacugg cgacccuucu     1500

gaucccgagg aaaugagggu ggggcguuug accaauccaa caguccacau aggacugaac     1560

cagcuccgcc ggauccugaa ugugcuuauu agagccuaug gucccccugc ugagaucgcg     1620

auagagcugg ccagggaauu gaagcucacu gacgaggaua agaaacgaau uaaccgggaa     1680

aauaacgaca accgcaucgc agcugagaga agguccgaaa agcuguuagc caucggccaa     1740

gccgauucag gggcuaaucg ggcacugcua aaacucuggg aggagcugaa cccagccaau     1800

guucuugacc gaagaugcau uuacagcgga cgccagauca guauugauau guuguuuucu     1860

gcggcugugg aagucgacca cauacugccg uucgccgcaa cccucgauga cuccaacggc     1920

aauaagaucg ugugucugag ggaggccaac cgugaaaaac ggaagagaag cccccaugau     1980

gcuugggguc acucggcaag gugggacgag auugccgcgc gcgcugccca guuaccucga     2040

aauaagagau ggcgguuuga accagaugca auggacaaau ucagggacga agggggcuuu     2100

caagcccgcc aucugguaga uacacaguau cuugcucguu ugucuagaga guaccucgcc     2160

acccuguauc ccgagaaggg agagggcucc gggcacaucu ggguuucacc uggacggcua     2220

acggaaaugg ugaggcgcaa acugggucuc aacgaccugc uuccagauca uaauuucggc     2280

gggggagcag accaggcuaa gaaccgauug gaucacagac accaugccau cgacgcgguc     2340

guggucgcca uuguggaucg gggcaugcug aaugcaauag cuagggccag uggucaggag     2400

ggggcugaag gccgcgagag aaucauuauc cccgagccgu gggacggauu uagggaugca     2460

uuacgggaaa cuguuaaccg uauuacagug agccaccgca ccgaccaugg cacugccucc     2520

aaagccgggc ucccuaaggg aaaggaugcu acagcgggcc gacugcacaa ugacaccgca     2580

uacgguuuca cgggggacac cgaugccaaa ggaaacucua ucguagucag aagggugcca     2640

cuuagcucau uuaagaaacu ggcugacuug gaucggauac gcgacgccga ucucaaggca     2700

gcccuguauc aacaccuaaa uggccuggag gguaaggcuu ucgaaaaggc gcucgccgag     2760

uuucauaaac uggaguggca caacuucggc gggguuagaa gggugcgggu cacagaaccc     2820

cuuacuguga ucccuauuaa ggaccgaaac ggaagaaucu acaagggcua caaaggggau     2880

aguaauuauc gcuacgacgu augggaguug cccgauggaa agugggugac caacuggaaa     2940

aaugaagcag gcgaggcuau guccagcguu auuucuaugu uugacgccca ucagucaagu     3000

gaaccaccua ggccccaccc agcagcccgg aagauccuga gcuuacauca gaacgaucuc     3060

cuggcuauag agcgugaggg ugaagacaga aaacuuauga gggucgugaa guucgggcag     3120

uccggacaaa uuacacuggc cgagcaccag caggcgggcg aucuaaagaa acgcgacgca     3180

gugucuaaug acgaugaccc guucaaguau uuugccccua cuucgggugg ccucaaaaag     3240

gucaaagcuc ggcagguucg aaucgaugaa auugggagag ugcuggaccc cggauuccca     3300

gccaggaccg cucgccggaa gacaagaggc uga                                  3333


<210>  2764
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2764
augaccgcca gaacacacaa gaucuggagg cugggcgcug accucggggc aaacagccuu       60

ggaugggccg cgauugagcu guccgcuccc guggauggua aaccuauagc caucuugcau      120

gcaggcucuc ggauuuucuc agacgggcgc gauccaaaga guggagccag cuuagcuguc      180

gaccgacgug augcaagagc caugaggcgg cgcagagaca ggcugcagca acggcagcgc      240

gcucuaauca aauaccucau ugccgauggc cuguuuccgg cggacgaaaa ugagcgaaag      300

gcaauggcca cuaucgaucc cuaugcuguu agagccaggg cacuugacga agcucugacg      360

uugccugagg ugggucgggc ccucuuccac cugaaccagc gucgcggguu uaaauccaau      420

agaaaggcgg auaggaaggc cgacgaagau gcaggaaaaa uagcuaucgg cauugaccgg      480

acccgagacg ccauccgcca ggcugaggag gaagccggcg cagagccagg gucuuggaca      540

uacggaaagu ggcuguauga aagaagggcc ucggcuacug augcgaacgc aauucccuca      600

guacggaccc gccucagacc ugagacagac gagcaugcca aaggugaugg cuacgacuuc      660

uauccaggga gggcuuuaau ggaagccgag uuugcagcca ucugggaagc ucaagccgca      720

caccaucccg augcgcugac caaugaggug cacgaccguc uucacgaaau aauuuucuac      780

cagcggccuu ugaaggcucc acagaucgga gucugcacuc ugauucgagg cgaucagaga      840

cuaccgaaag cccauccccu cuuucaaaag aggcgccugc uugaggagcu gaacgcauug      900

acaaucgugc ggccugguga aauggcccag agacucacca gggaggaacg cgacacgcug      960

uucuuaaagc ugaaagauaa ggcuaaaguu agcuuuguga gucuccgaaa gacucuuaaa     1020

cuggacccag augcccgguu caauaaggag uccgaaaacc guucugacuu gaaaggggac     1080

gaggucgcag cugccauggg agcgaagacc agauuuggca auaggugggu gcaccuaagc     1140

aucgaggaac agugggaugu aauugcccgc cuggaggaag uugaguccga cgcugaagau     1200

gcagccuguc gggcuuggcu cgccgcaaca uucgaccuga cucccgagca gauaagagcg     1260

gugaccaacg ccccucuucc acccggcuau ucaagguuug ggaaggucgc uacacaacga     1320

cugaucgaug ccuugcgcga cggagagaaa gauggucggg ugcucgugua cagugaagca     1380

gucgcugccg caggccuggg gcaucacagc gacuuaagaa ccggagagau uuucgaagau     1440

gcccagggca ggccgcagcu gccuuacuau gguguugcuc uagagcguca caucaugccc     1500

gggacgggca auccagacca uucugaugag augcgcauag gacggcucac uaacccuaca     1560

gugcacauug gccugaauca acuuagaagg aucguaaaca ccuugauccg agcguacggg     1620

cccccagccg aaauugcaau cgagcuggcu cgggaacuca agcugacuga cgaugacaaa     1680

aagcgcauua auaaagagaa caacgaaaau agacuugccg ccgagaagag guccgagaag     1740

cuguugacaa uaggacggcc cgacaccggu gcuaaccgag cacuccugaa auuaugggag     1800

gaacugaauc cugccaacgu gcuagauaga cgcugcaucu auacgggcac ccagauuuca     1860

gucgacaugc uguucagcgc ggcuguggaa guugaucaua uccucccauu uacagccacu     1920

cuugacgaua guaaugggaa caagaucgug uguaugaggg aggcaaaucg uaccaaacgg     1980

aagcagucuc cguacgaagc cuggggacac acagacagau gggaggagau ugcugcaagg     2040

gccgcgcagu ugccccgcaa caaacgaugg agauucgaac cugaugcuau ggagaaguuu     2100

aaggacgaau ccggcuucca agcccggcau cuggucgaua cucaguaucu cgcaaggcug     2160

agccgcgagu acuuagaaac ccuguaucca gguucggggg aggggucuuc ccacauaugg     2220

gugucacccg gacgucuuac agagauggua agacggaaau ugggccucaa ugaccugcua     2280

ccugaucaca acuuuggggg aggugccgac caggcuaaga auaggcugga ucaucgccac     2340

caugccaucg acgcaguugu ggucgcuauu guggaccgaa guaugcucac cgaaaucagc     2400

agagucuccg gccuggaggg ggccgaagga cgggagagga uuaucauacc acccccgugg     2460

gaugaguucc gcaacgaccu uagaacugcg gugaaugcca ucacgguuuc ucacaggaca     2520

gaucacggca ccauuagcaa agcagguuug ccuaagggga aagacgcuac ugccggccgg     2580

cugcauaacg auaccgcuua cggauuuaca ggcgaaaccg acgcaaaggg caauucaauc     2640

gugguacguc gcgucccauu agccaguuuc aagaaacucg ccgaucugga ccgaauuaga     2700

gauacugacc uuaaggcugc gcuguaccag cacuuggagg gacucgaagg caaagcauuu     2760

gagaaggccg ugauggauuu ccccaaccug gaauggcauc cuuuugguaa cguuaggcgg     2820

gugcgcguca cagaggugcu auccguaauc cccauaagcg accaaaaugg gagaauuuau     2880

aaaggauaca agggcgacuc uaacuauagg uacgaugugu gggagcugcc agguggcaaa     2940

ugggaagcug agguugucuc aacguucgac gcccaccagc cugauugggu gaguaagauc     3000

cggagccgau cccccaccgc aagaaaggug cucucucugc agcagaauga ccuuauugcc     3060

gucgaacgcg cucaugagac uagggaauug augcggguug ugaaauuucg ucaaaacggg     3120

gagaucacau ucgcgccacc gcaggaggcc ggaucgcuga agagaaggga ugcaaccccu     3180

aaugaagacg aucccuucaa auauuuugcu guggccgcaa gcuccuuaca gaaggccaaa     3240

gcucgccagg uacggauaga cgagcucggc cgaguccugg auccaggguu ccccgccaga     3300

acagcgaggc gcaagacucg gaagucaacc uga                                  3333


<210>  2765
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2765
augagaaucc ugggcuucga cauugggaua accagcaucg gaugggccua cguggagggu       60

gaagaacuca aggauugcgg cgucaggauu uuuacaaaag cugaaaaccc ccagaauggg      120

gacucccuug cagccccucg gcgcgaggcg cgaggaguuc guagaaggcu ggcucggcgc      180

aaggugagau ugaacguaau caaaagguua cuguguaagg aguucggccu aaaucucucu      240

gauuauuuug ccaacgacgg ugagcugcca aaagcauacg agacuacgaa ggauaccaag      300

ucaccguaug aacuucggag ucuggccuug gagcaaaaac ucgaacccaa ggagcuggcu      360

cgcgugauuc ugcacaucgc aaaacaucga ggguacggaa auaagcacgc caaaagagac      420

cucgaggcug aagagaaggc caagaaagcg gcagaaaagg ccccucagga gaaagaaagc      480

acaacuacca acggcgauuc caagaaagag caggagaagg ucuuaaaagc ucuguaucag      540

aaugaaacac uuuugaagaa guaccaaacc aacuaucaga cuaauaacaa aacaaaugac      600

gagaccaaca auaagacgau auuccuggug ggcaaauacc uauacaagga auuucaggau      660

aaagggcaga ggauccggaa cacuaccaau aacuaucaac auacaaugcg ucaggagugg      720

cucaaggacg aacuggaguu cauuuuuaag aaacagaagg auuucggagc cacuuuuucu      780

gagaacuucg aaucgcagau ccuugagacc gcauuuuacc aacgcgaccu gaaaucauuc      840

gaagauaagg uugguaaaug cguguuuuau gagaaugaaa acagagcucc aaaggacagc      900

uugagugcca uggaguucgu cgcgcucaca aggauuauca auacccugaa gaacuuagag      960

aaaaagucca aaaaucuggg cauaggggaa acguacggaa aggacaaaau ucaggagauc     1020

cucaagauug ugcuugauaa gggcgaaaug ucuuauaaag agcugcggaa gaucuugaaa     1080

cuaaacgaga gcauccuguu cgccaaggac uccaaacucg auuacacuaa ggguaugcag     1140

gaagcagaga aagcuaaguu uauugaacug aagaaucuua aagccuucaa gaaagcuaug     1200

ggaggggacu uuucaaaguu cccccgagag gaacuggaua aaauagccac agacaucgua     1260

uugauuaaga guaaggagaa ccucgcaaag aagcugcaaa auuauagcgc cuuaucuaaa     1320

gagcagguug aagcucuguc caaccuaaau uuugcgaagc acaucgaucu cucacugaaa     1380

gcacuugaca agauauugcc uuucaugcgc gagggccuga gcuacgauga aguggucaag     1440

aaagugggcc uccaggagca uagaaaguac aaacagaagg gggaauuucu gauuccacuu     1500

aaagaguaug agcccuaccu ggccaacccu guggucgcua gggccuugag ugaauaucgg     1560

aagguucuca augcacuguu acgcaaguac ggaaacgugc acaaaaucca ccuggaguuc     1620

accagagaag ccgguaagaa uuaugacgag aggaaaaaga ucgaaaaaga gcaacgugag     1680

cauuuugaag cuaaccagaa ggccauuaaa cagcgggaga uccuaggccu cccaauuaac     1740

ggggcaaaua uacugaagau gaaacuuugg aucgaacagg gagaguguug cguauacucu     1800

ggcgagaaaa uuacuuccaa gcacuucuuu gauagcaacg cguugcaagu ggaccauauc     1860

uauccguacu cgcgaucuuu cgacgauucc uacaugaaua aaguccuggu guuugcuaag     1920

gaaaaccaga auaaacucaa caagacaccc uucgaggccu ucgguucaga caaggaaaaa     1980

ugggauagaa uccugaguuu aagcgcaaag cugccuaaac agaagcagag gcgcauuucc     2040

aauaaagacu uuaaggauaa ggagcuuggg uucauagccc ggaacaucaa ugacaccggc     2100

uauauugcua gauuggccuc ucaauggacg aacaauuguc ucaaauuuga agaucugagg     2160

gagaacgagg uuaccaucgc aggagaaaag ggcagcaaaa aucaccuaga ggugauuuca     2220

gggcagcuga caaguaugcu ccgccacuac uggggacugg gugacaagga ucgaaacaau     2280

caucuucacc augcugucga cgccgugauc auagcguaua cuaacgccaa aaccaucaag     2340

gcuuucgcag auuuuaagaa aaaccaggaa cagaaucggg ccaaguucua cgcuaaacaa     2400

auuuccgagc aggaauauaa gcgucagaga aacccauuug agcccugcgc caauuucagg     2460

caacgcguag aggagaaaau caacaagauu uuuguuagca agccuccacg gaaaagagca     2520

aggggcgcgu ugcacaagga aacauucggg ucuaucuguc uggacggaaa uaacaauugg     2580

guggcccgau uuaaaauaaa gaguaaaaag gaacaggagg auauuacuaa agaaaaggcu     2640

accaaggcca aauacaagga cgaguucaaa ggcgaggaaa acauccgcaa ggcauuaaaa     2700

cucgguaaga uucggagaau caauaacaca cagaaggaca uagcuaucgg ggauaaaauu     2760

gucaagaaug agaccauggu gagggucgac aucuuuaaag auaagaaagg caaguucuau     2820

gccgugccca uuuacacuau ggacuucgca cugggaaucc uuccgaacaa ggccguugcu     2880

acgggcgaag augaggacgg ggugauaaaa gauuggauug agauggacaa gacaaaauac     2940

gaauuuugcu ucucacuguu uaaggaugac uugaucaaag uacguaagaa gaaaaugaag     3000

gaggcggaac ucuguuauuu cgucggauuu gauaccgacg guagccgcau cauuguggcc     3060

aaacauaaua acgauuacca gacuaugacc gagaaugaau gcaagcuguu cacacuaaaa     3120

gagaagaaaa agaagcagaa aacccugaac gacgaggaag acaaccaaga gcggaucgaa     3180

acugaguaua agauuauagg cgggagaaca ggaauccaga aucucgaaac guuugagaaa     3240

caccagguuu ccccucuggg cgaggugcaa aaggcagagu ucgaagauag gcaggaaauu     3300

aaacuuaagu cuucgcccaa ggguaaagac uga                                  3333


<210>  2766
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2766
augagcgaga aguacagacu gggccucgac guggggacca acucccuugg augggccguc       60

aucggucuga aagaagaugu ucccucuuca guguuggcua caggcguaag gauuuucagu      120

gaggggcggg acgcaaagac uaaaagcacg uuaaaggccu cccgcaccga aaaacgaucu      180

gcgcguagaa ggcgggaucg cuuucugcag agacaaacau uccuaauauc ggagauggug      240

aagucaggac ucuuuccuaa ggacaucguc gaacagaaaa aucuggagcu ucuggaucca      300

uaugagauua gguucaaggc uuugacugaa caggugccgc uccaccaucu gggccgggcc      360

cuguuucaca ucaaccagcg ccgagguuuc aaaagcagua gaaaggacaa auccgaggaa      420

accacaucug ggguugugag caaguccguc agggcacucu acgaggagau gcaauuaauu      480

aaucccgccc cugugaucga ugacccaaag gcucugucaa aagaagaacg gcgugaaaua      540

cgcguacagg aggcaaguag cagagaagag gcccuuguua ccuuggcuga gcagaaggga      600

gugacuuuug gcgccuuccu guauaggcgg cuacagucug gcaaauccgu ccgagcgcgc      660

aaggaugcag acucacaacu cuacgaugug uaucccacaa gagaacugcu uaaagacgag      720

uacagcaagc ugaugcagca ucaggcccag uauuacccua acgugaucaa ugaggcuuug      780

auuaguaggc uccacgaugu caucuuuuuc caacggaaac ugaaggagca ggaacgcggg      840

uuuugcaccu acuauccauc ugagagaagg acguuccguu guaugcccuc cuuucagcaa      900

uaccggaugg uucaggaguu aaacgcccug gaaugggugg acgcagcugg aaagcauugc      960

cuccgagacu auccugagag cagagaucuu auucugucgg aauuggagaa uaucucuacu     1020

aaagccggug aguucaccuu cggcaacaug aagaaaauac uaaagaaaau ggggguaauu     1080

gacggagaag uguccuuuaa uuucgagggc ccaaagagga aggguuuuca ggggaacguc     1140

acaucacgcg aaaugcggca cgaggauugu uucggaacua gaugggacga acugagucuc     1200

gaugagaaag acgauaucau ugacaagauc aaugaugacc accuggauga cgaggacaug     1260

gaaagguacc uuaucgauac cuauggccug agcuccuuuu cugugggcaa cgcgauuaau     1320

gcccgcuugc ucgaggggac agcaaacguu agccgaaaag cugcccggac ccuguacgaa     1380

acgauguuau cagcuaauug ccugcagagu gacgccguga uagcagucuc cgagcaagau     1440

gacacuuucg ugaacccgua uagccguaca ggaagaggug aacuacucuc ugagcugccc     1500

uacuacggcg aggccuuuca ggaugggagg cauaucauuc cuggaaagcg cgaaccaaaa     1560

guaucacacg accagcuuaa guucuauggc gguguuagua accccaccgu gcauaucgcu     1620

uugaaucaga uacggcuggu cgugaacgag auuaucaaga gauacgggcu cccuagcucc     1680

aucucuauug aacuggcgag gaaucuucca gagggccaag auggacgacg caaaaucgaa     1740

aagaugcagu cggagaacca gaaaaauaac gagcgguaug accagauucu gacugaaaau     1800

ggccaaagcc ccaacagaga uaauagguug cgucucgcac ugugggagga auuaggggag     1860

ggaccgacag accgccugug ugucuuuacc gguaagcuaa uagaacucgc cgaucuguuc     1920

aacggcuccg uggagaucga gcaccuuauu ccuuuuucag uuaguuugga cgauucuaaa     1980

gcuaauaaga cggugugcug gaaggaagcc aaccgguuca aagggaauaa gacccccuac     2040

gaggcauuug ccaacaauag cggagacuau uccugggaag agaucaugga gagaguaagg     2100

gcucugccaa acucaaaaca guggcgauuc caggaagacg cccucaagau cuggaacagc     2160

gaucacucug acuucuccgg ccggcaucug aaugauacac gcuacauuag uagauuagca     2220

agggaguauc uggaaaacau auguccuuuu aauaaaaucg acgucgugac uggucggcuu     2280

accgcguugc uccgagggca cuggggccug aacaauauuc uaagaggaca uaacgagccc     2340

gaaacagcua cuccaaagaa aaugcgcgau gaccacaggc aucacgccau cgaugcaguu     2400

gugauugcca ugaccagccu gacaauccuc cagaaggucu cuuccgcugc cggcaaggca     2460

gaggagcugg acguggggaa acuuuucgcu aaggaugaau caggacguag uguaauagac     2520

cccugggaug guuuucggga cgagguggcc gaaaaaguua gaaauaucau ugucagccac     2580

aagaccaaaa ggaagacuau cggcaacucg gggaagucca cggaucgcgc guugcauaau     2640

gagacagccu acggacugau ugacggcccu gacgaaaaag guuuauacuc uguggugacc     2700

acuaagccaa ucagcuauuu cgauaaccga gacaaaauag agaagauuag agaugagaaa     2760

cuccgggacg auuuucugac cgcuugggac ggggaaggcg auaauaaggc aucacaacuu     2820

gccguccugg acaaggcuag uggauugaac guuaaaaagu gcagguacgu ggagcgcaaa     2880

aaggugaucc cgauuaaugg ccccgauggg cagccuuaua aagccuacga cggaaacucc     2940

aauuggggca uggaaaucua ugaguuccca aacggucacc ccaaggaaaa gaaauggaac     3000

gggguaguca uaucuacaua cgaugcaaau aagagcgagu uuaaaccugg agagaccuuc     3060

cguccacauc ccgcggccaa gcucgugaug agacugcaca ucaacgacau uguugaagug     3120

cagaaugacg gcgagccgau ccuauaucgg guccaacuga uuuccgcugg uacucucaca     3180

gugucaccuc ugcacgaagc caacguugau agcaggaauc gcgacaaaca ugaugaguuu     3240

aaguacaaaa gucuuucugu gacgccaaug cagaagaugg gcgcaaagaa aguaaacauc     3300

ucccccaccg gguugaagaa uuacagccga uga                                  3333


<210>  2767
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2767
augggcauca uuauagggcu ggacaucgga auugccagcg uggguaucgc ugucguugau       60

aaggacaccc ucgauauuaa augcguggua uccgaccuuu ucgauucugc agacgccuca      120

aagaacaucg agagaaggaa uaaccggcag gcgcgccgac ugaaucguag aaggaaaaca      180

cggauaggcg auuuuaacac uuugugggug aguauguucg gggaauuacc cgaggaaguc      240

gacagcaaug ugcugcuacu ccgcaacgcu ggacuggaug agcaacuuac gcuggacgaa      300

aucuacugug uuuugaagua uaugcucaaa cacagaggca uuuccuaccu ggaggaugcc      360

cugaaugagg aaaacgugac cgguucuuau cagaagggga ucgcaauuaa ucagagggag      420

ucggaauaca ugcucccuug cgagauccag uuagagcggu uucgcaagua uggacaauac      480

cgaggcgaau gugaggccga aaacgagaau ggcgagaaaa uaacacuguc aaacgucuuc      540

augacuaaua gcuauagaaa ggagauugac aaauuucuua acacccaggg gaaguacgga      600

aucuugaauc agaaauucau ugaugaguac cugaagaucu uuaacaggaa gcggaaauau      660

uacgaagguc caggcaacga gaagagucgu acagacuaug ggaaauacac cacuggaaag      720

gaccuagaug gcaaauauau cacagaaaag aauauuuucg agaaacucau agguaagugc      780

uccguguaca aggaagaacu gcgcgcugca ggggccucuu auaccgcuca ggaguuuaac      840

auccuuaaug accugaacaa uauuacggua aacaauaaaa aguugacuac ccaacagaaa      900

aaggauguua ucgaaauagu gcagaacagc gacagaauua auauggagaa aaucaucucc      960

ucauguauug gagaaaagau cgagaagauu gaaggcgcca ggauagauaa aaacgagaag     1020

aauaucuacc auacauucga gcaguacaac aaaaugcgga agauuuuugc ggagaaaaau     1080

uaugacauca aggaacucac uaucgaacaa cuggaugaga uuggccgaau auuaaccauc     1140

aacacagagc gcgaagggau uaaggacgca aucauggaga gugaacugcu ccaccuuaau     1200

aacgagcaga uuaaccuguu gaucgaacua agauacaaaa auagcucucu guucaccaag     1260

uggcaguccc ucucaauaaa agccaugagg gagaucauuc cggaucugua ugaccggaac     1320

gucaaucaaa ugaacauccu ugcugagcgc ggaguguuua aggauaaaau uaaucuguuc     1380

gaaaacagcg uguacuugcc caaggagccu guccucgaag agaucuauaa uccaguugug     1440

agaaggagua uaucuaugac gauugacgcc cugaacgcau uaaucaagaa auacgguuau     1500

cccgaagaca uuguaaucga gaugccucgu gaucggaaug acgcugagca gaaggaucga     1560

auaaagaagu uccagaaaac uagagaaaac gagcugaaga auaucaaaaa gaagauuaaa     1620

gaagaguacg gcaucgaaau uacagacgag uacuuuagga agcauaaagg gcuagagcuc     1680

gaacugaaac uuugggugga gcagaacgaa aaaugcccau auuccggaaa guacaucaau     1740

guccaagaua uauuguauca ccaugagcug uucgagauug accacaucau cccccucagc     1800

auuucguacg augacucucg caacaauaag gugcugguuu auaaagaaga gaaccaggau     1860

aagggcaacc ggaccccucu uauguaccug uccacuguga auagacagua ugaccagcac     1920

gaauuuauga acuacgucuu gucaacaaaa acccucccaa ggaagaaacg cgagcuguua     1980

cugaauuacg aagauaucac gaagauugac gugcuaaagg guuucauaaa ccgaaaucuc     2040

aacgauaccc gguaugccag ucguagcgua cugaaugcga uccaauuguu uuucgggggc     2100

aaagagugug acacaaaggu uagagugauu aggggagccg ucacuaacca gcuucgcauc     2160

aaacugaauc ucccgaagga ccgggagaaa uccuacucuc aucacgcaau ugaugcugcc     2220

aucaugugcu auagccagau gggccugaug aacuuuagaa auauacagaa caaguucauc     2280

gacuuugaaa augagaccua cuuaucagau gaaagggaca guuguuucga uaacaaguau     2340

uacgacgagg cucuguuugg gcaagugcuu aaaaugcgaa agaauuugcu caaagccgaa     2400

ggaaagcccc agcgcuccgu ccagagcaaa gguguguucg augaggagga aaagaacaac     2460

ggcauuguuc gguaucauca caaaguggac aagaagccua auagagggcu gucuaacgca     2520

ucaaucuacg gaacaaggaa aauugaugac aagaucuaca aaauaguagg cuaugauauu     2580

cguaauaaca aggacgccga ggcuaucauc aauauuauca aaaaggguaa gagugauaac     2640

uuucuaaugg cgaaacacga cccagaaacu uucaagaauc ugauggagau auacgaacag     2700

uauaaagagg agaagaaccc cuuccucgca uacgaacaag agaccgggga cuauauucgc     2760

aaauacgcca agaaucauaa cggcccuauc auuaagaaaa ucaaguaugu cgauagcgaa     2820

gugggauccu gcauagacau cucugcuaau uacggcuaca aaaacgggga uaagcggguu     2880

auuauggaca cacugacccc auauagagcc gauguguacu auaauuacaa agacggaacu     2940

uauaggauca uuggugucaa guacgcagau uuuaaguauu cgaacgccaa auacgugcuu     3000

gacgaggagg cuuacgccuu gauccugaua aaggaaggcu uacucaaaau uggggauagc     3060

cuggagaaua ucuccucaag uggauaugaa uaccgacuuu cuuucuauaa gaacgacauc     3120

auugaguaca cgaaaaauga uaucguauau aaggaacggu uucugagccg cacaaugccc     3180

aaggugaaaa acuacauaga gaccaagccg auuuccgcag cguuguauga gaaaagaaac     3240

ccuaucggcc ucacuaaugc uaccaacauu uguaagaucu acacagacgu ucuggguaau     3300

gaaaccaaaa uauacaagga gaaauucaag cuaucagucg acuga                     3345


<210>  2768
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2768
augaacuaca ccgagaagga aaaacuguuc augaaguaua uccucgcccu ugacauuggc       60

auagcuagcg uggggugggc aauccuggau aaagaguccg aaacagucau ugaggccgga      120

ucuaauaucu uucccgaagc gucagcugcc gacaaccagu ugagaaggga uaugcggggu      180

gcaaagcgca auaaccgacg uuuaaaaacu agaauuaaug acuucaucaa gcugugggag      240

aacaauaacc uaaguauacc ucaauuuaag agcacggaga ucguuggccu caaagugagg      300

gccauuaccg aagagaucac acuggaugaa cuuuaccuga uuuuguauuc cuaccucaag      360

caccggggga ucucuuaucu ggaggacgcu cuggaugaca cuguaucggg aucaagcgca      420

uacgccaaug gccucaaauu aaacgcuaag gagcuggaaa cccauuaucc augcgagaua      480

cagcaggaac gccuuaauac aauugguaaa uacagagggc agagucaaau cauuaacgag      540

aauggagaag uguuggaucu guccaacguc uucaccaucg gcgccuacag gaaggagauc      600

cagcgggugu uugagauuca gaagaaauau cacccggaac uaacugacga guucugugau      660

ggcuacaugc ucauauuuaa ucgcaagcga aaauauuacg aagggcccgg aaacgagaag      720

ucuagaacag acuaugguag guucaccacg aaacuggaug cgaacggcaa uuacaucacu      780

gaagacaaca uuuuugagaa acuuaucggg aagugcagcg uuuauccuga cgagcugcgg      840

gcagccgcug ccuccuacac cgcacaggaa uacaaugugu ugaacgaucu caauaaccug      900

acaauaaaug gacguaaguu agaggaaaac gagaagcaug aaauugucga gcgcaucaaa      960

ucaaguaaua cuaucaacau gagaaagauu aucagcgacu guaugggcga gaauauugau     1020

gacuucgcug gugccaggau agauaaaucu gggaaggaaa ucuuucacaa auucgaggug     1080

uauaacaaga ugcggaaggc gcugcucgaa auuggaaucg acaucuccaa uuacucacga     1140

gaggagcuug augaaauugg cuauauaaug accaucaaca cagacaaaga ggccaugaug     1200

gaagcauuuc aaaagagcug gauugaucug agugacgaug uaaaacagug cuugaucaau     1260

augcgcaaga ccaacggcgc ucuauucaac aaauggcagu cuuucucccu gaagauuaug     1320

aaugagcuca ucccagaaau guacgcccag cccaaggagc aaaugacgcu gcuuacugag     1380

auggggguua caaaaggaac ccaggaagag uuugcugguc ugaaguauau accuguggac     1440

gucgugagcg aagacaucuu caacccagug gucagaaggu cgguucggau uucuuuuaaa     1500

aucuugaaug ccgugcucaa gaaauacaag gcacuggaua cuauuguaau cgagaugccc     1560

cgcgacagaa acuccgagga acagaagaaa aggauaaaug auucacaaaa guuaaacgag     1620

aaagaaaugg aguauauuga aaagaaacug gccgugacau acggcaucaa gcuaaguccu     1680

agcgacuucu ccucucagaa acagcucagc cugaaacuua aguuguggaa ugagcaggau     1740

gggauuuguc uguacucagg aaagaccauc gacccaaacg auauaaucaa uaacccgcaa     1800

cucuuugaaa uugaccauau cauuccccgu aguaucuccu ucgaugacgc ucggagcaau     1860

aagguccugg uguaucgauc ugagaaccag aaaaagggca aucagacgcc uuacuauuac     1920

cuuacccacu cacacaguga guggagcuuu gaacaguaua aagcgacagu uaugaaccug     1980

uccaagaaga aagaguacgc aauaucuaga aagaaaauuc aaaauuugcu cuauucggaa     2040

gauaucacua agauggacgu gcugaaaggu uucaucaaca ggaacauuaa ugauaccagc     2100

uacgccuccc gcuuaguccu gaacacaauc cagaacuuuu ucauggcuaa ugaggccgac     2160

acuaagguga agguaauuaa agggucauac acccaucaga ugcggugcaa ucuaaagcuc     2220

gacaaaaaca gagaugagag uuauucucac caugcaguug acgccaugcu gauaggcuac     2280

agcgaacuug gauaugaggc uuaccacaag uugcagggcg aauuuaucga uuucgagaca     2340

ggggaaauuc ugaggaaaga caugugggau gagaauaugu ccgacgaggu guaugccgau     2400

uaccucuaug gaaagaagug ggcaaacauc cgcaaugaag ucgugaaagc ggagaagaac     2460

gucaaauacu ggcacuacgu gaugcgaaag ucaaaucggg gucuguguaa ccaaaccauu     2520

cguggcacua gagaauauga cgggaaacag uacaagauca auaaauuaga uauaaggacg     2580

aaggagggaa ucaagguuuu ugcuaaacug gccuucagca agaaagacuc ugaucgcgaa     2640

cggcuuuugg uguaucucaa cgacagaagg acauucgaug accugugcaa gauuuacgag     2700

gacuauuccg augcagccaa uccauuugua caguacgaga aagaaaccgg cgacaucauu     2760

cgaaaguaua guaagaaaca uaacgguccc cgcaucgaua agcuaaaaua caaggacggg     2820

gaggucggcg cuuguauaga uauuagccac aaauacggau ucgaaaaggg cucuaagaaa     2880

gugauccugg agucccucgu uccuuaucgg auggacgugu acuauaagga agagaaccau     2940

ucauacuauc uggucggggu gaaacagagu gauaucaagu uugagaaagg aagaaaugua     3000

auugacgaag aggccuacgc aaggauacuu gugaacgaaa agaugaucca accaggucag     3060

agccgugcug auuuggagaa ucugggcuuc aaguuuaaau uaucguucua uaagaacgac     3120

auuaucgagu acgaaaaaga ugggaagauu uacacugagc gccucguuuc ccggaccaug     3180

cccaaacaga gaaauuauau cgaaacaaag ccgauagaca aggccaaauu ugagaagcaa     3240

aaccuggucg gacuuggcaa aaccaaguuc aucaaaaagu acagguauga cauucugggu     3300

aauaaauacu cuugcagcga agagaaguuu acuucauucu guuga                     3345


<210>  2769
<211>  3348
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2769
augagcgcca ccuacacaau cggccuggac auugggauag cuuccguggg auggugcgca       60

uucggugaga cucacauccu cgaucuuggc gucagagccu uugacaaggc ggaaacggcu      120

aaagaggggg aaucucugaa cuuggccagg cggaaugcac gcuuaaugcg acguagacug      180

uucaggcgga ccuggcgccu aaagaaacuc gccagacagc ugaagucaga gggacuuauu      240

gcugauacaa aacuguuuaa gcccgaauug ccuuucagua agagccucug gcaacugagg      300

guugacggcc uggaucggcg ccucaacgac gaggaguggg cacgagugau cuaucauuua      360

uguaaacaca gagguuuuca uuggauuucc agggccgaag cuaagcaggc cgagggggau      420

ucuaaaucgg aaggaggcaa gguaaaacag ggccuggcgg ggacugcaaa acuuauggac      480

gagaagggau accggucagc cgcugagaug guguuggccg aguucccaca ggcacaacgu      540

aauaagcagg gugaguauca gaaggcucug agccgcgaac uacucgccaa agagcuggcg      600

cuucuguuug cccagcaaag agaauacggc aacuugcaca ccccgggggc acucgagaca      660

gcuauccugg gaaauggcga uaagaaaagu gguuuauucu ggcagcagaa gcccgcccug      720

gcugggaccg accuccuuaa aaugcuggga acuugcacau uugagaaggg cgaauauagg      780

gccccuaagg cauccuucac cgccgagcgg caugucuggu ugacgcgacu aaacaaucug      840

cgcauaguga cugauggcgu uaccagacag cucacagaac aagagaggca gcugugucuu      900

ccacugcccu accggcaggc uggggacuug acuuauaaac aacucggauc ugcgcuggca      960

aaggccgguu uacuggaagc uggcggguuu aaauucaccg gacuagccua cccuacagag     1020

gcacagaagg ccgagggcaa agcuaaggau ccagaaagcg ccacccucgu gaagaucccc     1080

gcauggcacg agcugcgcca gacgcuuacu aaaggugggu ugaacgacga augggagaag     1140

cuggcgggcg acgcucucug cggacagccu gaaauuuuug auaaaaucgc cuggguccug     1200

uccguguaca aggacgauga cgagguaaga gcagagcuug ccaaacuggc uuugccaaau     1260

ccgcaagccc ucacagaagc acuguuaaac auuagguucg auaaguucca cucacugagu     1320

cuaaaggcuc uccgugccau cguuccccau auggagcagg gccugcggua ugacgaagcg     1380

ugugugcagg ccggguacca ccauagccag cuuuugaaag ucggagaggg ugagcacaag     1440

uaucugccuc cacucuacga uggccgagac ggggauggaa gaaugauauu ugcugaagac     1500

gcagauauuc ccaggaaucc uguggugcug cgcucuuuaa accaagcccg gaaagucguu     1560

aaugcuauca uuagaaggua uggcucccca ucagccgugc acaucgagau ggcacgcgac     1620

cugagccgac ccuucgacga acggaacaag aucaaaaagg aucaggacga guaccaggcg     1680

cguaaucaga gagauaggga acaauuuagu gagcuuuucg gucauccgcc uaagggguug     1740

gaguuugaaa aauggcgccu cuaucgggag cagaacggcc agugcgccua cucucagaga     1800

ccccugaccg cuuccggaaa cguagccgaa auuuuccuag acggcagcac ugagauagau     1860

cacgcacugc cauacucgag gucuuuugac gauuccaaga auaacaaagu gcucguccug     1920

acacgagaaa aucgcgacaa ggggaaccgg accccuuaug aguaccuugg aggugcugaa     1980

aauucagaaa guuggcaaag auucgugagc uuuguugagu ccaacaaagc cuauagguug     2040

gcaaagcgua cgcgccuguu acggaaagau uucggcgcca aggaaucuga ggaauuuaga     2100

gagaggaauc ucaacgacac ccgauacgug ugucgguucu uuaagaauua ugucgagcag     2160

caucugaaac uugcugaagg gagcgagaca aagcgcugcg ugguaguuuc aggacagcug     2220

acuaacuucu ugagagcgag guggggccuc cugaaagugc ggagugauuc cgaccgacac     2280

caugcccuag augcagcugu cguggccgcc uguagccacu cuauggucca aagacuguca     2340

aauuacaguc gcaccaggga acucgaacag gugcgugacg guuucguuga caucgagaca     2400

ggggagauug ugaaccccgc uaugcaccag cagcugcggg agcauuuucc agaucccugg     2460

ccuuauuuca gacacgaacu ugaggcaagg uugaagaucg acagcccaga acuguuacgc     2520

gaggagaugg cccgacucgg cuccuacucu gaagaggaac ugaaaguacu uaagccgcug     2580

uuugucucga gagcgcccca acggaggaau ggaggcgcug cccauaagga uacuauauac     2640

gggcagccug agaaacuaaa gaaaaacgga agcgugaccc agaagguugc acucacaucc     2700

cugaccuuga aagacauuga ucuguuagcc gacccacacc gcaaugcuaa gcucuauggc     2760

gcaauccgug aaagacugga ggcccacggu gggaagggag auaaagcguu ccccgcuacu     2820

aacccucuuc ggaagccaga caaagagggc aaccccacgg guccgaucgu gaggacaguc     2880

accaagguga uugauaaacu gucaggcauc ccuguacgcg ggggaauugc caagaaugac     2940

acuauguugc gaguggaugu uuuuaccaag ggcgggaaau uccaucucgu cccaguguac     3000

gugcaccaua gagucacagg acugcccaac cgggcaauaa ucgccuuuaa ggacgaagau     3060

gaguggaccc uaauugacga caguuucgau uggugcuuuu cucuguaucc uaaugaccuu     3120

cucaggauca aacugaagaa agaaagccac uccggcuacu auucaagcug uaaccgcucu     3180

acuggugcug uuaauuugug ggcccaugau agaaacguga auguggggaa ggacggauuc     3240

aucgagucca uuggcguaaa aauggcaguc aguauagaaa agcugcacgu ggauacauua     3300

gguaacaucu accccgcucc accugagaag aggcggggcc ucgccuga                  3348


<210>  2770
<211>  3351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2770
augagccaga accagaaucc caacacccag ucccaccuga auccuacaac ucagucuuca       60

acgcagagua acccacaacc gaccacacag gagagccuca agauccuugg cuucgacauu      120

gggguggccu ccauaggaug ggcuuuuguc gaagguggcg agcugaaaga uugcgggguu      180

agaaucuuca cuaaggcaga aaaucccaaa gacggaucuu cguuggccuu accuaggcgg      240

gaggcgcgcg gcgugcgacg uagacuggcu aggcggaagg gucgccuaaa cgcccucaaa      300

guacugauuu guaaggaauu ugggcuuaau agaucagauu accuggcaac cgccggagag      360

uugccaaagg cuuaugcaac aagcaaagag accaagaguc ccuaccagcu cagggccgaa      420

ucccuggagc ggaaacuguc ugcugacgag uucgcccgcg ugauccucca uauugcgaag      480

caccgaggcu auggcaacaa acaugcaaag gaaagcuccg augacgauuc agggaagguc      540

aaaaaggcca ucagugagaa ucagaaagug augagcgaaa agggauacac uacagcuggu      600

caauauuuau acggcgaguu uuaucagaaa gccagaaacu ucucugaagc agagccuaag      660

ucccccaaaa augcuagggg gacccaggaa uuuaagaacg uucggaauaa gucagagaac      720

uacgcccguu gccuggcgca gagcgagcuu caaaaagaau uggagauaau cuucgccaag      780

cagcgcgaau uuggauucca guuuagugac aaaaagcaca aaacgauuaa ugcaaacggc      840

aagcugaagg agcuagauuu cgcugacgcc gugcucgaaa ucgcuuuucu gcaaagacca      900

cuuaaaucuu ucgccgauaa ggucgguaaa uguacuuucu acgaggacaa gccuagggca      960

ccaaaagauu cccugagcgc cauugaguuu guggcuuuga cccggaucau aaacacacuc     1020

gcgaauauua cuaagcgauc gggggaagua uauucugcag acaagguucg cgagauccug     1080

uccauugugu uagacaaagg agaaaucacc uacaagaacc ugagaaaagu caucgcccuc     1140

gaugagaggc uucaguuccc gaaggacuca aaacuggauu augcuaagga agacgccgag     1200

aaggcaaaag ccauugaguu uaguaaguug aaggcuuucc ggaaggcccu aggcggcagc     1260

uuugaagggu ucauacgcgc agagcuggau uccaucgcga cagacauugc ucucaucaaa     1320

ucuaaggaug aacugaaagc caaacuugca aauuaccaga gccugaacga cgagcagaga     1380

gagaaguugu cagaacucag uuuugauaaa uucauagacc uguccuuuaa ggccuuagag     1440

gcuauucugc cccuaaugag gggagauaaa gacgacaaga ucuaucguua cgaugaagcc     1500

uggcgggagg caggucucgu ggaaguggcu aaaaagaccc aaaagggcga cagccugccu     1560

ccacuuaucg aguaugagaa agauuuggcc aaucccgucg uugcgcgagc ccuggcugaa     1620

uacagaaagg ugcucaacgc acugcuuaaa aagcauggga aaguacacaa gauucaccug     1680

gaguucacga ggcaggccgg agugucuuca aaggaacgcg gcgagaucau ugcugaacag     1740

aauaaacggu ugaaggccaa cgaggaggca agaaaaaggu gcgaagagua cggucucaua     1800

ccuaguggga agaauauccu gaaauuaaag cuguggcagg aacaaggcga guuuuguguc     1860

uauagcggaa agaaaauuac ucaguccgac cuacgcgaug aaucuacacu ccagaucgac     1920

cauaucuacc cauauucgcg aagcuacgau gacucccaga acaauaaggu gcugguuuuc     1980

accgaugcga accaaaauaa acuuaaccag acucccuaug aggccuuugg caaggacauu     2040

ucaaaaugga aucagauauu ggggcggauc gagaagcugc cgaaagcuaa gaagaaccgu     2100

auuacaaaca aaaauuucgc caacaaggaa gcaggauuca ucgcuagaaa uauuguggau     2160

accaguuaca ucgccaggcu cucugcaagc uauacggacg ccuuuauaga guucugcccu     2220

cuggcugaaa acgaggauac cacauuaggu cgcggcgaga aaggguccaa gaaacacauc     2280

ucagucguga auggaagccu gacugcgacc augcggcauu acuggggccu uaacacauug     2340

cucgacggug aaaauucuga guccaaggac agaaguaacc aucugcacca cgcccuagau     2400

gcaauuauca uugcuuacgu aaaugacagc guuaucaagg ccuuuucuga uuucaaaaag     2460

acucaggaac aaaacaaagc caggcugaua gcuaaggaga uugaauccgc agaguauaaa     2520

aucucacgac gcuuuuucag ucccagcggg uuugagaaua acgaagccuu ccggcaggcg     2580

auuaagagaa agauccucgg ccagucgcug gaguccaggg aaaaugacau gaccauaaac     2640

ucugaggcuc uuggagaaaa agugggcggg aucuuuguca gcaagccacc ucguaaacgc     2700

gcccggggag cauugcacaa ggagacauuc ucaaguauug augacaaaga ucuguuaaag     2760

accuacggug gcaaggaggg ggugaauaga gccaucaaac ucggaaaggu caggcaaauu     2820

ggcuccaaaa ucgcugacaa cgguacuaug gugcgaguug auauauuucg gcauaaggug     2880

ucugggaaau ucuauggcgu acccauuuac acgauggacu uugcacuggg aauccuucca     2940

aacaaggccg ucgugggcgg gaaggauaaa agcggaguua ucaaggacug gcugcccaug     3000

gauuccaauu augaguucug uuucucauug uuuaaagacg accucauucu ggugcagaag     3060

aaagaaauga gcaaggcgga acuaugcuac uucgcuggcu uugauacaag uggugggcag     3120

aucaaagucg agaagcacga caaccauuuc gccacccuga cugauaauca gcgccuccug     3180

uuuaacauug caaccccuaa ggaaguggcc ggaaaaucua uaggcaucca aaaucuuaag     3240

guauucgaga aauggcaagu guccccauug ggugagguua gcaaggcuga auuuaaagag     3300

agagaaccga uugcccugaa gucgacaccc aagagguucu uacagggcug a              3351


<210>  2771
<211>  3354
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2771
augaagcaca uccugggccu cgaccuuggg agcaacucca uuggaugggc cuucgugcag       60

caagauuuug agaauaaaca ggguaagaua aucgcuaccg gcucuagaau uauccccaug      120

gaccagggga uucugggaga uuucgaaagg ggcaacacag ucucacagac ugcagagcgg      180

acgaccuacc gcaguaugcg acguuugaga gaaaggcauu uacugcggcg cgagagacua      240

cacaggguuc uccaucugcu ugguuuucug ccuccacacu augacgccca aaucgauuuc      300

acaaaacggu acgggaaguu uauagacaau gcggaaccga aaaucgcuua uaacaaugga      360

aacuucauuu uuaugaauag cuucaacgag augguggagg auuuuaagaa gcaucagccc      420

caguuguucu acaaaaaguc caauggcgaa gagucucgca ucccuuauga cuggacuauu      480

uacuaucucc gaaagaaggc ccugucgcag aaaaucaccc aacaggaacu ggcauggcuc      540

auauuacacu uuaaccagaa gagaggcuac uaccagcuga gaggggagga ggaaacagag      600

aauccaaaca aggaaguagc cuuccacuca cuuaaagugg ucgaugugga ggcugaagca      660

cccaauaaga aaggagagau uugguauacc auccgguugg agaacgguug gauuuaccgu      720

cgcacuagca agaauccucu ggacgauugg aaaggcaaga caagagacuu uaucguuacc      780

acggaucuaa acgacgacgg gacuaucaaa cucgauaagg aaggaaagga gaaaaggagu      840

uuccgggccc cagacgaaaa cgauuggaau cugcuuaaga aaaagaccga gcaugacauu      900

gauuucuccg gcaaacccgu ggguacauau auauacgacc accuguugca aaacccuaau      960

cagaagauca aggggaaacu cguccgaacu auugaacgca aguauuacaa agaugagcug     1020

aaggcuaucu uagccaaaca gauagcgcug caaccagagc ucuuuaccga aacacuucug     1080

gcagacugca uuagagaguu guauaggaag aacgaaaccc agcagcagaa ucuacugucu     1140

aaggauuucc uccaucuguu uguggaggac aucaucuucu accaacggcc gcuuuguagc     1200

aaaaagucca cgauugccaa cugcacucug gaaaaacgcu cauacaucga uaaggagaca     1260

aaugagagaa aagacgcucc cauuaaggua ugugccaaga guaacccuua uuaccaggaa     1320

uuuaggguuu ugcaguggcu ccagaaucug cguauauaug agaucgaaac cgaccgggag     1380

gugacugcag aguucauuga aacaccacaa gauuacgagg cuuuauuuaa cuuccugaug     1440

agccagaaag aaaucgacaa ugagggacua cucaaguauu uucuguucgc caaaaacccc     1500

cagaucaagg auaaagcggc caagucugaa cuuaaaaagu gguugaccac guaccgaugg     1560

aauuaugucu acgacgcagc ugauaacucc ucaaagaaau acccuaugaa ugagaccggc     1620

uaugagcugc agagauaccu ccaaaaggug gaaggccugc cagccaacuu ucuuacauuc     1680

gacauuacuu aucagcugug gcacuugcuc uacagcguga aagauaaggu cgaguuugaa     1740

aaagcucuga agaaguucgc caaaaagaaa caguuagaca agaccaguuu cguugauucu     1800

uuuaaaaacu ucaagcccua uccgaaugag uacggguccu uuagcgaaaa ggcaaucaaa     1860

aagcugcuac cucucaugag guucggaaac cacugggacu uuaaucgcau agaugugucg     1920

acacagaaac ggauugacga ucugaucaac gguguagcca augaggacau uagaacuauc     1980

auaagggaga aggcugaaaa auaucaacuu aacucugaga ccugcuucca ggacuugccc     2040

cuguggcucg cgcaguacgu ggucuacaau cgccaugcag aagccacaga uaucgagaag     2100

uggaccuccg ugucagaccu ggaggcuuau uuagaggauu uuaagcaaca cagucugcga     2160

aacccaauug uugaacaggu ggucacugaa acgcuucggg ugguacguga caucuggcag     2220

caguacagcg gcgggcaagc caauuucuuu gaugagauuc auaucgaauu gggaagagag     2280

cucaaaaaga cagcagagga aaggaaacag cuguccgaca agaaccagga gaaugaaaac     2340

accaaucuac gcauuaaaaa gcugcucgcc gagcugaaag aagaugcuuc uauacagaac     2400

guucggccuu auagccuugu gcaacaggag uugcugaaga ucuacgagga aggcgucuuc     2460

ucaaguacug acgagauuga agaggauauc cagaagauaa ggaaaaaggu ggaacccacc     2520

ccacccgaga uccagaggua uaaauuaugg cucgagcaaa aguacaaauc cccuuauaca     2580

ggugaaguca uuagccugac caaacuuuuu acugaggaau acgagaucga acacauuauc     2640

ccacagucuc gauacuauga cgauucacug aguaauaagg ugauauguga gagcgccguu     2700

aacaaauuga agacaaacca gcucgggcug gaguucauuc aaaaucauca cggcgaaaaa     2760

gugcgcauca cggcaaacaa ggagguaacc aucuuuacug aggaacagua caaagaguuc     2820

gucaagaagc acuaugcgaa uaacuccgcu aaacagcaga agcuacugcu caaugaaauu     2880

ccggagaaaa ugguggcccg gcaacugaac gacacaagau acaucucuaa gugcauuucg     2940

gaaauacuua gcaaaaucgu uagguccgau gagaaggacg aaggagucaa uucaaagaac     3000

auuauccccu guaccggcaa aauuacaacu gcauugaagc aggacugggg gcugaaugau     3060

guguggaacg aguuaauccu cccucguuuc gagcgcauga aucagcugac ccagacgcaa     3120

cuuuuuacca guuauaacga ccggcugcag aaaaaucuac caacagugcc cauagaauac     3180

ucucagggau uccagaagaa aagaaucgau cauaggcacc augccaugga cgcucucgua     3240

auugccugcg caacucgaga gcacaucaac uauaugaaua accaacacgc ucuggauaag     3300

agcaaggaca aaaaggaaaa acagcagucc cggauugauu ugcauccucu guga           3354


<210>  2772
<211>  3354
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2772
augcagacca agaaccugcu cuacacacuu ggccuggaca ucgggauugc cagcguggga       60

uggugcgucu ugggugagaa uagaauaauc gauuuaggcg uuagguguuu cgacaaagcu      120

gaaacucccg aucgcgggga gucccugaac aaggugcggc gagacgcacg ucuaaugaga      180

aggcggcucc gcagaagggc cuggcggcug acgaaacuug cgcgccuguu gaagcacgcu      240

ggacucauug auucuacccg aaucguaaga gacaggcaug auggcgccgu gucaccuugg      300

caacugcggg ucgaaggucu ggaccguaaa cucgcaccag aggaaugggc ccgcgugauu      360

uaucacaucg uuaagcauag aggguuucac uggauaagua gggcugagga gcggaaggca      420

gaaagcgaua aaggagaggg cggcaaggug aaacaggggu uauccgccac aaagcagcug      480

auggucgaga aaaauuaccg aucugcugcc gaaauggugc uuacugaguu cccgggagcg      540

cagcgcaaca agagagguga auauaccaag gcauugucga ggauccugcu agccgcugag      600

cucacaaccc uguuugaacg gcaacaggag cuuggcaauc cccaugccgg gccugaccug      660

cagacuaaaa uuuugggaaa cggcgaucgc aagucagguc uccuguggau gcagaaacca      720

cccuuaagcg ggagugcacu gcucaagaug cuuggaaaau gcacauucga aagagaggag      780

uacagggcuc cuaaggccuc cuuuaccgcg gaacgucacg uauggcugac gaaauugaau      840

aaccuacggg uucuggugga cggcgucacu cgaccacucu cugaucccga gagaaggcug      900

gcccuuagcc ugccuuauca acaggcaucc gacuucaccu acaagcaguu gcgcgcugcc      960

cucuguaaga auggccuguu accagacuca uuucaguuca gugggcuggc uuauccgaca     1020

ggagcccaac agcccgaugg uaaagcaaag aacccugaaa augaccggcu agugaaacuc     1080

ccagccuggc aggaguuuag aaagacucug agggaugcug gccuugaaac cgagugggag     1140

agcaucgcgg ggauggcagc cggaggccaa cccgacuugc uggaucagau ugguugggug     1200

cucucugucu ucaaagacgg ggaugaaguu cugcgcgagc uucgaaagau cccucugcca     1260

ggcggagaaa agauggugca ggcuuugucc ggcguaucau uugacacauu ccacaaccuc     1320

agccugaaag ccuuacggaa gauagugccc cauauggagg cagggcugcg uuacgaugaa     1380

gccugccuag cugccggaua ccaccauagu gaccucagaa aagauaccga cgagaggucu     1440

cuguaucuuc cgccuuugua cuccggucgc acgacugacg gccggauggu cuuuagagau     1500

gacauugaug ugcccaggaa uccaguugug cugcgagcaa ucaaccaggc gcgcacaguc     1560

gugaauagcg uaguucggag auaugggucg ccuucuucca ugcacauuga gauggcuagg     1620

gaccucucac gucccuggga ugaacgcaac aaggugaaac gggcccaaga ggaauacaga     1680

gacaggaacg agaagcugaa gaucgauuuc gcagccgagu auggaaucga accaaaaagu     1740

cgagaguucg aaaaguuuuu acuguaccgg gagcagggcg guaaaugugc uuauagccuu     1800

aagcccuugg accucgaucg cguccugaga gaccuagggu acgccgaagu ggaucacgca     1860

cugccuuacu ccaggucuuu cgacgauagc aaaaauaaca agguccucgu gcuggcugag     1920

gagaaucgga acaagggcaa ucgaacccca uaugaauacc uugacggaac uucagagagu     1980

ccgagauggc gcgaauuuaa agccuucguu gagacaaaca aggcguauag guuggccaaa     2040

cgugcucggc uguuaagaaa ggacuuuucc ggcaaaggga gcgaagaguu cagggaucgc     2100

aaucucaacg acacccgaua cauuucuaga uuuuucaaga auuaugugga gcgguuucug     2160

aaacuugcag aagagucaga agagaggacg ugcguagucc ugaguggaca guugaccgcc     2220

uuccuccgcg cucguugggg ucuggccaag gugagaggcg auagcgaccg gcaucacgca     2280

cuagaugcgg ccguuauagc ugccuguucc aggucuaugg ugaagcgccu gucggacuac     2340

gcacgaagac augaacucga gaaaguccgg acaggguuug uggauuucga gacuggagaa     2400

aucguagacg cuggcaagua uaggcagauu gagcgcgauu uucccagccc uuggccauac     2460

uucagagacg aacugcuuac cagguugaac acagaugacc ccgccgagcu gcgggagcgu     2520

augcaauccu uagguucaua cccuccagaa gcacucgaga cucugcgccc cauguucgug     2580

agucgagccc cgcagagaag gaaugggggc gcugcgcaca aagauaccau cuaucggcag     2640

acagaacgcc uuaagaaagu uggaucuguc acccagaagg ugccucuggc cgcacuaaac     2700

cucagagacc uggagaauuu gguggaccca cauaggaacg aaaagcugua cgcugccaua     2760

cgggcccgau uagaggcuca cggcgggaaa ggagagaagg cauuuccccc uaauaacccc     2820

cucagaaaac cagaucgcga cggcaacccu acugguccca uugucaggac gguuacacug     2880

gugaucgaua agaugagcgg gaucccagug cggggaggcg uagccaaaaa ugacaccaug     2940

cuucgugucg auauuuucag auccaagaag gacgguaaau uucaccuggu gccguuguau     3000

guucaucacg cgguggcuaa gcaacucccu acuagggccg ucaucgcaua caaagaugaa     3060

ggcgagugga cccccauuaa caaucaugac uucgauuuuc uguucgcccu auauccaaac     3120

gaccuggugc gcauaacacu uaagaaagaa cggcacuuug gguacuaugc uggaugcaau     3180

cgagcaaccg gcgccguuuc aaucugggcg cacgauagaa accagaggau ugggaaggac     3240

ggauucaucg acggcaucgg ugugaagacu gcucucagcg uagagaaauu ucaugucgau     3300

acacugggga auguguaccc cgccccucca gaaacgcgcc ggggauuggc auga           3354


<210>  2773
<211>  3357
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2773
augggcacca gauacagcuu cgacaucggg acaaacucca uuggaugggc cguguggagg       60

acuggucggg aucccaaggg cauauuuggg gagaaagacg cuccucugga acuccuugga      120

gcaggcgucc gccuguucaa ggauggucga aauccaaaag acgggcaguc uuuggccacg      180

augcguagaa ucccgaggca agcgcggaag cgcagagaua gguuuguuuu acggcgcgag      240

gcucuggugg ccgcacuaau ugaagccgga cucaugcccg cuucagagaa agaacgaaag      300

gcacuggccg accuugaucc uuaucagcug agagcugccg gcuuggacaa ggcgcucgca      360

ccacacgagc ugggcagggc caucuuccau cugcaccagc ggcguggguu uaaaaguaac      420

cgcaagaccg auagaaaaag cgacgauagg gacaagggaa aaauugcuac agccuccgca      480

gcucucgcca agaaguucga ggaugaaaau gcgcggacuu uuggugaguu cuuauggcga      540

cgccauagag accccgacau gaaaaggcag gccguacuga aggcacacga uccugcuaaa      600

ggcaagccag accuucgggc cgcuaaauug cgccaaccca ccagaaucag gcuggaaggg      660

cagggagcca cagcacuaua cgaguuuuau ccuacccgug agaugcucug ccaugaguuc      720

gaugcccugu gggaagcuca ggcgcggcac cacccaaugc uucugacucg agaagcaggc      780

gacaagauaa gaucuaucuu guuuaggcag cgcgagcuca aaccgccccu gauugguuua      840

uguacauucg aauacgccgc uggggagaag gagucgcggc ugccuaaggc ccucccauca      900

gugcaagcaa gagaaaucua ugagaggcuu ggacauauuc gccugagcac cggcaguggc      960

acggaucgac ccuugaaauc cucugaacgg gacgaucuag ccacugcucu gcuccacugc     1020

gggcugacca agaucaaagu cagcaagaca aaaggacuug ccuuuaagac ugaguuccgu     1080

aaggcacuga gauugggugg cgaagugagg auaaacuucg aggagauggg ggaagaggga     1140

cucguugacc ugcgcaccaa ucgguuacug gugcaggaug accauuacgg cccuagaugg     1200

agggcgcuau ccuggucagg uaaagaugcu uuugucaaga aacuccugga agccacagac     1260

gacgauaaac uuguggcacg auugaugacc gaggacgggc ugagugccgu acaggcucgc     1320

aacgccgcaa gcauuccacu ccccgauggc uauucucggc ugggaccgac ggcuaaugcc     1380

gcgauccuuc aggcccugau ugacuccaga gaugaaaacg gcuucguugu gacuuacgac     1440

aaggcuguca ggcgugcagg ggagcgcuug ggaaaggauu ggcaccacuc agacgagcgg     1500

gauggugaaa uccucgacgc ccugccuuac uauggcaagg uguuacaaag acauauccug     1560

cccgggauga uggauccagc ugacaggaaa gaugaggcca aguuuuacgg acgaauuaug     1620

aauccuagcg ugcacauagg ccuaaaccag cuccggcgcc uggucaaugc acuuaucgaa     1680

agauucgguc ccccagacca gauuguugug gaguuggcga gggaccugaa aaugagugcc     1740

cgggagaagg aacaagagca gcgaagaaac cgcgauaaua gggaagcuaa cgagaaacgu     1800

acaaagaagc ucguagaacu ggggcaggac aauaacggcg agaaccgggc cagauuaaaa     1860

cuguaugaug agcagcaaag ggcaggaaau ggcguggcuc auugucccuu uacccuugac     1920

ccuaucggga uaacucaguu guucucucca gaaauugaga ucgaacacgu ccucccgcgc     1980

ucccgaacac uggaugacag cgccgcaaac aaggugcuau gcuuuagaac caucaaucgg     2040

auuaaaaggg gaaagacgcc cugggaggcc uuccaugcug auccuaaaug gccagacauc     2100

gcggccaacg cagaaaagcu gcccccuaau aaacgcuggc guuuuagaga ggaugcuaug     2160

gacguuuucg aggcccggac cucggguaag auggauaaga caacucucga acagaugggc     2220

cugaccacag gguuucaggc caggcaccuu aacgagacua aacacauguc ucgcuuggcu     2280

aaggcauacc ugggaaaaau uugugaccca gaugccgugu augucacccc cggcgcguua     2340

acaucccucc ugcgagguaa gugggggcuu aauucaauac ugagugacga caacuugaaa     2400

gauagaaccg accaucggca ccaugcuauc gaugccauua cuaucggcgc aaugacgagg     2460

agccuccugc uagaacugaa uacacucgcc ggaaaggcug agcaauccga cuacgaugac     2520

gugguaggca agguuccgug gccuuucgau aacuuucgcg acgcagugag aucugucgug     2580

gauagggucg ugguuagcaa uaaaccagaa cacgggaagc agggagcccu gcacgaggac     2640

accgcguaug gcaucguggg ugaugaggcu gaagccgagg aaauugggaa ccuuguacgg     2700

cguaaauugc uggucgacuu aacucccgga gagguggacc gcauacgaga ugcaggccuc     2760

agaaaggcca ugcaggcugc cguugcaccu ucaagggacg cucgggguaa agugagugau     2820

gccaagggcu ucgcggccgc acuggacacc uucgcuaagg cccaggggau gcgcagaauc     2880

agggucggaa aagaggauau guccgcugug acaauucaug accggcaaac cgauaaggca     2940

uacaaagccg uagcccccgg cgaaaaucac cauguggacg uuguccagau gcgagauggg     3000

gcuuggaagg gauuugcggu gacuguguuc gaggcaaacc agaaagacuu uagaccacag     3060

ugggaacgcg agaaacuugg cgguaagcug gucaugaggc uacacaaggg ggaugccguu     3120

gaacucgaga gcucuggagu gcggcuggug aaguucguac aacagaucug gaugaaauca     3180

aauuuggucc uguuaacaga gcauaacgaa ggcggugacc uccagaagcg ucaccaagau     3240

gcugacgacc cuuuuagaug ggauuucgcc aacauuggca aacugaagga gagggggugc     3300

guggcaguua aaguggacga acuuggacgc gucacgaagc ggaucaguaa uaccuga        3357


<210>  2774
<211>  3360
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2774
augggcuaca gaaucgggcu ggacguggga auuaccagca cagguuaugc cguccucaag       60

acugauaaaa acggccuucc cuacaagaua cugacguugg acuccguuau cuauccuagg      120

gcugagaauc cacagaccgg ggcaucuuua gccgaaccgc ggcgcauuaa acgaggacug      180

cguagaagga cacggcgcac uaaguucaga aaacaaagga cccagcagcu auuuauccac      240

ucaggccucc ugaguaagcc cgagauugaa cagauccuug cgacaccuca agcuaaguac      300

agcguguaug agcugcgggu agccgguuug gaucgccgac ucaccaacuc cgaacuguuc      360

agagugcugu acuuuuucau agggcauagg ggauuuaaau cuaaucggaa ggcagagcuc      420

aacccagaga augaagccga caaaaagcag augggccagu uacugaacuc gaucgaggaa      480

auucguaaag cuaucgcaga gaagggcuau cgcacugucg gggagcuuua cuugaaggau      540

cccaaauaca augaccacaa gagaaacaaa ggauauauug augguuaccu gucaacaccu      600

aauaggcaga ugcuagugga cgaaaucaag caaauacucg auaaacagcg ggagcugggc      660

aacgaaaaac uuaccgacga guucuaugcc acguaccugu ugggggauga aaaucgagcu      720

ggaauuuuuc aggcccagcg cgacuucgau gagggcccag gugcggggcc cuaugcagga      780

gaccaaauca agaagauggu uggcaaggac auuuuugagc cuacugaaga uagagccgcu      840

aaagccaccu acacauucca guauuuuaac cuccugcaga agaugacuag cuuaaauuac      900

caaaacacca caggcgacac cuggcauacg cugaaugggc ucgauaggca ggcaaucauc      960

gacgcugugu ucgccaaagc ggagaagcca acuaaaacau acaagccgac cgauuuugga     1020

gaacuucgga agcuguugaa acuacccgac gaugcccgcu ucaaccuggu caacuauggu     1080

agucuccaga cucagaagga gauugaaaca guggagaaaa agaccagauu cguagacuuu     1140

aaagcauacc acgaucuggu uaaggugcuu ccugaggaaa uguggcaauc caggcagcug     1200

uuggaccaua uaggcacggc ucucacccug uauucuagcg auaagcgucg gcgaagauac     1260

uucgccgagg aauuaaaucu gccagcugag cuaaucgaaa aacuccugcc ccuuaacuuu     1320

uccaaguucg ggcacuuguc aauuaaaagu augcagaaua ucauaccuua ucuggagaug     1380

ggacaggucu acagcgaggc cacaacuaac accggcuaug acuuuaggaa gaaacaaauu     1440

ucuaaggaca caauccgcga agagaucacu aauccagugg ugcggagagc agucaccaag     1500

acaauuaaaa ucguugaaca gauuauaagg cgcuacggua agcccgaugg gaucaacauu     1560

gagcucgccc gagagcuggg ccggaauuuc aaagaacgug gagacaucca gaagagacag     1620

gauaaaaaca ggcaaaccaa ugacaagauc gcugcggagc uuacugaacu gggcauuccg     1680

gugaacgggc agaauauaau ccgcuacaaa uugcacaagg agcagaacgg aguagauccu     1740

uauacgggug accagauucc cuuugaacgg gcauucuccg agggcuacga gguggaucau     1800

aucauuccau auucaaucag cugggacgau aguuacacaa auaagguccu caccucugcc     1860

aaaugcaaca gagaaaaggg gaauaggaua ccuauggugu aucuggcuaa caacgagcaa     1920

cgauuaaaug cccugacuaa caucgcagac aauauuaucc gcaacucccg gaaaagacag     1980

aagcuacuca aacagaagcu gagcgaugag gaacuuaagg acuggaaaca aaggaauauu     2040

aacgauaccc guuuuaucac acgcguuuug uacaauuauu uccggcaggc cauagaauuu     2100

aaccccgagc uggagaagaa acagagagug cucccacuga auggagaagu caccucgaag     2160

auuaggucuc gauggggcuu cuuaaaagug cgggaggacg gugaccugca ccaugcuauc     2220

gaugccacug uaauugcagc gaucacaccc aaguuuauac agcaaguuac gaaguacucc     2280

cagcaccagg aagugaaaaa caaucaggcu cuuuggcacg acgccgagau caaggaugca     2340

gaauacgccg cugaggccca acgcauggac gcagauuugu ucaacaaaau uuucaauggg     2400

uuuccucucc cauggccgga guuccuggac gaacuacugg cuagaaucuc agauaacccc     2460

gucgagauga ugaagaguag gagcuggaau accuauacuc cuauugaaau cgccaaacuc     2520

aagccagugu uugucgugcg gcuggcgaac cauaagauau ccggccccgc ccaccuugac     2580

acaauucgau cugcuaaauu guucgaugag aagggaaucg uucugagcag agugucaauc     2640

accaaauuaa agauuaacaa aaagggccag guagcaacag gggacggaau cuacgauccu     2700

gagaauagua acaaugguga caaagucgug uauuccgcca uucgccaggc ucucgaagcc     2760

cauaacggca gcggggagcu ggcauuucca gauggauacc uugaauaugu ugaccacggc     2820

acuaagaagc uggugaggaa aguccgugug gcgaagaaag uaucuuugcc cgugcggcuc     2880

aagaauaaag ccgcugccga caacgguuca augguuagaa uagaugucuu caauaccggg     2940

aagaaguuug uguucgugcc gaucuacauu aaagacacgg ucgagcaggu ucugccuaac     3000

aaggcaaucg cuaggggcaa aagucuaugg uaucaaauua ccgaaagcga ucaguuuugu     3060

uucucccugu acccaggaga cauggugcau aucgagucua agacaggcau aaaacccaag     3120

uacucgaaua aggagaacaa uacuagcgug guaccuauca aaaacuuuua uggguacuuc     3180

gauggagccg acauugcaac cgccuccauc cucguccgcg cucacgauuc aaguuauaca     3240

gcgcgaucua uuggcaucgc cggucugcuu aaguuugaaa aauaccaggu ggacuauuuc     3300

gggagauacc acaagguuca ugagaaaaag cggcaauugu ucgugaagag ggaugaauga     3360


<210>  2775
<211>  3360
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2775
augaacgaca agaaaaaucu gggcccccuc accuucgggu uugauaucgg aauugccagc       60

guggguuggg cuguccuuuc cccuacaaga auaguugacc ugggcgugag gugcuucgau      120

aaggcagaga cugccaaaga aggggagucu uugaacuuag cgcggcgcuc agcucgacug      180

augcguagaa ggcuacggcg cagagccugg aggcucauca agcuggcacg gcuucugaaa      240

cacgaaggau ugauugccga cacgaagacc uuuauccagc caagugcuag ccaugcaucc      300

ccguggcaac uccgcaagga uggccuggac cgagcccugu cucccgagga augggcuaga      360

guacucuacc acuuauguaa acauaggggu uuccacuggg ugucgcgggc cgaggcgcgu      420

aaggcagaug gggacacaaa aggagagggc ggcaagguca aacaggggcu ggccucaacu      480

accaaacuua ugaaugauaa gagcuaucgc acagcugccg aaaugguguu ggcagaguuu      540

ccuagugcuc agagaaacaa gaggggagaa uacaccaagg cccugucccg ggagcuacuc      600

gacaaugagc ugaaacagcu uuucgaacga caacgcgagu augguaaccc acaugcggau      660

acuaaucugg aagccaagau uagaggcucu ggggaccaca aaagcggauu guuuugggag      720

cagaagcccg cacucuccgg caacgaucug uuaaaaaugc uggguaagug cacauucgaa      780

aagcaggagu acagggcucc uaaagccuca uuuaccgcug agcggcacgu uuggcucacg      840

cgccuuaaua accugagaau cauaguggac gggaggacuc guccauuggc cgacgcagaa      900

cggaccaugg cccuaagucu gcccuaugag caggucggag auuucacaua caagcaacuc      960

cgagcugcgc uggugaaagc aggccuuaug ccugacagcu uuaaguucgc cucucuggcu     1020

uauccauccg aauuucagaa agccgagggc aaggcaaagg auccggaauc agccgcuuug     1080

acuaaacucg ccgcauggca ggagcugaga gcgaccuuaa agaaagcuga ccuggagagc     1140

gaauggcagc aaaucaguug ugccgcacua gaggggcagc ccgaaacacu cgaugagauu     1200

gccggaguac ugucuguuuu caaggacgau gacgaagugc uuaaugaguu gcagaaacug     1260

ggucucccug gcggggcuaa gaccgagaac gcccugcuug auaucagguu cgacaaguuu     1320

caugcacugu ccuugaaagc ucuccgcaag auugucccau acauggaacg gggacugaga     1380

uacgaugagg ccugcgaaag ggcggaguau caccauagcc aauuacacaa agugggcgag     1440

gguaaucgca aguaccugcc cccucuauau aaaucgcgag acaaggacgg gcggauggug     1500

uucaacgaug aacucggcga caucccacgu aaucccgucg uucugagauc ucuuaaccag     1560

gccaggaagg uguugaacgc ucugauagca cgcuacggau ccccgucaag uguacacauu     1620

gagauggccc gggaacucag caaaccuuuu gaugagagaa ugaagguggg caaagaacag     1680

gaggcuuaua gggagcgaaa ugaaaaggac aaacagacgu ucgucgccga guuugggauc     1740

gugggagcag cgaagggugg cgaguucgaa aaauggaagc uguaccgcga acaacagggg     1800

aagugugccu auuccauuca gggaaucgau uuaaaccggc ugcuugagcc cggcuacguu     1860

gagaucgacc augcuuugcc auacucuagg agcuuugaug acucaaaaag uaauaaggug     1920

cucguccugg ccgcagaaaa cagaaauaaa gguaaccgua cuccuuauga guaccuagau     1980

ggggaacaca auuccgagcg cuggcggcag uucgugggcu uuguagaaag caacaagcug     2040

uauagacaag cuaaaagguc ucgacuccug cggaaggacu ucggaaagga gcaggccgau     2100

gaguuucgcg aaagaaaucu uaacgacaca agguacguuu gccgguucuu uaaaaaucag     2160

guggagcagu ucuugaagcu ggcagaaggc ucagagagua aacgaugugu cguguuaagc     2220

gggcaacuca ccgccuuccu gagagcucgc uggggacuua uuaaggucag ggaugagucc     2280

gaccgucauc acgcgcugga cgccgugguu guggcagcuu gcacucaugg uaugguaaaa     2340

cggaugucug auuaugccag aaggaaggaa uuggaccagg ucgccgaggg cuuuguggau     2400

guugaaacag gggagauaau caacgcugca auguuccagc agcuccacga acacuuuccc     2460

gacccauggc cccauuuccg cgaugaggug caaaugcgac ugaagauuga cgauccugcc     2520

cagcuaagau cggaccugcu ccggcuggga aauuacgcgg aggaagaugu ccacgcugug     2580

aggccacuuu uuguaagccg cgccccgcag cguagaaacu ccggcgcagc ccauaaagag     2640

accaucuaug gucaacccga acgguugaag gcucaagggg gcgugacgga gaaaguugca     2700

cugaccgccu uaacaaugaa ggaccucgau aggcugauag acccucaccg caaugaaaaa     2760

cuuuacgcgg cuauucgaaa gagacuggag gaguucggag gcaaggccga uaaagcauuu     2820

gccgcuucaa acccacuaca caagcccgac aaagaaggga acccuacugg accaaucguc     2880

cggaccguga cacucgagau cgacaagcug aguggcgugc ccgucagggg ugggauugcc     2940

aaaaaugaua cuauguugcg cguugacgug uucaccaagg caggaaaauu ucaucuggug     3000

ccgguauacg uccaccauaa ggugacaggc uuaccuaaca gagcuaucgu ugccucuaag     3060

gaugaagacg aguggaccgc gauugaugac agcuucgaau uucucuucuc ccuguaucca     3120

aaugaucuug ugacuauaac gcagaaaggu aaggcccccg ucaggggcua cuaugcauca     3180

ugucaccggg ggacaggagc uuuuagccug ugggcccaug accgugcuuc uuccgugggc     3240

aaagggggac agaucgaggg cauugguauc aagaccgcau uggccguuca gaaauacaac     3300

guggauguac ucgggacuac cuaugccgcu ccucuggagc agcgccgagg acuagcguga     3360


<210>  2776
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2776
augagaaagg ugaccaucgg cuucgacauu ggggucagcu ccguuggaug gucuguggua       60

gauaugaaca auggugccau aguggagaca ggcgucaggc uguuuucagc uaguagcgca      120

gaaaacaaug uggagcggcg caacaaucga cagacucgua gacucaacag gcggcgcaaa      180

aauagacuua aggacguuaa auaccuguug gaaacgaacc acuucaccuu ugaucaagag      240

caggggacaa aucccuauga auuaagggug gccggacugc aucggaagcu aacucgcucc      300

gagcucgcgc gagcucugua ccaccuuguc aaaagaaggg gcaucucuua ugaccugacc      360

gauauugagg aagaggaaau cgugacaacc ucggccuaca agacugcauu ggguaagaac      420

cagucacagc ucgccgagaa aacaccuggg gagauucaau ucgcucggcu ggaaaauuau      480

ggaaagguac guggcagcau cgagauaaac gaacaggaca uccugcucaa uauuuuucca      540

accagugcau acuuaaacga ggccaaacgc auccuggcua cgcagagaaa guucuauccg      600

gaaauuacug aggccuuuau cgagaaauac ugcaccauac uuacaaggaa gcgggaauac      660

uauguuggcc ccgggacuga gaagaaucga accgauuacg gaauuuaucg cacaaacggu      720

gaaaccuugg acaaccuguu cgagauccua auuggcaaag auaaguuuua cccuaaugaa      780

gagagagcgg cagggaacuc cuauacggcc caacucuuca auuuugugaa cgaccugaau      840

aaccuuaaaa uccagacuau cgaggaucag aagcugacac caaaacagaa ggaagagauu      900

auagaacagg ucaaaaccgc ugagaagaag gugucuuuga uccaacucau uagcaaaauc      960

acuaagacac ccaaagacca gauaaccgga uacaggacgg auaagaaugg caaaccugac     1020

cugcauucca uggccguguu ccggaagguc cgcaagcagu uuuuaaccau ggaaauugac     1080

auccugcacu ggccagauga gcuccuuaac gaccugggua gauugcuaac acugaauacu     1140

gagaaagggg aaaucaggaa gcaacucacc aaagauuuca ugaagcguua ucccauucug     1200

aacgagcagc uuguugacca gaucauugaa aaucggcaua uauucgcagu gucaggaaac     1260

cacaaauggc accgauuuag ucugagaaca augaaugagu ugcucccuga gaugcuggcu     1320

acuagcaagg aacagaugac cuuacugucu gagaggggca ugaucaagca acauaaacgc     1380

gauuaccuaa agaugaacga acucgacgcc cggcugauuu ccgaggaaau cuacaaucca     1440

guagugggca aaucagucag agaggcgauc aaacuuuuca acgaguugau gaaaaaguau     1500

caggaaguga gguacguugu gcuggagaug ccgcgcgaug acaacgaaga gaaggccaaa     1560

aaggaacucc agaaauauca gaaggagaau gagaacgaaa aagagauugc acugagcgaa     1620

uuucaacgaa agggguucuu uagugaugcu cagcuugagc gggcccugau aaaagaccgu     1680

aaguugaaga caaaaaucag auucuggcac cagcaggaag gaaagugucu cuacucuggu     1740

caaauuauca cccccgucga gcuguuaacu aauuccuuua gcuucgauau ugaccauauc     1800

auaccucagu cggugucuuu ugacgauucc cugaacaaua aaguacuaug cacgaaggag     1860

augaacgacc agaaaggcaa gcagacacca uauggguucc uccugaaugg aaacggccaa     1920

gguuuugaua gguucaaggc uaugguuaau gccaaccgcc uuaugucaaa agggaagaaa     1980

aagaauuugc uguuuaugga agaccucaac aauaucgaga cccggaaaag auucauugca     2040

aggaaccugg uggauacucg auacgccagu cgcgucgugu uaaaugaaau ccagcaguau     2100

cugcgggcua agaacaagga cacccaaguc gcggugguua gaggcaaauu cacagcaacc     2160

cuuaggaagc acuggaacau uaauaaaagc cgugagacuc accaucacca ugccuuggau     2220

gcuuccauca uagccauuac accccuccug ucugugugga agaaaaagca gacgcuaauc     2280

ccuaagaccc ugagcgagga aaaacucgag uuugaauacg acgauauugu agaggacacu     2340

aucuucgaaa aggcacugua ccagcuucca aaagagaacu uuaaggagca guugaauaac     2400

ucacaaccca auauaaaauu caguuaucag gucgauaaga aaaugaaccg caaggugucc     2460

gacgccacaa ucuacagcgu uaagcaggcu aaacugucuu cagauaagca gagugaagag     2520

uauguguuag ccaaaauuaa ggacaucuac agcgucgaag gauauaaaac cuuugcaaag     2580

auuuacgaga aggacaaauc caaguuccuc auggcgcaaa augauccgaa aacauuugaa     2640

aagcuggagg agaucauaca gucuuauccu ucgacucggg aaauuaucgu gggcgagaaa     2700

guaaaggugg uugacgucag ccccuucgaa cuuuacagaa aggaguacgg guauaucacc     2760

aaauacgcua agaaaaacaa uggaccaauu aucaggcagc ugaaguauua cgaucacaaa     2820

auugguaugc auuuggacau aacgaaccag aagaauaacg gcaaggggaa aaaggugauc     2880

cuccaacagc ugaaauccug gcgaaccgau guguauuaca aucacgaaaa gcaggaguau     2940

gagauuaugg gacuaaaaua cucagaccug cgguuuaaga aggaucaaua cggcaucccu     3000

aaagaacgcu aucaggaggu caagcagaug gaaaagauug caccccagag ugaguucugu     3060

uuuucucucu acagaaacga caggaucaag guucugaaua aagagacaga ugaagaggug     3120

gaacuuuugu ucggugcccg gaacuauacu agcgccgggu acguggaccu gaagccaaua     3180

gaucgaguag aguuuggcaa aaaggaaugg auccccgucu aucaauacgc uaccuccgac     3240

ggaaagugca aaaagaaauu cgccaagaaa ggcuaugaga uuuacaaggu gaacacaacu     3300

aucuuagggg agucauacua uauuaagaaa gaaagaaugc ucccuaagaa uauccuggau     3360

uga                                                                   3363


<210>  2777
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2777
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu ugguaaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggcgcg ugagacugaa uaggcuauuu gaggaaauug gccucaucac ggacuucacc      240

aagauaucaa ucaaccugaa uccuuaccag cuucggguca aaggucugac agaugaguug      300

aguaacgaag aacucuuuau ugcccugaag aauaugguga agcaccgcgg gaucagcuau      360

cuggacgaug cguccgacga uggaaacucu ucgguuggcg acuacgcuca gauugugaaa      420

gaaaauucaa agcagcucga aacuaaaacc ccaggccaaa uccaguuaga gcgauaucag      480

acauacgggc agcugagagg agauuucacc gucgaaaagg acgguaaaaa gcauaggcuu      540

auaaacgugu uuccgacuag cgccuaucgg agugaggcau ugcguauucu gcaaacacag      600

caggaguuca auccccagau caccgaugaa uuuauuaacc gcuaccuaga gauccucacg      660

ggcaagagaa aauauuacca cgggccugga aaugaaaagu ccaggacuga cuacggccgg      720

uaucgaaccu cuggugagac acuggauaac aucuucggga uucuuauagg aaaaugcacu      780

uuuuacccag acgaguuccg cgccgcuaag gcaagcuaua ccgcccaaga guuuaaccug      840

uugaaugacc ucaacaaucu gacaguaccc accgagacga aaaaguuauc caaagaacag      900

aagaaccaga ucauuaauua cguuaagaac gagaaagcua ugggcccugc caagcuguuc      960

aaauauaucg cgaagcuccu uucaugugau guggcagaca uaaaaggcua cagaauugau     1020

aagaguggga aggccgaaau ccauacuuuu gaggcuuaua ggaaaaugaa gacacuggaa     1080

accuuggaca ucgagcaaau ggaucgggag acucuagaca aacuggccua cguccucaca     1140

cugaauaccg aacgcgaggg aauccaggaa gcacuugagc acgaguucgc ugaugguagc     1200

uucucucaga aacaggugga cgagcuggug caauuuagaa aagccaacuc cucaauuuuc     1260

ggcaaggggu ggcacaauuu uagcgucaag uugaugaugg agcucauucc agaacuguac     1320

gagacgagug aagaacagau gaccauauua acaaggcugg gaaaacagaa gacuaccucu     1380

uccagcaaca aaacaaagua uaucgaugag aaacuacuca cugaagagau uuacaauccc     1440

guuguggcga agucgguacg ucaggccauc aagaucguga acgcagcuau uaaagaauau     1500

ggcgacuucg auaauauagu caucgagaug gcccgggaaa ccaacgagga cgacgagaag     1560

aaagcuauuc aaaagaucca gaaagccaau aaggaugaaa aagacgcagc caugcugaag     1620

gcugcgaacc aguacaacgg uaaggcagag cuuccucauu cuguguuuca cgggcauaaa     1680

caguuggcca caaagauucg acuguggcac caacagggcg aaagaugccu cuauaccgga     1740

aaaacuaucu ccauacacga ucugaucaau aacccaaauc aguucgaggu ugaccauauu     1800

cuuccgcugu caaucacguu ugaugacagu uuggcuaaca aggugcucgu cuacgccaca     1860

gcaaaucagg aaaaaggcca aaggaccccc uaucaggccc uggauagcau ggacgaugcu     1920

ugguccuucc gcgaguuaaa ggccuuugug cgggagucua agacucugag caacaaaaag     1980

aaagaauacc uacucaccga ggaagacauu ucaaaguucg auguaagaaa gaaguuuauc     2040

gagaggaauc ugguugacac acgcuacgca agucgagugg uccuuaacgc guugcaggaa     2100

cacuuccggg cucauaagau agauaccaaa guguccgucg ugcgugggca auuuacuagc     2160

cagcugagaa ggcacugggg aauugagaag acacgcgaca cguaucauca ccacgccguu     2220

gacgcacuca ucauugccgc uucuucacag cugaauuuau ggaaaaagca gaaaaacacc     2280

cuggugaguu acagcgagga ucaacuuuug gacaucgaaa cuggugagcu cauauccgau     2340

gacgaauaua aggagucugu auucaaggcc ccuuaccagc auuucgucga uacacugaaa     2400

ucgaaggagu uugaagacag cauccuauuc uccuaucagg uggauucaaa auuuaaucgg     2460

aagauuagug acgcaaccau cuacgcuaca agacaggcca aaguuggcaa ggauaaagcg     2520

gacgagacuu augugcuggg gaagauuaag gauaucuaca cccaagacgg auacgaugcc     2580

uucaugaaaa uauauaagaa agacaagucu aaauuucuca uguacaggca cgacccacag     2640

acguucgaaa aggucauuga gcccauccug gaaaacuauc cuaauaagca gaugaacgag     2700

aaaggcaagg aggugccaug uaaucccuuu cuuaaauaca aggaggagca ugguuauauc     2760

cgaaaauaca gcaagaaggg gaacggcccg gaaauuaaau ccuugaagua uuacgauuca     2820

aaacugggaa accacaucga cauuaccccu aaggauagca auaacaaagu aguguuacag     2880

ucucucaagc ccuggcgcac agacguuuac uucaauaaga acacuggcaa auaugagaua     2940

cuggggcuua aguacuccga uaugcaauuu gaaaaaggaa ccgguaagua uaguaucagc     3000

aaagagcagu acgagaauau uaagguccgg gaaggcgugg acgagaacuc ugaguucaag     3060

uuuacacugu auaaaaauga uuugcuccug cuaaaggacu ccgaaaacgg ggaacagauc     3120

cugcucagau ucacuucaag gaaugauacc aguaaacacu acguggagcu gaagccauau     3180

aaccgucaaa aauuugaggg aagcgaauac cuuauuaagu cguugggcac ggucguuaaa     3240

ggugggcgcu gcaucaaggg ccugggaaag uccaauauau cuaucuacaa agugcggaca     3300

gacguguuag gcaaccagca uauuaucaag aaugaggggg auaaaccuaa gcucgacuuc     3360

uga                                                                   3363


<210>  2778
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2778
augggccugg acaucggggu ggccagcguc ggauucuccg uucucgauau ugacaagggu       60

aaagugauag agcuuggcgc uagacuguuu ucugcaaccg uagccgcggg gaaccaggau      120

aggcgggaca ugcgcggagc ucgacguuug uuaaauagaa acaagcaaag gcggcaggau      180

acaggcaaac uguucaagaa auuuggucua aucgacgauu ucgacaaggg gucauuuuac      240

gauaauuuca accagaaucu caaccccuau gaacugcgcg ugaagggacu uacugagcag      300

cugacgaaag aagaguuggc cgaaagucuc uaccaaauug ucaagcacag aggcaucagc      360

uaugcacuga aagacgccga uguggacgag ggcgggaccg auuacuccgu uucucugaag      420

auuaauucgc aggagcucgc ugagaaaacu ccugcacaga uccaguuaca aaggcugaac      480

gacuauggaa aggugcgggg ucaggucgug auaggcgacg auccagacaa ucagaaggua      540

cuuuugaacg uuuuuccgac aucagccuac gagaaagaag cuaagcagau cauugccacc      600

caacagcagu ucuaucccga gagccugaca gauaaauuua ccgaggaaua cugccaaauc      660

cuaacucgca agcgagacua cuucgugggg ccuggaaaug agaaaaguag aacagauuau      720

ggcauuuaca agaccgacgg uaggacgcuc gauaaccugu uugaagaacu uaucgggcau      780

gacaaaauau auccagaaga acugcgggcg uccgcagccu cuuacacugc ucaguuguuc      840

aauguccuca acgaucugaa uaacuuacgu auucugaacu augaggacgg aaagcucacc      900

aaggaagaua aagagaagau cauugccgaa aucaaaaaua acacaacuac caucaauaug      960

cuuaacguga uuaagaaagu ggcaggcugu agcaaggacg acauaaaggg cuuucgcguc     1020

aaugagaaag auaagcccga aaucuccuca augccuguuu acagaaaaau ucacaaggac     1080

cuguugaaag cuggggugga uaucagugac uggccaguag aguucauaga ugagcuuagc     1140

uucauucuga cacucaacac cgaaaaugga gagaucagga agcagcugaa caaucggcuu     1200

gcccccaagu uugacuuccu gaacgcggau uugauccagc ucauuaucga caauaaagau     1260

ucuuuugaaa uuaagacgaa caauaaaugg caucgauucu ccgugaagac uaugaacaaa     1320

cugauaccug agaugaugga acgcccaguc gagcaaauga cauuacugaa ugagaugggu     1380

cuagugaaga aggacaaaaa gagauuugaa aacaacaaau aucucccgua caaggagauc     1440

gccaaagaua uuuucaaucc cguugcauca aagagcguga gggaagcucu gaaaaucguc     1500

aacgccgugc uuaagaagua cggccacauc gacuauuugg uaguugagau gccucgggau     1560

aaaaaucuga aggaggaaca ggacaacauu aaagaguuuc agaauaagaa caaaaaggcu     1620

aaggacgccg cauucgaagc cuuugugaaa agugucgggu cugagcagcg cgugaaggaa     1680

gcucucucca aaaauagaaa gcugcaaaug aaaaugaggc uuugguacca gcagcaggag     1740

auagauccau auaacggaaa gaccaucgac gcgacugauc ugauuaauaa ccccgacaag     1800

uucgagaucg aucauauuau cccucaaagc auaucguuuu acgacucuau caauaacaaa     1860

acauugugcu ucgcauccau gaaucagguc aagggccaga aaaccccaua ugaauuuaug     1920

cucgaggguc acgggcaguc auacgauaag uucaaagcca cggugauggc uaacaagaau     1980

uucggcaagg ccaaacgugc aaacuaucug uuugaagaga acguuaguga cauugaaacc     2040

cggaagcgau ucuuaagcag aaaucuggug gauacaaggu acuccucucg cguaguccua     2100

aacagccucc aagacuuuuu ccgggagaaa ucagccgaua cuaaggugac cguuaucaga     2160

ggaaaauuua caaguaauau gaggaagcac uggcauauug acaaaacucg cgagaccuuc     2220

caccaucacg cuaucgaugc cuccauaauu gcagcgacac ccuuucugcg aauguggaag     2280

aagggcggga ccaucuuccc ggugaaaguc ggagaagaga gcauugacau cgaaacuggu     2340

gagauacuug acgauaagaa cuuugacaaa gcuauguacg aggagccuua uucuggcuuc     2400

gugucagaga ucaugaaugc cgaugaccgg auuaaguuua gucaccaggu agauaaaaag     2460

augaaccgua aggugagcga cgcaacgauc uacuccacaa gaaccgggaa auuggccaag     2520

gauaaaaagg acgcugaaua uauuguugcc aaagucaagg auaucuacuc uguggacgga     2580

uucaagaaau uuaagaaagu guaugauaag gacaaaacua aguuccugcu cuacaaguau     2640

gaccccagga ccuucucgaa acuggagcgc auaauuagcg auuguccaga caaggucgaa     2700

aaaguucaga caaauggcaa ggugaaagca guggauaucu ccccuuuuga gauguaccgg     2760

agagaccaug guaugaucaa gaaguacagu aaaaagggga acggccccgc uauuaaacaa     2820

uuaaaguauc uggauaaaaa gcuaggauca cacaucgaca uuaccccagc caaugcgaac     2880

ggcaaacaug uaauauugca gucucucaag cccuggagga cugaugucua ccugaaucac     2940

gagacagggg aauaugagau caugggaauu aaguacagcg accuaaaauu caacaagaau     3000

gaagguuaug gcaucaaaaa ggauaaauac cuggagauua agaaggugga agagguuucc     3060

gagaaaucag aauuuauguu cagccucuau cgaaaggacc gcgugaaagu ccaggauaug     3120

aagacggggg agucugugga acuguuguuu uggucccgga acuucaguaa uaaaaaguac     3180

gccgagcuua agccuaucag ccaggcugaa aacgacaaaa agcugccagu uuacggaaaa     3240

ggcagauuaa uaaagaggcu caucccgaaa aacugcaaga uuuggaaggu gaauaccacu     3300

auccugggug aucccuauua ccuugagaaa gagucugacu ccccuaagga cauucuggau     3360

uga                                                                   3363


<210>  2779
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2779
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu ugguaaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggcgcg ugagacugaa uaggcuauuu gaggaaucag gccucauuac ggacuucacc      240

aagaucagua uaaaccugaa uccuuaccag cuucggguca aaggucugac agaugaguug      300

agcaacgaag aacucuuuau cgcccugaag aauaugguga agcaccgcgg gauuuccuau      360

cuggacgaug cgucugacga uggaaacucg ucaaucggcg acuacgcuca gauuguuaaa      420

gagaauagca agcagcucga aacuaaaacc ccaggccaaa uccaguuaga gcgauaucag      480

acauacgggc agcugagagg agauuucacc guggaaaagg acgguaaaaa gcauaggcuu      540

auaaacgucu uuccgacaag ugccuaucgg uccgaggcau ugcguauucu gcaaacucag      600

caggaguuca auccccagau caccgaugaa uuugugaacc gcuaccuaga gauucucacg      660

ggcaagagaa aauauuacca cgggccugga aaugaaaagu cuaggacuga cuacggccgg      720

uaucgaacca acggugagac acuggauaac aucuucggga uccuuauugg aaaaugcacu      780

uuuuacccag acgaguuccg cgccgcuaag gcaagcuaua ccgcccaaga guuuaaucug      840

uugaacgacc ucaauaaccu gacaguaccc accgagacga aaaaguuauc caaagaacag      900

aagaaucaga uaaucaacua cguuaagaau gagaaagcua ugggcccugc caagcuguuc      960

aaauauauug cgaagcuccu uucaugugau guggcagaca ucaaaggcua cagaauagau     1020

aagaguggga aggccgaaau ucauacuuuu gaggcuuaua ggaaaaugaa gacacuggaa     1080

accuuggaca ucgagcaaau ggaucgggag acucuagaca aacuggccua cguccucaca     1140

cugaacaccg aacgcgaggg aauccaggaa gcacuugagc acgaguucgc ugaugguagc     1200

uucucucaga aacaggugga cgagcuggug caauuuagaa aagccaauuc cucaauuuuc     1260

ggcaaggggu ggcacaacuu uagcgucaag uugaugaugg agcucauccc agaacuguac     1320

gagacgagug aagaacagau gaccauuuua acaaggcugg gaaaacagaa gacuaccucu     1380

uccagcaaua aaacaaagua uauagaugag aaacuacuca cugaagagau cuacaacccc     1440

guuguggcga agucgguacg ucaggccauu aagaucguga augcagcuau caaagaauau     1500

ggcgacuucg auaacauugu cauagagaug gcccgggaaa ccaaugagga cgacgagaag     1560

aaagcuaucc aaaagauuca gaaagccaac aaggaugaaa aagacgcagc caugcugaag     1620

gcugcgaacc aguacaaugg uaaggcagag cuuccucauu cuguguuuca cgggcauaaa     1680

caguuggcca caaagauccg acuguggcac caacagggcg aaagaugccu cuauaccgga     1740

aaaacuauuu ccauccacga ucugauaaac aauucaaacc aguucgaggu ugaccauauc     1800

cuuccacuga guauuacguu ugaugacagc uuggcuaaua aggugcucgu cuacgccaca     1860

gcaaaccagg aaaaaggcca aaggaccccg uaucaggccc uggauuccau ggacgaugcu     1920

uggucuuucc gcgaguuaaa ggccuuugug cgggagagca agacucuguc aaauaaaaag     1980

aaagaauacc uacucaccga ggaagacauc aguaaguucg auguaagaaa gaaguuuauu     2040

gagaggaacc ugguugacac acgcuacgca ucccgagugg uccuuaaugc guugcaggaa     2100

cacuuccggg cucauaagau cgauaccaaa gugagcgucg ugcgugggca auuuacuucu     2160

cagcugagaa ggcacugggg aauagagaag acacgcgaca cguaucauca ccacgccguu     2220

gacgcacuca uuaucgccgc uucaagucag cugaacuuau ggaaaaagca gaaaaauacc     2280

cuggugagcu acuccgagga ucaacuuuug gacauugaaa cuggugagcu caucucugau     2340

gacgaauaua aggaguucgu auucaaggcc cccuaccagc auuuugucga uacacugaaa     2400

ucgaaggagu ucgaagacag cauacuauuu uccuaucagg uggauucaaa auucaaccgg     2460

aagaucagug acgcaaccau uuacgcuaca agacaggcca aaguuggcaa ggauaaagcg     2520

gacgagacuu augugcuggg gaagaucaag gauauuuaca cccaagacgg auacgaugcc     2580

uuuaugaaaa ucuauaagaa agacaagucu aaauuccuca uguacaggca cgacccucag     2640

acguuugaaa aggucauaga gccaauucug gaaaauuauc ccaacaagca gaugaaugag     2700

aaaggcaagg aggugccuug uaacccauuc cuuaaauaca aggaggagca ugguuauauc     2760

cgaaaauaca gcaagaaggg gaacggcccc gaaaucaaau ccuugaagua uuacgauuca     2820

aaacugggaa aucacauuga caucaccccg aaggauagca acaauaaagu aguguuacag     2880

ucuguuuccc cuuggcgcgc ugacgucuac uuuaacaaua caacuggcaa guaugagauu     2940

cucgggcuga aguacgcaga ucuucaauuc gaaaaaggaa ccgguacaua uaagauaagu     3000

caggagaaau acaacgacau caagaaaaag gagggcgugg auagcgacuc ugaauuuaag     3060

uucacucugu auaaaaauga uuugcuccug gugaaggaga ccgaaacgaa agagcagcaa     3120

cuauuucggu uccuguccag aacaaugccc aagcagaaac acuacgucga acucaagcca     3180

uaugacaaac agaaguucga agggggagag gcccugauua agguucuugg caacguggcu     3240

aauggugggc agugcaucaa aggcuuggga aagucaaaca uuaguaucua caaagugagg     3300

accgauguac ugggcaauca acauauaauc aagaacgaag gggacaaacc uaaguuagac     3360

uuuuga                                                                3366


<210>  2780
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2780
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaaaauu ccgggaaaau cauucacgcu aacucuagau uguuccccgc agccaccgcg      120

gauaauaacg uggaaaggcg gaagaaucgc caggcucgac guuuaaacag aaggaaaaag      180

caucggggag uacgcuuuca agaccuguuc gaggauuacg gccuacucac agacuuuuca      240

aaagugagua ucaaucugaa cccuuauaga cuuagggucg auggucugaa uaagcaguug      300

acuaacgaag aacucuucau ugcccugaag aauaucguga aacggcgcgg gauaagcuac      360

cuggacgaug cauccgaaga cggaggcacg guuucuucgg auuauggcaa ggccguggag      420

gagaaccgaa aacucuuagc ugaacagacc ccagggcaga uccaacugga gagauuugaa      480

aaguacggac agcuuagggg ugacuucaca gucguggaga auggcgagaa augccgguug      540

auuaacguau uuucaacuag cgcauauaag aaggaagccg agcguauccu gcgcaaacag      600

caggaguuca auaguaagau uaccgaugag uuuaucgaag acuaccuaaa aauacucaca      660

gggaagagaa aauauuacca cggaccgggc aacgagaagu ccaggaccga uuacggucgg      720

uuccgaaaag acgggacuac acuggacaac auuuuuggaa uccuuauugg caaguguacc      780

uucuauccca augaguaccg cgcuucuaag gccagcuaua cggcgcaaga auuuaaccug      840

uugaaugauc ucaacaaucu guccguuccu acugagacca aaaaguuauc agaggaacag      900

aaaaagacaa ucaucgaaua cgcaaaaagu gccaagacuc ugggcgcuag cacccuccuu      960

aaguauauug ccaaaaugau agacacaucu guggaucaga ucagagggua cagggucgac     1020

gugaacaaua agccagagau gcauaccuuc gagguguauc ggaaaaugca gucccuggaa     1080

acgauuucag ucggagaguu gagccgcaac guucuagaug aacuggcaca cauccucacu     1140

cugaauacag agagagaagg uauagaggag gcuauuaaca ccaaacuuaa ggacaguuuc     1200

ucucaagauc aggugcugga auugguacag uuuaggaaga auaacuccag ccucuucucg     1260

aagggcuggc auaauuuuuc ucugaaauua augauggagc ugauccccga acuauacgag     1320

acuuccgagg aacaaaugac aauccucacc cgucugggga agcagaaauc aaaggagacg     1380

aguaaacgga gcaaguacau ugacgaaaag gagcuuaccg aagagaucua uaacccugug     1440

gucgccaaau ccgugcgaca ggcgauuaag auaaucaaug aggccacaaa gaaguacgga     1500

auuuucgaua acaucguuau cgaaauggca agagagaacg acgaagagga ugcuaaaaag     1560

gacuauauua aaaggcagaa ggccaaucaa gacgaaaaga acgcugccau ggagaaagca     1620

gccuuucagu acaauggcaa gaaagaguug ccagauaaca uauuccacgg ucacaaggaa     1680

cuggcuacua aaauccgccu cuggcaucag cagggggaga agugccugua uaccggcaag     1740

acaauuccca ucucugaccu uauucacaau caauacaaau augaaaucga ucaugugcug     1800

ccuuugagcc ucucauuuga cgauagucug uccaacaagg ucuuagugcu ggcgacugca     1860

aaucaggaga aaggacagcg gaccccauuc caggcccuag acagcaugga ugacggcugg     1920

ucuuacagag aauuuaaguc auacguaaaa gagaguaagc uccuggggaa caagaaaaag     1980

gaguaucuuu ugacagaaga ggauauaagc aaaaucgaag uuaagcaaaa auucauugag     2040

aggaaucugg uggacaaccg cuacuccucu cgagucgugc ucaaugcucu gcaggauuuu     2100

uauaaggccc acaaguucga caccacuauc ucggucgugc ggggacaguu uacgagccag     2160

uuacguagaa aauggggucu ggagaagucc agggaaacau accaccauca cgcaguugau     2220

gcccuuauua ucgcugccuc aagucaauug cgccucugga aaaagcauaa caauccgcug     2280

auaucuuaua aagagggccg guucauugac agcgaaaccg gggagaucau uucccuauca     2340

gacgaugaau acaaggagcu gguguucaaa gcacccuaug accacuuugu agauacucuc     2400

agaagcaaga ccuucgagga cucuauccug uuuuccuacc aggucgauag uaaguacaac     2460

aggaaaauaa gcgacgcgac aaucuaugcu acccgaaagg ccaaacuuga uaaggacaaa     2520

ucugaagaga cuuacguguu gggaaagauu aaggauaucu auucccaggc aggcuacgac     2580

gccuucauua aaaucuauaa uaaggauaaa ucaaaguuuc ugauguacca uaaagacccu     2640

caaacauucg aaaagguuau agaggaaauu uuacgcacgu auccaaguaa ggagcucaac     2700

gauaaaaaca aggagauccc cuguaauccu uuugaaaaau accggcagga gaacggucca     2760

aucagaaagu acagcaaaaa ggggaauggc cccgaaauua agucgaugaa auauuacgac     2820

aagaacaucc cggaguccuu cauuaauaua accccuucua acagcaauaa caaagugcug     2880

cuuaggcagc uguaucccug gcguacugac gucuacuuua auccacagac agauaaguau     2940

gaauugcucg gacugaaaua cgcugaccua caauucgaga aggccaccgg caaguauuca     3000

aucacaauug auaaauacaa cgagaucaag gaaaaagagg gggugaguaa ggaauccgag     3060

uuuaaauuca cucuguacaa gaaugaccuc cugcuuauua aagauaccaa gaacaaugag     3120

gaacagaucu uucgcuucuu gucucggacg augccuaacc agaaucacua uguagagcug     3180

aagcccuacg acaaaggaaa cuucgaaggu aaggagaaaa uaaagguguu uaaaaugacc     3240

agcaacggca gaugcaucaa gggguuagga aagaaauauc ucuccauuua caagguuaaa     3300

acagauaucc ugggcaauaa gcacuucauu aaaaaggaag gugacgagcc aaaacuuaag     3360

uuuuga                                                                3366


<210>  2781
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2781
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaaaauu ccgggaaaau cauucacgcu aacucuagau uguuccccgc agccaccgcg      120

gauaauaacg uggaaaggcg gaagaaucgc caggcucgac guuuaaacag aaggaaaaag      180

caucggggag uacgcuuuca agaccuguuc gaggauuacg gccuacucac agacuuuuca      240

aaagugagua ucaaucugaa cccuuauaga cuuagggucg auggucugaa ucagcaguug      300

acuaacgaag aacucuucau ugcccugaag aauaucguga agcggcgcgg gauaagcuac      360

cuggacgaug cauccgaaga cggaggcgcc guuucuucgg auuauggcaa agcuguggag      420

gagaaccgaa agcucuuagc agaacagacg ccagggcaaa uccagcugga gagauuugaa      480

aaauacggac aggucagggg ugacuucacc gugguagaga auggcgagaa gcggcgucuu      540

auuaacguuu ucucaacaag cgccuaucgc aaagaagcug agaagaucuu gagaaagcag      600

caagaauuua auaaccagau uacugaugag uuuaucgaag acuaccugaa aauacuaacc      660

gggaagagga aauauuacca cggaccgggc aacgagaaga gucggacaga uuacggucga      720

uuccgcaccg acgggacuac acucgacaau auuuuuggag ugcugaucgg caaaugcacc      780

uucuauccca acgaguacag agccuccaag ucuagcuaua cggcgcagga auuuaaucuu      840

cugaacgauu ugaauaaccu cacugucccu accgagacaa agaagcuguc cgaggagcag      900

aagaaaguga uuguggaaua cgcaaaggag gccaaaacuu uaggcgcuuc aacccugcuc      960

aaguauaucg ucaaaaugau cgacgccagu guugaucaaa uuagcgggua caggguggac     1020

guaaauaaca agccagagau gcauacauuc gaaguguauc ggaagaugca gucucuugag     1080

accauauccg ucggagaacu gucacgcaau guguuggaug agcuagcaca cauccugacg     1140

cucaacacug aaagagaggg uauugaggaa gcuaucaaua gcaaacugau gggcguuuuc     1200

agucaggacc aagugcuuga gcugguccag uuuaggaaga acaauucuuc cuuguucagc     1260

aaaggguggc auaacuuuuc gcucaagcug augauggaau uaauacccga gcuguacgag     1320

acaucugagg aacagaugac cauucuaacu cgucucggaa aacagaaguc caaggaaaca     1380

ucaaaacgga ccaaguacau cgaugagaaa gaacugacgg aggagaucua uaauccugug     1440

guagccaaga guguucgaca agcgauuaaa aucauuaacg aagccaccaa gaagcacggc     1500

auauucgaca acaucgugau ugagauggca agagaaaaua acgaggaaga ugcuaaaaag     1560

gacuacauca aaaggcagaa ggccaaucag gaugagaaaa acgcugccau ggagaaggca     1620

gccuuucagu auaaugguaa aaaggaacuu ccagggaaca ucuucaaugg ccacaaggag     1680

uuggcuacaa aaauucgccu guggcaucaa cagggagaaa agugucucua cacuggcaaa     1740

accauaccca ucagcgaccu gauucacaac caguauaagu acgagaucga ccauauucuu     1800

ccucuguccu ugucuuuuga ugacagccuc ucaaauaaag uccuggugca cgcgacagca     1860

aaccaggaaa aggggcaacg gacuccauuc caggccuuag auaguaugga cgaugcuugg     1920

uccuauagag aguuuaagag cuacgucaaa gaccugaagu cucuaucaaa uaaaaagaaa     1980

gauuaccucc ugaccgagga agacaucagu aagauagagg ugaagcagaa auucaucgaa     2040

aggaaccuug uugauacacg cuauagcucc cgagugguau ugaaugcccu gcaggacuuu     2100

uacaagucuc accaauucga uaccacuauu ucggucgugc ggggacaguu uacgagccag     2160

cuccguagaa aauggggucu ggagaagucc agggagacau aucaucacca ugcaguugac     2220

gccuuaauca uugcugccuc aagucaacug cgccuuugga aaaagcagaa caauccguug     2280

aucaccuaca aggaaggcca guucauagau ucucagacug gggagauuau cagccucacc     2340

gacgacgaau auaaagagcu gguguucaag gcacccuacg aucacuuugu cgacacacua     2400

cauuccaaaa ccuucgaaga uggcauucug uuuucauauc aaguggacag caaguacaac     2460

cggaaaaucu cugaugcgac uauauacgcu acaagaaagg ccaaacucga caaggauaag     2520

uccgaggaga cguauguacu gggaaaaauc aaggacauuu acagucaggc agguuaugau     2580

gccuucauca aaauuuacaa caaggacaaa aggaaguuuc uuauguauca caaggauccu     2640

cagaccuucg aaaaagugau cgaggaaaua uugcgaacuu acccaagcaa ggagcgcaau     2700

gacaaaaaca aggaaguucc cugcaauccu uuugagaaau aucggcagga gaacgggcca     2760

auuagaaagu acucuaagaa aggcaaugga cccgaaauca agugucugaa auacuaugau     2820

aacaaguuag gcaauuacau cgacauuucc ccggacgggu cagauaacca agucgugcuc     2880

cagagucuga aaccuuggag gacagacgug uauuucaauc accagaccaa gaaguacgag     2940

cuuaugggac ugaaauauag cgauuugucg uuugaaaagg guuccggcaa auacucuauc     3000

agcaacgaga aguauaauuc aauuaaacgu auagaagggg ucaacgagca gagugaguuc     3060

aaguuuacac ucuacaagaa ugaccugauc cuaauuaaag auuccgaaaa caaugagcaa     3120

aagcuguucc gcuuuaacuc ucggaacgac acugcuaaac auuacguuga acucaagccc     3180

uaugauaaag ccagauucga cggacagcag gagcugauca ccauucuugg caauguggca     3240

aagggugggc aaugcuugaa aggccugaac aagagcaaua ucuccauauu caaggugaaa     3300

acggauguau uaggaaagaa acacaucauu aagaaagagg gcgacgaacc aaagcucaag     3360

uuuuga                                                                3366


<210>  2782
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2782
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu ugguaaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggauug ugcgccugaa uagacuauuu gaggaaucag gccucaucac ggacuucacc      240

aagauaagua ucaaccugaa uccuuaccag cuuaggguca aaggucugac agaugaguug      300

agcaacgaag aacucuuuau ugcccugaag aauaugguga agcaccgggg gaucuccuau      360

cuggacgaug cgucugacga uggaaacucg ucaguuggcg acuacgcuca gauugugaaa      420

gagaauagca agcagcucga aacuaaaacc ccaggccaaa uccaguuaga gcgcuaucag      480

acauacgggc agcugcgagg agauuucacc gucgaaaagg acgguaaaaa gcauagacuu      540

auaaacgugu uuccgacaag ugccuauagg uccgaggcau ugcggauucu gaagacucaa      600

caggaguuca auccccagau caccgaugaa uuuauuaacc guuaccuaga gauccucacg      660

ggcaaacgca aguauuacca cgggccugga aaugaaaaau cuagaacuga cuacggcagg      720

uaucggacca acggugagac acuggauaac aucuucggga uucuuauagg aaagugcacu      780

uuuuacccag aagaguuccg agccgcuaaa gcaagcuaua ccgcccagga guuuaaucug      840

uugaacgacc ucaauaaccu gacaguaccc accgaaacga agaaauuauc caaggagcaa      900

aagaaucaga ucauuaacua cguuaaaaau gaaaaggcua ugggcccugc caaacuguuc      960

aaguauaucg cgaaacuccu uucaugugac guggcagaua uaaagggcua ccgcauugac     1020

aagaguggga aagccgagau ccauacuuuu gaggcuuaua gaaagaugaa aacacuggag     1080

accuuggaua ucgagcagau ggacagggaa acucuagaua agcuggccua cguccucaca     1140

cugaacaccg agcgggaagg gauucaagag gcacuugaac acgaguucgc ugacgguagc     1200

uucucucaga aacaggugga ugagcuggug caguuucgca aggccaauuc cucaaucuuc     1260

ggcaaggggu ggcacaacuu uagcgucaaa uugaugaugg aacucauucc agagcuguac     1320

gaaacgagug aggagcaaau gaccauauua acaagacugg gaaagcagaa aacuaccucu     1380

uccagcaaua agacaaaaua uaucgacgaa aagcuacuca cugaggaaau uuacaacccc     1440

guuguggcga agucgguaag gcaggccauc aaaaucguga augcagcuau uaaggaguau     1500

ggcgauuucg acaacauagu caucgaaaug gcccgugaga ccaaugagga ugacgagaaa     1560

aaggcuauuc agaaaaucca aaaggccaac aaagacgaaa aggaugcagc caugcugaag     1620

gcugcgaacc aguacaaugg uaaagcagaa cuuccucauu cuguguuuca cgggcauaag     1680

caguuggcca caaaaauucg gcuguggcac cagcaaggcg agcgaugccu cuauaccgga     1740

aagacuaucu ccauacacga ccugaucaac aauccaaacc aguucgaagu ugaucauauu     1800

cuuccgcugu caaucacguu ugacgauagu uuggcuaaua aagugcucgu cuacgccaca     1860

gcaaaccagg agaagggcca gagaaccccc uaucaagccc uggacagcau ggaugacgcu     1920

ugguccuuca gggaguuaaa ggccuuugug cgcgaaucua aaacucugag caauaagaaa     1980

aaggaguacc uacucaccga agaggauauu ucaaaauucg acguacagaa gaaguuuauc     2040

gaacggaacc ugguugauac aagauacgca aguagggugg uccuuaaugc guugcaggag     2100

cacuuccgcg cucauaaaau agacaccaag guguccgucg ugcgagggca auuuacuagc     2160

cagcugcggc gucacugggg aauugagaaa acaagagaua cguaucauca ccacgccguu     2220

gacgcacuca ucauugccgc uucuucacag cugaacuuau ggaagaaaca gaagaauacc     2280

cuggugaguu acagcgaaga ccaacuuuug gauaucgaga cuggugaacu cauauccgac     2340

gaugaguaua aggagucugu auucaaagcc ccuuaccagc auuucgucga cacacugaag     2400

ucgaaagaau uugaggauag cauccuauuc uccuaucagg uggacucaaa guuuaacagg     2460

aaaauuagug augcaaccau cuacgcuaca cgccaggcca agguuggcaa agacaagaag     2520

gaugaaacuu augugcuggg gaaaauuaag gacaucuaca cccaagaugg auacgacgcg     2580

uucaugaaaa uauauaagaa agauaagucu aaguuucuca uguaccggca cgacccacag     2640

acguucgaga aagucauuga acccauccug gagaauuauc cuaacaagga gaugaaugaa     2700

aaaggcaagg aggugccaug uaaccccuuu cuuaaauaca aggaggaaca ugguuauauc     2760

agaaaguaca gcaaaaaggg gaacggcccg gaaauuaaau ccuugaagua uuacgacuca     2820

aaacugggaa aucacaucga uauuaccccu aagaacagca auaacaaggu aguguuacag     2880

ucuguuuccc ccuggagggc cgacgucuac uucaauaaaa caacuggcaa guaugagaua     2940

cucgggcuga aauacgcuga ucuucaguuu gagaagggaa ccgguacaua uaaaaucagu     3000

caagaaaagu acaacgacau uaagaaaaag gagggcgugg auagcgacuc ugaguucaaa     3060

uuuacucugu auaagaauga uuugcuccug gugaaagaca ccgaaacgaa ggaacagcag     3120

cuauuccgau uucugucccg cacaaugccu aaaaagaagc acuacgucga gcucaaacca     3180

uaugauaagc aaaaauucga gggaggagaa gcacugauca agguucuugg caacguggcc     3240

aauucagguc agugcauuaa aggguuggcu aagaguaaca ucagcauaua caaggugcgg     3300

accgacguac ugggcaauca gcauaucauu aaaaacgagg gagauaagcc caaauuagac     3360

uucuga                                                                3366


<210>  2783
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2783
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu ugguaaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggcgcg ugagacugaa uaggcuauuu gaggaaucag gccucauuac ggacuucacc      240

aagaucagua uaaaccugaa uccuuaccag cuucggguca aaggucugac agaugaguug      300

agcaacgaag aacucuuuau cgcccugaag aauaugguga agcaccgcgg gauuuccuau      360

cuggacgaug cgucugacga uggaaacucg ucaaucggcg acuacgcuca gauuguuaaa      420

gagaauagca agcagcucga aacuaaaacc ccaggccaaa uccaguuaga gcgauaucag      480

acauacgggc agcugagagg agauuucacc guggaaaagg acgguaaaaa gcauaggcuu      540

auaaacgucu uuccgacuag cgccuaucgg uccgaggcau ugcguauucu gcaaacacag      600

caggaguuca auccccagau caccgaugaa uuuauuaacc gcuaccuaga gauccucacg      660

ggcaagagaa aauauuacca cgggccugga aaugaaaagu cuaggacuga cuacggccgg      720

uaucgaacca gcggugagac acuggauaac aucuucggga uucuuauagg aaaaugcacu      780

uuuuacccag acgaguuccg cgccgcuaag gcauccuaua ccgcccaaga guuuaaccug      840

uugaaugacc ucaacaaucu gacagugccc accgagacga aaaaguuauc aaaagaacag      900

aagaaccaga ucauuaauua cguaaagaac gagaaagcua ugggcccugc caagcuguuc      960

aaauauaucg cgaagcuccu uaguugugau guugcagaca uaaaaggcua cagaauugau     1020

aagagcggga aggccgaaau ccauacuuuu gaggcuuaua ggaaaaugaa gacacuggaa     1080

accuuggaca ucgagcaaau ggaucgggag acucuagaca aacuggccua cgugcucaca     1140

cugaauaccg aacgcgaggg uauucaggaa gcacuugagc acgaguucgc ugauggaucu     1200

uucucccaga agcaggucga cgaacuggug caauuuagaa aagccaacuc aagcaucuuc     1260

ggcaaggggu ggcacaauuu uagugugaag uugaugaugg agcucauucc agaacuguac     1320

gagacgucug aggaacagau gaccauauua acaaggcugg gaaaacagaa gacuaccucc     1380

agcucgaaca aaacaaagua uaucgaugag aaacuacuca cugaagagau uuacaauccc     1440

gucguugcga agucugugcg ucaggccauc aagaucguaa acgcagcuau uaaagaauau     1500

ggcgacuucg auaauauagu gaucgagaug gcccgggaaa ccaacgagga cgacgagaag     1560

aaagcuauuc aaaagaucca gaaagccaau aaggaugaaa aagacgcagc caugcugaag     1620

gcugcgaacc aguacaacgg uaaggcagag cuuccucauu ccgucuuuca cgggcauaaa     1680

caguuggcca caaagauucg acuguggcac caacagggcg aaagaugccu cuauaccgga     1740

aaaacuaucu caauacacga ucugaucaau aacaguaauc aguucgaggu ggaccauauu     1800

cuuccacuga gcaucacguu ugaugacucc uuggcuaaca agguucucgu guacgccaca     1860

gcaaaucagg aaaaaggcca aaggaccccg uaucaggccc uggauucuau ggacgaugcu     1920

uggagcuucc gcgaguuaaa ggccuuuguc cgggagucaa agacucugag uaacaaaaag     1980

aaagaauacc uacucaccga ggaagacauu uccaaguucg augugaggaa aaaguuuauc     2040

gagagaaauc ugguagacac acgcuacgca agccgaguug ugcuuaacgc guugcaggaa     2100

cacuuccggg cucauaagau agauaccaaa gucucugugg uccgugggca auuuacuuca     2160

cagcugagaa ggcacugggg aauugagaag acacgcgaca cguaucauca ccacgccgug     2220

gacgcacuca ucauugccgc uaguagccag cugaauuuau ggaaaaagca gaaaaacacc     2280

cugguuuccu acucugagga ucaacuuuug gacaucgaaa cuggugagcu cauaucggau     2340

gacgaauaua aggagagcgu guucaaggcc cccuaccagc auuucguaga uacacugaaa     2400

uccaaggagu uugaagacuc aauccuauuc aguuaucagg ucgauucuaa auuuaaucgg     2460

aagauuagcg acgcaaccau cuacgcuaca agacaggcca aagugggcaa ggauaaagcg     2520

gacgagacuu auguucuggg gaagauuaag gauaucuaca cccaagacgg auacgaugcc     2580

uucaugaaaa uauauaagaa agacaagucc aaauuucuca uguacaggca cgacccucag     2640

acguucgaaa aggugauuga gccaauccug gaaaacuauc ccaauaagca gaucaacgag     2700

aaaggcaagg aggucccuug uaauccauuu cuuaaauaca aggaggaaca ugguuauauu     2760

cgaaaauacu caaagaaggg gaacggcccc gaaaucaaaa guuugaagua uuacgauucu     2820

aaacugggaa accacauuga cauaaccccg aaggauucca auaacaaagu gguauuacag     2880

agugugagcc cuuggcgcgc ugacguuuac uucaauaaga caacuggcaa guaugagauc     2940

cucgggcuga aauacgcaga ucuucaauuu gaaaagggaa ccgguacaua uaaaauuucu     3000

caggagaagu acaacgacau caaaaagaag gagggcgucg auuccgacuc agaguucaaa     3060

uuuacucugu auaagaauga uuugcuccug gugaaagaca ccgaaacgaa ggaacagcaa     3120

cuauuccggu uucugaguag aacaaugccc aaacagaagc acuacgugga gcucaaacca     3180

uaugauaagc agaaguucga agggggagag gcccugauua aaguccuugg caacguugcu     3240

aauagcgguc agugcaagaa aggguugggc aagucgaaca ucuccauaua caaagugagg     3300

accgacgugc ugggaaauca acauaucauu aagaacgagg gcgacaagcc uaaauuagau     3360

uucuga                                                                3366


<210>  2784
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2784
augagcgacc uggugcucgg ccuugauaua gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu ugguaaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggcgcg ugagacugaa uaggcuauuu gaggaaucag gccucauuac ggacuucacc      240

aagaucagua uaaaccugaa uccuuaccag cuucggguca aaggucugac agaugaguug      300

agcaacgaag aacucuuuau cgcccugaag aauaugguga agcaccgcgg gauuuccuau      360

cuggacgaug cgucugacga uggaaacucg ucaguuggcg acuacgcuca gaucgugaaa      420

gagaauagca agcagcucga aacuaaaacc ccaggccaaa uucaguuaga gcgauaucag      480

acauacgggc agcugagagg agauuucacc gucgaaaagg acgguaaaaa gcauaggcuu      540

aucaacgugu uuccgacaag ugccuaucgg uccgaggcau ugcguauacu gcaaacucag      600

caggaguuca auccccagau uaccgaugaa uuuguaaacc gcuaccuaga gauccucacg      660

ggcaagagaa aauauuacca cgggccugga aaugaaaagu cuaggacuga cuacggccgg      720

uaucgaacca acggugagac acuggauaac auuuucggga uccuuaucgg aaaaugcacu      780

uuuuacccag aagaguuccg cgccgcuaag gcaagcuaua ccgcccaaga guuuaaucug      840

uugaacgacc ucaauaaccu gacaguuccc accgaaacga aaaaguuauc caaagagcag      900

aagaaucaga uuauaaacua cgugaagaau gaaaaagcua ugggcccugc caagcuguuc      960

aaauauaucg cgaagcuccu uucaugugac gucgcagaua uuaaaggcua cagaaucgac     1020

aagaguggga aggccgagau acauacuuuu gaggcuuaua ggaaaaugaa gacacuggag     1080

accuuggaua uugagcaaau ggaccgggaa acucuagaua aacuggccua cgugcucaca     1140

cugaacaccg agcgcgaagg aauccaggag gcacuugaac acgaguucgc ugacgguagc     1200

uucucucaga aacaggugga ugagcugguc caauuuagaa aagccaauuc cucaaucuuc     1260

ggcaaggggu ggcacaacuu uagcguuaag uugaugaugg aacucauucc agagcuguac     1320

gaaacgagug aggagcagau gaccaucuua aaaaggcugg gaaagcagaa aacaacuucu     1380

uccagcaaua agaccaaaua uauugacgaa aagcuacuca cagaggaaau auacaacccc     1440

gugguagcga agucgauccg ucaggccauu aaaaucguga augcagcuau caaggaguau     1500

ggcgauuucg acaacauugu cauagaaaug gcccgggaga cuaaugagga ugacgaaaag     1560

aaggcuaucc agaaaauuca aaaggccaac aaagacgaga aggaugcagc caugcugaag     1620

gcugcgaacc aguacaaugg uaaagcagaa cuuccucauu cuguguuuca cgggcauaag     1680

caguuggcca ccaaaauccg acuguggcac caacagggcg agagaugccu cuauacagga     1740

aagaccauuu ccauccacga ccugauaaac aauccaaacc aguucgaagu ugaucauauc     1800

cuuccgcugu caauuacuuu ugacgauagu uuggcuaaua aagugcucgu cuacgccacg     1860

gcaaaccagg agaagggcca aaggacaccc uaucaggccc uggacagcau ggaugacgcu     1920

ugguccuucc gcgaguuaaa ggccuuugug cgggaaucua aaacccugag caauaagaaa     1980

aaggaguacc uacucacuga agaggauauc ucaaaauucg acguaagaaa gaaguuuauu     2040

gaaaggaacc ugguugauac ccgcuacgca agucgagugg uccuuaaugc guugcaggag     2100

cacuuccggg cucauaaaau cgacacaaag guguccgucg ugcgugggca auuuaccagc     2160

cagcugagaa ggcacugggg aauagagaaa acucgcgaua cauaucauca ccacgccguu     2220

gacgcacuca uuaucgccgc uucuucacag cugaacuuau ggaagaaaca gaagaauacg     2280

cuggugaguu acagcgaaga ccaacuuuug gauauugaga ccggugaacu caucuccgac     2340

gaugaguaua aggagucugu auucaaagcc ccuuaccagc auuucgucga cacucugaag     2400

ucgaaagaau uugaggauag cauacuauuc uccuaucagg uggacucaaa guuuaaccgg     2460

aaaaucagug augcaacaau uuacgcuacc agacaggcca agguuggcaa agacaagaag     2520

gaugaaacau augugcuggg gaaaaucaag gacauuuaca cucaagaugg auacgacgcg     2580

uucaugaaaa ucuauaagaa agauaagucu aaguuucuca uguacaggca cgacccacag     2640

accuucgaga aagucauaga acccauucug gagaauuauc cuaacaagga gaugaaugaa     2700

aaaggcaagg aggugccaug uaaccccuuu cuuaaauaca aggaggaaca ugguuauauc     2760

cgaaaguaca gcaaaaaggg gaacgacccg gaaaucaaau ccuugaagua uuacgauuca     2820

aaacugggca aucacauuga caucacgccu aaggauagca acaauaaggu aguguuacag     2880

ucuguuuccc ccuggcgcgc cgacgucuac uucaacaaaa ccacaggaaa guaugagauu     2940

cucggccuga aauacgcuga ucuucaguuu gagaagggga cuggaaccua uaaaauaagu     3000

caagaaaagu acaaugacau caagaaaaag gagggugugg auagcgacuc ugaguucaaa     3060

uuuacacugu auaagaacga uuugcuccug gugaaagaca cugaaaccaa ggaacagcag     3120

cuauuccggu uucuguccag aacgaugcca aaacaaaagc acuacgucga gcucaagccu     3180

uaugauaaac agaaguucga gggcggggaa gcacugauua aaguucuugg aaauguggcc     3240

aacucaggcc agugcaagaa agguuugggg aagaguaaua ucagcauuua caaggugagg     3300

acagacguac ugggcaacca gcauaucauc aaaaaugagg gagacaagcc caaauuagau     3360

uucuga                                                                3366


<210>  2785
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2785
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaaaauu ccgggaaaau cauucacgcu aacucuagau uguuccccgc agccaccgcg      120

gauaauaacg uggaaaggcg gaagaaucgc caggcucgac guuuacauag aaggaaaaag      180

caccggggag uacgcuuuca agaccuguuc gaggauuacg gccuacucac agacuuuuca      240

aaagugagua ucaaccugaa uccuuauaga cuuagggucg auggucugaa ccagcaguug      300

acuaaugaag aacucuucau ugcccugaag aacaucguga agcggcgcgg gauaagcuac      360

cuggacgaug cauccgaaga cggaggcacg guuucuucgg auuauggcaa agccguggag      420

gagaaucgaa agcucuuagc ugaacagacc ccagggcaaa uccagcugga gagauuugaa      480

aaauacggac aggucagggg ugacuucaca gugguagaga acggcgagaa gcggcgucuu      540

auuaauguuu ucucaacuag cgcauaucgc aaagaagccg agagaaucuu gaggaagcag      600

caagaauuua acaguaagau uaccgaugag uuuaucgaag acugccugaa aauacuaaca      660

gggaagcgga aauacuauca uggaccgggc aaugagaagu cccgaaccga uuacggucgc      720

uucagaacug acgggacaac ccucgacaac auuuuuggaa uccugauugg caaauguacg      780

uucuaccccg augaguauag ggcuucuaag gccagcuaca cugcgcagga auuuaaccuu      840

cugaaugacu ugaacaaucu caccgugccu acagagacua aaaagcuguc cgaggagcag      900

aagaaaguca ucguggaaua ugcaaaggag gccaaaaccu uaggcgcuuc aacacugcuc      960

aaguacaucg ccaaaaugau ugaugcaagu guggaccaga uacgggggua ucgcgucgau     1020

guuaacaaua agccagagau ccacaccuuc gaggcuuaca gaaagaugca aagccuugaa     1080

acgauuucug ugggagaacu guccaggaac guauuggacg agcuagccca uauccugacu     1140

cucaauacag aacgugaggg uauagaggaa gcgauuaaca ccaaacugaa ggauucauuc     1200

agccaggacc aggugcuuga gcugguccaa uuucggaaaa auaacaguuc uuuguucucc     1260

aagggcuggc acaauuuuag ccucaaacug augauggaau uaauccccga gcuguaugag     1320

acuucggagg aacagaugac aauccuaacc cgacucggga agcagaaguc uaaagaaauu     1380

uccaagagaa cgaaauacau cgaugagaag gaacugaccg aggagauuua caacccugug     1440

guugccaaau cagugaggca ggcaauaaag aucauuaaug aagcuacaaa gaaacacgga     1500

aucuucgaca acaucgucau ugagauggcc cgcgaaaaua acgaggaaga ugcuaagaaa     1560

gacuauauaa agcggcaaaa agccaaccag gacgagaaga augcagccau ggagaaagcu     1620

gcguuucagu acaacggcaa gaaggaacuu ccagauagua ucuuccaugg ucagaaagag     1680

uuggcaacua agauuagacu guggcaccaa cagggggaaa aaugccucua uaccggcaag     1740

aauaucccca uuagcgaccu gauccauaac caguacaaau augagauaga ucacauccuu     1800

ccucuguccu ugucuuuuga cgauagccuc ucaaauaagg ugcugguauu agccacagcu     1860

aaccaggaaa agggacaaag gacuccauuc caggcccugg acaguaugga ugacgcaugg     1920

uccuaccgcg aguuuaaaag cuauguuaag gagucuaaac uacucggcaa uaagaaaaag     1980

gaauaccugc uuaccgagga agauauuuca aagaucgagg ugaaacagaa guucauugag     2040

cgaaacuuga ucgacacacg guacaguagc cgugucgugc ugaaugcccu ccaggauuuu     2100

uauaaagcuc acaaguucga caccacuaua uccgucguga gagggcaauu uacgucucag     2160

cugaggcgca aauggggauu agaaaagucg cgggagacau accaucacca ugccguugau     2220

gcacugauua ucgcggcuag cucccagcuu agauugugga agaaacaaaa caauccgcuc     2280

auugccuaua aggaagguca guucguggac ucagagaccg gcgaaaucau aagucugucu     2340

gacgaugagu acaaagagcu aguauucaag gcacccuaug accacuuugu cgauacucug     2400

agcuccaaaa ccuucgaaga cucaauccuc uuuagcuacc agguggauuc uaaguucaac     2460

aggaaaauuu ccgacgccac aaucuaugcu acccgaaagg ccaagcugga uaaagagaag     2520

agugaagaga cuuacguucu ugggaaaauu aaggacaucu acagccaggc aggauaugau     2580

gcuuuuauaa aaauuuacaa uaaggacaag ucuaaauucu ugauguauca uaaggauccu     2640

caaacauuug aaaaagugau cgaggagauc cugcgcacgu acccauccaa ggaauuaaac     2700

gacaaaaaua aggagauucc cuguaacccu uucgaaaagu aucggcagga gaacggccca     2760

aucagaaaau acucaaagaa aaguaauggu cccgaaauua agagccucaa auauuacgau     2820

aacaagcugg ggaaucacau agacaucacc ccggagucgu ccgacaacca ggucgugcuu     2880

cagucucuga agccuuggag gacugaugua uacuuuaauc accaaacaaa gaaguaugag     2940

uugaugggcc ucaaauacag cgaccuguca uucgaaaagg gaaguggcaa auauuccauu     3000

ucuaaccaga aguacaauag caucaagcgu auugagggga ucgaugaaca guccgaguuu     3060

aaauucaccc uauauaagaa cgaccugaua cucaucaaag auucagagaa uaacgaacag     3120

aagcuguuuc gcuucaauag ccggaacgac acagccaaac auuacauuga gcuuaagccc     3180

uaugauaaag cgaaguucga cggagaacaa gaguugauca cuauucuggg uaauguggcc     3240

aagggcggga gaugcuuaaa aggacucaac aagaguaaua ucucuauauu uaaaguuaag     3300

accgaugucc ugggcaaaaa gcacauuauc aagaaagaag gugacgagcc aaaacuuaag     3360

uucuga                                                                3366


<210>  2786
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2786
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

aauaaggaua ccggggagau cauucacaca aacuccagaa ucuuccccgc ugcaacugcc      120

gacaauaacg uggaaaggcg caauucucgg caggaucgac guuugaacag aaggaaaaag      180

caucggggag uacgcuuaca cgaccuguuu gaggauuacg accuacucac ggauuucuca      240

aaagugagua uuaaucugaa cccuuauaga cuuagggucc aaggccugga caauagcuug      300

accacagaag aacucuuuau cgcgcugaag aacauaguga aacggcgcgg uaucuccuac      360

cuggaugacg cuucugaaga ugggggaguu gugucgucag acuauggcaa ggccgucgag      420

gagaaucgaa agcucuuagc agaacagacu ccaggccaga uucagcugga gagauucaaa      480

aaguacgggc aagugagggg agauuuuacc guaguugaaa acggugagaa acggcgucuu      540

aucaaugugu ucagcacaag ugccuaucgc aaggaggcug aaagaauuuu gaggaaacag      600

caggaguuua acaaucagau caccgacaag uucauagaag acuaccugaa gauucuaacu      660

gagaaacgga aguauuacca uggcccgggg aacgaaaaau cccgaacaga uuacggacgc      720

uuuagaaccg acggcacgac ucucgauaac aucuucggua uucugaucgg gaagugcacc      780

uuuuauccca augaguacag ggcaucuaaa gccagcuaua cagcucaaga guucaaccuu      840

cugaaugacu ugaacaaucu cacugucccu accgaaacaa agaaacuguc cgaggaacag      900

aagaagacca ucguggagua cgccaaauca gcgaagacgu uaggagcaag uacucugcuc      960

aaauauauug ccaagaugau agaugcuagc guggaccaga uccggggcua ccgcgucgau     1020

guuaacaaua aaccagaaau gcacacauuu gagguguaua gaaagaugca gucucuugag     1080

accauuuccg uaugggaacu gucaaggaac aucuuggacg agcuagccca uauacugacu     1140

cucaauacag aacgugaggg cauugaagag gcaaucaaca ccaagcugau ggauguguuc     1200

agccaagacc agguccuuga gcuggugcag uuccggaaaa auaacaguuc uuuguuuucc     1260

aagggguggc acaauuucag ccgaaaacuc augauggaac ugauccccga guuauacgaa     1320

acgucggagg agcaaaugac cauucugaca agacuaggaa agcagaaauc uaaggaaacu     1380

uccaagagga ccaaauacau cgaugagaag gaaguuacag aggaaauuua uaacccugug     1440

gucgcuaaau cagugcgcca ggccauaaag aucauuaaug aggcgacuaa aaaguacggu     1500

aucuuugaca acaucguaau ugagauggcc cgggaaaaca augaggaaga cgcaaagaaa     1560

gauuauauaa agagacagaa agcuaaccaa gacgagaaga auguugccau cgaaaaagcu     1620

gccuuccagu acaacggcaa gaaagagcuc ccagauagua uuuuucacgg gcauaaggag     1680

cuggcaacca agaucaggcu uuggcaccag cagggagaaa aauguuugua uacaggcaag     1740

aauauuccca ucagcgaccu gauacauaac caauacaaau augagaucga ucacauucuc     1800

ccucuguccc uuucuuucga cgauagccug ucaaauaagg uguugguccu cgccaccgcu     1860

aaccaggaaa aaggucagcg cacuccauuu caggcgcugg acaguaugga ugacgcaugg     1920

uccuaccgag aguucaagag cuacgugaag gauucuaaau uacuggggaa uaagaaaaag     1980

gacuaucuac ucacggaaga ggauaucuca aaaauugagg ucaagcaaaa guuuaucgaa     2040

cggaaccugg uggacacacg uuacaguagc agaguugugc uuaauaccuu gcaggacuuc     2100

uauaaagccc acaaguuuga uacuaccaua uccguaguca ggggccaguu cacaucucag     2160

cugcgccgga aauggggacu cgagaagucg agagaaaccu accaucacca ugcuguggac     2220

gcccugauua ucgcagccag cucccaauua aggcugugga aaaagcaggc uaacccgcuu     2280

auuucauaua aggaggauca guuuguugac agugagacug gcgaaaucau aucuuugaca     2340

gaugacaaau acaaggagcu cguguucaaa gcccccuaug cacacuucgu cgauacgcug     2400

agcuccaaga aauuugaaga cucaauccua uucagcuacc aaguggauuc uaaguacaau     2460

cgaaaaauuu ccgacgcgac caucuaugcu acucgcaagg uaaagcugga uaaagacaag     2520

agugaggaaa cauacgugcu cgggaaaauu aaggauaucu auagccaggc cggauacgac     2580

gcauuuauaa aaauuuauaa caaggacaag ucuaaauucc ugauguacca uaaggauccu     2640

cagaccuuug agaaaguuau cgaggaaauc cuucggauuu auccauccaa ggaguucaau     2700

gagaaaaaca aggagguccc cugcaacccu uuugaaaagu acagacagga gaauggucca     2760

aucuguaaau acucaaagaa aggcaacggg cccgagauua agaguuugaa auauuacgac     2820

aagaauauac cggaaaguuu caucgauauu acaccuucga acuccaagaa uaaagugauc     2880

cugaggcaau uaaaccccug gcguacugac guguauuuua aucaccagac cgagaaguac     2940

gaacuccugg gacuuaaaua ugccgaucug caguucgaga agaaaacgga aaccuacucu     3000

auuacaaucg acaaguauaa cgagauaaag gagaaagaag gcgucgguga gcacagcgaa     3060

uuuaaguuca cuuuguacaa aaaugaucuc cugcuaauca aggacaugga gauuaaagag     3120

gaacagaucu uucgcuuccu gucacggacc augccaaaca agaaacauua cguugagcuc     3180

aagccuuauu acaaggggaa uuucgaaggc aaagagagaa uuaagguguu uaacacaacu     3240

agcaauggaa ggugccugaa aggccuuggg aagaaaggau uguccaucua uaagauaaag     3300

accgaugugc uggguaacaa acacuucauu aagaaagaag gcgagaagcc caaauuaaag     3360

uuuuga                                                                3366


<210>  2787
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2787
auggacagaa ccgaggugaa caucggcuuc gauauuggga uaagcuccgu cggauggucu       60

guuguggcca caaagucagg uaggauccug gaaaguggcg uaagccucuu uuccggggcu      120

ucugcagcca aaaaugagga acggcgcaac uuccgacagu cgcguagacu ucugaggcgg      180

cgcaagaaua gaauuucaga cuugaaaaag uacuuaaaag agcagagcuu uacuuauaac      240

ggagaacugc uaaaugagaa ccccuacgag aucaggguga agggccucac ggaaaagcug      300

aguaaagagg aaauugcgcu ugcucuguug cacaucguca agcggcgcgg uguguccuau      360

gaucucgggg acauagagga ggaaggaacc acaggcucuu acaaagaggg cguuaagaaa      420

aaucaaaagc ugcugaagga aaagacuccu gccgagaucc aguuccagcg acucaagaaa      480

uuucauaagg ugagagggca aauugcaauc gaugaaaacg acgucuuacu gaauguguuc      540

ccaaaccacg ccuauguaga ugaggcucag cagauucuuu acacccagca agaguuuuau      600

ccggaaauca augagacauu cauaaaccag guuaccgacu uuauuagcag gaaacgggau      660

uacuucaucg gacccgguuc cgaaaaguca cguacugacu acggcauuua ucgcacagau      720

gggaccacgu ugcagaaucu guuuaguauc cuaaucggaa aagacaagau uuucccugau      780

gaguuuagag cagccggcaa cagcuacacu gcucaggugu auaaucuccu gaacgaccuu      840

aacaaucugg ccauagacga uaccgaaaca gguaaguuga cuaaacaaca gaaggagaaa      900

aucauuaugg agcucaagac cacagaaaaa ucugucaaca ugaugaagcu gaucgcgaag      960

accgcagggg ugacggugga gcagauaucc agguaccgga uuaaucgaga cggaaaauca     1020

gaaauccaua acauggccgu cuaucgcaag aucagaaaag uuuucuuaga gcugggcuac     1080

gaaauuaaug aguggccaag ccaacuccuu gaugagcugg gcgacgcuuu gagucuaaac     1140

acugaaaaug gggagaucag gcggauucug acagauaacc ucgccaauaa guauaaaaua     1200

cuggaccagc agcuuaucaa ccugauuauc gaaaauaaga acgcauucac cuugucuacu     1260

aaucagaagu ggcaccgcuu uuccuacaaa acaaugaaga uccucauucc cgagcuguua     1320

gagaccaaca aagaacaaau gacgauacug accgagaugg gacuaaugaa gaaagaucag     1380

agaaauuacg uggguaagaa caagaucgac agcgaagcuc uccuggagaa ccuuuauaau     1440

ccuguaguga ggaaaucgac acgucagacu augaaguugu ucaacacccu gcucaaaaag     1500

uacccaaaua uugccuaugu cgugaucgaa augccccggg aggaugacga ggauaaagcg     1560

cgaagagacu acguuaaguu ucagaaagac aacgaaaagg agaaggaugc cgcacugaaa     1620

aauuucauua uggaaucugg caaguccgag gcugaaguga ucgccaaaua uaggcaauca     1680

aagaaauuuc gcaguaaggu ccggcuuugg uaccagcaga uggggagaug ccuguauagc     1740

ggaaaggcua ugccugccga gaggguguuc cgcgacgauc auuuuacaga cauagaucac     1800

guaaucccau uguccguuuc uuucgacgau ggcaugaaca auaaagugcu cugucuggca     1860

gagaugaacc gacagaagga caaacaaacu ccguacgccu uuuucaaugc uggucacggg     1920

cagggcuuuc aggcgaugaa cgcaauguua aagaauaaca aucggauuag cucagccaaa     1980

aagcguaacc ugcuauucac cgaaaaucuc aacgaucugg aggucagaaa gagguuuauc     2040

gaacgcaacc uuguggacac acgguacgcu aguagagucg uguugaauga gcugcagcaa     2100

uucguuaaag ccaagcagcu cgauaccaaa gugacuguaa uuaggggaaa guucacgucc     2160

aaacugcgag aaaaguggaa caucaauaag agccgcgaca cacaucacca ucacgcaguc     2220

gaugccgcug ugauagccgu uucucccaug uuaaaccugu gggagaaagg cgcaucaauu     2280

aucccuaaua agguggggga gaacgucauu gacaucaaaa ccggagaaau aaucacugac     2340

cacgauuaug aggcggacgu guacgaacuu ccauaugcua aguuuuugga gcagauuccc     2400

aaaguaaaga aagauaucaa guuccggcau caaguggaca agaaaaagaa uagaaaaguu     2460

agugaugcca ccauuuacac aaccaggaag gcaaaagucg guaaggacaa ggaggaaacu     2520

gauuaugugc ucggcacaau caaagacaua uacagccugg agggguaugu guccuuuaag     2580

aaaauuuaca acaaggauaa agccaaguuc cuaauggcuc guaucgaccc uaagacguuu     2640

gaaaaacugg agaagaucgu cgccgaauac ccagagucua uugagguugu gcaggauaau     2700

ggaaaagugg acguacagaa gaucucgccc uucgaacucu aucgcaaaga uaacggcuuu     2760

gucaccaagu ucgcaaagaa aaauaacggu ccggcuguua agagccugaa auacuaugac     2820

uccaaacuug ggucaaguau ugacgugacu ccugauaaug ccaaaaacaa gaaggugaua     2880

uugcagucuc ugaaacccug gcggacagac gucuacuuua augaggaaaa ccaagaguau     2940

gaaaucaugg gcuuaaagua cagcgaucuc aaauucggaa agaacggcaa auauggggug     3000

ccaaagguua gauacgagga gauuaaacag gaagaggaaa ucuccgaggg aucaaaguuu     3060

cuguucagcc uuuacagggg ugaccgaauu aaggugacca augaacuggg cgagaacuug     3120

gaucuccugu uuucuucccg gacacuaccu gcgcagaaag gguauguaga gcugaagccc     3180

aucgacaaaa cuaaguucga uggaaaagaa aaggucaguu ucuacggcac cgugacgcca     3240

aauggucagu uugugaagaa auucgccaag aaaggguaua agauacucaa acugaacacc     3300

gacgagcuug gcaauacaua caucgaaaac ggagagaagg agcccagcga uauuuuggac     3360

auguga                                                                3366


<210>  2788
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2788
augaacggcc uggugcucgg gcuugacauc ggaauuggua gcgucggcgu ugggauacug       60

aauaaggaua ccggagagau cauucacgcc aacuccagaa ucuuccccgc ugcaacagcc      120

gacaauaacg ugguaaggcg gucuucacgc cagggccgac guuugaauca uaggaaaaag      180

cacagaagug ugcgguuaaa agaucuguuu gaauacuaug accuacucac ugauuucagc      240

aaggucucca uuaaccugaa uccuuaccgc cuuagaguga acggucugaa ucaacaguug      300

acgaacgagg aacucuuuau cgcgcugaaa aauauaguua agaggcgggg gaucucuuau      360

cuggacgaug cuucggagga cggaggcgcc gugucaagcg auuacggcaa ggcagucgaa      420

gagaaccgca aacucuuagc cgagcagacc ccagggcaga uucaacugga acgauucgag      480

aaguauggac aggugagagg ugacuuuaca guaguugaaa auggcgagaa aaggcggcuu      540

aucaacgugu ucaguacuuc cgcuuacaag aaagaggcag aacguauuuu gcgcaagcag      600

caggaguaua auaaccaaau cauggaugaa uuuauagccg acuaccugac cauucuaaca      660

gggaagagaa aauacuauca uggaccgggc aacgagaagu cuaggaccga uuacggucgg      720

uuccgaacug acgggacaac ccucgacaau aucuuuggca uucugaucgg aaaaugcacg      780

uucuaucccg aaaaguaccg cgcuagcaaa gccuccuaua cugcgcagga guuuaaccuu      840

cugaaugauu ugaacaaucu caccgucccu acagagacua agaaacuguc agaagaacag      900

aagaagacca ucguggaaua cgcaaaaagu gccaagacau uaggcgcuag cacccugcuc      960

aaauauauug ccaagaugau agacgcaucu guggaucaga ucagagggua cagggucgac     1020

guuaaaaaca agccagagau gcacacguuc gaaguguacc ggaagaugca aucccuugag     1080

acuauuucag uaggagaguu uagccgcgaa gugcuggaug aguuggcuca uguccuaaca     1140

cugaauaccg aaagagaggg uaucgaagag gccauaaaca cuaaacucag ggacaguuuc     1200

ucucaggauc agauucugga gcuugugcaa uuccguaaga auaacuccag ccuguuuucg     1260

aaaggcuggc acaauuucuc uuugaagcuc augauggaac ugauccccga guuauaugaa     1320

acauccgagg agcagauggc gauccugacc cggcuaggga aacagaaguu uaaggaaacg     1380

ucaaaacgaa ccaaguacau ugacgagcag gaaguuacag aggaaaucua uaacccugug     1440

gucgccaaaa gugugagaca agcaauuaag auaaucaaug aggcuacuaa aaaguacgga     1500

auuuucgaua acaucguaau cgagauggcc agggaaaaua acgaggaaga ggcuaagaaa     1560

gacuauauua agcgccagaa agccaaccac gaugaaaaga augcagccau ggagaaagcu     1620

gcguuucagu acaacggcga gaaggaacuc ccagacagca uauuccaugg ucacaaagag     1680

cuggcaacca agguucggcu uuggcaucag caaggggaaa aguguuugua uacaggcaaa     1740

aauaucccca uuuccgaccu gauccacaac caguacaagu acgagauuga ucacauccuc     1800

ccucugucuc uuagcuuuga cgauucacug aguaauaaag uguugguccu cgccacugcu     1860

aaccaggaaa agggacagag aaccccauuc caagcccugg acuccaugga ugacgcaugg     1920

agcuauaggg aguuuaaauc uuacgugaag gauucaaaga guuuaggcaa uaaaaagaaa     1980

gacuaucugc uaacagagga agauauaagc aagaucgagg ucaaacagaa guucauugaa     2040

cgcaaccucg uggacacccg auacuccucu cggguugugc ugaauacucu ucaggauuuu     2100

uauaaggccc auaaauucga cacgacaauc ucgguagucc gugggcaguu uaccagccaa     2160

uugagaagga aguggggacu ggagaaaucc cgcgagacuu accaccauca cgcuguggau     2220

gcccucauua ucgcagcguc aagucagcug cgguuaugga agaaacagaa caauccgcug     2280

auaucuuaua aggaagguca auucguugac agcgagaccg gcgaaauuau cucccuuaca     2340

gacgaugagu acaaggaauu gguguucaaa gcucccuacg accauuuugu cgauacccuc     2400

agaucaaaga aauucgagga cagcauucug uuuucuuauc agguggauuc caaguucaac     2460

aggaaaauca gugacgccac uauauacgca acacgaaaga cgaaacuaga uaaggacaag     2520

agcgaggaaa ccuauguacu ggggaaaauc aaggauauuu acucucaguc cggauaugac     2580

gccuuuauca aaauuuacaa uaaggauaaa ucaaaguucc ucauguauca caaggacccu     2640

cagacuuuug agaaagugau cgaagagaua cugcgcacau acccaaguaa ggaguucaac     2700

gagaaaaaua aggagguuag cugcaacccc uuugaaaaau acaagcaaga gaacggcccu     2760

auucggaagu auucgaaaaa ggguaauggg ccagaaauca aaucccuuaa guacuaugau     2820

aaaaacaucc ccgagucuuu cauuaauauc accccgagca acucaaagaa uaaggucauu     2880

uugagacagc ugaacccuug gaggacagac guguacuuua aucaccagac ugaaaaauau     2940

gaguuacucg gccugaagua cgcugaccuu caguucgaga aaaagaccgg aacguauagu     3000

auaaccaucg auaaauacaa cgaaauuaag gagaaggaag gcguguccga gcauucugag     3060

uuuaaauuca cacuguacaa gaaugacuug cuccugauca aagauacuga aauuaaggag     3120

gaacaaaucu uccguuuucu aagccgcacc augcccaacc agaaacacua ugucgagcug     3180

aagccauacu auaaagggaa uuucgaagga aaggagcgga uaaagguuuu uaacacaacu     3240

uccaauggua gaugucucaa aggccugagg aagaaagggc uuucaaucua caagauuaaa     3300

accgacgugu ugggaaacaa gcauuucauc aagaaagagg gcgaaaagcc uaaacugaag     3360

uuuuga                                                                3366


<210>  2789
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2789
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu ugguaaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggauug ugcgccugaa uagacuauuu gaggaaucag gccucaucac ggacuucacc      240

aagauaagua ucaaccugaa uccuuaccag cuuaggguca aaggucugac agaugaguug      300

agcaacgaag aacucuuuau ugcccugaag aauaugguga agcaccgggg gaucuccuau      360

cuggacgaug cgucugacga uggaaacucg ucaguuggcg acuacgcuca gauugugaaa      420

gagaauagca agcagcucga aacuaaaacc ccaggccaaa uccaguuaga gcgcuaucag      480

acauacgggc agcugcgagg agauuucacc gucgaaaagg acgguaaaaa gcauagacuu      540

auaaacgugu uuccgacuag cgccuauagg uccgaggcau ugcggauucu gcaaacacag      600

caggaguuca auccccagau caccgaugaa uuuauuaacc guuaccuaga gauccucacg      660

ggcaagcgca aauauuacca cgggccugga aaugaaaagu cuagaacuga cuacggcagg      720

uaucggacca gcggugagac acuggauaac aucuucggga uucuuauagg aaaaugcacu      780

uuuuacccag acgaguuccg agccgcuaag gcauccuaua ccgcccaaga guuuaaccug      840

uugaaugacc ucaacaaucu gacaguaccc accgagacga aaaaguuauc aaaagaacag      900

aagaaccaga ucauuaauua cguuaagaac gagaaagcua ugggcccugc caagcuguuc      960

aaauauaucg cgaagcuccu uaguugugau guggcagaca uaaaagaaua ccgcauugau     1020

aagagcggca aggccgagau ccauacuuuu gaggcuuaua gaaaaaugaa gacacuggaa     1080

accuuggaca ucgagcaaau ggauagggaa acucuagaca aacuggccua cguccucaca     1140

cugaauaccg agcgggaagg gauucaggag gcacuugaac acgaguucgc ugauggaucu     1200

uucucccaga aacaggugga cgagcuggug caauuucgca aggccaacuc aagcaucuuc     1260

gguaagggcu ggcacaauuu uagugucaag uugaugaugg aacucauucc agagcuguac     1320

gaaacgucug aggagcagau gaccauauua acaagacugg ggaaacagaa gacuaccucc     1380

agcucgaaca aaacaaagua uaucgaugaa aaacuacuca cugaggaaau uuacaauccc     1440

guuguggcga agucuguaag gcaggccauc aagaucguga acgcagcuau uaaagaguau     1500

ggagacuucg auaauauagu caucgaaaug gcccgugaga ccaacgagga cgacgaaaag     1560

aaagcuauuc aaaagaucca gaaagccaau aaggaugaga aagacgcagc caugcugaag     1620

gcugcgaacc aguacaacgg caaggcagaa cuuccucauu ccguguuuca cggucauaaa     1680

caguuggcca caaagauucg gcuguggcac caacaggggg agcgaugccu cuauaccggc     1740

aaaacuaucu caauacacga ucugaucaau aacaguaauc aguucgaagu ugaccauauu     1800

cuuccacuga gcaucacguu ugaugacucc uuggcuaaca aggugcucgu cuacgccaca     1860

gcaaaucagg agaaaggaca aagaaccccg uaucaggccc uggauucuau ggacgaugcu     1920

uggagcuuca gggaguuaaa ggccuuugug cgcgaaucaa agacucugag uaacaaaaag     1980

aaagaguacc uacucaccga agaggacauu uccaaguucg auguacggaa aaaguuuauc     2040

gaaagaaauc ugguugacac aagguacgca agccgcgugg uccuuaacgc guugcaggag     2100

cacuuccgag cucauaagau agauaccaaa gugucugucg ugcggggcca auuuacuuca     2160

cagcugcgua gacacugggg gauugagaag acaagggaca cguaucauca ccacgccguu     2220

gacgcacuca ucauugccgc uaguauccag cugaauuuau ggaaaaagca gaaaaacacc     2280

cuggugagcu acuccgaaga ucaacuuuug gacauagaga cuggagaacu caucucugau     2340

gacgaguaua aggagucggu auucaaggcc cccuaccagc auuucgucga uacacugaaa     2400

agcaaggaau uugaggacuc cauucuauuc ucauaucagg uggauaguaa auuuaaucgc     2460

aagaucucug acgcaaccau uuacgcuaca cggcaggcca aaguugguaa ggauaaagcg     2520

gacgaaacuu augugcuggg caagaucaag gauauauaca cccaagacgg guacgaugcc     2580

uucaugaaaa uuuauaagaa agacaagagc aaauuucuca uguacagaca cgacccucag     2640

acguucgaga aggucaucga accaauccug gagaacuauc ccaauaagca gauuaacgag     2700

aaaggaaagg aagugccugu aaauccauuu cuuaaauaca aggaggaaca uggcuauauc     2760

aggaaauacu ccaagaaggg uaacgggccc gagauuaaau cauugaagua uuacgauagc     2820

aaacugggca accacauaga caucaccccg aaggauucua auaacaaagu gguuuuacag     2880

uccgucaguc cuuggcgagc ugacguguac uucaauaaga caacuggaaa guaugaaauu     2940

cucggccuga aauacgcaga ucuucaauuu gagaagggga ccggaacaua uaaaaucagc     3000

caggagaagu acaacgacau uaaaaagaag gaaggugugg auucugacuc cgaguucaaa     3060

uuuacucugu auaagaauga uuugcuccug gucaaagaca ccgaaacgaa ggagcagcaa     3120

cuauuccgcu uucugucacg gacaaugccc aaacagaagc acuacguuga acucaaacca     3180

uaugauaagc agaaguucga gggcggggag gcccugauca aagugcuugg aaacguggcu     3240

aauaguggcc aguguaagaa agguuugggg aagaguaaca uaucgaucua caaaguaaga     3300

accgacgucc ugggcaauca acauauuauc aagaacgaag gagacaagcc uaaauuagau     3360

uucuga                                                                3366


<210>  2790
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2790
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaaaauu ccgggaaaau cauucacgcu aacucuagau uguuccccgc agccaccgcg      120

gauaauaacg uggaaaggcg gaagaaucgc caggcucgac guuuaaacag aaggaaaaag      180

caucggggag uacgcuuuca agaccuguuc gaggauuacg gccuacucac agacuuuuca      240

aaagugagua ucaaucugaa cccuuauaga cuuagggucg auggucugaa ucagcaguug      300

acuaacgaag aacucuucau ugcccugaag aauaucguga agcggcgcgg gauaagcuac      360

cuggacgaug cauccgaaga cggaggcgcc guuucuucgg auuauggcaa agcuguggag      420

gagaaccgaa agcucuuagc agaacagacg ccagggcaaa uccagcugga gagauuugaa      480

aaauacggac aggucagggg ugacuucacc gugguagaga auggcgagaa gugccggcuu      540

auuaacguuu ucucaacaag cgccuaucgu aaagaagcug agaagaucuu gcgcaagcag      600

caagaauuua auaaccagau uacugaugag uuuaucgaag acuaccugaa aauacuaacc      660

gggaagagaa aauauuacca cggaccgggc aacgagaaga guaggacaga uuacggucgg      720

uuccgaaccg acgggacuac acucgacaau auuuuuggag ugcugaucgg caaauguacc      780

uucuauccca acgaguaccg cgccuccaag ucuagcuaua cggcgcagga auuuaaucuu      840

cugaacgauu ugaauaaccu cacugucccu accgagacaa agaagcuguc cgaggagcag      900

aagaaaguga uuguggaaua cgcaaaggag gccaaaacuu uaggcgcuuc aacccugcuc      960

aaguauaucg ucaaaaugau cgacgccagu guugaucaaa uuagcgggua cagaguggac     1020

guaaauaaca agccagagau gcauacauuc gaaguguaua ggaagaugca gucucuugag     1080

accauauccg ucggagaacu gucacggaau guguuggaug agcuagcaca cauccugacg     1140

cucaacacug aacgcgaggg uauugaggaa gcuaucaaua gcaaacugau gggcguuuuc     1200

agucaggacc aagugcuuga gcugguccag uuuagaaaga acaauucuuc cuuguucagc     1260

aaaggguggc auaacuuuuc gcucaagcug augauggaau uaauacccga gcuguacgag     1320

acaucugagg aacagaugac cauucuaacu aggcucggaa aacagaaguc caaggaaaca     1380

ucaaaacgua ccaaguacau cgaugagaaa gaacugacgg aggagaucua uaauccugug     1440

guagccaaga guguucggca agcgauuaaa aucauuaacg aagccaccaa gaagcacggc     1500

auauucgaca acaucgugau ugagauggca cgagaaaaua acgaggaaga ugcuaaaaag     1560

gacuacauca aaagacagaa ggccaaucag gaugagaaaa acgcugccau ggagaaggca     1620

gccuuucagu auaaugguaa aaaggaacuu ccagggaaca ucuucaaugg ccacaaggag     1680

uuggcuacaa aaauuaggcu guggcaucaa cagggagaaa agugccucua cacuggcaaa     1740

accauaccca ucagcgaccu gauucacaac caguauaagu acgagaucga ccauauucuu     1800

ccucuguccu ugucuuuuga ugacagccuc ucaaauaaag uccuggugca cgcgacagca     1860

aaccaggaaa aggggcaacg cacuccauuc caggccuuag auaguaugga cgaugcuugg     1920

uccuaucggg aguuuaagag cuacgucaaa gaccugaagu cucuaucaaa uaaaaagaaa     1980

gauuaccucc ugaccgagga agacaucagu aagauagagg ugaagcagaa auucaucgaa     2040

agaaaccuug uugauacaag guauagcucc cgcgugguau ugaaugcccu gcaggacuuu     2100

uacaagucuc accaauucga uaccacuauu ucggucgugc gaggacaguu uacgagccag     2160

cuccggcgua aauggggucu ggagaagucc agagagacau aucaucacca ugcaguugac     2220

gccuuaauca uugcugccuc aagucaacug aggcuuugga aaaagcagaa caauccguug     2280

aucaccuaca aggaaggcca guucguggau ucucagacug gggagauaau uagccucacc     2340

gacgacgaau auaaagagcu ggucuucaag gcacccuacg aucacuuugu ggacacacua     2400

cauuccaaaa ccuucgaaga uggaauccug uuuucauauc aaguagacag caaguacaac     2460

cgcaaaauuu cugaugcgac uaucuacgcu acacggaagg ccaaacucga caaggauaag     2520

uccgaggaga cguaugugcu gggcaaaaua aaggacaucu acagucaggc agguuaugau     2580

gccuucauua aaaucuacaa caaggacaaa agaaaguuuc uuauguauca caaggauccu     2640

cagaccuucg aaaaaguuau ugaggaaauc uugaggacuu acccaagcaa ggagcgaaau     2700

gacaaaaaca aggaaguccc cuguaauccu uuugagaaau aucgccagga gaacgggcca     2760

auacggaagu acucuaagaa aggcaaugga cccgaaauua agugccugaa auacuaugau     2820

aacaaguuag gcaauuacau cgacaucucc ccggacgggu cagauaacca aguggugcuc     2880

cagagucuga aaccuuggag aacagacguc uauuucaauc accagaccaa ggaguacgaa     2940

cuuaugggac ugaaguauag cgauuugucg uuugagaaag guuccggcaa guacucuauu     3000

agcaacgaaa aauauaauuc aaucaagagg auugaggggg uuaacgagca gagugaguuc     3060

aaauuuacac ucuacaagaa ugaccugaua cuaaucaagg auuccgaaaa caaugaacaa     3120

aaacuguucc guuuuaacuc ucgcaacgac acugcuaagc auuacgugga gcucaaaccc     3180

uaugauaagg cccgguucga cggacagcag gagcugauua ccauccuugg caauguggca     3240

aaaggugggc aauguuugaa gggccugaac aaaagcaaua uuuccaucuu caagguaaag     3300

acggaugucu uaggcaaaaa gcacauaauc aaaaaggaag gagacgagcc aaaacucaag     3360

uuuuga                                                                3366


<210>  2791
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2791
auggccgaca agacccuggg cgcucucaca uucggguuug auaucggaau ugcaagcgug       60

gguuggugcg uccuuggcga gaacagaaua guugaccugg gggugagggc cuucgauaaa      120

gcggaaacug cuaaggaggg agaauccuug aauuuagccc ggcgcacggc acgacugaug      180

cguagaaggc uacggcgcag agccuggagg cucaccaaac uggcucggcu ucugaagcgc      240

gagggcuuga ucgcagacgc cuuucagaug caccaacccu cugcuacauc accuuggaaa      300

cuccgaguag aagcccugga uagaaggcug acugacgaug agugggcgcg ggugauuuac      360

caucucugua agcaccgugg uuuccauugg aucagucgcg cagaggaaaa gaaagccgag      420

ggggacgcua agggagaaag cggcagaguc aaaaagggcu uauccgauac ccagaggcgg      480

augggggaga aaggauaucg aucugccgca gagauggugc uggcugaauu uccagaggcc      540

cagcgcaaca agcaggguga cuacggcaag gcgcuuucga gaguuuugcu gucagaagaa      600

cuagcccucc uguucaggcg gcaacgcgaa cuugggcaca cacaugcaag caaagagcug      660

gaggcugcca uuuugggaaa uggcgauaga aagggugggc ucuuuugggc ucagaaaccg      720

ccccuggccg gagcagacuu acugaagaug cucggcaaau gcaccuucga aaaggaugag      780

uauagggccc cuaaagcuag uuuuacugcg gaacgucacg uguggcuuac acggcugaac      840

aauuugcgag ucgugguaga cggcaccacg agaccacuaa cugaggacga aaggcgcguu      900

gcacugcccc ucccuuacgg guaugccgau gcuaagcuga ccuacaagca gcuucgggcc      960

gcacugucca aagccggauu gucuaacuuc agcuuugcug guaucgccga cgauaaggag     1020

aaagacacac ucacuaagcu gccagcaugg gagaccuuau ccaaaacacu gaagggcaag     1080

gggcuagaaa ccgaguggga aggaauggcg ggcgcugcca cgggugggga ucccacucuc     1140

cuggacgaga uaucaagagu gcuuaguguc uauaaagaug gcgacgaagu ggauguggag     1200

uugaggaagc ugccucuccc aggaggcgag aaaauggucg aaacacugag cgagaucucu     1260

uucgacaagu uucacgcacu uucccugaaa gccuugcgca agauuguucc gcauauggaa     1320

accgggcucc gauacgauga ggcuugugcc gcagcuggau accaccauuc acagcugcac     1380

gaggccggug cgggcgggca aaaguauuua cccccuuucu acacucggcg ugacaaagac     1440

ggaagaaugg uguucgauga ggagcuggac guaccaagga aucccguggu ccuacgcgcc     1500

cucaaccagg cucggaaggu gcugaaugca cuuguuagag ccuauggcag cccuagugcu     1560

gugcacaucg aaauggccag ggauuugucu aaaccacugg ucggucaucc cacaccgaac     1620

gggggcuaca uugagggacg acgcgacauc gaaaagcucc agaaagagua ucgggagaga     1680

aaugaaaagg auaaggcagc guuugccgcu gagcacggca uagccgcaaa auccagggag     1740

uucgaaaagu uucagcugua ccgugaacaa caggggaaau gcgcuuauag cuuaaagccu     1800

cuggaccuuc aucgcguguu gcacgauauu ggauacgccg agguagacca ugcacucccc     1860

uacucgcggu cuuaugauga cgguaaaaac aauaagguuc uggugcuaac cgaggaaaac     1920

agaaauaaag gcaacaggac ggccuacgag uaucugugug cuuucccagg gggagaaaac     1980

ggcgagcgau ggcggaauuu ugucgcguac guggaaucca acaagucaua ucgccucgcc     2040

aagagaacca ggcugcuucg gaaagauuuc ggugggaagg cagcugagga guuucgagaa     2100

agaaauuuga acgacacacg cuacaucugc aaauucuuua agaauuucgu cgagagguuu     2160

cugcaguuaa aagugggcgc cgauggaacu gccgcuaagc guuguguugu gcucaguggc     2220

caaaccacag cauuccugcg ggccagaugg gggcuucuga agguaagggg agcgagcgac     2280

cgccaccacg cuuuggacgc cgcagucgug gccgcuugcu cccauggcau gguuaaacga     2340

cucgcagauu auucuagaac uaaggaacug gccaaagugc gggacgguuu uccugauccc     2400

gagaccgggg agauugucga cccagcgaug uuccagcagc uacacgcuca uuuccccgau     2460

ccuuggccac acuuuagggg agaacuggag gcccgccucc agcuggacga uagcgcagaa     2520

cuucgugccc aauuggagau guucggcaca uacccggcug aagcccugga gaccuuaaga     2580

ccccucuuug ugucacgggc accucagagg cgcaacggug gcgcugccca caaggagacu     2640

aucuacgcgc agccagaacg acugaaagag caagggggag uaacgcagaa ggugagucuu     2700

gccucccuga agcccacaga caucgauaaa cuaauugacc cucauagaaa ugaaaagcuc     2760

uaugcagcua uacgggccag gcuggaggcu cacggcggga aaagcgauaa ggcauuccca     2820

gccgacaacc ccuugcgcaa accggauaga gacggaaauc cuaccggccc aaucguuagg     2880

gccgucacua uggugauuga caagcugucu gguauccaga uacguggggg aauugcuaag     2940

aacgauacca uguuacgggu ggacgucuuu cgccauaaaa aggauggcaa auuccaccuc     3000

guuccccugu acgugcauca cgcgguggca caggaacuuc cuaaucgagc caucguagcu     3060

uuuaaggacg aggaugagug gacacugauc gacgauaacu uccacuuuuu guucucauuu     3120

caucccaaug acuucauuag aaucacccag aaaaacaagg ccccaauucu cgguuauuac     3180

gcaaguuguc acaggggcac ugggaaucug aaccuauggg cccaugaucg gaaccaaaaa     3240

gucggaaagg acggccagau agaagggauc ggagugaaga cagcuguugc gcuggagaaa     3300

uuucacgugg auguccuugg caauauuuau ccugcacccc cagaacagcg ccauggucuc     3360

gccuga                                                                3366


<210>  2792
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2792
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu ugguaaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggcgcg ugagacugaa uaggcuauuu gaggaaucag gccucauuac ggacuucacc      240

aagaucagua uaaaccugaa uccuuaccag cuucggguca aaggucugac agaugaguug      300

agcaacgaag aacucuuuau cgcccugaag aauaugguga agcaccgcgg gauuuccuau      360

cuggacgaug cgucugacga uggaaacucg ucaguuggcg acuacgcuca gaucgugaaa      420

gagaauagca agcagcucga aacuaaaacc ccaggccaaa uucaguuaga gcgauaucag      480

acauacgggc agcugagagg agauuucacc gucgaaaagg acgguaaaaa gcauaggcuu      540

aucaacgugu uuccgacuag cgccuaucgg uccgaggcau ugcguauacu gcaaacacag      600

caggaguuca auccccagau uaccgaugaa uuuaucaacc gcuaccuaga gauucucacg      660

ggcaagagaa aauauuacca cgggccugga aaugaaaagu cuaggacuga cuacggccgg      720

uaucgaacca gcggugagac acuggauaac aucuucggga uccuuauugg aaaaugcacu      780

uuuuacccag acgaguuccg cgccgcuaag gcauccuaua ccgcccaaga guuuaaccug      840

uugaaugacc ucaacaaucu gacaguaccc accgagacga aaaaguuauc aaaagaacag      900

aagaaccaga uaaucaauua cguuaagaac gagaaagcua ugggcccugc caagcuguuc      960

aaauauauug cgaagcuccu uaguugugau guggcagaca ucaaaggcua cagaauagau     1020

aagagcggga aggccgaaau ucauacuuuu gaggcuuaua ggaaaaugaa gacacuggaa     1080

accuuggaca ucgagcaaau ggaucgggag acucuagaca aacuggccua cguccucaca     1140

cugaauaccg aacgcgaggg aauccaggaa gcacuugagc acgaguucgc ugaugguucu     1200

uucucccaga aacaggugga cgaacuggug caauuuagaa aagccaacuc aagcauuuuc     1260

ggcaaggggu ggcacaauuu uagugucaag uugaugaugg agcucauccc agaacuguac     1320

gagacgucug aggaacagau gaccauuuua acaaggcugg gaaaacagaa gacuaccucc     1380

agcucgaaca aaacaaagua uauagaugag aaacuacuca cugaagagau cuacaauccc     1440

guuguggcga agucuguacg ucaggccauu aagaucguga acgcagcuau caaagaauau     1500

ggcgacuucg auaauauugu cauagagaug gcccgggaaa ccaacgagga cgacgagaag     1560

aaagcuaucc aaaagauuca gaaagccaau aaggaugaaa aagacgcagc caugcugaag     1620

gcugcgaacc aguacaacgg uaaggcagag cuuccucauu ccguguuuca cgggcauaaa     1680

caguuggcca caaagauccg acuguggcac caacagggcg aaagaugccu cuauaccgga     1740

aaaacuauuu caauccacga ucugauaaau aacaguaauc aguucgaggu ugaccauauc     1800

cuuccacuga gcauuacguu ugaugacucc uuggcuaaca aggugcucgu cuacgccaca     1860

gcaaaucagg aaaaaggcca aaggaccccg uaucaggccc uggauucuau ggacgaugcu     1920

uggagcuucc gcgaguuaaa ggccuuugug cgggagucaa agacucugag uaacaaaaag     1980

aaagaauacc uacucaccga ggaagacauc uccaaguucg auguaagaaa gaaguuuauu     2040

gagaggaauc ugguugacac acgcuacgca agccgagugg uccuuaacgc guugcaggaa     2100

cacuuccggg cucauaagau cgauaccaaa gugucugucg ugcgugggca auuuacuuca     2160

cagcugagaa ggcacugggg aauagagaag acacgcgaca cguaucauca ccacgccguu     2220

gacgcacuca uuaucgccgc uaguagccag cugaauuuau ggaaaaagca gaaaaacacc     2280

cugguguccu acucugagga ucaacuuuug gacauugaaa cuggugagcu caucucggau     2340

gacgaauaua aggagagcgu auucaaggcc cccuaccagc auuucgucga uacacugaaa     2400

uccaaggagu uugaagacuc aauacuauuc aguuaucagg uggauucuaa auuuaaucgg     2460

aagaucagcg acgcaaccau uuacgcuaca agacaggcca aaguuggcaa ggauaaagcg     2520

gacgagacuu augugcuggg gaagaucaag gauauuuaca cccaagacgg auacgaugcc     2580

uucaugaaaa ucuauaagaa agacaagucc aaauuucuca uguacaggca cgacccucag     2640

acguucgaaa aggucauaga gccaauucug gaaaacuauc ccaauaagca gaucaacgag     2700

aaaggcaagg aggugccuug uaauccauuu cuuaaauaca aggaggaaca ugguuauauc     2760

cgaaaauacu caaagaaggg gaacggcccc gaaauuaaaa guuugaagua uuacgauucu     2820

aaacugggaa accacaucga cauuaccccg aaggauucca auaacaaagu aguguuacag     2880

aguguuagcc cuuggcgcgc ugacgucuac uucaauaaga caacuggcaa guaugagaua     2940

cucgggcuga aauacgcaga ucuucaauuu gaaaagggaa ccgguacaua uaaaaucucu     3000

caggagaagu acaacgacau uaaaaagaag gagggcgugg auuccgacuc agaguucaaa     3060

uuuacucugu auaagaauga uuugcuccug gugaaagaca ccgaaacgaa ggaacagcaa     3120

cuauuccggu uucugaguag aacaaugccc aaacagaagc acuacgucga gcucaaacca     3180

uaugauaagc agaaguucga agggggagag gcccugauca aaguucuugg caacguggcu     3240

aauagcgguc agugcaagaa aggguugggc aagucgaaca uuuccaucua caaagugagg     3300

accgacguac ugggaaauca acauauaauc aagaacgagg gcgacaagcc uaaauuagau     3360

uucuga                                                                3366


<210>  2793
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2793
augaacggcc uggugcucgg gcuugacauc ggaauuggua gcgucggcgu ugggauacug       60

aauaaggaua ccggagagau cauucacgcc aacuccagaa ucuuccccgc ugcaacagcc      120

gacaauaacg ugguaaggcg gucuucacgc cagggccgac guuugaauca uaggaaaaag      180

cacagaagug ugcgguuaaa agaucuguuu gaauacuaug accuacucac ugauuucagc      240

aaggucucca uuaaccugaa uccuuaccgc cuuagagugg acggucugaa ccaacaguug      300

acgaaugagg aacucuuuau cgcgcugaaa aacauaguua agaggcgggg gaucucuuau      360

cuggaugacg cuucggagga uggaggcgcc gugucaagcg acuacggcaa ggcagucgaa      420

gagaaucgca aacucuuagc cgagcagacc ccagggcaga uucaacugga acgauucgag      480

aaguauggac aggugagagg ugauuuuaca guaguugaaa acggcgagaa aaggcggcuu      540

aucaaugugu ucaguacuuc cgcuuacaag aaagaggcag aacguauuuu gcgcaagcag      600

caggaguaua acaaucaaau cauggacgaa uuuauagccg auuaccugac cauucuaaca      660

gggaagagaa aauacuauca uggaccgggc aacgagaagu cuaggaccga cuacggucgg      720

uuccgaacug acgggacaac ccucgauaac aucuuuggca uucugaucgg aaaaugcacg      780

uucuaucccg aagaguaccg cgcuagcaag gccuccuaua cugcgcagga guuuaaucuu      840

cugaacgacu ugaauaaccu caccgucccu acagaaacua aaaagcuguc agaggaacag      900

aaaaagacca ucguggagua cgcaaagagu gccaaaacau uaggcgcuag cacccugcuc      960

aaguauauug ccaaaaugau agaugcaucu guggaccaga ucagagggua cagggucgau     1020

guuaauaaca agccagaaau gcacacguuc gagguguacc ggaaaaugca acuucuggag     1080

acuauucccg uaggagaauu uucccgcgag guguuggacg aacuagcuca uauccugaca     1140

cucaauaccg agagagaagg uauagaggag gccauuaaca cuaagcugau ggaugucuuc     1200

ucacaggacc agauccuuga acuggugcaa uucaggaaga auaacagcag uuuguuuucu     1260

aaaggcuggc acaauuucuc ccucaagcug augaucgagu uaauuccuga acuguaugag     1320

acaagcgagg aacagauggc gauccuaacc cgucucggga aacagaaguu uaaagagacg     1380

ucgaagcgga ccaaguacau ugaugaacag gagguuacag aagagauaua uaacccagug     1440

gucgccaaau cugugcgaca agcaaucaag auuaucaaug aggcuacuaa aaaguacgga     1500

aucuucgaca acauuguaau agaaauggcc agagagaaua acgaagagga agcuaaaaag     1560

gauuauauca agaggcagaa agccaaccac gacgagaaga augcagccau ggagaaagcu     1620

gcguuucagu acaacggcga aaaggagcug cccgacucca uuuuccaugg ucacaaagaa     1680

cuugcaacca agguucgcuu guggcaucag caaggggaga aaugucugua uacaggcaag     1740

aauaucccua uuucagaucu cauccacaac caguacaagu acgaaauaga ccacauccug     1800

ccacuuaguc ugagcuuuga ugacuccuug ucuaauaaag ugcucguccu ggccacugcu     1860

aaccaggaga agggacggag aaccccguuc caggccuuag auagcaugga cgaugcaugg     1920

ucauauaggg aguuuaaaag uuacgugaag gacuccaaaa gccugggcaa uaagaagaaa     1980

gauuaucuac ucacagaaga ggacauuucu aagaucgaag ucaaacaaaa guucauugag     2040

cgcaaccugg uggauacccg auacucaagu cggguugugc uuaauacuuu gcaggacuuu     2100

uauaaagccc auaaguucga uacgacaauc agcguagucc gugggcaguu uaccucccag     2160

cugagaagga aguggggacu cgagaaaucu cgcgaaacuu accaccauca cgcuguggac     2220

gcccugauaa uugcagcguc gagccaauua cggcugugga agaaacagaa caauccccuu     2280

aucuccuaua aggaggguca guucguugac ucagaaaccg gcgagauuau caguuugaca     2340

gaugacgaau acaaagagcu cguguucaag gcuccuuacg aucauuuugu cgacacccug     2400

agaucuaaga aauucgagga uagcauacua uuuuccuauc aaguggacuc aaaguucaac     2460

aggaaaauca gcgaugccac uauuuacgca acacgaaagg ccaaacugga caaggauaaa     2520

ucugaagaga cguauguacu cgggaagauc aaggacauuu acucccagag uggauaugau     2580

gcuuuuauca aaauauacaa uaaggacaaa agcaaguucc ugauguauca caaagaucca     2640

cagaccuuug aaaaggugau ugaggaaauc cuucgcacuu accccucuaa ggaguucaac     2700

gagaaaaaua aggaaguuuc cugcaacccu uuugagaaau acaagcagga aaacggccca     2760

auccggaaau auucaaagaa ggguaauggg cccgagauua aaaguuugaa guacuaugac     2820

aaaaacaucc cggagagcuu cauuaauaua acaccuucga acuccaagaa uaaagucauc     2880

cugagacaau uaaaccccug gaggaccgac guguacuuua aucaccagac agagaaguau     2940

gagcuccugg gccuuaagua cgccgaucug caguucgaga aaaagacugg aaccuauucu     3000

auuacgaucg acaaauacaa cgaaauuaag gaaaaagagg gcgugagcga gcauucagaa     3060

uuuaaguuca ccuuguacaa gaaugaucuc cugcuaauca aagacacaga gauaaaggaa     3120

gaacagaucu uccguuuucu gagucgcacu augccaaacc aaaaacacua ugucgaacuc     3180

aagccuuacu auaaagggaa uuucgaggga aaggagcgga uuaaaguuuu uaacaccaca     3240

uccaauggua gaugucugaa gggccuuagg aagaaagggu ugucuaucua caagauuaaa     3300

acugaugugc ugggaaacaa gcauuucauc aaaaaggaag gcgagaagcc caaauuaaag     3360

uuuuga                                                                3366


<210>  2794
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2794
augaacggca agauccuggg gcucgacauu ggaguggcca gcgucggugu uggcauacuu       60

gauaaaaaga ccggggaaau cauucacgcu uccucuagaa ucuuccccgc agccacagcg      120

gacucaaaug uggaaaggcg gggauuucgc cagggccgac gucuggguag aaggaaaaag      180

caucggaaag uacgcuuggc ugauuuauuc agugacacug ggcugauuac ggauuuuagc      240

aaggugucca ucaaccuaaa uccuuacgag cucagaauaa agggacugaa cgaaaaacuu      300

accaaugagg aacuguucau cgccuugaag aacauuguca aaaggcgggg caucucuuau      360

cucgacgaug caaaugagga cggcgagucg ucaagcagug aauacgggaa ggccguggag      420

gaaaaccgca aacugcuggc ugauaagaca ccaggacaaa uucagcucga gcgauuugag      480

aaguaugguc agguuagagg cgacuucacu aucgaagaga auggggaaaa acacagguua      540

cugaacgugu uuuccaccuc ugcauacaag aaagaggccg aacggauacu uacaaagcag      600

caagauuaua aucaggacau uaccgaugag uucauccagg cuuacuugac uauucugaca      660

ggaaaacgua aguauuacca uggcccgggu aacgagaaaa gccgcaccga cuacgggaga      720

uuuaggacgg acggaacuac ccuagauaau aucuucggca uccucauugg caagugcaca      780

uuuuaucccg aagaguaccg ggccgcgaag gcauccuaua cugcccagga guucaaccug      840

cuuaaugacc ugaacaacuu gaccgucccu acagagacca aaaagcucuc agaagaacaa      900

aaacgacaga uaaucgagua cgcuaagggg gccaaaacgc ugggagcagc uacuuuacug      960

aaguauauug ccaagcucgu ggaugguagu guagaagaca ucaaaggcua ccgcauagau     1020

aagagcgaga aaccagaaau gcacacauuu gacauuuaua gaaagaugca gacccuugag     1080

acuguugaug uggaaaaacu gucuagggag gucuuggacg agcuagcgca uauccugaca     1140

cucaauaccg aacgggaggg gaucgaagag gccauuaagg uguccuucau caagcgcgag     1200

uucgaacagg aucaaauugc agagcuggug ucauuuagaa aaagcaacag uucucuuuuc     1260

ggaaagggcu ggcacaauuu uuccauaaaa cugaugacgg aauugauccc cgagcucuac     1320

gaaaccagcg aggagcagau gacaauucug acuagguuag guaagcagaa aaccaaggcu     1380

cguucgaagc ggacaaaaua caucgacgaa aaggagcuga cugaugaaau cuauaacccu     1440

gucguugcca aaucugugcg acaagcuauu aagauaauca aucuagccac caaaaaguac     1500

gggguauucg acaacauugu gaucgagaug gcaagagaaa auaacgagga ggacgccaaa     1560

aaggauuaug ucaagaggca gaaagcuaau gaagacgaga agaacgcggc aauggaaaaa     1620

gccgcucacc aguacaaugg caagaaagag cucccagaua acguguuuca uggacacaag     1680

gaacuggcca caaagauucg ccuuuggcau cagcaaggcg agaaauguuu guauaccggg     1740

aagaauaucc ccauauccga ccugauccac aaccaguaca aauaugagau ugaucacauc     1800

cucccucugu cacuuaguuu cgacgauagc cuggcaaaua agguuuuggu gcucgccacu     1860

gcuaaccagg aaaaaggaca gcggacgcca uuucaagccc uggacuccau ggaugacgca     1920

uggucuuaca gagaguucaa ggcguacguc aggggugcuc gcgccuuaag caacaagaaa     1980

aaggauuauc ugcuaaauga agaggacauu aacaaaaucg aggugaagca gaaauuuaua     2040

gaacgaaauc ucguagauac acgguacuca agucguguug ugcugaacgc acuucaggac     2100

uucuauaagu ugaaugacuu ugauaccaag auuuccgucg ugagaggcca guucacuagc     2160

caacugaggc gcaaauggcg gaucgacaag ucuagagaga ccuaccauca ccaugccguc     2220

gaugcucuca uuaucgccgc aucaagucag cugagguuau ggaaaaagca ggggaacccg     2280

cugauaagcu auaaagaaaa ucaguuugug gacuccgaga caggagaaau cauuucucuu     2340

accgaugacg aguacaagga guugguuuuc cgagcucccu augaucacuu cguggacacu     2400

guaucgagca aaaaguuuga agaucgcauc cucuucuccu accaagucga cucaaaguac     2460

aaccggaaaa uuagugaugc cacaaucuau ucuacgagaa aggcgaaacu gggcaaggac     2520

aaaagcgagg aaaccuacgu gcuagguaag auaaaggaua uuuauacuca gacaggguac     2580

gacgccuuua ucaaacugua uaagaaagac aaguccaaau uccucaugua ccauaaggau     2640

ccuaucaccu uugagaaggu uauugaagag auccugaaaa cauauccaga caaggagauu     2700

aaugaaaaag gcaaggaggu ggcuugcaac cccuucgaaa aauacaggca ggagaaugga     2760

ccucuucgua aguacucaaa gaaaggcaag gggccagaaa uaaagagcuu gaaguauuac     2820

gauaacaaac ugggaaauca caucgacauu acucccgaua acucugagaa ucaagucauc     2880

uuacaguccc ucaagccgug gcgcaccgac guguauuuua accacaagac gaaaauuuac     2940

gagcugaugg gucuuaagua uagugaucug agcuucgaaa aaggcucugg gaaguaccgg     3000

aucuccuugg acaaauauaa cguaauaaag aaaaaggagg gagugcauaa ggaaucagag     3060

uuuaaauuca cccucuacaa gaaugaucug auccuaauua aagaccugga gaagagugaa     3120

cagcagcucu uuagauacaa cagcaggaau gauacaucga aacacuaugu ugagcugaag     3180

ccuuacgaca aggcaaaauu cgaaggcaac caaccccuua uggccuuguu ugguaauguc     3240

gcuaaagggg gccagugucu gaaaggauua aacaaggcca auaucuccau uuauaaagug     3300

cagacugacg ugcucggcaa caagcgauuc aucaagaaag agggggaugc accaaagcug     3360

gaguucuga                                                             3369


<210>  2795
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2795
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu ugguaaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggauug ugcgccugaa uagacuauuu gaggaaucag gccucaucac ggacuucacc      240

aagauaagua ucaaccugaa uccuuaccag cuuaggguca aaggucugac agaugaguug      300

agcaacgaag aacucuuuau ugcccugaag aauaugguga agcaccgggg gaucuccuau      360

cuggacgaug cgucugacga uggaaacucg ucaguuggcg acuacgcuca gauugugaaa      420

gagaauagca agcagcucga aacuaaaacc ccaggccaaa uccaguuaga gcgcuaucag      480

acauacgggc agcugcgagg agauuucacc gucgaaaagg acgguaaaaa gcauagacuu      540

auaaacgugu uuccgacaag ugccuauagg uccgaggcau ugcggauucu gcaaacucag      600

caggaguuca auucucagau caccgaugaa uuuauuaacc guuaccuaga gauccucacg      660

ggcaagcgca aauauuacca cgggcccgga aaugaaaaga gcagaacuga cuacggcagg      720

uaucggacca acggugagac acuggauaac aucuucggga uucuuauagg aaaaugcacu      780

uuuuacccug acgaguuccg agccgcuaag gcauccuaua ccgcccaaga guuuaaucug      840

uugaacgacc ucaauaaccu gacaguacca accgagacga aaaaguuauc aaaagaacag      900

aagaaucaga ucauuaacua cguuaagaau gagaaaguga ugggccccgc uaagcuguuc      960

aaauauaucg ccaagcuccu uaguugugau gucgcggaca uaaaaggcca ucgcauugau     1020

aagagcggga aggcagaaau ccacacuuuu gaggccuaca gaaaaaugaa gacacuggaa     1080

accuuggaca ucgagcaaau ggauagggag acucuagaca aacuggcuua ugugcucaca     1140

cugaacaccg aacgggaggg uauucaggaa gcccuugagc acgaguucgc agauggaucu     1200

uucucccaga aacaggugga cgaacugguc caauuucgca aggcuaauuc aagcaucuuc     1260

ggcaaggggu ggcauaacuu uaguguuaag uugaugaugg agcucauucc ugaacuguac     1320

gagacgucug aggaacagau gaccauauua acaagacugg gaaaacagaa gacuaccucc     1380

agcucgaaua aaacaaagua caucgaugag aaacuacuca cugaagagau uuauaaccca     1440

gugguagcca agucugugag gcaggcgauc aagaucguca augccgcaau uaaagaauac     1500

ggcgacuucg auaacauagu gaucgagaug gcucgugaaa ccaaugagga cgacgagaag     1560

aaagccauuc aaaagaucca gaaagcuaac aaggaugaaa aagacgccgc aaugcugaag     1620

gccgcuaacc aguauaaugg uaaggcggag cuuccccacu ccguuuuuca ugggcacaaa     1680

caguuggcaa caaagauucg gcuguggcac caacagggcg aacgaugccu cuacaccgga     1740

aaaacuaucu caauacauga ucugaucaac aauccuaacc aguucgaggu ggaccacauu     1800

cuuccacuga guaucacguu ugaugacagc uuggccaaua agguccucgu guaugcuaca     1860

gccaaccagg aaaaaggcca aagaaccccg uaccaggcac uggauuccau ggacgaugcc     1920

uggucuuuca gggaguuaaa ggcuuuugua cgcgagagca agacucuguc aaauaaaaag     1980

aaagaauauc uacucaccga ggaagacauu aguaaguucg auguucggaa aaaguuuauc     2040

gagagaaacc ugguggacac aagguacgcc ucccgcgucg ugcuuaaugc auugcaggaa     2100

cauuuccgag cgcacaagau agauaccaaa gucagcgugg uucgggggca auuuacuucu     2160

cagcugcgua gacauugggg aauugagaag acaagggaca cguaccacca ccaugcugug     2220

gacgcccuca ucauugcagc cucaagucag cugaacuuau ggaaaaagca gaaaaauacc     2280

cugguaagcu auuccgagga acaacuuuug gauaucgaga cuggugaacu cauaucugac     2340

gaugaguaca aggagucggu cuucaaggcu cccuaucagc acuucgugga cacacugaaa     2400

agcaaggaau uugaggauuc cauccuauuc ucauaccagg uugacaguaa auuuaaccgc     2460

aagauuucug augccaccau cuaugcaaca cggcaggcua aagugggcaa ggacaaaaag     2520

gaugaaacuu acguccuggg gaagauuaaa gacaucuaua cccaagaugg auacgacgcc     2580

uucaugaaga uauacaaaaa ggauaaaagc aaguuucuca uguauagaca ugacccucag     2640

acguucgaga aggugauuga accaauccug gagaauuacc ccaacaaaga gaugaaugaa     2700

aagggcaaag agguaccuug uaacccauuu cuuaaguaua aagaggaaca cgguuacauc     2760

aggaaguauu ccaagaaagg gaacggcccc gaaauuaagu cauugaaaua cuaugacagc     2820

aagcuguuag gaaauccgau cgauauuacc ccugagaacu cuaaaaauaa ggugguucuc     2880

cagucccuga agcccuggcg aacagacguc uacuucaaca aaaauacugg caaguacgag     2940

auacuugggc ugaaauaugc ggauuugcag uuugaaaaga aaaccggaac auacaagauc     3000

agucaagaga aguauaacgg uauuaugaaa gaagagggcg uggacagcga uucugaguuc     3060

aaguuuacuc ucuacaaaaa ugaccugcua cuggugaagg auaccgaaac gaaagagcag     3120

caacucuucc gcuuucuguc ccggacaaug ccaaacguca aguauuacgu ugaacuuaaa     3180

ccuuauucaa aggacaaguu cgagaaaaau gaaaguuuga ucgagauucu ggggagcgcc     3240

gauaagucgg gaagaugcau caaaggcuua gguaagucca acauaucuau cuacaaagug     3300

aggaccgacg ugcucgggaa ucagcacauu aucaagaacg agggcgauaa gcccaaacug     3360

gacuucuga                                                             3369


<210>  2796
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2796
augagcaacg gcaagauccu ggggcucgac auuggaauag ccuccguggg ugucggcguu       60

aucgaugcuc agaccgggga gauuauccac gcaucuucaa gaauuuuccc cagugccaau      120

gcggcuaaca augccgaaag gcggacauuu cgcggaagcc gacgucuuau cagaaggaaa      180

aagcaucgga uaaaacgccu ggacgauuug uucaacgacu uucacaucaa uuuagauggc      240

gagaugucca cugacaaccc uuacgugcug agaguaaagg gucuaucuca aaaacucacg      300

guggaagaac uguauauuuc gaucaagaau auuaugaaga ggcgggggau cucauaccuu      360

gaugacgcag aaagcgauaa cgaggccgga cgcagugacu augcuaaagc aauagagcga      420

aauagacagc uguugaccuc caagacacca ggcgaaauuc agcucgagag gcuggaaaaa      480

uacggccagc ugcgggggaa cuucacuauc auugaugagg agggacaauc ucagcagauc      540

aucaaugucu uuagcaccuc cgacuaugug aaggaaguug agaaaauucu cgauugccag      600

aagauguacc auaaguucau aucagacgaa uuuugugaca aauuaaucga gcugcuucgu      660

gaaaagcgca aauauuacgu ggguccgggc aacgagaaga guagaacaga uuacgggauu      720

uauaggaccg acggaacuac auuggagaau cuguucggca uccuaauagg uaaaugcacc      780

uuuuaccccg aucaauaucg gagcucucga gccuccuaca cggcucagga guucaacuuu      840

cucaaugacc ugaacaaccu uacugucccu accgagacaa agaaacuguc acaggaacag      900

aaggaguucu uggugaauua ugccaaggaa acuagcguac ucggggcggg aaaaauucug      960

caacagaucg caaaguuagc cgauuguaaa guugaggaca uccgcggcua cagacuggau     1020

aacaaggaca aaccagagcu ccacauuuuu gaaaccuaua gggcuaugaa gggccuugug     1080

ccccuggucg auaucggggu guugagucgg gagcagcuag acauucuggc cgauauacuc     1140

acacugaaua ccgacuucga aggaauccgc gaggcacuua agaaacaacu gccuaacgug     1200

uucgacgaaa agcaggucaa agguuuggcu ucuuuuagaa aguccaaaag ccaacucuuc     1260

gccaagggcu ggcauaaucu gucgcagaag auuauguuag agguuauccc agagcuguac     1320

gcgacgucug augaacagau gacuauccua acaaggcucg ggaaauuuga gaaguccuca     1380

guggccgaau accccaguag cauuaacgua gacgagauaa ccgaugagau cuauaauccu     1440

guggucgcaa aauccauucg ucagacuauc aagauuauca acgcuucuau caaaaagugg     1500

ggagaguucg accagauagu gauugagaug ccacgggauc gaaaugaaga cgaggaaaag     1560

aaaagaaucg ccgauggcca gaaggcuaac gccaaagaga aggcagacag cauucugagg     1620

gccgcugagc uuuacugcgc ggguaaaguu uugccggauu auguguacaa ugggcacaac     1680

caacuggcaa caaagauccg ccucugguau cagcagggcg aacgguguau auacaccgga     1740

cagcccauuu caauccacga ccugauucau aaucaaaacc aguaugagau cgaucacaua     1800

cuuccucuga guuugacguu ugacgauucc cucagcaaua aaguccuggu guuagccacc     1860

gcuaaccagg aaaaggccca gagaacacca uacaacuacc ugaagucugc aacuucagcc     1920

uggaguuaua gggaguucaa agacuacgua accaagcgca aaggcaucgg gaagaaaaag     1980

ugcgaauauc uaacauuuga ggaggacauu aauggauucg aaguucgaag caaguuuauc     2040

caacggaacc ucguggauac ucguuacgcu uccaaaguca uucugaaugc ccuucaggac     2100

uauuucaaga ucucugguau acagaccaaa gugucggucg ugagaggcca auuuacaagc     2160

caguugaggc auaagugggg gauugagaaa acccgcgaaa cuuaccacca ccaugcaguu     2220

gaugcgcuga ucaucgcugc cuccucacag cuccggcugu ggaagaagca ggagaguccc     2280

uuagugguag acuaucaaga aggaagacag gucgaucugg agacgggcga gauucuugaa     2340

uugacagacg agcaguacaa agaacucgug uaccagccuc cauaucaagg uuucguuaac     2400

accaucucua gcuccgcauu ugauaaugag auucuguucu cauaccaggu ggacagcaag     2460

gucaacagga aaauaucuga ugccacuauc uaugcuaccc gaaaugccca gcuagggaag     2520

gacaaaacag agggcauuua cgugcuggga aagaucaagg auauuuauac ccaggcaggc     2580

uacgaggcuu uccucaaacg cuauacuaag gacaaaacau ccuuucugau guaccacaag     2640

gaucuugaca cgugggaaaa aguaaucgaa auaaucuugc gggacuacag agaguaugau     2700

gaaaagggga aagagauugg aaaccccuuc gagagguacc gucgcgaaaa ugguuaugug     2760

aagaaguaca gucggaaagg caacgggacc gccaucaaga gccugaaaua uuacgacaau     2820

aaguuaggaa accauauuga uaucacuccg gaaaauucua gaaacgcggu uguccuccaa     2880

ucccugaaac cuuggaggac agacguguau uuuaauaagg aaaccggcaa guacgaguuc     2940

cuugguauaa aauacucaga ucugaguuuu gaaaagggga caggcgagua uggaauuagc     3000

caggagaaau acgacucgau caagaucgcc gaaggcgugg cuaaaaaguc cauuuucaag     3060

uuuacuuugu auaaacagga ucuccuguuc auaaaggaca ucgagaacaa cuuugggaaa     3120

cuacugcgau ucaccucuaa gaaugauacg agcaaacacu acgucgaacu caagcccuau     3180

gacaagaaca aauuuggaac cgaggaacca cugcuuccug uuuugggcaa uguggcaaag     3240

ucaggucaau guauuaaagg gcugaacaag aguaauaucu ccauuuacaa agugcggaca     3300

gauaucuuag gauaucgcca uuucauaaag caggagggcg agcaccccca gcucaaguuu     3360

aaaaaguga                                                             3369


<210>  2797
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2797
augagcaacg gcaagauccu ggggcucgac auuggaauag ccuccguggg ugucggcguu       60

aucgaugcuc agaccgggga gauuauccac gcaucuucaa gaauuuuccc cagugccaau      120

gcggcuaaca augccgaaag gcggacauuu cgcggaagcc gacgucuuau cagaaggaaa      180

aagcaucgga uaaaacgccu ggacgauuug uucaacgacu uucacaucaa uuuagauggc      240

gagaugucca cugacaaccc uuacgugcug agaguaaagg gucuaucuca aaaacucacg      300

guggaagaac uguauauuuc gaucaagaau auuaugaaga ggcgggggau cucauaccuu      360

gaugacgcag aaagcgauaa cgaggccgga cgcagugacu augcuaaagc aauagagcga      420

aauagacagc uguugaccuc caagacacca ggcgaaauuc agcucgagag gcuggaaaaa      480

uacggccagc ugcgggggaa cuucacuauc auugaugagg agggacaauc ucagcagauc      540

aucaaugucu uuagcaccuc cgacuaugug aaggaaguug agaaaauucu cgauugccag      600

aagauguacc auaaguucau aucagacgaa uuuugugaca aauuaaucga gcugcuucgu      660

gaaaagcgca aauauuacgu ggguccgggc aacgagaaga guagaacaga uuacgggauu      720

uauaggaccg acggaacuac auuggagaau cuguucggca uccuaauagg uaaaugcacc      780

uuuuaccccg aucaauaucg gagcucucga gccuccuaca cggcucagga guucaacuuu      840

cucaaugacc ugaacaaccu uacugucccu accgagacaa agaaacuguc acaggaacag      900

aaggaguucu uggugaauua ugccaaggaa acuagcguac ucggggcggg aaaaauucug      960

caacagaucg caaaguuagc cgauuguaaa guugaggaca uccgcggcua cagacuggau     1020

aacaaggaca aaccagagcu ccacaccuuu gaaacauaua gggcuaugaa gggccuugug     1080

ccccuggucg auauuggggu guugagucgg gagcagcuag acauccuggc cgauauucuc     1140

acccugaaua cggacuucga aggaauacgc gaggcacuua agaaacaacu gccuaacgug     1200

uucgacgaaa agcaggucaa agguuuggcu ucuuuuagaa aguccaaaag ccaacucuuc     1260

gccaagggcu ggcauaaucu gucgcagaag aucauguuag agguuauucc agagcuguac     1320

gcgacuucug augaacagau gacaauccua accaggcucg ggaaauuuga gaaguccuca     1380

guggccgaau accccaguag caucaacgua gacgagauua cugaugagau auauaauccu     1440

guggucgcaa aauccauccg ucagacaauu aagaucauua acgcuucuau caaaaagugg     1500

ggagaguucg accagauagu gaucgagaug ccacgggauc gaaaugaaga cgaggaaaag     1560

aaaagaauug ccgauggcca gaaggcuaac gccaaagaga aggcagacag cauccugagg     1620

gccgcugagc uuuacugcgc ggguaaaguu uugccggauu auguguacaa ugggcacaac     1680

caacuggcaa ccaagauucg ccucugguau cagcagggcg aacgguguau cuacacggga     1740

cagcccauau caauucacga ccugauccau aaucaaaacc aguaugagau ugaucacauc     1800

cuuccucuga guuugaccuu ugacgauucc cucagcaaua aaguccuggu guuagccaca     1860

gcuaaccagg aaaaggccca gagaacucca uacaacuacc ugaagucugc aaccucagcc     1920

uggaguuaua gggaguucaa agacuacgua acaaagcgca aaggcauagg gaagaaaaag     1980

ugcgaauauc uaacuuuuga ggaggacauc aauggauucg aaguucgaag caaguuuauu     2040

caacggaacc ucguggauac ccguuacgcu uccaaaguca uccugaaugc ccuucaggac     2100

uauuucaaga uuucugguau ccagacaaaa gugucggucg ugagaggcca auuuaccagc     2160

caguugaggc auaagugggg gauagagaaa acucgcgaaa cguaccacca ccaugcaguu     2220

gaugcgcuga uuaucgcugc cuccucacag cuccggcugu ggaagaagca ggagaguccc     2280

uuagugguag acuaucaaga aggaagacag gucgaucugg agacaggcga gauccuugaa     2340

uugaccgacg agcaguacaa agaacucgug uaccagccuc cauaucaagg uuucguuaac     2400

acuauuucua gcuccgcauu ugauaaugag auccuguucu cauaccaggu ggacagcaag     2460

gucaacagga aaauuucuga ugccaccaua uaugcuacac gaaaugccca gcuagggaag     2520

gacaaaaccg agggcaucua cgugcuggga aagauuaagg auaucuauac ucaggcaggc     2580

uacgaggcuu uccucaaacg cuauacaaag gacaaaacgu ccuuucugau guaccacaag     2640

gaucuugaca ccugggaaaa aguaauugaa aucauauugc gggacuacag agaguaugau     2700

gaaaagggga aagagaucgg aaaccccuuc gagagguacc gucgcgaaaa ugguuaugug     2760

aagaaguaca gucggaaagg caacgggacu gccauuaaga gccugaaaua uuacgacaau     2820

aaguuaggaa accauaucga uauuacaccg gaaaauucua gaaacgcggu uguccuccaa     2880

ucccugaaac cuuggaggac cgacguguau uuuaauaagg aaacaggcaa guacgaguuc     2940

cuugguauca aauacucaga ucugaguuuu gaaaagggga cuggcgagua uggaauaagc     3000

caggagaaau acgacucgau uaagaucgcc gaaggcgugg cuaaaaaguc caucuucaag     3060

uuuaccuugu auaaacagga ucuccuguuc auuaaggaca uagagaacaa cuuugggaaa     3120

cuacugcgau ucacgucuaa gaaugauacc agcaaacacu acgucgaacu caagcccuau     3180

gacaagaaca aauuuggaac agaggaacca cugcuuccug uuuugggcaa uguggcaaag     3240

ucaggucaau guaucaaagg gcugaacaag aguaauauuu ccaucuacaa agugcggacu     3300

gauauuuuag gauaucgcca uuucaucaag caggagggcg agcaccccca gcucaaguuu     3360

aaaaaguga                                                             3369


<210>  2798
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2798
auggccaaga uccugggccu cgaccuuggg accaacagca uuggaugggc ucugauagau       60

gacgugcaga auaaaaucuu gggugucggc uccagaauuu uccccauggg gguugagaac      120

uuaggagaug gcgacgguga aaucucuaag aaugcaucaa ggacagggac ucggggagug      180

cgccgacaau uuuuccguag aaggcugcgg aaaaagguac uacucaaagc ccugagugag      240

aacaauaugu gcccuaugau uacgagcgau uuugacaacu ggaagaagac caaacaguuc      300

ccauccgaaa aacuugcguc uugguuugcu cugaauccgu acgaguugcg caacaaagcc      360

cucucggaaa agcugacacu ggaggagauc ggcagaauau ucuaucaccu cauccagagg      420

cggggcuuuu uaucaaauag ccgcaaaggg ggaacugaug acggugcaau uuucaagggc      480

aaccccaagg aagggaaaau cggaauuacc gauacacagg agaguaucca agacaagacc      540

cugggcuccu accuuuuuga aauauauccu aaagagaauc agccauucca gaacgguuug      600

gagcgaauua gaaauaggua cacugugcgg aagauguaug ucgaugaauu ugagaugauc      660

ugggaaaaac aggcccaaua cuauucuaac cugaaugagg accuaaagac acucuucggg      720

ggacguaaac uggauggcua caaggaagac ggcauucuuu uucaucagcg cccccugaga      780

agccagaagc acuugguggg gaacuguguu uucgagccua acaaaaccaa gugcccaauc      840

uccgcuaucc ccuucgagca guuuagggug uggcaauggg ucaauacggu ggaauacaac      900

ggaaagaaga uuucacaggu agagaaagaa aagauaguug aguuccucua ugcaaaugac      960

aagacugauu uuaaaaagau ccggaaagcc auugguaagg aaaguaccga guucaaauuu     1020

aacuacaagg acgaugacaa gaucgugggc acauauacug ucagccaucu gucuaauaaa     1080

aaguacuucg ggaaaaagug guuugaguuc uccgaaaaag agcaggaaga uauauggcac     1140

auuuuauacu uuuucgacuc aaagagcaac cugaaggagu augcuaucaa aaauuggaac     1200

uuuaccgagg aacaagccga gaguaucgcg aaguucaaug ugaaagaugg auauucuucc     1260

cucagccgaa aggcaauuuc gaacauccuu ccuuuucuga aauugggcuu cacauacgac     1320

guggccgucg uucuaggugg gauuaagaau guguucggcc augaauggga gaaacugucu     1380

gaugaaaagc gcaacuuucu cauagacaau guagagggaa ucgugagauc caagaccaaa     1440

ggcggguuca uugaugucau caaggacauc cugaggaacg acuacaauau uucagauaac     1500

cagcuucgga aacuguauca ccacagugcu acgauauacg uggccgaguu gcucgaaaag     1560

cugccaguug gaaaagaggc agacaaggaa auccagagca uucgcaaccc gaucgugauu     1620

acugcuuuau uugagcugag aaagcuaguc aaugaacuca ucgaugacuu cgguuccaua     1680

gaugagauca aaguggagau ggccagggac cugaagauuu cuaaaagcca gcguaacaag     1740

auccggcgag aacaaaagag acuugagagg gaaaaugauc gcguaaaggc gcgguugcug     1800

gaggaaggcc agagaauuac acaugacaac auccuccugu auaaacuuug ggaggagugu     1860

aaaaagaccu gccccuacac ugggaauccu auaccacugu auaaauuguu uacaggagaa     1920

auucagaucg agcacauuca ucccugguca aggagucuca acgauuccuu cgccaauaag     1980

acccuguguu acgcagacga aaaccgcaaa aagggcgaua agacgccuua ugaguuuuac     2040

gguaaugacg aaagcaacug gucugcuauc aaagagcgag ccuuaaagcu guucucagau     2100

accaaagagu acccaaaugc uuaucagaag uuuaaacggu ucguucaaca caaguuugac     2160

gacgauuuca guagccguca gcuaaacgac acaagauaca uauccaaaga agccaagaau     2220

uaucucucua agaucugcaa aaagguggca gucucgcccg ggcaggccac uagcaaccug     2280

aggcagaaau ggggcaugaa uaacauucuu aacgaugaga augaaaagac ccgcgaggac     2340

caccggcauc acgcuaucga ugcguuggug auggcaugua caaaaauuuc cuacguccaa     2400

gagcugucaa aguggaacag auauaauagg aacgccgaac ucaagaaauu uccgcugccu     2460

ugggagacuu ucuggaguga cgcuaaucga gccguggaua agaucuuaau aucucauaaa     2520

aagguuagca acgacauuac cggacgcaca cacaucuccg agaaaaaugg caagaaguac     2580

aaaaacgugg ggguagcagc ccggggacag cugcauaagg aaaccgucua ugguaaaaga     2640

acuuucaaug gcgaagaggc uuuucacgug aggaagacga ucgauucacu uacaaccgag     2700

aaacaguugg aaaagguugu ggacgagagc auuaagcucc ugaucaaaaa gcguguccag     2760

gaacuagggg gauucgugaa aggcgccauu cccgcaaaca ccuuuuucgu aguggaugag     2820

aaugguauaa agcaaccaca gaucuuucug ccuaacaaaa auggggcgcc cguuccaguc     2880

cucaaggugc gcaugaagga aaacauuggc ggagcugaga aacugaagga gaaugugaac     2940

caguggguca acccccggaa uaaccaccau guucuuaucu acaaagacga aaagggcaau     3000

uugaaagagg aaguggugac uuucuggaca guagucgaga gaaagaggac cgggcaaucu     3060

gccuaccagc ugccuuuuga uggaaaagaa auugugacua cauuacacau caacgacaug     3120

uuccuccugg gccuuaauga ggaggaaaua aacugggaga auccagacua ugaaguucug     3180

aaggagcauu uguaccgagu gcagaaguuu acguccggug auuauuacuu ccggaucgca     3240

aaaaccagua gcauucagaa cgccaucgag aagcaacaga uuaauucuuu uggguucggc     3300

aaaaacggau gguccacuca caauccgauc aaggucaaaa uaucagugag ugguaagauu     3360

agcaagguuu ga                                                         3372


<210>  2799
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2799
augaacggcc uggugcucgg gcuugacauc ggaauuggua gcgucggcgu ugggauacug       60

aauaaggaua ccggagagau cauucacaca aacuccagaa ucuuccccgc cgcuacugca      120

gacaauaacg uggaaaggcg gaauagucgc caggcccgac guuugaacag aaggaaaaag      180

caucggggcg cgcgcuuaca cgaucuguuu gaggacuacg aucuacucau ugacuucuca      240

aaaguaagua ucaaucugaa cccuuauaga cuuagggugc aaggucugga uaauccauug      300

acgaccgaag aacucuuuau agcucugaag aacaucguca aacggcgcgg gauuagcuac      360

cuggacgaug ccuccgaaaa uggaggcgca gugucuucgg acuauggcaa ggccguugag      420

gagaaccgaa agcucuuagc ugaacagaca ccggggcaga uccagcugga gagauucgaa      480

aaauacggac aagugagggg ugauuuuacu gucgaggaga agggcgacaa acaucggcuu      540

auuaaugugu ucucaaccag cgcauaucgu aaggaagccg agcgcaucuu gagaaaacag      600

caggaauuua acaaucagau aacagauaag uucauugagg acuaccugaa gauccuaacc      660

gagaaaagga aguauuacca cgggcccgga aacgagaaaa gucggacuga cuacggccga      720

uuucgcacag augguaccac gcucgacaac auuuucggga uccugaucgg aaagugcacu      780

uuuuauccua augaguacag agcuuccaaa gccucuuaua ccgcgcaaga guucaaccuu      840

cugaaugauu ugaacaaucu cacaguacca acugagacca agaaacugag cgaggaacag      900

aagaagacaa uuguugaaua cgcaaaaucc gccaagaccu uaggcgcuuc aacgcugcuc      960

aaauauauag ccaagaugau cgacgcaagu guggaucaga uuaggggcua ccgggucgac     1020

gugaacaaua aacccgagau gcauacuuuu gagguguauc gcaagaugca gagccuugaa     1080

acaaucccug ucggggaguu cucuagagaa guucuggaug aguuggcuca cauacuaacc     1140

cugaacacug aaagggaggg aauugaggaa gccaucaaua caaagcucau ggacguguuc     1200

ucccaagauc agauccugga gcuuguacag uuucguaaaa acaauucaag ccuguucagu     1260

aaggguuggc acaacuuuuc uuugaaacuc augauggaac ugauuccaga guuauacgag     1320

accuccgagg aacaaauggc gauccugacg cggcuaggca agcagaaauu caaggaaacc     1380

agcaagcgaa caaaauacau ugacgagaag gaagugacug aggagauaua uaaucccguc     1440

guggccaaau cgguuagaca ggcaaucaag auuaucaacg aagcuaccaa aaaguacggg     1500

aucuuugaua auauugugau agagauggcc agggaaaaca acgaggaaga cgcuaagaaa     1560

gacuauauca agcgccagaa agccaaucau gaugagaaga acgcagccau ggagaaagcu     1620

gcguuccaau acaauggaaa gaaagaacuc ccugacucua uuuuucacgg ccauaaggag     1680

cuggcaacaa agauccggcu uuggcaccag cagggugaaa aauguuugua uacugggaag     1740

aacauuccaa ucuccgaucu gauacacaau caguacaaau augagaucga ccauauucuc     1800

ccgcugucac uuaguuucga ugacagccug uccaacaagg ucuuggugcu cgccaccgcu     1860

aaucaagaaa aaggccagag aacacccuuu caggcccugg auucuaugga cgaugcaugg     1920

agcuacaggg aguucaaguc auacguaaag gacaguaaau uacugggaaa caagaaaaag     1980

gauuaucuac ucaccgagga agacaucucc aaaauugagg uuaagcagaa guuuaucgaa     2040

cgcaaucugg uggauacucg auacagcucu cgggucgugc uuaacacguu gcaagacuuc     2100

uauaaagccc acaaguuuga cacaaccaua ucagucgugc guggccaguu cacuucucag     2160

cugagaagga aaugggggcu cgaaaagagc cgcgagaccu accaucacca ugcuguugau     2220

gcccugauua ucgcagcguc cucucaguua cggcugugga aaaagcaagc uaauccucuu     2280

auuucguaua aggaagacca guucguggau agcgagacag gagaaaucau auccuugacc     2340

gacgauaaau acaaggagcu cguauuuaaa gccccauaug cacacuucgu cgacacucug     2400

ucaaguaaga aauuugaaga uucuauccua uucagcuacc agguggacuc caaguacaac     2460

agaaaaauuu cagaugccac aaucuaugcu acgaggaagg uuaagcugga caaagauaag     2520

agcgaggaga ccuacgugcu cgguaaaauu aaggacaucu auucucaagc cggcuacgau     2580

gcauuuauaa aaauuuauaa uaaggacaag uccaaauucc ugauguacca caaggacccc     2640

cagacuuuug aaaaagucau cgaggaaauc cuucgaacau auccuaguaa ggaguucaac     2700

gagaaaaaua aggaagugcc augcaacccc uuugagaagu accgccagga aaacgggccg     2760

auucggaaau acagcaagaa aggaaauggc ccugagauca agucuuugaa auauuacgau     2820

aagaacauuc ccgaauccuu cauaaauauc accccaucaa acaguaagaa uaaaguaauu     2880

cugagacagu uaaacccuug gaggacagac guguauuuua aucaucaaac ugagaaguac     2940

gaacuccugg gucuuaaaua ugcugaucug caguucgaga agaaaaccgg gacguacagc     3000

aucaccauug acaaguauaa cgagaucaag gaaaaagagg gcguuggaga acagucggag     3060

uuuaaguuca cauuguacaa aaaugaucuc cugcuaauaa aggacacuaa aaacaaugag     3120

aagcagaucu uccguuuucu gucccgcacc augcccaacg ucaaacacua cguggaacuc     3180

aagccauaug auaaggccaa auucgacggc aaucaacccc ugauuacaau cuuugggaac     3240

guggcgaagg gaggucagug ucuuaaaggc uugaauaagu cuaacauuag caucuucaaa     3300

gucaagacug auguucuggg gaacaagcau uuuauaacca aagagggaga aaagccuaaa     3360

uuaaaguucu ga                                                         3372


<210>  2800
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2800
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

aauaaggaga ccggggaaau cauucacgug aacuccagaa ucuuccccgc ugcaacagcc      120

gauucuaaug uagagaggcg gggauuucgc cagggccgac guuuggguag aaggaaaaag      180

caucggucag cgcgcuuaaa cgaccuguuc gaagaguuug gguucauuac ugauuuuagu      240

gcugugccuc uaaaucucaa cccauacgcc cugagaguca aaggacuuag cgaagaacug      300

acgaaugagg aauuguucau cgcacucaag aacauaauca aaaggcgggg cauuuccuau      360

cuggacgaug ccucugagga cggcgaaacc gcuucgaaug aguacgggaa ggcaguggag      420

gaaaaccgca agcugcucgc cgauaaaaca ccgggacaaa uccaguuaga gcgauuugaa      480

aaguaugguc agguuagagg cgacuucacu guggucgaga auggggaaaa ccacaggcug      540

auuaaugugu uuucaaccag cgcuuacaaa aaggaggccg agcggauccu ucgucgccag      600

caagaguuca acguaagaau aagugaugag uuuauugaag cguauuugac aauccugacc      660

ggaaaaagga aguacuauca uggccccggu aaugagaagu cccggacuga cuacgggcga      720

uuccgcacag auggaaccac gcuagacaac auuuuuggca uccucaucgg caaaugcacu      780

uucuacccug augaauauag agcagccaag gcuucuuaca ccgcccagga guucaaccug      840

cuuaaugacc ugaacaauuu gacaguucca acugagacca aaaagcucag gcccgaacag      900

aaacggcaga uuguggagua ugcacgcaca gcuaagaccc uggggacgcc uacuuuacug      960

aaauacauag ccaagcucgu cgacggaagc aucgaugaca uuaaggguua uagaaucgau     1020

aaauccgaca agccagaaau gcacacauuu gaugcguaca ggaaaaugcg uacccuugag     1080

cugguggacg uggauauauu gucacgggaa acucuagacg aucuggccua uauucucaca     1140

cugaacaccg agagugaggg cauccuugaa gcacugaaua gcaagaugcc cgggacguuc     1200

accaaagagc aaaucgacga auugauucag uuucgaaaga agaacucugc ugucuucgga     1260

aaaggcuggc auaauuuuuc ccucaagcug augaacgagu uaaucccuga gcuguacgaa     1320

acaucagagg aacagaugac uauucuaacc agacucggua aacagaggag ccgcgagaua     1380

aguaagcgga caaaauacau cgaugaaaag gagcugacug acgagauuua uaauccaguu     1440

guggccaagu cuguaagaca agcuaucaaa aucauuaacc uugccaccaa gaaauacggg     1500

auauucgaca auaucgugau ugaaauggca agggagucca acgaagauga cgagaagaaa     1560

gccauccaga auguccagaa ggcuaacgaa gaugagaaga aagcggcaau ggagaaggcc     1620

gcugacuugu auaauggcaa aaaggaacug ccggauagca uuuuucacgg acacaaagag     1680

cucgccacaa agauccgccu guggcaucaa cagggcgaac gaugucuuua caccgggaaa     1740

aacauaucga uccacgaccu gauucauaac ccccaccagu augagaucga ucacauuuug     1800

ccucucucuc uguccuucga cgauggauua gcaaauaagg ugcugguucu agccacugcu     1860

aaccaggaaa agggucaacg gacgccauuu caggcccucg acucaaugga ugacgcaugg     1920

aguuacaucg aguucaaaca guaugugcgu aauagcaagu cccugucuaa caaaaagaaa     1980

gauuaccuuu ugacagagga agacauaagc aagauugagg ucaagcagaa auuuaucgaa     2040

agaaaucugg uggacaccag guacucaagu cgcguaguuc ucaacacucu gcaagaguuc     2100

uauaagacca augauuuuga cacaaaaauu uccguggucc ggggccaguu caccagccag     2160

uuaagaagga aguggaaaau cgagaagucu cgagauacuu accaucacca ugcgguggac     2220

gcucugauaa ucgccgcauc aagucagcuu cgcuugugga agaaacaaaa caauccccuc     2280

auuagcuauc gggaagggca gcuggucgau ccugagacag gagaaauccu aucccugacg     2340

gacgaugagu acaaggaacu cgugcugaga ccacccuaug acuacuucgu ugauacccuu     2400

aaaucuaagu cguuugagga cagcauuuug uucuccuauc agguggauuc aaaauacaac     2460

aggaagauca gugacgccac uauauacggc acacguaagg cucagcuggg uaaagauaag     2520

caagaggaaa ccuauguauu agggaaaauu aaggacaucu acucucagaa aggcuaugag     2580

gacuuuauca agcgcuacaa aaaggauaca acucaguucc ucauguauca caaggacccg     2640

caaaccuuug ccaaagucau ugaagagauc cugaagacgu acccugauaa agaacuuaau     2700

gagaagggaa aagagauucc cugcaaccca uucgaaaagu aucggcagga gaauggcccu     2760

auaagaaagu acagcaaaaa ggggaaagga cccgaaauca agucccugaa auacuaugac     2820

aacaaguugg guaaucauau ugauaucacc ccagugaacu cacagaacca gguugugcuc     2880

caaagccuga agcccuggag gacagacguc uacuuuaauc cucagacuuc uaaauaugag     2940

cuaaugggcc ugaaguacuc cgaucuccga uucgaaaaag ggaguggaag cuauggcauu     3000

ucuccagaga aguacaacaa agugaaggca aaggagggug uagacgaaga uuccgaguuu     3060

aaauucaccc uguauaagaa ugaccuuauc uugauaaaag auacagaaac uggggagcag     3120

cagcuguuuc gguacggcuc acgcaacgac accaguaagc acuacgugga guuaaaaccg     3180

uaugaaaagg cuaaguucga gggaaaucaa cagcucauga accugcuugg cacgguugcc     3240

aaagggggac agugucugaa gggcaucaau aaacccaacc uaagcauuua caaggucaaa     3300

acagacgugc ucgguaauaa guauuuuauc aaaaaggaag gggaucagcc ucaacugaac     3360

uucaagaaaa agauuuga                                                   3378


<210>  2801
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2801
augaccagca agcaggagaa ccugggcgcc cucacauucg ggcuugacau cggaauugcu       60

uccguggguu gggcaguccu gggcgaaaau agaauaaucg auuugggggu uaggugcuuu      120

gacaaagccg agacugcgaa ggaaggagag ucuuuaaacc uggcucggcg cauggcccga      180

cuacuccgua gaaggcugcg gcgcagagca uggaggcuua cgaaacuggc ccgguugcuc      240

aagcgcgaag gccugauugc ugaugcaaau cuguucaaaa acaagaucga gaagaauacc      300

cccgugccua aaucaaguug gcagcuccga guagacgccu uagauagaaa gcugaacgcu      360

gaggaauggg ccagggugau uuaccaccuu uguaaacauc gggguuuuca cuggaucagc      420

cgugcggagg aaaaggcagc cgaguccgac ucuaaagggg agucgggaaa ggucaagcag      480

ggcuuggcug gcacuaaaaa gcugaugcag gagaaaaauu aucgcacagc cgcagaaaug      540

gugcuagcug aguucccaga agcccagaga aacaagcaag gggauuacuc aaaagcgcuc      600

agcaggauac ugcuuggaga cgaacugaag accuuguuug agcaucagcg gaaacucgau      660

aguccguucg ccacagagaa gcuggaagca gcuaucuuag guaauggcga ccgaaagucc      720

gggcuguuuu gggcccagaa ccccgcucuc gccggaucug aucuucugaa aauguugggc      780

uauugcaccu ucgagaagac ugaauaccgc gcaccuaaag ccagcuuuac agcugagaga      840

cacguuuggc uaaccaggcu gaauaaccuc cggauugugg ucgacgguac gacucgccca      900

cugaaugaag cggagagaag gaucgcacuu cugaugcccu aucagcaagc cggggauuuc      960

accuacaagc aguugcguac agcucucacu aaagccggau uucugccuga guccuuccgg     1020

uauaccggcu uaucauacgc aaguggccag aaggccgggg aaaagacaaa agacccagag     1080

agcgcugccc uggugaaguu ucccgcaugg caggaacuac gaaaaacccu cgagaagaaa     1140

gaacuggcgu cugaguggca aaagauuucc acggacgcuc uugauggaaa gccucaguug     1200

cuggaccaga ucgccugggu acucucaguu uacaaagaug acgaugaggu gaaggcagaa     1260

cugagcaaac uuaaccugcc aaaugccgag aagaugauag acgcuuugcu cgauauuaga     1320

uucgacaaau ucaguaaccu gucuuuaaag gcccugugua agaucguccc gcuaauggaa     1380

caaggucaga gguaugauga ggcaauugcu aacauccccg aguacggcca ucacucccag     1440

cuccacaaag ugggggccgg agaacauaag uaucugccuc cauuuuacaa agagcgcgac     1500

aagacuggcc ggaugguguu caaugaugac auggacaucc ccagaaaccc ugucguucuu     1560

agggcguuga aucaggcccg caaaguggua aacgcucuga uuaagaaaua ugauagccca     1620

cacgaagugc auauagagau ggcacgagac cucucgcggc cccuggauga acguagagag     1680

gucgagaagu uacaaaagga auuuagggac aauaacgaga aagguaaggc ccaguucgcu     1740

caggauuuua auaucguggg ggccguuaaa ggcaaggagu ucgaaaaaua ccagcuguau     1800

cgcgaacaac agggaaagug cgcauacucu auugagccga uggacaucca ccggcuuuuu     1860

gagcagggcu acguggaaau agaucacgcg uugccuuauu ccagaucauu cgacgauagu     1920

aagaacaaua ggguccucgu gcuggccaaa gagaaccgag acaaggggaa ucgcacaccc     1980

uuugaauacc uaggagguaa agauaacagc gagccauggc ggcaguucgu agcuuuuguu     2040

gaaggcaaua aggccuauag acuggcaaaa aggucccguc uccugcgcaa ggacuucggg     2100

gauaaggagg cuaaagaguu ucgggaaaga aaccuuaaug acaccaggua cauuuguaag     2160

uucuucaaaa acuaugugga gcaauacuug cagcugcaug aagacucuga ggccaagcga     2220

ugcgucgugu uaagcggaca gaugacuuca uuucuccggg cacgcugggg ccuggucaaa     2280

gugagaggug auagugacag gcaccaugcc cuugaugcug cgguuguggc cgcauguucc     2340

cacgggaugg uaaagcggcu gagcgacuau ucucgaagaa aggaguugga acagguccgc     2400

gagggcuucg uggauguuga cacaggagaa aucgugaacc cugcuauguu ucaacagguc     2460

caggagcauu uccccaaucc auggccccac uuuagggaug aagugaaacu ccgucuguca     2520

auggacgauc cugcccuacu gcggagugag auggagaagu ucggcaccua cacgccagaa     2580

gcccuccaag cucugcaccc gcuuuuugua agcagagcac cccagaggcg caacuccggg     2640

gccgcgcaua aagacaccau cuacgcucag ccuaagcgau ugguggccca gggagguguu     2700

acacaaaaag ucgcacugac uucuuuaacc cucaaggauc uggacaaacu uauugaucca     2760

cacagaaaug acaagcugua uacagccauc cgggcuaggc uagagcagca uggcgggaag     2820

ggagacaaag cauuccccuc gagcaacccu cuccgcaagc cagauuccga aggcaauccc     2880

acugguccga uugugcgugg ggugaccaug gucauagaca aacugucagg caucccuauu     2940

agaggaggca ucgccaagaa cgauaguaug guucgggugg acguguuuac aaaagcgaau     3000

aaguuccacu ugguaccagu cuacgugcac cauaccguug cuaaggcccu gcccaacagg     3060

gcaguggucg ccuuuaaaga ugaggacgaa uggacuuuaa ucgaugacuc uuucgauuuu     3120

ugcuucagcc uccacccuaa ugaccuggug cagguucagc aaaaggggaa agcuggaaug     3180

auuggcuauu acuccucaug ucaucgcggu acggggaaca uaaaucuuug ggcccacgau     3240

cgaaaccugc aagugggaaa ggacggcaug aucgagggua uuggcguaaa aacagcauug     3300

agccucgaga aguucaaugu cgacgugcug gggaacagau aucccgcucc accugaaaag     3360

cggagggauc uagccuga                                                   3378


<210>  2802
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2802
augaccgaca gaaucagccu gggccucgau auuggggugg ccuccgucgg auucucuguu       60

cuugacauag auaaggguaa agugaucgag cugggcgcua gguuguuuaa cgcaacagua      120

gccgcgggga aucaggaccg gcgcgauaug cgaggaucac guagauuacu gaacaggaau      180

aagcaacggc gcaaagacgu gaguaagcua uuccaggaau acggccuccu ggauaacuuu      240

gacagagaua auuuccacac ugcuuuuaac aauaacugga aucccuauga gcuuaggguc      300

aaaggucuga cgaagcaguu gaacaaggaa gaacucgccg acagccugua ccagauuauc      360

aaacggcgcg ggauuuccua ugcacugaag gaugccgacg cuaaugaaga uggaaccgac      420

uacucuucgu cacucaaaau caacagccaa gaguuaauag agaagacucc ugcacagauc      480

cagcugcagc gacuuaauga cuauggcaaa gugagaggca agguuauugu gggggaugac      540

aucgaugacc aaaaggucuu gcugaacgug uucccaacaa augccuacga aaaagaggcu      600

aggcagauua ucgccaccca gcagcaauuu uauccggaaa uacuaacagg agaguucgua      660

aaggaguacu gccagauucu cacccggaaa cgugauuacu uuguuggucc cggcaacgaa      720

aagagucgca cugacuaugg gaucuacaaa acagauggaa gaacccugga caaccuuuuc      780

gaggaacuga uuggccauga uaagaucuau ccugacgagu ugagggcguc cgcagccucu      840

uacacggcuc agcucuuuaa ugugcugaac gauuuaaaua accugcggau ccucagcuau      900

gaagacggua aacuuacuca aauugacaag gagaagauaa ucgccgagau uaaaaauaac      960

accacagucg ugaauaugcu gaacgugauc aagaaaguca cuggguguca gaaggaugac     1020

auaaaaggau uccgauugaa ugaaaaggau aagccagaga uuuccucaau gcccaucuac     1080

cgcaaagcac acaaggacuu ucuaaaaguu ggcaucgaua uuaccgacug gccuaucgau     1140

uucauugaca gacugaguuu cauacucaca cugaacaccg aaaauggcga gaucaggaag     1200

cagauuaaca aacggcuugu gccagauuuu gacuuccuga augaugacuu gguacagcuc     1260

aucaucgaua acaaggacag cuuugacauu aagacgaaua acaaauggca ucgcuucucu     1320

cugaagacua ugaacaaauu aauacccaca augaucgaaa gaccugugga gcaaaugacc     1380

cugcuaacug agauggggcu cauuaagaaa gauacaaaga gguuugaagg aaauaaguau     1440

cugccauaca aagagaucgc uaacgacauu uucaauccgg ucgccuccaa gucagugcgu     1500

gaagcgcuua aaaucguuaa cgccguguug aagaaauacg gucacauaga guauaucguc     1560

auugaaaugc cccgggauaa gaaucuggac gaggagcgaa aacagaucgc agauuuucag     1620

aagaagaaca aaaagauuaa agacgcugcc uucaaugcuu uugugaaggc cguaggcagc     1680

aaaaagaacg uuaagaucgc acucaguaaa aauagaaagc ugcagauggg gauaaggcuu     1740

uggcaucaac agcagggaau ugauccuuac aacggcgaag agaucaaugc caacgaccug     1800

auuucuaauc cagauaaauu cgaaaucgac cacauaaucc cccaguccau uagcuuugau     1860

gacgguauca acaauaagac cuugugcuuc gcuucgauga accaagugaa agggcagaag     1920

acgccuuaug aguuucucaa cgagggccac ggacagggcu acgcgaaguu caaagcaauu     1980

gucucuaaga auaaaaacuu cuccaaggcc aaacgcgaua auuaucuguu ugaagagaac     2040

gugucaaaua ucgaaacccg gaagagauuc uuaaguagga accuggucga cacacgcuac     2100

agcucccgag ugguucuaaa uucucuccag gaguuuuucc augaaaagag cgacgauacu     2160

aaagugaccg uaauacgggg gaaguuuaca ucaaacaugc guaaacacug gcauauugac     2220

aagacuagag auaccuucca ccaccaugcu aucgacgcca guaucauugc agccacacca     2280

uuucugagga uguggaaaaa gggauccacc aucuucccca cuaaaauuaa ugagacgagc     2340

auagauaucg agacagguga aauucuugac gauaagaacu uugacaaggu cauguaugag     2400

gaaccguacu cuggcuucau cucagagauu augaaugcug augaccgcau caaauuuagu     2460

caccaagugg auaagaaaau gaaccggaag guuagcgacg ccaccauaua cuccacuaga     2520

aucgggaaau ugucuaagga caagaaagau gcagaauaug uggucgcgaa ggugaaagac     2580

auuuacucgg uagauggaua ugagaauuuc aagaaagugu acaagaagga caaaaccaag     2640

uuucugcucu auaaauacga uccuaggaca uucagcgagc uggaaaagau cguuauugac     2700

uaucccgaua aaaucgagaa gguccagacc aacggcaaga uaaaagcugu ggacauuucc     2760

ccauucgaau uauaccgacg cgauaauggu auggugcgga aguacaguaa aaagacuggg     2820

ccugccauca aacagcugaa guaucuugac aagaaauugg gcucacauau cgauauuaca     2880

cccaagaacg caaacaauaa acacgucguu cuccaaucuc ugaagccaug gagaacggac     2940

guguaccuaa accaugagac cggagaauau gagauaaugg gcaucaaaua cagcgaucug     3000

aaguuuaaua aaaacgaggg guauggaauu aagaaggaca aauaccucaa gaucaaaaau     3060

auugaaggug uguccgagaa cucagaguuc auguuuagcc uguauaggaa ggaccguauc     3120

aaaguacagg auauggagac uaaugagucu gucgaacuuu uguucugguc ccgcaacuuu     3180

aguaauaaga aguacgccga gcugaaaccc auaagccaga ucgacaacga uaagguguua     3240

ccuauuuacg gccgggggag acucaucaaa aggcugauuc caaagaauug uaaaaucugg     3300

aagguuaaca caucuauacu uggagacacc uauuaccugg aaaaggaguc caauuauccg     3360

cagaaaauuu uggauuga                                                   3378


<210>  2803
<211>  3381
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2803
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

aauaaggaga ccggggaaau cauucacgcu aacuccagaa ucuuccccgc agccacagcg      120

gauucuaaug uggagaggcg gggauuucgc cagggccgac guuuggguag aaggaaaaag      180

caucggucag cucgcuuaaa caaucuguuc gaagaguuug gguucauuac ugacuuuagu      240

gccaucccuc uaaaccucaa uccauacgca cugagaguaa aaggacuuag cgaagagcug      300

acgaacgagg aauuguucau agcccucaag aauaucauua aaaggcgggg caucuccuau      360

cuggaugacg cuucugagga uggcgaaacc gcaucgaacg aguacgggaa ggccguggag      420

gaaaaucgca agcugcucgc ugacaaaaca ccgggacaaa uucaguuaga gcgauuugaa      480

aaguaugguc aggucagagg cgauuucacu gugguugaga acggggaaaa ucacaggcug      540

aucaacgugu uuucaaccag cgccuacaaa aaggaggcgg agcggauacu ucgucgccag      600

caagaauuca augucagaau uagugacgag uuuaucgaag cauauuugac aauucugacc      660

ggaaaaagga aguacuauca uggccccggu aacgagaagu cccggacuga uuacgggcga      720

uuccgcacag acggaaccac gcuagauaac aucuuuggca uccucauugg caaaugcacu      780

uucuacccug acgaauauag agccgcuaag gccucuuaca ccgcacagga guucaaucug      840

cuuaacgauc ugaauaacuu gacagugcca acugagacca aaaagcucag ccccgaacag      900

aaaaggcaga uaguagagua ugcucggaca gccaagaccc uggggacgcc uacuuuacug      960

aaauacaucg cgaagcucgu ugacggaucc auugacgaua ucaaggguua ucgcauagac     1020

aaaucagaua agccagaaau gaugcacaca uuugacgccu acagaaaaau gaggacccuu     1080

gaucugguga auauugacgc auugagucgu gagacucuag augaccuggc ucauauccuc     1140

acacugaaca ccgaaagcga gggcauccuu gaggcccuga auucuaagau gcccuccacg     1200

uucaccaaag aacaaauuga ugaguugauc caguuucgga agaagaacuc agcugucuuc     1260

gggaaaggau ggcacaauuu uagccucaag cugaugaacg aauuaauuag ugagcuguau     1320

gagacaucug aagagcagau gacuauacua acccgacucg gcaaacagag auccagggaa     1380

aucagcaagc gcacaaaaua cauugacgag aaggaacuga cugaggagau cuacaauccu     1440

gugguggcca agucgguccg gcaagcaauc aaaauuauaa acgaagccac caagagauau     1500

gguaucuucg auaauauugu uaucgagaug gcuagggaaa acaaugagga agacgcgaaa     1560

aaggacuaca uuaaacgcca gaaggcaaac caggaugaga agaacgccuc uauggagaaa     1620

gcugccuuuc aauauaaugg gaagaaagaa cuuccagacu ccaucuucca cggccauaag     1680

gaguuggcaa caaaaauacg acuguggcac cagcagggag aacggugucu cuacaccggc     1740

aagaacaucu caauucguga ucugauccau aauccgcacc aguaugagau ugaccacauc     1800

cuuccccuga guuugagcuu ugaugacggg cucgccaaca aagugcuggu auuagcuacu     1860

gccaaucaag aaaagggaca gagaacgccu uuccaggcaa uagauuccau ggacgaugcg     1920

uggucuuaca gggaguuuaa gcaguaugug cgcaacagca aaucacugag uaauaagaaa     1980

aaggacuacc uacucacaga ggaagauauu uccaaaaucg aggucaagca aaaguucauu     2040

gaacggaacc ugguggacac cagauacagc ucuaggguug ugcuuaauac uuugcaggag     2100

uuuuauaaaa ccaacgauuu cgacacaaag aucucagucg ugcgagguca guucaccagu     2160

cagcugcgcc ggaaauggaa gauagagaaa agcagagaca cuuaccauca ccaugcugua     2220

gaugcccuca ucauugcagc cuccucucaa cugagguuau ggaagaagca gaauaaccca     2280

cugaucucgu auaaagaagg ccaguuuguu gaccccgaga caggggaaau ucuuagcuug     2340

acggaugacg aguacaagga acucguguuc cguccuccau augauuacuu ugucgacacc     2400

cugaaaucca agucauucga ggauaguauc cuauuuucuu aucaggugga cagcaaauac     2460

aaucgcaaga uauccgaugc uacuauuuac ggaacacgga aggcccaacu gggcaaagac     2520

aagcaggagg aaaccuaugu ccucgguaaa aucaaggaua ucuacucaca gaaaggguau     2580

gaggacuuca uuaagagaua caacaaagau gaaacacaau uucugaucua ucacaaggac     2640

ccccagacuu ucgagaaggu gauugaagag auacuuaaaa ccuacccgga caaggaguug     2700

aaugaaaaag gcaaggagau cccuugcaac cccuuugaaa aauauaggca ggagaacgga     2760

ccaauucgaa aguacagcaa gaaaggcaag gggccugaaa ucaaaauucu gaaguacuau     2820

gauaauaaau uaggaaacca uaucgacaua acgcccguua auucucagaa ccaaguggua     2880

cuccaguccc ugaagccaug gcggaccgau gucuacuuca auccccgcac aaguaaguau     2940

gagcuuaugg gucugaaaua cagcgacuug agauuugaga agggcucugg guccuaugga     3000

aucucaccug aaaaauacaa caaggugaaa gcaaaggagg gcguuaauga agauagugag     3060

uucaaguuua cucucuauaa aaacgaccug auucuaauca aggauaccga gacaggugaa     3120

cagcagcugu ucagguacgg gagccggaau gacacuucga aacacuacgu ggagcucaag     3180

ccauaugaaa aagcuaaguu cgagggcaac caacagcuga ugaaucuuuu gggaaccguc     3240

gccaagggcg ggcagugucu gaaaggaauu aacaagccga auuuauccau cuacaaagug     3300

aagacggaug uacucggcaa caaauauuuu auaaagaaag aaggugacca gccccaacug     3360

aauuucaaga agaaauuuug a                                               3381


<210>  2804
<211>  3381
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2804
auggccagca agcacgagaa ccugggcgcu cucaccuucg ggcuugacau cggaauugca       60

uccguggguu gggccguccu gggcgaaaau agaauaaucg auuugggggu uaggugcuuu      120

gacaaagcgg agacagcuaa ggaaggagag ucuuuaaacc uggcccggcg cacugcacga      180

cuacuccgua gaaggcugcg gcgcagagcc uggaggcuua cgaaacuggc ucgguugcuc      240

aagcgcgaag gccugauugc agauaccaau guguuccaga aacccuuuaa ccugaaggac      300

gccaaugaga agggucucuc auucuggcag uuacgaguag augcucugga cagaaaacuu      360

agugccgagg aaugggcgag ggugaucuac cauuugugua agcaccgggg guuucauugg      420

auuagccgug cagaggaaaa agccgcugag ggagauucca agucugaggg cggcaaaguc      480

aagcaggggc uggccggaac aaagaaacua augcaggaaa agaacuaucg cacugcagcu      540

gagauggugc ucaccgaguu cccugaagcc cagagaaaua aacaagguga cuacucgaag      600

gcgcugucaa ggauccuucu gggcgaugaa augacacagu uguuuaaaca ccagcgggag      660

cucgggaacc cacaugccac cgagaagcug gaagcagcua uauuaggaaa uggcgaccac      720

aaaagcgguc uguucugggc ccagaacccg gcucucgccg ggaaugaucu ucugaagaug      780

uugggacacu gcacuuuuga gaaggcagaa uaucgaguuc ccaaagccag uuucacagcu      840

gagcgccaug uguggcuaac cagacugaac aaucucagga ucgucgugga cggcacgacu      900

cggccucuga acgaagcgga gcgccaccuu gcacuguuga ugccauacca acaggccggc      960

gaucucaccu auaagcagcu gagaacagcu uuaacuaaag ccggguaccu gcccgagucc     1020

uuuagguaua ccggacuagc auacgccucu ggucagaagg cugacgaaaa agccaaggac     1080

ccugagagcg caccacucgu aaagcugccc gcguggcaug aacuucguaa aacauuggag     1140

aagaaagaac uggcuuccga guggcaaaag auuucaagug augcccucga cggcaaaccu     1200

cagcugcuug aucagaucgc auggguucug agcguguaca aggacgauac cgaggucgaa     1260

gccgaguugu guaagcucaa ucugccaaac gcugagaaaa ugauugacgc cuuacuggau     1320

auccgguucg acaaguuuuc uaaccuaucc cucaaagcac ugcgacaaau agugccgcuu     1380

auggaacagg ggcagagaua ugaugaggcu guggccaaua uucccgaaua cggacaccac     1440

ucacaguugc augaggucgg cgcgggugaa cacaaguauc ugccuccauu cuacaaagag     1500

agggacaaga cggggcgcau gguuuucaac gaugacaugg acaucccccg gaauccugug     1560

guacucagag cccugaacca agcuaggaag guggucaaug cauuaauuaa agaauauggc     1620

agcccacaug aggugcacau cgagauggcc cgcgaucuga gucgaccccu ugacgaacgg     1680

cgugauguug agaaguugca gaaagaauuu agagacagga acgagaagga uaaagcucuc     1740

uucgcccagg acuuuaauau cgugggagca gucaagggca aagaguucga aaaguaccag     1800

cuguaucgcg agcaacaggg gaagugcgcg uacucuauug aaccgaugga uauacaucgg     1860

cuauuugagc agggauacgu ggaaaucgac cacgcccugc cuuauuccag gagcuucgau     1920

gacucgaaaa acaauagagu acucguucug gcuaaggaga accgagauaa agguaaucgc     1980

acucccuuug aauaccuugg cgggaaggac aacucugagc gguggagaca guucguggcc     2040

uuugucgagg gaaauaaauc cuauagguug gcaaagcguu cacgccuguu acggaaggau     2100

uucggcaaag acgaagcuaa ggguuuuaga gagaggaacc ucaaugacac acgauacauu     2160

ugucgguucu uuaaaaacua uguggaacaa uaccugcagc uucacgagga uagugacgcc     2220

aagcgcugcg ucgugcugag cgggcagaug accuccuucu ugagagcaag guggggccuc     2280

guuaaagugc ggggagauuc ugaccgacau cacgcccugg auggcgcuau cguagcggcc     2340

uguagccauu caauggucaa gagacuaagu gacuauuccc gcaggaagga gcuggaacag     2400

gugcgugcag gguucguuga uguggacacu ggagagauag ucaacccagc uauguuucaa     2460

caggugcagg aacacuuccc uaaucccugg ccacacuuuc gggaugaggu aaaacucaga     2520

cugaacgugg acgaucccgc ccuuuugcau agcgaaauga auagguucgg uacauacucu     2580

ccugaggccc ugaaggcuuu acgcccacuc uuuauuucac gagcaccgca aagacggaac     2640

aguggcgccg cgcacaaaga caccaucuac gcucagcccg agaggcuggu ugcccaaggg     2700

ggagucacgc agaaggugcc ucuuaccagc cugacacuaa aagaucucga caagcugauc     2760

gacccacauc gcaaugaaaa guuguauucc gcaauucgua ucagacugga gcagcacggc     2820

gguaaagggg auaaggccuu ccccgcuucu aacccuuuac ggaaaccaga cucggaaggc     2880

aaucccacug gaccgauugu gaggggcguc accaugguua uagauaagcu cagcgggauc     2940

ccuauucgcg gaggcaucgc caaaaacgac uccauggugc gaguggaugu auuuacaaag     3000

gcagguaaau uccaucuggu cccaguguac guucaccacg cgguggcuaa gguccuuccc     3060

aauagagccg ugguugcaua uaaggacgag acugaaugga cccugaucga ugacggguuu     3120

gauuucugcu uuucaaugua cccuaacgac uugcuccgga uuaaacugag gaaugccagu     3180

caucuaggau auuacgcuuc uuguaaccgc agcacaggcg ccauaaaucu guggucccac     3240

gauagaaacc aacagguggg uaaggacggc augaucgagu caauuggggu aaaaaccgca     3300

cuuaaugucg agaagcucaa caucgacgug cugggaaaca gguaucccgc uccaccugaa     3360

aaacggcgug auuuggccug a                                               3381


<210>  2805
<211>  3384
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2805
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaaug uagagaggcg gauuaaccgc caaggacgac guuugacaag aaggaagaaa      180

caucggcgcg ugagauuaaa ucaccuguuu gaagagucag gccuaaucac ugacuucacg      240

aacgucagua uaaaucucaa cccuuaccag cugaggguga agggucuuac cgaugaacug      300

agcaaugagg aguuguuuau cgcccucaaa aacaugguua agcaucgggg gauuuccuau      360

cuggacgaug cgucugacga uggaaauucg ucagugggcg acuacgcuca gaucgucaag      420

gaaaacagca aacagcugga gacaaagacu ccaggccaaa uucagcucga acgcuaucag      480

aaauacgggc aguuacgagg agauuucacc guggaggagg acgguaagaa acacagacug      540

aucaauguau uuccgacaag ugccuauagg gcagaagccc uucggauauu gcaaacccag      600

caggaguuca acccccagau uacugaugaa uuuaucaauu ccuaccugca aauucuaaca      660

ggcaagcgua aguauuacca ugggccugga aacgagaaau cucgcaccga cuacggcaga      720

uauacgacua agaaagauag cgaagacgag uacaucaccc ucgacaacau cuucgguauu      780

cugauaggga agugcacauu uuauccagag gaauacaggg cugcaaaagc cuccuauacu      840

gcucaggagu ucaaucuucu gaacgauuug aauaaccuca ccguucccac agaaaccaag      900

aaacugucag aggaacagaa gaaucaaauc auuacgaacg ugaagaauga gaaagccaug      960

ggaccugcga aguuauuuaa auacaucgca aagcugcuca guugugacgu cgccgauaua     1020

aaaggcuauc ggauugacaa gagcgauaag gcugagaucc acacuuucga agccuaccga     1080

aaaaugaaga cacuugcaac caucgacauu gagcagaugg aacgcgagaa ccuggauaaa     1140

uuggcuuacg ugcuaacucu gaauacagaa aaggagggca uccaggaggc ccucgaacac     1200

gaguuugcgg acgggaccuu cucucaggaa caaauugaug agcuggugca guucagaaaa     1260

gccaacuccu caauauuugg aaaggguugg cauagcuuca gugucaagcu gaugauggag     1320

cuuaucccag aauuguaugc aacgucugag gaacagauga ccauucucac aaggcugggc     1380

aaacagaaga cuaccuccag cucgaauaaa acaaaguaca ucgacgagaa acaauuaacu     1440

gaagagaucu auaaccccgu ugcugccaag ucugugcggc aggcuauuaa gauaguaaau     1500

gccgcaauca aagaguacgg ggauuuugac aacauuguga ucgaaauggc ccgcgagacc     1560

aaugaagacg augagaagaa agcuauucag aagauccaga aagcgaacaa ggacgaaaaa     1620

gaugcagcca ugcugaaggc ugccaaccaa uauaauggaa aggcagagcu accucacucc     1680

gucuuccaug gccacaaaca gcucgccaca aagauaagac uguggcacca gcagggugag     1740

aggugccuuu acaccgggaa aacuaucuca auucaugacu ugaucaacaa uaguaaccaa     1800

uuugaaauug aucacauccu gccacucagc auaacguucg acgauucccu ggcuaauaag     1860

gugcuuguuu augccacagc aaaccaggag aaaggccagc guaccccgua ccaagcgcug     1920

gacucuaugg augacgcuug gagcuuucgg gaauugaagg ccuucgugcg agauucaaag     1980

gcacucagua auaaaaagaa agaguaccug uuaacugaag aggacauuuc caaguuugau     2040

gucagaaaga aguucaucga gaggaaccug guggacaccc gcuaugccag ccggguaguu     2100

cuaaaugcuc uccaggaaca uuuuagagug cacaagacag acaccaaagu cucugugguc     2160

aggggacagu ucacuucaca gcugcgccga cauuggggca uugagaagac acgggauacg     2220

uaccaccacc augccgugga cgcacuuauc auagcugcca guagccaauu gaaccugugg     2280

aaaaagcaga aaaauacccu cguuuccuau ucugaagauc agcuguuaga caucgagacu     2340

ggggagcuga uuucggauga cgaauacaag gagagcgugu uuaaggcgcc cuaucagcac     2400

uucguagaua cacuuaaauc caaggaguuc gaagacucaa ucuuguuuag uuaccaaguc     2460

gauucuaaau ucaaccguaa gauuagcgac gccaccaucu augcuacaag acaggcaaaa     2520

gugggaaagg auaaaaagga cgaaacuuac guucucggua agauaaaaga uauuuacucc     2580

cagaccggcu augacgccuu uaucaagauc uacaaaaagg acaaaucaaa guuccugaug     2640

uauaggcaug auccucagac guuugagaag gugauugagc caauccuaga aaauuacccc     2700

aacaaagagc ugaaugaaaa ggggaaagag gucccuugua acccauuccu caaguauaaa     2760

gaagaccacg gauacauucg caaguauagc aagaaaggca acggucccga gauaaagucu     2820

cugaaauacu acgauuccaa guuggggaau cacaucgaca uuaccccgaa aaacaguaau     2880

aacaaggugg uacuucaaag cgugucuccu uggcgggcug auguuuauuu uaauaagaca     2940

acuggcaaau acgagauccu gggauuaaag uaugccgacc ucaaauucga aaagggcacc     3000

gggacauaca aaauuuccga ggaaaaguau aacgauauca agauaaaaga gggagucgac     3060

ucagauagug aauuuaaguu cacucuguac aaaaaugacc uucuguugau caaggauacc     3120

gagacgaaag agcagcagcu cuuuagauuc cugagcagga caaugcccaa cgugaagcau     3180

uauguggaac uaaaaccaua cgacaagcaa aaguuugacg auaaugagga acugauuaaa     3240

auccucggua uugucgcaaa gggcgggcag ugcaaaaagg gaguuucgaa accuaacauc     3300

uccauauaca agauucgaac cgacgugcug ggcaaucagc acaucaucaa gaacgagggu     3360

gauaaaccca aacuugacuu cuga                                            3384


<210>  2806
<211>  3384
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2806
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaggauu ccgggaaaau cauucacgcu aauucuagaa ucuuccccgc agccaccgcg      120

gacaacaaug uggaaaggcg gacaucacgc caggcucgac guuugaacag aaggaagaaa      180

caucggagug uacgcuuaca cgaucuguuu gaggaauacg gacuacucac ugacuucagc      240

aaggugucca uuaaucugaa cccuuaucaa aucagagucc agggccuuga uaaaaggcug      300

acgaaugagg aauuguuuau agcccucaag aacaucguga agcggcgcgg uauuucuuac      360

cuggacgaug caucggagga cgggggagcc guuucaagcg auuauggcaa agcuguggag      420

gaaaaucgaa agcugcucgc acagcagacc ccaggccaaa uccaguuaga cagauucgag      480

aaauacgggc aggucagggg agauuuuaca gugguagaaa acggugagaa gcggcgucug      540

auuaauguuu ucaguacuuc cgccuaucgc aaagaggcug aaagaauccu uaggaagcag      600

caagaguuua acaauaagau aaccgaaggc uucauugagg acuacuugac aauccugacc      660

gggaaacgga aguauuacca uggaccgggc aacgaaaaau cucgaacuga uuacggucgc      720

uauacaacca agaaagacag ccagggggag uacauuacgc uagacaauau cuuuggaauc      780

cucauuggca agugcacuuu cuaucccgau gaguacagag ccuccaaagc gucauauacc      840

gcacaggaau uuaaccugcu uaacgaccug aauaacuuga cagugccuac ugagaccaag      900

aagcucagug ucgaacagaa aaagacaaua guggaguacg ccaaaaccgc uaagacgcug      960

ggcgccagca cuuuacugaa auauaucgca aagcucauug gggugucugu cgaucaaauc     1020

cacggauaca ggauagaccc aaaugaaaag cccgagaugc auacauucga gguuuaccgg     1080

aaaaugcagu cccuugaaac cauucgcgug gaggaacugc cuagaaaggu aaucgaugag     1140

uuggcucaca uccuaacucu gaacacagaa agggagggua uugaggaagc caucaaugcg     1200

aaacucaagg acauauuuuc acaggaucaa gugcuggagc uuguccaguu ccguaaaaac     1260

aauagcaguc uguucucuaa gggcuggcac aacuuuuccu ugaagcucau gauggaacug     1320

auuccagagu uauaugagac cagcgaagaa cagaugacga uccugacccg gcuagggaaa     1380

cagaagucga aagaaacauc uaagcgaacu aaauacauug acgagaagga acucaccgag     1440

gagaucuaua aucccguggu ugccaagucc gugagacaag caaucaaaau uauaaacgaa     1500

gcuacaaaga gguacggaau cuucgauaau auugucaucg agauggcccg cgaaaacaau     1560

gaggaagacg cuaaaaagga guauauuaaa cggcagaagg ccaaccugga ugagaaaaau     1620

gcagccaugg aaaaggcugc guuucaguac aacggcaaga aagagcuucc ugacaacgug     1680

uuccaugguc acaaggaauu ggcaacuaaa aucagacugu ggcaucagca aggggagaag     1740

ugucucuaua ccggcaaaaa uauaccaauc ucagaccuga uucagaacag guacaaguac     1800

gaaaucgauc acauucuucc gcugaguuug agcuuugacg auucccucuc uaauaaggua     1860

cugguuuuag ccacagcuaa ccaggagaaa ggacagcgca cccccuucca agcccuggac     1920

agcauggauu augcaugguc auaccgagag uuuaagaguu augugaaaga cuccaagcua     1980

cucagcaaua aaaagaagga uuaccugcuu acugaagagg acaucucuaa aauagaaguc     2040

aagcagaaau ucauugagcg gaacuuggug gauacgcguu auucaaguag agucgugcug     2100

aaugcccucc aggacuuuua caaggagcac caguucgaua caaccaucag cguugugagg     2160

ggccaauuua cuucccagcu gcgccggaaa ugggggauug aaaagucuag agagaccuau     2220

caucaccaug cuguagacgc cuuaaucaua gcagcgucga gccagcugag gcuuuggaag     2280

aaacacaaca auccuuugau cuccuacaag gaggagcaau ucgucgauuc agaaacagga     2340

gagauuauca guaugucuga cgacgaguac aaagaacucg uguuuaaggc uccauaugau     2400

cauuucguug acacccugaa aagcaagaaa uucgaggauu ccauucuauu uucauaccag     2460

guggacagca aguauaaccg aaagaucucu gaugccacua uauacgcaac acgcaaagcc     2520

aagcuggaca aagauaaguc cgaagagacg uauguccucg guaaaauuaa ggacaucuac     2580

agucaggcug gcuaugauac cuucaucaag auuuacaaua aagacaagag ccgguuucug     2640

auguaccaca aagaucccca gacuuucgaa aaggugaucg aggagauucu uagaacauau     2700

ccuucuaaag aauugaacga gaagaauaag gaaguaccau gcaaccccuu ugagaaauac     2760

aggcaagaaa augggccgau acguaaguau ucccgcaaag gaaacggccc ugagaucaag     2820

ucacugaaau acuaugacaa caaguuaggu aaucacauug auaucacccc caacgagagu     2880

augaaucagg ugguucucca gagccugaag ccauggcgga cagacgucuu cuuuaaccau     2940

cagacugaaa aauacgagcu uauggggcug aaguauucgg acuugaauuu cgaaaaaggc     3000

uccggaaagu acucuauuag ccucgagaaa uacaacucaa ucaagaugaa ugagggcgug     3060

agugaagagu ccgaauuuaa guucacccug uauaaaaacg aucuaauacu gaucaaggac     3120

gcccucacga aagaggcaca aauuuuuaga uacgggucua ggaaugauac cagcaagcac     3180

uauguggaac ugaaaccuua cgacaugucc aaguucgagg gagaugagcg acuuaucaaa     3240

gucuugggua caguugcuaa gggcgggcgg uguauuaagg gacugaacaa aaaggacauc     3300

ucaauauuua aagugaagac ugauguguua ggcaauaaac auuucauuaa gaaggaaggu     3360

gaggaaccca aacucaaguu cuga                                            3384


<210>  2807
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2807
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uugguagcaa cuccauaggc       60

uucgccgcug ucgaugaaaa uucucaccuc aucagacuua aggggaaaac aguuauugga      120

gcaaggcugu uugaagaggg caaggccgcg gcuaaacggc gcgccggucg aacuacgcgu      180

agaagguugu cacggaaccg cuggagauua aguuuccuga gggacuuuuu cgaaagccau      240

aucaccccua cagauccaaa cuuuuucaug cggcagaagu auuccgagau uucuccgaag      300

gacaaagcac gcuacaagua ugaaaaacga cuauuuaaug auagaacuga cgccgaguuc      360

uaccaacagu auucgaccau guaccaccuc aggaaucggc ugaugacaga ucccucacgu      420

gcugacgugc gcgagaucua uuuugcaaua caucacaucc uuaagagcag agggcauuuc      480

cugaccccug gagaugccaa agacuuuaac acuaaugaug uagcuuugga cgaaauuuuc      540

ccagcccucc aggaugcgua cgcacaggug uaccccgacc ugggcaucac auuugacgag      600

gauaaggcca acgaguucaa gaccauucug cucaaugaga ucgcuacgag uauagacacu      660

caaagggccu uagucaaacu gcuuuuggca gaggaagaug acaaggauau ucugaaacag      720

cagaaacagg ugcuaaccga guucgcuaaa gccguugugg gccucaagac aaaacugaac      780

cuugcgcugg ggacugaagu cgacuccucu gaggccaccg cauggaauuu uagcuuggga      840

caacucgaug acaagugggc ugguaucgaa uccgccauga cagaugaggg caccgagauu      900

cuggaccaga uccgggauuu auaucgagcu cgccugcuca acgggaucgu gccuacggga      960

aagacucuuu cacaggccaa aguagacgac uacacacagc accacgaaga ucugcaauug     1020

uucaaggcau aucuaaaaca gcuggaggac gauggcaccg ccaaggcuau uagacagcuc     1080

uacgacaggu auauagaugg ugacgaugcg gcaccauuuc ugcgggaaaa uuucguuaaa     1140

gcccuuacua aggacgugac agcucauccc aacaccaaga guccugagcu guuggaacgc     1200

cucgcccaac cagauuuuau gcugaaacag agaaauaagg caaacggggc caucccgguc     1260

cagaugcagc aaagggaguu agaccagauu aucaaaaacc aggcugugua cuaugauugg     1320

cuggccgcac ccaauccugu ggagaagcac cguaaaagca ugccauacca gcuagacgaa     1380

cuccugaacu uccggauacc cuacuauguc ggaccucuug uuacggcgaa ggagcaaaag     1440

gcugcccagg gcggcguguu ugcauggaug guacgaaaag auccagaagg gaauauuacc     1500

cccuacaacu ucgacgagaa gguggacaga gaagccucug cuaauacauu uauccagagg     1560

augaaaacua ccgauacaua uuugaucgga gaggacgucc ugccgaagca gucccuccug     1620

uaccaacgcu augaggugcu uaacgaacug aauaacguuc gggugaauaa cgagaaauug     1680

ucaauugaac agaagcagca ggucaucaga gagcuuuucg agaggcauaa uagcgugacu     1740

auuaagcaau uugccgaaaa ccugcgcgca cacggugauu acgcucacau accugagauc     1800

cgaggcuuag ccgacgaaaa acgguuccug aguucucuau ccaccuaucg ucagcucaag     1860

agccugcuuc ccgaggcgau ugaugaccca gccaaacagg cugauaucga aaauaucauu     1920

gcauggucga caguauuuga ggacgccgcu auauucaaga ccaaauugaa ggagaucucu     1980

uggcuggggu ccgaagccau uacuaaacuc ucaaacauca gauacagggg auggggccaa     2040

uucagucgca aguuucugaa ugguuuaacg cuggggaacg gccauacaau uauccaggag     2100

cuuuugcuca gcaccaacaa ucugaugcag auacuaacug augaaacccu gcagaagaaa     2160

augacagagc ucaacgcaga caagcugaaa accgcgaaua ucaacgaugc cauugacaau     2220

gcuuacacuu ccccuucuaa caagaaagcc cuucggcaag uuuugagagu ggucgaugac     2280

aucaagaggg cagcugacgg acaggauccc agcuggcugu auguggaaac agccgacggc     2340

gggggaacgc cagguaagcg aacccgcgca cggcagcacc aguuacaaga gauuuacgcc     2400

aaugcugcgc augagcucau cgauacugcc gucagaggcg aacuggagga caaaauauca     2460

gauaaggcag acuucaacga uaggcuugug cuguauuuua ugcaaggggg acgugacauu     2520

uacacaggcg cucccuugaa uaucgaucag cucaguuccu augacauuga ucacauccug     2580

ccucagagcc uaauaaaaga caacucucug gacaaucgcg uucucgugca ugccaucauu     2640

aaccgggaaa agaaugccac cuucgcuuca acaaucuacg cacagaaaau gaacgccacu     2700

uggagacagu ggcacgaggc gggucugauu agugggagga agcuccgaaa uuugcagaug     2760

cggccagauc aaaucgacaa guaugcuagc ggcuuuguag cccgccagcu ggucgaaacc     2820

agacagauaa uuaaauuaac ggagcagauc guggcagccc aauacccgga uaccaagauc     2880

auugcuguua aagcaggacu cucccaccag cugagggagg aacuugaguu ccccaagaac     2940

cgggacguga accauuacca ccaugccuuu gaugcguucc uggcugcccg aaucggcaca     3000

uaucuacuca aaagauuucc uaaucugcag gcauucuuua cuuacgggaa guucaccaag     3060

gccgacguca aaaaguugcg cggauuuaac uucauuaggg auauaacaca cgcugaagac     3120

aaaaucgugg ccaaggauac uggugaggua gugugggaca aacagcguga uguugacgag     3180

cuggaucgga uuuauaauuu caagagaaug uuaaucaccc acgaagucag guuugagacg     3240

gcagaccucu ucaaacaaac aguguacggc gcucgcgacu cuaaggaagc cgggggaucg     3300

aagcagcuga uuccaaagaa gaaagauauc cagcuuauau uuaugggcgc gaucagcgag     3360

cgaaccagac ccauucugcc uuuguga                                         3387


<210>  2808
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2808
auggacgaga agauggauua cagaaucggc cuggacauug ggauagccag cgugggaugg       60

gcuguccucc agaacaauuc cgaugacgaa cccguuagga ucguggaucu ugguguacgg      120

auuuucgaca ccgcagagau cccuaaaaca ggcgaaucuc uggccgggcc acgccgagcg      180

gcucguacua cgagaaggcg guugcgcaga aggaagcacc gguuagaucg cauuaaaugg      240

cuguuugaga accaaggacu aaucaauaua gacgauuucc ucaagcgaua uaacauggcc      300

ggccugccgg acguguacca gcuuagauau gaagcacugg auaggaaauu gaccgacgag      360

gagcucgccc agguccugcu gcauaucgcu aagcaccggg guuuucguuc aacacgcaag      420

gcagaaacug ccgcuaaaga gaauggggcc gugcucaagg cgaccgauga aaaccagaaa      480

agaaugcaag agaagggaua caggacaguu ggcgagauga uuuauuuaga cgaagcauuc      540

cggaucggcu gcaguuggag cgagaaaggg uacauucuga cuccccgaaa uaaggccgaa      600

aacuaucagc auaccaugcu ucgcgcuaug uugguggagg aagucaagga gaucuuuucc      660

ucucagagaa ggcugggaaa uaaaaaggcc acagaggaac uagaggaaaa auaccucgag      720

auaaugaccu cgcagcgguc auucgaccug gguccuggca ugcaaccaga ugggaagccc      780

agcccuuacg caauggaagg auuuagugac cgcgugggca aauguacguu ccuuggugau      840

cagggggagc ugagaggagc uaagggcacu uauaccgccg aguacuuugu agcguugcag      900

aaaauuaacc acacaaagcu cguuaaucag gacggcgaaa cuaggaacuu caccgaggag      960

gaacgucggg cccugacauu acugcucuuu acccaaaagg aagugaaaua ugcagcuguc     1020

cgaaagaaac uugggcugcc agaggauauc uuguucuaca aucuaaacua uaagaaagcc     1080

gcuacgaagg aggaacagca gaaggagaau caaaacacug aaaaagccaa guuuauugga     1140

augcccuacu aucaugacua caaaaagugc cuggaggaga gagugaaaua ccucacagaa     1200

aacgagguga gggaucuguu cgacgaaauc gguaugaucc uuaccuguua uaagaaugau     1260

gacucccgca cugagcggcu ggcaaaguug ggccucgucc cuauugaaau ggaggggcug     1320

uuagccuaca caccaaccaa auuccagcac cugucuauga aggcuaugag aaacauaauc     1380

ccguuucuag agaaaggaau gacguaugau aaggcgugcg aagaggcagg cuacgacuuc     1440

aaagccgaca gcaaggguac caagcagaaa cuccugacag gggaaaaugu uaaccagacu     1500

auuaaugaga ucaccaaccc cgugguaaag agguccgugu cacaaacagu caaagugaua     1560

aaugcuauua uccgcacuua uggcaguccu caggccauca acauugaacu ugcacgagag     1620

augagcaaga ccuuugagga acggcguaaa aucaagggag auauggagaa aagacagaag     1680

aauaacgaag acguuaagaa acagauucaa gaguugggca agcugucucc aacagggcag     1740

gauauacuca aauacaggcu guggcaggaa cagcaaggaa ucuguaugua uuccgguaag     1800

accauucccc uugaggagcu guucaaaccu ggcuacgaca ucgaucacau cuugccauac     1860

ucaauuacuu uugacgauag cuuccgcaau aaggugcucg ucacgaguca ggaaaaccgg     1920

cagaagggga auagaacacc cuaugaguac augggaaacg acgaacagag guggaaugag     1980

uuugagaccc gagugaaaac uaccauacgc gauuauaaga aacaacagaa gcugcugaaa     2040

aagcauuucu cugaagagga acgguccgag uuuaaggaaa gaaacuuaac agacaccaaa     2100

uacaucacua caguaauuua uaacaugauc aggcagaauc uagagauggc cccgcucaac     2160

cguccugaga agaaaaagca aguucgcgcu gugaauggcg ccauuacggc auaccugcgg     2220

aaaagauggg gucuucccca gaagaacagg gaaaccgaua cucaccaugc gauggacgcu     2280

gucgugaucg ccugcuguac agaugggaug auacagaaga ucagccgaua uaccaaaguc     2340

cgggagcgcu gcuacucgaa gggcacagaa uuuguggacg cagagacugg agaaauuuuu     2400

agaccagagg acuacucuag ggccgagugg gaugaaaucu ucggcguuca cauuccuaaa     2460

cccugggaga ccuuucgggc ugaauuggac gugcgaaugg gggaugaccc aaagggauuc     2520

cuggauacgc acuccgacgu agcccucgag cuggauuauc ccgaauacau cuaugagaau     2580

uuaagaccua uauucgucuc acgcaugcca aaccauaaag ugaccggugc agcucacgcc     2640

gacacaauua ggaguccgcg ucauuuuaag gaugagggca ucguucugac uaaaaccgcg     2700

cuuacagacu ugaagcucga uaaggacggg gaaauugaug gauacuauaa uccccagagc     2760

gaccugcuac uguacgaggc ccucaaaaag caacugcuuu uguauggcaa cgacgcuaaa     2820

aaggcauucg cccaggauuu ucacaaaccu aaggcugacg guacugaagg gccagugguc     2880

cggaagguga aaauccagaa gaaacagacc augggcguau ucguggauuc cggaaauggc     2940

auagccgaga acgggggaau gguuagaauc gacgucuuua gggugaaugg caaguacuac     3000

uucgugcccg ucuauacggc agauguugug aaaaaggugc ugccuaaccg cgcgucuaca     3060

gccaauaagc cauacagcga guggcgaacc auggaagaca aagauuucuu auucucacuc     3120

uauaguagag accugguaca uauuaagucc aaaaagggua uccccauuaa aaacgucgau     3180

gggagccuug gagagucuaa ggaaguguac gcuuauuaca ucggcgccga cauaucaggu     3240

gcaaagauug agggcaaagc ucacgauagu cgguauacuu uuagggggau cggaguucaa     3300

aagcuggaau ugaucgagaa augucaggug gacauucucg gccacaucag cacagucaag     3360

aaugagaaac gcaugggguu cuccuga                                         3387


<210>  2809
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2809
augaacgaca agaaucagaa auacagacug ggccucgauc uugggaccaa cagcaucgga       60

uggugcauuu auucccugga cgugaauaac aagcccgaga cauugauaga uauggguguc      120

aggaucuucu cugacggccg ggauccuaaa acuaaggaac cauuagccgu ugagcgccga      180

acggcucgug ggcugagaag gauucuacac cggcgcaaac ucagaaggcg gcaaauguuu      240

cgccugcuuc aggaacaggg acugaugccg aaugacgcau cagaggccgc ggcuuugaag      300

agucucaacc ccuacgaacu gcgagugaag gcccuggaua ccaaacucga gccuuaugag      360

uuaggcagag cacuguucaa ucuuagcgua aggcgggguu uuaaguccaa ccguaaagaa      420

gccgugcagg agaaggaaaa agacucucaa aaugaugcua agucgcagaa cgagaagugu      480

gagcauuugg caaaagccau caaggacuca gggugccgca cacugggaga guuccuaugg      540

aaacacaaga auacugagac ccagcagaca gaaaacgaug cuagaaauau uggcaugagg      600

uuugucccag gccggagcaa cuacuauccc acccgacgca uguacgugga ggaguucgac      660

gccaucagaa aagcgcaaga accuuauuac agugggguua auugggaugc acuccaugag      720

aagcuguuuu accagaggcc acuucgggcc caggaacgcg gaaaauguag auauaugccc      780

gacaaggaga ggacuuucaa ggcuaugccu ugcucccaga aaauacguau ccugcaagaa      840

guguacaaca uggacuauau ugaugaguac gguaagacaa ucccauugga agaggcccag      900

accgacucuc ucauuagccu guuagauaaa cgggagaagc uguccuuuaa agcaaugaag      960

aaggaacucg gcaucgacga gucauguacg uucaaccuug aacgaggggg aagagaguau     1020

cugcagggca augcugccgc ggucaaauug aggaacaaga aacgcuuugg ugaacuaugg     1080

gauagucuga gccucgccaa gcaagacgca auaguggaga aacugauuac ugcugaugag     1140

gacgccgaaa ucauuucuau gcuugcucag uacaaucuga ccgaugagca gaagaagaac     1200

aucgccguaa caguuuugcc guccgggacu gcaaugcucu gcaaagaagu gaccgagaug     1260

cugguccaga agauggagca aaauaaaauc cccuuaacag uggccgugca gaugcuggga     1320

uauaccuacg cugaccaguc aguccgggaa gaggauacgc uaccuuacua uggcgcgauu     1380

cucacuggca gcacaauggg ggcacaccca gaagccgacg agagugaacc cgagcugaag     1440

uacggaaaaa uaucuaaccc uaccguucau guggcucuua aucagacuag aacaguagug     1500

aacgccuuga ucaaggcaua ugguaagcca caacagauug ucguggagcu guccagggac     1560

cucaaagcca gccgcgaagc uaaggcccga auccagaaaa ccauaacgau gaaucagaag     1620

cggaacgagc gugauaauaa aaacauuacc gacaucacaa agauccccua cccgaauaga     1680

aacgauaggc ugaaauaucg ccuuugggaa gaacugggcu cggggucuaa uuccuucuca     1740

cggaaguguu uguacugcgg aaagccuauu aguggcagcg aaaucuuuac ugagaacauu     1800

gagauagaac acauccuccc cuucgguaga acccuguucg acggggagac aaacaaaacu     1860

guugcacaca ccgauuguaa uagggacaag ggcgaucgau ccccauuuga agcguucgga     1920

ucuagcccua acggcuauaa uuggcaugag auuugcgcuc gcgccaacuc acugaaaaau     1980

cccgcaaagc ggacauuauu ugccgaaaac gcuauggaga aauucgagaa ggacaauagu     2040

uuuauccaaa gacagcuaac cgauaacgcc uaccucucca ggagcgcacu gcguuaccuu     2100

cgcuguaucu gcaaggacgu guggucuauu aaugggggaa ugacuaaauu gcugcgggau     2160

aaguggaaca uagacucaau ccucaaaaga aagauugaug acccagaaau cgcucacuuc     2220

gagcugaaaa augaccagau uggucaguau aagaagaaca gguacgauca ucgacaccac     2280

gccuuagacg cgagugucau cgcccugaua gaucgggcua uggugcaaga aaucagccag     2340

cuuaauaaaa uucgcuugaa gaacagaauc gaggcacccc agaugccugu acucaggucc     2400

gagcugauug aaaaaguuaa gcauaucacg gugucuuuua aaccagacca cggccggcaa     2460

gggaagcuau cgaaggagac acugcucgga aaaauaaaga aaauugaaaa ggucgauauc     2520

gacagcauuc agaccgauga caucgccucc auaaaagaug acgcuauccg aacuagauuc     2580

gagaccaagc gcagggaacu gggcgauauu acaaaagcca aggcagcgcu uaagaccgag     2640

uuuccgaaau uggacguguu caagacuuau uacgucucac guacacccau cgugagucug     2700

acggagaaaa auauuaacuc uaucguugau gaaaagauaa aagagaaguu aaagaccuuu     2760

auuaaagaac augccgaccu cagcuucgcu gagcagcugc agaaguuuuc cgaaacugag     2820

uggaucggua aaccuucagc cgcagggaag ccaggcaaaa aguaucggau cgagaaggug     2880

agaugcauua acuggggaca aacacccauc accauuacaa gcucugcugu accuagguac     2940

cuuuguccag aagacuaucu ggccgcaguc guguggcaga uacccuccaa aaagaaagau     3000

acugagccgg aauacaaggc caccuucuug cgccgagacg aguucgaugu uaauaacaaa     3060

ccuaagaagc ucgaaaaacc acacgacgcu gcgcaguaca ucugcaugcu guauaaggau     3120

gacuaccuag aguuuacgga gaguggcgcc acccaucugu guagaauugc agggcucccc     3180

gcuagccaga auaacaaaau cgauauucgg ccuaucuaug ccgugucuga cugcaaggau     3240

uggauaaucg ccacaaauga aaacaugcug gagcccuguu ggaaaccaca aaaggaagag     3300

aagaggauuu ccgucaaugu gcuuuucgga aaacacaagg cucgcucagu aacugugaac     3360

ccuaucgguc guguuuuuag aaaauga                                         3387


<210>  2810
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2810
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaaug uagagaggcg gacaaaccgc caaggacgac guuugacuag aaggaagaaa      180

caucggcgcg ugagauuaaa ucaccuguuu gaagagucag gccuaauuac ggacuucacc      240

aaggucagua ucaaccucaa uccuuaccag cugaggguga aaggucuuau agaugaacug      300

agcaacgagg aguuguuuau cgcccucaag aauaugguua agcaucgggg gauuuccuau      360

cuggacgaug cgucugacga uggaaacucg ucagugggcg acuacgcuca gaucgucaaa      420

gaaaauagca agcagcugga gacaaaaacu ccaggccaaa uucagcucga acgcuaucag      480

aaguacgggc aguuacgagg agauuucacc guggaggagg acgguaaaaa gcacagacug      540

aucaacguau uuccgacaag ugccuauagg uccgaagcac uucggauauu gcaaacccag      600

aaggaguuca auccccagau uacugaugaa uuuaucaacc guuaccugga gauucuaaca      660

ggcaaacgca aguauuacca ugggccugga aaugaaaaau cuagaaccga cuacggcagg      720

uauacgacua agaaagauag cgagaacgag uacaucaccc ucgacaacau cuucgguauu      780

cugauaggga agugcacauu uuauccagaa gaguaccggg ccgcuaaagc auccuauacu      840

gcccaggagu ucaaucuucu gaacgacuug aauaaccuca ccguucccac agaaaccaag      900

aagcugucag aggaacaaaa gaaucagauc auuaacuacg ugaagaauga gaaagcuaug      960

ggaccugcca aguuauuuaa auauaucgcg aagcugcuca guugugaugu cgcagacaua     1020

aagggcuacc gaauugauaa aagcgacaag gccgaaaucc acacguucga ggcuuaccgc     1080

aaaaugaaga cucuugaaac aaucgauuuu gagaaaaugu cuagagacca gcuggauaag     1140

uuggccuaug ugcuaacccu gaacacugaa agggagggca uucaagaggc acucgaccac     1200

gaguucgcug augggaauuu cucccaggaa cagaucgacg aacuggugca guuucggaag     1260

gccaacucaa gcauuuucgg aaaagguugg cauaauuuua gugucaaacu uaugauggag     1320

cugauaccag aguuguacgc gacaucugag gaacaaauga ccauccucac gcgccugggc     1380

aaacagaaga ccacauccag cucgaacaaa acuaaguaua uugaugaaaa guuacugacc     1440

gaggaaaucu acaaucccgu uguggccaaa ucuguaagac aggcaaucaa gauugugaac     1500

gcugccauaa aagaguaugg ggacuucgac aauaucguca uugagauggc uagggaaaca     1560

aacgaggaug acgaaaagaa agccauccag aagauucaaa aagcaaacaa ggaugagaag     1620

gacgccgcua ugcuaaaagc ggcaaaucag uacaacggac gugccgaacu cccucacucc     1680

guguuucaug gccacaagca gcuggcuacu aaaauccggc uuuggcacca gcaaggugag     1740

cgaugcuugu auaccgggaa gacaauauca auccaugauc ugauuaauaa cccaaaucag     1800

uucgagaucg accacauucu cccgcugagu aucaccuuug augacagccu ugccaacaaa     1860

guucuggugu acgcaacugc caaucaggaa aagggccaga gaacgcccua ccaggcuuug     1920

gauuccaugg acgaugccug gucuuucagg gagcucaagg cauuuguccg cgaaagcaaa     1980

ucacugagua acaagaaaaa ggaguauuua cugacagaag aggacauauc ccgguucgau     2040

gugagaaaga aguuuauuga gaggaaucua guagacaccc gcuacgcgag ccgaguugug     2100

cucaacgcuc ugcaggaaca uuuccgggcc cacaagacug acaccaaagu cucugugguc     2160

cguggacagu uuacaucaca acuuagaagg cauuggggca ucgagaagac ccgcgauacu     2220

uaucaccacc augcagugga cgccuugauu aucgcugcca guagccagcu gaaucucugg     2280

aaaaagcaga aaaacacacu gguuaauuac uccgaaaacc aguuacugga uauagagacg     2340

ggggagcuua ucucugacga ugaauauaag gagucggugu ucaaggcacc uuaccaacac     2400

uucguagaca ccuugaaaag caaggaauuu gaggauucca uucucuucuc auaucagguc     2460

gacaguaaau uuaaucggaa gaucucugau gcuacuauuu acgccacaag acaggcgaaa     2520

gugggaaagg acaaaaagga ugaaaccuac guucugggua agaucaaaga cauauauagc     2580

cagacaggcu acgaugccuu cauuaagauc uaucaaaaag acaaguccaa auuucuaaug     2640

uacaggcaug acccacagac uuucgagaag gugaucgagc ccauucugga aaacuauccu     2700

aacaaggaga ugaaugaaaa agggaaggag gucccaugua accccuuucu caaauacaag     2760

gaggaacacg gagauuauau ccgaaaauac ucaaagaagg gcaauggucc ggagauuaaa     2820

agccugaagu acuaugacuc uaaacuuggg aaccacauag auaucacccc uaaggacucc     2880

aauaacaaag ugguauugca gagugugagc cccuggcgcg cugauguuua cuucaauaag     2940

acgaccggca aguaugaaau ucugggauua aaauacgcag accuccaguu ugagaagggc     3000

acagggacuu auaaaaucuc ucaagaaaag uacaacguca uuaaaaagaa ggagggagug     3060

gauuccgacu cagaguucaa auuuacccug uauaagaaug aucuucuguu gaucaaagac     3120

acagaaacua aggagcagca acucuuccgg uuucugagua gaaccaaacc aaacgugaag     3180

cauuacgucg aacuaaaacc uuacgauaag caaaaguucg aggguaauga aagccugaua     3240

aacguucucg gcgccguggc uaaaggggga cagugccaga agggcaucaa uaaacccaac     3300

auuucgaucu auaaggugag gacggacgua cuggguaauc agcacauuau caaaaacgag     3360

ggggacaagc caaaacuuga uuucuga                                         3387


<210>  2811
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2811
auggacgaga agauggauua cagaaucggc cuggacauug ggauagccag cgugggaugg       60

gcuguccucc agaacaauuc cgaugacgaa cccguuagga ucguggaucu ugguguacgg      120

auuuucgaca ccgcagagau cccuaaaaca ggcgaaucuc uggccgggcc acgccgagcg      180

gcucguacua cgagaaggcg guugcgcaga aggaagcacc gguuagaucg cauuaaaugg      240

cuguuugaga accaaggacu aaucaauaua gacgauuucc ucaagcgaua uaacauggcc      300

ggccugccgg acguguacca gcuuagauau gaagcacugg auaggaaauu gaccgacgag      360

gagcucgccc agguccugcu gcauaucgcu aagcaccggg guuuucguuc aacacgcaag      420

gcagaaacug ccgcuaaaga gaauggggcc gugcucaagg cgaccgauga aaaccagaaa      480

agaaugcaag agaagggaua caggacaguu ggcgagauga uuuauuuaga cgaagcauuc      540

cggaccggcu gcaguuggag cgagaaaggg uacauccuga cuccccgaaa uaaggccgaa      600

aacuaucagc auacaaugcu ucgcgcuaug uugguggagg aagucaagga gaucuuuucc      660

ucucagagaa ggcugggaaa ugagaaagcc accgaagaac uagaagagaa guaccucgaa      720

auuaugacgu cgcagcgguc auucgaccug gguccuggca ugcaaccaga ugggaaaccc      780

agcccuuacg caauggaggg auuuagugac cgcgugggca aguguacuuu ccuuggugau      840

cagggggagc ugagaggagc uaaaggcacc uauacagccg aauacuuugu agcguugcag      900

aagauaaacc acacuaaacu cguuaaucag gacggcgaga ccaggaacuu cacagaagag      960

gaacgucggg cccugaccuu acugcucuuu acgcaaaagg aggugaagua ugcagcuguc     1020

cgaaagaaac uugggcugcc agaggauauu uuguucuaca aucuaaacua uaaaaaggcc     1080

gcuacuaaag aagaacagca gaaggaaaau caaaacacag agaaggccaa auuuaucgga     1140

augcccuacu aucaugacua caagaaaugc cuggaggaaa gagugaagua ccucaccgag     1200

aacgaaguga gggaucuguu cgacgagauu gguacuaucc uuacauguua uaaaaaugau     1260

gacucccgca ccgaacggcu ggcaaaguug ggccucgucc cuaucgagau ggaggggcug     1320

uuagccuaca cgccaaccaa guuccagcac cugucuauga aagcuaugag aaacauuaua     1380

ccguuucuag aaaagggaau gacauaugau aaagcgugcg aggaagcagg cuacgacuuc     1440

aaggccgaca gcaaagguau caagcagaag cuccugacug gggagaaagu uaaucagacc     1500

auuaacgaaa ucacaaaucc cgugguaaag agguccgugu cacaaacugu caaagugaua     1560

aacgcuauua uccgcaccua uggcaguccu caggccauca auauugagcu ugcacgagag     1620

augagcaaga cauuugagga gcggcguaaa aucaagggag auauggagaa aagacagaag     1680

aacaaugagg acguuaagaa acagauucaa gaauugggca agcugucucc aaccgggcag     1740

gauauacuca aauacaggcu guggcaggag cagcaaggaa ucuguaugua uuccgguaag     1800

acuauucccc uugaggaacu guucaaaccu ggcuacgaca ucgaucacau cuugccauac     1860

ucaauuacgu uugacgauag cuuccgcaac aaggugcucg ucacaaguca ggagaaucgg     1920

cagaagggga acagaacccc cuaugaauac augggaaaug acgagcagag guggaacgag     1980

uuugaaacuc gagugaaaac cacaauacgc gauuauaaga aacaacagaa gcugcugaaa     2040

aagcauuucu cugaggagga acgguccgaa uuuaaggaga gaaauuuaac cgacacuaaa     2100

uacaucacaa cgguaauuua uaacaugauc aggcagaacc uagagauggc cccgcucaau     2160

cguccugaaa agaaaaagca aguucgcgcu gugaacggcg ccauuaccgc auaccugcgg     2220

aaaagauggg gucuucccca gaagaauagg gagacugaua cacaccaugc gauggacgcu     2280

gucgugaucg ccugcuguac cgaugggaug auacagaaga ucagccgaua uacaaaaguc     2340

cgggaacgcu gcuacucgaa gggcacugag uucguggacg cagaaaccgg agagauuuuu     2400

agaccagagg acuacucuag ggccgaaugg gaugagaucu ucggcguuca cauuccuaaa     2460

cccugggaaa cguuucgggc ugaguuggac gugcgaaugg gggaugaccc aaagggauuc     2520

cuggauaccc acuccgacgu agcccucgaa cuggauuauc ccgaguacau cuaugagaac     2580

uuaagaccua uauucgucuc acgcaugcca aaucauaaag ugacaggugc agcucacgcc     2640

gacacuauua ggaguccgcg ucauuuuaag gaugaaggca ucguucugac caaaacagcg     2700

cuuacugacu ugaagcucga uaaggacggg gagauugaug gauacuauaa cccccagagc     2760

gaccugcuac uguacgaagc ccucaaaaag caacugcuuu uguauggcaa ugacgcuaaa     2820

aaggcauucg cccaggauuu ucacaaaccu aaggcugacg guaccgaggg gccagugguc     2880

cggaagguga aaauccagaa gaaacagacg augggcguau ucguggauuc cggaaacggc     2940

auagccgaga augggggaau gguuagaauc gacgucuuuc augugaacgg caaguacuac     3000

uucgugcccg ucuauacagc agauguugug aaaaaggugc ugccuaauag ggcggccacc     3060

gcucacaagc cauacgguga auggaaagua auggaggaca aggacuuuuu auucucucuc     3120

uauagccgcg aucugauuca caucaaauca aagaaagaua uucccaucaa gauggucaac     3180

gggggaaugg aaggcauaaa ggagacuuac gccuauuaca uuggugcaga caucagugcu     3240

gccaauaucc aaggcauugc acaugauucc cgauauaaau uuagagggcu uggaauccag     3300

agccuggacg uguuggaaaa gugucagauu gacguucucg gccacguguc ugucgugcgg     3360

ucagagaaaa ggaugggguu caguuga                                         3387


<210>  2812
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2812
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cguacacaaa aaugccagaa uuuuccccgc ugcacaggcc      120

gaaaacaaug uggagaggcg gacaaaccgc caaggacgac guuugacuag aaggaagaaa      180

caucggcgcg ucagauuaaa ucacaucuuu gaagagucug gccugauuac ggacuucacc      240

aaggugucaa ucaaccuaaa uccuuaccag cucaggguua aaggucugac agaugaacuu      300

aguaacgagg agcuguuuau agcguugaag aauaugguga agcaucgggg gaucagcuau      360

cucgacgaug cuuccgacga uggaaacucu ucggucggcg acuacgccca gauugugaaa      420

gaaaauucaa agcagcugga gacuaaaacc ccaggccaaa uccagcugga acgcuaucag      480

aaguacgggc agcuccgagg agauuucaca auugaggagg acgguaaaaa gcacagauua      540

aucaacguau uuccgaccag cgcauauagg gccgaagcuc ugcggauacu ucaaacucag      600

caggaguuca auccccagau uacagaugaa uuuaucaaca guuacuugca aauucugacc      660

ggcaagcgua aauauuacca ugggccugga aaugagaagu cccgcacgga cuacggcaga      720

uauacuacca aaaaggauuc ugaagacgag uacaucacac uagacaacau cuucgguauu      780

cucauaggga aaugcacuuu uuauccagag gaauacaggg cagccaaggc uagcuauacc      840

gcccaggagu ucaaccugcu uaaugaucug aacaauuuga caguucccac cgaaacgaaa      900

aagcucuccg aggaacagaa gaaccaaauc auuacuuacg ugaaaaauga gaaggcgaug      960

ggaccugcaa aacuguuuaa guauaucgcc aaauuacugg cuugugacgu cgccgauaua     1020

aagggcuacc ggauugacaa gucagauaaa ccagagaucc acacauucga agcauaccga     1080

aagaugaaaa cccucgagac uaucgacgug gaacagaugg agcgcgaaaa acuugauaaa     1140

cuggcuuaug uguugacacu aaacaccgag agagagggca uucaggaagc caucgagcac     1200

gaauuuagug acgggacguu cagccaggag caagucgaug agcugguuca guucaggaag     1260

ucuaauuccu caauuuuugg aaaggguugg cauaacuuca gcgugaaacu caugauggaa     1320

cugauacccg agcuuuacgc gaccagugaa gaacagauga caauccugac ucgguugggc     1380

aagcagaaac gcaccucuuc cagcaauaag acaaaauaua uugacgaaaa gagacucacu     1440

gaggagaucu acaacccugu aguggccaag ucggucaggc aagcaaucaa aauugugaau     1500

gcugccauaa aggaauaugg ggauuuugac aacaucguua uugagauggc ucgugaaaug     1560

aaugaggacg augaaaagaa ggccaucaaa aagauucagg aggcaaacaa aucugagaag     1620

gacgccgcua ugcggaaggc ggcaaaucag uacaacggaa aagccgaacu gccacacucc     1680

guguuccaug gccacaagca guuagcuacc aaaauccgac uguggcacca acagggugag     1740

agaugccuau auacagggaa gaccauauca auccaugauc ucauuaacaa uccgaaccag     1800

uuugaaaucg accacauucu gccccuuagu aucacuuucg augacagcuu ggccaauaaa     1860

guccuggugu acgcaacggc caaccaggag aagggccaaa ggacaccuua ccaggcucuc     1920

gauuccaugg acgaugccug gucuuuucgc gaacugaagg cauucguacg ggagagcaaa     1980

acccuuucaa auaagaaaaa ggaguaucug uugacugaag aggacauaag uaaauuugau     2040

guuagaaaga aguucauuga aaggaaccuc guggacaccc gcuacgcguc ccgagucgug     2100

cugaaugcuu uacaggagca uuuucgggcc cguaaaaucg auacaaaggu cagcgugguu     2160

agaggacaau ucaccucuca gcugaggcgc cacuggggca uugacaaaac ucgggacaca     2220

uaucaucacc acgcagugga ugcccuaauc auagcugccu caagucagcu caaccugugg     2280

aagaaacaga agaauacgcu uguaagcuac uccgaggacc aauugcugga uaucgaaacc     2340

ggggagcuca uuucugacga ugaauauaag gagucggucu uuaaagcacc auaccagcau     2400

uucguggaca cuuuaaagag caaagaguuc gaggauucca uccuguuuuc auaucagguu     2460

gacaguaagu ucaacagaaa aauuucugau gcuacaaucu acgccaccag gcaggcgaag     2520

gugggaaaag acaagaagga ugagacauac guccugggua aaauaaagga cauuuauagc     2580

caagccggcu acgaugcuuu uaucaaaauc uaucagaagg acaaauccaa guuccuuaug     2640

uaccgacacg acccccagac uuuugaaaag gugauugagc cuaucuugga aaauuaucca     2700

aacaaagaga ugaaugaaaa ggggaaagag guacccugua acccguuccu caaguacaaa     2760

gaggaacaug gagauuauau ucgcaaguac ucaaagaaag gcaauggucc ugagauaaag     2820

agccugaaau acuaugacuc uaagcuaggg aaccacaucg auauuacccc caaaaacucc     2880

aauaacaagg ugguucugca gagugucagc ccauggcggg cagacgugua cuuuaauaag     2940

acgaccggca aauaugaaau ccucggacug aaguacgccg aucuucaauu cgagaaaggc     3000

acagggacuu auaagauuuc ucaggaaaaa uacaacgaca ucaagaagaa agagggagug     3060

gauuccgacu cagaguuuaa guucaccuug uauaaaaaug aucuguuacu cauaaaggac     3120

acagaaacua aagagcagca acuguuuaga uuccuuagua ggagcaugcc uaacaagaaa     3180

cacuacgucg aacugaagcc cuacucgaag gauaaauuug agaagaauga gaccuugauc     3240

gaaauucucg guccaacgga caaauccggc cguugcauca aggggcuggc uaaaucuaac     3300

cuaagcauuu ucaagguucg cacagacauc cugggaaauc agcauauaau uaagaacgag     3360

ggcgauaaac ccaagcucga cuucuga                                         3387


<210>  2813
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2813
augaccaaga uccugggccu cgaccuuggg acaaacagca uuggaugggc cguggucgau       60

gagaaaaagg agaaaauacu ggguaugggc uccagaaucu uccccgaagg gguugugaau      120

auuggagaag gcgaggguag ggaaacuucu aagaacgcuu cacggacgga cgcacgcggg      180

acccgacguc aguuuuucag aaggcgguug cgcaaaagau acuuacugag ggagcuagcc      240

aagaagggac ucugcccuau caauuauaca cugguaaaag aguggaacga agcgcaaauu      300

uuuaagaaua aacagcuuca ggagugguuc aagcugaacc cauacgaauu gcggaguaaa      360

gcuauccagg agaagauaag ccucgaggaa cugggccgca ucuuuuauca cuucauucaa      420

cgaagaggcu uucaguccaa uucuaggucg gccggggugg auaacaucga gaagucaguc      480

auuuucaaag gagacacuaa gaccgguaaa aucggcauaa gcgaaacacu ggagaguauu      540

aagaauacca aaacucucgg guccuacuua cauacaaucu auccgaagca gaacaaaccc      600

uacucuggac acaaggaacg gauucguaau cgcuauacca cgagacagau guacaucuac      660

gaguuugaga cuaucuggga auaucaaaag caguucuaca gcgagcuguc caacgaacuu      720

aaaaccgugu ugggcgguag gaagaaagau ggguauaagg aggacgggau ucuguuucau      780

cagcggccuc uacgaucaca gaaacaccuc guuggcuacu guaguuucga accaaagaag      840

acaaaaugcc aaauaaauac uauccccuuu gagcuguucc gcauuuauca gugggugaac      900

accgucgauu guaaugagaa ggugacaagc gaagacagag gcaaaauggu agagcuucug      960

uugucuaagg aaaaaguuuc cuucaaggag aucaggaagg ugauaaacaa aucagauggg     1020

uacuaucagu uuaacuacaa gaaugacgau aaaauugucg gaagcuacac caucaguaac     1080

cucucuaaua agaaauucuu ugguaagaag ugguucgacu uuuccgauaa agaacaagag     1140

gacaucuggc augugcugag cuucuuugac gaucgggaca aguuaaaaca guaugcaauu     1200

aaguacuggg gcuucaacga ggaaaaagcc gugaagaucu cgaaauuuaa ucugaaggag     1260

ggguauucua accucucccg caaggcuauu cacaauauac uuccuuuccu gaaauuggga     1320

uuuauguacg augucgcagu uggccuaggu ggggugaaga acgcccuggc ugacaaaugg     1380

aucgaaaaug agcaguucau ucucgauaac guaccaucag uggucagaag uaauuuuaag     1440

ggcggauaua ucgagccccu gaaacagaug cuuaaguacg aguucgacgu gagcgauaag     1500

caacuguuga aacucuauca ccauuccucu gccaucgaaa cgacugaacu guuaaagaaa     1560

cugccuauua acagcgaggc ggacaaggaa auacagucaa ucaaaaaucc aguugugauu     1620

acagcacuau ucgagaucag gaagcucguc aacgagauua ucgauaccua cggcaaguuu     1680

gacgaaauaa aaguggagcu ggcccgugau cuuaaggcua guaaauccaa gcgguacgac     1740

acucgaagag aacaguugag gcuggagaaa gaaaaugacc gcaucaagau ugagcucgag     1800

aaccuggggc agcggaucaa ccacauuaca auccuuaagu auaaacugug ggaagagugc     1860

gauaagagau guccguucac cgguaaaaau auagaaguaa acgaguuguu uagcggagaa     1920

guucaaauug agcauaucuu ccccuggucu aggucacuca augacaguuu uaugaacaag     1980

acgcugugcu ucgccgauga gaaucgcgca aaaggcaaca agaccccuua cgaauuuuau     2040

agcgagcagg gggaacagaa augggaggag auuaagaucc aggcuuuauc cuguuucaag     2100

aauaaaccaa acuaccccaa ugccuaucaa aaguuuaaac aguucgugaa gaaaaagcac     2160

gacgaagauu uuauaucucg acagcugaac gacacacggu acaucucgaa ggaggcgaaa     2220

aauuaucuaa gcaagauuug caacaauguc cagguggccc cuggacaaau gacugcaacc     2280

cuccgucaca aauggggccu gaacacaauc cuuaauaagg augacgauac uaaaaccaga     2340

gacgaucaua ggcaccaugc uauugacgcc uuggucaugg cuuguuccac acgcucacau     2400

cugcaggaac ucaguaagcg gaacagauac cagaaggauu acgaguucaa agacuuucca     2460

augcccuggc aauucuucag gcaggacgcc gaaaagucug uggagcagau ccugguuagc     2520

cacaaaaagu uaaacaccau acugacugug cgaacgcaca aaacaaagaa gaauggugua     2580

gucuauaaaa acauuggggu ggcagcccgc ggccagcuuc acaaggagac cguuuuugga     2640

aaacggacug cuccggaugg caucgaagcg uaccauguga gaaagccuau cgaguccuug     2700

ucaacccaca aacauauuaa uaaggucgug gacgaaacaa ucaggcuccu gauucuaaag     2760

caaguaaaca aacugggagg auucgugaag gguaaaauac ccgagggcac cuuuuauaau     2820

acugaugaaa acgggaauaa gcagccacag aucuuccucc cuaacaaaaa uggaaacccc     2880

guuccaauua agaaaauccg ucugcgcgag aauauuggcg gugcagagca gcuuaagagc     2940

acagucaacc aacacgugaa uccccggucu aaccaccaug uguugaucua caaggacuuu     3000

gaugacaauc ugaaagaaaa ggucguuacg uucuggaccg ccguggagag gaaaaagcag     3060

aaacaggcug uguuucaguu accugaaaac gggaaggaga uaguaacuac acuccaaauc     3120

aacgauaugu uccugcuugg ccugaaggac gaugaaauua auugggagca cccaaaauuu     3180

accuugcuca aggagcaucu guauagaguc cagaaauuca cauccggaga cuacuauuuu     3240

cgaaagcaca ucgaaaguaa ccuaaauggc gagcugggga aagccuacca guacauuaag     3300

ggauucggcg aagguaagac uggguggaaa accuucaacc cgaucaagau aaaagugagc     3360

guuucuggaa agauugagaa aguguga                                         3387


<210>  2814
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2814
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugc uggcauacug       60

aagaaagaua ccggggagau cauucacgca aauuccagau uguuccccgc cgcgacagcu      120

gacaacaaug uuuuaaggcg gucuucacgc caggcccgac guaguacuag aaggaagcgg      180

caucgcgcag ugagacugca cgaucuauuu gaagacuacg gacuccugac ggauuucagc      240

aaaguaucca ucaaccuuaa uccuuauaag cugagggugg agggcaugga cauucaauug      300

accacagaag aacucuuuau cgcccugaaa aacauaauca agcggcgcgg uauuucuuac      360

cuggaugacg cuucggaaga uggggucacu gugucaagcg acuauggaaa ggcaguugag      420

gagaaucgaa aacucuuaag ugaacagacc ccaggccaga uccagcugga gagauucgaa      480

aaguacggcc aagugagggg agauuuuaca gucgaggaga acggggaaaa acggcgucuu      540

auuaaugugu ucuccaccuc uacuuaucgc aaggaggccg aaagaaucuu gaggaaacag      600

caggaguuua acaauaagau aacagacgau uucauugacg acuaccugac cauccuaacg      660

gguaagcgga aauauuacca uggcccgggg aacaagaaaa gccgaacuga uuacggacgc      720

uauaccacaa agaaagacuc cgaaggcaag uacauuacuc ucgauaauau cuuuggugua      780

cugaucggga aaugcaccuu cuaucccgac gaguacagag cuucaaaggc caguuauaca      840

gcgcaggagu uuaaccuucu gaaugauuug aacaaccuca ccguuccuac ggaaacuaag      900

aaacugagcg agaagcaaaa gaagacaauu guggaauacg caaaaaccgc caagacuuua      960

ggagcuucua cacugcucaa guauauagcc aaaauggucg acgcauccgu ggaucagauc     1020

cacggcuaca ggguggacgu caauaagaaa ccagagaugc auaccuucga uguuuaccgg     1080

aagaugcagu cacuugaaac guuuaacgug aaugagaugc cccgcgaggu acuggacgaa     1140

uuggcucaca uucuaacccu gaacacagag agagaaggca ucgaggaagc cauaaauacu     1200

aaacucaagg ggaccuucaa ccaggaucaa gugcuggagc uuguccaguu caggaagaau     1260

aacagcaguc uguuuucuaa aggauggcac aauuucuccu ugaagcucau gauggagcug     1320

auuccugaau uauaugcgac aagcgaggaa cagaugacua uccugacccg ucuagguaaa     1380

caaaagucga aagagaucuc uaagcggaca aaguacauug acgagaaaga aaucaccgag     1440

gaaauuuaua acccaguggu ugccaagucc gugcgacagg caauaaaaau cauuaaugag     1500

gcuacuaagc aguacggcau cuuugaccau aucgucauug aaauggccag acucacgaac     1560

gaggaggaug cuaaaaagga cuauauaaaa aggcagaagg ccaaucugga ugaaaagaac     1620

gcagccaugg agaaagcugc guuccaauac aaugggaaga aagaacuucc cgacucaauc     1680

uuucacggac auaaggaguu ggcaacaaaa auucgccugu ggcaccagca gggcgaaaag     1740

ugucucuaua ccgguaagaa caucccuauu agugaucuga uccagaacca auacaaauac     1800

gagauagacc acauccuucc acugagcuug uccuucgaug acucucucag caauaaggug     1860

cugguauuag ccacugcuaa ccaggagaaa gggcagcgga ccccguuuca ggcccuggau     1920

agaauggacg augcaugguc auauagggag uucaagaguu acguuaaaga cuccaagcua     1980

cucggcaaua agaaaaagga uuaucugcuu acagaggaag acauuagcaa aaucgaggug     2040

aagcaaaaau uuauugaacg caacuugguc gacacccgau acucuucacg ggugguccug     2100

aaugcccucc aggauuucua uaagaaccau cacuuugaca cuacaaucag ugugguucgu     2160

ggacaguuca cgagccagcu gagaaggaag uggggcuuag agaaaucccg cgagaccuac     2220

caucaccaug cuguggaugc ccugauaauu gcagcgucuu cgcaacuucg guuguggaag     2280

aaacagaaua acccccucau cuuuuauaag gaagggcagu ucguagacag cgagacugga     2340

gaaauucugu cccuaaauga ugacgaguac aaagagcugg ucuucaaggc uccuuacgau     2400

cacuuugugg acacacucag aucaaaaacc uucgaagaua guauccuguu uucuuaucaa     2460

guugacagca aguacaacag gaagauaucc gaugccacaa ucuaugcaac ucgaaaagcc     2520

aaacuugaca aagauaaguc aaaagagacc uacguguuga gcaagauuaa ggacaucuau     2580

ucucaggcug guuacgacgc cuucauuaaa aucuauaaua aggauaaauc caaguuucug     2640

auguaccaca aagacaguca gacguucgaa aaggucauag aggaaauuuu acgcaccuac     2700

ccaagcaagg aguuuaacga uaaaaauaag gaggugcccu gcaacccuuu cgaaaaauau     2760

cggcaggaga auggcccaau cagaaaguac ucuaaaaagg ggaacggacc cgaaaucaag     2820

ucccucaaau auuacgacaa gaacauuccg gagucauuua ucaauauuac accuaguaac     2880

agcaaaaaua agguaauacu gaggcaacuu aaccccuggc guacugaugu guauuucaau     2940

caucagaccg aaaaauacga gcuguugggc cucaaguaug cagaccugca guuugagaag     3000

aaaacaggua cuuacucgau caccauugau aaguacaacg aaaucaaaga gaaggaaggg     3060

guuggcgagc aguccgaguu caaauuuacg cuauauaaga augaccugcu ccugauuaaa     3120

gauacaaaga acaaugaaaa gcaaaucuuc cgcuuccuuu cucggaccau gccaaacguc     3180

aaacacuacg uggaguugaa gccuuauaau aaagcuaagu uugacggaaa ccagccccug     3240

auaacuaucu ucggcaaugu ggccaaaggg ggacaguguu uaaagggucu caacaagcca     3300

aauauuagca ucuacaaaau uaagacagau guccugggca acaaauauuu uaucaagaaa     3360

gaaggggaca agcccaaacu uaaguucuga                                      3390


<210>  2815
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2815
auggccgaca gaaucagccu gggccucgau auuggggugg cuuccgucgg auucucuguu       60

cuugacauag auaaggguaa agugaucgag cugggcucaa gguuguuuaa cgcaaccgua      120

gccgaaggga aucaggaccg gcgcaguaug cgaggagcgc guagauuacu gaacaggaau      180

aagcaacggc gccaggauac aggcaaacua uuccagaagu uuggucuccu ggacaacuuc      240

aauaaagaua acuuuuacga cacuuucaau cagaacugga aucccuauga gcuuagagug      300

aaggggcuga cggaacacuu gaccaagaaa gagcucgcug aaagccugua ccaaauuguc      360

aagcauaggg gaaucuccua ugcccugaaa gaugcagacg ccgaugaggg cggcacagac      420

uacucugcuu cgcucaagau uaacucacag gaguuagcag agaaaacucc ugcccagauc      480

cagcugcaac ggcuuaauga uuaugggaag gugcgcggac agguugugau aggugacgac      540

ccagauaacc agaaggucuu gcugaaugug uuuccgacca gcgcuuacga gaaagaagcc      600

aagcagauca uugcgacaca aaaacaguuc uaucccgaga uccuaaccga cgaguuuacu      660

aaggaauacu gcaaaauucu cacacgaaag agagauuacu ucguaggccc ugggaacgag      720

aaaaguagga ccgacuaugg aaucuacaag acggauggcc ggacucugga caaucuuuuu      780

gaagaacuga uaggucacga uaagauuuau ccagacgaau ugcgugcauc cgccgcuucu      840

uacaccgccc agcucuuuaa cguucugaac gauuuaaaua accugcgcau ccucagcuau      900

gaggacggga aacuuacaaa ggaggauaaa gaaaagauua ucgcagagau caaauccaau      960

acuaccacaa uuaacaugcu gaaugugaua aagaaggucg cuggauguuc aaaagaagac     1020

aucaagggcu uuagagugaa cgagaaagac aagcccgaaa uuaguagcau ggccguguac     1080

aggaaagucc auaaggauuu gcuaaagguu ggcguggaca ucucugauug gccuguagag     1140

uucauagaca aacuguccuu cauucucacc cugaauacgg agaacgggga aauccggaag     1200

caacuuaaua accgacuggu gccagaguuu gauuucuuga augacgaucu cauccagcug     1260

auuaucgaca acaaagauuc auuugaaauu aagacuaaua acaaauggca ccgcuucagc     1320

uuaaagacaa ugaauaagcu gaugcccgag augcuagaaa gaccugucga gcagaugacc     1380

cuccugaacg agaugggacu ugugaaaaag gacaaaaaga gguuugaaaa caauaaauau     1440

uugccauaca aggagauagc gaaagauauc uucaacccgg uugccaguaa gucugugcgg     1500

gaagcacuga agauugucaa ugcugugcuc aaaaaguacg gucauaucga cuaucuggua     1560

aucgagaugc cccgcgauaa aaaccuuaag gaggaacagg acaauauuaa agaguuucaa     1620

aacaagaaua agaaagccaa ggacgcugcc uucgaagcau uuguuaaauc cgugggcagc     1680

gagcagagag ucaaggaagc ccugucgaaa aacaggaagu ugcagaugaa gaugcgucuc     1740

ugguaccagc aacaggagau agauccuuau aauggggaga cuaucgacgc uacagaucug     1800

auuaacaauc cagacaaauu cgaaaucgau cacauuaucc cccagucuau auccuuugac     1860

gauucaauca acaauaagac cuuaugcuuc gcgaguauga accaggugaa aggacaaaag     1920

acgccuuacg aguuuaugcu ggaaggccac ggucaggggu augacaaauu caaggcaacc     1980

guccuagaua auaagaacuu cgaccggaac aaacgaaaga auuaccucuu ugaggaaaac     2040

gugagcgaua uugagacaag aaaaagguuc cugucccgca aucuuguuga cacucgguau     2100

ucuagcagag ugguauugaa cucacugcag gauuucuucc augagaagag ugccgacacc     2160

aaagucacag ugaucagggg caaguuuacu uccaauaugc gcaaacacug gcauauugac     2220

aagacccgag aaacauucca ccaccaugcu aucgaugcca gcauaauugc agccacccca     2280

uuucuccgga uguggaagaa aggaggcacu aucuuccccg uuaagguggg ggaggaaucu     2340

auugacaucg agacgggaga aauacuggau gacaaaaagu uugauaaagu cauguacgag     2400

gagccguaca acgguuucgu gucagaaauc augaaugcug acgaucguau uaaguuuagu     2460

caccagguag acaagaaaau gaacagaaag gugagcgaug ccacaaucua uuccaccagg     2520

acuggcaaau uagcaaagga caaaaaggau gcggaguaca uuguugcuaa ggucaaagac     2580

aucuauucug uggacggguu caagaaauuu aagaaagugu acgauaagga caagucgaaa     2640

uuccugcuuu auaaguacga uccucgcacc uucagcgaau uggagcggau aauuuccgac     2700

ugucccgaua aagucgagaa gguucaaaca aauggaaaag ugaaggccgu ggacguauca     2760

ccauuugaaa uguauagaag guaccauggc augaucaaga aauacaguaa gaaagguaac     2820

gggccugcaa ucaagcagcu caaauaucug gauaagaaac uaggcucuca cauugacauc     2880

acccccgcca augcuaacgg aaagcauguc auucugcaga gccucaagcc auggcgaacu     2940

gauguguacc ugaaucacga gacaggcgaa uaugagauaa uggggaucaa auacuccgac     3000

cuuaaguuca acaaaaaugu uggauauggu auuaagaaag auaaguacuu ggaaaucaag     3060

aaaaacgagg aggugucaga aaagagcgag uuuauguucu cucuguaucg caaagaccgg     3120

gucaggauuc aaaacaugaa gacgggcgaa uccguggagu uacucuuuug gaguagaaau     3180

uucagcaaca aaaaguacgc cgaacugaag cccaucuuua aauucguuac cgaggaugac     3240

gacuggccug uguacgggca cgcaaagacu cagauacuua aacgucuggu accaaaggau     3300

ugcaaaaucu ggaaggucaa uacaaccauu uugggagacc cguauuaccu cgagaaggaa     3360

ucugauaacc ccaaagacau ccuggauuga                                      3390


<210>  2816
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2816
augaccaacg gcaagauccu ggggcucgac auuggaauag ccagcguggg ugucggcauc       60

auugaggcua aaacagggaa gguugugcac gcaaauucca gacuuuucuc ugccgcgaac      120

gcugaaaaua acgccgagag gcggggauuu cgcggcucac gacgucugaa uagaaggaaa      180

aagcaucggg uaaaacgcgu gagagauuug uucgaaaagu acgguaucgu cacugacuuu      240

aggaacuuaa aucugaaccc cuaugagcua cgggugaagg ggcucacgga acagcugacc      300

aaugaggagc uuuucgcagc ccugcgcaca auuaguaaac gaagaggaau cagcuacuug      360

gaugacgcug aagaugacuc cacuggcucu accgauuaug caaagucgau agacgagaac      420

aggcggcucc ugaaaacaaa gaccccuggc caaauccagc uggaacgucu cgagaaauac      480

gggcaguuac gcggaaauuu uacuguuuau gaugagaacg gugaagccca cagacugauu      540

aauguguucu caacaagcga cuacgagaag gaagcuagga agauccuuga gacccaggcc      600

gauuauaaca aaaagauuac ggcggaauuu aucgacgauu acgucgagau auugacucaa      660

aaacggaagu acuaucaugg cccagggaau gagaaaaguc gaaccgacua cggacgcuuc      720

agaacagacg gcacuacccu ggaaaacauu uuugguaucc uaauugggaa gugcuccuuc      780

uauccggaug aguacagggc aucuaaagcc agcuauacag cucaggaaua caacuuucuc      840

aaugaccuga acaaucuuaa ggugcccacc gagacgggaa agcuguccac ugaacagaaa      900

gaggccuugg uagaguucgc aaagucaaca gcuacccucg gcccugccaa acuguuaaag      960

gaaaucgcga aaauccugga uuguaagguu gacgagauua agggcuaucg ggaagaugac     1020

aaagggaagc cagaucucca cacuuuugag cccuaccgca aacuuaaguu caaccuggac     1080

agugugaaua uagaugacuu gagcagagaa guccuagaua aacuggccga cauccucaca     1140

cugaacaccg agagggaggg aauugaagac gcaaucaagc guaaucuucc uaaccaguuc     1200

acggaggaac aaauaucuga gauuaucaau gugcggaagu cccagucaac cgcuuuuaac     1260

aaagguuggc auagcuucag ugccaagcug augaaugagu ugaucccaga acucuacgcu     1320

acaucugaug agcagaugac uauucugacc cgauuagaaa aauuuaaggu gaaaaagaag     1380

uccagcaaaa acaucaagac aauugacgag aaagaaauaa cugaugagau cuauaauccc     1440

gucguugcca agucggugag acagaccauu aaaaucauca acgcagccgu aaagaaauac     1500

ggcgacuucg auaagauugu gauagagaug ccuagggaca agaaugcuga ugacgagaaa     1560

aaguuuaucg auaaacgcaa caaggaaaac aaaaaggaaa aggacgaugc gcugaaacgg     1620

gcagccuauc uauacaaugg gacagacaag cucccagaug aggucuucca cgaaaacaaa     1680

caacuggaga ccaagauuag acuuugguau cagcagggag agaggugcuu guacucuggc     1740

aaaccgaucc ccauucaaga acuggugcac aauuccaaca auuuugagau cgaccauaua     1800

cucccucugu cacuuaguuu cgacgauagc cuggcuaaca agguuuuggu guauuccugg     1860

acuaaucagg aaaaggguca gcgcacgcca uaccagguca ucgacucuau ggaugccgca     1920

uggagcuuuc gagagaugaa agacuacgug cucaagcaaa aagggcuggg caagaaaaag     1980

cgggauuauu uacugacaac cgaaaacauu gacaagaucg agguuaaaaa gaaauucauu     2040

gagcguaauc uaguagauac uagauacgcc ucaagggugg uccucaacag ucugcagucc     2100

gcccuucgcg aauugggaaa ggacaccaaa gugagcgucg ugcggggcca guuuacaucu     2160

cagcugagaa ggaaguggaa aaucgauaag ucacgagaga ccuaucacca ucacgcuguu     2220

gacgcacuca uaauugcggc uaguagccaa cugaaguuau gggaaaaaca ggauaauccc     2280

auguucgugg acuacgggaa caaucaggua gucgauaagc agacuggaga gauccugucc     2340

gugucugacg acgaguauaa agaacuuguu uuucaaccuc cauaccaggg uuucgugaac     2400

acaauuucga gcaagggcuu cgaggaugaa aucuuguuuu ccuaucaggu cgacucaaaa     2460

uacaaucgca aggugaguga ugccacgaua uacucuaccc ggaaggcaaa aaucgggaag     2520

gacaaaaagg aggaaacuua uguacucgga aaaauuaagg auaucuacag ccagaacggc     2580

uucgacacau uugugaagaa auauaacaag gauaaaaccc aauuccugau guaccagaag     2640

gacccccuaa caugggagaa uguuauugag gucauccuga gagauuaucc gacuaccaaa     2700

aaguccgaag acgguaagaa cgaugugaaa uguaauccuu uugaggaaua caggcgugag     2760

aacgggcuca uaugcaagua uaguaaaaag ggcaaaggaa cgcccauuaa gagccugaag     2820

uacuacgaca aaaaguuggg caauuguauc gacaucaccc cacaggaauc uaaaaaccgc     2880

gugguccuuc ggcaaauuuc cccuuggaga gccgauaucu auuucaaucc cgagacacug     2940

aaguacgagu uaauggggcu caaauauagu gaccugagcu uugaaaaggg aacugguaaa     3000

uaccacauuu cucaggagaa guaugaugcu auaaaggaaa aagagggcgu ugggaaggac     3060

uccgaguuca aauuuacccu uuacaggaac gaucugaucu ugauuaagga cacacucaau     3120

aacugcgaac gaaugcugcg guucggaagc aaaaaugaua cuucaaagca uuauguggag     3180

cuaaagccac uggaaaaagg caccuuugac ucagaggaag agauccuccc cgugcugggu     3240

aagguagcca aaucggggca guucauuaag ggccuuaaca aaccuaauau cuccauauac     3300

aagguccgca cggauguguu gggaaacaag uacuuuguua aaaaggaggg cgacaaacca     3360

aagcuggauu ucaaaaauaa caagaaguga                                      3390


<210>  2817
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2817
augagcaacg gcaagauccu ggggcucgac auuggagugg ccuccgucgg uguuggcaua       60

aucgauucua aaaccgggaa ugugauucac gcuaacucaa gacuuuucag ugcagccaau      120

gcggagaaca augcugaaag gcggggauuu cgcggcgccc gacgucugac aagaaggaag      180

aaacaucggg uaaagcgcgu gagagacuug uucgagaaau acgauaucag cacugacuuu      240

aggaacuuaa aucugaaccc cuaugaacua cgggucaagg gucucacgga gcagcugacc      300

aaugaggaac uuuucgcagc ccugcgcaca auugcuaagc gaagagggau cuccuacuug      360

gaugacgcag aggaugacuc uacuggaucg ucagauuaug ccaaaagcau agacgaaaac      420

aggcggcucc ugaagaccaa aacaccuggc caaauccagc uggagcgucu cgaaaaguac      480

ggccaguuac gcgggaauuu uaccguguau gaugagaacg gagaggcuca cagacugauu      540

aauguuuuca guacuuccga cuacaaaaac gaagccagga agauccuuga gacacagucu      600

aauuauaaca agcaaauuac cgaugaauuu aucgaggacu acauagaaau uuugacgcag      660

aaacggaagu acuaucaugg uccaggcaac gagaaaagcc gaacugacua cgggcgcuuc      720

agaaccgaug gaacaacucu ggagaauauc uuuggcauuc uaaucgguaa gugcuccuuc      780

uauccggaag aguacagggc gucaaaagca aguuauaccg cccaggaauu uaacuuccuc      840

aaugaccuga acaaucuuaa ggugcccaca gagaccggga aacugagcac ggaacagaag      900

gaguacuugg ucgauuuugc uaagaaaucu aaggcccucg gagcauccaa acuguuaaag      960

gagaucgcua aaauugugga cuguucagua gaugacauaa agggcuaucg gguugauaac     1020

aaggacaaac cugaucugca cacuuucgaa ccauaccgca agcucaaauu caaucuuagc     1080

aguaucgaca uugaugagcu gucuagagaa acauuggaca agcuagccga cauccugacc     1140

cucaacacug agagggaagg cauagaggau gcgauuaaac guaaucugcc cucccaauuu     1200

acagaggaac agaucagcga gaucgugcag auucggaaga accagucguc ugccuucaau     1260

aagggguggc auuccuuuuc agcaaaacuu augaacgaac ugaucccuga guuguacguc     1320

accagugagg aacaaaugac gauucucacc cgacuggaga aguucaaagu gaauaagaaa     1380

agcuccaaga acacaaagac uauagacgaa aaagagauca ccgaugaaau uuauaaucca     1440

guggucgcua agucuguuag acagacaauc aaaaucauua acgccgcugu gaagaaauac     1500

ggagacuuug auaagauagu aaucgagaug cccagggaca aaaacgccga ggaugaaaag     1560

aaguucauug acaaaaagca gaaagagaau aagaaagaaa aggaugacag cuuaaagcgc     1620

gcagccuuuc uguauaacgg uacugauaau cuaccugacg gcguguucca cgggaacaaa     1680

gagcucaaga ccaaaauccg gcugugguac caacagggag aaagaugccu uuauucaggc     1740

aaguugauua guauccacga ucugguccau aauuccaaca aauuugagau agaccacauc     1800

cucccacuga gccuuucuuu cgacgauuca cuggcuaaua agguguuggu uuacgcgugg     1860

acaaaccagg agaaggguca gaaaaccccg uaucaaguga uugacaguau ggauacugca     1920

uggagcuuua gggaaaugaa ggacuacguc cucaaacaga aggggcuggg caaaaagaag     1980

ugugaguacu uacugacgac agaaaauauc gauaaaauug aggugaagaa aaaguucauc     2040

gaacgcaacc uaguagacac ccgauaugcc ucccggguug ugcucaauuc ucugcagacu     2100

gcucuuaaag aguugggaaa ggauaccaaa gucucggugg uccguggcca guuuacaagc     2160

caacugagaa ggaaguggaa cauagacaag ucccgcgaga ccuaccauca ccacgccgug     2220

gaugcacuca uuaucgccgc uucaagucag cugaaauuau ggcagaagca ggaaaauccc     2280

auguucgagu cuuaugggga aaaccaaguu gugaauaaag agacuggaga gauucugagc     2340

aucuccgacg auaaguacaa agaacuugua uuccagccuc cauaucaggg uuuugucaac     2400

acaauaucaa gcaagggcuu cgaggacgaa aucuuguuuu cuuaccaggu ggauuccaag     2460

uucaaccgga aaguuaguga cgccacgauu uauagcacca gaaaggcaaa acucgggaag     2520

gacaaaaagg augagacuua cgugcuggga aagaucaaag acauuuacuc ucaagauggc     2580

uuugacacau ucaucaagag guauaaaaag gauaaaaccc aguuucuaau guaccagaag     2640

gacccccuga caugggaaaa ugucauagag gugauucucc gagauuaucc uuccgagaag     2700

cugucagaag acgguaaaaa gacuguaaaa ugcaacccau ucgaggaaua ccgccgggag     2760

aaugggcuua ucuguaagua uaguaaaaag ggcaacggaa cccccaucaa gagcuugaaa     2820

uacuaugaua agaaacuggg caauugcauu gacaucacgc cggaaaaguc gaaaaacaga     2880

gugguuuuaa ggcaaauuuc cccuuggcgu gcggauauau acuuuaaucu cgagacccug     2940

aaguacgagc uuauggggcu gaaauauucu gacuugagcu ucgaaaaggg aacagguaag     3000

uaccauaucu cacaggagaa auaugacgcu auucgcgaaa aggagggcau cgggaaaaag     3060

agugaauuua aauucacucu cuaccggaac gaucugauuc uaaucaagga cacccugaau     3120

aacugugaga gaaugcucag guuuggaucc aagaaugaua caucuaaaca cuaugucgag     3180

cugaagcccc uugaaaaagg cacuuucgac agcgaggaag agauauugcc agugcugggu     3240

aagguggcca aauccgggca guucaucaag ggcuuaaaca agccuaauau uucaaucuac     3300

aaaguccgaa ccgauguucu cggaaacaag uuuuucauua aaaaggaggg cgacaaaccc     3360

aagcuggauu uuaagaauaa caacaaauga                                      3390


<210>  2818
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2818
augagcaacg gcaagauccu ggggcucgac auuggagugg ccuccgucgg uguuggcaua       60

aucgaugcua aaaccgggaa ugugauucac gcaaacucua gacuuuucuc agccgcgaau      120

gcugagaaca augccgaaag gcggggauuu cgcggcgcac gacgucugac aagaaggaag      180

aaacaucggg uaaagcgcgu gagagacuug uucgagaaau acgauaucag uacugacuuu      240

aggaacuuaa aucugaaccc cuaugaacua cgggucaagg gucucacgga gcagcugacc      300

aaugaggaac uuuucgccgc ucugcgcaca auugcaaagc gaagagggau cagcuacuug      360

gaugacgccg aggaugacuc cacuggaucu ucggauuaug cuaaaucaau agacgaaaac      420

aggcggcucc ugaagaccaa aacaccuggc caaauccagc uggagcgucu cgaaaaguac      480

ggccaguuac gcgggaauuu uaccguguau gaugagaacg gagaggccca cagacugauu      540

aauguuuuca gcacaaguga cuacaaaaac gaagcgagga agauccuuga gacucagucc      600

aauuauaaca agcaaauuac cgaugaauuu aucgaggacu acauagaaau uuugacgcag      660

aaacggaagu acuaucaugg uccaggcaac gagaaaucuc gaacugacua cgggcgcuuc      720

agaaccgaug gaacaacucu ggagaauauc uuuggcauuc uaaucgguaa gugcagcuuc      780

uauccggaag aguacagggc auccaaagcc ucauauaccg cucaggaauu uaacuuccuc      840

aaugaccuga acaaucuuaa ggugcccaca gagaccggga aacugaguac ggaacagaag      900

gaguacuugg ucgauuuugc caagaaaagc aaggcacucg gagcuucuaa acuguuaaag      960

gagaucgcca aaauugugga cuguuccgua gaugacauaa agggcuaucg gguugauaac     1020

aaggacaaac cugaucugca cacuuucgaa ccauaccgca agcucaaauu caaucuuuca     1080

agcaucgaca uugaugagcu gaguagagaa acauuggaca agcuagcgga cauccugacc     1140

cucaacacug agagggaagg cauagaggau acaauuaaac guaaucugcc cucucaauuu     1200

accgaggaac agaucuccga gaucgugcag auucggaaga accagagcuc ggccuucaau     1260

aagggguggc auucuuuuuc cgcaaaacuu augaacgaac ugaucccuga guuguacguc     1320

acgucagagg aacaaaugac cauucucaca cgacuggaga aguucaaagu gaauaagaaa     1380

aguagcaaga acacuaagac cauagacgaa aaagagauca cagaugaaau uuauaaucca     1440

guggucgcua aguccguuag acagacuauc aaaaucauua acgccgcugu gaagaaauac     1500

ggagacuuug auaagauagu aaucgagaug cccagggaca aaaacgccga ggaugaaaag     1560

aaguucauug acaaaaagga gaaagaaaau aagaaagaga aggaugacuc uuuaaagcgc     1620

gcagccuuuc uguauaacgg uaccgauaau cuaccugacg gcguguucca cgggaacaaa     1680

gaacucaaga caaaaauccg gcugugguac cagcaaggag agagaugccu uuauagcggc     1740

aaguugauuu caauccacga ucugguccau aauaguaaca aauuugagau agaccacauc     1800

cucccacugu cccuuagcuu cgacgauucu cuggcuaaua agguguuggu uuacgcgugg     1860

accaaccagg aaaaggguca gaaaacuccg uaucagguga uugacucaau ggaugcagcc     1920

uggaguuuua gggagaugaa ggacuacguc cucaaacaaa aggggaucgg caaaaagaag     1980

cgcgaauacc uguuaacgac agagaauauu gauaaaaucg aagugaagaa aaaguucaua     2040

gagcgaaacc ugguagacac ccgguaugcu agccguguug ugcuaaauuc ccuccagacu     2100

gcccugaaag agcuuggaaa ggauaccaaa gucucugugg ucagaggcca guuuacaucg     2160

caguugaggc gcaaguggaa gauugacaaa agccgggaaa ccuaccauca ccacgcagug     2220

gaugcccuga ucauugcugc cuccgggcaa cucaagcugu ggcagaaaca ugagaacuua     2280

auguucgaaa auuauggaga gaaccagguu gugaauaagg agacugguga aauccuguca     2340

auaagugacg augaguacaa agaacuugua uuccagcccc cuuaucaagg cuuugucaac     2400

acaaucucua gcaagacguu cgaggacgaa auuuuguuuu ccuaccaggu ggauucaaag     2460

uucaacagaa aaguuagcga cgcaaccauc uauucuacua ggaaggcgaa acucgggaag     2520

gacaaaaagg aggagacaua cgugcuggga aagauuaaag auaucuacac ccaggacggc     2580

uuugauacau ucauaaagcg auauaaaaag gacaaaacuc aguuucuaau guaccaaaag     2640

gauccacuga ccugggaaaa ugucauugag gugauccucc gcgacuaucc cuccgaaaag     2700

cugagugagg augguaaaaa gacgguaaaa uguaacccuu ucgaagagua ccggagagag     2760

aaugggcuua ucugcaagua uagcaaaaag ggcaacggaa ccccaauuaa gucuuugaaa     2820

uacuaugaca agaaacuggg caauuguauc gauauuacac ccgaaaaguc caaaaacagg     2880

gugguuuuac gucagauauc accguggcgc gcugacaucu acuuuaaucu cgagacucug     2940

aaguacgaac uuauggggcu gaaauauagu gauuugagcu ucgagaaggg aaccgguaag     3000

uaccacauuu cgcaggaaaa auaugacgcc auccgggaga aggagggcau ugggaaaaag     3060

uccgaauuua aauucacacu cuacagaaac gaccugaucc uaauaaagga uacucugaau     3120

aacugcgaga ggaugcuccg auuuggaucu aagaaugaca ccagcaaaca uuaugucgaa     3180

cugaagccuc uugagaaagg cacguucgau ucagaggaag agaucuugcc cgugcugggu     3240

aagguggcaa aaagugggca auucauuaag ggcuuaaaca agccaaauau cuccauuuac     3300

aaaguccgga cagacguucu cggaaacaag uuuuucauca aaaaggaagg cgauaaaccu     3360

aagcuggacu uuaagaauaa caacaaauga                                      3390


<210>  2819
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2819
augaacaaga uccugggccu cgaccuuggg accaauagca uuggaugggc ccugauagag       60

aacaauauca uuuuggguau gggcuccaga guguuccccg aaggggucgu uaacuuagga      120

gagggcgaag guagggagac aucuaaaaau gcuucacgga cugaagcacg cggggugcga      180

cgucagaucu uuagaaggcg gcugcgcaag agauaccuac ucaaagagcu ggccaagcac      240

aaccuuugcc cuauuagucc aacgcugauc aaagauugga auggaaccga cauauucaag      300

aacgaguuga ucauucaaug guuucagcuc aauccguaug aacugagggc gaaggcuauc      360

caggaggaaa uuacacugau cgagcucggc cggauauucu accauaugau ucagcgccga      420

ggcuuuuuaa gcaacuccag aucugccggg gcagauguaa aagagacuuc gaccaucuuc      480

aagggagacg ccaaaauugg uaagaucggc aucucagaua cacugaaaag cauugaacaa      540

aauaagaccc uugggaguuu ucuacacucc auacagccca aggagaacga accuuucucu      600

ggagagcugg aaaggauccg gaaucguuau acuacacgcc agauguacau ugacgaguuu      660

gagaccaucu gggaguucca gaaacaauau cauagcgaac uaaacuccaa ucucaagacg      720

auacugggcg guagaaagaa ggauaacuac aaagaagacg gggugcuuuu ucaccagagg      780

ccacugcggu cacagaagca uuuggucgga uucuguaguu uugagcccaa aaagacuaag      840

ugcaaacuca gcgcuauucc uuucgaacug uuccgaaucu aucagugggu gaacaccuua      900

ucuuacgaug uuaauggcgu gcgccaaaag aucacacagg aggagaggaa aaagauuauc      960

gaccugcucc uuuccaaaga aaagccauca uuuaaggaga uuagaaaagc aauaggcaag     1020

cuggauggga gcuaccaguu caacuauaaa gacgaugaca agaucgucgg aagucacacu     1080

auuucuaauu uguccaacaa aaaguuuuuc gguaauaagu gguuugacuu cagcgaaaaa     1140

gagcaagaaa acaucuggca cauccuaucg uuuuucgaug accgggagaa gcugaaagag     1200

uacgccauua agaauugguc uuuugaugag gaacgcgcug ugaaaauauc caaguucaac     1260

cucaaggacg gcuauucaaa ucugaguaga aaagccaucc uuaacauucu gcccuuuuug     1320

cagaaggggu ucauguacga uguagcgguu ggacucgcag gcgugaaaaa uacccugagc     1380

gucaacuggg aggaacauga gcaguuugug uuagacaaug ugccugaaau cgucaggucc     1440

aaccugaagg guggguauau ugagccauug aaaaauaugc ucaagaacga guucaacugu     1500

aaugauaaac agcuggaaaa acuuuaccac cauucuagcg ccaucgaaac agagaacaua     1560

uuggaccguc ugccgguuaa ucuccacgcu gaucgggaga uccaaaagau uaaaaacccc     1620

gugguaauca ccgcccuguu cgaaauucga aaacuuguga augaggucau cgacacguau     1680

ggcaaaccua acgaaauaaa gguggagcug gcaagagauu ugaaggcuuc aaagaguaau     1740

aggcucgaag cccgcaagcg gcagaaagac cuggagaagc agaacgacag aguuaaauua     1800

gagcuggaaa aucuaaacca gagguccaau cacauuaaca uccucaagua caaacugugg     1860

gaggaaugca aucauacuug uccauuuaca ggacaaauua ucaccauaag ccagcuuuuc     1920

acuggcgagg ugcagaucga acacauuuuu cccuggucuc gcucauugaa cgauaguuuc     1980

aauaacaaga cacugugcuu ugcggacgag aaccgagcca aggggaauaa aaccccuuac     2040

gagcucuaua gcgaacaggg agaucaaaag ugggaggcaa ucaaacugca ggcuuuaucc     2100

uguuucaaga cgaaagccaa cuacccaaau ugcuauaaca aguuuaagca uuucgucaaa     2160

aagaaacacg acgaagauuu uauuucucgg cagcugaaug acacccguua caucucgaag     2220

gaggcuaaaa acuaucuuag caagauaugu uccaaugugu ugguagcccc cggucagaug     2280

acagcaacuc ucagacauca cuggggccug aacucaauuc uaaaucugga ggaugacacc     2340

aaaacaaggg augaccaccg ccaucacgcc aucgaugcuc ucguuauggc gugcaguacu     2400

cggucucauc ugcaagaacu uagcaagaga aacagguaca auaagaacua ugacuugaaa     2460

gauuucccgg acccuuggca gaauuuucga caggacgcag agaacgccgu ggcucaaauc     2520

cuggucuccc acaagaaauc aaagagcuua cucaccgugc gcacacacaa aaccauuaag     2580

aagggggucg aacauaaaaa uaucggagug gccgcacggg gccagcugca caaggagucu     2640

guuuacgguc uuagaaaagc ccccaacucc aacaaggcuu uccauauuag gaaaccacug     2700

gaaaguuuga cuacggagaa gcagcucgag aagauagugg augacagcau ccgucugcua     2760

auucugaaac gcaucaauau uaucgggggc uuuauaaagg gaaaaauccc uaaggaaaca     2820

uucuucguag ucgaugagaa cggcauuaaa cagccccaaa ucuuucuccc aaaucggaac     2880

gggaaucccg ugccuauuaa gaagguuaga aucagggaaa acauaggagg ugccgagaaa     2940

cugaaggaaa auaccaacca guacgugaau ccacgaucua accaccaugu cauuguguau     3000

gaggacucca auggcgggcu uaaagaugac guagugacuu ucuggaccgc aguugagcgg     3060

aagcgccagg gacaggaugu cuuuaaauug ccgaacgacg gcgaaaagau cgugucaaca     3120

cugcaaauca augauauguu cuuacucggu cugaacgacg augacauuau aacccuuaca     3180

aguaaggagc ugaaaaagca ccuauuuaaa gugcagacuc ucagcucguc cuucuacgaa     3240

uuuagacugu cuacggauag cacccagaau aagaacacua auucaacagu cuucaggcgg     3300

auccagaguu uugggauggg caaaaccgga uggaaggcgu ucaaccccau uaaaguuaag     3360

uugaacucca ucggcgaggu gaagauuauc uga                                  3393


<210>  2820
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2820
augaccaacg gcaagauccu ggggcucgac auuggaauag ccagcguggg ugucggcauc       60

auugaggcua aaacagggaa gguugugcac gcaaauucca gacuuuucuc ugccgcgaac      120

gcugaaaaua acgccgagag gcggggauuu cgcggcucac gacgucugaa uagaaggaaa      180

aagcaucggg uaaaacgcgu gagagauuug uucgaaaagu acgguaucgu cacugacuuu      240

aggaacuuaa aucugaaccc cuaugagcua cgggugaagg ggcucacgga acagcugacc      300

aaugaggagc uuuucgcagc ccugcgcaca auuaguaaac gaagaggaau cagcuacuug      360

gaugacgcug aagaugacuc cacuggcucu accgauuaug caaagucgau agacgagaac      420

aggcggcucc ugaaaacaaa gaccccuggc caaauccagc uggaacgucu cgagaaauac      480

gggcaguuac gcggaaauuu uacuguuuau gaugagaacg gugaagccca cagacugauu      540

aauguguucu caacaagcga cuacgagaag gaagcuagga agauccuuga gacccaggcc      600

gauuauaaca aaaagauuac ggcggaauuu aucgacgauu acgucgagau auugacucaa      660

aaacggaagu acuaucaugg cccagggaau gagaaaaguc gaaccgacua cggacgcuuc      720

agaacagacg gcacuacccu ggaaaacauu uuugguaucc uaauugggaa gugcuccuuc      780

uauccggaug aguacagggc aucuaaagcc agcuauacag cucaggaaua caacuuucuc      840

aaugaccuga acaaucuuaa ggugcccacc gagacgggaa agcuguccac ugaacagaaa      900

gaggccuugg uagaguucgc aaagucaaca gcuacccucg gcccugccaa acuguuaaag      960

gaaaucgcga aaauccugga uuguaagguu gacgagauua agggcuaucg ggaagaugac     1020

aaagggaagc cagaucucca cacuuuugag cccuaccgca aacuuaaguu caaccuggac     1080

agugugaaua uagaugacuu gagcagagaa guccuagaua aacuggccga cauccucaca     1140

cugaacaccg agagggaggg aauugaagac gcaaucaagc guaaucuucc uaaccaguuc     1200

acggaggaac aaauaucuga gauuaucaau gugcggaagu cccagucaac cgcuuuuaac     1260

aaagguuggc auagcuucag ugccaagcug augaaugagu ugaucccaga acucuacgcu     1320

acaucugaug agcagaugac uauucugacc cgauuagaaa aauuuaaggu gaaaaagaag     1380

uccagcaaaa acaucaagac aauugacgag aaagaaauaa cugaugagau cuauaauccc     1440

gucguugcca agucggugag acagaccauu aaaaucauca acgcagccgu aaagaaauac     1500

ggcgacuucg auaagauugu gauagagaug ccuagggaca agaaugcuga ugacgaaaag     1560

aaguuuaucg auaaacgcaa caaggagaac aaaaaggaaa aggacgaugc gcugaaacgg     1620

gcagccuauc uauacaaugg gacagacaag cucccagaug aggucuucca cggaaacaaa     1680

caacuggaaa ccaagauuag acuuugguau cagcagggcg agaggugcuu guacucuggu     1740

aaaccgaucc ccauucaaga gcuggugcac aauuccaaca auuuugaaau cgaccauaua     1800

cucccucugu cacuuaguuu cgacgauagc cuggcuaaca agguuuuggu guauuccugg     1860

acuaaucagg agaaggggca gcgcacgcca uaccagguca ucgacucuau ggaugccgca     1920

uggagcuuuc gagaaaugaa agacuacgug cucaagcaaa aaggccuggg aaagaaaaag     1980

cgggauuauu uacugacaac cgagaacauu gacaagaucg aaguuaaaaa gaaauucauu     2040

gagcguaauc uaguagauac uagauacgcc ucaagggugg uccucaacag ucugcagucc     2100

gcccuucgcg aguugggcaa ggacaccaaa gugagcgucg ugcgggggca guuuacaucu     2160

cagcugagaa ggaaguggaa aaucgauaag ucacgagaaa ccuaucacca ucacgcuguu     2220

gacgcacuca uaauugcggc uaguagccaa cugaaguuau gggagaaaca ggauaauccc     2280

auguucgugg acuacggaaa caaucaggua gucgauaagc agacugguga aauccugucc     2340

gugucugaca acgaguauaa agagcuuguu uuucaaccuc cauaccaggg cuucgugaau     2400

acaauuucga gcaagggguu cgaagacgag aucuuguuuu ccuaucaggu cgauucaaaa     2460

uacaaccgca aggugaguga cgccacgaua uacucuaccc ggaaggcaaa aaucggaaag     2520

gauaaaaagg aagagacuua uguacucggc aaaauuaagg acaucuacag ccagaacggu     2580

uucgauacau uugugaagaa auauaauaag gacaaaaccc aauuccugau guaccagaag     2640

gauccccuaa caugggaaaa cguuauugag gucauccugg gggacuaucc gacuaccaaa     2700

aaguccgagg auggcaagaa ugacgugaaa uguaacccuu uugaagagua cagaagggaa     2760

aauggacuca uaugcaagua uagcaaaaag ggcaaaggga cgcccauuaa gucacugaag     2820

uacuacgaua aaaagcuagg aaacuguauc gacaucaccc cagaggaauc ucguaauaaa     2880

gugauuuugc aguccaucaa cccuuggcgc gccgacgucu auuucaaucc cgagacacug     2940

aaguacgagu uaaugggucu caaauauagu gaucugagcu uugaaaaggg cacugggaac     3000

uaccacauuu cucaagagaa auaugacgcu auaaaggaaa aggagggaau cggcaaaaag     3060

uccgaguuca aauuuacccu uuaccggaau gaucugauuu ugaucaagga cauugccuca     3120

ggugaacagg agaucuauag auuccucagu aggacaaugc caaacguuaa ucauuacgug     3180

gaacugaaac ccuacgauaa ggagaaguuu gacaacgugc aggaacuugu agaggcacug     3240

ggggaggcug auaaagucgg ccgaugcaua aagggacuca auaaaccuaa caucagcauu     3300

uauaaggugc ggacugacgu ucugggcaac aaauacuucg ugaagaagga aggggauaaa     3360

ccaaaacuug acuuuaaaaa uaacaagaaa uga                                  3393


<210>  2821
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2821
augaccaacg gcaagauccu ggggcucgac auuggaauag ccagcguggg ugucggcauc       60

auugaggcua aaacagggaa gguugugcac gcaaauucca gacuuuucuc ugccgcgaac      120

gcugaaaaua acgccgagag gcggggauuu cgcggcucac gacgucugaa uagaaggaaa      180

aagcaucggg uaaaacgcgu gagagauuug uucgaaaagu acgagaucgu cacugacuuu      240

aggaacuuaa aucugaaccc cuaugaacua cgggugaagg gucucacgga gcagcugacc      300

aaugaggaac uuuucgcagc ccugcgcaca auuaguaaac gaagagggau cagcuacuug      360

gaugacgcug aggaugacuc cacuggaucu accgauuaug caaagucgau agacgaaaac      420

aggcggcucc ugaaaacaaa gaccccuggc caaauccagc uggagcgucu cgagaaauac      480

ggccaguuac gcgggaauuu uacuguuuau gaugaaaacg gagaggccca cagacugauu      540

aauguguucu caacaagcga cuacgaaaag gaggcuagga agauccuuga aacccaggcc      600

gauuauaaca aaaagauuac ggcggaguuu aucgacgauu acgucgagau auugacucaa      660

aaacggaagu acuaucaugg uccaggcaau gagaaaaguc gaaccgacua cgggcgcuuc      720

agaacagacg gaacuacccu ggaaaacauu uuuggcaucc uaauugguaa gugcuccuuc      780

uauccggaug aauacagggc aucuaaagcc agcuauacag cucaggagua caacuuucuc      840

aaugaccuga acaaucuuaa ggugcccacc gaaacgggga agcuguccac ugagcagaaa      900

gaggccuugg uagaguucgc aaagucaaca gcuacccucg gaccugccaa acuguuaaag      960

gaaaucgcga aaauccugga uuguaagguu gacgaaauua agggcuaucg ggaggaugac     1020

aaaggcaagc cagaucucca cacuuuugaa cccuaccgca aacuuaaguu caaccuggac     1080

agugugaaua uagaugacuu gagcagagag guccuagaua aacuggccga cauccucaca     1140

cugaacaccg agagggaagg gauugaggac gcaauccguc auaaucuucc uaaccaguuc     1200

acggaaggac aaauaucuga gauuaucaag gugcggaagu cccagucaac cgcuuuuaau     1260

aaagguuggc acagcuucag ugccaagcug augaacgagu ugaucccaga acucuacgcu     1320

acaucugaug agcagaugac uauucugacc cgauuagaaa aauuuaaggu gaauaaaaag     1380

uccagcaaga acacaaaaac uaucgacgag aaggaaguca ccgaugagau uuauaauccc     1440

guuguggcca aaucgguaag acagacaaua aagaucauua acgcagccgu gaaaaaguac     1500

ggcgacuucg auaaaaucgu caucgagaug ccuagggaca agaaugcuga ugacgaaaag     1560

aaauuuauug auaagcgcaa caaagagaac aagaaagaaa aggacgaugc gcugaagcgg     1620

gcagccuauc uauacaaugg gaccgacaaa cucccagaug agguguucca cggaaacaag     1680

caacuggaaa cuaaaauaag acuuugguau cagcagggcg agaggugcuu guacucuggu     1740

aagccgaucc ccauucaaga gcugguucau aauuccaaca auuuugaaau cgaccacauu     1800

cucccucugu cacuuaguuu cgacgauagc cuggcuaaca aaguguuggu cuaugccugg     1860

acgaaucagg agaaggggca gaagacacca uaccagguga ucgacuccau ggaugcagcc     1920

uggucuuuuc gcgaaaugaa agacuacgua cucaagcaaa aaggccuggg aaagaaaaag     1980

cgagauuauu uacugaccac ugagaacaua gacaagaucg aaguuaaaaa gaaauucauu     2040

gagcggaauc uaguggauac ccguuacgcu agcagagucg ugcucaacuc acugcagagu     2100

gcccuuaggg aguugggcaa ggacacaaaa aucuccguca uucgcgggca guuuaccagc     2160

cagcugcgga gaaaguggaa aaucgauaag ucuagggaaa cuuaucauca ccacgcagug     2220

gacgcgcuca uaauugcugc cucaagucaa cugaaguuau gggagaaaca ggauaauccc     2280

auguucguug acuacggaaa caaucaggug guagauaagc agacagguga aauccugagc     2340

gucuccgacg acgaguauaa agagcuugug uuucaaccuc cauaccaggg cuucguuaac     2400

augauuucuu cgaagggguu cgaagaugag aucuuguuua gcuaucaggu ggacuccaaa     2460

uacaaucgaa aggucucaga ugcaacgaua uacaguaccc gcaaggccaa aaucggaaag     2520

gacaaaaagg aagagacuua ugugcucggc aaaauuaagg auaucuacuc ucagaacggu     2580

uucgacacau uuauuaagaa auauaacaag gauaaaaccc aauuccugau guaccagaag     2640

gacccccuaa caugggaaaa uguaaucgag gugauacugc gggauuaucc gacuaccaaa     2700

aagagcgagg acgggaagaa cgauguuaaa uguaauccuu uugaagagua cagaagggaa     2760

aacggccucg ucugcaagua uuccaaaaag ggaaaaggca cgcccauuaa gucacugaag     2820

uacuacgaca aaaaguuggg gaauuguauc gacaucaccc cagagggaag caaaaacgaa     2880

guggugcuuc agucucugaa uccuuggcgu gcugaugucu auuucaaccc cgagacauua     2940

aaguacgagc uccugggucu uaaauauucc gaccugaguu uugaaaaggg cacugggaaa     3000

uaccauauua gccaagagaa guaugauguu aucaaggaaa aagagggaau uggcaagaaa     3060

ucugaguuca aguuuaccuu guaccgcaau gaccucauac ugaucaaaga uacagccucc     3120

ggugaacagg agauuuaucg guuccuauca agaacuaugc caaacgugaa gcacuacgca     3180

gaacugaagc ccuacgacaa agagaaguuu gauaaugugc aggaacucgu agaggcucug     3240

ggggaggccg acaaagucgg aaggugcauc aagggccuua acaaaaguaa uuugagcauu     3300

uauaaggugc gaaccgaugu ucugggcaac aaguacuucg ugaaaaagga aggggacaaa     3360

ccuaaguuag auuuuaaaaa uaacaagaag uga                                  3393


<210>  2822
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2822
augaccaacg gcaagauccu ggggcucgac auuggaauag ccagcguggg ugucggcauc       60

auugaggcua aaacagggaa gguugugcac gcaaauucca gacuuuucuc ugccgcgaac      120

gcugaaaaua acgccgagag gcggggauuu cgcggcucac gacgucugaa uagaaggaaa      180

aagcaucggg uaaaacgcgu gagagauuug uucgaaaagu acgguaucgu cacugacuuu      240

aggaacuuaa aucugaaccc cuaugagcua cgggugaagg ggcucacgga acagcugacc      300

aaugaggagc uuuucgcagc ccugcgcaca auuaguaaac gaagaggaau cagcuacuug      360

gaugacgcug aagaugacuc cacuggcucu accgauuaug caaagucgau agacgagaac      420

aggcggcucc ugaaaacaaa gaccccuggc caaauccagc uggaacgucu cgagaaauac      480

gggcaguuac gcggaaauuu uacuguuuau gaugagaacg gugaagccca cagacugauu      540

aauguguucu caacaagcga cuacgagaag gaagcuagga agauccuuga gacccaggcc      600

gauuauaaca aaaagauuac ggcggaauuu aucgacgauu acgucgagau auugacucaa      660

aaacggaagu acuaucaugg cccagggaau gagaaaaguc gaaccgacua cggacgcuuc      720

agaacagacg gcacuacccu ggaaaacauu uuugguaucc uaauugggaa gugcaacuuc      780

uauccggaug aguacagggc auccaaagcc ucuuauacag cucaggaaua caauuuucuc      840

aacgaccuga auaaccuuaa ggugcccacc gagacgggaa agcugagcac ugaacagaaa      900

gaguccuugg uagaguucgc caagaauaca gcaauccucg gcccugcuaa acuguuaaag      960

gaaaucgcca aaauucugga uuguaagguu gacgagauaa agggcuaucg ggaagaugac     1020

aaagggaagc cagaucucca caccuuugag cccuaccgca aacuuaaguu caaccuggac     1080

ucagugaaua ucgauuacuu gaguagagaa guccuagaca aacuggcgga uauucucacu     1140

cugaacacag agagggaggg aaucgaagac gccauaaagc guaaucuucc uaaccaguuc     1200

accgaggaac aaauuagcga gaucaucaau gugcggaagu cucaguccac ggcauuuaac     1260

aaagguuggc auucauucag cgcuaagcug augaaugagu ugauuccaga acucuaugcc     1320

accagugacg agcagaugac aauccugacu cgauuagaaa aauuuaaggu gaaaaagaag     1380

ucuuccaaaa acauuaagac cauagaugag aaagaaauca cagacgagau uuacaauccc     1440

gucguugcua agagcgugag acagacuauc aaaaucauua acgccgcagu aaagaaauau     1500

ggcgauuucg acaagauagu gaucgagaug ccuagggaua agaacgccga cgaugaaaag     1560

aaguuuauua auaaacgcaa caaggagaau aaaaaggaaa aggacgaugc ucugaaacgg     1620

gcggcauacc uauauaacgg gaccgacaag cucccagaug aggucuucca cggaaauaaa     1680

caacuggaaa caaagaucag acuuugguac cagcagggcg agaggugcuu guauucgggu     1740

aaaccgauuc ccauccaaga gcuggugcac aacucuaaua acuuugaaau agaccauauc     1800

cucccucugu cccuuucauu cgaugacagu cuggccaaua agguuuuggu guacagcugg     1860

accaaccagg agaaggggca gaaaacucca uaccagguca uugacuccau ggaugcugcc     1920

uggucuuuuc gcgaaaugaa ggaguaugug cucaaacaaa agggccuggg caaaaagaag     1980

cgagacuacu uacugacgac agaaaauauc gauaaaauug agguaaagaa aaaguucauc     2040

gagcggaacc uaguugacac ccguuaugca agcagagugg uccucaauuc acugcagagu     2100

gcccuuaggg aauugggcaa agauacuaag guguccgucg ugcgcgggca guuuaccagc     2160

cagcugcgga gaaaauggaa gauagacaag ucuagggaga cauaccacca ucacgcuguu     2220

gaugcccuca uuaucgcagc gucaagucaa cugaaauuau gggaaaagca ggacaacccc     2280

auguucgugg auuauggaaa uaaccaggua gucgacaaac agaccgguga gauucugagc     2340

guguccgaua acgaguacaa ggaacuuguu uuucaaccuc cauaucaggg cuucgugaau     2400

acuaucucuu cgaaaggguu cgaggacgaa auauuguuua gcuaccaggu cgauuccaag     2460

uacaaccgaa aggugucaga cgcuacaauc uauaguacgc gcaaagccaa gauuggaaaa     2520

gacaagaaag aggaaaccua cguacucggc aagaucaagg auauuuauuc ucagaauggu     2580

uucgacacuu uugugaaaaa guacaacaaa gauaagacac aauuccugau guaucagaaa     2640

gacccccuaa ccugggagaa uguuaucgag gucauacugg gggauuaccc gacaacuaaa     2700

aagagcgaag acggcaaaaa cgaugugaag uguaauccuu uugaggaaua ucggagagag     2760

aacggacuca uuugcaaaua cuccaagaaa ggcaagggga cccccaucaa gucacugaaa     2820

uacuaugaca agaaacuugg aaauuguauc gauauuacgc cagaagagag caggaacaag     2880

gugaucuugc agucuauuaa uccuuggcgu gcagacgucu acuucaaccc cgagacccug     2940

aaauaugaau uaaugggucu caaguacucc gaucugaguu uugagaaagg cacagggaau     3000

uaucacauaa gccaagaaaa guacgacgcc aucaaggaga aagaaggaau uggcaagaaa     3060

ucugaguuca aguuuacucu uuaucgcaac gaccugaucu ugauuaaaga uaucgcuucc     3120

ggugagcagg aaauauaccg guuccucuca agaaccaugc caaauguuaa ccauuacgug     3180

gagcugaagc ccuaugacaa ggaaaaauuu gauaacgugc aggagcuagu agaggcccug     3240

ggggaagcag acaaggucgg aaggugcauc aaaagucuca auaagccuaa cauuagcauc     3300

uacaaagugc gaacagaugu ucugggaaau aaguauuucg ugaagaaaga gggcgacaag     3360

ccaaaacuug auuuuaagaa caauaaaaag uga                                  3393


<210>  2823
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2823
augagcaacg gcaagauccu ggggcucgac auuggagugg ccuccgucgg uguuggcaua       60

aucgaugcua aaaccgggaa ggugauucac gcaucuucaa gacuuuucag ugccgcgaau      120

gcugagaaca augccgaaag gcggggauuu cgcggcgcac gacgucugac aagaaggaaa      180

aagcaucgga ucaaacgcgu aagagacuug uucgagaagu acgaaauugu gacugauuuu      240

agcaacuuaa aucugaaccc cuaugagcua agggucaagg gucucacgga acagcugacc      300

aaugaggagc uuuucgccgc ucugcggaca aucgcaaaac gccgagggau auccuacuug      360

gacgaugccg aagacgauuc uacuggaucg ucagacuaug cuaagagcau cgaggaaaac      420

agaaggcucc ugaaaaauau gaccccuggc caaauucagc uggagcggcu cgagaaguac      480

ggccaguuac gugggaacuu uacaguguau gaugaaaaug gagaggccca ccgccugauc      540

aacguuuuca guaccuccga cuacaaaaau gaagcgagaa agauucuuga gacucagucu      600

aacuauaaua agcaaaucac agaugaauuu auagaggacu acauugagau cuugacccag      660

aaaaggaagu acuaucaugg uccaggcaac gaaaagagcc ggacggauua cgggcgauau      720

cgcacugacg gaaccacacu ggagaacauu uucggcaucc uaaucgguaa gugcacuuuu      780

uacccggacg aauauagaac cuccaaagca ucauacacag cccaggagua uaauuuccuc      840

aacgaucuga auaaccuuaa ggugcccacc gaaacgggga aacugaguac ugagcagaag      900

cacuacuugg ucgacuuugc uaagagcucu aaagcccucg gaguggcaaa gcuguuaaaa      960

gagauugcua agauaguaga auguuccguu gaugacauca aaggcuacag ggaggauaau     1020

aagggcaagc cugaccugca uacauucgaa ccauaucgga aacucaaguu uaaccuuuca     1080

cugauugaua ucgacgaguu gagccgcgaa acccuagaua aacuggccga cauacucacu     1140

cugaauacag agagagaggg gauugaagau gcgaucaaga ggaaccuucc caaucaauuc     1200

accgaggaac agaucgugga gcuggucaac gugcguaaaa aucagaguuc ugccuucaac     1260

aagggauggc acuccuuuag cgugaaguug augaaugagc ucauuccuga acuguacgca     1320

ucgucugagg aacagaugac gaucuuaacc cggcuggaga aauucaaggu caacaaaaag     1380

uccucagaaa auacaaaaac uauuaacgag aaggagauaa ccgacgaaau cuauaaccca     1440

guuguggcua agaguguaag ccaaacagug aaaauuauca augccgcugu caagaaauac     1500

ggugacuuug auaagaucgu gauugagaug ccccgagaca aaaacgccga ugacgaaaag     1560

aaauucauag auaagaagca gaaagagaau gcaaaggaag uugaggacgc ccuaaaaaga     1620

gcugcguauc ucuacaacgg cacugauaag cugccugacg ggguguuuca cggaaauaaa     1680

cagcuuaaga ccaagaucag guugugguau caacagggcg agcgcugccu guacuccggu     1740

aaaccaauuu cuauccagga ucucguccau aacagcaaua acuucgaaau ugaccacauc     1800

cugccgcuuu cacugaguuu ugaugacucc uuggcaaaua aggugcucgu auacgccugg     1860

acaaaccagg agaaagggca aaagaccccc uaucagguua uagauagcau ggacgcugcc     1920

uggucuuucc gggaaaugaa agacuacgug cugaaucaga agggcaucgg aaagaaaaag     1980

agagaguauu uacugacuac ggaaaacauu gauaaaaucg aggucaagaa aaaguuuauu     2040

gagaggaauc uaguggacac acgcuacgca ucacgagucg ugcucaacag uauccaggcc     2100

gcucugaagg aacuugauaa agacaccaag guuagcguga uacggggcca auucacuucc     2160

caguugcgua gaaaauggaa gauugauaaa ucuagggaga ccuaucauca ccacgccgua     2220

gacgcacuga ucauugcggc uucgagccag cucaagcugu ggcagaaaca agaaaauccu     2280

auguuugucg auuacgggga gaaccaggug guuaacaagg agacaggaga aaucuuaucc     2340

gugucagacg augacuauaa ggagcugguc uuccagccac ccuaccaggg uuuugugaau     2400

accauaaguu cuaaagccuu cgaagaugag auccuuuuca gcuaccaagu agacuccaag     2460

uauaaccgca aagugucaga ugcaacuauu uacagcacac ggaaggccaa auugggcaag     2520

gacaagaaag aagagacgua uguucucggg aagaucaaag acauuuacuc ucaggaugga     2580

uuugacgcuu ucaucaagag auauaaaaag gauaagaccc aguuucugau guaccaaaaa     2640

gacccuguca cuugggagaa ugugauagaa gugauucuaa gggauuaucc auccgagaag     2700

cugagugaag acggcaaaaa gaacgucaaa uguaaucccu ucgaggaaua ccgacgcgag     2760

aacggucuca ucugcaagua cagcaagaaa gggaagggca caccgaucaa aucucugaag     2820

uauuacgaua aaaaguuggg aaauuguauu gacaucaccc cuaagggcuc caaaaacgag     2880

guugugcuuc agucacugaa ucccuggcgg gccgauuuau auuuuaaccc agacacacuc     2940

aaguacgaac ugauggggcu uaaauauagu gaucugagcu ucgagaaggg aacugguaaa     3000

uaccauauuu cgcaggaaag auaugacgag auaaagguga aagaaggcau cgggaaaaau     3060

uccgaguuua aguucaccuu guacaggaac gaccucauuc ugaucaaaga uacggagucu     3120

ggagaagagg aacuauaccg uuuucugagc cgcaccaugc cuaauguaaa gcacuauguc     3180

gagcucaaac ccuacgacaa ggagcgguau gauggcgguc aggaacugau gaaaguguuc     3240

gggaacguug caaauggcgg acaaugccuu aagucauuga acaagaguaa ccuguccauu     3300

uacaaaguga gaacagacgu cuuaggcaau aaguuuuuca ucaaaaagga gggggauaaa     3360

ccaaagcucg acuuuaagaa caauaaaaag uga                                  3393


<210>  2824
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2824
augaccaacg gcaagauccu ggggcucgac auuggaauag ccagcguggg ugucggcauc       60

auugaggcua aaacagggaa gguugugcac gcaaauucca gacuuuucuc ugccgcgaac      120

gcugaaaaua acgccgagag gcggggauuu cgcggcucac gacgucugaa uagaaggaaa      180

aagcaucggg uaaaacgcgu gagagauuug uucgaaaagu acgguaucgu cacugacuuu      240

aggaacuuaa aucugaaccc cuaugagcua cgggugaagg ggcucacgga acagcugaaa      300

aaugaggagc uuuucgcagc ccugcgcacc auuaguaagc gaagaggaau cagcuacuug      360

gaugacgcug aagaugacuc cacaggcucu acugauuaug caaaaucgau agacgagaac      420

aggcggcucc ugaagaauaa aaccccuggc caaauccagc uggaacgucu cgagaaguac      480

gggcaguuac gcggaaacuu uacaguuuau gaugagaaug gugaagccca cagacugauu      540

aacguguucu caaccagcga cuacgagaag gaagcuagga aaauccuuga gacucaggcc      600

gauuauaaua agaaaauuac agcggaauuu aucgacgauu acgucgagau auugacccaa      660

aagcggaaau acuaucaugg cccagggaac gagaagaguc gaacggacua cggacgcuuc      720

agaacugacg gcaccacacu ggaaaacauu uuugguaucc uaauugggaa augcaauuuc      780

uauccggaug aguacagggc auccaaggcc ucuuauacug cucaggaaua caacuuucuc      840

aaugaccuga acaaucuuaa ggugagcacc gagacaggaa aacuguccac cgaacagaag      900

gagucauugg uagaguucgc caaaaacacg gcaacucucg gccccgcuaa gcuguuaaaa      960

gaaaucgcca agauccugga uuguaagguu gacgagauua aaggcuaucg ggaagaugac     1020

aaggggaaac cugaucucca cacauuugag ccauaccgca aacuuaaauu caaucuggaa     1080

aguauaaaca ucgacgauuu gagcagagag gugauugaca agcuagcgga uauccugacc     1140

cucaauacug agagggaagg aauagaggac gccauuaagc guaaccugcc caaucaguuc     1200

acagaggaac aaaucucuga gaucauuaaa guccggaagu cccagucaac cgcauuuaac     1260

aaagguuggc auagcuucag ugcuaaacuu augaaugaac ugaucccuga guuguacgcc     1320

acgucugacg aacagaugac cauucucaca cgacuggaga aauuuaaggu gaacaagaaa     1380

uccagcaaga auacuaaaac cauagaugaa aaggagguga cagacgagau cuauaaccca     1440

gucguugcua aaucggugag acagacuauu aagaucauca acgccgcagu aaagaaguac     1500

ggcgauuucg acaagauugu gauagaaaug cccagggaua aaaaugccga cgaugagaag     1560

aaauuuaucg acaagcgcaa caaagaaaau aagaaggaga aagaugacgc uuuaaagcgg     1620

gcggcauauc uguacaacuc uuccgauaaa cuaccugacg aagucuucca cgggaauaag     1680

caacucgaga ccaaaauuag acugugguau cagcagggag agaggugccu uuacucaggc     1740

aagccaauca guauucaaga auuggugcac aacagcaaua acuuugagau cgaucauaua     1800

cugccgcucu cccugucuuu cgacgacagc cuugccaaua agguucuggu guaugcuugg     1860

acaaaccagg aaaaagguca gaagaccccc uaccagguca ucgauucaau ggacgccgca     1920

uggaguuuuc gcgagaugaa agauuacgug uugaagcaaa aagggcucgg caagaagaaa     1980

cgagacuauc uguuaacuac ggaaaauauu gauaagaucg agguaaagaa gaaauucauu     2040

gagcggaacc ugguugacac acguuacgcc uccagagugg uccuaaauag ccuccagucu     2100

gcucugaggg aacuuggaaa ggauaccaaa gugucagucg ugcgcggcca guuuacaagu     2160

caguugcgga gaaaguggaa gaucgacaaa agcagggaga ccuaucacca ucacgccguu     2220

gaugcacuga uaauugcggc uuccucucaa cucaagcugu gggaaaaaca ggacaacccu     2280

auguucgugg auuacgggaa gaaucaggua gucgacaaac agacaggaga gaucuuaucg     2340

gugagcgaug acgaguauaa ggaacugguu uuucaaccac ccuaccaggg uuucgugaac     2400

accauuuccu caaagggcuu cgaggacgaa auccuuuuua guuaucaggu cgauucuaaa     2460

uacaaccgaa aggugagcga cgccacuaua uacuccacac gcaaagcaaa gaucgggaaa     2520

gauaagaagg aggaaacgua uguauuggga aaaauuaagg acaucuacuc acagaauggc     2580

uucgauaccu uuauuaaaaa guauaacaaa gacaagacuc aauuccucau guaccagaag     2640

gauagccuga caugggagaa ugugaucgag guuauacuac gggacuaucc uaccacuaaa     2700

aagucugaag augguaaaaa cgacgucaag uguaauccau uugaggaaua cagaagggag     2760

aacgggcuga uuugcaaaua uuccaagaag ggcaaaggaa cucccaucaa gagucucaaa     2820

uacuacgaua agaaacuggg caauuguauc gacauuaccc cggaagagag ccguaacaag     2880

gugauccuuc agucuauuaa uccuuggcgc gccgacgugu auuucaaccc cgagacguug     2940

aaauacgaac ugaugggguu aaaguauucc gaucucucau uugagaaggg aaccgguaau     3000

uaccacauaa gucaagaaaa auaugacgcu aucaaggaga aagaaggcau ugggaagaaa     3060

agcgaguuca aguuuacacu guaccggaac gaucuuaucc ugauuaagga caucgccucg     3120

ggagagcagg aaauauauag auucuugucc aggacuaugc caaaugucaa ccauuacguu     3180

gagcucaaac cuuacgauaa ggaaaaauuu gacaaugugc aggagcuggu ggaggcacua     3240

ggcgaagcug auaagguagg ucgaugcauc aaagggcuga acaagcccaa cauuucuauc     3300

uauaaggucc ggaccgacgu gcucggcaau aaauacuucg uuaagaaaaa gggagauaaa     3360

ccaaagcugg acuuuaagaa caauaaaaag uga                                  3393


<210>  2825
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2825
augaccaacg gcaagauccu ggggcucgac auuggaauag ccagcguggg ugucggcauc       60

auugaggcua aaacagggaa gguugugcac gcaaauucca gacuuuucuc ugccgcgaac      120

gcugaaaaua acgccgagag gcggggauuu cgcggcucac gacgucugaa uagaaggaaa      180

aagcaucggg uaaaacgcgu gagagauuug uucgaaaagu acgguaucgu cacugacuuu      240

aggaacuuaa aucugaaccc cuaugagcua cgggugaagg ggcucacgga acagcugacc      300

aaugaggagc uuuucgcagc ccugcgcaca auuaguaaac gaagaggaau cagcuacuug      360

gaugacgcug aagaugacuc cacuggcucu accgauuaug caaagucgau agacgagaac      420

aggcggcucc ugaaaacaaa gaccccuggc caaauccagc uggaacgucu cgagaaauac      480

gggcaguuac gcggaaauuu uacuguuuau gaugagaacg gugaagccca cagacugauu      540

aauguguucu caacaagcga cuacgagaag gaagcuagga agauccuuga gacccaggcc      600

gauuauaaca aaaagauuac ggcggaauuu aucgacgauu acgucgagau auugacucaa      660

aaacggaagu acuaucaugg cccagggaau gagaaaaguc gaaccgacua cggacgcuuc      720

agaacagacg gcacuacccu ggaaaacauu uuugguaucc uaauugggaa gugcuccuuc      780

uauccggaug aguacagggc aucuaaagcc agcuauacag cucaggaaua caacuuucuc      840

aaugaccuga acaaucuuaa ggugcccacc gagacgggaa agcuguccac ugaacagaaa      900

gaggccuugg uagaguucgc aaagucaaca gcuacccucg gcccugccaa acuguuaaag      960

gaaaucgcga aaauccugga uuguaagguu gacgagauua agggcuaucg ggaagaugac     1020

aaagggaagc cagaucucca cacuuuugag cccuaccgca aacuuaaguu caaccuggac     1080

agugugaaua uagauuacuu gagcagagaa guccuagaca aacuggccgg aauccucaca     1140

cugaacaccg agagggaggg uauugaagau gcaaucaagc guaaucuucc uaaccaguuc     1200

acggaggaac aaauaucuga gauuaucaau gugcggaagu cccagucaac cgcuuuuaac     1260

aaaggcuggc auagcuucag ugccaagcug augaaugagu ugaucccaga acucuaugcu     1320

acaucugacg agcagaugac uauucugacc cgauuagaaa aauuuaaggu gaaaaagaag     1380

uccagcaaaa acaucaagac aauugacgag aaagaaauaa cugaugagau cuacaauccc     1440

gucguugcca agucggugag acagaccauu aaaaucauca acgcagccgu aaagaaauau     1500

ggggacuucg auaagauugu gauagagaug ccuagggaca agaaugcuga ugacgagaaa     1560

aaguuuauca acaaacgcaa caaggaaaau aaaaaggaaa aggaugacgc gcugaaacgg     1620

gcagccuacc uauauaacgg aacagauaag cucccagacg aggucuucca cggcaauaaa     1680

caacuggaaa ccaagauuag acuuugguac cagcagggug agaggugcuu guauucuggg     1740

aaaccgaucc ccauucaaga gcuggugcac aacuccaaua acuuugaaau cgaucauaua     1800

cucccucugu cacuuaguuu cgacgauagc cuggcuaaua agguuuuggu guacuccugg     1860

acuaaccagg agaagggcca gaaaacgcca uaccagguca ucgacucuau ggacgccgca     1920

uggagcuuuc gcgaaaugaa ggaguaugug cucaaacaaa agggacuggg caaaaagaag     1980

cgagauuacu uacugacaac cgaaaauauu gacaaaaucg agguaaagaa aaaguucauu     2040

gagcggaacc uaguugauac ucguuaugcc ucaagagugg uccucaauag ucugcagucc     2100

gcucuuaggg aauuggggaa agacaccaag gugagcgucg ugcgcggaca guuuacaucu     2160

cagcugcgga gaaaauggaa gaucgauaag ucaagggaga ccuaccacca ucacgccguu     2220

gacgcacuca uaauugcggc uaguagccaa cugaaauuau gggaaaagca ggauaacccc     2280

auguucgugg acuaugguaa uaaccaggua gucgauaaac agacuggcga gauccugucc     2340

gugucugaca augaguacaa ggaacuuguu uuucaaccuc cauaucaggg guucgugaac     2400

acaauuucga gcaaaggauu cgaggaugaa aucuuguuuu ccuaccaggu cgacucaaag     2460

uacaaccgaa aggugaguga ugccacgaua uauucuaccc gcaaagcaaa gaucggcaaa     2520

gacaagaaag aggaaacuua cguacucggu aagauuaagg acaucuauag ccagaauggg     2580

uucgauacau uugugaaaaa guacaacaaa gacaagaccc aauuccugau guaucagaaa     2640

gauccccuaa caugggagaa uguuauugag gucauccugg gcgacuaccc gacuaccaag     2700

aaguccgaag auggcaaaaa cgacgugaag uguaauccuu uugaggaaua ucggagagag     2760

aacggacuca uaugcaaaua cucaaagaaa gggaagggaa cgcccauuaa gagccugaaa     2820

uacuaugaua agaaacuugg uaauuguauc gacaucaccc cagaagaguc uaggaacaag     2880

gugauuuugc aguccaucaa uccuuggcgu gccgaugucu acuucaaccc cgagacacug     2940

aaauaugaau uaaugggccu caaguacagu gaccugagcu uugagaaagg gacuggaaau     3000

uaucacauuu cucaagaaaa guacgaugcu auaaaggaga aagaaggcau cgguaagaaa     3060

uccgaguuca aguuuacccu uuaucgcaac gaccugauuu ugaucaaaga cauugccuca     3120

ggggagcagg aaaucuaccg guuccucagu agaacaaugc caaauguuaa ccauuacgug     3180

gagcugaagc ccuaugauaa ggaaaaauuu gacaaugugc aggagcuagu agaggcacug     3240

ggcgaagcug auaaggucgg aaggugcaua aaaggccuca acaagccuaa caucagcauu     3300

uacaaagugc gaacugacgu ucuggggaau aaguauuucg ugaagaaaga gggagauaag     3360

ccaaaacuug acuuuaagaa caauaaaaag uga                                  3393


<210>  2826
<211>  3396
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2826
auggcccagc acguguucgg ccuggacauc gggauugcua gcgucggaug ggcaauacuc       60

ggugagcaaa gaaucauuga ucuuggcguu aggugcuuug acaaggccga aaccgcgaaa      120

gagggggauc cccugaacuu gacacggcgc caggcucgau uacugcguag aaggcuauac      180

cggcgcgccu ggagacucac ucagcugucc aggcuucuga agcggaaagg auugaucgca      240

gacgccaaac ucuucgcuaa ggcaccuucu uauggcgauu cagccuggga acugcgccga      300

cagggucugg acagacucuu aacgccacug gagugggcua gggugauuua ccaucaaugu      360

aagcaccggg gguuucauug gaccaguaag gccgaagagg cgaaagcaga uagcgacgcc      420

gagggaggcc guguaaagca gggccuugcu cacacaaaag ccuugaugca ggcaaagaau      480

uaucgcuccg cugccgaaau ggugcuggcg gaguucccgg augcccagag aaacaaaagg      540

gggcaauacg acaaggcacu aucucggguc cuccugggag aagaacuugc ucuguuguuu      600

gccacucagc gacgccucgg uaauccccau gcuucggauu ucuuugagaa gcugaucuua      660

ggcgacgggg auagaaaauc aggacuguuc uggcagcaga agccugcccu cagcggcgca      720

gaccuucuga aaauguuggg uaagugcacc uuugaaaaag gggaguauag ggccccaaag      780

gcuaguuucu ccguggaacg gcacguuugg cuaacacgcc ugaacaaucu cagaauagug      840

gucgacggaa ggucucgucc ccugaacgag gcggaacggc aagcagcccu ucuguugccu      900

uaccagaccg agacuagcaa auauaagaca cucaagaaug cuuuuaucaa agccggccug      960

uggggcgaug gggugcgauu cggagguuua gcauacccau cccaggccca aauugacgcu     1020

gagaagacca aagaucccga agaccaguuu cugguaaagc uaccugccug gcacgagcuc     1080

agaaaagcau ucaaggcggc uggccaugaa gcccuguggc agcagaucuc aacgccagca     1140

cuugaugggg acccgacuuu gcuggaucaa auugccaccg uucucagugu guacaaggac     1200

ggagcugagg ucgugcagca gcugaggcag cuugcccugc ccgaaccugc agcuagcauc     1260

gccguguugg agaaaauauc uuucgauaag uuuuccucac ucagccugaa agcguuacgc     1320

cggauugucc cacugaugca aaguggccua agauaugacg aggccguugc ucagaucccc     1380

gaauacgguc accauucuca gaggauugag ccuggggcag ccaagcaccu cuaucugcca     1440

cccuucuacg aagcucagcg caaauaugcc ggcaagggag aucacaucgg cuccaugcaa     1500

uuucgagacg acgcagauau cccgcggaac ccugugguac uucgugcguu gaaucaggcc     1560

agaaaggugg ucaacgcucu gauuagggag uacgggagcc ccauagccgu gaauaucgaa     1620

auggcacgcg accucucgcg gccacuggau gagagaaaca aaguuaagag ggcucaggag     1680

gaguuccgag accgcaauga ucgggccaga ucugaguuug aaagggacuu cggauauaaa     1740

ccuaaggcag ccgcuuuuga gaaauggaug uuauaccgug agcagcuggg ucaaugugcg     1800

uacucccagc agccccuuga uauucaacgc guguuggacg aucauaacua ugcccagguc     1860

gaccacgcac ucccauacuc acggaguuau gaugacagca agaauaacaa ggugcuggua     1920

cuaacacaug aaaaccagaa uaaaggcaac agaacugcuu ucgaguaccu gaccuccuuu     1980

cccgaugggg aagacggaga gagguggcga acauucguug ccugggugca gggcaauaag     2040

gccuaucgga uggcuaaacg caacagacuc cugaggaaga auuacggugu cgacgaaucu     2100

aaaggguuua ucgaucggaa ccuuaaugac acccgauaua uaugcaaguu cuuuaaaaac     2160

uacguggagg agcacuugca acuggcagcc agagcggaug gcgacacggc uaggcgcugu     2220

gucgugguua auggacaguu aacugccuuc cuccgugcac gguggggccu gacaaaggug     2280

agaggggaua gcgacaggca ucacgcccuu gaugcugcag uagucgccgc gugcacccac     2340

ggaaugguga aggcucuggc cgacuacuca cgccgaaaag aaauuaguuu cuugcaggag     2400

gguuuuccug auccagaaac uggcgagauc cucaacccgg cagccuucga cagagcucgg     2460

cagcauuuuc ccgaaccuug gacacacuuc gcccaugagc ugaaggcaag gcuauuuacc     2520

gaugaccugg cugcccuccg cgaggauaug caacgucugg gguccuauac gaccgaagac     2580

cuuggaagau ugcggacacu guucguuagc agggcgccac agcgccgauc uggcggugcc     2640

gugcacaaag agacuaucua cgcacagccc gaaucauuaa agcagcaagg gggcgucauu     2700

gagaaaaucc uccugaccag ucuuaagcug caggacuuug auaagcuacu caauccugaa     2760

agcaacgacc acuucgugga gccacauaga aaugagcggc uguaugcugc cauuaggcag     2820

cgcuuggaac aauuuggagg cagagcugau aaagcauucg ggcccgacaa ccuguuucac     2880

aagccggaua aaaauaacca gccuacagga ccaguaguga gguccauaaa guuaguucgg     2940

ggcaaacaga cugguauccc cauucguggg ggacucgcca agaacgacuc uaugcugcgc     3000

gucgauaucu ucaccaaggc cggcaaauuu caucuugugc cuguguacgu ccaccaucga     3060

guuacagguc ugcccaauag agcuaucgug gcguucaagg acgaggauga auggaccuug     3120

auugacgagu cguucgcauu ucucuucagc guguauccaa acgauuacgu aaaagucacu     3180

cugaagaaag agcagcaauc cggcuauuac ucaggggccg acaggaguac gggagcuaug     3240

aaucuauggg cccacgaccg ggcagccucu gugggcaagg augggcugau acgcggaauc     3300

ggcguuaaga cagcucuuag cguggaaaaa uuuaacgucg acgugcucgg uagaauuuac     3360

cuggcgccuc ccgagaccag guccggguug gccuga                               3396


<210>  2827
<211>  3399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2827
auggcccagu acguguucgg ccuggacauc gggauugcua gcgucggaug ggcaguucuc       60

gaugcccaaa gaauagugga ccuuggugua aggugcuuug auaaggcgga gaccgcuaaa      120

gaaggcgacc cccugaacuu gacacggcgc caggcccgau uacugcguag aaggcuauau      180

cggcgcgcau ggagacucac ucagcuggcc aggcuuuuca agcgggaggg gcugaucgau      240

gacgcuaaau uguuugcaaa ggccccuucc uggacggaaa augcuuggga gcuccgccug      300

cgaggacugg aucaccucuu aaccccacug gaaugggcca gagugauuua ccaucuuugu      360

aaacacaggg gcuuccauug gacaucuaag gcggaggagg caaaggccga aucagacgcu      420

gagggugggc gggucaaaca gggauuggcc cacacuaagg cacugaugca agugaaaaac      480

uaucguagua ccgcugaaau gguucuagcc gaguuuccgg gcgcgcagcg caauaagaga      540

ggccaguacg auaaagcccu cagcagggug cugcuugggg aggaacuggc acuccucuuc      600

gcuacacagc ggcgacuggg aaacccccau gccuccgagc acuuugaaca auuaauccug      660

ggucacggcg accgcaaguc uggguuguuc uggcagcaga agccugcucu uucgggagcc      720

gaucuguuga aaaugcuagg caagugcacc uuugagaaaa cugaauauag agcaccaaag      780

gccucauuca gcgcugagag gcaugucugg cugacacggc ucaauaaccu gcgcauugug      840

guagacggua gagcgaggcc ccuuaaugau gcagagcguc aggccgcucu guugcucccu      900

uaccaaaccg aaacguuuaa auauaagacu cugaaagccg cauucguuaa ggugggguua      960

uggggagacg gcguccgguu uggcgggcug gccuacccaa gucaggcuca ggccgaggca     1020

gaaaaggcga aagaccccga ggaucaauuc cuagugaagc ucccugcuug gcacgaacug     1080

cgaaaagccu ucaaggcagc cggacaugag gcucuuuggc agcagaucuc caugccagcc     1140

uuggaggaag acccgacccu gcucgaucag auagcaacag ugcugucugu cuacaaagac     1200

gguacugagg uugugcaaca gcuuagacag cuggcuuugc ccgaaccuga ugccagcauc     1260

gcgguacucg agaagauuag guuugacaag uucuccucac ugaguuuaaa agcccugcgc     1320

cggaucgugc cacuaaugca gggcgggcuc agauaugaug aagcugucgc acaaauuccc     1380

gaguacggac accacagcca gaggaucgac ccuggcgcca cccgccaucu guaucuucca     1440

cccuuuuacg aggcucagcg aaaguaugcc gguaaagggg aucggauagg cucuaugcag     1500

uuccgugaag acgcagaugu gccgagaaac ccuguugugu ugagggcgcu gaaucaagcc     1560

cgcaaggucg ugaacgcucu cauucgggag uacggauccc ccguagccgu uaauaucgaa     1620

auggcaagag accugucaag gccauuagac gagcgaaaca aagugaagcg cgcucaggag     1680

gaauuucggg auagaaauga caaggccagg agcgaguucg aacgugauuu uggcuauaaa     1740

ccuaaggggg cagucuucga gaaauggaug cuguaccgcg aacagcuugg acagugugcc     1800

uacagucaac agcccuugga cauucagcgg gugcucgaug acccaaacua ugcucaaguc     1860

gaucacgcgc ugcccuacuc uagauccuau gacgauagca agaauaacaa agugcuaguu     1920

cugacaaggg agaaccagaa uaaggguaac cgaaccccuu uugaguaccu cacgucguuc     1980

ccaggcgggg aagacggaga gcgguggcgc gccuuugugg cauggguaca gggcaauaag     2040

gcuuauagaa uggccaaaag gaaccggcug cuucgaaaga auuacgguac ugaugaaucu     2100

agaggguuca ucgaccgcaa cuugaaugau acaagguaua ucugcaaauu uuucaagaac     2160

uacgucgagg aacaucugca guuagccgcu cguaccgacg gcgacacugc acggagaugu     2220

gugguuguga auggacaacu cacagccuuc cugagggcgc gcuggggccu ugcuaaaguc     2280

cgaggggauu ccgacagaca ccaugcccug gaugcagccg ugguagcugc augcacccac     2340

ggaaugguga aggccuuggc ggacuacuca cggaggaaag agauugcuuu ucuccaggag     2400

gguuucccgg aucccgaaac gggcgagaua cugaaccuag ccgcauuuga ccgcgcccgu     2460

gaacacuucc cugagccaug gccccauuuu gcucacgaac ugaaggccag acucuucauc     2520

gaugaccugg uugcacuucg ggaggauaug cagcauuugg ggaguuaugc ucaggacgau     2580

cuggacaggu uacgcacccu cuuugucagc cgagccccuc aaagacggaa uggaggcgcg     2640

gugcacaagg guacaauuua cgcccagcca gagaaccuga aacagcaggg guccacuauc     2700

gaaaagauac uucugaccgg ccuaaaacuc caagauuucg acaagcuguu gaaucccgag     2760

ucuagcgacc acuuugugga accgcauagg aacgagcgcc uguaugcagc uauuagaagg     2820

cgguuagagg aguucggagg caaagccgau aaggcuuuug ggccugacaa gccauucuac     2880

aaacccgaua agaauggaca gccuacaggc cccaucgucc guucaaucaa acucguucgc     2940

gguaagcaga cugggauucc aauccgagga ggccuggcca aaaacgacag uaugcuuaga     3000

guggaugugu uuaccaaggc agguaaguuc caccugguac cugucuaugu gcaucacagg     3060

guuacaggcu ugcccgaccg ggccauugug gcuuucaaag augaagaaga guggacccuc     3120

auagacgaau ccuacgcguu ucuguucagc gucuauccaa acgauuuugu guguguucaa     3180

cagcgcggga agccgcuaau cgagggauac uacgcaggcc uggacagagc caaugggaac     3240

auuaaugugu gggcucauga uagggccgca ucuguaggaa aagacggccu uauccggggu     3300

aucgggguca agacugcccu cucacuggag aaauugaacg uggacgugcu gggacgaauu     3360

uauccugcuc ccccagaaac gagacgcggc uuagcguga                            3399


<210>  2828
<211>  3399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2828
augaagaaaa uccugggcgu ggaccucggg auuaccagcu ucggauacgc cauacuucag       60

gagacaggua aggaucugua uagaugcuug gacaacuccg ucguuaugag gaauaacccc      120

uacgaugaaa aaucuggcga gucaagucaa agcauccggu ccacucagaa gucuaugcgc      180

cgauuaauug aaaaacguaa gaagagaauc aggugugugg cucagacgau ggagcgguau      240

gggauucugg acuacucgga aaccaugaaa aucaaugauc cuaagaacaa uccaauuaaa      300

aaccgcuggc agcuaagagc aguagacgcc uggaagaggc cgcucucacc ccaagagcug      360

uuugcgaucu ucgcucacau ggccaaacau cggggauaua agagcauugc aacagaggau      420

cuuaucuacg aacuggaguu ggaacucggc cugaaugacc cugagaagga gagugaaaag      480

aaggccgaug agcgccgaca gguguauaac gcucugagac accucgaaga guuaaggaaa      540

aaguacggug gggaaacuau ugcacagacc auccaucggg ccgucgaggc uggagaccug      600

cguuccuacc gcaaucacga ugacuaugag aaaaugauaa gaagggaaga uauugaggaa      660

gagaucgaaa aggugcuuuu gcggcaggcc gagcugggcg cgcuaggccu cccagaggaa      720

caaguuucug agcugauuga cgaacuuaaa gcaugcauca cagaccagga gaugcccacc      780

aucgaugaaa gccuguuugg gaaguguacu uucuacaagg acgaguuggc cgcuccugcc      840

uauuccuacc ucuaugaucu guaccgauua uauaaaaagc uggcagaccu caacauugau      900

ggauacgagg ugacacagga agaccgcgag aaagucauag aaugggugga gaagaaaauc      960

gcucagggua agaaucuuaa gaaaauuacc cauaaggauc ugagaaaaau cuugggccua     1020

gccccagagc aaaagauauu ugggguagaa gacgagagga uuguuaaagg aaagaaggaa     1080

ccccggacgu ucgugccauu uuucuuucug gcggauaucg ccaaauucaa ggagcucuuu     1140

gcaucaaucc agaaacaccc ugacgcucug cagauuuucc gcgaacuugc cgagauccug     1200

caaagaagua agacuccgca ggaggcuuug gauaggcucc gugcccugau ggcaggcaaa     1260

gguauugaca ccgacgaucg ggaauuacug gagcuauuua agaacaagcg aagcgggaca     1320

agagaacucu cucacaggua cauacuggag gcccuucccu uguuccugga aggauaugac     1380

gagaaagagg uccagcgcau ccucggcuuc gaugaccggg aagauuacuc cagauauccu     1440

aagucacuga ggcaucuuca ucugcgcgag ggcaauuugu uugaaaaaga ggaaaaccca     1500

auuaauaacc acgcugugaa aagccucgcg aguugggcac ugggguuaau cgccgaccug     1560

ucuuggcgau acggacccuu cgaugagauc auucuagaga cuacccggga cgcucucccu     1620

gagaaaauac guaaggaaau cgauaaagcc augagagaaa gggagaaggc acuggacaag     1680

auuaucggua aauauaagaa agaguuucca uccauugaua agcgccuugc ccggaaaauc     1740

caguuguggg aaagacaaaa gggccuggac cucuacagcg ggaaggugau aaaccugucg     1800

caguuacugg auggaucugc ugacaucgag cacauugucc cccagucccu uggcgguuug     1860

ucaacagacu auaauaccau cguuacgcuc aaaaguguga acgccgcaaa ggggaauagg     1920

cugccgggcg auuggcuagc gggaaacccu gacuaccgag aacgcauugg caugcugagc     1980

gagaaagggc ucaucgauug gaagaaacgg aagaaucugc uugcucaguc cuuggacgaa     2040

auauacacug agaacacaca cucuaaggga auuagagcca ccagcuaucu ggaggcauua     2100

guagcccaag ugcucaaaag guacuauccc uucccagauc cugaacugcg uaagaauggu     2160

aucggcgucc gcaugauucc cgggaaagug acuucaaaga cacggagucu ucugggaauc     2220

aaauccaaga gcagagagac caacuuucau cacgcugaag acgccuugau acucucuacg     2280

cugaccaggg gcuggcagaa ucgacuacau cggaugcugc gcgauaacua cgguaaguca     2340

gaggcagaac ucaaagagcu guggaagaaa uauaugccac acaucgaggg gcuuacauug     2400

gcugacuaca uugaugaagc cuucagaagg uuuaugagua agggcgagga aagccuguuc     2460

uaucgggaca uguuugauac uauccgaucc auuucuuacu ggguugacaa aaagcccuua     2520

ucggcgagcu cccauaaaga gaccguguac ucaaguagac acgaaguccc uacacuccgc     2580

aagaauaucc uggaggccuu cgauucucuu aacgugauaa aggacaggca caaacugacu     2640

accgaggaau uuaugaagcg uuaugacaaa gagauucggc agaagcuaug gcuccauaga     2700

aucggaaaua caaacgauga aagcuacagg gcuguagagg agcgcgcaac ccagaucgcc     2760

caaauucuga cucgauauca guugauggac gcucagaaug auaaagaaau cgacgagaag     2820

uuccagcaag cccugaagga auuaauuacg uccccaauag agguuacagg caaacuccug     2880

agaaagaugc gguuugugua cgauaaacuu aacgcaaugc agaucgacag ggggcugguc     2940

gaaaccgaua agaacauguu gggaauacac aucucaaaag guccgaauga gaagcucauu     3000

uuccgccgua uggacgugaa caaugcgcau gagcugcaga aggaaagaag cggcauccua     3060

ugcuaucuga acgagaugcu uuucauauuu aauaaaaagg ggcucaucca cuacggaugu     3120

cugcggucuu auuuggaaaa aggccaaggu uccaaguaca uugcccuguu caaccccagg     3180

uuuccugcua auccaaaagc ccagcccagu aaguucacua gcgauucuaa gaucaaacag     3240

gucgggauug gcuccgcaac cggaaucaua aaggcucacu uagaccucga uggccaugug     3300

cgcucauacg agguuuuugg gacacugccu gaaggaagua uugagugguu caaagaggaa     3360

agcggcuaug gucgagugga ggacgaccca caccauuga                            3399


<210>  2829
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2829
auggaccuga acuacagaau cggccucgau auugggauag ccagcguggg augggcuguc       60

cuugagaaua acuccaagga cgaacccguu aggaucgugg aucugggugu acgguuguuc      120

gaggcagccg aagugccuaa aaccggcgcg gcuuuagccg agccacgccg agaagcacgu      180

acaacuagaa ggcggcugcg cagaaggaag caccggcuag agcgcaugaa auggcuccug      240

caggaguaug ggcuuauuca aacggaagag uuuaugaagc gauaccauuc ugccggacug      300

ccggacgucu auagauugag guacgaaggc cucgagcgga aacugaagga ugaggaacug      360

gcucagaucc ucauucacau cgguaagcau cguggguucc gcucaaccag aaaagcagag      420

uuaaaggaca aaaaugauaa ggaaacagga gccgugcuga gugcuacuag ggagaacgaa      480

aaacuuaugg aggagcacgg cuaucggacc gccggcgaaa ugauauacaa ggacgauaag      540

uuucgaacag ggugcaccug gaaagaggaa ggauaucgcu ugacucccag aaauaaggag      600

ggugacuaca aaaacacaau gcugagggcg cuacucgaag augagguuaa ggugaucuuc      660

gacugucagc ggaaauuugg caaucagaag gcaagcgagg aacuggagaa agccuaccuu      720

gcuauuauga ccucccaacg cucuuucgau auggggccug gaaagcagcc agacggcucg      780

gccucacccu augcaaugga agguuuuggg gacagagucg gacugugcgc uuuggagccu      840

agggaaaagc aggagccacg ugcccccaaa gcggccuaca cgagcgaacu cuucguggca      900

cugcagaaga ucaaccauuu acggauuacu gaaaagaaug gcaagggccg agggcugacc      960

agugaggaaa gagagaggcu cuccgaucuu cugcacaaaa agaaggaguu gacauaugga     1020

gcuguacgca aagaacuaca acuggaccuc cgguacagau uuaacacucu gaauuauucu     1080

agcaagaaaa ugaccgagga agagaagauc aaagaugccg aaaaggcuaa guucguuucc     1140

auggugucac acuacgagua uaggaaacgc augaguggua ugcuugagca gcugagcccu     1200

gaggagcagg ccgacuugau ggaugaaguc ggcaagauac ucacacugua caaaaacgac     1260

gauacccgau cucgggaguu ucagaaguua gggcuggacu ccgaaauuac ggaggagcua     1320

cucgaacuga auccacgugg auuccaacau gugucacuua aagcaaugaa gaaguugaug     1380

ccguaccuga aagagggccu cacuuaugau aaggccugug cugaagcggg uuacgacuuu     1440

caggggagcg ugcagaaaga gagaaguagg cugcuuaagg gcaaagaaau cacagauauu     1500

aucaacgaca ucaauaaccc cgucguuaag cgcucugugu cccaaaccgu aaaagugauu     1560

aacgcaauaa uccagaagua uggaagcccu caggccguca auauugagcu ggcucgggag     1620

augucgagaa acuacgacga aaggcgaaag auggagaaac agaugaagga ucgccaaucu     1680

gaaaaugagc gggccaaaaa ggagauccag gaauugggca acgcauuccc aacugggcag     1740

gacauacuca aauauagacu guggcacgau caggacggaa uuugcaugua cuccgccaag     1800

aggauccccu uagcugagcu guucacaggu gccuaugaua ucgaccauau ucuaccuuac     1860

ucaaucaccu uugaugacag uuaccguaau aaggugcucg uuacgagcga agagaaccgc     1920

caaaaaggca aucggauucc auaugaauac uucggguccg auucugagag auggagcaag     1980

uuugagaccc uggugaacuc aaggauacga gacuaucgga aacagcagaa ucuuuugaag     2040

cagaguuuca cagaagagga acgcaaagag uuuaagcaaa gaaaccugaa ugauacuaag     2100

uacaucacca gggucauuua uaacaugauc cgggaaaauc ucgcacuggc gcccuacaac     2160

uccccgggca aaaagaaaca gguguucgcu guaaauggag guguuacagc cuauuuacga     2220

aagagauggg ggaugagcgc aaaagaccgc ucuaucgaua cucaccacgc cagggacgcu     2280

guggucauug ccuguugcac cgacggcaug auacagaaga ucucacguaa cauucaggga     2340

cgggagcugc uuuacgcaag aggcuuuaca gcuguggaug ucgagaccgg ggaaaaguuc     2400

acuagggacg auuuuacgcg cgaccaaugg gaugagaucu ucggagugca gguuccuaaa     2460

cccugggaau acuuuaacga ggaauuggac auucgacucg gugagaaucc aguggaguuc     2520

aucgaaacac auagugaugu aagcagaaag cuggacuauc cugccugguu uuacgagaac     2580

aauauaaucc ggcccgucuu cguguccagg augccaaacc gcaaaguuac cggcgcggcc     2640

cacgcugaua cuauucguuc ucccagacau uaucaggaaa ccgggucggu gcuaacaaag     2700

accgcacuga ucgaccucaa acugaauaag aaaacuggag agauugagaa cuacuauaag     2760

ccugaaagcg auacacuuuu guacaaugcc cugaaggagc gguuagaacu cuauggcggu     2820

gacgggaaaa aggcuuucga ggaaccauuu uacaaaccga aggccgaugg cuccagggga     2880

aaccuggucc gcaaagugaa gacguacgac aaacuuaccc ugggcguaua uguuaauuca     2940

caacacggga ucgcagcgaa cgccaaugga aguauggugc gagucgacgu guucagagug     3000

ucuggcaaau acuauuuugu ccccguuuac auagcugaug uggugcggaa gagguugccu     3060

augaaagccg uaguccaggg uaagcaguau auugacugga aagagaugga ggauaaggac     3120

uucaucuuua gccucuacac ucgcgaucug auuuccuuca agagcaaaaa cgggaagaaa     3180

gugacaugug uugacggaac cucaaaggca gugaaugaag agaucgucua uuacuacggc     3240

gcuaacauau cuacagccuc cuuuaguggu aaaagccaua auggcucuuu cgaauuuacu     3300

ucccuaggga uccagggacu ggaguaucuc aagaaguacc aaguggaugu ucugggcaac     3360

gugucagaag uaaaaagaga gaagaggaug ggguuccggu ga                        3402


<210>  2830
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2830
augggcgggu ucgaggugga cagaaagauc agccugggac ucgauauugg ugucuccucu       60

guuggcuacu cagugauaga cguagauacc gggaaagugc uugaacuggg aaguagguug      120

aucaacggca agauugguac agagaaucag gcccggcgcg aaaugcgagg gacucguaga      180

uuaaucaaca ggaaaaagca acggcgcaaa gacacgguca agcuguuuaa ugaguucaag      240

cuaauugaua gcaaaaagga cgaacacuau uuugaucauu ucgcuaacaa uuccgacccc      300

uacgagcuca gagugaaagg acugucugag aaacuuucgc ugucagaaau cgcagauggc      360

uuguaucaca uaguuaagag gcggggcauc agcuacgacc ucaaggaugc cgacgaggaa      420

agugaggggu ccgaguauga aaacggacug aagauuaaua acucugagcu gaaaaaucag      480

accccugcgc agauccagcu caagcgcuua aacgaauacg gugcuauucg aggcaaagug      540

gucaucgaug aggaaaagau acugcuuaau guguuuccaa caaaagccua uuuggacgag      600

gcaaagagaa uuaucgagaa acaaagggag uucuacccgg ggauucugac ugaggaauuu      660

gaggaaaagu acugccagau ccuaacccgg aagcgugacu auuucguagg acccggcagc      720

gagaaauccc gcacagauua cgguaucuau aaggaggacg ggagaacccu caacaaucug      780

uuugaagaac uuauuggaca ugauaaaaua uacccugagg aacugagggc cucaggcgcu      840

aguuucacug cacaguuguu uaacguucuc aaugaccuga acaacuuaca caucagcucu      900

uaugaagauc agaagcugac aaccgagaau aaagagcaaa uuaucuccga acucaaguca      960

acgacuagca acgugaauau gaugaaacuu auacagaaga uugccggcug ugaggaaagu     1020

gacaucucug gguaccggac cgauaagaac uauaaaccag agaucuccag caugcgaauu     1080

uaccgcaagg uccauaaaaa gcuguuggag ucggaaaucg acauuacaaa uuggcccgau     1140

gacuucauaa acaagcuauc uuuuauccug acucucaaua ccgagaacgg agaaauuaga     1200

aaucagcuga aaaacaggcu ucagccugag uacgauuucc ugacagacga auugauccaa     1260

accaucauug acaauaagga guccuucgau auaaaaucaa acaauaagug gcaccgguuu     1320

aguaucaaaa cgauggagcu ccugauucca gaaaugauca agcgccccgu ggagcagaug     1380

acuuuaauua acgaaauggg ucugaucaag aauagcggca aaaaguucuc caacuauaaa     1440

uaccuaccuu auaagaaaau agcugaggac aucuacaacc caguggccuc uaagagcguc     1500

agagaagcgc ucaaaauugu uaaugcaauc cugaaggagu augggcacau ugaguacauc     1560

gugguagaaa ugccgaggga uaagaacgag gacgaagcca aagaucagau agagaaguuu     1620

caaaaagcua auggacgucu uaaggacgcc gcauuggaag aguucacaga ggcuauuggc     1680

gauaaaucaa ccguggacaa gaagcugggu guccaucgga guaaacuccu guuuaagauc     1740

cgacuuuggu aucagcagag agggauggau cuguacgaag gcaaacccau uuccgccgug     1800

gacuugcuca acaauccuga uagcuuccag aucgaccaca uaaucccaca aucuguuuca     1860

uuugaugaca guuucaacaa uaagacucug ugcaacagcg gauuaaauca gauuaaagac     1920

aagaagacac ccuuugagua caugaacgaa ggcuaugggc agggauucga uuccaugaaa     1980

gcgaugguga ugaagaaucc uaggaucgac cauaacaaac gcaagaauua ccuguuuucu     2040

gagaacgucu cggauauuga gacccggaaa agauucauca gcaggaaucu aguggacacg     2100

cgcuaugccu cccgaguagu ucucaacaac cugcaggaau uuuucaagga gaaggaauca     2160

gauaccaaaa uaaguauuau ccgggguaag uucacaucua aucuucguaa acacuggaag     2220

aucaacaaaa auagagacac uuaccaccau cacgcaauug augcuaccau cauugccgcu     2280

acaccauuuu ugaacauaug gaagaaaggc agcuccaucu uccccucaaa uguggagagc     2340

gaagacuuuu ucgauauuaa gacugggaag aucauuggaa acgacgaguu uaauaaauau     2400

gccuacgaug agccguauaa cggcuucuac aaugaacuga ggaacgcaac cgaccgcauc     2460

aaguuuucuc aucaaauaga uaaaaagaug aaucggaaag ucuccgacag uacaaucuac     2520

agcaccagag uggccaaguc uccuaagaac aaaaaggagu ccgaauauau ugucgcuaaa     2580

aucaagaaua uuuacgacgc ggaugaguau gcaaaauuca agaggaucua cgacaaggau     2640

aaaacuaagu uucuccugaa caaauaugac ccccgaucau ucaaucacuu agaaaagaua     2700

augaacacgu acccagauag uauugagcgc acaaugagca acaauaaagu gaagguugug     2760

cggaucucgc cuuuugagcu guauagacag gaccaugguc ccguaaagaa auacuccaag     2820

aaaaacaaug ggccagaaau caagcagcuu aaauacuugg auaagaaggu cggcucuagc     2880

auugacguga cccccgauaa cgccaaaaau aagcacguua uacucaaauc acugaagccu     2940

uggaggacug acaucuaucu aaaccacgag accggagaau acgagauuau gggcaucaaa     3000

uauagugauc ugaaguucaa uaaaggggaa ggauacggua uuaagaaggc uaaauaucuc     3060

gagaucaagc aucgugagaa aguguccgac gacucugaau uuauguucac acuguacaag     3120

aaaaaccgca uaaaggucga gaauauggaa accgaugaga gcguggagau gcuuuucugg     3180

ucccggacua uggacucaca gaagggcuau gccgaauuga aaccaaucua caaguacaaa     3240

acagaggaug acaacuggcc gauuuauggg aaugcaaaga aacaaaucau uaagaagcug     3300

auccccaaaa acuguaagau auggaaagua aauacggaug uguuaggaaa cccuuuuuac     3360

guugaaaagg agggcgaaua uccaaaagac guccucgauu ga                        3402


<210>  2831
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2831
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaaaauu ccgggaaaau cgugcacgcu ucuucaagaa uuuuccccgc agccaccgcg      120

gauaacaaug uagaaaggcg gaagaaccgc caggcucgac guuugcauag aaggaaaaag      180

caccggggag cccgcuuaaa agaccuguuu gaguacuaug gccuacucac agauuucagu      240

aaggugagca ucaaucugaa cccuuacaga cuuagggucg acggucugga ucaacaguug      300

acuaaugaag aacucuuuau ugcacugaag aacaucguga aacggcgcgg gauauccuau      360

cuggacgaug ccucugaaga cggaggcacg guuucgucag auuacggcaa ggcuguggag      420

gagaaucgaa aacucuuagc agaacagacc ccagggcaga uccaacugga gagauucgaa      480

aaguauggac agcuuagggg ugacuuuaca gucguggaga acagcgagaa augccgguug      540

auuaauguau ucaguacuuc cgccuacaag aaggaagcug agcguauccu gcgcaaacag      600

caggaauuua acaaucaaau uaccgaugag uucaucgaag acuaucuaaa gauacucaca      660

ggcaaaagaa aguacuacca ugggccggga aacgagaaau cuaggaccga uuauggccgg      720

uuucgaacug acggugccac acuggacaau auuuucggga uccuuauugg aaaguguacc      780

uuuuacccca acgaguaucg cgcgagcaaa gcauccuaca cggcccagga guucaaccug      840

uugaaugauc ucaacaaucu gacuguuccu accgagacaa agaaguuauc agaagaacag      900

aaaaagacua ucaucgaaua ugcuaaaagu gccaagaccc ugggcgcaag cacacuccuu      960

aaauacauug cuaagaugau agacgccucu guggaucaga ucagaggcua uagggucgac     1020

gugaacaaua agccagagau gcacaccuuu gagguguacc ggaaaaugca aucccuggaa     1080

acgauuucag ucggggaguu gagccgcaac auccuagaug aacuggcgca uauacucacu     1140

cugaauacag agagagaagg aauugaggag gccaucaaca ccaagcuaag ggacaguuuc     1200

ucucaggauc agguucugga auuggugcaa uuccguaaaa auaacuccag ccucuuuucg     1260

aaggguuggc acaauuucuc ucugaaauua augauggagc ugauccccga acuauacgag     1320

acuuccgagg aacagaugac aauucucacc cggcugggca agcagaaguc aaaagagacg     1380

aguaagcgaa ccaaauauau cgacgaaaag gagguaacag aagagauuua caacccugug     1440

gucgcaaaaa gcgugagaca ggcuauaaag aucauuaaug aggccacuaa gaaacacggg     1500

aucuuugaua acaucguuau ugaaauggcu agggagaaua acgaagagga cgccaagaaa     1560

gauuauauaa agcgccaaaa agcaaaccag gacgaaaagu acgccgcuau ggagaaagcg     1620

gcauuccagu auaauggaaa gaaggagcuu ccagacaaca ucuuucaugg ccacaaagaa     1680

uuggccacca agauucggcu guggcaucag caaggugaga aaugccucua cacagggaag     1740

uccaucccca uuucugaucu gauccacaau caguauaaau acgaaauaga ccacauccuu     1800

ccucugagcu ugucauucga ugacagucuc uccaacaagg ugcuggucuu agcuacugcc     1860

aaucaggaga agggccagag aaccccauuu caagcacugg auagcaugga cgaugccugg     1920

ucuuacaggg aguucaaauc auaugugaag gagaguaaac uacucggaaa caagaaaaag     1980

gaguaccugc uuacagaaga ggacauuagc aagaucgaag uaaaacagaa guuuauugag     2040

cgcaauuugg uugauacccg auauuccucu cggguggucc ugaacgcucu ccaggacuuc     2100

uacaaaaauc auaacuuuga uacuacgauc ucgguggucc guggccaguu cacaagccaa     2160

cugagaagga aguggggguu agagaaaucc cgcgaaaccu aucaccauca cgccguggac     2220

gcacugauaa uugcggcuuc aagucagcuu cgguugugga agaagcagaa uaacccgcuc     2280

aucgccuaca aagagggaca auuuguugau ucucagacug gugaaauuau cagccugacc     2340

gacgacgagu auaaggaacu uguguucaaa gcacccuacg aucauuucgu agacacacug     2400

uccucaaaga ccuuugagga uagcauacuc uucucuuacc aggucgacuc caaauuuaau     2460

agaaagauca gugaugccac uauuuaugcu acaaggaaag ccaagcugga caaggagaaa     2520

aaggaauaca cguauacccu uggcaaaauc aaggauauuu acagcuuggg gacuaaaaca     2580

ccuucuaaga ccggauucua uaaguuucug gacuuauaca acaaagauaa gucccaguuc     2640

cucauguuuc aaaaagaccg aaagacaugg gaugagguga ucgaaaagau aauggagcag     2700

uaucgcccau ucaaggaaua cgacaaggca ggcaaacugg uugauuuuaa ucccuucgag     2760

aaguaccggc aggagaacgg uccuauuaga aaauauucaa agaaagggaa cggcccagaa     2820

aucaagaguc uuaaguacua ugacauccug uugggaaaac acaagaauau uacucccgag     2880

ggcagcagga acaccguggc ucuccugucg cuaaauccgu ggcguacgga cgucuacuau     2940

aacauggaaa ccaaaaagua cgaguuucug gggcucaaau augccgaucu gccuuucgaa     3000

gagggaggug cguacggcau cuccacagag acuuacaaug aacuucgcga gaaggaaggg     3060

auuggaaaga acucugaguu uaaauucacc uuguauaaga augaccugau auuaaucaaa     3120

gauacagaga cuaacuguca gcaauucuuu cgguucugga gcagaaccgg caaggacaau     3180

cccaaaucau uugaaaagca caaaauugag cucaagccau acgaaaaggc caaauucgag     3240

aagggugagg aacuggaggu gcuugggaaa guaccuccca guuccaacca guuucagaag     3300

aauaugcaga ucgaaaaccu gucuauuuau aaagugaaga cggauguucu aggcaauaag     3360

cauuucauca aaaaggaggg agacaaacca aagcucaaau uuuga                     3405


<210>  2832
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2832
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaaaauu ccgggaaaau cauucacgcu aacucuagau uguuccccgc agccaccgcg      120

gauaauaacg uggaaaggcg gaagaaucgc caggcucgac guuuaaacag aaggaaaaag      180

caucggggag uacgcuuuca agaccuguuc gaggauuacg gccuacucac agacuuuuca      240

aaagugagua ucaaucugaa cccuuauaga cuuagggucg auggucugaa ucagcaguug      300

acuaacgaag aacucuucau ugcccugaag aauaucguga agcggcgcgg gauaagcuac      360

cuggacgaug cauccgaaga cggaggcgcc guuucuucgg auuauggcaa agcuguggag      420

gagaaccgaa agcucuuagc agaacagacg ccagggcaaa uccagcugga gagauuugaa      480

aaauacggac aggucagggg ugacuucacc gugguagaga auggcgagaa acuucgguug      540

auuaacguuu ucucaacaag cgccuauaaa aaggaagcug agcguauccu gcgcaagcag      600

caagaauuua auaaccagau uacugaugag uuuaucgaag acuaccuaaa aauacucacc      660

gggaagagaa aauauuacca cggaccgggc aacgagaaga guaggacaga uuacggucgg      720

uuccgaaccg acgggacuac acuggacaau auuuuuggaa uccuuauugg caaaugcacc      780

uucuaucccg aggaauaccg cgccuccaag gcgucuuaua cggcacagga guuuaaccug      840

uugaaugauc ucaacaaucu gacugugccu accgaaacaa agaaguuaag cgaggaacag      900

aagaaaguca ucguggagua cgccaaggag gcuaaaacuc ugggcgccuc cacccuccuu      960

aaguauaucg caaaaaugau ugacgcuuca guggaucaaa uaagagggua cagggucgac     1020

guuaacaaua agccagaaau gcauacauuc gagguguauc ggaagaugca gagucuggaa     1080

accaucagcg uaggagaguu gucucgcaac gugcuagaug aacuggccca cauucucacg     1140

cugaauacug agagagaggg uauggaagag gcgaucaaca caaaacuuaa ggacuccuuc     1200

ucacaggauc aaguccugga auuggugcag uuuaggaaaa auaacagcag ucucuucucu     1260

aagggcuggc auaauuuuuc ccugaaauua augauggagc ugauacccga gcuauacgaa     1320

accagcgagg aacagaugac uauucucaca cgucugggga agcagaaguc gaaagagacc     1380

ucuaagcgga cgaaauacau cgacgaaaag gagcuuaccg aggaaaucua uaacccuguu     1440

guggccaaau ccguccgaca agcaauuaag aucauuaaug aggcuacaaa gaaacacgga     1500

auauucgaua acaucgugau ugaaauggcc agagagaaca augaagagga cgcuaagaaa     1560

gauuacauca agaggcagaa agccaaccag gacgagaaga augcagccau ggaaaaagcu     1620

gcguuucagu auaacggcaa gaaggaguug ccagacaaua ucuuccacgg ucauaaagaa     1680

cuggcaacua agauucgccu cuggcaccaa cagggggaga aaugucugua caccggcaag     1740

acaauaccca ucucagaucu uauucauaac caguauaaau acgaaaucga ccacauucug     1800

ccuuugaguc ucagcuuuga ugacucccug ucuaauaagg uauuaguucu ggccacugcu     1860

aaccaggaga agggacaacg gaccccauuc caggcccuag auagcaugga cgaugcaugg     1920

ucauauagag aguuuaaaag uuacgugaag gaauccaaac uccugggcaa uaagaaaaag     1980

gaguaccuuu ugacagaaga ggacaucagc aagauagagg ucaaacagaa guucaucgaa     2040

aggaaccuga uugauacccg cuauucuuca cgaguggucc ucaaugcccu gcaggacuuu     2100

uacaaagcuc acaaguucga uacuacgauc agugugguuc gggggcaauu uacaagccag     2160

uuacguagaa aauggggacu ggagaagucc agggaaaccu aucaucacca ugccguggac     2220

gcacuuauua ucgcggcuuc uucgcaguug cgccucugga agaaacaaaa caauagccug     2280

auauccuaca aggaggguca guucguagau ucagaaacug gcgagauuau cagucuaacc     2340

gacgacgagu auaaagaacu ggucuucaag gccccguacg aucacuuugu ggacacacuc     2400

cggucuaaaa aguucgagga uagcauucug uuuuccuauc agguugacuc aaaauucaac     2460

agaaagauca gcgaugcaac cauauacgcc acuaggaagg cuaaacuuga caaggaaaag     2520

aaggaguaca cauauacguu ggggaaaauc aaggauauuu acucucuggg aaccaaggcc     2580

cccuccaaaa cuggcuuuua uaaguucuua gaccucuaca auaaagauaa gagucaguuu     2640

cugauguucc aaaaagaccg aaagacaugg gaugaaguga ucgagaagau uauggagcag     2700

uaucgcccuu uuaaagaaua cgacaagacc gguaaacuug ucgauuucaa cccauuugag     2760

aaguaucggc aggaaaacgg gcccaucaga aaauacagca agaagggcaa uggaccugag     2820

auaaaaucuc ugaaguacua ugacauuuug cucggcaaac auaagaacau cacaccagaa     2880

ggguccagga auacuguggc acugcuauca cugaaccccu ggcguaccga cguauacuau     2940

aauauggaga cgaaaaagua cgaguuccuc ggacugaagu augcugaucu uccguuugaa     3000

gaggguggcg ccuacgggau caguaccgaa acauauaacg aguugcgcga gaaagaagga     3060

auuggcaaga auagcgaguu caaauucacu cuguacaaga acgacuuaau ccucauuaaa     3120

gauaccgaaa caaauugcca gcaaauauuu cgguucuggu cgagaacugg uaaggacaac     3180

ccuaaauccu uugagaagca caagaucgaa cugaaacccu acgagaaggc gagguucgag     3240

aaaggggagg agcuuaaggu gcugggcaaa guuccaccuu cuagcaauca gcuacagaag     3300

aacaugcaga uugaaaaucu cucaaucuau aaggucaaaa ccgaugugcu gggaaacaag     3360

cacuuuauua aaaaggaggg cgacgaaccc aaauugaagu ucuga                     3405


<210>  2833
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2833
augaacggcc uggugcucgg gcuugacaua ggaauugcca gcgucggugu uggcauacug       60

gagaaaaauu ccgggaaaau cauucacgaa aacucuagau uguuccccgc ugcaaccgcc      120

gauaauaacg uggagaggcg gaagaaucgc caggcgcgac guuuaaacag aaggaaaaag      180

caucggggag uacgcuuuca agaccuguuc gaagauuacg gccuacucac agacuuuuca      240

aaagugagua ucaaucugaa cccuuauaga cuuagggucg auggucugaa ucagcaguug      300

acuaacgagg aacucuucau ugcucugaag aauaucguga agcggcgcgg gauaagcuac      360

cuggacgaug ccuccgagga cggaggcacg guuccaucug auuauggcaa agcagugaag      420

gagaaccgaa aacucuuagc cgaaaagacc ccggggcaga uccaacugga gagauuugaa      480

aaauacggac agcuuagggg ugacuucaca gucgaggaga auggcgaaaa gugccgguug      540

auuaacgugu uuucgacuuc agcuuauaag aaagaggcag aacguauccu gcgcaagcag      600

caggaguuca auaaccaaau uaccaacgaa uuuaucgagg auuaccuaaa aauacucaca      660

gggaagagaa aauauuacca cggacccggc aaugaaaaga gcaggaccga cuacggucgg      720

uuccgaacug augggacaac ccuggacaac auuuuuggaa uccuuauugg caaauguacg      780

uucuauccug aagaguaccg cguaaguaag gccuccuaua cugcucagga auuuaauauc      840

cugaacgacu ugaauaaccu caccguucca acagagacua agaaacuguc ugaggagcag      900

aagaaagaua ucguggagua cgccaaggaa accaaaacau uaggcgcgag cacccugcuc      960

aaguauauug caaagaugau agacgccucc gucgaucaga ucagagggua caggguggac     1020

gugaauaaca aacccgagau gcauacguuc gaagucuauc ggaagaugca aucacuugag     1080

acuauuagug uuggagaacu gagccgcaau guguuggaug agcuagcuca cauccugaca     1140

cucaacaccg agagagaagg uauagaggaa gccauuaaua cuaaacugau gggcguauuc     1200

ucucaggacc aggugcuuga gcugguccaa uuuaggaaga acaauuccuc auuguucagc     1260

aaaggguggc auaacuuuag ucucaagcug augauggagu uaaucccuga acuguacgag     1320

acaucugagg aacagaugac cauccuaacg cgucucggaa agcagaaauc caaggaaacc     1380

agcaaacgga caaaguacau ugaugagaaa gaggugacug aagagaucua uaauccaguu     1440

guggcaaagu cgguccgaca ggcuauuaag auaaucaacg aagccaccaa aaaguacggc     1500

auuuucgaca acaucgugau cgagauggcg agagaaaaua acgaggagga ugccaaaaag     1560

gacuauauua aaaggcaaaa ggcaaaucag gaugaaaaga acgcugccau ggagaaggcu     1620

gccuuucagu acaaugguaa gaaagaacug cccgacucua uauuccacgg gcacaaggag     1680

cuugcaacaa aaauccgcuu guggcaucag caaggcgaaa agugccugua uacuggcaaa     1740

aacauuccua ucuccgaccu cauucacaau caguacaagu augagaucga ucauauacug     1800

ccacuuucac ugaguuuuga cgauagcuug uccaacaagg uacucguucu ggccaccgcu     1860

aaucaggaga aaggacagcg gacaccguuc caagcguuag acucuaugga ugacgcaugg     1920

agcuacagag aauuuaaguc auacgugaaa gauaguaagc ugcuauccaa caaaaagaag     1980

gacuaucucc ugaccgagga agauaucagc aaaauugagg ucaagcagaa auucaucgag     2040

aggaaucuug uggacacucg cuacucuuca cgagucgugu ugaacgcccu gcaggauuuu     2100

uauaaggcuc acaaauucga cacgacaauu aguguugugc gggggcaguu uaccagccaa     2160

cuccguagaa aguggggacu ggaaaagucc agggagacuu accaccauca cgccguagau     2220

gcauuaauca uagccgcuuc uucgcagcug cgccuuugga aaaagcagaa uaaccccuug     2280

auuaccuaua aagaagguca auucgucgac agcgagacag gcgaaaucau uucccucacc     2340

gacgaugagu acaaggagcu gguguucaaa gccccuuaug accauuuugu ugauacucua     2400

cggucaaaga cauucgaaga caguauccug uuuucuuacc agguggauag caaauucaau     2460

agaaagauau ccgacgcaac gaucuacgcg accaggaagg cuaaacucga uaaggagaaa     2520

aaggaauaua cuuacacacu ggggaaaauu aaggacaucu auucacuugg aaccaagaca     2580

ccaagcaaaa cuggcuuuua caaguucuug gaucuguaua acaaagacaa gucucaguuu     2640

uuaauguucc agaaagaucg aaagaccugg gacgagguca uugaaaagau cauggagcaa     2700

uaccgccccu uuaaagagua ugauaagacg gguaaacucg uggacuucaa cccuuuugaa     2760

aaguaccggc aggagaaugg gccaauaugu aaauacucca agaagggcaa cggacccgaa     2820

auuaaaaguc ugaaguauua cgacauccuu cugggcaaac acaagaauau caccccggag     2880

gggagcagaa acacaguagc cuugcucucu cugaauccuu ggaggacuga uguguauuac     2940

aacauggaaa ccaaaaagua ugaguuccua ggacugaagu acgcagaccu ccccuuugag     3000

aaagguggcg ccuaugggau uuccacagaa acuuacaaua agcugcguga gaaagaagga     3060

aucggcaaga acucagaguu caaauucacc cuuuacaaga augauuugau ucugauaaaa     3120

gacacggaga caaacugcca gcagaucuuu cgcuucugga gucggaccgg uaaggauaau     3180

ccaaagagcu uugaaaaaca uaagauugag uuaaaaccuu augaaaaggc uagauucgag     3240

aaaggggagg aacucgaggu ucugggcaag gugcccccau cguccaacca auuucagaag     3300

aauaugcaga ucgaaaaccu uucuauuuac aaagucaaga cugacgugcu gggcaaaaag     3360

cacaucauca aaaaggaggg agaugaaccc aagcuaaaau ucuga                     3405


<210>  2834
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2834
augaaguaca ucgagagcgu guccauuggc uucgacauag gggucggauc uguugguuau       60

gccgugauca acucaaaaac caagcagauu cuggaaagug gcguaagcau cuuucccgcu      120

gcaguggccc agaaaaaugu cgagagaagg gaaucccggc aaucucgccg acuccguaga      180

aggcggguga accgccuuaa ugaucugaag aaauuguuaa agaagaacgg guucauuccu      240

ucgccaaaua aaaacccgua cgagaucaga guuaggggac uggaacacca gcuaucacgg      300

gacgagauag cgaucgcugu gcuccauauu gccaagcgcc gaggcaucag cuauagucug      360

gaugacgucg uggaugagaa uguaacagac uuuaaaggua agauugccaa aaaccaggaa      420

gcacuuaaga auaagacucc cggggagauc caauacgaaa gacuguugaa auauggaaag      480

guuaggggca cggugaccaa cuacgucgag uaugaugagc agccugaaua cuuccucaau      540

guguuuccaa acuccgcuua cguggacgag gcaaaacgga uacugacaug ccagaagcag      600

uauuaccccg aaauuacuga ugaccugauc gagaauauug ucaaaaucau cucucguaag      660

cgcgccuauu acauuggccc ugggaacgag aaaagcagaa ccgauuaugg aauauacagg      720

acaaauggug aaacccucga gaacaucuuc gaaauuuuaa ucggcaagga cuccguuaac      780

ccagaugagc ugcgggcucc cgccaauuca cuuacugcgc aaguguauaa cuugcugaau      840

gaccuaacaa acauacgaua caaugggagu gaacgccuca ccccugagca gaagcaggac      900

auucugagcg aacuucugac gacugagaaa accguacaga uguugaacgu gaucgcaaag      960

aaagucaaug ugaagaaaga ggaaaucucu ggauuuagaa caaagaagga ugacaaacca     1020

gauuuccaca cucucgaggu uuacaggaag gugcggaaac auaugcugaa caagggcauu     1080

gacaucaaug aauggccgac cgaguguuuu gaugaauuag ccuucauuuu uaaccugaau     1140

acagagauag gugagauccg caacagaauu uccgaaaaag ucgugcccaa guaugacuuc     1200

aucaccaauc aaaucguaga ugaccuccuu gaggauccua agauuuuugc uauaucaacg     1260

agccagaaau ggcacagguu caguaucaag acuaugaacg uucugauucc agaauugcua     1320

aauggguaca acgagcagau gacaauccug acccguaugg gacuccugga ggacucuaaa     1380

aacgauuauu ccaauauuga caagaucgac aucaaaaacg ugacugaaaa uauauacaac     1440

cccgucgugc gaaagagcau uaagaaaugc cuuaagaucu ucaaugagcu guauaaaaag     1500

uacccuaaca uuucggaugu cgugaucgaa uugccacggg aggacaaucc cgaagaugcc     1560

agaaaacuca uaaagaaaau gcaaaaggag aaccugguug agaaggacac aucuuuagaa     1620

gcauuuagga aagaggcuaa ucuguccgaa aacggccuaa uugauuuccu ccgcaagggc     1680

aaaaaucggg cccugaagau cagacuuugg uaucagcagg aggggaaaug uuuguacacc     1740

ggagaaccga uucuggcgga ggaccucugc aagaacgagg ccaauuacga uaucgaccau     1800

auaaucccua uuucagugag uuuugaugac agcaucaaca auaagguacu guguuccucu     1860

cacuucaaca gguuugaaaa agagaagaaa acgcccuucg aauauuuuag caacaaguca     1920

agucaccuug gucgcuacuc caauuucgau gaguuucgag caagcgucaa cgcuaauaaa     1980

cggauuucug acgugaagcg uaagaaccug uugucaaaag aacuccugag ugauuuagac     2040

accagaaaga aauucaucgc caggaaucug guugacacac gcuaugcuag ccgggugguc     2100

uuuaacgagc uaacucaguu cuuugccucc aagaaagcag auaccaaggu ggccguaaua     2160

agaggcaagu ucacaucuca acucaggcga gcuuggcagc ugaauaaauc gcgcgacacu     2220

uaccaucacc augcggugga ugcagcccuu auugggguua ccccauucuu gaagaucugg     2280

cggagcggaa aaacaaucua uccuagaaag augaccgagg cucagacuga cuuugaacug     2340

aacgagauua cgacaaauga ugaguucaaa gaauccuacu aucagcuccc cugcccacaa     2400

uuucuggacu acuuaaccca gaugcaggag aaccugaaga ucaauuucga agucgauaaa     2460

aagaugaaca ggaagauuuc agacgccaug auauacaguu cucguaaaaa gaaacuuagc     2520

aaggauaaaa aggagaagga auauuccuug ggcacuauca augacauuua cucagaugca     2580

gguuauagca aauuuaagaa caucuacaaa aaggacaaag agaaguuccu cauguaucac     2640

cuggaucaca agucuuuuga aauucuagag gacaucauau cccaguaccc cgacaaagag     2700

gaauauaucg auagugaggg gaaggugaaa caacgcgccg ugagcccuuu cgaaauuuac     2760

cggagaaagu acggcuauau caggaaauac ucuaagaaga auaacggacc aauuaucaaa     2820

aacaugaagu auuacaauga gaaccugccg aaaauauaua uugacaucac ccccaagcag     2880

uccaucgaua aaaaggucua ccucacaaau auuucaccuu ggcgaaccga cguuuauuac     2940

aaccggaguc agaauagcua ccauauaaug ggcaucaagu auucggaucu gcgcuacauu     3000

gacgggaaau auggaauccc aaagaacgug uacgaaaaga uuaaacuuag agagaaaauc     3060

cccuccgagu cugaauuuug uuucagcuug uauaagaaug auagggugaa aauaaucgac     3120

ccuaacaaga augaggaaau ugagcugcgg uuuaacucac gaacuuuacc aaguguacuc     3180

aauuacgucg aguucaagcc ccuguuucag gaauccuaua acucucuuac acuguugccg     3240

guguucagca aaacgaccuc cgaggguaga aucauuaaga aauucacucg cgaagauaau     3300

aucguuuaca aggugaacac agacgagcuc ggcaauccuu acuauauacu gaaagaauca     3360

caaaacccaa agaauauuau cgauaacagc aacaagaccg aguga                     3405


<210>  2835
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2835
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgagggugu cggcauacug       60

gaaaagaacu ccgggaaaau cauucacgcu aauucuagau uguuccccgc agccaccgcg      120

gauaauaacg uugagaggcg gaagaaucgc caggcucgac guuuaaacag aaggaaaaag      180

caucggggag ugcgcuuuca agaccuguuc gaagauuacg gccuacucac agacuuuuca      240

aaaguaagua ucaaucugaa cccuuauaga cuuagggugg auggucugaa ucagcaguug      300

acuaacgagg aacucuucau ugcccugaag aauaucguca agcggcgcgg gauaagcuac      360

cuggacgaug cauccgagga cggaggcgcc gugucuucgg auuauggcaa agcuguugag      420

gaaaaccgaa agcucuuagc agagcagacg ccagggcaaa uccagcugga aagauuugag      480

aaauacggac aggugagggg ugacuucacc gucguggaga auggcgaaaa acuucgguug      540

auuaacguau uuucaacaag cgccuauaaa aaggaggcug aacguauccu gcgcaagcag      600

caagaguuca auaaccagau uacugaugaa uuuaucgagg acuaccuaaa aauacucacc      660

gggaagagaa aauauuacca cggaccgggc aacgagaaga guaggacaga uuacggucgg      720

uuccgaaccg acgggacuac acuggacaau auuuuuggaa uccuuauugg caaaugcacc      780

uucuaucccg aagaguaccg cgccuccaag gcgucuuaua cggcacagga auuuaaccug      840

uugaaugauc ucaacaaucu gacuguuccu accgagacaa agaaguuaag cgaggaacag      900

aagaaaguga ucgucgagua cgccaaggaa gcuaaaacuc ugggcgccuc cacccuccuu      960

aaguauaucg caaaaaugau ugacgcuuca guggaucaaa uaagagggua caggguggac     1020

gucaacaaua agccagagau gcauacauuc gaaguuuauc ggaagaugca gagucuggag     1080

accaucagcg ugggagaauu gucucgcaac guacuagaug agcuggccca cauucucacg     1140

cugaauacug agagagaagg uauggaggaa gcgaucaaca caaaacuuaa ggacuccuuc     1200

ucacaggauc aagugcugga guugguccag uuuaggaaaa auaacagcag ucucuucucu     1260

aagggcuggc auaauuuuuc ccugaaauua augauggagc ugauacccga acuauacgag     1320

accagcgagg aacagaugac uauucucaca cgucugggga agcagaaguc gaaagaaacc     1380

ucuaagcgga cgaaauacau cgacgagaag gagcuuaccg aagagaucua uaacccugug     1440

guugccaaau ccgugcgaca agcaauuaag aucauuaaug aagcuacaaa gaaacacgga     1500

auauucgaua acaucgucau ugagauggcc agagaaaaca augaggagga cgcuaagaaa     1560

gauuacauca agaggcagaa agccaaccag gacgaaaaga augcagccau ggagaaagcu     1620

gcguuucagu auaacggcaa gaaggaauug ccagacaaua ucuuccacgg ucauaaagag     1680

cuggcaacua agauucgccu cuggcaccaa cagggggaaa aaugucugua caccggcaag     1740

acaauaccca ucucagaucu uauuuauaac caguacaaau augagaucga ccauauucug     1800

ccuuugaguc ucagcuuuga ugacucccug ucuaauaagg uguuagcccu ggcuacugcc     1860

aaccaggaga agggacaacg gaccccauuc caggcacuag auagcaugga cgaugccugg     1920

ucauacagag aauuuaaaag uuacguaaag gaccucaaau cccugagcaa uaagaaaaag     1980

gauuaucuuu ugacagagga agacaucucu aagauagagg uuaaacagaa guucaucgag     2040

aggaaccugg uggauacccg cuacucaagu cgagucgugc ucaaugcucu gcaggacuuu     2100

uauaaaagcc accaauucga uacuacgauu uccgucgugc ggggccaguu uacaucucag     2160

uuacguagaa agugggggcu ggaaaaaucg agggagaccu accaccauca cgccguugac     2220

gcacuuauca uugcggcuag cucccaauug cgccucugga agaagcagaa caauccgcug     2280

aucacuuaua aagaaggaca guucguggac ucacagaccg gugagauaau uagucuaaca     2340

gaugacgaau acaaggagcu gguauucaaa gcccccuaug aucauuuugu cgacacccuc     2400

ucuagcaaga cuuucgagga uuccauccug uuuucauacc aaguggacag caaauucaac     2460

cggaagauuu cugaugcaac aaucuacgcc acgagaaaag cuaaacuuga caaggaaaag     2520

aaggaguaua ccuacacuuu gggcaaaaua aaggauaucu auucccuggg gacaaaaacc     2580

ccuaguaaga caggauuuua caaguucuua gaccucuaua auaaagauaa gagccaguuu     2640

cugauguucc agaaagacag gaagacuugg gaugaaguua uugagaaaau cauggaacag     2700

uaccgaccau uuaaggagua ugacaagacc ggcaaacuug uggacuucaa ccccuuugag     2760

aaguaccgcc aagaaaacga gccuauucgg aaauacucua agaaagguaa ugggccagaa     2820

aucaaguccc ugaaguauua cgauauauug cucggcaaac acaagaacau uacgcccgag     2880

ggaucaagaa auaccgucgc ccugcuaagu cugaacccgu ggaggacaga cguguauuac     2940

aauauggaaa cuaaaaagua ugaguuccuc ggccugaaau acgcagaucu uccuuuugag     3000

gaagggggag cuuaugguau cagcaccgag acauacaacg aauugcguga gaaggagggc     3060

aucgggaaaa auucggaguu caaauucacu cuguacaaga acgacuuaau ucucaucaaa     3120

gauaccgaaa cgaauugcca gcagauuuuu cgcuucuggu cccggacagg aaaggacaac     3180

cccaaaucuu uugaaaagca uaaaauagag cugaagccau augaaaaggc cagauucgag     3240

aaaggcgagg aacuugaggu acuggguaag gugccuccca gcucaaauca gcuacaaaag     3300

aacaugcaga ucgaaaaucu caguauuuac aagguuaaaa ccgauguccu ggggaacaag     3360

cacuuuauca agaaagaggg cgacaagcca aaauugaagu ucuga                     3405


<210>  2836
<211>  3408
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2836
augagcgacc uggugcucgg ccuugauaua gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu uggagaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggcgcg uaagacugaa ucaccuauuu gaagagucag gccucauuac ggacuucacc      240

aaggugagua ucaaccugaa uccuuaccag cuuaggguca aaggucugac agccgaauug      300

agcaacgagg agcucuuuau agcgcugaag aauaugguga agcaucgggg gaucuccuau      360

cuggaugacg cuucugauga cggaaacucg ucaguuggcg auuacgccca gauugugaaa      420

gaaaauagca agcagcucga gacuaaaacc ccaggccaaa uccaguuaga acgcuaucag      480

aaguacgggc agcugcgagg agacuucaca gucgaggagg augguagaaa acacaggcuu      540

auuaacgugu uuccgaccag ugcauaucau gccgaggcuu ugcggauccu gcaaacucag      600

caggaguuca auccccagau aacagacgaa uuuauuaacu ccuaccuaga gauccucacc      660

ggcaagcgua aguauuacca cgggccugga aaugaaaaau cucgcacgga uuacggcaag      720

uauacuacca aaaaggacgc acaaggucag uacauuacac ugaacaacau cuucgggauc      780

cuuauuggaa aaugcacuuu uuauccagag gaguacagag ccgcuaaggc cagcuauacc      840

gcgcaggagu ucaaucuguu gaacgaucuc aauaaccuga caguacccac cgaaacgaaa      900

aaguuauccg aagaacaaaa guaccagaua aucacuuaug uuaaaaauga aaaggcaaug      960

ggcccugcca aacuguuuaa guacauugcu aaacuccuug ccugugacau cgcagacaua     1020

aagggcuaca ggauugauaa gucagacaaa gcugagaucc acacauucga ggccuaucgg     1080

aagaugaaaa cccuggaaac uaucgauauu gagcagaucg aacgagagaa guuggacaaa     1140

cuagcguacg ugcugacacu caacaccgaa cgcgagggga uucaggaggc ccuggaucau     1200

gaauuugcag acggaacguu cagucaagag caggucgaug aacuugugca guucagaaag     1260

gcuaauagcu cuauauuugg uaagggcugg cacuccuucu cagugaaacu gaugauggag     1320

uugaucccag agcucuaugc caccagcgag gaacagauga caauucugac uagguuaggg     1380

aagcaaaaga ccacaaguuc uuccaacaag acuaaauaca ucgacgaaaa gcagcugacc     1440

gaggaaaucu auaaucccgu cguugcuaag agcgugcggc aggccauuaa aauaguaaac     1500

gcagccauca agaaauacgg agauuuugac aauauuguga ucgagauggc ucgcgagaca     1560

aacgaagaug acgagaagaa agcgauucag aagauccaaa aagcaaauaa ggccgaaaag     1620

gacgcugcca ugagaaaagc agccaaccag uauaauggca aggcugagcu accucauucg     1680

gucuuccacg gucacaaaca gcucgccacc aagauaaggc uguggcauca gcaaggggaa     1740

cguugccuuu acacuggcaa aacgaucucu auucacgauu ugaucaacaa cccaaaucag     1800

uuugagauug accauauccu gccgcucucc auaacauucg augacucacu ggcaaacaag     1860

gugcuuguuu acgcgaccgc uaaucaggag aagggacaac ggacucccua ucaggcccug     1920

gauaguaugg acgaugcaug gagcuuucga gaauugaaag ccuucgugag agaguccaag     1980

gcucucucua acaaaaagaa agaauaccug uuaaccgagg aagacauuag caaguuugau     2040

gucaggaaga aauucaucga gcgcaaucug guggacacac gguaugccuc aagaguaguu     2100

cuaaacgcac uccaggagca cuuuaaggcu cauaaaauug auaccaaggu gagugucgug     2160

aggggccagu ucacuuccca acugcgccga cacuggggga ucgacaaaac acgggauacg     2220

uaccaccauc acgccgucga cgcgcuuaua aucgccgcua gcucucaguu gaaucugugg     2280

aagaagcaga aaaacacccu cgugucauau agugaagacc agcuguuaga uauugagacu     2340

ggagaacuga ucagcgacga ugaguacaag gaguccguuu uuaaagcacc uuaucaacau     2400

uucguggaca cacuuaaguc uaaagaguuc gaagauucga uuuuguuuag cuaccaggua     2460

gacuccaagu ucaaucguaa aaucucagau gccaccauau acgcuacaag acaggccaag     2520

gucgguaagg acaaaaagga ugaaacuuau gugcucggca aaauuaagga caucuacgca     2580

cuggggacca aaacgccaag uaagaccgga uuuuauaagu uccuagaucu guacaacaaa     2640

gacaagucuc aguuucucgu uuaucaaaaa gauaggaaga caugggacga ggugaucgag     2700

aaaauuaugg aacaguaccg ccccuucaag gagagcgaca agaauggcaa agaagucgau     2760

uuuaacccuu ucgagaagua ucggaucgaa aaugguccaa uuagaaaaua cuccaggaag     2820

gggaacggcc ccgagauaaa aucacugaag uacuaugcga gcaaacuugg aaaucacauc     2880

gacauuacuc cgaaagauuc uaacaacaag gugguauugc aguccgugag uccuuggcga     2940

gccgacguuu acuuuaauaa aaccacaggc agcuaugaaa uccugggguu aaaguacgcu     3000

gaucuccaau ucgagaaagg aacugguacc uaucgcauuu cugaggaaaa guacaacgac     3060

aucaagaaaa aggagggcgu cgauuccgac ucagaauuua aauucacgcu guauaagaau     3120

gaucuucugu ugauaaaaga cacagagacc aaggaacagc ggcucuuuag auuccugagu     3180

aggagcaugc ccaacaaaaa gcacuacgug gagcuaaagc cauacucgaa agauaaguuc     3240

gagaaaaaug aaucccugau cgagauucuc gggucugccg acaagagcgg acguuguauc     3300

aaaggccugg caaagucaaa ccuuaguauu uuuaaggugc gcacugacgu cuuggguaau     3360

cagcauauca ucaaaaacga aggggauaag ccuaaacugg acuucuga                  3408


<210>  2837
<211>  3408
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2837
augggcguga ucuuccugua cgagaacauu gucaagcccu uuccuagcag auaugacaaa       60

gccauaauga agaggcucaa agggcggcag accaugggau acauccuuuc ccuggauauu      120

gguaucgcuu cuugcggcua uucaguuuug gaaacagacg cauuaggggu gccauacaag      180

auuaaaacuc ugaauaguau cauauucacg cgcgccgaga acccgaagac cggagcgagc      240

cuagcugaac cccgacguaa guauagaggc gccaggcggc gcaaaagaag gacauccuuu      300

cggaagcacc gcacucgaca acucuucauc accucugguc ugcuuucgaa acaggauauu      360

gcaagauacc ugaaucagca ggagccucau gacgauaucu gggaauugag gguaaagggg      420

cucgacgagc cacugacaaa ccugcaacuc uuucaggugu uauauuacuu cgucacccac      480

cggggauuua aaucaaaucg uaaggccgag cugaaggaua aagacauugg ccccgugcuu      540

aacagcauca augcuuucaa ggaagagaug cagagucaug auuaccgcac uauaggcgaa      600

uuguaugcaa acgacccuag auuuagggau cggaaacaca auaaggacua cacaaacggg      660

uuccugggaa ccauucagcg auccuggcua guugaugaga ucaaaaugcu ccugacgaau      720

caacagucuu uugguaacac ucagcuuacc agcgaguuca uugaacugua uuugaauauc      780

uuuacauccc agcgcaacuu cgacgacggc ccagccucag ggcccuacag uggagaucuc      840

aucgacaaga uggugggccc ugauacuuuc aauagaagca ggaaacgggc uccacgcgcc      900

accuauacau uucaauacuu cucuuuucug uccaaguuaa acucacugag aauugcgccg      960

agcauaagug cacccuucga gacccucacg gucgaccaga gggcccuuau cacuaagcgu     1020

cugugggguc cugaugugaa aucugcugac uugaaguuua aacagauucg gaugguacua     1080

ucccugccag aaacagcccg auucaaccuc guuaauuaug ggaacacccc cgagaaggca     1140

cuggaaaucg aggauaaaac uacauuuaua agcauuaagc cucuuagaga gaucgaaaag     1200

gcucugaccg gaacgaccuu gccagugucg caugaccaac ucgaugccau cgcgaggauu     1260

cugacagucu acaaaucuga cuccaaucgc aagacugccu uccacaacca gaccguguua     1320

ucagaugagg ugaucgaaca gcugcuaccc cucaauuuua guaaauucgg ccaccugagc     1380

gucgagacaa ugcaggaaau uauaccguac cuugagcaag gcaugguuua uuccgacgca     1440

gcuacugccg cuggguacga cuuucggaac aaucagaucg auauggagua uauuagagac     1500

aacgugacca auccuguagu gaggcgcgcc gucucugugg caaacaaguu gguuaaagcc     1560

gugguccgac gguacggaaa gcccgauagc auccauaucg aacuggcucg ugaccucuca     1620

cagaauuucc aggagagaag gcaagaugaa gugcgccagc agaagaacgc gcgggacaau     1680

gagaaaauug caaagagacu ggaggaacuu gguauaccag uaaacggcgu uaauaucaca     1740

aaaaugaagc uguaugauga gcagcacggg auugacuugu acgaucucag uaccaacgcc     1800

uccauggcug ugacugacau cuuuagcgau gacagguaug aaauugauca uaucauaccu     1860

uacucugucu cauucgacga caguuacacg aacaaagugc uggucagcgc caaggcaaau     1920

cgagagaagc gcgaucggau ccccauggaa guguucggaa acgacuccga gagaaggcgu     1980

caauuugaga aucgcguuaa cacaaauauu accaacuuac ggaaaaagag aaaucugcua     2040

aaagaaacuu ucucugauga ggacaaggcc aaauggaagu cgaggaacau caaugauacc     2100

cgauaucuca accggauucu gaugaauuac cuuagccaga acguggaauu uacagacgua     2160

uuguccgaug cuaaacacgu caucgcccug aauggcgcag ugaccgcgaa gauacgcuca     2220

agauggggua uuacuaaggu uagggcugcc ggggaccucc accaugcagu ggaugccguc     2280

gugaucggcu guguaacaga cgcucugauu caggccguga cgcgguaugc aaaauaccag     2340

gagcgaagag auaacaaggc ugccgcggaa caagacuuag ccgagaauaa cccagaggaa     2400

cuggaugcua aacaguuccg caagguuuuu aacgacuauc cccuuccuug gccagaguuc     2460

agggaagagu ugaugggacg ugucaguucu gacccgagcc agaucauggc aggccggcaa     2520

uggucccacu acaccgccga agagauauca gagcucaaac agcccuuugu ggugagaguc     2580

ccuaaucaua ggaucguugg gccagcuacu cuggauacag uguauggaca gcccaacccu     2640

gacgaugguc uaauuaccca gcgcgugcga aucacagacc ugaagccaca aaagaauaaa     2700

gauggcgaag acauuaucaa ggccggggca aacguauacg agcucgcggc cgauggaggc     2760

aauaaacugg ucuacgaaag cauacuugac gcuuugcaag uggccggugg ggauggcaag     2820

aaagcauucc ccacuggaag acuguuaguu cggucuccga uucaggacau cccuguggag     2880

aggguccgcg ugaccuccaa ggcuacgcuc guugugccag uaggccccaa gagugucgcc     2940

gcaaacggga gcauggugcg uaucgaugug uauaaaaccc cuaagaaaua cgucuuuguu     3000

cccauuuaug ugagagacac aguaaaggcc gaacugccag accgggcugu ggcgccuguc     3060

aaacccuacu cucaguggua ucagcuucag ccaaaggauc aguuccuguu uuccuuguuc     3120

gccaaugacc ucaucagguu uaagcacccg cgcggagugu caacuaaaga gauucugacc     3180

gaugguaguc aggagaaggg caaacgaaca gacguugugg gguacuucac uggagcagau     3240

auagcuaccg ccagcaucgc cauugaaggc ccugacagau cguucuccgc ucgggguauc     3300

ggguuugcau cucuaaagaa cgucgagaaa cugacgguga auuauuucgg cgaacuccau     3360

agcguucacg agacacaacc caugcaguuu aggggacagc gcggcuga                  3408


<210>  2838
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2838
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaggaua ccgggaaaau cauucacgcu uccucuagau uguuccccgc agccacagcg      120

gacaauaacg uggaaaggcg gucaaaucgc cagggacgac guuuaaacag aaggaagaaa      180

caucggagug uacgccugca agaucuauuu gagagcuacg gccuccugac ugacuucucc      240

aaacuuucua ucaaucugaa cccuuaucag uugagagugc aggguaugga aaaucagcuc      300

acgaacgagg aacuguuuau ugcucugaaa aauaucguca agaggcgggg gauaucguac      360

cucgaugacg ccucagagga uggaggcacc gugagcagug acuauggcaa ggcaguugag      420

gaaaaccgcc gauuacugac agagaaaacu ccagggcaaa uccagcuuga aagauucgag      480

aaguacggac aguugagggg ugauuuuacc guggaggaaa auggcgagaa acggcgucug      540

auuaacgucu ucuccacauc ugccuaucgc aaggaagcug agagaauccu aaggaaacag      600

caagaauuua auaacaagau uaccgacgag uucaucgagg auuaccucac uauacugaca      660

gggaagcgga aauauuacca cggaccgggc aaugaaaaga gccgaaccga cuacggucgc      720

uuuagaacgg augggacuac ccuugacaac auuuucggaa uccugauugg caaaugcaca      780

uuuuaucccg aggaauacag ggcauccaag gccucauaua cugcucagga guucaacuug      840

cucaaugacc ugaacaauuu aaccgugccu acagaaacca aaaagcugag ugaggagcag      900

aaaaagacga ucaucgaaua cgccaagagc gcgaaaacuc ucggcgcauc uacacuucug      960

aaguauauug ccaaaaugau agaugcuucc guagaccaga uccgggggua ccgcguugau     1020

gugaacaaua agccagagau gcauaccuuu gaagucuaua gaaaaaugca aucauuggag     1080

acuauuaagg uggaagaacu acccaggaag gugcuggacg agcucgccca cauccugaca     1140

cuuaacaccg aacgugaggg aauagaagag gcaauuaaua gcaaacugaa ggauacguuc     1200

aaccgggacc aggucuugga gcucguucag uuccgaaaga auaacaguuc ucuguuuucc     1260

aaggguuggc auaauuucag cuuaaaacug augauggaac uaaucccuga gcucuacgaa     1320

accucggagg aacaaaugac aauccugacu agacuuggca agcagagguc uaaggagacc     1380

uccaaacgca caaaguacau ugaugagaaa gaauugacug aggaaaucua uaacccagug     1440

guagcuaagu cagugcggca ggccauuaaa auaaucaaug aggcgaccaa gaaguacggg     1500

auuuuugaca acaucgucau cgaaauggcc agagagaaua acgaggaaga ugcaaagaag     1560

aacuauauua aacagcaaaa ggcuaaucag gacgagaaaa acgccgcuau ggaaaaggcc     1620

gcauuccagu acaauggcaa aaaggagcug cccgauaaca uauuucacgg acacauggaa     1680

cucgccacaa agaucaggcu guggcaucag caaggugaga aaugucuuua uaccgggaag     1740

aauauucaga ucagugaccu gauucacaac caguacaaau augagaucga ccauauauug     1800

ccucucagcc uguccuucga ugacucuuua agcaauaagg ugcugguucu agcuacugcg     1860

aaccaggaaa aaggccaacg cacgccauuu caggcacucg auucaaugga cgaugccugg     1920

aguuaccgag aguucaaguc cuacgugaag gaaagcaaac ugcuuggaaa uaagaaaaag     1980

gacuauuugc ugacagagga ggauaucucu aaaauugaag ucaagcagaa guuuaucgag     2040

aaaaaccucg uggacacccg guacucaagu cguguaguuc ugaaugcuuu acaggauuuc     2100

uauaagaaac accaauuuga cacuaccauu agcgugguca gaggccaguu cacaucccag     2160

cugaggcgca aguggaaacu ugaaaagucu cgggauaccu accaccauca cgccguggac     2220

gcauugauca uagccgcuuc gagccaacuc agacugugga agaaacauaa caauccgcua     2280

auuuccuaua aggaugggca guuugucgac ucagagacug gagaaaucgu gagucugucu     2340

aacgaggagu acaaagaacu cguuuucaag gcccccuaug accacuucgu ggauacacug     2400

aggagcaaaa aguuugagga cuccauucuu uucucauacc agguagauag caaauacaau     2460

cgaaagaucu cugacgcaac gauauaugcg acccgcaagg cuaaauugga uaaggacaaa     2520

aaggaauaca cuuauacacu ggguaaaauc aaggauauuu acgccuuagg caccaagaca     2580

ccuuccaaaa cuggguuuua uaaguuccuc gaccuguaca aaaccgauaa gagucaguuu     2640

cuuauguauc aaaaagaccg gaagacgugg gaugagguca ucgaaaagau uaucgagcag     2700

uacagaccau ucaaagagua cgacaagaac ggaaaagaag uggauuuuaa ccccuucgag     2760

aaguauagga uagaaaaugg cccuauucgu aaauacagcc gcaaggguaa cgggucuguu     2820

aucaagucca ugaaauauua cgacaaucug uugggcaagu uuguggacau caccccauca     2880

gagaguaaaa accccgucgc acuccugagc cuaaauccgu ggcggacaga uguguauuac     2940

aacccugaaa cuagaaagua ugaguuccug ggacucaaau acgccgaccu gugcuuuggc     3000

aaagggggau cguacgguau uuccgaggua aaguauaaua aaaucaggga aaaggagggc     3060

auugggaaag aaucugaguu caaguuuacc cuuuacaaaa acgauuugau acugaucaag     3120

gacacagaga cuaauuguca gcaguucuuc cgauuuugga gccggaccgg aaaagauaac     3180

cccaagucau ucgaaaagca uaaaauugag uuaaagccau augaaaaagc uaaguuugag     3240

aaaggcgagg agcucgaggu gcuggguaag guuccuccca guuccaauca auuccagaag     3300

aacaugcaga ucgaaaaucu uucuauuuac aaagucaaga cggacgugcu ggggaacaaa     3360

cacuuuauca agaaagaggg cgaugaacca aagcuaaagu ucaaaaagug a              3411


<210>  2839
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2839
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

aagaaagaua ccggggagau cauucacaca aauuccagau uguucucugc ugcaacugcc      120

gacucaaaca ucgaaaggcg gcgccaucga ggaggcaagc guuuaacgag aaggaaaaag      180

caccggagua uucgccugca ugaucuauuu gaggacagcg gucuccugac cgauuucucc      240

aaagugucua ucaaucuuaa ccccuaccag cugagaguac aaggguugga caaucagcuc      300

acaaacgaag aacuguuuau agcgcugaag aauaucguga agaggcgggg aauuucguau      360

cucgaugacg cuucagaaga uggcggcacu gucagcagug acuacgggaa agccguggag      420

gagaaccgca aguuacuggc agaacagacc ccuggacaga uccaacuuga ucgauucgag      480

aaauaugguc agguuagagg cgacuuuaca guggucgaaa auggggagaa gaggcgguug      540

auuaacgugu ucaccacuuc cgccuacucu aaagaggcug aacguauccu gcgcaagcag      600

caggaguuua auaagaaaau aacagaugag uucauugaga acuaucuaac cauccucacg      660

ggaaagagaa aauacuauca cggcccaggu aaugaaaaga gcaggacuga cuacgggcgg      720

uacaccacua aaaaggaccc ggagggaaaa uauauuacac uggauaacau cuuuggcauc      780

cuuauuggca agugcaccuu cuaccccgac gaguaucgag cauccaaggc cucauacaca      840

gcucaagaau uuaaucuguu gaacgaucuc aacaaucuga ccguaccuac ggagacuaaa      900

aaguuaagug aggaacagaa aaagacaaua aucaaauaug ccaagaccgc gaagacucug      960

ggggcaagca cacuccuuaa auacauugcc aagcugaucg gagcuucugu ugaccagaua     1020

caugguuauc gcauugaucc aaacaaaaag cccgaaaugc acaccuucga gacguacaga     1080

aaaaugcagu ccuuggagac caucucagug gaagagaucc cuaggaaggu ccuagacgaa     1140

cuggcccaca uucucacacu gaauacugag cgggaaggca ucgaggaggc aauuaacgcu     1200

acccuuaagg auacauuuag ccaagaccag gugcuggaau uggugcaguu ccgcaaaaau     1260

aacaguucuc ucuucuccaa gggguggcau aauuuuagcc ugaaauuaau gauggagcug     1320

auaccagaac uauacgagac uucggaggaa caaaugacca uccucacaag acugggaaag     1380

cagaaaucua aggagaccuc caagaggacu aaauauauug augaaaagga gcuuacggaa     1440

gagaucuaca accccgucgu ugccaaauca gugcgucagg cgaucaagau uauaaaugag     1500

gccaccaaaa aguauggcau cuucgacaac auuguaaucg aaauggcacg ggagaauaac     1560

gaagaggaug cuaaaaaggg uuacaaugaa cgacagaagg ccaacuugga cgagaaaaag     1620

agugcuaugg agaaagccgc auuucaauau aaugggaaga aagaacugcc ugacaacgug     1680

uuccacggcc auaaggagcu cgccacaaag auuagacugu ggcaccagca gggagaaaaa     1740

ugucuuuaca cuggcaagaa caucccaaua agcgaucuga uccagaauca auauaaauac     1800

gagauugacc acaucuugcc gcucucccug ucuuuugaug acagcuuauc aaacaagguc     1860

cuggugcuag cuaccgcgaa ucaggaaaaa gggcagagga cacccuucca ggcacucgau     1920

aguauggacg augccugguc cuaccgcgag uuuaagagcu auguuaagga ccugaaacuu     1980

uugucuaaca agaaaaagga uuaccugcuc accgaggaag acauuucaaa aaucgaggug     2040

aagcaaaagu ucauagaacg gaaucugguc gauacuagau auaguagcag ggugguauua     2100

aacgcucugc aggacuuuua caaagagcau caguucgaua caacgauuuc cguugugcgc     2160

ggacaguuua ccucucaacu ucgacggaag ugggguuugg agaaaucgcg ugaaacuuau     2220

caccaucacg ccgucgacgc acucaucauu gccgcuagcu cccagcugag acuauggaag     2280

aaacagaaua acccucugau cucauacaag gagggccaau ucguggacca ggaaacaggg     2340

gagauaauca gucucucuga ugacgaauau aaagagcugg ucuuuaaggc cccauacgau     2400

cauuucgugg acacccuuaa gagcaaaaag uucgaggauu ccauuuuguu uucauaccag     2460

guugacagca aauauaauag gaagaucucu gaugcaacaa uuuacgcgac ucgcaaagcu     2520

aagcuggaca aggagaaaaa ggaauauacc uacacguuag gaaaaaucaa ggauauauau     2580

gcccucggcu ccgaaaaacu gaagaccggu uucuacaagu uucuucaccu guauaaaaag     2640

gacaaaaguc aguucuugau guaccaaaag gaucggaaaa caugggacga ggugauugaa     2700

aagauccucg agcaguacag acccuuuaag gagaaagaua agaacgggaa agaaguagac     2760

uucaauccuu uugagaagua uaggaucgaa aacggcccaa uucacaaaua cagccgaaag     2820

ggaaauggcc ccgucaucaa gucucugaaa uauuacgaca agaacauucc ggaguccuuc     2880

auaaauauca cuccuucaaa caguaaaaac aaggugauuc uacgccagcu gaaucccugg     2940

cggaccgaug uuuauuuuaa ccaucagaca gaaaaauacg agcuccuggg gcuuaaguau     3000

gcacacuugc aauucgagaa aaagacugga aauuacagca ucaccauuga caaguacaac     3060

gaaaucaaag agaaggaagg ugugucggag caguccgagu uuaaauucac gcugcauaag     3120

aaugauuuac uccugauaaa agacacagaa accaaggagc agcagcuuuu uagauuccug     3180

ucuaggacua agccaaacgu caaacacuau guggaacuaa agccuuacag ccgugaugag     3240

uucgagaaaa augaacgccu caucgagauu cugggcucag uagacaagag ugggcgguuu     3300

gugaaaggau ugggcaaguc caaccugucu aucuauaagg uuagaacaga ugucuuaggu     3360

aaucaacaca uuaucaaaaa ggaaggggag gaacccaaac ucaaguucug a              3411


<210>  2840
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2840
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaggaua ccgggaaaau cauucacgcu uccucuagau uguuccccgc agccacagcg      120

gacaauaacg uggaaaggcg gucaaaucgc cagggacgac guuuaaacag aaggaagaaa      180

caucggagug uacgccugca agaucuauuu gagggcuacg gucuccugac ugacuucagc      240

aaggugucca ugaaucuuaa cccuuaucag cugagagucc aggggaugga aaaucaguug      300

acgaacgagg aacucuuugu ggcucugaaa aauaucguua agaggcgggg aauuucuuac      360

cuggaugacg ccucggagga uggcggcacc gugucaagcg acuaugggaa ggcagucgag      420

gaaaaccgca aacucuuagc cgagaagaca ccaggacaaa uccagcugga acgauucgag      480

aaauacgguc agcuuagagg cgauuuuacu guggaggaaa auggggagaa gcacagguug      540

auaaacguau ucaguaccuc cgcuuaucgg aaagaagcag agcguauccu gcgcaagcag      600

caagaauuua auucuaagau uacagacgag uucaucgagg auuaccuaau uauccucacc      660

ggaaaaagaa aguauuacca uggcccgggu aacgaaaaga gcaggacuga cuacgggcgg      720

uuucgaacag auggaaccac gcuggacaau auauucggca uucuuaucgg caagugcacu      780

uuuuauaccg aggaauaccg cgccuccaag gcuucauaua cagcccagga guucaaccug      840

uugaacgacc ucaauaaccu gacuguuccc accgaaacaa agaaauuaag ugaggagcag      900

aagaagcuga uuaucgaaua cgcgaaaagc gcaaagaccc ucggggccuc uacgcuucug      960

aaauauaucg cuaagaugau ugaugccucc guggaccaga uaagaggaua cagggucgau     1020

gugaauaaca aaccugagau gcacacuuuu gaaguguauc ggaagaugca aucauuggag     1080

acaaucaagg ucgaagaacu accacgcaaa guucuggacg agcucgcaca uauucugacc     1140

cuuaauacug aaagagaggg uaucgaagag gcuauaaaca gcaagcugaa agauauuuuc     1200

aauagggacc agguguugga gcucguacag uuccguaaga acaauaguuc ucuguuuucc     1260

aaaggcuggc acaacuucag caucaaguua augauggaac ugauccccga gcuauacgaa     1320

acaucggagg aacaaaugac cauucucacg cggcugggga agcagcgauc uaaagagacc     1380

uccaagagaa caaaauacau cgaugagaag gaacuuacug aggaaauuua uaauccugug     1440

gucgccaaau cagugaggca ggcgauaaag aucauuaacg aggccaccaa gaaauacgga     1500

aucuuugaca auaucguuau ugaaauggca cgcgagaaca augaggaaga ugcuaagaaa     1560

gacuauauaa agcggcagaa agccaaccaa gaugagaaga acgcugccau ggaaaaagca     1620

gccuuccagu acaauggcaa gaaggaguug ccagacaaca ucuuucacgg ucauaaagaa     1680

cugacaacua agauuagacu cuggcaccag cagggggaga aaugucugua uaccggcaag     1740

aauaucccca uuagugaccu uauccauaac caauacaaau augagauaga ucacauccug     1800

ccuuugagcc ucuccuucga cgauucucug agcaauaagg uguuaguccu ggcuacagcg     1860

aaccaggaaa agggacagag gaccccauuu caggcacuag acucaaugga ugacgccugg     1920

aguuaccgcg aguucaaauc cuacgugaag gauagcaaac uccugucuaa uaagaaaaag     1980

gacuaucuuu ugacugaaga ggauauuuca aagaucgagg uaaaacaaaa guuuauugaa     2040

cgaaaccugg uugacacgcg guacaguagc cguguggucc ucaaugcucu gcaggauuuc     2100

uauaaauccc accaguuaga cacaaccauc ucugugguca gaggccaguu uacuucgcaa     2160

cugaggcgca agugggggau agagaaaagc cgggaaaccu accaucacca ugccguggau     2220

gcacuuauua ucgccgcuuc cucacaguug agacucugga agaagcacag uaacccgcug     2280

auugccuaua aagagggaca guucguugac ucugaaacag gugagaucgu gagccuaucc     2340

gacgaggaau acaaggagcu gguauuuaaa gcacccuaug aucauuucgu cgacacccuc     2400

aggucaaaga aauucgaaga uagcauacug uuuucuuacc aaguggacuc caaguacaau     2460

cgaaaaauca gugaugcgac uauuuaugcu acacgcaagg ccaaacuuga caaagagaag     2520

aaagaauaca cguauaccuu gggcaagauc aaagauauuu acgcacuggg gacuaagaca     2580

ccuagcaaga ccggauucua uaaauuuuua gaccucuaca agacagauaa aucucaguuc     2640

cugauguauc agaaggaccg gaaaacuugg gaugagguua ucgagaagau aauugaacag     2700

uacagaccau uuaaggagua cgacaaaaac ggcaaggaag uggauuucaa ucccuuugag     2760

aaauauagga ucgguaacgg gccuauccgu aaguacucca aaaagggcaa uggaccagaa     2820

auuaagucac uuaaauauua cgacauccug uugggcaagc acaaaaacau uacccccgac     2880

gggagucgca acacggucgc ccuccugagc cuaaauccgu ggcggaccga uguguauuac     2940

aacucggaga caaagaaaua ugaguuccug ggacucaagu acgcugaccu gugcuuugaa     3000

gaggguggcg ccuacgggau auccgaagua aaguauaaaa agaucagaga gaaagaggga     3060

auuggcaaaa auucugaguu caaauuuacu cuuuacaaga acgauuugau ccugauuaaa     3120

gacaccgaga caaauuguca acaguucuuu agguucugga gccgaacugg uaaggauaac     3180

ccuaagucau ucgaaaaaca caagaucgag uuaaaacccu augaaaaggc aaaauuugag     3240

aagggggagg aacucaaggu gcugggcaaa guuccaccua guuccaauca guuccagaag     3300

aacaugcaaa uagagaaucu uucuaucuac aaagucaaga ccgacauucu gggaaacaaa     3360

cauuuuauca agaaggaagg cgaugagccc aaacuaaagu ucaaaaagug a              3411


<210>  2841
<211>  3420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2841
augagcgacc uggugcucgg ccuugauauc gggauuggau ccgucggugu uggcauacug       60

aacaagguga ccggggagau cauucacaaa aauucuagaa ucuuccccgc cgcucaggca      120

gaaaacaauu uggagaggcg gacaaaccgc caaggacgac guuuaacuag aaggaagaaa      180

caucggcgcg uaagacugaa ucaccuauuu gaagagucag gccucauuac ggacuucacc      240

aaggugagua ucaaccugaa uccuuaccag cuuaggguca aaggucugac agccgaauug      300

agcaacgagg agcucuuuau agcgcugaag aauaugguga agcaucgggg gaucuccuau      360

cuggaugacg cuucugauga cggaaacucg ucaguuggcg auuacgccca gauugugaaa      420

gaaaauagca agcagcucga gacuaaaacc ccaggccaaa uccaguuaga acgcuaucag      480

aaguacgggc agcugcgagg agacuucaca gucgaggagg augguagaaa acacaggcuu      540

auuaacgugu uuccgaccag ugcauaucau gccgaagcau ugcggauccu gcaaacucag      600

caggaguuca auccccagau aacagacgaa uuuauuaacu ccuaccuaga gauccucacc      660

ggcaagcgua aguauuacca cgggccugga aaugaaaaau cucgcacgga uuacggcaag      720

uauacuacca aaaaggacgc ucaaggucag uacauuacac ugaacaacau cuucgggauc      780

cuuauuggaa aaugcacuuu uuauccagag gaguacagag ccgcuaaggc cagcuauacc      840

gcgcaggagu ucaaucuguu gaacgaucuc aauaaccuga caguacccac cgaaacgaaa      900

aaguuauccg aggaacaaaa guaccagaua aucacuuaug uuaaaaauga aaaggcaaug      960

ggcccugcca aacuguuuaa guacauugcu aaacuccuuu caugugacgu ggccgacauc     1020

aagggcuaca ggauagauaa gagugacaaa gcagagauuc acacauucga ggcuuaucgg     1080

aagaugaaaa cccuggaaac uuuggauguc aagaaaaugg cccgagagga acuagacaag     1140

cuggcguacg ugcucacacu gaacaccgag cgcgaaggga uccaggaggc ccuugaucau     1200

gaguuugcag acggaacguu cagccaggaa caaguggaug agcuggucca guucagaaag     1260

gcuaauucuu ccaucuuugg uaaaggcugg cacucauuca gcguuaaguu gaugauggaa     1320

cucauuccag agcuguaugc caccagugag gaacagauga caaucuuaac uaggcugggg     1380

aaacagaaga ccacaucuuc cagcaacaaa acuaaguaca uugacgagaa gcaacuaacc     1440

gaagagauau auaaucccgu gguagcuaaa ucggugcggc aggccaucaa gauugucaac     1500

gcagccauca aaaaguacgg agauuuugac aauaucguga uugaaauggc ucgcgagaca     1560

aacgaggaug acgaaaagaa ggcgauacag aaaauccaga aggcaaauaa ggccgagaaa     1620

gacgcugcca ugagaaaggc agccaaccaa uauaauggca aagcugaacu cccucauucu     1680

guuuuccacg gucacaagca gcuggccacc aaaauuaggc uuuggcauca gcagggggag     1740

cguugcuugu acacuggcaa gacgaucucc auucacgauc ugaucaacaa cccaaaucaa     1800

uuugaaauag accauauccu cccgcuguca auuacauucg augacagucu ugcaaacaag     1860

gugcuggucu acgcgaccgc uaaucaggag aaaggacagc ggacucccua ucaagccuug     1920

gauagcaugg acgaugcaug guccuuucga gagcucaagg ccuucgugag agaaucuaaa     1980

gcucugagca acaagaaaaa ggaguacuua cugaccgaag aggacaucuc aaaguuugau     2040

guaaggaaaa aguucauuga acgcaaucua guugacacac gguaugccag uagagugguc     2100

cucaacgcac ugcaggagca cuuuaggacc cauaaaaucg auacuaaggu guccgucgug     2160

cgcggccagu ucacaagcca gcuucgacgg cacuggggga uagagaaaac gcgugacacc     2220

uaccaccauc acgcuguuga ugccuugauu aucgcggccu cuucacaacu gaaucucugg     2280

aagaagcaga aaaacacucu ggugaguuau agcgaagacc aguuacugga cauugagaca     2340

ggagaacuua ucuccgauga cgaguacaag gagucuguau uuaaagcucc uuaucagcau     2400

uucgucgaua ccuugaaguc gaaagaguuc gaagacagca uacucuuuuc cuaccaagug     2460

gauucaaagu ucaauagaaa aaucagugac gcaacaauuu acgccacuag gaaggcuaag     2520

cuggauaaag aaaagaaaga guauaccuac acgcuaggua agaucaaaga cauuuaugcc     2580

cugggcacca agacaccauc uaagacuggg uuuuacaaau uccucgaucu guauaagaaa     2640

gacaagagcc aguuucuuau guaccagaaa gaucgccgga ccugggacga aguuaucgag     2700

aagauauugg agcaguauag acccuucaag gaaaaagaua agaacggaaa agagguggac     2760

uuuaauccuu ucgaaaagua caggauugag aacggcccaa uccgaaaaua cucccgcaag     2820

gguaaugggc ccgaaaucaa gucacugaaa uauuacgaca acuuacucgg ccgguuuguc     2880

gauauuacac cgagcgaguc uaagaauccu guggcacugc uuucccugaa ccccuggaga     2940

acugacguau auuacaacac cgagacgagg aaauaugagu ucuugggacu caaguacgcg     3000

gaucuguguu uugaaaaagg cgggaguuau ggaaucagca aggugaagua caauaaaauu     3060

cgugaaaagg aggguauagg caaaaacucu gaguucaagu uuacacuaua caaaaaugac     3120

cugauccuca uuaaggauac cgaaacuaac cgccaacaga ucuuccgguu cugguccaga     3180

acagggaaag acaauccaaa gucauuugag aagcacaaac uggaacuuaa gccuuaugag     3240

aaaaccaggu ucgaaaaggg agaggaguug aaaguucugg gcaaggugcc cccaaguagc     3300

aaccgauuac agaagaauau gcaaauugaa aaccucucga ucuacaaagu ccggacugau     3360

gugcugggua aucagcacau aaucaagaac gagggggaca aacccaaacu ugauuuuuga     3420


<210>  2842
<211>  3420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2842
augaacuacu auaauggcuu caucauuacc gagaacgcca uggcuagcaa gcacgaaaau       60

cugggggcac ucacauuugg acuugacaua gguaucgccu ccgugggcug ggcgguccug      120

ggggagaaca gaauuaucga uuugggaguu aggugcuucg acaaagcuga aacugccaag      180

gagggcgaau cuuuaaaucu ggcacggcgc acggcccgac uacuccguag aaggcugcgg      240

cgcagagcuu ggaggcuuac caaacuggca cgguugcuca agcgcgaggg ucugauugcc      300

gauacaaacg uguuucagaa acccuucaau cugaaggacg cuaacgagaa ggggcucuca      360

uuuuggcagu uacgaguaga ugcccuggac agaaaacuua gugcggaaga gugggcaagg      420

gugaucuacc auuuguguaa gcaccgggga uuccauugga uaagccgugc cgaagagaaa      480

gcugccgagg gcgauuccaa gucugaaggc gggaaaguca agcagggacu ggcagguacu      540

aagaaacuaa ugcaggagaa gaauuaucgc accgcugccg aaauggugcu cacagaguuu      600

ccugaagcgc agagaaacaa acaaggcgac uacucgaagg cccugucaag gauccuucug      660

ggggaugaga ugacccaguu guucaaacac cagcgggagc ucggaaaucc acaugcaacu      720

gaaaagcugg aggcugccau uuuaggcaac ggugaccaca aaagcgggcu guuuugggcu      780

cagaauccgg cccucgcagg aaacgaucuu cugaagaugu ugggccacug cacauucgaa      840

aaggccgagu aucgaguucc caaagcuagu uuuaccgcgg aacgccaugu guggcuaacg      900

agacugaaua accucaggau cgucguggac ggcacuaccc ggccucugaa cgaggcagag      960

cgccaccuug cccuguugau gccauaccaa caggcugggg aucucacaua uaagcagcug     1020

agaacugccu uaaccaaagc aggauaccug cccgaauccu ucagguacac aggucuagcc     1080

uaugcuucug gccagaaggc cgacgagaaa gcaaaggacc cugaaagcgc gccacucgua     1140

aagcugcccg cuuggcauga gcuucguaaa accuuggaaa agaaagagcu ggccuccgag     1200

uggcaaaaga uuucaaguga ugcacucgac gggaaaccuc agcugcuuga ucagaucgcc     1260

uggguucuga gcguguacaa ggacgauacg gaagucgagg cugaauugug uaagcucaau     1320

cugccaaacg ccgagaaaau gauagacgca uuacuggaua uucgguuuga caaguucucu     1380

aaucuauccc ucaaagcucu gcgacaaauc gugccgcuua uggagcaggg acagagauau     1440

gaugaagccg uggcgaacau ucccgaguac ggccaccacu cacaguugca ugaagucggu     1500

gccggggagc acaaguaucu gccuccauuc uacaaagaaa gggacaagac uggccgcaug     1560

guuuuuaaug augacaugga caucccccgg aacccugugg uacucagagc ucugaaucaa     1620

gcaaggaagg uggucaacgc cuuaaucaaa gaguauggaa gcccacauga ggugcacauu     1680

gaaauggcuc gcgaucugag ucgaccccuu gacgagcggc gugauguuga aaaguugcag     1740

aaagaguuca gagacaggaa ugaaaaggau aaagcccucu uugcacagga cuucaacaua     1800

gugggcgcgg ucaaggggaa agaguuugag aaguaccagc uguaccgcga acaacaggga     1860

aagugcgccu auucuaucga gccgauggau auucaucggc uauucgaaca ggguuacgug     1920

gagaucgacc acgcucugcc uuauuccagg agcuuugaug acucgaaaaa uaacagggua     1980

cucguucugg ccaaggaaaa ucgagauaaa ggcaaccgca cacccuucga guaccuuggg     2040

ggaaaggaca auucugagcg guggagacag uuuguggcau ucgucgaagg caacaaaucc     2100

uauagguugg cuaagcguuc acgccuguua cggaaggauu uugguaaaga cgaggccaag     2160

ggguucagag aaaggaaccu caaugacacc cgauacauau gucgguuuuu caaaaacuau     2220

guggagcaau accugcagcu ucacgaggau agugacgcaa agcgcugcgu cgugcugagc     2280

ggccagauga cuuccuucuu gagagccagg uggggacucg uuaaagugcg gggcgauucu     2340

gaccgacauc acgcucugga uggggcgauu guagccgcau guagccauuc aauggucaag     2400

agacuaagug acuacucccg caggaaggaa cuggaacagg ugcgugcugg auuuguugau     2460

guggacacag gugagaucgu caauccagcc auguuccaac aggugcagga gcacuuuccu     2520

aaccccuggc cacacuuccg ggaugaagua aaacucagac ugaaugugga cgaucccgcc     2580

cuuuugcaua gcgagaugaa cagguuuggc accuauucuc cugaggcucu gaaggcauua     2640

cgcccacucu ucaucucacg agccccgcaa agacggaaua guggggcggc ucacaaagac     2700

acgauuuacg cccagcccga aaggcugguu gcacagggag gcgucaccca gaaggugccu     2760

cuuacaagcc ugacucuaaa agaucucgac aagcugaucg acccacaucg caacgagaag     2820

uuguauuccg ccauucguau aagacuggaa caacacggug ggaaaggcga uaaggcuuuu     2880

cccgcaucua auccuuuacg gaaaccagac ucggagggaa accccaccgg cccgaucgug     2940

agagggguca caaugguuau ugauaagcuc agcggaaucc cuauccgcgg cgguauugcc     3000

aaaaaugacu ccauggugcg aguggaugua uucacuaagg cggggaaauu ucaucugguc     3060

ccaguguacg uucaccacgc uguggccaag guccuuccca acagagcagu gguugccuau     3120

aaggacgaaa ccgaguggac acugauagau gacggauucg auuuuugcuu cucaauguac     3180

ccuaaugacu ugcuccggau caaacugagg aacgcuaguc aucuaggcua uuacgccucu     3240

uguaaucgca gcaccggugc aauuaaccug uggucccacg auagaaacca acaggugggc     3300

aaggacggga ugaucgaguc aauuggagua aaaacugcuc uuaaugucga aaagcucaac     3360

aucgacgugc ugggcaauag guaccccgcc ccaccugaga aacggcguga uuuggcguga     3420


<210>  2843
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2843
augaagaaca ugaccaaagu gaaugucggc cuggacguug ggaucagcuc cgccggaugg       60

ucugugauua acgcugagac aaaucagaua cucgaaucag guguaagacu uuucaguggc      120

agcgaugcag acgagaacgu gaauaggcgg aaccaacgcc aggggcgacg ucuguuuaga      180

aggcggaagu ugcgcaucca ggaagccaaa aaguacuuag agaaugucuu ccagcugaaa      240

gauauugaac ccaaggacga guggaacccu uaugagauca gagugagggc gcuaaaugaa      300

aagguuucca aagaggaacu cucuauugcu cugcuuaaca ucgugaagca ccggggaaua      360

ucguacgagc uggaggaugu cacugacgaa gagggcacga auacccaauc auuucagagc      420

gguuuggauu auaaccgccg acuccugaaa acaaagacug ugggggaaau ccaguacgac      480

agacugcaga aauauggaca aguaaggggc cagauucaga ccgaggaugg ccucuuaauc      540

aauauuuucc caacaaagaa cuacguugaa gagguggagc ggauccugga aacccagaag      600

aaauaucauc cggagauaga cgaugucuuu auugaaaaga ucauuaguuu cgugacucgu      660

aaacgcgagu acuuuguggg gcccggaaau gaaaagucca gaacagacua cgguaucuau      720

agggagaacg gcgagacccu ugauaacuug uucgaaaucc ugauugggaa agacaaguuu      780

uacccugagg aauaucgggc cgcaggaucu agcuacacgg cccaaauauu caaucuacuc      840

aacgaccuga auaaccuucg aaucccaacu accgaggauc accaucugac aaaagcucag      900

aaggaagaga uuaucacuga guugaagaau accucccgcc ucucaggcgg uaugcugaaa      960

uuaauaagua aggucacaaa agcacccaag gaagagauua gcggguauag aaaagacgaa     1020

aaggauaagc cugaccugca cucucucgcc uacuauagga aacagaagaa caugcuugag     1080

aaagagggaa uccaaaucgc ugauuuuucc gaagaacugu uggacgaacu agccaccaug     1140

augacgcuga auacugagcc aggcgaaauu cggaagcagc ucaacaaaau cacaaaucag     1200

uacgauauuc ugacccccga gguuauagag gugcuuguaa agaaccauac ugcguucgug     1260

gcaacaucaa aucacaagug gcaccgcuuu agccugaaau ugaugaagga guucaucccu     1320

gagcuccugg aaaccaguaa agagcagucu acgauuuuau gcgaaagagg cuucauccaa     1380

aacagggccu ccuaccugga cgagaaucau cacuuuaacu augcugagcu acgugaaaau     1440

cucuacaacc cagucgugcg gaagucuacc ugugagaugc ugaaaguuuu cacagccauc     1500

caggaaaagu auccgaacau ucagaaugug auagucgagc uuccccgaga aacuaacgag     1560

gaugagcaga agagaaaaau caagaaaauu aaugacaaca augcaaagag gaaaaaggaa     1620

aucuugaaag aggcucaagc ccagucgggg cugucugaag aggcgcucga ggaaaagcug     1680

cuuaagacca aaggacugua cuugaagcuc augcuguggu augagcagga agguaaaugc     1740

guguacuccg gccaggagau uccuuuauca gaucugcuac aaaacaaagg gggauaugaa     1800

guagaccaua ucauaccaau caguauuagc uuugacgauc gccucuguaa uaagguucug     1860

gugcggcagc agcuuaacuc ccagaagucu aaucaaacac ccuucgagua caugacuacc     1920

cacacagacg gccacuugag cuuucaugag cugaaggaac ucgucgguaa aaacaagaga     1980

uucucaaaag agaagaggga acuguuacug gccgagaucu acaguaauga uauugaaacc     2040

cgcaaacgau uuaucaaccg gaaucuugug gacacuuccu auacggcacg uauagucuug     2100

aacgcucucc agcaguacaa gaaggagaau caacugagca ugaacguguc uguugugaga     2160

ggggccauga caaacauucu aaggaaaaag guaaaacugg auaagacccg cgagacuuuc     2220

caccaucacg cuaucgacgc cucacucaug gcagucauuc cuaauaucga auuuuucaaa     2280

gagcggaagg aauggcugcc aaagagugcc aacgaggaag ugguugaggu ggaggucagc     2340

gaagaggaaa ccaugcccga gaaagaguuu aaggugaaaa ugagacuucc ggaauccuuc     2400

auacaggaug uacagaucuu gcagaggaau agcuuugcua aggaggaagu gucgggcccu     2460

caaacacgag agcgcaaccu gaaaauuaag uucagccauc agguugacca gaaaaugaau     2520

cggaaggucg cggaugacac caucuaugga acuagacagg cacaauuagg caaggauaaa     2580

aaggaagaga cauacgugau ugggaaaauc aaggacauau auaacuacga ugaguaugug     2640

agguucaaaa agcguuacga caagaauaaa gaauccuuuc ucauguacca ccacgacccc     2700

cagacguucg agaagcugga agagauucuu gcccaguauc cagaauacau cgaggaugag     2760

aaaggaaaga aaaagaaggu cucaccuuuu gcucuguauc gcgaagagaa cggucccguu     2820

accaaauacg ccaagaaaaa uaacggccca gugaucaagc aguugaaaua uuacgacagu     2880

aagcucaauc ggaagauuga uaucucucau aaauauagca cuaagaacag acaaguggua     2940

cugcaguccc uaaaacccua caggacagac gucuaccgaa aucaggauac cggggaauau     3000

acacuggugg gaauugacca aaacgauggc aaguucguua auggucacua cgggauaccu     3060

aaaucacggu augaggaaau caaggcaaac aacaagcuca gcgagcauga cgaguuugug     3120

uucucucugu acaaaaauuc cauggucaag gugauugaug aaaacggcga gaguaucguu     3180

gugcgcuauc uuagcagauc uaugccaaau aggaaaaaca augccgaaau uaagccggua     3240

gagaaauacc aguuugagga cggagaaguc gugcccgugu auggcaagug ccggaaacag     3300

augcagacug ucuucguuaa gaaggggugg accuugcuga aaggaaacac ggauaucuua     3360

gguaauaccu acuuugugga gaaggaaucc gagaacccua aaaagaugau agacgauaaa     3420

uga                                                                   3423


<210>  2844
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2844
augagagaga acggcagcga cgaaaggcgg cgcaauaugg augagaagau ggacuaccga       60

aucgggcugg auauuggaau agccuccgug gguugggcug uccuccagaa caauucugac      120

gaugaacccg uucguaucgu ggaccuuggc guaagaauuu ucgauaccgc agagaucccu      180

aaaacagggg aaucacuggc cggaccaagg cgggcggcuc gcacuacgag aaggcgguug      240

cgccgaagaa agcacagguu agaccggauu aaauggcugu uugagaacca aggccuaauc      300

aauauagaug acuuccucaa gcguuauaac auggccgguc ugccggaugu guaccagcuu      360

cgcuaugagg cacuggacag aaaauugacc gaugaagaac ucgcccaggu ccugcugcau      420

aucgcuaagc acaggggguu ucggaguaca cgaaaggcag aaacugccgc uaaagagaau      480

ggagccgugc ucaaggcgac cgacgagaac cagaaacgca ugcaagaaaa gggcuacaga      540

acaguuggcg agaugauuua uuuagacgaa gcauucagga ccgggugcag cugguccgag      600

aaaggauaca uccugacucc ccggaauaag gccgaaaacu aucagcauac aaugcuucgc      660

gcuauguugg uggaggaggu caaggaaauc uuuucuucgc agagaaggcu ggguaaugag      720

aaagccaccg aagaacuaga agagaaguac cucgagauua ugacgucaca gcguagcuuc      780

gaucugggcc cugggaugca accagacgga aaacccaguc cuuacgcaau ggaaggcuuu      840

uccgaucggg uggguaagug uacuuuccuu ggggaccagg gagagcugcg aggcgcuaaa      900

ggcaccuaua cagccgaaua cuuuguagcg uugcagaaga uaaaccacac uaaacucguu      960

aaucaggaug gggagaccag aaacuucaca gaagaggaga ggcgcgcccu gaccuuacug     1020

cucuuuacgc aaaaggaagu gaaguaugca gcuguccgga aaaagcuggg acuuccagag     1080

gacauuuugu ucuacaaucu aaacuauaaa aaggccgcua cuaaagaaga acagcagaag     1140

gagaaccaaa auacagaaaa ggccaaauuu aucgguaugc ccuacuauca ugauuacaag     1200

aaaugccugg aggaaagagu gaaguaccuc accgagaacg aagugaggga ccuguucgau     1260

gagauuggca ugauccuuac uuguuauaaa aaugacgauu cucgcacaga gcgacuggca     1320

aaguuggggc ucgucccuau cgaaauggag ggacuguuag ccuacacccc aacgaaguuc     1380

cagcaccuga gcaugaaagc uaugcggaac auuauaccgu uucuagaaaa gggcaugacc     1440

uaugacaaag cgugcgagga agcagguuac gacuucaagg ccgauuccaa agggacaaag     1500

cagaagcucc ugacuggcga gaauguuaac cagaccauca augagauuac aaaccccgug     1560

guaaaacguu cagugaguca aacugucaag gugaucaaug cuauaauuag aaccuaugga     1620

agcccucagg ccaucaacau cgaacuugca agggagaugu cuaaaacauu ugaggagcgc     1680

cggaagauua aaggcgacau ggaaaagaga cagaaaaaua acgaggaugu uaagaagcag     1740

auccaagagu uggggaaacu guccccaacc ggacaggaca uucucaagua caggcugugg     1800

caggaacagc aagguauaug uauguauuca ggcaaaacua ucccccuuga ggaacuguuc     1860

aagccugggu acgauauuga ccacaucuug ccauacagca ucacguuuga ugacaguuuc     1920

cgaaauaaag ugcucgucac aucucaggag aaccgccaga agggaaaucg gacccccuau     1980

gaguacaugg gcaacgauga acagagaugg aacgaguuug aaacuagggu gaagaccaca     2040

auucgugacu auaaaaagca acagaaacug uuaaagaaac auuucuccga ggaggagcgc     2100

agcgaguuua aggaacggaa ucugaccgau acuaaguaca uaacaacggu aaucuauaac     2160

augauuagac agaaucuaga gauggccccg cucaacaggc cugagaaaaa gaaacaaguu     2220

cgagcuguga auggugccau caccgcauac cugcggaagc gcugggggcu uccccagaaa     2280

aacagagaga cugacacaca ccaugcgaug gaugcugucg ugauugccug cuguaccgac     2340

ggcaugaucc agaagauauc gagguauaca aagguccggg aacgaugcua cucuaaagga     2400

acugaguucg uggacgcaga gaccggcgaa aucuuuagac cagaggauua cucccgcgcc     2460

gaaugggacg agauuuucgg gguucacauc ccuaagcccu gggaaacguu uagggcugag     2520

uuggaugugc guaugggaga cgauccaaaa gguuuccugg acacccacuc agauguagcc     2580

cucgagcugg acuaucccga auacauuuau gagaauuuac ggccuaucuu cgucaguaga     2640

augccaaacc auaaggugac aggcgcagcu cacgccgaua cuauaaggag cccgcgccau     2700

uuuaaagacg aagggauugu ucugaccaag acagcgcuua cugauuugaa acucgacaag     2760

gauggagaga ucgacggcua cuauaauccc caguccgacc ugcuacugua cgaggcccuc     2820

aagaaacaac ugcuuuugua ugguaacgau gcuaagaaag cauucgccca ggacuuucac     2880

aagccuaaag cugaugggac cgaaggccca gugguccgaa aggugaagau ucagaaaaag     2940

cagacgaugg gaguauucgu ggacucuggc aaugggaucg ccgagaacgg aggcaugguu     3000

agaauagaug ucuuucgggu gaaugguaaa uacuacuucg ugcccgucua uacagcagac     3060

guugugaaga aagugcugcc uaacagggcg agcaccgccc auaagccaua cggggaaugg     3120

aagguaaugg aggauaaaga cuuuuuauuc ucacucuaua gucgcgaucu gauccacauu     3180

aaguccaaaa aggacauccc cauuaaaaug gucaauggag gcauggaagg uaucaaggag     3240

acuuacgcuu auuacauagg cgccgauauu agcgcagcua acauccaagg gaucgcccac     3300

gacucucguu auaaguuuag aggacuuggc auucagucac uggacguguu ggagaaaugu     3360

cagaucgaug uucucgggca ugugaguguc gugcggagcg aaaagaggau gggauucucc     3420

uga                                                                   3423


<210>  2845
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2845
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

gagaaaaauu ccgggaaaau cgugcacgcu ucuucaagaa uuuuccccgc agccaccgcg      120

gauaacaaug uagaaaggcg gaagaaccgc caggcucgac guuugaauag aaggaaaaag      180

caucggggag ugcgcuuuca agacuuauuc gaggauuacg gccugcuaac agacuuuagu      240

aaagucagca ucaaccucaa uccuuauaga cugagggugg auggucuuaa ccagcagcug      300

acuaaugaag aguuguucau ugcccucaag aacaucguua agcggcgcgg gauauccuac      360

cuggacgaug caucugaaga cggaggcacg gugucgucag auuauggcaa agccgucgag      420

gagaaucgaa agcugcucgc ugaaaagacc ccagggcaga uccaauuaga gagauuugaa      480

aaguacggac agcugagggg ugacuucaca gugguagaga acggcgagaa augccggcuu      540

auuaauguuu uuagcacaag ugcauauaag aaggaagccg agcguaucuu gcgcaaacag      600

caggauuuca acaaucaaau uaccgacgaa uuuaucgagg auuaccugaa gauacuaaca      660

gggaaaagaa aguauuacca cggaccgggc aacgaaaaau ccaggaccga cuacggucgg      720

uauacuacac auaaggacuc uaaaggggag uacgugaccc ucgauaacau uuucggaguc      780

cugaucggca aguguacguu uuaucccaau gaguaccgag cuagcaaggc cuccuauacu      840

gcgcaggagu ucaaccuucu gaaugacuug aacaaucuca ccgugccuac agagacuaaa      900

aagcugucag aagaacagaa aaagaccauu aucgaauacg caaaaagugc caagacauua      960

ggcgcuagca cccugcucaa guauaucgcc aaaaugauug auacgucugu ggaccagaua     1020

cgcggguaca gagucgaugu uaacaauaag ccagagaucc acacuuuuga gguguacagg     1080

aaaaugcaau cccuugaaac aauuucagua ggagagcuga gccggaacgu guuggacgaa     1140

cuagcacaua uccugacccu caauacugag cgcgaaggua uagaggaggc uauuaacaca     1200

aagcugaaag auaguuucuc ucaggaccag guccuugaac uggugcaauu cagaaagaau     1260

aacuccagcu uguuuucgaa gggcuggcac aauuucucuc ucaaacugau gauggaguua     1320

auccccgaac uguaugagac cuccgaggaa cagaugacga uccuaaccag gcucgggaag     1380

cagaaaucaa aggagacaag uaaacguacu aaguacauug augaaaagga gcugaccgaa     1440

gagaucuaua acccuguugu ggccaaaagc guccggcagg cgauuaagau aaucaaugag     1500

gccacuaaaa agcacggaau uuuugacaac aucgugaucg aaauggcacg agagaacaau     1560

gaagaggaug cuaaaaagga cuacauuaaa agacaaaagg ccaaccagga cgaaaaguau     1620

gcugccaugg agaaagcagc cuuccaguac aauggcaaga aagagcuucc agauaacaua     1680

uuucaugguc acaaggaauu ggcuacuaaa aucaggcugu ggcaucagca aggggagaag     1740

ugccucuaua ccggcaaguc cauucccauc ucugaccuga uucacaauca guacaaauac     1800

gaaaucgauc acauacuucc ucugagcuug ucauucgacg auagucucuc caacaaggua     1860

cugguuuuag cgacagcaaa ucaggagaaa ggacagcgca ccccauuuca agcccuggac     1920

agcauggaug acgcuugguc uuaucgggag uucaagucau acgugaaaga aaguaagcua     1980

cucggcaaca agaaaaagga guaucugcuu acugaagagg auaucagcaa aauugaaguc     2040

aagcagaaau uuaucgagag aaauuuggug gacacgaggu acuccucucg cgucgugcug     2100

aacgcccucc aggauuucua uaagaaucau aacuuugaca caaccauuuc gguugugcga     2160

gggcaguuca cuagccaacu gcggcguaag uggggauuag agaaauccag agaaaccuac     2220

caccaucacg caguagaugc ccugaucaua gcugccucaa gucagcuuag guuguggaag     2280

aaacagaaua acccgcucau ugcauauaag gagggucaau uugucgacuc ucagacaggc     2340

gaaaucauua gccugaccga cgaugaguac aaagagcuag uguucaaggc gcccuacgac     2400

cauuucguug auacucuguc cucaaaaaca uuugaagaca gcauccucuu cucuuaucag     2460

guggauucca aguuuaaucg caagauaagu gacgcuacga ucuacgccac ccggaaagca     2520

aagcuggaua aagagaagaa agaauauacu uacacacuug ggaagauuaa ggacaucuau     2580

agcuugggaa ccaaaacacc uucuaagacu ggcuucuaca aauuucugga uuuauauaac     2640

aaggacaaau cccaguuccu cauguuucaa aaggauagaa agaccuggga cgaggucauu     2700

gagaaaauca uggaacagua caggccauuc aaggaauacg auaaagccgg uaagcuggug     2760

gacuuuaacc ccuucgagaa auaucgacag gagaaugggc cuauacgcaa guacucaaag     2820

aaaggcaacg gaccagaaau uaagagucuu aaauauuacg acauccuguu gggcaagcac     2880

aaaaauauca cgcccgaggg gagccggaac accguagcuc uccugucgcu aaauccgugg     2940

agaacagaug uguauuacaa cauggaaacu aagaaguaug aguuucuggg acucaaauac     3000

gccgaccugc cuuucgaaga ggguggcgca uacgggauuu ccaccgagac auauaaugaa     3060

cuuagggaga aggaaggaau cggcaaaaac ucugaguuua aguucacuuu guacaaaaau     3120

gaucugauuu uaauaaagga caccgagacg aacugucagc aauucuuucg uuucuggagc     3180

cgcacaggua aagauaaucc caagucauuu gaaaagcaca aaaucgagcu caagccauau     3240

gaaaaagcua aguucgagaa aggggaggaa cuggagguuc uuggcaaggu cccucccagu     3300

uccaaccagu uucagaagaa uaugcagauu gaaaaccugu cuaucuacaa agugaagacc     3360

gacgugcuag gaaauaaaca uuucauuaag aaagagggcg auaagccaaa gcucaaauuu     3420

uga                                                                   3423


<210>  2846
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2846
augaacggcc uggugcucgg gcuugacauc ggaauugcca gcgucggugu uggcauacug       60

aagaaagaua ucggggagau uauccacacc aauuccagau uguucucugc ugcaacagcc      120

gacucaaaca uugaaaggcg gggacaucgc ggcgguaagc gauuaacucg uaggaaaaag      180

cacagaagua uccggcugca ugaucuauuu gaggacuucg ggcuccugac ggauuuuagc      240

aaagugucca uaaaucuuaa ccccuaccag cugcgcguac aaggauugga caaucagcuc      300

accaacgaag aacuguucau cgcgcugaag aauauuguga agagaagggg caucucuuau      360

cucgaugacg cuucggaaga uggcgggaca gucucaagcg acuacggaaa agccguggag      420

gagaaccgga aguuacuggc agaacagacu ccuggucaga uucaacuuga ucgcuuugag      480

aaauauggcc agguucgagg ggacuucaau guggucgaaa acggagagaa gagaagguug      540

aucaaugugu uuaccacaag ugccuacucc aaagaggcug aacggauacu gcguaagcag      600

caggaguuca acaagaaaau uaccgaugaa uuuaucgagg acuaucuaac uauucucaca      660

ggcaagcgca aauacuauca cgguccaggg aaugaaaagu cuagaaccga cuacggaagg      720

uacacgacua aaaaggaucc ggagggcaaa uauaucaccc uggacaacau cuucggcauu      780

cuuauaggga agugcacauu uuaccccgau gaguaucggg caagcaaggc cuccuacacu      840

gcucaagagu ucaaucuguu gaacgaccuc aacaaucuga ccguaccuac agagaccaaa      900

aaguuaucag aagaacagaa aaagacgauc auuaaauaug ccaagacugc gaagacacug      960

ggagcaagua cccuccuuaa auacaucgcc aagcugauag gugcuagcgu ugaucagauu     1020

cauggcuauc gaaucgaccc aaacaaaaag cccgaaaugc acacuuuuga gacauaccgc     1080

aaaaugcagu cuuuggagac caucuccgug gaggagcuac cuagaaaggu ccuggaugaa     1140

cucgcccaca uucugacgcu uaauaccgag agggaaggga ucgaggaggc aauuaacgcu     1200

acacugaagg acacuuucuc acaagaucag guguuggaac ucgugcaguu ccggaaaaau     1260

aacagcaguc uguuuucuaa gggauggcau uccuucagcu uaaaacugau gauggagcua     1320

auaccagaac ucuacgagac cucggaggaa caaaugacaa uccugacucg ccuuggcaag     1380

cagaaaucua aggagaccuc caagagaaca aaauauauug acgaaaagga guugaccgaa     1440

gagaucuaca aucccgucgu ugccaaauca gugaggcagg cgaucaagau uauaaacgag     1500

gccacuaaaa aguaugguau cuuugauaau auuguaaucg aaauggcacg ugagaacaau     1560

gaagaggacg cuaaaaagga auacauuaag cggcagaaag ccaaccugga cgagaagaau     1620

gcugccaugg agaaagcagc cuuccaauau aacgggaaga aagaacuccc ugauaaugug     1680

uuucacggcc auaaggagcu ggcuacgaag auccgacuuu ggcaccagca gggagaaaaa     1740

ugucuguaca caggcaagaa cauaccaauc agugacuuga uucagaacca auauaaauac     1800

gagaucgauc acauucuccc gcugagcuua uccuucgacg auucucugag caauaagguc     1860

cuagugcucg cgaccgcaaa ccaggaaaaa gggcagagaa cucccuuuca ggcccuggac     1920

ucaauggaug acgcuuggag uuacagggag uucaaguccu auguuaagga uagcaaacuu     1980

uugggaaaua agaaaaagga guaccugcuc accgaagagg acaucucuaa aauagaagug     2040

aagcaaaagu uuauugagcg caaccugguc gauacacggu auucaaguag agugguauua     2100

aaugcccugc aggacuucua caaagagcau caguuugaua ccacuaucag cguugugagg     2160

ggucaguuca caucccaacu ucgccgaaag uggggcuugg aaaaaucucg ggagacguau     2220

caccaucacg cagucgacgc ccucauuauc gcugccucga gccagcugcg ucuauggaag     2280

aaacagaaca auccucugau auccuacacc gaagggcaau uuguggacca ggucacugga     2340

gagaucauuu cacucaguga ugacgaauau aaggagcugg uguucaaagc accauacgau     2400

cauuucguug acacacuuaa gucuaagaaa uuugaggaua gcaucuuguu cuccuaccag     2460

guggacucaa aguauaacag aaaaauuagc gaugcgacca ucuacgcuac aaggaaggcc     2520

aaacuggaca aggaaaauaa ggaguauacu uacaccuuag gcaaaauaaa ggauauuuau     2580

gcacucggua cgaaaucucc cuccaagacc ggguuuuaca aauuccugga ccuuuauaac     2640

aaggauaaga gucaguuucu gauguuccaa aaagaccgca agacauggga ugaaguaauc     2700

gagaaaauca uugaacagua ccggccuuuu aaggaguacg acgagaaugg caaagaaguc     2760

gacuucaacc cauuugagaa guauagaauc gaaaauggac ccauuaggaa guacagcaaa     2820

aagggcaacg ggccggagau aaaaucuuug aaguauuacg auaaucuccu gggaaaauuc     2880

guggacauca cuccuuccga aucaaagaac cccguugccc uacugagucu caacccaugg     2940

cgaaccgaug uguauuacaa uacagagacu agcaaguaug aguuucuggg ucuuaaauac     3000

gcugacuugu gcuucgaaaa gggcggggcc uacggaauuu cggaggucaa auauaacaag     3060

auccgcgaaa aagagggcau ugguaaggag uccgaauuua aauucacccu guacaagaau     3120

gauuuaaucc ucauaaagga cacggagaca aacugucagc agaucuuccg guuuuggucu     3180

agaaccggga aagauaaucc uaagagcuuc gaaaaacaca agauugagcu gaaacccuau     3240

gaaaaggcaa gguuugagaa gggcgaggaa cuugaggugc ugggaaaagu accacccuca     3300

aguaaccaac uacagaagaa uaugcagauc gaaaaccucu ccauuuacaa agugaagacu     3360

gacguucugg gcaauaaaca cuucaucaag aaggaggggg aggaaccuaa auugaaguuu     3420

uga                                                                   3423


<210>  2847
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2847
auggaggaaa acuuccagua cagaaucggc cuggacauug ggauaaccag cgugggaugg       60

gccguccucc aaaauaacuc ccaggaugag cccguuagga ucacagaccu uggugugcgg      120

auuuuugaug uagcugaaaa uccuaagaac ggcgacgcac uggccgcgcc acgccgagau      180

gcucguacua cgagaaggcg guugcgcaga aggcggcacc gcuuagagcg aaucaaauuc      240

cugcuacagg aaaaugggcu cauugagaug gacucuuuua uggagagaua uuacaaggga      300

aaccugccgg auguguauca gcuuagguac gaaggccugg accggaaauu gaaggaugag      360

gaacucgccc aaguccugau ccauauagca aaacaccgug guuuccgcuc aaccagaaag      420

gccgagacaa aggggaaaga gggaggcgcu gugcugaagg caacuaccga aaaucagaaa      480

aucaugcagg agaagggcua uaggacaguu ggggaaaugc ucuacuuaga cgaggccuuu      540

cauaccgaau gccuguggaa cgagaaagga uaugugcuua cuccccggaa ucgaccugau      600

gacuacaagc acacaauuuu gcgcaguaug cuggucgagg aagugcaugc uaucuucgcc      660

gcgcagagag cacuagguaa cgagaaggcc accgaggagc ucgaaaaugc uuacguagag      720

auuaugacga gccaaagguc cuuugauaug ggcccagggc ugcagcccga cggaaaaccu      780

ucuccauaug ccauggaggg cuucggugac cggguuggga aguguacuuu ugaaaaagau      840

gaguaccgcg cacccaaggc uaccuauaca gccgaacuuu ucguggcgcu gcagaaaauc      900

aaccacacua aguugauaga cgaguuugga accggcagau ucuuuacaga agaggagagg      960

aaaaccauua ucgaucuccu guuaucgggc aaggaacuga aguacgggac gauucguaaa     1020

aagcucaaua ucgacccuuc acuuaaauuc aacagccuga auuauagugc caagaaagag     1080

ggagaaacug aggaggaacg gguccgagau acagagaagg caaaguucgc uuccauguuu     1140

uggaccuacg aauauucuaa augcuugaag gacagaaacg aggaaaugac ugugggucag     1200

aaagccgauc uacuggacag gaucggcgag auucucacag cuuacaagaa cgaugacagc     1260

cgcuccucac ggcuggagga ucuugggcug aguagagaag agauagacgg auugcucgaa     1320

cugagcccag ccaaauacca aagggugucu uuaaaggcaa ugcgcaagau gcagccguau     1380

cuggaggacg gccuaaucua cgauaaagcc ugugaagcug cggguuauga cuuccgagca     1440

cucaaugaug ggaacaagaa acaucugcuu aagggcgagg agauuaaugc caucgucaac     1500

gacauaacca aucccguugu gaaacggucc guaucacaga cgauuaaggu gaucaacgcu     1560

aucauucaaa aguacggaag cccucaggcc gucaauaucg aauuggcacg ugagaugagu     1620

aaaaacuuuc aggauagaac caaucuggaa aaggagauga aaaagaggca gcaagaaaac     1680

gagcgcgcca aacagcagau uauagagcuc ggcaagcaga auccaacagg gcaagacauc     1740

cugaaauauc ggcuuuggaa cgaucaggga gguuacugcc uguauucugg caagaagauu     1800

cccuuggaag aacucuucgg gggaggcuac gacaucgauc acauccugcc uuacuccauu     1860

acuuuugacg auagcuauag aaauaaagug uuaguuaccg cucaggaaaa caggcagaag     1920

gguaaucgaa caccauacga guauuucggg gccgacgaga aacacuggga agauuacgag     1980

gcacgcgugc ggcugcuagu cagagacuau aagaaacaac agaagcuccu gaagaaaaac     2040

uuuacugaag aggaaaggaa ggaguucaaa gagcguaacc uuaaugacac caaguacaua     2100

acacgcgugg uauauaacau gauccggcag aauuuggaac uggagcccuu uaaccauccg     2160

gagaaaaaga agcagguuug ggcggugaau ggcgcuguca ccucguaccu cagaaaaagg     2220

uggggacuga ugcaaaagga ucgaucuacu gaccggcacc acgccaugga ugcagugguc     2280

auugccuguu gcacggacgg caugauccau aaaauuuccc gcuacaugca ggggagagaa     2340

uuagcuuauu caaggaacuu caaguuuccu gaugaagaga caggagagau ccugaaucgg     2400

gacaacuuca cccgagaaca gugggaugag aaauuuggug ugaagguucc ccuuccaugg     2460

aauaguuuca gagacgaauu ggauauacgc cuccugaacg aggacccuaa gaauuuccua     2520

cugacucacg ccgaugugca aagggaacuc gacuaccccg gcuggaugua uggggaggag     2580

gaaagcccaa ucgaggaagg acguuacauu aacuauaucc ggccccuguu uguauccaga     2640

augccuaauc auaaagucac cggcucugca cacgaugcua caauuaggag cgcccgcgac     2700

uacgagaccc gagguguggu uaucacuaag gugccacuua cagacuugaa acugaacaag     2760

gauaaugaga uagaagggua uuacgacaaa gauucagaca gauuacucua ccaggcgcug     2820

guccggcagc uucuguugca uggcaacgau ggaaagaaag ccuucgcuga ggacuuucac     2880

aagccgaagg cagauggcac ggaagggccc gugguaagga aagugaagau ugagaaaaag     2940

cagaccagug gaguuauggu ccgcggcggu acugggaucg ccgcuaacgg agaaauggug     3000

cguauugacg uguucagaga gaauggcaaa uauuacuuug ucccuguuua uacagccgau     3060

guggugcgga agguacuccc aaacagggca gcgaugaaug guaagaucgc cuccgacugg     3120

aaaaccaugg aggauaacaa uuucgucuuu agccuguacu cucgcgaccu aauacacgug     3180

aagcgaaaga aagcuauccc cacaauucug ucaaguggcg ccacuuucga acaagaggaa     3240

uuuuaugcau acuucaccgg ggcugacauc agcacgguuu ccauugccgg aaucucuaac     3300

gauaauucgu uuaaauucag aggccucggg auacagggac uggagauuuu ugaaaagugu     3360

cagguggaca uccuuggcaa caucagcguc gugcggcaug agaauaggca ggguuuccac     3420

uga                                                                   3423


<210>  2848
<211>  3426
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2848
auguacagca ucggccugga cauugggaua gccuccgugg gauauucugu caucgagacc       60

aacucaggua gaauuguuga acucggcgcu aggaucuucg aggcacggaa uagugauaac      120

aauaaggacc gccgagauuu ucguggggga agaaggcuua uucggcgcag aaaaacaagg      180

cuggacgaug ccaagcggua cuuggacgau cagggcuuca aaaacgacaa gagcuuaauc      240

aaugugugcc ccuauucccu gcgcguaaaa ggucuaacug aggaacucuc uaagucggaa      300

auacgaaagg ugauccugca cauucuuaaa aagagagggg ucucauaccu gagcagugag      360

gauuuggaca cguccucugu ggauaacaaa gacagcuuua agcaagcggu uaccaaaaau      420

cucaggcagc uggaugacaa gacaccugga cagauccagu augagcggcu gcaagaaucc      480

ggccgugugc gcacuggcau uaaccagaau gggcaguacc agcucaacgu cuucgacgug      540

gcuucauaug ccaaggaguu aaaacugauc cuugaaaccc aacagcagua ccaucaggag      600

auagaugacc acuuuuugga uuucuuuaca aaggacggau acagagagga ugcaggucug      660

auuuauagga aacggccaua cuaugacggc ccggggaaug ccgcucaaaa caguagcuac      720

ggacgauggg caaauuauca gguagauggc aagcccgccg aaaacaucuu cgaccagcua      780

auugguaaag auuuuaccgg gaagauccgc gcuucuaauc ucggaccuuc cgcccaaaaa      840

uucaaccugc uuaacgaccu gaauaacuug agaaucacua caagggagga agagaagcuc      900

accuuugaag agaagcagga gauucuggaa guuuuagaga augaucggcc aaaaacguuc      960

ggcccccgca acauagcgaa gaugcugaaa guggacaugg acgauaucaa gggcuggaga     1020

cucaauucaa gcgacaaacc ugaaauucau acuaugaagg aguacaacga uaugaggcuu     1080

guccuggaag cagggggauu ugacaucaag gccauagauu uggaccacau ugaggagauc     1140

gcuagucuaa ucacccugaa uacagaacgu gaggccuucg aacucucucu guccaacgag     1200

auugauaaua accuuaaaca guuccuggug gaaaauuuua acgcauugcg gaaguguaaa     1260

ccaaauaaca gcuggcauuc guucucuaag gagaaucucc agcgacugau cacugaguua     1320

gaacacaccu ccgacgagca aaacacaauu cuggaaagac uaggucucaa auuugaucug     1380

aggucaaagu auagugaccg cgauacccuu cccauacacg agguguugga ggaaaucuac     1440

aacccuacgg ucagcaaguc cguucggcag acuuucagag ugcugaaugc ucucgugaaa     1500

aaguacggca aagagaacau ugacuaugua acaaucgaaa ugccaaggga uaagaaugag     1560

gacgaccaga aaaagaccau ccgcgaaauu cagaagguca acaaaaaucg aaaggauaaa     1620

ucuaaggagu acuuucugaa aaagagcggg uggucagagg aaaaauucga ggaaaaacuu     1680

cggacaaguc guagauuugc cuccaagcug uauuacuucu augagcaaga cggauugugc     1740

gcguacacug gccagcagau acagccggaa gaucuccuga ccgagagggu ggagguugac     1800

cauaucauuc ccuuaagcgu gucucuggau gacucaauca acaauaaagu ccuagugacg     1860

agugaagcca accaaaagaa aggucagcgc agcccuuaug augcauuuau guccggggac     1920

uucucuaagu accggaccua cgaugaguau aaggcuuggg uacucucgaa uaaguacaag     1980

aaauauaaga aagcccugcu uuuggaagag agaaacauuu uugacccaga acuggcuaag     2040

aaauucaggc agcgaaaucu caacgauaca cgcuacgcca gccggaucgu ucugaaugca     2100

gugcaguccu uuuucuauaa gacugacacc aagacaagag ucgugaacgg cgccuucacu     2160

cacaccuuaa ggaaaaagug gaaucuggcu gauaaagacc gugagacaca ucaccaccau     2220

gcgauagacg caacccuugu cgccgugacu cccuuuguua aggcugagcg cuacgaauau     2280

uacaaagauu cagagggaaa ggccaguaug guggacacgg uaacaggcac caucauuccu     2340

uacucugaau auaagaaaaa gaacuuguac gaucggagca acuaucuccu gauguucggg     2400

aaugagucca cuaucaacca agagaaagaa gacaagucag cagaguuuau uagaaaucua     2460

aucccagcca aaauauaccc cgaaauuaag uucagccacc aggucgauag gaaaucuaac     2520

cgacggcugu ccgacgcuac caucuauagu acacgcagcu acaccgugga gaagggaaga     2580

gguaagaaua aaagggaagu uauugagcgg gauaucauag gcaagaucaa agacauuuau     2640

acugccgaug gguacgcaga guacgugaag uauaaagacu cucuccuggu caaggaaauc     2700

gaugagaaga cauuugaaaa acuugaggcg auuaucgaag aguucuccga cacgguggag     2760

guaaccgacc agcaaggaaa ggugaaaaag guuucaaaaa guccguuuuu gcaguacugu     2820

gaaaaguaug augucccugg cgugacuaag uacagcaaaa agaacaaugg ucccgugaua     2880

cgaucgcuga aauauagaua cacaaaguua cgcucccaua uugacaucuc uccuaaaaac     2940

agcaagaaua ccgucgcucu ccugacacuu gccccaugga ggacugaugu uuauuacuca     3000

caggagaccg caaguuacca ccuguugggg cucaaguaua ucgaccuguc cuuccgugau     3060

ggcggauacg gcgugucucg gaaaagauau gaagagauua agaaaaacga aaggaucccc     3120

gaggacgggg aguuuaaguu cagccuauac aaaaaggauu auauugaaau agaguuugac     3180

aagaaaaucc ugccaggacu cuaccagucc cgcgaugugu cagcccgaaa uaaguucagc     3240

augaaaccca uugacaaggc ucaauggacg aguaaagaag auguacugau guuugguaac     3300

gucaaggccu cuggcguguu cgagaaaacc cuuaagaaga auuauguuuu gagaaaauac     3360

cauguggacg uccuggggaa caaguucgag gugaaagaug aaaucuuaaa ggacauucuc     3420

gaguga                                                                3426


<210>  2849
<211>  3429
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2849
auguacagcg ugggccugga caucgggauu gccuccugcg gauauucuau aaucaacgau       60

gucaauggua acauuguuga gcucggcacc cuucuguuca agucaagaaa uagugacaac      120

aaugaagaga ggcggacaaa ccgcgggagc cgacguuuga ucaauagaag gcggacucgc      180

uuagaugacg gaaagaagcu gcuagcugaa aaaggcuuua agcaggauuc caaacucaag      240

cacguguguc ccuacgagcu gagaguaaag ggucuucaug aaaaacugac gcaaggggag      300

auuuauaagg uggucuugca cauccucaaa aagaggggaa uaucuuaccu ggacgagucg      360

gaugaaaacu caagcggcau cgacggcgca aguuauaaag aucaggugaa ucugaaccuc      420

aauuuacaga aggagcugac cccugggcag auucaacuua agcgguugaa agaaaacgga      480

cgcaucaagu ccgguguuac auucgagaau ggcuaccagc ugaacguguu uucugucagc      540

gccuaugcga augagcuaaa caaaauucuc acuaagcagu ccgaauacua cucagagauc      600

gaccagauau ggauugaucg auucaccaaa aacggguaua gaaaggaagc uggacuggug      660

uacaggaaac ggccauauua cgacggcccg gguaaugaga agaacaauag uaucuauggg      720

cguugggcca acuuuaagga agauggacau cccaaagaga auauuuucga ccaacuuauc      780

ggccuggaca gcguagauuc uacacagguu cgcgcauccg ccucaaccuu gacugcucag      840

aaguacaacc uccugaauga cuuaaacaau cugacaauca uugaggauga cagaaaauuu      900

accacggaug aaaaggugag cauaaucaac uaucucauga cucaggacau uaccagguuc      960

ggcccuaaag agcuugcaaa guuuuaccaa cuggaagucg aucagauccg gggguggcga     1020

auagacaaua agggaaaccc agagauucac aguuugaaag uguaccgcga uuggaagaaa     1080

aucuucaaua caucuuccau cgaccuaacu agcauucaga ccuggguggu cgaugaacug     1140

gccaacguua ucacacucaa uaccgagauu cccucgacgc uggcuacucu uaagauaaaa     1200

aucccugaca uugaccaaca gauccugaac uuuguggaga acaauuucua ugaauugaag     1260

aagaaaacau cuaagaacuc cuggcauuca uuuagucuca aaacccugaa uaucuuaauu     1320

ccagagcugc uaaacacuag cgaagaacag aauacaauac ucgaaagacu gaaccuuaag     1380

uucgauuuga aaaauaagua cgccgagaag aaccagaucc ccguaaaaga gcugcucgaa     1440

caaauuuaua auccuaccgu gacgaagucc guccaccagu cuaucaaacu guucaacgag     1500

auuaucgaaa aguacgguaa aaauaacaua agcuauguga ccaucgagau gccaagggac     1560

aagaaugauc aggaccagaa agauacaauu aagcggauca acaaacuuaa uucagagcgc     1620

aaaaacgaca gugaaaagua cuuucuggag agauccggcu gggauacuau uagguucgaa     1680

agcgcguuga aaaagccgcg uuuugcagag aaacuccugu ucuauuacga acaaaagggg     1740

aagugcgccu acucuggaca ggacaucuua gcugagucac ugcuaaacga gaguugugaa     1800

guugaucaca uaauuccccu cagcaucucc uuugacgauu cuauucauaa uaaagugcug     1860

gucaccgccu cgagcaacca ggagaagggc cagcggacac cuuauauggc auucaucgac     1920

ggugcuaaac uugggcaauc cugggaugac uuuguggccu ggguaaauuc augcggcuuc     1980

caccgaaaga aaguucagaa cuugcuguac gaagagaaua uauuuaaccc agacgugcag     2040

agaggauucg ucaguaggaa ucucaacgau acucgcuaug cgucucggau cgugcugaau     2100

gccauucaga gcuuuuucua caagcaaaag gacuuaacca aagucaaggu gguuaacggc     2160

uccuuuacac auacccugag gaaaaagugg gggucaagcc uugauaaaaa uagagacacu     2220

uaucaccacc augcagugga ugcuacguug ugugccguaa cacccuucau caacauuguc     2280

aaguacgagu ucuuugagga ggacggaauc aaguauaugg uggaucucac cacuggugaa     2340

aaaguugcuu acucugacua caagaaaaug aaguucuaug auaaacgcgc cuacgugccu     2400

auaugggacg gcuuuauuaa gcagcugacc ccagcaaauc uauaucccaa aaucaaguuc     2460

ucccaccagg ucgauaguaa guggaaccga aaaaucagcg acgccacaau uuacucuacc     2520

cggaagaucg uggagacuaa aauuaauaag aaagggguag cuaaggaaac agugaaggau     2580

auaguuaucg acaaaauuaa ggacaucuau acgccggagg gauggcguaa auuuaaggaa     2640

aaacaagaua agcugcucau gaagaacuuc gacgagaaaa ccuacaacaa gcugauuaaa     2700

aucgcgaagg aguauccuga gaaaaaggaa guccagacuu ccaauggcaa ggugucacuu     2760

guggaaguca gucccuuuaa aauguacugc gaggagcaga acguuccagc aauaagaaag     2820

uacagcaaaa aggguaaugg gccuuauauc aggucguuga aauucuacga uaaggaacug     2880

ggcaagcaua uuaacaucac aaaagacucc uauggaaaug auauugagcg caccauccgg     2940

aacaagaaaa uaauuuuaaa gaaucagaac ucuuggagaa cagacgugua cuauaauagc     3000

gugaacaauu guuacgaacu ccugggcauc aaauauugcc accuuaaguu uuacaggggg     3060

aaauacggaa uccccaaguc aauuuaugau gagcugaaga aagaagacga gauaaguucc     3120

gagucuguau ucaaguuuag cuuguaccga caugaucgcg uccgguucga aaaagacggu     3180

gaguauaucg aagcccucuu uggcuccaag aacgagucaa auaaaaacua ccuggaacua     3240

agcccaauug auaagaauaa guggcugagu aaagagaacg ugaaguucuu cggggagguu     3300

gcugccucug gaagauuuau caaaggccuc aagcccggua uuaaagugau caagcaaucc     3360

acugacuaua uggggcacgu cuacgcaaua caccaggaaa agcugaaauu cgugcuuagc     3420

gacgccuga                                                             3429


<210>  2850
<211>  3435
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2850
augaccagca agcaggagaa acacggcgcc cugacauucg ggcucgacau cggaauugcu       60

uccguggguu gggcaguccu uggcgaaucu agaauaguug aucugggggu gaggugcuuu      120

gacaaggccg agacugcgaa agaaggagag ucauugaacu uaacgcggcg cauggcucga      180

cugcuacgua gaaggcuccg gcgcagagcc uggaggcuga ccaaacuugc acggcuguug      240

aaacgcgaag gccucaucgc cgaugcuaau cuguucaagc auaagauuga gaaaaacaca      300

cccguaccua agaguagcug gcaacugcga guggacgguc ucgauagaca guuaaaugca      360

gaggaauggg ccagggucau cuaccaccug uguaaacauc ggggguuuca cuggauuucc      420

cgugcugagg aaaaggccgc ggagggagac ucuaaaggcg agggcgggaa ggugaagcag      480

ggacuugcag guacuaaaaa guugaugcag gaaaaaucgu aucgcaccgc cgcugagaug      540

guucuggccg aguucccaga ugcacaaaga aacaagcagg gcgacuacuc aaaagcucua      600

agcaggaucc uccuggggga ugagcuuaag gcccuguuug aacaucagcg gaaauuggga      660

aauccggugg cgacagagaa gcucgaggcc gcaauacugg gcaacgguga caagaaaugc      720

ggguuauucu gggcucagaa ucccgcccug gcuggaaaca agcuccuuga aaugcugggc      780

cgauguaccu uugagaaaac uggcggggau gacggaaagg gugaauaucg cgccccuaaa      840

gcaaguuucu ccgccgagag acacgucugg uugacacacc uaaauaaccu gaggaucgug      900

guagauggca ccacgcggcc acucaaugaa gcugagcgca gaguugcgcu gcccaugccu      960

uaccaacagg caggggaccu uucuuauaag cagcugggag gcgccuugau uaaggagggu     1020

uuucuccaga aagacggguu caaguuugcu ggccugccau accccacuaa acaagccagc     1080

gaagagaagg caaaagaucc ugaaaccucc guguuaugca agcugccagc cuggcauaca     1140

cuaaggaaga cucucgagaa acacggacug gaaucagagu ggcagaaacu uaguaccgac     1200

gcuuuggaug gcaagccgca gcugcucgac aacaucgccu ggguccugag cguguauaag     1260

gaugacgaug aggugaaaac agaacuuggg aagcugaauu ugcccaacac cgagaagaug     1320

auugacgcac uccuggauau ccguuucgac aaauuuucua auuuaucccu gaaggcgcua     1380

ucaaaaauag ucccuuucau ggaacaagga cagcgguacg augaggcugu ugccaacauu     1440

ccagaguacg gucaucacag ccagcuccac aaggugggcg caggggaaca uaaauaucug     1500

cccccuuucu acaaggagcg agacaaaacg ggaagaaugg uauuuaacga cgauauggac     1560

aucccaagga aucccguggu ccuucgcgcc uugaaccagg cucggaaggu gguuaaugcc     1620

cugauuaagg aauauggcag uccgcacgag gugcauaucg aaauggcaag agaucucucu     1680

aggccucugg acgagcgccg agauaucgag aaauuacaaa aggaguucgg ugaacggaac     1740

aaaaaggaca aagaacuguu ugcucaggau uucaauauug ucgggacugu gaagggcaag     1800

caguuugaga aauaccagcu uuaucgugaa caacagggaa aguguauaua ccccuccaug     1860

gacgagaaac agggcaucug cuguuauagc uugaagccac ucgaucugca cagaguacua     1920

uacgaggaag gguacgccga gauugaccau gcgcugccuu auucgagguc uuucgaugac     1980

uccaaaaaca aucgcguucu cgugcuggcc aaggaaaacc gggauaaggg aaauagaaca     2040

cccuuugagu accuugguga caaagacaac ucagaaccau ggaggcaguu cgucgcuuuu     2100

guggagggca auaaggcaua ucgauuggcc aaacgcaguc ggcuguuaag aaaggauuuc     2160

ggggacaaag aggcuaagga auuuagggag cguaaccuca augauacccg cuacaucugc     2220

aaguucuuua aaaacuaugu cgaacaauac cugcagcuuc acgaggacag cgaggccaag     2280

cggugugugg uucuguccgg acagaugacu ucuuucuuga gagcaaggug gggccucgug     2340

aaaguacgag gugauagcga ccggcaccau gcgcuggaug ccgcugucgu ggccgcaugc     2400

ucacacggga ugguuaagcg ccuaagugac uauuccagaa ggaaagaacu ggaacaggug     2460

cgggaaggcu ucgucgaugu ggacacagga gagauaguaa aucccgcuau guuucaacag     2520

gugcaggaac auuucccuaa cccauggccg cacuuucgag augagguuaa gcucagacug     2580

agcauugacg aucccacccu uuugcgcucu gagauggaaa aauucggcac guacaccccu     2640

gagucacugg aagccuuaag gccacucuuu gucagucgug caccccaacg gagaaacagc     2700

ggggccgcuc acaaggacac aaucuacgcg cagccuaaga ggcugguggc ccagggaggc     2760

gugacucaga aagucgcacu uuccucucug acccuaaagg accucgauaa acugaucgac     2820

ccacaucgca augagcgauu guaugcugcc auuagagccc ggcuggaaca acacgguggg     2880

aagggagaua aggcuuuccc cgcaucgaac ccguuaagga agccugacag cgagggcaau     2940

ccaacagguc ccaucguucg cggcgugacu auggugauug auaagcucuc cgggauaccu     3000

auccguggag gcauugccaa aaacgacuca augguaagag ucgauguguu uaccaaggcg     3060

aauaaauucc aucugguucc cguguacguc caccauagug ugcuugggcu gccaaaccag     3120

gcuaucguug gaggcaagga ggaaucugag uggacacaga ucgacgauag cuuugacugg     3180

cacuucucca uuuauccuaa ugauuugaua cgggugaccc ucaaacagga agguagguuu     3240

ggguacuaug ccggauguga ccgcucaacu gcagccaucc accugugggc ucaugaccga     3300

aaccaacagg uaggcaagga uggucuaauu agaagcaucg gcgucaagac ggcacugucu     3360

auugagaaau ucaaugugga cgugcuuggg aaccgguacc ccgccccacc ggaaaagagg     3420

cgcgaucucg cguga                                                      3435


<210>  2851
<211>  3435
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2851
augaccagca agcaggaaaa ccugggcgcc cucacauucg ggcuugacau cggaauugcu       60

uccguggguu gggcaguccu gggcgaaaau agaauaaucg auuugggggu uaggugcuuu      120

gacaaagccg agacugcgaa ggaaggagag ucuuuaaacc ugacgcggcg cauggcucga      180

cuacuccgua gaaggcugcg gcgcagagcc uggaggcuua ccaaacuggc acgguugcuc      240

aagcgcgaag gccugauugc cgaugcuaau cuguucaaac acaagaucga gaagaacaca      300

cccgugccua aaucaaguug gcaacuccga guagacgguu uagauagaca gcugaaugca      360

gaggaauggg ccagggugau uuaccaucuu uguaagcacc ggggguuuca uuggaucagc      420

cgugcugagg aaaaagccgc ggagggagac uccaagggcg agggcgggaa agucaagcag      480

ggauuggcag guacuaagaa acugaugcag gaaaagucuu aucgcaccgc cgcugagaug      540

gugcuagccg aguucccaga ugcacaaaga aacaaacagg gcgacuacuc gaaggcucuc      600

ucaaggauac ugcuugggga ugaacugaaa gccuuguuug aacaccagcg gaagcucgga      660

aauccgguug cgacagagaa acuggaggcc gcaaucuuag gcaacgguga caagaagugc      720

gggcuguucu gggcucagaa ucccgcccuc gcuggaaaca aacuucugga aauguugggc      780

cgauguaccu uugagaagac uggcggggau gacggaaaag gugaauaucg cgccccuaag      840

gccagcuuca gugcagagag acaugugugg cuaacacacc ugaauaaccu caggauuguc      900

guggauggca ccacgcggcc acugaaugaa gcugagcgca gaguagcgcu ucccaugccu      960

uaccaacagg caggggaccu guccuauaaa caguugggag gcgcccucau caaggagggu     1020

uuucugcaga aggacggguu caaauuugcu ggcuuaccau accccacuaa gcaagccucu     1080

gaagagaaag caaaggaucc ugaaaccagc guucugugca aacuaccagc cuggcacaca     1140

cucaggaaga cucuggagaa gcauggacuu gaauccgagu ggcagaaauu gucaaccgac     1200

gcucuggaug gcaagccgca gcuccuggac aacauugccu gggugcuuag ugucuauaaa     1260

gaugacgaug aggugaagac agaacugggg aaauugaauc uccccaacac cgagaagaug     1320

aucgacgcac uguuagauau acguuucgac aaguuuagca accugucucu aaaagcgcuc     1380

uccaagauug ugccuuucau ggaacaagga cagcgguacg augaggcugu cgccaauauc     1440

ccagaguacg gucaccauuc acagcugcac aaaguuggcg caggggaaca caaguaucuu     1500

cccccuuucu acaaagagcg agacaagacg ggaagaaugg uguuuaacga cgauauggac     1560

auuccaagga aucccguagu guugcgcgcc cugaaccagg cucggaaagu cgugaaugcc     1620

cucaucaagg aauauggcag cccgcaugag guucacaucg aaauggcaag agaucugagu     1680

aggccuuuag acgagcgccg agauauugag aagcugcaaa aagaguucgg ugaacggaac     1740

aagaaagaca aggaacuuuu ugcucaggau uucaauauag uggggacugu caaaggcaag     1800

caguuugaga aguaccaguu guaucgugaa caacagggaa aauguaucua ccccucuaug     1860

gacgagaagc agggcauuug cuguuauucc cucaaaccac uggaucuaca uagagugcug     1920

uacgaggaag gguacgccga gaucgaccac gcgcucccuu auagcagguc guucgaugac     1980

ucuaagaaca aucgcguacu gguucuugcc aaagaaaacc gggauaaggg aaauagaaca     2040

cccuuugagu acuuggguga caaggacaac uccgaaccau ggaggcaguu cguggcuuuu     2100

gucgagggca auaaagcaua ucgacuggcc aagcgcucac gguuacucag aaaagauuuc     2160

ggggacaagg aggcuaaaga auuuagggag cguaaccuga augauacccg cuacauaugc     2220

aaguucuuua agaacuaugu ggaacaauac cuucagcugc augaggacag ugaggccaaa     2280

cggugugucg uguugagcgg acagaugacu uccuuccuca gagcaaggug gggccugguu     2340

aaggugcgag gugauucuga ccggcaccac gcgcuagaug ccgcuguagu cgccgcaugc     2400

agccauggga uggugaaacg ccugucagac uauaguagaa ggaaggaacu cgagcagguu     2460

cgggaaggcu ucguggaugu cgacacagga gagauuguga accccgcuau guuucaacag     2520

guacaggaac acuucccuaa uccauggccg cauuuucgag augaggugaa acugagacuu     2580

uccaucgacg aucccaccuu gcugcgcagc gagauggaaa aguucggcac guacaccccu     2640

gagucuuuag aagcccucag gccacuguuu guuucacgug caccccaacg gagaaacagu     2700

ggggccgcuc acaaagacac aaucuacgcg cagccuaaga ggcuugucgc ccagggaggc     2760

gugacucaga agguggcacu gagcucccua acccucaaag accuggauaa guugauugac     2820

ccacaccgca augagcgacu guaugcugcc aucagagccc gguuagaaca acaugguggg     2880

aaaggagaua aggcuuuccc cgcaucuaac ccgcucagga aaccugacuc ggagggcaau     2940

ccaacagguc ccauuguccg cggcguuacu auggugauag auaagcugag cgggaucccu     3000

auucguggag gcaucgccaa gaacgacucc auggugagag uagaugucuu uaccaaagcg     3060

aauaaguucc accuugugcc cguuuacgug caucacucag uccugggguu gccaaaccag     3120

gcuaucgugg gaggcaaaga ggaaagugag uggacacaga uugacgauuc uuuugacugg     3180

cauuucagca uauauccuaa ugaucucauc cggguuaccc ugaagcagga agguagguuu     3240

ggguacuaug ccggauguga ccgcuccacu gcagccauuc accuaugggc ucacgaccga     3300

aaccaacagg ugggcaaaga uggucugauc agaucaauug gcguaaagac ggcacuuagc     3360

aucgagaagu ucaaugucga cgugcucggg aaccgguacc ccgccccacc ggagaaaagg     3420

cgcgaucugg cguga                                                      3435


<210>  2852
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2852
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc ccuggugaaa       60

gagacugaaa auuccaacga gaagucugaa auaaucaaau ugggugucag aguuaauccc      120

uuaacggugg augagaagac caacuucgaa gcuggcaggc cucugucaac aaaugcagac      180

cggacugcca aacgcagugc gcgacguaac cuacagagau acaagcucag gcggaagaau      240

cugauugauc uucugaucaa acaccgcuug auugacaagg auaccccacu cacagagauc      300

gggaaaaaca ccacucauca aacacuggag cugagagcua aggccgcaag ggaacggaua      360

gagcucgaag acuuagcccg cguauuucug gcuaucaaua aaaagcgagg auauagaagc      420

uccaggaagg ugaacaauga ggaggaaggc caggucgugg augguauggc aguugccaaa      480

aaguuguacg acgagaaccu uaccccgggg caguaugcuu acgaacugcu aucuaaagga      540

aagaaauaug ugcccgauuu cuaccggucg gaccuccagg ccgaguuuga uucaauuugg      600

gaauaucaaa agcaguucua cgcggacauc cuggaugacg agcuuuacaa ugcacugaaa      660

ggccagggcc agcaaaacag ccguaagcgc uuuuuggcca uuaagggggu cuauacggcu      720

gagaauaaag gaaagagaga cgaagugaaa cuccagcacu acaaguggag gagugaggcc      780

aucacucaga aacuguccau agaagaggua gcauauguuu uaguggaaau uaacaacgau      840

cugaauaagu cuagcgguua ccucggcgcu aucuccgacc ggucaaagga gcuuuauuuc      900

aacaaagaga ccgucgggga aaaucugugg aagcagauuc aaaagaaccc ucauacaagu      960

uugaagaauc agguguuuua ccgacaggau uaucuagacg aguucgaaca aaucugggag     1020

acucaggccc aguuucaccc acagcugacc cucgcgcuga aagaacaaau ccgcgaugug     1080

gucauuuucu accagagaaa acuuaagagc cagaaaggac uguugucuuu uugccaguuc     1140

gaguccuggg agauagaaag gaaggacgag aacggcaaug uuauccucaa caaaacaacc     1200

caacugccca agcggcagac ggugggucgc agaguagccc cuaaaucaag cccauuauuu     1260

caggaguuca agauuuggca gaauaucaac aaucuggaga uagcaaagau uagugauggg     1320

aacucuaaaa auaagaaaga gacugaagcu cuauccgacg augagaggaa gcuccuguuc     1380

gaagaacuua acuugcgugg aaaucugagc caaaaagaag ugcuccagau ccugggccuu     1440

aaggacaaag aguacaagac aaacuuuccc gagggccugg aagggaaccg gaccaaugcc     1500

gcuuuguuca acaucuauca gcagauugcc gagaaugaag gauacgguga uuggacuaag     1560

aaaucggcac aagagaucaa ggaggaacuc aaagccgucu uuccucagau uggcauacag     1620

gcugacaucc uggacuucaa cgcggaguua gaugggaagg aauuugagaa ucaagcaucu     1680

uaucagcugu ggcaucuacu cuacuccgcc gaagaggacg auaaaauuaa cgaagaggac     1740

cagaucaucu auggaaauuc agcugugagu cugaagaaga aacuuuguga gaaguucggc     1800

uuuacaccag aauacgccaa auggauugca aacguuagcu ugcaggauga cuaugguaau     1860

cuguccacca aggccaugcg aaaaauaauc ccguaccuca uugaugggaa cgacuacucu     1920

gaggcuugcg cccuggcagg cuauaaucac agcaacucau uaacgaagga agagaaugau     1980

aacagagaga ggcugaauaa acuugaauug cuccccaaaa acagucugcg caacccugug     2040

gucgagaaga uccuaaauca aauggugaac guaguuaauc aggugauuga aaccuacgga     2100

aaaccagacg agguccggau cgaacuggcg agagagcuca agaaauccgc ugaggaaagg     2160

gccgagauga caaagggcau aaacgaagca acucgccgaa augaggauau caagaaacug     2220

auuaccaaga acuucgggau ccccaauccu acaaagaacg acgugguccg guaucgucuu     2280

ugggaagagu uggccccacu ggcuuacaag gauguguuua cuggaagaca gauuaaaaag     2340

gaggacuuau ucagcucuaa aaucgacaua gaacacauua uccccaaggc ccuccuguuu     2400

gaugacucau ucaguaauaa gacccuugca uuuagggaga caaaccugaa aaaggcugau     2460

cgcaccgccu augguuucau ugaaagcgac uacaaugcga cuuuggauga cuauauccag     2520

cggguugaga cgcucuacaa caaugccaaa ggcacaauau ccaaggggaa aagaaacaag     2580

cugcuaaugg cucaaaagaa ucugccggau ggauucaucg aaagggaccu ccgaaacucu     2640

caguacauug ccaaaaaggc aaaaucgaug aucgagaagg uguuuaacga uguaaauguc     2700

accagcggcu ccauuacuga ccgccugcgg gaggauuggg accuuaucaa cgugaugaaa     2760

gaauugaauu ucccuaagua uaaggcucug gguuuaaccg agauagaaga gagauacgau     2820

auugggcagg agaaaacaaa gaaaguugaa gugaucaccg acuggacuaa gaggaacgac     2880

caucgucacc augccaugga ugcacucaca gucgcguuua cgaccaaauc acacgugcag     2940

uaucugaaua accuuaauac uauccacagu cugaagggcg acgagguaga ucuagaacuc     3000

ucuaagcugu acggauugaa aaacacaauu accgaggugg uuaauaagaa acgcaaguuc     3060

auucccccaa ugccuaacuu ucgggaagag gccaaaaagc auaucgagac aauacugguc     3120

agcaucaaga auaaaaacaa gguguauacu aaaaauauua acaagaccaa aaaggaaucc     3180

ggcuaccaca cgaaagagca auuaaccccc agagggcagc uccauaagga aacaguguau     3240

ggaaagucaa aaaggccaau gaauaagccc acuaaaauca acaagaauuu cagccuggag     3300

caggcucaac uuauuaucaa cguccaggaa aaagagcugg uuuugcgaca ccucgcccag     3360

uuugacaaca auccugcagu ggcuuucgau accaagacac ugaagaaaug ucauuaccua     3420

aagaugguga accuguga                                                   3438


<210>  2853
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2853
augagcggca acacccacgg gccccugaca uucggauuug acaucgguau ugccuccgug       60

ggcuggugcg uccucgggga gaauagaaua guuaaccuug gagugaggug uuucgauaag      120

gcugaaacug caaaagaggg cgaaucucug aauuuggccc ggcgcacggc gcgauuaaug      180

cguagaaggc ugcggcgcag agcuuggagg cuaaccaagc ucgcccggcu gcuuaaacgc      240

gagggucuga ucgcagaagc cucacgaauc cauauuccua guccaguaag cccguggaga      300

uugagggugg aggggauuga ccggcgucuc acagaugacg agugggcucg cgucaucuac      360

caccugugca agcauagagg auuucacugg auauccaggg cagaagaacg gaaagccgaa      420

ucugaugcua aggagggcgg ccgagugaag aaagggcugu cggacacuca gcgcagaaug      480

accgagaagg gauauagguc agccgcggaa augguucucg cagaguuccc cgaugcccaa      540

cggaacaaac agggugacua cggcaaggcu uuaagccgca uccugcuuag ugaugaauug      600

gcccugcuau uuagaaggca gcgugagcuc gggaauccua acgcaggaaa ugaacuggag      660

gcuacaauuc uuggcaacgg uaaucggaaa uccgggcugu ucugggagca gaagccaccc      720

uuggccggag cggaccuccu gaagauguua ggccgaugua ccuuugagaa aaacgaguau      780

agagccccua aggcaucuuu cacugcugaa aggcaugugu ggcugacacg ccucaauaac      840

cuucgggucg ugguagaugg caccacgaga agccuggacg augccgagca cagguugguu      900

cuaccacugc ccuacaaaca agcuggggac uuuacuuaua agcagcuccg caccgcccug      960

acaaaagcag gacuucugcc ugaagguuuc aaguuugccg gcuuguccua cccaacuggg     1020

cgacagcucg acgauccgaa agcuaaggac cccgagucag agaagcuggu gcgguuaccu     1080

gcguggcagg aacugcguaa aacccuaaag gauaaaaauc ucgagacaga augggaggga     1140

auggcaggcg ccgcuaccgg ugggaaccca gaacugcuug acgagaucgc cagagucuug     1200

aguguguuca aggauggcga cgaggugacg gcagaacugu ggaaacuccc ccugccugga     1260

ggcaaggcca ugauugaugc ucuuagcgag aucucuuuug acaaguucca cgcccugucc     1320

uugaaagcac ucaggaagau agucccacau auggaaucag ggcugcgcua ugaugaggcg     1380

ugcgcuguug ccggauacca ccauagccaa uuacacgcag ccggugcugg cgagcacaaa     1440

uuccugcccc cguuuuacag ucggagagac aaggauggga ggaugguguu caaugaggaa     1500

cuagacguac cucgaaaccc cgugguccuc cgcgcccuga accaggcacg gaaagugcuu     1560

aaugcuuugg uuagaacuua uggaucucca acagccgugc auauugaaau ggcgagggac     1620

cugucccguc cucucgauga gcgcaacaag gucaagaaag cccaggacga auaccgggag     1680

agaaaugaga aggauaaagc ugcauuugcc gcugaauaug gcaucgcccc caagagcagg     1740

gaguucgaaa aauuucagcu guaccgagag caacagggua agugugcaua uucguuaaag     1800

ucucuggacc uucaccgggu guugcaugau auuggguacg cggaaguaga ccacgcccuc     1860

ccauauuccc gcucauacga ugacaguaaa aacaauaagg uucuggcucu aaccgaggag     1920

aacagaaaua aaggcaacag gacugccuac gaauaucuga ccagcuuccc cggaggcgag     1980

aauggggaac gguggcgaaa cuuuuccgug gcagucgaga caaauaaggc uuacagacuc     2040

gccaaacgca cgaggcugcu ucguaaggau uucucuggac gggcagccga agaguuuaga     2100

gagaggaacu ugaaugacac ccgcuauauc ugccgauucu uuaaaaacac aguggaaaga     2160

uuccugaagu uagaugcuaa ggucggugcg ggcgacacug ccgagaucag cgcaaaacgg     2220

ugugugguuc ucucagggca gaccacagcu uuucugaggg cccgcugggg acuucugaag     2280

gugcguggca auagugauag acaucacgcc uuggacgcug caguagucgc cgcgugcucc     2340

cacgguaugg ugaaacggcu cggggacuac agcaggacua aggaacuggc uaaaguucau     2400

gauggcuucc cugacccaga gaccggagag auuauagauc cggugauguu ccaacagcua     2460

cacgcccauu uucccgaccc uuggccacac uuccgcgcag aacuggaggc ccgacucaac     2520

gucgaugaca cagcugaucu gagagcacgg auggaacuuu uuggcaccua ugcccccgag     2580

gcguuggaaa cucugaggcc uuuauucgug ucucgcgcuc cacagagaag gaacucaggg     2640

gccgcacaca aggacacgau cuacgcccag cccgagcggc ucaaggagca aggaggcgua     2700

acacagaaag ugccgcugac cagucuuacu cugaaggauc uagacaaacu cauugauccu     2760

caucguaaug aaaagcugua ugcugccauc cgcgcacgau uggaggcuca cggugggaaa     2820

gccgacaagg cguuuccagc cggaaacccc cugagaaagc cugauaggga aggcaauccc     2880

accgguccag uuguccggac agugaccaug gugauagaca aauuaagcgg cauuccuguc     2940

cgcgggggaa ucgcaaagaa cgacacuaug cucagaguug auaucuucag gcauaaaaag     3000

gacggcaaau uucaccuggu gcccguguac guauaccaug cugucgccaa ggaguuccca     3060

aaucgggcua uugugcaggc aaaggaugaa gacgagugga cacuuaucga ugacaaauuu     3120

gauuucuguu uuuccgccca cccgaacgac cuguugaaga uuacgcagaa aucuggggag     3180

accauauucg gauauuaccg aggcugccac ucggguacug ccgcuaucaa uauugcgcuc     3240

caugauagau augcauacgc cgaaaagaca accagccugg cuuuuuccaa aaagaacccu     3300

gggaaaccca ucccaaauga caagcuagga cugaucgagg gcauuggugu uaagauggcc     3360

gcaaacaugg aaaaauucca cguggauaca cuuggcaaua uauaucccgc cccuccagag     3420

caacgcaggg ggcucgcuug a                                               3441


<210>  2854
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2854
auggccuaca gacugggccu cgacaucggg auuaccagcg ugggaugggc ugucguugca       60

cuugagaagg augaauccgg ucugaaaccc gugaggauac aggacuuggg cguacggauc      120

uucgauaagg ccgaggacuc uaaaacaggg gcgucauuag cucugccucg ccgagaagcc      180

cguagugcaa gaaggcggac ucgcagaagg cggcaccgcc uauggcgagu gaagagacuc      240

cuggagcaac augggauucu uagcauggaa cagaucgagg cccuguaugc ucagaggacg      300

uccucuccag augucuacgc auugcgggug gccggccucg accguugccu gauugcugag      360

gaaaucgccc gcguucugau acacaucgcg cauagaaggg guuuucaguc gaaccggaaa      420

ucagagauua aggauagcga cgcagggaag cucuuaaaag ccgugcaaga aaaugagaac      480

cugaugcaga guaagggaua ucgaaccguc gcugagaugc uuguguccga agccacaaaa      540

acugaugcag agggcaaguu gguacacggc aaaaagcaug gguacguuuc uaaugugcgc      600

aacaaggcug gagaauauag acacaccguc agcaggcagg ccaucgugga cgaggugcgg      660

aaaauuuucg cggcccagcg cgcacugggu aaugauguca uguccgagga acuagaggac      720

ucauaccuca agauccugug uagucaaaga aacuuugaug acggcccggg aggagacagc      780

cccuauggcc acgguucugu uuccccugau ggggugaggc agucaauaua cgaacguaug      840

guaggaagcu gcacauucga gaccggcgaa aaacgggcuc cacgaaguuc uuacuccuuu      900

gagagauucc agcuucugac uaaggugguc aauuugagga uuuaucgcca gcaagaagac      960

ggcgggcggu accccuguga gcucacacag accgagagag ccagggugau cgauugcgcu     1020

uaugaacaga cgaaaauuac uuacggaaag cugcgcaaau uacuggacau gaaggauacc     1080

gagagcuuug ccggucucac auauggccuu aaccgaucgc ggaauaagac ugaagacacc     1140

guuuucgugg agaugaaauu uuaccaugaa guccguaagg cacugcaaag agccggggug     1200

uucauccagg auuugucuau cgagacacua gaccagauug gauggauacu guccguaugg     1260

aaaucagaug acaacaggcg caagaaacuc aguacccugg gccuuagcga uaauguuauc     1320

gaggaacugu ugccucucaa cgguuccaag uuugggcacc ugucuuuaaa ggcuauucgg     1380

aaaauccugc cauuccuaga ggacggcuau agcuacgacg uggcguguga acucgcagga     1440

uaccaguuuc aaggcaagac ggaguauguc aaacagagac ugcuuccccc uuugggggag     1500

ggagaaguga cuaauccagu cgugaggcga gcccugucac aggcuauaaa gguugugaac     1560

gccguaauuc gcaaacaugg uaguccggag uccauccaca ucgaacucgc acgggagcug     1620

agcaagaauc uugaugaaag aaggaagauu gagaaagccc agaaggagaa ccagaaaaac     1680

aaugaacaaa ucaaggacga gauucgugaa auacugggcu cugcucacgu cacagggcgc     1740

gauaucguga aauacaaguu guucaagcag cagcaagagu ucugcaugua uucaggagaa     1800

aaacucgacg uuacccggcu guuugagccc ggcuacgccg agguggauca uauuaucccu     1860

uaugguauca guuucgacga uagcuacgac aacaaggucu uagugaaaac ugaacagaau     1920

agacagaagg ggaacaggac accacuggag uaucuacgag auaaacccga acagaaggca     1980

aaauuuauug cgcucguaga guccauaccu cugucucaaa agaagaaaaa ucaccuuuug     2040

auggacaagc gggcuaucga ucuggaacag gagggcuucc gcgagagaaa ccucucggac     2100

accagguaca uuacgcgggc ccugaugaau cauauccagg caugguuacu guuugaugaa     2160

accgccagca cacgauccaa aagagugguu ugugucaacg gagcugugac ugccuacaug     2220

cgcgcaaggu ggggccuuac caaggaccgu gacgcugggg auaaacacca ugccgcggac     2280

gccguggucg uugcuugcau uggagauuca uugauccaac gggugacaaa guaugacaag     2340

uucaaaagaa augcacucgc cgauaggaac cgcuacgugc agcagguaag uaagucugag     2400

gguauaacuc aguaugucga caaagaaacc ggcgaggugu uuacauggga gagcuucgau     2460

gaacgaaagu uucugccaaa ugagccccua gaaccguggc cuuucuuuag agacgagcug     2520

cucgcucggc uguccgauga ccccuccaaa aacaucaggg ccauugggcu uuugaccuac     2580

agcgaaacug agcaaaucga uccaauuuuc guuucucgca ugccuacgcg uaaggugaca     2640

ggagcagcgc acaaggagac caucagauca ccccggauag ucaaagugga cgauaauaag     2700

ggcacugaaa uucagguugu gguaaguaaa gucgcccuga ccgaguuaaa gcucacaaaa     2760

gacggugaaa ucaaggacua uuuuaggcca gaggaugacc cccgccugua caacacccuu     2820

cgagaaagac uggugcaguu cgggggcgau gcuaaggccg cauucaaaga gccuguguau     2880

aagauuagca aagacggauc uguccggacu ccaguuagga aggugaaaau ccaggagaag     2940

uugacacucg gcguaccggu gcacggggga cgcggcauag cugaaaaugg ugggaugguc     3000

agaaucgaug uguuugccaa aggaggcaag uacuacuucg uucccauuua uguggcagac     3060

guccugaaga gggagcuacc uaaccggcug gccacggcuc auaaaccaua cuccgaaugg     3120

cgugugguug augacucaua ucaauuuaag uucagucucu accccaacga ugcggugaug     3180

aucaaaccua gccgcgaggu cgacauuacc uauaaggauc gaaaagaacc aguagguugu     3240

agaaucaugu acuuuguguc ggccaauaua gcauccgcuu cuauuagccu gaggacucac     3300

gacaacucag gcgagcuuga gggguuggga auccagggcc uggaaguuuu cgagaaguau     3360

guggucgggc ccuuaggaga cacacauccg guguacaagg aacggcgcau gccuuuuaga     3420

guagagagga aaaugaauug a                                               3441


<210>  2855
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2855
auggccuaca gacugggccu cgacaucggg auuaccagcg ugggaugggc ugucguugca       60

cuugagaagg augaauccgg ucugaaaccc gugaggauac aggacuuggg cguacggauc      120

uucgauaagg ccgaggacuc uaaaacaggg gcgucauuag cucugccucg ccgagaagcc      180

cguagugcaa gaaggcggac ucgcagaagg cggcaccgcc uauggcgagu gaagagacuc      240

cuggagcaac augggauucu uagcauggaa cagaucgagg cccuguaugc ucagaggacg      300

uccucuccag augucuacgc auugcgggug gccggccucg accguugccu gauugcugag      360

gaaaucgccc gcguucugau acacaucgcg cauagaaggg guuuucaguc gaaccggaaa      420

ucagagauua aggauagcga cgcagggaag cucuuaaaag ccgugcaaga aaaugagaac      480

cugaugcaga guaagggaua ucgaaccguc gcugagaugc uuguguccga agccacaaaa      540

acugaugcag agggcaaguu gguacacggc aaaaagcaug gguacguuuc uaaugugcgc      600

aacaaggcug gagaauauag acacaccguc agcaggcagg ccaucgugga cgaggugcgg      660

aaaauuuucg cggcccagcg cgcacugggu aaugauguca uguccgagga acuagaggac      720

ucauaccuca agauccugug uagucaaaga aacuuugaug acggcccggg aggagacagc      780

cccuauggcc acgguucugu uuccccugau ggggugaggc agucaauaua cgaacguaug      840

guaggaagcu gcacauucga gaccggcgaa aaacgggcuc cacgaaguuc uuacuccuuu      900

gagagauucc agcuucugac uaaggugguc aauuugagga uuuaucgcca gcaagaagac      960

ggcgggcggu accccuguga gcucacacag accgagagag ccagggugau cgauugcgcu     1020

uaugaacaga cgaaaauuac uuacggaaag cugcgcaaau uacuggacau gaaggauacc     1080

gagagcuuug ccggucucac auauggccuu aaccgaucgc ggaauaagac ugaagacacc     1140

guuuucgugg agaugaaauu uuaccaugaa guccguaagg cacugcaaag agccggggug     1200

uucauccagg auuugucuau cgagacacua gaccagauug gauggauacu guccguaugg     1260

aaaucagaug acaacaggcg caagaaacuc aguacccugg gccuuagcga uaauguuauc     1320

gaggaacugu ugccucucaa cgguuccaag uuugggcacc ugucuuuaaa ggcuauucgg     1380

aaaauccugc cauuccuaga ggacggcuau agcuacgacg uggcguguga acucgcagga     1440

uaccaguuuc aaggcaagac ggaguauguc aaacagagac ugcuuccccc uuugggggag     1500

ggagaaguga cuaauccagu cgugaggcga gcccugucac aggcuauaaa gguugugaac     1560

gccguaauuc gcaaacaugg uaguccggag uccauccaca ucgaacucgc acgggagcug     1620

agcaagaauc uugaugaaag aaggaagauu gagaaagccc agaaggagaa ccagaaaaac     1680

aaugaacaaa ucaaggacga gauucgugaa auacugggcu cugcucacgu cacagggcgc     1740

gauaucguga aauacaaguu guucaagcag cagcaagagu ucugcaugua uucaggagaa     1800

aaacucgacg uuacccggcu guuugagccc ggcuacgccg agguggauca uauuaucccu     1860

uaugguauca guuucgacga uagcuacgac aacaaggucu uagugaaaac ugaacagaau     1920

agacagaagg ggaacaggac accacuggag uaucuacgag auaaacccga acagaaggca     1980

aaauuuauug cgcucguaga guccauaccu cugucucaaa agaagaaaaa ucaccuuuug     2040

auggacaagc gggcuaucga ucuggaacag gagggcuucc gcgagagaaa ccucucggac     2100

accagguaca uuacgcgggc ccugaugaau cauauccagg caugguuacu guuugaugaa     2160

accgccagca cacgauccaa aagagugguu ugugucaacg gagcugugac ugccuacaug     2220

cgcgcaaggu ggggccuuac caaggaccgu aaugcugggg acaaacacca ugccgcggau     2280

gccguggucg uugcuugcau uggagacuca uugauccaac gggugacaaa guaugauaag     2340

uucaaaagaa acgcacucgc cgacaggaau cgcuacgugc agcagguaag uaagucugag     2400

gguauaacuc aguaugucga uaaagaaacc ggcgaggugu uuacauggga gagcuucgac     2460

gaacgaaagu uucugccaaa cgagccccua gaaccguggc cuuucuuuag agaugagcug     2520

cucgcucggc uguccgacga ucccuccaaa aauaucaggg ccauugggcu uuugaccuac     2580

agcgaaacug agcaaaucga cccaauuuuc guuucucgca ugccuacgcg uaaggugaca     2640

ggagcagcgc acaaggagac caucagauca ccccggauag ucaaagugga ugacaacaag     2700

ggcacugaaa uucagguugu gguaaguaaa gucgcccuga ccgaguuaaa gcucacaaaa     2760

gauggugaaa ucaaggacua uuuuaggcca gaggacgauc cccgccugua caauacccuu     2820

cgagaaagac uggugcaguu cgggggcgac gcuaaggccg cauucaaaga gccuguguau     2880

aagauuagca aagauggauc uguccggacu ccaguuagga aggugaaaau ccaggagaag     2940

uugacacucg gcguaccggu gcacggggga cgcggcauag cugaaaacgg ugggaugguc     3000

agaaucgacg uguuugccaa agauggaaag uacuacuucg uucccauuua uguggcagac     3060

guccugaaga gggagcuacc uaaccggcug gccacggcuc auaaaccaua cuccgaaugg     3120

cgugugguug augacucaua ucaauuuaag uucagucucu accccaauga ugcggugaug     3180

aucaaaccua gccgcgaggu cgacauuacc uauaaggauc gaaaagaacc aguaggccug     3240

agacuuaugu acuuugccgg ugcagacauc ucgggcgcua cuuugacacu gaggacccac     3300

gacaacuccg gggaguuaaa gggacucggc auacaggggc uggagguguu cgaaaaguau     3360

guugugggac cccuuggcga uacacauccg gucuacaaag agcggcgcau gccuuuuaga     3420

guggaaagga agaugaauug a                                               3441


<210>  2856
<211>  3447
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2856
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccauaggu       60

uucgccgugg ucgaugcuaa cucucaccuu cugagauuga aaggcgagac agcaaucggg      120

gccagguuau uucgggaagg acaaucagcg gcugaccgcc gaggcagucg uacuacgaga      180

aggcggcuga gccgcaccag auggaggcua uccuuccucc gggauuucuu cgccccucau      240

auuacaaaga ucgacccaga cuuuuuccug cgccagaaau auucugagau uucgccgaag      300

gauaaagauc gauuuaagua cgaaaagaga cuuuucaaug acaggacuga ugcagaguuu      360

uaugaagccu accccucaau guaucaucug cgguugcguc ucaugaccga cgcuagccag      420

gcagauccuc gcgagaucuu ccuggccaua caccacaucc ugaaaaguag aggucauuuu      480

cucacaccag gggcugccaa ggacuucaac accgauaaag uugacuuaga ggacauuuuu      540

cccgcgcuga cugaagcaua cgcccaggug uauccugauc uugaguugac auucgaccug      600

gcuaaggccg augacuucaa agcaaagcua cucgaugaac aagcuacccc auccgacacg      660

cagaaggccc ugguaaaucu ucuguugucu agcgauggag agaaagagau cgugaagaaa      720

aggaagcagg uccucacuga auuugcgaaa gccauuaccg gccugaagac aaaauucaac      780

uuagcacugg gcacugaggu ggacgaagcu gaugccucca auuggcaguu uucaaugggg      840

caacucgacg auaaguggag uaacaucgag accagcauga cagaccaggg aaccgaaaua      900

uucgagcaga uucaggagcu uuaccgggcu cgacuguuga augguaucgu ucccgccggc      960

augucucuau cccaagcaaa gguggccgac uacgggcagc acaacgaaga ucuggagcuc     1020

uuuaaaacgu aucugaagaa acuuaaugac cacgaacugg cuaagacuau ucgcgucauc     1080

uacgauagau auaucaacgg agacgaugcg aaaccuuucu ugagggagga cuuugugaag     1140

gcacucacaa aggaaguaac cgcccaucca aaugagguuu cagagcagcu guuaaaccgg     1200

augggccaag cuaauuucau gcugaaacag cgcacuaagg ccaacggugc aauuccggug     1260

cagcuacagc aaagagaacu cgaucagaua aucgccaacc agagcaaaua cuaugacugg     1320

cuggcuaagc ccaauccugu cgagaaacac agggccagua agccauacca gcuugaugaa     1380

uugcugacau uucguauucc cuauuacgug gggccucuca ucaccccaaa ggagcaagca     1440

aaaucuggag aaaacguguu cgcguggaug guccggaagg accccuccgg caauauaacg     1500

ccguacaacu uugaugagaa aguugaccga gaggcuagcg ccaauguguu cauucagaga     1560

augaagacca cagauacuua ucugaucggc gaagacguac uuccuaaaca gucgcuguug     1620

uaccagaagu augaggugcu caacgaacug aauaacguca gggugaauaa cgaggcauua     1680

gggaccgacc aaaagcagcg ccugauccgg gagguuuuug aaagacauuc uuccgugaca     1740

auuaaacagg ucgccgauaa ucuaguggcu cacggagacu ucgcccagag gcccgagauc     1800

cgcggucucg cagaugaaaa gcgauuccug ucaagucuua gcacuuacca ccaauugaaa     1860

gagauucugc auggcgcuau agacgauucc accaagcucc acgacaucga aaacauuauc     1920

acauggucua ccguauuuga ggaugccgcg aucuucaaaa cuaagcuggc cgagauugac     1980

ugguuagaug cugcaacgau aaagaagcug agcaauaucc gguaucgugg guggggacag     2040

uuuucaagaa aacuuuugga cggccucaaa cuggguaacg ggcagacagu uauucaagaa     2100

cuaaugcuga guaaucauaa ccucaugcag auccugaccg augagacucu uaagaccaca     2160

auggccgaau ugaaccagga caaacugaag gcugaugaca uugaggacgu gaucaaugau     2220

gccuacaccu ccccaagcaa caaaaaggca uuaaggcagg uccuccgcgu ggucgaagac     2280

auaaagcacg cggccaaugg ccaagauccu ucuuggcugu auaucgagac ugcugacgga     2340

cccggcacag ccgggaaacg gacgcaguca agacagaagc agauucaaac cguguacgca     2400

aacgcugccc aggagcuuau cgauagugca guuaggggag aacuggagga caaaauugcc     2460

gauaaggcua gcuucacuga ccgauuggug cucuacuuua ugcagggugg ccgcgauauc     2520

uauacagggg cgccacugaa uauagaccag cuaucccauu acgauauuga ccacauccug     2580

ccccaaucuc ucauuaaaga cgauucgcug gacaaccggg uacuugucaa ugccaccauc     2640

aacagagaaa agaauaacgu guucacaagc acuuuguuug cauccaagau ggcugccacc     2700

uggaggaaau ggcacgaggc cggacugaua ucaggccgua aguuacgcaa ucucaugcug     2760

cggccugaug aaaucgacaa auucgcucau gguuuuguug caagacagcu uguggagacg     2820

aggcagauua ucaagcugac cgagcaaauu gccgcggcuc aguauccaaa cacaaaaauc     2880

auagccguca aggcagggcu aagucaccag cuccgagagg aacuggauuu cccgaagaau     2940

cgggacguga accauuacca ccacgccuuu gaugcuuucu uggcagcccg cauuggcacu     3000

uaucuguuaa aaagauacgc gaagcucgcu cccuuuuuca ccuauggaga auuugccaaa     3060

guagacguga agaaauuuag ggaguucaau uuuaucggcg cacugaagca ugccaaaaag     3120

aacaucguug cuaaggauac aggggagauu gucugggaca aagaacggga uauccaggag     3180

cuugaccgaa uuuacaacuu caagagaaug cugauaacuc acgaagugua cuuugagacc     3240

gccgauuugu ucaaacaaac gaucuaugca gcuaaggacu cuaaagaacg cggagguagc     3300

aagcagcuca uuccuaagaa aaggggcuac ccaacacagg uguauggggg aauccauggc     3360

aagauucguc ugaccuggcg guggcuaaaa cugcuuagaa ggaagaaaau guccaucgag     3420

cucucagagc ugcaguugcu gaaguga                                         3447


<210>  2857
<211>  3450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2857
auggagagau accacaucgg ccuggacauu gggaccagcu ccauaggaug ggccgugauc       60

ggugaugacu ucaagauuaa aaggaagaaa ggcaagaacc ucaucggggu ccggcuuuuu      120

aaagaaggag auacagcugc agagcgccga ggcuuccgua cucaaagaag gcggcugaau      180

cgcagaaagu ggagguugaa guuacuggaa gagauuuuug accccuauau ggccgaaguu      240

gauaaauacu ucuuugcgcg gcuaaaggag ucuaaccucu caccuaaaga caguaauaag      300

aaauaucugg guagccuucu guucccagau aucuccgacu cuaacuuuua cgauaaguau      360

ccgacgauau accauuugcg ccgagaccuc auggagaagg auaaaaaguu cgaccugaga      420

gaaaucuauc uggcuauuca ccauaucgug aaauacaggg ggaauuuucu cgagaaggua      480

cccgccaaaa acuacaaaaa uucgggagca ucaauuggcu ucuuacugga agaggugaac      540

gaucuuuaug gcaauaucau agggaacgag gacgucgcca uuuuggacaa ugauaaauuu      600

gaagacgugg agaagaucau ucugaacgau gaaauccgga auaucgacaa gcaaaagaac      660

guuggacguc uacucgugaa ggauaaaaag gagaaaaaua uugucaccgc uuucagcaag      720

gcaauauuug guuacaaguu caaccuggaa gaccuucugu ugaucgagag ugaugagaaa      780

aacaagcuca cauucaauga cgaaaacauu gaugacaucu uuaaugagcu gucccacucu      840

uuaaacgaua aucagaugga ccugcucacu aaaacccgcg aaauauauuu caaguuuaaa      900

cuuaacauga uugugccuac aggcuacacc aucagcgagu ccaugaucga aaaguaugag      960

augcauaagg cccaccugaa aauguacaag gaguucauua auacuuugaa cgcuaaagac     1020

agaaagaucc uuaaaaaugc cuauucagau uacauuaaca augaaaaggc gaaggcagcc     1080

aacgcucagg agaauuuuua caaaacagua aagaaaacca uaaaggaaaa cgacagugau     1140

acggccaaaa agaucauugg gcugaucgac gagggaaauu ucaugccaaa acagaggacu     1200

ggcgaaaacg guguuauccc ccaccaacuc caucagauug agcuggaucg gauaaucgag     1260

aaccaggcaa aguauuaccc uuggcuugcu gaagagaauc caguggaaaa gaaccgaaaa     1320

uuugccaagu auaaacugga cgaguugguc accuuccgcg ugcccuacua uguggggccu     1380

cucauugaua agacagagag caauaaaaac gaaaaggaga cuaaguuugc guggaugguc     1440

agaaaagcca agggaaccau cacaccaugg aauuucgaaa accugguuga caggaccgag     1500

ucugcaaauc gguuuauuaa acgcaugacg uccaaggaua cuuacaucau aggcgaagac     1560

guguuaccgg cuucaagccu gcuauaugag aaauacaagg uacucaacga gcugaauaac     1620

aucaagguga auaaaaagaa acuugauguc gaacagaagc aacacgugua cuuggaccug     1680

uucacaacca ggaaaaacgu uacuaaggau gaccucgcca caagucugaa uugcgacgug     1740

gagucuauua ccggccuuac ggauaacaag aaauuuaauu ccagccuguc gucuuauauc     1800

gacuugaagg cuauucucgg gaacaucguc gaugacuacu ccaaaaacga agaucuggag     1860

aagauaauug aauauucaac caucuucgag gacggaaaua uuuacaaaga gaaguuaagu     1920

gaaaucagcu ggcugacaga ugagcagaua gaaaaacuau ccaacaucca uuucaagggu     1980

uggggcagac ucucuaagaa acugcuuacu cagauuacca augagaacgg ggaacguauc     2040

auugacacau uguggaauac uagcaacaau uuuauccagg ugauaucaga ugagaguauu     2100

caagccaagc uggcagagau caacggagaa uaugccaaua aauacaaccu cgaggacauc     2160

cuggaugagg cuuauaccag cccccagaau aagaaagcga uucggcaggu aaugaagguu     2220

guggaagaca ucgagaaggc aaugaaaugu gaaccuacau ccauugccau agaguucacc     2280

cgagaaaaga gaaaaucuaa guuaacuaac acgagguaca aaaagaucuc agagacauac     2340

gaaaagauua ccgaugagcu gaucagugag uaugaacuug gcaaauugca aagcgagcuc     2400

gacuccaagg ucaauaacau gcgcgaccgg uacuaucugu acuuuaugca gcuagguaga     2460

gauauguaua cuggggagaa aaugaauauu gacgaacugc accagaagua cgauaucgac     2520

cacauacucc cacagucuuu caucaaagau gacucgcuga acaacagggu gcuuaccagc     2580

aaguccguca auauuaagga aaaaucagau aagacagcug ccgacuugua ugcagccaaa     2640

augggcgauu uuuggcgcaa gcugcgaaaa caaggauuaa ugaccgagca gaaguacaag     2700

aaccuccuga cucggacaga caguaucaau aaauacacga agcagucuuu cauuaaacgu     2760

cagcuugugg agaccagcca aguugugaag cuggcugcca acaucuugca ggauaaauau     2820

agaaauacua agauaauuga aaucagggca cgccucaacu ccgaccugcg gaaaaaguac     2880

gagcuaauca agaauagaga aguaaacgac uaucaucacg cgauugaugg cuaccugaca     2940

accuuugucg ggcaguaucu cuacaaagug uaucccaagc ugaggucaua cuucguuuac     3000

gacgauuuua aaaagcuaga cagcaauuau uugaaacaua uggauaaguu caacuuuaua     3060

uggaagcugg aggacaaaaa ggcugaagau guguacgaca aagucaauga ugaguucgug     3120

uuaaacguac cugagaugaa ggaauauauc cgaaaaauuu acaauuauaa guacaugcuc     3180

gugucuaagg agguuacaac uaaaaacgga gccuuuuaug accagaccaa guacaaugca     3240

aaaacgauca accugauucc aaucaagaaa gauaagccca ccaauauaua cgguggcuau     3300

aaggggaaag ucuccaguua caugaugcuu gugaagaucc agaaaaagaa agaagugauu     3360

uauaaguucg ucggaguucc gcgccugugg acagacgagc uagaucggcu caucgacacu     3420

gacgagaaaa aggcccuguu gaagaaauga                                      3450


<210>  2858
<211>  3459
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2858
augaacgccg agcacggcaa ggaagggcug cucaucaugg aggaaaauuu ccaguacaga       60

auuggacuug acauagguau caccagcgug ggcugggcug uccugcaaaa caauucccag      120

gaugagcccg uuaggauuac agacuugggg gugcggaucu uugauguagc agaaaacccu      180

aaaaauggag acgccuuagc ggcuccacgc cgagaugccc guacuacgag aaggcggcug      240

cgcagaaggc ggcaucgccu agagcgaauu aaguuccucc ugcaggagaa cggccuuauc      300

gaaauggacu cuuuuaugga gagauauuac aaagguaauc ugccggaugu guaucaguug      360

agguacgaag ggcucgaccg gaagcugaaa gaugaggagc uggcacaagu ccucauacac      420

aucgccaagc aucguggauu ccgcucaacc agaaaggcug aaacaaaaga gaaggaaggc      480

ggcgcagugu uaaaagccac uaccgagaac cagaagauua ugcaggaaaa aggguauagg      540

acaguuggag agaugcugua ccuugacgag gcuuuucaca ccgaaugcuu guggaaugag      600

aaggguuaug ugcugacucc ccggaaccga ccugaugacu acaagcauac aauccuacgc      660

aguaugcucg ucgaagaggu gcacgccauu uucgcggcac agagagccca cggcaaucaa      720

aaagcuaccg aagggcugga ggaggccuac guagaaauca ugacgagcca gagguccuuu      780

gauaugggac caggccuuca gcccgacggu aagccuucuc cauaugcaau ggagggguuc      840

ggagaccggg uuggcaaaug uacuuuugaa aaggaugagu accgcgcucc caaagccacc      900

uauacagcgg aacuguucgu ggccuugcag aagauaaacc auacuaaacu cauugacgag      960

uuuggcaccg ggagauucuu uucggaggaa gagaggaaga caaucauugg acuguuacug     1020

ucaagcaagg aacucaaaua cgguaccauc cguaagaaac uuaauaucga uccuagucug     1080

aaguucaacu ccuugaauua uucugcuaaa aaggagggcg agacggaaga ggaacggguc     1140

cuagacacug agaaggcuaa auucgccagc auguuuugga cauacgaaua uuccaagugc     1200

cugaaagauc gaaccgagga gaugccagug ggggaaaagg cugaccucuu cgauagaauu     1260

ggagagauac ugacugccua caaaaacgac gauucaagga guagccgccu uaaggaacug     1320

ggcuugucug gugaggaaau cgacgggcuc cuggauuuau ccccggcaaa guaccaacgg     1380

gugucacuga aagccaugag aaagaugcag cccuaucuag aggacggccu cauuuacgac     1440

aaagcaugug aggcggcagg auaugauuuu agggcccuga acgacggcaa uaagaaacac     1500

cuuuugaagg gggaagagau caacgcuaua gucaaugaua uuacaaaccc uguugugaag     1560

cgcagcguaa gucagaccau caaagugauc aaugccauua uccaaaagua cggaucucca     1620

caggcaguca acauugaacu ggcccgagag auguccaaaa auuuccagga ccggacgaac     1680

cucgaaaagg agaugaaaaa gcgucagcaa gagaaugaaa gagcuaaaca gcagauaauc     1740

gagcugggua agcagaaccc caccggccaa gauauucuua aguauaggcu guggaaugac     1800

caagggggau acugcuugua uagcggcaaa aagaucccuc ucgaggaacu guuugauggu     1860

ggguacgaca ucgaucauau uuuaccauac ucgauaacau ucgacgauuc uuaucgcaac     1920

aaagugcugg uuacugccca ggagaaucgg cagaagggca acagaacccc cuacgaauau     1980

uuuggagcag acgagaaaag gugggaagau uacgaggcgu ccgugcgacu acucguccgc     2040

gacuauaaga agcagcagaa acugcuuaag aaaaauuuca cagaagagga gcggaaggaa     2100

uuuaaagaga gaaacuugaa cgacacuaag uacaucacca gggugguaua uaauaugauu     2160

cgucagaacc uggaacucga gccguucaau cacccugaaa agaaaaagca gguuugggcu     2220

gugaacggcg ccgucacauc auaccugcgc aaacgguggg gguuaaugca aaaggauaga     2280

aguaccgaca ggcaccaugc aauggaugcc guggucaucg cuuguugcac ugacggaaug     2340

auucacaaaa ucagccgaua caugcagggu cgggagcugg ccuauucccg caauuuuaag     2400

uuccccgaug aggaaacggg cgagauacuu aacagagaca auuuuacaag ggaacagugg     2460

gaugagaagu ucggggugaa aguuccauug ccuuggaacu cuuuccggga cgaacucgau     2520

auccgacugc uaaaugagga ccccaagaac uuucugcuca cccaugcaga ugugcaaaga     2580

gagcuggacu acccaggaug gauguauggc gaagaggaaa gccccauuga ggagggucgc     2640

uacaucaauu auauuaggcc ucuuuucgua ucacguaugc caaaccacaa agucacuggg     2700

agugcucaug augccaccau ccgguccgcg agagacuacg aaacaagggg cgugguuaua     2760

accaaggugc cguugacuga ccugaaauua aauaaggaua acgagauuga aggauauuac     2820

gacaaagaua gcgaccgccu ccuguaccag gcccuugucc gacagcuguu gcuccacggc     2880

aaugauggga agaaggcuuu ugcagaggac uuccacaaac ccaaggccga uggaacagaa     2940

ggcccugugg uaagaaaagu gaagaucgag aaaaagcaga cgucuggugu uaugguccgg     3000

ggagggaccg gcauugcugc caacggugag auggugagga ucgacguguu ucgcgaaaac     3060

ggcaaguauu acuucguccc aguuuauacu gcagaugugg ugcguaaagu acugcccaau     3120

agagcggcca cacauaccaa gccuuacuca gaguggcggg ucauggacga ugcuaacuuu     3180

guguucaguc uauauagcag ggaccugaua cacguuaaau ccaagaaaga caucaagaca     3240

aaucucguga acgggggacu gcuuuugcaa aaggaaauuu uugccuacua uacuggcgca     3300

gauaucgcua ccgccucuau ugcaggguuc gccaaugacu cgaacuuuaa auuccgcgga     3360

cugggcaucc agagcuuaga gauauuugaa aagugucagg ucgauauucu cgguaauauc     3420

uccgugguuc gacaugagaa cagacaggag uuccacuga                            3459


<210>  2859
<211>  3462
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2859
augaagaaca uccugggccu cgaccuuggg cugagcucca uuggaugguc ugugauaaga       60

gagaauagcg aagaacagga auuggucgcc auggguagua ggguugugag cuuaaccgcu      120

gcagagcugu ccucuuucac acaaggcaac gggguaucga ucaauucaca gcggacucag      180

aaacgcacgc agcgaaaggg auacgaucgu uaucaacuaa gaaggacccu ccugcggaac      240

aaacuugaca cacugggcau guugcccgau gacagccuca guuaccugcc uaagcugcag      300

cucugggguu uacgcgccaa agcggugacu cagagaauug aacugaauga gcuugggagg      360

gucuugcugc accuaaacca gaagcgggga uauaagucca ucaaaucuga uuuuagcggc      420

gacaagaaaa uuaccgauua cgugaagaca guuaaaaccc gcuaugacga gcucaaggaa      480

augcgacuga cuaucggcga gcuuuucuuu agaaggcuga cagaaaaugc uuucuuucgg      540

ugcaaggagc aaguguaccc acgucaggcc uaugucgagg aguucgauug uauaaugaac      600

ugccagcgca aauuuuaccc ggacaucuug accgaugaga cgauuagaug uaucagggac      660

gaaauuaucu acuaucagcg gccccucaag uccugcaaau accugguguc acgaugugag      720

uucgaaaagc gcuuuuauuu aaaugcagcc gggaaaaaga cugaggcugg accuaaagua      780

aguccaagaa ccagcccccu guuccaaguu ugcaggcucu gggagucuau aaacaauauu      840

guggucaagg aucggcgcaa cgaaaucgug uuuauuuccg cagagcagag agccgcucuu      900

uucgacuuuc ugaauacaca ugaaaaguug aaagguucag accuacugaa gcuccugggc      960

cuuagcaaaa cuuacgggua uaggcuggga gagcaguuca agaccggcau ccaagguaac     1020

aaaacacgug uggaaaucga gcgggccuug gggaauuacc cugauaagaa gcgacuccug     1080

caguucaacu uacaggagga aaguucuucc auggucaaca ccgagacggg agaaauuaua     1140

ccaaugauca gccugucguu ugagcaggaa ccccuauaua gacucuggca cguucuguac     1200

ucuauugacg auagggagca acuucagucc guguugcgcc agaaauucgg caucgacgau     1260

gacgagguac uggaacggcu cucagcgaua gaucugguga aggcaggcuu ugggaauaaa     1320

aguagcaagg ccauuagaag gauccuuccu uuccugcagu ugggaaugaa cuacgcugag     1380

gccugugaag cagcugguua uaaucauucc aacaauuaca cuaaagccga gaacgaggcg     1440

cgcgcccucc uggaccgauu accagcaauc aagaagaaug aacugcggca accggucgug     1500

gagaaaauuc uaaaccagau gguuaaugug gucaacgcuc ucauggaaaa guauggccgu     1560

uuugaugaga ucagagugga acuggccagg gagcuuaaac agucuaagga ggaacgcagc     1620

aauacauaca aaucaauuaa caagaaucag cgggagaacg aacaaauagc uaagagaauc     1680

guagaguaug ggguucccac caggagucgc auucagaaau acaagaugug ggaagagucc     1740

aaacacugcu guaucuauug cggacagccu guggacgucg gcgauuucuu gcgagguuuu     1800

gacguggagg ucgaacauau cauuccaaag agccuguacu ucgacgauuc uuuugccaau     1860

aaaguguguu caugccggag uugcaacaag gagaaaaaca auagaacugc auacgacuau     1920

augaagucaa agggggaaaa agcccucucc gauuacguug agagggugaa cacaauguau     1980

accaauaacc agauaucuaa gacgaaaugg caaaaucugu uaacccccgu agacaagauc     2040

ucgauugauu ucaucgaccg ucagcugcgc gaaagccagu acauugcucg gaaagcgaag     2100

gagauccuua cauccauaug uuauaacguc acugcaaccu caggcagugu gacaucuuuu     2160

cuuagacacg uuuggggaug ggauacugug cuccacgacc ugaauuucga uagguacaag     2220

aaagucggcc uaaccgaggu gaucgaagua aaccaucgag ggagcgugau ucggcgcgag     2280

caaaucaagg acugguccaa aagauuugau cacaggcauc acgccauuga cgcucugaca     2340

aucgccugca ccaagcaggc auauauacag cggcucaaua accugcgagc cgaagaggga     2400

ccugauuuca auaaaauguc acuugagaga uacauucaga gccaaccaca cuucucuguu     2460

gcucaggucc gcgaagccgu ggacaggauc uugguguccu uucgugcagg uaagcgggcg     2520

gucacucccg gcaagagaua cauuaggaaa aaccgcaagc gaaucagugu ucagagcgug     2580

cugauaccga gaggggcuuu aucugaggaa uccguguaug gaguaaucca ugucugggag     2640

aaagacgaac agggccacgu gauucaaaag cagcgggccg uuaugaaaua cccuaucacg     2700

ucaauuaaua gggagaugcu cgauaaggag aagguggucg acaaacgcau ccaucguaua     2760

cugaguggua gacuugcaca guauaacgau aaucccaagg aagccuucgc uaaaccagug     2820

uacauugaca aggagugucg gaucccuauc aggacaguuc gcugcuuugc caaacccgca     2880

auuaacaccc uggugccauu gaagaaagau gacaagggga accccguagc uugggucaau     2940

ccuggcaaca aucaccaugu ggccaucuau cgagaugaag acggaaagua caaagagaga     3000

acugugaccu ucugggaagc ggucgaucgg uguaggguug gcauuccagc cauagugaca     3060

cagccggaca ccaucuggga uaacauucuc caacgcaaug acaucagcga gaacguacug     3120

gagucgcuac ccgaugugaa guggcaguuu guccuguccc uccagcaaaa ugaaauguuc     3180

auucugggga ugaacgagga agacuauaga uacgcuaugg accagcagga uuacgcacuu     3240

uugaauaaau aucuguacag ggugcagaag uuaucuaaaa gcgacuauuc auuucgguac     3300

cacacugaga caaguguuga agauaaguau gacggaaagc cuaaccucaa acuguccaug     3360

caaaugggua aguugaaacg ugugucuauc aagagccugc uaggccucaa uccacacaaa     3420

guccauauau ccgugcuggg ggagaucaag gagauuucau ga                        3462


<210>  2860
<211>  3474
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2860
augaacuacu auaauggcuu caucauuacc gagaacgcca ugacaagcaa gcaggaaaaa       60

cacggggcuc ugacuuuugg acucgacaua gguaucgcau ccgugggcug ggccguccuu      120

ggggagucua gaauuguuga ucugggagug aggugcuucg acaaggcgga aacggcuaaa      180

gagggcgaau cauugaauuu aacccggcgc auggcccgac ugcuacguag aaggcuccgg      240

cgcagagcau ggaggcugac aaaacuugcc cggcuguuga aacgcgaggg ucucaucgcu      300

gaugcaaacc uguuuaagca uaagauugag aaaaauacuc ccguaccuaa gaguagcugg      360

caacugcgag uggacgggcu cgauagacag uuaaacgccg aagagugggc uagggucauc      420

uaccaccugu guaaacaucg gggauuccac uggauauccc gugccgaaga gaaggcggca      480

gagggcgacu cuaaaggcga agggggaaag gugaagcagg gucuugccgg caccaaaaag      540

uugaugcagg agaaaucgua ucgcacagcu gccgaaaugg uucuggcaga guuuccagau      600

gcucaaagaa auaagcaggg ggacuacuca aaagcccuaa gcaggauccu ccugggagau      660

gaacuuaagg cgcuguucga gcaucagcgg aaauugggca acccgguggc caccgagaag      720

cucgaagcag cuauucuggg uaauggggac aagaaaugcg gauuauuuug ggcccagaac      780

cccgcucugg ccggcaauaa gcuccuugag augcugggcc gauguacuuu cgaaaagaca      840

gggggagaug acgguaaggg cgaguaucgc gcaccuaaag ccaguuuuuc cgcugaaaga      900

cacgucuggu ugacccaccu aaacaaucug aggaucgugg uagaugggac gacucggcca      960

cucaacgagg cggagcgcag aguugcacug cccaugccuu accaacaggc cggagaccuu     1020

ucuuauaagc agcugggcgg ugcuuugauu aaggaagggu uccuccagaa agacggcuuu     1080

aaguucgccg gacugccaua ccccaccaaa caagcaagcg aggaaaaggc caaagauccu     1140

gagacauccg uguuaugcaa gcugccagcu uggcauacuc uaaggaagac ccucgaaaaa     1200

cacggccugg agucagagug gcagaaacuu aguacagacg ccuuggaugg gaagccgcag     1260

cugcucgaca auaucgcaug gguccugagc guguacaagg augacgauga agugaaaacc     1320

gagcuuggaa agcugaacuu gcccaacacg gaaaagauga uagacgcgcu ccuggauauu     1380

cguuuugaca aauucucuaa uuuaucccug aaggcucuau caaaaaucgu cccuuucaug     1440

gagcaagguc agcgguauga ugaggccguu gcaaacauuc cagaauacgg ccaucacagc     1500

cagcuccaca aggugggggc cggagagcau aaauaucugc ccccuuuuua caaggaacga     1560

gacaaaacug gcagaauggu auucaaugac gauauggaca ucccaaggaa ccccgugguc     1620

cuucgcgcuu ugaaucaggc ccggaaggug guuaacgcac ugaucaagga guaugguagu     1680

ccgcacgaag ugcauauuga gauggcuaga gaucucucua ggccucugga cgagcgccga     1740

gauauagaaa aauuacaaaa ggaguuuggg gaacggaaua aaaaggacaa agagcuguuc     1800

gcccaggauu uuaacaucgu cggcacagug aagggaaagc aguucgaaaa auaccagcuu     1860

uaucgugagc aacagggcaa guguauuuac cccuccaugg acgagaaaca ggggaucugc     1920

uguuacagcu ugaagccacu cgaucugcac agaguacuau augaagaggg auacgcggaa     1980

auagaccaug cccugccuua uucgaggucu uuugaugacu ccaaaaauaa ccgcguucuc     2040

gugcuggcua aggagaaucg ggauaagggu aacagaaccc ccuucgaaua ccuuggcgac     2100

aaagacaauu cagagccaug gaggcaguuu gucgcauucg uggaggggaa caaggccuau     2160

cgauuggcua aacgcagucg gcuguuaaga aaggauuuug gagacaaaga agccaaggag     2220

uucagggaac guaaucucaa cgauacucgc uacauuugca aguuuuucaa aaacuauguc     2280

gagcaauacc ugcagcuuca cgaggacagc gaagcaaagc gguguguggu ucuguccggc     2340

cagaugacau cuuucuugag agcgaggugg ggucucguga aaguacgagg ggauagcgac     2400

cggcaccaug cccuggaugc ugccgucgug gcagcuugcu cacacggcau gguuaagcgc     2460

cuaagugacu acuccagaag gaaagagcug gaacaggugc gggagggauu ugucgaugug     2520

gacaccggcg aaaucguaaa ucccgccaug uuccaacagg ugcaggagca uuuuccuaac     2580

ccauggccgc acuuccgaga ugagguuaag cucagacuga gcaucgacga ucccacgcuu     2640

uugcgcucug aaauggagaa auuugggacc uauacaccug aaucacugga ggcauuaagg     2700

ccacucuucg ucagucgugc uccccaacgg agaaauagcg gagccgcgca caaggacacu     2760

auuuacgccc agccuaagag gcugguggca cagggcggug ugacccagaa agucgcucuu     2820

uccucucuga cacuaaagga ccucgauaaa cugaucgacc cacaucgcaa cgaacgauug     2880

uaugccgcca uuagagcucg gcuggagcaa cacgggggaa agggcgauaa agcauuuccc     2940

gccucgaauc cguuaaggaa gccugacagc gaggguaacc caacuggccc cauaguucgc     3000

ggggugacca uggugaucga uaagcucucc ggcauuccua uccguggagg gaucgcgaaa     3060

aaugacucaa ugguaagagu cgauguguuc acaaaggcua acaaauuuca ucugguuccc     3120

guguacgucc accauagugu gcuuggacug ccaaaucagg ccauuguugg cgguaaggaa     3180

gagucugaau ggacccagau agacgauagc uucgacuggc acuuuuccau cuauccuaac     3240

gauuugauuc gggugacucu caaacaggag gggagguucg gauacuaugc aggcugugac     3300

cgcucaacgg ccgcuaucca ccugugggca caugaccgaa aucaacaggu agguaaggau     3360

ggccuaauua gaagcaucgg ggucaagaca gcccugucua uagaaaaauu uaacguggac     3420

gugcuuggaa aucgguaccc cgcgccaccg gagaagaggc gcgaucucgc uuga           3474


<210>  2861
<211>  3486
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2861
augaagaaaa uccugggccu cgaccuuggg agcgccucca uuggaugggc ucugguggag       60

gaaaccgagc agaugcaaua caacauaguc ucuuuggguu caagaaucau ucccuauaau      120

ggcacagaag ggcaggaguu cgaaaaggga acuggcgaga guaggaacag cuuacggacg      180

aaagcacgca ccgcccgaaa ggguuacgau cguuaucagc ugagaaggaa aaaucuagag      240

aagauccucc ugcagaacgg gauguacccu aaugaacaag uucgggcgcu ucagaagcug      300

gaguugugga aacuccgcgc ugacgcccca aacgugcaga uuucccugcc ggaacuggga      360

agacucuuau uuuggcugaa ucagaagagg ggcuauaaau cuucgcgguc agaugcaaac      420

cuugacaaga aagauacaga guacguagcc acugucaaga gccgccacca aaagaucaaa      480

gacgcuggcc agaccguggg gcaguauuuc uacggacaga uauuggagaa ugaauaccau      540

cgaaucaagg agcaaguguu ucccagagaa gcauauauug aggaguucga ugccaucguu      600

gcuaggcaga aagaauacua uccuacaauu cugaagcagg agcuaaucga caaaauacgg      660

aacgaaguga uuuacuuuca acguggucuc aagagucaga aaggccuggu cuccgugugc      720

gaguucgaag ggcgcgaguu uaauggacac uucggcgguc caaagguagc ccccaagucu      780

agcccuaucu uucagcuugg gaaaauuugg gaaaacauca auaacaucaa gauugcggau      840

auagagcuga ccgcagagca gaaauugguu uucuuuaauu aucucaacug ucaugaaaga      900

aucacugagg ccaagauuag ggaacuguua aaacugucca aggacucacg gcucaacgga      960

caacuuaaga auggccugac aggcaacaaa accuacaguc agaucgcuag cugcuucggg     1020

aauaagauag gagaguauga acgauuguuu aaauucgagc uagaggugau ugaaucugag     1080

aagcuggaau acacguacca cccaaaaucc cauauccccg ucggugagaa gcgcagaaag     1140

uauaucucag agaacgugga aaaagagccu uacuauaggc ucuggcacac uauuuacagc     1200

aucaaggaua aagacgaaug ucgggccgca cugauuaaga auuucggcau agaggaagag     1260

aucgcugaga aacuugccag ucuggauuuu ucugccgggg gauucggcaa caagugcgcg     1320

aaggugauuc gcaaaaucuu gccauaucuc auggaaggug acaaguacaa ugucgcaaug     1380

gacuaugcug gguacaacca cuccuuuagc cagaccaaag augagaaucu gcagagaguu     1440

uuacuggacu cgaucaacca ucuaccgaaa aauucucuca ggcaacccau uguggaaaag     1500

auacugaacc agaugaucaa ucuuguaaac gaggugauua agcagugggg ccguccugau     1560

gaaauccggg ucgaguuggc ccgaaaacug aagcagucca agacagagag aaaugacgau     1620

gacaacuuca ugaggaaacg cgaacgggag aacgaaauua ucaaggcuga ucucgccaaa     1680

uacggacuga aggcaacuag aaauaacgug auaaaaugga ggcuuuauca cgagaucgac     1740

gauaaggaca agaaacugaa ugcccgcugu auuuacugcg gccaaccaau cucauugacc     1800

gaagcuauuc ucgggaacaa uguugaugug gagcauauca uacccaagga caaacuguuu     1860

gacgauaguc agagcaacaa gacauuagcg caccgauccu guaauucugg aaaagguaac     1920

aagaccgcau augacuucau gaaggauaaa ccucaguuug aggaauacgu cgcccgggug     1980

gaggaacugu auaagaaaca ccuaauuagc cucacgaaga aacguaaguu ccugauguca     2040

gaggacaaga ucccaaguga uuuuauugac agacagcuua gggaguccca auacaucgcu     2100

cgcaaaagcc gggaaauauu gcagacugua ugcagaaaug uuuggucuac agauggcggg     2160

gugaccgccc aucugaggaa gcucugggga ugggacgaug ucacuaugaa ccugcagaug     2220

cccaaauaua agggcaucgg uaugacagag auuguggaau gggagucaaa ucacgggaaa     2280

aaccaacaua cccaggaagu caucaaggac uggaguaaac gagaugacca ccgccaucac     2340

gcaauugaug ccuuaguggu ugcuuguacg cggcagggcu acauccagag aauaaauacc     2400

cugaacagcu ccgagacaaa ggacgagaug cacaggaagg uggccggauc ucuuguagag     2460

uucaaucaaa aacauacuuu gcucaacaag uaccugauua ccgaaaaacc guuuacaacu     2520

ucggaagucg aggacguggu uagcaagauc cuaguguccu ucaaagcagg caagaaggcg     2580

gcuaccccug ggaaacguaa ggucggaaaa cgcgguaaca aacagguggu acaggauggc     2640

aucauuaucc ccagaggggc ccugcacgca gacucaguga guggaucuau uaaacaguau     2700

gauccaacag gcaauuuuga aaaccauaau guuauaaagu acaaggucgg uguggggaaa     2760

ggcuaucucu ucgacggaaa ggaugacaaa aagaaaaucg auaagaccau ugacugcauc     2820

guggauaaga aaauucggga ccugaucaag caaagggccg aggagagcaa cuacucccuu     2880

aaaaagaugu ucgaaacucc uauauuguuu aaucagcgag cugucuucuc aguucggugu     2940

aaaacgggca acaaggccgu ggugcccauc cgcaaugggu auguaguccc agauaacaau     3000

caccacacag caauuuacaa guaugacgga ggccugaaag agcagugcgu gagcuuaaug     3060

caggcuaucg aaagaaagag guacaaccuc cccauuauca uaaagaaucc uaaggagauu     3120

uacgauaaaa ucaccgaaau cgccaacauu aaucaacugc uuuuugacuc ucugccaaag     3180

uuggacuggg aguucguuac uacccuccag aaaaacgaua uguuuauacu ggguauggac     3240

uauuccgcga cagaggaagc ccuagcugau gggaauuacc ggcugcucag ugaguaucug     3300

uacaaggugc agaacaucag cgacggaauu uaucgacuuu gccaucacac caucacuaag     3360

uucgauuugu cuaaagcaaa caagccggac aaaagauuuu acaauauuca aucccugaag     3420

gccuggaaca cauuaaaucc ccagaaaguc aagaucaaca uacucggcca gaucgaacgc     3480

caguga                                                                3486


<210>  2862
<211>  3501
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2862
augcacgugg agaucgacuu cccccauuuu agcagaggcg auucccaccu ggccaugaac       60

aagaaugaaa uucucagggg gucuucaguc cuuuaccggc ugggauugga cuuagguagu      120

aacagccugg gcugguucgu uacccaucua gagaaacgcg gggaucgaca cgaaccugug      180

gcucucggac caggcggugu acguauauuu ccggacggga gagaucccca guccggaaca      240

ucuaaugcag uggacaggcg gauggcccgc ggcgcgagaa agaggcggga ucgcuucguc      300

gagcgaagaa aagaacugau cgcugcccuu auuaaguaua accuguugcc ugacgaugca      360

agggagcggc gugcccucga ggugcuggac ccauacgcuc ugcgcaaaac ugcacucacg      420

gauaccuuac ccgcccauca cguuggcaga gcucuguuuc accuuaauca aaggcggggg      480

uuccagucga accgaaagac agacucaaag cagagcgaag auggagccau caaacaggcg      540

gcaagucgcu uggccacuga caaggguaau gagacccugg gcguguuuuu cgcugacaug      600

caucuaagaa aauccuauga agauaggcaa acagccauuc gggcagagcu cguccgccug      660

gggaaggacc accuuaccgg aaacgcuagg aaaaagaucu gggccaaggu gagaaaacgu      720

cuguuuggcg augagguauu gccucgggcg gacgccccac augguguucg agcaagagcu      780

acuauaacag ggaccaaggc cucuuacgau uauuacccca cgagggacau gcuccgcgau      840

gaguucaaug cuaucugggc cggacagagc gcacaccacg ccacuauuac cgacgaggcu      900

cggacagaaa ucgagcauau uaucuuuuau cagagaccuc ugaaaccagc gauagugggc      960

aagugcacuu uagauccggc aaccaggccc uucaaagaag acccugaggg cuaccgcgcc     1020

ccaugguccc acccccuggc ucagcgauuu cggauucucu cagaggcccg uaaccuugaa     1080

aucagagaua cagggaaggg aaguaggcgc cugaccaaag agcaaagcga cuuggucgug     1140

gcagcccuac uggcuaaucg ggaagugaag uucgacaagc ucagaacgcu gcuuaaacug     1200

ccugccgagg caagguucaa cuuggaaucu gaucgacgcg cggcucucga cggugaucag     1260

acugccgcac ggcuguccga caagaaaggc uuuaauaagg ccuggagagg guucccaccc     1320

gagaggcaga uugcuaucgu cgcccguuua gaggaaacag aggaugaaaa cgagcugauc     1380

gcauggcuag aaaaagagug ugcucucgac ggagccgcgg ccgcucgcgu ugcaaauacc     1440

acucugccgg auggccauug ccggcuuggu uugagagcca uuaagaagau agugccuauc     1500

augcaggacg ggcuggauga ggacggcgua gcuggagccg gcuaccacau ugcagcgaaa     1560

agggccgggu augaucaugc uaagcucccc acaggagaac aacugggucg acuuccauac     1620

uauggccagu ggcugcagga cgccgugguc gggucaggag augcacggga ccaaaaagag     1680

aagcaguacg gccaguuucc uaaccccacc gugcacaucg guuuggggca gcuccgcaga     1740

guugugaacg accugauaga uaaauauggc ccacccacgg aaauuagcau cgaguucacc     1800

agggcuuuaa agcugaguga gcaacagaag gccgaacggc agcgagagca gagacgcaau     1860

caagacaaaa acaaggcaag ggccgaagaa cuagcuaaau uuggacgucc ugcgaaucca     1920

cggaaccucc ugaagaugag acuuugggaa gaguuggccc acgauccgcu ggacaggaaa     1980

ugugucuaca caggcgagca gaucucuauu gaacgccucc uguccgauga gguggacauc     2040

gaucauauuu uacccguagc aaugacucug gacgauagcc cugcuaauaa gauaaucugc     2100

augcgauaug ccaacagaca caagcggaaa cagaccccau cggaagccuu cgggucuucc     2160

cccacacuuc agggacauag guacaauugg gacgauauug cugcacgcgc cacugguuug     2220

ccucguaaca agagauggcg guuugacgcg aaugcuaggg aggaguucga uaaacgcggc     2280

ggguuucucg cccgacaacu gaacgagacc ggauggcuag caagacuggc caagcaguac     2340

cucggcgcug uuacagaccc aaaucagauc uggguggucc ccggucggcu gaccucaaug     2400

cuuaggggga aauggggcuu gaacggacug uuaccgagug acaauuaugc aggcgugcag     2460

gauaaggccg aggaguuccu cgcgagcacu gacgauaugg aauuuuccgg ggucaaaaac     2520

cgcgcugacc acagacacca ugccaucgau ggacugguga cggcacuuac agacaggucu     2580

cuguugugga agauggccaa ugcuuacgau gaagaacacg aaaaguucgu uauugagccu     2640

ccauggccca ccaugcggga cgaucucaaa gccgcacugg agaagauggu gguaagccau     2700

aaaccugacc acggcauaga agguaagcua caugaggauu cagcuuaugg guuugucaaa     2760

ccccuggacg ccacuggacu caaggaagag gaggcgggca accuggugua ccguaaggcc     2820

aucgaaaguc uuaacgagaa ugaaguugac cgcauucgag auauccaauu gagaaccauu     2880

gugagggacc acgucaacgu ggagaaaaca aaggguguag cacuggcuga ugccuuacgg     2940

cagcuccagg cuccauccga cgauuauccu caauucaaac acggccugcg ccaugugaga     3000

auccuuaaga aagaaaaggg ggacuaccug guucccauag caaauagggc cagcggaguc     3060

gccuauaagg cuuacucugc gggcgagaac uucugugugg agguguuuga aaccgcaggg     3120

ggaaaauggg auggcgaggc cguccggcga uucgacgcua auaagaaaaa cgccggucca     3180

aagaucgcac acgccccgca guggagagau gcuaaugaag gggcgaagcu aguuaugcgc     3240

auucauaaag gagaccucau caggcuggau cacgagggcc gggcccguau uaugguggug     3300

cauagauugg acgcagcugc cgguagguuu aagcuggcag aucacaacga aacuggcaau     3360

uuagacaaac gccacgccac aaacaaugac aucgauccuu uccgguggcu cauggcuuca     3420

uacaacacgc ugaagaaacu ugccgcggua cccguccgag uggacgagcu ggggagaguu     3480

uggaggguga ugccaaauug a                                               3501


<210>  2863
<211>  3507
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2863
auggccagac ccgcuuucag ggcaccucgg cgcgagcacg ugaacggcug gaccccagac       60

ccgcaucgaa ucagcaagcc cuuuuucauu cuggucuccu ggcaccuccu uucucguguu      120

gugauagauu caaguagcgg gugcuuuccu ggaacaucca gagaccauac ugauaaauuc      180

gccgaauggg agugugcggu acagccauac aggcugucuu uugacuuggg uaccaauucg      240

aucggcuggg gguuacugaa ccuagaucgg caaggaaagc cccgcgaaau uagagcucuc      300

ggcucaagga ucuucagcga cggucgggau ccucaggaca aagccagucu ggcaguggcc      360

cgccgacuug cuagacagau gaggcggcgu cgcgauagau aucugacgag gcggacacga      420

uugauggggg cacucguccg cuuuggacug augccagccg accccgcugc cagaaagagg      480

cuggaggugg cgguugaucc uuaccucgca cgggaacgcg ccacuagaga gagguuagag      540

ccauucgaaa uuggccgugc ucuguuucac cuuaaucagc ggcgaggcua uaaaccggug      600

agaaccgcca caaagcccga cgaggaagca gggaagguca aagaggcugu ggagagguug      660

gaagccgcga ucgccgcagc uggagccccu acccugggug cuugguucgc cuggcgcaag      720

acucggggcg agacacuaag agcaaggcuc gccgggaaag gaaaggaagc ugcguaccca      780

uuuuaucccg cacgccgaau gcuggaggcc gaguucgaua cccuuugggc ugagcaagcc      840

cggcaucacc cugaccuguu gacggcagag gcccgugaaa uacucagaca caggaucuuu      900

caucagcgcc cacugaaacc cccgccugua ggccggugca cuuuauaccc cgacgauggu      960

agagcuccaa gggcccugcc uuccgcacag cgacuccgcc uuuuccagga gcuggcgucu     1020

uugcggguua uucaccuaga ccugagcgaa agaccccuca ccccagcuga gagggaucgu     1080

aucguggccu ucguccaagg gcggcccccu aaggcaggac gcaagccagg caaagugcag     1140

aaguccgugc cguuugaaaa acugagaggc cuucuggagu ugcccccugg gacaggauuc     1200

ucacucgaga gugacaagag gccagaacug uuaggugaug agacuggcgc ccgaauugcu     1260

cccgccuuug ggccuggaug gaccgcacug ccacuagaag aacaggacgc ucucgucgaa     1320

cugcuuuuga cagaggccga gcccgaacgg gcgaucgccg cucugaccgc acgcugggcc     1380

cucgaugagg cuacggccgc aaaacuggcg ggcgccacuc uuccggacuu ccaugguaga     1440

uaugggaggc gggcuguugc cgaacuguug ccugugcucg agcgagagac aagaggcgau     1500

ccagacggac gcguaaggcc cauacgucug gaugaagcag ugaaguuacu gcggggcggg     1560

aaagaccaca gcgacuuuuc uagagaggga gcucuacucg augcccugcc uuacuacggu     1620

gcaguccuug aaaggcacgu ggccuucggc accgggaacc ccgcugaccc agaggaaaag     1680

cgcguuggac gaguggcgaa uccuacuguc cauauugccu ugaaccagcu gagacaccuc     1740

gugaaugcaa uccuggcucg gcauggcagg cccgaggaga uuguaaucga auuagcccgc     1800

gaucugaagc guuccgccga ggacagacgg agggaagaua aacgccaagc ugacaaccag     1860

aagcgaaaug aggaaagaaa acggcuuauc uugucacucg gugagaggcc aacaccgcgc     1920

aaccugcuaa agcugagacu cugggaggaa caggggccug uugagaauag gcgguguccc     1980

uauagcggcg aaaccauuag uaugcguaug cugcuuucug agcaagugga uauagaccac     2040

aucuugccau uuuccgucag ccuggaugac ucggcagcca acaaaguggu cugcuuacgc     2100

gaagcgaauc gaauuaagag aaacaggucu cccugggagg cuuucggaca ugauuccgag     2160

cggugggccg gcauccucgc acgcgccgaa gcucugccua agaauaaaag auggagguuu     2220

gcaccagacg cccuugagaa gcuggaaggg gagggagguu ugcgggcgcg acaccucaac     2280

gauacgagac accugucacg ccuagcugug gaguaccuga gguguguuug ccccaaagug     2340

cggguaaguc cuggccgucu caccgcccug cuuagaaggc gcugggggau agacgcaauu     2400

uuggccgaag cugacggacc gccacccgag gucccugccg aaacacugga uccaagcccc     2460

gcagagaaga accgggcuga ccaucgacac caugccuuag augcgguggu uaucggcugu     2520

aucgacagau ccauggugca gaggguccag cucgccgcag cuucugccga acgcgaggcu     2580

gcagcccggg aggauaauau uagacgugug cuggaagguu ucaaagagga accuugggac     2640

ggguuuaggg ccgagcuuga acggcgcgcu cgaacuaucg uagugagcca cagaccggag     2700

cacggcauug gaggcgcgcu gcauaaggag accgcauaug ggcccguuga uccaccugaa     2760

gagggauuca accuagucgu gaggaagccc auagacggcc ucucaaaaga ugaaaucaau     2820

agugugcggg acccacgccu gagaagggcc uugauugauc gacuggcuau ccggcguaga     2880

gacgccaacg auccugcaac agccuuagcu aaggcggccg aggaccucgc agcucagccc     2940

gccucccgcg guaucaggcg ggucagaguu cugaaaaagg aaagcaaucc aauucgcgug     3000

gagcacgggg gaaacccguc uggcccuagg ucagguggcc ccuuucauaa acuucuguug     3060

gcaggggagg ugcaccaugu agacgucgcc cuccgagcug auggacggag augggugggc     3120

cacuggguua cucuguucga agcccacggg ggaaggggcg cggacggugc agccgcucca     3180

ccccgccuag gggauggaga gcguuuccug augcggcuuc auaagggcga cugccucaag     3240

cuggaacaca aagguagagu gagggucaug caagugguua aguuggagcc uaguagcaau     3300

uccgugguag ucguggagcc acaucaggug aaaaccgauc gcucuaagca cgucaaaaua     3360

ucgugugacc agcugcgagc ccggggcgcu agaaggguua caguggaucc cuuagggcgc     3420

guaagagugc augcaccugg agcccggguc ggcaucgggg gagacgcugg caggaccgcc     3480

auggaaccgg cagaggauau uagcuga                                         3507


<210>  2864
<211>  3513
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2864
augagcaagu acaacguggg ccuggacauc gggauugccu ccgucggaug gucuauaguu       60

gaugcugaca aaaagaaaau cauugaucuc gguucaagaa ucuucaguag cggcaaugca      120

gccgcgaacc aggagaggcg gggguuucgc ggaacccgac gucuuauuag aaggcggaag      180

aaucgccuga cagacgugau caacuucuuu ggcgaaaaga aguucauaga uacuaaggua      240

guggacaaaa auggucacgu ccauuauaag uuuuccguga acaauacgga gucucccuac      300

gaauugagag uuaaaggguu aucggagauc cugaccaagc aagagcuagu gauugcucuc      360

uaucacaucg ucaaacauag gggaauuuca uacgaucugg gcgaccuuga agaugacggc      420

acaagcgggg ugacugauua uaagaccagu aucaacauaa aucggcagcu guugaacgag      480

aagacaguag gacagauuca gcucgaacgc cugaaugagu ucgguaaagu ucgaggccaa      540

guggaagaga acgagaaugu cacccugcuc aacguguuuc cuuccgccgc auacgugaag      600

gaagccacuc agaucuuaac aaaacagaga gaguucuauc cagaaauuac cgacgauuuu      660

aucgcuaagu ucaugcuguu uaucacgagg aaacgggacu acuucgucgg gccgggaaau      720

gagaagucuc guacugacua cggcauuuau aaaaaggaug gucgcacccu ugacaacuug      780

uuugaugaac ugauagggau cgacaagauu aacggagaga aaagagcaag cgccuccuca      840

cuaacagcuc agaucuacaa uaugcucaac gaucugaaua accuuacugu ucccaauacc      900

gaggacggca ggcugacaau acaaaccaag aaagaaauuu uggaggccgc gaagacgacu      960

aacggccucu ucgggaucgc acagaucugc aaaauuaucg gauguaagaa ggaugacauu     1020

aaagguuucc ggauagauaa gagugagaaa aagaucaugc acacacugag cggcuaucga     1080

aaauuucgca aggaauuagc cgacgugaau auugaagcua acgaucugaa uaucaaccuc     1140

guaaauaaga ucgccgacau ucuuacccug aacacugaga aagcagagau aagaaagcag     1200

uuggacaaau cuaauaugga uaucuccaac gaaaugaagg agauugugau caauaaaaac     1260

aaggaguucc uagucgacgg gagggcuaca uggcauucau uuagcuacaa gacccugaac     1320

cuccugauuc cugaacuucu gaauacgcca gaggaacaga ugaccaucuu gacacggcuc     1380

ggacugguga aacccaacaa uaagaaauau gagggcuuaa agaacauacc ugcccgcaga     1440

aucacugaag agauuuacaa uccaguugug gcgagggcca cccgugaagc agucaacgug     1500

uucaaugcua ucacuaaaaa gguaggucgg gauaacauua aagacguuau cauagagcug     1560

ccccgagagg auaaugaagu ggacauuaag aaggggaucg ucaaaagaca aaagcagaac     1620

gagguggaaa aagagacugc cgaugcucua gucaagcagc acaugaccau uagugacucu     1680

gcccucaucg cacaauauag gaaaauacgc ggccuguccc agaaggugcg guacugguac     1740

cagcaggaua caaucugccc uuauugugga aagaaaauug aagccguuca acuuaucaau     1800

aacaaugaca gcuuugaggu ggaucacauu guaccaaucu cggucucuuu cgacgauucc     1860

cagaacaaua agguguuggu ucauucacag ugcaaccagg cuaaagagaa gcaaaccccg     1920

cugggcuggc ucaacaaugg gggagguuuu ggccagagua aagcggacua cauagcaaga     1980

gucaagagca acaagaauua ugccaaaaac aagauugaca aucugcuuaa cgaaacugau     2040

cugaaugaca ucgcuacgca gaggggguuc auccagcgca acuugaauga uacacgauac     2100

gccucccgga uugugcucaa cgaguuuuau ucuuucuuuc guagcaauaa ccugucaacc     2160

aaagugaagg uagugagagg aaaauggacu ucacaaauga ggaagaagug gaauggcguu     2220

gguggguuau ccaaaacccg cgacacauac caccaucacg caaucgaugc cuguauuaua     2280

gcuugcuucc cccugcuaaa ggccuuugac aaagcaauca agcucauuga ugucgacggc     2340

gaaaccggag agauccugca ggauaaaaag gcgauuaaac uugcucagga agccgagauc     2400

guggagaaga uaagcgugau caagaacaaa aaguucgaaa auauggucaa cgagauuuau     2460

aacuucccuu uguuuaaaca gguugaacug gcaaaugaca ucacuaaccu cgagaaucca     2520

gugaaguucu cucaccaugu ggauaaaaag gccaaccggg cuguagccaa ucaaacaauu     2580

uacggcacga gaaucaagga aaaagagguc accaagaggg ggaaaaagga gaaaauacag     2640

gaacgauaca cucugggaac aauuaagaac aucuaugaca ucgaugguuu uaugaauuuc     2700

aagaaauuau acgacaagau uaguaaaaac gacgauguga aguuucugau gcagaaaaau     2760

gaccccaaga ccugggagaa acuuauagau aucuucaaca guuauccuga cagcgaagag     2820

guuacacaag uggauggcaa agucaagcgc guggcaguuu ccccauuuaa auuguacuau     2880

cgggagaaug gguucauuac uaaguacucu aaacacaacg acggacccaa ggugguaucg     2940

aucaaguauu acgauagcaa agucggcucc cauauugaca ucaccccgaa ggaugcuaga     3000

aauaaaguga uacuccaguc acugaagccu uggaggacgg acguguacuu uaacaguauc     3060

acccagcagu augaacuacu gggucucaaa uacucugauc ugaaguucac aaaugggguc     3120

uauggcauua cucgugacgc cuacgagaaa cuuaagcacg gaguuagcga agacggcgug     3180

accgcgugga agcccaucuc caaaaacuca gaguuucgcu ucagcuugua ucggaaugau     3240

agaguaaggg ugauugacga aaagggggau ucuaucgagc uguuauuugc cucccgaaca     3300

acaaguaacg agggauacgu cgaacucaaa ccaauauaua aggcuaaauu cgacagcaag     3360

gaggaagugg gcuucuacgg ucugguuucu aacgggcggc uuauuaagaa aauguccaag     3420

ggaggcuaca aacuguauaa ggugaauacc gauaucuugg guaaaccuua cuaugucacg     3480

cgcgagucag acgaacccaa gcucagucug uga                                  3513


<210>  2865
<211>  3516
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2865
auggccaagu acagcguggg ccuggacauc gggauuuccu cugucggaug ggcuauaauc       60

gauauugaga ccaaacagau cguuaaccuc gguucaagaa uuuuccccag uggcaaugca      120

gccgggaacc aagaaaggcg gggauuucgc ggcauccgac gucuuauaag aaggcggaag      180

aaucgccuga cagacuugaa aaaguuacug ggugagacuu ucuuuagcga ucucaaaaag      240

aucgacucca acggguauga auacuaugau auugacuucc agaucaaugc gaacccuuac      300

gagcuaagag ugaagggaau uacggaaaaa cugucuaaga aagagcuugc uguagugcug      360

caccauauca uaaagcacag gggcauuucg uaugauuugg gcgaccucga ggaugacggg      420

accucaggag ucagcgauua caaaaauagu aucaacauua aucggcagcu gcugcgcgaa      480

aagacagugg gucagaucca acucgagcga uuaaucgaau uuaaccaggu ucauggccag      540

gugaagaaag ccgaggacgu cucccugcuu aauguguucc caucuagcgc auauagagau      600

gaggccaaca agauuaugau aaaacagcaa gaauuuuacu ccgagaucac uaauucauuc      660

auggaacagg uagaccaguu gguuaguagg aagcgggacu acuuuauugg gccgggaaac      720

gagaaaagcc guaccgauua uggcaucuac aagucugacg gucgcacacu ggauaaucua      780

uucgcugaac ucauagggac cgacaaaauu aacggagaga agagagcauc cgccucaagc      840

cugacugcuc agguguauaa ccuucugaau gauuugaaca aucuccacgu caguaacaca      900

gaggacggca agcugaccac gucuacuaaa aaggaaauca ucaaagccgc gaagaccaca      960

gauggcgugu uugggauuuc ccaaaucugc aaaauuauag gauguaagaa ggaggaaauc     1020

acugguuuca ggcaggacaa aagcgagaag aaagugauuc auaccuuagc aggcuaccgg     1080

aagauccgaa aagcccuggc uacaaucaau auugaagcca acgagcucaa ucccaccguc     1140

uuugaucaga uagcagacau ccuuacgcug aacacugaga aggcugaaau ucgcaagcaa     1200

uuggauaaag uucagcuaga caucacagac gagaugaagg aacuggugau ugauaaaaau     1260

aaggacuucc ucguagaugg gagagccacc uggcacucgu ucucuuauca gacucugaac     1320

augcuuaucc cugagcuguu gaauacaucc gaagaacaga ugaccauacu cacgaggaug     1380

ggacugauga aaucagacaa cgagaaguac cggggcuuaa agaaaauccc agcggaaaag     1440

auugccagug agaucuuuaa ucccgugguc gcaaaaagca cccgcgaagc uauuaaggug     1500

uucaacgcca ucuccaaaga gauagguaag aauaacauug agcacguugu gaucgaacug     1560

ccuagagaug acaacgagga agaugagaaa aggaagauuc aaaagcuaca gaaagcuaau     1620

gaaacagaga aggaggccgc agacaaccuc gucaaacaua agcuggggau cucugauagc     1680

gcccuuauag cucacuaucg uaaauuuaag ggcuugucac agaaggugcg guacugguac     1740

cagcaagaaa cuuauugccc auacugugga aaaaucauug aggcgaccca gcugaucaau     1800

aacaaugaca acuucgaagu agaucauauu auccccauaa guguuuccua ugacgauagc     1860

cagaauaaca aggugcucgu ccacucucag ugcaaucaag caaaagagaa gcagacaccu     1920

cugggcugga uuaacaaugg gggagguuuu ggccagagca aaaacgaaua cacuaaucga     1980

gugaagaaca auaagaaaua uaguaagaac aaagucgaca accuucugaa ugagaccgac     2040

uugacagaua ucaucaccca gagaucauuc auucaaagga accucaauga cacucgcuac     2100

gccgcucgga cggugcugga ugaguuuuau uccuucuuua agucuaacga cuuaccaaca     2160

aaaguuaagg ugguaagagg gaaguggacc ucgcagauga ggcgcaaaug gaauaacguc     2220

ggaggccugc gaaagacucg ggauacccac caucaccaug ccaucgacgc aagcauuaua     2280

gccuccuucc cgcuacuccg ugcuuuugau agagccauca aacugauuga cguggauaag     2340

gaaacaggug agaucguuca guacgggaaa gcaucacuua auggcgacgu gauugaagag     2400

aagaguguca uccaagcgcc cauaaucaaa aacgaaaagu ucgagaaguu gguggaugag     2460

auuuacaauu uuccucuguu caaacaggua gaacucgcua acaauaucga gaacccagaa     2520

aaucccguga aguucucuca ccauguugac aaaaaggcca acaggaccgu cgcaaaucag     2580

acuauuuaua gcacacgcau caaaaagacg gagaagauag ugcggaaaaa ggugaccgcc     2640

aaagaggaag aguacgucau uggaacuauc aaggauaucu augacguuac aaacuuugaa     2700

aaauucaaga agcuguacga cgaguccagc aaaaaggaug acauuaaauu uuuaaugcag     2760

aagaacgauc cuaaaaccug ggaaaagcug guggagauaa ugaauacaua uccagacuac     2820

gaggaaaucu cacaacuuga uggcaaggug aaaagaguaa aggucucucc cuucaaauug     2880

uauuuuaggg agaacggguu cauuacuaag uacgcuaaac acaauaacgg accgaaggug     2940

guuuccauca aguacuauga caguaaacuc gguaaucaca uugauaucac cccugugaac     3000

gccaauaaca aggucauacu gcagagccua aaacccuggc gaacggacgu guacuauaau     3060

aacgaaaccc agcaguacga gcugcucggc cugaaguauu cugaucuuaa auuuacaaag     3120

gggguuuacg gaaucacucg cgaagcauau gagaaauuga aguacggcua caccgacggu     3180

gugccagcuu ggaagccuau uuccaaagag ucagaguuca aguuuagucu guaucggaau     3240

gauagaguaa ggaucauuaa cgaggccggg gagaaaaucg acuuacucuu cggcagccgu     3300

aaugaaacau cggaaggaua cgucgagaug aagcccauau uuaaaacuaa cuucgacgau     3360

gcggaggaag ccuccuuuua uggcaagacc ucuaauaagc gccugauuaa aaagauggcu     3420

cggaaagggu accaaaucua uaaggugaac acggacaucc uuggaaaucu guacuauaca     3480

gcaaaagaga gcaacgaacc aaaguuggau cucuga                               3516


<210>  2866
<211>  3519
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2866
augagaaagc ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

auaacagaug acuauaaagu uccuucuaag aaaaugagga uccaggguac uacggaucgg      120

accucaauua agaaaaaucu caucggcgca cuucuguucg acaacgggga gacagcugaa      180

gccacucgcu ugaagcgaac cacacguaga agguacaccc ggcgcaagua uagaauuaaa      240

gaguuacaaa agaucuuuag uagcgaaaug aaugagcugg auauagcguu cuuuccaagg      300

cuauccgaau cuuuccucgu gucggacgau aaagaguuug agaaccaccc gaucuucgga      360

aaucugaagg acgaaauuac uuaccauaac gauuauccca caaucuacca ccuucggcag      420

acccuggcug acucagauca gaaagccgac uugcgccuca uuuaucuggc acuggcccau      480

aucauaaagu uucgaggcca cuuccucauu gaggguaauu uagauagcga aaacacggac      540

guacaugugc uguuucuuaa uuuggucaac aucuacaaua accuguucga ggaggacauu      600

guggaaacug cuaguaucga ugcagagaag auccuaaccu ccaaaacauc uaagagcaga      660

aggcucgaaa aucugauugc cgagauaccu aaccagaaac ggaauaugcu uuuugggaac      720

cugguuuccu uggcucucgg acugacucca aacuucaaga ccaauuuuga auuacuggag      780

gacgccaaac uccaaaucuc aaaggauagu uacgaggaag accuugauaa ccuguuggcg      840

cagauuggcg accaguaugc agaucuauuc aucgccgcua aaaagcugag cgacgccaua      900

cuccugucug auauuaucac agugaagggc gcauccacca aagcuccccu uucagccagc      960

augguccagc guuacgagga acaccaacag gaccuggcgu ugcucaagaa ucuggugaaa     1020

aagcagaucc cugagaaaua uaaggaaauu uucgauaaca aggagaaaaa uggguacgcc     1080

ggauauaucg acgguaagac gagucaggag gaauuuuaca aauauauuaa gccaauauua     1140

cugaaacuag acggcacuga gaagcucauc ucuaagcugg aacgcgagga uuuccuuaga     1200

aaacaaagga cauuugacaa cggguccauu ccccaccaga uccauuugaa ugaacugaag     1260

gcaaucauuc ggcgacagga gaaauucuac ccuuuucuca aggagaacca aaagaagaua     1320

gaaaagcugu ucaccuuuaa aaucccauac uauguaggac cgcuugcuaa uggccagagc     1380

ucguucgccu ggcugaagcg ccagucuaac gaguccauua cucccuggaa uuuugaagag     1440

guuguggauc aggaggcuuc agccagagca uucaucgaaa ggaugacaaa cuuugacacc     1500

uacuugccug aggaaaaagu ccucccaaag cacagucccc uguaugagau guucauggug     1560

uacaaugaau uaacgaaagu gaaguaucaa accgagggua ugaaacggcc ugucuuucug     1620

agcuccgagg auaaggaaga gauuguuaac cuacucuuca agaaagaacg caaggugaca     1680

guaaaacagc ugaaggagga auacuucucu aaaaugaagu gcuuucauac ugugaccauc     1740

cuuggggucg aggacagauu caaugccagc uugggaacau aucacgaucu gcucaagaua     1800

uuuaaagaca aggcuuuccu ggaugacgag gcgaaccagg auaucuuaga agagauugug     1860

uggacucuga cccuuuuuga agaccaggca augaucgaga ggcguuuggu uaaauacgcc     1920

gauguguucg aaaagucagu ccucaaaaag cugaagaaac ggcacuacac aggcuggggc     1980

cgacuaaguc aaaagcugau uaacgggauc aaagacaagc agaccggaaa aacuauacuc     2040

gguuuucuga aggacgaugg cguggcuaau agaaacuuca ugcagcuuau uaaugacucc     2100

agcuuggauu uugccaagau cauuaaaaac gagcaggaga agacgaucaa aaaugaaucu     2160

cuggaggaaa caauagcaaa cuuagccggg ucaccagcua ucaagaaagg cauucuccaa     2220

aguaucaaga uuguagacga gaucguuaaa auaaugggac agaaucccga uaacauugug     2280

aucgagaugg ccagggaaaa ucagagcacc augcagggua ucaagaacuc ccgccaacgg     2340

cugagaaaac uugaggaagu ccauaaaaau acugggucua agauucugaa agaguauaac     2400

gugucgaaua cccaguugca gagcgacagg cucuaccugu aucuacugca agauggcaag     2460

gacauguaca caggaaaaga acucgauuau gacaaccugu cccaguacga uaucgaccac     2520

auuauaccgc agucauucau caaggauaau aguauugaca acaccguccu uacuacacag     2580

gcaucuaacc gcggcaagag cgacaaugug ccuaacaucg agacgguuaa uaaaaugaag     2640

uccuuuuggu auaaacaauu gaagucaggg gcgauuagcc agcgaaaauu cgaucaucug     2700

accaaggcug agcggggagc cuuaucugac uuugauaagg cagguuucau caaacgucag     2760

cucguggaaa cuagacagau aacaaagcac guagcccaaa uccuggacuc cagguucaac     2820

aguaaucuua ccgaggauag caaaucuaac cgcaauguca agauuaucac acugaaaucc     2880

aagauggugu cagacuuucg gaaggauuuc ggcuuuuaca aacuaagaga aguuaacgac     2940

uaccaucacg cucaggaugc cuaucucaau gcaguggucg ggacugcucu guugaagaaa     3000

uaccccaagc uggaggccga auuuguguau ggagacuaca aacacuauga uuuagcgaag     3060

cucaugauuc agccagacag uagccugggc aaggccacca cgaggauguu uuucuacucg     3120

aaccuuauga auuucuucaa aaaggagauc aaacuggcug acgauaccau auuuacacga     3180

ccucagauug agguaaacac ugaaaccggu gagaucgugu gggacaaggu uaaagauaug     3240

caaacaaucc gcaaggucau guccuauccc caggugaaua uugugaugaa aacugaaguc     3300

cagaccgggg gcuucucuaa ggagagcaua uggccaaagg gagacucaga uaaauugauc     3360

gcacggaaga aaaguuggga ccccaagaaa uacggcgggu uugauucccc uauuaucgcc     3420

uacucuguuc ucgugguggc uaagaacugu aagaggaaaa auagcaagac gaaaaacuau     3480

caaagaacau gcggaaacaa gaauaacggu acccguuga                            3519


<210>  2867
<211>  3522
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2867
augagaccca ucgagccuug gauucugggc cucgacauag ggaccgauag ccuuggaugg       60

gccguguucu ccugcgaaga gaagggucca ccgacagcua aagaacuguu gggcggggga      120

gucagguuau uugacucugg ccgggaugca aaggaccaca cuucacgcca ggccgagcga      180

ggugcguucc guagagcuag gcggcaaacg cgcaccuggc ccuggagaag ggaucggcug      240

aucgcccuau uucaggcagc cgggcucaca ccuccagcug cagaaacucg ccagauugcc      300

cuggcccuuc gaagagaggc uguuaguagg ccccuggcgc cugacgcauu gugggccgcu      360

cuccugcauc uggcccacca ucggggauuc cguagcaacc gcaucgauaa aagagagagg      420

gcagcugcca aggcgcucgc caaagcaaag ccagcuaagg ccaccgcuaa agccacagca      480

cccgccaagg aagcugacga ugaggcgggc uuuugggaag gcgcagaggc cgcuuuacgg      540

cagcgaaugg ccgcauccgg ggccccuacc gugggagcuc ugcuugccga cgauuuggac      600

cgcggucaac caguaagaau gagguacaau cagucugauc gggacggcgu ggucgcaccg      660

acucgcgcgc ugauugcuga ggaacuagcc gagaucgugg caagacaguc gucagccuau      720

cccgggcucg acuggccugc uguuacaagg cuggugcuug aucagcgucc acugcggagc      780

aaaggagccg gccccugugc auucuugccu ggugaagacc gagcucucag agcccugcca      840

accguccaag auuuuauaau caggcagacg uuagcgaacc ugcgccuccc caguacuucc      900

gccgacgagc cgcggccucu uaccgaugag gaacacgcua aggcacuggc cuugcuaucu      960

acagcuagau ucguggagug gcccgcccug aggcgcgcac ucgggcugaa acgaggagua     1020

aaguuuacug cggaaaccga gcggaauggc gccaagcagg cugcccgugg cacagcaggg     1080

aaccuuaccg aagcuauucu ggccccauug aucccuggau ggagcgguug ggaccucgau     1140

agaaaagaca ggguuuucuc cgaucugugg gcagcccgcc aggaccgguc agcuuuacug     1200

gcgcuaauug gcgaucccag agggccaacg agggugacug aggacgaaac agccgaggca     1260

gucgcugacg ccauccaaau agugcucccc accggacgag ccagucugag cgcuaaggca     1320

gcccgcgcga uugcucaggc cauggcaccu ggcaucgguu acgaugaggc cgugacucuu     1380

gcuuuggggc ugcaucacuc ucaccggcca agacaggaaa ggcucgcacg ucugccguau     1440

uacgccgcgg cccuucccga cgucggccug gauggagacc cuguuggccc acccccugcu     1500

gaggaugacg gggcagccgc ugaagccuau uacggacgca uugguaauau cuccgugcau     1560

aucgcauuga acgagacacg gaaaauugua aaugcucucc ugcacagaca uggcccaaua     1620

uuaaggcugg ugauggucga aaccacgcga gagcuaaagg ccggggcgga ugagcggaaa     1680

cgcaugaucg ccgaacaagc agagagagaa agggagaacg cugagauuga cguggaacuc     1740

cggaagucag aucgauggau ggccaaugcu agagagcgca ggcagcgugu ucggcuggca     1800

agaaggcaga acaaucuuug ccccuauacc agcacaccga ucggacacgc cgacuugcug     1860

ggcgaugccu acgacauaga ucacgugauu ccucucgcuc gcggugggcg agacagucug     1920

gacaacaugg ucuuauguca gucugaugcg aauaaaacua agggcgacaa gaccccaugg     1980

gaagcauuuc augauaaacc cggauggauc gcccaaagag acgauuuccu ggcucggcuu     2040

gacccucaga cagccaaggc auuggccugg agguuugcug augacgcggg cgagcgcgug     2100

gcccguaaau ccgcagaaga ugaggaccag ggguuccucc ccagacagcu gacugauacc     2160

ggauacaucg cucggguagc ccuaagguau cugagccucg uuacaaacga gccaaaugca     2220

guggucgcca ccaacggucg ccugacuggc cuuuugcgac uggcuuggga cauuacgccu     2280

gggcccgccc caagagauuu acucccgaca ccucgggacg cgcugaggga cgauaccgca     2340

gcccgcagau uccuugacgg acugacuccc ccacccuugg cuaaggccgu ggaaggcgcu     2400

guccaagcaa ggcucgccgc ucugggucgg ucgcgugugg ccgaugcagg gcuagcggac     2460

gcccugggcc ucacccuggc uucucuugga ggcgggggaa agaaccgcgc cgaucaccga     2520

caucacuuua ucgacgcagc uaugauugcc guuacaacca gaggcuugau aaaucagauc     2580

aaccaggcau ccggugccgg gaggauucug gauuuacgga aguggccucg cacuaauuuc     2640

gagccacccu acccuacauu uagagcugaa gugaugaagc agugggacca uauccacccg     2700

ucaaucaggc cagcgcaccg ggauggaggc agucuccaug ccgcaacggu auucgguguc     2760

cgaaacagac ccgacgcucg cgugcugguu caaaggaaac cuguggagaa acuuuuucug     2820

gaugccaaug ccaaaccacu acccgcagac aagauugcug aaauaaucga cggcuucgcc     2880

agcccucgga uggcgaaacg uuuuaaggcu cuccuggcca gauaucaggc agcccacccg     2940

gaggugcccc cagcuuuggc agcccuggcg gucgcuaggg auccugccuu cgggccccgc     3000

ggaaugaccg caaacacugu aauugccggc cgguccgacg gggauggaga ggacgcuggc     3060

uuaaucacac cauuucgagc caauccuaag gcagcuguga gaaccauggg uaacgccguu     3120

uacgaagucu gggagauuca ggugaaaggg aggccccgcu ggacacaucg ggugcucacu     3180

agauucgauc guacccagcc agcgccgccu cccccacccg aaaaugccag gcuggucaug     3240

cggcuucgcc gaggagaccu gguuuauugg ccuuuggagu cuggcgauag acucuuucug     3300

gugaagaaaa uggcagugga cgguaggcua gcucuguggc cagcccggcu ugcaacgggc     3360

aaggcuaccg cccucuacgc ccaacugagc ugccccaaca ucaauuugaa cggggaucag     3420

ggauauugug uacagucagc ggaaggcaua cgcaaagaga agaucagaac aaccaguugc     3480

acugcucugg ggagguuacg acucuccaag aaagcaacau ga                        3522


<210>  2868
<211>  3525
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2868
augggcaagu acagcaucgg gcuggacauu ggaauauccu cuguggguug gucaaucauu       60

gauaucgaga aaaagaaaau ugucgaccuc ggcaguagaa ucuuccccag cgggaacgcc      120

gcugcaaauc aggauaggcg gggauaucgc ggcacccgac gucuuauaag aaggcggaag      180

aaccgccugg ccgacguuau caacuuuuug ggugaagaga auuucauuga uacaaaagug      240

guagaccaaa augggaacau ccacuacaag uuacugguaa auuccacuga aucuccuuau      300

aagcuaagag ucaaaggacu cucgaagaug cugacgaaac aggagcuugu gauugcgcug      360

uaccauaucg uuaagcacag gggcauauca uaugauuugg gcgaccucga agaugacggg      420

accagcggag ugacagauua caaaaccagu auuaacauca aucggcagcu gcugauuaag      480

aagacugucg gucagaucca acucgagcgc uuaaaagagu uuggcaaggu gcgagggcau      540

gugaaaaagg acgaaaacau cacacugcuu aauguuuucc cauccucugc uuaugugaaa      600

gaggccaccc agauuuugac uaagcagaga gaauuuuacc cggagauaac agaugacuuc      660

aucaccaaau uuauucccuu caucagcagg aagcgggacu acuuugucgg accuggcaac      720

gagaaguccc guacggauua ugguauauac aaaaaggacg ggcgcacucu ggauaaucua      780

uucgacgaac ucauuggaau cgauaaaauc ucaggcgaga agagagcaag ugccagcucu      840

gugaccgcuc agauuuauaa caugcugaau gaccuuaaca accugacagu gacuaauacc      900

gaagauggca aauugacaac ccaaacgaag agggagauca uugaagcaga caagacuaca      960

aacgggcucu uuggaauaac ccagaucugc aaaauuaucg guuguaagaa agaugacauc     1020

aagggcuucc ggauugauaa agucgagaag ccaauaaugc acacucuguc cggguaccga     1080

cgcuuuagaa aggaguuagc caaaaucaau auugaagcua acgaccugga caucaaucuc     1140

guuaacaaga uugccgauau ccuuacacug aauaccgaga aagcggaaau aaggaagcag     1200

uugaacaaau caaauaucga cgugagcaac gagauuaagg aacuaaucau uaauaagaac     1260

aaagauuucc ugguagacgg acgggcaacg uggcauaguu ucucuuauaa gacccucaau     1320

cugcuuaucc ccgagcugau gaacacaucc gaggaacaga uaacuauuuu gacccgccuc     1380

ggccugguga aaagcaauuc ggagaaguac aaagguuuaa agaacauccc ugccagaagg     1440

auuacagaag ggaucuauaa cccagucgug gcuaaagcca cucgugaggc aguuaaugug     1500

uuuaacgcua uagccaagaa gaucggccgg gauaauauuu cugacgucgu gaucgaacug     1560

ccccgagagg auaacgaggc ggacauuaaa aagggaaucg uaaaaagaca aaagcagaau     1620

gaacuagaga aagaagccgc agaugcucuc guuaagcagc aaaugaccau auccgacuca     1680

gcccuggugg cucacuacag gaagguccgc ggccuuaguc agaaagugcg guacugguau     1740

cagcagggga caugcugucc uuacugcgga aagaacauug aggccgucca auugaucaau     1800

aacaaugaua gcuucgaggu ggaccacauc auuccaaucu ccguuucuuu ugaugacagc     1860

cagaacaaua aagugcuggu acauucacag uguaaccagg caaaggguaa acaaaccccg     1920

cucggcuggc ugaauaacgg gggaggcuuc ggucagaaua agacugaaua ugucgccaag     1980

gugaaaacga acaagaacua caguaaaaau aagauggaca accuucugaa ugauauggac     2040

uuguccgaua uuguuacaca gagaggguuu auacagagga accucaauga cacccgcuau     2100

gcuagccgaa ucgugcugga ugaguucuac ucauuuuuca aaucuaacaa uuuaaguacu     2160

aaggucaaga uugugcgggg caaauggacc agccaaaugc guaagaaaug gaacggagua     2220

ggcgggcuga agaaaacaag agacaccuau caccaucacg cgaucgaugc auccauuauc     2280

gccucuuuuc cccuacucaa gguguucgac aaagcuauaa agcugaucga uauugacagg     2340

gaaacuggag agauccuuca ggauaaggaa gccauuaaaa ugucgcagga ggcaaaguug     2400

cuggagaaaa ucccuguuau acagaaugaa aaguuugagg aacucauuaa cgagcuguac     2460

gacuucccau uauuuaaaca agucgaacug gccaaugaua ucacaaaccc cgagaauccu     2520

gugaaguuca gccaccaugu ggacaagaaa gcuaaccgcu ccgucgccaa ucagacgauc     2580

uacgguaccc ggauuaagga gaaagaaaua aacaagagag gcaaaguuga gaaggugcag     2640

gaaagguaua uccuugggac uauuaagaac aucuacgaca uugauucauu cacaaguuuu     2700

aaaaagaucu augacaaaau gucuaagacc ggagauguga aauucuugau gcaaaagaag     2760

gacccaaaaa cauggggcaa gcucguagag auacugaaua gcuaccccga uuccgaagag     2820

aucacucagg ucgacgguaa agugaagcga guuaaagugu caccguuuga gcuauaucgc     2880

cgggaaaacg auuucauuac caaguacgca aagcacaaug acgggccuaa aaucgucagc     2940

auuaaguauu acgauucuaa aaucggcucc cauauagaca uuacgcccau caacgcgaag     3000

aauaaagugg uucugcagag ucucaagcca uggagaaccg auguguacuu uaacagcgag     3060

acaaggcagu augaacugcu uggauugaaa uacucugacc ugaaguucac uaauggcgua     3120

uaugggauca ccuccgaggc uuacgagaag uuaaaacacg gaauuucaga agacggcaca     3180

aagacuugga aaccuaucgg uaagaacagu ggguuucguu ucagccucua ucgcaauaac     3240

cgggucaaaa uaguggauga gaagggagaa ucgcuggagc uucuguuugc cuccagaacc     3300

acgucuaaug aaggcuacgu ggaguugaag cccauuuaua aaagcaaguu cgacucaaaa     3360

gaggaugucg guuuuuacgg ccaaaugaca ccaagugggc aguucguuaa gaaaauggca     3420

agggugggau acaagcucua uaagguaaac accgacgugc ugggcaaucc cuacuauauc     3480

acuaaagaau ccgaugaccc ugagcuaucu cuguacaaga aauga                     3525


<210>  2869
<211>  3528
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2869
auggaggaaa ugaagcagac cggcagaaag aacgaggccc ccaagcugac agaccucaau       60

uacaggaucg ggcuugauau uggaauagcu agcguggguu gggcaguccu ggaaaacaau      120

ucccacgacg agccuuuccg gaucauugau uugggcguuc gcaucuuuga cacugccgaa      180

caaccaaaaa cgggggcguc uuuagcucug ccgcgacgug aggccagauc agcaaggcgg      240

cgcauuagaa ggcggcgcga ucgaaucgag agaauaaaga aacuauucca ggccgaagga      300

cucaucaaca uugacgaguu ugaagcuagg uauaagaucg caggccugag ugauguguac      360

aaacuucggu augagggucu ggaccguaaa uugaccgaug acgagcucgc ccagguacug      420

cugcauauug cuaagcaccg cggguucaaa agcacaucca aggccgaaga ggcggaaaau      480

gagaacgaug cagccaaaaa gaaggaagcu ggagcccuca aaaagggcau cucuaauaac      540

aaaaaguuaa uggaggagaa aggcuacaga acuguggggg aaaugaugua ucuggacgag      600

aauuuuagga ccgauugccc cuggaacgaa aauggauaca uacuuacacc ucggaacaag      660

ucgggugacu auaaacauac cgucuugcga gacaugcugg uggaugaggu ucgcgaaauu      720

uucaagucac agagaagccu aggcaauauc cacgcaagug aggagcucga aaacgcuuac      780

cuggacauua ugcuuuccca aaggucuuuu gauauggggc caggagcccc cagcaaguac      840

gcgauggagg gcuucgaaga ccgggugggu cuguguacuu uugaggggaa aaauggagaa      900

aagcgcgccg caaaagcugc cuauacaucc gaguuguucg ucgcucucga gaagaucaac      960

cauaccaaaa ucgugucaga ugacggcgcc acgagauuuc ugacuaccua cgaaaggaag     1020

aguauuauag aguuaaugca caagcagcag acaguaaaau augcagccgu ucguaaggcu     1080

aucggccugg cggaagagga uaaauucuac aaccucaauu auagcccuaa ggcagggucu     1140

aaaaaguccc cagaggacac ugauuuugug aaacuugaaa acuaccacaa aauucggaag     1200

gcccugggag aggacaucgu cucagaacau uugcccgcug agaaaauaaa gcuauaugau     1260

agcauugcca ccauccugac acucuucaaa aaugacgaca gucgaaucaa gagauucauu     1320

gaacugggua ucggcgagga gugcuuugau gggcuucugu cuuugucccc uagcaaguuc     1380

cacaaccucu cgcugguggc aaugggaaaa auuauaccau uuuuacugga cggcaauacc     1440

uacgauaagg ccugugaacu agcugguuac gacuucaaag ccgauaacaa gggggagaaa     1500

ucugugcucc ugaagggcaa aaauaucacg gacaugguca acgauauuac uaauccgguu     1560

gugaggcgcu ccguaucaca gacaaucaag gugaucaacg caauuauacu ugaauaugga     1620

aguccccaag cggucaauau cgaguuggcu cgggaaauga gcaagaacuu ugacgagaga     1680

aggaaacuga aguccgauau ggagucucgc gaaaaagaga auaacaaugu gauuaagcga     1740

guuaaagaau acaagcucaa cccuaccggc caggacaucg ugaaguuccg gcugugggag     1800

gaacaggauc guagaugccu uuauagcggg gagaaaauug acaucaacga ucuguuuuca     1860

agggacggag guuacgacgu cgaucauaua uugccauaua guaucacuuu cgacgauaca     1920

uaccgcaaua aggugcucgu aaaagccucc gagaaccggc agaagggcaa uagaaccccc     1980

uaugaauacu uuauggcaga gaaagacgau gacgaaugga acguuuacga gacgcuggug     2040

aggaauaccg ucaaggauua uaagaaagug uuaaagcugc ucaaaaagca cauuacagag     2100

gacgaaaaga gcgaguuccg agaacgcaac cuaacugaua ccaaguacau cacacggauu     2160

aucuauaaua ugauaagaca aaaccugguc cuuaagccuu acuugucucu ggagaaagaa     2220

aggaagaaac aggugauggc cguuaauggg ucaauuacuc acuaucucag uaagcguugg     2280

ggacuggaga gcaaagaccg ccggaccgau acacaucacg cucaggacgc cgugguaauc     2340

gcauguacca cugauggcau gauuaacaag aucuccagau acucuaaagg uagggaguua     2400

cuguauucgc gaggguuuaa guucgucgac gaagagacgg gcgaaauacu uagccgggac     2460

aauuuuacac gcgaggaaug ggaugagaag uacggaguga aaaucccaau ucccugggag     2520

cauuucagag acgaauugga uaucaggaug ucccgguaca acggcaccgg ggacgauuca     2580

auugacaaga cuaccccgcg agauuucauc cucagucaca gagacgcuca uaaauggaua     2640

aauuauccug aguggauguu uguugaagga cgccccggua ggcguggcgu gcuggauggg     2700

auuuucgucu cucggaugcc aaagcacaaa gugagcggac aagcccacga ggaaacagua     2760

agauccgcga aguacuauaa cgacggcggu ucaguguuug agagcggggg cuacguuguc     2820

accaagaaau cuauccagga uaucaagcua aaagacggag agauuaagga guucuuuaau     2880

ccugacuccg auaggcugcu cuauaacgcc cugguggacc gccuuaacaa auucgaaaau     2940

gaucccaaga aggcuuuugc agaaccauuc uacaaaccca aggccgacgg cacucagggg     3000

ccuaucguga aaaagauuaa agucuuugag aagcagagua gcggaguuuu cgugcgagau     3060

ggcaagggua uagcugccaa cggggcaaug gugagaaucg acguauuuug cgaggaagau     3120

aaaggaaaga aaaaguauua cuucguccca auuuacacag cggacguggu uaaaaagaag     3180

uugccggauc gggccgcuac ggccaauaaa cugaucucug aguggagggu gaugaaggaa     3240

gagaacuuua uuuucuccuu auauucacgc gaccucaucu acauagaaag ugauaaaggc     3300

guccaaguga aggacaucga ugguaaaauu agcaagcccg uuaaucuguu cgcauauuac     3360

cuuggcgcug acaccucgac ugccuccauc ucugggaaac ugcaugacag cucagcaaac     3420

uuucguagua uuggaaucca gggcuugaga cgguucgaua agugucaggu ggacguacuc     3480

gggaauauau ccuuugucaa caaggagaaa aggaugccuu ucaauuga                  3528


<210>  2870
<211>  3531
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2870
augaacaagg ugcaggagga cugcuacaaa aagaucaaag aaaagcuggc cgauagagag       60

uauaggauug gccucgaccu uggggucgga agcauagguu acgcuguugu gucccugaaa      120

ggcgauaugc ccgaagacgu aaucuuggca gggucucgga uuuucaaguc aaguaucgga      180

gccgcggagc gccgacacuu ucgugcucaa agaaauaccc auaggcacca ucgggaacgc      240

augagauauu uauggaagcu gcuagccgag aaaaaccucg cacugcagcu uccugagaau      300

cugagcaaga aagaaaacuu cacagauuuu gacacuucca ggaagcgguu cccaauugag      360

guguugcgca augauccgua cauccuccga uauaaagccc uggacgaaaa gcugucucuc      420

uuucaguuag gcuacgcucu guaucacaua gcaaaccaua gagguacggc cucggucagg      480

ucauuccuug aggaugacga ggcuaaggug aaagaaaaua aggagaccgc caaauuggcg      540

aagaacacaa agaagaucau guacgagaaa aaguaccgga cuuuugggga auaucuguac      600

cgugaacgca uugagaaguu cagacagaau gagaggauga aaguuaccaa caagaaugaa      660

aacaaagagu uugcagugac acgggauauc auugaaaagg aggucgaaau gauccuagga      720

gcccaaaaac gauauaauac cgcugugcuc gccgacgauu acauagagcg cauuaagaag      780

gcaaucuucu augagacuga aaaacugauu cccgagccug aauacugucc auauuuuaag      840

gacgacaaaa gacuucccag gagccacaag cugaacgagu accggcgcau cuacgaggcu      900

uugaauaaca caagauauua caucccugaa auugaggaga aaaccggcga aauacucagu      960

aggaaggaaa aggagcagcu guccaaagag gaaaaggaga aauuauucaa cuuucugcuc     1020

aagaauaaag aacuuacgga ggccaaggua aagaaacugu ugaaccuauc ugaaauggcg     1080

aagguucagc ugcagggccg ugauaaaaag aaucagaaaa ucaaggggua ugcccucauu     1140

acucuggaga gccuuccauu cugguccaaa cugcccgagc aacagcagga cgaguucuug     1200

uacgauugga acaagcuccc ugacgaaaag cugaaagauc gguuacgaaa uuauuacaag     1260

cugaccgacg augaaaucga cgagacauuu caaaacauag ugcuaccauc aaauuaugca     1320

ccgcucggua aaaaggcuau ggaaauucug cuugaguaca ucaaaaacgg auugaguuac     1380

aucgaggccc uggauaaggc ugccgacgaa aauaagauua aagucgagaa gcacgaaauc     1440

aacgaucucc ugcccuauua cggcaaaauu cuucaggaga gcacucagau gccuauaggg     1500

aaggcauucu cuaaacaauu ugccgcuaag aauuauaaga aaccagacau caacaagaau     1560

gaacaggagu ucggaagaau ugcgaacccc guggugcauc agacccugaa ugaguugagg     1620

aaacucauca acgaaaucau uacagucuuu ggcaagaaac cucuggagau auccuuagaa     1680

accucacgcg agcugaaaaa gagccagaaa gagcgggaac uaaucgcaaa gcaacaggac     1740

auugaugagg agaaaagaaa gaaaaucugg aagacguaca uugaaccaca ccuccagcag     1800

aucaaaagua ggucugacaa gcugaagaaa cuuuucguuu uugaauugug ggaggaucaa     1860

aagcauaacu gccccuucug ucuggagaaa auaaaugugg acgauaucua uucccgcggu     1920

guagacaagg aacaccucuu uccgcugaaa aagagcgagg augacucgga aaacaauuua     1980

gugauugccc acaacaagug caaugcucga aaagccgagc gggcaccuuu cgaugccuuu     2040

gggcaugaau cuaucuggaa cgacauuuuc uccaaugcug agaagaaccu gccccguaaa     2100

cuuuggagau uuaggcaggg aucauucgag aaguucuugg aaaaaccacc uaugcucaag     2160

cgcuuugaga cugauaauag uuacaucagc aagauauccg cccgguaucu ggcaugucua     2220

uucgacaaac ccucuaagau cuuuugcguc aaaccauucc ugacagcgca gcucagaauu     2280

gcuuggcagc uggaugaaau uaugaucagg cuugccgaga acauaaucuu ggacagcgaa     2340

aaggacucau uuaauaaagg cggccugcaa aagaagaaac gaauugauaa ccgccaccau     2400

gcauuagacg ccgugguuau cgcuuacgcc agucgggggu accacaauuu ccucaacaga     2460

gugcaugcaa agggauauaa aauuaauuac gaugagaaga ccuggcuguc cgagacucuu     2520

aggcccccug guaacaaaaa ccuggaagac uuuaagagcg gcaucauaaa ugucaugauu     2580

aacgcuaaua ucucuaucaa gcacgaucac aacaauaaag ggcaguuggu gaagucaaaa     2640

gccuauaagc ucuacuauag ugagauugga aaauacauca uuacaacccu uaagagcgua     2700

uccgaaauac uauucaaaca gaaggagaag ccacaggaca cgcugcgucg cgcgcucugu     2760

aaauuugagg aucggcuguc ugacaucaag gauaaaaagc ugaggaaaaa guuggaagac     2820

aacauuacca ucuauaaguc gauugagaau gaaagcgccc uguccaaagc uaagcaagag     2880

uuagaagagu ucgaucucaa agcaaagaaa gagggcaaga aggguuacga gaaaaucaca     2940

gacauacuga ucuacaagaa agccugcuca augcaugggg gcaaguauau ucacguuaaa     3000

aacagaaucg aaaauaaguu uuucgcucuu aaggaaccga cugagauuga aaccggauuu     3060

ggcuacgaua cagccgacaa ccugagucua gaccucuauc augauaauaa cgggaaacug     3120

uguggagagg ugauccgaaa gauagacuuc aaucggaacc gcguccccaa uuacaaaaag     3180

gaggguuaua uuuugcugga aagaaucuac cagggcgaua cuuuagaggu ggacaucucu     3240

gaugacaaaa uuagccucaa gaacaaaguc ggguccgcac cugauaagag gaccuucgug     3300

aagguucuga cauuuaccga aucagaccgg uaucuuaaaa acaagcgaga ucagauacaa     3360

aucuucuuuu gcaaucuguu gaaaagcacu cucaagcacg acgauucuuc caaacacgac     3420

gacaguuucu acgugagcuc uaugcagaag uacaacguaa gaaaggucau ucugacgucc     3480

cuaggaauca uuaaauaucg cucaaagauc cugaaaaaua aggagauaug a              3531


<210>  2871
<211>  3531
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2871
augcuguucc ccaagaaacu cagcaaggag gaaguggccu acguccuugu ugagaucaac       60

uccaaucuga acaauucuuc aaacuauuug ggcaauauua gugacagaag caaagaauua      120

uacuuuaaca agcagaccau agggcaauuc cuguauacaa aacuaaagga ggauaggcac      180

uacucccuca agaauaaagu guuuuaucgg caggacuacc uggaugaauu ugagcgcauc      240

ugggagacuc aggcuauuuu ucauaaggaa cuuacggagg accugaaaaa gcagauccga      300

gauaccauua ucuucuauca acguagauug aaaucucaga agggacucau aucgcugugc      360

gaauuugaga acaaggagau cacaauugaa aaagagggua agacuguaac caaacagauc      420

ggcgugaggg ucauuccuaa gucaagccca cuguuccagg aauuuaaaau cuggcaaaua      480

cucaauaacg ugauuuguaa gaaaaagggg acacggaaga aaaaggcagu uaccccggcc      540

cccuuacugu ucgagcuuuu uggaacuccu agugaaacau ucuuuuccuu ggacauggag      600

ugcaaacagc ugcuauucga ggaacucaau cugaagggca accuuaaagc ggauaaggug      660

auccgccugu uggguuacau uccacaggag ugggaaauca auuacaacca gcucgagggg      720

aauagaaccc aaaagucucu guaugaagca uacuuaaaaa ucauugagau agagggauau      780

gacaucaaga cguuccugag gauuaaaagc gauaaggaag acugggaucu cgaggaauca      840

ccccuuuccg ccaaagagau ccgggaaaac auacagcaca uuuuugagac ucugggcauc      900

aguaccgcaa ucuuguacuu cgacgccaca cuaccuauua aggauuuuga gaagcaggcu      960

agcuaucaac uguggcaucu ccuguacucu uaugaauccg acaacucacc aagcggcaau     1020

gagacucuuu acaaacuguu gcaggacaag uucggguuua aacgcgaaca cgcagucuuc     1080

cucaguaaca ucucuuuuca ggaugacuac ggaucccuga gcgccaaggc uauucgaaaa     1140

auauaucccu acaucaagga gaaucaguau ucgaccgccu gugaguuagc ggguuacaga     1200

cauucuaagc ugucccuaac aaaagaagaa cucaacaaua ggccucugaa ggaucuuauu     1260

ucaccauuga aaaagaacag ucugcggaau ccggugguag aaaaaggccu caaccaaaug     1320

aucaauguug ugaacgcacu gaucgagaag aauagcgaaa aagacgccga ggggaacauu     1380

auaaaguauu uccacuuuga ugagauccgu auugaacuug cucgcgagcu gaagaaaaau     1440

gcccaggaaa gagcagagau gaccuugaac aucaagaaug cuacgauuga acaggagaaa     1500

aucaagucca uacucaucuc ugacuucgga auuaaaaacc ccacuaagaa cgauaucaca     1560

agguaccggc uguaucagga guuagcccac aaugacuaca aggaucugua caccaacacu     1620

gacauuccua aagaacuacu cuuuagcaag aauaucgaua uagagcauau ugucccaaaa     1680

gcgcgagugu ucgacgacuc auuuaguaac aagacacugg ccuaucgcga uuucaaucuu     1740

aaaaagggcg aaaacaccgc auucgacuuu aucgauucca gcuuggguga ggaggagcug     1800

aaucaauaca uugcuagagu ggagaacgcc uucaagacga aaaauaucuc ugcuaccaag     1860

uauaggaacc uccugaaaaa ggccacagaa auaaaugacg gguuuaucaa ccgggauuua     1920

cgcgagacuc aguacauugc uaaaaaggcc aaggaccugc uuuuccagau caccagauca     1980

gucguuagua caagcggauc cauuacugau agguugcgug aagacugggg ccucaucgau     2040

auaaugaaag agcugaauau gcccaaguau cgggaccuag gccugaccga gauuaucgaa     2100

cgaagagaug ggaggaaagu ggagguaauc aaggacugga caaaacgcaa cgaucaccgg     2160

caucacgcua uggacgcgcu caccguggca uuuacuaagc cuucuuacau ucaguaucug     2220

aaucaccuua acgccagaaa agacgaaaac aauaaguugu acgcugauau ccugggaauu     2280

gagaggaagg aaacgacaaa aauacaugac gagaacggua augagaagcg cgucuucauc     2340

gaaccaauuc ccggcuuucg acaaaucgcc aaaaaguacu uagaggaaau ucucaucucg     2400

cacaaaacca agaacaaggu gguuacuaaa agcauaaaua agaucgcagg gaccaacaaa     2460

aagcaggaga cacugacccc gcggggacag cuucauaaag aaacuauuua uggcaaguac     2520

cacuauuacu ccaauaagga ggagaaaauc gguacaaagu ucgauaaaga aacgauuuca     2580

aaggugagua acccuaucua ucguuacgcc cugcagaaaa gauuggaggc uaaugaaaac     2640

aaucccaaga aggccuuuac cgggaaaaac gcacucucua agaauccaau auaucuggac     2700

gaugcgaaaa cuaagauucu accugagaaa gucaaccuga ucuaccucga agaggacuuc     2760

agcaucagga agaagauugu guccgagaau cccacaucaa aagaauuugu accaaacagc     2820

auaucuaucg agaagauugu ugaugaaggc aucaaacgca uucugaagga gcggcuugag     2880

accuacaaca augaccccaa aaaggcuuuc gccgauuugg acaaaaaccc uaucuggcug     2940

aauaaggaaa agggaauauc caucaaaaga gugacaauua guggcgucaa gaacacucaa     3000

gcauuacaua gcaaaaagga ucaccucggg aaaccaaucc uggacaagga gggacaggaa     3060

auuccggugg auuuugucuc uaccgguaau aaccaccaug uggccaucua uagggaccag     3120

aagggcaauc uucaagagca gauaguuucc uucuucgaag cugugguacg agcccagcag     3180

gggcugccca uuaucgacaa aacguacaag caagaggagg gauggcaguu ucuauucacc     3240

augaaacaga acgaaauguu ugucuucccu aaugagauca caggcuuuaa cccaucagaa     3300

auugaucucc uggaccccaa gaauaaaaag uugaucaguc cuaaccuguu ccgcgugcag     3360

aagauaagca uuaaagauua uuucuuccgg caccauuuag agacuaaugu ugaaaccaac     3420

aauauccuca aggacauuac auggaaaaga ggugggcugu cgcuucugaa gggcaucaua     3480

aaagugagga uuaaccacuu gggaaauauc gucuccaucg gcgaguacug a              3531


<210>  2872
<211>  3534
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2872
augaacaagg ugcaggagau cgaauacaaa aagauuaaag agaagcuggc cgacagagaa       60

uauaggauag gccucgaucu uggggucgga agcaucgguu acgcuguugu gucccugaaa      120

aaguaugacg auuugucuua cuuacccgag gacauuaucc uggcaggcuc acggauuuuc      180

gccaguagca ucggggcggu agaacgccga gaguuucguc uacaaagaaa uucccacagg      240

caucggcgcg agagaaugag guuccucugg aagcugcuug cugaaaaaca gcuggccuug      300

cagccuucua aggaucucga gaaaaaggaa aacucggcag acugcgagac cucacagaaa      360

cgguuuccaa uaaauauccu gaagaacgau ccguauauuc ugcgcuacaa ggcccucgac      420

gagaaauuaa gccuguucca acuuggauau guguuguacc acgucgcuaa ucaucgaggc      480

acagauagua ucagauccuu ucuggaagac acugaugaca agauuaaaga gaacacgaag      540

accgcaaaac uagccacaga aacuaagaaa aucauagauu cuaagaagua caggaccuuc      600

ggugaguauc ucuacaaaga acggauucag cagaaugagc guaagaaagu gagcaaccgc      660

aagacagcug ggaauaaaaa caaggaguuu gccguuacca gagacaugau cuucaaggaa      720

acugagaaaa uucuggcguc ccagaagcaa uauuacacaa augugcuuac cgacgaauau      780

aucaaagaga ucgaaggagc aauuuucuac cagacggaga agcugauacc cgagucaggc      840

uauuguccuu acuucaaaga ugaaaagagg uugccaugca gucacaagcu caacgaguac      900

cggcgaaucu augaagcccu gaauaacgcu cgcuacagcg cccccgucuu agacgagaaa      960

acuggcgaaa uucugucuua ucaggagggg gaggaauuuu ccucagagga aaagaaaauc     1020

cucuucgaug aggugcuucu gaagggaaag aaguugaccg agagcgaaau aaagaaacua     1080

cugcaucucc cugacgguug ugagauucag cugcaaggca gagauaagag gacacagaaa     1140

aucaaggggu acgcacuuac ugcucuggaa gccuugccau uuuggaguaa acucucucag     1200

caacagcagg acgaguuccu guaugauugg aauggauuac ccgaagagaa gcugaaaccu     1260

aagcuaaucg acugcuacuc ccucaccgag aacgaaguag augagacauu ccagaagauu     1320

cggcugccaa gcaacuaugc gccgguuggc aaaaaggcca uggaaauccu uuugaccuac     1380

auuaaagacg guuacucgua uacggaggca cuggauaagg cugugucuga agggaaauuu     1440

aaggucgaca agcaaacuac aauauacgac ucccuccccu auuacggaaa aauccugcag     1500

gagucaaccc agaaacuuau uggcaaagcc uucaguaagc aguuugcuaa acgcaauuau     1560

aagaagccua acacuaauag aaacgagagg gaauuuggca aaaucgccaa uccaguggug     1620

caccaaagcc ugaacgaguu gcguaagcuc aucaaugaaa uuauaacagu cuuugggaaa     1680

aagccccugg agaucggauu agaaaccgca cgggagcuga aaaaguccuc uaaggaucga     1740

gagagccuag uuaaacagca gaacgaaaau gaguacaaca gaaauaagau uuauaaagac     1800

uacguggaac cucaccucuc acagaucaag aguaggcaag agaacccauc ccgcuacauu     1860

uucaaauuug agcuguucga ggaacaaaag agcaucuguc ccuuuugccu uaaagaaaua     1920

acgccggaug acaucauuaa uucuaaggua gauaucgagc auauuuuccc uaucgaagag     1980

ucagaggacg augccaagaa caauuuggug auagcucaca acgaauguaa cgcggacaaa     2040

gguaagaaaa gucccuuuga ugcauucggc gacaagacca gcgggaaaua ugucuggaau     2100

gauauucugc auaacgccaa gaagaaucuc ccacacaaaa ucuggcgguu uuaccaggga     2160

ucccuggaga aguucuuaga aaacaaaccu augcugaaga gauucggcac agacaauucu     2220

uauauuucga aaaucgcugc caaguaccuu gcaugcuugu uuaacaauag cuccaagaua     2280

uucuguauca aaucaagucu cacugcccag cugaggauug cuugggagcu aaacucuauc     2340

augauucccc uggccaagaa uauccucagc gaaaaagagc uggaggaauu uaagacccuu     2400

aaaaacaaga agauacgcau ggauaaucga caccaugcau uggacgcgau uacaaucgcu     2460

uaugccaacc gggguuacca caauuuccug aacaaaaucc augcaaaggg guauaaaauu     2520

gacuaccgug agaagaauug guuauccaaa auccuccugc caccccuuaa cagagaucug     2580

gaagacuuua agauuucagu gaagaacagc auagagaaag ccaauguuuc uaucaagcac     2640

gaucauaaaa cuaacggcuc cuuggugaag ggaaccgcuu acaaagucua uuacaguggc     2700

acagggaagu auauuaucac cacucucaaa aauguggaag agauuagcuu caaggagaag     2760

gaagacccug aggauacgcu gaggcgcgcc cuaugcggau uugaagacuc uaacaucaaa     2820

gaugagaagc ugauuaaaaa gcucaaauac aauuccgacc uguauaagaa gauccuuaac     2880

acaaaaccaa aggcaaaaca ggaguuggaa caggcuaauc aggagaccaa ggaagagggu     2940

aaaaagacuc aaaagauuac cgauguacug aucuacaaaa aggccuguuc auuaguuggc     3000

gaacaguaua uucagugcgc gagucggcuc gagaacaaau ucuuugccgu gaaggagccg     3060

acagaaauca gaaccgggau aggauacgac acuggcgaua aucuguuccu ugaccuguac     3120

uaugauaaaa caaggaagcu augcggugag auuauccgaa agaucgacuu uaaccagaau     3180

aaaauuccca acuaccaaaa ggaaggguau auacucuucg agcgcaucua ccagggcgac     3240

acgcuggaag cugauaccag cgaggacaaa auuucguuga agaauaaaac uggauccgca     3300

cuggauaacc ggacauuugu caagguguua accuucacag agucugacaa auauuucgau     3360

ggcaagaagg acaaaaucca gauauuuuuc gggaaucucc uuaagagcaa cuacaaacag     3420

gaugacucau uuuauaucag uuccaugcaa aaguacaacg ucagaaaagu gauacugacu     3480

ucucugggaa ucauuaagua caggagccag aucuugaaga auaaagaagu uuga           3534


<210>  2873
<211>  3540
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2873
augagcaaga aaguguccag aagguacgag gaacaggccc aagagaucug ccagcggcug       60

ggcucucgcc ccuauucaau ugggcucgac cuuggagucg guaguauagg cguugcugug      120

gcagccuacg auccuaucaa gaaacagcca agcgaccugg uauucguguc cucucgaauu      180

uuuaucccgu cgaccggggc ggcugaacgu agacagaaga ggggacaacg gaacucauug      240

cgccacagag ccaauagguu aaaauuccug uggaagcuac ucgcagagcg gaaccugaug      300

cuuagcuaua gugaacagga uguccccgac ccugcccgcc ugcgauuuga ggaugcugug      360

guuagagcaa auccauacga guugaggcuc aagggccuga acgaacagcu gacacucucc      420

gaguuagguu augcccugua ccauauugcu aaucaccggg ggucuagcuc cgugcguacu      480

uuccuugacg aagagaaauc aagugaugac aagaaauugg aggaacagca agccaugacg      540

gagcagcugg cgaaggaaaa aggaaucagc accuuuauag agguccuaac agcauucaac      600

acuaauggcc ucaucggcua ucgcaacucu gaauccguga agucaaaggg gguacccguu      660

ccuaccagag auauuaucag caaugagauu gacgugcugc uucagacaca gaaacaauuu      720

uaccaggaga uccugaguga ugaauacugu gacaggauag ucucugccau uuuguucgag      780

aacgaaaaga ucgugccaga ggcuggaugc ugucccuauu uuccugauga gaaaaagcuc      840

ccacggugcc auuuccugaa ugaggagcga cgcuuauggg aagccauuaa caaugcaaga      900

aucaaaaugc cgaugcagga gggugcugcc aagagguacc aauccgcgag cuuuucggac      960

gaacagcggc acauccuguu ccauauugcc cgcucuggca ccgacauaac ucccaaacuc     1020

gugcagaagg aguuuccugc acuuaagaca uccaucauug uccugcaggg gaaagaaaag     1080

gcuauccaga aaauagccgg auucagauuc aggcguuugg aggagaaguc auuuuggaaa     1140

cggcuaagug aagaacaaaa ggaugacuuc uuuagcgcuu ggaccaacac gccagaugac     1200

aagcgacugu ccaaauaucu caugaagcac uugcuucuga cugaaaauga gguuguggau     1260

gccuugaaaa ccguaucucu cauuggcgac uacgguccca ucgggaagac agcaacucag     1320

cuguuaauga aacaccugga ggauggacua accuauacag aagcccucga gagaggcaug     1380

gaaaccggcg aguuccagga acugagcgug ugggagcaac agucacuuuu gccuuacuau     1440

gggcagaucc ugacgggaag uacucaggcu cucaugggua aguacuggca uuccgcguuu     1500

aaggagaaaa gggacagcga aggcuucuuu aagccaaaca caaacucuga ugaggaaaaa     1560

uacgggcgca uugcaaaucc cgucgugcac caaacccuga acgagcuucg gaagcugaug     1620

aaugaauuga ucacuauucu cggagccaaa ccgcaggaga uaacaguuga gcuggcuaga     1680

gaauuaaagg ugggcgccga gaagagggaa gacaucauua aacagcagac caagcaagag     1740

aaagaagcag uccuggccua uucaaaguac ugugagccua acaaucuaga uaaacgcuau     1800

aucgagcgau uccggcuccu ggaagaccag gcuuuugugu gccccuacug ucuugagcau     1860

aucaguguag ccgacauugc agcgggucgu gcugauguug accacauauu cccaagagau     1920

gacacggccg auaacagcua ugggaauaag guggucgcac acaggcagug caacgacauc     1980

aaaggcaagc gcaccccuua cgccgcuuuu uccaauacau cugccugggg acccauuaug     2040

cauuauuugg augaaacucc aggcaugugg cggaagagaa ggaaauucga gaccaacgag     2100

gaagaguacg caaaguaccu gcaaucgaaa ggguuuguga gccgauucga auccgacaau     2160

ucauauaucg cuaaggccgc gaaagaguac cuccgcuguc uguucaaccc caauaacguc     2220

acagccgugg gaaguuuaaa ggguauggaa acuucuauuc ugcggaaggc uuggaaucuu     2280

cagggcaucg augacuugcu cgggagcaga cacuggucca aagaugcaga caccucaccu     2340

acaaugagga agaaccguga ugacaaccgc caucacggac uggacgccau aguugcucua     2400

uauugcagcc ggucucuggu gcagaugauc aauaccaugu ccgagcaggg caaaagagcc     2460

guagagauug aagcaaugau cccaauuccg gguuacgcga gugagcccaa ccucagcuuu     2520

gaagcccaaa gggagcuguu ccgaaagaaa auccuugaau uuauggauuu gcaugcuuuc     2580

gucucuauga agacugacaa ugaugccaac ggggcacugu uaaaggacac gguguauucc     2640

auacucggcg cugauacaca gggagaggac cugguuuuug uggucaaaaa gaaaauuaag     2700

gauaucggcg ugaaaauugg ggacuacgag gaaguagccu cagcaauccg gggacgcaua     2760

accgauaagc agccuaagug guauccaaug gagaugaaag acaagaucga acagcuucaa     2820

aguaaaaaug aggccgcucu gcagaaguac aaagaaagcc uggugcaggc ggccgcaguu     2880

cucgaggagu cgaacagaaa gcugauugaa uccgguaaaa agcccaucca gcuaucugag     2940

aagacuauua gcaaaaaggc ucuggaacuc gucggcgggu acuauuaccu gaucucaaau     3000

aacaaaagga ccaagacauu cguggugaaa gagccuagua augaggucaa gggauuugcc     3060

uucgauaccg gcuccaaccu uuguuuggac uuuuaucacg augcccaagg uaagcugugc     3120

ggggaaauaa uucggaaaau ccaggcuaug aauccaucuu acaagcccgc auauaugaaa     3180

cagggcuaca gcuuauaugu ucgacucuac caaggagacg ugugcgagcu gagagccucc     3240

gaccuuacug aagcggaguc aaaccuggcu aagacaacgc acgugcgccu accgaaugcc     3300

aaaccuggca ggaccuucgu aaucauuauc acuuuuacag aaauggggag cggauaccag     3360

auuuauuuca guaaccucgc aaagucuaag aaaggccagg auaccuccuu uacacugacu     3420

accauaaaga auuacgacgu ccguaaagug caguugagcu cggccggucu gguucgguau     3480

gugucaccau uacucgucga uaagaucgag aaagacgagg uggcucugug uggggaauga     3540


<210>  2874
<211>  3567
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2874
augaaguaca uccugggccu cgacauuggg gugagcuccg ucggaauagc cguuaucucu       60

gaggaaaacc agaaaaagac cauugagaau cuugcuguga gaaucguacc cgaagauccu      120

gacuuccacg guaaauuuua uucaggcaac acagcaagua agaaucugga gaggacugcc      180

aaacggggga accgcaagaa uaaccaacga uucaaggcgc guagagauaa auuguaccag      240

agcuuaaagg aaaauaacau guuuccaacg uccaaucugu ucgaccuauc ugcuguggag      300

cucuauggac ugagggccaa agcagucucg gaacaggugu cacuucagga acugggccgg      360

auuuugaucc uccugaacca acgcagaggu uuucugagca auaggaagag uguuuccgag      420

gaaaacucua ccgaguacaa agagcggaua gccgggcucg aaaaggcuuu aggaaauaag      480

acaaucggcc agaaacugua uagcgagcuu caggaaaaca auaacguguu cgaggucuug      540

cugcgcgaac gaacuuacca gagauccuca uauaccgagg aguuugauag gauuugggac      600

gaacaaaaga aauacuaccu aauccucaca ggcaguagca acgaggauga caauaagggg      660

acccuguaug aucuuauucg gaaccguauc auauacuauc agcgcccgcu gaaaucucag      720

aagggauuga uuuccgaaug ccucuucgag aaacaucaca aggcaguggc caagucaagc      780

cccuacuuug aacuguucag aaucuggcag aaaauuaaug acuuaaguug gaagacuccu      840

aacggugagc uguuuaaacc aacagaugag caaaagcaga aacucaagaa ugcucuuugg      900

aacggcgaca aucugaaccc caagcauaaa uugaccguau cuaagaucaa agaaauccua      960

ggguauacgu ccagggagaa gauuuaccug aauuucacug aacucgaugg aagccggacc     1020

uauucgauac ugcgaaacgc ccuuaucgag gcgggcauug aaaaaccuga gcaguaccug     1080

uuuuucaaua uggagacaaa cgacgaaaag gguggguugu ucgagcucug gcacaucacu     1140

uacucucugc caauagaaaa ggagguuauu aaugcauuaa agaaccgcuu uggauucgac     1200

aagaaacaag ccgagaucau cgcuagaagg gugggcuauu ccucagauua cggcagucug     1260

agcacccggg ccauucgcaa gcuacucguc caucuggaaa aagggcuugg auauacagac     1320

gcaugugaua auaucgguua cgaccacucc ggcuauaaga ccaagauuga uuugcagccc     1380

aaacugaagc agcucaaaag aaacgagcug aggaacccug ugguggaaca gauacuuaau     1440

caagucguua acgcugugaa ucugaucauu gagaaguacg ggaaaaucga cgaaauccgu     1500

guagaguugg cccgggagcu ccgaaacaau gcgaagacga ggaaaaacau uucucuguua     1560

aauagcaaga acaagaaaaa uaacgauaua aucagaaaga aacugcagga agaguaugga     1620

uuuaaguucg ugaauggccg cgacgucacu cgguacauuc uaugggaaga gaacucaaaa     1680

gaaugccucu acuguaauaa gacaaucaac gguagugaua ugcugaccgg gaaugccgac     1740

auugagcaca uccuuccaaa guccaggagc uuuaacaacg caaugaauaa cuauauaauc     1800

acucauagaa aaugcaauuc ugauaagggc cagaaaacag cuuacgacuu caugucaagu     1860

aagggagagg ugauuuugca gcaauauauc gaaaaaguua acgaccugua caaggauggc     1920

aaggggagca uuuccaaaac caaguuugag aaucuccugu guaaaggaga agacaucccg     1980

ucugauuucg uggagcgcau gaagaaagac ucgcaguaua uagccaagga agcugucaag     2040

auguuaaaaa cggugugccc caacguauac accacaacug gucagguuac cgauuuucug     2100

cgagaggagu ggaaacuuaa aaauuugcuc caggaacuga cauucgagaa guauaaagcc     2160

auuggccaag ugaaggagaa aaucauuaag acuaacaaug ggcagaccaa gacauuuaaa     2220

gucaucgcag acuggagcaa gcgggaugac caccgucauc acgccauaga ugcucuaauc     2280

ugugcgcuga ccgaccagaa aauuaucuuc aagcucaaca aucugaacaa aauuuaccag     2340

uacgagagaa augcacuuuc ccaaaaggag aucgaagaga uagaagaguu uuuggaagga     2400

aaguucucaa uuaaagaguu cagugcccag cagggcaaca ucaucgagug cucuauuccu     2460

aauaucagga aggaugcuca aaaacaccug gaaaacauuu uaauuagcuu uaagaaagag     2520

aacuccaagg ugcucacuau gaaucgcaac cagccaaaga auuguaaaga acagaucacg     2580

uggguccccc gggccagacu gcaugaggac acaauuaugg guagggugaa gcgaaucccu     2640

gagaaaaaga ugaaacuuaa cgauaagguu ucauucgaac agauuaagau gaucgugaau     2700

ccagagauaa aagaaauccu guuguaucgc cucgcaaagu uuggggacaa ccccaaagua     2760

gccuucgcua ccaaggcccu gaaaaaggag ccgauauuuu accgggaaaa ugagcuaaag     2820

gaagucagca ucuaugagug ggugaacacu aaaagaguug cacugaauga gaacauuacc     2880

gacucucaaa ucgaaaagau aauugaucag gcguccaaaa gggcugugaa ggagcgucuc     2940

aauggcggaa aaaucaagga agucuucaaa aaccugacag agaauccuau ccagcuuaag     3000

aacggcauga uuuugaagaa ugugaccgua acugacgaga guaaagugga aaagauacgc     3060

aacggguuug ccaucacagg uaaaaaucac caugcacugg uuuacaagga ugagaacgga     3120

aaauauaacg acaaggucgu gagcuucugg gaagccgugg cuauuggcuu acggaauguc     3180

acggaguacg ggaaccccua uccaaucauu aauagaaacc acaaugauga acucggagag     3240

uuuuacuucu cuaugcagau caacgaccug uuuguuuucg aucuuaagca uuccgagaau     3300

ccucaagaag agaacgaaau aaacuuuuuc gacguggaga auaggaaaca gaucucagau     3360

aagcuguucc gagugcagaa aaugaguaag aaaagcaaug gccaguuugu aucggacuuc     3420

cggcaccacc uagaaaccuc cgucauucgc aacgauauga uccucaagaa uauuacuugg     3480

aucucuauaa gcaagaacuc agaccuggag agauugacaa aaauuagggu gaaucaucug     3540

ggugagauca ucaagguugg ggaauga                                         3567


<210>  2875
<211>  3585
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2875
auggacuuca ccgccgaugu gagcgagcug aacgaauccu uuaugcagau ccacacagag       60

ucuaaguucg aaauugacac ugcuaaaguc caagagauau cacagcuccu ucuggauaau      120

aagaucacga gaauggacag gcagaaacag guugcaaacg uguuggccaa gaauaguggc      180

gauaaagaag cggacaagau uaacaagaaa aucgcuaccg aguuuugcaa ggccguauua      240

gggaauaaag caaaguucaa cgugauucug ggagaggaug ccgucgacag cgauuggucc      300

uuuaaaaugg acgaugaaaa uucugacgag gaaaucgaga uacuaauggg ucaaacaaac      360

ggccagcagc agaaugugcu ccggaucauu caucaauggu acucgcagau cacucugaac      420

gggauuguuc ccgauggaca gaugaucuca gagagcauga uagacaagua ugaacuucac      480

cgcaagcauc uguugcuccu gaaacagcaa auuaagguga ccaaagacaa gaaaaaggcu      540

cagcagcugc acgaggcaua cgaacaauau guccauggca caggugccaa aaagaagcuc      600

acccaggaug acuucuacag uaaagugacu aagaauuuag agacauccga uuuuucugac      660

gaaaucaaaa agcagauuaa cgcugagacc uucaugccua aacagcgaac gagcgccaau      720

gggguaaucc cacaccaacu gcaccagcag gagcuugaua agaucauuga aaagcaggcg      780

aaauauuacc cguuuuuggc agagcugaac cccgacacua agcgucaaaa uauagccaaa      840

uauaagcuag augaacucau cgcuuucaaa auuccuuacu acguuggacc acugaucacc      900

acagaggacc agaagaagac uuccggcaaa ucauuugccu ggauggugag aaaggaaccc      960

ggcaaaauaa ccccuuggaa cuucgaucag aaggucgaca aaauggcaag ugcugaucag     1020

uuuauuaggc ggaugacaac caaggacagc uaucuuauca cggaggacgu gcugccagcc     1080

gagucuuuga ucuaccaaaa guauaaagug cucaacgaac ugaauaugau ucgcgucaac     1140

gggaauaagu uaacuaaaca ugauaagcag aacaucuacc agcagguuuu cgagaaauau     1200

aagacaguga auguaaagaa ggugcaaaac uacuuccuga ccgacggaag auaucagcac     1260

gcgcccaaga uuacuggucu cuccgaugac aauaaauuua acucaaaucu uagcacauac     1320

auagauuuca agaaaaucuu ucaggacguc guggaugacc cucaaaagca gaaagauuuc     1380

gaaaggauua ucgaguggag uaccaucuuu gaagaccggc auauuuacga gguuaagcug     1440

aaggagauau cuugguugaa cgaugaacag cgcaaagagc uaaagaaaaa gcgacaccag     1500

ggcuggggga gacuguccgc caaacuccug acgggaaucu ucgacgaaaa uggcuauagc     1560

auuauggauc aacuuuggaa cacccagaag aauuuuaugc agauccaggc agaggagggu     1620

uucaacaaga aaauucaagc ugaaaauucg aagcaccugg agaaaaacga cuuugaagac     1680

aucuuggagg augccuacac aucuccacag aacaagaaag cuauaaggca agugaucaag     1740

gucguggaag acauucagaa gacuaugggg accgccccgc agaaaaucuc cauugaguuc     1800

acacggucag cagauaagaa uccccgucgc aguaucgaga gacagaggca gcucgaagag     1860

auauauuaca acgacgccaa agaaauugua agcgagggaa ugcaagauaa ucugcagaac     1920

uuuacugaca auaagcagac cuuaacagau aaauauuucc uguacuuuac ccagcuaggc     1980

aucgacaugu auacuggcaa gaaaauuaac aucgaugaaa uagggaauua ccaaaucgac     2040

cauauucucc cucaggcuuu caucaaggau gacuccauug auaacaaggu ucuggugucu     2100

agcgcgauca auaacggaaa aucagacaau gucccacuua guauguacgc auccgugcgg     2160

auaaagagcg agguccagaa caaauggaag aauuucgccg agcacgguuu gauuucucag     2220

cgaaaacuga acaaucucau gacggacccc gaaucaaucg auaaguauaa ggcucgcggc     2280

uuuaucaaaa gacaacuggu ggagacaagu cagguuauua ggcuuguggc cgaaauccug     2340

aacgagaagu accaggggga aaccgagauu auagagguac gggcaaaaau gaacagccag     2400

uugcgcaagg acuucgaucu ccugaaaaau agaaacguca augacuauca ucacgccuua     2460

gaugcuuacc ugacuaccuu uaucggaaac uaucuauaca ggcguuaucc uaagcuccgg     2520

uccuacuuca cauacggcca uuuuaagaaa auugacgaug ccaagcugaa acaauucaau     2580

uuucuucacg acaucgaaaa cgauaaggac ccaaaauaug ugaaugauaa cggugaguug     2640

cugugggacc gagaaaagga cauugcauac uucaagaaaa ucuauaauuu uaaguacaug     2700

cucguuucuc acgagguguc gaccagacag ggggcgaugu ucaaacagac uauauuuccc     2760

gcugccgcag augccaagag caaacugauc ccgauuaaga acgacaagcc uacagcuauc     2820

uauggcggau acacgaauaa caaugaugcc uauuuagcaa uuaucaaacu ggcuaagacc     2880

ggccaguuca aagucguggg gguaccccaa agggccauag ugaaacuuaa acaggcguac     2940

gacaaggaga cuggagauua cuccaacguu uuguauaaag uccucgaacc aaaguuugug     3000

aagaaaaagg ugaaucgcaa aacaggugag auuaccuacc aggucgaaaa guucaaaauc     3060

guugugccua aggugcccua ucggcaacug guaaucgacg gcgaucagaa guucaugcua     3120

gggucaagua cauacaaaua uaacgccaag cagcuggucc ucucugagaa cgcuauuaaa     3180

auacuggaca agaaauccuu ugaucagagc ggauacaagu caagcgaccu ugauacugug     3240

uaugacgaaa ucuugucuca aguugauaga uacuucucca uguacgacaa gaauaaauuu     3300

agggagggcc ugcauaacgg uaagaaauau uuccagcgcu uaccaaugau ugacaaugug     3360

gagaagggga aaaucaagga aaacggcaag uuuaaacucc ugaaugagau ucuuaucgga     3420

cugcacgcaa accccaguuu cggcaaucuc aagagcuugg ggauaucuac cccuuuugga     3480

augaugcagu ucgccuccgg uaucucacug agugaaaacg cuauucuaug uuaccagagc     3540

ccaacgggca ucuuugagcg acgggucgcc cugaaagauc ucuga                     3585


<210>  2876
<211>  3588
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2876
augcagaaga acaucaauac caaacaaaac cacauuuaca uaaagcaggc ccagaaaauc       60

aaggagaaac ugggcgacaa gcccuauaga auugggcucg aucuuggagu ggguagcauc      120

ggcuucgcua uugucuccau ggaagagaau gacgggaacg uucuguugcc uaaggaaauc      180

auaauggugg gaucuaggau cuuuaaagca ucagccggcg cggcugaucg gaaguuaagu      240

cgcggucagc gaaauaacca ucgucacaca agagagagga ugcgguaccu guggaaagua      300

cuagccgaac aaaagcucgc acugccagug ccggccgacc uugaucgcaa agagaauagc      360

uccgaggggg aaacuucugc uaagagauuc cugggagacg ucuugcagaa ggauauuuau      420

gagcucaggg ugaaaucgcu ggacgaacgg cugucacucc aggaguuagg cuacguucug      480

uaucauaucg caggccaccg cgggagcagu gccauucgaa cguuugagaa cgauuccgaa      540

gaggcucaga aggaaaauac cgagaacaaa aagaucgccg gaaauauaaa aagacuuaug      600

gcgaagaaaa acuacaggac auauggugaa uacuuguaca aggaguucuu ugagaauaag      660

gaaaaacaua agcgggagaa aauuucuaac gcagccaaua accacaaguu cagccccacu      720

cgugaccugg ugaucaaaga agcugaggcc auucuaaaga agcaagcagg caaagauggg      780

uuucacaagg aacucaccga ggaguauauc gaaaaacuga caaaggcuau cggauacgag      840

uccgaaaaac uuauuccuga gucaggcuuc ugcccauauc ugaaggacga aaagcgcuug      900

cccgccaguc auaaacucaa cgaggagaga aggcuguggg aaaccuuaaa uaacgcgcgg      960

uacagcgauc cuauagucga caucgugacu ggugagauua caggguauua cgaaaagcag     1020

uuuaccaaag agcagaagca gaaacuguuc gacuaucucc uuacgggauc ugagcugacu     1080

ccagcccaaa ccaagaaguu gcuaggccug aaaaauacaa acuuugaaga uaucauacuc     1140

cagggccgag acaagaaagc acagaagauu aaaggguaca agcugaucaa acuugagucc     1200

augcccuucu gggcucgccu gucagaagcc caacaggaua gcuuuuugua cgacuggaau     1260

aguuguccug augagaagcu ccugacugaa aaguuaucua acgaguauca ccugaccgag     1320

gaagagaucg acaaugcuuu caacgaaauu guacuaucca gcucguacgc cccacucgga     1380

aaaucugcaa ugcugaucau ucuugagaag auaaagaaug auuuguccua uacagaagcc     1440

guugaggagg cucugaagga agguaaacuc accaaggaga agcaggcgau caaagacaga     1500

cugccguacu auggcgcagu gcuucaggaa ucaacgcaaa agauuaucgc caaaggguuc     1560

aguccccagu uuaaggauaa aggauacaag acuccucaua caaacaagua ugagcuggaa     1620

uacggcagga ucgcuaaucc agucgugcac cagaccuuga acgagcuccg gaaacuggug     1680

aaugagauua uagacaucuu agguaagaaa cccugcgaaa uugggcugga gacugcccgc     1740

gaacuaaaga aaagcgcaga ggauagaucc aagcucucua gggagcagaa cgacaaugaa     1800

agcaaccgua accggaucua cgagauuuau auccgaccuc aacagcaggu cauaaucaca     1860

agaagggaaa auccacgcaa cuacauucug aaguucgagc uuuuggaaga acagaaauca     1920

caaugucccu uuugcggcgg acagaucagu ccgaaugaca uuaucaacaa ucaggccgau     1980

auagagcacc uguucccuau ugcugaaucc gaggacaacg gccggaauaa ccucguuauc     2040

agccauucug ccuguaaugc agauaaggcg aaaagaucac ccugggcugc cuuugcaagu     2100

gccgcuaagg acagcaaaua ugauuacaac aggauucugu ccaaugugaa ggaaaacauc     2160

ccacacaaag ccuggcgcuu caaucagggg gcauuugaga aguucauaga aaacaagccu     2220

auggcugccc gauuuaaaac cgacaauucu uauaucucga agguagcgca uaaauacuua     2280

gccugccugu ucgagaagcc caacauuauc ugugugaaag gaagccuuac ggcucaauug     2340

cggauggcau ggggucucca gggccugaug auuccauuug ccaagcagcu aaucaccgag     2400

aaggaauccg agucauucaa caaagauguc aauaguaaca agaaaauacg ucuggacaau     2460

agacaccaug cucucgaugc cauugugauc gcauaugcgu cuagggggua cggaaaccug     2520

cuuaauaaga uggccggcaa agacuacaag aucaacuaua gcgaacgcaa uugguugucc     2580

aagauucugu uacccccuaa caauaucguu ugggagaaca uugaugcuga ccucgaauca     2640

uuugagagcu cugugaaaac agcccugaag aaugcauuca uauccgucaa acacgaucac     2700

agugacaacg gugagcuugu gaaggggacu auguacaaaa ucuucuauag cgaacggggc     2760

uacacccuga caacuuauaa gaaguugucu gcucucaaac ugaccgaccc acaaaagaaa     2820

aagacaccga aagauuuucu agagaccgcc cugcucaagu ucaagggaag agaauccgag     2880

augaaaaaug agaagauuaa aucagcaauc gaaaacaaua agaggcuguu ugacguaauu     2940

caggauaacc uugagaaagc caagaaauug cuggaagagg aaaacgagaa gaguaaggcu     3000

gagggcaaaa aggagaaaaa uaucaacgac gcgagcauau accagaaggc caucucguua     3060

uccggggaua aauauguuca gcucucuaag aaggagcccg gaaaauucuu ugcaauuagc     3120

aagccuacuc caacgacaac cgguuacggc uacgacacug gggauucacu gugcguggac     3180

cuuuauuacg auaauaaagg aaagcugugu ggcgaaauca uucgaaaaau cgacgcucaa     3240

cagaagaacc cccuaaagua uaaagagcag gguuucaccc ucuuugaacg cauauacggg     3300

ggcgauauuc uggaggucga cuucgauauc cauagugaca aaaauuccuu ucggaacaau     3360

acaggaucug ccccugagaa cagaguguuc aucaaagucg gcaccuuuac ugaaauuaca     3420

aauaacaaua uacagaucug guucgggaac auuaucaaga gcacgggagg ccaagacgau     3480

uccuuuacca uuaauucaau gcagcaguau aacccaagga aauugauccu gagcaguugc     3540

gguuucauaa aguaccguuc ucccaucuua aagaauaaag agggguga                  3588


<210>  2877
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2877
augagcaagc acgugcuggg ccucgaccuu ggggucggau ccaucgguug gugccugauu       60

gccuuggaug agcaggacca acccgcugaa auauuaggca uggggucuag aaucguuccu      120

cugaccacag augaccuaaa cgaguucuca cagggaaaag caauuaguaa gaaucagaaa      180

aggacuacgc agcggaccau ccgcaagggc cucgaucgau accaacugcg uagaaaagug      240

cuuagcgaaa agcuggccgg ucaugggaug uugccagaca aggaucucau gcagcugccg      300

guacuggacc ucugggaguu aagggcgcgg gcugccuccc ccggagaaaa acugucucuu      360

ccugagauug gccgcguguu guaucacauc aaccagaaga gaggcuacaa acaugcaaag      420

ucggaugagg ccgaagaggu cacaaaaagg gggaaggaac agggauaugu ggcuacuguu      480

aagggucggu acgcagagcu gcaacgcgag ggcgccaccg uggggcagca cuuugcuugu      540

aaacuaaagg aacaucgaca ggagucacca agcggaggcu ucuuuuauaa uuacagaaua      600

aaagaccagg ucuaucccag ggaagccuac aucgaggagu ucgaucggau uauggaagug      660

caaaguguau uuuacccuga gguucucaca ccagcguuca uccaggaacu gcgugacaag      720

gugaucuuuu uccagcgccc ccuuaaaucc ugcaaggguc uggucucuuu uugugaguuc      780

gaaaaacaag agagaguggu gcaguugaag agggucaacc gggaagggaa ggaggagcuc      840

caggaacgaa aaauuaccau aggaccuaag guugcaccaa aaagcucccc gauugcccag      900

cugugcaagu uaugggaguc aauccacaau auucgcaucu auuaccccga uggcaaagaa      960

agaccuauca cucuggagga aaagaggaag cuccuugcuu aucugcaaac acacgacaaa     1020

uugaccuuua cggcccuaaa gaaagagcug aagauuagug acaaagagcg gcucuggugu     1080

gauaagcugc uuaagggcgg gauaaaagga gacacuaccc gcgugcuguu gagaaaggca     1140

cucgcugguu acaaaaggua ugaaccacug uuacguaugg aucuggagcu acgggacuuc     1200

caggaaaucg uaagcggcaa gcgacucauu agacccaucg uggaugaguc uuaccugucc     1260

cagccucuuu auagguugug gcauauacug uacucaauug aggaacgcga ggccaugcgg     1320

agagcgcuca ucgaccagcu gggguuucuu gaagaggauc uggacggagg cuugcucgau     1380

aaacuguacc aaaucgacuu cgccaagcag gguuauagca acaagagugc aaaauucauu     1440

ugcaaguuac ugccacaccu acaagggggc cauggauacu cucaggcuug ugaacucgcc     1500

ggcaaaaggc acuccgcugg gagccugaca aaggaggagc uugaggaacg ccccuugcug     1560

gaacacaucc cgcuccugca gugcaaugag uuacgacagc cuauugucga aaagauacug     1620

aaccagauga ucaaucuugu gaacgccuug cggcagaagu auggaucgau ugaugagguu     1680

cguguggagc ucgcaagaga acugaaaaug ucuagggagg aacgcgagga aacuuccaag     1740

cggaucagau caagggagaa ggagaauaaa gaaaucgccg agcgaauuaa ggaauacgag     1800

cuauauccca ccaaaagucg cauacaaaag uaccggcugu ggcaggaggc uggugaacag     1860

ugucucuauu gcggcagaag caucgacguc gugcaauuuc ugcgugggga cggaguagag     1920

auugaacaua ucauuccaaa auccauccuu uacgaugacu cuagcgcgaa caagacaugu     1980

gcaugcucaa ggugcaauaa ggauaaaggc aaccgcaccg ccuacgagua uauauuggcu     2040

gaaggucggg aggccgagua ccuggacaga uuagaaacuc ucuuccacga ugggaagcug     2100

aguuauucca aaaggcgacg gcuucagugg aagaaagcag acaucccugu ugauuuuauu     2160

gagcgccagc ugagauugac gcaguacauc gccaggcaag cucaggaaau ucuccggcag     2220

ggcauccgaa gagugggagc cagcgagggc ucugucacag caaagcugcg ccaucuaugg     2280

ggguaugacg auauacugag ggaacucaau uucgagcguu accagucaau gggagagacc     2340

gaaauuguga gucaagagga aaaggacggu agcaucacua aauccgagcg gauuaaggau     2400

uggucuaaaa gaaaggacca caggcaucac gcgaucgaug cucuggucgu ggccuguaca     2460

cgccagggcu auauacagcg acuuaacucg uugaacagcg aagaggacuc ccugcagaug     2520

caagaggacu caaaacagau gagagaaaag augggagggc agaagcggcc caccgcagau     2580

aaacacagua ucuuagagaa uuggcucauu cugcaaccac auuuuucuca ccaggaaguu     2640

aagagcaaag uggccgagau ccuuguaucc uucaggcccg gcaagcgcac ggucucaacc     2700

ggucguaaca cauaccugag aagcgggaaa aaggugacuc agucuggcgu ucuaauuccu     2760

cggggaccac ucuccgagga aagucuguac ggccagaucu ugaggaaugg ggagccgacc     2820

uaugugaaaa aggucgcucu ggagagcuua ucugaagccc aaauagaaca gauuguggac     2880

cccaagcucc gccgagagau gguagcaaga acacagccuu ccgagggagc uaaacugaac     2940

ccaaaacuuc uguuggccac ucccuuuuac ucagaugcgg ccggcaccca gcgggugagg     3000

aguguucgcg ucgcuaucga aaaugguaaa aacagcaucg ugccucucag auauaaugag     3060

ucggggaagg caauuacauu cgugaaccca ggaaauaacc aucacgucgg caucuacagg     3120

accuccgaag guaaacuggu ugagucuugc gugacuuuuu ggcaagccgu ggaccgggua     3180

cguuauggca uucccacgau aauccgcaag ccgcgagaag uccuagauca gcugcuucuc     3240

agagacgaua uuccugaggc ucuguuggcc caucugccag caagcgacug ggaguucgug     3300

gaauacuuac aggcgaauga gauguuucuc aucgggcuga cagaugaaga gauucagacc     3360

gcccuugcug aaucagaccu guugacucuc aguaaccacc uguauagggu ucaaaagaug     3420

uccucugagu acuacguguu ccgguaucac cuagagaccg aagucgccga uaauaaaaac     3480

aauacaggaa ucauacccaa guuuuaccgc cugagaggcc cuaacgacac cuaucuuaaa     3540

gcaaacccca ggaaggugaa aguuaaucuc cugggggaga ucagcuugcc auga           3594


<210>  2878
<211>  3615
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2878
auggagagaa uccugggcau ugacaccggg acaaacagcc ucggaugggc cauaguggau       60

aaggacgaaa aucaguacca ccuugucgcu aaagguacua acaucuucuc cgagggcguu      120

aagauugaaa aagggaucga gucuucaaag gcagccgaaa ggacggagca ucggagugug      180

cgcaaacacu auuggcgacg uaagauuaga aagaucaggc uguugaccau auuaagcgau      240

aaugaccugu gcccccggcu aaaacaugag caacuccgcc aguggagacu gaagaaagua      300

uacccuacag augacacuuu uauggaaugg cagaggaccg aggauaaggc ucaggugaac      360

ccauauaaau uccgguacau cugucuuaca caaaagcugg acuuguccga uauuacccag      420

cgcuuuaucc ucggacgagc gcuguaucac cugaaucaga gaaggggcuu ccucucuaac      480

cggaaggaag ccacuaaaga gucggagggu acagucaagg aagggauuuc aagccugagu      540

aaagacauga aggauuccgg augcgaguac cugggcgaau auuuuuacca gcuuuaccaa      600

cagggcaaaa agauccguaa ucauuauacc gcacgcaaag agcacuacuu gaaggaguuc      660

aaggccauau guucuaaaca ggaacuggac gaugcucuaa uugagaagcu cgaaaaagca      720

aucuuugacc agagaccguu aaagagccaa aaaggacagg uggggacgug cacuuucgag      780

aaggguaagu ccaggugucc cucaagucac ccucuuuaug aagacuuucg gauguacagc      840

uucauuaaca auaucaaagu ucagguggau acccuguaug acaagggcga gcgaucuuug      900

acagaugaag agaaacaacu caucauucca cuguuuaagc gcaaauccaa gagaacuuuc      960

cccuuugagg acauagccaa gaaaaucgcu gggaagaaca auuacugcua uauuaaauca     1020

aacgaagaga aggccuacaa auucaauuac cagauggaua cccaggucag cggaaguccu     1080

gugaacgcgc aguuagaaga gaucuucggc gaagacuggc ugucuuccgu augugagguu     1140

uauacacucg caaauaacaa gagcagguuu caaauaauga acgaugugug gcauguccuu     1200

uucuuuuacg acgaugagga caagcugaaa gaguucgcca aggaucgguu gcagcuagac     1260

gacgaacugg cugaaaaauu uucgaagauu ucuaucccaa augauuaugc cgcacucucc     1320

cugaaagcua uccgcaagau uaugcccuac cuuagagacu auggucugau cuacucagag     1380

gccguguucu uggcgaaccu cguggauguc cugccucagc acguuugggg gauuaaagag     1440

augagggaag ccgcaauaga gaaugugauc gacguaaugc auaguuauga uaagaacaau     1500

auugacggaa ucaccuuaga aggcugcgug aagacguuuc ugaaagagcg uuaccagguc     1560

agcgaugaca accuaaagaa acucuaccac ccauccaugc uggauccgua uccccggcaa     1620

cgaccuaaug acgaaggcgu guuccagcuu gggucuccaa gaaucagcuc aguuaagaac     1680

cccauggcua ugcacaguuu guuuaggcug cgcaaagugg ucaaucucau gcugaagaag     1740

ggauacauua cuccugauac aaaagugcau auagaguucu cccgggaccu uaacgaugcc     1800

aauaagagau gggcuaucca gaccuggcag agggagaacg aaaaagagcg cgaacgaugu     1860

cggaaggaga uugaaaaaua uaucgccagc ccaucugaca cugacauucu gaaguaccaa     1920

uugugggagg agcagaauca caagugccuc uauacaggug augaaauccg uauaaccgac     1980

uuucugggcg auaaccccaa auacgacauc gagcauacga uuccgagauc agcaggggga     2040

gauaguacca agaugaauuu aacacugugu agcuccaggu ucaaccgcga cgaaaagaag     2100

acuaaacuac cugccgagcu cguaaaccac gaugaaaucc uggagcggau uucuaauugg     2160

aaggagaagu augaagacau cgauaaacag auaagaaaga uuucgaccaa aggcgcuaca     2220

agcaaggagg acaaagauuc caagaucagg cgacgccauu accuuauuuu gcagcgggac     2280

uacuggagag guaaauauca aagguuuacu augaccgaag uucccgaggg guucucacgu     2340

cgccagggca cagauaucag ugugauaucu cgguacgcga gaauguaucu caagagcguc     2400

uucaaacagg uguacaucgu caaaggaauu gcaaccuccg acuuuaggaa gauguggggc     2460

auccaagacg aguaugccaa aaaggaacga gugaaccacg uucaccauug cauugaugcu     2520

aucacuauag ccugcauugg gaaagcagag uacgacaagc uggccgauua ucggcaccag     2580

uacgacgauu accuguucca gggaaagacg aaaccaacau uugacaagcc uuggcccuca     2640

uucgugaucg auaucaaaca uauucaggac gaauuacuga ucgcucacua ugccaaggau     2700

aaucuugaga aauuuaccaa gaagaacuug cgcgguaaag acggcagaau uauaagggau     2760

aaguucggga auaaacucca aaugaaggga gacacuagcc ggaacucucu gcacaaugac     2820

aaauuuuacg gcgcaaucaa ccaugaaggu gagauuaagu auguauaccg aaccaauauc     2880

gaggcgcuag augcuaaguc cauugggaac aucgucgacc caacagugag aaguauaauc     2940

gaaagcgcca uugcaaucca cggcucucug aaagccgcua uuaauaaggg aaucuggaug     3000

aacgaggaaa aacauauacc cauuaagaaa guucgcgugu ucaggaauga ugucaagaag     3060

ccuaucgagc ucaaagacca gcgugaucug uccgacaagg auuauaaaca gcacuacuau     3120

gugaccaacg acggcaauua caugaugcuu aucuuuucaa aguugaacaa caaagggcgg     3180

gaaauuagac agcauguagu gauaaauagu cuggaggcca cugagguuau caagagccac     3240

aaaaggucgc aagucggaaa gguggugugg gaauccuacu auucuaagga ggguuucgau     3300

uuaauuuaca aacucaagag cggcacaaug auccugcuuu augaaaacaa ucgcgacgag     3360

gucugggauc uggacauucg aaacuugcag aaaagacucu acaucauaac gaagaugaaa     3420

uaugaugggc ggaucuacau gaugcaccau caggaagcaa gggagaagaa ggaccuggag     3480

gaaaaaucag gaaguuuuaa gauuaaugag gacuaucgcc cacuaaucgu uaacuccuac     3540

aaucaguuca acgcucuggu gcaagaucuc gacuuugaaa uuaaugaucu gggcgagauc     3600

cguagacuua uauga                                                      3615


<210>  2879
<211>  3630
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2879
augaagaaag acuacauccu gggccucgau auugggauaa gcuccguggg auggggucuu       60

cuggaguugg acgauaacaa uaaccccuau aagaucauug acgucggcuc uagaaucuuc      120

accccugggg aaguugagaa aacaggagau ucaagggcca aggaacggcg cgagaaacga      180

ggcgcucgua gaauugcaag gcggcgcgaa uuuagauuag acagggugaa gaaucugcua      240

uacgagaacg guuaucucaa ggggaauauc aacaguaaua uaguaagcga gaaaaacgaa      300

gaacugucca ucauuuucaa uaacaugauc aauaacuacu auaauggaaa gaacacuaau      360

ccauacauuc uuaaagugga agcccuggau cggaaguuga cggacgagga gcucucuauc      420

auacuggucc acuaugcgaa aaagcgcggc uacaagucga accgagaaga ggcuucagau      480

aaagaaagcg gcaaggugcu gaccgccauu aaagagaacg aaaagaucau gaaagacaau      540

aacuacagaa caauuaguga gauguauauc aaggaugaca aauuuaagaa uaagaucaaa      600

aacuccccga augauuacaa gauuucuguu acuaacgaga uguaucucga agagauaaau      660

aaaguguuag acagccagau caaguucggg cugauuaccg acgauuuuaa aaaccaauac      720

cuugaaaucu auaauuccag gcggucauac gcaaagggac ccgguggcaa caguccuuau      780

gggggagacu ugauagagcg uaugacaggc aagugcaauu ucgaugguaa cccacgcgcc      840

cccaaaagag cuuuuagcuc ugaaauuuac gucgcacuga ccaagcuagu gaaucucaaa      900

uacaagguaa acgacgggga guauaucaaa cuguccgaga aggaaaucaa gaauauuauc      960

gagaaagcca aggaaaagaa gucaauuagc uacaaagauc uuacuaagau gauaggaaac     1020

aacaucgacu ucaaggaucu gacauugagu aaaaaggagu auauuaaacu caucgaggag     1080

cugaagaaaa aguuaaauau caaaaaggac accaagauua acauaaauga gcugucuucc     1140

gaagauaaag agcucuacaa caaacuuugu aaugacaaac uguugagcaa gacgcuaauc     1200

gaacugaaag gcuaucauau ucucaaggau ucgguuauca aggcuuacuc uaaagacauu     1260

ugggacgagg ugaaggauaa cauguaucug cuugacgauc uggccuugua cugcacuaau     1320

uacaaaguca acgaggacau ccucaauaag auaaaagaau ccgagaacau cgauaauauu     1380

uuuaacgaca uccacuucgu ggaacuguua ccuaauuuua aggaucaucu gaugcuauca     1440

accgagcuca uuaggaagcu gaucccaaua auguauggcg ggaacacaua cgacaaagcg     1500

augaauauuc uuggauauaa ccacagugau aucaacggug acaaggaaaa gaaggauuug     1560

cuggugccca uuugugucga cgauaauauc acuaaccagc ggguuauacg aagccucacc     1620

cagacacgca aagugaucaa uuccauuauc aagaaguacg gcaugccuaa aauuaucaac     1680

auagagaccg caagagagcu ggccaagucu aggcaggaac ggaaugagau ugcuaaaaac     1740

aauguagaaa agcaagccga gaacgagaaa aucaagaauc agcuugugga acugggguug     1800

uucgucagca cggacaaaau cucaaguacu gaccuccuga aguauaaguu auggaaagag     1860

cagaacgaau uuugcggaua cuccaugaag aaaauuagca ucgaggaucu guucggcaau     1920

aacauugugc agauagacca uauccuacca uauucucgca cauacaauga uaacuaccuc     1980

aauaagaccc ugguuuauac uaaagaaaac caagagaagg guaacagaac accguacgaa     2040

ugguucggga agaccgacaa augggaugag uaugagacgu acauuaauaa ccuuaccauc     2100

ucacagaaga aaaaggacaa uuauuugcug aaaaagcucg auuuugacac agaaagggag     2160

augcguaguc agaaccugaa ugauacuaag uacauuagca aagaauuauc cucucugauc     2220

aaggcauauc uuaacgugga gaaagucaau auguaccccg gcgcuauaac cgccaaguug     2280

cgggcgcgau ggggauucaa cagacucaca cacucguacc ugagcaaaaa uuauauccua     2340

ccuaagaaca uuaaggagga caucaauaaa gauagggaca accaucugca ccacgccaug     2400

gaugcacucg ugauugcuuc caucacucca ucacugcagc aaaagauaac ccuuuacgaa     2460

aaauuuaguc gcuauauuga cggcuugaca gagcugcaga ucaagaauau cucuuacauu     2520

aaaauagaag acagcgaggg gcaguauuac aacgaugaca ccgaugaagu agagacuauc     2580

gacauucggg aguauaucaa gcaacaguua gccgaaaaua acauuuacua caauagacau     2640

aacaucucca agcucgaguu cccccugccu uaugaaaacu uugauaaaga ggcuaaacuu     2700

aggguuuacg aacaggaccu ggauacguug aaauggaauc ucaagucaua uaacacauac     2760

acccagaaag acauagauaa ugugcacacu cugaucccaa gcauugccaa gaccaaaguc     2820

ucuggaaaga ugcacgagga gacauauuac gguaucaagg aaauuggcaa agacgaggau     2880

aagaaaaucu auaagacccu acgcacuccc auagaaaaag ugaagcgaaa ggaccuggac     2940

aacauuccgg aucggcucgg gggauccaaa gacgucuacg agagucuggu ggaaugguuc     3000

ggcgauuacg agguugguga ggaagugcuu aaggcaaaug ggaacaaaua uccuguaagc     3060

aagucugaca aagagcauaa ggaaaucaag aaauugaagc uguacuccga guauaaaaau     3120

acaggccaca ugaucaacaa gucaaauguc gagaaaggag gcauuuaccg uaucgaugug     3180

uuuaagagua aggacaaaga agaugacaag uuauauuucg ccgcuuacga uauacucgag     3240

auuaaaaaga ucaacacgcu gaaagaaaag aauagaguug acgauaacuu uaauauuaaa     3300

cuugacuaug ggcaaggaaa gaacagcaag aucaugucgu acaccgauau aauuaauaac     3360

uacgaccugu auaucucccu aaacaaaaau gaccucauca agauuacuac aaacgauggu     3420

aaagagucug uggcguacgu cgugggcugu agcucaggga ugcuggaagu aaagaguaaa     3480

uugggagacg gcuaugauau cauuaagacc gaccugaaug agaacaauau auucuccaag     3540

cagagggcac aguaccagau cacagugucu acuauuaaag agaucaagaa auuaagcauc     3600

uccguucucg gugaaauuuc agggcuguga                                      3630


<210>  2880
<211>  3633
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2880
augagaacca uccugggccu cgaccuuggg acaaacagca uuggaugggc cgugauaaau       60

uccgucauca aggagcagac ugaaaggauu uggaucgaga uggcugguuc ucggauuauc      120

cccauggaug cagccauacu gggcgacuuc gauaaaggga acucaaucag ucaaacggcg      180

gaacgcaccc gauuucgugg aguuagaagg uugcgggagc gccaguuacu gagaagggaa      240

cggcuacaca aggugcucaa aauucugggc uuccuuccug agcauuaccu gaaggguauc      300

gacuuugaga aacacacagg gaaguucuug agcggauccg aaccaaagcu cccgugggua      360

aaagaucgcu ggggcaagua uucuuuucug uuccagacug cuuuuaauga gaugcuggcc      420

gacuucgcaa aacaucagcc cucgcucgug uuugauggca agaaaauucc uuacgacugg      480

accgucuauu acuuacgaaa gaaggcccug acagaaauga ucucaaaaga ggagcuugcu      540

uggauauugc ugaacuucaa ucaaaagaga ggguauuacc agcuaagggg agaagaggaa      600

gaggauaacg cagguaaaag cguggaauuu uaugcccuca agguucugag uguggaggcu      660

accgacgaga aaaagggccg ggauauuugg uacaaugucc accuugaaaa cgggugggug      720

uaccgucgcu ccucuaaugu accacuggac ugggagggaa gaguuaaaga auuuaucgug      780

acuacagauu ugaaccccga cggcaccccu aagcucgaca aguaugguga ggucaaaagg      840

agcuuucggg ccccaaagga agaugacugg augcugauua aaaagcgaac ggaggcggau      900

aucacuggga cccauaaaac aaucggaucc uacauuuaug acgcauuacu gcaggagccc      960

cagcaaaaga uaaucggcaa gcucgugaaa acuguggaac gcaaguucua caaagaggaa     1020

cuuaauuuca uucugcagaa gcagugcgcc uuucacgcug aguugcagga uagagaccua     1080

uauaugaggu guaucgaagc ccuguacccu ggcaacgagg uccaacggcg caauauagca     1140

aacagagauu ucauuuaucu ccugaucgac gauguucuuu ucuaccagag gccacugaaa     1200

accaagaagu cauugaucgc uaauugcccg uacgaggaaa acgaguaugu gaaccguaaa     1260

acaggggaaa uuaagaccgc ccccuugaaa uguaucgcga agagucaccc ucuguuccag     1320

gaguuucggu uaugguacuu ccugagcaau auucgaguau aucaaaaaga gagagaagug     1380

aacggaacgc uacaucucga cgucgaugug acuacagagu uucugaagac cgaagacgac     1440

uacguugccc uuuucgauug gcucaauucu aggaaggaga uagaccagaa agcauuucug     1500

cgcuauccag cuuucggucu caagaaagaa aucaacaauu accgguggaa cuauguggag     1560

gauaaguuuu accccugcaa ugagacuaga cacgccauuc uguccaggcu ugaaaaagcu     1620

ggcaucggga ucgacuuccu gucagaugag aaggaaacag ccuuguggca uauucucuac     1680

agcgucagug acaaacagga gauagaaaag gcacuggcca ccuuugcugu gaagaacgga     1740

uuaaaugagu cuuucguaga gguuuucaaa aaguuuccuc cauucaaauc cgaauauggc     1800

gcguacagcg caaaggccau caaaaagcug cuaccccuca ugcgcugugg uaaguauugg     1860

gauaugucgc ugauugacgg gacgaccaaa gagcgaaucg aacggauuau cgcuggcgag     1920

ugcgaugaau cuauaggagc caaggugcgu gagaaagcaa ugaaccuuuc cgacaucuca     1980

uguuuuaaga gauugccgcu guggcucgcc ugcuacgucg uguauaauag gcacagugag     2040

gauaaagaaa uuacaaagug ggagacuccu gcugacaucg augccuaccu ggcagcguuc     2100

aagcagcaca gcuuacgcaa ccccauuguc gaacaaguga ucaccgagac acugcggguu     2160

gugagagaca uauggaaaca gguaggcaag auugacgaga ucaggauuga acuugggcga     2220

gagaugaaaa auccagcuga uaagcgcaaa cggaugacua cccagaucuu ggaaaacgag     2280

aaugccaacc ucagaauaaa ggcacugcua gccgaauuug ucaacccuga guauggagug     2340

gagaauguua ggcccuacuc cccaucucag caagaaaucc ugcguauuua cgaggacgcu     2400

gugcucaagg gugaggaaca gauccccgaa gauauugacg ucauccugaa aaaguucaac     2460

acaagcaaac uuccuaccaa gucagaguuu uugcgcuaua aacugugguu agagcagaag     2520

uaccggaguc cauauacugg cgaacucaua ccgcuggcca aacuuuucac ggcagcuuac     2580

gagauugaac augugauccc ccaguccaga uauuuugaug acagcuucuc uaauaaggua     2640

aucugugagu cagccgugaa caagcugaaa gauaaucaau ugggguacga auuuauuaag     2700

aaccaccaug gacagaaagu ugaggucggc uucgguaaga caguggagau cuuuagugug     2760

gacagcuaug aaagguucgu caaagagcag uacgcgaagu ccgggauuaa gaugaaaaag     2820

cuccugaugg augacauacc ugaacaguuc aucgagcgac aacuaaauga uucucgguac     2880

auuucgaaag uugugaaggg ccugcucagc aacaucgugc gcgagaaaaa ugacuccgga     2940

gaauaugagc cagaagccgu aucaaagaac auucuggucu gcaccggcag ugugacugau     3000

agacuuaaga aagacugggg gaugaaugac guuuggaacu cuaucgugag ccccagguuu     3060

gagcgguuga augcucugac cgaaacacag uguuucggac acugggagaa caaggauggu     3120

aaaaaggucu uucagaccgc agugccuuua gaguaccaaa aaggcuucuc caagaagcga     3180

auagaccaua gacaccacgc cauggaugcu aucguuauug ccugcgcaac ucgcaaucau     3240

gugaacuauc ucaacaauga aucagcgagc aggaacgcca aaaucucucg uuacgaccug     3300

cagcggcuuc ugugcgauaa guccagaggg gacggaaaug gcaacuauag guggauuauc     3360

aaaaagccau ggacaacguu uacccaggau gcucgcgagg cccuaagugg uauaauugua     3420

agccucaaac agaaucugcg aaucaucaac aagacuacaa auauuuacca acacuucgac     3480

gcaaacggga ccagagucua ugaaaagcag aaaauaggcg auucuugggc uauccggaag     3540

cccaugcaua aagacacagu guuuggagcc gugaauuuga ggaaggcacu gauuccgcag     3600

cacuacaacu uaacucucuu caucuccuca uga                                  3633


<210>  2881
<211>  3642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2881
auggagaaga gacugggcau cgacgugggg accaacagcc ucggaugggc cauugucgaa       60

guugaugacg aggguugcac acuucuggau aggggcgugg acauauucca ggaaggggua      120

gcucgggaga aaaaucaaga aaagcccgca gugcaggauc gcacugacgc ccgagcguug      180

cguagacacu acuuuaggcg gcgcuuaaga aaaaucgcuc ugcuaagggu ccucgugcgg      240

caugaucugu guccugcccu uuccgaggag cagcugaacg cauggcaggg aaagggcauu      300

uauccauugg acgaugccuu cauccgcugg caacgaacgg acgauaaugc ugacagaaac      360

ccguacaggg aucgguuucg uugccucacc gaagaacugg accuggcaug ucgcgaagag      420

agauauguuc ucgguagggc cuuauaccac cugugccagc ggcgaggguu ccuuucuaau      480

cgcaaagacg cuggaaacga ggccgaagag ggcaagguga agcaggaaau uaaaacauug      540

ucagcggaua uggcagccgc uggcugugag uaucuggggg aauacuuuua uagacuauac      600

cagaggaagg agaaaauccg gaagcauuac acugcccgca augagcacua ucucgcagag      660

uucagagcua uaugcaggaa acaacgucug gacgaggaac uucggcaggc ccuggagaag      720

gcgaucuuuu accagcgacc cuugaagagu cagaaaggac ucgucgguaa guguaccuuc      780

gaaaaagaua agagcagaug cccugugucc cauccaaggu uugaggaguu ccgcaugcug      840

caauuuauua acaauguacg gguuacaggc cccggggacg ccgaaccuag accauuaucu      900

cccgaggaag uggagaucgu caggccucug uuccuccgca aaucgaagcc acacuuugau      960

uucgaagaca uugcuaaaaa gaucgcagga aagggcaacu augccugucg aggugagcuu     1020

cugcaggcuc cguaccgguu caauugcgcc gggaaaucaa ccgugacugg aagccccgug     1080

agugcauugu uugccuccgu cuucggcgau gacuggcagc gugagauaug uucucuauau     1140

acacugggcg cugggaagaa cgaggagcaa auucucaaug auguuuggca cgugcuguuu     1200

gcguucgaag acgaggagag acuucaggca uggcugcagg aaaaauugca gcucagcgcc     1260

gaugacgcua agacccuggc cgcauuuagg uuaccucaag gauacgccuc ccugucacua     1320

aacguaauca auaaaauucu cccauaucug cgccgggguu acagauacga cgaggcugug     1380

uucuuugcca accuuggcaa ggucuugccc gcagaagugu augcggaucc ugcuaggcga     1440

cgcgagguug aacaggggau cagugcccag auggacgauc ugggagacgg cgagaagacg     1500

cggaaagagg aaagaaucag ggauuaccuc cgucgcuauu ggggugugga ggaaaagcgg     1560

cuggagagac uuuaccaucc aagcaugauu gaagcauauc ccucugccuc cccgaacggg     1620

cagggccugu ugcaacucgg aucaccuagg auacccgcug uccgaaaucc aauggccaug     1680

cgggcacugu uccgcuuaag agugcugcua aacgagcucc ugagggaggg caaaaucgac     1740

cgggggacua agauuaauau cgaacuugcu cgagaguuga acgaugccaa uaaaagaaag     1800

gcgauagaac gcuaccagag ggagcgugaa gccgagaacc ggaaguaugc ugaggaaauu     1860

agacagcagu acguagaggc aaccggacac agcaucgaac cuagugagga ggacauccug     1920

aaauacaggc ucuggguuga acaaggucau ugcucucugu auacaggcaa gacuauuggg     1980

aucuccgagu uuuuaggaga uggccgccga uacgacauug aacacaccgu gcccagaagc     2040

cggggugggg aggaugccca guauaauaaa acacugugcg aagaggaguu caacagggac     2100

auaaagcgcg auaaacuucc aucggaauug gcuaaucaca ccgccauccu cgcauguguc     2160

gagucuugcg gcugguuuga acguauugag ggacuggaac auagagugug gggcgcggac     2220

cggagggcca aguccgcuac gacuaaguca gccaaagacu ucgcaaucca ggaucgccac     2280

uacuggcgaa ugcaucuaga cuauuggaga cggaagugug aggcuuuuac aaugaccgag     2340

guccccgaag gguucaguaa caggcaggga guggauguug guaucauugg ccgcuacgcc     2400

agacuguauc uccaaacugu guucgacagg guauacacag ucaaaggggc aaccacggcc     2460

gcuuuucggc guaugugggg acugcaggag gaauacgcgg ccaaggagcg cgugaaucac     2520

guucaucacu gcauagaugc aaucaccauu gcuuguaucg gcagccgaga auaugcccuu     2580

ugggcccagu acgugagaca agacgaguug uauagguggg gugaggcuga uaaaccucgg     2640

ugcgggaagc cauggccgac auucacugaa gacguccgcg caaccgccga ugagcuguua     2700

auuucccacc auacacccga caacaugccu aaacagucua gaaagaggcu ccgggugcga     2760

ggcaagguac agagaaauga ucgcggagaa gugaaauaca ugcagggcga cacugcgcga     2820

ggggcucugc accaacagac cuuuuaugga gccaucgagc gggauggcga gauacguuac     2880

guugucagaa agagccuuga ccagcuguug ccagcagacg uggagaaaau cguggaugaa     2940

gccgucaggc agaagguucg cgaagcugug gaggcauaug guuucaaaga agccacagac     3000

cccaccaagc auacugugug gaugaacgag gagaagcaaa uuccuauccg gaaaguaagg     3060

auucucacgg gggucaagaa uccaauccug cuaaaacccc acagagaugu gucaguuaag     3120

gaauacaaaa gguacuauca cguggucaac gacggaaacu acuguauggc gauauaugag     3180

ggccgcgauc ggcaggguag acugaagcgu acauuugaaa uugugaauaa gcucgaggcu     3240

gccagguacu ucaaacggag ugcagaccgc gaaucccgac cggaucuggu uccucuuagc     3300

gacgugaacg gcuauucuuu gaaguaccug uuaaaaaccg ggacuauggu acucuuuuau     3360

gaaaauucac cagaugagcu guacgaaugc agugucggag agcuugccaa gagacuguac     3420

aaaguggugg gcauggcuca ggacgggagg guccaguucc uauuucauca agaagcccgg     3480

gaucagaagg caguuaccgc ugagugugga augggcauca gcguguucga cgcccgccac     3540

cccgcgccua agcucagaau uaggguaucc aacuuuaaaa uguucgugga agguuaugac     3600

uuugagcuga cagucaccgg ggaggugaag uucaaacgau ga                        3642


<210>  2882
<211>  3642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2882
auggagaaga gacugggcau cgacgugggg accaacagcc ucggaugggc cauugucgaa       60

guugaugacg aggguugcac acuucuggau aggggcgugg acauauucca ggaaggggua      120

gcucgggaga aaaaucaaga aaagcccgca gugcaggauc gcacugacgc ccgagcguug      180

cguagacacu acuuuaggcg gcgcuuaaga aaaaucgcuc ugcuaagggu ccucgugcgg      240

caugaucugu guccugcccu uuccgaggag cagcugaacg cauggcaggg aaagggcauu      300

uauccauugg acgaugccuu cauccgcugg caacgaacgg acgauaaugc ugacagaaac      360

ccguacaggg aucgguuucg uugccucacc gaagaacugg accuggcaug ucgcgaagag      420

agauauguuc ucgguagggc cuuauaccac cugugccagc ggcgaggguu ccuuucuaau      480

cgcaaagacg cuggaaacga ggccgaagag ggcaagguga agcaggaaau uaaaacauug      540

ucagcggaua uggcagccgc uggcugugag uaucuggggg aauacuuuua uagacuauac      600

cagaggaagg agaaaauccg gaagcauuac acugcccgca augagcacua ucucgcagag      660

uucagagcua uaugcaggaa acaacgucug gacgaggaac uucggcaggc ccuggagaag      720

gcgaucuuuu accagcgacc cuugaagagu cagaaaggac ucgucgguaa guguaccuuc      780

gaaaaagaua agagcagaug cccugugucc cauccaaggu uugaggaguu ccgcaugcug      840

caauuuauua acaauguacg gguuacaggc cccggggacg ccgaaccuag accauuaucu      900

cccgaggaag uggagaucgu caggccucug uuccuccgca aaucgaaacu ucacuuugau      960

uucgaagaca uugcaaagaa gaucgcugga aagggcaacu augccugucg aggugagcug     1020

uugcaggcuc cauaccgguu caauugcgcc gggaaaucaa ccgugacugg aagcccggug     1080

agugcacuau uugccuccgu cuucggcgau gacuggcagc gugagauaug uucucuguau     1140

acacucggcg cugggaagaa cgaggagcaa auucugaaug auguuuggca cgugcuuuuu     1200

gcguucgaag acgaggagag acugcagacc ugguugcagg aaaaacucca gcugagcgca     1260

gaugacgcca agacguuagc ugccuuuagg cugccccaag gauacgcauc ccuaucacuc     1320

aacguaauca auaaaauucu gccuuaucuu cgccgggguu acagauacga cgaggccgug     1380

uucuuugcua acuugggcaa gguccugcca gccgaagugu augcagaucc cgcgaggcga     1440

cgcgagguug aacaggggau cagugcucag auggacgauc ucggagacgg cgagcagacu     1500

cggaaggagg aaagaaucag ggauuaccug cgucgcuauu ggggugugga ggaaaaacgg     1560

cuugagagac uguaccaucc uagcaugauu gaagccuauc caucugcauc ccccaacggg     1620

caaggcuugc uccagcuggg aucaccgagg auaccugccg uccgaaaucc cauggcuaug     1680

cgggccuuau uccgccugag agugcuacuc aacgagcugc uuagggaggg caagaucgac     1740

cgggggacca aaauuaauau cgaauuggca cgagagcuga acgaugcuaa uaagagaaaa     1800

gccauagaac gcuaccagag ggagcgugaa gcggagaacc ggaaguaugc cgaggaaauu     1860

agacagcaau acguagaggc uacaggacac agcaucgaac caagugagga ggacauccuc     1920

aaguacaggc uguggguuga acagggucau ugcucuuuau auacuggcaa aaccauuggg     1980

aucuccgagu uucugggaga uggccgccga uacgacauug aacacacagu gccuagaagc     2040

cggggugggg aggaugcaca guauaauaag acccuuugcg aagaggaguu caacagggac     2100

auaaaacgcg auaaguugcc cucggaacuc gccaaucaca cggcuauccu ggccuguguc     2160

gagucuugcg gcugguuuga acguauugag ggacuagaac auagagugug gggcgcagac     2220

cggagggcga aauccgccac uacaaaguca gcuaaggacu ucgccaucca ggaucgccac     2280

uacuggcgaa ugcaucugga cuauuggaga cggaaaugug aggcauuuac caugacugag     2340

gucccagaag gguucaguaa caggcaagga guggauguug guaucauugg ccgcuacgcu     2400

agacucuauc ugcagacagu guucgacagg guauacaccg ucaagggggc cacgaccgca     2460

gccuuucggc guaugugggg acuucaggag gaauacgcug cgaaagagcg cgugaaucac     2520

guucaucacu gcauagaugc caucacaauu gcauguaucg gcagccgaga auaugcuuug     2580

ugggcccaau acgugagaca ggacgagcug uauaggcggg gugaggccga uaagccccgc     2640

ugcgggaaac cuuggccaac uuucaccgaa gacgucagag cuacagcaga ugaguuacuc     2700

auuucccacc auacuccgga caacaugccc aagcagucua ggaaacggcu gcgagugaga     2760

ggcaagguac agcgcaauga uaggggagaa gugaaguaca ugcaaggcga caccgccaga     2820

ggggcgcuuc accagcagac auuuuaugga gcuaucgagc gugauggcga gauaagauac     2880

guugucagga aaagccugga ccaguugcuc ccugccgacg uggaaaagau cguggaugag     2940

gcaguccgcc aaaaaguucg ggaagccgug gaggcuuaug guuucaagga agcaaccgac     3000

ccaacuaaac auacggugug gaugaacgag gagaagcaga uucccauccg aaagguacgg     3060

auucugacag gggucaaaaa uccuauccua cugaagccac acagggaugu gucaguuaaa     3120

gaauacaagc gcuacuauca cguggucaac gacggaaacu acuguauggc cauauaugag     3180

ggccgggaua gacagggucg ucucaaaagg accuuugaaa uugugaauaa gcuggaggcg     3240

gcucgguacu ucaagcgcag ugccgaccga gaauccagac ccgaucuugu uccguugagc     3300

gacgugaacg gcuauucucu gaaauacuua cucaagacug ggaccauggu acuguuuuau     3360

gaaaauucac cugaugagcu uuacgaaugc agugucggag agcuggcaaa aaggcuauac     3420

aagguggugg gcauggccca ggacgggcgg guccaauucc ucuuucauca ggaagcucgc     3480

gaucagaaag ccguuacagc agagugugga augggcauca gcguguucga cgcuagacac     3540

ccagccccca agcugaggau ucgaguaucc aacuuuaaga uguucgugga agguuaugac     3600

uuugaguuga ccgucacugg ggaggugaaa uucaagcggu ga                        3642


<210>  2883
<211>  3651
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2883
augaccaaga uccugggcau ugacacaggg acuaacagcc ucggaugggc cauaguggau       60

aaaacggcug acaauuacaa ccuucuggau aaggguguca auaucuucca ggagggcguu      120

aaaauugaaa aggggaucga guccucuaaa gcagccgaaa gaaccgagca cagggcgaca      180

cgggugcgcu auuaccgaau uaaguugcgu aagaucagau uacugaggau acuaucagac      240

aaccaucucu gcccaccucu gagucaagaa gaacuuagca cuuggcggcu gaaaaaggua      300

uaucccaaaa augaguuguu uaugcagugg cagcgcaccg augacgauac agaaaagacc      360

ccguacgcuu auagacacaa auguauccau gagaagcucg accuguccga ugugacugac      420

agguacauuc ugggacgggc cuucuaucac aucauucagc gccgaggcuu ucucucuaac      480

agaaaggaac aaggugggga ggauucggga aaagucaagg agggcaucuc aacauuaacc      540

caugaaauga augaggcagg cuacgaauac cugggggacu auuucuacgc ccuuuauaac      600

aaaggagaga agauaaggaa acacuacacg gcucggaaug aacacuuuuu gagcgaguuc      660

aaggcaauuu gcgagaaaca ggaucuggaa aaggagcuag guagugaagu gguucguaac      720

aucgagaagg ccauuuuuga ccagcgcccc cucaaauccc agaagggcca aguggggaaa      780

ugugucuucg aaaagaauaa aacuaagugc ccuucuagcc auccaaugua ugaggaguuu      840

agaaugcuga ccuucaucaa caauaucaag auuaaaacac ccaacgacga ugugcuuagg      900

ccucugaaua cuggagaacg ggagaagaua augccauugu uuuuccgaaa auccaagaaa      960

caguuugacu ucgaagauau caccaagaag cucgcuccca aaaagaugua cggcuucuau     1020

aaaaaggagg aagacgccga gaugccuuuu cuguucaacu acccaaugga uacaucagua     1080

agugguuguc cgguuaccgc ggcauuaaaa gacguguuug gggaugacug gauuacggcc     1140

aucugcgaga cuuacacacu gggagaaggc aagagccgcu ucgauauagu caacgacauu     1200

uggcacgcuc ucuauuuuua cucugauaag gacaaacuug ccgaguucgc aaucaauaga     1260

cugcaguugu ccgaagacga ggcuaagauc uuuucagaga uuagccuacc cgccgauuau     1320

gcgagucugu cucucaaagc caucuguaag auucugccuu accuuaggcg gggccugaua     1380

uauucccaug caguguucuu ggggaaccuc ugcgaaguga ugccaaaaua cgaguggagc     1440

aucaaggaaa ugcgcgagac cauuaucgac aauaucauua uggaaaugga uaaaugugac     1500

acuaaggaua agagaacauu ugaggucugc auaaaagacu aucugaaggg acaguacaac     1560

guuucggaua auucucugaa aaagcuguac caccccucca ugaucgagac cuauccuagg     1620

gugcaauaca cgaacgaaca cgguguauau cagcuaggcu caccacguau ugacagugug     1680

cggaauccca uggcuaugcg aagcauguuc agacucagga aacuggucaa ccgccuuuug     1740

guggagggga agaucgauca gaauaccgaa guucauauug aguuugcccg ggaacugaac     1800

gacgcuaaua agagaaacgc caucgcacuc uucgugaaag agaaucaauc cgccaacgau     1860

gaggcuagga agcagauaau cacacuguuu aaaaaggaaa cuggaaccga cauugagccg     1920

acagauaaag acauccuuaa guacguccug ugggaggagc agaaucacau uuguuuguau     1980

acuggcaagc agauccgcau aucugacuuc auuggugcga acccuaaauu cgauaucgaa     2040

cauaccauuc cccgaagcgu agggggcgac ucaacaaaga ugaaccucac ccugugcgau     2100

agucgguuua aucgugaggu gaaaaagacu aaauuaccag uugagcugcc uaaccacgac     2160

gaaaucaugg agagaauaaa ggaauggaag aaaaauuacg agcuacucga uauccaaauu     2220

aggaagaaca aaaagcuguc caaaggagca acgacaaagg agcagaaaga caccaucauu     2280

cgcaagcggc aucuuuugga acugagaagg gauuauuggc gaggcaagua cgagcgcuuc     2340

acuauggaaa gcgugcccga gggguuuucu cggagacagg gaaccgacau cucagucaua     2400

aguaaauacg ccaggcucua uuuaaagagc uuauucaauc guguguacac agucaaaggu     2460

auugcuaccu ccgauuuucg caagaucugg ggcauccagg acauuuauuc uaaaaaggaa     2520

cggaucaacc acgugcacca uugcauugau gccauaguua ucgcauguau ugggcuggac     2580

gaguacaaua aacuuggagc cuauuaccac gaugaggaaa acaauaaaug guauggcaug     2640

ucgaaggcua gcuucaaaaa gccaugggcc acuuuugugg aggacaucaa aaagauucaa     2700

gacgaaaucu ugguauacca uuacacaccc gauaacaugc cuaagcaggg uagaaggcga     2760

auaaaaaucg acgggaagaa aauucucugc aagggcgaug cagcgcgggg aucccugcac     2820

aaugacacgu auuacggcgc uaucgagaac gauggggccg ucaaauaugu gaagcgcauu     2880

gaccuagcau cacuggaaga gaaggauguu aaaaauaucg uggacgauac cgucagaagu     2940

auaauugagg ccgcuaucaa ggaaaaagga uucaaggacg ccauggcauc uacuaucugg     3000

augaacgagg aaaagagggu gccaauuaag aagguacggu guuuuacacc gagcauaacc     3060

aaaccccuca auauccgaaa gcagagagau guguccauua aagaguacaa gcagcaauau     3120

cacguugcua acgacucaaa cuaccugcuu gccuuguaua ucgguacaga caauaaaggc     3180

aaggaaaaac gcgaguucga gauugucaac auccugcagg cggcccagua cuacaggacu     3240

agcaaugaua aggaaguggu ggaccgucau auagucccua ucaagucuga gcacgauuau     3300

ccauuugcuu acaccuuaaa aauugggacg augguucucc uguaugaaaa gucccccaac     3360

gagguguggg acgcaaccau caaagagcgg aauagaaggc uuuacaaggu gacaggacug     3420

aguacuaugc gcaugaaagg ccgaaacggu gaauaugccu acgcuaccgu aaaguugauu     3480

cauaaugagg aagccagacc uagcaaggau aucaaagcaa agaacgggga guaugaacag     3540

ggcgaggagu uccggccagc gauaauuaug cuccacacac aacugaaugc ccuaguccag     3600

ggauacgacu uugaaaucaa cgagcugggc gaaaucaggc gccuccguug a              3651


<210>  2884
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2884
auggagaagc agagaauccu gggccucgac cccgggacca acagccuugg augggccgug       60

cuggauuucg acaauuccaa acaagaauac aacuugguca ggaaggguga gguuauuuuu      120

gaagagggcg uggggaaauc ugaacgggga gaguucucaa aggcugcaua ucgcacauuu      180

uacaaaagua cucgaaagca cuauuuccgu agaagguuac ggaagauaga gguacugaaa      240

gugcuaguca aguacgauuu uugcccuuau cucagcgacg aucugcuuaa acaguggcau      300

gaaaagaaaa ucuacccagc cgugaaggag uucauggacu ggcagcgcac guccgaugcg      360

gaaggcaaga auccguauua cgacagacac uguugccuga acgagaaauu ggaucucucu      420

cugaagucgg acagguacau ucugggucgg gcucucuauc auuuaaccca gcgccgaggg      480

uuuaaaucaa auagacugau ccaaaacaau gaugagaacg aagagggaaa gguuaaaaag      540

ggcauuagcg aacuuaguac agagaugcag gaagccaauu guaacuacuu gggcgacuau      600

uucuacaaac uguauacuga gaaggggaau accgugcaga ucaggaagcg guacacagau      660

cgugagaaac acuauaagaa cgaauuuaau gcaauaugcg agaaacagga acuaaacgag      720

accaucguca aggaacucga caaagccauu uucuuucaac gcaagcugaa gccccagaga      780

cauggagugg guaaauguac uuucgagccu aauaacaggc ggugcgcuau cucccaccca      840

gacuuugagc gauuccgcau gcuuuguuuc auuaacaaua ucaagauaaa aacacccuuu      900

gaagagaacc ugagaccuuu gaauaacgaa gagaaggcac ucauugaacc acuguucuac      960

aggaaaucua ucaagaauuu ugauuucgag gagauugcca agaaauuagc uggcaagaac     1020

aauuacugcu auaucaaaga cgauagcucc aagcaguaca aauuuaacua ucggauguca     1080

cagggggcca guaccuguca aacgaucgcg aagcucaaga gcauuuucgg agaagagugg     1140

aaaaauggca uagcagaaac uuacaccaac cgcagaaaga aagagggucu caagaaacag     1200

gccgagaugg uagacgauau cuggaauguu cuuuauucug uguccgaaug cgacguccug     1260

aaggauuuug cuauuaacaa auugcagcua gacgagaaga cagccgauaa uuucucaaag     1320

acuaaacugg cacaaggguu ugcuacccuc agccugaagg ccaucaggaa aauacuuccc     1380

uuccuggaac agggauuugg cuacacagag gcgguguucu uggccaacau uccuaccgug     1440

gucggcaagg aauauuggaa uaacgaggac auccguaacg auaucaugga ggaaauuaaa     1500

acgaucuuuu accacaauac ucucaagaca aacgacaguc auaauaccau ucuguucugu     1560

auaaaggaca ucuuaucuaa caauuucgau cuggcaccag gggcuauuga caaacuauac     1620

cacccgucca ugaucaacgu uuauaaggau gugaucccca augaggacgg uauucuccag     1680

cugggaagcc cuaaaauaga ugccguaaag aacccaaugg cuaugcgguc gcuucauauc     1740

auucgaaaag uggucaauga auugcugaga gagggcauca uuaacucuga cacugaagug     1800

aggaucgagu acgcccgcga gcucaaugau gcaaacaagc ggcacgccau agcugacugg     1860

cagaagaaac aauaugggaa gcaccagaaa uacagaaagg aaaucaaaga gcuguuuaag     1920

gcggaaacag gauucgagau ugaacccaau gagaccgaua uccuuaaguu ugagcugugg     1980

gaggaacaga accauauuug cuuguauacg ggccaggaaa ucgguauauc cgaguucguu     2040

ggggagaauc cuaaauacga cauugaacac accuuuccaa ggucacucgg cggagauagu     2100

acaaagauga acaugacucu gugcgagaac aaauucaauc gcgaaaacaa gcaaaagaaa     2160

uuacccagcg agcuggcaaa ucauuccgaa auccuagagc gaauugacga uuggaaaaag     2220

aagaucuaua uacuccagaa aaaguacgac ucuauccgga cccacaacgg cauggacaaa     2280

gagaauaagg auaaaaagau ugugagccgu cauaagauca aacuggaacu ugacuacugg     2340

aaggggaaau auaacagauu uacaaugacu gagauuccgg aaggauucuc aaggcgccag     2400

gguaugggca ucggguugau aagucgguac gccggacugu aucucaagac cauuuuucac     2460

aaaaaggaua auccucagaa guccaacguc uacaucguga aaggcagcau cacagcugag     2520

uucagaagga uuuggaauau ccaagacgau uaugaaaaga aaugucgaga caaccacauu     2580

caucacugca uagaugccau cguaguuggu uguauuggga agcgcgagua cgaccugaug     2640

gcacaguuuu aucgggauga ggaaaaauac agaugggaga agaagaaaaa gcccuucuuu     2700

aaaaagccau gggaaaccuu cacucaggac auguuaucuc ugaaagauga ggugcuuacg     2760

guccauuuca auccuucaaa cuuuacaaag aaggcccaca aaaagguguu cacccccaaa     2820

ggcaucuuug ucgcucaggg agacugcgcc agggugaagu ugcacaaagg caguuacuuc     2880

ggggcaauug aacaaaaggg agagaucaaa uauguuaugc guaaggagcu cagcgcgcug     2940

aagauagaag auaucaaaaa uauuguggac gcuguaguca aggagaaagu gcuagccguu     3000

gugaagaaaa aggguuuuaa gcaggcaaug gccgaaccaa ucuacaugaa cgaggacaaa     3060

cgcauucuga ucaagaaagu ccggcuguuc gugucccagg cuaauccccu gauaguaaag     3120

aaacauagag aucuuucuuc gaaggaguau aagcaaaacu acuaugugga caacgaaggc     3180

aauuugauga uugccaugua cgaggggguu aaaaagaacg gaaaaaucga uagggaaauc     3240

acugucguga auaaccugga ggcagcuaag uuuuuccgac agagccagaa aaauaacgcc     3300

gaaaagcagu uaauuuccca ccucucaccu aagaauggcu auccacugaa aaccgugcuu     3360

acacaaaagc agcuggucuu gauguacgag gacaguccga aagagauaaa gcuccgggau     3420

accaaaaaca ugguuaagcg ccuguaccag guguuuggua ucgaaaaaga cgggagagug     3480

aagcuaaagu uccaucagga ggcgaggucu gaaggccuga auauuaacag cgccgcuuuu     3540

aaaauccaag auacucccga gucccucuau cggcacacaa agucaaaucu gaaaguacuu     3600

gucaacggag uggacuucaa gauuaauauc uugggcgaaa uaagccugau cuga           3654


<210>  2885
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2885
auggagaagc agagaauccu gggccucgac cccgggacca acagccuugg augggccgug       60

cuggauuucg acaauuccaa acaagaauac aacuugguca ggaaggguga gguuauuuuu      120

gaagagggcg uggggaaauc ugaacgggga gaguucucaa aggcugcaua ucgcacauuu      180

uacaaaagua cucgaaagca cuauuuccgu agaagguuac ggaagauaga gguacugaaa      240

gugcuaguca aguacgauuu uugcccuuau cucagcgacg aucugcuuaa acaguggcau      300

gaaaagaaaa ucuacccagc cgugaaggag uucauggacu ggcagcgcac guccgaugcg      360

gaaggcaaga auccguauua cgacagacac uguugccuga acgagaaauu ggaucucucu      420

cugaagucgg acagguacgu ucugggucgg gcucucuauc auuuaaccca gcgccgaggg      480

uuuaaaucaa auagacugau ucaaaacaau gaugagaacg aagagggaaa ggugaaaaag      540

ggcaucagcg aacuuaguac agagaugcag gaagccaauu guaacuacuu gggcgacuau      600

uucuacaaac uguauacuga gaaggggaau accguccaga uuaggaagcg guacacagau      660

cgugagaaac acuauaagaa cgaauuuaau gcaaucugcg agaaacagga acuaaacgag      720

accauaguga aggaacucga caaagccauc uucuuucaac gcaagcugaa gccccagaga      780

cauggaguag guaaauguac uuucgagccu aauaacaggc ggugcgcuau uucccaccca      840

gacuuugagc gauuccgcau gcuuuguuuc aucaacaaua uuaagaucaa aacacccuuu      900

gaagagaacc ugagaccuuu gaauaacgaa gagaaggcac ucauagaacc acuguucuac      960

aggaaaucua uuaagaauuu ugauuucgag gagaucgcca agaaauuagc uggcaagaac     1020

aauuacugcu auauuaaaga cgauagcucc aagcaguaca aauuuaacua ucggauguca     1080

cagggggcca guaccuguca aacgaucgcg aagcugaaaa gcaucuucgg agaagagugg     1140

aaaaauggca uugcagaaac uuacaccaac cgcagaaaga aagagggucu caagaaucag     1200

gccgagaugg uugacgauau auggaacgug cuuuauucug ucuccgaaug cgacgugcug     1260

aaagauuuug cuaucaauaa guugcagcua gacgagaaaa cagccgauaa cuucucaaag     1320

acuaagcugg cacaaggguu ugcuacccuc agccugaaag ccauuaggaa gauccuuccc     1380

uuccuggaac agggauuugg cuacacagag gcgguguucu uggccaauau accuaccguc     1440

guuggcaaag aauauuggaa caacgaggac auucguaaug auaucaugga ggaaaucaag     1500

acgaucuuuu accacaacac ucucaaaaca aaugacaguc auaacaccau ucuguucugu     1560

auuaaggaca uauuaucuaa uaacuucgau cuggcaccag gggcuaucga caagcuauac     1620

cacccgucca ugauuaaugu guauaaagau guaaucccca acgaggacgg aauccuccag     1680

cuggguagcc cuaagauuga ugccgugaaa aauccaaugg cuaugcgguc gcuucauaua     1740

auccgaaagg ucgugaacga auugcugaga gagggcauua ucaauucuga cacugaaguu     1800

aggauugagu acgcccgcga gcucaacgau gcaaauaaac ggcacgccau cgcugacugg     1860

cagaagaagc aauaugggaa acaccagaag uacagaaaag aaauaaagga gcuguuuaaa     1920

gcggaaacag gauucgagau cgaacccaac gagaccgaua uucuuaaguu ugagcugugg     1980

gaagaacaga aucauaucug cuuguauacg ggccaggaaa uugguaucuc cgaguucgug     2040

ggggagaacc cuaaguacga cauagaacac accauuccaa ggucacucgg cggagauagu     2100

acaaaaauga acaugacucu gugcgagaau aaguuuaacc gcgaaaauaa acaaaagaaa     2160

uuacccagcg agcuggcaaa ccauuccgaa auccuagagc gaauugacga uuggaagaaa     2220

aagaucuaua uacuccagaa gaaauacgac ucuauccgga cccacaaugg cauggacaag     2280

gagaacaaag auaagaaaau ugucagccgu cauaagauca agcuggaacu ugacuacugg     2340

aaagggaagu auaauagauu cacaaugacu gagauuccgg aaggauuuuc aaggcgccag     2400

gguaugggca ucggguugau aagucgguac gccggacugu aucucaaaac cauuuuccac     2460

aagaaagaua acccucagaa guccaaugug uacaucguaa agggcagcau cacagcugag     2520

uuuagaagga uuuggaacau ccaagacgau uaugagaaaa agugucgaga caaucacauu     2580

caucacugca uagaugccau cguugugggu uguauuggga aacgcgagua cgaccugaug     2640

gcacaguucu aucgggauga ggaaaaguac agaugggaga aaaagaagaa acccuuuuuc     2700

aagaaaccau gggaaaccuu uacucaggac auguuaucuc ugaaggauga gguccuuacg     2760

gugcauuuca acccuucaaa uuucacaaag aaggcccaca agaaagucuu uacccccaag     2820

ggcaucuucg uggcucaggg agacugcgcc aggguuaaau ugcacaaggg caguuacuuu     2880

ggggcaauug aacaaaaagg agagaucaag uaugugaugc guaaaaagcu cagcgcgcug     2940

aagauagagg auaucgaaca uauuguagac gcugucguga aagagaaggu ucuagccgug     3000

gucaaaaaga aagguuucaa gcaggcaaug gccgaaccaa ucuacaugaa cgaggacaag     3060

cgcauucuga ucaaaaaggu gcggcucuuu guaucccaga cuaauccccu gauagugaaa     3120

aagcacagag aucuuucuuc gaaagaguau aagcaaaacu acuauguuga caacgaaggc     3180

aauuugauga uugcuaugua cgaggggguc aagaaaaacg gaaagaucga uagggaaauc     3240

accgugguga auaaccugga ggccgcaaaa uucuuucgac agagccagaa gaauaacgcu     3300

gaaaaacagu uaauuuccca ucucucaccu aagaauggcu auccacugaa gacaguccuu     3360

acccaaaaac agcugguuuu gauguacgag gacaguccga aggagauaaa acuccgggau     3420

acuaagaaca uggugaaacg ccuguaccag guguucggua ucgaaaagga cgggagagua     3480

gagcuaaaau uucaccagga agccaggucu gagggccuga auaagaacag cgcggccuuc     3540

aagauucaag auacacccga aucccucuau cggcauacga aaucaaaucu gaagguccuu     3600

gugaacggag uugacuuuaa aaucaauauu uugggcgaga ucagccugau auga           3654


<210>  2886
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2886
augaaguaca acuaugugcu gggccucgac aucgggauua gcuccgucgg auggggucuu       60

cuggccuugg augagaauaa caaucccuac aaaauaaucg acguuggcuc uagaauuuuc      120

ucaccugggg aaguggagaa gaccggagau aguaaagcua aggaaaggcg ggagaaacgc      180

ggcacacgac guguaaucag aaggcgggaa uuucgcuuag acagagugag gaaccugcua      240

uaugagaagg guuaccucaa ggggaauauu acuagcaaca ucgucuccgu gaaaaaugag      300

gaacugacga uaaucuucaa cgauaugguu aauaacuauu acaagucuaa agacaccaau      360

ccauauaagc ugaaagugga ggcacuggau cggaaguuga caaacgacga acucucgauu      420

auccuggucc acuacgccaa gaaacgcgga uacaagucaa aucgagagga gagcagugau      480

aacgacaaug gcaaagugaa ggaagcgauu aaagagaaca cuaaccugau ggaagagaag      540

aaauauagaa ccaucuccga aauguacaua aagaaugaga aguuuaaaaa caagauuaaa      600

aauggcccgg augacuauaa gcucucugua acaaacgaga uguacuuaga agagaucaau      660

aaaguucugg auagccagau uggguucgga cuuaucgacg acaacuuuaa gaaugaauau      720

uuggagaucu acaacuccag gcggcauuau ucagaagguc ccggcuacua cuaugcuaag      780

gaugccaaug ggaacaaagu gaagguccgu aguccuuacg gaggcgaccu gauuaaaaag      840

augaccggug ugugcagcuu cgauaaaaag gcacgcgccc caaaguaugc uuuuucuucc      900

gagcuauucg ugucaauaag caaacucguc aaucugagau acaagaucga cgaugacagu      960

uauaaacaac uuacuaagga gcagauuaac gaacugaucg augaggcuaa aaauaagaac     1020

aagguuacau acaaaucuuu gauaaagauu cucgggauca aaaauaucaa ggugaaagac     1080

cugaacuuau ccaagagcga auauauuaaa guaaucgaug agcugaagaa gaaacucaac     1140

auuucgaagg aagagaaaau agacaucuac ucucuugaug aggaaaauaa gaaaauuuac     1200

gacgacaucu auaacgagaa gcuguuuucc aagaccuuga ucgaguucaa aggauacaau     1260

cuacugaaga aagccauuac gcucucauau aacaguggca cuuggaauga ugugaaggac     1320

aacauagaau uucuggauga gcuugcucug uacuguaccg acuauaaagu caauaacgaa     1380

auccaggagc acauucauaa uagcuugauc auugauucca gguucgacga uucuaacuuu     1440

guggaaaauc uccccaacuu caaggaccac cugauguuaa gcacagauau cauacggaag     1500

cugaucccua uuaugcuaga gggcaucacu uacgacaaag ccauggauaa gauuggguau     1560

aaucauucaa accucuacaa agacaccauc aaggaagauc ugcuuguucc aguguacguc     1620

aguaaaaaua uaacaaacca gcgagugauu cgcuccuuga cccaagcgag aaagguaauc     1680

aacgcaauua ucaagaaaua uggaagcccc aagauaauca auauugagac ggccagggag     1740

cuggcuaaaa cucagaagga acggcgcauc auugagaaaa accagcagga caagcaauac     1800

gaaaaugaga gaaucaaaaa gcaccucguu gaacuggagc uuuucucuga cgagucaaag     1860

auaacaggua acgaucuguu gaaauauaag cucuggcagg aacagaaaga guuuugcggc     1920

uacaguauga agaaaauuaa gcuggaagac uuauucagca auaacaucgu gcagaucgau     1980

cacauucugc cuuauuccag gaccuacaau gacaacuauc uaaauaagac ucucgucuac     2040

acaaaauaca accaagauaa ggggaauaaa accccauaug agugguuugg aaagacggac     2100

aaauggcagg aauacgaggc cuucaucaac ucucugucga uuagccagaa uaagaaggau     2160

aacuaucuuu ugaaaaaucu ggaccaagau gcagagcgug aaaugcggga gcagaaccuc     2220

aacgacacca aguacauauc ccgagaacug uuaucacuga ucaaagcaua ucuuaaugug     2280

gaugagguca acgccuuucc gggcgcgauu acagccaagu ugagagcaag gugggguuuc     2340

aaucgccuca cucauaguua caucucuaaa accuauauua ugcccgagga caugaagaag     2400

gggaucaaca aagaucggga caaucaccuu caucacgcua uggacgcccu agugauagcu     2460

agcaucacaa agucccugca gcagaaaauu acucucuacg aaaaguuuuc aagauacguu     2520

gauggcauca ccaaaaacga gcugggaaag auuagcacag gcaauaucaa cgacgaucuu     2580

uauaauuacu augacgaaga gaccggggaa auaaaggagu uggagcugag gcaauacauu     2640

aaaaaccagg uggcccagaa uaacaucuau uucucuaagc acaauguauc caaauuaauc     2700

uuuccuacuc cauacaacaa uuucgucgaa gaggcaaagc uccgcgugua ugaacgagau     2760

cuggagguuc uuaaauggaa ccugaagaau uugggaacgu acacaaguag cgaacucaac     2820

cggauucaug ugcugauacc cucugucgcc aaaaacaaga ucuccggucg ucuacacgag     2880

gagaccuacu auggccugaa ggaagugggg auuggagagg acaaaguaac uuacaagacc     2940

cucaaaacac cuaucucaaa ggugaaaaag aaggaucugg acaauauucc agauuguaaa     3000

ggcgguagua aggacaucua uaacgcucuu guuaaauggu uugggaauug cgaaaccggc     3060

gaggaagcgu ugaagaugca ugauggaaaa uaccccguca acccgaauga caaggagcag     3120

aaggagauaa gaaaaaucaa gguguauacu aaauacaacg caacaggcca cguggucaau     3180

aacgggaaug uugaaaaggg agguauuuau aggaucgacg uguucaaaag caagaacaag     3240

gaggauaaua aacuguacuu uacgucguac gacauuuucg aaaucgagca aauaaaggcc     3300

uaugagaaag gcaagaacau uaauuucucc aucaaauuag aauacgggca gggaaagaac     3360

aauaaaaucc uccuguauaa ggaugugaag aacgaguacg accuuauuau gucuaucuau     3420

aaaaacgauc ugauacgcau uaagaccaaa acuggcaaua agagcacagc uuacguaguc     3480

gguuuuucag ggggcaaacu agaaaucaag aguaccauug gagacggcua ugauaucaua     3540

gggaacaaua acauuuucga caaguccaug gagcgguaca gagugacauc uagcacuauc     3600

guuaaaaucg auaagcucuc cauuucaauc cugggagaag ugagcggcuu guga           3654


<210>  2887
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2887
augaacaaga gaauccuggg ccucgacacc gggacaaaua gccuuggaug ggccgugguc       60

gauugggacg agcacgcuca guccuacgaa cugauuaaau auggugaugu uauauuccaa      120

gagggcguga agaucgaaaa agggauugag ucuucaaagg cagccgaaag gaguggauac      180

aaagcgaucc ggaagcagua uuuucgccga cguuugagaa agauucaggu auuaaaagug      240

cuggucaagu accaucuaug ccccuaucuc agcgacgaug accugaggca guggcaccuu      300

caaaaacagu acccuaaguc cgaugagcug auguuguggc agcggacuuc ugacgaggaa      360

ggcaaaaacc cauauuacga ucgccauaga ugucuccacg agaagcugga ccugacggug      420

gaagcugaua gguacacccu cggucgggcc uuauaucauc ugacacagcg ccgaggguuc      480

cuuucgaaua gauuggacac uucagcagau aacaaggagg acggaguugu gaaaagcggc      540

aucagucaac uguccaccga gauggaagag gccggcugcg aauaccuagg ggacuauuuu      600

uacaagcucu aucacgagca gggaaauaaa gucaggauac ggcagcguua cacagaucgc      660

aagaaacacu aucagcauga guucgacgcu aucugugaaa agcaagagcu gucuagcgaa      720

cuuauugagg aucugcagag agcaaucuuu uuccaguugc cgcucaaauc ccaaaggcac      780

ggugugggcc ggugcaccuu ugaacgaggg aagccccgcu gugccgacuc acauccugau      840

uacgaggagu ucagaaugcu gugcuuugua aacaauauuc agguuaaggg accacacgac      900

uuagaacuga ggccccucac uuacgaggaa cgggagaaaa ucgaacaccu uuucuuucgc      960

aagaguaaac cuaacuucga uuuugaggac auagcuaagg aacuggccgg caaaaagaau     1020

uaugcgugga uucaugauaa ggaggagaga gcauacaaau ucaacuauag gaugacacag     1080

ggugugccag ggugucccac cacggcccag cuaaagagca ucuucggaga cgauuggaaa     1140

acuggcauug cugaaaccua cacauugauc caaaagaaaa auggcucuaa gucccugcag     1200

gagauggucg acgacgugug gaacgugcuc uauucauuua gcagugucga aaagcugaaa     1260

gaguucgccc accauaaacu ucagcuggau gaggaaaaug cagagaaauu ugcuaagauc     1320

aaauugucuc acuccuucgc cgcgcucagc cugaaggcca uucguaaguu uuuaccuuuc     1380

cugcggaaag ggauguacua uacucaugca ucguuuuucg cuaacauacc aaccaucguu     1440

ggaaaggaaa uuuggaauaa cgagcagucu cgaaaauaca ucauggaaaa cgugggugag     1500

cuaguauuua auuaccaacc gaagcacaga gacgugcagg gcacaauaga gaugcucauu     1560

aaagauuucc uggccaacaa uuuugaacuu cccgcugggg ccaccgacaa guuguaucac     1620

ccuuccauga ucgagacgua cccacaugca cugaggaacg aguucggaau ccuccagcug     1680

ggcucacccc gcacuaaugc cauucggaac ccuauggcua ugagaagucu ucacguccug     1740

aggcgcgugg uuaaucaguu gcucaaagaa agcaucauug augaaaacac agaggugcau     1800

gucgaguaug cgcgagaacu gaaugacgca aacaagcggc gugccauagc ugauagacaa     1860

aaggagcagg acaaacagca caagaaauac ggugaugaaa ucaggaaguu auauaaagag     1920

gaaaccggga aggacauuga gccaacucag acagaugugc ugaaguuuca acuaugggag     1980

gaacagaauc acuacugccu cuauaccggc gagcagaucg gaaucacgga cuucauuggc     2040

uccaacccca aauucgauau agaacauacc aucccgcaau cuguaggggg agacagcaca     2100

cagaugaauc ugacucuuug ugacaaccgc uuuaaucggg agguuaagaa agccaaguug     2160

ccuaccgagc uggcaaacca cgaagagauu cucacaagaa ucgaacccug gaaaaacaag     2220

uacgagcagc uggugaagga aagggauaaa cagcgaacuu ucgccgguau ggacaaggcu     2280

gucaaagaua uucgcaucca aaagcggcau aaauuacaga uggagauaga cuacuggaga     2340

ggcaaguaug agagguuuac caugacagaa gugccagagg gguucucacg ucgccaggga     2400

accggcaucg gucugauuag ucgguacgcc gggcuuuauu ugaagucccu cuuucaccag     2460

gcagauagca gaaauaaauc uaacgucuac gugguuaagg gcguggcgac ugcugaguuc     2520

aggaaaaugu ggggacugca aucagaauau gaaaagaaau gccgagacaa ucauagucac     2580

cacuguaugg augccaucac gauugcaugc aucggcaagc gggaguacga ccuaauggcc     2640

gaguauuacc auauggaaga gacauuucgc caggggagag gaagcaaacc uaaguucucc     2700

aagcccuggg cuaccuuuac ugaagaugua cugaacauau acaaaaaucu ccuggucgug     2760

cacgacaccc caaacaauau gcccaagcau acaaagaagu auguucagac cucuaaaggu     2820

aaggugcuug cccagggcga uacugcaagg gggucguugc accuggacac auacuaugga     2880

gcuauugagc gggauggcga aauccgauac gucgugagac gcccuuuaag cuccuucacg     2940

aacccaaagg agcucgagaa uauuguagac gaaaccguga aaaggacuau caaggaggcc     3000

auagcggaca aaaacuuuaa gcaagccauc gcugaaccga uuuauaugaa ugaggauaaa     3060

gguauccuga uuaagaaggu ucguugcuuc gcaaacucag ucaaacagcc caucaauaua     3120

cggcagcaua gagaccuuag uaagaaagag uacaagcaac aguaucacgu gaugaacgaa     3180

aauaacuacc uguuggccau uuacgagggg cucgugaaaa acaaggucgu uagggaauuu     3240

gagaucgugu cuuauaucga agcugccaag uacuauaaac gcagccagga ucgaaauauu     3300

uucuccucaa ucgugccuac acacagcacc aaguacggcc ugccacuaaa aacaaagcug     3360

cucaugggac agcaaguacu gauguucgag gagaaccccg acgaaauuca ggucgauaau     3420

acuaaagacc uugugaagag auuguauaag guugugggca uagagaaaga ugggcggauc     3480

aaguuuaaac aucaccagga agcaaggaag gagggacuga ccauuuacuc uacgccuuau     3540

aaaaacaaug acgauuacgu cccaaucuuc cgccagucca uuaagaacau caauauauua     3600

guggacggca ucgauuuuaa cauugacauc cucgguaaag uuacccugaa ggaauga        3657


<210>  2888
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2888
augaacaaga gaauccuggg ccucgacacc gggacaaaua gccuuggaug ggccgugguc       60

gauugggacg agcacgcuca guccuacgaa cugauuaaau auggugaugu uauauuccaa      120

gagggcguga agaucgaaaa agggauugag ucuucaaagg cagccgaaag gaguggauac      180

aaagcgaucc ggaagcagua uuuucgccga cguuugagaa agauucaggu auuaaaagug      240

cuggucaagu accaucuaug ccccuaucuc agcgacgaug accugaggca guggcaccuu      300

caaaaacagu acccuaaguc cgaugagcug auguuguggc agcggacuuc ugacgaggaa      360

ggcaaaaacc cauauuacga ucgccauaga ugucuccacg agaagcugga ccugacggug      420

gaagcugaua gguacacccu cggucgggcc uuauaucauc ugacacagcg ccgaggguuc      480

cuuucgaaua gauuggacac uucagcagau aacaaggagg acggaguugu gaaaagcggc      540

aucagucaac uguccaccga gauggaagag gccggcugcg aauaccuagg ggacuauuuu      600

uacaagcucu augaugcuca gggaaauaaa gucaggauac ggcagcguua cacagaccgc      660

aacaagcacu aucagcacga guucgaugca aucugugaaa aacaagagcu gucuagcgag      720

cuuauugaag accugcagag agccaucuuu uuccaguugc cgcucaaguc ccaaaggcau      780

ggugugggcc ggugcaccuu ugagcgaggg aaaccccgcu gugcugauuc acacccugac      840

uacgaagagu ucagaaugcu gugcuuugua aauaacauuc agguuaaggg accacaugau      900

uuagaacuga ggccccucac uuacgaggag cgggaaaaga ucgagccucu uuucuuucgc      960

aaaaguaagc caaauuucga cuuugaagau auagccaaag cgcuggcagg caagaaaaac     1020

uaugccugga uucacgacaa ggaggaaaga gcuuacaagu ucaauuauag gaugacacag     1080

ggugugcccg gguguccuac caucgcccag uugaaaagca uuuucggaga ugacuggaag     1140

acgggcaucg cagagacuua cacccuaauc caaaagaaga acggcucuaa aucccugcag     1200

gagauggucg acgaugugug gaaugugcuc uauucauuua gcagugucga aaagcugaag     1260

gaguucgcuc accauaaacu ucagcuggac gaagagucug ccgagaaguu ugcgaaaauu     1320

aaguuguccc acagcuucgc cgcacucucg cugaaagcua uacguaaguu uuuaccauuc     1380

cugcggaagg ggauguacua uacacaugcc uccuuuuucg cuaacauccc gacuauuguu     1440

ggaaaagaaa ucuggaauaa ggagcagaac cgaaaauaca uaauggaaaa cgugggugag     1500

cuaguauuua auuaccaacc caagcacaga gaagugcagg gcaccauuga gaugcucauc     1560

aaagauuucc uggccaacaa uuuugagcuu ccugcagggg ccacagacaa guuguaucau     1620

ccaucuauga ucgaaaccua ccccaacgcu cagaggaaug aguucgggau ucugcagcuc     1680

ggcucaccuc gcacgaacgc gauccggaau ccaauggcaa ugagaagucu gcacauucuu     1740

aggcgcgucg ugaaccaacu guugaaagaa agcauaaucg augagaauac ugaaguucac     1800

guggaguaug cccgagagcu caacgacgcu aauaagcggc gugccauugc agauagacag     1860

aaggaacagg acaaacagca uaagaaauac ggugaugaga ucaggaagcu guauaaagaa     1920

gagacaggga aggacaucga acccacccaa acugaugucu uaaaguuuca gcugugggag     1980

gagcagaacc accauugccu auacacaggc gaacaaauug gaauaaccga cuucaucggc     2040

uccaauccga aauucgauau ugagcacacg aucccucagu cugugggagg agacagcacc     2100

cagaugaacc ucacacugug ugacaaucgc uuuaaccggg aaguaaagaa agccaaacuu     2160

cccacugagu uggcuaacca cgaggaaauu cugaccagaa ucgagccaug gaaaaauaag     2220

uaugaacagc ucguuaagga gagggauaaa caacgaacau ucgccgguau ggacaaggca     2280

gugaaagaua uacgcaucca gaagcggcau aaacugcaga uggaaauuga cuacuggaga     2340

ggcaaguacg agagguuuac uaugaccgag gucccugaag gguucucacg ucgccaggga     2400

acaggcaucg guuuaauuag ucgguaugcg gggcuguacc uuaaguccuu guuucaccaa     2460

gcugauagca gaaacaaauc uaauguguau gucgugaagg gcguugccac cgcagaguuc     2520

aggaaaaugu ggggacucca gucagaauac gagaagaaau gccgagacaa ccauagucac     2580

cauuguaugg augccaucac uauagcuugc auuggcaagc gggaauauga ccugauggcc     2640

gaguacuauc gcauggagga aacguuuaaa caggggagag gaagcaagcc caaguucucc     2700

aaaccauggg caacauuuac cgaggaugug cuaaauaucu acaagaaccu gcucguaguc     2760

cacgacacuc ccaauaacau gccuaaacac accaagaaau acgugcagac aucuauuggu     2820

aagguucugg cucaaggcga uaccgccagg gggucgcuuc auuuggacac uuauuacgga     2880

gcgaucgaac gggauggcga gauacgauau guggucagac gcccacugag cuccuucaca     2940

aagccggaag aguuagagaa uaucguggac gaaacgguaa aaaggaccau uaaggaggcc     3000

aucgcugaca aaaacuuuaa gcaggcaauu gccgaaccca ucuacaugaa ugaggagaaa     3060

gguauacuca uuaagaaggu gcguugcuuc gcuaaaucag uuaagcagcc uaucaacauc     3120

cggcaacaca gagaucugag uaaaaaggaa uauaaacagc aguaccaugu caugaaugag     3180

aacaauuauc uucuggccau uuacgaaggg uuggugaaga acaagguggu cagggaguuu     3240

gaaaucguuu cuuacauuga ggcagcgaaa uauuacaagc gcagccagga ccgaaacaua     3300

uucuccucaa ucgugccaac ucacagcaca aaauauggcc ucccccugaa gaccaaacua     3360

cugaugggac aacucgugcu gauguucgag gaaaauccug augagauuca gguagacaac     3420

acaaaggauc uugucaagag auuguacaaa gugguuggca ucgaaaagga cgggcggauu     3480

aaauuuaagu aucaucagga ggccaggaaa gaaggacugc caaucuucuc uacucccuac     3540

aagaauaacg augacuaugc uccgauauuu cgccagucca ucaauaacau uaauaucuua     3600

guggauggca uugacuucac caucgauaua cucgguaaag ucacgcugaa ggaguga        3657


<210>  2889
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2889
augaacaaga gaauccuggg ccucgacacc gggacaaaua gccuuggaug ggccgugguc       60

gauugggacg agcacgcuca guccuacgag uucauuaaau auggugaugu uauauuucaa      120

gagggcguga agaucgaaaa agggauugag ucuucaaagg cagccgaaag gaguggauac      180

aaagcgaucc ggaagcagua uuuccgccga cgucugagaa agauucaggu auugaaagug      240

uuagucaagu accaucugug ccccuaucua agcgacgaug accucaggca guggcaucug      300

caaaaacagu acccuaaguc cgaugagcuu augcuguggc agcggacuuc ugacaaaaag      360

ggcaagaacc cauauuacga ucgccacaga uguuugcacg agaaacucga ccugacggug      420

gaagcugaua gguacacccu gggucgggcc cucuaucauu uaacacagcg ccgaggguuu      480

cugucgaaua gacuugacac uucagcagau aacaaggagg acggaguugu gaaaagcggc      540

aucagucaau uguccaccga aauggaggag gccggcugcg aauaccuggg ggacuauuuc      600

uacaagcuau augaugagca gggaaauaaa gucaggauac ggcagcguua cacagaccgc      660

aacaagcacu aucagcacga auuugaugcu aucugugaga aacaagaacu cucuagcgag      720

cugauugagg accuucagag agcaaucuuc uuucagcugc cguugaaguc ccaaaggcau      780

ggugugggcc ggugcaccuu cgaacgaggg aagccccgcu gugccgauuc acacccugac      840

uacgaggaau uuagaaugcu cugcuucgua aauaacauuc agguuaaagg accacaugau      900

cuggaguuaa ggccccugac uuacgaagaa cgggagaaga ucgaaccucu cuuuuuccgc      960

aaaaguaagc caaauuuuga cuucgaggau auagcuaaag cccuugcggg caagaagaac     1020

uaugcaugga uucacgacaa agaagagaga gccuacaagu ucaauuauag gaugacacag     1080

ggugugcccg gguguccuac cacggcucag cugaaaagca ucuuuggaga ugacuggaag     1140

acuggcauug ccgaaaccua cacauugauc caaaagaaga acggcucuaa gucccuacag     1200

gagauggagg acgaugucug gaaugugcug uauucauuca gcagugugga aaaacucaag     1260

gaguuugcac accauaaacu gcagcuugac gaagagucug cugagaaguu cgccaaaauc     1320

aagcuguccc acagcuuugc ggccuugucg cucaaggcaa uucguaaauu ccugccauuu     1380

uuacggaagg ggauguacua uacucaugcu ucuuucuuug ccaacauacc gaccaucguc     1440

ggaaaagaaa uuuggaauaa cgagcagaac cgaaaguaca ucauggaaaa uguuggugag     1500

cugguguuca acuaccaacc caaacacaga gaaguacagg gcacaauaga gaugcuaauu     1560

aaggauuuuc ucgcuaauaa cuucgagcug ccugccgggg caaccgacaa acuuuaucau     1620

ccauccauga ucgaaacgua cccccacgcc cagaggaaug aguuuggaau cuugcagcug     1680

ggcucaccuc gcacuaacgc uauucggaau ccaauggcga ugagaagucu ccacauccug     1740

aggcgcgugg ucaaccaacu ucugaaggaa agcauuauag augagaauac agaagugcau     1800

guugaguaug cacgagaguu gaacgacgcc aauaagcggc gugcuaucgc cgguuggcag     1860

aaagaacagg auaagcagca caaaaaguac guggacgaga uuagaaaacu cuauaaggaa     1920

gagaccggga aggauaucga acccacucaa acagacgucc ugaaauucca guuaugggag     1980

gagcaggauc aucacugccu guacaccggc gaacaaaucg gaauuacgga cuucauaggc     2040

uccaacccga aguuugauau cgagcacacc auuccucagu cuguaggggg agacagcaca     2100

cagaugaauc uaacucucug ugacaacagg uucaaucgcg aaaucaaaaa ggcaaaacug     2160

cccgcccagc uuucaaacca ugaggagauu uuggcucgga ucgaaccaug gaagaacaag     2220

uaugagcaga aaauaaagga aagagauaaa caaaggaccu uugccgguau ggacaaggca     2280

gugaaagauc ugcgaaucca gaagcgccac aagcuccaga uggacauuga uuacuggcgg     2340

ggcaaauacg agagauucac aaugacugaa guuccugagg gguuuaguag gcgucaagga     2400

accggcaucg gucugauuuc ccgcuaugcg ggguuauacc ugaaaagccu uuuccaucag     2460

gcugacucuc ggaauaaauc aaacguguau gucgugaagg gcgucgccac agcagaguuu     2520

agaaaaaugu ggggauugca gagugaauac gagaagaaau gcagggauaa ucacagccau     2580

cacuguaugg acgccaucac cauagcuugc auuggcaagc gagaauauga ucucauggcc     2640

gaguacuauc ggauggaaga gacuuuccgc caggggagag gauccaagcc caaauuuucu     2700

aagccauggg caacguucac agaggacgug cugaacaucu acaaaaaucu acugguugug     2760

cacgauaccc ccaacaauau gccuaagcau acuaaaaagu acguacaaac cucgaagggu     2820

aaaguccucg cucagggcga cacagccagg gggagccugc accuugauac cuauuacgga     2880

gcgauugaac gggacggcga gauccgauau gugguuagac gcccauuguc cucauuuacu     2940

aagccgaaag aacuggagaa cauaguggac gaaacaguca agaggacgau caaagaggcc     3000

auugcugaua agaauuucaa gcaggcaauc gccgagccca uuuacaugaa cgaagacaaa     3060

gguaucuuaa uaaagaaagu gcguugcuuu gcuaagagug uaaaacaacc uauuaauauc     3120

cggcagcaua gagaucucuc uaagaaggag uauaaacagc aguaccacgu gguuaacgaa     3180

aauaacuaua ugcuggucau cuacgagggg cuugugaaga auaaaguggu cagggaguuc     3240

gaaauuguua gcuacaucga ggccgcaaag uauuacaaac gcucccaaga ccgaaacauu     3300

uucucaagca uagugccaac ccauucugcg aaguauggcc ugcccuugaa gacuaaacuc     3360

cugaugggac agcuagugcu gauguuugaa gagaacccug augaaaucca gguagacaau     3420

acaaaggauc ucgucaaaag acuguacaag gugguuggca uuaaaaagga cgggcgggug     3480

aaauucaagu uucaccagga ggccaggaag gauggacuuu ugaucuucuc caccccauau     3540

aaaaacaaua acggcuacgu ccccauuuuu cgccacagua agaaaaauau caacauacug     3600

guggacggua aggauuucac aaucgacauu uuagggaaag uuacucucaa ggaguga        3657


<210>  2890
<211>  3660
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2890
augaaggagg aagugagagg caaaaacagg agccagaccu gggccauccu ggcuaaguuc       60

uuugucuggu ccgagaaaga aucuguuugg aaugagaagg aagcacggac agaggagcgc      120

gugaaagaau acaagcucgu agggcuuaag cgagacacua aaggaaacga gcugaaguug      180

gaaaauuauc guuggagagu gcaagccuua ucagagcagc ugaaccccga gauugucgcg      240

auagugcuac aggaaaucaa uggucagauu agugcuagcu ccggcuaccu cggggccauc      300

ucugauaggu cgaaacaacu guauuucaac cggcagacgg uuggacagua ccuuauguca      360

gagcuggaaa aggacccuaa ugugagcuug cgcaacagag ucuuuuauag gcaggauuac      420

cucgacgagu ucgaucggau uugggaaaaa caagcagagu uucacgagga acugaccgug      480

gagcugaaga aggaaauccg cgacauaauc auuuucuauc agcgaagacu caaaagucag      540

aagggcuuaa ucuccuuuug cgaguucgaa aagagggagg uaguugugga ggaagauggu      600

cggaagaaaa auaagacagu cgggugucgu gugauuccac gcucucaucc gcuguuucag      660

gaguucaaaa ucuggcaaac ucuuaacgac auagaagugu uugccuggga uaagcagagc      720

aagcgcaaaa aggcugacaa auccucaauu uuguucgaua auagugagga cgcacugcua      780

gucgaaggaa agagguuucu cuaccaggag gagaaagaac ugcuugccaa ggagcuguuc      840

guuaaggaaa gcaugaaaaa ggcugaggug uugaaacucc uguucgagaa cuaccaagaa      900

uuagaucuga auuuuaagca gaucgacggc aaccacaccg gcuucgcccu cuuuucugcg      960

uauuccaaaa ugauugagaa guacggguau gaacccguag acuucaagcg gucagcagau     1020

gagaucaucg aaaaacuuga gacaauuuuu accaaucugg gauggaacac ugaguugcua     1080

agcauagacc ugaguaagga agauaaagag cucgaacgac agucuuacuu ccgccugugg     1140

caucuucugu auuccuuuga gggugacaac acaccuaccg gcaaugggaa guugcucgag     1200

aaaaucaugc agcugugcga uguggaaaag gaguacgccg ucgaauuagc uaacgugagc     1260

uuccaagagg acuauggauc gcugucugcc aaggcaauua aaaagauccu accauaccuc     1320

aaagaaggca aucaguacga uguugcuugu gacuaugccg guuacagaca cuccagguca     1380

agucugacga aggaugagau agaaaacaaa auucuuaagg acaaauugga gauccugccc     1440

aagaauagcc uccggaaccc uguggucgag aagauccuga aucagauggu gaaccuuauu     1500

aauacucuga ucgauaccua ugggaaacca gacgaaauuc gcguagaguu ggcgagagaa     1560

cucaagaaaa acgccaagga gagggaggaa cugacaaaau ccauagcacg uaauacucgg     1620

gagcaugacg aaauccgaca guuacugaga accgaguuug gaaugaugaa cguuucuagg     1680

aaugauauua uccgcuacaa gcuauaugag gaacucaagg acaacggcua caaaacacug     1740

uauagcaaug aguacauccc ccgggaaaag auuuucucaa aagagauaga uaucgaacac     1800

gugauuccuc aagcuagacu uuuugacgau aguuugucca acaagacccu ggaguacagg     1860

gccaucaaca uugaaaaagg caauaagacg gcuuaugacu ucgucaagga gaaauacggg     1920

aacgauggac ucgagaaguu ucugaaucgc ugcgaaacuu uauucaaaga caagcgaaca     1980

aaacugcgga aacuuaagau ggaggaaaaa gauaucccag aggguuucau agaccgugau     2040

uugagaaaca cccaguauau cagcaagaaa gccuuugcaa ugcucaauga aauuucuagg     2100

cgcguggucg ccacuacagg cucaaucacc gacaagcugc gggaggauug gcagcuagug     2160

gaccugauga aagagcucaa ccugccgaag uacgaaaaac uugggauggu ugagauguuc     2220

gaagacaagg auggaagaaa gauuaaaaag aucacggacu ggaccaaaag gaaugaucac     2280

cgacaucacg cuguggacgc guugacagua gcauuuacua aggaugucuu cauacaguuu     2340

uauaacaaua aaaacgccgc uuggaugccc gccaguaagg agcaugcaaa uauuaccggc     2400

aucaaggccc gcuacuucga gaacgguaaa gcucuggccc cuaugccauu agcacaauuu     2460

cgggcggaag cuaagagaca ccucgagaau cugcuuguga gcauuaaagc caagaacaaa     2520

guuaucacau ccaaugugaa caggacuaag aacaagaaug gggaaaaccg uaaacugcag     2580

cagaccccca gaggccaguu gcaccucgag acagucuaug gaucucaucg gcaauacgca     2640

accaagauag aaaaagugaa ugccgcuuuc gacguaacua agaucgccac ggugucgaaa     2700

cuggcauaca gaaacgcucu acugaagagg cucgaggccu uuggcaauga uccuaagaaa     2760

gcguucacag ggaagaacac ccuggagaaa aauccacuuu auuuggacga aaaccagacu     2820

gagaugguuc ccgaaaaggu ccagaccgug gaguuugaaa caguguacac cauucgaaaa     2880

ccgaucgauc cuaaucugag cauugacaag gucguugaug ugaagauccg guccguguua     2940

gagaaacgcc ucaaggagua uggaggugac gccaaaaagg cuuucguaaa ccuggaugaa     3000

aaucccauau ggcuuaacga ggcaaaaggc auuucaauca agagagucag uaucaggggg     3060

auuucuaaug cccaaagccu gcacgagaaa aaggacaagg acggaaaccu aauccucggc     3120

aaagaagguc ggaagauucc aguggauuuu guuaauacug ggaacaacca ucacguggcu     3180

auauacaaaa agccuguccu ggacaaagug ggccagguug uguuggauga agagggaaau     3240

cccaaguaug agcuggacga aguagucgug uccuucuucg augccgugac acgagcaaac     3300

uuaggccagc caaucauuga caaggauuac agacgcucag agggguggca guuucucuuc     3360

agcaugaaac aaaaugaaua uuuugucuuc cccaacgaca agacgggauu uaauccuaaa     3420

gagguugauc ugcuuaaccc agagaauuac gcgcugaucu cuccgaacuu guucagggug     3480

cagaaguuug ccuacaaaaa uuauguauuc cgucaucacc ucgaaaccac uauuaaggac     3540

acauccagua cccugaaggg uaucacaugg acugauuuuc ggagcucuaa aggccuagac     3600

aagauaguga aagucagagu ggaccacauc gggcagauug uuuccaucgg agaguacuga     3660


<210>  2891
<211>  3663
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2891
augaagagaa uccugggccu cgaccuuggg agcgccucca uuggaugggc ucugauagag       60

gaagaugaga aagguaucag gauuaucgca uugggcucuc ggauuauccc cuacaacggg      120

accgaaggac aggacuucgc caagggcgcg ggugagucac gcaauaguuu acgaacacaa      180

gcucguacua cgagaaaagg guaugauagg uaccagcugc ggcgcaagua ucuaauaaac      240

gugcucgaga aaaaucugau gaugccuagc gaagaccuua gaaagaugcc aaagaugaac      300

cuguggcagu ugagguccaa agccgucacc gaguacaucu cucggcagga acucggacgc      360

cugcugcucu gguuaaauca aaagcgaggc uauaaaucgu caagaagcga ugcaaaccug      420

gacaagaaag auacagacua cguuaaggag gugaagagua gguuugauaa aauuaaggag      480

aaaaaucaga cuaucggcca guauuucuac gaaucccuuc agugcgacga guacuuucgg      540

guaaaggaaa acguguuccc gcgugaggcc uauauugcug aauuugaugc aaucuguucu      600

cgccaaaaag accacuuggg gcugagcgag gaacuaauag ccgagauuag aaaugagauc      660

auuuacuauc agaggccccu caagucccag aaaggacugg ucucagugua cgauuucgaa      720

gguaagugga ugaaccagaa uggcaaggag cuuuuuaucg ggccuaaagu ugcucauaag      780

aguagcccac uguuccaauu ggccaaaauc ugggaaucug ugaacaauau uaagcucucc      840

accaaauaug gagacacaau agagcugacc uuagaagaga agaagcagcu ggucgaguuu      900

cucgacaaca augaaaaacu uacuacagcg gcacuguuca agaucuugcg gaaagauaag      960

aaagaguaca uucuacccaa gcagcuggaa aagggccucc aagguaacau caccaaaugc     1020

aagauagccg acauucuggg gucaaaauau aaccagcuuc ugcaguugaa ucucgagaag     1080

caggaagugg gaacgccuug uuaccuguac acuaaaagca ccggcgaggu auuaggcgag     1140

aagccacugc aauauaucga uaagaguguu gaaaaagagc uauuuuacca gcucuggcac     1200

acaaucuauu cuauuuccga cgaagaugag ugcuguaagg cucugcagaa acgauucgac     1260

aucgaugccg agacugcagg gcgccuugcu aagauugacu uuagcaaaua cggauucggu     1320

aacaagucgg ccaaagcgau aagaaagauc uugcccuauc ugauggaagg cgauaaguac     1380

ucugacgcca ugugcuaugc aggguacaau cauuccaacu cacucaccaa agaggaaaau     1440

cacgagagga agcugaacga uaaacuuaag cuguugacca aaaauagucu ccggcagccu     1500

auuguggaaa agaucaugaa ccaaaugauc cagcugguca auaccauuau agacaaguac     1560

ggaaaaccag acgagauccg cuuagagcug gcuagagaac uaaagcagag caaagaggaa     1620

aggaacgaug ccgacaaggc uauguccaaa cgucagcggg agaauaagga agccgagaag     1680

aaacucgagg aacauggcau ucgagcaacg cacaacaaua uccugaagua uagacuuuuc     1740

gaggaaaugg gugagaaaaa gcacgaagcc auuuguaucu acugcgggaa cccgauaucu     1800

uuuagcgcug cgaugaaugg cgaugaggug gacguggagc auaucauucc caggucaaaa     1860

uuguucgaug acagucaauc caacaagacu cuggcacacc gcaaguguaa cgccaauaaa     1920

aacgauauga cagcuuauga cuuuaugaag accaaaagca agcaggauuu cgacaaauac     1980

gucgaacggg uuaaucagcu cuaugccgau agaaucauuu cuaggucaaa guacaacaaa     2040

cuguuaauga gugaggacga uaucccuagc aauuuuauag accgccagcu gcgagaaucc     2100

caauauauug caacuaaggc ccgggagauc cuucagacaa uuugcuacaa cguguggucu     2160

accucgggaa cgguaaccgc ugaguugcgu caucucuggg gcugggacga uaucacaaug     2220

aaucugcaga ugccaaagua caaagaacua gggcugacug agauggugga auggaccagc     2280

gaccacggaa agaacgucca cuccaaagag gugauaaagg guuggacaaa aagagaugac     2340

cauaggcacc augccaucga ugcacucacu auugcgugua ccaagcaagg cuucauccag     2400

cgcuuuaaua cacugucaag uucuaagacc cgggacgaua ugcagaaaga acuugagaag     2460

ugcagcauuc aguucagaga gaaaaggucc uugcuggaaa aguauaucau aucacaacga     2520

cccauuagcg uuucugacgu ggcugaggcc gucucccgca ucuuaaucag uuuuaaagca     2580

gggaagaagg uggccacuau uggaaaacgg aagguaaaag gcaagguugu gcagcagggu     2640

aucgucgugc cuagaggggc ucucagcgaa ggcucugucu acggaaaaau uaggacgaua     2700

gugcguaaga ugccaguuaa guaucuguuc gagaaccccg aucuuaucuu uaaaccguac     2760

auuaaggccc uggugaaaga acgcuuggca gaguucgaca augauaacaa gcaggcucuc     2820

gccucccuga aaaagaaucc uguauaucua gacaaggaga aaacaaagcu gcucgaauac     2880

ggcacaugcu uuaaagauga guaugucaag aaauacgacg ugaaccccaa uuucacuaag     2940

aucgcggacg uuauugaugg gaaagugaag caaauccugg aagaccggcu uucaaaguuc     3000

ggagguaaac caaaggaggc cuuuaaagau gucgugaagg acggcaaaac cuugaagugg     3060

uacgaagaug aggggcugga gagaccuaua aggaguguac gauguuuaac aggacucagc     3120

gcugugguuc ccgucaagaa agacgaaaac ggcauggaua ucgguuucgu gaagccaggg     3180

aacaaucacc auguggcaau uuaugucggc gccgacggaa aaggccagga gcacguuugc     3240

accuuuugga acgcugugga acggaagaaa uacgggcugc ccaucauuau caaggaugcc     3300

aaugaggugu gggacaagau aacuauggca ggagaugacg uauauccuca gucguuccuu     3360

ucccugcuac caaaaccgga cuggacacuc aagcugucua ugcagcaaaa cgagauguuu     3420

auuuugggca uggaagagga ugaagucaaa uucgcgaucg agagcaagga cuacaaacug     3480

uuaucagauc accucuaucg cgugcagaag cugaguuccu cugacuacaa uuuuauccau     3540

caccuugaaa cggauguuca gaagaaaacc augacuuaua gagugaggag ccuguccggu     3600

uuguucgccc uccggcccuu uaagguccga auugacuguc uggggaacau aaucccuuca     3660

uga                                                                   3663


<210>  2892
<211>  3669
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2892
augaagaaaa gaauccuggg ccucgacagc gggaccaacu cccuuggaug ggccgugguc       60

gauagggacg agaauaacca guacucucug auuaaguaug gugauuugau auucgaagag      120

ggcguuaaaa ucgaaaaggg gguggaguca aguaaagcug cagaacggac aagccaccgc      180

gccuuacgaa agcuguacuu ucguagaagg cuacggaaga uugagguacu caaagugcug      240

gucaaguaua aucuuugccc ccaagugucc gaggaagaac ugcauuugug gcacacucgc      300

aaacucuacc cucagaacaa ggaccugcug caguggcaga gaacggauga aaaagcggga      360

aagaauccau auaucuacag guaucggugu cuccaugagg aguuagacau gucuguuucg      420

gcuaaccgca ugaccguggg ccgagccaug uaccaccugg cacaaagaag ggguuuccuu      480

ucaaaucggu uggauaacga agaggacgaa aaucagauug gggccgucaa gugcggaauc      540

agcaaccuga cagaggaaau acagaguucc ggcuguaaau accuaggcga guauuuuuac      600

aagcucuaua augagcaugg gaacacugug cgucugcgca ccagauacac aucuagggag      660

uuccacuaua aaaaggaguu uuacgcuauc ugcgaaaaac agaaacuuga uaaaaagcug      720

auugaggaau uggcaagcgc ccucuauuuc caacggccgc ugaaguccca gcgacacacc      780

guaggacgcu guacuuuuga gcccaauaaa ccuagaugcg cugacucaca uccagccuac      840

gaggaguuca ggauguuaag uuuuaucaac acaauuaagg uucagggucc cuacgauacc      900

gaacugcggg ugcucaauac ggaggaacgc gacagcauca cuaaacuuuu cuguagaaag      960

ucuaaaccua acuuugauuu cgaagacaua gcgaaggagc uggcaggcag gaacaauuau     1020

ugcuacauug gggacgucgu ggauaaggcc uauaaauuua acuaccguau gucccaggga     1080

gugucaggcu guccaaccac aacuuauuug aucaaugucu ucggucaaga cuggcagacc     1140

gggauugcug agagcuacca ggccaucgau acaaagcuag gacaaaagag uguugacgca     1200

augaucgaug acguguggaa cguacuguau ucuuucucca gcaaggauaa acuccgggac     1260

uuuggccgaa agaagcugca gcuuggcgau aaagaagcug acaccuucgc caauauuaag     1320

cugacgcaca aauuugcguc guugucucuc aaggccauaa gaaaaaucaa cauuuuccug     1380

aggauggggu uaguguacuc ccaugcaguc acucuggcua auauccccac aauaguggga     1440

accgauauuu ggcagcgcaa gcggaacuuu auccuagccg agcuccugcc ucuuaucgaa     1500

aauuucaacc caaaggagac uaaauugcag gagacacugg acuuuugcgu uaaggacuuc     1560

cuccaaaaua acuuugaacu gaaaaauggu gcuguggaua aacuuuacca cccgucaaug     1620

auugaggccu aucccgacgc aggccugaac aguaauggga ucuugcagcu cggaagcccu     1680

agaaccaacg ccauuaggaa cccaauggcu augcgcuccc ugcacgaaau acgaaaaguc     1740

aucaaucagu uacugaagga uaaaauuauc aaccaggaca cggagaucca uguggaauac     1800

gcgcgggagc uaaaugaugc aaacaagcgu aaggccauuu cuauauggca aaagagccag     1860

gagaagcucc acaaaaagua uguagaagag aucaccaauc uguacaaaga agagacaggc     1920

aaggcuauug uucccacuga caccgauauc cuuaaguuca aauuguggga ggaacagaac     1980

cauauuuguc uguauacagg ucagcaaauc gggauagccg aguuucucgg cgcaacuccu     2040

aaguacgaca ucgaacacac cauuccacag ucagugggag gcgauaguac agacaugaau     2100

cugaccuuau gcgauuccag auucaacagg gagaucaaaa aggcccagcu gcccacucag     2160

cuugggaauc augcugacau uuugguccgc aucgccggau ggaaaaacag cuaugaaaag     2220

cucucuaagg auauagaccg gauuagaacg cacucaggua uggcaaaaga caucaaggau     2280

aggcugauuc aaaaacgaca ccuacugaag cucgagcgcg acuacuggcg gggcaaauac     2340

gaaagauuua caaugaauga gaucccggag ggguucagua ggcgucaggg agugggcaua     2400

ggucugauca gcaaguaugc ggggcuuuac uugaaguccc uguuccauga uaaaaacgaa     2460

cgcaauaagg gccacgucuu ugugguuaaa ggaaccacua ccgcugaguu ccggaagaug     2520

uggggcuuac agaacgaaua ugagaaaaag ucuagaaaua accaugugca ccacugcauu     2580

gacgccauca caauugcaug uaucgggaaa caugaguacg cccaaauggc ucaguauuac     2640

cacgaugaag ccgaguaucu cuaccaucac ggaauaaagc cuauuuuucc caagccaugg     2700

aaaaccuuca cugaagaccu gcuugaucug gagcauucgu uguuuguagu ccaccacagc     2760

ccuaacaaua uggcaaagca ggcuaggaaa cgaaucauca cauccacggg ucagcauugg     2820

gcccaaggcg acucagcgca cgggagucuc cauaacgaua ccuacuaugg agccauugaa     2880

cgggacggcg agaucaagua cgugguucgc agagcugugu cuagcuucga gcagaugucc     2940

gaugucgacu caauugugga ugacguagug aaagaaacug uuaggaaggc acuggagggu     3000

aagaauuuua aagaagccgu caacgcuccc auauauauga auaaggagaa aggggugcua     3060

aucaagaaag ugcggugcua cgccaagagc auuaagaaac caaucgacau ucgaacacac     3120

agagauucuu ccaguaagga auauaaacgc aaguuccaug ucagcaacga caucaauuac     3180

cugauagcaa ucuaugaggg caccguuaaa ggaaagguga agagggauuu ugaggugguc     3240

aaaaacauug aagcggccaa uuucuuuaag cguucucagg accggagaga uuaccccaca     3300

aucguaccug agaaauccau uaagcacggc uucccacuca aaacuacccu gacgaacggg     3360

accaugguga uccuuuacga agagaauaag gaagagauag acuuuacaaa gaaagaggau     3420

auuuguaggc gcuuguauaa gcugguugga augucaagua ugaucgugag cggcugcgaa     3480

uacggugucu uaucgcuccg acaccaucag gaggcuagac cguccaaaga caucaagucu     3540

aaaaacgggg ccuucaagaa uggagaagca cugcggcccg cuauuacuau gcuucacacc     3600

caauucaacg cccuggugga gaauauagau uuuaacaucg acguuacagg caacauugag     3660

cuaaucuga                                                             3669


<210>  2893
<211>  3669
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2893
augaagaaaa gaauccuggg ccucgacagc gggaccaacu cccuuggaug ggccgugguc       60

gauagggacg agaauaacca guacucucug auuaaguaug gugauuugau auucgaagag      120

ggcguuaaaa ucgaaaaggg gguggaguca aguaaagcug cagaacggac aagccaccgc      180

gccuuacgaa agcuguacuu ucguagaagg cuacggaaga uugagguacu caaagugcug      240

gucaaguaua aucuuugccc ccaagugucc gaggaagaac ugcauuugug gcacacucgc      300

aaacucuacc cucagaacaa ggaccugcug caguggcaga gaacggauga aaaagaggga      360

aagaauccau auaucuacag guaucggugu cuccaugagg aauuagacau gucuguuucg      420

gcgaaccgca ugaccguggg ccgagcuaug uaccaccugg cccaaagaag ggguuuccuu      480

ucaaaucggu uggauaacga ggaagacgag aaucagauug gggcagucaa gugcggaauc      540

agcaaccuga cagaagagau acagaguucc ggcuguaaau accuaggcga guauuuuuac      600

aagcucuaua augaacaugg gaacacugug cgucugcgca ccagauacac aucuagggag      660

uuccacuaua aaaaggaauu uuacgccauc ugcgagaaac agaaacuuga uaaaaagcug      720

auugaagagu uggcuagcgc acucuauuuc caacggccgc ugaaguccca gcgacacacc      780

guaggacgcu guacuuuuga gcccaauaaa ccuagaugcg ccgacucaca uccagcuuac      840

gaagaguuca ggauguuaag uuuuaucaac acaauuaagg uucagggucc cuacgauacc      900

gaacugcggg ugcucaauac ggaggaacgc gacagcauca cuaaacuuuu cuguagaaag      960

ucuaaaccua acuuugauuu cgaggacaua gccaaggagc uggcgggcag gaacaauuau     1020

ugcuacauug gggacgucgu ggauaaggca uauaaauuua acuaccguau gucccaggga     1080

gugucaggcu guccaaccac aacuuauuug aucaaugucu ucggucaaga cuggcagacc     1140

gggauugccg aaagcuacca ggcuaucgau acaaagcuag gacaaaagag uguugacgcc     1200

augaucgaug acguguggaa cguacuguau ucuuucucca gcaaggauaa acuccgggac     1260

uuuggccgaa agaagcugca gcuuggcgau aaagaggcag acaccuucgc uaauauuaag     1320

cugacgcaca aauuugccuc guugucucuc aaggcgauaa gaaaaaucaa cauuuuccug     1380

aggauggggu uaguguacuc ccaugccguc acucuggcaa auauccccac aauaguggga     1440

accgauauuu ggcagcgcaa gcggaacuuu auccuagcug aacuccugcc ucuuaucgag     1500

aauuucaacc caaaggagac uaaauugcag gaaacacugg acuuuugcgu uaaggacuuc     1560

cuccaaaaua acuuugagcu gaaaaauggu gccguggaua aacuuuacca cccgucaaug     1620

auugaggcuu aucccgacgc cggccugaac aguaauggga ucuugcagcu cggaagcccu     1680

agaaccaacg caauuaggaa cccaauggcc augcgcuccc ugcacgagau acgaaaaguc     1740

aucaaucagu uacugaagga uaaaauuauc aaccaggaca cggaaaucca uguggaguac     1800

gcucgggagc uaaaugaugc gaacaagcgu aaggcaauuu cuauauggca aaagagccag     1860

gaaaagcucc acaaaaagua uguagaggaa aucaccaauc uguacaaaga ggaaacaggc     1920

aaggccauug uucccacuga caccgauauc cuuaaguuca aauuguggga ggagcagaac     1980

cauauuuguc uguauacagg ucagcaaauc gggauagcug aauuucucgg cgccacuccu     2040

aaguacgaca ucgagcacac cauuccacag ucagugggag gcgauaguac agacaugaau     2100

cugaccuuau gcgauuccag auucaacagg gaaaucaaaa aggcacagcu gcccacucag     2160

cuugggaauc augccgacau uuugguccgc aucgcuggau ggaaaaacag cuaugagaag     2220

cucucuaagg auauagaccg gauuagaacg cacucaggua uggccaaaga caucaaggau     2280

aggcugauuc aaaaacgaca ccuacugaag cucgaacgcg acuacuggcg gggcaaauac     2340

gagagauuua caaugaauga gaucccggaa ggguucagua ggcgucaggg agugggcaua     2400

ggucugauca gcaaguaugc agggcuuuac uugaaguccc uguuccauga uaaaaacgag     2460

cgcaauaagg gccacgucuu ugugguuaaa ggaaccacua ccgcggaguu ccggaagaug     2520

uggggcuuac agaacgaaua ugagaaaaag ucuagaaaua accaugugca ccacugcauu     2580

gacgcuauca caauugccug uaucgggaaa caugaauacg cacaaauggc ccaguauuac     2640

cacgaugagg cugaauaucu cuaccaucac ggaauaaagc cuauuuuucc caagccaugg     2700

aaaaccuuca cugaggaccu gcuugaucug gaacauucgu uguuuguagu ccaccacagc     2760

ccuaacaaua uggccaagca ggcaaggaaa cgaaucauca cauccacggg ucagcauugg     2820

gcucaaggcg acucagccca cgggagucuc cauaacgaua ccuacuaugg agcgauugag     2880

cgggacggcg agaucaagua cgugguucgc agagccgugu cuagcuucga acagaugucc     2940

gaugucgacu caauugugga ugacguagug aaagagacug uuaggaaggc ucuggaaggu     3000

aagaauuuua aagaggcagu caacgccccc auauauauga auaaggaaaa aggggugcua     3060

aucaagaaag ugcggugcua cgcuaagagc auuaagaaac caaucgacau ucgaacacac     3120

agagauucuu ccaguaagga guauaaacgc aaguuccaug ucagcaacga caucaauuac     3180

cugauagcca ucuaugaggg caccguuaaa ggaaagguga agagggauuu ugaagugguc     3240

aaaaacauug aggcagcgaa uuucuuuaag cguucucagg accggagaga uuaccccaca     3300

aucguaccug aaaaauccau uaagcacggc uucccacuca aaacuacccu gacgaacggg     3360

accaugguga uccuuuacga ggaaaauaag gaggagauag acuuuacaaa gaaagaagau     3420

auuuguaggc gcuuguauaa gcugguugga augucaagua ugaucgugag cggcugcgag     3480

uacggugucu uaucgcuccg acaccaucag gaagccagac cguccaaaga caucaagucu     3540

aaaaacgggg cuuucaagaa uggagaggcc cugcggcccg caauuacuau gcuucacacc     3600

caauucaacg cucuggugga gaauauagau uuuaacaucg acguuacagg caacauugaa     3660

cuaaucuga                                                             3669


<210>  2894
<211>  3678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2894
augagcaaga gaauccuggg cauugacgug gggaccaacu cccucggaug ggccgucguu       60

gauaggacag acugcgauaa uuacacucuu gugaaacggg gugacguaau auuccaggag      120

ggcgugaaca ucgaaaaggg gaauauugag aaaucucgcg cuucagaacg aacgggacac      180

cguaguacca gaaagcauua uuuuaggcgg cgcaugcuga aaaucaaggu cuuggagauu      240

uuaguggaac accaacugug ucccuaccua agccaugagg agcuccacca guggaugaca      300

agaaagguuu auccuacuaa cgaagauuuc aggcuguggc uucggaccaa ugacgcaaac      360

gaucagaauc cauaccauga ccgccacgug ugccugcacg agcgauugga ucucagacug      420

caggaaaaca gguaucggcu gggccgugcc cucuaccauu uagcgcaacg cagagguuuu      480

cuguccaaua ggcuugagca gaacucuaaa acagacgaga ccggggcugu caagucggau      540

aucucacagu ugaaugaaga gaugaaaaag gccggaagcc aguaucuggg cgacuacuuc      600

uacuggcuau aucaagaaua cggcaaccag gugacucucc ggauacgaua uacagaucgc      660

aaacagcacu accagaagga guuugaagca aucugucaaa gacagcagcu gagugacgac      720

cuuguagaua agcugaggcg ggccauuuau uuccaacgcu ccuugaaauc ucagagagcu      780

gggguuggaa agugcaccuu ugagccggac aaacccaggu gugcacucag ccauccucgu      840

uacgaggagu uccggaugcu guccuuuuua aauaacguga aggucaaagg uccaucagau      900

gacagucugc gaccccucag cccuucugag aaggaaaaga ucaagccacu uuuccugaga      960

aaguccaaag ccaauuuuga uuucgaggac auugcuaagg ccaucgcggg caaagggaag     1020

uaugcaugca uaagggauaa agaaggagcc cagaagcccu accaguuuaa cuacgcuuug     1080

ucacaaggcg ugagcgguug uccugugacg gccaagcuaa uugcagucuu cggggagagu     1140

ugggagcgca accugguuaa ugcuuaucgg ucuagaaacg acaugaggaa auccgaugaa     1200

gagaucguga augaaguaug gaacgugcuc uacagcuucu cgucuaagga gaaacugcgc     1260

gccuggggaa uggaaaaacu uggccuggac gaggcgugcg ccggcgaguu ugcagauauu     1320

acuuugccaa ccgcuuucgc cgcucucucc cugaaagccg ucaauaagau cuuaccguuu     1380

cugcgacagg gguucaucua uucacacgca gugaugcuag ccaacauucc cuacauaauc     1440

ggaauggacc gguggaauga accucgucag agacaguuua uuauggacaa ccuccaagag     1500

cugcuugaua auuucacagc uagugacaag gcguugcagc agacucugga uuuuuguauc     1560

aaagacuucc uccugaacaa uuaugaacuu gcaccaggua aggccgauag gcuguaccac     1620

cccagcauga uagagaccua uccugacgcu aaagccgaug gccaggggau uuaccaauug     1680

ggauccccag caacaaacgc cauccgcaau cccauggcua ugcggucucu ccauauccug     1740

agaaggguug ugaaccaguu acugaaggag aaaaccauug acagcgaaac ggagguccac     1800

aucgaauacg cccgagagcu aaaugaugca aaccgccggu gggcgauuaa ccaguggcag     1860

agaaagcaag aaacucagag gaaggacuau gcugagaaaa uacguaaggu guacgccgau     1920

gaguuugaaa aaguaaucga gccgacacag gaagaacucc ugaaguauca acuuugggaa     1980

gaacagcagc auuugugccu guacaccggc gagcagauug gucucacuga cuucaucggg     2040

ccuaaucccc gcuaugauau cgaacacaca auuccacaau caaugggcgg agacaguacc     2100

cggaugaacc ugacguuaug ugagagaacc uacaauagga caguuaaagg caaccgaaug     2160

ccuucccagc uggcagaaca ugagagcaua cuuacucgga ucaagcccug gaaagacaag     2220

uaugaagagu ugaccgugaa gauuaauaaa cuccgcacac acccagggau ggauaaggcc     2280

ucuaaagaca acaucauuag aaagaggcau cugcuagagc uggaacggga uuacugguuu     2340

cagaaauacc agcgauucac uaugaccgag guccccgaag gauuuucacu cagacaaggu     2400

acaggcaucg ggcugauagg aaaguaugcu ggccuuuucu ugaagagucu guuccaccac     2460

gugaaagagc gcaauaggaa gcaggucuac gugaucaaag guccuauuac cgccgaguuu     2520

cgucgguuau gggggcucca gaaccuguau gaacaaaaga gcagagacaa ucaugcacac     2580

cacugcaucg augcuauugu uaucgccugc auaaacccag cggaguacgc caggauggcu     2640

uccuauuacc gccaggaaga ggacuuccga cuuaauaaag gcaacaagcc gguguuuccc     2700

aagccuugga cuacguucac acaggaucug caggcauugg aagccgagcu ccugguaguc     2760

caugagacca gaaauaaccu aacuaaaaag gcucggaaaa gggugcgcac caagggaggc     2820

aauguucugu cugaaucgga cagcgcccgu gggucccucc acaacgauac auauuacgga     2880

gcaauucaaa aagacggugu gaucagauac gucgugcgga aggcgcuguc aagucuuucu     2940

gagaaggaug uaaaagacau uguggauaag accguuaggg aaaaagucca ggccgcuauc     3000

caggagaagg gcgccaaaga agcauuggcu gggacuauau auaugaacga ggagaaggga     3060

aucacaauua aaaaggugcg cuguuuuacg aagaucgcca gcccacuggc aauucgaaga     3120

caucgggacg ccuccgcuaa agaauacaag aggcccuauu acgugaccaa ugagucaaac     3180

uaugucaugg gcaucuacga agguugcaua aaagagaaga cuaaacgcga guucauuuua     3240

guuaauaacc ucgaggcggu gagacagcug aaggccgcaa ggaagcaagg ggugguaagc     3300

ucuauccuuc cuccagucuc cgcuaaaagu caguuucccc ugaaguaucg gcuauacaca     3360

ggcacccucg ugcuguugua cgagaauagc ccggaagaga ucgacuucac aaaccagguu     3420

gauauuugua aacgucugua uaaggugacu ggauuaucuu ccaugaccgu cacgggcaau     3480

caguacggga ccauccaacu ccgcuaucac caggaagccc gacaggccaa agaccugaag     3540

gcucagaacg gagcauacaa ggagggcgag gaacauagac cugccauuau gaugcuucac     3600

acacaauuua aagcgcuggu ggaggguacu gauuucagga ugaauauauu gggggaaauc     3660

gagccacucc ggaaguga                                                   3678


<210>  2895
<211>  3690
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2895
augggcgaca gaaaguacaa ccuggggcuc gauaucggaa ccagcuccau ugguuucgcc       60

gcuguggacg agaauaacca gcccauaagg gucaaaggca agacagcaau cgggguucgg      120

cuuuuugaag agggaaaaac ugccgcggau cgccgaggcu uccguacgac cagaaggcgg      180

cugucucgca gaagguggcg gauuaauuug uuaaacgaaa ucuuugacgc ucaccuggcc      240

gagguggauc cuacauuccu agcacgccuc aaggaaucaa aucgaaguaa ccuggacccg      300

aaaaagagcu uucaagguuc ccuucuguuc ccagagagaa aggauuauca guuuuacgag      360

gaauauccca cuauuuacca uuugaggaaa gcccucaugg agaaggaccg gaaauucgau      420

auccgugaaa uauaucuggc uguacaccau aucauuaagu accgcgggaa uuuucugaac      480

ggaaccccua ugagaucuuu caaaguggag aauaucgagc ucaacacauu auuugaccag      540

cugaaucagc uuuaugcaga aauuguccca gauaacgagu uggccuucga ccuggcucaa      600

guggccgaug uuaaggacgu gcuaucguca accacuaucu acaagaugga caaaaagaaa      660

cagcucguca agaugaugcu gcuucccgcg agcaauaaag cacugcagag ugaaaacaag      720

aaaauaguga cacaguuugu aaaugccauu uugaacuaca aguucaagcu cgauguucug      780

uuacaagugg agaccgacgc ugauuggucc cugaaacuca acgacgaagg cgccgaugac      840

aaacuugagg aguucacggg cgaucuggac gaaaauaggu uggagaucau ugaucuacug      900

cagcggcucc acaacugguu uucucugaau gaaaucacua aggacgggaa cagccuuucc      960

gcagcuaugg ucgagaagua ugaaaaucau caccaccauc ugggauugcu caaaaaggug     1020

aucgagaacc acccugaugc caagaaagcg aaggcccuga aagagaccua cacagcauau     1080

guggguaaga cugacgacaa aacccagaau caggaugacu ucuacaaggc ugucgaaaag     1140

aacuuagaug acucaccaga ugccaaaggc auuaagcgac ugauacaacu agaccaguuu     1200

augcccaaac agcgcacagg gcaaaaugga gcuaucccuc aucagcucca ccagcaggag     1260

cuggaucaaa uuaucgaaaa gcagaguaaa uauuacccau uccuugccga gccgaacccc     1320

aauguuaaga gaaggaagga cgcaccuuau aaauuggaug aacugauagc cuuuaagauu     1380

ccauacuacg ugggcccccu cguaaccccu gaggagcagg cucagaacaa agaaaaugug     1440

uucgcgugga ugaagcggaa agcagccggu ccaaucacgc ccuggaacuu ugacgagaag     1500

gucgaucgca uggaaagcgc uaauagauuc aucaggcgua ugacuacaaa agacaccuau     1560

cuguuugggg aggaugugcu uccggccgag ucuaugauuu accaaaaguu cguugugcug     1620

aacgaauuga acaaucucaa gaucaacgga cggcaccugu ccuuaaaaga caagcaggac     1680

gucuauaaug aucuguuuaa acagcagaag acugugucaa uuaaagcacu acaaaacuac     1740

uauguaacaa agaagaaagc cgcuaccgcc ccuacgguug gcggccucgc agaccccaag     1800

aaauuccuga gcagucuuuc uaccuacaua gauuuuaaga auauguucgg ggagcgagug     1860

aacgacccac aguuccagga agauuuggag cagaucgucg aaugguccac aauuuuugag     1920

gacagaggaa ucuucaaagc gaagcugcaa gcucucgguu ggcugagcga gaagcagauc     1980

cagcaauuag uggccaaaag guauaagggc ugggggcgcc ugucgaaaaa guugcuuacu     2040

ggacucaaaa augcagaagg cuacucuauu cuggaugaga uguggcgguc caccgaaaac     2100

uuuaugcaga uacagucaag accugaguuc gccgcucuaa uccagcaagc caaugaaaag     2160

caguuugagg guaacgaccc cgauaauguc ugggagaaca uugaaaauau ccugggggac     2220

gcauacacaa guccacagaa caagaaagcu auucgccagg ugguuaaggu gguacaagau     2280

aucgagaaag ccgucggcaa ucccccugaa aagauagcga ucgaguucac uagggaagcc     2340

gcugcaaacc cacagcgaac ccagagccgg cuccguacac uggagaaacu uuaugagucc     2400

gccgaagagg ugguugacgc uggauugacc gccgaacugg cagaguuuaa ggagaacaaa     2460

cauguguuau cugauaagua cuaucucuac uucacucagc ugggcagaga cgucuauacg     2520

ggggacacaa uuagccuuga uaagcugaau gacuacgaug uggaccacau cuugccgcaa     2580

ucauuuauua aagaugacag ucucgauaac aggguacuga ccauccgcgc ggugaauaac     2640

ggaaaguccg acaauguucc cgccaaaaug uucgguaaga aaaugggcag cuuuuggcgg     2700

caucuacugg auaacgggau gauaucuaag agaaaguaua auaaccucau uacugacccu     2760

gauaauaucu caaaauacgc ucagaaggga uucauuaaca ggcagcuggu cgaaaccagu     2820

caagugauca aacuuacagc caauauauug aacggcaucu acgacaagga caccgagauu     2880

aucgaagugc cagcaaaaau gaauagccag augcgaaaga uguuugaucu ggucaagguu     2940

cgcgagguga acgacuauca ccaugcuuuc gaugccuacu uaacuauuuu caucgguaau     3000

uaucucuaca aaugcuaucc caagcugcgg ccuuacuuug uguaugacaa cuucaaaaag     3060

uuugggaaca aagaagauau aggccacaag agauucaauu uucuuggaaa gauugagagg     3120

gagaaaaagg uagucgcacc agaaacaggc gagauccugu gguccaacgu ggcccccaau     3180

gaaacgauca aacagauuaa gaaaguuuac aacuacaagu ucaugaucgu gucucgugag     3240

auuaccacuc gccgggcuga acuauuuaau cagacagucu auccgaagaa cuacagaggg     3300

aaacucauac cuaucaagga ggacaggcca accgaucugu auggagguua cucgggcaau     3360

acagacgcgu auuuggccau uguggcacug gaggauaaaa agaaagggaa guacuucaaa     3420

guugugggaa uccccacucg agcugccgcc aaguuagaaa agcucaaaca acaggacagc     3480

cagcaguauc ugcaagcacu ucacgaggua auugcuccuc aguuuaccaa guccacgaaa     3540

aagggcauca aaaagaccga guucgaaaua guccuggaua aggugcauua ccggcaguug     3600

gugcaggacg gucccgucaa aaugaugcuc gggucaagua cauacaagua uaacgccaaa     3660

caacugguuc uaucugaaaa ggcgcuguga                                      3690


<210>  2896
<211>  3696
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2896
augaucacca ggauucuggg ccucgacaca gggacuaaca gccuugccug ggcugugguc       60

gauagggacg caaauggaaa cuacacgcug guuaagcggg gugaugugau auucaccgag      120

ggcguaaaaa ucgaaaaggg gauugagucc ucuaaagccg cggaacgcac agcucacaag      180

gccuugcgag ugcauuauuc acguagaagg uuacacaaaa ucgacacucu gaagguccua      240

guggagcucg aucugugccc cuaccuuagu aaugaacagc ugaacuugug gaaggccaaa      300

aagaccuauc cucucacaga cgauuuuaug cuguggcaac ggagcggauc cgcagaggag      360

aauaacccau acuauugucg ccauagaugc cugacccaga aacucgacuu aacugaucag      420

ucucagaggu uccugcuugg ccgggcuuuu uaccacuugg cacaacgccg agguuuccug      480

ucgaauagac uagacacauc agccgauagc gacgaaagug ggaagguuaa auccggaauu      540

gcugagcucu cuguggaaau ggagaaggcc ggcugugagu uucugggcga auauuucuac      600

cagcuuuaca agcagcaugg gaacaccauc aaacugagga gccgguauac ggaucgugag      660

gaacacuaca agaaagaguu uuuggcgaua ugcgaaaagc aggagcucga cccgaaaugg      720

gucgaggcac ugcaacgcgc caucuauuuc cagagacccu uaaaguccca gaggaaagga      780

guggguaagu guacuuuuga accuaagaaa ccacggugcg cugagucaca ccccgacuac      840

gaagccuucc gaaugcugug uuuucucaau aacguaaaga uucagacccc uaaugauaca      900

agucuucgcc cacugaacga ggacgaaaga gagaaaaucu ggcccuuguu cuuuagaaag      960

agcaaaccua auuucgauuu cgaggacauu gcaaaggcuc uagccggcaa gaacaauuau     1020

gcgugguaca aagaugccgg ggaaaagcca uuuaaauuca acuaucggau gcaacaggga     1080

guuucuggcu gcccggugac ugcacagcug aucgacauau uugguuccga uuggcaaacc     1140

gcuauugccg agacauacac cgcugucgaa aagaaaaacg ggacgaagac uaucgacgag     1200

auggccgaug acguguggaa ugugcucuac ucauucagca guaaggauaa acugaaggca     1260

uuugccacag aaaaacuuca gcuggacgag gcuucugcga ccaaguucgc aaacgccaaa     1320

uugccccaug gauuugcuuc ccucagccug aaggccauuc gcaauaucuu accuuuccug     1380

agacagggcu uuaucuaugc acacgccguc caucuagcua agauuccaga cauaguuacu     1440

aaagccgugu ggaacgaugc agacaagagg gagaugaucc ucucgggccu gauggaaauu     1500

aucgagaauu acaaccccaa agaucguggg guagaaggaa caauagacuu cuguauuaag     1560

ucucaccuuc aggaugugau cgacuugcgg ccuggugcgg cugaugcccu guaucaccca     1620

uccaugaucg agaccuaccc cgacgcaaaa cucaauaaga ugggcgucuu ucaacugggg     1680

ucaccgcgaa cgaacgccau uagaaauccu auggcuauga ggagucuuca uauccugcgc     1740

agcgugguua accaguugcu ccgggaugga auuauagaca gaaauaccga ggugcacguc     1800

gaauaugcca gggagcugaa cgaugcaaau aaacgcaagg cuaucgccga cuaccagcga     1860

gaacaggaga agaaacggaa gcaauaucgu gaugacauua ucgcguuaua caaauccgaa     1920

ugccauaagg agaucgagcc cacagacacu gauauucuga aauuccagcu augggaggaa     1980

caggaccacc ucugucugua caccggcaca cagauaggua ucugcgauuu ucuuggggac     2040

aacccaaagu ucgauauuga acauacugug ccuagaucug uaggcggaga cagcacccaa     2100

augaauuuga cacugugcga ugccaccuuc aacaggcagg uuaagggcgc ucagcucccc     2160

ucacagcuga guaaccacgc agacaucuua gugcgcauug aucacuggaa auccaagugu     2220

gaggaccugc ggaaacaacu ugauaagauc agaacuuaug ggggaaugga aaaagccaug     2280

aaggacaggu ugauacagaa gcgacaucgc cucaaaaugg aucuggacua cuggcagggu     2340

aaguauagcc gguuuacgau gacagagguc ccagagggcu ucucuagaag gcaaggggcu     2400

ggaaucggcc uagugucaaa auacgccggu cuguuucuca agagucuguu ccacaaugaa     2460

cagaacccca auaaaagcaa cgucuaugug guuaaggggg ugaccacugc agaguuucgu     2520

cgcauguggg gccuucaggg agaauacgag aagaaauccc gguugaauca uugccaccac     2580

uguauugacg cgaucaccau ugccugcauc ggcaaggcug aauaugauaa aauggcccag     2640

uacuaccaug acgaggagcu guuccaaugg agagggacaa ccacuaagcc ucacuuuaac     2700

aaaccauggc cgacauucac ggaagaugua uuagcaauag agcagucucu ccuggucaag     2760

caucuuacca aagacaauau gcccaagcag gcuaagaaaa gggugcgaac ugccacagga     2820

aaguaucugg cacaggguga uaccgcccgg ggcucguugc acaaagacac auacuauggg     2880

gcuauugaac aagauggaga gauccgcuac guucucagaa agccucugaa caaauuugag     2940

aaugaagcgg agcuagaaaa gauuguggac caggccguca aggagaaaau caaggaagca     3000

auagcuggca aaaacuucaa gacugccauc accgagccaa uuuuuaugaa uaaagagaag     3060

gguaucagga uuaacaaggu gcgguauuac guaaaagccc aggaacgacu gaagaucaga     3120

cagcaacgcg aucucagcuc caaagaguau aagcaguucu uuugugugga aaaugaguca     3180

aacuaccugc uugcuauaua cgaaggggag uugaaaggca agaaggagag ggacuucugc     3240

guugucaaua accuggaaag ugcagccugu uuaaaacguu cuaacaaggg aaaagcggcu     3300

ccccucgugc cugagcggag cgaagagaga aaucugccac uucuggccgu gcuaaagagg     3360

ggcacgcagg uccucuucua ugaaaacucc cccgaggaga uucuguuuga uaauccgcaa     3420

gacuugguuc gccgacugua caaagugacc gggaucaaca caaagaagga cgugaaaaau     3480

ggaaagacuu auuuauacgg caacaucgaa cucagauucc aucagaaugc acagaaaaag     3540

gaugacguaa aaaugcaguc aggugucuau aagaacaaug aggugcugcg gcaagggauu     3600

guucuuagcc acaaacaguu uaaggcccug guggaaggag ucgauuucga guuuaacgug     3660

uugggcgaga ucaagaaaaa ggaaaggcac gcuuga                               3696


<210>  2897
<211>  3699
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2897
augaccaaga caagaauccu gggccucgac acugggacga acagccuugg augggccauu       60

guggauaggg acgaggucuc cggucaguac gaacugguug aucggggcga cuugauauuc      120

accgaggggg ugaaaaucga aaagggaauu gagucuucaa aagcuaguga acgcacacaa      180

caccgagcau uacguaagca guauuuuaga aggcggcugc gcaaaaucga gguacuaaga      240

gugcucacuu ucuaccaucu gugucccccu gucagcgagg aagaacuuca ccuguggcau      300

gugaagaaga uuuauccagu ugaaaaucac gaguuuauga ucuggcagag gaccaacgau      360

aaugagggca aaaacccgua cuauuaccgg cauauaugcu ugcacacaaa gcucaaucug      420

acccugauga aagaccgcua uauccucggu cgagccuuau accaccuguc ccagagaagg      480

ggguuccuuu cuaaccgguu ggaucaaucg caggacgaag augacaaggg aacugugaaa      540

acaggcauuu caagccugag uaaggauaug aaugacgcgg gcugcaccua ccuaggggau      600

uauuuuuaca agcucuauuc cgagaacgga aaucgugucc gcaucagaaa ccauuacacg      660

gcuaggaaug aacacuaucu ggccgaguuc gaagcaauuu gucaggugca gaaacuugag      720

cccgagcugg uagaagccuu gcggcgagcu aucuuuuucc aacgcccucu caagucucag      780

agaaaaggug uuggcaagug cacuuuugag ccaaggaaac cccggugugc agacagccau      840

ccugacuacg aagaguuccg caagcugugc uuuauaaaca auauuaaaau ccagaccucc      900

gaugacgaua caaugagacc auuaaacaag gaagagaggc agcguauuaa uucacuguuc      960

uaucggguga guaaggccaa cuuuuacuuc gaggaaaucg cuaaacuccu ugggggacga     1020

aacaauuacu guuauuucaa ggacgagguc caaaaagauu uuaaguucaa cuacagaaug     1080

agccagucug uguccggcug ccccacuacc gcccagcuga ggaauaucuu uggugaagac     1140

uggaaaacag ggauugcgga gaccuaucaa cacucagcaa agaaggaugg cagcuugaaa     1200

acgguggacg aaauggccga ugacauaugg aacguccuau acaguuucuc uuccaaggau     1260

ugucuguaua auuuugcugu uaacaaacuc cagcuggagg aggacaaggc caaaaaguuc     1320

agcaagauca aacuuucgca cggauuugca ucuauuuccc ugaaugcuau ccauaagaua     1380

uugccuuucc uccgcauggg caugauuuac ucacacgccg ugcuguuugc gaacauccca     1440

acuaucguag ggauggacgu guggaaugau gaaaguaaac ggcagcauau uaugacagag     1500

uuaagcgacc uggucuucaa cuaugauccg aagaauagag aaguggaggg aaccaucgag     1560

augugcauua aagacuuucu acucaacaau uacgaucugc ccgccggugc aguuuguagg     1620

cuuuaccacc cuuccaugau agacacuuau ccagaugcua aggccaacaa guacggcgug     1680

uugcagcugg ggucucccgc uacaaaugcc auccgcaacc cuauggcaau gcgaagccuc     1740

caugaacugc ggcacgucgu gaaccuucug uugcguaaag gaaagauuga ccaaaauacc     1800

gagguacacg uugaauaugc cagagagcuc aacgaugcua auaaaaggaa ggcgaucgca     1860

gaccgccagc gggaacugga uaaauuacau aagaaauacc acgacgauau cauuacgcug     1920

uauaaggagg agaccggcaa ggacauaaac ccaacacagg ccgacauccu aaaauuccag     1980

cucugggagg agcaagaaca ugugugccug uacacuggua gacagauugg gaucucaaag     2040

uuucuuggcc ccgauccgga guucgacauu gaacacaccg ucccuaggag ugugggaggc     2100

gauuccacac agaugaauau gacuuugugc gaccgacgcu ucaaccggga ugucaaaaag     2160

gcuaugcugc ccagccagcu cccaaauuau caagagaucc ugccuagaau agagaaaugg     2220

aagaagaaau acgaaaaguu aaccaaagac aucgaucacu guauuacaca uucugggaug     2280

accaaggagg ccaaagacuc aaucauucag aagaggcacc gucugacucu ugaacaugau     2340

uacuggcgca acaaguauag ucgguuuacg augacagagg ugcccgaagg auucagcaga     2400

aggcagggug caggcaucgg guugguuucc aaauacgccg gacucuuucu gaagucucua     2460

uuccacgacc caaccgaucg acggcgcucg cauguguaug uagucaaagg cacuaccaca     2520

gcugaguuua gaaagaugug gggucugcag agcgaguacg agaaaaaguc cagggacaau     2580

cacgugcacc auugcguuga ugccauaacc auugcaugua ucgggaagaa cgaauauaau     2640

aaaauggcgu cauucuacca cgacgaagag gcuuuugagc ggggcgaaug cgagaagccc     2700

cauuucgaca aaccuuggcc aacuuuuaca caagaugugc ucggacugga aaagcgacuu     2760

auugucgugc acaguacgca ggacaacaug ccgaaacaug ccaagaaaua uguaagaacu     2820

ccccgcggca aguucaucgc aaagggggau ugugccaggg gaucuuugca ccuggacacc     2880

uacuacggug cuauaaaaca caaggaugac acagagccuc guuauguguu acggagagaa     2940

cucagcuccu uugauucagu uaaugaccug gauaucauug ucgacgaugc cgugagggag     3000

aaaaucaagg aggcagugaa aggcaagagc gggaaggcuu ucaaagaagc ccuugcgaac     3060

gucuacauga auaaggagaa agacauucgc aucaagaaag uucgaugcua ugugccaucu     3120

gugaagagac ccauagacau ucggcaucag agggauaugu cccgcuuuga cuacaagcgu     3180

caauuccacg uagccaacga uggaaacuau augauggcua ucuacgaagg cgucgugaaa     3240

gguaagcuga aaagagacuu cgaguugguu aagaauaucg aagcagccag uuauuuuaaa     3300

gugagccagg auaacgaguc uuucuccuca auugucccug agaagaguag caauggguuu     3360

ccacucaagg cugugcugaa aaucggcacc cauguucuac uguacgaaaa cacagccucg     3420

gagauugacu ucuccgaucc cguggaccuc acucggaggc uguacaaggu agucggacuu     3480

ucuagcgcaa uaaucaauaa cuguucauau ggcuuuauua ccuugcgcuu ccaccaggaa     3540

gcgcgacagg ccaaaaaucu gaaguuaacg aaaggggugu uuaagaacgu ggauaauuac     3600

agaggagcua ucauucucua ucacacccaa cugaaggccc uugucgaggg cuacgacuuc     3660

gauaucaaca uacuggguga aaucacaagu aaacaguga                            3699


<210>  2898
<211>  3705
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2898
augcccaacu ucuggagcau cuuuaccgag aaucugcaga cauaccucau gaagacuaga       60

auuuuaggcc uggacacggg gaccaacucc uugggauggg ccguggucga uagggacgaa      120

aauaaccaau auacaccuau augccggggu gauuuaaucu ucacugaggg cguuaaacag      180

gaaaagggga uugaguuuuc ucuggcugca gaacgcaccu cauaccgagc cagucguaga      240

cacuauuuca ggcggcgccu aagaaaagug gagguacuca aggugcuggu caaacauaaa      300

cuuuguccac cgcugagcga cgaggcguug aauagguggc acacaaagaa agaauacccc      360

cucgaugagg acuuuauccu guggcagcgg accaacgaag cugauaauaa gaacccuuau      420

caugaccgcc acauuugccu gcaucgaaaa cucaauuuaa aggauuccca gacugacaga      480

uacacacugg gaagggcccu uuaucacuug acccaacggc guggcuuccu gucuaaccgc      540

cuagaucaga augaggaggc agaagagacg gguaaaguga agucggccau cucagaacuc      600

agcaaggaga ugaaagaagc ugggugugag uuucugggag acuacuucua ccuucuguau      660

agaacugagg gcaacagggu ucggauacga agucgcuaca ccgauagaga aaagcacuau      720

uugcaggagu uuaaagaaau cugcagggug caggagcucg acgaagaccu ggcaaaggcc      780

uuagagaaaa caauuuucuu ucaacggcca cugaaguccc agcgccaggg cgucgggaaa      840

uguacuaugg agaagggaca uucuagaugc gcugugagcc acccccucua cgaagaguau      900

aggcuucugu guuacuugaa uaacauccgu auuaagaccc cuuccgauga ccggcuacga      960

ccacugaaug ccgaagagag agaaaagauc acaccccucu ucuauaagga gaaaaacuuu     1020

gauuucgaga agauagcgaa aaagcuggca gguaagaccc cuuucggcug gauuagggac     1080

gccgaagauu ggccauacca guuuaacuac cgcaugacgc aagaggcucc ggccugcccc     1140

acuacagcac agcuuaucaa aguauucggg gaagacugga aggcugcccu ggcggagacc     1200

uauacuaugu cagccaaaca ggauggaaca uugaagaccg aggacgaaau uuguacggau     1260

aucuggaaug uucucuacag uuuuagcucu aaagagaagc ugaaggaguu cggccuuaaa     1320

aagcugcaac uagacgaagu gcaggcaaag gcuuuuuccg aaauccggcu caccagagau     1380

uucgccucac ugagccuuaa ggcuauuagg aaaauauugu uuuuccugcg cacaggugag     1440

gacuauagua aggccaucuu ucucgcaaac auuccuacua ucgucgggga ggaaauaugg     1500

aaugacauug agcgacgguc ucgucugcuu uacaaggugg aucuggccuu gagaccacag     1560

gaagagaggg acgcugagga acaucgcacc aucgcggagc ggaucaaagc agccguggcu     1620

gccgaagucc ccgaugcacc uauugaccuc cuguaucacc cauccaugau cgaggccuac     1680

cccgaugcua agagaaacga aagguucgac ugcuaucagu uaggaagccc gaaaacaaau     1740

gccguucgaa acccuauggc aaugcgcucg cugcacaugg ugcggaaggu aauuaaucau     1800

cuacucagaa aacacauaau cgaugagaag acugaaauuc auaucgagua cgcgagggag     1860

cugaacgacg cuaauaaacg ucaagccauc gcagauuggc agcgcgaacu uucuaagcgg     1920

cacaccgccu acgcucagaa cauuagacag uuguauaagg ccgagacagg ccuggugaua     1980

gaacccacca aagcagacau ccucaaguac caacuguggg aggagcagca gcaugucugu     2040

uuauauacug gcaaaaagau ugggaucgcu gauuuucugg gagccaaucc aaaauucgac     2100

auugaacaca cgaucccuag gucccgaggu ggcgauucaa cacaggagaa ccuuaccuug     2160

ugcgaaagua aguuuaaucg ggacauaaag cgcgacaaac uccccacuga gcuggcgaac     2220

uacgaagaga uccuagccag aauugagcac uggaagaaac aaugcuaugc ucugaagcuc     2280

aaaagggaug ggauccggac ccauagcgga cuggaaaagu ccauuaagga cucucgaauc     2340

agacgcaggc accuuguggg cuuggaguac gguuaucuga aagggaagua cgaucguuuc     2400

acaaugaaag aaguuccaga gggcuucagc cggagacagg gagcaggcau aggguuaauu     2460

ucaaaguacg ccggacucuu ucugaaaagu cuuuucaagg accccaagaa uccuaacagg     2520

uccaacgugu augucgugaa aggugcuauc accgccgaau uucgcaagcu guggggcuug     2580

cagcaggcag augaggcgaa aagccgagag aaucauacuc accacuguau ugacgccuac     2640

acaaucgcuu gcauagggcc augugaauau gccgcacucg cugccuacua uagaucugau     2700

gaggaguuca aguacggacg gaggcgcgaa aaaccgcaau uugaaaagcc cuggccuacg     2760

uucaccgagg accugcuaaa acugcaggag gaacuccuga ucguacauca gacuacagau     2820

aaacuuggca agcgugacag acggaaaguu aagaccccaa gggguaaauu uuugacaggg     2880

ggcgauucag cacgcggacg acugcaccaa gagacuuauu acggcgccau uaacuacgac     2940

gggaauauca aguauguggu cagaaaaccc uuagauaguc ucaccgaaaa ggaccuggac     3000

aagauugugg augagacggu caugggaauc gugaaaggug cuguugaggc gaagggcagc     3060

cuuaaagaag cccuggcaga cgggauauac augaacaagg agaaaggcau ucuaaucaag     3120

cgggugagga ucuucuccga uguaucugaa ccuauuaauc ucaagcagcu gcgcgacguc     3180

ucgaccaaag aguauaagag acauauccac uugaggaaag augaaaacua ccuguuugcu     3240

auuuaugagg gaauagacaa gaaaaagaau aagaaacaga cagagacugc caccguggau     3300

uacuuagacg ccgcuaaguu cuauaaagau agcaagcggc gugacaaagc aaacucccuc     3360

guuggugauu acucaaagaa gaauaaacug ccacuuaagu gcauccugaa aaagcgccag     3420

augguguugu uuuacgaaca uagucccgag gaaauucgau ucaaagacaa gaaagaccuc     3480

ucuagaaggc uguauaucgu cacaagcauu gccaaggaug ggcgggugaa guuucuacac     3540

caccaagcgg cuacugccac cggcauccag uccucaccga gcccuuucac gaacgacgga     3600

gagcaucagc cauacuaucg ccugucugca uccaauauaa aaguacuugu ggccggcauc     3660

gauuucgaca uuaacauccu cggggaaauu acacccaagc uguga                     3705


<210>  2899
<211>  3711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2899
auguacuucu auaagaacaa agagaauaag cugaacaaaa aggugguccu cggccuugac       60

cuggggaucg ccagcguugg auggugcuug accgauauuu cccagaaaga agacaauaag      120

uuucccauaa ucuuacacgg ugugagacug uucgagacag uagaugacuc ugaugacaag      180

cuacucaacg aaacuaggcg gaaaaagcgc ggccaacgac guagaaauag gcggcuguuu      240

acgcgcaaaa gagauuucau uaaguaccuu aucgacaaca auauuaucga gcuggaauuu      300

gauaaaaacc cuaagauauu ggugaggaau uucaucgaga aguauauuaa cccauuuuca      360

aagaaucucg agcugaagua caaaaguguc accaaccugc cgaucggguu ccauaaucuc      420

cggaaggcug caauuaacga aaaauauaag uuagacaaaa gcgagcugau cgugcuuuug      480

uacuuuuauc ugucccuacg cggagccuuc uuugauaauc ccgaagacac aaagucuaag      540

gagaugaaca aaaacgagau agaaauuuuc gauaagaaug agucgaucaa aaacgcggaa      600

uuuccuauug acaagaucau cgaguucuac aaaauuucag gcaagauacg aagcacuauc      660

aaucucaagu ucggucacca ggacuaccug aaagaaauua agcagguuuu ugagaaacag      720

aacaucgauu ucaugaauua ugagaaguuu gcuauggaag agaaaaguuu cuuuuccaga      780

auaaggaacu acucugaagg gccaggaaau gaaaagagcu ucuccaagua uggccuuuac      840

gccaacgaaa auggcaaccc cgagcugauu aucaaugaga aagggcaaaa gaucuauacc      900

aaaauuuuua agacauugug ggaaucaaaa aucggaaagu guaguuacga caagaaacuc      960

uaucgggcac cuaagaacag cuucucugcc aaaguguuug auauuaccaa uaagcugacu     1020

gacuggaaac auaagaacga guacauaucc gaacguuuaa aacgcaagau ccugcucuca     1080

agauuccuua auaaggauag caaaagugcu gucgagaaga uucugaaaga agagaacauc     1140

aaguuugaga acuugucuga aaucgcauac aauaaagacg auaacaagau uaaucuacca     1200

auaaucaacg ccuaucacuc ccugacaacc auuuucaaga aacaucucau caauuuugag     1260

aacuaccuga uuagcaauga aaacgaccuu ucgaagcuga ugucuuucua uaaacagcag     1320

uccgagaagu uguucgugcc caaugagaaa gguucauacg aaaucaacca gaauaacaau     1380

guacuccaca uauuugaugc uaucaguaac auucugaaua aguucagcac gauccaagac     1440

aggauucgga ucuuagaggg cuauuuugaa uuuuccaacc ugaagaaaga uguuaagucu     1500

agcgagauau acucagaaau ugccaaacua cgagaguuua gugggacuuc cagccucucu     1560

uucggagcgu auuacaaguu uaucccuaac cugauuucag agggcaguaa aaauuacagc     1620

accaucuccu augaagagaa ggcacuucag aaccagaaga auaacuucuc ucacucgaau     1680

uuguuugaga aaacaugggu ggaggaccug auagccagcc caacugucaa gcgcucccuc     1740

agacagacca ugaaccugcu uaaagaaauc uucaaguacu cagagaaaaa uaaccuggag     1800

auugaaaaga ucguggugga ggucacaagg aguucuaaua acaaacauga acggaagaag     1860

auugagggua ucaauaaaua ucgcaaggaa aaauacgagg aguugaagaa aguuuaugac     1920

cucccgaacg aaaauaccac gcuguuaaag aagcuguggc uacucagaca acagcagggg     1980

uacgaugcuu auagccugag gaaaauagag gccaacgacg ugauuaauaa gcccuggaac     2040

uacgauaucg accacaucgu accucguucc auuucauuug augacagcuu cucuaaccuu     2100

gugaucguca auaaauugga uaacgcaaag aaauccaaug accugagugc uaagcaauuu     2160

auugaaaaga uauacggaau cgagaaacuc aaggaggcca aagaaaacug gggcaauugg     2220

uaucugcgga acgcgaaugg caaggccuuc aacgauaaag ggaaguucau uaaguuauac     2280

acuaucgaca aucuggauga guuugacaac agcgauuuca uuaaucgaaa ccuuucugac     2340

acauccuaua ucaccaaugc auuggugaac caucucacuu uuucaaauag uaaauacaag     2400

uauagcguug ugucggucaa cggaaaacag acauccaauc ugagaaacca gauagcuuuc     2460

guggguauca agaacaauaa agaaaccgag agggaaugga agcgcccaga gggcuuuaag     2520

ucuauuaaca gcaaugacuu ccuaauccgg gaggaaggga aaaacgaugu aaaggacgau     2580

guucugauua aagacagauc auuuaaugga caccacgccg aggaugcuua cuucaucacg     2640

auaauuaguc aguauuuuag guccuucaag cgcaucgaac gacucaacgu gaauuaccgg     2700

aaagagaccc gugaacugga cgaucuugag aagaacaaua ucaaauuuaa ggagaaggcc     2760

ucuuucgaca acuucuugcu gauuaaugca uuagaugaac ucaacgagaa acugaaucaa     2820

augagauuua gcaggauggu cauaacaaag aaaaacacuc agcuuuucaa ugaaacccug     2880

uacuccggca aguaugacaa agguaagaac acaaucaaga aaguggagaa guugaaccuc     2940

cuggauaauc gcacugacaa aauuaagaaa aucgaagagu uuuucgauga ggacaagcua     3000

aaggaaaacg agcugaccaa acuccauauu uuuaaucacg acaagaaccu guacgaaaca     3060

cuuaaaauca uauggaauga ggucaagauc gagauuaaaa acaagaauuu gaacgaaaag     3120

aauuauuuca aauacuuugu gaacaagaaa cugcaggagg ggaagaucuc auucaaugaa     3180

uggguuccca uuuuagauaa cgacuuuaaa aucauacgga agauuagaua uaucaaauuc     3240

agcagugagg aaaaggagac cgaugagauc auuuucucuc aguccaauuu ccucaagauc     3300

gaccaaaggc agaacuuuag cuuccauaau acucuguacu gggugcagau auggguauau     3360

aaaaaccaga aggaucaaua cugcuucauu ucgaucgacg cccgaaacuc aaaauuugaa     3420

aaggaugaga uuaaaaucaa uuacgaaaaa cuuaagacgc agaaagagaa gcugcagaua     3480

auuaacgagg aaccuauccu aaaaaucaau aagggcgacc ucuucgagaa cgaagagaaa     3540

gaacuguuuu auauugucgg acgcgaugag aagccacaga aguuggaaau caaauacauu     3600

cugggcaaga aaauaaagga ccaaaaacag auccagaagc ccgugaagaa auauuucccg     3660

aauuggaaga aaguuaacuu aacauacaug ggggagauuu uuaagaaaug a              3711


<210>  2900
<211>  3717
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2900
augaccaagg ugcugggccu cgaccuuggg gccgcuagcg ucggaugggc aaugaucgag       60

gaagaggaaa aaucccacaa gauucugggu uugggcucua gaauaauccc cuacgagggg      120

acagaaggaa acgauuucgc caaaggcacu ggugagucaa ggaaugcguu acggacgaag      180

gcucgcaccg cccgaaaagg guaugaccgu uaccagcuga gaaggaagua ucuaguugau      240

acacucguga agaacgagau gaugccugac gaauaccugc ggaaacuucc aaagaugcaa      300

cugugggagu ugcgcaguaa agcaguaacu accgaaauua caaagcagga gcucggaaga      360

cuguuucugu ggcucaauca gaaaaggggc uauaagagcu cccggucuga ugccaacuua      420

gacaagaaag auaccgagua cguggucgaa gugaagucgc gccaugagaa aaucaaggaa      480

ucaggcuuca cuauugggca guauuuuuac agcgagcuga aaaguaauga auacuuccga      540

guuaaggaga acaucuuucc gagagaggcu uauauagaag aguucgacgc aaucugcaaa      600

caacagaaga ggcaccuuac agauaaguug auuucccgga uccguaauga aauuaucuac      660

uaucagcgcc cccugaaauc ucagaaggga cuugugagcg ucuguagauu ugaggguuuc      720

uuuaaaaagg gcauaaacgg gaaagaauac uucgugggac cuaagguagc cccaaagucc      780

ucaccccucu uucaaaccgu uaaaauuugg gagaauauca acaguauuag gcugagcgac      840

cggaauggca aggagaucau gcuuucucug gaacagaaaa agugcaucuu caaccauuug      900

gauaauaacg agaaacucac gccuaaggac cuguuuauua uaaucggugu gaagaaagaa      960

aauuguuucg ucaacaagca guuagagaaa gggauucagg gaaacgugac uaaguccacc     1020

auccugaaau gcuucggcga aaauaacgau uaugacaagu ggcucaaacu ugaccugaau     1080

auaauuguga aaaaggauac auuuggcuac uuguaugaca aaaagacugg ggagaucauc     1140

ggagagaaac uaauuaagca cgucgauuca gcuguugaac gagagccacu guaccaacuc     1200

uggcauacca ucuacagcau uaacaauaca gacgaaugua gugccgcgcu gcagaacaaa     1260

uucgguuuug auucugagac cgcagacaaa cuuuccaaua uagauuucgg caaggccggg     1320

uuuggaaaca agagcgcuaa ggugauccgc aaaauucugc ccuauuugau ggagggcgac     1380

gauuacucgc agucuauguc cuaugccggu uucaaucacu cagacagucu cacgaaggau     1440

gaaaaccuga aaagaaaguu acuggacaag cuaaggccua ucgagaaaaa uagccuccgg     1500

caaccaaucg uagaaaagau ucugaaccag auggugaaug ucgugaacgc aauaaucgac     1560

aaauacggga agccggauga gauucgcauc gaacuugcua gagaguugaa acagaauaag     1620

gaggaaagga acaagacuga caaagauaug uccaagcguc agcgggagaa cggcgaaauu     1680

ucuaaacgac uggccgagua uggacucaga gcgacaagga auaacaucau aaaguggcgc     1740

cuguacgaag agaucgacaa ucaagauaaa aagcuaaacg ccauuugcgu uuauuguggc     1800

cagcccauca gccugaccga ggcaauuaag gggaaugaag uggacgucga gcacaucaua     1860

ccuaaaucaa aguuguuuga ugacagucag uccaacaaaa cucucgcuca ucggagaugc     1920

aauagcacaa agggagauau gaccgccuac gacuucaugg aaaacaaacc agcucaggug     1980

uuuaaugccu acauugagag gguaaacaug cuguaugaua auaagaucau ucgcaaaguu     2040

aagcgagaca aguuacugau gcccgaaucu aaaaucccug auaacuucau agaccggcaa     2100

cuacgugagu cacaguacau cgcaaguaag gccagagaga uucuccagac gaucuguuau     2160

aaugugugga gcaccuccgg uacagucacu gcuaggcugc gccaccuuug gggcugggac     2220

gaugcgacca ugucuuugca gcugccaaaa uaccgggaac ucgggcugac agagauggug     2280

gaaugggagu cggagcaugg aaagcaaaaa cacagcaagg aagucauuuc cgacugguca     2340

aagagagaug accacaggca ucacgcagug gaugccuuaa cuaucgcuug caccaaacag     2400

ggcuuuauac agcgauucaa cacacugaau aguucuaaga ccaaagagga cauggaauuu     2460

gagauugcca aguguagcau cgaauaucgc gagaaccuuu ccaaauugga gaaguacauc     2520

auugcacucc ggcccauccc gguuucagaa guggaggaug ccguagcuaa cauucugauc     2580

agcuucaagu cugguaaaaa ggucgccgug aaagggaaga gaaaaauagg caagagggga     2640

aauaagaaaa uuauccagac ugacauuauc auaccucaug gcucccuaag ugaagagagc     2700

guuuauggga agaucaaaac gauugaacgu gagaagcccc ugaaauaccu cuuccaaaac     2760

ucucagcuga ucuuuaauaa caagauuaag auccuugugg aggaacgcuu ggcaacauuc     2820

gagaaugaua ccaaaaaggc gcuggcuucc uuaaagaagg ucccagugua ccucgacgaa     2880

gagaaaacua uaguacugga auaugcaacc ugcuuuaagg aggaguacgu gaaaaaguau     2940

gauguugaca caaacuucuc aaagaccgau aaagucauug acgacaagau caaagaaauc     3000

cuucagcacc ggcugggaaa guuugguggc aaagccaagg agaaggaagc cuucaaagau     3060

gugcaacagg gggacaagac uauuaaaugg uacgaggaug agggauugga aagaccuaua     3120

cauaguguga ggugcuuuac aggccucagc gcuaucgucc ccguuaagaa agacgagaau     3180

gguauugaua ucggguucgu gaagccaggc aacaaucacc acauugccau cuauucggac     3240

aaggauggaa accgauacga acauauaugu acguuuuggc acgcagugga gcggaaaaag     3300

uaugacaucc ccguagucau uaaagauucc aaugaaaucu ggaacaagau uuggucuaaa     3360

ccugagggca gcuacccaca gucauuccug gacaagcuac cgcaggagaa ucugacccuc     3420

gaucugagua ugcaacagaa cgaaaugcuu aucuugggga uguccuacga ggacauaaag     3480

acugcuauug acaauucuga uuauaaacag cugagcucca acuuauaccg ccuccagaag     3540

cuggccaacg cgaauuauau guuuagacau caccuugaaa cacaaaucau cgacgauaac     3600

aauucaaaag ccagcaggcg guucauuaac auccacaguc ugaaggcucu auuugcaugg     3660

aaucccuaca aagugaacau aaauugccuc ggagacauuc agaccaacuc ucgauga        3717


<210>  2901
<211>  3720
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2901
augaccgaga agagaaaauu cgacaucagg gaaguguacc uggccauuca ccauauaguc       60

aaguaucggg gcaacuuucu caauagcgcu acaguugauu ccuucaaaac uucuaacauc      120

gacuuuacgu cacaguucga ucgccuuaau gagcuguacc gacaagugau uuuggaagaa      180

cccuuucaga ucaacaugaa ucagguagac aagaugacca acaaauuacu ggauaaugac      240

gcacuaaagc ucgauacaca gaagcaagug gccaaacugc uuccugucau uuauaacgac      300

aagccagugg auaaacagua cacucgucug gcgaccgaau uuaguaaggc uaucuugggg      360

uauaaaacaa agcucgacgu uauacugaau cuggauaccc agaacgccaa ggacugggca      420

aucagacucg augacgagga cauugaugac aaauuaccgg cccuggugga gaaucuugau      480

gaaagcaggc aggagaucgu cacuauuauc cgggacuugu acgcucaaau aacacugaac      540

gcaauugugc ccaagggaaa aucccuaucu gaaucgaugg uagauaagua uaaugaccac      600

aaagaucauc ucgaccugcu uaugggucug auccacgagu ugggcgauga cucagccaag      660

gggauuaaac uccgcgaggc uuacacccag uacguuggaa agagcggcga uaagacgcug      720

aaccaggacg acuuuuaugc cgcgaucaaa aagaauuuag augaagacag uaaaagaucc      780

ccuaagaucc agaggcugau ugagcaagca ucuuucaugc caaaacagcg gacuagcgcc      840

aacgguguga uaccccauca gcuccaccaa cuugaacugg aucgcaucau ugagaaccag      900

gggcgauacu auccuuucuu gaaggaacca aauuccaaca cccacaagcc caauggaggc      960

aaauacaagc uagacgagcu ggucgcuuuc agaaucccuu auuacguggg cccacucaua     1020

acaaaacagg aucaggagaa gacuucaggg gccgacuuug cauggaugau uaggaaaccg     1080

ggacaaggug gcgaaaucac ccccuggaac uucgaucagg agguggaccg gauggcuagu     1140

gccaauacau uuauccgucg caugaccacg aaggauagcu aucuggucgc ggaagacguu     1200

cuuccugauu cuucccugau uuacgagaag uucaaagugu ugaacgaacu caauaugcug     1260

aagguaaacg acagaagguu aucaaucagc cagaaacagg aucuguucaa uaaccuauuu     1320

aagaaacaaa agacuauucg gacaaagaaa cuccagaccu acauacgaac uaagugggag     1380

cugccaagug ugaugaucuc ugggcuuucc gaccccgaca aauucaauag cucguuggcc     1440

acauauauug auuuucgcga cgucuucgga gagaaggugg augacccuaa cagacaggca     1500

gauuacgaga aaaucaucga guggucuacc guuuuugaag acaggaagau uuaucaggcu     1560

aaacugaauc aacucgauug gcugacggag ucccagucaa aggcccuucu gaccaagcau     1620

uacacaggcu ggggucgguu gaguaaaaag cuccugacug gguuaaaaga ccagaacgga     1680

gauagcauac uggaccagcu auggaagacc aaugauaacu ucaugcaaau ccagucccgc     1740

gacgaauuug cuaaacagau ucacgaugag aacgccaagc aauuccauga ggcaaauaac     1800

guggacgaca uccucgauga cgccuuuaca ucuccacaga auaagaaagc uauuagacag     1860

gucgauaagg ugguacagga cguugugaaa gcggucggcu augcacccga uaagaucgcc     1920

auagaguuca cuaggagccc gcaagaccgu ccucagcgga ccuuuucacg acagagacag     1980

cugcaaagua cauaccuuaa cguggcuaaa gccuugauga aguccaaucu gaaccaggaa     2040

cucaagagcg ucaucgauuc ucagcagacc cugacugaca aauuauaucu guacuucacg     2100

caacuuggcc aggauaugua cacagggaag gaaauuaauu uugacgaguu ggugaacuau     2160

cagaucgauc acauucuccc ccaagcauuc aucaaagacg acucacugga uaauaggguu     2220

cuagugagug ccccaauaaa caaugcuaag agcgacaacg uaccugucaa aaaguucgga     2280

gccaagaugg guuacuuuug gaaacagcug gcagagaauc accucauuuc caagcgcaaa     2340

cugaacaauc uuaccacuga ucccgacaag aucggcaaau ucaccgcgca gggguuuauu     2400

caucggcagu ugguggaaac aucucaaguu aucagacugg uggcuaacau auuaggaaac     2460

gaguauggcc acgaugguac cacuaucauu gaagucacag ccaagaugaa ucaucagaug     2520

aggaaagacu ucgaucucau caagauucgc gacgugaacg auuaccacca ugcaauggac     2580

gccuaucuga cggcuuacgu aggggauuau cuuuaccugc gauacccaaa guugcggucg     2640

uauuuugugu acggcgacuu caaaaagcuc aaagaucaga gccugaagau ccguaauuuu     2700

aacuuccuac acgaccugac caaugacgag gccggagaua aaauaguuga ccaggaaacu     2760

ggcgagauua ucugggauaa gcacgagucc gucaagcaac ucaaaaaggu guaucauuac     2820

aaauuuaugc uggugucaca ggaagucuau acaagacagg acgcacuuuu caaccagacc     2880

aucuaccccg cuagugaugc cgacaagagg aaauugauuc cuaucaagaa uaacaagcca     2940

guugaugugu augggggaua cucugguaau guggacgcgu acauggccau uguacgcaua     3000

cacggcaaaa aggaggauaa auauaagguc guggggguuc cgaugcgggc uguggcagac     3060

cugaaaaagg ccgaaagcaa gggaagagau aacuacuuaa augcugucca ugaggugcuc     3120

aaaccccaau uuacaaagaa aaagaaagac aggaagacug gcgaaaucac cgagaugaug     3180

gaugacuucg acguucugcu ugguaaggug uauuaccgac agcugauugu agaugggaac     3240

aaaaaguuua ugcuaggcuc cucaacguau caguacaaug ccaaacagcu cguccugagc     3300

gacaaggcaa ugcaaguguu gucuaaagau gacaagcuga aaaaccagga ugagaaucag     3360

aacuuaaucg acguguauga ugaaauucuc gagaaggucg accaauacuu cgaacuguac     3420

gauaucaaca aguuccgcca gaaacuucac gacggacgga agaaauuuau agagcugccu     3480

guugauaaug acuucaacgg caagaaauug aucuccuaug ggaagagagc gaccauuauc     3540

aguauucuca auggacugca cgccaacgcu acaaugagca aucuaaagua ccuggguauc     3600

ucuuccccau uuggcaugcu ucaggugccc aacgggauaa uucucagucc ugccucaagg     3660

aucugcuauc agagcccaac uggacuguuc gaacguaaag uaaaguuguc ggaccuguga     3720


<210>  2902
<211>  3738
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2902
augaucauuu acuucauauu ugagaagauc gugaacacca uucuggucau gaaaaagauc       60

guuggccucg accuugggac aaauagcauu ggaugggccc ugaucaacgc uuauauagau      120

gaauacaaua aagagcacuu guaugguauc gaagcaugcg gcuccagaau uauccccaug      180

gacgccgcga uuuuagggaa cuucgauaag ggaaauucua ucucacagac ugcugacagg      240

acgaguuacc ggggcauacg ccgacugcgu gagagacauc uacucaggcg ggaacgccug      300

caccggauuc uugaucuguu ggguuuucuc ccuaaacauu auagcgacuc ccugaacagg      360

uacgggaagu uccugaauga uaucgagugu aagcucccau gggugaaaga cgagaccgga      420

ucuuauaagu uuauuuucca agaaucguuu aaagagaugu uagccgauuu cacagaacac      480

cauccgaagc ugaucgcaaa caauaaaaag guacccuacg acuggacuau cuacuaucuu      540

cggaagaaag ccuugaccca gaagauuuca aaagaggagc uggcuuggau acuacucaac      600

uuuaaucaga agcgcggcua cuaucagcug cgaggcgaag aggaagagac accuaacaaa      660

cuuguggaau acuauagccu gaaggucgag aaaguggagg auagugggga aagaaaggga      720

aaggacaccu gguacaaugu ucacuuggag aacgguauga ucuauaggcg gacuuccaau      780

auuccacucg auugggaagg caaaacaaag gaguucaucg ugaccacgga ccuggaagca      840

gacgggucuc ccaaaaagga uaaagaggga aacauaaagc guagcuuucg cgccccuaag      900

gacgaugacu ggacuuuaau uaaaaagaaa accgaggcug auaucgacaa gaucaaaaug      960

acagucggcg ccuacauuua cgauacucug cuccaaaagc cagaccagaa gaucagaggu     1020

aaacuuguga ggaccauuga acggaaguau uacaaaaacg agcuguauca gauauugaag     1080

acacaguccg aguuccacga agaacuacga gauaaacaac uguacaucgc gugccucaau     1140

gagcuguauc ccaacaauga gccucgcaga aacucaauua guaccaggga cuuuugucau     1200

cuuuucaucg aagauaucau uuucuaccag cggccacuua agagcaagaa aucuuugaua     1260

gacaauugcc cguaugagga aaaccgcuac aucgacaagg aguccgggga gauuaaacac     1320

gcaucaauca aguguauugc caaaagccau ccccucuacc aggaauuuag acuguggcag     1380

uucaucguaa auuuaaggau auaucguaag gagacggaug uugacgugac ucaagaacug     1440

cuaccuacag aggcugauua cgucacccuc uuugaauggc ugaacgagaa aaaggagauc     1500

gaccagaagg ccuucuuuaa auauccaccc uucggauuua agaaaacuac aaguaauuac     1560

cgguggaacu auguggaaga uaagccuuac ccaugcaaug agacccacgc acagauuauc     1620

acgcgacuug gcaaagcuca cauucccaag gccuucuugu cuaaggaaaa agaggaaacc     1680

cuguggcaua uccucuauuc cauagaggac aagcaagaga uugaaaaagc gcugcacagc     1740

uuugccaaca agaauaaccu uucggaggag uucaucgaac aguuuaaaaa uuucccgccu     1800

uuuaagaagg aauacggcuc uuacuccgca aaagcuauua agaaacuguu gccccucaug     1860

agaaugggga aguauugguc aaucgagaac auagauaacg gaacaaggau ccgcauuaau     1920

aaaaucauug acggugagua cgaugaaaac auccgggaga gagugaggca gaaggccaua     1980

aaucugacug acauuaccca uuuccgcgcu uuaccacugu ggcuagccug uuaucucguc     2040

uacgaucgac acagugaagu uaaggacauc gugaaaugga agacaccuaa agauaucgac     2100

cuguaucuua agagcuucaa acagcauucc uugcggaacc ccauuguaga gcaagugauc     2160

acugagaccc ugcguacagu cagagacauu uggcaacagg ugggccacau agaugaaauc     2220

cacauugagc ucgggaggga aaugaagaau ccagcagaca aacgcgcccg gaugucucag     2280

cagaugauca agaacgagaa uaccaaccug agaauuaagg cuuuacugac ugaauuucuu     2340

aaucccgagu ucggaaucga gaacguuagg ccuuacagcc caucacagca ggauuugcuc     2400

cgaauauaug aagagggcgu gcugaauagu auccuagaac ugccggagga cauugguauc     2460

auucucggga aauuuaacca gacggauaca cugaagcgcc ccacccgguc cgaaauccuu     2520

agauacaaau uguggcugga gcaaaaguac aggagcccuu auacuggcga gaugauacca     2580

uuaucuaaac ucuucacccc cgcguacgaa auugagcaua ucaucccuca gucacguuau     2640

uuugacgaua gucugagcaa uaaggucauu ugcgaauccg agauaaacaa gcugaaagac     2700

cgcucucugg gauacgaguu caucaagaau caccauggcg aaaaagugga guuggcauuu     2760

gauaagccag uagaaguucu cucgguggag gccuaugaaa aacuggucca cgagagcuac     2820

ucccacaacc ggucaaagau gaagaaacua cugauggagg acauucccga ucaguucauc     2880

gaaagacagc ucaacgacag uagguauauu ucuaaggugg ucaaaagccu gcuuuccaau     2940

aucgugcgag aggaaaacga gcaagaagcu auaucaaaga auguuauccc guguacaggg     3000

ggaauuaccg aucgguugaa aaaggacugg gguaucaacg acguguggaa uaagauugua     3060

cugccucgcu uuaucagauu aaacgagcuc acugagagca caagguucac gucuauaaau     3120

accaacaaua cuaugauucc auccaugccc cuggaacuuc agaaaggcuu uaacaagaaa     3180

cggaucgauc aucgacacca ugccauggac gcaaucauua ucgccugcgc uaauagaaac     3240

auagucaauu accugaacaa ugugagugcc agcaagaaca caaaaauuac ccgcagggau     3300

cuacagacac uccuguguca caaggacaag acugauaaca augggaacua caaauggguu     3360

aucgacaagc cuugggagac cuucacgcaa gauaccuuga cagcacugca gaaaauuacu     3420

gugucuuuua agcagaauuu acgugucauc aacaaaacca caaaucauua ucagcacuac     3480

gaaaacggaa agaaaauagu guccaaucaa ucaaagggcg acaguugggc gauuagaaag     3540

agcaugcaca aagagacugu acauggugaa gugaaccuca gaaugaucaa gaccguuucg     3600

cugaaugagg cucuuaaaaa gccccaggcc aucgucgaga uggaucugaa aaagaagauu     3660

uuggcaaugc ucgaacuggg guaugacacg aaaaggauca agaacuacuu cgaggaaaau     3720

aaagauacau ggcaguga                                                   3738


<210>  2903
<211>  3741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2903
augagacugg uguaccucgc ccuugcucac augaucaagu ucaggggcca uuuucuguau       60

gagggggacu ugaaagcaga aaacaccgau guccaggccu uauucaagga cuuuguugag      120

gaauacgaua aaacaauuga ggaaagccac cuguccgaga uaacugugga cgcgcuaucu      180

auccucacgg agaagguauc aaaaaguagc cggcuggaaa aucuuauugc ucauuauccc      240

accgagaaga agaacacacu guucggaaau uugaucgccc ucucccuggg ucugcaagca      300

aacuuuaaaa cuaauuucca gcucucugaa gaugccaagu uacaguuuuc gaaagacacc      360

uacgaggagg aucuggaagg ccuuuugggg gaggugggag acgaauaugc ugaucuguuc      420

gucucagcaa agaaccuaua cgacgccauu cuccugagcg gcauccuuac aguggaugac      480

aauaguacca aagcuccucu guccgccucu augguuaagc gcuauaagga gcacaaagaa      540

gaguuggcgg cauuuaagcg auucauaaaa gagaagcucc cgaaaaagua cgaagagauc      600

uuuaaagaug acacuaagaa cgguuacgcc ggguaugugg gagcugacaa gaaacugcgu      660

aagagaagcg gcaaauuagc cacagaagag gaauuuuaca aguaugucaa aggcauucug      720

aauaaggugg agggggcaga uuacuuucuc gacaagaucg auagggagga cuuccuucgg      780

aaacagcgca ccuucgauaa cggauccauu ccacaucaaa uccaucugca ggaaaugcac      840

gcuauauuga gaaggcaggg ugagcacuau cccuuucuaa aggaaaauca ggacaaaauu      900

gagaagaucc ugacguuccg gauuccuuac uauguaggcc cacucgcccg caaaggguca      960

cgauuugcgu gggccgaaua caaggcagau gagaagauca cucccuggaa cuucgacgau     1020

auccuggaca aagagaagag ugcugaaaaa uuuauuacca gaaugacacu uaaugaucug     1080

uacuugccug aggaaaaggu ucucccaaaa cacagccccc uguaugagac uuucaccgug     1140

uacaacgagu uaacaaaggu caaguaugug aaugaacaag gagaggccaa auuuuucgac     1200

accaacauga agcaggaaau auuugaccau guguucaaag agaacaggaa ggucacgaaa     1260

gauaagcugc uaaauuaccu caacaaagaa uuugaggagu uccggaucgu uaaucugacu     1320

ggccuugaca aggaaaacaa ggcuuuuaau gccucuuugg guacauauca cgaucugcgu     1380

aaaauucucg acaaguccuu ccuggaugac aaagugaacg agaagaucau agaagauauu     1440

auccagaccc uuacucuguu cgaggaccgc gaaaugauca gacagagguu gcaaaaauac     1500

ucagauauuu uuacaaccca gcagcucaag gagcuggagc ggcgacacua uacggggugg     1560

ggacgcuuaa gcuacaagcu gaucaauggc auuagaaaca aagaaaauaa gaaaaccaua     1620

cuagacuacc ucaucgauga cggcuaugca aacaggaauu ucaugcaacu gauuaacgau     1680

gacgcccuua guuuuaagga ggaaaucgcu cgggcgcaga ucauugggga cguagaugac     1740

auagcaaaug ugguccauga uuugccugga ucuccagcca ucaaaaaggg uauucugcag     1800

uccgugaaga ucguugacga gcucgugaaa gucaugggcc acaacccgac aaauauuauc     1860

auagaaaugg cucgcgagaa ccagacuacc gauaagggga gaaggaacag ccaacagcgu     1920

cugaaauuac ugcaggacuc gcuuaagaau uuggauaacc ccgugaauau caaaaacgua     1980

gagaaucagc aacuccagaa cgaccggcug uuccuauacu auauucagaa uggaaaggau     2040

auguacacag gcgaaacucu ggacaucaac aaucucucuc aguaugauau ugaccauauc     2100

auaccucaag ccuuuauuaa ggauaacucc cuggacaauc gaguucuuac cagaucagac     2160

aaaaacaggg guaagaguga ugacgugcca agcaucgagg ucgugcacga aaugaaaucc     2220

uucuggucua aguugcugag cgucaaauua auuacacagc gcaaguuuga uaaucucacc     2280

aaagcagagc ggggcgggcu gacugagaac gacaaggccg gauucaucaa gagacagcuu     2340

guggaaacga ggcagauaac aaaacauguu gcucaagugc uggaugcccg cuuuaaugca     2400

aagcacgacg agaacaaaaa gguaauccga gaugucaaaa uuaucaccuu gaagucaaac     2460

cucgugaguc aguuccggaa ggacuuuaaa uucuacaagg uucgugaaau uaaugauuau     2520

caccaugcgc acgacgcuua ccugaacgcc gugaucggca cugcacuacu gaaaaaguac     2580

cccaaacucg ccuccgaguu ugucuauggg gaguucaaga aguacgaugu gagaaaauuc     2640

auagcuaaga gcgacaaaga aauuggaaag gccaccgcaa aauauuucuu cuacucuaau     2700

cugaugaacu uuuucaagaa ggagguaaaa uuugcugacg guacaguggu ugaaaggccu     2760

gauaucgaga ccucagaaga cggcgagauc gccuggaaua agcagacuga uuucaaaauu     2820

guccgcaagg ugcuuaguua uccacaagug aacaucguca aaaagacaga aguucagacg     2880

cauggguugg accggggaaa gcccagaggc cuguuuaaug cgaacccgag cccuaaaccc     2940

aagccagauu ccucugagaa uuuagugggu auuaaaagga accucgaccc uaagaaauac     3000

gggggcuaug ccggaauauc gaauagcuac gcugugcugg uaaaggcaau cauugagaaa     3060

ggcgucaaga agaaagaaac cauggugcuu gaguuccagg ggaucuccau ucuggaucga     3120

aucacuuuug aaaaggacaa acgcgccuuc cuacucggaa aggguuacaa agauauaaag     3180

aagaucauug agcugcccaa auauucauug uuugaacuga aggacggcag ucggagaaug     3240

uuagcuucua uccucagcac aaacaauaaa cguggggaga uucacaaggg aaacgagcug     3300

uucguuccac agaaauucac cacacuucug uaccacgcca agaggaucaa uaaccccaua     3360

aacaaggauc auauugaaua ugugaaaaag caccgcgacg auuuuaaaga guugcucaau     3420

uacguccugg aguucaacga aaaguaugug ggcgcaacua aaaaugguga gcggcuaaag     3480

gaagcgguug ccgacuuuga cuccaaguca aacgaggaaa ucugcaccag cuuccugggg     3540

gcuguaaauu cuaaaaacgc cggccuuuuu gagcucacgu cccugggaag ugcaagcgau     3600

uucgaauuuu ugggcgugaa gaucccuaga uacagggacu auaccccauc uucccuguua     3660

aaagauucaa uucucauaca ucaaaguauc acagggcugu acgagacucg aauugaccuu     3720

agcaagcugg gagaggauug a                                               3741


<210>  2904
<211>  3747
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2904
augaagaaaa uccugggccu cgaccuuggg agcgccucca uuggaugggc ugugauaacc       60

gagaacgucg aaaaugguau cacaaagugc gauauucugg gcaugggguc uagaaucauu      120

cccuacgagg gaacugaagg caaagacuuc acgaagggua ccggggaguc aaggaacagu      180

uugcggacaa aagcaaagac ugcccgcaag ggauaugauc gauaccaguu acguagaaaa      240

uaucugguug acacccuaau caagaaucaa auguacccug augaagcgac aaggaaccuc      300

ccaaaaauga agcuguggga gcuucgggac aaagcugugc acagcccggu aucccugaug      360

gaguugggcc gccuccugcu guggcucaau cagaagagag gcuauaaguc uucgagguca      420

gaugccaacu uagggaaaaa ggacaccgaa uacguggcaa cugucaaaag ccggcaugag      480

aagaugaaag aacuggaugu gacaauagga caguauuuuu accgcgagcu uagugccaau      540

gaguucuuua agguuaaaga aaacaucuuc ccccgagagg cuuacauuga agaguuugac      600

gcaaucugua agacccagca aauugcccac ccuaugaucu ugagauccga acugauagau      660

aagauuagga augaggugau cuauuaccag cggccacuaa aaucucagaa gggucucguc      720

agcgugugcg aguucgaagg cuuuuggcgu auugggaaag acggaaagga guauuucgua      780

ggccccaaag uugcuccuaa guccucacca cuguuucagc uugccaagau gugggaaaac      840

aucaauaaca ucaaaauugg uagucgcacg ggggaggauc ugaaguugac uaccgaacaa      900

aaaugggcga uauuccagca ucucgacaau aacgagaagc ugacacccac ugacuuacug      960

aaaauccuca aucuuaagaa ggagaacugu uacgugaaca aacagauggg aaagggccug     1020

aauggcaaca uuaccaaaca cgcaaucuug cagugccuag ggggaagcga uaaguaugaa     1080

aaucugcuca aacuggagcu uaagaugguc gacggugaua aggaaggcua ccuguaugac     1140

agaaaaacag gggagguguu gaacucuaag augauaaaau uuauugaucc uaugguggaa     1200

caagagccau ucuaccagcu cuggcauauc aucuacucca uucacgacca ggaggaaugu     1260

ucaagggucc ugcaagccaa guuugauauc gagcagccga uugcugacaa auuagccgca     1320

auagauuuca gcaagcacgc uuucggaagu aaaucugcca agguuauccg gaagauucug     1380

cccuaucuaa uggacggcga ugguuacucc gaagcgauga gcuaugccgg guacgaccau     1440

ucuaauucgc agaccaaaga ggagaaccuc cagcgaaagc ugcuugacuu gcugaaaccu     1500

aucccaaaga acucccuccg ccaacccauc guggaaaaga uucugaauca gaugguaaau     1560

guggucaacg caauaaucga gaaguauggc aauccugaug aaauuagagu ggagcuugcu     1620

agggaacuga agcagucaaa agaggagcgg aacgacgccg auaaggcuau gaguaaacgc     1680

cagagagaaa augacgccau cacgaagagg uuggaggaau uuggacuccg ugcaacucgg     1740

aacaauauua ucaaauggcg acuguacgag gaaauagaua accaagagaa gaaguuaaac     1800

gccaugugcg uuuauugugg ccagccaauc agccugacag aggcucuaag agggaaugac     1860

guggauauug aacacaucau ucccaaaucc aaacucuucg acgauucuca gagcaacaaa     1920

acccuggcgc auaggcacug caauaagaac aaaaaugacc uuacugcaua cgauuuuaug     1980

aagggaaagc cggaggccgu cuucaacgaa uacguggagc gcguaaauuu gcuguaugac     2040

aaccacauca uacggaaagc uaagagagau aaacuccuga ugacagaauc aaagauuccu     2100

gacaauuuua ucgacaggca guuacgcgag agucaauaca uugccaaaaa ggcacgagag     2160

auccugcaga ccauauguca ugauguuugg uccacgagcg guaccgugac agccgaacuu     2220

cggcacuugu ggggcuggga cgaugucacu augaaccucc agauggcuaa auaucgugag     2280

cuggggcuaa cccaucaggu ggaaugggag ucugagcacg gaaagaauaa gcaugaaaaa     2340

gaggucauca cagacuggac uaagagagau gaccacaggc accaugccgu ggaugcacug     2400

accauugcgu gcacaaaaca aggcuucauc cagcgcuuua acacccucuc agcugccaag     2460

acucgggaag acaugauguu cgauauugag cagagaaguc acgaauuuaa agagaagagg     2520

agccugcuug agaaguacau cauauccgaa caacccuuga aaguugacga gguggaaaag     2580

gcaguaucua auauucugau cucguucaaa agcgguaaga aagucgccgu gugggggacg     2640

cgaaagaucg gcaagcgcgg auccaaaaag guuauucaga aaaagaucau ugugccacgg     2700

ggcucauuaa gugaggaauc ugucuauggg aaaauaaaga agcaggagga gaaagauuac     2760

aaguaucucu uugaaaaccc ucagcugauc uucaauccca caauuaaagc uaagguggag     2820

gaaagacuuc ugauguuuga cggagaugug aaaaaggcca ucaccagcuu gaagaaaauu     2880

ccaaucuacc ucgacaacaa caagacugua guucuggaga aagcaauaug ucuaaaggac     2940

gaguucguca ucaaauacuc cguggauauu aauuucaaca agguggacaa agucguugau     3000

cccaagauca aggaaauucu gcaaaauagg cucaaaaagu uugguggcaa agagaaggag     3060

gcuuucaaag acgugcagcu ggaagauaag augaucauaa agugguauga agacgagggg     3120

cuugagcguc cuauuaaauc agugcgcugc uuuaccggau ugagcgccgu agucccagug     3180

aagaaagaug aaaacggcaa ugacaucggu uucguuaagc cggggaacaa ucaucacauc     3240

gcgauuuaca cagauaccga ggaaaacaaa cagcagcacg ugugcacuuu uuggcaugcc     3300

gucgagcgga agaaguauga cauacccgug aucauuaaaa auacaaacga aguuugggau     3360

aagauccaau cucagccuga cggcacguac ccagacuccu uccuggagau guuacccccu     3420

ccaaaucuca accugcuugu gaauaugcag cagaacgaga uguuuauucu gggaaccacu     3480

acagaagaua ucaaacuagc uauagacaag agagauuaua aaaucauugg cgacaagcuc     3540

uacaggcugc aaaagaugag uaucaaaccc agcucugggc agauugauau cguauuccga     3600

caccauuugg agacccagcu gauagacgau gaaaauucca agaaaucaaa gcgguuuuau     3660

aacguccaaa guuuaggagc acucuucgcc cugaacccgu uuaaagugcg cauugacaau     3720

cuuggcaacc uggugaagga gaaauga                                         3747


<210>  2905
<211>  3762
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2905
augaaggcca gaggcaacuu ccugcaccag gacacccccg ggcucacagc ugcaaaugcc       60

aacaugggag augcggugga gaaacuuugc gucgcucugg aagacuggug uacuuugagg      120

aauuuagagu gcaacauccg gaagaaucag aaaaacauuc aaaaucugcu agaagguaag      180

acgguugagg ugagcgccga aaacgcacuc aaagaguccu uugauucuuc acgcgccagu      240

cgaaaggaca aggcuaccau acugagcaca uggaucgcaa uucagccugg cgaguaccuu      300

gaugccaaac aggcuaagga cgccuccaaa gaaaucgcga auguacuguu cggguugcaa      360

ggaaacauga agaaaauuuu uaucgaguuc gaagaggauc agaagauacg ucucgacaau      420

gaugaacagg uggaagcauu uuauucucug uguccagacg auggcgucgc ccuguucgag      480

ucguuucagu caaucuacag cgcuuauauu cucaugggua ucuuaaagaa cgccaauggg      540

ggaaguauuu ccucuugcaa aguggcagcu uacgaccaau auaaggaaga ucugaaacuu      600

uugaaggacc ugguuagaga guucgccagc gaaaaauacg acgaguuuuu caggggcgau      660

aucuuugacu ccucaaaggg cuuccgggcg cgcaacuaua auccggccac ugcaaaaggg      720

uacaccaagu acaacagugu gagaggagcu agcuaugaug acuuuaagaa agaggucgaa      780

aagauauuca aagguacagu ggccgaggaa gauauuaggu acguagacau gaugcagcgg      840

uucgcugagg gcaccuuucu acgccgacuc aagacuucug auaacggguc cauccccuau      900

cagcugcauc uugaagagau ggacgccauu guugagaauc agaaacaauu cuacccuuuu      960

cuggaaucag agaaggaaaa guuggagagc cucgugacau ucagaaucag uuauuacguc     1020

ggaccacuga cccagaaaaa cgcaagguua gauaaucaca acaaugcccg guuugcuugg     1080

ucucagcgua aggaaggcaa agagcaugcg cgcguguauc ccuggaacug ggaggaagug     1140

aucgacagaa agcaauccgc agccaaauuc auggagaggc ugacggguac uuguaccuac     1200

auucucgaug aaccuguccu uccacggugc agccuguugu acgaggagua uugucuacug     1260

aaugaacuca acgggucgaa guggcgagac augggaggcg acuggcaccg cuuugauuac     1320

gagacaagaa uggaaauaau cgagaaucug uucaagaaaa agaaaggcuc uguuaaguau     1380

gcugccgugg aagacuggcu uaaaugcgag aagcauaagu ccugugcaga gguaaaaggg     1440

ggacagggug aaacuggcuu ugagucaaag cugaguagcu acguguucuu uaaagaaguc     1500

uugcacgugg augagauuga cgaagccgau auccccauga uagaggagcu cauuuggugg     1560

aacacccugu ucgaagacag guccaucuua aaggagucua ucgaagauaa auauggggac     1620

cggcugacag augaccagau uaaggcuauc ugcaaaaagc gcuuuaccgg auggggcaga     1680

cuaagcaagg agcuccugug ugguauuaaa acgcacacug auaaugggcc uuacucaaua     1740

auggacguuc uuagggaagg caacccaaau aacaaugccc guagugagac aaugguguuc     1800

auggagaucu ugcgggauga caaguucgga uuugacaaac ugauugaaga ggaaaacaug     1860

cgaagagucu ccgaggugga uaacuucgac guaaaugauc ucccgggcag ccccgcaguu     1920

aggcgcaccg ugaaccaggc gaagcggauc gucgacgaga ucguggggau ugcuggaugc     1980

gaaccucaaa auauauuuau cgaggucacu agagaugaag acgguaggaa caaaggccgc     2040

cgaacaaagc ggcguuauga uaauauuaag aacgccaucg cagcccugaa aaaggagcag     2100

aaagaauacu ucaauaagga cgugucugau ucacuuaaca aaaccaaucc aagugaccug     2160

gauaacgaga gauuggcucu cuacuuuaug cagaauggga agugcaugua uuguggaaag     2220

gaccuggaua uuaaacaguu agacaaguac caaguugacc auauccugcc ccagagcuau     2280

gugaaagaug acuccuucga gaacaaggcc cuaguacaca uggaagauaa ucagaggaaa     2340

ucugcaucga ugcuccugga cgauagcaua auccgcaaga ugaagccuua cugggaggcc     2400

cuucgggcug cgggcuugau uggugagaaa aaguuuaaaa accugacgag agucagggug     2460

uccgacaagg agcucggggg cuucaucgcc cgacagaugg uugaaaccuc acaaaguaug     2520

aaacugacau cuuuagugcu ggagaagacu uauccaaaua ccaacguccg ccccgugaaa     2580

gcuggauuua gcuccgaucu ucggaagaga uuggaccuca uuaagugcag ggagaucaac     2640

gauuaccauc acgcacacga cgccuaucug gcuugugaaa uaggccaguu cauuacaaug     2700

cgucauaaua aacuauacga gaacccgauc ucauacagcc acgccguaaa ggauuuuauu     2760

gcaaaacgcg cgaaguaugc caaugaccau gggacuccua ugucuaccua cggaaugccc     2820

gguuccagua gcuucgugau cucuuccuuu cugacaucag gcuucgacga agagaccggg     2880

gaaauauuca aagaugcuug gagugccgag guugcagcug uccggaucag agagagccuc     2940

aacaggaagg acugcuauau uucgcgaaug ccagaagaga cuuccggagc cuuuuggaag     3000

gaaacgcuga ucucuccucg ggaucccagc cuuggcaaaa auuugucacu gccaauuaag     3060

aaagguuuag acccccgcaa guacgggggc uauaacgagc agaaauucgc auacuuuuuc     3120

guguaugagg ccgugaagau caagaaaaag ggagaaguca aagaguacgu uuuugcugcg     3180

gugccugugc agguagccgc aauagucgcu agagauccaa augcccucac aggcuucgcc     3240

aagaaagacg cugaagcaag gaagauuaag uuucugcgga ucauccgaga gaaaguguac     3300

aaguaucaac uuauugaagu uaacggggag agacuguaca ucaccggauu gaaaagugug     3360

cgcaaugcca ggcagauugc gcucucccag gauaugacua ccgucuuccu gaaggcuaua     3420

aacgacaaag auguggccga ggacgaacua aagucucugu uugaggaaau cguucaacgu     3480

uaucggcucu gugcaaauag acugggcaac cagcuuaaga uuuucagcuu guccgauuca     3540

uuuaauagcg ccaacaguga gucuaaaaag agggcucugg uguccuuagu agcaaucauu     3600

gacgccaaaa caaauaagau cgaucucagc gguauagggu cgggaaaagc ggucggcaac     3660

augcgccugu cauucucuaa ggaaaugagu aacuccaaaa aucacuucgc uuuuaucgac     3720

cagagcguga ccgguauguu cgagcgaaag acuuaccuuu ga                        3762


<210>  2906
<211>  3777
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2906
augaccgagg aaaaccccga caucagaaag guguuccugg ccauucacca uauacucaaa       60

aauaggggcc acuuucuucu gcaggggcaa ucauucaagg agggaaacuu gaauaacuua      120

aucaaagaac ugcuaaaucu cgauauucug cauaucucca uugagaucac agaagagaac      180

auaaauaaga ucacugagau ugcucuugag aaaaagacgc ugaccgacaa ggucaacgau      240

uugaaaaaga ucuacccuaa agagaagcag cuccuggaag cauuucggcu gauuuucggu      300

ggcaaaacau cucucgacaa guuauuugcc aucgaugagu auaaggagcu ggacgcguca      360

guuaaaagug ugagcuucaa ggagaaaaua uacgaagaag uacgccacga uuaugaacag      420

gugcuuucca auuacauuga auugcuggac cuagcuaagc ucgucuauga uucuaucauu      480

cugucggaca ucaaaaagga ggggaaaacu cuuucagaga gcaaggugga acuguaccau      540

aagcaccgag aggauuugac caaacucaag acacugguua aaaacgacag uaaguuaucc      600

gaagauaaaa agguggagcu guauucugcc aucuuuaagg aagacaaaga caagggaacc      660

aauuacguca acuacauucg uaaaagcgag gagggcaagg guugcaauua ugaagauuuc      720

aaaaaguuuc ucgugaagga gcuugcaaaa cuggaagagu ccucagccaa ggaagagaua      780

uugagagacc uagagcugga acucuuccug ccacuucaga ggacuaaaga uaacagugua      840

guuccguacc aaauccacaa ggaggaacug gugaaaauuu uggacaaugc ugcaaaguau      900

cauaaguuuc ucaacgagaa agaugacagc ggguacucua ucauagagaa ggucauucag      960

cuguuagaau uucggauccc cuauuacgug ggaccucuga acuccagcaa aaaggccaaa     1020

guggauggcu uugcuuggag cguccgcaau aagggcuaug agaacacacc aguuaccccc     1080

uggaauuacg aaaaagugau cgacgagagu gccucugcgg aaaaguucau uacgaaccua     1140

acuaauaagu guaccuaccu caaaggggag gauguacugc cuaaguccag ccuuuuguau     1200

ucggaguucg cacugcucaa cgaacugaau gcccuuaaau acgacggaaa cagaaucucu     1260

cuggaggcua ggaaugugau uauagaaaag uuguuuaaag agcaggguaa gaaggucaca     1320

aaaacuucca ucaagaaccu ccugaaagcc gaaggcuaua uugaugggaa gggagagauc     1380

accggcaucg acauuacagu gaaaaaugac uuaaagucau accgggauuu caagaaaaua     1440

cugggugaca aguuuaacac cgagcacguu gaaaauauca uucuauggau cacgcucuau     1500

ggggagaguc gcaaacugau uaaggcaaaa aucgaagcug uguacggaga uauauauagc     1560

aaggaggaaa ucgccaagau gucccgacuu gucuacaaag acuggggcag auucucuagg     1620

aaguugcuga cugagcucgu gagcaaaaag cuguacaacg aggaaacagg cgagugcuua     1680

aauauuaucg gggcgaugcg gcagaacaac auucuguuua uggaacuuuu ggccgaucgu     1740

uucgacuauu cacaacagau cauagauuac aauaaagagc uccagaagga gguaaccgaa     1800

auuacuccag agauccugga cgaucuauau guuagucccu ccgugaaacg cagcauuugg     1860

cagacaguca gaaucgugga ggaacugaag aagauaaucg gaugugcucc ugcaaaaauu     1920

uuugucgaaa ccacgagguc uaaccaagac aagaaaaagc caaccgauuc acggaaaaag     1980

cagcucgagc uguucuacaa ggccgugaaa aaggacguua aagaucuuga gaaggaaauc     2040

gguaguuuga auuuugacaa acugaacgag cgauuaagcu ccguggaacc gucuaagcuc     2100

aaggcuaaaa agcuguaucu uuacuauaca cagcugggcc gcugcaugua cucgggggag     2160

acuauugaca ucacccaauu gaauacaacu gccuacgaua uagaccauau uuauccccag     2220

agcaaaguaa aggaugacuc cuucaccaac acaguccucg ugaaaagaga auacaaugca     2280

gccaagaccg auagguaucc ucugggauca gacauccaga cuccaaguaa ccggcgcuuu     2340

uggaaguucc uaaaagagaa gggccugauc acggaugaga aauacaauag acucguuagg     2400

acagaagagu uuucugacga ggaacugacc gguuucauug cucgucagcu uguggaaacu     2460

agccaauccg ucaaggcggu ggcagggauc uugggcgagc ugaaccccaa aaccucaauu     2520

uguuauagca aggccgagaa uguaucuucc uuccggcaga acuuuggaaa gauaaaagaa     2580

ggcaaucgaa agagugauaa cacagagcag uuaaucaaag ugagagaaau uaaugacuac     2640

caccacgcua aggaugccua ucucaacguu gucgugggga acguguacga caccaaauuc     2700

acuaggaaug ucuuuaacuu caucaaggau aaaaaggacg gacgcaagua cagccugaau     2760

aacguuuucu augagaaugu gucuaacucc aauaacgugg caugggagcu uaauaaaaca     2820

guacaucagg ucgaaaaggu gaugaacaau aacaauauuc ugguuacgcg gagaaccuca     2880

gagcaaaaag guggccuauu cgacgccacu guguacaagg cuaaagucgc cgcaaaggcg     2940

aaggaagggg uguauuaccc ucucaaaaca agugauccag uugugaacga cguaaccaag     3000

uauggaggcu uuacaaaagu caagaucgcu uacuauagca uauucgagua cacucuggug     3060

aauaaaaagg gugaagagaa gaucaccagg auuaucccca uuccgaucua cgugucgcag     3120

aacauaaaag augacuccac guugcuggag uuugggaaga cccagauuaa caucaaaucu     3180

ggcgaagagg ucaaggauuu caaauuaaag uaucgcaagc ucugcaucgg agaccugguu     3240

aaaauuaaua acuuuuucaa uuuuauaggc gggaagacaa acgauagcuu cguguacgac     3300

ggagccguac aggugacucu ugauaccgaa ucagagcgau auaucaaaac acugaguaag     3360

uaccaaaauu ggaaaaagga aaacaaagac ggugaguugu gggaguccau uacuaaggaa     3420

aagaauaccg agcucuauaa ccugcuaguc gagaaaauga acucuggcau cuucauuaag     3480

aaaaagccua auaaauuuaa ugaacugaac agcgaagaga uccggaauaa cuucuccuca     3540

auaagccuug aggaacaggc aaaggugcuc cuggagguuu ugaaucuguu aacgaacaag     3600

aaaaguaucu uugaucucaa gucuauuaau aucacagccg cucgugggaa aauuuccuuc     3660

aaccugagca accuuucgca guuuucaauc auagaacagu cuguggccgg auucuacgag     3720

aaggagauua ccaucaucgg cgacaaagaa aaugacaugg agaacaauaa cuguuga        3777


<210>  2907
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2907
augaaguaca uccugggccu cgaccuuggg guggccagca uuggaugggc ugucaccgag       60

auaaacgaaa aaggugagcc cguuggccug aucgaugcaa auuccgugau uuucaagccu      120

uuggacaacg auaaagggaa guuauauaau guagaaagaa gggacaaacg gggaucucgc      180

cgaauccugc guagaaagaa gcacagggug gagcggacaa aaaugcuacu cgucaacuca      240

aguuuucuga cuaaugaaga gauugauaac cuuuacgugg gcaagcugga caauaucuau      300

gagguucgcu ugaaaggucu caaggaacag cugagcaaaa acgagauagc cagacugaug      360

aucuacuauu gcaagaauag gggguucaag uccaaccgga aaacggaaga ucucgagauu      420

uuaaagucuc ugggagagaa aaucucggaa aaggacucag augagaaaaa gcugaaacca      480

auuaucgcga agaauaccga auugauagag agcaagggcc ugacaccgau ugaagugauc      540

uacaugauuc gcgagaacga caauaguauc cuaggcuuua aaaacaagga ggggaauuau      600

aaauucggau uuaagcgaaa ccaagucauc gaugaaguga gaaaaauucu cgguacucag      660

cagauacugu ccaaggagau cguagacgaa uacauugaua uccuuucuag ccagagggac      720

uucuccgacg gccccggggg agauucaaag uacaaaauag acuauaccaa gcuggcuggc      780

aaauguaagu acacagguga gguucgggcc gugaaaagug caccuagcua ugaaauuuuu      840

accauguugc aaaagcucaa cgauauccgu uacgucaagu ucacuucuga ggacaaaauc      900

gagaagaaaa agcuguccaa agaagugauu cauaaguuau augaucuggu ggucgagaag      960

aauaaaacac ucaccuacga ccuuaucgaa aagucaauug augaggaaaa cauaaaacug     1020

uugaauaucc caaagcuaag caaaaguaag uauguugagc ugcgcaaauc uuacuuuaag     1080

gagagaaacg acaaugaaaa ccucacggag gaagagauua augccuucaa cgagaagcug     1140

aauaacgaaa ggaugaaaca ggagcuuauc cggaauaacc ugaaggcuua caaagaauug     1200

aaguccaaau uuaagaagaa uaacaucgau gagaaucgaa uuaaagaacu cgggggaaua     1260

uauuuccugg acuuaguggc agagcugcua acuuacagca agaccgauga gaaacucgaa     1320

uauuuuguag agaacgugga acgcuacucg cuguucaagg agcagagaga caucgucgaa     1380

auuaucaaaa cauuccccaa cuauacuaag aauggcaacc uuucuuuguc ccuggugagg     1440

gagcucaaua agcugcuuau ggagggccac gacuacgaau caagucugag cuccuugaac     1500

uauuacauug auaccgacgu ugauugggag aaauuuccua caaucucuga aauagaggac     1560

agccucucaa ccaagaucac gaauccaaac gugaaacaua uucuggucau cuuacggaag     1620

cuguacaaua cucuacucuu caaauauggg cgccccgaaa aggugcaccu ggagcuuagu     1680

agagauuuuu ccaacgacuu cagcacaagg aauaagauuc agaaagagca auuggaaaac     1740

aagguacguc gggagguugc cgcuuuugaa auguacggag ccaauaaaga uaucguggcg     1800

gguaaggacc gacugucuaa cgaugacuuc gucagaauaa aacucuggga ggagcagaau     1860

aaggugugca uguauucagg caggaccauu gaaaaauacc agcugacaag ugcagagguc     1920

cagaucgauc auauuuuacc uuauagcaag uccuuugaca acucuuacuc gaauaaggug     1980

cugguuuuca gcaacgaaaa ccaagauaaa aaggagcgca cuccauauca guggcuuaaa     2040

gggaccgaaa aguggaauga guuuaaacag cgggugagau ugaaccucaa uaucuccaac     2100

aagaagaaag agaaucugcu auucgaagac gagguaguca acaaugaauu ucuggagagg     2160

gaacuccacg ccacgucaua caguucucgc cuggcucuua acauauucca acgauugauc     2220

ccggugagcg acgaggauaa guacgacgag cacggaaaug aaaaaguuaa guaucuguac     2280

aaccggaaug ugauugccuu ucagggcaaa augaccucca uguuacguaa ccucuauuca     2340

cugaauaagu acacacauag cuucgagucu gauacucuug acaucgauaa caaggcauuc     2400

auucugaaag aauuugagau caauaaggac aacuugaaaa ugaccgcuua uaacgucaau     2460

acaggucucg aaauaacuuc cgagaccaag guggagaaaa acaagagugg ggaguucaag     2520

acaauuaaag augaacugcu aaagaaagaa cuggacaaga aagagaaucu caucgagauc     2580

agcgauuacu uuaaggacaa gguacuguuc gaucuuaaaa uuguggacuu ugaagagauc     2640

gauaccguug aacccgagau uauaucuauc uuguauaaga ugauuacugc ccugaaagaa     2700

gaggucuacu ccaagaacag agagaaucac uuacaucacu cacucgacgc guuccugcuu     2760

acgaucauga acaggaguau gcagaugaaa cugauuaagu acaaucagcu aaucagcgug     2820

cucaaaaaca aggaaauaga gaucuuuaau gaagagaagg gcgaauauau ugacucgaaa     2880

gaguucgccg aggaacugca aaagcagaaa aucauugaua ucgacggaaa cgagcgcggc     2940

auaaaguuua augugaacgg gaaaacacac cgguugaauc uggucaagcc uuacgaaaac     3000

uucuuagaug acauuaagaa uaaaaucuuu gauaagagag agaacaauaa acucccauau     3060

cauguuguga aguccaaagu gucuggagca cugcacgcug aaaccauccu uggugagagc     3120

aagggcgaga uuacuaagag gauaucagua uucgacauca acuacaaaaa ccuggggaag     3180

auuuuugaua aagacggaag ucagaaggaa aucuaugaga ccuuggucaa auggcucgaa     3240

gccaagucca aggauuaccc caaacugaag aauggcaaca uuaucaaaaa ggugaaaaua     3300

guugacggua auaaggauaa acuaaucaag cuggggaaca agcgauaugu ggagaugggc     3360

cgcacaaccg ucaaaauucu ugugcucaag aaagaggcug aagagggacu gaaguucgcc     3420

ucuaucggcc gguacaaaua caauuugcuu aagagcgaaa aggacguuaa cauuuccauc     3480

uggaaagacg caauacauuu cuguaaggug agauauaaua aauuaaagga gaacggguac     3540

aggcucauuc acgaacugau cccuggagag acuaucgagc uugaacugaa uaaagguaac     3600

auuucaaagu gcuugguagu cggcuuuagc gggggaaaga ucgagauaag uucugugauu     3660

ggcgaugccc ucgaccugau caaugauaaa auuuccacac guaucaagga acgcuauuac     3720

auaacgguga gcaccauuaa aucgaucaag aaaaucaaca agaauauucu aggugacuga     3780


<210>  2908
<211>  3792
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2908
augcacaaga acaaucccga caucagagag cuguuccucg ccauucauca cauacuuaaa       60

uacaggggcc auuuucuguu cgcugggcag caauuuaagg gaagcgaaau caaagauaac      120

auucgggagu uguuacagua uaauucccug aacaucaaga uugacaucac cgaugaacua      180

auaaauaaca ucgcagagau ucuccugaaa aagaaaaauu cuacagacaa aaucauugag      240

uucaagaaac uuuuugccga ugagaagcag cugaucgagg cguugcgccu cgcucugggu      300

aacaaaacuc agcuggccaa acucuucgac auagaagcau acaaggaguu agauauuuca      360

aucaaaacga uuaguuuuaa ggagaaaauc uaugaggacg ugcgaagcaa uuacgaggaa      420

auccugggcg aguauauuau aguccuugau accgccaagu ugaucuacga cgcuauuauc      480

cuguccgaaa uaaaaauuaa gaacaugaca aucucugagu cgaaaguuau cucauaugaa      540

aagcacggga aggaucuaaa acaacucaag cagcugauua aagaggacag caaacuuagu      600

aaaaaggaga agcgucagcu guucgaaaau aucuuuguga aagaggauga aaaggaggca      660

aacuacguaa auuacauugg aaagaacaau gugggcuccu cuaagagcuc cugcucauau      720

gaagacuuca aaaagaguuu gauaaaggag cucaaauacu gugaggaaag caagcugaaa      780

caggacaucu uagccgagcu ggaacucgag acuuuucuuc cuagacaaag gaccaaggau      840

aacucuguca uuccauauca gauccauaaa gaagaacugg agaagaucuu gaacaaugcu      900

gugcaguacc acccguuccu aaacaauaca gacgaauaug guauuuccau aaaggagaaa      960

aucauucagc ugcucgaauu ucggaucccc uacuauguug ggucacugaa caccagccac     1020

aaggugggaa auggcggcuu caguuggucu guccgcaaca aaggguacga gagaacugcc     1080

gugacaccuu ggaauuucaa caagguaauu aauaaagagg aauccgcgaa gaacuuuauc     1140

gagaaucuua ccaaccaaug cacguaccug uuguaugaag auguucuccc aaaacauagc     1200

cuguuauacu cggaguucac ucugcuaaau gaacucaacu cuauaaauua uggagguaag     1260

aggcugaccg uggagcaccg gaaccuuauc auugagcgcu uuuucaagca guccgcaccc     1320

aaagccacag gcaaggaauu gaaaaaguuu cugaugucag agggggucau cgacggaaaa     1380

gaugaacuca guggcuucga cauuagcauc aagaacgauc ugaaguccua ccgagacuuu     1440

aaaaagauac uuggugagaa auucgauucu gaaaaggugg agaaaaugau uagauggauc     1500

acucuguuug gggagagcaa gcaguugcuc guggacaaga uuaccaaaga auauggagau     1560

aucuacucaa gugaggaaau aaagaaaauc uccggccuga cauauaagga cuggggcagg     1620

uuaagcgaga aacugcuaac cgaaauuguc ucuucacaac ucuacgacga gaguacgggg     1680

gaguguuuca auaucauuaa cgcuaugcgg aauaacacuc ugcuuuuuau cgaauugcug     1740

ggaacaaauu acgauuauuu cgagaagaua aacaccuaca augguaagcu ccguaaagaa     1800

auuacugaga ucacaccuca uauccuggac gauuuauaug uuagcccagc cgugaagcgc     1860

uccauuuggc agguagugag aaucgucgag gaacugaaaa aggugauugg cucugagccc     1920

aaaaggauau uuguugaaac cacgcggucg aacaaggaga aaaaggggga cgauggacga     1980

aaggacagcc gcaaaaaggc acuuuuggaa cucuacaaaa agaucaaaac cuccgauauu     2040

gcugagcugg agaaggcccu aggccacaca guggacuucg auaagaaaaa gcaggaccug     2100

acugauaucg acaauaacaa acucaaggcg agaaaacugu aucuuuacua uacccaguug     2160

gguaggugcc uguacacagg gaaggccauu gauuuaaaua accucuucgg cucaaauagu     2220

gaauacgaca ucgaccacau auauccucaa ucuaagacua aagauggaag cuggaacaau     2280

cugguccuug ugaacaagac cgagaacucc aaaaagucaa auggccugau cagcgaaucu     2340

auucagcgga aaaacaagca uuuuuggcgc uucuugaagg cuaaaaaucu caucuccgag     2400

gaaaaguaca aaagacugac aaggaccgag gacuuuacug auggggagcu aaguggauuc     2460

auugcacguc agcugaucga aacgagccag ucuguaaagg uuguguccaa aauacuccaa     2520

aaccugaauc caacaaccga gcuuuguuuu ucaaaggccg aaaacgucag ugacuuccgg     2580

caggaguuug guaagauuaa agugggcaag cgaaagaauu ugacugaaga ggagcagaag     2640

aaagcuaagg aagggcagcu gaucaaaguc agagauuuaa acgaccucca ccaugccaag     2700

gaugcauauc ugaauaucgu gguuggaaac guguaccaca uuaaguucac caggaauccg     2760

aaaaacuuua uacgcaagaa uuucaaaaac cgggaguaua cacuuaauac ccuguuuuac     2820

agcgacaagg uagaaagaaa cggcgagguc gccugggaga aagguaagac ugaagagauc     2880

gugucguccg uuaugugcaa caaucuaauu aacgugacaa ggcgcacguc ugaacaaaag     2940

ggggcucucu augaggcgac caucuacaaa gcaaaugccg cuaaggaagg cagcuacuau     3000

cccgucaaaa cuaacaauaa gaaccugaaa aaugugacac gauacggagg cuucaccuca     3060

auuaagaucg ccuauuacag uauauuuaag uaugauaucc ggaacaaaga caagguaaaa     3120

gagaauauua agaucauucc uaucccaaua uacauuuccc agaacaucaa aacagauuug     3180

gaccugauca auuucgcaga gaagcagauu aaguuaaaga agggggaaaa caucgagaau     3240

cuccagcuga uauauaaaaa gcugaacauu ggagcccuug ugaaaaucga ucauuacccc     3300

uacuauauug guggcaagac ugacgacaau uucuacguug auggggcugu ccaaguguug     3360

cucucuagcg acaucgaacg uuaucugaaa gaggugguca aguuuguuaa cuuccagaga     3420

gaaaauaagg aggccaaagu guccaaggag cacauaucac uagaagagaa cauuaaacug     3480

uacaacaccc uuguguguaa gauggcaacg aaaaucuuua cccacaagaa gaauaacaaa     3540

uauaaugauc ucacacagga caaggcgaaa aacacuuuca auaagcugag cuuggaagaa     3600

caaugcaaag uacuguuaga aauccucaac cugcuuacca auaagaagac aacuuuugau     3660

cugaguuuga uuaacgagaa aaccucuagg gucaagugug gauucaaucu ccugaaccag     3720

aaacaguuuu ccaucauuga gcagagcgug acgggccuau acgaaaagga gauaaaaauc     3780

auugaagacu ga                                                         3792


<210>  2909
<211>  3795
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2909
augaagaacg ugcugggccu cgaccuuggg accaauagca ucggaugggc ccugauugag       60

gucgaugaaa acaauuugcc cauaagaauc auugcuaugg guuccaggau cauuccuuua      120

ucuacaucag acaaagagga guuccagcgg ggccugagua ucacuaagaa ccaaagccgc      180

acgguuuccc gaacccagcg uaaaggguac gauagaaagc agcuaaagaa gucugaccuc      240

aagaaaauac ugaauaagua uaacaucuuu ccaucggagg aacuucugag guugccgaug      300

cucgaucugu ggaaacugcg gucagacagc gcaaaucccg augagaacau uagugccgag      360

cagcucggac gcaucuuaua caugcugaau caaaagagag gcuauaaauc cgcgaggucu      420

gaagcuaacg ccgacaagaa ggauagcgac uacgugcagg caguaaaagg ucgguaugau      480

cagcuuaagg accgcaaaca gacaauuggg caauacuucu augaugaguu guacaaugcc      540

aacgcuaaua acuccuacuu ucgaaucaag gaagaggugu auccuagaga ggcauacaua      600

gaagaguucg acacuauuau cucagcccag agggaaaaac acgacuuucu gaccgaugag      660

gucgugcauc agcuacggaa ugaaauuauc uauuuccagc guaagcucaa aagucaaaag      720

ggacugguua gcaucugcga guuugagggc uucgaaaaga caguguuuga caaagagaag      780

aacaaaaaca aggucguguu cguaggccca aaaguugcuc ccaagaccuc uccucuuuuu      840

cagcuguguc gcauuuggga aguggucaau acuauaacau ugaagaucaa aaacccagag      900

ggguccaagu acaaaugggg agaaaagauu cccgauauca aagagaagga gauacucgcc      960

gacuaucugu ucaagaauga aucacuuagc uuuaguaaac ugcucgagau ucuugaacug     1020

aagaaagaga acguguacgu gaauaagcag aucuugaaag guaucaaggg caacgaaacc     1080

uauucugcgc uacacuccau ucugggggau aaugagcucc ugaacuucga cgucagcauc     1140

augacgucga aucaaacugc acuucugguu gauagaaaga ccggagagau uuuggaagag     1200

agggccggcc ucgaacugga cgcuucuuua gagaaagagc cucuguacca gcuauggcau     1260

acaauauacu cccucaagga ucuggacgaa ugcaaaaacg cccuuaucaa gcgguucgau     1320

uuugacgacg agacugcaga aaaauuguca cgaauugauu ucaauaagca ggcuuuuggu     1380

aacaaaagua auaagagcau gcgcaagauc cugccauucc ucauggaggg guaugacuac     1440

ucccagucua gcucacuggc cggauauaac cacaguaauu cccuuaccaa aaacgaaaga     1500

gagcaacaga ucacacugga uagguuggag cuccugccga agaacagcuu acggcagccc     1560

auuguggaga aaauacugaa ucagaugauc aacguaguga augcgauucu agacgaauac     1620

ggcaagccuu cugaaauccg cgucgagcuc gccagagaac ugaaacaauc aaaggaugag     1680

aggaacgaga ccgaccuuca gaauaguaag aacaaaaagu ugaaugauga aauuggcaaa     1740

cgucuggagg aacucgggcu gccagugacg aagcgguaua uccagaaaua caaguuuaua     1800

uuccccagcu uauccaagaa ccugaaagag gcaaagguua auaaccagug uaucuauugc     1860

ggagaaacuu uuaaucuuac agaggcuuug ucuggugacg ccuacgaugu ggaccauauu     1920

gucccuaaag cucuccuguu cgaugacucg caaaccaaca aggugcuagu acaccgaaaa     1980

uguaauagca cuaagacaaa ccagaccgcc uacgauuaua ucgcaacgaa gggcgacgag     2040

caccugaaac ucuacuccga aagacuggac gauugguuug agagggggau uaucucauau     2100

aguaagaugc agcgccuuaa aguuucucau aaggaauacu uggagcggaa aaagcuggga     2160

aaagagaccg aaacagacaa gaaguuaugg gagaauuuca uagauagaca gcucagggac     2220

acucaauaua uuagccgcaa aucccgagaa auccugcaga agauuugcaa cgaugugaaa     2280

accacagagg gcggugucac ugccaaacuu cggaaccugu ggggguggga cgauguguug     2340

augaaucucc agaugccaaa auacaaggaa cugggccaaa ccaucacaaa ggaguggacc     2400

ucagagcacg gaaaacguaa gcaucagaaa gaagagauag acaacuggac uaagagagau     2460

gaccacaggc accaugcuau cgaugcgcua gucauugcau guacgaaaca gggcuuuauc     2520

cagcgcauua auacacugaa cagcucugac accaaggauc ucaugaucaa ggaaguggag     2580

gaagccaaaa uagaguucaa ugagaagacu acccugcuug aaaaauauuu gaagacacaa     2640

aaacccuuuu ccacccagga gguuaugaag gaagcugaca agauucuggu gaguuucaaa     2700

gccgggaaga aaguagcaac uaucacaaag uucaaagcca cgggaaagaa caaggagacc     2760

ggugucaucg ugccgcgggg cgcuuuacac gaacagagcg uuuacgggaa aauuaaggug     2820

aucgagaaag acaagccucu caaauaccug cuugaaaauu cugauaagau ugucaacccc     2880

agaaucaaaa aucugauaga aauuaagcua uccgagaaca agaaugaccc uaaaaaggcc     2940

cuccuggcau ugaaaaagau cccaauuuuu cuggaugagg aacagucaau cacuuuagag     3000

aaagcgaguu gcuauaacga cgcuacagug cucaaguaca agcugcaaaa ucuuaaagcc     3060

gcacagguag augacauagu ggaugaaaag guuaaaaaga ucgucaggga gcgacugagc     3120

caguucggaa acaaagaaaa ggaggccuuu aaggacaccu ugugguucaa ugaagagaaa     3180

aagauuccca ucauuacagu gcgccucuuu gcucggccag augccaacaa ccugcagacu     3240

aucaaaaagg acgagaaugg caacgguaua ggguucgugg ucaccggcaa uaaccaucac     3300

auugcaaucu augaagauaa agacaauaag cccguucaac auucguguac guuuuggcac     3360

gcuguggaga gaaagaaaua caacaucccu gccgugauua gggacuccuc ugaaguaugg     3420

aaugaucuac ugcguaagga gcuuccagag agcuuccuca acaaacugcc ggcggacaau     3480

uugaaccuga aguuuucaau gcagcagaau gaaauguuca uauuaggacu cagucaagau     3540

gaguuuguca aagccaucgc uaacaaugac aagucccugc uuucuaaaca ccuguauuug     3600

guguggagca uuuccgaaaa caauuacugg uuccgccauc accucgagac caagaacuca     3660

gaucugaaga aaacagacgg cguuaaggaa agcaaacggc uauauaauau cagaagucug     3720

gggucuuuau uuucccucaa ccccauuaag gugaggcuga acuacuuggg agagaucacu     3780

aaaauaggug aauga                                                      3795


<210>  2910
<211>  3801
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2910
auggccaagu acauccuggg ccucgaccuu gggaccaaca gcauuggaug ggcucugaua       60

gagguggaug aaaauaacau ccccauuaga aucauuguca uggguuccag gaucauaccu      120

uugucuucaa augaccggga ggaguuccag cgcggcuuaa guaucacuaa aaaccaaaau      180

cgaacaauua gccguacgca gagaaagggg uaugauagga aacagcugaa gcggaacgac      240

cuaaauaaag uucucaacaa guacaauauc uuuccaucca agaugcugcu uaaccugccg      300

auguuggauc ucuggaaacu gcgcucugac ucggcaaauu caaacaagga uauuagcgcc      360

gaacagcugg gaagaauccu cuauauguua aaucagaaaa ggggcuacaa gagugugcgg      420

uccgaagcga acucugacaa aaaggauacc gacuauguac aggcugugaa gggucgcuac      480

gcccagcuga aagaucgaaa ucagacaaua gggcaauauu ucuacgagga gcuuuacaac      540

gcaaacguca auaacagcua uuuuaagauu aaagaagagg uguaccccag agaagccuau      600

aucgaggagu ucgacacuau uaucaauucc cagaagacca aacacgaauu uuugacagau      660

caguucguuc aucagcugag gaacgagauc auuuacuuuc aacggaagcu aaaaucacag      720

aagggacuca uaaguaucug cgaguucgaa ggcuuugaaa agaccguguu cgacaaagag      780

aagaauaaac acaagauugu cuuugugggc ccuaaaguag cuccacguag cgcaccccug      840

uuccagcuuu gucgcguuug ggaggugauc aacacuauau cuauuaaggu caagaauccu      900

gaagggucca aauauaagug gggagagaga aucccaucaa ucaaagaaaa ggagauucug      960

gccgacuacu uguuuaaaaa cgaagugcuc agcuucacaa agcuguuaga gauccugggu     1020

cucaauaaga acgaugugua ugucaauaaa caaauucuua agggcauaaa agggaacgag     1080

accuacgcua cgcuguacaa uaucuuggga gaaaacgagu uccuaaauuu ugacguuagu     1140

auuauccccu cuaaccauac uuccaugcug guggauaaga aaaccggcga aauccucgag     1200

gaaagggccg gucuggagcu ugacguaagc cuggagaagc agccuuucua ucaguugugg     1260

cacacaauuu acucgcucaa ggaucuggac gaaugcaaaa acgcguuaau aaagcgguuu     1320

gauuucgacg augagacugc agaaaaucug ucucgaaucg acuuuaacaa acaggccuuc     1380

gggaauaagu ccaacaaauc aaugcgcaag auucuaccau uucucaugga gggauaugau     1440

uacagucaaa gcuguucccu ggcuggcuau aaucauucua acagccuuac caaagacgag     1500

agagaacaac aggugacauu ggacaggcug gagcuccugc cgaaaaauuc acuucggcag     1560

cccaucgucg aaaagauucu gaaccaaaug aucaauuugg ugaacgccau acucgaucag     1620

uacggcgagc ugagugaaau ccgcguugag uuagcaagag agcugaaaca guccaaggac     1680

gaaaggaaug augcugaccu acagaacacc aaaaauaaga aacucaacga ugagauuggg     1740

aagcgucugg ccgaacuugg auugccuauc acgaagcggu auauucaaaa auacaaguuc     1800

aucuuuccaa gccugucuaa aaaucucaag gacgcgcacg ugaacaacca gugcauauac     1860

uguggugaga cuuucaaucu gucagaagcc uuaaguggcg augcauauga cgucgaucac     1920

auugugccca aagcucugcu uuuugacgau agccagacaa acaagguauu gguucaucga     1980

aagugcaauu ccaccaaaac uaaccagaca gccuacgacu auaucucuac caagggggau     2040

gagcaaguga gaauuuacuu cgagaggguc gacgacuggu uuaaaucggg aaucauaagc     2100

uauuccaaga ugcagcgccu caaaguguca cacaaggaau accuggagcg gaagaaaauc     2160

ggcaaggaaa cggagaccga uaaaaagcua ugggaaaauu ucauugacag acagcugagg     2220

gauacacaau auaucagucg caaaucucga gagauucucc agaaggucug uaacaaugug     2280

acuaccacag aggguggggu uacugcuaaa cugcggaacc uuuggggcug ggacgaugug     2340

uugaugaauc ugcagaugcc uaaguaccgu gaauuaggac agaccguaau caaggagugg     2400

acaagcgaac auggcaaaag aaagcaccaa aaagaggagg ucgaaaacug gaccaagagg     2460

gacgaucacc gccaucacgc cauagacgca cucgugauug ccugcacuaa acagggguuc     2520

auccagcgga ucaauacgcu gaacuccuca gauacaaagg accuuaugcu gaaggagguu     2580

gaagaggcua aagugaaguu uagcgagaaa accacuuugc ucgaaaagua ccugagaacc     2640

caggagccau ucucuacaca agaagucaug agggaggcgg auaaagugcu aguauccuuu     2700

aaggcaggaa agaaaguggc caccauuacu aaguucaaag cuaucgguaa gaauaaagac     2760

aguggcguua uugucccccg aggggcccug caugaacaga gcguguaugg aaagauaaag     2820

gugaucgaga aagauaagcc gcucaaauac cuguuugaaa acucugaccg cauugucgac     2880

ccuaagguua aaaaguuggu ggaggugcgg uuguccgaga augaaaacaa cucaaagaca     2940

gcaaucagua cgcugaaaaa ggcccccauu uucuuaaaug aggagaaaac cgaaguacuc     3000

gaaaaggcua gcuguuauaa cgaugccacu guccugaaau acaaacuuca gucgaucaaa     3060

gcaucccagg cggacgauau aguggacgag agaguuaagu uucuggugaa ggagaggcua     3120

gcucaauucg gcaauaaaga aaaggaggcc uuuaaagaua uccucugguu caacgaagag     3180

aagcagauuc caaucauuuc uguccgucug uuugcacgcc cugacgccaa uaaccuucag     3240

gugaucaaaa aggaugagaa ugguaagagc auaggguucg uucugacagg caacaaucac     3300

cauauugcua ucuaugaaga caaagauaac aaguuaaucc aacacucaug caccuuuugg     3360

cacgccgugg agcggaaaaa guacaauauu cccguaguca ucaaaaacag uuccgacgug     3420

uggucucagc uccuggauaa gcagcuucca gaaagccugu ugaauaagcu ccccacagac     3480

aaacugaacc uaaaguucuc caugcagcaa aaugagaugu ucauacuggg acuuucacag     3540

aaagaauuug acgaggcaau uaagaacaau cauaagagcu uccucaguaa guaucuguac     3600

uugguguggu cuaucuccaa caacgauuac uauuuuagac accaucugga gacuaagaau     3660

accgaauuaa agaacauuag cacggcuaag gagucgaaaa gguacuuccg auuuaaguca     3720

gucggcgccc ucguugggac caauccuauc aaaauacggc ugaaccaccu uggagaaauc     3780

aauaaggugg gugaguauug a                                               3801


<210>  2911
<211>  3807
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2911
augaccaaga acauccuggg ccucgaccuu gggacaaaua gcauuggaug ggcccugaua       60

gagguggaug aaaacaauac ucccaucaga auuaucgcua uggguuccag gauuaucccu      120

uugucuacgu cagacaaaga ggaguuccag cggggcuuaa guauaaccaa gaaccaaagc      180

cgcacagucu cccgaacuca gcguaaaggg uacgauagaa agcagcugaa aaagucugac      240

cuaaagaaaa uccucaauaa guauaacauu uuuccaucgg aggaacugcu uaaacugccg      300

auguuggauc ucuggaagcu gaggucagac agcgcaaauc ccaacgagaa uaucagugcc      360

gagcagcugg gacggauucu cuacauguua aaccaaaaac gcggcuauaa guccgcgaga      420

ucugaagcua augccgauaa gaaagacacc gauuacguuc aggcagugaa ggguagguau      480

gcccagcuga aagagcggaa ccagacaauc gggcaauacu ucuaugagga acuuuacaau      540

gcuaacacca auaacagcua cuuucgcaua aaggaggaag uauauccucg agaggcauac      600

acugaagagu ucgacacaau uaucaacgcc cagaaaaaua agcacgacuu uuugaccgau      660

gaaguggucc aucagcugag aaacgagauu aucuauuucc agaggaaacu aaagucccaa      720

aagggacucg ugucaaucug cgaguuugaa ggcuucgaga aaacgguuuu ugauaaggaa      780

aagaauaagg acaaaguggu cuucgugggc ccaaagguag cucccaagac uucaccucug      840

uuucagcuuu gucggauuug ggagguugug aacaccauaa gccugaaaau caagaaucca      900

gaagggucua aauacaagug gggagagaaa auucccaacu ugaaggagaa gcagauccuc      960

gccgacuauc uguucaaaaa ugauuccuua ucauuuacaa agcugcucga aauacuugac     1020

cugaacaaaa augaugucua cgugaacaag caaauuuuga aagguaucaa gggcaaugag     1080

acuuucagca cccuacacac agugcugggg gacaacgaac uccugaauuu cgaugucagu     1140

aucauuccuu cuaaccauac cuccaugcuu guugacaaga aaacgggaga gauccuggag     1200

gaacgugcgg gcuuggagcu cgaugcaagc cuggaaaagg agccauuaua ucagcugugg     1260

cacacuauuu acucgcuaaa agaccucgau gaaugcaaga augcccugau aaaacgcuuu     1320

gguuucgacg augagacagc ugagaaacuu ucucauaucg acuuuaacaa acaggccuuc     1380

gggaacaagu ccaauaaguc aaugagaaaa auuuugccgu uucugaugga aggauacgac     1440

uauagucaga gcuguucccu cgcaggcuac aaccacucua auagccugac caaggaugag     1500

agggaacaac agaucacucu ugaccggcug gaguugcuca guaaaaacag ucugcgacag     1560

cccaucgugg aaaagauuuu aaaucagaug auaaacguag ugaaugcuau ccuggagcaa     1620

uauggcaaac uauccgagau ucgcgucgaa cucgccagag agcugaagca gagcaaggau     1680

gaaaggaacg acgcggaucu ucaaaauucu aaaaacaaga aauugaauga ggaaaucggg     1740

aagcggcugg ccgagcucgg acugccugug acaaaacgcu acauucagaa guauaaguuc     1800

aucuuuccau cauuaaguaa aaaccugaag gacgcacacg uuaauaacca gugcauauac     1860

uguggugaga ccuucaaucu uacggaggcu uugagcggcg augccuuuga cguggaucau     1920

aucgucccca aagcucuccu guucgacgau ucccagacca acaaggugcu aguacacaga     1980

aaaugcaacu cuacaaagac uaaucagacc gccuaugacu acauugggac aaagggagaa     2040

gaacagguua ggguguacuc ggagcguauc gaugacuggu uuaaacgggg cauuaucagc     2100

uauuccaaga ugcaacgacu gaaagucuca cauaaggaau accucgagag aaagaagcug     2160

gguaaagaga cugaaaccga caagaaacuu ugggagaacu ucauagauag gcaguugcgc     2220

gacacacagu auauuagucg gaaagcaaga gaaauccugc aaaaggugug uaauaacguc     2280

accacuacgg aagggggcgu gacagccaaa uuaaggaauc ucuggggaug ggaugacguu     2340

cugaugaacc uucagaugcc uaaguacaaa gagcugggcc agaccgugau uaaggaaugg     2400

acuucugagc acgggaagcg caaacaccag aaggaagaga ucgaaaauug gaccaaacga     2460

gaugaccauc ggcaccaugc uauagaugcg uugguagucg caugcacaaa gcaaggauuu     2520

auccagcgua uuaacacccu caauagcucc gacacuaaag aucugaugca gagagagauc     2580

aaggaggcca agaaacuacu gggugaaaac uauaaugaga aggaguucga aaacgacaau     2640

gaguucguga aaaaccacaa ggaaaggcuc ucacagcugg augacuaccu ucgcuugcaa     2700

aaaccaguua aguuugauac aaaguaugug gagcaggaag cugacaaagu ccuggugagc     2760

uucaaggccg gcaaaaaggu agcaacgauu accaaauuua aggccacugg gaagaauaaa     2820

gacacaggag ugaucguucc ccggggcgcu uuacaugagg ccggucucua cgggauaaga     2880

accauuuacu cuaagggcgg aaucgcagaa aaacacauca uuaagaaaua uaaggucggc     2940

guguccgcgc aggaucacau cuucacaggg aaagagacuu acagugugaa gaaggacaaa     3000

aacggaaacg aaaaggucga ugacaaaauu aagauaguuc uggauagcau cguggacaau     3060

aaagugaagg agguaguccu uaacaggcug aaucgagguu uuaacaauga ggaagauuau     3120

cgcauugacg ugaagaaagc ucuaaacaau uucaagaacc ucgaugagga cccgcugugg     3180

uacaaugaau cuaaaggcau caagauuuug uccaucaaac ugaagaccgg guuaaaggau     3240

ucaguugugc cucuccggcu gaacgagaaa aaugagguca uaacgcuugu gaagaccgga     3300

aacaaucauc acaucgccau uuaugaagac aacgacggca aacugaucca guacaguugu     3360

acauuuuggc augcaguuga gagaaagaaa uauaauauuc ccacugugau caagaacagc     3420

ucggaaguau ggaaucaagu cuuggauaag aaacucccag aguccuuccu gaacaggcua     3480

ccugccgaca agcugaaccu uaaauuuucu augcagcaga augaaauguu cauacucggu     3540

cuguuggagg aggaauuuga uaaggcuauu aaagacaacg auaagagccu guuaucaaaa     3600

uaccucuacc ugguguggag uaucgccgag ggggacuauu gguuccguca ccaccuugaa     3660

accaagaaua cagagcugaa gaaaacuaac ggcgcaaagg aauccaaacg cuacuuucgg     3720

uugucuacca agggacucau cgaucugaau cccgugaaag ucagacuaaa ccaucugggc     3780

gagauuacga agauagggga guauuga                                         3807


<210>  2912
<211>  3810
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2912
augaacaaua gcaucaaguc caaacccgag gugaccauug gccuggaccu cggggucgga       60

ucuguugguu gggccauagu ggauaacgaa acaaauauca uucaccaucu uggcucaaga      120

cuguucaguc aggcuaagac ugcagaggac aggcggagcu uucgcggggc ccgacguuug      180

aucagaaggc ggaaauacaa guuaaaacgc uucauuaacc ugaucuggaa guauaauucc      240

uacuuuggau ucaagaacaa agaagauaua cuaaauaacu aucaagagca gcagaagcuc      300

cacaauacgg uacugaaccu uaaacuggaa gcguugaaug cuaagaucga cccuaaagcc      360

cucucuugga uucugcauga uuaccugaag aacagaggcc acuuuuauga ggacaauagg      420

gauuucaacg uguacccaac cgaggaacuc gcaaauuauu uuggugagua cggguacuau      480

aagggaauca uugacucgaa agaagauaac gacgauaagu uagaggaggg ccugacaaaa      540

uacaaguucu caaaccagca uuggcuugaa gagaucaaaa aggucuugag caaucaaacu      600

ggccugccgg aaaaauuuaa ggaggaauau gagagucuau ucuccuacgu gcggaacuau      660

ucugaggggc ccggaagcau aaauuccguu ucaccuuacg guauuuauca ccucgacgaa      720

aaggagggca aaguggucca gaaguacaac aauaucuggg auaaaaccau ugggaagugc      780

aguaucuuuc cagacgaaua ccgcgccccc aaaaacagcc cuaucgcuau gauuuucaau      840

gagauaaacg aacugucuac aauccgaucc uauucaauuu accuuaccgg augguuuauc      900

aaucaggagu ucaagaaggc auaucugaac aaauugcucg accuguuaau aaagacuaau      960

agcgagaaac caauugaugc cagacaguuc aagaaacuga gggaagagac aaucgcugaa     1020

aguaucggca aggagacccu caaggacgug gaacgggagg agaaacuuga aaaggaggau     1080

cacaaaugga agcugaaagg uuugaagcua aacacgaaug ggaagauuca auacaacgac     1140

cugucuuccc ucgccaaauu uguacauaag cugaaacagc accuuaagcu ggauuucuug     1200

cucgaagacc aguauacuac ccuggauaaa aucaauuuuc uucagagccu guucguuuac     1260

cuaggaaagc aucuccguua uucgaaccgc guggacucug cgaaccugaa agaguuuucc     1320

gauucaaaua aacuuuucga gagagucuug caagaacaga aggacgaucu guuuaaacuc     1380

uucgagcaga cagacaagga cgaugaaaag auucuggcac aaacucacag ucuuagcacc     1440

aaggccaugc uguuggcuau aacaaggaug accaaccucg acaaugauga ggacaaccag     1500

aaaaauaacg auaagggcug gaauuuugaa gccaucaaga acuucgacca gaaauuuauu     1560

gauaucacga agaaaaauaa caaucugucc uuaaagcagg acaaacggua ccuggaugac     1620

cgauucauca acgaugcaau ucuaucuccc ggcgugaagc gcauacucag agaggcuacu     1680

aagguguuua augccauccu gaaacaauuc agcgaggaau acgacgucac aaagguugug     1740

auugagcuug cgagggaauu gucagaggaa aaagagcugg agaacaccaa gaauuaugaa     1800

aaacucauca agaagaacag ugauaaaauu uccgagcggc uggaagccuu agacaucgca     1860

gaggacaaga uagaagauau ccugaaaagc ccuacuaagu cuuacaaagu acuuuugugg     1920

cuccagcagg accacauuga uccauauuca cagaaggaga ucgcuuucga ggacauucug     1980

acaaaaaccg aaaagacgga gaucgaucau auaauuccgu acaguaucag cuuugacgau     2040

uccucuucga acaagcuacu ggugcucgcc gaaagcaauc aagcuaaauc caaccagacc     2100

cccuaugagu ucauuacauc agggaaugcc ggaaucaagu gggaggacua cgaagcauau     2160

ugucgcaaau uuaaggaugg ugacacuuca cugcuugauu cuacccagag aagcaaaaag     2220

uucgccaaga ugaugaaaac agacacuucc ucaaaguacg auauaggcuu uuuggcuagg     2280

aaccugaaug acacccguua cgcgacaauc gucuuccggg acgcauuaaa agauuaugcc     2340

aacaaucacc ucguggagga caagccuaug uuuaaaguug ugugcauuaa cgggggaguc     2400

accagcuucc ugcgaaagaa uuuugauaag ucuugguacg cuaaaaagga cagagauaaa     2460

aacauccauc acgccgugga cgcauccauu aucaguauau ucagcaauga aacuaagacg     2520

cuuuuuaacc agcugacaca auucgccgau uauaaauugu uuaagaauac cgacggcucu     2580

uggaagaaaa uugauccaaa gacuggugua guuaccgagg ugacagacga aaacuggaaa     2640

cagaucaggg uccgcaauca gguguccgag aucgcuaagg ucauuaacaa auguauccag     2700

gauucaaaca uugagcggaa ggccagauac aguaggaaga uagaaaauaa aaccaacauc     2760

agccucuuca augacacugu guauucggca aagaaaguug gguacgagga ccaaauuaag     2820

cgcaaaaacc ugaagacacu agauauccau gaauccgcgg aggaaaauaa aaacucuaag     2880

gugaagaaac aguucguaua ucgaaagcug gucaauguga gccuccugaa caaugacaaa     2940

cuugcugauu uguuugccga gaaggaggac auucugaugu accgggcaaa ccccuggguu     3000

aucaauuuag ccgaacagau auucaacgag uacacggaaa auaaaaagau caagucacaa     3060

aacguguuug agaaauauau gcucgaucug accaaggagu ucccugaaaa auuuagugag     3120

gcuuucguca aguccaugcu ucguaauaaa acugccauua ucuacaacgu ggaaaagaag     3180

guagugcaca gaauuaaaag gcugaagauc cuaucuagcg agcucaaaga aaauaagcug     3240

uccaacguua uaauucgcag uaaaaacgag agcggcacaa aguugaguua ucaggacacc     3300

aucaauucua ucgcacugau gauuaugcgg uccauagauc caacagcuaa gaaacaguac     3360

aucagagucc ccuuaaacac ucucaaucug caccuuggag accaggauuu ugaccugcau     3420

aacauugaug ccuauuugaa gaaaccgaag uucgugaaau accucaaggc gaaugagauc     3480

ggcgacgaau auaaaccuug gagggugcug accagcggga cgcuacugau ucacaagaag     3540

gauaaaaagu uaauguacau cucgucauuu caaaaccuca aagacgucau agagaucaag     3600

aaucugauug aaaccgagua caaagaaaac gacgauucug accccaagaa gaaaaagaaa     3660

gccaaucgau ucuugaugac acugaguacu aucuuaaacg auuauauucu ccuggacgcu     3720

aaggauaauu uugacauccu uggacugucc aaaaaccgca uagaugagau uuugaauagc     3780

aagcucgguc uggacaagau caccaaauga                                      3810


<210>  2913
<211>  3810
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2913
augaacaaua gcaucaaguc caaacccgag gugaccauug gccuggaccu cggggucgga       60

ucuguugguu gggccauagu ggauaacgaa acaaauauca uucaccaucu uggcucaaga      120

cuguucaguc aggcuaagac ugcagaggac aggcggagcu uucgcggggu acgacguuug      180

aucagaaggc ggaaauacaa guuaaaacgc uucgugaacc ugauuuggaa guauaauucc      240

uacuuuggau ucaagaacaa agaagauauc cuaaauaacu aucaagagca gcagaagcuc      300

cacaauacgg uccugaaccu uaaaucugaa gcccugaaug cgaagauaga cccuaaagca      360

uugucgugga uccuccauga uuaccugaag aacagaggcc acuuuuauga ggacaauagg      420

gauuucaacg uguacccaac caaggagcug gccaaauauu uugacaagua cgguuacuau      480

aaagggauua ucgauucaaa ggaagacaau gauaacaaac ucgaggaaga guuaacaaag      540

uacaaauuca gcaacaagca uuggcuggag gaaguuaaga aagugcuuag uaaucagacu      600

ggauugccgg agaaguuuaa agaagaguau gaaucccugu ucucuuacgu ccggaacuau      660

agcgagggcc ccggcuccau uaauucagug aguccuuacg ggaucuauca ccuagacgag      720

aaggaaggaa aaguaguuca aaaguacaac aauauauggg auaagaccau ugguaaaugc      780

aacaucuuuc cagacgagua ccgcgcaccc aagaauagcc cuauugccau gaucuucaac      840

gaaaucaaug agcucucuac aauucgaucc uauucaauau accugaccgg cugguuuauc      900

aaccaggagu ucaaaaaggc uuaucuuaau aaacuguugg accuccugau uaagacuaac      960

ggggaaaagc caaucgaugc aagacaguuc aaaaaguuaa gggaggaaac aauagccgag     1020

agcauuggaa aagaaacccu gaaggacgug gagaaugaag agaaacucga gaaggaagau     1080

cacaagugga aacuuaaggg ccugaaauug aacacgaaug guaagaucca guacaacgac     1140

cuaaguucuc uggcuaaauu uguccauaag cucaaacaac accugaaacu ugauuuccug     1200

uuggaggacc aguaugccac ucucgauaag aucaacuuuc ugcaguccuu auucguguac     1260

cuggggaaac aucuacggua uaauaaccgu guggacagcg cgaaucucaa ggaauuuucg     1320

gauucuaacc gccuguucga gagaguccuu caggagcaaa aagacggauu guuuaagcug     1380

uucgaacaga ccgauaaaga cgacgagaag auucucgcac agacacacuc ccugucaacu     1440

aaggccaugc uucuggcuau caccaggaug acaaauuugg auaacgacga agauaaucaa     1500

aagaacaaug acaagggcug gaacuuugag gccauuaaaa auuucgauca gaaguuuaua     1560

gacaucacca aaacgaacaa uaaccucagu cugaagcagg auaagcggua cuuagacgau     1620

cgauucauua augacgcaau ccugagcccc ggcguuaaac gcauccuaag agaagcuacu     1680

aagguguuua acgccauucu caaacaguuc ucccaagagu acgauguaac aaaggugguc     1740

auagagcugg cgagggaacu uucugaggaa aaagaguugg aaaacaauaa gaacuauaag     1800

aaacugauca agaaaaauag cgacaagauu ucagaggggc ucaaagcccu ggauaucgca     1860

gaggacaaga uugaagacau cuuaaaaagu ccuaccaagu ccuacaaggu gcugcuuugg     1920

uugcagcagg aucacauaga cccauauagc cagaaagaga ucgcuuucga agauauucuc     1980

acuaagacag agaaaaccga aaucgaccau auuaucccgu acucuauauc auuugaugac     2040

aguagcucca acaagcugcu aguucuggcc gagucuaauc aagcuaaauc gaaccagacg     2100

cccuaugagu ucauuaccag cggaaaugcc gguaucaagu gggaagauua cgaggcauau     2160

ugucggaagu uuaaagacgg cgauacaucc cuccuggacu caacucagcg caguaagaaa     2220

uucgccaaga ugaugaaaac cgauacaucu agcaaguacg acauuggguu ucuugcuaga     2280

aacuugaaug auacuaggua cgcgaccauc guguuccgug acgcacugaa ggacuaugcc     2340

aacaaucacu uagucgaaga uaaaccuaug uuuaaggugg uaugcauaaa cggaggcguu     2400

acauccuucc uccggaaaaa uuuugacaag ucaugguacg cuaaaaagga ucgagacaag     2460

aacauccauc acgccgugga ugcaagcauu aucucuauuu ucuccaauaa aaccaagacu     2520

cuguuugacc agcuuacgca auucgccgau uauaaacugu uuaagaacac agacgguagu     2580

uggaaaaaga ucgauccaaa gaccgggguc gugacugagg ucaccgacga gaacuggaaa     2640

cagauaagag ugaggaauca gguuagcaag auugcugaag agaucgauaa auguauccag     2700

gacucuaaca uugaacgcaa ggcccgguac uccagaaaaa ucgagaauaa gacaaacauu     2760

ucauuguuca augacaccgu guauagugcu aaaaagguag gcuacgauga ccaaauaaag     2820

aggaaaaacc ucaagacucu ggauaucgac gaaagcgucg aggagaauaa aaacucgaag     2880

gugaaaaagc aguucguuua ucgcaagcua gugaaugucu cccugcucaa caaugauaaa     2940

cuggcggacc uuuuugcuga aaaggaggau auuuugaugu accgagccaa ccccugggug     3000

aucaaucugg cagaacagau uuucaacgag uacacagaaa aucggaaaau caagucucaa     3060

aacguauuug gaaaauauau guuagaccuc acgaaggagu ucccugagaa guuuagcgaa     3120

gccuucguga aaucaaugcu gcguaauaag accgcuauaa ucuacaacgu ugagaaaaag     3180

gucgugcaua gaauuaaaag gcuuaagauc cugaguuccg aacuaaagga gaacaaacuc     3240

ucuaauguga uuauccgcag caagaacgaa uccggcacua aacugucaua ucaggauaca     3300

auaaauagcg ucgccuugau gauuaugcgg aguaucgacc caaccgcaaa gaaacaguac     3360

aucagaguuc cccugaacac auuaaaucuc caccuggggg aucacgacuu ugaucuucau     3420

aacauugacg cuuaucugaa gaaaccgaag uucgugaagu acuugaaagc caaugagaua     3480

ggagacgagu auaagccuug gagggugcuc aucucugguu cccugcuaau ucacaaacga     3540

gauaagaaac ugauguacau cagcucguuu cagaaccuua augaccucau ugaaaucaag     3600

aaccugauag agacugaaua caaggagaau guagauucag accccaaaaa gaaaaagaaa     3660

gcgucucaaa ucuugcgcag ucuguccacc auuuuaaacg auuauauccu ccuggacgcc     3720

aaggauaauu ucgacauucu uggccugagc aagaaccgga ucgaugagau acucaauucu     3780

aaauuggacc uggauaagau ugcuaaauga                                      3810


<210>  2914
<211>  3822
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2914
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugauagag       60

guggaugaaa auaacuuccc caucagaauu aucgcuaugg guuccaggau uaucccuuug      120

ucuucaaaug acaaggauga guuucagcgg ggcuuaagua uaacaaagaa ccaaagccgc      180

acugucuccc gaacgcagcg uaagggguac gacagaaaac agcugaagaa gucugaugac      240

uucaaauauu cgcuaaggcg gauccucaau aaguacaaca uuuuuccauc agaggagcug      300

cuuaaacugc cgauguugga ucucuggaag cugcgcagcg acgcaguuaa ucccaacgaa      360

aauaucagug ccgagcagcu gggacggauu cucuauaugu uaaaccagaa aaggggcuac      420

aaguccgcgc ggucugaggc uaaugccgau aagaaagaca ccgauuaugu gcuggcagua      480

aagggucgcu acgcccagcu uaaagaccga aaccagacaa ucgggcagua uuucuacgaa      540

gaguugcaca augcuaagac uaccaacagc uacuuuagaa caaaagaaga gguguauccu      600

agggaggcau acauagaaga guucgauacc auuaucaaug cccaaaaguc caaacaugac      660

uuucugacug acgaagucgu gcaccagcua cggaacgaga uuaucuauuu ccagcguaag      720

cucaagucac agaaaggacu gguuaguauc ugcgaauuug agggcuucga gaagacaauu      780

uuugauaaag aaaaggacaa aaacaaggug gucuucgugg gcccaaaggu agcucccaaa      840

accagcccuc uuuuucaacu gugucgcaua ugggagguug ugaauacgau cacuuugaag      900

auuaaaaacc cagaaggguc uaaguacaaa uggggagaga agauccccaa uauaaaggaa      960

aaagaggccc ucgcggauua ucuguucaag aacgagaccu uauccuucaa uaaacugcuc     1020

gacauucuug aacugaagaa agagaacguc uacgugaaua agcagaucau caaggguauu     1080

aaaggcaacg aaacauauuc agcauugcau agaauccuag gggauaauga gcugcucaag     1140

uuugacguga gcauuauacc uaguaaccac acuucuaugc uggucgauaa aaagaccgga     1200

gaaauccuug aggagagggc cggccuggaa uuggacguuu cccucgagaa agaaccacug     1260

uaccaguuau ggcauacaau uuacagcauc aaggaucugg acgagugcaa aaaugcucua     1320

aucaagcggu ucgguuuuga ugacgagauu gccgaaaagc ucucgcaccu ggacuucaac     1380

aaacaagcau uugggaauaa gucuaacaaa uccaugcgaa agauacuucc guucuugaug     1440

gagggauaua auuacucaca gaguuguacc cuggcuggcu auaaccacag caacucccuc     1500

acgaaagaug aacgcgagca acaggugacu cuggacaaac uugaacuguu gcccaaaaau     1560

ucucucagac aaccuaucgu agagaagauu cugaaccaga ugaucaaugu ggucaacgcc     1620

auuuuagagg aauacggcaa gccaagcgag aucaggauag aacuggcgcg ggagcuaaaa     1680

cagucaaagg augaacgcca ugacgccgau cuccagaaua guaaaaacaa gaagcugaau     1740

gaggagaucg ggaaaagacu uacagaauug ggacugcccg ugaccaagag guauauucaa     1800

aaauacaagu uuaucuuccc uucccucagc aaaucucuga aggacgcaca gguuaacaau     1860

cagugcauuu auugugguga gacuuuuaac uuaacagaag cucugucagg cgaugccuac     1920

gacguggauc acaucguccc aaagagucuu uuguucgacg auagccagac caauaaagug     1980

cucguacauc guaagugcaa cuccacgaaa accaaucaaa cagcuuacga cuauauagcc     2040

ucuaaggggg aggaagaggu ucagauuuac ucggcacggg uggaugacug guuuaaacga     2100

ggaaucauua gcuauuccaa gaugcagaga cugaaagucu cacacaagga guaccuagaa     2160

aggaagaguc ugaacaaaga gacugaaacc gacaagaaac ucugggagaa uuucaucgau     2220

cgccagcugc gggacacaca auauauaucu agaaagagca aagagauccu ucagaaggug     2280

uguaacaacg ucacuaccac agaaggcggu gugaccgcca aguugaggga ucuguggggg     2340

ugggacgaua uuuuaaugaa ucuccagaug cccaaauaca aggagcugcg ccaaacuguu     2400

aucaaagaau ggacguccga gcacggcaag cgaaaacauc agaaggaaga gauugagaac     2460

uggacaaagc gggacgauca ccgucaucac gcuaucgacg cgcuugugau agcaugcacc     2520

aaacagggau uuauucagag aaucaauacu cugaacucaa gcgaaaccaa ggauuugaug     2580

caacaggaga ucaaugaagc caaaaagcuc cugggcgaga acuauaaaga caaggaauuu     2640

gaaaauucua acgagcuagu aaagaaucau aaagggaggc uguccaagcu cgaugacuac     2700

cugcgccuuc agaaaccgau uaaguucaac acaaaauacg ucgaaaagga ggcugauaag     2760

aucuugauua guuucaaagc cggaaagaaa guggcaacca uaacuaaguu caaagccaca     2820

gguaagaaua aagacacggg cguuaucgug ccuagagggg cucugcacga agccggauua     2880

uauggcguca gaaccauuua cagcaagggu gggaucgcag agaagcacau uaucaaaaag     2940

uauaaagugg gcguaucugc gcaggaccau auauuuacug gaaaggaauc cuacucagug     3000

aaaaaggaua agaacggcaa ugagaaaguu gacgauaaga ucaaagucgu gcucgacagu     3060

auuguggaua gcaaggucaa agagaucguu cugaacaggc uuaauuucgg guuuucggac     3120

ggagaagauu auaagaacaa cgugaagaaa gcucugaaua acuucaagaa ucuagacgag     3180

uacccccucu gguauaacga aaauaaaggu auuaagaucc uguccauaaa auugaagaca     3240

ggccugaagg auucuguggu accauuacga cucaacgaga gcaaugaggu cauuacccug     3300

gugaaaacag ggaacaauca ccauaucgcc aucuacgaag acaagaacuc aaaaccuguu     3360

caacacaguu guacuuuuug gcacgcagug gagcgcaaga aauacaauau ucccgccguc     3420

auaaagaaau ccucugaugu guggaaccag cuucuggaca aggaauugcc agagagcuuc     3480

cucaauaacc ugcccgaaga caaucuaaac cugaaguuuu ccaugcagca gaaugagaug     3540

uucguucuug gacucucaca agaggaauuu aaagaggcua ucgaaaacaa cgauaagagc     3600

cuguugagua aauaucugua cuuagugugg ucuguauccg agaauaacua uugguuccgg     3660

caucaccucg aaaccaagaa uagcgaccug aaaaagacgg agggcgccaa ggagucgaaa     3720

agacuuuaca acauuagguc agucucuagu cuguuuuccu ugaauccugu gaaggugcgu     3780

cucaaccauc ugggugaaau caccaaaauu ggggaguauu ga                        3822


<210>  2915
<211>  3825
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2915
augaccaagc acauccuggg ccucgaccuu gggacaaaca gcauuggaug ggccauaauc       60

cagguggaua auaacaauaa cguccccauu caaaucauug cuauggguuc cagaaucaua      120

ccucuggacu cuaaugauag ggaccaguuc cagaaaggcc aggcaaucuc aaagaacaaa      180

gaucggacua cggcccgcac ccaacgaaag ggguacgacc guaaacaguu gaagaagagu      240

gaugacuuua aauauagcuu aaagaaaauu cuggagaagc uagauaucuu cccaacagaa      300

gaacucauga aacugccgac ucuugaccug uggaaguuga gauccgaugc gguuucuaau      360

auugaagaca ucacccccaa gcagcucgga aggauacugu acaugcugaa ccagaaacgg      420

ggcuauaagu cggcucgcuc agaggccaau gcagauaaaa aggacacaga cuacguggcc      480

gaaguaaaag guagauauac ccaacucaag gauaaagggc agacuuuagg acaguacuuu      540

uauaaggagc ugagcgacgc uaaccagaau aacacauacu acagggugaa ggagaaaguc      600

uauccucggg aagcauacau ugaggaguuc gaugccauca uuaaugugca aaagaguaaa      660

cauuccuuuc uuaccgacga gguuauccac ucuuugcgca acgagaucau uuauuaccag      720

cgaaagcuga aaagccagaa gggccuagug uccauaugcg aguucgaagg cuuugaaacg      780

acuuauuucg auaagaaaac ccaacaggac aagacaaucu uuacugggcc aaaagucgcu      840

cccagaaccu caccucucuu ccaguuuugu aagauuuggg aggugguaaa uaacaucagu      900

cugaaaacaa agaacccaga aggaagcaag uacaaauggu cugauaggau acccaccauu      960

gaggagaagc agacgaucgc caauuaucuu caagaaaacg agaaucuguc cuucaucgaa     1020

uugcucaaaa uucugcaguu aaagaaagag cagguuuacg cgaacaagca gauccugaag     1080

gguauucaag gcaauacuac auuuucagca auacauaaaa ucauugggaa cagcgaacac     1140

cucaaguucg acaucgagac caucccuagu aaacauuucg ccgugcuugu cgauaagaaa     1200

acuggagaga uucuggacga acgggauucu uuggagcuaa auuccgcucu ggaacaggag     1260

ccauuuuacc agcucuggca cacaauauau agcaucaagg accuggacga augcaaaaag     1320

gcccuuauua agcguuucaa cuuugaggag gaaaucgcag agaaacuguc gaagauugau     1380

uucaauaaac aggcuuuugg caacaagucu aauaaagcca ugcgcaagau guugccguac     1440

cucaugcugg guuauaacca auccgaagcg gagucauucg ccggguacaa uagaagguua     1500

accaaggagg agaaaaguaa gaacgugagc gacgaacccc ugcagcuacu cgcuaaaaau     1560

ucccugcggc agccuguggu cgaaaagauc cuuaaccaga ugauaaacgu ugugaaugca     1620

aucauugaga aauauggaaa gccagaagag auccgaguag aguuggcccg cgaacugaag     1680

caaucuaaag augagagaga agacgcugau aagcagaacg gcuuuaauaa aaagcucaac     1740

gagcuggugg ccacgaaacu uaccgaacug ggcuugccca caacuaagca cuacauucag     1800

aaguauaaau ucaucuuucc ugcaaaggac aaaaauugga aggaggccca agucgcuaac     1860

caguguauau acugcgggga uaccuucaau cucacagagg cgcugagcgg agacaacuuu     1920

gauguggacc auauuguucc aaaagcauua cuguucgaug acucacaggc caauaaggug     1980

cuaguccaca ggaguuguaa cuccacuaaa accaauaaca cagcuuacga uuauaucacc     2040

aagaagggua gccaggcccu caaugacuac guggcacggg uagacgauug guuuaaacgc     2100

ggcauuaucu cuuaugggaa gaugcaaaga cugaaaguuu cauucgaaga guaccaggaa     2160

aggaagaaaa uaggaaagga gacugaagcc gacaagcgua ucugggagaa cuucauugau     2220

cggcagcuuc gagagacggc uuauaucgcc aaaaaggcaa aagaaauuuu ggagaaggug     2280

ugccauaaug ucacaaguac cgaaggcaac gugacugcga aacugagaca gcucuggggu     2340

ugggacaacg uccugaugaa uuuacaacug cccaaguaca aggagcuuga gaaaaagacc     2400

aaacagacau uuacccaguu gaaggaaugg acuagcgauc acgggaacag gaaacaccag     2460

aaggaggaaa ucauaaauug gacaaagcgc gacgaucauc ggcaccaugc uauugacgcc     2520

cucgugaucg cauguacgca acagggcuuc auccagagaa uuaacacccu guccucuucg     2580

gauguuaaag acgagaugaa gaaagaacua gaggaggaua agacugugua uaaugaaagg     2640

cugacacucc uggagaacua ccuuuuggag aaaaagccgu uuagcaccga agaaaucgag     2700

aaggaggccg acaaaauucu gguauccuuc aaggcuggca aaaaggucgc cacauuauca     2760

aaauacaagg caacuggaau aaaugaaauc aaaggggugc ucguuccucg cggaccccug     2820

cacgagcaaa guguguaugg uaagauuaag gucaucgaaa aagauaagcc acuuaaauac     2880

cuguuugaga acucugacaa gauugugaau ccuuugauca aacaucucgu aaagacccga     2940

cugcuagaaa acgagaauaa cgcucaggcc gcgcugguga cgcucaagaa uaaacccaua     3000

cugcuuaaca auaagcagac cgagaucuug gaaaaagcca gcugcuauaa cgaggcuaca     3060

guucugaagu acaaauuaca gucccucaag gcaucacaaa uugacgauau cgucgacgaa     3120

aagauuaaau uccugaucaa ggagcggcuu agcaaauuug gcaacaagga gaaagaagcc     3180

uucaaggaua uacugugguu uaaugagaag aaacagauuc caaucacuuc uauccgucua     3240

uucgcuagac ccgacgccaa caaucuccag gugauuaaga aacacgaaaa ggggaaaaac     3300

auaggauuug ugcuguccgg caauaaccac cauaucgcaa uuuaugagga uaagaauaac     3360

aaauugaucc agcacauuug ugacuucugg caugcggucg aaaggaagcg caauaacauc     3420

ccuguucuga uagaggauac caguacaauc uggaaucacu uaauuaacga ggacuuuagc     3480

gaaucuuucc ucaauaagcu gccaaacgau ucccuuaaac ugaaguucuc auugcaacag     3540

aaugagaugu uuauccucgg ucugccgaaa gaacagagug aggaagccau uaagagcaac     3600

aauaaaucgc uacuguccaa gcaccuuuac cucguguggu cuaucacuga cggggauuau     3660

uucuuucggc aucaccugga gaccaagaac acggaguuga aaaagauaga cggcagcaaa     3720

gaaucaaaga gauaccugag guuaaguaca aaaucccucg uggaccugaa ccccauuaag     3780

guacgacuua aucaucuggg agagaucacc aagaucggcg aauga                     3825


<210>  2916
<211>  3846
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2916
auggaggacg auagccagca cgacaucaga gaaauuuacc uggccauaca ucacauggug       60

aaguauaggg gcaacuuccu cgucgagggg acccuugaau ccucuaaugc uuuuaaagag      120

gaugaacugu ugaaguuacu gggacggauc acacgcuacg agaugucaga gggugaacaa      180

aacagugaca uugagcagga ugacgaaaau aaacuuguug cacccgccaa cggccagcuc      240

gcggaugcuc ugugcgccac ucgagggagc cguuccauga gaguggacaa ugcacuugag      300

gcccugucug cuguaaacga uuugucgagg gagcagcggg caaucgugaa ggccauuuuc      360

gcuggacucg aaggcaauaa acuggaccug gccaagaucu uugucucaaa ggaguucagc      420

agugaaaaca aaaagauacu cgguaucuau uuuaauaaau ccgauuacga ggaaaagugu      480

gugcaaauug uugacucugg guuacuggau gacgaggagc gcgaguuccu ugacagaaug      540

cagggacagu auaacgcgau cgcauugaaa cagcugcuag gcaggagcac guccguguca      600

gauaguaagu gcgccagcua cgacgcucau cgggccaauu ggaaccucau uaagcugcaa      660

cuucgcacca aagaaaauga aaaggauauc aacgagaauu auggcauacu ggucgggugg      720

aaaauugacu cuggacagcg aaaguccgug agaggugaau cagcauacga gaacaugagg      780

aaaaaggcua acguauuuuu caaaaagaug aucgagacaa gcgauuugag ugaaacugac      840

aagaaucggc ucauucacga uaucgaggaa gacaaacugu uuccuaucca gcgugauucu      900

gacaacggcg uuauuccaca ucaguuacac caaaaugagc ugaagcagau aaucaaaaag      960

caggggaaau acuauccguu ccuccuugau gccuuugaaa aggacggaaa gcaaauuaac     1020

aaaaucgagg gccuguuggc guuccgcgug cccuacuuug ucgguccucu aguggugcca     1080

gaggaccugc agaaguccga uaauagcgaa aaccacugga uggucaggaa aaagaaaggg     1140

gagauaaccc ccuggaauuu cgacgaaaug guugauaagg acgccucggg aagaaaguuc     1200

auugagcggc ucgugggcac agauucuuau cugcuuggcg agccuacccu gcccaaaaac     1260

uccuugcucu accaggaaua ugagguacug aaugaauuaa acaaugugcg acugucaguc     1320

cgcacuggga accauuggaa ugacaagaga aggaugcggc uaggacgcga ggagaaaaca     1380

cuccuguguc agagacuuuu uaugaagggu caaaccguga cgaaaaggac ugcagaaaac     1440

uugcugcgua aggaguacgg ccggaccuau gaacucagug ggcugagcga ugaguccaag     1500

uucacaucua gccuuucaac uuacggaaaa augugccgaa ucuuuggcga aaaguacguu     1560

aaugagcaca gagaccugau ggagaaaauc guggaauugc agaccgucuu cgaggauaag     1620

gaaacacucc ugcaucaguu aaggcagcug gaggguauua gugaagcuga cugugcccua     1680

cucgugaaca cccacuauac gggguggggc cgccuguccc ggaaacuuuu gacuacaaag     1740

gcuggagagu gcaaaaucag cgaugacuuu gccccaagaa agcacucuau uauagaaauc     1800

augagggcag aggaucgcaa ccugauggag auuaucaccg acaagcaacu cggcuucuca     1860

gacuggaucg aacaggagaa ucugggggcc gaaaacggaa guagcuuaau ggagguaguu     1920

gaugaccugc gagugucccc uaaagucaag cgggguauua uacagucuau ccgucuuauu     1980

gaugacaucu cgaaagcugu gggcaagaga ccaagcagga ucuuuuugga gcucgcggau     2040

gacauucagc cguccgggcg cacuauauca cggaaaagua gacugcaaga ucuauacagg     2100

aaugcaaacc ugggaaagga guucaagggc aucgccgacg aacucaaugc uuguucugau     2160

aaagaccugc aggaugaccg acuauuuuug uauuacacac agcuggguaa ggauauguau     2220

accggggaag aguuagaccu cgaccgccug agcuccgccu acgauauuga ccauaucauu     2280

ccccaggcag ucacgcaaaa cgauucaauc gacaaucggg ugcuuguugc cagagcugaa     2340

aacgccagga aaaccgauag cuucacauau augccucaga uagcagaccg uaugcgcaau     2400

uuuuggcaga uucuguugga uaacggccuc aucucucggg ugaaguucga gagacugacu     2460

aggcaaaaug aguuuuccga acgagagaaa gaacgguucg uacagcgcag ucuagucgag     2520

accagacaga uuaugaagaa cguggcgaca cugaugaggc agcgguacgg aaauagcgcu     2580

gccguuaucg gccucaacgc agaacugacu aaggagaugc accgauaccu uggguucucu     2640

cauaaaaaua gagacauaaa cgauuaucac caugcccaag acgcuuugug cgugggaauc     2700

gccggucagu uugcagcuaa ccgcggcuuc uuugccgaug gggaggucuc cgacggagcg     2760

caaaauucau acaaccagua ucugagggac uacuuacgug gcuaucggga aaagcucagu     2820

gccgaggaua gaaaacaggg uagggcuuuc ggguuuauug ugggcagcau gcgcucgcag     2880

gacgaacaga agcgaguaaa uccaagaacc ggagaggugg uuugguccga agaggauaaa     2940

gacuaccugc ggaaggucau gaacuauagg aagaugcuug ugacacagaa agugggcgau     3000

gacuucgggg cacuguacga ugagacccgc uacgccgcua cugaccccaa gggaaucaaa     3060

ggcauuccuu uugauggugc caagcaagac acgucucuau augggggauu cagcucagca     3120

aaaccagcgu acgccguccu caucgaaagu aagggcaaaa cacgucuggu uaaugugacc     3180

augcaggagu auuccuugcu gggugauaga cccucugacg augaauuacg gaaggugcuc     3240

gcuaagaaaa agagcgagua cgccaaagca aacauacugc uuaggcacgu accgaagaug     3300

cagcugauuc gcuauggcgg gggauugaug gucaucaaau ccgcuggcga gcucaauaac     3360

gcccaacagc uguggcuacc uuacgaagag uauuguuacu uugacgaccu gucacagggg     3420

aagggaagcc uugaaaagga ugaccucaaa aagcuguugg auaguauccu gggcucugug     3480

cagugcuuau accccuggca ccgauucacu gaggaggaac ucgcagaccu gcauguugcc     3540

uuugauaaac uuccagagga cgaaaagaaa aaugugauua ccgguaucgu cuccgcucug     3600

cacgcggaug ccaagacagc aaacuugagc auugugggga ugaccggauc guggagacgg     3660

augaauaaca agucaggcua uacuuucucu gacgaggaug aauuuauauu ccaaaguccu     3720

uccggucucu ucgagaaaag gguuacagug ggcgaacuga agcgcaaagc uaagaaagag     3780

guaaauagca aguacagaac gaacgagaag aggcuaccca cccugucugg ggccagccag     3840

ccauga                                                                3846


<210>  2917
<211>  3861
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2917
augaccaaga acauccuggg ccucgaccuu gggacaaaua gcauuggaug ggcccugaua       60

gagguggaug aaaacaauac ucccaucaga auuaucgcua uggguuccag gauuaucccu      120

uugucuacgu cagacaaaga ggaguuccag cggggcuuaa guauaaccaa gaaccaaagc      180

cgcacagucu cccgaacuca gcguaaaggg uacgauagaa agcagcugaa aaagucugac      240

cuaaagaaaa uccucaauaa guauaacauu uuuccaucgg aggaacugcu uaaacugccg      300

auguuggauc ucuggaagcu gaggucagac agcgcaaauc ccaacgagaa uaucagugcc      360

gagcagcugg gacggauucu cuacauguua aaccaaaaac gcggcuauaa guccgcgaga      420

ucugaagcua augccgauaa gaaagacacc gauuacguuc aggcagugaa ggguagguau      480

gcccagcuga aagagcggaa ccagacaauc gggcaauacu ucuaugagga gcuuuacaau      540

gcuaacacca auaacagcua cuuucgcaua aaggaggaag uauauccucg agaggcauac      600

acugaagagu ucgacacaau uaucaacgcc cagaaaaaua agcacgacuu uuugaccgau      660

gaaguggucc aucagcugag aaacgagauu aucuauuucc agaggaaacu aaagucccaa      720

aagggacucg ugucaaucug cgaguuugaa ggcuucgaga aaacgguuuu ugauaaggaa      780

aagaauaagg acaaaguggu cuucgugggc aguaagguag cuccaaagac uagcccccug      840

uuucagcuuu gucggauuug ggagguugug aacaccauau cucugaaaau caagaauccu      900

gaagggucca aauacaagug gggagagaaa acaccaaacu ugaaggagaa ggaaauucuc      960

gccgacuauc uguucaaaaa ugauucacug agcuuuacua aguuacucga gauccuugac     1020

cugaacaaaa augaugucua cgugaacaag cagauauuga aagguauuaa gggcaaugaa     1080

accuucagug cgcuacacac agugcugggg gacaacgagc uccugaauuu cgaugucucu     1140

aucauccccu ccaaccauac cagcaugcuu guugacaaga aaacgggaga aauucuggag     1200

gagcgugcag gcuuggaacu cgaugccucg cuggagaagg aaccuuuaua ucaacugugg     1260

cacacuaucu acucucuaaa agugcucgac gagugcaaga augcucugau uaaacgcuuu     1320

gguuucgaug acgagacagc cgaaaaacuu ucccauauag auuuuaacaa acaggcauuc     1380

gggaacaagu caaauaagag uaugagaaaa aucuugccau uucugaugga gggauacgac     1440

uauagccagu ccuguucucu cgcuggcuac aaccacagca auucacugac caaggacgaa     1500

agggagcagc aaauuacucu ugaucggcug gaauugcuca guaaaaacuc ccugcgacag     1560

ccgaucguag agaagaucuu aaaucagaug auuaacgugg ucaaugccau acuggagcag     1620

uauggcaaac uaagcgaaau ccgcguggag cucgcgagag aacugaagca aucuaaggac     1680

gagaggaacg augccgaccu ucagaauagu aaaaacaaga aauugaauga agagauuggg     1740

aagcggcugg cagagcucgg acugcccguu acaaaacgcu acauccagaa guauaaguuc     1800

auuuuuccuu cauuaagcaa aaaccugaag gaugcucacg ugaauaacca gugcaucuac     1860

uguggugaaa ccuucaaucu uacggaggcc uuguccggcg acgcuuuuga ugucgaccau     1920

auagugccaa aagcccuccu guucgaugac ucucaaacca acaagguucu aguacacaga     1980

aaaugcaacu cgacaaagac uaaucagacc gcauaugauu acaucgggac aaagggagaa     2040

gaacagguga ggauuuacag cgaacguguc gacgauuggu uuaaacgggg caucauuucc     2100

uauucaaaga ugcagcgacu gaaagugagu cauaaggagu accucgagag aaagaagcug     2160

gguaaagaaa cugagaccga caagaaacuu ugggaaaacu ucaucgacag gcaauugcgc     2220

gauacacagu auauaucucg gaaagccaga gagauucugc agaaggucug uaauaacgug     2280

accacuacgg aagggggcau cacagcuaaa uuaaggaauc ucuggggaug ggacgauguu     2340

cugaugaacc uucaaaugcc caaguacaaa gaacugggcc agaccgugau uaaggagugg     2400

acuagcgaac acgggaagcg caaacaccag aaggaggaaa ucgagaauug gaccaaacga     2460

gacgaucauc ggcaccaugc gauagacgca uugguagucg ccugcacaaa gcagggauuu     2520

auccaacgua uuaacacccu caauuccuca gauacuaaag accugaugca gagagagauc     2580

aaggaagcua agaaacuacu gggugagaac uauaaugaaa aggaguucga aaacgauaau     2640

gaguucguga aaaaccacaa ggagaggcuc agccagcugg acgauuaccu ucgcuugcag     2700

aaaccuguua aguuugacac aaaguaugug gaacaagagg ccgauaaagu ccuggugucu     2760

uucaaggcag gcaaaaaggu agccacgauu accaaauuua aggcuacugg gaagaauaaa     2820

gacacaggag ugaucguuuu acggggcgcc cuccaugaac aguccgucua cgguaagaua     2880

aaagugauug agaaggacaa accacugaag uaccuuuucg aaaacaguaa aaagaucgug     2940

aaccccaaga ucaaaguccu gguugaggag agacuagcag aaaaugagaa caauagcaag     3000

cucucucugu ccaccuugaa aaagaacccg auuuuucuga augaagagaa aacagagauc     3060

uuagaaaagg cgucauguua uaacgaugcu acugugcuca aguacaaacu gcagagucuu     3120

aaggccagcc aggcagacga uauuguggac gauaggguaa aauuccuggu caaggagcga     3180

uuggcccaau uugggaauaa agaaaaggag gcuuucaagg acacccucug gcugaacgaa     3240

gagaaacaga uaccuaucau uacggugcgc cuauuugccc ggcccgaugc aaauaaccug     3300

caggcuauca agaaagacaa gaauggcaga gauauuggau ucguucuuac cggcaacaau     3360

caccacaucg ccauauauga ggacaaagau aacaagcucg ugcaacauuc gugcacauuu     3420

uggcacgcgg ucgaaaggaa gaaaaauaac gugccagccg uuaucaaaaa uuccucugac     3480

guguggaacc agcuguugga caaagagcug ccugagagcu ucuuaaacaa gcuccccauu     3540

gauaaacuga aacuuaaguu uucacugcag cagaaugaaa uguucguauu ggggcuccug     3600

caagaagacu ucgaggcugc aaucgagaac aaugauaaga cucuacugag uaagcaucuu     3660

uaccucgucu gguccauuac cgacggagau uacuuuuucc gucaccaccu ggaaacaaag     3720

aacacugagu ugaagaaaac cgaaggugcc aaggagucua aacgcuauuu ucgguuaagc     3780

acgaagggcc ugaucgaccu caauccagug aaggugagac ugaaccaucu uggggaaaua     3840

acaaaaauug gagaguauug a                                               3861


<210>  2918
<211>  3864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2918
augaagaccg ugcugggccu cgaccuuggg acaaacagca ucggaugggc ccugauugag       60

uuggaugaaa auaacauacc caucagaauu gucgcuaugg guuccaggau cauuccuuua      120

ucuacuucag acaaagagga guuccagcgg ggccugagua ucacgaagaa ucaaagccgc      180

accguuuccc gaacacagcg uaaaggguac gauagaaagc agcuaaagaa gucuuaucuc      240

aagaaaauac ugaacaagua caauaucuuu ccaucggacg agcuucugaa auugccgaug      300

cucgaucugu ggaagcugag gucagacagc gcaaacccca augaaaacau uagugccgag      360

cagcucggac ggaucuuaua uaugcugaau caaaaacgcg gcuacaaguc cgcgagaucu      420

gaggcuaacg ccgauaagaa agacacugau uauauucagg cagugaaggg uagguacgcc      480

cagcuuaaag accggaauca gaccaucggg caauauuucu acggagaauu gaacgaugcu      540

aaugagaaca auagcuacuu ucgcauaaag gaagagguau auccucgaga ggcauacaca      600

gaagaguucg acaccauuau caacgcccag aaagauaagc acgacuuucu gacugacgaa      660

gugauucauc agcuaagaaa cgagaucauc uauuuccaga ggaaacucaa gucccaaaag      720

ggccuggucu caauuugcga auuugagggc uucgagaaaa caguguuuga uaaggagaaa      780

aauaaggaca aaguuguguu cgucgggcca aagguggcuc ccaagaccag uccucuuuuu      840

cagcuguguc ggauauggga aguaguuaac acgaucacuu ugaaaauuaa gaauccagaa      900

ggaagcaaau acaagugggg ugagaaaauc cccgauguga aggaaaagga gauacucgcc      960

gacuaucugu ucaaaaacga gucuuuaucc uuuaccaagc ugcucgaaau ucuuaaacug     1020

aauaagaaca augucuacuu caacaaacag aucuugaagg gcaucaaggg gaaugagaca     1080

uauucaauuc uacacagcau ccugggagau agugaacucc ugaacuucga cgugucuauu     1140

auaccuucca aucauacuag caugcuuauc gauaaaaaga ccggcgagau ucuggaggaa     1200

cgugcggguu uggagcucga cacaucgcug gaaaaagagc cauuaaacca acuguggcac     1260

accaucuacu cuaucaagga ucuagacgaa ugcaaauccg cacucauuaa gcgcuuuggg     1320

uucgaugacg agacggccga caaacuguca agaauagauu uuaauaagca ggcuuucgga     1380

aacaagagua auaaaagcau gaggaagauc cuuccguuuu ugauggaggg cuacgacuau     1440

ucccagucuu guagccuggc cggcuacaac cauucaaaca guuucacuaa agaugaacgg     1500

gagcagcaaa uuacacucga caagcuggaa cuucugccca aaaauuccuu gcgacagccu     1560

aucguggaga agauucucaa ccagaugauc aaugucguua acgcaauacu ggaacgcuau     1620

gggaagccaa gcgagaucag aguggaguua gcuagggaac ugaaacaguc uaaggaugag     1680

cggaaugaca ccgaucuaca aaacaguaaa aauaagaaac ucaacgaaga gauuggaaag     1740

cgccuggccg aacuugguuu gccccugacu aagagauaca uccagaaaua uaaguuuauu     1800

uucccuucac ucagcaaaaa ucugaaggac gcgcacguaa acaaucagug caucuacugu     1860

ggcgagacau uuaacuuaac cgaggcccug uccggggaug cauucgacgu ggaucacaua     1920

gucccaaaag cucuuuuguu ugacgauucu cagacgaaua aggugcucgu ucauaggaag     1980

ugcaacucga ccaaaacaaa ucaaacugcc uaugacuaca uugcuaccaa gggagacgaa     2040

caggugcgug ucuacagcga gcggguggau gacugguuca aacgaggcau cauuuccuau     2100

ucaaagaugc agagacugaa aguaagucac aaggaauacc uagagaggaa aaagcugggu     2160

aaggaaacag agacugauaa aaagcucugg gagaacuuua ucgaccgcca gcugcgggau     2220

acccaauaua uaucuagaaa aagcagggaa auccuucaga agguuuguaa caauguggcc     2280

acaaccgaag ggggcgucac ugcaaaauug cgcaaccugu ggggauggga cgauguguua     2340

augaaucucc agaugcccaa guacaaggaa cugggccaaa cggucgugaa agaguggaca     2400

uccgagcaug ggaagcgaaa acaccagaag gaagagauug aaaacuggac caaacgggac     2460

gaucaccguc aucacgccau cgacgcucuu guuguggcgu gcacuaagca gggauucauu     2520

cagagaauca auacccugaa cucaagcgag acaaaggauu ugaugcaaca ggaaauaaaa     2580

gaggcaaaga aacuccuggg ugagaauuau aacgaaaagg aguucgaaaa uaacaaugag     2640

ucugucaaaa accauaagga aaggcuaucc cagcuggacg auuaccuccg ccugcagaag     2700

ccgguaaaau uugacaccaa guauguggag caagaggccg acaaaguucu ugugaguuuc     2760

aagagcggca aaaaggucgc uacuauuaca aaguuuaaag ccacggggaa gaauaaagau     2820

accggaguga ucguaccucg gggcgcauug cacgaacaga acguguacgg uaagaucaaa     2880

guuauugaga aggacaaauc ucugaaguac uuauucgaaa auuccgagaa gaucgucaac     2940

cccaaaauua agaacauagu ggaaaucauu cucucagaga augagaacaa uaguaaaaug     3000

gcccugagca cucuuaagaa aaacccaauc uuucugaaug aagagaagac agaucuacuc     3060

gaaaaggcuu cguguuauga guacgugacc gucagaaaau auccucugaa cacaauuaag     3120

gccucccagg cagacgauau cguugacgag agggugaaau ucuuggugaa ggaacgacug     3180

acucaguuug ggaauaaaga gaaggaagcg uucaaggaua ccuuaugguu uaacgaggaa     3240

aaacaaauac ccaucauuac gguacgccuc uucgcucggc cagacgccaa uaaccugcag     3300

gcaaucaaga aagaugaguc uggcaaggag auuggauuug ucgugauggg caauaaccau     3360

cacaucgcca uauacgaaga caaagauggg aaacuuguuc agcacagcug caccuucugg     3420

caugcugugg agagaaagaa auauaauauu ucaacaguca ucaagaacag uuccgacgcc     3480

uggaaucaau uucuggacaa cgaauugccc gagccuuucc ucaauaaacu gccagcagau     3540

aaccuaaacc ugaaguucuc ucuucagcag aaugaaaugu uuauccucgg acugagccag     3600

gaggaguucg acaaggcuuu gaaguccaac gauaaaucac uguuaagcaa gcaccucuac     3660

cuggugugga guauuucuga aaauaacuau ugguuuaggc aucaccuuga gacuaaaaau     3720

uccgaccuga aaaagaccaa cgguguuaaa gaaagcaagc guuuguacaa uauacgcucg     3780

cucggcucac uguucucucu aaacccgauc aaaauucggc ugaaucaccu uggggagauc     3840

acaaagauug gagaauacag uuga                                            3864


<210>  2919
<211>  3867
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2919
augagacugu ucgaggaagg ccacaccgcc gaggacaggc ggcucaagcg cacagcucga       60

cguagaauca gcaggcggcg caacagacuu agguaccugc aggcauuuuu cgaagaggcc      120

augacugauu uggacgaaaa uuucuucgcg cgguuacaag aguccuuucu ggugcccgag      180

gauaaaaagu ggcaucgcca cccuauuuuc gcuaaacuag aagacgaggu cgccuaucau      240

gaaacguacc caaccauaua ucaccuccga aagaaacugg cagauucuuc agagcaggcc      300

gaccuuagac ugaucuacuu ggcucucgca cauauuguua aguauagggg gcacuuucug      360

aucgagggaa agcugaguac agaaaacacu agcgugaaag aucaguucca gcaauuuaug      420

guaauuuaca aucagaccuu cgugaacggu gagucccggc ucgucucugc cccguuaccc      480

gaaucggugc ugaucgagga agaacuuaca gagaaggcuu cacguaccaa aaagagcgaa      540

aaaguuuugc agcaguuucc ucaagagaag gccaauggcc uguucgggca guuucuaaag      600

cucauggugg gaaacaaagc ggacuucaag aaagucuucg gccuggaaga ggaagcaaag      660

auaacuuaug ccagugaguc cuacgaggaa gaucuugagg guauccuggc uaaagugggg      720

gacgaauacu cugauguauu uuuggccgca aagaauguuu augacgcugu ggagcucagc      780

acaauucugg ccgauuccga caaaaaguca cacgcgaagu uaaguagcuc uaugaucguc      840

cgcuucaccg aacaucagga ggaccugaaa aaguuuaaaa gauucauuag ggagaacugc      900

ccagaugaau acgacaaucu cuuuaagaac gagcagaaag auggauaugc cggcuacauc      960

gcacacgcug gcaagguguc ccaacuuaag uucuaucagu acgugaaaaa gauaauucag     1020

gacaucgccg gggcugaaua uuuucuggag aaaauugccc aagagaauuu cuugcggaag     1080

cagcgaacgu uugauaacgg agucaucccc caucagaucc accuagcaga acugcaggcc     1140

auuauacacc gccaagcugc guacuacccu uuccucaaag agaaucagga aaagaucgag     1200

cagcugguua cuuuuaggau uccauauuac gugggucccc uuucaaaggg cgacgcaagc     1260

accuucgccu ggcugaaaag acagagugag gagccuaucc ggccauggaa cuugcaagag     1320

acaguagauc ucgaccaguc ugcuacugcc uuuauagaac gcaugaccaa uuucgauaca     1380

uaucugccgu ccgagaaggu guuacccaaa cauagccugc uauacgaaaa guucaugguc     1440

uuuaacgagc ucaccaaaau uucguauacg gacgauagag ggaucaaggc aaacuucucu     1500

ggaaaggaaa aagagaagau cuuugacuac cuguucaaaa cuaggcguaa ggugaaaaag     1560

aaagacauua uccaguuuua ucggaaugag uacaacacag aaauuguuac ccuuuccggc     1620

uuggaggaag aucaguucaa ugccucauuu aguacuuacc aagaccugcu caaguguggu     1680

cugacacgag cugagcuuga ucacccugac aacgccgaaa agcuggagga uauaaucaaa     1740

auuuugacca ucuucgagga cagacagagg auccgcacgc agcucagcac cuuuaagggg     1800

caguucuccg cagaagugcu gaaaaaguua gagcggaaac auuauacagg auggggcaga     1860

cugucuaaga agcuaauuaa uggcauauac gauaaagaaa gcgggaagac uauccucgac     1920

uaucugguca aagaugacgg agugucaaag cacuacaaca ggaauuuuau gcaacuuauu     1980

aacgauaguc aguuguccuu caaaaaugcg auccagaagg cucaaagcuc ugagcaugag     2040

gaaacccugu cagagacagu aaacgaacuc gccgguaguc cagcaauuaa gaaaggcauc     2100

uaucagagcc ugaagauagu ugacgaguua guggccauca ugggguacgc ucccaaacgc     2160

auugucgugg aaauggcccg agagaaucag acuaccucca caggaaagcg gcguucuauc     2220

cagagacuga aaauugucga gaaggcaaug gcugaaaucg gcucgaaccu uuugaaggag     2280

caaccuacca cuaaugaaca gcucagggau acgcgccugu uucuauauua caugcagaac     2340

gguaaagaca uguacacagg ggacgagcug agccuccacc ggcuguccca cuaugauaua     2400

gaccauauua ucccacaguc auucaugaag gaugacaguc uugauaauuu ggugcugguu     2460

ggcucuaccg aaaacagagg aaaaagcgac gaugugcccu ccaaggaggu agucaaagac     2520

augaaggccu acugggagaa auuauaugcg gccggccuca uuucacaaag gaaguuccag     2580

cgacugacua agggggaaca gggaggucuu acccuggagg auaaagcuca cuuuauccag     2640

cgccaauugg uggaaacacg gcagauaacc aagaacguug caggcauccu cgaccagaga     2700

uacaaugcca aaagcaagga gaaaaaggug caaauuauca cucugaaggc uucucuaaca     2760

ucccaguuca ggaguauuuu ugggcuguau aaaguccgug aagugaacga uuaccaucac     2820

ggacaggacg ccuaucucaa uugcguagug gcaacgaccc ugcuuaaggu uuacccgaac     2880

uuggcgccug aguucgucua cggcgaguau cccaaauuuc agacuuucaa ggaaaauaaa     2940

gccacagcua aggccaucau auacaccaac cuguuacgcu uuuucacaga ggaugaacca     3000

cgguuuacua aggacgguga gauucucugg agcaauucuu aucugaaaac caucaagaaa     3060

gagcuuaacu accaccaaau gaauaucgug aagaaagugg aaguccagaa agggggcuuc     3120

uccaaggagu caauuaaacc uaagggaccc aguaacaaac ugaucccagu uaagaauggc     3180

cuagaccccc agaaauaugg gggauuugau agcccugugg uggcauacac gguacucuuc     3240

acccaugaaa aggguaagaa accacugauu aagcaggaga uauugggcau cacaauuaug     3300

gaaaagacua gauucgagca aaacccgauc cuguuuuuag aggaaaaggg guuccucagg     3360

ccccgagucc ugaugaaacu uccuaaguau acccuguacg aguuuccaga aggacggcgc     3420

agauugcucg cuucggccaa agaggcacag aagggcaauc agauggugcu gcccgaacac     3480

cuacugacac uucucuacca ugccaagcag ugucuguugc cuaaccaauc cgagucucug     3540

gcuuauguug agcagcacca gccagaauuu caggagauuu uagaaagggu ggucgacuuu     3600

gcggaggugc auacucucgc caaaagcaag guucaacaga ucgugaaacu guucgaagca     3660

aaucagaccg cugauguaaa ggagauagcc gccucauuua uccaacuuau gcaguucaac     3720

gcuaugggug cacccaguac guuuaaauuc uuucagaagg acauugagcg ggcccgauac     3780

acauccauca aggaaauuuu cgaugcgacc aucauauauc agucuccgac ugggcuguac     3840

gagacaagac gcaaagucgu ggacuga                                         3867


<210>  2920
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2920
auguggggcg ugagccuguu cgaggccggg aagaccgcug cagaaagaag gggauaccgg       60

uccacacgcc gacgucucaa ccacagaaaa uuuaggcuuc ggcuguugga ggacauguuc      120

gaaaaggaga ucuuaucuaa agaucccuca uuuuucauuc gccugaagga agccuuucua      180

aguccuaaag acgagcagaa gcaauucaag gguaauauac ucuuuaacga uaaagacuau      240

acugaugcgg acuacuauga gcaguacaag acgaucuauc aucugagaua cgaucuuauu      300

agccagcaca ggcaguucga cauccgggaa gucuaucugg cuauucauca cuugaucaaa      360

uaccgcggcc auuuuauaua cgaggaucaa accuucacaa cugacgggaa ucagcuccag      420

caccacauca aggccauuau caccaugauu aacuccacac ugaaucgaau cauaauucca      480

gaaaccaucg auauuaacgu uuuugagaaa auccugcucg acagaaugau gaauaggucu      540

ucgaagguga aguucuuaau cgagcugacu ggagaagaua aacaggacaa gccgcuuuug      600

aaagagcugu uuaaccuaau uguaggccuc aaggcaaaac ccgccucaau auucgaacaa      660

gagaaucugg cuacaaucgu ggaaaccaug aacaugagca cggagcaggu ccagcuugac      720

cuguugacuc ucgcagaugu gcuggccgac gaggaauaug auuuacugcu caccgcucag      780

aagauuuaca gugccaucau acuugacgag uccauggaug guuaugaaua cuucgcggag      840

gcaaagaagg aaucuuaucg gaagcaucaa gaggagcugg uuuuggugaa aaagaugcua      900

aaaagcaaug ccauuacaaa cgacgaacgu gcuaaguuug aguacuucua uacugauuac      960

aucggggcca aauccucaaa uuacgaagag aguaagaaca ucaagaaagg acugagcgca     1020

gcuuauggca aguacucuaa agaagaacgc cucuuuaagc acauugagcu gcuucuggcc     1080

aaagaaaaug ucuugaccac aguggagcau gcgcuccugg aaaagaacau caccuucgcc     1140

uccuuacugc cucuacagag aucaagcagg aacgcaguaa uuccauauca gguucacgag     1200

aaggagcucg uggcuauacu ggaaaaucaa gccacguacu aucccuuucu uuuggagcag     1260

aaagacaaca uccacaagcu gcucacuuuc cggauuccuu acuaugucgg cccacuggcu     1320

gaucagaaag acagugaauu ugccuggaug gugcgaaagc aggcagggaa aaucacaccc     1380

uucaauuuug aggaaauggu ggauaucgac gccucuuccg aggcuuucau uaagcgcaug     1440

accaacaagu gcacuuaccu uauacaugag gacgucaucc cuaaacacag cuuuucguac     1500

gcgaaguucg aaguucugaa ugaguugaac aaaauuagac ucgauggaaa gccaaucgac     1560

auuccgcuga aaaagaggau cuuugaaggu uuauuccugg agaaaacaaa ggugacccaa     1620

acgucucuaa agaaauggcu cgcagaacau gagcacauga ccguauccgu gguccagggc     1680

acacagaagg agacugaguu cgccaccuca cugcaggcuu uucaucgguu cgugaaaaua     1740

uuugaucgcg agacaguuag uaaucccgcc aacgaagaga uguucgaaaa gaucauuuau     1800

uggagcacug uguuugagga caaaaagauc augagaagga aacuuuccga guacccucaa     1860

uugaccgaac agcagcaggu ccaacuggca caggugcguu uccgggggug gggacgacuc     1920

ucucagagac ugauuaauag gaucaaaaca ccaguaagcg gcgaugagga ccacaaguua     1980

ucaauaaacg aaauucugug gcagaccaau gagaacuuua ugcaaaucau ucgcaauaaa     2040

gauuaucuuu ucaagaaaau cauagaggaa caguuugaga acgaaacugc cuugcucaau     2100

aagcagcgga ucgacgagcu ggcugccagu cccgcaaaca agaaagguau uuggcaagcg     2160

aucaagauug uuaaagaacu agagaaggug cugcagcagc cugcugagaa caucuucaua     2220

gaauuugcca gauccgauga ggaaagcaaa aggucuacgc cacgcgacaa guucauugag     2280

aaggcauacg cccagcucaa aaaggaaaca gauaccuuua aucuggagca ccuuaaagag     2340

uugaagcaac gaucaaaaca gcugaguagc cagcgguuau uccucuauuu cauccagaac     2400

gggaagugua uguacuccgg agaacaucug gacaucgagc gucuugauuc uuaugaaguc     2460

gaccacauuc ugccccaauc guacaucaaa gacaauagca uugagaacuu ggcucucgug     2520

aagaaggucg aaaaucagag gaaaaaggau ucccugcuac ugaacucaag uauaaucaau     2580

cagaacuacu cuagauggga gcagcucaaa aaugccggcc ugauuggcga gaagaaauuu     2640

cgcaaccuua cucggaccaa gaucacagac agagauaagg aaggguucau ugcaaggcaa     2700

uugguggaga cccgacagau cacuaaacau guuacacagc uguuacaaca ggaauauaag     2760

gacacgacca aaguguuugc uauaaaggcc acucucguaa gcggacugcg ccggaaauuc     2820

gaguuuauca agaauagaaa cgucaaugau uaccaccacg cgcaggacgc cuuccuugug     2880

gcuuuucugg guacaaacau uaccuccaau uauccgaaga ucgagaugga auaccuauuc     2940

aaaggcuauc agcauuaccu caacgagguu gggaagucag cagccaaacc uaaguuuaca     3000

uucauugugg aaaaccugag caaacaacag uauaauucua cuaccggaga ggucaagugg     3060

aaccccgaag uggauaucgc uaaguugaaa aggauacuga auuuuaagca gugcaacauu     3120

guacguaaag uggaggagca guccggcgcc uuauucaagg aaacgaucua cccaguugag     3180

gaaaguagcu cuaaaaccau cccucucaag aagcaccugg acacagcaau uuacgguggg     3240

uauacugcgg ucaauuacgc cuccuaugcu cuuauacaau ucaaaaaggg ccgcaaacug     3300

aagcgggaga ucauuggaau ccccuuggcc gugcagacca gaauugauaa cucagaaaca     3360

agucuccagg cauacaucgc ugagcagaua aaaagcgagg uggaaauccu gaauggcagg     3420

auucuaaagu aucaacugau cucgaacaau gggaaccgac uuuacauugc cggaccaucc     3480

gagcggcaua augcacgcca gcucaucguc ucugacgaag ccgcuaaagu uauauggcug     3540

auuagcacua agcaggcgga ugaggccaug uuuuugaagu auuacagacu ggaacacuua     3600

gaggcagugu ucgaggaacu caucaggaaa caggcugccg acuaccaaau cuuugagaag     3660

cugauuaaaa agaucgaagu gaacaaagua uauuucuacu cauguaccua uaaugagaag     3720

gucaagguga uagaggaacu ucugaaaauu acgcaggcca acgcuacaaa uggugauuug     3780

aagcuccuga aaaugaguaa ccgggagggc cgacuagggu ccguuucugu ggcacuggac     3840

uuuaagauca uuaaccagag cgucaccgga cuuuaccaau ccaucgaaga cuauaauaac     3900

uga                                                                   3903


<210>  2921
<211>  3930
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2921
augggcagcg ugggguucgc cggaaucgac aaccaguaca aucuggucag aaccaagggu       60

aaaaacguua uuggcgugag gcucuuugau gaggcugacu ccgcagccga acggcgcggg      120

caccgaacaa aucguagaag gcuucaacgg cgcagaugga ggcugcgguu guuagaugac      180

auauucgcga agccccugca ggcuguagau ccuaacuuuc uagcccgccu caaauauucu      240

uacgugaaua agaaagacca gggacagcaa gaucauuauu acggcgguua ugucuucggg      300

ucaacugcag ccgaccaggc uuaccaccag gcauauccaa cgaucuacca ucugcgaaag      360

agacuuaugg aggaugacca gaagcacgau cugagggaag uguacuuggc cauucaucac      420

aucguuaaau aucggggaaa cuuucucaau ccgcaaagua gccuggacau ugaucagcag      480

uucgacguga ccgacuuugc ucaggcccug gcgcguuucg cagaucacca agcccucucc      540

ugggcuuuag aggcccccau ccgcuuucug gaagcagagc uugcuacagg cuugucuaac      600

ucggccagag ucgacgcggc cauagaggca uucucauuug auacuaaggu ggacagggcu      660

gccaucaaag aaaugcugaa gggccuaagc gggaaucaga uugauuucac caaacucuuu      720

guaaacguug acagugcuga uugggaccag gaggaacgga agcaauggaa gaugaaacug      780

uccgaggagg auuucgacga acaggcccuu ccuauccugg agcgauuguc ucaggaugaa      840

acagaguucu uucucgcaau uaagcgcgcc uacgacggaa ucgcucugau gagauucuua      900

ggugacgaac agagccuguc cucagcgaug auaaaagcau augaggauca uaggcgggac      960

cucaccuuuc uuaagacuca agugcgcaca ccacagaaua gacaggcccu gagugagggc     1020

uacaccaacu auuugagcgu cgaugacaaa aagcacaaaa gaggggcuaa ggaacuagcc     1080

cagcugauug aggcaucuga ugccuccgaa caagacaagg cuacgaugcu cgaucguauc     1140

gccaaugacc aguucgcacc caaacagcgg acuaaggcga acggacugau uccuuaccag     1200

cuucaucugg cugaguugaa aaagauccuc gccaaacaag gccaguauua cccauuucug     1260

uuagauaccu ucgcaaagca gggucaguca gugaauaaga ucgaggagcu ggugcaauuu     1320

cgaguccccu acuauguugg gccuauggug ccaaaaagcg agacagccgg aaacgcugaa     1380

aaccacuggg uagagaagaa ugacggccag acuaaaguga gugucacccc guggaacuuc     1440

gaucaggugu uuaauagaga cagggccgca aagucuuuca uugaucgccu aacaggcacc     1500

gacacguacc ucauagggga acccacucug ccucggcacu cccuuacaua ugagaccuuu     1560

acuguuuuga acgaacugaa uaacaucaga auugacggaa aaaggcuccc aguggagaca     1620

aagcaagcua ucgucgagga ucuguucaag aaauaccgcc uugugaccaa gaaacgacug     1680

caggacuauu uugccagcuu cgguaagcgg gaaguagagu ugacgggccu cgcggaugaa     1740

ucgcguuuca ccucuucccu gacaucauac caugacuuac aggggcugcu aggaacugau     1800

uuuauaacca auccccagaa ccacagucuc cuggagaaaa uuguugagau ccaaacagug     1860

uucgaagaca gcgauaucgc cgagagagaa cuuggcaagu ugggucugga gcagaagcuc     1920

auuccuaggc uggcuaaaaa gcauuauacu ggguggggca auuuaucccg caaacugcuu     1980

gacaccucuu uuauccacga uccagaacgg cccgaggagc cggucagcau uauggacuug     2040

cucuacacaa ccaacaagaa uuucauggaa auacugcaug auucagagua cggaguggaa     2100

gaguggcuaa aaagucagaa caugaucgac gaucagaagg acauucaaau gagaaucgac     2160

gaacugacua cguccccugc aaauaaaagg ggcaucaagc aggccuuuaa cguccucgau     2220

gacauuacac aggcuauggg ggaggagccc gccuaugugu accuggaguu cgcacgagaa     2280

aagcaggcga gccgccggac cguuucuagg aaaaagaggc uugaaacuuu guauaaaaau     2340

gccgcucuga agaccgaguu uaaagccaua aaggaagcau uagcugagga gucagaugac     2400

cguaugcaag augaccgccu cuaccuguau uacgcccagc uuggacggga uauguauaca     2460

ggucagagua ucagcauuga ccaacugucc ucucacuacg auaucgacca cauugugcca     2520

agagcauuca ucaaggauga cucguuggaa aacaaaguac ucgucaacag gaccgacaau     2580

gcccgaaaga cugauagcgc uacauuuacg gcggacguga aagccaaggc auucccucug     2640

uggcagcagc uaaagaagcu gggccucaua uccgcuaaga aauuucggcu gcuuacccgc     2700

acuggggauu ucacagagau ggaaagagag agguuuaucg cccggcaguu gguugagacc     2760

cgacaaauua ucaagaacgu ggccgcucug auugaaggac auuucucaca gacacaggca     2820

gucgccauca gagcggaggu gacuggcgaa uuacgccagc ucacccaaau uaaaaaggac     2880

agggauauaa augacuacca ccaugcucag gaugcccugc uuguagcaac ggccgguacc     2940

uaucugcacc gucacuuccc caaacgggac gcuagauuua ucuacaacga guucgauuau     3000

uacacacagc auuggcuaaa gaaucagggg gaaaacaggc gccgacaccc auauaguuuu     3060

gugguuggca cuaugucuaa gggaaaugag gacuggaccc ccgauaaccu caauuaccug     3120

agaaaaguca ugcaauauaa gacaauguug augacucgga aaccuguggg cccagagggg     3180

gcacuguaca aggaaaccuu aauugccgcg gacccgaaaa agaggcucgu gggagcuagc     3240

aaagagcgcc aggaucccac gaucuacggu ggcuauacaa aggaauccuc agccuacaug     3300

agccuggucc gugcaggggg aaagaaccag cuuguuaaaa uaccugugag aaucgccaau     3360

gagauucauu cuggccagcg gaagcuggac gacuaugugc aagcuaaagu aaagaaauuc     3420

gaaaggaucu ugcucccaaa gauuucccug ggucagcuag ucgaggauga ggggcagcgc     3480

uuuuaccugg ccaccaacga aaugaagcac aaugcaaaac aacuuuggcu cgaccagaag     3540

gugguuacua cauauaaacg acugaccgcu gagagucccg uggaagauuu cuugacuguc     3600

uuugacgccc ugaccagcuc ugcgacaauc caucacuuca aguuuuacca gagagauuua     3660

gagcuccugc gggacaacag ggccggcuuc caggaucuug caaaagcuac gcaacugaag     3720

guguugaagg acguucucua ugaacugcac gauaaugccg gauggcgcga cccuauaaaa     3780

caguacuuua aggagauugg ccuaaaagug agaaugugga ccaagcugca gaaagaaggg     3840

ggaaucaaac uuacugauca ggcugaacuc aucuaccaau ccccaucagg ccuguucgag     3900

aagaggcggc guguacagga cuuguuauga                                      3930


<210>  2922
<211>  3939
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2922
augcagaacg gcuuccuggg gaucaccgug agcuccgagc aagucggaug ggccguuaca       60

aaucccaagu acgaacucga gagagcuucu aggaaagacc uuuggggugu gcggcuguuu      120

gauaaggcag aaacugccga ggaccgccga auguuccgua cgaacagaag guugaaucag      180

cggaaaaaga accgcauuca cuauuuaaga gauauauuuc augaagaggu aaaucagaaa      240

gacccuaacu ucuuucagca acuggaugag ucaaauuucu gcgaagacga uaggaccgug      300

gaguuuaacu ucgacacaaa ucuauacaag aaccaguuuc caacugucua ucaccuccgg      360

aaguaccuga uggaaaccaa agauaagccg gacauccgcc uuguguaucu ggcguucagu      420

aaauuuauga agaaucgagg ccauuucuug uacaaaggga accucggaga gguuauggau      480

uuugagaaua gcaugaaggg cuucugugaa ucccuggaga aguucaacau ugacuuuccc      540

acacugucug augaacaggu gaaagagguc agagacaucc ucugcgacca caagauugcu      600

aaaaccguga agaaaaagaa uaucauaacu aucacaaaag uaaagucgaa gaccgccaaa      660

gcauggauug guuuauucug ugggugcuca guuccuguga agguccuguu ucaggauauc      720

gacgaagaga uugugacgga uccagagaaa aucagcuucg aagacgccag uuaugaugac      780

uacauagcua acauugagaa gggagugggc aucuacuaug aagcaauugu cuccgccaaa      840

augcuuuuug auuggucuau caugaacgag aucuugggcg accaucaacu gcuaagcgau      900

gcuaugauug ccgaauacaa uaagcaccac gacgaucuca agaggcugca gaaaauaauc      960

aaggggacug gaucccggga gcuuuaucag gacauuuuca ucaacgacgu uucagguaau     1020

uacguguguu auguaggcca ugcgaaaacc augaguagcg cagaucagaa gcaauuuuac     1080

acauuccuga aaaaccguuu gaagaaugug aacgggauau cuuccgagga cgccgaaugg     1140

auugauacug agaucaagaa uggaacccuc cugcccaaac agacaaagcg cgacaacuca     1200

gucaucccuc accaguuaca acugagagaa uuugagcuca uucuugauaa uaugcaggaa     1260

auguauccau uccugaaaga gaacagggag aaguugcuaa aaaucuuuaa uuucgugauu     1320

cccuacuacg uuggcccucu gaagggugug guccggaagg gggaaagcac caacuggaug     1380

gugccgaaaa aggacggagu aauacaucca uggaauuuug augagauggu ugacaaagaa     1440

gcuagugccg agugcuucau cucucgaaug acgggcaacu guuccuaucu cuucaaugag     1500

aaggugcugc ccaaaaacag ccuucuguac gaaacuuuug aggucuugaa cgaacucaau     1560

ccucugaaga uuaacggcga gccaaucucg guggaauuaa aacagcgcau cuaugagcag     1620

cuguuccuaa cagggaagaa ggucaccaaa aagucucuca cuaaauaccu gauuaagaau     1680

ggauaugaug aggacauaga acuuuccggu aucgauaacg aguuucacuc aaauuugaaa     1740

agucacauug acuucgaaga uuacgacaac cugagcgacg aggaagugga gagaaucauu     1800

cucaggauca caguuuuuga ggauaagcaa cugcuuaagg acuaucugaa ucgggaguuc     1860

gugaaauugu ccgaagauga acgcaagcag auaugcucuc ucagcuacaa aggcuggggg     1920

aaccugucag agauguuacu gaauggaauc accguaacgg acaguaacgg cgucgaagug     1980

uccguuaugg auaugcuaug gaauaccaac cucaaucuga ugcagauucu uagcaagaaa     2040

uacgguuaua aggcagagau cgagagauac aacaaggaac augagaaaaa uauuuauaac     2100

agggaagacu ugauggauua ccugaacaua cccccugcuc agcgucggaa ggugaaucaa     2160

cucaucacaa uugucaaauc ucugaagaaa cgaugugggg ugccaaacaa gaucuuuuuc     2220

aagguaucaa gagagcacca ggacgauccc aaaaggacca guagccgcaa ggagcaguua     2280

aaauaucugu acaagucccu uaaaucugaa gacgagaagc auuugaugaa agaacucgau     2340

gagcugaaug gccacgaacu aucgaacgac aagguguauc uguacuuucu ccagaaggga     2400

cggugcauuu acagcggcaa aaagcugaau cuuuccagau ugaggaaauc aaacuaucaa     2460

aaugauaucg acuacauaua uccgcugagu gccguuaacg accgcucuau gaauaacaag     2520

gucuuaacug ggauccagga gaaucgagcg gauaaauaca cauauuuccc uguggacccc     2580

gagauucaga agaagaugaa aggauuuugg auggaacucg ugcugcaggg uuucaugagc     2640

aaggagaaau acuuucggcu uucccgugaa aacgauuucu cagagaauga acuggucagc     2700

uucaucgaga gagagauuuc ugacaaccaa caguccggca ggaugaucgc caguguuuug     2760

caguauuacu uuccagaaag caagauagug uucgugaaag agaagcucau uucuuccuuu     2820

aagcgcgauu uccaucugau cucaaguuac gggcacaauc accuacaagc agcuaaagac     2880

gccuauauca cuauuguagu cggaaacgug uaccauacca aguuuacaau ggauccugcu     2940

aucuauuuca aaaaccacaa gcggaaagac uacgaucuga auagacucuu ucuggaaaac     3000

auuagcaggg acggccagau agccugggag ucgggucccu auggguccau ccagaccguu     3060

cgaaaggaau acgcacagaa ucauauugcc gugacuaagc gcgucgugga gguuaaaggc     3120

ggacuuuuca agcaaaugcc auugaaaaag ggccacgggg aguauccccu gaaaacgaac     3180

gauccucggu uuggaaauau cgcucaguac gguggcuaca caaacgugac cgggucuuau     3240

uucguauuag ucgaaagcau ggagaaggga aagaaaagaa uuucacucga auacgugcca     3300

guguaucugc acgagaggcu ugaggacgac ccgggccaua agcugcuaaa agaauaccuc     3360

gucgaucacc guaagcugaa ucaucccaaa aucuugcugg cgaagguucg caaaaacagu     3420

uuacucaaga uagacgguuu uuauuaccgg cugaauggga gauccggcaa cgcacuuauc     3480

cugacuaaug ccguggaguu gauuauggau gacuggcaga ccaagacagc uaacaaaauc     3540

ucuggauaua ugaagaggcg agccauugau aaaaaggcac ggguauacca gaaugaguuc     3600

cacauccaag aacucgaaca gcuguacgac uucuaucuag auaaacugga gaacggcgug     3660

uacaagaauc gcaagaacaa ucaggccgag cuuauacaua acgaaaaaga gcaguuuaug     3720

gaacucaaga ccgaggacca auguguccug uugacugaaa uuaaaaagcu guucgugugc     3780

agcccuaugc aggcugauuu aacacucauc gggggaucca aacacacggg caugaucgcc     3840

augucaagca acguuaccaa ggcagacuuu aguauuauag cggaggaucc acugggucuu     3900

agaaauaagg ugaucuauuc ucacaugggg gagaaauga                            3939


<210>  2923
<211>  3939
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2923
augcagaacg gcuuccuggg gaucaccgug agcuccgagc aagucggaug ggccguuaca       60

aaucccaagu acgaacucga gagagcuucu aggaaagacc uuuggggugu gcggcuguuu      120

gauaaggcag aaacugccga ggaccgccga auguuccgua cgaacagaag guugaaucag      180

cggaaaaaga accgcauuca cuauuuaaga gauauauuuc augaagaggu aaaucagaaa      240

gacccuaacu ucuuucagca acuggaugag ucaaauuucu gcgaagacga uaggaccgug      300

gaguuuaacu ucgacacaaa ucuauacaag aaccaguuuc caacugucua ucaccuccgg      360

aaguaccuga uggaaaccaa agauaagccg gacauccgcc uuguguaucu ggcguucagu      420

aaauuuauga agaaucgagg ccauuucuug uacaaaggga accucggaga gguuauggau      480

uuugagaaua gcaugaaggg cuucugugaa ucccuggaga aguucaacau ugacuuuccc      540

acacugucug augaacaggu gaaagagguc agagacaucc ucugcgacca caagauugcu      600

aaaaccguga agaaaaagaa uaucauaacu aucacaaaag uaaagucgaa gaccgccaaa      660

gcauggauug guuuauucug ugggugcuca guuccuguga agguccuguu ucaggauauc      720

gacgaagaga uugugacgga uccagagaaa aucagcuucg aagacgccag uuaugaugac      780

uacauagcua acauugagaa gggagugggc aucuacuaug aagcaauugu cuccgccaaa      840

augcuuuuug auuggucuau cuugaacgag auccugggcg accaucaacu acucagcgau      900

gcuaugauug ccgaauacaa uaagcaccac gacgaucuga agaggcuuca gaaaauaauc      960

aaggggacug gaucccggga gcuguaucag gacauuuuca ucaacgacgu uucagguaau     1020

uacguguguu auguaggcca ugcgaaaacc augaguagcg cagaucagaa gcaauuuuac     1080

acauucuuga aaaaccgucu caagaaugug aacgggauau cuuccgagga cgccgaaugg     1140

auugauacug agaucaagaa uggaacccug uuacccaaac agacaaagcg cgacaacuca     1200

gucaucccuc accagcugca acucagagaa uuugagcuua uucuggauaa uaugcaggaa     1260

auguauccau ucuugaaaga gaacagggag aagcuacuga aaaucuuuaa uuucgugauu     1320

cccuacuacg uuggcccucu caagggugug guccggaagg gggaaagcac caacuggaug     1380

gugccaaaga aggacggagu aauacauccg uggaauuuug augagauggu ugacaaagaa     1440

gcuagugccg agugcuucau cucucgaaug acgggcaacu guuccuaucu guucaaugag     1500

aaggugcuuc ccaaaaacag ccuguuguac gaaacuuuug agguccucaa cgaacugaau     1560

ccuuuaaaga uuaacggcga gucgaucucu guggaacuga aacagcgcau cuaugagcag     1620

cuauuccuca cagggaagaa ggucaccaaa aagucccuga cuaaauaccu uauuaagaau     1680

ggauaugaua aagacauaga guugucaggu aucgauaacg aauuucacag uaauuuaaag     1740

agccacauug acuucgagga uuacgacaac cucuccgacg aagaggugga acaaaucauu     1800

cugagaauca caguuuuuga ggauaagcag cuucugaaag acuauuugaa uagggaguuc     1860

gugaagcucu cugaagauga gcggaaacag auaugcagcc ugucauacaa gggcuggggg     1920

aacuuaagug aaaugcugcu aaauggaauc accguaacgg acuccaacgg cgucgaggug     1980

agcguuaugg auaugcucug gaauaccaac cugaaucuua ugcagauuuu gucuaaaaag     2040

uacgguuaua aggcagaaau cgagcauuac aacaaagagc acgaaaagac aauuuauaau     2100

cgcgaggacc ugauggauua ccucaacauc ccacccgcuc aaagaaggaa agugaaccag     2160

cugauaacua uugucaaguc acugaaaaag accuaugggg ugccuaauaa gaucuuuuuc     2220

aaaguaaguc gugaacauca ggacgaucca aagcggacaa gcucccgaaa agagcagcug     2280

aaguaccuuu auaaaucuuu gaagucggag gacgaaaaac accucaugaa ggagcuggau     2340

gaacuaaacg gccaugagcu gagcaaugac aagguguacc ucuacuuucu gcaaaaagga     2400

agauguauuu auuccggcaa gaaacuuaac uugucaaggc ugcgcaagag uaauuaccag     2460

aacgauaucg acuauauaua ccccuuaucu gccguuaaug accggagcau gaacaauaaa     2520

guccucacug ggauccagga aaacagagcg gauaaguaua ccuacuuccc gguggacccu     2580

gagauucaga agaaaaugaa gggauuuugg auggagcugg ugcuucaagg uuucaugucc     2640

aaagaaaagu auuuuaggcu gucacgcgag aaugauuuca gcgaaaacga guuggucucu     2700

uucaucgaac gagagauuuc cgacaaucag cagaguggcc ggaugaucgc cagcguucuc     2760

caauacuacu uucccgaguc uaaaauagug uucgugaagg aaaagcugau uuccucauuu     2820

aaacgugauu uccaccuaau caguagcuau gggcacaacc aucugcaggc agcuaaggac     2880

gccuacauca caauuguagu cggaaaugug uaucacgcua aauuuaccau ggauccagcc     2940

aucuacuuca agaaccauaa aagaaaggac uaugaucuca acaggcuguu ucuugagaau     3000

auuucgcgcg acggccagau agcaugggaa uccgguccuu acgggucuau ccagacuguu     3060

cggaaggagu augcccaaaa ccacauugcu gugacgaaaa gagucgugga aguuaagggc     3120

ggauuguuca aacagaugcc ccugaagaaa ggccacgggg aguacccauu aaagacaaau     3180

gaucccaggu uuggaaacau cgcgcaguac gguggcuaua ccaaugugac ugggagcuac     3240

uucguacucg ucgagucaau ggaaaagggc aaaaagcgaa uuagucugga guaugugccu     3300

guguaccuuc augaacgccu ggaggacgac ccaggacaca aacuacucaa ggaguaucug     3360

gucgaucauc ggaaauugaa ccacccgaag auccuguuag caaaaguuag aaagaacucc     3420

cuccugaaga uagacgguuu uuacuauagg cuuaaugggc guucuggcaa ugcccugauc     3480

uugaccaacg cuguggaacu cauuauggau gacuggcaga caaaaaccgc caauaagauc     3540

agcggauaca ugaaacgccg ggcaauugau aagaaagcca gaguauacca aaacgaguuc     3600

cauauccagg aacuggagca gcuauaugac uucuaccugg auaaacuuga aaauggcgug     3660

uauaagaaca ggaaaaauaa ccaggcugag cucauacaca augagaagga acaauuuaug     3720

gagcugaaaa cugaagacca gugcgucuug cugacagaga uuaagaaauu auucgugugu     3780

ucccccaugc aggccgaucu cacgcugauc gggggaucaa agcacaccgg caugaucgca     3840

augagcagua acguuacuaa ggcggacuuu gcugugauug ccgaagaucc ucuuggucug     3900

cgaaacaaag ucauauacuc ucauaugggg gagaaguga                            3939


<210>  2924
<211>  3942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2924
augcacuacc uggacgagau cuucgcuccc caucuccagg aaguggauga gaacuuucuu       60

agaaggcuga agcaaagcaa uauucacccu gaagacccag ccaaaaacca ggcauucaua      120

ggcaaguugu uauuuccgga ucugcuaaag aagaaugagc ggggguaucc cacccucauc      180

aaaaugcgcg acgaacugcc ugucgagcag cgagcccauu acccaguuac aaacauuuau      240

aaacuucgug aggcgaugau caaugaagau agacaguucg accugaggga gguguacuug      300

gcuguacacc auauuaugaa guaucgggga cacuuucuca acaaugccuc cguggauaaa      360

uucaaggucg gucgcaucga cuuuuacaaa ucuuucaacg ugcugaauga agcauaugag      420

gagcugcaaa acggcgaagg gucauuuacu auagagccca guaagguuga aaagaucgga      480

cagcucuuac uggauacgaa gaugagaaaa cuuaauaggc agaaagccgu ggcuaaguug      540

cuggagguca aaguggcaga caaggaagag accaaacgga acaagcagau ugccacagcu      600

augagcaagc uaguacucgg cuacaaggcc gauuucgcga cuguugcaau ggccaauggu      660

aacgagugga agaucgaccu guccucugaa accucggagg augaaauuga gaaauuucgc      720

gaggaacuuu cagacgcuca aaaugacauc cugacagaga uaaccagcuu guucagucag      780

auuaugcuca acgaaaucgu gccuaacggg auguccauuu cugagagcau gauggaucga      840

uacuggacuc acgaaagaca gcuggccgag gucaaggaau auuuagcaac acagccagcu      900

uccgccagga aagaguuuga ccaaguguac aauaaguaua ucggacaggc gcccaaagag      960

cggggcuucg aucuggaaaa gggccucaag aaaauccuuu caaagaaaga gaacuggaag     1020

gaaauugacg agcuguugaa agccggggau uuccuaccua agcagcguac cagugcaaau     1080

ggagugauac cacaucaaau gcaccagcag gagcuggacc gcaucauuga aaagcaggcu     1140

aaauacuauc cguggcucgc cacggagaac cccgcuacug gugaaagaga uaggcaucaa     1200

gccaaguacg agcuggacca gcuugucagc uuucggaucc cuuauuacgu uggcccacug     1260

gugacccccg aaguacagaa agcaacaucu ggggccaagu ucgcuugggc gaaacgaaag     1320

gaggauggag agauaacucc uuggaauuug ugggacaaga uugaucgcgc agaauccgcc     1380

gaggcuuuua ucaaaagaau gaccgugaag gacacauacc uccugaacga agacgucuua     1440

ccagccaauu cacugcuaua ucagaaauac aacgugcuca augagcugaa caauguuagg     1500

gugaacggcc ggcgccuuag cgucgguauc aagcaagaua uuuauaccga auuguucaaa     1560

aagaaaaaga cggugaaggu aagugacguu gcaucucugg ugauggccaa aacuagaggg     1620

gucaauaagc ccuccgugga gggacucagc gauccgaaaa aguuuaacuc gaaucuggcu     1680

acauaccuug accugaaauc uaucgucggc gauaaggugg acgauaacag guaucagacc     1740

gacuuggaga acauuauaga auggcguucc guuuucgagg auggcgaaau cuuugccgac     1800

aagcucacug agguggaaug gcugacagau cagcagcggu cagcauuagu caaaaagcga     1860

uacaaagggu ggggaagacu gaguaagaaa cuacucaccg guauuguaga cgagaauggc     1920

caaaggauca ucgaucugau guggaacacg gaccagaauu ucaugcagau ugugaaccag     1980

ccuguuuuua aggagcaaau agaccagcuu aaucagaagg cgaucaccaa cgaugggaug     2040

acauugcgcg aaugggugga gagcguccug gacggagcuu auacuucucc ccaaaauaaa     2100

aaggccauuu ggcaaguggu acgggugguu gaagauaucg ucaaagcagu gggcaacgcc     2160

ccaaagucca uuagcaucga guucgcuaga aaugagggua acaaagggga aauaaccagg     2220

ucacgccgaa cacagcucca gaagcuguuu gaggaccagg cccacgaauu agugaaggau     2280

accagucuga cugaggaacu ugagaaagca ccugacuugu ccgaucggua cuaccucuau     2340

uucacacagg gcggaaagga cauguacacc ggcgauccca ucaauuuuga cgagauuagc     2400

acuaaauaug auaucgacca cauucugcca cagucuuucg ucaaggauga cucacuagac     2460

aaccguguuc uggugaguag agcugaaaau aacaaaaaga gcgauagggu gcccgccaaa     2520

cucuacgcgg ccaagaugaa gccuuauugg aaucagcugc uuaaacaagg guugaucacg     2580

cagcgcaagu ucgagaaccu gacaauggac guagaucaga acauaaaaua ccgguccuua     2640

ggauuuguca agagacagcu cguggaaacc aggcaaguua uuaaacugac ugcuaauauc     2700

cuugguucua uguaucagga ggcaggcacc gacauuaucg aaacacgagc cgggcugacc     2760

aagcaguugc gcgaggaguu cgaucuccca aaggugcggg aagucaacga cuaccaucac     2820

gcuguggaug ccuaccugac uacauuugca ggacaauauc uaaauagaag guacccgaaa     2880

cugcguucgu ucuuuguuua uggcgaguac augaaguuca aacaugguag cgaccucaag     2940

cugcgcaacu uuaauuucuu ucacgaacuu auggaggggg auaaauccca gggcaaggug     3000

guagaccagc agacgggaga auugauaacc acucgggaug aggucgcgaa gucauucgac     3060

agacuguuaa acaugaaaua uaugcucgug aguaaggagg ugcaugacag gucugaucaa     3120

cuguacggcg ccacaaucgu caccgcuaaa gaaagcggga aacuuacauc ccccauugag     3180

aucaaaaaga agcgaccugu ugaccuguau ggagccuaca cuaaugguac cucagcauuu     3240

augacgauua ucaaauucac cggcaacaag ccaaaauaca aggugauagg gguacccaca     3300

acuagcgcug ugggacuaaa acgggucggc aagccugguu cugaauccua uaaucaggag     3360

cuccaccgca uuaucaagag uaacccaaaa gugaagaaag auuuugagau cguugugccc     3420

cacgucagcu acucucagcu gauuguggac gaugacugca aguucaccuu ggccuccgau     3480

acauaucagc auccggcaac ucaacugguu uuaucaaaag aaaguaugga gaucauugcc     3540

gacgauuuca aguuugugaa agaaaauacc gcuacggcgg acgagcagcu cgucagagua     3600

uucgaugaaa uagugaacca gaugaaucac uacuuuacaa ucuucgacca gaggagcaac     3660

cggcaaaagg uuaccaaggc ccgagauaaa uuugugucgc ugccuacuga guccgaguau     3720

gaaggggcaa agaaaacaca ggucggcaag accgagguga uuacuaaucu ucugauggga     3780

uugcaugcua acucugccca gggcgaccuc aaaguacugg ggcuaagcac cuucggauuu     3840

uuccagucaa guggcggucu gaaccuuucc gaagacgcca ugaucgugua ccaaucucca     3900

acagggcucu uugagagacg cauuugucug aaggauaucu ga                        3942


<210>  2925
<211>  3942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2925
augagcuuca aggagaacuc caaaaucuac uauggccugg acauugggac cgauucugug       60

ggauggucaa caacuaauga aaaguacgag cuccuuagau auaaaaacaa ucugaugugg      120

ggugucaguu uguuugaagc cgcuagcuua gcagaggaaa ggcggggcca ccgcacgcag      180

cgacguagau acgacaggcg gcaacagcgc guugcccugc uaggggaacu cuucgcgaag      240

gagauacuga aaaccgaucc cgacuuuuuc cuuagacuga aggaauccuc uuuguauccu      300

gaggauagga caucgaagaa cgugaauacu uacuuugacg augcugacuu caaagauuca      360

gacuauuuua agauguaccc aaccguacau caccucauca aagaacugag cgagaguaac      420

aagccgcaug augugcggcu ggucuaccuc gccugcgcau ucauuguggc ccaccgcgga      480

cacuuuuuaa auggcgcuga cgagaacaau guucaggcag ugcuggauuu cgacuccucu      540

uauugugaau uuacagacug guucaaaagc aacgauaucg aggacaaucc cuuuuccgaa      600

ucagucaaga acgaguucag ugugauucuu cgaaagaaaa ucgguauaac cgccaaggaa      660

agagagauca aaaauuugcu guucgggacu acaaagaccc cugauugcua caaagacgag      720

gaauauccaa uugauaucga cguacuaauu aaguuuauca gcggaggcaa aacgaaccuc      780

gcuaagcugu ucaggaaucc cucuuacgau gagcuugaca uacagacugu ugaagugggc      840

aaggccgauu uugcggacac cauugaucug uuggcauccu caauggagga cacagacguc      900

ccucuccuga gcgccgugaa agcuauguau gauuggaguu uacugaucga cgugcucaag      960

gggcagaaaa cuauuucuga ugccaagguc ugugaauacg agcagcauaa auccgaccuu     1020

aaggcacuga aguauaucgu ucggaaauac uuggaucgug cucaguacga cgagaucuuc     1080

cgcaccgccg gagaaaagcc aaacuaugug agcuacucgu auaacguaac agaugugaaa     1140

cuaaagcagc ugcccucuaa uuuuaaaaag aagaacuccg aggaguucug caaauacauu     1200

aagucaaaac ucgagaagau aaaaccugaa agugacgaug aggcggucua uaaugagcug     1260

aucgaaaagu guaacagcaa gacccuuugc ccaaaacaag ugacggacga gaauagaguu     1320

auuccguacc agcuguauua ccacgaauug uccaugaucc ucgauaaggc cucugcauac     1380

cuggacuuuu uaaacaaaac ugaggauggu auaagcguga agcagaaaau ucugacacua     1440

augaaguuca ggauccccua uuuugucggc ccucucguga aacggaauga gaccgacaac     1500

guauggaucg uucgaaaggc ugaagggcgc auuuacccau ggaauuucga gaacauggug     1560

gacuuugaua agucagaaga cggauucauc agaaggauga cuuguaaaug cacauaucug     1620

gccggcgagg auguccuucc caaguacagu uugcuguauu ccaaauacac cgugcucaau     1680

gaaauuaaca auauaaaggu caacggugag aaaaucagcc cugagcugaa gcaggacauu     1740

uuuaaugaac uuuucaugaa gacgucucgg gugaccguua aaaagaucac agagcuguug     1800

aaacgcaagg gggcuuucuc agaagagaaa ggagauaguc ucagcggcgu ggacaucaac     1860

auuaaguccu cucugaaguc guauuuagau uuuagaaggc ugcuagaaaa cggcagccuc     1920

uccgagaaug acguagagcg uauaaucgaa cggauuacug ucaccacaga uaaaccacga     1980

cuggugucau ggcuuaaggc cgaguacagu gacuugcccg cagaagauau cagauacauu     2040

ucuaggcuga gcuauaaaga cuacgggcgc cucuccgcca agcuguuaac uggauguuau     2100

gagcuggaua ccaacacagg ugaaaucggc gggcggucaa uaaucgacuu caugugggcu     2160

gagaauauua accuuaugca aaucuugagc gacucuuacg gauauaaauc cuuuauugag     2220

gaagagaaua agaaguacua uaugaucaac ccgaccggca guauagcgca gacucucaga     2280

gaaauguacg uuagcccuuc uguguccagg gcaauuaucc gcacgaugga gauugucaaa     2340

gagcugcgaa agaucaccaa aaaggauccc gacaaaauau ucguggaaau ggcccggggu     2400

gggaagccag aggaaaaagg caagcguaca ucaaguagaa gggagcagau cgaaaagcua     2460

uacgauagcg cuaaagccuu uguaucggac gaggauauuu cccauaugcg cucucaacug     2520

ggaagccucu cagacgagca gcugcggaga gaaaaguauu accuuuauuu cauccaguuu     2580

ggcaaaugca uguacagugg ggaggcaauu gauuucucca gguugggaga caaucacugu     2640

uaugauaucg accauauauu uccucagucu aagauuaacg augacagccu gcacaauaaa     2700

guguuaguua agucccaacu caacggugaa aagucagacg auuaccccau caaagccgag     2760

auccgaaaua agaugcaccc acuguggaaa aaccuuuucu aucgcgaccc caagaauccu     2820

gcugauaaag ucaaguacga acggcugacu agaagcaccc cauuuacaga ggacgaguug     2880

gccggcuuca uugaaaggca gcucguggag acccgucaga guacuaaggc aguggcgaca     2940

cugcuaaaag aaauguuucc ggauucuaag aucgucuacg uuaaagcugg gcaagugucc     3000

gaguuccgcc augacuucga uaugcugaag ugccgggaaa uuaacgaccu ccaccaugcc     3060

aaagaugcau aucugaauau agugaucgga aacguacacg acgucaaguu uacgagcaac     3120

cccaugaauu ucgugaagaa cgccgauaaa cauuacacca uuaagguuaa agagacucuu     3180

aagcacaaag ugucgagaaa uggcgagaca gcuuggaacc cugaaaccga cuuugauaca     3240

gucaagagga ugaugucaaa gaacucugug cgauauguuc gguacugcua uaaacgcaag     3300

gguggguucu uuaaucaaaa uccagacaga gccggcaacc ccgacuuggc aaggcugaaa     3360

aaggauuuag acccugugaa auacggaggc uauaauagua aguccaucag cuguuucucu     3420

cucauuaaau gcacuggggu aggagcuguc aucauaccag uggagcugcu uugugaaaag     3480

cgguacuuua gcgaugacuc cuucgccuca gaguacgcgu auagugugcu gaagaacgcc     3540

cuacccgcua aaaauaucgc aaagauuagc aucgaugaca uuucuuuucc gcucaaacga     3600

agaccuauca agauaaacac ccuguucgag uuugaugguu accgcgucaa uauuaggucc     3660

aaagacucau aucacuacuu cacgaucagu agcgccaugg cugccaucua uuccaaggau     3720

accucugacu acauuaaggc aguuucgagc uauauagaua aaucagacaa gggcuccaaa     3780

uucaagccag gggaagcguu ugauguguug aguaaccuga aaguauacga cgagaucgcc     3840

aagaagugca uuucugaacc cuucagcaaa aucuccaagu uagcugaggc cggaaaacgu     3900

uggaagaaag aagagauuuc acuccagaau ugucugcuuu ga                        3942


<210>  2926
<211>  3945
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2926
augcugaaga aagacuacag caucggccuc gauauuggga ccaacuccgu gggacacgcc       60

gucguuacag acgauuauaa ggugcccacu aaaaagauga aaguauucgg ugacacgucu      120

aagaagacca uaaagaagaa uaugcuuggc gugcuguugu uuaacgaggg gcagacagcu      180

gcagauacua gauuaaaaag gggagcccgg cgccgauaca cccguagaaa gaauaggcug      240

cgguaucuac aagaaaucuu cgcgccugcu cucgccaaag ucgacccaaa cuuuuucuac      300

cgccuggagg aaucaagucu uguggcagag gauaagaagu augacguuua cccgauuuuu      360

ggcaaaagag aagaggagcu guuguaucau gauacacaca agaccaucua ccaucucagg      420

agcgaacugg ccaauaacga ccggcccgcu gaucugcgcc ucguguacuu agcacuggcc      480

cacauuauca aauaucgagg uaauuuccuu uuggaggggg aaauagaccu gagaacuaca      540

gauaucaaca aggucuuugc ugaguucucc gagacccuaa augaaaacuc ugacgagaau      600

cucggaaaac uggacguggc cgauauuuuu aaggacaaca cguucucgaa aacuaagaag      660

ucagaggaac uucugaaauu gagcggcgcg aagaaaaauc agcucgcaca ucagcuguuu      720

aagaugaugg uaggcaacau ggggaguuuc aaaaagguuu uaggaaccga ugaggaacac      780

aagcuguccu uugguaaaga cacauacgag gaugaccuca augaucuucu ggccgaagcu      840

ggcgaccagu auuuggauau cuucguggcc gcaaagaaag ucuacgacgc ugccauucua      900

gcgucuaucc uggaugugaa ggacacucaa accaaaacag uguucagcca ggccaugaua      960

gagagguaug aggaacacca gaaggaccuc auugaacuga agcgggucuu uaaaaaguac     1020

cuuccugaga aaugccauga uuucuuuucc gagccaaaga ucucagggua ugcaggauac     1080

auugacggca aaguuaguga agaggauuuc uacaaguaua ccaagaaaac gcugaagggu     1140

auccccgaaa cugaggaaau cuugcagaaa auugacgcua acaacuaccu ccguaagcaa     1200

cgcacauuug auaauggggc cauaccucac caggugcauc ugaaagaguu aguagcuauc     1260

guggagaacc agggaaagua uuacccauuc cugagagaaa auaaagacaa guuugagaag     1320

auucuaaacu ucaggauccc cuauuacguc ggcccucucg cccggggcaa uagcaaauuu     1380

gcauggcuga cccgagccgg ggaaggaaag auaacuccau auaacuucga ugagaugauu     1440

gacaaagaga caucugcuga agauuuuauc aagcgcauga ccaucaauga ccuuuacuug     1500

ccgacggagc ccgugcugcc uaaacacucc cuccuguacg aaagauauac cauuuucaac     1560

gagcuugcgg guguuaggua cgugacagaa aauggcgagg caaaguauuu ugaugcccag     1620

acuaagcggu caaucuucga gcuguucaaa uuggaccgca aggucagcga gaaaauggug     1680

auuaagcacc ucaaaguagu uaugccagcu auaagaaucc aagcccugaa gggguuagau     1740

aacggaaagu uuaaugcaag uuacggcacc uauaaagacc ugguggacau gggugucgcc     1800

cccgaacuac ucaacgauga agucaauucu gagaaguggg aagacauuau caaaacacug     1860

acuaucuucg aggggaggaa acuuauuaaa cgucgguugg agaacuaccg agauuuucug     1920

ggcgaagaca uacucagaaa gcuguccagg aagaaauaua ccggaugggg ccgcuuaagc     1980

gcuaagcugc uugaugggau cuacgacaaa aagacacaua aaaccauuuu ggauugucuc     2040

augacugagg acuacucgca gaauuucaug cagcugauca acgaugacac guauucuuuu     2100

aaggaaacaa uuaaaaacgc ccaagugauc gagaaggaag agacccuagc aaagacugug     2160

caggagcugc cuggaucccc agcgaucaaa aaggguauac uccagucacu ggaaauuguu     2220

gaugagauca uuaaagugau gggcuacaag cccaaaagua ucguagucga aauggcucgg     2280

gagacccaga agacacacgg gaccagaaag agggaggacc gcgugcaaca gauaguuaaa     2340

aaucuuaagg augccaacga auugccgaaa aagcugccua gcaaugcaga guuauccgac     2400

gaacgaaaau aucucuacug ccugcagaac ggacgggaca uguauacugg cgcccccuug     2460

gauuacgacc aucugcaguu cuaugaugug gaccacauua ucccacaauc uuuucuuaag     2520

gaugacagca uugagaauaa gguccucaca aucaaaaagg aaaacgugcg uaaaacgaau     2580

ggucugccuu cagaggcugu aauacagaag auggggaguu ucuggaaaaa gcuacuggau     2640

gccggcgcaa ugaccaacaa gaaauacgac aaucucagaa ggaaccugca uggaggccuu     2700

aaugagaagu ugaaagaacg cuuuaucgag cggcagcugg uggaaacuag acagauuaca     2760

aaguacguug cucaauuacu cgaucagagg cugaacuaug acgggaaugg agucgagcuu     2820

gaugagaaaa ucgccauugu gacccugaag gcgcagcuag ccucccaguu ccgaagcgaa     2880

uuuaagcucc gcaaagugcg ggcucugaac aauuugcacc acgcacauga cgccuaccug     2940

aacgcugucg uugccaacuu aaucauggca aaguaucccg agcucgaacc agaguucgug     3000

uacgguaaau auagaaagac aaaauuuaag ggccugggga aagcgacugc caagaauacc     3060

cuuuacgcua acgugcugua uuucuugaag gaaaaugagg uauaccccuu cugggacaaa     3120

gccagggauc ucccuacgau aaagcguuac cuguaucgcg cacaagucaa caaagugcgg     3180

aaggcugaaa gacagaccgg aggcuuuucu gacgagaugc uaguuccaaa aucagauagu     3240

gguaagcugc uuccgaggaa ggaggggcuc gaccccguga aauacggcgg auaugccaag     3300

gcagucgaaa gcuacgccgu gcugauuaca gcugaugagg uuaaaaaggg caaaacuaag     3360

aaggugaaaa ccuugguaaa uaucccuauc auugacucca agaaauauga agcggaucca     3420

acagccuacc uggcaucucg aggguauacu aacgucacca auucguucau cuuacccaag     3480

uacagccucc uggaggaccc ugagggacgg cgcagauacc uugcuuccuu uaaagaguuc     3540

cagaaggcca acgaacugau uuugccacaa caccucgugg aacugcuaua uugggugaau     3600

gccaaggaug gcgagcagaa acuggaagac cauaaggcug aguuuaaaga gcuuuucgau     3660

aagauaaugg aauuugcaga caaauacguc guugccccca agaacucaga gaaaaucagg     3720

cggcucuaug aagagaauca ggaugcgacg ccgauggaac uggguaagaa cuucguggag     3780

uuguuacgau acacagcuga cggggccgca agugacuuua aguucuuugg agagaauauu     3840

ccuagaaaac gcuauaacuc ugccggcagc cugcucaaug guacccugau cuaccagucc     3900

aagacuggcc uuuaugaaac aaggauugau cuggggaaau uguga                     3945


<210>  2927
<211>  3948
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2927
augggcuaca gcguggggcu ggacaucgga accgguuccg ucggcugggu ugugacaaac       60

gaggauggga agcucgccag agcuaaagga aagaaucuua uuggcguaag gcuguucaaa      120

acugcagaac ccgccgcgga ccggcgcacg aaccgaacca cacguagaag guugucucgg      180

cgcaagugga gauuaaggcu gcuagaugag cucuuugcua augaacuggg uaaaauagac      240

ucaaacuucc uuacucggcu gaaguauagu uacgugcacc cuaaggauga cgccaaucgc      300

gcaaacuauu acgggggaua uuuguuucca acccaggagg aaacuaaaaa guuccaugcc      360

aaauacccga ccaucuauca ccuccgacug gcucugauga cugagagcag acaauuugau      420

cucagggaga uuuacuuagc aaugcaucag aucgucaagu accggggcca cuuccuaaau      480

ucccagucua aaaugacaau uggcaacacc uauaaucccc aggaccuuca aacggccaug      540

gaaaacuacg cugaggccaa gggguugucg aucgaacuga agcagccuga ggagauggug      600

gcgauacuaa cuggagaagc agguuuuggc cucagcaaaa aggccaaagc ugagaagcug      660

cuuaauaacu ucuuuuucga uaccaaagaa gagaaggcca caguucaggc aauccuggcu      720

gggauugugg gaaauacuau cgacuuuacc aaagucuuca accgucaguu gacaggcgau      780

acccucaagg ccuggaagcu gaaauuagac agcgaaacgu uugaugugga cagugagcca      840

auucuguccg accucgauga cgaugagacu gaacuuuucu ucgcgauccg ccaagccuuu      900

gacgguuuca cacugaugaa uuugcuaggg gaucagaccu cuauaagcgc agcuauggua      960

gagagauaua cucagcauca cgacgaucug cucacaguua aacagguggc caaaaagcaa     1020

ggacuguccc acaaagaauu uucaaacauu uacagugcuu uccuuaagga cgauaccgac     1080

aagggcauga aaaagcuguu ggaggaaagc aaccucucua cggaggaugu caauggcauc     1140

cagcugagga uggacuccca ugacuuuuua ccccagcaac ggaccaaagc caacucagug     1200

auuccucacc agcugcaucu agcagagcuc gaagccauca ucgagaauca ggggcaguau     1260

uacccauucc ugcuugaaac auuugagaag gaaggaacug cugcgaacaa aauugcagag     1320

uugcugaagu uccgaauacc cuauuacgug gguccuaugg ucaccaagaa agauguugag     1380

aaugccggcg gggacgcuac aaaccacugg gugacucgca augaaggaua cgaaaagagc     1440

gccguaaccg cauggaacuu ugaucaagug uucaauagag accaggccgc ucucgauuuu     1500

auccagaggc ugacaggcac cgacacuuau cuuauuggug aaccaacgcu gccgcagaac     1560

aguuugaaau accaaauguu cacaguccuc aaugagcuga acaaugugaa gguuaacggg     1620

cggcgcaucg auaaucagac caaacacgac uuaauacagg aucuguuuaa gucuaagaaa     1680

acuguguccc uaaaggccuu ccaggacuau uaccaaagcc agggcaaauc ggauauucag     1740

gucgugggac ucgcagacaa gaccaaauuc aacucuaacc uguccacaua uaucgaucuu     1800

ucaaagaccu uuagugcgga ggaaauggag aauccccaga accaagacuu gcuggaacag     1860

aucguagaga uucagacugu uuucgaagac agcaaaaucg cugagagaga gcucuccaag     1920

cuggaauuaa caccugagca agugacgaag cuggccaaaa cccauuacac uggcuggggg     1980

aaucuuucua acaaguugcu cacaacccca auugucgaug ugagcggaaa gaaggucuca     2040

auacuggacc agcuagcaaa aacaaguaag aauuuuaugu ccaucauuag caacgaacag     2100

uauggugugc aggauuggau caaggagcaa aauacuaccg aaacgaccgg cacacuggag     2160

gacaaacucg ccgaucugac uaccucuccc gcuaacaaga gggggaucaa acaggccuuc     2220

aauguucuuu uugacauuca gaaggcaaug aaaucagagc cugaucgugu guacuuggag     2280

uucgcucggg agacacagac aaguguacga accaugagca gauacaacua ucugaaggga     2340

uuauaccaau ccaagucucu cgacgccgaa gcgaaaaauc ugaagaaaga gcuugccgaa     2400

acggaucaga agaaauuuga caacgauagg cuguauuugu acuuccagca gcaaggcaag     2460

gacauguaua caggucagcc aauaaauauc gauaagcucu cgagcgacua cgacauugau     2520

cacaucauuc cccaggcuuu uaccaaagac gauuccaucg acaaccgcgu ccugguguca     2580

cggccggaga augcaagaaa gagugauucu gggacuuaua caaccgaggu ucaagccgcu     2640

gccagcggcc uauggaaaac ucuggugcag uccggauuca uaucaaggaa gaaauacgac     2700

cgccucaccc augcaggcga uuacagcgaa gggcagaaga caggauuuau cgcgcgacag     2760

cuggucgaga cgcggcaaau uaucaaaaac guggccgcuc uuauugaauc ugaguucgac     2820

cagacccagg ccguagcaau ccgugcugag auaacuggug auaugagaag guugguggcc     2880

auuaagaagc accgcgaaau caacuccuuu caucacgcau ucgacgcccu guuaauuaca     2940

gcugcgggcc aguauaugca aaaacgguac ccugauagag acaguaauaa cguuuauaau     3000

gaguucgaca gguacaccaa cgauuaucuc aagaaacugc gagaaucauc uuccaaggac     3060

gagguccgca aacuuaagcc cuuuggguuc aucgugggaa caauggccaa gggcaaugaa     3120

gauuggacua ccgagcagac ggaguaccug cggcacauaa ugaacuuuaa aaauauccua     3180

acaaccuaua agacuggucc agacagaggg ccucucucaa aagaaaccau uuacgcagug     3240

gaucccaagg cuaaacugau cggcacaaac aagcauaggc aggacgucag uuuguacgga     3300

ggcuauacuu acccauauag cgccuacaug acccugguuc gugccaaugg gaagaaccag     3360

uuagugaaag ugucgcgcuc cgcugcaucu cucaugaaaa gcggacaacu gaaacuugag     3420

gauuauguac agcagcagcc cgaagucaag aaauucgaga agauucuguu ggaacaccuc     3480

ccucugggcc aacuggugga caaugauggu aagcugaugu accuuacauc auaugaguuu     3540

uaccauaacg ccaaacagcu cuggcugagu acggagaaug cggacuugau cuccucucug     3600

agcauagauu ccgacgauca gaaguuaauu aaggcuuucg acauccucac cgccgacuca     3660

guuaucgaac gguuuccauu cuacgagauu gaucugaaga aacuuaucga acugagagac     3720

aaguuugugu ugauugauaa caagcucgac guccugaggg ugguucuaaa agcacugcaa     3780

cuugauagcg cccagcagaa gccgcucaaa cugauagaca aggcugcaac ugauuggaaa     3840

aauuaucgac aggccggggg aaucaaguug agugacaacg cggagauuau cuaccaaucu     3900

acaaccggca uuuucgaaaa acggaucaag auauccgauu uacuguga                  3948


<210>  2928
<211>  3951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2928
augcugcaga agaacuacac caucggccuc gacauuggga caaauagcgu gggauaugcc       60

gucauaaacg auaauuacaa aguucccgcu aagaaaauga aggugcuugg uaacacugac      120

aaaaagacga ucaagaaaaa ucugaugggc guauuguuau ucgaugcagg ggagaccgcc      180

gcgacaacua gacugaagag gggaacccgg cgccgauaua cacguagaag gaaccggcua      240

cgcuaccucc aagaaauucu gggcccugag cuugcuaaag uggacgccaa uuuucugcac      300

agauuggcag aauccucucu cgucaccgag gauaaggaau uuucagccua uccaaucuuu      360

ggugacaggg aggaggaacu gcuguaccau aaaacuuaua agacaauuua ccaccuccgg      420

agugcuuuag caaacaagga ugagaaagcc gaccugcgcc uuaucuacuu ggcucuggcc      480

cauauaauca aguaucgagg gaauuuccua auugaaggag agaucgagcu cagcgauacc      540

gacauugaua aaguguacgc ggacuuuugc agauccuuca acgaacuguu ugauacggag      600

guucuucagg aaaucgaggu ggaagcaaua uucaaggaca aaacucuguc uaagaccaaa      660

aaggccgagg aguuggucaa ggugucgggc guaaagaaga aucagguugc ucaccaguuu      720

cucaaaauga uggugggcaa cacagucaau uucaagaaag uguuuaaccu gacugaagac      780

caaaaguuau cauucgggac cgauagcuau gaggacgaac ugaaugaucu ccuugccaag      840

agugacgaua gguaccugga cguguuuguc gcagcuaaaa agguuuauga uacagccauu      900

uuggcgaaca uccuaaacac caaaaauucc guggccacga agacuauuuu cucucagagc      960

augaucgagc gguucaaaga ccaucagaag gaucugaagg aacucaaaca gcuguuuaag     1020

acauaccuuc cggagaaaua ucccgacuuc uuuggaaacu ccaaggagga ugguuacgca     1080

ggcuacaucg aagggaaaac caaugaggaa aaguucuaua cucacacaaa gaaaauucug     1140

aagaacauac cucaagcugc cucaguauug gcucagaucg acgugggaaa uuaccuccgu     1200

aaacagcgca ccuuugacaa cggcgucauu ccacaccaga uccaacugaa ggaguuacug     1260

gccauaauug aaaaucaggg uaaauauuac cccuuccuac ucgagaacaa ggagaaaauc     1320

gcaaagaucc ugacguuuag gauuccuuau uacguggggc cacuugccag aggaaauagu     1380

cgguucgcuu gguugaagcg aguuggcaaa gaaaagauca cccccuuuaa cuucgagaau     1440

guggucgauc uggaaagcuc ugcggagcgc uuuauugaga gaaugacacu caacgaccug     1500

uaucuuccug aagagaaagu gcugccaaag cauuccaugu uguacgaaaa auucucaaua     1560

uuuaaugagc ucgcaggcau cagguacauu acugauaacg gggaaaugaa guuccuggac     1620

gcccagacca agcaaagcau cuucgaguua uuuaaacagg agcggacagu aaaggaaaag     1680

aagguucugg aucuacucaa aacucugaug ccgagugagc gcguggucgc uaucaaggga     1740

auugacaaug guaaguucaa cgccucuuuu ggcaccuauc acgaucuuuc caaaaugggg     1800

uucaagacag agaaaauagc aagcgaaucg aaccaggcca gaugggaaga gaucauuaag     1860

accuugacua ucuuugagga caggaaacug auuaagucuc gucuccggaa guacgaugac     1920

uuccugaaug aaaaagagau cggagcuuua uccaagcgaa aauauacggg cugggguaga     1980

cugucagcca aacuuauaga ugggaucuac gacaaaaaga cacaucagac cauuuuggau     2040

uuucucauga acgaagacua uaaucaaaac uucaugcagc ugaucaguaa ugacaacuac     2100

agcuuuaaaa agauuaucga ggauucccag gugauaaagg aagagggcuc ucuaacugag     2160

gucauucagg aacugcccgg aagcccugca aucaaaaagg gcauucucca aucaaucgag     2220

auaguggacg aacugguuaa agugauggga gggaauccaa agaaaaucgu agucgagaug     2280

gcgagggaga cccagaagac acacgguacc acucgccggg aagcuagaau uaaggagauc     2340

auuaaaaacc uuaauaaguu gaaacugaac gaauuaccca aggaucuccc uaguaaugag     2400

gaacuguccg acgagaaagu guaucuuuac ugucugcaga acggcaggga uauguacaca     2460

ggggccccau uggaccucga uaaucugagc caguaugagg uugaccacau cauaccccaa     2520

ucuuuccuaa aggaugacuc aauugaaaac aaggugcuga cggcaaaaau cgagaauagu     2580

cgcaagacca acggacuccc gagcgucgaa aucgccaaaa agaugggcgc uuuuuggcga     2640

ucccugcuua aagugggugc cauuucugag aagaaguacc ggaacuugcg uagaucgcug     2700

caugggggca ugacugaaaa auuaaaggag acauucguaa aaaggcagcu cguggagacc     2760

cgccagauca caaaguaugu ugcacagauu cugaauagcc gguuuaacga agagaaaaau     2820

gaagauaagg ucgcuauagu gacucuuaag gcccaacuga ccucccaguu cagaaaagac     2880

uucgagcuau acaaggugag ggaaaucaac aaucuccacc augcgcacga ugccuaucug     2940

aacgcugucu uggcaacgcu guuaaugacc ucauuuccug agcucgagcc cgaguucguu     3000

uacggaaaau aucgaaagac aaaauuuaag ggccugggga aagccacugc uaagaauaac     3060

cuuuacacca auauucugca guugcucaag gaagaaagug cccaauacug ggacacaaaa     3120

gagcgcgacc uggcaacuau caagaaaaac guguaucagg cccaggugaa uaagguacgg     3180

aaagucgaga gacagaccgg aucuuucuac gaugaaacgg uggcgaaaaa gagcgcuucc     3240

caaaaacuau uuccaaggaa gaacggucug gacccuguua aauauggcgg guaccagcgu     3300

gccaaugagg gauauucacu ucucuuccgc gcagauaugc ugaacaagcg gggcaaaaca     3360

aagagcguga agaccuuggu caaaauuccc aucauagaau cugcucagua ccuggccgac     3420

ccaacugagu auuuagcaaa gagagguuac uacaaugugu ccgaacauag ucuccugcuu     3480

cccaaauuua gccuguugga gaacaacaau gggcucaggc gauaucuggc cucuuccaac     3540

gaguuccaga aggcuaauga acuaauccug ccuaaacacc uuguucggcu ccuguacugg     3600

ucacaaagua agcagcgcaa ggaggaauug aacaaacaca gagcggaguu ugccgaauua     3660

uucacauaua uuauggauuu ugcagacaag uacgugguag cuccgaaaaa uagcgagaag     3720

aucaaaaaca uuuaugagaa uaacaaggau ucguccgucg aagugcuggg caaaaauuuc     3780

aucgagcucc ugaaguacac cgccgacgga gccgcuucug auuucacuuu cuucgacacc     3840

aagauaccaa ggaaacggua uaacagcgca ggcgaucuuc uggacgggac auugauuuac     3900

cagucaaaga cgggacuaua cgaaacccga aucgaccugg gcgccaucug a              3951


<210>  2929
<211>  3957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2929
augaugauca agcugaacaa uaugaaaaag auucucggcc uugaccuggg gaccaacagc       60

auaggauggg ccguggucga gacagaagau aauaaaaagu ucgaguuguu agacaaaggu      120

guuagaaucu uucaggaagg cgugaagauu gagaagggga ucgaauccuc uaaagcugca      180

gagaggacug gauaccgguc agcccgccga auuaaguucc guagaaggcu gcggaaaauc      240

gagacgcuaa agguacucau agaaaacaau augugccccc ugaguaugga ugagcuuauc      300

guguggaaag aaaacaagaa uaacuauccu aagaauaaaa ucuuuuacaa cuggcugaag      360

accaaagacc caaaggaggg cacaaauggu acuuauauua acccguacua uuuccgcacc      420

gaggcgauua aaagaaaguu ggaaaauaca aacgaugucg ggagggcucu cuaccaccug      480

gcccaacggc gcggauuuaa aagcaaucga cuggagucca ccgacgaaaa gucuagaggc      540

aucgugaaga cugagauaaa caaacucucg ucagaauuaa acggcaggua ucugggggag      600

uacuucuacg agcuuuauuu gaagggagaa aagauucgga agcgucauau cagccgcgag      660

gaacacuacc ugaaugaguu uaaaaagauu ugugaaguuc agaacuucaa uaaggagcua      720

gcagagaaac ucgaaagagc caucuuuuau cagaggcccc ugaagaguca gaaaggucuu      780

gugggcaagu gcacacugca aaacaauaaa caucgguguc cuaucucccg accagaguuc      840

gaagaguuuc gcauguggca guucauuaac ucuauaaagu acaagaauag cgaugggaaa      900

uuugacuucu ugaacaagga acagagagag aauaucauuc ccgcuuucgu cuccaacaaa      960

aaugcaaccu uugaguucuc aaagaucgag aaaauacuca acaagggagu gcacauuuau     1020

caguuuaauu acaagaguaa ccauacgauc ggcagcaauc aaacuaugau caacuucaug     1080

gauguauuug acauugauag gaaacugacc aacgccuggu uacugugcgu uaauaagauc     1140

uaugaacucu acacagcuaa aacuaagaaa gacgguucuc uuaaguccaa aucagaagug     1200

auuaccgaug ucuggcacgu gcuguucaca uuugacaacg aggagaauuu gaaggaguuc     1260

gcccggaaca agcuacugcu cgacgaaagc agugcgcaga aauuuucuaa gauaaaucug     1320

aaacaggauu acucccagcu uagccugaag gcaaucaaca aaauuuugcc uuaucuccaa     1380

gaagggcuga ucuacucgua ugccguguuc aucgcuaaua uugagaacgu cauaggaaag     1440

ucuaucugga auauuaagga aaaccagcag aucauucgca ccuccaucaa aaagauaauc     1500

gacaaucaca aaacggaguu agauauuaac acuaucacaa auucacugau uaacgacuuu     1560

aagguuaauu acaacaacgc ccauaaagag uauaaggugg augacaucga ucuaaagaau     1620

auaagagaga aaauuaucaa guacuauggc aacaaaaccu ggaauugucu cccagaagac     1680

gaaaagcugu acguaaaaga gaagguggaa aaacuuuacg agaaacaauu gaagaguggc     1740

aaaaggggga uuuucaucga gaagauacgu cuggaugacc ggaucgaaaa cuuucucaaa     1800

gauaauuucg acagcuccga gcugaaccuu aagaaacugu aucaccccuc ugacgucagc     1860

guguacaagu caguuaagau uacugauuug ggacuccgac uguuagguag uccuaugauc     1920

ccauccauua aaaauccgau ggcaaugaga gcuaugcauc agcugaggaa gcuaaucaac     1980

caccucauaa uugaaggcgu gaucgacgau agcacaauca uuaaugucga gaugucucgc     2040

gaacugaacg acgccaauau gcggaaagcg aucgagagau aucagaggca gcgcgccacc     2100

gagaacgaag aguucaagac gaaaauuaag gaacuuuaca agaaagagug cgggaccgau     2160

uuugagccca auaacgacga uauaaugaag uaucgauugu gggaggaaca aaaucacaaa     2220

ugucuguaca caggaaaguc aaucggcauu ugcgacuuca ucgguaguaa cccugcauuu     2280

gaaauugagc auacuauccc acggagccuc uccuaugaua acucucugga aaauaaaacc     2340

uuaugugaga aggaguucaa ccaacaggug aagaaaaauc guauacccuu ugaacugucg     2400

aacuacaagg agaucauuag cagaaucaau aacugggaaa uuaaagcuga ggaacuuuuc     2460

aagcagaucc aaaaaguaaa gauacagucc aggauggccg cuacaaagga gauuaaagac     2520

aaugccaucc agcgcaagca cuugcuccag auggaguuua acuacuggaa ugggaaacug     2580

ucacgguuca cuagagaaga uguuccugag ggcuuuaaga acagucagcu aguggacauc     2640

ggaauuauaa ccaaauaugc aagggccuac cugaagacag ucuucaauaa cgugaauacc     2700

gucaagggcu cuacuguggc ugaauuucga aacaucuggg ggcuccagga guauuucgag     2760

aaaaaggauc gcagcaauca uauucaccau ugcaucgacg cgauuacgau cgcaugucug     2820

acaaaagaca aguacgauuu ucuugcccac uuguaucacc uggacgaaca gaacaaucgg     2880

gaugcuguuc auagagccau gauaaaaucc aagccauggg agaacuucgu gcaggacgua     2940

aaagaaaucg agaaccaaau uuuagucuca cacuacaccc ccgauaaucu cccgaagcag     3000

agcaagaaaa uccugaggaa gcguggaaaa auucagaaga acgaaaaugg ugaccuuauc     3060

uaugagaaag gcgaugcagu gcgcgggucu cugcauaagg aguccuucua cggagccaua     3120

aagcgggaaa uuaucaacaa aaagggcgag acugaagaga aaguuuugua cgugaagaga     3180

aaaccucucg aauuuaguga ccuggguuuc aagaccauca gcgagcuaga gaauauuguc     3240

gaugacgcug ugagggauaa gguacugaac gccgugaaag acaaggggcu caaacaagca     3300

cugaaugaac ccaucuggau gaacgaggaa aagcagauac caauuaaaaa gguucgaugc     3360

uauacaccuu cugucaccaa ucccaucaac cuuaagaaac accgggauaa guccaaacac     3420

gaguacaagc agucauauca ugugacuaau gacggcaacu acuugauggu gauuuaugaa     3480

ggaaaagaca gucaggagaa ggucaaacgc gauuacgaga ucguuagcaa ucugcaagcg     3540

ggcgaauauu uuaagcaguc ggugcacggg guguuaaagg cucagggaau aaacaaucuc     3600

gagggucuga uuccaaaauu caaggaaucc ggcaaaaaca uccuuccccu gaaguacauc     3660

cuaaaaacag ggacgauggu aaucuuuugg gagaacuacc cugaagagau uugggacauu     3720

gauaagaauc agcucuauaa gagacuguuc aaagucaccc aauuugaguc ugacggaagg     3780

auacaggccc gguuccauca gaucgcaaag ccagauaacg aauugacuaa agugagcgag     3840

cuggacuuuu caaauauugc cgaaaagauc cgauuaagua aaggcaaccu caauaugcug     3900

guuaacgguu acgauuucaa gaucaaugug cuugggcaca uugagaagcu guccuga        3957


<210>  2930
<211>  3957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2930
augggcagca acaugcugaa gaaagacuac uccaucgggc ucgauauugg aaccaauucu       60

gugggucacg ccgucguuac agacgauuau aaggugccca cuaaaaagau gaaaguauuc      120

ggcgacacgu caaagaagac cauaaagaag aacaugcuug gggugcuguu guuuaaugag      180

ggacagacag cugcagauac uagauuaaaa aggggcaccc ggcgccgaua cacacguaga      240

aagaacaggc ugcgguaucu acaagaaauc uucgccccug agcucgcgaa agucgaccca      300

aauuuucugu accgccuuga agagaguagc cugguggcug aagauaagaa guaugacguu      360

uacccgauuu ucgguaaaag agaggaggaa uugcucuauc augauaccua caagacuauc      420

uaccaccuga gguccgcccu ggcaaacaau gaccagcccg ccgaucuccg guuaguguau      480

cuggcucuug cacauauuau caaauaccgc gggaacuuuu ugcuggaggg agaaauagac      540

cuacgaacaa ccgauaucaa uaaggucuuc gccgaguuuu cugagacgcu caacgaaaau      600

ucggacgaga accugggcaa acuugacgug gcugauauuu ucaagaauaa cacuuuuuca      660

aaaaccaaaa agagcgagga acuguugaaa cucaguggcg ccaagaaaaa ucagcuggcg      720

caccaguuau ucaagaugau gguagggaac augggauccu uuaaaaaggu ucuggguaca      780

gacgaggaac auaagcucuc uuucggcaaa gauacuuaug aggacgaucu uaacgaccug      840

uuggcagaag ccggggauca auaccuagac aucuuugugg cugccaagaa agucuaugau      900

gcagcuauuc uggccagcau ccucgacgug aaggauaccc agacaaaaac cguguucucc      960

caggcgauga uagagagaua cgaggaacac cagaaggacc ugauugaacu uaagaggguc     1020

uucaaaaagu aucugccuga gaaaugccac gacuuuuucu cagagccaaa gaucagugga     1080

uacgccggcu acauugaugg uaaaguuagc gaagaggacu uuuauaagua cacgaagaaa     1140

acuuugaagg ggauccccga aacagaggaa auccuccaaa agauugaugc aaauaacuau     1200

cugcggaagc agcguaccuu cgacaaugga gcuauaccuc aucaggugca cuuaaaagag     1260

cugguagcca ucguggagaa ccagggcaag uacuauccau uucuacgcga aaauaaagau     1320

aaguucgaga agauucucaa cuuuagaauc cccuacuaug ucggcccucu ggcuagggga     1380

aauucuaaau ucgccuggcu uacucgggca ggggaaggua agauaacacc auacaacuuu     1440

gacgagauga uugauaaaga gaccuccgcc gaagacuuca ucaagcauau gacgaucaau     1500

gauuuguacc ugccgaccga gcccgugcuc ccuaaacacu cacugcuuua ugaacgauac     1560

acaauuuuua acgagcuggc uggcguucgc uaugugacug aaaaugggga ggcgaaguac     1620

uucgacgcac aaaccaagag aagcaucuuu gaguuguuca aacucgauag gaaggucagu     1680

gaaaagaugg ugauuaagca ccugaaagua guuaugccag ccauacggau ccaggcuuua     1740

aagggacugg acaacggcaa guucaaugcc ucuuauggua cauacaaaga ucuaguggac     1800

augggggucg cacccgagcu ccugaacgac gaagucaauu ccgagaagug ggaagauauu     1860

aucaaaacuc uuaccaucuu ugagggccgc aaguugauua aaagaaggcu ggagaacuau     1920

cgugacuucc ucggagaaga uauacugcgg aaguuaagcc gaaagaaaua cacaggcugg     1980

gggagacugu cggccaaacu uuuggacgga aucuacgaua aaaagaccca uaaaacuauu     2040

cucgacuguc ugaugacgga ggauuauucu cagaacuuua ugcaacuaau caaugacgau     2100

aacuacuccu ucaaggaaac aauuaaaaau gcucagguga ucgagaagga agagacccug     2160

gccaagacug ugcaggagcu cccugguuca ccagcaauaa agaagggcau ccugcagagu     2220

cuugaaauug uugacgagau cauuaaagug augggguaua agcccaaaag caucguaguc     2280

gaaauggcga gggagaccca aaagacacac ggaacccgca agcgggagga uagagugcag     2340

cagauaguug aaaacuugaa agacgcuaau gagcugccgg aaaaguuacc uuccaacgcc     2400

gagcucucug augaaaggaa auaccuguau ugccuucaga auggccgcga cauguacacu     2460

ggugcacccc uggacuauga ucauuugcaa uuuuacgacg uggaucacau uaucccacag     2520

agcuuccuca aggacgauuc aauugagaac aaaguccuga caaucaagaa ggagaaugug     2580

cgaaaaacga acgggcuacc uagugaagcc guaauacaga agaugggcuc cuuuuggaaa     2640

aagcugcucg acgcuggagc caugaccaau aaaaaguacg auaaccugcg gcguaaucuu     2700

cauggcgggu ugaacgagaa gcugaaagaa agauucaucg agaggcaguu aguggaaacu     2760

cgccaaauua caaaguaugu ugcacagcuc cuggaccagc ggcuuaauua cgauggaaac     2820

ggugucgagc uggacgagaa aaucgcuauu gugacccuaa aggcccagcu cgcgagccaa     2880

uuuagaucug aguucaaacu gaggaaggug cgagccuuga acaaucugca ccacgcucau     2940

gaugcauauu uaaacgccau cgucgcuaau cucauaaugg ccaaguaccc cgaacuggaa     3000

ccagaguuug uuuauggcaa auaccgcaag gcaaaauuca aggggcuugg aaaagcgaca     3060

gccaagaaca cucuguaugc uaauguguug uacuuucuca aagaaaacga gguguacccc     3120

uucugggaca aggcccggga ccugccuacc auuaagagau aucuauacag ggcacaggua     3180

aauaaagucc guaaggcuga gcgccagauc ggcgguuucu cagaugaaau gcuggugcca     3240

aaaagugaca gcgggaaacu ucucccgcgg aaagagggcc uggaucccgu uaaguaugga     3300

ggcuacgcca aggcagugga auccuaugcc gucuugauca cggcugacga ggugaaaaag     3360

gggaaaacca agaaaguuaa gacacuggug aacauuccua ucauugauuc uaagaaauac     3420

gaggcggacc caacugccua uuuagcaucg agaggauaca ccaauguaac aaacagcuuu     3480

auacucccca aguacucccu gcuugaagau ccugagggca ggcgacggua ucuggcuuca     3540

uucaaagaau uucaaaaggc caaugaguug auccucccac agcaccuggu cgaacuacug     3600

uacuggguga acgccaaaga cggugagcag aaacuugagg aucauaaggc ugaguucaaa     3660

gaacucuuug acaagauuau ggaauuugca gauaaauaug uggucgcccc caagaauagu     3720

gagaaaaucc gcagacugua cgaagagaac caggacgcga cuccgaugga guuggggaag     3780

aauuucguug aauuacugag guauaccgcu gauggagccg caucugacuu caaauuuuuc     3840

ggcgagaaca uuccucggaa gcgauacaac agcgccgguu cccuccugaa uggcacgcuu     3900

aucuaucaau caaagacagg gcuguacgaa accagaauag acuugggaaa acuauga        3957


<210>  2931
<211>  3960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2931
auguucagcc agagaaacaa ucccuacugc gagcugcucu uuaagaucau ugugaccaac       60

auagaagaga aaaaucuuga agucaucaug ggcauuagga acuccgacau cuucuuuauu      120

ucuaauuuca ugugguauca aaagcugauc augaugauaa agaacaucuu gggguuagau      180

cugggaacaa auucaauugg uugggcccua aucgagguug acgaaaacaa uauuccuauc      240

cggauaauug cuaugggcag ucgcaucauu ccacucagca cuuccgauaa ggaggaguuu      300

cagcgagggc ugucuaucac gaaaaaccag aaucguaccg ugucgagaac acagaggaag      360

ggauacgacc ggaagcaacu ucgcagauca gaucugaaaa agaucuugaa aaaguauaac      420

auuuucccga gcgaagaacu ccugaaacug cccaugcucg acuuauggaa gcugaggagu      480

gauuccgcaa auccuaacga agacauaucu gccgagcagc uuggccggau cuuguacaug      540

cugaaucaga agcgcgguua uaaaagcgcg cgauccgagg cuaacucaga uaagaaagac      600

acugauuacg uacaggccgu gaaggggaga uaugcacaac uaaaagacag gcggcagacc      660

auuggacagu acuuuuacga ggagcucagu acagccuuca gcgauaagaa caaucagcaa      720

uauuuucgug ucaaagaaga gguguaccca cgcgaggcuu auaucgaaga guucgacacc      780

auaauuaacg cacagaagga caagcacggc uuucugacug auaaaguuau ccaucagcuu      840

agaaaugaaa ucauuuacuu ccaaaggaag cugaaaucuc agaagggcuu gguguccauc      900

ugugagcucg aaggguuuga gaaaacaguc uaugacaagg aaaagaacaa aaauaaggug      960

guauucguug gacccaaagu ggccccuaag accucaccac uguuccaguu augccggauu     1020

ugggaggucg ugaacacgau aagccugaaa aucaagaauc ccgaggguag uaaguacaaa     1080

uggggcgaaa aggugccuaa ccucaaacag aaggagauuc uugcugauua ucuguuuaaa     1140

aaugaaaacu ugucuuucuc cacucuacug gagauccucg aacugaagaa ggaggacguc     1200

uacguuaaua aacaaauccu uaaggggauu aaaggaaacg aggccuacgc ggcacugcac     1260

aauguguugg gcgaucauga acuccugaac uuugacguaa gcauaaucac cucggaucac     1320

acagccauuu uaguggacaa gaaaacuggu gagauccugg aggaacgagc ugggcuagag     1380

cucgaugccu cucuggaacg cgagccacuu uaucaguucu ggcauaccau uuacuccuug     1440

aaggaccugg augaauguaa aaaugcacuc aucaagagau uuggacugga cgaggacaua     1500

gcugaaaaac uuucacacau cgauuucaac aaacaggccu uuggcaguaa gagcaacaaa     1560

uccaugagga agauucugcc guucuugaug gagggcuaua auuacucuga gagcugcuca     1620

cucgcggggu auaaccacag uaauucccug acaaaagacg aacgggagag ccagaucacc     1680

uuagaacgcc uggagcuacu ccccaagaac aaucugagac aaccuauugu cgaaaagauc     1740

cuuaaccaga ugauaaaugu gguuaacacg auuuuggaga aauacggaaa gccaucugag     1800

aucagggugg aacuggcccg ugagcucaaa cagucaaagg augaacggaa ugacacugau     1860

cugcagaaca gucgaaauaa aaaguuaaac gaggaaauug guaagagacu ggcagagcuu     1920

ggcuugcccg ucacaaaaag guauauccaa aaguacaaau uuauauuccc uagccucucc     1980

aagaaucuga aagacgcuca ugugaacaau caguguaucu acugcgggga gaccuuuaac     2040

cuaacugaag cccugaacgg agaugcuuau gacguagauc acauuguucc aaaggcccuc     2100

cuguucgacg auucucagac aaauaaggug cuuguccauc gcaaauguaa cucgaccaag     2160

acgaaucaga ccgcauacga cuauaucaca gccaaaggcg aggaagaggu gcggauuuac     2220

agcgagagag ucgaugacug guuuaagaaa gguaucauau ccuauucaaa gaugcaaagg     2280

uugaaaguga gucacaagga auaccuggag cgcaagaaau uagggaagga aacugagacc     2340

gacaaaaagc ucugggaaaa cuucauugau cgacagcugc gggacacaca guauaucucu     2400

cguaaaagca gagagauccu ucaaaagguu ugcaauaacg ugacuaccac agagggcgga     2460

guaaccgcua agcugaggaa uuuguggggc ugggaugaca uucucaugaa ccugcagaug     2520

cccaaauaca aggaacuagg gcagacuauc acgaaagagu ggacauccga acacggaaag     2580

cgcaaacauc agaaggagga aauugagaau uggaccaagc gggaugacca cagacaucac     2640

gcgauagaug cacuggucgu ggccuguacu aaacaagguu ucauccagag gauuaacacc     2700

cucaauucaa gcgagacaaa ggaccugaug aucaaagaag uugaggaagc uaagauugag     2760

uuuaacgaga aaaccacucu uuuggaaaag uaccugaaga cacagaaacc guucacgacc     2820

cgagagguga ugaaggaagc cgauaaaauc uuaauaucuu uuaaggcagg caaaaagguc     2880

gccacuauca caaaguucaa agcuaccggg aagaauaaag agacaggagu gauuguaccu     2940

cgcggcgccc uccaugaaca guccguguau gguaagauca aaguuauuga gaaggacaaa     3000

ccccugaagu accuuuuuga gaacccagau aagaucguca auagucggau aagagaccug     3060

guggaaacua ggcuaagcga gaacgaaaac aauucuaaaa aggcacucuc caccgugaaa     3120

aagaacccua uuuucuuuaa ugaggagaaa aacgaaaucc uggagaaggc gucaugcuau     3180

aaugaugcua cggucuugaa guacaaacug caaaacuuaa aggccaauca gguugacgac     3240

aucguggaug aacgugugaa auuccucgua aaggagcgcc ugacccaguu ugggaguaaa     3300

gaaaaggagg cauucaagga cauucuuugg uuuaaccagg aaaaacaaau acccaucauu     3360

acaguccggc uguucgccag accagaugcu aauaacuugc agaucauuaa gaaagacaag     3420

aauggcaaag auaucggauu ugugcucacu ggcaacaauc accacauagc caucuaugag     3480

gacaaggaua gcaagcuggu ucagcauucg ugugcauucu ggcacgcugu ggagaggaaa     3540

aaguacaaca uucccgccgu caucaaaaau uccgcggacg uguggucucu acugcuugau     3600

aaggaacucc cuguucuguu cuuggacaaa cugccagccg augaguuaaa ccucaaguuu     3660

agcaugcaac agaaugaaau guucauucug gggcuuucac aggagggauu ugacgaagcu     3720

aucaaaaaca acgacaagag ucuguugucc aagcaucucu aucuggugug gucuauaagc     3780

aauaacgauu acuacuuccg acaccaccua gagaccaaaa auacagagcu gaagaacauu     3840

ccgacugcaa aagacuccaa gcgguauuuu cgcuucaaau caguaggugc ccuugucggc     3900

aucaauccca ucaaggugag acucaaccau cugggggaaa uuaccaagau cggagaguga     3960


<210>  2932
<211>  3960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2932
augggcuaca acaucgggcu ggacauugga accgguagcg ugggcugggc cgcucucaca       60

gaugagggga aacuugcaag agccaaagga aagaaucuga uaggcgucag guuguucgac      120

uccgcgcagu cugcugccca acggcgcuca uaucgaacua cgcguagaag guuaagucgg      180

cgcaaaugga gacugaggcu acucgaaaac aucuuuagcg augagauggg uaugauugac      240

gaaaauuucu uugcacggcu gaaguacucc uauguucacc ccaaagauga ggugaacaau      300

gcccauuacu augggggaua ccuuuucccu acccagcagg aaacacacga cuuucaugag      360

aaguuccaga cuaucuauca ucugcgcuug aagcucauga uugaggauug caaauuugac      420

cugcgagaaa ucuaccuggc uaugcaccac auaguaaagu acagaggcca cuuccucaac      480

ucucaaucga aaaugaccau cggcgauuca uauaauccaa gggacuuuca gcaggcaauu      540

cagaacuacg ccgaggcuaa gggguuaauc uggagccuga augaugccca agaaaugaca      600

gacgugcuug ucggacaggc ggguuucggc uugaguaaaa aggcaaaggc cgagcggcug      660

cuauccgcuu uuucuuucga uaccaaagag gacaagaaag ccauucaggc aauccucgcu      720

gggauagugg gaaacacuac agacuuuacc aagauuuuca aucgugaacg cagcggcgau      780

gagcugaaaa aguggaaacu uaagcuggac uccgaagccu ucgaugagca gucacaagcg      840

aucguugacg aauuggauga cgaugagaug gagcucuuua acgccauuag acaggcauuc      900

gacgguuuua cgcugaugga uuuacugggg gaccagacca guaucagcgc ugccauggug      960

aagagguauc aacagcauca cgaugaccuc aaaaugguca aggaaaucgc uaaaaagcag     1020

ggacuuucuc aucaggacuu cuccaaaauu uacacugccu uucugaagga ugacacagau     1080

aagggcauga aagcauugcu agacaaggcc gaucuggcug acgaugugcu cguagagaua     1140

caacagcgga ucgaaucaca cgacuuccug ccgaaacagc gaacuaaggc gaauagcguu     1200

auucccuauc agcuucaccu ggcagaguug gagaaaauca uagaaaacca aggcaaguac     1260

uauccuuuuc uccuggauac cuucacaaau aaggccgggg agaccauuaa caaauuagug     1320

gaacugguca aguuucgcgu gccauacuac gugggaccca uggucacggc ugccgacguu     1380

gagaaagccg guggcgaugc aacuaaccau ugggugaaga gaaaugaagg guaugagaaa     1440

aguccuguaa caccauggaa cuucgaccag guguuuaaua gggaucaggc ugcccaggac     1500

uucaucgacc ggcuaaccgg aacugauaca uaccucaucg gcgaacccac ccugcuuaag     1560

aacucuuuga aguaucaacu guuuacgguc cucaaugagc ugaacaaugu gaaaauuaac     1620

ggucacaaga ucgacgagaa aaccaagcau guucuuauuc aggaucuguu caaauccaag     1680

aaaacaguga gcgaaaaggc aauaaaggac uacuauuugu cgcaggggau gggcgagauc     1740

cagauugucg gacucgcgga uaaaacuaag uucaauucua accuguccuc auacaucgac     1800

uuaaguaaaa ccuuugaugc ugaauuuaug gagaauccug ccaaccaaga gcugcuagaa     1860

aauaucauuc agauacagac aguguuugag gacguaaaga ucgcagaacg cgagcuccaa     1920

aaacuggccc uuccagauga acagguucag caguuggcua agacucacua uaccggcugg     1980

gggaaccuga gcgacaagcu ccuguccaca ccgauuaucc aagagggauc ucagaaagug     2040

agcauuuuaa auaagcugca gaccacuuca aagaacuuca ugaguaucau aacggauaac     2100

aaguuuggug uccagcaaug gauccaggag cagaauacag ccgaaaccgc agacuccauu     2160

caggauagaa ucgacgagcu uacuaccgcu cccgccaaca aaaggggcau uaagcaagcg     2220

uucaaugugu uguuugacau ccagaaggcc augggggagg aaccuaaccg ugucuaccuc     2280

gaguucgcua aagaaacaca gaauagcgug cggaccaacu cucgauacaa uagacugaag     2340

gaucuauaua aaucaaagac ucugagugac gauguuaaag cacucaagga ggaacuggag     2400

agccaaaagu ccucucuuca gucggaaagg auaggagacc gcuuguaccu guauuucuua     2460

cagcagggca aagauaugua cacaggucaa ccaauuaaca ucgacaagcu cagcacggau     2520

uaugacauug aucauaucau accccaggcc uacaccaaag acgauuccau cgacaaucgg     2580

gugcugguau caagaccuga gaacgcuagg aagagugacu cugccacuua uacaaccgaa     2640

guccagcaga gcgcaggggg ccuuuggaaa ucccugaaga augcgggauu cauuucacag     2700

aaaaaguacg aucgcuugac aaagggcggg gacuacagca aaggacaaaa gacugguuuu     2760

aucgcccgac agcucgugga gacccggcag auuaucaaaa acguugcuuc ucugauagag     2820

uccgaguuca gucaaacgaa ggccguggca auucguagcg aaaucaccgc ugauaugaga     2880

aggcuagucg ccaucaaaaa gcaccgcgaa auuaauucuu uucaccaugc auucgacgcc     2940

cugcucauca cagcugcggg ccaguauaug caggcccggu acccagauag agacggggca     3000

aacguguaua augaguucga uuacuauacu aacaccuacc ugaaggagcu uaggcagucc     3060

ucaaguagcu cgcaaguacg acgcuugaaa cccuuuggau ucgugguugg cacaauggcu     3120

aaggguaacg aaaauugguc cgaggacgau acucaguauc ugcggcacgu caugaacuuu     3180

aaaaauauuu uaaccacgag aaggaacgac aaggauaaug gggcccucaa caaagaaaca     3240

auauacgccg uggacccgaa ggcuaagcug aucggcacca auaaaaagcg ucaggaugug     3300

ucucuuuacg gaggcuauau uuacccuuau agcgcauaca ugacucuggu ccgcgccaac     3360

gggaaaaauc uacucguuaa ggugacaauc ucagcggcug agaaaauuaa gaguggacaa     3420

aucgaacugu ccgaguaugu gcagcagcgg cccgagguaa agaaauucga aaagauauug     3480

aucaacaaac uggccauugg ccaguuaguc aauaacgacg guaaucucau cuaccugacc     3540

ucuuaugagu uuuaccauaa cgcaaagcaa cuuuggcugc caacugaaga ggccgacuug     3600

auuagccagc ucaauaaaga uuccucagac gaagaucuga ucaagggguu cgacauacua     3660

accagcccug cuauucugaa gagauuuccc uucuacgagc uugaucucaa aaagcuggug     3720

aacaucaggg acaaauuuau cgcaguugag aacaaguucg auauuuugau ggugauacug     3780

aaagccuuac agcucgacgc ggcucagcaa aagccaguca aaaugaucga uaagaagagu     3840

gccgacugga aagauuaucg acagcgggga ggcauuaagc ugucugacac auccgaaauc     3900

auuuaccaga gcacgaccgg uaucuuugag aaacgcguga agauaucgaa ucuucuguga     3960


<210>  2933
<211>  3963
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2933
augaccgagc agcaaagacc cuacaacauc ggccuggaca uugggacaag cuccauagga       60

ugggccguga cugaucagaa ugacaagcuc cuucacauca ggggucauaa cggcauuggg      120

guccggcugu ucaaagaagg agagucugcu gcagaacgcc gaggcuuucg uacgaccaga      180

aggcgguugu cacgcagaag guggcgguua cgcuuccugg aucgaaucuu ugacgccgag      240

cuagcgaagg uugauccuaa uuuccucgcu agacugaaac agaguaacau uagcccacuu      300

gacccgaaua agcagauguu ugguaacauc cuguucgaug acgcccaauu ugaugaccag      360

aaauuccacc agcaguaucc cacaauauac cauuugaggu ccgcacucgc ccaaccugau      420

gcuccacagg cagacgugcg gcugaucuau cuggcccucc accauaugau uaaguuucgu      480

gggcacuucu uaaauggagc ugccguagaa aaguuucacg gcggccagcu gcaucuugag      540

gcggaguucc aggaauugaa ccaaaucuuu ucuaaucagc agcgcgauca cgugcaacug      600

cagacuucgu cacuagacga ggucgcaacc auucucaaag acaaccagag aacacccagc      660

gauaggcagc gggaacugag ugcccgacuu uucgugccug uugacaagac caucgauaaa      720

gagaauaaga aacuggcuac ugaguugcuc aaggccgugc ugggguuaaa ggcaaaauuc      780

gacgucauac ugggagugga agcuacagau ccacaagccu uuaagcucca guucuccucu      840

gacgauuuug acgauaaaau ugcggagcuu gccagcgaac ugaccgacga ggcaaacgaa      900

aucuugacgg uacuacagcg ccuguacuuc ucccucaauc uggcugauau ucuuacuaac      960

agacagacca agcaaguugc cgacucaauc aguacagcua ugaucaauag guaugacgau     1020

cauaaacacc accugaagau guugaaagag cucgagacuc aggugaccca ggaacagcgg     1080

gacggucuga agaaggccua cgaugaguau auugacggcg auaaacccac aauagacacc     1140

uuuuacacga acgucaagaa aaacuuagau gacagcgaac uggcaaauca aaucaagucu     1200

cuaauugauc uccaggccuu caugccuaaa cagcgcucca aggagaacgg gucaauccca     1260

caucagcugc accaaagaga acuugaccag uugauagagg ugcagggaaa guacuauccg     1320

uggcugcucg cugcgaaaga uaagcuggac gcacuuauug ccuuuagggu gcccuacuau     1380

gucggcccua ugaucgaucc aaaugagagc gacgaccccc aaaacacuaa auucgcuugg     1440

augguucgua aggaagccgg ugagaucaca ccuuggaauu uugaagagaa aguggauaag     1500

accgggagug caacuaacuu cauuaagcgg augacaacca cggacaccua ccuguuggga     1560

gaaccaguac uccccaaaaa uucucuguua uaucagcagu uuacagugcu gaacgagcua     1620

aauaagcuca aagucgaugg caaggcccug acuguggacc agaaaaagua ccuuuaugag     1680

cauguuugca agaaacaacg aagagugacc uugaagaaac uguccgauug ucucauggcu     1740

aggggcgacu ucccgauggg ggccaucuuu acaggaacua ccgaucagaa gaacaugaau     1800

aacacacuga aaaccuacca ggacuuccag gaaauuuucg gugaugagau agacaauccu     1860

gaucgccaag ucgacuuuga aaagaugauc gaguggagca cuauuuucga ggaucgggaa     1920

aucuuuaaau uaaagcuggc agagcuuaac ugguugacgu cggaacagcu cgagagagug     1980

ucuaagaaaa gguaccaggg cugggggcgc cugucccaga agcuacugac acacaucgua     2040

aaugaaaacg gagagcgaau uauagaccaa cucuggaaca ccaaucagaa cuucauggag     2100

aucaugggcc aggacggugu uaaacaagcg auucaggaac auaauggggc uaacguggcc     2160

cagacuggca ucaaugagac cauugaugaa cuguauacau caccccagaa caagaaagca     2220

auccggcaaa uacuuuuggu cguggacgau aucaagaagg ccgucggaua cgcuccagcc     2280

aauauuauga uggaguuugc acgugaagac agagaugacc acaggcugau cgcuagucgc     2340

gcccggcagu uagagaaaac cuaucaggag auuagcaaga acugguucga uaaugaaucc     2400

gugacucagg agcucaaaga ccaaaucaag aacaaaacaa aguuuacgga uagacuguac     2460

cuuuauuuca uacagggcgg gauugaccug uacaccggac acccuuugaa uaucgaucag     2520

cucucuagcu augacauuga ccauauccug ccccagucau uucuacugga uaacagucuc     2580

aauaacaggg uucugacuuc ccaaagcgaa aaucgagugc acaaggagga ccgccuucca     2640

ggugaaacag uagcgaacaa aaugcggccc accuggaaga ugaugagaga gcagggcuug     2700

augacacaga ggaaauacaa caaucugacu uuaaagccug aucagauauc uaaauucaac     2760

aaucgugggc aauuuaucaa ccgccagcuc gucgagacca gacaggugau uaagcuggcc     2820

gcugacauca ugaaucaaaa guaccaggca gaucagacga aaguugugac cgucaaggcc     2880

aaccuuacac aucacaugcg ggaaaaauuc aauuuuuaua agucaaggaa cgugaaugac     2940

uaccaccaug cuuucgaugc cuaucugacu guauucgugg gaaacuggcu acucaaagag     3000

uacccaaagc ugcagccgua uuuuguuuac ggcgacuucg caaaaaccgg uacaaauaac     3060

uugaagaguu uuaauuuccu guaucgauuu gaacgggauc acaaguuaaa cgacaaaggg     3120

gcgauugaua cugacaagga ucaacagcuc ggcuacauga aacgcaucua cagauucaag     3180

aaaaugcugg ucaccaagca gcuugagacg aaugagggag cccuguauaa gcagacaaua     3240

uacaaagcuc ccgccaacga aaagaacacc aaaaagaaua ggagcuugau uaacacuaaa     3300

aagaaucggc cuuccgacau cuauggcggg uacucucaau cggugacagc auauaugagc     3360

cucguguuuu ucaagaacac cuuuaaacug aucaagaugc cccuagucgc ugccaaugca     3420

auuaaaaacu cccugucaag uuaccaugac acuauccuua agauucucgg aucuaaugag     3480

uucaaaguuc ugauaccaaa ggugaagaug aaccaguuga ucaaagaagg ugauagccug     3540

uauaccgugg uaggcgucua caagcacaau gcccagcagu uaauucucuc cgacaacuca     3600

auggggauca uuacaaaacc uauccgagag auaugcgaua gcgaccugga uucuguguuu     3660

aaugaacuug uugacaacau cgauaaguac cugaauuugu auaaaauuga ggacaagcuc     3720

acgaucaagg agaaauucua cgcucugaag aacaaagaau ccaaguucaa auguauuagu     3780

gauauccuag ugggaaccca uugcaaugcg ggcagaagca accugaagau aauuggucuu     3840

ucuuccuauu gggggauguu uucaggcaau aacgacggac ucaaaaucaa cgagaauagu     3900

caacugaucu accagagccc aacuggcuug uucgaacacc gcgucaaggu gucggauuua     3960

uga                                                                   3963


<210>  2934
<211>  3972
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2934
augaaggacu ucuacaucgg ccuggauaug gggaccgaca gcgugggaug ggccgucgcu       60

gaugaggacu auaaccucug caaauuuaag gguaaagcaa uguggggcgu uagacuuuuc      120

gaugaauccg ccacagcggc ugagaggcgg ggguuucgca cugcccgacg uagagcacag      180

aggaagcggc aacgcauucu gugguugcag auguuauuca augaagagau aucuaaaaag      240

gacaucgccu uuuuccagag acugaacgaa ucaaagcuau accucgagga uaaaaguacg      300

aaugugcccu augcuguauu ugcagacgag agcuucaccg auguggacua ccacaagcug      360

uauccuacaa uuuaccaucu uaggaaagaa cugguccagu ccgaugagcc acacgacgug      420

cgguugguuu aucucgcccu gcaucaccug aucaagaacc gcggacauuu ucucuucgaa      480

aaugugggcu cugauuuuga gaaagagucg ucauucgaag ucuuauuuaa gaaccugcaa      540

aauuacauuc uuggugagua cgaauuggcu cuggagugua ucagcccgcu acagauagcc      600

gacauccuca aggacaaaag uaagcgaaaa acuaccaagu ccucugaaau uugcaaacug      660

cuugggauca gcaagaaagg acagcccaga cugaacgcga uuguguugcu cauguguggc      720

caguccguaa agcuggcaga uuuauauggc gacgagaauu acaacaagga ggaaaaauca      780

aguaucagcu ucgagaaggg guaugaagau aaugaggaaa cauacaggga gcugcuccuu      840

gagcgguucg aacugauaga gaaaauuaag gccuuguaug acugggcuau ccuagccgau      900

auucugaaag gagaaaccua caucucuuuu gcaaagguua agacuuauga cgagcaccgu      960

cacgaucucc aacagcugaa ggcuuucgug aaagaacgcu gcagauccga cuacaacgag     1020

aucuuuaggg agacaucaga uaaacuuaau aacuacaccg ccuauugugg uaaguucaaa     1080

gaaaauggcc ggaacggggu cauucugcau agcgcgaacc agacggacuu uugcaaguac     1140

uugaagagua aauucgauaa gcucgacaaa ucuggauaug aggacauguu ugaaaagaua     1200

gagaauggcu ccuucaugcc uaaaaucgug gugaaggaua acggugucau uccaaugcag     1260

guuaaugaga gcgaagugcg agccauccug aagaacgcau cgggguacuu agacuuucug     1320

aauaaccaag auaaagacgg aauaacugua gcugauaaga uucuacagau cuucgaguuu     1380

cgcauccccu auuacguggg cccucucaau guccacucug acaacuccug gcuggugaga     1440

aaagauggca agauuuaucc auggaauuuc aacaaaaucg uugacgugga uaagucagcc     1500

gaagaguuua uuaauaaacu uaccgcuaag uguacauacu ugcccgacag ggaugucaua     1560

ccuaagaaca guauccugua cagccgguau auggugcuca augaacugaa caaucuuaaa     1620

cuggacggga agaaaccaga uguauccuuc aagcaggccg uuuucaacga cuuguuuaug     1680

caucgcagaa aagugacuca aaaggcacuc agggaguaca uuaaggccca gaccggaaca     1740

gagccggaaa ucaccgguau cgacggcgau uucaaagcua acaugcgucc cgcgauugag     1800

cuggcagggu auaauuuauc ugaaccugac aaggaggaaa uaaucaaagc cauuacgauc     1860

uuuggagaug acaagaaacu gcuaaagcgg cgaauuaagg auaaauacag cgagaugcuc     1920

ucagaggacg aaaucaagaa aauaugcaag cugaaauaua aggauugggg cagacuuagu     1980

agggaguucu ugacugaaau cuuugacguc gauacagcua ccggugagug uaaggacaac     2040

auuaucaaua cucuguggaa cacaaaugau aaccucaugg aacuguuagg ggccaaauac     2100

aauuucaccc aguccauuga gcaggcaauc agcaagucua cgcacaaagc cucaauagag     2160

aagauggugg aagacaugua ugucagucca aaagugaagc gcaccguuuu ucaaagccug     2220

aaaauuauga aggagaucgu gaagauuaaa ggccacgcuc ccaagaaagu auucgucgaa     2280

augacacggc augauggaac uaagggcgac aacgggagaa ccaaauccag gaagaagcag     2340

cuugaggagu uguacaaaaa gugcaaagaa gacucuacag agcucuggga ugaacuggag     2400

aagacugacg augacgaauu ucgccaggau aaacuauucc uguacuauac ccagauggga     2460

cgauguaugu acucggguga gcggaucgag cuccgugacc uguuuaauaa gaaccuuuau     2520

gauauagacc acaucuuccc uagaagcaag gugaaagaug acuccauuga uaauaggguu     2580

uuggugaaga aacaaaucaa cucacauaag gacaaugacu acccacugga uaguacaguc     2640

cgcgagaaaa ugcggggcca cuggcaguau uuacucagac auggguuuau uucuaagaag     2700

aaauacgaca ggcugacccg agugacuccc cuuacggaug acgaacuggc cgauuucauc     2760

gcacgccagu ugguagaaac aagccagucc accaaggcgg uggcuacucu ccugaaaacc     2820

cuauauccga caaccgagau aguuuacguc aaggccgaac uggugucacg guucagaacu     2880

gaguacaaau uuauuaagug cagggaggug aacgaccucc accacgcaaa ggaugccuau     2940

cugaauaucg ucguuggaaa cguguacaac gugcguuaua cacauagcaa agcuaucuuc     3000

auuaagggcc uucaaacgaa aaaguacucu uugaaucgca uguuugccuu cgacaccccu     3060

ggugcaugga acaucgaaaa ugagaaaucc cugaguauug uaaagaaaac ugucaugaag     3120

aacaauauau uauauacacg guacagcuau augaagaaag ggggccucuu uaagauccag     3180

ccccugaaaa agggaaacgg ccaggcucca cuuaaaaagg aucugccucu aucugacauu     3240

gggaaguacg gagguuacga uaaacccacc gccuccuauu ucucauacau caaguaugaa     3300

ggcaaaaagg ggaaagagug cagacagcuc guggcgauug acaauuaccu gaagaaguau     3360

uacgaagaga acccaaucga guauuugaca aauaggcugg gacucaaaaa ccccgccguu     3420

uuaauaccug ugaucaagua caauaguugu auugaaaucg auggcuuucg aaugaacauu     3480

agcucgaaau ccaaugaggg uaagacuauc gucuacaaac cagcugugca acugguucuu     3540

ggggcagaaa aggaggccua ugugaagaaa auaucuaagg uacugagcaa accggaggaa     3600

cagaagauua ccucauacga cggcgucagu gcugaggaaa acaucgaguu guucgacgcc     3660

cucacggcaa aaaugaccaa uacaaucuuu aagauuaaac uggaagauau aggaaacaag     3720

aucaaguccg gccgggacaa auucgugacu cuagauguga agaaacagug cuaugucauu     3780

aacgagaucc ugaagauuau gcacuguaau guugugaccg gggaccuuuc ucucaucgga     3840

gcggccaaaa aggcuggcau aguaagcaca aacuccgccc ugucagagau caagaaagug     3900

agcaguauua uguugaucaa ucaaucuguc acuggucugu uugaaaccaa gauugauuua     3960

cucaacaucu ga                                                         3972


<210>  2935
<211>  3975
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2935
augagagcca agggcaaaac ccugaucggg acaagccucu ucagggaggg aaacacugcu       60

gcagaacggc gcgguuaccg aacgacccag cguagacuua agaggcggaa acgcagacug      120

aaguuguuag aggccuuuuu cgaccccuau auuuuugaag uggaugagaa uuuccuggcg      180

aggcuacacg aagcugacau auccccuaaa gaugagaaca agcgguuuug gggcucucuc      240

cuguucccaa augagaagga cgccaacuuu uaccgcaaau auccgacaau cuaccaucuu      300

cgaaaggcac ugauggaaga ggauagaaaa uucgacguca gggccauuuu cuuggcuauc      360

caccauauua ugaaguaucg ggggaauuuc cucucacccc ugaguguuga uagcuuuaaa      420

caagaaggac uggagcucaa ggagcaguua gaccgucagc ugaagcgcgu ggcagaacuu      480

uacaacgagu ugaauacuga uguaaaaauc aacguguccg acgucgaaac cauacaggau      540

aucauuaucg acaagaauaa aucuagaagg gauaaggccg gcgcucugaa aaagcuacuc      600

gccgugucgu cagaccggaa agagauuaac aagcugaaug cgagccuugc aaaggaacug      660

aguaacgcca ucuuggguau gaaagcucaa uucgccaauc uccuguuagc agacgaucug      720

gagauagaca agaacaaaaa gaauuuuucc cuccuugaua caaacgcuga ggaaaacguu      780

ucugggcugu uggagcuagu gaaugaaaaa caggccgagc uguucgacgc gauuaagacc      840

auccacgauc agauuauccu caacgaaauc gucccugacg gaaagagcau uucccaguca      900

augguggaua aauaugaggc ccaucgagag caacugcagg uacuuaaggc acuguacgag      960

aaaauggacg cuaagcagau aaaagccauc aagaaggcuu acaguuugua uauugauggc     1020

aaagacccaa aggccaaaaa gguugauaaa cgcgcacacc agaauagagc cgacuucuac     1080

aaggaaguga agaaaguccu cguggaacug gacgauagca gggcugagaa guuacugggc     1140

cuaaucgaaa uggggcaauu ucuccccaaa cagcggacug cgaugaacgg agugauaccu     1200

aaucagcugc accaagucga gcuugacaag auuaucgaga ugcagucuaa auauuaccca     1260

ugguuggcag aaaagaaccc cguuaaaucc caucagaagc gcgccccuua uaagcuggau     1320

gagcuccuga cauucagagu gccauacuau guagguccga uggugaccag uaaaaagcag     1380

caacagacga gcaauaguaa cuuugcuugg auggucagga aacaggaagg cgagaucacu     1440

cccuggaauu ucgaggaaaa gguggacacc auugccucug caaacgauuu uaucaaacgu     1500

augacagcca aggacacuua ccuuguuggg gaggaugugc ugccugcuaa uuccuugcuc     1560

uaccagcggu auaagguccu gaacgaauua aauaacauua aagugaaugg acgaagacug     1620

ccaguagagc uaaagaggga agccuacguu gcacucuucg agaacggcga cagcaaaacc     1680

gugucggcga agugcuuuaa aaacuggcug aaugucaagu ugaagaaccu uaauucucug     1740

aaacucgagg gucuguccac agggaccaag uucgauaaca auuuaucaac guaucacgac     1800

cugaaaaaga uauuuggaaa agcuguggau gacgccggcc gccaaaagga uuucgaacag     1860

aucauugagu ggcuuacuau cuuugaagac aagaaaaucu ugcggauuaa gcucaaagag     1920

auagaauggc ugacagagga gaacaucacc cagcuaacua gacugaauua caacggcugg     1980

gggaggcuca gucgccgacu gcuuacagga auuugugaca gcaaugguca gcggaucaug     2040

gauugguugu gggacaccac gaacaauuuc augcaaauuc agucccagcc cgauuuucag     2100

aaggcaaucg ucgaauauaa cucugacaaa cuggagcaag gcgauauaga acagaucuua     2160

ggggaggccu acaccagccc ucagaauaag aaagcuauuc guaaggugau caagguugug     2220

caagacguag aaaaagccau gggagcaaag ccaacaucaa uuaguaucga guucacuaga     2280

uccgauggca ggagcgagcu caccaacucu cgcaugacac agcugcagaa aacucuuaug     2340

ucacugagug aagagaugac cgaccaggcu uugcucgccg aauuuaagga uuuucaagcg     2400

agcaaaaaga agcugacaaa uaaauauuac cuauacuuca cccagcuggg ucgggacaug     2460

uauacugggg agccccucaa cauagaucag cugucccuuu acucugacgg cgaucauauu     2520

uugccgagau cguuuaucaa ggacgacagc cuggauaaca gggucuuaac gaaacgagcc     2580

gugaauaacg agaaguccga cacaguuccu gugugcgaau auggagcuaa aaugaaggau     2640

uucuggaaug accucuacga uaacggccug auuucaaagc agaaacuugc aaaucugcua     2700

accgaccccg augagaucgg gccauauaag gccaacggau uuauacgccg gcaacucguc     2760

gaaacuucac aggugaucaa acugucugcu gagauuuugc agacccugua ccaggacaca     2820

aagaucauug aaguaagaca aaaauauacc cacgaguuaa gggagcacuu caauuuugug     2880

aagauccguc agcucaacga uuaccaucac gccuucgacg cauaccugac ugcguuuagc     2940

ggucgcuauc uuuaccagcg guauccuaag cugagaggcu acuucguuua uggggauuac     3000

aauaaauuug acgccaagaa agacaggaac uauuugaagu ccuacaauuu ccucuacgau     3060

cuggaagagg gaaaaacaga ccaaauaguc gauaacgugg aacauaaggu guuuaugaau     3120

cgacaggacg cuauuaacca gcuaaagaaa gccuaugagu acaaguauau gcuggucuca     3180

caggaaacgu acaccaauag cggcgcaaug uucaaagaga cuaucuaucc cguuaaccca     3240

aagcaaaaac uuauccagaa gaaaaacgau aagcccgugg acauuuacgg uggguauucu     3300

aauaacuacg augcuuaccu cuccaucgug cggcuggagg acaagaaagg cauuaaguuc     3360

cgcguagucg gaauaccuau gagaaauaaa gccgaauugg auaaccuggc aaaggaggac     3420

cacaauggcu auaugcagag guuaaacgaa aucguggaug agcagauuaa uaguagcggg     3480

aaaaagaaga cauuucggau cauucuccca aaaguucauu ucaagcaacu gguggaagac     3540

gauucuggaa aauuuaugcu ugguuccgcc gcuuacccgu auaacgcgaa gcagcugguc     3600

uugucaguga aaaguaucga gagccucgcc aauaaggacg caucggaccg agagcugguu     3660

gcuguguacg aagagauacu aaacguaguc aaugauaagu ucacccugua ugacgccaga     3720

caguuucgca acaaacuuac auccucuaag gauaaauucg uggaacucaa uacugccgac     3780

aaggugaccg agcuguugga uaucuuagac ggccugcacg cuaaccccgu cguuaaaaag     3840

cucaagauua ucagcggguc accuuuugga uuccugcaga ggccaagugg cauugugcuu     3900

acggagaaug caaaacugau cuaccaaucc cccaccggua uauuugaacg uguaguguau     3960

uugcgggauc uauga                                                      3975


<210>  2936
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2936
auggagggcc agaugaagaa caaugggaac aaucugcaac agggaaacua cuaucucggu       60

cuugacgugg gcaccagcuc cgucgggugg gccguuacag auacugacua caaugugcug      120

aaauucagag gaaagucuau guggggcgcu agguuguuug augaagcauc aacggccgag      180

gaacggcgca cccaccgagg uaaccguaga agguuagcgc ggcgcaaaua uagacugcua      240

cuccuggagc agcuuuucga aaaggagauc aggaaaauug acgauaauuu cuucguacgg      300

cugcaugaga guaacuugug ggcugacgau aagagcaagc ccuccaaauu ucuccuguuc      360

aaugacacaa acuuuacuga uaaggacuac cugaaaaagu auccuaccau auaccaccuc      420

cgcucugauu uaauccauaa uucgacagaa cacgacauuc gacugguguu ccuugccuug      480

caucaccuga ucaaauauag agggcacuuu auuuacgaua acucagcaaa uggagacguc      540

aagacccuag acgaggccgu gagcgauuuc gaagaguacc ucaacgagaa ugacaucgaa      600

uuuaacauag agaacaagaa agaauuuauc aauguucuga gugauaagca ucuuacuaaa      660

aaggagaaaa agauuucccu gaaaaaguug uauggcgaca ucacagauuc ugaaaacauu      720

aauaucagcg ugcucauaga gaugcugucc ggcucaagua uuagcuuauc uaaccuguuu      780

aaggacaucg aguucgaugg gaaacagaau cucucccuug acucagauau ugaagagacc      840

cugaacgacg ucguggauau cuugggagac aauaucgacc uacugauuca cgcuaaggaa      900

guauacgaua uagcaguucu cacgagcagu cuggguaaac auaaguaucu uugcgacgcc      960

aaaguggagc uguucgaaaa gaacaagaaa gauuugauga uccucaagaa auacauuaag     1020

aaaaaucacc cagaggacua uaagaagauc uuuucuuccc cgacugagaa aaagaacuac     1080

gcugccuaua gccaaaccaa uucgaaaaac gucuguucuc aggaagaguu cugccuguuu     1140

auaaagcccu acauuaggga uauggugaaa uccgaaaaug aggacgaagu gcggaucgcg     1200

aaggaggucg aggauaaguc auucuuaaca aaacugaagg gcacuaacaa uaguguugug     1260

ccuuaccaga uccacgaacg ugagcuaaac cagauucuca aaaacaucgu agcauaucug     1320

ccauuuauga augacgaaca agaggauauu agcguggucg acaagauaaa acuuaucuuc     1380

aaguuuaaaa uucccuacua uguggggccu uugaacacca aguccacacg cucuuggguu     1440

uacagaagcg augagaagau cuauccaugg aauuucucaa acgugaucga ccuggauaaa     1500

accgcccaug aauuuaugaa uaggcucauu ggacggugua cguacacuaa cgaccccguc     1560

cugccuaugg acagucuucu guauuccaag uacaaugugu ugaacgagau aaauccaauc     1620

aaaguaaacg gcaaggcuau uccgguugaa gugaaacagg ccaucuacac agaucucuuc     1680

gagaauagca agaagaaagu cacccgaaag ucuauuuaua ucuaccugcu gaaaaacggu     1740

uauauagaaa aggaggacau cgugucaggg auugauaucg agauuaaaag uaaguuaaag     1800

agccaccaug acuuuacuca gaucguccag gaaaauaaau gcacacccga ggaaauagag     1860

cgcauuauca agggcauucu aguguacucc gaugacaaau cuaugcucag aagguggcug     1920

aagaacaaua ucaaaggacu uucggaaaac gauguuaagu auuuggcaaa gcugaacuac     1980

aaagaguggg gccggcucag caagacccug uuaacggaca uauauaccau caauccugag     2040

gauggggagg cuuguuccau ucuggacauc auguggaaca caaaugccac ucuuauggaa     2100

auuuugucaa acgaaaaaua ccaauucaag cagaauaucg agaacuacaa agcggaaaau     2160

uaugaugaga agcagaaccu ccacgaggaa cuggacgaua uguacauaag uccagccgca     2220

cgcagaucua uuuggcaagc ucuaaggauc guggacgaga ucguagacau uaaaaagagc     2280

gcccccaaga aaaucuuuau ugaaauggcu cgugagaaga aauccgccau gaagaaaaag     2340

cggaccgagu cacgaaagga uacacugcuc gaacuguaua aaagcugcaa gucucaggca     2400

gacggauucu acgaugagga acuuuucgag aaauugucca augaaaguaa cagcagacug     2460

aggcgcgacc aguuauaucu cuacuauacu cagauggguc ggucuaugua caccggcaag     2520

agaauagauu uugacaaacu gaucaaugau aagaacacau acgacauuga ucauaucuau     2580

ccuaggucca agauuaaaga cgauucaauc accaaucgcg uccuugugga gaaggacaua     2640

aacggggaga aaacugacau cuacccaauu agugaagaua uccgacaaaa gaugcagccc     2700

uucuggaaaa uucugaagga gaagggauug aucaaugaag agaaauauaa gcggcucacg     2760

cguaacuacg aacugacaga cgaggagcua agcucguuug uugccagaca gcugguggaa     2820

acccagcaau ccacuaaagc ucucgcgacc cugcuuaaga aagaguaucc gucugcaaag     2880

auagucuaca gcaaggccgg caacguguca gaguucagga aucgcaaaga uaaggaauug     2940

ccuaaauuuc gggaaauuaa cgaccugcac cacgcuaagg augccuauuu aaauaucgua     3000

guggguaacg uuuacgacac aaaauucacc gagaaguuuu ucaauaacau cagaaaugag     3060

aacuacaguc ucaaaagggu cuuugauuuc uccgugcccg gggcauggga cgccaagggc     3120

ucuacuuuua auacaauuaa gaaauauaug gcuaagaaca auccaauaau cgccuucgca     3180

ccuuacgaag ugaaaggaga gcuguuugau cagcagauug uccccaaggg caaagggcag     3240

uucccaauca agcaaggaaa ggacauugaa aaauauggug gcuacaacaa acuuagcucc     3300

gcguuccugu uugcuguuga guauaagggg aagaaagccc gagagcgcuc acuagaaacg     3360

guguacauca aggaugugga gcucuaucug caggacccca uaaaguacug ugaaagcgua     3420

uugggacuga aagagccuca gaucauuaag ccaaaaaucu uaaugggcag ucucuucucu     3480

auuaauaaca agaaacuggu cgugaccggu cgguccggga agcaguacgu uugccaucac     3540

aucuaucaac uuagcauaaa ugaugaagac ucgcaguacc ugaagaacau ugcaaaauau     3600

uugcaggagg agccggacgg caauaucgaa agacaaaaca uccucaacau uacuucagug     3660

aauaacauca agcuguuuga uguccuaugu acaaaauuca auucuaacac cuacgagaua     3720

auucugaaua gucuuaagaa cgacgugaau gaaggaaggg agaaauuuuc cgaacucgau     3780

auccuggagc agugcaacau uuugcugcag uuacucaagg ccuucaaaug uaaucgugag     3840

agcucuaacc uggaaaaacu uaauaacaag aagcaggcug gcguuaucgu gauaccccau     3900

cuguuuacaa agugcagcgu auucaaaguc auucaccaau ccaucacugg guuguuugag     3960

aaggaaaugg accuccugaa auga                                            3984


<210>  2937
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2937
augaagaacu acagcaucgg ccuggacauu gggaccggau ccguggguug ggucugcaua       60

gaugagaauu aucagauccu cagauacaac aauagggccg cuuucggcgu ucacgaauuu      120

gagacagcac auguggccga ggagcggcgc auuaaacgag gggcgcguag aagguauaac      180

cggcgcaaga aaagaaucca acuucugcag ucuuuguucg uaccccacau gcagcagaag      240

gcguuuuucg ccccuacuau uacggaacau uacuggaaaa augacaacca auuugagaau      300

aggaccuuau cagaggugcu gaugagucua ggaaugaacc caaauacaua uccgacuauc      360

uuccaccucc ggcaugaacu gauagagagc uccgucaaga aggaugcacg ccuuaucuac      420

cuggccuugc acaaccucgu gaaauuucga ggccacuucc ugcaggaagg uucuaccugg      480

aaugcuaaca ugucggacgc aaaucuguca gagcaguuug agcagcucuu acauauuuau      540

acagaaaugg aggaacugcc ccaaguuagc cuuaccgccc agcagaguac ugauguggag      600

cacaucuugc ugaacaauca uauuuccaag gcugaaagag ccaggcagcu aacagcucuc      660

uucgagcgga ccuacgagcc auuuuucaaa cugaucgucg ggcuugacau agcagccgcu      720

caacuguuua agcagucuga acagcaauug cucuacaaag agagcaagac gaaggugaac      780

cugacugccg aaaccuuacc ugagguacug cagacaaugc ucgaacccga gguugcauuc      840

auugaggcug ugcagccuuu cuaucagcaa acuguccuuu uugaucuguu gaaaggacag      900

uccuacgugg ccgcggccaa gguggaagca uucaaccagu augagcguga ccuagcucug      960

cucaaaucag ucaucaauga aguuggcagu gagaagcagu accgcgccuu uuucauuacc     1020

agcaaacaaa agaaaagaca guuugaacag aagccaaaca uggagcugcu uugucaccug     1080

gauagguuca aucgggagaa gaaauaugaa gagacauuuu acaaggaagu gucuaaauug     1140

cucaccgcuc ugaucaagga ggccacgggc gagguaaaag aacacaucga gcagauuaua     1200

caagcaaugg aaucccaguc auucuuagug aagcagaagg ggauccagaa cgccagcauu     1260

cccuaucaaa auagucugua cgaggcugaa augauccuac gaaaccagca ggcguacuau     1320

ccuuggauaa cugacgaguu uauugagaaa gucaagcaac ucaucgcauu ccgcauccca     1380

uacuauauug gaccgcuggu gcagggugau gaacagcccu uuggcuggcu ucagagacaa     1440

gccgacggga auaucacacc uuggaacuuc gaugagaaag uuaauaaggc ugccucugca     1500

gaauccuuua uuaccaggau gacuaaaaac ugcacauacu ugaaggagga aaaggugcug     1560

ccaaaacaga gccucaugua ugagaaguuc acccugcuua augagcugaa cucgguccag     1620

auacggucuu ccuuggaaca gccccauaaa aagcaccgcc ucugugagga caugaaacaa     1680

uggaucauug aacauguguu caagaagacg aaaaccguaa cacacaagac ucugauccag     1740

gcccugaaaa agucaccuua caaagacgag uuauaugaug aaaguaccgg aaauuggaag     1800

gacguuuacg gcacacagaa agaggauaga uuugcuagcu cccuaucuac uuacaucagc     1860

cucaaggaga uuguggguga cauagucgau aacgccuuca uggaagagau cauuuauugg     1920

cugaccgugu uuacagacag ggauaucauu acccuuaaga ugaaugacaa auacccauuc     1980

aucacugaac agcaaauaca gcguuugcug ucacucaaca ucagugggug gggacagcug     2040

ucccaacggu uacugcagag cauucccguc gaugacauga accgaucugu gaucgaguau     2100

auggagaagc augcaguuaa uuuuauggaa cuuuugucac cgagacagag ugaucucgcg     2160

uacaaaauug aacaggugaa caagcaacac agcaaaaaga uccacaagau aagguaugaa     2220

gacauugagc agcugacggg cuccccugcu cuaaaacgcg gcaucuggcg gucuguaaag     2280

auuaucgagg aacugacagc cauauucggg gagcccaaaa auaucaugau ugaagucgca     2340

agagaggacc aggccucgca uaggaccaag agccgcaaag aacaguggga guccaucgga     2400

aagucacucg cuaaggaaga gaaagagcug aaggccuuuu ucaaagaaau ggcacaauac     2460

ccaaagcagc aguuugaucg aacucgguuc uggcuuuaug ugacccagca agguaaaugc     2520

uuguacacag gcaagacccu gcguuuagag gaccucaguu cuuacgaaau ugaucacauc     2580

cugccuagaa acuuuguuaa ggacgauagc auagacaauc uugcucuggu guaucccaug     2640

gccaaccagg agaaaaauaa ggcggggaac aauaaaaugc cauuggaaau uaucgaugcc     2700

aagcagcagc ccgcuaugau ggcacauugg aaaaggcuca accaacugaa gcuaaucucc     2760

gacucaaaac ugcacaagcu caugaagccu agcuucaaug augucgacaa agagaacuuu     2820

auugcccgcc agcuggugga gacucggcag aucauuaagc acguaagaga ucuuuugcaa     2880

gaaagguucg agcauacaga cauacacgug guuaaagcua agauggucuc ucaguuccga     2940

aaauuuuccc gcaucccaaa gauucgggau uacaauaaca agcaucacgc cauggacgca     3000

cuguucacga ccguguuaau ccaguuuauu cuccagaaau auggaaagaa uuuccugcaa     3060

uuugaccuuc agcauaaaga uaucaguaag aaauggagca agauagcgca gucuacuaag     3120

cacuucuuuc uguuccagca auuugccgaa cagcagauca aauccccggu gacaaaggag     3180

cuagucucag gcguucagua cuucgagcac aucuuuuaug aacaaaguug gcagaccaca     3240

aaaaugacuc agaccauuga cagcaacuuc uacaaggaga cgauuuucuc gcccaaacuc     3300

aagaccgcua aguauacauc cgccaaagca gaaaaguuug ugcaugaugc uaucgugaaa     3360

uacgccguau gugguauaca auacacuucu cugaagaaaa acaaggagca gcaccauguc     3420

uugcugaccg acauuaaugu gaucgaacac uaucaguuaa aggaggcaag ccucaacgag     3480

cuggcccuuc aucuggcuaa aagagaauca aaggaugacg uuaucgaugc gcagauguug     3540

guggucauuc cuaaauacaa gaaaauaaug cucaauggga guccauauua cugcaucucc     3600

ucugacgaga ggcacauugc cacacaauuc gugcugcgua aggaucuacu ggaagcaguu     3660

cuucacguga acgacgagac uagcguagau gaacuccgca cccuguuucg guccaucguc     3720

gaggagauga ugacgcagua uaaaguguac aauaaggaca caauuagaaa caagaucgau     3780

gcuuucauag aaaccgaguu gaucgacgug gaaacuuuuc aggucggcuu cacagagauu     3840

aaaaagaugg uugccgccaa ugcucagagg ucagacaaau uuggaggccg acugcggcaa     3900

gugaugaagg uagaagauau cuauauuauc gacgagagca uaaccggguu acgcuacaga     3960

aaacccagga agcucgugaa guga                                            3984


<210>  2938
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2938
augaagaaaa acuacaccau cggccuggac auugggacag ccagcguggg augggcuguc       60

cucacugagg auuaugaacu uauaaagaga aaaaugaaga ucuccgguaa uacgcagaaa      120

aaggcaguua agaaaaacuu cuggggcgug aggcuguuug agcaagggga aaccgccgag      180

ggacggcgcu ugaagcgaac aacucguaga aggauugcgc ggcgcagaca gaggauccag      240

uacuuacgga ccauuuucga cgaaggcaug aaucagguag augcuaacuu uuucgcccgc      300

cuggacgagu cuuuuucaau cauagaugag aaagaaaaug agcgacaccc caucuucggu      360

aacguggcag aagaggccgc uuaucaugag caauuuccua caauuuacca ccuaagagaa      420

caucucgcaa auaguagcga gcaggccgac cugaggcuug ucuaucuggc uuuggcccac      480

aucauuaagu uccgggggca uuuucucauc gaaggagagc ugaacaccga aaauuccucu      540

gugucgggca cuuucgagca guuuauaaaa guuuacaacg agacauucaa uguggaaaag      600

gcgcuggauc ucaccgucga cuuagauggc auugcagagc agaagaucuc acguaugaaa      660

cgcgccgaac ugauucuuag cuuguuucca gaggaaaaga guacggggga cuucgcucaa      720

uucaucaaaa ugaucguggg aaaccagggu aauguaaaga aaacuuuuuc ccugaacgag      780

gaugccaaga uucaguucuc uaaagaggaa uaugaggaaa aucuagagac ccuccuggca      840

gaaauaggcg aggacuuuag aagcguuuuc gaugcugcca aguccgugua cgacgcgauc      900

ucacuugcca acauucugaa ggucacagac gcaacuacca gggcuaaauu gaguagcucu      960

augguggugc gguuuacaga ucacaaggag gaccucaaaa cccugaagcg auucguccgc     1020

gaaaacuuac cggaugagua cgacgaucug uuuaaaaaua agaagguugc cggguaugcu     1080

ggauacaucg acggcaacga aacgcaggag gccuucuaua aauaccucaa gaaaacucuu     1140

gcaaaggcca caggugcuga gggguuucug gcgaaaaugg aacaagagga uuucuugaga     1200

aagcagagga ccuuugacaa uggagugaua cccuaucagc uacaccugga agaacucaag     1260

gcaauuauca aaaaccaaaa guccuacuau ucauuccugg augaagagaa aaucagccag     1320

cuuaugacuu uucggauucc uuacuacgua ggcccacugg ccaaggagaa aggccaguuc     1380

gcuugguuga cacgcaagga aauggggaag aucacccccu ggaaucucaa cgagaaagug     1440

gacauugaaa agagugccac ggauuucguc gagagaauga ccaauaacga cucuuaucug     1500

ccuauggaaa aaguguuacc aaagcauucc cugcuauacg agacauuuac uguuuauaau     1560

gagcucacca aagugaggua cauagacgau aacggacgug cacagaauuu cagcucgaag     1620

gaaaaacggc aaaucauuaa cgagcuguuu aagcagcagc gaaaggucaa aaaggaaaug     1680

cuugaggccu ucuugaaaaa ugaauauggu aucgagaacc ccaaggugga gggcaucgaa     1740

aaaucuuuua augcuugccu ggggacauac cacgaccuca uuaagcuggg aauaagaccu     1800

gagcuuuucg aacagccaga gcaugagcaa caguuugaac agaucguaaa gauucugacu     1860

guuuucgagg auaggaaaau gcgccggaag caguuggaaa aauuuuccaa cauccucacc     1920

gaggaggagc aaaagcagcu ggagagaaaa cacuauaagg gcugggguag guuaucagcc     1980

aagcugauuc acgggaucgu ggaccagaaa acacagaaga ccauacuaga uuaccucauc     2040

gacgaugacg aucugccgaa aaaccgcaau cgaaacuuca ugcaacuuau uaaugacgaa     2100

aacuugaguu uuaaggagga aaucgagaaa auugcauucg auaaugacaa gagcacugaa     2160

gagaucgucc aggagcuggc gggcuccccc gcuauaaaga aaggcauucu ccagucucug     2220

aagaucgugg aagagauuau cgauauaaug ggagaauuac cuacgaacau cgucguggag     2280

auggcccggg aaaaucaaac aaccgcacag gggaaccgug ccagcaaagc uagaaugaag     2340

uaccuggagg agucaauuaa aaagcuggga aguuccaucu uggaagacga gccaauuagc     2400

aaagacgcca aucuccugag gaacgaucgc cuauuucugu auuaccucca gaauggucgg     2460

gacauguaua cuggcaacga acuggauauc aauaaccuuu cuucauacga cauagaucau     2520

auuguucccc agaguuucgu gaaggacgau agcaucgaca auagaguauu gaccacaucc     2580

ucuaugaaca gggggaaguc gaacaccguc ccugcagaga gcgugguuaa aaagaugcga     2640

ccaacuuggg agcgccuguu agcuuccgga cucaucucaa agaagaaauu caguuaucug     2700

acaaaggcca cgaauggcgg ucuuaccgaa gaggauaagg cgcacuuuau ucaacggcag     2760

cugguggaaa cuagacagau caccaaaaac gucgcccaga uuuugcauca aaaguacaau     2820

aacgagcagc ucucugaaaa acccgugagg guaauaacac uuaagagcgc ucuagcaucc     2880

caguuccgua aagacuuuuc acuguauaag auccgcgagc ucaaugauua ccaccaugcc     2940

caggacgcuu accugaacgg ggugauugcc caagcacuuu ugaagguuua uccgaaacug     3000

gagccugagu ucgucuacgg cgaauaucag aaggugagca uccgggcguu aaauaaagcc     3060

acagcuaaga aagaauuuua cucuaacauu augaauuucu uuacuuccga ugagauggug     3120

gccaacgaag agaccggaga gguccucugg aauagacaga gggacaucaa gacgauaaag     3180

aaaguuauga acuaucacca aaugaauauc gugaagaaag uggaaauuca gaccggcgca     3240

uucacaaagg agaguauccu gcccaaaggg ccaagcaaga aacuuauugc ucggaaaaac     3300

aauugggccc cuguaaacua cggagguguc gacucuccca cuguggcaua uuccguuauc     3360

auaacccacg aaaagggcaa agccgcuaag gugguccagc agcugguggg gauuaaaauc     3420

cuagagcgac aagcguuuga acaggaugag guugccuucc ucgaggaaaa gggauuuauc     3480

cauccaaaag ugcagcugaa guugcccaag uacucacugu accaguucgc agacggccgc     3540

agaagguuac ucgcuagugc cgaggaaagc caaaaaggua accagauggu acugccuguc     3600

caccuuauug agcuguugua ucaugccaag cacgugucgg auuccucugg gaaaagccuc     3660

gaguaccuga augaacaccg gcaugaguuc gcugaacuac uggaggcaau acuucaguuu     3720

acagaacagu auaucgacgc cggcaagaac cagaaaaagg ugcgagaucu cuacgagaag     3780

aaucaagacg cggauaugag agagcuggcu ucaaguuuca uucaguugcu gcaguuaaac     3840

aaacaaggag ccccagcaga cuuuaaguuc uuuggcgaaa cuaucccgcg caggcguuau     3900

aaaaauaccg ccgagauugu cgaugcuacg uucaucaacc aguccauaac agggcucuac     3960

gaaacccaga agcggcuggu uuga                                            3984


<210>  2939
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2939
augaccgagc agcaacagcc cuacaacauc ggccuggaca uugggacaag cuccauagga       60

ugggccguga cugaucagaa ugacaagcuc cuucacauca gaggucauaa cggcauuggg      120

gucaggcugu ucaaagaagg agagucugcu gcagaacggc gcggcuuucg aacgacccgu      180

agaagguugu cacggcgcag auggagguua cgguuccugg aucgcaucuu ugacgccgag      240

cuagcgaagg uugauccuaa uuuccucgcu cgacugaaac agaguaacau uagcccacuu      300

gacccgaaua agcaaauguu ugguaacauc cuguucgaug acccccaguu ugaugaccag      360

aaauuccacc agcaauaucc uacaauauac cauuugagau ccgcccucgc acagccagau      420

gcuccccagg ccgacgugag gcugaucuau cuggcacucc accauaugau uaaguuucgg      480

gggcacuucu uaaauggagc cgcuguagaa aaguuucacg gcggccagcu gcaucuugag      540

ucugaguucc aagaauugaa ccagaucuuu ucgaaucagc aacgugauca cgugcagcug      600

cagacuucaa gccuagacga ggucgccacc auucucaaag acaaccagcg cacaccuagu      660

gauagacaaa gggaacuguc cgcgcggcuu uucgugccag uugacaagac caucgauaaa      720

gagaauaaga aacuggcaac ugaguugcuc aaggccgugc ugggguuaaa ggcuaaauuc      780

gacgucauac ugggagugga agccacagau ccccaggcau uuaagcucca guucucuagc      840

gacgauuuug acgauaaaau ugcugagcuu gccuccgaac ugaccgacga ggcgaacgaa      900

aucuugacgg uacuacagcg acuguacuuc ucacucaauc uggccgauau ucuuacuaac      960

cgccaaacca agcagguugc agacaguauc agcacagcua ugaucaauag auaugacgau     1020

cauaaacacc accugaagau guugaaagag cucgaggccc aggugacuca ggaacaaagg     1080

gacggucuga agaaggcuua caacgaguau auugauggcg acaaaccuac cauagauaca     1140

uuuuacacca acgucaagaa aaauuuagac gauucugaac uggccaacca gaucaagucc     1200

cuaauugacc uccaggcauu caugccaaaa cagcggucaa aggagaaugg gagcaucccg     1260

caucaacugc accagcgcga acuugaucag uugauagagg ugcaaggaaa guacuauccc     1320

uggcugcucg ccgcuaaaga caagcuggau gcgcuuauug cauuuagagu gccuuacuau     1380

gucggcccaa ugaucgaccc caaccagagu gaugacccuc agaauacgaa auucgccugg     1440

augguuagga aggaggcugg ugaaaucacu ccauggaacu uugaggaaaa aguggacaag     1500

acaucuuccg ccaccaauuu cauuaagcgu augacuacaa ccgauacgua ccuguugggg     1560

gagcccguac ucccgaaaaa cagccuguua uaucagcaau uuaccgugcu gaaugaacua     1620

aacaagcuca aagucaaugg aaaggcacug acaguggacc agaaaaagua ccuuuaugau     1680

cauguuugca agaaacagcg gcgagugacu uugaagaaac ugucggacug ucucgucgcc     1740

agaggcgauu ucccuauggg cgcuaucuuu accgggacaa cugaccagaa gaacaugaau     1800

aacacccuga aaacauacca agauuuccag gagauuuucg gagacgagau agauaauccc     1860

gaccgccagg uggauuuuga aaagaugauc gaguggucua ccauuuucga agacagggag     1920

aucuuuaaau uaaagcuggc cgagcuuaac ugguugacuu ccgaacagcu cgagcgggua     1980

ucaaagaaaa gauaccaagg uuggggcagg cugagucaga agcuacugac gcacaucguu     2040

aacgaaaaug gggagcgcau uauagaucag cucuggaaca caaaucaaaa cuucauggaa     2100

aucaugggac aggacagcgu gaaacaggca auucaggagc auaauggcgc gaacgucgcu     2160

caaaccggua ucaaugagac uauugacgaa cuguauaccu ccccacagaa caagaaagcc     2220

auccgacaga uacuuuuggu ggucgaugac aucaagaagg caguggggua cgccccugcu     2280

aauauuauga uggaguuugc ccgggaagau cgugacgauc acagacugau cgcaucuagg     2340

gcucgccagu uagagaaaac auaucaagaa auuagcaaga acugguucga caaugaguca     2400

guuacccagg agcucaaaga ucagaucaag aacaaaacua aguuuacaga ccggcuguac     2460

cuuuauuuca uacagggcgg aauugaucug uacacgggcc accccuugaa uaucgaccaa     2520

cucaguuccu augauauuga ccauauccug ccacagagcu uucuacugga caacucucuc     2580

aauaacagag ugcugaccuc acaggccgaa aacaggguac acaaggagga ucgacuuccc     2640

ggggaaacug ucgcgaauaa aaugcgcccu acauggaaga ugaugcggga gcagggauug     2700

augacccaaa gaaaauacaa caaucugaca uuaaagccag accagauaag uaaauucaac     2760

aauagggguc aguuuaucaa ccgucaacuc guggaaacuc gccagguuau uaagcuggcc     2820

gcugauauca ugagccagaa guaccaggca gaccaaacca aaguggucac ggugaaggcc     2880

aaucuuaccc aucacaugcg ggagaaauuc aacuuuuaua aguccagaaa uguaaacgau     2940

uaccaccaug cuuucgacgc cuaucugaca cuauucgugg gcaauuggcu ccugaaagag     3000

uacccgaagu ugcagcccua uuuuguuuac ggggauuucg caaaaacugg aaccaacaau     3060

cugaagucuu uuaacuucuu auauagguuu gaacgagacc acaagcucaa ugauaaaggc     3120

gcgauugaca cagauaagga ccagcagcug gguuacauga aacggaucua ccgcuucaag     3180

aaaaugcuug ucacuaagca acuggagacc aacgaagggg ccuuguaugg ccagacgcuc     3240

uacccugcuc caaagaacga uaaaaagaca agaaaucuga uaaacucgaa aaagaauagg     3300

cccagcgaca uuuauggagg cuacuccaac gagaaugggu cauuucuaac ccuggugaaa     3360

cuuaagaagg gaaguaaaua ugacuaccgg cucguggccg ucccuguuuc uaagaucagc     3420

gagaucaaag aauccgagcu ggcaacuauu gaaaaggcua ucgccccaca guucuugaaa     3480

ucaagcggua aguaucgaga uuuucaggug gugcugucua agauuuuaau aaaccaaaaa     3540

guaaucgacg auggcaugau uuucacaauc ggguccaaga guuacaaaca uaaugcaaag     3600

caggucgugc ucagcgacaa cucuauucag aucguuucca cccugucaag aauaagugau     3660

aaagagaaac uuaucacugu guuugacgaa auugucgaua ucaccagcaa guaccugccc     3720

ugguauaagg ugaaugacgu ugagaagaca aaaauguugu uccgccaaau gcaggauauu     3780

gacgagauga agaaaaucau agauaacucg cucauuggac ugcacugcaa ugccuccauc     3840

acggaccuag gcaagguggg ucugucuaaa uacuggggga gguucguacu uaccgcuggc     3900

aucagccuca cugucgaugc gcagcugauu uaucagucac cgacaggauu guuugaacau     3960

cgugugaagg ugagugacuu auga                                            3984


<210>  2940
<211>  3990
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2940
augaacaagc agcccuacaa uaucggccug gacauuggga ccagcuccgu gggauggucu       60

gccacagagc ucgauaacag acuucugcac auaaaagguc auaauggcau cggggucagg      120

uuguucaagg aaggagaguc agcugcagac cggcgcggcu uucgaacuac gcguagaagg      180

uuagcccggc gcaaauggag acugaggcua cucaacgaaa uuuucgcgac cgagaucgcu      240

aagguugauc cuagcuuuuu cgcccggcug aaacaaagua augugucccc aaaggacccg      300

aacaagacaa uguuugguaa uauucuuuuc gaugacgaaa accuggauga ccagaaauuu      360

caccaugauu auaagacuau cuaccacuug cgccaggcaa uaaucaauca ucccgagcag      420

aaauucgaca uucgacucau cuaucuggcc augcaccaca uuaucaagua cagagggcau      480

uuucugaacc aagcuaaugu acaggauuuc aaaggaggca agauagaccu cgagaagucu      540

uuuaaagcau uaaacgauau uuucgaaaau cagggcaggg accugcggcu ugugaccgac      600

gaggccaacc aguaugucaa gguguugucg gauaaugcua aaacacguuc agaccgcaag      660

aaagaacuga gcaagcuacu cuacguuccu aucgauaaag acaccgauaa gaacaguaag      720

aaagugacug gggagauucu gaaggccauc cuuggaaaca aagcgaaguu ugacgucauc      780

uucgguauug agguggaaaa uccaaaagag uggguacuga cauucaacuc cgaugacuuu      840

gaugacaaga uagaaggcuu gucuagccaa augaccgaug aggacuccga aauccuccug      900

auuuuaaagg agcuguauuu cgcacucaau cuuucagaca uccugaacaa uaguguuacg      960

gauaaaaacg ccaagacuuu gagcgaggcu augauaaaua gauacgacga ucacaaaaac     1020

cagcuagcca ugcugaagaa agugauugac ucuuccgaua agaagcaugc aaaagaacuc     1080

aaggaggcuu acucagccua uaucgacggg gaaaauaaca aaaagaucag cuuugaggau     1140

cugaaaaaga ggauucagaa gaaucuuguc gacagugaac ugucucagaa aaucaacgau     1200

uugcucgacc acgauuacuu ccugcccaag caacggucca aagagaaugg agcgauuccu     1260

caccaguuac agcaacagga gcuggaccag auaaucgaaa gccagaagca auauuaccca     1320

uuucuagccg agcucaaccc caaugaaaaa cgaucgcgcc aggcaaagua uaaacuggau     1380

gagcuugugg cuuucagagu gucuuacuau gucggcccua ugguugaagu gaccgacucc     1440

aaccaggaga acaaugguaa guuugccugg augauuagga aggagcaggg ggaaaucaca     1500

ccauggaacu ucgacgagaa aguagaucgg acugaaaccg cuacaaaguu uaucacccgc     1560

augacgacua cagacaccua cuugauugga gagccggugc ugcccaaaaa uucacuccug     1620

uaccaaaagu ucgucgugcu uaacgagcug aauaacuuga aaauaaauca ggaaacuauc     1680

acaccuggcc agaagcagua ucucuacgag cauguuauga agaaaaacaa gagagugacc     1740

cugaaaaaga uugccgaugc auuagucauc aaaggcgacu auccauacgc ccccgaagug     1800

aaggggacga ccgaucaaaa gaaucugaac aauggacuaa caacuuauau ugaccucgcu     1860

aaaaucuuug guaacgagcu ggaugacgua gauagggaag cggacuucga gaagauaauc     1920

gaguggagua ccauuuuuga agaucagcac aucuucguuc uuaaauugca ggagcugaag     1980

uggcucacag aaaaacagcg uaaucaagug gucaagcugc gguaccaggg cugggggaag     2040

uuaagcaaag agcugcuugc aggauuggug gacgauaacg gccagcgaau uaucgaccuc     2100

cugugguccg acaaucaaaa cuucaugcag auagucaacc agaagucuuu uaaagaaagc     2160

auugccgauc auaauggugc ucaccuagag gagaagaacc ugaaagacgu gaucaaugau     2220

cucuauacuu caccucagaa caagaaagcc auuagacaag uuauccuggu gguagacgau     2280

auaaucaaug cagucgggua cgaaccagcc aacauuauga uggaguucgc uagggaagac     2340

aguaaugauc aucgccuuac caacucccgg agcagacagu uggagaaugu guacaaggac     2400

aucacaaacu cuugguuuga uaaugacuca guuaaggaug aacuggagga caaaauuaag     2460

aacaaagaca aguucaccga uagguuauau cucuacuuua cucagggcgg aaaagaccug     2520

uauacgggcg agccccuuaa uaucgaugac cugaguagcu acgauauaga ccacauuuug     2580

ccgcaguccu ucauccucga uaacucucug gacaaucgcg ugcuaacauc gcaacgaaac     2640

aaccggguca gcaaggaaga ucagcugccu ggggagcucu uuggaaccaa gaugcacgcc     2700

uucuggaaac gucugaaggc agcgcaucuu aucuccaaua aaaaguauua caacuugacu     2760

cugaccccca auucaauuag uaaauuuaac aaucagggua gauucaucaa caggcaguua     2820

guggaaacac gccaaguaau uaagcucgcu gccgagauac ugcacaaucg guauucugag     2880

gaaaagggca ccaacaucgu gacuauuaaa gcaaaucuua cacaucacau gagagagaag     2940

uuuaacuucu acaaaaauag gaacguuaau gacuaccacc augccuuuga ugcuuaucug     3000

acggccuucg ucgggaacug gcuacuccag caguacccaa agcugaaacc uuauuucgug     3060

uacggagacu uugcaaagac cgaaaucaau aaccuaaaga gcuucaauau gcuguauaaa     3120

uuugagcaag cucaccaguu aaacgaaaag gauggcaaaa uugccaagga cacugacgcg     3180

cuccuggguu acaugaaaaa cguguaucau uucaagaaga ugcuugucac aaaagagcug     3240

gagaccaauc acggggccuu guuuaaccag acacucuacc ccuccccagc ucaugauauc     3300

aagaaacgac agcugauaaa gcccaaagac aaucgcccua cugauaucua cggcggauau     3360

acccacgagg aacggaagua caugucauug guuaaaguga uuggcaagaa ggacaacgaa     3420

uauagaaugg caaaugugcc acugcuuaaa aucaacaaga auaacgauau uaaagagauc     3480

cucaagagcc aauucucuaa uaaaaaguuu gaaauaguac ugccgaaggu cuugaaaaac     3540

cagcuguucu ccgagaaugg ggccuuauuu gugauuggaa guaaggguua caaauauaac     3600

gcuaaucagc ucguucugag cgaccuugau augaagcugu ugaacgaccu cucuaauaac     3660

aaauccaucg augaggugac gaucaaccag cuguucaaga auauugucga cucaaccaac     3720

aaguacaugc cccuauauga aaucaaaaau cugaacgagg ugguugaaaa guuuaauaac     3780

cuugaguuca augauaaaaa gaaaacaaua acugacuucc uccuggccac ccacgcaaac     3840

ccuacaauga guaauuugaa gaacauuggc uuaccauacu uugggagguu cauccguaau     3900

acuggaauua agcugagcga aaacgcgaau gugaucuacc aaucgcccac cggccucuuu     3960

ucccgcaaaa uaaagaucuc ugauuucuga                                      3990


<210>  2941
<211>  3993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2941
augaagaacg acuacaccau cggccuggau auugggacaa auagcguggg auauuccguc       60

guuacugacg auuacaaagu gauaucuaag aaaaugaacg uauucgguaa uacggagaag      120

aaaucaauca agaagaacuu uuggggcgug agacucuucg aaagugggca gaccgcccaa      180

gaggcuagga ugaaacggac aagccgccga cguauugcaa gaaggaagaa ucggaucugc      240

uaucuucagg aaauuuuuca gcccgagaug aaccaccugg acaauaacuu cuuuuaccgc      300

uugaaugaau ccuucuuagu cgccgaugac gcgaaauaug auaagcaucc uaucuuugga      360

acucuggacg aggagauaca cuuccaugaa caguuuccaa ccaucuacca ccuaagaaaa      420

uaucucgcug auggcgacga gaaggccgau cugaggcuug uguaccuggc aauugcccau      480

aucauuaagu uccgggguaa cuuuuugauc gaaggggagc ucaauacaga gaacaauucu      540

guuauagaac ugucgaaagu guucguccaa cuguacaacc agacccucuc agaguuagaa      600

ggauuucagu ucauugacga gagcaucgau uucagugaag ugcugacuca gcaacuuucc      660

aagucugagc gcgcugacaa uguauugaaa cuguuuccgg acgagaaggg cacaggcauu      720

uucgcacagu uuaucaaacu aaucguuggg aaccagggaa acuucaagaa aguguuucag      780

cucgaagagg aucaaaagcu gcagcuuagc accgacgauu augaagagaa uauugaaaac      840

cuguuggcca uaaucgguga cgaguacggc gauauuuucg ucgcugccca gaaucucuau      900

caagcgaucc uguuagcagg gauacugacg uccacugaga agacccgagc caaacucuca      960

gcuaguauga uucagagaua cgaagaacac gccaaggacc uuaaacuguu gaagagguuu     1020

gugaaagaac acauccccga uaaguaugca gagaucuuca acgacgcuac aaagaaugga     1080

uacgccggcu auauugaugg uaaaacuaag gaagaggagu uuuacaaaua ccuaaagacc     1140

acacuggugc agaaaagcgg guaucaguac uucaucgaaa agauugagca agaaaacuuu     1200

cuccggaagc agcguauaua ugacaaugga gucaucccuc aucagguuca cgcggaggag     1260

cugcgcgcca uucuuagaaa acaggaaaag uacuauucuu uccugaaaga gaaccaugaa     1320

aagaucgagc aaaucuuuaa agugaggauu ccauacuaug uaggccccuu ggcaaagcac     1380

aaugaacagu cccgguucgc uuggaacaua cgaaagucag augagccuau ccgcccaugg     1440

aauaugaacg acguggucga cgagaaugcc agcgcugugg ccuucauuga aagaaugacc     1500

aucaaagaua uuuaccucaa cgagaauguu cugcccaggc acaguuuaau cuacgaaaag     1560

uuuacggugu ucaacgagcu gacuaaaguc cuauaugcag acgaucgggg cguguuucag     1620

cgcuucucug ccgaagagaa ggaggacaua uuugaaaaac ucuucaaguc cgagagaaaa     1680

guaacaaaga agaaacugga aaacuaccuu aggaucgagu ugagcauuuc gucuccuucc     1740

guuaagggga ucgaggagca guuuaaugcu aacuucggaa ccuaucugga ucucaaaaag     1800

uuugacgagc ugcauccaua ccuugaugac gaaaaauauc aagauacucu ggaggaagug     1860

auuaaggucu ugacaguguu cgaggaccgu ucaaugaucc agaaucagcu cgagcagcug     1920

ccguuaaacc ugaguaccaa gacgauaaaa gcgcuaagcc ggcgaaagua caccgguugg     1980

ggcagacucu ccgcaaggcu gauugauggg auccacgaca aaaauucugg aaagacaauu     2040

cuugauuauu ugaucgaaga cgagagcgac ucauacauag ucaaccgcaa uuuuaugcaa     2100

cugaucaacg augaccaucu caguuucaaa aagauuaucg aagauuccca gcccuacaag     2160

gagcagcaaa gcgccgaaga gauugugucu gagcugucag gcaguccugc uaucaaaaag     2220

gguauauuac agagccugaa aauuguugac gaacuugugg ccaucauggg guauaagccc     2280

aaaaauaucg uagucgagau ggcacgggaa aaccagacua ccggcagagg aaagcaaaau     2340

uccaagccaa gguugaaagg cauugagaac gggcucaagg aauuuucuga uucggugcug     2400

aaaggaagcu ccaucgacaa uaagcagcua cagaacgauc gccuguaccu cuauuaccug     2460

cagaauggua aagacaugua uacaggccac gagcuugaua uugaccauuu gucaacuuac     2520

gauauagacc acaucauucc ucagaguuuc cugaccgaua acucuaucga caaccgaguu     2580

uuaacaacca gcaaguccaa ucgggggaaa ucagacaacg ugccaagcga ggaagucgug     2640

cguaagaugg auagauucug gaggaagcuc cugaaugcca aacuuauuuc ugagcgcaag     2700

uauacuaacc ugacgaaaaa ggaauugaca gaguccgaca aagcuggauu ucucaagcgg     2760

caacugguag aaaccagaca gaucacuaag cacguggcca ccauacuaga uaguaaauuc     2820

aaugaggaca gcaacaauag ggauguucag aucauuacac ugaagucugc acucgucucc     2880

gaguuucgaa aaaccuucaa ccuguacaag gugcgcgaaa ucaaugaccu ucaucacgcg     2940

caugaugcuu acuugaacgc cguggucgca cugucauuac uccggguuua uccccagcug     3000

aaaccggagu uuguguacgg cgaauauggu aagaauagua uucacgacca aaacaaggcc     3060

acuaucaaaa agcaguucua cagcaauaua acaagauauu uugcuucgaa agauuacauu     3120

aucaacgacg auggggagau ccuuuggaau aagcaggaga cgauugccca agugauaaaa     3180

acccugggca ugcaccaggu aaacgucgug aagaagguug aaauccagaa aggaggcuuc     3240

uccaaggagu cuauucagcc uaaaggggaa agccaaaagc uaaucaggcg uaaacagcag     3300

uggaacacua agaaguaugg agguuuugac ucacccgugg ucgcauacgc uauucuccug     3360

aguuucgaca aaggcaagcg caaagcccgg uccuuuaaga ucguggggau aacaauccag     3420

gauagagagu cuuucgaagg aaauccaauu uuguaccuga gcaaaaagga cuaucauaac     3480

ccuaaaguug aggcgaucuu acccaaguac ucccucuucg aguuugaaaa uggcaggcga     3540

cggauggugg ccucagcuag cgagacccaa aaggguaacc agcugauuau cccagggcac     3600

cuuauggaac uguuguauca uaguaaaaag auaauuaaug gcaaaaacuc ugauuccgua     3660

agcuacaucc agaauaacaa ggagaaauuc cgcgaaaucu uugaguauau ugucgacuuc     3720

ucgucaaagu acaucucugc agaugccaau cucaacaaga uagagaaaau uuuugaaaau     3780

aacuuccaca aggcuaguga gcaggaaauc gccaaauccu uuauuaaucu gcuaacauuc     3840

acugcaaugg gagcgcccgc cgacuuugag uucuuuggcg aaaagauccc uagaaaaagg     3900

uaugugagca uaucugagau uaucgaugcu guguucaucc aucaaagcau uaccgggcug     3960

uacgagacgc ggguccgacu uaccgaaguu uga                                  3993


<210>  2942
<211>  3993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2942
auggagaagg aauacaccau cggccuggac auugggacaa acagcguggg augggccguc       60

cucacugaug acuauagacu uguugcuagg aaaaugucca uacaggguga uucuaaucgg      120

aagaaaauca agaaaaacuu cuggggcgca cgccuguuug aggaagggaa gacggcccaa      180

uuccgacgua uuaagagaac caauaggcgg cgcaucgcga gaaggcggca gcgcguguug      240

gcuuuacagg acauuuuugc cgaggaaauc cacaaaaagg aucccaacuu cuuugcacga      300

cuggaggagg gagacagagu agaagccgau aaaagguucg cuaaguuucc uguguucgca      360

acacuaucag aggaaaagaa uuaccaucgg caguauccaa cuauauacca ccuccgucau      420

gaccuggcca acaguaagga gcaagcugau auccgccuug ucuaucuggc cauugcgcac      480

ugcuugaagu acagaggcca uuuucucuuc gagggugaac uggacaccga gaauacaagc      540

gugaccgaaa acuaucagca guuucugcag gcauaccaac aguucuuucc ggagcccauc      600

ggggaucucg acgaugccgu uccuauuuua acugaaaggc uguccaaagc uaagcgggug      660

gagaaagucc uugccuacua uccaucugag aagucgacag gaaauuucgc acaguucuug      720

aaacugaugg ugggcaacca ggcuaauuuu aagaaaaccu ucgaccuaga agaggaaaug      780

aagcucaacu uuacgcgaga cuguuacgag gaagaucuga augagcuucu ggagaagacu      840

ucagacgauu augccgaauu guuccucaaa gcgaagggcg uauacgacgc cauccuguua      900

agccaaauac ugaguaaauc cgaugacgag accaaggcaa aacucucugc uaacaugaaa      960

cuucgcuuug aggaacacca gagagaucug aagcaguuga aagagcuagu uaggcgggac     1020

cugcccaaga aauaugauga cuucuuuaag aaucgcagca aaaacgggua cgccggauau     1080

gugaagggua aggcuacaca agaagauuuc uacaaauuuc ucagaacuga gcuggccggc     1140

cuugaagagu cccagucaau uauggaaaag aucgaccugg agauuuacuu gcucaaacag     1200

aggaccuucg caaacggggu caucccucac cagauccauc ugguggagau gcgugaaauu     1260

auggaccggc aaaagcgauu uuauccauuc uuaaaaggag cccagggcaa gauagagaag     1320

cugcuaacau uuagaauccc cuacuaugug gguccucucg cucaggaagg gcagagucca     1380

uucgcgugga uuaaacgcaa gagcccgucu caaaucaccc ccuggaauuu cgcagagguc     1440

guugauaaag aaaacuccgc cauagaguuu auugagagga ugacgaauca ggacacuuac     1500

cugccuaagg aaaaagugcu uccaaagcag ucauugaucu aucagcgguu caugaucuuu     1560

aacgagcuga caaaaguaag cuacaccgau gaaagaggaa agagucacua uuucucuucc     1620

gagcaaaaga ggaaaauuuu uaaugaacuc uucaagcagc auccccgcgu gacugagaaa     1680

cagcugcgaa aguuucuuga gcugaacgaa cagaucgaca gcacagagau uaaaggcaua     1740

gaaaccucgu ucaaugcuuc uuacuccacg uaccacgauu ugcucaagcu gucagaccaa     1800

auggauaccu uacuggacga uccugacaug acaacuaugu uugaggaaau cauuaagauc     1860

cuaaccaucu ucgaggaucg ggagaugauu cgugaacagc ucaaaccaua ugagacaguc     1920

cugggccuuc ccgccauaaa gaaauuggca aagaaacacu acacugggug gggaagacug     1980

agugaaaaga ugauccaggg uaugagggag aagcaaagcc gcaaaaccau ucucgacuau     2040

cugaucgaug acgacgauuu uccgugcaac cggaauagaa acuucaugca guuaauuaau     2100

gacgaucauc uguccuuuaa ggagacaauc gccaacgaac uuauaauguc ugacagcaau     2160

guguugcucg aucagguuaa agcuaucccu ggcucacccg ccgugaagaa agggauuugg     2220

cagaguauca agauugucga ggaaaucaua ggaauuaucg gcaaggcacc caaaaacauu     2280

gugaucgaga uggcgaggga aaaucaacga accucccgca gccggccuag acugaaggcu     2340

cuagaggagg cccugaaaaa cauagacucu ccccuccuga aggauuaccc aacugacaac     2400

caggcacuuc agaaagauag guuguaucug uacuauuuac agaaugguaa ggacauguac     2460

acgggggaac cccucgagau ccaccgucug ucagaauacg auauugacca uaucauuccu     2520

cgcaguuuca ucguagauaa cagccuugac aauaaaguuc ugguguccuc uaaggucaac     2580

cggggcaagu uggacaaugc cccagauccg cucgugguca aaagaaugag gucgcacugg     2640

gagaagcugc aucaagcuaa acuaauaagc gacaagaaac uggccaaccu cacaaagcag     2700

aaucugaccg aagcagauaa ggcucgauuc auucagcggc agcuugugga gacucgccaa     2760

aucaccaaac acguugccaa cuugcugcac cagcauuuua auuuacccga ggaagugucc     2820

gcgacagaga agaccucaau cauuacucuc aaaaguacac ugacgucuca guucagacaa     2880

auguuugaca ucuauaaggu aagggaaauu aaccaccauc accacgccca ugaugcuuac     2940

cuuaauggag ucguggcaau gacccugcuc aaaaaguauc cuaagcuagc cccagaguuc     3000

guuuacggca gcuauauaaa aggggacauc aaccagauua auaaggcuac ugccaaaaag     3060

gaauuuuacu ccaacaucau gaaauucuuu gagucagagg aaauuaucug ugaugagcag     3120

ggagaaguga uauggaauaa gaagcgggac cugagcacaa ucaaaaagac cauuggugca     3180

caccagguca acaucgugaa aaagguagag aaacaaaagg gcggguucua uaaggagaca     3240

auuaacucua aagcgaaucc cgaaaaguug aucccucgaa aagccucccu ggauccauua     3300

aaguacggag gcuacgguag uccccucgug gcuuauacug uuauauuuau uuucgagaaa     3360

gggaagcaga aaaaggucac caagggcauc gaaggaauca cggugaugga gcagcugaga     3420

uuugaacagg acccgcgcga guuccuuaaa accaagggcu acgagggggu gaaacaaugg     3480

cugauuuugc cuaaguauau acucuucgaa gcccagggag guuacaggcg uaugaucgca     3540

agccaucagg agacacagaa agcuaacucu cugauucuac cagaaaaucu ggucacucuu     3600

cucuaucacg cccggcauua cgaugagauc aaccacaagg uuuccuuuga cuaugugaau     3660

gcacacaagg aaggcuucaa cgacauuuuu gauuucaucu cagacuuugg ggugagauac     3720

auacuggccc cacaacauuu ggagaaaauc aagguagcuu acgagaaaaa uaaggaaguc     3780

gaucugaaag agaugauuga cgcgaucuua agucuccuga aguucacccu uuuuggagcc     3840

agcguggagu ucaaguuuuu cgauauuaaa auccugaaga gguauaaauc guugacagac     3900

auaugggaag caacuauuau cuaccagucc guuaccggcc ucuaugaacg ccgaguggag     3960

gucagaaagc ugugggaugg ugagcggcua uga                                  3993


<210>  2943
<211>  3996
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2943
augaacaagc agcccuacaa uaucggccug gacauuggga ccagcuccgu gggauggucu       60

gccacagagc ucgauaacag acuucugcac auaaaagguc auaauggcau cggggucagg      120

uuguucaagg aaggagaguc agcugcagac cggcgcggcu uucgaacuac gcguagaagg      180

uuagcccggc gcaaauggag acugaggcua cucaacgaaa uuuucgcgac cgagaucgcu      240

aagguugauc cuaguuucuu cgcccggcug aaacaaagca augugucccc aaaggacccg      300

aacaagacaa uguuugaaaa uauucuuuuc gaugacgaga accuggauga ccagaaauuu      360

caccaugauu auaagacuau cuaccacuug cgccaggcaa uaaucaauca ucccgagcag      420

aaauucgaca uucgacucau cuaucuggcc augcaccaca uuaucaagua cagaggucau      480

uuucugaacc aagcuaaugu acaggauuuc aaagggggaa agauagaccu cgaaaagucu      540

uuuaaagcau uaaacgauau uuucgagaau cagggcaggg accugcggcu ugugaccgac      600

aaggccaacc aguaugucaa aguguugucg gauaaugcua agacacguuc agaccgcaaa      660

aaggaacuga gcaaacuacu cuacguuccu aucgauaagg acaccgauaa gaacaguaaa      720

aaggugacug gcgagauucu gaaagccauc cuugggaaca aggcgaaauu ugacgucauc      780

uucggaauug agguggaaaa uccaaaggag uggguacuga cauucaacuc cgaugacuuu      840

gaugacaaga uagaagguuu gucuagccaa augaccgaug aggacuccga aauccuccug      900

auuuuaaaag agcuguauuu cgcacucaau cuuucagaca uccugaacaa uaguguuacg      960

gauaagaacg ccaaaacuuu gagcgaggcu augauaaaua gauacgacga ucacaagaac     1020

cagcuagcca ugcugaaaaa ggugauugac ucuuccgaua agaaacaugc aaaggaacuc     1080

aaagaggcuu acucagccua uaucgacggc gaaaauaaca agaaaaucag cuuugaggau     1140

cugaagaaga ggauucagaa aaaucuuguc gacagugaac ugucucagaa gaucaacgau     1200

uugcucgacc acgauuacuu ccugcccaaa caacggucca aggagaaugg ggcgauuccu     1260

caccaguuac agcaacagga gcuggaccag auaaucgaaa gccagaaaca auauuaccca     1320

uuucuagccg agcucaaccc caaugaaaag cgaucgcgcc aggcaaaaua uaagcuggau     1380

gagcuugugg cuuucagagu gccuuacuau gucggaccaa ugguugaagu gaccgacucu     1440

aaccaggaga acaauggcaa guuugccugg augauuagga aagagcaggg ugaaaucaca     1500

ccguggaacu ucgacgagaa gguagaucgg acugaaaccg cuacaaaauu uaucacccgc     1560

augacgacua cagacaccua cuugauuggg gagcccgugc ugccuaaaaa uucccuccug     1620

uaccaaaaau ucgucgugcu uaacgagcug aauaacuuga agauaaauca ggaaaucauu     1680

acuccaggac agaagcagua ucucuacgag cauguuauga aaaagaacaa aagagugaca     1740

cugaagaaaa ucgccgaugc auuagucauu aagggcgacu aucccuacgc cccugaagug     1800

aagggcacca cggaucagaa aaaucugaac aaugggcuaa ccacauauau cgaccucgcu     1860

aagauauuug gaaacgagcu ggaugacgua gauagggaag cggacuucga gaaaaucauu     1920

gaguggucaa cuaucuuuga agaucagcac auuuucgcac uuaaguugca ggagcugaaa     1980

uggcucaccg aaaagcagcg uaaucaaguu gugaagcugc gguaccaggg uuggggcaaa     2040

uuaaguaagg agcugcuugc cggguugguc gacgauaacg gacagcgaau cauagaccuc     2100

cuguggagcg acaaucaaaa cuucaugcag auugugaacc agaaauccuu uaaggaaucu     2160

aucgcugauc auaauggcgc ccaccuagag gagaaaaacc ugaaggacgu cauuaaugau     2220

cucuauacaa gcccacagaa caaaaaggca aucagacaag ugauacuggu uguggacgau     2280

aucauuaaug ccguagguua cgaacccgcu aacaucauga uggaguucgc cagggaagac     2340

ucaaaugauc aucgccuuac uaacagucgg uccagacagu uggagaaugu cuacaaggac     2400

auuaccaaca gcugguuuga uaaugacucu gugaaagaug aacuggagga caagaucaaa     2460

aacaaggaca aauucacaga uagguuauau cucuacuuua cccaaggggg caaggaccug     2520

uauacuggag agccgcuuaa uauagaugac cugucaaguu acgauauuga ccacaucuug     2580

ccucagagcu ucauccucga uaacucccug gacaaucgcg uucuaacguc ucaacgaaac     2640

aaccgggugu cgaaggaaga ucagcugccc ggcgagcucu uugggacaaa aaugcacgca     2700

uucuggaagc gucugaaagc ggcucaucuu auuagcaaua agaaauauua caacuugacc     2760

cugacuccaa auuccaucuc aaaguuuaac aaucagggaa gauucauuaa caggcaguua     2820

gucgaaaccc gccaagugau aaagcucgcc gcagagaucc ugcacaaucg guauagugag     2880

gaaaaaggua caaacauugu aaccaucaag gccaaucuua cucaucacau gagagagaaa     2940

uuuaacuucu acaagaauag gaacgugaau gacuaccacc augcuuuuga ugccuaucug     3000

acagcauucg uuggcaacug gcuacuccag caguacccua aacugaagcc cuauuucguc     3060

uacggggacu uugcuaagac ggaaauuaau aacuugaaau cuuucaauau gcuguauaag     3120

uuugagcaag cccaccaguu aaacgaaaaa gauggaaaga ucgcgaaaga cgccgacgcu     3180

cuccugggcu acaugaagaa cguguaucau uucaagaaaa ugcuugugac caagaaacug     3240

gagacuaauc acggugcauu guuuaagcag acacucuacc caagccccgc ccaugauaua     3300

aagaagcgac agcugaucaa accuaaggac aaccgcccaa ccgauauuua cgggggcuau     3360

acacacgagg aacggaagua caugucccua gucaaaguua ucggaaagaa agacaaugag     3420

uauagaaugg cuaacgugcc gcugcuuaag auuaauaaaa acaaugauau caaggaaaua     3480

cucaagucac aauucagcaa caaaaaguuu gagauugugc ugcccaaagu auugaagaau     3540

cagcuguucu cugaaaacgg cgccuuauuu gucaucgggu ccaaaggaua caaguauaau     3600

gcaaaccagc ucgugcugag ugaccuugau augaagcugu ugaaugaccu cagcaacaau     3660

aaaucuaucg augagguuac uauuaaccag cuguucaaga acauagugga cuccaccaau     3720

aaauacaugc cucuauauga gaucaagaac cugaaugaag ucguggagaa auuuaacaau     3780

cuugaauuua acgauaagaa gaaaacgauu accgacuucc uccuggcgac acacgccaau     3840

ccaacuaugu caaacuugaa gaauaucggu uuacccaguu acuuuggcag guucaccagc     3900

gggaacgcug gaauuaaacu gucggagaau gccaacguua ucuaccaauc cccuacaggc     3960

cucuuuucuc guaaggugaa aauaagcgau uucuga                               3996


<210>  2944
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2944
augaagaaca gagagaauau caaauacuau auuggcgugg acauagggac caacagcguc       60

ggaugggccg uuaucgauga aaaugguaac cugcucaaga aaggcaagca ccaucuuugg      120

ggguccaggc uguucgacca ggcucaaaca gcacagaauc ggcgcaacua ccgaucuuca      180

cguagaaggu auaauaaacg gcgccagaga auuggauugu uaaggcugau caugagugau      240

auggugcuag agguagaccc cagcuuuuuc auucggcucg aaaagacuac guuucuggau      300

aaggaggaca aaaaggccau ccuuaaagau aacuacaaga ugaauuauaa ccuguucugc      360

gacgaagauu acaaugacaa aaaguauuuu aaggauuacc cuaccauaua ccacuugcgc      420

aaaaagcucu gugaguccga cgagaaagcg gauccacgac ugaucuaucu ggcucuccau      480

cacauuguga aguacagagg caacuucuua uaugaagguc aggagcugca ucuugaaccg      540

ucuaauaaag aggaggacuu gaagauccug uuugacauuc uagggaaaaa caaugauaca      600

gucuacgaca ucucggaaga acaaauacag uucauucuca agacuguggu ugaaaacauc      660

ucaaagaccg ccaaagugga ugagugcaug agccagcuga aacuuaacag ugaagacaaa      720

aagauuguca aagaguuuau gaggggacug gugggcaaua aguucaacgu auccaaguug      780

uauaugcacg aggaucucca guuugacgau gaagaccuga aauuacaauu cucugauaag      840

agcuacgagg aaaagaucac agaguaugaa aauguucugg aggagaagau ggaauuuauc      900

gaccucaugc agcgguucua cuccuggauu gagcuuucaa aaauaguggg cagugauagc      960

cagcacgcau cuaucuccgg ggccaugguc aacauuuacg aaucacaucg ugaggaccug     1020

cgcaccuuga aggaagugau gcuaaagauc gguaaaaagg aguaugacga gauguucaaa     1080

cccacuagca agaauguggu caacuacuau aauuacguua acccuguggc uuguagugga     1140

gauaaaacag acggcuuuua uaaguacgua aagaaagcaa uagaaaaguc ugaugacucc     1200

agaaaagaug agauucugca gaagaucgcc aaugaaaccu auaugcucaa acaaacgagc     1260

aagaacaaug cuuacauccc auaccagaug cagaaagacg agcugauuaa gauccuugau     1320

caccaagaga aguauuaccc cgugcugaaa gaaaacaggg acaagauuau aucgaucuug     1380

gaguuccgga uuccuuauua cuaugggcca cucgauggaa auaaacaguu uggcuggcug     1440

aucaagaaaa aggguaagga aaacgagcga aucuuacccu ggaaucauca ggaaauuguc     1500

gacgugcagg agacugccgc gcaauucauc aaaaagcuga ccaacuacug cacauaucua     1560

ccuauagaga aaguuaugcc acagaagucu cucacuuguu ccauguacga agugcuguca     1620

gaggucaaca aaauucgcau cgaugggaaa cuuuugccga uugacaccaa gaauagacug     1680

aucgaagauc ucuuuuucaa aaggaagaca gugaaagaga aggaccugau aaacuggcuu     1740

aaacagaauc agcugaccgu aggagaaauc acgggcuacc aaaaggagaa ggcauuuagu     1800

agcuccuugg cccccuggau ugacuucaaa gagaucuuug augaaauuaa cgacucuaau     1860

uaugaucuca ucgagaagau aauugaagac augacuaucu ucaacgaggg cagcauucug     1920

aaagaacggu uaaagaaggc uuacaaucug gaucagaaca agguuaaaaa gaucaugaag     1980

cuaaaauauu caggguggag ucgccucucc aagaaacuga uaaauggaau cagagccgac     2040

aacaaguuug guagcucugu gucaauucuu gaugucauga aagagaguca cauggcauug     2100

auggagauca uuaaugacca ggaucugggc uucaagcaga ucauagaaaa ggagaacuuu     2160

aaaaaugaaa cagggagcuu cgaguacgag gacauugaaa accucgcugg auccccugcc     2220

cugaagaggg gcaucuggca aaccuuacag gugaucgagg aaauuaaaaa uuauaugggu     2280

cacgagccaa agaacaucua cauugaguuc gcgcgugagg agcaggaaaa aguccggacu     2340

acaacccgag ugaagaagcu gaaaucuaua uauaacgaua ucaagaauca gcuugacguu     2400

caugggaaag agguguacuc gaacuugaau aagcaagaug agaaaagcuc cauugaaaag     2460

agacucuacc uguauuacac gcagcuaggc aagugcaugu auucaggaga aagucuggac     2520

aucgacaaac ucucuaacua cgagauugau cacaucuuuc ccaggacccu gacaagcgac     2580

gauucccuug acaauuuggu acuggucaag aaaaaggaga accagcgcaa auuagaugac     2640

cucgugcugc cucuugaaau acggaauaag auggagguuu ucuggaagaa acuguaugau     2700

aacggcuuga ucacucaaac caaguacuau agacucauga gggacgaauu ucgccgagau     2760

cagauugaca aauucaucaa ucggcagcug guggagacac gucagauuau caagcauguc     2820

gccaacauaa uugaaaauca cuacgaggau acuaaagugu uuaccguaag agcaaaccua     2880

ucacaugagu ucagggaaaa guacgggauc uauaaaaauc gcaacgugaa ugacuuucac     2940

cacgcucaug acgccuacau cgcuuguauu auaggacggu auauccaaau uagauuccca     3000

ggucuggagg ccaaguacgu uuauggccag uacaugcaga acgucaagaa aacaaagaac     3060

aauaucgaua aagaaaacca cggguuuauu aucaauagca ugaaguauga gugcauagac     3120

gaggauaccg gagaagugau cuggaacccc gagaggauuc ucgacuacau caaauguuuc     3180

aauuaccgag auguguauau uacuaagaag cuggacacga acaaucgcga acuuuuuaac     3240

gucacaaucu ugccgaauga uaaaaacucu gagaagggca aaaccaaggc aacuauaccu     3300

guuaauaaac ugcgguccaa cgugagaaag uacggugggu ucaguggcuu acagagcgac     3360

auuguggcca ucgaaggaaa gaaaggcaag aaaaucgaua ggcgucucau uaaucugccc     3420

auccuucugc gcuauguauc uauagaggac caaugcaagu acauuaugga gaacaauaaa     3480

uauuccgaug ucaagaucau uaagaaaauc aagaaaaacc agcuaauaga aauugacggg     3540

aucuguuacu aucucaaguc agcugaugag cuggacaccg cguaccaguu gauccugagu     3600

cgggacgaua augaaaugau uuacagaauc gacagggcaa uucagaacaa cgauuuuagc     3660

uauuucgaca auaaaaagga gaacauagau gaauuauacc gacucaucug ccauaaaaug     3720

auugagaauu auccaaagua caagaaaaag guguaugagu ucaucaaaga acgggaggaa     3780

gaguuuuucg aacuggacaa cucgcaaaag ugcaucguua uuuuugagau gauacgcgug     3840

augaaaagag gagagcagua caucaauauu ccuuucgaac ccuauaaccu uaagaaugau     3900

aggaaggguc ggcugaacug ucaguccauc uugcucgaca auacauacuu uuacgauguc     3960

ucuauuaccg gcaucuauag caaaaaguac aaacuguga                            3999


<210>  2945
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2945
augcagaaga acuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

augaaagaug acuauacucu cauaagaaag aggaugaaag uucuugguaa cacggauauc      120

aagaaaauua agaagaauuu cuggggcgug cggcuguuug acgaggggga aaccgcuaaa      180

gagacacgcu ugaagcgagg aacucguaga agguaccaac ggcgcagaaa cagguuaauc      240

uaucugcagg auauuuucca gcagcccaug cuagcaaucg acgaaaauuu cuuccaccgg      300

cucgaugacu ccuuuuucgu accugaugac aaaucuuacg aucgccaucc aauauuuggc      360

ucacuggagg aagagguggc cuaucacaac accuacccga caaucuauca ucuucgaaag      420

caccuggcgg acaaucccga gaaagcugau uugagacucg ucuacaccgc ccuggcacau      480

auugugaagu acagggguca cuuccugauc gaaggggagc ucaacacuga aaauacaagu      540

auuagcgaga ccuuugagca auucuuagac acguauuccg acaucuuuaa ggaacagcug      600

guuggagaua uaucuaaagu ggaggaaauu cuuucgucaa agcagagccg gagucguaaa      660

cacgagcaga ucauggccuu guucccuaac gaaaauaagc ugggcaacuu uggccgcuuc      720

augaugcuaa uugucgggaa uacuuccaac uucaaaccag uguuugaccu cgaugacgag      780

uacaagcuga aacuuucuga ugagaccuau gaagaggacc uggauacauu gcucggaaug      840

acugacgaug uauuccugga cguuuuuaug gcugcaaaga auguguacga ugccgucgaa      900

augagcgcua ucaucuccac cgacacaggu aacucaaagg ccguguuaag uaaccaaaug      960

auuaauuucu augacgagca uaaaguggau cuggcgcagc ucaagcaguu uuucaaaacc     1020

caccuucccg acaaguacua ugaaugcuuu agcgauccuu cuaaaaacgg cuacgcaggg     1080

uacauagacg gaaagacgaa ucaggaggau uucuauaagu acaucgagaa agucaugaag     1140

acuauuaaau ccgacaagaa agauuauuuu cuggacaaga ucgauagaga aguuuucuug     1200

aggaagcaac ggucauuuua caacagcgug auaccacauc agauucaccu acaggagaug     1260

caagccaucc uggaccgaca gagucaguau uaccccuucc ucgcugaaaa ucgcgauaaa     1320

aucgagucuc ugguaacauu uaggauuccu uauuacgugg gcccacuuac cgucuccgac     1380

cagagcgaau uugccuggau ggagagacaa ucggaugagc cgauccggcc cuggaacuuc     1440

gacgaaauug ugaauaagga gcgcucugca gaaaaauuua uagagagaau gacuaacaug     1500

gacacauacc uguuggagga aaagguucuc ccuaaaaggu cccuguuaua ucagaccuuc     1560

gagguguaca augaacugac gaagguccgu uauaccaacg agcaggguaa gacagaaaaa     1620

cuaaaucggc agcaaaaggc ugagaucauu gagacucucu uuaaacagaa gaaccgagug     1680

agagaaaaag auaucgccaa uuaccuggag caguaugggu acguagacgg aaccgauauc     1740

aagggcguug aagacaaauu caacgcguca cuuaguacau auaaugauuu ggccaagauu     1800

gacggcgcaa aggcuuaccu ggaugaccca gaguacgccg auguguggga agacauaauc     1860

aaaauucuca cuaucuuuga ggauaaggcu augaggaaaa agcagcugca aaccuauagc     1920

gacacacuuu cccccgagau ucugaaaaag uuggaacgca agcacuacac cgggugggga     1980

cgguucucua aaaagcucau caacggucug agagaugagg gcagcaacaa aacuauauua     2040

gacuaucuga agucagacga agggaguucc ggaccuacga auaggaacuu uaugcagcua     2100

auccgcgaua auacacucag cuucaaaaag aagauugagg acgcccagac caucgaagau     2160

acuacccaua uuuacgacac agucgcagag cugccaggcu cucccgccau caaaaagggu     2220

auacgacagg cucuuaaaau uguggaggaa aucauugaua ucauagggua ugagccggaa     2280

aacaucgucg uggagauggc gcgggaguca caaaccacua agaaaggcaa ggacuugagu     2340

aaggaacguc uggagaaacu cacagaagca auuaaggagu uugauggacc uagcgacguu     2400

aaagugaagg aucugaaaaa ugaaaacuua agaaaugaca ggcuguaccu uuauuacuug     2460

cagaacggcc gcgauaugua cacgaaugag ccccucgaca ucaacaaucu guccaaguau     2520

gacauugauc acaucauacc acagucuuuc accacugaca acucgauuga uaauaaaguc     2580

cuaguaagcc ggacaaagaa ccaagggaau aaguccgacg augugccuuc aaucaacauc     2640

guucauaaaa ugaagcccuu uuggagacag cugcacaaag ccggacucau uagugacagg     2700

aaguucaaaa accugaccaa ggcugagcau gguggccuua cagaagccga ucgagcacac     2760

uuuuugaauc gccagcuggu ggagacucgg cagaucacca agcacgucgc caacuuacuc     2820

gacucucaau acaauacggc ugaagaacag agaauuaaca uagugcugcu uaaaagcucc     2880

augaccucaa gguuccguaa ggaguucaaa cuguauaagg uacgcgagau caaugauuac     2940

caucacgggc augacgccua uuugaacgca gugguugcga caacuauuau gaaagucuac     3000

ccaaaucuca agccccaguu uguguaugga caguacaaga aaaccagcau guucaaggag     3060

gaaaaagcua cagcccggaa gcacuuuuac ucuaacauca cuaaauucuu uaagaaggag     3120

aaagugguca augaagagac cggcgaaauu cugugggaua cggagagaca ccuauccaca     3180

aucaagaggg uucugaguug gaaacaaaug aacauaguga agaaagugga gaagcagaag     3240

ggucagcucu ggaaagaaac caucuauccu aagggggaca gcucuaaacu gauuccagua     3300

aaggagggca uggauccgca gaaauacgga ggccuuuccc aagucucaga agcauucgcc     3360

gugguuauca cucaugagaa ggggaaaaag aagcaguuga aaagugaccu gauuagcauc     3420

cccauagugg accagaaggc uuaugagcaa cacccuacag ccuacuuaga agaggcagga     3480

uauaauaacc caaccguccu ccaugaacug uuuaaauacc agcuuuucga gcuggaagau     3540

gguucgcgac ggaugauugc uuccgccaag gaguuucaga aaggcaauca gauggugcua     3600

ccccucgagc ugguugaauu gcuguaucac gcgaaccgcu acgacaaggu gaaguucccu     3660

gauucuaucg aguacguaca ugacaauuua gccaaauuug augaccuccu ggaauauguc     3720

aucgauuuca gcaacaagua cauuaacgcu gacaaaaaug ugcaaaagau acagaaaauc     3780

uauaaggagc acgggacuga agauguggag cuuaccgucg agucauucgu uaaccugaug     3840

acauuuacgg caaugggagc cccagcuacc uucaaguuuu acggcgaaag uauuacuaga     3900

uccagguaua caucuaucac cgaguuccgg gguagcacau ugauuuuuca guccaucacu     3960

gggcucuacg aaacccgaua uaaacuggag gacaauuga                            3999


<210>  2946
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2946
augaugaagg aguacagcau cggccuggac auuggguccu cuucaguggg auucgucgcc       60

guugaugacc aguauagagu gguaagggcu aaagguaaga acgugauagg cacccggcuc      120

uuugaugaag ggaaaacagc agccgaccgc cgaggacuuc guacuacgag aaggcggcug      180

agucgcagaa aguggagguu gcgguuacug cgcggcaucu ucgagcccca cauuaaugaa      240

aucgaugaga gcuuuuucau gcgacuaaaa gaauccaaca uuucuccuaa ggacucgaau      300

aagcauuuuc caggugauau ccucuucaac gacagaucag auagggaguu uuacgagaau      360

uauccgacca uauaccaucu gcggcaagcg cuuaaaacag aaaagcguca guucgacauc      420

cgcgagauuu aucuggcuau gcaucacauc gucaaauaca gagggcacuu uuuggaagga      480

ggcacuguga ccgauuucga gguuggcgag cucgaccugg ccgggaaguu uagcaggcug      540

aacgaacucu ucgagaguau uaauuuagaa gugcccuuug uccugcggcu ugauaacuug      600

gaggacguga aagcaguacu gaucgacucc cagcgaucug cccaggaucg ccaaagaaca      660

cuaaccccug acauauaugu uccaagcacu gauaagauua cagaaaagag guucaaagga      720

aucgcuaccg agcuccugaa ggcaauugug gguaauaaag ccaaguucga cguccuugcu      780

ggccuggagu uggauaaaga agaggccaag cucugggcgc ugacguuuga cuccgaaacu      840

uucgaugagg acaucgaaca guuagagggg gauaugaccg aggcaacaca ggaccugauc      900

gaaauucuca aaucacuucg gagucugaua cgcuuggccg ugaacaugcc cgagggagug      960

agccuaucug aaaugaugau ccagagauac gaggaucacg cugaccaccu gaagcuccug     1020

agggacauua uccauaauac ugaugccgaa aaggcagcug gccuucaagc cgcguacgac     1080

gguuauauag auggggucaa aggaaagaaa accccuguug acgauuuuua caaggccauu     1140

aaaaagaacc uggacgauuc cgaggcagcu caccagaucu uggcccucau cgacaagaau     1200

gaguucaugc caaaacagcg uucaaagagc aacggcugga uuccccauca gauccaacag     1260

aaagaacugg aucggauuau agagaaucag aagcaauauu acccuugguu agcugacgug     1320

aacccaaacg aaagucgaag aaaaguagcc ccguauaaga ucgaugagcu ggugacauuu     1380

aggguccccu acuauguggg cccucuaguu accucugagg aacagaagaa auacgggucc     1440

aaguucgcau ggauggugcg caaagaggaa aagggagaga uuacgccaug gaauuuugac     1500

cagaaagucg aucgggccgc uagcgcgacu gaguucauca acagaaugac aaccauggac     1560

acuuaccucc ugaaugaaga cgugcuuccc gcacacucgu ugaucuauca gaaguacgag     1620

guacugaacg aacucaauaa gguuaaaauu aacggugaug ccauaacacc ugagcugaag     1680

cagaaaaucu aucacgaccu uuuugaaaag caaaccacgg ugucuauuaa aaaucugcag     1740

accuacuugg ucuccgaguc agaagcuagu gaggugccag agaucaaggg ccucgccgau     1800

gagaaaaagu uccugagcgg guuaacaacu uaucaugacc ugaugaagau uuuugauagg     1860

aacgucguga augaccccga acgcgcagaa gauuucgaga aaaucauacg augguccacc     1920

guuuuugaag acaagcacau cuaccgggag aaacuagauu cucucagcug gcugacaacu     1980

gaccagaagg aucgucuugc cgugaaaaga uauaggggau ggggccgcuu gucagagaag     2040

cugcucauug gucugcggga cgaagggcau agaaguauca uggauuacuu augggagacc     2100

acauccaacu ucaugcaaau ucaggcucag aaugacuuca gcgaaaagau cgccgcagag     2160

aacaaagagu uucuggacaa uacccagucu guacaaggca uaauuaacga acuuuacacu     2220

ucaccgcaga auaagaaagc gaucaggcag acguugcucg ucguggagga uauuaagaag     2280

gcuuuccacg gagaagcccc uaaacgaauc uuuguugagu ucgcacgcga aaacgagagu     2340

acaccccagc ggagcauaca aagacagcgu cagguggaga acgccuauga aaaggucacc     2400

caaaaucugc uaguggacga uaaaaucagg gcugagcuga aggacuccau uaacgaaaaa     2460

cgccucuuuc augaucggcu guuccuuuac uuuaugcagg gcgggaucga cauguauucu     2520

ggaaagaaaa uuaauaucga uaacuugucg gccuacgaca uagaucacau ucugccacag     2580

agcuucacug uagacaauuc cuuagauaac agagugcuca ccaaucagaa ggacaacagg     2640

gagaagggua auucacugcc uagugaacuu uaucgaggcu cuaugaaaga gacaugggag     2700

augaugcgga accaagggcu guugagcaag ggaaaauuuc accgccucau gaugaccccc     2760

aauuccauga cugcauacga ccagcagggc uucaucaaca gacagcuggu ugaaacaagg     2820

caagucauca agcuagcugu ggagauucug gccucagaau uugaucaggc ggacgagaaa     2880

guggaaaucg ucagcguuaa ggccggucuc acgcaucaga ugcggcagga uuucucucug     2940

auuaaaaauc gagacgugaa cgauuaucac caugcuuuug acgcauaccu uaccgccuuc     3000

guggggacuu acuugcugaa gagauaucca aaguuacgcc ccuacuucgu auauggcgag     3060

uuuaaaaagu ucucccaaaa acaggcuaau cucacaaacu uuaacuuccu gugggcccuu     3120

aagcacaaag aucaggucgc aguggaggac accggagaaa agauauggaa uaagccucaa     3180

gaucuggcgu acaucaggca ccuauuugcc uucaaaaaga uucucaucag ucgugagguu     3240

agcgaacggc auggcgcucu guuugaccag acauuguaua aagccucuga ugacaaggca     3300

uccgggcagg gaaguaaaaa gcugauucca gcuaaggaua acagaccgac ugaguuauac     3360

gguggcuauu cagggcagac cagcgccuuc auggcaaucg ugaaagucac gaagggcaaa     3420

aagaccaaau accucguggu uggagugccc acagccgcua uaucgcaacu gcaggguaug     3480

gacguaaagu ccucucagua cacugagaag aucuauaaua uucuuagccc uaaacuguug     3540

ucaaagaacg acgauuuuga agucaucgug ccaaaaguga gguaccgcca gcucauucug     3600

gacaauaacc aacuauucac ccuggggagu gauacauaua aguacaaugc gcgacagcuu     3660

guccucucca acgagguucu ggccgugauc aauagagaca aaucuuuuau agauaagaag     3720

uugagcgaag aggcagaaaa ccaggcucug aaugacgccu ucgaugagau uguaguggcc     3780

auggacaaac acuucgauuu auaugacaag aacggcuuuc gggagaaagu caaggcugga     3840

aaacagaggu ucgaggaacu ccccgugucc cuguuuucag aagauaagga gaaugaaggc     3900

aucacuaaac gcaccguucu uaagcguauc cugcaagggu ugcaugcaaa cgccacgaca     3960

agcgaccuca aggugcugga uggaaaauuc uacaauuga                            3999


<210>  2947
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2947
augaagaacu acaccaucgg ccuggacauu gggguggcca gcgucggaug gguuugcaua       60

gaugagaauu auaaaauccu caacuacaau aacagacacg cuuucggugu gcaugaauuu      120

gaguccgcag aaucugccgc gggcaggcgg cuuaagcgcg ggaugcgacg uagauauaau      180

aggcggaaaa agcgccugca guuguuacaa ucacuguucg acaguuacau uacagauagc      240

ggauuuuucu ccaaaacuga cucucagcac uuuuggaaga acaauaacga guucgaaaau      300

agaucgcuaa cggagguacu cucaagccug aggaucaguu cccggaagua ucccaccauu      360

uaccaucuuc gcucugaucu gaucgagagc aacaaaaaga uggacuugcg acucguguau      420

cuggcucugc acaaucucgu caaauacaga ggccacuuuu uacaggaagg uaacuggucc      480

gaggccgcau cagccgaagg gauggaugac cagcugcuug aguuggugac aagguacgcu      540

gagcuggaaa aucuaagucc ucucgaucug agcgagucuc aauggaaggc agccgaaacu      600

cuucuguuga accggaaucu caccaaaaca gaccagucca aggagcugac cgcuauguuc      660

ggaaaggaau augagccauu uuguaaauua guugccggcc ugggcguguc acuccaucag      720

cuuuucccga gcagugagca ggcgcuggca uacaaggaaa cuaaaacaaa gguccaauug      780

ucuaacgaga acguggaaga gguaauggaa cuacugcucg aggaggaauc cgcccugcuu      840

gaggcuguuc agcccuuuua ucaacaggug guccuguacg aauugcucaa aggggagacc      900

uauguggcca aggcaaaagu gagcgcuuuc aagcaauacc agaaggauau ggccucgcug      960

aaaaauuuac uggacaagac guuuggagaa aaagucuauc guucuuacuu cauauccgau     1020

aagaacucac agcgcgagua ccagaaaagu cacaagguug aggugcuaug caagcucgac     1080

caguucaaua aagaagcgaa guuugccgag acuuucuaua aagaccugaa gaaacuuuug     1140

gaagauaaga gcaagaccuc caucgguaca acugagaaag acgagaugcu gagaauuauc     1200

aaggcaauug auucuaacca guuucuccag aaacaaaagg gcauccagaa ugcugccaua     1260

ccucaucaga acagccugua cgaagcugag aaaauucuua ggaaucagca agcccacuau     1320

ccauucauca ccacagaaug gauugagaag guaaagcaga uccuggcauu ucggaucccc     1380

uacuauauug ggccuuuggu gaaagacacc acgcagucac cauucaguug ggucgaacga     1440

aagggagaug cccccauaac uccuuggaac uuugacgagc agaucgauaa agcugcgucc     1500

gcagaggccu ucauuagccg caugagaaag acauguaccu accucaaagg ccaagaagug     1560

cugccaaagu cuucauuaac uuaugagagg uuugaaguuc ugaaugagcu aaacgguauc     1620

cagcuccgga caaccggggc ugaaagugac uuccgccaua gacugagcua cgagaugaaa     1680

ugcuggauaa uugauaaugu guuuaagcag uacaagacgg ucuccaccaa aaggcuuuug     1740

caggagcuga agaaaucucc guaugccgac gaacucuacg augagcacac aggagaaauc     1800

aaggaggugu ucggcacuca aaaagaaaac gcauuugcca ccucgcugag cggcuauauc     1860

uccaugaagu caauuuuagg ggcuguaguu gacgacaauc ccgccaugac agaggagcug     1920

aucuacugga uugcaguguu cgaagaucgu gagauacuuc acuugaagau ccaggaaaaa     1980

uauccuagua uuacugacgu ccagcggcaa aagcucgcgc uggugaaacu accaggaugg     2040

ggucgauucu cuagacugcu caucgauggc cugccccuug acgagcaggg gcagagcguc     2100

uuggaucaua uggagcagua cuccucagug uuuauggaag uucugaagaa caaaggauuc     2160

ggcuuagaga agaagaucca gaaaaugaau caacaccagg uggacgguac caagaaaauu     2220

agguaugaag auauagagga acucgcuggg agcccugccc ugaagcgcgg caucuggcgg     2280

ucuguaaaaa uugucgagga gcuugugucc aucuuuggag aaccagcaaa cauuguucug     2340

gagguggcca gagaagacgg cgagaagaag aggacaaaaa gucgcaagga ucagugggaa     2400

gaguugacca aaacuacgcu caagaacgac cccgaucuga aaucuuucau cggggagaua     2460

aagagccaag gagaccagcg auuuaaugaa cagcgguucu ggcuauacgu cacacagcaa     2520

gguaaauguc uguacaccgg caaggcucuc gauauccaga accuguccau guaugaggug     2580

gaccauauuc uuccgcagaa uuuuguaaag gacgauucau uggacaaccu ggccuuagug     2640

augccugaag caaaucaacg uaaaaaccag guugggcaga auaagaugcc ccucgagauc     2700

auugaagcua accagcaaua cgccaugaga acucuguggg agaggcuuca cgagcugaaa     2760

cuaaucagua gcggaaagcu cggccgccug aaaaagccau cguucgauga agucgacaag     2820

gauaaauuua uagcgcggca guugguggag accagacaga uuaucaagca ugugagggac     2880

cuguuagaug aacgauucuc caaaucugac auucaccucg ucaaggccgg uaucguuagc     2940

aaauuucgcc gguucucaga gauaccuaag aucagagauu auaauaacaa gcaccaugcu     3000

auggacgcac uguuugccgc ugcccuuauu cagaguaucc uggggaaaua cggcaagaau     3060

uucuuggcau ucgaucucuc caaaaaggac aggcaaaaac aguggcguuc ugugaaggga     3120

agcaacaagg aguuuuuccu guuuaaaaau uucggcaacc uacgccugca gucccccgug     3180

acaggggaag agguaucagg agucgaauau augaagcacg uguacuuuga gcuuccaugg     3240

cagaccacua aaaugacaca aacgggugau ggcauguucu auaaggaaag cauuuuuagu     3300

cccaaaguua agcaggcgaa guacgugucu ccuaaaaccg agaaguucgu ccaugacgag     3360

gugaaaaauc acuccaucug ccucguugaa uuuacuuuca ugaagaaaga gaaggaagug     3420

caggagacaa aauuuauaga ccugaaggua auugaacacc aucaguucuu gaaggagcca     3480

gagagccaac uggccaaauu cuuagcugaa aaggagacca acucgccgau cauccacgcc     3540

cggauuauca gaacaauucc caaauaccag aagauaugga ucgaacauuu uccuuauuac     3600

uucauuucaa cuagggagcu ccacaacgca cgacaguuug aaaucucuua ugagcugaug     3660

gagaaaguca agcaacuuag ugaacggucc agcguggagg aacugaagau uguguucggg     3720

uugcucaucg aucagaugaa ugacaacuac ccaauauaua ccaaaucuag cauccaggau     3780

cgcguccaga aguuuguuga cacgcaacug uacgauuuca aauccuuuga gauuggauuc     3840

gaggaacuaa agaaagcugu ggccgcaaau gcccagagau cagacaccuu uggcaguagg     3900

aucagcaaga agcccaaacc ugaggaagua gcuauugguu augagucuau cacagggcug     3960

aaguaccgga aaccacgauc cguggucggc acuaagagau ga                        4002


<210>  2948
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2948
augagccaga acaucgugga cuacugcauu ggccuggauc ucgggaccgg auccgucggu       60

ugggccguug uggacaugaa ucacagacuu augaagagga acggcaaaca ucuguggggg      120

ucucgguugu ucucaaaugc ugagacagca gccaaccgcc gagcgagucg uagcauaaga      180

aggcgguaua auaagcgcag agaaaggauc cgguuacugc gcgcuauucu acaagauaug      240

guacucgaga acgaccccac uuucuucauc cgacuggaac acacguccuu ucuugaugag      300

gaagacaaag ccaauuaccu gggagcagau uauaaggaca acuacaauuu guucauugau      360

gaggacuuua acgauuauac cuacuaucau aaauacccua caaucuacca ccucagaaag      420

gcccugugug agucuacuga aaaggcugac ccaaggcuga uauaucucgc auuacaucac      480

aucgugaaau accggggcaa uuuccuguau gagggucaga aguuuaacau ggaugccucg      540

aauauugaag accgucuuuc agacgucuuc acccaguuug cugauuucaa caauaucccg      600

uacgaggacg augagaaaaa gaacuuggaa auucuggaga uccuuaaaaa gccccucagc      660

aagaaagcca agguggacga aguuauggcg cugauagcac cugagaaaga uuuuaagagu      720

gccuauaaag aacuugugac agggauugcu ggaaacaaga ugaaugucac caaaaugauc      780

cugugcgagc caauuaagca gggcgacucc gagaucaagu ugaaauucuc ugauagcaac      840

uacgacgauc aauuuuccga aguggagaau gaccucggcg aauauguaga guucaucgau      900

ucacugcaca acauuuacag uuggguugaa uuacagacua uaaugggggc cacacauacc      960

uacaaugcaa gcaucucuga ggcuauggug ucccgcuaua acaagcacca ugaggaccug     1020

cagcuccuua aaaaguguau uaaagacaau guccccaaga aguacuucga uauguuuaga     1080

aacgacucag aaaaacugaa gggauauuac aauuauauca accacccuag caaagcccca     1140

guggaugagu ucuacaagua ugugaaaaag ugcauagaaa aggucgacac gcccgaggcg     1200

aaacagauuu ugcacgauau cgaacuagag aauuuucugc ucaagcaaaa caguaggacu     1260

aaugguucug uuccuuacca gaugcagcug gacgagauga ucaaaauuau cgauaaccag     1320

gccaaguacu auccagugcu uaaagaaaag cgggagcaac uguuguccau ucucaccuuc     1380

aagauaccgu acuauuuugg cccccugaau gaaacaagcg agcaugcaug gaucaaacga     1440

uuagagggga aggaaaacca gcgcauucug ccuuggaacu accaggacau cguagaugug     1500

gacgcuacug ccgagggauu caucaaaaga augaggucgu auuguaccua cuuuccagau     1560

gaagaggucc uacccaaaaa uucucucauu guguccaaau augaaguuua caacgagcug     1620

aauaagauac ggguggacga uaaacuuuug gaggucgaca ucaagaacga cauuuacaau     1680

gaacuguuca ugaacaauaa gacagugacc gagaaaaagc ucaaaaacug gcugguaaau     1740

aaccaaugcu guaauaagaa cgcugaaauc aaaggcuuuc agaaggagaa ucaguucuca     1800

acgagucuua cuccuuggau ugauuuuaca aacaucuucg gugaaauaaa ucagagcaac     1860

uuugaccuga ucgaggauau uaucuaugac uugaccguuu ucgaggauaa gaaaauuaug     1920

aagcgcagac ucaaaaagaa auacgcccug ccagacgaua agaucaagca aauauuaaaa     1980

cugaaguaua aagacugguc caggcuaucu aagaaacucc uggaugggau uguggcagac     2040

aacaaguucg gaagcucagu cacugugcuu gaugucuugg aaaugagucg ucugaaucuc     2100

auggagauca uuaacgaccg ggaucugggc uacgcccaga ugaucgaagc ugcggcaucc     2160

ugccccgagg acggcaaguu uacauauaaa gaagugcagc gauuagccgg gagcccggcu     2220

cugaagagag gaauauggca gucucuucaa aucguugagg agauuaccaa agugaugaag     2280

uguaggccua aauacaucua uauugaauuu gagcgcucag aagagacgaa ggagcggacc     2340

gaaaguaaaa ucaagaaguu ggagaaugua uacaaagacc ucgaugaaca gacaaagguc     2400

gaguacaaaa cugugcugga ggaacuaaag gguuuugaca acaccaaaaa gauaagcucc     2460

gauucucugu uccucuauuu uacacagcug ggcaagugca uguacucggg gaaaaagcua     2520

gacauugaua gcuuggacaa auaucagauc gaucacaucg uuccccaauc ccuggugaag     2580

gacgauucau ucgacaauag agucuuagug guaccaagug agaaccagag gaaacucgau     2640

gaccuggugg uuccuucuga cauucgcguc aagaugaaua gcuuuuggaa acuucuguuc     2700

gaucaugaau ugaucucccc caagaaguuu uacucacuca uuaaaacuga guauaccgaa     2760

cgagacgagg aacgguucau aaaccgucag cugguggaga caagacagau caccaagaau     2820

gugacucaaa uuaucgagga ucacuacagc acgacaaaag ucgccgcaau uagggccaac     2880

cuaucucaug aauuucgcgu uaagaaucac aucuauaaga accgggacau aaaugauuac     2940

caccaugcuc acgacgccua caucguggca cugauuggag gcuucaugag agauagguau     3000

ccaaacaugc augacuccaa agcgguguac agugaguaua ugaagauguu ucgaaagaau     3060

aagaacgaua aaaagcgcug gaaggacggu uucguaauca acagcaugaa uuaccccuau     3120

gaagucgaug gggagcucau uuggaacccu gacaucauaa augaaauucg gaaauguuuc     3180

uacuauaagg auugcuacug caccacuaaa cuggaccaga agucuggcca gauguuuaac     3240

cuuaccgugu ugccaaauga cgcucacucc ccgaaaggaa caaccgaggc cguuaucccc     3300

gugaacaaga auagaaaaga ugucaacaag uacggcgggu ucucaggacu gcaauaugug     3360

aucguugcaa uugaggguaa gaaaaagagg ggcaaaaaga cuaaauuagu gaagaagaua     3420

agugggguac cucuccaccu gaaagccgcu agccuugacg aaaagaucaa auacauugag     3480

gaaaaggaga aucugacaga ugucaaaauc auuaaggacu cgaucccagu gaaccagaug     3540

auagagaugg auggaggcga auaucuacuc acguccccca ucgaguuugu gaauggucgu     3600

cagcuggucu ugaacgaaaa gcagugugcc cugauugcag acaucuacaa ugcuauuuau     3660

aaacaagauu gcgacaacuu agaugacguu cucaugaucc agcuguacau agagcuuauu     3720

aauaagauga aagcccugua uccugcguac cagucuaucg ccgaaaaguu cgagagcaug     3780

accgaggauu acguggcugu aucaaaagaa gagaaggcag acaucauuaa gcagauguug     3840

aucauaaugc aucgcgggcc acggaacggc aaaauucaau augccgauuu uaauguggga     3900

gacagaaucg gcaggaagaa caaaaugagu cucgaccugg aacgagucac uuucgugucc     3960

cagucuccca cagggauuua caccaagaaa uauaagcuau ga                        4002


<210>  2949
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2949
augggcaagg aguacuaucu ggggcucgac aucggaaccg auagcguggg uugggccguc       60

ugcgacacaa acuacaaaau ucccaaguuc aaaggcaaug cuaugugggg gauaagacuu      120

uuugaaaagu ccaacucugc agaggaaagg cggggauucc gcucaagucg acguagaacu      180

cagaggaaac gggagcgccu gaaguuguua gaaaugcugu uugaugagga gaucagcaag      240

auugacccug ccuucuuuau caaacuaaga gaauccaauc ucuaucugga ggauaagucu      300

acgaaaauuc cauacugugu uuucucggac gaaaacuaua ccgauaagga cuuucacagg      360

gauuacccga caaucuauca ucuucggaau gagcugauaa aaucaaacaa gccccacgac      420

gugcgcuugg uauaccucgc gcugcaucac aucauuaaga aucgaggcca uuuccuguuu      480

gauuacagcu auaaaaacga caagaguggu gauuucaugc cuaucuacaa ugaccucaaa      540

aacuauuuuu acgacaauua ugagauugaa uuagauugcu ccgacgugga ugaguucgcu      600

aacacucuga aggacaaauc uaaggggaaa accaagaaga augccgaaau cgcagagcuu      660

uuuggaguca gcaaaaagua cgauaaagug uuguauuccg cccugucacu acucaguggc      720

agcucuguua agcuguucga cauauucgau gacgaagcuc uuaaagaugu ggagaagaag      780

uccgucucau uuagcagugg cuucgacgau aacgcaaaug acuuucaauc uuaccugggg      840

gagagauucg aauugcucga gaaacugaag gccguguacg acugggcugu auuagccgau      900

auucuggacg gaaaagaaua uaucuccgag gcgaagguug caauuuacga aaaacacaag      960

agggaucuca agacacuuaa aaaguauauc aaaaucuaca ugccagagaa gaaagccgag     1020

auuuuuaaca uaagcucgaa ggacaacaau uauguggcuu acucuaagca caucaaaacc     1080

uccaacggua cuggcguccu gauuucaaca uguacccagg aagaguucug cgccuauuug     1140

aagaaaacgc uagggaucug uaaggaugca aguuacgaag agauguuuaa agaaauagag     1200

aauagcacuu ucaugcccaa acaggugacc aaagacaacg gagugguccc uaugcagguu     1260

aaugagaagg aacugguggc uauucucgau aacgccaagg aguaccugcc auuucuucug     1320

aauaaagaca acgauggcaa gacaguauau gacaaaauca aggcguuguu cgaauaccgg     1380

auccccuauu acauuggucc ucucaauacu cauuccacca aaucuuggcu ggugcguagc     1440

aacgagaaga ucuauccaug gaauauugag gaugucgugg acuuugauaa gucagccgaa     1500

aaauucauag agaacuuaac aaguaagugc accuaccugc cgcaauauga cguuaucccc     1560

aaacacuccc uacucuacaa uaaguuuugu gugcugaacg aacuuaauaa cuugaaaauu     1620

gacggggaga agaucagcgu ccagcugaag caggauaucu acaacgaccu cuucaaaaag     1680

uauuacaaag ugacgaauaa gaaacugugc gauuaucuua agucucgcaa cauugcauuc     1740

gacgaaauau caggaaucga uaaugacuuu aagaguagcc ugaaauccua caaggauuug     1800

guagcuuaua accucacuga cgagcagaaa gaggaaauug uuaaggccau cacaauuuuc     1860

ggcgaugaca aaaagcugcu gaaaaagaga cugaagaaug aguuuggcgg gugucuaucu     1920

gaugacgaaa ucaaaaagau augcaaacuc aaguacaccg gauggucgaa acugagcagg     1980

gaguuccuua cugaugugac agcugucuau aaggaaaccg gugaguugau caacauuauc     2040

ugugcccugu gggagacgaa uuacaaccuc accucccugu uauacucaga cgaauuuugc     2100

ugcgacgaua aggagaauaa aacauucaac gacaagauua augaacugaa caguuuuggc     2160

aaugauaaaa cucuuaacca aaucguggag gaauuguaug ucucuccucu cgugaagcgg     2220

ccaauauacc agagccugcu aauuaccaaa gagcuggaga agaucaugaa gugucccccu     2280

aaaaagauuu ucguugaagu ggcacgaggg gccgaggaaa agaagcgcac aguauccaga     2340

aggaauaaac uccuggaccu uuauaaguca ugcaagaacg auuacccauu gcuguuugac     2400

gaguuacaga acaauacuac cgaugacgag cucaaaagcg auaagcugua ucuuuacuuc     2460

acacaguuug gaaaauguau guauaccggc gaaaacaucg agcuggguga cuuguucaau     2520

aagaaccucu acgauauaga ccauaucuac ccccaaucua aaauuaagga cgauucccug     2580

gacaaucggg uccuagugaa gaaaacuacg aacgcuaaga aagauaauga auauccgcug     2640

aguagccaga uccgcgagaa gaugacaccu cacuggaaag cgcuccugga caaggggcuu     2700

auuaacaaaa agaaguacga aagauugguu aggaauuauc cccugucuga ugaggaguua     2760

ucccaguuua ucucacguca acucguggaa acccggcaga guacuaaagc agucgccaac     2820

auacuggagc agcuuuaccc acgaccugac accgaaauug uguauguaaa ggcugcccug     2880

gcaagcgagu ucagacacca guacgauaug cuaaaaugca gggaagugaa ugaccuccau     2940

cacgccaagg augcuuaucu gaacaucguu gucggcaaug ccuacaacgg aaaauucaca     3000

cauaauaagg caaacuuuau caagggcuug caaaacaaug acaaagcgua caccgugaag     3060

cuggauucga uguuaaaaca caacauugac ggggcuugga ucgccgauaa uaacgagucc     3120

cucaauauug ugaagucuac uaugaacaaa aauaacauac gcuauacacg guacgcauuc     3180

gagaagaagg gaggucuguu uaaagucacg auccuuaaga aaggcaaugg gcaggccagc     3240

auuaagaaaa acgacaagag aaauucaauc gaaaaguaug gaggcuacaa ccagguuagu     3300

uccaccuauu ucucuugugu ggagcauggu aaagacaaua acaggaugau uagcauguuu     3360

uccgugaauc uguacgauaa gaacaaauau gaagagaauc ccaucgcuua cuugauagaa     3420

aacuacgggc ucaucaaccc aauuaucaag gucaaaaagg uaaaaauuca gucaugcauc     3480

agcuucgaca auaugcugug ucaccuaggc ggaaaggaua guggcggguc ucaaauaucc     3540

uaucgccccg gagugcagcu gguucuuggu uacgacaacg agcgauauau ucggaaugug     3600

auccguugcg ccggcgggga ucuccacuua cagacuagca agaacgagaa agacaucguc     3660

acagcagaaa agaauauuga guuguuuaac cuguuaauag agaaaaugaa gaauaacauc     3720

uacggagugc ucuauucgaa ucugggcgau aaacuuuacu guucaaagga aaaguucgag     3780

aaacuggaca uuguugaaca gugcaacgug aucaagaaug uauugaccau ucucaaaaac     3840

aaugcuucua agggugaucu gaaaaguauc gggauaaagg ccaacggcgc gaucagacua     3900

aauaacaaca uuuccgagau caagaaaguc aagagcauua aacugaucaa ucaaucugug     3960

acaggacuuu uugaacagga gauagaacuc cugaacuugu ga                        4002


<210>  2950
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2950
augagccaga acaucgugga cuacugcauu ggccuggauc ucgggaccgg auccgucggu       60

ugggccguug uggacaugaa ucacagacuu augaagagga acggcaaaca ucuguggggg      120

ucucgguugu ucucaaaugc ugagacagca gccacucgcc gaaguagccg uuccauaaga      180

aggcgguaua acaagcgcag agaaaggauc cgguuacugc gcgcgauucu acaagauaug      240

guacucgaga aagaccccac guuuuucauc cgacuggaac acaccucuuu ucuugaugag      300

gaagacaagg cuaaauaccu gggaacagau uauaaggaca auuacaacuu guucauugau      360

gaggacuuua augauuauac uuacuaucau aaguacccua ccaucuacca ccucagaaaa      420

gcccugugug agucgacaga aaaggcagac ccaaggcuga uauaucucgc cuuacaucac      480

aucgugaaau accggggcaa cuuccuguau gagggucaga aguuuaauau ggaugcuuca      540

aacauugaag acaaacuuag cgacgucuuc acccaguuug cagauuucaa uaacaucccg      600

uacgaggacg augagaagaa gaauuuggaa auucuggaga uccuuaaaaa gccccucagu      660

aaaaaggcca aaguggacga aguuauggcu cugauauccc cugagaagga auuuaaaucu      720

gccuauaagg agcuugugac ugggauugcg ggaaacaaga ugaaugucac aaaaaugauc      780

cugugcgaga gcauuaagca gggcgauucc gaaaucaaau ugaaguucuc agacaguaac      840

uacgaugacc aauuuagcga gguggaaaac gaucucggcg aguauguaga guucaucgac      900

ucucugcaca auauuuacuc cuggguugaa uuacagacca uaaugggggc aacgcauacu      960

gauaacgccu caaucagcga ggcuauggug agucguuaca auaaacacca ugaagaccug     1020

cagcuccuua agaaguguau uaaagacaac gucccaaaga aauauuucga uauguuucgc     1080

aaugacucug agaaggugaa aggauacuau aacuacauca auagacccaa caaggccccu     1140

guggaugagu ucuauaaguu ugucaaaaag ugcauagaaa aaguugacac cccagaagca     1200

aagcagauuc ugcacgauau cgaguuggag aauuuccuac ugaaacaaaa cuccaggaca     1260

aaugguagcg ugcccuacca gaugcagcuc gacgaaauga ucaagauuau cgauaaccag     1320

gcuaaguauu acccuguacu gaaagagaag cgggaacaac uucugucgau uuugacuuuu     1380

cgaauaccau acuauuucgg cccgcucaau gagaccucug agcacgccug gaucaaacgc     1440

cuggaaggga aggagaacca gagaauuuua cccuggaauu accaggacac aguggauguc     1500

gacgcgaccg ccgaaggauu uaucaaaagg augcgguccu auuguacgua cuucccugau     1560

gaggaagugc ugccaaagaa cucacuaauc guuaguaaau augaggugua caacgagcuc     1620

aauaagauuc gcgucgacga uaagcugcuu gaaguggacg uaaagaacga cauauauaau     1680

gaguuguuua ugaagaacaa aacuguuaca gaaaagaaac ugaagaauug gcucgugaac     1740

aaucaaugcu guagaaagga ugcagagauc aaaggcuucc agaaggaaaa ccaguuuagc     1800

accucccuga cucccuggau ugacuucaca aauaucuuug guaaaauuga ucagucuaac     1860

uucgaccuua ucgagaagau aaucuacgau cugaccgucu ucgaggacaa aaagauuaug     1920

aagaggcguu ugaaaaagaa auacgcucuc ccugaugaca agaucaaaca aauucugaag     1980

uuaaaguaua aagauuggag ccggcuguca aagaaacuac ucgacgggau cguggccgau     2040

aaucgauuug gaaguuccgu cacggugcug gacguucuug aaaugagcag auugaaccug     2100

auggagauaa uuaaugauaa ggaacucggc uacgcucaga ugaucgagga agccucuuca     2160

ugcccaaaag acggcaaguu caccuaugag gagguggcaa aacuggccgg gagucccgcu     2220

uuaaagaggg gaauuuggca gagccugcag aucguagaag agauaacaaa ggucaugaaa     2280

ugucgcccga aguacaucua uauugaauuu gagcgguccg aggaagcgaa agagagaacu     2340

gaaucuaaga ucaaaaagcu agagaacgug uacaaggacu uggaugaaca aaccaaaaag     2400

gaguauaaau cgguucucga ggaacugaag gguuucgaca auacaaagaa gauuagcucc     2460

gauucacuau uucuguacuu cacucagcuc ggcaagugca uguacagugg gaaaaagcug     2520

gacaucgauu cucuugacaa auaucagaua gaucauauug ugccucagag cuuggucaag     2580

gacgauuccu uugacaacag ggugcuggua uuacccucag agaaccaacg caaacucgau     2640

gaccuggugg uuccauucga cauccgagau aagauguacc gguuuuggaa acuucuguuc     2700

gaccacgaau ugaucagccc uaaaaaguuu uauucucuca uuaaaaccga guacacagaa     2760

cgugaugagg agagauucau caauaggcag cuggucgaaa cccgccagau uacuaagaac     2820

gugacgcaga uaaucgagga ccauuauucc acaaccaaag uggcagccau ucgggcuaau     2880

cuaagucacg aauuuagagu caagaaccau aucuacaaga auagggauau uaacgacuau     2940

caccacgccc augaugcaua caucguugcc cugauaggag gcuucaugcg agaccgcuac     3000

cccaauaugc acgauagcaa agcuguguau ucugaguaca ugaagauguu ccggaaaaac     3060

aagaaugacc agaaaaagug gaaagauggu uuugugauca acuccaugaa uuauccauac     3120

gaaguagacg ggaagcucau uuggaacccc gaucugauca augagauuaa gaaauguuuc     3180

uauuacaagg acugcuauug cacuaccaaa cuugaccaaa agucaggcca gauguuuaac     3240

uugacagucc ugccuaauga ugcccauagu gcaaaaggaa ccacugaggc ggugauccca     3300

guuaacaaga acagaaagga cgugaauaaa uacggcgggu ucagcggauu acaauacguc     3360

auagcugcca uugaagguac aaagaaaaag ggcaaaaagc ucgugaaggu uaggaaacug     3420

ucggggaucc cgcuuuaucu gaagcaggca gauaucaaag agcagauuga auacguggag     3480

aaggaggaaa aacuauccga cguaaagaua aucaagaaca auauuccccu caaccagcug     3540

aucgagauug auggacguca auauuugcug acgucuccua ccgaaugugu caaugccaug     3600

caguuagugc ucaacgagga acagugcaaa cugaucgcug acauauacaa ugccaucuau     3660

aagcaggauu uugacggccu ugauaacaug cugaugauuc aauuguaccu ccagcugauc     3720

gacaaacuaa agacucugua uccaauuuac auggguaucg uggagaaauu cgagaaacuu     3780

acagaagauu uugucagcau aucaaaagag gaaaaggcaa auguuauuaa gcagaugcuc     3840

aucaucaugc acaaagggcc ccagaacggc aauauuaucu acgacgauuu caacguggga     3900

aagcgcauag gccggcugaa ugggagaacc uuuuauuugg acaacauuga guucaucagu     3960

caauccccua caggaauuua cacuaaaaag uauaaacugu ga                        4002


<210>  2951
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2951
auguacaccu auuacuucaa ggagagcgaa augguguaug acgucggccu ggauaucggg       60

acaggauccg uugguugggu ggcccucgac gagaacggca aacuugcuag agcaaagggg      120

aaaaaucugg uaggagugag guuguuugau acugcccaga cggcggcuga ccggcgcggc      180

uuccgaacca cacguagaag guuaucucgg cgcaagugga gacugaggcu acucgaugaa      240

cuguuuucag ccgagauuaa cgaaauagac aguagcuucu uucaacggcu uaaauacucc      300

uauguccacc ccaaggauga ggagaauaag gcacauuacu auggugggua ccuguucccu      360

acugaagagg aaaccaaaaa guuucaccgc ucuuacccaa caaucuauca uuugcgacag      420

gagcucaugg cccagccgaa caaaagauuc gacauuaggg agaucuaccu ggcuauucac      480

caucugguga aguaucgggg acacuuucuc ucgucacagg aaaagaucac cauaggcagc      540

acuuacaauc ccgaggauuu agcaaacgcc aucgaaguuu augcugacga gaagggccug      600

aguugggaac uuaauaaccc ugagcaauug acagagauua ucuccgggga agccggauac      660

ggucugaaua agucuaugaa agcggaugag gcacuaaaac ucuucgaauu ugacaacaau      720

caggauaaag uggccauuaa gacccugcuu gcuggccuga cggggaacca gaucgacuuc      780

gccaaauugu uuggaaagga cauaagcgau aaagacgagg caaagcucug gaagcugaaa      840

uuagaugacg aagcucugga ggagaagucc cagacuauuc ucucacaacu uaccgaugaa      900

gagaucgaac uguuccacgc cgucgugcag gcguaugacg gcuucguauu gauuggucua      960

cugaaugggg ccgauagugu uagcgcagcu auggugcagc ucuacgacca gcaucgugag     1020

gaucgcaaac ugcuuaaguc ucuggcccaa aaagcuggau ugaagcacaa gagauuuucc     1080

gaaaucuacg agcagcucgc ccuggcaaca gacgaggcca cuaucaaaaa cggcauuuca     1140

accgcuaggg aauuagucga ggaaagcaau cugaguaagg aggugaaaga ggauacacua     1200

cggcgacucg acgaaaacga guuccugcca aagcagcgca ccaaagcgaa cucugugaua     1260

ccccaucaac uucacuuggc agaacugcag aagauccucc agaaucaggg ccaauauuac     1320

ccuuuucugc uugacacguu cgagaaggaa gaugggcagg acaacaaaau ugaggagcug     1380

uugagauuua ggaucccaua uuacgucgga ccccucguua cuaagaaaga uguggaacac     1440

gccgguggcg acgcugauaa ucauugggua gagcggaacg aaggguucga gaagucccgc     1500

gugacaccuu ggaauuuuga caaagucuuc aacagagaua aggccgcaag ggacuuuaua     1560

gaacgucuga ccggaaauga uacuuauuua auuggcgaga agacacugcc acagaacagc     1620

cuacgguacc aacucuucac cgugcugaau gagcuuaaca auguucgagu gaacgguaaa     1680

aaguuugacu cgaaaacgaa ggccgauuug aucaaugacc uguucaaagc uagaaagacc     1740

gucucucucu ccgcccugaa agauuauuua aaggcacagg ggaagggcga cgugacaauc     1800

acuggacugg cggacgaauc aaaauuuaac aguagccuuu ccucuuacaa ugauuugaag     1860

aaaaccuucg acgcugagua ccucgaaaac gaggauaacc aggaaacacu ggagaagauu     1920

aucgagauuc agacuguauu cgaagacagc aaaauagccu caagggagcu aaguaagcug     1980

ccgcucgaug acgaucaggu uaagaaacug ucccaaaccc acuauacagg cugggggcgc     2040

cuuagcgaaa aguugcugga cucuaaaauc auugaugagc ggggacagaa ggugucaauc     2100

uuagacaaac ucaagaguac cagccagaau uuuaugucca ucauuaacaa ugauaaguac     2160

gguguccagg cauggauaac ugagcaaaac acggggucuu cgaaacugac auucgacgaa     2220

aaggugaaug agcuuaccac uagccccgcc aacaaaagag gcaucaagca guccuuugcu     2280

guccugaaug auauuaaaaa ggccaugaag gaggaaccua ggcgcgugua uuuggaguuc     2340

gcacgagaag accagaccuc aguucggagu gugccacguu acaaccaacu caaagagaag     2400

uaucagucua aaagccuguc cgaagaggcu aagguacuaa agaagacacu ggacggaaau     2460

aaaaacaaga ugucagauga cagauacuuu cucuauuucc agcagcaagg caaggauaug     2520

uacaccggua ggcccaucaa uuuugaacgc cugagccagg acuacgauau ugaccauauc     2580

auaccucagg ccuucacuaa agaugacucu cuugauaacc gggucuuggu guccagacca     2640

gagaaugcga ggaagaguga cagcuuugcc uauacagaug aaguucagaa acaagacggg     2700

ucucugugga cguccuuacu caagucaggc uucaucaacc gcaaaaagua cgagcgacug     2760

accaaggcug gaaaauaucu ugacggccag aagacugggu uuauugcacg gcagcuggug     2820

gagacaagac agaucauuaa aaaugucgcc agucuaaucg aaggagagua cgaaaacagc     2880

aaggcugugg ccauaagguc ggagauuacc gcagauaugc gucuccuggu agguaucaaa     2940

aagcaccgcg aaauuaacuc cuuccaucac gcguuugacg ccuugcugau cacagcugcc     3000

ggccaauaua ugcagaaucg guaccccgau agagacucua cuaacgugua uaaugaguuc     3060

gauagguaca ccaacgacua cuuaaagaau cuccgacagc ugagcucacg ggaugagguu     3120

cgcagacuua aaaguuucgg guuugucgug ggaacgauga ggaagggcaa cgaagacugg     3180

uccgaggaaa auaccucuua ucugcggaaa gugaugaugu ucaagaacau auugacaacu     3240

aaaaagaccg agaaggaucg agguccgcuc aauaaagaga caaucuuuag cccuaagucc     3300

gggaaaaagc ugauuccccu aaacucaaaa agaagcgaca cugcacugua cggcggauau     3360

aguaaugucu acucugcuua uaugacccuu guucgcgcca acggcaagaa ucuccugauc     3420

aagauuccaa ucuccauagc aaaccaaauu gaagugggga auuugaaaau caacgauuac     3480

aucgugaaca auccugccau uaagaaauuc gagaagaucc ugauuagcaa auuaccccuc     3540

ggacagcugg uaaacgaaga cggcaaucuu auauaucugg cuucgaacga guacaggcac     3600

aaugcgaagc aguuguggcu cucaacgaca gaugccgaca aaaucgcauc uauuagugaa     3660

aacuccagcg acgaggagcu gcuagaggcu uacgauaucc ugaccucuga aaaugucaag     3720

aaccguuuuc cauucuuuaa gaaagacauu gauaaacuua gccaggugcg ggacgaguuc     3780

cucgauuccg acaaaagaau cgccguuaua caaacuaucc ugagggguuu gcagauugau     3840

gccgcuuauc aggcacccgu gaagaucauu aguaaaaagg ucucagacug gcauaaguua     3900

cagcaaagcg ggggaaucaa acugucugau aauuccgaaa ugauauacca gucagccaca     3960

ggcauuuuug aaacccgcgu gaagaucagu gaccuccugu ga                        4002


<210>  2952
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2952
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacuaacc aguaugaucu uguuaaaaga aagaugaaag uggcugguaa uuccgacaag      120

aaacaaauaa agaagaacuu cuggggcgua aggcuguuug augacgggca gacggcagug      180

gaucggcgca ugaaucgaac cgcccguaga aggaucgagc ggcgcagaaa caggauuucu      240

uacuugcagg aaaucuucgc ggucgagaug gcuaauauug acgccaacuu uuucugccgg      300

uuaaaugauu cauuuuaugu ggacagugaa aaacgcaaca gccgacaccc uuucuuugca      360

acaaucgagg aagagguugc cuaccauaag aauuauagaa cuauauacca ccugagggag      420

gagcuaguga acuccucuga gaaagcugau cuccggcugg ucuaucuugc acuggcccau      480

aucauuaagu accguggaaa uuucuugauc gaaggcgcuc ucgacaccaa aaacacaucg      540

guggauggug uauacgagca guuuauucaa accuauaauc agguuuucau gucaaacauc      600

gaggaaggga cucuggccaa gguggaggaa aacauagagg ucgcgaauau ucuggcagga      660

aaguuuacac gcagagaaaa auucgagagg auccuccagu uauacccagg cgaaaagagc      720

accggcaugu uugcccaguu cauuagucug aucguggggu ccaaaggaaa cuuccaaaag      780

guguuugacc uuaucgaaaa gacggauauu gagugugcua aggacucuua ugaagaggac      840

uuggaagccc ugcuagcaau aaucggugau gaguacgcug aacucuucgu cgccgcgaaa      900

aauacuuaua acgccguugu gcugagcucc auuaucaccg uaacagcaac ugaaaccaau      960

gcuaaacuuu cagccaguau gauagagcgg uuugacgcuc acgaaaagga ucugggcgag     1020

uugaaagccu ucauuaagcu ccaucugccg aaacaguacc aggaaaucuu uaacaaugca     1080

gccaucgacg gguaugcugg auacauugau ggcaagacaa aacaggugga cuucuacaag     1140

uauuuaaaga ccauccugga gaacauugag ggugcggauu acuuuauagc aaaaaucgaa     1200

gaggaaaauu uccuccgaaa gcaacgcacg uuugacaacg gggccauucc ccaccagcuu     1260

caccuggagg aguuggaagc uaucauccau cagcaagcca aauauuaccc uuuccuaaga     1320

gaggauuaug aaaagauuaa aagccugguc acuuuuagga uaccauacuu cgugggaccc     1380

cucgcaaagg gccagucuga guuugccugg cugacacgga aggcugacgg cgaaauccgc     1440

ccuuggaaua uugaggagaa aguugauuuc gggaaguccg ccguggacuu caucgagaaa     1500

augaccaaca aggacacuua ucuuccaaaa gagaaugucc ugcccaagca cucauugugc     1560

uaccagaagu acauggugua uaacgaacuc acaaaaguaa gauacauuga ugaccaggga     1620

aagaccaauu auuuuagcgg ucaagagaaa cagcagaucu ucaacgaucu guuuaagcag     1680

aaaaggaagg uuaaaaagaa ggacuuagaa cuguuccuac guaacauaaa ucauaucgag     1740

aguccuacga uugagggccu cgaagauucu uuuaacgcau ccuacgcgac cuaucacgac     1800

cugcuuaaag uggggaugaa gcaagagauc uuggauaauc cacugaacac agaaaugcuc     1860

gaggacauug ucaaaauccu gacuguguuc gaagauaagc cgaugauaaa agagcagcuu     1920

cagcaguuua gcgacguccu ggauggaggc guguugaaga agcucgagcg gcgacacuac     1980

accgguuggg ggagacuguc ggcuaaauua cugguuggca uuagggaaaa gcaaucucau     2040

cuaacaaucc ucgacuaucu gaugaaugau gacggacuua accgcaauuu gaugcagcug     2100

auuaacgacu ccaaucucuc auucaaaagu aucauagaga aggaacaggu gagcacuacc     2160

gauaaagacc ugcaguccau cguagccgau uuagcaggcu cucccgccau uaagaagggg     2220

auccugcaaa gccuuaaaau ugucgacgag uuggugucaa ucaugggaua cccuccacag     2280

acaauaguug uggagauggc ucgggaaaac cagaccacug guaagggcaa aaauaacagu     2340

aagcccagau acaaaucccu cgagaaggcc auuaaggaau uugggagcaa aauccugaag     2400

gagcacccua cggauaauca agaacugaaa aacaauaggc uauaucucua cuaucugcag     2460

aacggaaagg acauguacac aggccaggag cuugauaucc auaacuuguc uaauuaugac     2520

auugaucaca ucgucccaca gucauucauu accgacaaca guauagauaa ucugguguua     2580

acuagcuccg cagguaaccg cgagaaaggg ggcgacguac ccccgcucga aaucgugcga     2640

aagcggaaag ucuuuuggga gaagcuguac caaggaaauc uuaugucuaa gcguaaauuc     2700

gauuaucuga ccaaggcuga aagaggcggg uugacagacg ccgacaaagc gagguuuauu     2760

caucgccagc ucguugagac ccggcagauc acuaagaacg uggccaauau ucugcaccag     2820

agauucaaca augaaacaga uaaccacgga aauacgaugg agcaagugag gaucgucacc     2880

cuaaaaucgg cucugguuag ccaguuccga aagcaguuuc agcucuacaa ggugcgcgag     2940

gugaacgacu accaucacgc acaugaugcc uaucugaaug guguagucgc uaacacucuu     3000

uugaaagugu acccucaacu ggaacccgag uucguuuaug gcgaauacca ccaguuugac     3060

ugguucaagg ccaauaaagc aacagcgaag aaacaguuuu auaccaacau aauguuauuc     3120

uuugggcaaa aggagcggau cauugaugaa aacggagaga uccucuggga caagaaauac     3180

cuggagacaa uuaagaaagu gcuugauuau agacagauga auaucgucaa gaaaacugaa     3240

auacagaagg gcgaguucuc caaggccacc auuaaaccaa aggguaacuc aaguaaacug     3300

aucccuagga aggaaaauug ggaccccaug aaauacgggg gccuagauuc uccaaacaug     3360

gcuuacgccg ugaucauuga gcacgcaaag ggaaagaaaa agcucauauu ugagaaaaag     3420

aucauucgua ucacgauuau ggaacgcaaa auguucgaga aggacgaaga ggcuuuucug     3480

gaagagaaag gcuaucggca ucccaagguu uugaccaagc ugccuaaaua cacauuauau     3540

gagugugaaa aggggagaag gcgaaugcuc gccagcgcaa augaggccca gaaaggaaac     3600

caacuggugc uuuccaauca ccugguauca uugcucuacc augcuaagaa cugcgaagcg     3660

agcgauggca aaucucugaa guauaucgag gcccaccggg aaacuuucuc cgagcuacug     3720

gcacagguca gugaguucgc uacccgcuac acacuugccg acgccaaucu cagcaagaua     3780

aacaaucugu uugaacagaa caaagagggu gacauccagg cuauugcaca aucuuucgug     3840

gauuugaugg ccuuuaaugc gaugggggcu ccagccuccu ucaaguuuuu cgaagcaacu     3900

aucgacagaa aaagguauac caaccugaag gaguuacucu caaguacgau uaucuaccag     3960

agcauaacag gacuguacga aucgcggaaa cgacuugaug acuga                     4005


<210>  2953
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2953
augaagaaac ccuacaccau cggccuggac auugggacaa acagcguggg augggccgcu       60

cucacugauc aguaugaccu ugucaagaga aaaaugaagg uugcagguaa uuccgagaaa      120

aagcaaauaa agaaaaaccu guggggcgug agguugguag augaagggaa gacggccgcg      180

caccggcgcg ugaaucgaac cacacguaga aggaucgagc ggcgcagaaa caggauuucu      240

uacuuacagg aaaucuucac ugcugagaug uuugaagucg acgccaauuu cuuuuaucgg      300

cuggaggauu cauucuacau ugagagugaa aaacgccaga gccgacaucc uuucuucgca      360

accaucgagg aagagguggc cuaucacgag aacuacagaa caauauauca ucuaagggaa      420

aagcucguua auuccucuga caaagcugau cugcggcuug uguaccuggc auuggcccac      480

aucauuaagu accguggaaa cuuucucauc gagggcaagc uggacaccaa aaauacuucg      540

gucgaugaag uguucaagca guuuauuaaa acauauaacc aaguauucgc uucagacauc      600

gaggaaggua gccugacccg cauagaggag aauaacgaag uugccaagau uuuuagugag      660

aaacucacga agagagaaaa auuagauaag auccugaauc uuuacccaaa cgagaagucc      720

acugacuugu ucgcgcaguu uauuucucug aucaucggga gcaaaggaaa uuucaagaaa      780

uucuuuaacc uaaccgaaaa gacagauauu gagugcgcaa aagacuccua ugaggaagac      840

cucgaggugc ugcuugccag ggucggcgau gaauacgcug agauauucgu ggccgcaaag      900

aacgcuuaua augccguggu ccugucaagu aucauuacug uuagcaacac cgaaacaaag      960

gcgaaauugu cugccuccau gaucgagcgg uuugacaagc augauaaaga ccucaagcga     1020

augaaagcau ucuuuaaggu gcgccugccg gagaauuuca acgaaguauu uaaugaugug     1080

gagaaggacg gcuacgcugg guauauagaa ggaaaaacca agcaggaggc cuucuacaaa     1140

uacaugaaga aaauguuaga gcacgucgaa ggugcugauu auuuuauuaa ccagaucgag     1200

gaagagaauu uccugagaaa gcaaaggacg uuugacaacg gcgccauccc ccaccagcuc     1260

caucuugagg aacuggaggc aauuuugcac cagcaggcca aguacuaucc uuuccuaaaa     1320

guggauuacg aaaagaucaa aucacugguu acuuuucgga uuccauauuu cguggggccc     1380

cucgcuaaug gacaaagcga guucaguugg cugacacgca aggcggacgg cgaaauaaga     1440

ccuuggaaca ucgaggaaaa agucgauuuu gguaagucug caauugacuu caucgagaaa     1500

augaccaaua aggacacuua ccuuccaaag gagaacgugc ugcccaaaca uuccaugugu     1560

uaucagaagu acaugguaua caaugaauug acaaaaauca gguauaccga ugaccagggg     1620

aagacgcacu acuuuagcgg acaagagaaa cagcagauuu ucaacgaucu cuuuaagcag     1680

aagcguaaag uuaagaaaaa ggaccuggaa uuauuccugu auaauaugaa ccacguggag     1740

ucgccuaccg ucgaaggcgu ggaggaugcc uuuaacucuu ccuucacaac uuaccaugac     1800

cuacaaaaag ucggcgugcc acaggagaua cucgaugacc cgcugaauac cgaaaugcuu     1860

gaggaaauca uuaagaucuu gacaguuuuu gaggauaagc ggaugauuaa cgagcgacug     1920

caggaguucu caaaugugcu cgacgaagcu guacugaaaa agcucgaaag aaggcacuau     1980

acuggguggg gacgccugag ugccaaauug cucaucggua uacgggauaa ggagagccau     2040

cugaccaucu uagacuaccu gaugaacgau gacaaacaca auagaaaccu aaugcagcuc     2100

auuaaugacu ccaaccuguc uuuuaagagc aucauugaaa aggagcaagu cucaacagca     2160

gauaaagaca uccagaguau aguggccgau cuugcuggcu cccccgccau uaagaaaggg     2220

aucuugcaga gccugaagau uguugacgag cucgugggaa ucaugggcua uccuccacag     2280

accauagucg uggaaauggc aagggagaau caaacuacgg guaaagggaa gaacaauucu     2340

aaaccccgcu ucacaucacu ggaaaaggcg aucaaggagu uaggcaguca gauucugaaa     2400

gaacauccua ccgauaacca gggacuuaag aaugaccgau uguaccucua cuaucugcag     2460

aacggcaaag auauguacac ugggcaagag cuagacaucc acaaucugag caacuaugau     2520

auugaccacg uagugccaca guccuuuauc accgauaacu cuauagacaa ucggguucuc     2580

gcuucgagcg ccgcaaaccg ugagaaggga gauaaugucc ccucccugga aguggugaga     2640

aaaaggaagg ucuacuggga gaaacuuuau caggccaaau ugaugucaaa gcgcaaauuc     2700

gacuaccuga caaaggcuga acgggguggc uuaaccgagg ccgacaaagc aagauucauu     2760

cauaggcaac ucguugaaac ucgacagauc acaaagaacg uggcuaauau ccugcaccag     2820

cgcuuuaacu gcaagaaaga ugagaguggg aaugugauug agcagguacg gaucgucacg     2880

cuuaaggccg cgcugguguc ucaauucaga aaacaguuuc aguuguauaa gguuagggaa     2940

gugaacgacu accaucacgc ccacgaugcu uaccucaauu gugucguggc aaacacccug     3000

cuaaaaguuu auccgcagcu ggagccugag uucguguacg gagacuauca ucaauuugau     3060

ugguucaagg ccaauaaggc uacugccaaa aagcaguuuu acacaaacau uaugcuuuuc     3120

uuugcaaaga aggaccguau aaucgaugaa aauggcgaga uucuguggga caaaaaguau     3180

cuugauacca ucaagaaagu auugaacuac cgccagauga auauugucaa gaaaacagaa     3240

auccagaagg gugaguucag caacgcgacu gccaacccca aaggcaauuc cucaaagcug     3300

auaccacgga aagcugacug ggauccuauc aaguaugggg gauuugacgg cagcaacaug     3360

gccuacgcaa uugugaucga acacgagaag aggaaaaaga aaaccgugau uaagaaagaa     3420

uuaauccaaa uaaauauuau ggagagaacg gcuuucgaga aggaccagaa ggaguuccuc     3480

gaagggaaag gauaccgaaa ccccaagguc aucaccaaaa ucccaaagua uacacuguac     3540

gaaugcgaga auggucggcg cagaaugcuu ggcucugcca acgaagcaca gaaagggaau     3600

caaaugguuc ugcccaacca ucuaaugacu cuccuguauc acgccaagaa uugugaggcu     3660

uccgauggaa agaguuuggc guacauugag agccauaggg aaauguuugc cgagcuguua     3720

gacucuauau ccgaauuugc aucacgguau acccucgcug augccaaccu ggagaaaauc     3780

aauacaauuu uugaacagaa caagaguggc gacgugaaag uaaucgccca gagcuucgug     3840

aaucuucugg aguuuaacgc uaugggugcc ccugcaucgu ucaaguacuu ugagacuaau     3900

auugaacgaa aaagauauaa caauuugaag gagcuccuga acgcgaccau cauauaccag     3960

uccaucacgg ggcuauacga agcucgcaag aggcuggaug acuga                     4005


<210>  2954
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2954
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacugauc aguaugaccu uguuaaaaga aagaugaaag uggcugguaa cuccgauaag      120

aaacaaauaa agaagaauuu cuggggcgua aggcuguuug acgaugggca gacggcagug      180

gaccggcgca ugaaccgaac cgcccguaga aggaucgagc ggcgcagaaa uaggauuucu      240

uacuugcagg aaaucuucgc ggucgagaug gcuaacauug augccaauuu cuucugccgg      300

uuaaacgacu cauuuuaugu ggauagugaa aaacgcaaua gccgacaccc uuucuuugca      360

acaaucgagg aagagguugc cuaccauaag aacuauagaa cuauauacca ccugagggag      420

gagcuaguca auuccucuga gaaagcugac cuccggcugg uguaucuugc acuggcccau      480

aucauuaagu accguggaaa cuucuugauc gaaggcgcuc ucgauaccaa aaauacaucg      540

guggacgagg uauacaagca guuuauucaa accuauaacc agguuuucau gucaaauauc      600

gaggaaggug cccuggcgaa gguggaggaa aacacugagg ucgcaagcau acuggccggg      660

aaauuuacac gcagagaaaa guucgagagg auucuccggu uauacccagg agagaaaagu      720

accggcaugu uugcucaguu caucucccug auugugggca acaaggggaa uuuccagaaa      780

guguuuaacc uugucgaaaa gacggauauc gagugugcca aagacucuua ugaagaggac      840

uuggaagcac ugcuagcuau cauuggagau gaguacgccg aacucuucgu ugcggccaag      900

aauacuuaua acgcaguggu acugagcucc auaaucaccg ugacagcuac ugaaaccaau      960

gccaaacuuu cagcuaguau gauugagcga uuugacgccc acgaaaaaga gcugggugaa     1020

uugaaggcau ucaucaaacu ccaucugccg aagcaauacc aggagauauu uaacaaugcc     1080

gagauugaug gcuaugcugg guacaucgac ggaaaaacaa agcaggucga uuucuacaag     1140

uauuuaaaaa ccacgcugga aaacguggag ggcgcggacu acuuuaucac uaagauugaa     1200

gaggagaauu uccuccgcaa acagagaaca uuugauaacg guguuauccc ccaccaacuu     1260

caccuggaag aguuggaagc aauucuacau cagcaggcca aguauuaccc uuuccugagg     1320

gagggguaug acaaaauaaa gagccucgug accuuucgga ucccauacuu cgucggaccc     1380

cuggcuaaug gccaaucuga auuugccugg cuuacucgca aggaugacgg cgagauuaga     1440

ccuuggaaca ucgaggaaaa aguggauuuc gggaaguccg caguagacuu caucgagaaa     1500

augacaaaua aggacaccua ucugccaaaa gaaaacguuu ugcccaagca cucacucugc     1560

uaccagaagu acauggugua uaaugagcug acgaaaauua gguacauaga ugaccaggga     1620

aagaccaacu auuuuagcgg ucgugaaaaa cagcaaaucu ucaacgauuu auuuaagcag     1680

aaacggaagg ucaaaaagaa ggaccuggag cuauuccucc gaaauauuaa ccauaucgag     1740

aguccuacaa uugaaggccu ggaggauucu uuuaaugccu ccuacgcuac uuaucacgac     1800

cuuuugaaag uggggcugaa gcaggaaauc cucgauaacc cacugaauac cgagauacuu     1860

gaagacaucg ucaaaauucu gacaguguuc gaggauaaga gaaugaucaa agagcaguug     1920

gaacaauuua gcgacguucu cgauggagug guacugaaga aguuagagag gcgccacuac     1980

acuggcuggg gucggcuguc ggccaaacua cucgucggga uuagagacaa gcagucucau     2040

cugaccaucc uugauuauuu gaugaacgac gacggccuga auaggaaccu caugcagcug     2100

auaaaugauu ccaacuuauc auucaaaagu auuaucgaaa aggaacaggu gagcacaacc     2160

gacaaagauc ugcaauccau uguugcagac cuugcgggau cuccggcuau caagaagggc     2220

auauugcaga gccucaaaau cguggaugag cuggucucaa uuauggggua ucccccucag     2280

acuaucgugg uagaaauggc ccgcgagaau cagacgacag gaaaggguaa aaacaauagu     2340

aagccacgau acaaaucccu agaaaaggca auuaaggacu uuggcagcca aauccugaaa     2400

gagcacccca ccgauaacca ggaacucaag aauaaccggc uguaccuuua cuauuugcag     2460

aacgggaaag acauauacac uggacaagag cuggauauuc auaauuuauc uaacuaugac     2520

aucgaucaca ucgugccuca gucauucauu accgacaaua guaucgauaa ccucguucug     2580

acaagcuccg ccggcaaucg ugagaagggu ggggacgucc caccccuuga aauugugaga     2640

aaaaggaagg uguuuuggga gaaacuguac cagggcaacu ugaugucuaa gcgcaaguuc     2700

gacuaucuca ccaaagcuga acggggaggc cugacugagg ccgauaaggc aagauuuaua     2760

cauaggcagc uagucgaaac acgacaaauc acgaaaaaug uugcuaacau ucugcaccag     2820

cgcuucaaua aggagaccga caaccacggg aauacuaugg aacaggugcg gaucgugaca     2880

cucaaaucgg cccugguaag ccaguucaga aagcaauuuc agcuuuacaa ggucagggaa     2940

gugaacgauu accaucacgc gcaugacgcc uauuugaaug gaguuguggc uaacacccug     3000

uuaaaagucu acccgcagcu cgagccugaa uuuguguaug gugaguacca ccaguuugau     3060

ugguucaagg caaauaaagc cacagcuaag aaacaauuuu auacuaacau uaugcuguuc     3120

uuugcccaga aggaacguau cauagacgag aacggcgaga uccuuuggga uaagaaauac     3180

cuggaaacca uuaagaaagu ucuagacuau cgccagauca auauugugaa gaaaacggag     3240

auccaaaagg gggaguucuc caaggcaacc auaaaaccca agggaaacuc aaguaaacuc     3300

auuccacgga aggagaauug ggauccugua aaauacggcg gucuggacuc ucccaacaug     3360

gcguacgccg ucaucaucga gcacgcuaag gggaagaaaa agauugugau agaaaagaag     3420

uugauccaga uuaauaucau ggagagaaaa auguuugaaa aggaugagga agccuuccug     3480

gaggagaaag gcuaucauca gccaaaggug uuaacaaagc uccccaaaua cacucuguau     3540

gaaugugaga agggaaggcg acggaugcuu agcuccgcaa acgaagcuca gaaaggcaau     3600

caacuggucu ugucaaacca ccucguuagc cugcuauacc augccaagaa uugcgaggca     3660

ucugacggga aaucccugaa guauauugaa gcccaccgcg agaccuuuag ugagcuucuc     3720

gcucagguga gcgaguucgc gacaaaguac acucuggccg acgcaaacuu gucuaaaauc     3780

aauaaccugu ucgagcagaa uaaggaagga gauauaaaag cuaucgccca guccuuugua     3840

gacuuaaugg ccuucaacgc uaugggcgcc ccugcaucau uuaaguucuu ugaggcgacc     3900

auugauagaa aaagguauac gaaucucaag gaacugcuua guagcacaau cauuuaccaa     3960

ucgaucaccg gucuguacga gucccggaag cgauuggacg auuga                     4005


<210>  2955
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2955
augggcagaa agcccuacau ccugagccuc gacauuggga ccggauccgu ggguuaugcc       60

ugcauggaua aaggcuucaa cguccuuaag uaccacgaca aagaugcucu ggggguuuau      120

uuguuugacg gagcauuaac agcccaggag aggcggcaau uccgcacuuc ucgacguaga      180

aagaauaggc ggauaaaacg ccugggccua cuccaggaac ugcuugcgcc ucuggugcag      240

aacccaaauu uuuaccaguu ccaaagacag uuugcuugga agaacgauaa uauggacuuc      300

aagaacaaau cauugaguga gguacucagc uuucuggguu augaauccaa gaaauacccg      360

acgaucuauc aucugcagga ggcccucuua cugaaggaug aaaaauucga ccccgagcuu      420

auuuacaugg cauuguacca ccuggugaag uauagggggc auuuucuauu cgaucaccuc      480

aagaucgaga aucugaccaa caaugacaac augcaugauu uugucgaacu uauugagaca      540

uacgaaaauc ugaacaauau caaauugaac cucgacuaug agaagacuaa agugauauac      600

gagauccuga aggauaauga aaugaccaaa aacgaccggg ccaagcgcgu uaaaaacaug      660

cagaagaagu uagagcaauu cucuauuaug cugcucggac uuaaauuuaa ugaaggcaag      720

cuguucaacc acgcugacaa ugcagaggaa uugaaaggcg ccaaccaguc gcacacauuc      780

gcugauaauu augaggagaa ccuaaccccu uuucugacug uggaacaguc agaguucauc      840

gaacgagcca auaagauuua ccucagccug acacuucagg acauccugaa agggaagaag      900

aguauggcga uguccaaagu cgcagccuau gauaaguuua gaaacgaguu gaaacaagug      960

aaggacauag uauacaaagc ugauucuacc aggacgcagu ucaagaagau uuuuguuagc     1020

uccaaaaagu cacucaaaca guacgacgcc acuccaaaug aucaaaccuu caguagccug     1080

uguuuauuug accaguaucu gauccggccc aagaaacagu acucucuacu cauuaaggaa     1140

cugaagaaaa ucaucccuca ggauuccgag cuuuauuucg aggcagaaaa cgacacauug     1200

cugaaggugc ucaauacuac cgauaacgcu ucaauuccaa ugcaaauaaa ucuguacgag     1260

gccgaaacaa uccuucguaa ccagcagaaa uaucaugcgg agauuaccga cgaaaugauc     1320

gagaaggucc ugagcuugau acaguuucgc auucccuacu augugggacc ucucgugaau     1380

gaccacacgg ccaguaaauu cgguuggaug gagagaaagu cuaacgaauc caucaaacca     1440

uggaacuuug augaggucgu ugacaggagc aagucggcaa cucaauucau ccggcgaaug     1500

acaaauaagu gcucuuaccu gauuaacgaa gauguguuac cgaaaaauuc ccugcuauac     1560

caggagaugg agguacucaa cgaacugaau gcuacccaga uccgccuuca gacugacccc     1620

aagaacagaa aauauaggau gaugccucaa auuaaguugu uugccgugga gcauauauuc     1680

aaaaaguaca agacagucuc acacaguaaa uuccuggaaa ucaugcucaa uagcaaccac     1740

cgggagaauu uuaugaacca uggcgaaaag cuguccauuu ucgggaccca ggaugacaaa     1800

aaguuugcuu cuaaauuaag cucauaucag gauaugacga agaucuucgg agacaucgag     1860

ggcaagcgcg cccagauuga ggaaauaauc caauggauua ccaucuuuga ggauaaaaag     1920

auucuggugc agaaacuuaa ggaauguuac ccagaguuga caaguaaaca gaucaaucaa     1980

cucaagaagc ugaacuauuc cgguuggggg agacuaagcg aaaaacugcu cacucacgca     2040

uaccagggac auucuauaau cgagcugcuu aggcacucag acgagaauuu cauggaaauu     2100

uugaccaacg auguuuaugg cuuucagaau uucaucaagg aggaaaacca ggugcaaagu     2160

aacaaaauuc agcaucagga caucgccaau cugacaacua gccccgcuuu aaagaaaggc     2220

auauggucca ccauuaagcu cguccgugag cugacaucua ucuuugggga accugagaaa     2280

auuaucaugg aguucgcgac cgaagaucag caaaagggaa aggagcagaa aucgcggaag     2340

cagcuuuggg acgacaacau caaaaagaau aaacuuaaga gcguggauga auacaaguac     2400

auaauugacg uagcaaacaa auugaauaac gagcagcucc aacaggagaa gcuguggcua     2460

uaucuguccc agaaugguaa augcauguac ucaggccaaa guaucgaucu cgacgcccug     2520

cuuucuccaa acgcuacuaa gcacuaugaa guugaucaca uuuuuccccg auccuucauc     2580

aaagacgaua gcauagacaa uaagguguug gucauuaaga aaaugaacca gacgaagggg     2640

gaucaggugc cgcugcaguu uauccaacag ccuuacgaga gaaucgccua uuggaaauca     2700

uuaaauaagg caggacucau uagcgacucu aaacugcaua agcugaugaa gcccgaguuc     2760

acagccaugg auaaagaagg cuucauccag aggcagcugg ucgaaacccg ccaaauuucc     2820

gugcacguuc gggacuuucu aaaggaggag uacccaaaca cuaaagugau accuaugaag     2880

gcuaaaaugg uaagugaguu cagaaagaag uuugauaucc ccaaaauuag gcagaugaau     2940

gacgcccauc acgcaaucga cgcguaucuc aacggugucg uguaccacgg ggcucagcug     3000

gccuacccaa auguugauuu guucgacuuu aacuucaagu gggaaaaagu gcgcgaaaag     3060

uggaaagcac ugggcgaguu uaacaccaag cagaaaagcc gagaauuauu cuuuuucaag     3120

aagcucgaga aaauggaggu cucucaagga gaacggcuga uuuccaagau caaacuugau     3180

augaaucauu uuaagauaaa cuauucacgu aaacuggcca auauccccca gcaguucuac     3240

aaccagacag cugugagucc uaagaccgcc gaguugaagu augaaagcaa uaaaucgaac     3300

gagguagugu acaagggccu cacuccauau caaacauacg uugucgcaau uaaauccgug     3360

aauaagaaag ggaaggaaaa gauggaguau cagaugaucg accacuacgu guucgauuuu     3420

uacaaauucc agaacggaaa ugagaaggaa cuggcucuau aucuggccca aagagagaac     3480

aaagacgaag uccuugaugc gcagauuguu uacucucuca auaaggguga ccuguuguau     3540

aucaacaauc auccguguua cuuugugagc aggaaagagg ugauaaacgc caagcaguuc     3600

gagcugacgg uagaacagca auuaucacuc uauaauguca ugaacaauaa ggagaccaac     3660

guggaaaaac ugcuuauuga guacgauuuu aucgcugaaa agguuaucaa cgaguaucac     3720

cauuaccuga auaguaaauu gaaggagaaa cgcgugcgga cuuucuuuuc cgaaucuaac     3780

cagacacacg aggacuucau uaaggcacuc gaugaacugu uuaaagucgu gaccgccagc     3840

gcuacaagau ccgacaagau aggcucaagg aagaauagca ugacucaccg agccuuccua     3900

gggaaaggaa aggacguuaa aaucgcauac accaguauuu cuggccugaa gacgaccaaa     3960

cccaaguccc uuuuuaagcu cgcggagagc cgcaacgaac uguga                     4005


<210>  2956
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2956
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacugauc aguaugaccu uguuaaaaga aagaugaaag uggcugguaa cuccgauaag      120

aaacaaauaa agaagaauuu cuggggcgua aggcuguuug acgaggggga aacggcagcc      180

gaucggcgca ugaaccgaac cgcgcguaga aggaucgagc ggcgcagaaa uaggauuucu      240

uacuugcagg aaaucuucgc uuuagagaug gccaacauug acgcaaacuu uuucugccgg      300

cugaaugauu cauuuuaugu ggacagugaa aaacgcaaua gccgacaccc uuucuuugcc      360

acaaucgagg aggaagucgc uuaccauaag aacuauagaa cuauauacca ccuaagggag      420

gaacucguua auuccucuga gaaagcagau cugcggcuug uguaucuggc cuuggcucau      480

aucauuaagu accguggaaa cuuccucauc gagggcgccc uggacaccaa aaauacaucg      540

guggauggug ucuacaagca guuuauucag accuauaacc aaguguucau cucaaauaua      600

gaagagggga cucuggcgaa gauggaagag aacacaaccg uagcagacau ucucgccgga      660

aaauuuacgc gcaaggaaaa auuagagaga auccugcagc uuuacccagg cgaaaagagc      720

acuggcaugu ucgcucaguu uauuaguuug aucguugggu ccaaaggaaa cuuccagaag      780

guguucgauc uggucgagaa aaccgacauc gagugugcca aggacucuua ugaagaggau      840

cuagaagcac uccuggcuau uauaggugac gaguacgccg agcuuuuugu ggcggccaag      900

aauacauaua acgcaguggu ccugagcucc aucauuacug uuaccgauac agaaaccaau      960

gcuaaauugu cagccaguau gaucgagagg uucgacgcuc acgaaaagga ucuccgggag     1020

cugaaagccu uuauaaaguu acaucugccg aaacaauacg aagagauuuu cagcaacgug     1080

gcaaucgacg gcuaugccgg guacaucgau ggaaagacga agcagguaga cuuuuacaaa     1140

uaucucaaga cucuucugga gaauauugaa ggcgcugauu acuucaucgc gaaaauugag     1200

gaagagaacu uuuugcgaaa gcagcgcaca uucgacaaug gugcaauacc ccaccagcua     1260

caccuggagg aacucgaggc cauccugcau caacaggcua aauauuaccc auuuuucaag     1320

gaagccuaug auaagauuaa aucucuugug accuuuagaa ucccuuacuu cgucgggccc     1380

cuggcaaacg gacaguccga cuuugccugg uugacuagga aggcugacgg cgagauccgg     1440

ccuuggaaua uugaagagaa aguggauuuc ggcaagucag ccguugacuu cauagagaaa     1500

augacaaaca aggauaccua ucucccaaag gaaaaugugc ugcccaaaca cagcuuauac     1560

uaccaaaagu auauggucua caacgagcug acgaaagugc gcuauaucga cgaucagggg     1620

aagaccaauu acuuuagugg acaggaaaaa cagcaaauuu ucaacgacua uuuuaagaaa     1680

aagagaaagg uaucuaaaaa ggaucuagag caguuccuca ggaauauguc ccauaucgaa     1740

agcccuacaa uugagggucu ggaggacucg uuuaacucuu ccuacgcaac uuaucacgau     1800

cuuuugaaag uuggcaucaa gcaggaagug cuggagaacc cacucaauac cgaaaugcug     1860

gaggacauag ucaaaauccu uacaguguuc gaagauaagc guaugauuaa ggagcagcug     1920

caacaguuuu cagacgucuu ggauggggcg gugcucaaaa agcuggagcg gcgacacuac     1980

acuggauggg gcagauuaag ugcuaaacug cuaguuggua ucagggacaa gcagagccau     2040

cucaccauuc uggacuaccu uaugaacgau gacggguuga aucgcaaccu gaugcagcuc     2100

aucaaugauu ccaaccuguc uuucaaaagc auaauugaaa aggagcaagu gucaacaacc     2160

gacaaggauu uacagaguau cguagccgac cuggcaggcu ccccggccau uaaaaaggga     2220

auccuucaga gcuugaaaau agucgaugaa cucgugucua ucaugggcua ucccccucag     2280

acuauuguug uggagauggc ucgggagaau caaacgacag ucaaggggaa aaacaauuca     2340

agaccaaggu acaagagucu ggaaaaggcc aucaaagagu uuggaagcca gauucuaaag     2400

gaacacccca ccgacaacca ggagcugcgc aauaaccgac ucuaucugua cuaucuucaa     2460

aaugguaaag auauguacac uggccaggaa uuggacaucc auaaccuguc caauuaugau     2520

auagaccaca uugugccuca gucuuucauc accgauaacu cgaucgacaa cuuaguacuc     2580

acaagcuccg cagggaaucg ggagaaggga gacgaugugc caccccugga gauuguucgu     2640

aaaagaaagg ucuuuuggga aaaacuuuuc cagggcaacc ugaugucaaa gaggaaguuu     2700

gacuacuuga ccaaagcuga gcgcgguggg cucacugaag ccgauaaggc gacauucauc     2760

caucggcaac ugguggagac gagacagauu accaaaaaug uggccaacau acuacaccag     2820

agguucaaua acgaaacuga caaucacggc aacaauaugg agcagguccg aaucguuaug     2880

cugaagagug cucucguguc ucaauuucgc aaacaguucc agcuguacaa ggugcgggag     2940

guaaacgauu aucaucacgc acaugacgcc uaccuuaaug gagucguggc uaacacauug     3000

cugaagguuu auccgcaguu agaaccugag uuuguguacg gcgaauauca ccaauucgau     3060

ugguuuaaag ccaauaaggc aaccgcgaaa aagcaguucu acacaaacau uaugcucuuu     3120

uucgcccaga aagagagaau cauugacgaa aauggggaga uccuguggga uaagaaguau     3180

cuugagacua ucaaaaaggu ccuggacuac aggcaaauga acauagugaa aaagaccgaa     3240

auucagaaag gagaguuuag caaggcuacg aucaagccca aagguaacuc cucaaagcua     3300

auuccacgua aagaaaauug ggauccuaug aaguacggcg ggcucgacag ccccaacaug     3360

gccuaugcag uuaucauaga gcacgcuaaa ggaaagaaga aagugguauu cgagaagaaa     3420

auuauccgca ucaccauuau ggaacggaag gccuuugaga aagacgaaaa gucuuuccug     3480

gagaaacagg gcuacagaca gccaaagguc uugacaaagc ugcccaaaua uacuuuauac     3540

gaaugcgaga augguaggcg acggaugcuc gcauccgcca acgaggcuca aaaggggaau     3600

cagcaggucc ugaaaggcca gcuuauaacc cuguugcauc acgcgaagaa cugugaagcc     3660

agugauggaa aaagccucga cuauaucgag ucuaaucgcg aaauguucgg cgagcugcua     3720

gcacaugugu ccgaauuugc uaagagauac acacuggccg augccaaccu uucaaagauu     3780

aaucaacucu ucgagcagaa caaagacaau gauaucaagg ugauugcuca gaguuuuguu     3840

aaccugaugg cauucaaugc caugggggcg ccugcuagcu uuaaauucuu ugaggccacu     3900

aucgaaagga agcgguauac caacuugaaa gagcuguuau cggcaacgau aaucuaccag     3960

uccauuacag gacucuacga agcccgaaag agacuggacg gcuga                     4005


<210>  2957
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2957
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacuaacc aguaugaucu uguuaaaaga aagaugaaag uggcugguaa uuccgacaag      120

aaacaaauaa agaagaacuu cuggggcgua aggcuguuug augacgggca gacggcagug      180

gaucggcgca ugaaucgaac cgcccguaga aggaucgagc ggcgcagaaa caggauuucu      240

uacuugcagg aaaucuucgc ggucgagaug gcuaauauug acgccaacuu uuucugccgg      300

uuaaaugauu cauuuuaugu ggacagugaa aaacgcaaca gccgacaccc uuucuuugca      360

acaaucgagg aagagguugc cuaccaugau aauuauagaa cuauauacca ccugagggag      420

gagcuaguga acuccucuga aaaggcugac cuccggcugg ucuaucuugc acuggcccau      480

aucauuaaau accguggaaa uuucuugauc gaaggcgcuc ucgauaccaa gaacacaucg      540

guggacgagg uauacaaaca guuuauucaa accuauaauc agguuuucau gucaaacauc      600

gaggaaggug cccuggcgaa gguggaggaa aaccuggagg ucgcaagcau acucgccggg      660

aaguuuacuc gcagagaaaa auucgagagg auuuuacggc uguacccagg agagaagagu      720

acaggcaugu uugcucaguu caucucccuu auugugggca auaaagggaa cuuccagaag      780

guguuuaauu uggucgagaa aaccgauauc gaaugugcca aggacucuua ugaagaggac      840

cuggaagcac uacucgcuau cauuggagau gaguacgccg agcuguucgu ugcggccaaa      900

aacacguaua augcaguggu acuuagcucc auaaucacug ugaccgcuac agaaacuaac      960

gccaagcugu cagcuaguau gauugagcga uuugacgccc acgaaaagga uuugggugag     1020

cucaaagcau ucaucaagcu gcauuuaccg aaacaauacc aggaaauauu uaauaacgcc     1080

gcuauugacg gcuaugcggg guacaucgau ggaaagacca aacaggucga cuucuacaag     1140

uaucugaaga caacccucga gaauguggag ggcgcagauu acuuuaucac gaaaauugaa     1200

gaggaaaacu uccuucgcaa gcagagaacu uuugacaaug guguuauccc ccaccaacug     1260

cacuuggagg agcuagaagc cauucugcau cagcaggcua aauauuaccc uuuccucagg     1320

gaggauuaug aaaagauaaa aagccuggug acauuucgga ucccauacuu cgucgggccc     1380

cuugccaagg gacaaucuga guuugcaugg cugacccgca aggccgacgg cgaaauuaga     1440

ccuuggaaca ucgaggagaa aguggauuuc ggcaaguccg cuguagacuu caucgagaaa     1500

augacuaaua aggacacaua uuugccaaaa gaaaacguuc uccccaagca cucacugugc     1560

uaccagaagu acauggugua uaaugaauua accaaaguca gguacauuga ugaccagggg     1620

aagacgaacu auuuuagcgg acaggagaaa caacagauau ucaacgaucu guuuaagcag     1680

aaacguaagg ugaaaaagaa ggaccuagaa cucuuccugc ggaauaucaa ccauauugag     1740

aguccuacca ucgagggucu ugaagauucu uuuaaugccu ccuacgcaac auaucacgac     1800

uugcugaaag ucggcaugaa gcaggagagc cucgauucgc cacugaacgc ggaaaugcuu     1860

gaggacauug ugaaaauccu gacuguuuuc gaagauaagc gaaugauaaa agagcaauug     1920

cagcaguuuu cugacgugcu cgauggggua guccugaaga aguuagagag aaggcacuac     1980

accggauggg gccgccuguc cgcuaaacua cucgugggua uccgggacaa gcagucacau     2040

cugacaauuc uugauuauuu gaugaaugac gacgggcuga acagaaaucu caugcaacug     2100

aucaacgaua guaaucugag cuucaaaucc auuaucgaaa aggagcaggu uucuacuacc     2160

gacaaagauu uacagagcau aguggccgac cuugcaggcu caccggccau uaagaaggga     2220

aucuugcaga gucucaaaau ugucgaugaa cuggugucca ucaugggcua ucccccucaa     2280

acaauaguag uggagauggc uagggagaac cagaccacug uuaaggggaa aaauaacagc     2340

cgcccacgau acaagucucu agaaaaagcc aucaaggagu uuggaucaca gauucugaag     2400

gaacacccca cggacaauca agagcucaaa aacaaucggc uguaccuuua cuauuugcag     2460

aacgguaagg auaucuacac aggccaggaa cuggacauuc auaacuuaag uaauuaugau     2520

aucgaccaca uagucccuca gagcuucauu accgauaacu ccaucgacaa ucucgugcug     2580

acuucuucgg cagggaaccg ugagaaagga ggcgaugugc caccccuuga gaucgucaga     2640

aagaggaaag uguuuuggga aaagcuguac caagguaauu ugaugagcaa acgcaaguuc     2700

gacuaucuca ccaaggcuga gagagggggc cugacagacg ccgauaaagc gcgguuuauu     2760

cauaggcagc uaguugaaac ccgacagauc acuaagaacg uggccaauau ucugcaccag     2820

cgcuucaaca augagacaga caacuacgga aauacgaugg aacaaguacg gauagucacc     2880

cucaaauccg cucugguguc acaguucaga aagcaguuuc agcuuuacaa aguuagggag     2940

gugaacgauu aucaccaugc acacgacgcc uacuugaaug gcgucguggc uaacacucug     3000

uuaaagguuu auccgcaacu cgagccugag uucguguacg gggaauauca ucaguuugau     3060

ugguucaagg ccaauaaagc aacagcgaag aaacaguuuu acaccaacau caugcuguuc     3120

uuugcccaaa aggaacguau uaucgacgag aacggagaaa uucuuuggga uaaaaaguau     3180

cuggagacaa ucaagaaagu acuagacuac cgccagauga auauagucaa gaaaacugag     3240

auccagaagg gugaguucag uaaagcuacc auuaagccca agggcaacuc uagcaaacuc     3300

aucccacgga aggaaaauug ggauccuaug aaauacgggg gacuggacuc ccccaacaug     3360

gccuaugcag ugauuaucga acacgcuaag ggcaaaaaga agauagugau ugagaaaaag     3420

uugauccaga ucaauauuau ggagagaaaa auguuugaaa aggaugagga agccuuccug     3480

gaggaaaaag guuacaggca cccaaagguc uuaacgaaac uccccaagua uacccuguac     3540

gagugugaga aggggcgacg gcgcaugcuu gcaucagcca acgaagcuca aaaaggcaau     3600

cagcugguuu ugagcaacca ucucgugucu cugcuauauc acgcgaagaa uugcgaggcc     3660

uccgacggaa aaagucugaa guacauagaa gcacauagag aggcuuuuag cgaacuucuc     3720

gcccagguau cugaguucgc cacaagguau acucuggcug acgcaaacuu guccaaaauc     3780

aauaaccugu ucgagcagaa uaaggaaggc gauauuaagg ccaucgcgca aucauuugug     3840

gacuuaaugg ucuucaacgc uaugggggcc ccugcaaguu uuaaauucuu ugaggccacc     3900

auugaucgga agcgauacac aaaucucaaa gaacugcuua gcucgacuau cauauaccag     3960

uccaucaccg gacuguauga gucuagaaag cgcuuggacg auuga                     4005


<210>  2958
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2958
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacugauc aguaugaccu uguuaaaaga aagaugaaag uggcugguaa cuccgauaag      120

aaacaaauaa agaagaauuu cuggggcgua aggcuguuug acgaggggga aacggcagcc      180

gaucggcgca ugaaccgaac cgcgcguaga aggaucgagc ggcgcagaaa uaggauuucu      240

uacuugcagg aaaucuucgc uuuagagaug gccaacauug acgcaaacuu uuucugccgg      300

cugaaugauu cauuuuaugu ggacagugaa aaacgcaaua gccgacaccc uuucuuugcc      360

acaaucgagg aggaagucgc uuaccauaag aacuauagaa cuauauacca ccuaagggag      420

gaacucguua auuccucuga gaaagcagau cugcggcuug uguaucuggc cuuggcucau      480

aucauuaagu accguggaaa cuuccucauc gagggcgccc uggacaccaa aaauacaucg      540

guggauggug ucuacaagca guuuauucag accuauaacc aaguguucau cucaaauaua      600

gaagagggga cucuggcgaa gauggaagag aacacaaccg uagcaaacau ucucgccgga      660

aaauuuacgc gcaaggaaaa auuagagaga auccugcagc uuuacccagg cgaaaagagc      720

acuggcaugu ucgcucaguu uauuaguuug aucguugggu ccaaaggaaa uuuccagaag      780

guguucgacc uggucgagaa aaccgauauc gagugugcca aggacucuua ugaagaggac      840

cuagaagcac uccuggcuau uauaggugau gaguacgccg agcuuuuugu ggcggccaag      900

aacacauaua augcaguggu ccugagcucc aucauuacug uuaccgacac agaaaccaac      960

gcuaaauugu cagccaguau gaucgagagg uucgaugcuc acgaaaagga ccucagcgag     1020

cugaaagccu uuauaaaguu acaucugccg aaacaauacg aagagauuuu cucuaaugug     1080

gcaaucgaug gcuaugccgg guacaucgac ggaaagacga agcagguaga uuuuuacaaa     1140

uaucucaaga cucuucugga gaacauugaa ggcgcugacu acuucaucgc gaaaauugag     1200

gaagagaacu uuuugcggaa gcagcgaaca uucgauaaug gugcaauacc ccaccagcua     1260

caccuggagg aacucgaggc cauccugcau caacaggcua aauauuaccc uuuucuuaag     1320

gaagccuaug acaagauuaa aucccuggug accuuccgca ucccauacuu ugucgggccc     1380

uuggcaaaug gacagucaga uuucgccugg cucacuagaa aggcugacgg cgagaucagg     1440

ccuuggaaca uugaagagaa aguggacuuu ggcaagagcg ccguugauuu cauagagaaa     1500

augacaaaua aggacaccua ucugccaaag gaaaacgugu uacccaaaca cagucuguac     1560

uaccaaaagu auauggucua caaugagcua acgaaagugc gguauaucga ugaccagggg     1620

aagaccaacu acuucucugg acaggaaaaa cagcaaauuu uuaaugauua uuucaagcag     1680

aaacgcaagg uauccaagaa agaccucgag caguuucuga gaaacaugag ccauaucgaa     1740

ucgccuacaa uugagggucu ugaggauucu uucaacuccu cauacgcaac uuaucacgac     1800

uugcugaagg uuggcaucaa acaggaagug cucgagaauc cacugaacau ggaaaugcuu     1860

gaggauauag ucaagauccu gaccguguuu gaagacaaaa ggaugauuaa ggagcaauug     1920

cagcaguuca gugggguccu cgauggagcg gugcugaaga aauuagagcg ucggcacuac     1980

acaggcuggg gucgacugag cgcuaagcua cucguuggga ucagagacaa acagucccau     2040

cugacuauuc uugauuacuu gaugaaugac gacggccuga acaggaaucu caugcaacug     2100

aucaacgauu cuaaucugag cuuuaaguca auaauugaaa aagaacaggu gaguaccaca     2160

gacaaggauu uacaguccau cguagccgac cuugcaggaa gcccggccau uaagaaaggc     2220

aucuugcagu cucucaagau agucgaugaa cuggugucaa ucauggggua ucccccucaa     2280

accauuguug uggagauggc ucgcgagaac cagacuacgg ucaaaggaaa gaauaacagu     2340

cggccaagau acaaaagccu agaaaaggcc aucaaggagu ucgguuccca gauucugaaa     2400

gaacacccca cagacaauca agagcucagg aacaaucgcc uguaucuuua cuauuugcag     2460

aacggcaagg auauguacac cgggcaggaa cuggacaucc auaauuuauc uaacuaugau     2520

auagaccaca uugugccuca gucguuuauc acugauaaca gcaucgacaa ucucguacug     2580

accuccucag caggaaaccg agagaaaggc gaugacgugc caccccuuga gauuguucgg     2640

aagcguaaag ucuucuggga aaagcuguuu caagguaauu ugaugaguaa aagaaaguuc     2700

gacuaccuca caaaggcuga gcgcgggggc cugaccgaag ccgauaaagc gacuuuuauc     2760

cauaggcagc uaguggagac acggcagauu acgaagaacg uggccaauau acugcaccag     2820

agauucaaca augaaaccga caaccacgga aauaacaugg agcaagucag gaucguuaug     2880

cucaaaucug cucuggugag ccaguuccga aagcaguuuc agcuuuacaa agugcgcgag     2940

guaaaugauu aucaucacgc acaugacgcc uacuugaacg gcgucguggc uaauacucug     3000

uuaaagguuu auccgcaacu cgaaccugag uucguguacg gggaauauca ccaguuugau     3060

ugguucaagg ccaacaaagc aacagcgaag aaacaguuuu acaccaauau uaugcuguuc     3120

uuugcccaaa aggagcggau cauugacgaa aacggagaga uccuuuggga uaaaaaguau     3180

cuggagacaa uaaagaaagu ccuagacuac agacagauga acaucgugaa gaaaacugaa     3240

auucagaagg gugaguucuc caaagcuacc aucaagccca agggcaauuc aagcaaacuc     3300

auuccaagga aggaaaacug ggauccuaug aaauacgggg gacuggacuc ucccaauaug     3360

gccuaugcag uuaucauaga gcacgcuaag ggcaaaaaga agauuguguu ugagaaaaag     3420

aucauccgua uuacgauaau ggaacgcaaa gccuucgaga aggaugaaaa auccuuuuug     3480

gagaagcagg guuaccggca accaaaagua cugaccaagu uacccaagua uacacucuac     3540

gaaugcgaga acgggagaag gcgaaugcug gcaagugcca augaggcuca gaaaggcaac     3600

cagcaggucc uuaagggaca acugaucacu uugcuccauc acgcgaaaaa uugugaagcc     3660

agcgacggca agucucugga cuauauugag uccaaccggg aaauguucgg ggagcuacug     3720

gcacaugugu cagaguucgc uaaacgcuac acccuugccg augccaaucu caguaagauc     3780

aaccagcugu uugagcagaa uaaggacaac gauauuaaag ugaucgcuca gagcuucguc     3840

aauuugaugg cauuuaacgc caugggagcg ccugcuucgu ucaaguuuuu cgaggccaca     3900

auagaaagaa aaagguauac uaaucugaag gaguuacucc gggcaaccau cauuuaccaa     3960

uccaucacgg gccuguacga agcccgaaaa agacuugacg guuga                     4005


<210>  2959
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2959
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacugauc aguaugaccu uguuaaaaga aagaugaaag uggcugguaa cuccgauaag      120

aaacaaauaa agaagaauuu cuggggcgua aggcuguuug acgaggggga aacggcagcc      180

gaucggcgca ugaaccgaac cgcgcguaga aggaucgagc ggcgcagaaa uaggauuucu      240

uacuugcagg aaaucuucgc uuuagagaug gccaacauug acgcaaauuu cuucugccgg      300

cugaacgauu cauuuuaugu ggacagugaa aaacgcaaua gccgacaccc uuucuuugcc      360

acaaucgagg aggaagucgc uuaccauaag aacuauagaa cuauauacca ccuaagggag      420

gaacucguga auagcucuga gaaagcagau cugcggcuug uuuaucuggc cuuggcucau      480

aucauuaagu accguggaaa cuuccucauc gagggcgccc uggacaccaa aaauacaucg      540

guggauggug ucuacaagca guuuauucag accuauaacc aaguguucau cucaaauaua      600

gaagagggga cucuggcgaa gauggaagag aacacaaccg uagcagacau ucucgccgga      660

aaauuuacgc gcaaggaaaa auuagagaga auccugcagc uuuacccagg cgagaaaagc      720

acuggcaugu ucgcucaguu uauuaguuug aucguugggu ccaaaggaaa cuuccagaag      780

guguucgauc uggucgagaa aaccgacauc gagugugcca aggacucuua ugaagaggau      840

cuagaagcac uccuggcuau uauaggugac gaguacgccg agcuuuuugu ggcggccaag      900

aauacauaua acgcaguggu ccugagcucc aucauuacug uuaccgauac agaaaccaau      960

gcuaaauugu cagccaguau gaucgagagg uucgacgcuc acgaaaagga ucucagcgag     1020

cugaaagccu uuauaaaguu acaucugccg aaacaauacg aagagauuuu cucuaacgug     1080

gcaaucgacg gcuaugccgg guacaucgau ggaaagacga agcagguaga cuuuuacaaa     1140

uaucucaaga cucuucugga gaauauugaa ggcgcugauu acuucaucgc gaaaauugag     1200

gaagagaacu uuuugcggaa gcagcgaaca uucgacaaug gugcaauacc ccaccagcua     1260

caccuggagg aacucgaggc cauccugcau caacaggcua aauauuaccc uuuucuuaag     1320

gaagccuaug auaagauuaa aucccuggug accuuccgca ucccauacuu ugucgggccc     1380

uuggcaaacg gacagucaga cuucgccugg cucacuagaa aggcugacgg cgagaucagg     1440

ccuuggaaua uugaagagaa aguggauuuu ggcaagagcg ccguugacuu cauagagaaa     1500

augacaaaca aggauaccua ucugccaaag gaaaaugugu uacccaaaca cagucuguac     1560

uaccaaaagu auauggucua caacgagcua acgaaagugc gguauaucga cgaucagggg     1620

aagaccaauu acuucucugg acaggaaaaa cagcaaauuu uuaacgacua uuucaagcag     1680

aaacgcaagg uauccaagaa agaucucgag caguuucuga gaaauaugag ccauaucgaa     1740

ucgccuacaa uugagggucu ugaggacucu uucaacuccu cauacgcaac uuaucacgau     1800

uugcugaagg uuggcaucaa acaggaagug cucgagaacc cacugaauac cgaaaugcuu     1860

gaggacauag ucaagauccu gacaguguuu gaagauaaaa ggaugauuaa ggagcaauug     1920

cagcaguuca gugacguccu cgauggggcg gugcugaaga aauuagagcg ucggcacuac     1980

acuggauggg gccgacugag cgcuaagcua cucguuggua ucagagacaa acagucccau     2040

cugaccauuc uugacuacuu gaugaacgau gacgggcuga auaggaaccu caugcaacug     2100

aucaaugauu cuaacuuauc cuuuaaguca auaauugaaa aagaacaggu gaguacaacc     2160

gacaaggauc ugcaguccau cguagccgac cuugcaggca gcccggccau uaagaaagga     2220

aucuugcagu cucucaagau agucgaugaa cuggugucaa ucaugggcua ucccccucaa     2280

acuauuguug uggagauggc ucgcgagaau cagacgacag ucaaagggaa gaacaauagu     2340

cggccaagau acaaaagccu agaaaaggcc aucaaggagu ucggauccca gauucugaaa     2400

gaacacccca ccgacaacca agagcucagg aauaaccgcc uguaucuuua cuauuugcag     2460

aaugguaagg auauguacac uggccaggaa cuggacaucc auaacuuauc uaauuaugau     2520

auagaccaca uugugccuca gucguuuauc accgauaaca gcaucgacaa ccucguacug     2580

acauccucag cagggaaucg agagaaagga gacgaugugc caccccuuga gauuguucgg     2640

aagcguaaag ucuucuggga aaagcuguuu caaggcaacu ugaugaguaa aagaaaguuc     2700

gacuaccuca ccaaggcuga gagggguggg cugacugaag ccgauaaagc gacauuuauc     2760

caucgccagc uaguggagac gcggcagauu accaagaaug uggccaacau acugcaccag     2820

agauucaaua acgaaacuga caaucacggc aacaauaugg agcaagucag gaucguuaug     2880

cucaaaucug cucuggugag ccaguuccga aagcaguuuc agcuuuacaa agugcgcgag     2940

guaaacgauu aucaucacgc acaugacgcc uacuugaaug gagucguggc uaacacacug     3000

uuaaagguuu auccgcaacu cgaaccugag uucguguacg gcgaauauca ccaguuugau     3060

ugguucaagg ccaauaaagc aaccgcgaag aaacaguuuu acacaaacau uaugcuguuc     3120

uuugcccaaa aggagcggau cauugacgaa aauggggaga uccuuuggga uaaaaaguau     3180

cuggagacua uaaagaaagu ccuagacuac agacagauga acaucgugaa gaaaaccgaa     3240

auucagaagg gagaguucuc caaagcuacg aucaagccca aggguaacuc aagcaaacuc     3300

auuccaagga aggaaaauug ggauccuaug aaauacggcg ggcuggacuc ucccaacaug     3360

gccuaugcag uuaucauaga gcacgcuaag ggaaagaaga aggugguauu ugagaaaaag     3420

auuauccgua ucaccauuau ggaacgcaaa gccuucgaga aggacgaaaa auccuuuuug     3480

gagaagcagg gcuaccggca accaaaaguc cugacaaagu uacccaagua uacucucuac     3540

gaaugcgaga augguagaag gcgaaugcug gcaagugcca acgaggcuca gaaagggaau     3600

caacaggugc uuaagggcca gcugauaacc uugcuccauc acgcgaaaaa cugugaagcc     3660

agcgauggaa agucucugga cuauaucgag uccaaucggg aaauguucgg cgagcuacug     3720

gcacaugugu cagaauuugc uaaacgcuac acacuugccg augccaaccu caguaagauu     3780

aaucagcugu uugagcagaa caaggacaau gauaucaaag ucauugcuca gagcuucguu     3840

aacuugaugg cauuuaaugc caugggggcg ccugcuucgu ucaaguuuuu cgaggccacu     3900

aucgaaagaa aaagguauac caaccugaag gaguuacucu ccgcaacgau aaucuaccaa     3960

ucuauuacag gacuguacga agcccggaaa cgacuugacg gcuga                     4005


<210>  2960
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2960
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacuaacc aguaugaucu uguuaaaaga aagaugaaag uggcugguaa uuccgacaag      120

aaacaaauaa agaagaacuu cuggggcgua aggcuguuug augacgggca gacggcagug      180

gaucggcgca ugaaucgaac cgcccguaga aggaucgagc ggcgcagaaa caggauuucu      240

uacuugcagg aaaucuucgc ggucgagaug gcuaauauug acgccaacuu uuucugccgg      300

uuaaaugauu cauuuuaugu ggacagugaa aaacgcaaca gccgacaccc uuucuuugca      360

acaaucgagg aagagguugc cuaccaugau aauuauagaa cuauauacca ccugagggag      420

gagcuaguga acuccucuga aaaggcugac cuccggcugg ucuaucuugc acuggcccau      480

aucauuaaau accguggaaa uuucuugauc gaaggcgcuc ucgauaccaa gaacacaucg      540

guggacgagg uauacaaaca guuuauucaa accuauaauc agguuuucau gucaaacauc      600

gaggaaggug cccuggcgaa gguggaggaa aaccuggagg ucgcaagcau acucgccggg      660

aaguuuacuc gcagagaaaa auucgagagg auuuuacggc uguacccagg agagaagagu      720

acaggcaugu uugcucaguu caucucccuu auugugggca auaaagggaa cuuccagaag      780

guguuuaauu uggucgagaa aaccgauauc gaaugugcca aggacucuua ugaagaggac      840

cuggaagcac uacucgcuau cauuggagau gaguacgccg agcuguucgu ugcggccaaa      900

aacacguaua augcaguggu acuuagcucc auaaucacug ugaccgcuac agaaacuaac      960

gccaagcugu cagcuaguau gauugagcga uuugacgccc acgaaaagga uuugggugag     1020

cucaaagcau ucaucaagcu gcauuuaccg aaacaauacc aggaaauauu uaauaacgcc     1080

gcuauugacg gcuaugcggg guacaucgau ggaaagacca aacaggucga cuucuacaag     1140

uaucugaaga caacccucga gaauguggag ggcgcagauu acuuuaucac gaaaauugaa     1200

gaggaaaacu uccuucgcaa gcagagaacu uuugacaaug guguuauccc ccaccaacug     1260

cacuuggagg agcuagaagc cauucugcau cagcaggcua aauauuaccc uuuccucagg     1320

gaggauuaug aaaagauaaa aagccuggug acauuucgga ucccauacuu cgucgggccc     1380

cuugccaagg gacaaucuga guuugcaugg cugacccgca aggccgacgg cgaaauuaga     1440

ccuuggaaca ucgaggagaa aguggauuuc ggcaaguccg cuguagacuu caucgagaaa     1500

augacuaaua aggacacaua uuugccaaaa gaaaacguuc uccccaagca cucacugugc     1560

uaccagaagu acauggugua uaaugaauua accaaaguca gguacauuga ugaccagggg     1620

aagacgaacu auuuuagcgg acaggagaaa caacagauau ucaacgaucu guuuaagcag     1680

aaacguaagg ugaaaaagaa ggaccuagaa cucuuccugc ggaauaucaa ccauauugag     1740

aguccuacca ucgagggucu ugaagauucu uuuaaugccu ccuacgcaac auaucacgac     1800

uugcugaaag ucggcaugaa gcaggagagc cucgauucgc cacugaacgc ggaaaugcuu     1860

gaggacauug ugaaaauccu gacuguuuuc gaagauaagc gaaugauaaa agagcaauug     1920

cagcaguuuu cugacgugcu cgauggggua guccugaaga aguuagagag aaggcacuac     1980

accggauggg gccgccuguc cgcuaaacua cucgugggua uccgggacaa gcagucacau     2040

cugacaauuc uugauuauuu gaugaaugac gacgggcuga acagaaaucu caugcaacug     2100

aucaacgaua guaaucugag cuucaaaucc auuaucgaaa aggagcaggu uucuacuacc     2160

gacaaagauu uacagagcau aguggccgac cuugcaggcu caccggccau uaagaaggga     2220

aucuugcaga gucucaaaau ugucgaugaa cuggugucca ucaugggcua ucccccucaa     2280

acaauaguag uggagauggc uagggagaac cagaccacug uuaaggggaa aaauaacagc     2340

cgcccacgau acaagucucu agaaaaagcc aucaaggagu uuggaucaca gauucugaag     2400

gaacacccca cggacaauca agagcucaaa aacaaucggc uguaccuuua cuauuugcag     2460

aacgguaagg auaucuacac aggccaggaa cuggacauuc auaacuuaag uaauuaugau     2520

aucgaccaca uagucccuca gagcuucauu accgauaacu ccaucgacaa ucucgugcug     2580

acuucuucgg cagggaaccg ugagaaagga ggcgaugugc caccccuuga gaucgucaga     2640

aagaggaaag uguuuuggga aaagcuguac caagguaauu ugaugagcaa acgcaaguuc     2700

gacuaucuca ccaaggcuga gagagggggc cugacagacg ccgauaaagc gcgguuuauu     2760

cauaggcagc uaguugaaac ccgacagauc acuaagaacg ugacaaauau ucugcaccag     2820

cgcuucaaca augagacgga caacuacgga aauaccaugg aacaaguacg gauagucacu     2880

cucaaauccg cccugguguc acaguucaga aagcaguuuc agcuuuacaa aguuagggag     2940

gugaacgauu aucaccaugc ucacgacgca uacuugaaug gcgucguggc caacacacug     3000

uuaaagguuu auccgcaacu cgagccugag uucguguacg gggaauauca ucaguuugau     3060

ugguucaagg cuaauaaagc caccgcaaag aaacaguuuu acacaaacau caugcuguuc     3120

uuugcgcaaa aggaacguau uaucgacgag aacggagaaa uucuuuggga uaaaaaguau     3180

cuggagacua ucaagaaagu acuagacuac cgccagauga auauagucaa gaaaaccgag     3240

auccagaagg gugaguucag uaaagccacg auuaagccca agggcaacuc uagcaaacuc     3300

aucccacgga aggaaaauug ggauccuaug aaauacgggg gacuggacuc ccccaacaug     3360

gcuuaugccg ugauuaucga acacgcaaag ggcaaaaaga agauagugau ugagaaaaag     3420

uugauccaga ucaauauuau ggagagaaaa auguuugaaa aggaugagga ggcuuuccug     3480

gaagaaaaag guuacaggca cccaaagguc uuaaccaaac uccccaagua uacacuguac     3540

gagugugaga aggggcgacg gcgcaugcuu gccucagcaa acgaagccca aaaaggcaau     3600

cagcugguuu ugagcaacca ucucgugucu cugcuauauc acgcuaagaa uugcgaggcg     3660

uccgacggaa aaagucugaa guacauagaa gcccauagag aggcauuuag cgaacuucuc     3720

gcucagguau cugaguucgc cacuagguau acccuggccg acgcuaacuu guccaaaauc     3780

aauaaccugu ucgagcagaa uaaggaaggc gauauuaagg caaucgccca aucauuugug     3840

gacuuaaugg ucuucaacgc gaugggggcu ccugccaguu uuaaauucuu ugaggcaaca     3900

auugaucgga agcgauacac uaaucucaaa gaacugcuua gcucgaccau cauauaccag     3960

uccaucacgg gacuguauga gucuagaaag cgcuuggacg auuga                     4005


<210>  2961
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2961
augaagaaag aguacaccau cggccuggac auugggacaa acagcguggg augggccguc       60

cucacugaaa auuaugaucu uguuaagaaa aagaugaaag uguacgguaa cacggagacc      120

aaguaucuga agaaaaauuu guggggcgua agauuauucg acgaagggga gacagcugca      180

gauaggcggc ugaagcgcac uacccgacgu agauacucca ggcggcgcaa cagaauaugc      240

aggcuacagg accucuuuac agaagagaug aaucaagugg augccaacuu cuuucaccgg      300

cugcaggagu cuuuccuugu ccccgacgaa aaagaguuug aacgccaugc gaucuucgga      360

aagauggagg aggaaguguc auauuaccga gaguuuccua ccauuuauca ccugagaaaa      420

cauuuggcug auacuagcga acaggccgac cucaggcugg uuuaccuggc acucgcccac      480

aucgugaagu accggggcca uuucuuaauu gagggugaac ugaauacaga gaacagcucc      540

gucucugaga ccuuucguac guucauccag guguauaauc aaauauuucg cgaaaacgag      600

guaccacuug cuguuccgga uaauaucgaa gaguuguucu cggaaaaggu gucaagagca      660

aggaaagucg aggccauucu gagcguguac agugaggaaa aguccacugg gacccuagcu      720

caguuucuca aacugauggu gggaaaccag ggccgguuca agaaaacauu cgaccuugag      780

gaagauggca ucauucagau ccccaaggag gaauaugagg aggaacugga gacuuugcuc      840

gccauaauug gggacgaaua cgcggagauc uuuucugcaa ccaaaagcgu cuaugaugcc      900

guugcucugu ccggaauuuu aucagugaca gacggugaca ccaaggccaa gcugagugca      960

agcaugguag agcgauacga agcucaccaa aaagaucucg ugcaguucaa gcaguuuauc     1020

cgcaaagagc uuccugaaau guaugcccca aucuucagag acaauucugu cuccggcuac     1080

gcgggguacg uggagaacuc aaagguugug acgcaggccg aauuuuauaa auacauuaag     1140

aaggcaauag agaaaguccc cggagcugag uauuuccugg aaaagaucga gcaagaaacu     1200

uucuuggaua aacagaggac auucaauaac ggcgugauuc cucaccagau ccaucuagag     1260

gaacuggagg ccauaauuca aaagcaggcu accuacuauc cauuucucgc cgacaacaaa     1320

gaggaaauga agcagcuggu aacuuuccgg auccccuacu auguuggucc ucuugcagau     1380

gggaauagcc cauuugccug gcuggagcgc aucaguucug aaccgauuag acccggaaac     1440

uuggcugagg uggucgacau caagaaaucc gcgacaaagu ucauugaaag gaugaccaau     1500

uuugauacgu accucccuac cgagaaagug cugccaaagc acagcaugau auacgagaaa     1560

uauauggucu acaacgaauu aacaaaggug ucguauguug acgagcgugg caugaaucag     1620

cgguucucug gcgaagagaa gaaacaaauc guggaggaac uguucaagca gucccgaaaa     1680

guaacuaaga aacuacucga gaaguuucug ucaaacgaau uugggcuugu cgauguggca     1740

auuaaaggaa ucgagaccag uuuuaaugcc gguuacggca cauaucauga cuucuugaag     1800

aucgggauua cuagagaaca gcuggauaag gaggagaaca gcgaaacccu cgaggaaaua     1860

guuaaaaucc ugacaguguu ugaggacagg aagaugauuc gcgaacagcu uaaaaaguac     1920

accuaucugu ucgacgagga ggucuugaaa aagcucgaac ggagacacua cacuggaugg     1980

ggcaggcugu ccgcuaaguu acugaucggu auuaaagaga agcgcacgca caaaacaauc     2040

cuagauuacc ucauagaaga cgaugggggc aagcaaccca ucaaucgaaa ccugaugcag     2100

cuuauuaaug acucugauuu gagcuuuaaa ucagagaucg ccgaagcaca gagugacaug     2160

aacaccgagg aucugcauga ggugguacag aaucucgccg gauccccugc uauuaagaag     2220

ggcauccugc aaaguuuaaa aauaguggac gaacugguug auauuauggg gucucuucca     2280

aagaacaucg ucguggagau ggcccgggaa aaucagacua ccucacgugg aagaacaaac     2340

aguaacccca ggaugaaagc auuggaggag gcgaugcgca aucuccggag caaccugcua     2400

aaggaauauc cgaccgacaa ucaggcucug cagaacgaua gacucuaccu guauuaccuu     2460

caaaauggua aagacaugua uacuggcuug gaucuguccu uacacaaccu cucuucguac     2520

gacauugacc auaucgugcc ucagagcuuc acaacggaua auucccugga caacaggguc     2580

cuuguuucaa guaaggagaa ucgagggaag aaagaugacg ugcccucuaa ggaaguggua     2640

cagaaaaaca uaacccugug ggagacuuug aagaauagca accucaucuc ccaaaagaag     2700

uaugauaauc ugacaaaagg acuacgcggc ggucugaccg aagacgaucg ggcccacuuu     2760

auuaagagac agcucgucga gacaaggcag aucacuaagc auguggcacg uauucuggac     2820

cagcgcuuca acucacaaaa agaugaggaa gggaagacca uccgggccgu uagagugguc     2880

acgcuuaaaa gcucuuugac cucccaguuu aggaagcagu ucgcuauaca caaagugcga     2940

gagauuaaug acuaccacca uggccacgau gccuaccuga acggaguugu ggcaaacagu     3000

uuacuccggg uauauccaca gcugcaaccu gaauuugucu acggcgacua ucccaaguuc     3060

aaugcuuaca aggccaacaa agcgacagcc aagaaacagc uuuauacuaa uaucaugaag     3120

uucuuugcug aggaugcagu gaucauugac gaaaacgggg agauccugug ggacaaaaag     3180

aauauugcca ccgugaagaa agucaugagc uacccacaga ugaacauagu uaagaaaccc     3240

gagauccaga caggaucuuu cuccaaggaa acuauuaaac cuaaggguga uucagacaag     3300

cuaaucaguc gcaaaaccaa uuggagccca aagcucuaug gcggguuuga uucgccgcaa     3360

guggcuuacu ccguaauuau cacguacgag aaaggaaaga aaaaggugag agccaaggca     3420

auagucggca ucacaauuau ggaacagucu cuguucaaaa aggaccccgu gagcuugcug     3480

gaggaaaaag guuaugcgaa cccugagguu uuaauccauc ucccaaagua cacccuguau     3540

gagcuugaaa augggaggcg gcgacuguug gccucagcua acgaggccca gaaaggcaau     3600

caacucgugc ugcccgcaag ucuagucacu cugcuuuacc acgcuaaaca gguggaugaa     3660

gacuccggaa aaucugagga guauguuaga gaacaccgcg ccgaguuugc agaaauucuc     3720

aacuacgugc aggccuucag cgagacaaag auccuggcua auaagaacuu gcagaccaua     3780

cugaaauuau augaagagaa uaaggaggcg gauauuaaag aaaucgccga guccuuuguc     3840

aaccucauga aguucucagc auacggcgcu ccuauggacu uuaaauucuu ugggaagacu     3900

aucccaagga gccguuacac caguguagga gaacugcuuu cugccacaau uauaaaccaa     3960

uccaucacgg gccuguauga gacccggaga aaguuggugg auuga                     4005


<210>  2962
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2962
augagaaagc ccuacaccau cggccuggac auugggacaa acagcguggg augggccguc       60

cucacugauc aguauaaucu uguuaaaagg aagaugaaag uggcugguuc cgcagagaag      120

aaacaaauaa agaagaacuu cuggggcgua cggcuguuug acgaagggga gguggccgcg      180

ggacgccgaa ugaaucguac gaccagaagg cggaucgaac gcagaaggaa ccggauugcu      240

uacuugcagg agaucuucgc cgcagaaaug gccgaggucg augcuaauuu cuucuaucgc      300

uuagaggacu cuuuuuacau ugaaucagag aaacgacaca guagacaucc uuucuuugca      360

acaaucgaag aggagguggc cuaucacgaa gaguacaaga cuauauauca ucugagggaa      420

aagcuaguua acagcuccga uaaagcugac cuccggcugg uguaccuugc ccuggcgcac      480

aucauuaaau accguggcaa uuucuugauc gaggguaugc ucgauaccaa gaacacaucu      540

gucgacgaag uguuuaagca guucauucag accuauaauc aaaucuuugc aucggauaua      600

gaggaggggu cacugacucg ccuggaagag aacaaagaag uagccgagau ucucagcgaa      660

aaguuaacaa gaagggagaa acuggacaag auccuuaaau uguacaccgg agagaagagu      720

acgggcaugu ucgcucgguu uauuaaucug aucaucggcu ccaaagggga uuucaagaag      780

guuuucgacc uagaugaaaa agccgagauu gaaugcgcaa aggacacuua ugaggaagac      840

cucgaggcuc ugcuugccaa aauaggagau gaguacgcgg aaaucuuugu ggccgcaaag      900

ucuaccuaua acgcugucgu gcugagcaau auuaucacag ugacugacac cgagacaaaa      960

gccaaguugu ccgcuucaau gauagaacga uucgauaagc augccaaaga ccucaagcgc     1020

cugaaagcau uuuucaagau gcaguuacca gagaaauuua acgaggucuu caaugauauu     1080

gaaaaggacg guuacgccgg cuauaucgau gggaagacca cgcaggagaa auuuuacaag     1140

uacaugaaaa agaugcuggc uaacaucgac ggagcggauu auuucauuga ccagaucgaa     1200

gaggaaaacu uucucagaaa acaaaggacu uucgauaaug gcacaauucc gcaccagcuu     1260

caccuggagg aguuggaagc aauacuacau cagcaggcca aguacuaucc cuuucugcgg     1320

aaggacuacg agaaaauccg cagucucguu accuucagaa ucccuuauuu uauuggucca     1380

cuggcuaacg ggcaaagcga cuucgccugg cuuacuagga aggcagaugg agaaauccgu     1440

cccuggaaua uugaggaaaa aguggacuuc ggcaagucug ccauagauuu uaucgagaaa     1500

augacaaaca aggacaccua ccugccuaaa gagaauguau ugccaaagca cucccucugu     1560

uaucagaagu acauggugua caacgaacug acgaaaauuc gguauaucga ugaccagggc     1620

aagacccauc acuucucagg gcaagagaaa cagcagauuu uuaauggauu auucaagcag     1680

caacgaaaag ucaagaagaa agaucuggaa agauuucuau acacaaucaa ccacauagag     1740

agccccacua ucgaaggugu ggaggacgcu uucaauaguu cuuuugccac cuaucaugau     1800

cuccagaagg gcgggguuac acaggagauu cuggacaacc cucuuaaugc agauauguug     1860

gaagagaucg ugaaaauucu gacugucuuc gaagacaaga ggaugaucaa agagcagcuc     1920

caauccuuua gcgaugugcu ggacggaacc auacuuaaga agcuggagcg ccggcacuac     1980

acaggcuggg guagauuguc ggcgaaacuc cugaccggga uuagggacaa gcauucucac     2040

uuaacuaucc uggauuaucu aaugaacgac gauggccuca aucgcaaccu gaugcagcuu     2100

auuaaugacu ccaacuuguc auucaaaagu aucauagaaa aggagcaggu aagcacggcu     2160

gauaaaggaa uccaguccau uguugccgaa cuggcaggcu cuccagccau caagaagggg     2220

auucuccaaa gccugaaaau cguggacgag uuagucggaa uaauggguua cccgccccag     2280

acaauugugg ucgaaauggc ucgagagaac cagaccacug gcaaggggaa aaauaacuca     2340

aagccucggu uuaucagucu ggagaaagcc aucaaggaau uuggauccca gauucuuaaa     2400

gagcauccaa ccgauaauca augcuugaag aacgaccguc ucuaucugua cuaccuacag     2460

aauggcaagg auauguauac agguaaagaa cuggacaucc acaaccucag caauuacgau     2520

auugaccaca uaauccccca gucuuuuauu accgauaacu caaucgacaa uagagugcug     2580

guuaguagca cugcaaacag ggagaagggg gacaaugugc cucuuuugga aguaguccgc     2640

aaacggaagg cuuucuggga gaaacuguau caagccaagu uaauguccaa gagaaaauuc     2700

gauuaccuca caaaggcgga gaggggcgga cugacggaag ccgacaaagc uaacuuuauu     2760

cagcgacagc uuguggagac ccgccagauc acuaagaaug uugcaaacau acuguaucaa     2820

cgguucaauu guaaacagga ugaaaacggc aacgaggugg aacaggucag aaucgugaca     2880

uugaagucua cccucguauc gcaguuuagg aagcaauucc agcuguacaa agugcgugag     2940

guuaaugacu aucaucacgc ccaugaugcu uaccuaaacg gggucguggc caauacacug     3000

cucaaggugu acccacagcu ggagcccgaa uuugucuaug gagacuacca ccaguucgau     3060

ugguuuaaag caaacaaggc gacugccaaa aagcaauucu auaccaauau uaugcgcuuu     3120

uucgcuaagg agaaccagau cauugacaaa aauggugaaa uccuuuggga uaaccgguac     3180

uuggacacga uaaagaaagu ucugagcuau agacagauga auauugugaa gaaaaccgag     3240

auccaaaagg gcgaguuuuc caacgccaca gugaauccga aggggaacuc aaguaaauua     3300

aucucuagga aggcagauug gaauccuauu aaauacggag gcuucgacgg uagcaacaug     3360

gcuuauucca uaguaaucga auacgagaag cggaaaaaga aaacugucau uaagaaggaa     3420

cucauccaga uuaauaucau ggagcgagug gccuucgaaa aagaccagaa ggcauuucug     3480

gaggagaaag gguacuauuc acccaagguu cuuaccaaaa uaccaaagua cacacuguau     3540

gaaugcgaga acggccgcag aaggaugcua ggaagcgcca acgaagcuca gaagggcaau     3600

caaauggugc ucccuaacca ccugaugacu uugcuguacc augcgaaaaa uugugaggcc     3660

aacgaugggg aaucuuuagc auauaucgag augcaccggg agauguucgc ugaacuccug     3720

gccuacauuu ccgaguuugc caagcgauau acccuugcua augacagacu ggagaaaauc     3780

aacauguucu uugaacagaa uaagaaagga gauauuaagg ucaucgcaaa gaguuucgac     3840

aaauugaagg uguuuaacgc cuucggcgcg ccccgcgauu uugaguucuu ugaaacgaca     3900

auaaaaagga agcguuacua caauauuaaa gagcuccuga acgcuaccau caucuaucag     3960

agcauuacug gucuauacga agcccggaag agacuggagg acuga                     4005


<210>  2963
<211>  4008
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2963
augaagaacu acaccaucgg ccuggacauu gggacaaaua gcgugggaug ggccgucaua       60

aaagaugacc ucacucuugu uagaaagaaa aucaagauuu ccgguaacac ggauaaaaag      120

gaggugaaga aaaaucugug gggcucuuuc uuguuugaac agggggacac cgcucaagau      180

acaaggguaa agcggaucgc acgccgacgu uaugagagaa ggcgguuccg cauuagagaa      240

uuacagaaaa ucuuugacaa gucaauggga gagguggaua guaacuucuu ucacaggcug      300

gacgagagcu uccuagucga ggaggauaaa gaauacucca aguaucccau auuuucuaau      360

gagaaggaag acaaaaacua cuaugauaag uacccuacua ucuaucaucu ccggaaagac      420

cuggccgauu cgaaucagaa ggcggaccuu cgccugauuu acuuggcucu cgcccacaug      480

aucaaauacc gaggccauuu ccugauugag ggugaccuga agauggaugg gaucucaaua      540

agcgagaguu uucaggaguu cauugacucc uauaacgaag ugugcgcacu cgaagaugag      600

aauuuagaaa ucauuuacaa cgacgagcug cuuacccaaa ucgagaauau cuuuaaacag      660

gauauuucua gaagcaagaa guuggaccag gccauagcuc uguuccaggg aguuaaaagg      720

caaucccuau uuggcaucuu ccucacacug auugugggca acaaggcaaa uuuccagaaa      780

aucuuuaacc uugaagauga cauaaagcug gauuugaaag aggaagacua ugaugagaau      840

cucgaagaac uguuaucaaa cauugacgag ggguaccggg acgucuuccu gcaggccaag      900

aacguguaua augcuaucga acucaguaag auccuuaaaa ccgauggaaa ggagacuaaa      960

gccccacuga gcgcgcagau gguagaauug uacaaccaac accgugagga ccuaaagaaa     1020

uauaaggauu acauuaaggc auaccugccg gaaaaauaug gugagacauu uaaggacgcc     1080

accaaaaaug gcuacgcugg guauaucgau ggaaagacgu cucaggagga cuucuacaaa     1140

uuuguuaagg cccagcucaa gggcgaagag aacggugaau auuuccugga ggcaauugag     1200

aaugaaaacu uucuucgcaa acaaagaucc uucuacaaug gggugauacc cuaucagauc     1260

caucugcagg aguugacugc uguccucgau cagcaagaaa agcacuacuc auuucugaaa     1320

gagaacaggg acaagauuau cagcuuacug accuuccgga ucccuuacua ugugggacca     1380

cuagccaaag gcgaaagucg auuugcgugg cucgagcgcu cuaauuccga ggaaaagauu     1440

aaacccugga acuucgauaa gauaguggac aucgauaaga gcgccgagcu guuuauugaa     1500

aaucuuacau cgagagacac uuacuugccu gaugagccag uccugcccaa aaggucucuc     1560

aucuaucaga aguucaccau uuucaacgaa cugacaaaaa ucuccuacau agacgagcgg     1620

ggcauccuuc agaauuuuuc aagucgcgag aagauugcaa ucuucaacga ccuguuuaaa     1680

aauaagagca agguuaccaa aaaccaguug gugaaguaua uugaaaacaa agagcaaauc     1740

auagcuccug aaauuaaggg gaucgaggau uccuucaauu cuaacuacag cacguauauu     1800

gaccucucaa aaaucccaga uaugaagaag cuguuagaaa aagacgagga ugagauacug     1860

gaagagauca uuaagauccu aacuauuuuu gaagacagaa aaaugaggaa gcgucagcuc     1920

augaaauuca aggauaagcu gagugagaaa gccaucaauc agcuuuccaa gaaacacuac     1980

acaggauggg gucaguugag cgagaagcug auaaacggca uucgggacga acaaucuaau     2040

aaaaccaucc ucgauuaccu gaucgaggac aacggguguc cgaagaauau gaaccgaaau     2100

uuuaugcagu uaauuaacga ugacacucug ucauucaaag aaaagaucag aaaggcucag     2160

gauauuaauc agguaaacga cauaaaagag aucgugaagg accuucccgg aaguccugcc     2220

auuaaaaagg gcaucuauca aagcauuagg aucgucgaug aaauaauccg caaaaugaag     2280

gaccggccaa agaauauugu gaucgagaug gcaagagaga accagacaac ccaggaaggu     2340

aaaaauaagu ccaaagccag guugaagaaa auucaagagg ggcucgaaaa ccuggauucu     2400

guucaugugg agaagcaggc ucuagacgaa gagaugcuga agucgcccaa auacuaucuc     2460

uacugccugc agaacggcaa ggauaucuau acgggaaaag accuugauau aggccaguug     2520

caaaccuacg acauugauca caucaucccu cgcagcuuua uuacagacaa uuccuucgau     2580

aaccuggucu uaacuucaag uaccgugaau cgagggaagc ucgacaacgu accaucuccc     2640

gacauaguuc ggcagcagaa aggauuuugg aagcagcugc uucgugcggg ucugaugagc     2700

caaagaaagu ucaauaacuu gacaaaaggc aagcucacug auagggaccg ccagcaguuu     2760

aucaaucggc agcuggugga gaccagacaa auuacaaaac augucgcaaa ccuacugucc     2820

caccaccuca augaaaagaa agaggugggg gaaaucaaca uuguccugcu uaagucagcc     2880

uugaccagcc aguucaggaa gaaauucgau uuuuauaagg ugcgagaggu uaaugacuac     2940

caucacgcuc augaugccua ccugaacgga gugaucgcau uaaaacuccu ggaacuuuau     3000

ccguacaugg ccaaggaccu gauauauggc aaauacucuu aucaccgcaa gaucgagggu     3060

gauaaagcua cucaggccaa guacaagaug uccaauauua ucgagcgguu cagucaagac     3120

cuacucgcaa acccugaugg ggaaauugcg ugggagaaag acaaggaucu gaauacgauc     3180

agaaaaguau ugagcucuaa gcagauaaac auuaucaaaa aggcugaaga gggcaaggga     3240

aggcuguuua aagagacaau caacucccgu cccucaaaga aaaccgaaaa gcgcauucca     3300

aucaaaaaua acuuagaccc uaauauauau ggcggguaca uugaggaaaa gauggccuac     3360

uauaucgcaa uuaacuaccu cgagaaugga aagacuaaaa aggccaucgu cgguauaagu     3420

auuaaagaua agaaagacuu cgaaggccag accacagagu aucuggggaa gaucggauuu     3480

aacaaggcua gcaucauuaa uucguucaaa aacuacaccc uuuuugagcu ggaaaauggc     3540

ucccggagaa ugaucguggg ugccucuaag gagaacgaca gcaaagggga auugcagaag     3600

ggcaaucaaa uguaucuccc ccagaaccug cuagaguucg uuuaccaucu gaaacacuau     3660

aaugaagaug agacuucaca caaguuuauu guggagcaua aagcauacuu cgacgaacuu     3720

cucaacuaca uagucgaguu ugcuaauaag uaucuggaau uggagaacag uaucgaaaag     3780

auuaaagauc uguaccacgg aaagggccca gacguggagg agaaagaauu aguagagucc     3840

uucaucaauc uccuggccau cacaaagugu gggcccgcgg ccgauauuac guuccuugga     3900

gagaaaauau cuaggaagcg auaucggagc accaacugcc uguggggcuc cgaagugauc     3960

uuucagucac cuacugguuu guacgagaca cgccucagac uggaauga                  4008


<210>  2964
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2964
auggacgaua acuaccagcu gaugcacgcc aagggcaaau auggguacgg aaccagaauc       60

uauagcgagg gucaaacagc ugcagacagg cggggcuucc gcacuacgcg acguagacuc      120

aauaggcgga aguggcgccu uagacuguug caggacuuuu ucgaacccua cauuuuaccu      180

caggaugagg ccuuuuucau aaggcagaaa caauccaacc uggugccaca ggauaccaag      240

aaacaguauc cggaaucugu ccuauuugac gauacaucag acaaggauuu cuacacugag      300

uaucccacca ucuaccaucu ccggcaggcg cugaugacag aaaaucgcca auuugacauu      360

cgagagguuu accuugcuau ccaccauauu gugaaguaua gagggcacuu ccugaacaau      420

gccccuguaa aaaccuuuaa gaguggaaac uuggaucucg caaucaaauu caaugagcug      480

aacgaagagu uuaagagcgu guacccagac cuggaacucc aguucaauuu agaucagaua      540

gacgagauca aaaagauucu gcuugacucc acuaagucua gguuugaucg gcagggcgag      600

gccgucaaau ugaucuauac agugucggaa guuaagacca uugagaaacu gcaaaacaag      660

aucgugaaac aguuccuauc agguauacuc gggcugaaua cgcguuucga cguccuuacu      720

aacaccgaaa gcgaugacag uauguggaag uuuucccugg auucugagag cuccgacgau      780

gcuuuggacg aaauuaucgc acagcucgau gaccccgcca uucauguggu agagcugguu      840

aaugagaucc acucaaguau cacauuagcu ggaauugugc cugaaggcca aggccugagc      900

gagucuaugg ucgaaaaaua cgaggcccac aaggcgcauc uccagcuucu gaaggcagug      960

gaaaaauugg uggauaagaa agccgcugac aagcuagagg ucgccuauga ccaguacaua     1020

aacgggaaag auuccaagca guucaucaag gaggacuuuu auaaagcagu ucaaucagaa     1080

auuaagggag uggcugguga ugagguagcc gaaaucaauc agcugauaga gcucgaccag     1140

uucaugccaa aacagcgcac uaaggaaaac ggcgcgauuc cccaccaacu gcaucagcac     1200

gagcuugaug ugaucaucga gaaccagagc aaauacuacg acuggcuggg ggaaaagguc     1260

ggaaaugagu auaaguugga uaaacucgug aguuuuagag uuccuuacua ugugggccca     1320

cuggucgaca ccgccucuaa ggagaaaaag gaugaaacaa gguucgcaug gauggugcgg     1380

aaaaacccgg aguccaccga agcuauuacg cccuggaauu uugaggacau cguagauaag     1440

aacgccagcg cugaaaauuu cauuaagcga augacuacaa aagacaagua cuuacugaac     1500

gaggauguuc uaccuaaaug cucgcuccug acccaggacu uugaagugua uaaugagcuu     1560

aacaauauac gcgucaacgg ugagaaguuc acugccgaac aaaaacaguu gauccacgac     1620

gagcuguuua caaagcaugc aucugugucc auuaaacagc ucaaggauuu ccuggucuca     1680

gaacuuaugu acgugagaga caaucugaag guugaggggu uggccgauga gaaaaagcuc     1740

augaguagcc uguccaccca guuugacuau cagaaaauca ucccagauca aauuaacgac     1800

gugaaguaca gggaagauau agagcagauc auugaauggu cuacgaucuu cgaggacgcu     1860

gcgauuuuca aaaccaaguu aaaggaaauc gauuggcuga cagacgagca acggaauaaa     1920

cuagcaaaca agcgcuacag aggauggggc agccucucaa aaaggcugcu ugccuguuug     1980

guagaugaaa auggccagcg uauaaucgac auucugcggg auaguuccga caacuuuaug     2040

augaucguca accgacccga guucaagcag gcuauuuuug acgagaaugc caaccucacu     2100

gaaaccaaua gcaaaaagga uauguucgag augaucgaca acuuuuauac aucuccucag     2160

aauaagaaag caauaagaca agugcuguua guugugcagg auauucagaa cgccauggga     2220

ggggucgcuc cacagaagau cuucauugaa uuugccaggg aggacgaggu gaauggucgc     2280

cggacuaacu caagauacag ucaaaucagg gaaauguaua gcaaaguaac cgaugacgug     2340

auaaauaaga caguucgcgg cgagcuggaa gcaaacaaag ggucccuuaa uaaggagaag     2400

uuguuccucu acuuucugca gggaggcauc gaucuauauu cugguaaaac cauuaacauc     2460

gacaauauuu cgagcuacga ugucgaccac gugcugcccc aguccuucau caaggaugac     2520

ucaauaaaca auaaagugcu cgucaacgcg aaggggaacc gaguuaaaaa ugacaacgug     2580

ccgauggaac uguuuggcgc uaagauguau ccuacuugga aacggauggu ggauacggga     2640

auuaugagua aggccaaguu caaaaaucuu acauugaacc ccgacaaugu agauaaguuu     2700

aaagcagagg gcuucaucaa ccgucagcug gucgagacca gacaaaucau uaaguuagug     2760

gcccagaucc ucaaugacaa acugggggau gacacuaaga uuguuucugu gaaggcuaac     2820

cuuacccaug auaugagggu cuucuuugac uucccuaaaa aucgcaacgu gaauaacuac     2880

caccaugccu uugaugcaua ccugagcgcu uucguuggaa cauauuugua caagcgguau     2940

ccaaaacucc agcccuacuu uguguauggu gaguucaaga aaauggacaa gcugaagaau     3000

augaccuccu uugauuuccu aagagaccug aaacacacug auaagguagu cgacaaagac     3060

acaggcgaaa uaaucgccga uaagcucgcg gugauuacga acaugaauaa aaucuacgac     3120

uuuaagaagg ugcuggucac ccacgaaguu caugagaacu caggggccau guaugaucaa     3180

acucuuuacc cagcuaaauu ggacaagagc cugggaagga aaggcgcaaa gcaguuaauu     3240

cccaaaaagg augacaugcc uacagcccuc uacggugggu auaccggcuc uaaggaugcu     3300

uucaugucca ucgugcgacu gaaaaagaaa gacgaaguau acuauaaggu gauaggaauc     3360

ccaguccgca uugccgauag ugugcagcac ggcaaagagg uugacgugga ggcacuuaca     3420

caguuuaucg aaacuaaguu caccaaagag aaccugaaua agaagacggg ggaaauuacc     3480

cggaaagucg agugguucga gcuagugcuc ccgaagguuc uguuuggaca agcgguguuc     3540

gaugguggcc agcccuuuau guuggggagc ucuacauacc cucauaaccu gagagaauua     3600

guccucacua aagacuccgu aaagaaaauc gccggaaagg agucaaauaa gaguuaucug     3660

gaugcuuacg acgaaauaau ggaggccaug aacaaauauu ucccacuuua cgacaccaau     3720

aaguuuaggg aaaaagugau ugagggccgu agcaaguuca ucgaucugcc cgacaaagau     3780

gaguacgaaa acaauaaguu gguucaggug gguaagucga caaucauuga ccgcauguuu     3840

auagggcucc acgcaaacgc ugcccggacu gaucugaaaa auaucggcau uaccacgccu     3900

uucggauuua ugcagcaagc auccggcauc aagcuaacaa aagacaccgu cgugcuguuc     3960

cagucuagca cugggcuuuu ugagagaaag guagcccuca aagaucugug a              4011


<210>  2965
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2965
augaccaagg acuacacaau cggccuggau auugggacua acagcguggg augggccguc       60

cucacggacg auuaucagcu uaugaaaaga aagauguccg uucacgguaa uaccgagaaa      120

aagaaaauaa agaagaacuu cuggggcgcu aggcuguuug acgaagggca aacagcagag      180

uuccggcgca cuaaacgaac caaucguaga agguuggccc ggcgcaagua cagauuaucu      240

aaacugcagg aucuauuugc ggaggaacuc ugcaagcagg acgauuguuu cuuugugagg      300

cuggaagagu cauuccuugu acccgaggaa aaacaguaua agccugcuag uaucuuucca      360

acacuggagg aagagaagga guacuaucaa aaauacccga ccauuuauca uuugcggcag      420

aagcucgugg acagcacuga aaaagaggau cugcgccugg ucuaccucgc cuuagcacau      480

cugcuuaagu accgaggaca cuucuuguuu gaaggcuauc uggacacaga gaacaccucc      540

aucgaagagu cuuucagagu guuucuagag caguacggua aacagucgga ucaaccccuc      600

auuguucacc agccugugcu gacgauccuu acugacaagc ugucaaaaac caagaagguc      660

gaagagauau ugaaauauua cccaacagaa aagaucaaua gcuucuuugc ccagugccuc      720

aaacugauug uggggaacca ggcuaauuuc aagaggaucu ucgauuuaga ggcagaagua      780

aaacugcaau uuaguaagga gacuuaugag gaagaccucg agucccuucu ggaaaagauu      840

ggagacgagu acuuggauau cuuccuacag gccaaaaagg uucaugacgc uauacugcuc      900

ucugaaauua ucagcuccac cgugaaacac acacaggcca agcugucaag uggcaugguc      960

gagcgguaug agcgucacaa agcggaucuu gcaaaguuua agcaauucgu gaaagaaaac     1020

gugccccaga aggccaccgu cuuuuucaaa gacacgacua agaauggcua cgcuggguac     1080

auuaaaggaa agacaaccca ggaggaauuu uauaaguucg uuaaaaagga gcugagcggu     1140

gugguaggcu cugagccauu uuuggagaaa aucgaucagg aaacuuuccu ccugaagcaa     1200

cgcacauaca ccaacggggu gaucccucau cagguccacu uaauugaacu gaaagccaua     1260

aucgaccagc agaagcaaca uuaucccuuu cuagaggaag caggaccuaa aauuaucgcu     1320

cucuucaagu uuagaauacc auacuaugug ggcccgcugg ccaaggagca ggaggcgucc     1380

ucauucgccu ggauugaaag gaaaacggca gagaagaucc accccuggaa uuucagcgaa     1440

guuguggaua ucgagaaaag ugcuaugcgg uuuauucagc gaaugaccaa gcaggacaca     1500

uaccuuccua cugaaaaagu cuugccaaag aacucucugc ucuaucaaaa guacaugauc     1560

uucaaugagc ugaccaaagu guccuacaag gaugagcgcg guguaaaaca guauuuuagc     1620

ggggacgaaa agcagcagau uuucaaacaa cuuuuucaga aggagagagg aaagauaaca     1680

guuaaaaagc ugcagaacuu cuuguacacu cacuaucaua ucgaaaaugc ccacauuuuu     1740

ggcaucgaga aggcuuucaa cgccucguac ucuaccuauc augauuuuau gaagcucgca     1800

aaaacaaacc aaaaguccau gcaggaaugg cuggagcagc ccgagaugga accuaucuuc     1860

gaggacauug ugaagauauu aaccaucuuu gaagacaggc agaugauuaa acaccaacug     1920

ucaaaguacc aggaggucuu cggcgagaaa cuacucaagg aauuugcccg gaaacauuau     1980

acuggguggg gacgcuucag ugcuaagcug auccacggua uuagagauag gaaaacgaau     2040

aagacaaucc uugacuacuu gauaaacgau gacgaugugc cagcgaaucg uaaccggaau     2100

cugaugcagc ucaucaacga cgagcaccug agcuucaagg aagagauugc aaaagccacc     2160

gucuuuucca agcauaaauc uuuaguggau guuauccagg accugcccgg cagcccggcu     2220

auuaagaaag ggaucuggca aucacuuaag auaguggaag aguugauugc caucauugga     2280

uacaagccua aaaauaucgu aauagagaug gcacgagaaa accagaagac ucacagaacc     2340

aaacccaggc ucaaggcccu ggagaauggc cuaaaacaga ucgguaguac acugcucaag     2400

gaacagccaa ccgauaacaa ggcucugcaa aaagagcgcc uuuauuugua cuaucugcag     2460

aaugggcggg acauuuacac uggcgaaccu uuagauaucg agaaccucca ucaguaugag     2520

gucgaccaca uuauccccag auccuucaua guggauaaua gcauugacaa caagguucug     2580

guggccucua aacaaaauca gaagaaaagg gacgaugucc caaacaagca gaucgugaac     2640

gaacagcgca ucuuuuggaa ucaacuuaag gaggcaaaac ugauuucacc caagaaauac     2700

gcguauuuga caaagaucga acucacgccu gaggacaaag cucgauucau ucagcggcag     2760

cugguagaaa cccgucagau aacuaagcac guggccaaca uccuacauca aaguuuucac     2820

caggaggagg aaggaacaga uugugacggc guucagauua ucacccugaa ggcaacacuc     2880

acuagccagu ucagacaaac cuuugggcug uacaaaguca gggagauuaa uccacaucac     2940

caugcccacg augcuuaccu uaacggauuc aucgccaaug uguugcugaa gcgcuauccg     3000

aaauuagcac ccgaauuugu guacgguaag uaugucaaau acucccucgc ucgggagaac     3060

aaggccacgg cgaaaaagga guucuauucu aauauacuga aguuucuuga aucggacgag     3120

ccuuucugcg augaaaacgg cgagaucuac ugggagaaaa gcuaucaucu gccaagaauu     3180

aagaaaguuc uauccucaca ccaggugaau gugguaaaga aagucgaaca gcagaaaggg     3240

ggauucuaca aggagaccgu gaacaguaaa gaaaagcccg acaaacucau cgagaggaag     3300

aauaacuggg auguuacaaa auacggcggu uuugggucuc cugugauugc cuaugcuauc     3360

gcauucgucu acgccaaggg caagacucag aaaaagaccc gagcuauaga aggaauuaca     3420

aucauggagc aagccgcauu ugaaaaagac ccaacuaccu uccugaagga uaaaggcuuu     3480

ccccagguga cggaguucau caaguugccg aaguauacac uguuugaguu cgacaauggg     3540

cgccggagau uuuuagcgag ccacaaagaa ucccaaaagg gaaacccuuu cauucucuca     3600

gaucagcugg uuacccuucu guaccaugcc cagcacuaug acaaaauaac uuaccaggag     3660

agcuuugacu augugaauac acauuugucu gauuucuccg cuauccucac cgaaguacug     3720

gccuuugcag agaaguacac ucuagcugac aaaaacauug aaaggaucca agagcuguac     3780

gaggaaaaca aguaugguga gauuaguaug aucgcccaga gcuuccuuca gcuccugcag     3840

uucaaugcaa uaggcgcccc agcugauuuu aaauucuuug gggucaccau cccccguaag     3900

cgcuacacau cuuugacgga aauuugggac gcgaccauca uuuaucaauc cgugacugga     3960

cuguacgaga cacggaucag aaugggcgau uuaugggccg gugagcagug a              4011


<210>  2966
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2966
augaccaagg acuacacaau cggccuggau auugggacua acagcguggg augggccguc       60

cucacggacg auuaucagcu uaugaaaaga aagauguccg uucacgguaa uaccgagaaa      120

aagaaaauaa agaagaacuu cuggggcgcu aggcuguuug acgaagggca aacagcagag      180

uuccggcgca cuaaacgaac caaucguaga agguuggccc ggcgcaagua cagauuaucu      240

aaacugcagg aucuauuugc ggaggaacuc ugcaagcagg acgauuguuu cuuugugagg      300

cuggaagagu cauuccuugu acccgaggaa aaacaguaua agccugcuag uaucuuucca      360

acacuggagg aagagaagga guacuaucaa aaauacccga ccauuuauca uuugcggcag      420

aagcucgugg acagcacuga aaaagaggau cugcgccugg ucuaccucgc cuuagcacau      480

cugcuuaagu accgaggaca cuucuuguuu gaaggcgacc uggauacaga gaacaccucc      540

aucgaagagu cuuucagagu guuucuagag caguauucga aacagucaga ccaaccccuc      600

auuguucacc agccugugcu gacgauccuu acugauaagc ugagcaaaac caagaagguc      660

gaagagauau ugaaauacua uccaacagaa aagaucaaua guuucuuugc ccagugccuc      720

aaacugauug uggguaacca ggcuaauuuc aagaggaucu ucgacuuaga ggcagaagua      780

aaacugcaau uuuccaagga gacuuacgag gaagaccucg agucucuucu ggaaaagauu      840

ggggaugagu auuuggacau cuuccuacag gccaaaaagg uucaugaugc uauacugcuc      900

agcgaaauua ucuccucaac cgugaaacac acaaaggcca aacugaguag cggaaugguc      960

gagcgguacg agcgucacaa ggcggaccuu gcaaaguuua aacaguucgu gaaggaaaac     1020

gugccccaaa aagccaccgu cuuuuucaag gauacgacua aaaauggcua ugcuggcuac     1080

auuaagggga agacaaccca ggaggaauuu uacaaauucg uuaagaaaga gcugucugga     1140

gugguagguu ccgagccuuu cuuggaaaag aucgaccagg agacuuuccu ccugaaacag     1200

cgcacauaua ccaacggcgu gaucccacau caaguccacu uaauugaacu gaaggccaua     1260

aucgaucagc agaaacagca uuaccccuuu cuagaggaag cagggccuaa gauuaucgcu     1320

cucuucaagu uuagaauacc auauuacgug ggaccgcugg ccaaagagca agaggcgagc     1380

agcuucgccu ggauugaaag gaagacggca gagaaaauca aucccuggaa cuucagugaa     1440

guuguggaca ucgagaaguc ugcuaugcgg uuuauucagc gaaugaccaa acaggauaca     1500

uaucuuccua cugaaaaggu cuugccaaag aauagccugc ucuaccagaa auauaugauc     1560

uucaacgagc ugaccaaggu gagcuacaaa gacgagcgcg gcguaaagca auacuuuucg     1620

ggugaugaaa aacagcagau uuucaagcag cuuuuucaaa aggagagagg gaaaauaaca     1680

guuaagaaac ugcagaauuu cuuguauacu cacuaccaca ucgaaaacgc ccagauuuuu     1740

ggaaucgaga aggcuuucaa cgccucuuau uccaccuacc augacuuuau gaaacucgca     1800

aagacaaauc aaaaggccau gcaggaaugg cuggagcagc ccgagaugga accuaucuuc     1860

gaggacauug ugaaaauauu aaccaucuuu gaagauaggc agaugauuaa gcaccaacug     1920

ucaaaauauc aggaggucuu cggcgagaag cuacucaaag aauuugcucg gaagcauuac     1980

acuggcuggg ggcgcuucag ugcgaaacug auccacggaa uuagagacag gaagacgaac     2040

aagacaaucc uugauuauuu gauaaaugac gaugacgugc cagcaaaccg uaaucggaac     2100

cugaugcagc ucaucaauga ugagcaucug agcuucaaag aagagauugc caaggcuacc     2160

gucuuuucca aacacaaguc uuuaguggac guuauccagg aucugcccgg uagcccggcc     2220

auuaaaaagg gcaucuggca aucacuuaag auaguggaag aguugauugc aaucauuggg     2280

uacaaaccua agaacaucgu aauagagaug gcccgagaaa aucagaaaac ucacagaacc     2340

agucccaggc ucaaggcucu ggagaacgga cuaaaacaga ucggcuccac acugcucaag     2400

gaacagccaa ccgacaauaa ggcccugcaa aaagagcgcc uuuacuugua uuaccugcag     2460

aacggucggg auauguauac uggggaaccu uuagagauug agaaucucca ucaguacgaa     2520

gucgaccaca ucauucccag aucauucauc guggauaacu cuauagacaa uaagguucug     2580

guggcaucaa aacaaaacca gaagaaaagg gacgaugucc caaagaagca gauugugaac     2640

gagcagcgca ucuuuuggaa ucaacuuaaa gaagcgaagc ugaucagucc caaaaaguau     2700

gcuuacuuga caaaaauuga gcucacgccu gaagacaagg cccgauucau ccagcggcag     2760

cugguagaga cccgucagau uacuaagcau guggcaaaca uacuacacca auccuuuaau     2820

caggaggaag agggcacaga uugugacgga guucagauca uuacccugaa agccacacuc     2880

acuucccagu ucagacaaac cuuuggccug uauaagguca gggaaaucaa cccacaccau     2940

cacgcucaug augccuaccu uaauggguuc auugcaaacg uguugcugaa acgcuacccg     3000

aaguuagcuc ccgaguuugu guauggaaaa uacgucaagu auucucucgc ccgggagaau     3060

aaagcgacgg ccaagaagga guucuacucg aacauccuga aauuucuuga aagcgacgaa     3120

ccuuucugcg augagaaugg ugaaauauau ugggagaagu cccaccaucu gccaagaauc     3180

aaaaagguuc uaucaaguca ccaggugaac guggugaaaa aggucgagca gcagaagggc     3240

ggguucuaca aagaaaccgu aaauucuaag gagaaacccg acaagcucau ugaaaggaaa     3300

aacaauuggg auguuacaaa guauggaggc uuugguagcc cugugaucgc uuacgcaauu     3360

gccuucgucu acgcuaaggg gaaaacucaa aagaaaaccc gagccaucga gggcauaaca     3420

auuauggaac aggcagcguu ugagaaggac ccaacuaccu uccugaaaga gaagggauuu     3480

ccccagguga cggaauuuau caaguugccg aaauauacac uguuugaguu cgauaacggc     3540

cgccggagau uuuuagccuc ccacaaggaa ucacaaaaag ggaauccuuu cauccucagc     3600

gaccagcugg uuacccuucu guaccaugcu cagcacuaug auaagauuac uuaccaggag     3660

ucuuuugacu augugaacac acauuugucc gacuucagug ccauacucac cgaaguacug     3720

gcauuugcug agaaauacac ucuagccgau aagaacaucg agaggauuca agaacuguau     3780

gaggaaaaua aauacggaga gaccagcaug aucgcacagu cuuuccuuca gcuccugcag     3840

uucaacgcca uuggugcucc agcggacuuu aaguucuuug gcgucacaau cccccguaag     3900

cgcuacacgu ccuugaccga gauaugggau gccacuauca uuuaucaauc agugacaggg     3960

cuguacgaaa cccggaucag aaugggagac uuaugggcag gcgagcagug a              4011


<210>  2967
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2967
augaagaaca ugaaaaagcc cuacaccauc ggccuggaca uugggacaaa uagcguggga       60

ugggccgucc ucacugauca guaugaccuu guuaaaagaa agaugaaaau agcuggugau      120

uccgagaaga agcaaaucaa aaagaacuuc uggggcguga ggcuguuuga cgaagggcag      180

acggcagccg aucggcgcau ggcgcgaacc gcucguagaa ggauugagcg gcgcagaaau      240

aggaucucuu acuugcaggg aauuuucgcc gaagagaugu caaaaacaga cgcaaacuuu      300

uucugccggu uaagugauag cuuuuaugua gacaaugaaa agcgcaacuc ccgacacccu      360

uucuuugcca cuaucgagga ggaaguggag uaccauaaaa auuauccaac cauauaccac      420

cugagagagg agcuagucaa cucuucggag aaggcugauc ucaggcuggu guaucuugca      480

cuggcccaua ucauuaagua ccggggcaau uucuugaucg aaggugcucu cgacacacag      540

aacaccucag uugaugggau uuacaaacaa uuuauccaga cuuauaauca gguguucgcc      600

agcggaauag aggacggcag ucugaagaaa cuggaagaua acaaggacgu cgcgaaaauu      660

cucguggaga agguaacacg uaaagaaaag uuagagcgca uccugaaacu uuacccgggc      720

gagaaauccg cagggauguu ugcccaguuc auuucuuuga ucguuggaag caaggguaau      780

uuucaaaaac ccuucgaccu gaucgaaaag uccgauauug agugugcuaa agacucauau      840

gaagaggauc uagaaagucu ccuggcccuu auaggcgacg aguacgcaga gcuguucgug      900

gcugccaaga acgcguauag cgccgucgug uugucuucca ucauuaccgu ggcagaaacg      960

gagacuaaug cuaagcucuc agccagcaug aucgaaagau uugauaccca cgaggaagac     1020

cugggggagu uaaaggcuuu cauaaagcug caucucccua aacacuacga ggaaauuuuu     1080

aguaacacag agaagcacgg auaugccggc uacaucgaug guaaaacuaa gcaggcagac     1140

uucuacaagu auaugaaaau gacccuugaa aacaucgagg gggccgauua cuuuauugcu     1200

aagaucgaga aagaaaauuu ccugaggaag cagcggacau uugacaacgg agcgauucca     1260

caucaguugc accuagagga acuggaggca auacuccauc aacaggccaa auauuacccc     1320

uuccugaagg aaaauuauga uaaaaucaag ucucuuguca ccuuucgcau uccuuacuuc     1380

guuggcccac uggcuaacgg ccaguccgaa uuugccuggu ugacgcgaaa ggcagacggg     1440

gagaucagac ccuggaauau cgaagagaaa guggacuucg gaaagagcgc cguagauuuu     1500

auugagaaaa ugacuaacaa ggacacauau cucccuaaag aaaaugugcu gccaaagcac     1560

ucguuaugcu accaaaagua ccuggucuau aacgaacuaa ccaaagugag guacauaaau     1620

gaucagggua agacuucuua uuucuccggc caggagaaag agcagaucuu caacgaccuc     1680

uuuaagcaaa aacggaaggu uaagaaaaag gaucuggaac uuuucuugcg caauauguca     1740

cacguggaga guccgacaau ugaagggcug gaggacagcu uuaacuccuc uuacagcacc     1800

uaucaugauc uccugaaagu cggaaucaag caggaaauuc uugacaaucc cgugaacacg     1860

gagaugcugg agaauaucgu aaaaauauug accguuuucg aagauaagag aaugaucaag     1920

gagcagcucc agcaauuuuc agacgugcug gauggcgucg ugcugaaaaa guuagaaagg     1980

cgucacuaca cagguugggg gcggcuaagu gcuaaacucc ugaugggcau ucgagacaag     2040

cagucccauc uuacuaucuu ggauuaucug augaacgacg acggacucaa cagaaaucug     2100

augcaguuaa uuaacgauag caaucugucu uucaaaucaa ucauagagaa ggagcagguc     2160

accacagccg acaaggauau ucaaaguauc guggcagacc uugcgggcag cccugcuauu     2220

aaaaagggga ucuugcaguc ccucaaaaua guugaugaac uggugucugu aaugggauac     2280

ccaccccaga cuaucgucgu ggagauggcc agggaaaacc agaccacagg uaagggcaaa     2340

aauaacucgc gcccucggua caagagccua gagaaagcaa uuaaggaauu ugggucccaa     2400

auccugaagg agcacccaac cgacaaucag gagcucagaa acaauaggcu guaucuuuac     2460

uauuugcaga acggaaaaga uauguacacu ggccaagacc uggauauuca uaauuuauca     2520

aacuaugaca ucgaucacau aguuccccag aguuucauua cggacaauuc uaucgauaac     2580

cucgugcuga caagcuccgc cgguaaucgc gaaaaggggg acgacguccc gccucuugag     2640

aucgugcgaa aacggaaggu auuuugggaa aaacuguacc agggcaacuu gaugucaaag     2700

cguaaguucg auuaucucac caaagcugag agaggaggcc ugacugaagc cgacaaggca     2760

agguuuauuc accgccagcu aguggagacc cggcaaauca ccaaaaacgu ugcuaauauu     2820

cugcaucaga gauucaacua cgagaaggau gaccacggga auacaaugaa acaggucagg     2880

auagugacuc ucaagagcgc ccuggugucu caguuucgaa agcaauucca gcuuuacaaa     2940

guccgcgaug uuaacgacua ucaucacgcg cacgaugccu acuugaaugg agugguggcu     3000

aacacacugu uaaagguaua uccccagcuc gaaccagagu ucgucuacgg ugacuaucau     3060

caguuugauu gguucaaagc aaauaaggcc acggcuaaaa agcaauuuua caccaacauc     3120

augcuguucu uugcccagaa ggaccggauu aucgaugaaa auggcgagau ucuuugggac     3180

aaaaaguauc uggacacugu gaaaaagguu auguccuaca gacagaugaa caucgugaaa     3240

aagacagaaa uacaaaaggg ggaguucagu aaagcaacca ucaagccuaa aggaaauagc     3300

ucuaagcuaa uuuccaggaa aacaaacugg gaucccauga aguacggcgg ucucgacuca     3360

ccaaauaugg cguaugccgu cgugaucgag uacgcuaaag ggaagaacaa gcugguuuuu     3420

gagaaaaaga uuauccgugu gacuauaaug gaacgcaaag ccuucgaaaa ggaugagaaa     3480

gcauuuuugg aggaacaggg cuaucggcag cccaagguac uggcuaaguu accuaaauac     3540

acccucuaug agugugaaga gggaagaagg cgaaugcugg ccagugcaaa ugaagcccag     3600

aagggcaacc aacagguccu uccaaaccac cuggugacgu ugcuccauca cguggcuaau     3660

ugcgagguca gcgacgggaa aucgcuggau uacauugagu ccaaccggga aauguucgcg     3720

gagcuacugg cccauguuuc ugaauuugca aagcgcuaua cccuugcuga ggccaaucuc     3780

aacaaaauca aucagcuguu cgaacagaac aaggagggag acaucaaggc cauugcucag     3840

agcuucgugg auuugauggc auuuaaugcc augggcgcgc cggcuucauu caaauuuuuc     3900

gagacaacua uagaaagaaa gagguacaac aaucugaaag aguuacucaa caguaccauc     3960

auuuaccaau ccaucacagg ucuguaugaa ucucggaagc gacuugacga uuga           4014


<210>  2968
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2968
augaagaaau ucgagaacua cuaucugggc cucgacaucg ggaccagcuc cauuggaugg       60

gccgugacaa auucucagua cgauauacuu aaguuuaacg guaaauauau guggggcacu      120

agacuguucc ccgaagcuaa uacggcacaa gagaggcgga uccaccgcuc aagucgacgu      180

agauugaaga ggcggaaaga acgcauucag aucuuacaga ugcuguuuga caaggagauu      240

gccaagaucg auagcggguu cuuucagaga cuaaaagacu ccaaguacua uaaagaagau      300

aagaccgaga aacaaacaaa cucuauauuc caugacaagg auuacucgga caaggaguau      360

caccaggauu uuccuacuau cuaccaucuc aggaaauucc ugcuugaagg aaauaagcca      420

aaagacauuc gguuugucua ccuggcguug caccauaucc ucacccaccg cggccacuuc      480

cuguuuccgg auauggaggu uucaaacgug acagaguuca gcaauauuuu uagugaacug      540

aagcaguauc ucuacgacga gauggauuua gacuucgaau ggaaaaacgg uaagcugaau      600

gagguagaaa acauccuuaa agaccgaaau uuggcuaagu ccgagaagga gaaaaagcug      660

ugcgggauaa uugaacuaga gggaaaaauc gauaaccaga gaaaggccau gauuggcaug      720

auguguggcu gcaaaaagaa guucucugac cucuuucaaa augaaaacua uagggagcug      780

gaaaucagcu ccuucucauu ugcagauagu agcuacgagg aaaaacggga cgagcuugag      840

gggauccugg ccgaaaauau uuugugucuc gauuaucuga agaccauaua cgacugggga      900

aaauuaucug auauccugca gggugaggaa uccauuucag uggcuaaggu caaaagcuau      960

gaggaacauc agcucgaccu uaaguaccug aagaacaucu ugaaacuaua cagucacaag     1020

gagaaagaga acguguuccg uaagaaagaa ggcaaguauc ccaaguacau aucugggaaa     1080

cugucccagg aggaauuuaa uaagagcauu aaaaagaucc ucgaggagau caaaucgguu     1140

aagcaagaag aucgcaaagu guucgacacu cugcuuaaga gagcaucuaa caaucuguug     1200

ugcccuaagc aggucauuaa agagaacgga gugaucccau aucagauuca uaaguuugaa     1260

cucgagaaaa uacugaggaa uauggccgau uucuuuccca uguuaaagga aaagaaagac     1320

ggcaagacac uguccgagaa aaucauuuca guauucaccu uucggauccc uuacuauguu     1380

gguccacuaa accaaaauag ugaccgagcu uggcucguga agaacaaaga ugagaagauc     1440

uaccccugga auuucgaaga gauugucaac cuggaagaga gcgccgaaaa auuuauacag     1500

aaucuuacga acaaguguac uuauuuggug cuggaggacg ugcucccuaa guccucuauc     1560

cuguacagca aauucauggu ccuuaaugag cugaacaauu ugaagauuga uggggaagcg     1620

aucucaguug accucaaaca gaagauuuac cugaacuuau uccagaaaua uaagaaggug     1680

acccugaaaa agcuaaaagg auaccucaag agugagaaca uccugauaga uacauccacu     1740

caaaucaccg gcauugacgg cgauuuuaaa agcucucuug ggucauauuu ggacuucuac     1800

aauauccugg gagauaaggu aaaaacagac uuugguaaga agcucauuga aaacugcauc     1860

cuguggauaa ccuuauauac gggcgagaaa aagcugcuua aaaauaagau uaucgcaaac     1920

uacaaagggg aauugaguga ggaggaaauu aagaagaucg ugaaucucaa auauaaggau     1980

uggggacgcc ugagcuacgc cuuccuagag gaaauacagu ccgcuucucu ggagacuggc     2040

gagcucagaa acaucauuca gaugaugugg gaaacaaaua acaaucugau ggagcuuuug     2100

ucgagcaacu accaguuucu guccgaaauc gagaaaagga acucagucgu ggccauuggu     2160

aaggaguuca auuaugaaac caucuuaggg gacaguuacg caucuccaag cguuaaacgg     2220

augauauggc aaucccucuc aguggucgau gagauuaaga agaucaugaa aaaggcuccg     2280

aaaaagaucu uuauugaaau ggcccgccag gaggacauga aaaaggaaag aaaggagagc     2340

aggaaaucua cuuuccugac acuuuauaag uccaucaaag aagagggccg ugacuggauu     2400

aaggagauag aaaauuggag ugauagcgag uuucggucua aaaagcugua cuuguauuac     2460

acccagaugg gaaaguguau guauacgggc gagaaaaucu cccucgacca gcuguucaac     2520

aagaauauuu acgauaucga ccacauuuac ccccgaucaa aaaccaagga ugacaguauc     2580

gaaaacauag ugcuaguaaa aagaaacauc aaugcgaaga aaacagauga auauccucug     2640

gagaggaaca uucaacagaa gcagcacgac uuuuggaaga ugcuccauag caaaaagcug     2700

aucggggaua aaaaguacga gcgccuuacu cggaccacag aguucacuga cgaagaauug     2760

ucggauuuua uugccagaca gcugguugag accaggcaau ccacaaaaau cguggcagac     2820

auauuaaaga aucucuuccc agagaccaag auugucuaug ugaaagcuaa ccugacuucu     2880

gauuuccgca agaauuuuaa aauccuuaag agccgagaca ucaacgacua ccaccaugcc     2940

cacgaugcuu aucugaauau ugucacggga aacguguaca auauaaaauu cacagacaac     3000

ccccggaauu uuaucaagga uaagaaacua gaagguaaga aauauaaccu caagguugag     3060

aaaauuuucg aauaccguaa ggagaaugaa ucauuuugga acccuaagac cauggugacu     3120

aaaaucgaga auuacauuuu caagaaaaga ccacaguuua ccagguauag uuucgagcag     3180

cauggcgggu uuuucgacca aaacaucgua gauaaggaau ccugcaaaaa gggaacaggc     3240

uacauaccca ucaaaucuag caacgccauu cuguauaaua ccuccaagua cgguggguau     3300

ggcucaguca cuggaacaua cuuuuucuug guggagcaca cgaugaaggg caaacgcauc     3360

cggaccauug aaaugaugcc gcuguauuua agcaagaaaa uccacaguaa ggaggaacuc     3420

gagaaauacu guaaggagaa gcuggaacuu cagaaaccuu cuguuagacu ggcaaggaua     3480

aaguacaacu ccuugcucaa aauuaauggg uuccccuauc auaucacugg aaagacaaac     3540

gacagcuacu ggaucauguc ggccauucag cugcuacugu cuaaaaauua uuacgaguau     3600

cuucgaaagc ucuacaucuu uugcaaggag gaacgccugg aagaggagau agugggugag     3660

aaaaacauua aguuguauac cuguauccug gaaaaauuag aaaagucaau uuacagucac     3720

aaacuccuga auaucaacua caauggcaag uccaagaacc uuaaagauau acuggagagc     3780

gagaaggaaa auuucuugcu ccugucugag aaaaagcagg cucaaauucu aaucgaaauc     3840

uuuacacugc uugagagcaa caauuucggg gcgaaccucg aauccuuugg augcggcaaa     3900

aagugcggua uuacuaaaau caauaagaac auugacaagc uggaggaggu cuuguuaaua     3960

aaucagucac caaccgggcu guucgaaaac aguaucgauc ucaaaaaggu guga           4014


<210>  2969
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2969
augaagaaag acuacgugau cggccuggau auugggacca acagcgucgg augggccguu       60

augacagagg acuaucagcu cgugaagaaa aagaugccca uauacgguaa uacugagaaa      120

aagaagauca aaaagaacuu cuggggcgua agacuuuuug aggaagggca cacggcugag      180

gauaggcggc ugaaacgcac cgcacgacgu agaauuucca ggcggcgcaa uagauugagg      240

uauuuacaag ccuucuuuga agaggcgaug acagaccugg augagaacuu cuuugcucgg      300

cuacaggaau cuuuccucgu gccugaggac aagaaauggc aucgccaccc aaucuuugcc      360

aagcuggaag augaggucgc auaccaugag acuuauccga ccauuuacca ccuucgaaag      420

aaacuggccg acucaaguga acaggcugau uugagacuca ucuaucuggc acuggcccau      480

auagugaagu acaggggaca cuuccucauc gagggcaaau uaagcacaga aaauauuucc      540

guuaaggagc aguuucaaca guucaugauc auuuacaacc agaccuuugu gaauggugaa      600

ucucggcugg ucucggcucc ccuuccugag ucaguguuga ucgaggaaga acugacugaa      660

aaagccagcc guacaaagaa aagugagaag guacuacagc aauucccaca ggaaaaggcg      720

aacgggcucu uuggacaguu ccugaaacuu augguuggca auaaggcaga cuucaaaaag      780

guguuuggcc uggaggagga agccaaaaua accuaugcuu ccgagucuua cgaagaggau      840

uuggaaggga uucucgccaa ggucggagac gaguauagcg auguguuccu ggcagcuaag      900

aacguguacg acgccgucga guuauccacg auccuggcgg acucagauaa aaagagucac      960

gccaaacucu cuagcuccau gauuguucgc uuuacugaac aucaggagga ccuuaagaaa     1020

uucaagagau uuaucaggga aaauugcccc gaugaguaug acaaccuguu caagaaugag     1080

caaaaagaug guuacgcagg cuauaucgcu cacgccggga aggugucaca guugaaauuu     1140

uaccaguacg uaaagaaaau uauacaagac aucgcuggag ccgaauauuu ccuagagaag     1200

auugcacagg aaaacuuucu gcggaagcag cgaaccuucg auaauggcgu gaucccucau     1260

cagauacacc ucgccgagcu gcaagcuauu auccaccgcc aggcggcaua cuauccauuu     1320

cuuaaagaaa accaggagaa gaucgagcag cuggucacau ucaggauucc cuacuaugug     1380

gguccuuuga gcaaagggga cgccaguacu uuugcuuggc ucaagagaca aucugaggaa     1440

ccaauccggc cguggaaccu gcaggaaacc guugauuuag accaguccgc cacagcauuc     1500

auugagcgca ugaccaauuu cgauacguac cugcccagcg aaaaagugcu accuaagcau     1560

ucgcuccugu augagaaauu uauggucuuc aacgagcuua cuaagauauc uuacacagac     1620

gacagaggaa ucaaggccaa uuuuuccggc aaagaaaagg agaaaauuuu cgauuacuug     1680

uuuaagacca ggcguaaagu gaagaagaaa gacaucaucc aguucuaucg gaacgaauac     1740

aauacugaga uuguaacacu gucaggccuc gaagaggauc aauuuaacgc uaguuucagc     1800

accuaucagg accugcuuaa gugugggcug acgcgagccg aguuggauca cccagacaau     1860

gcagaaaaac ucgaggauau aaucaagauu cugaccaucu uugaagacag acagaggauu     1920

cgcacacagu uauccacuuu caaaggacaa uuuucugcgg agguucugaa gaagcuagag     1980

cggaaacauu acaccgguug gggcagacuc agcaagaaac ugaucaacgg gauauaugau     2040

aaggaaucag gaaaaacaau ccuuggcuac uugauuaagg acgauggugu gaguaagcac     2100

uauaauagga acuucaugca gcugaucaau gacucccagc ucagcuuuaa aaacgcuauu     2160

caaaaggccc agucuucaga gcaugaagag acucugagug aaaccgucaa ugaguuagca     2220

gggagccccg ccaucaaaaa gggcauauac cagucccuga aaauugugga ugagcuuguc     2280

gcuaucaugg gauacgcccc uaagcgcauu gugguugaaa uggcacgaga gaaccagaca     2340

accucuacug gcaaacggcg uucgauccaa agauugaaga uaguggaaaa ggcuauggcc     2400

gagaucggga gcaaucuccu gaaagaacag ccaacgacaa acgagcagcu aagggacacc     2460

cgccuguucc ucuauuacau gcagaacgga aaggacaugu auacugguga ugagcugucc     2520

cuucaccggu ugucacacua cgacauugau cauaucauuc cccaaaguuu caugaaagac     2580

gauucucugg acaauuuagu acucgucggc agcaccgaaa acagagggaa guccgaugac     2640

gugccgucaa aagagguugu gaaggauaug aaggcguauu gggaaaaacu guacgccgcu     2700

ggacuuauca gccagaggaa guuucagcga cugacaaaag gcgagcaggg uggguugacc     2760

cucgaagaca aggcacacuu cauacaacgc cagcuggucg agacucggca gauuacgaaa     2820

aauguggccg gcauccuaga ucaaagauau aacgcuaauu cuaaggagaa gaaaguacag     2880

aucauuacac ugaaggccuc ccucaccagu caguuuagga gcaucuucgg acuguacaaa     2940

gugcgugaag uuaacgacua ccaucacggc caggaugcau aucuuaauug cgucguggcg     3000

acuacauugc ugaaggugua cccuaacuua gcccccgagu uugucuaugg ggaauaccca     3060

aaauuccaaa ccuuuaagga gaauaaggcu acagccaaag caauuauaua uacuaaccuc     3120

cugcgcuucu uuaccgagga cgaaccucgg uucacgaagg acggagagau ccuuuggucu     3180

aauuccuacc ugaaaaccau uaagaaagaa cuaaacuauc accagaugaa uaucguuaag     3240

aagguggagg ugcagaaagg uggcuuuuca aaggaaagua uuaaacccaa ggggccaagc     3300

aacaaacuca uccccguaaa gaauggacug gauccucaga aauacggcgg uuucgacucg     3360

ccaauagucg cuuacacagu guuguucacu caugagaagg ggaagaaacc gcugaucaag     3420

caagagauuu uaggcaucac cauuauggag aaaacaagau uugaacagaa ccccauccuc     3480

uuccuggaag agaagggauu ucuuaggccu cgaguucuga ugaaauugcc aaaguauacu     3540

cucuacgagu uccccgaagg ccggcgcaga cugcuagccu ccgcaaagga ggcccagaaa     3600

gggaaccaga uggugcugcc ugaacaccuu cucacccugu uguaucaugc uaagcaaugu     3660

cuguuaccaa aucagucuga gagccucacg uacgucgaac agcaccagcc cgaguuucaa     3720

gaaauacugg agaggguggu ugauuucgcg gaggugcaua cacuugccaa aucaaaggua     3780

cagcagauug ucaaacuguu ugaagcaaac caaaccgcug acgugaagga gaucgccgcc     3840

aguuucaucc aguugaugca guuuaaugcu augggagcac cguccacuuu caaguuuuuc     3900

cagaaagaua uugaacgggc ccgauauaca ucuauaaagg agaucuuuga cgcgaccauu     3960

aucuaccaaa gcacuaccgg ccucuaugag acaagacgca aaguggucga uuga           4014


<210>  2970
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2970
augaagaaag acuacgugau cggccuggau auugggacca acagcgucgg augggccguu       60

augacagagg acuaucagcu cgugaagaaa aagaugccca uauacgguaa uacugaaaag      120

aagaagauca aaaagaacuu cuggggcgua agacuuuuug aggaagggca cacggcugag      180

gauaggcggc ugaaacgcac cgcacgacgu agaauuucca ggcggcgcaa uagauugagg      240

uauuuacaag ccuucuuuga agaggcgaug acagaccugg augagaacuu cuuugcucgg      300

cuacaggaau cuuuccucgu gccugaggac aagaaauggc aucgccaccc aaucuuugcc      360

aagcuggaag augaggucgc auaccaugag acuuauccga ccauuuacca ccuucgaaag      420

aaacuggccg acucaaguga acaggcugau uugagacuca ucuaucuggc acuggcccau      480

auagugaagu acaggggaca cuuccucauc gagggcaaau uaagcacaga aaauaccucc      540

guuaaggacc aguuucaaca guucauggug auuuacaacc agacuuuugu caauggugag      600

ucucggcugg ugucggcucc ccuuccugaa ucaguauuga ucgaggagga acugacagag      660

aaagccagcc guaccaagaa aagugaaaag guucuacagc aauucccaca ggagaaggcg      720

aacgggcucu uuggacaguu ccugaaacuu auggugggca auaaggcaga uuucaaaaag      780

gucuuuggcc uggaagagga ggccaaaauu acguaugcuu ccgaaucuua cgaggaagac      840

uuggagggga uccucgccaa ggugggagau gaauauagcg acguguuccu ggcagcuaag      900

aacgucuacg acgccguuga guuauccacu auacuggcgg auucagacaa aaagagucac      960

gccaaacucu cuagcuccau gauugugcgc uuuaccgagc aucaggaaga ucuuaagaaa     1020

uucaagagau uuaucaggga gaauugcccc gacgaauaug auaaccuguu caagaaugag     1080

caaaaagacg guuacgcagg cuauauugcu cacgccggga agguaucaca guugaaauuu     1140

uaccaguacg ugaagaaaau cauccaagau auugcuggag ccgaguauuu ccuagaaaag     1200

auagcacagg agaacuuucu gcggaagcag cgaacauucg acaauggcgu caucccucau     1260

cagauucacc ucgccgaacu gcaagcuauc auacaccgcc aggcggcaua cuauccauuu     1320

cuuaaagaga accaggaaaa gauugagcag cuggugacuu ucagaauccc cuacuauguu     1380

gguccuuuga gcaaagggga ugccaguacc uuugcuuggc ucaagaggca aucugaggaa     1440

ccaauccggc cguggaaccu gcaggagaca guggacuuag aucaguccgc caccgcauuc     1500

auugaacgca ugacgaauuu cgacacuuac cugcccagcg agaaaguccu accuaagcau     1560

ucgcuccugu augaaaaauu uaugguguuc aacgagcuua caaagaucuc uuacaccgac     1620

gauagaggaa uuaaggccaa uuuuuccggc aaagagaagg agaaaauauu cgacuacuug     1680

uuuaagacua ggcguaaagu aaagaagaaa gauaucauuc aguucuaucg gaacgaauac     1740

aauacagaaa ucguuacccu gucaggccuc gaggaagacc aauuuaacgc uaguuucagc     1800

acguaucagg aucugcuuaa gugugggcug acccgagccg aguuggacca cccagauaau     1860

gcagagaaac ucgaagacau cauuaagaua cugacaaucu uugaggauag acagaggauu     1920

cgcacucagu uauccaccuu caaaggacaa uuuucugcgg aagugcugaa gaagcuagag     1980

cggaaacauu acacagguug gggcagacuc agcaagaaac ugaucaacgg gauuuaugac     2040

aaggagucag gaaaaacuau ccuugauuac uugauaaagg acgauggcgu caguaagcac     2100

uauaauagga acuucaugca gcugaucaau gacucccagc ucagcuuuaa aaacgcuauu     2160

caaaaggccc agucuugcga acaugaggaa acccugucag agacagugaa ugaauuagca     2220

gguagucccg ccaucaaaaa ggggauuuac cagagccuga aaaucgucga cgagcuugug     2280

gcuauaaugg gcuacgcccc uaagcgcauu guuguggaga uggcacgaga aaaccagacc     2340

acuuccacgg gaaaacggcg uucuauccaa agauugaaga uuguagagaa ggcuauggcc     2400

gaaaucggcu cgaaucuccu gaaagagcag ccaacaacca acgaacagcu aagggauacu     2460

cgccuguucc ucuauuacau gcagaacggg aaggacaugu auaccggaga ugagcugagc     2520

cuucaccggu ugucccacua cgacauagau cauaucauuc cccaaucauu caugaaagac     2580

gauagucugg acaauuuagu ccucgugggu ucuacagaga acagaggcaa gagcgaugac     2640

guuccgucca aaaagguggu caaggauaug aaagcguauu gggaaaagcu guacgccgcu     2700

gggcuuaucu cacagaggaa auuucagcga cugaccaagg gagagcaggg cgguuugacu     2760

cucgaagaca aagcacacuu cauucaacgc cagcuggugg agacacggca gaucacgaag     2820

aauguagccg ggauacuaga ucaaagauau aacgcuaaga gcaaagaaaa gaaagugcag     2880

auuaucaccc ugaaggccuc ucucacuucc caguuuagga guaucuucgg ccuguacaaa     2940

guucgugagg ucaaugacua ccaucacgga caggacgcau aucuuaacug ugugguggcg     3000

acaaccuugc ugaaggucua cccuaauuua gcccccgagu uuguuuaugg cgaauaccca     3060

aaguuccagg cuuuuaaaga gaacaaggcc acagcaaaaa cuauuaucua uaccaaucuc     3120

augcgcuucu uuacggaaga ugagccucgg uucaccaagg acagcgagau ucuguggucu     3180

aacuccuacc uuaaaacaau aaagaaggaa cugaauuauc accaaaugaa caucgugaaa     3240

aagguggagg uacagaaagg gggauuuuca aaggaaagua uuaaacccaa ggguccaagc     3300

aauaaacuaa uccccgucaa gaacggccuc gauccucaga aguacggggg auucgacucg     3360

ccagugguug cuuacacugu gcuguucacc caugagaaag gcaagaaacc guugauuaag     3420

caagaaaucc uggguauaac aaucauggag aaaacuagau uugagcagaa ucccauuuua     3480

uuccucgaag agaagggguu ucugaggccu cgaguccuua ugaagcugcc aaaauauacc     3540

uuguacgagu uccccgaagg ccggcgcaga cuccuggccu ccgcaaagga agcccagaaa     3600

ggaaaccaga uggugcuacc ugagcaucug cuuacgcucc uguaucacgc uaagcaaugc     3660

uugcugccaa accagucuga gagcuuagcg uacguugaac agcaccagcc cgaguuucaa     3720

gaaauccucg agaggguggu agauuucgcc gaaguccaua cacuggcaaa aucaaaggug     3780

cagcagauug ugaaacuuuu ugaggcuaau caaaccgccg acgucaaaga gaucgccgcu     3840

aguuucauac agcugaugca guuuaacgca augggcgccc cguccacuuu caaguuuuuc     3900

cagaaagaua uugaacgggc gcgauauaca ucuaucaagg agaucuuuga cgcuaccauu     3960

auauaccaaa gcacuaccgg guuguaugaa acaagacgca aaguugugga uuga           4014


<210>  2971
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2971
augaagaaag acuacgugau cggccuggau auugggacca acagcgucgg augggccguu       60

augacagagg acuaucagcu cgugaagaaa aagaugccca uauacgguaa uacugagaaa      120

aagaagauca aaaagaacuu cuggggcgua agacuuuuug aggaagggca cacggcugag      180

gauaggcggc ugaaacgcac cgcacgacgu agaauuucca ggcggcgcaa uagauugagg      240

uauuuacaag ccuucuuuga agaggcgaug acagcucugg acgagaacuu cuuugcccgg      300

cuacaggaau cuuuccucgu gccugaggau aagaaauggc aucgccaccc aaucuuugca      360

aagcuggaag acgaggucgc cuaccaugag acuuauccga ccauuuacca ccuucgaaag      420

aaacuggcug auucaaguga acaggcagac uugagacuca ucuaucuggc ccuggcucau      480

auagugaagu acaggggaca cuuccucauc gagggcaaau uaagcacaga aaauauuucc      540

guuaaggagc aguuucaaca guucaugauc auuuacaacc agaccuuugu gaauggugaa      600

ucucggcugg ucucggcgcc ccuuccugag ucaguguuga ucgaggaaga acugacugaa      660

aaagccagcc guacaaagaa aagugagaag guacuacagc aauucccaca ggaaaaggca      720

aacgggcucu uuggacaguu ccugaaacuu augguuggca auaaggccga uuucaaaaag      780

guguuuggcc uggaggagga agcuaaaaua accuaugccu ccgagucuua cgaagaggac      840

uuggaaggga uucucgcaaa ggucggagau gaguauagcg acguguuccu ggcugccaag      900

aacguguacg augcggucga guuauccacg auccuggccg acucagacaa aaagagucac      960

gcaaaacucu cuagcuccau gauuguucgc uuuacugaac aucaggagga ucuuaagaau     1020

uucaaaagau uuaucaggga aaacugcccc gacgaguaug auaaucuguu caagaacgag     1080

caaaaggacg guuacgcugg cuauaucacc cacgccggga aagugucaca guugaaguuu     1140

uaccaguacg ugaaaaagau uauacaagau aucgcuggag ccgaauauuu ccuagagaaa     1200

auugcacagg aaaauuuucu gcggaagcag cgaacauucg acaacggcgu aaucccucau     1260

cagauacacc ucgccgagcu gcaagcuauu auccaccgcc aggcggcaua cuauccauuu     1320

cuuaaggaaa accaggagaa aaucgagcag cuggucacuu ucaggauucc cuacuaugug     1380

gguccuuuga gcaaggggga ugccaauacc uuugcuuggc ucaaaagaca aagugaggaa     1440

ccaauccggc cguggaaccu gcaggaaaca guugacuuag aucagucugc caccgcauuc     1500

auugagcgca ugacgaauuu cgacacuuac cugcccuccg aaaaggugcu accuaaacau     1560

agccuccugu augagaaguu uauggucuuc aacgagcuua caaaaauauc guacaccgau     1620

gacagaggaa ucaaggccaa uuuuucuggc aaggaaaaag agaagauuuu cgacuacuug     1680

uuuaaaacua ggcguaaggu gaaaaagaag gauaucaucc aguucuaucg gaacgaauac     1740

aauacagaga uuguaacccu guccggccuc gaagaggacc aauuuaacgc uucauucagu     1800

acguaucagg aucugcuuaa augugggcug acccgagccg aguuggacca cccagauaau     1860

gcagaaaagc ucgaggacau aaucaaaauu cugacaaucu uugaagauag acagaggauu     1920

cgcacucagu uaagcaccuu caagggacaa uuuuccgcgg agguucugaa aaagcuagag     1980

cggaagcauu acacagguug gggcagacuc ucuaaaaagc ugaucaacgg gauauaugac     2040

aaagaaagcg gaaagacuau ccuugauuac uugauuaaag acgauggcgu gucaaagcac     2100

uauaauagga acuucaugca gcugaucaau gacagucagc ucuccuuuaa gaacgcuauu     2160

caaaaagccc agagcucuga gcaugaagag acccugucag aaacagucaa ugaguuagca     2220

gguagucccg ccaucaagaa agggauauac cagagccuga agauugugga ugagcuuguc     2280

gcuaucaugg gcuacgcccc uaaacgcauu gugguugaaa uggcacgaga gaaccagacc     2340

acuuccacgg gaaagcggcg uucuauccaa agauugaaaa uaguggaaaa ggcuauggcc     2400

gagaucggcu cgaaccuccu gaaggaacag ccaacaacca augagcagcu aagggacacu     2460

cgccuguucc ucuauuacau gcagaacggg aaagacaugu auaccggaga ugagcugagc     2520

cuucaccggu ugucccacua cgacauugau cauaucauuc cccaaucauu caugaaggac     2580

gauagucugg acaauuuagu acucgucggu ucuacagaaa acagaggcaa aagcgaugac     2640

gugccgucca aggagguugu gaaagauaug aaggcguauu gggaaaagcu guacgccgcu     2700

gggcuuaucu cacagaggaa auuucagcga cugaccaagg gagagcaggg cgguuugacu     2760

cucgaagaca aagcacacuu cauacaacgc cagcuggucg agacacggca gauuacgaag     2820

aauguggccg ggauccuaga ucaaagauau aacgcuaaaa gcaaggagaa gaaaguacag     2880

aucauuaccc ugaaggccuc ucucacuucc caguuuagga guaucuucgg ccuguacaaa     2940

gugcgugaag uuaaugacua ccaucacgga caggaugcau aucuuaacug cgucguggcg     3000

acaaccuugc ugaaggugua cccuaauuua gcccccgagu uugucuaugg cgaauaccca     3060

aaauuccaaa cauuuaagga gaacaaggcu acugccaaag caauuauaua uaccaaucuc     3120

cugcgcuucu uuacggagga cgaaccucgg uucaccaagg acggggagau ccuuuggagc     3180

aacucuuacc ugaaaacaau uaagaaagaa cuaaauuauc accagaugaa caucguuaag     3240

aagguggagg ugcagaaagg agguuuuucc aaggaaucaa uuaaacccaa gggcccaagu     3300

aauaaacuca uccccguaaa gaacgggcug gauccucaga aauacggagg cuucgacagc     3360

ccaauagucg cuuacacugu guuguucacc caugagaagg guaagaaacc gcugaucaag     3420

caagagauuu uagggaucac aauuauggag aaaacuagau uugaacagaa ccccauccuc     3480

uuccuggaag agaagggcuu ucuuaggccu cgaguucuga ugaaauugcc aaaguauacc     3540

cucuaccagu uccccgaagg acggcgcaga cugcuagccu cggcaaagga ggcccagaaa     3600

ggcaaucaaa uggugcugcc ugagcaccuu cucacgcugu uguaucaugc uaagcagugu     3660

cuguuaccaa accaguccga aucucucgcg uacgucgagc agcaccaacc cgaauuucag     3720

gagauacugg aaaggguggu ugauuucgcc gaggugcaua cacuugcaaa aagcaaggua     3780

cagcaaauug ucaaacuguu ugaggcuaau cagaccgccg acgugaagga aaucgccgcu     3840

ucauucaucc aguugaugca guuuaacgca augggggccc cgaguacuuu caaguuuuuc     3900

caaaaagaua uugagcgggc gcgauauaca uccauaaagg aaaucuuuga cgcuaccauu     3960

aucuaccagu cuacuaccgg acucuaugag acaagacgca aaguggucga uuga           4014


<210>  2972
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2972
augaagaaag acuacgugau cggccuggau auugggacca acagcgucgg augggccguu       60

augacagagg acuaucagcu cgugaagaaa aagaugccca uauacgguaa uacugagaaa      120

aagaagauca aaaagaacuu cuggggcgua agacuuuuug aggaagggca cacggcugag      180

gauaggcggc ugaaacgcac cgcacgacgu agaauuucca ggcggcgcaa uagauugagg      240

uauuuacaag ccuucuuuga agaggcgaug acagaccugg augagaacuu cuuugcucgg      300

cuacaggaau cuuuccucgu gccugaggac aagaaauggc aucgccaccc aaucuuugcc      360

aagcuggaag augaggucgc auaccaugag acuuauccga ccauuuacca ccuucgaaag      420

aaacuggccg acucaaguga acaggcugau uugagacuca ucuaucuggc acuggcccau      480

auagugaagu acaggggaca cuuccucauc gagggcaaau uaagcacaga aaauauuucc      540

guuaaggagc aguuucaaca guucaugauc auuuacaacc agaccuuugu gaauggugaa      600

ucucggcugg ucucggcucc ccuuccugag ucaguguuga ucgaggaaga acugacugaa      660

aaagccagcc guacaaagaa aagugagaag guacuacagc aauucccaca ggaaaaggcg      720

aacgggcucu uuggacaguu ccugaaacuu augguuggca auaaggcaga cuucaaaaag      780

guguuuggcc uggaggagga agccaaaaua accuaugcuu ccgagucuua cgaagaggau      840

uuggaaggga uucucgccaa ggucggagac gaguauagcg auguguuccu ggcagcuaag      900

aacguguacg acgccgucga guuauccacg auccuggcgg acucagauaa aaagagucac      960

gccaaacucu cuagcuccau gauuguucgc uuuacugaac aucaggagga ccuuaagaaa     1020

uucaagagau uuaucaggga aaauugcccc gaugaguaug acaaccuguu caagaaugag     1080

caaaaagaug guuacgcagg cuauaucgcu cacgccggga aggugucaca guugaaauuu     1140

uaccaguacg uaaagaaaau uauacaagac aucgcuggag ccgaauauuu ccuagagaag     1200

auugcacagg aaaacuuucu gcggaagcag cgaaccuucg auaauggcgu gaucccucau     1260

cagauacacc ucgccgagcu gcaagcuauu auccaccgcc aggcggcaua cuauccauuu     1320

cuuaaagaaa accaggagaa gaucgagcag cuggucacau ucaggauucc cuacuaugug     1380

gguccuuuga gcaaagggga cgccaguacu uuugcuuggc ucaagagaca aucugaggaa     1440

ccaauccggc cguggaaccu gcaggaaacc guugauuuag accaguccgc cacagcauuc     1500

auugagcgca ugaccaauuu cgauacguac cugcccagcg aaaaagugcu accuaagcau     1560

ucgcuccugu augagaaauu uauggucuuc aacgagcuua cuaagauauc uuacacagac     1620

gacagaggaa ucaaggccaa uuuuuccggc aaagaaaagg agaaaauuuu cgauuacuug     1680

uuuaagacca ggcguaaagu gaagaagaaa gacaucaucc aguucuaucg gaacgaauac     1740

aauacugaga uuguaacacu gucaggccuc gaagaggauc aauuuaacgc uaguuucagc     1800

accuaucagg accugcuuaa gugugggcug acgcgagccg aguuggauca cccagacaau     1860

gcagaaaaac ucgaggauau aaucaagauu cugaccaucu uugaagacag acagaggauu     1920

cgcacacagu uauccacuuu caaaggacaa uuuucugcgg agguucugaa gaagcuagag     1980

cggaaacauu acaccgguug gggcagacuc agcaagaaac ugaucaacgg gauauaugau     2040

aaggaaucag gaaaaacaau ccuuggcuac uugauuaagg acgauggugu gaguaagcac     2100

uauaauagga acuucaugca gcugaucaau gacucccagc ucagcuuuaa aaacgcuauu     2160

caaaaggccc agucuucaga gcaugaagag acucugagug aaaccgucaa ugaguuagca     2220

gggagccccg ccaucaaaaa gggcauauac cagucccuga aaauugugga ugagcuuguc     2280

gcuaucaugg gauacgcccc uaagcgcauu gugguugaaa uggcacgaga gaaccagaca     2340

accucuacug gcaaacggcg uucgauccaa agauugaaga uaguggaaaa ggcuauggcc     2400

gagaucggga gcaaucuccu gaaagaacag ccaacgacaa acgagcagcu aagggacacc     2460

cgccuguucc ucuauuacau gcagaacgga aaggacaugu auacugguga ugagcugucc     2520

cuucaccggu ugucacacua cgacauugau cauaucauuc cccaaaguuu caugaaagac     2580

gauucucugg acaauuuagu acucgucggc agcaccgaaa acagagggaa guccgaugac     2640

gugccgucaa aagagguugu gaaggauaug aaggcguauu gggaaaaacu guacgccgcu     2700

ggacuuauca gccagaggaa guuucagcga cugacaaaag gcgagcaggg uggguugacc     2760

cucgaagaca aggcacacuu cauacaacgc cagcuggucg agacucggca gauuacgaaa     2820

aauguggccg gcauccuaga ucaaagauau aacgcuaauu cuaaggagaa gaaaguacag     2880

aucauuacac ugaaggccuc ccucaccagu caguuuagga gcaucuucgg acuguacaaa     2940

gugcgugaag uuaacgacua ccaucacggc caggaugcau aucuuaauug cgucguggcg     3000

acuacauugc ugaaggugua cccuaacuua gcccccgagu uugucuaugg ggaauaccca     3060

aaauuccaaa ccuuuaagga gaauaaggcu acagccaaag caauuauaua uacuaaccuc     3120

cugcgcuucu uuaccgagga cgaaccucgg uucacgaagg acggagagau ccuuuggucu     3180

aauuccuacc ugaaaaccau uaagaaagaa cuaaacuauc accagaugaa uaucguuaag     3240

aagguggagg ugcagaaagg uggcuuuuca aaggaaagua uuaaacccaa ggggccaagc     3300

aacaaacuca uccccguaaa gaauggacug gauccucaga aauacggcgg uuucgacucg     3360

ccaauagucg cuuacacagu guuguucacu caugagaagg ggaagaaacc gcugaucaag     3420

caagagauuu uaggcaucac cauuauggag aaaacaagau uugaacagaa ccccauccuc     3480

uuccuggaag agaagggauu ucuuaggccu cgaguucuga ugaaauugcc aaaguauacu     3540

cucuacgagu uccccgaagg ccggcgcaga cugcuagccu ccgcaaagga ggcccagaaa     3600

gggaaccaga uggugcugcc ugaacaccuu cucacccugu uguaucaugc uaagcaaugu     3660

cuguuaccaa aucagucuga gagccucgcg uacgucgaac agcaccagcc cgaguuucaa     3720

gaaauacugg agaggguggu ugauuucgcc gaggugcaua cgcuugcaaa aucaaaggua     3780

cagcagauug ucaaacuguu ugaagcuaac caaacagccg acgugaagga gaucgccgcu     3840

aguuucaucc aguugaugca guuuaaugca augggagccc cguccaccuu caaguuuuuc     3900

cagaaagaua uugaacgggc gcgauauacu ucuauaaagg agaucuuuga cgcuacaauu     3960

aucuaccaaa gcaccacugg ccucuaugag accagacgca aaguggucga uuga           4014


<210>  2973
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2973
augcaggacu acuaucuggg ccucgauauc gggaccaaca gcgugggaug ggccgucaca       60

gacgagaauu acaaccuucu gagaaagaaa gguaaggauu uguggggcgu uagguuauuc      120

aaagaagcuc aacccgcaac ugagcggcgc gggcaccgau ccucucguag aaggacgauu      180

cggaagucac ugcgcaaaca gcuacucaga gaacuguuug aggaagagau aaaugccauc      240

gacaaggauu ucuuucuuag gcuggacgag aguaaguauu gguuggaaga uaaaaagauu      300

gagggaaaau acagccucuu caacgacgaa aauuuuaccg auaaggacua uuacgagaaa      360

uucccuacaa ucuuucaucu gcggaaggcg cugauugagu ccgaugacaa ggaagauauc      420

cgccucuauu ucuuagcuau aaaccagaug augaaacgaa gaggccacuu ucugaucgac      480

gguaaacuuu cuaaugugga ggacauuacu ccauucaaag aaugcaucca gaacuugauu      540

aaugaggaac uggauaccga gaucgagaca gaauuuuacg acaaggccau aaaaauugua      600

ucggauuacu cacuaaagaa aaccgagaag aagaaccuca ucaaagaccu ggugaaggaa      660

acugauagca uugaggacag uaaaaagauc aucaauauuu ucaaccuucu gauauccggg      720

aaaucuaagg ucaauggaau cuuugaugac gaagagauuu uggagaagau caaagaagau      780

aacaaggagg acuucuaucu cacaagcgaa aaauacgagg aaaaucugga guauuucucc      840

caauuacugg auuacagguu ugagcucaua ucaagucuua agagcauuua ugacuacauc      900

auccugcagg aaguguugaa aggcaagaag uaucuaucuu acgcacaggu ugagcgguac      960

aaaaaccaua aggaagaccu gcguaaacuc aagcagcuga uuaaaaagua ugauaagaac     1020

ggcaaaaucu acuaucaaau uuuccgcgac gagaauuuua agaaagggua cguggccuau     1080

cuuggauacu auaagaaaaa caagaaaaau gucccgguga agaaguguga uuacgacacc     1140

uucuccaaaa acauaaaguc aacgcuggau aaaaucugcg acaaggagga ucccuuguac     1200

auugaacuca ucgacaaaau cgagaagggu ggcuuucugu uaaagcagau uguaagcacu     1260

aauaguguua uaccucacca ggugcaugaa cuggagcuag auaaaauccu cgaaaaccug     1320

gagaagaauu auccaucuuu caaagagauu uccgacgggg ucagcaagau cgauaaaauu     1380

aagaugaucu uuaacuucag aauacccuac uaugugggac cucuuaauga cuaccacaag     1440

gauaaaguga acucgaauac cuggaucguc aagaacgaag gcuuugagaa ugaaaacauu     1500

aggccaugga auuucucuaa aaucguugac gagcacgcuu gugaagagga guuuauuaag     1560

cggaugauca acgaaugcuc cuauauaccc ggugagaaag uguugccuaa gucaagucug     1620

cucuacagcg aauauauggu acugaacgag cuuaauaacc ugcgagugaa uggggacaag     1680

uugaacccaa aaauuaagaa agaaaucguc gagaauauuu acaagaaaga gcgcaaggug     1740

acacucaaaa agaucagaaa cuuccugaua aucaauaacu acguugauuc caagguggaa     1800

uuuucuggau uaagcgacgg cauuaaagca aauaugucaa guuauaucga uuucgccgag     1860

auucuggacg auaacuucga caauaacaug gucgaagauc uaaucgagca gauaacuauu     1920

caugugggca auguaaagcu ccugaccgag aaaaucucca acaaguaccc gaaucuugaa     1980

aagaagcaaa ucaacaagau uguuaacuug aaauuuaagg acugggcuag guucagccgg     2040

aaacugcucg uggggaucga gggaacagaa aaggagaccg gugaaauuaa uacgauaauc     2100

ggcuuucugc gcgagacuaa cgagaauuuc auggaauuaa ugucuaacag auauacauuu     2160

ucagaggaaa uuagugagau caaaaagaag uacauuccca auaaacugag caagcagauc     2220

gucgaugacc uuuauguguc cccugccguc aaaaagauga uauggcaagu guugaacauc     2280

aaugaugaac ucauuaaagu ucuggggaag cagccaaaga aaaucuucau ugagauggcg     2340

aggggaaagg aggaaaaacc cgaggugaag gacucucgua aaaacaagcu aaucgaacug     2400

uacaagaauc ucggcaaaga ggguaaggaa uggcugaacc ggauagagga uaaaaccgag     2460

gcagacuuuc gaaauagagc ccuuuauuug uacuacacuc agauggggag guguauguau     2520

acaggcgaaa uuaucgacau cgagcgccug uacgauaagg aauuauauga ccucgaucac     2580

auuauaccuc ggaaaaugaa gaaagacgau ucgauccaua agaaccuggu acuugucacc     2640

agaaauagca accaguacga gaagaaagac aauuauccag ugcccgaggu uauuaagucc     2700

agggaaaacg ugcgccaccu gugguacgcu uugaaaaaga aaggacucau cacggaggaa     2760

aaguauaacc gacugacccg gauuaauccg cuaucagaua aggagcugag ugaauuuauc     2820

gagcgucagc ucgucgagac aagacaaacu accaaagugg uaaaggaccu guuugaaaac     2880

uacuacacag auucuaaaau agugacuguu aaggccggcc uugucagcga guugaggcgc     2940

gacuucgaag ugcugaaaug ccgggagauc aaugauuuaa accacgcaca ugacgcuuuu     3000

cucaauauua ucguggggga cgucuggucc aagaaguuca ccucaaaccc uuauaauuuu     3060

guuaaaaaga acagagaaaa auacacacug aauaacauuu ucaccaagaa ucagaaagau     3120

cuuaggggaa acgagaucug gaauugugcc gaggguaaga agcgaauaau uaaaacucug     3180

aacaagccca gcgugcuauu cucuguggaa aauuuugagc agacgggcca acucuucgac     3240

cagaacauca ucgggaaaca ggauuauaag gaaaagacau ccuacguacc aauuaaggga     3300

gacgagcgcc ugcaggauau caguaaauau ggcgguuacc gggggauaag cggcgcguau     3360

uucuucauug ucgaacacac cgagaagaau aagagaguga aaacuaucga gucuuugccu     3420

auuuacauga aguccuacgu ugaagagaac ggacuguuag acuuuugcac caacgaaaug     3480

aaacucgugg aucccaagau cauagucaaa aagcugaauu auaagucaga gauuaucgug     3540

gacggcuuca aauaccuuau cagugggaag acagagaaca ggcugagcuu ggaaaccaau     3600

gaguaucucu acuggaacca uacucagaca acggauauua aaaagauccu gaauauuaac     3660

gagaaaauag aacuagaaaa guauaaugga gacaucgagu cggagcguga aaacauuauc     3720

aaguacaucc aaauuauacu ggagaaauuu aaacuuaaac cauucaucaa gcgccggaau     3780

cuccccaaca uugaaaucaa uuuugccgau uaugagaccg aaaaacuguu gcagcuguua     3840

guuaaccuca uuccucugac uacaaaguac guguccccag uagaccuuac cgucaucucu     3900

gguagcaagu acgcaggcaa agugcugaua ccgaagaaaa ucucaaauuu gaacgaggug     3960

auuuucauca guaaguccau uacagggauc uaugagucua gaaggcgaau aauuuga        4017


<210>  2974
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2974
augaagcagg aguacuuccu gggccucgac auggggaccg gaagccuugg uugggccgug       60

acagauucca cuuaucaagu caugagaaaa cacggcaagg cucugugggg gacgagguug      120

uuugaaucug caucaaccgc cgaggaacgg cgcauguucc gaacagcgcg uagaagguua      180

gaccggcgca acuggagaau ccagguucug caggagauuu uuagugaaga gauaagcaaa      240

guggaucccg gauucuuucu aaggaugaag gaguccaaau acuauccuga agacaagcgg      300

gaugcugagg gcaauugccc agaacucccg uacgcccugu ucguagacga uaacuauacu      360

gacaagaauu accauaaaga uuaucccacc aucuaccacc uucgcaagau gcugauggag      420

acaaccgaga uuccugacau ccgauuggug uaccucgucc ugcaucacau gaugaaacau      480

agaggucacu uucugcucuc uggggauauu ucgcagauca aggaguucaa aucaacuuuu      540

gaacaauuaa uacagaacau ccaggacgag gaacuggaau ggcacauuag ccuugaugac      600

gcagccaucc aguucgugga gcauguuuug aaggacagga aucugacacg gaguaccaag      660

aaaucccguc uaauuaagca acucaacgcu aaaucugcau gugagaaggc cauccugaau      720

cuucugagcg gaggcacggu gaaauugucc gauauauuua acaauaagga acucgacgag      780

ucagaacgcc caaaagucag uuucgcugau agcggcuaug acgauuacau ugggaucgug      840

gaggccgaac uggcggagca guauuacauu aucgcaucug ccaaggcugu auaugacugg      900

uccguuuuag uggagauccu gggaaacuca gucagcauua gugaagccaa gauaaaagug      960

uaccagaagc accaggcaga ucucaaaacu cuuaagaaaa ucgugagaca auauaugacc     1020

aaggaggacu acaagagggu cuuuguugau acagaagaga aacugaauaa cuacucugcu     1080

uauauuggua ugacuaagaa aaauggcaag aaaguggacu ugaaguccaa gcagugcacc     1140

caggccgauu ucuacgacuu ccucaaaaag aacguaauca aagugauaga ccauaaggaa     1200

auuacacaag agaucgagag cgaaaucgag aaagaaaauu uucugcccaa gcaggucacc     1260

aaggauaacg gggugauucc uuaucagguu cacgacuacg agcucaaaaa gauccuggau     1320

aaccuuggaa cgcggaugcc auucauuaaa gagaaugcgg aaaagauaca gcaacuguuu     1380

gaguuccgaa uccccuauua cgugggcccu uugaaccgcg ucgacgaugg uaaagacggg     1440

aaguuuacuu ggucggugag aaaaucugau gccaggauuu auccauggaa uuucacagaa     1500

guaaucgacg uugaggcauc cgcugaaaag uuuauccggc gcaugaccaa caaguguacu     1560

uaccucgugg gagaggaugu ccugccgaaa gacucauuag uguacaguaa guucaugguc     1620

cugaaugagc uaaacaaucu cagacugaac ggcgagaaaa uuagcgugga acuuaagcag     1680

aggauauaug aagaguuguu uugcaaauac cguaagguua cacggaagaa acuggaacga     1740

uaucucguga ucgagggcau ugccaagaaa gggguagaga ucaccggaau ugauggugac     1800

uucaaggcuu cccugacggc cuaccacgau uuuaaagaaa gacuuaccga cguccagcug     1860

ucucagaggg caaaggaggc caucguguug aauguugugc uauucggcga cgauaagaaa     1920

cuguuaaagc aacgccugag caaaauguau cccaaccuaa caacugggca gcucaaggga     1980

auauguucac ugaguuacca gggcuggggu cggcuuucca aaaccuuuuu ggaagagauc     2040

acagucccug cuccagggac uggcgaagug uggaauauua ugaccgcgcu guggcagaca     2100

aacgacaauc ucaugcaacu guuaagcaga aacuauggau ucaccaauga gguagaggag     2160

uucaacacuc ugaagaagga aacggaucuu ucuuacaaaa caguggacga auuguacguu     2220

ucacccgcag ucaagaggca gaucuggcag acccucaaag uggugaagga gauucagaaa     2280

gucaugggca acgccccuaa gcgcguuuuu guggagaugg cucgagaaaa acaagagggg     2340

aagcggagug auagccguaa gaaacagcug guggaacuau auagagccug caagaaugag     2400

gaaagggacu ggaucacuga gcugaacgca caguccgauc aacagcuccg cucugacaaa     2460

cuguuccuuu acuauauaca gaagggacgg uguauguacu cgggugagac cauucaguug     2520

gaugaacugu gggacaauac aaaauaugau aucgaccaua uuuacccaca aagcaagacc     2580

auggaugacu ccuuaaacaa uagaguacuc gucaagaaaa acuauaaugc caucaaguca     2640

gacacuuacc cccugagucu ugauauacag aaaaagauga ugucuuuuug gaaaaugcug     2700

cagcagcaag gcuucaucac aaaggagaag uacgugaggu ugguucgaag cgacgaacuc     2760

uccgcugaug agcuggccgg guuuauugaa cgccagaucg uggagacgcg gcagucaacc     2820

aaagcagucg cgacuauucu aaaggaggcu cugccggaca cagaaaucgu guauguuaaa     2880

gccggaaacg ugagcaauuu cagacagacc uacgagcucc ugaagguaag ggaaaugaac     2940

gaucuucacc augcaaaaga cgccuauuug aauauagucg ugggcaacgc uuacuuugug     3000

aaguucacaa agaaugccgc augguuuauu cguaacaauc cuggucgcuc uuauaaccug     3060

aaacggaugu ucgaguuuga uaucgaaaga uccggggaga ucgcuuggaa ggccggcaac     3120

aaaggaagua uugucacugu uaagaaagug augcaaaaga auaacaucuu aguaaccagg     3180

aaggcguacg aggugaaagg cggguuguuc gaccagcaga uuaugaagaa aggaaagggu     3240

caagucccca uaaaaggcaa ugaugaacga cuggccgaca ucgagaagua ugggggauac     3300

aacaaagcug caggcacgua cuuuaugcuu guuaagagcc uggauaagaa agguaaggaa     3360

auucggacca ucgaguucgu gccacuauau cucaaaaauc agauugagau caaccacgaa     3420

ucugccauac aguaccuggc ucaggagcgc gggcucaauu ccccugaaau ccuguuauca     3480

aagauuaaaa ucgacacacu cuucaaggug gacggcuuua agauguggcu gaguggaaga     3540

acuggcaacc aacuuauuuu caaaggggcc aaucagcuga ucuugagcca ucaggaggca     3600

gcgauacuca agggagucgu gaaauauguu aacaggaaga augaaaacaa agaugccaag     3660

cugucggagc gggacggcau gaccgaggaa aagcuacugc agcuuuacga uacauuucuc     3720

gacaaacugu ccaauacugu guauucuauu cgauugagcg cucaaaucaa gacccugacg     3780

gagaaaagag ccaaguucau cgguuuauca aacgaagauc agugcauugu ccucaaugag     3840

auacugcaca uguuucagug ucagaguggg uccgcaaacc uuaaacugau cggaggcccc     3900

gguucugcug gcauuuuggu aaugaacaau aacaucacag ccugcaagca aauuagcgug     3960

aucaaucagu ccccaaccgg gauauacgaa aaggagaucg accucauuaa acuguga        4017


<210>  2975
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2975
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

guuacugaug guuauaaagu gccugcuaag aaaaugaagg uacucggcaa uacggacaaa      120

ucucacauaa agaagaaccu ucugggggca uuguuauucg auucaggaaa uaccgccaaa      180

gacagaaggc ugaagcggac agcgcgccga cguuacacua gaaggcggaa ccgcauccua      240

uaucuccagg agauuuuugc ugaagagaug gccaaagcag augaaaguuu cuuucaaaga      300

cugaaugaga gcuuccuuac caacgacgau aaggaauuug acucccaucc aaucuucggc      360

aauaaagccg aggaggaagc ucaccaucac aaguuuccga caauuuucca ucugaggaag      420

cacuuggcag auucuaccga gaaaucggac cuccggcuga ucuaccuggc ccucgcucac      480

augauaaagu uucgcgguca uuucuuaauc gaagggcagc ugaaagccga gaacacuaau      540

gugcagacac uuuuugauga cuucgucgag guguaugaua agaccguuga agagucacac      600

uugagcgaaa uuaguguguc cucuauccug acggagaaaa uuagcaaguc ccgaagacua      660

gaaaaccuca ucaaauacua ucccacugag aagaagaaua cccuguuugg aaaccuuaua      720

gcgcugucau ugggccucca gccuaauuuc aaaacaaacu ucaagcugag ugaggacgca      780

aaauuacaau uuagcaagga cacuuacgaa gaggaucugg gcgaacuccu ugggaaaauu      840

ggagacaauu acgccgaucu guucaucucu gcuaagaacu uguaugacgc cauucuacug      900

uccgguaucc ucaccaucga ugacaacaca accaaggcac cacugucagc uagcaugauu      960

aaaagguacg aggaacauca ggaggaucuu gcccagcuga agaaauuuau acggcagaau     1020

uugcccgacc aauauaguga ggucuucucu gauaagacga aagacggcua cgcgggguau     1080

aucgauggaa agacuaacca ggaagccuuu uacaaguaua uuaaaaauau gcucuccaag     1140

acagagggcg cagacuacuu ccuggacaaa aucgaucgug aagacuuuuu acgcaagcag     1200

agaaccuucg auaacgguag cgugccucac cagauacauc ugcaagagau gcacgcuauu     1260

cuaaggcggc agggggaaua cuauccauuu cucaaagaga aucaggacaa gaucgagaag     1320

auccugacuu uccgaauacc cuacuaugua ggaccucuug cccgcaaagg cucgagauuu     1380

gcuugggccg aauacaaggc agauaaaaag guuacaccau ggaacuucga cgauaucuug     1440

gacaaagaga agucugccga agaguuuauu accaggauga cgcugaauga ucucuaucug     1500

ccggaggaaa aagugcuucc caagcacucc cuggucuacg agaccuucaa cguguauaau     1560

gaauugacaa aggugaaaua cgucaacgag caaggcaagg cuauauucuu ugacgcgaau     1620

augaaacagg aaaucuucga ucauguuuuu aaggagaacc ggaaagugac uaaggacaag     1680

cuccugaauu acuuaaacaa agaguucgaa gaguuucgca uuguaaaucu gaccgggcua     1740

gauaaggaaa acaaagcauu caacucaagu cucggaacau aucacgaccu gagaaagauc     1800

cuugacaaga gcuuuuugga ugacaaggug aaugagaaga ucauugaaga uauaauccag     1860

acucugaccc ucuucgagga cagggagaug auucgucagc ggcugcaaaa auacuccgau     1920

aucuuuacaa cccagcaguu aaagaaacug gaacgaagac auuauacugg uuggggcagg     1980

cuuucugcca aguugauuaa cgggauccgc gacaaacaga gcaauaagac gauacucgga     2040

uaccugaucg augacggcua uucaaaccgg aauuucaugc aacuaauuaa cgaugacagu     2100

cugccuuuua aggaggaaau cgcuagagcc caggucauug gugagacaga ugaccucaau     2160

cagcuggugu ccgauaucgc agggagccca gccauaaaga agggcauucu ucagucuuug     2220

aaaaucguug acgaacuggu gaaggucaug ggacacaacc ccgcuaauau ugugaucgag     2280

auggccaggg agaaccaaac cacugcaaaa ggccgccgau caagucagca gcgguacaag     2340

cguuuagaag aggcgauuaa aaaucucgac cacgaucuga accauaagau ccuuaaggaa     2400

cacccuaccg acaaucaggc ucugcaaaac gauagauugu uccucuauua ccugcagaau     2460

gggagggaca uguacacaga ggauccacua gacauuaacc gccugagcga uuaugacauc     2520

gaucauauua ucccccaguc cuuuauaaaa gacgauucua uugacaacaa gguacucguu     2580

ucgagcgcca aaaaucgggg aaaguccgac aacgugccgu cagaggaugu cgugaauaga     2640

augaggccuu ucuggaacaa acugcuuagu ugcgguuuga ucucucaacg aaaguacagc     2700

aaucugacca agaaagaauu aaagcccgac gauaaagcag gcuucaucaa gcgccagcuc     2760

gucgagacuc ggcagauuac aaaacacgug gcccagaucc uggacgcuag auuuaacacg     2820

aagagggaug aaaauaagaa aguuauucgu gacgugaaga uaaucacccu uaaauccaac     2880

cugguaucac aauuccgcaa ggauuuuaaa uucuauaagg uccgggagau uaaugacuac     2940

caccaugccc acgaugcaua ucuaaacgcu gugaucggga aggcccuccu ggacguuuac     3000

ccacaguugg aaccugaguu uguguaugga gaguaccccc auuuccacgg cuacaaagaa     3060

aauaaggcga cugccaaaaa guuuuucuau agcaacauua ugaacuuuuu caaaaaggau     3120

gacaucagaa cagaugagaa uggugaaaua gucuggaaga aagacgagca uaucucuaau     3180

auuaagaggg ugcuguccua cccacaggua aacaucguga aaaagguuga aauucagacc     3240

gucgggcaaa acggcggauu auuugacgau aaucccaaaa guccucucga ggugacacca     3300

agcaagcugg ugccgcuuaa aaaggagcug aaccccaaga aauauggcgg guaccagaag     3360

ccuacuaccg cuuauccagu cuugcucauc acggacacca aacagcugau acccauuucu     3420

guuaugaaua agaaacaguu cgaacaaaac ccugugaagu uccuacgaga ucggggauac     3480

caacaggugg guaagaauga cuuuaucaaa cugccaaagu auacacuugu agauaucggc     3540

gacgggauua aacgccucug ggcauccuca aaggagauac acaaaggaaa ccagcugguc     3600

gugaguaaaa agagccagau cuugcuguac cacgcccauc acuuagauuc ggaccucucc     3660

aaugauuacc ugcagaacca uaaucagcaa uucgacguuc uuuuuaacga aauuaucucu     3720

uucagcaaaa aguguaaacu gggcaaggag cacauucaga aaaucgaaaa uguguauuca     3780

aacaagaaga auagugcuuc cauagaggag uuggccgaau cuuuuaucaa acuccugggu     3840

uucacucagc uaggggcaac cagccccuuu aacuuccugg gcgucaaacu uaaucagaaa     3900

caauacaagg gcaaaaagga uuauauucuc ccgugcacag agggaacucu gaucagacag     3960

uccauuaccg gguuguacga aacgagggug gacuuaucaa aaaucggaga ggauuga        4017


<210>  2976
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2976
augagagacu acuauaucgg ccuggaucuc gggaccggaa gccuugguug ggccgugaca       60

gacagggagu acgaaauuau gcgggcucac ggcaaggcac uguggggagu ccgcuuguuc      120

gauuccgcca acacugcgga ggaacgacgu ggguuuagaa cggcuaggcg gcgcuuagac      180

agaaggaauu ggcggauaga gcugcuacag gaacucuucg gcgaggauau cgguaaaguu      240

gacucugggu uuuuccugcg caugaaggag ucaaaauaua ugcccgaaga uaagcgagac      300

gugaacggaa auugcccuaa acuuccauac gcccuguuug uagaggaugg cuauaccgac      360

aaggauuacc auagacaauu cccgacaauu uaucacuuga ggaaguggcu cauggaaacu      420

gaggagaccc ccgacauccg gcugguguac cuggcacucc aucacaugau gaaacaucgu      480

ggccacuuuu uauucagugg gaacauugaa aagaucaaag aguuucagga aacauuccgc      540

caguacauag gaaagaucag agaggaagaa cuggauuuuc accuuuguau ugagggugaa      600

gaguugaggg aaaccgagaa uauccugaaa gacaagaacc uaacucggag cgccaagaaa      660

acacgacuca uuaagcugcu uggcgcucau accgcaugcg aaaaagccgc ucugaauuug      720

gucgccgggg gaacggugaa gcucuccgac aucuucggca acucugagcu ggaugcgugu      780

gagaaaccua aguuaucguu ugcagacgcc gguuaugaug acuacgcugg gaugauagaa      840

gaugagcugg gagaacagca cguuauuauc gagacugcca aagcagugua ugacugguca      900

guccucgcug auauucuugg cgacuaccgc ugcaucagcg aagccaaggc ggccguguau      960

gagaagcauc aaaaagaucu gagacacuug aaggagcuag uaaaagaaaa ucugggcagg     1020

gacguuuaca aggagguguu cgucaaaacc aacgaaaagc ucccaaauua uagugcauac     1080

aucgggauga caaagaaaaa cggagugaag uccgagaugg aggguaaacg gugugaucgc     1140

aaggcuuucu acgacuaucu gaaaaagacu guggucaaug ccauucccga cgaaucuaag     1200

accgaguacc uuagaaaaga aauggagaca gaaaccuuuc ugccuaggca gguuacgaag     1260

gauaacggcg ugauaccaca ccagguacau uugcaggagc ucgacgcuau ccuggagaau     1320

uuaagcgggc guauucccgc ccugaaagaa aacggaucca agauccggga uauauucacu     1380

uuucgaaucc cuuauuacgu gggcccacua aacgguauug ucaaaggggg cgagagaaca     1440

aauuggguga ggcgcaagaa ggcaggacgg aucugcccgu ggaacuucga cgaaaugguu     1500

gauaccggcg ccucagcuga ggaauuuauu agaaggauga caaguaaaug uacuuaucuc     1560

auccacgagg acgugcugcc caagaauagc augcuuuacu cuaaauucau ggucuugaac     1620

gagcugaaua acgugcgccu caauggggaa ccuauuuccg uagagcugaa gcagaaaacc     1680

uaugaagauc uuuuucaacg acaucggaag guuacgcgua gaaggcugac cgacuacaua     1740

cgccgggagg gaaucgcggg uagagaugca gacauuacag gcaucgaugg ggacuucaaa     1800

ggaucauuga cugccuacca cgauuuuaag gaaaagcuca ccggcugcga gcugagccag     1860

gcugacaaag agaacaucau uuuaaauaua acacuguucg gugaagacaa ggcccuacuc     1920

aaaaagaggc ugggggcacu uuauccagcc uugacugagc cccagaaaaa ggcuaucugu     1980

gcccugaguu acaagggcug gggacgacuc ucucaacgcc uguuagaagg cauuaccgcg     2040

ccugcaccag agacagggga gaucuggacc gugauucggg cuauguggga aacuaacgau     2100

aaucugaugc agguccuuuc cgagaaauau ugcuuugccg cagccaucga cgaagagaac     2160

gcuggagaag aguugaagga gauaacguac aaaacagugg aacagaugaa ugucagcccc     2220

gccgugagaa ggcaaaucug gcagucgcuc cagguuauua aggagaucug uaaagugaug     2280

gguggcccgc cuaagcgugu auucgucgaa auggcacgcg agaagaugga aucuaaacgg     2340

accgagucca gaaagaaaag gcugauugau cuauauaaga aaugccgaga ggaagaacgg     2400

gacuggaucg aggagcuggg gaacacugaa gagacccgcc ucagaucaga uaagcuguac     2460

cuuuauuaca cacagaaggg aaggugcaug uacaguggcg aggugauaga auuggaggaa     2520

cugugggaca aucggaaaua ugauauugac cauaucuacc cccaaagcaa gguuauggau     2580

gacuccuuag auaaccgagu gcucgucaaa aaggaguaua acgcugacaa aaccgaugag     2640

uacccaauua gagccgacau ccgcgguaag augagggcgu uuuggcguau acugcgggaa     2700

gagggguuca ucucuaaaga aaaguauaau agacuuacua ggggcacagg auucgagccu     2760

agcgaacugg ccggcuuuau ugcucgccag uugguggaga cgcgacaggg gaccaaggca     2820

guagccucag ugcucaaaca gguuuucccc gagacugaca ucgucuacgc uaaggccaga     2880

guggcaaguc aauuucggca ggaguucgau cugauuaaag ugagggagau gaacgaccua     2940

caccacgcga aggaugccua ugucaauauc guugugggaa acguguacua cacaaaauuu     3000

accuccaaug cugccuggua uguaaaggaa cauccaggcc gcagcuacaa ccugaagaaa     3060

auguucacau cugagcguga cgucgcaaga aauggugaaa cugcuuggcg ggccgggaac     3120

ucaggaacca uagcaacggu gaagaggguu augggcaaaa auaacauucu cgugacccgc     3180

cgaaguuaug aggucaaggg uggccuguuu gaucagcagc uuaugaaaaa ggggaaggga     3240

caagugccca ucaaaggcag agacgagcgg uuggccgaua ucgacaagua cgggggauau     3300

aauaaagcug cgggcacaua cuucaugcug gccgaaagcg aggauaagaa aggugcaaag     3360

auuagguccg uugaauaugu gccuuuauac cucuguaacu gcaucgagaa ggacgaagag     3420

gcugccaaaa aguaccugca gaaagagcgc gggcuuaaga auccaagagu acugauugcc     3480

aaaauaaaga ucgauacucu auuuaagguc gacggauucu auauguggcu cucuggcagg     3540

accgguaacc agcugauuuu uaaaggcgcu aaucaguuga uccugucgga accggacaug     3600

cggauuuuaa agaaagugcu caaguacgug aaccguaaaa aggagaacaa aaaugcaguc     3660

cugggggaac acgaucaacu ucccgagaca gaccugaucc gcuuguauga uguuuuccuc     3720

gacaagauag agaacacugu guaccaugua cgacugagcg cccagcaggg aacccuaacg     3780

aagaauaaag auacauuuug ugaacugucc aacgaggaca agugcaucgu gcuuucagaa     3840

auucuccaca uguuccaaug ucagaguggc ucugcgaauc ugaaauugau caaggggccu     3900

ggaagcgcug gcauucuggu cuuaaacaau aucauaucca aaugcaacca ggugucaauu     3960

auccaccaga gcccaaccgg uaucuaugag caagaaauug aucucaagaa gauauga        4017


<210>  2977
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2977
augaacaagg ccuacacccu gggccucgac aucgggacaa auagcguggg augggcuguc       60

guuacugaug acuauagacu uauggcgaaa aagaugcccg ugcacuccaa aauggagaag      120

aaaaagauua agaaaaacuu cuggggugcc aggcuguuug augaaggcca gacggcggag      180

gaacggcgca auaagcgagc uacccguaga agguugcggc gcagaaaaua caggauauua      240

gagcugcaaa agaucuucuc ugaagagauu cuaaagaagg acucacacuu uuucgcccgg      300

cucgaugaga guuuucugau cccugaagac aagcaguaug cacgcuuccc aauuuuuccg      360

acacuucugg aggaaaaagc cuacuaucag aacuacccca cuaucuauca cuugcgacag      420

aagcucgcug auagcaccga gaaagcagac auaagacugg uauaccuggc ccucgcucau      480

augaucaagu acagggggca cuucuuauuu gagggagaac uggauacaga gaauaccucc      540

guggaagaga cuuucaaaga auuuauugac aucuauaacg agcaauucga ggaaggcauu      600

aucuuuuaca aggauauacc ucuuauuuug acagacaaac ugucuaaguc gaagaaaguc      660

gagaagaucc uacaguauua cccaaaagaa aagaccacgg guugccucgc ccaguuccug      720

aaacuuauug uggggaauca gggaaacuuc aagcaagcgu uucaucugga ugaggaaguu      780

aagauccaga ucucaaaaga gacuuaugag gaagacuugg agaagcuccu gcggaaaagc      840

aaugaagaga ugauugacgu guucuuacag gucaagaaag uguacgaugc aauacugcuc      900

agugacaucc uuuccaccaa gaugaaggau acaaaagcca agcugucugc uggcaugauu      960

gaacguuauc agaaccacaa aaaggacuug gaggagcuaa aacaauuugu acgcgcccau     1020

cugcaugaaa agguuacugu guucuuuaag gauagcucca aagacggcua cgcaggguac     1080

aucgauggaa agaccacaca ggcugacuuc uauaaauuuc ucaagaaaga gcugaccggu     1140

guccccggcu cagaaccuau gcuugccaag auagaucagg agaauuuccu guugaaacaa     1200

agaacgccaa cuaacggggu gauuccccac caggugcauc ucacagaguu uaaggcgauc     1260

aucgaccagc agaagcaaua cuauccuuuc cuggaaaaga guaaggagaa aaugauucag     1320

uuacugaccu uuaggauccc auacuauguc ggaccgcuag cccaggauaa ggaaacuagc     1380

ucuuucgcau ggcucgagcg gaaaacaacc gaaaagauua agcccuggaa ugcuaaagac     1440

guuauagacu acggcgccuc cgcuacgaag uuuauccagc gaaugauuaa cuaugauacc     1500

uaccugccua cagagaaagu gcuuccaaag uacucaaugu uguaucaaaa auacacuauc     1560

uucaaugagc ugaccaaggu agccuauaag gacgaucgcg guaucaaaca ccaguucagc     1620

agugaagaga agcucagaau uuuucaggaa cuguucaaaa agcagaggcg ggugacaaag     1680

aagaaacuuc aacacuuucu gucugcaaac uacaacauag aggacgccga aaucuugggg     1740

gucgauaaag uguucaauuc cagcuaugcu acuuaccaug acuuucucga gcuggcgaag     1800

cccuauaccg agaaaauuau cgaauuacug gagcagccug aaauggagga aauguucgag     1860

gauauuguua agaucauaac aaucuuugag gaccgcgaaa uggugagaac ccagcucaaa     1920

aaguaccaga ggauucuagg agaggaaauc uucaaaaagc uggucaagaa aaaguacacu     1980

ggcuggggcc gucuuucgaa acgguugauu aacgggaucc gagaucaaaa gacgaauaaa     2040

acaauacugg acuaucucau uaacgaugac gauuuuccau acaauagaaa caggaauuuc     2100

augcagcuga ucaacgacga ccacuuaucu uuuaaggagg agauugcaaa ggaacugacc     2160

cuuuccgaua aacagucauu gcucgaggug guagaagcca uccccggaag uccggcuaua     2220

aagaaaggua ucuggcaaac ucugaagauu guugaggaac uaaucgccau uaucggcuau     2280

aaaccuaaga auauagugau ugagauggca cgcgagaacc agaccacaac cgggggaaag     2340

aaucggagca aacccagacu gaagucccuc gaagaggccc ugaaaaacuu cgacucucag     2400

cuuuugaagg aaaggccagu cgauaaucag agccugcaaa aagaccgcuu auaccucuau     2460

uaccugcaga acggcaagga uauguauacu ggugagucac uugacaucga ucgacugagu     2520

gaauacgaca ucgaucauau uaucccucgg uccuuuauug uggaccacag cuuggauaau     2580

aagguccucg ugucuucaaa agagaaccgu cugaagaaag acgauguucc cgacaguaag     2640

gugguaaaaa gaaugaaggc uuacugggag aagcuacuga gggccaaccu cauaagcgaa     2700

cgcaaauucu ccuaucugac aaaacuugag uugacggacg augacaaagc acgguucauc     2760

cagagacagc uggucgaaac caggcaaauu acuaagcaug uggcggcuau cuuacaccag     2820

uacuuuaauc agacacagga gcucgaaaaa gagaaggaua uucgaaucau aacccugaaa     2880

ucuucgcuug uuagccaauu ccgccaggug uuugggaucc acaagguccg ggagauuaac     2940

caucaccauc acgcccacga cgcauaucug aaugccgugg uagcucuagc ccuccugaag     3000

aaauacccaa gauuggcacc cgaguucgug uauggcuccu uugcuaaguu ccaucugguu     3060

aaagaaaaca aggccacagc gaaaaaggaa uuuuacucaa auaucuuaaa guucuuugag     3120

aaagaggaac aguucuguga ugagaacgga gaaaucuuuu gggacaagag gaaacacauu     3180

caacagauaa agaaagucau uaguucucau caggugaaua ucgugaagaa ggucgagguu     3240

cagacuggca gcuucuauaa agaaaccgug aacacgaagg agaaaccuga uaagcucauc     3300

aaacguaaga auaacuggga cgugaccaag uacgggggau ucgguucccc aguagucgcc     3360

uacgcugugg uuuuuacaua ugagaaaggc aagaaucaca aaaaggcaaa agccauugaa     3420

gggauaacua ucauggagca agcucuguuc gaaaaggauc cgauuucauu ucuuaucgag     3480

aaaggauaca gcaacgugaa ucaguucauu aagcugccca aguauaccuu guuugaacuc     3540

gccaacggcc agcgccggau gcuggcaucu caucaggagc uacaaaaagc aaauuccuuc     3600

auccugccug agaaacuugu cacacuccug uaccacgcca accacuauga cgaaauagcu     3660

uacaaagaua guuaugacua cgugaacgag cauuuuagca auuuccagga caucuuggau     3720

aagguuauua ucuuugccga aaaauacacu ucugcaccac agaagcugaa ccagauuauc     3780

gcuaccuaug agaagaauca agaagccgac agaaaaauaa uggcacacuc cuucgugaac     3840

uuaaugcagu uuaaugcccu cggugcuccc gcggauuuca aguuuuucga cacgacaauu     3900

accaggaaac gauacacuuc acugacagag aucuggcaga guaccaucau uuaucagagc     3960

guaacugggc uuuacgagac ccggcgcaga auggccgauc ugugggacgg cguccaauga     4020


<210>  2978
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2978
auggagagau accacaucgg ccuggacauu gggaccagcu ccauaggaug ggccgugauc       60

ggugaugacu ucaagauuaa aaagaaaaag ggcaaaaacc ucaucgggac aaggcuuuuu      120

aaugaaggag cuacugcagc cgagcggcgc ggcuuccgaa cgcagcguag aaggcugaac      180

cggcgcaagu ggagauugaa guuacuggaa gagauuuuug aucccuauau ggcggaaguc      240

gacgaguacu ucuuugcuag gcuaaaaucu ucaaaucuca guccuaagga uagcaacaaa      300

aaguaucugg guucccuucu guucccagac aaaucugauu cgaauuuuua cgacaaguau      360

ccgaccaucu accauuugcg gcgcgaucuc auggagaagg acgaaaaauu cgaucugcga      420

gagauauauc uggccaucca ccauauuguu aaguacagag ggaacuuucu cgaaaaagug      480

cccgcaaaga auuacaaaaa cucaggagcc agcaucggcu ucuuacugga ggagguaaau      540

agucuuuaua aggacauuau cggcgaugaa uccauagcua uuuugaacuc ugagaaauuu      600

gaagacgugg agaagaucau ucuggacgaa gagacaagga aucuagauaa gcaaaagagc      660

gucgggaagc uccuggugga ggacaaaaag aaaaagaaca ucguuacugc auucuccaag      720

gccaucuuug gauacaaauu caauauugaa gaucuucugu ugauagagaa cgacgaaaag      780

aauaaacuca ccuucaacga ugagaacauc gacgauauuu uuaaugaacu gucacacagu      840

uuagacgaua agcagaugga ccugcucaca aaaacccggg agaucuauuu caaguuuaaa      900

cuuaacauga uagugccuac ugguuacaca cugagcgagu cuaugauuga aaaauaugag      960

augcauaagg cucacuugaa aauguacaag gaguucauca auacccuaaa cacgaaagau     1020

cguaagaucc ugaagaaugc cuauuccgac uacauuaaca cugagaaagc gaaggcagcc     1080

aaugcucagg aaaacuuuua caaaaccguc aagaaaauca uuaaggacaa uaacucagau     1140

acagccaaaa agauaaucgu gcucauugac gagggcaauu ucaugccaaa gcagcgcacu     1200

ggggagaacg gaguaauccc ccaccaacug caucagaucg aacuugauag aauuauagag     1260

aaucaggcaa aauauuaccc uuggcuggcu gaagccaacc caguugagaa gaauaggaaa     1320

uuugcgaagu auaaauugga cgagcucgug accuuccggg uccccuacua ugugggcccu     1380

cugaucgaua agacagaaag caacaagaac gagaaagaaa ccaaguuugc cuggauggug     1440

cgaaaagcaa aggguacgau uacuccaugg aauuucgaga acuuagucga ccgcacagaa     1500

agugcuaaua gauuuaucaa aaggaugacc ucuaaggaua cuuacauuau cggggaggac     1560

guucugccgg ccuccagccu acucuaugag aaguucaaag ugcugaacga acuuaauaac     1620

auaaagguaa augagaaaaa guuggauauc gaacagaaac aagacauuua ccugaaccuc     1680

uuuacaaccg cuaagaaugu gacgaagaaa ucgcuggcca ccuaccuuaa cugcucugca     1740

gauuccaucu caggacugag ugacggcgag aaguucaaua gcuccuuguc uagcuauauu     1800

gaucucaaag ccauccuggg caacauaguc gacgacugua auaagaacga agauuuagag     1860

aaaauuaucg aguacucaac aguguucgaa gacgggaaua uuuauaagga gaaacugagu     1920

gaaaucuccu ggcuaacuga ugagcagaua gaaaagcuca gcaacaucca cuuuaaggga     1980

uggggucggc ugucuaaaaa guugcuuacc cagauuacaa acgagaaugg cgagcgcauc     2040

auugacgcuc uguggaacac uucaaauaac uucaugcaga ucauaaauga ugaaaguauu     2100

caagcgaaac ucgcagagau caacgggaag uacgccggaa aaagcgaccu ggaagauauc     2160

uuauccgagg cuuauaccuc uccccagaau aagaaggcca uuagacaggu uaugaaagug     2220

gucgaggaca ucgaaaaggc aaugcaaugc gagccuacau cgauuagcau agaauuuacc     2280

aggaaaaagc agaaaucccg ucugacuaac acgcgguaca agaagaucuc agauguguau     2340

gagaaaauug ccgacgaagu aaucagugag uacgagcuuc gaaaguugca gucugaacuc     2400

gauagcaaug cuaacaauau gagagacagg uacuaucugu acuucaugca gcuaggccgc     2460

gauauguaua caggugagaa aauuaacauc gacgaacugc aucaacggua cgacauagau     2520

cacauccucc cacaguccuu uauuaaggac gauucaauca auaacagagu ucugaccagg     2580

aaagacguga auaacaagga aaagagcgau aaaacugccg cagaccuuua ugcggucgaa     2640

augucugauu ucuggcgcaa guugcgaaaa caggggcuga uuaccgagca gaaguacaaa     2700

aacuuacuca caaaguccga caguaucgau aaguaugcua aaaagggcuu uauaaaacgg     2760

caacuggugg agaccagcca ggucgugaaa cuuguugcca auauucugca ggacaaguac     2820

ucugauacua agaucaucga agugcgugca aaauugaacu ccaaucucag agaggaauac     2880

aagcugauua agaacagggc cguaaaugac uaucaccaug cuauagacgg auaccuaaca     2940

acguucaucg gccaguaucu guacaaaacc uaucccaagc uccgcucaua cuuugucuau     3000

aacgauuuca aaaagcugga caguaauuac cuuaaacaca ugaagaaguu uaacuucuug     3060

uggcaacugc aggaugacaa aacuauugau aucuacgaca augugaacaa uaaguuuguu     3120

uuaaacgugc cugagaugaa aaaguauauu cagaaaaucu acaauuauaa guacaugcuc     3180

gucagcaagg aagugacaac caaaaacggg gccuucuaug aucagacaaa guacaaugca     3240

aaaacuguaa accugauacc aaucaagaaa aauaagccca ccaacauuua uggagguuac     3300

aagggcaaag ugucguccua caugaugcuu guuaagaucc ggaaaaagaa agaggucauu     3360

uauaaguucg ugggggugcc gagacugugg acggacgagc uagauaggcu caaagacacc     3420

acagaacgaa agauccuguu gaacgagaua gcuaaaaauu cucugagcaa gacugaacaa     3480

gauuuugagg ucauuuuaga caagguuuac uauggacagc ucaucaucga uggcaaccag     3540

aaauacaccc uggguucaag ugaguauaag uacaaugcca ugcaacuuca ucuguccaaa     3600

cacgcguugg aagugcucgc caaggagaac gugaaggacg cugaaauuac agacaaagau     3660

cugguaucug ucuaugagga aauccuaagc gugguuaaua aguacuucga gcuguacgac     3720

auaaacaaau uucgccagaa acuuaaugag gggcucgaaa uuuuccggaa gcugccugug     3780

uauaacgucu acgaguccaa uaagaucaaa caggugggca aguuugaagu uuugaacagg     3840

auucugaugg gauuacaugc aaaugccaug aucacugauc ucaaggugcu gggcauaaaa     3900

accaaacuug ggcagaugca aguaaacgga gguauuaagc ugucacccga cgcuaaguug     3960

aucuaucaga gcccaacggg caucuucagu agagccgucc gugugaagga ucucggguga     4020


<210>  2979
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2979
augagccuga acaccaauaa gaaauacaac aucggccucg acauugggac aacuuccgug       60

ggaugggccg ucacggaugc ugacggucag aaacuucuga aagcaaagaa uaaaaacuug      120

uggggcguuu cuuuauucga ggccgggaag accgcggcug aaagaagggg auaucgguca      180

acacgccgac gucugaauca cagaaaguuu aggcuacggc uccuggagga uauguucgaa      240

aaagagauac uuaguaagga ccccagcuuu uucauccgcc ugaaagaagc cuuuuugucc      300

ccuaaggaug agcaaaaaca guucaagggc aacauucucu uuaaugacaa ggauuacacu      360

gacgcagauu auuacgagca guauaaaacc aucuaccauc ugagauauga ccugauuucu      420

cagcacaggc aauucgauau ccgggaagug uaccucgcca uacaucacuu aaucaaguac      480

cgcggucauu uuauuuauga ggaccagaca uucaccacug augggaacca gcugcagcac      540

cacaucaaag cuauuaucac aaugauaaau ucgauucuua accgaaucau uaucccagaa      600

accaucgaca uuaauguauu ugagaagacg uugcuggaca gaacuaugaa caggucaagc      660

aaagugaagu uccuaauaga gcucaccgga gaagauaaac aagacaagcc gcugcuuaag      720

gagcuguuua auuugaucgu cggccucaaa gcaaagcccg ccaguauuuu cgaacaggag      780

aaccuggcua caaucgugga aacuaugaau auguccaccg agcagguuca guuagaucug      840

cucacacuug ccgacgugcu ggcggaugag gaauacgacu ugcuacugac cgcacaaaag      900

auauauucug ccauuauccu cgaugagagc auggacggcu acgaauauuu cgcugaggcc      960

aagaaagaau ccuaccggaa gcaucaggag gagcuggucc uugugaagaa aaugcugaag     1020

ucaaacgcaa ucacgaacga ugaacgugcu aaauuugagu auuucuacac ugacuacauu     1080

ggggccaaga guagcaauua ugaagagucu aaaaucauua agaagggauu guccgcggcc     1140

uacgguaaau auucaaagga agaacgccuc uuuaaacaca uagagcuguu acuggcaaag     1200

gaaaacguac uaacaaccgu ugagcaugau cuccuggaaa agaauaucac uuucgcuagc     1260

cuuuugccuc ugcagagaag uucuaggaac gccgugauuc cauaccaagu ccacgagaag     1320

gagcucgugg cuauccugga aaaucaggcc acauauuacc ccuuucuucu ggagcagaag     1380

gacaacaucc acaaauugcu caccuuccgg auuccuuauu acgugggccc acuggcagac     1440

cagaaggauu ccgaauuugc cuggaugguc cgaaaacaag cugggaagau aacgcccuuc     1500

aauuuugagg aaaugguuga caucgaugcg agcucggagg cauucauuaa acgcaugacc     1560

aacaagugca cauacuuaau ccaugaggac gugauuccua aacacucuuu uuccuaugcc     1620

aaguucgaag uacugaauga gcuaaacaag aucagacucg auggaaaacc aauagacauc     1680

ccgcugaaga aaaggauuuu ugaaggccuu uucuuggaga agacuaaagu gacccauaca     1740

ucacugaaga aguggcucgc ugaauacgag cacaugacug ucaguguggu ucaggguacc     1800

cagaaagaga cagaguucgc caccagccug caggcauuuc aucgguucgu gaagaucuuu     1860

gaucgcgaga cugucuccaa ucccgccaac gaagagaugu ucgagaaaau uaucuauugg     1920

ucuacggugu uugaagacaa gaaaauaaug agaaggaagu uaagcgagua cccucaacug     1980

acagaacagc agcagguaca acuugcucag guucguuucc gggggugggg acgauuguca     2040

cagagacuca uuaauaggau caagacccca gugaguggcg augaggacca caaacugucc     2100

auuaacgaaa uccuauggca gacuaaugag aacuuuaugc aaauaauccg caacaaggau     2160

uaucuguuca aaaagauuau cgaggaacag uuugagaaug aaaccgcccu ccugaacaaa     2220

cagcggauug acgagcuugc aacaagcccc gcgaauaaga agggcaucug gcaagcuaua     2280

aaaauuguca aggaauugga gaaagugcug cagcagccug ccgagaacau cuucaucgaa     2340

uuugcaagau cugaugagga aucaaagagg aguaccccac gcgacaaauu cauugagaag     2400

gccuacgcuc agcucaaaaa ggaaacugac acauuuaauu uagagcaccu gaaggagcuu     2460

aaacaacgaa gcaagcagcu guccucucag cgguuguucc ucuauuucau ccagaacggg     2520

aaauguaugu acucgggaga acaucuggau auugagcguc uagacagcua cgaagucgau     2580

cacauacugc cccaauccua uaucaaggac aauucaauug agaaccucgc ccuggugaaa     2640

aagguugaaa aucagagaaa gaaagauagu cuuuugcuga acucuagcau cauuaaucag     2700

aacuacucca ggugggagca guuaaagaau gcaggucuca ucggcgagaa aaaguuucgc     2760

aaccugacgc ggaccaaaau aacugacaga gauaaggaag gguucaucgc uaggcaacuu     2820

guggagacac gacagauuac caagcaugua acacagcugc uacaacagga auauaaagac     2880

acuaccaagg ucuuugccau caaagcgacg cucgugucag aucugcgccg gaaguucgag     2940

uuuauuaaaa auagaaacgu uaacgacuac caccacgccc aggaugcuuu cuugguggca     3000

uuucugggaa ccaauaucac aagcaacuau ccgaagauag agauggaaua cuuauucaag     3060

ggcuaucagc auuaccucaa ugaggucggu aaaucugccg cuaagccuaa auuuacuuuc     3120

auuguggaaa accuguccaa gcaacaguac aauaguacca caggggaggu aaaauggaac     3180

cccgaagugg acaucgccaa acuuaagagg auccugaauu uuaaacagug caacauuguu     3240

cguaaggucg aggagcagag cggcgcauug uucaaagaaa cuauauaucc aguggaggaa     3300

ucuuccucaa agaccauccc ucucaaaaag caccuggaua cggcgauuua cggaggcuau     3360

acagccguga auuacgcuag uuaugcccua auccaauuca aaaaggggcg caagcugaaa     3420

cgggagauua ucggaauacc ccuugcaguc cagaccagaa ucgacaacag cgaaacuucg     3480

cuccagacau acauugcuga gcagaucaag uccgagguug aaauucugaa ugguaggauc     3540

uugaaauauc aacugauauc uaacaauggc aaccgauuau acauugccgg gccacgggag     3600

cgccauaaug caagacagcu caucgugagc gacgaagccg cuaaggugau cuggcugauu     3660

ucaaccaaac aggcgguaga ggccacuuuu cuuaagcacu acacccugga acauuuggag     3720

gcagucuucg aggaacucau caggaagcag gcugccgauu aucaaauauu ugagaaccug     3780

auuaaaaaga ucgaagugaa caaagccuac uucuauaguu guacauacaa ugagaagguu     3840

aaagugauug aggaacuacu gaagaucacg caggcuaacg caaccaaugg agaccuuaag     3900

cuccugaaaa ugacuaaccg ggagggccga uuggguuccg ucucuguggc cuuagauuuu     3960

aagauaauua aucagagcgu uacagggcug uaucaaucca ucgaagacua caacaauuga     4020


<210>  2980
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2980
augagccuga acaccaauaa gaaauacaac aucggccucg acauugggac aacuuccgug       60

ggaugggccg ucacggaugc ugacggucag aaacuucuga aagcaaagaa uaaaaacuug      120

uggggcguuu cuuuauucga ggccgggaag accgcggcug aaagaagggg auaucgguca      180

acacgccgac gucugaauca cagaaaguuu aggcuacggc uccuggagga uauguucgaa      240

aaagagauac uuaguaagga ccccagcuuu uucauccgcc ugaaagaagc cuuuuugucc      300

ccuaaggaug agcaaaaaca guucaagggc aacauucucu uuaaugacaa ggauuacacu      360

gacgcagauu auuacgagca guauaaaacc aucuaccauc ugagauauga ccugauuucu      420

cagcacaggc aauucgauau ccgggaagug uaccucgcca uacaucacuu aaucaaguac      480

cgcggucauu uuauuuauga ggaccagaca uucaccacug augggaacca gcugcagcac      540

cacaucaaag cuauuaucac aaugauaaau ucgauucuua accgaaucau uaucccagaa      600

accaucgaca uuaauguauu ugagaagacg uugcuggaca gaacuaugaa caggucaagc      660

aaagugaagu uccuaauaga gcucaccgga gaagauaaac aagacaagcc gcugcuuaag      720

gagcuguuua auuugaucgu cggccucaaa gcaaagcccg ccaguauuuu cgaacaggag      780

aaccuggcua caaucgugga aacuaugaau auguccaccg agcagguuca guuagaucug      840

cucacacuug ccgacgugcu ggcggaugag gaauacgacu ugcuacugac cgcacaaaag      900

auauauucug ccauuauccu cgaugagagc auggacggcu acgaauauuu cgcugaggcc      960

aagaaagaau ccuaccggaa gcaucaggag gagcuggucc uugugaagaa aaugcugaag     1020

ucaaacgcaa ucacgaacga ugaacgugcu aaauuugagu auuucuacac ugacuacauu     1080

ggggccaaga guagcaauua ugaagagucu aaaaucauua agaagggauu guccgcggcc     1140

uacgguaaau auucaaagga agaacgccuc uuuaaacaca uagagcuguu acuggcaaag     1200

gaaaacguac uaacaaccgu ugagcaugau cuccuggaaa agaauaucac uuucgcuagc     1260

cuuuugccuc ugcagagaag uucuaggaac gccgugauuc cauaccaagu ccacgagaag     1320

gagcucgugg cuauccugga aaaucaggcc acauauuacc ccuuucuucu ggagcagaag     1380

gacaacaucc acaaauugcu caccuuccgg auuccuuauu acgugggccc acuggcagac     1440

cagaaggauu ccgaauuugc cuggaugguc cgaaaacaag cugggaagau aacgcccuuc     1500

aauuuugagg aaaugguuga caucgaugcg agcucggagg cauucauuaa acgcaugacc     1560

aacaagugca cauacuuaau ccaugaggac gugauuccua aacacucuuu uuccuaugcc     1620

aaguucgaag uacugaauga gcuaaacaag aucagacucg auggaaaacc aauagacauc     1680

ccgcugaaga aaaggauuuu ugaaggccuu uucuuggaga agacuaaagu gacccauaca     1740

ucacugaaga aguggcucgc ugaacacgag cauaugacug ucaguguggu ucaggguacc     1800

cagaaagaga cagaguucgc caccagccug caggcauuuc accgguucgu gaagaucuuu     1860

gaucgcgaga cugucuccaa ucccgccaac gaagagaugu ucgagaaaau uaucuacugg     1920

ucuacggugu uugaagacaa gaaaauaaug agaaggaagu uaagcgagua uccucaacug     1980

acagaacagc agcagguaca acuugcucag guucguuucc gggggugggg acgauuguca     2040

cagagacuca uuaauaggau caagacccca gugaguggcg augaggacca caaacugucc     2100

auuaacgaaa uccuauggca gacuaaugag aacuuuaugc aaauaauccg caacaaggau     2160

uaccuguuca aaaagauuau cgaggaacag uuugagaaug aaaccgcccu ccugaacaaa     2220

cagcggauug acgagcuugc aacaagcccc gcgaauaaga agggcaucug gcaagcuaua     2280

aaaauuguca aggaauugga gaaagugcug cagcagccug ccgagaacau cuucaucgaa     2340

uuugcaagau cugaugagga aucaaagagg aguaccccac gcgacaaauu cauugagaag     2400

gccuaugcuc agcucaaaaa ggaaacugac acauuuaauu uagagcaucu gaaggagcuu     2460

aaacaacgaa gcaagcagcu guccucucag cgguuguucc ucuacuucau ccagaacggg     2520

aaauguaugu auucgggaga acaccuggau auugagcguc uagacagcua cgaagucgau     2580

cauauacugc cccaauccua caucaaggac aauucaauug agaaccucgc ccuggugaaa     2640

aagguugaaa aucagagaaa gaaagauagu cuuuugcuga acucuagcau cauuaaucag     2700

aacuauucca ggugggagca guuaaagaau gcaggucuca ucggcgagaa aaaguuucgc     2760

aaccugacgc ggaccaaaau aacugacaga gauaaggaag gguucaucgc uaggcaacuu     2820

guggagacac gacagauuac caagcacgua acacagcugc uacaacagga auacaaagac     2880

acuaccaagg ucuuugccau caaagcgacg cucgugucag aucugcgccg gaaguucgag     2940

uuuauuaaaa auagaaacgu uaacgacuau caccaugccc aggaugcuuu cuugguggca     3000

uuucugggaa ccaauaucac aagcaacuac ccgaagauag agauggaaua uuuauucaag     3060

ggcuaccagc acuaucucaa ugaggucggu aaaucugccg cuaagccuaa auuuacuuuc     3120

auuguggaaa accuguccaa gcaacaguac aauaguacca caggggaggu aaaauggaac     3180

cccgaagugg acaucgccaa acuuaagagg auccugaauu uuaaacagug caacauuguu     3240

cguaaggucg aggagcagag cggcgcauug uucaaagaaa cuauauacuc uguggaggaa     3300

uccucaagua agaccauccc acucaaaaag caucuggaua cggcgauuua uggaggcuac     3360

acagccguga auuaugcuag cuacgcccua auccaauuca aaaaggggcg caagcugaaa     3420

cgggagauua ucggaauacc ucuugcaguc cagaccagaa ucgacaacuc ggaaacuucc     3480

cuccagacau auauugcuga gcagaucaag ucugagguug aaauucugaa ugguaggauc     3540

uugaaauacc aacugauaag caacaauggc aaccgauuau auauugccgg gccccgggag     3600

cgccacaaug caagacagcu caucguguca gacgaagccg cuaaggugau cuggcugauu     3660

aguaccaaac aggcgguaga ggccacuuuu cuuaagcauu acacccugga acacuuggag     3720

gcagucuucg aggaacucau caggaagcag gcugccgauu accaaauauu ugagaaccug     3780

auuaaaaaga ucgaagugaa caaagccuau uucuacuccu guacauauaa ugagaagguu     3840

aaagugauug aggaacuacu gaagaucacg caggcuaacg caaccaaugg agaccuuaag     3900

cuccugaaaa ugacuaaccg ggagggccga uuggguucug ucagcguggc cuuagauuuu     3960

aagauaauua aucaguccgu uacagggcug uaccaaucaa ucgaagacua uaacaauuga     4020


<210>  2981
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2981
augaagaaag aguacaccau cggccuggac auugggacaa acagcguggg augguccguc       60

cucacugaug acuauagacu uguuucuaag aaaaugaagg uggccgguaa uacggaaaaa      120

ucaaguacca agaagaacuu cuggggcgua aggcuguuug augaggggca gacagcugaa      180

gcacggcgca gcaaacgaac ugcccguaga agguuggcgc ggcgcagaca aaggauauua      240

gagcugcaga agaucuucgc ucccgaaauu cuaaaaaucg acgagcacuu uuucgcccgg      300

cucaaugagu ccuuucuggu gcuugaugaa aagaaacagu cucgccaucc ugucuucgca      360

accauuaagc aggagaaguc guaccaccaa acauauccaa ccaucuacca ucugcgacag      420

gccuuggcug acucaagcga aaaagcagau auaagacucg uguaucuggc cauggcucau      480

cugcucaagu acaggggaca cuuuuuaauc gagggcgagc ugaacacuga aaauaguucc      540

guuacagaga ccuuccggca guuucuuucu acguauaacc agcaauucag cgaagccgac      600

gauaaacaga cugagaaguu ggacgaagcg guggauugcu ccuuugucuu caccgagaaa      660

augucaaaga cuaaaaaggc agagacacug cuaaaguacu uuccgcacga gaaaaguaau      720

gguuaccuca gccaguucau uaagcugaug guggggaacc agggaaauuu caaaaacgua      780

uuuggccuug aagaagccaa gcugcaauuc ucuaaagaga ccuaugaaga ggacuuggag      840

gaacuccugg agaagaucgg cgaugacuac auugacuuau uuguucaggc uaagaaugug      900

uaugaugccg uccugcucuc cgaaauccuu ucagacagca ccaaaaacac acgugcaaag      960

cugagugcug ggaugauacg cagauacgau gcccacaaag aggacuuggu gcuacugaag     1020

agguucguga aagaaaaucu ccccaagaag uaucgggcgu uuuucggaga uaacucuguc     1080

aaugguuacg ccggcuauau ugaggggcau gcaacgcagg aggacuuuua caaauucguu     1140

aagaaagaac ugacuggaau ccgaggcucc gagguguuuc uuacaaagau ugaacaagag     1200

aacuuccugc gcaaacagag aaccuuugau aacgguguaa ucccucacca gauccauuug     1260

acugagcuca gggcuauuau agccaaucag aagaagcacu acccauuccu gaaagaggaa     1320

caagaaaagu uagagagccu gcuaacauuu aaaauccccu auuacguggg gccucucgcu     1380

aagaaacagg aaaacucgcc auucgccugg cugauucgga agucugagga gaaaaucaag     1440

cccuggaauc uuccugaaau agucgacaug gagggauccg cagugcgcuu cauugaaaga     1500

augaucaaca ccgauaugua uaugccacac aauaagguuu ugccgaaaaa cucacugcuc     1560

uaccagaagu uuaguaucua uaaugagcug acgaaaguga gguaccagga cgaacguggc     1620

caaaugaacu auuucagcuc cauugagaag aaagagaucu uucaugaacu uuucgagaag     1680

aaucggaagg ucaccaaaaa ggaucugcag gaauuuuugu accucaaaua cgacauuaag     1740

cacgccgagc ugucuggcau agaaaaagcc uucaacgcga gcuauacaac uuaccaugac     1800

uuuuuaacca ugucagagaa uaagcgagag augaagcagu ggcuggaaga ucccgagcua     1860

gcaaguaugu ucgaagagau cauuaaaaca cucacugugu uugaggacag agaaaugauc     1920

aagaccaggc ugucccacca ugaggccaca cuugggaaac acaucauuaa gaaauugacc     1980

aagaagcacu auacuggaug gggucgccug agcaaagaac ucauacaggg cauccgggau     2040

aagcaaucua acaaaacgau ucuggacuac uuaaucaaug augacgauuu cccucaucac     2100

agaaacagga auuuuaugca gcugauuaac gacgauucac uuaguuucaa gaaagagauc     2160

aagaaagcuc agaugauaac agacaccgaa aacuuggagg agaucguaaa ggaacucacu     2220

gggagcccag ccauuaagaa aggaauccug cagucccuaa agauuguuga ugagaucgug     2280

ggcauaaugg guuaugaacc cgcaaauauu gucguggaga uggcccgcga aaaccaaacc     2340

acagggcgag gccugaaauc uucgcggccu cgucucaagg cucuggagga gagccuuaaa     2400

gacuuuggau cccaguugcu gaaggaauac ccaaccgaua auucaaguuu acagaaggac     2460

agacucuauc uguacuaccu ucaaaacggc agggacaugu auacuggggc uccccuggau     2520

auccaucgcu ugucugacua cgauauugac cacaucauac cgcggagcuu cacaacggau     2580

aauuccaucg acaacaaagu ccucguguca agcaaggaga auagacugaa aaaggaugac     2640

guuccuucug aaaaaguggu aaagaagaug agguccuucu gguaugaucu auacaguagc     2700

aaacugauuu cuaagcgaaa acucgacaac cugaccaaga ucaaacuuac ugaggaagau     2760

aaggcaggau uuauuaagcg ccaguugguc gagacacggc agaucaccaa acacguggcg     2820

gguauacugc aucacagauu caauaaggcu gaggacacaa acgaacccau uaggaaaguu     2880

cguaucauca cuuuaaaguc cgcccucgug ucacaguuuc gcaaucgguu cggcauuuau     2940

aaagucagag agaucaacga auaccaucac gcacaugaug ccuaucugaa ugggguggua     3000

gcucuugccc ugcuaaagaa guacccacaa cucgcaccug aguuugugua cggagaguau     3060

cugaaauuca acgcucacaa ggccaauaaa gcgaccguua agaaagaauu uuacaguaac     3120

auuaugaagu ucuuugagag cgacacgccc gucugugacg aaaacggcga gauauucugg     3180

gauaaaucga aguccaucgc ccaggugaag aaagugauua aucaccauca caugaacauc     3240

gucaagaaaa ccgaaauuca gaaggguggg uuuucuaaag agacaguuga gccaaagaag     3300

gacagcucaa aauugcugcc caggaagaau aacugggauc cugcuaaaua uggcggauua     3360

ggcaguccaa auguggcaua cacuguggcc uucaccuaug aaaaggggaa agcucgaaag     3420

cggacaaacg cccucgaggg aaucacuaua auggaacgcg aggcauucga acaguccccg     3480

guacuguuuc uuaagaauaa agguuacgag caagcggaga ucgaaaugaa gcugcccaaa     3540

uaugccuugu ucgagcucga aaacggcaga aagaggaugg ucgcuucuaa uaaagaggcc     3600

cagaaggcaa acagcuuucu gcuaccugaa caucugguga cccuucucua ccacgcuaag     3660

caguacgacg agauuuccca uaaagaguca uucgauuaug uuaaugaaca ccacaaggag     3720

uuuagcgaag uguucgcccg gguccuggag uuugcaggga aauacacguu ggccgagaag     3780

aacaucgaaa aacuggagaa gauuuauaaa gaaaaucaga cagacgauuu agcuaagcuc     3840

gcgaguucuu ucgugaaccu gaugcaauuu aaugccaugg gagcaccagc ugacuucaag     3900

uuuuucgaug uuaccauccc ccgaaaaaga uacacuuccc uuacagagau auggcagagc     3960

accauuaucc aucagucgau cacuggccug uaugaaaccc gcauuaggau ggguaaguga     4020


<210>  2982
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2982
augaagaaag aguacaccau cggccuggac auugggacaa acagcguggg augguccguc       60

cucacugaug acuauagacu uguuucuaag aaaaugaagg uggccgguaa uacggaaaaa      120

ucaaguacca agaagaacuu cuggggcgua aggcuguuug augaggggca gacagcugaa      180

gcacggcgca gcaaacgaac ugcccguaga agguuggcgc ggcgcagaca aaggauauua      240

gagcugcaga agaucuucgc ucccgaaauu cuaaaaaucg acgagcacuu uuucgcccgg      300

cucaaugagu ccuuucuggu gcuugaugaa aagaaacagu cucgccaucc ugucuucgca      360

accauuaagc aggagaaguc guaccaccaa acauauccaa ccaucuacca ucugcgacag      420

gccuuggcug acucaagcga aaaagcagau auaagacucg uguaucuggc cauggcucau      480

cugcucaagu acaggggaca cuuuuuaauc gagggcgagc ugaacacuga aaauaguucc      540

guuacagaga ccuuccggca guuucuuucu acguauaacc agcaauucag cgaagccgac      600

gauaaacaga cugagaaguu ggacgaagcg guggauugcu ccuuugucuu caccgagaaa      660

augucaaaga cuaaaaaggc agagacacug cuaaaguacu uuccgcacga gaaaaguaau      720

gguuaccuca gccaguucau uaagcugaug guggggaacc agggaaauuu caaaaacgua      780

uuuggccuug aagaagaggc caagcugcaa uucucuaaag aaaccuauga ggaggacuug      840

gaggaacucc uggaaaagau cggcgaugac uacauugacu uauuuguuca ggcuaagaau      900

guguaugaug ccguccugcu cuccgagauc cuuucagaca gcaccaaaaa cacacgugca      960

aagcugagug cugggaugau acgcagauac gaugcccaca aagaagacuu ggugcuacug     1020

aagagguucg ugaaagagaa ucuccccaag aaguaucggg cguuuuucgg agauaacucu     1080

gucaaugguu acgccggcua uauugagggg caugcaacgc aggaagacuu uuacaaauuc     1140

guuaagaaag agcugacugg aauccgaggc uccgaagugu uucuuacaaa gauugagcaa     1200

gagaacuucc ugcgcaaaca gagaaccuuu gauaacggug uaaucccuca ccagauccau     1260

uugacugaac ucagggcuau uauagccaau cagaagaagc acuacccauu ccugaaagag     1320

gaacaagaga aguuagaaag ccugcuaaca uuuaaaaucc ccuauuacgu ggggccucuc     1380

gcuaagaaac aggagaacuc gccauucgcc uggcugauuc ggaagucuga ggagaaaauc     1440

aagcccugga aucuuccuga aauagucgac auggaaggau ccgcagugcg cuucauugag     1500

agaaugauca acaccgauau guauaugcca cacaauaagg uuuugccgaa aaacucacug     1560

cucuaccaga aguuuaguau cuauaaugaa cugacgaaag ugagguacca ggacgagcgu     1620

ggccaaauga acuauuucag cuccauugag aagaaagaaa ucuuucauga gcuuuucgaa     1680

aagaaucgga aggucaccaa aaaggaucug caggaguuuu uguaccucaa auacgacauu     1740

aagcacgccg aacugucugg cauagagaaa gccuucaacg cgagcuauac aacuuaccau     1800

gacuuuuuaa ccaugucaga gaauaagcga gaaaugaagc aguggcugga ggaucccgaa     1860

cuagcaagua uguucgagga gaucauuaaa acacucacug uguuugaaga cagagagaug     1920

aucaagacca ggcuguccca ccaugaagcc acacuuggga aacacaucau uaagaaauug     1980

accaagaagc acuauacugg auggggucgc cugagcaaag agcucauaca gggcauccgg     2040

gauaagcaau cuaacaaaac gauucuggac uacuuaauca augaugacga uuucccucau     2100

cacagaaaca ggaauuuuau gcagcugauu aacgacgauu cacuuaguuu caagaaagaa     2160

aucaagaaag cucagaugau aacagacacc gagaacuugg aggaaaucgu aaaggagcuc     2220

acugggagcc cagccauuaa gaaaggaauc cugcaguccc uaaagauugu ugaugaaauc     2280

gugggcauaa uggguuauga gcccgcaaau auugucgugg aaauggcccg cgagaaccaa     2340

accacagggc gaggccugaa aucuucgcgg ccucgucuca aggcucugga ggaaagccuu     2400

aaagacuuug gaucccaguu gcugaaggag uacccaaccg auaauucaag uuuacagaag     2460

gacagacucu aucuguacua ccuucaaaac ggcagggaca uguauacugg ggcuccccug     2520

gauauccauc gcuugucuga cuacgauauu gaccacauca uaccgcggag cuucacaacg     2580

gauaauucca ucgacaacaa aguccucgug ucaagcaagg aaaauagacu gaaaaaggau     2640

gacguuccuu cugagaaagu gguaaagaag augagguccu ucugguauga ucuauacagu     2700

agcaaacuga uuucuaagcg aaaacucgac aaccugacca agaucaaacu uacugaagag     2760

gauaaggcag gauuuauuaa gcgccaguug gucgagacac ggcagaucac caaacacgug     2820

gcggguauac ugcaucacag auucaauaag gcugaagaca caaacgagcc cauuaggaaa     2880

guucguauca ucacuuuaaa guccgcccuc gugucacagu uucgcaaucg guucggcauu     2940

uauaaaguca gagaaaucaa cgaguaccau cacgcacaug augccuaucu gaauggggug     3000

guagcucuug cccugcuaaa gaaguaccca caacucgcac cugaguuugu guacggagaa     3060

uaucugaaau ucaacgcuca caaggccaau aaagcgaccg uuaagaaaga guuuuacagu     3120

aacauuauga aguucuuuga aagcgacacg cccgucugug acgagaacgg cgaaauauuc     3180

ugggauaaau cgaaguccau cgcccaggug aagaaaguga uuaaucacca ucacaugaac     3240

aucgucaaga aaaccgagau ucagaagggu ggguuuucua aagagacagu ugaaccaaag     3300

aaggacagcu caaaauugcu gcccaggaag aauaacuggg auccugcuaa auauggcgga     3360

uuaggcaguc caaauguggc auacacugug gccuucaccu augagaaggg gaaagcucga     3420

aagcggacaa acgcccucga aggaaucacu auaauggagc gcgaagcauu cgagcagucc     3480

ccgguacugu uucuuaagaa uaaagguuac gagcaagcgg aaaucgagau gaagcugccc     3540

aaauaugccu uguucgaacu cgagaacggc agaaagagga uggucgcuuc uaauaaagaa     3600

gcccagaagg caaacagcuu ucugcuaccu gagcaucugg ugacccuucu cuaccacgcu     3660

aagcaguacg acgagauuuc ccauaaagaa ucauucgauu auguuaauga gcaccacaag     3720

gaauuuagcg agguguucgc ccggguccug gaguuugcag ggaaauacac guuggccgaa     3780

aagaacaucg agaaacugga aaagauuuau aaagagaauc agacagacga uuuagcuaag     3840

cucgcgaguu cuuucgugaa ccugaugcaa uuuaaugcca ugggagcacc agcugacuuc     3900

aaguuuuucg auguuaccau cccccgaaaa agauacacuu cccuuacaga aauauggcag     3960

agcaccauua uccaucaguc gaucacuggc cuguaugaga cccgcauuag gauggguaag     4020

uga                                                                   4023


<210>  2983
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2983
augaagaccg acuacuucau gggccuggau auggggacag gaagccucgg uugggccgug       60

acugacuccu cuuaucaccu uugcagagcu cauggcaaag aucugugggg agucagguug      120

uuugagacgg cacagaccgc cgaagaacgg cgcguuuucc gauguaaccg uagaagguua      180

gaccggcgca agagaaggau ccaacugcua caggauauuu uugcgcccga aauauacaaa      240

aucgacccug gguucuuucu ccggcugaag gagucacgcu aucuuccaga agauaaaaag      300

aaucuggagg gcaagacacc ggaguugccc uacgcucucu ucguggacaa aaaguauacu      360

gauaaagacu uucacaagga auacccuacc auuuaucauc ugcgacugag acucaugcag      420

gauaggacac cagccgacgu acgguuagug uaccuggcac uucaccauuu gaucaaacac      480

cguggucacu uccuguuugc cgggauugag gcugauaagg ucaccgacuu cagugcagcc      540

uuuagcgagu uccuacgcau cgugcaggag caagagcucg acauauccuu uacucccgau      600

gaagagucuu cgcagaucau uaaggaaaca cugcagaaca ccaauuucac gagaagcaaa      660

aagucaacug agcuuaucaa aggacugaag gcuaguaccg gcugcgaaaa agccuugcuc      720

aagcugauuu ccggcuguaa aguuaaguua ucugcggugu ucgacgaucc ugggugcgac      780

gcauacgaga agccacaggu cuguuuugcc gagagcuccu augaagauua cgcugccauc      840

cuggaggacg aacucgagga uagguucaua gugauugcag cugccaaagc gcuuuaugac      900

uggucaguac ugguuaacau cuugggagaa gccaguacag ugagcgaggc aaaggucggu      960

auuuacgaga aacaucggcg agaucuaaag uaucugaaac accucgugaa gacuaaucug     1020

acccgcgaag acuaccaaga ucuuuuugug aaggcuggcg ccaaaaagaa caauuauugc     1080

gcuuacaucg ggaugaccaa aaagaacaau aaaaaggucg uucuggagga aaagagaugu     1140

acaaaagagg acuucuacgc cuauuugaag aaagaaaucu acucuaaggu gcaggagaac     1200

cccgaggcag aauaucuccu ggaggaauua gagaaaggaa cguuucugcc uaggcaggua     1260

uccaaggaca auggcgugau uccauaccag cuacaauucc ucgagcugca gaagauacuu     1320

aaaaacgccg ucgaauauuu gccguuucug aaggaggaau cagagaaaau ccagcaaauu     1380

augacuuucc ggauccccua cuaugugggu ccucucaaug ggaagaaaga aggacagaac     1440

acauuugcuu gggcgguucg caagaauaac gagccagugu accccuggaa cuucgaggaa     1500

gucauagaua ccgaggcaag cgccgaacag uuuauuagaa ggaugacuaa uaaaugcaca     1560

uaccugccug gcgaggacgu gcuuccaaag gaaagucugu uguauucuaa guucauggua     1620

cucaacgagc ugaauaacuu acguaucaau ggcaccaaaa ucuccguuga gcugaagcag     1680

gaacuauacg agaaacucuu ucaacgguau cgaaagguga gcgcuaaaaa gcugagagau     1740

uaccuuguca ggaacgggau uacggaaaag gacgccgaga ucucgggaau ugauggugac     1800

uucaaaggca ccuugcgcgc auaucaugau uucaaggaaa aacugacagg gguggagcuc     1860

acugaggccg aaaaggagac cauaauccug aauauuacau uauuuggaga agacaaaguc     1920

cugcuuaaga agcggaugca gagauugcuc cccaaccuga cugagggcca gaaaaaggcu     1980

aucgugaccc uaaauuacuc ugguuggggg aggcuguccc gcgaguuccu cgaagagguu     2040

caggccccga acccugaauc aggcgagauc cugaguauua uacgagcacu uugggaaaca     2100

aaugcgaacu ugaugcaacu guuaagcaau caguauggau accaggagau ccucgagcag     2160

augcgggcug auuggaccgg ccacaaacug gacuaccaaa cuguggaaga acuuuaugua     2220

ucccccgccg ucaagcguca gauuuggcag acgcugcaag ugguuaaaga auugaagaag     2280

gugaugggag gggagccaaa aagagucuuu cucgaagugg caagggagaa gauggagucu     2340

ggucgcacag uaagccggaa aaagcaccug aaagaucuau accugucaug uaagagugaa     2400

gagagaaacu ggcucgccga acuggacagg caugaggaac acuccuaucg aagcgaucgc     2460

cuuuucuugu acuauaccca gaaaggccgg ugcauguacu gugggaagcc uaucgaccug     2520

ggacauuuau gggacgugaa uauguaugau auugaccaca ucuaccccca gucuaagguu     2580

auggaugacu caauaagaaa caggguccuc acuugccgua ccugcaacgc ugagaaaaca     2640

gaucauuacc cacugaaggc cgagauccgc cagaaaaugc ggcccuuuug ggacagucuu     2700

aaggcaggca auuucauuga agaggaaaaa uggaagagac ugaucaggag cgaggaguuu     2760

accuccacug aacuagcugg uuucauugag cgacaacucg uggaaacacg gcagucuacg     2820

aaggccgugg cggauauccu gaaacaggcc augccugaga ccgaaauagu cuauguuaag     2880

gcuaaaaacg ugucggcauu ucgccaggac uucaauuuug ugaagaacag agauguaaau     2940

gacuuccacc acgccaaaga ugcuuacuug aacauugucg uggggaauac uuauuuuguu     3000

aaguucacaa agaacgccag cugguucauc gagaggaauc caggccgguc cuacaaccug     3060

aaacgaaugu uuaccucaga cuaugaugug augaguaaug gagagauugc auggaaggcg     3120

ggcaacucug ggacaaucac uaccgucaga aaaaugauga agaaaaauag cguguuauuc     3180

acgcgcaggu ccuuugaagu uucaggaggu cucuucgacc aacagcugau gaagaagggc     3240

aaaggacagg uguacguaaa gagcggggac gagcgucuuc ggucuauaga aaaauauggc     3300

gguuacaaca aggccaccgg ggcuuacuuu augcuggucg aguccgaagg caaaaaggga     3360

gugaagaaaa gaacaaucga guucgugccg uuguauagga aggccgagcu cgaagcaagu     3420

gaggagaaaa ugcuggagua ccuacgcacu gaaauuggcc ugaaggagcc ccauguccuu     3480

cuccacaaaa ucaagaccga uacacuguuu caggcugacg gguucaaaau gcauuugagc     3540

ggacgaacug gcaagcaacu gguuuuuaag ggugccaauc aguucuuagu ggcagaggau     3600

uaugaaagac uccugaaaaa gguagggaaa gugaccgagc agcuuaagac gaacaaaaac     3660

uacaagauua augacuauga uggaaucaca gaagagggcc ugaaggccuu guacucugag     3720

uuucucucca aacugcagaa ggcuccuuau gguaaccggc uaggcaccca agcggaaacu     3780

cuggagaaug ggaaagaaaa guucgacaaa cuuucacucg agaacaagug ucagguccug     3840

agugaaauau ugcacuuauu ccagugcaau agcucggccg cagaucuguc caagcucgga     3900

ggcccagggc acggaggcau ucuggugcuu ucuaacgaga ucacaaaaug uaauaagauc     3960

agcauuaucc aucaaucacc cgcugguauu uacgagcagg aaauagaccu gaaaaccuug     4020

uga                                                                   4023


<210>  2984
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2984
auggagcagg aauacuaucu gggccucgac auggggaccg gaagcguggg uugggccguc       60

acagauuccg aguaccacgu ucuuagaaag cauggcaaag cucugugggg agugagguug      120

uucgaaucug caucaacugc cgaggaacgg cgcauguuuc gaacgagucg uagaagguua      180

gaccggcgca acuggagaau cgagauucug caagagauau ucgcggaaga gaucagcaag      240

aaagaucccg gguuuuuccu aaggaugaag gaauccaaau auuacccuga ggacaagcgg      300

gauauuaaug gcaacugccc agagcucccg uaugcucugu uuguagacga ugacuucacc      360

gauaaggacu accacaaaaa guuucccaca aucuaucauc uucgcaaaau gcugaugaau      420

acugaagaga ccccugauau ucgauuggug uaccucgcca uccaccauau gaugaagcac      480

agaggucacu uccugcuguc uggggacaua aacgaaauca aagaguuugg aacaaccuuc      540

ucgaagcucu uagaaaauau uaagaacgag gagcuggauu ggaaucuuga auugggcaaa      600

gaggaauacg cagucgugga gucaauccug aaggacaaca ugcuaaauag gagcacuaaa      660

aagacacggc ucauuaaagc ccugaaggcu aaaaguaucu gugaaaaggc aguucuuaac      720

cuguuggccg gcgggaccgu gaagcucucc gacauauuug gacuggagga guuaaaugaa      780

acggagcguc caaaaauuuc uuucgcugau aacgguuaug acgauuacau cggcgaaguc      840

gagaaugaac ugggggagca guuuuauauu aucgagacug ccaaggcggu guacgacugg      900

gcaguacucg uugaaauccu uggaaaauau accagcauuu ccgaggccaa gguggcuaca      960

uacgaaaaac auaagucaga ucugcaguuc uugaagaaaa uaguccgcaa guaucuaacu     1020

aaagaggagu acaaggacau cuucgugagu accagcgaua aacugaagaa cuacucugcc     1080

uauauuggca ugacaaagau caaugguaaa aagguggacc uccaguccaa aagaugcuca     1140

aaggaagagu uuuacgauuu cauuaaaaag aacguccuga agaaacuuga agggcaaccc     1200

gaguaugaau accugaagga ggaguuggaa agggagaccu uucucccuaa acagguuaac     1260

cgggacaaug gagugauacc auaucagauc caccuguacg aauuaaagaa aauccugggc     1320

aaccuacgag auaagauuga ccucaucaaa gagaaugaag acaagcuggu acagcuuuuc     1380

gaguuucgca uucccuauua cgugggcccu uugaacaaga uagaugacgg gaaagaggga     1440

aaguucacgu gggcagucag aaaaagcaau gaaaagaucu acccauggaa cuuugagaau     1500

gugguugaua uugaagcuag ugccgagaaa uucaucaggc ggaugacuaa caaguguaca     1560

uaucugaugg gugaagacgu gcucccgaag gauucucugc uuuacuccaa auauaugguc     1620

cugaaugagu ugaacaaugu gaagcucgac ggcgagaaac ugagcguaga auuaaagcaa     1680

cgccuguaca ccgauguguu uugcaaauau agaaagguua cugucaagaa aaucaagaac     1740

uaccuaaaau gugaggggau uauaucggga aauguggaaa ucacaggcau ugacggugau     1800

uucaaggcgu cucucaccgc cuaucaugac uuuaaagaga uccugacggg gaccgagcuu     1860

gcaaagaagg auaaagaaaa cauuaucaca aauauagucu uguucggcga cgauaagaaa     1920

cugcucaaga aaaggcugaa ccguuuauac ccccagauca cuccuaacca gcugaagaaa     1980

auuugcgcuc uuuccuacac cggauggggc cgguuuucaa agaaguucuu ggaggaaauc     2040

acagccccag accccgagac uggggaagug uggaauauua ucaccgcucu cugggagagu     2100

aacaauaacc ugaugcagcu acugagcaau gaguaucgau ucauggaaga gguugaaaca     2160

uacaacaugg gaaaacaaac caagacucuc uccuaugaga cgguggaaaa uauguacgua     2220

ucuccuagcg ucaaaagaca gauauggcag acacugaaga uugugaaaga gcuugagaag     2280

guuaugaagg aaucaccaaa aaggguguuu aucgagaugg cccgcgaaaa gcaagagagu     2340

aaacggaccg aauccagaaa gaaacaguug auugaccugu auaaggcaug uaagaacgag     2400

gagaaagauu gggucaagga auuaggugac caggaggaac agaaacucag gagcgauaag     2460

cuguaccuuu auuacacuca aaaaggccgc ugcauguacu cuggggaggu gaucgagcug     2520

aaggacuugu gggauaauac caaguaugac auagaucaca ucuaccccca gucaaaaaca     2580

auggacgaua gucucaacaa ucgaguacug gugaagaaaa aguauaacgc caccaaaagc     2640

gacaaguacc cgcuaaauga aaacauucgg cacgagcgua agggauucug gaaaucccug     2700

cucgauggcg guuuuaucuc uaaggaaaaa uaugagagac ugauuaggaa uacugaacuu     2760

ucgccugagg aguuggcugg guucaucgaa cgccagauag uugagacacg gcagagcacg     2820

aaggcggucg cagaaauucu gaaacaagug uuucccgagu ccgaaaucgu guacgucaag     2880

gccggcaccg uuucaagauu caggaaagac uuugaguuac ucaaggugcg agaggugaac     2940

gaucugcauc acgcuaagga cgccuaucuu aacaucguag ucggaaauag uuacuacgug     3000

aaauucacua agaacgcauc uugguuuauu aaagaaaauc caggccgcac auauaaccug     3060

aagaaaaugu ucaccagcgg guggaauauc gagcggaacg gagaaguugc cugggaggug     3120

gguaagaagg gcacaauugu cacugugaaa cagauaauga auaagaacaa uauccuaguu     3180

accagacagg ugcaugaagc uaaaggggga cucuuugacc agcaaauuau gaagaaaggc     3240

aagggucaga ucgccauuaa ggagacggau gagaggcugg cauccaucga aaaauacggg     3300

ggcuauaaca aggcggcugg agccuacuuc auguugguag agucaaaaga caagaaaggc     3360

aagaccauac guacaaucga auuuauuccu cuguauuuaa agaauaaaau cgagagcgau     3420

gagucuauug cacucaacuu ccuggaaaag gggcgcggac uuaaagagcc caagauccug     3480

uugaaaaaga uaaaaauuga cacucucuuc gaugucgacg gcuuuaagau guggcugucc     3540

ggucggaccg gggauagacu acuguucaag ugugccaauc agcuuauccu cgacgagaaa     3600

aucauuguga caaugaagaa aauagugaag uuuauccaga ggcgacaaga aaaccgggaa     3660

cugaaauuga gugauaagga cggaauugau aaugaggucc ugauggagau cuacaacacu     3720

uucguugaca aguuagaaaa caccguguau cgcauuagac ucagcgagca ggcuaaaacg     3780

cugaucgaca agcagaaaga auuugagagg cuuucucugg aagauaaguc cucaacauug     3840

uucgagauac uccacaucuu ucaaugccag aguagcgccg caaaucugaa aaugauuggc     3900

gguccaggca aggcugggau ccuaguaaug aacaauaaca uuucgaagug uaauaaaauc     3960

uccauaauua accagucucc caccggaauc uucgagaaug aaaucgaccu gcuuaagauu     4020

uga                                                                   4023


<210>  2985
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2985
augggcgagg aauacuuccu ggggcucgac augggaaccg guagccuugg cugggccgug       60

acagaugaca aguaucagau ccugagaagg cacgggaacg cuuugugggg aguccgguua      120

uuugagacug caaauacggc cgaagaacgc cgaauguucc guaccaacag aaggcggcug      180

gaucgccaag gcuggagaau uaaaauacua caggaacucu uugcggagga gaucugcagg      240

guugaccccg guuucuaccu gcggaugaag gaauccaaau auuacccuga ggauaagcgc      300

gacgcuaaug gggaaugucc agcccuuccg uaugcacugu uugccgaugc ucaguacaca      360

gacaaagauu aucaucgaga guuccccacu auuuaccacu ugagaaagau gcucauggag      420

accgaaacaa ccccugacau caggcuggug uaccuggcau uucaucacau gaugaagcau      480

cggggacacu uccucuuauc uggcgauauu ucacagguaa cugaguuucg uaacacauuc      540

cugcaaauga ucgccaauau aaaaagugaa gaacuugacu uuagcuugga aaucgaggag      600

acccagaugg gccagauuga agcugugcug aaggaucaca aacuaacgcg cuccgagaag      660

aaaagaaggc ucacucagau ccugaagcca gccaccgcgu gcgaaaaggc aauuuucaca      720

cuucugucug ggggaacugu caaauugucg gacaucuuug guaaggccga gcucgacgaa      780

ggcgagcggc ccaaaauauc auucgcugau aacggguaug acgauuacag cgccagugug      840

gaggcagacc ugggagaaca auauuacauu aucgaguccg cuaaggccgu uuaugauugg      900

ucuguguuaa gcgacauucu gggcgauucc gucucaauca gugacgcgaa agugaagcuc      960

uacgaaaaac auaagaagga ucuugccuau cugaaaaagg uaguucgaga gaauuugccu     1020

cgcgaagugu acaaccaggu cuucgugaau accgacgaga aacuauguaa cuacugcgca     1080

uauaucggua uggcuaagaa aaaugggaga aagguggccc uggagggaaa gagguguaca     1140

cgggaagagu uugagagcuu ccuccgcaaa aagauuguca acaaaauaga ggauaaggag     1200

auccaggaau accugucuga ggaaauugag aaaggcaccu uucuuccaag acagguuacg     1260

aaggacaaug gcgugauccc cuaucaaaua caccuguacg aauugaggaa gauuaucgac     1320

aaccucuccc cucguaugcc auucauccgg gauaaugggg acaaaauuau ccagcuguuu     1380

acuuuccgaa uaccguauua cguaggaccc uuagguaagg gcgagggguc agagaaauuu     1440

agcugggcug ugagaaggac agaugaagcc auuuauccuu ggaacuucaa ggacgucauc     1500

gauguggagg caagugccga aaaauuuauu ugccgcauga ccaacaagug uacuuaccug     1560

uacggagagg acguucuacc aaaggauucu ccccucuauu ccaaauucau ggugcugaau     1620

gaacuuaaca auuugaagcu ggacggccag agcgucucgg uggagcucaa acaacggauc     1680

uaccaggagc uguuuugcca uacaagaaag guaacccaga aaaagcuggu uucuuaucuu     1740

gugagggaag guaucacggg gaaaaacguc gagauuuccg gcauagaugg agacuucaag     1800

gcuucauuga ccgcguacca cgauuuuaag gaaaaacuca caggcgugga gcugagucag     1860

caagaacagg aggagaucgu ccgcaauauu guguuauucg gggaagacaa gaaacugcua     1920

aagcagcgac ucgagggacg guucccucag cugacucaaa aacagaucag cguuauugca     1980

acccuuuccu auaaggguug gggccguuug ucuagagaau uucuggagaa caucacagug     2040

ccagcccccg ggacuggaga gguauggaau auaaucaccg cucucuggga aacaaacgau     2100

aaucugaugc aguuacugua cggcgaguau gguuuccaga ccgaaauuga guguuuuaac     2160

gccaaggaga aaaagaaaag ccuuucauac gagacuaucg aggaaaguua cgucuccccg     2220

agcgugaaga ggcaaauuug gcagacguug cagguuguga aagagcucaa gaaggucaug     2280

cgcggggacc cuaaacgggu guucaucgaa auggcaagag agaaucagga aucuaagagg     2340

acagagucac gaaagaagac ccugcuagac cuguauaaaa acugcaagaa ggaggaaccc     2400

gaguggauag ccgaacucac ugagcaccug gaaaaacuug augacaguca auugcgcaag     2460

gauaaacugu uuuuauacua uacccagaag ggccggugua uguacagcgg agagagaauu     2520

gagcucgaag accuguggga uaauacaagg uaugacaucg aucauauuua cccacagucc     2580

aaaaccaugg acgauucugu agacaaccgu gugcuuguua agaaagagua uaaugcuaag     2640

aagacugaug ucuacccuau cgccgcagac auacgcggca aaaugucgcc cuucuggaag     2700

aaacuguugg auaacaagcu caucagcaaa aagaaguacg accggcuggu gagaacagaa     2760

gaacuaacgc cagaggaacu ggcgggguuu auugacaggc aacucgugga gacccgacag     2820

uccacuaaag ucguugcuga aauccugaag gaggccuucc ccgauacaga aauuguguau     2880

gugaaagcag gaaacguauc aaauuuucgg cacauguucg acaucgucaa ggugcgcgag     2940

cuuaacgauu ugcaucacgc caaagacgcu uaccugaaua uaguuguggg uaacgccuau     3000

uacgucaagu uuaauaagaa cgcagcuugg uucauuaaag agaauccugg cagaaguuau     3060

aacuuaaaga ggauguuuga uuacgacauc cggcgaaaua acgaaguggc cuggcucgcg     3120

gggucuaccg gaacaaucag ccagguuaaa aauauggugu ccaagaacca uauucuggua     3180

acuagaaaau cauaugaggu caccggcggu cuuuucgauc agcaacugau gaagaagggg     3240

aaaggccagg ugccagugaa gacgagcgac gaacgccuag gaaauaucga gaaauacggc     3300

ggguacaaca aggccucugg agcuuauuuc augcucguca aauccaagaa uaagaaaggc     3360

gaggagcaga ggaccauuga guacguuccg auauaucuga agcacuacau cgaagagagu     3420

aaagaaaacg cauuggauua ucugaagaac gagcguaaau uaaaugaacc cgagauucuc     3480

cuggacaaga ucaaaaagga uacacuuuuu cgggugaacg guuucuacau gugccugagc     3540

aagagaacug ggaaacaguu ggccuuuaag ggagcuaaug agcuccugcu accugaacca     3600

gagacccugc uucucaaaaa gauuacaaaa uauauccaaa ggcgcaagga aaacaaggac     3660

cugaaauuga cuaagcagga caauauaacc gagaaagagc uguuagaacu cuacgaugua     3720

uucacggaga agcugaacca cucuguguac gccgucugcc uuggcacaca ggguaaaacc     3780

cuggugcaga agaagcccgc auuuguugcg uugccugugg aagagaaaug uaucguccuc     3840

uccgaaauuc ugcaucuauu ccaaugccag ucaggcagug ccaaucugaa acuuaucggg     3900

ggaccagcua gcgccggcau ucucuaucug aacucgaaua ucuccgacgc aggggagaua     3960

uugauuauca accagucucc cagcggaauc uuugagaaac gaauugauuu acugagacuc     4020

uga                                                                   4023


<210>  2986
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2986
augaagaaag aguacaccau cggccuggac auugggacaa acagcguggg augguccguc       60

cucacugaug acuauagacu uguuucuaag aaaaugaagg uggccgguaa uacggaaaaa      120

ucaaguacca agaagaacuu cuggggcgua aggcuguuug augaggggca gacagcugaa      180

gcacggcgca gcaaacgaac ugcccguaga agguuggcgc ggcgcagaca aaggauauua      240

gagcugcaga agaucuucgc ucccgaaauu cuaaaaaucg acgagcacuu uuucgcccgg      300

cucaaugagu ccuuucuggu gccugaugaa aagaaacagu cucgccaucc agucuucgca      360

accauuaagc aggagaaguc guaccaccaa acauauccga ccaucuacca ucuucgacag      420

gcccuggcug acucaagcga aaaagcagau auaagauugg uguaucucgc cauggcucau      480

cugcugaagu acaggggaca cuuucucauc gagggcgagu uaaacacuga aaauaguucc      540

guuacagaga ccuuccggca guuucugucu acguauaacc agcaauucag cgaagccggu      600

gacaaacaga cugagaaacu ugaugaagcg guggacugcu ccuuugucuu caccgagaaa      660

augucaaaga caaagaaggc agagacuuug cugaaguacu uuccccacga aaagaguaau      720

ggguaccuaa gccaguucau uaagcucaug gugggaaacc agggcaauuu caaaaacgua      780

uuuggccugg aggaagaggc caaacuucaa uucucuaaag aaaccuauga ggaggaucug      840

gaagaguugc ucgaaaagau cggggacgau uacauugacc uguuuguuca ggcuaagaau      900

guguaugacg ccgucuuacu guccgagauc cucucagaua gcacaaagaa cacccgugca      960

aaacuuagug cuggaaugau acgcagauac gacgcccaca aagaagaucu gguguugcua     1020

aagagguucg ugaaagagaa ucugccuaag aaguaucggg cguuuuucgg ugacaacucu     1080

gucaauggcu acgccgggua uauugaggga caugcaacgc aggaggcuuu uuacaaauuc     1140

guuaagaaag aacucacugg cauccgaggu uccgaagugu uucugacaaa gauugagcaa     1200

gagaacuucc uucgcaaaca gagaaccuuu gauaacgggg uaaucccaca ccagauccau     1260

cugagcgaau ugagggccau uauagcuaau cagaagaagc acuaccccuu ccucaaagag     1320

gaacaagaga agcuggaauc guuacugacu uuuaaaaucc cuuauuacgu gggaccacua     1380

gccaagaaac aggagaacuc ucccuucgca uggcucauuc ggaaguccga ggaaaagauc     1440

aagccuugga aucugccaga gauagucgac auggaaggcu cagccgugcg cuucauugag     1500

agaaugauca acacagauau guauaugccg cacaauaagg uucuucccaa aaacaguuug     1560

cuguaccaga aguuuagcau cuauaaugaa cucaccaaag ugagguacca ggacgagcgu     1620

ggccaaauga acuauuucuc cucuauugag aagaaagaaa ucuuucauga gcuguucgaa     1680

aagaaucgga aggucacgaa aaaggaucuu caggaguuuc uguacuugaa auacgacauu     1740

aagcacgcug aacucagcgg gauagagaaa gcguucaacg caucauauac cacauaccau     1800

gauuuucuga cuaugaguga gaauaagcga gaaaugaagc agugguuaga ggacccugaa     1860

cuggccucca uguucgagga gaucauuaaa acccuaacag uguuugaaga cagagagaug     1920

aucaagacua ggcucagcca ccaugaagcu acccugggaa aacacaucau uaagaaacuu     1980

acaaagaagc acuauaccgg uuggggccgc uugucuaaag agcugauaca ggggauccgg     2040

gauaagcaau caaacaaaac uauucucgac uaccugauca augaugacga uuucccacau     2100

cacagaaaca ggaauuuuau gcaguuaauu aacgacgaua gucugagcuu caagaaagaa     2160

aucaagaaag cccagaugau aacggacaca gagaaccuug aggaaaucgu aaaggaguug     2220

accggauccc ccgcaauuaa gaaaggcauc cuccagucuc ugaagauugu ugaugaaauc     2280

guggguauaa ugggguauga gccugccaau auugucgugg aaauggcucg cgagaaccaa     2340

acuaccggcc gaggacuaaa aucgagccgg ccacgucuga aggcccucga ggaaucccug     2400

aaagacuuug gcucacagcu uuugaaggag uaccccacag auaauaguuc ucugcagaag     2460

gacagauuau aucucuacua ccugcaaaac gggagggaua uguauaccgg agcaccgcuu     2520

gacauccauc gccugagcga cuacgauauu gaccacauca uaccucgguc cuucacuaca     2580

gauaauucaa ucgacaacaa agucuuggug agcucuaagg aaaauagacu caaaaaggau     2640

gacguucccu ccgagaaagu gguaaagaag augaggaguu ucugguauga ucuguacagc     2700

ucuaaacuaa uuuccaagcg aaaacuggac aaccucacga agaucaaacu gaccgaagag     2760

gauaaggcgg guuuuauuaa gcgccagcuu gucgagacuc ggcagaucac aaaacacgug     2820

gcuggcauau ugcaucacag auucaauaag gccgaagaca ccaacgaucc aauuaggaaa     2880

guucguauca ucacacugaa gucagcauua gugagucagu uucgcaaucg guucgggauu     2940

uauaaaguca gagagaucaa cgaauaccau cacgcccaug acgcuuaucu caauggagug     3000

guagcccugg cacuucugaa gaaguacccu caacuagcuc ccgaguuugu guacggcgag     3060

uaucucaaau ucaacgccca caaggcgaau aaagccacug uuaagaaaga auuuuacagc     3120

aacauuauga aguucuuuga gucggauacc ccagucugug acgaaaacgg ugagauauuc     3180

ugggacaaau ccaagucuau cgcucaggug aagaaaguga uuaaucacca ucacaugaac     3240

aucgucaaga aaacggaaau ucagaaaggg ggcuuuagca aagagaccgu ugagcccaag     3300

aaggauucaa guaaacuguu gccuaggaag aauaacuggg acccagcaaa auauggaggc     3360

cugggguccc cgaauguggc cuacacagug gcuuucacuu augaaaaggg aaaagcccga     3420

aagcggacca acgcauuaga ggguaucaca auaauggaac gcgaggcguu cgaacagucu     3480

cccguacucu uucugaagaa uaaaggcuac gagcaagccg agaucgaaau gaaacuuccu     3540

aaguaugcuc uguucgaguu ggaaaacggg agaaagagga uggucgccag caauaaagag     3600

gcacagaagg cuaacuccuu ucuccugcca gaacaucuag ugacucugcu uuaccacgcc     3660

aagcaguacg augagauuuc acauaaagag agcuucgacu auguuaauga acaccacaag     3720

gaguuuagug aaguguucgc acggguccuc gaguuugccg gaaaauacac ccuggcugag     3780

aagaacaucg aaaaauugga gaagauuuau aaagaaaauc agacggauga ccuggcgaag     3840

uuagccucuu ccuucgugaa ccucaugcaa uuuaaugcaa ugggcgcucc cgccgauuuc     3900

aaguuuuucg acguuacaau cccucgaaaa agauacacca gccugacuga gauauggcag     3960

ucgacaauua uccaucaguc aaucaccggu cuuuaugaaa cucgcauuag gauggggaag     4020

uga                                                                   4023


<210>  2987
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2987
auggagcagg aauacuaucu gggccucgac auggggaccg gaagcguggg uugggccguc       60

acagauuccg aguaccacgu ucuuagaaag cauggcaaag cucugugggg agugagguug      120

uucgaaucug caucaacugc cgaggaacgg cgcauguuuc gaacgagucg uagaagguua      180

gaccggcgca acuggagaau cgagauucug caagagauau ucgcggaaga gaucagcaag      240

aaagaucccg gguuuuuccu aaggaugaag gaauccaaau auuacccuga ggacaagcgg      300

gauauuaaug gcaacugccc agagcucccg uaugcucugu uuguagacga ugacuucacc      360

gauaaggacu accacaaaaa guuucccaca aucuaucauc uucgcaaaau gcugaugaau      420

acugaagaga ccccugauau ucgauuggug uaccucgcca uccaccauau gaugaagcac      480

agaggucacu uccugcuguc uggggacaua aacgaaauca aagaguuugg aacaaccuuc      540

ucgaagcucu uagaaaauau uaagaacgag gagcuggauu ggaaucuuga auugggcaaa      600

gaggaauacg cagucgugga gucaauccug aaggacaaca ugcuaaauag gagcacuaaa      660

aagacacggc ucauuaaagc ccugaaggcu aaaaguaucu gugaaaaggc aguucuuaac      720

cuguuggccg gcgggaccgu gaagcucucc gacauauuug gacuggagga guuaaaugaa      780

acggagcguc caaaaauuuc uuucgcugau aacgguuaug acgauuacau cggcgaaguc      840

gagaaugaac ugggggagca guuuuauauu aucgagacug ccaaggcggu guacgacugg      900

gcaguacucg uugaaauccu uggaaaauau accagcauuu ccgaggccaa gguggcuaca      960

uacgaaaaac auaagucaga ucugcaguuc uugaagaaaa uaguccgcaa guaucuaacu     1020

aaagaggagu acaaggacau cuucgugagu accagcgaua aacugaagaa cuacucugcc     1080

uauauuggca ugacaaagau caaugguaaa aagguggacc uccaguccaa aagaugcuca     1140

aaggaagagu uuuacgauuu cauuaaaaag aacguccuga agaaacuuga agggcaaccc     1200

gaguaugaau accugaagga ggaguuggaa agggagaccu uucucccuaa acagguuaac     1260

cgggacaaug gagugauacc auaucagauc caccuguacg aauuaaagaa aauccugggc     1320

aaccuacgag auaagauuga ccucaucaaa gagaaugaag acaagcuggu acagcuuuuc     1380

gaguuucgca uucccuauua cgugggcccu uugaacaaga uagaugacgg gaaagaggga     1440

aaguucacgu gggcagucag aaaaagcaau gaaaagaucu acccauggaa cuuugagaau     1500

gugguugaua uugaagcuag ugccgagaaa uucaucaggc ggaugacuaa caaguguaca     1560

uaucugaugg gugaagacgu gcucccgaag gauucucugc uuuacuccaa auauaugguc     1620

cugaaugagu ugaacaaugu gaagcucgac ggcgagaaac ugagcguaga auuaaagcaa     1680

cgccuguaca ccgauguguu uugcaaauau agaaagguua cugucaagaa aaucaagaac     1740

uaccuaaaau gugaggggau uauaucggga aauguggaaa ucacaggcau ugacggugau     1800

uucaaggcgu cucucaccgc cuaucaugac uuuaaagaga uccugacggg gaccgagcuu     1860

gcaaagaagg auaaagaaaa cauuaucaca aauauagucu uguucggcga cgauaagaaa     1920

cugcucaaga aaaggcugaa ccguuuauac ccccagauca cuccuaacca gcugaagaaa     1980

auuugcgcuc uuuccuacac cggauggggc cgguuuucaa agaaguucuu ggaggaaauc     2040

acagccccag accccgagac uggggaagug uggaauauua ucaccgcucu cugggagagu     2100

aacaauaacc ugaugcagcu acugagcaau gaguaucgau ucauggaaga gguugaaaca     2160

uacaacaugg gaaaacaaac caagacucuc uccuaugaga cgguggaaaa uauguacgua     2220

ucuccuagcg ucaaaagaca gauauggcag acacugaaga uugugaaaga gcuugagaag     2280

guuaugaagg aaucaccaaa aaggguguuu aucgagaugg cccgcgaaaa gcaagagagu     2340

aaacggaccg aauccagaaa gaaacaguug auugaccugu auaaggcaug uaagaacgag     2400

gagaaagauu gggucaagga auuaggugac caggaggaac agaaacucag gagcgauaag     2460

cuguaccuuu auuacacuca aaaaggccgc ugcauguacu cuggggaggu gaucgagcug     2520

aaggacuugu gggauaauac caaguaugac auagaucaca ucuaccccca gucaaaaaca     2580

auggacgaua gucucaacaa ucgaguacug gugaagaaaa aguauaacgc cauuaaaagc     2640

gacaaguacc cgcuaaauga aaacauccgg cacgagcgua agggauucug gaaaucccug     2700

cucgauggcg guuuuauuuc uaaggaaaaa uaugagagac ugaucaggaa uaccgaacuu     2760

ucgccugagg aguuggcugg guucauagaa cgccagauug uugagacucg gcagagcaca     2820

aaggcggucg cagaaauccu gaaacaagug uuucccgagu ccgaaaucgu guacgucaag     2880

gccggcacgg uuucaagauu caggaaagac uuugaguuac ucaaggugcg agaggugaac     2940

gaucugcauc acgcuaagga cgccuaucuu aacauuguag ucggaaauag uuacuacgug     3000

aaauucacca agaacgcauc uugguuuauc aaagaaaauc caggccgcac uuauaaccug     3060

aagaaaaugu ucacaagcgg guggaauauu gagcggaacg gagaaguugc cugggaggug     3120

gguaagaagg gcaccauagu cacagugaaa cagaucauga auaagaacaa uauucuaguu     3180

acuagacagg ugcaugaagc uaaaggggga cucuuugacc agcaaaucau gaagaaaggc     3240

aagggucaga uugccaucaa ggagaccgau gagaggcugg cauccauaga aaaauacggg     3300

ggcuauaaca aggcggcugg agccuacuuc auguugguag agucaaaaga caagaaaggc     3360

aagacgaucc guaccauuga auuuaucccu cuguauuuaa agaauaaaau ugagagcgau     3420

gagucuaucg cacucaacuu ccuggaaaag gggcgcggac uuaaagagcc caagauacug     3480

uugaaaaaga uuaaaaucga cacacucuuc gaugucgacg gcuuuaagau guggcugucc     3540

ggucggacug gggauagacu acuguucaag ugugccaauc agcuuauccu cgacgagaaa     3600

auuauaguga ccaugaagaa aaucgugaag uuuauucaga ggcgacaaga aaaccgggaa     3660

cugaaauuga gugauaagga cggaaucgau aaugaggucc ugauggagau uuacaacaca     3720

uucguugaca aguuagaaaa cacuguguau cgcaucagac ucagcgagca ggcuaaaacc     3780

cugauagaca ggcagaagga auuugagcgg cuuucucugg aagauaaauc cucaacguug     3840

uucgagaucc uccacauuuu ucaaugccag aguagcgccg caaaucugaa gaugaucggc     3900

gguccaggca aagcugggau ucuaguaaug aacaauaaca ucucgaagug uaauaagaua     3960

uccauuauca accagucucc cacaggaauc uucgagaaug aaauugaccu gcuuaaaauc     4020

uga                                                                   4023


<210>  2988
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2988
augagauucc agaacaagaa aaccggcgac uacuuugugg ggcuggauau cggaacaaau       60

agcgucgguu gggccguuac uaacgagaag uaugaaauuc ucagggcuaa aaaucaccgg      120

auguggggcu cccgccuuuu cgaggaaggg aagacggcag aggaacgacg ugugcauaga      180

gccggaaggc ggcgccugaa cagaaggaau gaccgguuga ugauauuaga ggaucuguuu      240

gcggaggaaa ucucuaaagu agacaaggcu uucuaccaac gccuacacga gucacaguau      300

cgacaggaac auaagaccgu gaguggcccc uacuuucucu ucaacgauaa agaguuuaca      360

gacgaggauu aucacauuga cuacccuacu aucuaucauc ugagagucgc ccuuaugaag      420

gcaccagcca aagaugugag gcuguuguuc cucgcuauuc accacaucau aaaguaccgg      480

gguaauuuuc ugcaugaagg ggagaccuuc aaaacaaccg gaggccugac ugacauccuc      540

cagcguguuu uagcaugcaa cgaauuuagc ggccugaagc aacacauuga ugacacagcc      600

cuugugaagu ugcugaccaa uaaagggauc acgcgcuccg auauugcuaa gcagaucucu      660

gagcuagcca cugacaaacu cuacagaaag caggcgaaag cauucgccuc gcugguccuu      720

ggacugaagg cuaacuugga cgaucucuuu aaugaaccgg aguauaaaga gcuggacuca      780

gaaauaagga ccguggaguu cagcgguaag gaauacuggg aggaacggga uaaguaugag      840

cccuacuuag gcgagagucu guucgcccuc gacgggugua aaacacuuua ugauacucug      900

uugcuaucca acauuaagcc ugacggaaaa uaccugucug aagcaaaggu agagaccuuu      960

gaaaaacauc acgagcaccu cgaaagccug aaggaucuuc ugaaguccaa uaaagcucua     1020

uauaaccgag uucuccgcga cgaugagaag aaucugaaca acuacaucac auacauuggc     1080

aaaucagagu ucaauaagag augcaguagg gacgaauuuu auaaauucuu aaagcagacc     1140

aucaaggaua ucagcgacaa cgagaauaaa gccgaaauug ugcaacugau ggagcuagac     1200

ucuuuucucc cacugcuucg gguccgcgag aacgguguga uacccuacca gauccaugaa     1260

gaggaauugg agcagauucu gaaugugucc ucagaaaagu auccuuuccu caacaaagcg     1320

gaugggagcg gauuuacgau cgcccagaag auacugaaac uucugacuuu caggauucca     1380

uacuuugucg gccccuugaa uccugcacac aaggacagag auaacggcuu cgcuuggguu     1440

gugcguaagg ccccagggaa aguauauccg uggaauuuug aggacaaggu ggauaucagu     1500

gcuucugcca cagcauucau cgagaaccuc accaauaaau guacuuaccu gauuggagaa     1560

gacgucuuac ccaaguauuc ccuggccuac agcaaauuuu cgcuacucaa cgagcugaau     1620

aaccuucggu acgaugguaa gccuuugucu uaugaaacaa aacaauccuu caucaaggac     1680

ugcuucgaga aggaacaucg aaaaaugacc gagaagagaa uuagggaguu ucugaaacac     1740

cucggcggga cggauggaaa gggcgcucug accgguuuag acguggauau aaaaucagac     1800

cugaagaguu accgcgauau gguucggauc cuuggggacg gcuucgacga aacaauggcg     1860

gagagaauua ucagguaugu gacuuuguuu ggagaagcaa agaaaguccu ccugagcacc     1920

cuagagaucg aguucggcga ugaccugucc gauacacaga agaaacagau ucucaagcug     1980

caauaccgcg acugggggcg acuuucugcc acuuugcuga aaggaauaaa ggguguggcu     2040

ggcgauaauc cagaauguag caucuuagag ugcauggaac agcagccccu caaccugaug     2100

gagguacuuu caagucauaa caccuaucug gccgcaauug aagaguuuaa uaaggagcgg     2160

acagaaccua ccgacaucgu ugauuacaaa uugguggacg aucucuauau uuccccagcc     2220

gucaagcgua gcguguggca gacucugaga auccuagacg agauaacgaa acugaggggg     2280

ucugucccca agaaaguguu cguugaagug gcucgcacaa accgggccga gaagaagaaa     2340

accgauucaa gaaaggaaag gcuccugcaa cuuuacaaaa cuaucaagga cgagggacga     2400

aauuggaccg aggaaauuaa acagcgcgau gcaggcgacu uuaacaguaa gaaauuguuc     2460

cuguacuaua cacagcaggg ucgguguaug uacagcgggg agaagaucac cuccauuggc     2520

gacuuauuua cuucuaagua ugauaucgac cacauauacc cgagaucggu aacaaaagau     2580

aauagcuggg acaaccucgu ccuggugaag gcggaacuua auagggagaa aaaggauauu     2640

uauccuaucg aaaaagacac gcaagaucgu ugcauuuccc uguggaagca gcuacucgag     2700

cagggacuga ucucagagcg caaguacgaa cgguugguua gaaccacucc ccugacagcu     2760

gacgaguuag ccgauuucgu gaacaggcag cucgucgaaa ccaaucaaag ugugaaagca     2820

guaacaaacc ugcuuaagcg acuguaucca gacacuacca uauguuacgu gaaagccgag     2880

aauguuucug auuuucggca ggaacgcgag uucgucaagu ugagaagccu caacgcucac     2940

caucacgcca aagacgcaua ccugaauauc guggugggca acgucuauca ugagaaguuu     3000

acgaauaacc cuaggaacuu cauuaagaau gcucgguccg acaaaaagcg acccuacuca     3060

cuaaaccaca uguucgauua ucugcuucag guugggaaua aagaaaucug gaacccaaag     3120

accgacaaua accucgugaa aaagaugaug agaaauaacg augugcaggu aacacgcaug     3180

cuggucgagc aaacuggagc cuuguacgac gcgaccauuu auaaggccag cgugacaaaa     3240

ucugaauccu acguuccccu gaagaguagc gaugcuaucu uaucugacgu gaaaaaguau     3300

gguggcuuua cuuccauaaa aauugcauac uacucaaucu ucagguuuua uaagaagaau     3360

agugaggagu ucgaaaccca caucauuagc aucccugucc ucaaagccgg gacgcugaac     3420

ucgaaggagg auuacguggc uuauggagaa gagauuauga auaaacagaa guuugaccgu     3480

guugaacuug uguacaaaaa gcugugcaug aacuccuugg uacggauaaa uggcgucaug     3540

uauuaccucg gugggaaaac aaacaauucu cuguauaucg auagcgccau uucauuggug     3600

cugccagaca acaucgcaaa gaagauuaaa gcgguggaga aguacaucgc cagagucgcu     3660

gagaacggca auuacaaacc gcagcccgau caaguuaguc cugaauuuaa caugauaauc     3720

uauaaggccc uuaccgagaa aaugaagcag ccagcauucg ugggacucaa gacuaauagg     3780

gaaaacgagc uggaaaaaga gacaaccauu acugcuuuca ucgccaugac cuugauugag     3840

caguguaaga uguuaaugga aauccugaau cuccugacac agcagaaaac gaccuacgca     3900

cuuaagccca uaggcguauc cgccucucgc agcacucugg ggaugaacau uacaggauug     3960

uccgaguuua ccaucaucac acaaucagug acuggccuac aggaaaaaga gauucgaaua     4020

aucuga                                                                4026


<210>  2989
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2989
auguggggca gcagacuguu cgaggaagcc uccaccgcuc aggacaggcg ggagcaccgc       60

ucuaaccgac guagacucga aaggcggaag uaccgccuug aucuguugga ggaauuauuu      120

guggaggaga ucaauaaaau ugaccccgag uucuuuauaa gacugaggga gucaaaguuc      180

cauuuugaag aucggagucu uaaaaacaca uucauccucu uuaaugacaa gauggaaggg      240

ggauauaacg auaaagucuu cuuuagcaag uacccuacua uuuaucaucu gcgcuccgca      300

cuuaucaaug guaacaagga ggacguucga gccguguucc uggcgauuca caauaucuug      360

aaaaacagag gccacuuucu ccuggcuggg aaugauauaa acaucucucu gucgcucaau      420

gacacguuaa ccgaucuguu cgaauaccuu aagauucauu ugggaaucga gguagaauuu      480

gagaacaaac ugaaggacau ugagaaaauc gcccuagaua agaaucucuc aaagagcgac      540

aaacaaaaga aaauagugca gcugcuugaa acagauuaua agaaacagcu ggcagacguc      600

uucaaguuga gucucggccu gacugccaac auggagaaua uuuucgguaa agaaaacgag      660

cagggguuag aauuuaucaa cgcugagaau aagauuaaga aaauccaauu cgacuccucu      720

uacgaagaga acaucgcaga guuugaucag uucuauggag aauacuuuga gauuauagac      780

aucugcaaga aaauuuacga ugccaucagc cugucccaga uacucgugga cggcaagggc      840

cuuucagaaa guaaaguucu gagcuaugag aagcacaagg aagauuugaa acuacugaag      900

aaagugcuca agaauauuga caaggcuuac gauaacaaag gggaggacag gcuggucuau      960

aaggugcuua aagaggauaa ggacaaagga accaauuacg uaaacuauuc uaagcugucc     1020

aauaacggua cagccaccac uaaagaagag uucuacaagu uuuugucaaa gaaucucgau     1080

cugaaaaagu ucggcaaauu aaacaaggaa gacaucgaga cacuggacua uaucaauagc     1140

gagauucuac ucgggcgguu ucugccacuu cagaaagaaa agaucaacgg aguuauuccg     1200

uaccaauugc agaugaaaga gcugacccag auacucaaua acgcgaguaa guaccugccc     1260

uuccuuaaua agaucgaaga uggcaugacg gugaaagaca agauuaucuc ucugaucaaa     1320

uuucguauuc cuuauuacgu cgguccauug aacgaguaua accgcauaga aaaugggaac     1380

ggauucgcau ggaucgagaa gaaaaagaau gagaagauuu uucccuggaa cuucaaugau     1440

gugguggaca ucgaaaaauc cgccgagaag uucauuaaca aucucacuaa caaauguacc     1500

uacaucaagg aagagacagu ccugccuaaa aauagcauaa ucuauucgaa guuuaugguu     1560

uuaaacgaac ugaauaaccu acaguacggc aauaagaaac ucgauauuga gcugaagaug     1620

aaaauguaua cugaccuuuu cuuggagaag aaagauaaca gaaaggugac cgaaaagagg     1680

auccuggagu acuuucggau uaauggcauc aacgacggga aagaaaagcu cacaggacug     1740

aacaaugaga uacacgcuga uaugaaaucu uaccgagaca ugugcgaaau uaugggugag     1800

aacuucaaua ucaacguagc cgagaauauu aucaacugga uaaccguguu ucauggcgaa     1860

aagaaaaucu uaauuaauaa gaucaaaaac gucuauggga agauguucaa ugaugagcaa     1920

auuuccaagc ugucaaaacu uaacuacagu gacuggggac gcuugagcaa gaaacuccug     1980

acggauauca aguguuauau agacgcagac ggcauuaauu uuccaguguc cauccuagaa     2040

gcuauggagg uuucuaacaa aaaucugaug gagcuccuga gcaacaagua cgaguucauc     2100

ucaugcauug augacaucaa uaacaaguau uuuggugaua gagacauuag ugccuccgaa     2160

cuuauagaug aauuguacgu gagccccgcg gucaaaaggg ggaucuggca gucugugcgg     2220

auucuggagg aaaucguauc aauuaucggc cacaacccua agaaaauauu caucgagaug     2280

acucgccaua augaggaaaa gguucagaaa aguagcagaa ggcacaacuu agaggaacuc     2340

uauaaggcca uucguaagga cccagaguac guggaacuga ucaaagaguu ugauuccaca     2400

accgacacug auucuuucaa gauuaaaaag cuauaccugu auuuuaugca auugggaaaa     2460

ugugcauacu cgaaggagaa gaucgacuuc gauaaacucu ucacagacag cuaugauaua     2520

gaccacauuu acccgcgguc caagaccaaa gacgauucaa uccauaauaa ccugguccua     2580

gugaaggcua aauauaaucg agaaaagacg gacgucuacc ccaucgauau uaacauacag     2640

aagaauauga acaguuuuug gaauuuccug uauaccaaaa aguuuaucac agaggaaaaa     2700

uacaagagac ucauuaggac uucugaccug accgaugagg aacuugccaa auucaucagc     2760

cgccaguugg uggagacauc ccagucaacu aaggcuaccg ccgagguucu gaaacaaauu     2820

uacggcgaca gcgauaucug cuaugugaag gcagaaaacg uaucugacuu ucgguacggg     2880

uccaguagac agaaggauaa aaaugacaua cagaaguuau ucgucaaaug uagggaggug     2940

aaccacuuuc aucacgccaa ggaugcuuau cucaauaucg uugugggaaa cgucuacgac     3000

gugaaauuca caaagaaucc uguaaacuuu auuaagaaaa auaagauggg uaacaaaagc     3060

aacuauucuc ugaaucauau guucugcuuu gacguggaac gcaacggcua caaggcgugg     3120

gauuccucag agaccaucaa aacuguuggg cgaaugauga agaguaauga cguccggauu     3180

acgaagcacg ugcacccaac acguggagag cuuuaugaug caaccaucua caaagccaag     3240

auagcuaaaa cuggcagcua caugccccug aagucgucca aaaacacccc ucuaucugac     3300

gugacaaagu auggugggua caccaagauu aaaaucgccu auuacagcgu cuauaaguac     3360

aaauucucag auaagaaucu caucaaaacu gaaauuagac ugaucccaau acccauuuau     3420

uugaguaaga acgacgauga caucaaagag uacguuaaga auuaccugaa cgauagggua     3480

aauuccaagu uaauugacuu cacaaaaauc uaugauaagc ucuacgugaa cucucugaua     3540

aaaauuaaug gcuaucuuua cuaucuggga ggcaagacga acgaccgcgu guggaucgau     3600

aaugcaguca gcaucguguu ggacgacaaa auugaauacc ucaucaagcg gauuuccaac     3660

gauauaaagu auggguacga gaucaauguu ucagacgaug aacugaugga cauuuacgag     3720

agccuagccg agaaagcuaa caagaaaauc uuuuauaaca gacugaguga uuucgaaaag     3780

aucuuuucua auauggacgu gaaaaagaag uucgucaaac uugagaagaa cgauaaaugu     3840

agggugcucc ugcaguugcu gaauguuuua accgacuccc aaacuacaua caagcucgaa     3900

auggugggac ugaaacauag ccgaucgaac auugguauga agauaucaaa ucuugaggaa     3960

uuuaagguaa ucaacucuag uauuaccggc cuguauucca augagaucga uauuuugaac     4020

aucuga                                                                4026


<210>  2990
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2990
auggagaagg acuacuaucu gggccucgau auggggagcg gauccgccgg uugggcugug       60

accgacgauc aguaccacgu ccuuagagca cauggcaaag ccaugugggg gacaaggcug      120

uucgaaucug cgaacacugc ugaggaacgg cgcaucaauc gaacggcccg uagaagguug      180

aagcggcgca accaaagaau ugagugguua caggaaauau uugcagagga gaucaauacc      240

aaagacucag gauucuuuag gcggaugaag gaaagucgcu auugggccga ggauaaacga      300

gacgaaaacg gcagcugccc cgagaugccu uacucccugu ucauugauga ggacuuuaca      360

gauaaggaau aucacgagaa guucccaacu aucuaccauc uaagaaaaua ucucaugacc      420

acagaagaga ccccggguau uaggcugguu uaccuugcuu uucaccauau gaugaagcac      480

cgggggcacu uccuguugaa uggaaacauc gaagagauac guaaauuucg caagacuuuc      540

gaccaguuuc ucgagguggu aagaaaugaa gagaugggcu ucgcaggcga aaucgaggaa      600

gagacauaca aaaagauuga gaugacccug aaggauguga accugacgag gucugccaaa      660

aagacuaccc ucaucaaauc guuaaagguc acacgggugg aagagaaaga acugcuugcu      720

uugauuucag gguguacugu uaagcugagc aauaucuuua acgacaaaag ucuagaugag      780

accgaacgac ccaagauauc cuucgccgac gcgggauucg acgauuauga ggcagagauu      840

cagucuaauc ucaacgaaaa guacuauauc auugagagcg ccaaagcugu guacgacugg      900

gccgcacugg cugauauccu uggugaauau uccugcaucu cagaggccaa ggucgcgcug      960

uacgagaaac aucacaagga cuugaaacgc cucaaggauc uggugagaaa uaacauuagu     1020

agcgaaguau auaaggagau auuuguugac gugucuggca aauacaagaa uuacugugcc     1080

uauaucggga caaccaacau uaacggaaag aagaaagauu ucgaaggcaa gaagugcacg     1140

aaagaggacu uuuacgcaua uuuaaagaaa uccgucaucg aauguauaaa ugauucagag     1200

aagacugacu ggcugaaacu cgagcuugaa aaggguaacu uccugccuaa gcaauugaca     1260

ggggagaaua gcgugauucc auaccaggug caucuauaug aacugaacgc uaucaucacc     1320

aaucuccagg agaggauucc ccugcuuaaa cgggauggag aaaagauccg ccagauuuuu     1380

acuuucagag ucccuuacua uguuggccca cugaacggca uaaguaucga cgggaaaaag     1440

acaaauuggg ugaaaaggaa ggagggagua auuuaccccu ggaacuuuuc ugacaucgug     1500

gaugaggaau ccagcgccga gucguucauc cgucggauga ccaauaaaug cacguacuug     1560

auucacgaag acguccuccc uaaguauucu cuguuauacu cccgauuuga ggugcugaac     1620

gaacuaaaua acguuagacu caauggugag cugcuuucag uggaguugaa gcagaaaaua     1680

uaugaagaug ucuuccagag guaccgcaag gugacccaga aacggcugaa gagauaucuc     1740

cugacagagg gcaucauuga cgaaaacacu guaaucaccg ggcuugaugg agacuuuaaa     1800

ggcagucuga cagcuuacca cgauuucaag gagaaguuga cuggucucaa ucugaccgag     1860

agggaaaaag aggaaauuau cuuaaacaua acacuguuug gggacgcccg cagccuacuc     1920

cgaaagcgga ucggcaaaau uuguucccag cugucugaua agcaaaaaga gaaccuuagc     1980

aaguugucau aucagggaug gggccgucug aguaagaaau uccucaaugg gaucgcaauu     2040

uccguucagg gagaagagga guggaccauc auaaaggccc ugugggaaac ucaagacaac     2100

uuaagccagc ugaugggugc uaaauacacg uucagaaaug cgguggagaa ggaaaacggc     2160

gagcuugaaa auagggagau uucuuacgag acagucaaag auuugucaau caguccaagc     2220

gugaagcgcc aggucuggca gucccuccug auugugaagg aaaucugcaa aauacaaaag     2280

aaaccgcccc ggaagguuuu uguggaggua gcaaaagaaa agaccaacuc ugggagaacu     2340

gagucgagga aaaagcgacu acaggacuuc uauaaguacu guagcaaaua ugaaaaggag     2400

cuguacgaug aguccaucca gaauaaacuc gaaaagugcu cagagaguga ccugcgcucu     2460

gacaaauuau uuuuguauua caugcagaug ggacggugua uguauaccgg ccaaccuauu     2520

gauuucgacg aacuguggaa caaucagaag uacgauaucg accauauuua cccacagagc     2580

aagacaaugg augacuccau cgauaacaga gucuuaguga aaaggauauc aaaucaggag     2640

aagaccgacg uuuaucccau ucgugccgau auccgcgaga aacggagagg uuuuuggaag     2700

gaacucaaaa acguggggcu gaucagcgag gaaaaguaca agaggcuuac ucgagcuaca     2760

gaguucgaac cugccgagcu ggcaggcuuu auuucucggc aauuggucga gaaucgccag     2820

acgaccaaag ugguagccuc cauccucaag caggcuaugc cagacacuga aauuguguau     2880

guuaaagcca gaacagucag ugaguucagg cacaaguuua acuucauaaa agugcgggaa     2940

augaaugauu uucaucacgc aaaggacgcg uaccugaacg uggucguugg aaacaccuau     3000

cuagugaagu ucacaaaaag ccccgugaau uuuaucgaga accauucuaa ucgauccuac     3060

aaccugaaga aaauguucac ugauggcaga guagaacgca auggggagau ugcuuggauc     3120

ucaggagaga acgguaccau uggcacgguc aggaauauca ugaagaaaaa caauauacuc     3180

gugacccguc gggcccacga agcaacaggg ggauucuuug accagcaacu gaugaagaag     3240

ggcaaagguc agguuccggu gaaggggagu gacgagagac uuuuggauau cgaaaaauau     3300

ggcggauaca acaaggagac uggcgccuac uucguccugg uggaaagcga cgagaaaaag     3360

gggaaaaaga agaggaccau ugaguauguu ccuauccgcc uuaaaaagca gcucgaaaau     3420

aaccgaaaag cucuggagaa uuaccuuugc gaacaaagag gacugacaaa ccccguggua     3480

cuaauugaua agaucaaaau ggacucgcuc uuucgggucg auggcuucuu uauguggcug     3540

uccgguagga cugggaauca guugcuguuc aagugcgcca accaguuagu gcucucuacc     3600

gaggacgagg caacgcugaa gaaaauagug aaguuuguca accgccguaa agaaaauaag     3660

gagauuaaaa ucuaugaaag cgauaaacuu aacgaggaaa ugcugaauag auuguacaac     3720

acauuccucu auaagcugca aaauucaauc uacggaaacc ggcuaggcga gcaagcuaaa     3780

acccugaguu ccaagaaaga gaauuuugac aaacuucaga uugaagagaa gugucucguu     3840

cuguaugaaa uauugcacau guuccagugc cagucugcca cuagcgaucu gcaauuaauc     3900

gaggguccag gcaaggcggg gacacucagg cugaacucca auauuaccaa guacaaaaac     3960

aucaauauua ucaaccaguc accuacugga auauacgaac aggugaucga ccuucagaag     4020

auuuga                                                                4026


<210>  2991
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2991
augggcaagg gguacuaugu gggacuggac auggguaccg gcagcgucgg gugggccguu       60

acagaugagu ccuaccagau ccucagaagg cacggaaaag cuaugugggg cgugcggcuu      120

uucgaaucug caaagacugc cgaggaacgc cgaauguuuc guacggguag aaggcggcug      180

gaccgcagag gguggaggau ugagauauug caagaaaucu ucgcggagga gauuucacgg      240

guagaucccg gauuuuucuu acgcaugaaa gaaaguaagu auuacccuga ggacaaacga      300

gauauccagg gcaacugccc agaacugccg uauacccuau uuguggacaa gacauucacu      360

gauaaggacu uucauaaaaa guaccccacc auuuaucacc ucagaaaaau gcugauggag      420

acagaggaua ccccugacau gaggcuuguc uaccuggcuu ugcaucacau gaugaagcau      480

cggggccacu uccuccugag cggggauauc ucccagguga cugaauuuaa aaauacauuc      540

aaccaguuua uagagaauau ccguaacgaa gagauggacu ucgaaauuga gcuggaugag      600

ucugccguuc aaaugaucga agagacccuc aaggacaaga auuuaacgcg cucggcuaaa      660

aagucaaaac uggugaaggg acuuaacgcc aaaaauguca gagacaaggc auuuuugacu      720

cugcuaagcg guggcaccgu gaaacucagu gaucuguucg ggauggagga gcuuaacgaa      780

ggagagaggc caaagauuuc cuucgcagac aauggcuacg aggaauaugc cgcuguaguu      840

gagauggaac ugggugaguu guacuauauc guggaaucug ccaaggcggu cuacgauugg      900

gcaauacuca gcgacauucu agggggaucc acaucaguga gugaugccaa agugcgggcu      960

uaugagaagc acaaagccga cuuaaaguac cugaaagcag ucguuaagga guauuuuccc     1020

aaggaagugu acaacgcugu auucguggag agcucugaua aacucaauaa cuacccugcc     1080

uauaucggca ugacuaagaa aaauggcaag aaagucuccc uugaagggaa gcgauguuca     1140

cgcgaggagu uuauggacuu ccugaagaaa aacauugugg uuaaguugcc agaugaagag     1200

gcgaaaaugu accuacagag cgaacuggag aaggacaguu uucuccccaa acaggugaau     1260

aaggauaacg gagucauccc uuaucaggug cauaaguacg aacugaaaaa gauccuugac     1320

aaccugggug acaaaauucc auucuugaag gagaaugccg agaaaauaga aaagcucuuu     1380

ucuuucagaa ucccguauua cguaggcccc cugaggaccg ggaacggaga aaauuccaaa     1440

uuugcauggg cugaacgcaa gagcacagag aagauuuauc cuuggaacuu cgaaaauguu     1500

aucgaugugg agcaaucggc cgagaacuuu auacggagaa ugaccaauaa augcacguac     1560

uuaauuggcg aagacguccu gccaaaggau ucucuacucu acuccaaauu cauggugcug     1620

aacgagcuua auaacuugag gcuggacggu cagaagaucu cagucgaacu caaacagaag     1680

aucuaucgug auguguuuug ucggagucga aagguuacuc agaaaaagcu gaaaagcuac     1740

cuuauuagag aggggaucgc uggcaagaau guggaacuga caggaauuga cggcgauuuc     1800

aaaggguccu ugaccgccua ucacgacuuc aaggagaagc ucauagaugu agagcugacu     1860

caaaaagaaa aggaggaaau cauuuuaaac aucguccugu uuggagacga uaaaaagcua     1920

cucaaacaga ggcugaagaa gaaauucccc cagcuuacag agaagcaaau caauaccgug     1980

acggcauugu cuuacaaagg uuggggccgc cugagcaaga aacuccugga ggaaguuacc     2040

gugccugccc cagagacagg ggaagucugg aacauuauaa cugcuuuaug ggagaccaau     2100

gacaacuuua ugcagcugcu uucaaaggaa uaucaguuca gugaucggau cgaggaguuu     2160

aacgaagaga aagcggaccc cgaagugucc uaccagacaa uugaggacuu guauguaagc     2220

ccggcaguga agagacaaau cuggcagacu cuccagauua uccaggaaau agagaagguu     2280

augggagaga aaccuaagag ggucuucgug gaaauggccc gcgagaaaca agaaaauaag     2340

cgaaccgagu cucggaaaaa gacacuguca gaucuauaca ccaagugcaa aaaggaggaa     2400

cccgagugga ucgaagcucu guguacgagu cucgagaaac augaggaaca ccagcugcgu     2460

agcgacaaac uuuacuugua uuacacucag aaaggcagau gcauguauuc cggugagcca     2520

auugaucugg aagacuuaug ggauaacaca aaguacgaca ucgaucacau uuauccucag     2580

ucuaagacca uggacgauuc gcucaaaaau agggugcugg ucaagaaaga guacaacgcc     2640

aagaaaagcg acacuuaucc caucgcagcc gauauacaaa agaagaugau gccauuuugg     2700

aaaucccuuc ugaccggggg cuucauuccc aaggaaaaau acgaccgcuu gguucggaau     2760

aacccucucg augcuaauga gcuggccgga uuuaucgaaa gacagauugu ggagacaagg     2820

cagucaacca aggcaguggc ggagauccug aaaaagauac ugccagacac ugaaaucgua     2880

uacgucaagg cuaaaacagu gaguaaguuc cgacaggacu uugauuucau uaaaguucgc     2940

gacaugaacg aucuccauca cgccaaggac gcauaucuga auaucguggu cggcaacgug     3000

uacuuuguua aauucacgaa gaaugccgcu ugguucguga aagagaaccc ggggcggacc     3060

uauaaucuug aaaagauguu uauuugggau guagccagag acggagagau cgcauggaag     3120

gcugguaaau cuggcagcau aguccaagug agggccauga uggaaaagaa ccauauuuug     3180

gugacucguc gcuccuacga ggucaaaggg ggacuguucg aucagcagau cuuaaagaaa     3240

ggcaaggguc aaguucccgu gaaggaguca gacgaacggc ucagagauau cgggaaauau     3300

ggcggauaca auaaggcggc cggcguauau uuuaugcugg ugagcucuaa agacaagaaa     3360

gggaaggagc agaggacaau ugaguucguc ccuaucuacc uuaaggaucg aguggaaaaa     3420

gacauggaau ccgcucggaa cuaccugaug gaggagcgcg gacuaagaga accaaagauu     3480

cucauaaaga agaucaaaau ugacacccug uuuaagguug auggcuucua uaugugguug     3540

agugguagga caagcaacca gcugaucuuu aagggggcaa aucaguuaau ucucucuaaa     3600

gaggacauga agacucugaa aaaggugguc aaauacaucc aacggcagaa ggaagauaaa     3660

aacgugaaga uauccaugcg agacggaauc accgaggagg aacuucugaa guuguaugag     3720

acguuccucg auaaaauuag acacucaauc uacggcgccc gccugaguag ccaggaaaag     3780

acccuaguua augguaaaga gaaguuuauu gaccugucgg uggaagcuaa augucagguc     3840

cuuuaugaga uccuccaucu guuccaaugc caguccggcg ccgcaaacuu gaagaugaua     3900

gggggacccg cgucugccgg cauucuggua augaauaaca auaucagcaa guguaaaaac     3960

aucucaauug ugaaucagag uccuacaggg auauacgaga aggaaaucga uuuacucagg     4020

guuuga                                                                4026


<210>  2992
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2992
augacccaga aggugauccc cuacaacauu ggccuggaca uagggacaaa uagcgucgga       60

ugggccguua cugauaacga gaauaaacuc cuucacauca agggucauaa cggcauuggg      120

gugagacugu ucaaagaagg acaauccgcu gcagagaggu gcggcuuucg gacgacccgc      180

cgacguuugu cuagaaggaa guggcgguua cgccugcuaa augaaaucuu cgccgacaaa      240

auugcgaagg uagauccuuc auuuuucgcu agacucaagc agaguaacgu gagcccaaaa      300

gacccgaaua agacaauguu ugguaacauc cuguucgaua augggaaccu uaaugacaaa      360

uccuuucacg augaguauaa gacuauauac caucugaggc agucuaucau ugaccacccc      420

gagaaaaagu ucgauauccg guugauuuau cucgccaugc aucacaucau aaaguaccgc      480

ggacacuuuc ugaaccaggc aaauguccaa gacuucaaag gcggcaagau ugaucuggaa      540

gagucguuua aagaccucaa cucaaucuuc gaaaaucagg ggcgagauuu aagacuggug      600

accgaggacg ccaacaagua uguuaaagaa cuuagcgaca acaguaagac aaggaaugau      660

cggcagaaag aguuguccaa ggugcuguac auuccuaucg acaagaacuc ugauaaaaau      720

aacaagaaag ucaccacuga gaucauuaag gcuauacuag gaaugaaagc aaaguuugac      780

gugaucuucg guauugaugc cgaaaaugcu aaggagugga gccucacauu caacuccgac      840

gauuuugacg auaaaaucga aggccuguca gcccagauga ccgacgaggc gggggaaaua      900

cuucugauuu ugaaggagcu cuauuucgca cugaauuuaa gugauauccu gaacaauagc      960

guaacggaca aaaacgccaa gacucucucu gaggcuauga ucaaucguua cgacgaucau     1020

aaagaacaac uuaccaugcu gaagcagguu auugacaacu ccgauaagaa acacgccaag     1080

gcauugaaag cugccuacuc agcguauauc gacggagaga auaacaagaa aauuagcuuu     1140

gaagaucuac gcaagagaau acagaagaau cuggacaaca gugagacauc uaaaaagauc     1200

aacgaucucc uggaacauga cuacuuccuu ccaaaacaga gguccaagga gaauggcgcc     1260

auuccccacc aacugcagca gcaagaguug gauaaaauca ucgaaagcca gaagcacuau     1320

uacccuuuuc ucgcagagcu gaacccaaau gaaaaacggu cgcgacaggc uaaguauaag     1380

uuagacgagc ugguggccuu ccgcgucccc uacuaugugg guccuauggu gaaagcuacu     1440

gaaaacaauc agucuaacaa ugggaaguuu gccuggauga uuaaaaacga uaauauaucc     1500

gacaagcuca aaaaggagga gaucacccca uggaacuucg auaagaaagu cgacagaaca     1560

gaaaccgcaa cgaaguuuau uacuaggaug acaaccacug acacauaccu aaucggagag     1620

ccgguucugc ccaaaaauuc acuuuuguac caaaaguucg ugguacugaa cgaacucaau     1680

aaccugaaaa uuaauaagca gccuaucacc ccagagcaga agcaguaugu guacgagcau     1740

gucaugaaac aaacgaagcg ggugagucuu aaaaagauag ccgaugcucu gguuaucaaa     1800

ggcgacuauc ccuacgcgcc ugaaauuacc ggcacaacug aucagaagaa caugaacaau     1860

ggguugagca ccuauaucga ccucgcaaag auuuuuggag augagcugga cgauaucgac     1920

cgcaaagaag auuucgagaa gauaauugaa ugguccacaa ucuuugagga ccagcacauu     1980

uucgccuuaa aacugaagga gaucaaaugg cuaacugaag aucagagaaa gcaagugguc     2040

aaacucaggu accaggguug gggcaagcug ucuaagaaac uuuuggcuga ccugguggau     2100

gacaacgggc agcguauaau cgaccuccug ugggauagca agcaggacuu caugcaaauu     2160

guaaaucaga acucauuuaa acaggccauc gcagagcaua auggagccca cuuagaagag     2220

aaggaucuga aagacauuau caacaaucuu uauaccaguc cacaaaacaa gaaggcuaua     2280

cggcagguua uuuugguggu cgaugacauc auccgagccg ugggcuacga acccgucaau     2340

auuaugaugg aguucgcaag agaggauucc aaagaccaua ggcucacaaa cagccgcucu     2400

cggcagcugg aaaaggugua caaagauauc accaauucau gguuugacga ugacaacguu     2460

aaggacgagc uaaaagauaa gauuaaggac aaagcgaagu ucacugauag acuguaucuc     2520

uacuuuacgc agggugggaa agaccuguau acaggcgaac cgcuugauau agacaauuug     2580

aguaacuacc aaaucgauca cauucugccu cagagcuuca ucuuagacaa uucccucgau     2640

aacagggugc ugaccucuaa gauuaacaau cagaucaaaa aggacaaccu ucccggagag     2700

uuuuucggcu cgaagaugca caauuuuugg aaacaucuga aggaauugca ccucauaagc     2760

aacaaaaagu auuacaaucu gacucuaacc ccaaacucca ucucaaaauu caauaaccag     2820

gggcgcuuua uuaaucgaca acugguagag acacggcagg ucaucaagcu cgcugccgag     2880

auucugcaua accguuauag ugaagagaau ggaaccaaca ucgugacugu uaaggcaaau     2940

cuuacacacc acaugagaga aaaauucaac uuuuacaaga auaggaacgu gaaugauuac     3000

caucacgccu ucgacgcuua uuuggccgca uucgucggua acuggcuguu acagcaguac     3060

ccuaaacuca agcccuauuu uguguacggc gauuucgcua aaacggagau agacaaccug     3120

aagucuuuua aucuuauuua uaaauucgag caagcccauc agcugaacga agaggacggg     3180

aagaucgcga aggauaccga cgcccuacuc ggauacauga aaaauguaua ucacuuuaag     3240

aaaaugcugg ugacuaagga auuggagaca aaccauggca aucuguucaa acagaccuua     3300

uacccaagcc acgguaagau gaagaaccuc aucaauccca aagaaaaccg cuccucagau     3360

auuuacgggg gcuauagcuc ugagacagga gcuuacguuu cccugauaaa ggucuuuaaa     3420

aagaauaaaa ucgagaacaa gauuaucaag auuccucuuc uguauaucga caaauugaau     3480

cggcaagcaa acgccaagca guucauaaga aauuuuauca uuaaagauga caguaacaag     3540

uucgauaucc ucagcgagaa aauuuuucug aaucagcuag ugaucgacgg cgaugaacug     3600

uucacuauau cuggguccgg agacaggcag aacgcucaac agcuuauucu cucagauaag     3660

agucugaaga ucuugagcaa aaacaucaau gugauuucga agaacgaccu gaucauaauu     3720

uucaaugaaa ucauugauga caucuguaaa uacaugaaga uguaugaggu cgaaaacucc     3780

gacgagacca aagccaaguu ugaggaaaua aaugauaaag accgaauguu aaagauuauc     3840

aacgaucucc ugcuuggucu gcacgcaaau gcgucucgca gcaacuuguc aaguaucggc     3900

cucuccccau cuuucgggcg guuuuucagc ggaaaugccg gcauuaagcu guccgagaac     3960

gcuaaauuua ucuaccaguc accgacgggu cuauucagca gaaagguuaa aauuagugac     4020

cuguga                                                                4026


<210>  2993
<211>  4032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2993
augaccuacu aucugggccu cgacaugggg acaagcuccg ugggaugggc cgucacugau       60

gagaacuaca aucuuaucca cagacauagg aaggaccugu gggguauucg ggaguucgaa      120

gaagcuaaaa cggcaguuga gcgccgaacc cagcguauau cuagaaggcg gcgccaaaga      180

gaaguggcca ggaucggcuu guuaaaggau uauuuucacg acgagauuca gaaaguagau      240

cccaacuucu accagcggcu ggacaauuca aaguauuuuc cugaggauaa agaagaggug      300

gucaagggga agaacggaau cuucgcggac gaaaauuaca cagaucgcga cuauuuugcu      360

gaguacccaa cuauuuucca ucuacgaaga gagcuccuga acaguaaaga accgcacgau      420

gugaggcuuc uguacuuggc ccuccugaau auguauaagc aucggggcca cuuucugaac      480

accacacucg guaccgacgg ggagaaucgu aaauuaagcg auguuuaccg cgacuucgug      540

gucaagacug aagaggugcu gggacuuaca uuuccccagg aaaccgacaa cggcaaaaug      600

caagagauca ugugcuccag agaguucucu aggaagcgga aggcagaauu gcugugugcc      660

cuacucgaga uagauaaaaa gcagaaacag cagguagcuc ugaucgaaug cguucuuggc      720

cuggacaagu cggugaaaca aauguauggg acggcagucc cugaugacuu ugauaaguca      780

gugaaaguga auuucagcga caguuccuuu gaggaaaagg ucucugaggu ugaggaauug      840

cucgaugagg aaaaguacga gcugauuuua uguaugaaag aacuguauga cacugccgug      900

cucaaccaga ucaugggaga uugcaauuac cuuagcgagg cucgaguaca ggaguaugaa      960

aagcacaaag aggaccugaa guugcuaaaa cgcaccguga agaaguacgg uacagccgaa     1020

gaguaccagu uccuguucag auccucagag aaagcgaguu auaacgcaua cguccauagc     1080

guuaauucug gcgaaaagau uaggcggaac auggaugggc gcuccagaga cgaccucuau     1140

acugugcuga aaaagaugcu uaaaaacuac ccacaagagg augccgacug uaaguauauc     1200

cuggaagaga uagaaaagga gcaguuuuug cccaaacagc ucgugucaga uaauggaguc     1260

auuccuaacc aaauccacua caaggagaug aaaaagauuc uggaaaaugc ugagcaguau     1320

uuacauuucc ugagggaaaa agacgagagc ggcuacaccg uggccgaacg uauccuaaag     1380

cucuuugcau uccagauccc auacuauauu ggucccacaa gugagaaguc ugggaaaaac     1440

ggaggcaaug gcuggguagu ucggaaggag caggggcaag ugcugccuug gaacauagaa     1500

gagaaaaucg auaugaaggc uaccuccgaa acguuuauua gccgaauggu cagaaggugc     1560

acuuaccuuu cggacgagau gguguugcca aaagccucuc uggaauauga guccuucugu     1620

guccucaaug agcugaacaa ucuucgcauc cggggaguga agcugccgau aacaguuaaa     1680

caggauauuu acaaggacuu guuugaaaga gguaagaggg ugacccgcaa acagaucgua     1740

aacuaucuca agggcgucgg gauccugcag gagggagaag aggaugaguu aacuggcaug     1800

auggacacau ucaauucaag ucuggcgagc uacgguaaau uuaaguccau ucuaggggag     1860

aaaauccaag aagauucuug caagcgaaug guggaacaga uuauauucug guguaccauc     1920

uauggcgacg ccaagaaauu ucucaaggag cagcuggaag agaaauacgg agauguucuu     1980

acgcgggagc agauuaagcg uaucuugggc uucaaacuga aggacugggg gaaccucagc     2040

aagaaauuuc uggaauuaaa uggaugcgac agaaacaccg gugaggugau gucacugauc     2100

aggaugcuuu gggaaaguga ugacaauuug auggagcucc ugaaggauuc cagcuacaca     2160

uaucgcgcag aacuagagga gcaacaggaa aaacugacua agacccucac agagauuugu     2220

cccgaagacc uggagggcua cuauuucucu gcuccuguca aacggaugau auggcagacu     2280

cuuuugaucc ugaaggaagu ggagaaggua augggggagc cacccaaaag aguguucguu     2340

gaaaugacca gggagcacga ugaacgacgc gagaugaagg acucacgggg caaaaaguuu     2400

caggaguuau acaaaaacau uaaggaugag gaacacaacu ggaaagccga aaucgaggac     2460

gcugaaaagg auggaagaau uaggaguaag aaaauguauc ucuaccugac acaacguggu     2520

cgcugcaugu auaccgggaa uccuaucgac cuugaggagc uguucacuac gaaguacgau     2580

auagaccaua ucuacccacg gcacuuuguc aaagaugaca acuuggacaa uaaccucgug     2640

cugguggaaa aggagaaaaa ugcccauaag agcgaucagu auccccuagg cgacgauauu     2700

uacacaggaa gacuguccug guggaaggaa cuccacaggg agaaacugau caacgacgaa     2760

aaguauaaac gacuuaccgg ccgggcaccg uucucugaug agcagaaggc cggguuuauu     2820

gcucgccaau uggucgagac uucgcaggga accaaaggug uugcgagcau ccugaaggaa     2880

guguuaccug agacaaccac uauaguguac gcaaaggcca gaaauguauc cgacuucagg     2940

aacacaaaug aacuccugaa agcucgguca gucaacgauu uucaucacgc ccaggacgca     3000

uaucuuaaua uugugguugg caacguguac uaugucaagu ucacgaauaa cccccugaau     3060

uuugugcgaa guggggaggg auacaaccua gauggcguuu uccgcaaaaa cgugaucaga     3120

ggucaggaaa augccuggau uguaaagcca gagaaagggg agaagaugac ccucgaaacu     3180

gucaagaggg ugauggcuaa aaacacaccu cuguugaccc gucugucuuu ugagggccgg     3240

ggaggcuuag ccaaucaaac acucuacagc gcaaaggaag cggcugggga gggauaugug     3300

ccccugaaau ccucagacag cagacuucag gaugucacua aguacggcgg uuucaccucu     3360

guuacgaccg ccuauuuuau ccugguggag cacgacauga aaaagaagag gguacgcaca     3420

auagaauccg ugccaaucuu guggaaagag cgaauugaaa aggagagugg gcagcucgaa     3480

aucuacugca gagaggcacu gggccuaguc aaccccgaca uucgggugag gaaaauccug     3540

auacagagcc uuguuaagaa agauggauuc uauaugcauc ucucugguaa gacuggcaau     3600

cgcuucauua ugcguaacgc cguggagcug uguuugaaac aagaauggau caauuacucc     3660

aagaagcugg agaaagaagg gugcgacgag ucaaucacca aggagaaaaa cgaagaguua     3720

uaugaaauuc ucaaggagaa acacaucaau ggaauuuaca gaaagcggcc uaacccaaug     3780

ggcgaaaagc ugcauauaag gcaggagaca uuucgcaugc uuacucugga ggaacagaaa     3840

aaggucuugc ucagucugcu aaaucugacc aaaaucgggc cgguggaggc uaaccuugaa     3900

cucauuggag agagcgccca cacgggcaag augcugaucu cgaaaaauau uacagaaguu     3960

aaggaguucu acuugaucca ccaguccgug accgguauau augagaagca agucaacuua     4020

cugacuguau ga                                                         4032


<210>  2994
<211>  4032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2994
auggccacca agaaagagua caacaucggc cuggacauug ggacaacuag cgugggaugg       60

uccgucguug aaacggauaa ucagaaggug augagaaaag guaacaaggc ucucuggggc      120

guaaggcuuu ucgaggaagc aaccacagcc gagucucggc gcaugcaacg aucaacucgu      180

agaagguaug accggcgcag agaaaggaua aaacuguugc aggaggaguu uagugaagag      240

aucaauaagg uggauaagaa cuucuuucag aaauuaaagg aaagcaaaua cgucgagaau      300

gacaagauua acaaaaagau cgugcugacc aaggaggaaa aacaggagcu aaaggauuau      360

caaaauaaau acaagacaau uuaucaccuc cgggacgaac ugaucaacaa ucccgagaaa      420

aaggauauac gccuuguuua ccuggcgauc caucacauua ucaaauaucg agggaacuuc      480

uuguaccaga augcuaacuu uaauauugac aaccucgaua ucaaggacaa gcugaaugaa      540

cuguucucca uacucucuaa caacauucag gaguuagaga ucccugaaga uuacauuaau      600

aucaucgacu uugaugagcu ggaagccgac cuuuugaaag acaccaagaa cgaugugaaa      660

gcacugcuaa uugacaaucu cacugagcug acaaacaagu cgucagccac cgaguucgga      720

aaacuuaugg ucggcaauaa ggguaacaua aauaagcugu ugaugcucga gacggauaac      780

aaaaucgaga uuagcuuuag ugggacugac uaugaugaca aguacgaaga guaucaggaa      840

gcucugggag agaauaucga aauauuagau auucugaaac aaaucuacga cugcaucuuc      900

cucaagaaaa uuuuuaaggg caacaagaau accuccaucu cuagccuuau ggugaaaucc      960

uucgagcagc auaagaaaga ucugaacuuu uugaaggagc uauucaaaga caauagagaa     1020

cuguauaacg agauuuucag gacaaagaag gaucucuguc uguacgaaaa auauauaacu     1080

aauaagaucg acugcgacga guuucggaaa gagauuaaca aacuucugga aucauuguuc     1140

agugaugaga acaucaagca gaaucuccug gacgaauaca acuuagaggu aaaggaacgu     1200

aucgagaaug gcaccuuucu gccacgcauu acaaccacgg auaacgggaa auacccguau     1260

cagcuaaaua agagcgagcu cauaaagauc auugaaaacc aaggaaaaua cuaucccuuc     1320

cugcuuaaua aguugaacga caauaaaacu uacaagaucg uuaaacugcu cgaguuuagg     1380

auuccuuauu acgugggucc acuggucucu gaaaagaggu ccgagaacuc auggcuugaa     1440

cggaagauca auaacgugaa aauaacaccc uauaauuucg augacguggu cgauaaggag     1500

aaaaccgcag agaaguuuau caaacgaaug auuagccacu guacuuaccu guuggacgaa     1560

uacgcccucg cuaacaauag uauccuguau ucugaguuca agguuaugaa cgaauuaaag     1620

cagauucgcg ugaacgauag aaaacuggag cuaaaguuuc agcaacagau ccucgaagac     1680

uucuuuaaaa agacauccgg caccauaacg gagaggaaau ucaagaauua ccugauuagc     1740

accggggagu auucgaugga aggaggcaac uuucggauca cagguuacuc ugccgauggg     1800

aaguucgcga auaacaugca guccuaugua gacuucuuug gagagaaugg cauuuucgca     1860

gauacugacu acaaccuuga aaaugccgag gagaucauag aauggaucac cauuuuugag     1920

gacaaagaua ucuuggaaaa gaaagugcgc gacaacuauu caaagcugaa ugagaaccag     1980

auuaaaaagg uccucgcuaa aaaguacagu ggcuggggga gccuguccaa gaaauuacug     2040

aagacaaaau acuauaagga uaaagccacu gaacuuuaca agucuaucuu ggaccucaug     2100

uaugagaccg auaagaauuu caugcaaaua auuaacgacg auaaauacaa uuuucaggag     2160

augaucaaga aauucaacaa uaaggaagag aacaaaaagc ugaacuauag caucguggaa     2220

gaacuagcaa caaguccugc uaccaagaga ggaauuuacc aggcccugaa aguuguggag     2280

gaacucgucg acuauaucgg uuacgagcca aagaauauua guauagaaau ggcgaggucc     2340

gaggaagaga aagugcguaa ggauagccgg aaagacuaca ucaacaaucu guauaagggc     2400

ugcaaggauu cuauugaaaa cuacaaaaag cugaaacaug aguuggacuc acacgagauc     2460

acuucacagc gacuguuuuu auauuucauu caagaaggga agugucucua cacgggaaca     2520

ccccugaaua ucgaggauau agaaaaccag agccuuuaug agaucgacca cauuaucccu     2580

agaucccuga uuaaagacga uucuaucgac aauaaggccu ugguacucaa ggagugcaac     2640

caggauaaaa aggcaucgua cguucugcca agcaaauuua gaaauuccaa gcagaaaacc     2700

ugguggaagc aucuaaaaga caacggccug augucagcua agaaguucca caaucucaua     2760

cgcgaaaaau auaacgagga agauauuaau gguuuuauca accggcaacu gguggagacu     2820

agacagauca ccaagcaugu cgccaauauu cuuaacaauu uguacaaaga cacaaacgug     2880

auauaccuga aggcugauuu aagucacaac uauagggaaa aauacgaccu cuucaaguuu     2940

cgcgauauca augacuauca ccaugcccac gaugcauacc uggccgcugu ccuuggggag     3000

uauaaggagc gauucaugaa acggaagauu aacuucgaca uggugaaaga acuuaauucu     3060

aagaucguug agauggacga aggcaaacgu agaaaccuaa gguuuggaua cgugauuaau     3120

agccucgaug agaaccugaa ugacaucgua uugaagauau ccgaaaaccu ggucgauaau     3180

gagaccggcg agguguuauu cgacgcgcau gaauuuaaca agcgcguuga ggauacucuc     3240

uaucggaaug acauccugau uucaagaaaa acagaaauca ggacggggca guucuacaag     3300

gagaccauuu acaaaaaggg aaaagguacu aucccgauaa aguuuggcau gcccacagau     3360

auuuaugggg gauacagcaa caccaaugca cgaaaacuua ugcugaucga auauaacgag     3420

ucuaagauga agaucauugg cuugccuaug gcccucgcug ugcgcaauaa cgacgccgag     3480

aucaacgauu acauaaaaac acagauuaau cugaagaaaa acuccacuuu caagaucaaa     3540

aaguguagua uuccauuuga aaccgagauc auauauaaua accaaaaugu cuucauuaag     3600

gguuacagca ucgggcacaa aaacugcgaa aucucuaaug caauucagcu aaagaucgcc     3660

aaagacaaga ugaaacauug gaaguaugcu cugcaguacc uucucaacga uaagaaacaa     3720

uacauugacu uugccgagaa guaugugaau gagauauacg auuuccugau caacuuggac     3780

cuguuucccu uauucaauaa agaacucgac aagauuaaaa acaagguaaa uuucgaggau     3840

cuggcacuug accagcagaa gaaaaucacg aaggaacugu uuaaaauguu gcacuguaac     3900

uccgugaaug cgaaccucuc agaguucggc cugggagauc ggaugggcag acuaaguggg     3960

uauaacauca ccgacggaau uauaaucagc aagucgguua cagguauuag ggaaucccgu     4020

uacgaguuuu ga                                                         4032


<210>  2995
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2995
augagccaga acaauaacaa gaucuacaau auuggccugg acauagggga ugccuccgug       60

ggaugggcug ucguugacga gcacuauaac cuccuuaaaa gacaugguaa gcacaugugg      120

ggcucuaggc uguucaccca agcaaauaca gccguggaac ggcgcucaag ucgaagcacu      180

cguagaaggu acaacaaacg gcgcgagaga aucagguugu uacgggaaau uauggaggau      240

augguacugg acguggaucc cacguuuuuc auccgccuag cgaaugucuc cuuucucgac      300

caggaagaua agaaagacua ucugaaggag aacuaccauu cuaauuauaa ccuuuucauu      360

gauaaggacu uuaaugauaa aaccuacuau gacaaguacc cuacaaucua ccaucugcga      420

aaacacuugu gcgagucgaa ggaaaaagag gauccaagac ucauauaucu ggcucugcac      480

cacaucguga aguacagggg gaacuuccuc uaugaaggac agaaguuuuc aauggacguu      540

agcaauauug aggacaaaau gaucgaugug uuacggcagu ucaacgagau uaaucuguuu      600

gaauacgucg aggaccguaa gaaaaucgau gaagugcuua acguauugaa ggagccgcug      660

aguaaaaagc auaaagccga aaaggcauuc gcccuauuug acacuaccaa ggauaacaaa      720

gcugcauaua aggagcucug ugccgcucug gccggcaaua aauucaacgu uacaaagaug      780

cuuaaagagg cggaacugca cgacgaggau gaaaaggaca uauccuuuaa guucucugau      840

gcaaccuucg acgaugccuu uguggagaaa caacccuugc ucggugacug cgucgaguuc      900

auugaccugu uacaugauau cuacagcugg guggaacugc agaauauucu cggguccgcu      960

cacacuucag agccuaguau cagcgccgca augauccagc gcuaugaaga ccacaagaac     1020

gaucuuaaac uguugaagga cgugauuaga aaauaccuac caaagaagua cuuugagguc     1080

uucagggaug aaaaaucuaa gaaaaauaac uauuguaauu acauaaacca ucccuccaag     1140

acaccuguug acgaguuuua uaaauacauc aagaagcuga uugaaaagau cgaugaccca     1200

gaugugaaga ccauacucaa uaaaauugag cuggagucau ucaugcuuaa gcagaacagc     1260

cggacgaaug gagcuguacc cuaucaaaug cagcuggacg aauugaacaa aauccucgag     1320

aaucagagug uguacuauuc ugaucugaag gacaacgaag auaaaauccg auccauuuua     1380

acuuuucgca ucccuuacua cuucggccca cugaauauua ccaaggacag acaguuugac     1440

uggauaauca agaaagaggg caaggagaac gaaaggauuc uaccguggaa cgccaaugag     1500

aucgucgaug uggacaaaac agcggaugag uucaucaagc ggaugcgcaa cuuuugcacu     1560

uauuuccccg acgaaccugu uauggccaaa aauagccuca ccgugucgaa guacgaaguc     1620

cugaacgaga uuaauaaacu uagaauaaac gaucacuuga ucaaaaggga caugaaggau     1680

aaaaugcugc auacacucuu uauggaccac aagucuauuu ccgcaaaugc uaugaaaaag     1740

uggcugguga agaaccaaua uuucucaaau accgaugaca ucaaaauuga gggguuccag     1800

aaggaaaacg ccuguaguac gagccuuacu ccauggaucg auuuuacaaa aauauucgga     1860

aagaucaaug aguccaacua cgacuuuauu gagaaaauca uuuaugaugu aaccguuuuc     1920

gaagacaaga agauccugcg ucggcgauug aaaaaggaau acgaccucga ugaggagaaa     1980

auaaagaaaa uucugaaguu aaaauauucu gguuggagca gacugucaaa gaagcuacuc     2040

aguggcauca aaacuaagua caaagacucc acaaggaccc ccgaaacggu gcuggagguc     2100

auggaacgca ccaauaugaa ccuuaugcaa gugauuaaug augagaaguu gggguuuaaa     2160

aagacaaucg acgaugcuaa cagcacuucu gucucaggaa aguucaguua cgccgaagug     2220

caggagcugg caggcagccc ugccauaaaa cgggguaucu ggcaggcucu ccugauuguu     2280

gacgagauca agaaaauuau gaagcaugaa ccagcgcacg uguauaucga guuugcaaga     2340

aacgaagaug agaaagagag gaaggacucc uucguaaauc agauguuaaa gcuguacaaa     2400

gauuaugacu uugaagauga gaccgaaaag gaggccaaca aacaccuuaa gggggaagac     2460

gcuaaaucua agauacgcuc ggagcgauug aagcucuacu auacacagau gggaaaaugc     2520

auguacacug gcaagagccu ggacauugau cggcuagaca ccuaucaagu cgaucauauc     2580

gugccccagu cccugcucaa agacgauuca aucgacaaua agguucuggu gcuuaguucu     2640

gagaaccagc guaaauugga ugaccugguc auuccgagcu ccaucagaaa uaagauguac     2700

ggcuucuggg aaaaguuauu uaacaauaaa auuauaucac cuaagaaauu cuacagccuc     2760

aucaagacag aguuuaacga aaaagaucag gagagguuca uuaaucgcca aaucguggaa     2820

acccggcaga uuacuaagca cguagcccag aucauagaca accauuauga gaauacgaaa     2880

gugguuacag ucagagcaga ucugucucac caguucaggg agcgauacca caucuauaag     2940

aaccgcgaca uuaaugauuu ucaucacgcc caugacgcuu acaucgccac cauucuuggg     3000

acuuauaucg gacaccgguu cgaaucccug gacgcaaagu acauauaugg ugaguacaaa     3060

agaauuuuua ggaaccaaaa gaauaaaggc aaggaaauga aaaagaacaa cgauggguuc     3120

aucuugaaua guaugcguaa caucuacgcg gacaaggaua ccggagagau ugugugggac     3180

cccaauuaua uagaucgcau caaaaagugu uuuuacuaua aagacugcuu cgugacaaag     3240

aaacucgaag agaacaaugg caccuuuuuc aacgucacug uucugccaaa ugauacaaac     3300

agcgacaagg auaauacgcu agcuaccgug ccugugaaca aguaccgguc uaauguaaac     3360

aaauauggug gguuuuccgg cgucaauuca uucauugugg ccaucaaggg caaaaagaaa     3420

aagggaaaga aaguuauuga ggugaacaag cugacuggga ucccccucau guacaaaaau     3480

gcagacgaag agauaaagau caacuaucug aaacaggccg aagaucuuga ggagguccag     3540

auuggaaagg aaaucuugaa aaaucagcug auugagaagg acgguggcuu auacuacauc     3600

guggcuccaa cagaaauaau uaacgccaag caacucaucc ugaacgagag ucagaccaaa     3660

cuuguuugug aaaucuauaa ggcaaugaaa uacaagaauu augauaaccu ggacagcgag     3720

aaaauuaucg accuauacag acuccugaua aauaagaugg aguuguauua ccccgaauau     3780

aggaagcagc uggugaaaaa guuugaggau cgauacgaac aauuaaaagu aauuucgauc     3840

gaggaaaagu gcaacauuau caaacagaua cucgcuacac ugcauuguaa uuccucuauu     3900

gggaagauca uguacagcga cuucaagauc ucaacuacca uuggacggcu uaacggccgc     3960

acgaucaguc uggaugacau uuccuucaua gccgagucuc cuaccgguau guauagcaaa     4020

aaguacaaau uguga                                                      4035


<210>  2996
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2996
augaagaaaa ccaaccccua cauccugagc cucgacauug gcacagccuc cauaggguau       60

gcuugcaugg aucuugacuu caauauccug aaguaccacg auaaagacgc aauuggagug      120

uugacuuuug agggugccga uacggcggcu gaaagaaggg gcuuccggac cucucgccga      180

cguaagaaca gaaggaucaa acgguuaggg cugcuacagg agguccucgc cccucugguu      240

aagaauccaa acuauuacca acuucugaau uugcauaagu ggaaaaacuc aaaugaagac      300

uuuaagcacc gcagucucag cgaggugcug aaaauucugg gaaagaaccc gaaaacauau      360

cccacuaucu accaucucca gcaggauaua cagaaucacc cugaaaagaa guucgagcca      420

gaguuaaucu uucuggcacu uuaucauuug guaaaauaca gaggccacuu ccuguuuaac      480

aaucuauccu ucgacaaggg uaaaaccgug gauaacgaag cccaacucuc ugagcugcuu      540

gaacaguacc agucgauuaa ucccuaugag gacucaauca gcaacaagga uauuaauguc      600

aucguggaca cacugaaaga ugaccaguug accaggaacg acaagaguaa agagcucaag      660

cggaacguuu ccaagacuca cgcugaacug uuuaaaaugu uaguggggcu gaaguucaaa      720

caaucuauac ucuuugagaa uauggaaaac gcagaggaua uuaaggaaag cgccacagga      780

uccgacgcua ucucacuuag ugaugacuac uaugagaauc ugagcgaguu cuugaccgau      840

gaacagcuaa acuuuauuga gucugccaau acguucuuca ugggcaucau gcuggaccgc      900

auccucaaag gcgcgacuuc cauuucagca gccaaggucc gagauuacaa gauauuuaaa      960

aaccagcuga agaucauuaa agacaucgug uaucagaaua aguacgauaa auaucaugag     1020

uucuuuguaa ccagcaagga ggacaugaga gcuuaccaag aaaacccuga ugccaagaca     1080

uucgggaaac uuaguaaguu ugaccaguac cugauacacc caaaagacca guaugcaaag     1140

uugaugaaag aucucaaggg aauuguuccc aagaaauaca cugaggugcu gaauuuaauc     1200

gacgagcaaa aguuccuggu cgugcagaac accacagaua augcuucuau cccuuaucag     1260

aacaaugugu uugaagccga gaacauucua aggaaucagc aaaaauacua uccagaaauc     1320

accgaggaaa ugauugguaa ggucauaaac cucaucuccu uccggauucc guacuauguu     1380

ggcccccuga cgaacaaaaa ugggcagagc gacuuugcgu ggaugauccg uaagucggau     1440

gagcagacuc uuccuuggaa cuucgacaaa gugaucgaua agucuaaguc cgccgagaaa     1500

uuuauucgca gaaugacauc aaaguguacc uacuugcugg aagaggacgu acucccaaaa     1560

cauagucugc uuuaccagga aauggaggug cugaaugagu ugaacaaugu ccaacucagg     1620

ggagaaaacg agcccaagca ccggauacac cgacuggauc cuaaagugaa gcaguucauc     1680

uaucauaauu uauuucagaa gaaaaagaac guuacucaca aagaccugau uacacuacuc     1740

cagaauagcg aacaucgcaa cacccugaau aagacguccg gccaaaaacu uaagguguuc     1800

gguacccaga acgauucuaa guucgcaagc aaauugucaa cauacaauga caugaaggcu     1860

aucuuuggga gugucgagaa caaaagacag augauugaag aacuggugcu cuggaucacu     1920

auauucgagg auaaggccau ccugaaacag aagauuggag aaaaguaucc aaauaucacc     1980

gagaaacaau uaaaccagau uguaaagcug aacuacuccg gcuggggcag gcuuagcgaa     2040

aaauugcuca caaaggaggu uaaugggcgg ucucugaucg accuaaugua uucaacugaa     2100

aacaauuuua uggagauaau uaccgacaaa acacugggau ucgauugcuu uaucaagaac     2160

aguaauaaag ugaagaacgc uaagauuagc uacaaagacg ucgccgagcu ccgcaccucc     2220

cccgcacuga agaaagguau cuggaaugcc auaaagaugg ugagagaaau cucugaucuu     2280

uucggcgcuc cggagaaaau uaucauugaa uuugcgacug aggaccaggu caaggggaag     2340

aggcaaaaau cgcguagcga auugugggau gaccugguga agaaaaacaa uuuaaagcgg     2400

aacaaaaagu ucaagggacu cuuugaggag cugaaagcau auccugaucu ugacuucucc     2460

aaucccaagc ugugguugua cauccaccag aacggcaaau guauguacac gaagaaacca     2520

auagaucucg aacgacugau gucagacaca aauaaccagc uauaugagau ugaucauauc     2580

cugccuagaa ccuuuguuaa ggacgauagu aucaauaaca aggugcucgu aauucagucu     2640

gccaaccaga aaaagagcgg ugacaaaaug ccccuggaaa ucuucgacaa ucacguccuu     2700

gugccauuuu ggaagauguu guacgagaac gaucugauuu ccucaagcaa auuaucuaag     2760

cucaugaaga aagaguucaa ugaccuggau aaggaagggu ucauacaaag gcagcuuguu     2820

gagacucgcc agaucauuuc ccacgugagu gacuuucuga ccgaagagua ucccagcaca     2880

accgucgugc cuaugaaagc uaacguagug ucugaacuac ggagaaaguu cgauauccca     2940

aaaauuaggc aacucaauga cuggcaucac gccguugaug cauaccugaa cggcuuuguc     3000

uaucaggccg cucgcguguu cuacccgaag gugaauuugu uugaguucaa cuuuaaaugg     3060

gagaaggucc gagaaaagug gcggcagcug ggagaguuca cugaaaauaa caaaaagcgu     3120

gaguuuuucu uauuuaaaag aauggaaaau uauguuaacg agcauggcga ggggaugauc     3180

uccaagcuca uauucgaccu ggauaauuuc aaauuuaacu acucaaggaa gguggaaagu     3240

acagagagcg ccuucuacaa gcagucggca uuuuccccca aaaauacgac cgcgcaauau     3300

gacucugaua agaacaaaac ugcuaucuac aaggaaauga aaaauaagcg cggaauugug     3360

guaagcuaua cauacgucaa gaaagaccag gagcuuaucg guuauggcgu guucaacgaa     3420

accguuuuug aguacgugaa caaugccgac gucgauauga acacacgggc acugaagauu     3480

gugagaaaag ccuucccuaa guuggacauc aaaaaggcuc aguaucucuu uucaauaaau     3540

aagggggaug cccugcuagu uaacggacac ccauuccugu uugugagugc aaaagagagg     3600

auuaauggca agcaacuuga guucgacauc aacaugcagc gacgccucaa aaaggcucug     3660

cggcccuaca auaaaaugac ugaacaggaa aagguaggug ucuacaacca guuugccgag     3720

aaaguggugc aagaguauga aaauuccuug ggggcgaaga ccagaaacga gaagcuggaa     3780

uuaaucagga aucaguucaa cgagucugau aaaagcaaug aagcccucau ucagugcaua     3840

cuggaggucc uuaagaugac gaaaguuucc gcugagcguu cagugaagcu gaaaauccgc     3900

uggggcagcc agaccccuaa cacauugauu gacaucaaua acaaggcaaa gauuauguac     3960

aacaguauca cuggacucaa acaaacccgg cugaagucua uauauaaacu agccgaaucc     4020

aagaaugagc uguga                                                      4035


<210>  2997
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2997
augaacaaga auuacuauau cggccuggac auggggacca acagcguggg augggccguc       60

acagaucagg aguacaaucu cauuaaacuu aaggguacuc acgcuugggg cgcaagaaua      120

uuccaagaag ccaaagacaa gaaagggacg agggcgaucc gguccucucg ccgacguaug      180

gauagaagga aguaucggau ucaucuguug aaccaguuau uugcucagga gauccaggcc      240

auugacucaa ccuucuuuau ccgccuggca uacagugccu auugggaagu ugauaagaaa      300

gacaagggaa uaggcagaaa uauccuauuc aaaacaaagc aagaggaaag ccaguuuuac      360

aaaaaguauc ccacuauuug gcaccucagg aagucccugc uugagaacca gccugaugcu      420

uucucugacc ugcgguacgu guacuuggca uuucaucaca ucaugaaaua ucgcgguaau      480

uuccuccaug agggggaaau uaagaccaac gagcugcagg uacaacagau cgaacagcug      540

aaucaguuuc ucauaaacaa auacaaggag auuuaugaua augacgugga ggaacgaaca      600

uucaucgcca accaccaaau ugaugcuuua aaagagaucc ugcuuaauaa gaacaccaau      660

aaaacuacaa aggaaaagga gaucaaaaag uuguuugacu acgccgauug cgaaucgcug      720

gagccauaua uuucauguuu cagcaaacua gcggucggag gcaaguuuga ccucaguaaa      780

cugggcuacg aggggaacau acaguucucc gacccgucuu ucgaagagaa ggcaagcauc      840

cuucaggaug acuuuagacu gauugccauc gcuaaggaaa uguaugauuu cgugauauug      900

aacgaccucc ugaaagauca acccaauuua uccaccgcca ugguucacgu guacgagacg      960

cauaggcagg accugaagaa acucaaggau auuaucaucg acauugaugc aaacaaagga     1020

cuucagaaua acgaccggug guacuuuaag cuguucaagg auaaaaaugc uccuaagaac     1080

uauucagccu uuauccacaa aaauaguaac cagaagcguc caagcauuga cgaguucaau     1140

gacgaaauca aaaagauauu gauugagcau cgcaacgaug ucucucuaga aaagcaagac     1200

gaucugaaaa ugcucuuuga gaagguggaa aauaaaacuc ugcuuaagac caucgcgauc     1260

caguccacau caguaauucc ccaccagcug cacuuggacg agcucgagaa aauaaucgau     1320

aacgccagca cuaaguaccc uuuccugagu aauauuagag acaaauuagu ucugcuauuu     1380

accuucaggg ugccauauua cuaugguccc cucaacgaua agucuccuua cuccaauguc     1440

gaacggaagc aguuugcaac agugacccca uggaacuucg acgagaucuu ugaugaaaac     1500

aaaacgcgag agaaguucau gaaaaagcug acuaauaacu gcacauaucu uagcggcgaa     1560

accgugaugc cgaaaaaggc uuuggccuac gaggacuacc ugauuuauga ucgccucaau     1620

guccugcuug uuaacgggga guaccugucg uugcaagaaa aggagaaaau cuaucauuac     1680

cucauaucua gacccaagac uacacuggac cagcugaaaa aggaauuagc uaaauggcac     1740

aaucaguccc cuaucgauau uaucuucuca aacauucaug aggaaauccc auuugaggcc     1800

aguagccacg cacaucuaau gaaguccuuc gaccucgaga agcagaaaga ggaacuggaa     1860

agguauauaa cccuugccac gauuuacgcu gacgauaaga aaucuuugaa ggagcugcuc     1920

aaaaaugaaa gccuguuaga cgagaaccaa aagaauguga uccugacccu ucccacaaag     1980

aaaugggcgc cuuugucaua uaagcuccug cagaacauug uauacacuga ugacuacgga     2040

gugguccaca guauccuaga uauaaugaaa gagaccaauc agaacuuuca gauggugcug     2100

cacaaugaac aauaucauuu caaggagcuc aucgaacagg agaaccagaa auuuacacaa     2160

gaaaagacua uugaggaccu gaucgaggaa aaacuugagu ccauucccag cuugacccag     2220

cggucuaucc gccagacacu guuaauaauu gaugacauca ucaaggcauc acaccagaag     2280

guuccagaga aaauuuucau cgaagugacc agaaauaacg augacaagag gaaaggccaa     2340

gaugguaacc agcucgcccg ugacaaggag cugaaucggu uucuuacuaa ccuguugaaa     2400

gaugaaaagg cuaaucaguu cugugacguc agucagcucc ugaaggaccu agaggaagug     2460

gauaaaguaa agcugaaagg gaagcauauu uaccucuauu uuaaacaacu gggaauagac     2520

cuuuacacgg gcaagaagau cguuuugaac cagcuguuaa caagcaccaa auaugauacu     2580

gaccacauua ucccgcaguc ccucauuaag gaugacucuc uggauaaucu ugugcugguc     2640

gagcgagaag ugaaccuaaa agucaagggc aaucacuacc cugugcccuc gagaauccag     2700

gacgagcaga aaaagcucug gcauuuccug uugcaaaaga aagccauaag cgagaagaaa     2760

uaugggaacc ugaccaggac auccaagauc aaauuagaag agguugaaga uuuuguggca     2820

cgccagauua auguaaucaa cuacucaaau gucauuaucc gggacauacu caagcugaag     2880

uacccacaca ccgauauuau cuucaguaaa ucucaauauc cuagcuuuau cagaaagcau     2940

cuuaaaauug ugaagaacag ggacguuaau gacgcccacc augcugugga ugccuaccug     3000

aacgucgugu ccggaaauuu gcucucaacu acauucagca aagcauauau acgcaagaaa     3060

uacauccaga uucaggacgg ugagaaggaa acguucaaca uggagaccau ccuggagaac     3120

uauuuuaaga auaacgaaaa ucagcuaaaa auuaagacua acugccugcg acgggaugcg     3180

cuuaucacau ucaaaguaga cuacuuuucu ggcgaguucu augggcaaac ccgucucccc     3240

aagggaaaug aaaaccugau accaaucaca uugaaugagg auaaccccau guccgacacu     3300

ucaaaauacg gcgguuacac caaucuggcu caggccuauu uauuugcagu gagcuaccag     3360

gaagaugggg agaugaagaa ggccauucuc agaguuccuu aucuguacga cacgcuuuau     3420

aaaaacgauu cugacaagcu guuguccaaa guccucgaua auccaaaggc uaccaacauc     3480

cagauucuga ggaaaaucca ccuaaaucaa aagauacgcu ucaacgacgg cauuuaccug     3540

cuuuauacaa augagaagga ucggaacaaa uacaagaugg ccuaccagaa uuauaucgac     3600

aacgacuacc ucuauuaccu gcacugggca aacaaacauu uggaagaguu acuggauaau     3660

caggaaaagc agacuuuuau cuauaacagu aaaaaugacc aauucgaggu gaguaaggcu     3720

aagaacaaag agauuuuuca guaccuccug cagaagacca aagccaaggu guaugauaca     3780

uguaauuaca ucgucaaagc gagagaaacu aacauagaga uuuucgaaca gcuuacccug     3840

aaggagcaaa ucacgaugau uaaucagugg aucaaaauga ugagcaggga cugcgaaaag     3900

guuaaguuug augcccgauu cacaaacuuc ucgaauuguu ugcuacgccu guccaacaau     3960

auaaacccga aagacucuau uaccaucauc uacgagagcc ccacuggacu cuuuaagcac     4020

gaggugaaaa uuuga                                                      4035


<210>  2998
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2998
augaagaaaa ccaaccccua cauccugagc cucgacauug gcacagccuc cauaggguau       60

gcuugcaugg aucuugacuu caauauccug aaguaccacg auaaagacgc aauuggagug      120

uugacuuuug agggugccga uacggcggcu gaaagaaggg gcuuccggac cucucgccga      180

cguaagaaca gaaggaucaa acgguuaggg cugcuacagg agguccucgc cccucugguu      240

aagaauccaa acuauuacca acuucugaau uugcauaagu ggaaaaacuc aaaugaagac      300

uuuaagcacc gcagucucag cgaggugcug aaaacacugg gaaagaaccc gaaaacuuau      360

cccaccauuu accaucucca gcaggauauc cagaaucacc cugaaaagaa guucgagcca      420

gaguuaauau uucuggcacu uuaucauuug guaaaauaca gaggccacuu ccuguuuaac      480

aaucuauccu ucgacaaggg uaaaacagug gauaacgaag cccaacucuc ugagcugcuu      540

gaacaguacc agucgaucaa ucccuaugag gggucaauuu uuaacaagga caucaaaguc      600

auuguggaua cccugaagga cgaucaguug acuaggaaug acaaaagcaa ggagcucaag      660

cggaacguua guaaaacaca cgcugaacug uucaagaugu uagugggacu gaaauuuaag      720

caauccaucc ucuucgagaa cauggaaaau gucgaggaca uaaaagaauc ugcaaccggc      780

agcgaugcca uuucccuuuc agacgauuac uaugagaacc ugagugaguu uuugacggac      840

gaacagcuaa auuucaucga gagcgcuaac acuuucuuua ugggcauuau gcuggaucgc      900

auccucaagg gggccaccuc uaucuccgcg gcaaaggugc gagacuacaa aauuuucaag      960

aaucagcuga aacuuauaaa ggauaucgua uaucagaaca aauacgacaa guaucaugaa     1020

uuuuucguua caucaaagga ggauaugaga gccuaccaag aaaauccuga cgcuaaaacu     1080

uuuggaaagc ugagcaaauu cgaucaguac uugauucacc caaaggacca guaugccaaa     1140

cucaugaagg accugaaggg uaucgugccc aaaaaguaca ccgaggucuu aaaccugaua     1200

gaugagcaaa aauuucuagu ggugcagaau acaaccgaca acgcaaguau uccuuaucag     1260

aauaacgucu ucgaagcuga gaauauccuc aggaaccagc aaaaguacua uccagaaauc     1320

acggaggaaa ugauuggcaa aguuaucaau cugauuucuu uucggauacc guacuaugug     1380

gggccccuua cuaacaagaa cggacagucc gauuucgccu ggaugauccg uaaaagcgac     1440

gagcaggcgu ugccuuggaa uuuugauaag guaauugaca agucgaaauc ugccgagaag     1500

uucauccgca gaaugacauc caaauguacc uaccugcucg aagaggaugu gcugccaaag     1560

cauucacuuc uguaccagga aauggagguc uugaacgagc ucaauaacgu gcaacugagg     1620

ggcgaaaaug agcccaaaca ccggauccac cgauuagacc cuaagguuaa gcaguuuauu     1680

uaucauaacc uguuccagaa aaagaaaaau gugacucaca aggaucuaau aacacuccug     1740

cagaacagug aacaucgcaa uacccuuaac aaaacgagcg gucaaaaguu gaaggucuuc     1800

gggacccaga augacuccaa auuugcaucu aagcugagca cauacaacga uaugaaagcu     1860

aucuucggau caguggagaa uaagagacag augauugaag aacucguacu guggguuacu     1920

aucuuugagg acaaagccau uuuaaagcag aagaucggcg aaaaauaucc aaacauaacc     1980

gagaagcaac ugaaucagau cgugaaacuu aacuacagug gcugggggag guuguccgaa     2040

aagcuccuga caaaagaggu caacggacgg agccuaauug aucugaugua uucuacugaa     2100

aauaacuuca uggagaucau uaccgacaag acacucgguu uugacugcuu caucaaaaau     2160

ucaaacaagg ugaagaaugc uaaaauaagu uacaaggaug ucgccgagcu gcgcaccagc     2220

cccgcacuua aaaagggcau uuggaacgcc aucaaaaugg ugagagaaau uuccaauuug     2280

uuuggggcuc cggagaagau cauaaucgag uucgcgacug aggaccaggu uaagggaaaa     2340

aggcaaaagu cucguucgga acugugggau gacuuaguga aaaagaacaa ucucaaacgg     2400

aacaaggagu uuaagggccu guucgaggaa auuaaagcau auccugaucu ugacuuuagc     2460

aaucccaagc ugugguugua cauccaccag aacgguaaau guauguacac gaagaaacca     2520

auugaucucg agcgacugcu auccgacaca aauaaccagc uguaugaaau cgaucauaua     2580

cucccuagaa ccuucguaaa ggacgauuca auuaauaaca agguccuggu gauccagagu     2640

gccaaccaga aaaagucugg ggacaaaaug ccccuugaga ucuuugauaa ucacguuuug     2700

gugccauucu ggaagaugcu guacgagaac gacuuaauua gcuccucaaa acucagcaag     2760

cugaugaaga aagaguucaa ugaccuugau aaggagggcu uuauccaaag gcagcugguc     2820

gaaacucgcc agauuauauc ucacgugucc gacuuccuaa ccgaggaaua ucccaguaca     2880

accguagugc cuaugaaagc uaacguuguc agcgagcucc ggagaaaguu ugauauccca     2940

aaaauuaggc aacugaauga cuggcaucac gccguggaug cauacuugaa cggauucgug     3000

uaucaggccg cucgcgucuu uuacccgaag guuaaucugu ucgaguuuaa cuucaaaugg     3060

gaaaaggugc gagagaagug gcggcaguua ggcgaauuua cugagaauaa caaaaagcgu     3120

gaguucuuuc ucuucaaaag aauggaaaau uaugugaacg agcaugggga aggaaugauc     3180

ucuaagcuga uuuucgaccu ugauaauuuu aaauucaacu acuccaggaa gguagaguca     3240

acagaaagug ccuuuuacaa gcagagcgca uucucgccca aaaauacgac cgcgcaauau     3300

gacuccgaua agaacaaaac ugcuaucuac aaggagauga aaaauaagcg cgguauaguc     3360

gugucuuaua cauacguuaa gaaagaccag gaacugaucg gcuauggggu guuuaacgag     3420

accgucuucg aguacgugaa caaugccgau guugacauga acacacgggc auugaagauu     3480

gugagaaaag ccuuuccuaa gcucgacauc aaaaagguac aguaucuguu cagcauuaau     3540

aagggagaua cucuacuggu caacggccac ccauuucuuu ucgugucagc uuacgaaagg     3600

aucaauggua aacaacucga guuugacaua gccaugcagc gaaagauuaa acaggcacug     3660

agucccuaca acaauaugac cgaacaggag aagguggagu ucuauaacca auuugcugaa     3720

aaagucaucg aggaguuccg caauuccauc ggguugcagu cucgggauag aaagguuaac     3780

cugauuaaaa auuuauucaa caauacggaa aagagcaacg acuccaugac ccagucaaua     3840

cucgaagugc ugaagaugac aaaaguaagc gccgaaagga guaaggagcu uaaaauccgu     3900

uggaauggcc agacuccuaa caccuuuauu gauacaaaga acaaugcgga aauccaauac     3960

aaaucuauua cuggacugaa gaccacgaag ccaaaaucca ucuucaaguu ggccgagagc     4020

aaaaacgaac ucuga                                                      4035


<210>  2999
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  2999
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

guuacagaug acuauaaggu gccugcuaaa aagaugaaag uacucgguaa uacugauaag      120

ucucacauaa agaaaaaccu ucugggcgca uuguuauucg acucagggaa uacggccgag      180

gauagaaggc ugaagcggac cgcgcgccga cguuacacaa gaaggcggaa ccgcauccua      240

uaucuccagg aaauuuuuag ugaggaaaug ggaaaagugg acgauagcuu cuuucauaga      300

cuggaggacu ccuuccuugu cacugaagau aagaggggcg agcggcaccc aaucuuuggu      360

aaucuggagg aagaggugaa auaccaugaa aacuucccga ccauuuauca cuugcgccaa      420

uaccucgcug acaaucccga gaagacagau cugcgacugg uuuaucucgc cuuagcacau      480

aucauaaagu uugggggaca cuuccugauc gagggcaaau uugacaccag aaacaaugau      540

gugcagaggc uuuuccagga auuuuuggcc gucuacgaca acacuuucga aaauucuucg      600

cugcaggaac aaaacgugca gguagaggaa auucuaacag acaagaucuc aaaaagcgcu      660

aagaaagauc ggguucucaa gcuguuuccu aaugagaaaa guaacggccg uuucgcagag      720

uuccuuaagc ugauuguggg gaaucaggcc gacuuuaaga aacacuucga auuggaggaa      780

aaggcuccac uccaguuuuc caaagauauc uacgaggagg agcuggaggu cuuacuggcc      840

caaauaggag acaacuaugc ggaacucuuc cuuucugcaa agaaacugua cgauagcauu      900

uugcuauccg guauccugac cgugacggac gugucaacua aggcaccccu cagugcuagc      960

augauucagc gcuauaacga gcaucagaug gaucuggccc agcuuaagca auuuaucaga     1020

cagaaacugu cugacaagua caaugaaguc uucuccgaug uuucaaaaga cggcuaugca     1080

ggguacaucg auggaaagac caaccaggag gcuuuuuaua aauacuugaa gggccuccug     1140

aauaagauug aagguagcgg guacuucuua gacaaaauag agagggagga cuuucugcgg     1200

aagcaacgaa cauucgauaa cggaaguauc ccucaccaga uucaucuaca ggaaaugcgc     1260

gccaucauaa gaaggcaggc ggaguuuuau ccauuccucg ccgacaauca agaucggauu     1320

gagaaaaucc ugacuuuucg cauccccuac uaugugggcc cucuugcaag aggcaagucu     1380

gacuucgcuu gguuguccag gaaaagcgcc gauaagauua ccccauggaa cuucgacgaa     1440

aucguagaua aggagucguc uguggaggcu uuuauuaauc guaugacaaa cuacgaccug     1500

uaucucccga aucagaaagu ccugcccaag cacucccuuc uguacgaaaa auucaccgug     1560

uauaacgaau ugacgaaggu uaaauacaag acugagcagg ggaaaacagc cuuuuucgau     1620

gcaaauauga agcaggaaau auuugacgga guguucaagg ucuaccggaa agugaccaag     1680

gauaaacuca uggacuuucu ggagaaggag uucgaugaau uucgaaucgu agacuuaacu     1740

ggucuggaca aagagaacaa ggccuucaau gcuucauaug gcacauacca cgaucuaaga     1800

aagauucucg acaaagauuu ucuggacaac aguaagaaug agaaaauccu ugaagauauc     1860

guuuugaccc ugacccucuu cgaagacagg gagaugauuc gcaagcggcu gaaaaacuau     1920

agcgauuuac ugacgaagga gcaacuuaag aaauuggaaa gaaggcauua cacagggugg     1980

ggacgccucu ccgcggagcu gauacacggc auccgaaaca aggaaucucg gaaaacuauu     2040

cuagacuauc ugaucgauga cgguaauagc aaccguaauu uuaugcagcu cauuaacgau     2100

gacgcacugu cauucaagga ggaaaucgcc aaagcucagg ugauagggga gaccgauaau     2160

cuuaaccagg ucgugaguga caucgccggc uccccugcaa uuaagaaggg aaucuugcaa     2220

agccugaaaa uugucgacga guuagugaag aucaugggcc aucagccaga aaauauaguu     2280

guggagaugg ccagagaaaa ccaguucaca aaucagggga ggcgcaacuc ucaacagcgg     2340

cucaaaggac ugacugauuc aauuaaggag uuugguaguc agauccuuaa agagcacccc     2400

guagaaaaua gccaacugca gaacgacaga uuguuccucu acuaucugca gaauggcagg     2460

gauauguaca ccggggagga acuagacauu gauuaccugu cccaguauga caucgaucau     2520

auaaucccuc aggcuuuuau uaaggacaac ucuaucgaua aucgaguccu cacaucgagc     2580

aaagagaacc gcggaaaguc cgacgaugug ccaucaaaga acguugugcg gaaaaugaag     2640

aguuacuggu cuaaacugcu uagcgccaag uugauuaccc aaagaaaauu cgacaaucug     2700

acuaaggcag aaaggggcgg uuuaacggac gaugacaagg cgggguuuau caaacgucag     2760

cucgucgaga cacgccagau aaccaagcac guggcucgga uucuggauga gagauuccac     2820

acugaaaccg acgagaacaa uaaaaagauc aggcaaguaa aaaucgugac acuuaagucc     2880

aaccugguuu caaauuuucg aaaggaguuc gaacuauaua aaguccggga aauuaacgau     2940

uaccaucacg cccaugacgc auaucucaau gccgugaucg gcaaggcucu guugggagug     3000

uacccccagc uggagccgga guuugucuau ggcgauuacc cucacuucca cgggcauaaa     3060

gaaaacaagg ccaccgccaa aaaguuuuuc uacagcaaua uuaugaacuu cuuuaagaaa     3120

gacgauguuc gcacugacaa gaauggagag auaaucugga aaaaggauga acacauuucu     3180

aacaucaaaa agguguuauc cuauccccag gugaauauug uaaagaaagu cgaggaacag     3240

acagguggcu ucaguaagga gagcauccuc ccaaaaggga acucugacaa gcugauaccu     3300

agaaaaacga agaaauuuua cugggauacc aagaaguaug gaggcuucga cucccccauc     3360

guggcuuacu caauucuugu uaucgccgac auugagaaag guaagaguaa aaagcugaaa     3420

acugugaagg cguuggucgg ggugacaauc auggaaaaga ugaccuuuga gagggaucca     3480

guugccuucc ucgaacggaa aggcuaucga aaugugcaag aggagaacau aauuaagcug     3540

cccaaauaca gccuauuuaa gcuggaaaac ggaagaaaac gccuucucgc uucggcaagg     3600

gagcugcaga agggcaauga aaucguauug ccuaaccauc uggggacauu acucuaucac     3660

gccaagaaua uccauaaagu cgacgagcca aagcaccugg auuacgugga caaacacaag     3720

gaugaguuca aagaacuucu ggacgugguc uccaacuuuu cuaagaagua cacuuuggcu     3780

gagggaaauc ucgagaaaau uaaggaacug uaugcccaga acaauggcga agaucuaaaa     3840

gagcuggcaa gcucauucau aaaccuucuc accuuuacgg cgaucggugc cccggcuacc     3900

uucaaguuuu ucgacaaaaa uauugaucgu aagcgguaca caaguacuac cgaaauccug     3960

aacgccacau ugauucauca auccaucacu ggguuauaug agaccagaau agaccugucu     4020

aaacucggag gcgauuga                                                   4038


<210>  3000
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3000
auggagaacu acaauaucgg ccuggacauu gggaccacaa gcgugggaug ggccgucguu       60

aacgaugcua cuaauaaggu gaugagaaaa gguggcaagg cacucugggg aguaaggcuu      120

uucgaccccg ccgugacggc ggaagaucgg cgcgcuaaac gagggacccg uagaagguau      180

gaccggcgca gagagaggau aaagcuguug caggaugaau uuaaagacga gaucaacaag      240

gucgauucca auuucuuuuu aaagcuggaa gagucuaaau acacaucaaa ggacauuaaa      300

aacaagacug ugcuaaucag uauggaggaa gagaaagcca uuaagaagua uaaucaagaa      360

uacccuacca ucuaucaccu ccggcagaaa cugaugaaca gcaaugagca gauggauaua      420

cgccuuguuu accuggcaau ccaucacauu aucaaguauc gaggcaacuu cauuuacgag      480

gguaauuccu uuucugugaa caauuugaac aucgacaaua aacucaagga aauauuccug      540

ucgcugucag aggaugucga cgaacucgau auuagcgagg aaugcaucaa caacauuaau      600

uacaaacagu uagccaacau cuuuaaugag caaaguaaga acgaccugaa agugaaacuu      660

acauccgagu ugaccucuua uagccugaau aagaacuuca ucuccgaauu uuguaaacua      720

auuauaggga auaaguucuc agcuaaaaag cucuuuaacc uggagacuga caaugaugua      780

agucuuagcu ucgacggaac agauuuugaa gacaaagcag gcgaucugga gucuuugcuc      840

aacgacuacc uggaaguuuu agagcagcug aaggagcucu augauauggu guuccuuaag      900

aaaaucuucg gcggguccaa ggaaaccacg auuucaggac ugauggucga gagauacaau      960

cagcauaaaa aggacuugaa acuacugaag gauacuuuua gcagugacag gaagaucuau     1020

aacaaaauau uccggucuaa gaaagauauu aaggacgaca aagauaugug ccucuacgac     1080

cuguauaucc acaauaaacu ugaaaccaca gauuuucuga aggagaucaa aaaguacgug     1140

aacacugaca auuucauuau caccgaugaa gacuuuuuga aacgucucga gaacaacgag     1200

gugcugccac gcauuacauc caaggaaaau gguaaauacc cguaucaguu aaacaaggau     1260

gagcugguca gcauaaucga acaucaaggc aaacacuacc ccuuccuacu caauaagacc     1320

gacgauggga uuuauaagcu gguuagacuu uuggaguuua ggaucccuua cuucguggga     1380

ccacugguau cgaaagacau gucugagaac gccuggaugc uccggaauga aggcaugcag     1440

ggugugcaca ucacgcccua uaacuuuaag aaagucauua aucuggauaa gacugcugag     1500

auauucauca aacgaaugau uucccauugu acauaccuuc uggacgaaga ggccuugccu     1560

aacaauucaa uccucuauag ugaauuuaag gugcugaacg aguuaaagca gauuaaaguu     1620

aauggggagc accugaccaa ggaugaacag cauaaaauca uagaggaacu auucaugaag     1680

acuagcggaa caaucaccga gaacaaauuu cgcgaguucc uccugaauac gcuugacuac     1740

aacuucuacg aaaaugaguu gaagauuacc ggcuauuccg cggacugcaa guuugcaaac     1800

aacaugcaau cuuacaucga uuucuuuggc gaaaauggga uuuucgaggg aacaagcuau     1860

aacacuaaug acgccgaaaa gaucauagag uggauuacca ucuuugauga caaggauauu     1920

cugaaaagaa agguggaaaa cgaguaccca gagcucaaau caaaucaggu caagaacauc     1980

cugaauaaga aauauagugg uuggggcagg uuaucccgga agcugcuuuu ggaccuccgc     2040

gugcuggaua aagaaacaga cauacccaag agcaucaugg aucuaaugua cgagacugac     2100

gaaaacuuca ugcagauuau caauaacgau aaauauaauu uucaaaagcu gauugcuaaa     2160

gagaacaaga ucgagaauac cucuaacgac cucucauacg auguaguuga aaaucuggcc     2220

acaaguccug caaccaagag agggauauac caggcucuua aaguggucaa ggagauuacu     2280

acguauaugg gauacgaacc gaaaaacauc augguggaga uggccagggg cgacgaaaag     2340

aaaauccgua aggacgaucg gaagaaauau uugcagaagc uguacgacaa cauuaaagau     2400

gaggucgaca gcaauuauaa gaacuuaaag aaggagcucg cgaaggaaga gaaaaucgau     2460

acagacaagc uguaccuuua uuaccugcag gaagguaaau guuuguacug cgggaagccg     2520

auuaaugugg augaccucaa caccaaaaau ucccuguaug agauagauca cauccuaccc     2580

cgaacucuga uuaaggacaa cucuugggau aauagaguuc ucguguguag gggcugcaac     2640

caaaagaaag ccgcaucgcu gguacuuccu aaggaguucc gcaaugacua caccaaaagc     2700

aucuggauuc gguugaagaa caauaaacug auguccaaca agaaguuuua uaauuuaaca     2760

agaaaaucau acagugaaga ggacaucaag ggauucauaa acaggcagcu cgucgaaacc     2820

cgccagauca cuaaacacgu ggcuaauauu cugaacuguu uuuauccaaa gacaaaaauc     2880

guuuaccuua aggccaauau uucucauagc uaucgagaua aauacgaccu guucaaguuu     2940

cgggauauca acgacuacca ccaugcucac gaugccuauc uagcagccac gcucggcucc     3000

uaccaggaga aguaucugca uuacgacuug gauuauaaca aaauaaauga guucaacgaa     3060

aagcugauua aaaaugaaga cuacaagcgu uuacacuaug ggaucguggu caacucacuc     3120

gaugaggagu uccaaagcgc uuuugcggaa aucgcaccca gacugauuaa ugaggaaacc     3180

ggagagcuuc uguucaacgc caaaaaguuu aaugacuugg uggaggauac ucucuacagg     3240

aacgacauac ugaucucucg caagacagaa auucggaccg guaaacuaua caaggagaca     3300

aucuauaaaa gaggcaaggg gaauauuaaa aucaaggaaa acaugccuac ugagaccuac     3360

ggaggcuauu ccaauaugga aacgaguuac cugacccuug uaaaguaugg uaacaaaaca     3420

aagcucauag ggaucccaau gagcauugcu aauaacaaag augugaaggu uaaacuggac     3480

uucaucaagg agcacuugga ccugaagucu uuagauaaua uugucauccu caaagacaac     3540

auacccuacg auguguccau uaacuauaag aaucaggacg uguacgucaa aggcuacuca     3600

acugccaaca agaauugcga gcugaguaac gcacaucagc uuaaaaucaa gaaagauaug     3660

augaagaagu guaaauaugu ucugaauaag guguacaaaa acaaguauga cgauaucacc     3720

aauauggaca uugaaaacgc caucacauuu auaaauuacu uguucgagau uaaaaucgaa     3780

uuuccacucu ucagcaagga gauuacuaag auccaggaua acauaaaucu gaccgaaugc     3840

ucguuugagc aacuagcuaa cauuaucaaa cagcugcuua ucauuuauca cugcaauucc     3900

aagaacggaa aucucaaaga guucggccug ucugacagga ucgggcgauu gagcggagcc     3960

aacgugacgg guggcgauuu uauuuucaca ucaguaaccg gguuaaagga gaaaacugca     4020

aguuacgacg gagaauga                                                   4038


<210>  3001
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3001
augguggaga aguacggcau cgggcuggac auuggaacca gcacacucgg uuggacuucc       60

gucgaugaaa acggcaaaau acagagacuu aaggggcaaa cggcccuggg aaccagguug      120

uucaaugagg gccagacagc ugaagaacgg cgcgguuuuc gaacuacccg uagaaggauc      180

cagcggcgca gauggagguu acggcugcua cgcgaaauuu ucgacgagcc caucgggucu      240

guugaugcag gauuuuucgc ccgacagaaa augucaaguc ucagcccuca agacgauaga      300

uuugaggaca cauauaggcu guucaacaau cagaccgaua aggcguuuca cgacgaauac      360

ccaacuacau aucaucuucg gcaggcucug augaacuccg aucgucaguu cgacauucgc      420

gagaucuacu uggccauaca ccauaucgug aaauauagag gcaauuuucu caggucuggc      480

aagccggcag auuucaagac cgggaaacug gaccugaagg aaaaauuuga ggagauuaac      540

gccgauuggc aaacgcucau cucggaagac gcuccccagu uaccuguaga cgaucugacu      600

accauuaagg cagugcuugu cgacggauca cggagccgaa gugaucgcca gagagguuug      660

gugaaacugu ggauggccaa ugagacaguu gaaaagaacu ggaagccagu gaugacugag      720

cuagcuaaag ccauccucgg ccugaagacc aaacuuuacc uguugacagg gcucgaccug      780

cccaaggaag aucagaaagc guggaccuuc uccuuagaca cgcuggagga ucaccaagga      840

gacauagaua gcuuuuucga cgaucaugca cagcagcuca uugacaggcu ugccgagcug      900

cgggcuauga uccaauugac ugaaauuauc ccuucuggcc uaaauuucuc ccagucaaug      960

guccagcgcu augaggccca cagaagucac cuggaaaugu acaagcagua cgcacucgac     1020

caaacagauc caaaaaggac caaggcucug cgugccgcgu auaacgccua caucgaccag     1080

caugagauug cagaugacuu uuauacugcu gugaagcggg uaacaaaaga ugaccagagc     1140

gaaaccgccg aggagauaaa ucgacuuauc ggucuggaac aguucaugcc caagcaaaga     1200

acgaaagaga acgggguuau uccuuaccag gugcaccagc aggaauugga ucaaaucaua     1260

gcucaucaga agaaauauua cccauuucuc ggaaccgaga auccggucau ugaccacagg     1320

gaaucucagc ccuauaugcu ggaugaguua guguccuucc gcgugccuua cuacgucggc     1380

ccaaugguug ccgcaaaccc cgagcugaag acaacugaaa auccuuuugc cuggaugauc     1440

cggaaggcuc agggcgagau caccccaugg aacuucgacg aaaaagugga uagaaagggg     1500

ucagcgacag cauuuauuga gaggaugaaa acuaccgaca cauaucuacu cggagaggau     1560

guacugcccc aacagagccu uuuguaccag cgcuucaugg ugcugaacga acucaauaac     1620

auccgaauug acgacgagcu gcuuaccacu aaucaaaagc agugggucuu ugaucgggug     1680

uucaaaggua agggcacgau aagugccaag cacuugcagg acgcuuugaa acaucacggg     1740

gaaacagaug gacucccgaa gaucucuggc cuggccgacc cuaccaaauu uaacuccgca     1800

uuaacuagcu aucgugauuu ccagaagugg cugcccaaug acauugauag aaaagacagg     1860

accgccgaua ucgagacaau cauuaacugg ucgaccguuu uugaagacac ucauauauuc     1920

guggagaagc uaaaggagau cgaauggcuc acagcugauc aacgccggag aauugccucu     1980

aauagguacc gagguugggg gcgccugucc aaacaccuuu ugacggagau caaggacgaa     2040

cagggccugu caauuaugga ucagcucugg cggacccagc auaacuucau gcaaaucguc     2100

cacgagccag acuaugcaaa acagauagaa gcggcuaaua cugugggaau ggccaagcag     2160

gacaucggca caguaauuaa cgaucuguac accaguccuc agaauaaaaa ggcaaucaga     2220

caaguuuuag ccguggucaa agacauucag aacgcucacc augggguggc ccccagcugg     2280

aucuuuaugg aggcagcuag gggagccgau cagaauccac aacguucccg ccagcggcag     2340

agacagcugc aagaccuuua ugcgaagaca gccgcugauu uggucgacac ugaggugcag     2400

caggaacuca ccgauaagau aaaagacaag gcaaacuuca cggauaggcu gaugcuauac     2460

uuucugcaga auggucaaga cauguacucu aaagagaagc ucaacauuga ucgacugagc     2520

gaauaugaca ucgaccacau ucuuccccag gccuugauca aagaugacuc acuggauaac     2580

cggguuuuag ugcaucagag uauaaaucgc aacaaguccg acaccuucgc uagcgagaag     2640

uacggcgcca aaaugcaggg ggcauggcac caauggagag cggccggacu caucucugcu     2700

aggaagcugc ggaaucuuga aaugcgaccu gaugagauuu caaaauaugc cacaggcuuu     2760

aucagacgcc agcugguaga gacuaggcag gucauuaagu uggugaccga aauccucagu     2820

caccaguaca acccagagga aacaaaacug guuagcguga aggcaggucu aucccaugag     2880

cugcgucggg aggcugggcu cccgaagcug agagaccuua augauuauca ccaugccaua     2940

gacgcauacu uggccgcuag gauuggcacu uaucuguuaa aacgcuaccc ccgacucgaa     3000

agauucuuug ucuacggaca auuccagacc ucucggcugg aucuuaggcg cuuuaacuuc     3060

auccacgacc uggugaaguc gaaacagggc guaaucgugg auagcgacac gggggagauu     3120

cuaugggauc gucaaaagga caucgacuau auuaaacauc ucaugggacu gaagcacuug     3180

cugguuacac acgaagucua cgauuccgcg gccgagaugu augcacagac cauauuuaag     3240

gcuucagaca augauaguaa aguguuaauc ccuacuaaga acgacagacc aacagccauu     3300

uacgguggcu auaccaaacg gaggaauggg uaccucgcca uccugaagau uacugagaaa     3360

aaggcugaga ccuaucgcgu ggucggaguu cccacacuuu acaugucuca gcuggcaacg     3420

gcccgaagcc auuccgugga ggcggaaaaa gcucugcuca agucacugau ggccccugag     3480

auccuaagcg aaucuaaggu gguauccuuc gaggucgugg uuagucacgu gcaucugaac     3540

cagcuuguca gagauaccgu guuuggccca gcacacuacu ucggucucgg gcccuuccgg     3600

uauguuagga auuuucauca acugguguug ccguucaaag accaggaaau acugguaaag     3660

cgcacuccua cagaugagga guuaaacacc gucuuugaag acaucugcug gcaaguggag     3720

aauuacuucg ccguguuuga uauuaacggc uucagaaaug cucucaaaaa gagcaucgac     3780

aaauuugaac agcugccaau ugagaacguc uucaaggaua agaaacuugu uacacaggga     3840

aagaaagaag ugcuggcauu uguaaugcag ggcuugcacg ccaacgcguc uuauaggaau     3900

cucaaggugu cccuggggau caaaacuccc cuaggacagc ugcagcuuac caaguucaug     3960

cucucacggg acgcugagcu gauagaugag aguccuacgg gcuuguuucg ucgcauucga     4020

agauuaaagg accuguga                                                   4038


<210>  3002
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3002
augggcaaca agcugagaaa uguggacgau uacuaucucg ggcuugacau cggaaccgag       60

agcgucgguu gggccguuac agauaaauac uaugugauuc ccaaguucaa aggcaacgcu      120

auguggggag uacaccuguu ugacgaaggg gagacuuccg ugaagaggcg gggcuuccgc      180

ucugcacgac guagaacgca gaggaaacgg gaacgcuugg ccuuacugga ggcgcuauuu      240

gaugaagaga uaugcaagaa ggacuguacc uucuuuagaa ggcuccauga gucagaucug      300

uggccugaag acaaaagugg ugaugacaag uacgcugucu ucgccgauga gaauuauaca      360

gacaaagauu uucacaagga auacccaacu aucuaucauc uucgguacga gcugauuaaa      420

aacaaggagc cgcacgacgu gaaguugguu uuccucgcac ugcaucacau cauuaaaaau      480

cgcgggcacu uucuguucga cagccucucc ucugccgaag auuuuaacuc ggcuuacgag      540

accaugugcu cauauuuaag cgacaaugaa auccaacuga acuguaguga uauagagcag      600

uucaaggcaa uccuuaaaga ccgaagacag ggaaagauug aaaagacaaa gaauuuggcc      660

ucccuguuug gcaucaccaa aaagucugau cccgugagcu acgcugucau ggccgugcua      720

uccggcucaa cuguaaagau gugcgaccuc uucggggaug acgagcugaa aacaguugag      780

aagaacaaua uuaguuucgc gagcggauau gaagagaacg aagagaucua cgcaucugug      840

cuuggugaua gguuugaacu ggucaaaaua uugaaggccg uguaugacug ggcugugcuc      900

gccgauauuc uggacggcaa aaaguacauc uccgaggcaa aggucgcugu uuaugagaaa      960

cauaagaaug acuuaaaaac ccugaagaaa uacgugaaga cguaccuccc ugaaaaguau     1020

aacgagauuu ucaaaaucuc agaugggaag aaagacaauu acguagccua uagcggacac     1080

aucaaggaaa guggcaaaac uggugagcuu aagaagaccu guaacucuga aaacuuuugc     1140

gcguaccuga aaaagacauu ggggaaaaau gccgaucuau ccuauaagga gauguucgac     1200

gagauugaaa acaaaacuuu uaugccaaaa caggugacca aaagcaaugg agucgugccc     1260

augcaaguua acgaggaaga acugaaggca auacucgaua augcuaagga cuaccugucg     1320

uuccuuaacu cuugugauga gaauggcgug uccgucuaug acaaaaucau uaagaucuuu     1380

gaauaccgga uaccuuacua ugugggccca cugaacacac auaccgaaaa uucaugguug     1440

aaacguaagg augggguaaa aauuuacccc uggaacuucg acgauguugu ggacaucgau     1500

aguagcgccg aaaaguuuau caauaaccuc acguccaagu gcacuuaucu gccugagugu     1560

gacgucauuc caaaaugcuc uuuacuguac agcaaguuca cagugcuaaa ugaaaucaac     1620

aaucucaaac ugaacggaga ggacauuccg gucgagcuua agcaggguau auauaaagau     1680

uuguuuauga cccgcagaaa ggugacuguu aagcagauca aagacuaccu gaaggcuagg     1740

ggcuguuaug auaacaauga cgugcucuca gggauugaua caaccguaaa aaguucccuu     1800

aagagcuacu ucgacuuuaa aggauucgaa ucucugacgu ucgaugacaa ggagucaauc     1860

aucgccagua gcgucauuuu uggcgaugac aagaaacugu ugaagaaacg gcucaagaaa     1920

cuguuccccg aaaaguuaac cgaugaggac auaaagaaga ucugcaagcu gaaauacaca     1980

gguugguccc gacuaucuaa ggaauuucuc ugcgaugugg aguucacuua uaaaaagacc     2040

ggggagguua ugaacgugau uacagcacug ugggaaacua auuacaaccu uaugcagauc     2100

uuguauucgg aggacuuuug uugcgacggc gaugagaaaa agaaucugaa cgacaagcuc     2160

gaugagcuga gcuacaccgg agaccaacac uccuuacgca aagccgucga ugcucuguau     2220

gugucaccua agguaaaaag accaauucuu agggcguugc ucaucgcaaa ggaaauagag     2280

aaaauccagg agcggccccc uaagaagauu uucguggaag uugcccgcgg caucgacaau     2340

agagaggaaa ggcaccguga uagucggaaa aaccgacuga uggagcuaua caagaauugu     2400

aaaaaggagg cugaaaaauu uugcccauau uucgacgccc ugucuaagaa gcucagcgau     2460

gaggacgaau ccaaacugag aagggauaaa cuuuacuuuu acuauauuca guuggggaaa     2520

uguauguaca caggagagga aaucucacug ggugaguuaa ugaacggcaa ugggcgcuuc     2580

gacauagacc auauuuaucc ccggagcaag aucaaagaug acucucucac caacaagguc     2640

cuggugaaua agacugugaa caauaacaaa acgaacgucu acccgauuga ugcagccauc     2700

caguccaaga ugaguccuua uuggcaaaug cuuagaagcu cuggacugau auccgaggaa     2760

acauacaaaa gguugacccg caagacuccc cucaccgacg augagcuguc agcuuuuguu     2820

agucgacagc uaacagaaac ccagcaaagc acuaaagccg uggcacacau ccuggagcag     2880

cucuauccaa agccuaaaac agaaauugug uacguaaagg cggcucuggu ccaugccuuc     2940

cggcagucga augacaugcu uaagugccgu gaggugaacg auuugcacca ugcaaaagac     3000

gccuaccuga auaucguugu gggcaacgcu uauaauguca aggugacgca caacaaaguu     3060

aauuuuauua aggguuuaca gaacaaugau aaaggguaca ccgugaaccc cgagaaguuc     3120

uucucccacg acaucccagg cgccuggucu guaaaggaua gcaugaauac ugucagaagg     3180

uuuaugaaca aaaauaacau acgcuauaca cgguucccca gaaagaaaca aggaggccuc     3240

uuuaagauua auccucugaa aaaggggaac ggacaggugc cacuuaaggc aucaggugcu     3300

aggaguugua ucgaaaaaua cggcggguau gacaaggcca ccuccgcgua cuucucuuau     3360

guggaguaca cagauaaaaa gggaaaagcc guccgacaga uguuuccggu uaaccuguac     3420

gaagugaagg aguaugaagc ugacaugcag ggcuucguaa cuagccgcau cgcagaggcc     3480

uccaauguga agauucuaau ccccugcguc aaauacgacg cuuguauuuc auuugauggu     3540

ugccggaugu ucauaaccaa gaaaacgggg aacagaaucg gcuauaagcc uggaaugcaa     3600

cucgugcugg gcuacaaaua ugagaaguac auuaggaaua ucgaaaacua uuugaccaaa     3660

cauaaggacc gugagauuau cccacgcgau aauauaagcg ccgaagagaa caucgagcug     3720

uacgacauuu uaacagggaa gaugcucaau acuguucuga gugugaaauu uucuggaauc     3780

ggcaagaaac uugaaaagcg gagagagaaa uucguccugu guucccccga ggagcagugc     3840

ugcguguuga acgaaauucu caagauccug cacgcaaaug uucuaagcgg ugaucugucg     3900

cuuauagggg agucaccuaa caccggaaca augaagacua augcgaaauu uucugagauu     3960

aaggacgugc auaguaucaa acucaucaac caguccguca ccggccuguu cgaacaggag     4020

auugauuugc ugaaguga                                                   4038


<210>  3003
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3003
augggcaagc ccuacagcau cgggcuggac auuggaacca acuccguggg uugggccguc       60

guuacagaug acuauaaagu gccugcuaag aaaaugaagg uacucggcaa uacugauaaa      120

cagucuauaa agaagaaccu ucugggggca uuguuauucg acucaggaga gacggccgaa      180

gcgaccagac ugaaaaggac agcucggcgc cgauacacuc guagaaagaa uaggcuacgg      240

uaucuccaag agaucuuuac cggcgaaaug aacaaagugg augagaauuu cuuucagcgc      300

cuggacgaua guuuccuugu cgacgaagau aagagaggug agcaccaucc aauuuuuggg      360

aacaucgccg cagaggugaa auaccacgac gauuucccga caauuuauca ucugaggcgg      420

cacuuggccg acaccagcaa gaaggcugau cuccgccugg uuuaccuggc acucgcccau      480

augaucaaau uucgaggaca cuucuuauau gaaggcgacc ugaaggcuga gaauacugac      540

gugcaggccc uuuuuaaaga uuucgucgaa gaguacgaca agacaauaga ggaaucccac      600

uugucugaga ucaccgugga ugcgcugucg auucuaacgg aaaaaguauc aaagagcagu      660

agacucgaga accugaucgc acauuacccc acugaaaaga aaaauacccu uuuuggcaac      720

cugauugccu ugucccucga ccugcacccu aauuucaaga caaacuuuca guuaucugag      780

gaugcuaaac ugcaauucag caaggacacu uaugaggaag aucucgaggg guuccuugga      840

gaaguuggug acgaguacgc cgaucuguuu gcauccgcua aaaauuugua ugacgccauc      900

cuacugucag gcauacucac cguggaugac aacaguacaa aggcgccacu gagcgccucu      960

auggucaaaa gguacgaaga acaucagaag gaccuuaaga aacugaagga uuucauuaaa     1020

gugaaugcac ccgaccagua uaacgcuauc uuuaaggaua aaaacaagaa gggguacgcc     1080

uccuauauug agucaggagu gaaacaggac gaauuuuaca aguacuugaa aggcauccuc     1140

cugaagauca augguagcgg ggauuucuua gacaaaauug aucgggagga cuuccugcgu     1200

aagcaacgca ccuuugauaa cggaaguaua ccucaccaga uccaccuaca ggaaaugcau     1260

gcuauucuca gaaggcaggg cgagcacuau ccauuccuga aggaaaauca agacaaaauc     1320

gagaagauac uuacguuucg gauucccuac uaugucggcc cuuuggcccg aaaagggucu     1380

cgcuucgcau gggccgagua caaggcugau gaaaagauca cuccauggaa cuuugacgau     1440

auccuggaca aagagaaauc cgcggaaaag uucauuacaa gaaugacccu caaugaccug     1500

uaucuuccgg aggagaaggu ucugcccaaa cauagcccuu uguacgaagc auuuacugug     1560

uauaacgagc ucacaaaggu aaaauacgug aaugaacagg gagaggccaa guucuucgau     1620

accaacauga aacaggaaau cuuugaccac gucuucaagg agaauaggaa ggugacgaaa     1680

gauaagcugu uaaacuaccu gaauaaagag uuugaagagu uccggauugu uaaccuaacc     1740

ggucucgaca aggaaaauaa ggcuuuuaac ucgucucugg gcacauauca ugaucuucgc     1800

aaaauauugg acaaguccuu ccuggaugac aaagccaaug agaagacuau cgaagauauu     1860

auccaaaccc ucacacuguu ugaggacaga gagaugauca ggcagcguuu acagaaauac     1920

ucagauauuu ucacuaaggc acagcugaaa aaguuggaac ggcgacacua uaccgggugg     1980

ggaagacuua guuacaagcu cauaaacggc aucaggaaca aagagaauaa gaaaacaauu     2040

cuggacuauc uaaucgauga cgguuacgcc aaccgcaauu uuaugcaacu gauuaacgac     2100

gaugcucuca gcuucaagga agagaucgcc cgggcacaga uaaucgacga uguggacgau     2160

auugcgaaug ucgugcacga ccugccaggg ucccccgcua ucaaaaaggg cauucuucag     2220

ucuguaaaaa ucguugauga auuggugaag gucaugggac auaacccugc caauauaauu     2280

aucgagaugg caagagagaa ccagaccacu gacaagggca ggcgcaauag ccaacagcga     2340

cugaaauuac uccaggauuc acugaagaac cuugacaauc cagugaacau uaaaaauguc     2400

gaaaaccagc aacugcagaa ugaucgguug uuucucuauu acauccagaa cgggaaggac     2460

auguacacgg gagagacacu ggacauaaac aaucuaaguc aguaugauau cgaccacauu     2520

aucccccaag ccuucauuaa agauaacucc cuggacaauc gugugcucac cagaagcgau     2580

aagaacaggg guaagucuga cgauguuccg ucaaucgaag ugguacauga gaugaaaagu     2640

uuuuggagca agcugcuuuc cgucaaauug auaacucagc gcaaguucga caaucugacc     2700

aaagcugagc ggggcggguu aacagaagag gauaaggccg gauucauuaa gagacagcuc     2760

guggaaacca ggcaaaucac uaaacacguu gcacagaucc uggacgagcg auuuaacaca     2820

gaguucgaug gcaauaagcg ccggauuaga aacgugaaaa ucauuacgcu uaagucuaau     2880

cuggucucga acuuuaggaa agaguucgag cuauacaagg ugcgugaaau aaaugacuau     2940

caccaugcuc acgaugccua ccucaacgcg guagugggua augcccuguu gcugaaguau     3000

ccucaguuag agcccgaauu uguuuacggg gaguauccaa aauacaacag cuaccgcucc     3060

cggaagucag cuaccgaaaa auuccucuuu uauaguaaua uccugagauu cuuuaagaaa     3120

gaggacauuc agacuaacga ggacggcgaa aucgcaugga acaaggagaa gcauauuaaa     3180

auccuuagga agguccuguc uuacccucaa gugaauauag ugaaaaagac agaggaacag     3240

accggaggcu ucagcaaaga auccaucuug cccaaggggg agucagauaa acucauucca     3300

cgaaagacaa agaacagcua uugggacccc aaaaaguacg gagguuuuga uucuccuguc     3360

guugccuauu ccauccuggu guucgcugac guggagaaag gcaagaguaa aaagcuacgg     3420

aagguacagg auauggucgg gauuacuauc auggaaaaga aacgcuucga gaaaaaucca     3480

guggacuuuc uggaacagag aggauacagg aacguucggc uugagaagau aauuaaacuc     3540

ccgaaguaua gccuguucga guuggaaaau aagcgaagac gccuguuagc cucugcaaaa     3600

gagcuccaaa agggcaacga acuggugauc ccccagaggu uuaccacgcu ucuguaccac     3660

uccuaccgua ucgagaaaga uuaugaaccu gagcaucggg aguacgucga aaagcacaaa     3720

gacgaguuca aggaauugcu cgaguauauu ucaguguuua guagaaagua cguucuggcg     3780

gauaauaacc uaaccaaaau agaaaugcug uucagcaaga auaaagacgc cgaggugucg     3840

ucccuugcua agucuuuuau cagccuccug acauucacug ccuuuggugc accagcugcc     3900

uucaacuuuu ucggggagaa uauugauagg aaacgcuaua ccucaguaac agaaugcuug     3960

aacgcaacuu uaauccacca gaguauuacc ggccuguacg agacgcgaau cgaccucucc     4020

aagcugggag aagauuga                                                   4038


<210>  3004
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3004
auggaccaga aguacagcau cggccuggau auugggacca acuccguggg augggccguc       60

guuacagacg auuauaaagu gcccgcuaag aaaaugaagg uacucgguaa uacugacaaa      120

caaucuauaa agaagaaccu ucugggcgca uuguuauucg auucagggga gacggccgaa      180

gcgaccagac ugaaaaggac agcucggcgc cgauacacuc guagaaggaa ucggcuacgc      240

uaucuccagg agaucuuugc cgaagagaug aacaaggugg acgaaaauuu cuuucagaga      300

cuggaugaca guuuccuugu cgaugaggac aaaaggggag agcggcaccc uauuuuuggc      360

aacaucgcag ccgaagugaa guaccaugau gacuucccaa ccauuuauca ccuucgcaaa      420

cauuuggcug auaucagcca gaaggcagac cuccgacugg uuuaccuggc ccucgcucac      480

augauaaagu uuagagguca uuucuuaauc gaggggcaac ugaaagccga aaauacaaac      540

gugcaggcgc uuuuuaagga cuucgucgag guguaugaua aaaccguaga ggaaucccac      600

uugucugaga ugacuguuga cgcacugucg auucuaacag aaaagguguc aaaaagcagg      660

cggcucgaga aucugaucgc ccacuacccg gcugaaaaga aaaacacccu uuuuggaaau      720

cugauugccu ugagucucgg ccugcagccc aacuucaaga cgaauuuuca guuauccgag      780

gaugcaaagc ugcaauucuc uaaagacacu uacgaggaag aucucgaggg ccuucugggg      840

gaaaucggag acgaguaugc ugauuuguuc gccagcgcga agaaccuaua cgacgccaua      900

cugcucuccg guauucugac cgucgaugac aauucaacaa aagcaccucu uagugcuagc      960

auggugaagc guuaugaaga acaucagaaa gaucugaaga aguugaaaga cuuuaucaag     1020

gugaacgccc cagaccagua caacgcuauu uucaaagaua agaauaaaaa gggcuaugcc     1080

ggguacaucg agaacggagu caagcaggac gaauuuuaua aauaccucaa gggcauccug     1140

uuacaaauua augguucugg ggauuuccug gacaaaauag aucgcgagga cuuucuaaga     1200

aagcagagga cuuucgauaa cggauccauc ccccaccaga uucaucucca agaaaugcac     1260

gcaauccugc ggcgacagga ggaacacuac ccuuuucuua aagagaauca ggacaagaua     1320

gagaagauuu ugaccuuccg caucccauau uacguuggcc cccuggccag aaaaggcuca     1380

agguuugcuu gggcggaaua uaaggcagau gagaaaauca caccuuggaa cuucgacgau     1440

auucucgaca aggagaaaag cgccgagaag uuuaucaccc ggaugacgcu gaaugaucuu     1500

uaccugccag aggaaaaagu guugccgaag cauagucucc uguaugagac uuucacagua     1560

uacaacgaau uaaccaaggu gaaauauguc aaugagcagg gggaagcuaa guuuuucgac     1620

gccaacauga aacaagagau uuucgaccac guguuuaagg agaaucgcaa aguuacuaag     1680

gauaagcugc uaaacuaccu caauaaagag uucgaagaau uuagaauagu gaaccugaca     1740

ggacuugaca aggagaauaa agcauucaac ucuuccuugg guaccuacca ugaucugagg     1800

aagauccucg acaagagcuu ucuggaugac aaagccaacg aaaagacgau ugaggauauc     1860

auccagaccu uaacacuguu cgaggaccgu gaaaugauuc ggcagcgacu ucagaaauau     1920

ucggauauau uuacuaaggc ucaauugaaa aagcucgaga gaaggcacua caccggcugg     1980

gggcgccugu cuuauaaacu aaucaauggc auucggaaca aggaaaauaa gaaaacaauc     2040

cuggacuacc ucauugauga cggauaugcc aacagaaauu ucaugcagcu gaucaacgau     2100

gacgcacuuu ccuuuaagga ggaaauagcg agggcucaga ucauugguga cgucgaugac     2160

aucgccaaug ugguacauga uuugcccggg agcccugcaa uuaaaaaggg cauccugcag     2220

aguguuaaaa uaguggacga guuagucaag gugaugggac acaacccagc caauauuauc     2280

auugagaugg cucgcgaaaa ccaaaugacu gauaaaggcc gacggaauag ccagcagcgu     2340

cucaagcugc uucaggacuc ccugaagaac uuggauaauc ccgucaacau caaaaaugug     2400

gagaaccaac agcuccagaa cgacagacug uuccuauacu auauacaaaa ugggaaggau     2460

auguacaccg gagaaacacu ggacaucaac aaucucucuc aguacgauau ugaccacauc     2520

auuccucagg ccuuuaucaa agacaacagc cuggauaaua ggguucuuac ccgcucagac     2580

aagaaccggg guaaaaguga ugacgugcca uccauagagg uaguccauga gaugaaguca     2640

uucuggucua aguugcuguc agugaaauua auuacucaga gaaaguuuga uaaucucacg     2700

aaagcagaaa ggggcgggcu gacagaggaa gacaaggcug gauucaucaa acgacaacuu     2760

guugagaccc gccagaucac uaagcacgug gcccagauuc uggaugaacg guucaacacc     2820

gaguuugacg gcaauaagag aaggauccgu aacgucaaaa uuauaacacu aaagaguaau     2880

cucgugagca acuuccgcaa agaguuugaa cuguauaagg uacgggagau caaugauuac     2940

caucacgcgc acgacgccua uuugaacgcu gugguuggua augcacuguu acucaaauac     3000

ccccagcugg aaccggaguu cgucuauggg gaauacccua aguauaacuc cuacagaucu     3060

aggaagucgg ccaccgagaa auuucuuuuc uacagcaaca uucugcgauu uuucaagaaa     3120

gaggauaucc aaacuaauga agacggcgag auugcuugga acaaggaaaa acauaucaag     3180

auauugcgga aggugcucuc cuauccccag gugaauaucg ucaaaaagac agaggaacag     3240

acgggaggcu uuucaaaaga gaguauucug ccaaaggggg agucugauaa acuaaucccu     3300

cgcaagacca aaaacagcua cuggaauccc aagaaguaug gagguuucga cuccccaguu     3360

guggccuacu caauucuggu guuugcagac guagaaaaag gcaagagcaa aaagcugaga     3420

aaaguccagg auaugguggg gaucacuaua auggagaaga agagguucga aaaacacccc     3480

guugacuucc ucgagcaacg gggauaucga aacgugagac uggagaagau uaucaaauug     3540

ccuaaguacu cucuguuuga auuagagaau aaacgcaggc gucuccuggc guccgcccgg     3600

gaacuucaga agggcaacga gcuggucauc ccacagagau ucacaaccuu gcucuaucau     3660

aguuaccaaa uugaaaagaa uuacgagccg gagcacaggg aauaugugga gaaacauaag     3720

gaugaauuua aagagcugcu agaauacaua agcguuuucu cucgcaagua ugugcuggcu     3780

gacaacaauc uuacaaaaau cgagaugcuc uuuuccaaga acaaggaugc cgagguauca     3840

agucuggcaa agagcuucau uucguuguua acuuuuaccg cuuucggugc ccccgcagcc     3900

uuuaauuucu uuggggaaaa caucgaccga aaaagauaca cguccgucac cgagugccug     3960

aaugcuacac ucauucacca gucuaucacu ggccuguaug aaacccggau agaucuuagc     4020

aaacugggag aggacuga                                                   4038


<210>  3005
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3005
augaagaaag aguacagcau cggccuggac auugggacca acuccguggg augggccguc       60

auaacagaug acuauaaggu ucccgcuaaa aagaugaaag ugcucgguaa uacugauaag      120

cagucuauca agaaaaaccu ucugggcgca uuguuauucg acucagggga aacggccgag      180

gcgaccagac ugaagaggac agcucggcgc cgauacacuc guagaaagaa uaggcuacgg      240

uaucuccaag aaauuuuuac cgaggaaaug aacaagguag augagaauuu cuuucagcgc      300

cuggacgaua guuuccuugu ggaggaagac aaacagggaa gcaaguaccc uaucuuuggc      360

acacugaagg aggaaaaaga guaucacaag aaauucaaga ccauuuacca uuugagagag      420

gaacucgcca acuccaaaga gaaggcagau cugaggcugg ucuaucucgc cuuagcucac      480

augaucaaau uucgggguca uuuccuguac gaaggggacc uuaaggcaga gaauacugau      540

gugcaggccu uguuuaagga cuucguugaa gaguacgaua aaacaauaga ggaaucucac      600

cugucggaga ucaccgugga cgcucuauca auucucacgg aaaaggucag caaaaguucc      660

cgccuggaga accuuaucgc ccauuaucca acugaaaaga aaaauacccu guuuggaaac      720

uugauugcgc ucucucuggg cuuacaaccg aauuucaaga caaacuuuca gcugagcgag      780

gacgcaaagc uccaguucuc caaagauacu uacgaggaag accuugagga acuguugggc      840

gagaucgggg augaauaugc cgaccuauuc gcuucagcca agaaucugua cgaugcaaua      900

cuccugagug guauucuugc uguggacgau aacaccacaa aagcgccccu gagcgccucu      960

augguaaagc gauaugagga gcaccagaaa gacuugaaga agcucaaaga uuuuaucaag     1020

guuaaugccc cugaccaaua caacgcaauu uucaaagaua agaacaaaaa gggauaugcu     1080

ggcuacaucg aauccggggu ggagcaggac gaauuuuaca aguaucugaa aggaaucuua     1140

cugaagauua auggcucagg ugacuuccua gauaaaauag acugcgagga uuuucucaga     1200

aagcagagga ccuucgacaa cgggagcauc ccacaccaga uucaucugca agagaugcac     1260

gccauccuuc ggcgucaggg agaacauuac cccuuuuuga aagagaauca ggauaagaua     1320

gagaaaauuc ugacguuccg caucccuuau uacgucggcc cacucgcuag aaagggcagu     1380

agguuugccu gggcagaaua uaaggccgac gagaaaauca cucccuggaa cuucgaugac     1440

auucuggaua aggaaaaauc ugcugagaag uuuaucacac ggaugacccu uaaugaccug     1500

uacuugccug aagagaaagu gcucccaaag cacuccccgc uguaugaaac uuucacagug     1560

uacaacgagu uaaccaaggu caaauacguu aaugaacaag gggaggcgaa guuuuucgau     1620

acgaacauga aacaggagau uuucgaccac guguuuaagg aaaaucgaaa aguaaccaag     1680

gauaagcugc uaaacuaucu caauaaagag uucgaagagu uucgcauagu gaaccugaca     1740

ggacuugaca aggaaaauaa agcauucaac agcucguugg guacuuacca ugaccugaga     1800

aagauccucg auaaaucuuu ucuggacgau aaggccaaug agaagaccau ugaggacauc     1860

auccagacau uaacucuguu cgaagauagg gagaugauuc ggcagcgccu ucaaaaauau     1920

uccgacauau uuaccaaggc ucaguugaaa aagcucgaaa gaaggcacua cacaggcugg     1980

gggcgucugu cauauaaacu aaucaacggc auucggaaca aggagaauaa aaagaccauc     2040

cuggauuacc ucauugacga uggauaugcc aaccgaaauu ucaugcagcu gaucaacgac     2100

gaugcacuua guuuuaagga ggaaauagcc agagcucaga ucauugguga cgucgaugac     2160

aucgccaaug ugguucauga cuugcccggg agcccugcaa uuaaaaaggg cauccugcaa     2220

uccgugaaaa uagucgauga guuagugaag guaaugggac acaacccagc gaauauuauc     2280

auugaaaugg cuagggagaa ccagacuacg gacaaaggcc gccguaauuc ucagcagaga     2340

cucaagcugc uucaagauag ccugaagaac uuggacaauc ccguuaacau caaaaaugug     2400

gaaaaccagc agcuccagaa ugauaggcug uuccuauacu acauacaaaa cgggaaggac     2460

auguauacag gagagacccu ggauaucaac aaucucucac aguacgacau ugaucauauc     2520

auuccucagg ccuuuaucaa agacaacagu cuggauaauc gcguccuuac ucgauccgac     2580

aagaaccggg guaaaagcga cgaugugcca ucuauagagg ucgugcacga aaugaaguca     2640

uucuggagua aguugcugag cguuaaauua auuacccaac guaaguuuga caaucucaca     2700

aaagcagaga gaggcgggcu gaccgaagag gauaaggccg gauucaucaa aaggcagcuu     2760

guggaaacuc gccagaucac aaagcacgua gcucagauuc uggacgagcg guucaacacg     2820

gaguuugaug gcgcccaaag aaggauccga aaugucaaga uuauaacccu aaaauccaac     2880

cucgugucua auuuccgcaa ggaauuugag cuguauaaag uucgggaaau caacgacuac     2940

caucacgcac augaugcuua uuugaaugcc guggucggua acgcgcuguu acucaaguac     3000

ccccagcugg agccggaguu cguguauggg gaauacccua aauacaauuc guauagaagc     3060

aggaaguccg ccacugagaa guuucuuuuc uacucaaaca uucugcgguu uuucaaaaag     3120

gaagacaucc agacaaauga ggauggcgaa auugcuugga acaaagagaa gcacaucaaa     3180

auauugcgca agguacucag uuauccccag gugaacaucg uuaaaaagac cgaggaacaa     3240

acaggaggcu uuucuaagga gagcauucug ccaaaagggg aauccgacaa gcuaaucccu     3300

cggaaaacua aaaauucaua cuggaacccc aaaaaguaug gagguuucga uagcccaguc     3360

guggcauacu cuauucuggu guuugccgac gucgagaagg gcaaauccaa gaaacuuaga     3420

aagguucagg auaugguggg gaucaccaua auggagaaaa agagguucga aaagaauccc     3480

guggacuucc ucgagcagcg aggauaucgg aacguacgcc uggagaaaau uaucaaguug     3540

ccuaaauaca gucuguuuga auuagaaaau aagagaaggc ggcuccuggc uagcgcccga     3600

gagcuucaga aaggcaacga acuggucauc ccacaaagau ucacgaccuu gcucuaccac     3660

ucuuaucaga uugagaagaa uuacgagccg gaacaucgcg aguaugugga aaagcacaaa     3720

gacgaguuua aggaacugcu agaguacaua uccguuuucu caaggaaaua ugugcuggca     3780

gauaacaauc uuacaaagau cgagaugcuc uuuaguaaaa acaaggacgc ggaagucagc     3840

ucgcuggcca aauccuucau uucuuuguua acuuuuaccg cuuucggugc ccccgcagcu     3900

uuuaauuucu uuggggagaa caucgaucgu aagcgguaca caagcgugac ugaaugucug     3960

aaugccaccc ucauucauca gucaaucacg ggccuguaug agacaagaau agaccuuagu     4020

aagcugggag aggauuga                                                   4038


<210>  3006
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3006
augaaccaga ccaagaauua cgacaucggc cuggauauug gggugggaag cgucgguugg       60

acaauaacug acucccaauc ucagaaaguu cucaagguga aaggcaagca ggggaucgga      120

guacaccuuu ucaacgaggg ccagacggcc gaagagagaa ggcaauuucg gaccaaucgc      180

cgacgucuga aaagaaggaa guggcgguug cgcuuacuga gagaacuauu cgaugagccc      240

auuucaagua ucgacccuga auuuuucgcu aggcucaagu auuuuaacga gagcuccaaa      300

gauccaaaga auaaagguua ccagccgcag cugcaugacg aucuuaagga cguggauuuc      360

uaucggcagu accccacaau uuaucaucug cgccacgcau ugaugacuca aaaagagaag      420

uuugacaucc gagauauaua ccucgccauc caccauauug ucaaguauag agggcacuuc      480

cugaugaacg gacacucuuc gucauuuagc guggcgcaga ccgacuggaa agcugaucug      540

acaaaacucu ucgaauuaau ccaggagacc aggacuaauc uggacuuuag uuucgaccuu      600

acagaaauug auaccaucaa auccuugcug uuugacaaga accuaucucg gaaugaucgu      660

cagaaagaga uaguuggccu cauuaacaau aacagccaac uggacaagaa agauaaggcc      720

cuuacgaagc agcuggugga guccgucgug aaaggcaucg uagggaugaa gacucgcacc      780

gacauuuugc ucggauacga aguugauaau aaagagcagu caaaggcaug gaacuucagu      840

aucgacgaac uggaugacca aauggccaca aucgauccug cuuuaagcca aaauucucag      900

gcacaugaca uuauaacucu gauccagaaa cucuacauga ccgugcagcu ugcccagauu      960

gucccagagg gucaguccgu gucagaaaug augaucaaca gauauaaccu guacgcugag     1020

caguugcaac agcuaaagga cuauaagaaa auaaaucugg agacaggccu ccugcuuagg     1080

gaagccuacg augagcugau gaccaaggcg uugacggcag cccccgggaa aacuauucac     1140

gacacauucu auaacaaggu gaaaaagauc cucaaggauc aggacaauaa acaggcucug     1200

agcaucaagc aagccauuga agauaguaac uacuuaaucc cucugcggau uaaaucuaau     1260

ggagucauac cauaucagcu acaucagcga gagcucgacg caaucauuga aaaccagaag     1320

gaguuuuacc ccuuccuggc uaccccuaau ccaguugaug gccaccaaga ggcccuuccg     1380

uacaaauugg acgaacugau cgguuuuaag gugcccuauu acguagggcc uaugguggau     1440

ccagaggucg acuccgauca gaacuucgcg uggauggugc gcaaggaaaa aggagagauc     1500

acucccugga auuuucacaa caagguugac auuacacaga gcgccgauaa uuucauaaaa     1560

agaaugaaga ccacggacac cuaucucauc ggcaaagacg ugcugccuca aaacucgcuu     1620

cuguaccaga gguauacagu cuugaaugaa cucaacggca uuaagguggc aaauaaaaag     1680

cugacuaucg agcagaagca gcgguuauac gaucaacugu uuaaaaagaa aaagaccgua     1740

aagaagcagg acauucuaaa caaccucguu gcugccgggg agauccauga agaugacaau     1800

cugacaggac uuucuaacgg uacuaccuuc aauaacaauu ugggcuccua uauagauuac     1860

ugcaagauca uugggcagga caaacuggag gaugcuucaa agcaggccga caucgaacgc     1920

auuaucgagu ggaguacaac cuuugaggau gcaaacauau uccucacuaa acuggccgaa     1980

auuaauuggu uaacggagga ccaaagacag aagcugagcc agaucaggua ccguggaugg     2040

ggccaguuuu ccaaaacacu uuugaccgau auuaaggaca acaaggauaa uucuaucaua     2100

gacaaccuca ugagcacuaa aaagguguuc augcaaauca uuaaugacac cacaaucaaa     2160

cagaagauug augacuauaa ccaguaccac cugaaucaug cugaucuaaa agacaccauc     2220

gcgaaccugu uuacuucacc acaaaauaag cgggcaauaa accagacacu ccuggucauu     2280

gaagauauca ucaacgccgu gggucacaag cccaaucgaa uuuucaucga gagugcuaaa     2340

gacaacagaa augccaagaa ccagcuuacg caguccaggu ugaaacgcau ugucaagcaa     2400

uucgaagaua auaaacagca uguggaccag agcguuuaug aggagcugaa gcaguuagau     2460

gacgggucuc acgcaucaau agaugaccgg cuccugcuuu acuuucugca aaacggcaaa     2520

gacauguaua ccggacagcc gaucgauauu agucaguuga gcacuuccca guaccacauc     2580

gaccauauuc ucccucaagc caaggugaug gauaaugcuc uggacaacag aguacuuguc     2640

ucuucgaagg ccaauacaga aaagagcgau agguuccugu ccgagauccc ccaguuucag     2700

cagcgcucac ucugggcaga acuggagcga augggcuucc uuaccaaggc gaaaaaggaa     2760

cgguugcuga ugacaaguga cgaguuuaaa caaaacucug ugcguuucau agcuagacag     2820

uuaguugaga cuaggcagau cauuaagcuc gugaaucaac ugaucauuca ggaacaccca     2880

gaggucaagc uagugaacau caaagccgau cugaccaaug cauuucgcaa gaaauucaac     2940

uuuguaaaga aucggaacgc caacgacuau caucacgcuu ucgaugccua ccuaacgagc     3000

cucauagggu cccaccuguu gaaaucacau ccuaaguggg aaccccuguu uguguacagc     3060

gacuaugaua agauuaaauu cgacaaugca ccacagggag uuaacaucau gaaggaguuu     3120

gaacagucua aauuagcuga ugagaauggu gagaucauuu ggaacaagga cgaaaugaug     3180

gccuaccucg agagacugua uaauuccagg caaauacuua ucaccaaaaa ggucuacgcg     3240

cgacacggca accuguucaa gcagacaauu uucaaagccg gggaaaguga caauuugauc     3300

cccgugaaga acaaucgcag cgauauugcu aucuauggag gcuacaaauc uaaacaggug     3360

uccuauuacg cacucauauc acugcauaac aaaaagcggc agaagaggcu agucgccguu     3420

uaccugaucg acauucaaaa acuucagaau cucaacgaga gugauuauuu uaagacucug     3480

cacaccauca uuaaagagag acauaauauc aagccugagu ucgacauauu ggcugauaaa     3540

auuuaccaga accagcugau caucgacaau aagaacggua gcaaucgucc auuucgcauu     3600

gaaggggauu ucaaaaacga ccggcaauua uaucucccgc ugcaguacca gagauggcuu     3660

gccucgaagg aacccacaga ugagaagcug aacaggaucu uugaccacau augugaacaa     3720

uuggcagauu auuucccucu cuuugcgggc aaugccugcg acaaacugcu agauaaccga     3780

gacaaguucu accagcuguc cauuaaaucu gaguuuaaug acaagggaaa acuuaucgcu     3840

gagggcaagc ucagcgugau ugauaagcug uugguggcca ugcacgcaga cucacggaac     3900

aguaauuuag cuguaaucgg gcuguccucu agcuggggac gcuucaaacu ccaugguggc     3960

aacgaagcca uagggacuuc cguccagcug auucaccagu caccaaccgg acuuuuuaag     4020

agaauccaaa gguucaauug a                                               4041


<210>  3007
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3007
augcugagcg ccggcgugau gcucggggac gucuccgcuc uuaagucuuc aagaaacagu       60

cuguuccaga aaagcaagaa auugaucgaa aauuccucuu cggauuuaac caagguugac      120

uacucaaaaa ugagggaaau ucugcuaagc auacggaaca ucgcaauucg cuuuuucaau      180

aacgaggauu uuaaggaaag ugacuauuac aaggaguucc ccacaaucug gcaccuccga      240

aagaaucuga uggaauccga uaagaaagcc gacauucguc uuguguaucu ggcguugcau      300

aacaucguaa aguacagagg aaauuuucuc caagagaaca auaaagaucu gagggcugcc      360

aacgcaaaug ccggugcugc agccauagac cugucuaacg cucucgagga auauuucagc      420

uuacacgagg augaauccgc ccugacuugc gugccuaaug ucgcggaccu ucagaugacg      480

uuggagcagc caggccugcg gaacggggag cgccuagaua gaaucguggc agcccucaau      540

augccggaca gggcucgggc caagcuguuu gcaaaggcuu guauuggaca gaaaguugag      600

uucucaaaac uugcccuggg cauggagaaa caagaaggua ccaacgcgua caucugggau      660

gacgacaagu augaugaguu gguggcccuc ugccccgaag acuuuuuccc uguccuggag      720

acacugaaaa acgcauacag ugcuuacgug cugagcgauc uccuugccgg gaagcugucu      780

auuuccgcuu cauugaucga cagcuaugag cagcaucaga augaucuaaa aauaguaaag      840

gaacugguuc gcgaguaccu cggacagaag gaauauggcc gacuguuuag aggcccaaaa      900

uucgccaacg gggacuacaa uuuuaacaag guggcaaaac ccaguuauac uucuuacauu      960

cuuggagagu cccuguguaa uaagcaaggu accacaaggg aagaguuggu caaagagcuc     1020

aagcgggugu ucgaugacug cagcgccauc caggcugaua agcguuauca gaaaauuaag     1080

cagcgccugg aagacgaucc uaaauucuua aucaagcaga aaaccagagc gaacggcgca     1140

aucccauacc agcugcaccu agaggaaaug caucagauua uagagaagca agggaaguac     1200

uaucccuuuc ucguggaaca gcaggaccug aucgagaaac uugucucguc uaggauuccu     1260

uacuauguug gaccauugaa uggcggucac gacccgaacc accccuaccc uacuaaucca     1320

aucgauccca agcggaaauu cgccuggucc gugcgaaagc cugggaugga gcaugaaaaa     1380

gcauauccau ggaacguaga cgaggugaua gauucagaag agacagccga gcgcuuuauu     1440

agaaggauga ccggaacgug uacuuaccug uauggcgaac ccgucgugcc gcggugcagu     1500

cuccuguacg aggaguucug uguucuuaau gaacugaacg gcgcacgcug gggcgaagga     1560

aauggugaca cccacagauu ugauagcucc gacaggacag agaucuugga gcaacucuuc     1620

aagaagcgua aacgggugac ucauaaggcc aucgcuaccu ggcuggaaau uaaccacggc     1680

gucacaaaug ugaaaaucuc ugggacccaa gccgagaacg cauuugaaag caaguuauca     1740

aguuacaacg auuucugcaa ucugcuagga guagacggcc ucgaggaugu uuguccucug     1800

acgaaagacg auauugaaca gauaauccuu ugggugacug ucuuugagga caagaaauug     1860

cugcuccaga agcugcagcg agcguucggu gauaaguuau ccgagaaaca aauugcuagc     1920

aucaugagaa agcccuaugc cggguggggc aggcugucuu caaaacuuuu gacaggaauc     1980

aagcgcgaaa ccccauuugg ccggauuagu auacucgacg ugaugagaga cggggauccu     2040

aggcccggac accaucugga cgcaaugaac uucauggaga uccuacgcga aaaagauuuu     2100

gguuuccagg accgaauuga ugagaucaau aaggaacgga cuaaggagag cggccugcuc     2160

cuguccgucg aggaccuucc agggucuccc gccuugcgua gaucggugaa ccaggcuaug     2220

cacauucuga aagaaaucgu uagcauagcc ggaaaggagc cuuccaggau cgugauugaa     2280

guaacacgcg auccagacga ggagaaacgg ggccauagaa ccgauacgag guacaagcag     2340

uuaucagaag cacucgcugc caucaaaacc gacauugucc aauucgauag uucucugcuu     2400

gcggagcuga aggaacgaaa ggacagcuug gauuccgagc gccucaugcu guauuuucag     2460

cagugcggua aaucacuaua cagcgggaag ccgcuggaca ucaaucggcu cucugaauau     2520

cagguggacc acauaauucc ccaauguuac aucaaagaug acucccugga uaacaaggcc     2580

cuuguuuucg cuagugagaa ucagauuaaa uuggacagcc uguuacucga uaagucuauc     2640

auaagauccc agaaggggag guggcaagag cugcaucgug caggacuuau cucaaacaaa     2700

aaguuucgca aucugacaug cacugaaauu agugaccgga ugaugaaagg cuucaucaac     2760

agacagcuag uggagaccag ccagauuguc aaguuuguga ggcagaugug cgaacaacga     2820

uaugagucgu ccgaaguaau cucucuccgg gccagccugu cacacaauuu gcgcgauaga     2880

uguggguuca uaaaaugcag ggagcugaac gacuaccacc augcucacga ugccuacuua     2940

gcaugucaga uugcgcgguu uaucggaauc cgauauccua gauggcagga cgguauuagu     3000

cgcgaguuca ucaauaagua cauuaaacag cucgugaaga agggcaaauc cucugggaag     3060

uuuuucgccg gaaaaagcgg cuuuauaauc gauuccuuca ugcaugacgg uuuugacagg     3120

gaaacagggg agauuuuccg ugauggcugg gacgcuacuu ucgaaaucac ccggaugaga     3180

aggguucugg gauauaagga uugcuuuauu acacgcaugc cagaggaaca aaccggcgcc     3240

uacugggagg agacuaucua uucaccccuu gacgcuaaaa acgggaagaa ucugcagaua     3300

ccuuugaagc agagcggaaa agaaggcuac cucgauccaa agaaauaugg ugggacgaac     3360

acagcuuuca acgccuacuu uuucgcauuu gucgccaagg acgcucgagg aagagauaaa     3420

uacuucuuug agggcgugcc cguguaucug gcgaagcagc uaaaugaaca accgcagauc     3480

cuggucgacu ucgccaacag uauugcagcu accaagaaau guucuacugc caagauccuu     3540

cgguccaaag uuccucucag gcagaaguuu cuguuggaug guaccagcuu cuaccugggc     3600

gggcgcacag gaaauaaaaa cgugauuuua ucgggcgugg aguuugccug cgggaucgac     3660

auggcuaaga ugauagauac cgcaauuaag cacccagagg aacucucaca aaaagagcug     3720

aucucuauca uucagaugcu ugccgacacu auagaacgua auagugauag acaggcgucc     3780

cugaucaacu uccggagguu cgacgacaag uugcgcaaua uugaugcuau cgccgcaagc     3840

cgaauuauca aaaaccucuu aaauucuaug aacggaguaa agcagagcug ugaccuaucc     3900

gaucugggcg guucaaaaca aaguggguuu augcagagca auauagccac acuuuucgag     3960

agaaucacgu ggugcuauuc uuccauuacc ggaauguuug aacagcgggu cucauucgag     4020

gaccucaaga acggcaucug a                                               4041


<210>  3008
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3008
augaagaaaa gaaacaggga ggaauacuau gugggccugg acaucgggac cagcuccguc       60

ggaugggccg uugcuacacc cgauuaccgg auuccuaagu uccgcgguca caaaaugugg      120

ggcucucgac ucuuugcaga ggggaagcgu gccgcggaaa gaaggcaguu ccggacuaau      180

cgcagaaggc uugcucggcg ccgagacaga cugcaaauau uggaggaauu auuugccgag      240

gagauggcaa aaguggauaa cacguucuuu augaggcuga aggaaucaaa guucuuucua      300

gaggacaaaa ccguaaagga aaaacauauc cucuucagua cagauaaguu ugacgccgau      360

cgguucuaua aagacaaucc aacuauuuac cacgugcgua agcgccugau ggaggaggaa      420

ccgcaggaua ucagagagcu uuuucuggcu guccaucaca uuuugaagua uaggggacau      480

uuccucuuug caggcagccu ggaacagaac ggugacucca ucgaggaacu ggugcggacc      540

cucuucggga aacagggauu agccauacug gaugacacaa ucgauucuaa ugagaugguu      600

aacauucuua agaauaacga gaaaaccaag ucggaccgca aaaaggcugu gcaagaauug      660

uggaaucccc ugaaagacaa ggucaagaaa gcccaggcgg cagagauggc cggcguggua      720

cuaggccuca acucagauau cagcaagcug uucgacaaua gugauuacaa agaccuuaag      780

auuaacuuuu ccucugggga uuaugacgaa aaacgagaug acuacgcuaa uagcaucgga      840

gaugacauag uucuguugga uacuaugaag gcccucuacg acacacugau uuuaucccag      900

auccugcagg guaagcaauc aauuagugag gcaaaagugg aagacuauaa cacccacaag      960

gagcagcuca gagcucuuaa agccauccug aagccucaca aaagguugua caaugagaug     1020

uuccgggaaa acaacaagaa ggaucuaagc aauuaugucg cguacaucuc uuccuauuca     1080

gagggcaaac gcgugaacag agaaggguuu uacaaguaug ugcaggccgu ccugaaacag     1140

guugacgaua gcgacaguaa gcaagcaauu cucgaggcua uaaggcugga aacguuccuu     1200

ccacuguugc gugugaaaga uaaugccgua aucccccauc agauucacca ggcugagcuc     1260

gaggccaucc uggaaacugc acggaagaag uacaaauuuu uaaacgacgu gucugaggga     1320

gucuccguga aggauaaaau aauucaacug uucaccuuuc gaaucccuua cuauguuggc     1380

ccacuaaauc cccauagcgg ugaaacagug gccgacagaa agcaggggac uggauggguc     1440

gugaggaaac agcgcggcaa gguauacccu uggaacuucg cugauauggu ugacgugaaa     1500

gcgucggcag aggccuuuau caccaaucuc acaaacaagu gcaccuaucu gauuggcgag     1560

gauguccuuc caaagcgguc uuugcuguac uccgaguuca ugcuccugaa ugagcuuaac     1620

augaucaaau augacgggca cgaacugccg guggagauua agagauugua cauagaacag     1680

gucuuuaaac aagagcacaa gaaacagacg aagggaaggg ugcucgauuu ccugaagcgc     1740

aaugguuuaa ucacuggcuc agcuacagcc guuauuaccg ggauggacga caguaucaaa     1800

ggagaucugc agagcuaucg agacauggug cggaucuuug gcacugguuu cgaucauucc     1860

auggcagagc guauuauaag auggguaaca cuauucgggg aagccaagga cauccucgag     1920

aacaaaauua gggaagucua cggcaauucu aucagcgauu cacagaugcg cgcuauuaag     1980

aaacugaagu acgccgacug gggacggcuu agucaagcau uuuucaucaa gauaaucggc     2040

ugugauaaau ccggggugga gagaaacauu auuaccugcu ugagggaaag cucucagaau     2100

cugauggaga uccucucaga ccagggaacg uauaucaagc agauaaaagc guggaaccac     2160

caacauacca caaacaugau ggauacucug gacuaccagu uaguugauga ccuguaugug     2220

gcucccagug ucaagcgaag cauuuggcag ucccuuugua ucguggagga auugguaaaa     2280

auuauggguc aggccccuaa gaagaucuuu guggagguua cccgcacaaa ucaagcagag     2340

aaaacuaaga aagauucucg gaagaaaaga cucgucgaac uguacaccgc cauaggcaag     2400

gaaggggcug ccuggaaaaa ggagaucgag aagcaccagg caggagacuu caggcguaaa     2460

aagcuauauc uguacuauac acagaugggc cgcugcaugu acucgcauga accaauugcu     2520

auggacgccc ucuuuaccag cgaguacgau aucgaccaca uuuaucccca aucccugacu     2580

aaagaugacu caugggauaa ccuuguguug gugaagaguc agcugaauaa agaaaagucu     2640

gaccaguacc cuuuacggcc agcgaucaga gagaagaaca aagccuauug gcaguaccuc     2700

uguagggcug guuucauaag cgagaagaaa uaugaacgac ugaugcggac gacagagcuu     2760

accgcagaag aacuguccgg guuuauugcc cgccaauugg ucgaaacuuc acaggcuacc     2820

aaggccguua ucggccuccu ggagagaauc uacaaacaca cagauauuug cuucgugaag     2880

gcagagaccg ugagccaguu uaggcaucag cacaauuuca ucaagauucg ggaacuaaac     2940

aauuaucauc acgcgaaaga cgccuaccug aacauaguag ucggaaaugu guaccacgag     3000

aaguuuacua aaaacccccg agaguucauc gcuagaucua cagaacgcag gccguauaau     3060

cucaaccgua uguacgauuu ugaccugcuu aagcaaggcc ggguuauuug gcaagugccu     3120

aaauccuugg aaacggucca ggccaugaug aagaguaaug augugagaau caccaagaaa     3180

cuguuaguuc agaaggggga gcucuaugac gcaacuauua caaaagcuca uaagaccaaa     3240

gagggaaugu acuucccccu gaagggugcc gacgcaaggc uucgcgaugu gcaccgauau     3300

ggcggguacg aaaagaucaa aauagccuau uacagcguau ucucuuguug cgagaaggcu     3360

uccgacacaa uccauacucu gauuauccca auuccugucu acgcggccgg acuacuccag     3420

accgaagcag aucugacggc uuaugccugg cagaccaaac agaagacaaa acccaucaag     3480

gacauauacu caaugauuac uccaaucuau aaaaaguugu guaucaacac cguggugaag     3540

auugauggcu uuaaauacuu ccuggguggg agaacagagg acuauauaug cuuauacccc     3600

gccaucccug ucguucucga ugaacaagcu gcagccuauc ugaaggcggu gaguaaagua     3660

ggccgggcug acgaugugga gaauacugcc caggcaauug accuuaucac cgccgaggcu     3720

aacgaagcau uuuacgaugu ccuguugaag aaaauggagg ccaagauuuu cgugaaggcg     3780

uaccaggaaa ggguugagaa acucgcuagc gagacggccc gcgaacguuu uagaaagcug     3840

ucgaucggag cacaguguuc cugccuacug gagauacuua accuccuggc caauaacaaa     3900

acaaccucua auuugaagaa auggcugggc gugacuauga gccggaggaa gcaaccaaug     3960

gacuuaacaa agcucgaaga gugcaucguc uaugaaaccu caauuacugg gcuguacgag     4020

aaagcuguga gucuucugug a                                               4041


<210>  3009
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3009
augguggagc acuacgccau cggcuucgac auugggaccg gaagcacagg uuuugcuggc       60

gucgauccca acgggaagcu gguuagaaug aggggcaaaa auguguaugg aguauaccgg      120

uucaagggug gggaagcagc cgcggaccgc cgaggauuuc guacuacgag aaggcggaua      180

aaacgcagaa aguggaggcu ccgguuccuu aaauccuuuu ucgagccuua uaucgcucca      240

auugauccgg aauuuuuccu gcgccagaag uacucugccg acucacccuu ggauccuaag      300

ggcauccgag cagacaaagg cuuacugagu gauaugagcg accaagaguu ucaugccaag      360

uauccaacca uuuaccaccu aagauaucag cucaugcaug agaaaaggca guucgaugug      420

cgggaaaucu accuggcuau gcaccauaua aucaaguacc gugggcacuu ucuuauugag      480

gacggagcag auaacuucga cgccaaacag aucgauuucu ucgaccaauu ccagcgcaug      540

gcugccguca cagcgggucu gcccgaauug gagacucucg ugguuccuga cgaaacccag      600

uccgaugcau uuaagagagu gcuguucgac gagucucaga caaagucgga uaggcaaaaa      660

guccuggccu cacagcucuu agacauucag gauaaucaga gcaaggcuau caaagacuuu      720

auagccgcac ugaccaaggc ugugguaggc augaaagcca accuugcgac uuugacaagu      780

accgccauug aguccacgga uaagaaugac uggacuaaac cacuggggca acuagaugac      840

uuccuccuga agcacgaaga ggaucuugac gaagacggau augcacugau cgaugcugcc      900

aaccagaugu acucugcugc cguuuuggca gcccucgugc ccgagggcau ggguuuuagc      960

gaagcuauga uggcgcggua ugcacagcau aagaaacaac uggccuuaua caagcagguc     1020

auugcuuccc ugccugacaa aacccagcga cacgagcuca aggaugccua ugcagacuac     1080

aucgagcaug ccgcucagaa uccacgcgau gccuucuaua aaagagugac aaaggugcuu     1140

gaaggggucg acucagcaga ugcgaggacu guuaugaccg cuaucaguca cgacgaguuu     1200

cuguugaagc aacggacacu aagcaacgga aaaauuccgu accagaugca gcagcacgaa     1260

uuugaugcca uaaucgagaa ucaaaaggca uacuaucccu ggcuggccac cccuaaccca     1320

guggaagcug acgccaaacg ccuccccuac uaucuggaug agcuuauugg cuuuagagua     1380

ccuuacuaug ugggcccaau ggucacggag aagcagucuc aguucgcaug gaugaucagg     1440

aaagcugacg gggaacugac ucccuggaau uucuucgaug ugguugaccg ugagaagaca     1500

gccaaccggu uuauacagcg aaugcaaacc acugacaagu acauuuuggg agaagaugug     1560

cucccgaaac auuccauccu guaucagcag uucgaggucu uaaaugaacu gaacaauauc     1620

agaguggaga agcacccucu aacaaccgag cagaaacaau ucauuuacca gaggcucuuu     1680

guagaccagc aacgcaagac gaucaccaaa aagaaccugc aggauacacu uguugcgaug     1740

ggugaauuug acaaggcccc cgauauuggc ggguuggcua aagaggacgu gaugauguca     1800

agccuggcaa cuuuugccga uuacaguaag auacucccag aagcuaucuc ugacccuacc     1860

cggcagaacg auauugagga caucaucgcc ugguccacaa uuuucgagga uacucagaua     1920

uuuuucgaca aacugaagac cgucgauugg cuuacaaccc aacagagaaa aaggcuggca     1980

agcauccgcu auacuggaug gggcaaguug ucgaaacgac uccugcagca gauucccacg     2040

ucuaagcaua caaaugugau ggcggccuua ugggcuaccu ccgacaacuu uaugcaaauc     2100

cugcaggacg aacaguucca ggagggucua uacgaugcca aucggcuccc agaagagacu     2160

cugacccccg acgaacaagu cgagaagauu cuugaugagg cauauuugag uccugcugac     2220

aaaaaggggg cccguaaagu gaucaagguu augaacgaua uagugagagc aaaucacggc     2280

guacugccag aacaggucuu ucucgaguca gccgacgauc cgcagaaaaa caagaggcgc     2340

agcguguccc ggucuaagca acuggaagag aaauacagac aggcugcgca gagcuucauc     2400

gacgaagccg uuaaggagga guuaaaugca cagauuguga acucagcuga auuuaaugau     2460

aggcuggucc uuuauuucac acaaaacgga cgagacaugu acaccggcaa acgcuuggau     2520

aucgagaauc ucgggaguua ucagauugac cauauccugc cccagucccu agugaaggac     2580

gauagccugg acaaccgggc ccucacugcc agagauauaa augcugaaau gucuaaccag     2640

uuuguaucag agagguucgg acuggguccu cgugcauucu ggcaacagcu uaaaaauaac     2700

ggcuugauua gugggcgcaa guuucggaac cuggugaugc agagagacga aaucgauaag     2760

uacgccccag gauucauuaa uaggcaguua guugagacac gacaaaucau aaaaacgguc     2820

gagcagauca uucaccggcu cuggcccgac auccagauug ugaccaucaa ggcgaaccug     2880

acucaccagg ugcgcagcga acuuggcuuu ccuaaaguca gagagcugaa ugauuaccau     2940

cacgcuuucg acgccuugcu cgcagccagg auaggucaau aucugcuaga acauuacgcu     3000

gggcugaagc cauuuuucac auauggcgag uuuaaacggg gagauaccaa gaaguacggc     3060

acaacuaacu uccuccgaag acugaccaaa gacaauaagg auaaaaaggg ggagcuuuug     3120

cccgaaguuu ccgacuggau ugauucuauc gacucgaucu auaacuacaa aaagauuccg     3180

gugguguggg agaauacgac caacaugagc gcacuguuug accagacaau cuauccucgc     3240

ccagauaggg acacuaccaa acguaaguua auucccacaa agcucgauaa accuacugac     3300

auauacggag guuacuccuc agccaagacc gcguauaugg cuguaaugcg gguccagacg     3360

aaaaagggca cagaauaccc cguguucaau aucccaguua guauugccuc ucaacugaac     3420

agaaucaccg aucagacuau gcuuaaacaa caggugaagc aguuuauuau caaugcagag     3480

cagagcgccu ccaagaaaaa gcuguaugaa ucaggggacg ucauacuaag caggguggcu     3540

cucggacagc gcuucacaga uaaaaacuac ggcgagguuu uccugaucuc uaauauggag     3600

gugcagaacc cucaauccuu guggcugggu cgagccaguc agcagagauu aacccgggaa     3660

cccgagggga augaaaagcc agagacuagc cucgcagaca uuuaucagca aauccugaaa     3720

cagguagaua gguacuuucu ugcuuucaac auucgccagc aacgucagaa gcuguugggc     3780

acccucgacc aguuuacauc ucugccgguc ggaagaguga acgaacagac gcggaccgau     3840

caaaaucaga gguccaucau agcccgcgug augauugucc uaggcaaggg gucacgagag     3900

gagaaauucc cugaauuugg acugagugcg cuuggcagac ucguucggac uggugggauc     3960

acacugagcg aggacgccca gaucauuuau cagucgccca ccggauuguu cgaaaggcgc     4020

guggcacuga aggauuuaug a                                               4041


<210>  3010
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3010
auggccaaga uccugggccu cgaccuuggg accaacagca uuggaugggc uguggucgau       60

auagagaaua acaaugaguu cacacugauc gagaaaggug uuagaauuuu ucaggaaggc      120

gugaagaucg agaaagggau ugaaggaucc aaggcagccg agaggacuac guaccggucu      180

gcgcgccgaa ucaaauaucg uagaaaguug aggaagauac aauuacuggg cguacuauca      240

gagaacgguu acugccccuu ccucaguaaa gacgaacugg auagcuggcg guauaagaaa      300

guguacccua ccaauaaguc cuuucuuaac uggcagaaaa caaaugagau ccagcuggaa      360

aacccauauu ucuaccgcgc uuugcucguc gagaagaagc ugaaucugaa cauugaggaa      420

gacagauuua aaaucgggag ggccuucuau caccucgcac agcggcgcgg auuuuuaucu      480

aaucgaaugg agggcacuaa ggaaaacgau ggcaccguga uuaaaucgau cacagagaua      540

ucaaccgcca agggggacaa aacucuggga caauacuucu acgaaagaua uaagaaaggu      600

gagaagauua gggauacaua cacccaucgg gaggaacacu aucuugagga auuuguucgu      660

aucugugagu uccagcaguu ggacaauaag uuugcugaaa aagugcgcaa ggcaauuuuc      720

uaccagagac cgcugaaaag ccaaaagggc cuaaucggga aaugcgucuu ugagcccaag      780

aagaguaggu guuccguguc ucggccugag uucgaagagu aucgaaugcu cugcuuccug      840

aacaauauca aaauuaagac gccaagcgau gaaaaccuuc gcuuucugac ugacaacgag      900

aaagaaaagu ugaaacccca guucuuuaga aaguccaagg aucauuucga cuuugaggau      960

cucguaaaac agcuggcccc uaagaaucag uucaaauuuu acaagucaag ggacauauua     1020

ccaggaaacu ggcuguucaa uuauaguaug aacaccacag uuagcggcug ucccaucucu     1080

gcucggcuca aagaccuuuu uggugagaau uucauggaua uuaaguuuga cuacauccgc     1140

gaaaaggaug ggaaaaaguc cgugauagac auucacgaua ucuggcaugu ccuguucacu     1200

uacgacucag aggaaaaauu gaaggaguuu gccagaaaca ggcuaaaucu ggaugacauc     1260

aaaauuaagg aguuccaacg uauccagcuc aaaaaggaau augcggcacu gagccuuaag     1320

gccauucgga aaauacugcc uuucuugcga gagggacuca ucuacaguca cgcuguguuu     1380

auugccaaca uggaaaccgc aaucccaucu gagaucugga auaacuauac aaauagaaac     1440

auuauaaaga augaaaucua caacauuauc uccaaucaga ccgaggauaa acaagugguc     1500

gacauuguua acggcaucau aaagagcucg aggaucgaga acucugugug guccaaugaa     1560

gcuguagagu ucuuuaaaaa ggaucugaac ucaaagauua aaaauuauua cggcaucaag     1620

acguauaacg acuucccgga agagaaaaag auucgcaucg aaacuuacgc cuuuaauuua     1680

uucaaacagc agaugaugaa gaacuuuggg cggggagagu ucgcggccgu gcagagaaua     1740

gacgagaggc ugaagcuauu ucucacagau aauuucaaca uugaccccau ccugcuuaaa     1800

aagauuuacc acccuagugc aaucgaaguc uaucaaccac ccgugcgcaa aaaugauggu     1860

aaguuguacc ugggcagccc uaugguuucc ucuauacgaa acccaauggc uaugcgggcc     1920

cuccaucagc ugcguaaacu uaucaaugag cugauuagaa aggaauugau cgacagcaac     1980

accaagguga auauugagau ggcuagggaa cucaugaacg ccaaugagcg caaagcauau     2040

cagaacuggc agaacgaucg ggagaagauc agaaaagaau acgcccugaa gauaaaacaa     2100

cacuuagacg ucgauaagga gcccucagac guggauauuc ugaaauauca gcuaugggaa     2160

gaacagaauc auaagugcau cuacacuggg aaggagauca acauuaguga cuuucucgga     2220

uccaauccga aauucgauau cgaacacaca auuccuagga gcauaucuuu ugacaacuca     2280

cagaguaaua agacccugug ugagaacuau uucaaucgaa gcguaaagaa gaaccgcauc     2340

cccgcugcgc uuccaaauca cggcgaaauu aucgagcggu uggaaaacug gaaacugaag     2400

uacgaggagc ucgaaaagca aauucaguac gcaguuagac aggccaaagg ugcuggggau     2460

aaggacucca aagacagggc cauccagaag cgucauaaac ugaaguucga guaugauuac     2520

uggaagaaua aauaugaccg cuuugugaug gaagaugucc cugagggcuu caagaacucu     2580

caauuagugg acacgggaau aaucaccaaa uacucgcgga uguaucugaa uacacuuuuu     2640

gauaaggucu acacugugaa aggcaacacc guugcagacu ucagaaaguu gugggggauu     2700

caggaugaau augagaagaa agccagggug aaucacagcc aucacugcgu agacgcuauc     2760

acaauugccu guaucacuaa guccaacuac gagaaccucg caaaauuuua ccaugauugg     2820

gaggagcugc acgucaaggg aauagaccag cuacccccaa ugaauaaacc cuggaagacc     2880

uucacagaag auauuaaggc gauccaauau gagauccugg ugucacacua caccccugac     2940

aaccucccaa aacagaguaa gaaaauucug cgaaagaaag gucggauaca gaagaaugaa     3000

aacggcaauc cgaucuaucu uaaaggggau acuguucgcg gaucuuugca ucagcaaacg     3060

uuuuacggcg cuauugagaa gacagaggug aacaagaaag gugaacccga gaaggucauc     3120

aaauaugugg uaagaaagag ccuggacgug uuagacgauu ccucaguuaa aaauauuguc     3180

gacgauaucg ugaaggaaau agugcagaac gccaggauca aggagaaaga cuucauuaag     3240

gagcucgaaa uccagaaacg gaagcuggca aaagccgagg aaaauaaggu cguucagauu     3300

caagcugaga ucgauaaacu uaacaaacag guggccgacc uguacaccau gccuaauaag     3360

aacgggaauc caauacccau uaaaaaggug cgaaucuuuc agacuagcgu aaccaacccu     3420

auccacuuga aacagcaaag agauaagucu cagaagacau ccaaaccaca uaaggaguuc     3480

uauuacgcag cuaaugacag uaacuacaug auggccauuu augaaggcaa agaugagaag     3540

ggaaaaguca agcgcgacuu uaaacuccug aauaaccuag aagcgggcga guucuucaag     3600

uacagcgugc acaaggaucu gaaagaaaag aaccucgaga auaucaggaa cguuauaccc     3660

gacaaagugu cugccgggaa gauugaaauc ccgcugaaag cuauuaucaa gaccggaacu     3720

auggucauac uuugggaaaa uuccccugac gagguguggg auuugacaau uucagaaguu     3780

gacaagcguc uguauaaagu gguagguaug agugucaacc ggaucaagag cgaugugaaa     3840

acguuugagu ucggcaauau cguguuaaga cacaacaagg aagcaucgcc agccacccuc     3900

cugaaaauuc aggacgggga guuuaagucc gaugaaaauu acaucgcuca aaggaaacuu     3960

ucucauaacc aguucaaugc ccuggucgag ggaguugacu uuagccuauc agugcucggc     4020

gagauuaagc gccugaagug a                                               4041


<210>  3011
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3011
augggcgagg acgggaagcc cgugagaguc aaaggaaaga ccgccaucgg ugcuaggcug       60

uuccaggaag gccacguugc agccgaucgg cgcauguuuc gaacaacucg uagaaggcuc      120

aaccggcgca aauggagacu uaagcuguug gacgagauuu ucgauccuua cauaacgcca      180

guggacagca ccuuuuucgc gagguuaaaa caauccaauc ugucuccgaa ggauucacgg      240

aaggaauuua aagggaguau gcuauucccc gaccucacag auaagcagua ucaugaggac      300

uacccuacua ucuaucaucu gcgccgagcu cuuaugaccg aagauaaaaa guuugacauu      360

agaaugguau accuggccau ccaccacauu gugaaauaua ggggaaacuu cuugaauagc      420

gcaacagucg auaccuuuaa ggccuccaag guggacuuca aaaagucuuu uaaccagcuc      480

aaugagcugu acucggcuau caacccagag gaugcauucc agguugacau gaauaaauca      540

gacgaaacug gccggcuguu ucucgauagc aguauacgua aguucgacaa acaaaagcag      600

aucuccaaau uaauucccgu ggucguggag gauaaggaag ccaagaaacu gaacgguaag      660

aucgcucuug agauuacaaa agccaucuug ggguauaagu cuaaacugaa uguaguuacc      720

cagacggagc cugaagaccc aaagaagugg gcggugaaau ucgaugacga ggauauagac      780

agcaagcuaa acgauauucu ccccgaaaug gacgagaauc agcaauccau cgucgaaauu      840

auccaggauc uguacucaca ggugacucuu aaccagaucg ugccuaaugg aaugagucug      900

agcgacucua ugauuaaaaa guacaacgac caucacgagc acuugaaacu cuauaagaag      960

cugauagaua auaucuccga caccaaaaag cgcucagcau uaaaaacagc cuacgauaag     1020

uaugucggcg acgauggcaa aacuauugag caagacgaau uuuacagcaa cguuaagaag     1080

aaccuggaug acagugaucu cucuaaacag aucgcugacc uuauagaggc cgaaaaguuc     1140

augccaaaac agagaaccuc ccaaaauggg gugauuccgc aucagcugca ccagagggag     1200

uuggaugaaa ucaucgagca ucagagcaag uauuaccccu gguuugcaga ggcuaacccu     1260

aauaaacacg accuacaccu ggccaaguau aaaauugaag aacucguagc guuccggauc     1320

ccauacuacg ugggacccau gauuacaccu gaagaucaag agaagucguc ucagaccaua     1380

uuugccugga ugaagcgaaa agaaccaggu cagaucacgc ccuggaacuu cgacgagaag     1440

gucgaccgca uggaguccgc aaauagauuu auuaaaagga ugacuacaaa ggauaccuau     1500

cugaucggcg aagacgugcu uccggaucag ucacuguugu acgagaaauu caagguucuc     1560

aacgaacuga auauggugcg ggucaacgac cauccuuuaa aggugaguga gaaacaagcu     1620

aucuaucgcg aucuguuuga gaaucagaag agaauuagcg uaaagaaccu acagaauuac     1680

guuaagucca aaucugggcu ccccacugga ccaaagauaa gcggccuguc agacccugau     1740

cacuucaaca guucccuugg uacauauaau gacuuuaaga aaauguucgg ggauaaggug     1800

gacgaucccg acuugcagga ugacuuugaa aagaugaucg aguggagcac cauuuucgaa     1860

gacaagaaaa uccugagggc caagcucgau gaggucgcuu ggcugucuga cacgcaaaag     1920

aacgaguuca aagagauucg uuaccaggga uggggccggc uuucaaagaa acuguugacc     1980

ggcaucgugg augggaaugg acagcgaaua aucgacgagc ucuggaacac aaacaagaau     2040

uuuaugcaga uucaaaguga ugacagcuuc gccaaaagaa uccaugaagc aaacgccaau     2100

cagaugaagg cuacugaugu cgaggacgug cuggcggaug cauauaccuc cccacagaac     2160

aaaaaggcca uuaggcaagu ugugaaggua gucgacgaua uccagaaagc uauggguggc     2220

guggccccca aguuuauauc uauugaguuc acacgcucgg aagaccggaa uccuagaagg     2280

acuaucagcc gccagcgaca guuagaaaac acccugaaag acacagcaca agcccuaguu     2340

aaguccauua auccagauau ccucucagag cuggacaacg cuaguaaauc uaagaagggg     2400

cuuaccgauc gguuguaccu guacuuuacu cagcucggaa aagacaugua uacgggcaag     2460

ccgauaaaua ucgaugagau uagcaacuac gacaucgauc acauuuuccc ccaggccuuu     2520

aucaaagacg auucccugaa uaaccgugug uuagucucaa aggcagguaa uaacgggaaa     2580

agcgcgaaug ugccucugaa acuuuucggc gcuaagaugg gaggcuauug gaaacaguug     2640

gccgacgcag ggcucauagg acaaagaaag cugaaaaacc uacagacaaa cccagauacc     2700

auuucuaagu uugccaugca ugguuucauc aggcgccagc ugguagaaac uucccaggug     2760

aucaaacucg uugcuaauau ucugggcgac aaguaccgga acgaugacac caagaucauu     2820

gagauaacag ccagaaugaa ucaccaaaug agggacgaau uuggguucau caaaaaccga     2880

gagauuaaug auuaucacca ugcauuugac gcuuaccuua ccgccuucuu gggacacuau     2940

cuguaccauc gcuacaucaa guuacggccc uauuucgucu acggcgauuu uaaaaaguuc     3000

agagaagaca aagugacuau gaggaacuuu aauuuccucc acgaucugac agacgauaag     3060

caggagcgua uugcggacac ggagaccggu gaagugaucu gggaucgcaa gcggaguaua     3120

cagcaacuua gagacgucua ucacuacaaa uuuaugcuga ucagccagga gguuuucucu     3180

uugaacaagg aaauguuuaa acagaaacuc uucccugcca cucuggcuaa aaaguccaaa     3240

cuaauucaga ucaagaagga uaaaccagug gacauuuaug ggggcuacuc aaauaacaca     3300

gaugcauauc ugaccguggu aaagcucaaa ggaaguaagg aggacaaaua caagaucguc     3360

ggcgugccca ugagggcccu ggacaaucuu aagcaugcug agaaagaaag cccgcaaucg     3420

uacacagccg aguugcgaaa ggcacuggaa ccucaguuua cuaaaauaaa gaaaaaccgg     3480

aagaccgggg agguuuccaa gguggucacg aauuucgaua uuguguuagg acgccucuuu     3540

uauagacaga ugguuaucga cgguucugag aaaagccuga ucgcgucaag uguguaccag     3600

uauaacgcca agcaacuugu acuguccgau caggcuauua ggaccauauc ugaccaggaa     3660

ggcaucgaug aguucgaacu aagcucccag uacgacaaag ucuaugauga gauugugcaa     3720

aagguggaaa aacuccugcc aaucuuccag caccggcauu uucaggagcg auugaauaac     3780

gggaagucac uguucgucaa cuuacccguu cacgacgugu acgaaggaaa uaagcucgag     3840

cagguaggca aaaagcauau uaucaacagc auacugggug ggauccacgc caaugcagau     3900

agaccugaca uugaucuuuu uggcaucaaa aaccugggac aauucaguuc uacauccggc     3960

auuaauuuga gcucggacgc uaucauaugc uaucagucac ccacugggcu cuuugagcgc     4020

aggguggucc ugaaggaccu auga                                            4044


<210>  3012
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3012
augaccaaga gagaggaacc cuacaacgug ggccuggaca ucgggagcuc cucugucgga       60

ugggccguug uggauaauaa cuaucaccuc cuuaauauua aaaagaacaa ucuguggggu      120

gcuagguugu ucaaagaggc agaaacagcc cagguaacuc ggggccaucg cucaaugcga      180

cguagauaca ggcggcgcag aaacagguua aauuggcugg acgagcuauu ugcggaugaa      240

cucgcuaaga uagacccuag uuuccugcuu cggaugaaaa acagcugggu guccaagaag      300

gagucuacgc gcaaacgaga uccauauaau cuguuuaucg acgagaagua caacgauguc      360

gacuauuuca aucaauaccc gaccauuuuu cacuugagaa aagaacucau cacagaggau      420

aagaaagugg acauuaggcu gaucuaucug gccauacaua acaucauuaa guaccggggg      480

aauuucacuc ucgaaaacca gaauuuugau aucucgcagu uaucaagcaa cuucagucag      540

caaauuuccg acuuuuucgc acuguuuucu gauuucggag uuaucaugcc cgaggacuuu      600

gacccugaua agauaagcga cauucuuuug aacccaaauc ugucccccuc aggcaaagug      660

agugaggcca ucgcuaccau uagcccuaag acaaacguca aagcaaagau caaaaucauu      720

cuacuccugc uuguggguaa uaacggggau cugaagaaau uguucgaccu cgaaaccacu      780

gagaagauag ccguaaagcu gucuucccgu cacaucgauu cagaauuacc aauuauccug      840

agcgagcuca augaacagca ggagaacaua auuacaaucg cuaauaguau cuucggaucu      900

auuauccuua aagacuuucu gggcgaugag accuccauua gcgccgcgaa gguuauaucg      960

uucgaagacc auaaacagga uuugcaaaag cuaaaaacga uguggcgcga gacuucuaac     1020

aaggaagcag ugaaggccgg caaaaaggcu uacgaggacu auaucgggca cgaagauucc     1080

gagaccuuuu acaaaaagau uaaaaaguuc cuggagaagg cccagcccgu cgaccucgca     1140

aauaaagcuc uggccgaaau cgagcuugaa aacuaucugc cuaagcagag aaauaggaac     1200

aauacaguga ucccauacca guugaacgag aaugaacuca uugcgauacu ggaccaccaa     1260

gagaaauauu acccguuuuu aaaggagaac cgggauaaaa uccugucacu acucacuuuc     1320

cgcauucccu auuacguggg accucugcag gacaguaaca auaaccgauu uagcuggaug     1380

accagaaagg ccuccggugc aaucaggcca uggaauuucu cugaaaaggu caacguugag     1440

cagagcucaa augauuuuau uaaacggaug cgcaguacag acaccuaccu uaucggcgaa     1500

cccguguugc cuaagaaauc ccugauauau caaugcuacg agguacucag cgagcugaac     1560

aauacgagag ugaaggaugg gucuucaaac ccaaaaaggc uugacgucac uaucaagcag     1620

cguauuuaua augaaaucuu caaaaaccag aagaguguga gcguuaaggu gcugcagaau     1680

ugguugauua aagaguccua cuuuaagucu cccgaaaucu cgggacucgc ugauaaaaag     1740

aaauaucuga gcuccuuauc aacauacaua gacuucaaga agauuuuugg ccaacgguuc     1800

gucgaugacc cggugaacag uccucagcug gaggaacuag ccgaguggcu cacccuguuc     1860

gaggauaaaa agauccuuuu gauuaaacug caaaauucua aguauagcua cgaccaggcu     1920

acuaucaaca aacucuccac aaugcgauac caggguaccg ggaagcuguc aaagaaauua     1980

cugguagauc uuaagacgac caaaaagagc auaggaaaau cuggcgccga auccuugagu     2040

auccucgacc ugauguggag cacaaaggac aauuuuaugc agauuaucca ugaugcagac     2100

uauacuuucg agcagcagau uaaggaauuu aacuacgaua ccgaggacga acuaacaccc     2160

cuggagaaag uugccaaccu ccacggcucu ccagcucuga agagagggcu uaaucaaucc     2220

aucaaagugg ucgcggauau agugaaguuc augggacaug acccugagaa aauuuuuauc     2280

gaguucacua ggucagauga cuuuaguaag uugaccauca gccgcuaucg gagaauuaaa     2340

aagcaguacc uggaaaucgc aaaggccauu aaaaagauac ccgcugaguu caaagauauc     2400

aaggaauauc agacacaguu agaagagaac aaagguaagc ucgccucgga gaggcugaug     2460

cuuuacuuuc ugcaaugugg ccacuccuug uauucuaaua agccaauuga ccucaacaug     2520

aucaauagcu caaaauacca cgucgaucau auucugcccc agaguuacau caaggacgau     2580

ucccuagaaa acaaagcacu ggugcucgcc ucugagaaug aaaacaagau agacaauaug     2640

cugaucagcc acgacauuau cgcuaccaac cuuccucgcu ggcaggccuu gaaagaucaa     2700

aaucugaugg gguccaagaa guucgcagac uuaacucgaa cgacaguuac cgagaaccag     2760

aaaaagggau uuauucagcg gcagcucgug caaacuucac agaucguaaa gaauauaacc     2820

cugauucuua acgaucugua uaagaauaca agcugcaucg agacccgugc gacucuaagu     2880

ucugaguuca gaaaggcuuu uuccaacuuc gacgaaacaa cguaccauua ucaguuccca     2940

gaauuuguca agaacaggga ugugaaugac uuccaccaug cccaggaugc auuucucgcc     3000

uguguuaucg gcgaguacca acugaagaaa uauccgaagg acaacuugcg ccugguguac     3060

gaucaguaua gcaaauucuu agacucgcuc aagaaagaua cccggaagaa gaaugguaga     3120

augcccaggu acacucagaa cggguuuauu auaggcucaa uguucaaugg aaaaacauac     3180

gucgacgaca acggcgaaau cauuugggau cagaagauca aagagucuau ucgaaagacc     3240

uuuaauuauc accaauucaa cgugguacgc cagacaaucg agcagcacgg gaaacuguuu     3300

aaugacacua uacaaccuca uagugaucgg uacaaacuua ucccacugaa aaccaacaga     3360

gaccccgcua uuuauggagg uuacaauaac gauaauaacg ccuauuccgu gguuuuggac     3420

gucgauggca agaagaaaau caaugggauu ccuaucagga uagcaaacca gcucaagagc     3480

gacgaacugg aucuaucuag cuggcuggag aauaacauua aacacaagaa accaaugacg     3540

auccuuaucg acaaggugcc caaguaccag cguauuauca acgaagagac cggagaucuc     3600

cugauaacau ccgcuaauga agugauuaac aauguccagu uguuccugcc gucaauguau     3660

acugccuuaa ucagucuccu ggacagcacc aaaacagaga uguacucuaa acuucugucc     3720

aacuacgagg cgaauauuuu gaucgauaua uaugacuacc uccugacuaa acuaaagaac     3780

aauuauccuc uguaccgcaa agaaugggcc aaacuugcug agcaucggga cgauuuuauu     3840

gaaucagacc ucguuaccca agcaaguacg cugcagcagu ugaucaaguu caugcacgcc     3900

gauccaagca acgugaauuu aaaguucggc aacuuuaagg guaauagauu cgggaggaaa     3960

aacggcaaua ucaagcuguc caaaacagac uuuauuuaug agucucccac cggacucuuc     4020

aagucgauca agcacauuga uuga                                            4044


<210>  3013
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3013
augaaaagcg agaaaaagua cuauaucggc cuggacgugg ggaccaacuc cgucggaugg       60

gccguuacag augaguucua caauauucuc agagcuaaag guaaggaccu uuggggcgug      120

aggcuguuug agaaagcaaa gacugccgcg gauacgcgga ccuuccgcuc ugggcgacgu      180

agaaacgaca ggaagggaau gcgguugcag auauuacgcg aaaucuuuga ggaugaaauu      240

aaaaagguag acaaagauuu cuaugacaga cuggaugagu caaaguuuug ggcugaggac      300

aaaaagguga guggcaagua cagccuauuc aaugauaaaa acuuuuccga caagcaauau      360

uucgaaaaau uucccacaau cuuccaccuc aggaaguacc ugauggagga acaugguaaa      420

gucgauauuc gguauuacuu ucuugccauc aaucagauga ugaagcgccg agggcacuuc      480

cugauagacg gacagaucuc ucaugugacu gacgauaaac cuuugaagga gcagcucauu      540

cugcugauca acgaccucuu aaagauugag cuggaagagg aacuuaugga uucgaucuuu      600

gagauauugg cagacguuaa ugaaaagaga accgauaaga aaaacaaucu gaaggagcua      660

auuaaaggcc aagacuucaa caagcaggag ggcaauaucc ucaacucaau uuucgaaagc      720

aucgugacag ggaaggccaa aaucaagaau auuauaagug augaggacau ccuggaaaag      780

auuaaggagg auaacaaaga agacuuuguc cuuaccggag auuccuacga ggagaaucug      840

caguauuucg aagagguguu gcaggaaaac aucacucucu uuaacacacu gaagucuacc      900

uacgacuucg uaauauuaca aagcauucug aaggguaaau ccacgcucuc agacgcucag      960

guugagaggu augaugaaca caagaaagac cuugagaucc ugaagaaagu gaucaagaag     1020

uacgaugagg acggcaaauu guuuaagcag gucuucaaag aagauaaugg gaacggauau     1080

gugaguuaca uuggcuauua ccuaaauaag aacaaaaaga ucacugccaa aaagaagauu     1140

agcaauauag aguuuaccaa auacgugaag gguauccugg aaaaacagug cgacugugag     1200

gaugaggcag ucaaguaucu ccuggggaaa auugaacaag agaacuuccu ucugaagcag     1260

aucucuucca ucaauucagu uauuccacau cagauacacu uguuugaacu cgacaagauc     1320

cuggagaacu uagcuaaaaa uuacccgagc uucaacaaua agaaagaaga guuuacaaag     1380

auugagaaaa uccggaagac uuucaccuuu cguauucccu auuacguggg accucugaac     1440

gauuaucaca agaauaacgg cggcaaugcc uggaucuucc gcaacaaagg ggaaaagaua     1500

agaccaugga acuucgagaa aaucguagac cuacauaaga gugaagagga auuuauuaaa     1560

aggaugcuca aucaaugcac auaccugccc gaggagaccg ugcuuccuaa gucuuccauc     1620

uuguauagcg aauacauggu ccugaacgag cucaauaacc ugcggauuaa gggaaaacca     1680

cuugauacgg acgugaagcu gaaauugauc gaagaacucu ucaagaaaaa gacuaaaguu     1740

acacugaagu cgauacgaga uuacaugauu cgcaauaacu uugcggacaa ggaagacuuc     1800

gauaauucug agaaaaacuu agagaucgug uccaauauga agucauauau ugacuuuaac     1860

aauauccugg gugauaaauu cgaacuagag auggucgaag accucauaga gaagaucacc     1920

auucacacug gcaacaaaac acugcuuaag aaguacaucg aggaaaccua ucccgauuug     1980

aguagcuccc agauucagaa aaucauaaau cugaaguaca aagacugggg gagacucucu     2040

aggaagcugu uagauggaau uaaaggccgg aacaagacgg acgaugugaa uaccguaauc     2100

aacuuuaugc gcaacagcuc agacaaucug augcagauca uugaggguaa gaacuauagu     2160

uucaaugaau acaucgauaa ccuuaggaaa aaguauauuc cucaagagau auccuacgaa     2220

guuguggaga auuuguacgu cagcccaucu gugaaaaaga ugaucuggca ggucauuaga     2280

gugacagagg aaaucacuaa aguuaugggg uaugacccgg auaagauuuu uaucgagaug     2340

gcaaagucag aagaggagaa aaagaccaca auaagucgua aaaacaagcu ccuggaccua     2400

uacaaagcca ucaagaagga cgaacgggau agccaguaug agaaacugcu cacuggccug     2460

aauaaacuug acgaauccga guugcgaucu aaaaagcugu acuuauauua cacccagaug     2520

ggaagagaua uguauacagg cgagaaaauu gaccucgaua agcuguucga cucgacccau     2580

uacgauaaag accacaucau uccccaaagc augaagaagg augacuccau cauaaacaau     2640

cuugugcugg uaaacaaaaa ugcuaaccag acuacgaagg ggaauauuua cccuguccca     2700

ucaaguauca ggaacaaucc cgauaucuau aacuacugga aauauuugau ggaaaaggaa     2760

uuuauuucua aagagaagua caaccgccuc auacggaaua caccucugac caacgaggaa     2820

cuaggagugu ucaucaauag acagcugguu gagacuaggc agagcaccaa ggccauuaaa     2880

gaacucuuug agaaguucua ucaaaaaucc aagaucauuc cagugaaagc aucacuggcu     2940

agcgaccuuc gcaaggacau gaacacauug aagucucgag aggucaauga ucugcaccau     3000

gcccacgacg cguucuuaaa caucguggcc ggugauguau ggaaucggga auuuaccucc     3060

aaccccauaa auuacgugaa cgagaaucgu gaaggcgaca aaguuaagua uagucucagc     3120

aaauacuuca cuagaccgag gaagucuaaa gggaagguca ucuggacacc ugagaaggga     3180

cgcaaacuga uuguggauac gcuuaacaag cccuccgugc ugaucucaaa ugaaagucau     3240

gucaaaaagg gcgagcuauu uaacgcaacc auuagcggua aaaaggacua caaaaagggg     3300

aagaucuauc ucccacugaa aaaggaugac cgguugaaag auguuucgaa guacggcgga     3360

uauaaagcua uaaauggcgc cuucuuuuuc cugguggagc acacuaaguc caagaaaaga     3420

auuaggucua ucgaauuauu uccucuccau cugcuuagca aguucuacga ggacaaaaac     3480

acagugcugg auuaugcuau caauguauug cagcuccagg accccaagau uaucauagau     3540

aaaauuaacu accgaaccga aaucauuauc gacaacuuuu cauaucugau aaguacaaag     3600

uccaaugacg ggucuauuac ugucaagcca aacgagcaga uguacuggcg cguggaugaa     3660

aucagcaauc ugaaaaagau cgagaacaaa uacaagaaag acgccauucu aaccgaggaa     3720

gaucggaaga ucauggaguc cuauauugac aagauauacc aacaguucaa agcaggaaag     3780

uauaaaaaua gaaggacgac cgauacaauc auugaaaagu acgagaucau cgaccuugau     3840

acucucgaca acaaacagcu guaucaauug cugguugccu uuauuucauu aagcuacaag     3900

accaguaaua acgcugugga uuucacaguc auaggucucg gcacugaaug ugggaaaccc     3960

cguaucacca aucugccuga caacacguau cuuguguaca agucuauuac aggaaucuac     4020

gagaagcgca uucggaucaa auga                                            4044


<210>  3014
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3014
augaagaaac ccuacaccau cgcccuggac auuggcacaa acagcguggg gugggucguu       60

gugacugaug acuauagagu accuacgaag aaaaugaagg ugcucggaaa uaccgagagg      120

aaaacaauaa agaagaaccu uaucggugcu cuguuguucg auuccggcga cacugcagaa      180

gggacccggu uaaaacgcac agcccgacgu agauacacca ggcggaagaa ucgccugaga      240

uaucuaaaag agauuuuuac ugaagagaug gcgaaggucg augacggauu cuuucagagg      300

cucgaagauu cuuucuacgu gcuggaggac aaagagggca acaagcaccc aaucuuugcu      360

aaucuugccg augaaguugc auaucauaag aaauacccga caauuuauca ccuucggaag      420

gaguuggugg acaaccccca aaaagccgau cuccgccuga ucuaccuggc ugucgcacau      480

auaaucaagu uccgagguca cuuucucauu gaagggaccu uaucaaguaa aaauaacaau      540

cugcaaaaga gcuucgacca ucuuguggau acguacaacu ugcuguuuga ggagcagaga      600

cuacucacug aaggaaucaa ugccaaagag cugcuuuccg cugcccuguc uaagucgaag      660

agguuggaaa accucauuuc acugaucccu ggccagaaaa agaccggcau auucgggaau      720

auuaucgcgu uaagccuggg acucacacca aacuuuaaag caaauuucgg ucuuaguaag      780

gacguaaaac ugcaguuggc caaggacacu uaugcugaug accuagauuc ccugcucgcc      840

cagauuggcg accaguacgc agaucuguuu cuugcugcca agaaccuguc ugacgcgauc      900

uugcucagcg auauccugac cgaguccgac gaaauuacac gggcccccuu aucagcaagu      960

augguuaaac guuaucgcga gcaccacaag gaucugguga cccuaaaaac gcucauaaag     1020

gaccagcugc cugagaaaua ccaagaaauc uuccuugaua agacuaagaa cggguaugcu     1080

ggauacauug agggccaggu cagccaggaa gaguucuaua aauacuugaa gccaauccug     1140

gccagacucg acgguucuga accccugcuu cugaaaauag acagggagga uuucuugcgg     1200

aagcagcgaa cauucgacaa uggguccauu ccucaucaaa uccaccucga ggaacugcau     1260

gcuaucuuac gcagacagga gguguuuuac ccauuccuga aagauaacag gaagaagauu     1320

gaaucacuac ucaccuuucg gaucccguau uacgugggac cccuggcccg cggccacagc     1380

agauucgcau gggucaaaag gaaguuugac ggcgccauuc guccuuggaa uuucgaggaa     1440

auaguugaug aggaggcuag ugcgcagauc uuuauugaga aaaugacuaa gaacgaccuu     1500

uauuugccaa augaaaaagu gcugcccaag cacucucucc uguacgaaac auucaccgua     1560

uauaacgagu uaacgaaagu gaaguacgca accgagggga ugacacggcc ucaauuucug     1620

uccgccgauc agaagcaggc uaucgucgac cuuuuguuca aaacuaaucg aaaggugacc     1680

guuaaacagc ucaaggaaaa cuauuuuaaa aagaucgagu gcugggauag cguggaaauu     1740

acaggagucg aggacucguu caaugccucu cuggguacuu accaugaucu acugaagaua     1800

auccaagaca aagauuuccu cgacaaccca gacaaucaga agauuaucga agauauuauc     1860

cugacccuua cauuguuuga ggacaaaaag augauaucca aaagacugga ucaguacgca     1920

cacuuauucg acaagguggu acucaacaag cuggagaggc aucacuauac cggcuggggg     1980

cgccuuucag gaaaacugau caauggcauu cgggauaagc agagugguaa aacuaucuug     2040

gacuuucuca aggccgaugg guucgcuaac agaaauuuua ugcaacugau ucaugacagc     2100

gaacuauccu ucaucgauga gauagccaaa gcacagguua uuggcaagac ggaauacucu     2160

aaggaccugg ugggaaaccu cgcgggcagc cccgcuauca aaaaggggau uucacagaca     2220

aucaaaauag ucgaugagcu ggugaagauc augggauauc uuccgcagca aauugucauc     2280

gaaauggcca gggagaacca gaccacugca caggguauua aaaaugcccg ccagcgaaug     2340

cggaaguugg aggaaaccgc uaaaaagcug ggcaguaaca ucuuaaagga gcacccugug     2400

gacaauuccc aacuccagaa cgauaaacgu uaccuguauu accuucagaa ugggaaggac     2460

auguauacag gagacgaucu ggacauagau uaccuaagcu cuuacgacau ugaucacauc     2520

aucccccaau cauuuauuaa aaacaauagu aucgacaaca agguucucac cagccagggc     2580

gccaauagag guaaacugga uaacgugcca uccgaagcaa uuguaaggaa gaugaagggg     2640

uauuggcagu cuuugcugcg cgcuggcgcc auaucgaaac agaaguucga caauuuaacu     2700

aaagcggagc ggggaggccu cacacaaguc gauaaggccg gguuuaucca gagacagcug     2760

guggaaacga ggcagauuac caaacauguu gcucaaaucc uugacagccg auucaacacu     2820

gaguuugaug accacaauaa gcgcauucgg aaggugcaua ucauaacacu gaaauccaag     2880

uuggucucag acuucagaaa agaguuugga cucuacaaga ucagggauau uaaccacuau     2940

caccaugcac acgacgccua ccugaaugcu gugguagcca aagcaauccu agguaaguau     3000

ccucagcugg cgcccgaauu uguguacggc gauuauccaa aguacaacag uuucaaagag     3060

cgucagaagg ccacccagaa aaugcuguuu uacucuaaca uucucaaguu cuuuaaagac     3120

caagaaagcc ugcauguuaa uuccgauggg gaggagaucu ggaacgcuaa uaagcacuug     3180

cccauaauua agaacguccu gucaaucccu caggugaaua ucgugaaaaa gacagaaguc     3240

cagacuggag gcuucuauaa agagagcauu uuaucuaagg guaacuccga caaacucaua     3300

ccacgcaaga auaacuggga uacccggaaa uacgggggcu uugacagucc gacgguugcc     3360

uauagcgugc uggugaucgc aaagauggaa aagggaaaag cuaagguacu uaaacccguc     3420

aaggagaugg ugggcauuac caucauggaa agaacagccu ucgaggagaa uccuguugug     3480

uuucuggaag caagggggua ccgagagauu caagaacauu ugaucauaaa acucccaaag     3540

uauucucugu ucgagcuaga aaacggacgg cgcagacugc uugccuccgc uucagagcuc     3600

cagaagggca augagcuguu uuugcccguc gauuacauga cuuucuuaua ucuggcggcc     3660

cacuaccacg aacucaccgg uaguagcgag gacgugcuga ggaaaaagua cuuuguugau     3720

cggcaucuuc acuauuucga cgauaucauu cagaugauca acgacuucgc agaacgacau     3780

auucuggcuu cguccaauuu ggagaaaauc aaccacacau accauaauaa cucugaccua     3840

ccugugaacg aaagagccga gaauauaauu aacguauuua cuuucgucgc ccugggggcu     3900

ccagcagccu uuaaguucuu ugaugcgacc aucgaccgca aaagguauac gagcacaaag     3960

gaggugcuca augcuacccu gauccaccag ucagugacug gacuuuacga aacacguauu     4020

gauuuaaguc aacugggcga gaacuga                                         4047


<210>  3015
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3015
augaaaagcg agaaaaagua cuauaucggc cuggacgugg ggaccaacuc cgucggaugg       60

gccguuacag augaguucua caauauucuc agagcuaaag guaaggaccu uuggggcgug      120

aggcuguuug aaaaagcaaa gacugccgcg gauacgcgga ccuuccgcuc ugggcgacgu      180

agaaacgaca ggaagggagu acgguugcag auauuacgcg aaaucuuuga ggaugaaauu      240

aaaaaggugg acaaagauuu cuaugacaga cuggaugagu caaaguuuug ggcugaggac      300

aaaaagguca guggcaagua cagccuauuc aaugauaaaa acuuuuccga caagcaauau      360

uucgaaaaau uucccacaau cuuccaccuc aggaaguacc ugauggagga acaugguaaa      420

guggauauuc gguauuacuu ucuugccauc aaucagauga ugaagcgccg agggcacuuc      480

cugauagacg gacagaucuc ucauguuacu gacgauaaac cuuugaagca gcaacucauu      540

cugcugauca acgaccucuu aaagauugag cuggaggaag aguuuaugga uucgaucuuc      600

gaaauacuug cagacgugaa ugagaaaaag accgaaaaga agaacauuuu gaaagagcug      660

aucaaugccc aggauuucaa caagcaggag ggcaagauuc uauacucaau cuuugaaagc      720

aucgucacag gcaaagcuaa gauuaaaaau auaaucagug acgaggauau ucucgaaaag      780

aucaaagagg acaacaagga agauuucgug cugaccgggg acuccuauga ggagaaucuu      840

caguacuuug aagagguacu gcaagaaaac auaacuuugu ucaauacacu caagucugca      900

uaugauuuug uuauucugca gagcaucuua aaaggaaagu ccacccuguc agacgcccag      960

guggagagau acgacgaaca caaaaaggau cucgagaucc uuaaaaaggu cauuaagaaa     1020

uaugacgagg augguaagcu guucaaacag guguuuaagg aagacaacgg caacggguac     1080

gugaguuaua ucggauacua cuugaauaaa aacaagaaaa uuacggcuaa gaagaaaaua     1140

agcaauaucg aguucacuaa guaugucaaa ggcauucuag aaaagcaaug cgauugugag     1200

gacgaggaug uuaaauaccu gcucgguaag aucgaacagg agaacuuucu gcuuaagcag     1260

aucucuucca uuaauucagu gauaccacau caaauccacc uguucgaauu ggacaaaauu     1320

cucgagaacc uggccaagua uuacccgagc uuuaauaaca aaaaggaaga guucaccaaa     1380

aucgagaaga uuaggaagac auuuacuuuc cggauccccu auuacguagg gccuuuaaau     1440

gauuaucaca aaaacaaugg aggcaacgcg uggauauucc guaauaaggg cgagaaaauc     1500

cgcccaugga acuuugaaaa gauuguggac cugcauaaaa gugaagagga guucaucaag     1560

agaaugcuaa aucagugcac cuaccucccc gaagagacag uccugccuaa gucuuccauu     1620

cuuuacagcg aauauauggu guugaacgag cugaacaauc ucaggaucaa cgggaaacca     1680

auagauaccg acguuaagcu gaaacuuauu gaggaacugu uuaagaaaaa gacgaaagug     1740

acuuugaagu cgauccggga uuacaugauu cgaaauaacu ucgcagacaa ggaagacuuu     1800

gauaauucug agaaaaaccu cgagaucguc uccaauauga agucauauau agacuucaac     1860

aauauccugg gagauaaauu ugaauuagag augguggaag accugauuga gaagaucaca     1920

auucacaccg guaacaaaaa gcuacucaag aaauacaucg aggaaacuua ucccgaucug     1980

aguagcuccc agauacagaa gauuaucaau cuuaaauaca aggacugggg ccgcuugucu     2040

agaaaacugc ucgaugggau cacaggaacc aacauugaga cgggcgaaau cgacaccgua     2100

auuaauuucc ugaggaacag cucagauaau uuaaugcaaa uaaucgguag ucagaacuau     2160

uccuuuaaca augacauuga uaagcugcgg aagaaauaca ucccucagga gauuagcuac     2220

gaaguugugg agaaccuuua ugucucucca ucagugaaga aaaugaucug gcaggucaua     2280

cgcaucacag aggaaauuac uaaggugaug ggguacgacc cggauaaaau cuucauugag     2340

auggccaaga gugaagaggg caagaaaacc acaaucagca gaaagaauaa auugcucgcu     2400

cuguauaagg ccauuaaaaa ggacgaaagg gacucccaau acgagaagcu acugacugga     2460

cucaacaaac uggaugacuc ugaucuucgu ucgcggaagu uguaucugua cuauacccag     2520

augggccgag acauguacac aggggagaaa auagauuuag acaagcucuu ugauagcacc     2580

cauuacgaca aagaucacau caucccccag uccaugaaga aagacgauuc aauuauaaau     2640

aaccugguuc uugugaauaa gaacgcaaau cagacuacga agggaaacau cuauccugua     2700

ccaaguucua uuagaaauaa ccccaaaauc uacaauuauu ggaaguaccu gauggaaaaa     2760

gaguucauua gcaaggaaaa auauaacagg uugauccgca auacaccucu caccaacgag     2820

gaacugggug gcuuuauaaa ccggcagcua gucgagacua gacaauccac caaggcuauc     2880

aaggagcugu ucgaaaaauu cuacaagaaa ucaaagauua ucccagugaa agccauucuc     2940

gcgagcgacc ugaggaagga caugaauaca cuuaagucuc gcgagguuaa cgauuugcac     3000

caugcccacg acgcauuucu gaauaucgug gcuggggaug ucuggaaccg agaguucacc     3060

uccaauccca uaaacuaugu gaaagaaaau cgggagggag acaagguaaa auacaguuua     3120

agcaaguacu uuacucgucc gagaaaaucu aagggcaagg ugauuuggac accugaaaaa     3180

gguaggaagc ucaucguuga uacgcugaac aaacccuccg uccuuaucuc aaaugagagu     3240

caugugaaga aaggggaacu guucaacgcc accauugcug gcaagaaaga cuauaagaag     3300

ggaaaaaucu accuaccacu caagaaagau gaccgccugc aggaugugag caaguauggc     3360

ggguacaaag ccauaaaugg agcauuuuuc uuuuuggucg agcacacuaa gucgaagaaa     3420

cggauuagau ccaucgaacu guucccuuua caucuccugu cuaaguuuua ugaggacaaa     3480

aacacaguuc uugauuacgc cauuaaugug cugcaguugc aagaccccaa gaucauaauu     3540

gauaaaauca acuauaggac cgagaucauu aucgacaauu ucagcuaccu cauuucaaca     3600

aagaguaacg acgguuccau aacugugaag ccaaaugaac agauguacug gcgaguagau     3660

gagaucucua accugaaaaa gauugaaaac aaauauaaga aagacgcuau ccuaaccgag     3720

gaagaucgca agaucaugga gagcuacauu gacaagauau aucagcaauu uaaagcgggc     3780

aaguacaaaa aucggagaac gaccgauaca aucauugaga aguaugaaau cauugaccug     3840

gauacucuug acaacaaaca gcucuaccag cuguuggucg cauucaucuc ccugucauau     3900

aagaccagca auaacgccgu ggauuucaca guuauagggu uaggaacuga guguggcaaa     3960

cccaggauca ccaaucuccc ugacaacacg uaccuggugu acaagaguau uacagguauc     4020

uaugaaaagc guauucgcau caaauga                                         4047


<210>  3016
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3016
augaaaagcg agaaaaagua cuauaucggc cuggacgugg ggaccaacuc cgucggaugg       60

gccguuacag augaguucua caauauucuc agagcuaaag guaaggaccu uuggggcgug      120

aggcuguuug agaaagcaga uacugccgcg aacacgcgga uauuccgcuc ugggcgacgu      180

agaaaugaca ggaagggaau gcgguugcag aucuuacgcg aaauuuuuga ggaugaaauc      240

aagaaaguag acaaggauuu cuaugacaga cuggaugagu caaaauuuug ggcugaggac      300

aagaaaguga guggcaagua cagccuauuc aacgauaaga auuuuuccga caaacaauau      360

uucgaaaagu uucccaccau uuuccaccuc aggaaauacc ugauggagga acaugguaag      420

gucgauaucc gguauuacuu ucuugccaua aaccagauga ugaaacgccg agggcacuuc      480

cugaucgacg gacagauuuc ucaugugaca gacgauaagc cuuugaaaga gcagcucauc      540

cugcugauua augaccucuu aaagaucgag cuggaagagg aacuuaugga uucgauauuu      600

gagauuuugg cagacguuaa cgaaaagaga acugauaaaa agaauaaccu gaaagagcua      660

aucaagggcc aagacuucaa uaaacaggag ggcaacauuc ucaauucaau cuucgaaagc      720

aucgugaccg ggaaggccaa gauuaaaaac auaaucagug augaggacau ucuggaaaag      780

aucaaagagg auaauaagga agacuuuguc cuuacaggag auuccuacga ggagaaccug      840

caguauuucg aagagguguu gcaggaaaac auaacccucu uuaauacucu gaaaucuaca      900

uacgacuucu uaauucugca aagcauccuc aaggguaagu ccacccuuuc agacgcucag      960

guagagaggu augaugaaca caaaaaggac cuggagaucu ugaaaaaggu uauuaaaaag     1020

uacgaugagg acggcaagcu auuuaaacag guguucaagg aagauaacgg gaauggauau     1080

gucaguuaca ucggcuauua ccugaacaaa aauaagaaaa uuacggcaaa gaaaaagaua     1140

agcaacaucg aguuuacuaa guacgugaaa gguauucucg aaaagcagug cgacugugag     1200

gaugaggacg ugaaauaucu gcuugggaag aucgaacaag agaauuuccu guugaaacag     1260

aucucuucca uuaacucagu cauaccacau cagauccacc ucuuugaacu ggauaagauu     1320

uuagagaauc uggccaagaa cuacccgagc uucaauaaca aaaaggaaga guuuaccaaa     1380

aucgagaaga uucggaaaac auucacuuuu cguauccccu auuacguugg accucuaaau     1440

gacuaucaca agaacaaugg cggcaacgcu uggauauucc gcaacaaggg ggaaaagauc     1500

agaccaugga auuucgagaa gauuguggau cuccauaaaa gugaagagga auuuaucaag     1560

aggaugcuga accaaugcac cuaccuuccc gaggagacag uauugccuaa aucuuccauu     1620

cuguauagcg aauacauggu gcucaaugag cugaacaauc uucggaucaa cggaaagcca     1680

cuggacaccg augucaaguu gaaacucaua gaagaacugu ucaagaaaaa gacgaaagug     1740

acuuuaaagu cgauucgaga cuacaugguu cgcaauaacu uugccgacaa agaagauuuc     1800

gacaauucug agaagaaccu ggagaucgcg uccaauauga agucauauau ugauuuuaac     1860

aauauccuag aagacaaauu cgauguggag auggucgaag accucauaga gaagaucaca     1920

auucacaccg guaacaaaaa gcugcuuaaa aaguacaucg aggaaacuua ucccgauuug     1980

aguagcuccc agauucagaa gaucauaaau cugaaauaca aggacugggg cagacucucu     2040

aggaaacugu uagaugggau uaagggaaca aagaaggaga ccgaaaagac ggacaccgug     2100

aucaacuuuc ugcggaacag cucagauaau cuuaugcaga ucauuggcag ucaaaacuau     2160

uccuucaaug aguacaucga caaauugcgc aagaaauaua uuccucagga aauaagcuac     2220

gagguaguug agaaccucua cgugucucca ucagucaaga aaaugaucug gcaagugauu     2280

agagucacag aagagaucac uaaggugaug gguuaugauc cggacaagau uuuuaucgaa     2340

auggcaaaaa gugaggaaga gaagaaaacc acaauaagca ggaagaauaa acugcuagac     2400

cuguacaagg ccaucaagaa agaugagcgu gacucccagu augaaaagcu ccugacuggg     2460

cuuaacaaau uggaugacuc ugaucugcgg ucgcgaaagu uauaccucua uuacacccag     2520

augggcagag acauguauac aggagagaaa auugaucugg acaaacuuuu cgauagcacc     2580

cauuacgaca aagaucacau cauuccccag uccaugaaga aggacgacuc aaucauaaau     2640

aaccugguuu uggugaauaa aaacgcuaau cagacuacga agggcaacau uuacccugua     2700

ccaaguucua ucaggaauaa ccccaaaauc uauaauuacu ggaaguaucu cauggaaaaa     2760

gaguuuauua gcaaggaaaa guacaaccgc cugauacgga acacaccucu aaccaaugag     2820

gagcucgggg gauucaucaa cagacagcug gucgaaacua ggcaauccac caaagccauu     2880

aaggagcugu uugaaaaauu cuaucagaag ucaaaaauca uuccagugaa ggcaagccuu     2940

gcuucugauu ugcgcaagga caugaauaca cugaaauccc gagagguuaa cgauuuacac     3000

caugcccacg acgcguuccu caauaucgug gccggugaug ucuggaaccg ggaguuuacc     3060

aguaauccca uaaacuacgu gaaggaaaau cgugagggcg acaaaguaaa guauagccug     3120

ucuaaagauu ucacuagacc gaggaagucc aaggggaaag ugaucuggac accugaaaag     3180

ggacgcaaac uuauuguuga cacgcugaac aagcccucag uccuaaucag uaaugagagc     3240

caugugaaaa agggcgaacu cuuuaacgca accauugcug guaaaaagga uuacaagaaa     3300

gggaagaucu accugccauu gaaaaaggac gaucggcugc aggacguguc gaaauauggc     3360

ggauacaagg ccauaaaugg cgcuuucuuu uucuuagucg agcacacuaa guccaaaaag     3420

agaauuaggu cuaucgagcu cuuuccucug caucuucuga gcaaauucua ugaagauaag     3480

aacacaguuu uggacuacgc caucaaugug cuccagcugc aagaccccaa aauuaucaua     3540

gauaagauua acuaucgaac cgagaucauu aucgacaauu uuucauaccu aauaaguaca     3600

aaguccaacg augggucuau uacugugaaa ccaaacgaac agauguauug gcgcguagac     3660

gagaucagca aucugaagaa aaucgaaaac aaguacaaaa aggaugcaau ucuuaccgag     3720

gaggaccgga agaucaugga auccuacauu gauaaaauau aucagcaauu caaggccgga     3780

aaauacaaga auagaaggac gaccgacaca aucauugaga aauaugaaau caucgaucuc     3840

gacacucugg auaacaagca guuguaccag cuguuagucg cuuuuauuuc acucagcuau     3900

aaaaccagua auaacgcggu ggacuucaca guuauagguc ugggcacuga gugugggaag     3960

ccccguauca ccaaucuucc ugauaacacg uaccuggugu auaagucuau uacaggaauc     4020

uacgaaaaac gcauucggau caaguga                                         4047


<210>  3017
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3017
auggagaaga acuacuaucu gggccucgac cuugggagca auuccguggg augggccgcu       60

accgaugaag acuacaacuu ccugagauug aaagguaaga cagcaugggg cgccaggauc      120

uuuucugagg cgaaagauug caaggaacgg cgcacuuauc gaucaaauaa acguagauua      180

gcuaggcgga aguaccgccu gguccuacuc aagcagcugu ucgccgagga aaugacgaaa      240

guugacaaga ccuuuuuccu uagacuggca aacaguacau auugggagga ggauaaaacu      300

aaggacagcg augggaauga aauuggcaaa aacuugauau uuaagaccca cgaggaagag      360

aagcaauacu auacaaaaua ccccaccauc uggcaucuca ggaaggcccu gauugagaau      420

gacgaagagg cucuguccaa ccuccgguac guguauuuag caauccacca uauuguaaaa      480

uaccgcggaa auuuccugac ugaaggugag auggggaacg auaucccucc aauaacaacc      540

aucgaagaga uuaaucagac gcuuucuuug cuggaggugg acucgucaga agaugccacu      600

gacaagacca cauauaucaa accggagagc uacaacgaac uauuuacuau ucucgagaau      660

aacaaucuga acaaggucaa aaagcagcag gaaaucgaua aacuuuucau aaguuccaaa      720

gagcgaucug agaacauuaa gaauuauauc aaccuguuua aaaccauuau cgugggaggc      780

agcuucuccu ugaagaaaau caaugaagac acagaggaca uuucaguuga uuuuaccaag      840

gacuucgaaa acagugaggc ugauauaagc aaggcccucg gcgacucuuu uaaucuggug      900

uuaaucgcga aaaaguacua ugauuacauu auccugaaag aacuccuugg gaagaacaaa      960

gagauauccg aggcaauggu ccaaauuuac gaagaacaca aggacgaucu gaagucauug     1020

aaaacgaucc uaaaggaccu ggauaauaaa cucggaaacg acgguaagga uagaaucuau     1080

uacagcgugu ucaaacagga acccacugag aaguucaagg agacaaauua ucugaguuuu     1140

guacagggcg ggaaacuuga ggagcugaac aaguacauua aaaagaucuu ggaaaauaac     1200

gagaaagaca uugacgauaa ggaaaccuau aaugcccucu acgagaaggc uuguaaggga     1260

gaccuguuca agaaacaggc caacgcaugc acuucuuuaa uaccucauca auuucaccug     1320

agggagcuaa agaaaauccu cgaaaaugcu ggccggaagu auuccuuccu gaacgauauu     1380

agcgacaaaa ucaucaagau uuuuaaguuc cguguuccau acuacuaugg uccccuuaac     1440

aaugagucga acgcccacuc uugggugguc aaaaaggaag ggcaggagaa agcgaagaua     1500

acaccuugga auaucaacga aauuaucgau accccaaaaa cgcgcgacaa cuuuuucaag     1560

aaguugacca auuccuguac auaccuggug ggagagacug ugcuccccaa aaccucacug     1620

uauuacgaga aguaucuugu ccuggauaga uugaauaaaa ugcagguuaa cggcgacuac     1680

cucagugcca gcgaaaagaa ugaugugcug aaauuuauuu uauccaggaa gacaacuacc     1740

aucgagcaga uacggacaua ucugucuaag aacaaaagca ccaaggcaaa ugacaucuca     1800

auuaguaaca ucaaagcuac ugauccuuuc aaugccguau cccaugcuuu uuacggcaag     1860

cacccagacc uaaacauuaa ucucugcgag gaacugauca aauucgccac gauauacgca     1920

gaugacaaga aagaacuuaa gaaguuugug agcucuaacu ucaacuugac agacgaguuc     1980

auuaaagagu aucugaauac cccgacuaac aagugggggc gacucucacg caaauuucug     2040

uguggaggug aucaauuaga aauguacuau gucgacgaua auggcauccu gcauaguauu     2100

cuuaacaucu uggaggaaac caaugagaac cuccaggaga uacugaauaa cgaaaaguac     2160

ggguucaauc agaaaaucgc cgacuuuaac aauacacuaa ccgcugauaa gacuacagag     2220

gaacaaauug aggacauccu ggauagcacg cccgcgcuca ugagaagguc cauuauccag     2280

acccuggcag ugguugacga ugugauaaag gccgcuggaa aagccccuca gaagauuaug     2340

auggaaguca cucggacaga cggcaaaaag gaggagacca aaucucgcua ugugcagcuu     2400

aagaagcaaa ucacaaacga uaaaguauug acuucggacg aaaagaaaga gcuguuaacc     2460

aaucucgaaa acaagaaaau ccagcugaag gguaagcaca uuuaccuuua uuucaugcag     2520

auggggaucg auuuuuacac gggcaaaauu auggacauaa augacguuau caacggaaag     2580

uacgauauug accacaucau uccacagagc augaucaaag augacucccu ggauaacaug     2640

guguuggucu caaaggacua uaaucaaaaa gugaagaagg auauauaccc caucugcgac     2700

gagauuagaa acaauaugcu cgauaaaugg aagggcuauu acgcaaggaa aaucauuagu     2760

gaagagaagu auaaccgucu gaaacggacc aaggagcuaa augccgacga aaucaacgcu     2820

uucauaaauc gacagauuaa cgucgugaau uacucuaaca uccagaucaa aagaauuuuu     2880

gagauaaagu auccugaaac aaagaucauu uucagcaaag ccuccuuucc aucacugcuc     2940

aggaaggagu acgaaaucgc aaaaaugcgc gaucugaaug acgcgcauca cgcuguugac     3000

gccuaccuua acauugugug ugggaauauc uugacuaccc uguauacaga uauauucuac     3060

auguuuaagg agaaaaagga ggaccauaag aacaucagcu ucaauaugga aaaacgguua     3120

auuaacuaua ucgauaagaa uaaccucaaa gacaagauua aaacuucuug ccugagacac     3180

gaugcacuua ucaccuacaa gcccgacuau gauuccggaa aguuuuacaa agagacgaua     3240

uucaagaaaa guggugacaa ggaaacaggc cugauuccgc uacauaccaa agggccuaug     3300

agcgauacua agaaguaugg aggcuacucu cagcucuccc aaucauacau caucgccgua     3360

gaguauaaac ugaagaacaa aacagucaag aaauugugua gggugcccac ccuguacuua     3420

aagcaguaua aaaaugaccu caacacuauu gcuaagauca ugauagauga cgagaaggcc     3480

accgauauua aaguucugcg caagaucuac cuuaaucaga aaauucgaua uaacgguugc     3540

uacuaucuga ucuacacaag uaaggaaaaa cagaauaagu acaagauggc auaucaaaac     3600

uacuuggaca augacuaucu cauauaccug aacaaauguc uaaagaaaau ugaugagcug     3660

gacgaugggg aacuuacgag ccaggaguuc aucgugaaua aggacggcga uaaauuucuc     3720

aucgacaaga accacaaucu ggaaauuuuc aagucgauca aagagcagua uaacaagacc     3780

auuuacgauu auacuccaua cauaguccaa ucccgggaga ugaaagaaga ggacuuuaau     3840

aacuugucuc ugaagaaaca gaucgauaca uuacucaaua ugauuaagau ccugagccgu     3900

ucaaacgaac aggcuaaguu cgagaccgcc uuuuucgaaa acuuuacaaa aguguuccuu     3960

aagacuauga ccaucacgaa ugagaaagua uacauuguga acgagagucc uuccggacug     4020

uuuaagggca aagaagaguu gauauga                                         4047


<210>  3018
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3018
augaagaaau ucgaggacua cuaucugggc cucgaucuug ggaccagcuc caucggaugg       60

gccgugacaa acgaaaagua cgacauucug agauuuaaua aaaaguucau gugggguacu      120

cacuuguuug auaugggcaa cacggcuucu gagaggcgga uauuccgcac cucacgacgu      180

agauuaaaaa ggaagaagaa ucggaucgaa cugcuacaga cacucuuuag ugcagagaug      240

agcaaaaagg acuucggguu uuuccaacgc cugaaagaau cccaguauuu uauugaggau      300

aagacuuuca aaaacaagaa uacccuuuuu auggacgaga acuacacaga ucaggacuau      360

cauauggaau accccaccau cuaucaccug agaaaggccu ugauugauaa ugaggaaucu      420

gcguucgaga ucaggaaacu cuaccuggcu auaucgcaua uccugucaca ccggggacau      480

uuucucuucg agggcgaauu aagcgacaac cuggauuucg acgauauuuu uaaugaguuc      540

gucaaguacc uuaacgaaga guugaauauc gacuuuaaaa uuaagaguga cguuucccug      600

caggccauca aagaaauacu aacugauaag augcucaacc gcaccaaaaa gaaggagaaa      660

cugucugagc uucuggaaga ggaaaagaau aaaauuuauc aagagaucug gaaguugcuc      720

guggguaacg aagcaaaaau gagcacaauu uucgacgaua aggaguacga ggaagccaag      780

aucgcuuuuu ccaaagggaa uuaugacgag aaggaagagg aauacgaguc aauccuggga      840

gagaaaauuu uauucguaca gaaggcaaaa gccauauaug auuggaugcu gcuccagaac      900

auccuuaagg aagacaagua ccugaguuau agcaaaguga aguuguacaa cgagcacaaa      960

aaggaucuaa aaauucugaa ggacguccuc aagaaucugg auaaaaagau cuacaaagaa     1020

auauuuucug agaacggccu uuaugaggaa uaccugcgaa uugagaagaa aaagaucggc     1080

uccaaagaag agaagacgaa ggacaucuug uauaauagac ucaaaaagau uuucgaaaac     1140

agggauaaua uggcuaucga ggacauucug aacaaaauag aguuagacuc auuucugccu     1200

aagcagaaaa ucaaggauaa uggggugauu ccacggcaag uucaccagaa ggaacuagag     1260

aucauccuca aaaacgccga aaaguacuuc aaauggcuga uugagaagga cgaaacugga     1320

cuuagcguga gugagaaaau aaugaagauc uuuaaguuca aaauuccgua uuacgucggu     1380

ccccuuaauu cuaagucccc uuuugcgugg aucgagcgua aagaauaugg caagauucug     1440

ccauggaacu ucgagaauau gguggauaug agcaaaaccg cagaaaaguu caucaagcgc     1500

augacaaaca aaugcacuua ccuccccgag aaggacguaa uaccuaaaaa uucgcugcuu     1560

uacaccaagu auaugguucu gaacgaauug aauaaccuca aaaucagagg ggagaagauu     1620

aaaguggagc ugaagcagaa gaucuacaca gaauuauuuc agaaaaacaa gaaaauugga     1680

augaagaccc ugaucgaguu ccuaaaaucu caaaauauag ucgugacgaa gguggaaauu     1740

acuggcaucg augguacauu uaacaccucc cucgggucau auauugacau gaagaaaauc     1800

uucggagagg agaagauagu caaagauagu uacaagaaaa ugaucgaaga cauuauccug     1860

uggauuacuc uuuauggcga ggaaaagaag uugaucaaaa gcaagauaga ggaaaaauac     1920

ccagagaagg uuacacccga ggaaaugaaa cugauucuca agcugaagua uagggauugg     1980

ucccgguuau cuaaagaguu ucugaccgaa aucgagacga uggacgaaga gaccggcgag     2040

cuuaucagca uuaucgaaau guuguggcga aagaaugaga acauuaugga acuccuguca     2100

uacaaauacg auuucaguaa ucgcauaaag gaguauaacg ggaucaauga aaaagagaag     2160

aacauuacau acgaggaccu agugaaggau cugcagaucu ccccuagcgu gaaaaagaug     2220

auuuggagag cccucguaau cgucaaagaa auaaagaaag ugacuaauaa gucuccgaaa     2280

aagguuuuua ucgagauggc uagggacacc cagacagaaa agaaacggac ugagaagcgc     2340

aaagacaagc ugcuugaguu guauaaaaac auuaagaaug augacucaau caaggaacug     2400

gagaaaaacu uaaaggauaa aaccaaugac gaucucaaga guaaaaagcu guaccuuuau     2460

uaccugcaau ugggaagaug uauguauagc ggugaaguga uuaggcucga ggaccugaug     2520

aacgauaauc uauacgacau cgaucauaua uacccacagu ccaagauuaa agacgauucu     2580

uucgacaaca caguccuggu gaagaaacag aucaacgccg auaagaccaa ugaauauccc     2640

aucgacaaau cgauucagaa caagugcaag gcauuuugga cuagccucaa agagcguggc     2700

cugaugucca auacgaagua cgagcggcuu acacgaaccg aaggguucuc agacgaugag     2760

uuggcuggau uuauaaguag acaaaucgua gaaacuaggc aggccaccaa aguucuggcg     2820

gacuuacuca aggugauuuu cggcucugau acacgcaucg ucuaugugaa agccggucug     2880

accagcgacu uucggcagaa guucgggauu uacaaauuca gagagcuuaa cgauuaucac     2940

caugcacacg acggcuaccu gaauaucguc acuggaaaca uauauaauac acuauuuacg     3000

gauaacccuu acaauuucau caaggacagg aagaaagaua accgcaccua caaucucaac     3060

aagguguuug aacgagagaa ugacguuugg aacccagaug ugaugcuguc caaaauugag     3120

cgguauaucu acaagauucg ucccuuguuc acuagagcuu cauaugaaca gaaaggcggg     3180

uuuagcgacc aaaauaucuc uuccgagaag gacaagcuga ggccuuuaaa aaguagcgga     3240

guacucucua acauaaagua cgguggcuau gauaaaauua agaacuccua cuucuuuauc     3300

gucgaauaua cagagaaaaa gaagcgcgug cggucaaucg aaacccugcc aauuuacauc     3360

aaaaagagua uaagcacaaa agaggaccuu gagaaguacu guaaagaaga acugaaguug     3420

caggaaccca agguucucgu gucgaaacug aauuauaagu cccuaauuua cucuaacggg     3480

cacgaguauu acaucaauag caaaacugcc gauucaauua ccauccagaa cggaauacag     3540

auuauccuga cgaaggagaa uuaugaaacc uacagaaaaa ucuauaagaa ccuugaguac     3600

cucaaacuga uuuacaagga cgaaaagauc agggaggaga aaauuuugga gaagauaccg     3660

aaaauccuga agaauaacaa aaaugaucga gacuuaagug aggaugaaau uuccgagaag     3720

uucuggaagc ucuauaaaga aaucuuugag aagaucaaaa uuggcauauu cucuaaguuu     3780

aaaagcacac ugccuaucua cauugaagac ugcaagaagu ugaaagauuu cacuacccug     3840

aacaauuaug cuaagucccg cuucauccaa gagauuuuga aaugucucaa cucacagcag     3900

ggugaguuug acacaaagua cacucauaau uauagcaaaa aguacaacac caagcugagu     3960

aaaucuaaga uccuagaaaa ugauuucuau auaaucaacc agucccccac ggggcuguuu     4020

gagaaaaagg ucuuccuuga caaauga                                         4047


<210>  3019
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3019
augggcgagg acgggaagcc ccugagagug aaaggaaaga ccgccaucgg ugcuaggcuu       60

uuccaggaag gcaacccugc agccgaucgg cgcauguuuc gaacaacucg uagaaggcuu      120

aaucggcgca aauggagacu gaaguuguua gacgagauuu ucgauccaua cauaacgccg      180

gucgacagca ccuuuuucgc gaggcugaaa caauccaacc uaucucccaa ggauucacgg      240

aaggaauuua aagggaguau guuauucccu gaccugacag auaagcagua ucaccuugac      300

uacccaacua ucuaucaucu gcgccacgcu uugaugaccc aggauaaaca guuugacauu      360

cgaaugguuu accucgccau ccaucacauu gugaaguaua gaggaaauuu ccugaacagc      420

acacccguag auuccuuuaa agcaucuaag gugaauuucg acgaucaauu uaagaaacug      480

aacgagcucu acaccgccau caauccugaa gagucguucc agauaaacuu agcuaauuca      540

gagaagaucg gccagcaguu ucuggaccca agcauuagga aauucgacaa gaaaaagcaa      600

auccccaaaa uugucccugu gacuauggau gacaaggcag uugauaagcu caacgguaaa      660

aucgccagug aaauaauuaa ugcuaucuug ggguauaagu ccaaacugga cguggccguc      720

cagugcgauc cagaggacuc uaacccgugg gcggugaagu ucgaugacga agauauugac      780

gcaaaacuac agaagauccu ccccgagaug gaugagaauc agcaaagcau cauuggaaua      840

auccaggacc uguacuccca gguaacacuu aaccaaauug uuccuaaugg caugucacug      900

agugaaagca ugaucaagaa auacaacgac caucacgauc acuugaagcu cuauaaaaag      960

cugauaggcc agauuucuga cuccaaaaag aagaccgccc ucaaaaaggc uuacgaucag     1020

uaugugggga acgacggaaa agucaucgag caggaugacu uuuacucaag cgugaagaaa     1080

aaucuggaug acagugaacu cucuaagcaa aucauggauc uuauugacgc cgauaaguuc     1140

augccaaaac agcggacguc ccagaacggu gugauccccc aucagcugca ccaacgugag     1200

uuggacgaaa uuauagagca ucagagcaag uauuacccuu ggcuagcaga gucgaaucca     1260

aacaaacacg aucugcaccu cgcuaaguau aaaaucgaag aacuggucgc cuuucgcauu     1320

cccuacuacg uuggcccgau gaucacuccu gaagaccaga agaaaucugg ggcggccgug     1380

uucuccugga ugaagagaaa ggagcccgga cagaucaccc cauggaauuu ugacgaaaaa     1440

guagauagga uggagucagc aaacaaguuc auuaaacgga ugacaacuaa ggacaccuau     1500

cuuauaggcg aggaugugcu gccugacgaa aguuugcucu acgagaaauu uaagguccug     1560

aaugaauuaa acauggugcg aguuaauggu gauagccuga agguggcuga caaacaagcc     1620

aucuaucagg aucuauucga gaacuacaag cauauuuccg ucaaaaagcu ccagaauuau     1680

aucaaaggga agacaggacu gcccaacaau ccagaaauuu cuggccuuag cgaccccgau     1740

caguuuaaca auucauuggg caccuacaac gacuucaaga aacuguuugg ggauaaggug     1800

gacgauccug accuccaaaa cgacuucgag aaaaucguag aguggaguac gguuuuugaa     1860

gauaagaaaa uacugcgcgc uaaacuuaau gagaucacuu ggcugacaga caagcagaag     1920

gaugacuugg aauccagcag auaucaggga ugggguaggc ucucuaagaa acuguuaacc     1980

ggcauuguga acgaucaagg ggagcggauc auugacgagc uguggaauac uaacaagaau     2040

uucaugcaga uccagucaga ugacgauuuc gccaaacgca uacacgaagc aaacgccgac     2100

cagaugaaga guacaaauau ggaggauguc cuagcugacg cguacaccag cccacaaaac     2160

aaaaaggcaa uuagacaggu ggucaaggug guugacgaua uccagaaagc caugggaggc     2220

guggcuccga aguuuauuuc caucgaguuc acgaggucug aggaccguaa uccccggcga     2280

accauaucga gacagaggca acucgaaaac acacugaaag auacugccaa gagccuugua     2340

aaauccauga auccuaacau cuugucagag cuggacaaug caagucgcuc uaagaagggu     2400

cucaccgauc ggcuguacuu auauuuuaca cagcugggga aagacaugua cacuggcgag     2460

ccaauuaaca ucgaugaaau uagcaauuau gacaucgauc auauacuucc ccaggccuuc     2520

auuaaggacg auuccuuguc aaacagaguc cucgugagca aagcuguuaa caauggaaag     2580

ucugccaacg ugccucugaa acuauuuggc gcaaagaugg ggcacuucug gaagcagcug     2640

gcggaggcug gacucaucaa uaaacauaag cugaaaaacc uucaaaccaa uccagacaca     2700

aucuccaagu uugccaugca cgguuucauu aggcgccagu uggucgaaac cagucaggug     2760

aucaaacugg uagcaaacau uuuaggcgau aaguaccgaa augacgacac uaagauaauc     2820

gagauuacgg cccggaugaa ccaccaaaug cgugaugaau uuggguucau caaaaauaga     2880

gagauuaacg acuaucauca cgcuuuugau gccuaccuca cagcauuccu gggaagguau     2940

cuuuaccauc gcuacaucaa gcugcggccc uauuuugugu acggcgacuu caaaaaguuc     3000

agagaggaua aaguuaccau gaggaauuuu aacuucuugc acgaccucac ugaugacacc     3060

caggcucgaa uagccgauac agaaaccggu gaggucaucu gggaccgcga aacuagcauu     3120

aagcagcugc gggaugugua ucacuacaag uuuaugcuaa ucucucagga gguguauaca     3180

cugagagggg cgauguucaa ucaaacgauu uacccggccu cccaggcugg caggaaaaag     3240

cucaucccua ugaaagcaga caagccaguc gauguuuaug gaggcuacuc agggaguacc     3300

gacgccuaca uggcuauagu gcguauucau gacaaaaagg gagauaaaua uaagguggua     3360

ggugucccca ugcgcgcccu gaaccggcuu aauacugcaa agaacgugag cgacgcgaaa     3420

uacgaucagg aauugaagga gguucugaca ccucaguuua ccaaaucgaa gaaaaacaga     3480

aagacaggcg agaucacuca aaccguggaa gacuucgagg ucguguuagg gaagguuaug     3540

uauaggcagc ucgugaucga uggagacaaa aaguuuaugc ugggcuccuc uacguaccag     3600

uauaaugcca aacagcuugu acugagcgau gaaucaauua agacccuagc uagugagggu     3660

aaacucaacg aggcccaaga auccgcagcu uacgacaagg ucuaugauga gauacuggac     3720

aaggugaaua aauacuucgc cuuguacgau acaaacaagu uucgacagaa acugucuuua     3780

gggaaggaca aauucguggg ccuccccaau cacaacgucu uugacggaag caagaagauc     3840

uccucaggca aacgggaaau ucugaaugag auccuuaacg ggcugcaugc aaaugccacu     3900

uugggaaccc ucagcgauau uggcuucagu acaccauucg guaagaugca gcagccucgc     3960

gggaucggac ugucuuccaa cacuagcaua gaauaugagu cguuuaccgg ccuauucaaa     4020

agaacgaucu uucugaagga ccuuuga                                         4047


<210>  3020
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3020
augaagaaac ccuacaccau cgcccuggac auuggcacaa acagcguggg gugggucguu       60

gugacugaug acuauagagu accuacgaag aaaaugaagg ugcucggaaa uaccgagagg      120

aaaacaauaa agaagaaccu uaucggugcu cuguuguucg auuccggcga cacugcagaa      180

gggacccggu uaaaacgcac agcccgacca cguuacacca gaaggaagaa ucggcugcgc      240

uuucuaaaag agauuuucac ugaagagaug gcgaaggucg augacggauu uuuccagaga      300

cucgaagauu cuuuuuaugu gcuggaggac aaagagggca acaagcaccc gaucuucgcu      360

aaucuugccg augaaguugc auaccauaag aaauauccca caauuuacca ccugaggaag      420

gaguuggugg acaacccuca aaaagccgau cuccggcuga ucuaucuggc ugucgcacau      480

auaaucaagu uucgcgguca cuuccucauu gaagggaccu uaucaaguaa aaauaacaau      540

cugcaaaaga gcuuugacca ucuuguggau acguacaacu ugcuguucga ggagcagcga      600

cuacucacug aaggaaucaa ugccaaagag cugcuuuccg cugcccuguc uaagucgaag      660

agauuggaaa accucauuuc acugauccca ggccagaaaa agaccggcau auuugggaau      720

auuaucgcgu uaagccuggg acucacaccc aacuucaaag caaauuuugg ucuuaguaag      780

gacguaaaac ugcaguuggc caaggacacu uacgcugaug accuagauuc ccugcucgcc      840

cagauuggcg accaguaugc agaucuguuc cuugcugcca agaaccuguc ugacgcgauc      900

uugcucagcg auauccugac cgaguccgac gaaauuacaa gggccccuuu aucagcaagu      960

augguuaaac gguaccguga gcaccacaag gaucugguga cccuaaaaac gcucauaaag     1020

gaccagcugc cagagaaaua ucaagaaauc uuccuugaua agacuaagaa cggguacgcu     1080

ggauauauug agggccaggu cagccaggaa gaguuuuaca aauauuugaa gcccauccug     1140

gcccgccucg acgguucuga accucugcuu cugaaaauag acagagagga uuucuugagg     1200

aagcagcgga cauuugacaa uggguccauu ccacaucaaa uccaccucga ggaacugcau     1260

gcuaucuuac gacgccagga gguguucuac ccguuucuga aagauaacag aaagaagauu     1320

gaaucacuac ucaccuucag gauccccuac uaugugggac cucuggcccg gggccacagc     1380

cgcuuugcau gggucaaaag aaaguucgac ggcgccauua ggccauggaa uuuugaggaa     1440

auaguugaug aggaggcuag ugcgcagauc uucauugaga aaaugacuaa gaacgaccuu     1500

uacuugccca augaaaaagu gcugccuaag cacucucucc uguaugaaac auuuaccgua     1560

uacaacgagu uaacgaaagu gaaguaugca accgagggga ugacacgucc acaauuccug     1620

uccgccgauc agaagcaggc uaucgucgac cuuuuguuua aaacuaaucg gaaggugacc     1680

guuaaacagc ucaaggaaaa cuacuucaaa aagaucgagu gcugggauag cguggaaauu     1740

acaggagucg aggacucguu caaugccucu cuggguacuu aucaugaucu acugaagaua     1800

auccaagaca aagauuuucu cgacaacccc gacaaucaga agauuaucga agauauuauc     1860

cugacccuua cauuguucga ggacaaaaag augauaucca aacgacugga ucaguacgca     1920

cacuuauuug acaagguggu acucaacaag cuggagagac aucacuacac cggcuggggg     1980

aggcuuucag gaaaacugau caauggcauu cgcgauaagc agagugguaa aacuaucuug     2040

gacuuccuca aggccgaugg guuugcuaac cggaauuuca ugcaacugau ucaugacagc     2100

gaacuauccu uuaucgauga gauagccaaa gcacagguua uuggcaagac ggaauauucu     2160

aaggaccugg ugggaaaccu cgcgggcagc ccggcuauca aaaaggggau uucacagaca     2220

aucaaaauag ucgaugagcu ggugaagauc augggauacc uuccucagca aauugucauc     2280

gaaauggcca gagagaacca gaccacugca caggguauua aaaaugccag gcagcgcaug     2340

cgaaaguugg aggaaaccgc uaaaaagcug ggcaguaaca ucuuaaagga gcaccccgug     2400

gacaauuccc aacuccagaa cgauaaacgg uaucuguacu aucuucagaa ugggaaggac     2460

auguacacag gagacgaucu ggacauagau uaucuaagcu cuuacgacau ugaucacauc     2520

aucccacaau cauucauuaa aaacaauagu aucgacaaca agguucucac cagccagggc     2580

gccaaucgug guaaacugga uaacgugccu uccgaagcaa uuguaagaaa gaugaagggg     2640

uacuggcagu cuuugcugag ggcuggcgcc auaucgaaac agaaguuuga caauuuaacu     2700

aaagcggagc gcggaggccu cacacaaguc gauaaggccg gguucaucca gcugcagcuu     2760

guggaaacgc ggcagauuac caaacauguu gcucaaaucc uggacagcag auuuaacacu     2820

gaguucgaug accacaauaa gaggauucga aaggugcaua ucauaacauu gaaauccaag     2880

cucgucucag acuuucgcaa agaguucgga cuguauaaga uccgggauau uaaccacuac     2940

caccaugcac acgacgccua ucuaaaugcu gugguagcca aagcaauccu ggguaaguac     3000

ccccagcuug cgccagaguu cguguauggc gauuacccca aguauaacag uuuuaaagaa     3060

agacagaagg ccacccagaa aacacucuuc uacucuaaca uucugaaguu uuucaaagac     3120

caagaaagcc ugcauguuaa uuccgauggg gaggagaucu ggaacgcuaa uaagcacuug     3180

ccuauaauua agaacgucuu aucaauccca caggugaaua ucgugaaaaa gacugaaguc     3240

cagaccggag gcuuuuacaa agagagcauu cucucuaagg guaacuccga caaacugaua     3300

ccgaggaaga auaacuggga uacgcguaaa uaugggggcu ucgacagucc caccguugcc     3360

uacagcgugc uugugaucgc aaagauggaa aagggaaaag cuaagguacu gaaaccuguc     3420

aaggagaugg ugggcauuac aaucauggaa cgcauugccu uugaggagaa uccaguugug     3480

uucuuggaag caaaagggua ucgggagauc caagaacauc ucauaaucaa gcugcccaag     3540

uacucucuau uugagcugga aaacggaaga aggcgacuuc ucgccuccgc uucagagcug     3600

cagaaaggca augaguuguu cuuaccuguc gauuauauga cuuuucugua ccucgcggcc     3660

cacuaucacg aacugaccgg uaguagcgag gacgugcuuc ggaagaaaua cuucguugaa     3720

cgccaucugc acuacuucga ugacauuauc cagaugauua acgauuuugc agagagacau     3780

aucuuggcuu cguccaaucu agaaaagaua aaccacacau aucauaauaa cucugaccug     3840

ccagugaacg agagggccga gaauauuauc aacguauuca cuuuugucgc ccucggggcu     3900

cccgcagccu ucaaauuuuu cgacgcgacc aucgaucgga agcgauacac gagcacaaag     3960

gaagugcuga augcuacccu uauucaccag ucagugacug gauuauauga gacaagaauc     4020

gaccugaguc aacucggcga aaacuga                                         4047


<210>  3021
<211>  4050
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3021
augaagcaga aauucuacgu gggccuggac auggggaccg ccagcgucgg augggcuguu       60

acagaugaca aguauaacau cauuaaaaga cacgguaagg cacucugggg cgugaggcuu      120

uuuaaaacug ccaauacggc ggaggaacgg cgcauauucc gagcugcccg uagaaggacc      180

caacggcgca agcagagaau ggagcuguug caggaaaucu uuggggauga gauuuccaag      240

acagacaucg gauucuacca gaggaugaaa gaaucuaagu auugguuuga ggauaaacgg      300

gacauuaacg gcaagcaacc cgaguuaccu uacgcacugu ucguagauga cgguuuuacu      360

gauaaagacu aucaugaaca guucccaacc aucuuucacc uacgcaaugu gcucgucaac      420

gaguggacac agaaguacga uauacgacug guguaucuug ccauccauca cauuaucaag      480

cauagagggc acuuccuguu ugaaggaaag aagaugucag agguuaccga cuucggcagu      540

gcuuuugagg cauucugcaa aguggccagc gauaaugaca uugaaaucac ugucgacgag      600

gaaaaguuga aagauauaga ggaccuccug aaguccaggc agaaaucuaa gacagcuaag      660

aaacaagaac ugggcaagau uaucgccggg gcggauaaac agaagaaagc acucgccgcu      720

uuagugaccg gauguauggu aaagcugucg gagauuuucg ccgacaaguc acuugaugac      780

ggugagcgga acaaaaucag cuuugcagau aguaauuacg acgaauacau cgcuaacguu      840

gaggaaacgu uggccgagcg uuuccugcua auugcggccg gcaaggcacu cuaugauugg      900

gcugugcugu ccgacauacu ugaagggucu acuuacauca gcgaggccaa agucaagauu      960

uauaaaaagc acggagauga ccugcgcaag uugaaaggcc uccugaagac cagaccggac     1020

gaguacaaau uaaucuuugg ucugcccgaa aagcaggcua auuauuccgc cuacauaggg     1080

auggugaaaa agaacggaaa gaaagugccu auugauaaga aaugcacaaa ggcagacuuc     1140

uauucauuuc ucaaaaagac ucuuaauaaa aucaacuucg aggaugaaaa ggagagggaa     1200

aucgccgaga guauuaucaa ugagauugaa aacgagaccu uucugccaaa gcaggucaua     1260

agcgacaaug gcguuauccc cuaccaauug caugaagcug agcuagaucg gauucugaaa     1320

aacgcggcac ucuacuaucc uuuccugucu gagaaggacg augaaggcua cacaaacaug     1380

gacaaaauca aggccaucuu uaaauuccgc auuccauauu acguggggcc ccuuaauacc     1440

uaucacuccg auaagggagg uaacucaugg gcuguacgaa agcaggacgg caaaauauac     1500

ccuuggaauu uugagagcaa gaucgaugcc ggggaaagug caagaaaauu cauuggaagg     1560

augacgaaca aguguacuua ucugaucggc aaagacgugu ugccaaagga gucucuccug     1620

uacuccaagu uuauggucuu aaaugaacug aacaaucuac ggauugaugg ugagaaagug     1680

agcguugaga ucaagcaggc cguguacgaa aacgucuuca aaaaguauca acgcgugaca     1740

gggaaaaagg uaaaggacuu ccucauaaga ucgggcauca uuggaaggga gcaggaacug     1800

ucuggcuuug accagaauuu caaauccuca cuuaaggcuu accaugauau caaacagauu     1860

gccggggaca ucaacuugag ucaaaaucag aaggaggaua uaauuaaaga aaucaccguu     1920

uuugcagaca gccagaagau gcugaaaaag cgucucaaga uuaaauaucc ggagcugucc     1980

gauaaacagg ugacuaaacu ugcggcuaag aaauacacag gauggggucg gcugucucga     2040

gaguucuugg aagaggucga aagcgugaac aaggagaccg gcgaaguccu caauaucaua     2100

ucagcccugu gggagacgaa cgagaauuua augcaacugc uaaguaacaa guaugacuac     2160

cucuccgaaa ucaaaagacu gaacgaugag gcagaagaca agagcgaggu gguuaccuau     2220

gaugacgugc agaaacuuua cguaucuccc gccgucaaga ggccugugug gcagacauug     2280

aaaauuguug aggaaaucuc aaagauuaug gggaguaagc cagagcgcau cuuuguggaa     2340

auggcucggg agcccggaca gaaaggcgau gucaagguga gcagaaaauc caggcugcuc     2400

gaaaaguaca aauauugcaa gaaggaggcc ccugagauau acgaaaaucu gguaaaagag     2460

gacgauaaca aguuacgcuc ugacaaacug uuccuuuauu acacucaaau gggucgaugu     2520

auguauucga augaagugau ugacaucgag gauuuguuua ccaaccggua cgacaucgau     2580

cacauuuauc cacagagcaa gguuauggac gauucccucg acaaucgugu ccuggugaaa     2640

agagaacuaa acucaaauaa gacagaugug uuccccgucc cgaaggagua cauggacaaa     2700

gcaaagacuc ugugggauau gcucaaaaag aaagaccuga ucaguaagga gaaauaccac     2760

aggcuuaccc gccgggaugg guuuucugaa aacgaguuga gcggcuucau ugcuagacag     2820

cugguugaaa caaggcaguc caccaaggcc gugacucaau uacucacgaa ggcguauccu     2880

gagucagaaa uaguguacgc caaagcuaau gcaguaagcg aguuucgaca uaaguucgga     2940

uuuacaaaag uccgcucuau caacgacuau caccaugcca aggacgcuua ccugaauauu     3000

gugguuggca acaccuauua cgugaaauuc acuaaggaug ccgcaugguu cauccggaau     3060

aaccccggga gaaccuauaa ucuuaagaaa auguuugagc agggaacagu cuccaggggu     3120

ggcgaaaccg cgugggugag uggggaggac ggaacuauuu ucacaguuaa gcgugugaug     3180

ggcaaaaaca acauccuguu uacgcgcaag gccuacgaag uacggggugg guuguucgau     3240

cagcaaauaa ugaaaaaggg caagggacag aucccaguca aaaauaacag cgaggacgau     3300

agacucugcg cuaccgagaa guacggcgcc uauaauaaag caucuggggc uuacuuuaug     3360

cugguggaau ccgaggacaa gaaaggaaac aagauuagga cucuagaguu cgugccuguc     3420

uaucuggcca aggaacucga aaaaucagag gaugcagcca uccgauacug uguugagaag     3480

cuggaacuua aaaaucccga cgugcgggua cgcaagauua aaauggauag uuuguuuaac     3540

guggacggcu ucagaaugca ccugagcggu aggacaggga agcaguuaau cuuuaaaugc     3600

gcugagcagc ucguccugga ugaagagucg caaaagauac uuaagaaaac cgcgaaggug     3660

uucgccgacu auaaagaaaa uaagaacauu aaaaucuccg auaagaucga gcugacagac     3720

gagaugcuaa uuaagcucua cgaacuguuu uauaaaaagu ugaaagacac uguuuacaau     3780

auacggcugu cuacccaggu gaagacguua gagagcggaa aagaaaaguu cgagaagcuc     3840

ucacugcuug auaaaugucu ggucaucgaa gagauuuugc aucucuucca gugccagagu     3900

guggcagcua accuguccuc uaucggcggu gccgccaccg cuggcauucu aguucugaau     3960

aacgagauca gccgaugugg gaagguggaa auaaucaauc aauccauuac aggauuuuac     4020

gagcagguca ucgaccuuaa aaacauuuga                                      4050


<210>  3022
<211>  4050
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3022
augcagaagu acuaucuggg ccucgacauc gggaccaaca gcgugggaug ggccgucaca       60

gaugagaauu acaaccuuug caaauucggu ggcaagagaa ugugggggau uaggcuguuu      120

gacggagcuc aaacugcaga agaacggcgc augaaucgau ccaaccguag aagguugucu      180

cggcagaaac agcgcauaga uuuacugcag gaacuauucg ccaaggagau cgcgaaaguu      240

gaccccacgu uuuucauuag acucaaugaa ucaaggcugu auauggagga uaagaguacc      300

ggcacaaagu uuccucuuuu cgcugaccca gguuacacug auaaagagua uuacaaggaa      360

uauccgacca ucuaccaucu gcggcaagag uugauuaaaa accccgaaag ccacgacauc      420

cgccucgugu accuggccug ucaccauaua cugaagaauc gagggcacuu ucucaucaac      480

ggauccuucu cugauuuaaa gaaucugucg uuuguaugca gagagaugcu ugacgcauuc      540

aacgaguugu caaaguauca gcugaugauu agcgauaucg acgcccuaga aaagauucuc      600

agugauagga aacagcagaa guccgcuaaa gugaaggagc ugguccaaau cuuugaaugu      660

gugccucggg aggacaaauc ugacgcaaag auaugcaaag aaauucuuag caaucugugu      720

aaguucuugg uuggcgccaa gggcaacauc acacgucucu uugauaccga cuucgaugag      780

auggagacuu ccucauucag uuuuggggac agcaaauacg aagauucucu guuaccaucc      840

cuggaggaaa auuaucccga cuacgcuaac gugauucaga agaucaaagc cauguaugau      900

uggucagucc ucguggagau cauggcggga gaaaauuacc uuagcuucgc aaagguaaac      960

gccuauaaaa agcauaagga gaaccugagu auuuugaaau cucuaaugaa gaaauacugc     1020

acaccugacg uuuaccagcg cuuuuucuau gagaccgaug aaaaguccag cuacgguaau     1080

uauauaggcg cuauccacaa caaugggcac ucgcauacga ugaaaaagug ugacgaggaa     1140

acuuuuuaca agucucuguc caaaauucuc gauaagcugg ugguccagaa aaccgaccaa     1200

gacacacuug agauccugaa gaaagaagcc aagaaccaga cuuugcuccc acuggcaaga     1260

accaaggcua auggagugau acccaaccag auucacaucc aagaguuaag ggccauccug     1320

gauaaugcga aaacauaucu aucauuccuc aacgaggccg acgaauacgg caccauugca     1380

gagaagauca uucggauagc uaaauuuaag aucccuuauu acgccggucc acugaguacg     1440

cgacauaaag aacagggggc uaauacuugg auugugcgca agcaggaggg aagaaucuac     1500

ccguggaacu ucgaggaaau ggucgaucuu gagaggagca auaacgaauu uauccggcgc     1560

augacaaaua aaugcaccua uuugauuggc gaggacguuc ugcccaagaa cucccuccug     1620

uacucuaagu ucauggugcu uaaugaacug aacaacuuga aaauaagagg ccacaagauc     1680

agcguagagc ucaaacagga gcuguauaag gaauuauuuu guaaaaaggc caaggugacu     1740

gggaaagcac ugcuagucua ccucaagaaa aaggaugccg agcugacacu ugaagacauu     1800

ucaggauucg augcugacuu uaaaaguucc uugagcaucu auauugauuu caagaaucac     1860

auccugaggg agcaaauaga cgaugguaag uaccagucua ucguggaaaa cauuaucaaa     1920

uggaagacca uuuucgacgu ugauucaaaa augaucgaga agcucauagc gcgugaguau     1980

ccuaauaugu uuaguagcga acagauuaaa aagaucugcc ggcugaagug ucgaggcugg     2040

gggaacuucu ccucuagauu uuuaaaugga auacagggcg agaacacgga aaccaaagag     2100

ugcuucacaa ucauagaagc acuguggaag acuaauuaca accuuaugca auugcucucg     2160

aaacaguuua ccuucaagga cgccaucgac ugcauuaaug cugauaaagu ggguaagauc     2220

gacaaaguca cauacgagaa cacugugaag gaucugguag uuagcccagc caauaagagg     2280

gcaauuuggc agaccgugca gaucacagag gaaauuaaaa aggucaugaa augugagccc     2340

gaaaagauuu uuguggagau ggcccgcggg ggcgagaaag aaaagaagcg gaccaucucc     2400

agaaaagaga ggcuacugaa gcucuauaug gacuacaaag augaaaaccg cgacuggauc     2460

aaggagauug aaucacgaga ugagcaagac uucacuaauc ggaaacugua ucuuuacuau     2520

acgcagaucg gacguugcau guacaguggc gaggaaauuu cuuuggagga acugaugcag     2580

aagaacagca agugggauaa agaccauaua uauccucagu ccaagaucaa agaugacuca     2640

uuagauaauc ucguccuggc uagaaaggac auuaacaaca auaaaagcaa caaacuucug     2700

ccaacaggga uccaagacaa gaugaaaaag uacuggaaaa uguugcucaa guacggauuu     2760

auuucuaaaa agaaguauga uaggcugaug cgcaccgagg acuucuccga ugaggagcua     2820

agugguuuua ucgcccggca gauaguggag acuagacaga gcucuaaagc aguugcggaa     2880

cugcucgagc accugaauga acauaccgag aucguguacg uaaaggcuga acuugucucc     2940

gacuucaggc acgauaacaa uauguugaaa ucacgacgca uuaacgacua ucaucacgcc     3000

aaggaugcau uucugaauau cgugguuggc aacguguaca augccaaauu cacagcuaac     3060

ccccggaauu ggauuaagaa aaacagaaau accaacuaua guaucaggcg ugucuucgac     3120

uacacuguga agcgcgggag cacaauagua uggcaaggag ugaaggaugg cggucacucg     3180

auugacggga ucaaaaagac gauggcccgg aaugauaucu uauauaccga gagaacuuac     3240

ugugagcagg gcgaacucuu uaacgcaaca cugauuaaaa agggaaaccc gaaugcuaua     3300

ggccuuaaaa aggggcugga cccuaugaag uacggagguu auuccucucc caaaaccagc     3360

gccuucgcgu uuaucgaguu cgacggcaag aaagggaggc agaaccaucu aguugaaauu     3420

ccagucuaca ucgccaauau ugcugcacga cagccugagu auaucaagaa ccaccucgag     3480

gagaaaaagg gauaccaaaa ugugauagug aagaaauauc ccauccagaa gaacgcccug     3540

auuaaauugg auggcuaccc agcucggcug cgcggugcca augaaaaguc aaucacacuc     3600

aaaaacgcag uccaguuagu ucugaagccc gaauaucaug agacuauuag aaauguggaa     3660

aaguaccuug aguacaucga caaagaugag agugugucca aggcggccag guuuucucac     3720

accgaacugg acaaauugua ugaugagcuc ugcuucaagg cugaaaacag cauauacaaa     3780

aagcggccug ccaaucaguc cgcaacgcug aaaaacgggc aaaagaccuu ucuagcuauu     3840

acaaaucuga aggagaaagc ccagguaauc aacaauauca uuacuauguu ccgaugugac     3900

gcaaccacaa cuaccaaccu uaagacgauc ggcggaucag ccaaugcugg cgggaugcag     3960

gucaacaaaa acacacucac cagccagaag gugguucugg ugcaccaaag uauaacugga     4020

uuguuuggca cagaagagga gcuguuauga                                      4050


<210>  3023
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3023
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

guuacagaug acuauaaggu gccugcuaaa aagaugacug uacucgguaa uacggauaaa      120

ucucacauaa agaagaaccu ucugggcgca uuguuauucg acucagggaa uaccgccgcg      180

gauagaaggc ugaaacggac agcucgccga cguuacacua gaaggcggaa ccgcauccua      240

uaucuccagg agauuuuugc cgaagagaug aguaaggugg acgauagcuu cuuucauaga      300

cuggaagacu ccuuccuugu caccgaggau aaaaggggag aacggcaccc aaucuuuggc      360

aaucuggagg aggaagugaa guaccaugag aacuucccga caauuuauca cuugcgccaa      420

uaccucgcag acaaucccga aaaaguugau cugcgacugg uguaucucgc cuuagcucau      480

aucauaaagu uuagagguca cuuccugauc gaggggaagu uugacaccag gaacaaugau      540

guccagcggc uuuuccagga guuuuuggca guguacgaca acacuuucga aaauucuucg      600

cugcaggagc aaaacguaca gguugaagag auucuaacag acaaaaucuc aaagagcgcc      660

aaaaaggauc gugugcucaa acuguuuccu aaugaaaaga guaacggacg cuucgcugag      720

uuccuuaaac ugauugucgg caaucaggcc gacuuuaaga agcacuucga guuggaagag      780

aaaaccccac uccaguuuuc caaggauacg uacgaagagg aacuggaggu guuacuggcg      840

caaaucggcg acaacuaugc agaacucuuc cuuucugcca aaaagcugua cgauagcaua      900

uugcuauccg ggauucugac ugugaccgac gucucaacaa aagcuccccu cagugccagc      960

augauccaga gauauaacga acaucagaug gaucuggcac agcuuaagca auuuauuagg     1020

cagaagcugu cugacaaaua caaugagguu uucuccgaug ugucaaagga cggauaugcu     1080

gguuacaucg auggcaaaac uaaccaggaa gccuuuuaua aguacuugaa agggcuccug     1140

aauaagaucg agggaagcgg cuacuucuua gacaagauug aacgggagga cuuucugcga     1200

aaacaacgca ccuucgauaa cgguaguaua ccucaccaga uccaucuaca ggagaugaga     1260

gcgauuauca ggcggcaggc cgaauuuuau ccauuccucg cagacaauca agaucgcaua     1320

gagaagauuc ugacauuuag aauccccuac uauguagggc cucuugcuag gggaaaaucu     1380

gacuucgccu gguugucccg uaagagcgcu gauaaaauca ccccauggaa cuucgacgaa     1440

auuguggaua aggagucguc ugccgaggca uuuaucaauc ggaugacgaa cuacgaccug     1500

uaucucccga aucagaaggu ccugcccaaa cacucccuuc uguacgaaaa guucacugug     1560

uauaacgagu ugacaaaagu uaaguacaaa accgaacagg gcaagacugc cuuuuucgau     1620

gcuaauauga aacaggagau uuuugacggc guguucaagg ucuaccgaaa ggugacaaaa     1680

gauaagcuca uggacuuucu ggaaaaagag uucgaugagu uuagaauagu agacuuaacc     1740

gggcuggaca aggaaaacaa agcguucaau gcaucauaug gaacguacca cgaucuaagg     1800

aagauccucg acaaggauuu ucuggacaac aguaaaaaug agaagauucu ugaagauauc     1860

guuuugaccc ugacacucuu cgaggaccgc gaaaugaucc ggaaaagacu ggagaacuau     1920

agcgauuuac ugacuaagga gcaagugaaa aaguuggaaa ggcgccauua caccgguugg     1980

ggccgacuuu ccgccgagcu cauucacggg auacggaaca aggaaucucg uaaaacaauc     2040

cuggacuauc uaauugauga cggaaauagc aacagaaauu uuaugcagcu gaucaacgau     2100

gacgcucucu cauucaagga ggaaauugcc aaagcacagg ucaucggcga gacugauaau     2160

cugaaccagg uggucaguga cauagccggu uccccugcua ucaagaaagg gauucuucaa     2220

ucauugaaga ucguggacga gcugguuaag auuaugggcc aucagccaga aaauaucgug     2280

guagagaugg ccagggaaaa ccaguucacc aaucagggac gccggaacuc ucaacagaga     2340

uuaaaaggcc ucacagauuc aauaaaggag uuugggaguc agauucugaa agagcacccc     2400

gucgaaaaua gccaacuuca gaacgacagg cuguucuugu acuaucucca gaauggacga     2460

gauauguaca ccggugagga acuggacauc gauuaccuau cccaguauga cauugaucau     2520

aucauaccuc aagcauuuau caaggacaac ucuauugaua aucgcgugcu gacuucgagc     2580

aaagagaacc ggggcaaguc cgacgauguu ccaucagaag acguggucaa cagaaugagg     2640

cccuucugga auaaacuccu gaguucuggg cuuaucagcc agcguaagua caacaauuug     2700

acgaagaaag agcugacacc ggacgauaag gcgggauuua uuaaacgcca guuaguggag     2760

acccggcaga ucacuaagca cguagcuaga augcucgacg aaagguucaa caaagaguuu     2820

gaugacaaua acaagcgaau acggcgcgug aagauuguua cccugaaauc caaucuuguc     2880

agcagcuuca gaaaggaauu ugagcuguau aaagugaggg aaaucaacga uuaccaccau     2940

gcccacgacg cauaucuaaa ugccgugguc guuaaggcuc uccuggugaa auacccuaag     3000

uuggagcccg aguucgugua uggcgaauac ccaaaguaca acucuuaucg ggagcgaaaa     3060

acagccaccc aaaagauguu uuucuacucc aauaucauga acauguucaa aaguaaggua     3120

aaacuggcag augaccagau ugucgaaaga ccuaugaucg aggugaauga ugaaacuggu     3180

gagauugcuu gggacaagac aaagcauaua acgaccguua aaaagguguu aagcuauccc     3240

caggucaaca ucgugaaaaa gguugaggaa cagacuauug ggcaaaaugg cggacucuuu     3300

gaugacaacc caaaaucucc ccuggaggug aucccuucca aacuuguacc acugaagaaa     3360

gccuugaacc cggaaaagua cggcggguau cagaaaccca caaccgcgua cccuauucuc     3420

cugaucgucg auacaaagca gcuaauacca aucucaguga uggacaaaaa gcgcuucgag     3480

caaaaucccg ugaaauuucu gaaggacaag ggauaucagc agauugagaa aaacaauuuc     3540

gucaaacuuc cuaaguacac ucucguugau aucgguaacg gcauuaagag gcugugggcc     3600

aguagcaaag aagugcacaa ggggaaucag uugguagugu cgaagaaauc ccaagaccug     3660

uuauaccacg cucaucaccu cgauaacgac uauucuaaug aguacgucaa gaaccauuau     3720

cagcaguuug auauccuguu caaugaaaua accagcuuuu caaagaagug caaacuugga     3780

aaggagcaca uucagaagau cgaagaggca uacaguaaag agcgugacuc cgccucuauc     3840

gaggagcugg cugauggcuu cauuaaguug cucgguuuua cgcaacuggg ggccaccagc     3900

ccauucuccu uucuaggcau aaaacugaac cagaagcagu auacagggaa aaaggacuac     3960

cuucuccccu guauggaagc aacucugauc caucagucaa uuaccggcuu guaugagaca     4020

cggaucgauu uaagcaagcu gggaggagac uga                                  4053


<210>  3024
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3024
augggcaaga acuacuaucu ggggcucgac augggaacca gcuccguggg uugggccguc       60

acagaugaga aauacagaau ccuuagggcu aagggcaaag accugugggg aguucgguug      120

uucucugaag caaagacugc cgcggagcgc cgaacgcacc guaccucaag aaggcgguua      180

gaucgcgaaa aagcuagaau ugccugccug aaggagaugu uugcagaaga gauaaauaag      240

aucgaccccg gguucuauca gaggcuaaac gauaguaaau acuuucucga ggacaagaaa      300

gaacaucaac cuuaugccau uuucgcugau acaggcuaca cugacaagga guauuacgau      360

gaauacccaa ccaucuuuca ucugcggaaa gcacuucuga auauugagaa cgacaagaag      420

aaauaugaug ugcgcuuggu auaccucgcc auccugaaua uguucaagca ccgaggucac      480

uuucugaacg cuaaucucga cgauaaaagc gggggaaacu uaaaggagua uauagaaaug      540

cugcaucagu ccauggugaa uauccuuaac auuaauuuga aaaacgucga gaucgaaaag      600

auugagaaaa uccugaaguc uacacaguac agcaauucag aaagacuaga gaaccuccug      660

gagcuuuaug aacugagcaa gaauaaaaac aagauagaga ccgaaauuuu caaauuuauc      720

uguggcuuga agggcacuau uaguaaaauc uacguggagg acaaguucga agacgaguuu      780

gccaagauga cacucuccuu cagggauucu aacuuugacg agaaaaucau ugaaauagag      840

gauaaucugg acgaugacaa guaugauaug uucuuacuga ucaaacagau ucacgacugg      900

agcguucucg cgaacaucau gaauggggaa gaguaccuuu ccguggcacg ggucaagcug      960

uacgauaaac acaagaagga cuuggaagug uugaaaaagu auuacaaaca aaacucaaug     1020

gaggaguaua auaagauguu ccgucagaug aacgauggaa auuacagugc cuauguaggu     1080

agcguuauau acaaagacaa cucugugcgc agaggcugca aguccaagaa agaagacuuu     1140

uauaagaaua uucugaacac caucaaauca ugggaggauu gugaagcuaa ggaguacauc     1200

acgagcgaga uugacaaagg gaauuuccuc ccgaagcaga ucacugccag uaacggaguc     1260

auucccaauc aggugcauaa aaaggaacug aagaaaauac uuaagaacgc aucugaguac     1320

cugaauuuuc uaaacgaaaa agaugagucc ggcuauacca ucagcgaaaa gauugugaaa     1380

cucuucgagu uucaaauccc uuacuauguc gguccaaucg cuuacaacau ugggaaugac     1440

gagucgaagc acaggcauaa cauguggucu guucggaagg aaaaaggacc cauauauccu     1500

uggaauuucg agcagaagau cgauauuaaa aaguccucag aaaaauuuau ccgaaaccug     1560

auuaaucacu gcacauacuu aaacgacgag gaagugcugc caaagaauag ucuacucuau     1620

gagaaguuca ugguacugaa cgagcuuaau aaauugaaga ucaacggcga gaaaauaagc     1680

guggaacuga agcagaauau cuuuaacgau cucuucaaaa agggcaagaa agucacuaag     1740

aaagggcuga uuaaguaccu uaaagaacag ggagagaucg acaauuguga agagguggag     1800

auuuccggua ucgauggcga cuuuaccaac aagcugucua auuacaaaaa guucgccgau     1860

auauuugggg uucaaucguu gacauaugaa cagaccgaca uugcggagaa caucauucgc     1920

uacucaacga ucuauggaga uaguagaaag uuccucgaag agaggauacg ggaggaauac     1980

uccaacgugc uggacgagaa acagaucaag cgcauuuuag gcaugaaauu caaggacugg     2040

gguagacuga gcaaagaacu acucgagcug ucuggggucg auaaggaaac uggcgagauc     2100

gccucaguga uuaguaggau guggaaugac aacuauaauc uuauggaguu gaucgcaaca     2160

gaacguuuua gcuacgcuua ugagauagaa caacggucca agaaacugga gaagaccauu     2220

ucuacuaucg aauacgagga ucucgacgag cuguacaugu cgacacccgu aaaacgaaug     2280

guuuggcaga ccaucuuagu gcugaaggaa cuugaggaag ucuugggaug cccuccaaaa     2340

agaauuuucg uggagauggc cagggauguc aacgcuccga aggaccgcaa ggauagccgg     2400

aaaaagaaac uccuggaacu auauaagaau uguaaaaagg aggagggcga cugguccaag     2460

gaacugaacg ccagagagga aucagaguuu aaaaguaaga aacucuaccu guauuacaug     2520

cagaagggga ggugcaugua uucuggaaaa cccaucgauc uuaagaauuu gcugaacgac     2580

aauuuauacg auauugacca cauauauccu cgccauuucg ugaaggauga cagcaucgag     2640

aacaaucucg uucuggugga gaaaaacuuu aaugcacaca aguccgacaa cuacccaauu     2700

gaaaaugaaa uccgacagaa aaugcauuca auguggaaga ugcuucggga ugguaacuuc     2760

auuacggagg aaaaauacag ccgucugauc agaaaggagg aguuuaccga cgaugaaaaa     2820

gccaaguuca uaucuaggca aaucguagag acacgccagg gcacuaaggu cauuaccgac     2880

uuguuugaac agacauuccc cgaguccgau aucguguaug uuaaagcugg gaacgugagu     2940

gaauuucggc acaaguucaa acucauuaag uguagaaaug ucaacgacuu ucaucacgcg     3000

aaugaugcau accugaacau cgugguagga aauguguauu acacuaaauu caccaagagc     3060

gcccuaaacu ucauaaagga cuauaaaaag aauccugaga aaaacaagua ccacauggau     3120

cauauguuuc aguauccagu uucuaggggc ggugucgacg cuuggauuac aaaaggggcc     3180

gaguccaucu caauggugcg aaagaugaug gcaaagaaua cccccauggu gacucgcaug     3240

aacuacgaaa aacacagugg ccaaaaggga ggcaucagcg aucagaauau uugcucggcc     3300

aaaauagcua agaaaaacgu cuaccugccg aucaagaugg gggacgagaa gcucaaaaau     3360

guugaaaagu auggagguua cggcucccug acggccaaau auuucuuucu uguggagcau     3420

acaauuaaga aaaagcggau cagaaccauu gaagcauacc cuauguauuu ggacgcgcug     3480

aacaaagaga auaaguuacu caacuacugu gugcagguac uggaguauga ugaacccaag     3540

aucauaaugg agaaaaucaa ggucaaaucu cuuuucaaga uuaaugggua cuacuauuac     3600

cugaacgcua aaagcgugaa caggaucauu uggggaaaug ccacucagcu auuucucgaa     3660

aacgacauca augaguaugu ucguaagaua ucaaagaaca uugagugcau cgauaucaau     3720

agugaaaaca auauuaaagu guacgacauc uucauagaga aguauuccaa caaacuguuu     3780

ucuaaucgcu ugggcaaccu gaaggaagca auuauccaaa aucggaacaa auucauugag     3840

uuaaagauca augaacagau aaacgucauu cucgagcugg ugagauacag ccaggauggu     3900

guuuauucca ucaaucuuuc acagcugggg uugagcagua ccuacgccau caacaggaca     3960

ucuaaggaga uuuccaaaau gaaggaaugc aaacucauca accaaagcgu gaccggccug     4020

uacgagaaug aaauugaccu acugacugua uga                                  4053


<210>  3025
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3025
augcagcaau acuaucuggg cguggacaug gggagcgccu ccgucggaug ggcuguuacc       60

gaugagaagu accagcucgu gagaaagaag gguaaagacc uuuggggcgu aaggacauuc      120

gauaucgcac agacugccga agugcggcgc gucucucgaa cgaaccguag aaggcagaau      180

cggcgcaagc aaaggauuca gauacugcag gaguuguuag gggaagaggu gcugaaaauc      240

gacgcgggau uuuuccacag aaugaaggaa ucacgguaug uugcugagga uaagcgcacc      300

cuagacggca aacaggugga gcuccccuac gcccuguuug ucgaucaagg uuucacagac      360

aaggauuuuu auaaacaguu cccuacuauu aaccaucuua ucguguaccu gaugaccaca      420

agugacaccc cagauauucg auugguauau cucgcacugc acuacuacau gaagaauaga      480

gggaacuuuc ugcauagcgg agacaucaau gauguuaaag acauacaguc cauccucgaa      540

caguuagaga acgugcugaa ggaauauguc gacgauuggg agcuuucuuu gaaggacaaa      600

guggaugcca uuaaggagau cuacaauaaa gaccugggca ggggcgaacg gaagaaggcu      660

uucauuaaca cucuaggggu gaagacaaaa ucggcaaagg ccuuuugcuc acucaucagc      720

ggagguagua ccaaucuggc ugagcuuuuc gaugacuccg gccugaagga aucugaguau      780

gccaaaauag aauuugcgaa cgcaaauuuc gaggauagcg ucgaggggau ucaagccuug      840

cucgaagacc guuuugcugu uaucgaggcc gcaaagcgcc uguacgauug gaaaauuuua      900

acggacaucc ugggagauaa cgcuucccuc gccgaagcga gagugaaguc auaugagacu      960

caccaugaac agcuuguaga gcugaaaagu uucaucaaga aguacuugga caggaaaauu     1020

uaucaggaca uauucaucaa uccgaacauu gccaauaacu accccgcaua cgugggccac     1080

accaagauca acgguaaaaa gcaagagcua gaagucaaac gggcuaagcg aaaugauuuu     1140

uaugccuaca uaaagaaaca ggugauugac ccuaucaaga aaaagguuag cgauaaagcu     1200

gugcuggccc gccucgcaga gaucgaaucu cugauugagg ucaacaagua ucuuccacug     1260

cagguaaauu ccgacaacgg ggugaucccc uaccagauua aguugaauga acucagaagg     1320

auauucaaca aucuggagaa ccgguuaccu guucugaaag agaaucgcga uaagaucauu     1380

aaaacauuuu cauauagaau cccauacuau gugggacccc uaaacggcgu caauaggaac     1440

ggcaagagca cuaauuggau ggugcguaaa gaaggggagg aaggaaagau cuacccuugg     1500

aacuucgagg agaaagucga ccucgaagcc agugcugaga aguuuauucg gcgaaugacc     1560

aauaagugua cauaccuggu gaacgaagau guucuuccaa aauauucuuu gcuguacucc     1620

aaguaucucg ugcugagcga gcuuaacaau cugagauugg acgguaggcc gcucgaagua     1680

ucggucaaac aagagauaua cgagaacgug uucaagcgca aucggaaagu uacccugaag     1740

aagaucaaaa acuauuuacu gaaggaaggc gugauuucug agaaagauga acuauccggg     1800

cucgcggacg augucaaguc aagucugacg gcauaccacg acuuuaaaga gaaacuugga     1860

cauuugacuc ugacagaaga ucagauggag aagaucauuc ucaaugugac ccuguucggc     1920

gacgacaaaa aguuacugaa aaagagacuu gccgcuuugu aucccaacau cgaugagaaa     1980

agccucucca ggauggccac uuuuaauuac cgcgacuggg gucgacuguc uaagaaguuc     2040

cuaagcgaaa uaacaucagu agaucaggag accggggaac ugcggacgau cauucagugc     2100

auguacgaga cccaaaacaa ucucaugcag cugcuuagug aaccuuauca cuuuguggag     2160

gcaaucgaga aagaaaaccc aaagguugac uuggagucca uuagcuaccg uaucgucaau     2220

gaucuguaug ugucucccgc cgugaaaaga cagauauggc agacauuacu cgucauuaag     2280

gacaucaaac aaguuaugaa gcaugauccu aaaaggauuu ucaucgaaau ggcucgcgag     2340

aagcaggagu caaagacuac caaaagucgg aaacaggugc ugagcgaagu guacaaaaac     2400

gccgagaagu auaaaaaucu uuuugaaaag cugaacuccu ugacagagga acagcucaga     2460

ucuaagaaag uauaccugua uuucacucaa cuaggcaagu guauguacac caaugacgca     2520

auagauuucg agaaccuggu cucggcgaac agcaauuacg acaucgauca cauuuaucca     2580

caguccaaaa caaucgacga uucauuuaac aaucucgugc ugguuaagaa aggaauuaac     2640

aaugacaaga gugacaggua ccccaucgau aagaacauac gagacgauga gaaagugaag     2700

acccuuugga auacuuugcu gucuaaaggc uuaauuacga aggaaaaauu cgagcgccuc     2760

auccggagca caccguuuuc cgacgaggaa cuggcugggu ucaucgccag acagcuuguc     2820

gaaaccaggc aaucaacuaa ggcaguggcc gagauucuga gcaacugguu uccugagucu     2880

gaaaucguuu auuccaaggc uaaacacauu accaauuucc gucaggauuu ugagauacua     2940

aaggugcgcg aacucaacga cugccaucac gcccaugaug cauaccugaa uaucguaguc     3000

ggaaacgcuu aucacacaaa auucaccaau agucccuacc gguuuauuca gaacaaggcc     3060

aaucaggagu auaacuugag aaaacuguua caaaaggcga agaaaaucga aagcaacggu     3120

gugauugccu ggaucggcca gucugagaau aacccaggga cuauagcuac agugaagaaa     3180

gucaucucca ggaauacggu ucucauuuca cgaaugguga aggagguaga cggacagcug     3240

uucgaucagc aacuuaugaa aaagggcaaa ggucaggugc cuaucaagag uagcgacgau     3300

cggcugauug acaucucgaa guacgggggc uacaacaaag caaagggagc cuauuuuguc     3360

uucauaaaau ccgugcgcag aggcaagacc auuaaaucuu uugaauacau ccccguucau     3420

uuggcuaaga aguucgacug uaaucucgag cugcuaaaag aauaucugga gagcgagaag     3480

gaucuuaaca auguggaaau ccucaugcca aaagucauga uuaacucacu guucaauuac     3540

aacgggaguu ugauaaggau ccccggacgg uaugacaaga aaucccuguu aauuaaugug     3600

gauguuccuc uccugcuuga gucucagcac aucaagcagc ugaaggugau ugaaaaauac     3660

auguauaaga aacgagucag caagaacucc aauaucuugc ucacuaaauu ugccucagac     3720

caacugaagg aucuagacgc acuguucgau guacuuagcu acaagcucaa cgagaauaua     3780

uacaacguga ucaaugacaa auaugauaag cugguuauuu gcagagacaa auuuaucagu     3840

uuggauacag aagugaagug ugagaugauu uucgaguuac ugcaccucuu ucagugcaac     3900

ucucagcugg cgaacaucac caaaauaggu gccacaucca aguucggcag cauuucgaug     3960

ucuaaaaauc uuaaggaaaa cgacaagaug ucaaucaucc aucaaagucc auccgggauu     4020

uuugagcacg aaaucgagcu gacugcuuug uga                                  4053


<210>  3026
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3026
augaagagaa uccugggccu cgaccuuggg accgccagca uuggaugggc ucuggugaac       60

gaguccgaaa augagaaaga aaagucugag auaaucaaau ugggugucag gguuaacccc      120

uuaacaacug augaacagac ggacuucgag aagggccggc cucugucagu gaaugcagau      180

cgcacccuaa aacgaggggc ccguagaaac cuccaaaggu uuaagcagcg gcgcaagaau      240

cugauugaga uccuuauuaa aaagggauuc aucacaaaca uaagucuguu gacugaaauc      300

ggcagcaaua ccacauacca gacccucgag cugagagcga aauccguaaa ggaaaagauu      360

gagaaggagg aauuugcuag ggugcugcuc acuaucaaca agaaacgggg uuauaagucu      420

ucgcgcaaag ccaagaauga cgaggaaggg ucauuaauug auggaaugag cgucgcaaaa      480

gagcuguacg acaacaaucu uacagugggc caguauguuu accaauugcu ggaaaagggc      540

gggaaauaua ucccagauuu cuaccgaagu gaccuacaga acgaguuuga ugugauaugg      600

aauuuccaga aggaguuuua uccgaacauu cucaccggag aacuguacaa gaaacuucag      660

ggucaaggcc agcaaaauuc cagaaagcug uucuuggcca uccaccagau uuacacggcu      720

gagaacaaag ggaagaggga cacugucaaa aagcagcauu auaaguggcg gaacgaugca      780

aucaauaccc agcuccugau cgaagaggug gccuacguau uaguugaaau uaacaauaac      840

cugaauaaau cuagcggaua ucucggcgcu auauccgacc guucaaagga gcuuuacuuu      900

aacaaagaga caguggguga aaaucuguau aagcagaucc aagagaaccc ccagacuagc      960

uugaaaaauc aggucuucua ccgccaggau uaucuagacg aauuugagca aauuugggaa     1020

acccaggcca aguucuacag cgagcugaca aaggagcuca aagaagaggu gagagacgug     1080

gucaucuuuu accagaggaa gcugaaaucu cagaagcacc uuauauccga augcgaguuc     1140

gagaaacauc acaaggcgau uccuaaguca agcccacugu uccaagaauu uaaaaucugg     1200

cagaucuuga acaauauuga guucaagcau aaaaagacca aagaacucaa gauccugagu     1260

gaggaauuaa aagagacgcu guuugaggaa cuaaacauuc gggggaauuu cacuucugau     1320

gcaguucuca aguuugugaa gcuggacaaa aaggaguggg aacuuaacua uaaaaaggga     1380

auagagggca aucgaacaaa cgaaguauug uacaacgugu aucagaaaau cgccgaguac     1440

gagggcuaug gguucgauug guccaaaaag agcgcugucg aaauuaaaga ggaacugaau     1500

accaucuuuu cggagaucgg aauuaacucu aagauacucg acuucaaucc cgaaaucucc     1560

gguaacgauu uugacaaaca ggagucauac caacuguggc accuucugua uagugccgag     1620

gaagauggcu ucuugaagca gaaacuccag gacaaguacg gguuuaagag cgaguacgca     1680

aauaugcugg cuaacguguc cuuccagucu gauuauggaa guuuaucagc ccgcgcgauu     1740

aaaaagaucc ugccuaaucu aauugccggc cacgaauuua gugaggcaug uucccucagc     1800

gguuacaacc auucuaauuc acugacuaaa gaggaacuug cuaacagacc auugaagggg     1860

aaacuggagc uccugaagaa gaauaguuua aggaaccccg uuguggaaaa gauccugaau     1920

caaaugguca accuugugaa ucagguaaua gagagcuaug gcgaaaucga cgagguucgg     1980

auugaguugg cccgcgaacu caagaaaucc gcucaggaga gagcccugau gacaaccaac     2040

aucaacgcau cuaagacgaa auacgaaucg uucaggaagc uacugcaaaa agauuucgga     2100

auuaagaauc cuacccguaa cgacaucaua aaguaucggc ucuacgagga acugaaagcc     2160

aauaaguauc acacaauuua cacuaacaaa uacaucccac gagacaaacu uuuuagcaaa     2220

gagauugaua ucgagcauau aaucccgaag gcuaaguugu ucgacgauuc cuuuucaaau     2280

aaaacccugg cguucagaaa gcccaaccag gacaaaggca auaugacagc aauugauuau     2340

aucuuaaguu accagucuaa ggacauugau aacuauaaaa gcaggaucga aaaucucuuu     2400

aagaacggga agggagacau auccaaagcc aagcgcaaua aacugcuuau ggaggaaaag     2460

gauauuccug acgguuucau cgaucgggac cugagaaacu cacaguacau cgcuaaaaag     2520

gccaagcaau ugcucgagga agugauuagg acugucaaua ccacaaccgg cagcgugacu     2580

aacaaacugc gcgaugacug ggggcuaauc aaugucaugc aggagcugaa caugccaaag     2640

uuuaaagcaa uugacgaguc ucucauagaa cagaucgaga agaaagaugg aacguucaag     2700

acacgaauua aggacugguc caaacggaac gaucaccguc accaugccau ggacgcucug     2760

accguggccu uuacuagaua uaaucacauc caguaccuua acaauuugaa cgcaaagagu     2820

gauaaaaagg gcgcgauuua ugguauccaa gaacuggaga ccuacguuaa uagcaaaggg     2880

aagaggaaau ucauaaagcc caugcauaac uuucgcgagg aagcuaagaa acacaucgag     2940

gacauuuuaa ucucuuucaa ggccaaaaau aaggugguaa caaagaacau uaauaagauc     3000

aaguccaaag auggcuuuau aaagaaaguc cagcucaccc cucggggaca gcugcauaag     3060

gaaacugugu acggcaaaau uaacgagguu guggucaagg aagagaagau caaugcaaaa     3120

uucgaccuug agaagaucaa caaagugaca aagccaauuu auagaaaugc ccugcuaaaa     3180

aggcuccagg aguucaacgg ggaacccaag aaggcuuuua cggguaaaaa uucacugagu     3240

aagaacccga uauacuugga uguagcccaa accaaaacuc ugccugaaag cgugaagguu     3300

caguucuuug aggaaaauua uacaauccga aaagagauuu cgcccgacuu aaagaucgau     3360

aaaauuaucg acaagggaau acagcgcauc cuccugaagc ggcuugauga guucaacggc     3420

aaaccaaagg aagcauuuuc caaccuggag aaaaauccua uuugguugaa caaggaaaaa     3480

gggaucgcua uuaagagagu caagaucaaa ggagugaaga auguggaguc ucuccacaac     3540

aaaagggacc accugggcaa ugaaauacua caaaagggua aaagcgcccc caacgacuac     3600

gucucaaccg ggaauaacca ucacguugcg auuuaucagg auaagaaugg caagcugcag     3660

gagaaagugg ugaguuucua cgaggccgua gcucguguca acaauaaacu uccaaucauc     3720

gaccaggauu uuaacaaaga ccucggaugg aaguuccugu uuacaaugaa acaaaaugaa     3780

auguucgugu uucccuccga ggauuucaag ccuuuggaaa cugaccugau ggauaagaaa     3840

aacaauucua agauuagcaa aaacuuauuc cgcguucaga agcucggcaa caaagacuau     3900

accuuucggc aucaccugga gacgaagauc gaggauauaa agaaacuuag agacuucacc     3960

uacauuagga agcgaaaucc agaauccauc aaaaacauua ucaaggugcg gauaaaucac     4020

cuggggaaca uuguccaugu gggagaguac uga                                  4053


<210>  3027
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3027
augagcaaga aauacuaucu gggccucgac aucgggacca acuccguggg augggccguc       60

acagaugaga auuacaaccu uugcaaguau gcugguaaaa gaaugugggg cauuaggcug      120

uucgaagggg gagagacugc agaacagcgg cgcauaucuc gaucaaaucg uagaagguug      180

agucggaaga aacaacgcau cgacuuacug caggagcuau uugccgaaga gauugcgaag      240

auggauccca cguucuuuau cagacucaac gagagcaggc ugcaccuuga agacaagucc      300

accggcgaga aauucccucu guuucgggau aaggacuaca cagauauuca guauuacaaa      360

gaauauccaa cuauguacca uuugcgcaag gagcucaucg aaaauucuaa aguucacgac      420

auacgacugg uguaccuggc uugucaucac auccucaaga acagagguca uuucuuaauu      480

aaugggucgu uuucagaugu aaagaaacug agcagugugu gcgaauccau gcuuaacucu      540

uucaauggaa ucagcaagua ucagauugag aucaacgaca uagaaagguu ggaggaaauu      600

cuguccgauc ggaaaaagca aaaaucaguc aaggccaaag agcuaaucca gauuuuugag      660

uguggcccgu gcgaagacaa gaccgaugag aagacaugua aagcaaucgu gaguaaucuc      720

ugcaaguggc ugaccggccu uaaagggaac aucaauagca uuuucgacau gcaguuugaa      780

gagauagaca cuucuuccuu cucauuuaca gaacccaagu acgaggauac caucagcgcc      840

gcucuggagg aaaaauaucc ugacuacgca gauguuauuc agaagaucaa ggccguguau      900

gacuggagug ucuuggugga gauaauggcu ggagaagauu accucucuuu cgccaaagua      960

aacgcguaua auaagcacaa agagaaccug uguauuuuaa aggaaguuau ccguaaauac     1020

ugcgacuccg agaucuacca caaguucuuu aacgguuucg augagaagaa uggcuauggg     1080

aacuacauug gaagcaucua uaaaaauggc cauucguacg caaugaagaa auguucuacg     1140

gaagacuuuu auaagacucu ggguaaaauu cucgauaaga uacaaccaga gaaggaagac     1200

gagaacgugc uugaggaacu gaaagucgag gugaagaauc agaccuugcu accccuggcc     1260

cgcacaacug cuaacgggac caucccuaau caggugcaca uucaggaacu cagagccauc     1320

cuggauaacg caaagaagua ccuuccauuc cugaaugagg aagacgagua uggaacaguc     1380

gcugagaaaa ucauuuccau agccaaguuu aggauccccu acuacguugg cccuuuguca     1440

acccggcaua aagaacaagg cgcgaacagu uggauugugc gaaagcagga ggggcgcauc     1500

uauccaugga auuucgaaga gaugguagac cucgaacaga gcaacaagga guuuauucaa     1560

agaaugacga auaaaugcac uuaccugauc ggagaggaug uguuaccgaa gaacucccug     1620

cuauauucua gguacauggu ccucaaugaa cugaacaacc uucggauacg cgguaauaaa     1680

aucagcguga aguugaaaca ggagcuguau aaggaacucu ucuguacaaa ggccaaaguu     1740

accggcaagg ggcuguuuaa cuaccuuaaa aaggaggacg aagaacuggc auuggaggau     1800

auuucaggau ucgacaucga uuuuaaaagu ucccucacua gcuaucugga cuucaagaag     1860

cagauuuuag gcgaggagau cgaaaaagau aaguacaaag guauagugga gaauauuauc     1920

aaguggaaaa caauuuacga cgaugacucu aagaugauga agaaaaugau cgagagagaa     1980

uaucccaacg ucuuuucaaa ggauaaaaua aagaaaaucu gcagguucaa guacaguggg     2040

uggggcaauu ucagccuguc cuuucuaaac ggcauucgug gagcugaccg ggagaccggg     2100

gaaucuuuca cgaucauuga ggcccucugg aaaaccaauu auaaccugau gcagcuuuug     2160

ucgaagcaau uuacauuccg agaagagauc gaucugauaa augcugagaa ggugggaaaa     2220

auuaacaagg uaagcuacga caauacuguu aaagaccuca ucgugucccc ugccaacaag     2280

agagcaaucu ggcagaccgu ccagauuaca gaagagauca aaaaggugau gaagugcgaa     2340

ccagagagga uuuuuauaga aauggcccgc gguggcgaga aagagaagaa acggacuguc     2400

ucaagaaagg cuaggcuguu agaacuguau gcggcaugua aagaugacgu gcgcgauugg     2460

accaaggaga ucgaagaccg agaggaacgg gaguucaaua guaagaaacu uuacuuguau     2520

uacacacaga uggggcguug cauguacucu ggagaggaaa uuaacaucga ugagcucaug     2580

caaaagaaua gcaaauggga cauugaucac aucuaucccc aguccaagau aaaagacgau     2640

ucacuggaca acaugguucu agccaagaag gugaucaaca auagcaaaca gaacaagcug     2700

cucucuuccg auauuagaau caaaaugaag aaauacugga augcucugua uaagggcaac     2760

uuuauuagua agaaaaagua cgacaggcuu acccgcacug aagauuucac gaaugaggag     2820

uugagcgguu uuaucgaacg gcagauagua gagacaagac aaucuuccaa agccgucgca     2880

gaacuguuaa acaggcucua ugagcacuca gaaauugugu acguuaaggc cgggcuggug     2940

agugacuucc gacaugacaa uaaacuucug aaaagccgcc ggaucaacga uuaucaccau     3000

gcuaaagacg ccuuuuugaa uaucgucgug ggcaacguau acaaugcaaa guucaccucg     3060

aacccuagaa aguggauuaa agagaauagg ggaacuaacu acuccauacg ucgcguguuu     3120

gauuauaaug uuaagcgggg caacaccguc guguggcagg ggguggagga cggaggucac     3180

ucuaucgaug aaauuagaaa aacaauggcg aggaacgaca uccucuacac cgaguauacu     3240

uacugugaua agggcgaacu guucaaugcu acacuaauua aaaaggggaa cgacaaggcc     3300

aucaaucuga aaaaggcacu cgauagcguc aaauauggag gcuacaacuc accaaagacg     3360

agugccuucg cuuuuauaga guucgacggu aaaaagaagg aacggaaaaa ucauaucguu     3420

gagauucccg uguauaucgu gaacauugcc aagcgacagc cggauaccau caaagaguac     3480

cuggaacaga agaaagggua caagaaugua gucgugaaga aauauccuau aaagaaaaac     3540

gcacuuauua agaucgacgg cuacuuggcu cgccugagag gagccaauga gacugacauc     3600

uuacucaaaa acgcgguuca acugauucuu aaugauaagu auuacgaaau cauaaggcgg     3660

guggagaaau aucuggaaua caacguccga uaugaggccg aguccauguu ugaaggcuuc     3720

gaccacgcug aucuaaauuc ucucuacgac gagcugacaa gcaaguugga aaccuccauu     3780

uacaagaaaa gacccgcaaa ccagcugaau gccuuagaga aggggaaaga gaaguuuaaa     3840

aacauccaga cacuccgcga aaaggcucaa auuaucaagg agaugcugau aauguauagg     3900

ugcgaugccg caacuguggc gaaucuuaaa gccauuggag gcucagcuaa cgccgguagc     3960

augaagauca aaaagaacac ccugacgagu cguucuuugg uucucgugaa ucaguccauc     4020

accgggcugu ucgaaacaga ggaggagcua guauga                               4056


<210>  3028
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3028
auguacaaga aauauggcau cgggcuggac auuggaacca gcuccauagg uuggucugug       60

gucgaugagu acggccacau cauuagaguu aaggggcaga caggacucgg cgugaggcuu      120

uuccgggaag gucaacccgc cgcugaccgc cgaacuuuuc guacgaccag aaggcggcug      180

gggcgcagaa gguggcgguu gcgcuuacug cgagagaucu ucgauccuua uauugcagcc      240

caggacccaa acuuuuucgc gagacagaaa gcuagugcca ucgcaccggg agauaguagg      300

uuuaaggccg uauaccggcu auucaaugac cagacagauc gucaauuuua ugacaaauac      360

cccacugugu aucaucuccg ccagaagcug augaccgaaa agagacaguu cgauauaagg      420

gagaucuacc uugcugucca ccauauugug aaauaccggg gccacuuucu gacaggcggg      480

gcagccaaga acuucaaacc uggaaaguug gaccucaaua ccuauuuugc uacuaucaac      540

gcccagcugg cggcaauuuu cauggauagc caaguugaac ugccacagcu cgacugggau      600

gccguggucu ccuuacugac aaaugaccag gagucucccu cggaccgaca gaaagcucuu      660

accaagaugu ugguggccca aggugaugca acgcagaagc gcuuuuacac ugaguucuca      720

aaagcucugc uaggccucaa ggccaaacug uauguacuua ccggguucga aggagacaag      780

acagaucaga aagcgcugac uuugagccuc gagacccugg acgaucauca agacgauguu      840

acacaguuac ugggcgacga acagagacag cucguggagc uucuggccag gguccaaagu      900

gcaauccagu uggcuauaau uaucccuaac gguaagaccu uuuccgggca gauggugcag      960

caauaccaga cgcacuauga ucagcuacgg cugcucaaag acuaccgcga gcagcugacu     1020

gaucaaaagc acagaagggc ccuuaagcag gcuuaugccg acuaccuggc aaaugaccag     1080

gaucaggacg ccuuggugaa agcugucgaa aagguucuca aacaaaacca gccacugucu     1140

acagugggag uacguuuaca gaccgcgauu gaugcaggcg acuuccugcc caagcaacgg     1200

acuaaagcca auagcgugau cccucaucag auccaccagc aggagcuaga ucgaauuaua     1260

gaagcucaau ccgucuacua uccauggcuc gccgaggaaa acccgguggc agcccauaga     1320

ucauacgcuc ccuauaagcu ggacgagcuu guugauuuua gggugccuua cuaugucggc     1380

ccauuggugc agccccaggc cgacaaggca gcgaaagaga caaaguucgc uuggaugacc     1440

cgcaaagaag cccagaguac gaccccuauc acaccaugga auuuugagca agaaguagau     1500

cggaacgcaa gcgccaaugc uuucauucag agaaugaaga cuaccgacac auaccugcuc     1560

ggggaggaug uucugcccaa aaacucucuu cuguaucaga gguuugaagu guugaaugag     1620

cucaacaacg uccgcgugga cggaaagcug uuaacuaucg ccgagaagca gcgacuguuc     1680

caagaucuau uugucgaccg gucccaggca ucagugacca uagcucagcu ccaagccaau     1740

cugauugcgc acggugccgc uaaagaccgu guugaaauca agggccuugc agauccgaaa     1800

agauucuuga gcagucugac aaccuaccac gacuacgccc agaucauucc ugaggcucuc     1860

gaacagcccg auaagagggc cgacaucgag aaaauuauaa acuggucuac uaucuuugaa     1920

gauacgucca uuuucaagga gaagcugcag gagaucgcau gguuaacacc agaccaacgc     1980

cggcagcuga gccagaaaag auauaggggg uggggacagc uuucggauaa guugcucacc     2040

caacugcgag acgcgacugg ccagucugug auggaucuac uguggaccga accuaauaac     2100

uuuaugcaga ucauucgccg gacagaguuc cagcaagcca uagcuagagu aaaucagacc     2160

gaccucaaac agcaggaucu ggccacuaca aucaacgacc uuuacacguc accccaaaau     2220

aagaaagcaa uuaggcaggu cuugcuggug guugcugaua uccagaccgc caugcaugaa     2280

guggcaccau caugguuauu cauugaggcc gcucgugguc ccgagcaaaa cccuaagcgc     2340

aaacucgcgc ggcagcagca gcugcuugaa gccuauaaga cuucagcaaa ggagaucguc     2400

aaugaagcug ugaaagagaa gcuguugagc gccauagacg accaccaaaa auuugaagau     2460

cagguagugc ucuacuucca gcagaacggg cuggacaugu auacaggcaa gccacuagau     2520

uuugagagac uguccaaaua ccauaucgac cacauuaucc cgcaaucugu ugucaaggau     2580

gacagccucg auaauagggu gcugaccaca cccgaggaaa acgagcgaaa gggagcccgg     2640

gugccuggcc cacuuuaugc uucacagcag gggcgcugga gauugcugca gagggcagga     2700

uuaauuaguc aacggaaacu cgaccaucug cuucugcgac ccgaggaacu agaucagcag     2760

gccacugguu ucgucgcgag acaacucguu gaaacccgcc aggugaucaa gcugauaacg     2820

aauauuuugu cccagcagua cgacccugag caaaccaaac uggugagcgu aaaggcuggc     2880

uuaucucacc aguuuaggac acgucucaac cugccaaaac uucgggagcu gaaugauuac     2940

caccaugccc acgacgcaua uuuggccgcu agaaucggga cuuaccuccu gcagagguau     3000

cccaaccuag aacgcuucuu ugucuacgga caguaucaaa agacccgauu cgaccugaag     3060

agacauuuua acuucauugg cgagaugcuu aaagguaagc cggaagaugc agugaucguu     3120

aaagccccuc acucagagac agugauaugg gacaagcagg augaagucaa acagcucaag     3180

aagaucaugg aguaccggca ccuguuggug acucaugagg uuuaugacaa ucacggggcg     3240

cuguuuaacc agaccauuca uccagcuaaa aaucccaaga acaggaaauu aaucccucgc     3300

aagacgacac aaaguaccga acucuacggc ggauacagcg gcgagacuac aggguaucug     3360

gccauugcac guaccgugac uaaaaauaag aucuccuacc agguacuuag aguccccacc     3420

cggcuggugg gaagguugca gcgaucuucg gaaagcgccc agcgccaaau acucacagau     3480

ugguucgcuc cauccuuugu gucaagaggu aaacagcagc cuuucgagau uguccugcgg     3540

cacguuuuca agaaccaaac ggugaucgac gauauccaug agcagaguca cagguuuggc     3600

cuacgcucug acaccuauua cugcaaucag cagcaacugg uacuuagccu cgaacagcag     3660

agaaugauua ucgaucagaa gcugaacaau acuaacaaau ugaugguggu cuaugaggcc     3720

gugugugacc aaguugcaaa guacuucccc uuauauagga ugaaucacuu uaaagaaucc     3780

cugcucgagg cugggccaaa gcuggccgaa cuuccgauua acacagugca guccaaaaau     3840

ggaaagcuga aguugggcca gcggagcguc cuacugcaga ccaugcaagg ucuccaugcg     3900

aacccugaga caaaagaucu gucuauaauc gggugcccca aguacuucgg caaacuucag     3960

guguccagug gaauuaaguu aacugagaau gccguucuga uccacgaaag cccaaccggc     4020

cucuuucagc gucgcgugcg auugucugac cuguga                               4056


<210>  3029
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3029
augaagaucc ugggccucga ccuugggacc aacagcauug gaugggccgu ggucaaugag       60

gcugaaaacg agagagaauc cucuucaaua aucaggcugg guguucgggu gaaucccuug      120

acaguagaug agcagaacaa uuucgaaaaa ggcaagccua uuacuacgaa cgcagaccgc      180

accuuaaaac gaaguaugcg uagaaaucug caaagguaca agcuacggcg cgccaaccuc      240

gugagccugc uuaaaacaca gggguggauc acugaugaga cccuguuguc cgagcaggga      300

aauaacucua cauuugaaac cuauagacuc agggcggagg cugccacuaa ggaaauuaca      360

cuggagcagu ucgcacgggu ccugcucaug uuaaauaaga aacgcggcua caagucguca      420

cgaaaaauga agcacgacga ggaugguagc uuugugaaca aucuggacau cgccagagaa      480

cuuuaugaua guggguugac cccaggacaa uacgcuuaua accugcuaaa agacggcaag      540

aaguacauac cggauuucua uagguccgac cuccaggagg aacuggauaa aaucugggca      600

uaccaguacg aguaucauga aaauauucuu acgcacucuc ugcggagcgc caucauugac      660

caguccaaga cuggcaccac aaaaaucuuu uugaagacuc augggauauu caccgcugau      720

auuaaaggaa accguaacga gcgcaauaag caacucuuug agcugagaug cggugccuua      780

gaaagggcgc ugacagcaga ggaacucgcc accguuaucg cugaggugaa cggccagauu      840

aauucaagua gcggguaccu uggagccauc ucugaccggu ccaaagcacu guucaucaac      900

caccagacgg ucggccagua uuugaugcaa cagcuagacc gagaucccaa uaagucacug      960

aagaaccagc cuuuuuaccg ccaagacuau cucgaugaau uugagcagcu gugggagacu     1020

caggcucauu accacccaga acuuacagcc gcgcugaaac acgagauuag agacauaauc     1080

auuuucuauc agaggcggcu aaagagccaa aaauggcucg uggccaauug ucccuucgaa     1140

caucgcaagg uaugcccuaa aaguucucca cuguuucagc acuucagaau cuggcagguu     1200

uuaaacaaug ugaccgucac uguggauaac aagacauacg ugcugaauga ggaacagaag     1260

uccauacuaa gcaaagagcu ccuguacaag gagaaaauuu cgaagaggga cgcacuuaaa     1320

auguuguucg ucaaggcuaa ggguguugau augaacuuug ccucucugga aggggaccgu     1380

accaugucca aucuccugaa agcugcccaa gagaucgugu caaucagugg acauggcgau     1440

uaugacuucg gcaagaugcc cgcagaugac auucacaaca ucauucggag cguauuuggg     1500

ggacuugguu ucgccacgga cuggcugaau uuggauaccg acgcugauga ccucgaacag     1560

gagucccaga ugcgacugug gcacuuaaua uacucuuuug agggcgauaa cagcacaacu     1620

ggggacacca aacuggugga aagacuaaug ucacuccuga accuuccuca ggaguaugcg     1680

agguuguucg caaaugucca ucuggaagau gacuacugug gaaucaguuc caaggcccuc     1740

aaacgcauuc ugccauuuau gaaggcuggc cacaucuaua gcgaggccug cgcauuagcc     1800

gguuacaagc auucugaaca aucacugaca cgggaggaga acaaaaauag accgcuuaag     1860

gaucacuugg aacagcuccc caaaaacagu cugaggaauc cugugguuga gaagauccua     1920

aaccagaugg ugaaugucgu gaacagcauu auagacacuu augggcgccc agaugaaauc     1980

cgaauugagc uggcucggga acucaaaaau aacgcccugg gccgucaggc aaucuucgag     2040

gcgauuaaua gaaacaccaa ggaguccgaa aggauccaac agauacuuaa aaaggaguuu     2100

ggauugacac auguaucucg caaugacauc auucgguaca gacuguacca ggaauuaucg     2160

ggcaacgggu auaagacccu cuacagcaau aucuauauuc cccaagagaa acuguucucc     2220

aaggaaaucg auauagagca cauuaucccu caggcuaggc uuuuugacga cucauucagu     2280

aacaaaacuc uggaguaccg agauguuaac auuaagaaau cuaauacgac agccaucgac     2340

uuugugcgca acgaauaugg agagaaugaa gucgagcagu acaagaagcg ggugaacgau     2400

uugcucaaaa gcggugcaau aucccaggcc aaggcuaaaa agcugaaaau gaccgaggcc     2460

gaaaucccaa augacuucau ugagagagau cuaaggaaca cucaauauau cucacguaag     2520

gcacaggaaa ugcugcgcga cgucgcucgg gugguuaccg ccacaaccgg cagcgugacu     2580

gauagacuca ggcaggacug gcagcuggua gaugucaugc aagagcuuaa uugggacaag     2640

uacgaacgac agggguugac aacggugauu aacggacggg auggccagca gauccgcaga     2700

auaaccgacu ggacuaaaag gaaugaucac cggcaucacg cgauggacgc ccugacaauu     2760

gcuuucacca agcccgcaua caucucuuau uuaaaccauc ucaaugcccg auccgaggac     2820

gauaucagaa guauugagcg cagguuucug gaacgugacg cuaacgguca ccuucgguuc     2880

aucagcccga uugcccacaa uuuuagaucu gaggcaaaaa ggcaucugga aaugauauug     2940

guuuccauca aggcgaaaaa caaggugguc acacgcaaua acaacacuac caagacgaaa     3000

gggggccgaa gaaaucggau ucaacucacc ccuaggggac agcugcacga ugccacagug     3060

uacggcucaa ucuugcugcc ccauacuaag accauugcug uagacgggaa aaugacagcc     3120

gagaucauag gacaggugca aaacccaguu uuccgccagg cauuaaguca gcgucucgag     3180

gcuuguggua augauccuaa gaaagccuuu acuggcaaga acagccugga aaaagcaccc     3240

gucuggaucg accaggccca caccgagcau gugccagcug aagugacgau ucaaagacuu     3300

gagccccagc acacaauccg gagggcgguc aauaaggauc ugaacauuaa uaagguuuug     3360

gacaaaggga uccagcacau acuccaggcc cgccuggaug cauauggagg cgacgcugcc     3420

aaggccuucg cuaaccuaga uaauaacccu auuuggcuga aucaagaaaa agguaucgca     3480

aucaagcgag ugaccacugc cgagaacauu ucgggggaac cacuucauca caaacaugac     3540

aaggagggca gaccgauacu cgacacaaau ggaaagccca ugccuuccga uuuugugcgg     3600

cugaugaaca aucaccaugu agcgaucuuc caggacgcug auggcaacuu gcaggagcac     3660

auugucucuu uuuacgaagc cguggcaagg guuaccgagc ugggguuacc agugaucgac     3720

aaagaauaua auaaggauaa aggauggcag uuccucuuua cucugaagca aaacgaguac     3780

uucgucuuuc ccaacccugc ccagggcuuc aauccagacg agauugaucu uaccgacgaa     3840

cgcaacuaua gcagaaucuc acccaaucug uacagggugc agaaaaugag uuccaaguac     3900

uauguuuucc ggcaccauuu ggagacaacg guggaagaua agaaagagcu ccaggguaua     3960

accuggaagc guauccaauc ucugaacaau cuaaaaggga uuguaaaggu ccgcaucgac     4020

cacauuggac gaaucgugca gauaggcgaa uacuga                               4056


<210>  3030
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3030
auguucagcc agcaaaacaa ucuguacugc gagucccucu cuaaguugua uuacaacauc       60

accaaaaauu uucugucaaa gaacgaagug uguagaauuu augagauauu cuuuugcagu      120

aucuugacau acuucuuaaa gaaguauaau cacgacauuu uuaaaaagag cuccacuaug      180

aagacgaucc ugggccuaga ucucgggguc accucuauug gaugggccgu uaucgugaca      240

gacgaugaca acacucccau aaaaauccug gguaugggcu cgaggauuau cccucuuuca      300

accgcugaua aggaagaguu ccagaaaggg caggcaauua gcaagaauca gagucggaca      360

aucgcccgca cccaacgaaa aggauacaac cguaagcagc ugagaaagga agaguugaaa      420

aagauacucg gcgagcuggg uauuacucca uccaaagaac ugcucaauuu accgucuauc      480

gagcugugga aacuuagggc ggacgcugcc agcgggaacg cacccuuguc cgccgaacag      540

cugggacgga ucuuuuauau gcuaaaucag aaacgcggcu acaagucagc uagaagugag      600

gcaaaccaag auaaaaagga cacagauuac guagccgcug ugaagggcag gcaucagcag      660

cucaaagaga aggggcagac cauuggacaa cacuucuaua cggaaaugcu gacucuugag      720

gaaaauagcg gucauuuuca ggucaaaaac aauguguacc cucgggaggc cuaucuggaa      780

gaguucaacg cgaucauugc agcccagaag ccacaacacg cuuuucuuac cgacgauguu      840

guggaccgcc uccgagauga ggucauauuc uaccagagaa aacugaaguc ucagaagggc      900

uuagugucca ucugugaauu ugagggguuc gaaaggacau ucuuugacaa cguaaaacag      960

aaggacaaaa cuauuuucac cggacccaag guugccccua aaucaagccc acuguuucaa     1020

cucugcaaga ucugggagac agugaauaac auaaaucuua aggucaaaaa caccgauggc     1080

aguaagaaua aaugggcaga cuaugugccc acgcuggaac agaagcagga gauuaucgag     1140

cauuuguuca aaaacgcugg uaucucucua gaugcccugc ggaagauucu cggguuuaau     1200

aaggacgaaa ucuucgugaa caaacagauu gagaagggac ugcaaggcaa uacuacauac     1260

gcggaaauac acaaaauccu uggcgauucc aagccugagc uguugggguu uaacauuaau     1320

aucgaaccaa gcaaacacuc ggcccuccug gucgacaagc guaccggaga gaucuuagca     1380

gagaaagaag gucagcaggu uucugcugag auugaaaagg agaacuucua ucagcugugg     1440

cauacuauau acuccaucaa ggaucuagac gagugugccg cugcccucau uaaacgcuuu     1500

aauaucgauc cgaacguggc agcccaacug gcuaagauug acuucucaaa aggcgcguuu     1560

gggaauaaga guaacaaagc aaugagaaag auccuucccu acuugaugca gggauauaac     1620

uacgccgaug cuugcagccu ggccggcuau aaucacuccg acucucagac aaagaaagaa     1680

aaggaggaaa acguagugga cgauaaacuc aagcugcuug agaaaaauag ccugaggcag     1740

ccugucgugg aaaagauaau caaccaaaug auucauguug ugaaugcaau cauugacaag     1800

uacgguaaac cagaugagau ccgggucgag uuggcccgag accucaagaa aucaaaggau     1860

gaacgcaacg aggcugaacu gcagaaugcc aguaacaaaa aguuaaauga ggaaguggca     1920

aacagacuga aggagcuagg gcuccccgua accaaaaggu ucguucagaa guauaaauuu     1980

auauucccug ugcgggaccg caagauuaaa gaggcgcaac uggucaauca guguaucuac     2040

ugcggcgaaa cguucaacgu gaccgaggcu cuuuccggag auaauuuuga cgucgaucac     2100

auuaucccaa agagcauguu guucgacgau ucucagacaa acaaagugcu gguucacaga     2160

aaguguaaug ccaaggacaa aucaaacaag acugcauaug auuuuauagc cgcuaaaggc     2220

gaaaacgagc ucgccaucua ccuggacagg guggacgauu gguucaagcg ugggguauua     2280

aguuacggaa aaaugcagcg gcugaagguc agcuaugaac aauacgcaga gcgaaagcag     2340

cuuaaaaagg agaccgaagc ugacauuaaa uugugggaga auuuuaucga aagagaucuc     2400

aggcagacac aguauauugc caagaaaucc aaggaccugc uaaagaaaau cugcaacaau     2460

gugacuguua ccgaggguaa gauaacagcg caacugcgca accucugggg cugggaugac     2520

gugcugauga aucuucaguu gcccaaauac aaggcccugg ggcagaccga gauuaaagaa     2580

uggacuucug agcauggacg gagauuacac aagaaggaag agaucacgaa cuggacaaaa     2640

agggaugacc aucgccacca ugcuaucgau gcacucgucg uggccuguac caagcagggc     2700

uucauucaac gaaucaauac ucugaacucg agcgacacca aagaugcuau ggaccgggaa     2760

auuaaggccg caaaagagaa guauaaugag cuuuacgaua agaaacuguu gacagaaacc     2820

aacgagcagg uaaagaauca cacugaacgu uuuaacaaac ucgagacaua ucugaagucc     2880

cagcaaccgc cucuauucac gaccagauac guggaacagg aggcggacaa aauauuuguu     2940

ucauucaagg ccgguaaaaa ggcugccacu uacgggauca ggaagauuaa aaaggacggc     3000

aaaaaggucg uggugcagga gaaaaucguc guuccccgcg gagcacugca cgaacagagu     3060

guguauggca agauuaagac acaagagaaa gauaagccac ucaaauaccu guuugaaaau     3120

ccugagacca ucguggacaa ccggauaaag auccuuguaa aagaaagauu ggauaaguuc     3180

gagggggacg ucaagaaagc ucugacaucu uuaaagaaag agccccucuu uauugauaau     3240

gaaaagaaca cucugcuuga gaaagccgca ugcuauaccg acgccacggu gcugaaguac     3300

aagcuacagg cucucaaagc gaaccagguu aaugauaucg uggacgaagg agucaagagg     3360

auugugaaag cccgacuggc acagcauaag aacgagaaag aggcuuucaa ggauaccguu     3420

ugguucaauc aagaaaaacg caucccaaua gugagcguac gggucuuugc cagacccgac     3480

gccuccaagu uggcuauuau caagacagau gagaacaaua aaccuaucgg uuucgcacug     3540

acuggcaaca aucaccauau ugccauauau aaggacaaca ccgggaaauu agugcagcac     3600

aucugcacau uuuggucagc gguggaaagg aagaaauucg gagucccaac uguugugaag     3660

aauaccggca cgcucuggag cgagcugcuu cacaaggacc ugccggguac auuuuuggau     3720

aaacuccccc aggacgggcu ggaacuagag uacucucugc agcaaaacga gauguucgua     3780

cuuggccucc cuaaggaauc cuuugaggau gcuauuaaaa auaacgaccu gccauugcug     3840

aguaagcauu uauaucucgu guggagcauc ucugauaaug acuacugguu ccgucaccau     3900

cuggaaacca aaaacuccga gcuuaaguca acuacaggag ccaaggaaag uaaacgcuac     3960

uaucgguuuc agagcgucgg caccuucacu aaggagaauc ccauuaaagu gcagcugaac     4020

cacuuggggg agaucaccaa gauaggagaa ccuuga                               4056


<210>  3031
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3031
augaagaaaa gaaacaggga ggaauacuau gugggccugg acaucgggac cagcuccguc       60

ggaugggccg uugcuacacc cgauuaccgg auuccuaagu uccgcgguca caaaaugugg      120

ggcucucgac ucuuugcaga ggggaagcgu gccgcggaaa gaaggcaguu ccggacuaau      180

cgcagaaggc uugcucggcg ccgagacaga cugcaaauau uggaggaauu auuugccgag      240

gagauggcaa aaguggauaa cacguucuuu augaggcuga aggaaucaaa guucuuucua      300

gaggacaaaa ccguaaagga aaaacauauc cucuucagua cagauaaguu ugacgccgau      360

cgguucuaua aagacaaucc aacuauuuac cacgugcgua agcgccugau ggaggaggaa      420

ccgcaggaua ucagagagcu uuuucuggcu guccaucaca uuuugaagua uaggggacau      480

uuccucuuug caggcagccu ggaacagaac ggugccucca ucgaggaacu ggugcggacc      540

cucuuaggga aacagggacu ggcuauacuu gacgauacaa ucgacucuaa ugagaugguu      600

aacauuuuga agaauaccgg cgagacuaaa ucggaucgaa agaaagacgu gcaagaacug      660

uggaaccccc uaaaggacaa agucaagaag gcccaggcgg cagagauggc cggccucgug      720

cuggggcuua aaucagauau cagcaagcug uucgcuaaua gugacuacaa agauuuggac      780

uccucuauua agaacaucaa uuucagcucc ggagauuaug aagagaaacg cgacgauuac      840

gccaacucaa uaggugacga uauuguacuc cuggacacaa ugaaggcauu auacgauacc      900

cugauccuca gucagauucu ucagggcaag agaagcaucu cugcugccaa aguugaagac      960

uauaauacgc acaaggagca acugagggcg uugaaagcca uccuaaagcc ucacaaacgg     1020

cuguacaacg agauguuucg cgaaaauaac aagaaggacc ucuccaacua uguggcauac     1080

auuucaagcu auagugaggg gaaaagaguc aauagggaag gauucuacaa guaugugcag     1140

gcugugcuga aacaggucga ugacucugau uccaagcagg ccauacuuga ggcuauccgu     1200

cuggaaacuu uucuaccacu ccugcggguu aaagacaacg ccgugauucc ccaucaaauc     1260

caccaggcag aguuagaggc cauacuggaa accgcucgaa agaaguacaa uuuccuaaac     1320

gauguaagcg acggcguguc ggucaaagau aagauuaucc agcucuuuac auucagaauc     1380

ccuuacuaug uggguccacu gaauccccau ucuggggaga cuguugcgga caggaaacaa     1440

ggaaccggcu gggugguccg caagcagcgg ggcaaagugu acccuuggaa cuuugcagau     1500

augguagacg uuaaggccuc cgcugaagcc uucauuacaa aucuuaccaa caaaugcacg     1560

uauuugaucg gggaggaugu gcugccaaag agaucacucc uguacaguga guuuaugcuu     1620

cugaaugaau ugaacaugau uaaguaugac ggacacgagc ucccggucgc aauaaaacag     1680

cuguacaucg aacagguguu caagcaagag cacaaaaagc agacuaaagg uagggucuua     1740

gauuuucuga agcgcaaugg ccuaauuaca gggagcgcca ccgcugugau cacuggaaug     1800

gacgacgcca ucaagggcga ucuccagucc uaucgagaca ugguucggau uuucggugca     1860

ggguuugauc auucuauggc ggaacguaua aucagauggg ugacacuguu cggcgaggcu     1920

aaagacauuc uugagaacaa gaucagggaa guauacggaa auagcauuuc agauagucag     1980

augcgcgcca ucaaaaaguu gaaauacgca gacuggggcc ggcuguccca agccuuccuc     2040

auagcuguca agggguguga uaagagcgga guggagagaa acaucauuac cugccugagg     2100

gccucuucac agaauuuaau ggaaauccug agugaccagg guacguauau uaaacagauc     2160

aaggcaugga accaccaaca uaccacaaac augauggaua cucuugacua ccaguugguu     2220

gaugaccucu auguggcucc cagcgucaaa cgauccauau ggcagucucu guguauugug     2280

gaggaauugg uaaagaucau gggccaggcc ccuaaaaaga uuuuugugga gguuacccgc     2340

acaaagcaag cggagaaaac uaagaaagau ucgcggaaga aaagacuggu cgaacucuac     2400

accgccaucg ggaaggaggg agcugcaugg aaaaaggaaa uagaggccca ccaggcuggc     2460

gacuucaggc guaagaaacu guaucuuuac uauacacaga ugggucgcug cauguacagc     2520

aaugaaccaa ucgccaugga cgcauuguuu accuccgcgu acgauauuga ccauaucuau     2580

ccccaaucac ugacuaagga ugggaguugg gacaacuuag ugcucgugaa aucucagcug     2640

aauaaggaga aaagcgauca guacccucuu cggccagcca uuagagagaa gaacaaggcu     2700

cacuggcagu aucuguguag ggccggcuuc aucuccgaaa agaaguacga gcgauugaug     2760

cggacgacag aacucaccgc agaggagcug ucaggauuua uagcucgcca gcuagucgaa     2820

acuagccagg ccaccaaagc aguuacaggc cugcucgaga gaauuuauac caauacugaa     2880

aucugcuucg ugaaggccga gacagugucu caguuuaggc accagcauaa cuucaucaaa     2940

auucgggaac ugaauaacua ccaccaugcu aaggacgcgu aucuuaauau cguagucggg     3000

aacguguacc acgagaaguu uacgaaaaau cccugggagu ucauugcccg auccaccgaa     3060

agacgcccgu acaacuugaa uaggauguau gauuuugacc uguuaaagca aggacguguu     3120

auauggcaag ugccuaaaag ucucgagacu guccaggcaa ugaugaaaag caacgaugug     3180

cggaucacua aaaagcugcu uguucagaag ggugaacugu acgacgcuac cauuacaaaa     3240

gcccacaagg ccaaagaggg cacuuauuuc ccccuaaagg gggcugaugc aagacucagg     3300

gacgugcauc gcuacggagg cuaugagaaa aucaagauug ccuacuauuc uguauuuucc     3360

ugucgagaaa aggcgucaaa uaccauccac acgcugauaa ucccaauucc ugucuacaaa     3420

gcugguuugc ugcaaaccga ggccgacuua acagcauacg ccuggcagac uaagcagaaa     3480

accaagccca ucaaagauau uuauagugcu aucacaccaa uauacaagaa acucugcauu     3540

aacacugugg ugaagaucga cggguucaag uauuuccugg gcggaaaaac cggcgauuac     3600

auguggcuug accccgcaau cccugucguu cuggaugaac aagccgcggc uuauuugaag     3660

gccauuagca aaguggggag agcagacgau guagagaaca cggcccaggc uauagaccuc     3720

aucacagccg aagcaaauga ggcuuuuuac gaugugcugc uaaagaaaau ggaagccaag     3780

auuuucgcga aggccuauca gaccaucguc gagaaacugg caucggagac ugcucgggaa     3840

agguuucgcg agcuuuccau uggagcccag uguucuugcc uccuggaaau cuugaaccug     3900

uuagcuaaua acaagacaac cuucacauuu uucaaagaca agaugggcgu gaccaugagc     3960

cguagaaaac aaccaaugga ucucacaaag cuggaggagu guauaguuua cgaaacguca     4020

aucaccgguc uuuacgagaa ggcagugagu cuguuguga                            4059


<210>  3032
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3032
augggcaaga gauaccacau cgggcuggac auuggaacca gcuccauagg uugggccgug       60

auggaugacg auuucaaaau caugaggguc aagggcaaaa aggggauugg aguucggcuc      120

uuugaggaag gcaaaacagc ugcagagcgc cgaggucauc guacuacgag aaggcgguau      180

ucucgcagaa aguggaggcu uaaccuguug gaagagaucu ucgaccccga aauugccaag      240

guggauccua ccuuuuucgc gcgguuaaaa gagucaaauc ugaguccaaa ggacgaucgc      300

aaacaguaca gcgggaaccu aaucuuuccg gagaaggcua aagaagagau acgagaaugg      360

acagagaagu aucccacuau cuaccaccuc agaaaugccc ugaugaccga gaacaggcaa      420

uucgacauuc gggaaguauu ucuugcacug caucacaucg ugaaguaucg uggaaauuuc      480

uuguuuaacg gcaauuucca gacaggcgag gucaaccucu ccgaucgcuu ugacgauauu      540

aauucugccu ucaucucggc uugcggggaa gaggaagcac cucagauacc aucagagcug      600

uacaacgaca uucaggagcu gcucuuugau aaaaauacca gaaaguuaga caaccaaaaa      660

cagcuggcca cuaaacuuag ccaggacaau gauaaagacu ggaguacaca guucuccaag      720

gcuuuguucg gauauaaaac caaguuuaag gugguucugc aaucuaacga agaucuagac      780

gauuuccagc ucgacagcga ugacgccgau gacaaaaucg cguccauuuc agagcagauc      840

gaugacgaca gggguaguau ccuggauaau auuaagauac uuuacagccg gaucacgcug      900

aacgaaauug ugcccgacgg caaaacuuug ucucagucca ugaucgagaa guacaaccuc      960

caucgagauc accugaaaga aauaaaggac caauuaaagc acaccgauaa uugggucaaa     1020

uuugacgauc uguauucaca guacgugaac gggaagcucg agagcgaccg ccauaaaaag     1080

cugaguucug augacuucua uaaaaaggua cuuacauugu uugauggaga gcuaaagacu     1140

agaguuaaag aauggcagga gaagggcgaa uuucugccua aacaaaggac cgguuccaau     1200

ggggugauuc cacaccagcu ccaucagaac gagcuggacc agaucaucga aaaucaaagc     1260

aaguacuauc ccuggcuugc aacacugaac ccuaaugaga aacggcagcg cgucgccaag     1320

uacaaguugu cggagcucgu ggcuuuuagg auuccauauu acgugggacc gauggucacc     1380

aaagaucagu cugacuucgc cuggaugguu agaaaguccg acgaaaaaga gaucacgccc     1440

uggaacuuug aucagguggu agaccgugau aagucagcag aagaguucau uaaucggaug     1500

acuacaaaag acaccuaccu gauaggcgag gauguguuac cugcuaagag ucugcuauau     1560

gaaaaauuua cuguccucaa cgagcugaau aacauccgaa uuaauggcaa gccacuuaca     1620

accgacgaaa agcaccaagu guacagagau uuguuccaga acgccaaaag caucuccacg     1680

aagaaauuuc acaauuaucu gguuuguaag cucaacaaac ccaaccaucu gaauaucacc     1740

ggguucucug accuuacaaa guucaacagc ucacuggaua guuacauuga cuugaagaaa     1800

auauuugcgg aucagaucga cgaucagucc cuccaagacg acuucgagaa gaugauuagg     1860

uggagcacug uguuugaaga uaaacagauc uucgagcgca agcugucuga gauuaauugg     1920

uuaaccgacc aggaacgguc agaggucgcc aaacugagau auaggggaug gggucgccua     1980

aguaagcgac uccuggcagg cauccacgau gaaaacgggc agcggauaau cgacgcucuu     2040

uggaauagcc cuaagaacuu uaugcaaauu caggccgagg uggcuuucaa uaaacagauc     2100

aacgauuuga auacaaagga acuguccgac aacaauguag aggauguucu cgccgacgca     2160

uacacuucuc cacaaaacaa aaaggccauu cgucaggugc uguuagucgu ggaggauauc     2220

cagaaagcua ugcauggaga cgcgcccacc ucgauaagcc uggaauuugc aagagaggaa     2280

caggaguccu caaggacacg cagucggaga gccgcucuuu cugccaccua uaggcaaauu     2340

agcgaugaaa ucgucuccgc aacugugaag ggcgaguugg guaaggccac gucaauuagc     2400

gaccgacucu accuguacuu cacacaggga ggccgcgaca uguauaccgg agagccgauc     2460

aauauagaug aaaucucucg gggcuacgag auugaccaca uccuaccuca guccuuuauu     2520

aaagaugaca gucuggauaa cagaguucuc gugaaggggg cuaucaauaa cggaaaaagc     2580

gccaacuaug uaauggacca gucuugggca cccgauaggc uggcguggug gaagaaacuu     2640

uuggacaauc gucugauauc ccgcaagaaa uaccaaucau uaacucucac cccagguaag     2700

auuaacaagu auagucagaa uggcuucauc aaccggcagc uggucgaaac aagacaggug     2760

aucaaacuug uugcuaauau ucugaacgau caauacagca augacgauac caagaucauu     2820

guggucaaag ccgcauugaa cucgcagcuc aggggguuuc uggagcuauu caagaaucga     2880

aacauaaaug acuaucauca cggaauggau gccuaccugu ccacuuuuau cggcaacuac     2940

cucuauaaaa aguacccuaa gcugcggucu uauuucgugu acggugaguu caaaaaguuu     3000

agcgggaaac agaaucuuaa gucauucaac uuuuugcacg accuggaaga cgauaguauu     3060

aauaucauuu ccaacgacga uggcgagauc guaggaaacc aucacgaaau gauagaggaa     3120

uuagagaagg cuuaucaaua caaguauaug cucgugucuc gcgagguuuu cgaaaagaca     3180

ggcgaccugu uuaaugucac gguguacagc agaaaagcca agaaaacccu uauccccauu     3240

aagcaggaua aaccaaucaa cauuuacggg ggauauuccg guaauaacga cgcauacaug     3300

gcuaucguga agcugcccgg caagaaaggg aagccugaua cuuauaaaau agucgggauu     3360

ccacuaaggu ucgccggcga gcucgaacgg gcgaagcagc ugggugaguc agccuaccga     3420

gaaaaauuga gcgcuguuau cacaccgcag uuuaccaagg agaagaaaga cagaaagaca     3480

ggggagauca cuaaaguggu ggaugacuuc gaaguagucc uggaucgcgu gcccuauagg     3540

caauuauuua aggacaguga uaaaugguuc augcucggcu cugcagccua ccaguauaau     3600

gcuaagcagc ugguucuuaa caaugacgac gugaaacugu ugaacgagca gggacguaag     3660

aagcucgaac ugccuaauga gaaacuaucc agcgcccuga acgaugucuu ugaccauauu     3720

auacaaauca ugaacucgaa guucggccuu uacgauaaaa auggguuccg gaccaagcuc     3780

acggaagcag cgucaauuuu cuacgaccug ccauugagag ccgaguauaa uggaaacaaa     3840

aaggugaccg auggcaagcu ggagguuuua gacaauaucc ucauuggucu gcacgcugau     3900

cccucuuucg ggaaccuuaa acagcugaau augaguacac cuuuuggaca gaugcagacu     3960

aagggcggug ugguauugaa cggcaacucc acccucaucu accaaagccc aacagggcug     4020

uucgaaaggc gcgucaaacu aaaggaccug ucuaaauga                            4059


<210>  3033
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3033
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggaugggc cuucauaaac       60

gagggugaaa augauaacga gacuuccucu aucauuaaug cuggcgugag aaucgucccc      120

cugacgaccg acgaagaggc agauuuuaag aaagggaaca caauuucaau caaugccgac      180

aggacuuuga agcggggagc gcgccgaggc uuacagcguu ucaaacaaag aagggaugcu      240

cugcuacaga guuuucgggg uauaaacuuc aucagcucca auuacguuua ugccgaaacc      300

gggaagcaga caaccuuuuc uucguacaag cuccgcgcaa aaucagccac ugagcuggug      360

acaaaggagg aacuuguaca ggugcuguug augcucaaca aaaagagagg auauaaaagc      420

aguaggaagg cuaagaccgc agaggaaggc gacgccauug auggcaugaa aaucgcuaag      480

gagauuuucg agaauaaccu gacgccuggg caaugggucu acaauucccu gucuaucgga      540

gguaaauuua uaccagacuu cuaucggagc gaucuccaga aggaagugga gaaaauuguu      600

cgcuuucaga accaguucca cccggaacaa augaaugaga aguuacugga aaacauccag      660

ggcaaagccc gaacugugac cucccaguac uuuucaagga uucuuaauau ccccuuggcg      720

gagaacaagg ggacaagaga ggaaacuaag cugcagcauu auaaauggcg gaacgaggca      780

cuaaccgagg aacucgaccu gaagguccuu gccuucauca uuacagaaau aaauaaccaa      840

aucaaucaga guaacggaua ccugggcgcu auuagcgauc guucuaaaga guuguacuuu      900

aaucaggaga ccguggguca auuccaguau aaccagcuca aggaaaaugc ccacacgaaa      960

cugaagaacc agguauucua ccgccaagac uauuuagaug aguuugaaaa gaucugggag     1020

acucagucca aauucuaccc ugaacugaau gacgagcuca agucagaggu uagagacaua     1080

acaaucuuuu aucagaggaa acuuaagagc cagaaacauc uggugaguuu cugcgaauuu     1140

gagaaggggc acaaggcaau uccaaaaucu ucccccuugu uccaagaauu ucggaucugg     1200

cagaacauua auaacaucgu cgugaagaau gagaccacua aagagccuuu ccuacuggga     1260

gaagaucuca agaaagugau ugcucaggag cugagcuuua aggaaucgau gacagacucu     1320

cagcuucuga ccuucuguga uuugaagaaa ggcacgcaua ccgucaacuu uaagaaaaua     1380

gagggcaauc gaacaaacac uucccucuuc aaggccuuug agaaaauccu gguuuuagaa     1440

ggguacgacg agcuggaucu aucaaagcuc gacgcgagug auauuaaaag caucaucaag     1500

uccgccuucu cugaccugga aauuagcacc gcaauacuug auuucgaccc aacaauccaa     1560

ggaaacgauu augacaagca gucauacuac caguuguggc aucugcucua uaguuccgag     1620

gaagacgauu uucugaaaaa gagccuuaug gacaaauucg guuuuaagga gucucaucug     1680

cccuucuugc ucaauauuaa ccugcaggcu gauuacggcu cauuaagugc caaagcuauc     1740

aagaaggugc ugccucaccu aauggacggg cacauuuaug auaaagccug cacucucgua     1800

ggauacaauc acagcuccuc ucugaccaca gaccaaaaca augaacgcgu gcuuaaggau     1860

accuuggagc ugcucaaaaa gaacucgcug agaaauccag ucguggagaa aaucuuaaac     1920

cagaugauaa auguuaucaa cgcaauuauc aaggacccgg ccaugggcaa gcccgaugaa     1980

auuagggugg agcuggcucg ggaacuuaaa aauaacaaug agcagcgcag cgaaaugacu     2040

aaggcgauca acaaauccac gacagagcac gagcaaauaa gaaaguugcu ccacaccgaa     2100

uuugguauuc auagggucac ucguaaugac aucauucggu auaaacugug gaaggagugu     2160

gaugggaucu cacuauacac cggcaagccu auagaaccaa guaaacuguu cacaaaggac     2220

uaugauaucg agcacauuau ccccaaaucu cgacucuuug acgacagcuu cuccaacaag     2280

accauuugcg agagacagcu gaacaucgau aaaucaaaua agacugcaua cagcuuuuuc     2340

caggaaaaac uuucugccga ggaauuugac caguucgaga agagggugaa guccuuguuu     2400

ggaaaaauaa gucgcacaaa gcaaaacaaa cuguuaaugg cuaauaacga aauuccugag     2460

ggcuucaucg aucggcagcu cagagagacg caguacaucg ccaagaaagc aaaggaaauu     2520

cugcuugagg uaagcaggaa uguuaccgcc acuaucgggu cugugacaga caagcugcgc     2580

gaugacuggg aauuggucga ugugaugaaa gagcucaacu gggaaaagua ugacaaacug     2640

ggacuaaccu acauugagga ggguaagaau ggcgaacgac uguauaaaau aaaggauugg     2700

acaaagcgga acgaccaucg ucaccaugcu auggaugcca ucacugucgc auucaccaaa     2760

ccagcguaca uucaguaucu caauaaccug aaugcuagaa cgaacaccga caagaaagga     2820

aauuccauca uugggaucga gaacaaguac cuuaaaaggg auaagaauaa caaguugcgc     2880

uuuauagaac ccaucccgaa cuuccgggag guggccaaaa agcaacugga aucaauuuua     2940

aucaguuaua aagcaaagaa uaaaguugug acaaagaaca agaauguaac uaaaaagagc     3000

aacggcacca aucagaaaau ucagcucaca ccuagagguc agcugcacaa ggagacuguc     3060

uacgggaaac uucaacagua cgugaccaag gaggagaaaa ucaacgccaa uuuuacgccc     3120

gaauauguua caaagguggc uaagaaagaa uacagggagg cccugcuaaa gcgacucaua     3180

gagaacgaaa augacccuaa aaaggcauuc accggcaaaa acgcucuguc gaagaaucca     3240

auuuauaucu ccuuggagaa caauauccug guccccgaaa aggugaaaau uguaugguua     3300

aagucugacu acacuauacg caaagauauc acaccagacc ucaagauuga uaaagugauc     3360

gacauuggac ugaagaccau ccuucagaac cggcugaaug aguuuaacgg cgauacuaag     3420

aaagccuucg uuaauuugga cgaaaacccc auauggcaaa acaaggagaa agggaucgcg     3480

auuaagagag ucaccaucag cggaguguca aaugcccagg cucuccacac gaaaaaggau     3540

caucugggua acgagauucu agacgaaaau ggccagccuc ugccagugga uuuugucagu     3600

acagggaaca aucaccaugu ugcaaucuau agggacgaga agggaaaccu ucaggaagag     3660

gugguguccu ucuuugaagc cguaacccgu aauaaccucg gccugucuau aauuaauaaa     3720

aaccacgaga aggguuggga guucuuguuu agcaugaaac aaaaugaguu cuucaucuuu     3780

ccgacugaug gguucaaccc ccacgaaauc gaccuguuaa auacagaaaa cuaccaucuc     3840

auuucccgca auauguuucg gguccagaag aucgcuacca aaaacuauau guucaaccac     3900

caucuggaga caaagucagu ggacggcgaa augcuuaaaa cuaagaagca gcugagcaaa     3960

auaaguuacc acuuuauuca gaccccuucu uacuugaauu auaucauuaa gaucagaaua     4020

aaccaucucg gacaaauugu ucagcugggc gaguacuga                            4059


<210>  3034
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3034
augaagcagg acuacuauau cggccuggau auggggaccg agagcguggg augggccguc       60

acagacguug auuacaaccu cauuaaaaag agaggucaag acuauugggg cguguaccuu      120

uucgaugaag cucagacugc acaggggagg cgggccuuuc gcacgaaucg acguagacug      180

gcgaggguac ggcaccgcuu gaacuuacug caggagcuau ucgacuccga aauaucugcu      240

aaagauuuug gauucuuuua uagacucgaa aauucaaagu acuucgacga agauaaaccc      300

gagaucgcca ggggcaaguu ucugcuuuuc aacgacaagg auuuugguga caaagaguau      360

uacaagaauu acccuaccau uuuccaucug cggaacgcau ugcgcacaca agaaaucaaa      420

gaugugcgac uccuguaucu ggccguccac cauauucuca agaauagagg gcacuuuuua      480

uucggagagc aggacuuuaa cgugaaugac gcuagugaug uuagcggcga guucgagaaa      540

aucaacaggu uccuguccga gaaugacggc gcaggguuug aucgggaaaa gauaccagag      600

aucuucgaaa cucuuugcaa cgagaauuug gccaagcgug aacgcaaaga gaagauucug      660

gagcuagcug gaaacucuaa aaauaagcuc cugggugaag ccgugaacgc ggucgugggc      720

cuuaacuuuu cgcugucaaa aauguucggg gaggacagag auuuucaggg agaaaauaag      780

augagcuuca ccgacaguuc cuuugaggaa aaauuggcaa aggccaggau ggcucucgau      840

gacgaugagg ccgacuuccu ggagacauua aagaaaguac agucuguugc aagccugcau      900

aacauccucc acggcaagga auuuauuucc gaggcuaaaa ccgaagccua cgagacucac      960

cggcgagauc uuucagaacu gaagaaauau gugcgcgaga auuguccgga gaaguacgcg     1020

uuggucuuca gaaggcaagu gaagaaaggu aagaaaaaca caaguggggc cgacgaaaau     1080

gagaaggacg auaaaaaggu gaacaauuau gcagcuuaca ucggaaugga caagggcaaa     1140

ggcuuugaaa agugcaaaaa ggaggauuuc uaugccuuuc ugaaaaagga gauagggauu     1200

aacgacgaaa gccuacucgc uaagaucgau gagggaauuu uccugcccaa acagaucucu     1260

gccgaaaauu gugacauccc uuaccagguc caucuugcag agcugaaggc cauuuuggcu     1320

aacgcggaaa auuacuuccc auuucucaaa cagaaggaug agaacugcga gaugaccguu     1380

uccgagaaaa uaaucucacu gaugaaguuc cgggugcccu auuacguagg uccuuuuacg     1440

gacaaagaaa cucgcuucag cuggaugauu aagaaggaag gcuuaaguac ccagaaaauc     1500

acaccaugga auuuugauga cguggucgau agagacgcau cugagaagaa auucauagaa     1560

aggaugacua acuuuuguac cuaucugaca ggggagaagg ugcuacccgc cuccagcuuc     1620

cucuacucgg aguaugcuuu ucugaaugaa cuuaacaacu ugaaaguuaa uggaaccaag     1680

gaucugcgug ugaagaacau ucucuucgac uuugccaaaa cgaauaagaa acugacucuu     1740

aagaaaugcu gugagcaacu ggucaagaac ggcuugcucc cugaagguuc uaaggcacgg     1800

gacguguucu ccgggacaga uggcgacuuu aaaaauucac ugaguagcua caucgauuuc     1860

aagcgaauca uuggagacaa agccgauuua uauagagaca ugugcgagaa gaucauucug     1920

uggauaaccg uaaucuccga uaaagaaagg cuaguuaaga uuaucaagga caaauacggc     1980

acugugcucu cugaugagga gaucaagggg cugaaagcuc uuaacuacac aaagugggga     2040

cgcuugagcg aaaaacugcu cgacgguauu uucgagaccg auucagaagg cgaggucggg     2100

aaugugagua uaaucgagaa gaugcggacg gacaccgaaa acuuuaugga gcuguuaucc     2160

aauaaacaca acuauaagag agccauugaa cuggcaaaua gcgagucuau cgucgaugaa     2220

agggugacau accgcgcggu ugaggagcuu uauuguucac cagcugugaa gcgaaguauu     2280

uggagcgccu ugaaaaucgu acgggaaaua gucaaggugc agggacauuc cccgaaaaag     2340

aucuucguug agacuacccg ugaaaaaccc gacgugacaa gaaagggcca aaggacuguc     2400

ucucgcaagc agcagcuccu ggacaaauac cgggagaacc uaagaaauga aaccgagcug     2460

auuaagaaaa ucgaggaaac agaggauucg aaguuuaaca gcgacaaacu cuucuuuuau     2520

uucacccaga uggguaagga ugcauacacu ggggaaaaga uugaguuuga cgauguguuc     2580

aaaacggaaa aguaugacau cgaucacaua uacccuaggu ccaaaauuaa ggacgauuca     2640

cuggacaauc gaguacuugu gcgccggaaa aucaaccaaa guguuaagaa ggauucuuac     2700

ccauugagcg ccgagauuag aaacaggaug gagggccugu ggcgucaguu acgcgacaaa     2760

aagcucaucu ccgacgaaaa auuuuaucgg cugacaagau caacuccccu uacugagaag     2820

gaaugcggag auuucauaaa ugcucagcug aucgccacca gccagucugc aaaagcugcc     2880

auucaauugc ucaagaggau gcugccugag uccgagaucg ucuacaguaa ggcgggcaac     2940

gugaaugaau uuaaaaagcg acuagaguuc gugaaaauuc gggaacugaa cgaccuccac     3000

caugccaagg augcuuauuu caauaucguc guugggaacg uguuuaauac aaaauucaac     3060

cacaauccag caguguuuca gcgcagcaac ggaaccggua auuacgacuu cgagagacug     3120

uaugauuuug acauaacuaa cgccuggaag aaaggcgaug aaaauaggau ucggucugua     3180

gcugagaaga acacaugucg agucgugaga uucuccaaga cggagaaagg gcaguuuuac     3240

gacgccacca ucuaucccgc aggaaagaau gauaaacuug cgccgguuaa gggcaaagcc     3300

ccuuugaacg acacuucaaa guacgguggg uacgauaagg aaacaaccgc uuauuucaug     3360

cuggugaaaa gucgcgacaa gaaaaagaaa gauaagacau uacucagccu ggagugcguc     3420

acugugcuug ccgacaagaa cuuuaggacc gcagaagaca aacugaagua cugcgagaaa     3480

gaacuaggcc ucaaggagcc cgagauccug auugagaaaa ucaaguugaa uacgcuguuc     3540

aagauuaacg gaucguaugc uuggauacgu ggccggacca augauagauu aguucucugu     3600

aacgccaauc agcuguggcu ugacaacggg uccgcaaaag aacugaagaa aaucucuaau     3660

cauuuuaggg augugaagaa auacaacaau aaggacuugc caguacgcga agaggaguuc     3720

agcaaagaac aaaaccucgc ccuguaugau cuauuuacag agaagcuggg aggcaagauu     3780

uacgcugguc uuccugucca ggggcagauc aaaaaucucg aaaagggacg agacaaauuc     3840

gaggcgcugg aauugaagga ucagcuguuc guguuaaugg agauucucaa auuuaugcaa     3900

ugcaacucaa ugaccaguaa uaucaagcug auaggcggug ccgagaacgc aggcaagaau     3960

agaaucucca aacuuauuca ggacucugau gugaagauga ucuucuguag ccccaccggg     4020

uauuuuugca aagucguuga cuucaagcgg uuucuguga                            4059


<210>  3035
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3035
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggaugggc cuucauaaag       60

gagcccgaaa aagauguggu cgguuccgag aucguugaca ugggcgugag aauuguaccu      120

cugucuucag augaagagaa cgacuuugcu aaggggaaua cuaucaguau uaacgcagau      180

aggacguuga aacggggagc ccgccgaaau uuacagcguu ucaagcaaag aaggaacgcg      240

cugcuagaaa ucuuuaagga gaaaaagcuc auaagcacca auuucaaaua cgcugaggac      300

ggcccauccu cuacauuuuc gacucugaac cuucgggcca aggcagccaa agaaaagauc      360

gagcugcagg auuuggugaa gguccuccug cagauuaaua aaaagcgcgg uuauaaauca      420

agcagaaagg cuaaaaguga agaggacgau ggguccgcaa ucgacucuau gggaauugcc      480

aaggagcugu acgaaaacga ucucaccccg ggccaguggg uguaugaggc uuuacaaaaa      540

ggcaggaaga auguucccga cuucuaccgg agcgaucugc aggaagaguu uaagaaaauc      600

gugaacuauc aguccgaguu cuuuccugac auauucaaug ccucauuugu cgaagacugg      660

auggggaagg cgaguacacc aaccaaacag uacuucaaca agaaaggagu gcaacuugca      720

gagaauaagg guaagcgcga ggaacgaaga uugcaggaau auaaauggag ggccgaggcu      780

guaaacuuua agauugaucu gagcgaaauc gcccuaauuc ucucucagau caauucccag      840

aucucaaaca gcaguggcua ccugggggca auuucugacc gguccaaaga gcuuuacuuc      900

aagaaccuga cuguuggaca auauuuguac cagcagauua aaaagaaucc ccacacacgu      960

cucaagggcc aaguguucua ucgccaggau uaccuggacg aguuugaaag aaucuggagc     1020

guccagucgu cuuucuaucc ucaguuaaac gaugcucuga aaagggaggu gcgggacauu     1080

accaucuuuu uccaacgacg ccucaagucc cagaaacauc uuauaucaaa uugcgaauuu     1140

gaggaucacc auaagguggu cccaaaaagu caccccguuu uccaggaauu uagaauuugg     1200

cagaaccuga auaacuugcu acugaucaag aaggacaauc ucaacgagaa auucgaucug     1260

gagcuugaaa gcaagaucgc ccuggcgaau gaguuggccu uuaaaaggga acucaacgug     1320

aaggacgcac ugaaaauuuu aggucugaag ccuaaugagu gggaguucaa cuuuacgaaa     1380

aucgaaggga aucggacuaa ccaagcauuc uuugaugccu ucgcuaagau uauagagcua     1440

gaagacggag agccaaucga ccucggcgau cugaaggccg acgauauucu ugaccaguuc     1500

uccgaagcau uuuugcgcau cggcaucgau accgagcugc uccagguaaa uucugacauu     1560

gagggggccg aauacgagaa acagagcuau auacaauucu ggcaucugcu uuacucaagu     1620

gaagaugacc agaagcugaa auugaaccuc aucagaaagu uuggauucaa accggagcac     1680

gcuaagauuc uggcguccau cagcuuacag gaugaccacg caucucuguc aaguagggcc     1740

auuaagaaaa uccuacccca ucuccagagc ggucugauau acgauaaggc uuguacauau     1800

gccggcuaca accacuccuc uucguuuacu aaagacgaaa augagaagcg ugagcuucgg     1860

gcagaauugg agcugcucaa aaagaacagc cugcgaaauc cuguggucga aaagaucuua     1920

aaccaaauga uuaauguggu uaacgccauc cugaaagauc cagagcuugg gagacccgac     1980

gaaauuaggg uggagauggc ucgcgaguug aaggccaaug cagaacagcg gaaaaacaug     2040

accuccaaua ucgcgucagc uacaagagac caugauaagu auagggagau acucaaaagu     2100

gaguucggac ugaagcgcgu caccaagaac gaccuacugc gauacaaacu cuggcuggaa     2160

acggauggca uuucucuuua uacugguaag ccuaucgagg ccagcaaauu guuuuccaag     2220

gaauacgaca uugagcacau cauaccaaaa gcacggcugu ucgaugacuc auuuagcaau     2280

aagacaaucu gcgaacguca guuaaacauu gauaaagcca augugaccgc uuucucuuuu     2340

cuccaaaaca agcuguccgc cgacgaguuc gaacaguauc agaguagagu aaagagccuu     2400

uacgggaaac ugucuaaggc aaaaauccag aaguugcuca uggcuaauga uaaaauuccc     2460

gaggacuuua ucgccaggca acugcaggag acucgcuaca uauccaagaa ggcgaaagaa     2520

auucuauucg agaucucacg gagaguuagu gugacaaccg gcacgaucac cgauaagcug     2580

agggaagacu ggggacucgu cgagauuaug aaagaacuga acugggagaa guaugauaaa     2640

cuuggcuuga cauacacuau cgaggggaag cacggagaac gacugaacaa gauuaaagac     2700

uggagcaagc gcaaugacca ucggcaccau gccauggaug cuuuaaccgu ggcacucaca     2760

aaaccggccu auauacagua ccugaacaau cuuaacgcua agggucugaa uaacaaaaag     2820

ggcacugagg ucuucgccau cgaacagaag uaucuaaaaa gagagaaugg gaagcucugu     2880

uuuauuccuc ccaucgaaaa cauuaggucg gaggcuaaaa agcaccuguc ccguaucuug     2940

gugucuuaca aagcgaagaa uaagguugug accauaaaca aaaauaagac aaaaagcaag     3000

gccggacuga acgagcaaau cgcuuuaacc ccacgcggcc agcuccauaa agaaacugua     3060

uaugguaagu cauuccacua caguacgaaa uuugagaaga uuggggccuc cuucaauguc     3120

cagaagauca acacaguggc uaaaaaggag gaacgggagg cacugcuuaa aagacuggcc     3180

gaaaauggca acgacccuaa gaaagcauuu accggaaaga acacuuugaa uaagaugccc     3240

auuuaccucg aucugggcaa aaacaucaag cuaucugaga aaguuaagac cgugguccug     3300

gaacagaauu auacaauaag gaaaaacauu gacccagauc uuaaggugga caagguaauc     3360

gaugugggga ucaaacgaau acucgagagc cggcuggaag aguucggagg uaaugccaag     3420

uuggcuuuuu ccaaccugga ggaaaauccc auaugguuaa acaaagagaa gggcaucuca     3480

auuaaacgcg uuaagaucag cggggucagu aauguggaau cucuccacgu gaagaaagac     3540

cauuucggag agccuauucu ggaucaagaa ggcaacgaga ucccagucga cuuuguuucc     3600

accgguaaua accaccaugu ggcgauauac gaggaugaaa augggaaccu ucaggaggaa     3660

guggucagcu ucuuugaggc cguagugaga cagaaucaag gccugccgau cauuaagaaa     3720

aaccacacuu ugggauggaa guuccucuuc acacugaaac agaaugaaua uuuuguuuuc     3780

cccucggacg acuuugugcc ugcagauguc gaccuaaugg augagcagaa cuaccaccug     3840

aucucaccaa accuuuucag ggugcagaag auugcucgga aaaauuaugu uuuuaacaau     3900

caucucgaga cgaaggccgu ggacaacgau cuguugaagu cuaaaaagga auuaaguaaa     3960

aucaccuacc acuucuauca aacucccgag caucugcgag gcauaauuaa gaucagaauc     4020

aaucaccucg ggaaaauuau ccagauagga gaauacuga                            4059


<210>  3036
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3036
augaacaaga aauacuaucu gggccucgac aucgggacca auagcguggg augggccguc       60

acagaugaga acuacaaucu uuauaaguac gcugguaaaa gaaugugggg cauuaggcug      120

uucgaagcag ccgacacugc ggcugagcgg cgcaugaagc gauccaaccg uagaagguug      180

ucucggaaaa agcagcgcau agauuuacug caagaacuau uuucagagga aaucguuaag      240

aaagaccuca cguucuuuau uagacugaau gagaguaggc uucacuucga ggauaagagc      300

accggggaaa aauauccccu guuuguggac aaggaguaca cagauaucga cuaucauaaa      360

caguacccua cuauguacca cuugcggaag gaacucauug agaacccaga gccgcaugau      420

auccgccugg uauaucuggc cugccaccau auacucaaga aucgaggaca cuucuuaauc      480

aacggcucca uuucugacgu gaaaaaucug ucguuugucu guggugaaau gaucaaggag      540

uucaacucag ugagcgaaua cgagauuagu cuuaccgaua ucagagaauu ggagaggaua      600

cuguccgaca aaaagacagc aaaaucuguu aaggccaaac agguggcuca gcuauuugag      660

ugcacccccg cagaagauaa ggagcaacag aagugugcca aagcuauuau cacuaaucuc      720

agcaagcuga uugucgggcu uaaaggaaac aucuccaaga uguuccacca gccucuggaa      780

gagaucucaa caaaaaguuu uaaguucacc gaagccaagu augaggacga gauuaugcca      840

gcgauagaag agaauuaccc cgacuaugca agcgugaucg aucggauuaa aggcguauac      900

gacuggucug uuuuggugga aauccucgau ggcgaggacu aucuguccuu ugccaagguc      960

aaggcuuacg aaaaacaugg gaaaaacuua cacauacuga agaaugugau gaagaaauac     1020

ugcaaggaug agguguauca ggccuucuuc aacggaauua acgagaaaaa ugguuacggc     1080

aacuauaucg gggcaauccg uaagaaugga cauacguaca aaauggaccg cuguaaguca     1140

gaagaggauu uuuauaaggg ccuccagaaa auucuuagca cuaucguccc ugaccaagaa     1200

gaugaggagc uguuggaaaa gcuaaaagcu gagguugaaa accagacccu gcucccacug     1260

gccagaagua aggagaaugg uacaauuccc aaccaggugc accuucaaga acugaugaaa     1320

auauuggaca augcgaagga guaccucgau uuccugaaaa aggaggacga auaugggacu     1380

aucgccgaga agauuaucuc uaucgcaaaa uuuaggauuc cuuacuacgu aggaccauua     1440

ucccggcgac acaaggaaga gggcagcaac ucguggauga uacgcaaaga agagggcaga     1500

aucuauccgu ggaauuucgg ggacaaggug gaugucgagc agucuaacaa agaauuuauu     1560

gagaggauga ccaauaagug cacauaccug aucggagaag acgugcuacc caagaacucc     1620

cuccuguauu caaaauacau gguucuuaau gaguugaaca aucugaagau ucgggguaac     1680

aaaaucagug uggaacucaa gcagcgcaua uauaaagauc uguucuguac cauggcuaag     1740

gucacgggca agagaauccu ugaguaccug aaaauggagg acccugaaau ugugcaagag     1800

gauaucagcg gguuugacau ugauuucaag uccgccuugg cuucuuaucu cgacuuuaaa     1860

aagcagguag uuggaaacga aaucgagaaa gaucauuaca aggagauaau ugaagagauc     1920

auuaagugga aaacuaucua cgacgaugac agcaagauga ugaggcguau gauagaacgg     1980

gaguauccag aaguguucga uaaagagcag aucaagaaaa uuugccagcu gaaguacuca     2040

ggcuggggua auuuuaguga ccgauucuua aacgggguca gaggcgccga caaagagaca     2100

gaagaguccu auaccaucau ugaagcacug uggaagacua auuacaaccu aaugcaacuc     2160

cugagcaagc aguuuucuuu caaagaggaa aucgaucaga uaaaugccga gaaggcugga     2220

gacgugggca aaauuaacua ugagaauaca gucaaggauc uuaucguguc acccaguaac     2280

aaaagggcga ucuggcagac cauucaaauc gcagaagaga uuaagaaggu uaugaaaugu     2340

gaaccugagc gcauauucau cgaaauggcc agagggggag agaaggagaa aaagcggacg     2400

gugagcagga aaacccgcuu gcaggaacug uacgcugguu gcaaggacga uacacgaaau     2460

uggaugaagg agauuggcga ccgggaagaa cgugaguuua acuccagaaa acucuaucug     2520

uacuacacuc agauggggaa guguauguau accggagaug aaaucgacgu agaugccuua     2580

augaaaggca auucuaacug ggacauugau cacaucuacc cacagucgag gauaaaggac     2640

gauagccugg acaauauggu ccuugugcgc aaagacguua acaauaagaa gcggaacaaa     2700

aucuuguccc ccaagauuag agaggauaug aaagguuauu ggacaaugcu cugccaaaag     2760

gggcugaucu caaaaaggaa guacgaccga cuaacucgca ccacagauuu caccgacgag     2820

gagcugagug gcuuuauuga acggcagcuc guggagacua gacagucuag caaggcaguc     2880

gccgagcugc uuaacagguu guauaaagau uccgaagugg uauacgugaa ggcuggacug     2940

guuucagacu uccgucauga uaaugacuua cucaaaagcc gccgggucaa cgauuaucac     3000

caugccaagg acgcauuucu gaauaucgug gugggcaacg ucuaccacgu uagauucacg     3060

ucuaauccga ggaaauggau aaaggauaac cgagacucca auuacaguau ucggcgcgug     3120

uuuaacuaug acgugaaaca gggggauaag aucguauggc acggaagcga gaaugguggc     3180

cauucuaucg accaaauuaa gaaaauaaug uccagaaacg auauccuuua cacagaauau     3240

accuacugcg agaaggggca gcuguucaau gaaacuuugg cgaaaaaggg aaacgagaaa     3300

gcuauugccc ucaagaagaa ucuggacccu gucaaauaug gcgguuacac cacacccaag     3360

accucagcau uugccuucgu ggaguuugau gggaaaaaga aagaaaggaa gaaccacauc     3420

guugagauuc caguguaugu cgugaauauc gcuaagcggg aaccugacgc cguuaaaaag     3480

uaccuagagg aacgaaaagg cuacaguaac gugguaauaa agaaauaucc caucaagaag     3540

aacagccuga uuaaaaugga uggauaccuc gcaagacugc gcggcgcuaa ugagaaggac     3600

aucauucuua aaaacgccgu ccaguugauc cugaauaagg auuuauauga gauagugagg     3660

aaaauugaaa aguaccucga guauaaccug caauaccagg cggacgccaa auucgaaggg     3720

uuuacuauga aggaucuuaa uaugaucuau gaggagcugu accuaaagcu caaagaaucg     3780

aucuuccaga agcguccagc ucagcaaaug aaaacacugg agaagggaaa agaagcauuc     3840

auuuccaucg agaacuuggg cgaaaaggcc aaggugauaa augagauuau cgcuauguuu     3900

cggugugacg ccacgaccgu cgcaaaccug aaaguuauua augguucuca gaacgcgggg     3960

agcaugaagg ugaauaaaaa cacuuuaaca ucaaagaguc ucaaacuggu acaucagucc     4020

aucaccggac uuuucgagac agaagaggaa cugguguga                            4059


<210>  3037
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3037
augaagaaaa aguacuaucu gggccucgac aucgggacca acagcguggg augggccguc       60

acagaugaga auuacaaccu uugcaaauuc aaugguaaga aaaugugggg cauuagacug      120

uuugaagacg cuaagacugc agccgagagg cggacgaagc gcuccaaccg acguagaaua      180

gcgaggacca aacagcggau cgauuuguua caagaacugu gugagaaguc uauuuucgaa      240

aaagacccca cauuuuucau ccgccuaaau gagucaaguc uccaccugga ggauaagagc      300

acuggggaaa aauauccucu uuuuguugac aaggaguaca ccgauauuga cuaucauaga      360

gaauacccaa caguguauca ccugaggaag gaguugaucg agaacccgga accccaugau      420

ccucggcucg uauaccuggc uugcugucac auaaucaaau cccgcggaca uuuccuggug      480

aauggcucua uuucggacgc caaggaucuc ucauuuaucu gcgagaacau gauuaauagc      540

uucaacgcag ccagugacua caaaaucaau auacaggaua ugaccgcuuu agaaacuauu      600

cugacagacc gaaagauggc aaaauccguc aaggccaaac agaucgugca gcuuuuuaag      660

uguauuccau gcgaggacaa ggaacaacag aaauguugca aggcuaucau caccaacuug      720

ucuaaacuga uuguuggucu aaagggggau auaagcaaaa ucuucaauga guccuuugag      780

gaaauuaagu cauucaaguu ugccgagacg aaauaugaag acgagauccu ccccacuaug      840

gaagagauau acccugauca cgcggagauu aucgaaaaga ucaaaaguau uuaugacugg      900

agcauccugg augagauucu ugacggagaa gaguaccugu cuuucgcaaa ggugaaagcc      960

uaugaaaagc acaagaaaaa cuugcaugau cucaagauac ugaucaaaaa guacugugac     1020

gaugagaccu aucaguccuu cuuuaauggc augaacgaga aaaacaagua cucaaauuac     1080

auuggcagca ucagaaagaa cgggcacaca uauaaagucg aaaagugcaa aaguucugag     1140

gacuucuaca aggaauuacu gaaacuccuu uccagcguga auccaggaca ggaggaugag     1200

ucgcuguugg aacaaaucaa guuugagacu gaaaaccaga cccuacugcc ccuccugagg     1260

acaaaagaga augguaccau uccuaaccag guacauaugc aggaacuuau aaagauccug     1320

gacaaugcuu cuaaguauuu ggacuuccuc aacgagaaag augaguacgg cacgacugcc     1380

gacaagauua ucuccauugc aaaauuuaag aucccauauu acguugggcc gcugucagau     1440

cggcacaaag aaaauggaag uaacagcugg auggugcgua aggagaaugg ccgcauauau     1500

cccuggaacu ucgacgauaa ggucgacgcu gaaaaaucca auaaggaguu uauccaaaga     1560

augacaaaca aauguaccua cuuaauuggu gaagaugugc ugccuaaaaa uucucuacuc     1620

uacagcaggu auauggugcu gaacgagcuu aacaauuuga agauccgggg gaacaagauu     1680

ucagucgagc ugaagcagca gaucuacaau gaccucuucu gcacuaaagc ccgaguuaca     1740

ggaaagaaca uacugaauua ucuuaaaaag gaagauccag agcuggcggc cgaagacuug     1800

aguggcuuug auguggacuu caaauccgca cucaccagcu accuggauuu uaagaagcaa     1860

guagugggca acgagauuga aaaagacuau uacaaggaga ucauugagga aaucauaaaa     1920

uggaagacga ucuauaauga ggacucuaaa augaugaagc gcaugauugg gagagaauac     1980

cccgagaccu uaaaccagga ucagaucaaa aagauuugcc accugaagua caauggaugg     2040

gguaacuucu cagagacauu ucuaaauggc aucaaagggg cuaacaagga aacuggagag     2100

uguuauacca uaauugaagc ccucuggaaa acaaauuaca accugaugca gcuuuugagu     2160

aagcaauuca gcuucaaaga ggaaaucgac cagaucaaug cugagaagac uggcgccauu     2220

gguaagauca ccuaugagaa ccuggucaaa gaucucgugg uuuccccugc aaacaagagg     2280

gccauuuggc agacaauaca gaucaccgaa gagauuaaaa aggugaugaa augcgaacca     2340

gagcggaucu uuauugaaau ggcucgcggg ggcgagaagg agaagaaaag aacugucucu     2400

aggaagacgc gucugcaaga auuauacaca ggauguaaag acgauacccg ggacuggaug     2460

aaggagaucg gcgaucgaga agagagagag uucucgagca ggaaacugua ucuuuacuau     2520

acucagaugg ggaagugcau guacaccgga gaagagauag acguggauuu gcucaugaca     2580

gcaaauucca agugggacau cgaucauauu uacccccagu cacgcaucaa agacgauagu     2640

uuugacaaca ugguacuggu ucggaaggac auuaauaaca augugaaagg uaacaauauc     2700

gucccgucua gcauacagaa ggaaaugacc uccuauugga aaaugcuacu gcaaaaggag     2760

cucauuucaa agaaaaagua cgaaagacug acuaggagca cagauuucac ggacgaggag     2820

cuuucuggcu uuaucgaacg ccaguuggug gagacccgac aguccaguaa agcggucgcc     2880

gcucuguuaa accggcucua uaagaauagc gaaaucgugu acguuaaagc cgggcuggug     2940

ucugauuucc gucacgacaa caaacuucug aaguccagaa ggauuaauga uuaucaucac     3000

gcaaaggacg ccuuuuugaa cauaguaguc ggaaauuguu accgcacuaa guucacagcu     3060

aacccucggc aguggaucaa aaagaauaga gauaccaacu auucaauuag gcgaguguuu     3120

gacuucgaua ucaaacaagg cgacaagauu guuugguacg guaacaaaaa ugagaacggg     3180

aagcauacaa ucgaucagau aaagaaagcc augagucgca augacauccu cuacacugaa     3240

uauaccuacu gcgagaaggg caaacuguuu gacgagacgc uaaccaagaa aggaaacaag     3300

aaggcaauuc cccugaaaaa gaaucucgau ccacugaaau auggcgggua cacaagcgug     3360

aagacuucgg cguucgcuuu uaucgaguuc gacgauaaaa agaaggaacg gaaaaaccac     3420

auugucgaaa ucccuauaua uguggccaau auugcaaaga gagagcccga agccguaauc     3480

aaauaccuug aggagcagaa gggauaugug aacaucguug ucaaaaagua cccaauuaaa     3540

aagaacuccu ugcugaagau cgacgguuac uuagcuaggc uccguggcgg ggauucuagc     3600

auaauuaccc ugaaaaaugc cgugcaacuu gugcuggaca agaaaucaua ugaaaucauu     3660

cgcaaggucg agaaauaccu agaucacaau aguaaguaug aagcaaacuc caagcucgag     3720

aaauucaaug aaaacgaccu gaauaucaua uacaacgagu ugugucugaa guuaagaaau     3780

ucuauuuauc agaaacggcc cguucaucag cucgagacac uggaacaggg aaucgauaac     3840

uuuaggagcc uugagucccu gaaggacaaa gcuaagauca uuaacgaauu gcucgccaug     3900

uuccgaugcg augcgaagac uggcauggac cugcggcuaa ucgguggggc cucaaauagc     3960

ggccgcauga gaauuaguaa aucuaggcug accuccaaga gccuuaaacu cauaaaccaa     4020

ucggugacgg gacuguuuga gacagaggaa gaguuggugu ga                        4062


<210>  3038
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3038
auggagaaga acuacuaucu gggccucgac auggggacca gcuccguggg augggccguc       60

acagaugaaa auuacaaccu uaugagaaag aaggguaaag accugugggg cguuagguug      120

uucaaggagg cuaaaacuuc ugcagaacgg cgcgugaagc gaacgucacg uagaagguua      180

cagcgggaga aggcccgcau cggguaucug aaagaacuau uugcggauga gauuaauaag      240

guagacgcug gauucuacca aagaauggag gauaguaaau uuauaagcga cgauaagacc      300

gugcaccaga aauucacacu cuuuuccgac acuaacuaua ccgaugaaga cuacuauaau      360

gauuacccca caaucuucca ucugaggaag aaacuuauug agucugacga ugaauacgac      420

guccggaugg uguuucuggc cuugcucaac auguauaaac accgcggcca uuuccugaau      480

gcaaaccugu cggacaaugg uaugggggau aucaacgagc aguacgaccg acucaaggag      540

uuaugcucag aauaucugaa uguugagugg gaaaacaaac cugauaaguu ugaggaaauu      600

cuuaaagagu guaauugcag caacaguuug agaaaugaga ggcugcuaga aaccuucaac      660

cucaagaaaa cugacaagga gaagugugaa augcugaaac uuaucugcgg acuguccggc      720

acauugucua agauauuuaa agaucucgac gaugacagcg agaccaagaa aaugacgauc      780

uccuuccggu caaguaacua cgaugaagag gccauuaaug uggaggaaau cauuggcagc      840

gagaacuuug aaaucauagc uguccugaag gagauucacg acuggucuau guuagcaaau      900

aucaugcgug ggaagaaaua ucuguccgug gcccgcguag aagaguacga gaagcauaaa      960

gaugaccuca agauucuuaa aaagguuauc aaggaauauu caaucgagga auacaacaau     1020

uucuucagag ugauggcuga caacaauuac agcgccuaug ucaacagugu gaauagcaac     1080

aaagagucca cuaggcgggg agcgaagugu gauaaugaca ccuuuuucaa agagauuaag     1140

aaaauaguga acaagugcua cgauagcgaa gagaaggaau auauccugaa agagauugaa     1200

aaggguacau uuuugccaaa acagaucaua ucgucuaaug gcgucauucc gaaccaaguu     1260

cacaagaucg agaugaaaaa gauccuacag aaugcagaga aauaccugcc cuuccucacu     1320

gaaaaggacg auuccgggcu gucagugagu gagcgcauuc uucagauguu uagcuuccag     1380

aucccuuauu acauuggacc aauauccggc aagaucgguu cugacgaaaa aucuaagaac     1440

ucauggagug uacgaaaaga gaaggggaga auuuaucccu ggaacuuuaa agauaagauc     1500

gacgaaaagg aguccgccga gaaauucauc gaaaggaugg ugaaucacug uaccuaccug     1560

aacaaugaga uggucuugcc uaagaacagc cuccuguacg aaaaauuuau gguguuaaau     1620

gagcugaaca aucuaaagau uaacaaugaa aacauaucug uugagcucaa acaaucacug     1680

uauaauaacc uuuucaagac aggaaagaaa gugaccgcua agggcaucaa aaguuacuug     1740

gagcggaaug gccugugcga accaaacgag gauauuaauc ucacggggau cgacggagau     1800

uuuacuaacc gccugagcaa cuaugccaag uucaaaggua uuuuuaauac agacgaacuu     1860

accuacgagc aguggaacau ggcagagaag aucauaaagu gguccacuau cuauggcgau     1920

ucuaaaaagu uccuggaaga gaaaauuaga aaugaauacu cggagaaguu ggacagccag     1980

cagaucaaaa ggauucucgg gaucaaguuc cgugacuggg gacggcuguc cgaagaguua     2040

cugaacaugg agggcgcuga ugucaauaca ggugaaauaa aaucagugau ugggcgaaug     2100

ugggacgaga acuauaaucu aauggaacuc aucgccaccg auaacuuuac guacaagcaa     2160

guaauuaagg agaaacagaa uggccuggac aagagucuuu cugaaaucga uuacgacgau     2220

uuggacgagc uguauguuag cgcgcccgug aaaagaauga uauggcagac ccuccugauc     2280

auuaaggagu uagucuccgu gaucggauac gaaccuacaa gggucuucgu ggagauggcc     2340

aaagauguua acacugaaaa gaagcgcacc gacucacgga aaaagaaauu ucuggaucuu     2400

uauaagcaau gugagaaaga cgaaagagau uucuggauua aggagaucga cgacaagagc     2460

gaggcagaau uuaggaaaaa gaaauuguac cucuauuaca cacagaaggg ccgcugcaug     2520

uauacugggg agcagauaaa uccacuggau cuaaccaacg acaaucugua cgauauugac     2580

cauaucuacc cgcgacacuu cgugaaagau gacucuaucg aaaacaaucu cguacugguc     2640

aagaaggaga aaaacgcuca uaaguccgau acauuuccca uugaggacga uauccggaaa     2700

aaccaguaug ccuucuggaa gaugcuuuac gagaaaaauu uuauuaccaa ggugaaguau     2760

caacguuuga cuagaaaaga gccuuucagu gacgaagaga aggcuaacuu uauaaugagg     2820

cagaucguug aaacgcgcca gggaacaaaa gugauuaccg auauccugga aaagagcuuc     2880

gacaaaacuc agauugucua cgugaaggcc gguaauguau cuuuauuucg gcaaaaauau     2940

gaguguauca agugcagaga aauaaacgac uuccaccacg caaaugaugc cuaccucaac     3000

aucgugguug gcaaugucua ugacaccaag uucacauccg auccaaggaa cuuuauuaaa     3060

gaguaccgaa agaaucccca gaaaaacgaa uaccauaugu auaagcuguu cgacuacaau     3120

gugcagcgcg gggaucgggu ggcuuggacc gcgucaaacg gagagaguau gaaagucguu     3180

aagggcacuc uuaagaaaaa uacaccucug gugacguuua ugagcuauga ggaacacggu     3240

ggguuguacg accagaacau cauuucggca aaggauaucg agaaagugaa gggcaaagga     3300

uaucucccaa uaaaguccuc ugacagcaga cugaauaaca ccgaaaagua cggcggguau     3360

gccaaaguau caggagcuua cuucuuuguc guggagcaua cuguuaagaa ugugaggauu     3420

cguacaaucg aacccaugcc gugcuaccua accaaagucc ugacaacuaa ggagaaaauc     3480

gaagauuauu guugcaacau ucucaaguac gacaguccug ugguuaagua ugagaaaaua     3540

aagauguacu cccugaucaa agugaacggu uaucccaugu accuuaccgg ccgcacgggg     3600

aaucaauuga uuguaucuaa cggaauccag cuggucuuaa gcuaugauga ggccaaguac     3660

auuaaagacc uccugaagau caaucaggaa gaguucgauc agaaccggcu ugaaaaucug     3720

ggcauaaaca aauuuagaaa ugagaagcua uacgacaccc ucaacaagaa auauacaaau     3780

gagaucuaca agaaaaggcc aaacuccaug gguaauaaga ugauugaaug gaaaaacacu     3840

uuccaaaauc ugucaaucaa ggaccagacc gagguguugc ugcaguuaau ucaacucggg     3900

cagaagcaga acagcggcgu gaaucuggaa aaacuuggag caaguaagaa aacaggcguc     3960

ucucugguuu ccaagaaaau cacugauaag gagagcuuug aauugauaaa caagucgauu     4020

accgggcucu augagaacaa aguggaccug cuacgaguau ga                        4062


<210>  3039
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3039
auggccgcuu ucaaguacua ucugggccuc gacaugggga ccaacagcgu gggaugggca       60

gucacagauc ccaauuacaa ccuucugaaa gccaagggua aagacuugug gggcaucaga      120

gaguuuaaug aagcguccac ugcuguugag aggcggacgc accgcauuuc ucgacguaga      180

aggcagcggg aacaagugcg cauaggguua cugaaggauu auuuccauga cgccaucgga      240

gagguagauc cugacuuuuu ccagagacua gcaaacucaa aauaccaccu cgaagauaag      300

gacaccgagg ugagguauaa gaauaacguc uuuaaugaug acgauuacac agacaaagau      360

uauuucgacc aguacccaac uauuuaccau cugcggaagg agcuuaucga aaguaccgac      420

aaacacgaug ugcgccuggu guuuuuggcc cuccugaaca uguucaagca ucgaggccac      480

uuucugaaua gcggucucgg ggagaacucc ggagaaaaua acauuaauaa cgcuuauuua      540

gagcugacaa aucuuuuguc ugaguucacc caguacaacc ugaacgaaac uaucgacggc      600

aaaaagauag aggauauccu aucgagaagg gacaugucac ggacacguaa ggcagaaggc      660

cucgccgaga uucugggggu ugauuauaaa aauaagccgu acaaagaacu uauccgcgga      720

cugugcgguu ugaaguuuaa cgcuugugcc auuuuccccg agauccaaag cgacgagguc      780

ccuaaacucg auauaugccu gagugaagcg uccuuugacg agaagucuga ugaaauugca      840

aauaucuuag gcgaggacua uuucgaaauu aucauggcca ugaaagauau cuacgacauu      900

gggagccugg cuggaauaau gaagggccac aacuaucucu cccaggccag agugaccuca      960

uacgagaagc auaaagagga ccuuaggcug uugaagaaug uaauccggaa auacuguagu     1020

aaggaagauu augacaacuu cuuuaauagc gaggcagaug guucuuacgc uuccuauguu     1080

gggucauaca acagcaaaac gaagcagcga cgcgugggaa auaagagaag uucugaggag     1140

cuauauaaag ccauuaagaa aauccugaag aacgucccag cggaagacgc cgaugugcag     1200

aggauacuca uguccauuga gaaugagacu uuccugccca aacaacuuac cgcaagcaac     1260

ggcgugaucc cuaaucaggu ccacgcuaag gaaaucaaga aaauucuguc gaacgccgag     1320

cgguacuugc cauuucuccg cgaaaaggac gagacaggcc ugucuguuuc cgagaaaauc     1380

augaaguuau ucucauuuca gauucccuau uacguggggc cuacuaccga aaaaucaagc     1440

aaggauggag guaauggcug gguagugaga aaggaggaag ggcagguccu gccauggaac     1500

auagacgaga aaaucgauau gaaggcuaca uccgaagccu ucauuucuag gauggugcgu     1560

cggugcaccu acaucagcgg agagccgguu cuacccaaau caagucucga guaucaaucc     1620

uuuugugugc ugaacgaaau caauaacauu aagauagacg gcgagcgaau cagcgucgaa     1680

cuuaaacagg auauuuacaa ggagguguuc cagaaaggua agagaguaac gaagaaacaa     1740

uugugcaagu aucugcauac uagggggauc auugaagcag aggagcaggu uacaggcauc     1800

gacgugacca uaaauaacgc ccucacuaca uacggaaaau uuaaggcuau ccugggcgau     1860

gacauuucua aagacucagu ccagaagaug guggaaaaua ucgucuucug guguaccgug     1920

uauggggaua guaagaaauu ucuuaaggac cgcauugagg aaaaauacgg agagaagcug     1980

acgaaagaac agaucaagcg gauaguuggu uucaaguuua aagauugggg caacuugagc     2040

aagaauuucc ucgagcuguc cggggcggac gugucuaccg gagagucggu aagcauuauc     2100

agagcauuau ggaacaauaa ccugaaucua auggaacuca uuaacuccag gcuguauaau     2160

uacaaagagc gccuugccga auaccaaaac acaaugauga agacuuuguc agauaucgag     2220

gcugaggacc uggaugaaua uuacuucagu gccccuguca aacgaaugau auggcagacc     2280

auccucauua ucaaggagcu ggugaagguu uuaggcugcg aaccagaccg gauuuuugug     2340

gagaugacac guagacccga ugaaaggaaa augcgcacug agucucggag aaagaaauuc     2400

gaggaacugu auaagaaagu caaggacgag gauguggacu ggaugaaagu aaucgcacac     2460

gccgaugaaa ccgguagcau aagguccaag aagauguacc uuuauuugac acagaaaggg     2520

cgauguaugu acaccggcaa gcacauugag cucucagacc uguuuaauaa caaccuauau     2580

gacaucgauc auguguaccc ucgccacuuc guuaaagacg auaauaucga caacaaucug     2640

guccucgugu gcaaggagaa aaacgcucau aagagcgaua uuuacccacu ggaagacgag     2700

auccuugccu cucagaagaa uauguggucc gaauugcgga gaaguggauu uauuacugag     2760

gaaaaauaua acaggcugau aggccguaau agcuucacgg augagcaaaa ggcaggguuu     2820

aucgcgcgcc aguuagugga aacaucucag ggaaccaaag gugucgcuaa cauucuccag     2880

caacugcuuc ccgaguccaa gaucguuuac gccaaagcau caaaugugag ugaguuccgg     2940

aacacuagag acauuccuaa gagcaggcug aucaaugaau uucaccaugc ccacgaugcu     3000

uauuugaaca uagugguagg caaugucuac uaugugaagu ucacccagaa cccgcucaau     3060

uuuaucaaaa acgacuacga ucgagacaag accaaaaaca auuauaaccu gucgaagaug     3120

uucgacuggg auguugagcg gaauggggaa guggccugga uugcacagaa aaaggacgga     3180

gaggcuggca caaucgccac ugucaagaaa gugcuagguc gcaacacacc ccugaugacg     3240

agauacuccu uugaggggaa gggcggacuc accaaugaaa cucuguacuc ugcgaaaaag     3300

gccaaagagg aaggcuauau uccauucaag agcucagaug agaagaugaa agacguuaca     3360

aaguacgggg gauucaccag ugugacaggc gcuuacuuuu uccuuguaga acacacugag     3420

aaaaagaaaa ggucccggau gaucgagucu gucccuuugu aucuggcaca gaagauagaa     3480

caaaaccccc augaguuaga aaaauauugu gaacaggugc ucggucugau ugauuuugac     3540

auccgaguga gaaagaucaa gauugggagc cuuauaaaac gcaagggaua cuucuuuuau     3600

aucaccggca aaacggguca caggcugauu cuacguaaug ccgucaacau gugccucaag     3660

aaagaauggg uuaaguacau cuccaagcug gagaaagcug uggauaagaa uaacguagag     3720

ggcauuauca ccaaagaaaa gaaugaguug cuguacucag aauuaauaga gaaauucaag     3780

aacagcaucu uuaguaaucg gccaaacccc gugggggaca agcucgauga agccaaagag     3840

aaguucauua aucuggacau cgagaaacag ugcauugucc uuugucaaau ccugcaguug     3900

ucuuccgugc ucggaagcga augcgauaug acacugcuag gcgagucggc acauuguggg     3960

aagauacuga uuaguaaaaa gaucggagac gaagauaucu aucuuauuaa ccagucuguu     4020

acuggccucu acgagaagaa aaucaaucug agaaccgugu ga                        4062


<210>  3040
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3040
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cuucauaaau       60

gaggcugaaa acgagaucga aguguccucu auugucaaua cagguguuag aaucgugccc      120

cugacuacgg augaggaauc agacuuuaag aaaggcaaca ccauuaguau caaugcagau      180

aggacauuga agcgcggggc ccggcgagga uuacagcguu ucaaacaaag aagggaggcg      240

cugcuagaga cuuuuaaccg gauaaaguuc aucgcuaccg acuuuguaua cgccgaaaca      300

ggcgcagcca ccacuuucag cuccuaucag cuccgcgcua agucugcagu ggaggccguc      360

acaaaagaag aacuggugaa gguucuucug auguugaaua aaaagagagg uuacaaaucg      420

ucaaggaagg cuaagaccgc cgaggaaggg gaugcgauug acggaaugaa aaucgcaaag      480

gagauuuuug aaaacaaucu cacgccuggc cagugggugu auaacagccu gaguaaaggc      540

gggaaguuca ucccagauuu uuaccacucc gaccugcaga aagagcucga aaagauaguc      600

cgguuccaaa aucaguuuca uucugagcag auuaacgaua aauuacuggc cgacauccag      660

ggaaagagcc gcacucaaac cucccagcac uucucaaaga cacuuaauau uccguuggcu      720

gagaacaaag guacucgaga agagaccaag cugcagcauu aucaguggag aaaugaagcc      780

cuagcaaacg agcucgauau gaaaauccug gcuuuuauca uuacagaaau aaacaaucaa      840

aucaaccaga guagcggcua ccuuggggcc auuucugaca gguccaagga gcuguauuuc      900

aaucaggaga ccgugggaca auaccaguac aaacaguugc aggaaaacgu acacgcgaag      960

cucaagaauc aaguuuucua ucggcaggau uaccuggacg aguuugagaa aaucugggag     1020

acgcaggcca aguuccauuc agaauuaaac gagaaacuga aggcagagau acgugacauu     1080

acuaucuuuu aucagcgcaa acucaagagc caaaagcacc uugugaguuu cugcgaauuu     1140

gagaaaggcc acaaggcuau ccccaaaucu uccccucugu uccaggaauu uagaauuugg     1200

cagaauuuga acaaugucgu ggugaagaac gagacaacca aagaggucuu cgaacuaagc     1260

gaggaacuga agcagcucau cgccgaggaa cugcaauuua aggagucgau gacugauguu     1320

cagaugcuua cauucuguga gcugaaaaag gguacccaua cggugaauuu uaaaaagauu     1380

gaagggaaca ggaccaauac aucuauauac aaagcauucg agaagaucuu ggaaguagag     1440

ggauaugaaa uugacuuuuc caaaaugaag ccacucgaga ucaaggagac uguggccccc     1500

aucuucgaaa cccugaacau uaauacaguc auauuagauu ucgacccuac uauccagggc     1560

aacgauuuug acaaacagcc auacuaucaa cuguggcacc uacucuacuc agcagaggau     1620

gacgauaagc ugaaagaaac ccuuaagaca aaauucggcu uuaaggagaa ccauuugccc     1680

uucauucuga auaucaaccu ccaggccgac uacgggaguc ugagcgcuaa ggcgauuaaa     1740

aagauccuuc cucaccugau ggauggacac auauaugaca aagcaugcuu gauggugggu     1800

uacaaucauu ccucuagccu caccacugcc cagaacaaug accggguucu gaaggauacg     1860

uuagaacugc ucaaaaagaa cucacuacga aauccagugg ucgagaagau ccugaaccaa     1920

augauuaaug ugaucaacgc uauuaugaaa gacccggcca ugggcaagcc cgaugagauc     1980

cgcguagaac uugcaagaga guugaaagcc aauaacgaac agaggaagaa agcuacaagu     2040

gacauaaaua ccgccacuac cgagcacgag cggauucgca agcugcucca uuccgaauuu     2100

aacaucccua gaguuacaag gaaugauauu auccguuaua agcugugguu agagaccggg     2160

ggaauaagcc uguacacugg caacccaauc aaagcaucug accuuuucuc aaagaacuau     2220

gauauugaac acaucauucc caaaagucgg uuguuugacg auagcuucuc caauaagaca     2280

cucugugagc gacagcugaa caucgacaaa ucuaauaaga cggcguacuc guuucuacag     2340

gaaaaacuga gcgagaacga uuucgcugcc uaugagaaua gagugaagga ccucuuuaag     2400

accgguaaaa uaaaguccua cacuaaauac aagaaacugc uuauggcaga uaacgaaauu     2460

ccugaggggu ucaucgacag gcaauugcgc gaaacacagu auaucgccaa gaaggcuaaa     2520

gagauucugu uagaagucuc acggaaugug accgccacaa ucggcagugu cacugacaag     2580

cucagacagg auugggagcu gguggacguu augaaagagc uuaacuggga aaaguacgau     2640

aaacugggau ugacccauau ugaggaaggc aagaaugggg agaggcucuc uaagauaaaa     2700

gacuggacga agcgcaacga ucaccgacac caugcaaugg acgcuaucac cguggccuuu     2760

acaaaaccag cguauauuca guaccugaau aaccuaaaug ccaagacuca aggagaaaac     2820

aaagcuaaua gcaucauugg uaucgagaac aagcaccugu aucgggauaa gaacaauaaa     2880

cuccguuuca uaucccccau gaagaacuuc agagaggaag cuaaaaagca gcuggaguca     2940

auccuuauua gcuacaaagc caagaauaag guagucacca aaaacaagaa uacaacuaaa     3000

aagucuggcg ggaccaacca gaaaauccag uugacgccga ggggacgccu gcauaaggaa     3060

acaguguaug gcaaauuaca acaguacgcu accaaggagg agaagguuaa ugccuccuuu     3120

acugaagacu acauucagaa aguggcaaag aaagaguauc gggaagcgcu ccugaagaga     3180

cuugaggaaa acgauaauga cccuaaaaag gccuucacag guaagaacgc ucugaauaaa     3240

acccccaucu acauaagucu aaaggauaac auuaucgucc cagagaaagu gaagacugua     3300

uggcucgaga ccgacuauac aaucaggaaa gauauuacgc cugaccugaa gauagacaag     3360

gugaucgaug uugggauuaa acgaaucuug caaaaucgcc ugaacgaauu uaauggcgac     3420

cccaagaaag ccuucgucaa cuuaggagag agcccaauuu ggcucaauaa ggaaaaaggc     3480

aucgcaauaa agcgggugac caucucuggg guguccaacg cucaggcccu gcacacuaag     3540

aaagaucacc uuggaaacga gauucuggac aagaauggua accccauccc ugucgauuuu     3600

auuucaacag gcaauaacca ucacguugca aucuacagag acgaaaaagg gaauuugcag     3660

gaggaggugg ugaguuucua ugaugccgua auaaggcgua accucggacu gagcgucauu     3720

aauaagaacc augaaaaagg cugggaguuu cuauucucga ugaagcagaa ugaauuuuuc     3780

aucuuuccau ccgacgguuu caacccgcac gagaucgauc ugcuuaaucc caacaauuac     3840

caucucauuu cuccuaacau cuuccgcgug cagaaaauaa gcaccaagaa uuauauguuu     3900

aaccaccacc uggaaacaaa ggcuguugac ggggagaugu ugaaaucaaa gaaagagcug     3960

aguaagacuu ccuaccauuu cauucaaacc ccaacgaacu uaaauggcau cauuaaagug     4020

cggaucaacc accucggaaa gauaguccau cugggcgaau acuga                     4065


<210>  3041
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3041
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cuucauaaau       60

gaggcugaaa acgagaucga aguguccucu auugucaaua cagguguuag aaucgugccc      120

cugacuacgg augaggaauc agacuuuaag aaaggcaaca ccauuaguau caaugcagau      180

aggacauuga agcgcggggc ccggcgagga uuacagcguu ucaaacaaag aagggaggcg      240

cugcuagaga cuuuuaaccg gauaaaguuc aucgcuaccg acuuuguaua cgccgaaaca      300

ggcgcagcca ccacuuucag cuccuaucag cuccgcgcua agucugcagu ggaggccguc      360

acaaaagaag aacuggugaa gguucuucug auguugaaua aaaagagagg uuacaaaucg      420

ucaaggaagg cuaagaccgc cgaggaaggg gaugcgauug acggaaugaa aaucgcaaag      480

gagauuuuug aaaacaaucu cacgccuggc cagugggugu auaacagccu gaguaaaggc      540

gggaaguuca ucccagauuu uuaccacucc gaccugcaga aagagcucga aaagauaguc      600

cgguuccaaa aucaguuuca uucugagcag auuaacgaua aauuacuggc cgacauccag      660

ggaaagagcc gcacucaaac cucccagcac uucucaaaga cacuuaauau uccguuggcu      720

gagaacaaag guacucgaga agagaccaag cugcagcauu aucaguggag aaaugaagcc      780

cuagcaaacg agcucgauau gaaaauccug gcuuuuauca uuacagaaau aaacaaucaa      840

aucaaccaga guagcggcua ccuuggggcc auuucugaca gguccaagga gcuguauuuc      900

aaucaggaga ccgugggaca auaccaguac aaacaguugc aggaaaacgu acacgcgaag      960

cucaagaauc aaguuuucua ucggcaggau uaccuggacg aguuugagaa aaucugggag     1020

acgcaggcca aguuccauuc agaauuaaac gagaaacuga aggcagagau acgugacauu     1080

acuaucuuuu aucagcgcaa acucaagagc caaaagcacc uugugaguuu cugcgaauuu     1140

gagaaaggcc acaaggcuau ccccaaaucu uccccucugu uccaggaauu uagaauuugg     1200

cagaauuuga acaaugucgu ggugaagaac gagacaacca aagaggucuu cgaacuaagc     1260

gaggaacuga agcagcucau cgccgaggaa cugcaauuua aggagucgau gacugauguu     1320

cagaugcuua cauucuguga gcugaaaaag gguacccaua cggugaauuu uaaaaagauu     1380

gaagggaaca ggaccaauac augcauauac aaggcuuucg agaaaaucuu ggaaguagag     1440

ggauaugaaa uggacuuuuc uaaaaugaag ccacucgaga uuaaggagac uguggccccc     1500

aucuucgaaa cccugaacau caauacaguc auuuuagauu ucgacccuuc cauacagggc     1560

aacgauuuug acaaacagcc auacuaucaa cuguggcacc uacucuacuc agcagaggau     1620

gacgauaagc ugaaagaaac ucuuaagacc aaauucggcu uuaaggagaa ccauuugccc     1680

uuccugcuca auaucaaccu gcaggccgac uacgggaguc uuagcgcuaa ggcgauuaaa     1740

aagauccugc cucacuugau ggauggacac auuuaugaca aagcaugucu cauggugggu     1800

uacaaucauu ccucuagccu gacaaccgcc cagaacaaug accggguuuu aaaggauacu     1860

cuggaacuac ucaaaaagaa cucacugcga aauccagugg ucgagaagau ccuuaaccaa     1920

augauaaaug ugaucaacgc uauuaugaaa gacccggcca ugggcaagcc cgaugagauc     1980

cgcguagaau uggcaagaga gcugaaagcc aauaacgaac agaggaagaa agcuacgagu     2040

gacauuaaua cagccaccac ugagcacgag cggauccgca agcuccugca uuccgaauuu     2100

aacauaccua gaguuaccag gaaugauauu auccguuaua aguuauggcu ggagacaggg     2160

ggaauuagcc uuuacaccgg caacccaauc aaagguucug acuuguucuc aaaggauuau     2220

gacauagaac acaucauucc caaaagucgg cucuuugaug acagcuucuc caacaagacu     2280

cugugcgagc gacagcuaaa uaucgauaaa ucuaacaaga cagcauacuc guuucugcag     2340

gaaaaacuca gcgagaauga cuucgcggcu uaugagaaca gagugaagga ucuguuuaag     2400

acggggaaaa uuaaguccua caccaaauac aagaaacuuu ugauggccga caaugaaauc     2460

ccugagggcu ucauagacag gcaacugcgc gaaacucagu auauugcaaa gaaggccaaa     2520

gagaucuuac ucgaagucuc acggaacgug acagcuacca ucggaagugu cacagauaag     2580

cugagagagg acugggagcu uguggauguu augaaagaac ugaauuggga gaaguacgac     2640

aaauugggcc ucacucauau ugaagagggg aagaacggag aaaggcuguc uaagaucaaa     2700

gauuggacca agcgcaauga ccaccgacac caugccaugg augcaauuac gguggcuuuu     2760

accaaaccag ccuauauaca guaccuaaac aaucugaacg cgaagacaca gggugagaau     2820

aaagccaaca gcaucauugg caucgagaau aagcaccucu aucgggacaa gaacaacaaa     2880

cugcguuuca uuucccccau gaagaauuuc agagaagagg cuaaaaagca acuugaauca     2940

aucuugauaa gcuacaaagc aaagaacaag guagucacua aaaauaagaa caccacuaaa     3000

aagucugggg gaacaaauca gaaaauccag cugaccccga ggggccgcuu acauaaggag     3060

acgguguaug guaaacucca gcaauacgcc acaaaggagg aaaagguuaa cgcuuccuuu     3120

accgaggauu acauucagaa aguggccaag aaagaauauc gggaggcacu gcuuaagaga     3180

cuggaagaga augacaacga uccuaaaaag gcguucacug ggaagaaugc ccuaaacaaa     3240

acacccaucu acauuagucu caaggacaau aucauagucc cagagaaagu gaagaccgua     3300

uggcuggaaa cugauuauac cauuaggaaa gacaucacac cugacuugaa gaucgauaag     3360

gugauugacg uuggcauaaa acgaauccug cagaaccgcu uaaaugaguu uaacggagau     3420

cccaagaagg cuuucgucaa ucucggcgaa agcccaauuu ggcugaacaa agagaaaggg     3480

aucgccauua agcgggugac gaucucugga guguccaaug cacaagcccu ucacaccaag     3540

aaagaccacc uggguaacga aauauuggau aagaacggca aucccauccc ugucgacuuu     3600

auuucaacug ggaacaauca ucacguugcu aucuacagag augagaaagg aaaccuccag     3660

gaggaagugg ugaguuucua ugacgcagua auuaggcgua aucuggagcu aagcgucauc     3720

aacaagaauc augaaaaagg cugggaguuu cuguucucga ugaagcagaa cgaauuuuuc     3780

auauuuccau ccgaugguuu caauccgcac gagauugacc uucucaaccc caauaacuac     3840

caucugaucu cuccuaauau cuuccgcgug cagaaaauua gcacaaagaa cuauauguuu     3900

aaucaccacu uggagaccaa ggccguugau ggggaaaugc ugaaaucaaa gaaagaguua     3960

aguaagacau ccuaccauuu cauccaaacu ccaaccaacc ucaacggcau aauuaaagug     4020

cggaucaauc accugggaaa gauuguccau cuuggcgaau acuga                     4065


<210>  3042
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3042
auggccgacu ucgcuaagua ccagcccggc augguggcag augggagaaa aaucccucug       60

gacuggaccc ucuauuaccu uaggaagaaa gcccugacac aagcgauucg gaaggaggaa      120

uuggcuugga uauuacugaa cuuuaaucag aaacgcggau auuaccagcu acgaggugag      180

gaagaggaag agaacagcac uaagcacgag gaauauuacg agcucaaggu cguuuccgug      240

gaagccgcag aggagggcaa agggggaaau acgugguaua acguaacccu ggaaaauggc      300

uggaucuacc guagacaguc uaagauugag cuuaacgauu ggguggguaa agucaggcca      360

uucaucguga caacugaaua cgagauugac gggguuacca ucaagaaaga uaagaaugga      420

gaagugaagc ggucauuucg cgccccgagu gagaacgacu ggggccugag aaagaagagg      480

acagagagcu ugcucgaaga guccggcaaa accgucgggg cuuucauaua uaaucauauc      540

cugucugaac ccgcagauaa gauucgggga aacuuuaucc gcacuauuga gcgaaaauuc      600

uacaagaaag aacuggaggc cauccucaga gagcaaucga agcaccagaa ggaauuaaaa      660

gacauagaua cacugauugc uugcaucaag gagcuuuauc cuaauaacaa accacaucag      720

gacucauuga uuaagaaaga uaugaccuac cugcuaauca cggaccucau cuuuuaucag      780

aggccccuua agagcaagaa aagucuuauu gccgauuguc cuuacgaaua uuacgaguac      840

gugauaaagg aaacugguga gaauaaacgg caauccauca agugcauugc gaaaucuaac      900

ccauauuacc aggaguuccg ucuguggcag uuuaucauaa auuugcgccu cuucgacaga      960

aacaacgaca aggaaguaac cagcgaguau cuguccucaa uggaagauua cacaagguua     1020

uuuacuuauc ugaaugaccg gaaggauauu aaccaggaga cccuccuuaa agacuucuuu     1080

aagcaaaaga agguuaaacg aggcauggag aaggaguucc ccauccgcug gaauuacauc     1140

gaggauaagg aaaagaagua uccuuguaac gagacaagac acgaacuguu gaccgcacua     1200

gggaaagugg gaauugacca uaguuggcug gaugacaagg aacucuucua caggcugugg     1260

caccuucugu acagcgucga gucuaaagaa gaggccgaag gcgcuuugcg gaagcucgug     1320

gauaaugacg auuuugugaa cgccuuccug aaaaucaagc cauuuaagaa agaguauggu     1380

gcauacuccg aaaaggcuau uaaaaaguua cuggccguca ugcgcauggg gaaucuaugg     1440

aacgagguug acauaugcga gcagacgaga gacaggaucc agaauauuau caaaggagau     1500

aucgacgaaa agauuaagga ucguaugggc cggacuuucu cacaacucag cgacuuucag     1560

ggccugccgg aguggcuugc guguuaugug guauacgggc agcacaguga agccacagau     1620

auacagcgau ggaccaaacc cgagaacuug auggcauaua ucaauggauu caagcaacau     1680

ucucugagaa acccuauugu ggaacagugc auccucgaga cucugaggac aguccacgac     1740

auuuggaaag aggcugguca uaucgaugaa auacacaucg agcuuggccg cgggaugaag     1800

uccaccgccg accagcgggc uagaaugagc aaaucgauua ugcagaauga aaacacgaau     1860

cugaggauca agucuuugcu cauggagcug aagaacgcca aagaguuuga aaacgugcgc     1920

ccauacuccc ccaugcaaca ggagauuuua cgaaucuaug aagagggagc acugcaggaa     1980

cuaaccaagg aggaugccca guacaaugag auaucaaaaa uuagucaaau ggcucagccu     2040

agcgcgaacg aacuccugaa guaccggcuu ugguuggagc agaaauauug uuccccauac     2100

acaggcaagc ccaucucucu gagcaaacuc uucacuucag cauaucagau ugaacacauc     2160

auaccgcaaa gucguuacuu ugacgauucc uucaccaaua aaguuaucug cgaggccgaa     2220

gugaaccagu uaaagagcaa uaugcugggu uaugaguuua uuaaaaagca ugggggcgag     2280

aucgucagau guacaaugau uggagacgug aaacugcuug augcuaagga auacuauaac     2340

uucguaacug agcacuacac caauaacagg cgcaaaaagg aaaaguugcu cauggaggac     2400

aucccucagg aauuucugaa ucggcaaaug aacgacucua gauacauaag caaaaagguu     2460

auggccauuc uaaguaauau cgugagggag gaggaugaac aggaggcaaa aagcaaguuc     2520

gucaucgccu gcuccggcgg gauuacagac cgacugaaac aggauugggg acucaacgac     2580

guguggaacu cuaucgucua uccccgcuuc gaacggcuga auaaacuuac caauacugag     2640

aacuuuggua gaugggagaa caagaaaggc aaacaggugu uccaaacguc gguuccauug     2700

gaacugcaga aagauuuuag caagaaaagg auugaccauc gucaccaugc uauggaugcc     2760

uuagugauag caugugcguc caauaacauc auuaauuacc ucaacaauga gucagcuaag     2820

agucaggaaa agcgcgagga ccugcggaaa aagcugugcg auaaaaacag aaucauuagg     2880

aagccuuggg aaacauucac ccaggacgcc cacacugcac uggagaaugu agucgugucu     2940

uuuaaaaacu auauccgagu uauaaauaag gccaccaacu acuaugagaa guacgauaaa     3000

aaugggaaga aagaaaucgc ugagcaaaag ggacaggaca ugugggccau ucggaaaccc     3060

augcacaagg aaacaguguu cggccagguc aacuugcgca gaaagaucac cauuaaacuc     3120

aaggaugcac uggagaaaau cccagcuaua ugcgacaagg aacuaaggga cuauauuaau     3180

auccuggugg ccaaacaguu uaacaagaaa caaauccucg cgcauuucaa gagcauuaau     3240

uaccggcuga acaugaaguc cguagauaaa guggacguuu ggcaguuuuc aaacgagaag     3300

gagcccaugg ucgccacucg aaaaagcgug gauacaucuu ucgacacgaa gagaauagcu     3360

accaucacug auacagguau ucagaaaauc cuuuugaauu accugaaggc aaagaacaau     3420

gacccuaaca uugccuuuac cccagaaggg aucgcugaga ugaauaaaca cauaacagaa     3480

uauaacaaug gcaagcaaca ucagccgauc uuaaaagugc gcgucuccga gcccaaggga     3540

gccaaauacc agguuggcga aacugggaac aagaggagua aguucgugga ggcacagaaa     3600

ggaaccaauc ucuauuuugc gauuuacgag gauaaggaag guaaccguag cuauaauacg     3660

aucccucuga acgaggugau ugaacggcuu aaacaaggcc ugucuccagu acccgacgcc     3720

aaugagaagu ccaucccucu aaaauuccac cucucaccaa acgaucuggu cuacgugccc     3780

aagaccgacg aggaaaauga gauaaagaug gggcagauuu auaaauucgu ugauaguagc     3840

ggaacaacug cuaacuuugu gccgcacaga gucucgaauu ugaucuacaa cguggacaag     3900

aaaaucgccg aaaacuucug uaaucaugag auuauccaga acgaauacgg cauagguucc     3960

ccucagucua agaaucaaaa aucaauuacc ggggagauga ucaaggcagu uugcuggaaa     4020

cuggaggugg acagguuagg caacauucac aagaucauaa aguga                     4065


<210>  3043
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3043
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggaugggc cuucauaaag       60

gagcccgaaa aagauguggu cgguuccgag aucguugaca ugggcgugag aauuguaccu      120

cugucuucag augaagagaa cgacuuugcu aaggggaaua cuaucaguau uaacgcagau      180

aggacguuga aacggggagc ccgccgaaau uuacagcguu ucaagcaaag aaggaacgcg      240

cugcuagaaa ccuuuaagga gaaaaaucuc aucccaagca acuuccagua cgcugaggac      300

ggcccguccu cuacauuuuc gacucugaaa cuucgggaua aagccgcaaa ggaaaagaua      360

gagcuggacg auuucgugaa ggucuugcuc cagaucaaua agaaacgcgg uuauaaguca      420

agcagaaaag ccaugaguga agaggacgag gggaccgcua uugauuccau gggaaucgca      480

aaggaacugu acgagaacga ccugacaccc ggccaguggg uguaugaagc ccucaaaaau      540

ggcaggaaga acguuccuga uuuuuaccgg ucugacuuac aagaggaguu caaaaagauu      600

gugaauuauc agagcgaauu uuacccagac auccugaaca ccuccuucgu cgaggauugg      660

auggggaagg cuucaacucc cacaaaacag uauuuuaaua agcgcggagu gcagcuugcc      720

gaaaacaaag guaagcgaga ggaaagaagg uugcaagagu acaaauggcg ggcggaagca      780

guaaauuuca agauagaccu gagugagauu gcccuaaucc ucagccagau uaacucucag      840

aucuccaacu caagcggcua ccugggggcu aucagugauc guucuaagga gcuuuauuuu      900

aaaaaucuga ccguuggaca guacuuguau caacagguga agaaaaaccc ucacacgcgc      960

cucaagggcc aggucuucua cagacaagac uaucuggaug aauuugagaa aauuuggucc     1020

gugcagagcu cguucuaccc acaguuaaau gacgaacuga agcaggaggu gagggauguc     1080

acuauauucu uucaacggcg acucaagucu cagaaacauc uuaucuccaa cugcgaauuu     1140

gaggagcacc auaagguugu gcccaaauca cacccuauuu uccaggaguu ccgcaucugg     1200

cagaaucuga acaauuugac ccuaauaaag aaagacaacc ugaaugaaaa gugugaacuc     1260

gcccuggagg auaagacagc acuugcuaac gagcuggccu uuaaaaagga auugagugua     1320

aaagacgcgc ucaaggugcu ggguuuaaaa ccaagcgauu gggaguucaa uuuuacuaag     1380

auugaaggga acagaaccaa ucaugccuuc uuugaggcau ucgcuaaaau caucgaacug     1440

gaggacggag agccgauuga ccuaggcaac cucaaggccg augacauccu ggaucaauuu     1500

uccgaagcau uccuuaggau uggcauagac acagaguugc ugcaggucaa uucugauauc     1560

gaaggggccg aguaugaaaa gcagagcuac auucaguucu ggcaccuccu guacucaagu     1620

gaggacgauc aaaaacuuaa gcagaaccug auccggaaau uuggauucaa gcccgagcac     1680

gcaaaaaucu uggccuccau uagccuccag gacgaucaug cuucucuguc aagucgcgcg     1740

auaaagaaga ucuuaccuca ucugcagagc ggucuaauuu augacaaagc augcaccuac     1800

gccggcuaua accacuccuc uucguuuacg aaggaugaaa augagaaaag agaacucagg     1860

gcugagcugg aacuuuugaa gaaaaacagc cugcguaauc cagugguuga gaagauccuc     1920

aaccaaauga uuaauguggu caacgccauc cugaaggacc ccaauuuagg gcggccugau     1980

gagauacgag uggaaauggc aagagagcug aaagccaacg cugaacagag gaagaauaug     2040

acuuccaaca ucgccucagc aacacgcgac cacgagaaau accgggagau ucuuaagaaa     2100

gaguucggau ugaagagagu aaccaagaau gaccuccuga gguauaaacu auggcuggaa     2160

acugauggca ucagucucua cacagguaag ccaauugaag cgucuaaacu guuuagcaag     2220

gaguaugaca ucgaacacau aauucccaaa gcucgccuuu ucgaugacuc cuuuucaaac     2280

aagaccaucu gugagcgaca guugaauauu gauaaagcca acguuacggc auucagcuuu     2340

cugcaaaaua aguuaucugc cgacgaguuc gaacaguacc agucccgggu gaagagucuc     2400

uacgggaaac ugagcaaggc uaaaauccag aaacuucuga uggccaacga uaaaauaccg     2460

gaggacuuua ucgcacguca auugcaggaa accagauaua uuucuaagaa ggcuaaagag     2520

auccucuucg aaauuacaau gagggucucc gugacuaccg gcacaaucac ugauaagcug     2580

cgcgaggacu ggggccuagu cgagauaaug aaagaacuga acugggagaa guacgauaaa     2640

cucggacuga ccuauacaau ugaagggaag cauggagagc ggcuuaauaa gaucaaagac     2700

uggaacaaga gaaaugauca caggcaucac gccauggacg cguugaccgu ggcccugacu     2760

aaaccugcuu acauccagua uuuaaacaau cucaacgcaa aggccauugg uaauaaaaag     2820

ggcacggaaa ucuuugcuau ugagcagaag uaccugaaac gagagaacgg gaaacuuugc     2880

uucauacccc caaucgaaaa uauucgcgcc gaggcuaaaa agcaucuguc aaaaauccua     2940

guuaguuaua aggcgaagaa caaaguggua acaauuaaua agaacaaaac caagaaaaag     3000

gccggauuca augaacaaac ugcucucacc ccucggggcc agcugcacaa agagacaguc     3060

uacgguaaga gcuuucacua cucgaccaag uucgagaaaa ucggggccuc cuuugacgca     3120

cagaagauaa acacuguggc uaaaaaggaa gagagagaag ccuugcugaa aagguuagca     3180

gagaauggca acgaucccaa gaaggccuuc acaggaaaga acucucucaa uaagaugcca     3240

aucuaucugg accuuggcaa aaacauuaag cugagcgaaa aaguuaagac gguggucuug     3300

gagcagaauu acaccauccg uaagaacauu gaucccgacc ucaaagugga uaagguaauc     3360

gacgugggga uaaaacgcau ucuggaguca cggcuagaag aguuuggagg uaaugcuaag     3420

cuggcguuca guaaccuuga agagaauccu aucuggcuca acaaagaaaa gggcauccca     3480

auuaagagag uuaaaauauc cggggucucu aauguggaga gccugcaugu gaagaaagau     3540

cacuuuggag agaagaucuu ggacgaagag ggcaacgaaa uuccggucga uuucguuucc     3600

acugguaaua accaucacgu ggccaucuau gaggacgaag augggaaucu gcaagacgag     3660

gugguaaacu uuuacgaggc agucauuagg cgaaaucagg gcgaaucaau cauaaaauau     3720

cggcacgagg aaggauggaa cuucuuauac agcaucaagc agaaugaguu uuucguguuc     3780

cccagucaag acuuuaaccc uaacgauuuc gaccuccugg auccaaagaa uuaugaccuu     3840

auuucuccca accuguaccg cgcucagaag uuuuccaagg uuauguacgg caauagcgcc     3900

gugagagauu augucuucag gcaucacuug gagacagugc ucaacgacgu uaaagaacug     3960

aaggaugugg ccuacaaaca gcuaaagucg cugcaguauc uuaaugggau caaaaaggua     4020

cggcucaacc aucugggaca aauuauccag gucggcgagu acuga                     4065


<210>  3044
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3044
augacccugc acuacaacgu gggccucgac aucgggacaa auagcgucgg auauacuauu       60

guugaugaca acgguaagau acuucaggug aaaggcaaga auggguacgg aguaagagug      120

uucaaagagg gcgccacggc ugcagaaagg cggaucuuuc gcaccacaca ucgacgucug      180

aagagaagga aauggcgguu gcgcuuacug caagauuucu uugagcccua uauucuaccu      240

caggacgaug guuucuuuau cagaaggaag gaauccaacc ucguccugaa ugaccgggau      300

gaggacacug ccucucuuuu caacgaucgc ucagaccgag auuuuuacca ggcguaucca      360

accauuuacc accugagaca ggcuuugaug acagaaaaga ggcaauucga cgugcgggag      420

aucuaucucg ccaugcauca cauaaucaaa uaccgugggc auuuucugcu gaauggaacc      480

guuaacaauu ucacuaacaa uaagauugau cuccacagug acuuugagga auuaaacgca      540

cugcuuuuga augugauccc ggagcugaaa uuccagauug accuagauca cagcacaaug      600

uuucagacca cgcuccugaa cacuaccauc ucccgcucug gcagacagag ggaccuuguc      660

aagucguucu acaauggcuc agaugacaaa caacuggcca agcagcagaa gaguguggcu      720

acagaauugc ucaaagcaau acugggguua aaggccaaau uccauaagcu guuuaaccuc      780

gaucuucugg agacuaccga gugggaguuc aguuuugacg cugaagauau ugacgauaaa      840

uuggccaagc uagaaccccu gaugacagag aaccagcaac agguaaugga cauccuccag      900

cggauuuucg cggcaaucac ccugaaugga aucguuccug aagguaaaac gcuuuccgcc      960

ucuaaggugg auagcuauca aaagcacaaa caggaccuga aguugcucaa agcuauugcc     1020

cagcauuccg acaagaaaac ugcaacaguc cugcagaccg cuuacaacga guauauaaau     1080

ggcaucgccu caaagccauu aaagcaagag gauuuuuaca aacgacugac uaacgcggug     1140

aagaaaagua gcgaccccca gguggccgau augcuagcac ucauugacca ggaaaaguuc     1200

cugccuaaac agcgcacaaa ggagaauggg gcuaucccac accaacuuca ccaguuggaa     1260

cuggagagaa uaauugauaa ccagaaggac uauuaccccu ggcucgccga acugaauccu     1320

aaccaggcua ggcaaaaagu cgccaaguau aaacuuucug agcugguugc auuucgggug     1380

ccauacuacg uaggaccguu gaucgagccc gaagugcagc aggccaccuc caaugcucau     1440

uucgcgugga uggcacgcaa ggaggccggc ccuaucacgc cauggaacuu ugaucagaaa     1500

gucgacagaa augugucagc ugaaagguuc auuaagcgua ugaccacaaa ggauacuuau     1560

cuccuggccg aggacguuuu accccugcac agccuacucu accaacgguu uaucgugcug     1620

aacgaacuua auaacguccg agugaauggu cagaaauuga ccaagaaaca gaagcaagca     1680

guauaucagg aucuguucaa aagacagccu cauguuacaa agaaacaguu uaagaguuac     1740

cucgugcaaa cuggggaguu cgccgagucu uccaagauug aaggacuggc uaacgagacc     1800

agcuucaacu cgggcuuaac aaccgaaaau gagcugagga aaauauuugg cgcccagcuu     1860

gacgaggcac gcuaucaggu cgauuucgaa cagaucauug agugggcgac uuuguuugaa     1920

gacgcuaaga uccuccggac gaaacuggcc gagaucacau ggcuaaccac ugaacaaauu     1980

gagaagcugg cagggauaag auacagggga uggggucgcu ucucucgaaa acuccuggcc     2040

ggccuucggg accagaacgg gcagcagauc auugauuugc ugugggacac cccaaauaac     2100

uuuaugguga ucgucuccca agcugccuuc ucagaggcaa uuacaaagga aaaugagaag     2160

uuaaucgauc guagaggagc ucaggacgug auaaccgauc ucuauacuag cccccagaac     2220

aaaaaggccc ugaggcaagu ucuugcgauc guggccgacg uacagaaagc uaugggcggu     2280

gucccgccuc agcgcauuuu uaucgaguuc gcacgggaag augaaaagaa ucccagaagg     2340

agcguggagc gaucccgcca gcuggagaaa uuguaccaaa caauuucuaa cgaauuucuc     2400

aucaauagcg agguucggca ggaacugaag gaggccgugg accagaaggu caacuucaaa     2460

gauagacuau uucuguacuu ccuccaaggg ggcguggacc uguauucagg aaagaggaua     2520

aauauugauc agcuuaguca cuacgacauc gaucauauuu ugccacaguc cuuuaucaaa     2580

gacgacagcc uggauaaccg uguauuagug ucucagaagc ucaaucgcuc aaaaagugac     2640

agcguuccuc uguccgaguu cgcuaacuau aaguucggcc ccgccaugca ggcaaagugg     2700

cuucaacuga aagaagcggg gcuacucucu aagcggaaau acgauaaucu gacguuggac     2760

ccaaccaagc ugucgaaauu ugcccccuua ggauucguca acagacagcu cguggagacu     2820

cgacagguga uaaagcuggc ugccaaccuu cuggcaagcg aagagacaaa agucguuacc     2880

aucaaggcua auaugacaca ccaggugagg aaggaauugg auuuuccuaa aaaccggaau     2940

gugaacaauu aucaccaugc cuucgacgca uaccucacug ccuuuguagg uauuuuccug     3000

uccaagcgcu auccaaaacu aaagccguac uuuaccuacg gcgauuucca aaaagggaac     3060

aagcugcccg accuuaagaa uuuuaacuuc cucuaugagc ugaaaaauaa ggauagauca     3120

aucgacagua acacgggaga gauuaucugg gauaaacaga gggacuuggc uuacaugaau     3180

aagauauaua acuuuaaaaa ggucaccgug guucacgaag ugcugacaaa gucuggcgcg     3240

uuauacaauc agacucucua uaaagccagc gaggacaagg cauccggucg ggggaccaaa     3300

caacugauuc gaaagaaaga uaacaugccu acagaacuuu acggcggaua uacuggcuca     3360

accagcgcuu ucaugucuau cgucagacug uggaagaagg acaaaccaua cuacaaggug     3420

guugggaucc ccacgcgcau ggccgccaaa uuggcuaauc aggcacagcu ccuggauuau     3480

cuaacaaaga aauucaccac uaggaagcug gugaagaaaa caggagacua caagaccacu     3540

guagagcguu uugaacuugu cgugccuaaa guggguuuca accagcucgu cauugauggc     3600

gggcaaccau uuaugcuggg auccgccacc uaucaguaca acgcgcggga guuguucauc     3660

aguaaggagg cuguuaaagc cuuaaauaag cagcugagca cacaugugga ccucguacag     3720

guguuugaug aaauucuggu ccaagugaac agaugguucc cccuuuauga cacgaauggc     3780

uuuagggaga aacugucuca cggucgcgau cgauucauga aguugaacga cuccuuugaa     3840

gagaagucag auacccagau agacguucua aauagaaucc ugauuggguu ccacgcaaac     3900

gccgcucgga cuaaucucaa aauccugggc cuuggaacag auuuaggcuu uaugacccag     3960

caggcaggga uuaggcugac agaaaaggcc auccucgugc aucaaagucc gagcggauug     4020

uucgagcgca aagucgcgcu gcgugaccuc aagggccuga gauga                     4065


<210>  3045
<211>  4068
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3045
augaccuaca gcaucggccu ggacauuggg acaggauccg uggguugggc cgucauaggc       60

gaugacuaua gacucaagag ggcuaaaggg aagaaccuua ucggaguucg gcuguucgau      120

ucugcaacua cggccgcgga gcgccgaggc uaccguacca caagaaggcg guugucacgc      180

agacacugga gguuacggcu gcuaaaugac auuuuugcua gugaucucgc cgaacaggac      240

gagaacuucc ugccccgccu uaaauauagc ugggugaauc cugaugacga acaaaaccca      300

cagcuuaauu ccgauguagc aaacggugcc uuguuugggu cuucggagac ugacaaggcu      360

uuccauaaaa auuacccgac caucuaucac cuccgacaug aacugaugac agauaccgca      420

aagcacgacc ugagagaggu guaccucgcc auucaucaca ucgucaagua uaggggaaac      480

uuuuuaaaua cugcugauaa aauaaacgcc gaaaauucau ucaacgugga ccuguuuauc      540

gcggcacuug acgaauacaa ugaguucuug gaaacaccca uuaccagcau caaggaugcc      600

cagcuguuua gugacaaacu aacggauauu aacgcucgga auaaguccgu ucguguggag      660

aacgccaugu cugagacuua cgacgauaaa agcaagaucu ucaaggcaau acucaccgcu      720

cuggucggca acacagccaa uuuucagacu auuuucgacc uuuccgaucu ggaaaaagac      780

gaggcgaaga acuuuaaauu caaguuggau cucgaagacg uggagaccaa acuggaugua      840

guucgcgcca aguuaucaga cgaacaaaau gcauuccugg aggcugugcu caucgccuau      900

gacggcauua cacuuaaaau gauccugggg gauuacaaga guaucagcca gucuaugauu      960

gaguccuaua agucacacca gagagacugg aaauacauaa agaacaaugu cagguugaac     1020

gaacagaaaa ucgagccuga uaagaauagc gugaaauuuu ucaagcuaga cccaaagaaa     1080

cugauuuauc ccaguaaccu cgcuuaccug caagugaucu cuuccaauga agaugagcau     1140

cggaagggac uugacuauuu uaaaaccgcc auacaggaug cagacauucu gcagcgaauc     1200

gaaaacgaua cguucuugcc ucgccagaga acuaagagga augguguccu cccauaccaa     1260

cugcacuuag ccgagcugga gcagaucauu aaaaagcaga gcaaguacua ucccuuucua     1320

gcuaacaucu acacacaaga caauaaaacc gaaaacaagc ucauuggccu gcuuaaauuc     1380

cggguuccuu auuacguggg gccauuggua gaggcggaaa aggugacugg agauggcaaa     1440

aaucauuggc ugauaaagaa ggagcuccag gaaaacacac cgaucacccc cuuuaacuuc     1500

gacgaugucg uggacaaaga caagucgggu gagcaguuua uuaaacgcau cacggggacc     1560

gauucuuauc ugaucggaga gccuacacuu ccagaaaauu cccuguugua ccagaaguau     1620

aacguucucc aagagcugaa uaacgugaga aaugaaaggc accagcguuu acccauuaaa     1680

gucaagcagg acauauucga gcacguguuu aaaaucgaua agaacguaaa ugcaaagaaa     1740

guuauggccu accugaagcu aaacggcuac ggcgacuucc agauugcugg gcucucagau     1800

acucggaccg gaaaauuuaa uaguaaccug agcuccuaua auuacuucgu ggaccuuuug     1860

ggucgagaga agacagaaaa gaucagagag gauaggcugg aagagauuau cgaaauacaa     1920

acugucuucg aggacaagau ccucguggcc aagcgccugu cuaacuuaga uuuucugacc     1980

ccucaggaga aaagcaagcu gucagcaaaa cauuauacag gcugggggcg guugagucag     2040

aagcuccuga ccacucaguu cauuaaaacg gcccuauccg aagcuggaga caucauucca     2100

caaagccacu cuaucauaga ucugcucuac uauacagaca agaaucugau ggagauucuu     2160

aacgauaaga ccuuuaaugu cagaaacugg uugacugaac agaacaaagg caccgaguca     2220

gguaauaguc uguacgacca gaucgacgcc uuagcagggc ccaaggauau uaaaaggggc     2280

aucacacagg uguucagcau aaucgacgau auuaagaaag cgaugggauc caacccggaa     2340

accguuuauc ucgaguuugc ucgcgagacu caaacaucuc gacugaccaa uucgcggcuu     2400

aaccgugugc aggaaaucua caauagcccu gagcugaaga cggaguucgc cuacuuggca     2460

aagaaacucg aggccgaaac uaaggagucc auucaugacg auagacugua ucuauacuau     2520

cugcaggagg gcagggacau guacacaggg aaagaaaucc ccuuugauca aauaucaagu     2580

aacuaugaca uugaucacau caucccacag gcuuucacca aggacgauuc ucucgacaau     2640

cgcguacugg ucaaccgggc cacaaaugca agaaaaagca acuccccuac uuuuaccaau     2700

gacauuaucg auaacaggaa gucauggugg cagagccuug ugaaacgagg auugaugucu     2760

aaggagaagu ucgaacgccu gacggacaaa cggaagaccu uuuccgagaa ucagacaaac     2820

cauuucauug cuagacaauu aguugagacu aggcagauaa ucaaaaaugu ggccgaucuc     2880

auuaagaaca acuacgacgg uacccaggcg gucgccaucc gugcuagucu gacaggcgaa     2940

augcgccggu aucuugauug gcccaaaaau agagacauga acgauuacca ccacgcacau     3000

gacgcccuga ugauggcuac uguggggaag uacguagaua ggcgaggauu uuucgacaau     3060

ggcagcgugu cugauggugc cgggaacgca uauaaucuau acaccaagga cuggcucguu     3120

gcggcccggg ucggcacgcg caacgauucc cagagaguga auccauaugg auucauugug     3180

ggcucaauga acaguaaagc ugucaggcaa cagguuaaua aggagacagg ggaaaucgug     3240

uggggaaacg agaaucugga cuacuugcgg cacgugcuga gcuauaaaaa gauauuagua     3300

acccgaagac ucucggguuc cucuggccgc cuguacgccg aaacucuuuu uagccccaac     3360

ccuaaagcaa agcugaucgc ucagaaggcc aauaggucag ucgcauugua ugggggauuc     3420

acaaaagccg agagugcuua caccgcgcuc guggaguuua acaagaaagg cgaccguucc     3480

uacaagcuga uuaaaguucc aauggccauc gcaaaucggg ugaacgauaa gcagauuacu     3540

gucaccgcuu auaucgccuc ucuaaaucug agagacuuca gcaggguggu ucuugaacau     3600

cucccgcugg guacauugau acgcgagucc gaugggucag aauuuuacgc cgcuagcagu     3660

gaguauaagc acaacgcaaa agaacugugg gcccccaagg agauuuuacg aucuuccaaa     3720

aagcucgaca aagcgacggc ugauaaccug aucgacaucu ucgauuuucu uaccaaugac     3780

aagacuauua gcaacuucaa guuuuacgag aaagaucugu ugcaccucaa gaaugugaga     3840

gacaaauucg uagaagccuc ggaugagaua aagaucaaag uccugucaga cgugauuuau     3900

gaacuacaua acgaugcagg cuggcgggac ccuuuuaagc acauccaaga gaaaccagcc     3960

uggacaaugc uucaggugaa gggaggcauu aaacugucug aaaaagcugc aaucgucuac     4020

cagaguccca ccggacucuu cgagacaaua acuaucguug gggaguga                  4068


<210>  3046
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3046
augaagaaau ucgaggaaua cuauaucggc cuggacaugg ggaccggaag cguggguugg       60

gccgucacag auuccgcuua caacauuaua aagagacacg gcaaagcacu cugggggauc      120

aggcuuuuug agucugccaa gacugcggaa gaacggcgca uuuuccgaac gucacguaga      180

aggaccgaac ggcgcaaaaa uagacugcau uuguuacagg agaucuuugc ugaggaaauu      240

aguaagaagg acaugggauu cuaucaaagg augaaagaga gcaaguacug gcacgaagau      300

aaaaaggaca ucaacggcaa uaugcccgag cugccuuaug cccuauuugc agaugacggu      360

uucacagaua aagacuacua uaagaaguac ccaacuauau uucaucuccg guacgcccug      420

gcuaccaaca caaccgauaa accggacauc cgccuucugu auuuggcacu ccaccauauu      480

aucaagcacc gagggcacuu ccuguuugag ggcaaaaagc uguccgaagu uaaagaguuc      540

ucuacugcca uugaagcucu cgugcagcag gccgaggaac aggagaucga uuuaaagucg      600

cugacacaag aggaaguacu uacccaguug gagcauauaa ugucagacgc gcagugcacg      660

aaaagcgaua agaagacuca ggugaguaaa cugauugucg gaaccgacaa gcaaaaacag      720

aagcaggaaa aagcaaucgc cgcucuaauu acaggcugua cugugaagcu cuccgacauc      780

uuugccgauc ccgagcugga caaggaagag aucucuaaaa uuugcuucag cgauaauggg      840

uacgaggaaa aggagguuga aauagagucc gugcuugcag acagauuucu guugaucgcu      900

gccgcgaaag gagucuauga uuggaccgug cuccacgaca uucuggguga ugccagguuc      960

uuaucagaag caaagguaaa aacauacgag aagcaucggg acgaucugcg uaagguuaaa     1020

cagcuccuuc gcauggacaa ggagguguau gaagcugucu ucggcgugag ugagaaaaac     1080

gaagccaauu acagcgcuua uaucgggaug gccaagaaaa acggaaagaa gcaaccuaua     1140

gagaaaaagu guucucagga ggauuuuuac aaauuccuga agaaacaguu ggacagacua     1200

uccguggacg aaaugggcca agagauuauc gaugaacuga aggcagagau ggaccucggu     1260

acccuguuuc caaaacagau gaucagggau aaugggguca uucccuacca gcuucacgaa     1320

aacgagcuga augccaucuu ggacaacgcu cagaaguucu auccuuuucu ccuggagaag     1380

gaucaagacg gauacacgcc aguugagaaa auucagaaga uauuccgguu ucgaaucccc     1440

uauuacgugg gcccuuuaaa uacugcgcac caggauaaag gcgggcauuc auggagcgua     1500

cgcaagaaag agggaaagau uuauccaugg aacuucagug aaaugaucga cgaggaagag     1560

ucugcaacaa aguuuaucga gagaaugacc aauaaaugca cuuaccugau uggugaagau     1620

gugcuaccga aggagucccu ccuguaugcc aaauucaacg uccuuaacga auugaauaac     1680

cugaggauag acggcgagcu caucgcuccc gaacugaagc aggauauuua caauaacgug     1740

uuuaaaaagu accaacgggu uacagggaag aaagugaagg acuaucuucg caucaaugga     1800

gucauuagca aagagcagga gcugucgggc uucgaucaga acuuuaaguc uuccuugaaa     1860

gccuaccacg acuucaagca gaucgugggu accgggguac ucucagaaag ugagcaagaa     1920

gacauaguua gagauaucac gcuguucgca gacagccaga agauguuaaa acagaggauu     1980

caucguaagu auccuaugcu guccgauaaa cagaucgccc aacuagcuaa uaagaaauac     2040

gccggcuggg gacggcucuc ucgaaccuuu cuggagaaca uugagagcgu gaauaaggaa     2100

acaggcgagg ucaugaacau cauaacugca cuuugggaaa ccaaugagaa cuugaugaaa     2160

cugcucucag ggaaguauga cuacacaaag cagauugaac aguggaaugc gagacaaaug     2220

aaaaagggag agaucgagua ucaggauauu gaggaacugu acgugagucc agcugucaaa     2280

aggcccaucu ggcagacuau aaagaucgug cgcgaaauua ccaacaucau ggguggcgcc     2340

ccuaaacgga uuuucguuga gguggcaaga gaaccagggg agaagggagu aaggaagguc     2400

ucccgccgaa aucaguuacu ggaccuuuac aaagccugua agaaagaggc ucccgaauug     2460

uaugaucggc ucgccaacaa ggagagcgac aacgcacugc guucugauaa acuauuucug     2520

uacuauucac aaaugggcag augcauguac aguggugacg cuaucgaucu cgacgaacug     2580

uucacaaaua gguaugauau agaccacauu uacccgcaga gcaaggugau ggacgauucc     2640

aucgacaacc gcguucuugu gaagcgggau cuuaauucua aaaagaccga cacuuuuccu     2700

gucucggagg aauauagaaa agccgggaag gcgcugugga gcacguuacu caaaaagggc     2760

cugaucucca aggagaaaua caagaggcuu acacgaaccg agcccuucgg agaagaugag     2820

cuggccggcu uuauugcucg ccaguuggug gaaacucggc agucaaccaa agcaguagcc     2880

caucuccugg aggaggcuua cccagacgcc gaaaucgugu augcaaaggc gagagccguu     2940

agugaguuca ggcacaaauu ugauaugauu aagguccgug aagugaacga cuuccaccau     3000

gcuaaggaug ccuaccuaaa uauagugguc gggaacacau auuacguuaa auuuaccaag     3060

gacgcagcuu gguucgugcg caauaacccu ggacggacuu auaaucugaa aaagauguuu     3120

gagaacgaaa cagugaaaag agguaaugag acggccugga uccccggcga uaacgggacc     3180

auuuguacug uaaagaaaug gaugaggaag aauaacgucc ucuucacacg acgggcauac     3240

gaggugaagg gaggccuguu cgaccaaaau auccuuaaaa aggguaaagg gcagauucca     3300

aucaagggcg gagccgacga acgcuugaga ucuagggaua aauauggcgc uuacaacaag     3360

gcgagcgggg ccuacuuuau gcugguugag uccgacggaa agaaaggcga acggaagcga     3420

accauagagu ucgugcccgu cuauuuagca aaaucacucg aagagagcgu ggagcugaga     3480

cgcagguacu gcgaagagga acuuggucug guuaacccug augugcgucu aucuaagauc     3540

cggaaaaaug cucucauuga cguagauggg uuuaagaugc accuggucgg aagaacagag     3600

aacagguugc uguuaaucgg cgccacucag cucgugcugu ccgaagacga ucagcaacuu     3660

auuaagaaaa ucgguaagua uugucuggac caucgcgaga auaaagaugc cguguugagu     3720

gagaaguacg aacucaccau ggaccuguuc gagaaacuau augauauauu ugucuucaag     3780

cuggaacaga cgguuuacgc ugugaaguug agcaaacagg caaaggaccu caaagaggua     3840

aaggaggaau uucuggccuu ggaugacgag caaaaaugcc uguuacucau ggaaauucug     3900

caccuuuucc aguguucucc acagcuggcg aacuugacca auaucggcgg gggaaagucc     3960

gcuggcucac ucaaaauccu gagugacauu acaaagcagc aaagcgugcu acugauaaac     4020

cagucgauca cuggguuuua ugagcagguc auugaucuua agaccaucug a              4071


<210>  3047
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3047
auggaggugg gcaacuacua ucuggggauc gacgccggaa ccgauagcgu cgguugggcu       60

guuacagaca aucaguacaa cgugauuaga aagaaaggca agucccucug ggggauaagg      120

cuuuucgaug gagcaaauac ugcccaagaa cggcgcacgu uucgagcgcu gcguagaagg      180

aacgagcgga aaaagcagcg caucaauuug uuacaggaac uguucugcaa agagauuugu      240

aagcuagacc ccaacuuuuu caucagacuc agggauucuu cacuguggag ugaagacaag      300

gaggagaaac agauuuauag ccuuuuuaau gauaagaacu ucaaugaccg gaacuaccac      360

aauaacuaua aaaccaucua ccaucugcgc gcugauuuga uaaagaauaa agaaaagcac      420

gacgcccgac ucguauaucu ggcaauccau cacauuauga agaacagagg ccauuuucug      480

uucaauggua acaucgaguc cgugacaucu uuugaaaaca cuuucaauga guuuaaaaac      540

ugccucuuag augagcugga aauuaaucuu gaguguccuu cgaucucaga guucgagaac      600

auauugaagg acaggaccuu uaaagggaca caaaagugca aaaagcugaa agaacuaugu      660

agcauuccaa guuccgauaa gaagcucgac aauaucuuca aacugauuuc uggacuuaag      720

gccgaccuga gcaucaucuu cgaggaugag uccuuuaaag aaaacgagca caauaagauu      780

ucauucgcug acaguaacua cgaagagacc cgggaagucu ugaauagcga gauacaggag      840

aaaacucuca ucauugauau cuuuaacucu auauacaauu gggugauucu guccgacauc      900

uuaucaggcg gcgaauauaa cgggggacug uaccucagca ucgcaaaggu uaauacauau      960

aacaagcaca guaaagaucu uaagauucug aaaucuguga ucaagaaaua cuucccgcag     1020

aaguauaccg acauuuuugc cuccaauaag gagaacaacu acugcagcua uauagguaug     1080

uacaaaucgg gcgggaagaa auacucuguc aagaaauguu ccgauaagcc cgaagaguuc     1140

uauaagacga ucaaaaauau uaucaacaag aauacugcug aagagaucuc aaacgacaaa     1200

gaugugcagu acauuuugag ugagauagaa aauaacagcu uucuaccucu gcaaaucaau     1260

aagaacaaug gaguaauucc auaucaggcc aacaaaaucg agcucgaauc cauucugaag     1320

aaugcggaga acuaccuucc cuuccugaaa gagaucgaca ccgaaugcaa uaagacaacu     1380

ucugagaaga uaaucgauuu guuugaguuc cguauuccuu auuacguugg cccacucaac     1440

accagcaaag gugaaaaugc auggaugauc cgcaaggaag acgggacaau uagacccugg     1500

aacuuugagc agaaagugga caucgauaag accgccgcug aguucauaac gaggaugacu     1560

aaccagugua cauaucugaa uaacgaaacc gucuuaccua aauacucacu gcuauacagu     1620

gaguauacug ugcucaauga aauuaacaau auccggauua acggagagaa gaucuccgug     1680

gaacugaagc aagacauauu uaauauccuu uucaaaaaca auaagcaggu cacaugcaaa     1740

aaguugcuga gccaucucaa cucuaauggc uacaaccuga aaaaggauga ccuuucaggc     1800

uucgauggga auuuuaagag uagccugucc ucuuauauug acuugaaaaa cuacaucuuc     1860

ggagauaaua uugacaagcc acagaucuau aaccucgccg agaaaauaau ucuguggauc     1920

accuuauacg gugaugacuc gaagcugcua aaacgaguuc uccaaaagaa cuguagcgag     1980

cagcugaauc cggaacagau caagaaaauu acgaaacuua aauuuaaggg cugggggcgc     2040

uuguccaaag cauuccuguc agagcucaga ggagcugaua gggaaaccgg cgagacaaug     2100

acuaucauua agggucugcg gaacacccag aauaacuuaa ugcaacugcu uagucagaau     2160

uauacauuua cugaagagug cgagaaaaua aacucugggu acuucaucaa ucccaacgaa     2220

auuagcuacg acuccaucgu gaagaauauu guagccucac cuagcaucaa gcgcucugug     2280

uggcagacca uacagaucgu cgaggaaauu caaaaaguga ugggcuccaa gccaagaaaa     2340

aucuuuguug agauggcgag gggagaggaa gagaaggugc guacaaccag ucggaaacag     2400

aaguugcucg aucuguauaa gaacauuagc gacgaaugcg ccaaaggcau guacgacgag     2460

aucuauucua aggaugaauc cgacuucaaa aauauacgac uauaccugua uuacacucag     2520

augggguguu gcauguauuc aggaaagcac auugaucuca gucagcugag caacacgaau     2580

aucugggaca gagaucauau cuacccccaa ucgaaaacaa aggacgauuc ccuugacaac     2640

uugguccugg uguacaaguc uauuaauagc gagaaaucag augacauaau caguccuacc     2700

auucagaaca auaugaggac uuuuuggaag uuacucuucg aucgcaaauu uaucuccgag     2760

aagaaauauc accggcugac cagaacaacc ccacuuacug acgaagaacu ggcagguuuc     2820

auuaacaggc aguugguaga aacacgccag ucuacgaagg uuauggcuag caucuuuaag     2880

cgaauauacc ccaacaccga gaucauuuau gugaaagcca agaaugucuc cgauuuccgg     2940

caccguaaag acugugaaaa cggcgugaag gucagagagc ucaaugacua ccaucacgcu     3000

aaagaugccu aucugaacau cgugguuggg aauguguacu caagcaaguu cacuaacaau     3060

ccgcuaaugu ggcugaagaa caauaggaac guaaaauaua gucucaaucg cauguacgac     3120

uuuaaccuga cagucaauaa cgagaauauu ugggugguug gaaacgaagg cacccaaaag     3180

acagugaaca aauaccuugg uaagaaugau aucuuguaua cucgguacgc aaugaccaac     3240

aaaggggagc uguucgacca gaauccuuua aaggccacgg gcgauaaauc uaagcagacc     3300

acacucgucc cccugaagaa aggaaaggaa guguggaaau auggcgggua caacuccaua     3360

acuccaagcu auuuuaugcu uguagagucg aaggacaaaa agggaaauua cauuagaacc     3420

aucgaaacaa ugccucugua uagggagaag gaguucucaa agaacgaaga guuucuacuc     3480

aauuacugca aggaagugca uaaacugaag gagcccaaaa ucauuuugaa guguaucaag     3540

aaaaacgcuc uguuaauagu ugaugguuuc ccaaugcacc ucaagggcuc uacugggcug     3600

caacuuaaac ugcagggagc gguccaguug auucucgaga gugacauggu gaucuaccug     3660

aagaaacuaa ugaaguuugu gaaugaaaac aauauuaaca agaauaaacu ggaggucacc     3720

cccuauaugg aacuuuccgc aaaggagaac aucaaacucu acgauauauu cauucagaag     3780

cugacgacag gcaucuauag caaacgaccu gccaauccag uugaaaaguu gaucaacgcu     3840

cgcgacauuu uuaucgagcu gaccgugaaa gagcaaugca uuguguuauc ugaaauacuc     3900

caucuguucc aguguaagcc gcuuacuagc gccaaccugg uauccaucaa uuugaagaaa     3960

acagucggua uuauccagau cgcaaagaac auuaccgcca aaaaugugaa gcucguucac     4020

cagucaccca cugggauaua cgaguauacc guggaucuga acaaaaucug a              4071


<210>  3048
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3048
augaccaaga aagagcagcc cuacaacauc ggccuggaca uugggacaag cuccguggga       60

ugggccguca cuaaugauaa cuaugaccuc cuuaauauaa agaaaaagaa ccuguggggu      120

guuagauugu ucgaagaggc ucaaacggca aaagaaacca gguuaaaucg gucuacacgc      180

cgacguuaca gaaggcggaa gaaccgcauc aauuggcuga acgagaucuu uucagaggaa      240

cuagccaaga cugauccuag uuuccucauu agacugcaga auagcugggu guccaaaaag      300

gacccagaua ggaaacggga caaguauaac cuuuuuauug auggcccgua caccgacaaa      360

gaguauuacc gcgaguuccc cacaaucuuu caucugcgaa aggaauugau acucaauaag      420

gauaaagcgg acaucagacu gauuuaucug gcucuccaca acaucuuaaa guacaggggg      480

aauuucaccu acgaacacca gaaauuuaac auuucuaauc ugaacaacaa ucuuucgaag      540

gaguugaucg agcugaacca gcaacuaaua aaauaugaua uuucauuccc ugacgauugc      600

gacuggaauc auaucagcga cauucucauc ggacggggca acgccacuca gaagaguucc      660

aauauccuga aagauuuuac acuugacaag gaaaccaaga aacuguugaa ggagguaauu      720

aaccucauac uggguaaugu ggcacacuua aacacgaucu ucaaaacuuc ucugaccaag      780

gaugaagaga aacucaauuu uagcgggaag gacauugaau ccaaacuuga ugaccuggau      840

ucaaucuugg acgaugacca guucacaguc cuagaugccg cuaaccguau auacaguacu      900

auuacccuga augagauccu caacggagag agcuauuuuu cuauggcaaa agugaaucag      960

uacgaaaacc augccaucga ccuguguaaa cuucgcgaca uguggcacac aaccaagaau     1020

gaggaagcug uugagcaauc cagacaggcc uaugaugacu acauuaacaa gccaaaauau     1080

ggcacgaagg aacuguacac uucauugaag aaauuccuca agguggcgcu gcccacaaac     1140

uuagcaaaag aggccgagga aaagaucagc aaaggcaccu accuggucaa gccuaggaau     1200

agugagaacg gggugguacc auaucagcua aauaagauug aaauggagaa aauaaucgau     1260

aaccagucuc aauacuaucc cuuccucaag gaaaauaaag agaagcugcu uuccauuuug     1320

agcuuucgga ucccuuacua uguuggacca cugcagucgg cugagaaaaa cccguucgcc     1380

uggauggaac gaaagucuaa uggucacgca cgccccugga acuuugacga gaucguggau     1440

agagaaaaau ccucaaauaa guucauuagg cggaugacug ucacagacag uuaccucgug     1500

ggcgagccug ugcugccaaa gaacagccuu auauaucagc gcuacgaggu ccugaaugaa     1560

uugaacaaua ucagaauuac cgagaaccuc aaaacgaauc ccaucggguc caggcugacc     1620

guugaaacaa agcaacguau uuacaacgag uuauuuaaaa aguauaaaaa ggugacugua     1680

aagaaacuga ccaaguggcu aaucgcucag ggauacuaua aaaacccuau acucaucggc     1740

cugucucaga aggaugaguu caauagcaca cuuacuaccu acuuggacau gaaaaagauu     1800

uuugguucaa guuucaugga agauaacaag aauuaugacc agaucgagga acugauugag     1860

uggcucacaa ucuuugaaga uaaacaaaua cugaacgaga aguuacauuc cagcaaauac     1920

ucuuauaccc cagaccagau uaagaaaauc ucaaauaugc gguacaaggg guggggacga     1980

cugaguaaga aaauucuuau ggauaucacu acggaaacaa acacucccca guugcuccag     2040

cugagcaauu acuccauacu agaccugaug ugggccacua acaauaacuu caucucuauu     2100

augucgaaug acaaguauga uuuuaaaaac uacaucgaga aucacaaccu caauaagaac     2160

gaggaccaaa acauuagcga ucuggugaau gacauccaug ucuccccggc gcuuaaaaga     2220

ggcauaaccc agucaauuaa gaucgugcag gaaaucguua aauucauggg ccacgccccu     2280

aagcacauuu uuaucgaggu gacaagggaa accaagaaaa gugagauuac uacaucucgc     2340

gaaaagcgga uaaaaagauu gcagagcaag cuguuaaaca aagcaaauga uuucaagccc     2400

caacucaggg aguaucuggu cccaaacaag aaaauccagg aggaacuuaa gaaacauaag     2460

aaugaccugu ccucagagcg cauuauguug uacuuccucc agaacgggaa aagccuguau     2520

ucugaagagu cccuaaauau caacaagcug agugauuacc agguggacca cauucucccu     2580

cgaacguaua uccccgauga cagccuggag aauaaggcuc uuguauuggc caaagaaaac     2640

caacggaagg cugaugaccu guuacucaau ucuaacguua uagaucguaa ucuggagaga     2700

uggaccuaca ugcuuaacaa uaacaugauc ggacugaaaa aguuuaaaaa ucuaacuagg     2760

cgcgugauua cagacaagga caagcucggu uucauccauc ggcagcuggu ccagaccucc     2820

caaaugguga aaggcgucgc caacauuuug gauaacaugu acaagaauca ggggacaacu     2880

ugcauccagg caagagccaa ccugucaacc gcuuuuagga aagcguuaag uggacaggac     2940

gauacguauc acuucaagca uccagaacuc gugaaaaauc gaaacguuaa ugacuuucac     3000

cacgcacaag augccuaccu ggcuagcuuc cuuggcaccu aucgccugcg gagauuuccc     3060

acaaacgaga uguugcucau gaauggugaa uacaacaagu ucuauggcca ggugaaggag     3120

cuguacucga aaaagaaaaa gcuaccugac uccaggaaaa auggguuuau aauuucucca     3180

cugguaaacg gaacuaccca guaugaucgu aauacaggcg agaucaucug gaacgucggg     3240

uuccgcgaca agauucuuaa aauauuuaau uaccaucagu guaacgugac ucggaagacc     3300

gaaaucaaga cgggacaauu cuacgaucag acaauuuaua gcccgaaaaa ucccaaguac     3360

aaaaagcuca ucgcccagaa aaaggacaug gauccuaaca uuuauggugg cuuuucaggg     3420

gacaacaaga guuccaucac cauaguuaaa aucgacaaua acaagauuaa accaguggca     3480

auccccauua gacugaucaa ugauuugaag gacaaaaaga cucugcagaa cugguuagag     3540

gaaaauguca agcacaaaaa gucuauacaa auuaucaaaa acaaugugcc uaucggacag     3600

auuaucuaca gcaagaaagu aggccuccug ucccuuaacu cagauaggga gguggccaau     3660

cgacagcaac ugauauugcc acccgaacau agcgcucucc ugcggcuacu gcagauuccg     3720

gacgaggauc uugaccagau ccucgccuuc uaugauaaga acauucuggu ugagaucuug     3780

caggaauuaa uaacaaagau gaaaaaguuc uacccuuuuu auaaagguga gcgcgaguuc     3840

cugauugcaa auaucgaaaa cuuuaaucaa gcgaccacca gugaaaaggu caacucucuc     3900

gaggagcuga ucacccuucu gcacgcuaau uccacaagcg cccacuugau uuucaacaac     3960

aucgaaaaga aggcauuugg gagaaaaacg cauggccuaa cccugaauaa cacugacuuc     4020

auuuaccagu cggugacagg acucuacgag accaggauac acaucgaaug a              4071


<210>  3049
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3049
augaccaaga aagagcagcc cuacaacauc ggccuggaca uugggacagg aagcgugggu       60

ugggccguca cuaaugauaa cuaugaccuc cuuaauauaa agaaaaagaa ccuguggggc      120

guuagauugu ucgaaggggc ucaaacggca aaagagacca gguuaaaucg guccacacgc      180

cgacguuaca gaaggcggaa gaaccgcauc aauuggcuga acgaaaucuu uucugaggaa      240

cuagccaaua cugauccuuc auuccucauu agacugcaga acaguugggu gagcaagaaa      300

gacccagaua ggaagcggga caaauauaau cuuuuuauug auaacccgua caccgacaag      360

gaguauuacc gcgaguuccc cacaaucuuu caucugcgaa aagaauugau aaucaauaag      420

aacaaggcgg auauuagacu cguauaucug gcucugcaca auauccucaa auacagggga      480

aacuucaccu acgagcacca gaaguuuaac auuuccacuu uaaauucuaa ccugucgaaa      540

gaacuuaucg aguugaauca gcaacugaua aaguaugaca uuucauuccc ugauaacugc      600

gacuggaauc auaucagcga uauucuaauc ggccggggua acgccacaca gaaaaguucc      660

aauauccuca acaauuuuac ccuggacaag gagacgaaaa agcugcuuaa ggaagugauu      720

aacuugauac ucgggaaugu cgcacaccug aacacuaucu ucaaaaccuc uuuaacaaag      780

gacgaggaaa aacugagcuu uuccggaaag gauauugagu caaaacucga cgaucuugac      840

aguauccugg augacgauca guucacugug uuggacaccg ccaaucguau auacagcaca      900

auuacccuaa acgaaauccu gaauggcgag ucuuauuuuu ccauggcuaa gguuaaccag      960

uacgagaacc augcaaucga ucucuguaag cugcgcgaca uguggcacac gacuaaaaau     1020

gaaaaggccg ugggccuuuc aagacaggcu uaugaugacu acauuaacaa accaaaguau     1080

gggacaaaag agcuguacac cagcuugaag aaguuccuca aagucgcccu gcccacuaau     1140

uuagcgaagg aagcagagga gaaaaucagu aagggaacau accuggugaa accuaggaac     1200

ucugagaaug guguaguucc auaucaacua aacaagauug agauggaaaa gauaaucgac     1260

aaucaguccc aguacuaucc cuuccucaaa gagaacaagg aaaaacugcu uagcauuuug     1320

ucguuucgga ucccuuacua ugugggccca cugcaaucuu ccgagaagaa uccguucgcc     1380

uggauggaac gaaaaucaaa cgggcacgcu cgccccugga auuuugauga gaucgucgac     1440

agagagaaga guagcaacaa auucauuagg cggaugaccg ugacggauuc cuaccucgug     1500

ggagaaccug uccugccaaa gaacucucuu auauaucagc gcuacgaggu ucugaaugaa     1560

uugaauaaca ucagaauuac cgagaaccuc aagacaaauc ccacuggcag caggcugacc     1620

guggagacaa aacagcauau cuacaacgaa uuauuuaaga auuauaaaaa gauuacugua     1680

aagaagcuga ccaaguggcu aaucgcccaa gguuacuaua aaaacccuau acucaucggg     1740

cugucacaga aggacgaguu caauaguaca cuuaccacuu acuuggauau gaaaaagauu     1800

uuuggaucca gcuucaugga aaacaauaaa aacuauaauc agaucgagga acugauugag     1860

uggcucacga ucuuugagga caagcagaua cugaacgaaa aguuacacuc uucaaauuac     1920

aguuauacaa gcgaucaaau uaaaaagauc uccaacaugc guuacaaagg cuggggccgg     1980

cugucuaaga aaauucuuac cugcaucacu accgagacaa auaccccaaa gucguugcag     2040

cucagcaacu acuccgugcu ggaccuaaug uggacuacaa auaacaacuu cauaucaauc     2100

auuaguaaug auaaguauga cuuuaaaaac uacaucgaaa aucauaaccu gaauaagaac     2160

gaggaucaga auauuucuaa ccucgucaau gacauccacg ugagccccgc acugaaacga     2220

gggauaacgc aguccauuaa gaucguucaa gaaaucguga aauucauggg acacgcuccg     2280

aaguauauuu uuaucgaggu caccagagag acuaaaaagu cagaaauuac aaccagcagg     2340

gguaagcgca uacagcggcu ucagucuaaa uugcugaaca aggccaaugg cuucaaaccu     2400

caguuaagaa aguaccucgu gcccaacgag aaaauccaag aagaacugaa gaagcauaaa     2460

aaugaucuuu ccagugaaag gauuaugcug uauuucuugc agaacgggaa aagccucuac     2520

ucugaggagu cccugaauau caacaaacua ucagacuauc agguagacca cauucugcca     2580

cgcacauaca ucccugauga cagucucgaa aauaaggcgc ugguucuugc caaagagaac     2640

cagcgaaagg cagaugacuu gcuguuaaac agcaauguga uagauaagaa ccucgaacgg     2700

uggacuuaca ugcugaauaa caauaugaug ggacuuaaaa aguuuaaaaa ccugacccgu     2760

agagucauca cggacaagga uaaacuaggc uucauucaua ggcaacucgu gcagaccucg     2820

cagaugguca aggguguggc uaauauccug aacuccaugu auaagaauca agggacaacu     2880

uguauucagg cccgcgcuaa cuugucuacc gccuuucgga aagcacugag cgaccaggau     2940

gacagauacc acuucaagca ucccgaguua guuaaaaaua ggaacaucaa ugauuuucac     3000

cacgcccagg acgcuuaucu cgcgucauuc cugggcacau accgacuucg ccgguuucca     3060

acugaugaga ugcuguugau gaacggagaa uauaauaagu ucuacggcca agugaaagag     3120

cucuauagua agaagaaaaa gcugcccgac uccaggaaaa acggguuuau aauuucuccu     3180

cuaguaaacg gaaccacgca guacgacaga aauacaggug aaaucaucug gaacgucggc     3240

uuccgugaua agauucugaa aauauuuaau uaccaucagu gcaacgugac ccgcaagacu     3300

gagaucaaaa cagggcaguu cuaugaccaa accauuuaca gcccaaagaa uccgaaguau     3360

aaaaagcuca ucgcacagaa aaaggauaug gaccccaaca uuuacggagg cuuuuccggu     3420

gauaauaaau caagcaucac uauaguuaag aucgacaaca auaagauuaa accuguggcc     3480

aucccaauuc ggcuuaucaa cgaucugaag gacaaaaaga ccuugcagaa uuggcuggaa     3540

gagaacguca aacacaagaa gaguauacag auuaucaaaa auaacgugcc caucgggcaa     3600

auuaucuauu cuaagaaagu aggcuuacuc ucccugaaua gcgauagaga gauagcuaac     3660

aggcagcagc uuauucugcc uccagaacau ucggccuugc uccgaauccu gcaaauuccc     3720

gacgaggauc cggaccagau ccuagcauuc uacgacaaga acauacuggu ggaaauucuu     3780

caggagcuca ucacaaaaau gaagaaguuc uauccuuuuu acaaaaauga acaggaguuc     3840

cuggccucaa acaucgagaa uuuuaaccaa gcuacgaccu cugaaaagau uaauaguuug     3900

gaggaauuaa ucacucugcu ccacgccaac uccacaagcg cacaccugau uuucaauaac     3960

auagagaaaa aggcguuugg acggaaaacc cauggccuua cacugaauga uacugacuuc     4020

aucuaccagu cuguuaccgg guuguaugaa acgcgcauuc acaucgagug a              4071


<210>  3050
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3050
augagcaaga aagacuccga guacuauauc ggccuggaua uugggaccaa cucuguggga       60

ugggccguca caaaugguaa guacgaccuc cuuaacauaa agaagaaaaa ucuguggggc      120

guuagauugu ucgaagcugc agagucagcc gcggggaggc ggaucaaccg caguacucga      180

cguagauaua ggcggcgcag aaauaggauu aacugguuaa augaaaucuu ugcugaugag      240

cugagcaagg uggaccccuc cuuccuaacg cggcucaacu cuucguggau uucaaagaaa      300

gauagcaguc gcacccgaga caaguacaau cuguuuaugg augccgacua uacagauaaa      360

aacuaccacc auaaguaucc uacuaucuac caccuuagaa gggaccugau guucgaugac      420

aaacaguuug auauacgguu gguauaccuc gcacugcaua auaucauuaa guaucgugga      480

aacuucaccu acgaccaccg caaguuuaau guguccgaaa ugucuagcaa ccugacaaaa      540

gcccucgaga auuucaacca agaguuaaug gaauuugagg acgcuuuccc agauaacacc      600

gacuauuccg caaucucaga aguccugcuu aagaguaaua acgugagcac uaaaauugau      660

gagaucauau cuaauuugga gcuggacaag gauaccaaaa aguaccuaaa agaaguucuc      720

aagcugauuc uuggcaacuc cgccaaucug aacacaaucu uucauacguc auuggacaag      780

gaugacgaga aacucagccu gagugcuaag gauauugaau uaaaacugaa uaaccucgac      840

ucucuucugu ccgaugagca gauccacuuc aucagcucgg ccaaugaauu guauucuucc      900

auuacucuaa acgacauacu ggacgguaag accuaccucu cacuggcgaa aaucaagcag      960

uaugauacac acugcaguga ccuucaggag cugaaggaga ugugguacaa aacuaccaau     1020

ccggauuuga uuaagaaaag cagaaaggca uacaaagaau augugcaugg guccuacaac     1080

acagaggacu uuuauaaggc caucucuccc uuccucaaga uagcuauucc ugaagaucug     1140

gccaaagacg cauuaaagaa aaucgaugcu aauaccuacc ugccaaagca aaggacgagc     1200

aacaauggag ucauccccuu ccagcuaaac cucauugagc uggagaaaau cauugacaac     1260

cagucaaagu auuacccuuu ucuuaaggaa aaucgggaga aaauaaucag uauuuugucc     1320

uuccgaaucc cauauuacgu gggccccauc caggccccua cugauaagaa cuuuagcugg     1380

augguuaaaa agauucaagc gaaagcccgc ccauggaauu ucgacgaagu aauagauaga     1440

gagaagucuu caaacaauuu uaucaaaagg augacaacca cugacacaua ccuggugggc     1500

gaaccggucc uccccaagaa cagucugauu uaucagaagu ucgaggugcu uaaugaacug     1560

aacaauaucc ggauuagcaa aaacuugucc caggauccuc ucgggucuaa gcugucggug     1620

gaggucaaac agcaaaucuu ugaggaauua uucaagcgcu acaaaaccgu uaagagcgac     1680

cagcugaaga aauggcuaau aagaaauucc uauuuuacgg acccaaccau cauuggacuc     1740

ucaaagccca gugaguucaa cucuagccug uccacauuua ucgauaugaa agcaauuuuc     1800

gguauggacu uugugaauaa cccuagaaau ucaagccagc uugaagaguu ggcugagugg     1860

cugacuguau ucgaagauaa gcaaauccuc ucugagaaaa uacgucacuc caacuaccca     1920

uauagugacc agcagauucg gaagaucagc aacaugcgau acagaggcug ggggaggauu     1980

ucuaagaaac uguuauguga uaucaaggug gaauccacca uauauaaauc acaucagcug     2040

caccccuaca guauccuuga cuugaugugg agcacaaaua acaauuucua cucgauucuc     2100

cacaaggaua aauauaacuu ugaguccauc cuggaagacu acaaucuagg aacugaugac     2160

aagaccgaua uuaacgagcu ggucgacaau auccauacau cuccggcccu caagcgcggc     2220

auaauucaaa gcaucaacau cgugaaagag cugguuaagu ucauggguca cgcuccucag     2280

cauauuuuuc uugaguucac ccgggaagau aaaacuucaa cguugaguga auccagaaag     2340

aaaaggcuga agaaauuaua uaauaaccuc ucugaggaca caaagaaguu uaaacuggac     2400

cuucgcgauu accuggugcc cgacaagagc auccagaaag aauuggaguc ccacgauaag     2460

gaccucucaa aagagcgacu gaugcuauau uuccugcaga augggaaaag ccucuacagu     2520

gaugaagccc uggacauuaa ccggcuuucu gaguaucaag ucgaucauau auugccacgu     2580

accuacauca aggacgauuc ccuggaaaau aaagcauuag ugcucgccaa ggagaaccag     2640

agaaaagcug acgaucugcu ucugcagccu agcauuauca auaagaacau ggaaaggugg     2700

auuuacauga aagaccacgg aaugaucucg cccaagaagu uuaaaaaccu aacucgcacc     2760

acaauaaccg aggacgauaa gguacacuuc aucaaucggc agcucguuca aacuucacag     2820

auuaucaaaa acguggcgaa uauucuggac aacauguaua aggagcaggg cacaacgugc     2880

gucgaaacca aagcaaauuu gucuagugcc uuuagaacug cuuucuccga gcaggaugac     2940

aaguaccauu uuaagcaccc agaguucauc aaaaacaggg augugaauga cuuucaucac     3000

gcccaagaug cauaucuggc cuguuuacuc ggccuguacc gaauacagcg cuuccccagc     3060

gacgaaaugc uucugauuaa gaaagaguau cggaaguucu uugcucaggc ccaaaacucu     3120

gucaaaaauc acgggaagau cccugauagc cagaagaacg gauucaucuu ggcaccacuc     3180

gugaauggug aaacacugau uaacaauaaa accauggaga caauauggaa cauggacuac     3240

aagagaaaag uuaucaagau uuuuaauuau aaacaguaca acaucacuaa gaggaccgaa     3300

auuaaaacgg gcgaguucua caagcagacc cuauauuccc cgcaugauga aaaugugaag     3360

aaccccaucc cucagaaaaa gaaccuggac ccaaaaauau acgggggaua uucaggcgua     3420

caaaguagcu accuugcgau cauuaagguc gaucagaaaa agcccacaau ggugucuguu     3480

ccuauccguu uuuccaagau gauugacgac gguaaacucu cacugcagag uugguuggag     3540

aauaacauca agcacaaaaa gacuauagag gugcugaaaa ccaagauucc aaucgggcaa     3600

uuaacauaua aucccaagaa cggcuaccuc agcguccagu ccgaaaaaga gaucgcuaau     3660

gcccagcagc uguuccuucc guuugaacau guggcacugu ugacucuccu gcacgccccu     3720

accucugagu augaucaaau ucuaucguuc uacgacacgg auauccugcu ugaaauauuu     3780

gacgagauua caaacaagau caaaaccuuc uuuccauucu acauugauga gcaggaauau     3840

cucguaagca uccgcgagaa uuuccaguca gcuacuguga uagaaaaggu uaacguccug     3900

caacaguugu uaauuaugcu gcaugccaau uccagucgga ucucuaucaa acucggagag     3960

gugagcaaga aggaccuggg caggaaaaag aaaaggauuc cccuugaaaa cacagaucug     4020

guguaccagu ccgucaccgg guuguaucag acuaaguuuc acaucgacug a              4071


<210>  3051
<211>  4074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3051
augaacgaca gaaauaagaa auacaacauc ggccuggaua uugggaccgg aagcgugggu       60

ugggccguca cagacaauaa cuauaaucuc cuucacgcua agaaaaagaa ccuguggggc      120

auaagguugu ucgauggggc agagacugcc gcgucccggc gcacguuucg aucuacccgu      180

agaagguacc ggcgcagaag gaaucggauc aacugguuaa augaaauuuu cgcugacgag      240

cuggccaaaa aggaucaguc auuucuaguu cgcaugaaca guagcugggu guccaagaaa      300

gacaagaucc gacaaagaga ucaguauaau cucuucaugg acucugauua cacagacgua      360

gaauaucaua gggaguuucc cacuauuuuc caucugcgga aacgucuuau cgaagauaag      420

ucgcaggcag acauacgccu gguguacuug gcccuccaca acaucauuaa auauagagga      480

aauuuuaccu acgagcacca gaacuucgau gucucacaua uggacagcgg ccuggcucug      540

cucuuaaauc aacugaacca gcagcuucag gcauuugagg uggccuuccc ugacgaaaca      600

aacuuugaga guauguccac caucuugcug gaaaagaaga cuccaacaca gaaaauugau      660

gagaucguug accaaauaca gccggagaag gaucuacucc agauuaccaa aaauaucaug      720

aagaugcugc uugguaacaa agcugaccug gugaagauuu ucucuuugga uagcgacgau      780

aaaaucacgg ucgaauuuuc cucaaauagu aucgagcaag accucagcaa ccuggaagag      840

acuuuaucug augaacaguu caauauuaua acccuggcca acuccaucua cucaagcauu      900

gugcucaaug acauccuuag uggggagaca uaucugucuu ucgcgaaggc aaggcaguac      960

uccgaucaca agaaagacuu ggccaagcua aaaacuaugu ggcgggagag ccccgaccga     1020

aaggcuguag gaaaagccaa gaaggcauau gaugacuacc ugcacucggg caaauauggc     1080

auagaagauu uuuacaagga caugucuaaa uuccucgaga ccgcuacacc uguugaacug     1140

gccaaggagg cgaacgagaa aauucuuaau aaguccuauc uguugaagca acgcaacaau     1200

gaaaacgggg ugaucccauu ucagcucaau aaaaacgaga ugaucgaaau uaucaauaac     1260

caggccaagu acuaccccuu ccuggcagag aacaaagaua aguuacuguc aauucuaagu     1320

uuuagaauac cuuauuacgu cggaccacuc cagagcaaaa aggacaaucc cuucgcuugg     1380

auggaaaaga aaucccaagg uaagaucagg ccuuggaacu uugaugagau uguggaccgg     1440

gaagccucua gcaauaacuu cauccgcaga augaccucaa cggauacuua ucugaucggc     1500

gagccagugc uuccguugaa aagucccauu uaccagaagu augagguccu gaaugaacuc     1560

uccaacguua ggauaaaugc ugacgaggug gauaacccuc ugggggacaa acuuagcgua     1620

gaucugaagc aggccaucua caaugaauug uuucucaaac aucguucugu gucaaaggca     1680

cuguuaacaa aguggcugau uaaccagagu uauuuccaaa auccacagau cagcggacua     1740

gccgacucca aaaaguuuaa cucuggccuc accacuuacc acgacuucaa aaagauuuuu     1800

ggugaggauu ucgucaauga ccccacaaac cagucgcagc uggagcaacu ugcugaaugg     1860

uugaccaucu ucgaggauaa aaagauacug aagaucaaac ucgacgauag ccaguuuaau     1920

ugcacgccug aacagauuaa gaaaaucgcg gggaugcggu accagggaug gggccgacug     1980

uccaagaaau uacuguguga cauuaagacc acaacuguga ccucaaagaa ccaucaacag     2040

gcaaguuauu cuauccuuga uuugaugugg aacacaaaua gaaacuucau aagcguucuc     2100

aaaaaugaca cuuacaaguu ugaggauaaa auucagaccu ucaaccugga caagaaugaa     2160

gccgcuacac cacaagccau gaucgaugag cuacagaccu cccccgcacu gaaaaggggc     2220

auucgccagu cagugacuau cauacaggag cucgucaagu uuauggggca cgccccggaa     2280

cacaucuucc uggaguuuac gcgggaagac gaugugagcu cucuuacaaa guccagagac     2340

aguagguuga acaaacugua ugcucgcauu gccaagaaua ccaaaaagac ugacgaugag     2400

cucaaaaaga gcuuacugcc uucuuccgac aucaaagcaa cccuuaaggc gaacaagaau     2460

aaccugucaa augaucgauu gaugcucuac uaucugcaaa ugggaaaaag ucuauacagc     2520

aaccagcccc uggacauuaa ucggcucucg gaauaucaga ucgaucauau acugccacag     2580

uccuacauua aggacaacuc ucuugagaau cgugcuuugg uacuggccag cgagaaccaa     2640

cacaaaucaa gugauuuacu ccugaagccu gaaaucauca accagaauau ugcaagaugg     2700

agguacauga aagacgccgg ucuuaugggc cccaagaagu ucaaaaaccu gacacgcauc     2760

cagauuaccg auuccgacca gaauaacuuu auaaaucggc agcuaguuca aacuucucag     2820

aucauuaagc augugagcaa cauccucgau uccauguaug gggaccaagg aacacagugc     2880

gucgagacga gagcucaccu gucaaccgag uucaggaaag ccuuuagcaa ugaagacgau     2940

aacuaccauu uccagcaccc agaauuugug aagaaucgaa acauuaauga cuaucaccau     3000

gcacaggaug cuuacuuggc cugucuguua ggccucuauc aaaugaaaaa guaccccacu     3060

gacaacauga uccugcuuaa gaaagaguau aagcgcuucu ucaguaauac caaaaagaua     3120

uacgauaaaa agcaccauuu uccugacuac gucaagaacg guuggaucau ugggucuaug     3180

uucaauggcg agaaauaugu ggaugaagag acaggacaga ucauuuggga ccagcagaca     3240

aaggauaugg uuuccugcau cuuucaauac aaacaguaua acgugacuaa gcggaccgaa     3300

gcggccaaug gcgaguucua caaccagacg guauauaaaa aggacccaaa cgagaagaga     3360

aaacugauau cauugaagca ggggcucgau accaaacugu acggagguua uucgggcgac     3420

aauccggcuu acauuguccu agugaagauc gaugacaaaa agaacaaacu gguuaagaug     3480

cccaucaggc uugcagaaca aguggacaau aagacaauug aucuccagac uuggcuggag     3540

gaaaacgucc cucacaaaaa ggggauacag guguugaaaa ccaagauccc acugggacag     3600

guaauuuaca gcaaaaagaa uggcuauuua gcccucaagu cugacacaga gaucgugaac     3660

gcucaacagc ugguucuucc cuacgguuau gucgcccugu ugacucuccu gcagaaaaug     3720

ccugaaaaua aguacgauga ggugcuagca uguuaugacg augggauucu ggacaacauc     3780

uuuguggcga uaaucgauaa aaugaagcac uucuacccau auuacagugg cgagcuuaaa     3840

acccucuccg acaauauuga uaacuuuaag gaaagccccg acgucaauaa gaucaaaacg     3900

cugcaacagu uguuacugcu ccugcaugcc ucuagcacaa ccgcuuccau ugaguucaac     3960

aauguuaaga aaaacucacu uggacguaag ccgaauagua ucaacauaca gaacacugau     4020

cuguugagcu uuucugccac aggccuauac gaaucccgca uucacaucga cuga           4074


<210>  3052
<211>  4074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3052
auggccgcua agaacuacaa uaucggccug gacauuggga ccagcuccau aggaugggca       60

aucguggaug acaacuauca gcucaugcac gccaaaggua aguacggcua ugggacaaga      120

auuuacucug agggacaaac ugcggcugau aggcggggcu uccgcacgac ccgacguaga      180

cuuaauaggc ggaaauggcg ccugagauug uuacaggacu uuuucgaacc cuauauccug      240

ccucaggaug aggccuuuuu cauuaggcag aagcaaucaa accuaguccc acaggacacu      300

aaaaagcagu acccggaaag uguucucuuu gaugacacua gcgauaagga cuucuauacc      360

gaguacccca caaucuacca ucugcggcag gcacuuauga ccgaaaaucg ccaauuugau      420

auacgagagg uguaucuggc cauccaccau auuguaaaau acagagguca cuucuugaac      480

aaugcuccug ugaagacuuu uaaauccggg aaccucgacc uggcaaucaa guucaaugag      540

cugaacgaag aguuuaaauc ugucuaucca gaucucgaau uacaguucaa ucuggaccag      600

auugacgaga ucaagaagau acuuuuggau ucgacaaaau caagguuuga ccggcaggga      660

gaggccguga agcugauuua caccguuagc gaagugaaaa cgaucgagaa gcuacaaaac      720

aaaauuguca agcaguuccu caguggcauc cugggccuua auacucguuu cgaugugcug      780

accaacacag aauccgacga uucuaugugg aaauuuagcu uggacuccga gucaagugau      840

gacgcucucg augaaaucau ugcccagcug gacgaucccg cgauacaugu aguugaguua      900

gugaaugaga uccacagcuc uauuacucug gcagggaucg ucccugaagg acaaggucuc      960

uccgagucaa ugguggaaaa guaugaggcc cacaaggcuc aucuucagcu guugaaagcc     1020

guggaaaagc uagucgacaa aaaggcagcu gacaaacugg agguugccua cgaucaguau     1080

auaaacggca aggacagcaa gcaguucauu aaagaggauu uuuacaaggc ggugcaaagu     1140

gaaaucaaag ggguagccgg agacgaggug gcagaaauca accagcucau ugagcuggau     1200

caguucaugc caaagcagcg caccaaagaa aauggcgcua ucccccacca acuucaucag     1260

cacgagcugg acgucauuau agagaaccag ucuaaguacu augauugguu gggugaaaag     1320

guggggaaug aguacaaacu cgacaagcug guuuccuuua gagugccuua uuacgucgga     1380

ccauuagugg auacagccag caaagaaaag aaagacgaga ccagguucgc uuggauggua     1440

cggaagaacc cggagucgac ggaagccauc acucccugga auuuugagga uauuguugac     1500

aagaacgcau cugccgaaaa uuucaucaaa cgaaugacaa ccaaggauaa auaucugcua     1560

aacgaggacg ugcucccuaa gugcucccug cuuacucagg acuuugaagu cuacaaugag     1620

uugaacaaua uccgcgugaa cggcgagaaa uucacagcug aacaaaagca gcugauucac     1680

gaugagcucu uuaccaaaca ugcgucaguc aguauaaagc agcugaagga cuuccuugug     1740

agcgaacuga uguauguuag agauaauuug aaaguggagg gccucgcaga cgaaaagaaa     1800

cugauguccu cuuuaagcac gcaguuugau uaccaaaaga ucauuccaga ccagaucaac     1860

gauguaaaau acagggagga cauugagcag aucauagaau ggucaaccau cuucgaggau     1920

gccgcuauuu ucaagacaaa gcugaaagaa aucgacuggc uaacugauga gcaacggaau     1980

aagcucgcca acaaacgcua uagagggugg ggaagucugu ccaagaggcu uuuggcaugu     2040

cuggucgacg aaaacgguca gcguauuauc gauauacucc gggacagcuc ugacaauuuu     2100

augaugauug ugaaccgacc cgaguucaaa caggccaucu uugaugagaa ugcuaaccug     2160

accgaaacaa auucaaagaa ggacauguuc gagaugauug auaacuuuua cacaaguccu     2220

cagaauaaaa aggccaucag acaaguuuua cugguggucc aggacauaca gaacgcaaug     2280

ggcggggugg cgccacagaa aaucuucauu gaauuugcua gggaggauga gguaaaugga     2340

cgccggacca acagcagaua uucccaaauc agggaaaugu acucuaaggu gacagacgau     2400

guuauuaaua aaaccguccg cggcgagcuu gaagccaaca aggguucguu gaauaaggag     2460

aaacucuucc uguauuuucu acaagggggc aucgaccugu acagcggaaa gacuauaaac     2520

auugauaaua ucuccucaua ugacguggau cacgugcucc cccagaguuu caucaaagac     2580

gacucuauua acaacaaggu ccugguuaau gcaaaaggca accgagugaa gaaugauaac     2640

gugccgaugg aacuuuuugg ggccaaaaug uacccuacgu ggaagcggau gguagacaca     2700

ggaaucauga gcaaggcuaa auucaagaau uugacccuga accccgauaa ugucgacaaa     2760

uuuaaggccg aggguuucau uaaccgucag uuaguggaga cuagacagau aaucaaacuc     2820

guugcacaga uucugaauga uaaacuuggc gacgauacca agaucguguc cgucaaagcu     2880

aaccugacac augacaugag gguguucuuu gauuucccaa agaaucgcaa cguuaauaac     2940

uaccaccaug ccuuugacgc guauuuguca gccuucgugg ggaccuaccu cuauaaacgg     3000

uacccuaagc ugcagcccua uuuuguauac ggagaguuca aaaagaugga uaagcuaaag     3060

aauaugacua gcuuugacuu ccugagagau cucaagcaca cagacaaagu cguggacaag     3120

gauacgggcg agauuaucgc ugacaaacug gcagugauaa ccaacaugaa caagaucuau     3180

gauuuuaaga aaguccuugu uacucacgaa gugcaugaaa auucuggugc cauguacgac     3240

caaacauugu acccagcuaa gcuggauaaa uccuuaggga ggaagggcgc caaacagcuc     3300

auucccaaga aggacgauau gccuaccgca cuguauggag gcuacacagg gaguaaagac     3360

gcguucauga gcaucguacg acuuaagaaa aaggaugaag uguauuacaa agucauugga     3420

aucccagugc gcauagccga cucuguucag cacgguaagg agguggaugu cgaggcucug     3480

acucaguuua uugaaaccaa auucacgaag gagaaccuaa auaagaaaac cggcgaaauc     3540

acacggaagg uggagugguu cgaacucguu cugccgaaag uguuguuugg gcaagccguc     3600

uucgacggag gccagcccuu uaugcugggu uccucaacuu auccucauaa cuuaagagag     3660

cucguacuga ccaaggauag ugugaaaaag aucgcaggga aggagagcaa uaaaucguac     3720

cuugacgcuu augacgaaau uauggaggcc augaacaagu acuucccacu guacgauaca     3780

aauaaauuua gggaaaaggu uauagagggc cguuccaaau ucaucgacuu gcccgauaag     3840

gacgaguaug aaaacaauaa gcucgugcag gucggaaaau cuacuauuau cgaucgcaug     3900

uuuauuggcc ugcacgcaaa cgccgcucgg accgaccuaa agaauaucgg gauaacgaca     3960

ccuuucggau uuaugcagca agcgagcggc aucaaacuga ccaaggauac ugugguacuu     4020

uuccagucaa guacaggucu cuuugagaga aaaguggccc ugaaggacuu guga           4074


<210>  3053
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3053
augaaggagc ccuacggcgu ggggcuggac aucggaacca acagcgucgg uuggacaguu       60

guggaugccu ccggccacgu aagaaaaauu aaggggcaga cuggaauagg cgugaggcug      120

uucaaagaag gugcugcagc cgcggaccgg cgcggguuuc gaacgacccg uagaaggcuu      180

aagcggguca aauggcgccu gagauuguua agggaguucu uugaucaacc uaucucuaag      240

guggacauua auuucuuugc ucggcgcaag uauucagaug uuaguccacg agacccgaac      300

uacaauggac uggaaaagac acuauucaac gauagaagcg accaggauuu uuaucaugac      360

uaccccacua ucuaucaccu cagggaggcc cugaugaccc agcaucggaa guucgaugug      420

cgugaaauuu accuugcaau ccaccauaua gucaaauauc gcggcaauuu ucugagaaac      480

gacgccgcua cagcauacag guccggcacc uuggaucucc agcaacacuu cgagacucug      540

aaucaccugu uuucucaggc cgaccucgag uuaaaccuga aucuuacaac cgacguggcu      600

uugcuggauu cgaucaagca gacgcuagua cggacugaca uuucacgaag cgaucgccag      660

aaacucauca ugccucugcu ugccguucug accggggcga caacugcaga aaagaagaga      720

caaaaagccg uggucaccga guucgcuaag gccuuggugg gaaacaaaac aaagauugac      780

gugcucaccc ugacggauau cgacgcaacu gaagcuaaag auugggccuu uaguuuagag      840

gagaaucagg acaagcugcc agguauagaa gauagguucu ccgaggucgg ccagcagauu      900

aucgacgaag uuauucggcu cuacgcgucu gugaaccuug cccaacugau ccccgagggg      960

aaacgcuuca gccaguccau gguagaaaag uauaagagac auggagagga uuugaaacua     1020

cugaaggcau acaucagguc acagagugac gcuaaacgug gccgggccau ucgagcuaca     1080

uaugaccaau acauagaugg ugucaagagc aaacagguga cccaggaggc cuuucagaag     1140

gcacucggga aguucgugga cgccgauguu gaccucaauu cuugggcugc gcaaaucaaa     1200

caggcaauug auuccgaaca guuuaugccu aaacuuagaa cuaaacagaa cggagccauc     1260

ccauaucaag ugcagcagaa ugagcuggac cagauaauuu caaaccaaaa ggaauacuau     1320

cccugguugg gcgaggaaaa cccugucauc aaaaggcgcg gcaaguuccc auacaagcuc     1380

gaugagcugu uuggguuccg ggugccguac uauguaggac ccuuaaucac aaaagagaug     1440

caggcugcca ccgcaaaugc cgguuuugcu uggaugguua gaaaggccga cggcccuauu     1500

acgccaugga acuucgauga caaaguggau agggucgcaa gcgcgaccgc uuuuauccag     1560

cgcaugcaga caacugacac cuaccugauu ggggaagagg ugcuacccgc ccgaagucuc     1620

auauaucaac gguucauggu ccugaaugaa cuuaacaaua uccgugugga ugacggacag     1680

uugacaccua gacagaagca aaggcuguac aaccagguuu uuaaaaagca caagcgcgug     1740

acugcaaaga auauucagga caaccucgua gccgcucggg aguucccaac cgcccccaga     1800

aucacaggcc uggcagaucc gaaggucuuu aauucuuccc uuagcaccua ucaugaccug     1860

aaacaggugu ugccugaagc uaugucggau guuaggaagc aagccgacau cgagaaaauu     1920

auacucuggu cuacuaucuu cgaggauucc acgauuuuca agcagaaacu gacagaaauc     1980

ucaugguuaa ccgcggccca gcugaagcag cuaggucaac uccgauaccg cggguggggc     2040

cagcugcuuc ggaaguugcu gacuggacuc aaagacgaua acaauagaag uauuauggac     2100

ggccuguggg agaccaacgc uaauuuuaug caguuaagga gccagcccgc aaucgaaaac     2160

caaauaaagu ccgccaauca ggcuaaccug acaaccgccg aucuucagga cacuaucaac     2220

gaguuguaua caucuccaca aaauaaaaag gcaauucgug aggugauggu cgugcucgcg     2280

gauaucaaag acgccaugca cgggcagacg ccuaacugga uuuucguaga agcugcccgc     2340

ggagguggcg uggcagggcg gagaacccag agcaggucaa agcagaucgu ugcugccuac     2400

caaggaacug cacaggccau aguccgagau caggugcagc gggagcugaa ggagaaaauu     2460

aagaauaaag cuaacuuuaa uacacgccua gugcuguacu uccuccaaaa cggcagagac     2520

cuguauaccg gugacgcgau caauauugau aggcuuagug aguacgacau cgaucacaua     2580

uugccccagu cccuggucaa ggacgauagc uuagacaacc ggguucucac aaaugcccga     2640

aucaacagag agaaaaauga uacuuuugca ucugaaaagu ucgggaagaa aaugggcgcu     2700

aaguggaaag agaugcaucg caugggacug augaccaaga ggaaacuuga ccaccuguug     2760

augcguccag augaaauuuc aaaguaugcc acgggcuuua ucaaccggca gcucguggag     2820

accagacagg ugauuaagcu gguagaggaa cuaaucaaug ccgaauaccc caguacagcu     2880

auagucagcg ugaaagcaaa ccugacucau caauucaggc agaccuuuga cuucccuaag     2940

auucgcgagg uuaaugauua ucaccaugcc uuugacgcgg cucucacagc cuucaucggg     3000

acuuaccugc uuaaacgaua uccaaaguug gagagauuuu ucguguacgg aaaauucgca     3060

aagcagccgg ucaaccugac ccgguuuaac gugaucagga aauuagccaa gacggacaca     3120

cccauuaccg auaucgacac uggugaaguu cucugggaua agcugacaga cauuaaauac     3180

uucgagaccc uuuaugauua caagcgccug cuagugacuc gugaaguauc cgagaauuau     3240

ggcgcuaugu uugaccagac ccuccugaaa gcaucugaua acgggaagag auugauacgg     3300

aaaaagcaau cguucgacac agccaucuac ggaggcuaua cggguaagac ccuggcguac     3360

auggcuauug ucaaagugag cacuaagaaa gaugugcagu aucaggucgu uggggugccu     3420

acaaccaugg uaacacaaau cgaacaguua gagcagcagg gcaugacuaa uaagcaagcc     3480

gugaccaaag ucauugagcc acucaucgca gccaacaaua agaagaugcu gacguacgaa     3540

gugcuucugc ccaaaguuag guuugaacag gugguccgcg accgaauaaa gggacagaca     3600

cacagauucg cuuugggcac cgauacuuac uaucacaaca uccaggaacu cuaccugccu     3660

cuaacccugc aacgggccuu ucuugggaaa cagaaugagu ccgaggugca gcucaaccag     3720

aaucuguuga caguuuucga cgcaacucug gcucaaguga acagguauuu uccauuauac     3780

gaaaugaaua aguucaaaaa gauucucaac ucagcccgcg aucagaucga gcugcuugaa     3840

gcgaagaauc aguuugucaa aaacaagcug guauugggaa aacaaggcau ucucaccaau     3900

auguucaucg gucugcaugc caacgcaagu uauggggacc uaaaggugcu gggaguuaaa     3960

auagacuucg gcaaacuuca ggugcccggu ggcauuaagc ucacagggga ugcugagauc     4020

aucuaccagu cuccgacggg acuguuugaa agaaaaauug ccuugaagga cuuauga        4077


<210>  3054
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3054
augaacgagc ccuacggcgu ggggcuggac aucggaacca auagcgucgg uuggacaguu       60

guggauauga acggcagagu aaggaaggug aaagggaaga cugcccucgg agcucggcuu      120

uucaaagaag gcgcaacggc cgaggaccgc cgagguuuuc guaccacaag aaggcggcug      180

aagcgcguca aauggagauu gagguuacug cgggaguucu uugaucagcc uauuuccaag      240

auagacccaa auuucuuugc gcgccgaaag uauucugaua ucucaccgag agaccccaac      300

uacaaugggc uagcuaaaac ucucuucaac gauaggaccg accaagaauu uuaucacgau      360

uacccuacaa uuuaucaucu gcgggacaaa cuuaugacca gugaucguaa auucgacauc      420

cgcgaaauuu accuggccau ccaccauaua gugaaguaua gaggaaacuu uuugaggacu      480

ggcccagcaa gccaguacgg cuccgccucu cuccaccugg cuacaucguu ccagaaacug      540

aaugaucucu uugcacaguc agaggagagc uuaaauuuca agcugguuac cgacgaagcc      600

caacuucagc agauccagca aauuuuggug cggacggacc ugagucgauc cgagcagcag      660

cgccagaucu ggccccuaau ggucgugcuc augggggcua cugccgcgga aaagaaaaga      720

caaaaggauu uugcaaaaga gcuggccaac gcucuuguag gacugaaggc caaauugaau      780

guugugaccc ucacaaacgu cgacacuacc guggugaagg auuggacauu cacccuggag      840

gaaucucagg acaaauuacc ugagauugag gaacagcuga gcgaagucgg ucaacagauc      900

auggaugagg uuauacagcu cuacgcaucc gugaaucuug cucagcugau uccagccggc      960

aagagguucu cacaacauau gguagagaag uauaaccacc acgaaaagaa uuuggagcua     1020

cugaaggcgu acauccauag ucagagcgac ucuaaacggg ggcgcgaaau uagagccacg     1080

uaugauaggu acaucgacgg agugaagucc aaacccguca cucaggagau guuuuauaag     1140

gaucucauga aguacgugga agcagacgcu acaucaaacc accuggccgc ugagaucaaa     1200

gaugagauug acagcgaaca guucaugccu aaacuucgua ccaaacaaaa cggcaguaua     1260

ccauaucagg uucagcagua cgagcuggac caaaucauuu cucaucagaa gaaauacuau     1320

ccgugguugg gugaagagaa ucccguggcc gaacggcgag ggaaguuucc uuacaagcuc     1380

gaugagcugg ucggauucag agugccauau uacguaggcc ccuuaaucac uaaagaggac     1440

cagcaggcaa cauccggcgc cggguuugcu uggaugguua ggaaggcgga uggaccuaua     1500

accccaugga acuucgacca aaaaguggau cgcauugcaa gcgccacggc uuuuauccag     1560

cggaugcaga ccacagacac uuaucugauc ggugaagaug uccuacccgc cagaucgcuc     1620

auuuaccaaa gguucauggu gcugaaugag cuuaacaaua ugcgcgucga agaccagaag     1680

uuggcaccgc gacagaaaca gcggcuguau aaccaagugu uuaagcagca ccagcacguu     1740

ucugugaaga auauccagca aaaccucaug gaugccggcg aguaccguga gaccccuaaa     1800

auuacagggc uggcugaccc caagggauuc aauuccucac uuaguacuua ccaugauuuu     1860

aaaaagauac ugccagaagc caucgcagac gagcacaaaa gagcggauau ugaaaagauc     1920

aucuugugga gcaccacauu cgaggacucc gcuauuuuua aacagaagcu cgaagaggua     1980

gccuggcuga ccgacgcaca gaggaagcag uuaucuggcc ugcgcuaucg ggguuggggg     2040

caacuaagcc auaaacuccu gacugccuuc aaggaugaca acggcagauc agucauggau     2100

ggacuuuggg agacgaguga caauuucaug caguugagga aacagccugc uauagaaaca     2160

caaaucaagg aggccaacca ggcaaaucug accgaugcug acauucagga uacuaucaac     2220

gaacucuaca ccagccccca gaauaaaaag gccauucgag aggugaugcu gguuuuagac     2280

gauaucaaga acgcgaugca cggccaaaca ccauccugga uauuuaucga agccgcucgc     2340

gggggaggug uggcaggccg gagaacccag ucuaggucaa aacagauugu cgaggccuau     2400

aagaguacug cuaaagagau cgugagcgaa aagguacagc augagcugaa cgaaaagauu     2460

aaggccaagg cagacuucaa uacacgucuu guguuguacu uucuccaaaa cgggcgcgau     2520

cuguauacga augaggcgau caacauagac cggcuauccg aauacgauau ugaccacauc     2580

cugccccagu cucucguuaa agacgauucg cuggacaaua gaguccuuac cagcgccagg     2640

auuaaccgag agaagaauga uacuuucgcu uccgagaaau uuggacggaa gaugggcgcc     2700

caguggcgcg aauugcacag aaacggucug augacacaga ggaaauuaaa gcaucuccug     2760

augcggccug acgagaucuc aaagcacgca accgauuuca uaaaucgaca acuuguggaa     2820

acaagacagg ugaucaaacu ggucgaggag uugauuaguu cugaauaucc aagcgcuucc     2880

aucguugccg ugaaggcaaa ccucacucau caguuucgcc agaccuucaa uuuuccgaaa     2940

cugagggagg ugaacgacua ccaccacgcc uucgaugcuu cacuaacggc guuuauuggg     3000

auguaccugc ucaagcaaua ucccaaacug gaacguuucu uuguauacgg caaguucgcc     3060

aaacagccua ucaaucuuac ccgguucaac gucauaagaa aguuggcagu ggcugaggaa     3120

ccaauugcca auaucgagac aggagagauc cugugggaca agacugccga uauuaaaugc     3180

uuugaaaagu uauauaacua caaaaggcuc cugguuaccc augaggugcg cgaaaacuau     3240

ggcgcuaugu ucgaccagac aaucuuuaag gcaacugaua gccgaaaaag acuuauucaa     3300

aagaagaaag gguucgccac cgagacguac ggagguuaua caggccggac ccuggcguac     3360

cuagcuauag ucaagguggu uacuaauaaa gacacacagu accagguggu agggaucccc     3420

accacuaugg ucacccagau ucaacagcuc gaagccucug gacugacauc caagcaggca     3480

gugacgaaag ccaucggcgc uaugauuaac gagaauaaca agaaguugca gaguuaugag     3540

gugcuguuac cuaaagucag guuugaacaa guugugcgcg acgagguaaa gggucacauc     3600

caucguuucg cacucgggac cgauacuuac uaucacaaua uacaggaacu guaccuucca     3660

cugacaaucc agagagccuu uuugggcaaa cggaacgaca ccgaugcgca gacagagcaa     3720

aaucuccuga cuguguucga aaccacgcua gcucagguca acagguauuu uccccuguac     3780

gagaugaaua aguucaaaca gauucuuaac agcgcccaag agaaguuugc agcccucccg     3840

acaaagaaua aaaccgugaa gaacaaacug guuacuggaa aggacggcgu guugaauaac     3900

cuguucaucg gguuacaugc uaaugccucu cucggagauc ugaaaauucu uggcaugaag     3960

accgguuucg ggaagcugca ggucccugga ggcuugaaac ucacagacca ggcagaaauc     4020

auauaucagu ccccaacugg ucuguuugag cgcaaggugg cucuaaaaga ucuguga        4077


<210>  3055
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3055
augaacgagc ccuacggcgu ggggcuggac aucggaacca auagcgucgg uuggacaguu       60

guggauauga acggcagagu aaggaaggug aaagggaaga cugcccucgg agcucggcuu      120

uucaaagaag gcgcaacggc cgaggaccgc cgagguuuuc guaccacaag aaggcggcug      180

aagcgcguca aauggagauu gagguuacug cgggaguucu uugaucagcc uauuuccaag      240

auagacccaa auuucuuugc gcgccgaaag uauucugaua ucucaccgag agaccccaac      300

uacaaugggc uagcuaaaac ucucuucaac gauaggaccg accaagaauu uuaucacgau      360

uacccuacaa uuuaucaucu gcgggacaaa cuuaugacca gugaucguaa auucgacauc      420

cgcgaaauuu accuggccau ccaccauaua gugaaguaua gaggaaacuu uuugaggacu      480

ggcccagcaa gccaguacgg cuccgccucu cuccaccugg cuacaucguu ccagaaacug      540

aaugaucucu uugcacaguc agaggagagc uuaaauuuca agcugguuac cgacgaagcc      600

caacuucagc agauccagca aauuuuggug cggacggacc ugagucgauc cgagcagcag      660

cgccagaucu ggccccuaau ggucgugcuc augggggcua cugccgcgga aaagaaaaga      720

caaaaggauu uugcaaaaga gcuggccaac gcucuuguag gacugaaggc caaauugaau      780

guugugaccc ucacaaacgu cgacacuacc guggugaagg auuggacauu cacccuggag      840

gaaucucagg acaaauuacc ugagauugag gaacagcuga gcgaagucgg ucaacagauc      900

auggaugagg uuauacagcu cuacgcaucc gugaaucuug cucagcugau uccagccggc      960

aagagguucu cacaacauau gguagagaag uauaaccacc acgaaaagaa uuuggagcua     1020

cugaaggcgu acauccauag ucagagcgac ucuaaacggg ggcgcgaaau uagagccacg     1080

uaugauaggu acaucgacgg agugaagucc aaacccguca cucaggagau guuuuauaag     1140

gaucucauga aguacgugga agcagacgcu acaucaaacc accuggccgc ugagaucaaa     1200

gaugagauug acagcgaaca guucaugccu aaacuucgua ccaaacaaaa cggcaguaua     1260

ccauaucagg uucagcagua cgagcuggac caaaucauuu cucaucagaa gaaauacuau     1320

ccgugguugg gugaagagaa ucccguggcc gaacggcgag ggaaguuucc uuacaagcuc     1380

gaugagcugg ucggauucag agugccauau uacguaggcc ccuuaaucac uaaagaggac     1440

cagcaggcaa cauccggcgc cggguuugcu uggaugguua ggaaggcgga uggaccuaua     1500

accccaugga acuucgacca aaaaguggau cgcauugcaa gcgccacggc uuuuauccag     1560

cggaugcaga ccacagacac uuaucugauc ggugaagaug uccuacccgc cagaucgcuc     1620

auuuaccaaa gguucauggu gcugaaugag cuuaacaaua ugcgcgucga agaccagaag     1680

uuggcaccgc gacagaaaca gcggcuguau aaccaagugu uuaagcagca ccagcacguu     1740

ucugugaaga auauccagca aaaccucaug gaugccggcg aguaccguga gaccccuaaa     1800

auuacagggc uggcugaccc caagggauuc aauuccucac uuaguacuua ccaugauuuu     1860

aaaaagauac ugccagaagc caucgcagac gagcacaaaa gagcggauau ugaaaagauc     1920

aucuugugga gcaccacauu cgaggacucc gcuauuuuua aacagaagcu cgaagaggua     1980

gccuggcuga ccgacgcaca gaggaagcag uuaucuggcc ugcgcuaucg ggguuggggg     2040

caacuaagcc auaaacuccu gacugccuuc aaggaugaca acggcagauc agucauggau     2100

ggacuuuggg agacgaguga caauuucaug caguugagga aacagccugc uauagaaaca     2160

caaaucaagg aggccaacca ggcaaaucug accgaugcug acauucagga uacuaucaac     2220

gaacucuaca ccagccccca gaauaaaaag gccauucgag aggugaugcu gguuuuagac     2280

gauaucaaga acgcgaugca cggccaaaca ccauccugga uauuuaucga agccgcucgc     2340

gggggaggug uggcaggccg gagaacccag ucuaggucaa aacagauugu cgaggccuau     2400

aagaguacug cuaaagagau cgugagcgaa aagguacagc augagcugaa cgaaaagauu     2460

aaggccaagg cagacuucaa uacacgucuu guguuguacu uucuccaaaa cgggcgcgau     2520

cuguauacga augaggcgau caacauagac cggcuauccg aauacgauau ugaccacauc     2580

cugccccagu cucucguuaa agacgauucg cuggacaaua gaguccuuac cagcgccagg     2640

auuaaccgag agaagaauga uacuuucgcu uccgagaaau uuggacggaa gaugggcgcc     2700

caguggcgcg aauugcacag aaacggucug augacacaga ggaaauuaaa gcaucuccug     2760

augcggccug acgagaucuc aaagcacgca accgauuuca uaaaucgaca acuuguggaa     2820

acaagacagg ugaucaaacu ggucgaggag uugauuaguu cugaauaucc aagcgcuucc     2880

aucguugccg ugaaggcaaa ccucacucau caguuucgcc agaccuucaa uuuuccgaaa     2940

cugagggagg ugaacgacua ccaccacgcc uucgaugcuu cacuaacggc guuuauuggg     3000

auguaccugc ucaagcaaua ucccaaacug gaacguuucu uuguauacgg caaguucgcc     3060

aaacagccua ucaaucuuac ccgguucaac gucauaagaa aguuggcagu ggcugaggaa     3120

ccaauugcca auaucgagac aggagagauc cugugggaca agacugccga uauuaaaugc     3180

uuugaaaagu uauauaacua caaaaggcuc cugguuaccc augaggugcg cgaaaacuau     3240

ggcgcuaugu ucgaccagac aaucuuuaag gcaacugaua gccgaaaaag acuuauucaa     3300

aagaagaaag gguucgccac cgagacguac ggagguuaua caggccggac ccuggcguac     3360

cuagcuauag ucaagguggu uacuaauaaa gacacacagu accagguggu agggaucccc     3420

accacuaugg ucacccagau ucaacagcuc gaagccucug gacugacauc caagcaggca     3480

gugacgaaag ccaucggcgc uaugauuaac gagaauaaca agaaguugca gaguuaugag     3540

gugcuguuac cuaaagucag guuugaacaa guugugcgcg acgagguaaa gggucacauc     3600

caucguuucg cacucgggac cgauacuuac uaucacaaua uacaggaacu guaccuucca     3660

cugacaaucc agagagccuu uuugggcaaa cggaacgaca ccgaugcgca gacagagcaa     3720

aaucuccuga cuguguucga aaccacgcua gcucagguca acagguauuu uccccuguac     3780

gagaugaaua aguucaaaca gauucuuaac agcgcccaag agaaguuugc agcccucccg     3840

acaaagaaua aaaccgugaa gaacaaacug guuacuggaa aggacggcgu guugaauaac     3900

cuguucaucg gguuacaugc uaaugccucu cucggagauc ugaaaauucu uggcaugaag     3960

aucgguuucg ggaagcugca ggucccugga ggcuugaaac ucaccgacca ggcagaaaua     4020

auuuaucagu ccccaacagg ucuguuugag cgcaaggugg cucuaaaaga ucuguga        4077


<210>  3056
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3056
augaaggagc ccuacggcgu ggggcuggac aucggaacca acagcgucgg uuggacaguu       60

guggaugccu ccggccacgu aagaaaaauu aaggggcaga cuggaauagg cgugaggcug      120

uucaaagaag gugcugcagc cgcggaccgg cgcggguuuc gaacgacccg uagaaggcuu      180

aagcggguca aauggcgccu gagauuguua agggaguucu uugaucaacc uaucucuaag      240

guggacauua auuucuuugc ucggcgcaag uauucagaua ucaguccacg agacccgaac      300

uacaauggac uggaaaagac acuauucaac gauagaagcg accaggauuu uuaucaugac      360

uaccccacua uuuaucaccu cagggaggcc cugaugaccc agcaucggaa guucgauguu      420

cgugaaaucu accuugcaau acaccauauc gugaaauauc gcggcaauuu ucugagaaac      480

gacgccgcua cagcauacag guccggcacc uuggaucucc agcaacacuu cgagacucug      540

aaucaccugu uuucucaggc cgaccucgag uuaaaccuga aucuuacaac cgacgucgcu      600

uugcuggauu cgauuaagca gacgcuagug cggacugaca ucucacgaag cgaucgccag      660

aaacucauua ugccucugcu ugccguacug accggggcga caacugcaga aaagaagaga      720

caaaaagccg uugugaccga guucgcuaag gccuuggucg gaaacaaaac aaagaucgac      780

gugcucaccc ugacggauau agacgcaacu gaagcuaaag auugggccuu uaguuuagag      840

gagaaucagg acaagcugcc agguauugaa gauagguucu ccgagguggg ccagcagauc      900

auugacgaag ucauccggcu cuacgcgucu guuaaccuug cccaacugau ccccgagggg      960

aaacgcuuca gccaguccau gguggaaaag uauaagagac auggagagga uuugaaacua     1020

cugaaggcau acauuagguc acagagugac gcuaaacgug gccgggccau acgagcuaca     1080

uaugaccaau acaucgaugg uguaaagagc aaacagguga cccaggaggc cuuucagaag     1140

gcacucggga aguucgucga cgccgaugug gaccucaauu cuugggcugc gcaaauuaaa     1200

caggcaaucg auuccgaaca guuuaugccu aaacuuagaa cuaaacagaa cggagccaua     1260

ccauaucaag uucagcagaa ugagcuggac cagauuauca acaaccaaaa ggaauacuau     1320

cccugguugg gcgaggaaaa uccugugauc aaaaggcgcg gcaaguuccc auacaagcuc     1380

gaugagcugg ucggguuucg ggugccguac uauguaggac ccuuaauuac aaaagagaug     1440

caggcugcca ccgcaaacgc cgguuucgcu uggaugguua gaaaggccga cggcccuauc     1500

acgccaugga auuuugauga caaaguggau agggucgcau cagcgaccgc uuucauucag     1560

cgcaugcaga caacugacac cuaccugaua ggggaagagg ugcuacccgc ccgaagccuc     1620

aucuaucaac gguuuauggu ccugaacgaa cuuaauaaca uucgugugga ugacggacag     1680

uugacaccua gacagaagca aaggcuguac aaucagguuu ucaaaaagca caagcgcgug     1740

acugccaaaa acauccagga caaucucgua gccgcuggcg aguuuccaac cgcaccccgg     1800

aucacagguc uggcagaucc gaaggucuuc aacaguucuc uuuccaccua ucaugaccug     1860

aaacaggugu ugccugaagc uaugagcgau guuagaaagc aagccgacau ugagaaaaua     1920

auccucuggu cgacuauuuu ugaggauucu acgaucuuca agcagaaacu gacagaaauu     1980

uccugguuaa ccgcggccca gcugaagcag cuagggcaac ucagguaccg aggcugggga     2040

cagcugucac gcaaacuuuu gacuggccug aaagacgaua auaaccggag uaucauggac     2100

gggcucuggg agaccaaugc uaacuucaug cagcugagaa gccagcccgc aauagaaaau     2160

caaaucaagu ccgccaacca ggcuaacuua acaaccgccg aucugcagga cacuauuaau     2220

gagcuuuaua caucuccaca aaacaaaaag gcaaucaggg aggugauggu cguguuggcg     2280

gauauuaaag acgccaugca cggacagacg ccuaauugga ucuuuguaga agcugcccgu     2340

gguggcgggg uggcaggacg ccggacccag agcagaucaa agcagauagu ugcugccuac     2400

caaggcacug cacaggucau ugugagugau caggugcaga gggagcucaa ggaaaagauc     2460

aagaacaaag ccaauuucaa cacacgacug guccuauacu uucugcaaaa uggucgggac     2520

cucuauaccg gggacgcuau uaacaucgau cgccuguccg aguacgacau agaucacauc     2580

cuuccccaga gcuugguuaa ggacgauucu cuggacaaua gaguguuaac aaacgcgagg     2640

auuaaucggg aaaagaacga uacuuucgcc ucagagaagu uuggcaagaa aaugggagca     2700

aaguggaaag agaugcaucg aaugggccuc augaccaaga gaaaacugga ccaccuucug     2760

augcgcccag augaaaucag uaaguaugcu acgggguuca uuaauaggca guugguggag     2820

acccgucagg uaaucaagcu cgucgaggaa cugauaaacg ccgaauaccc cagcacagcc     2880

auuguguccg uuaaagcuaa ucuaacucau caauuucggc agaccuucga cuuuccuaag     2940

aucagagagg ugaacgauua ucaccaugca uucgacgccg cgcugacagc uuuuaucgga     3000

acuuaccucc ugaaaaggua uccaaaacuu gagcgcuucu uugucuacgg uaaauucgcc     3060

aagcagccgg ugaacuugac ccgauucaau guuauuagaa aacuggcaaa gacggacaca     3120

cccaucaccg auauugacac uggcgaagug uuaugggaua agcucacaga cauaaaauac     3180

uuugagaccc uguaugauua caagcggcuu cugguaacua gggaagucuc ugagaacuau     3240

ggggccaugu ucgaccagac ccuacucaag gcuucggaua auggaaagcg ccugauccgu     3300

aaaaagcaga gcuuugacac agcaauuuac ggcgguuaua cggggaagac cuuggccuac     3360

auggcgaucg ugaaaguguc cacuaagaaa gauguccaau aucagguugu gggcguaccu     3420

acaaccaugg ugacacaaau ugaacagcug gagcagcagg gaaugacuaa caagcaagcu     3480

gucaccaaag ugaucgagcc auuaauagcc gcaaauaaca agaagaugcu cacguacgaa     3540

guucugcuuc ccaaagugag auucgagcag gucgugcggg acaggaucaa gggccagaca     3600

caccgcuuug cccuggggac cgauacuuac uaucacaaua uucaggaauu guaccucccu     3660

cugacccuac aacgagcuuu ccugggaaaa cagaacgagu cagagguuca gcuuaaucag     3720

aaccuccuga caguguuuga cgccacuuug gcacaaguca auagauauuu cccacuguac     3780

gaaaugaaca aguuuaaaaa gaucuuaaau agugcucggg aucagauuga gcuccuggaa     3840

gccaagaacc aguucguaaa gaauaaacuu gugcugggca aacaagguau cuugaccaac     3900

auguuuauag ggcuccaugc gaacgccucu uucggagacc uaaagagccu gggcguuaaa     3960

acauccuucg gucagcuuca ggugcccggc gggauuaaac ugacgggaga cgcagagauc     4020

aucuaucagu caccgaccgg ccucuuugaa aggaagauug cucugaaaga uuuguga        4077


<210>  3057
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3057
augaagcagg gcaaaagagg guacuaucug ggacucgaca ucgguaccga uagcauuggc       60

uacgccguga cagacgagca auaucaccuu cugagguucc augggaacga ugcuugggga      120

ucccacgucu uugacucugc aucauugugc gaugaacggc gcggcuuccg aagugcccgu      180

agaagguuag accggcgcca gcagagaguu aagcugcuac aggagauauu ugcgaaagaa      240

aucgcuaaua uugaucccag guucuuuauc cggcucagcg aguccuaccg cuggcgagac      300

gauacugacg auagauauau uuacuucaac gaagaggagu auacggacau ccaauacaug      360

agggauuacc cuaccauaca ucaccugauc ugugaacuua uggagaauga agaggagcau      420

gacauucggc ugguguauuu ggccugcgca uggcucguag cccaccgugg ucacuuucug      480

ucucagcugu cgguggacaa caucgauuca cucauugaca ucgaaagcgu cuaccagaag      540

uucuuacauu ucuucauaga uaaugggugu gagaacccau ggauucagga cgcugaacug      600

gugggaggca uccuuaaaga gcacauuggc guuacagaaa agcauaaucg cuugggggag      660

aucaugcugg gaaacagaaa gccgaguaaa gaugugaggg acacuuuucu auauuccgag      720

uacugcauca uucggcuccu ggcugguggc accuguaaac uucgagaucu guaugggaaa      780

gaagaguacg aagacuucgg aucugucagc uuggagaugg augacgauaa gcucgccgaa      840

auaaugauca auauuggcga ggacuaugag cugaucgcug uguuacgcuc ccugcacgac      900

uggucaguac ucgccgauau acuugacggu caaaacgggc ugacaaauau uaguaccgcg      960

aaaguugcaa ucuacgaaca gcacaagaag gauuuggcca cucuaaaaua cuuuaucaga     1020

aaguauugcc ccgagaaaua caacgcuauu uucaaggacg ccagggaaga uaauuaugug     1080

gcauacagcu aucauuacaa caauaaacug gacggacaga ucaagaaaaa ggcugauaca     1140

gagaccuucu cuaaguuucu cauuaaaaua auccacucca uuaagccuga caaagaagau     1200

gagaucaucu aucaggacau ggauaaccgg cugggccuuc gccaauuccu gccaaagcag     1260

agaaacacgg acaauagggu cauuccccau caguuguacg aguacgaacu caaaaagaua     1320

cuggagaacg ccgcgaagua uuuaucauuu cugaaucaaa aagaagaugg caucagcaac     1380

acugagaaga uuaucaguau uuucaaauuu aagaucccuu acuauguggg gccacuaaau     1440

aaacacucug aguucgccug gauagaccgu aaguccggaa agauccuccc cuggaacuac     1500

gaaaauauga uugacgauga cgcaagcgag gaggcuuuua ucaaaaagau gaccaaccag     1560

uguacauauc ugccugguga aucggugcuu ccaaaagauu cuuugugcua ccagaaguuc     1620

augguccuga augaaauuaa caauaucaaa guugacggcc ggaagauauc cgugggggcc     1680

aagcagggaa uuuauucaga ucucuuucug aaauacaaga aaguaaagcg aagucaaauc     1740

gaggaguacc uuauuagcaa cggccagcug gaaaagaaua gaagggagac uuuguccggu     1800

aucgacauac agaucaaguc uagccucuca agucauauug cuuuccgccg gcuguuagaa     1860

cagaaaaucc ugaacgaggc cgauguggaa agaauuaucg agagggcauc cuaugccgag     1920

gacaaggggc gcgucgcuaa guggcuacga gcgaaauacc cgcaccucac cgaagcagau     1980

auaaaguaua uuuguaaagu gaagaucaaa gacuuuggcc ggcugagccg uacauuccuu     2040

accggaaucg agggcgccug caaggaaacg ggggagguug cuacuauuau cucuaugaug     2100

ugggaaucaa augauaacuu gauggagcug cucagugaga gauacacauu ugccgacacc     2160

auucuggaau uuaagagcga uuauuacacu gagaggaaac aaacauuauc cgaacgccug     2220

gacgagaugu augugucuaa cgcaguccgg agacccauau acaggacccu ugacaucgug     2280

aaggauuugg agaaagccuu cggaaagccu gagaaaauuu uuaucgaaau gacgcgaggu     2340

gcuaugccag aacucaaggg caagcgcacc aaaucgcggc agcagcagcu gcuagaguac     2400

uaugacaagu guaaagaaga ggauguaaga gaccugaagc aacagcucga ggcccugggg     2460

gaauacguug auaauaaacu ucagagcgac agguuguucc uguauuuuau gcaguucgga     2520

aagugcgcau acuccggcau gcccauuuca uuagagcguc ucauggcggg uaguaaggaa     2580

uaugauaucg accacauaua cccucaagca uaugugaaag augacucuau cauuaacaau     2640

aagguccugg ugcuuagcgu cgccaacggg gagaaaaagg auguguaccc aaucaaaucc     2700

gaaauucgca auaagaugca gaaaacaugg ucauuuuggc aucacguugg cacuaucagc     2760

gacgagaagu acaagcggcu gauaagaucu acccccuuca cagaggacga aaaauaugga     2820

uuuauuaaca ggcaguugac ugagaccucc caaaguacaa aggugguagc agaacuccug     2880

aaagagcgau acccggaagc cgagaucguc uauagcaagg cuggccuaau cucugauuuc     2940

cggcaugagu uugaccugua caaaucccgc ucauauaaug aucuccacca cgccguggac     3000

gcauaccuga acauuguugu ggggaauguc uaucauauga gauucaguag gcagugguuu     3060

aacgugaaua gcucguacuc cauaaagacc aagacucuuu ucacgcacac aguaaacugc     3120

aauggaaaag aaguguggaa caaggauaug uugccuggug uucugaaaac cgcuaagaaa     3180

aauacugccc auuuuaccaa gcacgcgaca uucaagaccg gcggguuauu cgaccagaac     3240

cccgucaaaa aggccccagg acucacuccu cugaaagcug gccuucccac agagaaguac     3300

gguggguaua acaaagcagg cgugauguuu uucaucccag ugcgguacaa gucuggaaag     3360

aaaacggucc ugaugguucu aagcguggaa cuccuguaug gcaaucgauu uuuggaggau     3420

gagauuuucg ccaaggaaua cgcuaaagac agacugcagc gcaugaucgg gaagucagug     3480

gaugagauug acuuucccau cggaaugagg ccuuggaaag uaaacaccau auuaagucuc     3540

gauggcuucc guaucuguau uacugguaau gccuccgggg gaaagugccu gaucgcacaa     3600

ccaauuaugc aguuuucuag cgacgaauau uggaaguacu aucuuaaaaa ggucgagaaa     3660

uucguggaaa agguuaaaaa caauuccuca uacguguacg augucgacua ugacgcggug     3720

cagcaugagg auaaccugaa guuguacgac cucuaucugg auaaacuaca gaauagcauc     3780

uaccggaaga gaauaaacgc cccgauucaa acacugaucg agggcaggga aaaguuuauc     3840

gacuguagug uuauugagca gugccaggug cuucucaaua uccaccaggu auucggucgc     3900

augaccucug gcugugaucu gacauugaua gggggaaaaa cucgauccgc uagcaccguc     3960

aacuuuucgu caauuauccu gaauuggaag aaaaacuaua cggacgugag aauuaucgau     4020

caaucugcca guggcuuaug ggaagugguc uccgagaaua uacucgaaua ccuguga        4077


<210>  3058
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3058
augggcaaga uguacuaucu ggggcucgac aucggaacca acagcguggg uuacgccguc       60

acagaucccu ccuaucaccu ucugaaauuc aagggcgagc cuaugugggg agcucauguu      120

uuugcagccg ggaaucaguc ugcggaaaga aggucauucc ggacuagccg ccgacguuug      180

gacagaaggc aacagcgggu gaaauuagua caggagauuu uugcuccagu gauaagcccg      240

aucgaucccc gcuucuuuau uagacugcac gaauccgccc uauggaggga cgaugucgca      300

gagacggaca agcauaucuu cuuuaacgau ccuaccuaca cagacaaaga auauuacucu      360

gauuauccaa cuauucacca ucucaucgug gaccugaugg agucgucaga gaagcacgau      420

ccccggcuug uuuaccuggc cguggcuugg uuggucgcac accgcggcca uuuccucaau      480

gaaguggaca aggauaacau aggugacgua cugagcuuug acgccuucua cccugaguuu      540

cuggcuuucc ucagugauaa ugggguuucc ccaugggugu gcgaaucuaa agccuuacag      600

gcgacccugc uuagccgaaa cuccgucaau gacaaguaua aagcauugaa gucacugauc      660

uuuggaaguc aaaaacccga ggauaacuuc gacgccaaua uuagcgagga uggccuaauc      720

cagcuccugg cuggcaagaa ggugaaagug aacaaacuuu ucccucagga aucuaaugac      780

gccuccuuua cacugaacga uaaagaggac gcaauugaag agaucuuggg gacccucacu      840

ccagaugaau gugaguggau agcucacauu agaaggcugu ucgacugggc caucaugaag      900

caugcguuaa aagauggacg gacaauuuca gagagcaagg ucaaacugua cgaacagcac      960

caccaugacc ucacccaacu uaaguauuuu guuaagacgu accuggccaa agaguaugac     1020

gauaucuucc guaaugugga cagugaaacu accaagaacu acguagcaua uucuuaccac     1080

gugaaagagg ucaaggguac auugccgaaa aauaaggcua cucaggaaga guuuugcaag     1140

uacgugcuag gcaaaguuaa gaacaucgag uguuccgaag ccgauaaagu ggacuucgau     1200

gagaugauuc agcgccugac cgacaacagc uuuaugccca agcaggucuc gggggaaaau     1260

agagugauac cuuaucaacu cuacuauuac gagcugaaaa caauccuuaa caaggcugcc     1320

ucuuaucugc cauucuugac ccagugcgga aaggaugcaa uuuccaauca ggacaaacuc     1380

cugucaauca ugacguuuag gauacccuac uucguaggcc cuuuacggaa ggauaacagu     1440

gaacaugccu ggcuggagcg aaaagcuggu aagauuuauc cauggaauuu uaacgacaaa     1500

guugaucuag acaagagcga ggaagcguuc auccgcagaa ugacuaauac auguaccuac     1560

uaccccgggg aggaugugcu cccgcuggac ucccuuaucu augaaaaguu uaugauuuug     1620

aacgagauca auaacauuag gauagacgga uacccuaucu cugucgaugu gaaacaacag     1680

gucuucggcc uguucgagaa gaaacggcgc gugacuguua aggacauuca gaaucuccug     1740

cuuagccugg gcgcauugga uaaacacggg aagcucacag gaaucgacac cacgauccau     1800

ucaaacuaua auaccuacca ccacuuuaaa agucugaugg aaagaggugu guuaacaagg     1860

gaugacguag agcguauugu cgaacggaug acuuauuccg augacaccaa gcgagugaga     1920

cuguggcuaa acaauaacua cggcacacuc acugccgaug acguuaagca uauaagcagg     1980

cugcgcaaac acgauuucgg gcggcuuucu aagauguuuu ugaccggacu gaaaggcgug     2040

cauaaggaga caggugaaag agcuucaauc cucgacuuca uguggaacac caaugauaac     2100

cugaugcagu uacugaguga gugcuauacu uuuagcgacg agauuacgaa acuucaagaa     2160

gccuacuacg caaaggccca guugucccuc aaugacuucc uggauucuau guauaucucg     2220

aacgcuguca agaggcccau uuaccgcaca cuagccgugg uaaaugacau ccgaaaagca     2280

ugugggaccg cgccaaagcg gauauuuauc gagauggcuc gugauggcga aagcaaaaag     2340

aaaagauccg ugacuaggcg cgagcagauu aagaaccugu aucggucaau cagaaaggac     2400

uuccagcaag aaguugauuu ucucgagaaa auucuggaga auaagaguga cggacagcuu     2460

cagucugaug ccuuguaccu guauuucgca caguuaggca gggacaugua caccggggau     2520

ccuaucaaac ucgaacacau aaaggaccaa aguuuuuaua acauugauca caucuacccc     2580

caguccaugg ucaaagacga cucacuggau aauaaggugc uugugcagag cgagauuaac     2640

ggagaaaagu cuucccgaua cccacuggac gccgcuaucc gcaauaaaau gaagcccuug     2700

ugggaugccu auuacaacca uggucucaua agucugaaaa aguaucagcg gcuaacaaga     2760

agcaccccuu ucacugacga ugagaaaugg gacuuuauca auaggcaacu ggucgaaaca     2820

cgucagucua cgaaggcacu cgcuauucug cuuaaacgca aguucccaga uaccgagauc     2880

guuuacucca aggccggcuu gucaagugac uuccggcacg aguuugggcu ggugaaaagc     2940

agaaacauua augauuuaca ucacgcgaag gacgccuucc ucgcuaucgu gacuggaaac     3000

guauaucaug aaagguuuaa ccgacggugg uucaugguca aucagccgua cucggugaaa     3060

acaaagaccc uguuuacaca cuccauaaag aacggcaauu ucguugcaug gaacggugag     3120

gaagaucuug ggcgcauugu gaagaugcug aagcaaaaua aaaacacuau ccacuuuacc     3180

agauucucuu uugacaggaa ggagggccua uucgauaucc agccccucaa agccagcacg     3240

ggacuggucc cucggaaggc uggcuuggac gugguuaaau augggggaua cgacaaguca     3300

accgccgcau acuaucuguu agugcgauuu acacucgagg auaagaaaac ucagcauaag     3360

cugaugauga uuccaguaga aggucuuuac aaagcgagaa ucgaccacga uaaggaguuc     3420

cugaccgacu augcccagac aacuauuagu gaaauauugc aaaaagauaa gcagaagguc     3480

aucaauauua uguuccccau gggcacccgc cauaucaaac ucaacuccau gauuucuauc     3540

gacggguuuu accugagcau aggaggcaag uccucaaaag guaagagcgu gcuaugccac     3600

gcuauggugc cucugaucgu cccacauaaa auugaguguu auaucaaggc cauggaaagu     3660

uucgcaagga aguuuaaaga gaauaacaaa cuucguauug uugagaaguu cgauaagauc     3720

acgguggaag acaaucucaa ccuguacgag uuguuucugc agaaauuaca gcacaauccc     3780

uauaacaagu ucuuuucuac acaauucgau guacucacca augggcgguc cacuuuuaca     3840

aaacugagcc cggaagaaca ggugcagacc cuucugaaca uauugucgau uuucaagacu     3900

ugcagaucau cuggcuguga ccucaagagu aucaacggau ccgcucaggc cgcaaggauc     3960

augauuagcg ccgaucugac cggccuaucu aaaaaguaca gcgacauacg ccuggucgag     4020

caauccgcuu cagggcuuuu ugugaguaaa agccagaauc uccuggagua cuuguga        4077


<210>  3059
<211>  4083
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3059
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uugguagcaa cuccauaggc       60

uucgccgcug ucgaugaaaa uucucaccuc aucagacuua aggggaaaac aguuauugga      120

gcaaggcugu uugaagaggg caaggccgcg gcugaccggc gcgccucacg aacuacgcgu      180

agaagguuga gucggaaccg cuggagacug agcuuccuga gggacuuuuu cgaaucccau      240

aucaccccua cagauccaaa cuuuuucaug cggcagaaau auucugagau uucgccgaag      300

gauaaggcac gcuacaaaua ugaaaagcga cuauuuaaug acagaacuga ugccgaguuc      360

uaccaacagu aucccaccau guaccaccuc aggacacggc ugcuuaccga cccuucaaaa      420

gcugaugugc gugagaucua uuuugcaaua caucacaucu uaaagagccg cggucauuuc      480

uugacaagug gguacgccaa agacuuuaau acuaacaagg uagcucucaa ugaaauuuuc      540

ccagcccugc aggaugcgua cgcacaggug uaucccgacc uggauaucac cuuugacgag      600

aacaagguca augaguucaa aacggugcuc uuagacgaga aggccacucc uuccgauacc      660

caaagagcuc ugguuaaccu uuugcuggcc gaggacggag auaaagacau ucuaaagcag      720

cagaaacagg ugcucacaga guucgcaaag gcugucgugg gccugaaaac uaaacuuaau      780

guagcccugg gcaccgaagu ugauucuagc gaagcgacag ccuggaacuu uuccuugggg      840

caacucgacg auaagugggc aggaaucgag ucagcuauga ccgacgaagg uacggagaua      900

cuggaucaga uuagggacuu auaccgggcc cgacugcuca auggcaucgu gccagcuggg      960

aaaacucuua gucaggccaa ggucgaugac uaugagcagc accacgaaga ccuggaguug     1020

uucaaagauu accuaaagca acuggaagac gauggaaugg caaaagccau ucgccagcuc     1080

uaugacagau acaucgaugg cgacgaugcu aagcccuuug ugagggagga cuucgugaag     1140

gcgcugacaa aagaagucac cgcacauccu aacgccaaga gcccagagcu ucuggagcgg     1200

uugguucagc cggauuuuau gcucaaacaa cgcaauaagg cuaacggugc cauccccgug     1260

cagaugcagc agagagaacu ggaccaaauu auaaagaacc agucuguaua uuacgauugg     1320

uuagcagccc cuaauccagu ggagaagcac aggcagucca ugcccuacca guucgacgaa     1380

cugcuaaacu uucguauccc uuauuacguc gggccacucg ugacugcuaa ggagcaaaaa     1440

gccgcacagg gaggcguuuu cgcguggaug gugcggaagg aucccgacgg caauauuaca     1500

ccguauaacu uugacgaaaa agucgaucga gaggcuucag ccaauaccuu cauccagaga     1560

augaagacga ccgacacaua ccugauaggg gaggaugugc uuccuaaaca gagcuugcug     1620

uaucaaaguu acgaaguacu caacgagcug aauaacguua gggugaauga caacaaacuu     1680

ucuuuugauc agaagcagca gguccuggga gaauuguucg agcgccauaa uacugugacc     1740

auuaaacaau uugucgacaa ccuccuggca cacggugauu augaccggau ccccgaggug     1800

agaggcuuag ccgaugaaaa gagguuccug uccagccuau cgacauacaa ucagcucaaa     1860

gggauccugc acgaggcuau ugacgauucu gacaagcaga cugauaucga aaagauuaua     1920

gccuggucca ccaucuucga ggacaaggca auuuuuaaaa caaaacuuaa cgaaaucuca     1980

ugguugaaug cuggaaugau caacagucug gccaacauuc gcuaccgagg cuggggucaa     2040

uucagccaua agcuccugaa ugaguuaacc cuguccacug ggcggacggu uuuugaggaa     2100

cuuuugauau cuagccacaa ccucaugcag auccugacag acgaggugcu aaaacagaag     2160

auugcggaac ugaaugccca ugagcucaag gcuucaggca ucuaugcagc cauugaugac     2220

gcuuacacca guccauccaa caagaaagcc cugcgucagg uacuuagcgu caucgaugac     2280

auaacuaaug cagcgggaca agccccuaac ugguuguaua ucgaaaccgc ugauggcgac     2340

ggggugcccg gaaagagaac aaauucuagg cagcgcaaga uucaggagau cuacguuaac     2400

gccgcacagg agcugauuac cgguucagug cggggcgaau uagaggauaa aaucgcuagu     2460

agagccgacu ucacugauag gcucguccug uauuuuaugc aagggggacg agacauauac     2520

acaggcgaac cacuuaauau ugauaaccug agcuccuaug acaucgacca cauuuugccc     2580

cagucucuca ucaaggauaa uucgcuggac aaccgcgugc uaguacaucc ucuggugaau     2640

cgggagaaag auacgauauu cgcaagcacc agauuugcca agcagaugaa cgguaugugg     2700

aggcaguggc acgcugcggg gcucaucucc ucacguaaac ugcgcaaucu ucaaaugcgg     2760

ccagacgaaa uugauaagua cgccaguggc uucguugcaa gacaguuggu cgagacuagg     2820

cagaucauua agcugacaga gcaaaucgug gcugaccagu acccggaugc caaaauaauu     2880

gccgugaagg cuggauuauc uagccagcuc cgaaaagaac uggacuuucc caagaaccgg     2940

gaggucaacc acuaucauca cgcauucgau gccuuucuug cggcucgcau cggcaccuac     3000

cugcuaaaaa gauauccuaa ucucgaacca uucuuuacau acgggaaguu caagaaaacu     3060

gagguuaaga aacugaaguc cuucaacuuu aucagggaca ugacccaugc caaagauaag     3120

auuguggcaa aagagacggg agaaaucgug ugggacaaug ccucagauau uaacgaguug     3180

gaccggauau auaauuucaa gcgaaugcug aucacccacg aaguaagauu ugagacagcu     3240

agcuuauuca agcagacucu cuacgcagcc aaaaacucua agaaucgcgg uggcuccagg     3300

caacugauuc ccaaaaagaa aggguaucuu gucgacaucu acggaggcua cacccaggaa     3360

acagguaguu aucugagcgu gguucguuug acuaagaagg cgauguacgc uguggucaaa     3420

gugucuaccc gggaugccgc aaagcucgcc guugcuaaau ccauuucaga gcagaaggag     3480

aacgaaacgc ugaaaaagau cauagacggg aagcuaagua aaacaagcaa gaaaggcaag     3540

accacucacc agcuguuuga gaucgugcuu ccuagaguag gacagaaaac acucuucaaa     3600

aauucgaagu auaaccaauu ucuggucaau uccgauaccu acaugcauaa cuaucaggaa     3660

uuguggaugc caagggagua ccagcgcaug uggaaagaca uucuguuauc uaaucacggc     3720

gaugcccaac ucgaagggca gcuggaccag aucuucaagu uuauugugag ccaggugaac     3780

ucauauuuca aucuuuacga uaucaaccaa uuucgaaaga agauagcaga cgcuacugau     3840

aaauucgcca agcugcccac ccaugacaca gauaacgcga gaggaaagau ugccacgauc     3900

ggccaguugc ucaucggucu gcaggcaaau gcuaugcgga gugaccuaag gaaccuggau     3960

auuaaaaccc cguucgggcu ucuccaaaua ggaacuggca ucacacugga cuuggacacc     4020

uccauuaucu accagucucc uacagguuua uuugagcgcg aggucccacu gacugaucuc     4080

uga                                                                   4083


<210>  3060
<211>  4083
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3060
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcuc cucuauaggu       60

uucgccgugg ucgaugcuaa uucacaccuu cugagauuga aaggcgagac agcaaucggg      120

gccagguuau uucgggaagg agagagugcg gcuggccgcc gagccagccg uacuacgaga      180

aggcggcugu cccauaaccg cuggagacua agguuccucc aagacuuuuu cgauccucac      240

auuucuucga ucgacccaga uuucuuccug cggcagaagu auucagaaau uagcccgaaa      300

gacaaggagc gcuuuaaaua cgaaaagcga cuuuucaaug auagaaccga cgcacaguuu      360

uauaagcagu accccacaau guaucaucug agguugcacc ucaugaacga ugccaguaaa      420

gcugacccuc gggagaucuu cuuugcaaua caucacaucc ugaagucccg uggucacuuc      480

cugacuccag gggaugccaa aaauuuuaac accgacgaug uugaccucaa gcauauuuuc      540

cccgcuuuaa cagaggccua cgcgcaagca uauccugacc ugacccuuuc uuucgaacca      600

agcaaagccg agaaguuuaa guccaucuug cuggaugaag acgcuacuuc aaaugauaca      660

cagaaagccc uagugaagcu ccugcuuagu gacgauggag acaaagaggu acugaagcag      720

cagaaaaagg uguugaccga guucgcaaaa gcugucgccg gccucaaggu gaaguuuaac      780

guugcgcugg gcauugaagu gacggaugcc gacgucgaga aauggcacuu cgggauggca      840

caauuagaag auaaguggag cgacaucgag cccucucuga cugaucaggg aauggaaaua      900

uuugagcaga uucaggaccu cuaccgcgcu agacuucuga augguaucgu gccugccggc      960

aaaaccuugu cccaagcuaa gguagacgau uacgaccagc auugcaaaga ucuaaagcug     1020

uucaaggagu aucucaaaca gcugaacgac cacgaucuug ccaagaggau ucggcuguug     1080

uacgaccgau auaucgaugg ggacgaugca aaaccauuuc uccgcgacga uuucguuaag     1140

gcccugaaaa aggaagugac agcucacccg acugaguuag cggaaucacu gcuagagaga     1200

auggaacaag acaauuuuau gcucaagcag aggaccaaag caaacggaag cauccccguc     1260

cagaugcagc aacgggagcu ggauaagauu auagccaauc aggcugagua cuauaguugg     1320

cuugccgcag aaaacccugu gaaagagcau cgcgacucua agccauacca guuggacgaa     1380

cugcucaauu ucagaauccc cuauuacgug ggcccuauga uuaccaaaaa gcagcaagag     1440

aaguccgguc agaacgucuu ugccuggaug guuaggaaag auccaagcgg gaacaucaca     1500

cccuacaauu ucgacgaaaa gguggaucgu gaggcuucgg ccaacgcauu uauacagcgg     1560

augaaaacga cugacacaua ucugauugga gaggauguac uuccgaagca gucucuguug     1620

uaccaaaaau augaagugcu caaugagcug aacaaugucc gagugaacgg cgaauccuua     1680

ucaaccgacc agaagaaaag acugaucagg gagcuauucg agcgccacag uagcguuacu     1740

cucaagcagu ucguggaaaa ucuggucgcg cauggcgaug uggcuaagcg gccugacgua     1800

agagggcuug ccgaugagaa aagguuuuug uccucucuga gcacauacca ccagcuccgc     1860

ggaauccugc augacgcaau ugaugaccga gguaagcaau cagauaucga aaacauuaua     1920

accuggagua cgguuuucga ggacgccucc aucuuuaaaa gcaaguuagg cgaaauuucu     1980

uggcuggacu caaaaacccg gacaacucuu aguaauaucc guuauagagg guggggacag     2040

uucagcagga aguugcucga uggccugaag cuagguaacg ggcacaccau cauucaggag     2100

cugaugcucu ccucucgcaa ucugaugcaa auacuuacag acgagacuuu gaaaaaggaa     2160

auggcuaccc ugaaccagga gcgguuaaaa cccgauucga ucaaugaagc cauugacgca     2220

gcuuacacaa gcccauccaa caagaaagcc aucagacagg ugcucaaggu cguggaugac     2280

auuaagcacg cggccaauga ucaggcuccu ucauggcugu auaucgagac cgcagacggc     2340

ggaggccauc ccgggaaaag gacucuuagu cgacaaaagc agauacaggc caucuacgaa     2400

aacacggcua aagagcugau ugauucugcc guccgcggag aguugaagga caaaaucgca     2460

gauaagacaa acuuuagcga ccggcucgug cuguacuuca ugcagggugg ccacgacauu     2520

uauaccggga cugcgcuaaa uaucgaucaa cuguccucau acgacauaga ucauauucuc     2580

ccacagagcc ugaucaagga cgauucucuu gacaacagag uuuuggugca cgccaaaauu     2640

aauagggaaa agaacgcuga guuugccucc acccuguucg cagcuaaaau ggccaguaca     2700

uggcaccguu ggcgcgaugc aggauuaauc agcggccgga agcucaaaaa ucugcuuaug     2760

agacccgacg aaguagauaa guaugccaaa gguuuugucg cuaggcagcu gguggagacc     2820

cgacagauaa ucaagcuaac ugaacaaauu guugcggccc aguacccuga cacaaagauc     2880

auugcaguga aagcugggcu cucucaucag cugcggcaag aguuggauuu cccaaagaac     2940

cgcgacguca aucacuauca ucacgccuuu gaugccuucc uggcugcaag aaucggcacg     3000

uacuuacuca aaagguaucc gaaccuggcc cccuuuuuca ccuacggaga guucacuaag     3060

acaaaucuua aaaagcugca guccuuuaac uucauaggcg cguugaccca uacagacggg     3120

aagauuacuu caaaagacac cggagaagug cucugggaua aucagcggga caucgaggaa     3180

cuggaucaga ucuacaacuu uaagcgaaug cuaauuacgc acgagguaua uuucgagacc     3240

gcuggucugu uuaaacaaac aguguacgcc gcaaaggaca guaaagaaag aggcgggagc     3300

cgccagcuua ucccuaagaa gaaaggauau ucgacuggca uuuacggugg guauacccag     3360

gagacaggcu ccuacaaugc ccucguuagg cugacuaagg cuggaaaagu cguguaucgu     3420

guguucaaga uaucuaccca gaacgcagcc gaaaucgcgg cugcccggag cuugucagca     3480

gaggaaggca augagagacu gugucaaauu uuagagcaga gucuccugaa aacgaacaag     3540

gauaagaaaa acacagucga ccuuuuugau guugugaucc caaggcuggg gaaggaagcc     3600

uuguuccaca auuccaaaua cggacucuuu cuggugaacu cugacaccua cuaucauaau     3660

uaccaggagc uauggcugag ccgcgaaaac cagaagauuc uuaaaaagcu cuuuuccacu     3720

aaguaugauc aaucagagau ggaccgagau gcucugcacu uguuugaaac aaucgccaaa     3780

cagguagagc aguucuucaa guuauacgac auaaaucagu uuagaaccaa aaucagcagu     3840

gcauucgaug cuuuugagaa gcugcccguc gaaggcgagg aagguaaagu ggagacucuc     3900

aagcaacugc uuauugggcu gcaugccaac ccuguucggu cugacuugaa aaaucuagga     3960

aucaagaccc cauucggcuu ucugcaggcg gguaagggca uucaccucga cgaugacaca     4020

gaaaucauac agcaaucccc cgccgggcug uucaaaaggc gcgugcgucu ugcagauuua     4080

uga                                                                   4083


<210>  3061
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3061
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uugguagcaa cuccauaggc       60

uucgccgcug ucgaugaaaa uucucaccuc aucagacuua aggggaaaac aguuauugga      120

gcaaggcugu uugaagaggg caaggccgcg gcugaccggc gcgccucacg aacuacgcgu      180

agaagguuga gucggaaccg cuggagacug agcuuccuga gggacuuuuu cgaaucccau      240

aucaccccua cagguccaaa cuuuuucaug cggcagaaau auucugagau uucgccgaag      300

gauaaggcac gcuacaaaua ugaaaagcga cuauuuaaug auagaacuga cgccgaguuc      360

uaccaacagu aucccaccau guaccaccuc aggaaucggc ugcuuacaga uccuucaaaa      420

gcugacgugc gugagaucua uuuugcaaua caucacaucu uaaagagccg cgggcauuuc      480

uugaccagug gauacgccaa agauuuuaac acuaauaagg uagcucucaa cgaaauuuuc      540

ccagcccugc aggacgcgua cgcacaggug uaucccgauc uggacaucac auuugaugag      600

aauaagguca acgaguucaa aaccgugcuc uuaaaugaga aggccacgcc uuccgacacu      660

caaagagcuc ugguuaaccu uuugcuggcc gaggacggcg auaaagacau ucuaaagcag      720

cagaaacagg ugcucaccga guucgcaaag gcugucgugg gccugaaaac aaaacuuaau      780

guagcccugg ggacugaggu ugauucuagc gaagcgaccg uguggaacuu uuccuuggga      840

caacucgacg auaagugggc cgguaucgag ucagcaauga cagacgaagg caccgagaua      900

cuggaucaga uuagggacuu auaccgggcu cgacugcuca augggaucgu cccagccgga      960

aaaacgcuua gucaggcuaa gguggaugac uaugcccagc accacgagga ucuggaauug     1020

uucaaagacu accuaaagca acuggaggac gauggcacug caaaagccau ucgccagcuc     1080

uaugacagau acaucgaugg ugacgaugcu aagcccuuug ugagggaaga cuucgucaag     1140

gcgcugacaa aagagguuac cgcacauccu aacgccaaga gcccagaacu ucuggagcgg     1200

uuggcucagc cggauuuuau gcucaaacaa cgcaacaagg ccaauggggc aauccccgug     1260

cagaugcagc agagagagcu ggaccaaauu auaaagaacc agucuguaua uuacgauugg     1320

uuagccgcuc cuaauccagu ggaaaagcac aggaagucca ugcccuacca gcuggacgag     1380

cuacucaacu uccguauccc uuauuacguc ggaccacugg ugacugccaa agaacagaag     1440

gcagcgcaag gcggcguuuu ugcuuggaug gugcggaaag aucccgacgg gaauauuaca     1500

ccguauaacu ucgaugagaa ggucgaccga gaagccucag caaauaccuu uauccagaga     1560

augaaaacga ccgacacaua ccuuauagga gaggaugugu ugccuaagca gagccugcuc     1620

uaucagaggu acgagguacu gaacgaacuu aauaacguuc gcgugaauaa cgagaagcug     1680

aguauugaac aaaaacagca ggucauccgg gaguuguucg agagacauaa uucugugacu     1740

aucaagcagu uugccgaaaa ccucagggcu cacggugacu augcccacau ucccgagauc     1800

cgcggccugg cagaugaaaa acgauucuua uccagccugu cgaccuaccg gcaacuaaag     1860

ucucuccugc cagacgcuau ugaugacccu gccaaacagg cggauauaga gaauaucauu     1920

gccuggucca cagucuuuga agacgcugca aucuucaaga cuaaacuuaa ggagaucaac     1980

ugguuggauu cacagaccau uacaaagcug aguaacauac guuacagagg guggggacaa     2040

uucagccaua aauuucucaa uggccugacc uuagguaacg ggcacacuau cauucaggag     2100

cuguuccuuu ccaauaacaa uuugaugcag auccucacgg acgaaacacu gcagaagaaa     2160

augaccgagc uaaacgccga uaagcugaaa gcugccaaua uuaacgacgc aaucgaugac     2220

gcguauacuu cucccagcaa uaagaaggcc cucaggcaag ugcugcgcgu uauagaagac     2280

aucaaacggg cugccgaugg ccaggaccca ucauggcuuu acguggagac cgcagaugga     2340

ggcgggacac ccggaaagag aaccagggcu cgacagcauc aguugcaaga aauuuaugcc     2400

aacgcagccc acgagcugau cgacagugcu guacgcggug aguuagaaga uaaaauugcg     2460

gacaaggccg auuuuaauga ccggcucguc cuguacuuca ugcagggcgg gagagauauc     2520

uauacuggaa caccucuuaa cauagaccag cuguccagcu acgauauuga ccauaucuug     2580

ccacagucuc ucauuaaaga caauucacug gauaacaggg ugcuaguuca cgcaaucaua     2640

aaucgugaga agaacgcuac guuugccagu accaucuaug cccaaaagau gaacgcuacu     2700

uggcgccagu ggcacgaagc aggccugauu agcggucgga aacucagaaa ucugcagaug     2760

aggccggacc aaaucgauaa guacgccucc ggguucgugg cgcgacagcu ugucgagaca     2820

cggcagauua ucaaauugac cgaacagaua guggcugccc aauaccccga gacaaagauu     2880

aucgcaguaa aagccggccu gucucaucag uuacgcgagg aacucgaguu uccuaagaac     2940

agagacguga aucacuauca ucacgcuuuc gaugcauuuc uggccgcgag gaucggaacu     3000

uaccuucuga agcgguuccc aaaccuacag gccuuuuuca ccuauggcaa auuuaagaaa     3060

gcugacguua agaaacuccg aggguucaau uucauuagag auaucacgca cgcagaagac     3120

aagauugucg ugaaagauac cggagaggug auaugggaca agaagcgcga ugucgacgag     3180

cuggauagga ucuacaacuu uaaacguaug uugauuacac augaaguuca cuucgagaag     3240

gccgaccugu uuaaacagac uguguauggu gcuaaggacu cgaaagaagc cggcgggaag     3300

aagcaauuaa ucagcaaaaa gaaaggauac cccguggaua uuuauggcgg uuacacccag     3360

gagacagggu ccuaccucuc aguaguccgg cugacuaaga aagcaaugua ugcugugguu     3420

aaggugagua ccagagacgc cgcgaaacuu gccgucgcaa aaucuaucag cgaacagaag     3480

gagaaugaga cgcugaacga uauaaucgac gaaaaauugu ccaagauuuc aaagaauggc     3540

aagaucacac augaacucuu cgaagugguu cugccuaggg ugggacagaa gacccuauuu     3600

aaaaacagca aguacaaucu guuccuugua aacucugaua cuuauaugca caauuaccaa     3660

gagcucugga ugccacgcga auaucagaaa auguggaagg acauuucccu gaguaaauac     3720

ggcgaugcuc agacagagga ccaguuggau caaaucuuug gguucauagu cagccaggug     3780

aacucuuauu uuaaucugua cgacauuaac caguuccgaa agaaguuaaa cgaugccgcu     3840

gacaaauuug caacccuccc caugagagau acugacgaca ugcaaggaaa gaucgccacc     3900

aucggccagc ugcuuauugg ucugcaggcc aaugcugcgc gguccgauuu gaggaaccuc     3960

gacauaaaaa caccgcuggg gcuacugcag aucccuaaug gaauuucacu ugauagugac     4020

acgagcgugg ucuaccaauc gccaaccggc cuccuggagc gcgaaguuca uuuguccgau     4080

uuauga                                                                4086


<210>  3062
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3062
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg auggucuguc       60

guuacagaug acuauaaggu gccugccaaa aagaugaaag uacucgguaa uacugauaag      120

gaguacauaa agaaaaaccu uaucggcgcu cuguuguucg acucagggga aacggcagag      180

gccaccagaa ugaagaggac agcgcggcgc cgauauacuc guagaaggaa ucggauuuua      240

uaccugcagg auaucuuuag uccagaacua aaccaagugg acgagagcuu ccuccaccgc      300

cuggaugacu ccuuucuugu cgcugaagau aaaagaggag agaggcaugu gauuuucggc      360

aauaucgccg acgagguuaa guaucacaaa gaauuuccga ccauauacca ucugcggaag      420

cacuuggcag auucuucgga gaaggccgac cuccgccugg uguaucuggc ucucgcacau      480

aucauuaaau accgagguca cuucuuaauc gaugaaccca uugacaucag aaacaugaac      540

ucacagaauc uguuuaagga guuccuuuug gccuuugacg ggauacaggu cgauugcuac      600

cuggcuagca aacacacaga cauuagugga aucauuaccg ccaagaucuc caaaucuagg      660

aagguggagg cgguacuaga acaguucccu gaucaaaaga agaauagcuu uuucggcaac      720

augguuuccc ucguguuugg ccugaugcca aauuucaagu caaacuucga gcuugacgaa      780

gaugcaaaac ugcaguuuag ucgggacagc uaugaugagg acuuggaaaa ucuccugggg      840

aucauuggag augaguacgc cgacgucuuc guggcugcca agaaagugua ugauucuaua      900

uuacuguccg guauccucac uacaaacaau cauucaacca aggcaccccu uagcgcuagu      960

augauugacc guuacgacga gcacaacucu gauaaaaagc uguugcgcga cuuuaucaga     1020

acgaacauag gcaaggaagu cuucaaagag guguuuuaug auacuuccaa gaauggguac     1080

gccggauaua uugacggcaa aaccaaccag gaagauuucu acaaguaccu aaagaaucug     1140

cuccagaagg uugacggugg ggauuauuuu aucgagaaga ucgaaaggga ggacuuccug     1200

cggaaacaac gaacauuuga uaacggaagc auuccucauc agguacaccu ugacgagaug     1260

aaggcgaucc ugcgcagaca gggcgaguuc uacccauuuu ugaaagaaaa ugccgaaaag     1320

auucagcaaa uacucacuuu caaaaucccc uauuacgugg gcccucuggc aagggggaac     1380

ucgcgguuug cuugggccuc uuauaauucc aacgagaaga ugaccccaug gaauuucgau     1440

aacgucauug acaagacauc aagugcucag gccuucaucg aacgcaugac caauaacgau     1500

uuauaccugc cggaccagaa agugcuaccc aagcacagcc uccuguauca aaaauuugca     1560

guuuacaaug agcuuacgaa gaucaaauac gugacugaga caggagaagc cagauuguuc     1620

gacgucuuuc ugaagaaaga gauuuucgau ggucucuuua agaaggaaag gaaagugacc     1680

aagaaaaaga uacugaacuu ccuugacaaa aauuuugaug aguuccguau cacugacauu     1740

cagggccugg auaacgagac agggaacuuu aaugcuuccu auggaaccua ccaugacuug     1800

cucaagauca uuggcgauaa ggaguucaug gacucuagcg auaacguaga cguucuggaa     1860

gauaucgugu uaucacugac gcuauuugaa gaccgggaga ugauaaaaca gcgacuccug     1920

aaguaugaag auaucuucag uaaaaagguc auugcgaauc uuaccagaag gcacuacaca     1980

gguugggggc gcuuguccgc aaaacugauc aacggcauua aggacaagca uagccggaaa     2040

acuauccucg acuaucugau agaugacgga cacucuaaua gaaacuuuau gcaguuaauu     2100

aaugaugaca accugucauu caaggaugag aucgccaaua gucaagugau uggcgacggg     2160

gaugaccuuc aucaggucgu gcaggaguug gcuggaagcc cugccaucaa aaaggguaua     2220

cuccaguccc ugaaaaucgu ugaugagcua gugaagguaa ugggcuacaa cccagaacaa     2280

auugucgugg aaauggcaag ggagaaucag accacagccc gcgggcgaaa caauucucag     2340

cagcggcugg gaucgcucac uaaggcuauc caagacuucg gcagcgauau ucugaaacgu     2400

uaucccguug aaaacaauca gcuucagaac gaccaguugu accuguacua uuuacaaaac     2460

gguaaggaca uguacaccgg ggauacacuc gacauccaca aucuguccca guaugauaua     2520

gaccacauua ucccucaguc auuuaucaaa gauaacaguc uugacaauag agugcugacc     2580

aacucuaaga gcaauagggg caaauccgau aacgucccau caaaugaggu gguaaagcgc     2640

augaaaggau ucugguugaa gcaacucgac gccaagcuga uuagccagcg gaaauuugau     2700

aaccuaacua aggcagagag aggcgggcug ucugcggaag acaaagcugg auucaucaag     2760

aggcagcucg uggagacgcg acagauuaca aaacauguug cccgcauacu ggaugaacgg     2820

uuuaauagag acuucgauaa gaacgacaag aggauccgua augucaaaau ugugacccuu     2880

aaguccaacu uggugaguaa uuuucgcaaa gaguucgguu uuuacaaggu ccgggaaauc     2940

aacaauuucc accaugcaca cgacgccuau cugaacgcug uuguggccaa agcauuacuc     3000

auuagauacc ccaagcugga gccggaguuu guguauggcg aauacccuaa guacaacagc     3060

uauagggagc gaaaaucugc uacugaaaag auguucuucu acuccaauau caugaacaug     3120

uuuaaaacca caauaaaacu ugccgauggg cggguagucg agaaacccgu gaucgaggcg     3180

aaugaagaga ccggagaaau ugccugggac aagacuaagc acuucgcuaa cguuaaaaag     3240

gugcugucau auccacaagu caguaucgug aaaaagguug aggaacagac aggcgguuuu     3300

agcaaagagu cgauucuacc uaaagggggc uccgauaagc ucaucgcacg caaaacgaag     3360

aauaacuacc ugucuaccca gaaauaugga ggcuucgaca gccccacugu ggccuacuca     3420

auaauguuug uagcugauau ugagaagggg aaaaguaaga gauugaaaac agucaaggaa     3480

augaucggaa ucaccauuau ggagaggucc cgguucgaau cuaauagcgu gacauuucug     3540

gaggaaaagg gcuaucgaaa cauaagagag aauacuauca uuaaauuccc aaaguacucc     3600

uuauuugagc ucgaaaacgg ucgcaggcgu cugcuugccu cagcaaucga gcugcagaaa     3660

gggaaugaaa uguucuugcc ccaacaguuu gugaaccucc uguaccaugc gcagcacgcc     3720

aauaaggagg acagcgucau uuaucuagaa aaacaucggc acgagcugag ugagcuuuuc     3780

caucacauca uaggaguuuc ugaaaagacc auccucaagc cuaaagugga gaugacgcug     3840

aacgaggcuu uugaaaagca cuucgaguuc gaugaaguau ccgaguuggc ccagagcuuu     3900

auuucgcugu uaaaauucac cgcauuuggc gcuccaggug gcuucaaguu ucucgacgcc     3960

gauaucaaac aaucaaaucu gagauaccag acagugacug aaguccuuuc uaguacccug     4020

auucaucagu ccgugacagg guuguaugag acuaggaucg accucagcaa gcugggaggc     4080

gaauga                                                                4086


<210>  3063
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3063
augggcaacu acuaucuggg gcucgacauc ggaaccaaua gcguggguug ggccgucaca       60

gaugagaacu acaauauuga aagauucaag aaaaagaaca uguggggcau aaggcuuuuu      120

gacgaggcua aaacugcaga aucccggcgc auccaccgac aggcccguag aaugaggcaa      180

cggaagauuc agcgccugaa uuuguuaaug gagcuguucg auguugaaau cucuaaagug      240

gacgagucau uuuucauuag aaagaaggag agugaucuac auuaugaaga caaaagccag      300

gauuccaggu acgcgcucuu uaacgacaag gaguucaaug auaaagacua ucacaacaag      360

uaccccacga ucuuucaucu gauaauggau cuuaucgaaa augacgagaa aaaggauauu      420

cggcuguugu aucucgcuug ccacuaccug aucaagaacc gcgggcauuu cauuuuugag      480

ggacagaaau ucgacguaaa uucuucgcug aacaauaaca ucaauaagcu caaaucacac      540

uuagaccaau uuuacaacau augucuggau uucgacaacg auaaacuuau ugaaaucuug      600

gugaaucaga accugaauau uagcgagaaa aguaagaaaa ucaaggaacu acuccgaaag      660

accaaccugg aggucggcau cgccaaucuu auugccggug gguccauaaa acugucuaac      720

auguuugacg augacgaauu gaagaaucuc gauuauaaca gccuguccuu caaaaauuca      780

aacuucgacg agggaagaga guuauuugaa gaggugcugg aagauagguu cgagcucguu      840

gaaauccuua aggacauuca cgaugcaagu gugcuggaga caauguugag cucugcuucc      900

aucucaccug acggcggcaa auacauaagc aguucuauga uuaauuccua uaacaagcau      960

aagcgggacc uaaaacugcu caagaaaauc aucaagaaau acaaaaagag cgaguaugau     1020

aauauuuuuc gugacgaaaa caucaaaaau aacuaccugu cguauacucg caccgggauu     1080

aacaagaaua aaaaggucca ggcagaggau aacacagacu cugauggauu cuacaaauac     1140

auagugaaga uccuuaagga cauuaaagaa aaugauuccg acgaggauau caaguauauc     1200

cugucagaaa uugacaaaaa gaacuuugua ccaaaacaga gaaccaagga gaauaguguu     1260

auaccguacc aauugaggga gaucgaacuc cggaaaauua ucgauaacca gagcaaguau     1320

cacaaguucc ugaaugaggu ggacgaaaaa gguauuuccg ucaaggagaa aaucauaucu     1380

uuacugacuu uucgaauccc cuacuauauu ggcccucuaa acccaagcua caaugaggaa     1440

aagaacuuca caaccucaug ggugaaacgc cucgccaagg ggaaggugac gcccuggaau     1500

uuugaggaua uggucgacau cgacgcuagu agagaagccu ucauugagaa caaacugaau     1560

aagugcuccu uucuuuugga ugaaacuguu cugccuaaag acagccuccu guaucaggag     1620

uacauggugc uuaacgagcu gaauaacuug aagaucaacg gacagucuau aaauaccgau     1680

cucaaaaagu caauuuacga agaacuguuc aagaaauaua agaaaguaac aaggaagcgg     1740

uuaaucaguu acauuaaaga aaaggagaac augagcggcg acaucgugau aacugguauc     1800

gauggggacu uuaagcaagg acugaaaucc uauauugcgu ucaaggauau cauuggcgaa     1860

aagaucgacg augacauaua ccgcaaggag gucgagaaaa uuaucagaga uaucucucua     1920

uauggcgacg auaagucguu cauucagaaa aagaucaaga augaaaacaa acucgaccug     1980

agcgacgauc agauuuccaa gauagcaaaa cuuaaguuua aagacugggg gagguucuca     2040

aagaaguugc uggugggaau caaagccagu gauaagaaua ccggugagau ugacaacauc     2100

auuggcuuua ugcguaauua caacuauaau aucaugcaac ucauggcuaa cguuuucaca     2160

uuuaaagaug aaauagagaa gcugaauagu aaauacuaca ccgacgaaaa gaucaacauu     2220

gauuauuuag acgaucugua ccuuagcucc ccagccaagc ggauguugug gcagucauuc     2280

cagaucgugg acgagauuaa aaauaucaag aacagcgauc ccaaaaagau auuuauugag     2340

auggcacgau cuaaagaaga caagccuacg agaaagaugu ccaggaaaag ucgccucgag     2400

aagcuguaua cugacagcaa aaagcaguuc aucaaacuau uugggccaga ugaauacgac     2460

aagcugaaua acgagaucaa gaacaaaucu gaguccgauu uccgguggga caaucucuau     2520

cuguacuaua cacaauuugg aagauguaug uacucacuug auccgauuga cuuggaggag     2580

cugaguaaca agaauuuaua cgaucaggac cauauauauc ccaaaagcaa gaucuacgau     2640

gacucgauug aaaacagggu ccucgugaaa aagcagcuga aucuugauaa auccaacgag     2700

uauccuaucc cagaccgcgu acugaauaag gauaagaaca aauugcucgc uuucuggaag     2760

augcugaaug aaaaagagcu aauuggcgac aagaaauacg cccgacugau ccggaagucu     2820

cccuuuaccg acgaggaacu cggugauuuc auagcgcguc agcugguuga gacuagacaa     2880

gccacaaagg aaaccgcaaa caugcuuaaa agguugugcc cugacagcaa gaucguguau     2940

gucaaagcua agaauguguc agauuuccgc cggaaauuug acauucugaa guguagaagu     3000

aucaacgauu uacaccacgc ccaugacgcu uaccucaaua uugucguggg gaacguuuuc     3060

gauacgaagu uuaccuccaa uccacugaac uucaucaaaa auaacucuaa gaacaggagc     3120

uauaaucuug agaaaauaua cgacuacgau gugauucgaa acgaaaagau cgccuggcug     3180

gcagagcgca aagaggaaaa ucaggacggc uccaucgcca caguacuaaa gaaccucuca     3240

aagaaugaua uucgggucac uagaaggacc uauaucaaau gcgaggaccu guuugaugaa     3300

aacauagugc guaagggaaa uggcaaauuc cccauuaagg agaacaauaa caaaagcgac     3360

acaaauaagu acgggggaua uagugguauc aaaaacgcuu acuuuguuuu gcuggaagac     3420

gagaagcaca aucgcauuuu agaggcgauc ccgucuuuca uauauaagaa agaaaagaac     3480

ggcgagaaag gguccgugau uaauuaccuc cagaacaucc ugggaaucaa gagcgcaaaa     3540

auucuucggg auaagaucaa gauuggcucg cuguugaaaa uagacaaguu uccuuauaac     3600

cucgccggua aaucagggaa uggcauccug auuucuggag gcguccagcu aauccuggau     3660

aagaacacuu cuaaauacau caagaagauu gaaaauuaca aauauaaacu ugacgagaac     3720

aaggauuacc aaauaaauau cuucgacaag cucuccaaag aggaaaacau uaagcuguuu     3780

gauuuguucg uggacaaguu cgagaauagc aucuauaaaa acagaguaaa uauuaagcug     3840

aaagaaauca aguuagcuag gcccaaauuu gagcaucucu cuauagagga acagaucaag     3900

acccugacac agaugcuuau ucuguucggg cgagagaaca augccgugaa cuuggaauac     3960

aucggacucc caaagaauac cgguaagacu aagauuaaca aaaagaucaa uaacaaagau     4020

auaaagauua ucaaccagag cauuacgggc cuguaugaga cagaagagga ccuagcaaau     4080

aucuga                                                                4086


<210>  3064
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3064
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccauaggu       60

uucgccgugg ucgaugcuaa cucucaccuu cugagauuga aaggcgagac agcaaucggg      120

gccagguuau uucgggaagg acaaucagcg gcugagcgcc gagaaagucg uacuacgaga      180

aggcggcuga gccgcaccag auggaggcua uccuuccucc gggacuuuuu cgccccucau      240

auuacaaaga ucgauccaga cuuuuuccug cgccagaaau auucugagau uucgccgaag      300

gauaaagacc gauuuaagua cgaaaagaga cuuuucaaug auaggacuga cgcagaguuu      360

uaugaggauu accccucaau guaucaucug cgguugcacc ucaugaccca cacacauaaa      420

gccgacccuc gugaaaucuu ccuggcuaua caccacaucc uaaagagccg cggccauuuu      480

cucaccccag gugcagccaa agauuucaac acugacaagg uugauuuaga ggacauuuuu      540

cccgcucuga cagaagccua cgcacaggug uauccugacc uugaguugac cuucgaucug      600

gcgaaagccg acgauuucaa ggcuaagcua cucgacgagc aggccacgcc aagugauacu      660

caaaaagcac ugguaaaucu ucuguugucc ucugacgggg aaaaggagau cgugaaaaag      720

agaaaacagg uccucaccga auuugcuaag gccauuacag gacugaaaac uaaguucaac      780

uuagcgcugg gcaccgaggu ggaugaagcc gacgcaagca auuggcaguu uuccaugggc      840

cagcucgaug acaagugguc aaacaucgag acaaguauga ccgaucaagg gacggagaua      900

uucgaacaga uucaggagcu uuacagggcu cggcuguuga auggaaucgu ucccgccggu      960

augagccuau cucaggcuaa aguggccgac uacggccaac acaaggaaga ccuggagcuc     1020

uuuaaaacuu aucugaagaa acuuaacgau caugaacugg caaagacaau ucgaggguug     1080

uacgaccgcu auaucaaugg agaugacgcc aagccuuucc ucagagagga uuuugucaaa     1140

gcucugacca aggaggugac ugcgcaccca aacgaaguau ccgagcaguu acugaauagg     1200

augggccagg caaacuucau gcuaaaacaa cggacaaagg ccaauggugc uaucccgguu     1260

cagcuccagc agcgcgaacu ggaccaaauu auagccaacc agucaaaaua cuaugauugg     1320

cuugcaaagc ccaacccugu ggagaagcac agagccagca aaccauacca guuggacgaa     1380

cugcucaccu uuaggauccc cuauuacguc gggccucuga uuacgccaaa ggagcaggcu     1440

gagagucgug aaaauguguu cgccuggaug auccggaaag aucccucugg aaacauaacc     1500

ccguacaauu uugacgagaa gguggauugc ccugcauccg cgaacauguu cauucaacga     1560

augaaaaaua cagacacuua ucuuaucggc gaagaugucc ugcccaagag gagcuugcuc     1620

uaccagaagu augagguucu gaacgaguua aauaacgugu cgaucgacgg caggcgccug     1680

ggggcugacc agaaacagcg gcuaauuaga gaaguauuug agaggcauuc uuccgugacc     1740

aucaagcaag ucgccgaaaa ucucguggca cacggagauu ucgccaaacg cccagagauu     1800

cgaggucugg cugacgaaaa gcgguuccuu ucaaguuuga gcacauacca ucagcugaaa     1860

aagauacucc acgaggccau cgaugacccu acuaaagagc augauauuga aaacaucauc     1920

accuggucca caguuuuuga ggacgcagcu auuuucaaga ccgaacuggc caagauaucu     1980

ugguuagaua gcgacacuau caaagcgcug ucaaauauuc guuauagagg cugggggcag     2040

uuuaguagga aacuuuugga uggacucaaa cugggcaacg gucacacggu gauccaggag     2100

cuaaugcugu ccaaucacaa ccucaugcaa auucugacag acgaaacccu uaagacuacc     2160

auggccgagu ugaaucagga uaaacugaag gcugacgaua ucgaggacgu cauaaacgac     2220

gcauacacaa gccccucuaa caagaaagcc uuacgccagg ugcuccgggu aguugaagau     2280

aucaagcaug cugccaaugg gcaagaccca ucauggcugu auauugagac cgcagauggc     2340

cccggaacug cgggcaaaag aacacagagu aggcagaagc agauccaaac gguguacgcc     2400

aacgcugccc aggaacuuau ugacagcgca guccgagggg agcuggaaga uaaaaucgcu     2460

gacaaggccu ccuucaccga ucgcuuggug cucuacuuua ugcagggagg ucgggacaua     2520

uauacuggcg caccucugaa uauugaucag cuaucucacu acgacaucga ucauauucug     2580

ccacaaucgc ucaucaagga cgacagccug gauaacagag uccuugugaa ugccacaaua     2640

aacagggaga aaaauaacgu uuucgcuucc accuuguuug cgucaaagau ggccgcaaca     2700

uggcguaaau ggcacgaggc ugggcugauc aguggacgca aguuacggaa ucucaugcug     2760

agaccggacg aaauugauaa auucgcccac ggcuuugugg ccaggcagcu uguagagacu     2820

cgacagauca uuaagcugac cgaacagauc gcugcagccc aauaucccaa cacgaagaua     2880

auugcgguca aagcuggucu aucucaucag cuccgggagg agcuggacuu cccuaagaau     2940

cgcgauguga accacuacca ucacgccuuu gacgcauucu uggccgcuag aaucgggacc     3000

uaucuguuaa aaagguaccc aaagcucgca cccuuuuuca cauauggcga auuugccaaa     3060

guugauguga agaaauuucg ggaguucaau uuuaucggag cgcugacuca ugcuaagaag     3120

aacauugucg ccaaagacac cggcgaaauc gugugggaua aggagcgaga cauucaggaa     3180

cuugauagaa uauacaauuu caaacgcaug cugaucacac acgagguaua cuuugagacu     3240

gcagacuugu ucaagcaaac cauuuaugcc gcuaaagaua gcaaggaaag agggggaucc     3300

aagcagcuca ucccuaaaaa gcaggguuac ccaacacagg uguauggcgg guacacgcgu     3360

gagaacaccg ccuaucuggc aguugucaaa acuguggaaa agaaagcuga cguguaccgg     3420

gucguuagaa uugccacauc acaaguggcg gcccuaaagg aggcaagggc ucagagcacc     3480

gugaaggaac gcgagcugcu uaaaaaguuu cucacuacca aauucacaaa gaucggaaag     3540

aacggcaaga aggccauaac gcccuuugac aucguaauuc cgcgaguccc uagagagcag     3600

cuguuccaca auaaacggua ugguuucuuc auggugaacu cugauaccuu gaugcauaau     3660

uaccaggaac uguggauguc caggagugac caacagaucu uaagcaagcu cguuaaaucu     3720

gcuaagacug uggagaacgg gcaggucggc cgccuguucg aaaauauugu ggagcagauc     3780

aacaaauacu uuccacuuua ugauauaaau caauuccgua agaagcugga ccaguccaaa     3840

gauaucuuuu caaaguugcc ccucgaagga aacggcacaa ccagugggaa aagagagaca     3900

auucagaaua uccugacugg agcaggcacc ggugccaaga acggggaccu gaaaaaucug     3960

ggaauuaaga cgccuuucgg cuuucuucag guucccagcg guauagugcu cgaugacgau     4020

acaacccuga ucuaccaauc gccaacuggc auguugaacc ggauuguaag guuauccgag     4080

cuguga                                                                4086


<210>  3065
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3065
augaagaacg aggaaaauau cuaccugggc cucgacauug ggaccgauag cgugggauau       60

gccgucacag acgauaaaua caagauaaug aaauaucacg guaacgcugc auggggcucc      120

acuaucuucg acgccggggc gcuuggagcu gauagaaggg uuuuucgguc ugcccgccga      180

cgucuggaca gaaggcagga gcggauucaa uuguuacagg aaaucuucgc aaaugagauu      240

gccaaggugg augaacgcuu uuucaucaga cugcuagagu caggccucug gagggcugac      300

gcagaggauc gguacguauu uuucaacgac aaagaauaua cggaugugca guacaugcgc      360

gacuauccca ccauacauca ccugaucugc gagcuuauuu acaauccuaa caagcaugau      420

guccgacugg uguacuuggc cugugcuugg cucguuacac acagagguca uuuucugagu      480

aauaucaaca cugaaaauau ugccgaccug guggacaucc acaacgucua uaauggguuc      540

augagguuuu ucgaggagaa cgaaauaauu ugcgauuggc gggaggugaa uguagaggaa      600

cucggacgug uuuuagcggc aaacaucggc auuaaugaaa aguaccgcaa ccugagcacc      660

gugcuuuuug ccacaagaaa accagacaag aaagcuaagg agggcuuccc guauuccgag      720

gaugccaucg ucagguugcu ggcaggggga accuguaaac uaaaggacgu guacaucaag      780

gaagaguaug augaauuugg uucuauuacu cucggcaugg acgaugagaa aaugacagaa      840

auaauggcug acaucgggga ugacuucgau cugauuaagg cccuucgguc gcuguacgac      900

uggggaaucu uggcgaacau acucgugaau gagaacaauu caccccugau uagccagagu      960

aaagucaagg uuuaugagca acaccgagaa gacuuagcca accugaaaua ccucgugcau     1020

aaguacaucc cucacaaaua uaaugagauc uuccgcgaaa uuagaggcga uaacuacgua     1080

gcauauuccu accauuauga gcuuaagaau gacgaaaaga uggguaaaaa ggcugaugug     1140

gagaaauuuu cuaaguuccu guugaaaauc cuagagggga uuagcgucga cgaacaggau     1200

aaggugguuu acgacaacau gauagagagg cugacccucc ggcuguuucu uccaaagcag     1260

aaaaauacgg auaaccgcgu gaucccccac cagcuguaug aauacgaguu gaaguccauu     1320

cucaaaaaua cuaagaacua ccugccuuuc uuaaaagaga aggacucaga uggaaucagu     1380

aaugaagaca agaucauuag cauauuuaaa uucaagaucc cauauuuugu cggcccccug     1440

aacugcaauu cucaauucgc cuggauucag agaaaagcug auggcaagau cuuuccuugg     1500

aacuucgagg acaaagugga uuuugacgcc uccgaacagg cauucauuca gaggaugacc     1560

aaccaaugua cauaccuacc agaggaauca guacucccga agcagagccu guauuaccag     1620

aaguuuaugg uucuuaauga gaucaacaau auaaaaaucg acgggcguaa gauuagugug     1680

gccaugaaac aagaguugua uaacgaaauc uucgagaaga aaaagcgggu ccgaaagaag     1740

gaugugauug acuucuuugu cucuaaugga uaccuggaag guggcaagga ggcucucauc     1800

uccgggcugg augaacagau acacacuaac cuuagcucgu uccaugcguu uagaaggcug     1860

uugcagaauc acauucucuc uguggaggac guugagucca ucauugaacg cgcaucauau     1920

agugaugaca aacggagagu gcuguuauuc cugaagaaca aauacagcuu ucuaaccgau     1980

gaggacauca aguacauaug caaaaucaag auuaaggauu ucggaaggcu cuccaaaaag     2040

uuucugguag acuucgaagg cacauguaaa gagaccggug aaaucucuac gguccuucgc     2100

uugauguggg agacuaauaa caaucugaug gagcuccuga gcgauaagua uacauuugcc     2160

aacgggauua aagaaaaucg aaagaaguac uauaccgagc agcaaaagac uuuagacgau     2220

cggcuggacg aaauguacau cucaaacagu gugaagcgua caauauauag aacccuuucc     2280

guugugaaag acgucacgaa ggcuuucggc gugcccaaaa agauuuuugu agagauggcc     2340

aggaccaaua acccugauuu gaagggaaaa cgcacacgga cuagacagca acaggugauc     2400

gagcucuacg caaagugcga cgaagaggau guuagggaac ugaaacauca aauggagagc     2460

cuaggcgaau augucgacaa caagcugcag ucugauaaac ucuuccugua cuuuauccag     2520

uucgggaagu cagccuacag uggaaaaaug auugaccuug agaaguugau gagcgguucc     2580

aagguguaug auaucgagca cauuuaccca caggcuuaug ugaaagacga uucuauaauc     2640

aauaacaagg uccugguuuu cucggaagag aauggcgcca aacaagacau uuaccccauc     2700

aagaccgaaa uucgaaaaca gaugagcggg aucuggacaa cuuggcauca cgugggagca     2760

auauccgaug agaaguauaa gcgcuuaacc cggucaacac cuuuuaacga agacgagaaa     2820

uacggcuuca ucaauagaca gcucaccgag acuucccagu cuacgaaggc gauugcucaa     2880

auccugaacg aaaaauaucc agagacagaa auuguguacg uaaaggccgg ucuugucagc     2940

gacuuuaggc acgaguucgg gcuguguaaa ugccguuccu acaaugauuu gcaucacgca     3000

aucgacgccu aucucaacgu gguugugggc aaugucuaca acaugaaguu uaaucgcaag     3060

ugguucucag uggauagcaa auauucuaua aagaccaaaa cuauuuuuag ucauagucug     3120

guuuguaacg gaaagaucau cugggacaaa gaaaagaugc uacccauggu gcugaagaau     3180

gcuaaaaaga acaaugccca cuucaccaaa uaccaguuua ugaagacagg cggguucuuu     3240

gaucagaugc cgugcaaaaa ggcaagcgga cucguaccuc ggaagaaagg ucuggacacc     3300

gagagauaug gcggguacaa caagucugga auuauguucc uuacucccau gagguauaaa     3360

auaggcaaga aauccgauau cauuaucuug ucaauugagc uguuauacgg uaaacucuuc     3420

cugaaagacg aagucuuugc uaagaaguac gccgaggaac gacuucagaa aaucauaggg     3480

aagaaagcgg augaggugag uuucccaaug ggcaugaagc cuuggaaaau caacacagug     3540

cugagccuag acggauuuaa ugucugcauu acgggcaucg ggggaggcgg uaagugucuc     3600

ucggcccagc ccauuaugca gcuguccuug ccauaugagc uggcuaagua ccucaaaaag     3660

aucgaaaaau ucguugagaa gaacaaaaau aacgcaaauu auauauacga ugaacaguau     3720

gacucuguga gcguagauga gaacauuaag uuauacgacg uguaucugga caaacuugaa     3780

aauucaaucu acaacaaacg gaucaauagu cccgaggcca uucugaagaa cgggcgcgag     3840

aaauuuugca aguugaaugu ccucgaacaa gcugugaccc ugaucaacau acaguccauu     3900

uucggaagaa ugacuggcgg uugugaucua gagaauaucg gcgggaaaaa gcacucugcc     3960

gcaacaguua acuuuagcuc caauguguca aacuggaaga aauacuauaa ugacgucagg     4020

gugguuuacc agagcccuag uggaauuugg gaacggcgau cucagaacau ccuggagcuu     4080

cucuga                                                                4086


<210>  3066
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3066
augaacgagc ccuacggcgu ggggcuggac aucggaacca auagcgucgg uuggacaguu       60

guggauauga acggcagagu aaggaaggug aaagggaaga cugcccucgg agcucggcuu      120

uucaaagaag gcgcaacggc cgaggaccgc cgagguuuuc guaccacaag aaggcggcug      180

aagcgcguca aauggagauu gagguuacug cgggaguucu uugaucagcc uauuuccaag      240

auagacccaa auuucuuugc gcgccgaaag uauucugaua ucucaccgag agaccccaac      300

uacaaugggc uagcuaaaac ucucuucaac gauaggaccg acaaggaauu uuaugaugac      360

uacgccacaa uuuaucaucu gcgggauaaa cuuaugacca guaaucguaa guucgacauc      420

cgcgaaauuu accuggcaau ccaccacaua gugaaauaua gaggaaacuu uuugaggacu      480

ggcccugcca gccaauacgg cuccgcuucu cuccaucugg caacaucguu ccagaagcug      540

aaugaucucu uugcccaguc agaggagacc uuaaaccuga aacuuguuac ggacgaagcu      600

cuacugcagc aaauccagca gauucuagug cggacugacc ucagccgaag ugagcagcaa      660

cgccagaucu ggccacugau ggccguccuu accggggcgu ccgcagccga aaagaagaga      720

cagaaaaaug ugguaguuga gcugucuaag gcuuuggugg gacucaaagc caagaugaac      780

gucgugacac ugacugaggu ggaugcagcu gucguuaaag acuggaccuu cacaauggaa      840

gagagccagg auaaguuacc cgaaauugag gaacaacugu ccgagguggg ucagcagauc      900

auggacgagg uaauacaacu cuacgccuca gugaaucuug cgcagcugau uccugccggc      960

aagagguuua gucagcacau ggucgaaaaa uauaagcauc acgagaaaaa cuuggaacua     1020

cugaaggcau acauccacag ccagucugau uccaaacggg ggcgcgagau uagagcuacc     1080

uaugacaggu acaucgaugg aguggacuca aagccaguua cgcaagaaau guucuauaag     1140

gaucucauga aauacgugga ggccgacgcu acuagcaauc aucuggccgc agagaucaag     1200

gaugaaauug acagugagca guucaugccc aaacuucgua caaagcagaa cggcucuaua     1260

ccuuaucagg uccaacagua cgaacuggac cagaucauuu cccaccagaa aaaguacuau     1320

ccaugguugg gugaggaaaa cccgguggcc gagcggcgag ggaaguuucc cuacaaacuc     1380

gaugagcugg uaggauucag aguuccuuau uacgugggcc cauuaaucac caaggaagac     1440

caacaggcua cuagcggcgc gggguuugca uggaugguca ggaaagccga uggacccaua     1500

acaccuugga auuucgacca gaagguggau cgcauugcuu cggccaccgc auuuauccag     1560

cggaugcaaa cgaccgacac auaucugauc ggugaggaug uccuaccagc cagaucucuc     1620

auuuaccaga gguucauggu gcugaacgaa cuuaauaaca ugcgcguuga ggaccgaaaa     1680

uuggcucccc agcaaaagca gcggcuguau aaucaggugu uuaagcagca ucaacacgua     1740

uccgucaaaa acauccagca gaaucucaug gaugccggcg aguaccguaa gacuccgcag     1800

auuaccgggc uggcagaccc uaaaggauuc aacucaaguc uuagcacaua ccacgauuuu     1860

aagaaaauac uguuggaagc gaucgcugac gagcauaaga gagccgauau ugaaaagauc     1920

auccucuggu ccacuaccuu cgaggacucu gcaauuuuua agcaaaagcu ggaagaggug     1980

gccugguuaa cagacgcuca gaggaaacag cugagcggcc uacgcuaucg ggguuggggg     2040

cagcucucac acaagcugcu uaccgccuuc aaagaugaca agggcagaag uauaauggau     2100

ggauuguggg agacuuccga caauuuuaug caacugagga aacagcccgc aaucgaaacg     2160

cagauuaagg aggcuaacca agccaaucuc acagaugcgg acauccagga uaccauuaac     2220

gaacuguaca cuagcccaca gaauaagaaa gccauccgag agguuauguu agugcuggac     2280

gauauaaaga acgcuaugca uggccagacc ccuucuugga ucuucgucga agcagcccgc     2340

gggggaggug uggcuggccg gagaacacaa ucaaggagua gccagauugu agaggccuau     2400

aaagggaccg caaaggagau cguguccgaa aaaguucagc acgagcuuaa ugaaaagauu     2460

aaggcgaaag ccgacuucaa cacucguuug guccucuacu uucugcagaa cggacgcgau     2520

cuauauacaa augaggcuau caacauagac cggcugucug aauacgauau ugaccauauc     2580

cucccccaau cgcuggugaa ggacgauagc cuugacaaua gaguguugac guccgccagg     2640

auuaaccgag agaaaaauga uaccuucgca ucagagaagu uuggccggaa aaugggugcu     2700

caguggcgcg aacugcacag aaacggguua augacucaga ggaagcucaa gcaucugcuu     2760

augcggccag acgagaucag uaaacacgcc acagauuuca uaaaucgaca gcuggucgaa     2820

accagacaag uuaucaaguu gguggaggag cucauuucua gcgaauaucc cgcagccucc     2880

aucguggcug uaaaagcgaa ccugacacac caguuucgcc agacuuucaa uuuuccuaag     2940

cuaagggagg ucaacgacua ccaucacgcc uucgaugcau cacugaccgc uuuuauuggc     3000

auguaccucc ugaaacagua uccaaaacuu gaacguuucu uuguguacgg aaaauucgcc     3060

aagcaaccga ucaauuugac gcgguuuaac auaauuagaa agcuggccgu ugcugagaaa     3120

cccaucgcaa auaucgaaac cggcgagauu uuaugggaca agacagccga uaucaaauau     3180

uucgagaagc ucuacaacua uaaaaggcug cuugugacuc acgaaguccg cgagaauuac     3240

ggggcgaugu ucaagcagac ccuguuuaag gcuagcuaua acaaaucuaa gacauuggug     3300

ccuaaaaaga accauaugga aacuuccguu uacggagguu acaguaauca ggagaccgcc     3360

uaucucgcaa uugugcgaau accauucaaa agcggcuuua aguucaucgu agucgggauu     3420

cccacgagaa ugguggccaa gaucaaacac uaccaaucuc ugggagcuac auugaaccag     3480

gcaacccaua aggugauuga accuaaauuu acuaagaucu cccggaaaac aaagcagacc     3540

gucauaucag acuaugaggu ugugcugcca aagguauacu uagaucaggu ggucagggac     3600

caagugaaag gccagaugua ucgcuucagu cucgguagcg acaaggagua ccacaauguu     3660

caggaacugu aucuuccccu gucgauccag caagccuuug uggggcauua cgaugagucc     3720

gacgaucagc guucuaacga cuuggucaaa guguacgaug cggcucucaa gcagcugcag     3780

agauauuucc cgcuacaccu gagccggaau uuugaccaag uugccucaca ggcacaccag     3840

aguuucgaag gccuuaaaaa caaugugcaa acuuccgaua agcagcucgg aaagaaagag     3900

guccugaacu cuuuguuugu aggccugcau gccaaugcua ccaggagcaa cuuauccgug     3960

cucgggaugu caaaggacuu cggacgcauu aaaagcaaug gcaucacacu gacggaucag     4020

gccgaaauua ucuaccagag uccuaccggu cuuuucgagc gaaagguugc acugaaagac     4080

uuguga                                                                4086


<210>  3067
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3067
augggcaacu acuaucuggg gcucgacauc ggaaccaaua gcguggguug ggccgucaca       60

gaugagaacu acaauauuga aagauucaag aaaaagaaca uguggggcau aaggcuuuuu      120

gacgaggcua aaacugcaga aucccggcgc auccaccgac aggcccguag aaugaggcaa      180

cggaagauuc agcgccugaa uuuguuaaug gagcuguucg auguugaaau cucuaaagug      240

gacgagucau uuuucauuag aaagaaggag agugaucuac auuaugaaga caaaagccag      300

gauuccaggu acgcgcucuu uaacgacaag gaguucaaug auaaagacua ucacaacaag      360

uaccccacga ucuuucaucu gauaauggau cuuaucgaaa augacgagaa aaaggauauu      420

cggcuguugu aucucgcuug ccacuaccug aucaagaacc gcgggcauuu cauuuuugag      480

ggacagaaau ucgacguaaa uucuucgcug aacaauaaca ucaauaagcu caaaucacac      540

uuagaccaau uuuacaacau augucuggau uucgacaacg auaaacuuau ugaaaucuug      600

gugaaucaga accugaauau uagcgagaaa aguaagaaaa ucaaggaacu acuccgaaag      660

accaaccugg aggucggcau cgccaaucuu auugccggug gguccauaaa acugucuaac      720

auguuugacg augacgaauu gaagaauuuc gauuauaaca gccucuccuu caaaaauuca      780

aacuuugacg agggaagaga gcuguucgaa gagguguuag aagauagguu ugagcugguu      840

gaaauccuca aggacauuca cgaugcaagu gugcuugaga caaugcugag cucugcuucc      900

aucucaccug acggcggcaa auacauaagc aguucuauga uuaauuccua uaacaagcau      960

aagcgggacu ugaaacuacu gaagaaaauc aucaagaaau acaagaagag ugaguaugau     1020

aauauuuucc gugacgaaaa caucaaaaau aacuaccucu cguauacucg caccgggauu     1080

aacaagaaua aaaaggucca ggcaaaagau aacacagacu cugauggauu uuacaaguac     1140

auagugaaga uccugaaaga cauuaaggag aaugauuccg acgaagauau caaauauauc     1200

cuuucagaga uugacaagaa aaacuucgua ccaaagcaga gaaccaaaga aaauaguguu     1260

auaccguacc aacugaggga gaucgaguug cggaagauua ucgauaacca gagcaaguau     1320

cacaaauuuc ucaaugaagu ggacgagaag gguauuuccg ucaaagaaaa gaucauagcc     1380

cuguuaacuu uccgaauccc cuacuauauu ggcccucuga acccaucuua caaugaggag     1440

aaaaacuuua caaccagcug ggugaagcgc cuagcuaagg ggaaagugac gcccuggaau     1500

uucgaagaua uggucgacau cgacgccuca agagaggcgu uuauugaaaa caagcucaau     1560

aaaugcaguu uccugcuuga ugagacuguu uugccuaagg acucccugcu cuaucaggaa     1620

uacauggugc ugaacgagcu uaauaaccug aaaaucaacg gacagagcau aaauaccgau     1680

uugaagaagu cuauuuacga ggaacucuuu aaaaaguaua aaaagguaac aaggaaacgg     1740

cugaucucau acauuaagga gaaggaaaac acuagcggcg acaucgugau aaccgguauc     1800

gauggggacu ucaaacaagg auuaaagagc uauauugcau ucaaagagau cauuggcgaa     1860

aagaucgaua augacauaua ccgcaaagag gucgagaaga uuaucagaga uaucucccug     1920

uauggcgacg auaaaucuuu uauucagaag aagaucaaaa acgaaaauaa gcuagaccuc     1980

ucggauaacc agauuagcaa aauagccaag cugaaauuca aggacugggg gagguuuucc     2040

aagaaacuuu uggugggaau caaggcuuca gacaaaaaua caggugagau ugauaacauc     2100

auuggcuuca ugcguaauua caacuauaau aucaugcaac ugauggccaa cguuuuuacc     2160

uucaaggacg aaauagagaa acucaauagu aaguacuaca cguaugaaaa gaucaacgug     2220

gauuaccugg acgauuuaua ucugucugca ccagcuaaac ggaugcuuug gcagagcuuu     2280

cagauugucg acgagaucaa gaauauuaaa aacuccgccc ccaagaaaau cuucauagag     2340

auggcgcgau caaaggaaga uaagccuacu agaaaaauga gcaggaaguc ucgcuuggag     2400

aaacucuaca cagacuccaa gaaacaguuu auuaagcugu ucgggccaga ugaauaugac     2460

aagcuaaaca augagaucaa aaacaagagu gagagcgauu uucgguggga caaucuguac     2520

cucuauuaca cccaauucgg aagauguaug uacucucugg acccgaucga ucuugaagag     2580

uuguccaaca aaaaucugua ugaccaggau cauauuuacc ccaagucaaa aauauaugac     2640

gauaguaucg aaaacagggu guuaguaaag aaacagcuca aucuggacaa gagcaacgag     2700

uacccuauuc cagaucgcgu ucuuaauaag gacaaaaaca agcuguuggc cuuuuggaaa     2760

augcucaaug aaaaggagcu gaucggcgau aaaaaguaug cacgacuaau ucggaagucg     2820

cccuucacug acgaggaacu gggugauuuc aucgcucguc agcucgugga gacaagacaa     2880

gccaccaaag aaacggcuaa caugcugagg cgccuuugcc cugacuccaa gauagucuac     2940

gugaaagcca agaaugucuc ugauuuucgg agaaaauucg acaucuugaa guguaggagc     3000

auuaacgacc ugcaccacgc acaugaugcc uauuuaaaua ucgugguugg gaacguguuu     3060

gacaccaagu ucacaucaaa uccacucaac uuuauuaaaa acaauaguaa gaaccgaucc     3120

uacaaucugg agaaaaucua cgauuaugac guaauacgca acgaaaagau ugcuuggcuu     3180

gcggagcgga aagaggaaaa ucaggauggc ucuaucgcaa cuguccugaa gaaccuaagc     3240

aagaaugaca ucagagugac caggcguaca uacauuaaau gcgaggaucu cuucgacgaa     3300

aacaucguuc gcaagggaaa uggcaaauuu ccccugaagg agaacaauaa caaauccgau     3360

acuaauaagu augggggaua cucagguaua aagaacgccu auuucguguu gcuggaagac     3420

gagaagcaca aucggauuau cgaggcuauu ccgagcuuua ucuacaagaa agaaaagaac     3480

ggcgagaaag ggagugucau aaacuauuua cagaauauuc ucggaaucaa gucugccaaa     3540

auccugagag auaagauuaa gaucggcucc cuucugaaaa uugacaaguu cccuuacaac     3600

uuggcaggua aaagcgggaa uggcauacuc aucucgggag gcgugcagcu gauucuagau     3660

aagaacaccu caaaauacau caagaagauc gaaaauuaua aauacaagcu ggacgagaac     3720

aaagacuauc aaauuaauau auuugauaaa cuuucuaagg aggaaaacau caaacucuuc     3780

gaccuguucg uagauaaguu ugagaauagu auuuacaaga acagggugaa uaucaaguug     3840

aaagaaauua agcuggcccg auccaaauuc gagcauuuaa gcaucgagga acagauaaag     3900

acacucaccc agaugcugau ccuuuuuggg cgggagaaca augcuguuaa ccuggaauau     3960

auuggauugc ccaaaaauac ugguaagacg aagaucaaca aaaagauuaa caauaaagac     4020

aucaagauaa uuaaccaguc uaucacaggc cucuacgaga ccgaagagga ucuggccaau     4080

auuuga                                                                4086


<210>  3068
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3068
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

auaacagagg auuauaaggu uccugcuaaa aagaugaaag ugcucgguaa uacuucuaga      120

gacuucauca agaagaaccu ucugggcgca uuguuauuug auucagggga aacggccgag      180

gcgaggcggc ugaaacgcac cgcucgacgu agauacacaa ggcggcgcaa uagacuaagg      240

uaucuccagg aaauuuuccu ggacgagaug aacaaacuug auaguggauu uuuccaccgg      300

cuggacgaua gcuuuuuggu accagaagag aaacgcggcu cccgauaccc gaucuucggu      360

acucuccaag aggaagugaa uuaucaugag aacuuuccca ccauuuacca ccugagaaag      420

aaacuggccg acucuaagga aaaggcagau cucagguuaa ucuaucuggc ccuugcucau      480

augauaaaau accgggggca cuucuuguac gaggagucgu uugacucaaa uaacaauagc      540

auccaggaaa uuuucaacga guuuacagca gaauaucaua auaccaucga gggaaguugc      600

cuguccggcc agauugaaca ggucgaggcc auccuaacug auaagauauc uaaaagcgcu      660

aagcgugagc gcauucucaa acuguucccu gccgaaaagu ccacaggccu uuucucagag      720

uuccugaaau ugaucguggg gaaccaagcg gacuucaaga agcacuuuga ucucgaagag      780

aaaaccccac ugcaguucag uaaggacacg uacgaagagg acuuagagaa ucugcucgaa      840

cagauuggag augaguuugc agaccuuuuc cuguuggcca aaaagcuaua ugaugcuacu      900

cugcucgccg guauccugac cgcaacagac guuagcacua aagcuccccu uucugccucc      960

augaucaaca gauacgaaac ccaccagcaa gaucuggcgg ccuugaagca guuuauuaag     1020

aauaaacucc cugagaagua uucagaagug uucagcgacc ugaguaaaga uggcuacgca     1080

ggguauauaa acggaaagac aacccaggag gcuuuuuaca aauacaucaa gaacuuacug     1140

auuaaguucg agggcgccgu cgacuuucua gaaaagaucg agagggaaga uuuccuccgg     1200

aagcagcgaa cguuugacaa ugguucuaua ccacaucaac ugcaccuuca ggagaugaac     1260

gcuauuuugc gcagacaggg ggaucauuau cccuuccuga aagagaauag ggaaaagauc     1320

gagaaaaucc ucacuuuucg gauuccuuac uaugugggac cacuggcccg cggcaacaga     1380

gacuucgcau ggcuuacacg uaauuccgac caagccauca ggccguggaa cuucgaagag     1440

guaguugaua agaccagcuc ggcugaagac uuuauuaaua agaugacuaa cuacgaucug     1500

uauuugcccg aggagaaagu gcucccuaag cacucuauac uguacgaaac auucgcgguc     1560

uauaaugagu uaaccaaagu gaaguuuauc gcagaaggcc ugcgggacua ccaguuccua     1620

gauuccgggc agaaaaagca gauuaucaac cuccuguuua aagacaagcg aaaggugacg     1680

gagaaagaua ucauucacua ccuucauacc gucgacggau augaugguau agaauugaag     1740

ggcaucgaca aacaauucaa ugccucacug aguacauacc acgaucuccu gaagauuauc     1800

aaagacaagg auuuuaugga cgacgcugau aacgaggcca uuuuagagaa uaucguucau     1860

acucugacca uauucgaaga ccaggagaug aucaagcaga gccuugcaca guaugaugcc     1920

auuuuugaca gaaaagugau gaaggcuuug acaaggcgcc acuacacugg guggggagaa     1980

cucuccgcca aacugaucaa cggcauucgg gauaagcaaa ccgguaaaau gauccuagag     2040

uaucugaagg acgaugggga aauaaauaga aacuucaugc agcucauuaa cgacgauucu     2100

cugagcuuua aggagacaau ccagaaagca cagguaauug gcgagaccga aaauaucaag     2160

caaauagugc aggagcuucc aggaucaccc gcgaucaaaa agggcauuuu gcagaguauc     2220

aaaauugucg acgaacuggu ggagaucaug ggguacaacc cugaguccau aguuguggaa     2280

auggcuaggg agaaucaguu cacuaaccaa ggacgccgaa auagccagca gcgguuaaag     2340

aagcucacgg auucuauuaa caaacugggu ucaaguaucc uugaggaaca uccagucgac     2400

aauagccaac ugcagaacga ucguuuguuu cucuauuacc ugcagaaugg caaggacaug     2460

uacacagggg aagcccuaga gcuguccaaa cucucuucgu augacgugga ucacaucauu     2520

ccccaggcau ucaucaagga cgauagccug gacaacagag uacuuguuuc cuacgccaaa     2580

aauaggggaa agucagauga cgugccgagu uuggagauug uccaaaaacg caaggcuuuc     2640

uggcggcagc uguuagauuc ucagcucaua agcgaaagaa aguuuaacaa ucugaccaaa     2700

gccgagaggg gcggucuuac ugaagacgau aaggcagggu ucaucaaacg acagcuggug     2760

gagacccgcc aaauuacaaa gcacgucgcu cagauccuag acgcccgguu uaacaccgaa     2820

gugacugaga aaggcaagaa gaaaagagcg guuaagauua ucacacucaa auccaaucug     2880

gugucaaacu ucaggaagga uuuugaguug uauaaaguac gugaaauaaa cgacuaccau     2940

cacgcccaug acgcuuaucu gaaugcaguc gugggaacgg ccuuacucaa gaaguacccu     3000

aaacuggagc ccgaauuugu uuauggcgau uacccaaagu acaacagcua ucgcgagcgg     3060

aaaaccgcua cugaaaaggu guacuuuuau ucuaauauca ugaacuucuu uaaaacaacc     3120

gucaaacuug ccgacggguc cgagaagaag agacaccaga uugagacaaa ugaagagacu     3180

ggagaaaucg ugugggauaa gaccaaacac uucgagauug uaaggaaggu gcugaguuac     3240

ccucagguua acaucgucaa aaagacggag gaacaaaccg ugggucagaa uggcggguug     3300

uuugacaaca auauagugag caagaacaaa gucguugaug caucuaagcu cauucccauc     3360

aaauccggac ugucaccaga gaaguauggc gguuacgcgc gacccacaau cgccuauagu     3420

gugcuaguga uugcugacau agaaaaaggg aaggccaaaa agcugaagcg gaucaaagag     3480

augguaggca uuacuaucca ggauaagaaa aaguucgaag caaauccuau ugcuuaccuu     3540

gaggagugug gauucaaaaa caucaauagc aaccauauaa ucaagcuccc aaaguacucg     3600

cuguuugagu ucgacgaugg ccaacgcaga uugcuggccu ccucuauuga auuacagaaa     3660

gggaaugaac ucauccugcc guaucaccuu accgcacugu uguaccaugc ccagaggauu     3720

aacaagauca gcgagcccau acacaaacag uaugucgaag cucaucaaaa cgaguuugag     3780

gaacuccuga caacuauuau cucacuaagu aagaaauaca uccagaagcc uaauguggag     3840

ucccugcuuc agcaggcguu cgaccaaacc gaugaagaca uuuaucagcu cucugagagc     3900

uuuaucuccc uguugaaguu aauaucauuc ggagccccag gcgcauuuaa auuccugggu     3960

guugauauua gccagaguaa cgugcgguac cagucugucu ccagcugcuu uaaugcuacg     4020

cucauccacc aaucgauuac agggcuguau gaaacccgaa ucgaccuuuc aaagcuggga     4080

gaggacuga                                                             4089


<210>  3069
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3069
augaacgagc ccuacggcgu ggggcuggac aucggaacca auagcgucgg uuggacaguu       60

guggauauga acggcagagu aaggaaggug aaagggaaga cugcccucgg agcucggcuu      120

uucaaagaag gcgcaacggc cgaggaccgc cgagguuuuc guaccacaag aaggcggcug      180

aagcgcguca aauggagauu gagguuacug cgggaguucu uugaucagcc uauuuccaag      240

auagacccaa auuucuuugc gcgccgaaag uauucugaua ucucaccgag agaccccaac      300

uacaaugggc uagcuaaaac ucucuucaac gauaggaccg acaaggaauu uuaugaugac      360

uacgccacaa uuuaucaucu gcgggauaaa cuuaugacca guaaucguaa guucgacauc      420

cgcgaaauuu accuggcaau ccaccacaua gugaaauaua gaggaaacuu uuugaggacu      480

ggcccugcca gccaauacgg cuccgcuucu cuccaucugg caacaucguu ccagaagcug      540

aaugaucucu uugcccaguc agaggagacc uuaaaccuga aacuuguuac ggacgaagcc      600

uugcugcagc aaauccagca gauucuagug cggacugacc ucagccgaag ugagcagcaa      660

cgccagaucu ggccacugau ggccguccuu accggggcgu ccgcagccga aaagaagaga      720

cagaaaaaug ugguaguuga gcugucuaag gcuuuggugg gacucaaagc caagaugaac      780

gucgugacac ugacugaggu ggaugcagcu gucguuaaag acuggaccuu cacaauggaa      840

gagagccagg auaaguuacc cgaaauugag gaacaacugu ccgagguggg ucagcagauc      900

auggacgagg uaauacaacu cuacgccuca gugaaucuug cgcagcugau uccugccggc      960

aagagguuua gucagcacau ggucgaaaaa uauaagcauc acgagaaaaa cuuggaacua     1020

cugaagaccu acauccacag ccagucugau uccaaacggg ggcgcgagau uagagcaacg     1080

uaugacaggu acaucgaugg aguggacuca aagccaguua cucaagaaau guucuauaag     1140

gaucucauga aauacgugga ggcugacgcc acaagcaauc aucuggcugc cgagaucaag     1200

gaugaaauug acagugagca guucaugccc aaacuucgua ccaagcagaa cggcucuaua     1260

ccuuaucagg uccaacagua cgaacuggac cagaucauuu cccaccagaa aaaguacuau     1320

ccaugguugg gugaggaaaa cccgguggca gagcggcgag ggaaguuucc cuacaaacuc     1380

gaugagcugg uaggauucag aguuccuuau uacgugggcc cauuaaucac uaaggaagac     1440

caacaggcca caagcggcgc uggguuugcg uggaugguca ggaaagcaga uggacccaua     1500

accccuugga auuucgacca gaagguggau cgcauugccu cggcuacggc cuuuauccag     1560

cggaugcaaa ccacagacac uuaucugauc ggugaggaug uccuaccagc aagaucucuc     1620

auuuaccaga gguucauggu gcugaacgaa cuuaauaaca ugcgcguuga ggaccgaaaa     1680

uuggcucccc agcaaaagca gcggcuguau aaucaggugu uuaagcagca ucaacacgua     1740

uccgucaaaa acauccagca gaaucucaug gaugcuggcg aguaccguga agccccgaag     1800

auuaccgggc uggcagaccc uaaaggauuc aacucaaguc uuagcacaua ccacgauuuu     1860

aagaaaauac ugcccgaggc gaucgcugac gaacauaaga gagccgauau ugagaaaauc     1920

aucuuguggu ccacuaccuu cgaagacucu gcaauuuuua agcagaagcu cgaggaggug     1980

gccuggcuga cagacgcuca aaggaaacag uuaagcggcc ugcgcuaucg ggguuggggg     2040

cagcuaucac acaagcuccu gauagccuuc aaagaugaca auggcagaag uaucauggau     2100

ggacuuuggg aaaccuccga caacuuuaug caguugagga agcaaccagc aauugagacu     2160

cagaucaaag aagcuaauca ggccaaccug acggaugcgg acauucaaga uacaaucaau     2220

gagcucuaca ccagcccuca gaacaagaag gccauacgag aaguuaugcu gguguuagac     2280

gauaucaaaa augcuaugca uggccagacu cccucuugga uuuucgucga ggcagcccgc     2340

gggggaggug uggcuggccg gagaacccag ucaaggagua gccaaaucgu agaggccuau     2400

aaggggacag caaaagaaau uguguccgag aagguucagc acgaacugaa cgagaaaauc     2460

aaggcgaagg ccgacuucaa cacccgucuu gucuuguacu uucuccagaa uggacgcgau     2520

cuguauacua acgaagcuau aaauauugac cggcuaucug aguacgauau cgaccauauu     2580

cugccacagu cgcucgugaa agacgauagc cuggacaaca gagugcuuac auccgccagg     2640

aucaaucgag agaagaacga uacguucgca ucagaaaaau uuggccggaa gaugggugcu     2700

caauggcgcg aguugcacag aaaugggcug augacccaga ggaaauuaaa gcaccuccug     2760

augcggcccg acgaaauaag uaagcaugcc acugauuuca ucaaccgaca gcuugucgag     2820

acaagacagg uuauuaaacu gguggaggaa uugaucucua gcgaguaucc ugcagccucc     2880

auuguggcug uaaaggcgaa ucucacccac caauuucgcc agacauucaa cuuuccaaaa     2940

cugagggaag ucaaugacua ccaucacgcc uucgaugcau cacuaacugc uuuuaucggc     3000

auguaccugc ucaagcagua uccgaaacug gagcguuucu uuguguacgg aaaguucgcc     3060

aaacagccca uaaaccuuac ccgguuuaau auuaucagaa aguuggccgu ugcugaaaag     3120

ccuaucgcaa acauugagac gggcgagauc cugugggaca aaaccgccga uauuaaguau     3180

uucgaaaaau uauacaauua uaagaggcuc cuggugacac acgagguccg cgaaaacuac     3240

ggggcgaugu ucaaacaaac ucuuuuuaag gcuagcgaca acaagucuaa aacccuggug     3300

ccaaagaaaa aucauaugga gacauccguu uauggagguu acaguaacca ggaaacugcc     3360

uaccuagcaa uagugcgaau ccccuucaag agcggcuuua aauucauugu agucgggauc     3420

ccuaccagaa ugguggccaa gauuaagcac uaucagucuc ucggagcuac gcugaaacaa     3480

gcaacacaua aggugaucga gccaaaauuu accaagauau cccggaaaac uaagcagaca     3540

gucaucucag auuacgaggu uguguugccc aagguauauc uggaccaggu ggucagggac     3600

caggugaaag gccaaaugua ccgcuucagu uuagguagcg auaaggaaua ucacaauguu     3660

caggagcucu accugccgcu uucgauucag caggccuuug uggggcauua ugacgaaucc     3720

gaugaccaac guucuaacga ucuggucaaa guguacgacg cggcuuugaa gcagcuccag     3780

agauacuucc cucugcaccu aagccggaau uuugaucagg uugccaacca agcacaccag     3840

ucauucgagg gccugaaaaa uaacgugcag aagagugaca agcaacuugg caaaaaggaa     3900

guccucaauu cccuguuugu aggauugcau gccaccgcua cuaggucuaa ccugagcgug     3960

uuagggaugu ccaaagauuu cggacgccuc cagucacgaa gcggcaucac ccugacagac     4020

caggccgaga uuaucuauca gaguccaacg ggucuuuucg agagaaaggu ugcacugaaa     4080

gauuuguga                                                             4089


<210>  3070
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3070
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uugguagcaa cuccauaggc       60

uucgccgcug ucgaugaaaa uucucaccuc aucagacuua aggggaaaac aguuauugga      120

gcaaggcugu uugaagaggg caaggccgcg gcuaaacggc gcgccggucg aacuacgcgu      180

agaagguugu cacggaaccg cuggagauua aguuuccuga gggacuuuuu cgaaagccau      240

aucaccccua cagauccaaa cuuuuucaug cggcagaagu auuccgagau uucuccgaag      300

gacaaagcac gcuacaagua ugaaaaacga cuauuuaaug auagaacuga cgccgaguuc      360

uaccaacagu auucgaccau guaccaccuc aggaaucggc ugaugacaga ucccucacgu      420

gcugacgugc gcgagaucua uuuugcaaua caucacaucc uuaagagcag agggcauuuc      480

cugaccccug gagaugccaa agacuuuaac acuaaugaug uagcuuugga cgaaauuuuc      540

ccagcccucc aggaugcgua cgcacaggug uaccccgacc ugggcaucac auuugacgag      600

gauaaggcca acgaguucaa gaccauucug cucaaugaga ucgcuacgag uauagacacu      660

caaagggccu uagucaaacu gcuuuuggca gaggaagaug acaaggauau ucugaaacag      720

cagaaacagg ugcuaaccga guucgcuaaa gccguugugg gccucaagac aaaacugaac      780

cuugcgcugg ggacugaagu cgacuccucu gaggccaccg cauggaauuu uagcuuggga      840

caacucgaug acaagugggc ugguaucgaa uccgccauga cagaugaggg caccgagauu      900

cuggaccaga uccgggauuu auaucgagcu cgccugcuca acgggaucgu gccuacggga      960

aagacucuuu cacaggccaa aguagacgac uacacacagc accacgaaga ucugcaauug     1020

uucaaggcau aucuaaaaca gcuggaggac gauggcaccg ccaaggcuau uagacagcuc     1080

uacgacaggu auauagaugg ugacgaugcg gcaccauuuc ugcgggaaaa uuucguuaaa     1140

gcccuuacua aggacgugac agcucauccc aacaccaaga guccugagcu guuggaacgc     1200

cucgcccaac cagauuuuau gcugaaacag agaaauaagg caaacggggc caucccgguc     1260

cagaugcagc aaagggaguu agaccagauu aucaaaaacc aggcugugua cuaugauugg     1320

cuggccgcac ccaauccugu ggagaagcac cguaaaagca ugccauacca gcuagacgaa     1380

cuccugaacu uccggauacc cuacuauguc ggaccucuug uuacggcgaa ggagcaaaag     1440

gcugcccagg gcggcguguu ugcauggaug guacgaaaag auccagaagg gaauauuacc     1500

cccuacaacu ucgacgagaa gguggacaga gaagccucug cuaauacauu uauccagagg     1560

augaaaacua ccgauacaua uuugaucgga gaggacgucc ugccgaagca gucccuccug     1620

uaccaacgcu augaggugcu uaacgaacug aauaacguuc gggugaauaa cgagaaauug     1680

ucaauugaac agaagcagca ggucaucaga gagcuuuucg agaggcauaa uagcgugacu     1740

auuaagcaau uugccgaaaa ccugcgcgca cacggugauu acgcucacau accugagauc     1800

cgaggcuuag ccgacgaaaa acgguuccug aguucucuau ccaccuaucg ucagcucaag     1860

agccugcuuc ccgaggcgau ugaugaccca gccaaacagg cugauaucga aaauaucauu     1920

gcauggucga caguauuuga ggacgccgcu auauucaaga ccaaauugaa ggagaucucu     1980

uggcuggggu ccgaagccau uacuaaacuc ucaaacauca gauacagggg auggggccaa     2040

uucagucgca aguuucugaa ugguuuaacg cuggggaacg gccauacaau uauccaggag     2100

cuuuugcuca gcaccaacaa ucugaugcag auacuaacug augaaacccu gcagaagaaa     2160

augacagagc ucaacgcaga caagcugaaa accgcgaaua ucaacgaugc cauugacaau     2220

gcuuacacuu ccccuucuaa caagaaagcc cuucggcaag uuuugagagu ggucgaugac     2280

aucaagaggg cagcugacgg acaggauccc agcuggcugu auguggaaac agccgacggc     2340

gggggaacgc cagguaagcg aacccgcgca cggcagcacc aguuacaaga gauuuacgcc     2400

aaugcugcgc augagcucau cgauacugcc gucagaggcg aacuggagga caaaauauca     2460

gauaaggcag acuucaacga uaggcuugug cuguauuuua ugcaaggggg acgugacauu     2520

uacacaggcg cucccuugaa uaucgaucag cucaguuccu augacauuga ucacauccug     2580

ccucagagcc uaauaaaaga caacucucug gacaaucgcg uucucgugca ugccaucauu     2640

aaccgggaaa agaaugccac cuucgcuuca acaaucuacg cacagaaaau gaacgccacu     2700

uggagacagu ggcacgaggc gggucugauu agugggagga aacuucgaaa uuugcagaug     2760

cggccagauc aaaucgacaa guaugcuagc ggcuuuguag cccgccagcu ggucgaaacc     2820

agacagauaa uuaaguuaac ggagcagauc guggcagccc aauacccgga uaccaagauc     2880

auugcuguua aagcaggacu cucccaccag cugagggagg aacuugaguu ccccaagaac     2940

cgggacguga accauuacca ccaugccuuu gaugcguucc uggcugcccg aaucggcaca     3000

uaucuacuca aaagauuucc uaaucugcag gcauucuuua cuuacgggaa guucaccaag     3060

gccgacguca aaaaguugcg cggauuuaac uucauuaggg auauaacaca cgcugaagac     3120

aaaaucgugg ccaaggauac uggugaggua gugugggaca aacagcguga uguugacgag     3180

cuggaucgga uuuauaauuu caagagaaug uuaaucaccc acgaagucag guuugagacg     3240

gcagaccucu ucaaacaaac aguguacggc gcucgcgacu cuaaggaagc cgggggaucg     3300

aagcagcuga uuccaaagaa gaaaggcuau cccguggaua ucuacggugg guauuuucga     3360

gagaacaccg cguaccuugc cgucguuaag gugacuaaaa agacagaaac cauauucaag     3420

gugguaaaaa ucgcaacuag ccaggucgcu gcccugaaua aggcuagauc ccggucaacc     3480

gcagaggagu ugagugugcu cacagaacug cuaaaaccua aguuuucuaa aguuggcaag     3540

aacggaaaga uuacggacac cccauucgag gugguccugc ccagggugcc gcgcgaacag     3600

cucuuuuaca augccaaaua uggcuucuuu augguuaaca gcgauacuau guuccauaau     3660

uuucaagaga ucuggguguc ccguucagac cagaagauuc uccagcagau cagaaaagcc     3720

aagauagauu acccuaacgu agaccaagau cuggacaauu uguuccacaa ccuggcugau     3780

cagauuguca aauauuucga cuuauacagc aucgcggggu uuaaggaaaa gaucucucag     3840

ucccaaaaua cauucaacga ucucgcagug gacgauaccg accagagugu gggaaaaauu     3900

acagucauaa augagcugcu uaagggcgcc caggcuaacg guaugacugg gagccugaaa     3960

guuuugaaga ucucuacccc auuuggauuc acgcaggaca aauccggcgu gcucacaaag     4020

gauucaagua uuaucuauca aagccccacc ggucuguuug agcggucggu aaggcuaacu     4080

gaccuguga                                                             4089


<210>  3071
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3071
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uugguagcaa cuccauaggc       60

uucgccgcug ucgaugaaaa uucucaccuc aucagacuua aggggaaaac aguuauugga      120

gcaaggcugu uugaagaggg caaggccgcg gcuaaacggc gcgccggucg aacuacgcgu      180

agaagguugu cacggaaccg cuggagauua aguuuccuga gggacuuuuu cgaaagccau      240

aucaccccua cagauccaaa uuucuucaug cggcagaagu auuccgagau uucuccgaag      300

gacaaagcac gcuacaagua ugaaaaacga cuauuuaacg auagaacuga cgccgaguuc      360

uaccaacagu auucgaccau guaccaccuc aggaaucggc ugaugacaga ucccucacgu      420

gcugacgugc gcgagaucua uuuugcaaua caucacaucc uuaagagcag agggcauuuc      480

cugaccccug gagaugccaa agacuuuaac acuaaugaug uagcuuugga cgaaauuuuc      540

ccagcccucc aggaugcgua cgcacaggug uaccccgacc ugggcaucac auuugacgag      600

gauaaggaaa acgaguucaa gaccauucug cucaaugaga ucgccacgag uauagacacu      660

caaagggcuu uagucaaacu gcuuuuggcc gaagaggaug acaaggauau ucugaaacag      720

cagaaacagg ugcuaaccga auuugcaaaa gcuguugugg gccucaagac aaaacugaac      780

cuugcccugg ggacugaggu cgacuccucu gaagcgaccg ccuggaauuu uagcuuggga      840

caacucgaug acaagugggc agguaucgag uccgcuauga cagaugaggg caccgaaauu      900

cuggaccaga uccgggauuu auaucgagcc cgccugcuca acgggaucgu gccuacggga      960

aagacucuuu cacaggcuaa aguagacgac uacacacagc accacgagga ucugcaauug     1020

uucaaggccu aucuaaaaca gcuggaagac gauggcaccg caaaggccau uagacagcuc     1080

uacgacaggu auauagaugg ugacgaugcu gcgccauuuc ugcgggagaa uuucguuaaa     1140

gcacuuacua aggacgugac agcccauccc aacaccaaga guccugaacu guuggagcgc     1200

cucgcucaac cagauuuuau gcugaaacag agaaauaagg ccaacggggc aaucccgguc     1260

cagaugcagc aaagggaguu agaccagauu aucaaaaacc aggccgugua cuaugauugg     1320

cuggcugccc ccaauccugu ggaaaagcac cguaaaagca ugccauacca gcuagacgag     1380

cuccugaacu uccggauacc cuacuauguc ggaccucuug uuacggcaaa ggaacaaaag     1440

gcggcucagg gcggcguguu ugccuggaug guacgaaaag auccagaggg gaauauuacc     1500

cccuacaacu ucgacgaaaa gguggacaga gaggcaucug ccaauacauu uauccagagg     1560

augaaaacua ccgauacaua uuugaucgga gaggacgucc ugccgaagca gucccuccug     1620

uaccaacgcu augaagugcu uaacgagcug aauaacguuc gggugaauaa cgaaaaauug     1680

ucaauugagc agaagcagca ggucaucaga gaacucuucg aaaggcauaa uagcgugacu     1740

auuaagcaau uugcugagaa ccugcgcgcc cacggugauu acgcacacau accugaaauc     1800

cgaggcuuag cugacgagaa acgguuccug aguucucuau ccaccuaucg ucagcucaag     1860

agccugcuuc ccgaagccau ugaugaccca gcgaaacagg ccgauaucga gaauaucauu     1920

gcuuggucga caguauuuga ggacgcagcc auauucaaga ccaaauugaa ggaaaucucu     1980

uggcuggggu ccgaggcuau uacuaaacuc ucaaacauca gauacagggg auggggccaa     2040

uucagucgca aguuucugaa ugguuuaacg cuggggaacg gccauacaau uauccaggaa     2100

cuuuugcuca gcaccaacaa ucugaugcag auacuaacug augagacccu gcagaagaaa     2160

augacagaac ucaacgccga caagcugaaa accgcaaaua ucaacgaugc gauugacaau     2220

gccuacacuu ccccuucuaa caagaaagcc cuucggcaag uuuugagagu ggucgaugac     2280

aucaagaggg cugcagacgg acaggauccc agcuggcugu auguggagac agcugacggc     2340

gggggaacgc cagguaagcg aacccgcgcc cggcagcacc aguuacaaga gauuuacgca     2400

aaugccgcuc augaacucau cgauacugcg gucagaggcg agcuggaaga caaaauauca     2460

gauaaggccg acuucaacga uaggcuugug cuguauuuua ugcaaggggg acgugacauu     2520

uacacaggcg cacccuugaa uaucgaucag cucaguuccu augacauuga ucacauccug     2580

ccucagagcc uaauaaaaga caacucucug gacaaucgcg uucucgugca ugcuaucauu     2640

aaccgggaga agaaugccac cuucgccuca acaaucuacg cucagaaaau gaacgcaacu     2700

uggagacagu ggcacgaagc cggucugauu agugggagga aacuucgaaa uuugcagaug     2760

cggccagauc aaaucgacaa guaugcgagc ggcuuuguag cucgccagcu ggucgagacc     2820

agacagauaa uuaaguuaac ggagcagauc guggccgcac aauacccgga uaccaagauc     2880

auugccguua aagcuggacu cucccaccag cugagggagg agcuugaguu ccccaagaac     2940

cgggacguga accauuacca ccaugcauuu gaugccuucc uggcggcucg aaucggcaca     3000

uaucuacuca aaagauuucc uaaucugcag gccuucuuua cuuacgggaa guucaccaag     3060

gcagacguca aaaaguugcg cggauuuaac uucauuaggg auauaacaca cgccgaggac     3120

aaaaucgugg cuaaggauac uggugaggua gugugggaca aacagcguga uguugacgaa     3180

cuggaucgga uuuauaauuu caagagaaug uuaaucaccc acgaggucag guuugaaacg     3240

gccgaccucu ucaaacaaac aguguacggc gcacgcgacu cuaaggaggc ugggggaucg     3300

aagcagcuga uuccaaagaa gaaaggcuau cccguggaua ucuacggugg guauuuucga     3360

gaaaacaccg ccuaccuugc ggucguuaag gugacuaaaa agacagagac cauauucaag     3420

gugguaaaaa ucgccacuag ccaggucgca gcucugaaua aggccagauc ccggucaacc     3480

gcugaggaau ugagugugcu cacagagcug cuaaaaccua aguuuucuaa aguuggcaag     3540

aacggaaaga uuacggacac cccauucgaa gugguccugc ccagggugcc gcgcgagcag     3600

cucuuuuaca augcaaaaua uggcuucuuu augguuaaca gcgauacuau guuccauaau     3660

uuucaagaaa ucuggguguc ccguucagac cagaagauuc uucagcagau cagaaaagcc     3720

aagauagauu acccuaacgu agaccaagau cuggacaauu uguuccacaa ccuggccgau     3780

cagauuguca aauauuucga cuuauacagc aucgcugggu uuaaggagaa gaucucucag     3840

ucccaaaaua cauucaacga ucucgcggug gacgauaccg accagagugu gggaaaaauu     3900

acagucauaa augagcugcu uaagggcgca caggccaacg guaugacugg gagccugaaa     3960

guuuugaaga ucucuacccc auuuggauuc acgcaggaca aauccggcgu gcucacaaag     4020

gauucaagua uuaucuauca aagccccacc ggucuguuug aacggucggu aaggcuaacu     4080

gaccuguga                                                             4089


<210>  3072
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3072
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uugguagcaa cuccauaggc       60

uucgccgcug ucgaugaaaa uucucaccuc aucagacuua aggggaaaac aguuauugga      120

gcaaggcugu uugaagaggg caaggccgcg gcugaccggc gcgccucacg aacuacgcgu      180

agaagguuga gucggaaccg cuggagauua aguuuccuga gggacuuuuu cgaaucccau      240

aucaccccua cagauccaaa cuuuuucaug cggcagaaau auucugagau uucgccgaag      300

gauaagucac gcuacaaaua ugaaaagcga cuauuuaaug acagaacuga ugcagaguuc      360

uacaaauaug agaauugccc caccauguac caccucagga accggcugau gacagacccu      420

agcaaggccg augugcguga aaucuauuuu gcuauacauc acauccuuaa aagucgcggu      480

cauuuccuga ccccagggga cgcaaaggau uuuaauacua acaagguagc cgugaauaaa      540

auuuuccccg cuuugcaaga cgccuacgcg caggucuacc cugaucucga caucacauuu      600

gacgagaaca aggugaauga guucaaaacc guucugcuga acgagaaggc aacgccaucc      660

gauacucaga gagcccucgu gaauuuacug cuugcuaaag agggagacag ggauauuuug      720

aagcagcaaa aacaggcccu gaccgaguuc gcaaaggcug ucgugggccu aaagacaaaa      780

cucaacguag cccugggcac ugaaguugac ucuagcgaag cgaccgccug gaauuuuucc      840

cuugggcagc uggaugacaa guggacagga aucgagucag caaugaccga ugaagguacg      900

gagauauugg accagauucg ggaucucuau cgagcucgcc uguuaaacgg caucgugccc      960

gccgggaaaa cucugaguca agcuaagguc gacgauuacg cccagcacca cgaggaccuc     1020

gaacuuuuca aagacuaucu gaagcaguug gaggaugacg gaacagcaaa ggccauuaga     1080

cagcuauacg auagguauau cgacggcgau gacgcuaaac cuuuugugcg ggaagauuuc     1140

gugaaggcgc ugaccaaaga ggucacugca cauccaaacg ccaagagccc ggaacuccug     1200

gagcgccuug cucaacccga cuuuaugcug aaacagagaa auaaggccaa cggugcaauc     1260

ccuguucaga ugcaacagag ggaguuggau cagauuauaa agaaucaguc uguguacuau     1320

gacuggcucg ccgcuccaaa ccccguagaa aaacaccgua aguccaugcc uuaccaacug     1380

gaugaguuac ugaauuuccg gaucccauac uauguggggc cccuagucac agccaaagaa     1440

cagaaggcag cgcagggagg cguguuugcu uggaugguuc gaaaagaccc ggauggcaac     1500

auuaccccuu acaauuucga cgagaaggug gacagagaag ccucagcaaa cacguuuauc     1560

cagaggauga agaccacaga uacuuaucuc auaggggagg acguccugcc caaacaaagc     1620

cuuuuguacc agaguuauga ggugcugaau gaacucaaca auguacgcgu uaacgauaau     1680

aagcugucuu ucgaccagaa acagcaagug cuuggagagc uguuugaacg gcauaacacc     1740

gucacaauua agcaguucgu ggauaauuug cucgcccacg gugacuacga uagaauccca     1800

gaggucaggg gccuggcuga cgagaaacgc uuuuuaucca gccugucgac uuauaaccag     1860

cuaaagggga uccuccacga agccauugau gacucugaua aacagaccga caucgagaac     1920

auuauagcau gguccacaau cuucgaagau aaggcuauuu ucaagaccaa acugaaugag     1980

aucucauggc uuaacgccgg aaugaucaau aguuuggcga acauucgaua ccggggcugg     2040

ggucaauuua gccauaagcu gcucaaugaa cugacuuuau ccacggggcg uacaguguuc     2100

gaggagcugc uuauaucuag ccacaacuug augcagaucc ucaccgacga aguucugaaa     2160

cagaagauug ccgagcuaaa ugcucaugaa cugaaagcau caggcaucua cgccgcuauu     2220

gacgaugccu auacuagccc uuccaacaag aaggcacuca gacaagugcu gagcguaauc     2280

gacgauauaa ccaaugcggc cggacaggcu cccaacuggc uuuacaucga gacagccgac     2340

ggcgaugggg ucccaggaaa aaggaccaau ucucgccagc ggaagauuca ggaaaucuau     2400

gcaaacgcug cccaagaguu gauuacuggu ucagugagag gcgagcugga agacaaaauc     2460

gcaaguaggg ccgauuuuac agaccgauua guucucuacu ucaugcaagg gggacgcgau     2520

auauauacgg gcaagccccu gaauauugac aaccuuagcu ccuacgauau cgaccacauu     2580

cugccucagu cuuugaucaa agacaauucg cucgauaacc gggugcuggu ccauccacuu     2640

gugaacagag agaaggacac cauauuugcu agcacuaggu ucgcgaagca gaugaauggu     2700

auguggcguc aguggcacgc cgcagggcug aucuccucac gcaaacuccg gaaccugcag     2760

augagaccgg augaaauuga caaguaugcu aguggcuuug uagccaggca gcuuguggag     2820

acacgacaga ucauuaaauu gaccgaacaa aucguugccg aucaguaccc cgacacaaag     2880

auaauugcug ucaaagcagg acugucuagc caguuacgga aggagcucga uuucccuaag     2940

aaucgcgagg ugaaccacua ccaucacgcc uuugacgcgu uccuggcugc cagaaucggc     3000

acuuaucuuc ugaaaaggua cccaaaucua gaacccuuuu ucaccuaugg gaaguuuaaa     3060

aagacggagg ugaaaaagcu caaauccuuc aacuucaucc gggauaugac ccaugcaaag     3120

gacaagauug ucgccaaaga aacaggagag aucguuuggg auaaugcuuc agacauuaac     3180

gagcuggauc gaauauacaa uuuuaagaga auguugauca cucacgaagu gcgcuucgag     3240

accgcaagcc uguuuaaaca aacauuauau gccgcgaaga acucuaaaaa uagggguggc     3300

ucccgucagc ucauuccuaa gaagaaaggg uaccuggugg acaucuaugg aggcuacacu     3360

caggaaaccg guaguuaccu uagcguaguc cggcugacga agaaagcuau guaugccgug     3420

guuaaggugu cuacaagaga cgcagccaaa uuggcugucg ccaaguccau uucagagcag     3480

aaggaaaacg agacccucaa aaagaucaua gaugggaaac ugaguaagac uagcaaaaag     3540

ggcaagacaa cccaccaacu auucgagauc gugcugccaa ggguuggaca gaaaacucuu     3600

uuuaaaaauu cgaaguacaa ccaguuccuc guaaauuccg acaccuauau gcauaacuac     3660

caggaacugu ggaugccccg cgaguaucaa cgaaugugga aggauauuuu gcugucuaac     3720

cacggcgacg cacaguuaga agggcagcuc gaucagaucu uuaaauucau ugugagccaa     3780

gucaauucau acuuuaaccu guaugacauc aaucaguuca gaaagaagau agcugaugcc     3840

acagacaaau uugcgaagcu accgacgcau gauaccgaca acgcccgggg aaaaauugca     3900

acuaucggcc agcuguugau cggucuccag gcuaaugcca ugaggaguga ucugcgcaac     3960

cuagacauua agacaccuuu cgggcugcuu caaauaggaa ccggcaucac acucgaucug     4020

gacacuucca uuaucuacca gucuccaacc gguuuguucg agcgugaagu gcccuuaacg     4080

gaccuguga                                                             4089


<210>  3073
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3073
augaaccagg agcccuacgc caucggccug gacauuggga ccagcucccu cggaugggcu       60

aaugcagaua uggacgguua uauagccaga gugaagggcc aaacagggcu uggagucagg      120

cuguucgaag agggccagcc ugcggcugau cggcgcucuu uucgaacuac gcguagaagg      180

uugucacggc gcagauggag guuacggcug cuacgcgaaa ucuucgacga gccaauuagu      240

agcaucgauc cgaacuuuuu cgcccgacag aaagcauccu cuauuucgcc caaggacgau      300

agauuugaaa ccacauacca gcuuuucaau gacaaaacug aucaagaguu uuauucaaag      360

uacccuacca ucuaucaccu gaggugggcc cuuaugacag agcaucggaa auucgaccug      420

cgugaaauau acuuggcugu ucaccauauc gugaaguauc gcgguaacuu ucucagaagc      480

gggaauccaa agaguuucaa acccuccgag cuggauuuuu cucagagguu caaggaagua      540

aacggacugu ggcaccucau uuuugacgag guggcaccuc aguuaccaac cgauaaucug      600

gaggaaauca cuaaacuugu cuuggacaca acccggacgc gagccgaccg ccagagagag      660

agcgugaagc ugcuaauggc ugccacugau aaccauaaag acuggaagcc cuaccucacc      720

gaguucacaa aggcgauucu gggccuuaaa acuaagcugu acuugcucac cggcguugau      780

guggcaaaag ccgaccaaaa gacauggacc uucucccugg aagauaucga agaccacagg      840

gcugauuuag agacggccuu ugacgaucgg ucacuggagc ucauagaaaa acuugucgac      900

cugcaggcag cuauucaguu gacugagauc augccuagug ggcaguauuu cagccaaucu      960

augauuuacc gcuaugauca gcacaaggaa caucuaagau ggcugaaaga cuacgccgag     1020

cagcaaacag acgcgaauaa gaggaaggcc gugcgucucg cauaugauca cuacaucgaa     1080

ggagacgcua aagguaagcc agagaucgcc gaugacuuuu auaaagagcu gaaccggauu     1140

cuuaagggcg auaccuccga acuggcuaaa aagauaaauc augagaucgc cuuggaacac     1200

uucaugccga agcagcgaac uaaagagaac gggguaauuc ccuaccagau ccaccagcaa     1260

gaacucgacu caauaauuga uaaucagaag aaauacuauc aguuucuggg agaggacaac     1320

ccuguuguga gccaucacgg cagucagcca uacaagaucg augaguuagu cucuuucaga     1380

gugcccuauu acgugggucc uaugaucaca gcagaccaac agaaaaagac cgccgcuggg     1440

caguuugcgu ggauggucag gaaggauucc ggagacauua cgccauggaa uuucgaugac     1500

aaaguugacc gcaccgcaag cgccacaaac uuuauccagc ggaugaagac uaccgauaca     1560

uaucugauug gcgaagacgu gcuaccccuc caaucgcuga cuuaccagaa auucauggua     1620

cuuaacgagu ugaauaacau aagaaucaau ggcgaacugc ucaccaggga gcagaagcag     1680

cgcacauaua acgauguguu uaaagggcau ggaaccguca augugaaguc ucugauuaag     1740

ucccuugagu gccacucaga aguuacuggu gcuaucaaag uggagggccu ggccgacccg     1800

aagcgauuca acagugcauu gacgacauac cgggauuuua aaaagauccu cccugucgcc     1860

auugaccaac cccagcguca ggcugauaua gaaagaauca uuaauuggag caccaucuuc     1920

gaggacgccg auauuuucaa agcaaagcug caagaaaucg acugguuaac ugauacccag     1980

agggaccgcc uguccacaaa acgguacaga ggguggggaa ggcuaucugc gaagcuccug     2040

cuuaacauaa aggauaccca gggcuugagc aucauggacc agcuguggaa uacucaacau     2100

aacuuuaugc agauugugca cgagccagac uaugcugaga aaaucgaaaa gacaaaugcc     2160

aaaggucuca agcagcagga uauuuucgag acgaucaacg aaauguacac cucaccucaa     2220

aauaaaaagg cacugcgaca gauauuagcc guaguucgcg acauucagaa ggcucaccau     2280

gggcaggccc ccagucggau cuuuauugag gcagcuagag gcgccgaaca aaacccacag     2340

aggacucguc agcgccaaaa acagcuggcg gaucuuuucg ccgaccaggc uaaggagauc     2400

gugaaugaga cagucgcaca ggaauugaac gaugagaccg acaacaaagc ccgguuuaca     2460

gauagacuca ugcuguauuu ccuacaaaau ggacaggaca uguacacugg cgaaaagcug     2520

aacauagaua aacucuccac cuaugacauc gaucacauuc ugccccagag ccuugugaag     2580

gacgacucuu uggauaauag gguccuggug ucaaagguug agaacuuaaa gaagaaugac     2640

cauuuugcua gugaacucua cgcccgaaaa augcagggga cgugggagcu guggcggagc     2700

gcaggacuua ucuccgcgcg caagcugaaa cacuugcuca ugagaccuga ugagauuaac     2760

aaguaugcca ccgguuucau caggcggcaa cugguggaaa cacgacaggu aauaaagcua     2820

gucacugacg ugcugucuaa ugaguacgau ucggacaaaa ccaagauuau cagcguuaaa     2880

gcuggccucu cccaccagcu gagaauggaa cuugauuugc caaaggugcg cgagcugaac     2940

gacuaccauc acgccuuuga ugcauucuua gcugucagga ucgggacaua ucuccugaaa     3000

cguuacccga agcucgagcg guuuuucgug uauggacagu accaaguagc accccagcug     3060

gaccuaagaa gguuuaauuu cauucaugau cucgugucag gcaaacagga aaagaucguu     3120

gccgaggaaa cuggugagau ugucuggaac cgcgaaaagg acgugaguua uaugaaacac     3180

cuguugucuc ugaagcauuu acucgugacc cacgagguca aagaggauca cggggcccug     3240

uucaaucaga cgauauacaa ggcuaaaaag gacaacggca auguuggagg caagaugaaa     3300

cuucugccua caaagagcaa ccgaccaacc gaaaucuaug ggggacauuc cucacaagcg     3360

auugccuacu uggcaaucgu gagagugaau gguaaagagc ccagcuaccg gguaaugggc     3420

gucccuauua ggauccucga ccagcugaag caggcuaaaa agcaagggga auaugccgag     3480

cgccuagccc ugaagcaguu uuucacucca gauuuuacaa aaggaaaggg caaaauagug     3540

ucugacuucg auguugugau cccccacauu ccgcuucucc agccugucau cgacgaaauu     3600

uccggucaga gucaucguuu ugcucugggg accaacacuu acuauagaaa cuugcaacag     3660

cugaucuuaa cccucgcaga ucagaaggug cugauaagca aagagacagg cguugacgag     3720

gaaaagaaac uuguggaggu auacucucac auuugucagc aagucaugaa auucuuucca     3780

cuguaugauc ggaauaaguu cagggaauug cucguggacu cccgcucaca uuuugagcag     3840

cugcccaucg ugaaccgaua cagugaugcc ggaaaacuag aaaucgcggg caagagaggg     3900

auucugcagc agauaauggu cggacuucac gcuaaugcca cgccuggcga ccucaaagag     3960

cuggguguua agacccccuu cgggaaguug caagugccag gaggcaucaa auuaacugag     4020

aacgcaaagc ugauuuauga aagcccuaca ggucucuuua aacgggaggu aaagaucacc     4080

acagaauga                                                             4089


<210>  3074
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3074
augaagaaaa aggccgagaa aaucuaccug ggccucaacg uggggaccaa uagcauugga       60

uaugcuguca cagacgaaca guacagacuu cugaaguuca acgguaauga ugcauggggc      120

uccauaguuu uugacgccgc gucuucaaac gaggauaggc ggaaucaccg cuuggcucga      180

cguaacuuag acagaaggca acagcggauc guguggcugc aggaaauuuu cgccaaagag      240

aucaguaagg uagaugaccg cuuuuucauu agacuaagcg aauccuuuag guggcgggag      300

gaugugcagg accgcuaugu cuucuuuaau gauaaggagu acacugacgu gcaauauaug      360

aaagaauacc ccacgaucca ucaccucaua ugcgagcuga uggauaacaa gaccccucau      420

gacguucgac uuguguaucu ggcaugugcc ugguugauca cacacagagg gcauuuccuc      480

aauaaccuga augucgaaaa acuggaugag auuauggacu uuucuucggu guaccagaac      540

uucaucaauu acuuuacuga gaacggauau aagaggccau ggggcgaaau ugacguauca      600

gcucucagcg agguuuuaaa acagaagacc gguaucacag ugaagaauaa agaacugcag      660

aacauacuuu uggaggggaa gaaaccggaa aagaguggaa ccgaggaguu ccccuuuucc      720

caagauucua uuauccggcu gcuagcaggc ggccagugca aacucaagga cgucuucugu      780

aaagaagagu accuggaucu ugggagcgug ucccugggaa uggacgaaga gaaguucgau      840

gaaauuucag guaguaucgg cgaggacuau gauuugcuca ucgcccugcg uggguuauac      900

gacuggagcg ugcuggcuaa ccuccuuaau ggacacucug agacuauuuc cauggccaag      960

guccagaugu augaacagca cggcaaagau cugcaaauau ugaaguacuu uguucgcaaa     1020

uaugugccug acaaguacaa cgagguauuc agaaaagcga aggcagauaa uuacguggcc     1080

uauacagguc auauggacaa ggacaccgcu ucacagauca aaaagaaagc caaggcagaa     1140

gaguuuagca aauacguccu aaagcugaug aguccaauua agcccgaaaa gacggaucuc     1200

cagucuuaug aggacaugug cgagaggcug aaacuuaacc gguuccugcc uaaacaaaag     1260

aauacugaua accgagugau cccacaccag uuguacgaau augagcucau acagauucug     1320

aaaaaugcuu ccguuuacuu acccuuucug aacgacacca auaacgggau cagcgaagcc     1380

gauaaggugc uaucggucuu ccgcuauaag aucccuuacu acgugggacc acucaaugag     1440

gagucuuccu uugcguggau uucaagaaaa cagggcaaga ucacaccgug gaacuucaaa     1500

gaaaugguag acgaggaugc cagugaaaau gcauuuauua ggcggaugac uaacaagugu     1560

accuaucugc ccacagagga cguucuuccu aaagauagcu ugugcuacca aaaguucaug     1620

gugcugaaug aaauaaacaa ccuccagauc gacggccuga aaauuccagu caaggcuaag     1680

caggggaucu aucaugagcu uuuugagaaa aauaagaaag ugaagcgcaa agauaucgaa     1740

gaguaccuga uuagaaacaa uuauuuggac aaggauucca ccgaacucau aucuggaauc     1800

gacgagcaga uucacagcgg ucugucaagu uacugugccu ucuuuaacuu acugaauaag     1860

aaaauccuau ccgaagacga ugucgagagg auuaucgagc gugcuagcua ugccgaagac     1920

aagucucggg ugauaaaaug gcuccgaaag gaguaccccg gccugucaga agaugacauc     1980

agauacauug caaggaucaa aauucgcgau uucgggcggc uuaguaagac guucuugacu     2040

ggacugaagg gcugcgacgu uagcaaaggu uuugaugggc aagacgugga gaugaucucc     2100

auacuccaga caauguggga aaccaacgau aaucugaugg agauuuuauc ugacaaguau     2160

acuuuccugg aggcccuuga ggcuuuuaca caggaguacu auaccggcaa aaagaaaucg     2220

uugaaggaaa gacucgauga gaugagggua agcaacuccg uccgccgacc uguguaccgg     2280

acgcuggcgg uugugaagga caucgagaaa gcauucggaa agccaaauaa aauuuuuguc     2340

gaaaugaccc guggcgggca ggccgaucaa aagggaaaaa gaaucaaguc aaggaaacag     2400

cagauacuag accuguauga caaguguaag gaugacgugc acgaucucau guggcagcug     2460

gagagucuug gugaauacgc ugacucucgc uugcaaagcg aucggcugua uuuauacuuc     2520

augcaguuug gcaaaugcgc cuacuccggg gcacauaucg gauucgagca gcucauggcc     2580

ggcucaguag aauaugacau ugaucacauc uacccccaaa gcuaugugaa ggacgauucu     2640

cugaacaaua aaguucuugu ccuguccgac uugaacggua caaaggauaa uauuuacccg     2700

gugaguccug agaucagagg gaaaaugcag ggccucugga gcugguggaa ccauguggga     2760

gcuauaucug acgagaagua uaagaggcug auucgauccg cccccuuuac ugacgaagag     2820

aaaaugggcu ucaucaaucg ccagcuaacc gaaacaucac agaguacuaa guuuaucgca     2880

gaucugcuca aagagcgguu cccagacgcg gaagucguuu acgugaaggc ugggcuggug     2940

agcgauuuua gacacgaguu cgaccuuccu aaaucgaggu ccuauaacga uuugcauaac     3000

gccgcagacg ccuuccuguc uguagucgcu ggaaaugugu acgauaugcg uuuuagcaag     3060

aagugguucc gcauugacga gaaauacuca guuaagguga aaaccaucuu uacacacgaa     3120

gucaaguguc ggggugauau uauaugggac ggcaugccca uguuagagaa aguggaagau     3180

accgccagaa agaacacugc acacuucguu aaguaugcug ccuuuaaaac agggggacuc     3240

uucgaccaaa auccagugaa gaaaggcacg ggucugaucc cccuuaaggc ggggcugccu     3300

accgagaaau acggcggaua uaacaaggcc ggcauuaugu uucuaauccc aguaagguac     3360

aauauuggga agaaaaguga aauccucaua cugccggucg aguugaugca uggaaagcac     3420

uuccuggagg auaaaguguu ugcucgaaac uauacuuuca cccgguuaga aaauauccuc     3480

ggcaagaaag ugggugaggu cuccuuuccc auggggaugc gcccuuggaa gauuaacaca     3540

augcugucuu ucgacggauu cagaguuugc cuuaccggca cuagcgguag gggcaaaugc     3600

cugguggcac agccaguaau gcaguuuucc gccgacgaac auuggcgguu cuacgugaag     3660

cgauuggaga gauuugucga gaagaucggg auuaaucccg aucuccugua ugacaaagau     3720

cacgacaagg ugucaaaaga ugaaaaccua aagcuguacg agcuuuacgu ugacaaacuc     3780

cagaauacaa ucuauaagaa gcgcgugaac agcccugucc aaacgcuguu gaaaggaaag     3840

gaagaguuca aaaagcugag uauagaagaa cagugucagg uguuacucaa uauucagucu     3900

guuuuuggca ggaugaccgg gggaugcgau cugacucuua ucggcgguuc cgcucacagc     3960

gccgcaacag ggucgcuguc aaccacaauc ucuaacugga aaaagaagua caguacugug     4020

cguauuauag accaaucccc aagcggaaug ugggagaaau cuagcgaaaa uuugcucgau     4080

uuccuguga                                                             4089


<210>  3075
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3075
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uugguagcaa cuccauaggc       60

uucgccgcug ucgaugaaaa uucucaccuc aucagacuua aggggaaaac aguuauugga      120

gcaaggcugu uugaagaggg caaggccgcg gcugaccggc gcgccucacg aacuacgcgu      180

agaagguuga gucggaaccg cuggagacug agcuuccuga gggauuucuu cgaaucccau      240

aucaccccua cagacccaaa cuuuuucaug cggcagaaau auucugagau uucgccgaag      300

gauaaggcac gcuacaaaua ugaaaagcga cuauuuaaug acagaacuga ugccgaguuc      360

uaccaacagu aucccaccau guaccaccuc aggaaucggc ugcuuacaga cccuucaaaa      420

gcugaugugc gugagaucua uuuugcaaua caucacaucu uaaagagccg cggucauuuc      480

uugaccagug gguacgccaa agacuuuaac acuaauaagg uagcucucaa cgaaauuuuc      540

ccagcccugc aggaugcgua cgcacaggug uaucccgacc uggauaucac auuugacgag      600

aauaagguca acgaauuuaa aaccgugcuc uuagacgaga aggccacgcc uuccgauacu      660

caaagagcuc ugguuaaucu uuugcuggcc gaggacggag auaaagacau ucuaaagcag      720

cagaaacagg ugcucaccga guucgcaaag gcugucgugg gccugaaaau gaaacuuaac      780

guagcccugg gcacagaggu ugauucuagc gaagcgacug ccuggaauuu uuccuugggg      840

caacucgacg auaagugggc aggaaucgag ucagcuauga ccgacgaagg uacagagaua      900

cuggaucaga uuagggacuu auaccgggcc cgacugcuca acggcaucgu gccagcuggg      960

aaaacccuua gucaggccaa ggucgaugac uaugagcagc accacgaaga ccuggaguug     1020

uucaaagauu accuaaagca acuggaagac gauggaaugg caaaagugau ucgccagcuc     1080

uaugacagau acaucgaugg cgacgaugcc aagcccuuug ugagggagga cuucgucaag     1140

gcucugacga aagaaguuac ugcgcauccu aaugcaaaga gcccagagcu ucuggagcgg     1200

uuggugcagc cggauuuuau gcucaaacaa cgcaacaagg ccaacggugc uauccccgua     1260

cagaugcagc agagagaacu ggaccaaauu auaaagaauc agucugugua uuacgauugg     1320

uuagccgcac cuaacccagu cgagaagcac aggcagucca ugcccuacca gcuggacgaa     1380

cuacucaauu uccguauccc uuauuacgug gggccacugg uuacagccaa ggagcaaaaa     1440

gcugcccagg gaggcguguu ugcauggaug guccggaagg aucccgacgg caacauuacc     1500

ccguauaauu ucgacgaaaa aguggaucga gaggcgucag cuaacacuuu uauccagaga     1560

augaagacaa ccgacacgua ccuuauaggg gaggauguau ugccuaaaca gagccugcuc     1620

uaucaaaguu acgaaguucu gaaugagcuu aacaauguga gggucaacga caauaagcug     1680

ucuuucgauc agaagcaaca gguguuggga gaacucuuug agcgccauaa caccgucaca     1740

auuaaacagu ucguggacaa ucuguuagcc cacggugauu augaccggau ccccgagguu     1800

agaggccugg cagaugaaaa gagguuucua uccagccucu cgacuuacaa ccagcugaaa     1860

gggauccuuc acgaggccau ugacgauucu gacaagcaga ccgauaucga aaagauuaua     1920

gcuuggucca caaucuucga ggacaaggcc auuuucaaaa cuaaguugaa ugaaaucuca     1980

uggcugaccg caggaaugau caacagucuc gcuaacauuc gcuaccgagg cuggggucaa     2040

uuuagccaua agcuguuaaa ugagcugaca cuuuccaccg ggcggacugu guucgaggaa     2100

uugcucauau cuagccacaa ccugaugcag auccuaacgg acgagguacu gaaacagaag     2160

auugccgaac ucaaugcgca ugagcugaaa gccucaggca ucuaugcugc aauugaugac     2220

gccuacacaa guccauccaa caagaaagcc cuucgucagg ucuugagcgu gaucgaugac     2280

auaaccaaug cugcaggaca agcgccuaac uggcuguaua ucgaaacugc cgauggcgac     2340

gggguucccg gaaagagaac caauucuagg cagcgcaaga uucaggagau cuacgcuaac     2400

gccgcacagg aguuaauuac agguucagug cggggcgaac ucgaggauaa aaucgcuagu     2460

agagccgacu uuaccgauag gcugguccuu uauuucaugc aagggggacg agacauauac     2520

acuggcgaac cacugaauau ugauaacuug agcuccuaug acaucgacca cauucucccc     2580

cagucucuga ucaaggauaa uucgcuagac aaccgcgugc ugguacaucc ucucgugaau     2640

cgggagaaag auacaauauu ugcaagcacg agauucgcca agcagaugaa cgguaugugg     2700

aggcaguggc acgcugcggg gcugaucucc ucacguaaac uucgcaauuu gcaaaugcgg     2760

ccagacgaaa uugauaagua cgccaguggc uuuguugcaa gacagcuggu cgagaccagg     2820

cagaucauua aguuaacuga gcaaaucgug gcugaccagu acccggaugc caaaauaauu     2880

gccgugaagg cuggacucuc uagccagcug cgaaaagaac uugacuuccc caagaaccgg     2940

gaggucaacc acuaucauca cgcauuugau gccuuccugg cggcucgcau cggcacauac     3000

cuacucaaaa gauauccuaa ucuggaacca uuuuucaccu acgggaaguu uaagaaaaca     3060

gagguuaaga aauugaaguc ccugaaauca uucaacuuca ucagggacau gacucaugcc     3120

aaggauaaaa uuguggcaaa ggagaccgga gaaaucgugu gggacaaugc cagcgauauu     3180

aacgaguuag accggauaua uaauuuuaag cgaaugcuca ucacgcacga aguaagauuc     3240

gagaccgcuu cucuguuuaa acagacacuu uacgcagcca agaacuccaa aaaucgcggu     3300

ggcaguaggc aacugauucc caagaaaaag ggguauuugg ucgacaucua cggaggcuac     3360

acucaggaaa ccgguagcua ucucucugug guucgucuga caaagaaagc gauguacgcu     3420

guggucaagg uguccacucg ggaugccgca aaacuagccg uugcuaaguc aauuagugag     3480

cagaaagaga acgaaacccu gaagaagauc auagacggga aacuuagcaa gacgucgaaa     3540

aagggcaaaa caacccacca acucuucgag aucgugcugc cuagaguagg acagaagacu     3600

uuguuuaaaa auuccaaaua uaaccaguuc cuggucaauu cugauacaua caugcauaac     3660

uaucaggaau uauggaugcc aagggaguac cagcgcaugu ggaaggacau ucuccugagc     3720

aaucacggcg augcccaacu ugaagggcag cuggaccaga ucuuuaaauu cauuguguca     3780

caggugaaca guuauuuuaa uuuguacgau aucaaccaau uccgaaagaa aauagcagac     3840

gcuaccgaua aguuugccaa gcuccccacu caugacaccg auaacgcgag aggaaaaauu     3900

gccacaaucg gccagcugcu aaucggucug caggcaaaug cuaugcgguc cgaccuuagg     3960

aaccucgaua uuaagacgcc guucgggcug uugcaaauag gaaccggcau cacuuuagac     4020

cuggacacau cuauuaucua ccagagcccu accggucucu ucgagcgcga ggucccacug     4080

acagaucuuu ga                                                         4092


<210>  3076
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3076
augagaggca acuaccacau cgggcuggac auuggaacca gcuccauagg uugggccgug       60

aucgaugaca augcuaaggu caugagggca aaaggcaaga cagccauugg ggcgcggcuc      120

uauaacgagg gacagacugc ugccgaucgc cgacuuuucc guacgaccag aaggcggcug      180

aaucgcagaa aauggagguu gaaguuacug gacgaaaucu uugaucccua cauuacaccu      240

guugacgcag ccuucuuugc ucggcuaaaa caaucuaacc ucucaccaaa ggaucagaau      300

cgcgacugga cuggcaguau gcuguucccg gaucgaaccg acagaaaguu ucauaaagau      360

uaucccacaa ucuaccaccu uaggcauaag cugaugaccg aggaccacca guucgauaua      420

cgggaaaucu auuuggcaau ucaucacauc gugaaauacc gugguaacuu ucucaauacu      480

acaccuguaa gcuccuucuc ugugucggac gucaaguuug acgagcaguu cgaucgccug      540

aacgaacugu augccgcugu gucaccagac gagcccuuug ccauuaaugg ggauaacagc      600

aaagcgaucg agacccucuu acugagugac gaaacgaaga aguucgauaa acaaaagcag      660

uccgcaaaac uuauauuguc ugagacugga gacaaggccc uggauaaaga aaauaccaag      720

guugcuacac agauuagcaa agccauccua ggcuacaaga cucagcucga cgugcugcuu      780

aaguacgaug caauuaacuc cucagcuugg aaaaucgagc uggacgauga gaauaucgac      840

gacaccuugc cuggccuccu gagucaauua gaugaaaaca gacaggagau ggucagcauu      900

auacagaauc uguaugccca ggugacacuc aaccaaaucg uaccaaacgg gaugucucuu      960

uccgaaucaa ugauugagaa guacaaugcg cacagggacc aucugaaauu gcuaaagcag     1020

cuggaagaua aaaucgacga ucccaagaag aaaaagggac ucgaggccgc auaugacgcu     1080

uacguuggua aaaacaugaa gguggccaau caggaugucu uuuaugcuag cgugaaaaag     1140

aaccuggacg auagugaccu ugccaagcaa auagcagauc ugauugaggc ccagaaauuc     1200

augccuaagc agcggaccuc ucagaauggc gugaucccac accaauugca ucagcucgaa     1260

cuggaccaga ucauugagaa ccaguccaaa uacuauccgu gguuaacggc ugcgaauccc     1320

aacccuaagc gaauccacca agcaaaauac aagcuggaug agcuagucgc cuuccgcauu     1380

ccauacuaug uugggccccu cgugacuccu gaggaacagg aaaagagcgc uaaucaggua     1440

uuugccugga ugaaaagaaa gcagccagga acaauaaccc ccuggaacuu cgaccaaaaa     1500

guggacagga uggcaucggc caaucgguuu auccgcagaa ugacuacaaa ggauaccuac     1560

cugauuggcg aggacguccu uccggaugcu ucuuugaucu augaaagguu caaagugcug     1620

aacgagcuca aucugguuaa ggccaacuac caccgucuug cacccgcgca gaaacaggcu     1680

gccuaucggg accuguuuga aaaucagaag uccaucucaa cgaagaaauu gcaaaacuac     1740

cucguggauc aggguuauuu caccgcaaag cccaaaauua cagggcugag ugacccagag     1800

cgauuuaaca gcuccuuagg aacuuacaua gauuucaagg aaaucuuugg cgaccagguc     1860

gauaauccug acagacaaga ugacuacgag aaaauuaucg aguggucuac cguguucgaa     1920

gauaggcaga uuuaugaggc caagcugcgc acacuagacu ggcucacuga agaccagauc     1980

caggcucuga agaccaaacg guacaacggc ugggggagac uuagcaagaa auugcugaca     2040

ggaauaguag augagaaugg ugagaggauc auugacguuc ucuggaacac caaucaaaac     2100

uuuaugcaag ugcagucaca ggaaaguuuc gccaagcgca uccaugaggc aaaugcuaaa     2160

cagaugaagg ucacuggcgu ggaagauauu cuggccgacg cguauacguc cccucagaac     2220

aagaaagcca uccgacaggu cauacgggug guugaugaca uucagaaggc uaugggaggg     2280

guggcaccaa caagcguauc uaucgaguuu acccguucag aagaucaaaa ucccagaagg     2340

acucagaguc gcgagagcca guuacaaaac accuucaaag ucgacgccaa ggagauugug     2400

gauuccgacc ugguuacaga acuugagacc gugucuaaau cgaagaaggg cuugacugau     2460

cggcucuacc uguauuucac acagcuaggu aaagacaugu acacggggga cccucugaau     2520

aucgaugaac ucagcaccua ugacauagau cacauccugc cacaggcuuu uauuaaggac     2580

gauucccuug acaacagagu cuugguguca aaagccguaa auaacggcaa gaguaauaac     2640

gugccgguua aacuguucgg aggcaagaug ggaggguuuu ggaaagaguu agcagaucau     2700

aggcucaucu cuaagcgaaa gcugaaacac cuucugacug accccgauac aauugacaag     2760

uacaccaugc gcgguuucau ccggagacag uuggucgaaa caagccaagu gauaaaacuc     2820

guggcgcaga uucugggcga uaaguaccag aacgacgaua cuaaaaucau cgaggucacc     2880

gccaagauga aucaucagau gagggagaac uuuaagcuaa uuaaaaaucg ugaaguuaac     2940

gacuaucacc acgcugugga cgccuaccug acggcauucg uggggcaaua ucucuacaag     3000

cgcuauccua aacugcggcc auacuuugua uauggaaauu ucaagaaauu uaacgaggau     3060

caugucuccu ucagauacuu uaauuuccuu cacgaauuga uggacgagaa caauaagagg     3120

aucguggaac ccgagaccgg cgagguugug cuggaacgag agccugcuau ugccuacaug     3180

aagcgggucu aucauuuuaa auucauguua auaucacagg aaguguacac acgcagagau     3240

gcaauguuca accagacuau cuauccagcc aaggaggcua auaggcggaa acucauugcg     3300

aucaagcacg guaaacccgu ugacguguac gggggcuaua gcggaucugu agaugccuac     3360

auggcaauug uccgaaucca cgacaagaag ggcgauaaau auaagguggu gggggucccg     3420

augagagcuc uggagauacu uaacgccacc caugacaaaa aggauuacga cgccaaacug     3480

aagcagaucc uaguuccuaa guuuacaaaa gugaagaaau cccgcaagac uggagaaauu     3540

accaaaaagg uagaggauuu cgaaaucgug cucgguaaag ucauguacag gcaacuggug     3600

auugacggcg auaagaaguu uaugguuggg aguagcacgu auuuguacaa ugcucgucag     3660

cugguguuau cugacuccuc acucgacaca cuggcaagua aaggaacccu ugauagcgac     3720

gccgauucga agcggcuggu cgcgguguau gacgagaucg uugaaaaagu ggauaaguac     3780

uucccauugu augacaaaaa cgcuuuuaga uccggccucc acaaggguag ggauaaguac     3840

cugcuacugc ccaaucgcuc uacuuucaac ggggccaaaa agguccauag cggcaaacga     3900

gagauacuua acgagauucu caauggacug cacgcaaacg ccacauuggg cgggcugaag     3960

gacuuaaaaa ucucaacccc uuuuggaaag augcagggcc agagugguau cccccucucc     4020

ucuaauacua uuauaugcua ucaaagccca accgggcugu ucgaacauag aguaucccuu     4080

aaggaucugu ga                                                         4092


<210>  3077
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3077
auggagaaca auagauacaa cgugggccug gacaucggga ccagcuccau uggauucgcc       60

gucacagaug aacaggacga gcucguucaa gugaagggua aaaauuauau aggcguaagg      120

cuuuuuaagg aagggcagac ugcuucugag cggcgcucau uccgaacgac ccguagaagg      180

cugaaacggc gcaaguggag auugggauua cuggaagaga ucuuugcacc cuaccuagcc      240

cagggcgauc cuuauuuccu cgcgaggaug aaagagagua acauuagccc acgggacgaa      300

cgcaagaagu acuaugguuc ccugcuuuuc ucugaugaca caguguucua cgauaaauau      360

ccgacugucu accaucugcg acacgcuuug augaccgagg acagaaaguu ugauaucagg      420

gaaauuuacc ucgccgugca ccauaucaua aaauaucggg ggaauuuccu gaucaacaca      480

cugauuaaug aguuuucguc aaaggcaguu gacuucaaaa aggaugccga cauccucaac      540

gauuuauuug agcaguucua cauuagcgac aucaaugaca guuuucaacu gaacaccgcu      600

gauaugaaug aaauauccga gcuuuugcug gacaacucua agacucgucu agaucgccag      660

agagaaagcc ucaaaaagcu ggcaaagaag guggacgaua uuuccgucgc caaaaagcac      720

cagaaaaucg cuacagaguu cucaaaggcc auucuuggaa auaaggcgaa auucgacgug      780

auccugaacg uaaccacgga uaguagcaau gacuggucuu uuaaguugga agaugaggac      840

gcagaugaga aaaucguugu gcuccuggaa gaguuaaaca cuccccagca agaagcccug      900

aacauuauac aucaguggua uucccagauc ucacucaaug gcauuguccc ugacggcaag      960

agcaucagug aggcuaugau aucuuccuac gaacuucaca aagagcaccu ggccuugcua     1020

aagcagcaaa uuaacaauac caaggacaaa aagaaagcac augggcugca cgaggcuuau     1080

aacgaauacg ugcauggaaa uaacaauaag aaacucaacc aggccgauuu cuauagcaag     1140

gugaagcaga aucuggaguc gucucuuauc acagacgaaa uccuggaguu gauuaacgcg     1200

gaaaauuuua ugccaaaaca aaggacuucc gccaacggug ucauccccca ccagcuccac     1260

cagcaggagc uggaucggau uauacaaaau caggcaaagu acuacccuuu cuuagcugag     1320

accaacccaa augaaaaacg aucaaagguu gccaaauaua agcuggacga gcuaguggcu     1380

uuucgcguac ccuacuaugu gggcccucuc aucacagcca ccgaucagaa gaaaacgagu     1440

gggaaggaau uugcauggau ggucagaaaa cagccaggaa ggauuacucc guggaacuuu     1500

gacgagaagg ugaacaaaau ggagagcgcc aauacauuca uccggcgcau gaccacuaag     1560

gauuccuacc ugaucgcuga agacguucuu cccgaugagu cuuugcugua ucaaaaauuu     1620

aaggugcuca acgaacugaa uaaccuuaga gcggacggca ggaagcugaa agucagcgug     1680

aagcagaaag uauucaagga guuguuucag cagcagaaaa caauuaccau aguuaagcuc     1740

caaaauuacc ugaaggcaga augcucauuc uuaacgaccc cugagaucag uggucugucc     1800

aaaccagagc auuuugugag cucucuagcc acauauauug aucucaagaa aaucuucggg     1860

gaagacauug auaaccccgc ucgucagaau gacuucgagc ggaugaucga augggccacu     1920

auauuugagg acaagaacau cuacucagaa aaacugaaug agauuaagug gcuuaacgau     1980

cgacagaaga aagaguugaa gaauaaaaac uaccacggau ggggcaagcu gagugcaaaa     2040

cuccugaagg gcuuaagaga cagcgaugaa uguuccauca uugagcauau guggaauacc     2100

caacagaacu ucaugcaggu ccaagccgaa gaggacuuug cucagaggau ccaggaagag     2160

aauaaccagc accugaaggg gaacacacuu gaggaagugu uggccgaugc auauacuucu     2220

ccucaaaaua aaaaggcgau acgccagguc gugaaaguua uugaggacau ccagaaggcu     2280

augggaggua uugccccaca gaaaaucucg auagaguuca cccgggcacc cgaagagaag     2340

ccgagaagga gcguggaacg ccaacagcag aucgagaaaa uuuacaaguc cguagccaac     2400

gaagucgaga augcuggcgu gaaggaugaa cuccagcgau uuucagaccg gaaacaaaca     2460

cugaccgaua aguauuuccu auacuuuacu cagcugggga aagacaugua uacgggaaag     2520

aguaucaaca uugaucagau cggcaauuac caaauagacc auauucuccc ucaggccuuc     2580

aucaaagaug acucucugga caaccguguu cuugugagcg cagcugucaa uaacgguaag     2640

uccgauaaug ugcccacaaa gaacgacaga uauaaaguaa gggagcacgg gcagcgcuug     2700

uacgaucagg gccugaucuc aaagcggaaa uuuaauaacu uaauuaccga cccagauagc     2760

aucaauaagu ucacugccag aggauuugug aaaaggcaac ucguugagac cucucagguc     2820

auucgacugg uggcggaguu ccuuuacaac aaguaucagu ccaagaguga aauaaucgaa     2880

gugcgcgcca aaaugaauag ccagcugcgg aaggacuuug gcuugauuaa aaacagaaac     2940

gucaaugauu accaccaugc ucucgacgca uaucugacaa ccuucaucgg gaacuaccua     3000

uauaggcguu acccuaagcu gcgcgccuau uucacuuacg gaugcuuuaa aaagguugau     3060

gacucucaac ucaaguacuu caauuuucug cacgauauug aggacgacaa aaagaaaauc     3120

auaaaggaua acgacacagg ugaaauccuu uggcaucggg augagggcau ugcuagauuc     3180

aaaaggaucu auaauuuuaa guacauguug gugacgcacg aggugcugac ccgacagggg     3240

gccauguuca agcagacugu auauuccgca gcggucgccg gaaguaaaaa guuaauuccc     3300

gugaaagaag accggccaau cgauauauac ggcgguuaua caaacaauaa cgacgcuuuu     3360

cucgccauug uuaaggugac caaaaagaaa caguacaugg ucguggggau cccccaacgc     3420

gcaguuguga agcugaauga ugcugccaag cagggcucaa aagcauauca gaagguacuu     3480

uacaacguca ucaaaccuca guucauuaag aaaaagaagg acagaaaaac aggaaagaua     3540

acucaaaaac uggagaauuu ugaaaucgug gugagcaaga cccuauacag gcagcucauu     3600

aucgauggcg accagaaaua uaugcugggg ucguccgccu acaaguauaa cgcuaagcaa     3660

uugguccugu cugaguacgc guuagaaguu cucgaucgga gcauuuucga gaaaaaggag     3720

uauacgaccg uggaccugga uaauguauac gacgaaaucc uugcccaggu ggacaaauau     3780

uuuucacugu acgauaagaa caaauuccga cagaaguugc acgagaguag aaagauauuc     3840

acagaacucc caaucaauaa ccuguuuucc ggaacuaaac uacugaaguc ugguaaacgc     3900

gagacccuua augagauucu cgaaggccug caugcaaacg cuacaaagaa agacuugaaa     3960

agccugaaau ucuccacucc guuagggaug auccaguacc ccucaggaau uauccucagc     4020

gagaacgcca uacuguguua ucaaaguccu accggcauuu uugaaaggcg ugucacgcuu     4080

aaggaucugu ga                                                         4092


<210>  3078
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3078
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uugguagcaa cuccauaggc       60

uucgccgcug ucgaugaaaa uucucaccuc aucagacuua aggggaaaac aguuauugga      120

gcaaggcugu uugaagaggg caaggccgcg gcugaccggc gcgccucacg aacuacgcgu      180

agaagguuga gucggaaccg cuggagacug agcuucuuaa gggacuuuuu cgaaucccau      240

aucaccccua cagauccaaa cuuuuucaug cggcagaaau auucugagau uucgccgaag      300

gauaagucac gcuacaaaua ugaaaagcga cuauuuaaug acagaacuga ugcagaguuc      360

uacaaauaug agaauugccc caccauguac caccucagga accggcugau gacagacccu      420

agcaaggccg augugcguga aaucuauuuu gcuauacauc acauccuuaa aagucgcggu      480

cauuuccuga ccccagggga cgcaaaggau uuuaauacua acaagguagc cgugaauaaa      540

auuuuccccg cuuugcaaga cgccuacgcg caggucuacc cugaucucga caucacauuu      600

gacgagaaca aggugaauga guucaaaacc guucugcuga acgagaaggc aacgccaucc      660

gauacucaga gagcccucgu gaauuuacug cuugcuaaag agggagacag ggauauuuug      720

aagcagcaaa aacaggcccu gaccgaguuc gcaaaggcug ucgugggccu aaagacaaaa      780

cucaacguag cccugggcac ugaaguugac ucuagcgaag cgaccgccug gaauuuuucc      840

cuugggcagc uggaugacaa guggacagga aucgagucag caaugaccga ugaagguacg      900

gagauauugg accagauucg ggaucucuau cgagcucgcc uguuaaacgg caucgugccc      960

gccgggaaaa cucugaguca agcuaagguc gacgauuacg cccagcacca cgaggaccuc     1020

gaacuuuuca aagacuaucu gaagcaguug gaggaugacg gaacagcaaa ggccauuaga     1080

cagcuauacg auagguauau cgacggcgau gacgcuaaac cuuuugugcg ggaagauuuc     1140

gugaaggcgc ugaccaaaga ggucacugca cauccaaacg ccaagagccc ggaacuccug     1200

gagcgccuug cucaacccga cuuuaugcug aaacagagaa auaaggccaa cggugcaauc     1260

ccuguucaga ugcaacagag ggaguuggau cagauuauaa agaaucaguc uguguacuau     1320

gacuggcucg ccgcuccaaa ccccguagaa aaacaccgua aguccaugcc uuaccaacug     1380

gaugaguuac ugaauuuccg gaucccauac uauguggggc cccuagucac agccaaagaa     1440

cagaaggcag cgcagggagg cguguuugcu uggaugguuc gaaaagaccc ggauggcaac     1500

auuaccccuu acaauuucga cgagaaggug gacagagaag ccucagcaaa cacguuuauc     1560

cagaggauga agaccacaga uacuuaucuc auaggggagg acguccugcc caaacaaagc     1620

cuuuuguacc agcgcuauga ggugcugaau gaacucaaca auguacgggu uaacaaugag     1680

aagcugagua uugaacagaa acagcaagug aucagagagc uuuucgagag gcauaacucu     1740

gucaccauca agcaguuugc cgaaaaucug cgcgcucacg gagauuacgc ccacaugcca     1800

gagauucgag guuuggcaga cgaaaaacgg uuccucucca gccugucgac auaucgucag     1860

uuaaagucuc ugcuaccuga ggcuaucgau gaccccgcca aacaggcgga uauugaaaac     1920

auaaucgccu gguccacugu guuugaggac gcugcaauuu ucaagaccaa gcucaaagag     1980

aucaacuggc uggauucaca aacaaucacc aaacuuagua auauuagaua caggggcugg     2040

gggcaguuca gccauaaauu uuugaacgga cugacucucg gcaaugguca cacgauaauc     2100

caggaacugu ucuuauccaa caauaaccug augcaaauuc uuacagacga gaccuugcag     2160

aagaaaauga cugaacucaa ugccgauaag cugaaggcug ccaacaucaa ugacgcaauu     2220

gaugacgucu acaccucucc aagcaacaaa aaggcgcuac gccaggugcu gcggguuauc     2280

gaggacauaa aaagagccgc ugaugggcag gaccccucau ggcucuaugu ggaaacagcc     2340

gauggcggag gcaccccugg gaagaggacu cgagcacgcc aacaucagcu gcaggagauc     2400

uacgcuaaug ccgcacacga gcuuauugac agugccguac ggggagaauu ggaggauaaa     2460

aucgcugaca aggcggauuu uaacgacaga cuggucuuau auuucaugca ggguggcagg     2520

gauauuuaca cagggacgcc acucaauauc gaccaacugu ccagcuauga uauagaccau     2580

auucuuccgc agucucugau caaggacaac ucauuggaua aucgugugcu cguucacgcc     2640

auuaucaacc gcgaaaagaa cgcaaccuuu gcuaguacua uauacgccca gaagaugaau     2700

gccacauggc ggcaguggca cgaggcugga cugaucagcg gcagaaaacu aaggaaccug     2760

caaaugcgac ccgaccagau ugauaaguau gcauccgguu ucguggcccg gcagcucguc     2820

gaaacccgcc aaaucauuaa acugacagag cagaucgugg cggcucagua cccugagacu     2880

aagauaauug ccguaaaggc agggcuuucu caucaguuga gagaggaacu ggaauuuccc     2940

aaaaauaggg acgugaacca cuaccaucac gccuucgaug cauuuuuagc ugcccggauc     3000

ggcaccuauc uccugaagcg auucccaaau cuucaagcgu uuuucacgua cggaaaauuu     3060

aagaaagcug acguuaagaa acugagaggc uucaacuuca uccgcgauau uacccacgcc     3120

gaggacaaga ucgucgugaa ggauacaggg gaggugauuu gggacaaaaa gagggauguc     3180

gacgaacuag aucguauaua uaauuuuaaa cggaugcuca ucacucauga gguucacuuc     3240

gaaaaggcag accuguuuaa acagaccgug uacggagcca aggacucgaa ggaggcuggu     3300

ggcaaaaagc aguugauuag caaaaagaaa ggguauccug uggauaucua cggaggcuau     3360

acacaggaaa cugguuccua ccugucagua gucagauuaa ccaagaaggc cauguacgca     3420

gugguuaaag ugaguacgag ggacgcugcc aagcucgcgg ucgccaaauc uauuagcgag     3480

caaaaggaga acgaaacacu gaaugauauc auagacgaga aacuuuccaa gaucucaaag     3540

aacgggaaaa uuacccauga acuguucgag gugguuuugc cacgcguggg ccagaagacu     3600

cucuuuaaaa auagcaagua uaaccuguuc cuaguaaauu cugauacaua caugcacaac     3660

uaucaggaac uguggaugcc ccgagaguac cagaaaaugu ggaaggacau cucccuuagu     3720

aaguauggag augcacaaac cgaggaccag cucgaucaga uuuuuggcuu caucgucagc     3780

caggucaauu cuuacuuuaa ccuguaugac auaaaccaau uccggaaaaa guugaaugau     3840

gcugccgaca aauuugcuac ucugccgaug agagauaccg acgacaugca ggggaagauu     3900

gcaacaaucg gacaguuacu caucggccug caagccaacg ccgcuagguc cgaucuucgc     3960

aaucuggaca uuaaaacgcc uuugggucuc cugcagauac caaacgggau cucacuagau     4020

agugacacca gcguggugua ccagucgccc acuggacugc uugaacguga gguucaucuc     4080

uccgaucugu ga                                                         4092


<210>  3079
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3079
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccauaggu       60

uucgccgugg ucgaugcuaa cucucaccuu cugagauuga aaggcgagac agcaaucggg      120

gccagguuau uucgggaagg acaaucagcg gcugaccgcc gagagagucg uacuacgaga      180

aggcggcuga gccgcaccag auggaggcua uccuuccucc gggauuucuu cgccccucau      240

auuacaaaga ucgacccaga cuuuuuccug cgccagaaau auucugaaau uucgccgaag      300

gauaaagauc gauuuaagua cgagaagaga cuuuucaaug acaggacuga ugcagaauuu      360

uaugaggccu accccucaau guaucaucug cgguugcguc ucaugaccga cgcuagccag      420

gcagauccuc gcgagaucuu ccuggccaua caccacaucc ugaaaaguag aggccauuuu      480

cucacaccag gugcugccaa ggacuucaac accgaugaag uugacuuaaa acacauuuuu      540

cccgcgcugg cagaggccua cgcucaggug uauccugacc uugaauugaa uuucgauucc      600

gcccuggcag acgauuucaa ggcuaaacua cucgacgagg augccaagcc aucugacauc      660

caaaaggcgc ugguaaaccu ucuguugagc uccgaugggg agaaagaaau ugugaagaaa      720

aggaagcagg uccucacuga guuugccaaa gcaaucacag gacugaagac caaauucaau      780

uuagcucugg gcacggaagu ggacgaggcc gaugcuucaa acuggcaguu uaguaugggc      840

cagcucgacg auaaguggag caauauagaa acuucuauga ccgaccaagg gacagagauu      900

uucgagcaga uccaggaacu uuaccgggcc cgacuguuga acggaauugu ucccgcaggu      960

augucccuau cacaggccaa gguggcugac uacggccaac acaaagagga ucuggaacuc     1020

uuuaagacuu auaucaaaaa gcugaaugac caugagcuug cgaaaaccau ccgcgggcug     1080

uacgauagau auauuaacgg agacgaugca aagccuuucu ugagggaaga cuuugucaag     1140

gcccucacaa aagaggugac cgcucaccca aaugagguaa gcgaacagcu guuaaaccgg     1200

augggccagg ccaauuucau gcugaagcaa cgcacgaaag caaacggugc cauaccgguu     1260

cagcuacagc agagagagcu cgaucaaauc auugcuaacc agaguaagua cuaugacugg     1320

cuggccaaac ccaauccugu ggaaaagcau agggcaucua agccauacca gcuugaugag     1380

uugcugacuu uucguauccc cuauuacguc gggccucuca uaacaccaaa acagcaagcg     1440

gaauccggag agaacguguu cgcuuggaug gugcggaaga aucccagcgg caacauuacc     1500

ccguacaauu uugacgagaa agucgaucga gaagccucgg caaacacuuu cauccagaga     1560

augaagacaa ccgacacgua ucugaucgau gaggacguuc uuccuaaaca gucucuguug     1620

uaccagaagu augaagugcu caaugagcug aacaauguaa ggauuaacaa ugaggccuua     1680

uccaccgacc aaaagcagcg ccugauccgg gaaguguuug agagacacuc aagugucaca     1740

auuaaacagg uggcugauaa ccuaguugcc cacggcgacu ucgcacagag gcccgaaaua     1800

cgcgggcucg cugaugagaa gcgauuccug agcucccuuu cuacuuacca ucaauugaaa     1860

gaaauccugc acggagccau ugacgaucca accaagcucc augacaucga gaauaucauu     1920

acauggagca cuguguuuga ggaugcggcc auauucaaaa ccaagcuggc ugaaaucgac     1980

ugguuagauc cugcaacaau uaaaaagcug ucaaacaucc gguaucgugg uuggggccag     2040

uuuaguagaa aacuuuugga cgggcucaaa cugggaaaug gccacaccgu cauucaggag     2100

cuaaugcugu ccaaccauaa ccucaugcaa auccuggccg augaaacucu uaaggagacg     2160

augacagaau ugaaucagga caaacugaag accgaugaca uagaggacgu gaucaacgau     2220

gcuuacacua gccccucuaa uaaaaaggcc uuaaggcagg uacuccgcgu uguggaggac     2280

auuaagcacg cagcgaacgg ucaggaucca ucauggcugu auaucgaaac cgccgacggg     2340

cccggcacag cuggaaaacg gacccaaagu agacagaagc agauucagac ugucuacgcc     2400

aaugcagcuc aagagcuuau cgauagcgcc gugaggggcg aacuggagga caaaauagca     2460

gauaaggccu ccuucacaga ccgauugguc cucuacuuua ugcaaggggg acgcgauauu     2520

uauacgggug cuccucugaa caucgaccag cuaucucacu acgauauuga ccauauccug     2580

ccacagucgc ucauaaaaga cgauagccug gacaaucggg ugcuuguuaa cgcgaccauc     2640

aauagagaaa agaacaaugu guucgccucc acuuuguuug caggcaagau gaaagcuaca     2700

uggaggaagu ggcacgaggc cgggcugauu ucaggacgua aauuacgcaa ccucaugcug     2760

cggccggaug agaucgacaa guucgccaaa ggcuuuguag cuagacagcu ugucgaaacc     2820

aggcagauua ucaagcugac agagcagaua gcagccgcgc aauaucccaa uacuaagauu     2880

aucgcuguga aagccggucu aagucaucag cuccgagaag aacuggauuu cccuaagaac     2940

cgggacguua aucacuacca ccaugcauuu gaugccuucu uggcugcacg caucgggacc     3000

uaucuguuaa aaagauaccc aaagcucacg cccuuuuuca ccuauggcga guucgccaaa     3060

guggacguca agaaauuuag ggaauuuaac uuuauuggag cgcugacaca cgcuaagaag     3120

aacaucauug ccaaagauac uggcgagaua gugugggaca aggaacggga uauccaggag     3180

cuugaccgaa uuuacaauuu caaaagaaug cugaucaccc augaaguaca cuuugauaug     3240

gcagacuugu ucgaccagac aauuuacgcc gcuaaggauu cuaaagagcg cgggggaagc     3300

aagaaccuca uccaaaagaa acaggguuau cugacuggca uauacggggg auauuccacc     3360

gagaggggcu cauacgugag caucauucgu acguuuaaga aaggugcccc uaucuauaau     3420

auuguuaaca ucucuacagc agacgucucc cuacugagug auaagcagcg ggugagcgag     3480

gcuucugccg acgugcuucu caaagagcug auaaagaaga aauuggcgaa gagaaaaucc     3540

ggggaaaaua ccaagucagg cgccuucgag auuguccugc caaggguugg aaagaacacu     3600

uuauuuaaga auaagaaaua cggccucuuc cugguggaaa guacaaccga guuucauaac     3660

uaucaggagc uuuggcugcc ccgcaaugau caaauguuga ucaagagcau ccagucgcac     3720

aaaauugaag aguccaaggc agacuacgaa cucacucgaa uguacagaaa cauagcuucu     3780

cagguggauc acuauuucgg gcuguacacc auccggaaau ucagcgagaa gcuaacagcc     3840

ucaauuaaug acuauggagc ucugccggua uacaagagug aaacgaccga uggcaaauau     3900

gagguccuuu ccaaccucuu ugagggucug gggucuggaa cuagcacagg caauuugacc     3960

gcagugaagu uacagccuaa cuucgguagg guuuaccuga cauccggcau cgugcucgaa     4020

gacgauacuu caauuaucua ucaaagccca accgggcugu uugagcgcag ugucaaacuu     4080

gccgaccugu ga                                                         4092


<210>  3080
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3080
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccauaggu       60

uucgccgugg ucgaugcuaa cucucaccuu cugagauuga aaggcgagac agcaaucggg      120

gccagguuau uucgggaagg acaaucagcg gcugaccgcc gaggcagucg uacuacgaga      180

aggcggcuga gccgcaccag auggaggcua uccuuccucc gggacuuuuu cgccccucau      240

auuacaaaga ucgauccaga cuuuuuccug cgccagaaau auucugagau uucgccgaag      300

gauaaagauc gauuuaagua cgaaaagaga cuuuucaaug acaggacuga ugcagaguuu      360

uaugaagacu accccucaau guaucaucug cgguugcacc ucaugaccca cacacauaaa      420

gccgauccuc gugagaucuu ccuggcuaua caccacaucc ugaaaagccg cggucauuuu      480

cucaccccag gggcagccaa agacuucaac acugauaagg uugacuuaga ggacauuuuu      540

cccgcucuga cagaagccua cgcccaggug uauccugauc uugaguugac cuucgaccug      600

gcaaaagccg augacuucaa ggcuaagcua cucgaugaac aggccacgcc aagugacacu      660

caaaaagcac ugguaaaucu ucuguugucc ucugauggag agaaggagau cgugaaaaag      720

agaaaacagg uccucaccga auuugcuaag gccauuacag gccugaaaac uaaguucaac      780

uuagcgcugg gcaccgaggu ggacgaagcc gaugcaagca auuggcaguu uuccaugggg      840

cagcucgacg auaagugguc aaacaucgag acaaguauga ccgaccaagg aacggaaaua      900

uucgagcaga uucaggagcu uuacagggcu cggcuguuga augguaucgu ucccgccggc      960

augagccuau cucaggcuaa aguggccgac uacgggcaac acaaggaaga ucuggagcuc     1020

uuuaaaacuu aucugaagaa acuuaacgac caugaacugg caaagacaau ucgaggauug     1080

uacgaucgcu auaucaaugg cgacgaugcc aagccuuucc ucagagagga cuuugucaaa     1140

gcucugacca aggaagugac ugcgcaccca aacgagguau ccgagcaguu acugaauagg     1200

augggucagg caaacuucau gcuaaaacaa cggacaaagg ccaauggggc uaucccgauu     1260

cagcuccagc agcgcgaacu ggaucaaaua aucgccaacc agucaaaaua cuaugacugg     1320

cuugcagccc ccaacccugu ugaggcucac agauggaaga ugccauacca guuggaugaa     1380

cugcucaauu uucauauucc cuauuacgug ggaccucuga ucaccccaaa gcagcaagcc     1440

gagagcggcg aaaacgucuu cgcauggaug gugaggaaag accccagugg caauauaacg     1500

ccguacaacu uugaugagaa gguggaccgu gaggcgucug cuaauaccuu cauucagcgg     1560

augaaaacaa cugauaccua ucuuaucggg gaagacgucc ugccuaagca guccuugcuc     1620

uaccagaaau augagguucu gaacgaauua aauaacgugc gaaucaauaa cgagugccug     1680

ggaacagacc aaaagcagag acuaauuagg gagguauuug aacgccacag cucggugacu     1740

aucaagcagg ucgccgauaa ucucguggca cauggugacu ucgcccggag acccgagauu     1800

aggggccugg cugaugaaaa acgcuuccuu ucuuccuugu caaccuacca ccagcugaag     1860

gagauacucc augaagccau cgacgaucca acaaaacugu uagacauuga gaacaucauc     1920

accuggagua cuguuuuuga ggaucacacg auuuucgaaa caaagcuggc agagauagaa     1980

uggcuugacc cuaaaaagau caaugaguug agcgggauuc gauaucgggg auggggccaa     2040

uuuucccgua aacuccugga uggucuaaag cuggggaacg gccacaccgu gauccaggaa     2100

cucaugcugu cuaaucauaa ccuuaugcag auuuuggcug acgagacucu gaaggagacc     2160

augacagaau uaaaccaaga uaaacucaag accgacgaua ucgaggacgu cauaaaugac     2220

gccuacacua gccccucaaa caaaaaggcg cugagacagg ugcuuagggu aguugaagau     2280

aucaaacacg ccgcuaaugg acaggaccca aguuggcugu ucauugagac agcagauggc     2340

acggggaccg ccggaaagcg cacucagucc cggcaaaagc agauccagac aguguaugcu     2400

aacgccgcac aggaauugau ugacagcgcg gucagaggug agcucgagga uaaaaucgcc     2460

gacaaggcuu cuuuuaccga uaggcuggug cuauacuuca ugcaaggcgg gcgagacaua     2520

uacacaggag cgccccugaa uauugaucag cucucacauu augacaucga ucacauucug     2580

ccucagaguc uuaucaaaga cgacagcuug gauaaccgcg uccuggugaa ugcaacuaua     2640

aaccgggaaa agaauaacgu uuuugcuucc accuuauucg ccggcaaaau gaaggcaacg     2700

uggagaaagu ggcacgaggc cggucucauc ucugggagga aacugcguaa ucuuaugcug     2760

cgcccagacg aaauugauaa guuugcuaaa ggcuucgugg cgcggcagcu aguagagacc     2820

agacaaauca uuaagcucac agagcagauc gccgcagcuc aguacccgaa cacuaaaaua     2880

auugccguca aggccggacu gucgcaucaa uugagggagg aacuggacuu ucccaagaau     2940

cgagauguga accacuauca ucacgcuuuc gacgcauuuu uagccgcgcg gaucggcacc     3000

uaccuccuga aacgcuaucc uaaacuugcu ccauucuuca cauacgggga auuugccaaa     3060

guugauguga agaaauucag agaguuuaau uucaucggag cacugacuca ugccaagaaa     3120

aacauuaucg cuaaggacac cggugaaauu gucugggaua aggagaggga cauacgggag     3180

uuggaucgaa ucuauaacuu uaaaagaaug cucauuacgc acgaagugua cuucgagaca     3240

gcagaccugu uuaagcagac caucuacgcc gcgaaagaua gcaaggaacg cggcgggucc     3300

aaacagcuaa uucccaagaa gcagggauau ccuacucaag uauacggcgg uuauacacag     3360

gagucaggga guuacaaugc ucuggugagg guugccgaag cagacaccac ugccuaucag     3420

gucaucaaaa uaucugcuca gaacgccagc aagaucgcau ccgcuaaucu uaaaucacgu     3480

gagaagggca aacaacuccu gaacgagauu guggugaagc aguuggccaa gcggagaaag     3540

aauuggaagc caagcgcgaa cucuuucaaa aucgucauuc ccagguuugg aaugggcacc     3600

cuguuccaga augccaagua cggguuauuu augguuaacu ccgacacaua uuaccgcaau     3660

uaccaggaac ucuggcugag ucgagagaac caaaaacuuc ugaagaaguu guucagcauc     3720

aaauaugaaa agacgcagau gaaucacgau gcacuccagg uguacaaagc uauaauugac     3780

cagguggaga aguucuuuaa acuguaugau aucaaccaau ucagagccaa gcuaucugac     3840

gcuaucgaac gguuugagaa gcugccgauu aauaccgaug gaaacaaaau aggcaagacu     3900

gagacacuua ggcagauccu cauuggucug caggcaaaug ggacccgcuc caacguaaag     3960

aacuugggaa ucaagacaga cuuaggccug cuccaagucg guucaggcau uaaacuggau     4020

aaggacacuc agaucgugua ccagaguccu agcgggcuuu ucaaacguag aauaccacug     4080

gccgauuugu ga                                                         4092


<210>  3081
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3081
augggcgaca gaaaguacaa ccuggggcuc gauaucggaa ccagcuccau ugguuucgcc       60

gcuguggacg agaauaacca gcccauaagg gucaaaggca agacagcaau cgggguucgg      120

cuuuuugaag agggaaaaac ugccgcggau cgccgaggcu uccguacgac cagaaggcgg      180

cugucucgca gaagguggcg gauuaauuug uuaaacgaaa ucuuugacgc ucaccuggcc      240

gagguggauc cuacauuccu agcacgccuc aaggaaucaa aucgaaguaa ccuggacccc      300

aaaaagagcu uucaagguuc ccuucuguuc ccggagagaa aggauuauca guuuuacgag      360

gaauauccca cuauuuacca uuugaggaaa gcccucaugg agaaggaccg gaaauucgau      420

uuucgugaaa ucuaucuggc uguacaccau auaaucaagu accgcgggaa uuuccugaac      480

ggaaccccua ugagaucuuu uaaaguggag aauauugagc ucgacacauu auucgaucag      540

cugaaccagc uuuaugcaga aaucguccca gacaaugagu uggccuuuga ucuggcucaa      600

guggccgacg uuaaggacgu gcuaucguca accacuauuu acaagaugga uaaaaagaaa      660

cagcucguca agaugaugcu gcuucccgcg agcaacaaag cacugcagag ugaaaauaag      720

aaaaucguga cacaguucgu aaacgccaua uugaauuaca aguucaagcu cgacguucug      780

uuacaagugg agaccgaugc ugacuggucc cugaaacuca acgaugaagg cgccgacgau      840

aaacuugagg aguuuacggg cgaccuggau gaaaacaggu uggagauuau cgaccuacug      900

cagcggcucc acaauugguu cucucugaac gaaauuacua aagaugggaa uagccuuucc      960

gcagcuaugg ucgagaagua ugaaaaccau caccaccauc ugggauugcu caaaaaggug     1020

aucgagaauc acccugacgc caagaaagcg aaggcccuga aagagaccua cacagcauau     1080

guggguaaga cugacgauaa aacccagaac caggacgauu uuuacaaggc ugucgaaaag     1140

aauuuagacg auucaccaga cgccaaagag aucaagcgac ugauucaacu agaucaguuc     1200

augcccaaac agcgcacagg ccaaaacggg gcuauaccuc aucagcucca ccagcaggaa     1260

cuggaccaaa ucauugagaa gcagaguaaa uauuacccau uucuugccga accgaauccc     1320

aacguuaaga gaaggaagga ugcaccuuau aaauuggacg agcugaucgc cuucaagaua     1380

ccauacuacg ugggaccccu cguaaccccu gaggaacagg cucagaaugg cgagaacgug     1440

uuugcgugga ugaaacggaa ggcagccggu ccaauuacgc ccuggaauuu cgaugaaaaa     1500

gucgaccgca uggagagcgc uaacagauuu aucaggcgua ugacuacaaa ggauaccuau     1560

cuguucgggg aggacgugcu uccggccgaa ucuaugaucu accaaaaauu uguugugcug     1620

aaugaguuga acaaccucaa gauuaaugga cggcaccugu ccuuaaagga caaacaggau     1680

gucuauaacg accuguucaa gcagcagaaa acugugucaa ucaaggcacu acaaaauuac     1740

uauguaacaa agaagaaggc cgcuaccgcc ccuacgguug gcggccucgc agaucccaaa     1800

aaguuucuga gcagucuuuc uaccuacauu gacuucaaaa acauguucgg ggaacgagug     1860

aaugauccac aguuucagga ggacuuggaa cagauagucg agugguccac aaucuucgag     1920

gauagaggaa uuuuuaaggc gaaacugcaa gcucucgguu ggcugagcga aaagcagauc     1980

cagcaauuag uggccaagag guauaaaggc ugggggcgcc ugucgaagaa acuuuugacu     2040

ggacucaaga acgcagaggg cuacucuauc cuggacgaaa uguggcgguc caccgguaau     2100

uucaugcaga uucagucaag accugaguuu gccgcucuaa uacagcaagc caacgaaaaa     2160

caguucgagg ggaaugaucc cgacaacguc ugggagaaua ucgaaaacau ucugggcgau     2220

gcauacacaa guccacagaa uaagaaggcu auccgccagg ugguuaaagu gguacaagac     2280

auugagaagg ccgucggaaa cccaccugaa aagaucgcga uagaguuuac uagggaagcc     2340

gcugcaaauc cacagcgaac ccagagccgg cuccguacac uggagaaacu uuaugagucc     2400

gccgaagagg ugguugaugc uggcuugacc gccgaacugg cagaguucaa agagaacaag     2460

cauguguuau cugacaaaua cuaucucuac uuuacucagc uggggagaga cgucuauacg     2520

ggagauacaa ucagccuuga caagcugaac gauuacgacg uggaucacau uuugccgcaa     2580

ucauucauca aggacgauag ucucgacaau aggguacuga ccauucgcgc ggugaacaau     2640

gguaaauccg auaacguucc cgccaagaug uuuggcaaaa agauggggag cuucuggcgg     2700

uaucuacugg acaauggaau gaucucuaaa agaaaguaca acaaucucau aacugauccu     2760

gacaacauuu caaaguacgc ucagaaaggc uuuaucaaua ggcagcuggu cgaaaccagu     2820

caagugauua aacuuacagc caacaucuug aaugguauau augacaagga uaccgagauc     2880

auugaagugc cagcaaagau gaacagccag augcgaaaaa uguucgaccu ggucaagguu     2940

cgcgagguga augauuacca ucacgcuuuc gacgccuauu uaacuaucuu uauugggaac     3000

uaccucuaua agugcuaccc caaacugcag ccuuauuucg uguacgauaa uuuuaagaaa     3060

uucggcaaca aggaagacau cggacauaaa cgguuuaacu uccuuggcaa gauagagaga     3120

gagaagaaag uagucgcacc agaaacaggg gagauucugu gguccaaugu ggcccccaac     3180

gaaacgauca agcagaucaa aaagguuuac gauuauaaau uuaugauugu gucuagggag     3240

aucaccacuc gucgcgcuga acuauucaau caaacagucu acccgaagaa cuaucacgga     3300

aagcucauuc cuauaaaaga ggaccggcca accgaucugu acgguggcua uucggggaau     3360

acagacgcgu acuuggccau cguggcacug gaggauaaga aaaagggaaa auauuuuaag     3420

guugugggca uucccacuag aguagcugcc aaauuagaaa agcucaagca gcaggacagc     3480

cagcaauacc ugcaggcccu ucacaaaguc aucgcuccuc aguucaccaa guccacgaaa     3540

aaggguauua aaaagaccga guuugaaauc gugcuggaua aggugcauua caggcaguug     3600

guccaagacg ggcccguuaa aaugaugcuc ggcucaagua cauauaagua caacgcaaaa     3660

cagcuggugc uaucugagaa ggcccugcag guaauagcgg acgaucgaaa auucgacgaa     3720

acucagaagg augacaaucu uaucgcugug uaugaugaga uucucagcau cgucaaccaa     3780

uccuucgacc uguacgauau uaauggauuu cggaagaaau ugaacgacaa ucgcgaucag     3840

uucaucgacc ugccagccga gaccaaguau gaaggcagaa aagugguugc acacgggaag     3900

agggagauga uauuagaaau ucucaaagga cugcaugcca acgcugcauu uggcaaucuu     3960

aagccuaucg guuucucaac agccuuuggg cagcugcaag ugcccaacgg aaucauuuug     4020

agcaagaaug cgauacucau ccaccagucu ccauccggcc uguucgagcg gaaaauuaag     4080

cuaagugauc uguga                                                      4095


<210>  3082
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3082
auggagaagc aggaauacua ucugggccuc gacaugggga ucagcuccgu gggaugggcc       60

gucaccaacg auaaauacga gcuucugaga aagaaaggua aggacaugug gggcauuagg      120

gaguucgagg aagcugauuc uucagcagag cggcgcacac accgaguuaa ucguagaagg      180

cggcaacgcc agguggccag aaugggguug uuaaaagagu auuuuaguga cgcgauagaa      240

aagguagaua gcaacuucuu uaucaggauu gaaaauucca aguacuucuu ugaagacaaa      300

gauggacggc ugucuucgaa gaacggcauc uucgacgaug cugacuauaa agauguggac      360

uacuauacug aguaccccac gauuuuucau cuacgcaagg cccuccugga uaucgaggac      420

accgguaaag aauacgacgu ccgacuugug uaucuggcau ugguuaauau guucaagcac      480

agagggcauu uucuccugaa cacagaggga gaugcccugu caagcgaaca ggugaauacu      540

accuaccagg agcucuuaga aauacuggag aacaauuaug acaucagucu ugaguccagg      600

gauauuaagg acgucaucga uauuaucucu agcuccgaca acggccggaa aaagaaacac      660

gaacaaauau uggagcuguu cggcgugaag aaaucagaua agcgugcuaa ugaacuacuc      720

aaaugccugu gugggcuuag uguaaacgca aagcugauuu uugacgagaa uuacgaagag      780

aagguugauu ugugcuucca ugacuuuagc uauacagaua aaaucccuga ccucauggag      840

aagcugucug aagaggaaua cuaugugguc gagcaguuaa aauccauuua cgacuacuca      900

auccuggccg gaacccucaa ggguuaugau uaccuuagcu gugcucgcgu ggaacuguau      960

gagaaacaca gagaggacuu gaagcuacug aaggcccucu acaggcggua uaaaacuaac     1020

gaagaguaca augauauguu caagagugac aaagaaggca cauauucugc guacgugaac     1080

uccuucaaca gcucggauuc uuccgucaag aucaaaaagu accgauggaa uaugaagggg     1140

cgcaccauug acaaccugua uggaacgauc aaaaaggcau uuaaagauaa uacugacgau     1200

aaggacguuc agucaaugau ggagaguaua gauaaagagc aguuccuucc aaagcaacug     1260

accggcgcca acggugugau uccgaaucag guacacaaga aagaaaugga gagaaucuug     1320

aagaaagcuc agaacuaccu ccccuuucug aaggaaaaag acgagagcga auuaacagug     1380

uccgagagga uacugcggcu auucacuuuu cagauuccuu auuacgucgg gccacucgga     1440

gaaaauucua agaccggcug ggugguucgc agagaaggcg ggagggugcu gcccuggaac     1500

aucgaugaga aaaucgacau gagcgccaca ucagaagcau ucauuggaaa ucuuauccgu     1560

aagugcaccu auuugaguga cgagaagguc augccuaagg cuucccugag cuacgaacgg     1620

uauugugugc ucaacgagau uaauaacaua ugcaucgaug gugagcgacu ggacacggaa     1680

cuuaaacaga aaauuuacac ugaguguuuu augaagggca aaagaguaac aaggaagcaa     1740

aucugcaauu accugguugc ccaggggugu aucaagucug augaacaagu gaccggaauu     1800

gacauaaacg ucaauucaag uuugagcucc uauggcaaaa uguucgcgau cuuuggugag     1860

caucucaagg aagaugccau ucgcaaagug gcagaggaga ucauuuacug ggggacuauc     1920

uucucugacu cgaagaaaau guuucggaag caccugacaa aguaugucga acagggcaua     1980

uuaggagaga gccaggugaa aagaauccug ggcuacaagu ucaaagauug ggggagguuu     2040

uccaaggcuc uacucacccu gcagggaauu gacaaaucaa cgggugaacu uuugagucug     2100

gagaacgcca ugugggagua uucucucaac uucauggaac ugaucaauag cgaugacuuc     2160

accuuuaagg augaguuagc uaaccgccga acauccgcca uuaagucacu gagcgaguuc     2220

acuuacgagg accuugaaga uaccuauuuu ucuguuccag ugaaacggau gaucuggcaa     2280

acaauauugg uaguccguga gauugagcag aucaugggcu acgcacccaa gaaaguguuc     2340

guugaaaugg ccagauccga ggaagagaag ggggacaaag gaaggaagga cagucgcggc     2400

acucagcucc uggaacuaua caaaaauauu aagaacaccg agaaucauga uuggaagaaa     2460

gagaucagcg aagcuaacga gucugguaag cugcggucca aaaagcucua ucuguacuau     2520

acacagaugg ggagagacau guacaccggc gaagagauaa auaucgauau gcuuuaugac     2580

gauucaaugu acgacauuga ucacaucuau ccuaggcacu acgugaaaga cgauaguauu     2640

gucaacaauu uggugcuggu aaaccgacgc gcgaaucaag acaucaagaa ggauuuauac     2700

ccaauaccgg acaaaauuau cagcaacccc aaggugcagg aacucuggaa aaugcugcau     2760

aagcagaauc uuaucacuga cgagaaauau aagcggcuga ugucgagauc cccuuuuacg     2820

gagaagcagu ugggagauuu cauugcaagg caacucguug aaacacguca gggcaccaaa     2880

gggaucgccg agcugcuagg acagcuguuu aaggacacug aaauugucua cgcuaaagcc     2940

accaacgugu cugauuuccg ccgggacaau gguuuuguca agagcagacu cgugaacgau     3000

uuccaccaug cacaggacgc cuaucugaac auaguugugg gcaaugugua cuauacaaaa     3060

uuuaccaaga acccauggaa uuucaucaag aaagauuuug acaaggauag gaacaaaaau     3120

ggguacaacc uugcuaagau guucgaccga gauguagucc ggggagacga uacugccugg     3180

auugcaagua aaaaggacac acaaggcacg aucgagaccg ugcgcaagau gaugaggaaa     3240

aauacucccu ugaugacaag aaugucauuc gagcagcacg gugggauugc gaacgcuacc     3300

cuguauuccg ccaagaaagc aaagguugaa aauuacaucc cuuuaaaauc uagcgacaua     3360

cggcucuccg augugaagaa auacggcgga uuuacaucag ccacugucgc uuauuacuuc     3420

aucgugcauc acaaggaggg caagaaagaa aacaccacga uugaggcccu gccacuuuau     3480

ugcaagcgaa aaguugaaaa ugaggagaac gggcugcuaa aguacuguga agaggugcuc     3540

ggauauggcg acguaaaaau ccuguuuaau aaguugaaga uucagagccu guuagauauc     3600

aacgguuacc aagcacaccu cagugggaaa accggaaauc agcugacacu uagaaacgcu     3660

augaaccugu gcguccccga aaaguggacu gccuauauua aaaaguugga gaaacucgug     3720

gugggccaug ucaaggaaca ggagauaucu uccgaggcga auaucgaacu guacgaccua     3780

cugaccgaua agcaccuuaa cagcguuuac gccaaacgcc cgaaugcuua uggucagaag     3840

cucaucgaca acaggucgcg uuucgugaau cugaaaccug agucacaaug cgaagcaauu     3900

uugcagauac ugaaguuaac aaaaaucggc aacaaugaga cuaaccucaa gcugauuggg     3960

gaaucugagc auaguggaaa gaugcuuauc uccaaaaauc ugaaggauac cgacaaaccc     4020

gccauuaucu gucagagcgu aacgggcuug uacgagcgga agauauauau cuuugaugaa     4080

cagcucgaca aauga                                                      4095


<210>  3083
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3083
augaacaaga gauacaauau cggccuggac auugggacca acagcauagg augguccgug       60

gucaaugauc agucucaacu cguuaggauc aaagguaaac uuggcuaugg ggugcggcug      120

uacgacgagg gacagacagc cgaagaucgc cgauuguucc guacuacgag aaggcgguua      180

aaacgcagaa aguggaggcu gggccuacuc cgggagauuu uugaacccua uaucaccccu      240

caggacgcua cauucuuucu gcgccagaaa caaucaaacc uuaguccaaa ggaucugcag      300

aagcagcagc cgcaaauugg uuuguucaau gaccgacucg auagcgacuu ucacuaccag      360

uaucccacua ucuaccaucu gagauaugca cugaugaccg agaaaaggca guucgaucuc      420

cgggaaauau acuuagccau gcaccauauc guaaaguacc gugggcacuu ucugaacgcg      480

gcugccgcau ccucuuucaa aucgggagag auugaccuug ccaaggauuu ugcugcauug      540

aaucagaucu ucggcacaau cuuuucagag gugggcuuca gccugaguga auccaaccua      600

gacgagauua aaucuauacu ccuggauaau aaccuuagcg ccuccguccg ccaaagaaag      660

gcucugccuu ugauuuauca gaccgccgcg ucaaaggaca gugaaaaaca guacaaggca      720

aucgccacug agcuccugaa agcuauuuua gggcugaagg ccaaauuuaa ucucaucaca      780

ggaaucgagg uggaagacgu uaaggcaugg acccuuagcu ucaacgcuga ggauuucgac      840

gccaaacugu cugauuugaa uauugcggac aacgaugacg ccgugcaaau aaucgaaauu      900

cuaagggcac uguauuccgg uguccuccug acgggcaucg ugccagaggg gcagucaaua      960

agccaggcua ugauugauaa guacgaagcc cauaaggcuc accuuaucau gcugcggagu     1020

auccgagagc aguugucuga caaagagaau aaggcccucg aaaaagcaua ugaugacuac     1080

auugaucacc ccgaggacaa gcaacaugga ucccgcgaag acuuuuaugc cgcuaucacu     1140

aaaguacuga agaaggauag cgaggacacc aacauuauag aaaucuuaaa acugauugag     1200

cuagaucagu ucaugccuaa gcagagaaca aaagacaacg gcgcgauccc acaccagcuc     1260

caucaacagg agcuggauca gaucauugaa aaucagaagc aauacuaccc cuggcuugca     1320

gacauaaacc cuaaugagca gaggcagaaa auggccccau auaaguugga ugaacugguu     1380

acuuuucggg ugccguacua ugucgguccc augaucaccg cugaggaaca guuccaaaca     1440

ucgaacaaga aauuugccug gaugauucgc aaggagccug gggcaaucac cccauggaau     1500

uucgaccaga aaguggauag acaggccucu gcuaacgagu uuauuaagag gaugacgacu     1560

acagacaccu accuccuggc cgaagaugug cuucccaaac aaucccuguu guaucagcgu     1620

uucgaggucc ucaaugaacu gaacggaauc aagauagacg aucagccuau cacuguugag     1680

cagaagcaag caauuuuuaa ugacaaauuc augcagcagg cgcacgugac aguaaagaac     1740

auccaggcuu acuuaguguc agagggccaa uaugccaguc ggccaaauau uaccggccug     1800

agcgacgggg aaaaauuuaa cuccucucua agcacguacc acgaucucaa gaaaaucuuc     1860

ggagacauga uagaugaccc caauaagcag accgauuuug agaaaaugau ugaaugguca     1920

acaaucuucg aggacgguaa gauuuucgca gauaagcugc gagaaaucaa cuggcuuacu     1980

gacgagcaga gacagagguu gaguaauaaa cgcuaccggg gcugggggca acuguccacc     2040

aagcuccuga caaaaauagu cgaucagaac ggacagagaa ucauugacau cuuauggaac     2100

acuaaugaua acuuuaugag gauuauccau agcgacgauu ucgaccaagc cgugacccag     2160

gcuaaucaga ugcugcuugc cgacaacgau acacaggacg uuauaaauga guuguauacc     2220

ucuccgucaa acaagaaagc acuccgccaa auucugcuag uggucgcuga uauccagaag     2280

gccaugaagg gccaggcgcc ugcccgaauc cugauugaau uugcucggga gaaacaggca     2340

aauccccguc ucaguacuca aagaaggcgc cagguggaac agguauacca ggagcugaac     2400

aaugaccaac uuaacaagga auuggccgau ccacaguaug cuaaucugag caaagaccgg     2460

uuauuccucu acuuuaugca gggugggaga gauauguaua cgggcgaguc ccugaacauc     2520

gacaggcuuu cugaguacga uauugaccac auacugccuc aggccuucau caaggauaau     2580

ucguuggaca accgaguucu cgugagcgca aaaaugaauc gcuccaaguc agaccauguc     2640

ccccuggauu uuacagagga acgguucgga caaaagauga gaagucagug ggaacagaug     2700

cuaagggcgg gccugcucac ccgucgcaaa uauaacaacc ugacucuuaa uccagacucu     2760

uugaacaagu acaccaugag cggguuuauu aaucggcaac ugguggagac aagacagguc     2820

aucaaauuag ccaccaaccu ccugguggau caguacgcug acgaagaugu ucagcuugug     2880

acuguaaagu ccggacugac acaccaaaug agggaggacu ucgauuuucc caaaaaucga     2940

aaccuaaaua acuaucauca cgccuucgac gcauaccuca cggcuuuugu cggucuguau     3000

uugcugaaga aguacccuaa auuagcccac uauuucgugu acggccagua ucagcagggg     3060

uucaaggcaa augaucaaug gcggaacuuu aauuuccuca acggacugcg ccaggagcag     3120

cagguugacg ccgaaaccgg cgagcuuauu uggcaaaaag aucaugaccu ggcuuacaug     3180

aauaagaucu acgauuuuaa aaagauauug gugacuagag aaguccacac aaaccauggu     3240

gcgcucuaua aucagacccu guacaaagcc aaggacgaca aggcaucagg gcagggcgga     3300

aggcaacuaa ucgcugccaa agagaaccgg ccaacagcca uuuauggcgg guacagcgga     3360

aagacugugg cuuauaugug ccugguacga aucaccgaga aaaaggguga uuucuacaga     3420

auuuguggcg ugcagacggc auggguugac gaacuugagc acaucgaaga ugccaccaua     3480

aaacuccagc gccugacagc guugcugacu ccgcaguuuu cugcuaccaa gaagcaaaaa     3540

gacgaggucg ugacaguggu caagcccuuc gaaguuuuac ucccuaaaau ucugaucaac     3600

cagagguuuu augauggggg acaggagcuu acucuggccu ccgcaaccua caagaauaac     3660

gcccaggagu uggugcucga caaaaaggcu gugcaaguac ugaauggcca gcuaccaaag     3720

gcagaacugg aggcccaggc gacggucgug uacgaucaga uccuugaacg uguucaaaaa     3780

uauuuccccc ucuacgacau gaacaguuuu cgggagaagc uguucguggg uagagauaaa     3840

uuuaaugaau ugccuuggca ggaccagugg gagaaccaca aguuaauuca ggucgggcaa     3900

aagacaauac uggauaggau ccucauuggc cugcaugcua augccgcaau gggagaccuu     3960

aaggcccuga acaugagcac cccauucggc aaguugcaga aaucuaaugg gaucucccua     4020

ucacccgaga cucagauuau cuaucaaagu ccgacaggac uguucgaacg ccgaguggcu     4080

cucaaggauc uguga                                                      4095


<210>  3084
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3084
augaagaacc ugaaagagua cuauaucggc cucgacauug ggaccgccag cgugggaugg       60

gcugucacag augaauccua caauauaccc aaguucaacg guaaaaagau guggggcguu      120

agacuuuuug acgaugcaaa aacugccgag gaaaggcgga cgcagcgcgg gucucgacgu      180

agacugaaua ggcggaagga gcgcaucaac uuguuacaag accuguucgc gaccgaaauu      240

ucaaaggugg auccuaauuu cuuccuaaga cucgacaaca gugaucugua uagggaggac      300

aaagaugaga aacuuaaaag caaguacaca cuguuuaaug acaaagauuu caaggaccgg      360

gauuaucaca agaaauaccc aacuauccau cacuugauua uggaccucau cgaagacgag      420

ggaaagaaag auauacgccu gcuguaucuc gcuugccauu acuuacugaa gaaccgaggc      480

cacuuuaucu ucgaagguca gaaauuugac accaaaaauu ccuucgauaa gucuauuaac      540

gaccuuaaga uccauuugag agaugaguac aauauugacc uggaguuuaa caaugaagau      600

cuaaucgaga uaauuacaga caccacucuc aacaagacaa auaaaaagaa agaacugaag      660

aacaucguag gggauaccaa auuccuuaag gccauuucgg caaucaugau cggaucaagc      720

cagaagcugg uggacuuguu ugaggauggc gaauuugagg agacgacugu caaaagugug      780

gacuucucca ccacagccuu ugacgauaag uauucugaau acgaggaagc ucucggcgac      840

acuauuagcc uguuaaacau acugaaaucc aucuaugauu caaguauucu cgagaaucuu      900

cugaaggacg cagauaaaag caaggacggg aacaaguaca ucucuaaagc cuucguuaag      960

aaauuuaaua agcacggaaa agauuugaag acccuaaaga ggauaauuaa aaaguaucug     1020

ccguccgaau acgcuaacau cuuccggaau aaaucaauca acgacaauua uguggccuac     1080

acaaagagca acauuaccag uaauaaacgu acgaaggcgu cuaaauuuac uaagcaggag     1140

gauuucuaca aguuuaucaa aaagcaccuc gacacaauua aagagaccaa gcugaacucc     1200

agcgaaaaug aggaucuuaa acugauagac gaaauguuga cugauaucga guucaagaca     1260

uuuauuccca agcucaaauc gucugacaac ggugucaucc cuuaucaacu gaaguuaaug     1320

gaacugaaaa agauccuaga caaucagucc aaauacuaug auuuccucaa cgagucagac     1380

gaguacggca ccgugaagga uaagguagaa aguauuaugg aguuucgcau accauauuac     1440

guugggcccc ugaauccuga cagcaaauau gcauggauca agagagaaaa cacgaaaauu     1500

accccaugga acuucaagga uaucguggac cuugauuccu cuagggagga guuuauugac     1560

cgguugaucg gacgauguac auaccugaaa gaagagaagg uccuccccaa ggccagccug     1620

auauacaaug aguucauggu gcuuaacgag cugaauaacu ugaaacucaa ugaguuccug     1680

aucacugaag agaugaagaa agcuauuuuu gaagaguuau ucaagaccaa aaagaaagug     1740

acacugaagg ccgucucaaa ccuacucaag aaagaauuua aucugacugg cgauauccuu     1800

uugaguggua ccgacgggga uuucaagcag ggacugaacu ccuauauuga cuuuaaaaau     1860

aucauaggcg auaagguuga ccgcgacgau uacagaauua aaaucgagga aauuaucaag     1920

cucauagugc uguaugagga cgauaagaca uaccuuaaaa agaaaaucaa gagcgcauau     1980

aaaaacgacu ucaccgauga cgagauuaag aagaucgcug cccugaauua caaagauugg     2040

ggcaggcuuu cuaagcgguu uuugacuggg auugaaggag uagacaaaac gacaggugag     2100

aagggcucaa ucauauauuu caugcgcgaa uacaaccuca aucugaugga gcuaaugagu     2160

gggcauuaca ccuuuacuga agagguggag aaacugaacc cugucgaaaa uagagagcuc     2220

ugcuaugaaa ugguggauga gcuguaccuu agcccauccg uuaagaggau guuguggcag     2280

ucucugcgug uggucgacga gauuaagcgg aucaucggaa aagauccgaa gaaaauuuuc     2340

aucgaaaugg cgcgagccaa ggaggcaaag aacucgagaa aggaaagcag gaagaacaaa     2400

uuacucgagu uuuauaaguu cggcaaaaag gcuuuuauua augaaauagg ugaggagcgc     2460

uacaacuauc ugcuuaauga aaucaacucc gaggaagagu caaaauuccg gugggacaau     2520

cuguacuugu auuacaccca acucgggaga uguauguaca gucuggaacc cauugaccua     2580

gccgaucuga agucuaacaa uaucuaugac caggaucaca uuuacccuaa aagcaagauc     2640

uaugacgauu cccucgagaa cagggugcug guaaagaaaa aucuuaacca ugagaagggc     2700

aaucaguacc caauacccga aaaaguuuug aacaagaaug cuuauggauu cuggaaaauc     2760

cuguuugaca agggcuuaau ugggcagaag aaauacacac gccucacccg acggacuccu     2820

uucgaggaac gugagcuggc cgaauuuauc gagagacaaa uuguggagac aaggcaggca     2880

acgaaggaaa ccgccaaccu ucugaaaaau aucugccagg auucagagau agucuauagc     2940

aaggcugaaa acgcgucucg cuuccggcaa gaguuugaca uuaucaaaug uagaacugug     3000

aacgaucuac accacaugca ugacgcauac cucaauaucg ucgugggaaa cguuuacaau     3060

acaaaguuca ccaagaaccc acugaauuuu auuaaagaua aggacaacgu gagguccuau     3120

aauuuggaga acauguucaa auacgaugua guccgaggua guuauacagc cuggauagcu     3180

gacgacagcg aaggcaaugu gaaggccgca acuaucaaaa agguuaagcg cgagcuggaa     3240

gggaaaaacu accgguuuac cagaaugucu uauauuggaa cgggcgguuu auacgaucag     3300

aaucucauga ggaaggggaa aggccagauc ccccagaagg agaacaccaa uaaauccaac     3360

auugaaaagu auggaggcua caauaaagcc ucaagugcuu acuucgcccu gaucgagagc     3420

gacgggaagg caggacguga gcgcacacuu gaaacuauac cgaucauggu guauaaccaa     3480

gagaaguacg guaauaccga agcggucgau aaauaucuga aggacaacuu ggagcuccag     3540

gauccuaaaa uucugaagga caaaaucaag auuaacucgc uaaucaagcu ggauggcuuu     3600

cuuuacaaua uaaaagggaa gacaggagac ucccucucua uugcuggcag cgugcagcug     3660

aucguaaaca aagaagaaca gaaguugauc aaaaagaugg auaaguuccu ggugaaaaag     3720

aaagacaaua aggauauuaa aguuacuuca uucgacaaca ucaaggagga agaguuaaua     3780

aagcucuaua aaacccugag ugauaaacuu aauaacggua uuuacuccaa uaagcggaac     3840

aaucaagcca agaacaucuc ugaagcacug gacaaauuua aggagauuag caucgaagag     3900

aaaauagacg ucuugaauca gauuauccuc cuguuccagu ccuauaacaa ugggugcaac     3960

cuaaagucaa ucggccugag cgccaagacg ggaguggugu uuauucccaa aaaguuaaau     4020

uacaaagagu guaagcucau caaccagagu auuacaggcc uguucgaaaa cgaggucgau     4080

uuguuaaauc uguga                                                      4095


<210>  3085
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3085
auguacaaga aagacuauac cgugggccug gauaucggga gcacauccgu cggauucgcc       60

gcuauugacg augacuacca gcccauaaga cucaagggua aaaggguuau cggcgugcgg      120

cuuuuugagg aagggaagac ugcagccgag cgccgaaacu uccguacgac cagaaggcgg      180

cugaaucgca gaaaauggag guugucuuua cuggaagaga uuuuugaucc uuauauggcg      240

aagguagacc caacauucuu ugcucggcua aagcaaucaa accucuuccc gaaagauaag      300

aauaaaaagu uuacuggaag ucugcuuuuc cccaaaacca cagacagcaa guuuuacgau      360

gaauauccua ccaucuacca ucugcgcuau cacuugauga acgacucccg aaaguucgau      420

cucagagaga uuuuucuggc cauccaccau auagugaaau acaggggcaa uuuccugaac      480

ucugcaccaa ucgccucguu uucaacugac aagauuaauc ucgucgauga cuucgagaca      540

gugaacgcug cauacuuaca gaucaauagc aguaaccccg uucagcugga caccgaucag      600

gccgaccaag ugucccggau ucuuuuggau aauaaacugu cuaaccuaga caagcagaaa      660

caggcugcca aggcgcucgc acagaaaagc ggugauaaga agcaagacaa aaucaauaac      720

acgacuauaa agcagauuug caaagcccug cuuggguaua aguucgaucu ggcugucgug      780

uugaauaucg acuccgauga ccacaacaaa uggaagcucc agcugucaga ugaaaacauu      840

gacagucguu uagccgagcu gaccggagaa cucaaugagg cacagcuuga cauccugaac      900

guauugagcc gccuauacuc ucaaaucaca cugaaugaua uuguuccuuc cggcaugacu      960

cucucagagg cuaugauaga aaaguauaaa gcccauaaga aacaccugaa gaugcuuaaa     1020

gacuuugcga gaagccugag uaagaaggau uacggccagg ugauggaggc cuaugcagaa     1080

uacaucggga acauuccaga gucugcuucc agcaaagccc agaagaaaau cucgcaagac     1140

gauuucuaua agaaaauaaa gagguacuug gacgauucua agcucggaca gaaaauugcu     1200

gaacugaucg ccaagaauga guuuaugccc aaacagcgga ccaaccagaa uggugucauc     1260

ccuuaccaau uacaccugaa ggagcuaaac cagauuaucg aaaaucaguc caaauauuac     1320

ccauggcucg cagagcugaa cccgaaugaa aagcgacgcg gcaaagccaa guauaaacuu     1380

ucagaguugg uggcuuucag gauucccuac uauguggggc cucugauaac acaggaggag     1440

caacagaaaa ccaguaacgc gaauuuugca uggaugauca gaaagagcga gggacggauu     1500

acgccaugga acuucgacga aaaagucgau cgcaucgcca cugcuaauag auuuaucaag     1560

aggaugacaa ccaaagacac uuaccucauu ggcgaggaug uucugcccgc ccauucccuu     1620

cuguaugaac guuucaaggu guugaacgag cucaacauga uacggguaaa uaaccaccga     1680

cugucugugg gucagaagca ggacgucuuu gaaaaauuau uccaaaagca uaaaacagug     1740

aagaccagag agcugaucaa acagcuaaug gcaagcgggc ucccuacgca gccaaggauu     1800

accggacugu cagaucccgc caaguuuaau acaagucuug gcacuuacaa caauuucgcu     1860

aagaucuugg gcgacgagau uaaccagccg gaccgccaag aagauuuuga gaaaaucaua     1920

gaauggauca ccauuuucga cgauaaggac auccugaaaa caaagcucuc cgagcugagc     1980

ugguuagaug agaaacagau uaaugcccug aucaacaaga auuacucugg guggggacgg     2040

cuuucaagaa aguugcucac ugaacugacc aacaaaaagg gucagaguau aauugacagg     2100

auguggaaua cacaaaagag cuucaucgag auuaucaacg aaccuguuuu ugcagagcag     2160

auaaaggaug cgaaucagga ccagcuagau gaagacgauu augagaccgu gcugcacgac     2220

gcuuacacuu cuccccaaaa caaaaaggcc auccgccagg ucauuaaagu gguagaugac     2280

aucguucgag cagccggccg ggcuccaaag uucauuuccc ucgaguuugc ccgugaagac     2340

caggagucga gaaggacgca gagccgccug ucccggauca agaaaauaua ugaaacaacc     2400

acuaaggagc uuguggcaaa ugaucaaauu agagaagagu uggggaacgu caaagaccug     2460

aacgauaggu uauuccucua cuuuacccag cugggacgag acgcuuauac aggcgagcgc     2520

aucaauaucg augacauuuc aaccaaguac gauaucgacc acauuuuccc ucgggccuuu     2580

auaaaagaug acagucuuga uaacaaggug cuggucaaga gagcggugaa uaacgguaaa     2640

ucuaauaacg uucccuugaa acucuucggg gccaaaauga agccaaucug gaaaaugugg     2700

aaggaccauc agcugauuag caagaggaaa uaugaacacc uaaugacuaa ucccgacgcu     2760

aucgauaagu acaaagcaaa cggcuuuauu aaucgucagc ugaucgagac acgccaaaua     2820

aucaagcucg ccgcuaacau ucuggccaau cgguacucca cggaagacac caaaaucauu     2880

gaggugaagg caaaccuuaa ucaucaguug agacagucac ugcacuuaua uaagaacagg     2940

caaguaaaug auuaccauca cgcggucgac ggauaucuca gcgccuucgu gggccaguac     3000

cuguaucacc gauacccuuc ucuucagucc uauuuuguuu acgggcagua ccaacgguuc     3060

uuugauaaac aggugcagga aaacaugaag uucaaccgcu ucaauuuucu guaugaccua     3120

acugauagag acaacgauca gaucgucgug aaaaguacag gagagauagu aggugaaaag     3180

agcaaacuca uugaacaaau caagugggug uacagguaua aguucaugcu gguuucucag     3240

gaggucuuua ccaaaacagg cccauuguuc gaccagacua ucuacccggc uggguccaau     3300

aagucacggc ugauucccau aaagaaggau cgaccuaccg acaucuaugg aggcuacagu     3360

agcucgacgg augccuauau guccuuaauu agagugccag acaaaaagga agacaaauac     3420

aagguggucg guguucccuc ucgcagccuc gcaucacuga acagggcuga gaagcguggg     3480

caugaugccu accuugacca gcugcggcaa gugaucgcaa aaagucagaa gaaagaguuu     3540

cagauuauca uaccuaaguu gagauauagg cagcucauca uugauggcgg acugaaauac     3600

acccuaggcu ccucugccua ucaauacaau gcucgccagc uggugcuuag cgaaaagucc     3660

auggagguac ucgucacaga cuucucaaag gugacuaccg aucuggacaa acagaaccaa     3720

aaguugaucg cgguuuauga ugacauucug gauaaaguga aucgauguuu accacucuac     3780

gacaagaacc aguuuagaaa agcccugaau ggggcacggg auaaguucgu ccagcuuccc     3840

auccugccga aguuuaacgg aaauaaaaag gaagaggcug guaaagugga aauauugaac     3900

gagauucucc ugggccuaca cgacgaugcc agcacaggga aucugaagcc ucuuggaauc     3960

aaaacuccau ucggcaugcu ccagacccaa agugguauca cgaugucuuc caacgccguu     4020

auugugaauc agagccccac agggcuguuu gagcauagga ucuauuugaa ggcuuuacug     4080

aacaagcuca auaaauga                                                   4098


<210>  3086
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3086
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccauaggu       60

uucgccgugg ucgaugcuaa cucucaccuu cugagauuga aaggcgagac agcaaucggg      120

gccagguuau uucgggaagg acaaucagcg gcugaccgcc gaggcagucg uacuacgaga      180

aggcggcuga gccgcaccag auggaggcua uccuuccucc gggacuuuuu cgccccucau      240

auuacaaaga ucgauccaga cuuuuuccug cgccagaaau auucugagau uucgccgaag      300

gauaaagauc gauuuaagua cgaaaagaga cuuuucaaug acaggacuga ugcagaguuu      360

uaugaagacu accccucaau guaucaucug cgguugcacc ucaugaccca cacacauaaa      420

gccgauccuc gugagaucuu ccuggcuaua caccacaucc uuaagagccg cggucauuuu      480

cucaccccag gggcagccaa agacuucaac acugauaagg uugacuuaga ggacauuuuu      540

cccgcucuga cagaagccua cgcacaggug uauccugauc uugaguugac cuucgaccug      600

gcaaaagccg augacuucaa ggcuaagcua cucgaugaac aggccacgcc aagugacacu      660

caaaaagcac ugguaaaacu ucuguugucc ucugauggag agaaggagau cgugaagaaa      720

agaaagcagg uccucaccga auuugcuaaa gccauuacag gccugaagac uaaguucaau      780

uuagcgcugg gcaccgaggu ggacgaagcc gaugcaagca acuggcaguu uuccaugggg      840

cagcucgacg auaaaugguc aaauaucgag acaaguauga ccgaccaagg aacggaaaua      900

uucgagcaga uucaggagcu uuacagggcu cggcuguuga acgguaucgu ucccgccggc      960

augagccuau cucaggcuaa gguggccgac uacgggcaac acaaagaaga ucuggagcuc     1020

uuuaagacuu aucugaaaaa gcugaaugac caugaacugg caaagacaau ucgaggauug     1080

uacgaucgcu auaucaacgg cgacgaugcc aaaccuuucc ucagagagga cuuugucaag     1140

gcucugacca aagaagugac ugcgcaccca aaugagguau ccgagcaguu acugaacagg     1200

augggucagg caaauuucau gcuaaagcaa cggacaaaag ccaacggggc uaucccgguu     1260

cagcuccagc agcgcgaacu ggaucaaauu auagccaauc agucaaagua cuaugacugg     1320

cuugcaaagc ccaacccugu ggagaaacac agagccagca agccauacca guuggaugaa     1380

cugcucaccu uuaggauccc cuauuacguc ggaccucuga uuacgccaaa acagcaagcu     1440

gagaguggcg aaaacguguu cgccuggaug gugcguaaga aucccucugg caacaucacc     1500

ccguacaauu uugacgagaa agucgaucgg gaggcauccg cgaacacauu cauacagcga     1560

augaagacua ccgacacaua ucuuauugau gaagacguuc ugccuaagca gagcuugcuc     1620

uaccagaaau augaggugcu gaaugaauua aacaauguaa gaaucaacaa ugaggcucug     1680

ucgacugacc aaaagcagag gcuaauccau gagguguuug aacgccacuc uuccgucacc     1740

auuaaacagg ugguugauaa ccucguggcc cauggggacu ucgcacagcg gcccgagauc     1800

agaggacugg ccgaugaaaa gagguuccuu ucaaguuuga gcacauacca ccaacugaaa     1860

gagauucucc auggugcuau agacgauucc accaagcugc acgacaucga aaauauuauc     1920

acuuggucua cggucuuuga ggaugccgca aucuucaaaa caaaguuagc ugagauugac     1980

uggcuggauc cagccaccau aaagaaacuu agcaacaucc gcuaucgagg cugggggcag     2040

uuuucacgga aguugcucga cggacugaaa cuaggcaaug gucacacugu gauucaggaa     2100

cugaugcuca guaaccauaa ucugaugcaa auccuugcgg augagaccuu gaaggaaaca     2160

augaccgagc ugaaccagga caaauuaaag acugaugaca uugaagacgu aaucaacgau     2220

gccuacacau ccccuagcaa uaagaaagcu cuccgucagg uucugagagu ggucgaggac     2280

auaaagcacg cagccaacgg gcaggauccc ucuuggcuuu auaucgagac ggcugacggc     2340

accggaacug ccggcaaaag gacacaauca cgccagaagc agauucagac cguguacgca     2400

aaugcggccc aagaacugau cgauagugcu gucagagggg aguuggaaga caaaauugcc     2460

gauaaggcga gcuucacaga ccggcucgug cuguacuuua ugcagggagg uagggauauc     2520

uauacuggcg cuccacuaaa cauagaccag cugucccauu acgauauuga ccacauccuc     2580

ccccagucuc ugauuaagga cgauucgcuu gacaaucgag uuuuggugaa cgccaccauc     2640

aaucgcgaga aaaacaaugu auucgcaagc acgcuguuug ccuccaagau ggcugcgacc     2700

uggcggaaau ggcacgaagc cggguuaaua ucaggaagaa agcucaggaa ccugaugcuu     2760

cguccugaug agacagacaa auucgcacau ggcuuugucg cucgccaacu gguggagacu     2820

cggcagauca uuaagcuaac cgaacagauc gccgccgcuc aauauccaaa uacaaagauu     2880

aucgcaguua aagccggucu cagucaccag cugagagagg aauuggauuu cccgaagaac     2940

agggacguga aucauuacca ccaugcguuu gaugcuuucc uggccgcacg aauagggacu     3000

uauuuacuca aacgguaccc caagcuggcc ccauuuuuca ccuauggcga guucgcuaaa     3060

gucgacguga agaaauuucg cgaguucaac uuuauuggag cacuuacgca cgccaagaag     3120

aauaucaucg cgaaagauac aggcgaaauu guaugggaca aggagagaga uauccaggaa     3180

cuggacagga uuuacaacuu caaacggaug uugauaaccc acgaggugca uuuugaaacu     3240

gcugaucucu ucgaccagac aaucuacgcc gcaaaggacu cuaaagagcg agggggaaga     3300

augagccuga uuccuaagaa gcuagguuau cugaccggca ucuacggggg auaucaaacu     3360

gagcgcggcu ccuacguuuc aauuaucagg accuuuaaaa agggugaaac aauauauaaa     3420

gucgugaaca uccuuacggc cgaugugagc cuccugucug agaaggaaaa agucuccaag     3480

accuuggcug acauuuaccu gaaggaguua aucgccaaaa agcucaguaa aggcaagagc     3540

gggaaaaaua uugcaucuga acccuucgag aucguucugc cucguguggg aaaggauucc     3600

cuuuuuaaga acaaaaagua uggccuguuc uugauagagu cagcuacuga acuccacaau     3660

uaccaggagc uguggcuacc acggaacgac cagcugcuua uuaaaagaau ccaggccagg     3720

caaaucgaag agaguaaggc ggauuacgaa cucacacgca uguaucgaaa uauugccugg     3780

caaguggacc auuacuuuag acuguauaaa auacggaagu ucgcagagaa guugcaggcu     3840

agcguagagg auuucgccgg guuacccguc uacaaaaacg aaaccucgga cggaaaggug     3900

gagguucugg cuuccgugcu caaaaggcug aauggcgcag ccgauaaggg ucgcacaaac     3960

ccgacuaccg ucaaacuuca gucugacuuu gggcguacgc uguugacaaa uggaaucgug     4020

cuagaagaug acagcucaau uguuuaucag aguccuaccg gccuguucaa gagauccgug     4080

aaacucguag aucuguga                                                   4098


<210>  3087
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3087
augaccaagg aguacuaucu gggccucgac guggggacaa acagcgucgg augggccguu       60

acugauuccc aguacaaucu uugcaaauuc aagaaaaagg acaugugggg uaucagacug      120

uuugaaucug cuaacacggc aaaagauagg cgguugcaac gcggcaaucg acguagauua      180

gagaggaaga agcagcggau ugaccugcua caggaaauau ucucaccaga gaucuguaaa      240

auugauccua ccuuuuucau ccgccucaac gaaaguagac ugcaccuuga ggacaagagc      300

aaugauuuua aauauccacu guucauugag aaggacuacu ccgauaucga auauuacaaa      360

gaguuuccga caauauucca uuugaggaag caccucaucg aaucugagga gaagcaggac      420

auucggcuga ucuaucuggc ccuccauaac auuaucaaaa cucgcgggca cuuuuuaaua      480

gauggagacc ugcaaucggc gaagcagcuu cgacccauuu uggauaccuu ccugcuauca      540

cuccaggagg aacagaaucu gagcgugagu cuuuccgaaa accaaaaaga cgaguacgaa      600

gagauccuga agaauagauc uauugcuaaa agcgagaagg uaaagaaguu gaagaaccuc      660

uuugaaaucu ccgacgagcu ggaaaaagag gaaaagaaag cccagucagc agugaucgag      720

aauuucugca aguuuauugu cggcaacaaa ggugaugugu guaaguucuu aaggguuagu      780

aaggaggaac uggagauaga cagcuucucu uuuuccgaag ggaaauacga ggaugacauc      840

gugaagaauc ucgaagagaa agucccugag aagguguauc uuuucgaaca gaugaaagcc      900

auguacgauu ggaacauucu gguagacauc uuggagacag aagaguauau aucauuugcu      960

aagguuaagc aguacgagaa acauaagacc aaucuacggc ugcuccguga uauuauccug     1020

aaauauugca cuaaggacga auacaaccgc auguucaacg augagaaaga agcaggaagc     1080

uauacagccu acgugggcaa acuuaagaaa aauaacaaga aauacuggau cgagaagaaa     1140

agaaauccag aagaguuuua uaagagucug ggcaaauugc ucgacaagau ugagccccug     1200

aaggaagauu uagagguccu gaccaugaug aucgaagagu guaaaaacca cacgcuacuc     1260

ccuauucaaa agaauaaaga caacggggug auaccacacc aggugcauga agucgagcug     1320

aagaaaaucc uugagaaugc uaagaaguac uauucuuucu ugacugaaac cgacaaagau     1380

ggauacuccg uugugcagaa gauugagagc aucuuuaggu uccggauccc cuauuacgua     1440

gguccucugu cgacacgaca ccaagaaaaa ggcucuaacg uguggauggu ccgcaagcca     1500

gggagagagg acaggauuua uccguggaau auggaagaga uaaucgauuu ugagaaaucc     1560

aacgaaaauu ucauuacucg gaugaccaac aagugcacau accucaucgg agaggacgug     1620

cugcccaagc auucacuucu guacaguaaa uauaugguuu ugaaugaacu caacaaugug     1680

aagguccgcg gcaaaaagcu gccuaccagc uuaaaacaga agguguuuga ggaucuguuc     1740

gagaacaagu ccaaaguaac ggguaagaac cuacucgaau accugcagau ucaggacaaa     1800

gauauccaaa uagacgaucu uucuggguuu gacaaggauu ucaaaacuag cuugaaguca     1860

uaucuggacu ucaaaaagca gaucuuugga gaggaaauug agaaggaaag uauccagaau     1920

augauugagg auaucauaaa auggauuaca aucuacggca acgacaagga gaugcucaaa     1980

agaguuauua gggccaauua uuccaaccag cugaccgagg aacaaaugaa gaaaaucacu     2040

ggcuuccagu acagcgggug gggaaauuuu ucuaagaugu ucuuaaaggg uauaucaggc     2100

agugacguga gcacagggga aaccuuugau aucauuacgg cgauguggga gaccgacaac     2160

aaucugaugc agauccuuuc caaaaaguuc acauuuaugg auaacgucga agacuucaau     2220

ucuggaaaag ugggcaagau ugauaaaauc acuuaugacu cgaccgucaa ggagauguuu     2280

uugagcccag agaacaagcg ugcagugugg cagacaauac aaguugccga agagauuaaa     2340

aaggugaugg guugugaacc caaaaagauc uucaucgaga uggcucgagg gggcgaaaaa     2400

guaaagaagc ggacuaaauc cagaaaggcc cagcuccugg agcuauacgc agcuugcgag     2460

gaagauugua gggagcugau uaaagaaauc gaggaccgcg augagcggga cuuuaauuca     2520

augaaacucu uccuguacua uacccaguuu ggaaaaugca uguacagugg cgaugacauu     2580

gauauaaacg aacuuaucag aggaaauucu aagugggaca gggaccacau uuauccucag     2640

agcaagauca aagaugacuc cauugauaac uugguccugg ugaauaagac auacaacgcc     2700

aaaaagucaa acgaguuacu cagcgaagac auccaaaaga agaugcacuc uuucuggcug     2760

ucccuucuga auaaaaaguu gauaaccaag aguaaauaug aucgccucac ucgaaagggg     2820

gacuucacgg augaggaacu gagcgguuuu aucgcgcggc agcuaguuga gacacgucag     2880

ucuaccaaag ccauugcaga caucuucaag caaauuuacu ccucagaggu ggucuaugug     2940

aaaaguagcc ugguaucgga uuuuagaaag aagccacuca acuaccugaa auccaggcgc     3000

gugaaugacu accaucacgc uaaggaugcc uaucuuaaca ucguugucgg caauguguac     3060

aacaaaaagu ucacuucuaa ucccauacag uggaugaaaa agaaccggga caccaauuau     3120

agcuugaaca agguguuuga acaugacguc guuauuaaug gggaggugau cugggaaaaa     3180

uguacauacc acgaggauac caacacuuau gacggaggca cacuggauag aaucaggaag     3240

auuguggaac gagacaauau auuauacacg gaguaugcuu acugcgagaa aggugaacuc     3300

uucaacgcca ccauccagaa uaagaacggg aacucaacug uaagucugaa aaagggccuu     3360

gaugucaaga aauacggagg cuauuuuucc gcaaauacau cuuacuucag ccugauugag     3420

uuugaagaca agaaagggga ucgcgcccgg cauaucauug gagugccgau cuauauagcu     3480

aacaugcuag agcacucccc uucagcguuc cucgaauacu gcgagcagaa ggguuaucaa     3540

aauguuagaa uccuggugga gaaaauuaag aaaaacagcu ugcugaucau uaauggcuac     3600

cccuuaagga uccgugggga aaacgagguc gacaccaguu uuaagcgcgc aauacagcuc     3660

aagcuggauc agaaaaauua ugaacuugug cggaacauug agaaguuccu ggagaaauac     3720

guugaaaaga aaggaaauua cccaaucgac gagaacagag aucacaucac acaugaaaag     3780

augaaucagu uguaugaggu gcuccugucu aagaugaaaa aguucaacaa aaagggcaug     3840

gccgacccuu ccgauaggau ugagaaaagc aagcccaagu uuaucaaacu agaagaccug     3900

auagacaaga uuaauguaau caacaaaaug cuuaaucucc ugcgauguga uaacgacacu     3960

aaggcugauu ugucgcugau ugaguuaccc aaaaaugccg guucauucgu cgugaagaag     4020

aacaccaucg ggaaaucuaa gauaauucuc gugaaccaaa gugucacggg ccuguacgaa     4080

aaucggcgcg agcuuuga                                                   4098


<210>  3088
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3088
auggacaccg ccaagacaaa caaacagccc uacuauaucg gccuggauau ggggacuagc       60

uccgugggau gggcugucac ggacgcaucu uacaauguug ugaagaaaca cgguaaggcc      120

cucuggggcg uaagacuuuu cgaggaagcg caaaccgcug ccgauaggcg gcugcaucgc      180

acagcacgac guagaauaca gaggcggaaa cagcgcuugg ccuuacugca ggagcuauuu      240

gcuaaggaaa uuugcaaggu ggacccuggg uucuuuauca gacuccacga gucaaggcug      300

uggcaagaag auaaaaguau ugaccagcca aacacucuuu ucaaugauga ggacuuugau      360

gacaaggcau aucauaaagc cuacccgacc aucuaucauc ugcgguacgc uuugaugaca      420

gagaacaaga gcuucgaugu ccgccucgug uaucuggcca uucaccacau cauaaaacac      480

cgaggacauu uucugcucga aaauuacgac uuauccaacc aggagaccuc uggcuucgau      540

gaaucguaca ucgcgcugac uaaugcaauu aaggagcuuu ugcagaagga gauccccguu      600

ggucacgugg aaaagauuca aaucauacug aaggacaaau cacuaagcaa gagagagaaa      660

acagaaaaga uucuccaggu cugggacgug aucaaagaua agcagcugaa ggagguaguu      720

cagcuuauuu gugggggaac cgccaaccug aguacguugu uuauggacga aucccucaaa      780

gaggcucuga agguguucga gaucaaagau ucuaagauca gcuuuuccac ugccgucuau      840

gaagagaaag aaucagacuu acaaagugug cuggagcaga gguucgaucu cguguacgca      900

gcuaagaugc uuuaugacug gagccuguug gccgaacuaa ugggcgaucg gaagacccug      960

ucugaggcga aaauugccau auacgagaca cauaagaaag accucgaagu ccugaagcgu     1020

cuuacucgcc aggauaaauc ccuguauaga aaguuguuua ggggcaccga caaggauuca     1080

uacagcgcau auguugggag uugcaugauc cacggagaga aacucgugau ugaaaagcgg     1140

gguucuacag aggaguucua caaauuccug aagaaagaga ucuccaaguu accugacugu     1200

gaggacaaga uauacguacu gagcaaaauu gaagcugaga auuuucuacc aaaggccgug     1260

ucgaccauca acggcaucau ucccuaucaa cuccaggaac aggagcugga uaucauucuu     1320

aaaaaggcug agcaguacuu gccuuuccug auggaaaaag acgccuaugg gacgauaucu     1380

gagaagauca aacaacuccu gacuuuucgc auuccauucu acgucggacc ccuuaaucga     1440

cacuccgaua agucaugggc agugcauaca gacaaggaag gcagaauccu gccuuggaac     1500

uuuaaugaga ggaucaacga agagaaaagu gccgagaagu ucauuacccg gaugacuaau     1560

aaaugcacau auuugcucgg uaaggauguu cugccgaaaa acagcuuacu guacaccgaa     1620

uauaugcuau uuaacgagcu caauaacgug aagauaggga aggcuggaac guaccugacc     1680

gaagaacaga aagaaaaacu uuggaaagag uuguuccuga caacuaagaa agucacccuc     1740

uccaaguuuu cuaaguuccu gaucaaagag ggcauugaca aggaagaggc gguggaaauc     1800

aaaggcuuag augggggauu uaagagcuca cuggcaccau ggauugaccu uaaaaauauc     1860

uugggugagg auuucacacg cgacaaggcc gaagagauaa ucaaggcuau uacucucuuu     1920

aguauggaua aaaagauccu gaaaaaguac cuaaugagaa uuguauccga gcccguugcc     1980

aaacagcuga gcaagcucag guauaccggc ugggggcguu ucucuaaaga guuccugaca     2040

gaaaucaccc cucgggugac ugacgauguc caggugcuug ucgacacguc aacaggagaa     2100

auaaugaaca uuaucaccgc auugaagaag accagucuga auuuaaugga gauucucagc     2160

uccacuuacg gcuauucugc cgcuaucucg gaggccaaca aagaacuggu ggguaauaca     2220

guucuuagcu acgagaccgu gaaggaacug acuguauccc cagcagucaa acgacccaua     2280

uggcaaacau ugaagaucau uaaagagauc acgcacauua ugggggcgga accugcuaga     2340

aucuuuauag agauggccag ggacaagcag ccagauaagg gccgcaccga cucacggaaa     2400

aagcagcuca uugagcugua uaaaaagugu aaagaagaug ugagaaguug gucuaacagg     2460

gagagcaagg acuggguuga agagaucgaa uccaagucag auagccagcu acgcucuaau     2520

aaacuguucc ucuacuauac ucaaauggga aagugcaugu acacaggcaa acccaucgac     2580

cugggggcac uuuugaaggg acagcuguac gaucgagacc auauuuaucc gcagucccag     2640

accaaagaug acaguuuaga uaaccucgug aucgucgaca aucgggagaa ccgugauaag     2700

agcaauacau acccucuguc uaagaacauu agagagaggc aaauagguuu uuggaaacuu     2760

cugcgcgaca agggcuucau cuccaaagaa aaguauuuuc ggcuaacuag aaauaccccc     2820

uucucagacg ccgagaaagc ugaauuuauu gccaggcagc ucguggagac gcgacagagu     2880

accaaggcag uagcugaaau ccugaagcgc guguugccaa aaagcauuau cguuuacguc     2940

aaggccgggc ugacaucgcg guucagacaa uauggagagu uuauaaaagu gagggauuua     3000

aacgacuacc accaugcgaa ggaugccuau cucaacaucg ugacuggcaa uguccacuac     3060

accaaauuca cauccaaccc uauucguuuu guuaaggacu gucagaucua cucucugaau     3120

gagcacgcuc uuuauaaaua cacuguggaa cgcaacggug ugguagcaug ggcccccaau     3180

gaggaaggga ugaugccaag cgucacccgg uggaugaaca agaauaacau ucuggugacg     3240

agaaugucau auguuggcca gggagaguug uucgaucagc ucccccugaa gaaaggcaag     3300

gggcaaguga gucugaaaaa ggagggagcu aucuccgaca uaucuaaaua cgguggcuau     3360

aauagcgccu ccacagcaua cuuuauguau gucgaagggg aggauaacaa gggaaagccu     3420

guguacguua uugaaaccau gccacuacau cuugcggcca ggcucacuac acuggaggaa     3480

aaacagaagu acugcgagga guuguggaug ucaaccggcg guaaacugaa gaauccgaaa     3540

guguuaaucg aacagauccc cauucagagc cucuucagug uagacggguu ccgagucacu     3600

auagcuggca agaccggaaa gcaauauggc ugucggcugg ccgaggaacu ugugcugaac     3660

aauggguacg caaugacauu gaaagcugug cucaaguuug ucaaccgcag aaaaaugaau     3720

aaggaggccc ugaucacguc uuaugaucac cuagaagagu cccagcugcu ugcacucuac     3780

gacguuuucc uggauaaauu ggagcauagc ugcuuuucga agagguucuc aacccagcgg     3840

gacguguuaa agaacggacg agaaucuuau auugcccuga gucucgagga caaaugucag     3900

auccuguccg aaauucuuca ccuguuucaa ugcaacgcua cuuugagcaa ucuaucucug     3960

cucggcagcu ccaagcaguc agguagacug uacaugagua aaaaccuuag cgauaaggac     4020

ucuugguaua ucauacacca guccguaaca ggguucuacg agcaacauau cccuuuagcg     4080

ccauacaaaa agucauga                                                   4098


<210>  3089
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3089
auggugaaga aaaaguacgc caucggcauu gacauaggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa aguacccucu aagaaaauga agguguucgg uaauacugag      120

aagagauaca ucaaaaagaa ccugcucggc acgcuucugu uugaugaagg gaauaccgcu      180

gagaacaggc gguugaaacg cacagcacga cguagauaua cuaggcggcg caauagaauu      240

uuauaccugc aggaaaucuu cgccgaggaa auuaacaaga ucgacgauuc auuuuuccaa      300

aggcuagacg auaguuuucu cauagucgag gacaaacagg gaagcaagca cccuaucuuc      360

ggcacccugc aggaggaaaa gaaauaucau aagcaguuuc caacaauuua ccaccuucgg      420

aaacaacugg cggauuccuc ucagaaggcu gacauccgcu ugauuuaucu cgcccuggca      480

cauaucauaa aauaccgagg ucacuuccug uuugaggggg aucucaaguc ggaaaauaaa      540

gacgugcagc auuuauucaa cgauuuuguu gagauguucg acaagaccgu ggagggauca      600

uaccugagcg aaaaucuucc gaacgucgcc gacguguugg uagagaaggu uaguaaaucc      660

agaaggcugg aaaauauucu acacuauuuu cccaacgaga agaaaaaugg ccucuucggc      720

aacuuccugg cucuugcacu gggguugcag ccuaauuuua agacuaacuu cgaacucgcc      780

gaggaugcua aaauccaauu uucuaaggag acauacgaag aggaccugga agaguuacug      840

ggaaagauug gugaugacua ugccgaucuc uucaucgcga ccaaaagccu uuacgacggc      900

auccuguugg cagggauucu auccacgacu gauucaacca caaaggcccc acugaguagc      960

ucuaugguga accgguauga ggaacacaaa aaggaccucg cucugcuuaa aaauuuuaua     1020

caucagaacc uguccgauuc auacaaggag gucuucaaug acaaguugaa agauggauau     1080

gccggcuaca ucgaagguaa gacuacccag gagaacuuuu accguuucau uaaaaaggca     1140

aucgaaaaga uagaggggag cgacuauuuu auugacaaga ucgaucgcga agacuuccuc     1200

agaaagcaga ggacauuuga uaauggaagu aucccccacc aaauucaucu gcaggagaug     1260

cacgcuauca uucggcgaca ggccgaguuc uacccuuucu uaguggaaaa ccaagacaaa     1320

auagagaaga uccugaccuu ccgcauucca uauuacgugg gcccccuagc gagaggcaaa     1380

ucugaauuug ccuggcucaa uaggaagucc gaugagaaaa uccggccuug gaacuuugac     1440

gagauggucg auaaggaaac gagcgcagag aauuucauca cucgcaugac aaacuaugac     1500

caguaccugc cagaucagaa aguucuuccg aagcacucgu ugcuguauga aaaguuugcu     1560

guguacaaug agcucaccaa aguaaaguuc auugccgaag ggaugagaga cuaccaguuu     1620

cuggauucug gacagaaaaa ggacauagug aaaacucuuu ucaagacaaa gaggaaaguc     1680

accgcuaagg auaucaaagc cuaucuggag aacuccaaug guuacgcagg cguggaguug     1740

aaggggcucg aggaacaauu uaacgccuca cugcccacgu aucaugacuu acugaaaauu     1800

cuacgugaca aggcuuucau cgaugcggaa gagaaucagg aaauucucga ggacaucguu     1860

cugacccuua cauuguuuga ggaucgggaa augauacgaa agagacugga gaaauacaag     1920

gacauccuca cugaggaaca gaggaaaaag cuggaacgcc ggcacuauac cggauggggc     1980

agauuaagug caaaacugau uaacgguauc cuugauaagg ugacaaggaa gacuauuuug     2040

ggguaccuca ucgacgaugg caccagcaac cgcaauuuca ugcaacugau aaacgacgau     2100

acacuauccu uugucgacga gauucgacug gcccagggau cuggcgaggc ugaagauuau     2160

cgggccgagg ugcagaaucu cgcagggagc ccugccauca aaaaggguau ucugcaguca     2220

cuuaaaaucg uagacgaauu gauagagguu augggauacg auccagagca uaucgugguc     2280

gaaauggcuc gugagaacca auucaccaau cagggcagaa ggaacaguca gcagcgcuac     2340

aagaaaauug aaaaugccau caagaaccug gacuccaauu uaaacagcaa aauucucaag     2400

gaguauccca cuaauaacca agcacugcag aaugaccggc uuuuucugua cuauuugcag     2460

aacgggaagg auauguacac ggacgaggaa cucgauaucg accaacuguc ucaguaugau     2520

auagaccaca uuaucccuca ggcguucauc aaagaugacu cacuagauaa uaaggugcug     2580

acaaaaagug cuaagaacag aggaaaaagc gacgaugucc caucccucga gauugugcau     2640

aagaagaaaa acuucuggaa gcagcugcuu gacucucaau ugaucucgca gaggaaauuu     2700

gacaaucuga ccaaggccga acgaggcggu uuaacuaacg aggauaaagc acgcuucauu     2760

cagcggcagc ucguugaaac cagacaaaua acaaagcacg uggccaggau ccuggacacc     2820

cguuuuaaua cuaaacuuga ugaggcuggg aaccgcauuc gggaccccaa aguaaauauc     2880

auuacacuca agagcaaccu agucucccag uucagaaaag auuaccagcu cuauaaggug     2940

agggaaauca auaacuacca ccaugcccac gacgcauacc ugaaugcugu uguggccacg     3000

gcguugcuga aaaaguaucc gcaguuagcc ccugaguuug ucuacggcga uuaucccaag     3060

uacaacucau auaaaagucg aaagucugcu accgagaaag ugcuguucua cagcaauaua     3120

augaacuuuu uccggcgcgu acugguguau uccaagacug gagaaguuag aaucaggcca     3180

gucauugagg ugaauaaaga aacaggcgag aucguguggg acaagaaguc agauuuucgg     3240

accguccgaa aaguucuuag cuacccucaa gugaacgugg uaaagaaagu cgaaaugcag     3300

acagggggau ucucuaagga guccauucug cagcauggug acagugauaa auugaucccc     3360

agaaagacug agaaauuuua ccucgacacc aagaaguaug gcggguucga uagcccaacg     3420

auagcauacu cugugcugcu aauugccgac aucgaaaaag gaaaggcuaa aaagcugaaa     3480

cgcguuaagg agcuuaucgg cauuaccaua auggaaagga uggccuucga gaagaauccc     3540

aucgaguuuc ucgaacacaa agguuauaag aacauucugg agaaaaacau cauuaaguug     3600

ccuaaauacu cccuguucga auuagagaau gggcgucgga gacuccuggc aucagcgaag     3660

gaacuucaaa agggcaacga gaugauccug ccaccgcacu uggugacacu ccuguaucau     3720

aguagcaaua uacacaaaau cacugagccc auucaucuaa acuacgucaa uaagaacaaa     3780

cacgaauuua aggagcugcu uaggcauauc ucggacuucu ccacccgcua uauucucgcc     3840

caggaucgac ugucuaaaau cgaagaguug uacgacaaga augauggaga cgauauaagc     3900

gacuuaacau caaguuuugu gaaccugcuc acuuucaccg cuauuggcgc cccugcagcu     3960

uuuaaguucc ugggguccgu uaucgauaga aaacgguaca cgucuaucgc cgaaauucuu     4020

gaggcaacac ugauccacca gagcgugacc ggauuguaug agacuaggau agaccuaucc     4080

aagcugggcg gugauuga                                                   4098


<210>  3090
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3090
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

auaacagaug acuauaaggu uccugcuaaa aagaugaaag ugcucgguaa uacuaacaag      120

aagacgauca aaaagaaucu uauuggcgca cuguuguucg augccgggaa caccgcggcu      180

gacagaaggu uaaaacggac agcccgccga cguuacacua gaaggcggaa ucgcauccug      240

uaucuacagg agauuuuugc agccgaaaug aacaagcucg augagucuuu cuuucacaga      300

cuggacgauu cauuccuugu accagaagac aaaggaggca guaaguaccc gaucuuuggu      360

acccuggagg aagagaagga guaucauaaa caauucccca caauauacca cuugagaaag      420

agccucgcug auuccaaaga aaaggcagac cugcggcuga ucuaucucgc cuuagcucau      480

auuaucaaau accgcgggca cuuucuguac gaggauaccu ucgacauuaa gaauaacgau      540

auccagaaaa uauuuaauga guucacuucu auuuauaaca auaucuuuga agagucguca      600

cuuagcaacg aaaaugccca gguggaggaa auuuucacag acaagaucag uaaguccgcg      660

aaaaaggacc gagucuugaa acuguuuccu gaugagaagu cuaccggacu auucagcgag      720

uuccucaaac ugaucguggg caacaaggca gacuuuaaga aacauuucga ucuugaagag      780

auggccccac ugcaguuuuc caaggacacg uacgaugaag acuuggaguc acuccugggc      840

caaauugggg augacuaugc ugauuuauuc guuguggcca aaaagcugua cgacgcaaua      900

cuccuugcug gaauccugag ugucaaagau cccgccacua aggcgccuuu gagcgccucu      960

augauugaaa gauaugacaa ucaccagaac gaccuauccg cacugaagca guuuaucagg     1020

ucaaaccucc cagagaaaua cgcugaagug uucagcgaug acaguaagga ugguuaugcc     1080

ggcuacauag acgggaaaac cacacaggag ggauuuuaca aguauauuaa aaaucaaauc     1140

ucuaaguucg agggcacuaa guacuuucug gagaaaaucg aacgggaaga uuuccuucgu     1200

aagcagcgca ccuuugacaa cgguuccauu ccccaccaga uccaucugca ggagaugaau     1260

gcuauuuugc aaaaccaagg gggauauuac ccuuuccuca aagccaauaa ggaaagaaua     1320

gagcagaucc ugacauuuag gauuccauau uacguaggcc cguuagcacg gggcaacagc     1380

gauuucgccu ggcugacccg aaauucggac gaugcuaucc gccccuggaa cuucgaggaa     1440

guuguggaca aagcgucuuc cgcagaggau uuuaucaaua agaugacgaa cuaugaccua     1500

uaccucccug aagagaaagu ccugccaaag cacucacuuu uguacgagac uuucgccgug     1560

uauaaugaac ugacaaaggu gaaauuuauu gcugaggcca ugaaggauua cgaguuccuc     1620

gacagugggc agaaaaggaa gauagucgaa aacaucuuua aaaaucauag gaagguuacc     1680

gaaaaggaca uuaucaacua ucugcacaau guggauggau acgacgguau ugagaucaaa     1740

ggcauagaga agcaguucaa cgcaagccuu uccacuuauc acgaucuguu gaaaaucauu     1800

aaggacaaag aauuuaugga uaaccccaag aauggggaga uccucgaauc uauuauccau     1860

acacugacca uauucgagga ccgggaaaug auuaagcagu uacuggccca guacaacagc     1920

guauuugaug agaaagugau caaggcucua ucacgcaaac acuauacggg auggggcaag     1980

cucagugcca aacugauuaa ugguaucaga gacaagcaaa ccgggaacuc cauacuugau     2040

uacuugaugg acgauggcaa gagcaauagg aacuucaugc agcugaucaa agacgaugga     2100

cucucuuuua aggagauuau cgacaaagca caggucgugg gcgaaacaga caaucugcgu     2160

cagguugugc aaaacuuacc ugggucacca gcgauuaaga aaggaauccu gcagaguaua     2220

aaacuugucg augaguuggu gaaaguaaug gguagcgacc ccgaauccau uguuaucgag     2280

auggcucggg aaaaucagac uaccaacaag ggcaagucua aaucgcagca acgacucaag     2340

acacugagcg augccaucuc cgagcuaggg ucaaauauuc ugaaagagca uccgacugac     2400

aacauccagc uccagaauga uagacuguuc cuuuacuauu ugcagaacgg aaaggacaug     2460

uacaccggcg aagcacugga uauuaaucaa uuaaguaacu augacauaga ucacaucauu     2520

ccucaggccu uuaucaaaga cgauucucuc gacaacagcg ugcugacauc cucaaaggac     2580

aauaggggua agagcgauaa cguccccucu cuugagauug uggagaaaau gaagggguuc     2640

uggcagcaac uguuggacuc caaacucauc agugaacgca aguucaauaa ccugaccaaa     2700

gucgagcggg gcggacuaga ugaaagagac aaggugggcu uuauaaagag gcagcugguu     2760

gagacucgcc agaucacgaa acauguggcu cagauucucg augcccgauu caaugcagaa     2820

guaaacgaga agaaucaaaa acugcggagc gucaagauca uuacacuuaa aucuaacuug     2880

guguccaauu uucguaagga guucgggcug uacaagguua gagaaaucaa cgacuaucac     2940

cacgcucaug augccuacuu aaaugcggug auagccaaag cuauucucaa ggaguaucca     3000

aaacuggaac cugaguuugu cuacggagac uaccagaagu augaucuuaa aagguacauc     3060

ucacgcacca aggaccccaa ggaaaucgag aaagcaacug aaaaguauuu cuuuuacagu     3120

aaccugcuaa auuucuuuaa agauaaggug uauuacgccg acgguaccau uauacagcgg     3180

ggcaacguag aguauagcaa agauacaggg gagaucgcuu ggaauaagaa aagagacuuc     3240

gccauuguga ggaagguucu cucguaccca caggucaaca ucgugaagaa aaccgaggaa     3300

caaacuggag gcuuuuccaa ggaaucuauu cugcccaaag guaacagcga caaguugauc     3360

ccucgaaaaa caaagaauau acagcuggau acgaccaagu acgggggcuu cgacucacca     3420

gugaucgcau auaguauuuu acucgucgcg gauguugaga aaggaaaguc caaaaagcug     3480

aaaacuguga agucucuuau cggcauuaca aucauggaaa aggugaaauu cgaggccaac     3540

ccgguagcuu uucuggaggg gaagggauac cagaaugucg uggaagagaa cauaauucgc     3600

uugcccaaau auagccucuu cgaacuggag aauggccgga gaaggaugcu agccuccuca     3660

aaggagcugc agaaagguaa cgaaaugguu cuuccuagcu accuccuggc auugcuguau     3720

cacgcuaaga agauccaaaa gaaggacgag ccagaacauu uagaguacau caaacagcac     3780

cacagugaau uuaaugaucu ccugaacuuc gugucugacu uuucccagaa guauguccuu     3840

gccgagagca aucuggagaa aauuaagaac uuguacaucg auaaugaaca aaccaaccuc     3900

gaggaaauag caaauucguu cauuaaccug cuaacauuua cugccuucgg ggcucccgcg     3960

guguuuaaau ucuuuggaaa ggacaucgag cguaagcgcu acucaaccgu uacggaaauu     4020

cugaaagcca cccuuaucca ucagucuaua acaggccucu augagacucg gauugaucug     4080

aguaaguugg gugaggaaug a                                               4101


<210>  3091
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3091
auggacaaga aauacaacau cggccuggau auugggacca gcuccauagg auucguggcc       60

acagacgagg auaaucacgu caucagagcu aaggguaaaa acguuauugg cgugaggcuc      120

uuugacgaag ggcagucugc agccgagcgg cgcggauucc gaacuacgcg uagaaggcuu      180

aaucggcgca aguggagacu gaacuuguua agggaaaucu uugagcccca uauuuauccu      240

aucgaugcgu cauucuuuau gcggcaaaaa uacaguaauc ugagcaagca ggacccaaac      300

cagaagauag aguucaaaga uauccuauuu uccgacaagu cugaugcuga guucuaucgc      360

caguacccga ccauuuauca ccuccgacaa gcccugauga cagagaaaag acaguuugac      420

cuuagggaaa ucuaccuggc aauucaucac aucguaaagu aucggggcca uuucuuggau      480

gagacuccca ccgacaaauu uucguucgau cagcucgacc ugacacagga auuugagcgu      540

cugaaugucc ucuucucaga gcaaaacccu gaagaaccau ucgaaauagu ggugaaucag      600

aaccagagca uugaggacau cuuaaguaau caguccauga ccgccucuga ucgcagaaag      660

caagcucugg uuguguuuua ccagcagagc gaaaacaagg agaaugagaa acaauacaag      720

gcaauugcca cugaaauccu uaacuugcug cuagguaaua aagcuaagcu caacgucauc      780

cuggcccagg agguggacaa agaagcggca aaggauuggu cccuuucacu gaguagcgag      840

acauucgacg augacuugga aaaagccucu uccgggcuca acgauucaca gcuggagaug      900

guaggaauuu uugagaggau auauagcagu uuaauccuga agucuauuau cggcgaccac      960

aauucccuca gcgcugccau gguugauaag uucaaaaccc acgcacagca ucgggaccuu     1020

cugaaggaug ugauaaagaa cucggacgcu acgaagggcg aacaauugca guuugccuac     1080

gaccacuauc uacagcgaca uucugcggau gggaaagccu acggaaagga cgauuucuau     1140

aaggagguca aaaagaaucu ggacgauucc gacgcagcuc agcaaauuca gcagcucauc     1200

gauacugacc aguuuaugcc caaacaacgc accaaggcca acggugcuau cccucaccag     1260

gugcagcaga aagaacugga ucuuauuauc gagaaucaau gccaguacua uccauggcug     1320

gccgaagaga accccaauuc agagagacac aacguggcaa aguacaaguu ggacgaacuc     1380

gucaguuuca ggguuccuua cuaugugggc ccacugauua gcccggccga ugacgagaca     1440

gauccccagg cuauacaaaa uacuaaauuu gcguggauga uccggaagga cgcacagggg     1500

cgcauuaccc cuuggaacuu cgacgauaaa guagacagag aaaaguccgc cgauaaauuu     1560

aucaagagga ugacaaccac ggacacuuac uuaaucggag aggaugugcu gccagaccag     1620

ucucuaauuu aucagcguua caagguccuc aaugagcuga acaaugugcg gauaaacggc     1680

cgacccaucg aaguucaaau uaaacagaau aucuauacag aucuuuucaa gaaauuuaag     1740

agcgugucag ucaaaaaguu gagaaguuac cuggaggaaa uguccuauua cccugguagc     1800

uucaaaauuu cugggcucac caacccagga aaguucgaca acggccugac uucauaccuu     1860

gauuauaaga auauccuggg cgacagugug gaugcuaacg acaaaagggc cgauauagag     1920

cagaugaucg aauggagcac aauuuuugag gacaauaaga ucuucgcaca gaaauuggag     1980

uccaugaccu ggcucacggc cgaacaacgc aaggcuauuu cucggaaaag auacaccggg     2040

uggggaaggc ugucgaacaa guuacugaca cagcuaaaga aucaggacaa ccgcagcauc     2100

auggaucucc ugugggagac ugaagagaau uuuaugaaaa uacaguccgc ccccgacuuc     2160

ucaaagauga uugaagcaca uaaccaacag uuuaucaaaa augaggcgaa cauucagcaa     2220

guaaucagug aucuuuauac cucuccgcag aauaagaaag cuauacgaca gaucuugcug     2280

guuguggacg auauucagaa ggccgucggu cacgcaccug agcauaucgc cauugaguuc     2340

gcuaagggcg aggacaaaaa cccccggcgu acaaucagca gagaaaugaa gauacaagau     2400

guguaccagc agauuuccga cgauauccuc gucucaccag aggugagggc ccagcugaau     2460

gagaaaaucg aaaaccgcca cgggcgguua agcgacagac uguuucuuua uuucaugcaa     2520

ggaggcaggg auauguacac ugguaagccu auuaauauca acaacauuuc ucauuaugac     2580

auagaccaca ucuugcccca ggcauuuauu aaagaugacu cccucgauaa ucgaguucug     2640

gugagugaca agcuaaaccg cgaaaagagc gauaccguac cacgggaccu guucgggaga     2700

gaaaugaugu cuacauggga gaaaauggcu gauaggggcc ucaucaccaa gcguaaacug     2760

gccaaucuua agacuauucc cggauccauc gacgcgcaca aagcccaggg cuuuauaaac     2820

cgccaguugg ucgaaacgcg gcaagugauc aagcuggcug uugggauuau gaucgaucag     2880

uacccugaga cacagauugu gucagucaaa gcaaauuuaa cccaugagau gagagaaaug     2940

uucgaccucg ugaagaacag ggauguaaau gacuaccacc augccuuuga ugcuuaucug     3000

acugccuucg ugggaaacua ccuucugcag cgauauccaa aguuggagcc guacuucguu     3060

uaugacaaau uuaagaaacu caaggaccaa aauaucagua ugcgggauuu caaccugcua     3120

uacgaccuga aaaagaauga agaugucuau gaccagcgca ccggucacag cgcacacaag     3180

auaugggauc agcauggcuc gauugacuac aucagacacc ucuuugcgua caaaaagauc     3240

cugguguccc augaggugca ccaaaacggg ggagcccuuu auaauaugac agucuacggc     3300

aaagcugaug acgccaagag gaaauugauu cccauaaagg cagauaagcc uaccgaacug     3360

uauggugggu acacuucucg gaaauuagcu uauauggcca ucguuaagac gaaaaagggc     3420

aaaaaggagc aguaccaggu gacaggagug ccaguaagcg cacucaccac ucugcgacag     3480

uucaaggagg acucacccga acaaaaacag gcccuugagg aaauugucag acgcaacuuu     3540

gaugagaaga cagaagugga gaucauuguu ccuaaagugc uguucaauca gcuaaucguc     3600

gacgagaagu guagguuuau ggugggcagu gauaccuauc guuacaacgc ucagcaacuc     3660

guucuguccg cggacgacuu ggaaauaauc aacgccucuc auaaagauau ucagacaauc     3720

gacagcuccg auuacucaca gcagcuggac caaguguauc agaauauuuu aacuaucgua     3780

gauaagcacu ucccaaugua cgacauaaac aaguuucgga ccaaacucca cgauauuuau     3840

gagcaguaca ucgaacugcc caaugaggac gucugggaaa acaauaaacu uacgacccug     3900

gggaaacaua caaucauuau cagaauauug caaggacucc acgcaaacgc uacuguggug     3960

gaucugaagc agcuagguau uaaaaccccg uucggcuuua ugcagcaggc cagggggauc     4020

gucguuagcc cugacgccca acugauuuau cagaguccaa caggacuuua cgagcgccga     4080

gugagacucg gcgaucugug a                                               4101


<210>  3092
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3092
augggggaca gaaaguacaa ccuggggcuc gauaucggaa ccagcuccau ugguuucgcc       60

gcuguggacg agaauaacca gcccauaagg gucaaaggca agacagcaau cgggguucgg      120

cuuuuugaag agggaaaaac ugccgcggau cgccgaggcu uccguacgac cagaaggcgg      180

cugucucgca gaagguggcg gauuaauuug uuaaacggua ucuuugacgc ucaccuggcc      240

gaaguggauc cuacauuccu agcacgccuc aaggagucaa aucgaaguaa ccuggacccg      300

aaaaagagcu uucaaggguc ccuucuguuc ccagaaagaa aggauuauca guuuuacaaa      360

gaguauccca cuauuuacca uuugaggaag gcccucaugg aggaagaccg gaaauucgau      420

auccgugaga uauaucuggc uguacaccau aucauuaagu accgcggaaa uuuucugaac      480

ggcaccccua ugagaucuuu caaaguggaa aauaucgagc ucgacacauu auuugauagg      540

cugaaccagc uuuaugagga aauuauccca gacaaugagu uggcauucga ucuggaacag      600

gucgccgacg ugaaggaggu ucuaucguca accacuauau acaagaugga caaaaagaaa      660

caacucguga agaugaugcu gcuucccaca agcaacaaag cucugcagag ugaaaauaag      720

aaaauuguca cccaguuugu gaacgccauc uugaauuaca aguucaagcu cgauguacug      780

uuacagguug agacggacgc ggauuggucc cugaaacuca acgacgaggg cgcagaugac      840

aagcuggaag aguucacugg ggaucuggac gaaaaccggu uggagauuau cgaucuacug      900

caacgacucc acaauugguu uucucugaac gaaaucacca aagacggaaa uagccuuucc      960

gccgcuaugg uggagaagua uaaagaucau cgccaccacc uggaguugcu caagaagguc     1020

auugaagacc auccugacgc caaaaaggca aaagcucuga aggagacaua cacugccuau     1080

guggguaaaa ccgaugacaa gacacagaac caggaugacu ucuacaaggc gguggaaaag     1140

aauuuagaug acucaccaga ugccaaggag auaaaaagac ugauccagcu agaccaauuu     1200

augcccaagc agaggaccgg ccagaacggg gcaauuccuc accaacucca ucagcaggaa     1260

cuggaucaga ucauagagaa acaaaguaag uauuacccau uccuugcuga gccgaauccc     1320

aacgucaagc ggcgcaaaga cgccccuuau aaguuggaug aacugauugc uuuuaaaauc     1380

ccauacuacg uuggaccccu cgugacgccu gaggaacagg cccagaaugg cgagaacgua     1440

uucgcaugga ugaagagaaa aguggccggu ccaaucacuc ccuggaauuu ugacaagaaa     1500

gucgauagga uggagagcgc uaaccguuuc auucggcgaa ugacaaccaa ggacacuuau     1560

cuguuugggg aagacgugcu uccggcggag ucuaugaucu acgaaaaguu cguugugcug     1620

aaugaguuga acaaucucaa aauuaacgga agacaccugu ccuuaaagga uaaacaggac     1680

auauauaacg aucuguuuaa gcaacagaaa acagucucaa ucaaggcacu acagaauuac     1740

uaugugacca agaaaaaggc cgcuacggcc ccuaccguag gcggccucgc agaccccaaa     1800

aaguuccuga gcagucuuuc uacauacauu gauuuuaaaa acauguucgg ggaacagguu     1860

aaugacccac aauuccagga ggauuuggag cagaucgugg aaugguccac uaucuuugag     1920

gacaggggaa uuuucaaggc caagcugcag gcucucgguu ggcugagcga aaaacaaaua     1980

cagcaguuag ucgccaagcg cuauaaaggc ugggggcggc ugucgaagaa acuuuugacc     2040

ggacucaaga acgcagaggg cuacucuauc cuggaugaaa uguggagauc cacagagaau     2100

uuuaugcaaa uucagucaag gccugaguuc gcggcucuaa uccagcaggc caacgaaaag     2160

caauuugagg guaaugaccc caacaaugug ugggaaaaca uugagaauau ccugggggau     2220

gcauacacca guccacagaa caaaaaggcc auacgccagg ucgugaaagu ugugcaggac     2280

aucgaaaagg cuguaggcaa ucccccugag aaaauugcca ucgaguucac ucgagaagca     2340

gcugccaacc cacaacggac acagagccgu cucagaaccc uggagaaacu uuaugaaucc     2400

gcggaggagg ucguggaugc cagguugacu gcugaacugg cagaguuuaa agaaaauaag     2460

cacguuuuau cugacaagua cuaucucuac uucacgcagc ugggacgcga cguguauaca     2520

ggcgauacca uuagccuuga ccggcugaac gauuacgacg ucgaucauau cuugccgcag     2580

ucauuuauaa aagacgauag ucucgacaac agagugcuga cuauuagggc cguaaauaac     2640

gggaaguccg auaaugugcc cgcuaaaaug uucggaaaga aaauggguag cuuuuggcga     2700

uaucuacugg acaacggcau gaucucuaag cgcaaguaca auaaccucau uaccgauccu     2760

gacaauaucu caaaauacgc ccaaaagggg uucauaaacc ggcagcuggu ugagacaagu     2820

caggucauca aacuuaccgc aaauauuuug aacggaaucu augacaagga uacugaaauu     2880

aucgaggugc cagcgaaaau gaauagccaa augagaaaga uguuugaccu ggugaagguc     2940

agggagguua acgauuacca ccaugccuuc gacgcuuauu uaacaauauu cauuggcaau     3000

uaccucuaua aaugcuaccc caagcugcgu ccuuauuuug uguacgguaa auucaagaaa     3060

uuuuccgaua acgaagacau cgaggugggg gauggccgcu ucaauuuucu uaaccggauc     3120

gaaagacuga agaacguaac ggacccagag accggagaaa uucuaugguc uaaugucgcc     3180

cccaacgaga cuaucaagag gauuaaaaag guguacaauu auaaauucau gauaguuucg     3240

cgagagauca caacccggcg cgcagaacuc uuuaaccaga caguguaccc gaagaauuau     3300

agaggcaaac ugauuccuau caaggaggau aggccaacug acuuguacgg gggauauagc     3360

gguaacaccg augcuuaccu ggccauuguc gcauuagaag acaagaaaaa gggcaaauau     3420

uucaaggugg uugggauccc cacgcgggcc gcugcgaaac ucgagaagcu gaaacagcag     3480

gauucccaac aguaccuuca ggccgugcac gaaguaauag caccucaguu uaccaaguca     3540

acaaagaaag gaaucaagaa aacugaguuc cgaauugucc uggacaaggu gcauuacaga     3600

caauuggugc aggacggccc cgucaaaaug augcucggua guucuaccua uaaguacaau     3660

gcuaagcagc ugguucuaag cgagaaagcc cuucagguga ucaaggauga ccgcaaauuu     3720

gaugaaacac aaaaggacga uaaccuuauu gccguauaug acgagauccu cuccauagug     3780

aaucagucau ucgaucugua cgacauuaac ggguucagga aaaaguugaa ugauaaccgu     3840

aaucaguuua ucgaccugcc agcugaaacu aaguaugagg gccggaaagu cguggcacac     3900

ggaaagagag agaugaucuu agaaauucuc aaaggccugc acgccaacgc gagcuucggg     3960

aaucuuaagc cuauaggauu ugcuaccccc uucggccaga ugcaaucuaa aaacgguauc     4020

gagcuguccg aaaaugccuu uuugauucau cagaguccaa gcggccucuu cgagcacaag     4080

aucaagcugu cugaucuaug a                                               4101


<210>  3093
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3093
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uugguagcaa cuccauaggc       60

uucgccgcug ucgaugaaaa uucucaccuc aucagacuua aggggaaaac aguuauugga      120

gcaaggcugu uugaagaggg caaggccgcg gcuuaucggc gcgccucacg aacuacgcgu      180

agaagguuga gucggaaccg cuggagacug agcuuccuga gggacuuuuu cgaaucccau      240

aucaccccua cagauccaaa uuucuucaug cggcagaaau acucugagau uucgccgaag      300

gacaaggcac gcuauaaaua cgaaaagcga cuauuuaacg auagaacuga cgccgaguuc      360

uaucaacagu accccaccau guaucaccuc aggacacggc ugcuuaccga uccuucaaaa      420

gcugacgugc gugagaucua cuuugcaaua caucacaucu uaaagagccg cggucauuuc      480

uugacuccag gguacgccaa agauuuuaau acaaacaagg uagcucucaa ugaaauuuuc      540

cccgcccugc aggacgcgua ugcacaggug uacccugauc uggacaucac cuuugaugag      600

aacaagguca augaauuuaa aacggugcuc uuaaacgaga aggccacucc aagugacacc      660

caaagagcuc ugguuaaucu uuugcuggcc aaagagggag acagggauau ucuaaagcag      720

cagaaacagg ugcucacaga guucgcaaag gcugucgugg gccugaaaac uaaacuuaac      780

guagcccugg gcaccgaggu ugacuccucu gaagcgacag ccuggaauuu uagcuugggg      840

caacucgaug acaagugggc aggaaucgag uccgcuauga ccgaugaagg uacggagaua      900

cuggaccaga uucgggauuu auaucgagcc cgccugcuca acggcaucgu gcccgcuggg      960

aaaacucuuu cacaggccaa ggucgacgau uacgcacagc accacgagga ccuggaauug     1020

uucaaagauu aucuaaagca acuggaggac gacggaacag ccaaagcuau uagacagcuc     1080

uacgauaggu auaucgacgg cgaugacgcg aagccuuuug ugcgggaaga uuucgugaag     1140

gcacugacca aagaggucac ugcccaucca aaugcuaaga guccggaacu ucuggagcgc     1200

uuggcccagc ccgacuuuau gcucaaacaa agaaacaagg caaacggugc caucccuguu     1260

cagaugcagc agagggagcu ggaucaaauu auaaagaauc aggcugugua cuacgacugg     1320

uuagccgcac caaaccccgu agaaaagcac cgucagagca ugacauauca gcuggaugag     1380

cuacucaauu uccggauccc uuacuaugug gggccacugg ucaccgcgaa ggaacaaaaa     1440

gcugcccagg gaggcguguu ugcauggaug guucgaaagg accccgaugg caacauuacg     1500

ccguacaauu ucgacgagaa aguggauaga gaagccucug cuaacaccuu uauccagagg     1560

augaagacaa cugacaccua ucuuauaggg gaggacgucu ugccuaaaca gucccugcuc     1620

uaccaaucau augaggugcu gaaugaacuu aacaauguac gcguuaacga uaauaagcug     1680

agcuucgacc agaagcaaca gguguuggga gagcucuuug aacggcauaa cacagucacu     1740

auuaaacagu ucguggauaa ucuguuagcc cacggugacu acgauagaau ccccgagguc     1800

aggggccugg cagacgagaa gcgcuuucua aguucucucu ccaccuacaa ccagcugaaa     1860

gggauccuuc acgaagcuau ugaugacgcc gauaagcaga cagacaucga gaauauuaua     1920

gcguggagca ccaucuucga agauaaagcc auuuucaaga cuaaauugaa cgagaucucg     1980

uggcugaacg cuggaaugau caauucucuc gcaaacauuc gauaucgggg cuggggucaa     2040

uuuucccaua agcuguuaaa ugaacugacg cuuucaacag ggcguaccgu guucgaggag     2100

uugcucauaa guagcagaaa ccugaugcag auccuaacug acgaaguucu gaagcagaaa     2160

auugccgagc ucaaugcuca cgaacugaag gccuccggca ucuacgcagc gauugaugac     2220

gccuauaccu cuccaagcaa caaaaaggcu cuuaggcaag uguugucagu aaucgacgau     2280

auaacaaaug ccgcaggaca ggcuccuaac uggcuguaca ucgagaccgc cgacggcgau     2340

gggguccccg gaaaacgcac uaauagucgg cagagaaaga uucaggaaau cuaugcaaac     2400

gccgcucagg aguuaauuac agguuccgug aggggcgagu uugaagacaa gaucgcgagc     2460

cgagccgauu ucacggaccg ccucguucug uacuuuaugc aagggggacg ggauauauau     2520

accggcgagc cacuuaauau ugacaaccug ucuucauacg auaucgacca uauuuugccc     2580

cagagucuca ucaaagauaa uagccuggac aacagagugc uaguccaccc ucuggugaau     2640

agggaaaagg acacuauauu cgcauccaca cguuuugcua aacagaugaa cgguaugugg     2700

cgccaguggc augccgccgg gcucaucucu ucgcggaagc ugagaaaccu ucaaaugagg     2760

ccagaugaga uugacaaaua cgcuagcggc uucguagcac gacaguuggu ggaaacccgg     2820

cagaucauua agcugacaga gcaaaucguu gccgaucagu auccggacac uaagauaauu     2880

gcggucaaag cuggauuauc cucacagcuc cgcaaggagc uggauuuucc caaaaauaga     2940

gaagugaacc acuaccacca ugccuucgac gcauuucuug ccgcuaggau cggcaccuau     3000

cugcuaaagc gguacccuaa ucucgagcca uucuuuacgu augggaaauu caagaagacc     3060

gaagugaaaa agcugaaaag uuugaagucu cugaaauccu ucaacuuuau ccgagauaug     3120

acacacgcaa aggacaaaau ugucgccaag gagacuggag agaucguuug ggauaaugcg     3180

agcgacauua acgaauuaga uagaauauac aauuucaagc gcaugcucau cacccaugag     3240

gugagguuug aaacagcuuc acuguucaaa cagacucuuu augccgcaaa gaacagcaaa     3300

aaucguggug gcucucggca acugauuccc aagaaaaagg gguacuuggu ggacaucuac     3360

ggaggcuaua cccaggagac ggguuccuac cucaguguag ucagacugac aaagaaagcc     3420

auguaugcug ugguuaaggu gagcaccagg gaugccgcaa aacuagcugu cgccaagucu     3480

auuuccgaac agaaagagaa cgagacucug aagaagauca uagacgggaa acuuucaaag     3540

acaaguaaaa agggcaaaac cacucaccag cucuuugaaa ucgugcugcc ucgcguugga     3600

caaaagaccu uguucaagaa uagcaaauac aaccaguuuc ugguaaauuc ggacacauau     3660

augcacaacu accaggaguu auggaugcca cgagaauauc agagaaugug gaaggauauu     3720

cuccugucca aucauggcga cgcgcaaauc gaggggcagc uugaucagau uuucaaauuu     3780

aucgugucuc aggucaacag cuacuucaac cuguacgaca uaaaucaauu ucggaagaaa     3840

auugccgaug caacggacaa guucgcuaag uugcccaccc acgauacuga caacgccagg     3900

ggaaaaaucg cuacaaucgg ccagcuccug auuggucuac aggcaaaugc caugcgcuca     3960

gaucugcgua accuugacau aaagaccccg uucgggcucc ugcaaaucgg aacaggcauu     4020

acuuuggauu uagacaccag uaucauuuau caguccccua cgggucuguu ugaaagagag     4080

gugccacuca cagaucugug a                                               4101


<210>  3094
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3094
augaccaagg acuacagccu gggccucgau aucgggacag gauccguggg uuucgccgcu       60

cuugacacua acuauagacu guugagggca aaaggcaaua acgucauugg gguucgguua      120

uuuacgcccg ccgagaccgc ggaggagcgc cgaggauucc guacagcuag aaggcggcug      180

ucucgcagaa gguggcggcu aggcgcccuc aaugguauau uugcaccuga acuggccaag      240

guggauccaa acuuccuuca ccgccugaaa uacgcuuggg uacauccggc agaccagcaa      300

aauaagggga aauauucagc cgcucccuug uuuaacgaug ccgcgcucga ccgacaguuc      360

cacgaugacu acccuacuau cuaucaucug agacugaagc ucaugaccga uacaagucag      420

cacgacuuaa gggaggugua ccuggcaauu caucacauca uuaaguuucg gggaaauuuc      480

cuucguggcg aaggcccaau caacgccagc gaggcuuuug auauaaauac cuucaucgac      540

cagaugacug ccuuuuauga ucaagcauuc cccgacuuua aaauugucga ccagguggau      600

cagcagauca uuuucaaggg guugacagac augacccugu ccccugcuga ucgcgccaac      660

aaagcgcuaa ugggauucaa uaacgccgca caacagccaa auaagagaau cuuuaaagcu      720

auacucucug gucugguugg caaccaggug gaccuuaugg ccauuuucca gcuggauggg      780

auccccaagg aggaccaaaa ggaauuuaaa uucaaguuug ucgauaauga cauugaggaa      840

aaauuggcug cccucggaga uaacccugca cugucggacg agcagaacga guucuuaacg      900

acucuguacg ccgcuuuuuc aggccucacc cuuaaggguc uguuggggga ugcgacaagc      960

aucagugcag ccaugguggc uagguacgcc ucccaccgga cugacuggac cuauaucaaa     1020

gaccauguac uaacaccaca gaauaaccga gaacugcgcc ucgcauacgc cgaucugaug     1080

accgacaaug aggauguuag acuuaaggcu ggaaagaagu ucgccaaggc aauugaagcg     1140

gcugacauac cggccgcaaa gaagucugag uuuagccuga aaaucgauaa caaugaguuc     1200

uugcccaggc aacggacgaa ggccaacggc acuauuccuc accagcucca ucugaaugaa     1260

uuacagcaga ucauagagaa ccaaggccag uauuacccau uucugaaagc uacauucgaa     1320

caggacgggc acacccuaaa uaagauugag ggacucguga aguuucgcgu ccccuauuac     1380

guggguccuc uggugccaaa acacaagguc gccggcaaca augcaaacca uuggaugguu     1440

cccaaagaac cgggggcuau cacuccuugg aauuauacag agaaggugga uagagacaaa     1500

uccggaaacg aguucaucag gcguaugacc ggcacggaua ccuaccuuuu gggugaaccc     1560

acacugccaa agaacucacu cguauaccag gaguucaaug ugcugcaaga acuuaacaau     1620

cugcggauug agaagcgaag auuggacauc gccgucaaac aggauauuuu ugaacacgug     1680

uucaagacua acaaaaccgu uacagugaag aggauagagg aauaucucac uaccgaguuu     1740

gggaguccug ucacacugag cggcuucgcg aaugugaacg agcgcaaauu aaauucuucc     1800

cugucaaccu accauuauuu uguaggaauc uucggcgccg aauuuguuga cgaucccaag     1860

aaccuaacuc uccuggagca gauuguggaa cuucagacgg ucuucgagga ccggaaggcu     1920

uugcaaagac agcugaaacu caggccagag cugagcgaaa aacaggugaa acaguuagca     1980

cgcacacacu acaccgggug gggacgacug agucaaaaac uuuugacuuc uaaaaucguc     2040

cauguggccc ucgaggguga acgggagccc ggccaguauu ccauccugga aacccuauac     2100

gggacacaga ugaauuuuau ggagauuguu aacgacauga aggaucguua uggagugaaa     2160

agcuggcugg accaagaaaa uucgggcucu gaaaacaauc ucucccugaa gcagagaaua     2220

cuugauaugc cugguccaaa gaacaucaaa agggggauua aucaggcuuu ccacaucauu     2280

gacgagauca cccgcgccgu aggcaacgca ccgucacgga uauacaucga auuugcgaga     2340

gagagucagc aaagcaauuu gacuaacgcc agggucaacc gacugaagca guuauacucc     2400

aaucaggcuc ucaaaaagga guucgccaaa cuggcaucug aaauuaagga ucuuacaaag     2460

gagcagcuga aagacgaucg cuuguaucuc uacuaucugc aacagggaaa ggacauguac     2520

acggaaaccg auaucgacau uacuaaaauc agcucaaacu aucagauaga ucacauuauc     2580

ccccaggcuu acacaaagaa uaacagucua gacaaucggg ugcugguuaa cuccgagaug     2640

aaugccagaa aaagcgauuc uccuaccuuu cucccaucac ugauucaaga aaggaaggca     2700

ugguggcguc agcuucgcga cacaggcuug augaguacua ucaaguauaa caaucugacc     2760

cggacgagcg acgauuucuc cgccgaccag aaagagagau ucauagcuag gucuuuagug     2820

gaaacccgac aagucaucaa gaacguggcg acacucauua aagaguacua cgaugacucg     2880

cagguagagg cccugcccag cgaaaucacu gcagagaugc ggcgcuaucu uaaugugaga     2940

aagaacaggg auauuaauga cuaccaucac gcucaugaug cccugcuauu ugccaccgcu     3000

ggggaguucu cccggaaagu uggaaucaug gaaaaggguc gagucucaga cggcgcaggg     3060

aacauauaua auagauacac aaaggauauu auccagcgcg ccagggcgaa agcuggacag     3120

gacaagaaag ugcguccuuu uacuuucauc gugggcagua uggccaaccc agauaccaag     3180

uuucaaacga accagacaac cggugagauu gucuggacuc ccgaagacgu ugcauaucuc     3240

cggaaaguga ucgacuacaa gaaaaugcug gugacaaaga ugaccuugga ucagacuggg     3300

acccuguaua augagacaag auacggcucu ggagacaagc acguagccgu cgcuuuaagc     3360

aaagauaagg cagccgaccu cuacggcggg uucggauccc ugaaaccggc guaugcugcc     3420

cuuguguacu caaagaaaaa guuuaggcug guuaacgugu ugcgccucug gcugccugaa     3480

auuaagaaaa augcaagcgu ccuagagcga aaggugcagu cuguucuggg cgaugacaac     3540

gccaaaguga uaagagcuca cguaccaugc ggucaacuuc ucaucaagga uggggcccug     3600

gucacggugu ccagugcaac cgaauugcau aauuuccggc agcugugguu acccaaagcu     3660

gacuaucagg auauuagcau ccuccuggga gccccugaca aggugacugc ggagaacagg     3720

cuucaggccg agucucacuu ugaugacguc gaucaacgcu ucgaaucccu guugaaggac     3780

guuauugacc aggcacguca guacuauccc cucaaaacac aucagaccuc acuggagaag     3840

cuaaucggca aaauagguaa guuugaugaa cugaaauucg cucaaaagca acagguguuc     3900

aguagacuuc ucagcgcccu gcacgcuaau gcaacaaacu cggacuugaa gcagaucggc     3960

uuauccacug ccugggggcg gcugauuuac ccaaaaggag uaucucucac cgaugaggac     4020

aagcugauca auagcucauu uacgggccuu uucaacgaug ugaugaguau uaaagaauuu     4080

caugacuucu ccaucaauug a                                               4101


<210>  3095
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3095
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugca uuacagaccu cgauuacaau guccuuagau ucaacaaaaa ggacaugugg      120

gguucuaggc uguuugagga agccaaaacu gcugcagagc ggcgcguuca acgaaauuca      180

cguagaaggu ugaagcggcg caaguggaga uuaaaccugc uagaagagau auucaguaau      240

gaaauccuca aaauugauag caacuuuuuc aggcggcuga aggaguccuc ucuuuggcug      300

gaggacaaau cgucaaagga aaaauuuacg uuguucaaug augacaacua uaaggauuac      360

gacuuuuaua agcaguaccc caccaucuuc caccuccgca augagcugau uaaaaacccu      420

gaaaagaaag auauccgacu gguguaucuc gccauacaua gcaucuuuaa gaguagaggc      480

cacuucuuau uugaggggca gaaucugaaa gagauuaaga acuucgaaac acuuuacaau      540

aacuugaucg cguuccugga ggacaaugga auuaacaaaa ucauagauaa gaacaauauu      600

gaaaagcuag agaaaaucgu augugacucc aagaaaggcc ucaaggacaa agaaaaggag      660

uuuaaggaga uuuucaacuc ugauaaacag cugguggcua ucuuuaaacu uagcgucggu      720

uccucaguga gucugaauga cuuguucgau acugacgaau acaaaaaggg ggagguugaa      780

aaagagaaga ucagcuuuag ggaacaaauu uaugaggaug acaagccaau auacuauucu      840

auccucggag agaaaauuga acuguuagau aucgccaagu ccuucuacga cuuuauggug      900

cugaacaaua uacucgcaga uucacaguau auuagcgagg ccaaagucaa acuuuacgag      960

gagcauaaaa aggaccugaa gaacuugaaa uauaucaucc ggaaguacaa uaaaggcaac     1020

uacgauaagc uauucaaaga caagaaugaa aacaauuaua gugcuuacau uggccugaac     1080

aaagagaagg ggaagaaaga ggugguagaa aagucucguc ucaaaaucga cgaucugauu     1140

aagguuauaa aaggauaucu uccgaagccc gagcgcaucg aagagaagga caaaaccgug     1200

uuuaaugaga uucuguccaa gaucgaauug aaaacaaucc ucccuaagca gagaauuagc     1260

gauaacggua cccugccaua ccagauacac gaggucgaau uagagaaaau ccuggaaaau     1320

caaucgaagu auuacgacuu ccuaaacuau gaggagaacg gcgugucuac uaaggauaaa     1380

cuccugaaga cauuuaaauu caggauuccc uacuacgugg ggccucuuaa uuccuaucau     1440

aaggacaaag gaggcaacuc auggaucguc cggaaggaag aggguaagau uuugccaugg     1500

aauuuugaac agaaaguuga uaucgagaag agugcagaag aguucauaaa acgaaugacc     1560

aacaagugca cguaccugaa uggggaggac gugaucccca aagauacuuu ccucuauagc     1620

gaauacguaa uucugaacga gcuuaauaag gugcagguca acgacgaauu ucugaaugag     1680

gaaaacaaac gcaagaucau ugaugaguug uucaaggaga auaaaaaggu guccgaaaag     1740

aaguuuaaag aguaucuccu gguuaagcag aucguggacg gaaccauaga auuaaagggc     1800

gucaaagauu cuuucaacag caauuacauu ucauauauca gauuuaagga cauuuucggc     1860

gagaaacuga accuagacau cuacaaggag auaagugaaa aauccauccu cuggaagugu     1920

cuguacgggg augacaagaa aauuuuugag aagaaaauca agaaugaaua uggagauauu     1980

cuuacaaaag acgagaucaa gaagauaaac acuuucaaau uuaacaauug ggguagguug     2040

agcgaaaagc ugcucaccgg cauugaguuc aucaaccugg agacagggga augcuacucu     2100

ucagugaugg augccuuacg gcgcaccaau uauaaccuga uggagcuuuu gaguagcaaa     2160

uuuacgcucc aagaauccau caauaacgag aauaaggaaa ugaacgaggc uucuuacaga     2220

gaccugauug aggaaucgua uguaagcccu ucccuaaaaa gggccaucuu ccagacucug     2280

aagauuuacg aggaaauacg uaagaucaca ggacggguuc caaagaaggu guuuauugag     2340

auggcgcgag gcggugauga aucaaugaaa aauaagaaaa ucccggcaag acaggagcaa     2400

cucaagaaac uguaugacag uugugggaac gauauugcca auuucucuau cgacauaaag     2460

gagaugaaga acagccuuau cuccuacgau aauaacucau ugaggcagaa aaagcuguac     2520

uuauauuacc uccaguucgg caaaugcaug uauaccggac gcgaaauuga ccuggaucgg     2580

cuucugcaga auaacgacac uuacgacauc gaucacauuu aucccagaag caaggucauc     2640

aaagacgauu cuuuugacaa cuuggugcuc guccugaaga augagaacgc ugaaaagucc     2700

aaugaguacc cugugaaaaa ggagauacaa gaaaagauga agaguuucug gagguuucua     2760

aaagagaaga acuucauuag cgaugaaaag uauaaacgcc ugacaggcaa ggacgauuuu     2820

gagcuccgag gguucauggc ccggcagcug guuaaugugc gucagaccac gaaagaagua     2880

ggaaagaucc uucagcaaau cgagccagag auuaaaauag ucuacucuaa ggcagaaauc     2940

gcuuccucau uuagagagau guucgacuuu auuaagguga gggaauugaa cgauacccac     3000

caugccaaag acgcguaccu gaauaucguu gccgguaacg uguauaauac aaaguucacu     3060

gagaaacccu accgcuauuu acaggaaauu aaggagaacu acgaugucaa aaagaucuau     3120

aauuacgaca ucaaaaacgc augggacaag gagaauaguc ucgaaauagu gaagaaaaac     3180

auggagaaga auaccguaaa cauuacacgg uuuaucaaag aagagaaggg ccagcuguuc     3240

gaucuuaauc cuauuaaaaa gggggaaacu agcaacgaga ucauaucgau uaagccaaaa     3300

guguauaacg gaaaggacga uaaacugaau gagaaguacg gcuacuauaa aucccuaaac     3360

cccgcuuacu uccucuaugu ugaacacaag gagaagaaua aaagaaucaa gucuuuugaa     3420

agggucaacc ugguggacgu gaauaacauc aaagaugaaa agagcuuggu caaauaccug     3480

auugagaaua agaaguuagu ugaaccgcga gugaucaaaa agguguauaa acgccaggua     3540

auacucauua accauuaccc uuauucaauc gucacccugg acaguaauaa acuuauggau     3600

uucgagaacc ugaaacccuu guuucucgaa aauaaguacg agaaaauucu gaagaacgug     3660

aucaaguucc uagaagacaa ucaagguaaa uccgaggaga acuacaaguu uauauaucug     3720

aaaaagaaag aucgguacga aaagaaugag acacuugaau cuguuaagga cagauauaac     3780

cucgaguuca acgaaaugua cgauaaauuu cuggagaagu uggacagcaa agauuauaag     3840

aauuacauga acaauaaaaa guaucaggag cuguuagacg ugaaggaaaa auucauuaag     3900

cucaaccugu uugacaaagc cuucacccuu aaguccuuuc uggauuuguu caauaggaaa     3960

acuauggcug acuuuucaaa ggucgggcuc acgaaguacc ugggcaaaau ccagaagauc     4020

agcaguaacg ugcuaucuaa aaaugagcug uaccuucucg aagaguccgu uacaggacug     4080

uucgugaaga aaauuaaguu guga                                            4104


<210>  3096
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3096
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagaccu cgauuacaau guucuuagau ucaacaaaaa ggacaugugg      120

gguucuaggc uguuugauga ggccaaaacu gcugcagaac ggcgcgugca acgaaauuca      180

cguagaaggu ugaagcggcg caaguggaga uuaaaccugc uagaggaaau uuucagugac      240

gagauaauga aaaucgauag caacuuuuuc aggcggcuca aggaauccuc ucuguggcuu      300

gaggacaaaa auucgaagga gaaauuuacg cuguucaaug augacaacua uaaggauuac      360

gacuuuuaua agcaguaccc caccauuuuc cacuugcgcg augaacucau caaaaauccu      420

gagaagaaag acauucgacu gaucuaucug gcccuccauu caauauuuaa gagcagaggc      480

cacuucuuau uugaagggca gaaccugaaa gagaucaaga auuucgagac acuuuacaac      540

aauuugauua guuuccugga agacaacgga aucaauaaau ccauugauaa ggacaacauc      600

aagaaacuag agaagauaau uugugauucu ggcaaagguc ucaaggacaa agaaaaggag      660

uuuaaggaaa ucuucaacag cgauaaacag cugguagcga uuuuuaaacu uuccgugggg      720

ucaaguguca gccugaauga cuuguucgau acugacgagu acaaaaagga ggaaguggag      780

aaagaaaaga ucucuuuuag ggagcaaauc uaugaagaug acaagccaau uuacuauucc      840

auacucggag agaaaaucga gcuguuagau auugcuaagu cauucuacga cuuuaugguu      900

cugaacaaua uccucagcga caguaacuau auaucugaag ccaaagugaa acuuuacgag      960

gaacauaaaa aggaucugaa gaauuugaaa uauauuaucc ggaaguacaa caaagagaau     1020

uacgacaagc uauucaaaga uaagaacgaa aauaacuauc cggcauacau cggccugaau     1080

aaagagaagg gcaagaaaga ggucguggaa aaguccgggc ucaaaauuga cgaucugauc     1140

aagguaauua aaggauaucu ucccaagccu gagcguauag aagagaagga caaaaccauc     1200

uuuaacgaga uucugaauaa gaucgaauug aaaacaaucc ucccaaagca gcgcauuagc     1260

gauaacggua cccugcccua ccagauacac gagguugaau uagagaaaau ccuggaaaau     1320

cagucgaagu auuacgacuu ccuaaacuau gaggagaacg gcgugucuac uaaggauaaa     1380

cuccugaaga cauuuaaauu caggauuccu uacuacgucg ggccacuuaa uuccuaucau     1440

aaggacaaag gaggcaacuc auggaucgug agaaaggaag aggguaagau uuugcccugg     1500

aauuuugaac aaaaagugga uaucgagaag agugccgaag aguucauaaa acggaugacc     1560

aacaagugca cguaccugaa uggggaggac gucaucccua aagauagcuu ccucuauucc     1620

gaauacauua uccugaacga gcuuaauaag guucagguga acgacgaauu ucugaaugag     1680

gaaaacaaac gaaagauuau cgacgaguug uucaaggaga auaaaaaggu aucugaaaag     1740

aaguuuaaag aguaucuccu ggugaaccag auagcuaauc gcacugucga auuaaaggga     1800

auuaaggaua gcuucaacuc aaauuacgug aguuauauca aauuuaagga cauuuucggc     1860

gagaaacuga accuagauau cuacaaggag auauccgaaa agagcauccu cuggaagugu     1920

cuguacggcg acgauaagaa aauuuuugag aagaaaauca agaacgaaua uggggacauu     1980

cuuaauaaag augagaucaa gaagauaaac ucuuucaaau uuaauaccug gggaagauug     2040

ucagaaaagc ugcucacagg uauugaguuc aucaaccugg agacuggcga augcuacagu     2100

agcguuaugg aggcauuaag gcggaccaau uauaaccuga uggaacuuuu guccucuaaa     2160

uuuacacucc aggagucgau cgacaauggg aacaaggaaa ugaaugaggu gagcuaccgc     2220

gaucugauug aggaauccua ugucucacca agucuaaaaa gagccauccu gcaaacccuc     2280

aagauuuacg aggaaauaaa gaaaaucacg ggaagggugc cgaagaaagu auucauugag     2340

auggcucgug gcggugacga aacuaugaag aacaaaaaga uccccgcccg gcaggagcag     2400

cugaaaaagc uauaugauuc uugugggaau gacauugcga acuuuagcau cgauauaaag     2460

aaaaugaagu ccucauugag cucuuacgac aauaacuccc ugcgacagaa aaaguuauac     2520

cucuauuacc ugcaauucgg caaaugcaug uauacaggaa gagagaucga ccuugauagg     2580

cuguugcaga auaacgacac cuacgauauu gaccacaucu auccucgcag uaagguuauu     2640

aaggaugaca gcuucgauaa ccucgugcug guccuuaaaa augaaaacgc agagaagucu     2700

aaugaauacc cagugaaaaa ggagauccag gagaaaauga aguccuuuug gcgguuccug     2760

aaggagaaaa acuuuauauc agacgagaag uauaaaagac ucacuggcaa ggaugacuuc     2820

gaacugaggg gguuuauggc ccgccaacuu gucaaugugc gacagacaac caaagagguu     2880

ggaaagauuu ugcagcagau cgaacccgag aucaagauug uguacaguaa agcugagaua     2940

gccagcucgu uccgggaaau guuugauuuc aucaagguac gugagcugaa cgacacgcac     3000

caugcaaaag acgcuuacuu aaauauuguc gccgguaacg uguauaauac caaguuuaca     3060

gaaaaaccuu acagauaucu ccaagagauc aaggaaaacu acgauguuaa aaagauuuau     3120

aauuacgaca ucaagaacgc gugggauaaa gaaaauuccc uggagauagu gaagaaaaac     3180

augaagaaaa auacugucaa caucaccagg uucauuaagg aagagaaggg ccagcuuuuu     3240

gaccugaauc caaucaaaaa gggggaaaca ucuaacgaga uuaucagcau aaaacccaag     3300

cuauauaacg gaaaagaaga gaagcucaau gagaaguacg gcuacuauaa aucacugaac     3360

ccggccuacu ucuuguaugu ggaacacaag gagaaaaaua agcgcauuaa aaguuucgaa     3420

cggguaaacc ugguggaugu uaauaacauc aaggacgaga aguccuuagu caaauaccuc     3480

aucgagaaua agaaacuggu ggaaccuaga cuuauuaaga aaguguauaa gaggcagguc     3540

auccugauaa acgauuaccc cuauucuauu guugcauugg acagcaauaa acucauggau     3600

uuugagaacc ugaagccacu auuccuggaa aauaaguacg agaaaauccu uaagaacgug     3660

auuaaauuuc ucgaagacaa ucaggguaag uccgaggaga acuacaaauu caucuaucug     3720

aagaagaaag aucgauacga aaagaaugag acuuuggaau cagugaaaga ccgcuauaac     3780

cuggaguuua acgaaaugua cgauaaguuc uuagagaaac ucgacagcaa ggacuauaag     3840

aauuacauaa acaauaaaaa guauaaaaac cugcuugaug uaaaggagaa auuuauuaag     3900

cugaauuugu ucgacaaggc uuuuacccuc aaaaguuucc uggaucuauu uaaccggaag     3960

acaauggccg acuucucuaa agucgggcug accaaguacc uuggcaaaau ccaaaagauc     4020

uccagcaaug ugcucucgaa gaacgaacug uacuuguuag aggaaucagu uacuggacug     4080

uuugugaaaa agauuaaacu cuga                                            4104


<210>  3097
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3097
augaagaaac agcaauucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagacuu caauuacaac guucucagau uuaauaagaa agauaugugg      120

gguucuaggc uuuucgacga ggccaagacu gcugcagaac ggcgcgugca gcgaaacuca      180

cguagaaggc ugaaacggcg caaguggaga uugaauuuac uggaggaaau uuuuagugau      240

gagauacuaa agaucgacag caacuucuuu aggcggcuca aagaauccuc ucuguggcuu      300

gaggauaagu cgucaaaaga gaaguucacg cuguuuaaug acgauaacua uaaagacuac      360

gauuucuaua agcaguaccc caccauuuuu cacuugcgca augaacucau caagaacccu      420

gagaaaaagg acauucgacu gguauaucug gcccuccaca gcaucuucaa aaguagaggc      480

cacuuuuuau ucgaagggca gaaucugaag gauauaaaga acuucgagac acuuuacaau      540

aacuugaugg cguuucugga ggacaaugac aucuacaaga acauugauuc cucuuauauc      600

ggaaaccuag aaaauauuau cugugacagc aaaaagggcc ucaaggauaa agagaaggag      660

uucaaagaga uauuuaacuc cgacaagcaa cugguggguu ucuuuaaacu uucagucggg      720

aguagcgugu cucugaauga uuuguucgac acugaugaau acaagaaggg agagguugag      780

aaagaaaaga uuuccuuuag ggagcagauc uaugaagacg auaaaccaau uuacuauuca      840

auccucggcg agaagaucga guuccuggac auugcuaaau cauuuuacga uuucauggug      900

uuaaacaaua uacuggccga cagucaguau aucucugaag caaaggucaa gcucuacgac      960

gagcauaaac gggaucuuaa gaaccugaaa uacauuaucc guaaguauaa uaaagaaaac     1020

uacgacaagu uguuuaagga uaaaaaugag uccaacuaua gcgccuacau aggccuaaau     1080

aaggaaaaag ggaagaaaga ggugauugaa aagucgcgcc ugaaaaucga cgauuucgcu     1140

aagaucauua agggauaucu cccgaaagca gagaagaucg acgagaaaga uagaucuauu     1200

uuuaacgaaa uacuggacaa gaucgagcuu aaaaccauuc ugcccaagca gaggaucucc     1260

gauaauggua cauugccuua ccaaauccac gaagccgagc ucgagaagau ucuggaaaac     1320

caggcuaaau auuacgacuu cuuaaaucau gaggaaaacg gcauaucaac caaggauaaa     1380

cugcuaauga cuuucaaguu ucggauccca uacuauguag ggccccucaa caguuaccac     1440

aaaaauaagg gaggcaacag cuggauuguu cgaaaggagg aagguaaaau ccugccuugg     1500

aauuucgagc agaaggugga cauugagaaa uccgccgaag aguuuaucaa gcgcaugaca     1560

aacaaaugca ccuaucuuaa uggggaagau gucauaccaa aggacucuuu cuuguacagc     1620

gaguauauca uucugaacga acucaauaag guccagguga acgacgaguu ucugaauaaa     1680

gagaucaaga aaaagauuau cgaagaucuu uucaaaaaga gcaaaaagau aagugagaag     1740

aacuuuagag aauaccuguu ggugaaucaa auuacgaaca aaacuguuga gcucaaggga     1800

gugaaagacg cguucaauuc caacuaugua agcuacauca aguuuaaaga uauuuucggc     1860

gacaagcuga auuuagauau cuacaaggaa auaucugaga aaucaauccu guggaagugu     1920

cuauauggcg acgauaaaaa gauuuuugag aaaaagauca agagugugua cggggacauu     1980

cucaccaaag augaaaucaa gaaaauaaac agcuucaagu uuaacacaug gggaaggcug     2040

uccgagaaac uuuugacuga aauugaguuc aucgaccugg agaccgguga augcuauucu     2100

ucggucaugg augcacuccg gcgcacaaau uacaaccuga uggaguuacu gagcuccaag     2160

uucacccuuc aggaaaauau cgacaacgag aauaaggaag ugucagaguu uaguuauaga     2220

gauuugguug aggaaucuua cgugagcccc ucccucaaaa gggccauucu gcagacgcua     2280

aagaucuaug aggaaauucg uaaaauaacu ggccggaucc cuaagaaagu cuucauugag     2340

auggcucgag ggggagacga aacaaugaag aacaagaaaa ucccagccag acaagagcag     2400

cugaagaaac ucuacgacuc auguggcaag gauauuagca auuuuucuau cgacauaaaa     2460

gagaugaaga acucccugaa uaguuacgau aacaauagcc uuaggcagaa aaaguuguau     2520

cuguacuauu uacaguucgg uaagugcaug uacaccggga aagaaaucga ccucaaccgc     2580

cugcuucaaa auaacgauac uuaugacauu gaucacaucu acccgcgguc uaaggugauu     2640

aaagacgauu ccuuugacaa ucugguauug guucucaaga acgagaacgc agaaaaauca     2700

aaugaguauc cccugaagaa ggaaauccag gagaaaauga agaguuucug gaaauuucua     2760

aaggagaaaa acuucauaag cgaugaaaag uacaagagac ugacaggcaa agacgaguuu     2820

gaacucaggg gauucauggc ucgccagcug gugaaugucc gacagaccac gaaggaggcc     2880

ggcaaaauuc uucaacagau cgagccugaa aucaagauug uguacucgaa agcggagaua     2940

gccuccucuu uucgggaaau guucgacuuc aucaaggucc gugaguugaa cgauacccau     3000

cacgcaaagg acgcuuaucu gaauauugug gccgggaacg uuuacaauac aaaauuuacu     3060

gaaaagccau auagauacuu acaggagauc aaagagaacu augaugugaa gaaaauuuac     3120

aauuaugaca ucaagaacgc uugggauaaa gaaaagagcc ucgagauagu aaagaaaaau     3180

auggaaaaga acaccgucaa uaucacaagg uucauuaaag aggaaaaggg acagcuguuu     3240

gaccuuaacc ccauccgcaa aggugagacu ucaaaugaga uuaucgccau aaagccuaag     3300

uucuacgaag gcaguaccga gaaacugaac gaaaaguacg gguauuacaa aucccuaaau     3360

ccagcauauu uuauuuacgu ggagcauaag gagaaaaaca agaucauccg gucuuucgaa     3420

agaguuaacc ucguggaugu caauaagauu aaagacgaaa agagccugau caaauauuug     3480

auagaaaaca agggacugau ugagcccaaa gugaucaaga agguauacaa aaggcaagug     3540

auuuuaauca auaacuuucc guauuccaua guugcccucg auucaaauaa gcugauggac     3600

uucgaaaacc uuaaaccucu guuuuuggag cgaaaguacg agaaaauucu caagaaugcu     3660

aucaaauucc uggaagauaa ccagggcaag acagaggaau acuauaaguu ucuauaccug     3720

aaaaagaaag accgcaauga gaagaacgaa accaucgaua gcgucaaaga gcgguauaau     3780

auugaguuca acgaaaugua cgacaaguuu cuugagaagc ucgacaguaa agauuauaag     3840

aauuacauca acaauaaaaa guauucugaa cuggugaacg ugaaagagaa guucauuaag     3900

uugaaccugu ucgacaaagc guuuacuuua aaguccuucc ucgaucuguu uaauagaaaa     3960

acgauggcag acuucagcaa ggucggucuu acaaaauacc uggggaagau acagaagauc     4020

ucgucaaacg uuuugucuaa aaaugaacuc caucugcuag aggagagugu gaccggccug     4080

uuugugaaga aaauuaaacu uuga                                            4104


<210>  3098
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3098
augaaggacu acuaucuggg ccucgauaug gggagcuccu cugugggaug ggcacuuuca       60

gacgagagau acaaccuguu gagggcuaaa gguaaggauu uauggggcgu ccggcuguuc      120

gacgaagcaa aaaccgccgc ggagcgccga acauuucgua gucagagaag gcggcuagaa      180

cgcagcaagu ggagacucuc ccugcuuacu ucucuguucg aggaagagau cucgaaaauu      240

gaugacaagu uuuucgauag guugaaggac ucaaaauuuu ucauagauga caagagcgau      300

caacagccca auacgcucuu uaacgacguu aauuucaccg auaaagagua ucacaagcgg      360

uacccuagua ucuaucaucu gcgcaacgcu cuguuuaauc agaacgacaa auucgacauu      420

cgacucaucu uuuuagcccu gucccacauu gugaagaaga gagggcauuu ccuuuuugau      480

ggagacaaag caggcuucgg ugaagauauc gacguaauau uggaugagcu gaaggccuac      540

cuacaggacg cuuuccacac aaaacucgaa uuuacuucug aguucaagag caucaugcug      600

gauaaaaauc uuaggaagaa acaaaaggag gaggaacuga agaacuccau uaaaaucuca      660

agugacacca uuaauagcaa ggaugggaaa ucuaucuuga uaaagcucuu ucugggauau      720

gaagcaaacu uaaaaccauu cuuucuggag uacaaggugg aaaaggacaa ugaguacaaa      780

auuaacuucu ccaaggagga uuaugaagag aaacuccuug acaugacauc aaaguuugaa      840

gagaccacuu acuucaucga aauugccaaa aagauccaug acuauuuucu guuggaggag      900

aucaugcuag gcuucaagaa uauuagcgcu aguaaagucu cuauauacca gaagcacaaa      960

aacgaucuga agacacucaa aaauaucgug aagaaccaga uuaacaauuc cuauaacgcc     1020

aucuuuaagg acauaggccu ggauaauuac ccggcguaug uuagcugcug gaacucgaaa     1080

aagcacaaag aaaagcagaa aggguguacc aaggacaaau ucuacgcauu ucuuaagacg     1140

aagcugaaaa auauuaacaa ugccaaggag aucuuggauu cuaucgacaa cgaugacuuc     1200

cucccccggc ugguggauaa aaauuccauu aucccucauc aaauucacuu agcugaacug     1260

auaaagauca uugacaacca gucacaguac uauccauucc uaaaagagau caaggauaag     1320

aucauuagua uauuuaaauu ccguaucccc uuuuacgucg gaccucucaa uaacuuccau     1380

aaggacaaag guggcaaugc cuggauugaa aagcugaaag augagcgcau cuuuccaugg     1440

aacuucaaug acgugguaga ccuuaacaag agcgcagcua acuuuauuga gaagaugauc     1500

aaugaaugcu ccuauuugaa aggggagaag guucuggcca aaaacucucu ccuguacacu     1560

aaguauagcg ugcuuaaucu gauaaacaaa uugaagauca auggaaccgc ggucucagug     1620

gaaacaaagc agaaaauuua caacgagcuc cuguuaaagc aaaagaauau caagaaaacu     1680

gccauuaaga aauuccugau caacaauagu auaauugaua agaacgacga gaucuccggc     1740

auugaugaca gccuaaccuc uaaucucgca guguuuaucg auuucgaaaa gauaucaaac     1800

agacugaggu augaccagaa agaggauauc auuaagagua ucagccuuuu ugguuacgac     1860

aaugcuaugu ugaaaaagca gauuaucaca auagcccccg aucuguccga agacgagcuc     1920

gaaaaacugu cuaaguuacg guacucgggg uucggacgac ugagcaagcg ccuuuugacc     1980

uccauuaaac acaacggcaa gucaaucauc gagaugcucu gggauacgaa ugacaaccug     2040

auggagcuac ugagugauag auauacuuuu auugaacaaa ucaacaaaca caaugacggc     2100

uucaaggagg acauuaaccu caauuuccag aacgucgaua cacuguacgu uucuccuagc     2160

gugaaaaggc agauauggca ggcuuuuaag guaaucaaug aacuuauuuc cgugaugggg     2220

cgggagccaa agaaaaucuu cgucgaaaug gcccgcgagg agggagaaaa gaaaagaacc     2280

caagcaagga aggacaaagu guuggagcug uauaagucaa uuaagaacaa ugaaaucagc     2340

acuuucuuaa caaacuucaa agauauaaag accaaacucg agggugaauc uaaucugaac     2400

uccaagaaac uuuaccugua uuacuugcag cucggcguug acaucuauac gggggagaag     2460

auuccguuug agaagcuguu cgaucagaau aucuacgaca uugaucaugu gaucccccgu     2520

agucugaaaa aggacgauag cuuugacaac cuaguccuca ccucuaaaac aaauaacgcc     2580

aagaaaacug augaauuucc uguguccuca gagauacaga guaagaugca aaccuuuugg     2640

aaaguacuga aggacaagga ccuuaugagc gaaauuaaau acacaaauuu gacucggaag     2700

acccaccugu cggaugacga guuaggagau uucaucgcuc gacagcucgu ugagacaaga     2760

caguccacca aagcgcugac ucagauccuu aagaacgugu augcaaacuc ugacauuguc     2820

uacgugaaag ccggcauagu cagcgaauuu aggcaacagu ucgauaugcu gaagauccgc     2880

gagcuaaaug acuuccauca cgcuaacgau gccuaucuca auauugugau cgguaacguu     2940

uacaauauua aguuuacgaa aaacccacgg aauuauguga aggaauacua uaaaaagggg     3000

gagcauuaca accugaaaau cgaaaagaug uuggagaagg acauagcaag aggcggaaau     3060

uucggcuggg auaaaaagca cucaaucgag acaguacaga aaaccaugaa gaaacagagu     3120

ccccugauua cuuacaggac cuuugaaaac aagggggagu ucuuuaauca acagccuucc     3180

aaggccucug cuaacuuacu cgcccugaaa agcuccgaca agcgccuucu ggauacaguc     3240

cgauauggag guuacaaagg cacccaguau gcacacaugu ucuuggugga acauuuuaau     3300

gggaagauca uuaaagagau caugggaguu ccaauauacc ucucaaagga aauuaaagac     3360

gauaaggcga agcugcuaga guauuguaac gacgugcuga aacuuaagaa ucccaaaauc     3420

cucuucagca agaucccgua caacagucug auugucuaua auggcuaccc uaucuugcug     3480

cgggguuuaa cuggggugcg uggcgcugcc caacucaaac uggaccagac aucuacgcag     3540

guagugaaga acauaguuaa acuuuacgau uggcuguccc agaaacaaaa ggaguugagc     3600

gaccucucga aaaagauuga auuuuuccag gagaacaaua aaaaggauga agucaaggca     3660

gccaugcugg aggcugacaa cauccuauuu auucugaaug auagauucaa aaacaccaag     3720

aucggaaaag acauaaugaa ugauaaccac cuuaauaacc ucuaugacac uuuuauuaau     3780

aagcuggaaa acucaaucua caaaaauaag cccggcggga agcaggaugu guugaugaaa     3840

gagaagaggg acuuauuccu gucuuucagu auguccaaaa agugcaucac acucuauaaa     3900

auuaucaaga uuuuuggaag cugugaucag gguguggacu ucucugauau agccgagaaa     3960

aucagcaagu ccggcgggau uggaggcaag aaccugggua aaaauaccuu ugaccuuguc     4020

uucaaggaaa acucaacaua cacucugguu caaaguagcg ugaccggguu guaugccaaa     4080

aagacggacc uacauaaacu guga                                            4104


<210>  3099
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3099
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagaccu cgauuacaau guucuuagau ucaacaaaaa ggacaugugg      120

gguucuaggc uguuugagga agccaaaacu gcugcagagc ggcgcgugca acgaaauuca      180

cguagaaggu ugaagcggcg caaguggaga uuaaaccugc uagaagagau uuucaguaau      240

gaaauacuca aaaucgauag caacuuuuuc aggcggcuga aggaguccuc ucuuuggcug      300

gaggacaaau cgucaaagga aaaauuuacg uuguucaaug augacaacua uaaggauuac      360

gacuuuuaua agcaguaccc caccauuuuc caccuccgca augagcugau caaaaacccu      420

gaaaagaaag auauucgacu gguauaucuc gccauccaua gcauauuuaa gaguagaggc      480

cacuucuuau uugaggggca gaaucugaaa gagaucaaga acuucgaaac acuuuacaau      540

aacuugauug cguuccugga ggacaaugga aucaacaaaa uuaucgauaa gaacaauaua      600

gaaaagcuag agaaaauugu gugugacucc aagaaaggcc ucaaggacaa agaaaaggag      660

uuuaaggaga ucuucaacuc ugauaaacag cuggucgcua uuuuuaaacu uagcgugggu      720

uccucaguua gucugaauga cuuguucgau acugacgaau acaaaaaggg ggagguggaa      780

aaagagaaga ucagcuuuag ggaacaaauc uaugaggaug acaagccaau uuacuauucu      840

auacucggag agaaaaucga acuguuagau auugccaaga ccuucuacga cuuuaugguc      900

cugaacaaua uccucgcaga uucccaguau auaucagagg ccaaagugaa acuuuacgag      960

gagcauaaaa aggaccugaa gaacuugaaa uauauuaucc ggaaguacaa uaaaggcaac     1020

uacgauaagc uauucaaaga caagaaugaa aacaauuaua gcgcuuacau cggccugaac     1080

aaagagaaga guaagaaaga gguaauugaa aagucucguc ucaaaaucga cgaucugauu     1140

aagaauauaa aaggguaucu uccgaagguu gaggaaaucg aggagaagga caaagcaauu     1200

uuuaacaaga uccugaauaa aaucgaauug aagacaauuc uccccaaaca gcgcauaucc     1260

gauaacggaa cccugccuua ccagauccac gaggccgaau uagagaagau ucuggaaaac     1320

caaagcaagu auuacgacuu ccuaaauuau gaggagaacg guaucauuac uaaagauaag     1380

cuccugauga cauuuaaauu cagaauccca uacuacgugg gcccccuuaa uucguaucau     1440

aaggacaaag ggggaaacuc uuggauaguc aggaaggaag agggcaagau cuugccuugg     1500

aauuuugaac agaaagugga uauugagaag uccgcugaag aguucaucaa acggaugacc     1560

aacaagugca cguaccugaa uggugaggac gugauuccaa aagauacuuu ccucuauuca     1620

gaauacguca uccugaacga gcuuaauaag guucagguga acgacgaauu ucugaaugag     1680

gaaaacaaac gaaagauaau ugaugaguug uucaaggaga auaaaaaggu aagugaaaag     1740

aaguuuaaag aguaucuccu ggugaagcag aucgucgacg ggaccauuga auuaaaggga     1800

gugaaagaua gcuucaacuc caauuacauc ucuuauauac gcuuuaagga caucuucggc     1860

gagaaacuga accuagacau uuacaaggag aucagcgaaa aaucaauucu cuggaagugu     1920

cuguacggcg augacaagaa aaucuuugag aagaaaauaa agaacgaaua uggggauauu     1980

cuuacaaaag acgagaucaa gaagaucaau acuuucaaau uuaacaauug gggaagauug     2040

agugaaaagc ugcucaccgg uauugaguuc aucaaccugg agacaggcga augcuacucc     2100

agcguuaugg augccuuaag gcggaccaau uauaaccuga uggagcuuuu gucuucaaaa     2160

uuuacgcucc aagaaaguau uaauaacgag aauaaggaaa ugaacgaggc gagcuaccgc     2220

gaccugauag aggaauccua ugugucuccc ucgcuaaaaa gagcaaucuu ccagacucug     2280

aagauuuacg aggaaaucag gaagauuaca gggcgugucc cuaaaaaggu guuuaucgag     2340

auggcccggg gaggcgauga aagcaugaaa aauaagaaaa uaccagcucg acaggagcaa     2400

cucaagaaac uguaugacuc cugugguaac gauaucgcca auuucucaau ugacaucaag     2460

gagaugaaga acagucuuau uucuuacgau aauaacagcu ugagacagaa aaagcuguac     2520

uuauauuacc uccaguucgg gaaaugcaug uauaccggca gggaaaucga ccuggaucgc     2580

cuucugcaga acaaugacac uuacgacaua gaucacauuu auccgcgguc caagguaauc     2640

aaagacgauu cauuugacaa cuugguucuc gugcugaaga augagaacgc agaaaagagc     2700

aaugaguacc ccgucaaaaa ggagauccaa gaaaagauga agucuuucug gagauuucua     2760

aaagagaaga acuucauuuc cgaugaaaag uauaaaaggc ugacaggaaa ggacgauuuu     2820

gagcuccgcg gcuucauggc ucgacagcug gugaaugucc ggcagaccac gaaagaagug     2880

gggaagauac uucagcaaau cgagccugag auuaaaaucg uuuacaguaa ggccgaaauu     2940

gcgagcucuu uucgugagau guucgacuuu aucaagguga gagaauugaa cgauacccac     3000

caugccaaag acgcauaccu gaauauagua gcuggaaacg ucuauaauac aaaguucacu     3060

gagaaaccau acagguauuu acaggaaauc aaggagaacu acgaugugaa aaagauuuau     3120

aauuacgaca ucaaaaacgc cugggacaag gaaaauuccc ucgaaauugu uaagaaaaac     3180

auggagaaga auaccgugaa caucacacgc uuuauaaaag aaaagaaagg ucagcuguuc     3240

gaucuuaacc ccauuaagaa gggcgagacu ucaaaugaaa ucaucaguau uaaaccuaag     3300

gucuauaacg ggaaagacga uaagcugaau gagaaauacg gauacuauaa gagccuaaac     3360

ccagcuuacu uccucuaugu ggagcacaag gagaaaaaua agcggaucaa aucguuugaa     3420

agaguaaacc ugguggacgu uaauaacaua aaggaugaaa aauccuuggu caaguaccug     3480

auugagaaua agaaauuagu ggagcccagg gugaucaaga aagucuauaa gcgacagguu     3540

auucucauca acgacuaccc guauucuaua gugacccugg auagcaauaa acuuauggac     3600

uucgaaaacc ugaagccuuu guuucucgag aauaaauacg aaaagauucu gaagaacgug     3660

aucaaauucc uagaggauaa ucaaggcaag ucagaagaga acuacaaauu uaucuaucug     3720

aagaaaaagg accgcuacga gaagaacgaa acacuugaga guguaaaaga ucgguauaau     3780

cucgaguuca acgaaaugua cgacaaguuu cuggaaaaau uggauuccaa ggacuauaaa     3840

aauuacauga acaauaagaa guaucaggag cuguuagacg ucaaagagaa guucauuaaa     3900

cucaaccugu uugauaaggc cuucacccuu aaaucuuuuc uggacuuguu caauagaaag     3960

acuauggcag auuuuagcaa ggugggucuc acgaaauacc uggggaagau ccagaaaauu     4020

uccucaaacg uucuaagcaa gaaugaacug uaccuucucg aggaaagugu gacaggccug     4080

uucgucaaaa agauaaaguu guga                                            4104


<210>  3100
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3100
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagacau gaauuacaac guucucagau ucaauaaaaa ggauaugugg      120

gguucuaggc uuuuugagga agccaaaacu gcugcagagc ggcgcgugca acgaaacuca      180

cguagaaggc ugaagcggcg caaguggaga uugaauuuac uggaagagau uuucaguaac      240

gaaauacuaa aaaucgacag caauuucuuc aggcggcuca aggaguccuc ucuguggcuu      300

gaggauaaau cgucaaagga aaaauuuacg cuguucaacg acgauaauua uaaggacuac      360

gauuuuuaua agcaguaccc caccauuuuc cacuugcgca acgagcucau caaaaauccu      420

gaaaagaaag acauucgacu gguauaucug gccauccaua gcauauuuaa gaguagaggc      480

cacuuccucu uugaggggca gaacuuaaaa gagaucaaga auuucgaaac acuguacaac      540

aaucuuauug cguucuugga ggauaacgga aucaacaaau ccauugacaa ggauaaucug      600

gaaaagcuag agaaaaucgu gugugacucu ggcaaggguc ucaaagacaa ggaaaaagag      660

uuuaaggaga uauucaacag cgauaagcag cuggucgcua uuuuuaaacu uuccgugggg      720

cugucaguua guuugaauga ccucuucgau acugacgaau acaagaaagg agagguggaa      780

aaggagaaaa ucagcuuuag ggaacaaauu uaugaggaug acaagccaau cuacuauucu      840

auccugggcg agaagauuga auuacuggau auagccaaau ccuucuacga cuuuaugguc      900

cucaacaaua uccuugcaga uucacaguau auuagcgagg ccaaggugaa acuguacgag      960

gagcauaagc gggacuugaa aaaccuaaag uauaucauac guaaguacaa ucgcgaaaac     1020

uacgauaaac uguucaagga caaaaaugag aguaacuauc cggcuuacau uggccucaau     1080

aaggagaaau cuaagaaaga aguaaucgag aaguccaagc ugaaaaucga cgaucuuauu     1140

aagagcauca aaggguaucu gcccaagguu gaagagauug aggaaaaaga caaggcaaua     1200

uuuaacgaga ucuugaauaa gauugaacuc aaaaucaucc ugccuaagca gagaauuucg     1260

gauaacggaa ccuuaccaua ccagauacac gaggccgaac uggagaaaau ccuagagaau     1320

caaucuaagu auuacgacuu ccucaacuau gaagagaacg guauuaucac aaaagauaag     1380

cugcuuauga ccuuuaaguu caggauuccc uacuacgugg gcccucuuaa uuccuaucau     1440

aaagacaaag ggggaaacuc auggaucguc cggaaagaag agggcaagau acugccaugg     1500

aauuuugaac agaaagugga uaucgagaag agugcugagg aauuuauuaa gcgaaugacu     1560

aacaaaugca cauaccucaa uggugaggac gugaucccca aggauagcuu ccuguauucc     1620

gaauacauua uccuuaacga gcugaauaaa guccagguua acgacgaauu uuugaaugag     1680

gagaacaagc gcaaaauaau ugaagaacuc uucaagggga auaaaaaggu gucugaaaag     1740

aaauuuaagg aguaucuguu aguaaaccag aucaccacgg gaacuauuga gcugaaaggc     1800

aucaaggaua gcuucaauuc aaacuacaua aguuauauca aauuuaagga cauuuucggc     1860

gaaaagcuaa aucucgauau cuacaaagag auuuccgaaa agagcauccu guggaaaugu     1920

cuuuacgggg acgacaagaa aauauuugag aagaagauua aaaacgaaua uggagauauc     1980

uugaccaagg acgagaucaa aaagauuaac acauucaaau uuaauaacug ggguagacug     2040

ucugagaagc uccugacugg caucgaguuc auuaauuuag aaaccgggga augcuacuca     2100

agugugaugg aggcccugag gcggacaaac uauaaucuua uggaauugcu cagcuccaag     2160

uuuacccugc aagagaacau agauaaugag aacaaagaca ucucucaguu cucguaccgc     2220

gaucuagucg aagagagcua uguguccccu ucacugaaga gagcgauucu ccagacgcug     2280

aaaaucuacg aagagauuaa gaaaaucacu ggaaggguuc caaagaaggu guuuauagaa     2340

auggcacgug gcggugacga gaguaugaaa aauaagaaaa ucccggcccg gcaagagcag     2400

cuuaagaaau uguaugauuc uugugggaac gacauugcua auuucagcau cgauauuaag     2460

gaaaugaaau ccucacugag cuuauacgac aacaauucuc uccgacagaa gaagcuguac     2520

cuuuauuacc ugcaguucgg caaaugcaug uauacaggaa gagagaucga uuuggacagg     2580

cuccugcaaa acaaugauac cuacgacaua gaccacauuu auccccgcuc caaggucauc     2640

aaagaugaca guuuugauaa ccuagugcug guacucaaga acgaaaaugc cgagaaaagc     2700

aacgaguacc cuguuaagaa ggaaauccag gagaaaauga agucuuucug gcgguuucug     2760

aaagaaaaga auuucauuuc cgacgagaaa uauaagagac uuacuggcaa ggaugacuuu     2820

gaauugaggg gguucauggc acgccagcug gugaacgucc gacagacaac caaagaggug     2880

ggaaagauau uacaacagau cgagccagaa auuaaaaucg ucuacucaaa ggcugagauu     2940

gccaguagcu uucgggaaau guucgauuuu aucaaagugc gugagcucaa ugacacgcac     3000

caugcgaagg augccuaccu gaacauaguu gcagguaaug uguauaacac caaguucaca     3060

gaaaaacccu acagauaucu ucaggagauc aaggagaauu acgacguaaa gaagauuuau     3120

ucguacgaua ucaaaaacgc uugggacaag gaaaauuccc uggagauugu caaaaagaac     3180

auggaaaaga auacugugaa caucaccagg uuuauaaaag aggaaaaggg ccagcuauuc     3240

gaccucaauc cuauuaaaaa gggggagaca ucuaacgaga ucaucagcau uaaaccaaag     3300

guuuauaaug gaaaggauga caaacugaac gaaaaguacg gcuacuauaa aucauugaac     3360

cccgccuacu uccuguaugu ggagcacaag gagaaaaaua agcgcaucaa gaguuuugaa     3420

cgggucaacu uaguggaugu aaauaacaua aaagacgaga agucccucau uaaauaccug     3480

guggaaaaua agaaacuugu ugagccgaga gucaucaaga agguguauaa aaggcaagug     3540

auucugauca acgauuaccc uuauucuaua gucgcuuugg acagcaauaa gcucauggau     3600

uucgaaaacc ugaaaccccu auuucuggag cgaaaguacg agaaaauucu uaagaauguu     3660

aucaaauucc ucgaagacaa ccaggguaag uccgaggaaa auuacaaguu uaucuaucug     3720

aaaaagaaag aucgcuacga gaagaacgaa acuuuggagu cagugcggga aagauauaau     3780

auugaguuca acgagaugua cgacaaauuu cuggaaaagu uaaauagcaa ggauuauaaa     3840

aacuacauca acaauaagaa auaucaggac cuccuggaug ugaaggagaa auucauuaag     3900

uugaaccugu uugacaaggc cuucaccuug aaaaguuuuc ucgaccuguu caauaggaag     3960

acaauggcag auuuuucuaa aguagggcua accaaguacc ugggcaaaau acagaagauc     4020

uccagcaacg uccuuucgaa gaaugaacuc uaccuguugg aggaaucagu gacuggauua     4080

uucguuaaaa agauugagcu guga                                            4104


<210>  3101
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3101
auggugaaga aaaaguacgc caucggcauu gacauaggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa aguacccucu aagaaaauga agguguucgg uaauacugag      120

aagagauaca ucaaaaagaa ccugcucggc acgcuucugu uugaugaagg gaauaccgcu      180

gagaacaggc gguugaaacg cacagcacga cguagauaua cuaggcggcg caauagaauu      240

uuauaccugc aggaaaucuu cgccgaggaa auuaacaaga ucgacgauuc auuuuuccaa      300

aggcuagacg auaguuuucu cauagucgag gacaaacagg gaagcaagca cccuaucuuc      360

ggcacccugc aggaggaaaa ggaguaucau aaacaguuuc caacaauuua ccaccuucgg      420

aagcaacugg cggauuccuc ucagaaagcu gacauccgcu ugauuuaucu cgcccuggca      480

cauaucauaa aguaccgagg ucacuuccug uuugaagggg aucucaaauc ggagaauaag      540

gacgugcagc auuuauucaa cgauuuuguu gagauguucg acaaaaccgu ggaaggauca      600

uaccugagcg agaaucuucc gaacgucgcc gacguguugg uagaaaaggu uaguaagucc      660

agaaggcugg agaauauucu acacuauuuu cccaacgaaa agaagaaugg ccucuucggc      720

aacuuccuga cucuugcucu gggguugcag ccuaauuuua aaacaaacuu cgagcucgca      780

gaggaugcca agauccaauu uucuaaagaa accuacgagg aagaccugga ggaauuacug      840

ggaaagauug gugaugacua ugcugaucuc uucaucgcca cgaaaagccu uuacgacggc      900

auccuguugg cggggauucu auccacuacc gauucaacaa cuaaagcacc acugaguagc      960

ucuaugguga accgguauga ggagcaccag aaggaccucg cccugcuuaa aaauuuuaua     1020

caucagaacc uguccgauuc auacaaggaa gucuucaaug acaaauugaa ggauggauau     1080

gcuggcuaca ucgaggguaa gaccacacag gaaaacuuuu accguuucau uaaaaaggcc     1140

aucgagaaaa uagaagggag caauuauuuu auugacaaga ucgaccgcga ggauuuccuc     1200

agaaaacaaa ggaccuuuga caacggaagu aucccccacc agauucaucu gcaggagaug     1260

cacgcaauca uucggcgaca agcugaauuu uacccuuuuc ugguggagaa ucaggauaag     1320

auagaaaaga uccugacguu ccgcauucca uauuacgugg gcccccuagc cagaggcaaa     1380

ucugaguucg cguggcucaa caggaagucc gacgagaaaa uccggccuug gaauuuugau     1440

gaaauggucg acaaggagac uagcgccgaa aacuucauca cacgcaugac caauuaugau     1500

caguaccugc cagaccagaa aguucuuccg aagcacucgu ugcuguauga gaaauuugca     1560

guguacaacg aacucacuaa gguaagauac gugacagagc aagggaaguc uuucuuuuuc     1620

gaugcuaaua ugaaacagga gauuuuugac ggagucuuca agguguauag gaaaguuacc     1680

aaggaaaaac ugauggauuu ucuugguaag gaguucgacg aauuucguau aguggaucug     1740

uugggccucg acaaggacaa caaauccuuc aaugccucac uggggacgua ccaugauuua     1800

aagaaaaucg ucaguaagga ccugcuagau aaccccgaga acgaagacau ucucgagaau     1860

gugguacuga cccuuacauu guuugaggau cgggaaauga uccgaaaaag acuggagaag     1920

uauaaggacg uucucacuga ggaacagagg aaaaagcugg aacgccggca cuacaccgga     1980

uggggcagau uaagcgcuaa acugauuaac gguaucaggg auaaggugac acgcaaaacu     2040

auacuugacu auuugaucga ugacgggacc uccaaucgaa acuucaugca gcucauuaau     2100

gaugacacac ugucuuucgu cgaugagauc cggcuagccc aaggcagcgg agaggcagaa     2160

gacuaccgug ccgaggugca gaaccuggcu ggcaguccug cgauuaagaa ggggauccuc     2220

cagucacuga aaauagucga cgaacuuauu gaggugaugg gauaugaucc agagcauauc     2280

guuguggaaa uggcaagaga gaaucaguuu accaaccaag guaggcgcaa uucccagcag     2340

cgguacaaga aaauugaaaa cgccaucaag aauuuggaca gcaacuuaaa uucuaaaaua     2400

uuaaaggagu accccacuaa caaucaggcu cuccaaaacg auagacuguu ccuuuauuac     2460

cugcagaacg gcaaagacau guauacggau gaagaguugg acaucgauca gcucucacaa     2520

uacgacauug aucacaucau uccucaggcc uuuaucaagg acgauagucu ggacaauaag     2580

guacuaacaa aaagcgcaaa gaacaggggg aaauccgacg augucccauc ucuggagaua     2640

gugcauaaga aaaagaauuu cuggaagcag cuccuggacu cgcagcuuau uagccaacga     2700

aaauuugaua acuugaccaa ggccgaacgc ggaggccuga cuaaugagga caaagcucgg     2760

uucauccaga gacaguuagu ugaaaccagg cagaucacaa agcacguggc ccguauucuc     2820

gauacccgcu uuaacacuaa acuggacgag gcagguaauc ggaucagaga ucccaagguc     2880

aacauuauaa cacuuaaguc caaucuggug ucacaauuca ggaaagacua ucagcuauac     2940

aagguacgag aaaucaacaa uuaucaccau gcgcacgaug cuuaccucaa cgccgugguu     3000

gcaacggccc uguugaaaaa guacccgcag cuggcuccug aguuugucua uggggacuac     3060

cccaaauaua auaguuacaa gucucgcaag agcgccaccg agaaaguguu auucuauucc     3120

aacauuauga auuucuuccg gagagugcuc gucuacucaa agacuggcga aguuaggauc     3180

cggccaguga uugaggugaa caaagaaaca ggagagaucg uaugggauaa gaaaagcgac     3240

uuccgaaccg ucagaaaggu gcugucuuau ccucagguua acguggucaa gaaaguggaa     3300

augcaaacag gcggguuuuc caaggagagu auacuucagc auggagauag cgacaaacug     3360

aucccccgca agacugagaa auucuacuug gacaccaaga aauacggugg cuuugauucu     3420

ccaacgauug cauauuccgu ucuccugauc gcugacauug aaaaggggaa ggccaaaaag     3480

cuaaaaaggg ugaaggagcu gaucggaaua accauuaugg aacguauggc guucgagaaa     3540

aaucccaucg aguuucuuga acacaagggc uacaagaaca uccucgagaa aaauauuaua     3600

aagcugccua aauauucauu guucgaacug gagaacgguc ggagaagguu acucgccagu     3660

gcuaaggaac ugcagaaagg gaaugagaug auccuuccac cgcaccuggu aacauugcuc     3720

uaccauagcu cgaacauuca caagaucacu gagcccauuc aucugaauua ugucaacaag     3780

aauaaacacg aauuuaagga gcuacugcgc cauaucuccg auuucucuac ccgauacaua     3840

cuugcacaag acagacucag caaaaucgag gaacuguaug auaagaacga cggcgaugac     3900

auuucagauu ugacaaguuc cuuugugaau uuacugacuu ucaccgccau cggagcuccu     3960

gccgcauuua aauuccucgg cucugugauu gaccggaaga gguacacgag caucgcggaa     4020

auacuggagg ccacacuuau ucaccagucc gucaccgggc uguacgagac ucgcaucgau     4080

uugucaaagc uaggaggcga cuga                                            4104


<210>  3102
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3102
auggugaaga aaaaguacgc caucggcauu gacauaggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa aguacccucu aagaaaauga agguguucgg uaauacugag      120

aagagauaca ucaaaaagaa ccugcucggc acgcuucugu uugaugaagg gaauaccgcu      180

gagaacaggc gguugaaacg cacagcacga cguagauaua cuaggcggcg caauagaauu      240

uuauaccugc aggaaaucuu cgccgaggaa auuaacaaga ucgacgauuc auuuuuccaa      300

aggcuagacg auaguuuucu cauagucgag gacaaacagg gaagcaagca cccuaucuuc      360

ggcacccugc aggaggaaaa ggaguaucau aaacaguuuc caacaauuua ccaccuucgg      420

aagcaacugg cggauuccuc ucagaaagcu gacauccgcu ugauuuaucu cgcccuggca      480

cauaucauaa aguaccgagg ucacuuccug uuugaagggg aucucaaauc ggagaauaag      540

gacgugcagc auuuauucaa cgauuuuguu gagauguucg acaaaaccgu ggaaggauca      600

uaccugagcg agaaucuucc gaacgucgcc gacguguugg uagaaaaggu uaguaagucc      660

agaaggcugg agaauauucu acacuauuuu cccaacgaga aaaagaaugg ccucuucggc      720

aacuuccugg cucuugcacu gggguugcag ccuaauuuua aaacuaacuu cgagcucgcc      780

gaggaugcua agauccaauu uucuaaagaa acauacgagg aagaccugga ggaauuacug      840

ggaaagauug gugaugacua ugccgaucuc uucaucgcga ccaagagccu uuacgacggc      900

auccuguugg cagggauucu auccacgacu gauucaacca caaaagcccc acugaguagc      960

ucuaugguga accgguauga ggagcaccag aaggaccucg cucugcuuaa aaauuuuaua     1020

caucagaacc uguccgauuc auacaaggaa gucuucaaug acaaauugaa ggauggauau     1080

gccggcuaca ucgaggguaa gacuacccag gaaaacuuuu accguuucau uaaaaaggca     1140

aucgagaaaa uagaagggag caauuauuuu auugacaaga ucgaccgcga ggauuuccuc     1200

agaaaacaaa ggacauuuga caacggaagu aucccccacc agauucaucu gcaggagaug     1260

cacgcuauca uucggcgaca agccgaauuu uacccuuuuc ugguggagaa ucaggauaag     1320

auagaaaaga uccugaccuu ccgcauucca uauuacgugg gcccccuagc gagaggcaaa     1380

ucugaguucg ccuggcucaa caggaagucc gacgagaaaa uccggccuug gaauuuugau     1440

gaaauggucg acaaggagac gagcgcagaa aacuucauca cucgcaugac aaauuaugau     1500

caguaccugc cagaccagaa aguucuuccg aagcacucgu ugcuguauga gaaauuugcu     1560

guguacaacg aacucaccaa gguaagauac gugacugagc aagggaaguc uuucuuuuuc     1620

gaugccaaua ugaaacagga gauuuuugac ggagucuuca agguguauag gaaaguuaca     1680

aaggaaaaac ugauggauuu ucuugguaag gaguucgacg aauuucguau aguggaucug     1740

uugggccucg acaaggacaa caaauccuuc aaugcuucac uggggaccua ccaugauuua     1800

aagaaaaucg ucaguaagga ccugcuagau aaccccgaga acgaagacau ucucgagaau     1860

gugguacuga cgcuuaccuu guuugaggau cgggaaauga uccgaaaaag acuggagaag     1920

uauaaggacg uucucacaga agaacagagg aaaaagcugg aacgccggca cuacacugga     1980

uggggcagau uaagcgccaa acugauuaac gguaucaggg auaaggugac ccgcaaaaca     2040

auacuugacu auuugaucga ugacgggacu uccaaucgaa acuucaugca gcucauuaau     2100

gaugacaccc ugucuuucgu cgaugagauc cggcuagcac aaggcagcgg agaggccgaa     2160

gacuaccgug cugaggugca gaaccuggcg ggcaguccug caauuaagaa ggggauccuc     2220

cagucacuga aaauagucga cgaacuuauu gaggugaugg gauaugaucc agagcauauc     2280

guuguggaaa uggccagaga gaaucaguuu acaaaccaag guaggcgcaa uucccagcag     2340

cgguacaaga aaauugaaaa cgcuaucaag aauuuggaca gcaacuuaaa uucuaaaaua     2400

uuaaaggagu accccaccaa caaucaggcc cuccaaaacg auagacuguu ccuuuauuac     2460

cugcagaacg gcaaagacau guauacugau gaagaguugg acaucgauca gcucucacaa     2520

uacgacauug aucacaucau uccucaggca uuuaucaagg acgauagucu ggacaauaag     2580

guacuaacga aaagcgccaa gaacaggggg aaauccgacg augucccauc ucuggagaua     2640

gugcauaaga aaaagaauuu cuggaagcag cuccuggacu cgcagcuuau uagccaacga     2700

aaauuugaua acuugacaaa ggcugaacgc ggaggccuga ccaaugagga caaagcccgg     2760

uucauccaga gacaguuagu ugaaacuagg cagaucacca agcacguggc acguauucuc     2820

gauacacgcu uuaacaccaa acuggacgag gcggguaauc ggaucagaga ucccaagguc     2880

aacauuauaa cucuuaaguc caaucuggug ucacaauuca ggaaagacua ucagcuauac     2940

aagguacgag aaaucaacaa uuaucaccau gcucacgaug ccuaccucaa cgcagugguu     3000

gccacagcuc uguugaaaaa guacccgcag cuggccccug aguuugucua uggggacuac     3060

cccaaauaua auaguuacaa gucucgcaag agcgcaacgg agaaaguguu auucuauucc     3120

aacauuauga auuucuuccg gagagugcuc gucuacucaa agaccggcga aguuaggauc     3180

cggccaguga uugaggugaa caaagaaacu ggagagaucg uaugggauaa gaaaagcgac     3240

uuccgaacag ucagaaaggu gcugucuuau ccucagguua acguggucaa gaaaguggaa     3300

augcaaaccg gcggguuuuc caaggagagu auacuucagc auggagauag cgacaaacug     3360

aucccccgca agacagagaa auucuacuug gacacuaaga aauacggugg cuuugauucu     3420

ccaaccauug cuuauuccgu ucuccugauc gccgacauug aaaaggggaa ggcgaaaaag     3480

cuaaaaaggg ugaaggagcu gaucggaaua acgauuaugg aacguauggc cuucgagaaa     3540

aaucccaucg aguuucuuga acacaagggc uacaagaaca uccucgagaa aaauauuaua     3600

aagcugccua aauauucauu guucgaacug gagaacgguc ggagaagguu acucgcuagu     3660

gcaaaggaac ugcagaaagg gaaugagaug auccuuccac cgcaccuggu aaccuugcuc     3720

uaccauagcu cgaacauuca caagaucaca gagcccauuc aucugaauua ugucaacaag     3780

aauaaacacg aauuuaagga gcuacugcgc cauaucuccg auuucucuac ucgauacaua     3840

cuugcccaag acagacucag caaaaucgag gaacuguaug auaagaacga cggcgaugac     3900

auuucagauu ugaccaguuc cuuugugaau uuacugacau ucacugcuau cggagccccu     3960

gcagcguuua aauuccucgg cucugugauu gaccggaaga gguacaccag caucgccgaa     4020

auacuggagg cuacgcuuau ucaccagucc gucacagggc uguacgagac ccgcaucgau     4080

uugucaaagc uaggaggcga cuga                                            4104


<210>  3103
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3103
auggacaaga aauacagcau cggccuggau auugggaccu ccucuauagg auucgugggu       60

gucgacgaga acggcuggcc ccucaauauc aaggggaaaa gagccauugg agcucaccuu      120

uuuagggaag gcgaggcagc cgcggaaaca cgggguuucc gcacuacgcg acguagauau      180

aacaggcgga aguggcgccu gagauuguua agggagaucu uugaugaacc uauuucacgg      240

guugacccaa ccuucuuugc ucgcaugaaa gagaguagcg uguccccgaa ggaucccaau      300

uacgacgggc uggccaagga gauccuaaua ccucgagaua aagacggaaa gauccucuau      360

ccaacaauuu accaucugag acacgcacuu auguaugaag aucaucaguu cgacaucagg      420

gagauuuacc uggccaucca ccauauagua aaauaucggg gccacuuuuu gcucgauggc      480

gcugcaaaga acuucgaauc uucgaaucug gaccugaaau cagauauuca cgagaucaac      540

gccauucucg uggggaucuu uggaaaugag caaguccagu ucaacgacca guacguggaa      600

aaguuaaagg agguucugcu ugacagcacu cagagucguu ccgaucgcca aaaagcugcc      660

gcgaaggugu ugcugcccac caaugaccua gauaaagaca caaccaagau ccucaaaacu      720

gucucuacag aaauuaccaa ggcaauaacg ggucugaaag ccaaguuuuu cgugaucacu      780

ggcaacgaua uugcuaagga ccagcagaaa gauuggaccu uuagcaucga cacacuugau      840

gagaagaugu ccguaauaga accuucauuc agugagaaaa auuucagccu gauuacuauc      900

uugcagaagc ucuacucuuc cguuacccug gccgggaucg ugccaaacgg auuaucacug      960

agcgaggcaa ugauucaaaa auaugacaga caugcuagug aucucguccu ucugaagaag     1020

uacugggaca caaaacccaa gguguugaaa caccagcuac agaauaccua ugccgaauac     1080

aucgagauuc aucagguggu cgacgaauuu uauucugauc uguccaagcu cauuaaaaac     1140

gacgaugaca cgcuggcgca agagaucaag agcaagauua cuaucggcaa uuuccuuccu     1200

aaacagagga caaccgaaaa cgguguuauc ccauaccagc ugcaacagcg ggaguuggau     1260

acuauuauaa agaaccagca ggaguauuac ccguggcucg ccgaagagaa ucccguggca     1320

gaccaccaaa ucgcuuuucc uuacaaacug gaugaauuag uaggguuccg aauaccauau     1380

uacgugggac ccaugaucgc caaggaggac acaucgaacu cuaaaaauuc caaguuugcu     1440

uggaugauuc gcaagcaguc aggcgaaguc accccuugga acuucgauga gaaaaucgac     1500

agaacggcca gugcaaccgc cuuuauagau aggaugacaa cuaccgacac auaucugauc     1560

ggcgaggaug ugcuaccaaa gcagagccuc auuuaccagc gguucgaagu ucugaaugag     1620

cuuaacaaaa ucuccauuga cgggcaaccc aucacucgca agcagaaaca gucuguguau     1680

acccacgucu uugaaaagaa aaagacagug cagguaaagg acguucaaaa auucuuggug     1740

agcucaggag aggucaugag ucugccgaag auagguggcc ucgcuaauga gaaacaguuu     1800

aacuccagcc ugucuacccu uuacgauuau aauaagauua ucccugacgc gcuggaagau     1860

agaaacaaag uggacgauau ugagaagauc auauuguggu caacuaucuu cgaagacaau     1920

aguauuuuug ucgagaagcu cgauagcauc gacuggcuga cguccucuca gaggaaacgu     1980

uuagcacaga agcgguaccg agggugggga agacugucgc uaaggcuccu gacaaccuuc     2040

aaaaacaagg auggcuacag cauucuugac gaauugugga acuccaauca uaauuucaug     2100

gagauccaag cccagucaga uuuuagugag cagauagcua cugccaacaa caauaaccug     2160

ucucaagugc uccccgcaaa augggaacca ggucgcaaga aucuggccag cuuagacgag     2220

uucauuucca accuguauac cucaccucag aauaagaaag cucuacggaa ggccaucaga     2280

guugugcagg auaugaugac agcagcgggg cacaugcccg agaaaaucuu uauugaagua     2340

gcuagggaca ccagcaacuc ucaucagcaa acuaucgccc gccagcgaca guugcagcaa     2400

cucuaccagu ccgaagcaaa ggaggucauu gacaguagcg ugcggucuga gcuggaugac     2460

auacuuaaaa aucgugccaa caucaaggau cagcuguucc ucuauuuuca gcaaggcgga     2520

agagaccugu acacaggcaa ggcuauuaau aucgauaaac uuuccuugua ucacauugac     2580

cauauccugc cacagucauu aguuaaggau gacagucucg auaacagggu gcuggucagc     2640

cagaaaacga augaagacaa gggggugacc uucguaucgg auaaauacau gaacaauaug     2700

cuucccacuu ggaagcgcug gcacgagaug ggacugauau ccgcccggaa auugaagaac     2760

cucacaauga gaccugacga aaucgacaag uaugcaccag guuuuauuca aaggcagcug     2820

guggagaccc gacagguuac aaaacuuguc aucgaacugc ucgaugcucu gcuuggcaau     2880

ucucagacug agauuaucac cauaaaggcc agcuugucac accaauuccg ccagcaguuu     2940

gaccugguga aaauucggga ggugaacgau aaccaucacg cguucgacgc cuuuuuagcu     3000

gcaagaaucg ggacguaccu cuacaagagg uauccgaaac ugcgucccua cuucgucuau     3060

ggacaguaca ugcaaaauca uaagaaccuu aagaguauca aucugauuaa aacccuagau     3120

uccgacaagg ccauaguuga ugaaggcaca ggugagaucc ucugggacaa aaagucugau     3180

cuggcuaaaa ugauuaagau cuauaacuac cagcgcuugc ugauuacuca cgaaagccgg     3240

accuccuuug gggccaugua uaagcagaaa aucuuaaagg caacagacgc gaaacagaau     3300

ggcggaggcu ucaagcucgu gccuacuaaa agaaacuggc caguggauau auacggggga     3360

uacaccgguc aaacggccgc uuaucugaca aucguaaggg ucuacucaaa gaagaccagc     3420

gagcuucgag ugcugcccau uuauacuaau aaaguucagc agaucgagaa ggccaugaca     3480

cagggcgaca aagcaaccau guuggcuuuc aagaaaguga uugccccuca acucgggaag     3540

gaaaacaaga aaacucacga gaucaagaua ccagaauuug augucgugau ucccaaaguu     3600

cugcuagacc agcuggugau cgacgauauc gcaggaaguc cgcaucgguu ccgccuuggc     3660

accgacacau acuaucacaa uucucagcaa cucuuucugc cuuuguccau gcagagacag     3720

cugauggaua gggagagcga cgauaaggua uacuuagaca uuuucaacga uguccucaaa     3780

cagguggacu cguauuuuaa ucuguacgau gugaacgguu uccgggccgc ucuacaaaag     3840

ucuaaaaagg cguuugaaca gcugccaauc aaugacuucc ccaaggaugu cccugacaac     3900

aaaaagaaaa aggggaaacg aucaauauug aaggagauuu uuaucggccu ccaugccaau     3960

gcaacgcugg gaaaccuaaa gcagaugggc auuaaaaccg auuucgggaa gcugcagaaa     4020

aguaacggac uuagaaucac ugagaauugc aagauaauuc accaaucccc cacaggccuc     4080

uuugaacgca ucguuccaau caaauga                                         4107


<210>  3104
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3104
augagcagac ccuacaacau cggccuggac auugggaccu ccucuauagg auggucagug       60

gucgaugacc agaguaagcu cguuagcgug agggguaaau auggcuacgg gguacggcuu      120

uaugaugagg gacaaacagc cgcugaacgc cgauccuucc guacuacgag aaggcggcug      180

aagcgcagaa aauggagguu gggcuuacug cgggagaucu uugaaccuua cauuacccca      240

guggacgaua cauucuuucu acgccagaag cagucuaauc ucucgccgaa agaccagcga      300

aagcuguauc cccaaacuuc acuuuucaac gauagaaccg acagggcauu uuacgaugac      360

uauccuacaa ucuaccacuu gcgguacaag uugaugaccg agaaacguca guucgauauu      420

cgcgaaaucu aucucgccau gcaucacaua gucaaguaca gaggucauuu ucugaaugag      480

gcgccaguga gcaguuucaa auccucugag aucaaccugg uugcucacuu ugacaggcuc      540

aauacuauuu ucgccgauuu auuuagcgaa uccggguucc agcuggagac agacaaacuu      600

gcagaaguga aagccuugcu gcuagacaac cagcaaucag cuaguaaucg gcagcgacag      660

gcacucagcc ugaucuauac ccccucuacg aacaaggccg ucgagaagca gaauaaagcu      720

auugccacug agcuucugaa ggcgaucuug ggacucaaag caaaguuuaa cgugcugacc      780

ggcauagaag ccgaggaugu aaaggcuugg acauuaacuu ucaaugccga aaacuucgac      840

gaggaaaugg uuaagcugga guccucacuc gaugacaaug cacaucaaau uaucgagagc      900

cuucaggaac uguacagugg cguguugcua gcugggauug ucccugagaa ccagucucug      960

ucccaagcca ugaucaccaa auaugaugac caccagaagc accucaagau gcugaaagcg     1020

gugcgcgaag cccuugcacc agaggauaga cagaggcuga agcaggcuua cgaccaauau     1080

guggauggac aggaaaauac aaaagccuac agcaaggagg acuuuuacgg ugauaucacc     1140

aaggcuuuga aaaacaaccc cgaccauccu auugucucgg agauaaagaa acucaucgaa     1200

cuggaccagu ucaugccaaa gcagcggacg aaagauaaug gcgccauucc gcaccaauua     1260

caucagcagg agcuggaccg caucauagaa aaccagcaac aguauuaccc cuggcuagca     1320

gagcucaauc cuaacucuaa gagacagacu guugccaaau auaagcugga ugaacuugug     1380

gcuuuuaggg uaccauacua uguggggccc uugauuacag cggagcagca acgucagucc     1440

ucagacgcaa aguucgccug gcugauccgg aaagcugagg gacgaaucac cccuuggaau     1500

uuugaugaca aggucgauag acaggccagu gcaaacgagu ucauuaaaag gaugacuaca     1560

accgacacgu accuccuggc cgaggaugug cuuccaaagc aaagccugau cuaucagcgc     1620

uuugaaguuu ugaaugagcu caacggccug aaaauugacg aucagcccau aaccacagag     1680

uuaaagcagg cuaucuucac ugaccuguuu augcaaaaga uuuccgugac cgucaaaaau     1740

auccaggauu accuaguguc ugaaaagcgg uacgccagca gaccggcaau cacaggucuc     1800

ucagacgaga acaaauucaa uaguaggcug uccacuuauc acgaccuuaa gaugauugua     1860

ggggaugcgg uugacgaugu ggacaaacag gcugauuugg aaaagugcau agaguggagc     1920

accaucuuug aagacggaaa aauuuacucu gccaagcuga acgagaucga uuggcucaca     1980

gaccagcaac gcguccagcu ggcagccaag cgauaucggg gcuggggccg uuuaucagcu     2040

aaacugcuua cccagauugu gaaugccaac gggcagagaa ucauggauuu gcucugggac     2100

acuacggaua auuucaugag gauaguccac agugaggacu uugauaagcu gaucacagaa     2160

gcaaaccaaa ugaugcuagc ugagaacgac gugcaggacg uuauuaauga ucuguacacc     2220

agcccucaga acaaaaaggc ccuccgccaa auccugcuug ugguaaauga cauucagaaa     2280

gcgaugaagg gacaggcccc cgaacggauc uugauagagu ucgcuagaga agaugagguc     2340

aacccaaggc uguccgugca gcgaaagcgc caaguugaac agguguauca gaauauuucu     2400

aacgaauuac ucaauaacac ugagauccgg aaugaacuga aagaccuuuc gaacagcgca     2460

cuguccaaua ccagauuguu ccucuacuuu augcagggug gcagggauau guauacaggg     2520

gacucacuga acauugaucg ucuaaguacc uacgacaucg aucauauacu gccucaaucu     2580

uucgucaagg acaauagccu cgauaaccgc gugcugguau cccagaaaau gaaucgguca     2640

aaggccgacc aggugcccac ugacuuuaca agcguugagc uuggcaaaaa gaugcaguug     2700

caaugggaac agaugcugag agcuggauua aucacgaaga aaaaguacga uaaccucacc     2760

cugaacccag accacauuuc uaaauaugcc augaaggguu ucaucaauag gcagcuuguc     2820

gagacucgac aggugauuaa acuggcaaca aaccuacuca uggagcaaua cggggaagau     2880

aauaucgagc ugauaaccgu gaaguccggc uugacacauc agaugcggac ugaauuugac     2940

uuccccaaga accgcaaucu gaacaaucac caucacgcgu uugaugccua uuuaaccgcu     3000

uucgucggac ucuaccugcu uaaaagauau ccuaagcuga aaccauacuu uguuuauggc     3060

gaguaccaga aggccaguca acaggacaaa uggaggaacu ucaauuuucu gaacgggcuc     3120

aagaaagaug agcuggugga cgaaaauacg gaggcaguga uuugggauaa ggaaagcgga     3180

cuagcuuacc ugaacaagau cuaucaguuc aaaaagaucc uuguaacccg ggagguccac     3240

gaaaauucug gugcccucuu uaaccagaca cuguacgcag ccaaagacga uaaggcuucc     3300

ggccaagggg gaaaacaguu gauuccggcg aagcaggacc gacccacugc ccuguauggc     3360

gguuacucag ggaagaccag ugcauauuua ugucucguga aaaucaagaa uaaaagcgag     3420

uacauuuaua gaauauucgg cguuaacaca ucgugguccu cugagcugaa gcagauccaa     3480

gauccugcug aacgccuugu gaggcuggag aaauugcuca cuccacagcu gaccauuaac     3540

aagaagaaaa agggagaacc cgucccugug acgaaagacu ucaagaucau ucuaccacgu     3600

auccugauaa aucagaaauu uuacagccgg ggccaggaca ucucaauugc cagugaguuc     3660

uaccuucaua acgaacaaga gcucguucug gauaaguccg acaucaagau uuugaauggg     3720

gccaucaaag ugaacaauuc uaacaagcag guaauaaauu uauuuaacca cauucuggag     3780

caggucaauc aauauuuccc cguguacgau aucuauaaaa gcagagaagu gcucaacucc     3840

ucaaaggaga ucuuugaaaa ucugacaugg aacgaccagu ggaggaaucg caaacuuauu     3900

aacgucggac agaagaccau acugaaucga aucuugauug gucuacaugc uaacgcagcc     3960

agaaucaaga uuagcgagcu gggcuucaaa aaugauuuug ggcuccugac ucagacaagu     4020

ggaaucaccu ucacuccgga cacccaaaua aucuaccagu cuccuacagg ccuuuuugaa     4080

cggaggguug cguuacgcga ucuguga                                         4107


<210>  3105
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3105
augaagagaa gcuacaacau cggccuggac auugggacca auuccauagg augggccauc       60

guggaugacc agucuaaacu cauuucaguc aaggguaaau auggcuacgg gguuaggcuu      120

uaugaugagg gacaaacagc ugcagaacgg cgcaguuucc gaacuacgcg uagaaggcug      180

aagcggcgca aauggagauu gggcuuacug agggagaucu uugaacccua cauuaccccu      240

aucgacagca cauucuuucu acggcagaag caguccaacc ucucuccaaa ggaucagcgc      300

aaacuguauc cgcaaacuuc gcuuuucaau gaccgacccg augccgacuu uuaccagcag      360

uauccuacca uauaccaccu gagauaccag uugaugacag agaagaggca auucgauauc      420

cgggaaauuu aucucgcgau gcaucacauc gugaaauacc guggucauuu ucugaacgag      480

gcuccaguag ccucauucaa gagcagugag auuaaucugg cagaacacuu ugaccagcuc      540

aacaccaucu ucgccgauuu auuucgcgag uccggguucc agcuggaaac ugacgagcuu      600

guggcuguca aagcauugcu gauggauaau cagcaaucug ccagcaacag acagaggaag      660

gcucuaccca agcucuauac accuuccgcc gacaaaucaa uagagaagca gcggaaagcg      720

auugcaaccg aaauccugaa ggccauucuu ggacugaaag cuaaguuuaa ugugguuacg      780

ggcaucgagg ccgaagacgu gaaagcaugg gcuuugacuu ucaacgccga gaauuucgau      840

gaagaacucg cgaagcugga gaguagcuua gacgauaacg gccaugaagu caucgggauu      900

cugcaagagc ucuacucugg aauacuucug gccgguaucg ugccagaaaa ucaguccuug      960

ucacaggcaa ugauuaccaa guaugaggaa caccaggcuc accuaaaaau gcugaaggcc     1020

guacgagacg aucucgcuac agccgaccaa cagaaacuga agaaagcaua cgaucaguau     1080

aucgacggcc aggagaacac uaaggccuac agcaaggagg cuuuuuacga ugacguuacc     1140

aaagcgcuua aggauuauag ugaacaaccc cuggugucug agauacagaa auugauugaa     1200

cucgaccagu ucaugccuaa gcagcgcaca aaagacaaug gggcaauccc acaucaacug     1260

caccagcagg aguuagauag aaucauugaa aaccagcaac aguacuaucc guggcuggcc     1320

gagcuaaacc ccaauccuaa gaggcagacc gucgcuaagu acaaacucga cgagcuggug     1380

gccuuucggg ugccauauua cgucggaccc cuuaucacgg cagaacaaca gcagcagucc     1440

agcaacgcca aguucgcuug gaugauucgc aaaacugagg gcagaauaac accuuggaau     1500

uuugaugaca agguugauag gcaagccucg gcaaacgagu ucaucaaacg uaugaccacu     1560

acagacaccu auuugcuggc ggaggaugug cucccaaagc agucucugau uuaccagcgg     1620

uuugagguac uuaaugaacu gaacgguuug aagaucgacg aucagcccau cacgaaugag     1680

cucaaacaag cuauuuucaa cgaccuguuu augcagaaga ccuccguguc agucaaaaau     1740

auacaggauu acuuagugag ugaaaagcga uaugccagca gaccggcaau cacagggcug     1800

uccgacgaga aaaaguucaa cucuaggcua agcacuuacc augaucucaa aguuauugug     1860

ggagacgccg ucgacgaugu ggacaagcag gcugaucugg aaaagugcau cgagugguca     1920

accauuuuug aggacggcaa aaucuauagu gccaaacuua augaaauaac augguugacu     1980

gagcaacagc gcggccagcu ggcaaccaaa cgguacagag gguggggaag gcucuccgcu     2040

aagcuguuaa cacaaaucgu aaacgccaau ggucagcgca uuauggaucu gcuuugggac     2100

accacugaua acuucaugcg aaucguucac uuugaagacu ucgauaaauu gauuacggag     2160

gcgaaucaga uggugcucgc cgaaaacgac guccaggaug ugaucaacga ccuguauaca     2220

agcccucaaa auaagaaggc ucuacggcag auacugcucg ucgugaacga uauucagaaa     2280

gcaaugaagg gccaggcccc cgagcguauc cugauugagu ucgcuagagg ggcccaaaaa     2340

gacaagucuc uuaccguuag gcgcaaacgg aaguuggacg aaauguacuc acagaucagu     2400

gaugcagugc uggcggacga gagcguaaga caggaauuaa aguccucuaa ucaggaugac     2460

cucgucaaag agaaggugua ucuguacuuu augcaaggag gcagggaucu uuacacuggu     2520

aacgcccuga auauagaccg auugucggau uaucacguug accauaucau uccacaggcu     2580

uacaucaaag augacagccu cgauaacaag gcccuggugu ccucacgcau gaaugaaauu     2640

aaagcagacc gggucccucu acagguggua cagaagcaau ucgggaagaa cauggcuagu     2700

gaguggggcu auaugcacag agccggacug aucucuagga aaaaguacga caaucucaug     2760

uuuaaccccg agaaucugaa caaauaucag auaaaucguu ucauuagccg ccagcuugug     2820

gaaacccaac agguuaucaa guuggucgca aaccuguuau ccaaucagua cgagggcgau     2880

aacgugaaaa ucauuacagu gaagucaaaa cucacccaga cuauccggag cgaauuugac     2940

uucccaaaga acagagaggu caauaacuau caucacgccu uugaugcuua ccugacagcg     3000

uucguuggga uguaccuucu gaagagguau cccaaacuau cuuccuacuu uacguaugga     3060

gaguaccuca aggguaauca acaggacaaa uggaguagcu ucaacuuucu gaaugauuug     3120

aagcgagcca aacuggaaga caagaccaac aaggagauuu ucuggcauaa agaaucuggc     3180

uuagcauauc ucaauaagau auaccaguuu aaaaagaucc uggugacucg ggaggugcac     3240

gaaaacuccg gggcucuuuu caaucagaca cuguaugccg ccaaagauga caaggcuuca     3300

ggacaaggcg guaagcaguu gauuccugca aaacaggccc gcccaaccgc gcucuacggg     3360

ggcuacagug gaaagacagu agcuuauaug uguaucguca gaauuaaaaa caagaaaggc     3420

gaucuguaca aggugugcgg gguugagacu agcuggcuag cccagcugaa gcaacuuaaa     3480

gacgaggauu cgcagaaggc auuccucaaa cagaagaucu ccccgcaauu uaccaaagcc     3540

aagaagcaga aaggaacgau agugaagguc guggaagacu ucgagguuau cgcuccccac     3600

auucugauca aucagagguu guuugauaac ggucaggaac ugaccuuagg cucugcaaca     3660

uauaaacaua augagcaaga acucauucug gacaagacug ccgugaaacu ucugaacggg     3720

gaguugccuc ucgcgcagag cgaggaacug gcugagcuag uauacgacga aauccuggau     3780

caggucaugc acuauuuccc acuuuacgac accaaucagu uucgggccaa gcuccuggca     3840

ggaaaaacag ccuucaugca auugcccugg aagucaaacc cugaugacaa caagacugug     3900

cagauaaccc agccaauugu guuaaagaag auccugcagg gccuccaugc ugaugccggu     3960

cgagucaguc ugaaagagcu ugggaugacg acagacuuug gcaugcugca aaaggcaacc     4020

ggaaucacuu ucacacccga uacccagauu auauaucagu ccccgacugg cuuguuugaa     4080

agacgcguug cucuaaggga ccuguga                                         4107


<210>  3106
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3106
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggaucugc      240

uaucugcagg agauuuucag caacgaaaug gcuaaaguag acgauuccuu uuuccaucgc      300

cuagaggaau cuuuucucgu ggaggaggac aagaaacacg aaagacaucc uaucuucgga      360

aauauagucg augagguggc cuaccacgaa aaguauccaa ccaucuacca ucugaggaag      420

aaacuuguug acucgacaga uaaggcagac cugcgguuga uuuaucucgc ccuggcucac      480

augaucaaau uucgcggcca cuuccugauu gagggugauc ucaacccgga caauucagac      540

guggauaagu uauuuaucca acugguccag accuacaacc agcuuuucga ggaaaauccc      600

auaaacgcaa gcggggugga cgccaaagcu auuuugagug cccgacuguc caagucuaga      660

aggcuagaga aucucaucgc gcagcugccu ggagaaaaga agaacggccu uuuuggcaau      720

cugauugcau ugagccucgg gcugacucca aacuucaagu ccaauuuuga uuuagccgag      780

gacgcuaaac ugcaacucuc aaaggauaca uacgacgaug accuugauaa ccuguuggcc      840

cagaucggag accaguaugc agaucuauuc cuggcugcca aaaaccucag ugacgcgauc      900

cugcuuagcg acauucugcg gguaaauacc gaaauaacga aggcccccuu gucugcaucc      960

augaucaaac guuacgauga gcaucaccag gaccucacuc uguuaaaggc ucugguucgc     1020

caacagcuac cugagaagua uaaagaaauu uucuuugauc agucaaagaa cgguuacgcc     1080

ggcuauaucg acgggggagc uagccaggag gaguucuaca aauuuauaaa gccaauucuc     1140

gagaaaaugg auggcaccga agaacugcuu gugaaguuga auagagagga ccugcucagg     1200

aagcaacgga cauucgauaa cgguaguauc ccccaucaga uccaccuggg ggaacuucac     1260

gccauucugc gacgccagga ggacuuuuau ccuuucuuga aagauaauag agaaaagauc     1320

gagaaaauuc ucacuuuuag gauaccauac uacgucggac cgcuggcacg gggcaacucu     1380

cgcuucgccu ggaugaccag aaaguccgaa gagacaauca cccccuggaa uuuugaggaa     1440

gugguggaca aaggcgcuag cgcgcaaucg uucauugaga ggaugacgaa cuuugauaag     1500

aauuuaccua acgaaaaggu ccugccaaaa cauucucuac ucuaugagua cuucacuguu     1560

uauaaugagc ugacaaaggu gaaauacgua accgaaggga ugcguaagcc cgcauuucuu     1620

uccggagagc agaaaaaggc caucguggac uugcuguuca aaacuaaccg gaaggucaca     1680

gugaagcagc ucaaagaaga uuauuucaag aaaaucgagu guuuugacuc aguugaaauu     1740

aguggugugg aggaccgauu caaugcuagc cugggcaccu accacgauuu acugaagaua     1800

aucaaagaca aggauuuucu ugacaacgag gaaaaugagg auauuuugga agacaucguc     1860

cucacgcuga cccuauucga ggauagagaa augauugagg agaggcugaa gacauaugcc     1920

caucucuuug acgauaaagu gaugaagcag cugaaacgcc ggagauacac ugggugggga     1980

aggcuuuccc gcaaguugau caacggcaua cgagacaaac aaucugguaa gaccauccug     2040

gauuucuuaa agagcgacgg guuugcaaac cggaauuuca ugcagcucau ucacgaugac     2100

ucacugacau uuaaagaaga cauccagaag gcccagguaa guggccaagg agauucccuu     2160

caugagcaca uugcuaaccu ggccggcagc ccugcaauca aaaaggggau auugcagacu     2220

guuaaagugg ucgacgaacu cgugaagguc augggacguc acaagccaga gaauauugug     2280

aucgaaaugg cgagagagaa ccagaccaca cagaaagguc aaaagaacuc uagggagcgc     2340

augaaacgga uugaagaggg caucaaggaa cuggggucac agauacuaaa agagcauccc     2400

guugagaaua cccagcugca gaaugaaaag cucuaccugu auuaccuuca aaacggaaga     2460

gauauguaug uggaccagga guuggauauc aauaggcuga gugacuacga uguagaccac     2520

auugucccgc agagcuucuu aaaagaugac uccaucgaua acaaggugcu cacucgaucu     2580

gacaagaauc gcggcaaauc ggacaacguu ccuagcgaag agguggucaa gaaaaugaag     2640

aauuauuggc ggcaacugcu uaacgcuaaa cugauuacgc agagaaaguu ugauaaucua     2700

acaaaggccg aaaggggugg gcucuccgag cuggacaaag caggcuucau caagcgucag     2760

uugguggaga cccgccagau aacuaaacau guagcccaaa uucuggauuc acggaugaac     2820

accaaguacg acgaaaacga uaaauuaauc agagagguga agguuaucac acucaagagu     2880

aaacuggucu cugacuuuag gaaggauuuc caguucuaua aagugcgaga aauuaauaac     2940

uaccaccacg cucaugacgc cuaccuuaau gcaguggucg gaaccgcucu gaucaagaaa     3000

uaucccaagu uggagagcga auuuguuuac ggcgauuaua agguguacga cgugcggaaa     3060

augauugcca aguccgagca ggagauaggg aaagcgacug ccaaguauuu cuuuuacuca     3120

aacaucauga auuucuuuaa aacagaaauu acgcucgcua acggagagau ccgcaagaga     3180

ccacugauug aaaccaaugg ugagacuggc gaaaucguau gggauaaggg gagggacuuc     3240

gcaacagucc ggaaagugcu aagcaugccu cagguuaaca uagugaagaa aaccgagguc     3300

caaacaggag gcuuuucuaa ggaguccauc cugcccaaac gaaauaguga uaaacuuauu     3360

gcccgcaaaa aggacuggga cccaaagaaa uauggugggu ucgauagccc cacuguggcu     3420

uacucuguuc ucgugguagc caaggucgaa aaaggcaagu ccaaaaagcu gaagucagug     3480

aaagaguugc ugggaaucac cauuauggaa agaaguagcu uugagaagaa cccuaucgac     3540

uucuuagagg caaaaggcua caaggaagug aaaaaggauc ucauaauuaa gcugccaaaa     3600

uauucgcuuu uugagcugga aaaugggagg aagcguaugu uggcguccgc cggagagcuc     3660

cagaaaggca acgaacuggc ucuaccgucu aaguacguca auuuccugua ucuugccagc     3720

cacuacgaga aacucaaggg uucacccgag gacaacgaac agaagcagcu guucguugag     3780

caacauaaac acuauuugga ugaaaucauc gagcagauua gugaauuuuc caagcgggug     3840

auauuagcag acgcuaaucu ggauaaagua cucucugccu acaacaagca uagagacaaa     3900

ccuaucaggg agcaggcaga gaacauuauc caccuguuca cgcuuaccaa ucugggggcc     3960

ccagcugcgu uuaaguauuu cgauacaacu auugaccgca aacgauacac cagcacaaag     4020

gaaguguugg augccacucu aauccaccaa uccauaaccg gacuguacga gacgagaauc     4080

gaccucucac agcugggcgg ugauuga                                         4107


<210>  3107
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3107
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aagcucaaag gucuuggcaa uacugacaga      120

cacgggauca agaagaaccu gauuggagcu uuguuauucg auucaggcga gacggcagaa      180

gccaccaggc ugaaacggac agcgcgccga cguuacacua gaaggaagaa ucggaucugc      240

uaucuacagg agauuuuuag uaacgaaaug gcuaaagugg acgauagcuu cuuucaucgc      300

cucgaggaau ccuuccuggu agaggaggac aagaaacacg aaagacaucc uaucuuuggu      360

aauauagugg augaggucgc cuaccacgaa aaguauccaa ccaucuacca ucuuaggaag      420

aaacuggcag acucuacaga uaagguggac uugcggcuca uuuaucuggc ccuggcucac      480

augaucaaau uccgcgggca cuuucucauu gagggagauu uaaacccgga caauucggac      540

guugauaagc uguucaucca acuugugcag accuacaacc aguuguuuga ggaaaauccc      600

auaaacgcau cacgagucga cgccaaagcu auucugagcg ccagacuaag uaaguccagg      660

cggcucgaga aucugaucgc gcagcuuccu ggcgaaaaga agaacggccu guucgggaau      720

uugauugcac ucucucuggg auuaacucca aacuuuaaga gcaauuucga ucuggccgag      780

gacgcuaaac uccaacuuuc caaggauaca uacgacgaug accuggauaa cuugcuagcc      840

cagaucggug accaguaugc agaucuguuu cucgcugcca aaaaccuguc agacgcgacc      900

cuucugagug acaucuugcg ugugaauagc gaaauuacga aggcaccccu cucugccucc      960

augauaaaac gcuacgauga gcaucaccag gaccugacuu uacugaaggc ucuaguaaga     1020

caacagcucc cugagaagua uaaagaaauc uucuucgauc agucaaagaa cggcuacgcc     1080

ggguauauug acggaggcgc uagccaggag gaauuuuaca aauucaucaa gccaauacug     1140

gagaaaaugg augguaccga ggaacuuuug gccaagcuga auagggagga ccuccugcgg     1200

aagcaacgaa cauuugauaa cgggaguauu cccuaucaga uccaucuugg agaacugcac     1260

gcaaucuugc gcagacagga ggacuucuac ccuuuucuca aagauaauag ggaaaagauu     1320

gagaaaaucc ugacuuuccg gauuccauac uauguuggcc cguuagcccg cggcaacucu     1380

agauuugcuu ggaugaccag gaaguccgaa gagacaauaa cccccuggaa uuucgaggaa     1440

guggucgaca aaggggcgag cgcacaaucg uuuaucgagc guaugacgaa cuucgauaag     1500

aaucugccua acgaaaaggu gcuaccaaaa cacucucucc uguacgagua uuuuacugug     1560

uacaaugagc uuacaaaggu caaauauguu accgaaggaa ugcggaagcc cgccuucuug     1620

uccggugagc agaaaaaggc uauuguggac cugcucuuua aaacuaaccg aaagguaaca     1680

gugaagcagc ugaaagaaga uuacuucaag aaaaucgagu guuucgacuc agucgaaauc     1740

aguggcgugg aggacagauu uaaugccagc uuagggaccu aucaugaucu gcuuaagauu     1800

auaaaagaca aggauuucuu ggacaacgag gaaaaugagg auauccucga agacauuguu     1860

cugacgcuaa cccuguuuga ggauagggaa augaucgagg agcgccucaa gacauacgca     1920

caccuguucg acgauaaagu gaugaagcag cuuaaacgga gaagguacac uggauggggc     1980

cgcuuguccc gaaagcugau uaacgguauc cgggacaaac aaucugggaa gaccauauua     2040

gauuuucuca agagcgacgg cuucgccaac cguaauuuua ugcagcugau ccaugaugac     2100

ucacuuacau ucaaagaaga cauucagaag gcucagguca guggacaagg cgauucccug     2160

cacgagcaua ucgccaacuu ggcagggagc ccugcgauua aaaagggaau ccuccagacu     2220

gugaaaguag uugacgaacu ggugaagguc auggguagac acaagccaga gaauauagug     2280

auugaaaugg cuagggagaa ccagaccaca cagaaaggcc aaaagaacuc ucgcgagcgg     2340

augaaaagaa ucgaagaggg gauuaaggaa cuaggaucag auauccugaa agaguauccc     2400

gucgagaaua cccagcucca gaaugaaaag cuguaccuuu auuacuugca gaacggcagg     2460

gacauguaug uggaucaaga gcuggacaua aaucgauuaa gugauuacga cguugaucac     2520

aucgugccgc agagcuuucu caaagacgau uccauugaca acaagguacu gacucgcucu     2580

gacaagaauc gggguaaauc ggauaacguc ccuagcgaag aggugguuaa gaaaaugaag     2640

aauuauugga gacagcuucu gaacgccaaa cuaaucacgc aaaggaaguu cgacaaucuc     2700

acaaaggcag aacguggggg ccuguccgag uuggauaaag ugggauuuau uaagcgccag     2760

cuggucgaga cccggcagau cacuaaacau guggcccaga uauuagacuc aagaaugaac     2820

accaaguacg augaaaacga caaacucauu agggagguac gagugaucac acugaagagu     2880

aaacuuguuu cugauuuccg gaaagacuuu caauucuaca agguccgcga aaucaauaac     2940

uaucaccaug cucacgaugc cuaccugaau gcaguggugg gcaccgcuuu gauuaaaaag     3000

uaucccaaac ucgagagcga auuugucuac ggggacuaua agguuuacga ugugagaaag     3060

augaucgcca aauccgagca ggagauugga aaggcgacug ccaaauauuu cuucuacuca     3120

aacauaauga acuuuuucaa gacagaaauc acgcuggcua auggugagau uaggaaacgg     3180

ccacuaaucg aaaccaaugg cgagacuggg gaaauugugu gggacaaggg acgagauuuu     3240

gcaacaguaa gaaagguccu gagcaugccu caggugaaca ucguuaaaaa gaccgaggug     3300

cagacaggcg guuucucuaa agaguccaua cuucccaagc gcaauaguga caaacucauc     3360

gccaggaaga aagacuggga uccaaagaag uacgggggcu uugacagccc cacugucgcu     3420

uauucugugc ugguuguggc caaaguagaa aagggaaaau ccaagaaauu gaagucaguc     3480

aaggagcugu uaggcauuac caucauggaa cguaguagcu ucgagaaaga uccuauugac     3540

uuucucgagg caaaggggua caaagaagug cggaaggauc ugaucauaaa acuuccaaag     3600

uauucgcugu ucgaguugga aaacggaaga aagaggaugc ucgcguccgc cggcgagcug     3660

caaaaaggua augaacuagc ucugccgucu aaguacguga acuuucuuua ucucgccagc     3720

cacuacgaga aacugaaggg gucacccgag gacaaugaac agaaacaguu guucgucgag     3780

cagcauaagc acuauuuaga ugaaauuauc gagcaaauca gugaguucuc caagcgcguu     3840

auucuggcag acgcuaaccu cgauaaagug cugucugccu acaauaagca ucgagacaaa     3900

ccuauaagag aacaggcaga gaacaucauu caccuuuuua cgcugaccaa cuugggagcc     3960

ccagcugcgu ucaaguacuu ugauacaacu aucgaccgga aaagguauac cagcacaaag     4020

gaaguacuag augccacucu gauucaucag uccaucaccg gccucuacga gacgcgcaua     4080

gaccugucac aacuuggugg cgacuga                                         4107


<210>  3108
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3108
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggaucugc      240

uaucugcagg agauuuucag caacgaaaug gcuaaaguag acgauuccuu uuuccaucgc      300

cuagaggaau cuuuucucgu ggaggaggac aagaaacacg aaagacaucc uaucuucgga      360

aauauagucg augagguggc cuaccacgaa aaguauccaa ccaucuacca ucugaggaag      420

aaacuugcag acucgacaga uaaggccgac cugcgguuga uuuaucucgc ucuggcacac      480

augaucaaau uucgcggcca cuuccugauu gagggugauc ucaacccgga caauucagac      540

guugauaagu uauuuaucca acuggugcag accuacaacc agcuuuucga ggaaaauccc      600

auaaacgcca gccgagucga cgcuaaagcc auuuugagug cgagacuguc caagucuagg      660

cggcuagaga aucucaucgc acagcugccu ggggaaaaac guaacggacu uuuuggcaau      720

cugauugccu ugagccucgg ccugacucca aacuucaagu ccaauuuuga uuuagcugag      780

gacgccaagc ugcaacucuc aaaagauaca uacgacgaug accuugauaa ccuguuggca      840

cagaucgggg accaguaugc ugaucuauuc cuggccgcga agaaccucag ugacgccauc      900

cugcuuagcg acauucugcg cguaaauucu gaaauaacca aagcacccuu guccgcuuca      960

augaucaaga gauacgauga gcaucaccag gaccucacgc uguuaaaagc ccuggugagg     1020

caacagcuac cugagaagua uaaggaaauu uucuuugauc agagcaaaaa cggauacgcu     1080

gguuauaucg acggcggggc cagucaggag gaguucuaca aguuuauaaa accaauucuc     1140

gaaaagaugg auggaacuga ggaacugcuu gcaaaauuga aucgggagga ccugcuccga     1200

aagcaacgca ccuucgauaa cggcucuauc ccccaucaga uccaccuggg ugaacuucac     1260

gccauucuga gaaggcagga ggacuuuuau ccuuucuuga aggauaaucg ggaaaagauc     1320

gagaagauuc ucacauuucg cauaccauac uacguugggc cgcuggcuag aggaaacucc     1380

agguucgcgu ggaugacucg uaaaagcgaa gagaccauca cacccuggaa uuuugaggaa     1440

guggucgaca agggcgcauc ggcccaaucu uucauugagc ggaugaccaa cuuugauaaa     1500

aauuuaccua acgaaaaggu gcugccaaag cauucccuac ucuaugagua cuucacggug     1560

uauaaugagc ugacuaaagu caaguacguu acagaaggca ugcgaaaacc cgaguuucuu     1620

ucaggggaac agaagaaagc uaucguggac uugcuguuca agaccaacag aaaaguaacu     1680

gugaagcagc ucaaggagga uuauuucaaa aagaucgaau guuuugacag ugucgagauu     1740

agcggagugg aggacagguu caaugccucc cuggguacau accacgauuu acugaaaaua     1800

aucaaggaca aagauuuucu ugacaacgaa gagaaugaag auauuuugga ggacaucguu     1860

cucacccuga cgcuauucga agaucgcgag augauugagg aacggcugaa gaccuaugca     1920

caucucuuug acgauaaggu gaugaaacag cugaagagaa ggcgcuacac aggcuggggg     1980

cgacuuucuc ggaaauugau caacggaaua cgugacaagc aaagcggcaa aacuauccug     2040

gauuucuuaa agucagacgg uuuugccaac agaaauuuca ugcagcucau ucacgaugac     2100

agucugaccu uuaaggagga cauccagaaa gcucaggucu ccgggcaagg cgauagccuu     2160

caugaacaca uugccaaccu ggcaggaucu ccugcgauca agaaaggcau auugcagaca     2220

gugaagguag uugacgagcu cgugaaaguc auggggaggc acaagccaga aaauauugug     2280

aucgagaugg cucgcgagaa ccagacuacc cagaagggac agaaaaauuc acgggaaaga     2340

augaagagga uugaggaagg uaucaaagag cugggcagug auauacuaaa ggaguacccc     2400

gucgaaaaca cacagcugca gaaugagaaa cucuaucugu acuaucuuca gaacgggcga     2460

gacauguacg uggaucaaga auuggacauc aaucgccuga gcgauuauga cguugaucau     2520

auugugccgc aguccuucau caaggacgau ucuauugaca acaaaguauu aacccggucg     2580

gacaagaaua gaggaaagag cgauaacguc ccuuccgagg aagugguuaa aaagaugaaa     2640

aauuacugga agcagcuccu gaacgccaaa cuuaucacuc aaaggaaguu ugacaaucug     2700

acgaaggcag agcguggcgg ucuaucagag cucgauaaag ccggguucau aaagcgccag     2760

cugguggaaa cacggcagau uaccaaacac gucgcucaga ucuuggacag uagaaugaac     2820

acuaaguaug augagaacga caaacugauc agggaaguga agguaauuac cuuaaagucu     2880

aaacucguga gcgauuuucg aaaggacuuc caauucuaca aaguucggga gaucaauaac     2940

uaccaucacg cccacgaugc auaucugaau gcugucgugg gcacagcccu uauuaagaaa     3000

uaccccaagc uggaauccga guuuguguau ggagacuaca aggucuauga uguucgcaaa     3060

augauagcga agucagagca ggaaaucggc aaagccaccg cuaaguacuu cuuuuauagc     3120

aacauuauga auuucuuuaa aacugagauc acauuggcaa acggggaaau uagaaagagg     3180

ccacucaucg agacgaaugg agaaaccggu gagauagugu gggacaaggg ccgggauuuc     3240

gccacugugc gaaaaguacu gucuaugccu caggucaaca ucgugaagaa aacagagguu     3300

cagaccgggg gauuuuccaa ggaaaguauu cuacccaaaa gaaauagcga caagcugauc     3360

gcucgcaaaa aggacuggga uccaaagaaa uacggcgguu ucgacucucc cacaguggcc     3420

uacuccgucc uugugguugc aaagguggag aaagggaaga guaaaaagcu caagucagua     3480

aaagaacugu ugggcauuac uaucauggag aggagcucgu uugagaagaa cccuauagau     3540

uuccuggaag cgaaaggaua uaaggagguc cguaaagacu uaauuaucaa gcucccaaag     3600

uacucccugu uugaacuuga gaauggccgg aaaagaaugc uggccucugc uggggaauug     3660

caaaagggaa acgagcucgc ccugccgagc aaauauguga auuuccuaua ccuggcauca     3720

cauuaugaga aacuuaaagg cagucccgaa gauaacgagc agaagcaacu cuucguggaa     3780

cagcacaagc auuaccugga cgagaucauu gaacaaauau ccgaguuuuc uaaaaggguc     3840

aucuuggcug augccaauuu agacaagguu cugagcgcau auaacaaaca ccgcgauaag     3900

ccuauucgag agcaggccga aaacaucauu caucucuuca cccugacgaa uuuuggugcu     3960

ccagcggccu ucaucuacuu ugacaccaca auagggagaa accgguacaa auccaucaag     4020

gaggugcuug augcaacucu gauucaccag ucaaucaccg gauuguauga aacaaggauu     4080

gaccuaagcc aacugggcgg ugauuga                                         4107


<210>  3109
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3109
augaaguacg agagcgaaga guauuacauc ggccuggaca uggggaccuc cucuguggga       60

ugggccguca cagaucccca cuaucaucuc auuagaaaga aggguaaaga ccuuuggggc      120

gcuaggcugu ucgaaaaggc aaacacugcc caggagcggc gcacgaaucg aauaaaccgu      180

agaaggaauc aacggcgcaa acagagaauc gcguuuauua gggaggcuuu ccggaaggag      240

guugagaagg uggauguaaa cuuuuuccgc cgauuggacg aaucacaguu auaccuggag      300

gauaaaagua ucaaggaacc uuaucuacuc uuuaaugacc ugaacuacac cgauaaagac      360

uucuaucacc aguacccaac aguguuucau cuuauuacug aucuguugac cagcgacaga      420

aaguucgaua ucagggagau auacaucgcc gucuccucuc ucuauaauca ccgggggaac      480

uuucugaaua aaucgcuggg aaacgagaca caagacgaag aucucguguu caagaauuua      540

gacgaggcau uuucagccug cggcaugaac uggaauacca aggcugaaaa cauugagguu      600

gugaaacaga uccuguguga ccguagccuu aauaagaaag aaaagcagga ucaguuggca      660

cauugccugg agauuaacaa aaagacugag aaagaaaaga ucgaguuccu aaagcuccug      720

acagguggga aagccgacag uaagaaaaug uuuccgcaau accacuauga acgcgaugag      780

aaacuuuccu ucgcuuucgc cgacgaaaac ggagaugaga auugggagga aacccugaac      840

gcgauagagg aaagauugac gucugacgag uacggccuca uucuggcauu aaaaagcguc      900

uaugauuggu ccauccuggc cgaccucaug aagaaugaaa agacuggcga gaccuacccc      960

uaucuuucaa uggcuaggaa agauaguuac gagaagcaca aagccgaccu gcgguugcua     1020

aagagcguga uuaaacgacu gaucccuaac gaauacaauc auuucuuccg ccaguaugau     1080

gggaagggaa agacauacac uaacuauauc gguucuguac aguaccacga gaaaagacau     1140

agguuuaagc acauuaccac aaaagaauuu uaugacggcc ucaagaaacu gcuuaagcca     1200

uacuccgagg acgaggaaau aaagcagauc cugcaagaua uugagucaga aaccuuuuug     1260

cccaaacaga ucacgaauau gaacgggguu auaccuaauc aggugcacgc aaaggagcuc     1320

aaagucauuc uggaaaaggc uagccaacau uaugacuucu uaaacgagac uaaugagagu     1380

ggacugacag ugaaagaaca gaucaugcag auguuuaccu uccagauccc auacuacgug     1440

ggcccccuag ccaaccucaa ugguaagggg aacggauggg cgguccggua uguuggcaaa     1500

gagcgcacua agguguaccc uuggaauaug gaugaaguaa uugacgagaa ggaaacagcc     1560

gaucaauuua ucaaaagaau gauuucuacc uguacguauc ugggcgacga guacgugcuu     1620

ccaaagucca gcauauugua ugagaaauuc gcaguccuga acgaacucaa cucgcugaag     1680

aucaaugggc agccgguguc uguugaucuu aaacagaaga uuuacaggga ccuguuuacc     1740

aagggaaaac gugugacaca gaaggguuug cuccuguauu uaaaacggga gggcgucauc     1800

uccaagucag aagagacuau ccugaguggg uucgauauug acuuuaaaaa cacccuaagc     1860

uccuaccgaa aguucugcga aguguucgcu acaaaugagc ucaacgauga gcaaauaaga     1920

auggccgaag acaucauuga guggcugacu guauuuggag auucuaagaa auucguuagg     1980

gaaaagaucc acggcaauua cccccagauu accgcuaacc agcuuaaacg caucuugagc     2040

uuuaauuuca aggacugggg uaaacuguca aagcaguuuc ucgagcugga gggggaagac     2100

cgggagacag gcaagaguug uuccuuaaua caagcccugu gggauaccaa caauaaccuu     2160

aaucaguugc ucagcucuag auucacuuau aaagaaaacc uggagaagaa aaggaagaaa     2220

aucgaaaugg accuauuuga guucacgcau gaggaucugg acgaucucua cauuucagca     2280

ccugugaagc gcaugaucug gcaggcccug aaaauuauga aggacaucgu cauagugaug     2340

aaggaaaguc caacaaaacu uuuuauugag augacccgaa auaacgaaaa ugagaagacu     2400

cggaccguca gccguaaaaa caaguuggug gaucuguaua aaaagaucaa gggagaaucc     2460

agagacugga uuaaagaguu agagagguac gaagagaagg auuuccgcuc uaaaaagcuc     2520

uaucuguacu auacacagau gggcaaaucg gcuuacagcg gggaacccau cgacauggau     2580

aaacuuuuug acaagaacgu uuacgauaua gaccacaucu auccuaaguc caaggugaaa     2640

gacgauucac uguugaauaa cugcguacuc gucaagcgga aagagaauau ggacaaggga     2700

aacaauuucc caauugaucc cgacauccaa accaagcagg aacaucugug gaaauuuugg     2760

cuagagaagg gucugauuac ucaggagaaa uucaacagac ucacaaggaa gaaaccguuc     2820

acgaaugaug aacuggcgga cuuugugaac cgacaacuug uugagaccgg ccaggcaacu     2880

aagacaaucg ccgauuuguu caagaugacc uuuccuaaua gugacauagu guacgucaaa     2940

gcugggaacg ugacagauuu ccgccaguau auugaaguau acaagugucg ggaggugaau     3000

aaccugcacc acgccuauga cgcauacuua aauguugucg ugggaaacgu guaugauacu     3060

aaauuuacca agaaucccau gaacuacauc aaagagcaga gaaaggaaaa cucuaaugag     3120

agguaccaua uggacaaaau cuucgaauuu ccagucauuc guaacggcaa ggaggccugg     3180

aucacgaaga aagacgaaag cauuaauaua guucgcaaga cccucgcucg gaaaacaccu     3240

cuggugacua agagaaacuu cgaggccaau gguaccaucg caaaagcgac aauuuauccc     3300

aaggggaacg ugaaggauca aggcuaccuu ccaaggaaaa cuuccauccc cgcucugcga     3360

gacaugaaga aauauggagg cguaaccuca auucagaucg ccuacuuuuu ccuagucgag     3420

cacgaaguga agaaaaagcg gauacgcacg aucgagugcg uuccuauuua uagaaaggaa     3480

gaacucaaaa caaaggaaca gcuggagagg uacugucggg aggaauugaa acugaucaau     3540

ccaagcauuc gauuagagaa gaucaaaccg uauucucucc ugaagaugaa cgggaacuuu     3600

uucuacauaa ccggaaagac uaaugauaga uucucccuug caaaugccau ggaacuguac     3660

guggacaaag ucagugagaa ccauauucgc gauuugguga aguauaaaaa uugcgguuac     3720

uuuaacguug acgauaagga agcuaggaaa cguaaugagc ggcucuauac aauccugacc     3780

aagaaacacc uaacuguguu caagggcagc cccaagccua ugggggagaa acugcagcaa     3840

uaccagcagc uuuuucucga acuggaggua ucuaagcagg ucuauguguu gcugcaaauc     3900

uuacagcuca ccgccacaaa cgugaaaacg aaggcagacc ugaccgcuau uggaggcaaa     3960

gccaaugaag guacuaugga uauauccaag aaccuugaga acgucaucaa gaaucagauu     4020

aaaaaggggc ugguuccaga guugaugcuc aucaaccaau cacccacagg ccuguucgaa     4080

agugagauug accuacugac caucuga                                         4107


<210>  3110
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3110
augagcagac ccuacaacau cggccuggac auugggaccu ccucuauagg auggucagug       60

gucgaugacc agaguaagcu cguuagcgug agggguaaau auggcuacgg gguacggcuu      120

uaugaugagg gacaaacagc cgcugaacgc cgauccuucc guacuacgag aaggcggcug      180

aagcgcagaa aauggagguu gggcuuacug cgggagaucu uugaaccuua cauuacccca      240

guggacgaua cauucuuucu acgcaagaaa cagucuaauc ucucgccgaa ggaccagcga      300

aagcuguauc cccagacuuc acuuuucaac gauagaaccg acagggcauu uuacgaugac      360

uauccuacaa ucuaccaucu gcgguacaaa uugaugaccg agaagcguca auucgauauu      420

cgcgaaaucu aucucgccau gcaccacaua gucaaauaca gaggucauuu ucugaaugag      480

gcgccaguga gcaguuucaa guccucugag aucaaccugg uugcucacuu ugacaggcuc      540

aauacuauuu ucgccgauuu auuuagcgaa uccggguucc agcuggagac agacaaacuu      600

gcagaaguga aggccuugcu gcuagacaac caucagucag cuaguaaucg gcagcgacaa      660

gcacuccugc uuaucuauac ccccagcacg aacaaggccg ucgagaaaca gaauaaggcu      720

auugccacug agcuguugaa agcgauccuc ggacugaagg caaaauuuaa cguguuaacc      780

ggcauagaag ccgaggaugu aaagacaugg acucugaccu ucaaugcuga aaacuucgac      840

gaggaaaugg uuaaacucga gucuucccuu gaugacaaug cccaccagau uaucgaguca      900

cugcaggaau uguacagcgg cgugcuacug gcagggauug ucccugagaa ccaaagucuc      960

ucucaggcua ugaucacaaa guaugaugac caccagaagc aucugaaaau gcuuaaggcc     1020

gugcgcgaag cgcuggcccc agaggauaga caaagguuga aacaggcaua cgaccaguau     1080

guggauggac aggaaaauac caaggcuuac uccaaagagg acuuuuacgg ugauaucacg     1140

aaggcccuca agaacaaccc cgaccacccu auugucagcg agauaaagaa gcugaucgaa     1200

uuagaccaau ucaugccaaa acagcggacu aaggauaaug gcgcuauucc gcaucagcug     1260

caccagcaag agcuagaccg caucauagaa aaccagcagc aguauuaccc cuggcucgcc     1320

gagcugaauc cuaacucgaa aagacaaaca guugcaaagu auaaacuuga ugaauuggug     1380

gccuuuaggg uaccauacua uguggggccc cugauuaccg cugagcagca gcagcaaucu     1440

uccgacgcga aauucgcaug gaugauccgu aaggccgagg gacagaucac uccuuggaau     1500

uuugaugaca aagucgaucg gcaggcuuca gccaacgagu ucauuaagcg aaugacaacc     1560

acggacaccu accuccuggc agaggaugug cuuccaaaac aaagucugau cuaucagaga     1620

uuugaaguuu ugaaugagcu caacggccug aagauugacg aucagcccau aacaacugag     1680

uuaaagcagg ccaucuucac cgaccuguuu augcagaaaa caagcgugac ugucaagaau     1740

auucaggauu accuaguguc cgaaaagagg uacgcuucuc gcccggccau caccggucuc     1800

agcgacgaga acaaguucaa uucacggcug aguacauauc acgaccuuaa aaccaucgua     1860

ggggaugcag uugacgaugu ggacaagcag gcggauuugg aaaaaugcau ugaguggucc     1920

acuauauuug aagacggaaa gaucuacagc gcuaagcuga acgagauuga uuggcucacg     1980

gaccagcaaa gaguccagcu ggccgcaaaa agguaucgcg gcuggggccg auuaucugcc     2040

aagcugcuua cacagaucgu gaaugcuaac gggcagcgga uuauggauuu gcucugggac     2100

accacugaua auuucaugcg uaucguccau ucagaggacu uugauaaacu gauaaccgaa     2160

gccaaccaaa ugaugcuagc agagaacgac gugcaggacg uuaucaauga ucuguacaca     2220

aguccucaga acaagaaagc ucucagacaa auucugcuug ugguaaauga cauccagaag     2280

gccaugaagg gacaggcgcc cgaaaggauu uugaucgagu ucgcccgcga agaugagguc     2340

aacccacggc ugagcgugca gagaaaaagg caaguugaac agguguauca gaauauaucc     2400

aacgaauuac ucaauaacac cgagauucga aaugaacuga aggaccuuuc uaacucggcu     2460

cugagcaaua cucgcuuguu ccucuacuuu augcagggug gccgggauau guauacaggg     2520

gacucccuga acaucgauag acuaucaacg uacgacauug aucacauccu gccucaaagu     2580

uucauaaaag acaauucucu cgauaacagg guccugguga gccagcguau gaaucgcucc     2640

aaggcagacc agguacccac cgacuuuacu ucaguggagc uuggacagaa aaugcaaauc     2700

cagugggaac agauguugcg ggccggccug auuacaaaga agaaauacga uaacuuaacc     2760

cucaacccag accauaucag caaguaugcu augaaagguu ucauuaauag acagcugguu     2820

gagacaaggc aagucaucaa acuugccacu aaccugcuaa uggagcagua cggggaagau     2880

aauauagagc ucauuaccgu gaaaucuggc cugacgcacc agaugcgaac cgaauuugac     2940

uuccccaaga accggaauuu gaacaaucau caccacgcau uugaugcgua ucugacagcc     3000

uucgugggau uauaccuccu gaagcgcuau ccuaaacuua agccauacuu ugucuauggc     3060

gaguaccaaa aggcuuccca gcaggacaag uggagaaacu ucaauuuucu gaacggguug     3120

aaaaaggaug agcucguuga cgaaaauacu gaggccguga ucuggaacaa agaaagugga     3180

cuggcauacc uaaauaagau cuaucaguuc aagaaaauuc uggugaccag ggagguacau     3240

gaaaacagcg gugcucuuuu uaaucaaaca cucuacgccg caaaggauga caaagccucu     3300

ggccaagggg gaaagcagcu gaucccggcu aaacaggauc ggcccacugc guuguauggc     3360

gguuacuccg ggaagaccgu cgccuauaug uguauugugc gaauaaagaa caaaaagggc     3420

gaccuguaca aaguuugcgg aguggagacg ucaugguuag cacaacucaa gcagcugaca     3480

gaugaggaca guaaaaaggc uuuccuuaag cagaaaauca gcccucaguu caccaagguc     3540

aaaaagcaaa aaggcacuau ugugaagguu guggaagacu uugagguaau cgccccacac     3600

auucugauca accagagauu cuuugauaau gggcaggaau ugacacucgg aucggccacc     3660

uauaagcaua acgagcaaga acugauacua gacaaaacug cugucaagcu gcuuaauggu     3720

gcacuccccc ugacccaguc cgaggaguug gccgaacagg uguacgauga gaucuuagac     3780

caggugaugc acuacuuccc ucuguaugau acaaaccaau uucgcgcgaa acucucugcu     3840

ggcaaggccg cauucaugaa acugccaugg aaaagccagu gggacgggaa uaagaugguc     3900

cagguuggac agcaagugau ucuugauagg guacugaucg gcuugcacgc caacgcugca     3960

gugucagacc uagguguccu gaaaauuagu acgccccucg ggaagaugca gcagccgucc     4020

ggcaucucuc ugagcccuga uacccagaua auuuaccaau ccccaacugg acuuuuugaa     4080

cgucgggugg ccuuaagaga ccuguga                                         4107


<210>  3111
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3111
augagcagac ccuacaacau cggccuggac auugggaccu ccucuauagg auggucagug       60

gucgaugacc agaguaagcu cguuagcgug agggguaaau auggcuacgg gguacggcuu      120

uaugaugagg gacaaacagc cgcugaacgc cgauccuucc guacuacgag aaggcggcug      180

aagcgcagaa aauggagguu gggcuuacug cgggagaucu uugaaccuua cauuacccca      240

guggacgaua cauucuuucu acgccagaag cagucuaauc ucucgccgaa agaccagcga      300

aagcuguauc cccaaacuuc acuuuucaac gauagaaccg acagggcauu uuacgaugac      360

uauccuacaa ucuaccaucu gcgguacaag uugaugaccg agaaacguca guucgauauu      420

cgcgaaaucu aucucgccau gcaccacaua gucaaguaca gaggucauuu ucugaaugag      480

gcgccaguga gcaguuucaa auccucugag aucaaccugg uugcucacuu ugacaggcuc      540

aauacuauuu ucgccgauuu auuuagcgaa uccggguucc agcuggagac agacaaacuu      600

gcagaaguga aagccuugcu gcuagacaac cagcaaucag cuaguaaucg gcagcgacag      660

gcacucagcc ugaucuauac ccccucuacg aacaaggccg ucgagaagca gaauaaagcu      720

auugccacug agcuucugaa ggcgaucuug ggacucaaag caaaguuuaa cgugcugacc      780

cgcauagaag ccgaggaugu aaaggcuugg acauuaacuu ucaaugccga aaacuucgac      840

gaggaaaugg uuaagcugga guccucacuc gaugacaaug cacaucaaau uaucgagagc      900

cuucaggaac uguacagugg cauuuugcua gcuggcaucg ugccugagaa ccagucucug      960

ucccaagcca ugaucaugaa auaugaugac caccagaagc accucaagau gcugaaagcg     1020

gucagagaag cccuugcacc agaggauagg cagcggcuga agcaggcuua cgaccaauau     1080

guggaugggc aggaaaauac caaagccuac agcaaggagg acuuuuacgg agauauuaca     1140

aaggcuuuga agaacaaccc cgaccauccu auagugucgg agaucaagaa acucauugaa     1200

cuggaccagu ucaugccaaa gcagcgcacc aaagauaaug gugccauccc gcaccaauua     1260

caucagcagg agcuggacag aauaauugaa aaccagcaac aguauuaccc cuggcuagca     1320

gagcucaauc cuaacucuaa gaggcagacg gucgccaaau auaagcugga ugaacuuguu     1380

gcuuuucgug ugccauacua uguaggcccc uugaucacug cggagcagca acggcagucc     1440

ucagacgcaa aguucgccug gcugauccga aaagcugagg ggagaauuac accuuggaau     1500

uuugaugaca agguggauag gcaggccagu gcaaacgaau uuaucaaacg caugaccacu     1560

acagacaccu accuccuggc cgaggauguc cuuccaaagc aaagccugau uuaucagcgg     1620

uuugaagugu ugaaugagcu caacggacug aaaauagacg aucagcccau cacgaccgag     1680

uuaaagcagg cuauuuucac agaccuguuu augcaaaaga cuuccguuuc ugugaaaaau     1740

auccaggauu accuagucag cgaaaagaga uacgccucaa ggccggcaau caccggccuc     1800

agugacgaga acaaauucaa uucccgccug agcacauauc acgaccuuaa ggcgauugug     1860

ggugaugcug uagaagacgu ugauaaacag guggacuugg agaagugcgu cgaauggucu     1920

acuauauuug aggaugggaa aaucuacuca gccaagcuga acgagauuga cuggcucacc     1980

gaucagcaac gagugcagcu ggcagccaag cgguaucgug gauggggcag auuaagugcu     2040

aaacugcuua cacagaucgu caaugccaac ggccagagga uuauggacuu gcucugggau     2100

accacugaca auuucaugcg caucgugcac agcgaagauu uugacaagcu gauaacggag     2160

gcaaaccaaa ugaugcuagc ugaaaacgau guucaggacg ugaucaauga ccuguacaca     2220

uccccucaga acaaaaaggc ccuccggcaa auucugcuug uagucaauga uauccagaaa     2280

gcgaugaagg ggcaggcccc cgagagaauu uugaucgagu ucgcuaggga ggacgaggug     2340

aacccacgac ugucuguuca gcgcaagcgg caaguggaac aggucuauca gaauauaucg     2400

aacgaguuac ucaauaacac cgaaauuaga aaugagcuga aagaucuuag caacuccgca     2460

cugucaaaua cuagguuguu ccucuacuuu augcagggag gucgugacau guauaccggc     2520

gauagucuga acaucgaccg ccuaucuaca uacgauauug accauauccu gccucaaucc     2580

uucauaaagg auaauagccu cgacaaccgg gugcugguau cacagaaaau gaauagaagc     2640

aaggccgauc aggugcccac cgacuuuacu ucuguugaac uugggaaaaa gaugcaguug     2700

caaugggagc agaugcugag ggcuggauua aucacaaaga aaaaguacga caaccucacg     2760

cugaacccag aucacauuuc caaauaugcc augaagggcu ucaucaaucg acagcuuguc     2820

gagacccgcc aggugauuaa acuggcaacu aaccuacuca uggaacaaua cggugaggac     2880

aauaucgaac ugauaacagu gaagaguggg uugacccauc agaugcggac agaguuugau     2940

uuccccaaga acagaaaucu gaacaaucac caucacgcgu uugacgccua uuuaacugcu     3000

uucgucggcc ucuaccugcu uaaaagguau ccuaagcuga aaccauacuu uguuuaugga     3060

gaguaccaga aggccagcca acaggauaaa uggcggaacu ucaauuucuu gaacggccuc     3120

aagaaagacg aacuggugga ugagaauacc gaagcaguga uuugggacaa ggagucuggg     3180

cuagcuuacc ugaacaagau cuaucaguuc aaaaagaucc uuguaacgcg agaaguccac     3240

gaaaauuccg gagcccucuu uaaccagacc cuguacgcag ccaaagauga caaggcuuca     3300

ggucaaggcg ggaaacaguu gauuccggcg aagcaggaua gacccacagc ccuguaugga     3360

ggcuacagug guaagacugu ggcauauaug uguaucguuc gcauuaaaaa uaagaaaggg     3420

gacuuauaca aggugugcgg cgucgagacc agcuggcucg cucagcugaa acaacuuaca     3480

gaugaagacu cgcagaaggc cuuccugaag cagaaaauau ccccucaguu cacuaaggug     3540

aaaaagcaaa aaggaaccau cguuaaggug guagaggacu uuaaggucau ugccccacau     3600

aucuugauua accagagguu cuuugauaac ggccaggaac ucacgcuggg gucugcuaca     3660

uauaaacaca augagcaaga acuaauccug gacaagaccg cagugaaacu ucucaacgga     3720

gcccugcccu ugacucagag cgaggaguua gcggaacagg uguacgauga gauacuggac     3780

caggucaugc auuacuuccc ucucuaugau acaaaucaau uucgugcuaa gcugucagcc     3840

gguaaagcag ccuucaugaa acuuccaugg aagaguaucu gggacggcaa caaaaugacc     3900

cagauugggc agcagaucau ucuggaucgg aucuugauag gacuacacgc uaaugcagcc     3960

guuuccgacc ugggcgugcu caagauuucu acuccccugg guaaaaugca acagccgagc     4020

gggaucuccc uuucaccuga uacccagauc auuuaccaga gcccaacagg cuuauuugaa     4080

agaaggguag cgcugcgcga ccucuga                                         4107


<210>  3112
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3112
augguggaga agaaaagcua cuccaucggc cuggacauug ggaccaacuc ugucggaugg       60

gccguuauaa cagaugacua uaaggugccc gcuaaaaaga ugaaaguacu cgguaauacu      120

ucaagacaga guaucaagaa gaacaugauu ggcgcacuuc uguucgauga agggggaccu      180

gccgcgagca cgagggugaa acggaccaca cgccgacguu acacuagaag gaagaaucgg      240

uugugcuauu uacgcgacau cuuugagucc gaaaugcaca ccauugauaa acauuucuuu      300

cugagacuag aggacucuuu ccuccacaag ucggauaaaa gguacgaagc ucauccaauc      360

uuuggcacac ugcaagagga gaaggccuau cacgacaacu acccgaccau auaucaucuu      420

cggaaggcac uggccgauaa uacugaaaaa gcugacuugc gccucaucua ccuggcacug      480

gcccacauua ucaaguuccg aggucacuuu cucauugagg gggcuuuauc agccaacaau      540

acagaugucc agcagcuggu gcaugcgcuu guugacgcau acaacaucau guucgaagag      600

gaucaguugg acauagaggc cauugacgug aaagcuaucc ugaccgaaaa gauuagcaaa      660

acgagaaggc uagagaaucu caucaguaac auccccggac aaaagaaaaa uggccuguuu      720

ggcaaccuua uugcccuguc cuuggggcuc acuccuaauu ucaagucuca cuuuaaccug      780

ccagaagaug caaaguuaca gcuggcuaaa gacaccuaug augaggaacu caauaaccuu      840

cugacacaga uaggagacga guacgccgau uuguuccuaa gcgcgaagaa ccuguccgac      900

gccauccucc ugucagauau ucuuacuguc aauggugacg gcacccaggc accccugagu      960

gcuagcuuga ucaaacggua ugaggaacau cgucaagauc ucgcccuguu aaagcagaug     1020

uuuaaagagc agcugccuga ccuauaccgc gauguguuca cagacgaaaa caaggacggg     1080

uaugcuggau acauaucugg caagaccucc caggaggccu ucuauaaaua cauuaagcca     1140

auccucgaaa cgcuggaugg ugcagaggac uuucuuacua aaaucaauag agaggauuuc     1200

uugaggaagc aacggacauu ugacaacggg ucaauucccc accagaucca ccugggagaa     1260

cuccaggcca uucuggagcg acaacaggcu uacuauccuu uccuuaaaga uaaucaggaa     1320

aagauagaga agauccugac cuuucgcauu ccauacuaua ucggcccguu ggcgagaggc     1380

aacagcaggu ucgcauggcu cacucggaca agugaccaga aaaucacccc cuggaauuuu     1440

gaugaaaugg uagaccaaga ggccucugcu caggccuuca uugagcgcau gacgaacuuu     1500

gaugaauacc ugccucagga gaagguuuua ccaaaacauu cccugaccua ugaauacuuc     1560

acaguguaua augagcuaac uaaggucaaa uacgugaccg aggggaugac aaagcccgaa     1620

uuucucagcg caggacagaa ggagcaaaua guggaacugc uuuucaaaaa guacagaaaa     1680

gucacuguua agcaguugaa agaggacuuu uucucgaaga ucgaauguuu cgauaccgug     1740

gacauuucug guguagagga uaaauuuaac gccucccugg gcacauauca cgaccuccug     1800

aagaucauua aggacaaggc uuucuuagau aauucagaga acgaaaauau cauagaggac     1860

aucauucuga cccuuacuuu guuugaagau aaagagauga ucgccaacag gcucgcagug     1920

uacgaagacc uguucgauca gaauguccua aaacagcuga agcgucggca uuauacgggg     1980

uggggacgac ucaguaaaca acugauuaac ggcaugagag acaagcacac agguaagacc     2040

auccuugauu uucuaaaagc ggacggguuc auaaacagga auuuuaugca gcugauuaac     2100

gaugacaauc ugagcuucaa ggaggagauc aaaaaggcuc aggaaggcgg acucaaagau     2160

uccauuaacg accagauccg cgaucuggcc ggcucuccug caauaaagaa ggggauccuu     2220

caaacuauua auaucgugga cgagauuguu aaaaucaugg gaaaggcccc acagcauaua     2280

guggucgaaa uggcucggga cgugcagaaa accgauauug guguaaagca aagcagagag     2340

aggaugaaac gcguucagga agugcugaag aaguugggcu cacagcuccu gaaagagcac     2400

cccgucgaga acuuucagcu acaaaaugaa cgacuguacc ucuauuaccu gcagaacggg     2460

aaggacaugu auacaggaga ggaacuuagu aucuccaauu ugagccacua cgauaucgac     2520

cauauuaucc cgcggucuuu cauuaaagau gacucaauag auaacaaggu gcugacccgu     2580

agugagcaca auagaggcaa aacugacaac gucccuagca ucgagguggu uaagaggaug     2640

aaacccuacu ggcagaaguu acucgauaca aaggugauuu cccagcgcaa auuugacaau     2700

cugacgaagg ccgaacgggg ugggcuucaa gagucugaua aagcaaacuu cauccagaga     2760

cagcugguag aaaccaggca gauuacuaag caugucgcuc aaauccuaga cucgcgauuu     2820

aauacagagc gcgacgaaaa agaucggcca auaagaaggg ugaagguuau cacccucaag     2880

agcaaauucg uguccgacuu ccgucaggau uuuggcuucu auaagcugcg cgagauuaac     2940

gacuaccacc acgcccauga ugcguauuug aacgccgucg ugggaacagc ucuguuaaaa     3000

auguacccua agcucgcauc agaguuugua uauggcgacu accagaaaua ugaucugaag     3060

cggauggugg ggaagagugg aaaagccucu ggucacgcua cugccaagua cuucuuuuac     3120

agcaaucuua ugaacuucuu uaaauccgaa guuaagcugg caaauggcaa caucauuaaa     3180

agaucaccca ucgaggucaa ugaagagacc ggggaaauag ugugggacaa gacgaaggau     3240

uucggaaccg ugaggaaagu cuugagcgcg ccacaaguua acauugugaa gaaaacagag     3300

auccagacug gcgguuuuuc uaaugagacc auccucucca aggggaaaag uagcaagcug     3360

auuccccgaa agaacaaaug gcgggacaca acuaaguaug gcggauucaa uaccccuacg     3420

guggccuacu cuguauuggu cguggcuaaa guugaaaagg gcaaagccaa gaaacugaag     3480

ccagugaagg agcuugucgg gauaacaauc auggaacgca ccaaauuuga ggcaaacccg     3540

auugcuuucc ucgaauccaa gggauaucau gauauccagg agcaccugau gauuacuuug     3600

cccaaauacu cacuguucga guuagaaaau ggcagaaggc ggcuccuggc cagugcaaca     3660

gagcuucaga aggguaacga aauggugcug ccucaacauu ugguuaccuu ucucuaucga     3720

gugagcaaaa gagacaaggg gacucagucg gagaauaugg aguacaucuc caaccacaag     3780

gaaaaauuca uagagaucuu ucacuauauu auccgcuacg ccgaaaagaa uguaauuaaa     3840

ccaaagguca ucgagaggcu gaacgauacc uucaaccaga aauuuaauga cucugaccua     3900

acagaacuga gcauaucauu ccuuaaccuc uuuaaguuca cgaguuuugg agcucccgag     3960

aaguucaccu uuuuaaauuc cgagauuaaa caggaugacg ugcguuaccg gucuacuaag     4020

gaaugcuuga acagcacauu aauccaucaa uccgugaccg gccuguauga gacaagaauc     4080

gaucucucac aguucggugg cgacuga                                         4107


<210>  3113
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3113
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggaucugc      240

uaucugcagg agauuuucag caacgaaauc gcuaaaguag acgauuccuu uuuccaucgc      300

cuagaggaau cuuuucucgu ggaggaggac aagaaacacg aaagacaucc uauauucgga      360

aauaucgucg augagguggc cuaccacgaa aaguauccaa ccauuuacca ucugaggaag      420

aaacuugcag acucgacaga uaaggccgac cugcgguuga ucuaucucgc ucuggcacac      480

augauuaaau uucgcggcca cuuccugauc gagggugauc ucaacccgga caauucagac      540

guugauaagu uauuuauaca acuggugcag accuacaacc agcuuuucga ggaaaauccc      600

auuaacgcca gccgagucga cgcuaaagcc aucuugagug cgagacuguc caagucuagg      660

cggcuagaga aucucauugc acagcugccu ggggaaaaac guaacggacu uuuuggcaau      720

cugaucgccu ugagccucgg ccugacucca aacuucaagu ccaauuuuga uuuagcugag      780

gacgccaagc ugcaacucuc aaaagauaca uacgacgaug accuugauaa ccuguuggca      840

cagaucgggg accaguaugc ugaucuauuc cuggccgcga agaaccucag ugacgccauu      900

cugcuuagcg acauacugcg cgugaauacc gaaaucacga aagcacccuu gucugcuucc      960

augauuaaga gauacgauga gcaucaccag gaccucacuc uguuaaaagc ccugguaagg     1020

caacagcuac cugagaagua uaaggaaauc uucuuugauc agucaaagaa cggauacgcu     1080

gguuauauag acggcggggc cagccaggag gaguucuaca aguuuauuaa accaauccuc     1140

gagaagaugg auggaaccga agaacugcuu gcaaaauuga aucgggagga ccugcuccga     1200

aagcaacgca cauucgauaa cggcaguauc ccccaucaga uucaccuggg ugaacuucac     1260

gccauccuga gaaggcagga ggacuuuuau ccuuucuuga aggauaaucg ggagaaaauu     1320

gaaaagauac ucacuuuucg caucccauac uacguugggc cgcuggcuag aggaaacucu     1380

agguucgcgu ggaugacccg uaaauccgaa gagacaauua cccccuggaa uuuugaggaa     1440

guggucgaca agggcgcaag cgcccaaucg uucaucgagc ggaugacgaa cuuugauaaa     1500

aauuuaccua acgaaaaggu gcugccaaag cauucucuac ucuaugagua cuucacugug     1560

uauaaugagc ugacaaaagu caaguacguu accgaaggca ugcgaaaacc cgcuuuucuu     1620

uccggggagc agaagaaagc caucguggac uugcuguuca agacuaacag aaaaguaaca     1680

gugaagcagc ucaaggaaga uuauuucaaa aagauugagu guuuugacuc agucgaaaua     1740

aguggagugg aggacagguu caaugcaagc cuggguaccu accacgauuu acugaaaauc     1800

auuaaggaca aagauuuucu ugacaacgag gaaaaugagg auaucuugga agacauuguu     1860

cucacgcuga cccuauucga ggaucgcgaa augaucgagg agcggcugaa gacauaugcc     1920

caucucuuug acgauaaggu gaugaaacag cugaagagaa ggcgcuacac uggcuggggg     1980

cgacuuuccc ggaaauugau aaacggaauc cgugacaagc aaucuggcaa aaccauucug     2040

gauuucuuaa agagcgacgg uuuugcuaac agaaauuuca ugcagcucau ccacgaugac     2100

ucacugacau uuaaggaaga ccuucagaaa gcccagguca gugggcaagg cgauucccug     2160

caugagcaca uugcaaacuu ggcgggaagc ccugcuauca agaaaggcau acuccagacu     2220

gugaagguag uugacgaacu ggugaaaguc auggggaggc acaagccaga gaauauugug     2280

aucgaaaugg cccgcgagaa ccagaccaca cagaagggac agaaaaauuc ucgggagaga     2340

augaagagga uugaagaggg uaucaaagaa cuaggcucag auauacugaa ggaguacccc     2400

gucgagacca cucaacucca gaacgaaaaa cuguaucuuu acuauuugca gaaugggcga     2460

gacauguacg uggaucaaga gcuggacauc aaccgcuuaa gugauuauga cguugaucau     2520

auugugccgc agagcuuccu caaggacgau uccaucgaca auaaaguacu gacgcggucu     2580

gacaagaaca gaggaaaguc ggauaauguc ccuagcgaag aggugguuaa aaagaugaaa     2640

aacuacugga agcagcuucu gaaugcaaaa cuaauuacac aaaggaaguu ugacaaccuc     2700

accaaggccg aacguggcgg ucuguccgag uuggauaaag cuggguucau caagcgccag     2760

cugguggaga cucggcagau aaccaaacac gucgcccaga uuuuagacuc aagaaugaau     2820

acaaaguaug augaaaacga caaacucauc agggaggugc gaguaaucac ccugaagagu     2880

aaacuugugu cugauuuucg gaaagacuuc caauucuaca agguucgcga aauuaacaau     2940

uaccaucacg cacacgaugc uuaucugaac gccgucgugg gcacugcguu gaucaaaaag     3000

uaccccaaac ucgagagcga auuuguguau ggagacuaca aggucuauga uauuagaaag     3060

augauagcca aauccgagca ggagaucggc aaggcuacag caaaauacuu cuuuuauuca     3120

aauauuauga acuucuuuaa gacggaaauc acccuggcca auggggagau uaggaaacgg     3180

ccacuaaucg aaacuaacgg agagacaggu gaaauaguuu gggacaaggg ccgagauuuc     3240

gcuaccguga gaaaggugcu gagcaugccu cagguaaaua ucgucaaaaa gacagaggug     3300

cagacugggg gauuuucuaa agaguccauu cuucccaagc gcaacaguga caaacucauc     3360

gccaggaaga aagacuggga uccaaagaag uacggcgguu ucgacagccc caccguugca     3420

uacucugugc uggucguggc gaaaguugaa aaggggaaau ccaagaaauu gaagucagug     3480

aaggagcugg uaggcauuac gaucauggaa cguaguagcu uugagaaaga uccugucgac     3540

uucuuagagg ccaagggaua uaaagaagug cggaaggauc ucauaauuaa acugccaaag     3600

uacucgcuuu uugagcugga aaauggcaga aagaggaugu uggcuuccgc cggggagcuc     3660

caaaaaggaa acgaacuggc acuaccgucu aaguauguga auuuccugua ccuugcuagc     3720

cauuaugaga aacucaaggg cucacccgag gacaacgaac agaaacagcu guucgucgag     3780

cagcacaagc auuacuugga ugaaaucauc gagcaaauua gugaauuuuc caagcgcguu     3840

auauuagccg acgcaaaucu ggauaaagug cucucugccu auaacaagca ccgagacaaa     3900

ccuaucagag agcaggcuga gaauauuauc caucuguuca cccuuacaaa ccugggugcg     3960

ccagccgcau uuaagugcuu cgauacuacc auugggcgga acagguacaa aagcaucaag     4020

gaaguauugg acgcuacacu aauacaccag uccaucacug gacuguacga gacccgcauu     4080

gaucucucac aacugggcgg ugacuga                                         4107


<210>  3114
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3114
auggacaaga aauacagcau cggccuggau auugggaccu ccucuauagg auucgugggu       60

gucgacgaga acggcuggcc ccucaauauc aaggggaaaa gagccauugg agcucaccuu      120

uuuagggaag gcgaggcagc cgcggaaaca cgggguuucc gcacuacgcg acguagauau      180

aacaggcgga aguggcgccu gagauuguua agggagaucu uugaugaacc uauuucacgg      240

guugacccaa ccuucuuugc ucgcaugaaa gagaguagcg uguccccgaa ggaucccaau      300

uacgacgggc uggccaagga gauccuaaua ccucgagaua aagacggaaa gauccucuau      360

ccaacaauuu accaucugag acacgcacuu auguaugaag aucaucaguu cgacaucagg      420

gagauuuacc uggccaucca ccauauagua aaauaucggg gccacuuuuu gcucgauggc      480

gcugcaaaga acuucgaauc uucgaaucug gaccugaaau cagauauuca cgagaucaac      540

gccauucucg uggggaucuu uggaaaugag caaguccagu ucaacgacca guacguggaa      600

aaguuaaagg agguucugcu ugacagcacu cagagucguu ccgaucgcca aaaagcugcc      660

gcgaaguugc ugcuacccac caaugaccuc gauaaagaca caaccaagau ccugaaaacu      720

gugucuacag aaauuaccaa ggcaauaacg ggucuuaaag ccaaguuuuu cgucaucacu      780

ggcaacgaua uugcuaagga ccagcagaaa gauuggaccu uuagcaucga cacacuggau      840

gagaagaugu ccgugauaga accuucauuc agugagaaaa auuucagcuu gauuacuauc      900

cuccagaagc uguacucuuc cguaaccuua gccgggaucg uuccaaacgg acugucacuc      960

agcgaggcaa ugauucaaaa auaugacaga caugcuagug aucuugugcu guugaagaag     1020

uacugggaca caaaacccaa gguccuaaaa caccagcugc agaauaccua ugccgaauac     1080

aucgagauuc aucagguggu ggacgaauuu uauucugauc ucuccaagcu gauuaaaaac     1140

gacgaugaca cgcuugcgca agagaucaag agcaagauua cuaucggcaa uuuccugccu     1200

aaacagagga caaccgaaaa cggugucauc ccauaccagu ugcaacagcg ggagcucgau     1260

acuauuauaa agaaccagca ggaguauuac ccguggcugg ccgaagagaa ucccguugca     1320

gaccaccaaa ucgcuuuucc uuacaaauua gaugaacugg ugggguuccg aauaccauau     1380

uacguaggac ccaugaucgc caaggaggac acaucgaacu cuaaaaauuc caaguuugcu     1440

uggaugauuc gcaagcaguc aggcgaagug accccuugga acuucgauga gaaaaucgac     1500

agaacggcca gugcaaccgc cuuuauagau aggaugacaa cuaccgacac auaucuaauc     1560

ggcgaggaug uccucccaaa gcagagccug auuuaccagc gguucgaagu gcuuaaugag     1620

uugaacaaaa ucuccauuga cgggcaaccc aucacucgca agcagaaaca gucuguuuau     1680

acccacgugu uugaaaagaa aaagacaguc caggugaagg acguacaaaa auuccugguu     1740

agcucaggag aggugaugag ucucccgaag auagguggcc uggcuaauga gaaacaguuu     1800

aacuccagcc uuucuacccu guacgauuau aauaagauua ucccugacgc guuggaagau     1860

agaaacaaag ucgacgauau ugagaagauc auacucuggu caacuaucuu cgaagacaau     1920

aguauuuuug uggagaagcu ggauagcauc gacugguuaa cguccucuca gaggaaacgu     1980

cuggcacaga agcgguaccg agggugggga agacuaucgc ucaggcugcu uacaaccuuc     2040

aaaaacaagg auggcuacag cauuuuggac gaacugugga acuccaauca uaauuucaug     2100

gagauccaag cccagucaga uuuuagugag cagauagcua cugccaacaa caauaaccuc     2160

ucucaagucc ugcccgcaaa augggaacca ggucgcaaga auuuagccag ccuggacgag     2220

uucauuucca accuuuauac cucaccucag aauaagaaag cucuccggaa ggccaucaga     2280

gugguucagg auaugaugac agcagcgggg cacaugcccg aaaagaucuu uauugaggug     2340

gcuagggaca ccagcaacuc ucaucagcaa acuaucgccc gccagcgaca gcuccagcaa     2400

cuguaccagu ccgaagacaa ggagguaauu gauaguagcg uccggucuga gcuagacgau     2460

auacugaaaa aucgugcaaa caucaaggac caguuguucc uguauuuuca gcaaggcgga     2520

agagaucuuu acacaggcaa ggccauuaau aucgacaaau ugucccugua ucacauugau     2580

cauaucuuac cacagucacu cgugaaggac gauagucugg acaacagggu ucuugugagc     2640

cagaaaacga augaagauaa gggggucacc uucgugucgg acaaauacau gaacaauaug     2700

cugcccacuu ggaagcgcug gcacgagaug ggauugauau ccgcucggaa acucaagaac     2760

cugacaauga gaccugacga aaucgauaag uaugccccag guuuuauuca aaggcagcua     2820

guagagaccc gacaggugac aaaacugguu aucgaacucc uggacgcacu uuugggcaau     2880

ucucagacug agauuaucac cauaaaggcu agccugucac accaauuccg ccagcaguuu     2940

gauuuaguca aaauucggga ggugaacgac aaccaucacg ccuucgaugc guuucucgcc     3000

gcuagaaucg ggacguaccu guacaagagg uauccgaaac uucgucccua cuucguguau     3060

ggacaguaca ugcaaaauca uaagaaccug aagaguauca aucuaauuaa aacccucgac     3120

uccgauaagg caauagucga cgaaggcaca ggugagaucc ugugggauaa aaagucugac     3180

uuggccaaaa ugauuaagau cuauaacuac cagcgccugu uaauuacuca cgaaagccgg     3240

accuccuuug gggcuaugua uaagcagaaa auccucaagg ccacagaugc aaaacagaau     3300

ggcggaggcu ucaagcuggu uccuacuaaa agaaacaggc caguggacau auacggggga     3360

uacaccgguc aaacggcggc cuaucuuaca aucgugcgag uauacucaaa gaagaccagc     3420

gagcugcggg ucuugcccau uuauacuaau aaagugcagc agaucgagaa ggcuaugaca     3480

cagggcgaua aagccaccau gcucgcauuc aagaaaguua uugcuccuca acuggggaag     3540

gaaaacaaga aaacucacga gaucaagaua ccagaauuug acguggucau ucccaaagug     3600

cuacuggacc agcuuguuau cgaugacauc gccggaaguc cgcaucgcuu cagacucggc     3660

accgauacau acuaucacaa uucucagcaa cuguuuuugc cucuguccau gcagaggcag     3720

uuaauggacc gggagagcga ugacaaggug uaccucgaua uuuucaacga cguacugaaa     3780

caggucgauu cguauuuuaa ucuuuacgac gugaacgguu uccgagcagc ccugcaaaag     3840

ucuaaaaagg cuuuugaaca guugccaauc aaugauuucc ccaaggacgu gccugauaac     3900

aaaaagaaaa aggggaaaag aucaauacuc aaggagauuu uuaucggccu gcaugcgaau     3960

gccacgcuag gaaaccugaa gcagaugggc auuaaaaccg acuucgggaa acuucagaaa     4020

aguaacggac uccgcaucac ugagaauugc aagauaauuc accaaucccc cacaggccug     4080

uuugaaagga ucgucccaau caaauga                                         4107


<210>  3115
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3115
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gaucgcagaa      180

gccacgaggu uaaaacggac cgcgcgccga cguuacacaa gaaggaagaa ucggauuugc      240

uaucugcagg agaucuucag caacgaaaug gcuaaaguag acgauuccuu uuuccaucgc      300

cuagaggaau cuuuucucgu ggaggaggac aagaaacacg aaagacaucc uauauucgga      360

aauaucgucg augagguggc cuaccacgaa aaguauccaa cuauuuacca ucugaggaag      420

aaacuuguug acucgaccga uaaggcagac cugcgguuga ucuaucucgc ccuggcucac      480

augauuaaau uucgcggcca cuuccugauc gagggugauc ucaacccgga caauucagac      540

guggauaagu uauuuauaca acugguccag acauacaacc agcuuuucga ggaaaauccc      600

auuaacgcaa gcggggugga cgccaaagcu aucuugagug cccgacuguc caagucuaga      660

aggcuagaga aucucauugc gcagcugccu ggagaaaaga agaacggccu uuuuggcaau      720

cugaucgcau ugagccucgg gcugacccca aacuucaagu ccaauuuuga uuuagccgag      780

gacgcuaaac ugcaacucuc aaaggauacu uacgacgaug accuugauaa ccuguuggcc      840

cagaucggag accaguaugc agaucuauuc cuggcugcca aaaaccucag ugacgcgauu      900

cugcuuagcg acauacugcg gcucaauucu gaaaucacaa aggcccccuu guccgcauca      960

augauuaaac guuacgauga gcaucaccag gaccugaccu uacugaaggc ucuaguacgc     1020

caacagcucc cugagaagua uaaagaaauc uucuuugauc agagcaagaa cgguuacgcc     1080

ggcuauauag acgggggagc uagucaggag gaauuuuaca aauuuauuaa gccaauccug     1140

gagaaaaugg auggcacgga ggaacuuuug gccaagcuga auagagagga ccuccugagg     1200

aagcaacgga cuuucgauaa cgguucuauc ccccaucaga uucaccuugg ggaacugcac     1260

gcaaucuugc gacgccagga ggacuuuuau ccuuuccuca aagauaauag agaaaagauu     1320

gagaaaauac ugaccuuuag gaucccauac uacguuggac cguuagcccg gggcaacucc     1380

cgcuucgcuu ggaugacaag aaagagcgaa gagacuauua cccccuggaa uuuugaggaa     1440

guggucgaca aaggcgcguc ggcacaaucu uucaucgaga ggaugacaaa cuuugauaag     1500

aaucugccua acgaaaaggu gcuaccaaaa cauucccucc uguaugagua cuucaccgug     1560

uauaaugagc uuacgaaggu caaauacguu acugaaggga ugcguaagcc cgccuuuuug     1620

ucaggagagc agaaaaaggc uaucguggac cuccuguuca aaacaaaccg gaagguaacc     1680

gugaagcagc ugaaagaaga uuauuucaag aaaauugagu guuuugacag ugucgaaaua     1740

agcggugugg aggaccgauu caaugccucc uuaggcacuu accacgaucu gcuuaagauc     1800

auuaaagaca aggauuucuu ggacaacgag gaaaaugagg auauccucga agacauuguu     1860

cugacacuaa cccuguucga ggauagagaa augaucgagg agaggcucaa gacguaugca     1920

caucuguuug acgauaaagu gaugaagcag cuuaaacgcc ggagauacac cgucuggggg     1980

agguugucuc gcaagcugau aaacggaauc cgagacaaac aaagcggcaa gacaauuuua     2040

gauuuccuca agucagacgg uuuugccaac cggaauuuca ugcagcugau ccacgaugac     2100

agucuuacuu uuaaagaaga cauucagaag gcucaggugu ccgggcaagg cgauagccug     2160

caugagcaca ucgccaacuu ggcaggaucu ccugcgauua aaaagggcau acuccagacc     2220

guaaaaguug uggacgaacu ggucaaggug auggggcguc acaagccaga gaauaucguc     2280

auugaaaugg cuagagagaa ccagacaacu cagaaaggac aaaagaacuc aagggagcgc     2340

augaaacgga ucgaagaggg uauaaaggaa cuaggcaguc agauccugaa agagcauccc     2400

guggagaaua cccagcucca gaaugaaaag cuguaccuuu auuacuugca aaacgggaga     2460

gauauguaug uugaccagga gcuggauauu aauagguuaa gcgacuacga uguggaccac     2520

aucguaccgc aguccuucau uaaagaugac ucuaucgaua acaagguccu cacacgaucg     2580

gacaagaauc gcggaaaaag cgacaacgug ccuuccgaag agguugugaa gaaaaugaag     2640

aauuauuggc ggcaacugcu uaacgccaaa cugauaaccc agagaaaguu ugauaaucua     2700

acuaaggcag aaaggggcgg ucucucagag cuggacaaag ccggguucau uaagcgucag     2760

uuggucgaga cgcgccagau cacaaaacau guggcucaaa uccuggauag ucggaugaac     2820

accaaguacg acgaaaacga uaaauuaauu agagagguaa aggugaucac ucucaagucu     2880

aaacugguua gcgacuuuag gaaggauuuc caguucuaua aaguccgaga aauuaauaac     2940

uaccaccacg cccaugacgc auaccuuaau gcuguggugg gcaccgcccu gauaaagaaa     3000

uaucccaagu uggaguccga auuugucuac ggagauuaua agguuuacga cgugcggaaa     3060

augcucgcga agucagagca ggagaucggc aaagccacag cuaaguauuu cuuuuacagc     3120

aacauuauga auuucuuuaa aaccgaaauc acucuggcaa acggggagau ucgcaagaga     3180

ccacuaaucg aaacaaaugg agagacgggu gaaauagugu gggauaaggg cagggacuuc     3240

gccaccguac ggaaaguccu gucuaugccu caggugaaca ucguuaagaa aacugaggug     3300

caaacagggg gauuuuccaa ggagaguauu cuucccaaac gaaauagcga uaagcucauc     3360

gcucgcaaaa aggacuggga cccaaagaaa uauggcgguu ucgauucucc caccgucgcc     3420

uacuccgugc ugguuguggc aaagguagaa aaagggaaga guaaaaaguu gaagucaguc     3480

aaagagcugu uaggcauuac aaucauggaa agaagcucgu uugagaagaa cccuauagac     3540

uuccucgagg cgaaaggaua caaggaagug aggaaagauc ugauuaucaa acuuccaaag     3600

uauucccugu uugaguugga aaauggccgu aaacggaugc ucgccucugc uggggagcug     3660

cagaagggaa acgaacuagc ccugccgagc aaauacguga auuuccuuua ucucgcauca     3720

cacuacgaga agcugaaagg cagucccgag gacaacgaac agaagcaguu guucgucgag     3780

caacauaagc acuauuuaga ugaaaucauu gagcagauau ccgaauuuuc uaaaagaguu     3840

auccuggcug acgccaaucu cgauaaggug cugagcgcau acaacaaaca uagggacaag     3900

ccuauucgcg agcaggccga gaacaucauu caccuuuuca cucugaccaa uuugggugcu     3960

ccaacggcgu uuaaauauuu cgauaccaca aucgaccgaa agagauacac uuccaccaaa     4020

gaaguacuag augccacauu uauacaccaa ucaaucacug ggcuguacga gacccggauu     4080

gaccucagcc agcugggagg cgauuga                                         4107


<210>  3116
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3116
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggaucugc      240

uaucugcagg agauuuucag caacgaaaug gcuaaaguag acgauuccuu uuuccaucgc      300

cuagaggaau cuuuucucgu ggaggaggac aagaaacacg aaagacaucc uaucuucgga      360

aauauagucg augagguggc cuaccacgaa aaguauccaa ccaucuacca ucugaggaag      420

aaacuuguug acucgacaga uaaggcagac cugcgguuga uuuaucucgc ccuggcucac      480

augaucaaau uucgcggcca cuuccugauu gagggugauc ucaacccgga caauucagac      540

guggauaagu uauuuaucca acugguccag accuacaacc agcuuuucga ggaaaauccc      600

auaaacgcaa gcggggugga cgccaaagcu auuuugagug cccgacuguc caagucuaga      660

aggcuagaga aucucaucgc gcagcugccu ggagaaaaga agaacggccu uuuuggcaau      720

cugauugcau ugagccucgg gcugacucca aacuucaagu ccaauuuuga uuuagccgag      780

gacgcuaaac ugcaacucuc aaaggauaca uacgacgaug accuugauaa ccuguuggcc      840

cagaucggag accaguaugc agaucuauuc cuggcugcca aaaaccucag ugacgcgauc      900

cugcuuagcg acauucugcg gcucaauucu gaaauaacca aggcccccuu guccgcauca      960

augaucaaac guuacgauga gcaucaccag gaccugacgu uacugaaggc ucuaguacgc     1020

caacagcucc cugagaagua uaaagaaauu uucuuugauc agagcaagaa cgguuacgcc     1080

ggcuauaucg acgggggagc uagucaggag gaauuuuaca aauuuauaaa gccaauucug     1140

gagaaaaugg auggcacuga ggaacuuuug gccaagcuga auagagagga ccuccugagg     1200

aagcaacgga ccuucgauaa cgguucuauc ccccaucaga uccaccuugg ggaacugcac     1260

gcaauuuugc gacgccagga ggacuuuuau ccuuuccuca aagauaauag agaaaagauc     1320

gagaaaauuc ugacauuuag gauaccauac uacguuggac cguuagcccg gggcaacucc     1380

cgcuucgcuu ggaugacuag aaagagcgaa gagaccauca cacccuggaa uuuugaggaa     1440

guggucgaca aaggcgcguc ggcacaaucu uucauugaga ggaugaccaa cuuugauaag     1500

aaucugccua acgaaaaggu gcuaccaaaa cauucccucc uguaugagua cuucacggug     1560

uauaaugagc uuacuaaggu caaauacguu acagaaggga ugcguaagcc cgccuuuuug     1620

ucaggagagc agaaaaaggc uaucguggac cuccuguuca aaaccaaccg gaagguaacu     1680

gugaagcagc ugaaagaaga uuauuucaag aaaaucgagu guuuugacag ugucgaaauu     1740

agcggugugg aggaccgauu caaugccucc uuaggcacau accacgaucu gcuuaagaua     1800

aucaaagaca aggauuucuu ggacaacgag gaaaaugagg auauucucga agacaucguu     1860

cugacccuaa cgcuguucga ggauagagaa augauugagg agaggcucaa gaccuaugca     1920

caucuguuug acgauaaagu gaugaagcag cuuaaacgcc ggagauacac agggugggga     1980

agguugucuc gcaagcugau caacggcaua cgagacaaac aaagcgguaa gacuaucuua     2040

gauuuccuca agucagacgg guuugccaac cggaauuuca ugcagcugau ucacgaugac     2100

agucuuaccu uuaaagaaga cauccagaag gcucaggucu ccggccaagg agauagccug     2160

caugagcaca uugccaacuu ggcaggcucu ccugcgauca aaaaggggau acuccagaca     2220

gugaaaguag uugacgaacu ggugaagguc augggacguc acaagccaga gaauauugug     2280

aucgaaaugg cuagagagaa ccagacuacc cagaaagguc aaaagaacuc aagggagcgc     2340

augaaacgga uugaagaggg caucaaggaa cuagggaguc agauacugaa agagcauccc     2400

gucgagaaua cacagcucca gaaugaaaag cuguaccuuu auuacuugca aaacggaaga     2460

gauauguaug uggaccagga gcuggauauc aauagguuaa gcgacuacga uguugaccac     2520

auugugccgc aguccuucau caaagaugac ucuauugaua acaagguacu cacccgaucg     2580

gacaagaauc gcggcaaaag caacaauguc ccuuccgaag aggugguuaa gaaaaugaag     2640

aacuauuggc ggcaacugcu uaaugccaaa cugaucacuc agagaaaguu ugacaaccua     2700

acgaaggcag aaaggggugg gcucucagag cuggauaaag ccggcuucau aaagcgucag     2760

uugguggaga cacgccagau uaccaaacau gucgcucaaa uccuggacag ucggaugaac     2820

acuaaguacg augaaaauga caaauuaauc agagagguga agguaauuac ccucaagucu     2880

aaacugguga gcgauuuuag gaaggacuuc caguucuaua aaguucgaga aaucaacaau     2940

uaccaccacg cccaugaugc auaccuuaac gcugucgugg gaacagcccu gauuaagaaa     3000

uaucccaagu uggaguccga auuuguguac ggcgacuaua aggucuacga uguucggaaa     3060

augauagcga agucagagca ggagaucggg aaagccaccg cuaaguauuu cuuuuacagc     3120

aauauuauga acuucuuuaa aacugaaauc acacucgcaa auggagagau ucgcaagaga     3180

ccacugaucg aaacgaacgg ugagaccggc gaaauagugu gggacaaggg gagggauuuc     3240

gccacugugc ggaaaguacu aucuaugccu caggucaaua ucgugaagaa aacagagguu     3300

caaaccggag gcuuuuccaa ggagaguauu cugcccaaac gaaacagcga caaacuuauc     3360

gcucgcaaaa aggauuggga cccaaagaaa uauggugggu ucgacucucc cacaguggcc     3420

uacuccgucc ucgugguugc aaagguggaa aaaggcaaga guaaaaagcu gaagucagua     3480

aaagaguugc ugggaauuac uaucauggaa agaagcucgu uugagaagaa uccuauagau     3540

uucuuagagg cgaaaggcua caaggaaguc aggaaagacc ucauugugaa gcugccaaag     3600

uauucccuuu uugagcugga aaacgggcgu aaacggaugu uggccucugc uggagagcuc     3660

cagaagggca augaacuggc ccuaccgagc aaauacguga acuuccugua ucuugcauca     3720

cacuacgaga agcucaaagg uagucccgag gauaaugaac agaagcagcu guucgucgag     3780

caacauaagc acuauuugga cgaaaucauc gagcagauuu ccgaauuuuc uaaaagaguu     3840

auauuagcug augccaaccu ggacaaggug cucagcgcau acaacaaaca uagggauaag     3900

ccuauccgcg agcaggccga gaauauuauc caccuguuca cccuuacgaa ccugggggcu     3960

ccagcggccu uuaaauauuu cgacaccaca auugaucgaa agagauacac uuccaccaaa     4020

gaaguauugg acgcaacauu uauccaccaa ucaauaacug gacuauacga gacccggauc     4080

gaucugagcc agcucggcgg ugacuga                                         4107


<210>  3117
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3117
augagcagac ccuacaacau cggccuggac auugggaccu ccucuauagg auggucagug       60

gucgaugacc agaguaagcu cguuagcgug agggguaaau auggcuacgg gguacggcuu      120

uaugaugagg gacaaacagc ugcugaacgc cgauccuucc guacuacgag aaggcggcug      180

aagcgcagaa aauggagguu gggcuuacug cgggagaucu uugaaccuua cauuacccca      240

aucgacgaua cauucuuucu acgccagaag cagucuaauc ucucgccgaa agaccagcga      300

aagcuguaua cucaaaccuc acuuuucaac gauagaacag acagggcauu uuacgaugac      360

uaucccacca uuuaccaccu ucgguaccgu uugaugacug agaagcgcca guucgauauc      420

agagaaauau aucucgccau gcaucacauc gugaaauaca ggggucauuu ucugaaugag      480

gcgccuguca gcaguuucaa guccucugag auuaaccugg uggcucacuu ugaccggcuc      540

aauacaaucu ucgccgauuu auuuagcgaa uccggguucc agcuggagac cgacaaacuu      600

gcagaaguua aggccuugcu gcuagacaac cagcaaucag cuaguaaucg acagcgccag      660

gcacucagcc ugauuuauac gccaucuacu aacaaagccg uggagaagca gaauaaggcu      720

aucgccaccg agcuucugaa agcgauauug ggacucaagg caaaauuuaa cguccugaca      780

ggcauugaag ccgaggaugu gaaggcuugg acuuuaaccu ucaaugccga aaacuucgac      840

gaggaaaugg uaaaacugga guccucacuc gaugacaaug cacaucaaau cauugagagc      900

cuucaggaac uguacagugg cguuuugcua gcugggaucg ugcccgagaa ccagucucug      960

ucccaagcca ugaucacaaa guaugaugac caccagaaac accucaagau gcugaaggcg     1020

gucagagaag cccuugcacc uggagauagg cagcggcuga aacaggcuua cgaccaauau     1080

guggaugguc aggagaauac caaggccuac agcaaagaag acuuuuacgg cgauauuacg     1140

aaggcuuuga aaaacaaccc agaccauccc auagugucgg agaucaagaa gcucauugag     1200

cuggaccagu ucaugccuaa acagcgcacu aaggauaaug gggccauccc acaccaauua     1260

caucagcagg aacuggacag aauaauugag aaccagcaac aguauuaccc guggcuagca     1320

gaacucaauc ccaacucuaa aaggcagaca gucgccaagu auaaacugga ugagcuuguu     1380

gcuuuucgug ugccuuacua uguaggacca uugaucaccg cggaacagca acggcagucc     1440

ucagacgcaa aguucgccug gcugauccga aaggcugagg gcagaauuac ucccuggaau     1500

uuugaugaca aaguggauag gcaggccagu gcaaacgagu ucaucaagcg caugacaacc     1560

acggacaccu accuccuggc cgaagauguc cuuccuaaac aaagccugau uuaucagcgg     1620

uuugaggugu ugaaugaacu caacggucug aagauagacg aucagccaau cacaacugag     1680

uuaaaacagg cuauuuucac cgaccuguuu augcaaaaga ucuccguuac agugaagaau     1740

auccaggauu accuagucuc ugagaaaaga uacgccagca ggcccgcaau uacugggcuc     1800

ucagacgaaa acaaguucaa uagucgccug uccaccuauc acgaccuuaa aaugauagug     1860

ggagaugcgg uagacgaugu ugacaagcag gcugauuugg agaaaugcau cgaauggagc     1920

acaauuuuug aggacggcaa gaucuacucu gccaaacuga acgaaauuga uuggcucacc     1980

gaccagcaac gagugcagcu ggcagccaag cgguaucgug gcugggggag auuaucagcu     2040

aagcugcuua cucagaucgu caaugccaac ggacagagga uaauggauuu gcucugggac     2100

acgacagaua auuucaugcg caucgugcac agugaggacu uugauaaacu gauuaccgag     2160

gcaaaccaaa ugaugcuagc ugaaaacgac guccaggacg ugaucaauga ucuguacacu     2220

agcccgcaga acaagaaagc ccuccggcaa auucugcuug uugugaauga cauccagaag     2280

gcgaugaaag gucaggcccc ugagagaaua uugauugagu ucgcuaggga ggaugaagua     2340

aacucccgac ugucugucca gcgcaagcgg caaguggagc agguuuauca gaauaucucg     2400

aacgaguuac ucaauaacac cgaaauuaga aaugagcuga aggaccuuag caacuccgca     2460

cugucaaaua caagguuguu ccucuacuuu augcagggcg ggcgugauau guauaccgga     2520

gacagucuga acaucgaucg ccuaucuacu uacgacauag aucauauccu gccccagagc     2580

uucauuaaag acaauucccu cgauaaccgg gugcuggucu cacaaaagau gaauagaagc     2640

aaagccgacc aggugccaac agacuuuacg ucuguagaac uuggccagaa gaugcaaauc     2700

cagugggagc agauguugag ggcuggucug auuaccaaaa agaaguacga uaacuuaacu     2760

cucaacccug accacaucuc caaauaugcc augaaggggu ucauaaaucg acagcuggug     2820

gaaacacggc aaguuauuaa acuugcaacc aaccugcuaa uggagcagua cggcgaggau     2880

aauaucgaac ucaucacagu caagagugga cugacucauc agaugcgcac cgaguuugac     2940

uuccccaaaa acagaaauuu gaacaaucac caucacgcgu uugaugccua ucugacggcu     3000

uucgugggcu uauaccuccu gaagagguau ccaaagcuga aacccuacuu uguguauggg     3060

gaauaccaaa aggccagcca gcaggacaaa uggcggaacu ucaauuuucu gaacggauug     3120

aagaaagaug agcucgucga cgagaauacc gaagcaguua uuugggauaa ggagucuggu     3180

cuggcuuacc uaaacaaaau cuaucaguuc aagaagauuc uggugacacg aggcgugcac     3240

gaaaauuccg gggcccuuuu uaaccaaacu cucuacgcag ccaaagacga uaaggcuuca     3300

ggacagggcg guaaacagcu gauaccugcg aagcaggaca gaaguaccgc cuuguauggg     3360

ggcuacagcg gaaaaacagu agcauauaug uguaucgucc gcauuaagaa uaagaaaggc     3420

gaucuguaca aggugugcgg gguugagacu ucgugguuag cucaacucaa acagcugacc     3480

gacgaagacu cccagaaggc cuuccuugag cagaaaaucu cuccacaauu cacgaaggug     3540

aagaaacaga agggaaacau uaucgaggcc gucgaagagu uugaagugau agcuccgcau     3600

auccugauua accagagguu cuuugauaau ggucaagagu ugacacucgg cagcgcaacc     3660

uauaaacaca acgaacagga gcugauccua gacaagacug ccguuaaacu gcuuaauggg     3720

gcgcuccccc ugacacaguc agaggaauug gcugaacagg uguacgauga aauuuuagac     3780

caaguaaugc auuacuuccc ucuguaugau accaaccagu uucgugccaa gcucaguacu     3840

ggaaaggcag ccuucaugaa acugccaugg aagucccagu gggacggcaa uaaaaugguc     3900

cagguggguc aacaggugau ccuugaucgg auacugauug gguugcacgc uaacgcagcc     3960

augucugacc uaaagguccu gaaaaugacc acacccuuug gcaugaucca gcagagcacg     4020

ggaaucaccc ucuccgcgga uacucaaauu auauaccagu caccuacagg ccuguucgag     4080

agaaggguug cccuucgcga cuuauga                                         4107


<210>  3118
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3118
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggaucugc      240

uaucugcagg agauuuucag caacgaaaug gcuaaaguag acgauuccuu uuuccaucgc      300

cuagaggaau cuuuucucgu ggaggaggac aagaaacacg aaagacaucc uaucuucgga      360

aauauagucg augagguggc cuaccacgaa aaguauccaa ccaucuacca ucugaggaag      420

aaacuuguug acucgacaga uaaggcagac cugcgguuga uuuaucucgc ccuggcucac      480

augaucaaau uucgcggcca cuuccugauu gagggugauc ucaacccgga caauucagac      540

guggauaagu uauuuaucca acugguccag accuacaacc agcuuuucga ggaaaauccc      600

auaaacgcaa gcggggugga cgccaaagcu auuuugagug cccgacuguc caagucuaga      660

aggcuagaga aucucaucgc gcagcugccu ggagaaaaga agaacggccu uuuuggcaau      720

cugauugcau ugagccucgg gcugacucca aacuucaagu ccaauuuuga uuuagccgag      780

gacgcuaaac ugcaacucuc aaaggauaca uacgacgaug accuugauaa ccuguuggcc      840

cagaucggag accaguaugc agaucuauuc cuggcugcca aaaaccucag ugacgcgauc      900

cugcuuagcg acauucugcg gcucaauucu gaaauaacca aggcccccuu guccgcauca      960

augaucaaac guuacgauga gcaucaccag gaccugacgu uacugaaggc ucuaguacgc     1020

caacagcucc cugagaagua uaaagaaauu uucuuugauc agagcaagaa cgguuacgcc     1080

ggcuauaucg acgggggagc uagucaggag gaauuuuaca aauuuauaaa gccaauucug     1140

gagaaaaugg auggcacuga ggaacuuuug gccaagcuga auagagagga ccuccugagg     1200

aagcaacgga ccuucgauaa cgguucuauc ccccaucaga uccaccuugg ggaacugcac     1260

gcaauuuugc gacgccagga ggacuuuuau ccuuuccuca aagauaauag agaaaagauc     1320

gagaaaauuc ugacauuuag gauaccauac uacguuggac cguuagcccg gggcaacucc     1380

cgcuucgcuu ggaugacuag aaagagcgaa gagaccauca cacccuggaa uuuugaggaa     1440

guggucgaca aaggcgcguc ggcacaaucu uucauugaga ggaugaccaa cuuugauaag     1500

aaucugccua acgaaaaggu gcuaccaaaa cauucccucc uguaugagua cuucacggug     1560

uauaaugagc uuacuaaggu caaauacguu acagaaggga ugcguaagcc cgccuuuuug     1620

ucaggagagc agaaaaaggc uaucguggac cuccuguuca aaaccaaccg gaagguaacu     1680

gugaagcagc ugaaagaaga uuauuucaag aaaaucgagu guuuugacag ugucgaaauu     1740

agcggugugg aggaccgauu caaugccucc uuaggcacau accacgaucu gcuuaagaua     1800

aucaaagaca aggauuucuu ggacaacgag gaaaaugagg auauucucga agacaucguu     1860

cugacccuaa cgcuguucga ggauagagaa augauugagg agaggcucaa gaccuaugca     1920

caucuguuug acgauaaagu gaugaagcag cuuaaacgcc ggagauacac agggugggga     1980

agguugucuc gcaagcugau caacggcaua cgagacaaac aaagcgguaa gacuaucuua     2040

gauuuccuca agucagacgg guuugccaac cggaauuuca ugcagcugau ucacgaugac     2100

agucuuaccu uuaaagaaga cauccagaag gcucaggucu ccggccaagg agauagccug     2160

caugagcaca uugccaacuu ggcaggcucu ccugcgauca aaaaggggau acuccagaca     2220

gugaaaguag uugacgaacu ggugaagguc augggacguc acaagccaga gaauauugug     2280

aucgaaaugg cuagagagaa ccagacuacc cagaaagguc aaaagaacuc aagggagcgc     2340

augaaacgga uugaagaggg caucaaggaa cuagggaguc agauacugaa agagcauccc     2400

gucgagaaua cacagcucca gaaugaaaag cuguaccuuu auuacuugca aaacggaaga     2460

gauauguaug uggaccagga gcuggauauc aauagguuaa gcgacuacga uguugaccac     2520

auugugccgc aguccuucau caaagaugac ucuauugaua acaagguacu cacccgaucg     2580

gacaagaauc gcggcaaaag cgacaacguc ccuuccgaag aggugguuaa gaaaaugaag     2640

aauuauuggc ggcaacugcu uaacgccaaa cugaucacuc agagaaaguu ugauaaucua     2700

acgaaggcag aaaggggugg gcucucagag cuggacaaag ccggcuucau aaagcgucag     2760

uugguggaga cacgccagau uaccaaacau gucgcucaaa uccuggauag ucggaugaac     2820

acuaaguacg acgaaaacga uaaauuaauc agagagguga agguaauuac ccucaagucu     2880

aaacugguga gcgacuuuag gaaggauuuc caguucuaua aaguucgaga aaucaauaac     2940

uaccaccacg cccaugacgc auaccuuaau gcugucgugg gaacagcccu gauuaagaaa     3000

uaucccaagu uggaguccga auuuguguac ggcgauuaua aggucuacga cguucggaaa     3060

augauagcga agucagagca ggagaucggg aaagccaccg cuaaguauuu cuuuuacagc     3120

aacauuauga auuucuuuaa aacugaaauc acacucgcaa acggagagau ucgcaagaga     3180

ccacugaucg aaacgaaugg ugagaccggc gaaauagugu gggauaaggg gagggacuuc     3240

gccacugugc ggaaaguacu aucuaugccu caggucaaca ucgugaagaa aacagagguu     3300

caaaccggag gcuuuuccaa ggagaguauu cugcccaaac gaaauagcga uaaacuuauc     3360

gcuagaaaga aggacuggga cccaaagaaa uauggugggu ucgauucucc cacaguggcc     3420

uacuccgucc ucgugguugc aaagguggaa aaaggcaaga guaaaaagcu gaagucagua     3480

aaagaguugc ugggauuaac uauuauggaa cgcagcucgu uugagaagaa cccuaucgac     3540

uuccucgagg cgaaaggcua caaggaaguc aaaaaggauc ugauaauuaa acuuccaaaa     3600

uauucccugu uugaguugga aaaugggagg aagcguaugc ucgccucugc uggagagcug     3660

cagaaaggca acgaacuagc ccugccgagc aaguacguga auuuccuuua ucucgcauca     3720

cacuacgaga aacugaaggg uagucccgag gacaacgaac agaagcaguu guucguggag     3780

caacauaaac acuauuuaga ugaaaucauc gagcagauuu ccgaauuuuc uaagcggguc     3840

auacuggcug acgccaaucu cgauaaaguu cugagcgcau acaacaagca uagagacaaa     3900

ccuaucaggg agcaggccga gaacauuauc caccuuuuca cccugacgaa uuugggggcu     3960

ccagcggccu uuaaguauuu cgauaccaca auugaccgca aacgauacac uuccaccaag     4020

gaagugcuag augcaacacu gauccaccaa ucaauaacug gacucuacga gaccagaauc     4080

gaccugagcc agcuuggcgg ugauuga                                         4107


<210>  3119
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3119
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa cagucaacga gaaauccucu auaguuaagu ugggugugag gguaaauccc      120

uuaacugugg augaacagaa aaacuucgag gcaggcaagu caaucccuac gaaugccggg      180

cggacccuga aacgcggaau gcgacguaac cuacaaagau acaagcucag gcgggacuau      240

cugauucagu gccuuaagga auacggcugg aucacaagug auacucuguu guaugagugu      300

gguaaucgca ccacauuuga gaccuacaga cucagggcga aagcugucga cgaagagauu      360

gcccuggagg aacuggggcg ggugcucuua augaucaaca agaaacgcgg auauaagagc      420

aaucgaaaag caaaguccuc ugaugagggc cagcugauag acggcauggc caucgcuaug      480

gauauguacc acaacgggac uacaccagga gaauauguuu acucgcuuuu gucaaagggu      540

aaaaaguaca uuccgguguu cuauagaagc gaccugcuag augaguuuga caggaucugg      600

gauuaccagu gcgcauauua ccccgacauu cucaccaguc aauucaaaga acugauccgg      660

ggcaaguccu cucaggucac gagcaaacuu uuuaagcagc aguauaaucu guucacugcc      720

gauaacaaag agaaggacaa gcguuugcag gcuuacuguc gcagagccga cgcgcucaaa      780

aagaggcugc cuguggaaga gguagcagcc gcuuuaugcg auguuaaucg ggacgugaac      840

gccuccucag gguaucuggg aaccauaagu gaucgaagca aaaagcucuu uuucaauaaa      900

caaacagucg gccaguaccu uaugcuggag uuggacaagg auucucauuu uuccgugaag      960

aacgaagugu ucuaccgcca ggacuaucua aacgaguuug aagcaauuug ggagaaacaa     1020

gcugaauacc acccagagcu gacucccgau agaaagaaaa uccucaggga cgucguuauu     1080

uucuaucagc ggccucugaa gucacagaaa gaucuugugg ccuguugcga gcuggaacgc     1140

agaaggaucg agguaaugaa ggacgguaag gugcguagcg ucgaaaucgg gccaaaagug     1200

ugucccaaga guucuccuuu guuucaagau uucaaaauuu ggcauguucu caauaaccug     1260

aaggugcggg acaaagcgac cggagacgag cgauucuuag aacuggauga gaaggagaua     1320

cuauuuuccg aacucgccau caagagcaaa cugucgaaga cagaggcacu uaaauugcug     1380

uaccagaagc caaaaggccu cgaccugaau uaugauucug ucgaaggcaa caccacgcag     1440

gcugcccuuu ucgaggcuua ccagacugug auugccauga cagggcacgg agaguaugua     1500

uuuuccaaga ugucagcaag ugaaguuaug agcguggucg aggguguguu cggcgggcug     1560

ggauuuaaua ccuccgucuu gagauucgac ucucuccugc aaggcaaaga auuagagaag     1620

caggauagcu acaggcugug gcaucuacuc uacucauaug aaggugacaa cagugugacu     1680

gggacagaug gccugguugc caagguggcu ucccuuuugc gcugcgacga ggaguacgcg     1740

aaaguagucg caggagugac cuuucagccg gauuauggca gccugucugc caaggcuauc     1800

cggagaauac ucccccacau gagggccggg aaugaauacu cacuggcaug ugaguaugcc     1860

ggauacaaac acagucgcag gagcuuaacg aaggaggaac uggaacagaa agacuauaag     1920

gaucaucuug aguugcuccc ucggaacucc cugcguaauc caguugugga gaagauucua     1980

aaccaaaugg ucaauguggu aaacgcugug guugacgccu acgguaaacc cgaugaaauc     2040

agaaucgaga uggcaaggga acugaagaaa ucugcgaagg agcgcgaaga gaugaccgcu     2100

gccauuaaua aagcaacauc ggagcacgaa cggaucaagg agauucucaa caaggaauuu     2160

ggcauacagc augucagcca caaugacauc gugagauaca aacuguauug cgagcuuaag     2220

aacaacgggu acaaaacuuu guauuccgac accuacauuc cucaggagaa gcuguuuuca     2280

aaagaagugg auaucgagca caucuuacca caggcccgac ucuucgacga uaguuuuucu     2340

aauaagacac uggaauaucg aagcgucaac auugagaagu ccaaucaaac ugcuugggac     2400

uacguugccg caaaaugggg agaagagggc ucacuucgcu auaagaaacg gguggaugac     2460

cuguuggcug ccggugugau aggcaagagc aaacaugaua agcuccugau gaaacaggag     2520

gacaucccca ccgauuucau uaacagagac cuaagggaua cacaguacau cgcgcguaag     2580

gcccgcgaaa uucuggagga ccucguaccg auggucgugu cuaccacugg ggcuguuacg     2640

gaucggcuga gagaagacug gcagcuugug gacgucaugc aagaguugaa uugggauaag     2700

uacgcaauga aaggacugac agaagagugg accgacaagg auggcaggcg aaucucccgg     2760

auaaaagagu ggacuaagcg caacgaccac agacaucacg ccauggaugc uuuaaccauc     2820

gccuuuacaa aacauaguuu cauucaguau cucaauaacc ugaaugcaag gagcgacaag     2880

ucugggucca ucuacgcgau ugaacagaag gagcuuuauc gggauaacca cggaaaacug     2940

cgauucauca ugccuuuuga agguuucaga ucagaggcca agcgccaacu agaaaauaua     3000

cucgugagua uuaaagcuaa gaacaaaguu gugaccagga auguaaacaa ugcccgucgg     3060

ggcagcggga gacaggcaaa gguccagcug acucccaggg gacaguugca caacgagaca     3120

aucuacggcu cgauucaucg cuaugugacg aagcuggaga aagugggugg gaacuuugac     3180

ggcgaacaaa ucgcuaaggu cgccaauuua cgauacagag aggcacucca ggcccggcac     3240

gaggcuuucg gaggcgaugc gaaaaaggcc uuuaccggga aaaacgcacu ggacaagaau     3300

ccacuuuauc ugaaggauac uaggacagaa guuccuuuga aagugaagac cguauccaca     3360

gagacucucu acaccauacg caaagcugug gcccccgacc ugaacgucga caaggugauc     3420

gaugccaaag uucguaaggu gcuagaagag agacugcagg aauacggagg cgacccaaag     3480

aaggcuuucg ucaaucuuga ugagaacccc auuuaucuca auaaagcaaa agguaucacg     3540

auuaagcggg uggccaucac cgggguuaac aaugcgauag cucugcauga caaaaaggau     3600

gugggaggcc acgucauguu ggacgccucu gguaggaaac agccuguaga uuuugugaac     3660

acaggcaaua accaccaugu ugcaauuuac cgcgacgccg aagggagccu gcaagaggug     3720

gucgugucau ucuaugaggc uguagcacga gugaguuuag gacucccagu caucgauaga     3780

gaauaccgga aggaggacgg cuggcaguuu cuguucacua ugaagcagaa ugaauauuuu     3840

guuuucccga accccgagac cggguuuaau ccucaggaag uggaucuuac agacgagacu     3900

aacuacgccc ugaucucccc aaacuuguuc agggugcaaa aauucaccau uaaggauuau     3960

uucuuccgcc accaucucga gacgacaguc gaagacagaa aagagcugaa ggacaugacc     4020

uggaaaaggu guggacuagg cgguaucauu gggauaguga agguucgggu aaaucacauc     4080

ggacguauug ugucugucgg cgaauacuga                                      4110


<210>  3120
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3120
auggccaaga ccuacaacau cggccuggac auugggacaa auagcauagg augggcugug       60

gucgaugacc agaaccaccu cauguccauc aaagguaagu auggcuacgg gguuagacuu      120

uaugaugagg gaaaaacugc agaagagagg cggucuuucc gcacgacccg acguagacug      180

aagaggcgga aauggcgcuu gucauuacug agagaaauuu uugagcccca aaucacaccu      240

cuagacaaua cuuucuuucu caggcagaag cagaguagcc uguccucuaa ggauaaccgg      300

aaacuuuacc cagaaauguc gcuguucaau gaccgcaccg auacagacuu uuauacccag      360

uacccgacua uuuaucauuu gucacgagau cucaugacaa agaacaaaca auucgacauc      420

agagagauau accuggccau guaccacauc gugaaguaua ggggccauuu ucugaaugag      480

agccccguaa guuccuucaa aucuagcgaa auuaagcucg cggagaacuu ugaaaccuua      540

aaucagaucu ucaacgagcg guuuaaugag uccauguucc agcuggcugu gaacgauuuu      600

gacgaucuua aggccuugcu gcuaaaugac cagcaaucag caaguaaccg ucagaaaaag      660

gcccucggua ugauuuacgu cccuaaugaa aacaaaagcc uggagaagca gaacaaagcu      720

aucgcaacgg aacuucugaa ggccauauug gggcucaaag cuaaguucaa ugugcugacu      780

ggaauugagg uugacgaugc caagccaugg accuuaucuu ucaacuccga aucauuugac      840

gaggauaucg acaaagcggc aacaaauauu gaugacgaua gccgcgagau caucgccauu      900

cugcagggcc ucuauagugg cauacuucug gcugggauca uucccgaaaa ccaaucuuug      960

uccgaggcca ugguggaaaa guacgaccag cacgcagcuc aucuaaaaca gcuuaagagc     1020

aucuauccuc uccugucgau aucugauaaa aaggccauuc aacaggcgua caauuauuac     1080

aucgacggac aggauaacgc caaggcauac uccagagagg acuucuaugc ugccacuacc     1140

aaagcucuua agucauacca ggacaaugaa acaaucgccg agauucugaa auugaucgag     1200

cucgaacaau uuaugccaaa gcagaggacc aaagauaacg gugcaauucc ccaccagcug     1260

caccagcaag aguuagaacg gauaaucgag aaucagcaga aguauuaccc uuggcuggcc     1320

gaacuaaacc caaaugagag ucgacgcaag auugcuaaau auaagcucga cgagcugguc     1380

gcguucagag ugccguacua uguaggcccc cuuaucacga gcgaacagca acagcagucc     1440

ucuaacaaaa aguuugcaug gaugaucagg aaagccgagg ggaagauuac uccuuggaau     1500

uucgaugaaa agguugaccg gcaggcuagc gccaacgagu uuauaaaacg caugacaacc     1560

acugauacau acuugcuggc agaggacgug cucccaaagc aaucacuggu cuaccagaga     1620

uucgaagugc uuaaugagcu gaacggaauc aaaauugaug acgugcccau caccgucgaa     1680

cagaagcaaa ggauuuuuaa cgauuuguuc augaugcagg ccaguguuuc cgugaaaaau     1740

auccaggacu aucucguaag caagggcuac uuugcuucuc guccugagau aacgggucug     1800

ucagaugaaa acaaagugau cagucgguua agcaccuauc augaccugaa ggccauucua     1860

gggggaaucg ucgaugaggc aaauaagcag gcggaccucg agaaaugcgu ggaauggucc     1920

acaauuuucg aggacggcaa gaucuuuguu gaaaaacuga acgagauaga auggcuuacu     1980

gaugagcaac gaaagagauu gucuucgaaa agguaccgcg gcugggggcg gcugagcaau     2040

aagcuccuga accagauugu gaaugagaac ggacagagaa ucauggacuu acugugggau     2100

accacagaca auuucaugag gauuguccac uccgaagagu ucgauaaacu uaucucagcu     2160

gccaaccaga cuuugcucag uaagaaugac gugcaagaug uaauaaacga ccuguauacc     2220

ucuccacaga auaagaaagc acuacgccag auccugcucg uugugagcga uauucagaag     2280

gccaugaaaa accaagcucc cuccaagauc cugauugaau uugcccgaga ggacgaagca     2340

aauccgcggc uuucagucca gcguaagaga cagguggagc aagucuacca gaacaucagc     2400

aaagauauau ugucugacuc cgaugugaag gcugagcuga aagacuuaac aaacaccgcc     2460

cucgcgaaua cuaggcuguu ccuuuauuuu augcagggug gccaugacau guacacgggg     2520

gaaacacuga acauugaucg cuugagugag uacgacaucg aucacauccu cccucaggcc     2580

uucauuaagg acaauagccu ggauaaccgg guucuagugu cugcuaaaau gaauagaucc     2640

aagucagacc aaguaccccu gaacgauuau accgaagaga gguuuggaag uaagaugcga     2700

guccaguggg agcagaugcu ccgcgcaggc cugaucacua aacggaagua cgacaaucuu     2760

accuugaacc cagauagaau uagcaaauau gccaugaagg guuucauaaa uaggcagcug     2820

guggaaacac gucaaguuau caaauuagug accaaccucc ugauggacca cuacgaggau     2880

gacgaagucc agcuuauuac ugugaagucg gggcugaucc aucagaugcg cgagauguuu     2940

gacuucccua aaaaucggaa ccuaaauaac caccaucacg cuuuugaugc cuaucucaca     3000

gcauucguag gcauuuaccu guugaagaga uaucccaagc ugacgccauu uuucguguac     3060

ggauccuacc agaaaaauaa caccaacgac aaguggagga auuuuaacuu cuuaaaugau     3120

cucaaaaagg aagagguuau cgacucugau agccaugagg ucauauggaa caaagcgcaa     3180

ggccuggugg aacuugacaa gaucuaucag uucaagaaaa uucuggugac ucgagagguc     3240

cacgaaaaua caggggccuu guacaaccag acccucuauu cagcuaagga ugacaaagcc     3300

aguggacagg guggcaagca acugaucccc gcaaaacagg aucggccuac agcucuauac     3360

gggggauauu ccggcaagac uguugccuac auguguauug ugcgcaucaa gucuaaaaag     3420

gaggacaccu auaaagugug cgguguaaag acgagcuggg ucgcagaacu ggagagacuu     3480

uccgauucaa augagcagcu cgccagccug aaagcuaagu ugaugccaaa guuuaccaaa     3540

aacaagaaac aaaaggggaa aacaauacag gugacugaag acuucgagau uaucguuccg     3600

agggugcuga ucaaucagcg guuuuacgau aacggccagg aaauuaccuu aggaagugcg     3660

acauacaagc acaaugagca agaacucaug cugaacaaga aagccgucaa gcugcugaau     3720

ggccaguugu cucucgugga ggcagagacu cuggcucccc agguuuauga cgaaauacua     3780

aaacagguga ugaaguacuu cccucuguau gacaccaacc aauuucgaac gaaacuuaag     3840

auggggaaag agcaguucau caagcuccca uggaagaaua aaugggaugg aaacaagcug     3900

auucauguag gucagcagac agucuuggac agaaucuuaa uuggccugca cgccaaugcc     3960

gcuguguccg aucucagcac ccugaaagug acuacacccu uugggaaacu ucaacagccu     4020

ucgggaaucg cacugucauu gaacaccaag auaaucuacc agucuccaac uggccuauuc     4080

gaacgcaggg ucgcccugcg uggucucuga                                      4110


<210>  3121
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3121
auggacaagc ccuacaacau cggccuggau auugggagcc acuccauagg auggaccgug       60

gucgacgaua auucuaaacu cguuagaauc aaggguaaac uuggcuaugg ggugaggcug      120

uucgacgagg gaaagacagc cgaagaacgg cgccaguuuc gaacuacgcg uagaagguug      180

aaacggcgca aguggagauu aaggcugcua cgggaaauuu ucgagccuca uaucgcucca      240

cucgaugaaa ccuuuuuccu gcgccaaaag cagucaaacc uuguaccgac acagaaaaag      300

cugccccagu ugagucucuu uaaugaccga agcgaugcag aguucuacca caaauauccu      360

acuauuuacc aucugagaaa cgcccugaug accgagaaga ggcaauuuga ccuccgggaa      420

aucuauuuag cgauacacca uaucgugaaa uaccguggcc acuuccugaa ugcugcccca      480

gcagccuccu uuaagucugg uaaguucgau cuugagaaca aauuugacca guugaaucug      540

cuauucgcuu cggcauauuc agaucucgac uuuacacugc gcaccgauaa cgucgaccag      600

gugaaggcca uucuucugaa ugaagacuug agcgcuagua cuagacagag gcaagcccuc      660

ccccugaucu accagacauc cgcggaucag aaagcagcca agaaauuaac caagucucug      720

gcuacggagc uccuuaaagc cauucugggg uugaaggcaa aguucgacgu ucuaacugga      780

aucgagaccg augacacaaa ggcuuggcgg cugacuuuca acgccgaaga uuuugacgau      840

aagcucaaug agcugaacgu gaccgacaau gaagcggcca cacagauaau ugagauccuu      900

cgagaacugu acagcuccau uuugcucgca gcuaucgucc cugagaacua uacccuguca      960

caagccauga uggcuaaaua caaugcccac aagcagcauu uaagucugcu acgccaggug     1020

aaaacgacuc ucccagcaaa gaagcaaaaa gagcugcagg ccgcuuauga ugacuacauc     1080

gaucagcacc uucccuugga caaguucuau gcgaaacuga caaagguacu cccuaaagaa     1140

aaggacaccg auaacccaca gauuauacaa auccugaacc uuauugaccu ggaucaguuu     1200

uugccgaagc agagaacuaa agacaauggc gcaauccccc aucagcucca ccaacaggag     1260

cuggaucaga uaauugacaa ccagaaggcc uacuauccuu gguuagcuca acugaaucca     1320

aacgaaaaga ggcagaagau cgccaaauac aagcuagaug agcucguugc auuccgggug     1380

cccuacuaug ucggcccucu gaucgaagcc aagacacagc agaaaaccag cggggcuaau     1440

uuugccugga ugauucgcaa ggagccagga acgaccacac ccaucacucc guggaacuuc     1500

gacgagaaag ugaauagaca agcaucugcg aacgaauuua uuaagaggau gaccacaacu     1560

gauaccuacc uuuuggguga ggacgugcug ccuaaacacu cccuccugua ucagcguuuc     1620

gaagucuuaa augagcugaa cggcauaaag aucgaucaga aacggguuac agugcgacaa     1680

aagcaggcua uuuuuaauga ccuuuucuug aagcagccca acguaaccgu gaaaaagcuc     1740

cagcaauacc ugcaggccaa aggggaauau gcaucagccc caaucaucac uggacuaagc     1800

gauccuaaga aauuuaauag ucagcugucu acguaccaug aguauaagaa gauuuucggc     1860

ggucucauag acgaccccga uaaacaggcu gacuuugaga agugcaucga augguccaca     1920

auuuucgagg auagccaaau ccugaaagac aaacuuaaag aaauuaacug guugacccca     1980

gagcagagac acaaggucgc caauaagagg uaccaggggu ggggacgccu gucgaaaaag     2040

uuacucacuc agcuggugga uuacaacggc caacggauca uagacauccu uuggaacacc     2100

acagauaauu ucaugcagau uguucauucu gaggcauuug acaaagcuau cauuaaggaa     2160

aaccaggccc uguugaccca auauaauccc caggcgguga ucgaugaccu cuacacuucc     2220

ccucagaaca aaaaggcccu gagacagaua cuacuggucg ugaaugauau ucaaaaggcu     2280

cucaaaggcc aggcaccauc aaggauccug auugaguucg cccgcgaagg gcaggagaac     2340

ccgcgacuua guacacagcg gaagcaacag guagaaaaug cuuauaaaga ggccagcgac     2400

gcauugcugu ccucugcgga gguucgucag gaauuaaagg gacucgauaa caaagcccug     2460

gcuaauacga gacuuuuucu guacuucaug caggguggca gggacaucua uaccgggaaa     2520

agccuaaaca uagacgaucu cucaaaauac gacaucgauc acauucugcc ccaagccuuu     2580

aucaaggaca auaguuugga uaaccgcgug cuggucuccg caaagaugaa ucggagcaaa     2640

ucugaccagg ugccuuuaaa cgauuucgcu gagauuagau auggaaagca gaugaggcag     2700

caaugggaga aaaugcuccg agccggccug aucacucgca agaaauacca uaaucuuaca     2760

cugaacccag agucaauaaa caaguacacc gcaaaggguu uuauuaaucg gcaguugguc     2820

gaaacaagac aggugacuaa acucgccacc aaccugcuag uugaccaaua ugcugaggau     2880

gacgugcagc ugaucacggu aaagaguaau cuuacccacc agaugaggga ggaguucgau     2940

uuucccaaaa accguaaugu caacaaucac caucacgcgu ucgacgccua ccucacagca     3000

uuuguggggc uguauuugcu gaagcgcuac ccuaaauuag cuccauauuu cguuuacggc     3060

gacuaucaga agggagccag ccaaaacucc aaguggcgga auuuuaacuu ccucggcaau     3120

cugcauagag aaaaaguggu cgauaagaac acuggggaaa ucauuuggca gagggacgcc     3180

ggacuuggug agcugaaucg aaucuacaac uucaaaaaga uuuuggugac ccgggcucuc     3240

cgcguaaaua gaggccccau guuuaaacag acaauauaca aggcagaaga ggauaagucu     3300

ucggggaggg gaagcaaaca gcugaucccg acuaagaaaa accggccuac cgagcuauau     3360

ggcgguuaca cgaacaagga aacagccuau cugugucuug ugaaagagcc acacaagaag     3420

ggggacauuu accgaaucug cuccguucuc aauauguggc ugaccgaauu ggauagaauu     3480

gcggacccca ucgcuaaagc ccaaaaguua acugagcugc ucacaccuca guucaccacu     3540

accaaaaaga aacagggcca gaagauaccc gucgugaaac cauuuaaggu ggucaucccu     3600

aagauucuga ucaaccaacg cuucuuuuca aggggacagg agcuuacagu ugcaaguccc     3660

auguauaugc acaaugaaca ggagcuguuc uugaacaaaa agacgauuaa acuacugaau     3720

ggccaacucg ccaagaacga agcucaugag cuggcaccaa aaaucuacca gcagauacuu     3780

gaucagguga augacuauuu uccguuauac gauauguaca aguuccguga cacccugaac     3840

gccucuaagg aaauuuuugu gaaucucccu uggcaagauc agcggcagga caaaaagauc     3900

agccaguccg ggcaaaaauc aaucuugaac aagauucuga uaggacucca cgcgaaugcu     3960

gccagaauca aacugacuga gcuuaacuuc aagagcgauu uuggucuguu gucucagagg     4020

uccggcauug cauucagucc cgacgaccag aucauuuauc agagcccaac agggcuauuc     4080

gagcgccgag uaacccugaa ggaucucuga                                      4110


<210>  3122
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3122
augaagguga acaauuacca caucggccug gacauuggga ccagcuccau aggauggguc       60

gccaugggug aggauggcaa accccucaga guuaagggga aaacagcuau cggagcaagg      120

cuuuuccggg aaggcaaccc ugccgcggac cgccgaaugu uucguacuac gagaaggcgg      180

cugaaucgca gaaaguggag guugaaauua cuggaugaga uuuucgaccc auauaucacc      240

ccgguggauu cuacauuuuu cgcucggcua aagcagucaa acuggagucc caaggacagc      300

cgcaaagccu uuaaggauuc cacucucuuc ccugaccuga ccgauaaaca auaccaucuu      360

gacuauccaa caauuuacca cuuacgacau gcauugauga cccaggauaa gcaguuugac      420

aucagaaugg uauaucucgc ccugcaccau auagugaaau acagggguaa uuuccugaac      480

ucuacucccg ucgauucguu uaaggcuuca aaggugaauu ucgacgacca guuuaaaaag      540

cucaacgaau uauauacagc aaucaauccu gaggaaucau uccaaauuaa cuuugccaau      600

agugagaaaa ucgggcagca guuccuggau ccauccauuc ggaaguucga caaaaagaaa      660

cagaucucua agauaguucc cgugaccaug gaugacaagg cugucgauaa acuuaacgga      720

aagauugcca gcgagaucgu gaaugcgauu uugggcuaca aauccaagcu ggacguagca      780

guucaaugcg auccugaaga cucaaaacca ugggccguga aguuugauga cgaggauauc      840

gacgcuaagc uacagaaaau ccucccggaa auggacgaga accagcagag uauuauaggc      900

aucauucaag aucuguacag ccaggucacg cuuaaucaga ucgugcccaa cgggaugucu      960

cuguccgagu caaugauaaa gaaauauaac gaccaccacg aucauuugaa gcucuacaaa     1020

aagcugauug gacaaaucag cgacaguaag aaaaagacug cccugaaaaa ggcauaugau     1080

caguacgugg guaaugacgg caaagucauc gaacaggaug acuucuauuc uuccguuaag     1140

aagaaccugg augacagcga gcucucgaaa cagauuaugg aucuuaucga cgcugauaag     1200

uuuaugccua aacaacguac cucucagaau ggggugauuc cacaccagcu gcaucagcgc     1260

gaauuggacg agauaaucga acaccaaucc aaguacuauc ccuggcuagc cgagucaaac     1320

ccuaauaaac acgaccugca ucucgcgaag uacaaaauug aggaacuggu agccuucaga     1380

aucccauacu augugggacc caugaucaca ccggaggauc agaagaagag uggcgcagcu     1440

gucuuuagcu ggaugaaaag gaaggaaccu ggucagauua cucccuggaa cuucgaccag     1500

aaaguggauc ggauggaguc cgccaauaag uuuauaaaac gaaugaccac aaaggacacc     1560

uaccuuaucg gggaagaugu ucugccagac gagucuuugc ucuaugagaa guucaaagug     1620

cugaacgaau uaaauauggu ccgcgugaac ggagauagcc ugaagguagc ugacaaacaa     1680

gccauuuacc aggaucuauu ugagaauuau aagcacaucu caguuaaaaa gaugcagaac     1740

uacauuaagg acaaaacggg ccucccuaau aaccccgaua ucaguggccu guccgaccca     1800

gaucauuuca auaacagccu ugggacuuau aacgacuuua agaaauuguu cggagacaag     1860

guggaugacc ccgaucugca ggacgauuuu gaaaagauag ucgagugguc uacaguguuc     1920

gaagacaaga agauccucag agcaaaacug aaugagauuu cauggcuuag ugauaagcaa     1980

aaagacgauc uggagagcuc cagguaccag cggugggguc gcuugucuaa gaaacuccug     2040

accggcaucg ucaacgacca gggggaaaga auuaucgaug aguuauggaa uacuaacaag     2100

aauuucaugc aaauacaguc ggacgacgau uuugccaaaa ggauucacga agcuaacgcg     2160

gaccagauga aggcaacaga uguggaggac guucuggccg augcuuacac cagcccucag     2220

aauaagaaag ccauccguca agugguaaag gucguggacg auauucagcg ggcaauggga     2280

ggcguugccc caaaauucau cuccauagag uuuacgcgau cagaagacag aaacccgagg     2340

cgcaccauca gucgggagag acagcuagaa gauacacuca aggacacugc uaaaacccug     2400

gugaagucua ugaaucccga uauucuuagc gaguuggaca acgccuccaa gagcaaaaag     2460

ggucugacag auaggcucua ucuguacuuc acucaguuag ggcgcgacau guauaccggc     2520

aaaccuauca auauugacga aaucagcaac uacgauauag accauauucu gccacaagca     2580

uuuaucaagg augacucucu uuccaaucga gucuugguga guaaagcggu aaacaaugga     2640

aagagcgcua acgugccccu caagcuguuc ggcguuaaaa uggcccacuu uuggaagcag     2700

cuagcagagg ccgggcugau caacaaacgg aagcucaaaa aucugcagac aaacccugau     2760

accauuucua aguucgcuau gcacggauuu auccguagac agcuugucga gacuucccaa     2820

gugauuaagu ugguggccaa uauacugggu gacaaauaua ggaacgauga cacgaagauc     2880

auugaaauca cagcacgcau gaaucaucag augcgggaug aguucgcuuu uauuaaaaac     2940

agagaaauca augacuacca ccaugccuuc gaugcguauu uaaccgccuu ucucggcagg     3000

uaccuguacc accgauauau aaaacuucgc ccauacuucg ucuaugggga cuucaaaaag     3060

uuucgggagg auaagguuac uaugagaaac uucaauuuuc ugcacgacuu gaccgacgau     3120

acacaggcua ggaucgcaga caccgaaacu ggagagguga uuugggaucg ugagacauca     3180

aucaaacaac uccgcgacgu guaccauuau aaguucaugc ugauuaguca ggaaguauac     3240

acgcuacggg gcgccauguu uaaccagacc aucuaucccg cugaugaggc cgguagaaag     3300

aagcugauac cgacuaaagc agacaaggcg gucgaugugu acgggggcua cagcggaucg     3360

aaugacgccu auauggcuau uguuaggauc cacgauaaaa agggcgacaa guacaaagug     3420

gucggggugc cuaugcgagc ccucgaucgg cugaacgcag cuaagaaugu uuccgacgcc     3480

aaauaugacc aggugcuuaa ggauguauug acaccacaau ucaccaaauc uaaaaagagc     3540

cgcaaaacag gagaaaucuc acagacuguc gaggacuuug aaguggugcu ggguaagguc     3600

auguacagac aguuaguuau ugauggcgac aaaaaguuca ugcucgggag uuccaccuau     3660

caguacaacg caaaacaacu ggugcuuucu gaugagagcg uaaagacgcu ggccuccaag     3720

ggaaaacuag acgaggcuca ggaaucagcg gccuaugaua auguguacga cgagauacuc     3780

gauaaggucg acagguacuu ugcacuguau gaucagcgga acuaccgaac caacuugcau     3840

gaaggcgagc agaaauucau cggucugccc uuacacgaca cauuugaugg gagcaagaaa     3900

guggcuaaug gcaagagaga gacuauuagu gacauccuca acggacugca ugccaaugcc     3960

accaugacaa accuuaaggu ucugggcauu ucuuccccuu ucgggauguu ccaaagccac     4020

ggacccauca auuugaaccc agaaacuaua auccucuguc agucgccuac cggccuguuu     4080

gagcgcagga uuauccuacg ugaccuguga                                      4110


<210>  3123
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3123
augaagaaaa gaaacuacaa uaucggccug gacauuggga ccagcuccau aggauggaca       60

aucguggaug acaacgccca gauuaugagg gcuaagggua aaaauggcua ugggguccgg      120

aucuucgcag agggaaagac ugccgcggau cgccgaggcu uucguacgac cagaaggcgg      180

cucaaacgca gaaaguggag gcuucggcug uugcaagagu ucuuugaacc cuacauuuua      240

ccuaucgacg aaaacuucuu uaugcgccag aaggagucua aucugguucc acaggauccg      300

aacaaacacu ucaaggguuc aauacuauuu aaugaccgaa cagaucagga guuccaugaa      360

caauauccca cuaucuacca ccucagauau gcucugauga ccgagcauag gcaguuugac      420

gugcgggaaa uuuacugggc cauccaccau auuguaaaau aucgugggca cuuccuuaac      480

aguacacugg caagcaaguu uaaaggaggc gaguugcgcc ucgccgauaa guucgaaucc      540

cugaauaagc uguuucucag agugcaggcu gagaccggcu ucgaguuauc ugaagacaac      600

gucgauaaaa ucaagacugu gcugcuugac aaugggaaau cgagguugga ucggcagcga      660

gagguucuga agauagugua ccuaacauca gaagacaaag acaccgagaa gaaacgcaag      720

gcagucgcca cggaguucau uaaggcugug cucggacuga aaacuaaacu uuuuaucgua      780

cuggccaaaa acccuaccgc ggaaacagau uggaaguuca gcuuugacgc agaggauauu      840

gacgauaaau uggacaguau cuccgaacaa cucacugaug acgaucacga gaucguugaa      900

cugauuucug agauacauag caucauuacc uuaacaggug uggucccaga aggcaugggg      960

cuguccgagu caaugaucgc caaguacgac cagcaccagc ucgaucuuaa gaaacugaag     1020

aguuugauga gagaccuaga cgugaaacag gcuaggaagc ugcaagaugc cuaugacgag     1080

uacauagaug gacucaaacc cagcgaagag gcauucuauu cugcuguguc cucagucauu     1140

cagaagaugg acgauccucg ggccaagaaa aucaccgcgg aaaucgagcu ggaagaguuu     1200

augccaaagc agcgcacgaa agccaauggc caaauucccc aucagcuuca ccagcaggag     1260

cuggaccaaa ucauugaaaa ccagaaggag uacuauccuu gguuggcaga agagaaucca     1320

aacaaaagag agcacaacgc ugccccgcau aagcucgaug gucugguuac uuucagggug     1380

cccuacuaug uagggccuuu aauagaagcu agcgugcagc agaagacaag uaauucuaac     1440

uuugccugga uggagcguaa agucaagaau gacaccuccc ggaucacucc auggaacuuc     1500

gaagagaaag uggaucgaaa ugcaagcgcc gaaaacuuua uuaagagaau gacaaccaaa     1560

gacacguacc ugcuaaauga ggauguucuc ccccaacagu cgcugcuuua ccagagguau     1620

gagguguuga acgaacugaa uaacguccgc gugaaugaca agaaacucga gaacaauugc     1680

aagcaggaug uauacaagga ccuguucaaa aacaauacca caguuucuau caagaaauuu     1740

aaggacuauc uuguggcuaa cugugaauac gcggauaacc cuaaaaucga gggacuggca     1800

gacgaaucca uuuucuuguc aagucucagc acugagaaua aguuuaagga uauauucggc     1860

gcccaaaucg acgauccaaa cuauuucgag gacuuugaac acauuaucga gugggcuacc     1920

auuuucgaag auaauaaaau ccugcgggcc aaguuagacg agauauccug gcugaacgaa     1980

ucucagaaaa aggagcuagc aaaacucaga augaggggcu gggggcgccu gagccgaaaa     2040

cuuaucgccg agauuacaga ugaaaaugga ucacggauca uugacuugcu gugggagcau     2100

auggauaacu uuauggucgu ggucaaucag gacguguuca aggaaaagau cguugcugag     2160

aacaaacagg uguuuaagga gcucaauccc cuggauauaa uuaacgaaca auucaccagu     2220

ccgcagaaua agaaagccuu acgucagauc cugcuuguag ucgcagacau ucaaaaggcg     2280

augaacggug ugacuccuuc caauaucuuu auagaguucg cuagagaaga cgagaggaac     2340

ccccgccgga gcauccagag auugaggcag guugaaaaua uguaccgaga uauuucugac     2400

acagugcucg ccgauccaga gguccgcaac gagcugucaa acaucaagaa uaccgaacua     2460

gcacgggaca gaguguuucu guauuuccuc cagggcggga gggauguaua cacuggagag     2520

ccucugaaca uugacaaacu uagugaauac gauaucgacc acauauugcc ccaagccuuu     2580

auuaaggaua auagccugga caaccgugug uuaguuuccg cuucucucaa ucgcucgaaa     2640

agcgauuccg ucccacugga cgacuucacg ucagagaugu uuggccggaa gauggaaccc     2700

cuuugggagc agaugcagag agccggucug aucaguaaaa agaaauuggc aaaccucaag     2760

uucaauccug agaacaucaa uaaguauaca aggaacgggu ucauuaaucg acagcuggug     2820

gaaacccggc aagugaucaa acuagcugcc aacauucuga aggaucauua cggcgaggaa     2880

acugagauag ucaauguuaa agcgggacuc acccaccagu uucgcaagga cuucgauuuu     2940

ccaaagaaca gaaaugugaa caacuaucac caugccuucg acgcuuaccu gacagcacuu     3000

gugggcaccu auuugcugaa gaaguacccg aaauuagaau cuuauuuugu auacgggaag     3060

uucaaaaagg gagaugguau cacuaaucuc aaaaacuuua auuuccugca cggcuuuaac     3120

aagcccggga agauuaucaa uaaagagaca ggagagauuc uuuucgacag ggauaagcag     3180

cugggcuaca ugaacagcau cuaugaccua cggaaaguca uagugacgca ugaacucucc     3240

acccgacacg gugccaugua cgaucagacu cuguauaagg cuucagacga uaaagacagc     3300

gggagaggcg gaaagcaauu gauccgcaag aaagauaauc ugccuacaga gauuuacggc     3360

ggguauaccg gaucuacaga cgccuuuaug uccaucauua ggaugaagaa aaaggacaaa     3420

uuauacuaua agguuaucgg ugugccaacu cgugucgcac acgaagugcu ccagagugau     3480

ggcaagauaa acauuuucac ccugaauaaa uucaucgagc agaaacuuac gaaggagaag     3540

guuaacaaaa agaccggggc gaucacacgg auuacugacu gguuugaacu ggugauagcc     3600

aaaguaaugu acggacaagu caucgaugac ggcggucagc ccuucauguu ggggagcucu     3660

acccucccuu acaaugugag agagcuguau cuagcuaagg aucuggugaa ggucauuaac     3720

aaaggcucca caucaaagag uagcuaccuu gacguuuaug augaacucgu gaaaucgcug     3780

aauaaguacu uuccaaugua ugacaggaac auguuccgcc agaaaaucac ugaugcccga     3840

gacaaguuug agaaguugcc caaaagagau aagguaucca auggaaaacu gcagcaaauu     3900

ggccagguga ccaucauaga ccggaucuua auugggcucc auacgaacgc aggagucucu     3960

aaucugaagg aucuuggcau cgcuacaccg uucgguuuua ugcagcaggg gaacggaauu     4020

agccugaccu caacugccaa aaucauauac caaaguucca caggcuuguu cgaaaagucu     4080

aaggcacucg acgaccugaa cccucuagcc uga                                  4113


<210>  3124
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3124
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acuaugcaag aggaaaagua cuaucaugag aaauucccga ccaucuacca ccuucgcaag      420

gaacuggcag acaagaaaga gaaggccgau uugcgacuca uuuaccuggc ucuggcacau      480

aucauuaaau uuagagguca cuuccucauc gaggacgaua gguuugacgu gcggaacaca      540

gacauacaga agcaguauca ggccuucuua gaaauuuuug auaccacuuu cgagaauaac      600

gaccugcuuu cucaaaaugu cgauguggaa gcuaucuuga cagacaaaau uucgaaguca      660

gccaaaaagg aucguauccu ggcgcgcuac cccaaccaaa agagcaccgg gaucuuugca      720

gaguuccuaa aacucauugu uggaaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacgc cucuucaguu cgcuaaagac aguuaugaug aggaccugga gaauuugcuc      840

ggccagauag gcgaugaauu ugccgaccug uucuccgcag cuaagaaguu auacgacucu      900

gugcugcuca gcgggauccu uacugucacc gaucugucca caaaagcccc auugucagcg      960

aguaugauuc agagauauga cgagcacagg gaagaucuaa agcagcugaa acaguuugug     1020

aaggccagcc uccccgagaa auaccaagaa aucuucgcag acucuuccaa ggauggauau     1080

gcugguuaca uagagggcaa gacuaaccag gaggccuuuu acaaauaucu gucaaagcug     1140

cugaccaaac aggaagggag cgaguacuug cucgaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaac gaacauuuga uaacggaagu aucccucacc agguacauuu aaccgaacug     1260

cgcgcuauca uuagaaggca gucugaguau uacccauucc uaaaggagaa ccucgaccgg     1320

aucgaaaaga uucugacguu ucgcauaccc uauuacguug gcccucuugc cagagagaag     1380

uccgauuucg cauggaugac uaggaaaaca gacgauagca uccguccaug gaauuuugaa     1440

gacuuggugg auaaggagaa aucggccgag gcuuucauuc accggaugac caacaaugac     1500

cuguaucucc cggaagagaa gguccugccc aaacauucuc uuaucuacga aaaguucacu     1560

guguacaacg agcugacaaa ggugcgauuu uuggcggaag guuucaaaga cuuucaguuc     1620

cucaauagaa agcaaaaaga gaccaucuuu aacucccugu ucaaggagaa aaggaagguc     1680

acggaaaagg auauuauauc auuuuuaaau aaaguugacg gguaugaggg aaucgcaauu     1740

aagggcaucg aaaaacaguu caacgccagu cugagcaccu accacgaucu aaagaaaauu     1800

cucggcaagg acuuucugga uaauacagac aacgagcuua ucuuggaaga uauagugcag     1860

acucugaccc ucuucgagga ccgcgagaug auccggaaga gacuggauau uuauaaagac     1920

uuuuucacag aaucccaguu aaagaaacug uacaggcgcc acuauacugg guggggacga     1980

cuuucugcua aguugaucaa ugguauucgg aacaaagaga aucaaaagac cauccucgau     2040

uaccugauag acgauggcag cgccaaccgu aauuuuaugc agcuaauuaa ggacgcaggg     2100

cugucauuca aaccuaucau ugacaaggcc agaacaggaa gucauuccga uaaccucaaa     2160

gaaguagugg gcgagcuggc ugguagccca gccaucaaga aagggauacu ucagucuuug     2220

aagaucgucg acgaacuggu gaaaguuaug ggcuaugagc ccgagcagau uguggucgaa     2280

auggcaaggg agaaccaaac cacugcgaag ggauuaucac gccuccggca gagacugacg     2340

acacuuaggg aaagucuggc uaaucuuaag agcaacaucc ucgaggagaa aaagccuaaa     2400

uacgugaagg aucagguaga aaaucaccau cuguccgacg aucgacuauu ccuguacuau     2460

cuccagaacg gcaaagacau guacaccgau gacgagcugg auauugacaa ucuuucucaa     2520

uaugauaucg acuugauaau uccacaggcc uuuaucaagg acgauucgau cgacaaccgc     2580

guucugguga gcuccgcaaa gaaucggggg aaaucagaug acguccccag uguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa uuacucgacg ccaagcugau gucucagaga     2700

aaguacgaua accuuacuaa agcugagagg ggaggucuga ccagcgacga uaaggcccgu     2760

uuuauccaac gccagcuagu ugaaacacgg cagauuacca aacacguggc aagaauacuc     2820

gacgagaggu ucaauaacga gcuggauucc aagggccgac ggauccgcaa aguaaagauu     2880

gucacuuuga agucaaaucu ggugagcaac uuuagaaaag aguucggguu uuauaagauc     2940

agggaaguua auaacuacca ucacgcucac gacgccuauu uaaaugcggu ggucgccaaa     3000

gcuauucuca caaaguaccc gcagcuggaa ccugaguucg uguacggaga uuaucccaaa     3060

uacaacucuu auaagacgcg gaaguccgca accgaaaaau uguuuuucua caguaacauc     3120

augaauuucu ucaagacuaa aguaacacug gccgacggca ccgugguugu caaggacgau     3180

auagagguga acaaugacac aggugagauc gugugggaua aaaagaaaca uuucgcuacu     3240

guccgaaagg uuuugagcua uccacaagug aacauuguga agaaaaccga aauccagacc     3300

gggggcuuuu cuaaggaguc cauucucgcc cacggaaauu cagacaaacu gaucccuaga     3360

aagacgaaag auauauaccu agaccccaag aaguauggcg gguucgauag uccaauugua     3420

gcauacagcg uccugguggu ugcggacauc aaaaagggaa aagcccagaa acuuaaaaca     3480

gugacugaac uccuggguau caccauuaug gagcgcucga gguuugaaaa gaaccccucc     3540

gcuuucuugg agucuaaggg cuaccugaau auacgugaug acaaauuaau gauccucccu     3600

aaguauagcc uguuugagcu ugaaaacggg cggagaaggc uguuggccuc agcagaugag     3660

cuccagaaag gaaaugaacu ggcucuacca acacaauuca ugaaguuucu guaccuugcc     3720

agucgcuaua acgagcucaa aggcaagccg gaggaaauug agcagaagca ggaauuuguc     3780

gugcagcaug uuuccuacuu ugacgauauc cugcaaauua ucaaugacuu cucuaaccga     3840

gugauauugg cagacgccaa uuuagagaaa aucaacaagc uguaucagga uaauaaagaa     3900

aacauucccg uagacgagcu cgcuaacaau aucauuaacc uguuuacuuu caccagccuu     3960

ggugcgccug ccgcauuuaa guucuucgau aaaaucgucg acagaaagcg guacacgucc     4020

acaaaagagg ugcuuaauuc aaccuugaua caccagagca uuacugggcu auaugaaaca     4080

aggaucgauc ugggcaagcu cggagaggac uga                                  4113


<210>  3125
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3125
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucauaacug augacuauaa gguucccgcu aaaaagauga aagugcucgg uaacacggau      120

aagaaguaca ucaaaaagaa ucuucugggc gcauuguuau ucgacucugg ggagaccgcc      180

gaagcgacaa gacugaaaag gacugcucgg cgccgauaua cccguagaaa gaacaggcua      240

cgguaccucc aggagauuuu ugccgaagag augacaaaag uagaugaauc auucuuuuau      300

cgccuggacg agaguuuccu uaccacugau gagaaggacu uugaaagaca cccuaucuuc      360

ggaaauaagg cagaggaaga ugccuaccau caaaaauuuc caacaauuua ucaccugagg      420

aacuacuugg cugacagcuc cgagaaggca gaucuccggc ugguguaccu ggcccucgcu      480

cauaugauca aauaucgcgg ccacuucuua auagagggua agcugaaugc cgaaaacacc      540

gacguccaga aacuuuuuac ggauuucgug gggguuuacg accgaacuuu ugacgauucu      600

cauuugucgg agaucaccgu ggacgucgcg ucaacacuga cugaaaagau uagcaaaagu      660

agaaggcuag agaaucucau caaguauuac ccgaccgaaa agaaaaacac acuguucgga      720

aaucuuauug cacuggccuu gggccuccag cccaacuuua agaugaauuu caaacugucc      780

gaggaugcua aguuacaguu cucuaaagac accuaugagg aagaucugga ggaacuccuu      840

ggcaagaucg gggacgauua cgccgaccug uuuacgagcg caaagaacuu guaugaugcu      900

auacuacugu ccgguauucu cacuguggac gauaauucaa caaaagcccc ucugagugcg      960

agcaugauca agcgguacgu agagcaccac gaagaccuug agaaacugaa ggaguucauu     1020

aaagccaaca agucugaauu guaccaugac aucuuuaagg auaaaaacaa gaauggauau     1080

gcaggcuaca ucgagaacgg gguuaaacaa gacgaguucu auaaguaccu caaaaauacc     1140

cuguccaaga uugauggauc agacuauuuu cuugauaaga uagaacguga agacuuccug     1200

cgcaaacaga gaacuuuuga uaacggcagc aucccacacc agauucaucu acaggagaug     1260

cacgcuaucc ucaggcggca aggugacuac uaucccuucc ugaaggagaa acaggaucga     1320

auagaaaaga uucuuacauu ucgcaucccu uacuacgugg ggccauuggu cagaaaagac     1380

aguagguucg ccugggcuga guaucggucu gaugaaaaga ucacccccug gaauuuugac     1440

aaagugauug auaaggagaa guccgccgag aaauucauca cgcgcaugac ccugaacgac     1500

cucuaccugc cugaagagaa ggugcuucca aaacacagcc augucuauga aacauacgca     1560

guuuauaaug agcugacuaa gauuaaauac gugaacgaac agggaaagga gucguuuuuc     1620

gacucuaaua ugaagcagga gauauucgau cacguauuua aagaaaacag aaaggugacc     1680

aaagagaagu ugcucaauua ucugaacaaa gaguucccgg aauacaggau caaggacuua     1740

auuggccugg auaaggaaaa uaaauccuuu aacgccucac uaggcacaua ccaugaccuc     1800

aagaaaaucc uggauaaggc uuuccuugac gauaaaguca augaggaggu gaucgaagac     1860

auuauaaaga cuuugacccu guuugaggau aaggacauga uccacgaacg ucuccaaaaa     1920

uauagugaua uuuucacagc gaaccagcug aagaaauuag agcggcgaca uuacaccggg     1980

uggggaagac ugagcuauaa guuaaucaac gguauuagga auaaagaaaa caauaagacu     2040

aucuuggacu accucauaga ugacggcucc gcaaaccgca auuuuaugca gcugaucaac     2100

gacgauacgc uacccuucaa acaaauuauc cagaagucuc agguuguggg ggacgucgau     2160

gacauugagg ccgugguaca cgaucugccu ggaagcccag cuaucaagaa aggcauacuc     2220

cagucaguua agauugugga cgagcugguc aaagugaugg gugauaaucc cgacaacauc     2280

gucauugaaa uggcccggga gaaucaaaca accaacagag ggaggaguca gucccagcag     2340

cgccuuaaga aauugcaaag cucucugaag gaauuaggcu caaauauccu caacgaggag     2400

aagccuaguu auauagaaga uaaaguggag aauagccauc ugcagaacga ccagcuuuuu     2460

cuguacuaua uccagaaugg aaaggauaug uacacuggcg acgaauugga cauugaucac     2520

cucuccgacu acgauaucga ccacauuauc ccacaagcau ucauaaaaga ugacucuauu     2580

gauaaccgag uucugaccuc gagcgccaag aaccggggga aauccgacga ugugcccuca     2640

cuagacaucg uacgugcuag aaaggccgag ugggucaggc uguauaagag uggacucauc     2700

ucuaaacgca aguuugauaa ucugacaaaa gcagaacggg guggccuuac cgaggcggac     2760

aaggcugggu ucauuaaaag acaguuggug gagacuaggc agaucacaaa gcauguugcc     2820

cagauucugg augcacgauu uaacacggaa cacgacgaga augacaaggu gauacgcgau     2880

gucaaaguga ucaccuuaaa gagcaaccuc guaucccaau uccggaaaga cuucgaauuu     2940

uacaagguga gagagauuaa ugauuaucac caugcccacg acgcuuaccu gaacgccguu     3000

gucggaacug cacuucugaa aaaguauccg aagcuagcuu cagaguucgu guacggcgaa     3060

uauaaaaagu acgauaucag gaaauuuauu acaaauagcu cugacaaggc caccgcgaaa     3120

uacuucuuuu auuccaaccu caugaauuuc uuuaagacaa aagugaagua cgccgauggu     3180

acugucuucg agcguccuau cauagaaacc aacgcugacg gggagaucgc auggaauaag     3240

cagauugauu uugaaaaagu ucgcaaggug cugaguuauc cccaggugaa caucguaaag     3300

aaggucgaga cgcaaaccgg cggauucagc aaagaaucua uuuugccaaa gggcgacucc     3360

gauaagcuga ucccucggaa aacaaagaaa guguacuggg acacuaagaa auauggggga     3420

uuugauucac ccaccguugc cuacagugug uucgucgugg cugacguuga gaaggguaag     3480

gccaaaaagu uaaaaacagu gaaggagcuc guaggcauaa gcauuaugga aagaucguuc     3540

uuugaggaaa auccagucga guuccuggag aacaaagggu aucauaauau cagggaagac     3600

aaacuuauca agcugcccaa auacuccuug uuugaguucg aaggaggccg acggcgccuc     3660

cuggcaucug cgagcgagcu acagaagggu aacgaaaugg ugcugccugg guaccuugug     3720

gagcuccugu aucacgccca uagagcugau aacuuuaauu caacugagua cuugaacuau     3780

gucagugaac acaaaaagga guucgaaaaa guucuguccu gcguggagga cuuugccaau     3840

uuauacguag auguggaaaa gaaccucucu aaaauuaggg cagucgcuga cagcauggau     3900

aauuucucca uagaggagau cucaaacagc uuuauuaauc ugcuuacccu gacggccuug     3960

ggcgcaccag ccgacuucaa cuuucucgga gaaaagaucc cgcggaagcg auauacaagu     4020

accaaagagu gucugacugc uacacuaauu caccagucua ucaccggccu guacgaaacu     4080

agaauagauc uuuccaagcu cggggaggaa uga                                  4113


<210>  3126
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3126
augaagaacg ccaauaaagu gaccggcaag uacuaucugg gguucgacau cggaacaaac       60

agcgucgguu gggcuguuac ugaugagaau uacaaccucu gcaaauuuaa gaaaaagucc      120

auguggggca uuagacuuuu cgaagacgca aagacggccg aggaaaggcg ggggcagcgc      180

gcgucucgac guagacugca aaggcggaaa cagcgcauag auuuguuaca ggagaucuuu      240

gcugaagaga uuaauaaggu ggacgagacc uucuuuauca gacugaacga aucaaggcua      300

caccucgaug acaaaacagu aagugagaag cauccccugu ucauuggaag cgaauauucc      360

gagaaagagu acuaucggga auacccuacu aucuuucacc uucgcaagga gcugauggaa      420

ucuucggagc cacaugauau acgauuggug uaucucgcca uccaccauau ucugaagaau      480

agaggccacu uccugaucga cggugaacuc ucaaaagcaa acagcuuuga ugccgcuuuc      540

uaccagauga aggaggucuu auguaaugag cuggaccuug aaauugaugg ggagaacacc      600

acagaagugg agaggaccuu gcgggaccgu aauaucagua aauccgauaa gguuaaacug      660

cuagcagccc uccuggcuau gaaccaaaau gaaacugagu cuaaggagac aaagaagaag      720

auagaaaaca uuugcaaacu ucuggccgga aauaagggcg acaucagcaa aauuuuuggc      780

cagaccccgg agggggugga aaagacgucc uucucauuug gagaggcgga cuacgaugaa      840

aguauccgcc ccaacuugga ggagagcauc ccugaccagu guuucgucau ugauucuaua      900

aaagcacucu augacuggaa cgugcuggcc gauaucuuaa auggucagac uuaccugucc      960

ugcgcuaagg uaaaccaaua ugaaacccac aagaauaacc ucaaagagcu uaagcuguug     1020

guuaauagau acuguucaag ggaagaguau aaacgguucu uuaagagcgc cguggagaaa     1080

gcaaacuaca guaauuauau uggcucugcu aagcagggga acaagaucau agacgucaaa     1140

cgaugcgccg aagaggauuu cuacaaggaa cuaaaaauuc ugcucuccaa gaucgagcca     1200

gaagaggcgg accgcgagcu guacgaaaga aucaaaaggg aggccgaaga gggaagccuu     1260

cugcccuugc agcggaauaa ggauaacucg guggugccua aucagaugca uaaggcagaa     1320

cucgcucuga uuuuagagaa cgccggcucu uaucuggguu uucuaaauga gaaagacgaa     1380

gaugggcucu ccccagcuga gaagaucauu gaacuguucu cauuucgcau acccuacuau     1440

gucggaccuc uuagugacag acacaaagac aggggcagca acgccuggau cguucguaag     1500

gcagagggcc ggauuuugcc auggaauuuc gaugaaaaag uggaccgaga gaaguccaac     1560

gaggccuuua ucgaaagaau gacaaacaaa uguacuuacc ugcucgggaa ggauguagug     1620

gcuaaaaauu cucugaucua uagccauuuc augguccuua acgagcugaa uaacuuguca     1680

gugugcggaa auaaaauuag uguucaagug aagcaggaca uauacaggga acucuuucag     1740

cgccacacca aagucuccaa gaaaaugcug uuacaauauu gucgggagaa gaugaugaac     1800

aaggaucugg aggaagagga ccuaagcggu uucgauggcg acuuuaaagc gaagcucucu     1860

ucauaccugg auuucgagaa aaagguguuu ggggcaaaaa uggaagaaga cagaaucagg     1920

aagauuacag aggauaucau ucgcuggaag accaucuacg gagacgaugg caaaaugcuu     1980

caggccguag uugagaagga auauccgggu gaguuggaca gugaccagcu gaaagcugug     2040

aagcaguucc gauacagcgg guggggcaau uucuccgaag aguuucucaa cggaauaguc     2100

ggcacggaaa aacccacugg gcaaauguuc acaaucauug aggcccugug gcagaccaau     2160

ugcaacuuaa ugcagcugcu uucuaagaau uuuacuuuca uggaggaaau cgagaagcac     2220

aaccgggaau uggcaggaca gauuacagau aucucguaug acaaacucgu gaaggaucug     2280

uacauaagcc cugccaauaa acgugcuguc uggcaaaccg ugagaauugc cgaggaaguu     2340

aagaaaauca uggguuguga gccagcaaag auuuuugugg agauggcgag gggcggggaa     2400

aaagagaaga agcgcacgau cucccggaaa aagaaacuac uggaacucua ugcugccugc     2460

aagaaagacg caagagauug gaacagggag auagaggacc gagaugaacg cgaguucaau     2520

ucagacaagc uguaccuuua uuacacccag augggacggu gcauguacag uggcgaaaga     2580

aucgauuugc augagcugau gucugccaac agcaaguggg acauugauca caucuauccc     2640

caguccaaag uaaaggacga uucauuagac aacagggucc ucagccugaa aaagauuaau     2700

aacgaaaaag guaauaagcc ucuuucucgu gacauccagc aaucccagca gaaguggugg     2760

acagagcugu ugcagcucga guuuauaacu aaaaagaaau acgaucgccu gaugcauaga     2820

ggggaacuaa gugaggagga acugagcggc uucauuaacc ggcaacucgu ggaaaccagg     2880

cagucuacaa aggcugccgc agagcugcuu ggacgaaugu auaauaacuc caaaguugug     2940

uacgucaagg cuccauuggu gucacaguuu cggaaggacg aucugaauau cuuaaaaagu     3000

cgccucgcca acgacuauca ccaugcgaag gaugccuacc ugaauaucgc uguaggcaac     3060

guguauaaug agaaauucac ugacaacccc cuuagaugga uugaaaagca cgcaggggag     3120

aauuacagca ucaacagggu uuuugauuac gacauugucg ccggagaugg ugcuccggug     3180

ugggccgcac cucugcggga cgagaaaaau aagcuacgaa agaacgaagu gggcgaaaaa     3240

uaugggggaa ccauagaugu caucagaaag acaguuaaac gcaacgacgu gcucuacacc     3300

gagcacacuu auuguggcaa gggugagcug uucaaugcga cgauugccaa aaaggacggg     3360

ggcgcugaaa uccccuugaa agccggacug caaacaggca aguacggggg auauaagucg     3420

ccaaacaccu ccuacuuugc agcuauugag uucgauggca aaaaggguga aagggugaaa     3480

aauaucaugg agguaccugu cuauauagcc aacaucuuac uccaugacau ugaugcauac     3540

auccguuacu gcgagacugu gaaggggcug aaaaauguua agauucuucg gcccuguauc     3600

aagaaaaacg cccugauauc uguggacgga uauccaauga gaauuagggg cgcuaaugaa     3660

aagaacaucu ugcucaaaaa uaaccugcag cccaucuucg gucgccacga ggaaaccguc     3720

cgaagaauug agaaguacgu ggagaaaaau caggaauuug agguugauga aaaguucgac     3780

ggccuaaacg aggcgaaucu gacagaucuu uaugacgaua uaaccgaaaa gcucugcggg     3840

ccuuacgugg agcggccaag caaccaggga gaauaucuga uggagaauag ggagaaauuu     3900

uugaacaucu cacugaagga aaaagccaag uuacucgcac aaaugauuaa acugguacgc     3960

ugugacacug agacaaaggc ugaucuucgu aguaucggcg ccuccaagaa ugccgggguc     4020

auuucuguga acaaaaacac gcugggaggc aagagauuga uccucauaaa ucagagcgug     4080

accggucugu ucgaaaaccg gaucgagcua uga                                  4113


<210>  3127
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3127
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acuaugcaag aggaaaagua cuaucaugag aaauucccga ccaucuacca ccuucgcaag      420

gaacuggcag acaagaaaga gaaggccgau uugcgacuca uuuaccuggc ucuggcacau      480

aucauuaaau uuagagguca cuuccucauc gaggacgaua gguuugacgu gcggaacaca      540

gacauacaga agcaguauca ggccuucuua gaaauuuuug auaccacuuu cgagaauaac      600

gaccugcuuu cucaaaaugu cgauguggaa gcuaucuuga cagacaaaau uucgaaguca      660

gccaaaaagg aucguauccu ggcgcgcuac cccaaccaaa agagcaccgg gaucuuugca      720

gaguuccuaa aacucauugu uggaaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacgc cucuucaguu cgcuaaagac aguuaugaug aggaccugga gaauuugcuc      840

ggccagauag gcgaugaauu ugccgaccug uucuccgcag cuaagaaguu auacgacucu      900

gugcugcuca gcgggauccu uacugucacc gaucugucca caaaagcccc auugucagcg      960

aguaugauuc agagauauga cgagcacagg gaagaucuaa agcagcugaa acaguuugug     1020

aaggccagcc uccccgagaa auaccaagaa aucuucgcag acucuuccaa ggauggauau     1080

gcugguuaca uagagggcaa gacuaaccag gaggccuuuu acaaauaucu gucaaaacuu     1140

cugaccaaac aggaagggag cgaguacuug cucgaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaac gaacauuuga uaacggaagu aucccucacc agguacauuu aaccgaacug     1260

cgcgcuauca uuagaaggca gucugaguau uacccauucc uaaaggagaa ccucgaccgg     1320

aucgaaaaga uucugacguu ucgcauaccc uauuacguug gcccucuugc cagagagaag     1380

uccgauuucg cauggaugac uaggaaaaca gacgauagca uccguccaug gaauuuugaa     1440

gacuuggugg auaaggagaa aucggccgag gcuuucauuc accggaugac caacaaugac     1500

cuguaucucc cggaagagaa gguccugccc aaacauucuc uuaucuacga aaaguucacu     1560

guguacaacg agcugacaaa ggugcgauuu uuggcggaag guuucaaaga cuuucaguuc     1620

cucaauagaa agcaaaaaga gaccaucuuu aacucccugu ucaaggagaa aaggaagguc     1680

acggaaaagg auauuauauc auuuuuaaau aaaguugacg gguaugaggg aaucgcaauu     1740

aagggcaucg aaaaacaguu caacgccagu cugagcaccu accacgaucu aaagaaaauu     1800

cucggcaagg acuuucugga uaauacagac aacgagcuua ucuuggaaga uauagugcag     1860

acucugaccc ucuucgagga ccgcgagaug auccggaaga gacuggauau uuauaaagac     1920

uuuuucacag aaucccaguu aaagaaacug uacaggcgcc acuauacugg guggggacga     1980

cuuucugcua aguugaucaa ugguauucgg aacaaagaga aucaaaagac cauccucgau     2040

uaccugauag acgauggcag cgccaaccgu aauuuuaugc agcuaauuaa ggacgcaggg     2100

cugucauuca aaccuaucau ugacaaggcc agaacaggaa gucauuccga uaaccucaaa     2160

gaaguagugg gcgagcuggc ugguagccca gccaucaaga aagggauacu ucagucuuug     2220

aagaucgucg acgaacuggu gaaaguuaug ggcuaugagc ccgagcagau uguggucgaa     2280

auggcaaggg agaaccaaac cacugcgaag ggauuaucac gcagucggca gagacucacg     2340

acacugaggg aaagccuugc uaaucugaag uccaacaucu uggaggagaa aaagccuaaa     2400

uacgugaagg aucagguaga aaaucaccau cucucugacg aucgacuguu ccuauacuau     2460

cugcagaacg gcaaagacau guacaccgau gacgagcucg auauugacaa ucugucgcaa     2520

uaugauaucg accacauaau uccacaggcc uuuaucaagg acgauagcau cgacaaccgc     2580

guucuugugu ccucagcaaa gaaucggggg aaaagugaug acguccccuc uguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa uugcuggacg ccaaguuaau gagccagaga     2700

aaguacgaua accucacuaa agcugagagg ggaggucuga ccuccgacga uaaggcccgu     2760

uuuauccaac gccagcuugu ugaaacacgg cagauuacca aacauguggc aagaauacug     2820

gacgagaggu ucaauaacga gcuagauuca aagggccgac ggauccgcaa aguaaagauu     2880

gucacucuca agagcaaucu ggugucuaac uuuagaaaag aguucggguu uuauaagauc     2940

agggaaguua auaacuacca ccacgcucau gacgccuauu ugaaugcggu ggucgccaaa     3000

gcuauucuga caaaguaccc gcaguuagaa ccugaguucg uguacggaga uuaucccaaa     3060

uacaacuccu auaagacgcg gaagagugca accgaaaaac ucuuuuucua cagcaacauc     3120

augaauuucu ucaagacuaa aguaacacug gccgacggca ccgugguugu caaggacgau     3180

auagagguga acaaugacac aggugagauc gugugggaua aaaagaaaca cuucgcuacu     3240

guccgaaagg uucuuucuua uccacaagug aacauuguga agaaaaccga aauccagacc     3300

gggggcuuuu ccaaggaguc aauucuggcc cauggaaaua gugacaaauu gaucccuaga     3360

aagacgaaag auauauaccu cgaccccaag aaguauggcg gguucgauag cccaauugua     3420

gcauacucgg uccugguggu ugcggacauc aaaaagggaa aagcccagaa gcuaaaaaca     3480

gugacugaac ugcuugguau caccauuaug gagcgcucca gguuugaaaa gaaccccucu     3540

gcuuuccucg agagcaaggg cuaccugaau auacgugaug acaaauugau gauccugccu     3600

aaguauucau uauuugagcu cgaaaacggg cggagaaggc ugcuugccag ugcaggagag     3660

cugcagaaag gcaaugaauu ggcucuccca acacaauuca ugaaguuucu guaccuagcc     3720

ucccgcuaua acgagcugaa agguaagccg gaggaaauug agcagaagca ggaauuuguc     3780

gugcagcacg uuucuuacuu ugaugacauc cuucaaauua ucaaugauuu cagcaaccga     3840

gugauacucg cagacgccaa ucuggagaaa aucaacaagu uguaucagga caauaaagaa     3900

aacauucccg uagaugaguu agcuaacaau aucauuaacc uguuuacuuu caccucccuc     3960

ggggcgccug ccgcauuuaa guucuucgac aaaaucgucg auagaaagcg guacacguca     4020

acaaaagagg ugcugaauag cacccuuaua caccagagua uuacuggccu guaugaaaca     4080

aggaucgacu ugggaaagcu aggcgaggau uga                                  4113


<210>  3128
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3128
augaccaaga ucaaagacga uuacauugug ggccuggaca uagggacaga uagcugcgga       60

ugggucgcca ugaacuccaa uaacgacauc cucaaacuuc aggguaaaac ugcuauuggc      120

ucuagacugu ucgaaggggg aaagucagca gccgaaaggc gguuguuucg cacgacccac      180

cgacguauca aaagaaggcg guggcgcuua aagcugcuag aggaguucuu ugaucccuau      240

auggcggagg uugacccuua cuucuuugcu agacucaagg aaaguggccu gagcccacuu      300

gauaaaagga agacaguguc cucuauugua uucccgacuu cggccgagga caaaaaguuu      360

uaugaugacu accccaccau cuaucaucug cgguacaaau ugaugacaga ggaugaaaag      420

uucgaccucc gcgaggugua ucuggcaaua caccauauca uuaaguaccg agguaauuuu      480

cuguacaaca ccucagucaa agauuucaag gccagcaaaa ucgacgugaa gaguuccauu      540

gaaaaacuca augaguuaua ugagaaccug gggcuugacu ugaauguuga auuuaacauc      600

ucuaauacug cugagauaga aaaggugcug aaagauaagc aaauuuucaa gagagacaaa      660

gucaagaaaa ucgcagagcu auuugccauu aagacagaua acaaagaaca gagcaagagg      720

aucaaggaca ucuccaaaca gguggcuaau gccguacucg gauacaagac ccgguucgau      780

acgauugcgc ugaaagagau aucaaaggac gagcuuagug auuggaacuu caaacugagc      840

gacaucgaug cagacucuaa guuugaagcc uugaugggca aucucgauga gaacgaacag      900

gcuauucugu uaacuaucaa ggagcuguuc aaugaaguua cccucaacgg cauaguggag      960

gacgggaaca cacuuuccga gucaaugauu aauaaauaua acgaccaccg ugaugaccug     1020

aaguugcuaa aagaagucau cgagaaucau aucgaucgca agaaagccaa ggaacuggca     1080

cucgcuuacg accuguaugu gaacaauaga cacggaaggc uucugcaagc caagaaaaag     1140

uuggguaaaa uuaagccucg gagcaaagag gauuuuuaca agguggucaa caaaaaucuc     1200

gacgauaguc gagcgucuaa ggaaaucaag aaaaagauug agcuggacuc cuucaugcca     1260

aaacagcgca cuaacgccaa uggcguuaua cccuaucagu uacagcaacu ggagcuagau     1320

aagaucauug aaaaccagag caaauacuac ccuuuucuca aggagaucaa uccagugucg     1380

ucucaccuga aggaagcacc cuauaaacuu gacgaguuga ucagauucag gguaccuuac     1440

uauguggggc cacugauuuc cccgaacgag ucaaccaagg auauacagac uaaaaagaau     1500

cagaacuuug cuuggaugau ccggaaagaa gagggacgca uuacccccug gaauuucgac     1560

caaaaggucg auagaaucga aagugccaac aaguuuauua aaaggaugac gacuaaggac     1620

acauaccucu ucggcgagga cgugcugccu gcuaacagcc uucuguauca gaaauuuacc     1680

guuuugaaug aacucaacaa uauccguaua aacgguaagc ggaucuccgu ggaucugaaa     1740

caggagauuu acgagaauuu auucaagaag cauacuacag ucaccgugaa aaagcuggaa     1800

aacuaucuaa aagagaauca caaccucgua aagguugaaa ucaaagggcu ggccgacgag     1860

aagaaauuua auucuggacu uacgaccuac aaccgauuca agaauuugaa cauuuucgau     1920

aaucaaaucg acgaucugaa guacagaaac gacuuugaaa agauaauuga guggagcaca     1980

aucuucgagg auaagucaau uuauaaagaa aagcucagga guaucgacug gcugaaugag     2040

aaacagauaa acgcauuauc caacauccgc cugcagggcu ggggccggcu uagcaagaag     2100

uugcucgccc agcugcauga ucacaauggg caaacuauua ucgaacagcu augggacucu     2160

cagaacaauu uuaugcagau ugugacccaa gcugauuuca aagacgcgau cgcaaaggcc     2220

aaccagaauc ugcucgucgc uacaucagug gaggauauac ugaacaaugc cuacacaagu     2280

ccagcaaaca aaaaggccau uagacagguc aucaaagugg uugacgacau cgugaaggcu     2340

gccagcggaa agguacccaa acagauugca aucgaauuua ccagggaugc ggacgagaau     2400

ccuaagcgcu cccaaacacg agguucuaaa cuucagaagg ucuauaaaga uuugucgacc     2460

gagcuggcua gcaagacuau ugccgaagag uuaaacgaag caauaaagga caaaaagcuc     2520

gugcaggaua aauacuaucu guacuucaug cagcuuggcc gggacgccua uacgggggag     2580

ccaaucaaua uugaugagau ccaaaaguac gacauugauc acauccugcc ccaguccuuu     2640

auaaaagacg augcuuugga caaccguguu cucgugucaa gagccgucaa uaacggaaag     2700

agugacaaug ugccgguaaa gcuguucggc aacgaaaugg cagcuaaccu agguaugaca     2760

aucaggaaaa ugugggagga auggaagaau auugggcuga ucucuaaaac caaguacaac     2820

aaucuccuga cugauccuga ccauauuaac aaauauaaga gcgccggcuu uauccgccgg     2880

cagcuugugg agaccuccca aauaauuaaa uugguuucaa caauccugca gagcagauac     2940

cccaauaccg aaaucauuac ugucaaggcg aaguauaacc acuacuuaag ggagaaauuc     3000

gaucucuaua agucucgaga ggugaaugac uaccaucacg ccauagaugc uuaucugucc     3060

gcaaucugug gaaaccuucu guaccagaau uacccaaacc uacgccccuu uuucguguau     3120

ggccaguaca aaaaguuuag uagcgacccu gauaaagaaa aggagauuuu caauaagaca     3180

cggaaauucu cuuuuaucuc ccaaauucuc aagaacaaau cagaaaauag uaaggagauc     3240

gccgaaaaac ugaagagagc uuaucaguuc aaguacaugu uggucagcag ggagacggag     3300

accaaagacc aggaaauguu uaagaugacu guuuucccac gugagacacc cgggaaccug     3360

auaccuaaaa agaaaggaau ggccccagaa aucuauggug gcuacaccaa aaauucggau     3420

gcauauaugg ugauugugaa gaucgacaag aagaaagaga cagaauaccg cauuuuaggg     3480

aucccgacuc gggagcucgu aaaccugaag aaagcggaga aggaagauca uuacgagucc     3540

uaucuuaaag aaauacuggc ccccagaauu uuguacaaua agaacggaaa gagggacaaa     3600

aagaucaccu cuuuugagau cgucaaaagc aagauuccuu auaaacaggu gauccaagau     3660

ggcgacaaga aguucaugcu cgguucaagu acguacguuu auaacgcuaa acagcugacc     3720

cuauccacag agucuaugaa ggccauaacu aauaacuuug acaaagauag cgacgaaaau     3780

gaugcacuga uuaaggcuua cgacgagauc cuugauaaag uggacaagua ucucccacug     3840

uucgauauua acgccuuucg aaagaaauug cacuccgggc gggaaaaguu cgucgagcug     3900

ucauuagaag acaaaaagga uaucauacuc aaagugcuga uuggccuuca cgacaauccc     3960

gcaaccagcg aucugaagac auugggauuu aguucuacug agcucggcaa gaugcagaaa     4020

aacuccggga ucguucugag cggaaaugcc aagcuaaucu accagucgcc uaccggucug     4080

uucaaaaagu cagugaaaau uucugaccuu uga                                  4113


<210>  3129
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3129
augaccaaga ucaaagacga uuacauugug ggccuggaca uagggacaga uagcugcgga       60

ugggucgcca ugaacuccga caaugauauc cucaaacuuc aggguaaaac ugcuauuggc      120

ucucaccugu ucgaggcagg gaagucagcc gcggacagaa gguuguuucg gacgacccau      180

cgccgaauca aacguagaag guggcgguua aagcugcuag aagaguucuu ugaucccuau      240

auggcuaagg uugacccuua cuucuuugcc cgccucaaag aaaguggacu gagcccacuu      300

gauaagagaa agaacguguu cuccauugua uuuccgacag cagccgagga caagaaauuc      360

uaugaugacu accccacuau cuaucaccug agguacaagu ugaugaccga agaugagaag      420

uuugaccucc gggaggugua ucuggcuaua caucacauca uuaaauaccg cggcaauuuc      480

cuguacaaca caucugucaa ggacuuuaaa gcaucgaaga ucgaugugaa aucaagcauu      540

gaaaagcuca augaguuaua ugaaaaccug ggucuugacu ugaauguuga guucaacauc      600

aguaauaccg ccgagauaga aaaagugcug aaggauaagc aaauuuuuaa acgagacaag      660

gucaaaaaga ucgcugagcu auucgccauu aaaacugaua acaaggaaca guccaagaga      720

aucaaagaca ucucuaaaca gguggcgaau gcaguacucg gguacaaaac aagguucgau      780

accauugccc ugaaggaggu uagcaaagac gaacuuuccg auuggaacuu uaagcuguca      840

gacauagaug cugacaguaa guucgaggcc uugaugggaa aucucgacga gaacgaacag      900

gcaauccugu uaacgauuaa agagcuguuu aaugcuguga cucucaacgg caucgucgaa      960

gauggcaaca cccuuagcga gucuaugaua aauaaguaua acgaccaucg ggaugaccug     1020

aaauugcuaa aggaagugau ugagaaucac aucgaucgua aaaaggccgu gaagcuggcg     1080

cucgccuacg accuguaugu caacaaucgc cacgggcaac uuauccaggc aaagaagaaa     1140

cugggaaaga uuaaaccugg uuccaaggag gauuucuaca aaguugugaa caagaauuug     1200

gacgauucac aggcuagcaa ggaaaucaaa aagaaaauug agcucgacag uuuuaugcca     1260

aagcagagaa caaacgccaa uggcguaaua cccuaucaac ugcaucaguu agaacuggau     1320

aaaaucauug agaaccaguc uaaguacuac ccuuuccuaa aggaaaucaa uccagugucc     1380

agccaccuca aagaggcucc cuauaagcug gacgagcuua ucagguuucg ggucccuuac     1440

uauguggggc cauugauuuc gccgaacgaa ucuacuaaag auauacagac caagaaaaau     1500

caaaacuucg ccuggaugau ccgaaaggag gaaggacgca uuacacccug gaauuuugac     1560

cagaagguug acagaaucga guccgcaaac aaauucauua agaggaugac cacgaaagau     1620

acuuaccugu uuggcgagaa cgugcucccu gccaauucac ugcuuuauca gaaguucaca     1680

guccugaacg aauugaauaa cauccggaua aaugguaaac gcaucagugu ggaccucaag     1740

caagagauuu acgaaaaccu guuuaagaaa cauaccacug uaacaguuaa gaaauuagag     1800

aauuaucuga agcagaacca caaucuagug aaagucgaaa ucaaggggcu cgcugaugag     1860

aaaaaguuca acagcggacu gaccacguac aauagauuca agaaccuuaa uauuuuugac     1920

aaccagaucg augacuugaa auacaggaag gauuucgaga aaauaauuga augguccacc     1980

aucuuugagg acaagucuau uuauaaagaa aagcugcgua gcaucgauug gcucaaugag     2040

aagcagauaa acgcgcuguc aaacauccgg uuacaaggcu ggggccgacu gaguaaaaag     2100

uugcuugcac agcuccacga ccauaauggg cagacaauua ucgaacagcu gugggauucc     2160

caaaacaauu ucaugcagau ugugacucag gccgacuuua aagaugcuau cgccaaggca     2220

aaccagaauc uacuggucgc caccagcgug gaggacauac ucaacgacgc uuacacaucu     2280

ccagccaaua aaaaggcaau uagacaaguu aucaaggugg uagaugacau cgucaaagcg     2340

gcuucaggaa aggugcccaa acagauugcc aucgaguuca cuagggaugc agacgauaac     2400

ccuaagcgca gucagacccg ggguagcaaa cugcagaagg uuuauaagga ccuuuccaca     2460

gaauuggccu cuaaagcuau ugccgaggaa cugaaugagg caauaaagga uaaaaaguua     2520

gugcaagaca aauacuaucu cuacuuuaug cagcugggca gagaugcuua uaccggggaa     2580

ccaaucaaca uugacgagau ccagaaguac gauauugacc acauccuucc ccaaucguuc     2640

auaaaggacg augcccugga caauaggguc uuggugagca aagcgguaaa caaugauaag     2700

uccgacaacg ugccgguuaa acucuuugga aaugagaugg ccggcaaccu ggguaucacu     2760

auucgcaaga ugugggaaga guggaaaaac aucgggcuaa uuucaaagac gaaauacaau     2820

aaccugcuca cagauccuga ccauaucaau aaguaugaaa gugcuggcuu cauacgacgg     2880

cagcuggucg agaccucuca gauuaucaaa cuugugagca cuaucuugca gucccguuac     2940

cccaacaccg agauuauaac agugaaagca aaguauaauc acuaccugag agaaaaauuu     3000

gauuuauaua agucaaggga ggucaacgac uaccaucacg ccaucgaugc uuaucucagc     3060

gccauuugug gaaaucugcu uuaccaaaac uacccaaauc ugcgcccuuu cuuuguuuau     3120

ggccaguaca aaaaguucuc uuccgacccc gauaaggaaa aagagaucuu caacaagacc     3180

cggaaauuua guuucauuag ccagauccua aagaauaaau cugaaaacuc caaggagaua     3240

gcagagaagc ucaaaagagc guaucaguuu aaguacaugc uggugucaag ggaaacugag     3300

acaaaagacc aagaaauguu caagaugacg guguuuccac gagagacccc cgggaauuug     3360

aucccuaaaa agaagggaau ggccacugaa auuuauggug gcuacacaaa gaacagugau     3420

gcuuauaugg uaaucgucaa gauugacaaa aagaaagaga ccgaguaccg cauccugggg     3480

auaccaacac gggaauuagu gaaucucaag aaagccgaga aggaagacca cuacgagagc     3540

uaucugaagg aaauucuugc accgagaauc cuguacaaca aaaauggaaa gagggauaaa     3600

aagaucacuu cguucgagau uguuaaaucc aagauccccu auaaacaggu gauacaggac     3660

ggcgauaaaa aguuuauguu ggguucuagc accuacgucu auaacgcuaa acagcucacg     3720

cugucaaccg agaguaugaa ggccauuaca aacaauuucg acaaagauuc cgacgaaaac     3780

gaugcacuaa ucaaggccua cgacgagauu cuggauaagg uggacaaaua ucuuccucuc     3840

uuugauauca augcuuuccg uaagaaacug cauucugggc gcgaaaaguu uguugaguug     3900

agccuggagg acaaaaagga uauaauuuua aaggugcuca ucggccugca cgacaaccca     3960

gcgacuuccg accuuaaaac ccugggauuc ucaagcacag aauugggcaa gaugcaaaag     4020

aauaguggga ucguacucuc uggaaacgcc aagcugauuu accagucccc cacuggucua     4080

uucaaaaaga gcgucaaaau cucggaucug uga                                  4113


<210>  3130
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3130
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acuaugcaag aggaaaagua cuaucaugag aaauucccga ccaucuacca ccuucgcaag      420

gaacuggcag acaagaaaga gaaggccgau uugcgacuca uuuaccuggc ucuggcacau      480

aucauuaaau uuagagguca cuuccucauc gaggacgaua gguuugacgu gcggaacaca      540

gacauacaga agcaguauca ggccuucuua gaaauuuuug auaccacuuu cgagaauaac      600

gaccugcuuu cucaaaaugu cgauguggaa gcuaucuuga cagacaaaau uucgaaguca      660

gccaaaaagg aucguauccu ggcgcgcuac cccaaccaaa agagcaccgg gaucuuugca      720

gaguuccuaa aacucauugu uggaaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacgc cucuucaguu cgcuaaagac aguuaugaug aggaccugga gaauuugcuc      840

ggccagauag gcgaugaauu ugccgaccug uucuccgcag cuaagaaguu auacgacucu      900

gugcugcuca gcgggauccu uacugucacc gaucugucca caaaagcccc auugucagcg      960

aguaugauuc agagauauga cgagcacagg gaagaucuaa agcagcugaa acaguuugug     1020

aaggccagcc uccccgagaa auaccaagaa aucuucgcag acucuuccaa ggauggauau     1080

gcugguuaca uagagggcaa gacuaaccag gaggccuuuu acaaauaucu gucaaaacuu     1140

cugaccaaac aggaagggag cgaguacuug cucgaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaac gaacauuuga uaacggaagu aucccucacc agguacauuu aaccgaacug     1260

cgcgcuauca uuagaaggca gucugaguau uacccauucc uaaaggagaa ccucgaccgg     1320

aucgaaaaga uucugacguu ucgcauaccc uauuacguug gcccucuugc cagagagaag     1380

uccgauuucg cauggaugac uaggaaaaca gacgauagca uccguccaug gaauuuugaa     1440

gacuuggugg auaaggagaa aucggccgag gcuuucauuc accggaugac caacaaugac     1500

cuguaucucc cggaagagaa gguccugccc aaacauucuc uuaucuacga aaaguucacu     1560

guguacaacg agcugacaaa ggugcgauuu uuggcggaag guuucaaaga cuuucaguuc     1620

cucaauagaa agcaaaaaga gaccaucuuu aacucccugu ucaaggagaa aaggaagguc     1680

acggaaaagg auauuauauc auuuuuaaau aaaguugacg gguaugaggg aaucgcaauu     1740

aagggcaucg aaaaacaguu caacgccagu cugagcaccu accacgaucu aaagaaaauu     1800

cucggcaagg acuuucugga uaauacagac aacgagcuua ucuuggaaga uauagugcag     1860

acucugaccc ucuucgagga ccgcgagaug auccggaaga gacuggauau uuauaaagac     1920

uuuuucacag aaucccaguu aaagaaacug uacaggcgcc acuauacugg guggggacga     1980

cuuucugcua aguugaucaa ugguauucgg aacaaagaga aucaaaagac cauccucgau     2040

uaccugauag acgauggcag cgccaaccgu aauuuuaugc agcuaauuaa ggacgcaggg     2100

cugucauuca aaccuaucau ugacaaggcc agaacaggaa gucauuccga uaaccucaaa     2160

gaaguagugg gcgagcuggc ugguagccca gccaucaaga aagggauacu ucagucuuug     2220

aagaucgucg acgaacuggu gaaaguuaug ggcuaugagc ccgagcagau uguggucgaa     2280

auggcaaggg agaaccaaac cacugcgaag ggauuaucac gcagucggca gagacucacg     2340

acacugaggg aaagccuugc uaaucugaag uccaacaucu uggaggagaa aaagccuaaa     2400

uacgugaagg aucagguaga aaaucaccau cucucugacg aucgacuguu ccuauacuau     2460

cugcagaacg gcaaagacau guacaccgau gacgagcucg auauugacaa ucugucgcaa     2520

uaugauaucg accacauaau uccacaggcc uuuaucaagg acgauagcau cgacaaccgc     2580

guucuugugu ccucagcaaa gaaucggggg aaaagugaug acguccccuc uguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa uugcuggacg ccaaguuaau gagccagaga     2700

aaguacgaua accucacuaa agcugagagg ggaggucuga ccuccgacga uaaggcccgu     2760

uuuauccaac gccagcuugu ugaaacacgg cagauuacca aacauguggc aagaauacug     2820

gacgagaggu ucaauaacga gcuagauuca aagggccgac ggauccgcaa aguaaagauu     2880

gucacucuca agagcaaucu ggugucuaac uuuagaaaag aauuuggguu uuauaagauc     2940

agggagguua auaacuacca ccacgcucau gacgccuauu ugaaugcggu ggucgccaaa     3000

gcuauucuga caaaguaccc gcaguuagaa ccugaguucg uguacggaga uuaucccaaa     3060

uacaacuccu auaagacgcg gaagagugca accgaaaaac ucuuuuucua cagcaacauc     3120

augaauuucu ucaagacuaa aguaacacug gccgacggca ccgugguugu caaggacgau     3180

auagagguga acaaugacac aggugagauc gugugggaua aaaagaaaca cuucgcuacu     3240

guccgaaagg uucuuucuua uccacaagug aacauuguga agaaaaccga aauccagacc     3300

gggggcuuuu ccaaggaguc aauucuggcc cauggaaaua gugacaaauu gaucccuaga     3360

aagacgaaag auauauaccu cgaccccaag aaguauggcg gguucgauag cccaaaagua     3420

gcauacucgg uccugguggu ugcggacauu aagaaaggaa aggcccagaa acuaaagaca     3480

gugacugaac ugcuugguau caccaucaug gagcgcucca gguuugaaaa gaaccccucu     3540

gcuuuccucg agagcaaagg cuaccugaau auucgugaug acaaguugau gauacugccu     3600

aaauauucau uauuugagcu cgaaaacggg cggagaaggc ugcuugccag ugcaggagag     3660

cugcagaagg gcaaugaauu ggcucuccca acacaauuca ugaaauuucu guaccuagcc     3720

ucccgcuaua acgagcugaa ggguaagccg gaggaaaucg agcagaaaca ggaguucguc     3780

gugcagcacg uuucuuacuu ugaugacauu cuucaaauca uuaaugauuu cagcaaccga     3840

gugauccucg cagacgccaa ucuggaaaag auaaacaaau uguaucagga caauaaggaa     3900

aacauccccg uagaugaguu agcuaacaau auuaucaacc uguuuacuuu caccucccuc     3960

ggggcgccug ccgcauuuaa auucuucgac aagauugucg auagaaaacg guacacguca     4020

acaaaggagg ugcugaauag cacccuuauc cacaagagua uaacuggccu guaugaaaca     4080

aggauugacu ugggaaaacu aggcgaggau uga                                  4113


<210>  3131
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3131
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaggu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acucuucaag aggaaaagga cuaccaugag aaauucucua ccaucuauca ucugcgcaag      420

gaauuggcag auaagaaaga gaaggccgac cuccgacuga uuuacaucgc ucuggcacac      480

auuaucaaau uuagagguca cuuccucaua gaggaugacu cguuugacgu gaggaacaca      540

gauauuucaa agcaguacca ggacuucuua gaaaucuuua auaccacuuu cgagaacaau      600

gaucugcuua gccagaacgu cgacguggaa gccauuuuga cagauaaaau caguaagucc      660

gcuaaaaagg accggauccu ggcccaauau ccgaaucaga agucuaccgg gauuuuugcg      720

gaguuccuaa aacucauagu uggaaaccag gcagauuuca agaaauacuu uaaucuggaa      780

gacaagacgc cccuucaguu cgccaaagau agcuaugacg aggaucugga gaacuugcuc      840

ggccaaaucg gcgacgaauu ugcugaccug uucuccgccg caaagaaguu auacgauuca      900

gugcugcuca gugggauucu uacugucauc gaccugagca ccaaagcucc uuugucugcc      960

uccaugauac agcguuauga ugagcaucgc gaagaccuaa agcagcugaa acaguuugug     1020

aaggcgucac ucccagagaa auaccaagaa auuuucgccg auagcaguaa ggacggauau     1080

gcagguuaca ucgagggcaa gacaaaucag gaggcuuuuu acaaauaucu gucuaaacuu     1140

cugacuaagc aggaagauuc cgagaacuuc uuggaaaaga ucaaaaacga ggacuuucuc     1200

agaaagcaaa ggaccuucga uaaugggagc auuccccacc agguacaccu gacagaauua     1260

aaggccauca uucggcgaca gucggaguac uauccuuuuc ugaaagagaa ccaggaccgc     1320

auagaaaaga uccuaaccuu caggauucca uacuauaucg gaccccucgc uagagagaaa     1380

ucugauuuug ccuggaugac gcggaagacu gacgauucca uccgcccuug gaauuucgaa     1440

gaccugguug acaaagagaa gucagcagag gccuucauuc auagaaugac aaacaaugau     1500

uuuuaccuuc cagaagagaa aguguugccg aagcacaguc ugauauauga aaaguucacc     1560

gucuacaacg agcucacuaa agugagguac aagaaugaac aaggcgagac auacuuuuuc     1620

gacagcaaca ucaaacagga gauuuuugau gguguguuca aggaacaucg uaaagucucc     1680

aagaagaaac ugcuugacuu ucuggcuaag gaguaugaag aguuccggau cguugaugug     1740

auuggguugg acaaagaaaa uaaggcguuu aacgcaucuc ucggaaccua ucacgaucug     1800

gagaaaaucu uagacaagga uuuccuggac aaucccgaua acgagagcau acuagaagac     1860

aucguacaga cgcucacccu guuugaggau cgagaaauga uuaagaaaag acuugagaau     1920

uacaaggacu uguucacaga aucacagcug aaaaagcucu auaggcgcca cuacacuggc     1980

uggggccggc ugagugccaa auuaaucaac gggauuagag acaaggaguc ccaaaagacc     2040

auccuggauu aucuuauaga cgauggaagg agcaaucgca acuuuaugca guugauuaac     2100

gacgaugguc ucucuuucaa aucaaucauu aguaaggcuc aggccggcag ccauuccgac     2160

aaucugaaag agguggucgg ggaacuagca ggaucuccug ccaucaagaa aggcauacug     2220

cagucgcuca agaucgugga ugagcugguu aaagugaugg guuacgaacc agagcaaauu     2280

gucguggaga uggcucgaga aaaccagaca acuaaucagg ggcggcguaa cagcagacag     2340

agguacaaac uuuuggacga uggcguaaag aaucuggccu ccgacuuaaa cggaaauauc     2400

cucaaggagu aucccaccga uaaccaagca cugcagaaug aacgccuuuu ccuguacuau     2460

uugcagaacg gccgggacau guacacaggg gaggcgcucg acauugauaa ucugucacaa     2520

uaugacaucg aucacauaau uccucaggcu uuuaucaagg acgauaguau cgacaacaga     2580

guucuagugu cuagcgccaa aaauagggga aaguccgaug acgucccauc acuggaaauu     2640

gugaaagauu gcaaggucuu cuggaagaaa cuccuggacg caaaacuuau gagccagcga     2700

aaguacgaua acuugaccaa ggccgagcgc gguggccuga cuucugacga uaaagcucgg     2760

uuuauccaga gacaauuagu ggagacgagg cagauuacaa agcauguugc ccguauacuc     2820

gacgaacgcu ucaauaacga gcuggacucc aaggggcgga gaaucaggaa agugaagauu     2880

guaacccuua aaaguaaccu ggucagcaau uuucgaaagg aguucggauu uuauaaaauc     2940

cgggaaguga acaauuacca ccaugcacac gaugcuuacc uaaacgccgu uguggcgaag     3000

gccauucuca cuaaguaucc ccagcuggaa ccggaguucg ucuacggcga cuauccuaaa     3060

uacaauucuu auaagacccg caaauccgcu acagagaagu uguuuuucua cucaaacauc     3120

augaacuuuu ucaaaaccaa ggugacucug gcagauggua caguaguggu uaaggacgau     3180

auagaaguca auaaugacac gggggagauc gugugggaua aaaagaaaca cuucgccacc     3240

gugagaaagg ucuuaaguua uccccagaac aauauuguua aaaagacuga aauccaaaca     3300

ggcggauuua gcaaagaguc gauucucgcu cauggcaacu ccgacaagcu gaucccaagg     3360

aagaccaaag auauauaccu ugacccuaag aaauacgggg gauucgauuc ucccauugug     3420

gccuauagcg ugcugguagu cgcagacauc aagaaaggua aggcgcagaa guugaaaaca     3480

gugacugaac uccugggcau caccauuaug gagcggucac gauuugagaa gaauccaagu     3540

gccuuccuag aauccaaagg guaccugaac auaagagcug auaaacuuau cauucucccc     3600

aaguauucuc uguuugaguu ggaaaaugga cgcaggcguc uguuagccag cgcaggcgag     3660

cuccagaagg guaacgagcu ggcccuuccu acgcaguuca ugaaguuucu guacuuggcu     3720

ucccgguaua acgaaucaaa agggaagcca gaggaaaucg agaaaaagca agaguucguu     3780

aaucagcacg ugagcuacuu ugacgacauu cuccagcuga ucaacgauuu caguaaaaga     3840

gucauacuag cagacgccaa ucuggaaaag aucaacaaac uuuaucaaga uaauaaggag     3900

aacauuucug uggacgaacu cgcuaauaac aucauuaauc uguuuaccuu cacauccuug     3960

ggcgcgccgg ccgcauuuaa guucuucgau aaaaucguug acaggaagcg cuacacuagc     4020

accaaagagg uguuaaacuc gacacugaua caucagucaa uuacuggacu cuacgaaacc     4080

cgaaucgauc ugggcaaacu uggggaggac uga                                  4113


<210>  3132
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3132
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaggu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acucuucaag aggaaaagga cuaccaugag aaauucucua ccaucuauca ucugcgcaag      420

gaauuggcag auaagaaaga gaaggccgac cuccgacuga uuuacaucgc ucuggcacac      480

auuaucaaau uuagagguca cuuccucaua gaggaugacu cguuugacgu gaggaacaca      540

gauauuucaa agcaguacca ggacuucuua gaaaucuuua auaccacuuu cgagaacaau      600

gaucugcuua gccagaacgu cgacguggaa gccauuuuga cagauaaaau caguaagucc      660

gcuaaaaagg accggauccu ggcccaauau ccgaaucaga agucuaccgg gauuuuugcg      720

gaguuccuaa aacucauagu uggaaaccag gcagauuuca agaaauacuu uaaucuggaa      780

gacaagacgc cccuucaguu cgccaaagau agcuaugacg aggaucugga gaacuugcuc      840

ggccaaaucg gcgacgaauu ugcugaccug uucuccgccg caaagaaguu auacgauuca      900

gugcugcuca gugggauucu uacugucauc gaccugagca ccaaagcucc uuugucugcc      960

uccaugauac agcguuauga ugagcaucgc gaagaccuaa agcagcugaa acaguuugug     1020

aaggcgucac ucccagagaa auaccaagaa auuuucgccg auagcaguaa ggacggauau     1080

gcagguuaca ucgagggcaa gacaaaucag gaggcuuuuu acaaauaucu gucuaaacuu     1140

cugacuaagc aggaagauuc cgagaacuuc uuggaaaaga ucaaaaacga ggacuuucuc     1200

agaaagcaaa ggaccuucga uaaugggagc auuccccacc agguacaccu gacagaauua     1260

aaggccauca uucggcgaca gucggaguac uauccuuuuc ugaaagagaa ccaggaccgc     1320

auagaaaaga uccuaaccuu caggauucca uacuauaucg gaccccucgc uagagagaaa     1380

ucugauuuug ccuggaugac gcggaagacu gacgauucca uccgcccuug gaauuucgaa     1440

gaccugguug acaaagagaa gucagcagag gccuucauuc auagaaugac aaacaaugau     1500

uuuuaccuuc cagaagagaa aguguugccg aagcacaguc ugauauauga aaaguucacc     1560

gucuacaacg agcucacuaa agugagguac aagaaugaac aaggcgagac auacuuuuuc     1620

gacagcaaca ucaaacagga gauuuuugau gguguguuca aggaacaucg uaaagucucc     1680

aagaagaaac ugcuugacuu ucuggcuaag gaguaugaag aguuccggau cguugaugug     1740

auuggguugg acaaagaaaa uaaggcguuu aacgcaucuc ucggaaccua ucacgaucug     1800

gagaaaaucu uagacaagga uuuccuggac aaucccgaua acgagagcau acuagaagac     1860

aucguacaga cgcucacccu guuugaggau cgagaaauga uuaagaaaag acuugagaau     1920

uacaaggacu uguucacaga aucacagcug aaaaagcucu auaggcgcca cuacacuggc     1980

uggggccggc ugagugccaa auuaaucaac gggauuagag acaaggaguc ccaaaagacc     2040

auccuggauu aucuuauaga cgauggaagg agcaaucgca acuuuaugca guugauuaac     2100

gacgaugguc ucucuuucaa aucaaucauu aguaaggcuc aggccggcag ccauuccgac     2160

aaucugaaag agguggucgg ggaacuagca ggaucuccug ccaucaagaa aggcauacug     2220

cagucgcuca agaucgugga ugagcugguu aaagugaugg guuacgaacc agagcaaauu     2280

gucguggaga uggcucgaga aaaccagaca acuaaucagg ggcggcguaa cagcagacag     2340

agguacaaac uuuuggacga uggcguaaag aaucuggccu ccgacuuaaa cggaaauauc     2400

cucaaggagu aucccaccga uaaccaagca cugcagaaug aacgccuuuu ccuguacuau     2460

uugcagaacg gccgggacau guacacaggg gaggcgcucg acauugauaa ucugucacaa     2520

uaugacaucg aucacauaau uccucaggcu uuuaucaagg acgauaguau cgacaacaga     2580

guucuagugu cuagcgccaa aaauagggga aaguccgaug acgucccauc acuggaaauu     2640

gugaaagauu gcaaggucuu cuggaagaaa cuccuggacg caaaacuuau gagccagcga     2700

aaguacgaua acuugaccaa ggccgagcgc gguggccuga cuucugacga uaaagcucgg     2760

uuuauccaga gacaauuagu ggagacgagg cagauuacaa agcauguugc ccguauacuc     2820

gacgaacgcu ucaauaacga gcuggacucc aaggggcgga gaaucaggaa agugaagauu     2880

guaacccuua aaaguaaccu ggucagcaau uuucgaaagg aguucggauu uuauaaaauc     2940

cgggaaguga acaauuacca ccaugcacac gaugcuuacc uaaacgccgu uguggcgaag     3000

gccauucuca cuaaguaucc ccagcuggaa ccggaguucg ucuacggcga cuauccuaaa     3060

uacaauucuu auaagacccg caaauccgcu acagagaagu uguuuuucua cucaaacauc     3120

augaacuuuu ucaaaaccaa ggugacucug gcagauggua caguaguggu uaaggacgau     3180

auagaaguca auaaugacac gggggagauc gugugggaua aaaagaaaca cuucgccacc     3240

gugagaaagg ucuuaaguua uccccagaac aauauuguua aaaagacuga aauccaaaca     3300

ggcggauuua gcaaagaguc gauucucgcu cauggcaacu ccgacaagcu gaucccaagg     3360

aagaccaaag auauauaccu ugacccuaag aaauacgggg gauucgauuc ucccauugug     3420

gccuauagcg ugcugguagu cgcagacauc aagaaaggua aggcgcagaa guugaaaaca     3480

gugacugaac uccugggcau caccauuaug gagcggucac gauuugagaa gaauccaagu     3540

gccuuccuag aauccaaagg guaccugaac auaagagaug acaaacuuau gauccucccc     3600

aaguauucuc uguuugaguu ggaaaaugga cgcaggcguc uguuagcuag cgccggcgag     3660

cuccagaagg guaacgagcu ggcacuuccu acgcaguuca ugaaguuucu guacuuggcu     3720

ucccgguaua acgaaucaaa agggaagcca gaggaaauug agaaaaagca agaguucguu     3780

aaucagcacg ugagcuacuu ugacgauauc cuccagcuga uuaacgacuu caguaaaaga     3840

gucauccuag ccgaugcaaa ucuggaaaag auaaacaaac uuuaucaaga caauaaggag     3900

aacaucucug uggaugaacu cgccaauaac auuaucaauc uguuuaccuu cacauccuug     3960

ggcgcuccgg cggccuuuaa guucuucgac aaaauuguug auaggaagcg cuacacuagc     4020

accaaagagg uguuaaacuc gacacugauc caucagucaa uaacuggacu cuacgaaacc     4080

cgaauugacc ugggcaaacu uggggaggau uga                                  4113


<210>  3133
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3133
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acuaugcaag aggaaaagua cuaucaugag aaauucccga ccaucuacca ccuucgcaag      420

gaacuggcag acaagaaaga gaaggccgau uugcgacuca uuuaccuggc ucuggcacau      480

aucauuaaau uuagagguca cuuccucauc gaggacgaua gguuugacgu gcggaacaca      540

gacauacaga agcaguauca ggccuucuua gaaauuuuug auaccacuuu cgagaauaac      600

gaccugcuuu cucaaaaugu cgauguggaa gcuaucuuga cagacaaaau uucgaaguca      660

gccaaaaagg aucguauccu ggcgcgcuac cccaaccaga aaagcaccgg gaucuuugca      720

gaguuccuaa aacucauugu uggaaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacgc cucuucaguu cgcuaaagac aguuaugaug aggaccugga gaauuugcuc      840

ggccaaauag gcgaugaauu ugccgaccug uucuccgcag cuaagaaguu auacgacucu      900

gugcugcuca gcgggauccu uacugucacc gaucugucca caaaagcccc auugucagcg      960

aguaugauuc agagauauga cgagcacagg gaagaucuaa agcagcugaa acaguuugug     1020

aaggccagcc uccccgagaa auaccaagaa aucuucgcag acucuuccaa ggauggauau     1080

gcugguuaca uagagggcaa gacuaaccag gaggccuuuu acaaauaucu gucaaagcug     1140

cugaccaaac aggaagggag cgaguacuug cucgaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaac gaacauuuga uaacggaagu aucccucacc agguacauuu aaccgaacug     1260

cgcgcuauca uuagaaggca gucugaguau uacccacuac ucaaggagaa ccuggaccgg     1320

aucgaaaaga uucuuacguu ccgcauaccc uauuacguug gcccuuuggc cagagagaag     1380

uccgauuuug cauggaugac uaggaaaaca gacgauagca uccguccaug gaauuucgaa     1440

gaccuggugg auaaggagaa aucggccgag gcuuuuauuc accggaugac caacaaugac     1500

cucuaucugc cggaagagaa gguccuuccc aaacauucuc ugaucuacga aaaguucacu     1560

guguacaacg aguugacaaa ggugcgauuc cucgcggaag guuuuaaaga cuuccaguuu     1620

cugaauagaa agcaaaaaga gaccaucuuc aacuccuuau uuaaggagaa aaggaagguc     1680

acggaaaagg auauuauauc auuccugaau aaaguugacg gguaugaggg aaucgcaauu     1740

aagggcaucg aaaaacaguu uaacgccagu cuaagcaccu accacgaucu caagaaaauu     1800

cugggcaagg acuuccuuga uaauacagac aacgaguuga uccuggaaga uauagugcag     1860

acucucaccc uguuugagga ccgcgagaug auccggaaga gauuagauau uuauaaagac     1920

uucuuuacag aaucccagcu gaagaaacuu uacaggcgcc acuauacugg guggggacga     1980

uugucugcua agcucaucaa ugguauucgg aacaaagaga aucaaaagac cauccuggau     2040

uaccuaauag acgauggcag cgccaaccgu aauuucaugc agcugauuaa ggacgcaggg     2100

cucucauuua aaccuaucau ugacaaggcc agaacaggaa gucauuccga uaaccugaaa     2160

gaaguagugg gcgagcuugc ugguagccca gccaucaaga aagggauauu gcagucucug     2220

aagaucgucg acgaauuagu gaaaguuaug ggcuaugagc ccgagcagau uguggucgaa     2280

auggcaaggg agaaccaaac cacugcgaag ggacucucac gcagucggca gagacugacg     2340

acacuuaggg aaagccuggc uaauuugaag uccaacaucc ucgaggagaa aaagccuaaa     2400

uacgugaagg aucagguaga aaaucaccau cugucugacg aucgacuauu ccuguacuau     2460

cuccagaacg gcaaagacau guacaccgau gacgagcugg auauugacaa ucuuucgcaa     2520

uaugauaucg accacauaau uccacaggcc uucaucaagg acgauagcau cgacaaccgc     2580

guuuuggugu ccucagcaaa gaaucggggg aaaagugaug acguccccuc uguggaaauu     2640

gucaaggauu gcaaaguguu uuggaagaaa cuguuagacg ccaagcucau gagccagaga     2700

aaguacgaua accugacuaa agcugagagg ggaggucuua ccuccgacga uaaggcccgu     2760

uucauccaac gccagcuggu ugaaacacgg cagauuacca aacauguggc aagaauacua     2820

gacgagaggu uuaauaacga gcucgauuca aagggccgac ggauccgcaa aguaaagauu     2880

gucacucuua agagcaauuu ggugucuaac uucagaaaag aauuuggguu cuauaagauc     2940

agggagguua auaacuacca ccacgcucau gacgccuauc ugaaugcggu ggucgccaaa     3000

gcuauuuuaa caaaguaccc gcagcucgaa ccugaguuug uguacggaga uuaucccaaa     3060

uacaacuccu auaagacgcg gaagagugca accgaaaaac uguucuuuua cagcaacauc     3120

augaauuucu uuaagacuaa aguaacacuu gccgacggca ccgugguugu caaggacgau     3180

auagagguga acaaugacac aggugagauc gugugggaua aaaagaaaca cuucgcuacu     3240

guccgaaagg uucugucuua uccacaagug aacauuguga agaaaaccga aauccagacc     3300

gggggcuucu ccaaggaguc aauuuuggcc cauggaaaua gugacaaacu caucccuaga     3360

aagacgaaag auauauaccu ggaccccaag aaguauggcg gguuugauag cccaauugua     3420

gcauacucgg uccuaguggu ugcggacauc aaaaagggaa aagcccagaa gcugaaaaca     3480

gugacugaac uucucgguau caccauuaug gagcgcucca gguucgaaaa gaaccccucu     3540

gcuuuucugg agagcaaggg cuacuuggau auacgugacg auaaacugau gaucuuaccu     3600

aaguauucac ucuucgagcu ggaaaauggg cggagaaggc uucuggccag ugcaggagag     3660

uugcagaaag gcaacgaacu cgcucugcca acacaauuua ugaaguuccu auaccuggcc     3720

ucccgcuaua augagcuuaa agguaagccg gaggaaauug agcagaagca ggaauuuguc     3780

gugcagcacg uuucuuacuu cgacgauauc cuccaaauua ucaacgacuu uagcaaucga     3840

gugauacugg cagacgccaa cuuggagaaa aucaauaagu uauaucagga uaacaaagaa     3900

aauauucccg uagacgagcu ggcuaacaac aucauuaauc ucuucacuuu uaccucccug     3960

ggggcgccug ccgcauucaa guuuuucgau aaaaucgucg acagaaagcg guacacguca     4020

acaaaagagg ugcuuaacag cacccugaua caccagagua uuacuggcuu guaugaaaca     4080

aggaucgauc uaggaaagcu gggcgaggac uga                                  4113


<210>  3134
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3134
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

guuacagaug acuauaaggu gccugcuaaa aagaugagag uauucgguga uacugacagg      120

ucucacauaa agaagaaucu ccuuggcacg cuguuguuug augacgggaa caccgcagag      180

ucacggcgcu uaaagcgaac agcccguaga agguacacuc ggcgcagaaa uaggauccug      240

uaucuacagg aaauuuucac cgagaguaug aacgaaaucg augagagcuu uuuccaucgg      300

cucgacgauu ccuuucuggu gccagaagac aaacgcggau cuaaguaccc gauuuucgcg      360

acacuucaag aggagaaaga auaucacaag caguuuccca ccaucuacca ucugcgaaaa      420

caguuggcug auucgaagga gaagucagac gucagacuca uauaucuggc ccuggcacac      480

augaucaaau acaggggcca uuuccucuac gaagagacuu uugauauuaa gaauaacgac      540

auccagaaaa uuuucaauga guuuaucaac auauaugaca auacauucga agguagcagu      600

uuauccgggc aaaacgccca gguggaggcu auuuuuaccg auaagaucuc uaaaagcgca      660

aagcgggaac guguucugaa acuuuucccu gacgagaagu ccacgggauu guucucagaa      720

uuucugaagc uaauuguggg caaucaggcc gauuucaaaa agcacuuuga ccucgaggag      780

aaagcuccac ugcaguucag uaaggauacu uacgacgaag aacuugaaaa ccuguugggc      840

caaaucgggg augacuuugc cgaucucuuc cugaucgcga aaaaguuaua ugacgcaauu      900

cugcucagcg gaauacuuac cgucacagau cccucuacua aggccccucu guccgcuuca      960

augaucgagc gcuacgaaaa ucaccagaaa gacuuggccg cacuaaagca guuuauuaaa     1020

aacaaucugc cagagaagua uguggaggua uucagcgacc agaguaaaga ugguuacgcu     1080

ggcuauaucg acgggaagac cacacaagaa gccuuuuaca aguacauuaa aaaccuccug     1140

ucuaaguucg agggagcgga uuauuuucuu gacaaaauag aaagagagga uuuccugagg     1200

aagcagcgga ccuuugacaa cggcuccauc ccccaucaga uccacuugca agaaaugaau     1260

gccauucucc gacgccaggg ugagcauuac ccuuuccuga aagagaacag agaaaagauc     1320

gagaagauuu uaacguucag gauaccauau uacguugggc cgcuggcacg ggauaaucgc     1380

gacuuuagcu ggcuaacuag aaacucggau gaacccauca ggccuuggaa uuucgaggag     1440

guggucgaca aagcucguuc ugccgaagau uuuauucacc ggaugacaaa cuaugaccuc     1500

uaccugccag aggaaaaggu gcuucccaaa cacuccuugc uguaugagac cuucgcugug     1560

uacaaugaac ucacuaaggu caaauuuauc gccgagggac ugcgagauua ccaguuccuu     1620

gacucaggcc agaagaaaca aaucguuaac cagcuguuua aggagaagag aaaagugaca     1680

gaaaaggaca uuauacauua uuugcacaau guagauggcu acgacgggau cgagcucaaa     1740

ggaauugaaa agcaguucaa cgcaaaucug aguaccuauc augauuuacu gaaaaucacg     1800

aaggacaagg aguuuaugga ugacccuaaa aacgaagaga uucuagagaa uaucgugcac     1860

acccucacaa uauucgaaga uagggagaug aucaagcagc gccuggccca auacgacacu     1920

cuuuuugaug aaaaagucau uaaggcuuug acccggagac auuauacagg uuggggcaaa     1980

cugagcgcga agcucaucaa cgggauuagg gacaagcagu ccggaaaaac uauccuggau     2040

uacuuaauag acgaugacaa gauuaaucgc aacuucaugc agcugaucaa cgacgauggc     2100

cuuucuuuua aagagauuau ccagaaggca caagugguug guaaaaccga cgaugugaag     2160

caggucgugc aggaauugcc agggagcccc gccauaaaga aaggcauccu ccagucaauu     2220

aagaucguag acgagcuggu uaaagugaug ggauaugcuc uagagaguau ugucaucgaa     2280

auggcccgag agaaucaaac aaccgcacgg ggcaagaaaa acucccagca gcguuacaag     2340

agaauagaag augcccugaa aaaucucgcu ccggggcugg acagcaacau ucuuaaggag     2400

aauccuacug auaacaucca auugaagaau gacaggcugu ucuuauacua ucuccagaac     2460

ggaaaagaua uguacacggg uaagccccug gacaucaauc agcuuucuuc auaugauauu     2520

gaccacauca uuccacaggc cuucauaaaa gacgauagua ucgacaaccg cgugcugaca     2580

agcuccaagg auaaucgggg caaaucugac aacgucccuu cguuggaggu gguucaaaag     2640

agaaaggcau uuuggcagca gcuccuggau agcaaacuaa uuuccgaaag gaaguucaau     2700

aaccugacca aagcggagcg agggggacuc gacgaacgcg auaagguggg cuuuauccgg     2760

agacagcugg uagagacuca acagauuacc aaaaaugucg cucagauccu ugacgccagg     2820

uucaacacag aagugaagga gaagaaccag aaaauacgua ccguuaagau cauuacuuug     2880

aaaucaaauc uggugaguaa cuuucgcaag gaguucgguu uauacaaagu ccgggaaauc     2940

aauaacuauc accaugcaca cgaugccuac cucaaugcug ugguagccaa ggcaauucug     3000

aagaaauauc ccaaacuuga gccagaauuu guguacgggg acuaccaaaa auaugaucug     3060

aagaaauaca ucucuagauu caagcccagc aaggagauag aaaaagcuac agagaaguac     3120

uuuuucuauu ccaaccuacu caacuuuuuc aaagaggaag uucuguaugc cgacggcacg     3180

auuaggaagc gagagaauau cgaauacuca aaagauaccg gagagaucgc gugggacaag     3240

gaaaaagacu ucgccacuau uaagaagguc uugagcuauc cucaggugaa uauagugaaa     3300

aagcgggagg uccagacagg cggguuuucu aaagagucca uccugccaaa gggaaacagu     3360

gauaaauuaa uuccgcgcaa gaccaaggac auccucuggg auacaacuaa auacgguggc     3420

uucgacagcc ccguuauugc uuacucuauc cugcuuauag cagauaucga aaaggggaaa     3480

gccaagaaac ugaagaccgu gaagacguug gugggaauua ccaucaugga gaaagcugcc     3540

uuugaagaga auccuauuac auuccucgag aacaagggcu aucauaaugu aagaaaagaa     3600

aacauccugu gccuaccaaa guacucccug uuugagcuug aaaaugguag gcggcgacuc     3660

cuggcaucag cgaaagaguu gcaaaagggg aacgaaauag uccugcccgu guguuuaacu     3720

acccuccugu aucacaguaa gaaccuucac aaacuggacg agccugagca uuuggaauac     3780

auucagaagc acagaaauga guucaaagau cuccugaacc uaguuagcga auuuucgcag     3840

aaguauaucc uggccgaggc uaaucuugag aaaaucaagg accucuacgc cgauaacgag     3900

caggcagaca uugagauccu ggcuaauucc uucauaaacu uguuaacauu uacugcccug     3960

ggcgcaccag ccgcuuucaa auuuuucgga aaggaugugg accgcaagag guauaccacg     4020

gucucugaaa uucucaaugc gacacugauc caucaaagca uuaccggccu uuacgagacu     4080

cguaucgauc ugucaaaauu gggggaagag uga                                  4113


<210>  3135
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3135
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

auaacagaug acuauaaggu uccugcuaaa aagaugaaag ugcucgguaa uacugauaag      120

aacuacauca agaaaaaucu ucugggcgca uuguuauucg acucugggaa cacggccgag      180

gauagaaggc ugaagcggac cgcgcgccga cguuauacaa gaaggcggaa ucgccuacuc      240

uaccugcagg acauuuucuc agaugaaaug aacaaaguag acgagaguuu cuuucacaga      300

cuugaagaua gcuuccuggu guccgaggac aagaggggag aucaucaccc aaucuuuggc      360

aaugucgugg acgaaguuaa auaucaugag caauucccga cuauuuacca cuugcggaag      420

uaucucgcug auacccccga gaaggccgac cugcgccugg uguaccucgc auuagcccau      480

augaucaaau uucgagguca cuuccugaua gacgauccua aacuugacgu caaaaacaca      540

gauucucagg aauuguuuuc gcaguuccug accgcuuacg acgugacuca ggaggauuca      600

caccuagcag acguaaagau caauauuagc gauaucauua cagaaaagau gaacaagagu      660

agaaaaguug aggcccuccu guuucauuuc ccaucccaaa agaagaauag cuuuuucggg      720

aaccuugugu cucuggucuu cggauugcuc cccaauuuua aaaccaacuu caaucuggcu      780

gaggacgcca aguuacagcu guccaaagau ucauaugacg aagaucucga gaaccuucug      840

ggccagaucg gcgacgaaua cacggacgug uuuguggcgg caaggaaguu guaugauagu      900

auacuacuga gcgggauucu cacugucaac gaccugucua ccaaagccau ccuuuccgcu      960

ucaaugguuc ggcguuacga ggaacacaau aaggaucugg ccaaguugaa agcauucgug     1020

aaggcuaacu uuacacauga cguauucaaa gagguguuua gcgaugccag uaagaaugga     1080

uaugcggguu acauugaggg caaaacuucu caggaagacu ucuauaagua cgucaagaac     1140

cucaucggga aaguggaugg auccgccgac uuuaucgaga agauugaucg cgaagacuuc     1200

cugagaaaac aaaggaccuu ugauaauggc agcauaccuc accagguuca cuuagcagag     1260

augaaggcua uccugcggcg acagggugaa uacuauccau uccuaaaaga gcaugccgag     1320

aagauucagc aaauccucac auuucgcaua cccuacuaug uggggccucu ggacagacac     1380

ggacauucgg auuucgcuug ggccucuuuc aacuccgaag agaaggucac cccauggaau     1440

uuugaagaga uuaucgacaa aaacaaguca gcacaggccu ucaucgaaag gaugacgaau     1500

caggaccuuu acuugccgaa cgagaaagug cugcccaagc acaguccucu cuaugagaaa     1560

uacacuguau auaaugaacu gacaaagauu aaauacguua ccgagacugg cacagcuaag     1620

uuuuucgaug cgaaccuuaa gcaagaaauc uuugacggcc uguucaaaaa ggagcggaaa     1680

gugaccaagc gcaaauugcu cacguuucug gagaagaauu ucgaugaauu uagaauuguc     1740

gacauacagg gguuagauaa ggagaccgaa acauucaacg caagcuacgg aaucuaucag     1800

gaccugcuaa aaauuaucaa ggauaaagcc uuuauggacg auuccgagaa ugcugacauc     1860

cucgaagaua uugugcugac ucuuaccuug uucgaggaca gggagaugau aaagcagcgu     1920

cuggccaaau acgcagaucu cuuugacaag aaggucaucg acaaccuggc ccggcgaaga     1980

uauacagguu ggggcagguu aucugcuaaa cugcuuaacg ggauucgcga uaagcggagc     2040

agaaaaacua ucauggacua cuugguggau gacggaaggu caaaucgcaa ccucaugcaa     2100

cugauuaccg augacaaucu aacauucaag gaugaaaucg ccaaagcaca guauagugac     2160

aacuccgaug accugcauca gguugugcag gaucucgcgg gcagcccagc uauaaagaag     2220

gguauccugc aaucuguaaa aauugucgac gagcuuguga agguuauggg guacgaaccc     2280

gagcagaucg uggucgaaau ggcccgagag aaucagacca cugcaaaagg ccggcguaac     2340

ucacagcaaa gauauaagag guuggaggaa gccauuaaaa aucuggaccg cgauuuaaac     2400

uacaagaucc ucaaagagca cccuacggac aaucaggcuc ugcagaacga ucggcuuuuc     2460

cuguacuauu ugcagaaugg aagagacaug uacacaggcg aaagucucga uauaaacagg     2520

cugagcgacu augauauuga ccacaucauu ccacaagccu uuaucaagga uaauuccaua     2580

gacaaccgag ugcuaguauc uucggcaaag aaucgcggga aaagcgauga cgugcccucc     2640

gaggauguug ucaaccggau gagaccguuc uggaacaagc ugcucaauug cggacugauc     2700

ucacagagga aauacaguaa ccuuaccaag aaagaauuga cuccuaauga caaggcuggu     2760

uuuauuaagc gucagcuggu ggagacccgc caaaucacaa aacauguggc ccggauuuua     2820

gacgagcacu ucaacaagga auuugaugag gacaaaaaga gaaucaggaa agucaagguu     2880

gugacccuca agucuaaucu ggugagcaac uuccgaaaag aauuugagcu uuauaaggua     2940

cgggagauaa augauuacca ucacgcgcac gacgccuauc ugaacgcugu cguggcaaaa     3000

gcccuacuca ugaaguaccc caaacuggaa ccagaguucg uuuacggcga auauccuaag     3060

uacaauuccu aucgcgagag aaagacugcu acacagagga uguuuuucua cucaaacauu     3120

augaauaugu uuaaaagcaa ggugaaauug gccgaugaac ggaucaucga gcgacccaug     3180

auugagguca acgacgaaac gggggagauc gcaugggaua agaccaaaca uauugcgacu     3240

gugaagaagg uucugucuua uccacagaua aauaucguga aaaagguaga ggaacagaca     3300

ggaggcuuaa ccaacgaaac aauuuacccc agagguuccu ucgacaaacu ccugaaucgc     3360

aagaacacuc uugaggccag uaaauauggg ggcuucggaa gcccuaccgu cgcuuacugu     3420

gugcuggugg ucgccgauuc uaagaacgag aauggcaaag uuaagaaguu gaaacucggg     3480

uccgaacugc uaggaaucac gauucuggag aagaacgacu uugaaaauaa cucacuugau     3540

uuccucagua ggaaagguua ccaaggcgug caggagaaga aaaucauaaa gcugccaaag     3600

uauagcuugu uugaauuuca ggacgggucg cgucggaugc uguccucugu aauggcaaau     3660

gauaacaaaa gaggagacuu acagaaggcu aaugagauga uccucccggc cuaccuggug     3720

aaacuucugu aucacgcaaa gcauauuaac agcgucggcg agcccgccca caaaaauuac     3780

aucaacgaac auugcucaga guuucaagac uugcucaauu auauuaggca guucgcugau     3840

aaguuugugg acguugauaa gaaccugagu aaaauccgcu cccuaucuaa gagcauggcg     3900

gaauacuccg ugucagagau auguagcagu uucgucaauc ugcuuacccu cacagcccug     3960

ggugcuccug cagacuuuaa auucuuuggg acuaccauuc cacgaaagag auauggcucu     4020

ccccagucca ucuugagcgc cacauuaauc caccagucga uuacuggacu guacgagacc     4080

cggauagauc ucucaaaacu gggcgaagac uga                                  4113


<210>  3136
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3136
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acuaugcaag aggaaaagua cuaucaugag aaauucccga ccaucuacca ccuucgcaag      420

gaacuggcag acaagaaaga gaaggccgau uugcgacuca uuuaccuggc ucuggcacau      480

aucauuaaau uuagagguca cuuccucauc gaggacgaua gguuugacgu gcggaacaca      540

gacauacaga agcaguauca ggccuucuua gaaauuuuug auaccacuuu cgagaauaac      600

gaccugcuuu cucaaaaugu cgauguggaa gcuaucuuga cagacaaaau uucgaaguca      660

gccaaaaagg aucguauccu ggcgcgcuac cccaaccaga aaagcaccgg gaucuuugca      720

gaguuccuaa aacucauugu uggaaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacgc cucuucaguu cgcuaaagac aguuaugaug aggaccugga gaauuugcuc      840

ggccaaauag gcgaugaauu ugccgaccug uucuccgcag cuaagaaguu auacgacucu      900

gugcugcuca gcgggauccu uacugucacc gaucugucca caaaagcccc auugucagcg      960

aguaugauuc agagauauga cgagcacagg gaagaucuaa agcagcugaa acaguuugug     1020

aaggccagcc uccccgagaa auaccaagaa aucuucgcag acucuuccaa ggauggauau     1080

gcugguuaca uagagggcaa gacuaaccag gaggccuuuu acaaauaucu gucaaaguug     1140

cugaccaaac aggaagggag cgaguacuug cucgaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaac gaacauuuga uaacggaagu aucccucacc agguacauuu aaccgaacug     1260

cgcgcuauca uuagaaggca gucugaguau uacccauucc uaaaggagaa ccucgaccgg     1320

aucgaaaaga uucugacguu ucgcauaccc uauuacguug gcccucuugc cagagagaag     1380

uccgauuucg cauggaugac uaggaaaaca gacgauagca uccguccaug gaauuuugaa     1440

gacuuggugg auaaggagaa aucggccgag gcuuucauuc accggaugac caacaaugac     1500

cuguaucucc cggaagagaa gguccugccc aaacauucuc uuaucuacga aaaguucacu     1560

guguacaacg agcugacaaa ggugcgauuu uuggcggaag guuucaaaga cuuucaguuc     1620

cucaauagaa agcaaaaaga gaccaucuuu aacucccugu ucaaggagaa aaggaagguc     1680

acggaaaagg auauuauauc auuuuuaaau aaaguugacg gguaugaggg aaucgcaauu     1740

aagggcaucg aaaaacaguu caacgccagu cugagcaccu accacgaucu aaagaaaauu     1800

cucggcaagg acuuucugga uaauacagac aacgagcuua ucuuggaaga uauagugcag     1860

acucugaccc ucuucgagga ccgcgagaug auccggaaga gacuggauau uuauaaagac     1920

uuuuucacag aaucccaguu aaagaaacug uacaggcgcc acuauacugg guggggacga     1980

cuuucugcua aguugaucaa ugguauucgg aacaaagaga aucaaaagac cauccucgau     2040

uaccugauag acgauggcag cgccaaccgu aauuuuaugc agcuaauuaa ggacgcaggg     2100

cugucauuca aaccuaucau ugacaaggcc agaacaggaa gucauuccga uaaccucaaa     2160

gaaguagugg gcgagcuggc ugguagccca gccaucaaga aagggauacu ucagucuuug     2220

aagaucgucg acgaacuggu gaaaguuaug ggcuaugagc ccgagcagau uguggucgaa     2280

auggcaaggg agaaccaaac cacugcgaag ggauuaucac gccuccggca gagacugacg     2340

acacuuaggg aaagucuggc uaaucuuaag agcaacaucc ucgaggagaa aaagccuaaa     2400

uacgugaagg aucagguaga aaaucaccau cuguccgacg aucgacuauu ccuguacuau     2460

cuccagaacg gcaaagacau guacaccgau gacgagcugg auauugacaa ucuuucucaa     2520

uaugauaucg accacauaau uccacaggcc uuuaucaagg acgauucgau cgacaaccgc     2580

guuuugguga gcuccgcaaa gaaucggggg aaaucagaug acguccccag uguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa cuguuagacg ccaagcucau gucucagaga     2700

aaguacgaua accugacuaa agcugagagg ggaggucuua ccagcgacga uaaggcccgu     2760

uuuauccaac gccagcuggu ugaaacacgg cagauuacca aacauguggc aagaauacua     2820

gacgagaggu ucaauaacga gcucgauucc aagggccgac ggauccgcaa aguaaagauu     2880

gucacucuga agucaaauuu ggugagcaac uuuagaaaag aguucggguu uuauaagauc     2940

agggaaguua auaacuacca ccacgcucau gacgccuauc ugaaugcggu ggucgccaaa     3000

gcuauuuuaa caaaguaccc gcagcucgaa ccugaguucg uguacggaga uuaucccaaa     3060

uacaacucuu auaagacgcg gaaguccgca accgaaaaac uguuuuucua caguaacauc     3120

augaauuucu ucaagacuaa aguaacacuu gccgacggca ccgugguugu caaggacgau     3180

auagagguga acaaugacac aggugagauc gugugggaua aaaagaaaca cuucgcuacu     3240

guccgaaagg uucugagcua uccacaagug aacauuguga agaaaaccga aauccagacc     3300

gggggcuuuu cuaaggaguc cauuuuggcc cauggaaauu cagacaaacu caucccuaga     3360

aagacgaaag auauauaccu ggaccccaag aaguauggcg gguucgauag uccaauugua     3420

gcauacagcg uccuaguggu ugcggacauc aaaaagggaa aagcccagaa gcugaaaaca     3480

gugacugaac uucucgguau caccauuaug gagcgcucga gguuugaaaa gaaccccucc     3540

gcuuuccugg agucuaaggg cuacuugaau auacgugaug acaaacugau gaucuuaccu     3600

aaguauagcc ucuuugagcu ggaaaacggg cggagaaggc uucuggccuc agcagaugag     3660

uugcagaaag gaaaugaacu cgcucugcca acacaauuca ugaaguuucu auaccuggcc     3720

agucgcuaua acgagcuuaa aggcaagccg gaggaaauug agcagaagca ggaauuuguc     3780

gugcagcacg uuuccuacuu ugacgauauc cuccaaauua ucaaugacuu cucuaaccga     3840

gugauacugg cagacgccaa uuuggagaaa aucaacaagu uauaucagga uaauaaagaa     3900

aacauucccg uagacgagcu ggcuaacaau aucauuaacc ucuuuacuuu caccagccug     3960

ggugcgccug ccgcauuuaa guucuucgau aaaaucgucg acagaaagcg guacacgucc     4020

acaaaagagg ugcuuaauuc aacccugaua caccagagca uuacuggguu guaugaaaca     4080

aggaucgauc uaggcaagcu gggagaggac uga                                  4113


<210>  3137
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3137
auggagaaga acaaagaaua cgugaucggc cuggacauug ggaccggaag cgucgguugg       60

uccgccauau cuacagauaa uuaucagcuc aucagacaaa agggcaaaaa ccuuauuggg      120

guuaggcugu ucaauucagc ugagacugca gaagaacggc gcggauaccg aacgacccgu      180

agaagguuga gucggcgcag auggaggaug agcuuacuga acgacaucuu ucggcccgaa      240

cuaaaugaga uugaugagaa cuuccucgcc cgccugaagu auuccugggu gucuccucuu      300

gaccagaaaa auccacagcu gaacgaacag ggcacaaaug cgucguugua cggugauauc      360

gaggcugacc aagccuuuca ccgagcauau ccgacuauau accaucucag aaagacccug      420

auggaagaua accagaagca cgaccugagg gagguauauc ucgccaucca ucacauugug      480

aaauaccggg ggcauuucuu aacacccgau uuugacgcua auucacagac caucgauauu      540

aagggacugu ucaaacagcu uggcgacguc agcggcagua ucgaguugca agcagaucag      600

acuuccacag ugauagccgc uuuucagucu auucugcuag acuucaccaa gacgccuacu      660

gaaagcgggc gucucgccgg acagcuguuu uccgaccaaa acaaagauaa gcaggcgaag      720

aaacagguug aggaacuuuu caagcuguug cgcgguggca aagggucacu cgcagacaug      780

uuucuguuaa ccgaucugga caaggugguc aaaaaucaac ucaaguucgc cuuugaugac      840

gcuguggaug agaaccuuaa ucugaucagu aacuugauua acgaggagca guacgagcua      900

cugcucaaua uccagaaacu gagaggaaca aucauucuua agcagauacu gggcgacgau      960

gaaacuguaa gcgacgccaa gguuaggucu uaugaggaac acgcacacca auugaaagag     1020

cucaaggcuc aggugcugac caaagauaac aaggaaaaau ucgaggaggc cuaccaguua     1080

auccagaagg gugacgcguc ccggauugac gggcugaaga aacuaaagac aaucauaaau     1140

caagaacaug aggcccuccg acuggauaaa uaugaagcag acaaacuuuu gaaacugauu     1200

gagcucgauc agcugcuucc aguccagcgc ucaaagagaa acggagaaau cccccaccag     1260

cugcaugagu ucgaguugaa gaaaaucauu gaaaagcaag gcagguacua uccuuuucuc     1320

cgggagaccu ucgaacgcga cggccaggag uuuaauaaaa ucgggcagcu ggugaacuuc     1380

agaauaccau acuauguggg acccuuaguc gauccuaggc uggcugguau acagagcggc     1440

gaccaaacga aucacuggau gacucagaag aaagccggga agaucacacc auggaacuac     1500

accgagcuaa uugauaucga caagagugcu gccgaauuua ucaaacguau gacuggaaca     1560

gauaccuacc ucguuggcga gccgucucug cacaaggcau cccuuauuua ucaggacuac     1620

aauguguugc aagaacugaa caaucuccgg uaugauggua aaccccugcc uguagacgcc     1680

aagcaggaga uauacagcaa cuuacaggug ucgcugaaaa augcuacgaa gaacaaaauc     1740

aauucuuucc uuauuuccga aggguauuug caggauggcg aggagcucac cggauuugcg     1800

ggcaaggaac ugaagaacaa acuaucaagu uuccauuacu uuaagaacau cuucgggcca     1860

gagcgaauga auacacuggg aacugacaac cucgaaaaga uuaccgagcu gcaaacaguc     1920

uuugaagaca gacacaugcu uaagaggcag uugcagaaac uggguuucuu agaugacgau     1980

gagugcgaga agcucgcagg caagcauuuu acuggguggg gaaaucugag caaaaagcug     2040

cuuaacaccc ccgccaucau agacccugaa aucaaugaga acaaaauuuc cuugaucgau     2100

gcucucuaua agggccagcg caaucugucu uucuacguga acaauaugga cgauaaauac     2160

caauuccggg acuggauuga aagcgagaac cagucacagc agguugaaau cagucuagau     2220

gagaagauaa agaaucuggc cgguucccca gcaauuaaaa gagggaucac acaaagcaug     2280

gccgugauuc aggacaucau aagggcuaac aauggcaucu cucccaagcg cauuuaucuc     2340

gaguuugccc gagaaucaca gaacaguuac caaccgaaua gccggaucaa acagauugag     2400

aagaucuaug aucagaacaa aaaggaacug augaaggucu acccugacgu guucaaagau     2460

guacagggag uucuuaacaa uguggacgag aacaauuccu ugucugagaa ggcauuucug     2520

uauuucaugc aauuaggcaa agacauguac accggggaag cgcucgauuu ugacaagcug     2580

uauacuuacg auauagacca cauuaucccc caggcuauga cgaaagauaa cucgugggac     2640

aauaaggucc uugugacagc caucaaaaac cgugaaaaga gcaaucaggu ccuggauaac     2700

gcaauucagg ccaagaugca uaauuucugg cuacaacuca aaaaggugaa ccuguugucc     2760

cagagaaaau acgacacccu gaugaagacu aauuuuucaa aaggagauga aaagaaguuc     2820

aucgcuagga guuuaguuga gacccgccag auuauuaaaa acguggccga acucaucaag     2880

ucugaguuug accacguaga ggcaauucca auccggcagg aacugacaag cgauaugaga     2940

caacuuaugg acuauaaaaa gaauaggcug gucaacgacu accaccaugc ucacgaugcc     3000

uugcucauug cgaccguggg ugaguucaug cagaaacgag gcuuuaugca ggggaugaau     3060

ccuucccaga acguuuauca uggauacacu caauauguga agaaguggcu gucaauggcc     3120

aaaaaggcuc gcagcgcaga aaaacccgag cggaucuucc cacuacaguu ugucgugggc     3180

ucuaugagaa uggccguaag ggacggucag guggauaagg uuacacaccc cgcuacgggg     3240

gaaauauguu ggaccgccga caacgaggca uaccugcgua aaguccuugg cuauaagaau     3300

auccucguga cucgcaaggu gaaaaacgcg ggaaagcugu accaacgggu cacaguuaaa     3360

ccuaagggcu ccggggaggc cccagugaau caccagaaaa gugauauuaa caucuacgga     3420

ggcgugacca gcgaacaggc ugccgaguug guacugauua gaaucaaggc aaagaauaac     3480

gcuaaaaaua uggucauaaa gauuccgaca gccaucgcaa accagguggc caauaaaacu     3540

accacguaug acgcuuacgu uaggucuuua aacaucccca auuaugcggg uauuauacuc     3600

gaucaaaucc cucuggggca gcuuguguuc gacgaacgaa ccuccuucua ccuggccuca     3660

gcagagauua agcauaacgc ucggcaguug ugggucaauc agcacaucaa caaugugcuc     3720

acuaaaaaga acauuaauga ugaggagcgc gacaacaucc ugaaccuacu gacaaaugag     3780

aaaaaccuug aaagauuuaa gcuccuggag ccagauuuga agaggguuaa agccgccaag     3840

caacaguggg cuaguugcga cgaucaggaa cagaugcaaa uauuacggau cauuaucaau     3900

aaaaugcaug acgacgcaag cccccagauu ccuggaaaga ucgauccauu caaaaacuau     3960

cuggacaagc agggcaaggg uuggcgagau cucagaacca aaaagggcau gaaacugucg     4020

gacaaugccg aguuugugua ccaauccccc acagggcuuu aucaccgcaa gauaucuauu     4080

aacgagcugu ugaaacagaa gcagcuagaa uga                                  4113


<210>  3138
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3138
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acuaugcaag aggaaaagua cuaucaugag aaauucccga ccaucuacca ccuucgcaag      420

gaacuggcag acaagaaaga gaaggccgau uugcgacuca uuuaccuggc ucuggcacau      480

aucauuaaau uuagagguca cuuccucauc gaggacgaua gguuugacgu gcggaacaca      540

gacauacaga agcaguauca ggccuucuua gaaauuuuug auaccacuuu cgagaauaac      600

gaccugcuuu cucaaaaugu cgauguggaa gcuaucuuga cagacaaaau uucgaaguca      660

gccaaaaagg aucguauccu ggcgcgcuac cccaaccaaa agagcaccgg gaucuuugca      720

gaguuccuaa aacucauugu uggaaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacgc cucuucaguu cgcuaaagac aguuaugaug aggaccugga gaauuugcuc      840

ggccagauag gcgaugaauu ugccgaccug uucuccgcag cuaagaaguu auacgacucu      900

gugcugcuca gcgggauccu uacugucacc gaucugucca caaaagcccc auugucagcg      960

aguaugauuc agagauauga cgagcacagg gaagaucuaa agcagcugaa acaguuugug     1020

aaggccagcc uccccgagaa auaccaagaa aucuucgcag acucuuccaa ggauggauau     1080

gcugguuaca uagagggcaa gacuaaccag gaggccuuuu acaaauaucu gucaaagcug     1140

cugaccaaac aggaagggag cgaguacuug cucgaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaac gaacauuuga uaacggaagu aucccucacc agguacauuu aaccgaacug     1260

cgcgcuauca uuagaaggca gucugaguau uacccauucc uaaaggagaa ccucgaccgg     1320

aucgaaaaga uucugacguu ucgcauaccc uauuacguug gcccucuugc cagagagaag     1380

uccgauuucg cauggaugac uaggaaaaca gacgauagca uccguccaug gaauuuugaa     1440

gacuuggugg auaaggagaa aucggccgag gcuuucauuc accggaugac caacaaugac     1500

cuguaucucc cggaagagaa gguccugccc aaacauucuc uuaucuacga aaaguucacu     1560

guguacaacg agcugacaaa ggugcgauuu uuggcggaag guuucaaaga cuuucaguuc     1620

cucaauagaa agcaaaaaga gaccaucuuu aacucccugu ucaaggagaa aaggaagguc     1680

acggaaaagg auauuauauc auuuuuaaau aaaguugacg gguaugaggg aaucgcaauu     1740

aagggcaucg aaaaacaguu caacgccagu cugagcaccu accacgaucu aaagaaaauu     1800

cucggcaagg acuuucugga uaauacagac aacgagcuua ucuuggaaga uauagugcag     1860

acucugaccc ucuucgagga ccgcgagaug auccggaaga gacuggauau uuauaaagac     1920

uuuuucacag aaucccaguu aaagaaacug uacaggcgcc acuauacugg guggggacga     1980

cuuucugcua aguugaucaa ugguauucgg aacaaagaga aucaaaagac cauccucgau     2040

uaccugauag acgauggcag cgccaaccgu aauuuuaugc agcuaauuaa ggacgcaggg     2100

cugucauuca aaccuaucau ugacaaggcc agaacaggaa gucaucucga uaaccugaaa     2160

gaaguagugg gcgagcuugc ugguucccca gccaucaaga aagggauauu gcagagccug     2220

aagaucgucg acgaauuagu gaaaguuaug ggcuaugagc ccgagcagau uguggucgaa     2280

auggcaaggg agaaccaaac cacugcgaag ggacucucuc gcucacggca gagacugacg     2340

acacuuaggg aaagucuggc uaaucuuaag agcaacaucc ucgaggagaa aaagccuaaa     2400

uacgugaagg aucagguaga aaaucaccau cuguccgacg aucgacuauu ccuguacuau     2460

cuccagaacg gcaaagacau guacaccgau gacgagcugg auauugacaa ucuuucucaa     2520

uaugauaucg accacauaau uccacaggcc uuuaucaagg acgauucgau cgacaaccgc     2580

guuuugguga gcuccgcaaa gaaucggggg aaaucagaug acguccccag ucuggaaauu     2640

gugaaggauu gcaaagucuu cuggaagaaa uuacucgacg ccaagcugau gucucagaga     2700

aaguacgaua accuuacuaa agcugagagg ggaggucuga ccagcgacga uaaggcccgu     2760

uuuauccaac gccagcuagu ggaaacacgg cagauuacca aacauguugc aagaauacuc     2820

gacgagaggu ucaauaacga gcuggauucc aagggccgac ggauccgcaa agugaagauu     2880

guaacuuuga agucaaaucu ggucagcaac uuuagaaaag aauuuggguu uuauaagauc     2940

agggagguga auaacuacca ccacgcucau gacgccuauu uaaaugcggu uguggccaaa     3000

gcuauucuca caaaguaccc gcagcuggaa ccugaguucg ucuacggaga uuaucccaaa     3060

uacaacucuu auaagacgcg gaaguccgca accgaaaaau uguuuuucua caguaacauc     3120

augaauuucu ucaagacuaa agugacacug gccgacggca ccguaguggu uaaggacgau     3180

auagagguca acaaugacac aggugagauc gugugggaua aaaagaaaca cuucgcuacu     3240

gugcgaaagg ucuugagcua uccacaaguu aacauuguga agaaaaccga agugcagacc     3300

gggggcuuuu cuaaggaguc cauccucgcc cauggaaauu cagacaaacu gauuccuaga     3360

aagacgaaag auaucuaccu agaccccaag aaguauggcg gguucgauag uccaauagua     3420

gcauacagcg uccugguggu ugcggacauu aaaaagggaa aagcccagaa acuuaaaaca     3480

gugacugaac uccuggguau caccaucaug gagcgcucga gguuugaaaa gaaccccucc     3540

gcuuucuugg agucuaaggg cuaccugaau auucguacag auaaauuaau aauccucccu     3600

aaguauagcc uguuugagcu ugaaaacggg cggagaaggc uguuggccuc agcaggagag     3660

cuccagaaag gcaaugaacu ggcucuacca acucaauaca ugaaguuccu guaucuugcc     3720

agucgcuaca acgagcucaa agguaagccg gaggaaauug agcagaagca ggaauuuguc     3780

gugcagcacg uuuccuauuu cgacgauauc cugcaaauua ucaaugacuu uucuaaccga     3840

gugauauugg cagaugccaa uuuagagaaa aucaacaagc uguaccagga caauaaagaa     3900

aacauucccg uagacgagcu cgcuaacaau aucauuaacc uguucaccuu uacgagccuu     3960

ggggcgccug ccgcauucaa guuuuucgau aaaaucgucg acagaaagcg guauacaucc     4020

accaaagagg ugcuuaauuc aacuuugaua caccagagca uuacaggccu auacgaaacc     4080

aggaucgauc ugggaaagcu cggcgagggg uga                                  4113


<210>  3139
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3139
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaggu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acucuucaag aggaaaagga cuaccaugag aaauucucua ccaucuauca ucugcgcaag      420

gaauuggcag auaagaaaga gaaggccgac cuccgacuga uuuacaucgc ucuggcacac      480

auuaucaaau uuagagguca cuuccucaua gaggaugacu cguuugacgu gaggaacaca      540

gauauuucaa agcaguacca ggacuucuua gaaaucuuua auaccacuuu cgagaacaau      600

gaucugcuua gccagaacgu cgacguggaa gccauuuuga cagauaaaau caguaagucc      660

gcuaaaaagg accggauccu ggcccaauau ccgaaucaga agucuaccgg gauuuuugcg      720

gaguuccuaa aacucauagu uggaaaccag gcagauuuca agaaauacuu uaaucuggaa      780

gacaagacgc cccuucaguu cgccaaagau agcuaugacg aggaucugga gaacuugcuc      840

ggccaaaucg gcgacgaauu ugcugaccug uucuccgccg caaagaaguu auacgauuca      900

gugcugcuca gugggauucu uacugucauc gaccugagca ccaaagcucc uuugucugcc      960

uccaugauac agcguuauga ugagcaucgc gaagaccuaa agcagcugaa acaguuugug     1020

aaggcgucac ucccagagaa auaccaagaa auuuucgccg auagcaguaa ggacggauau     1080

gcagguuaca ucgagggcaa gacaaaucag gaggcuuuuu acaaauaucu gucuaaacuu     1140

cugacuaagc aggaagauuc cgagaacuuc uuggaaaaga ucaaaaacga ggacuuucuc     1200

agaaagcaaa ggaccuucga uaaugggagc auuccccacc agguacaccu gacagaauua     1260

aaggccauca uucggcgaca gucggaguac uauccuuuuc ugaaagagaa ccaggaccgc     1320

auagaaaaga uccuaaccuu caggauucca uacuauaucg gaccccucgc uagagagaaa     1380

ucugauuuug ccuggaugac gcggaagacu gacgauucca uccgcccuug gaauuucgaa     1440

gaccugguug acaaagagaa gucagcagag gccuucauuc auagaaugac aaacaaugau     1500

uuuuaccuuc cagaagagaa aguguugccg aagcacaguc ugauauauga aaaguucacc     1560

gucuacaacg agcucacuaa agugagguac aagaaugaac aaggcgagac auacuuuuuc     1620

gacagcaaca ucaaacagga gauuuuugau gguguguuca aggaacaucg uaaagucucc     1680

aagaagaaac ugcuugacuu ucuggcuaag gaguaugaag aguuccggau cguugaugug     1740

auuggguugg acaaagaaaa uaaggcguuu aacgcaucuc ucggaaccua ucacgaucug     1800

gagaaaaucu uagacaagga uuuccuggac aaucccgaua acgagagcau acuagaagac     1860

aucguacaga cgcucacccu guuugaggau cgagaaauga uuaagaaaag acuugagaau     1920

uacaaggacu uguucacaga aucacagcug aaaaagcucu auaggcgcca cuacacuggc     1980

uggggccggc ugagugccaa auuaaucaac gggauuagag acaaggaguc ccaaaagacc     2040

auccuggauu aucuuauaga cgauggaagg agcaaucgca acuuuaugca guugauuaac     2100

gacgaugguc ucucuuucaa aucaaucauu aguaaggcuc aggccggcag ccauuccgac     2160

aaucugaaag agguggucgg ggaacuagca ggaucuccug ccaucaagaa aggcauacug     2220

cagucgcuca agaucgugga ugagcugguu aaagugaugg guuacgaacc agagcaaauu     2280

gucguggaga uggcucgaga aaaccagaca acuaaucagg ggcggcguaa cagcagacag     2340

agguacaaac uuuuggacga uggcguaaag aaucuggccu ccgacuuaaa cggaaauauc     2400

cucaaggagu aucccaccga uaaccaagca cugcagaaug aacgccuuuu ccuguacuau     2460

uugcagaacg gccgggacau guacacaggg gaggcgcucg acauugauga ccugucacaa     2520

uaugauaucg accacauaau uccucaggcu uuuaucaagg augacaguau cgauaauaga     2580

guucuagugu cuagcgccaa aaacagggga aaguccgacg augucccauc acuggaaauu     2640

gugaaagacu gcaaggucuu cuggaagaaa cuccuggaug caaaacuuau gagccagcga     2700

aaguacgaca auuugaccaa ggccgagcgc gguggccuga cuucugauga caaagcucgg     2760

uuuauccaga gacaauuagu ggagacgagg cagauuacaa agcauguugc ccguauacuc     2820

gacgaacgcu ucaacaauga gcuggauucc aaggggcgga gaaucaggaa agugaagauu     2880

guaacccuua aaaguaaccu ggucagcaac uuucgaaagg aguucggauu uuauaaaauc     2940

cgggaaguga auaacuacca ccaugcacac gacgcuuacc uaaaugccgu uguggcgaag     3000

gccauucuca cuaaguaucc ccagcuggaa ccggaguucg ucuacggcga uuauccuaaa     3060

uacaacucuu auaagacccg caaauccgcu acagagaagu uguuuuucua cucaaauauc     3120

augaacuuuu ucaaaaccaa ggugacucug gcagacggua caguaguggu uaaggaugac     3180

auagaaguca auaacgauac gggggagauc gugugggaca aaaagaaaca cuucgccacc     3240

gugagaaagg ucuuaaguua uccccagaau aacauuguua aaaagacuga aauccaaaca     3300

ggcggauuua gcaaagaguc gauucucgcu cauggcaauu ccgauaagcu gaucccaagg     3360

aagaccaaag acauauaccu ugauccuaag aaauacgggg gauucgacuc ucccauugug     3420

gccuauagcg ugcugguagu cgcagauauc aagaaaggua aggcgcagaa guugaaaaca     3480

gugacugaac uccugggcau caccauuaug gagcggucac gauuugagaa gaacccaagu     3540

gccuuccuag aauccaaagg guaccugaau auaagagcug acaaacuuau cauucucccc     3600

aaguauucuc uguuugaguu ggaaaacgga cgcaggcguc uguuagccag cgcaggcgag     3660

cuccagaagg guaaugagcu ggcccuuccu acgcaguuca ugaaguuucu guacuuggcu     3720

ucccgguaua acgaaucaaa agggaagcca gaggaaaucg agaaaaagca agaguucguu     3780

aaccagcacg ugagcuacuu ugacgauauu cuccagcuga ucaaugacuu caguaaaaga     3840

gucauacuag cagaugccaa ccuggaaaag aucaauaaac uuuaucaaga caacaaggag     3900

aauauuucug uggaugaacu cgcuaacaau aucauuaacc uguuuaccuu cacauccuug     3960

ggcgcgccgg ccgcauuuaa guucuucgac aaaaucguug auaggaagcg cuacacuagc     4020

accaaagagg uguuaaauuc gacacugaua caucagucaa uuacuggacu cuacgaaacc     4080

cgaaucgacc ugggcaaacu uggggaggau uga                                  4113


<210>  3140
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3140
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acucuucaag aggaaaagga cuaccaugag aaauucucua ccaucuauca ucugcgcaag      420

gaauuggcag auaagaaaga gcgagccgac cucagacuga uuuacaucgc ucuggcacac      480

auuaucaagu uuagggguca cuuccucaua gaggaugacu cguuugacgu gcggaacaca      540

gauauuucaa aacaguacca ggacuucuua gaaaucuuua auaccacuuu cgagaacaau      600

gaucugcuua gccagaacgu cgacguggaa gccauuuuga cagauaagau caguaaaucc      660

gcuaagaaag accguauccu ggcccaauau ccgaaucaga agucuaccgg gauuuuugcg      720

gaguuccuaa agcucauagu uggaaaccag gcagauuuca aaaaguacuu uaaucuggaa      780

gacaaaacgc cccuucaguu cgccaaggau agcuaugacg aggaucugga gaacuugcuc      840

ggccaaaucg gcgacgaauu ugcugaccug uucuccgccg cuaaaaaguu auacgauuca      900

gugcugcuca gugggauucu uacugucauc gaccugagca ccaaggcucc uuugucugcc      960

uccaugauac agcgcuauga ugagcauaga gaagaccuaa aacagcugaa gcaguuugug     1020

aaagcgucac ucccagagaa guaccaagaa auuuucgccg auagcaguaa agacggauau     1080

gcagguuaca ucgagggcaa gacaaaucag gaggcuuuuu acaaguaucu gucuaaacuu     1140

cugacuaagc aggaagauuc cgagaacuuc uuggaaaaga ucaagaacga ggacuuucuc     1200

aggaaacaac ggaccuucga uaaugggagc auuccccacc agguacaccu gacagaauua     1260

aaggccauca uucgacgcca gucggaguac uauccuuuuc ugaaggagaa ccaggacaga     1320

auagagaaaa uccuaaccuu caggauucca uacuauaucg gaccccucgc ucgggaaaag     1380

ucugauuuug ccuggaugac gcgcaaaacu gacgauucca ucagaccuug gaauuucgaa     1440

gaccugguug acaaggagaa aucagcagag gccuucauuc auaggaugac aaacaaugau     1500

uuuuaccuuc cagaagagaa gguguugccg aaacacaguc ugauauauga aaaguucacc     1560

gucuacaacg agcucacuaa ggugcguuac aaaaaugaac aaggcgagac auauuucuuc     1620

gacagcaaca ucaagcagga gauuuuugau gguguguuca aagaacaucg gaaggucucc     1680

aaaaagaagc ugcuugacuu ucuggcuaaa gaguacgaag aguuccgaau cguugaugug     1740

auuggguugg acaaggaaaa uaaagcguuu aacgcaucuc ucggaaccua ucacgaucug     1800

gagaagaucu uagacaaaga uuuccuggac aaucccgaua acgagagcau acuagaagac     1860

aucguacaga cgcucacccu guuugaggau agagaaauga uuaagaagag gcuugagaau     1920

uacaaagacu uguucacaga aucacagcug aagaaacucu aucgccggca cuacacuggc     1980

uggggcagac ugagugccaa guuaaucaac gggauuaggg acaaagaguc ccaaaagacc     2040

auccuggauu aucuuauaga cgauggacgc agcaaucgaa acuuuaugca guugauuaac     2100

gacgaugguc ucucuuucaa gucaaucauu aguaaagcuc aggccggcag ccauuccgac     2160

aaucugaagg agguggucgg ggaacuagca ggaucuccug ccaucaaaaa gggcauacug     2220

cagucgcuca aaaucgugga ugagcugguu aaggugaugg guuacgaacc agagcaaauu     2280

gucguggaga uggcucggga aaaccagaca acuaaucagg ggcguagaaa cagcaggcag     2340

cgcuacaaac uuuuggacga uggcguaaag aaucuggccu ccgacuuaaa cggaaauauc     2400

cucaaggagu aucccaccga uaaccaagca cugcagaaug aacggcuuuu ccuguacuau     2460

uugcagaacg gcagagacau guacacaggg gaggcgcucg acauugauga ccugucacaa     2520

uaugauaucg accacauaau uccucaggcu uuuaucaaag augacaguau cgauaauagg     2580

guucuagugu cuagcgccaa gaaccgagga aaauccgacg augucccauc acuggaaauu     2640

gugaaggacu gcaaagucuu cuggaagaag cuccuggaug caaaacuuau gagccagcgc     2700

aaguacgaca auuugaccaa agccgagcgg gguggccuga cuucugauga caaggcuaga     2760

uuuauccaga ggcaauuagu ggagacgcgu cagauuacaa aacauguugc ccgcauacuc     2820

gacgaacggu ucaacaauga gcuggauucc aaggggagaa ggauccgaaa ggugaaaauu     2880

guaacccuua agaguaaccu ggucagcaac uuucggaaag aguucggauu uuauaagauc     2940

cgcgaaguga auaacuacca ccaugcacac gacgcuuacc uaaaugccgu uguggcgaaa     3000

gccauucuca cuaaguaucc ccagcuggaa ccggaguucg ucuacggcga uuauccuaag     3060

uacaacucuu auaaaaccag aaaguccgcu acagagaaau uguuuuucua cucaaauauc     3120

augaacuuuu ucaagaccaa agugacucug gcagacggua caguaguggu uaaggaugac     3180

auagaaguca auaacgauac gggggagauc gugugggaca agaaaaagca cuucgccacc     3240

gugaggaaag ucuuaaguua uccccagaau aacauuguua agaaaacuga aauccaaaca     3300

ggcggauuua gcaaggaguc gauucucgcu cauggcaauu ccgauaaacu gaucccacgg     3360

aagaccaagg acauauaccu ugauccuaaa aaguacgggg gauucgacuc ucccauugug     3420

gccuauagcg ugcugguagu cgcagauauc aaaaagggua aagcgcagaa guugaagaca     3480

gugacugaac uccugggcau caccauuaug gagcgaucaa gauuugagaa aaacccaagu     3540

gccuuccuag aauccaaggg guaccugaau auacgcgcug acaaacuuau cauucucccc     3600

aaguauucuc uguuugaguu ggaaaacgga aggcgucggc uguuagccag cgcaggcgag     3660

cuccagaaag guaaugagcu ggcacuuccu acgcaguuca ugaaguuucu guacuuggcc     3720

uccagauaua acgaaucaaa ggggaaacca gaggaaaucg agaagaaaca agaauuuguu     3780

aaccagcacg ugagcuacuu ugacgauauu cuccagcuga ucaaugacuu caguaagagg     3840

gucauacuag cagaugccaa ccuggagaaa aucaauaaac uuuaucaaga caacaaggag     3900

aauauuucug uggaugaacu cgcuaacaau aucauuaacc uguuuaccuu cacauccuug     3960

ggcgcgccgg ccgcauuuaa auucuucgac aagaucguug aucgcaaacg auacacuagc     4020

accaaggagg uguuaaauuc gacacugaua caucagucaa uuacuggacu cuacgaaacc     4080

agaaucgacc ugggcaaacu uggggaggau uga                                  4113


<210>  3141
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3141
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcauca      180

gauaggcggu uaaagcgcac cgcccgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ggaggaaaug aguaaagugg acgauagcuu cuuucacaga      300

cucgaggacu ccuuccuggu agaagaugac aagcggggcu cuaaauaucc aauauuugcu      360

acuaugcaag aggagaagga uuaccaugaa aaauucccga ccaucuauca ccuucgcaag      420

gagcuggccg acaagaaaga aaaggcagau uugcgacucu uuuaccuggc ccuggcucau      480

auuaucaaau ucagagguca cuuucucauu gaggacgacu cguucgaugu gaggaacaca      540

gacauccagc ggcaguacca ggcauuuuua gagauauucg auaccacuuu ugaaaauaac      600

caucugcuuu cacaaaauau ugacgucgag gggaucuuga cagauaagau uagcaaaagu      660

gccaagaaag accguauccu ggcucaguau cccaaccaga aguccaccgg aaucuucgcc      720

gaguuccuaa agcucauugu gggcaaucag gcggauuuua aaaagcacuu caaccuggaa      780

gacaaaacgc cucuucaauu ugcaaaggau ucuuacgacg aagaucugga gaauuugcuc      840

ggccagauag gggacgaguu cgccgaccug uuuagcguug cuaaaaaguu auaugauucc      900

gugcugcucu caggaauccu uacugucacc gaccugagua caaaggcccc auugagcgca      960

ucuaugauuc agcgcuacga ugaacacaga gaggaccuaa aacagcugaa gcaauucgug     1020

aaagcuagcc uccccgaaaa guaucaggag aucuuuacug auucaagcaa agacgguuac     1080

gccggcuaua uagaagggaa gaccaaccag ggagcguucu acaaguaccu gaguaaacuu     1140

cugacaaagc aagagggcuc ugaguauuuu cuugaaaaga uuaagaauga ggauuuccuc     1200

aggaaacagc ggaccuuuga caacgguucc aucccucauc agguacaccu gacggaauua     1260

aaggccauca uucgacgcca gagcgaguac uauccauucc ugaaggaaaa ccuagauaga     1320

aucgagaaaa uucucacuuu uaggauaccc uacuauguug ggccucuggc acgggagaag     1380

ucggacuucg cuuggaugac acgcaaaacc gaugacucua ucagaccaug gaauuucgag     1440

gaacuugugg auaaggaagc cuccgcugag gccuuuauuc auaggaugac uaacaaugac     1500

uuguaccugc cggaggaaaa aguccucccc aagcacucac ugaucuauga gaaauucaca     1560

guguacaacg aacuuaccaa ggugcguuac aagaaugagc aaggagaaac guacuuuuuc     1620

gacaguaaca ucaaacagga gauuuuugau ggcgucuuca aggagcaccg gaaaguuagc     1680

aagaaaaagc uguuggacuu ucucgcaaag gaauaugagg aguuccgaau aguggaugua     1740

aucggccugg acaaagaaaa uaaggccuuu aacgcuuccu uagggaccua ucaugaucug     1800

aaaaagauuc uagacaaaga uuuccucgac aauccugaua acgaaucuau ccuggaggac     1860

auugugcaga cacuuacuuu guuugaggau agagaaauga ucaagaagag gcuggagaau     1920

uacaaagacc ucuucaccga aagccagcug aagaaauuau aucgccggca cuacacagga     1980

ugggguagac ugucagcgaa acuuauaaac ggcaucaggg auaaagagag ucaaaagacu     2040

auuuuggacu aucucaucga cgaugggaag uccaaucgca acuuuaugca gcugauucau     2100

gacgauggac uauccuucaa aucuaucaua ucaaaggcac aggccggcag ucacagcgac     2160

aaucugaaag aagucguggg ugagcucgcu ggguccccag ccauuaagaa aggcauccug     2220

cagucucuua agauuguuga ugaguuggug aaagucaugg gauacgaacc cgagcaaauc     2280

gugguagaaa uggcacgaga gaaccagacc acaaaccagg gccggcguaa uucgagacag     2340

agguacaagc uguuagacga ugggguuaag aaccucgcca gcgaccugaa uggaaacaua     2400

cuuaaagagu auccuaccga uaaucaagcu cugcagaacg aacgcuuguu ccucuacuau     2460

cugcagaaug gucgggacau guacacuggc aaggcccuag auaucgacaa ccugucccaa     2520

uaugacauug aucauaucau uccacaggca uuuaucaaag acgauucaau agacaauaga     2580

gugcucguca guucugcgaa gaacaggggg aaaagcgaug acgugcccuc ccuggagauu     2640

gucaaggauu gcaagguguu cuggaaaaag uuguuggacg cuaaacugau gucacagcga     2700

aaguacgaua auuuaacgaa agccgaacgc ggaggccuca caagcgacga uaaggcacgg     2760

uuuauccaga gacaacuggu ugagaccagg cagaucacua agcacguggc ccguauucuu     2820

gacgaacgcu ucaacaauga gcuggauucu aaaggucgga gaaucaggaa gguaaaaauu     2880

gucacccuaa aguccaaccu cgugaguaau uuucgaaaag aguucggguu uuauaagaua     2940

cgggaaguua acaacuacca ccaugcucac gacgccuacc ugaaugcagu ggucgcuaag     3000

gccaucuuga caaaauaucc gcagcuggag ccugaguucg uguacggcga cuaucccaag     3060

uacaacagcu auaaaacccg caagucugcg acugaaaaau uauuuuucua cuccaauauu     3120

augaacuuuu ucaagacaaa gguaacgcuc gccgauggaa ccgugguugu caaagacgau     3180

aucgaaguga auaacgacac uggcgagauu gugugggaua agaaaaagca uuucgcuaca     3240

gucagaaaag uucugucaua uccacaggug aauaucguga agaaaaccga gauacaaaca     3300

gggggauuua guaaggaaag cauccuugca cacgguaacu cggacaagcu gauuccuagg     3360

aaaacuaagg auaucuacuu ggaccccaaa aaguacggcg gguucgauuc cccaauugua     3420

gccuauucug uccucguggu ugcugacauc aaaaagggaa aggcccagaa acugaagacc     3480

gugacggagc uacugggcau aaccauuaug gaacggagcc gauuugagaa aaaucccuca     3540

gcauuccuug aaaguaaggg uuaccucaac aucagagaug acaaacugau gaucuugccu     3600

aaguauuccc uguuugaguu agagaauggg cgcaggcguc uccuggcguc ugccggcgaa     3660

cuucagaagg gaaacgagcu ggcuuugcca acacaguuca ugaaauuucu cuaccuggcc     3720

agccgguaua augaacuaaa gggcaaaccg gaggagauug aacaaaagca ggaguucguc     3780

gugcagcacg uuuccuacuu ugaugacaua cugcaaauca uuaacgacuu cucaaauaga     3840

gugauccuug cagaugcuaa ccucgagaaa auuaacaagc uguaucagga caauaaggaa     3900

aacaucagcg uagaugaauu ggccaauaac auaaucaauu uauuuacuuu caccagucug     3960

ggggcacccg ccgcuuuuaa auucuucgac aagauugucg auaggaaacg cuacacaucu     4020

acuaaggagg ugcucaacuc cacccugauc caucagagca uuacgggacu uuacgagaca     4080

cgaaucgacc ugggcaaauu gggugaagau uga                                  4113


<210>  3142
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3142
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acuaugcaag aggaaaagua cuaucaugag aaauucccga ccaucuacca ccuucgcaag      420

gaacuggcag acaagaaaga gaaggccgau uugcgacuca uuuaccuggc ucuggcacau      480

aucauuaaau uuagagguca cuuccucauc gaggacgaua gguuugacgu gcggaacaca      540

gacauacaga agcaguauca ggccuucuua gaaauuuuug auaccacuuu cgagaauaac      600

gaccugcuuu cucaaaaugu cgauguggaa gcuaucuuga cagacaaaau uucgaaguca      660

gccaaaaagg aucguauccu ggcgcgcuac cccaaccaaa agagcaccgg gaucuuugca      720

gaguuccuaa aacucauugu uggaaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacgc cucuucaguu cgcuaaagac aguuaugaug aggaccugga gaauuugcuc      840

ggccagauag gcgaugaauu ugccgaccug uucuccgcag cuaagaaguu auacgacucu      900

gugcugcuca gcgggauccu uacugucacc gaucugucca caaaagcccc auugucagcg      960

aguaugauuc agcacuauga cgagcacaga gaagaucuaa agcagcugaa acaguuugug     1020

aaggccagcc uccccgagaa auaccaagaa aucuucgcag acucuuccaa ggauggauau     1080

gcugguuaca uagagggcaa gacuaaccag gaggccuuuu acaaauaucu gucaaaacuu     1140

cugaccaaac aggaagggag cgaguacuug cucgaaaaga uuaaaaauga ggacuuccug     1200

aggaagcaac ggacauuuga uaacggaagu aucccucauc agguacacuu aaccgaacug     1260

cgagcuauca uucgcagaca gucugaguau uacccauucc uaaaggagaa ccucgacagg     1320

aucgaaaaga uucugacguu ucggauaccc uauuacguug gcccucuugc ccgcgagaag     1380

uccgauuucg cauggaugac uagaaaaaca gacgauagca ucaggccaug gaauuuugaa     1440

gacuuggugg auaaggagaa aucggccgag gcuuucauuc aucguaugac caacaaugac     1500

cuguaucucc cggaagagaa gguccugccc aaacacucuc uuaucuacga aaaguucacu     1560

guguacaacg agcugacaaa ggugcgguuu uuggcggaag guuucaaaga cuuucaguuc     1620

cucaaucgaa agcaaaaaga gaccaucuuu aacucccugu ucaaggagaa aagaaagguc     1680

acggaaaagg auauuauauc auuuuuaaau aaaguugacg gguaugaggg aaucgcaauu     1740

aagggcaucg aaaaacaguu caacgccagu cugagcaccu accacgaucu aaagaaaauu     1800

cucggcaagg acuuucugga uaauacagac aacgagcuua ucuuggaaga uauagugcag     1860

acucugaccc ucuucgagga cagggagaug auccgcaagc ggcuggauau uuauaaagac     1920

uuuuucacag aaucccaguu aaagaaacug uacagaaggc auuauacugg guggggacgc     1980

cuuucugcua aguugaucaa ugguauucga aacaaagaga aucaaaagac cauccucgau     2040

uaccugauag acgauggcag cgccaaccgg aauuuuaugc agcuaauuaa ggacgcaggg     2100

cugucauuca aaccuaucau ugacaaggcc cguacaggaa gucacuccga uaaccucaaa     2160

gaaguagugg gcgagcuggc ugguagccca gccaucaaga aagggauacu ucagucuuug     2220

aagaucgucg acgaacuggu gaaaguuaug ggcuaugagc ccgagcagau uguggucgaa     2280

auggcaagag agaaccaaac cacugcgaag ggauuaucaa ggagucgcca gcggcucacg     2340

acacugagag aaagccuugc uaaucugaag uccaacaucu uggaggagaa aaagccuaaa     2400

uacgugaagg aucagguaga aaaucaucac cucucugacg auaggcuguu ccuauacuau     2460

cugcagaacg gcaaagacau guacaccgau gacgagcucg auauugacaa ucugucgcaa     2520

uaugauaucg accauauaau uccacaggcc uuuaucaagg acgauagcau cgacaaccga     2580

guucuugugu ccucagcaaa gaaucgcggg aaaagugaug acguccccuc uguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa uugcuggacg ccaaguuaau gagccagcgg     2700

aaguacgaua accucacuaa agcugagaga ggaggucuga ccuccgacga uaaggccagg     2760

uuuauccaac gucagcuugu ugaaacacgc cagauuacca aacacguggc acggauacug     2820

gacgagagau ucaauaacga gcuagauuca aagggcaggc gaauccggaa aguaaagauu     2880

gucacucuca agagcaaucu ggugucuaac uuucgcaaag aguucggguu uuauaagauc     2940

agagaaguua auaacuacca ccaugcucac gacgccuauu ugaaugcggu ggucgccaaa     3000

gcuauucuga caaaguaccc gcaguuagaa ccugaguucg uguacggaga uuaucccaaa     3060

uacaacuccu auaagacgag gaagagugca accgaaaaac ucuuuuucua cagcaacauc     3120

augaauuucu ucaagacuaa aguaacacug gccgacggca ccgugguugu caaggacgau     3180

auagagguga acaaugacac aggugagauc gugugggaua aaaagaaaca uuucgcuacu     3240

guccggaagg uucuuucuua uccacaagug aacauuguga agaaaaccga aauccagacc     3300

gggggcuuuu ccaaggaguc aauucuggcc cacggaaaua gugacaaauu gaucccucga     3360

aagacgaaag auauauaccu cgaccccaag aaguauggcg gguucgauag cccaauugua     3420

gcauacucgg uccugguggu ugcggacauc aaaaagggaa aagcccagaa gcuaaaaaca     3480

gugacugaac ugcuugguau caccauuaug gagagauccc gcuuugaaaa gaaccccucu     3540

gcuuuccucg agagcaaggg cuaccugaau auaagggaug acaaauugau gauccugccu     3600

aaguauucau uauuugagcu cgaaaacggg cgucggagac ugcuugccag ugcaggagag     3660

cugcagaaag gcaaugaauu ggcucuccca acacaauuca ugaaguuucu guaccuagcc     3720

uccagguaua acgagcugaa agguaagccg gaggaaauug agcagaagca ggaauuuguc     3780

gugcagcacg uuucuuacuu ugaugacauc cuucaaauua ucaaugauuu cagcaaccgc     3840

gugauacucg cagacgccaa ucuggagaaa aucaacaagu uguaucagga caauaaagaa     3900

aacauucccg uagaugaguu agcuaacaau aucauuaacc uguuuacuuu caccucccuc     3960

ggggcgccug ccgcauuuaa guucuucgac aaaaucgucg aucgaaagag auacacguca     4020

acaaaagagg ugcugaauag cacccuuaua caucagagua uuacuggccu guaugaaaca     4080

cggaucgacu ugggaaagcu aggcgaggau uga                                  4113


<210>  3143
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3143
augaagguga acaauuacca caucggccug gacauuggga ccagcuccau aggauggguc       60

gccaucggug aggauggcaa accccucaga auuaagggga aaacagcuau cggagcaagg      120

cuuuuccagg aaggcaaccc ugccgcggac cggcgcaugu uucgaacuac gcguagaagg      180

cugucucggc gcaaguggag auugaaauua cuggaggaaa uuuucgaucc auauaucacc      240

ccgguugacu caacauuuuu cgcuaggcua aagcaaagua aucucagccc caaggauucc      300

cggaaagagu uuaaggguuc uaugcuguuc ccugaccuua cugauaugca guaccauaaa      360

gacuauccaa ccauauacca ucugcgccac gccuugauga cacaggauga aaaguuugac      420

auccgaaugg uguaucucgc aauucaccau aucguaaaau acagagggaa cuuccuaaau      480

ucgacccccg uggauucauu uaaggccagc aaggucaacu ucguggacca guuuaaaaag      540

cugaaugagc ucuauacugc uauuaacccu gaggaaaguu uccaaaucaa uuuagcaaac      600

uccgaggaua uaggacacca guuucuggac ccaucuauua ggaaauucga caagaaaaag      660

cagaucccca aaauuguucc ugugagcguc gaugacaagg ccacagauaa gaucaauggc      720

aaaaucgcuu ccgaaauuau aaacgccauc cuuggcuaca agucaaaauu ggacguggua      780

guucagugca ccccagugga uaguaagagc ugggcgcuga aauucgacga ggaggauauu      840

gacgcaaagc uacaaaagau ccucccggaa auggaugaga aucagcaguc uauaauugcc      900

auccugcaga accuuuacuc ccaagucacg cugaaucaga ucgugcccaa cgggauguca      960

uugagcgaaa guaugauuga gaaauauaac gaccaccaug aucaccucaa gcuguacaaa     1020

aagaucauug accaguuagc ugacccuaaa aagaaggccg cacugaaaaa ggcuuauucu     1080

caauacgugg gagaugacgg uaaagucaua gaacaggccg auuuuugguc cagcguuaag     1140

aaaaaucucg acgauucgga ccuuucuaag cagaucaugg aucugauuga cgcggagaag     1200

uucaugccaa aacagcggac uucccaaaac ggcgugaucc cccaucaguu gcaccagcgu     1260

gagcuagaug aaaucauuga gcaccaguca aaguauuacc cuuggcuggc cgaaauaaau     1320

ccaaacaaac augaccucca ccuggcaaag uauaaaaucg aggaacuugu agcuuuucgc     1380

gugcccuacu acgucgggcc gaugauuacc ccugaugacc aagccaagag ugcugagaca     1440

guguucagcu ggauggagag aaaaggaaag gaagccggcc agaucacucc cuggaauuuu     1500

gaugagaagg uugacaggaa cgcauccgcc aaucgguuca uuaaacgaau gaccacaaag     1560

gacaccuauc ugaucgguga agauguguug ccagacgagu cucuccugua cgaaaaauuu     1620

aaggucuuaa acgagcugaa uauggugcgc guaaacggga aacuacucaa gguugcugau     1680

aagcaggcga uauuccagga ccuguuugag aauuauaaac auaucagcgu gaagaaacuu     1740

caaaacuaca uuaagucaaa aacgggauug ccuagugauc ccgaaaucuc cggccugagc     1800

gacccagagu auuucaauaa cucucucggc acuuacaaug auuuuaagaa gcuguucggg     1860

aacaaagucg acgaacccga ucuucaggac gauuuugaga agauugugga guggucaaca     1920

gucuucgaag acaaaagaau ccugagggag aaguugaacg aaauaaccug gcucagugau     1980

cagcagaaag acgugcugga gagcucccgg uaucaaggau ggggucgcuu aucuaagaag     2040

cugcuaacug gcauuguuaa ugaccagggg gaaagaauca uugaugagcu cuggaacaca     2100

aauaaaaacu ucaugcagau ccaaucggac aaugauuuug caaagaggau acacgaggcc     2160

aacgcugacc agaugaaagc cguggaugua gaagacgucc uggcagaugc cuacaccagc     2220

ccucagaaua agaaagcuau ccgucaggug guuaaggugg ucgacgauau ucaaaaagcc     2280

augggaggcg uggcaccaaa guacaucucc auugaguuca cgcggucaga agaccgaaac     2340

ccgagaagga ccaucagucg ccagcggcag cuugagaaua cauugaagga uacugcgaaa     2400

ucucuggcua agagcauaaa ccccgaacuc cuguccgagu uagacaaugc cgcaaaauca     2460

aagaaagguc ugaccgacag acuuuauuug uacuuuacac agcucgggaa ggauauuuau     2520

acuggcaagc cuaucaacau cgacgagauu agcaccuacg auaucgacca uauucugcca     2580

caagccuucg uaaaagauga cucucuaaau aacagggugc ugguuuccaa ggcuauaaau     2640

aacggaaaaa gugauaacgu ccccgugcag cucuuuggcg ccaagauggg gcacuucugg     2700

aaacagcugg cagaagcugg acuuaucagc aagcgcaagu ugaaaaaucu gcagacagac     2760

ccugauacca uuucuaagua ugccaugcac gguuuuaucc gacggcaauu aguggagacu     2820

ucccagguca uuaaacucgu ugcgaacauc cugggcgaca aguaccguaa ugauaacacg     2880

aaaauaaucg aaauuacagc cagaaugaau caucagauga gggacgaguu cggguuuauc     2940

aagaaccgcg aaauuaauga uuaucaccau gcuuucgacg cauaccuuac cgccuuucug     3000

ggacgguacu uguaucacag auacaucaag cucaggccau auuucgugua cggcgacuuu     3060

aaaaaguuca aagaggauaa ggugacuaug cgaaacuuca auuuucugca cgaccuaacc     3120

gaugacacac aagagaaaau agcugaugcc gaaaccggug agguaauuug ggaccgcgaa     3180

aacucaaucc agcagcugaa ggaugucuau cauuacaagu ucaugcucau cagucacgag     3240

guguauacuc ugagaggggc aauguuuaau cagacaguuu accccgcgag cgacgccggc     3300

aaacggaaac uuauuccgau aaaagcugau aggccuauca acguguacgg aggcuauucg     3360

ggguccgccg acgcauacau ggcuauuguc cguauccaua auaagaaagg agauaaguau     3420

cgcgugguug gugugccaau gcgggccaga gacagguugg augcagccaa aaagguaucu     3480

gacgcugacu gugaucgagc gcugaaggac gucuuaacgc cccaacucac caaaacuaag     3540

aaaagccgga agacaggcga gauuacccag gugguggaag auuucgagau cguccugggg     3600

aaaguuaugu accgccagcu uaugauagac ggagauaaga aguuuaugcu gggcucaagu     3660

acauaucagu acaacgccaa acaacuggug cucuccgacc agucuguaaa gacucuggca     3720

agcaaaggua gauuggaucc ucugcaggaa uccauggacu auaauaacgu guacaccgag     3780

aucuuagaua aggucaacca guacuucuca cucuaugaca ugaauaaauu uaggcacaag     3840

cugaaccuug gguucagcaa guuuauuagu uuccccaauc auaacguguu ugauggcaau     3900

acgaaggcuu cuuccggaaa gcgggaaauc cuggaggagg uuuugaacgg ccuccacgcc     3960

aauccaaccu ucgggaaccu gaaagacauu ggaaucacaa cuccuuucgg ccaacuacag     4020

cagcccaaug guauacugcu uagcgacgaa gccaagauuc gauaccaguc gccaaccggg     4080

cucuuugaga gaacaguguc acugaaagau uuguga                               4116


<210>  3144
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3144
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggauccgc      240

uaucugcagg agauuuucag cuccgaaaug ucuaaaguag acgauucguu uuuccauaga      300

cuagaggaau cauuucucgu ggaggaggac aagaaacacg aaaggcaucc uaucuucgga      360

aacauagucg augagguggc uuaccacgaa aaguauccaa ccaucuacca ucugcggaag      420

aaacuugccg acagcacaga uaaggcagac cugcgcuuga uuuaucucgc ccuggcucac      480

augaucaaau uucgaggcca cuuccugauu gagggugauc ucaauccgga caacagugac      540

auggauaagu uauuuaucca acugguucag accuacaauc agcuuuucga ggaaaacccc      600

auaaaugcau ccagagugga cgccaaagcu auuuugucug ccaggcugag caagucccgg      660

cgucuagaga accucaucgc gcagcugccu ggggaaaaac gcaauggacu uuuuggcaac      720

cugauugcau ugucacucgg ccugacucca aauuucaaga guaacuuuga uuuagccgag      780

gacgcuaagc ugcaacucag caaagauaca uacgacgaug accuugauaa ucuguuggcc      840

cagaucgggg accaguaugc agaucuauuc cuggcugcca agaaccucuc ugacgcgauc      900

cugcuuuccg acauucugag agucaacuca gaaauaacca aagcccccuu gagcgcaagu      960

augaucaaga gguacgauga gcaucaccag gaccucacgc uguuaaaagc ucuggugcgg     1020

caacagcuac cugagaagua uaaggaaauu uucuuugauc agucuaaaaa uggauacgcc     1080

gguuauaucg acggcggggc uucccaggag gaguucuaca aguuuauaaa accaauucuc     1140

gagaagaugg auggaacuga agaacugcuu gccaaauuga accgagagga ccugcuccgc     1200

aagcaaagaa ccuucgauaa uggcagcauc ccccaucaga uccaccuggg ugaacuucac     1260

gcaauucuga ggcggcagga ggacuuuuau ccuuucuuga aggauaaccg cgaaaagauc     1320

gagaagauuc ucacauuuag aauaccauac uacguagggc cgcuggccag gggaaauucg     1380

cguuucgcuu ggaugacucg gaaaucugaa gagaccauca cacccuggaa cuuugaggaa     1440

guuguggaca agggcgcguc cgcacaauca uucauugagc gaaugaccaa uuuugauaaa     1500

aacuuaccua augaaaaggu ccugccaaag cauagucuac ucuaugagua cuucacggug     1560

uauaacgagc ugacuaaagu gaaguacguc acagaaggca ugagaaaacc cgaguuucuu     1620

agcgggaagc agaaagaagc caucguugac uugcuguuca agaccaauag gaaagugacu     1680

guaaagcagc ucaaggagga uuauuucaaa aagaucgaau gcuuugacuc cguggagauu     1740

ucuggagucg aggaccgcuu caacgcuagc cuggguacau accacgauuu acugaaaaua     1800

aucaaggaca aagauuuucu ugacaaugaa gagaacgaag auauuuugga ggacaucgug     1860

cucacccuga cgcuauucga agauaaggag augauugagg aacggcugaa gaaauaugcc     1920

aaucucuuug acgauaaggu uaugaaacag cugaagagaa ggcauuacac cggcuggggg     1980

cgccuuucac gaaaauugau caacggaaua cgggacaagc aaaguggcaa gacaauccug     2040

gauuucuuaa aauccgacgg uuuugcaaac cguaauuuca ugcagcucau uaacgaugac     2100

agccugacuu uuaaggaggc cauccagaaa gcucaggugu cugggcaagg ccacucacuu     2160

caugaacaga uugccaaucu ggcaggaagu ccugcgauca agaaaggcau auugcagagc     2220

gucaaggugg uagacgagcu cguuaaggug auggggcaca aaccagaaaa cauugucauc     2280

gagauggcua gagagaauca gaccacacaa aagggacaga aaaacuccag ggaacgcaug     2340

aagcggauug aggaagguau caaagagcug ggcucucaga uacuaaagga gcaccccgug     2400

gaaaauacuc agcugcaaaa cgagaaacuc uaccuguauu accuucagaa ugggagagau     2460

auguaugucg accaggaauu ggauaucaac aggcugucgg acuacgaugu ggaccauauu     2520

guuccgcaga gcuucaucaa ggaugacucc auugauaaua agguguuaac ccgaucagac     2580

aaaaaccgcg gaaagaguga uaauguaccu ucugaggaag ucgugaaaaa gaugaaaaac     2640

uauuggcggc aacuccugaa ugccaaacuu aucacacaga gaaaguuuga caaccugacc     2700

aaagcagaga ggggcggucu aagcgagcuc gacaaggccg gguucauaaa acgucagcug     2760

guugaaacuc gccaaauuac gaagcacgug gcucagaucu uggauucccg gaugaacaca     2820

aaauacgacg agaaugauaa gcugaucaga gaagucaagg ugauuaccuu aaaaucaaag     2880

cucguaagcg acuuuaggaa agauuuccag uucuauaagg ugcgagagau caacaauuac     2940

caucacgccc acgacgcaua ccugaacgcu guugucggca cugcccuuau uaaaaaguau     3000

cccaagcugg aaucugaguu uguguacgga gauuauaaag uguacgacgu ccggaagaug     3060

auagcgaaau ccgagcagga aaucggcaag gccaccgcua aacgcuucuu uuauaguaau     3120

auuaugaacu ucuuuaagac agagaucacc uuggcaaaug gggaaauuag aaagaggcca     3180

cucaucgaga cuaacgaaga gacaggagag auaguuuggg auaaaggucg ggacuucgcc     3240

acggugcgaa aggugcugag caugccucaa guaaauaucg ucaaaaagac cgaagugcag     3300

acuggcgcuc uaacaaacga gucuauuuac gccagagggu ccuuugauaa acugaucuca     3360

cgcaagcaua gguucgaaag uagcaaauau ggaggcuuug guucgcccac cguuacauac     3420

uccgugcuug ucguggcaaa gucuaagguu caggacggga aagugaagaa aauuaagacu     3480

ggcaaagagc ucaucggaau aacccuguug gauaagcugg uauucgagaa gaauccauua     3540

aaauuuauug aagacaaggg cuacgggaac guccagaucg acaaauguau caagcucccc     3600

aaauauagcc uguucgaguu cgaaaaugga acgcgucgga ugcuugcguc agugauggcc     3660

aacaauaaca guagaggcga ucugcaaaag gcuaacgaga uguuuuugcc ugccaagcuc     3720

gugacccugc uauaccacgc acauaaaauu gaauccucua aggagcugga gcacgaggcu     3780

uauauacuug accauuacaa ugaucucuau cagcuguuga gcuacaucga aagguucgcc     3840

uccuuauaug ucgacguuga gaaaaacauu ucaaagguga aagaacuguu uagcaauauc     3900

gaaaguuacu cuauuuccga gaucugcagc ucgguaauaa accuccugac acuuacugca     3960

ucaggugccc cagcugauuu caaguuucug gggaccacaa ucccgcgcaa gcgauacgga     4020

ucuccccaga guauuuuguc cagcacucua auccaccaau cuauuaccgg ccuguaugag     4080

acgagaaucg accucagcca gcuggguggc gauuga                               4116


<210>  3145
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3145
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggauccgc      240

uaucugcagg agauuuucag cuccgaaaug ucuaaaguag acgauucguu uuuccauaga      300

cuagaggaau cauuucucgu ggaggaggac aagaaacacg aaaggcaucc uaucuucgga      360

aacauagucg augagguggc uuaccacgaa aaguauccaa ccaucuacca ucugcggaag      420

aaacuugccg acagcacaga uaaggcagac cugcgcuuga uuuaucucgc ccuggcucac      480

augaucaaau uucgaggcca cuuccugauu gagggugauc ucaauccgga caacagugac      540

auggauaagu uauuuaucca acugguucag accuacaauc agcuuuucga ggaaaacccc      600

auaaaugcau ccagagugga cgccaaagcu auuuugucug ccaggcugag caagucccgg      660

cgucuagaga accucaucgc gcagcugccu ggggaaaaac gcaauggacu uuuuggcaac      720

cugauugcau ugucacucgg ccugacucca aauuucaaga guaacuuuga uuuagccgag      780

gacgcuaagc ugcaacucag caaagauaca uacgacgaug accuugauaa ucuguuggcc      840

cagaucgggg accaguaugc agaucuauuc cuggcugcca agaaccucuc ugacgcgauc      900

cugcuuuccg acauucugag agucaacuca gaaauaacca aagcccccuu gagcgcaagu      960

augaucaaga gguacgauga gcaucaccag gaccucacgc uguuaaaagc ucuggugcgg     1020

caacagcuac cugagaagua uaaggaaauu uucuuugauc agucuaaaaa uggauacgcc     1080

gguuauaucg acggcggggc uucccaggag gaguucuaca aguuuauaaa accaauucuc     1140

gagaagaugg auggaacuga agaacugcuu gccaaauuga accgagagga ccugcuccgc     1200

aagcaaagaa ccuucgauaa uggcagcauc ccccaucaga uccaccuggg ugaacuucac     1260

gcaauucuga ggcggcagga ggacuuuuau ccuuucuuga aggauaaccg cgagaaaauc     1320

gaaaagauuc ucacauuuag aauaccauac uacguagggc cgcuggccag gggaaauucg     1380

cguuucgcuu ggaugacucg gaaaucugaa gagaccauca cacccuggaa cuuugaggaa     1440

guuguggaca agggcgcguc cgcacaauca uucauugagc gaaugaccaa uuuugauaaa     1500

aacuuaccua augaaaaggu ccugccaaag cauagucuac ucuaugagua cuucacggug     1560

uauaacgagc ugacuaaagu gaaguacguc acagaaggca ugagaaaacc cgaguuucuu     1620

agcgggaagc agaaagaagc caucguugac uugcuguuca agaccaauag gaaagugacu     1680

guaaagcagc ucaaggagga uuauuucaaa aagaucgaau gcuuugacuc cguggagauu     1740

ucuggagucg aggaccgcuu caacgcuagc cuggguacau accacgauuu acugaaaaua     1800

aucaaggaca aagauuuucu ugacaaugaa gagaacgaag auauuuugga ggacaucgug     1860

cucacccuga cgcuauucga agauaaggag augauugagg aacggcugaa gaccuaugcc     1920

caucucuuug acgauaaagu uaugaagcag cugaaaagaa ggcacuacac aggcuggggg     1980

cgccuuucac gaaaguugau caauggaaua cgggacaaac aaaguggcaa gacuauccug     2040

gauuucuuaa aguccgacgg uuuugcaaac cguaacuuca uucagcucau ccaugaugac     2100

agccugaccu uuaaagaggc cauucagaag gcucaggugu cugggcaagg ccacucacuu     2160

cacgaacaga ucgccaaucu ggcaggaagu ccugcgauua aaaagggcau auugcagagc     2220

gucaaagugg uagacgagcu cguuaaggug auggggcaua agccagaaaa caucgucauu     2280

gagauggcua gagagaauca gacaacucaa aaaggacaga agaacuccag ggaacgcaug     2340

aaacggaucg aggaagguau aaaggagcug ggcucucaga uccuaaaaga gcaccccgug     2400

gaaaauaccc agcugcaaaa cgagaagcuc uaccuguauu accuucagaa ugggagagau     2460

auguaugucg accaggaauu ggauauuaac aggcugucgg acuacgaugu ggaccauauc     2520

guuccgcaga gcuucauuaa agaugacucc aucgauaaua agguguuaac acgaucagac     2580

aagaaccgcg gaaaaaguga ugacguaccu ucugaggaag ucgugaagaa aaugaagaau     2640

uauuggcggc aacuccugaa cgccaaacuu auaacccaga gaaaguuuga caaucugacu     2700

aaggcagaga ggggcggucu aagcgagcuc gauaaagccg gguucauuaa gcgucagcug     2760

guugaaacgc gccaaaucac aaaacacgug gcucagaucu uggacucccg gaugaacacc     2820

aaguacgaug agaaugacaa acugauuaga gaagucaagg ugaucacuuu aaagucaaaa     2880

cucguaagcg auuuuaggaa ggacuuccag uucuauaaag ugcgagagau uaacaacuac     2940

caccaugccc acgaugcaua ccugaaugcu guugucggca ccgcccuuau aaagaaauau     3000

cccaagcugg aaucugaguu uguguacgga gacuauaagg uguacgaugu ccggaaaaug     3060

aucgcgaagu ccgagcagga aauuggcaaa gccacagcua agcgcuucuu uuauaguaac     3120

aucaugaauu ucuuuaaaac cgagauuacu uuggcaaacg gggaaaucag aaagaggcca     3180

cucauagaga caaaugaaga gacgggagag aucguuugga acaagggucg ggacuucgcc     3240

accgugcgaa aagugcugag caugccucaa guaaauauug ucaagaaaac ugaagugcag     3300

acaggcgcuc uaaccaacga gucuaucuac gccagagggu ccuuugauaa gcugauuuca     3360

cgcaaacaua gguucgaaag uagcaaguau ggaggcuuug guucgcccac aguuacuuac     3420

uccgugcuug ucguggcaaa aucuaagguu caggacggga aggugaaaaa gaucaaaacc     3480

ggcaaggagc ucauaggaau uacgcuguug gauaaacugg uauucgagaa gaauccauua     3540

aaguuuaucg aagacaaagg cuacgggaac guccagaucg acaaguguau uaaacucccc     3600

aaguauagcc uguucgaguu cgaaaaugga acccgucgga ugcuugcguc agugauggcc     3660

aacaauaaca guagaggcga ucugcaaaaa gcuaaugaga uguuuuugcc ugccaagcuc     3720

gugacacugc uauaccacgc acauaagaua gaauccucua aagagcugga gcacgaggcu     3780

uauauccuug accacuacaa cgaucucuau cagcuguuga gcuacauuga aagguucgcc     3840

uccuuauaug ucgacguuga aaagaacauc ucaaaaguga aggagcuguu uagcaauauu     3900

gaaaguuacu cuaucuccga gauaugcagc ucgguaauca accuccugac ucuuaccgca     3960

ucaggugccc cagcugauuu caaauuucug gggacaacua uuccgcgcaa gcgauacgga     4020

ucuccccaga guaucuuguc cagcacccua auucaucaau cuaucacggg ccuguaugag     4080

acaagaauag accucagcca gcuggguggc gauuga                               4116


<210>  3146
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3146
augaacaaua acaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucauaacag augacuauaa gguucccucu aaaaagauga gagugcucgg uaauacugau      120

aaaagguuca ucaagaaaaa ccuuauuggc gcucuguugu uugacgaggg gacgaccgca      180

gaagaucggc gcuuaaagcg aacagcccgu agaagguaca cucggcgcaa gaauagacug      240

agguaucuac aggagaucuu cgcggaagag augucaaaag uagacaguag cuuuuuccac      300

cggcucgaug acuccuuucu ggugccugaa gauaagucug gaucgaaaua cccaauuuuc      360

gcuacccuug ccgaggagaa ggaauaucau aaaaaguuuc cgacaaucua ccaucugcgc      420

aagcacuugg cagacucaaa agagaaggcc gaucuccgac ugauauaucu ggcucucgca      480

cacaucacca aauacagagg ccauuucuua uacgaagagg ccuuugacau uaagaacaau      540

gauauccaga aaauuuucaa cgaguuuauc aauauauaug acaacacuuu cgaagguagc      600

agucuguccg ggcaaaaugc ucaggucgag gccauuuuua caggaaagau cucuaaaagc      660

gugaagaggg aacacguucu uaaguuguuc cccgacgaga aauccaccgg ccuguucuca      720

gaauuucuaa agcucauugu gggcaaccag gcggauuuca aaaagcacuu ugaccuggag      780

gagaaagcaa gucuucaauu cagcaaggau acguacgacg aagaucugga gaauuugcuc      840

gggcagaucg gagacgauuu ugccgaccug uucuuagucg cuaagaaacu guaugaugcc      900

auccuccuuu cugguauucu gacugugacc gacccuucca caaaggcacc auugucagcu      960

agcaugauag aacgguacga gaaccaucag gaagaucuag ccgcgcugaa acaguuuauc     1020

aagaacaauc uccccgagaa auaugccgag guauucagug accaaucuaa ggacggcuac     1080

gcaggguaua uugauggaaa gacuacccag gaagcguuuu acaaauacau caagaaccug     1140

cuuuccaaau ucgagggcgc cgacuauuuu cuggauaaga uagaacguga ggacuucuug     1200

cgcaaacaga gaacauuuga uaaugguagc auuccucacc agauccaucu ccaagaaaug     1260

aacgcuaucc ugaggcggca gggggagcac uacccauuac ugaaggagaa uaaggagaaa     1320

auugaaaaga uccuaaccuu caggauuccc uauuacguug gaccucucgc ccgcggcaac     1380

agagacuucg cauggcugac gcgaaauucg gaucaggcca uacggccaug gaacuuugaa     1440

gagaucgugg acaaggcuuc uuccgcggag ucauucauua auaaaaugac uaacuaugau     1500

cuuuacuugc cggaagagaa aguccugccc aagcacaguc uccuguauga aacauuugca     1560

guguacaaug agcuuaccaa agugaaguuc aucgccgaag gccugcgcga cuaccaauuu     1620

uucgauagcg ggcagaagaa acagaucguc aaccaguugu uuaaggagaa aagaaagguu     1680

acugagaaag acauuauaca uuaucuccac aauguggaug gauacgacgg uaucgaacug     1740

aagggcauug agaagcaauu caacgcuucc uuaucuacau aucaugaccu gcuaaaaauc     1800

auuaaggaua aagaauuuau ggacaaucac aagaaccagg agauccucga aaacauagua     1860

cauacccuga cgaucuucga ggauagggag augauuaaac agcgucuugc ccaguacgac     1920

agcaucuuug augaaaaggu gauuaaggca uugacccggc gacacuauac agggugggga     1980

aaacugucag ccaagcucau caauggcaua ugcgacaaaa agacugguaa aaccauucug     2040

gauuacuuaa ucgacgaugg guauaacaau agaaacuuca ugcaacugau uaaugacgau     2100

ggccuuaguu uuaaggagau cauacagaag gcucaggucg ugggaaaaac agacgauuug     2160

acucagguug ugagggaacu cuccggcagc ccugccauca agaaagggau ucugcaaucu     2220

aucaagauug ucgacgagcu agugaaaauc augggauacg caccagaauc aauaguaauu     2280

gagauggcgc gcgagaacca gaccacagcu aaggguaaaa agaauaguca gcagcgguac     2340

aagagaaucg aagacgcccu gaaaaaccuc gcacccggcc uggauagcac caucuccaag     2400

gagaauccua cugacaacau ucaacuucag aaugauaggu uguuccugua uuacuuacag     2460

aacgggaaag acauguauac gggagaagcc cucgauauca aucaacuguc uucguacgac     2520

auugaucaca uaaucccaca ggcuuucauu aaggacgaua gccuugacaa ccgcguucug     2580

acauccucaa aagauaaucg aggcaagagu gacaacgugc ccucuuugga ggucgugaag     2640

aaacggaagg ccuuuuggca gcagcuccug gacagcaaac uaaucuccga gcguaaguuc     2700

aacaaucuga ccaaagcaga aagagguggg cucgaugaga gggacaaggu cggcuuuauu     2760

aagcgccaac ugguggaaac ucggcagauc accaaacaug uugcuagaau acuugaugcc     2820

agguucaaca cagagguguc agaaaagaau cagaaaaucc gaagcguaaa gauuaucacc     2880

uugaaaucua accuggucuc caauuuucgc aaggaguuca aguuauauaa agugcgggag     2940

auuaacgacu accaccaugc gcacgaugcc uaucucaaug cuguuguggc aaaggccauc     3000

cugaaaaagu acccgaaacu ugaaccugag uuugucuacg gagacuauca gaaguacgau     3060

cugaagagau auauaaguaa aagcaaggac cccaaagaag uggagaaggc uacugaaaaa     3120

uacuucuuuu auucuaaccu acucaauuuc uuuaaggagg agguacacua cgccgauggc     3180

acaauuguga aaagggaaaa caucgaguau uccaaggaca cgggggaaau cgcauggaau     3240

aaggagaaag auuucguuac cauuaagaaa guccugucau acccacaagu gaacauagug     3300

aagaaacgug aaguccagac uggagguuuc aguaaggaga gcaucuugcc uaagggcaau     3360

ucggacaaac ugauuccccg caagacaaaa gauaucuuau gggacaccac aaaguacggg     3420

ggauuugacu ccccaguuau ugcguauucu auccuccuga uagccgauau cgagaaaggc     3480

aaggcuaaga aacuuaagac ugugaaaacc cuggugggua uuacgaucau ggaaaaggcc     3540

gcauucgaga aaaaccccau uaccuuuuug gaaaauaagg gguaccauaa cguacggaag     3600

gagaacaucc ucugucugcc uaaauauagc cuauucgagc uggaaaaugg cagaaggcga     3660

cuucucgcuu cagccaagga gcugcagaaa ggaaacgaaa uagucuugcc aguguaccug     3720

acaacuuuac ucuaucacag uaagaauguu cauaaacugg acgagccgga acaccuugag     3780

uacauucaga agcaucggua ugaguuuaaa gaucuguuga accucguguc cgaguucucu     3840

caaaaguacg uccuggcaga agccaaucua gaaaagauca aaagccugua cguggacaac     3900

gagcaggcug auaucgaaau ucuugcuaau uccuuuauca accuccugac cuucacagcc     3960

uugggcgcac ccgcugccuu uaaguucuuu gggaaagacg uugaucgcaa gagauauacu     4020

accgugucag agauauuaaa ugccacgcug auucaccaga gcaucacagg acucuacgag     4080

accaggauug accugaguaa acuuggcgaa gauuga                               4116


<210>  3147
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3147
augaccgaga agaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucauaacug augacuauaa aguucccgcu aagaaaauga aggugcucgg uaacacggau      120

aaaaaguaca ucaagaaaaa ucuucugggc gcauuguuau ucgacaacgg ggaaaccgcc      180

gaggcgacaa gacugaagag gacugcucgg cgccgauaua cccguagaaa gaauaggcua      240

cgguaccucc aggaaauuuu ugccgaggaa auggcaaagg uagaugaguc uuucuuuuau      300

cgccuggacg agucauuccu uacaaccgau gacaaagauu uugaaagaca cccuaucuuc      360

ggaaacaagg ccgacgagau uaaguaccau caagaauuuc caacuaucua ucaccugagg      420

aaacauuugg cugauaguca cgagaaggca gaccuccggc ugauauaccu ggcccucgcu      480

cauaugauca aauuccgcgg ccacuuuuua auugagggug aacugaaugc cgagaacaca      540

gaugugcaga aacuuuucga agcguuuguc gagguguacg accgaaccuu cgacgauagc      600

aauuuguccg aaaucacggu ugacgcaucu ucgauucuga cugagaaauu uucaaagagc      660

agaaggcuag agaaccucau caaacacuau ccgaccgaaa agaagaauac acuguucggg      720

aaccuugugg cccuggcuuu gggacuccag cccaauuuca aaaccaguuu uaagcugucc      780

gaggaugcca aauuacaguu cucuaaggac acuuacgaag aggaucugga agaacucaua      840

ggcaaaauug gcgacgagua ugcagaucuu uuuacaagcg cuaagaaccu guacgacgcc      900

aucuugcuau ccgggauucu gaccgucgcg gauaauacga cuaaggcccc ucucucagca      960

aguaugauca aacgguauaa cgaacaucaa guggaccuga agaaacuuaa ggaguucauc     1020

aaaaacaaug cuagcgauaa guacgacgaa auuuuuaacg acaaggauaa aaauggauau     1080

gccgguuaca uagagaacgg cguaaagcag gacgaguucu acaaauaucu gaagacaacc     1140

uugucuaaaa ucgauggguc cgacuacuuu cucgauaaga uugagcguga ggacuuccug     1200

cgcaagcaga gaacuuuuga uaauggauca aucccacacc agauacauuu acaagaaaug     1260

cacgcuauuc ugaggcggca gggcgagcac uaugccuucc uaaaagaaaa ccaggcaaag     1320

aucgagaaaa uccucacauu ucggauuccc uacuauguug guccucuggc ccgcaagaau     1380

agcagauucg cuugggcgga guaccauagu gacgagaaaa ucaccccaug gaacuuugau     1440

gaaauuauag acaaggaaaa aucugcagag aaguucauca cgaggaugac ccuuaaugau     1500

uuguaucugc ccgaagagaa ggugcucccu aaacacuccc uggucuacga gacauacacu     1560

guguauaacg aacuuaccaa ggugaaauac gucaaugagc agggaaagag caacuucuuu     1620

gacgccaaua ugaaacaaga aauuuucgag cauguuuuua aggaaaaccg gaaggugaca     1680

aaagauaagu uccugaauua uuugaacaaa gaguuuccag aguaccgcau ccaggaccuc     1740

aucgggcugg acaaggaaaa caaaucguuc aaugcuucuu uaggcacuua ucacgaucug     1800

aagaagauuc uagacaaauc cuuucucgau gacaagacca acgagacaau aaucgaagau     1860

auuauccaga cccugacucu uuucgaggac agagauauga uuaggcaacg uuugcagaaa     1920

uacucagaca ucuuuacgcc gcagcagcug aagaaacucg aacggcgaca uuauacaggc     1980

ugggggagac ugaguuacaa guuaauaaau ggaaucagga acaaggagaa ugguaaaagc     2040

auucuggauu accuuaucga cgauggcuau gccaaccgca auuucaugca auugauuucc     2100

gacgauaccc uccccuuuaa gcagaucaua aaagacgcac agauuaucgg ggacauugau     2160

gacguaacuu cugugguccg ggagcugccu ggaagcccag ccaucaagaa aggcauacua     2220

cagucaguga agaucguuga ugaacuggug aaagucaugg gucacaaccc cgacaauauu     2280

gugaucgaga uggcuagaga aaaccaaacc acaaauaggg ggcgcaacca gagucagcag     2340

cgacucaaga agcugcaaga uucccuuaaa gaguugggca gcaauauucu gaacgaggaa     2400

aagccuucuu acaucgaggg aaaaguagaa aauaaccacu uacaggacga ucgacucuuc     2460

cuguauuaca uacagaacgg caaggacaug uauaccgggg augagcuuga cauugaucau     2520

cugucagacu acgacaucga ucacaucauu ccacaggccu uuaucaaaga cgauaguauu     2580

gacaaucgug uuuugacuag cuccgcaaag aacagaggaa agucugauga cgugcccucg     2640

cucgauauag uccaugacag gaaagcggau uggauccgcc uguauaagag cggucuaauu     2700

uccaaacgga aguucgacaa ucugacaaaa gcugaaagag gcgggcucac ggagaacgau     2760

aaggccggau ucaucaagag gcaacuggug gagacccgac agauuacuaa acacgucgca     2820

cagauccuug acucacgcuu uaauacagaa cgggaugaga acgacaaggu gauaagaaau     2880

guuaaaguga ucaccuugaa gaguaaccug guaucucaau ucaggaaaga cuuuaaguuc     2940

uacaaggucc gugaaauuaa ugauuaccac caugcccacg acgcuuauuu aaacgccgug     3000

guuggcacag cacuccugaa aaaguacccg aaacuugcuc cugaguuugu guauggugaa     3060

uacaagaaau augauauccg caaguucauu acuagcuccg gggacaaggc caccgcgaaa     3120

uacuuuuucu auucaaaucu gaugaacuuu uucaagcggg ucaucagaua cagcaauggc     3180

aaagugguag ugaggcccgu uauagagugc ucuaaggaua cgggagagau ugccuggaac     3240

aaacagaccg acuuugaaaa gguccgacgg gugcuauccu acccacaggu gaauaucguc     3300

aaaaagguug agacacagac uggcggguuc aguaaggaaa gcauccuccc uaaaggaaac     3360

ucugauaagc ugauuccccg caaaaccaag aaauucagau gggacacacc aaaguauggu     3420

ggcuuugauu cccccaacau agcuuacuca guguucguga ucgcagacgu agagaagggg     3480

aaagccaaga aauugaagac ugucaaagaa cuggugggaa uuaguaucau ggagaggagc     3540

ucguuugagg aaaauccugu uguguucuua gagaagaagg gcuaucaaaa cguccaggaa     3600

gauaaucuca uuaaacugcc aaaguacucc cuuuuugagu ucgagggugg gcggcgaaga     3660

cuguuggcuu cugccagcga acuccagaaa ggcaacgagg ugguucuguc acgccaucua     3720

guggaacugc uuuaucacgc acauagggua aauaguuuua acaauuccga gcaccucaag     3780

uacgucucug aacacaaaaa ggaguucgga gaggugcuga gcugugugga aaacuuugcg     3840

aaguccuaug ucgacguuga gaaaaauuug ggcaagaucc gugccguggc ugauaaaaua     3900

gacaccuucu caaucgaaga cauuagcauc aguuuuguaa accuguuaac gcucacagcc     3960

cugggggcac cggcugauuu caauuuucuu ggagagaaga uuccccggaa aagauacacc     4020

ucuacuaagg aaugccugaa cgccacauug auccaucagu ccauaaccgg ccucuacgag     4080

acuaggauug accugagcaa acuaggugag gaauga                               4116


<210>  3148
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3148
augaaccuga gaaaugugga gagcuaccag gucggccucg acaucgggac cggauccguu       60

gguugggccg ugcuugauga caacggcgau cugugcaggu ucaaggggaa acccacaugg      120

ggaucucggg uauuuccuac ugcugaaacg gcagccgagg cgcgcgugca ccgaggccaa      180

cguagaaggu augaccggcg cagacagagg auugauuugu uacagcgguu cuuugcugac      240

gaagucgcca agguggaucc agaguucuuu auacgccuga aucaggcacg acuacauccg      300

gaaaacagag accccgccca cgcugauuac agguggccuc ucuucaaugg uucaaacuuu      360

accgagaaag acuauuaccg gcaauuccca acaaucuauc aucugcgugc acaccuucug      420

aagagugaug agcccgccga cauucgcuug guuuaccucg cucugcauaa uaucgugaaa      480

cacagaggga acuuucugca ccaggauaau ccuagccucu ccgccaggaa cgcgaauguc      540

ggagacgcag uggaagccuu augucuggcu cuugacgagu ggugcggcgc ccaugaugua      600

gaaugugcau gcgacgaggc ugccuuggcg gaggcccugg gcgaugcauc ucuacggcca      660

ggggaaaagc gagagcgcgc ugaagccgcu cucggaguug agaaggccca caaaaagcug      720

gcaaaagccg ugucgucagc ugucgugggu uaugcggcag aauuuggcca uguguuuuuc      780

gccgagagcg aggggaguaa guuugcucuu uccaacgacg aaaaagucga ggccuacguu      840

ugucccgaug aaggaagaga ccuguucgau gcaaugaggg ccgugcacuc uagcuacgua      900

uugaugggca uucucuccga ggcucagggu gaacuguuau cagugugcaa ggucggggag      960

uaugagcggu acaagcgcga ccuggaaacu cucaaaaguc uugugagagc cuauggaccu     1020

gagagguacg augaguucuu uuguggccca uucuaugaag gcacccguga cuacgauccg     1080

gggaaggcac agggauauac aaaauacaau cugggugcga gcaaguuguc ucacgaagac     1140

uuucuaaaag agguucaaaa gcugcucaaa gacaucggcg cucaggccga ugagcgguac     1200

gcagacauag aacgagccau cgaggcuggg gccuuccuga gaaggcuuaa gaccuccgau     1260

aacggaacua uucccuauca gcugcauuug gaagagaugg acgcaaucau ugagcgccag     1320

gcugccuuuu acccuuuccu cgcgcaagaa aaggauaaac ugaagucauu agugagcuuu     1380

cggaucccau auuacgucgg cccccugaca cagaaaaaug ccgcuagaga cgguaagggg     1440

gaggcaaggu ucgccuggag uucucgcaaa ccuggcaugg aagaugcucg aguguaucca     1500

uggaacuggg aggacguaau agauaaggac caguccgccg aagauuuuau ucggcguaug     1560

accggaacgu gcacuuaccu aaagggcgag cccguucucg caagauguag ccuggacuau     1620

gagcuuuucu gcguguugaa cgaacuggcg ggggccaggu ggacccaaga uggagacaaa     1680

ucguaccgcu uugacgcugc cgaucggcuc gguaucgucg aggaccuguu caagaaaaga     1740

aagaggguga cauacgaagc agcugagaaa uggcuugccc gaacucacgg cccggaaucu     1800

gggaaguccg caggaggcgc cuaucauguc ucaggugggc agggcgagga ugcuuuugag     1860

agucagcuga gcucccacua cgacuucugu aagauuuugg gaguggauga acucucugac     1920

gcggaugagg ccauggcaga agacaucauc cuguggaaua ccguuuucga ggaucgcagc     1980

auuuuaaaac ggaagauaaa acuggcuuau ggcgacagac uaggggaugc ccaggugagg     2040

aagcucugcc aaaaacguua cacaggaugg ggucgccugu caggcgaacu uuugcggggg     2100

cugagaagug ccaccgacaa cggaccuaag acgaucaugg auauucuccu ggagggcgac     2160

cccaaucaug cuaggcgaau gggugcagcc augaacuuaa uggagauccu gcacgacgau     2220

cuuuuggaau uucaggcgaa gauagacgag cacaaucggg aacgcgcucu cgccguaggg     2280

ggcauguccg ucgaugaggu gacuggaagc ccagcacuga gaaggggcau uaaccaggcc     2340

cuacggguug uggacgagau cgcugggauc gcaggaaaac cucccgccca uauuuauguc     2400

gaagugacac gagaugaggg cgcgagaaau aagggucgca ggaccacuuc ucguuaccgg     2460

gcucuggaag ccgcacucuc aaguaucaaa gcccagugcg cugacguaua ugccgagcug     2520

aaggaaagag auccaagggc acuugacgau gagcgcuuga cacuguacuu caugcaaugu     2580

gggaaaugca uguacagcgg agacgcuuua gauauuaaca agcuccagac cuaucaggug     2640

gaccacauac ugccccaguc cuacguuaaa gacgauucuu uugagaauaa gguccuugug     2700

cgaccugaca cgaaccaaag aaagggcgau acccuguugc ucgacgaacg gcugaucgcc     2760

aggaugcgcg guuucuggcg uacacuaaga gaugcggagc ugauuggcga gaaaaaguuu     2820

aauaaccuca cucggaccuc ggugagcgac gaacagauga aaaaguucgu cuccaggcag     2880

cugguugaaa cagggcagau ggugaaacaa gugcagcagc uuuugcaagc ccgcuaucca     2940

gagucagagg uacagccggu caaggcagga gugagucauc agcugcgagu uggcugugcu     3000

uuagccaagu cuagagaaau caaugauuuu caccacgcuc augacgcaua ccucgccugc     3060

cagguggggc gguucgucca auauaggcac agcgccgugu acuccgagcc cguuaaaaug     3120

gcugcggugg uaaagcaguu uaucaaaaag caggcagaag auuaucgcag aacuagggag     3180

cugccuggau cagacggcuu cauugucagc ucuuuuauga ccccagguuu cgaugugaaa     3240

acaggggaag uguuucggga cgccugggau gcuggagccg agauagaccg uauccgccga     3300

uguuucgacu acaaggauug cuucauuucc agaaugcccg aggaaaccca uggcgcauuu     3360

ugggacgcca cuaucuacag uccuagggcu gcggguaaga cgcuuagccu gccacuaaaa     3420

cggggccucg auccccgcaa guaugggucu uacuccagag agcaguucgc cuauuucuuc     3480

gucuacgaag caguuaaacc gaagaaaaag caaagggugc uggaguuugc uccuguacca     3540

gugcgggucg ccucagcauu ggccaacgac cccgaugcuc uggaggacua ugcccgagaa     3600

uuagcggagg cagaaggacu cguguucgag cgcauuagaa ggcggaaggu uuacaaauau     3660

cagcagauca uagugggcga uagucgucug uacgucacag ggagagaaga ggugaggaau     3720

gcccgccagc uugcuuuuag ccaagacgag accaagcuga ucugucggau ugaacaggga     3780

gaggaaugcg aggaaggcga guugcucgau cuguuccgau cgaugcagag aaaauaccua     3840

gccugugcuc cuaggcuggc acgccuuaug aagguugccg agauggaaga ggguuuuauc     3900

gcugccacug cauccgggaa acgggaagug cuccugucuu ugaccagcau ugcggccgcu     3960

cacagaaacu caaucgaccu gaagcccaua ggacguaaag gcaguuccgg ucaaaugaag     4020

auuacauucu cuaaggaguu aagcaccaac ggcauccucu uugucgauuc cucaguaacu     4080

gggauguucg aaaggcggug ccgccuggga cuuuga                               4116


<210>  3149
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3149
auggagaaca agaauuacag caucggccug gacauuggga ccaacuccgu gggauggucu       60

gucauaacag augacuauaa aguucccuca aagaaaauga aggugcucgg uaauacugau      120

aaaagauuca ucaagaagaa ccuuauuggc gcccuguugu uugacgaagg gacgaccgcu      180

gaggcaaggc gguuaaaacg cacagcccga cguagauaca cuaggcggaa gaaucgccug      240

agauaucuac aggaaaucuu cgcggaggaa augaguaaag uagauagcuc cuuuuuccac      300

aggcucgacg auucuuuucu gauuccugag gacaagcggg gaucgaaaua cccaaucuuc      360

gcuacccuug ccgaggaaaa ggaguaucau aagcaauuuc cgacaauaua ccaucugcgc      420

aaacaguugg cagauucaaa ggagaaaacc gaccuccgac ugaucuaucu ggcccucgcu      480

cacaugauua aguacagagg ccacuucuua uacgaagaua cuuuugacau caaaaacaau      540

gauauucaga agaucuucag cgaguuuaua aguauuuaug acaacacauu cgaagguucc      600

ucucugagcg ggcagaaugc acaaguggag gccaucuuua ccgacaaaau uuccaaguca      660

gcuaagaggg aacggguccu uaaauuguuc cccgaugaga agaguacggg acuguucagc      720

gaauuucuaa aacucaucgu gggcaaccag gccgacuuca agaaacauuu ugaucugggc      780

gagaaggcgc cucuucaguu cucuaaggac acuuacgaug aggaccugga aaauuugcuc      840

gggcagaucg gagaugacuu ugcagaucug uucuuaguug ccaaaaagcu guaugacgcu      900

auucuccuuu ccgguauacu gaccgugaca gauccaucaa cuaaagcccc cuugagcgca      960

aguaugaucg agcguuacga aaaccaccaa aaggaccuag cuguccugaa acaguuuauu     1020

aagaauaacc ucccugagaa guaugacgaa guguucucug aucaguccaa agacggcuac     1080

gccggguaua ucgauggaaa gaccacacag gaggcguuuu acaaauacau aaagaaucug     1140

cuuagcaaau ucgagggcac cgacuauuuu cuggaaaaga uugagcgcga agauuucuug     1200

agaaagcaaa ggacguuuga caacgguucg aucccacacc agauccaucu ccaggagaug     1260

aacgccauuc ugcggcgaca aggggaacac uaccccuucu uaaaagauaa uaaggagaaa     1320

aucgagaaga uucugacuuu ccgcauaccu uauuacguag gaccacuagc aagaggcaac     1380

agggacuuug cuuggcucac acgcaauucu gaugaagcca uccggccgug gaacuucgag     1440

gaaauuguug acaaggcuuc cucagccgag gauuuuauca auaaaaugac caacuaugac     1500

cuguaccuuc ccgaggaaaa aguguugccu aagcauaguc ugcucuauga gacuuucgca     1560

gucuacaaug aacugacaaa ggugaaauuu aucgccgagg gccuuagaga uuaccaguuc     1620

cuggacagcg ggcagaagaa acagauugug acccaauugu uuaaggagaa aaggaagguc     1680

acggaaaagg acauaaucca guuccuccac aacguugaug gauaugacgg uauugagcug     1740

aaaggcaucg aaaagcaguu uaaugcuucc uuaucuaccu accacgaucu gcuaaaaauu     1800

aucaaggaca aagaguucau ggaugacagc aagaacgaag agauacucga aaauaucgug     1860

cauacacuga cuauuuuuga ggaucgugag augaucaagc agcggcuugc gcaauaugac     1920

ucauuguucg augaaaaagu aauuaaggca cugauccgaa gacacuacac cgggugggga     1980

aaacucagug ccaagcugau agacggcauu ugcgauaaac agacagguaa cacuaucuua     2040

gacuaucuga uugaugacgg gaagaauaac aggaauuuua ugcagcuuau caacgacgau     2100

ggcuuguccu ucaaggagau aacccagaaa gcucaagugg ucggaaagac agacgaugug     2160

aaacagguug ugcaggaacu cccaggcagc cccgccauca agaaagggau ucugcagucu     2220

aucaagauug ucgacgagcu agugaaagua augggacaua ccccugaauc aaucguuaua     2280

gagauggcac gcgagaacca aacuacggcc cgggguaaga agaauaguca gcagagauac     2340

aaaaggauug aagaugcucu gaagaaccuc gccagcggcc uggacuccaa uauccuuaaa     2400

gagcacccaa caaacaauau ccaauugcag aacgaucgcc uguucuuaua uuaccuccag     2460

aaugggcgag acauguacac cggaaagccc cuggauauua accagcuuuc uucguaugac     2520

aucgaucaua uugugccgca agcauuuaua aaagacgaca gccuggauaa ucgggucuug     2580

acuucccuca aggacaaccg uggcaaguca gauaaugugc cuaguaucga agucgugcag     2640

aaaagaaagg cguucuggca gcagcugcua gacucuaaac ugauuagcga gaggaaguuu     2700

aacaaucuca ccaaagcuga gcgcgguggg cuggaugaac gggacaaggu uggcuucauc     2760

aagagacaac uuguggagac aaggcagauu accaaacacg uagcccagau cuuggaugca     2820

cgauuuaaca aggaagucaa ugagaaagac aagaaaaacc gcacugugaa gauaaucaca     2880

cugaagucca acuuaguuuc aaauuuccgg aaagaauuua gacucuacaa ggugagggag     2940

auuaacgauu aucaccaugc ccacgacgcu uaccugaaug ccgucguggc aaaagcuauc     3000

cuuaagaaau aucccaagcu ggagccagaa uuuguauacg gagauuauca gaaguacgac     3060

cuaaaacguu acauuagccg cucucggaac ccuaaggagg uggaaaaagc cacggagaag     3120

uauuucuucu acuccaaucu ccugaacuuu uucaaagaag agguucauua ugcggauggc     3180

accaucguca agagagagaa uauagaauac aguaaagaca cuggggagau ugccuggaac     3240

aaggaaaagg acuucgcuac aaucaaaaag guguugagcu auccccaagu gaauaucguc     3300

aaaaagaggg agguucagac cggagguuuu ucuaaagaau ccauucugcc aaagggcaac     3360

ucagauaagu uaauaccccg aaaaacaaag gacauccucu gggagacuac caaauacggg     3420

ggauucgaua guccugugau ugcauauagc auccugcuua uugccgacau cgagaagggc     3480

aaagcuaaga agcugaaaac ggugaagacc uugguaggua uaacaaucau ggaaaaagcc     3540

gcauuugagg aaaauccaau uacuuuccuc gagaacaagg gguaccacaa uguccggaaa     3600

gagaacaucc ugugucuacc gaaguacucg cuguuugaac uugagaaugg ccgcagaagg     3660

cuccuggcgu ccgccaagga auugcagaaa ggaaacgaga uugugcugcc cguuuauuua     3720

accacacucc uguaccacuc uaagaacgug cauaaacuug augaaccuga gcaccuggag     3780

uauauccaaa agcaucggaa ugaguucaaa gacuugcuca accuggucag cgaauuuuca     3840

cagaaguacg ugcuagcuga ugccaaucug gagaaaauac agaaccuuua ugcagacaau     3900

gaacaggcug auauugaaau ccucgccaac aguuucauca aucuguugac uuuuaccgca     3960

uuaggcgccc cagcugcguu caaguuuuuc gggaaggaca uugaucgaaa aagauacacg     4020

acaguuuccg agauccugaa cgccacccuc auacaccaau cuauuacugg acuguaugag     4080

acacgcaucg accuuagcaa gcugggcgaa gauuga                               4116


<210>  3150
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3150
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggauccgc      240

uaucugcagg agauuuucag cuccgaaaug ucuaaaguag acgauucguu uuuccauaga      300

cuagaggaau cauuucucgu ggaggaggac aagaaacacg aaaggcaucc uaucuucgga      360

aacauagucg augagguggc uuaccacgaa aaguauccaa ccaucuacca ucugcggaag      420

aaacuugccg acagcacaga uaaggcagac cugcgcuuga uuuaucucgc ccuggcucac      480

augaucaaau uucgaggcca cuuccugauu gagggugauc ucaauccgga caacagugac      540

auggauaagu uauuuaucca acugguucag accuacaauc agcuuuucga ggaaaacccc      600

auaaaugcau ccagagugga cgccaaagcu auuuugucug ccaggcugag caagucccgg      660

cgucuagaga accucaucgc gcagcugccu ggggaaaaac gcaauggacu uuuuggcaac      720

cugauugcau ugucacucgg ccugacucca aauuucaaga guaacuuuga uuuagccgag      780

gacgcuaagc ugcaacucag caaagauaca uacgacgaug accuugauaa ucuguuggcc      840

cagaucgggg accaguaugc agaucuauuc cuggcugcca agaaccucuc ugacgcgauc      900

cugcuuuccg acauucugag agucaacuca gaaauaacca aagcccccuu gagcgcaagu      960

augaucaaga gguacgauga gcaucaccag gaccucacgc uguuaaaagc ucuggugcgg     1020

caacagcuac cugagaagua uaaggaaauu uucuuugauc agucuaaaaa uggauacgcc     1080

gguuauaucg acggcggggc uucccaggag gaguucuaca aguuuauaaa accaauucuc     1140

gaaaagaugg auggaacuga ggaacugcuu gccaaauuga accgagagga ccugcuccgc     1200

aagcaaagaa ccuucgauaa uggcagcauc ccccaucaga uccaccuggg ugaacuucac     1260

gcaauucuga ggcggcagga ggacuuuuau ccuuucuuga aggauaaccg cgaaaagauc     1320

gagaagauuc ucacauuuag aauaccauac uacguagggc cgcuggccag gggaaauucg     1380

cguuucgcuu ggaugacucg gaaaucugaa gagaccauca cacccuggaa cuuugaggaa     1440

guuguggaca agggcgcguc cgcacaauca uucauugagc gaaugaccaa uuuugauaaa     1500

aacuuaccua augaaaaggu ccugccaaag cauagucuac ucuaugagua cuucacggug     1560

uauaacgagc ugacuaaagu gaaguacguc acagaaggca ugagaaaacc cgaguuucuu     1620

agcgggaagc agaaagaagc caucguugac uugcuguuca agaccaauag gaaagugacu     1680

guaaagcagc ucaaggagga uuauuucaaa aagaucgaau gcuuugacuc cguggagauu     1740

ucuggagucg aggaccgcuu caacgcuagc cuggguacau accacgauuu acugaaaaua     1800

aucaaggaca aagauuuucu ugacaaugaa gagaacgaag auauuuugga ggacaucgug     1860

cucacccuga cgcuauucga agauaaggag augauugagg aacggcugaa gaaauaugcc     1920

aaucucuuug acgauaaggu uaugaaacag cugaagagaa ggcauuacac cggcuggggg     1980

cgccuuucac gaaaauugau caacggaaua cgggacaagc aaaguggcaa gacaauccug     2040

gauuucuuaa aauccgacgg uuuugcaaac cguaauuuca ugcagcucau uaacgaugac     2100

agccugacuu uuaaggaggc cauccagaaa gcucaggugu cugggcaagg ccacucacuu     2160

caugaacaga uugccaaucu ggcaggaagu ccugcgauca agaaaggcau auugcagagc     2220

gucaaggugg uagacgagcu cguuaaggug auggggcaca aaccagaaaa cauugucauc     2280

gagauggcua gagagaauca gaccacacaa aagggacaga aaaacuccag ggaacgcaug     2340

aagcggauug aggaagguau caaagagcug ggcucucaga uacuaaagga gcaccccgug     2400

gaaaauacuc agcugcaaaa cgagaaacuc uaccuguauu accuucagaa ugggagagau     2460

auguaugucg accaggaauu ggauaucaac aggcugucgg acuacgaugu ggaccauauu     2520

guuccgcaga gcuucaucaa ggaugacucc auugauaaua agaucuuaac ccgaucagac     2580

aaaaaccgcg gaaagaguga uaaugugccu ucugaggaag uagucaaaaa gaugaaaaac     2640

uauuggcggc aacuccugaa ugccaaacuu auaacacaga gaaaguuuga caaccugacc     2700

aaagcagaga ggggcggucu aagcgagcuc gacaaggccg gguucauuaa acgucagcug     2760

guggaaacuc gccaaaucac gaagcacguu gcucagaucu uggauucccg gaugaacaca     2820

aaauacgacg agaaugauaa gcugauuaga gaagugaagg ucaucaccuu aaaaucaaag     2880

cucgugagcg acuuuaggaa agauuuccag uucuauaagg uacgagagau uaacaauuac     2940

caucacgccc acgacgcaua ccugaacgcu gugguuggca cugcccuuau caaaaaguau     3000

accaagcugg aaucugaguu ugucuacgga gauuauaaag uguacgacgu gcggaagaug     3060

auagcgaaau ccgagcagga aauuggcaag gccacagcua aacgcuucuu uuauaguaau     3120

aucaugaacu ucuuuaagac cgagauuacu uuggcaaaug gggaaaucag aaagaggccc     3180

cucauagaga caaacgaaga gacgggagag aucgucuggg auaaaggucg ggacuucgcc     3240

accguucgaa aggugcugag caugccacaa gugaauauug ucaaaaagac ugaaguacag     3300

acaggcgcuc uaaccaacga gucuaucuac gccagagggu ccuuugauaa acugauuuca     3360

cgcaagcaua gguucgaaag uagcaaauau ggaggcuuug guucgccuac agugacuuac     3420

uccguucuug uggucgcaaa gucuaaggug caggacggga aaguuaagaa aaucaagacc     3480

ggcaaagagc ucauaggaau gacgcuguug gauaagcugg uguucgagaa gaaucccuua     3540

aaauuuauug aagacaaggg cuacgggaac guacagaucg acaaauguau caagcuccca     3600

aaauauagcc uguucgaguu cgaaaaugga acccgucgga ugcuugcguc agucauggcc     3660

aacaauaaca guagaggcga ucugcaaaag gcuaacgaga uguuuuugcc cgccaagcuc     3720

gugacacugc uauaccacgc acauaaaauu gaauccucua aggagcugga gcacgaggcu     3780

uauauacuug accauuacaa ugaucucuau cagcuguuga gcuacaucga aagguucgcc     3840

uccuuauaug uggacgucga gaaaaacauu ucaaagguua aagaacuguu uagcaauauc     3900

gaaaguuacu cuauuuccga gaucugcagc ucggugauaa accuccugac ucuuaccgca     3960

ucaggugccc cugcugauuu caaguuucug gggacaacua ucccacgcaa gcgauacgga     4020

ucuccgcaga guauuuuguc cagcacccua auccaccaau cuauuacggg ccuguaugag     4080

acaagaaucg accucagcca gcuggguggc gauuga                               4116


<210>  3151
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3151
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggauccgc      240

uaucugcagg agauuuucag cuccgaaaug ucuaaaguag acgauucguu uuuccauaga      300

cuagaggaau cauuucucgu ggaggaggac aagaaacacg aaaggcaucc uaucuucgga      360

aacauagucg augagguggc uuaccacgaa aaguauccaa ccaucuacca ucugcggaag      420

aaacuugccg acagcacaga uaaggcagac cugcgcuuga uuuaucucgc ccuggcucac      480

augaucaaau uucgaggcca cuuccugauu gagggugauc ucaauccgga caacagugac      540

guugauaagu uauuuaucca acuggugcag accuacaauc agcuuuucga ggaaaacccc      600

auaaaugcau ccagagucga cgccaaagcu auuuugucug ccaggcugag caagucccgg      660

cgucuagaga accucaucgc gcagcugccu ggggaaaaac gcaauggacu uuuuggcaac      720

cugauugcau ugucacucgg ccugacucca aauuucaaga guaacuuuga uuuagccgag      780

gacgcuaagc ugcaacucag caaagauaca uacgacgaug accuugauaa ucuguuggcc      840

cagaucgggg accaguaugc agaucuauuc cuggcugcca agaaccucuc ugacgcgauc      900

cugcuuuccg acauucugag agugaacuca gaaauaacca aagcccccuu gagcgcaagu      960

augaucaaga gguacgauga gcaucaccag gaccucacgc uguuaaaagc ucugguacgg     1020

caacagcuac cugagaagua uaaggaaauu uucuuugauc agucuaaaaa uggauacgcc     1080

gguuauaucg acggcggggc uucccaggag gaguucuaca aguuuauaaa accaauucuc     1140

gagaagaugg auggaacuga agaacugcuu gccaaauuga accgagagga ccugcuccgc     1200

aagcaaagaa ccuucgauaa uggcagcauc ccccaucaga uccaccuggg ugaacuucac     1260

gcaauucuga ggcggcagga ggacuuuuau ccuuucuuga aggauaaccg cgagaaaauc     1320

gaaaagauuc ucacauuuag aauaccauac uacguugggc cgcuggccag gggaaauucg     1380

cguuucgcuu ggaugacucg gaaaucugaa gagaccauca cacccuggaa cuuugaggaa     1440

guggucgaca agggcgcguc cgcacaauca uucauugagc gaaugaccaa uuuugauaaa     1500

aacuuaccua augaaaaggu gcugccaaag cauagucuac ucuaugagua cuucacggug     1560

uauaacgagc ugacuaaagu caaguacguu acagaaggca ugagaaaacc cgccuuucuu     1620

agcggggagc agaagaaagc uaucguggac uugcuguuca agaccaauag gaaaguaacu     1680

gugaagcagc ucaaggaaga uuauuucaaa aagaucgagu gcuuugacuc cgucgaaauu     1740

ucuggagugg aggaccgcuu caacgccagc cuggguacau accacgauuu acugaaaaua     1800

aucaaggaca aagauuuucu ugacaaugag gaaaacgagg auauuuugga agacaucguu     1860

cucacccuga cgcuauucga ggauaaggaa augauugagg agcggcugaa gaccuaugca     1920

caucucuuug acgauaaagu gaugaagcag cugaaaagaa ggcacuacac aggcuggggg     1980

cgccuuucac gaaaguugau caauggaaua cgggacaaac aaaguggcaa gacuauccug     2040

gauuucuuaa aguccgacgg uuuugccaac cguaacuuca uucagcucau ccaugaugac     2100

agccugaccu uuaaagaagc uauucagaag gcccaggucu cugggcaagg ccacucacuu     2160

cacgagcaga ucgcaaaucu ggcgggaagu ccugcuauua aaaagggcau auugcagagc     2220

gugaaaguag uugacgaacu cgugaagguc auggggcaua agccagagaa caucgugauu     2280

gaaauggcca gagagaauca gacaacucaa aaaggacaga agaacuccag ggagcgcaug     2340

aaacggaucg aagaggguau aaaggaacug ggcucucaga uccuaaaaga gcaccccguc     2400

gagaauaccc agcugcaaaa cgaaaagcuc uaccuguauu accuucagaa ugggagagau     2460

auguaugugg accaggaguu ggauauuaac aggcugucgg acuacgaugu ugaccauauc     2520

gugccgcaga gcuucauuaa agaugacucc aucgauaaua agguauuaac acgaucagac     2580

aagaaccgcg gaaaaaguga uaaugucccu ucugaagagg ugguuaagaa aaugaagaac     2640

uauuggcggc aacuccugaa ugcaaaacuu auaacccaga gaaaguuuga caaccugacu     2700

aaggccgaaa ggggcggucu aagcgagcuc gacaaagcug gguucauuaa gcgucagcug     2760

guggagacgc gccaaaucac aaaacacguc gcccagaucu uggauucccg gaugaauacc     2820

aaguacgacg aaaacgauaa acugauuaga gaggugaagg uaaucacuuu aaagucaaaa     2880

cucgugagcg acuuuaggaa ggauuuccag uucuauaaag uucgagaaau uaacaauuac     2940

caccaugcac acgacgcuua ccugaacgcc gucgugggca ccgcgcuuau aaagaaauau     3000

cccaagcugg agucugaauu uguguacgga gauuauaagg ucuacgacgu ucggaaaaug     3060

aucgccaagu ccgagcagga gauuggcaaa gcuacagcaa agcgcuucuu uuauaguaau     3120

aucaugaacu ucuuuaaaac cgaaauuacu uuggccaaug gggagaucag aaagaggcca     3180

cucauagaaa caaacgagga aacgggagag aucguguggg auaagggucg ggacuucgcu     3240

accgugcgaa aaguacugag caugccucaa gucaauauug ugaagaaaac ugagguucag     3300

acaggcgccc uaaccaacga aucuaucuac gcaagagggu ccuuugauaa gcugauuuca     3360

cgcaaacaua gguucgagag uagcaaguau ggaggcuuug guucgcccac agugacuuac     3420

uccguccuug ugguugcgaa aucuaaggug caggacggga aggucaaaaa gaucaaaacc     3480

ggcaaggaac ucauaggaau uacgcuguug gauaaacugg uauucgagaa gaauccauua     3540

aaguuuaucg aggacaaagg cuacgggaac gugcagaucg acaaguguau uaaacucccc     3600

aaguauagcc uguucgaguu cgaaaaugga acccgucgga ugcuugccuc agugauggcu     3660

aacaauaaca guagaggcga ucugcaaaaa gccaaugaaa uguuuuugcc ugcaaagcuc     3720

gucacacugc uauaccacgc ucauaagaua gaguccucua aagaacugga gcacgaggcc     3780

uauauccuug accacuacaa cgaucucuau cagcuguuga gcuacauuga aagguucgca     3840

uccuuauaug uugacgugga gaagaacauc ucaaaaguaa aggaacuguu uagcaauauu     3900

gagaguuacu cuaucuccga aauaugcagc ucggugauca accuccugac ucuuaccgcc     3960

ucaggugcuc cagcggauuu caaauuucug gggacaacua uuccgcgcaa gcgauacgga     4020

ucuccccaga guaucuuguc cagcacccua auucaucaau cuaucacggg ccuguaugag     4080

acaagaauag accucagcca gcuggguggc gauuga                               4116


<210>  3152
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3152
auggagaaca agaauuacag caucggccug gacauuggga ccaacuccgu gggauggucu       60

gucauaacag augacuauaa aguucccuca aagaaaauga aggugcucgg uaauacugau      120

aaacacuuca ucaagaagaa ccuuauuggc gcccuguugu uugacgaagg gacgaccgcu      180

gaggcaagaa gguuaaaacg gacagcccgc cgacguuaca cuagaaggaa gaaucggcug      240

cgcuaucuac aggaaaucuu cagugaggaa auuggaaaag uagauagcuc cuuuuuccau      300

agacucgacg auucuuuucu gaucccugag gacaagaggg gcucgaaaua cccaauauuc      360

gcgacccuug cugaggaaaa gaaguaucac aaacaauuuc cgacaaucua ccaucugcgg      420

aagcaguugg ccgauucaaa agagaagacc gaccuccgcc ugauuuaucu ggcacucgcc      480

cacaugauca aauaccgagg ucauuucuua uacgaagagg cuuuugauau uaagaacaau      540

gacauccaga aaauauucaa cgaguuuauu agcaucuaua auaacacuuu cgaagggaau      600

agucuguccg gacagaacgu gcaagucgag gcaauuuuua cagauaagau cucuaagagc      660

gccaaaagag aaagggugcu uaaguuguuc cccgacgaga aauccaccgg ccuguucuca      720

gaauuucuaa agcucaucgu uggcaaucag gcugacuuca aaaagcacuu ugaucuggag      780

gagaaggccc cucuucaguu cagucgggac acguacgaug aagaccugga gaacuugcuc      840

gggcagauug gagaugacuu ugcggaucug uucuuagugg ccaaaaagcu guaugacgca      900

auacuccuua gcgguauccu gacugucacc aauccaucua caaaagcucc cuuguccgcc      960

ucaaugauug aacguuacga gaaccaccaa aaggaucuag caagccugaa acaguuuauc     1020

aagaacaauc ucccugaaaa guaugacgag guguucagug aucagucuga ggacggcuac     1080

gcuggguaua uagacggaaa aacuacccag gaaacauuuu acaaguacau uaaaaaccug     1140

cuuuccaagu ucgagggcgc cgauuauuuu cuggacaaaa ucgaacgcga ggauuucuug     1200

agaaagcaaa ggaccuuuga caaugguagc aucccacauc agauucaccu ccaggaaaug     1260

aacgcgaucc ugcggcgaca aggggagcau uaccccuucu uaaaggagaa uaaagaaaag     1320

auugagaaaa uacugacguu ccgcaucccu uauuacguag gaccacuagc cagaggcaac     1380

agggauuuug cauggcucac ucgcaauucg gaccaggcua uucggccgug gaacuucgaa     1440

gagaucguug auaaggccuc uuccgcugag gacuuuauca auaaaaugac aaacuaugau     1500

cuguaccuuc ccgaagagaa gguguugccu aaacacucac ugcucuauga aaccuucgcc     1560

gucuacaaug agcugacuaa ggugaaguuu auugcagaag gccuuagaga cuaccaguuc     1620

cuggauagug ggcagaaaaa gcaaauagug acacaguugu uuaaagagaa gaggaaaguc     1680

accgagaagg acaucauuca guuccuccac aacguugaug gauaugacgg uaucgaacug     1740

aagggcauug agaaacaguu uaaugccagc uuauccacgu accaugaccu gcuaaagauc     1800

auaaaagaua aggcuuucau ggacgaugcg aaaaacgaag caauccucga gaauauugug     1860

cacacccuga caaucuuuga agaccgugag augauuaagc aacggcuugc ccaguaugau     1920

ucuuuguucg acgagaaggu aaucaaagcu cugacucgaa gacauuacac cgggugggga     1980

aagcucagcg ccaaacugau aaacggcauu ucagauaagc agacagguaa cacuaucuua     2040

gacuaucuga uugaugacgg ggaaaucaau aggaacuuua ugcagcuuau aaaugaugac     2100

ggcuugaguu ucaaagagau cauucaaaag gcacaggugg ucggaaagac caacgaugug     2160

aaacagguug ugcaggaacu cccaggcucc cccgccauca agaaagggau ucugcaaagc     2220

aucaagauag ucgacgagcu agugaaagua augggacacg cuccugaauc uauuguuauc     2280

gagauggccc gcgagaauca gacaaccgca cgggguaaga aaaacucaca gcaaagauac     2340

aagaggaucg aagacagucu gaagauucuc gcgagcggcc uuaauuccaa aauccugaag     2400

gagcauccaa cugauaacau ucaguugcag aaugaccgcc uguucuuaua uuaccuccag     2460

aacgggaaag auauguacac gggagaagcu cuggacauaa aucaacuuuc uucgugcgau     2520

aucgaccaca uuauccccca ggccuuuauu aaggaugaca gccuggauaa ccgaguguug     2580

acauccucaa aagacaaucg gggcaagagu gauaacgucc cgucucucga gguggucgac     2640

aagaugaaag uguucuggca gcagcugcua gacagcaagc ugaucuccua ucguaaauuu     2700

aauaaccuca ccaaggcaga gagagguggg cugaacgaac uugauaaagu uggcuucaua     2760

aagaggcaau ugguggagac ucgccagauc accaagcacg uagcccagau ucuggacgcu     2820

cgguuuaaua aagaagucac agagaaggau aaaaagaaca gaaccgugaa aaucauuacu     2880

uuaaagucaa aucucguuag caacuucagg aaggaauuug agcuguacaa agugcgagag     2940

aucaaugacu aucaucacgc ccaugaugca uaccuuaacg cugucguggc caaggcgaua     3000

cugaaaaagu auccuaaacu agaacccgag uucguauacg gagacuauca gaaguacgau     3060

cucaagcgcu acauuucucg guccaaagac ccaaaggaaa ucgagaaagc cacagaaaag     3120

uacuuuuucu auaguaaucu guugaacuuu uucaaagagg aggugcacua ugcugauggc     3180

acgaucguua agagagaaaa uauugaguac agcaaagaca ccggggaaau agcauggaac     3240

aaggagaagg auuucgccac uaucaaaaag guccugucuu uaccucaagu gaauauugug     3300

aaaaagaggg aaguccagac aggagguuuu uccaaagagu caauccuccc caagggcaac     3360

agugacaagc ugauuccacg uaaaaccaag gauauccuuu gggacacaac uaaauauggg     3420

ggauucgaca gccccguuau agcuuacucg auccuguuga uugccgauau cgagaagggc     3480

aaagcaaaga agcucaaaac cgugaagacg cuggugggua uuaccaucau ggaaaaagac     3540

gcguuugaga agaauccuau agccuuccua gaaaacaaag gguaucacaa uguauguaag     3600

gagaacauuc ugugccuucc aaaguacucc cucuuugagc uggaaaacgg ccgccggaga     3660

uugcuggcuu cugccaaaga guuacagaag uguaaugaaa ucguccuccc gguguaccug     3720

acaacucuuc uguaucauag caaaaacguu cacaaguugg augagcccgg acaucucgaa     3780

uacauccaaa aacacaggaa ugaguucaag gaccugcuaa accugguguc agaguuuagu     3840

cagaaauaug uccuugcaga ugcuaaucuc gaaaagauua agaaccugua cgccgacaau     3900

gagcaggcag auaucgaaau auuggccaac uccuucauua auuuacugac cuuuacagcu     3960

cucggcgcgc cugccgcauu caaauuuuuc gggaaggacg uggaucgaaa acgguauacu     4020

accguuucug agauccugaa cgcuacgcuu auucaucaga gcaucacagg acuguacgaa     4080

acccgcauag acuuguccaa gcuaggcgag gauuga                               4116


<210>  3153
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3153
augaagguga acaauuacca caucggccug gacauuggga ccagcuccau aggauggguc       60

gccaucggug aggauggcaa accccucaga auuaagggga aaacagcuau cggagcaagg      120

cuuuuccagg aaggcaaccc ugccgcggac cggcgcaugu uucgaacuac gcguagaagg      180

cugucucggc gcaaguggag auugaaauua cuggaggaaa uuuucgaucc auauaucacc      240

ccgguugacu caacauuuuu cgcuaggcua aagcaaagua aucucagccc caaggauucc      300

cggaaagagu uuaaggguuc uaugcuguuc ccugaccuua cugauaugca guaccauaaa      360

gacuauccaa ccauauacca ucugcgccac gccuugauga cacaggauga aaaguuugac      420

auccgaaugg uguaucucgc aauucaccau aucguaaaau acagagggaa cuuccuaaau      480

ucgacccccg uggauucauu uaaggccagc aaggucaacu ucguggacca guuuaaaaag      540

cugaaugagc ucuauacugc uauuaacccu gaggaaaguu uccaaaucaa uuuagcaaac      600

uccgaggaua uaggacacca guuucuggac ccaucuauua ggaaauucga caagaaaaag      660

cagaucccca aaauuguucc ugugagcguc gaugacaagg ccacagauaa gaucaauggc      720

aaaaucgcuu ccgaaauuau aaacgccauc cuuggcuaca agucaaaauu ggacguggua      780

guucagugca ccccagugga uaguaagagc ugggcgcuga aauucgacga ggaggauauu      840

gacgcaaagc uacaaaagau ccucccggaa auggaugaga aucagcaguc uauaauugcc      900

auccugcaga accuuuacuc ccaagucacg cugaaucaga ucgugcccaa cgggauguca      960

uugagcgaaa guaugauuga gaaauauaac gaccaccaug aucaccucaa gcuguacaaa     1020

aagaucauug accaguuagc ugacccuaaa aagaaggccg cacugaaaaa ggcuuauucu     1080

caauacgugg gagaugacgg uaaagucaua gaacaggccg auuuuugguc cagcguuaag     1140

aaaaaucucg acgauucgga ccuuucuaag cagaucaugg aucugauuga cgcggagaag     1200

uucaugccaa aacagcggac uucccaaaac ggcgugaucc cccaucaguu gcaccagcgu     1260

gagcuagaug aaaucauuga gcaccaguca aaguauuacc cuuggcuggc cgaaauaaau     1320

ccaaacaaac augaccucca ccuggcaaag uauaaaaucg aggaacuugu agcuuuucgc     1380

gugcccuacu acgucgggcc gaugauuacc ccugaugacc aagccaagag ugcugagaca     1440

guguucagcu ggauggagag aaaaggaaag gaagccggcc agaucacucc cuggaauuuu     1500

gaugagaagg uugacaggaa cgcauccgcc aaucgguuca uuaaacgaau gaccacaaag     1560

gacaccuauc ugaucgguga agauguguug ccagacgagu cucuccugua cgaaaaauuu     1620

aaggucuuaa acgagcugaa uauggugcgc guaaacggga aacuacucaa gguugcugau     1680

aagcaggcga uauuccagga ccuguuugag aauuauaaac auaucagcgu gaagaaacuu     1740

caaaacuaca uuaagucaaa aacgggauug ccuagugauc ccgaaaucuc cggccugagc     1800

gacccagagu auuucaauaa cucucucggc acuuacaaug auuuuaagaa gcuguucggg     1860

aacaaagucg acgaacccga ucuucaggac gauuuugaga agauugugga guggucaaca     1920

gucuucgaag acaaaagaau ccugagggag aaguugaacg aaauaaccug gcucagugau     1980

cagcagaaag acgugcugga gagcucccgg uaucaaggau ggggucgcuu aucuaagaag     2040

cugcuaacug gcauuguuaa ugaccagggg gaaagaauca uugaugagcu cuggaacaca     2100

aauaaaaacu ucaugcagau ccaaucggac aaugauuuug caaagaggau acacgaggcc     2160

aacgcugacc agaugaaagc cguggaugua gaagacgucc uggcagaugc cuacaccagc     2220

ccucagaaua agaaagcuau ccgucaggug guuaaggugg ucgacgauau ucaaaaagcc     2280

augggaggcg uggcaccaaa guacaucucc auugaguuca cgcggucaga agaccgaaac     2340

ccgagaagga ccaucagucg ccagcggcag cuugagaaua cauugaagga uacugcgaaa     2400

ucucuggcua agagcauaaa ccccgaacuc cuguccgagu uagacaaugc cgcaaaauca     2460

aagaaagguc ugauugacag acuuuauuug uacuuuaccc agcucgggaa ggauaucuau     2520

acaggcaagc cuaucaacau ugacgagauc agcacuuacg auauugacca uauacugcca     2580

caagccuucg uaaaagauga cucucuaaau aacagggugc ugguuuccaa ggcuaucaau     2640

aacggaaaaa gugauaacgu ccccgugcag cucuuuggcg ccaagauggg gcacuucugg     2700

aaacagcugg cagaagcugg acuuauuagc aagcgcaagu ugaaaaaucu gcagaccgac     2760

ccugauacaa ucucuaagua ugccaugcac gguuuuauuc gacggcaauu aguggagacc     2820

ucccagguca ucaaacucgu ugcgaacaua cugggcgaca aguaccguaa ugaugacacu     2880

aaaaucauug aaaucacggc cagaaugaac caucagauga gggaugaguu cggguuuauu     2940

aagaaucgcg aaaucaacga cuaucaccau gcuuucgacg cauaccuuac agccuuucug     3000

ggacgguacu uguaucacag auacauaaag cucaggccau auuucgugua cggcgauuuu     3060

aaaaaguuca aagaggacaa ggugaccaug cgaaauuuca acuuucugca cgaucuaacu     3120

gacgauaccc aagagaaaau ugcugacgcc gaaacaggug agguaaucug ggaucgcgaa     3180

aauucaaucc agcagcugaa ggacgucuau cauuacaagu ucaugcucau uagucacgag     3240

guguauaccc ugagaggggc aauguuuaac cagacuguuu accccgcgag cgaugccggc     3300

aaacggaaac uuauaccgau caaagcugac aggccuauua auguguacgg aggcuauucg     3360

ggguccgccg augcauacau ggcuaucguc cguauucaua acaagaaagg agacaaguau     3420

cgcgugguug gugugccaau gcgggccaga gauagguugg acgcagccaa aaagguaucu     3480

gacgcugauu acgaccgagc gcugaaggau gucuuaacac cccaacucac gaaaaccaag     3540

aaaagccgga agacuggcga gaucacacag gugguggaag acuucgagau aguccugggg     3600

aaaguuaugu aucgccagcu uaugaucgau ggagacaaga aguuuaugcu gggcucaagu     3660

accuaccagu auaaugccaa acaacuggug cucuccgauc agucuguaaa gacacuggca     3720

agcaaaggua gauuggaccc ucugcaggaa uccauggauu acaacaaugu guacacugag     3780

auuuuagaca aggucaacca guauuucuca cucuacgaua ugaacaaauu uaggcacaag     3840

cugaaucuug gguucagcaa guuuaucagu uuccccaacc auaauguguu ugacggcaac     3900

accaaggcuu cuuccggaaa gcgggaaauu cuggaggagg uuuugaaugg ccuccacgcc     3960

aacccaacgu ucgggaaucu gaaagacauc ggaauaacca caccuuucgg ccaacuacag     4020

cagcccaacg guauucugcu uagcgaugaa gccaagaucc gauaucaguc gccaacuggg     4080

cucuuugaga gaaccguguc acugaaagac uuguga                               4116


<210>  3154
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3154
augaagguga acaauuacca caucggccug gacauuggga ccagcuccau aggauggguc       60

gccaucggua aagauggcaa gccccucaga guuaaaggga agacagcuau uggagcaagg      120

cuuuuccagg agggcaaccc ugccgcggac cggcgcaugu uucgaacuac gcguagaagg      180

cugucucggc gcaaauggag auugaaguua cuggaagaga ucuucgaucc auauauuacc      240

ccgguggacu caacauuuuu cgcuaggcua aagcaaagua aucucagccc caaagauucc      300

cggaaggaau uuaaagguuc uaugcuguuc ccugaccuua cugauaugca guaccauaag      360

aacuauccaa ccaucuacca ccuucgccau gccuugauga cacaggacaa aaaguuugau      420

auacgaaugg uauaucucgc aauccaccau auugugaagu acagagggaa uuuccugaac      480

ucgacccccg ucgacucauu uaaagccagc aagguggauu ucguugacca guuuaaaaag      540

cugaaugagc ucuaugcugc aaucaacccu gaagagaguu ucaaaauuaa uuuagccaac      600

uccgaggaua ucggacacca auuucuggac ccaucuauaa ggaaguucga caaaaagaag      660

cagauuccca aaaucgugcc ugucaugaug aaugauaagg ugacugaccg gcuuaacggc      720

aaaauugcua gcgaaaucau ccacgccauu uugggcuaca aggcgaaacu ggauguaguu      780

cuacagugca caccagugga cuccaagccg ugggcacuca aguucgauga cgaggauaua      840

gacgccaaac uggaaaagau ccuucccgag auggaugaga aucagcaauc aauugucgcu      900

auccugcaga acuuguacag ucaggugacc cucaaucaga uagugccuaa cgggaugagc      960

cugucugaau ccaugauuga gaaauauaac gaccaucacg aucauuuaaa gcuguacaaa     1020

aagcucaucg accaacuugc cgacccaaag aaaaaggcag uccugaaaaa ggcuuauuca     1080

caguacguug gagaugacgg uaaagugauc gaacaggccg aguuuuggag caguguaaag     1140

aagaauuugg augacucuga acuauccaaa caaauuaugg aucugaucga cgcggagaag     1200

uucaugccca aacagcguac gagccagaac ggcgugauuc cucaccagcu ccaccaacgc     1260

gagcuggaug aaauaaucga gcaucagucg aaguauuacc cauggcuugu cgaaauuaau     1320

cccaacaaac acgaccugca uuuggccaag uauaaaaucg agcagcucgu ggcauuuaga     1380

guuccguacu acguggggcc uaugaucacu cccaaggauc aggcugaauc ugccgagacc     1440

gucuucuccu ggauggagag gaagggaaca gaaacuggcc aaauuacccc auggaauuuu     1500

gacgagaaag uggaucggaa ggcuucagcc aaccgauuca uaaaacgcau gacaaccaag     1560

gacacguauc ugaucgguga agauguauua ccugacgaga gucugcuaua cgaaaaauuu     1620

aagguucuca augagcugaa cauggugaga gucaauggga agcuguugaa aguggcagac     1680

aagcaggcca uuuuccagga ucuguuugag aacuauaaac acgucagcgu gaagaaacuc     1740

cagaauuaca ucaaggcuaa gacuggacug cccuccgacc cagaaauuuc uggccuuagc     1800

gaucccgagc auuucaacaa uucacugggc acauauaacg acuuuaaaaa guuguucggg     1860

aguaaaguug augaaccuga ccuccaagau gacuuugaga agaucgugga gugguccacc     1920

guauucgaag auaaaaagau acugagggag aaguuaaaug aaaucacuug gcugagcgac     1980

cagcagaaag auguccuaga gucuucacgg uaccagggau ggggucgccu caguaagaaa     2040

cugcuuacag gcauugugaa cgaccaaggg gaaagaauca uugauaaguu guggaacacc     2100

aauaaaaacu ucaugcagau ccagagcgac gacgauuuug cgaagaggau acacgaggca     2160

aaugccgacc aaaugcaggc uguugaugug gaggacgucc uggccgaugc auauacgucc     2220

ccacagaaca aaaaggccau ucgucaggug guaaaggugg uugacgauau ccaaaaagcu     2280

augggaggcg ucgccccgaa guacauuucu aucgaguuca cccggucgga agaccgaaau     2340

cccagaagga caauaagccg ccagcggcag cucgaaaaca cucugaaaga uaccgcaaag     2400

uccuuagcga aaucaaucaa uccugagcug cuuagugaau uggacaacgc ugccaagucu     2460

aagaaagguc ucacagauag acuguaccua uauuuuacuc agcuggggaa ggacauuuac     2520

accggcgagc caaucaauau ugacgagcuc aacaaauaug auaucgacca cauacugccc     2580

caagcauuca uuaaggauaa uagccuugac aacagggugu uggugcugac agccgucaau     2640

aacggaaaau ccgauaaugu uccuuuacgc auguuuggcg cuaagauggg gcauuucugg     2700

aagcagcucg ccgaagcagg acugaucuca aaacgaaaac uuaagaaccu gcagaccgac     2760

ccagauacua ucagcaagua cgcuaugcac gguuuuauuc ggcgucaguu gguggagacg     2820

ucucaaguga ucaaacucgu agccaacauu cugggcgaca aguauagaaa ugaugacaca     2880

aagauaaucg aaauuaccgc gaggaugaac caucagaugc gcgaugaguu cggguuuauc     2940

aaaaaucggg aaauuaacga cuaccaccac gccuucgaug cuuaucuaac ugcauuucug     3000

ggaagauacc ucuaccauag guauaucaag cugcgacccu acuucgucua uggcgacuuu     3060

aaaaaguucc gcgaggacaa agugaccaug cggaauuuca acuuucuuca cgauuugaca     3120

gacgauaccc aggagaagau agccgacgcu gaaacuggug agguuaucug ggauagagaa     3180

aauuccauuc aacagcugaa ggacguguac cauuauaaau ucauguuaau cagucacgag     3240

gucuacacac uccguggggc cauguuuaac cagacggugu auccggcaag cgaugcgggc     3300

aagaggaaac ugauuccugu uaaggccgac cgcccaguga auguauacgg aggcuacucu     3360

ggguccgcug augccuauau ggcaaucguc cggauacaua acaaaaaggg agacaaauac     3420

agaguggugg guguccccau gagggcucuu gaucgacugg acgccgcaaa gaauguuuca     3480

gaugccgacu ucgaccgggc ucuaaaggau gugcucgcgc cucagcugac caaaacuaag     3540

aaaagucgca agacaggcga gauuacccaa guaaucgaag acuuugagau cguguugggg     3600

aaagucaugu auagacagcu gaugauugau ggagacaaga aguucauguu aggcagcucg     3660

acauaccagu auaacgccaa acagcucgug cuguccgauc aaucuguuaa gacucuugca     3720

agcaaaggua ggcuggaccc cuugcaggaa ucaauggauu acaauaacgu guauaccgag     3780

auacucgaca aggucaauca guacuuuagu cuguacgaua ugaacaaauu ccggcacaag     3840

cuaaaccugg gguuuuccaa guucaucucu uuuccaaauc acaacgugcu ugacggcaau     3900

acgaaaguua gcuccggaaa gcgagaaauu cuccaggaga uccugaacgg cuugcaugcu     3960

aauccuaccu ucgggaaccu gaaagaugug ggaauuacaa cucccuucgg ccaauuacag     4020

cagccaaaug guauccuccu gucagacgag accaagauaa gauaucagag cccgacaggg     4080

cuuuuugaac gcacugucag ucugaaagac uuguga                               4116


<210>  3155
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3155
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

auaacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacugacaga      120

cacucaauca agaagaaccu uauuggcgcu cuguuguuug auagugggga gacggcagaa      180

gccaccaggu uaaaacggac agcgcgccga cguuacacua gaaggaagaa ucggaucugc      240

uaucugcagg agauuuucag caacgaaaug gcuaaaguag acgauuccuu uuuccaucgc      300

cuagaggaau cuuuucucgu ggaggaggac aagaaacacg aaagacaucc uaucuucgga      360

aauauagucg augagguggc cuaccacgaa aaguauccaa ccaucuacca ucugaggaag      420

aaacuugcag acucgacaga uaaggccgac cugcgguuga uuuaucucgc ucuggcacac      480

augaucaaau uucgcggcca cuuccugauu gagggugauc ucaacccgga caauucagac      540

guugauaagu uauuuaucca acuggugcag accuacaacc agcuuuucga ggaaaauccc      600

auaaacgcca gccgagucga cgcuaaagcc auuuugagug cgagacuguc caagucuagg      660

cggcuagaga aucucaucgc acagcugccu ggggaaaaga agaacggacu uuuuggcaau      720

cugauugccu ugagccucgg ccugacucca aacuucaagu ccaauuuuga uuuagcugag      780

gacgccaaac ugcaacucuc aaaggauaca uacgacgaug accuugauaa ccuguuggca      840

cagaucgggg accaguaugc ugaucuauuc cuggccgcga aaaaccucag ugacgccacc      900

cugcuuagcg acauccugcg uguaaauucu gaaauuacga aggcacccuu guccgcuuca      960

augauaaaac gcuacgauga gcaucaccag gaccucacuc uguuaaaggc ccuggugaga     1020

caacagcuac cugagaagua uaaagaaauc uucuuugauc agagcaagaa cggauacgcu     1080

gguuauauug acggcggggc cagucaggag gaguucuaca aauuuaucaa gccaauacuc     1140

gagaaaaugg auggaaccga agaacugcuu gcaaaguuga auagggagga ccugcuccgg     1200

aagcaacgaa cauucgauaa cggcucuauu ccccaucaga uccaccuggg ugaacuucac     1260

gccauccugc gcagacagga ggacuuuuau ccuuucuuga aagauaauag ggaaaagauu     1320

gagaaaaucc ucacuuuucg gauuccauac uacguugggc cgcuggcucg cggaaacucc     1380

agauucgcgu ggaugacccg uaagagcgaa gagacaauaa cccccuggaa uuuugaggaa     1440

guggucgaca aaggcgcauc ggcccaaucu uucaucgaga ggaugacgaa cuuugauaag     1500

aauuuaccua acgaaaaggu gcugccaaaa cauucccuac ucuaugagua cuucacugug     1560

uauaaugagc ugacaaaggu caaauacguu accgaaggca ugcggaagcc cgcuuuucuu     1620

ucaggggagc agaaaaaggc cauuguggac uugcuguuca aaacuaaccg aaagguaaca     1680

gugaagcagc ucaaagaaga uuauuucaag aaaaucgagu guuuugacag ugucgaaauc     1740

agcggagugg aggacagauu caaugcaucc cuggguaccu accacgauuu acugaagauu     1800

auaaaagaca aggauuuucu ugacaacgag gaaaaugagg auaucuugga agacauuguu     1860

cucacgcuga cccuauucga ggauaaggaa augaucgagg agaggcugaa aacauaugcc     1920

caucucuuug acgauaaggu gaugaaacag cugaagcgcc ggcacuacac uggcuggggg     1980

agacuuucua ggaaauugau uaacggaauc cgcgacaagc aaagcggcaa gaccauacug     2040

gauuucuuaa aaucagacgg uuuugcuaac cgaaauuuca uccagcucau ucaugaugac     2100

agucugacau uuaaggaagc cauccagaaa gcacaggucu ccgggcaagg ccacagccuu     2160

cacgagcaga uugcgaaccu ggcuggaucu ccugccauca agaaaggcau auugcaguca     2220

gugaagguag uugacgaacu cgugaagguc auggggcaua aaccagagaa uauugugauc     2280

gaaauggcac gggagaacca gacuacccaa aagggacaga aaaauagucg ugagagaaug     2340

aagaggauug aagaggguau caaagaacug ggcagccaga uacuaaagga gcaccccguc     2400

gagaacacac agcugcaaaa ugaaaaacuc uaccuguauu accuucagaa cgggcgcgau     2460

auguaugugg accaggaguu ggauaucaau cggcuguccg acuacgaugu ugaccauauu     2520

gugccgcaau cuuucaucaa ggaugacucg auugauaaca agguauuaac cagaagcgac     2580

aaaaauaggg gaaaguccga ugacgucccu ucagaagagg ugguuaaaaa gaugaaaaac     2640

uauuggcgac agcuccugaa ugccaaacuu aucacucagc gcaaguuuga caaccugacg     2700

aaagcugaac ggggcggucu aagugagcuc gauaaggccg gguucauaaa aagacagcug     2760

guggagacaa ggcaaauuac caagcacguc gcacagaucu uggacucucg uaugaauacu     2820

aaauacgaug aaaacgacaa gcugauccgc gaggugaagg uaauuaccuu aaaaagcaag     2880

cucguguccg auuuucggaa agacuuccag uucuauaagg uuagagaaau caacaauuac     2940

caccaugcuc acgaugccua ccugaacgcg gucgugggca cagcccuuau uaaaaaguau     3000

cccaagcugg agucagaauu uguguacgga gacuauaaag ucuacgaugu uaggaagaug     3060

auagcuaaaa gcgagcagga gaucggcaag gcaaccgcca aacgauucuu uuauucuaau     3120

auuaugaacu ucuuuaagac ugaaaucaca uuggcuaaug gggagauucg gaagcgccca     3180

cucaucgaaa cgaacgagga aaccggagag auaguguggg acaaagguag agauuucgcc     3240

acugugagga agguacuguc caugccucaa gucaauaucg ugaaaaagac agagguucag     3300

accggcgcac uaacaaacga aaguauuuac gcgcggggga gcuuugacaa acugaucucu     3360

cgaaagcaua gauucgaguc cucaaaauau ggaggcuuug guagucccac ugugaccuac     3420

agcguccuug ugguugccaa gucgaaggug caggauggga aaguaaagaa aauuaagacg     3480

ggcaaagaac ucaucggaau aacccuguug gacaagcugg ucuucgagaa gaauccauua     3540

aaauuuauug aggacaaggg cuacgggaac gugcagaucg auaaaugcau caagcucccc     3600

aaauauuccc uguucgaguu cgaaaaugga acacgcagga ugcuugcuuc ugugauggcc     3660

aacaauaaca gccguggcga ccugcaaaag gcaaaugaaa uguuuuugcc ugcuaagcuc     3720

gucacucugc uauaccacgc ccauaaaauu gagucaagua aggaacugga gcacgaggca     3780

uauauacuug aucacuacaa cgaccucuau cagcuguugu ccuacaucga acgguucgcc     3840

ucuuuauaug uugaugugga gaaaaacauu agcaagguaa aagaacuguu uuccaauauc     3900

gagucauaca gcauuaguga aaucuguucu uccgugauaa accuccugac ccuuacagcu     3960

agcggugcgc cagccgacuu caaguuucug gggacuacca ucccgagaaa gagguacgga     4020

ucgccccagu caauuuuguc uaguacgcua auccaucaau ccauuacagg ccuguaugag     4080

acccgcaucg aucucagcca gcuggguucu gacuga                               4116


<210>  3156
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3156
augaacaaug aguacuucgu gggcuuugac aucgggacca acagcgucgg agccgcuguu       60

acagaugaaa aguaucacgu gcugaaauuc aaggguaaug caauguggac uuccgccgua      120

uuugagucug cgcagcaauc agcugacaga aggcucaacc ggacggcccg ccgacgucuu      180

gauagaaguc agcagaggcg ggaccuguug caggaauuaa uugcacccgc cauaaccccu      240

guggauccac gcuucuuuga cagaaucagg gagagcgcuc ugugggcaga agauaaaaca      300

acuggcuccc gguucaccua cuuuucugac gagggguaua cagauaagca auaccauaaa      360

gaguauccga ccauucacca ucuaaucugc ucgcucauga augaccccgc cccucacgau      420

ccacgccugg ucuacauugc uuguucauau augcuuacuc aucgaggaca cuuccugaac      480

gaagccgaca aggagaagau cgaugacgug uuggacauaa gaaggguuuu uaaugaacuc      540

ugcagcuggu ucacaaguua cggcaucgag gugcccuuuu ccuguuuccc ugaggaauuu      600

gguaacgucc ugaccgcgaa ucggggagug caggaucgua cgcgcgcauu cuacgaccuc      660

cuguucgccg gaaagaagcc aaaacccucu gaggaugacc agguagauau ugacguuuua      720

aucaagcuga uuagcggcgg caaaaucgaa cuuuccaagu uguuucugaa cgaggaauau      780

ucagagcuag agaauaacaa gauacagcuc agugaugcug gguucgacga aaaagagccu      840

gaacugagaa gcgagcuugg agaugacuuu ggucuggugg ccgcagcuaa gaauauuuac      900

gauuggucuu ugcucacugg cguccugggg gaaugcaaau ccaucucaga ggccaaggug      960

aaaaccuaug agaagcacag cgaagacuua gcggcccuga ggcggguggu ccgaaaauac     1020

cauccagaca aguauaagga gauuuucaaa aaguacguug ugggaaacua uacaaguuac     1080

ucuggcaaug uaaaauccgc agguaagauc agcgaaacua aacucaagcg cuguaaccaa     1140

gcugaguuuu gcgauuaccu uaagucgaaa cugaaggacg ugaaaccggc ccccggggau     1200

gacgaaugug agagagucuu gaaggagauc ucuaaacaca ccuucugccc uagacaggug     1260

acaaccgaua accggguuau uccauaucag cuauacuaua cggaacugaa gaaggugcuc     1320

gagaaugcua aacgcuacau accccagcug aacgucacug acgaauaugg auccgccgca     1380

gauaagaucc uuucacugau gacauuuagg auuccuuacu augugggccc auugguuaaa     1440

aagaaugaca guagcaacgc cuggaucgua aggcguggcg aggggcggau acuccccugg     1500

aauuucgaug agcuggugga caaagauaag ugugaagacg aguuuauucg aagaaugacc     1560

ugcaagugua cuuacaucgc ugggaaaaac gucuuaccga aaaauucccu gcuauacugc     1620

aaauucaugg ugcucaacga aaucaauaac auuaagauca auggugagag gauuucuguc     1680

ggcgauaaac aaaagauaua uacagaacug uuugagaaaa agcgcaaggu gaccguucgg     1740

gacaucagaa accuuuugac gagcgagggg aggcugaaag cgggagacac cuucggcggu     1800

auugauauca cagugaaguc aagucucaaa ccucagcaug accugaagcg ccuuauggca     1860

gccgggguaa ucacugaagc ugaugccgag gaaauuauag agcgaauuac cugcacaacu     1920

gacgucuccc ggcugcguac cugguugggc aaauuuggac ucagcgcagc cgaugcuaag     1980

uacgugucug gccugaagua uucagacuuc gggagauuaa guagggaguu ccugacagaa     2040

guuaaugaug uggaccccgc caccggagag gucaucaacc caaauaucau uacuaugaug     2100

ugggaaaaca acaucaaucu aauggagcuc cugagccaga aauacuccua ucgcucucaa     2160

auagaaucga ugaaccggga guacuaugca gcgaauccuc uuacguugga ugaaagaaug     2220

agcgagaugu acauccccuc cgcugugagg cgaccaguaa agcgcacaau ugagaugaug     2280

aaagaauaca ugcacaucau gcggaagccc ccuaaaagaa uuuuuaucga gauggccagg     2340

gaacugaccg accucgauaa caaggguaag cguacuucaa gucgccggaa uacccuguua     2400

gaguucuuua aaaagugucc acaggacaca accgagcuga gagcacagcu ugagaaaaag     2460

ucugaugacg aauugaggag cgaaaaacuc uaucuguacu ucgcccagcu aggcaagugc     2520

auguauuccg gggagucaau agacauugaa cgacugggaa gcaagcucua cgauguggac     2580

cacaucuuuc cgcaagcucg gguuaaagau gacucuauug agaacaaggu ccuggugcuu     2640

uccgauuuga auggcgccaa aagugaccgc uaucccauca acgcagauau aagaaauaag     2700

augcauagcu ucugggacaa acugcacucu aucgguuuaa uuggggagaa gaaauacgaa     2760

aggcucacuc gguccacagg cuuuacggau gacgagcugg cuggauucau cucacgacag     2820

cuuguggaaa ccagacagag uacuaaggcc gucgcggccc uguuggcuga ggaauuuccu     2880

gagagcgaga uuguuuaugu gaaggcaggc cucgccucgc aguuccgcca ugaauuuggg     2940

cugcuaaaau guagggagau caacgaucug caccaugcua aggacgccua ccucaauaua     3000

gugaugggaa acauuuacca cguaaaauuc acaaaggacc caaugaauuu ugucaaagaa     3060

uaucgucgga aggagcaagu guucgaucug aagauccagu ccaugcuuac ccacgacgca     3120

aucagaaacg gugaaacagc guggauuggc gaugggacuu gguuggagac cguuaaaaag     3180

cagauggaga aaaauaacau cagguacgug cgcuauucuu uccgaagaaa gggagcccug     3240

uuuaacuuaa ugcccgaacg gaaaaauccu agccuccugc caaggaagaa cggcaagaau     3300

augguccuuc ccgcugagaa auacgguggg uauaacaaua cggccgcauc auucuuuagu     3360

cuggugaagu accauuccgc uaaaggcucu ggaguuguga uuauaccggu agaacuaauc     3420

cacgcccauc gcuaugagca cgacagcucc uucgcagagg aauacgcccu ccguguccug     3480

gcugauauug ugucauugaa gaaaggcgac gugcugcaga gcgucgaguu uccuuuaggg     3540

aguagaaucc ucaagguuaa cacccugcuu gauauugacg gauuccgggc gaauaucaca     3600

ggcaaggauu cuggugggaa auaccuggga auauccagcg ccucgucauu gaucauuucu     3660

caaguggaca cuaccuauau caagagggua auuaguauca acgaacgcau auccgcaugc     3720

gagaaaaaua agcagagcua cacaauucga ccagaauuug augugaucac uucugagaga     3780

aacaucgaga uuuauaauau gcucugugag aaaauaagcu cccggcccuu cugcaucaag     3840

uuugcuaaca ccucagacac guucgucagu ggcagggaaa aguuuauugc ccugagccua     3900

aaugaacagg ugaaaacacu gaccaacaug cuuucuguuu ucaagacugg ucgcgccucc     3960

ggcugugauc ucacaaauau cgggggaagu aaaaacaccg cuauugugaa gcugaacgca     4020

gucuugucaa aucugaaaga cuucaagagc gugagaauca uagaccaguc cccuacuggc     4080

uuauacgaga aaguuucucc caaccuccug ucgcuuuga                            4119


<210>  3157
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3157
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucauaacug augacuauaa gauccccgcu aaaaagauga aaguucucgg uaacacggau      120

aaggaguaca uuaagaaaaa ucuucugggc gcauuguuau ucgacucugg ggaaaccgcc      180

gaggcgacaa gacugaagag gacugcucgg cgccgauaua cccguagaaa gaacaggcua      240

cgguaccucc aggaaaucuu ugccgaggaa auugcaaagg ccgauaauuc auucuuucaa      300

cgccuggacg agaguuuccu uacagaugac gauaaagacu uugauagcca cccuaucuuc      360

ggaaacaagg cugaggaaga cgcauaucau cagaaguuuu ccaccauaua ccaccugaga      420

aaauauuugg ccgauucucc agagaagucg gaccucaggc ugguguaccu ggcucucgcc      480

cauaugauca aauaccgggg ccacuucuua auugaaggug agcugaaggc ggagaauacu      540

gacguacaga aacuuuuuac agauuucgug gaagucuaug accgcaccuu ugaugacuca      600

cauuugagcg aggugacggu ugaugcaagu uccauccuga cugaaaagau uucuaaaagc      660

cgaagacuag agaaccucau cgaauccuac ccgaaggaga agaaaaauac ccuguucggg      720

aaccuuauag cccugucauu gggacuccag cccaauuuua agacaaacuu caaacugagu      780

gaggacgcua aguuacaauu cagcaaagau acuuaugaag aggaccugga agaggugcuc      840

gaaaagauug gcgaugacua cgccgaucuu uuuaccucug caaagaaucu guaugacgcu      900

aucuugcuau ccggcauucu gacagucgac gauucaagca ccaaagcccc ucucagugcg      960

ucuaugauca agagguacgc cgagcaccac gaggaccugg aaaaacuuaa ggcauucauc     1020

aaaacgaaca agccaaaucu guaucaugau auuuuuaagg acgagacuca gaacggguac     1080

gcuggauaca uagaaaacgg ugugaaacag gaugaguucu auaaguacuu gaaaacaauc     1140

cucuccaaga ccgacggcag cgaauauuuu cuggauaaaa uugagcggga ggacuucuua     1200

cguaagcagc gcacuuuuga uaaugggucg aucccccacc aaauacaucu gcaggaaaug     1260

cacgccauuc uaagaaggca gggagacuac uauccuuucc ucaaggagaa ccaggaucgg     1320

aucgagaaaa uccugacauu ucgaauccca uacuauguag gcccccuugc ucgcaaaaau     1380

ucuagauucg ccugggcaga auaccacucc gacgagaaaa uuaccccuug gaacuuuaau     1440

aagguuauug auaaagaaaa gucagccgag aaauucauaa cgaggaugac cuugaacgac     1500

cuguaccucc cagaagagaa ggugcugccg aagcauaguc acgucuauga aacauuugcu     1560

guguacaaug agcuuacuaa aaucaaguau gugaacgagc aagguaaaga agcguucuuc     1620

gacagcaaua ugaagcagga gauuuuugau caugucuuca aagaaaaccg gaagguuacc     1680

aaggagaaac uguugaauua ccucgacaag gaauuucccg aguaucgcau ccaggaucug     1740

aucggguuag acaaagagaa caaguccuuc aaugcaucuc ugggaacaua ccacgaucua     1800

aagaagauuc ucgacaagag cuuucuggau gacaaaguga acgaagaggu aauagaagau     1860

aucauuaaga cucuuaccuu guucgaggac agagaaauga uccaacagag gcugcauaaa     1920

uauucagaua uuuuuaguac acagcagcuc aagaaacugg agcgucggca cuacaccggc     1980

uggggccgau uauccuacaa gcugaucaau gggauaagaa acaaggagaa caauaaaacu     2040

auccuugaau auuugauuga cgauggauac gccaacagga auuucaugca acucaucaac     2100

gacgacacgc ugccuuuuaa gcagauuauc aaagaugcuc agauaauugg uaauaucgac     2160

gauauugccg caguggucca cgagcuacca ggcagccccg ccaucaagaa agggauacug     2220

cagucuauca agauugugga cgaacucguu aaagugaugg gacauaaccc ugauaauauc     2280

gucauugaga uggcucgcga gaaccaaaca accaaucggg gcagaucaca gagucagcag     2340

aggcugaaga aacuucaaga cagcuugaaa gaacuggguu ccaacaucuu aaauaagaaa     2400

aagccaucuu auguggagga uaaaguagaa aacguucacc uccaggacga ucgccuguuc     2460

cuuuacuaua uacagaaugg gaaggacauu uacacuggcg aggaacugga uaucgaccga     2520

uugucggacu augauaucga ccauauuauc ccccaggccu uuauuaagga ugacagcaua     2580

gauaaccggg ugcucaccuc cucagcgaaa aaucguggaa agagugacga ugucccgaac     2640

cuggacaucg ugagagcaag gaaagcugau uggguccgcc uauacaaguc uggccugauu     2700

agcaaacgga aguucgacaa ccucacaaag uccgagagag ggggacugac cgaggccgau     2760

aaagcagguu uuaucaagag gcaacuugug gaaacucgac agauuacaaa acacguugcc     2820

cagaucuugg acgcucgcuu caauacggag cacgaugaag acgacaaggu gauacggaac     2880

guaaaaguca ucacccugaa gucaaauuua auuagccaau ucagaaagga uuuuaaauuc     2940

uacaagguga gggagaucaa cgacuaucau cacgcccaug augcauaccu caaugcuguu     3000

gugggcacug cccugcuucg uaaauauccu aagcuggcgc ccgaauuugu cuacggggag     3060

uauaaaaagu acgacgugcg caagcuaauu acaucuucca gugauaaagc caccgcuaag     3120

uauuucuuuu acagcaaccu caugaauuuc uuuaaacggg uagugagaua cucuaacgga     3180

aggguuaucg uccgaccagu gauagaguau uccaaggaca caggcgaaau uguguggaau     3240

aaagagacug auuuccggac caucugcaag guccugucau acccucaggu uaacaucgug     3300

aaaaaggugg aaagucagac ggguggguuu agcaaggagu cgauuuugcc caaaggcgac     3360

uccgauaagc ugauaccacg caaaaccaag aaaguauauu gggacacaaa gaaguacgga     3420

ggcuucgauu cucccaucau ugcauauagc gucuuugugg uugccgacgu ggaaaaaggg     3480

aaggcuaaaa agcucaaaaa ggucaaggag uuagugggaa ucucaauuau ggagagaccu     3540

aguuucgaga aaaauccaau cguuuuccug gaaaagaaag guuacaggaa cgugcaggaa     3600

gauacucuua uaaagcugcc gaaauauucc uuguuugagu ucgagggcgg gcggcgaaga     3660

cuccuggccu cugcaagcga acuacaaaag ggaaaugagg uaguccuguc cgaccgccuu     3720

guggaacucc uguaccacgc gcauagggug aacucauuua auagcaccga gcacuugaag     3780

uacgucagug aacacaaaaa ggaguucggc gagguucugu cuugugugga aaacuuugcc     3840

aacuccuaug uagacgugga gaaaaauuua gauaagaucc gugcugucau uaacaaaauc     3900

gacacauuca gcauugaaga gaucucgacu ucauuuguga aucuccugac ccuuacggcc     3960

cugggugcac ccgcugauuu caacuuuuug gggaagaaaa uaccucggaa gagauacaca     4020

ucuaccaagg aaugccucaa ugccacucug auucaucaga guaucacagg ccuauaugag     4080

accaggaucg accuguccaa acuuggagag gauaaguga                            4119


<210>  3158
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3158
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucauaacug augacuauaa gguucccgcu aaaaagauga aagugcucgg uaacacggau      120

aagaaguaca ucaaaaagaa ucuucugggc gcauuguuau ucgacucugg ggagaccgcc      180

gaagcgacaa gacugaaaag gacugcucgg cgccgauaua cccguagaaa gaacaggcua      240

cgguaccucc aggagauuuu ugccaaugaa aucgcaaaag uagaugaguc auucuuucaa      300

cgccuggacg aaaguuuccu uacagaugac gauaagaccu uugacagcca cccuauuuuc      360

ggaaacaagg ccgaggagga ugcuuaucau cagaaauuuc caacuaucua ccaccugaga      420

aagcauuugg cagacuccuc ugaaaaagcc gaucucaggc ugguguaucu ggcucucgcc      480

cacaugauaa aguuccgggg ccacuuuuua aucgagggug aacugaaugc ggagaacaca      540

gacguccaga aaauuuucgc agacuuugug gggguuuaca aucgcaccuu cgaugacucg      600

caccuuucag agaucacggu ggaugucgcc agcauuuuga cugaaaagau caguaaaucc      660

cgaagacugg agaaccuaau aaaguacuau ccgaccgaaa agaaaaauac acucuuugga      720

aaccugauug cucuugcccu gggcuugcag cccaauuuca agacuaacuu caaacucucu      780

gaggacgcaa agcugcaauu uagcaaagau accuacgaag aggacuuaga ggaacugcuc      840

ggcaagaucg gggaugacua ugcugaucuu uucacauccg ccaagaaucu guacgacgcg      900

auuuugcuau caggaauccu gaccguggau gacaacagua cgaaagcccc ucucagcgca      960

ucuaugauca agagguaugu agagcaccau gaagaccugg agaaacuuaa ggaauuuauu     1020

aaagcuaaca aguccgagcu guaccacgau auauucaagg acaaaaauaa gaacgguuau     1080

gccggcuaca ucgagaaugg gguuaaacag gaugaauuuu acaaguauuu gaaaaacauu     1140

cucucaaaga ucaagauaga cggaagcgau uacuuccugg acaaaauuga gcgggaagac     1200

uuuuuacgua agcagcgcac uuucgauaau ggcaguaucc cacaucagau ccaucugcaa     1260

gagaugcacg cuauucuaag aaggcagggu gauuauuacc ccuuucucaa agaaaagcag     1320

gaccggaucg agaaaauucu gacauuccga auaccuuauu acguggggcc acuuguccgc     1380

aaggauucua gauuugccug ggcagaguau agguccgacg aaaagaucac ccccuggaac     1440

uucgauaaag ugauugacaa ggagaaaagc gccgaaaagu uuaucacucg gaugacauug     1500

aaugaccugu accucccuga ggagaaagug cugccaaagc auucgcacgu cuacgaaacc     1560

uaugcuguuu acaacgagcu uacgaaaauc aaguauguga augaacaggg aaaggagucu     1620

uucuucgauu ccaacaugaa acaagaaauu uuugaccaug uauucaagga gaaucgcaaa     1680

gugaccaagg agaaacuguu gaacuaccuc aauaaggaau uuccggagua uagaauaaag     1740

gaucugaucg gcuuagacaa agaaaacaag ucauucaaug cgagucuggg cacauaccac     1800

gaucuuaaaa agauucucga caaagcauuu cuggaugaca aggucaacga ggaagugauc     1860

gaggauauua ucaagacucu uaccuuguuc gaggacaaag auaugauaca ugaaaggcug     1920

cagaaguaua gcgacaucuu uacagccaac cagcucaaaa agcuggagcg ucggcacuac     1980

acuggguggg gacgauuauc cuacaaacug auuaauggua ucagaaacaa ggaaaauaac     2040

aaaaccauuc uugauuauuu gaucgacgac ggcucugcua auaggaacuu caugcaacuc     2100

auaaaugaug acacacugcc cuuuaagcag auuauccaaa agagccaggu ugugggggau     2160

gucgacgaua uugaggccgu gguacacgac cuaccuggau caccagcaau caaaaagggc     2220

auacugcaga guguuaaaau cguggaugaa cucgucaagg ugaugggugg gaaccccgac     2280

aauauuguca ucgagauggc ccgcgagaac cagaccacua aucggggcag aucccagagc     2340

cagcaaaggc ugaaaaagcu acagaacucu uugaaggaac ugggaucaaa uauuuuaaac     2400

gaggaaaaac cuaguuacau cgaggauaag guggagaaua gccaucucca gaacgaccag     2460

cuguuccuuu auuacauaca aaacggcaaa gauauguaua cgggggacga acuggacauu     2520

gaucacuugu ccgacuacga uaucgaccau aucauuccac aggcuuuuau caaggaugac     2580

ucuauugaua aucgcguucu cacaucgagc gccaaaaacc gaggaaaguc cgacgaugug     2640

cccucacugg acauaguacg ggcacguaag gcggaguggg ucagacuaua uaaaaguggu     2700

cugaucucua agaggaaauu cgauaaucuc accaaggcug aacgcggcgg gcugacugag     2760

gccgacaaag caggauuuau uaagcggcag cuuguggaaa ccagacagau cacaaagcac     2820

guugcccaaa uuuuggaugc uagguucaac accgagcacg acgagaauga caaagugauc     2880

cgagauguca aggugauaac ucugaaaagc aacuuaguau cccaguuccg caaggacuuu     2940

gaguucuaca aagugcggga aaucaaugau uaccaucacg cccaugacgc auaucucaac     3000

gcuguugucg gcacagcccu gcuuaagaag uacccgaaac uggcgucaga auuuguguau     3060

ggugaguaca agaaauauga uauuagaaag uucaucacga auagcucuga caaagccacc     3120

gcuaaguacu uuuucuauuc caaccuaaug aacuuuuuca aaacuaaggu gaaguacgca     3180

gaugggacag ucuuugaaag gccuauuauc gagaccaaug ccgacggcga gauagcuugg     3240

aauaaacaga uugauuucga aaagguucgu aaagugcuca guuaccccca agugaacauc     3300

guaaagaaag ucgagacaca gacuggaggc uuuagcaagg aaucuauccu gccaaagggg     3360

gacuccgaua aauugauucc ucgcaagacc aaaaaggugu auugggacac gaaaaaguac     3420

ggagguuucg auucacccac cguugccuau aguguguucg ucguggcaga cguugagaag     3480

ggcaaagcga agaaacugaa gacagugaaa gaauuaguag ggauaagcau cauggagcgg     3540

ucguuuuucg aggaaaaucc agucgaguuu cucgaaaaca agggauacca caacauuaga     3600

gaggacaagc ugaucaaacu ucccaaguau ucccuguucg aguuugaagg cgguaggcga     3660

cgguugcucg ccucugcuag cgagcugcag aaagggaaug aaauggugcu accuggcuac     3720

cugguggagc uucucuauca ugcccaccgc gcagauaacu ucaauucaac ugaauaccug     3780

aacuacguca gugagcacaa gaaagaguuu gaaaagguuu uguccugcgu ggaggacuuc     3840

gcuaaucugu auguagaugu ggagaaaaac uuaucuaaga uuagagccgu cgcagacagc     3900

auggauaauu uuuccaucga agaaauauca aacagcuuca ucaaucuccu gacccuuaca     3960

gcccugggag cuccagcgga cuuuaacuuc uugggcgaga agauuccgag gaaacgguac     4020

accaguacua aggagugucu caaugccacg cugauccauc agucuauuac agggcuauau     4080

gaaacccgaa ucgaucuguc caaacuugga gaggaauga                            4119


<210>  3159
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3159
augaacagcg acaagagaga ggauuacuau gugggccugg acauggggac cggaucccuc       60

gguggcgccg ucacagauac ucaguaccac cuucugaaag uuaaggggaa agacuucugg      120

uuugugaggg aauaugagac ggcuaagucu caauuggauc ggcgcaccca ucgaaucgca      180

aaacguagau uacagaggca caaggcccgg auuggacuga uacgcucaua cuucgcggaa      240

gacauccuaa agcaugaucc ccucuuuuau auuagacagg acaauaguaa auacuaucac      300

gaggauaagg acguaaggcu gacaacuaaa gauagcaucu ucgcugaccc uggcuacggu      360

gauaaggaau accuugacga caaagaguau ccaaccauuu uucaucugcg gcaggccuug      420

cuccgcgauc aaaucaagga cgaggaacga uacuccagau uccuguaucu ggcaauaauc      480

aacauguuug agcacagggg gcacuuccuc uuaaauacag aaucuucgga ucugaacgcc      540

gacaugauua aggauguggg agagcuuguc augaaugcua ucugcggcgu gucagaguac      600

ggcagcguua ccuauaaaga aauuaucgag auauuggaag acaagaacau uagucggacu      660

gccaaaaagg aucagaucgc auccaaaauu gacaucaaga aaucugagaa ggcucagaug      720

gaacguauca agugucugug cgggauggag gccgaugcgc gcgugauguu ugagauugaa      780

gcagacgaga aaauaaagau cgccuuccag caagcugccu uugaagauga gaaagaagag      840

auugaggcag ucaucggaga agacagauac cuaauaauug agaauaugaa gcagcucuau      900

gacuacagcc agcugcaguc cgugcuuaac gguuucgauu accugucaga cgcuagggua      960

caaauguaug aaaaacauaa ggaggauuug caccuccuga agaaaguuua ccggaagaau     1020

uuagaccgag aaaucuauga ucgcauguuu agaaguggcg aggaggggag cuacugugcc     1080

uauucuaacu ccacaaauuc aguggacagc cuggcggaag ccaaaaagua caggcggaac     1140

augaaaaagc gcagucagaa ggaucucuau gacagaauca aaaaggaucu uaaagagguc     1200

caugacgcag augcugacgc cauuaggaag gaaauggagc uggagcguuu cuugccgaaa     1260

cagaucaccg gagcuaaugg cauuauaccc aaccaagugc accggaagga acuaaagaaa     1320

auccuggaga augccgaaca ccaucucgau uuccugaagg agaaagacga aucugguuac     1380

acgguguccg agcgaauuuu gaugcuguuu agcucgaaca ucccuuacua ugucgggcca     1440

guuggaucug gcuccaagac uggcugggca aaaauccagg agucaggaca gguguugccc     1500

uggaacaugg aacagaagau ugacauagag caaaccagug aaagauucau cacaaaucuc     1560

auuagggagu gcacuuaccu gagcggggaa aaaguauuac cuaagcaguc ccugcuauau     1620

gagucuuacu gugugcucaa cgagaucaau aaccugcgca uucacgguaa gcggaucguc     1680

ccagaacuua aacaggauau auauaaggag guguuucagg gcuucagauu ggggaaaaag     1740

guuaccaaaa agaauaucug cacauaccug auuaacaggg gacucaucac cgaagagagc     1800

gaagugucag gcauugacaa ggagaucaau gccuaucugc ccaguuacgg uaaaauguac     1860

ggggucuuug gcgagcgccu ucgagaagag gcugugcaag aaauagcgga gaagauuauc     1920

uauuacggaa cgauuuaugg cgaugcaaaa uccauguucc ggaagaaacu gaaccaguac     1980

guaagcgacg gguggaugac ugaguaucag gccaagcgua ucucuggaua caaguuuaaa     2040

gauugggcua gauugucaag gaguaugcuc ugucugagcg guugcgacaa guccacaggc     2100

gagaaaauau cuuuaauccg cgccaugugg gaauauaauc ugaacuucau ggaacuaauu     2160

uaccgggagg auuuuaccuu ccagaaggaa cucgaggaga gaaggaaagc acuggggaag     2220

acucuucaag aauuucaguu cgaggacuug gaugaauacu auuacucggc cccuguuaag     2280

cgaaugaucu ggcaggcugu gcaggcccug aaagagauua cacaaaucau gggacauaau     2340

ccaaagcgca uauuuauuga aaugacccgg acggaggagg aaaaaggcga gcaggguaga     2400

aagaacagca gggaaaaacg ucuccugaag auguaugagu ccauccgcga cgaacggaau     2460

ugggcagcgg agaucaaaaa cgcuaaugcc ucagggaagu uaaacaguaa gaaacuguac     2520

cuuuauuacu ugcagauggg cagagauguc uauaccggag agccgauuga caucgaagau     2580

cucuucgacg acucuaggua cgauauugac cacauauacc cccgaagccu gacaaacgau     2640

aauaacaucg agaauaaccu agugcugguc uccaagacua uuaaucaaaa cgaaaagaaa     2700

aaugacuauc cugugccaga gaaaauccgg cgcaacaaga agguuagaaa ucucuggcau     2760

acccugcaca aaaugggcuu caugaacgau gagaaguaca auaggcuuac aucaacugaa     2820

cgguugagcg aggaccagcu ggcaggguuu auugcccgac aguuagugga aaccgcucag     2880

ggagccaaag guaucgcaga gcucaugaag gcuaugcugc ccggcacaga uauaguauau     2940

gccaaagcga gaaacgucuc ugacuuccgc aaggaauaca agaugcuuaa auccaggcug     3000

gugaaugauu aucaccaugc ccaagacgcu uacuugaaca ucguuguggg gaaugucuau     3060

uuuaugaagu ucacccguaa cccuaugaac uuugugaaaa cugaggcacg gagagaugga     3120

aggaauuacg acuacaaccu cuauaagaug uacaguaaag acauuauccg caauggcgag     3180

aaggccugga uugcuacgag cgaacagggu ccagggacaa uccgacuggu aaaggagacc     3240

augggcagaa acacucccau aauuacccgg caggccuucg aacagagggg agagcuauuu     3300

aaucugcaac cggugggcaa auauucugca aagaaagaua acuacguucc ucucaagauc     3360

aaugacgaga aaaugcagga ugucuccaag uaugggggau acacaucacu gaaccccagu     3420

uauuucaucu uuauugaaca cggcccagag aagaaacgca agaaauguuu cgaagugaua     3480

cauagcuacu acgcggccca gaucaagacc gagaaagacc uuauugauuu ucuucugcag     3540

aaggguuaua aaaauccucg ugugaucaac gcuagaauua agaagaaugc cuuaaucaaa     3600

uacaacgggu auuuccucua cauaaucgga auggacgcac ggaagaauau ugaauuuucg     3660

aacgcuacug ccaugugccu gaaaaauaag uauauccaau acgucuguaa acuugagaag     3720

augaacaagg caauucugcu auccgaaaaa cagaagacaa accuccacug ggaugagaaa     3780

aucacgugcg agucuaaucu ggaauuguau agggagcuga ccgaaaagca cuuacauagc     3840

auauaccagc gccacccccg aucaauuggc aaaugccucg ccgacgguga gggcgcuuuc     3900

aagcugcuug auaucgagga acaaguuaag aucauuugug acaucgugca guacacaagu     3960

uuccagagag ggguguuuuc ccugaaagua uugggaggcc caaaggaggu cgggcggaua     4020

aggauuucug gaaacaugac ugaagcgaaa gagugcaagc ucgugaauua uagcaucacc     4080

ggcauguaca aaacagaaau ggaucugcua aagaaguga                            4119


<210>  3160
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3160
auggagaaga ccuacagcau cggccuggac auugggacaa acuccguggg auggucuguc       60

auaacugaug acuauaaagu ucccgccaag aaaaugaagg ugcucgguaa uacggauaaa      120

aaguacauca agaaaaaccu ucugggcgcu uuguuauucg acucagggga aaccgcagag      180

guaacaagac ugaagaggac ugcccggcgc cgauauaccc guagaaagaa uaggcuacgg      240

uaccuccagg aaauuuuugc gaaggagaug gcuaaagugg augaaaguuu cuuucaacgc      300

cuggaggaga gcuuccuuac agacgaugac aagaccuuug auucccaccc uaucuucgga      360

aacaaggccg aagaggacgc auaucaucag gaauuuccaa cuauuuacca ccugagaaaa      420

cauuuggccg auucuucgga gaaggcugac cucaggcugg ucuaucuggc acucgcccac      480

augaucaaau uccggggcca cuuuuuaaua gagggugaac ugaaugcuga gaacacagau      540

auccagaagu uguucaauga cuuuguggaa accuacgaca aaauugccga ugagucacau      600

uugagcgaag uugacgcgag uuccauccug acgaacaaga uuucuaaaag ccgccgacua      660

gagaaucuca ucaagcagua cccgacugag aagaaaaaca cccuguucgg gaaucuugug      720

gcacuggucu ugggacucca acccaacuuu aagacaaauu ucaaacuguc cgaagaugcc      780

aaguuacagu ucucaaaaga cacuuaugau gaggaccugg aggaacuccu uggcaagaua      840

ggcgaugacu acgcugaucu guuuaccgcc gcaaagaacu uguaugacgc uauucuacug      900

agugggaucc ucacagugga ugacaauagc accaaagccc cucugucugc guccaugauu      960

aagagauacg aggaacacca ugaggaccuu gaaaaacuga agaaauucau caagguaaac     1020

aacuuugaua aguaucacga gaucuucaaa gacaagucaa agaauggaua cgccgguuau     1080

auugaaaacg gcguuaagca ggauauauuu uacaaacauc uuaagagcau cauuagugag     1140

aagaaugggg gacaguacuu ccucgacaaa aucgaaaggg aggauuuucu gcggaagcaa     1200

cguacguucg acaacggcuc uauaccacac cagauucacu uacaggagau gcgcgcaauc     1260

cugagaaggc agggugaaua uuaccccuuu cuaaaagaga aucaagcuaa gaucgaaaag     1320

auucucacuu uccggauccc uuauuacgug gggccacugg cccgaaagga uucccgcuuu     1380

gcuugggcca acuaucauag cgacgagccc auuacaccuu ggaauuucga ugaggucgug     1440

gacaaagaaa agucggcaga gaaguuuaua accagaauga cucuuaacga uuuguaccug     1500

ccagaagaga aagugcuccc gaagcacucu caugucuaug aaacauucac cguuuacaau     1560

gagcugacga aaaucaagua cgugaacgag cagggagaau ccuucuuuuu cgacgccaau     1620

augaaacagg agauuuuuga ucacguauuc aaggaaaaca ggaaggugac caaagcuaaa     1680

cuucugucau auuugaauaa cgaguuugaa gaguuccgga ucaaugaccu caucggccug     1740

gacaaagaua guaagagcuu uaacgcgucc uuaggcacau accaugaccu gaaaaagauu     1800

cuagauaagu cuuuccucga cgauaaaacu aacgagcaaa uaaucgaaga cauuguccug     1860

acccuuacau uguuugagga ucgcgacaug auccacgaaa gacugcagaa guauagcgau     1920

uucuuuacuu cacagcagcu caaaaagcug gagaggcguc acuacaccgg guggggacgg     1980

uuaaguuaua aacugauuaa ugguauccga aacaaggaaa auaacaagac aauacuugac     2040

uucuugaucg augacggcca ugcaaauaga aacuuuaugc aacucauuaa ugaugagucc     2100

cugagcuuca aaaccaucau ucaggaggcc caggugguug gggacgugga cgauaucgaa     2160

gcugucgugc acgaccuacc cggaucuccu gccauaaaga aaggcauucu gcagucagua     2220

aagaucguug augagcucgu gaaagucaug ggugacaacc cagauaauau ugugaucgaa     2280

auggcaaggg agaaccaaac uacgaaucgc gagcggaguc agagccagca gagacugaag     2340

aaacuucaaa acuccuugaa ggaacugggg ucuaauauau uaaacgagga aaagcccucg     2400

uacaucgagg acaaagucga aaauagccau cuccagaacg aucagcuguu ccuuuauuac     2460

auucagaacg gcaaggacau guacacagga gaugagcugg acaucgauca cuuguccgac     2520

uaugacauug aucacaucau accucaagcc uuuauuaaag acaauucaau cgauaacagg     2580

gugcucacca guucugcuaa gaaucgcggc aaaagcgacg auguuccauc ccuggacauc     2640

gugcgagccc ggaaggcaga guggguacgu cuauacaagu cagggcugau uagcaaaaga     2700

aaguucgaua accucacuaa agcggaaagg ggaggucuga ccgaggcuga caaggccggc     2760

uuuaucaaac gccagcuugu cgaaacacgg cagauuacca agcauguggc acaaauauug     2820

gaugccagau ucaauacuaa gagggacgag aacgauaaag uuauccgaga cgugaagguc     2880

auuacacuga aaucuaauuu agugucccag uuucgcaagg aguucaaauu uuauaaggua     2940

cgggaaauca acgauuacca ccaugcucac gacgccuauc ucaaugcagu gguugggacg     3000

gcucugcuua agaaauaccc caagcugacc ccggaguucg ucuauggaga auacaaaaag     3060

uacgacguga gaaaacuaau ugccaagagu agcgaugacu auucugagau gggcaaggcg     3120

acugccaaau acuuuuucua uuccaaccuc augaacuuuu ucaagacaga agugaaauac     3180

gcugauggua gggucuucga gcguccugac aucgaaacca augcagaugg ggagguugug     3240

uggaauaagc agaaagacuu ugauauagug cgcaagguac ugucauaucc ccaggucaac     3300

aucgugaaaa agguugagga acaaacagug ggccagaaug gaggcuuguu cgacaacaac     3360

auucuggcca agccagagaa agaugacaag aaaaaguuau uuccuaucaa aaagagucuc     3420

agcacuaccu cguacggggg auaugcucgg gccacgauug cauacuccau ccugauaguc     3480

ucugaguucg guaagaauaa agaacuuaag cuguugggcc ucccccugcu agauaaagug     3540

aaguacgaga aaaacccaau ugcguaucug aauagcaucg ggaucauuaa cguuaaguca     3600

auauuuaaac uucccaaaua cugccucuuc gaauuucagg agaagaauac caacuauaga     3660

agguacaugg ugaguaauca ggaacugaaa cgagccaacc aauuguuccu gacauguaag     3720

gagacugaau uaguauauaa auacaucaag uccgacaaug auaacaccca ucucgucgga     3780

aaugagaaga caauucuguc uaucuggaac aaacuuguga uuuuuaugga gcggacuaag     3840

cugaucgaca aaaaguuguu cgcuagccuc aaacagcuga aggacgaagu ggaucuaaua     3900

aauauccugg acaugaccgc caaaucccuu gauuuuacga agauuucagc aggccagaag     3960

aaaaucacaa uuagcgacag ucucucucug ccuaucaagc gcuggcaguu cuccagcgau     4020

gagaaaaugu ugaagcaauu aaaaaccgcu acucugauac accagucggu cacaggucuc     4080

uaugaaacca gaauugaccu gucaaaacuu ggggaggaau ga                        4122


<210>  3161
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3161
auggagaaga ccuacagcau cggccuggac auugggacaa acuccguggg auggucuguc       60

auaacugaug acuauaaagu ucccgccaag aaaaugaagg ugcucgguaa uacggauaaa      120

aaguacauca agaaaaaccu ucugggcgcu uuguuauucg acucagggga aaccgcagag      180

guaacaagac ugaagaggac ugcccggcgc cgauauaccc guagaaagaa uaggcuacgg      240

uaccuccagg aaauuuuugc gaaggagaug gcuaaagugg augaaaguuu cuuucaacgc      300

cuggaggaga gcuuccuuac agacgaugac aagaccuuug auucccaccc uaucuucgga      360

aacaaggccg aagaggacgc auaucaucag gaauuuccaa cuauuuacca ccugagaaaa      420

cauuuggccg auucuucgga gaaggcugac cucaggcugg ucuaucuggc acucgcccac      480

augaucaaau uccggggcca cuuuuuaaua gagggugaac ugaaugcuga gaacacagau      540

auccagaaac uuuucaauga cuuuguggaa accuacgaca aaauugccga ugagucacac      600

uugagcgaag uugacgcgag uuccauccug acgaacaaga uuucuaaaag ccgccgacua      660

gagaaucuca ucaagcagua cccgacugag aagaaaaaca cccuguucgg gaaucuugug      720

gcacuggucu ugggacucca acccaacuuu aagacaaauu ucaaacuguc cgaagaugcc      780

aaguuacagu ucucaaaaga cacuuaugau gaggaccugg aggaacuccu uggcaagaua      840

ggcgaugacu acgcugaucu guuuaccgcc gcaaagaacu uguaugacgc uauucuacug      900

agugggaucc ucacagugga ugacaauagc accaaagccc cucugucugc guccaugauu      960

aagagauacg aggaacacca ugaggaccuu gaaaaacuga agaaauucau caagguaaac     1020

aacuuugaua aguaucacga gaucuucaaa gacaagagca aaaauggaua cgccgguuau     1080

auugaaaacg gcguuaagca ggauauauuu uacaaacauc ucaagagcau cauuagugag     1140

aagaaugggg gacaguacuu ccucgacaaa aucgaaaggg aggauuuucu gcggaagcaa     1200

cguacguucg acaacggcuc uauaccacac cagauucacu uacaggagau gcgcgcaauc     1260

cugagaaggc agggugaaua uuaccccuuu cuaaaagaga aucaauccaa gaucgaaaag     1320

auucucacuu uccggauccc uuauuacgug gggccacugg cucgaaagaa cagccgcuuu     1380

gccugggcua aauaucauuc ggaugagccc auuacaccuu ggaauuucga cgaggucgug     1440

gauaaggaaa agucugccga gaaauuuaua accagaauga cucuuaacga cuuguaccug     1500

ccagaagaga aggugcuccc gaaacacucc caugucuaug aaacauacgc aguuuacaau     1560

gagcugacca agaucaaaua ugugaacgag cagggaaagg aaucauucuu cgauaguaau     1620

augaagcagg agauuuuuga ccacguauuc aaagaaaaca ggaaggugac gaaagagaaa     1680

cuucugaauu acuugaacaa agaauuuccc gaguaucgga ucaaggaucu caucggccug     1740

gacaaggaga auaaaucauu caacgccucc uuaggcaccu accaugaccu gaagaaaauu     1800

cuagauaagu cuuuucucga cgauaaaguc aacgaagagg ugauagaaga caucauuaag     1860

acacugacuc uuuucgagga uaaggacaug auccacgaac gcuugcaaaa auauagcgau     1920

auuuuuaccg cuaaucagcu gaagaaacuc gagagaaggc acuacacagg guggggacgu     1980

cugucauaua aguuaaucaa cgguauacgg aauaaagaga acaauaagac uauccuggac     2040

uaccuuauug augacggcag ugcgaaccga aauuucaugc aguugaucaa cgaugacacc     2100

cucccuuuua aacagauuau ccaaaagucc cagguugugg gggaugucga cgacauagaa     2160

gcagugguac augaucugcc aggaagcccc gccauuaaga aaggcauccu acagucuguu     2220

aagauugugg acgagcuggu caaagugaug ggugggaauc cugauaacau cgucauagaa     2280

auggcuagag agaaucagac aaccaacagg ggccgcucac aaagucagca gcggcucaag     2340

aaacugcaga gcucccuuaa ggaguuggga ucuaauauca uuaacgaaga gaagccaucg     2400

uacaucgaag acaaagugga gaauagccau cugcaaaacg aucaguuauu ccucuauuac     2460

auucagaacg gcaaggacau guauacuggg gaugaacugg acaucgauca ucuuuccgac     2520

uacgauauag accacauuau cccccaggcc uuuaucaaag acgauucaau ugacaauaga     2580

guucugacga guucugcaaa gaacagggga aaaagcgaug acgugccguc cuuggauauc     2640

guacgcgccc gaaaggcuga guggguccgg cucuauaagu caggucugau uagcaaacgu     2700

aaguucgaca aucuaacaaa agccgagaga ggcgggcuga ccgaagcaga uaaggcggga     2760

uuuauaaaaa ggcaacucgu ggagacucgc cagaucacca agcacguugc ucagauucug     2820

gacgcccggu ucaacacaga acaugaugag aaugacaagg ugaucagaga ugucaaagug     2880

auuacccuua agucuaacuu gguaucccaa uucaggaaag acuuugaguu cuacaaggug     2940

cgagaaauca augauuauca ccaugcacac gacgccuacc ugaacgcugu ugucggcacu     3000

gccuuacuca aaaaguaccc uaagcuggca cccgaguuug uguaugguga auacaaaaag     3060

uaugacgugc gcaaacuuau agcuaagagu agcgaugacc auucugagau ggggaaagcc     3120

acagcgaagu acuucuuuua uuccaaucug augaacuucu uuaaaacgaa ggucaaguac     3180

gccgauggcu caguuuauga acggccaauc auugagacca augcugacgg agaaguggug     3240

uggaacaaac agaaggauuu cgagaucauu agaaaaguac uaaguuaccc ucaggucaau     3300

aucgugaaga aaguugagga acagacugug ggccaaaacg ggggacucuu ugacaauaac     3360

auacuggcaa agcccgagaa ggaugacaaa aagaaauugu ucccaauuaa gaaaagccug     3420

ucgacaaccu ccuacggugg cuaugccagg gcuacaaucg ccuacucuau cuuaauuguc     3480

agcgaauuug ggaagaacaa ggagcucaaa cugcuuggac ugcccuugcu cgauaaggug     3540

aaauaugaga agaauccuau agcauaccug aacucaaucg gcauuaucaa uguuaaaagu     3600

auuuucaagc uaccaaagua uugccuguuc gaauuucagg agaaaaacac uaauuaccgu     3660

cgcuauaugg uguccaacca ggaacuuaag cgggcgaauc aguuguuccu gaccuguaaa     3720

gagacggaau ugguauacaa guacaucaaa ucugacaacg auaauaccca ccuggucggu     3780

aacgagaaga caauauuaag caucuggaau aaacucguga uuuuuaugga gagaacuaag     3840

cugaucgaca agaaacuuuu cgccucccug aagcaauuga aagaugaagu gaaccucauu     3900

aauauccugg acauagcuuc aaagagcauu gacuuuacca aaaucagugc cgggcagaag     3960

aagaucgucc uaaacgauuc ucugucccuu ccgauuaaaa gguggcaguu caagagcgac     4020

gagaaaaugc ucaagcagcu gaaugaugca acauugaucc accaaucgau aacuggcuua     4080

uaugaaaccc gaauugaccu gucaaaacuc ggagaggaau ga                        4122


<210>  3162
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3162
augaaccuga gaagggcccg ggacuacagc aucggccucg aucuugggac cggauccgug       60

gguugggcug ucacagacgc agagggcaau cuguauucuu ucaaggggcg ccccgccaug      120

ggaucacgaa cuuuuccuaa cgcggaacca gcuaguguug cccguauucc gagaggccag      180

aggcggcgcu acgagagaag gcggcaacgc auaaaauugu uacagcaguu cuuuaugagc      240

gauauggaac gaguggaccc cgauuucuuu acgagacuga aucaguccac ccuacucgca      300

gaggacaagg augaaaggau cgccucuugc gcuuggccua uuuucaacuc gucagacuuu      360

acagagaaag auuauuacaa gaaauuccca acuaucuauc accuucgggc auuucuuugu      420

gagagcgacg aaaaggguga ucugcguuug guauaccucg cccugcauaa uauugugaag      480

caccgcggga acuuccugua ucaagacaau cccgcucuca gugccuccga ugcgucuguc      540

gacgaggcag ugagacaguu aguugacgcc cuggcugaau ggugccagaa caaagagauc      600

gagcuuagcu gugaugccgg aaggauaauc gaaguguuug gcgacgcauc cuugcggcga      660

accgauaagc gcgaccagcu gaaaggccua cucgggcugc cugcugaucu ugccaagaca      720

augggaaaag agcuggcguc agccgucuug gguuacgcag cuaauuucag uaagauuuuu      780

cucaucagcg acgccgaaca uaaguucucu cugggcaacg augagaaagu ggaagaguuc      840

gagaccauuu gcccaaauga auguacuucc uuauuuucag cucugagaac agccuacagc      900

gcauaucucc uuagugggau ccugagggac gccaacggaa agaccuuguc uuucugcaaa      960

aagaaagagu acgaucggua ucacgacgau cuagcucugc ucaaggcgcu gguacgcgaa     1020

uacgcacccg aguccuauca gagcuuuuuc ggcggugaaa gacuuguugu gaaugggagg     1080

augacgaaac guuacgaccc uacuaaggcc aagggauaua ccaaauacga ccugguccau     1140

ucggcuggcu cuuacgauga guuggccaag gacgugaaaa agcuccuggg cuccuuagca     1200

gccgaugacu cacgguauca acgaaugaug caggcuuuug augaggaaag auuccugagg     1260

cgccuaaaaa caagugacaa cgggagcaua ccauaccagc uccaccugga ggaacuucgg     1320

gccauuaucg caagacaagg agaguuuuau ccguucuuga aggaagaguc caagaaaauu     1380

gagucucugg ugagcuuuag gauccccuac uaugucgguc cucucacuac caagaaugcg     1440

gcuguugaug ugcacggcaa caaacgcuuc gccuggucag aacgaaagcc agggauggag     1500

agugcaaaag uacggcccug gaacugggac gaugugaucg accguggaaa guccgccgaa     1560

aauuuuauug ucagaaugac aggcaccugu acguaccugc agaacgagaa ggugcuuccu     1620

aaaugcagcc uguuguauga ggaguucugu guucucaaug agcugaacgg ugcucauugg     1680

ucugccgaug gggaccacga augcccauuu gauacuucag acaggcgcgg cauagugcag     1740

gacuuauucu guggaaagcg gagagucagu uacaaacaag uggaggauug gcugaggcag     1800

cauggcagcc gacgcgcacg gguauccggg ggacagggug cuucuggcuu cgaaucgaag     1860

augagcuccu acaucuuuuu cucaaaagac guucuacacg uggauagaau ugaccccgcc     1920

gauuuuccga ugaucgagga gauaauucuc uggaauacac uguucgaaga cagggauauc     1980

cuuaaggagc guaucgaaga ggcguauggg gaccgcuuga acgccgaaca gauuaagacc     2040

aucugcaaaa agcgguuuac uggauggggc agacugagua ggcgacuccu gacagguauu     2100

aaagcucaaa ccgaucaggg ggacuuaucu auaauggaua uccugcggga gggcgacccu     2160

aauaacgauc accgcggaaa ggcaaugguc uucaugggca uucuucauag cgaggacuug     2220

ggguuucaga aaaagaucga ccagaucaau caagaaaaag uguccgccgg aaaggucuca     2280

cucgaggauc ugcccgguag cccagcucua agaaggucua uuaaccaggc caugcgggug     2340

guugacgaaa uaguguccau cgcaaagugc ccucccaguc acauuuaccu ggagguaacg     2400

cgagaugaag acggcgcgag aaaagggcgc aggagcaccc gucgguauga ugccaucaag     2460

gcugcccucg agaaauucaa ggaggaaucu ccaacauggu uugaccagga ugucuucgga     2520

gaguuuagaa ggucccgcgc agaagaccug gaugagcgac uuucauugua cuucaugcag     2580

ggcgguaaaa gucuguauag cgcuacuccc uuaaagcucg agcugcuuca uaccaaucaa     2640

uaccaggugg accacauuau cccucagucg uauauaaagg augacuccuu ugaaaacaaa     2700

gcccugguuu uggcagggga gaaucagaga aagucugacg cccuccugau cgauagcuca     2760

auucggacag cuauggcgcc auacuggagg gcccuacauc aagcaggccu gaugggagac     2820

aaaaaguuca acagucucau guguuccucu aucagcgaua aacgcgugcg uggcuucauu     2880

gcuagacagc uggucgaaac uucccagauc gugaaacuug ccgggaugau guugcaauca     2940

aauuacccgg aggccgacgu acagcccgug aaggcuagcc ugggacacga uuuacggguu     3000

caggcaggcu uuauaaaaug cagggaaguc aacgacuucc accaugccca cgaugcgcuc     3060

auugcuugug aggugggucg cuauaucaac cagugccauc cugggauuua cgacaaucca     3120

aucggauaug cccgagcagu gagagagcug guccggaagc aaaacgaaga gaugaggaaa     3180

uacggccgcc uucccgguag ugccggcuuu auaguugggg gauucaugag aacccacgug     3240

gauguggaca caggcgaagu agucugggau gcugaggaag aacugucuag gauccgggca     3300

uaucuagacu acuccaaugu guuuguuacc cguaugagcg aggaaacuuc gggggcccuc     3360

uggaacgcga cgauuuauuc accucacuac ggaaagacag cuccaacccu gcauaucaaa     3420

cagggcuugg augugcagcg cuacgguggg uauucuagug ccacuuucgc auacuuuacc     3480

cuguaugucg ccgagaaggg aaagaaacga aaggcugaau uacucggcau ucccaucucc     3540

guggccagaa aaauagaggg ugaaccgagc aagcuggacg cauucguugc ucaggacgcc     3600

gaggcgaaaa agcugaaaca cccucugauu aucagggccc ggaucuugaa guacgcacgc     3660

uuugagaugg aaggcaauca auucuauuuu gcugcccagg augcugugua cucugcaaga     3720

cagcucguac ugaaggggga ccuauauagc gucgccgcca aggcuuguga uaagcaucug     3780

aacgacuccu gcauuucaaa ugaggaucuu aucaggcucu acgacgaacu ggugcacaaa     3840

ggagauuugc uguguaacaa guucggcaau gugacagacg ucuuacgggc ggggcgagag     3900

agauacgcag gacucaguau uagcgagaaa cgccagcugg uuugcaacgu gcuugccuau     3960

uacaggucug cuaccgcccg gguagauaua ucccuggugg gcgguccaaa gcaugcaggg     4020

aauuugggaa ucgccuucgu ccucaacaau cccgaagcua uuguguuugu ugaccaauca     4080

gugacuggca uguucgagaa gcguacaaga gucgaacugu ga                        4122


<210>  3163
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3163
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaua acgaaagaga gaaguccucu auaaucaaaa uuggugucag gguuaauccc      120

uugacagugg acgaacagca aaacuucgag aagggcaaau caaucacuac gaaugcagau      180

cggaccuuaa agcgcaguau gcgacguaac cugcagagau uuaagcuaag gaaagaaaau      240

cucauugaga uccugaagaa acagaaguuc auaaacgacg agacaauccu uagcgaaaau      300

gggaacaaau ccacuuuuga gaccuacaau cugcgggcca agucugcgac agaagagauu      360

accuuggagc aguucgcucg cguacuccug augaucaaca agaaaagagg auauaagucg      420

ucaaggaaag ccaagagcca agaugaaggc cagcugauug acgguaugga gaucgccaaa      480

aagcucuaca augauaacuu aacuccuggg caguuugugu uccagauacu gaaagaagga      540

aagaaguaua uuccagacuu uuaccggagu gaucuucaag cagaguucga caaaaucugg      600

uccuuucaga agcaguucaa uccggauauu uugacagaag aguuuaacaa acagaucauc      660

ggcaagggca aagucaacac cacgaagauu uuccuggcua aguaugggau auacacuauc      720

gacaauaaag gagcagauaa gaaaucucaa gccuauaacu ggcgcgugga ggcucuaacc      780

aaucagcuca caaaggaaga acuggccuuu guuauuagcg accuuaacgg uaucauaucc      840

aauucaagug gcuaccuggg gagcauuucu gaccgaucca aagaauugua cuucaacaag      900

cagacugugg gacaauaucu caucgcgcag cugaaggaga auccccacac ccagcugaaa      960

aaccaggucu ucuacagaca agauuauuua gacgaauuua auacaaucug ggagacccag     1020

gcaaaguucc auaaagagcu cacgaacgaa cuuaaggagg aaauuaggga uaucauuaua     1080

uuuuaccagc ggaaacugaa gucacagaag ggcuugauca gcuucugcga guuugaaagu     1140

aaacaaauug agaucaauau cgacgguaag aaaaagcuaa aaacuguggg gucuaaggua     1200

uguccuaaau ccagcccacu guuccagguu uuuaagauuu ggcagauacu caacaauauc     1260

caggugauug agaagucgac aggaaaaucu aaguuccugg aacaagauga gaaagaaauc     1320

cuuuuuaagg agcugaacuu caaagacaag auuuccaaga ccgaugccuu gaaaauccuc     1380

uauaagaauu acaaagagcu ggacuuuaac uucaaggaaa acauagaggg caaucguacu     1440

caggcuacau uuuuaaaagc cuuccaguca aucauugaac ugaguggcca cgaggucagc     1500

gauuucucca agaucaagac cgacgaaauu cuagagaucg ugacgcccau auuugagggg     1560

uucggauuua acaccaaucu ccugcaguuc aacucuaauc uuaaagguaa ggauuuugaa     1620

aaacaagcag uguaucaguu guggcaucug cucuacagcu ucgagggcga caacucaaau     1680

acagggaacg aaaagcugau ucagaaaauc agugagcuuu acggauuuga acgcgaguau     1740

gcuauuauca uagccaauau cuccuuccaa ccugacuacg gcagccuguc uacuaaggcg     1800

auuagaaaga ucuugccaca ccucaaagag gguauugaau uuucacuggc cugcgaguau     1860

gcaggguaca ggcauaguaa gaacagcuua accaaagaag agaagaauaa aaagauccug     1920

aaagauaaga uagacauucu acccaagaac ucccuccgga auccugucgu ugagaaaauc     1980

cugaaccaga ugaucaaugu gguaaacgcu acaauugaga cuuauggcaa gccagaugag     2040

auccgaauug aacuugcccg cgaguugaaa aagucugcua aagaaagaga ggaccugacc     2100

aacgccauaa auaagucgac acucgaucac gagaaguaca aagcacuguu acagaaggag     2160

uucggacuga gcaacguguc caggaaugac aucauucggu auaaacuuua cuuggaacuc     2220

gaacacaacg gcuuucauac ccuguacuca aauacuuaua ucgagccgaa caagcuauuc     2280

aguaaagaau uugauauuga gcacaucaua ccccagucuc gccuguucga cgauagcuuc     2340

uccaauaaga cgaucgaguc aagaagcgcc aacauugaaa aggggaauga gacagcuuuu     2400

gacuacgucu cuaacaaaaa uggagaagug ggucucaagg auuaucugaa aaacaucgag     2460

gaccuuuuca auaacggcaa gauuuccaaa accaaguaca auaaguugaa aaugaagggg     2520

gaugaaaucc cuagugacuu uauagcgagg gaccugcgug auacucaaua uauuagcaaa     2580

aaggcaaaau cuaucuuaga ggaccucguu aaggaugugg ucuccaccgg aggcucaauc     2640

acagaccggc ugcgagagga uuggcagcuu auugacauaa ugaaggaacu gaacugggau     2700

aaauacgaca aguugggucu caccgaggug aucgaaaaca augaugggca uaaaauuaac     2760

agaaucaagg acuggacuaa aaggaaugau caccgccacc augccaugga cgcucugaca     2820

auugccuuca cgaagcggag uuauauccag uaccuaaaca aucugaacgc aagaagcgau     2880

aaaucgggcu ccauauacgg aaucgagcag aaggaacucu auagggacaa gaauaacaaa     2940

cugcgcuuua agccacccau uccucuugac gauuuccgag ccgaggcuaa aaagcaauug     3000

gagaauaccc ugguaucuau caaagccaag aacaagguug caacuauuaa uaucaacaau     3060

accaaaaagc agggcgggga aaacaaaaag aaacaguuaa caccacgggg acagcuccac     3120

cuggagacaa uauacgguag cauucaacag uaugugacua aggaagagaa ggucggcgcg     3180

aauuuuaaca aagagcuuau cgaaaaggug gcuaauaaaa agugucguga ggcccugcua     3240

aaaagacuca ucgaguucga caacgauccc aagaaggcau uuaccaggaa aaacucacug     3300

aauaagaacc cguuguacau ugacacgauc aaaaccuaua aggucccuga aaaagugaag     3360

acaguuacua ccgaaacaau auacacuauu cgcaaagaua ucaaucccga ccugaagauu     3420

gauaaggugg uagacaaaaa gaucaaaaac auauuagagc uccggcugaa ggaguucaau     3480

ggggaugcca aaaaggcuuu uaguaaccuu gacgaaaagc caauuuggcu gaauaaagag     3540

aagggaaucu ccaucaaaag agucaccauu acgggcaucu cuaacgccau ugcauugcau     3600

gauaagaaag acaaggaagg uaauuucaua cucgacaacc aggggaagaa acaagcugug     3660

gauuucguua auacaggcaa caaucaccau guggccaucu auaaggacga gaaaggaaac     3720

cugcaggaaa augucauuac cuuuuacgag gcgguggaga ggguaaacca gggcaucccu     3780

aucauugaua agacuuacaa acagaacgaa ggguggcaau uccuauuuag caugaagcag     3840

aaugaguauu ucguguuucc caacgaaaag acaggauuca auuccaacga gauagaccug     3900

cuuaauccag aaaacuacca ccucaucuca cccaaucugu uucgaguuca gaaaauugcc     3960

accaaggauu auuucuuucg ccaccauuug gagacuaacg ucgagaauau caccacaacg     4020

aaagaccuga ccuggaagcg guuaggcauu aacgguauca uaaauauccu caaagugaga     4080

auuaaccaca ucgggcagau ugugagcguc ggagaauacu ga                        4122


<210>  3164
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3164
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucauaacug augacuauaa gguucccgcu aaaaagauga aagugcucgg uaacacggau      120

aagaaguaca ucaaaaagaa ucuucugggc gcauuguuau ucgacucugg ggagaccgcc      180

gaagcgacaa gacugaaaag gacugcucgg cgccgauaua cccguagaaa gaacaggcua      240

cgguaccucc aggagauuuu ugccgaagag augacaaaag uagaugaauc auucuuucaa      300

cgccuggacg agaguuuccu uagaugggau gacgauaaua agaagcuggg aagguauccu      360

aucuuuggca acaaagcaga cguggucaag uaccaccagg aguucccaac cauuuaucau      420

uugcggaaac accucgccga uagcuccgaa aaggcugacc ugcgccuggu guaccucgca      480

uuagcccaua ugaucaaauu ucgaggucac uuccugauag agggggaacu uaaugcugag      540

aacacugaug uucagaagau cuuugccgac uucgugggag ucuacgacag aacauuugau      600

gacucucauu ugucggagau uaccguggau gcggcaucaa uccugacgga aaagauuagc      660

aagaguaggc ggcuagagaa ucucaucaag uauuacccga cugaaaagaa gaacacccug      720

uucggcaauc uuauagcccu ggcuuugggc cuccagccca acuuuaaaau gaauuucaag      780

cuguccgagg acgccaaauu acaauucucu aaggauagcu augaagagga ccugggggag      840

cuccuuggaa agauugguga ugacuacgca gaucuguuua cauccgcuaa aaacuuguau      900

gacgccaucc uacugucagg cauucucauc guagaugaca auaguacuaa ggcgccucug      960

agcgccucua ugaucaaacg uuacguugaa caccaggagg accuugaaaa gcugaaagag     1020

uucauuaagg caaacaaguc cgaauuguau cacgauauau uuaaagacaa gaacaaaaau     1080

ggguacgcug gauacaucga gaacggcgug aagcaggaug aguucuauaa auaccucaag     1140

aauacccugu caaagauugc cgguagcgac uacuuuuuag auaaaaucga acgcgaggac     1200

uuccugagaa agcagaggac auuugauaac gggaguauac cacaucaaau ucaccuacag     1260

gaaaugcaug cuauccuccg gcgacaggga gacuauuauc ccuuccugaa agagaaucag     1320

gaucgcaucg aaaagauucu uaccuuuaga aucccuuacu augucggccc auuggccagg     1380

aaagacucuc gguucuccug ggcagaguac cacagcgaug agaagauuac gcccuggaac     1440

uuugacaaag ugauagauaa ggaaaagucg gccgagaaau ucaucacucg caugacacug     1500

aaugaccucu accugccuga agagaaggug cuuccaaaac acucucaugu cuaugagacc     1560

uacgcuguuu auaacgaacu gacuaagauu aaauacguga augagcaagg caaggacucc     1620

uuuuucgauu caaacaugaa gcaggaaauc uucgaccacg uauuuaaaga gaauagaaag     1680

gugacaaaag aaaaguugcu caacuaucug aauaaagagu ucccggagua caggaucaag     1740

gauuuaauug ggcuggacaa ggaaaacaaa aguuuuaaug cgagccuagg aaccuaucau     1800

gaucucaaga aaauacugga caaggcauuc cuugaugaca aagucaacga ggaagugauc     1860

gaggauauua ucaagacguu gacccuguuu gaagacaagg auaugauuca cgagcgucuc     1920

cagaaauacu ccgacaucuu cacagccgau caacugaaga aauuagagcg gcgacauuac     1980

acugguuggg gcagacuguc uuauaaacuu auaaacggga ucaggaauaa agaaaacaau     2040

aagaccauuu uggacuaccu caucgacgau ggaagcgcua accgcaauuu uaugcagcug     2100

auuaacgacg auacacuacc cuucaaacag aucauacaga agucacaagu ugugggcgac     2160

gucgaugaca uugaggccgu gguacacgau cugccuggua guccagcaau caagaaaggg     2220

auucuccagu ccguuaagau cguggacgaa cuggucaaag ugaugggcga uaaucccgac     2280

aacauaguca ucgagauggc ccgggaaaau cagacuacca acagaggaag gagccagucu     2340

caacagcgcc uuaagaaauu gcaaaauuca cugaaggagu uaggcaguaa cauucucaau     2400

gaggaaaagc cuagcuauau cgaggauaaa guggaaaacu cccaucugca gaaugaccag     2460

cuuuuucugu acuauauuca gaacgggaag gacauguaca caggagauga guuggacauc     2520

gaucaccucu cugacuauga uauagaccac auuaucccac aggcuuucau caaagaugac     2580

ucgauugaua accgaguucu gaccagcucc gccaagaauc gggguaaauc agacgaugug     2640

cccagucuag acaucguacg ugcaagaaag gcggaguggg ucaggcugua caagucuggc     2700

cucauuagca aacgcaaguu ugauaaccug acuaaagcug aaagaggggg acuuacggag     2760

gccgacaagg caggcuucau aaaacggcag uugguggaaa caaggcaaau caccaagcau     2820

guugcccaga uucuggacgc ucgauuuaau acugaguccg augaaaacga caaggugauc     2880

cgcgauguca aagugauuac cuuaaaguca aaucucguaa gccaguuccg gaaagacuuc     2940

gaguuuuaca aggugagaga gaucaacgau uaucaccacg cccaugacgc auaccugaau     3000

gcuguugucg guacagcccu ucugaaaaag uauccgaagc uagcgucuga guucguguac     3060

ggggaauaua aaaaguacga uauaaggaaa uuuaucacca acuccaguga caaggccacu     3120

gcuaaauauu ucuuuuacag caaucucaug aacuucuuua agcgugugau ucgcuacucu     3180

aauggcaaag ucaucguucg gccuguggug gaauauucca aggauacaga ggacauugca     3240

ugggauaaga aaucaaacuu cagaacgauc ugcaagguac ugaguuaccc ccaagucaau     3300

auagugaaaa agguugaaac ccagacugga ggcuuuagca aagagucgau uuugccaaag     3360

ggggacuccg auaagcugau cccuaggaaa acaaagaaag ccuauuggga caccaagaaa     3420

uacggagguu ucgacucucc cacaguggcu uauagcgucu uugugguugc cgauguggag     3480

aagggcaagg caaagaaguu aaaaacugua aaggaacucg ucgggaucuc aauuauggag     3540

cgaaguuucu ucgaagagaa cccaguggaa uuucuggaga auaaaggaua ccacaacaua     3600

cgggaggaca aacuuaucaa gcugcccaaa uauuccuugu ucgaauuuga gggcgguaag     3660

cgcagacucc uggcgucugc cagcgaacua cagaaaggga acgagauggu gauuccuggc     3720

caucugguca aacuucucua ccacgcucag aggaucaauu ccuucaacuc aaccaaauac     3780

cuggauuaug uuagcgccca uaagaaagag uuugaaaagg uguugaguug uguagaggac     3840

uucgcaaauc uguacgugga ugucgaaaag aacuuaucua aaauucgggc uguggccgac     3900

uccauggaua auuuuagcau cgaggaaaua ucgaacucau ucaucaaucu ccugacgcuu     3960

accgcacugg gagccccagc ugacuuuaac uucuugggcg agaagauucc gcgaaaaaga     4020

uauacaucua cuaaggagug ccucaaugcg acccugaucc accaaaguau uacagggcua     4080

uacgaaacuc gcaucgaucu guccaaaaua ggagaggaau ga                        4122


<210>  3165
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3165
auggagaaca agaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucauaacag augacuauaa aguucccucu aagaaaauga aggugcucgg uaauacugau      120

aaaagauuca ucaagaagaa ccuuauuggc gcucuguugu uugacgaagg gacgaccgca      180

gaggccaggc gguuaaaacg cacagcgcga cguagauaca cuaggcggaa gaaucgccug      240

agauaucuac aggaaaucuu cgcugaggaa augucaaaag uagauaguag cuuuuuccac      300

aggcucgacg auuccuuucu gauuccugag gacaagaaag gaucuaagua cccaaucuuc      360

gccacccuua uagaggaaaa ggaguaucau aaacaauuuc cgacaaucua ccaucugcgg      420

aagcaguugg cagauucgaa agaaaagacc gaccuccgcc ugauuuaucu ggcccucgcu      480

cacaugauca aauaccgagg ccacuucuua uacgaggaua cuuuugacau uaagaacaau      540

gauauccaga aaauauucaa cgaguuuauu ucaaucuaua auaacacauu cgaagguaau      600

agccugagug ggcagaacgu gcaagucgag gcaauuuuua ccgacaagau cuccaagucu      660

gccaaaagag aaagggugcu uaaguuguuc cccgacgaga aaagcacggg acuguucucc      720

gaauuucuaa agcucaucgu uggcaaucag gcugauuuca aaaagcauuu ugaccuggag      780

gagaaggccc cucuucaguu cucacgggau acuuacgacg aagaucugga gaacuugcuc      840

ggccagauug gggacgauuu ugcggaccug uucguggcag ccaaaaaguu auaugaugcu      900

auacugcuca guggaauccu uaccgucaca gacccaagca cuaaagcucc ccugucugca      960

uccaugauug aacguuacga gaaucaccaa aaggauuugg cuacccuaaa acaguuuauc     1020

aagacaaacc ugccugaaaa guaugacgag guguucucag accagagcaa agaugguuac     1080

gccggcuaua uagacgggaa gaccacgcag gagaguuuuu acaaauacau uaagaaccuc     1140

cugucuaaau ucgaaggagc ggauuauuuu cuugagaaga ucgaacgcga ggacuuccug     1200

agaaagcaaa ggacuuuuga uaauggcucc aucccacacc agauucauuu gcaggaaaug     1260

aacgccaucc uccggcgaca aggugagcac uaccccuucc ugaaagagaa uaaggaaaag     1320

auugagaaga uauuaacauu ccgcaucccu uauuacguag ggccacuggc aagaggaaac     1380

agggacuuug cuuggcuaac ccggaauagc gaucaggcca uucgcccgug gaacuucgaa     1440

gagaucguug acaaagccuc gucugccgag uccuuuauca auaagaugac uaacuaugau     1500

cucuaccugc ccgaagagaa agugcuuccu aagcauucau ugcuguauga aacauucgca     1560

gucuacaaug agcucaccaa ggugaaauuu auugccgaag gccugagaga cuaccaguuc     1620

cuugauagua ggcagaagaa agacauauuu uauacgcugu ucaaggcuga ggauaaacgu     1680

aaggugaccg agaaggacau cauucaauac uugcacacag ucgacggcua ugaugggauc     1740

gaacucaaag gaauugagaa gcaguuuaac gcgagccugu ccacuuacca cgacuuacug     1800

aaaaucauaa aggauaaaga guucauggac gauccaaaua acgaggaaau ccuagagaau     1860

auuguucaua cccucacaau cuuugaggac cgggaaauga uuaagcagcg acuggcacag     1920

uaugauucuc uuuucgacga gaaggugauc aaagccuuga cuagaaggca cuacaccggu     1980

uggggcaagc ugagcucaaa acucauaaac gggauucgcg auaagcaaac aggaaaaacc     2040

auccuggacu auuuaaugga ugacggcuac aauaaccgga acuuuaugca gcugauuaau     2100

gaugacgaac uuaguuucaa ggagaucaua aagaaagcuc agguaguggg uaagacugac     2160

gaugucaaac aggugguuca agaauugccc ggguccccug ccaucaagaa aggcauucuc     2220

cagagcauca agcuggugga cgagcuaguc aaagugaugg gacaugagcc agaaucuauu     2280

guaaucgaga uggcaagaga aaaccagacg acagccaggg gcaagaaaaa uucacagcaa     2340

cgcuacaaac gaauagagga uagucugaag auucucgcua gcgggcugaa cgccaaaauc     2400

cuuaaggagc accccaccga caauauccag uugcagaacg aucggcuguu cuuauauuac     2460

cuccaaaaug gacgugacau guauacuggu aaaccgcugg auauuaacca gcuuuccucu     2520

uacgacaucg aucauauugu uccucaggca uuuauaaagg acgauucgcu ggacaauaga     2580

guguugacca gccucaagga caacaggggc aaauccgaua auguccccuc acuggaagug     2640

gucgagaaga ugaaaacauu cuggcagcaa cuacuggaca guaagcucau cucuuaucgc     2700

aaauuuaaca aucugaccaa ggcggaaaga gggggacuug augagcggga caaggugggc     2760

uucauuaaaa ggcaguuggu ugaaacucga cagaucacaa agcacguggc ucagauucug     2820

gaugcccgcu acaacacgga gguaaaugag aaagacaaga aaaaccggac cgucaagauc     2880

auaacuuuaa agagcaaccu cguguccaau uuuagaaaag aguucaggcu guauaagauc     2940

cgugaaauua acgauuacca ccaugcacac gacgccuacc uuaaugcugu uguggccaaa     3000

gcaauccuga agaaauaucc aaagcuagaa ccugaguuug ucuacgguga uuaucaaaag     3060

uacgaccuca aacgcuauau uucacggagc aaggauccca aagaaaucga gaaggcuaca     3120

gagaaauacu ucuuuuauuc uaaccuguug aauuucuuua aggaagaggu gcauuacgcc     3180

gacgggacca uaguaaaaag agaaaacauu gaguacucca aggauacagg cgaaaucgcg     3240

uggaauaagg agaaagacuu cgccacuauc aagaaagugc ugaguuuacc acagguuaac     3300

auugucaaga aaagggaggu gcagaccgga ggcuucagca aggaaucuau acuccccaag     3360

ggaaauuccg acaaacugau cccucgaaag acgaaagaua uucuuuggga caccacaaag     3420

uauggggguu uugauucacc agugaucgcu uacaguauuc uguugaucgc agacauagag     3480

aaaggcaagg ccaagcggcu caaaacuguc aagacccugg uugggaucac aauuauggaa     3540

aaagcuacuu ucgaaaagag cccgaucgcc uuucuagaga acaaaggaua ucacaaugug     3600

cgcaaggaaa acauucugug ccuucccaag uacucccucu ucgagcugaa aaauggcaga     3660

aggcggaugu uggcaucggc gaaggaacug cagaaaggua acgagaucgu guuaccugua     3720

caccucacca cgcugcuuua ucaugccaag aauauacacc gacuggauga accagagcau     3780

uuggaguaca uucaaaaaca cagaaacgaa uuuaaggggc uccugaaccu agucucugag     3840

uucagccaga aauaugugcu ggcugacgcc aaucuugaaa agaucaagaa ccucuacgca     3900

gauaaugagc aggcugacau cgaaauucug gccaacucau uuaucaauuu guuaacauuc     3960

accgcacugg gcgcccccgc ugcguuuaaa uucuuuggaa aggauguuga ccgcaaaagg     4020

uacacuacag ugagugagau acucaacgcc acccugauuc aucaauccau cacuggccuu     4080

uaugagaccc guauugaucu gucuaaguug ggggaagacu ga                        4122


<210>  3166
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3166
auggagacca aguucacaua cuaucugggc cucgacaugg ggacugauag ccuuggaugg       60

gccgugacgg acuccucuua ccacauccug agagcuaaag guaaggauuu guggggcguc      120

agguuauuuc ccucagcaga aaccaguguu gagcggcgca cauaucgagu gaaccguaga      180

aggcggcagc gcgaaguagc cagaauuggg gcgcugaaag agcuauucgc ugacgaaaua      240

gagaaggugg auccuggauu uuucgucagg cucgacgaga gcaaauuuca uauggaagau      300

cgguccgaga auaacaagca aaaguacggc cuguucgccg acaaacuguu uucugaugca      360

gguuauuaca agaaauaucc aacuaucuuc caccuucgca aggaauugcu cgagucggag      420

gaaccgcaug acgugcgacu gguuuaccug gcccucuuaa auauguuuaa acacagaggg      480

cauuuccuga acucaagccu ucagauugag ggagaaaaua guaacaucgc ugaguccuac      540

cagauguuug uggauaccuu gacagacgaa gaucuggacu uccccgagca ggucucuggc      600

gagaggcuac aagaaauuuu ucggagcucc gacucacgua agaagaaagu ggagaaucuc      660

cuggcagaaa ugaaccuuag uaagaaacag aagcgcgaga uccaguucgu aaaucugaua      720

ugcggcuuga aagggaagcu cagcgaacug uucccugaua aagaguauga cgaggaacag      780

aagaagaucg ccuuuucuuu ccaagauucc gacuacgagg auaaagaaaa caccauucag      840

gaguuacugg acgaaucaga gaugaauaug aucuaugcug uuaaggccgu gcacgauagc      900

ggaguccuca gugguauuau gaacggcgag agauaccuuu cuguggcgag gguggcaucc      960

uaugaaaaac acaagaaaga ccugcagauc uugaaggaua uacuaaagaa auacgacacu     1020

aaggaguauu uugaaauguu ccggaaugag agcgccaaau cggagucuua cuccgcuuac     1080

gucgggucag uuaaguauca uggaacauuu cgacgcggcc ugaguggugg gaaacaggaa     1140

gaucucuaca agcacauuaa gaaagugcug ggaaaguucc cacaagacga cgagggcgua     1200

accuauaucc uugaagagau ugaaacggag agcuuucugc ccaaacaguu gacugccucc     1260

aacggcguga ucccuaauca gguccaugca agagagaugc ucgcuauccu gaagcacgcc     1320

gaauuauacc ugccauuccu acaagagaaa gaugacaccg ggcucucugu gagcgaaaag     1380

auucugaaac uuuuuauguu ccagauaccc uauuacguug gaccuuuggg ucaggaguau     1440

aaagauaaga aaggcuacaa cgugugggcg gaaaggaagg ccucagggaa aaucuaccca     1500

uggaacuuug aggacaagau ugaugucaaa aagagugcag agaaguucau cggacggaug     1560

gugcgcagau guacauaucu guccggcgaa caggcucucc cgaaacaaag ccugcuuuac     1620

gagaaguuuc agguacugaa ugaauugaac aaucucagga uacgugguga gaaaauuccc     1680

guuucucuga agcaggaaau cuaucacgac uuauucgggg auggcaaaaa ggugucaaug     1740

acucggcuga ccaacuaccu acuccagaau ggagucauca guggcgagga gaaggacagc     1800

ugcauuuccg ggaucgaugg agguuuccau aacucucugu cgagcguggg caaauuuaag     1860

ggggucuucg gagacacagu gcacaccgau gaacaucgaa aaaugauuga ggacauaauc     1920

uuuuggggca cgguuuucgg ugaugggaag aaacuuuuga aggaaaaggu ggaggaggua     1980

uauggcaauc aacugacuga agcccagaaa aagagaauuu uuggauucaa auuuuccuca     2040

uggggcaggc ucaguaaggc uuuccugaca auggaggggg ccucuaaaga cgacggagaa     2100

aucaaggguu uaauccaggc aaugugggau accaacugua aucugaugga gcuuuugagc     2160

gaccgcuuua cuuacaagga uaaacucaac gaaaugguca caaccgccga gaagccucug     2220

ugcgagugga aaauugaaga ccuagaugag auguaucugu ccgcuccagu gaagcggaug     2280

guuuggcaga cgauaagagu ggucgacgaa gugcaacaga ucauuggcug ucccccuaaa     2340

aggaucuucg uagagaugac ccgcucagau ggggaaaagg gaaaacgaac agugagccgg     2400

cagaagaagc ucguugaccu guacaaagcg cuuggcaagg aggguaaaac uuggucugag     2460

gaauuguccg auaccccaga gagucaguuu cguauuaaga aacuguacuu auauuaccuc     2520

caaaugggga agugcaugua uacaggcgaa gcaaucgagc uggaacagcu uaugaaugac     2580

aaccuguacg auauagacca caucuauccc agacauuuca uuaaggauga caauaucagc     2640

aacaauuugg uccucgugaa aaaggagaaa aacgcccaca agucugacuc cuuuccgauu     2700

gagccugaaa ucaggaacaa aaugaugccc uucuggaagu cacugcgcaa gaaaggauuc     2760

cuaacugagg aaaaguacaa ucggcugacc agaaugagug cuuuuacaga ugaggagaaa     2820

gccgcauuca uaggcaggca gcucguggaa accagccaag ggacuaaggc cauuacgcag     2880

aucuuugagc aggcuuucgc caacucggaa auugucuuuu ccaaagcauc uguugugagc     2940

gcguuccgac agaaguuuga cuguuauaag gugcgcugcg uaaauaacua ccaccaugcu     3000

cacgaugccu accugaauau cgucgcagga aacacauauc gggugaaauu caccucaaau     3060

ccacuuaacu uuauaaagga ggccaaaaag aauccuaaca guagagaaaa uagguacaac     3120

auggacaaaa ucuucgagug ggauguuacu cguaauggcg agaccgcuug gguggcccaa     3180

acaaaggaac aucccgguac cauucaguug gucaagaaau aucugaacaa aaauucccca     3240

uuaaucacua agauggugua cgagaagcac gggggaauua cacagaaagc aacgaucugg     3300

ucugcuaaga aagccgaaag cggcaacacc gacgcguaua uacccguuaa gaugaacgau     3360

ccucgccucg ccgacgugac uaaguacggu ggcguaacaa ccauugcugu cgcaggguau     3420

acacugcuug aguacaaagc cggaggcaag acugugcggu cccuggaagc ucuaccaauc     3480

uaccucgggg gaucaaagaa gcugaccgag gagcagaugg uggaauauuu uagcaauguc     3540

uugcaacagg agaacaaaga aaagguugug agugauguaa ucauuagaaa gaaaaugauc     3600

ccgucuggcu cccugauuaa guacaauggu uucuauuacu auuuagccgg gaaaacggga     3660

gacaggauaa uccucauuag cgcagugcag cugugucuuc ccauggauac caugcuguac     3720

aucaagaaaa uugagaaggc gugcucgaca ggcaacuucg aagagaagga caaaaauggu     3780

aagcaggucu ugucaaaaca acagaacgag uuuguguaug aacucaucau acagaaguac     3840

caacagucua ucuucaaaaa gaagguuggc gccauugggg acacuaucau ugagaaacga     3900

gaaaaguuug cugagaucag ucuggaggaa caguguaaag ugcuaaugca gauauuccug     3960

aauuuucaau ccggaauugg cgucgagcuu aagaucaucg aagggggcaa aaagagcgga     4020

gugaccuacc ucaacaaaaa gguuaugaau gugcaggagc ugaaguugau ugaucagucu     4080

gucacaggug uauucagccg ggaaauagac cuguuaacua ucuga                     4125


<210>  3167
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3167
augaagaaaa gauacagcua uuccaucggc cuggacauug ggaccaacuc ugugggaugg       60

gccgucauaa augaggauua caagguuccc gcuaaaaaga ugacaguguu cgguaacacu      120

gacaggaaaa cgaucaagaa gaaucuccuu ggcaccguac uguuugauuc aggggaaaca      180

gcacaggccc ggcgcuugaa acgaacuaac cguagaaggu auacccggcg cagauacagg      240

uuaugccaac ugcagaauau uuucgcgaca gagaugguga aggucgacga uaccuuuuuc      300

cagcggcuaa gugagagcuu uuucuauuac caggacaagg cuuuugauaa gcacccuauc      360

uucggaaacu ccaaagaaga acgcgccuau cauaagacuu acccaacaau uuaccaccuc      420

cgaaaggacc uggcagauag agaccaaaaa gccgaucuua ggcugaucua uuuggcucuc      480

ucucauauaa ucaaguuucg gggccacuuc cugauugagg guaaacugaa uagugagaac      540

accgacgugc agaagcucuu uaucgcauua guuacggugu acaaucugcu uuucgaagag      600

gaaccgauug ccggggagac uugugaugcu aaagccuugc ugaccgcgaa gacaucaaaa      660

agcaagcguc uagagagucu caucuccgaa uuucccggac agaagaaaaa cggccuguuc      720

ggcaaucuuc uggcauuggc ccucgggcug cgcccuaacu ucaagucuaa uuuuggauua      780

agcgaggacg cuaaacugca gauaacuaag gacaccuaug aagaggaacu cgauaaccuu      840

cuggccgaga uuggugacca uuacgcagau uuguuccuag cugccaaaaa ucuguccgac      900

gcgauccucc ugucagauau ucuuacacug agugacgaga acaccagagc cccauugagc      960

gcaucuauga ucaagaggua ugaggaacac caagaagauc ucgcucuguu aaagaaacug     1020

gucaaggagc agaugcccga aaaauacugg gagaucuuuu ccaacgccaa gaaaaauggc     1080

uaugcugggu acauugaggg aaagguguca caggaagacu ucuaccggua uauaaagccu     1140

auccuaagcc gacucaaagg cggugaugag uuucuggcca agauugaccg cgaugacuuc     1200

cuuagaaaac agaggacguu ugacaacggg aguaucccac accaaauaca uuugaaggaa     1260

cugcacgcaa uucuccggcg ccaggagaaa uacuaucccu uccuggccga acagaaggag     1320

aagaucgagc aacuucugug cuuuagaauc ccuuacuaug uaggaccauu ggcuaaaggc     1380

ggcaauucuu ccuucgcgug gcucaagagg cguagcgaug aaccgauuac ucccuggaac     1440

uuuaaagacg uuguggauga ggaagcaucg gcccaggcuu ucaucgaggg gaugacaaau     1500

uacgacaccu aucugccuga ggaaaagguc uuaccaaaac auucuccccu guacgagaug     1560

uuuacugugu acaacgaacu aacaaaggug aaauauauug ccgagaauau gaccaagccu     1620

cucuaccugu ccgcagaaca gaaggaggcc auaaucgauc accuuuucaa acagacgcgg     1680

aaggucaccg uuaaagacuu gaaggagaaa uauuucucac aaauugaagg acuggagaac     1740

guggauguaa caggugugga aggcgcuuuu aaugccaguc ucgggacuua caacgaccug     1800

uuaaagauca ucaaggauaa agcauuccug gacgaugagg cgaaugcuga aauucuugag     1860

gagauagucu ugauccucac ccuguuucag gacgaaaagc uaauugagaa acgacuggcc     1920

aaguaugcaa accucuucga gaaaagcgug cugaagaaac uuagaaaaag gcacuaccgc     1980

ggauggggcc gguuguccag acagcugauc gaugguauga aggacaaagc cucugggaag     2040

acaauuuuag acuuucucaa agcugaugac uucgccaaua ggaacuuuau ccagcugaua     2100

aaugauagcu cacuugacuu cgaaaagcug aucgaugacg cacaaaagaa agcuauuaag     2160

cgcgaaaguu ugacugaggc cguugcgaac cucgccggcu ccccagcuau caaaaagggc     2220

auucugcaga gccuaaaagu ggucgaugag aucgugaagg uaaugggaca uaaccccgac     2280

aauauaguua uugaaauguc ucgagagaac cagaccacag cacaggggcu gaaaaaugcc     2340

cggcaacguc ucaagaagau caaagaagug cacaagaaaa ccggaucaag gauucuggag     2400

gauaacagug agagaaucac uaaucuuacg uugcaggaca accgccugua uuuauaccuc     2460

cugcaggaug guaaggacau guacacaggc caggaucuug acauaaauaa ccugagccaa     2520

uaugacaucg aucauauuau cccgcagucc uuuauuaaag acaauucuau cgauaaccua     2580

guccucacca cucagaaggc uaaucggggg aagucggaca acgugccuag cauagaaguc     2640

gugagagaua ugaaagacag gguuuggcga cgccaacugg ccaauggagc aauuucccgg     2700

cagaaguucg aucacuugac caaagcggag agaggcgguc uggccgacuc agauaaggcu     2760

agguuuuuac gucgccagcu cguggaaaca cggcagauca ccaaacaugu agcccaacug     2820

cuugacagua gauucaacuc uaagagcaau cagaacaaga aacuggcaag gaacgucaag     2880

aucauuacuu ugaaauccaa gaucguguca gauuuucgaa aagacuucgg gcucuacaag     2940

cugcgggagg uuaauaacua ucaccacgcu caugacgccu accuaaaugc aguggucggc     3000

acagcccugc uuaagaaaua ucccaagcuc gaagcugagu ucguguacgg agauuauaaa     3060

cacuuugacc ugguaaaguu gauuagcaaa ucugauccau cccugggcaa ggcgacggcc     3120

aagguguucu uuuacaguaa cauaaugaau uucuuuaaag aggaauuaag ccucgcagac     3180

gggacccuga ugaagcgccc uguuaucgag acuaacacag aaaccggaga ggucgugugg     3240

gauaaaguga aggacuucaa aacaauuaga aagguccuuu cuuaccccca gguuaauauc     3300

gugaaaaaga cugaaauuca guccggugcu uuuucaaagg agagugugcu gagcaaaggc     3360

aacucggaua aguugaucga gaggaaaaag gggugggacc ccaaaaagua uggaggcuuc     3420

gauuccccaa auaccgccua cucuauauuu guagucgcca agguggcuaa acggaaggca     3480

caaaaacuca agacgguuaa agaaaucgug gguauuacca ucauggagca ggccgaauau     3540

gagaaggaca acauugcguu ccuggaaaag aaaggguacc aggauaucca ggagaagcua     3600

cugauaaaac uuccuaagua uagccucuuu gagcuggaaa auggccgaag acgcuuguua     3660

gcuucagcca acgaguucca aaaaggaaau gaacuggcac ucaguggcaa guacaugaag     3720

uuccuguauc uugccuccag guacgacaaa cugucuagca agauugaguc cgagcagcag     3780

aaaaaguugu uugucgaaca acaucuacac uacuucgaug agauccugga caucgugguu     3840

aaacacgcua cauguuauau uaaggcagaa aacaaucuca aaaagauaau cucacuguac     3900

aagaaaaagg aggccuauag cauuaacgaa caggcgcuua acauguuaaa ucuguuuauc     3960

uucacuucgc ucggggcucc aucuaccuuu guguucuuug acgagacaau ugaucguaaa     4020

cgguacacua ccuccagcga cguauugaac ggaauccuga uacagcaguc gaucacgggc     4080

cucuaugaga caagaauuga ucugucaagg uucggugggg acuga                     4125


<210>  3168
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3168
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagaccu cgauuacaau guucuuagau ucaacaaaaa ggacaugugg      120

gguucuaggc uguuugauga ggccaaaacu gcugcagaac ggcgcgugca acgaaauuca      180

cguagaaggu ugaagcggcg caaguggaga uuaaaccugc uagaggaaau uuucagugac      240

gagauaauga aaaucgauag caacuuuuuc aggcggcuca aggaauccuc ucuguggcuu      300

gaggacaaaa auucgaagga gaaauuuacg cuguucaaug augacaacua uaaggauuac      360

gacuuuuaua agcaguaccc caccauuuuc cacuugcgcg augaacucau caaaaauccu      420

gagaagaaag acauucgacu gaucuaucug gcccuccauu caauauuuaa gagcagaggc      480

cacuucuuau uugaagggca gaaccugaaa gagaucaaga auuucgagac acuuuacaac      540

aauuugauua guuuccugga agacaacgga aucaauaaau ccauugauaa ggacaacauc      600

gagaagcuag aaaagauaau uugugauucu ggcaaggguc ucaaagacaa ggagaaagaa      660

uuuaagggga ucuucaacag cgauaagcag cugguagcga uuuuuaaacu uuccguggga      720

ucaaguguca gccugaauga cuuguucgau acugacgagu acaagaaaga ggaaguggag      780

aaggaaaaga ucucuuuuag ggagcaaauc uaugaagaug acaagccaau uuacuauucc      840

auacucggcg agaagaucga gcuguuagau auugcuaaau cauucuacga cuuuaugguu      900

cugaacaaua uccucagcga caguaacuau auaucugaag ccaaggugaa acuuuacgag      960

gaacauaaga aagaucugaa gaauuugaag uauauuaucc ggaaauacaa caaggagaau     1020

uacgacaaac uauucaagga uaaaaacgaa aauaacuauc cggcauacau cggccugaau     1080

aaggagaaag acaagaagga ggucguggaa aaaucccguc ucaagauuga ugaccugauc     1140

aaaguaauua agggguaucu ucccaaaccu gagcgcauag aagagaagga uaagaccauc     1200

uuuaacgaga uucugaauaa aaucgaauug aagacaaucc ucccaaaaca gagaauuagc     1260

gacaacggaa cccugcccua ccagauacac gagguugaau uagagaagau ccuggaaaau     1320

cagucgaaau auuacgauuu ccuaaacuau gaggagaacg gugugucuac uaaggacaag     1380

cuccugaaaa cauuuaaguu caggauuccu uacuacgucg gcccacuuaa uuccuaucau     1440

aaagauaaag ggggaaacuc auggaucgug cggaaagaag agggcaagau uuugcccugg     1500

aauuuugaac aaaaggugga caucgagaaa agugccgaag aguucauaaa gcgaaugacc     1560

aacaaaugca cguaccugaa uggugaggau gucaucccua aggacagcuu ccucuauucc     1620

gaauacauua uccugaacga gcuuaauaaa guucagguga acgacgaauu ucugaaugag     1680

gaaaacaagc gcaaaauuau cgaugaguug uucaaggaga auaagaaagu aucugaaaag     1740

aaauuuaagg aguaucuccu ggugaaccag auagcuaaua gaacugucga auuaaaaggg     1800

auuaaggaca gcuucaacuc aaauuacgug aguuauauca aguuuaaaga uauuuucgga     1860

gagaagcuga accuagacau cuacaaagag auauccgaaa agagcauccu cuggaaaugu     1920

cuguacggcg augacaagaa gauuuuugag aaaaagauca aaaacgaaua uggcgauauu     1980

cuuaauaagg acgagaucaa aaagauaaac ucuuucaagu uuaauaccug ggggagguug     2040

ucagaaaaac ugcucacagg aauugaguuc aucaaccugg agacugguga augcuacagu     2100

agcguuaugg aggcauuacg gcgcaccaau uauaaccuga uggaacuuuu guccucuaag     2160

uuuacacucc aggagucgau cgauaaugaa aacaaagaga ugaaugaggu gagcuacaga     2220

gaccugauug aagaguccua ugucucacca agucuaaaga gggccauccu gcaaacccuc     2280

aaaauuuacg aagagauaaa gaagaucacg ggccgugugc cgaaaaaggu auucauugaa     2340

auggcucgag ggggagauga gucuaugaaa aacaagaaaa uccccgcccg gcaggagcag     2400

cugaagaaac uuuaugacag cuguggcaau gauauugcga acuuuuccau cgacauaaag     2460

gaaaugaaaa auucauugag cucuuacgac aacaauuccc ugagacagaa aaaguuauac     2520

cucuauuacc ugcaauucgg uaaaugcaug uauacuggga gggagaucga ucuugaccgc     2580

cuguugcaga acaacgauac auacgacauu gaucacaucu auccucggag uaagguuauu     2640

aaagacgaua gcuucgacaa ucucgugcug guccuaaaga acgaaaaugc agagaagucu     2700

aacgaguacc cagugaaaaa ggaaauccag gagaaaauga aguccuuuug gagauuccug     2760

aaagaaaaga auuuuauauc agaugagaag uauaaaaggc ucaccggcaa ggacgauuuc     2820

gaacugcgcg gauuuauggc ccgacaacuu gucaacgugc ggcagacuac aaaagagguu     2880

ggcaagauuu ugcagcagau cgagcccgaa aucaaaauug uguacaguaa ggcugagaua     2940

gccagcucgu uccgugaaau guuugacuuc aucaagguaa gagagcugaa ugacacccac     3000

caugcaaaag augcuuacuu aaacauuguc gccgggaaug uguauaacac gaaguuuacc     3060

gaaaaaccuu acagguaucu ccaagagauc aaggagaauu acgacguuaa aaagauuuau     3120

aacuacgaua ucaaaaaugc gugggacaag gaaaacuccc uggagauagu gaagaaaaau     3180

auggaaaaga acacagucaa uaucacucgc uucauuaaag aggaaaaggg agagcuuuuu     3240

aaccugaacc caaucaaaaa gggugagacc ucuaaugaaa uuaucagcau aaagcccaaa     3300

cuauaugaug gcaaggacaa caaacucaau gagaaguacg gguacuauac aucacugaaa     3360

gccgcauacu ucauuuaugu ggaacacgag aagaagaaca aaaagguaaa aacuuucgag     3420

cggaucacca gaaucgauag uacauugauu aagaaugaga aaaaccugau caaguacuua     3480

gugucccaga agaaacuccu gaauccgaag auaauuaaaa agaucuauaa agaacagacc     3540

cuuauuaucg acucuuaccc uuauacuuuu acgggaguug auagcaacaa gaaagucgaa     3600

cugaagaaua agaaacaguu guaccucgag aagaaauacg aacaaauacu gaagaacgcu     3660

cuaaaauucg uggaggacaa ucagggcgag acagaagaga acuauaaguu uauuuaccug     3720

aagaaaagga auaacaauga aaagaacgag accaucgaug ccgugaaaga acgauauaac     3780

aucgaguuca augagaugua cgacaaguuu cuugaaaaac ucuccucaaa ggauuauaag     3840

aacuacauua auaacaaacu guauacuaau uucuugaaca gcaaggagaa auuuaagaaa     3900

cugaaguuau gggaaaagag ucucauccug cgcgaguucc uuaaaauuuu uaauaagaac     3960

accuacggua aauacgaaau aaaggacucu cagacaaaag agaagcuguu cuccuuuccc     4020

gaggacaccg ggcggaucag auugggccag agcucgcucg gaaauaacaa ggaacugcua     4080

gaggaaucag ucacuggccu guucguuaaa aagauuaaac uuuga                     4125


<210>  3169
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3169
augagcaaca agcccuacuc caucggccug gacauuggga ccaauucugu gggaugggcc       60

gucauaacag augacuauaa aguuccuuca aagaaaauga aggugcucgg uaacacugau      120

aaacacuuca ucaagaagaa ucuuauuggc gcucuguugu uugacgaggg gacgaccgca      180

gaagauagaa gguuaaaacg gacagcccgc cgacguuaca cuagaaggaa gaaccggcug      240

cgcuaucuac aggagaucuu cuuugaagag augaguaaac ucgacagcgg auucuuucau      300

agacuggaug acuucuuucu uauuccagaa gauaagaggg gcuccaaaua cccgaucuuc      360

gcgacccugg cugaggagaa ggaauaucac aagcaauuuc ccacaauaua ccauuugcgg      420

aaacagcucg ccgacucuaa ggagaaagca gaucugcgcc ugaucuaucu caccuuagcc      480

cacaugauua aguaccgagg ucauuuccug uacgaagagu cguuugacau caaaaauaac      540

gauauucaga agaucuucaa ugaguuuaua ucaauuuaug acaacacuuu cgaagggagc      600

agucuuuccg gacagaaugc ucaaaucgag gcaauuuuca cagacaaaau cucuaagagc      660

gccaagagag aaaggguauu gaaacuguuu ccugaugaga aguccaccgg ccuauucuca      720

gaauuucuca aacugaucgu gggcaaccag gcugacuuca agaaacacuu ugagcuugag      780

gaaaaggccc cacugcaguu ccccaaggau acguacgacg aggauuugga aaauguccuc      840

gggcagauug gagacgauuu ugcggaccug uucguggcag ccaaaaaguu auaugaugcu      900

auacugcuca gugguauccu uacuguuacc gacccuagca caaaagcccc acugucugca      960

uccaugauug agcgguacga aaaccaccaa aaggauuugg cugcccuaaa acaguuuauc     1020

aagacuaauc ugcccgagaa guauguggag gucuucucag accagagcaa agacggcuac     1080

gcgggguaua uagauggaaa gaccacacag gaagccuuuu acaaauacau uaagaaccuc     1140

cugaguaaau ucgagggcac cgacuauuuu cuugauaaga ucgaacguga ggacuuccug     1200

cgcaagcaaa gaacguucga uaaugguucu aucccucauc agauucacuu gcaggaaaug     1260

aacgcaaucc ucaggcggca aggggagcau uacccauuuc ugaaagagaa caaggagaaa     1320

auugaaaaga uauuaacuuu ccgaaucccg uauuacgugg gaccccuggc ucgcggcaau     1380

agagacuuug ccuggcuaac aaggaacucc gaucaggcua uucggccuug gaauuucgag     1440

aaaaucguag acaaggccag cucggcagaa gauuuuauca acaaaaugac caauuaugac     1500

cucuaccugc cagaggaaaa gguucuuccc aagcacucuu ugcuguauga gacuuucgcc     1560

guguacaacg aacucacaaa agucaaguuu auugcugagg gccugcgcga uuaccaguuc     1620

cuugacuccg ggcagaaaaa gcaaauagug aaucagcugu uuaaagaaaa gagaaaggug     1680

accaaaaagg auaucauuca cuauuugcau acggucgacg gauacgacgg uaucgagcuc     1740

aaaggcauug agaagcaguu caacgcguca cugaguaccu aucacgauuu acugaaaauc     1800

auaaaggaca agaaauuuau ggaugacagc aagaaugaag caauccuaga gaacauuguu     1860

cauacacuca cuaucuucga agauagggag augauuaaac agcgucuggc ccaauacgac     1920

ucccuuuuug augaaaaggu gaucaaggcu uugacccggc gacacuauac agggugggga     1980

aaacugucug ccaagcucau aaauggcauu ugcgacaaac agacugguaa caccauccug     2040

gauuacuuaa uugacgaugg ggagaucaau agaaacuuca ugcagcugau aaaugacgau     2100

ggccuuagcu ucaagaaaau cauucagaag ucacaaguag ugggagagac agacgacguc     2160

aaacaggugg uuagggaauu gccuggcagu ccagcaauca agaaagggau ucuccagucc     2220

aucaagauag uggaugagcu ggucaaggug auggaccaug cccccgaaag cauuguaauc     2280

gagauggcuc gcgaaaacca gaccacugcc cgggguaaaa agaacucuca acagagauau     2340

aaaaggaucg aggauucacu aaagaaucug gcaaguggac ucgacagcaa cauucugaaa     2400

gagaauccga cggauaacau ccagcuuaag aaugaccgcu uguuucugua cuacuuacaa     2460

aacggcaagg auauguauac aggggaagcg cucgacauua aucagcuguc cucuuacgau     2520

auagaccaca ucauuccuca ggcuuucauc aaagaugacu cgcuugacaa ccgaguucug     2580

accagcucca aggauaaucg gggaaaauca gacaacgugc ccaguuugga ggucgugcag     2640

aagcguaaag ccuuuuggca acagcuccug gauucuaagc uaauuagcga aagaaaguuc     2700

aauaaccuga cuaaagcaga gaggggcggu cucaaugagc gcgacaaggu cggguuuauc     2760

aaacggcagc ugguggaaac cagacagaua acaaagcacg uugcccaaau ccuugaugcu     2820

agguauaaca ccgaggugaa ugaaaaagac aagaagaacc gaacuguaaa aauuaucaca     2880

uugaagucca accuggucuc aaauuuccgc aaagaguuuc gguuauacaa ggugagagaa     2940

auuaacgauu aucaucacgc ccaugacgca uaccucaaug cuguuguggc caaagcgauc     3000

cugaagaagu auccaaaacu ugagccugag uucgucuacg gcgaauacca gaaguaugau     3060

cugaaaaggu acauaagccg uucucgcaac cccaaggagg uggaaaaagc cacggagaag     3120

uacuuuuucu auuccaaucu acucaacuuu uucaaagaag agguacacua ugcugacgga     3180

accauuguga agcgggagaa uaucgaauac aguaaggaua cuggcgagau cgcauggaac     3240

acagaaaaag acuucgccac cauuaagaaa auacugagcu auccacaggu uaauaucguc     3300

aagaaaacag aggaacagac uguggggcaa aacggagguu uguuugauaa uaacauugug     3360

ucuaaggaga aggucguuga cgcuuccaaa cugaucccca uuaagucagg cuuaaguccu     3420

aaaaaguacg ggggauacgc cagaccaacc aucgcauaua gcgugcucgu gacguacaaa     3480

cagaauaaca agauaaagaa uaccauggua ggcaucccgg uccugacaaa acuugaguuc     3540

gaaagggacc ccaaccugua uuugcgaaac cucgguauua auaacguguc gcacguuauc     3600

aagcugguga aauacacugu ccuagaguuu aagcauaaau augggaccua ccggcgcuuc     3660

auugugucca aucaggaacu gaagagagcg aaccaaaucu uuguuacaac uaagcagaug     3720

aaacuuauau cucaguauac cacgcucgau aagaaugagc acgacagccu guugaaaaac     3780

aaucagcaac ugauucaguc aaucuggaac caguucuuag aguuuaugcu cgccacagau     3840

cuggugaaua agaaacagua cgacgaacuu auccugacca acaaucaaac uauugaggaa     3900

uguaggaaca ucaugaaucg gguaauaaca accuugaguu ucacuaacau uaaugcuggc     3960

cagaagaaaa ucauuuuuga ugacacccuc ucccugccua ucaagcgaug gcaguucaca     4020

gauucugaca agauggucaa aaagauaauu gagagcacgc uaauccauca guccaucacc     4080

ggacuguacg aaacuagaau ugaucuuuca aaacucggcg aggacuga                  4128


<210>  3170
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3170
augaaggagu accacaucgg ccuggacauu gggaccagcu ccauaggaug ggccgugaca       60

gauucucagu ucaaacucau gagaaucaag gguaaaacug cuauuggcgu caggcuuuuu      120

gaagagggga agacggcagc cgaacggcgc accuuccgaa caacucguag aaggcugaaa      180

cggcgcaagu ggagauugca uuauuuagac gagaucuuug cgccccaccu ucaagaaguu      240

gaugagaacu uccuaaggcg gcucaagcag ucaaauauuc auccugagga cccagcuaaa      300

aaccaggccu uuaucggaaa gcugcuuuuc ccggaucugu ugaaaaagaa ugaacgcggc      360

uaccccaccc ucauaaaaau gcgagacgag cugccugugg aacagagagc acacuaucca      420

guaacaaaca ucuacaagcu gagggaggcc augauuaaug aggaucggca auuugaccuc      480

cgugaagugu auuuagcugu ccaucacauc gugaaguacc gcggucacuu ccugaacaau      540

gcaaguguug auaaauuuaa gguggggaga auugacuucg auaaaaguuu uaacguccuu      600

aaugaggccu augaagaguu gcagaacgga gaaggcuccu ucaccaucga gcccucuaag      660

guggagaaaa uaggccagcu gcuacucgac acuaagauga ggaaacugga ucggcagaag      720

gcuguagcca agcugcugga aguuaaagug gcggacaagg aggaaacaaa acgaaauaag      780

caaauugcaa ccgccauguc gaaauugguc cucggguaca aggcugacuu ugccacggug      840

gcaaugguga acggaaauga guggaagauc gaucugucaa gcgaaacuag cgaggacgag      900

auugaaaaau uccgcgagga auuauccgau gcucagaacg acauccugac cgagaucaca      960

ucucucuuca gccagauuau gcuuaaugaa auagucccua acgguauguc caucucagag     1020

aguaugaugg auagauacug gacucaugag aggcagcugg ccgaaguuaa ggaguauuug     1080

gcgacccaac cagccagcgc acggaaagaa uuugaccagg uguacaacaa guauauuggc     1140

caggcuccca aagagaaggg guucgaucua gagaagggac ugaaaaagau ccucucuaaa     1200

aaggaaaauu ggaaagagau agacgaacug cuuaaggccg gcgauuuucu gccuaagcaa     1260

cgcauuuccg cuaacggugu aaucccacac cagaugcauc agcaggaguu ggacagaauc     1320

auugaaaaac aagccaagua cuauccgugg cucgcaacag agaaucccgc caccggggag     1380

agggaucguc accaggcuaa auacgaacug gaccaguuag ugucauuccg gaucccuuau     1440

uacgucggac cacuggugac gcccgagguu cagaaggcga cuagcggcgc aaaauuugcc     1500

ugggcuaagc gaaaagaaga cggcgagauu acaccuugga accuauggga uaagauagac     1560

agagccgaaa gugcagaggc cuucaucaag aggaugaccg ugaaagauac uuaccuccug     1620

aaugaggacg uccuuccagc uaacucuuug cuguaucaaa aguacaaugu gcucaacgaa     1680

cugaauaacg uacgcguuaa ugggcggaga cuuuccgugg gaauuaaaca ggauaucuau     1740

acagagcugu uuaagaaaaa gaagaccguc aaagccagcg acguggcauc guuggucaug     1800

gcgaagacga ggggugugaa caaacccucu guugaaggcc ucuccgaucc gaagaaauuc     1860

aauucaaacc uggcuaccua cuuagaccug aagaguaucg ugggggauaa gguagacgau     1920

aaucgcuauc agauggaccu agagaacauu auagaauggc gaagcgucuu ugaggaugga     1980

gagaucuucg ccgacaaacu cacagaagug gaguggcuga cugacgaaca gcgguccgca     2040

cuuguuaaga aacguuacaa gggcuggggu agauugucua aaaagcugcu caccgggauu     2100

guggaugaga acggccaaag gaucauugac cugaugugga auacagauca gaacuuuaug     2160

cagaucguca aucagccugu guucaaggag caaauagacc aguuaaacca gaaagccauc     2220

acuaaugaug gaaugacccu gcgcgaacgg guagagagcg ugcuugacga ugcuuauaca     2280

ucaccccaaa acaagaaagc cauuuggcag guugucagag ugguggaaga caucgucaag     2340

gcaguuggca augcuccaaa aaguauuucc aucgaguuug ccaggaacga agggaauaag     2400

ggagagauaa cccgaagccg ccggacucag uugcagaaac ucuucgagga ucaagcgcac     2460

gaacugguga aggacacguc ucuaacagag gaacuggaga aggugccuga ucucucagac     2520

agauacuacu ucuauuuuac ccaggguggc aaagauaugu acacugggga ccccauuaac     2580

uucgacgaaa ucaguaccaa guaugauauu gaccauaucc ugccacagag cuuuguaaaa     2640

gaugacuccc uugauaauag ggucuugaca ucucguaagg agaacaauaa aaagucggac     2700

caggugcccg ccaagcugua cgcugcaaaa augaagccuu auuggaacca auuacucaaa     2760

cagggacuga uaacccagcg caaguucgag aaucuuacua uggauguuga ccagacaauc     2820

aaauaccgga gccugggcuu ugugaagaga caauuggucg aaacgcgaca ggugauuaag     2880

cucaccgcca acauccuggg uuccauguau caggaggcug ggacugauau uaucgaaaca     2940

agggccggcc uaaccaaaca acugcgggag gaguucgacc ucccaaaggu ucgcgaggug     3000

aacgauuacc accaugcagu agacgcguac cugacaacuu uugccggaca guaucuuaau     3060

agaagguacc cgaaauugcg gucauucuuu gucuauggcg aguacaugaa guucaaacac     3120

gggagugacc ugaaguuacg aaacuuuaau uucuuucaug aacucaugga gggagauaag     3180

ucucagggua aaguggugga ccagcaaacc ggcgaacuga uaacgaccag agaugagguc     3240

gcugacuauu ucgauugggu ucaggaccuu aagcugaugc uagugacaaa agagcucaag     3300

cgcagcaacg ggaaacuguu cgaugccacu auucagaaug caaacuacac caaguugaau     3360

gaaaggaaga aaguauuugu gccccugaag aaagaguuag acaagacacu cuauggaucc     3420

uacucaggca uccagagcuc uuacaugacu aucgucaaaa aguucgauaa aaaggguucc     3480

accuaugcug ugcaacguau uccuguuaug uacaguaagc ugcuugaacu ggagaaagaa     3540

cagagcaagu ugaaccggcu cgugcagucu aucgucacgu ccgacccaca ggcccccaau     3600

aacuuugagg ugcugaugcc uugcguucua ucagggcaac uggugauuga uaauggcaga     3660

aaauucacac uuaccaguua uagguaccag uguagcacug cacagcucuu ucugccaaag     3720

aaagacuuga aguauauaca gaacaauuuc aaggagcuga aaaagacacc cgauaaacaa     3780

gacaagaucu uugaaaccau uuacgacgau aucuuaaaac aggucgacaa guauuucucg     3840

cucuacgaua uuaacaaguu ucgcuccaaa cugcacgacg gaaaggauaa auuccuugcc     3900

cuggagaauu uuggcgaaaa ggagucuaaa gacaagguaa ucacuaagau auugcgaggg     3960

cuccacgcua acgcggaaag aaccagccug aaagagaucg gacuaucaac accgcugggc     4020

caguuccaaa guggugaagg gggaauuacg cuuuccgaua acaccaagau cauuuaccag     4080

ucuccuacug gccucuucga acggcaugug uaucugaaag acuuguga                  4128


<210>  3171
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3171
augagaaaga accaggagcc cuacaauauc ggccuggaca uugggaccag cuccauagga       60

uggucuauca ugaacgauaa uuucgaccuc augaggguga aggguaagaa aggcauuggg      120

guccggcuuu auaacgaagg acaaucagcc gcugagcgcc gaaugcaccg uacagcaaga      180

aggcgguacg gccgcagaaa guggaggcug cgguuguuag aagacuuuuu cgaugagcau      240

auggccgaag uugaugacac guuuuucgcg cgccugaaag auaguaauau cagcccuaag      300

gacgauaaga aauaucgaaa gucccuacuc uuuccaaaau cuaagggugu gacuuaccag      360

gacgaugggg aguucuauaa aaaguacccg accauguauc accugagaua cgcucuuaug      420

acagagcaua ggaaguuuga ccugcgggaa auuuacuugg ccuuccacca uaugguaaaa      480

uaucguggaa acuuucucua cgauacuucg guggacucau ucgaggcaaa gaaucuggac      540

aucaaaggca aguuugauga aauaaacgac cugcucagca guuauaccga uuucuacguc      600

gacaauucca acgccgcuuu aguggagucu auccugcuug agaaaaauac aacccgcaag      660

gauaaaagca agaagauugc aaaauugcug cacguugaag acaaggagaa aggcaagaac      720

aaaaaggcca aggaucuagc uacucagauc uccaaugccg ugcucgggcu gaaaugcaac      780

uuucaccuua uuuucgaacu gcagcaaaag uauucauucg acuuguguag ugagaaaaca      840

gaagagaaua ucgcgaagcu cagcgagguc cuggaugaaa accagaaaau guuacugaug      900

auacucaagg aggugcagga ccagguaaug cuuaacgcau uuguucccuc uggaaugucc      960

cugucagaag ccaugaugca aaaguacgau gacuauggug agcaguugaa aauuuaccau     1020

gaacuagagc acagcgugag ugacgauucu gcugccaagc ugagaggcgc auacagggac     1080

uauaaugaua acaucauuaa acggaucgac ggggauaaua agaaagacac cuucuacaag     1140

cgagucaaga acauccucac gaaaauuucc aaggaguaug uggaucagga cgugcuggaa     1200

agcugcggaa aacuuaagaa acugauagau gagaauaagu uguuuauccg ccagagaacu     1260

gcugccaacg gcguccuccc ucaucaacug caccagauug aaaugaggaa aaucauagac     1320

aaucagaagg aguacuaucc augguuagcg gaacccaacc cuaaugagaa gcggcgcguu     1380

uacucgaaau auaaggugga ggaacugauu gccuucagaa ucccauacua cguagguccc     1440

cuaguggauc cuaacaaugc agacaaaaac aaggaggcua gguuuucuug gaugguccgu     1500

aaaaaggaug gggaaaucac cccauggaau uucuaugaca agguggacug ggccgagucc     1560

gcuaacaauu uuauugagcg gaugaaauca aaggauacau accuccuggg agaagacguu     1620

gugccgaaag agaguaugcu uuaucaaaag uacgaagucu ugaacgagcu gaacaaucuc     1680

cgaaucaacg auagcggccu guccgacucu uucgaagaug ugaaacuuaa gcaggccauu     1740

uauaaugacc uguuuaagaa acagaagaua guaaaaauca cugauuugca gaacuaccuc     1800

guucaaaauc acaaguaucu ggugaaaccc aagauuagcg gcuuagcaga cgagaacaga     1860

uuccugucaa gucuauccac cuacagcgau cucaagacaa ucuuugggga caaagucgau     1920

gacaggaccu acuucaauga uuuugagaag augguggaau auucuacggu cuucgaggac     1980

ggacaugacu acaaccagaa acuggaugaa uauacuuggc uuacaaagga ggaaaaauac     2040

aagaucggua aaaagcgcua ucggggcugg gggaaguugu caaagaagcu gcucaccgga     2100

cugagagaca aaaauaacca cacuauuaug gauaauuuau gggagacaaa caggaauuuc     2160

augcagauac agaccgccga cgaguuuagu aagcaaaucg cugaagagaa cgaacgccau     2220

cugaaaggca gcguguccga ugcgauuaau gacauguaca cgucuccugc aaacaagaag     2280

gccauccgac agguucuucg ggugguagau gacauucaga aagcuauggg uuaugcccca     2340

ucgagcaucu ccuuggaguu cgcacgugaa gaugggcccu cagucagaac cgugaguagg     2400

gccaaccgca ugaagucuau auacgagaaa uacgcuagcg agguuuccga agaggugaug     2460

aaggaccucg auggcgucau caaagacaag aagggacuga augaccggcu auaucuguac     2520

uuugaacagc aaggcaaaga uauguauuca gggcacccuc ucgacuucga uaaggugauu     2580

agcggacagg aguacgacau cgaucauauu cugccacagg ccguaaucaa agacgauucu     2640

cuugacaaca gaguguugac aacuaaggca cugaauaacg auguuaaauc caaggguguc     2700

cccuguagga uguuuaaugg caugcacagu uucuggaaga acuuauauga caaaggguuu     2760

auaagccgac gcaaguucaa uaaccucacc acaaauccgg agaacauuga uaaauacaag     2820

augaaaggau uugugaaucg gcagcuggug gaaacuagac aaaucauuaa acuugucgcg     2880

aacguucuga augacaagua ucagaacgau gacguggaca ucauagaggu gagggcugaa     2940

uugacccacg auguacguaa acauuucaag uuuuacaaaa aucgcaacgu caacgacuac     3000

caccaugccu ucgaugcaua ucucacaucu uuuaucggcc acuaccuguu caagaaauau     3060

ccuaaucuac ggccccuguu cgacuacaac gauuuuauga agguguccga caauguuuuc     3120

aaacagcuca gagguaacaa uuuucugggg gaguuccuua acaagaccgg cgauauuauc     3180

ucaacugacg gaaauuuugu guugaacaag gaagagauga uuaauaaacu gaacaaggcc     3240

uaugcuuuca aaaagaucuu agucacgaaa gaggugggcc aaaggacagg ggccauguuu     3300

aaugaaaccc gauacccagc accuaacagu cggaaggcug cccucaagua ucgcagaaug     3360

cccaaaccag auagccugau aucgguuaag gacuacaaaa auacugauau uuacggaggu     3420

uauuccggca agaacgacgc guacauggug aucguagaua uggggaaaca guauaugguc     3480

gugggagugc ccgucaggua caccgagaaa cuugacaagc ugcggaucaa acacucugaa     3540

cuauaucgag acgagcucag aaagguucug agccgcgaua aaacauugcu ggacucaaag     3600

ggcaauguga aaagguucga uauuguauua gaccgugugc ucuacgguca gcugaucgaa     3660

gaugggaacg agcuuuuuac ccugggcagu uccaaguaua agcggaauuu cagacaguug     3720

uuucucgaca aacaaugcau ugagauacug gauucuagcg ccacuccuca gccaacagac     3780

gaggaacuaa ucugggucua cgaucagauu cuggacaagg uggauaaaua cuucgaacuu     3840

uaugacauca acaggcagcg cgagacgcuc cgaaagggca gagcugcauu cuguucccug     3900

ccgaacauuu caaugaaaaa ggacgauguu accaaaaaga agaucuugaa ugaaauacug     3960

guggccuuac augcuaacga gagcgagagu aauugcgaaa agaucggaaa gaaagucuuu     4020

gggcggcucc aagugcccgg aggcauuaag cugucuaaag aggccaaacu uaucuaccag     4080

uccccuacug gucuguucga aaggguugug uauuugaagg accucuga                  4128


<210>  3172
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3172
auggagaaca agaaauacag caucggccug gacauuggga ccuccucuau aggauucgcc       60

guggucgaug aaaauaacag aguuaugagg gugaagggua aaaaugcuau cggcguacgg      120

cucuuuucag aggggcagcc cgcagccgac cgccgaaguu uccguacaac uagaaggcgg      180

cuuagccgca gaagguggcg gcugcgcuug uuacgagaaa uuuuugagcc uuauaucaac      240

ccaguggaug aagcguucuu uaugagacug aaggagucca aucuaucucc gaaagacgcu      300

aacaagcacu acucgggaga uauucucuuc aauaaccaaa cggaccagga uuuuuaugag      360

aaguacccca ccaucuauca ucugaggcac gcccuuauga cagaacaucg gaaauucgac      420

guccgugaga uauaccuggc aauccaccau auugugaagu uucgcggcca cuucuugaau      480

gccgcuucag caagcaacuu uacuguuggu aaacucaauc uggaagggaa guucgaugcc      540

cugaacgaga ucuaucagag agugcucacc gacgauccuu uugcuuuaag gacagacaau      600

cuggaucaca uuaaagagau ccuuauagac aacaagcgga gucaggccga ccgacaacgc      660

accuugacug gagaugucua ccaguccucu gaagagaagg aaauugagaa aagaaauaag      720

gcgaucgcaa cagaaauucu gaaagccgcu gugggcaaca aggccaaacu aaacguaauc      780

accaauguug agguggacaa ggaggcagcu aaagauugga gccucacguu cgacuccgaa      840

acuaucgaug acgagcuggc caaggucgau ggccagauga ccgacgaugg gagggaaauu      900

auagagaucc uucggucacu guacagugga auuacauugg cggccaucgu gccagaaaac      960

cauacucuca gccagucuau gguggagaag uauaauuccc acaaagcaca ucugaaguua     1020

cugaaagacc ucauaaacaa uaugaccgau acaaagaaag cuaagaaccu ucgcgccgcu     1080

uacgacgguu auauugacgg cgucaagggg aaaguucuga ccagagauga cuucuacaag     1140

caaauccagg gaaauuugga ugacucagcc gaggcacaga aaauccagga ugugauggac     1200

caagaugaau uuaugcccaa gcagaggacg aaagccaacg gcagcauucc ucaccagcua     1260

caacagcagg agcuggacca gaucguagaa aaucaaaagg aguauuaccc auggcucgcu     1320

gaagagaacc ccaaugagac ucgucggggu guggcgaagu auaaacugga ugaacuuguc     1380

acauuccgag ugccuuacua cguugggcca augauuaccg cagaggacca ggaaaagcag     1440

aguggagccu cuuuugcuug gaugaaaaga aaggagccgg gcaacauaac ucccuggaau     1500

uucgaucaga aaguggacag gauggccucc gcaaaccaau uuaucaagcg caugacaacc     1560

acggauaccu aucuguuggg cgaggacguc cucccugccc agagccuguu auaccagcgg     1620

uucgaagugc ugaaugagcu aaacaagauu agaaucgaca ggaaaccaau cucgauugaa     1680

cucaagcagc aaauauuuaa cgaucuguuc aaaaaguuua aaaauguaac aaucaagcac     1740

cuucaggagu auuugguugc uacuggggga uacgacaaac gcccccgaau ugauggucug     1800

gccgacgaaa agcgguucaa cucuggccuc uccgcauauc augaucugca cgggauccuu     1860

ggagcggagg uggucgacgc uaaugauaag caggacgauc uggagaaaau uaucgaaugg     1920

ucaaccauau uugaggacaa gaaaaucuac cgugccaagu uggaugaacu cacauggcug     1980

acugacaguc agaaagagaa guuagcaacc aagagauauc aaggcugggg uaggcugagc     2040

aaaaagcuac ucuccgggcu gcgcaacucu gaucaucgga gcauucuuga caucuugugg     2100

auuacaaaug aaaacuucau gcagauccag gccgacccug auuuugcuga gcuggugaaa     2160

aaugccaacc aaggcaugcu cggaaagaau aacucacagg acgucauaaa ugaucuguac     2220

accaguccac agaacaaaaa ggcaauuaga cagaucuuac uggugguuca cgacauucaa     2280

aaugcuaugc auggccaggc ccccgcgaag auccacgugg aguucgccag gggagaagaa     2340

cgaaacccgc gccgguccau acagagacag caccaaaucg aagcugcaua cgauaaagua     2400

acuagcgacc uuuuggucga ugccggggug aggcaggagc ucaaggacgc uauuaaugau     2460

aaacgugacu ucaaggaucg ccuguuucua uauuucaugc aggguggccg ggacaucuac     2520

aacggggaac cuauuaacau cgacagacug ucuucauaug auauagacca uauucucccc     2580

caggccuuug uuaaagauga cagucugaau aacagggugc uuacggaugc agagggaaau     2640

cgacgcaaga gcgacgcggu cccauccgag uuguucggcg ccaagaugcg gucuacaugg     2700

gaacugauga gagagcaagg uuuaaucucg aaagcuaagu acaggaaccu caccaugaau     2760

ccugaaaaca ucaaugccca cacugagaac ggguuuauua aucggcagcu gguggagacc     2820

cgacagguaa ucaaacuugc agugaacauu cuggcugaug aauauacaga ggacacccaa     2880

auaguuagcg ucaaggccga uuugucccau cagaugagag aagacuucga gcuccugaaa     2940

aaucgcgaug ugaacgacua ccaccacgca uuugaugccu aucuagcugc guucaucggc     3000

aauuaccugc ucgaaaggua ccccaagcug cagucauauu uuguguacgg agacuucaaa     3060

aaguuuaguc agaaggacac uaaaaugcgu cgguucaacu uuauuuauga ucuuaagcau     3120

ucugaccaaa ucgccgauaa aaauggcaag aaaauuuggc acaagacaaa cgacuuggau     3180

uacaucagac aucuguucgc auauaagaaa auauuaauca gccacgaggu cuccgagagg     3240

cgcggggcuc ucuacaacca gacgguuuau aaggccucag acgauaaagc cagcggacag     3300

gguucuaaga aacugauucc aaucaaggcu aauaagccca ccgaaauuua cggcggguac     3360

acuggaaaaa cacuugcaua uauggccauc gugaagguga uaaaaggcaa gaaaauugcg     3420

uacaagguag ucggugugcc uaccuccgcu cugguucagc uagccgggau cgagcauggc     3480

agucaagagg agcucaaccg acuguaugac guguugaagc cacaguucac acagcugaaa     3540

aagaauaaca aaacuggaga aaucagcgag accgucucua aguuugagau ugugauaccg     3600

agaguuaaau accagcaauu aguggaagau gacgauaaac ucuucaugau cggcuccgac     3660

acguaucccu acaaugcaca gcagcuggua cuuucagagg gggcccugaa ggucauuaac     3720

aauguguauc ggaagggaga aaacagugau aagcaggaga gcuacaaauu ggacgacgug     3780

uacgaugaaa ucauuaucgc uaugaagaaa uauuuuccuc ucuacgacaa uaggggcuuc     3840

cgcgagaagg ucgcagaggg uaaaucgacc uuugaaaacc ugccaauauc caaugaguuc     3900

aagaacggga agcuaacaaa aaucggaaag aaagaaguua uuaaucguau gcugcaaggc     3960

cuucacgcca acgcgacuau gaccgaucuc aagauccugg guauuaaauc ucccuuuggc     4020

uucaugcagc agccuacagg gaucgcuuug agcgagaaug cccaacugau auaucaguca     4080

ccaacugacu uauuugagag acgggugcag cucaacaaga uuaaguga                  4128


<210>  3173
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3173
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggaugggc ccugguguac       60

gaguccgaaa acacugauga gaagucuuca auagucagau uggguguuag ggugaauccc      120

uuaaguguag acgaaaaagg caacuucgag aaggggaaag cuaucacgac caaugcagau      180

cggcagcaaa cacacggagc ccgcauuaac cugcagcgau auaagcuacg uagagaaaau      240

cucaaggacu gccugcagaa acagggcugg aucgguacug aggcgcuuug ugaggaaggg      300

aagaccagca cauuugagac cuacaugcug agggcuaaag ccgcagaaga ggagauuucc      360

uugcaugaac ucugccgggu gcuguucaug augaacaaga aacgcggaua uaagucuaau      420

agaaaggcca acaauaaaga ggauggcaag cuguuugacg gcaugacugu cgcuaaaaag      480

cucuacgagg aacacuuaac uccugcagaa uauucguucc aacugcuuuc aagcgggaaa      540

aaguuuaaac aggaguacua uaggagugau uugaaggagg aauuugaccg gaucuggaac      600

gagcagcaga aguacuaccc agaaauacug accgaugacu uuaaacaaca gcuacaggga      660

aaguccaaag guaauacguc uaagaucuuc cucggcaaau augggauuua cagcgccgau      720

cugaagggac uugaccgcaa gcugcagccg uugaaauggc gaguggaggc ucucaagcaa      780

cagguugaua aagaagugcu ggccuuuguc aucuccgacu uaaacggcca gauugcguca      840

acaagugguc ugcucggggc aaucagcgac agaucuaagg agcuuuauuu caauaaacaa      900

accgugggac aguaccugug gacauccuug aaggagacuc cccauguauc aauaaagaac      960

aaaccuuuuu auaggcagga uuaccuagac gaguucgaaa agauuuggga aacccaggcc     1020

acauaucaca aagagcugac cccagaacuc aagcaagaga uccgggauau uaucaucuuu     1080

uaccagcguc cccugaaaag caagaagggc cuuauuagug uuugugagcu ggaacagcgc     1140

aaagugaagg ucacgauaga cggcaaagag aaggaaaucu cuauugggcc uaaaguggcu     1200

ccaaagucca gccccauguu ccaggaguuc agaaucuggc aaaauuugaa caacauacuc     1260

cugauugaua aaggaaauaa cucgauccau ccuuuauacg aggaagagaa gaaucugcua     1320

uaugaggaac ucucugugaa ggaaaaacug uccaaggccg aggcacuuaa agucuugaac     1380

aaaaauucaa aacaguggga ccugaacuac aaggagaucg aagguaauag gacucaggcu     1440

guucucuaug auugcuacaa ccggauuauc gugaugacag gccacgagga auauaauuuu     1500

aagaaacuga aggccaguga gauucgaagc uacguaaaaa ccacuuucac agggcuugga     1560

uuuaacaccg acauacugga guucgauucc ucuuugacgg gccacgaacu cgagaagcag     1620

ccaauguauc aacuguggca uuuacuguac agcuacgaau cagacaauag ucgcaccggu     1680

aacgaugcgc uacucagaaa acuggagaca gccuuugggu ucccgaagga auaugcagcu     1740

gugcuuuccg acgucguguu ugaggacagc uacggcaauu ugucuguuaa ggccaugagg     1800

aaaauccugc cccaccucca ggcuggacug aacuauucac aggccuguga agaggcaggc     1860

uacaagcaua gucggcgcag cuuaacuaaa aaugagcugg aucagaagga auauaaagag     1920

aaacuugaau ugcucccuaa gaacucccug cacaacccag uggucgagaa aauucuaaau     1980

caaaugauca acgugaucaa ugccguaauu gacgaguacg ggaagcccga ugaaauaaga     2040

aucgagcugg cuagggaacu caaaauugga gcggcagagc gugaaaacau gacccggucu     2100

aucaaucagg guaacgccga gaauaagcga auuagagaga uccuggaaaa agaguucgcu     2160

cuuucguaua uaagcaggaa cgacaucauu aaguacaaau uguacgagga acugaagucc     2220

aauggcuuua agacauuaua uucagauacu uacaucagua aagacaaacu cuucucuaag     2280

gauuuugaca uugaacacau cauaccuaag gcccgccugu ucgaugacag ccuuuccaac     2340

aaaaccauug aggcacggga uguuaauauc gccaagucaa acaagacagc uuuugacuuc     2400

auuagcgaga aauaugauca gcaagggauc gaagccuaca agaaaaagcu ggacauauug     2460

cuccaggacg gaucuaucuc caaagcaaag uauaauagac ugcuaaugaa ggaggcggau     2520

auuccaagug gcuucaucga cagggaucug cgcgacaccc aguacauugc uaaaaaggcc     2580

uaugaaaucc ucagcgaucu ggugcgaaac gucacuccca cgacaggugc agugaccucu     2640

cggcuucgug aggacuggca guuggucgau gugaugaaag aacugaauuu ugagaaguac     2700

caaaaauuag ggcucguuga ggugauagaa gacagagaug gcaggaagau uaagcgcauc     2760

aaagacugga cuaagcggaa cgaucauaga caccaugcca uggacgcucu gaccaucgcc     2820

uucacaaaac cguccuacau ucaguaucuu aacaaucuga acgcaagguc agauaagaau     2880

aguagcaucu acggaauuga gaacaaagaa cuacaccgag aggacaauaa gcuccgcuuu     2940

aacgcuccuc ugcccauaaa ugaguuccgg gccgaagcga gaaggcauuu gaagucgauc     3000

cugguauccg ucaaagccaa gaacaaagug augacccaga auguuaacgu gauuaagacu     3060

aaacacggcg ucaucaagaa agugcaguua acaccacgug gggagcucca caaugcuacg     3120

uuuuauggaa ccaagaagcg cccuauuauc aaaaugguaa aggugggugc agcccuggac     3180

gaaguuacua uaaagaaggu cucuagcuca uucauccggg aggcacuucu ggauagauug     3240

caugaauaca acggcgacgc caaaaaggca uuuacaggga ggaauaguau ugauaagaac     3300

cccaucuaua uugacgagga gcacaaaaag accgugccag cgcucgugaa aacaaucgaa     3360

ugggagaugu accaucccac ucgaaagcug auauccccug aucuaaaaau ugacaagguc     3420

aucgauaagg gaauccggga aauucugaaa acccgccuug ccaaguucaa uggcgacgcu     3480

caaaaagccu uuucuaaccu cgaugagaac ccaauauaua aggacgaagc uaaaaagauc     3540

acgcugaaga gaguuagcuu ggagggugug uccucaguga uuccgcugca caccuuaaaa     3600

gauagggagg ggaagcucgu agucggcaaa gacggaaagc ccgugcuggg cgacuacguu     3660

cagacaagca auaaccacca uaucgcuuuc uacauggaug aaaaugggaa ccuucaggac     3720

aaugugguca guuucuucga ggccgcagaa cggaaaucuc agggagugcc uguuauugau     3780

aagaacuaua aucacgacaa aggcuggaac uuucuguuca cuaugaagca aaaugaguac     3840

uucguguuuc caaacgaagu aaccgguuuc aaucccuccg aggucgauuu gauggacgag     3900

gccaacuaua gcaucauauc gccuaaucuc uaccgagugc agaagauguc aaaggcuucu     3960

gggagagauu auugguuucg ccaucaccug gaagcgaugc uaaacgacga uccaaagcug     4020

aggaauagua uguucaaacg uaucacaucc cuuaaggccc ucgagggaau ugugaaaguc     4080

cggaucaacu gcauuggcaa gaucguugca gugggugaau acgacuga                  4128


<210>  3174
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3174
augagcaaca aguccuacuc uaucggccug gacauuggga ccaauucagu gggaugggcc       60

gucauaacag augacuauaa aguucccagu aagaaaauga aggugcucgg uaacacugau      120

aaacacuuca ucaagaagaa ucuuauuggc gcucuguugu uugacgaggg gacgaccgca      180

gaagauagaa gguuaaaacg gacagcccgc cgacguuaca cuagaaggaa gaaccggcug      240

cgcuaucuac aggagaucuu cagccaagaa auuuccaaag uagacucuuc guuuuuccau      300

agacucgaug acuuuuuccu ggugccugag gauaagaggg gaucaaaaua cccaaucuuu      360

gcgacccuug ucgaagagaa ggaguaucac aagaaauucc cgacaauaua ccaucugcgg      420

aagcacuugg cugacagcaa agaaaagacc gaucuccgcc ugaucuaucu ggcccucgca      480

cauaugauua aauaccgagg ccacuuuuua uacgaggaaa guuucgacau caagaauaac      540

gauauucaga aaaucuuuuc cgaguucaua ucuauuuaug acaauacuuu ugaggguaag      600

agccuguccg ggcagaacgc ccagguggaa gcuaucuuca cagacaagau uucaaaaagu      660

accaagagag agaggguucu uaaauuguuc cccgaugaaa agagcacggg acuguuuucu      720

gaguuccuaa aacucaucgu gggcaaucaa gcagacuuua agaagcacuu cgaucuggaa      780

gagaaagccc cucuucaguu uuccaaggac acuuacgaug aggaccugga aaacuugcuc      840

ggccagaucg gggaugacuu cgcugaucug uuuuuagucg ccaaaaagcu guaugacgcg      900

auucuccuuu caggaauacu gaccgugaaa gauuugagca caaaggcacc acuaagugcc      960

ucuaugaucg agcgguacga aaaucaucag aaggaccugg cugcccucaa acaauucauu     1020

cagaacaauc ugcaggagaa guaugacgaa guauuuuccg aucagagcaa agacgguuac     1080

gcaggcuaua ucgaugggaa gacuacccaa gaggcuuucu acaaauacau aaagaaccuu     1140

cugucgaagu uugagggagc cgacuauuuc uuggauaaaa uugaacguga ggacuuucuc     1200

aagaaacagc gcacauucga uaauggcucu aucccccacc agauccaucu gcaagaaaug     1260

aacgcgauua ucagaaggca gggugagcac uacccuuucu uacaggaaaa caaggagaaa     1320

auugagaaga uacugaccuu ucggauccca uauuacguug ggccccuagc ccgaggaaau     1380

ggcgacuucg cauggcucac gcgcaacucc gaucaggcua uuagaccuug gaauuuugaa     1440

gagaucgugg accaagccuc aagugcugaa gauuucauca acaaaaugac uaauuaugac     1500

cuguaccuuc cagaggagaa ggucuugccg aagcacagcc ugcucuauga aacauuugcc     1560

guguacaacg agcugaccaa agugaaguuc auugcagaag gccuuacuag guaccaguuu     1620

cuggauaaaa agcagaaaaa ggacauauuc gacacauuuu ucaaggccga gaauaaacgg     1680

aaggucaccg aaaaagauau cauucauuau uugcacaacg uugacgggua cgauggaauc     1740

gagcucaagg guauugagaa acaguuuaau gcuucccugu cuacguauca ugacuuacug     1800

aagaucauaa aggauaaagc guucauggac gauagcaaga acgaagagau ccuagaaaau     1860

auuauccaca cccucacaau uuuugaggac cgcgaaauga ucaaacaaag acuggcacag     1920

uacgauucac uuuucgacga gaaggugaua aaagccuuga cuaggcguca uuauaccggc     1980

ugggggaagc ugagugcuaa gcucauuaac ggaauccggg auaaaaaguc cggcaaaaca     2040

auucuggacu acuuaaucga ugacggugag auaaaucgaa acuucaugca gcugauccac     2100

gacgaugggc uuagcuuuaa ggaaauuauc cagaaagccc aaguauucgg caagacuaau     2160

gacgugaaac aggucgugca ggaguugccc ggaucuccug caauuaagaa gggcauccuc     2220

cagucaauaa aaauuguuga ugaacuggug aaggucaugg ggcacgcccc agagaguauc     2280

gugaucgaaa uggcuagaga gaaccaaacc acagccaggg gaaagaagaa cagccagcag     2340

cgcuauaaac ggauugagga cucccuaaag aaucuggcau cuggucucga uucgaacauc     2400

cugaaggaaa aucccaccga caacauucaa cuucagaaug auagauuguu ucuguacuac     2460

uuacagaacg gcagggacau guauacuggg aaaccucucg agauaaauca gcugagcaac     2520

uacgauaucg accauauuau cccacaagcg uucauuaagg augacucccu ugacaaucgc     2580

guacugacgu caaguaaaga uaaccgagga aagucugaca auguucccag cuuggaagug     2640

gucgagaaaa ugaaggcuuu uuggcagcag cuccuggauu ccaagcuaau cucagagcgg     2700

aaauucaaca aucugacaaa ggccgaacgu gagagaggcg gucucaacga acuggacaaa     2760

gugggguuua uaaagaggca gcuugucgag acccgccaaa ucacuaaaca cguggcacag     2820

uucuuggaug cccgguuuaa uaaggaaguu accgagaagg acaaaaagaa cagaaacgug     2880

aaaauuauca cacugaaaag caauuuagua ucuaacuuca ggaaagaguu uggccucuau     2940

aagguccgag aaauuaauga uuaccaucac gcucacgacg ccuaucugaa cgcagugcuu     3000

gcuaaggcca uccugaaaaa guacccgaaa cuagagccug aguucguuua uggagauuac     3060

cagaaguacg accuccagcg cuauauaucc aaaagucggg aacccaagga aguggagaaa     3120

gcgacccaaa aguacuucuu uuauagcaau cuguugaacu ucuuuaagga ggaaguccau     3180

uacgccgaug gcacuauugu gaaaagagag aauaucgaau auucuaagga cacaggggag     3240

aucgcuugga acaaagaaaa ggauuucgca acggugaaaa agguacuguc cuuaccacag     3300

guuaauauug ucaagaaaac cgaggugcag acuggagguu uuucaaagga gaguauacuc     3360

ccuaaaggca acagcgacaa gcugaucccc aggaaaacaa aggaaauucu uugggacacc     3420

acaaaguacg ggggauucga uucgccagug aucgccuauu ccauucuguu gaucgcugac     3480

auagagaaag gcaaggccaa aaagcucaaa acugucaaga cccugguugg uaucacgauu     3540

auggaaaagg caaccuuuga gaaaaauccc aucacauucc uagagaacaa gggguaccac     3600

aaugugcgua aagaaaacau ucugugccuu ccuaaguacu cucucuuuga gcuggaaagc     3660

ggccgccgga gaauguuggc gucagccaaa gagcugcaaa agggaaauga aaucguguua     3720

ccaguauauc ucacuacccu gcuuuaccau aguaaaaacg uccacaagcu ggaugagccg     3780

gagcauuugg aauauauaca gaagcacagg uacgaguuca aagaccuccu gaaccuagug     3840

uccgaauuuu cucagaagua uguucuggcu gaugccaauc uugagaaaau uaagaaccuc     3900

uacgcagaca augaacaggc ugauaucgag auccuggcca acagcuucau uaauuuguua     3960

acauuuacug cacugggcgc ccccgcugcg uucaaauucc ucgggaagga cguggaucga     4020

aagcgguaua ccacggucuc cgagauccug aacgccacac uuauacacca aucaauuacc     4080

ggacuguacg aaacucgcau cgacuugagc aaacuaggcg aggauuga                  4128


<210>  3175
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3175
auggagaaga aauacagcau cggccuggac auugggacca acuccguggg augggccguc       60

auaacagaug acuauaaggu ucccucuaaa aaguucaaag ugcucgguaa uacuaacaga      120

aagucaauca agaaaaaucu uaugggcgcu cuguuguuug auagugggga aacggcagag      180

gccaccaggu uaaagcggac agcgcgccga cguuacacua gaaggaaaaa ccggauucgc      240

uaucugcagg aaaucuucgc uaaugagaug gccaagcuag acgauagcuu uuuccaaaga      300

cucgaagagu ccuuucuggu agaggaagac aaaaagaacg agaggcaccc uauuuucgga      360

aaucuugcag augaaguggc cuaccaucgg aacuauccaa ccaucuacca ucugcgcaag      420

aaauuggcug acucuccgga gaaggcagau cuccgacuga uauaucuggc ccucgcucac      480

aucauuaaau uuagaggcca cuucuuaauc gaggguaagc ugaaugccga aaacucggac      540

gucgcgaaac uguuuuacca guugauucag acauacaauc agcuguucga ggaaucaccc      600

cuagaugaga ucgaagugga cgcaaagggg auacucagcg ccaggcugag uaaauccaag      660

cggcuugaga agcugauugc uguuuuuccu aacgagaaaa agaauggauu guucggcaac      720

aucauugccc ucgcacuggg cuuaacccca aauuuuaaau cuaacuucga ucugacugaa      780

gacgcuaagc uccaacuuag caaagacaca uaugaugacg aucuggacga guugcuaggg      840

cagaucggag aucaguacgc cgaccuguuc uccgcggcca agaaccucuc agaugcaauc      900

cugcuuagug acauucugcg uagcaauucu gaagugacca aggcucccuu guccgccuca      960

auggucaaac gcuaugauga gcaucaccag gaccucgcuc uguuaaagac gcuggugaga     1020

caacaguuuc cugaaaaaua cgccgagaua uucaaggaug acacuaaaaa cgguuaugca     1080

ggcuacguag ggaucggaau uaagcacagg aagcggacca caaaacuagc cacucaggag     1140

gaauuuuaua aguucaucaa accaauacuc gagaagaugg acggcgcuga ggaacugcuu     1200

gcgaaauuga aucgagauga ccugcuccgc aagcagagaa ccuuugauaa cgguagcauu     1260

ccccaucaaa uccaccugaa ggaacuucau gcaauccuga ggcggcagga ggaguucuac     1320

ccuuucuuga aagaaaaucg cgagaagauu gagaaaaucc ucacauucag gauuccauac     1380

uauguugggc cgcuggccag aggaaacagu cguuuugcuu gguuaacccg gaagucugaa     1440

gaggccauaa cgcccuggaa uuucgaagag guggucgaca aaggcgcauc cgcccagagc     1500

uuuaucgaac gaaugacuaa cuucgaugag caacugccua auaagaaagu gcuaccaaag     1560

cacucgcucc uguacgaaua uuuuacagug uacaacgagc uuaccaaggu caaauauguu     1620

acugagagaa ugaggaagcc cgaauuuuug ucuggcgagc agaaaaaggc uauuguggac     1680

cuucucuuca aaacaaaucg caagguaacc gugaagcagc ugaaagaaga uuacuuuaag     1740

aaaaucgagu gcuucgacuc cgucgaaauc auuggggugg aggaucgguu uaacgccuca     1800

uuaggaacgu aucacgaccu gcuuaagaua aucaaagaua aggacuucuu ggauaaugag     1860

gaaaacgagg acauucucga agacaucguu cugacccuaa cacuguuuga ggauagagaa     1920

augauugagg agaggcucaa gacuuacgca caucuguucg acgauaaagu gaugaagcag     1980

cuuaaacgcc gacacuacac cgguuggggc cgguugaguc guaagaugau caaugggaua     2040

agagacaaac aaagcggaaa gacaauccug gacuuuuuaa aguccgaugg cuucucuaac     2100

aggaacuuua ugcagcucau ucaugaugac agccugacuu ucaaagaaga gaucgaaaag     2160

gcgcaggucu caggucaggg ggauagucuu cacgagcaaa uugcugaccu ggccggcucc     2220

ccugcaauca aaaagggaau auugcagacc gugaaaauug uagaugagcu cguuaaggug     2280

augggccaua aaccagaaaa uaucgucauu gagauggccc gcgaaaacca gacaaccacu     2340

aaggggcugc agcaaagccg ggagagaaag aaaaggaucg aagagggaau aaaggagcua     2400

gaaucucaga uccugaaaga gaaucccgug gaaaacacgc agcuccagaa ugagaagcug     2460

uaucuuuacu auuugcaaaa cggucgagac auguacgucg aucaggaacu ggacauuaau     2520

cgcuuaucag acuaugaugu ggaccacauc guuccgcaga guuuuauuaa agaugacagc     2580

aucgauaaca aggugcucac acgguccgua gagaauagag gcaagucuga caacgucccu     2640

ucggaggaag ugguuaaaaa gaugaaaaau uacuggaggc aacugcuuaa cgcuaagcug     2700

auaacccagc guaaauucga uaaucuaacu aaggccgagc gcgggggacu cagcgaagca     2760

gacaaggcug gcuuuauuaa acggcagcug guggagacca gacagaucac aaagcacguc     2820

gccaggaucu uggauucccg aaugaacacc aaacgggaca agaaugauaa acccauucgc     2880

gaagugaagg uaaucacucu gaagucaaaa uuagugagug acuucagaaa ggacuuccaa     2940

cucuauaaag uuagggauau uaacaacuac caucacgcgc augacgccua ccugaaugcu     3000

gucgugggua cagcacuuau aaagaaauau ccaaagcugg agucugaguu uguguacggg     3060

gauuauaagg ucuacgacgu ucggaaaaug aucgccaaga gcgaacagga gauuggcaaa     3120

gcuacggcca agcgauucuu uuauuccaac aucaugaauu ucuuuaaaac cgaagugaag     3180

uuggcaaacg gagagauuag aaaacgcccu cucaucgaga cuaauggcga aacaggggag     3240

gugguaugga acaaggaaaa ggauuucgcg accgucagga aagugcuggc caugccccag     3300

guuaauauag ugaagaaaac agagguccag acuggagguu uuucaaagga aagcauccua     3360

ucuaaacgug aguccgcuaa gcugauucca cggaagaaag gcugggacac cagaaaguac     3420

gggggauucg gcagucccac gguggccuau agcauccuug uuguggcaaa aguagagaag     3480

gguaaagcua agaagcucaa aucugucaag gugcuggugg ggauuaccau cauggaaaaa     3540

ggcuccuacg agaaggaucc uauaggauuu uuggaagcca aaggcuacaa ggacauuaag     3600

aaagagcuga ucuucaaguu accaaaauau ucacucuuug aacuggagaa cgggaggcgc     3660

cgaaugcuug caagugccac agagcugcaa aaggcuaaug aauugguccu cccgcagcac     3720

cugguuagac uacuguacua uacucagaac aucagcgcga ccacaggauc gaauaaccuu     3780

ggcuacauug agcagcaccg ggaagaguuc aaagaaauau ucgagaagau cauugauuuu     3840

uccgagaaau auauccucaa gaauaaggug aacucuaauc ugaaaagcuc auucgacgaa     3900

caauuugccg uaagugauuc cauuuuguua ucuaacagcu ucgugucccu gcucaaguac     3960

acuucauuug gugcaagcgg gggauucacc uuucuggacc uugaugucaa acagggcagg     4020

cugcgcuauc agacggugac agagguuuug gacgcuaccc uaaucuacca aaguauaacu     4080

ggucuguacg aaacacguac cgaccucucu cagcugggcg gggauuga                  4128


<210>  3176
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3176
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggaugggc ccuggugaac       60

gaguccgaaa auaaggauga gcagucuuca auagucagau uggguguuag ggugaucccc      120

uuaacuguag acgaaaaagg caacuucgag aaggggaaag cuauuacgac caaugcagau      180

cggcaacagg ugcacgccgc gcgcaucaac cugcagcgau acaagcuacg uagagaaaau      240

cucagggacu gccugcagaa gcaaggaugg auuggcagug agccuaugug ugaggauggu      300

aaaagcucca cauuugaaac uuauaaacuu cgggcuaaag ccgcagucga ggaagugucu      360

cugcaugagu ucgcccgcgu uuuguuuaug cucaacaaga aaagagggua caagucgaau      420

aggaaggcua acgacaaaga ggaaggaaag cuguucgaug gcaugaccau cgccaaaaag      480

cuguaugagg aacaccucac accagccgag uacucauuac agcugcuuaa uaccggccgg      540

aaauuuacuc cggaauauua ccgcagcgac uugauagagg aguucgaaaa gaucuggaac      600

gagcagcaga aauauuaccc cgaaauucug acagaugagu uuaagcaaca gcuagaaggg      660

aaggcuaaaa guaauaccuc caaacucuuc cugggaaaau acgguaucua uucugccgac      720

acgaagggcc uugaucgaag acugcagccu uugaaaugga ggguggaggc gaugaagcag      780

caagucgaca aggaugugcu cgcauuugua auuagcgacc ugaaagggca gaucgccaac      840

acuuccggau uacugggcgc uauaucagac cggaguaagg agcucuacuu cagcaaacag      900

accauugguc aauaucuuug ggccucucug caggaaaauc cacauaucuc cauuaagaac      960

cagcccuuuu accgucagga uuauuuggac gaguucgaaa agaucuggga gacacaagca     1020

acuuaccaca aggaacuaac cucagagcug aagcaggaga uccgcgauau uauaaucuuu     1080

uaucagagac cucucaaaag caagaaaaau cugauugcug uuugcgaacu ugagcagagg     1140

aaggugaaag ucacagugga cgggaaggaa aaggagauca ccauaggacc aaaaauugcc     1200

cccaagaguu cuccuauguu ccaagaguuc cggaucuggc agaaccugaa caauaucuug     1260

cuccuggauc augaaacgaa cuccaaacga ccauuauacg aggaggaacg caaucugcua     1320

uacgaggaac ucagcgugaa ggagaaacug ucgaagacug aagcgcuuaa agucuugggc     1380

aagaagucua aacaguggga ccugaacuau aaggagcucg aaggcaauag aacacaggcc     1440

guucuguacg auuguuauaa caggauuauc gugauguccg ggcacgagga augcaauuuu     1500

aaaaagcuga aagcaucaga cauucggcac uacguaaaga ugauauucaa gaaccuggcu     1560

uuuaauaccg auaucuugug uuucgacagu agccucaaag gacaugagcu ggagcgccaa     1620

ccgaugugcc aguuauggca ccuucuauau uccuacgaag gugauaacuc uagaacuggc     1680

aaugacgccc uccugaggaa acuugagaca aguuuugggu uccccaagga auaugcugcc     1740

guguuggcag acgucauuuu ugaggauuca uacggaaacc ugagugugaa ggccaugcgu     1800

aauauccucc cucaucugca ggcuggcaaa gacuacuccc aggcguguga agaggcaggu     1860

uauaagcaca gccggcgauc uuuaaccaag gaggaacuga accaaaaagu uuacaaggag     1920

aaacuugaau ugcucccaaa gaacucacug cacaaucccg uggucgagaa aauccuaaac     1980

cagaugguga auguaauuaa cgccguuaua gaggaauaug ggaagccuga ugagaucaga     2040

auugaacugg cuagggaccu caagaucggc gccgcagagc gcgaaaauau gacgcggagu     2100

auuaaccagg gaaaugccga gaacaaaaga aucagggaga uacuggaaaa ggaguucgcu     2160

cuuagcuacu ugucccgcaa ugauaucauu aaauauaagc uguacgaaga guuaaaaucu     2220

aacggcuuua agacccucua uucggacaca uacaucagca aagauaagcu guucuccaag     2280

gacuuugaua uugaacauau cauaccaaaa gcccgacuuu ucgacgauuc acugaguaau     2340

aagacuauug aggcacggga cgugaacauc gcgaaaucua auaagaccgc uuuugauuuc     2400

auuuuggagc guuacgggga acaaggaguc aaaacauaua agacuaagcu ccugagacua     2460

cuggaggacg gugccaucag caacaaaaag uacaauaacc uccugaaaac cgaagcagac     2520

auacccuccg gcuucaucga uagggaccuu cgcaauacac aguauauugc caagaaggcu     2580

uacgagaucu ugucagaacu ggugaagauu gucaccccga cuacggggag cgugacaaac     2640

cgguuaagag aggauuggca gcucguugac gugaugaagg agcugaacuu ugaaaaauau     2700

cagaaacuug gacuggccga uguaaucgag gacagggaug gccgaaaggu caagcgcaua     2760

aaagacugga ccaagcggaa ugaucacaga caucacgcaa uggacgcucu aacuauugcc     2820

uucaccaagc cuucuuacau ccaauaucuc aacaaucuga acgcgagguc cgauaaaagu     2880

agcucuaucu acgguauuga agugaaggag uugcaucgug aggaagggaa auuucgcuuc     2940

aaugcacccc ugccaaucaa cgaguuucgg gcugaagcaa agagacacuu aaaauccguu     3000

cucgugucaa uuaaggccaa gaaaaagguc augacacaga augugaacaa aauaaagacc     3060

aaacacggca ucauuaagaa aguacagcug acuccuaggg gagagcuuca uaaugcuaca     3120

uacuauggca cgaagaagcg accccuggug cggaugguua aagucggggc cgcauuggac     3180

gaagcgacca ucaagcgcgu gugcagucca gccauuagag cugcccuccu gaaaaggcua     3240

aacgcauaca auggagaugc uaaggaggcc uucacugguc ggaacagcau cgacaaaaau     3300

cccauauauc uggacaucga gcagacaaaa cuugugccug uccucguuaa gaccauugaa     3360

uggggcaugu accacccaau ccgaaagaga auuacaccgg aucugaagau cgacaaagug     3420

auagauaagg ggauucgcga gaaauugaag gcaaggcugg ccgaauuuaa cggagacgcu     3480

caaaaggcgu ucucgaauuu agaugagaac cccaucuaua uggacgaagc caaacguauc     3540

acucucaagc ggguguccau ugagggcgua cugucugcaa uaccucuuca uacccugaaa     3600

gauaaggcug guaaaccagu caucgggagc gacggcaagc ccguguuugg agauuacguu     3660

cagacgucaa acaaucacca cauugccuuc uauaucgacg auaagggcaa cuugcaggac     3720

aauccuguga gcuuuuucga ggccgcugaa agaaaauccc aggggguccc agugauugac     3780

aggcgcuaca acugugauaa gggauggcau uuuguuuuca ccaugaaaca aaaugaguac     3840

uucguguuuc ccaacgaagc aacaggcuuc aucccgucug agguagaccu ccuggaugaa     3900

gccaauuauu gcauaaucag cccuaaccua uaccgagucc agaagcuguc caaagcguca     3960

gguagagacu auugguuucg gcaccaucuu gagacuaugc ucaaugauga ccugagguug     4020

aagaacagca ccuucaaacg cauucagagu uuaaaggggc uggaggauau cgugaaggug     4080

cguauuaauu cuaucggaaa aauagucgcu guuggcaagu acgacuga                  4128


<210>  3177
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3177
auggagacca aguucacaua cuaucugggc cucgacaugg ggacugauag ccuuggaugg       60

gccgugacgg acuccucuua ccacauccug agagcuaaag guaaggauuu guggggcguc      120

agguuauuuc ccgcagccga aaccucaguu gaccggcgca cauaucgagu gaaccguaga      180

aggcggcagc gcgagguagc gagaauuggg gcucugaaag aacuauucgc cgaugagaua      240

gaaaaggugg acccuggauu uuucgucagg cucgaugaga guaaauuuca uauggaggac      300

cggagcgaaa auaacaagca aaaguacggc cuguucgcag auaaacuauu uuccgacgcc      360

gaguauuaca agaaauaucc aacuaucuuc caucugcgca aggaauugcu cgagucugag      420

gaaccgcacg augugcgacu gguuuaccug gcucucuuaa auauguuuaa acacagaggu      480

cauuuccuga acucgucacu ucagauugag ggggaaaaua gcaacaucgc agagaguuac      540

cagauguuug uggacaccuu gaaggaugaa gaacuggacu uccccgagca ggucuccgga      600

gaaaggcuac aagagauuuu ucggucuagc gacucccgua agaaaaaggu ggaaaaucuc      660

cuggccgaga ugaaccuuag uaaaaagcag aaacgcgaaa uccaguucgu aaaucugaua      720

ugcggcuuga agggcaaacu cucagagcug uucccugaua aggaguauga cgaagaacag      780

aagaaaaucg cuuuuagcuu ccaagauucu gacuacgaag auaaggagaa cacaauucag      840

gaauuacugg acgaguccga gaugaauaug aucuaugccg uuaaagcggu gcacgauuca      900

gggguccuca gcggaauuau gaacggugaa agauaccuua guguggcaag gguggccucu      960

uaugagaagc acaaaaagga ccugcagauc uugaaggaua uacuuaaaaa guacgacacc     1020

aaagaauauu uugagauguu ccggaaugag uccgaaaaga gcgagaacua cucggcuuac     1080

gucggcucug uuaaauauca ugggacuuuu cgacgcggac uguccggcgg uaagcaggaa     1140

gaucucuaca agcacauuaa aaaggugcug gggaaauucc cacaagacga cgagggagua     1200

acauaugugc uugaagagau cgagaccgaa ucauuucugc ccaagcaguu gacggccagu     1260

aauggcguca uuccuaacca ggugcaugca agagagaugc ucgcuauccu gaaacacgcc     1320

gaauuauacc ugccauuccu acaagagaag gaugacacug gccucagcgu uuccgaaaag     1380

auccugaaac uuuuuauguu ccagauuccc uauuacgugg ggccuuuggg acaggaguau     1440

aaggauaaaa aggguuacaa cgucugggcg gagaggaaag aaccaggcaa gauauacccg     1500

uggaauuuug aggagaaaau cgacgugaag aagucugccg agaaauucau ugggcggaug     1560

guacgcagau guaccuaucu gagcggagaa caggcacucc ccaagcaauc acugcuuuac     1620

gagaaauuuc agguucugaa cgaguugaau aaccucagga uccguggcga aaagauaccu     1680

gugagucuga aacaggagau uuaucacgau uuauucggug acgggaagaa ggucuccaug     1740

acacggcuga cuaauuaccu acuccagaac ggcgugauca gcucugaaga gaaagauuca     1800

ugcaucagug gaauugacgg cggguuccau aauagccugu ccucugucgg aaaguuuaaa     1860

gguguguucg gcgauaccgu ucacacagac gagcaucgaa agaugaucga agauauuaua     1920

uuuuggggga ccguguucgg agacggcaaa aaguugcuua aggagaaagu agaagagguc     1980

uaugguaacc aacugacgga agcucagaag aaaagaaucu uuggguucaa guuuucgggc     2040

uggggaaggc ucagcaaagc cuuccugacu auggagggcg cuuccaagga uaauggggag     2100

auuaagggau uaauccaggc caugugggac acaaacugua aucugaugga acuuuuguca     2160

gagcgcuuua ccuacaaaga caagcucaac gaaaugguga cuacagcaga gaaaccacug     2220

ugcgaaugga agaucgagga ucuagacgag auguaucuga gugcccccgu uaaacggaug     2280

guguggcaga ccauuagagu cguggaugaa guacaacaga uaauggguug uccuccaaag     2340

aggaucuucg uggagaugac gcgcucugac ggcgaaaaag ggaagcgaac cguuagccgg     2400

cagaagaaac ucgucgaucu guacaaggcu cuuggaaaag agggcaagau uugguccgaa     2460

gaguugaaua acacacccga gggucaguuu cguaucaaaa agcuguacuu auauuaccuc     2520

caaaugggga agugcaugua uacuggcgaa ucaauugagc uggaaccgcu uaugaaugac     2580

aaccaccugu acgauaucga ccauauauau ccuagacacu ucaucaaaga ugacaacauu     2640

agcaauaacu uggugcucgu gaagaaagag aggaaugcgc acaagucuga uuccuuuccc     2700

aucgagccag aaauucgcaa caaaaugaug ccuuucugga agagucugcg gaagaaagga     2760

uuccuaaccg aggaaaagua caauagacug acaaggauga gcgcauuuac ugacgaggaa     2820

aaagccgcuu ucaucucucg acagcucguc gagaccuccc agggcacaaa ggccauaacc     2880

caaauuuuua aacaggcauu cgccgauuca gagaucguuu uuaguaaggc uagcguggug     2940

ucggccuucc gccagaaguu ugacuguuau aaaguacggu gcgucaacga cuaccaucac     3000

gcacaugaug cguaccugaa uauuguggcu gggaacacuu auagaguuaa guucacgucc     3060

aauccccuua acuuuaucaa agaagccaag aaaaauccaa acucuaggga gaaucguuac     3120

aacauggaca agacauucga augggaugug acccgcaaug gagagacugc augggucgcc     3180

cagagcaacg aaaaccccgg caccauacaa uuggugaaga aauaucugaa uaagaacuca     3240

ccuuuaauca caaaaauggu uuacgagaag cacgguggga uuacccagaa agcuacuauc     3300

uggagugcca agaaagcaga guccggaaau acagacgcuu auauuccagu gaagaugaac     3360

gauccgcggc ucgccgacgu aacgaaguac ggcgguguca ccacuaucgc gguggccggc     3420

uauacacugc uugaauacaa agcuggggga aagaccguga gaucucugga ggcacuaccc     3480

auauaccucg gcgggagcaa aaaucugaca gaggagcaga uggucgaaua uuuuuccaac     3540

auuuugcagc aagagaauaa ggagaaaguu gugucagaug uaaucaucag gaagaagaug     3600

auuccuagcg gaagucugau caaauacaac ggcuucuauu acuauuuagc cgguaagacu     3660

gggaaucgaa uugugcucau aucugcuguc cagcuguguc uuccaaugga caccaugcug     3720

uacaucaaaa agauugaaaa agccugcucc acgggaaacu ucgaggaaaa ggauaagaau     3780

ggcaaacagg uguugagcaa gcagcaaaac gaguuuguuu augagcucau cauugaaaaa     3840

uaccagcagu cgaucuucaa gaaaaaggug ggugcaauag gcgacaccau cauugagaag     3900

cgggaaaaau uugcggagau cucacuggag gaacaaugua agguccuaau gcagauuuuc     3960

cugaauuuuc agucugggau cggaguggau cuuacaagug uugacggcuc caacaaaagc     4020

ggggugacuu accucaauaa gaaaauaaug aacgcccagg agcugaaguu gauugaccaa     4080

ucugucaccg gaguauucag ccgcgaaauc gaucuguuaa caaucuga                  4128


<210>  3178
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3178
augagcaaca aguccuacuc uaucggccug gacauuggga ccaauucagu gggaugggcc       60

gucauaacag augacuauaa aguucccagu aagaaaauga aggugcucgg uaacacugau      120

aaacacuuca ucaagaagaa ucuuauuggc gcucuguugu uugacgaggg gacgaccgca      180

gaagauagaa gguuaaaacg gacagcccgc cgacguuaca cuagaaggaa gaaccggcug      240

cgcuaucuac aggagaucuu cagcgaagag auguccaaag uagacucuuc guuuuuccau      300

agacucgaug acucauuucu ggugccugaa gauaagaggg gaagcaaaua cccaauuuuc      360

gcgacccuug cugaggagaa ggaauaucac aagaaauuuc cgacaaucua ccaucugcgg      420

aagcacuugg ccgacaguaa agagaagacc gaucuccgcc ugauauaucu ggcacucgcc      480

cauaugauca aauaccgagg ccacuucuua uacgaagagu ccuuugacau uaagaauaac      540

gauauccaaa agauuuucuc ugaguuuauc agcauauaug acaauacuuu cgaagagucc      600

ucacugauug gucagaacgc ucaggucgaa gcaaucuuua cagacaagau uaguaagagc      660

gccaaaagag agagggugcu uaaguuguuc cccgaucaga aaucuaccgg gcuguucucc      720

gaauuucuaa agcucauccu gggaaaucaa gcugacuuca aaaagcacuu ugaucuugag      780

gagaaggccc cucugcaguu cucaaaagac acguacgaug aagacuugga gaaccuccug      840

ggccagaucc gggaugacuu ugcggauuua uuccugguug caaagaaacu cuaugacgcc      900

auucuucuga gcggcauauu gacugugaag gaucuaagua ccaaagcucc acugucugcc      960

uccaugaucg aacauuacga gaaucaccag aaggaccucg cagcucugaa gcaauuuauu     1020

cagaacaauc uucaggaaaa auaugacgag gucuucagcg aucagucgaa ggacggguac     1080

gccggauaua ucaacgguaa aacaacucaa gaggcguuuu acaaguacau aaagaaucug     1140

uugucuaagu ucgaaggcgc cgauuauuuu cucgacaaga uugagcguga agauuuccug     1200

cgcaaacaga gaaccuuuga caacgggucc aucccccauc agauccacuu acaagagaug     1260

aacgcaauuc ugcacaggca gggagaacau uacccuuucc uaaaggagaa uaaagagaag     1320

aucgagaaaa uucucauauu ccggauccca uauuacgugg gcccccuggc ucgagguaac     1380

cgcgauuuug ccuggcuuac aagaaauuca gacgaagcua uuaggccuug gaacuucgaa     1440

gagaucguag auaaggccag uagcgcagag gacuuuauca auaaaaugac caacuaugau     1500

uuguaccugc cagaagagaa gguucucccg aagcacuccc ugcuuuauga aacguucgcc     1560

guguacaaug agcugacuaa agucaaguuu auugcugaag gguugacacg guaccaguuc     1620

cucgacaaaa agcagaaaaa ggauauauuu uauaccuucu uuaaggcgga ggacaaacgc     1680

aaggugacug agaaagacau cauucauuac cugcacacag uggauggaua ugacggcauc     1740

gaauuagagg gcauugaaaa gcaauucaac gcaucucuga gcaccuacca ugaucuacuc     1800

aaaaucauaa aggacaagga guuuauggau gacgauaaaa augaagccau ccuggagaac     1860

auuguccaca cgcuuaccau cuucgaggac agagaaauga uuaagcagag guuggcucag     1920

uauaauucac uguuugauga gaaaguuauc aaggcccuca cacgucggca cuacacuggg     1980

uggggaaaac ugagugcaaa guuaauaaac gguauucgag acaagaaauc cggcaagacc     2040

auccuggauu aucuuauuga cgauggggaa aucaauagaa acuucaugca guugauacau     2100

gacgacggac ucagcuucaa agagaucauu caaaaggccc agguguuugg caaaacaaau     2160

gauguaaaac agguggucca cgaacugccc gguucuccug cuaucaaaaa ggggauucua     2220

cagucaauca agauagugga cgagcugguu aaagugaugg gccaugcccc agagaguauu     2280

gucaucgaaa uggcaaggga gaaccaaacu accgcgcgcg gaaagaaaaa cagccagcag     2340

cgguacaaga gaaucgaaga uucccucaaa aaucuggcuu cuggccuuga cucgaacauu     2400

uugaaggaga aucccacaga uaacgugcag cugcaaaaug acagguuauu ccucuacuau     2460

cugcagaacg ggcgcgauau guacaccgga aagccucuug agaucaauca gcugagcaac     2520

uaugacauug aucacauaau cccacaagcc uuuauuaaag acgauuccuu ggacaaucga     2580

guacucacuu caaguaagga caaccggggu aaaucugaua auguucccag ccuggaagug     2640

gucgagaaga ugaaagcauu cuggcagcag cuacuggacu ccaagcucau cucagaacgu     2700

aaguuuaaca aucugacgaa agccgagaga gaaaggggcg ggcuuaacga guuggauaag     2760

gugggauuca uuaaacgcca gcuggucgag acacggcaaa ucaccaagca cguggcucag     2820

auauuagaca cuagauuuaa uaaagaaguu accgagaagg auaagaaaaa caggaacgug     2880

aagaucauua cacucaaaag caaucuggua ucuaacuucc gaaaggaauu uggccuuuac     2940

aaaguccgcg agaucaauga cuaucaucac gcccaugaug cauaccugaa cgcugugguu     3000

gccaaggcga uucuaaagaa auauccgaag cucgaaccug aguucgugua cggugaguac     3060

cagaaauaug accugaagcg guacaucucc agaaguaaag aucccaagga cauagaaaaa     3120

gccaccgaga aguacuuuuu cuauagcaau uugcugaacu ucuuuaagga agagguccag     3180

uaugcugaug ggacuauugc aaaaagggaa aauaucgagu acucuaagga cacaggcgag     3240

aucgccugga acaaagaaaa ggauuucgcu acggugaaaa agguauuauc ccucccacaa     3300

gugaauauug uuaagaaacg ugagguccag accggaggcu uuucaaagga aaguauacug     3360

ccuaaaggga acagcgacaa acuuaucccc cgcaaaacua aggacauucu gugggagaca     3420

accaaguaug gagguuucga uucgccagug aucgccuacu ccauuuugcu caucgcagac     3480

auagagaaag gcaagucuaa agaacugaag acagugaaaa cucuagucgg gaucaccauu     3540

auggagaagg cgacguuuga agagaauccc aucaccuucc uggaaaacaa gggauaccac     3600

aauguucgga aagagaacau ucuuugccuc ccuaaguaua gccuguuuga guuggaaaau     3660

ggcagaaggc gacuguuagc cucagcuaaa gagcuccaga aggguaacga aaucgugcug     3720

ccaguguacc uuacaacucu guuguaucau aguaaaaacc uccacaagcu ggaugagccg     3780

gggcaccuag aauacauaca gaaacaucgg aaugaguuca aggaccugcu uaaccucgua     3840

uccgaguuuu cucaaaagua uauucuggcc gaugcaaauu uggagaaaau ccagaacuua     3900

uacgcugaca augaacaggc cgauaucgaa auucuggcaa acagcuucau caaucuccug     3960

accuuuacaa cucuuggcgc ccccgcugcg uucaaguuuu ucggaaaaga cauagaucgc     4020

aagagauaua ccacggucuc cgagauucug aacgccacau ugauccacca aucaauuacc     4080

ggccuauacg aaacuaggau cgaccugagc aaacucgggg aggauuga                  4128


<210>  3179
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3179
auggaaaaga gcuacuccau cggccuggac auugggacca acucuguggg augggccguc       60

auaacagaug acuauaaagu ucccgcuaag aaaaugaagg ugcucgguaa uacugauaaa      120

aaguacauca agaaaaaccu ucugggcgca uuguuauucg acucagggga aacggccgag      180

guaaccagac ugaagaggac agcgcggcgc cgauauacuc guaggaaaaa uagacuacgg      240

uaccuccagg aaauuuuugc uaaggagaug accaaagugg augaaaguuu cuuucaacgc      300

cuggaggaga gcuuccuuac agacgaugac aagaccuuug auucccaccc uaucuucgga      360

aacaaggccg aagaggacgc auaucaucag aaauuuccaa cuauuuacca ccugagaaag      420

uauuuggccg auucucagga aaaagcugac cucaggcugg ucuaccuggc acucgcccau      480

augaucaagu accggggcca cuucuuaaua gagggugagc ugaaugcuga aaacacagau      540

gugcagaaac uuuuuaaugu uuucguggag accuaugaca agaucgucga cgaaucgcau      600

uugucagaga uugaagugga ugccagcagu auccugacgg agaaaguauc caagucucgc      660

cgacuagaga accucauuaa gcaauacccg acugagaaaa agaauacccu guuugggaac      720

cuuaucgcgc uggcauuggg acuccagccc aauuucaaaa caaacuuuaa gcugagcgag      780

gacgccaaau uacaguucuc caaggauacu uaugaagagg accuggagga gcuccuuggc      840

aagguuggcg augacuacgc ugaucuguuc auaucagcca aaaauuugua ugacgcaauu      900

cuacugagug ggauccucac cguggaugac aacagcacaa aggcuccucu gucugccucc      960

augauuaaaa gauacgucga gcaccacgaa gaucuugaga agcugaaaga auuuaucaag     1020

aucaauaagu ugaaacucua ucaugacauu uucaaggaca aaaccaagaa cggauacgcg     1080

gguuacauag auaacggcgu gaaacaggac gaguuuuaua aguaccugaa gacgaucuua     1140

acuaaaauug augacucaga uuauuuccug gacaagaucg aaagggauga cuuucuacgg     1200

aaacaacgua cauucgauaa ugggagcaua ccacaccaga uucaucucca ggagaugcac     1260

aguauccugc gcagacaggg agacuacuau cccuuucuua aggagaacca agccaaaauc     1320

gaaaagauuu ugaccuucag gaucccuuac uaugugggcc cacuggcacg gaaagauucu     1380

cgauuugcuu gggccaauua ccacuccgac gagcccauua cuccuuggaa cuucgaugaa     1440

gucguugaca aggaaaagag cgcugagaaa uuuauaacac gcaugacccu caaugaccug     1500

uaccuuccag aagagaaggu gcugccgaaa cauucgcacg uauaugaaac guucaccgug     1560

uacaacgagu ugacaaagau caaauauguc aaugaacagg gugaguccuu uuucuucgau     1620

gccaacauga agcaggagau uuuugaccau guguucaagg aaaauagaaa aguuacuaag     1680

gcaaaacucc ugucuuacuu aaacaaugag uuugaagagu ucaggaucaa cgaucugauc     1740

gggcuagaca aggauucaaa aaguuuuaau gccagccucg gaaccuauca cgaccugaag     1800

aagauucuug auaaauccuu cuuggacgau aagacaaacg gccaaauaau cgaagacauu     1860

gugcugacuc ucacccuguu ugaggaucgg gacaugaucc augagcgcuu acagaaauac     1920

ucugauuucu uuacaagcca gcagcugaag aaacuugaaa gaaggcacua uaccggcugg     1980

gggcguuugu cauacaagcu cauuaaugga auccggaaca aggagaacaa uaaaacuaua     2040

cuggacuucc uaaucgacga uggucacgcu aaccgaaauu uuaugcaacu gauuaacgac     2100

gaaagucucu ccuucaagac gaucauucag gaggcgcagg ucgugggcga uguagacgau     2160

aucgaagcag uugugcauga ccugcccggg agcccugcca uuaaaaaggg gauacuucag     2220

ucugucaaaa ucguggauga guuggucaag gugaugggcg acaauccaga uaacauuguu     2280

aucgagaugg cuagagaaaa ucaaacaacc gguuacggga ggaacaaauc aaaucagcgc     2340

cugaagcggu uacaggacag ucucaaggag uuuggcagcg auauacuguc caaaaagaaa     2400

cccucuuaug uggacucgaa gguagaaaac agccaccuuc agaaugacag acuguucuug     2460

uacuauaucc aaaacggaaa agauauguac acuggcgagg agcucgacau ugauaggcug     2520

uccgacuaug auaucgacca uauuaucccu caggccuuca uaaaggauaa uucaauugac     2580

aacaaggucc uaaccaguuc ugccaaaaau cgcggaaaga gcgaugacgu gccauccauc     2640

gaaaucguuc gaaaccggcg uucauacugg uauaaacugu acaagagcgg acucauuucu     2700

aaaagaaagu uugauaaccu gacaaaggca gagaggggug gccuuaccga agcugacaaa     2760

gccggguuca ucaagcgcca guugguggag acucggcaga uuacaaaaca cgucgcacaa     2820

auacuggaug cgagauuuaa uacgaagagg gacgaaaacg acaaagugau ccgagaugua     2880

aaggugauua ccuuaaaguc caaucucguu agucaguucc gcaaagaguu uaaguucuac     2940

aaaguccggg agaucaacga cuaucaccau gcucacgaug ccuaccugaa ugcaguggug     3000

ggaacugccc uucugaagaa auaucccaag cuaacaccgg aauuugucua cggcgaguau     3060

aagaaauacg acguuagaaa gcucauugcu aaaagcucug augacuauuc cgaaaugggu     3120

aaggccaccg caaaauacuu cuuuuacuca aaccugauga auuucuuuaa gacagaggug     3180

aaauaugcug augggagggu guucgaacgu ccugacaucg agacuaacgc cgauggcgag     3240

guagucugga auaagcagaa ggacuucgau auagugcgca aaguuuugag uuacccccaa     3300

gugaacaucg ucaagaaagu ggaagcgcag accggaggcu uuagcaagga gucgauucug     3360

uccaaagggg acucugauaa guuaauccca cggaagacga aaaagguuua uuggaauacc     3420

aaaaaguacg gagguuucga cagcccuaca guggccuauu caguacucgu cguggcugac     3480

auugaaaaag gcaaggcaaa gaaacugaag acugugaaag agcuugucgg gaucaguaua     3540

auggaaagau ccuuuuucga ggagaacccc guuucuuuuc uggaaaagaa aggauaccau     3600

aaugugcagg aggauaaguu gauuaagcuc ccaaaauaua gccuguucga auuugagggc     3660

gguaggcgac ggcuacuggc cuccgcuacc gagcuucaga aggggaacga aguaaugcuc     3720

cccgcccacc ugguggaguu gcuguaccau gcacaccgca ucgacucauu caauagcaca     3780

gaacacuuaa aauacgucag ugagcauaag aaagaauuug agaaggugcu cucuugcguu     3840

gagaacuucu ccaaccugua uguggauguc gagaaaaauc uuagcaaggu gagagcggcc     3900

gcugaaucga ugacuaacuu uucacuggaa gagaucucug ccaguuucau uaauuugcuc     3960

acccugacgg cacuaggcgc uccugccgac uuuaacuucc ugggagaaaa gauaccaagg     4020

aaacgguaca cauccaccaa ggagugucuu agcgcaacuc ucauccacca aucuguuaca     4080

ggccuguaug agacccgaau ugauuugagc aaauuagggg aagaguga                  4128


<210>  3180
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3180
augaccaaga ucaaagacga uuacauugug ggccuggaca uagggacaga uagcugcgga       60

ugggucgcca ugaacuccaa uaacgacauc cucaaacuuc aggguaaaac ugcuauuggc      120

ucuagacugu ucgaaggggg aaagucagca gccgaaaggc gguuguuucg cacgacccac      180

cgacguauca aaagaaggcg guggcgcuua aagcugcuag aggaguucuu ugaucccuau      240

auggcggagg uugacccuua cuucuuugcu agacucaagg aaaguggccu gagcccacuu      300

gauaaaagga agacaguguc cucuauugua uucccgacuu cggccgagga caaaaaguuu      360

uaugaugacu accccaccau cuaucaucug cgguacaaau ugaugacaga ggaugaaaag      420

uucgaccucc gcgaggugua ucuggcaaua caccauauca uuaaguaccg agguaauuuu      480

cuguacaaca ccucagucaa agauuucaag gccagcaaaa ucgacgugaa gaguuccauu      540

gaaaaacuca augaguuaua ugagaaccug gggcuugacu ugaauguuga auuuaacauc      600

ucuaauacug cugagauaga aaaggugcug aaagauaagc aaauuuucaa gagagacaaa      660

gucaagaaaa ucgcagagcu auuugccauu aagacagaua acaaagaaca gagcaagagg      720

aucaaggaca ucuccaaaca gguggcuaau gccguacucg gauacaagac ccgguucgau      780

acgauugcgc ugaaagagau aucaaaggac gagcuuagug auuggaacuu caaacugagc      840

gacaucgaug cagacucuaa guuugaagcc uugaugggca aucucgauga gaacgaacag      900

gcuauucugu uaacuaucaa ggagcuguuc aaugaaguua cccucaacgg cauaguggag      960

gacgggaaca cacuuuccga gucaaugauu aauaaauaua acgaccaccg ugaugaccug     1020

aaguugcuaa aagaagucau cgagaaucau aucgaucgca agaaagccaa ggaacuggca     1080

cucgcuuacg accuguaugu gaacaauaga cacggacaac uucugcaggc caagaaaaag     1140

uuggguaaaa uuaagccuag gagcaaagag gauuuuuaca agguggucaa caaaaaucuc     1200

gacgauaguc gggcgucuaa ggaaaucaag aaaaagauug agcuggacuc cuucaugcca     1260

aaacagcgaa cuaacgccaa uggcguuaua cccuaucagu uacaacagcu ggagcuagau     1320

aagaucauug aaaaccagag caaauacuac ccuuuucuca aggagaucaa uccagugucg     1380

ucucaccuga aggaagcacc cuauaaacuu gacgaguuga uccgcuucag aguaccuuac     1440

uauguggggc cacugauuuc cccgaacgag ucaaccaagg auauacagac uaaaaagaau     1500

caaaacuuug cuuggaugau caggaaagaa gagggacgga uuacccccug gaauuucgac     1560

cagaaggucg aucgcaucga aagugccaac aaguuuauua aaagaaugac gacuaaggac     1620

acauaccucu ucggcgagga cgugcugccu gcuaacagcc uucuguauca gaaauuuacc     1680

guuuugaaug aacucaacaa uaucaggaua aacgguaagc guaucuccgu ggaucugaaa     1740

caagagauuu acgagaauuu auucaagaag cauacuacag ucaccgugaa aaagcuggaa     1800

aacuaucuaa aagagaauca caaccucgua aagguugaaa ucaaagggcu ggccgacgag     1860

aagaaauuua auucuggacu uacgaccuac aaccgguuca agaauuugaa cauuuucgau     1920

aaucagaucg acgaucugaa guaccgaaac gacuuugaaa agauaauuga guggagcaca     1980

aucuucgagg auaagucaau uuauaaagaa aagcucagaa guaucgacug gcugaaugag     2040

aaacagauaa acgcauuauc caacaucagg cugcagggcu ggggccgccu uagcaagaag     2100

uugcucgccc aacugcauga ucacaauggg cagacuauua ucgaacagcu augggacucu     2160

cagaacaauu uuaugcaaau ugugacccag gcugauuuca aagacgcgau cgcaaaggcc     2220

aaccagaauc ugcucgucgc uacaucagug gaggauauac ugaacaaugc cuacacaagu     2280

ccagcaaaca aaaaggccau ucggcagguc aucaaagugg uugacgacau cgugaaggcu     2340

gccagcggaa agguacccaa acaaauugca aucgaauuua ccagagaugc ggacgagaau     2400

ccuaagaggu cccagacacg cgguucuaaa cuucagaagg ucuauaaaga uuugucgacc     2460

gagcuggcua gcaagacuau ugccgaagag uuaaacgaag caauaaagga caaaaagcuc     2520

gugcaggaua aauacuaucu guacuucaug caacuuggcc gagacgccua uacgggggag     2580

ccaaucaaua uugaugagau ccagaaguac gacauugauc acauccugcc ccaguccuuu     2640

auaaaagacg augcuuugga caaccggguu cucgugucac gugccgucaa uaacggaaag     2700

agugacaaug ugccgguaaa gcuguucggc aacgaaaugg cagcuaaccu agguaugaca     2760

aucagaaaaa ugugggagga auggaagaau auugggcuga ucucuaaaac caaguacaac     2820

aaucuccuga cugauccuga ccauauuaac aaauauaaga gcgccggcuu uaucaggcgc     2880

caacuugugg agaccuccca gauaauuaaa uugguuucaa caauccugca gagccgguac     2940

cccaauaccg aaaucauuac ugucaaggcg aaguauaacc acuacuuaag agagaaauuc     3000

gaucucuaua agucuaggga ggugaaugac uaccaucacg ccauagaugc uuaucugucc     3060

gcaaucugug gaaaccuucu guaccagaau uacccaaacc uacgacccuu uuucguguau     3120

ggccaauaca aaaaguuuag uagcgacccu gauaaagaaa aggccauuuu caauaagaca     3180

cgcaaauucu cuuuuaucuc ccagcuccug aagaacaaau cagagaauag uaaggaaauu     3240

gcuaaaaagu ugaagcgggc cuaucaguuc aaauacaugc uggucagcag agagacggaa     3300

accagggacc aggagauguu uaagaugacu guuuauccac guuucucgca ugauacagug     3360

aaagcacccc gcaacuuaau cccuaagaaa auggggaugu ccccagacau auacggaggu     3420

uauaccaaua acucugaugc guacauggug aucguacgga uugacaagaa gaaaggcaca     3480

gaguacaaga uccucgggau uccgacuaga gaacugguca aucuuaaaaa ggccgagaaa     3540

gaagaucacu auaagagcua ccugaaagag aucuugaccc ccaggauacu cuauaacaag     3600

aacggaaagc gagacaaaaa gauuacguca uuugaaaucg ugaaaaguaa gaucccuuac     3660

aaacaaguua uucaggacgg cgauaagaag uucaugcugg guuccucuac cuauguguac     3720

aaugcuaaac agcuaacacu gagcacugag uccaugaagg ccaucaccaa caauuuugac     3780

aaagauucag acgagaacga ugcacuuaua aaggcuuaug acgaaauucu cgauaaaguc     3840

gacaaguacc ugccauuguu cgauaucaau aaguuucggg agaaacugca cagcgggcgc     3900

gaaaaguuca uuaaauuaag ucucgaggac aagaaagaua caauccugaa ggugcuugag     3960

ggccugcaug acaacgccgu uaugacuaag auacccacca uuggauuguc uacgccucuc     4020

ggcuuuaugc aguucccaaa uggggugauc cuguccgaaa acgcaaaacu aaucuaccaa     4080

agccccacag gacuguucaa gaaaucggua aagauuucag aucuuuga                  4128


<210>  3181
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3181
augagcaacu ucgacaauca caaggauucc aucugcaaac auaagggccu gaaaaagggg       60

aacuacuaua uuggacucga cauagguacc aauucugugg gcugggccgc uacaaacgag      120

caguacgaac uucugaaauu uaagucacac aagauguggg ggaguagauu guucgcagag      180

gcccaaacug cggucgauac gaggaugaaa cggagcgcuc gccgacguuu acagagaagg      240

aaguuucggc ugauccuacu cgaaucccug uucucuaaag agauuaaugc cguugacgaa      300

accuuuuuca ugcgccuuca ugagucgaac uaucacgugg aggauaagac aaaucuggua      360

aaauacacuu uguuuaucga caagaacuau accgaugugg acuacuauaa ggaguucccc      420

acaauuuacc aucucagaca ccaccugaug accgaaggaa cuaaagauau caggcugcuc      480

uuuuuagcaa uacaucacau ccugaaguac cggggcaauu uccuuuaugg uaaaaaggaa      540

uuuucagucg acggggagau guacagcgug uugcgcagua cacuggagga acuaggauuc      600

ggcuccuaug uugaggauga aaacgugcuc cagucuauug ccaaucuggu ccuugaccga      660

ggcaucgcua gaagcgagaa agugaagcag auugaaccuc ugaucgcaca agggaaaaag      720

uuguccucag uagagaagaa caggcucaaa gccugggcuu accugauaau gggauuaaag      780

gccaaucugg uuaaccucuu uaccgaugag gacguggaag ucgugcuucc agaggaaaaa      840

gaguucaaga guauuaaucu gcaugaaggu aucuaugagg acgugcggga ugcguacgag      900

gacgcauggg gcgaucguuu cgccauuguc gaccagugua aaguuuugca cgauaucauc      960

gugauuagcu cuauaaucaa gccggggcag ucccuaucag aagcuaagau ugagagcuau     1020

aaccagcaca aagaagaccu gguacuccug aagagugugc uuaaagagga uaagaaacug     1080

uacgacgaua uguuugucgu ggacaucgau aagggaacga acuacguuaa guauauaaaa     1140

caaggcguga augguaacac uucuaccaca caggaagccu ucuacaagua uguccgcaaa     1200

gugguauccg uuuugcccga gacugagaau accagaugca uugugcagaa gcucgaagag     1260

gggacauuuc ugccuuuaca gaggaucagc aaaaacggag ucgugccaua ccaacugcau     1320

aaggaggagc uaguccggau ccucgaaaag gcaaaacuga auuuccccuu ucuuaacgaa     1380

guggaggacg gcuugaccgu ugcugacaag auuaucucga uucuggaguu caaaauaccu     1440

uauuacgugg gcccacucaa ucccacgcac ucuaucgaaa acgggggauu ugccugggua     1500

guccgaaagg aagcggguca ggugcugccu uggaauuucg agcagaaagu ugaugugcaa     1560

gccuccgcag ucaaguuuau ugagaaccuu acuaauaaau guacauaucu gaucgaccag     1620

gauguguugc caaaguacuc acuccuguac gcugaguuca guuuacugaa cgaacuaaau     1680

aacauccgcg uaaaugacaa gccgcucccc gccgauguga aacaggcuuu uauuagagac     1740

cauuucauaa gccugcagga uuccaagacc gccacuaaaa agguugucgc aaaauaucuu     1800

guggacaaca acuacguguc ucaaccucca aucauuacag gcauggauca gcagaucgcc     1860

accaaguuga cgagccacag ggacaugacc cggauucugg gggaacauuu ugauuaugcu     1920

auggcggagg acaucauacg cuacaucaca auuuucggag aaucaaagga uaugcucaga     1980

cagguccuga aaaauuauua cggcgacagg uuaacuaccg agcaaaucaa gaaacugggu     2040

aaacuuacau auacugggug gggccguuug aguaagaagu uccucuccga cauuugggga     2100

augaagaaaa acgaugacga ugcagaggcc ggcaccauca uagaacacau gcgggagggg     2160

caggaaaauc ugaugcagcu acugucuagc ucauacaguu uuauugacgu ugugcaggag     2220

uacgugacaa acguacucgg agcuaaggag aaaaccgccu ucgaaaugcu ggaggaucuu     2280

agcuuguccc ccauggucaa gcgaucugug uggcaaacuc ugagaaucuu agacgaaauu     2340

guuucgauca ggaaagagcu cccuaagaag auauuugugg aagucgcacg cacgaauaaa     2400

gccgauaaga aacggacaca gagcagaaag gcucugcuug aggagcugua ugaacagauc     2460

aaagacgagg augugcacgu uuugcagcaa gaacucgagu ccaaggaaga cucagcccug     2520

aacaguaaaa agcuauaccu guauuucacc cagaugggua ggugcaugua cucuggccgc     2580

cgaauugaua uccaugagcu cuuuacugac accuaugaua uugaccacau cuacccacgg     2640

agccugacaa aggacgauuc cuggcauaau auagugcuug uagagaaaca guugaacgca     2700

gaaaagaccg accguuuccc cauucuguca gcgauccaaa gcaaacagaa gggguuuugg     2760

aaauuacucu gggauagagg auucaucucu gagaagaagu augacaggcu gguccgcacu     2820

acagaucuua cggacgagga acugaaucag uuuauuaacc ggcagaucgu guccaccaau     2880

caaaguguga aagcugucac uacacuacuc caccagcugu acccggaaac cgagguugug     2940

uucagcaagg ccgagaacau uucugauuuu agaagggacu acggcuucgu aaaagugcga     3000

uccauaaauc accaucacca ugcaaaggau gccuauuuga acaucgucgu ggguaauguu     3060

uaccacgaaa aauuuacaaa gaacuuccug ucauucgcua agcagaaagg gacucaucgc     3120

accuauaacu uaacgaagau guuugaguac gaaauuaaac ucaccgagga aaguaagaaa     3180

gugaucuggg acccuaauau ucacauggau aucgucaaga agaugaugaa aagcaacgac     3240

gugcggguua cacagagagc cgugccccaa aagggcggac ugucggacga gacuauauau     3300

aaaaagggca ucgcaaaacg uggggucuac cuuccacuga aguccucuga ugagagggua     3360

cgcgacgugg aaaaguaugg aggucggacc agcauuacaa ucucagagua cauuaucuug     3420

aauauaagag auaccaguga ccaggauagg auugaaaucu ucucccuccc ucuguacauc     3480

gcuggcaaaa aggacauuca caaagccaua gaggaggcgu aucuaucuac uuguaagaaa     3540

cccgccagcg uuguggauau ccagauucug uaccgagggc uuuauaucgg auccaagguc     3600

aacauuaaug gcuuuacgua cuauaugggu gggaaaacag gcucaaacgu gagcguagac     3660

agugcugugg augucguuau ggacaucacc ucuuccgugu acauaaagcu ccuggaaaag     3720

guggcaauca aaaagauuga gaauagcaaa acuuuccgga ucgaaaacau uaagacaucg     3780

uauaaucaua aagaggucac cgugucaaag gaacagaaca ucgccuugua ccaagagcug     3840

uuaaagaaac ucgauacucc acuguuuucu aagaugaaag gaaauaaacu ugaggaguuc     3900

cgcaccguug gcuuugacac auucauagcu cugaguuugg aagaucaggc cuccguacuc     3960

cuggaagugc uaaacguccu gacgaauuuu aagaccaaga gagacauuac uaagaucggg     4020

augacaagcu cuaggagcac ccuuggaauc aacauuucca auacagagca guucucagug     4080

aucacuacca guauaacggg ccucuacgaa caagagauua aaaucaagug a              4131


<210>  3182
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3182
augaccacaa agaacagaga gaaaaauaac aaguuccaca aagccagccc ccuguacacu       60

cucggccuug acaucgggac gaccuccgug ggauuuacag cuauugauga agcacagagg      120

ccuaugcggg ccaaggguaa aacugucaua ggcguucgcc uguuccaaga ggggcagucu      180

gcggugggac gaaagaauuu ucguaccaca agaaggcggu ugaagcgcag aaggcggcgc      240

uuaaaccugc uaaaacaguu cuuuucagcu ccacuccgaa agaucgaccc gaccuucuuu      300

aaaagacuga aggccaguag ccuuuccccc gcagauaaaa ggcgguaucu ggccccuggc      360

uuguucccaa auggugggga cauuaaguuu uaccagaagu aucccacuau cuaccaucuc      420

cgucuggcuc ugaugacaga aaaacgcccu uucgaucuca gagagguaua uuuagcaauu      480

caccauaucg ugaaguacag gggaaacuuu cugaauccac uucccgucuc ugccuuccgg      540

ggccgaaacc aacgcuuuga agaccaguuc ucggaguuga aucagcugua uaaaucacua      600

gagggcgaaa gcccuuucca guuugaugag aagauauuca gugaacucga caaacaacug      660

cuugugaacg augggagaaa gaucgacagg cagaaacagc uggcuaagcg gguuuuuauu      720

aagaccaaug agaaacgcau cgauucccac caucagaaga uugccucugc guucagcaaa      780

gcaguguuug gauacaaguu cagaccagac gucuugcuca acuccgaucc gaaugaguca      840

aucgacgaau uuauaaaauu cggugacgag gauuuugacg auaagcuggu gauuuuaagg      900

gaaagucuga acgccacgca aaaggagauc cucgcuguaa cucagcguau uuacagccag      960

aucauccuua augaaauugu ucccucucgg gagacccugu ccgaggccau ggugucacga     1020

uaucaaaaac accacagcga cuugcagcua cugacagaag caugcaagaa aaugacuaag     1080

gcuaaagccg cgagaauaca ggccgcauac accgauuaua ucggcaaggg ggacggacau     1140

aagcucgucc acgaucaguu cguggcucug auuaaaaaga accuugaccc uagugaggaa     1200

ggcagggauc uggccgagcg cuuggcucaa gacgauuuua ugccacucca gcggacaccc     1260

cugaacagag ugaucccuca ucaguuacag caagaagaac uggacaggau aauugagaau     1320

cagggugaau acuauccauu ccuaaaaacc cucaacccca aucgcucucg agccgggguc     1380

gcaaacuacc agcuggacga gcuuguuucc uuucggaucc cguauuacgu gggaccuuug     1440

aucacggcca cugaucagcu gcacacaagc ggcgcuaagu ucgcguggau gacccguaga     1500

gcagccggcg aaauuacucc cuggaauuuc gaccaaaaag uagauaggcu cgcuucggcc     1560

aaccaguuua uccgccagaa uaugccaaag gacaaccauc ugcuugaggc agaugugcug     1620

ccugccaauu cuuugacaua ccagcgguau cucguccugg acgaauuaaa cgcugugaag     1680

guuaaucaca aaucccugac cgugucacaa aagcagagaa uuuucaacga ucuauuuaaa     1740

aggcagcgaa cggucaccgu gcucaaucug caaaacuacc uuaagaguau agagaaauug     1800

cccacacugc caguaaucac ugggguuagc gacuccgagc gcuucaagac cacacucucu     1860

acuuaucugg auuuuaaaaa uauuuucgga gguaaggugg acgauagcuc acagaaggaa     1920

aaauuugaga agaucaucuu auacgccacc auuuucgaag accacgcagu cuauaaagcg     1980

aagcuggauc agauagacug gcuuggccag caacagaaaa cagacuugcu caguaagaac     2040

uaccaggggu ggggacagcu guccgagaag cuacugcucg gccugaaaaa ccaagcuggu     2100

cagagcaucc uugaugaguu guggcagacc aauaagcggu uuagagaaau uaugaacgag     2160

gcccauuucg caaaacagau cauggccucu aaugcugccc uguuaaggaa gaaagggcuc     2220

gacgaugugc ugucaacugc auuucuuagu ccccaaaaca agaaggcuau ucgucagacg     2280

gucgaagugg uugacgauau cgugaaagcc auaggcuguc cuccacagaa gaucagccug     2340

cacuucucca aagcgccggc cgacgcuauu cgccaugagc gggauaaugc aaacaucauu     2400

uugagaacau auaggaaucu ccccgccaag augguagacg aucaacugaa agcugaacua     2460

caggccaccg cagagaagca ccagguccug ucugacaagc ucuaccugua cuuuacucag     2520

cuuggacgag auuuguauac cggcgagaug cugaaaaugu cggaauuaga gaacgacaug     2580

aucgaucaca uaaugccuca aacauucgcg uacgacgaca gcgugaauaa ccggguucuc     2640

gugucccaga aucauucaag ucagcugggg aagaccucug gagaaaaauc ugaugccaag     2700

augauucgcu ucuggcaggu ccuuagagac aacaaacuga ucuccgagca aaaguaucag     2760

aaucuaacuc ucgauucaga ccaccugagc aaaucuacaa gggaacaguu gcugaaccgg     2820

uacuuagugg agaagaaucc aauuacgcag uggguagcuu ccgugcucac cgagaaguau     2880

ggugauaacg gcacucgagu ucugacaauc aaccccagaa uggucuacca aaugcgcagg     2940

caguuuaauc uugugaaccu gccugaagug aaugacuaug aucauggguu ggacgccuac     3000

cucgcagcuu ucauaggaaa cuacgucgau cgucgguauc caagacugag gaguuacuuu     3060

guuuauggcg acuucagcag cuccaaagag aucguguuuc gccuaaaucg auucaacuuu     3120

cuguucgauc uuggucaggc cgaacccaau gagcucaccg acugggaaac aggggagaug     3180

auuugggaca aggcagccgc uaacucagug cugagagaug uauaccagua uaaauuuaug     3240

uugaucaguc aagcggucua cagccggcag ggcgccaugu ucaaucagac uauuuauccg     3300

gcaucgcaag cugccggaag gcagcugauc ccugugaaga aaaaccgcgc caccaaguua     3360

uacggcgggu acuccggaaa uguugacgcu uaucucgcaa uagugcgucu ggguggccca     3420

aagucugauc aguacagagu cguggggauu cccacgcgga ugguuggaag gcuucagagc     3480

gccaugaaac aaggcgcgga caccuaugcu aacacacugc acaagaucuu gcagccucag     3540

uuuacuaaac gcaagaaaaa ucgacagacc gguuacuaug aggaaacaac ugagcccuuc     3600

gccgugaucc ucgauaaggu auacuauaga caacugauua ucgacgggca gcagaaguuc     3660

augcuaggcu caaguaccua ccaguuuaac gcaaaacaac ugguccuuuc cucucgggcc     3720

aggcagauuc ucagcggaga ggagcgccug acggaugaga auggcuccca ggacuugaua     3780

gauguguacg acgaaaucuu aacaaagauu gauagauauu ucgagcugua cgaccaaagg     3840

cacuuucggc agucacucca ugaugggcgu aaacaguuca ucagccugcc aauucacaac     3900

guguaucgcg cuaaccgacu uaucgaacau ggaaaguacg agacccugag ucagauauug     3960

aauggccuac acgcaaacac uaugaugaca aaucugagau aucucgguau cucuuccccu     4020

uuugggaaau uccaaagccc caacggaauu aagcugucga aggacgccgu ccuuguuuac     4080

cagucaccau cuggcuuauu ugaaaggcgg gugcgccuga gagaccucug a              4131


<210>  3183
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3183
augaacaaua agcccuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucauaacag augacuauaa aguuccuucu aagaaaauga aggugcucgg uaauacugau      120

aaacacuuca ucaagaagaa ccuucugggc gcuuuguuau uugacgaggg gacgaccgca      180

gaagauagaa ggcugaaacg gacagcccgc cgacguuaca cuagaaggaa gaaucggcua      240

cgcuaucucc aggagauuuu caccgaagag augucaaaag uagacaucag cuuuuuccau      300

agacuggaug acaguuuucu ugugccagaa gauaagaggg gauccaaaua cccgauuuuc      360

gcgacacugg aggaggaaaa ggaguaucac aagaacuuuc ccaccaucua ccauuugcgg      420

aaacaccucg cugacucuaa ggaaaaagcc gauuuccgcc ugauauaucu ggcacucgcc      480

cauaucauua aguaccgagg ccacuuuuua uacgaggagu cguucgacau caaaaauaac      540

gauauucaaa agaucuuuaa ugaguucaua ucaauuuaug acaacacuuu ugaagguagc      600

agucugaaug ggcagaacgc ucaggucgaa gcaaucuuca cagacaaaau uuccaagucu      660

gccaagagag agagggugcu uaaauuguuc ccugaugaaa agagcaccgg acuguuuucc      720

gaguuccuaa aacucaucgu uggcaaucag gcugacuuua agaaacacuu cgaucuggag      780

gaaaaggccc cacuucaauu uucaaaggac acguacgaug aggaccugga aaacuugcuc      840

gugcagaucg gcgaugacuu cgcggaucug uuuuuagucg cuaaaaagcu guaugacgcc      900

auucuccuua gugggauacu gacugugacc gaucccagca caaaagcucc uuugucugcc      960

uccaugaucg accgguacga gaaucaucag aaggaccuag cagcucugaa acaguucauu     1020

aagacuaacc ucccagaaaa guaugaugag guauuuucag accaaagcaa agauggauac     1080

gccgguuaua ucgacggcaa gaccacacag gaggcguucu acaaauacau aaagaaccug     1140

cuuaguaaac uggaaggggc cgauuacuuu uuggacaaga uugagcguga agauuuccuc     1200

cgcaagcaga gaaccuuuga caauggaucu aucccccacc agauccaucu gcaagagaug     1260

aacgcaauua ucaggcggca gggcgaacac uauccuuucu uacaggagaa uaaagagaag     1320

auugaaaaga uacugacguu ccgaauccca uauuacguug guccgcuagc ucgcgggaac     1380

agagauuuug ccuggcucac uaggaacucc gaccaagcua uucggcccug gaauuucgag     1440

gaaaucgugg auaaggcccg cagcgcagag gacuuuauca auaaaaugac aaacuaugau     1500

cuguaccuuc cugaggaaaa ggucuugcca aaacacucgc ugcucuauga gaccuucgcc     1560

guguacaaug aacugacuaa ggugaaguuu auugcugagg gacuuagaga cuaccaguuc     1620

cuggacucug gccagaaaca gcaaauaguc acacaguugu uuaaggagaa aaggaagguu     1680

accgaaaaag auaucauuca guaucuccau aacguggacu ccuacgaugg caucgagcug     1740

aaggggauug aaaagcaguu caaugcguca uuaaguacgu aucacgaccu gcuaaaaauc     1800

auaaaggaua aagaguuuau ggacgauagc aagaacgaag caauccucga gaauauugua     1860

cauacccuga caaucuucga agaccgugag augauuaaac aacggcuugc ccacuacgcu     1920

uccaucuuug augagaaggu gauaaaggcc uugacucgaa gacauuauac cggauggggu     1980

aaacugucug caaagcucau uaacggcauc uacgacaaac agagcaagaa aacaauucug     2040

gauuauuuaa ucgacgaugg ggaaauaaau aggaacuuca ugcagcugau caacgacgac     2100

ggacuuucau uuaaggagau uauccagaag gcccaagucg ugggcaaaac uaaugauguu     2160

aaacaggugg uccaggaauu gcccgguagu ccugcuauua aaaaggggau ccuccagucc     2220

auaaaacugg uggacgagcu aguaaagguu augggccacg ccccagaaag cauugugauc     2280

gagauggcac gcgagaacca aaccacagcg cggggaaaga aaaauucuca gcagagauac     2340

aagaggaucg aagaugcucu gaaaaaccuc gcccacggcc uggacucaaa uauucuuaag     2400

gagcauccca ccgauaacau ccaauugcag aaugaccgcc uguucuuaua cuaucuccag     2460

aacgggaaag auauguacac uggaaagagu cuggacauua aucagcuuag cuccugcgau     2520

auagaccaca ucauuccgca agcauucauc aaagaugacu cucuggacaa ccgagucuug     2580

acgucgagca aggauaaucg ggguaagucc gacaacgugc cuucacucga aauuguccag     2640

aaacguaagg ccuuuuggca gcagcugcua gauaguaaac ugaucucuga gagaaaguuc     2700

aauaaccuca caaaagcuga gaggggcggg cuggacgaac gcgauaaggu gggauuuaua     2760

aagcggcaac uuguugagac cagacagauc acuaaacaug uggcccagau uuuggacgca     2820

agguucaaua ccgaaguaac agagaaggau aaaaaggacc gaagcgucaa aaucauuacc     2880

cugaagucca acuuaguguc aaacuuucgc aaggaguucc ggcucuauaa aguuagagaa     2940

aucaaugauu accaccacgc ucaugacccc uaucugaacg ccguggucgc gaaggccaua     3000

cuuaaaaagu acccaaaacu ggagccugaa uuuguguaug gcgauuacca gaaguacgac     3060

cuaaagaggu auauuagccg uacuaaagau cccaaggagg uagaaaaagc uacagagaag     3120

uacuucuuuu auucuaaucu ccugaacuuc uuuaaagaag aggugcacua cgcagacggu     3180

acgaucguua agcgcgagaa uaucgaauau uccaaagaca ccggggagau ugccuggaac     3240

aaggaaaagg auuucgcuac uaucaaaaag gucuugaguc ugccacaagu gaauauagug     3300

aaaaagacag aggaacagac cgucggccag aacggaggcu uauucgacaa uaacauuguu     3360

agcaaaaaga agguggugga ugccucuaaa cucacaccca ucaaguccgg gcugucaccu     3420

gagaaauacg gagguuaugc acggccaacu auugcguaca guguacuugu caucgccgac     3480

auagagaagg gcaaagcuaa gaagcugaaa agaaucaagg aaaugguggg gauuaccguu     3540

caagauaaaa agaaauuuga ggccaauccg aucgcauacu uggaagagug uggauauaag     3600

aacauuaauc ccaaccucau cauaaagcug ccuaaauaca gccuauucga guuuaauaac     3660

ggccagaggc gacugcuugc uucguccauu gaacuccaga aggguaacga gcugaucgug     3720

ccauaucauu ucacggccuu gcuguaccac gcacagcgga ucaauaaaau uucugaaccc     3780

auccauaagc aauaugucga gacccaccag agcgaauuua aagaguuacu cacagccaua     3840

auuucacuga guaagaaaua cauccagaag ccuaacgugg agucccuucu gcagcaagca     3900

uucgaccagu cugauaagga cauuuaucag uugagcgaau ccuuuaucuc acuccugaaa     3960

cuaauuagcu ucggggcgcc aggcacuuuu aaguuccugg gaguugagau aagucagucu     4020

aaugugcgcu accaauccgu aagcucgugc uuuaacgcca cccuuaucca ccagucaauc     4080

acaggccucu acgaaacuag aauugaucug ucuaaauugg gggaggacug a              4131


<210>  3184
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3184
auggacaaga aauacggcau cgggcuggau auuggaacca gcuccauagg uuggacagug       60

gucgacaagu auggccacau cauuagaguu aaagggcaga cuggaaucgg cgugaggcuc      120

uuucgggagg gucaaacggc cgcugaacgc cgaaccuuuc guacaacuag aaggcggcuu      180

ucucgcagaa gguggcggcu gcgcuuguua cgagagauuu ucgaugaacc caucucagca      240

auagacgaga acuuuuucgc cagacagaag gcgaguagcc uggcuccugg ggaugccagg      300

uaccagcagg cauuucaacu auucaaugac cggaccgauc aggccuuuua uugcaaauac      360

ccaacaaucu aucaucuccg ucaggcucug augaccgaau cccgccaguu cgacauuaga      420

gagaucuacc uugcacugca ccaaauuauc aaguauaggg gacauuuucu ucucggcggc      480

gccgcuaagg auuuccaguc ugacaaacug gaucuggagg ccuacuuuac uacaauaaac      540

gcgcagcucg cagggauuua cucggacgga ucaguacagu uaccgacccu ggauuggaau      600

caagccuguc aggaacuuac ggacacuagc cggacccaga gugaccgaaa gaacugguug      660

gugaaagagc ugaaguccua uggugauucu caacuaaaac ccaucuacac acagauugcu      720

aaugccaucc ucggcuucaa gacugaccug gcaaagauca uggggauuaa cgauccugaa      780

aaagcucuua gcuuuaaucu ggacgccuug gaugaccacc gcgaugacgu cgcggagcuc      840

cugaccgaug agcagauacc auuagugaac cugcucgcca aguugcauac agcacuggaa      900

uuggcugcca uccuacccga cggacagacc cuguccggca aaaugguuga gauuuaugaa      960

acgcacagaa agcaacucaa aacuuacaag gcuuucauca ggcagcugcc ugaucggcag     1020

cagcgccuug cccugaaaca agcauaugac cacuacauag accaggguuu gaauagagag     1080

gccuucuauu cagaagugaa ggcuauucuc gagaagcaga aaccacugac agcgaccggg     1140

caggcaauga agaaagccau ggaucaaaac gcuuucuuac ugaagcagag gacuaaaaca     1200

aauaucgcca uccccuacca gguccagcaa caggagaugg accagauuau cgcaaaccaa     1260

aguaaguacu auccuuggcu agccacccca aauccgguaa agagccgucg ggugauugcu     1320

cccuacaaac ucgaugaacu gguuugcuuc cgagugaacu auuacgucgg accucuugug     1380

cagucuaagc caggcgaggc cacgaaagaa gcaaaguuug cguggaugac cagaaaagac     1440

ccccagucca caguggcuau aacuccuugg aauuucgauc agcaagucga caggcaggcc     1500

ucagcaaacg ccuuuaucca gcgcaugaag accacagaua cuuauuugcu gggcgaggac     1560

guuauuccaa agaacagccu ccuguaccag aaauaugaag ugcuuaauga gcugaacaau     1620

guacggguga acggggauug guuggacccc caaagaaagc aguaccucuu caaaaagcug     1680

uuuauggauc agccgcagag ugucgugacc guuaaggcuu uacaaaagac acuggugguc     1740

aucggagccu guccuacccu acccacugug gagggucucg caaauccaaa gagguuccug     1800

ucucagcuua cgacauacca ugacuuuugc aaauugcugc cugaugacau ccagcgcgcu     1860

gaccgacaag ccgauauuga aaagauaauc aacuggucca ccauuuucga ggacacugcg     1920

aucuuuaaag aaaagcuccg ggauauuaaa uggcugagcg ccgaccagau ccguagauua     1980

ucgucuaggc cccgcuaucg gggcuggggg agauucuccg cuaagcugcu uuguggauug     2040

cacgauaggc agggcaauac cguaauggac gagcucuggc agacaucacg aaacuuuaug     2100

caaauaaucc gccagaguga uuuccagacc gcaauugccg cuguuaauca gcaagaccug     2160

ggucggcagg aucuacagag agugaucgac gaucuguaca cuagcccaca gaacaaaaag     2220

gccauuaggc aaguccucgc aguggucaca gacauccaga aggcgaugca ugggaaagcc     2280

cccuccuggc uguucgugga aguugcuaag ggcgcccaga aaaauccuca gcguacgcaa     2340

ucucgccaga gcaaacuugc agaggcuuau cgggagucag ccagagaauu gguggaugag     2400

gcaguaaaag aacagcuguu aacccuccca gacgacaggc aaacuuuuag ugaucgacug     2460

guccuuuacu uccagcagaa cggacgggac auauauacag gcgagccgcu ggauuuugag     2520

cgcuugucca auuaccacau ugaccacauc auuccccagg ccgugguuaa ggaugacagc     2580

cucgauaaca gagugcugac cucuaaucaa gcuaaccuag aaaagggggc gcaugucccu     2640

acaaucuuca gggacaaaca ggagccagug uggcaggaac uguaucgggu aggacucaua     2700

ucacaccgaa agcugagaaa ccuuuugacu acccccgaug accuggauga ccgcgguagg     2760

gccacgggcu uuaucgcacg ucaguuagug gagacccggc aaguuauuaa acucgucaug     2820

gaagugcuga gucaguacua cgacacaacu gagaccgagc uuaucagcau uaaggcuggg     2880

cugucccauc aguucagaca ggaacuaaau cucccuaaac ugagggccuu gaacgauuau     2940

caccaugccc aagacgcuua ccuggcagcc cgcaucggaa cauauuuacu ccagcgauac     3000

ccaaaucuga agagauuuuu cguguauggc gauuuuaaga aaacucacuu cgaccuucag     3060

cggagguuua acuucauagg ugggaugauu ucucgcgaga agcaacccua caaucugauc     3120

aaaaacccgg ucacccacca gguuaucugg gaugaagaga uggaaauugg cgacugccag     3180

aagaaaaauc cugagcagcc aaagcaugau gcgacgguga agaugaugaa cuugacaaaa     3240

aucaugaauc ucaagcacau ucuggugacc caugagguau augaaaacca cggagcucua     3300

uuuaaucaaa cuauauaccc cgccucgcag gcacacaaaa agaaacugau cccucagaag     3360

acagagaaac ccauugaacu uuacggcggg cauagcuccg agcaggccgg auauauggcu     3420

aucauucguc uccuggguaa gagaccaggc uacaagguca ugcgggugcc uguuagguau     3480

gugucacaau ugcagcacca ggacgucgca gaacagaaaa agcuguuaua caaaaucuuc     3540

gugagugagc ucaccucucg caacaagguu aaacccuucg aggugguagu cccacaugug     3600

ugucugaauc aaccggugga agauaugaua aaggggcagu ggcaccgauu ugcccuuaga     3660

acuaacggau auuaccagaa ucagcaacag cugaucuuga gccucgucgc gcagcggacc     3720

cuggcuggcu auaagccuaa agacgaugag cuaauggccg uuuaccauga aauuugcaag     3780

caagugaaau acuauuuccc ccuguacaag augaacaaau uucaguccag gcuuuucgca     3840

gccgaggagc uccugcagaa gcgcucaaca guaaacagcu uuucuaucac gauugguaag     3900

aaugggaaag aaaacaagac cuugaauuua ggcaaaagag agcugcuccu gaggcuucug     3960

ggaggcuugc acgcuaacgc cacucgggug gaccuaaagg auaucgggug uacaggacag     4020

uucggccaac ugcaggucaa aaaugguaua aagcucaccg aacaggcagu gcugauucac     4080

gaguccccaa cagggcuuuu ucaucgucgc guucgauuaa acgaccugug a              4131


<210>  3185
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3185
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucauaacug augacuauaa gguucccgcu aaaaagauga aagugcucgg uaacacggau      120

aagaaguaca ucaaaaagaa ucuucugggc gcauuguuau ucgacucugg ggagaccgcc      180

gaagcgacaa gacugaaaag gacugcucgg cgccgauaua cccguagaaa gaacaggcua      240

cgguaccucc aggagauuuu ugccgaagag augacaaaag uagaugaauc auucuuuuau      300

cgccuggacg agaguuuccu uaccacugau gagaaggacu uugaaagaca cccuaucuuc      360

ggaaauaagg cagaugagau uaaauaccau caagaauuuc caacaaucua ucaccugagg      420

aagcauuugg ccgacagcuc cgagaaagcu gaucuccggc ugguguaccu ggcacucgcc      480

cacaugauaa aguuccgcgg ccacuuuuua aucgagggug aacugaacgc ugagaauacc      540

gacguccaga aaauuuucgc cgauuuugug gggguuuacg accgaacguu cgacgauucu      600

caccuuucgg aaaucacugu ggacgcggca ucaauuuuga ccgagaagau cagcaaaagu      660

agaaggcugg aaaaccuaau aaaguauuac ccgacagaga agaaaaauac ucucuuugga      720

aaccugauug cccuugcucu gggcuugcag cccaauuuca agaugaacuu caaacucucc      780

gaggaugcca agcugcaguu uucuaaagac agcuauaaug aagauuuaga ggaacugcuc      840

ggcaagaucg gggacgauua cgcagaccuu uucaccuccg cuaagaaccu guaugaugcc      900

auuuugcuau caggaauccu gacagucgac gauaauagua ccaaagcgcc ucucagcgcc      960

ucuaugauca agcgguacgc agagcaccau gaagaccugg agaaacuuaa ggaguuuauu     1020

aaagcuaaca aguccgaacu guaucacgac auauucaagg augagacgaa aaacgguuac     1080

gccggcuaca ucgaaaaugg ggugaagcaa gacgaguuuu auaaauacuu gaagaacacu     1140

cucucaaaaa uugcuggaag cgauuauuuc cuggacaaga ucgaacguga ggacuuuuua     1200

cgcaagcaga gaacauucga uaauggcagu auaccacauc agauucaucu gcaggagaug     1260

cacgccaucc uaaggcggca aggugauuac uaucccuuuc ucaaagaaaa ccaggaccga     1320

aucgagaaga uucugaccuu ccgcaucccu uacuauguag ggccacuugc aagaaaagau     1380

ucuagguuuu ccugggccga auaccauagc gacgagaaga uuacucccug gaauuucgau     1440

aaaguuauag acaaggagaa aucggcugaa aaguuuauca cacggaugac cuugaacgau     1500

cuguaccucc cugaggaaaa ggugcugcca aaacacucuc augucuauga gacguacgcg     1560

guguauaaug aacuuaccaa gauuaaauac gugaacgagc agggaaagga cuccuucuuc     1620

gacucaaaua ugaaacagga gaucuuugau cacgucuuca aggaaaaccg caagguuaca     1680

aaagagaagc uguugaauua ucucaacaaa gaauuuccgg aguacagaau caaggaccug     1740

auuggcuuag auaaagaaaa uaagaguuuc aacgcaagcc ugggcacuua ucaugaccua     1800

aagaaaauac ucgauaaggc cuuucuggac gauaaaguga augaggaggu aaucgaagac     1860

auuaucaaga cccuuacauu guucgaggau aaagacauga uucacgaaag gcugcaaaag     1920

uacuccgaua ucuuuacugc ugaccagcuc aagaaacugg agcgucggca cuacaccggg     1980

uggggacgau uaucuuauaa gcugauaaac gguaucagaa acaaagaaaa uaacaagaca     2040

auucuugauu acuugaucga cgacggcagc gccaauagga acuucaugca gcucauuaau     2100

gaugacaccc ugcccuuuaa acaaaucaua cagaagucac agguggucgg ggauguggac     2160

gauauugagg caguugugca ugaccuaccu ggaaguccag ccaucaaaaa gggcauucug     2220

caguccguca agaucgugga ugagcucgua aaaguuaugg gugacaaccc cgauaauaua     2280

gugaucgaaa uggcucgcga gaaccaaacu acgaaucggg ggagaagcca gucucagcag     2340

aggcugaaga aacuucaaaa cucauugaag gaacugggca guaauauuuu aaacgaggag     2400

aaaccuagcu auaucgaaga caaggucgaa aauucccacc uccagaacga ucagcuguuc     2460

cuuuacuaua uucagaaugg aaaggacaug uacacaggcg acgaacugga uaucgaccau     2520

uugucugauu augacauaga ucacauuauc ccacaagccu uuaucaaaga cgauucgauu     2580

gacaaccgcg ugcucaccag cuccgcaaag aaccgaggga aaucagauga cguccccagu     2640

cuggauaucg ugcgggcgcg uaaggcugag uggguuagac uauacaaauc uggacugauu     2700

agcaagagga aguucgacaa ucucacuaaa gccgaacgcg guggccugac cgaggcagau     2760

aaggccgggu uuauaaaacg gcagcuugug gagacaagac agaucaccaa gcacguagcu     2820

cagauuuugg acgccagguu caacacugaa uccgacgaga augauaaagu cauccgagac     2880

gugaagguua uuacacugaa gucaaacuua gugagccaau uccgcaaaga uuuugaguuc     2940

uacaaggucc gggagaucaa ugacuaucau cacgcacaug augcuuaccu caacgccgug     3000

guaggaacgg cgcugcuuaa aaaguauccg aaacuggccu cugaauuugu guacggcgag     3060

uauaagaagu acgacguuca caaacuaaua gcuaagucca gugaugacca uagcgagaug     3120

gguaaagcaa ccgccaagua uuucuuuuac ucuaaucuca ugaacuucuu uaaaagaguc     3180

aucagguacu ccaaugggaa ggugauugug cguccugucg uugaauauuc aaaagauacu     3240

gaggacaucg cuugggauaa gaagaguaac uuccgcacaa uuugcaaagu gcugagcuac     3300

ccccagguga auaucguaaa gaaagucgaa acccagacag gcggauuuuc gaaggagucc     3360

auauugccaa aaggcgacuc ugauaagcug auuccucgga agacuaaaaa ggccuauugg     3420

gacaccaaaa aguacggggg auucgacagc cccacggugg cauauucagu uuuugugguc     3480

gcggaugugg aaaaagguaa ggccaagaaa uuaaagaccg uuaaagagcu cgugggcauc     3540

aguaucaugg agagauccuu cuucgaagag aacccaguag aauuucugga gaauaagggg     3600

uaccacaaca uuagggagga caaacuuaua aagcugccca aguauucuuu guucgaauuu     3660

gagggaggca aacgacggcu ccuggcuagc gccuccgaac uacaaaaggg uaaugagaug     3720

gucaucccug ggcaccuggu gaaacuucuc uaccaugcac agcgcauuaa cucauucaac     3780

agcacaaagu accuggauua ugugagugcu cacaaaaagg aauuugagaa agucuugucu     3840

uguguugagg acuucgccaa ucuguacgug gauguagaaa agaacuuauc caagaucaga     3900

gcaguggccg acagcaugga uaauuuuucg auugaggaaa ucucaaacuc uuucauaaau     3960

cuccugacuc uuaccgcucu gggcgcgcca gccgacuuua acuucuuggg agagaaaauc     4020

ccgaggaagc gguauacaag uacuaaagaa ugccucaaug caacccugau ucaucagucc     4080

aucacgggcc uauacgagac acgaauugau cugagcaaga ucggggagga auga           4134


<210>  3186
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3186
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

auaacagaug acuauaaggu uccuucuaaa aagaugaaag ugcucgguaa uacugauaag      120

cacuucauca agaaaaaccu uauuggcgcu cuguuguuug acgaggggac gaccgcagaa      180

gauagaaggu uaaagcggac aucacgccga cguuacacua gaaggaaaaa ucggcugcgc      240

uaucuacagg agaucuucag ugaagagauu agcaagcucg acuccucuuu cuuccauaga      300

cuggaugacu cguuucuugu accagaagau aaaaggggau caaaguaccc gaucuucgcc      360

acccuggagg aggaaaagga guaucacaaa aaguuuccca caauauacca uuugcggaaa      420

caccucgcgg acagcaagga aaagaccgau cugcgccuga ucuaucucgc uuuagcccau      480

augauuaagu accgaggcca cuuccuguac gaggagaguu uugacaucaa aaacaaugau      540

auucaaaaga ucuucaacga auuuauaucc auuuaugaca auacuuucga ggguucuagc      600

cuuuccgggc agaacgcaca gguggaagcc aucuuuacag acaagauuuc aaaaaguacc      660

aagagagaga gggucuugaa acuguucagc gaugaaaagu cuacgggacu auucuccgag      720

uuucucaaac ugaucguggg caaucaggcu gacuucaaga agcacuuuga ucuugaggaa      780

aaagcaccuc ugcaauucuc aaaggacacu uacgaugagg acuuggaaaa ccuccugggc      840

cagaucgggg auggauuugc cgacuuauuc cugguugcua aaaagcucua ugaugccauu      900

cuucugagcg guauauugac cgugacagac ccaaguacua aagcgccccu aucugcaucc      960

augaucgagc gguacgaaaa ucaucaggag gaucuggccg cucucaagca guuuauuaag     1020

aacaaucuga gcgagaaaua ugccgaaguc uucucggacc aaucuaagga cggcuacgca     1080

ggguuuaucg auggaaaaac cacacaggag gcuuucuaua aguacauuaa aaaccuucug     1140

uccaaguugg aaggcgccga cuacuuucuc aauaagauag agcgugaaga uuuccugcgc     1200

aaacagagaa ccuuugacaa cgguucaauc ccucaccaga uccauuuaca agagaugaac     1260

gcgauucuga ggcggcaggg ggagcacuau cuauuccuca aggaaaaucg agagaaaauc     1320

gaaaagauuc uggccuuccg cauaccauac uaugugggac cccuugcaag aggcaacagg     1380

gauuuugcuu gguugacgcg gaauagugac caggccaucc gcccuuggaa cuucgaggag     1440

guaguugaua aagcuagcuc cgccgaagac uuuauuaaua agaugacuaa cuacgaucug     1500

uaucucccag aggaaaaagu gcugccgaag cacucucuuc uguacgagac auucgcaguc     1560

uauaaugaau ugaccaaggu gaaauuuauc gccgagggcc ucagagacua ccaauuccug     1620

gauagcgggc agaagaaaca gaucgugaac caguuauuua aggacaaaag gaaggucacu     1680

gagaaggaua uuauacauua ccugcacaau guugacggau augacgguau cgaacuaaaa     1740

ggcauugaga agcaauucaa cgcuucacuc aguacauacc augaucugcu uaaaaucauu     1800

aaggacaaag aauuuaugga uaaucccaag aacggggaga ucuuggaaaa uauaauccac     1860

acccugacga uuuucgagga ccgugagaug aucaagcagc ggcucgcgca guaugauacc     1920

cuguuugacg aaaaagugau uaaggcauua acacgaagac auuacacugg auggggcaaa     1980

cuguccgcca aacuuaucaa cgguauaagg gauaaacaga ccgggaagac aauuuuggag     2040

uaucucaucg acgauggcga cugcaaucgc aacuucaugc aacugauuaa cgaugacgga     2100

cugagcuuua aggaaaucau acagaaagcu cagguagugg gcaagacuga ugacgucaaa     2160

caggugguuc aagagauccc ugggucucca gccauuaaga aaggaauccu gcagucaauu     2220

aagaucgugg acgaacucgu caaagugaug ggucacaauc ccgagaguau aguaauugag     2280

auggcacggg aaaaccagac cacagccaag ggcaagaaaa auagccagca aagauacaag     2340

aggaucgagg augcucugaa aaaccuugcc ccuggguugg acuccaauau ccugaaggaa     2400

aacccaaccg auaauauuca guuacagaac gaccgccucu uccuguauua ccuucaaaau     2460

ggaaaagaua uguacacugg caaggcaauc gacauuaacc agcugucuaa uuaugauaua     2520

gaccacauca uuccccaggc guucaucaag gaugacucgu uggauaaccg aguucucacg     2580

agcuccaaag acaaucgggg uaagucagac aacgugccga guauugaggu cgugcagaaa     2640

cguaaggcuu uuuggcaaca gcugcuagau ucuaaacuga ucagcgagag aaaguucaau     2700

aaccucacaa aggccaaacg cgggggccug gacgaaaggg auaaggucgg auuuauaaaa     2760

cggcagcuug uggagaccag acagaucacu aagcauguug cacaauugcu ggacaccagg     2820

uucaacacag aagugaauga ggaaaaccag aaaauucgaa ccguaaagau cauuacuuua     2880

aaguccaauc ucgucucaaa cuuucgcaaa gaguucggcc uguacaaggu gcgggagauc     2940

aaugauuauc accaugccca cgacgcuuac cuuaacgccg uuguggcaaa agcuauacug     3000

aagaaauauc cuaagcuaga acccgaguuu gucuacgggg auuaucagaa guacgaccuc     3060

aaaagauaca uucuaaagag caaagccucu aauacaaucg auaaggcgac ggaaaaauau     3120

uucuuuuacu ccaacuugcu gaauuucuuu aaggagaaag ugagguaugc cgacggaacc     3180

aucaagaagc gugaaaacau ugaguacagu aaugauacug gugagauagc uuggaacaaa     3240

gaaaaggacu ucgcaacaau caaaaaggua uuaagccucc cacaggugaa uauuguuaaa     3300

aagaccgagg aacaaacagu cggccagaac gggggacugu ucgauaauaa caucgugucu     3360

aagaaaaagg uggucgacgc cuccaaacuu auuccuauca agucaagucu gagccccgag     3420

aaauauggcg guuacgcucg cccaacuaua gccuauucgg uuuuggugau cgcagacauu     3480

gaaaagggga agggcaaagc gaagaaacuc aagcggauca aagagauugu gggaaucacc     3540

auacaggaua agaagaaauu ugaguccaac cccguaacgu accuggaaga guguggcuac     3600

aagaauauua acucuaaucu aaucaucaaa cugccuaagu auagccuuuu cgaauuuaac     3660

gacgggcaaa gaaggcuccu ggccucaagu auugaguugc agaaaggaaa ugagcugauc     3720

uuaccauacc accucaccgc ucugcuuuau caugcccagc gaauaaacaa gauuuccgaa     3780

ccgauccaca aacaguacgu cgaggcacau caaaaugaau uuaaggagcu guugacaacu     3840

auuaucucuc ucagcaagaa auauauacag aagcccaacg uggaacugcu acugcagcag     3900

gcuuuugauc aagccgacaa agauauuuac cagcuuuccg agucauucau cagccuccug     3960

aaguugacca guuuuggcgc accuggugcc uucaaauuuu uagggguuga gaucucucag     4020

uccagcgugc gguauaagcc aaauucgcag uuccuggaca caacucucau ucaccaauca     4080

aucaccggac uguacgaaac gcgcauugau cuuucuaagc ugggcgagga cuga           4134


<210>  3187
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3187
augaacaaua agcccuacag caucggccug gacauuggga ccaacuccgu gggauggucu       60

gucauaacag augacuauaa aguuccuuca aagaaaauga aggugcucgg uaauacugau      120

aaaagauuca ucaagaagaa ccuuauuggc gcccuguugu uugacgaggg gacgaccgcu      180

gaagcaaggc gguuaaaacg cacagcccga cguagauaca cuaggcggaa gaaucgccug      240

agauaucuac aggagaucuu cgcggaagag augaguaaag uagauagcuc cuuuuuccac      300

aggcucgacg auucuuuucu gauuccagaa gacaagcggg gaucgaaaua cccgaucuuc      360

gcuacccuug uggaggagaa ggaauaucau aagcaauuuc ccacaauaua ccaucugcgc      420

aaacaguugg ccgauucaaa ggagaaagca gaccuccgac ugaucuaucu gguccucgcc      480

cacaugauua aguacagagg ccacuucuua uacgaagagg cuuuugauau caaaaacaau      540

gacauucaga agaucuucaa cgaguuuaua agcauuuaug auaauaccuu cgaagguagu      600

ucccugucug ggcagaacgc acaaguggag acuaucuuua cagacaaaau uagcaagucc      660

gccaagaggg aacggguucu uaaauuguuc ccugacgaga agucaaccgg acuguucagu      720

gaauuucuaa aacucaucgu gggcaaucag gcugauuuca agaaacauuu ugaccuggag      780

gagaaggccc cacuucaguu cagcaaggau acguacgacg aagaucugga gaacuugcuc      840

gaacagaucg gcgacgauuu ugcggaccug uucgucgcag ccaaaaaguu auaugaugcu      900

auucugcucu cugggauacu uacugugacc gaccccucca caaaagcccc ucugucagca      960

agcaugaucg agcguuacga aaaucaccaa aaggauuugg cuguacuaaa acaguuuauu     1020

aagacuaacc ugccagagaa guauguugag guguucagug accagucuaa agacggauac     1080

gccgguuaua ucgauggcaa gaccacacag gaagcguuuu acaaauacau aaagaaucuc     1140

cuguccaaau ucgacggggc cgauuauuuu cuugacaaga uugagcgcga agucuuccug     1200

agaaagcaaa ggaccuuuga uaacggaagc aucccccacc agauccauuu gcaggagaug     1260

aacacgauuc uccggcgaca aggcgaacac uacccuuucc ugaaagagaa uaaggagaaa     1320

aucgaaaaga uuuuaacuuu ccgcauacca uaucaugugg guccgcuggc aagagggaac     1380

agggacuuug cuuggcuaac acgcaauucg gaucaggcca uccggcccug gaacuucgag     1440

gaaauugugg acaaggcuuc uuccgccgag gauuuuauca auaagaugac caacuacgac     1500

cucuaucugc cugaggaaaa aguccuucca aagcacucau ugcuguacga gacuuucgca     1560

guuuauaaug aacucacaaa ggugaaauuu aucgccgagg gaaugagaga uuaccaguuc     1620

cuggacagug gccagaagaa aaagauuaua aaccaacuuu uuaaaaagaa gaggaaagua     1680

accgaagaug acaucauuga cugccugcag aagaucgaua auuacgacgg cauugaguug     1740

aaagggaucg agaagcaguu caacgcuagc cucuccacgu aucacgauau acugaaaauc     1800

auucgugaca aggaauuuau ggaugacccc aagaauggag auaucuuaga gaacgugauu     1860

cauacccuga caaucuucga agaccgggag augauaaaac agcgacuagc gcaauacaau     1920

ucuauuuuug augaaaaggu caucaaagca cucagcagaa agcacuauac ugguuggggc     1980

aaacugucag ccaaacuuau ugacgggauc agggauaagc agaccggaaa aacaauauug     2040

gacuaccuga uggaugacgg caagaguaac cgcaauuuca ugcagcucau caaagacgau     2100

gagcuguccu uuacugagau uauccagaag gcucaagugg uuggugacac cgauaacuua     2160

agacaggugg uccagaaccu gccugggagc ccagccauua aaaagggcau ccuucagucu     2220

auaaaaauug uggacgaauu gguaaagguu augggacaug cacccgaguc aaucgugauc     2280

gaaauggccc gggagaauca aacaaccgcu aggggcaaga aaaacaguca gcagcgcuau     2340

aagcgaauug aagauagccu caaaaaucug gccuccgggc uagacucuaa cauccugaag     2400

gagaauccga cugauaacau ucaacuccag aaugaccggc uguuccuuua cuauuugcag     2460

aacggaaaag auauguacac gggugaggca cugaauauaa accaguuauc gagcuacgac     2520

aucgaucaca uuaucccuca agcguucauu aaggacgacu cccucgauaa ucguguccug     2580

acaucaagua aggacaacag aggcaaaucu gauaaugugc ccagccuuga agucguggag     2640

aagaugaagg cuuuuuggca gcagcuguug gacuccaagc ucaucucaga aaggaaauau     2700

aacaaucuga ccaaggccga gcgcgagaga gggggacuaa acgaacugga uaagguuggc     2760

uucauaaaac ggcagcucgu ggagacuagg caaaucacca agcauguagc acagauucug     2820

gacgcccgau uuaacaaaga agucacagag aagaauaaaa agaaccgcaa ugugaagauc     2880

auuacccuua aaagcaacau gguuucuaau uuccggaagg aauuugguuu guacaaagug     2940

agagagauca acgauuauca ccacgcucau gacgccuacc ugaaugcagu cguggcuaag     3000

gccauacuca aaaaguaucc aaaguuagag ccugaguucg uauacgggga uuaucagaaa     3060

uacgaccuga agagguacau uucccguagu aaagauccca aggaagugga aaaagcgacu     3120

gagaaguacu uuuucuauag caaccuucug aacuuuuuca aagaagaggu ucacuaugcc     3180

gacggcacaa ucgucaagcg cgagaauauc gaauacucua aggauacggg agagauugcu     3240

uggaauaaag aaaaggacuu ugcaaccauc aaaaaggugc uaucccuccc acaggugaac     3300

auagucaaaa agacugagga acaaacaguu ggccagaaug ggggacuguu cgacaacaau     3360

auugugucaa agaaaaaggu gguagaugcc aguaaauuga uccccauuaa gagcggucug     3420

ucgccugaga aauauggcgg guacgcucgg ccaaccaucg ccuauuccgu cuuagugaca     3480

uacaagcaga acaacaagau caaaaauacu augguuggaa uaccggugcu caccaagcug     3540

gaguucgaaa gagaccccaa ccuuuaccug aggaauuugg gcauuaacaa ugucucucau     3600

gugaucaaac ucguuaagua uacggugcug gaguuuaaac acaaguacgg uaccuaucga     3660

cgguucauug uaagcaacca agaacuaaag cgcgcaaauc agaucuuugu cacaacuaaa     3720

cagaugaagc ugauaucaca guacaccaca cuugauaaaa acgagcacga cagucuccug     3780

aagaauaacc aacaguugau ucaguccauc uggaaucagu uccuggaguu uauguuagcg     3840

acugaucucg ugaacaaaaa gcaauaugac gaacugaucc uuaccaauaa ccagacgauu     3900

gaggaaugua gaaauaucau gaacagggug auaacaaccc ugucuuucac uaauauuaac     3960

gccgggcaga aaaagaucau uuuugaugac acauugagcc ucccuaucaa gcgguggcag     4020

uucaccgauu ccgacaaaau ggucaagaaa auaauugagu caacucugau ccaucaaagc     4080

aucaccggcc uauacgaaac acgaauugau cugaguaaac uuggagagga cuga           4134


<210>  3188
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3188
auggagagca accacgacgg cgaacaggug aaguacaccc uggggcucga uaucggaaca       60

uccucuauug guuaugccgc uauagacaaa caucaaaagc ccaucagagc aaaaggcaag      120

cacgucauug ggguuaggcu uuuccaggag ggacagacug ccgcggaucg gcgcgcuuuu      180

cgaacgaccc guagaaggcu gaaacggcgc aaguggagau ugaauuuacu gaacaggcua      240

uucgacccuu accucucaga aguggaucca aauuuucugc cgcggcuucg ccagaguaac      300

cugagcccca aggacgauaa uaaacauuuc aacggcucca cauuguuucc ugacaagacu      360

gaugccgcau ucuaucgaca auacccaacc auguaucacc ucagauacgc ccugaugaca      420

gagaaaagga aguuugacau ccgggaagua uaucuggcua uucaucacau cgugaaauac      480

cgugguaauu uccucaacuc ugcaaccguc gauucguuua agacuucaaa uauagacuuc      540

acaagccagu uugaucgcuu aaacgagcug uacagacagg ugauccuuga cgagcccuuc      600

cagauuaaua uggaccaagu ugaugaaaug accaacaagu ugcuggacaa ugaugcccua      660

aaacucgaca cgcagaaaca gguggcuaaa cugcuuccug ucaucuauaa cgauaagcca      720

auugacaaac aguacacuag gaucgccacc gaguuuagua aggcgauacu gggguauaaa      780

acaaaguugg augugauucu caaucuggac acucaaaacg caaaggauug gguaauccgg      840

uuagacgaug aagacauuga cgauaaacug cccgcccucg uugagaaccu ugacgagucc      900

cgacaggaaa ucgugaccau cauucgcgau cuguacgcuc agauaacauu gaaugccauc      960

gucccuaagg gaaaaucucu aagcgagucc augguggaca aguauaacga ucacaaagac     1020

caucuggauc uccugauggg ccuuauucac gaacugggcg acgauucagc aaaggggauc     1080

aaguugagag aggcuuacac ccaauaugug ggaaaaagug acgauaagac gcucaaucag     1140

gacgauuucu acgccgcgau aaagaagaac cuggacgaag acagcaaaag gucuccaaag     1200

auucagcggu uaaucgagca ggccuccuuc augccgaagc aacgcacuuc agcaaauggu     1260

gucauccccc aucagcugca ccagcuagag cucgauagaa uuaucgaaaa ccagggcagg     1320

uacuauccuu uucugaaaaa gccaaauccc aacacacaca aaccuaaugg gggaaaguac     1380

aaacuugacg aguugguugc uuuccguauu ccauauuacg ugggcccccu gauaaccaag     1440

cgggaucaag aaaagacuag cggugccgac uuugcuugga ugauccgaaa accggggcag     1500

gagggagaaa uuacaccuug gaacuucgau cagaagguag acagaauggc cagugcaaau     1560

agguuuaucc gccggaugac cacgaaagau ucuuaucucg uggccgagga cguccugccc     1620

gaugcuuccc uuaucuacga gaaguucaaa gcgcugaacg aauugaauau gcucaaggug     1680

aacgacagca gacugucgcc aucucagaaa caagauuuau uuaauaaccu guucaagaag     1740

cagaaaacca uuaggacaaa gaaacuacag acuuauauac gcaccaacug ggagcucccu     1800

uccguuauga ucucaggccu ggcagacccc gauaaguuua auaguagccu uuccacauac     1860

auugacuucc gagacaucuu uggcgaaaaa guggaugacc caaaccggca ggccgauuac     1920

gaggaaauua ucgagugguc uacugucuuc gaggaccgua agauauauca agcuaaguug     1980

aaucagcugg auuggcucac cgaaccccag agaaaagccc uguuaacaaa gagguacacc     2040

ggguggggac gccugagcaa aaaguuauug acuggucuca aagaccaaaa cggcgauuca     2100

auccuggacc agcuauggaa gacgaaugau aacuuuaugc agauucagag ucgggacgag     2160

uucgcaaagc aaauccauga ugaaaaugcc aaacaguucc acgaggcuaa caauguggac     2220

gacauucugg augacgccuu uacauccccu cagaacaaga aagcaaucag acagguagau     2280

aagguugugc aagacgucgu gaaagcgguc ggguaugcuc cagauaagau agccauugag     2340

uucaccagga gccucgaaaa uaacccgcag cgaacugugu cucgccagcg gcagcugcaa     2400

gcagccuaca agaauauccc cagagagauu augaaaucag gacuuauggc ugaauugggc     2460

aguguugccg acagcaagaa agugcugucc gauaaguuau aucucuacuu uacccagaug     2520

gguaaagaca uguaugagga uaagacaauc aacauagaca aucugccuaa cuacgauauc     2580

gaccauauuc uuccacaggc auucaucaaa gaugacucua uugacaacag gguacugauc     2640

ucgaaggaag agaaugcucg uaagagcaac cgcguccccu ugaaauuuuu cggggccaag     2700

cggaugggcu uuuggaaaga ucuccugaau aacggacuaa uauccaauag aaaguuccaa     2760

aaccugacca cugacccuga gaaaauuuca aaguacagug cgaagggcuu uauccacagg     2820

cagcucgugg aaacaucuca gguuauccga cugguggcca auauucuugg gaacgaguau     2880

agccaggcug gaacgaccau cauugaaguc uccgcaaaaa ugaaucggca auugcgcgag     2940

gauuuccauc ugauaaagaa cagaaaugug aacgacuacc accacgccau ggaugcuuau     3000

uuaacugccu acguagguga cuaucucuac cugagguauc caaaacuucg gcccuacuuu     3060

guguacggcg auuucaagaa agcagaagac caggaucuga auauccgaaa cuuuaauuuc     3120

cuacaugacc ucaaggauaa cgagucacug gacaagauug uugauagcga gacaggggaa     3180

aucgucuggg agaaacacgg aucugugaag caguugaaaa agguguauca uuacaaauuc     3240

augcuggucu cccaggaagu uuauaccaga caagacgcgu uauuuaacca gacaauuuac     3300

ccggccagug acgcugauaa gcgcaagcuc aucccuauaa agaauagcaa gccaguggac     3360

guguauggcg guuacucugg gaacaucgau gccuauaugg caauuguaag gguccacggc     3420

aaaaaggagg acaauuacaa agugguugga auccccaugc gugcugugac uaagcugaag     3480

aaagccgaaa aggagggcca cgauuccuac cuuaacgcag uccaugaggu gcugaaaccu     3540

caguucacca agaaaaagaa aaaucggaag acgggggaaa uuaccgagac aguugacgaa     3600

uuugaugugu ugcucggaaa gguauauuac agacagcuga ucgucgacgg uaacaaaaag     3660

uucaugcuag gcucaaguag cuaucaauac aaugccaaac agcuggugcu uucggauaag     3720

gcuaugcaag ugcucuccaa aaacgggaag gucaagucuc aggacgagaa ucagaaccug     3780

auagauguuu augacgagau uuuggaaaaa guggaccagu acuuugagcu guaugaucag     3840

aggcacuacc gcgaaagccu ucauaaggga cgaggcaucu ucguaaaucu cccccuguca     3900

aaccaauacg acgguaguaa acuuacuucc uaugggaagu acgagaccau cgaugcgauu     3960

cugaauggcu ugcacgccaa cgcaacaaug ucuaaucuca gauaucuggg aauaagcucc     4020

ccauuuggca aaaugcaggc uucaaacggg cuaacuaucg acccugauag cauuaucugc     4080

uaccagaguc ccaccggacu guucgaacgg cagauuaaac uuucugaccu cuga           4134


<210>  3189
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3189
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acuaugcaag aggaaaagua cuaucaugag aaauucccga ccaucuacca ccuucgcaag      420

gaacuggcag acaagaaaga gaaggccgau uugcgacucg uguaccuggc ucuggcacau      480

auuaucaaau uuagagguca cuuccucauu gaggacgaua gguuugacgu ccggaacaca      540

gacauccaga agcaguauca ggccuucuua gaaauauuug auaccacuuu cgagaauaac      600

caucugcuuu cucaaaaugu ggacguugaa gcuauuuuga cagauaaaau cucgaaguca      660

gccaaaaagg accguauucu ggcgcaguac cccaaccaaa agagcaccgg gaucuuugca      720

gaguuccuaa aacucaucgu gggaaaucag gccgauuuca agaaacacuu uaaccuggaa      780

gacaagacgc cucuucaauu cgcuaaagau aguuaugacg aggaucugga gaauuugcuc      840

ggccagauug gcgacgaauu ugccgaucug uucuccgucg caaagaaguu auacgacucu      900

gugcugcuca gcgggauacu uacuguaacc gaccugucca caaaagcucc auugucagcc      960

aguaugaucc agcgcuauga ugagcaccau gaagaccuaa agcaccugaa acaguuuguu     1020

aaggcgagcc uccccgagaa cuacagagaa guguucgccg auucuuccaa agacggauau     1080

gcagguuaca uugagggcaa gacuaaucaa gaggcuuuuu acaaguaucu gcuuaaacug     1140

uugaccaagc aggaaggguc agaguacuuc cucgaaaaga ucaagaacga ggauuuucug     1200

aggaaacagc ggacauucga caacggaagc auaccucauc aaguccacuu aaccgaacug     1260

cgagccauua uccgcagaca gagugaguau uacccauuuc uaaaggagaa ucaggauagg     1320

aucgaaaaga uucucacguu ccggaucccc uauuacgugg gcccucuggc ucgcgagaaa     1380

ucugacuuug ccuggaugac uagaaagaca gaugacucca uuaggccaug gaacuucgaa     1440

gaucuugugg acaaagaaaa gagcgcagag gccuucauac accguaugac caauaacgau     1500

uuguaucugc cggaagagaa aguccucccc aagcauucgc ugaucuacga aaaauuuacu     1560

guuuacaaug agcuuacaaa ggugcgguuc cuggcugaag guuuuaagga cuuccaguuu     1620

uugaaccgaa aacaaaagga gaccauuuuc aauucucucu uuaaagagaa gagaaaagua     1680

acggaaaagg acaucaucuc cuuccugaac aagguggaug gguaugaggg aauugcgaua     1740

aaaggcaucg aaaagcaguu uaaugcauca uuaaguaccu accacgaccu gaaaaagauu     1800

cuaggcaaag auuuccucga caacacagau aaugagcuga uccuugaaga cauuguccag     1860

acuuugaccc uguuugagga uagggagaug aucaagaagu gccucgacau auauaaagau     1920

uucuuuacag aaagccagcu gaagaaauua uaccgccggc auuauacugg guggggaaga     1980

cuguccgcca aacuuaucaa cgguauuagg aauaaagaga accaaaagac caucuuggac     2040

uaccucauug augacggcuc ugcuaaucgc aacuucaugc agcugaucaa cgaugacgac     2100

cugagcuuca agccuauaau ugauaaagcc cgaacagggu cacacaguga caaucuaaag     2160

gaagugguug gagagcucgc aggcucccca gccaucaaaa aggguauucu gcagagccuu     2220

aaaaucgugg augaauuggu caaggugaug ggguaugagc ccgagcagau aguaguugaa     2280

auggcucggg agaaccaaac cacugccaaa ggccugucuc guucaagaca gagguuaacg     2340

acacuccgcg aaagucuggc aaaucuuaag agcaacaucc uggaggagaa gaaaccuaag     2400

uacgugaaag accaggucga aaaucaucac uuguccgaug accggcucuu ucuguacuau     2460

cuacagaacg gaagagauau guacaccaag aaagcgcugg acauugauaa ucucucucaa     2520

uaugacaucg aucacauuau cccacaggcu uucauaaagg acgacucgau ugauaacagg     2580

gugcugguca gcuccgccaa gaaucgaggc aaaucagacg augugcccag uaucgagauc     2640

guuaaggcac gcaaaauguu uuggaagaac cuuuuggacg ccaaacugau gucucagcgg     2700

aaguacgaua auuuaacuaa ggcugaaaga gggggacuca ccagcgacga uaaagccagg     2760

uucauucaac gucagcuggu ggagacacgc cagaucacca agcauguagc acggauucuu     2820

gacgaaagau uuaacaauga ggucgauaac gguaaaaaga uauguaaagu gaagaucguu     2880

acucugaagu ccaaucuagu gucaaacuuc aggaaagagu uuggcuucua uaagauucga     2940

gaagucaacg acuaccacca ugcucacgau gccuaucuca augcgguggu agccaaagcu     3000

auccugacaa aguacccgca guuggagccu gaauuugugu acgggaugua ucggcagaaa     3060

aagcugagca agauuguuca cgaggacaaa gaagagaagu acucugaggc aacgcgcaaa     3120

auguucuuuu auuccaacuu aaugaauaug uucaagagag ucgugaggcu cgccgaugga     3180

aguaucgugg uccggcccgu uauagaaacc ggccgauaca ugagaaaaac ugcuugggac     3240

aagaaaaagc auuucgccac agugcgcaag gugcugagcu auccacagaa caauaucguc     3300

aaaaagaccg agauucagac agguggguuu ucuaaagaau ccauccuugc acacggcaac     3360

ucagacaagc ugauuccuag gaaaacuaag gauaucuacu uggaccccaa gaaauaugga     3420

ggcuucgaua guccaauagu agcguacagc gugcucguug uggccgacau uaagaaaggg     3480

aaggcucaga aacugaagac cgucacggag cuacugggaa ucaccaucau ggaacguucg     3540

cgguuugaga agaaucccuc cgccuuccuu gagucuaaag guuaccucaa cauuagagau     3600

gacaagcuga ugauauugcc uaaauauagc cuguuugaau uagagaaugg caggcgccga     3660

cuccuggcau cagcugggga acuucaaaag ggaaacgagc uggccuugcc aacacaguuc     3720

augaaauuuc ucuaccuggc aaguagauau aaugagucca agggcaagcc ggaagagauc     3780

gagaaaaagc aggaguucgu gaaccagcau guuucuuacu uugaugacau ucuacaacug     3840

aucaaugauu ucagcaaacg ggugauucuu gccgacgcua accucgaaaa gaucaauaaa     3900

cuguaucagg auaacaagga gaacauaccc guagacgagu uggcgaauaa caucauuaau     3960

uuauuuacuu ucaccucccu gggugccccu gcagcuuuua aauucuucga uaagaucguc     4020

gacaggaagc gcuacacauc aacuaaagaa gugcucaaca gcacccugau ucaccagagu     4080

aucacggggc uuuaugagac acguauagac cugggcaagu ugggagaaga uuga           4134


<210>  3190
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3190
auggagaaga ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

auaacugaug acuauaaagu ucccgcuaag aaaaugaagg ugcucgguaa uacggauaaa      120

aaguacauca agaaaaaccu ucugggcgca uuguuauucg acucugggga aaccgccgag      180

gcgacaagac ugaagagggc ugcccggcgc cgauauacuc guaggaaaaa uaggcuacgg      240

uaccuccagg aaauuuuugc aaaggagaug gccaaaguag augaaucauu cuuucaacgc      300

cuggaggaga guuuccuuac cgacgaugac aagacauuug auagccaccc uaucuucgga      360

aacaaggcug aagaggacac cuaucaucag gaauuuccaa cuauuuacca ccugagaaaa      420

cauuuggcag auuccccgga gaagguggac cucaggcugg ucuaucuggc ccucgcucac      480

augaucaaau uccggggcca cuuuuuaaua gagggucagc ugaaugccga aaacacagau      540

gugcagaaga ucuucgcgga cuuuguuggg guguacgacc gcaccuucga ugacucucac      600

cuuucggaga uuacggucga ugcagccuca aucuugacug aaaagauuag caagagucga      660

agacuggaga aucuaaucaa acaauacccc accgaaaaga agaacacacu cuuuggaaau      720

cugguggcuc uugcccuggg cuugcagccu aacuucaaaa cuaauuucaa gcucuccgag      780

gacgcaaaac ugcaguuuuc uaaggauacc uaugacgagg auuuagagga gcugcucggc      840

aaaauagggg acgauuacgc ugaccuuuuc acagccgcga agaaccugua ugaugccauu      900

uugcuaagcg gaauccugac cguagacgac aauuccacga aggcaccacu cucagcuagu      960

augauuaaaa gguacgagga gcaccaugaa gaucuggaga aacuuaaaac uuuuaucaag     1020

guuaacaauu ucgacaaaua ucacgagauc uuuaaggaua agagcaaaaa cgguuacgcc     1080

ggcuauauug aaaacggggu gaagcaggac auauucuaca aacaucugaa gucuaucauu     1140

uccgagaaaa auggaggcca auacuuuuug gauaagaucg aacgggagga cuuccuccgu     1200

aagcagcgca cauuugauaa cgguucaaua cccuaucaga uucaucugca ggaaaugaga     1260

accaucuuaa ggcggcaagg ggaguacuau ccuuuccuga aagagaauca ggcuaagauc     1320

gagaaaauuc uaacuuuucg aaucccauac uaugucggac cccucgcccg caagaacagc     1380

agauucgcau gggccaaaua ccacagugac gaaccuauua caccauggaa uuuugaugaa     1440

gugguggaca aggagaaauc ugcugagaag uucauaacca ggaugacgcu gaacgaucuu     1500

uauuugccgg aagagaaggu ccugcccaaa cauuccuacg uuuacgaaac cuucgcggug     1560

uauaaugagc ucacaaagau caaauacgua aacgaacagg gcaagagcuu uuucuuugac     1620

gcaaauauga aacaagagau uuucgaucac guguuuaagg agaaccggaa ggucacuaaa     1680

gccaagcugc uuucguaucu gaauaacgag uucgaagaau uucgcaucaa ugacuugauc     1740

ggccucgaca aagauucuaa guccuucaac gcuucacugg ggaccuacca ugaccugaaa     1800

aagauuuuag auaagaguuu ucuagacgau aaaacaaaug agcagauaau cgaagacauu     1860

gugcucacuc ugacccuuuu cgaggauaga gacaugaucc acgagagguu gcagaaguau     1920

agcgauauuu uuacauccca gcaacugaaa aagcucgaac gucggcauua caccggaugg     1980

ggucgacugu cuuauaaauu aaucaacggc auaagaaaca aggagaauaa caagacuauc     2040

cuggacuucc uuauugauga cggggaugcc aauaggaacu uuaugcaguu gaucaaugac     2100

gacagccucu cauucaaaac gacaauucag gaagcacagg uugugggaga ugucgacgau     2160

aucgaggccg ugguacacga ccugccuggc aguccagcua uaaagaaagg uauucuacaa     2220

uccguuaaga ucguggauga acuggucaaa gugauggggc acaaccccca gaauauuguc     2280

aucgagaugg cccgcgagaa ccagauaacc ggcuacggac ggaauagaag caaccagagg     2340

cucaagcgcc ugcaagacuc ucuuaaagag uucggcucag auaucuugag uaagaagaaa     2400

ccuagcuacg uggacuccaa gguugaaaau ucucaucugc agaacgaucg auuauuucuc     2460

uauuacauuc agaaugggaa agacauguau acuggagagg aacuggauau cgaccaccuu     2520

ucggacuacg auauugacca uaucauacca caggcauuca uuaaggauaa cagcaucgac     2580

aaucgggugc ugaccuccuc agcgaaaaac cgugguaaga gugaugacgu acccucuauc     2640

gaaauuguca gaaacagaaa gagcuauugg uacaaauugu auaaguccgg ccucaucuca     2700

aaacgcaagu uugauaaucu gacaaaagcu gaaagagggg gacuaaccga gacugacaag     2760

gccggcuuca uuaagcggca acugguggaa acaaggcaga uaacgaaaca cguugcacag     2820

auccucgaug cccgauuuaa caccaagugc gacgagaaug auaaagugau ucgcgacguc     2880

aaggugauca cucugaaaag cucucuugua ucccaauucc ggaaggaauu uaaguucuac     2940

aaagugagag agauuaacga uuaccaccau gcucacgacg ccuauuugaa ugcaguuguc     3000

gguacagcuc uguuaaagaa auacccgaag cucgccccug aguuugugua uggggaauac     3060

aaaaaguaug acgugaggaa gcuggucgcg aaaaguagcg auaaccauuc ugagcuuggc     3120

aaggccaccg cuaaauacuu cuuuuauucc aaucugauga acuucuucaa gacagaaguu     3180

aaauacgcag acggacgugu guuugagcgc cccgauaucg aaacuaaugc cgacggcgag     3240

gugguaugga acaagcagcg ggauuucaau auagucagaa aagugcuauc auacccacag     3300

guuaacaucg ugaagaaggu cgaggugcag accgggggau uuaguaaaga aagcauucuc     3360

ccuaagggug acucggauaa acugaucccc aggaagacga aaaaguugca augggagacc     3420

cagaaguaug gcggguucga cuccccaaca guugcuuacu cugugcuggu agucgccgau     3480

guggaaaaag gaaagacucg aaaauuaaag accgugaaag agcucgucgg cauuagcauc     3540

auggaacggu caaguuuuga ggagaauccc guuuccuucc uggaaaagaa ggguuaucac     3600

aacgugcagg aggacaaacu uauaaagcug ccuaaauacu cuuuguuuga guucgaaggg     3660

ggccgcagaa ggcuccuggc aagcgcgaca gaacuacaga agggaaauga aguagugcug     3720

ccacaauaua uggucaaccu ucucuaccau ucccagcacg ugaacaauuc acacaaaccg     3780

gagcaucuga acuauguuaa gcagcacaag gaugaauuua aagacauuuu caauuugauc     3840

aucagcauug cccggauaaa cauccugaag cccaaagugg ucgauaauuu aauuaacgag     3900

uuuacugaau acggccagga ggacaucagu ucucucuccg agagcuucau uaaucugcuu     3960

aaguuuaucu cguucggggc uccuggagcc uucaaauuuc ugaaguugga cgugaaacaa     4020

ucaaaccucc gauacaaguc uaccacggaa gcacugagug cuacacuaau acaucagucc     4080

guuaccggcc uguaugagac uagaaucgau cuuagcaagc ucggugaaga guga           4134


<210>  3191
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3191
augaagaccu ucgccgagga auacgugaug gacaaaagca agcccuaugg caucgggcug       60

gauauuggaa caaacuccgu cgguuuuguu gcuacugacg cagagggcca ccucauaaga      120

cuuaaaggga agacggugau cggagccuac cuguucaaug cgggcauuuc ugcugccgaa      180

aggcgggguu uucgcaccac acgacguaga uugucaaggg uaaaauggcg guuagggcug      240

cuacgcgaga ucuucgaaac ucauuuucag gagaguaugg gagagaacga agauaaugac      300

uucuuucuca gauucaagua uagcaacauu uccccuaagg auccacaauu uucuaccgca      360

aaaggccugu ucaaugacag gacagauaag gaguuuuacg accaguaucc gaccaucuac      420

caccuucggc gcgcccugau gacugaagau caucaguucg acauacgaga gaucuauauu      480

gcuaugcacc auaucgugaa auacagaggc cacuuuuuga aggaggggag ggcaaaagau      540

uucaaggucg gagaccuccg gcugcuggau aacuucaaga ugaugaauga acagauugag      600

gaaaucaacc cccucuggca auuaaaacug ccuacagacg acgccucgau aaagucaauu      660

accgcuaucc uuuuggauaa uacgcagagc cagaacgacc gucagaaagc cgugacuaag      720

guuauucugg cgacccuagu gaaagcaagu gauaaggaca ucaaugccgc ucgcaaaaga      780

uuugucggug agcucuccaa ggccauggug ggccugaaga caaaacuuug gguacuggca      840

gauguuucuc aaaacgggga cugggaaauc aaguacgaga auuaugcuga uuucgccgag      900

acuauuggaa gcggcgaauc cgacaccaua cagucauugu uuaacgagau caaugaucuc      960

uacgguguga uuacacuggc ggggaucaua ccaaaagaag ccgagaguuu cagcgacgga     1020

auggucagga aguaugaaca ccaucggaaa aacuuagagc ugcucaaggu guacugcgca     1080

gagcagucug auggcaagcg aggccgccag auuagacaaa ccuaugacaa auacaucgac     1140

gggguggauu ccaagcaguu uacgcaggaa gacuucuaua aagcucuuuc aaaguuuacu     1200

gccaaagaug aggcuacaag cgaaaacgcc aagcugaucg cacaagagau ugccgucgga     1260

accuucaugc ccaaguugag gacuaaagcu aaugguacaa ucccucacca gcuacaucag     1320

aaggaacugg acgcgauuau agagaaccag aaaaaguacu acccauggcu cggcgagguu     1380

aaucccgugg aaagucaccg gcgcgcacug ccuuauaaac uugaugagcu gguaucuuuu     1440

agaaucccau acuauguggg gccgaugguc acccccacga agggagaccc ugaaaagucc     1500

aaauucgccu ggauggugag gaaggagcca ggcaccauua cacccuggaa cuuugaucaa     1560

aaaguugacc guagcgcuuc gggugaggcc uucauccagc ggaugaagac uaccgauaca     1620

uuuuugaucg gggaagacgu gcucccucag cagucucugu uauaccaaaa auucgagguc     1680

cugaaugaac uaaacaagau uaugauaaau ggcaaaccaa ucugucgaga gcagaagcag     1740

agacucuuca agcagcuguu uaugcaauau aaaacuguga ccguaaagaa aguucagcag     1800

aaccuuauug caaauggaga agaguccgag aacgugccca ucacaggcuu gucagauccg     1860

cugagguuca auaguagcuu uuccaccuac auugacuaua aggauauccu cgggacugcc     1920

gcugucaacg acaaugccaa acagucugac auagaacaaa ucauugcaug gagcacgauc     1980

uucgaggaug cggcuauuuu ucgcgaaaag cugaacgaca ucacauggcu uaaugaugac     2040

cagcggaaca agcugucaca caaaagauac aggggauggg gucgccauag ucgaaaguug     2100

cucgccggcc ugcgggaugg ggagggacag accauaauug aacguuuaug gaacacuaau     2160

gacaacuuca ugcaaaucca gaaugauucc gagauugcaa gacagaucac cgaggccaac     2220

agcucuaaaa uggcuacagc cgaaggcacc gacgagauaa ucgaugguuu uuacacuuca     2280

ccugaaaaua agaaagcacu gagggaggug augaaggucg ugaaggacau ucagcgcgcu     2340

caccaugggc aagcccccgc guggguuuau aucgaaaguc cacgggagac accuagaccc     2400

ggccagagga cggugagccg agagcagcag cuaaccgauc ucuacgaagg agccgcuaaa     2460

gagauuguag acgaugcagu ccugaacgaa cuuaaggaca aagugaaguc caaagagaau     2520

uucacugaca aguugguucu guauuuucuc caaaacggcc acgauaucua cgccaaugac     2580

ucuauaaaca uugauaaucu gaacgcuuau gacaucgauc auguguuacc acagucgcug     2640

aucaaggacg auagccuuga caaucgcguc uugacaaccc acgagcggaa ccucaaaaag     2700

uccaauagau ucgccacaga acuguuugca gaucagagga aaaaguggga gaaauggcac     2760

cgucuagggc ugauuucaag ucgcaagcuc aaacaucuga cuaugcagcc caacucugug     2820

gaaaaguucg cgcacggauu uaucgcccgg caacuuaccg agacgagaca gauuauacau     2880

uugaccgcua acguacugag caauuuauac caggaaaacg acacaaagau cgugaugauu     2940

aaagccgguc ucaauuccga guucaggcga acuuucgauu uuccuaagaa ccggucaguu     3000

aaugacuauc accacgcauu cgacgcuuuu cugaccgcca aaaucggccg cuaccuucug     3060

gcaagauacc caaaguugga gccguucuuu gucuauggga acuucgugaa aaaucccaag     3120

gccaugaaga ggcucagcuc uuuugauuuc auugcucaac uggcggccaa aacagacgau     3180

acuucccaua ucgaccagcg gagucuaaaa caggugccug ucguuaacga agagaccgga     3240

gaaauagugu gggauaagga caucgagcug gcagaacucg auaagacgua caauuauaaa     3300

acaaugcugg ugaagcgagc ucagaccgag aacaaugccc aaauguuuaa gcagacugua     3360

uucaaagcca gagacaacca gaauaagaca cuuauuccag ucaaaaacgg cuugagcacc     3420

gauguguacg gugggcacuc ucagcaagcu aucuccuauc ugugcauugu uugggugggc     3480

cagaagaaaa aguaccgcgu cuuaggaauc ucaacugcac augccggcau acucaauaac     3540

uuugagaaga acuaugggag guucgaagcg aaaaagaaac ugcaggagau ugugaguaau     3600

acccuugaca acgcugaucg uaaugacuuc aagaucguug cccccaaagu gcuguuugaa     3660

cagguagucg aggaugacaa caugaaguuc ggacuaggca gcgcaucgga cuaccggaau     3720

gugcaacagc ucuuucuguc cagaaagaac caguugcugu uagccaauau gaugacagau     3780

cagauccacg accaagaucu cgugcaucug uucgacgaaa uugucgguca gaugaacgcu     3840

cacuuuccua uauucgaucg cgggggauac aggucuagcc uuacgcaguc acgagacaaa     3900

uuucugaagu ugccauucaa aaagaaugag gaucucauca ccaaacaaga gguuauuaga     3960

cggauccugg acggccuaca cgcaaacgcc aauaggaagg aucugaaaau uaucgguagu     4020

aagggcgacu uugggcgccu uggaacuaag aaaauauauc ucuccaagga ugcgaaacug     4080

aucuacacau cucccaccug uuuguucaca cguacugugc cgcugagcuc cuuauga        4137


<210>  3192
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3192
augaccaacg agaaguacag caucggccug gacauuggga cauccucuau aggauucgcc       60

guggucaaug auaacaauag aguuaucagg gugaaaggua agaacgcuau uggcguacgg      120

cucuuugacg aagggaaagc agccgcggau cgccgaucau uccguacuac gagaaggcgg      180

cuuagucgca gaagguggcg gcugaaguug uuacgcgaga ucuuugacgc uuauauuacc      240

cccguggaug aagccuucuu uauccgacug aaagagagca aucuaucccc uaaggacucu      300

aagaaacagu acucgggaga uauacucuuc aacgacagau cagauaagga cuuuuaugaa      360

aaauacccaa caaucuauca ccugaggaau gcacuuauga cugagcaucg gaaguucgau      420

guccgugaga uuuaccuggc cauccaccau auuaugaaau uucgcggcca cuucuugaac      480

gcuaccccgg caaauaacuu uaaggugggu agacucaauc uggaagagaa guucgaagaa      540

cugaacgaca ucuaucaaag gguuuuuccc gaugagagca uagaguuccg gacagacaau      600

cucgagcaga uuaaagaagu guuacuggac aacaagcgaa gucgcgccga uagacagagg      660

acccuugucu ccgacaucua ccagucuagc gaggauaaag acauugaaaa gcggaauaaa      720

gcuguggcca cugagaucuu gaaggcgucc cuggggaaca aagcaaagcu aaacguaauc      780

acaaauguug agguggauaa ggaagccgcu aaagaguggu caauuaccuu cgacagugaa      840

agcauagaug acgaucucgc caagaucgag ggacaaauga cggacgaugg ccaugaaauu      900

aucgaggucc ugcgcucucu uuacuccggc auaacucugu cagcaauugu gccugagaac      960

cacaccuuga gccagaguau gguggcuaaa uaugaccucc acaaggacca ucugaaauua     1020

uuuaagaagc ugaucaaugg gaugacagau acuaaaaagg ccaaaaaccu cagagcggcc     1080

uacgacggau auaucgaugg ugucaagggc aaaguucuuc cacaggaaga cuucuacaaa     1140

caggugcaag uaaaucugga ugacucugca gaggcuaacg aaauucagac cuauaucgau     1200

caggacauuu uuaugcccaa gcagaggaca aaagccaaug gguccauacc ucaccaauug     1260

cagcagcaag agcuagauca gaucauugaa aaccagaagg cuuacuaucc auggcuggcc     1320

gagcucaauc ccaacccuga caaaaagcgu cagcaacugg caaaauacaa acuugaugag     1380

cuggugaccu uccggguccc auacuaugug ggaccgauga ucacggccaa ggaccagaaa     1440

aaucagagcg gcgcugaauu ugcguggaug auccgaaagg agcccgguaa cauuacuccu     1500

uggaauuucg aucagaaagu ugacagaaug gcaacagcca accaauuuau aaagaggaug     1560

accacuacag acaccuacuu gcucggggaa gaugugcugc cagcucaguc guuacuguau     1620

cagaaauucg agguccuaaa ugagcucaac aagauccgca uugaccauaa acccaucucu     1680

auugaacaga agcaacagau cuuuaacgau cuguucaagc aguuuaaaaa ugugacgaua     1740

aagcaccuuc aggacuacuu gguaucccaa ggacaguauu caaaacggcc ucugaucgag     1800

ggccucgccg augaaaagag auucaacagu agccugucca ccuacucuga ccuuugcggc     1860

auuuuugggg caaaacuggu ugaggaaaau gauaggcagg aggacuugga gaagaucauu     1920

gaauggagca caaucuucga ggauaagaaa auauaucgcg ccaagcucaa cgaccugacu     1980

ugguuaaccg augaccaaaa agaaaagcug gcuacaaaac gauaccaggg auggggucgg     2040

cuaucacgua agcuccuggu gggccuuaag aauagugagc acagaaacau uauggauauc     2100

uuguggauua cuaaugaaaa cuucaugcag auccaggccg agccagacuu ugcaaaacug     2160

gucaccgacg cgaauaaggg gaugcucgag aaaacagauu cccaagacgu gauaaacgau     2220

cuguacacca gcccccagaa uaagaaagcu aucaggcaga uuuuacuggu cgugcaugac     2280

auccagaacg ccaugcacgg acaagcaccg gccaagauuc auguugaguu cgcucgcggc     2340

gaagaacgga auccuagaag gucugugcag cgacagcgcc agguagaggc cgcauaugaa     2400

aaggucucaa acgagcuugu gagugcuaaa guucggcaag aguuuaagga agccaucaau     2460

aacaaaagag auuucaagga cagguugcuc cuguacuuua ugcagggugg gauagauauu     2520

uauacuggca aacagcuaaa caucgaccaa cugagcuccu accagaucga ucacauucuc     2580

ccccaggcgu ucgugaagga cgauucucug acgaaucgug uccuuacaaa cgagaaucag     2640

gugaaagccg acucgguacc aaucgacauu uuuggaaaga agauguugag cguguggggc     2700

cgcaugaaag aucaagggcu gauauccaag ggaaaauauc ggaacuuaac caugaauccu     2760

gaaaacaucu cagcucauac ugagaauggu uucauuaaca gacagcucgu ugagaccagg     2820

caggucauca agcuggcagu gaauauucuu gccgacgaau acggcgauag uacacagauc     2880

auaucuguga aagcugaccu gagccaccaa augcgagagg auuuugaauu gcucaagaac     2940

cgggacguca augauuauca ccaugccuuc gacgcauacc uggcggccuu uaucgggaac     3000

uaccuacuga agcgcuaucc caaacucgag uccuacuucg uuuauggaga uuuuaagaaa     3060

uucacccaga aggaaacuaa aaugagaagg uucaauuuua uuuacgaccu gaagcacugu     3120

gaucaggugg ugaacaagga gacaggcgag auccuuugga cgaaagacga agauauuaag     3180

uauauccggc auuuguucgc uuacaaaaag auacugguau cacacgaggu ccgagaaaag     3240

agaggugccu uauauaauca gaccauuuac aaggcaaagg acgacaaagg gagcggccaa     3300

ggaucuaaga aacucauccg caucaaggau gacaaagaga cuaagauuua cggcggguau     3360

uccggaaaga gucuggcuua caugacaaua gugcagauca ccaaaaagaa caaaguuagc     3420

uauaggguga uugguauccc aacacuugcc cuggcacguc uaaacaagcu cgaaaaugau     3480

ucuacugaga acaauggcga gcuguacaaa auuaucaagc cccaguuuac ccacuauaaa     3540

gucgacaaga agaacgggga aauaaucgag acgaccgaug acuucaaaau ugugguuucc     3600

aaggugcggu uucagcaauu gaucgaugac gccggacagu ucuuuaugcu ggcuucagau     3660

acauacaaaa auaacgcgca gcaauuagua auuaguaaua acgcccucaa ggcaaucaau     3720

aacacuaaua uaaccgacug cccuagagau gaccuggaaa ggcuugauaa ccugcgcuug     3780

gacagcgcuu ucgacgagau ugucaaaaag auggauaagu auuuuucggc cuacgacgcc     3840

aauaacuucc gagaaaagau cagaaauucc aaccucaucu uuuaccagcu gccaguggag     3900

gaucaguggg agaauaacaa gauuacagaa cuaggcaaac ggacugugcu gaccaggauc     3960

cuucaggguc uccaugcuaa cgcaacgaca accgacaugu cuauauucaa gauuaaaacu     4020

ccguucgggc aacugcgcca gcguagcggc aucucauuga gugagaaugc ccagcugauu     4080

uaucaguccc ccacaggauu auuugaaaga cggguccaac ucaacaagau caaguga        4137


<210>  3193
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3193
augaagaaca aagacuacag caucggccug gauauuggga ccuccucuau aggauucgcc       60

guggucgacg agaauaacca cguuaugaga gugaagggua aaaaugcuau cggcguaagg      120

cucuuugaug aagggaagcc cgcagccgac cggcgcgcgu uccgaacaac ucguagaagg      180

cuuucacggc gcagauggag gcugaaauug uuacgggaga uuuuugauag uuauaucacg      240

ccuguggacg aggcuuucuu uauucgccug aaggaaagca accuaucccc aaaggauucu      300

aaaaagcagu acucgggaga cauccucuuc aaugaucgau cagacaaaga uuuuuauacc      360

aaguacccga caauauauca ucugagaaac gcccuuauga cugaacacca aagguucgac      420

auccgggaga uuuaccuggc aauccaucac auugucaaau uucguggcca uuucuugaau      480

gccacccccg cuaacaauuu ucaggugggu aagcucgauc uggaccagaa guucgaggac      540

cugaacgaua ucuaucagcg cguuuuuccu gacgauagca uagaguucag aauugacaau      600

cucgaacaaa ucaaagcagu guuacuggau aguaagaggu cccggucuga ccgacagcgc      660

agccuugucg augacgugua ccagacaucc gaagauaaag agguagagaa gcagaacaaa      720

gccguugcua ccgaaguguu gaaggccauu cuggggaaua aagcgaagcu aaacaucauc      780

gcaaaugucg agguggacaa ggaagccgcu aaagagugga cuauuacauu cgauucagaa      840

aguauagacg acgaucucgc caagaucgac ggacaaauga ccgaugacgg cagagcaauu      900

guggagaucc ugaggagccu uuacucuggc auaacgcugu ccgcuauugu cccagagaac      960

cacacuuugu cacaggccau gguugcgaaa uaugaucucc acaagaaaca ucugaccuua     1020

cugaagaagc ucaucaacag caugacagac acuaaaaagg ccaaaaaucu ucgggcagcu     1080

uacgaugggu auguggacgg aguaaagggu aaagugcuga cccaggauga auuuuacaag     1140

cagauccaaa guaacuugga cgagucuguc gaagccuccg agauucagac auauaucgau     1200

caggacauuu ucaugcccaa gcaacgcacc aaagcuaaug gcagcauacc ucaccagcua     1260

cagcagcaag aacuggauca gaucauugag aaccagaagg aguacuaucc auggcucgcc     1320

gaacugaauc ccaacccuga caaaaagaga cagcaacuug caaaauacaa gcuggacgag     1380

uuggugacgu uuaggguucc auacuaugug gggccgauga ucacugccga agcucagaag     1440

aaacagucgg gagcggaguu cgcauggaug auccguaagg aaucuggcaa uauuacaccc     1500

uggaacuuug aucagaaagu cgaccggaug gccaccgcua aucaauucau aaagcgaaug     1560

acuacaaccg auacguaccu ccugggugag gacguguuac cuucccaguc acugcuauau     1620

cagagauuug agguacucaa cgaacugaau aaaaucagga uugaucgcaa gccaaucagu     1680

guugagcuua aacagcaaau uuucaacgac uuguuuaaga aguucaaaaa ugugaccauc     1740

aagcaucugc aggauuaccu cgucgcccag gggcaauaua gcaaacggcc ccugauagaa     1800

ggacuugcag acgagaagag auuuaacucc ggccugucua cauacaauga uuucugcggc     1860

aucuuugggg ccaaauuggu ggacgagaac gauaggcagg cugaccucga aaagauuauc     1920

gaguggagca cuauuuucga agauaagaaa aucuaucgcg ccaagcugaa cgacuuagca     1980

uggcugaccg acgagcagaa agauaagcua gcgacaaaac gauaccaggg auggggucgg     2040

cucucaagua agcugcuuau aggcuugcaa aauuccgaac accguaacau uauggaccug     2100

cucuggaucg ggaaugagaa cuucaugcag auucaggcug auagcgacuu ugccaagcug     2160

aucaaagaug caaaucagaa cgccuuacaa aauaacgacu cucaggaugu cauaaaugac     2220

cuguacacuu caccucagaa caagaaagcu aucagacaga uucuuuuggu gguucacgau     2280

auccaaaaug ccaugcaugg acaggcacca accaagauuc acguggaguu cgcuaaaggc     2340

gaagagagga acccccgccg gaguguacag agacaaaggc aggucgaagc cgcguaugag     2400

aaggccagca augaacucgu gggugcuaac guucgagagg agcugaagga cgcaaucaca     2460

aauaaacgcg auuuuaagga ccggcuauuc cuguacuuua ugcaaggggg cguggacaug     2520

uauuccggag aaccgauaaa cauugauaac cucucuagau acgacaucga ucauauccug     2580

ccucaggccu ucgucaaaga cgauucgcuu aauaacaggg uguugaccga cgcugagaag     2640

aaucgucgca aaagcgaugc cguacccaac gaacuguuug gcgcaaagau gcgggugacu     2700

ugggagagaa ugaaagaaca gggguuaauu uccaagggaa aguauaggaa ucucacgaug     2760

aacccagaga auaucaacaa auacacagag aaugguuuca uuaaccgaca gcugguugaa     2820

acccggcagg ucauaaaacu ugcggugaau auccuggccg acgaguauaa ggaaaacacu     2880

cagauuaucu cagugaaggc ugauuugagu caccaaaugc gcgaggacuu ugagcuccug     2940

aaaaauagag augucaacga cuaccaucac gccuucgaug cauaccuagc ugccuuuaug     3000

ggcaauuauc ugcucaagag guacccuaag cugcggucuu auuucguuua cggggacuuu     3060

aaaaaguuca cccagaaaga aauuaaacuu cgaagauuca acuuuaucua ugacuugaag     3120

cacgaggauc aggugauaga caaggaaaca ggagagaucc uguggaacaa gaaugaagau     3180

auugcauaca uccgccauuu auucgccuau aaaaagauuc ucaucagcca cgaggugucc     3240

gagaggcgug gcgcucugua caaccagacc guauacaaag cgucagacga uaaggccagc     3300

ggucaagggu cuaaaaagcu gauacccauu aaguccgaca aaccaacuga aaucuauggc     3360

ggauacacag gccggacgcu ugcauauaug accaucguca aggugacuaa agggaagaaa     3420

auuaguuaca agguuguggg aguccccaca gcugugcuag ccaagauggc cgguguuaga     3480

cagggcagcc aggaggaaca ggauaaacuc uauggggugc ugaagccuca auuuacccau     3540

cagaaagaga auaagaaaac aggagaaaua acugagaccg uagacaaguu cgauaucauu     3600

gucccaaaag ugaaguacca gcaauuggug aacgacaaug gccagcuguu uaugaucggu     3660

ucugauacgu uaccguauaa cgcugagcag cucguccugu ccaaggaauc aguucagacc     3720

auuaauaacc ccgggaucaa agacgagggc agugaugaag agagcgaaaa uaagaaacuu     3780

gacaacgugu acgacgagau aguacugugu augaauaagu acuucccuuu guaugauaac     3840

aggggauuuc gcaauucgau cacaaacggc aaaaauaagu uccucaagcu gccaauuaaa     3900

aacaucuaca agaaugggaa acuaauuaac gugggaaagc gagaggucau caacagaaua     3960

cugcaaggcc uucacgcaaa ugccuccgug acugaccucu cuaaccuggg uauuaaaagc     4020

cccuuugggu ucuugcagaa gggagcgggc cugucauuaa guggugaagc ucagcucauc     4080

caccaguccg ccaccggccu guuugagcau cggguucaac uuaauaagau caaauga        4137


<210>  3194
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3194
augagcaaca agcccuacuc caucggccug gacauuggga ccaauucugu gggaugggcc       60

gucauaacag augacuauaa aguuccuuca aagaaaauga aggugcucgg uaacacugau      120

aaacacuuca ucaagaagaa ucuuauuggc gcucuguugu uugacgaggg gacgaccgca      180

gaaggaagaa gguuaaaacg gacagcccgc cgacguuaca cuagaaggaa gaaccggcug      240

cgcuaucuac aggagaucuu cagugaagag auuagcaaag uagauuccuc guuuuuccau      300

agacucgacg auucuuuucu ggugccagaa gacaagaggg gcucaaaaua cccgaucuuc      360

gcgacccuug cugaggagaa ggaauaucac aagaauuuuc ccacaauaua ccaucugcgg      420

aaacaauugg ccgauagcaa ggagaaagca gaccuccgcc ugaucuaucu ggcccucgcu      480

cacaugauga aguaccgagg ucauuucuua uacgaagaga guuuugauau uaaaaacaau      540

gacauccaga agauuuucaa cgaguuuauc uccauauaug auaauaccuu cgaagggucu      600

agccuguccg agcagaacgu ccagguggaa gcaauuuuua cugacaaaau cucaaagagu      660

gccaagagag agaggguucu uaaauuguuc ccugacgaaa agagcacagg acuguucucu      720

gaguuucuaa aacucauugu gggcaaucaa gcugaguuca agaaacacuu ugaucuggaa      780

gagaaggccc cacuucaguu cuccaaggac accuacgaug aagaccugga gaacuugcuc      840

ggccagaucg gggauggauu ugcggaccug uucuuagucg ccaaaaagcu guaugaugca      900

auccuccuuu cagguauucu gacggugacu gaccccagca ccaaagcucc uuugagugcc      960

ucuaugauag aacgguacga gaaucaccag aaggaucuag caucccugaa acaauuuauc     1020

aagaacaauc ucccagcuga gaagugcgua gaaguuuuca gcgaccaguc gaaagauggc     1080

uaugccgggu acauugacgg aaagacaacu caggaggcgu uuuauaaaua caucaagaac     1140

cugcuuucua aacuggaagg cgccuacuau uucuuggaca agauagagcg ugaagauuuu     1200

cuccgcaagc agagaaccuu cgacaacggu uccauucccc aucaaaucca ucugcaggag     1260

augaaugcaa ucuuaaggcg gcagggggag cacuacccuu uucugaaaga aaaccgagag     1320

aagauugaga aaauccuaac auuccgcauu ccauaucacg ugggaccgcu cgcuagaggc     1380

aauagggauu ucgccuggcu gacccggaac ucagacgaag cuauacgccc cuggaauuuu     1440

gaggaaaucg ucgaucaagc caguagcgca gaggacuuca uuaacaagau gacgaauuac     1500

gaucuuuauu ugccugaaga gaaagugcug ccaaagcacu cccuccugua cgaaacuuuu     1560

gccguguaua acgagcuuac aaaagucaag uucaucgcug agggccugac cagauaccag     1620

uuuuuggaca agaaacagaa gaaagauauc uucuacacau uuuucaaggc ggaagacaaa     1680

aggaagguua cugagaagga uauuauacau uaucuccaca auguggacgg guacgaugga     1740

aucgaacuga aagguauuga gaagcaguuu aacgcaucuu uaagcaccua ucaugaccug     1800

cuaaaaauca uuaaggacaa agaguucaug gaugacccca agaaugaaga gauccucgaa     1860

aacauaguac acacgcugac caucuuugag gaucgugaaa ugauuaagca acggcuugcc     1920

caguacgacu cauuguucga ugagaaagug aucaaggcuc ugacacgaag acauuauacu     1980

ggcuggggga aacucagugc caagcugauu aauggaaucu gugacaaaca gaccggcaag     2040

acaauauuag auuaccugau ugacgauggu aagaucaaca ggaauuuuau gcagcuuauu     2100

aacgacgaug gguuguccuu caaagaaauc auacaaaagg cacaggucgu gggcaaaacu     2160

uaugacguua aacagguggu ccaggagcuc ccuggaagcc cagccaucaa aaagggcauu     2220

cugcaaucug ugaagcuagu agaugagcug guuaagguga uggggcacgc ucccgaauca     2280

aucgucauug agauggcccg cgaaaaccag accacagcaa aggguaaaaa gaauagucag     2340

cagcgguaca aaagaaucga ggacgcgaua aagaaccucg cuccgggacu ggacagcacc     2400

auucuuaaag agcacccuac ugauaauauc caauugcaga acgacaggcu guucuuauac     2460

uaucuccaga auggccgcga uauguacacg gggaagcccc uggaaaucaa ccaacuuucc     2520

aauuaugaca uugaucauau cauuccacag gccuuuauaa aggacgauuc ucuggacaac     2580

cgaguguuga caucgagcaa agagaaucgg ggaaaguccg auaacguccc uucacucgaa     2640

gugguugaga aaaugaaggc auucuggcag cagcugcuag acaguaaacu gaucucugaa     2700

cguaaguuua auaaccucac caaggccgag agaggcgguc uggaugagcu ugacaaagug     2760

ggguucauua agaggcaauu gguagaaacu cgccagauca ccaaacacgu cgcucagauu     2820

cuggacgccc gguuuaauaa ggaggugaca gagaaaaaca agaagaauag aaacguuaaa     2880

aucauaaccu uaaagagcaa cauggugucc aauuucagga aagaauuugg ccucuacaag     2940

guccgagaaa ucaacgauua ucaucacgca cacgacgcuu accugaaugc cgugguagcg     3000

aaagccauuc uuaagaagua ucccaaacug gagccagagu ucguguacgg agauuaccag     3060

aaguaugacc uaaaacgcua caucucaaag agcaaagauc ccaaggaagu ugagaaagcu     3120

acugaaaagu acuuuuucua uucuaaccuc cugaacuuuu ucaaggagga aguccauuau     3180

gcagacggca caauugugaa acgggagaau aucgaguacu ccaaggauac gggggaaaua     3240

gccuggaaua aagagaagga cuucgcuacc auuaaaaagg uguugaguuu uccucaaguc     3300

aacaucguua agaaaacuga ggaacagaca gugggacaga augguggccu guucgauaac     3360

aauaucguga gcaaggagaa aguagucgac gccucuaagu uaauuccaau aaaauccggg     3420

cucucaccgg aaaaguaugg aggcuacgca agacccacca ucgcguacag ugugcugguu     3480

acauauaagc agaacaauaa aauuaagaac acuauggugg guaucccugu ccuuaccaaa     3540

cuggaguuug aaagggaucc aaauuuguac cuccguaacc uggggauuaa caaugugagc     3600

cacguuauca agcuagugaa acauacggua cuggaguuca agcacaagua uggcaccuac     3660

cgccgguuua uagucucgaa ccaagaacuu aaaagagcca aucagaucuu cgugacaacu     3720

aagcagauga aacucauuuc ccaauauacc acacuggaca agaacgagca ugauucuuug     3780

cugaaaaaua accagcaguu aauccagagc auuuggaauc aauuucucga guucaugcug     3840

gcuacugacc uugugaacaa gaaacaguac gacgaacuga ucuugaccaa uaaccagacg     3900

auagaggaau gcaggaauau uaugaaccga gucaucacaa cccucucauu uacuaauauc     3960

aacgccggac agaagaagau uaucuucgau gacacacuga gucuacccau aaaacggugg     4020

caauuuaccg auuccgacaa gaugguuaaa aagauuaucg agucuacucu gauucaccag     4080

agcaucaccg gccuuuauga aacacgcaua gaucucaaua aacuggggga ggacuga        4137


<210>  3195
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3195
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uaggauucug      240

uaccuacagg aaaucuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacaga      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acuaugcaag aggaaaagua cuaucaugag aaauucccga ccaucuacca ccuucgcaag      420

gaacuggcag acaagaaaga gaaggccgau uugcgacuca uuuaccuggc ucuggcacau      480

aucauuaaau uuagagguca cuuccucauc gaggacgaua gguuugacgu gcggaacaca      540

gacauacaga agcaguauca ggccuucuua gaaauuuuug auaccacuuu cgagaauaac      600

gaccugcuuu cucaaaaugu cgauguggaa gcuaucuuga cagacaaaau uucgaaguca      660

gccaaaaagg aucguauccu ggcgcgcuac cccaaccaaa agagcaccgg gaucuuugca      720

gaguuccuaa aacucauugu uggaaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacgc cucuucaguu cgcuaaagac aguuaugaug aggaccugga gaauuugcuc      840

ggccagauag gcgaugaauu ugccgaccug uucuccgcag cuaagaaguu auacgacucu      900

gugcugcuca gcgggauccu uacugucauu gaucugucca ccaaagcccc auugucagcg      960

aguaugaucc agagauauga cgagcaccac gaagaucuaa agcaucugaa acaguuugug     1020

aaggccagcc uccccgagaa cuacagggaa guauucgcag acucuuccaa agauggauau     1080

gcugguuaca uagagggcaa gacaucacag gaggccuuuu acaaguaucu gcuuaaacug     1140

uugacuaagc aagaagggag cgaguaccuc cuggaaaaga uuaagaauga ggacuucuua     1200

cggaaacagc gaaccuuuga uaacggaagu aucccucacc agguucaucu gacagaacua     1260

cgcgcuauca uuagaaggca aucugaguau uacccauucc ucaaggagaa ccuggaccgg     1320

aucgaaaaga uucuuaccuu ucgcauaccc uauuacgugg gcccuuuggc cagagagaaa     1380

uccgauuucg cauggaugac gaggaagacu gacgauagca uccguccaug gaauuuugaa     1440

gaccuggucg auaaagagaa gucggccgag gcuuucauuc accggaugac aaacaaugac     1500

cucuaucugc cggaagagaa agugcuuccc aagcacucuc ugaucuacga aaaauucacc     1560

guguacaacg aguugacuaa gguccgauuu cucgcggaag guuucaagga cuuucaguuc     1620

cugaauagaa aacagaagga gacaaucuuu aacuccuuau ucaaagagaa gaggaaaguu     1680

accgaaaagg auauuauauc auuucugaau aagguggacg gguaugaggg aaucgcaauu     1740

aaaggcaucg aaaagcaguu caacgccagu cuaagcacgu accaugaucu caaaaagauu     1800

cugggcaaag acuuucuuga uaauaccgac aacgaguuga uccuggaaga uauaauccaa     1860

acacucacuc uguucgagga ccgcgagaug auuaagaagc gguuagauau cuauaaagac     1920

uuuuucaccg aaucccagcu gaagaaacuu uacagaaggc acuauacagg guggggacgc     1980

uugucugcua agcucauuaa ugguauccga aacaaagaga aucagaagac uauacuggau     2040

uaccuaauug acgauggcag cgccaaccgg aauuuuaugc agcugaucaa ggacgcaggg     2100

cucucauuca aaccuauuau cgacaaggcc cguaccggaa gucauuccga uaaccugaaa     2160

gaaguagugg gcgagcuugc ugguagccca gccauaaaga aagggaucuu gcaaucucug     2220

aagauugucg acgaauuagu gaaaguuaug ggcuaugagc ccgagcagau cguggucgaa     2280

auggcaagag agaaccagac aaccgcgaag ggacucucaa ggagucgcca gcggcugacu     2340

acgcuuagag aaagccuggg caauuugaag uccaacauuc ucgaggagaa aaagccuaaa     2400

uacgugaagg aucaaguaga aaaucaccau cugucugacg auaggcuauu ccuguacuau     2460

cuccagaacg ggaaagacau guacacagau gacgagcugg auaucgacaa ucuuucgcag     2520

uaugauauag accacauuau cccacaggcu uuuaucaagg acgauagcau ugacaaccga     2580

guuuuggugu ccucagccaa gaaucgcgga aaaagugaug acguccccuc uaucgaaauu     2640

gugaaggcac ggaaaauguu cuggaagaac cuguuagaug ccaaacucau gagccaaaga     2700

aaguacgaca aucugaccaa ggcugagagg gguggccuua cuuccgauga caaagcccgu     2760

uuuauacagc gccagcuggu cgaaacccgg caaaucacaa agcacguggc aagaauuuug     2820

gaugagaggu ucaacaauga gcucgacuca aaagggcgac ggauccgcaa gguuaaaauu     2880

gugacccuga aaagcaacuu gguaucuaau uuuagaaagg aguucggauu uuauaaaauc     2940

agggaaguca acaauuacca ucacgcucau gaugccuauc ugaacgcggu gguugccaag     3000

gcuauauuaa cuaaauaccc gcagcucgaa ccugaguucg uguacggccu guaucggcga     3060

aagaaacuuu ccaagaucgu cagagaagac aaggaggaga aauacaguga agcaacacgc     3120

aagauguuuu ucuauagcaa ccugaugaau auguuuaaaa gggugguacg uuuggccgau     3180

gguucuauug ugguucggcc cgucaucgag acggggagau acaugggcaa gaccgcuugg     3240

gacaaaaaga aacacuucgc cacugugagg aaggugcucu ccuauccaca gaacaauauu     3300

gucaagaaaa cagaaaucca gaccggaggc uucucaaagg agaguauacu ggcacacggg     3360

aacagcgaca aacuaauucc ucgcaagaca aaagauaucu accuggaccc caagaaguau     3420

ggagguuuug auucgccaau cguugcguac uccgugcuug ugguagccga cauuaaaaag     3480

ggcaaagcuc aaaagcucaa aacugucacc gaacuguugg ggauaacgau cauggagcga     3540

ucuagauucg agaagaauac cagcgccuuu cuggaaucaa agggauacuu aaacauucgg     3600

gaugacaaac ucaugauccu gcccaaguau agucuuuucg agcuggaaaa uggcaggcgc     3660

cguuugcucg cauccgcugg ugagcugcag aaagggaacg agcuagcccu gccuacacag     3720

uuuaugaagu uccuuuaccu cgcaucuaga uauaaugaaa gcaaaggcaa gccagaggaa     3780

auugagaaga aacaggaauu ugugaaccaa cauguuuccu acuucgauga cauccugcag     3840

uugauaaaug auuuuucaaa ccgggugauc uuagccgacg cuaaucugga gaagauuaac     3900

aaacucuauc aggauaauaa ggagaacauc ccggucgacg aacuggcgaa caauauuauc     3960

aaccuuuuca cuuuuaccag ccugggagcc cccgcagcuu ucaaauuuuu cgauaagaua     4020

guggacagga aacgcuacac aaguacuaag gagguucuaa auucuacccu aauucaccag     4080

uccaucacgg gccuguauga aacacgaauc gaccucggga agcugggaga gggcuga        4137


<210>  3196
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3196
augaccaacg agaaguacag caucggccug gacauuggga cauccucuau aggauucgcc       60

guggucaaug auaacaauag aguuaucagg gugaaaggua agaacgcuau uggcguacgg      120

cucuuugacg aagggaaagc agccgcggau cgccgaucau uccguacuac gagaaggcgg      180

cuuagucgca gaagguggcg gcugaaguug uuacgcgaga ucuuugacgc uuauauuacc      240

cccguggaug aagccuucuu uauccgacug aaagagagca aucuaucccc uaaggacucu      300

aagaaacagu acucgggaga uauacucuuc aacgacagau cagauaagga cuuuuaugaa      360

aaauacccaa caaucuauca ccugaggaau gcacuuauga cugagcaucg gaaguucgau      420

guccgugaga uuuaccuggc cauccaccau auuaugaaau uucgcggcca cuucuugaac      480

gcuaccccgg caaauaacuu uaaggugggu agacucaauc uggaagagaa guucgaagaa      540

cugaacgaca ucuaucaaag gguuuuuccc gaugagagca uagaguuccg gacagacaau      600

cucgagcaga uuaaagaagu guuacuggac aacaagcgaa gucgcgccga uagacagagg      660

acccuugucu ccgacaucua ccagucuagc gaggauaaag acauugaaaa gcggaauaaa      720

gcuguggcca cugagaucuu gaaggcgucc cuggggaaca aagcaaagcu aaacguaauc      780

acaaauguug agguggauaa ggaagccgcu aaagaguggu caauuaccuu cgacagugaa      840

agcauagaug acgaucucgc caagaucgag ggacaaauga cggacgaugg ccaugaaauu      900

aucgaggucc ugcgcucucu uuacuccggc auaacucugu cagcaauugu gccugagaac      960

cacaccuuga gccagaguau gguggcuaaa uaugaccucc acaaggacca ucugaaauua     1020

uuuaagaagc ugaucaaugg gaugacagau acuaaaaagg ccaaaaaccu cagagcggcc     1080

uacgacggau auaucgaugg ugucaagggc aaaguucuuc cacaggaaga cuucuacaaa     1140

caggugcaag uaaaucugga ugacucugca gaggcuaacg aaauucagac cuauaucgau     1200

caggacauuu uuaugcccaa gcagaggaca aaagccaaug gguccauacc ucaccaauug     1260

cagcagcaag agcuagauca gaucauugaa aaccagaagg cuuacuaucc auggcuggcc     1320

gagcucaauc ccaacccuga caaaaagcgu cagcaacugg caaaauacaa acuugaugag     1380

cuggugaccu uccggguccc auacuaugug ggaccgauga ucacggccaa ggaccagaaa     1440

aaucagagcg gcgcugaauu ugcguggaug auccgaaagg agcccgguaa cauuacuccu     1500

uggaauuucg aucagaaagu ugacagaaug gcaacagcca accaauuuau aaagaggaug     1560

accacuacag acaccuacuu gcucggggaa gaugugcugc cagcucaguc guuacuguau     1620

cagaaauucg agguccuaaa ugagcucaac aagauccgca uugaccauaa acccaucucu     1680

auugaacaga agcaacagau cuuuaacgau cuguucaagc aguuuaaaaa ugugacgaua     1740

aagcaccuuc aggacuacuu gguaucccaa ggacaguauu caaaacggcc ucugaucgag     1800

ggccucgccg augaaaagag auucaacagu agccugucca ccuacucuga ccuuugcggc     1860

auuuuugggg caaaacuggu ugaggaaaau gauaggcagg aggacuugga gaagaucauu     1920

gaauggagca caaucuucga ggauaagaaa auauaucgcg ccaagcucaa cgaccugacu     1980

ugguuaaccg augaccaaaa agaaaagcug gcuacaaaac gauaccaggg auggggucgg     2040

cuaucacgua agcuccuggu gggccuuaag aauagugagc acagaaacau uauggauauc     2100

uuguggauua cuaaugaaaa cuucaugcag auccaggccg agccagacuu ugcaaaacug     2160

gucaccgacg cgaauaaggg gaugcucgag aaaacagauu cccaagacgu gauaaacgau     2220

cuguacacca gcccccagaa uaagaaagcu aucaggcaga uuuuacuggu cgugcaugac     2280

auccagaacg ccaugcacgg acaagcaccg gccaagauuc auguugaguu cgcucgcggc     2340

gaagaacgga auccuagaag gucugugcag cgacagcgcc agguagaggc cgcauaugaa     2400

aaggucucaa acgagcuugu gagugcuaaa guucggcaag aguuuaagga agccaucaau     2460

aacaaaagag auuucaagga cagguuguuu cucuacuuca ugcagggugg gauagauauu     2520

uauacuggca aacagcugaa caucgaccaa cuaagcuccu accagaucga ucacauucug     2580

ccccaggcgu uugugaagga cgauucucuc acgaaucgug uccugacaaa cgagaaucag     2640

gugaaagccg acucgguacc aaucgacauu uucggaaaga agaugcuuag cguguggggc     2700

cgcaugaaag aucaaggguu gauauccaag ggaaaauauc ggaaccugac caugaauccu     2760

gaaaacaucu cagcucauac ugagaauggu uuuauuaaca gacaguuagu ugagaccagg     2820

caggucauca agcucgcagu gaauauucug gccgacgaau acggcgauag uacacagauc     2880

auaucuguga aagcugaccu uagccaccaa augcgagagg auuucgaacu guugaagaac     2940

cgggacguca augauuauca ccaugccuuu gacgcauacc ucgcggccuu caucgggaac     3000

uaccugcuaa agcgcuaucc caaacuggag uccuacuuug uuuauggaga uuucaagaaa     3060

uucacccaga aggaaacuaa aaugagaagg uuuaauuuca uuuacgaccu caagcacugu     3120

gaucaggugg ugaacaagga gacaggcgag auccugugga cgaaagacga agauauuaag     3180

uauauccggc auucauuugc uuacaaaaag auacuuguaa gccacgaggu ccgagaaaag     3240

agaggugccu uguauaauca gaccauuuac aaggcaaagg acgacaaagg gucuggccaa     3300

ggauccaaga aacugauccg caucaaggau gacaaagaga cuaagauuua cggcggguau     3360

aguggaaaga gcuuagcuua caugacaaua gugcagauca ccaaaaagaa caaaguuucu     3420

uauaggguga uugguauccc aacacucgcc cuggcacguc uuaacaagcu ggaaaaugau     3480

uccacugaga acaauggcga gcuauacaaa auuaucaagc cccaguucac ccacuauaaa     3540

gucgacaaga agaacgggga aauaaucgag acgaccgaug acuuuaaaau ugugguuuca     3600

aaggugcggu uccagcaacu caucgaugac gccggacagu uuuucaugcu ggcuagugau     3660

acauacaaaa auaacgcgca gcaauuggua auuagcaaua acgcccugaa ggcaaucaau     3720

aacacuaaua uaaccgacug cccuagagau gacuuagaaa ggcucgauaa ccugcgccuu     3780

gacucggcuu uugacgagau ugucaaaaag auggauaagu auuucuccgc cuacgacgcc     3840

aauaacuuuc gagaaaagau cagaaauucu aaccugaucu ucuaccaguu gccaguggag     3900

gaucaguggg agaauaacaa gauuacagaa cucggcaaac ggacugugcu gaccaggauc     3960

cuacaggguc ugcaugcuaa cgcaacgaca accgacauga gcauauucaa gauuaaaacu     4020

ccguuugggc aacuucgcca gcguucaggc aucagucucu ccgagaaugc ccagcugauu     4080

uaucagucuc ccacaggauu guucgaaaga cggguccaac ugaacaagau caaguga        4137


<210>  3197
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3197
augaccaacg agaaguacag caucggccug gacauuggga cauccucuau aggauucgcc       60

guggucaaug auaacaauag aguuaucagg gugaaaggua agaacgcuau uggcguacgg      120

cucuuugacg aagggaaagc agccgcggau cgccgaucau uccguacuac gagaaggcgg      180

cuuagucgca gaagguggcg gcugaaguug uuacgcgaga ucuuugacgc uuauauuacc      240

cccguggaug aagccuucuu uauccgacug aaagagagca aucuaucccc uaaggacucu      300

aagaaacagu acucgggaga uauacucuuc aacgacagau cagauaagga cuuuuaugaa      360

aaauacccaa caaucuauca ccugaggaau gcacuuauga cugagcaucg gaaguucgau      420

guccgugaga uuuaccuggc cauccaccau auuaugaaau uucgcggcca cuucuugaac      480

gcuaccccgg caaauaacuu uaaggugggu agacucaauc uggaagagaa guucgaagaa      540

cugaacgaca ucuaucaaag gguuuuuccc gaugagagca uagaguuccg gacagacaau      600

cucgagcaga uuaaagaagu guuacuggac aacaagcgaa gucgcgccga uagacagagg      660

acccuugucu ccgacaucua ccagucuagc gaggauaaag acauugaaaa gcggaauaaa      720

gcuguggcca cugagaucuu gaaggcgucc cuggggaaca aagcaaagcu aaacguaauc      780

acaaauguug agguggauaa ggaagccgcu aaagaguggu caauuaccuu cgacagugaa      840

agcauagaug acgaucucgc caagaucgag ggacaaauga cggacgaugg ccaugaaauu      900

aucgaggucc ugcgcucucu uuacuccggc auaacucugu cagcaauugu gccugagaac      960

cacaccuuga gccagaguau gguggcuaaa uaugaccucc acaaggacca ucugaaauua     1020

uuuaagaagc ugaucaaugg gaugacagau acuaaaaagg ccaaaaaccu cagagcggcc     1080

uacgacggau auaucgaugg ugucaagggc aaaguucuuc cacaggaaga cuucuacaaa     1140

caggugcaag uaaaucugga ugacucugca gaggcuaacg aaauucagac cuauaucgau     1200

caggacauuu uuaugcccaa gcagaggaca aaagccaaug gguccauacc ucaccaauug     1260

cagcagcaag agcuagauca gaucauugaa aaccagaagg cuuacuaucc auggcuggcc     1320

gagcucaauc ccaacccuga caaaaagcgu cagcaacugg caaaauacaa acuugaugag     1380

cuggugaccu uccggguccc auacuaugug ggaccgauga ucacggccaa ggaccagaaa     1440

aaucagagcg gcgcugaauu ugcguggaug auccgaaagg agcccgguaa cauuacuccu     1500

uggaauuucg aucagaaagu ugacagaaug gcaacagcca accaauuuau aaagaggaug     1560

accacuacag acaccuacuu gcucggggaa gaugugcugc cagcucaguc guuacuguau     1620

cagaaauucg agguccuaaa ugagcucaac aagauccgca uugaccauaa acccaucucu     1680

auugaacaga agcaacagau cuuuaacgau cuguucaagc aguuuaaaaa ugugacgaua     1740

aagcaccuuc aggacuacuu gguaucccaa ggacaguauu caaaacggcc ucugaucgag     1800

ggccucgccg augaaaagag auucaacagu agccugucca ccuacucuga ccuuugcggc     1860

auuuuugggg caaaacuggu ugaggaaaau gauaggcagg aggacuugga gaagaucauu     1920

gaauggagca caaucuucga ggauaagaaa auauaucgcg ccaagcucaa cgaccugacu     1980

ugguuaaccg augaccaaaa agaaaagcug gcuacaaaac gauaccaggg auggggucgg     2040

cuaucacgua agcuccuggu gggccuuaag aauagugagc acagaaacau uauggauauc     2100

uuguggauua cuaaugaaaa cuucaugcag auccaggccg agccagacuu ugcaaaacug     2160

gucaccgacg cgaauaaggg gaugcucgag aaaacagauu cccaagacgu gauaaacgau     2220

cuguacacca gcccccagaa uaagaaagcu aucaggcaga uuuuacuggu cgugcaugac     2280

auccagaacg ccaugcacgg acaagcaccg gccaagauuc auguugaguu cgcucgcggc     2340

gaagaacgga auccuagaag gucugugcag cgacagcgcc agguagaggc cgcauaugaa     2400

aaggucucaa acgagcuugu gagugcuaaa guucggcaag aguuuaagga agccaucaau     2460

aacaaaagag auuucaagga cagguuguuu cucuacuuca ugcagggugg gauagauauu     2520

uauacuggca aacagcugaa caucgaccaa cuaagcuccu accagaucga ucacauucug     2580

ccccaggcgu uugugaagga cgauucucuc acgaaucgug uccugacaaa cgagaaucag     2640

gugaaagccg acucgguacc aaucgacauu uucggaaaga agaugcuuag cguguggggc     2700

cgcaugaaag aucaaggguu gauauccaag ggaaaauauc ggaaccugac caugaauccu     2760

gaaaacaucu cagcucauac ugagaauggu uuuauuaaca gacaguuagu ugagaccagg     2820

caggucauca agcucgcagu gaauauucug gccgacgaau acggcgauag uacacagauc     2880

auaucuguga aagcugaccu uagccaccaa augcgagagg auuucgaacu guugaagaac     2940

cgggacguca augauuauca ccaugccuuu gacgcauacc ucgcggccuu caucgggaac     3000

uaccugcuaa agcgcuaucc caaacuggag uccuacuuug uuuauggaga uuucaagaaa     3060

uucacccaga aggaaacuaa aaugagaagg uuuaauuuca uuuacgaccu caagcacugu     3120

gaucaggugg ugaacaagga gacaggcgag auccugugga cgaaagacga agauauuaag     3180

uauauccggc aucuuuuugc uuacaaaaag auauugguau cacacgaggu ccgagaaaag     3240

agaggugccc uguauaauca gaccauuuac aaggcaaagg acgacaaagg gagcggccaa     3300

ggaucuaaga aauuaauccg caucaaggau gacaaagaga cuaagauuua cggcggguau     3360

uccggaaaga gucucgcuua caugacaaua gugcagauca ccaaaaagaa caaaguuagc     3420

uauaggguga uugguauccc aacacuggcc cuugcacguc ugaacaagcu agaaaaugau     3480

ucuacugaga acaauggcga gcucuacaaa auuaucaagc cccaguucac ccacuauaaa     3540

gucgacaaga agaacgggga aauaaucgag acgaccgaug acuuuaaaau ugugguuucc     3600

aaggugcggu uccagcaacu gaucgaugac gccggacagu uuuucauguu ggcuucagau     3660

acauacaaaa auaacgcgca gcaacuggua auuaguaaua acgccuuaaa ggcaaucaau     3720

aacacuaaua uaaccgacug cccuagagau gaccucgaaa ggcuggauaa ccuucgccug     3780

gacagcgcuu uugacgagau ugucaaaaag auggauaagu auuucucggc cuacgacgcc     3840

aauaacuuuc gagaaaagau cagaaauucc aacuugaucu ucuaccagcu cccaguggag     3900

gaucaguggg agaauaacaa gauuacagaa cugggcaaac ggacugugcu aaccaggauc     3960

cugcaggguc uucaugcuaa cgcaacgaca accgacaugu cuauauucaa gauuaaaacu     4020

ccguuugggc aacuccgcca gcguagcggc aucucacuga gugagaaugc ccaguugauu     4080

uaucaguccc ccacaggacu guucgaaaga cggguccaau uaaacaagau caaguga        4137


<210>  3198
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3198
augaagcaga uccugggccu cgaccuuggg accacaagca uuggaugggc ccuggugaga       60

gaggcugaaa acaaagauga gcaauccucu auagucagga ucgguguucg ggugaauccc      120

uugacuucag acgaacagaa gaacuucgag cagggcaaaa guauuacgac caaugcagau      180

cgcacauuaa agcgaagcau gcguagaaac cugcagaggu acaaacuacg gcgcgaccac      240

cucaaggauu gccugcaaaa gaaugggauc auuacugaca aagccguacu uuccgaaaac      300

ggaaauuaua ccacauuuca gaccuacuau cugagagcga aggcugugaa cgagaaaauc      360

ucuuuggagg aauuugccag gguccuccug augcugaaua agaaacgggg cuacaagucg      420

ucacgcaaga ugaaaagcgc agaugagggu cagcucauag acgggaugag uguggccaag      480

cgauuauaua acgauaauau cacuccugga caguacguuc ugucccuuuu ggaagagggc      540

agaaaauaua uuccagacuu uuacaggucu gaucugcuag acgagcucga uaagaucugg      600

acauuccaac aguguuuuua cccggaaauu cugacccccg agugccggaa acuuaucgcu      660

ggcaagggga aacaggcaac ggccaagaua uuccugggaa aguauaaaau uuacacugcu      720

gacaacaaga gcaaagacaa gcguuugcag gccuaucaau ggcgcgugga agcgcucucc      780

ggucagcugc cuaucgaggu cguggcauuu guaguuugug aaauuaaugg cgccauccag      840

gcuucaagug gguacuuagg agccaucagc gauagaucua aagagcugua uuucgagggc      900

cucaccgugg gucaauaccu ugucaagcag cuggaaaaga acccagacac auccuugaaa      960

aauaaggugu uuuauaggca ggauuaccua aacgaguucg agaaaauuug ggagaagcag     1020

gcagaauacc auacugagcu gacuccccaa cucaaaaagg aggugcggga cauaaucauu     1080

uucuaucagc gaacacugaa gucacagaaa gggcuugucg gauucugcga auuugagagc     1140

cgccagaucg aaguuaugau agauggcaag cugaaaaaga aaaccguggg cuguagagua     1200

ugcccuaaga guucuccauu guuccaagag uacaagauuu ggcacaaccu caauaaccug     1260

aaaaucgugg aaaaggaguu agggaaaucc agguuccuga aucaggacga gaaggaaagc     1320

cuauuucagg agcucaacau caaagaaaag cugucgaaaa cggauauucu uaaguugcug     1380

ugucucaagg cuaaagaguu cgaccugaau uuugagaagg ucgaaggaaa ccggacucag     1440

gccgagcuuu uccgcgcgua ucaagccauc auugaaaugu cugguaaugg cgauuacgac     1500

uuuuccaaaa ugacaucaag ugagauacag gauaccguga cugggguuuu cagaggacug     1560

ggcuauaaca cagacaucuu gagcuuugac gcagaaaagg aggagaaaac cuucgauaag     1620

cagcccaugu accuccugug gcauuuacug uauuccuacg aaggugacuc uagcgauacg     1680

gggaauaagg cucuaauuac caaaaucaag aaacucugcg gcuuugaguc agaagaguac     1740

gccaggaucc ugacagacgu gacuuuccag agugaauaug gaaaccuuuc caccaaggcu     1800

auucgucgga uauugccuuu uaugaaaaag ggcagcgagu acucugaggc cugucgacac     1860

gcaggguaua gacauucaga aaggagucug acaaaggagg aacucgauaa aaaggucuac     1920

aaagaccacc uggaguuacu gccacgcaau agccuucgga acccgguggu agaaaagauc     1980

uugaaucaaa ugauucacgu ugugaacacu gucguggaua ccuauggaaa acccgacgag     2040

aucagaauug agcucgccag ggaacugaag aaguccgcua aagagcgcga ugaaaugaca     2100

gcgucuauca auaagaccgc agccgagcau aaaaaguacu gcgagguccu acagaaagag     2160

uucgguuuua cucacguguc gcgaaacgac auaauccggu auaagcugua ccucgaacug     2220

gaaaacaaug gcuacaaaac gcuuuauagc gauacauaca uuccucguga gaaguuguuc     2280

uccaaggagu ucgacaucga acauauuauc ccacagucaa aacuguuuga ugacaguuuc     2340

ucuaacaaga ccuuagagug uagaagcguu aauauagaaa aauccaacga cacugcuuau     2400

gauuacgugc ucaauaagua uggggagcaa ggcauucugg acuacaaaaa caggaucgaa     2460

aaucuuuaua aggauucaaa gauuagccgc accaaauacc agaagcugau gaugaaaaac     2520

ucugacaucu ccggaaauuu uauagagcgg gaauugagag auagucagua caucgccaag     2580

aaagcaaggg agauucucga ggaacuggua aaguucgucg ugcccacaac cgguagcguu     2640

acugcccgac uacgcgagga cuggcagcug guggauguca ugcaagaacu caacuggaag     2700

aaauaugaca agcuggggcu uacagaugug cgguacgaca aagauggcag agagaucagg     2760

uauauuaagg acuggucuaa acguaaugau caccgccacc augcuaugga cgccuugacg     2820

aucgcauuua ccaagcgguc cauaauucag uaccugaaca auuuaaacgc gaagucagac     2880

aaaaguggaa gcaucuaugc uaucgaacag aaggaacucu ucagagauaa aaggggcaag     2940

cugaaauuua uuccuccaau gcccugcaau aucuuccgag ccgaggcaaa gcggcaccuu     3000

gaaaacguac uggugucggu uaaggccaaa aacaaggucg ugacuccgaa uauuaaccgc     3060

uccaaaggga agaauggaau aaacaaaaag auccagcuaa caccuagagg ucaacuccau     3120

aaugagacca uuuacggcuc uaugaaaagg uaugaaacaa agugggagaa gaucgggagc     3180

ggauuugacg agcggcagau ugcuaaagug gccaacaaga aauuccgaga agcacuguug     3240

ucaagacugg cugaguuugg caaugauccc cagaaggccu ucacugguaa aaacaguuua     3300

gaaaagaauc cacucuacgu cgacgagcug cacacccaga agguuccuga aaaagugaag     3360

aucgugacgc uugagaaccu guacaccaua cgcaaagaga ucacacccga uuugaagauu     3420

gacaaaguag ucgaugcggg ggugaggcgu guucuccaag aacggcugga cgaguuugau     3480

gacgauucca agaaggccuu cucuaaucua gacgaaaacc caaucuggcu gaauggcaaa     3540

aagaugaaaa gagugacuau uaugggaguc agcaacgcug ugccccuuca ugacaagaaa     3600

gauuauaagg gcaaucucgu uuucgacucc aaggggagga aacagccugu ggauuuugua     3660

aacaccggau caaaccacca cgucgcaauc uaccgcgacg agagcggcga acugcaggau     3720

auagugguga guuucuauga ggccacaacu cgaucuaccu uggguauucc agucaucgac     3780

aaggaguaca aaaaggcuga aggguggaau uuucuguucu ccaugaaaca aaacgaguau     3840

uuuguuuucc cgaaugaaga gacgggauuu aaccccaagg aaaucgauuu aaugaauccu     3900

gacaacuaca caaccauuag cccaaaucuc uauagagugc agaaauucuc gaagugcaug     3960

uacggcaacu caucuguacg ggaguacgug uuuaggcauc accuggagac cagugucaau     4020

gauuccaagg gucuuaaaaa cauagccuau aagaauguga aaagccuggu uuacuuggaa     4080

ggcaucguga agguccgcau uaaccauauc gggcagauug ugucuguugg agaguauuga     4140


<210>  3199
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3199
augagaaucg acaacggccu ggaggaacuc aaggugaaua acaggauuua caauauaggg       60

cuugauaucg gaaccagcuc cgucgguuuc gccguuacag acgcucagga uaaacuggug      120

cggguaaagg gcaaaacugc aauuggggug cgcuuguuug aggaaggaca cacggccgcg      180

gagcgacgug gcuucagaac cacaaggcgg cgcuuaucua gaaggaagug gcggaucaac      240

cugcuaaauc aaauuuuuga acccuauauc ucagagauag acccuacuuu cuuugcucgc      300

cucaaaaaca guagccuggc cgguaaggau aagaaauucg aggggucccu ucuguuucca      360

gaccgaaccg auuaccaguu cuauaaggac aauccgacaa uguaccauuu gagaaaacac      420

cucauggaaa aggauaggca guuugaccug cgggagaucu aucuggcaau gcaucacauu      480

aucaaauacc guggaaacuu ccucaauacc acucccauga accauuuuaa gaaugaagag      540

auugauuucu cugccuuauu uucgcgccug aacgagcuuu aucaggaaau cgacgaugag      600

aauauauucg aauugaacau ugagaauguc gacgaaauca agaaaauucu gcuaaaccac      660

gaccucuuca agauggagaa acaaaaacag guggcuaaac ugcuuaacgu ugauacagac      720

gauaagcagc ugaaaaagga gaauaccgca uuggccucaa acuuuagcaa ggcuauccuc      780

ggcuacaaau ucaaucuggc cuuagugacg aagaucccug accaggauaa aaaggaaugg      840

acuauuaguc ugggcgacga ggauuuugac gauagacucg acaacguccc aguggcgcuu      900

accgaauccc aacuggaggu aguugaagug uugcacucua uauucaauag caucucccua      960

aacgagauug ucgagaaugg ggaaacacug ucaagugcaa uggugaggaa auacaacgau     1020

cauaagcacg accucaagcu gcuuaaaaau gugaugacua cccuggagga ccggaagaaa     1080

gccaagaaaa uccagggaau auauaacguc uuuguuggua aggauacaga cuugauuagc     1140

gauaaggaca aaaauaagua caaagauaag gacgaguucu auaaagaaau ccagaagaac     1200

cucgauaauu cuuccgacgc ugaagagauc auuaagcgaa ucaaacagga ucaauuucug     1260

cccaagcagc gcaccucaaa gaacggcgug auuccucauc aguuacacca aagagagcug     1320

gaccuaauaa ucgaaaauca ggccaaguac uauccauucc ucaaagaggc aaaccccaac     1380

aagaaaaggc ugaagcaggc uaaguacaaa cuugaugaau ugauugccuu uaagaucccu     1440

uauuacaucg ggccaacgag cggagcggag aauagugacu ucucuuggcu gaaacggaag     1500

aaagauggcc agauuacucc guggaacuuu gacgagaagg uagaccgcau agaauccgcc     1560

aauagauuca ucaggcguau gacaaccaag gauacuuacc uccuggcaga ggacgugcuu     1620

ccccuggaau cauugauuua ugagaaauac ucgguccuca acgaacugaa uaacgugcgg     1680

aucaaucgac aaaaguuauc uguuaacgau aaacaggaca uuuauaauga gcuguuuaag     1740

aacuccaaaa cagugacccu aaagcagcuc cagaaguacu ucgaggucac gaaaaagcug     1800

aaaaccgugg uaguugaagg ucuugcugau ccuaagaaau ucuugucaaa ucuggggaca     1860

uaucacgacu uuaagcucau cuucggagau auaguggacg auccagagua ccaaacugac     1920

cuggaaaaca ucauugagug gaguaccauc uuugaagaua gaggcauuuu caauagcaag     1980

uuaaacgaga ucacauggcu gacugacgag cagaggaaaa agguccuuuc caaacgcuau     2040

cagggcuggg ggcgguuuuc uaagaaauug cucgugggac ugaagaauag ccaggguaac     2100

ucaauacuag augaacugug gaacaccaau cucaacuuca ugcaacugca gagucaggac     2160

gacuuugccg agcuuauuca ugcugaaaau gccaaacagu ucaaggcaaa cgaucccgac     2220

aauccuugga acggcaucga ugccauuuug aaugacgcuu acacaucccc acaaaacaag     2280

aaagcgauca gacaggucgu gaagguugug caggauauag agaaagcaau ggggaauaag     2340

ccccagaaaa ucgccauuga auuuaccagg agcgcugaca agaacccgcg ccgaacugau     2400

ucucggauca agcgucuguc agacauuuac aaagaggccg cagagaaggu agucggagaa     2460

aguuuaaaac ucgagcugaa ugaauaucuu acgagcaaga aaaagaugac agauaagaug     2520

uaccuguauu ucacccaauu gggcacugac aucuacaccg guaaaaagau agauauugac     2580

cagcucaaca auuaugacau cgaucacgug cugccucagu ccuuucuaaa agacgauucu     2640

cuggacaaca gaguucucac aaauaggucg gauaacagcg agaaguccga caccgugccc     2700

cugcauaaau ucggggccaa gaugggcaac uuuuggcgcc aacuugcuga ugagggauug     2760

aucacuaagc ggaaauacaa gaaucugaca acggacccag ccaccauuga uaaauauacu     2820

auccagggcu ucauuagaag gcaguuaguc gaaacaucac aggugauaaa gcucaccgca     2880

aacauccuug ggggacugua cccugaggaa acagagauuc uggaaauccc cgcgaaacua     2940

aaucaccaau uccgagacau guuugaucuc guaaaggugc gcgagguuaa cgacuaccau     3000

cacgcuuucg augccuauau uacugucuuu guggguaauu uccuguaccg gagauaucca     3060

aaguugagga guuacuuugu guauggcgac uucaaaaagc ugaacaaaac cgacgaugac     3120

auccguaagu uuaauuucuu aaaccucuuu aauaaagaua aggucgagga caacgaaacg     3180

gaugagauac uguggucuaa agcagaaagc auccgcgaca uuaagaauau cuacaacuau     3240

aaguucaugc uuguuucccg ggagguguac accagacacg gggccauguu uaaucagaca     3300

auuuaccccg cuagcaaaaa cgauagcaag aaacugaucc cuauaaagaa aggaaaggac     3360

acugccauuu auggcgguua cucugggaau accgaugcau auauggcuau cguggccgua     3420

gaagacaaga aaggcgagaa guucaaaguc gugggaguuc caacaagggc ggugucccga     3480

uuggagcagc ucgccgcuga ugaccaugau caguaccuga cugcacuaca cgacguccug     3540

aagccgaacu ucaccaaaau caagacgaau cggaagacaa aagccaagga agaggugacc     3600

acucccuuuc gcauucuugu uggcaaagug ggguauagac aacucauaca ggacggacag     3660

guaaagauga ugcuggguag uagcgcuuac aaauauaacg ccaagcaauu gguccugucu     3720

gaaaaguccu uaaaagugau caagaacaau aaaaaguaca acccugauga cgagaaucag     3780

gaucucauug acguguacga ugaaauccug ggcgucguua acagggcauu caaucuuuau     3840

gacacaaacg gguuucggaa aaagcugaau gauaaccgag ugaaauucgu agaguugcca     3900

auuaauaaca ucuuugacgg aaccaagcuc gugcagaugg gcaagagagc gguccugacu     3960

cagauacuaa auggucugca ugccaacgcu gcccuugggg aucucaaagc acugggcauc     4020

ucaacccccu ucggaaagau gcaaaguaaa agcggcauuc cuuugucgau ggacgcuucc     4080

uuaguguacc agucuccaac agggcuguuu gagcgcaagc ucaaucugag cgaucuuuga     4140


<210>  3200
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3200
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucauaacug augacuauaa gguucccgcu aaaaagauga aagugcucgg uaacacggau      120

aagaaguaca ucaaaaagaa ucuucugggc gcauuguuau ucgacucugg ggagaccgcc      180

gaagcgacaa gacugaaaag gacugcucgg cgccgauaua cccguagaaa gaacaggcua      240

cgguaccucc aggagauuuu ugccgaugaa auggcaaaag uagacgaguc auucuuucaa      300

cgccuggaug aaaguuuccu uagaugggac gaugacaaua agaagcuggg aagguauccu      360

aucuuuggca acaaagccga uguggucaag uaccaccagg aguucccaac aauuuaucau      420

uugcggaaac accucgcuga cagcuccgag aaggcagauc ugcgccuggu guaccucgcc      480

uuagcucaua ugaucaaauu ucgaggucac uuccugauag aaggggagcu uaaugccgaa      540

aacaccgacg uucagaagau cuuuucugac uucgugagag ucuacgauag gacuuuugac      600

gauucgcauu ugucagagau uacaguggac gcggcaagca uccugaccga gaaaauuagu      660

aagucccggc gucuagaaaa ucucaucaag uauuacccga cggagaaaaa gaacacucug      720

uucggaaauc uuauagcccu ggcuuugggc cuccagccca acuuuaaaau gaauuucaag      780

cugucugaag augccaaauu acaauucagc aaggacuccu auaacgagga ucuggaggaa      840

cuccuuggca agauugggga cgauuacgca gaccuguuua ccucagcuaa aaauuuguau      900

gaugccaucc uacugagugg aauuuuaaca guagacgaca acagcacuaa ggcgccucug      960

ucugccucca ugaucaaacg cuacgcagag caccacgaag aucuugagaa gcugaaagag     1020

uucaucaagg cuaacaaguc agaauuguau caugacauuu uuaaagauga aaccaagaau     1080

gguuacgccg gcuacauaga gaacgggguu aaacaggacg aguucuauaa guaccucaaa     1140

aauacacuga gcaagaucgc uggaagugau uauuuucucg acaagauuga aagagaggau     1200

uuccugagga aacagcggac cuuugacaac ggcucuaucc cacaccagau acaucuacaa     1260

gaaaugcacg ccauucuccg acgccagggu gauuacuauc ccuuccugaa ggagaaucag     1320

gacagaaucg agaaaauccu uacguuuagg auuccuuacu auguggggcc auuggcacgg     1380

aaggauuccc gcuucagcug ggccgaauac cacucggacg agaaaaucac ucccuggaac     1440

uuugauaagg ucauugacaa agaaaagucu gcugagaagu ucauaacaag aaugacccug     1500

aaugaccucu accugccuga agagaaagug cuuccaaagc auucccacgu guaugagacu     1560

uacgcggucu auaacgaacu gacaaaaauc aaguacguua augagcaggg aaaagauuca     1620

uuuuucgaca guaacaugaa gcaagaaauu uucgaucaug uguuuaagga gaauaggaaa     1680

guaaccaagg aaaaauugcu caacuaucug aauaaggagu ucccggagua ccguaucaaa     1740

gacuuaaucg gccuggauaa ggaaaacaag agcuuuaaug caucccuagg cacguaucac     1800

gaccucaaaa agauucugga uaaagccuuc cuugacgaua aggugaacga ggaagucaua     1860

gaggacauca uuaaaaccuu gacacuguuu gaagauaagg acaugaucca ugagcggcuc     1920

cagaaguacu cugauauuuu cacugcugac cagcugaaaa aguuagagcg aagacacuac     1980

accggguggg gaaggcugag cuauaaacuu aucaacggua uacgcaauaa ggaaaacaau     2040

aaaacaaucu uggacuaccu cauugaugac ggcucagcca accggaauuu uaugcaacug     2100

aucaacgaug acacucuacc cuucaagcag auuauccaga aaagucaggu gguuggggau     2160

guggacgaua uagaggcagu cgugcacgac cugccuggau ccccagccau uaagaagggc     2220

auccuccaaa gcguaaaaau uguugaugaa cuggugaagg ucauggguga caaucccgau     2280

aacaucguga uagagauggc uagagaaaau cagaccacaa acagggggcg cucucaguca     2340

cagcaacgac uuaaaaaguu gcagaauagu cugaaagagu uaggcagcaa cauccucaau     2400

gaggaaaagc cuuccuauau ugaggacaag gucgaaaacu cucaucugca gaaugaccag     2460

cuuuuucugu acuauaucca aaacggaaaa gauauguaca ccggcgacga guuggauauu     2520

gaccaccucu cggauuauga caucgaucau auaauuccac aggccuucau caaggacgau     2580

agcaucgaca accgggugcu gacuuccuca gcgaaaaauc gugggaagag ugaugacguu     2640

cccucucuag auauugugag agcaaggaaa gcugaguggg uacgccugua caagagcgga     2700

cucaucucca agcggaaauu ugacaaccug acgaaggccg aaagaggugg ccuuacagag     2760

gcagacaaag ccggguucau uaagaggcag uuggucgaaa cccgacagau aacuaaacac     2820

guggcucaaa uccuggaugc ccgcuuuaau accgagucag acgaaaacga uaagguuauu     2880

cgggacguga aggucaucac auuaaaaagc aaucucgugu cucaguucag aaaggauuuc     2940

gaguuuuaca aaguaaggga gauuaacgac uaucaccaug cacacgaugc uuaccugaau     3000

gccgugguug gaaccgcgcu ucugaagaaa uauccgaagc uagccuccga guucgucuac     3060

ggcgaauaua agaaauacga cgugcauaag cucaucgcua aaaguagcga ugaccacucu     3120

gaaaugggua aggcaacugc caaauacuuu uucuauucca accugaugaa cuuuuucaag     3180

cgugugauac gcuacucaaa ugggaaaguc aucguucggc cuguggugga guauaguaag     3240

gauacagaag acauugcuug ggauaagaaa agcaauuuua gaacgaucug caagguauug     3300

ucguaccccc aggucaacau ugugaaaaag guugagaccc aaacuggcgg auucuccaaa     3360

gagucuaucc ugccaaaggg cgacagcgac aaguuaauac cuaggaaaac aaagaaagcc     3420

uauugggaua ccaagaaaua cgggggauuu gacucaccca caguggcaua uagugucuuc     3480

gugguugcgg auguggaaaa ggguaaggcc aaaaagcuca aaacuguaaa ggagcugguc     3540

ggcauuucca ucauggaacg aucuuucuuu gaggaaaauc caguggaguu ccuugagaac     3600

aaaggguacc auaauauccg ggaagacaag cugauuaagu ugcccaaaua uagccucuuu     3660

gaguucgaag gaggcaagcg cagacugcua gcuuccgccu cagagcugca gaaagguaac     3720

gagaugguga uaccugggca ccuugucaag cuccuguacc acgcacagag gaucaacagc     3780

uuuaauagua ccaaauacuu ggauuauguu ucugcucaua agaaagaguu cgaaaaggug     3840

cuguccugug uagaagacuu ugccaacuua uacguggaug ucgagaagaa ucucagcaaa     3900

auucgggcag uggccgacuc gauggauaac uucucaaucg aagagauuuc uaauaguuuu     3960

aucaaccugc uuacgcugac cgcuuugggc gcgccagccg acuucaauuu ucucggagag     4020

aagauaccgc gaaaaagaua uacauccacu aaggaaugcc ugaacgcaac ccuaauccac     4080

cagagcauua caggccugua cgagacucgc aucgaucuuu cuaaaauugg ggaagaguga     4140


<210>  3201
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3201
augggcaagu acaaccuggg gcucgacauc ggaaccaaua gcguggguug ggccgucguu       60

gaugagaaca aucagauugu gaaaaagggc gggaaagcuc uuuggggagu aagaauguuc      120

gacgaagcac aauccgccaa ggagacaagg auauaucgga acucucgccg acguaucgug      180

agaaggaauc agcggcuguu guuacugcag aaagaauuuu acgaugagau uaucaagguc      240

gaccagacuu ucuuucaacg ccuaaaggau ucauucuuua aaaaggaaga caaacucaac      300

aagaauaacu auacguucuu ugaugacgau auuaccgaca gagaguacuu caaaaaguuu      360

cccacaaucu aucaccugag gagucaucuu cuguacaaug augagaagau agacauccgg      420

auguuguauc ucgcgcugca ccauauugug aaauaccgcg gccacuuccu ggauagcggu      480

gaaguuaacg aguccaauuu uucuaagauc aaagacgugc ucgacacuuu caacaauaag      540

augucggaaa ugucauuaga guuugaggau aacgaagacu acuucugcaa aauugaguuc      600

gaugaaaauu uugagaacaa gcugaaagac aucaugcuua gccaguugag uaagaaggaa      660

aaacgacagg agcugauaga ucuauucaag gucgacaaaa agucccucgu gaacgagcug      720

cuuauugaac uguugguaaa uaaaaagguu aagcucaacu cucugagcau caccaauggg      780

aaaaaguaug agguguguga aauugauuuu gagaaagaag aguuauucga gaagcuggaa      840

gacgcuaaaa agaucaucac agagcucuuu gauauucuug acgaaauagu caacaucaag      900

aaaauugugg auuacuauua cgugcugaag aucauaggag acaaugagua uuuguccgaa      960

gccaugguca aaaaguacga caaacaugau aaggaccuaa gagagcugaa gucauucauu     1020

aaaaccuauu gcccuaacaa guacuacgag uguuuuagga aaacaaguaa uccaaaccuc     1080

ugcaauuauc cgaaguacgu uggcaucaac agcgugaaua aaaagaucga acgguucgca     1140

cacuguaaaa aggaggaauu uuauaaguac cugaaaucuc uuuucaacac uauuaaggau     1200

gagaaugcca aacugaagau cgacuuguuu cagaaagaua uggagaacga cgauuuccuc     1260

aaucgucaaa acuccaacca caauucaagc auucccaugc agcugaacuu uugcgaauua     1320

aagaagauac uggagaauca gaguaaauau uacccuuucc uaaugcucaa gaacucugac     1380

ggcuauucca ccagcgaucg caucauugcu aucuucaaau acaagauccc auacuaugua     1440

gggccccuga auaaaaacuc gaaguacucu uggcuuguga agaaauccaa ugaaaacauu     1500

acgccuugga auuaugagaa ggucauagac uuggaugcau cagccaaaga auuuauccag     1560

agaaugcaaa acaaguguac uuaccugaaa ggagagaaug acuauugccu cccaaagaag     1620

aguauucugu ucagcgagua caacugucuu caguaucuga auagguugau cauuaacggu     1680

gcucucaucg aaaauaaacu gaagaacgag auauacgaaa acaucuuuuu augcuacaaa     1740

aagcccacca aaaaguccau uguggaguau cugaaaacaa auuacaacua ugaugaaacu     1800

acccuaaagu cuaauauccu cgagguuaac ugugacauga gcucauacau ucaguucaag     1860

gagaucuaug gcgaagacuu ugagcaaaau cugaacauag uggaagauau uaucaaagac     1920

auuacaaucu ucgaggauaa gaaaauacuu gaaaagcggu ugcgagagau cuaccaucug     1980

agugaggaca aaguccagaa gauuaagggg cucaauuaua aaggauacgc cacccugucc     2040

aagcacuuuu uaaacaauuu cuacagcgug aacgaagaga cgggcgaaau cuaugguccu     2100

auuauccaga uaaugcgcga uacuaauuac aaccugcagc aaguaauuua uaaugacaga     2160

uacaacuuua ugaaagcgau cgauaaguau aauaaaaaga accuugacac agagaagaaa     2220

uugaacguuu cugauuucau cgacgaguac auuuaugugu caccaaucau gaagaggagu     2280

cucauucaga gcuacaauau aauccaggaa cuggagaaaa uucuagggga auccaucucu     2340

aaguacuaua uugagugcgc acggguccgc ucgaacagca aagaugugau caauuccaga     2400

uacaggcagc ugucagaacu cuauaaggac ugcgagcauc aagucaguga uauaucuaag     2460

aacuacauca auauuaacaa acugaagaaa cagcuugagg acaauaagaa cgaauugcgu     2520

gccgacaaac uguauuuaua cuuuacccag uucggaaagu guauguauac ucucaaggau     2580

aucgaccugg augacguguu ucuuggcaaa uacgauauug accacaucua cccgcagagc     2640

cugauaaagg augacuccuu gaauaaccgg guucucgcuu caaaagagaa gaauggcaaa     2700

aaggauaacc aauucauuuu caauaucccc gaauuucugc cuaaaaacgc cgagcuauuc     2760

uauaagaagc ugcucgaaug caaacugauc acaaaggaga aauaccgaag acuuaccaug     2820

aagaaaauug acgaugacga guuggacaua uuugugaaua ggcagcuggu agcaacgaac     2880

cagagcguca agggguuagu ggauguucuc aagaucuaua aaggagugaa gccaaccaau     2940

auugucuacu cuaaagcugu gaacaucucc gacuuccgca acaauuauaa ccuguacaag     3000

agucggacag ccaauaacua ucaccaugcg cacgaugccu accuuaaugu agugauuggu     3060

aaagcacugg acguuuacua ucaguguaac uacuuucaaa auuauaagga ugucaucaag     3120

cuaaaagaca gaaacauaac uaucaauccc gaaauuaucc ucagcaagaa cagguacgug     3180

aaucgcaacg gcgagaaaaa ggugauuugg gaucuggaca auaccuugaa acugaucaag     3240

aacaauuuau auaaccgauu cgauauaaca gaaacuauua ugacccggaa gucuaacaaa     3300

auguuuucca agucaaguau cagcccugcu aaaaauucga agaaccucau cccaauuaaa     3360

acauccgacc cccguugcaa uguccugaag uacgggggaa ucaaaucuaa cagcuauaau     3420

uucuacugua uaguugagcu uuacgauaag aagggcaaaa agucauauau ucuggaagcc     3480

aucccgaaaa cccauaguga caaguuggag gaguacauua aaaacuccau cgaugaaaag     3540

aaugugucua acuuuaagau aauuaauaaa aacaucaaga gcaauaucau uaucgaguau     3600

aaaaagcuca gauacgugau uacugguaaa uccgggaagu auuaccugau aaagaacuuc     3660

aaagacagga auuuuucaua uaacgcuauc aagacgauua aauacaucga aaaguacaau     3720

uaucagaaaa aguacgagau cccuuucgag gaaacaaagg agaaaauuau acuagccccc     3780

aagaaaaagg acaccaaaga aaucagccug acucuugaua acugccaggc acuccuggac     3840

gagauuauca guuuguucaa gaaagauauu uauaacuacg acaucauaua uaagaucacc     3900

ucuaagcugg aagagaauaa acaaaacuua acacucacca aucugauuaa gguacuuacu     3960

gagauccuga uuuugcucaa aacaaacgaa cgcggcacgg ccgaucugac ccuaaucgac     4020

auguccaagg auacuggaac acuguguaua agcaaaaccc uuaagccagg caugaaguuu     4080

auuucggagu caccuacagg guacuaucgg aaaaucauuu ucgaaguccc caauggaauc     4140

uga                                                                   4143


<210>  3202
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3202
augaagagaa uccugggccu cgaccuuggg accacaagca uuggaugggc ccuggugaac       60

gaggcugaaa auaacaauga ggcauccucu auagucaggu uggguguucg ggugaacccc      120

uuaacuguag augaaaaauc aaauuucgag aagggcaaag ccaucacgac caacgcggac      180

cgccagcugc gacacggggc ucguauuaau cuacaaagau acaagcucag gcggcagaac      240

cugcaugauu gccuucagaa acagggaugg cugggcacag aagccaugua ugaggagggu      300

aaggcaagua cuuuugaaac cuacaaguug cgcgccaaag cugcagagga agagaucagc      360

cuccacgagu ucgccagagu gcuguuuaug cugaauaaga aaagggggua uaaguccaac      420

cggaaagcua auaacaagga agacggacaa cucuucgaug gcaugacaau ugccaagaaa      480

uuauacgagg aacaucugac cccugcggag uauucucuuc aguugcugaa uaagggcaaa      540

aaguuuacuc agggguacua ucgcucggac cuaaacgcag aacucgagcg aaucugggau      600

gagcagaaaa aguacuaccc agaaauacug acagacgagu ucaaacaaca gcuugaagga      660

aagaccaaga cgaauacuuc aaaaaucuuu cuggccaagu augguauuua cagcgcugau      720

uugaaaggcc ucgacagaaa guuccagccg cugaaaugga gggucgaggc cuuacagcaa      780

cagguggaua aggaaguucu ggcauuugug aucagugacc ucaaggggca gauugcuaac      840

accuccggac uucugggcgc caucucugau cggagcaaag aguuguauuu caacaagcaa      900

acagucgguc aguaccuaug ggcgucccug gaggaaaauc cccacauauc aauuaaaaac      960

aagccuuuuu aucgucagga cuaccucgac gaguucgaga aaaucuggga gacucaggcc     1020

gcauuucaua agcaacugac cccagaacuu aagcaggaga uucgcgauau caucauuuuc     1080

uaucagagac cccugaaaag uaagaaaucu uugauaagcg ugugugagcu cgaacagagg     1140

aagguaaaag cuacaaucga cgggaaggag aaggaaauua ccaucggacc uaaaguugcc     1200

ccaaaguccu cacccguguu ccaagaguuu cggauauggc agaaucugaa caaugucuua     1260

cugauugaua acgacacgaa ugagaaacga ccucuagaug aaguggagcg caaccuccug     1320

uacaaggaac uuagcaucaa agcuaaguug aguaaaacug aggcccugaa gauccucaau     1380

aagaaaggca agcaguggga ccugaacuac agagaacuug agggcaauag gacacaggca     1440

auucuguucg auugcuauaa ccggaucauu accuugacug ggcacgagga augugacuuu     1500

aaaaagauaa aagccucuga gauccgccac uacgugucca caauuuucaa gaaucucgga     1560

uuuagcaccg aaauccugga uuucgaccca ucguuaaaga aacaugagcu ggaaaagcaa     1620

ccgauguauc agcuauggca ccuccuguac ucuuaugagu ccgauaacuc aagaacgggu     1680

aaugagaguc uuuugaggaa acuggaaacc acauuuggcu uccccgagga auacgcuacu     1740

guccucugcg acguuguguu ugaggaagac uaugggaacc ugagcguaaa ggcgaugcgu     1800

gagaucuuac cuuaccugca ggcaggaaac gauuacuccc aggccugugc uuaugccggc     1860

uacaaucauu cucggcacag ccuuaccaaa gaggaauugg accaaaaggu guauaaggag     1920

cgacucgaac ugcuaccaaa gaacucacug agaaaucccg ucguggagaa gauucucaac     1980

cagaugauaa auguuaucaa cgcaauuauc gaugaguacg guaaaccuga cgaaauuagg     2040

aucgagaugg cccgcgaacu gaagaguucc gcugccgauc ggaaaaagac aacucacgca     2100

auaagccagg ggaaugcgga gaaccaaaga aucagggaaa uucuugagaa ggaguucucu     2160

uugucauaua ucagucgcaa ugacauuauc aaauacaagc uguaugaaga guuagaacca     2220

aacuacuaca aaacccucua uagcgauaca uacauaacca aggacaaacu guuuuccaag     2280

gauuucgaca uugagcauau cauucccaaa gcucgacuuu uugaugacuc uuucucgaau     2340

aagacucugg aagcccggaa caucaauuug gaaaagagca acaaaacggc auucgauuuu     2400

auaaaggaga aauauggcga agacggagcc gaggcuuaca agaaaaagcu cgacaugcug     2460

cuagaaaaug augccaucuc ccguccgaag uauaacaacc ugcucagagc agaggcugac     2520

auuccuucag auuucaucaa uagggaccug cgcaacacac aguacauugc caaaaaggcg     2580

ugcgaaaucc uuggcgaguu ggugaaaacc gucacuccca ccacagggaa gauaaccaau     2640

cggcugagag aggauuggca guuaguggac guaaugaaag aacucaacuu ugagaaguau     2700

gaaaagcugg gacuuacuga gauuguugag gauagggacg gucgaaaaau caagcgcauc     2760

aaagauugga caaagcggaa ugaccacaga caucacgcca uggaugcucu ggcaauugcc     2820

uucacgaaac caaguuuuau ccaguaccua aacaaucuca acgcuagguc uaauaagggc     2880

gacagcauuu acgccauaga aaacaaggag cugcauuaug aagaggggaa auugcguuuc     2940

aaugcaccua uccccgugaa cgaauuucgc gcggaggcca agcggcaccu guccgcuauu     3000

uuagucucaa ucaaagccaa gaauaaagug augacccaaa acgucaauaa gauuaagacu     3060

aaacacggaa ucauaaagaa aauccagcuc acaccaagag gcccccugca uaacgagacc     3120

auuuacggua caaagaugag gccuaucauu aaaaugguga agguuggggc agcucuugau     3180

gaagccacua ucaacaaagu gagcucucca gcaauacgag aggcccuguu gaagcggcuc     3240

aaugaauauu ccggcaacgc uaagaaagcg uucaccggaa agaauacgcu ggagaaaaac     3300

ccgauuuacc uaaaugccgg ccgcaccaag acaguaccca gucuggucaa aacuguggaa     3360

ugggagagcu uucacccuac cagaaaacuu aucgacaagg accucaacgu ugauaaagug     3420

gucgacaagg ggaucaggga gauucugaaa gcacgguugg aagaguucaa uggagaugcu     3480

aagaaagccu uuucuaaccu ggaagagaau ccaauauauu uagacgaagc caagaagauc     3540

gcucucaaac gaguguccau ugagggugua cugucagcaa ucccccuuca uacacugaag     3600

aaccaggccg gcaaaccuau uacugggaag gauggaaaac caguguuggg caauuacguu     3660

cagaccagua acaaucacca caucgcguuc uauuacgacg aggaugguaa ccuccaagac     3720

aaugcuguca gcuuuuucga agccgcagag agaaagucgc aggggauacc cgugaucgau     3780

aaggacuaca accgcgauaa aggcuggagg uuccuguuua cgaugaagca gaaugaauau     3840

uucguguuuc cgaacgaggc cacaggauuc auuccuuccg aggucgaccu aaccgacgaa     3900

gcuaacuacg gcaucauuuc uccaaaucug uaucguguuc agaaagugag ccggaucgau     3960

aaggggacuu cagcaaguag agacuacugg uuuaggcauc accuugagac aauacucaac     4020

gaugacgcca aacugaagaa uuuggcguuc aaacgcauuc gaggauuacu ggaacucaag     4080

gauaucauca aggugagaau uaacuccacc ggcaaaaucg uagcugucgg ugaguaugac     4140

uga                                                                   4143


<210>  3203
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3203
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggaugggc ccuggugaac       60

gaggcugaaa cugaguccga aaagucuuca auaaucagau ugggugucag gguuaauccc      120

uuaacgauug augagaaagg caacuucgaa aaggggaaag caaucaccac aaauagugac      180

cggcagcaac gccacagcgc ccgaauuaac cugcagcguu acaagcuaag aaggcagaau      240

cucugcgauu gucugcagau cggaggccuu cuggguuccg agucuaugua ugaggaaggg      300

aaggagucga cuuuugaaac cuacaaauug cgggcgaagg cugccacaga gaaaguggca      360

cuccaugagu ucgcccgcau acuguuuaug cugaacaaga aaagaggaua uaagucaaau      420

aggaaggcua acagcaaaga agacggccaa gcauucgaug gcaugaccau cgccaagaaa      480

cucuacgagg aaaauuuaac uccugcugag uauagucugc agcuuuugaa caaggggaaa      540

aaguuuuccc caucuuacua ucggagcgac cuggaauccg agcuaaaucg cauuugggag      600

gaacagaaaa aguacuaccc ggagauccuc acagaugacu ucuaucagca acuggaagga      660

aagaccaaag uaaacacgac uaagauuuuu cuugccaaau acgguaucua uaccgcggau      720

cugaagggcu uggacaaaaa gaugcagccc cucaaguggc gaauagaggc acugaauaaa      780

cagguggaua aggaagucuu aacauucgug guuucagacc ugaaagggca gauugagaag      840

acaaguggac uccuuggcga gaucagcgau agaucuaaag aacuguacuu uaacaagcaa      900

accguggguc aguaugucug ggccuccuug aaggagaauc cucacguguc aauuaaaaac      960

aagccauucu acaggcagga cuaucuagac gaauuugaga aaaucuggga aacacaagcu     1020

gccuuccaua aggagcugac ccccgagcuc aaaaaggaaa uccgggauau uauaaucuuu     1080

uaccagcguc cucuaaagag caaaaagggg cuuauuagua ucugcgagcu ggaacagaaa     1140

aaggagaaag uaauaauuga gggaaaggaa aaagagaucu ugaucggcuc uaagguugca     1200

ccaaagucca gccccaugua ccaggaguuc cgcauuuggc aaaaccucaa uaacgugcug     1260

uuaaucgaca cggaaauuaa ugaaucgaga ccucuggaug aggaggaaaa agcucuacuc     1320

uauaacgagc ugucugucag ggccaaacuu uccgaaacug aggcguugaa aacaauaaau     1380

aagaaaggca agcacuggga ccugaacuuc aaggaacucg aggggaaucg gacccaggcc     1440

gugcuguuug aguguuacaa ccgaaucauu acucagacag gacaugaaga gugcaauuuc     1500

aaaaagaucu cagcagaaga uauccgccac uauguggcua ccauuuuuaa aaaccuuggu     1560

uucaauacgg acauacugga uuuuaaccca aguuuggaga agcacgaacu cgagaaacag     1620

ccgauguacc aacuguggca uuuacuguau agcuacgagu ccgacaaguc uagaaccggc     1680

aaugaaagcc uacucaacaa gcuggaggcc aauuucgggu uuccccagga auaugcuuca     1740

guccuugccg augcaacauu cgaggacgau uacggaaaca ucaguguuaa agccauuagg     1800

aagaucuugc cucaucugca cgacggcuac gacuauuccg aggcuuguga acgggcgggu     1860

uacaaccaca gcgugcgcuc ucucacuaaa gaggaacugg cacagaaggu auauaaagau     1920

agauuagacc ugcuuccaaa gaacucauug aggaaucccg uggucgagaa gauucucaau     1980

cagaugauca acgugauaaa ugccaucauu guugaauacg ggaaaccuga ugagauccgu     2040

auugagaugg cucgggaacu gaagaguagc gccgcaaaac gagaggaaag aaccaagucc     2100

aucaaccaaa augccgcuga gaacaaaagg auaacagaaa uucuacagac ugaguuuggc     2160

cugucuuaua ucucgcgcaa ugacauugug aaguacaaac ucuaucagga gcuggaagcc     2220

aacggauuca agacccuuua cagcaauaca uacaucccaa aggagaaauu guuuuccaag     2280

gauuucgaca uagaacacau cguccccaaa gcacggcugu uugaugacuc auucggcaac     2340

aagaccauug aggcgagaga ugugaauuua gcuaaaagua acaagacugc cuucgacuuu     2400

guaaaggaaa aauaugggga gcagggaauc gaggaauaca agaaaaagcu cgauacgcug     2460

cuuaaagaga augcaauuuc uagcgguaag uauaagaaac ugaugauguc cgaagccgac     2520

aucccgucag auuucauaaa cagggacuug cgcaacacac aauacauugc ucgaaaggcc     2580

uaugagaucc ucggcgagcu gguuaaaacc aucacuccua ccacagggaa gauuaccgac     2640

cggcuacgug aagauuggca gcugaucgac gugaugaaag agcucaauuu ugaaaaguac     2700

gauagacugg gacuuacuga ggucauugaa gacagggaug gccgcaagau aaaacggauc     2760

aaggacugga caaaaagaaa cgaucauagg caccaugcaa uggacgcuuu gacgauugcc     2820

uucaccaagc ccagcuauau ccaguaccug aauaacuuaa augcgcgauc ugauaaaucc     2880

gguaguauuu acgccaucga gacuaaggag cuccaccgcg aaaacaauac acugcgguuu     2940

aacgcuccaa ugccucuuaa ugaguucaga aaggaagcaa aaaggcaucu gagcgccaua     3000

cuggugucua ucaaggcuaa aaacaagguc augacccaaa augugaacaa aauuaagacu     3060

aaaaauggga ucauuaagaa gguucagcuc acuccccgug gcgcccugca caacgagacc     3120

aucuauggaa cgaaaaugcg cccaauaauu aagaugguga aaaucggcgc agcguuggac     3180

gaagccacca ucaauaaggu auccucaccc acaauucggg aggcucuguu aaaaagacuc     3240

aacgaguaca guggggaugc caagaaggca uuugcuggaa agaacgccau aacuaagaau     3300

ccuaucuauc ugaacgcaga acacaccaaa acagccccag cuauggucaa gauuguggag     3360

ugggaaaugu acuauccgau caggaaacuu auuacucccg accugaagau cgagaagguu     3420

auagauaaug guaucaaaga aauuuugaag aaacgacuca aggaguucgg cggggacgcg     3480

aaaaaggccu uuagcaaccu ggacgagugc ccuaucuacc uagaugaaga gaagaauauu     3540

acccugaaac gggugucgau cgaaggaguc cuuuccgcaa uaccagugca uacgcucaag     3600

aaccacgcug gcaaagagau uaaggacgag gaugguaaag ccguacuggg ggacuaugug     3660

cagacaucua auaaccauca cguugccauc uaccaggaug cuaagggcaa uuugcaagac     3720

aacgucauca gcuucuuuga agcagccgag cgcaaaucac agaaugugcc cauuguggau     3780

aagaaccuga auagagacaa gggauggcac uucuuauaca ccaugaaaca gaacgaauau     3840

uuugucuucc cuaaugagga aacuggcuuc guuccaagug agaucgaucu cauggacgag     3900

aacaauuacg aaaugauauc ccccaaccug uuuagggugc agacaauguc ucggguggau     3960

uaugggaaga ccauuauccg agaguacgua uucagaaacc aucuugaaac uauucugaau     4020

gacaacuuga aacucaaggg acuggucuuu aaaaagauca agagccuaaa agcgcuggag     4080

ggcauaguga agguucgcgu gaaugcuauu gguaaaaucg ucgccguggg ggaauaugac     4140

uga                                                                   4143


<210>  3204
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3204
augaagaaaa aguacgccau cggcauugac auagggacca acagcguggg augguccguc       60

guuacagaug acuauaaagu gcccucuaag aaaaugaagg uauucgguaa uacugagaag      120

agauacauca aaaagaaccu gcucggcacg cuucuguuug augaagggaa uaccgcugag      180

aacaggcggu ugaaacgcac agcacgacgu agauauacua ggcggcgcaa uagaauuuua      240

uaccugcagg aaaucuucgc cgaggaaauu aacaagaucg acgauucauu uuuccaaagg      300

cuagacgaua guuuucucau aguggaggac aaacagggaa gcaagcaccc uaucuucggc      360

acccugcagg aggaaaagga guaucauaaa caguuuccaa caauuuacca ccuucggaag      420

caacuggcgg auuccucuca gaaagcugac auccgcuuga uuuaucucgc ccuggcacau      480

aucauaaagu accgagguca cuuccuguuu gaaggggauc ucaaaucgga gaauaaggac      540

guccagcauu uauucaacga uuuuguggag auguucgaca aaaccguuga aggaucauac      600

cugagcgaga aucuuccgaa cguggccgac gucuuggugg aaaagguaag uaaguccaga      660

caccuggaga auauucuaca cuauuuuccc aacgagaaaa agaauggccu cuucggcaac      720

uuccuggcuc uugcacuggg guugcagccu aauuuuaaaa cuaacuucga acucgccgag      780

gaugcuaaga uccaauuuuc uaaagaaaca uacgaggaag accuggagga auuacuggga      840

aagauuggug augacuaugc cgaucucuuc aucgcgacca agagccuuua cgacggcauc      900

cuguuggcag ggauucuauc cacgacuaac ucaaccacaa aagccccacu gaguagcucu      960

augguuaaua gguaugagga gcaucagaag gaucucgcuc ugcuuaaaaa cuuuauacac     1020

cagaaucugu ccgacucaua caaggaagug uucaacgaua aauugaagga cggauaugcc     1080

ggcuacaucg aggguaagac uacccaggaa aauuuuuacc gguucauuaa aaaggcaauc     1140

gagaaaauag augggagcaa cuauuuuauu gaaaagaucg agcgugagga cuuccuccgc     1200

aaacaaagaa cauuugacaa uggaaguauc ccccaucaga uucaccugag ggaaaugcag     1260

gcuaucauuc ggcgacaagc caaguucuac ccuuucuuag aggaaaacca ggauaagaua     1320

gagaaaaucc ugaccuuccg cauuccauau uacgucggcc cccuagcgag aggcaagucu     1380

gaguucgccu ggcucaauag gaaauccgac gaaaagaucc ggccuuggaa cuuugaugag     1440

augguggaca aagaaacgag cgcagagaau uucaucacuc gcaugacaaa cuaugaucag     1500

uaccugccag accagaaggu gcuuccgaaa cacucguugc uguaugaaaa guuugcuguc     1560

uacaaugagc ucaccaaggu uagauacgug acugagcaag ggaaaucuuu cuuuuucgau     1620

gccaacauga agcaggaaau uuuugacagg acauucaaag uauaucguaa ggugaccaaa     1680

gagaagcuga uggauuuucu uuccaaggaa uuugacgagu uucggauagu cgaucuguug     1740

ggacucgaca aagauaauaa gucauucaac gcuagucugg guacguacca ugaccugaaa     1800

aagaucguga gcgaagacuu uuuagauaac uccgagaaug aggacauucu agaaaacguu     1860

gugcucaccc ugacacuuuu cgaggaucga gaaaugauca gaaaaagguu ggagaaguau     1920

aaggacgucc ugacugagga acagcgcaaa aagcucgagc ggagacacua caccggcugg     1980

gggaggcugu cugccaaauu aauuaaugga auccgcgaua aggugacacg aaaaacuaua     2040

cuggacuauc uuaucgauga cggcaccagc aaccggaauu ucaugcaguu gauuaacgau     2100

gacacacucu cauuuguaga ugaaauccgu cuggcacaag guagugggaa ggccgaggac     2160

uacagagcug aaguucagaa ucuagcgggc ucccccgcaa uuaagaaagg aauccugcag     2220

agccucaaga uaguggauga gcugauugag gucaugggcu augacccuga acauaucgug     2280

gucgagaugg ccagggaaaa ccaguucacc aaucaagggc gccggaacuc ucagcagaga     2340

uacaaaaaga uugagaaugc uaucaaaaac cuugacucaa auuugaacag uaagauacug     2400

aaagaauacc caacuaauaa ccaggccuua caaaacgaua ggcucuuucu guauuaccuu     2460

cagaauggaa aggacaugua uacggaugag gagcuggaca ucgaucaguu gagccaauac     2520

gacauugauc acaucauucc ccaggcauuc aucaaggacg auucccucga caacaaagug     2580

cugacaaagu cugccaaaaa ucgagguaag ucggaugacg uuccuagccu agaaauagug     2640

uauaaaaaga agaacuuuug gaaacagcug cucgacuccc agcugauuuc acaacgcaag     2700

uucgauaauc uuaccaaagc ugagcggggc ggguugacua acgaagacaa ggccagauuu     2760

auccagaggc agcugguaga gacccgucag aucacaaaac augucgcacg cauuuuagau     2820

acccgguuca auacuaagcu cgacgaagcg ggaaacagaa ucagggaucc aaaggugaau     2880

auuaugacac ugaaaaguuc ucuuguuagc caauuucgaa aggacuacca gcuguauaaa     2940

gugcgggaga uaaacaauua ccaccacgcu caugaugccu accuaaacgc agucguggcc     3000

acggcucucc ugaagaaaua uccccaguug gccccggagu ucguauacgg cgacuauccu     3060

aaguacaauu ccuauaaguc acgcaaaagc gcaaccgaaa aggugcuguu uuacucuaac     3120

aucaugaauu ucuucaaaag aguuuuaguc uauuccaaga cuggugaggu gaggauucgg     3180

cccgugaucg aagucaacaa agagacaggg gaaauuguuu gggauaagaa gagugacuuu     3240

cgaaccguga gaaaagugcu cagcuaccca cagguaaacg ucgugaagaa aacagagguu     3300

caaaaucacg gccuggaucg cggaaagccu aaaggcuucu acaacgcuaa ucuuucuccc     3360

aggccaaagg agggguccgu ggaaaaccug guccccgcca aacagucauu ugacacuaag     3420

cguuauggag guuacgcggg caucaguaau agcuaugccg uguugguuaa cgggauaauc     3480

gagaagggca aaaagaaauc gaaggcugaa gugaccgagu uccagggaau uuccauccuc     3540

gcacggaaag auuuugacaa gaauccuaag caauaccugc uaaaccuggg uuauaaagac     3600

auuaagucua ucauaaaacu uccaaaguac agccucuucg agcuggaaaa ugggucaaga     3660

aggauguugg ccaguauucu guccacgaac aauaaacgcg gcgagaucca uaagggaaac     3720

gaaauguauu uaccggauaa guuuguaacc cuccuguacc acgcuaugcg agucaauaga     3780

acacuugagc ccggccacaa aaaguacgug gaagcccauc ggcacguguu cgacgagcug     3840

uugacuuaua uccucgagua caacgagaaa auugucgggg caaaggcgaa uggaaaaagg     3900

auaguugaag ccuauucugc ucgcaaggau accgacaacc uggaggaacu augcaauagc     3960

uuuaucaacc ugcuuaaccu cacagcccug ggcuccgcag uggauuucga auuuuugggu     4020

acuaagauuc cucguuacag agacuauacc ccaagcucau uacugaaagc uacgcucauc     4080

caucagagug uaacagggcu guacgagacc cggauugauc uuucuaagcu gggaggcgac     4140

uga                                                                   4143


<210>  3205
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3205
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggaugggc ccugguguac       60

gaguccgaaa acgcugauga gaagucuuca auagucagau uggguguuag ggugaauccc      120

uuaaguguag acgaaaaagg caacuucgag aaggggaaaa gcaucacuac gaaugcagau      180

cggcagcaaa cccacggagc ccgcauuaac cugcagcgau auaagcuacg uagagaaaau      240

cucaaggacu gccugcagaa acagggcugg aucgguacag aggcgcuuua cgaggaaggg      300

aagacuucca ccuuugagac auauaugcug agggcuaaag ccgcagaaga ggagauuucu      360

uugcaugaac ucugucgggu gcuguucaug cugaacaaga aacgcggaua caagucgaau      420

agaaaggcca acaauaaaga ggauggcaag cucuuugacg gcaugaccgu cgcuaaaaag      480

uuauaugaag aacaccugac uccugcagaa uacucacuua aauugcugag cagugggaag      540

aaauucaagc aagaguauua cagguccgau cuaaaggagg aauuugaccg gaucuggaac      600

gagcagcaga aauacuaucc agaaauacuc acagaugacu ucaagcagca acugcaggga      660

aaaucuaagg guaauaccag caaaaucuuu cuuggcaagu acgggauuua uuccgccgau      720

cugaagggau uggaccgcaa acuccagccg cugaaguggc gaguggaggc uuuaaaacag      780

aagguugaua aagaagugcu ggccuucguc aucucagacc ucaacggcca aauugcgagu      840

acgagcgguc uucugggggc aaucucugac agauccaagg aguuguacuu uaauaagcag      900

acugugggac aguaucuaug gaccucacug aaagagagcc cccauguaag uauaaagaac      960

aaaccuuucu acaggcaaga uuaucucgac gaauuugaga agauuuggga aacacaggcc     1020

acuuaccaca aagagcugac cccagaacuu aagcaggaga uccgggauau uaucaucuuc     1080

uaccagcguc cccugaaguc uaaaaagggc uugauuuccg uuugcgagcu cgaacaacgc     1140

aaagugaagg ucacaauaga cggcaaagag aaggaaauca gcauugggcc uaaaguggcu     1200

ccaaagucgu cucccauguu ucaggaguuc agaaucuggc agaaucugaa caacauauua     1260

cugauugaua agggaaauaa cuccaauagg ccucuauaug aggaagaacg gaaccuccug     1320

uacgaagaac uuucagugaa agaaaaguug aguaaagccg aggcacugaa gguccucaau     1380

aaaaacggua agcaguggga ccugaauuau aaggagaucg aaggcaaccg aacccaagcu     1440

guucuuuacg auuguuauaa ucgcaucauu gggaugacgg gacaugagga augcaacuuc     1500

aaaaagcuga aagccagcga caucagauac aaugugaaga cuauuuuuaa aaacuugggc     1560

uucuccacag auauacucga cuuugauucu agccugcagg gucacgaguu agaaaggcag     1620

ccaucauauc agcuguggca ccuacucuac aguuacgagu ccgacaauag ccggaccggg     1680

aacgacgcgc ugcuucgcaa guuggagacu gccuucucua ucccgaagga auaugcagcu     1740

guacugucag auguggucuu ugaggacagu uacggcaauc ucagcgugaa agccaugaga     1800

aacauucugc cccauuuaca agcuggacug gauuauuccc aggccuguga aaaggcaggc     1860

uacaaacacu cuaggcguuc gcuuacaaag aacgaguugg acaaaaagga auauaaggag     1920

aaacucgagc ugcuaccuaa gagcucccug cauaauccag uuguggagaa aauccucaac     1980

cagaugauca augucauuaa cgccgugaua gaugaguacg ggaagcccga cgaaauccgg     2040

auugagcugg cucgagagcu uaaaaucgga gcggcagaaa gagagaauau gaccagguca     2100

auuaaccagg guaaugccga aaacaagcgc auccgggaga uauuggaaaa agaguucgcu     2160

cugaguuaua ucucuagaaa ugauauuauc aaguacaagu uauacgagga acucaaaagc     2220

aacggcuuua agacgcugua uuccgacacc uacauuucaa aagauaaacu uuucagcaaa     2280

gacuuugaua ucgagcacau aauuccuaag gccaggcugu ucgacgauuc uuuguccaau     2340

aagacaaucg aagcacgcga cguaaacauu gccaaaagua auaagacugc uuuugacuuc     2400

aucagcgaga aauaugauca acaggggaua gaagccuaca agaaaaagcu cgacauccug     2460

cuacaggaug gaucuauuuc caaggcaaaa uauaaccgac ugcucaugaa ggaggcggac     2520

aucccaucag gcuuuauuga ucgggaccug cgugauaccc aauacaucgc uaaaaaggcc     2580

uaugagauac uuagugacuu gguuagaaau gugacaccca cuaccggugc agucacaagc     2640

aggcugcgcg aagauuggca guuaguggac gucaugaaag agcucaacuu cgaaaaguac     2700

cagaagcugg ggcuugugga gguuauugaa gaucgggacg gcagaaaaau caagaggauc     2760

aaagauugga ccaagcgaaa cgaccaccgc caucacgcca uggacgcucu gacuauugcc     2820

uucacgaaac cgucguacau ccaguaucua aauaaccuca augcacgguc cgauaagaac     2880

ucuagcauuu acggaauaga gaauaaggag cugcauagag aagacaacaa auugagguuu     2940

aaugcuccuc ugcccaucaa cgaguuccgu gccgaagcgc gccggcacuu aaagucaauu     3000

cucgugagug uaaaagccaa gaauaaaguc augacacaaa acgugaaugu uaucaagacc     3060

aaacauggcg ugauuaagaa gguccagcug acuccaagag gggagcuuca caacgcuacc     3120

uuuuauggaa caaagaagag gccuaucaua aaaaugguga agguaggugc agcccuggau     3180

gaggugacca ucaaaaaggu uuccucuagc uucauucgag aggcuuugcu cgaccggcug     3240

cacgaguaca auggcgaugc caagaaagca uuuacugggc gcaacuccau cgacaagaau     3300

cccauuuaua ucgaugagga gcauaaaaag acagucccag cgcuagugaa aacgauagaa     3360

ugggagaugu accaccccac cagaaagaug auuucaccug accugaagau cgauaaagug     3420

aucgacaagg gaauuaggga gauacuucgg acucgacucg ccaaauucaa cggcgaugcu     3480

cagaaggccu uuagcaaccu ggacgaaaau ccaaucuaua aagacgaggc aaagaagauu     3540

acauugaaaa gagucagucu ggaagguguu ucuuccguga ucccguuaca uaagcucaaa     3600

gauaugggcg ggaagcccgu gguaggaaaa gacggcaagc cugucugggg agauuacgug     3660

cagaccagca acaaucacca cauugcuuuc uacauggacg agaacgggaa ucugcaagau     3720

gacguuguga cauuuuucaa agccgcacgc aggaagucgc agggcguccc agugaucgau     3780

aagaacuaua aucaugacaa agguuggcac uuucuuuuca cuaugaagca gaacgaauac     3840

uuuguuuucc ccaaugaggc caccggguuc auaccuacgg agguggaucu gauggacgaa     3900

gcuaacuaug auaugaucuc accaaauuug uaccguguac agaaaauguc uaagaccaaa     3960

caaggaauua cagacaagcg ggauuauugg uuuagacauc accucgagac uguccugaac     4020

aaugaccuaa aggugaggaa caguaucuuc aaacagauua auucccugaa gccccuugaa     4080

ggcaucguga aaguccgcau aaacgcgauu gguaagaucg uugccaucgg cgaguacgac     4140

uga                                                                   4143


<210>  3206
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3206
augaagagaa uccugggccu cgaccuuggg accacaagca uuggaugggc ccuggugaac       60

gaggcugaau ccaauaacga ggcaucuuca auagucaggu uggguguucg ggugaauccc      120

uuaacuguag augaaaagag uaacuucgag aagggcaaag ccaucacgac caaugcggac      180

cgccagcugc gacacggggc ucguauuaac cuacaaagau acaagcucag gcggcagaau      240

cugcaugauu gccuucagaa acagggaugg cugggcacag aagccaugua ugaggagggu      300

aaggcaagca cuuuugaaac cuacaaguug cgcgccaaag cugcagagga agagaucucc      360

cuccacgagu ucgccagagu gcuguuuaug cugaacaaga aaagggggua uaagucuaau      420

cggaaagcua acaauaagga agacggacaa cucuucgaug gcaugacaau ugccaagaaa      480

uuauacgagg aacaucugac cccugcggag uauucgcuuc aguugcugaa caagggcaaa      540

aaguuuacuc agggguacua ucgcucagac cuaaaugcag aacucgagcg aaucugggau      600

gagcagaaaa aguacuaccc agaaauacug acagacgagu ucaaacaaca gcuugaagga      660

aagaccaaga cgaacacuag caaaaucuuu cuggccaagu augguauuua cagugcugau      720

uugaaaggcc ucgacagaaa gcugcagccg uuaaaaugga gggucgaggc ccugaagcag      780

caaguggaua aggaaguucu cgcauucgug aucuccgacc uuaaagggca gauugcuaau      840

accucuggac uguugggcgc caucagcgau cgguccaagg agcuauauuu uaacaaacag      900

acagucgguc aauaccugug ggcgucacuc gaggaaaacc cccacauaag uauuaagaau      960

aaaccuuucu aucgucagga cuaccuggac gaguuugaaa agaucuggga gacucaggcc     1020

accuuccaua agcagcuuac accagaacug aaacaagaga uucgcgauau caucauauuu     1080

uaucagagac cccucaagag caaaaagucu uugauuuccg ugugugagcu ggaacagagg     1140

aaaguaaagg caaccaucga cgggaaggag aaagaaauua cgaucggacc uaagguugcu     1200

ccaaaaucaa gccccguguu ccaggaguuc cggauauggc aaaacuuaaa uaacguccug     1260

cuaauugaua augacacuaa cgagaagcga ccucucgaug aagacgagcg cuaccugcuu     1320

uacaaagaau ugaguaucaa ggccaaacug ucuaagacag aggcucucaa gauccugaau     1380

aaaaagggca aacaguggga ucuuaacuau aaggaacugg agggcaauag aacccaggcc     1440

auuuuguuug acugcuacaa caggaucauu acucucacag ggcacgagga augugauuuc     1500

aaaaagauaa aggcauccga gauccggcac uaugugagca ccauuuuuaa aaaucuggga     1560

uucucgacgg aaaucuuaga cuuugaucca ucucuggaga agcaugaacu agagaaacag     1620

ccgauguacc aacucuggca ucugcuuuau uccuacgagu cagacaacag ucgcaccggu     1680

aaugagagcu ugcugagaaa gcucgaaaca acuuucggcu uucccgaaga guaugccacc     1740

gugcugugcg acgucguuuu cgaagaggau uacgggaacu uauccgugaa agcuaugagg     1800

aagauccugc cuuaccuuca ggcgggaaau gacuauucuc aggcaugugc cuacgcuggc     1860

uauaaccaca gccgucacuc auugacaaag gaggaacucg aucagaaagu auacaaggag     1920

cggcuggaac uacugcccaa aaacagucuc cgaaaucccg uggucgagaa gauucugaac     1980

caaaugauaa augugaucaa cgccauuauc gacgaguaug guaaaccuga ugaaauuaga     2040

aucgagaugg caagggaacu uaaguccagc gccgcugacc gcaagaaaac uacccacgcc     2100

auaaaucagg ggaacgauga gaaucagugc auccgggaaa uuuuggagaa ggaguuugca     2160

cugucuuaca ucucaagaaa cgacauuauc aaauauaagu uauacgagga acucaaacca     2220

aauggcuuca agacacugua cagugauacc uauauaccca aagacaaacu uuuuagcaag     2280

gauuucgaca uugaacauau cauuccgaaa gcgaggcugu uugaugacuc cuacucuaac     2340

aagacuuugg aggcucgcga caucaaucuc gccaaaucga acaagacggc auucgauuuc     2400

guuaaagaaa aguauggaga gcaaggcgcc gaggcuuaca agaaaaagcu ggacaugcua     2460

cuggggaaua acgugauaag caaaccuaag uauaauaaac uccugaugac agaagccgau     2520

auccccuccg acuuuauuaa ccgagaucuu cggaacaccc aguacaucgc acguaaggcu     2580

ugugagauuu ugggagaacu ggucagaacu gugaccccaa caaccgguaa gaucacuaau     2640

agguuacgcg aggacuggca gcucguagau guuaugaaag aacugaacuu cgagaaguau     2700

gagaaacuug gccugacaga aauaguggag gaccgggaug ggagaaagau uaaaaggauc     2760

aaggacugga cgaagcgaaa ugaucaccgc caucacgcca uggacgcgcu agccaucgcu     2820

uuuaccaaac cuucauacau ucaguaccuc aacaaucuga acgcacggag uaauaaggga     2880

gauucuaucu augccauuga aaacaaagag uugcauuacg aggaaggcaa gcugagauuc     2940

aaugcuccca uaccagucaa cguguuuagg gccgaggcaa aacgucacuu aagcgcgauc     3000

cucgucucca uuaaggccaa gaauaaagug augacucaaa acguuaauaa gaucaaaaca     3060

aagcacggua uuaucaaaaa gauacagcug accccacgcg ggccucuuca uaacgaaaca     3120

aucuauggca cuaaaaugcg gccaauuauc aagaagguga aaguaggagc ugcccuggac     3180

gaggcaacca uuaauaaggu cucaagcccg gcuaucagag aaguguugcu caaaaggcug     3240

aacgaguacu cuggcaacgc caagaaagca uucacgggga agaauacccu agacaagaac     3300

cccauauauc ugaaugccga gcgaacaaaa acuguuccug cuuccgugaa gaccgucgaa     3360

ugggagaacu accacccaau ucggaaaccc aucacaccug aucuuaaggu ggacaaagua     3420

aucgauaagg gaauugguga aauacucaag gcgcgccugg aggaauuuaa uggcgacgcc     3480

aaaaaggcau ucaguaacuu ggaggagaau ccaaucuauc uggaucagac uaaaaagauu     3540

gcuuuaaaaa gagugagcau cgaagggguu cucucuacca uuccccugca uacgcuuaag     3600

aaccaggccg gaaagccgau caugggcaaa gacgguaagc cuguccuggg gaauuacgug     3660

caaacaucca acaaucacca cauagccuuu uacuaugaug aggacggcaa cuugcaggau     3720

aaugcugugu cauucuuuga agcagccgag aggaaaaguc agggaaucag cgucauugac     3780

aaggauuaca accgggacaa aggcuggcga uuccucuuua ccaugaagca gaaugaauau     3840

uucguuuucc caaacgaggc gacuggguuu auccccucgg agguggaccu gacagaugaa     3900

gcuaauuacg gaauuaucuc cccuaaccua uauagagugc aaaagauguc ucgcauagag     3960

aagggcacca gcguaucaag ggacuacugg uuccgucauc accuggaaac uauucuuaac     4020

gaugacaguc ggcucaaaaa uuccgccuuu aaaagaauca acucucugaa gcccuuggag     4080

gguaucauua aagucaggau caauagcaua gggaagauug uggcaguugg agaguaugau     4140

uga                                                                   4143


<210>  3207
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3207
augaagaaag gcuacagcau cgggcuggac auuggaacca acuccguggg uuucgccguc       60

auaacagaug acuauaaggu ucccucuaaa aagaugaaag ugcucggcaa uacugauaag      120

agauuuauca agaaaaaccu uauuggggcu cuguuguucg acgagggaac gaccgcagaa      180

gccaggcggu uaaagcgcac agcgcgacgu agauacacua ggcggaaaaa ucgccugaga      240

uaucuacagg agaucuuuuc agaagagaug aguaagguag auagcuccuu cuuucacagg      300

cucgacgauu cuuuccugau uccugaagac aaacgggagu cgaaguaccc aaucuuugcu      360

acccuugagg aagagaagga auaucauaaa aaguucccga caauauacca ucugcgcaaa      420

cauuuggccg auucaaagga gaaagcagac cuccgacuga ucuaucuggc ccucgcucac      480

augauuaagu acagaggcca cuuuuuauac gaggauaccu ucgacaucaa aaacaaugau      540

auucaaaaga ucuuuaacga guucauaagc auuuaugaca augcauuuga agguaguucc      600

cugucugggc agaacgccca gguggaagcu aucuucacug acaagauuag caaauccgcc      660

aagagggagc ggguccuuaa auuguucccc gaugaaaagu caacaggacu guuuagugag      720

uuccuaaaac ucaucguggg caaucaggcg gacuuuaaga agcacuucga ucuggaggac      780

aaagcaccuc uucaauuuag caaggauacc uacgacgaag aucuggagaa cuugcucggc      840

cagaucgggg acgauuucgc cgaccuguuu guugcugcca aaaaguuaua ugaugcaauu      900

cugcucucug gaauacuuac ggugacugac ccauccacca aagcuccccu gucagccagc      960

augaucgaac guuacgagaa ucaccagaac gacuuggucg ugcuaaagca guucauuaag     1020

aauaaccugc cugaaaauua uguagagguu uuuagugaug acucuaaaaa cgguuacgcg     1080

ggcuaugugg gggucgauuc caagauccgc aaaagaaacg ccaagcucgc aacagaggaa     1140

gaguucuaca aauaccugaa gaauauacuu aacaauguga agggagcuga cuauuuucug     1200

gauaaaauug aaagggagga cuucuugcgg aagcaacgaa cuuuugauaa cggcagcauc     1260

ccacaucaga uccaccucca ggaaaugaau gccauucugc gcagacaggg ugagcauuac     1320

cccuucuuaa aagagaacag ggaaaagauc gagaaaauuc ugaccuuccg gauaccuuau     1380

uacguggggc cacuagcucg cggaaauaga gacuuugccu ggcucacaag gaacucggau     1440

caagcaaucc guccguggaa uuucgaagag auugucgaca aggccucuuc cgcugaggau     1500

uuuaucaaca aaaugaccaa uuaugaccug uaccuucccg aagagaaggu uuugccuaag     1560

cacucacugc ucuaugaaaa auucgcggug uacaacgagc ugacgaaggu aaaauuuauc     1620

gcagaaggcc uucgggauua ccaguuccug gacaguggcc agaagaaaca aauugugaau     1680

caguuguuua aggagaagcg aaaagucacu gagaaggaca uaauccacua ucuccauaca     1740

guggaugggu acgacggaau ugaacugaaa gguaucgaga agcaguucaa cgccagcuua     1800

uccaccuauc acgaucugcu aaaaauuauc aaggacaagg aauuuaugga ugacccaaag     1860

aaugaggaaa uacucgagaa caucguucau acucugacaa uuuucgagga uagagaaaug     1920

aucaagcaga ggcuugcuca auacgacucu uuguuugaug agaaagugau uaagagccug     1980

acccgccggc acuauacggg cugggggaaa cucucagcca ugcugaucaa cggaauaaga     2040

gacaagcaga ccggcaagac aauuuuagau uaccugaucg acgaugcaua uuacaauagg     2100

aacuucaugc agcuuauuaa ugacgacggu uugaguuuua aagaaaucau acagaaggcc     2160

caagucgugg ggaaaacuga ugacguaaag cagguugugc aggagcuccc cggcuccccu     2220

gcuaucaaaa aggggauucu gcagagcauc aaaauugucg augagcuagu gaagaucaug     2280

cgccauaacc cagaaucuau agucauugag auggcccgag aaaaucaaac cacagcacgg     2340

ggcaagaaaa acucacagca gcguuacaag agaaucgagg acgcgcugaa aaaucucgcu     2400

cccgggcugg auaguaacau ccuuaaggaa cacccgacug acaaugugca guugcaaaac     2460

gauaggcugu ucuuauauua ccuccagaau ggaaaagaca uguauaccgg ugaggcccug     2520

gauauuaacc agcuuagcuc cuacgacauc gaucacauua uaccucaagc auucaucaag     2580

gacgacucuu uugauaaucg cguucugaca ucgagcaagg acaaccgggg caaauccgau     2640

aaugugcccu caauugaggu aguccagaag agaaaagccu ucuggcagca guugcucgac     2700

aguaagcuga ucucugaaag gaaauuuaac aaccuaacca aggcugagcg agggggacug     2760

gaugaacgcg acaagguggg cuucauuaaa cggcaacucg uugagacuag acagaucacg     2820

aagcaugugg cccagauacu ggaugcaagg uauaauacag aagucaacga gaaagacaag     2880

aaaaaucgua ccgugaagau cauuacucuu aagagcaacu ugguauccaa uuuucgcaaa     2940

gaguuccggc uguacaaggu gagagaaauc aacgauuauc accaugcuca cgacgccuac     3000

uuaaaugcgg uugucgccaa agcuauucuc aagaaauacc caaagcugga gccugaauuu     3060

guguauggug aguaccagaa guaugaucuu aaaagguaca ucucacgaag caaggacccc     3120

aaagaagugg agaaggcaac cgagaaauau uucuuuuacu cuaaccugcu aaauuucuuu     3180

aaggaagagg uccacuaugc cgaugggaca auaguuaaac gggaaaacau ugaguacucc     3240

aaggacaccg gcgaaaucgc uuggaauaag gagaaagacu ucgccaucau uaagaaagug     3300

cucagucugc cacaagugaa cauaguaaag aaacgcgagg uccagacugg aggcuucagc     3360

aaggaaucua ucuugcccaa gggaaauucc gauaaacuga uuccuagaaa gacaaaagac     3420

aucuuauggg auacgaccaa guacggaggu uuugacucac cagugauugc auauaguauc     3480

cuccugauag cggauaucga gaaaggcaag gccaagaaac uuaagacugu uaaaacacug     3540

guggggauua ccaucaugga aaaggcugcc uucgaggaca acccgauuac auuuuuggag     3600

aauaaaggau accauaacgu caggaaggaa aacauccucu gccugcccaa guauagccua     3660

uucgagcugg aaaauggccg gcgaagacuu cucgcaucgg cuaaagagcu gcagaagggu     3720

aacgaaauag uguugccugu uuaccugacu accuuacucu aucacuccaa aaaugugcau     3780

aagcuggaug agccagggca ccuugaguac auucagaaac aucgcaacga auuuaaggac     3840

cuguugaauc ucguaucuga guucagccaa aaauaugucc uggccgaugc aaaccuagaa     3900

aagaucaagu cacuguacgc cgacaaugag caggcugaua ucgaaauucu ugcgaacagu     3960

uuuaucaauc uccugacguu caccgccuug ggcgcacccg cugccuuuaa auucuuugga     4020

aaggacauag auaggaaacg uuacacaacu guguccgaga uuuuaaacgc cacccugauc     4080

caccagucua uuacaggccu cuaugagacu cggaucgacc ugagcaaacu uggggaagac     4140

uga                                                                   4143


<210>  3208
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3208
augaaccuga gacacguggg caauuacaac aucgggcucg acaugggaac cgccagcguc       60

gguugggcug caauugauga gcagggcgaa cuucugcauu ucaaggggaa acccacaugg      120

ggauccaggu uguuugacuc ugccaauacu gcggcuacgg cccggaugcc ucgcggccaa      180

cgacguagau auauaaggcg gcgcuggaga uuagaucugc uacagaaguu cuuuucagag      240

gaaauggcag agaaagaccc agaguucuuu aucaggcucc ggcagaguag ccuguggccg      300

gaagaucgcg agggugggca caccgccuac cauuggcccc uuuucaacga cuccgauuuu      360

aaugaaaagg aguauuacga aaaauucccu acaauuuuuc aucugcgaca gugguugaug      420

gagacugacg agaaggcuga uaucagacuc auuuaucugg cauuccacaa caucguuaag      480

cacaggggaa auuuucugca agaaaacaau gacucucucu cggcccagaa cgcuaauaug      540

cgggagucau uagagagcuu cugcauagcc cuggaagaau ggugugauga gaaagagcuu      600

accugcaguu gugacccaca gaaguuggaa caccuguuua aagaggugaa guaccguucc      660

gauauccgcg acggcuucgc ggcacuacuc ggccuggccu gcucugggga aaacgaggua      720

gacgugaaag cuauggccaa ggaaauggga aaggcaauug ucgguuauaa agcugauuuc      780

agcaagaucu uuacagagug ugacaaagag ggcuccucau ucagacuuag ugaugacgaa      840

gggcccgagg ccuuucugga uauuugcccu gacgauggaa ucgcguuguu cgaagccaua      900

cagauggugu acagcucuuu uauucucacc ggcaugcuga cugagaaggg uuuaucccug      960

ucaaaaauca gcgcauacaa ucaauauggg caggaccucg aaagucuuaa ggcucugguu     1020

aaagaguacg ugccaaacga guauucuucc uucuuuaggg gacccacaua cagcggcucg     1080

accgaauaug auccuucuaa ggcccagggc uacacgcggu auaauuuggg gccauccaag     1140

aaaggaucag gugcuaagcc gauggccuac gaggacuuca aaaagagugu cgaacagcua     1200

uuugcaaaca cuaacgccau uaaugaugag cgauacaaag agaugauggg ccgcuucgcu     1260

gaagagaagu uucugagaag gcuccggacc agcgacaacg ggcgcauccc cuuccaacug     1320

aaucuugaag agaugacagc gaucauuaua aaccagggaa aguauuaccc uuuucuguug     1380

gaacagcaag agaaacucga gucccuggug agauucagga ucccauauua cguaggcccc     1440

uuaacucgua uuaaugcacg gcuggacucu aaggguaacg ccuguuucgc uuggagcaaa     1500

cgaaaggaug ggauggacuc agccaguauc aaaccuugga auugggauga aguuauagac     1560

aagcaugcau ccgcccaggc uuuuauugag agaaugaccg gcacaugcac cuaucuaagg     1620

ggagaaccag ugcucccccg cuguagccug cuuuacgagg aauauugcgu cuugaacgag     1680

cugaauggcg cccgguggac gcaggauggg gacaaguuca ucagauuuga uuacaaagac     1740

aggucugaua ucauugagga acucuucaag aaacgccgag gacggaucca guacgcagcg     1800

guggcugacu ggaugaagca agagcguuca cacgccaacg ugcaugucag ugguggccag     1860

ggggaaaaug cauuugagag cagacugucc ucuuaucacu ucuuuuguaa cgauguuuuc     1920

ggaguggacg aacuuccggg cucguacgua gauauggugg aggagauuau acuguggagc     1980

acuuuguuug aagacagguc cauccuccgc gaggaaauuc agcggaaaua uggugacguc     2040

cugaaugagg agcagaucaa gacaaucugc aagaaaagau ucucagggug gggcagguua     2100

agucgaaagc ugcuaugcuc ucucaaagcc caaaccgaua acggaccuaa gagcauuaug     2160

gacauacugc aggaaggcga ucagaauaac gggcgcgugg gacgggcuau gguuuuucag     2220

gagauccuuc acgacgaaca auuggguuuc gagcagaaaa uugaagagga gaacacucag     2280

uccaugcugg aaggcgagaa ucuccugauc gaagauuuac ccgggucacc agccauuaga     2340

aggagcauca accaggcagu gcguauaguc gacgagaucg ugggaauugc uggccaugcc     2400

ccugauaaua ucuuuauuga aguaacacgc gaggacgaua ccaagaaaaa ggguaagcgg     2460

acgaccacaa gauacucuaa cauccaaaaa gcgcuggagg cccuuaagga agagggggcu     2520

gaagcauuga gggagcucaa aacuuauaag cacaaugaac uggacaaacg acuaucccug     2580

uacuucaugc agaacggcaa gagucucuau agcggaauac cccuggauau ucggcgccuu     2640

ucugaguacc agaucgacca uauuauccca caauccuaca uaaaggauga cucauaugag     2700

aauauggccu ugguucugcc cggcgaaaac cagaguaaag cugacaauuu acucaucagc     2760

caggauauua gaaucaagau gaaagccacc uggacagcac ugcacaaggc ggggcuuaug     2820

ggagacaaaa aguacaacaa ucugaugaac ucguccauuu cugaugccaa gaugaaaggc     2880

uucaucaaua ggcaguuggu ggagacuagc caaucaguca agcucaccca gaugaugcug     2940

agucagaaau auccuggugc uaaggugcgg ccaauaaaag ccuccauuuc uagccagcua     3000

cgagacgcau gugaucuggu caagugcaga gaagcuaacg acuaccauca cgcccacgau     3060

gcauaucucg ccugucaagu ggggcgcuuu guucugaaga ggcauuccuc aauguucgac     3120

aauccgaucg gaaugacaaa agugauccgu gauuuuguaa agcggcaggg ccaggaguuc     3180

aaaagaaacg guaaacuucc cgcuuacgcg agcaggagug gcuuuauugu ccagucuuuc     3240

augcgcuccg gguuugacgc cgagaccgga gaauugcugc gagaugacug gaacgcaccu     3300

uucgagaucg cuagaauuaa acggugcuua aauuauaggg acuguuacau aagccgcaug     3360

ccaauggaag auucgggcgc cuuuugggac gccacuaucu acucacccaa ggcugggaaa     3420

aagggagcac ucccugugaa gcaaggccug ccaguugaga aauaugguuc uuacagucgu     3480

gaacaguucg ccuacuuuuu cguguauaag gcguauaacc ccaaaaagaa ggcuuacaga     3540

cuugaguucg aggccguccc ggugcgggua gcauccgccg uggaaacgag ggagaaugcu     3600

cuggaagagu augcaaagag cguugccaag caggcggggc uagaauuugu cgagguggcu     3660

cgcccuaaag uguacaagua ccaacucauu gagcugggag aagaucgauu guauaucaca     3720

ggcaaaagag agguccggaa cgccucucag cuggcauuca gcuuagacga aauugaucuc     3780

cugaagaggc uugccgaggg ugaagagacc uccucagagg aacgcgagag acuguuugac     3840

guuuugaucg aaaaauacgc uagguaugcc ccacggcucg cacgucagcu gaguguggau     3900

agcaugucug cuacuuucga gacccuaaau gccgaggaac agcugggcau acuucucucc     3960

cugguggaga ucgcgaacgg gaagcgcaau augauugacu ugucaagugc cggaggcagc     4020

aagaacgcag ggugcaugaa ucccacauuu uccucugaac ugucgagccc uggaucaaac     4080

uucaccuuua ucgaucaauc cguaacuggc auguucgagc gaagacagag guuagaacuc     4140

uga                                                                   4143


<210>  3209
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3209
auggugaaga aaaaguacgc caucggcauu gacauaggga ccaacagcgu cggauggucc       60

aucguuacag augacuauaa agugcccucu aagaaaauga agguauucgg uaauacugag      120

aagagauaca uuaaaaagaa ccugcucggc acgcuucugu uugaugaagg gaauaccgcu      180

gagaacaggc gguugaaacg cacagcacga cguagauaua cuaggcggcg caauagaauc      240

uuauaccugc aggaaauuuu cgccgaggaa aucaacaaga uagacgauuc auuuuuccaa      300

aggcuagacg auaguuuucu caucguggag gacaaacagg gaagcaagca cccuauuuuc      360

ggcacccugc aggaggaaaa ggaguaucau aaacaguuuc caacaaucua ccaccuucgg      420

aagcaacugg cggauuccuc ucagaaagcu gacauucgcu ugaucuaucu cgcccuggca      480

cauauaauua aguaccgagg ucacuuccug uuugaagggg aucucaaauc ggagaauaag      540

gacguccagc auuuauucaa cgauuuugug gagauguucg acaaaaccgu ugaaggauca      600

uaccugagcg agaaucuucc gaacguggcc gacgucuugg uggaaaaggu aaguaagucc      660

agaaggcugg agaauauccu acacuauuuu cccaacgaaa agaagaaugg ccucuucggc      720

aacuuccugg cucuugcacu gggguugcag ccuaauuuua aaacuaacuu cgagcucgcc      780

gaggaugcua agauucaauu uucuaaagaa acauacgagg aagaccugga ggaauuacug      840

ggaaagaucg gugaugacua ugccgaucuc uucaucgcga ccaagagccu uuacgacggc      900

auucuguugg cagggauacu auccacgacu gauucaacca caaaagcccc acugaguagc      960

ucuaugguua accgguauga ggagcaccag aaggaccucg cucugcuuaa aaauuuuauc     1020

caucagaacc uguccgauuc auacaaggaa guguucaaug acaaauugaa ggauggauau     1080

gccggcuaca uugaggguaa gacuacccag gaaaacuuuu accguuucau caaaaaggca     1140

auagagaaaa uugaagggag caauuauuuu aucgacaaga ucgaccgcga ggauuuccuc     1200

agaaaacaaa ggacauuuga caacggaagu auuccccacc agauccaucu gcaggagaug     1260

cacgcuauua uacggcgaca agccgaguuc uacccauuuu uagucgagaa ucaggauaag     1320

aucgaaaaga uucugaccuu ccgcaucccu uauuacgugg gcccccuagc gagaggcaaa     1380

ucugaguucg ccuggcucaa caggaagucc gacgagaaaa uccggccuug gaauuuugau     1440

gaaauggugg acaaggagac gagcgcagaa aacuucauua cucgcaugac aaauuaugau     1500

agauaccugc cagaccagaa aguccuuccg aagcacucgu ugcuguauga gaaauuugcu     1560

guuuacaacg aacucaccaa ggugaaguuc auagccgagg ggaugaggga uuaccaguuu     1620

cuggacucug gacagaaaaa ggauaucgua aaaacucuuu ucaagacaaa acguaaggug     1680

accgcuaagg acauuaaagc cuaucuggaa aauuccaacg guuacgcagg cgucgaauug     1740

aaggggcucg aggaacaauu uaaugccuca cugcccacgu aucaugauuu acugaaaauc     1800

cuacgggaca aggcuuucau ugacgcggag gaaaaccagg agauccucga ggauauagug     1860

cugacccuua cauuguuuga agaccgagag augaucagaa aaaggcugga aaaguacaag     1920

gauauucuca cugaggaaca gcgcaaaaag cuggagcgga gacacuauac cggauggggc     1980

agguuaagug caaaacugau caacgguauu cuugacaagg uuacacgcaa aacuaucuug     2040

ggguaccuca uagaugacgg caccagcaau cgaaacuuca ugcaacugau uaaugaugac     2100

acacuauccu uuguggauga gauccggcug gcccagggau cuggcgaagc ugaggacuau     2160

cgugccgaag uccagaaccu cgcagggagc ccugccauua agaagggaau ccugcaguca     2220

cuuaaaauag uggaugaguu gaucgaggua auggguuacg aaccagagca uauuguugug     2280

gaaauggcua gagagaauca auucaccaac cagggcaggc gcaauaguca gcagcggauc     2340

aacagacugg aaaaugccuu aaaggacuuu ggguccaaaa uucucgagga gcaaaagccc     2400

uacggaguca gcaaagugaa caauauccau cugcagucug auaggcuuuu ccuguauuac     2460

uugcagaacg gcaaggacau guauacugac gaggaacucg auauagacca ccugucagaa     2520

uacgauauug accacaucau cccucaagca uucauuaaag augacagucu agauaauaag     2580

guccugacaa agagcgcgaa aaaccgaggu aaguccgacg augugccauc uauggagauc     2640

guucacaauc gcaaaaacuu uuggcggcag cuccugaagu cgaaacuuau uagccagaga     2700

aaguucgaca acuugacgaa ggcugaacga gggggccuga ccaaugagga uaaagcccgu     2760

uuuauacagc gccaauuagu ggagacucgg cagaucacca agcauguagc aagaaugcuc     2820

gacacaaggu ucaacaccaa acuggacgaa gccggaaaua ggauucggga ucccaagguc     2880

aacaucauua cucuuaaauc caaucuggug ucacaguuuc gcaaggacua ucagcuauac     2940

aagaucagag agauaaacaa uuaucaccau gcucacgaug ccuacaucaa cgcaguugug     3000

gcuacagccc uccugaaaaa guacccgcaa uuggcgccug aguucgucua uggcgaauac     3060

cccacguaua auaguuacaa cucuaggaaa agcgccaccg aaaaggugcu guuuuauucc     3120

aauauuauga acuucuuuaa acggaucgua gugcacucaa agacugggga gguucgaauu     3180

agaccaguca ucgaggugaa uaaggaaaca ggagagauag ugugggacaa aaagagcgau     3240

uuccgcaccg ucaggaaagu uuuaucuuac ccucagguga acgugguaaa gaaaacagaa     3300

guccagaauc auggucucga ccguggcaag cccaaggggu uuuauaacgc uaaccugucc     3360

ccacggccca aagagggaag uguggagaau cuuguuccug caaagcagag cuucgauacu     3420

aaaagauacg gcgguuacgc cgggauuucu aacuccuaug cugugcuggu caauggcauc     3480

aucgaaaagg gcaaaaagaa aucaaaggcc gaggugaccg aguuccaagg aauuaguaua     3540

uuggcaagga aggacuuuga uaaaaaccca aagcaguacc uccugaaucu aggguauaaa     3600

gacaucaaga gcauuaucaa acugccgaag uacucgcuuu ucgaacucga aaacggaucc     3660

cgccgaaugc uggcgucuau uuugagcacg aauaacaaga gaggcgagau ccacaaaggu     3720

aaugagaugu aucugcccga uaaguuuguu accuuacucu accaugccau gcgggugaac     3780

aggacacugg aaccugggca caaaaaguau guagagacuc aucgccacgu cuucgacgaa     3840

cuucugauau acaucuugga guacaaugag aaaauugugg gagcuaaggc caacggcaag     3900

cguaucgugg aagcauauuc agcuagaaaa gauaccgaca aucucgagga acugugcaac     3960

aguuuuauua aucuacugaa ccuuacagcc cucgguuccg cagucgacuu cgaguuucug     4020

ggcacuaaga ucccacggua cagggauuau acccccucua gcuuguuaaa agccacgcug     4080

auacaccagu ccguuacagg gcucuacgaa acccgcauug accugucaaa acuuggaggc     4140

gauuga                                                                4146


<210>  3210
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3210
augaacuacg ugaaggagua ucacaucggc cuggacauug ggaccagcuc cauaggaugg       60

gccgucacag auucucaguu caaacucaug agaaucaagg guaaaacugc uauuggcguu      120

aggcuuuuug aagaggggaa gacggcagcc gaacggcgca ccuuccgaac aacucguaga      180

aggcugaaac ggcgcaagug gagauugcau uacuuagacg agaucuuugc gccccaucug      240

caagaagugg augagaauuu ccuaaggcgg cucaagcagu caaacauuca cccugaggac      300

ccagcuaaaa aucaggccuu uaucggaaag cugcuuuucc cggaucuguu gaaaaagaac      360

gaacgcggcu aucccacccu cauaaaaaug cgagacgagc ugccuguaga acagagagca      420

cacuacccag ugacaaauau cuauaagcug agggaggcca ugauuaacga ggaucggcaa      480

uuugaccucc gugaagucua cuuagcugug caucacaucg uuaaguaucg cggucacuuc      540

cugaauaacg caagugugga uaaauuuaag gucgggagaa uugacuucga uaaaaguuuu      600

aaugugcuua acgaggccua cgaagaguug cagaauggag aaggcuccuu caccaucgag      660

cccucuaagg uagagaaaau aggccagcug cuacucgaca cuaagaugag gaaacuggau      720

cggcagaagg cuguugccaa guuucuugaa gugaaagucg cggacaagga ggaaacaaaa      780

cgaaacaagc aaauugcaac cgccaucucg aaacuggugu ugggguacaa ggcugacuuc      840

gccacggugg caauggcuaa uggaaacgag uggaagauug aucucucaag cgaaacuagc      900

gaggacgaga ucgaaaaauu ccgcgaggaa cuguccgaug cccagaauga caucuuaacc      960

gagauuacau cucuguuuag ccagauaaug cucaacgaaa ucgucccuaa cgguaugucc     1020

auuucagaga guaugaugga uagauauugg acucaugaga ggcagcuugc ggaaguuaag     1080

gaguaccugg ccacccaacc agcaagcgcu cggaaagagu ucgaccaggu guauaauaag     1140

uacaucggcc aggcccccaa agaaaagggg uuugauuugg agaagggacu caaaaagaua     1200

cugucuaaaa aggaaaacug gaaagagauu gacgaacucc ugaaggcugg cgauuuccuu     1260

ccuaagcaac gcacauccgc caauguagug aucccacacc agaugcauca gcaggagcug     1320

gacagaauca uugaaaaaca agcaaaguau uacccguggu uggccaccga gaaccccgcu     1380

acgggugaga gggaucguca ccaggcgaaa uaugaacucg accagcuggu cucauuucgg     1440

aucccuuacu acguggggcc auuaguuacu cccgaggugc agaaggcaac aagcggagcc     1500

aaauucgcuu gggccaagcg aaaagaagac ggcgagauua ccccuuggaa ucugugggau     1560

aagauagaca gagcagaaag ugccgaggcu uuuaucaaga ggaugacugu caaagauaca     1620

uaucuacuca acgaggacgu gcugccagcc aauucucuuu uguaccaaaa guauaacgua     1680

cugaaugaac ucaacaaugu ucgcgugaac ggccggagac uguccgucgg gauuaaacag     1740

gauaucuaca gcgagcuuuu caagaaaaag aagaccguga aagcaucgga cgucgcgucu     1800

cuggugaugg cuaagacgag gggaguuaau aaacccuccg uggaagguuu gucagauccg     1860

aagaaauuua acaguaaucu cgccaccuau cuggacuuaa agagcaucgu aggcgauaag     1920

gucgacgaua acugcuacca gacagaccug gagaacauua uagaauggcg cuccguguuc     1980

gaggaugggg agaucuuugc agacaaacua acugaaguug aguggcucac cgacgaacag     2040

cgaucugccc uggugaagaa acgguauaag ggauggggcc gucuuagcaa aaaguugcug     2100

acagguauug ucgaugagaa ugggcaaaga aucauugacc ucauguggaa cacugaucag     2160

aauuucaugc agaucgugaa ccagccugua uuuaaggagc aaauagacca gcugaaucag     2220

aacgcuauca ccaaugaugg caugacauua agggaacgcg uggagucagu ucuggacgau     2280

gccuacacca guccccaaaa caaaaaggca auuuggcagg ucgugcgggu ggucgaagac     2340

aucguuaaag cugugggaaa ugccccaaag uccauuagca ucgaguucgc gagaaacgaa     2400

ggcaauaaag gggagauaac uaggucucga cgcacgcagc uucagaaguu guucgaggau     2460

caagcccacg aacucgugaa agacacauca cugaccgagg aacuagagaa ggcucuggau     2520

cucagugacc gguacuaucu guacuuuacu cagggaggua aggauaugua uaccggcgac     2580

ccuauuaacu ucgacgaaau cagcacaaaa uacgauauug accauauccu uccccagucc     2640

uuuguaaagg augacucuuu ggauaauaga guccuggugu cgagggcaga gaacaauaaa     2700

aagagcgacc guguuccagc caaauuauau gcugccaaga ugaagcccua cuggaaccag     2760

cuccugaaac aagggcuuau aacccagcgc aaguucgaga accugacuau ggauguggac     2820

cagaauauca aauaucgguc cuugggauuu gucaagagac agcucgugga aacaaggcaa     2880

guuauuaaac ugacggcaaa cauccuaggc ucaauguacc aggaggcggg uaccgauauu     2940

aucgaaacuc gagccgggcu gacaaagcag cuccgggagg aguucgaccu gccuaaggug     3000

cgcgaaguaa augauuacca ccaugcuguc gacgccuauc uuaccacauu ugcaggccaa     3060

uacuugaaca gaagguaucc aaaacugcgg aguuucuuug uguacggaga guauaugaag     3120

uucaaacgag gcucugacuu aaagcucaga aauuuuaacu ucuuucacga acugauggag     3180

ggggauaaaa gccagggaaa gguggucgac cagcagacug gugaacuuau aaccacgcgc     3240

gaugagguug cuaaguccuu cgacaggcug cuaaauauga aauacaugcu cgugucaaag     3300

gagguacaug aucguagcga ccaacuguau ggcgccacca uugugacagc aaaagaaucu     3360

gggaaguuga cuuccccgau cgagaucaaa aagaaccggc ccgucgaucu guacggagcc     3420

uacaccaaug gcacaagugc uuucaugacu auuaucaagu uuaccgguaa caaaccuaag     3480

uauaaaguga uugggguucc aacgacaagc gcggugggcu uaaagagagu cggaaaaccc     3540

ggcucugaau ccuacaauca ggagcuccac aggauaauca agucaaaccc uaaagugaag     3600

aaggacuucg aaauuguugu gccacacguc aguuauagcc agcugaucgu agauggggac     3660

uguaaauuua cccuugccuc ggauacuuac cagcaucccg caacacaacu gguguugucc     3720

aaggagucua uggagauuau cgcugacgau uucaaauuug uuaaggaaaa uccggccacc     3780

gccgacgagc agcucgugcg cgucuucgac gaaauaguga accagaugaa ucacuauuuu     3840

acuaucuucg aucagcgaag caacagacaa aaaguaacca aggcucggga caaguuugug     3900

ucacugccua cagagaguga auacgaggga gccaaaaaga cgcaggucgg caaaaccgag     3960

guuauuacua accuacugau gggucuucau gcaaaugcgg cucaggggga ucucaaggug     4020

cugggauugu ccacauucgg cuuuuuccag ucuagcggug gccugaacuu auccgaagac     4080

gccaugaucg uguaucaauc accaaccggg cuauucgaga ggcgcauuug ccugaaagau     4140

aucuga                                                                4146


<210>  3211
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3211
augaagcaga uccugaaaaa ggugcaaggc uacaacauug ggcucgacau aggaaccaau       60

agcgucgguu ggguugugac agaugagaac gccaaucuuc ugcaguucag gaaaaagaac      120

auguggggcg cuagauuguu ugaaaaaggg gacacugcac agggacggcg cuuaucccga      180

ucuacgcgua gaagguauga ucggcgcaga cagaggaucc acuaccugca agagaugcua      240

gccaccaugg uacuccccau ugacgaauca uucuuuaucc ggcugaauga ggcguuccuu      300

uggaaggaag auaagagugu gcagcgcgac uauauucugu uugcugauaa acaguucaca      360

gaccgagauu uuuacaagca guucaaaacu aucuaucauu ugagaaaguu ucuccuggac      420

accaaagaga aggccgaucc uagguucaua uaccuggcac uccaccauau gaucaaguau      480

cggggcaacu uuuuauacca aggucagagc uucgacgucg uggagaaugu ucaggaaucc      540

uuucgugugc ugcuugagga aauguacgag caguugggga aagaguggga auauggagag      600

cacuucgaug acauucaacg cauccuggaa aacacaacca uuggcaagaa aucuaagcag      660

gagcagaucg ccgcuauacu aacugaacaa uacggcaaua aaaagcucgc aaacgagguc      720

acaagagccu gccuggggua uucguucgac cuuaccgugg uaauggagaa ugaaaaccug      780

uuggaugagu caggaaagaa gauuaagauc agcuuuagug acuccaaaua cgaagaacag      840

gaacucgagc ugcagaauag guuagaggaa aaguucgcug uuauugaggc ccugaaacgg      900

aucuauaacu ggcucgugcu uaaggauacg cugcgaggua aaaaguacau cucugaagcg      960

auggucaaua aguaugaggc acaucacgaa gacuugguga aacuaaagcg ccuguuuaga     1020

acuuaccuca gcaccgagga guacuccugu uucuuuagga aagaaaagca gaaagauaag     1080

uauauuucaa acuacgugaa cuaucggaag ggcgccgagc gcugcaguaa agaaaagcug     1140

uacaaagcua uacacgagcu uuucgacaca gucgccaagg augacguugu guaucaagag     1200

aucguagaga aaauggauca gggggaguuu cugacuaagc agaaugaagu gagcaacgca     1260

uacauuccau aucaguguaa ugaggucgaa augaagagaa ucuugaaaca acagggagag     1320

uucuacccgg agcucaagga aaacgcugac aaaauacugc aguuacugcu auuuaggauu     1380

cccuacuaug ugggcccucu cguugaugag aagcagucua aauucgccug gaugaagcgu     1440

cggaccggua uggaacaagu gaaaaucuac ccauggaauu uugagcaggu cguggacauc     1500

gaccagacag cggaagccuu cauuacccga augacgaacu auugcacuua ccugcccgag     1560

gagaagguac uuccuaagca guccuugcug uauucagaau acgagcuccu gcaagaacuu     1620

aauaaaauca agauuaacgg gaaauuuagc cuggagacaa gaaauaagau aaucgaacag     1680

uuguucaaaa agcagaagaa aguuaccgag aagcaacuca aaaaguggcu ggugagggag     1740

caguauugua cuacaaaagu caccgaaaag auggugaagu uagaugagaa cgcauacuac     1800

auuacgggau uccagaaaga aaaugaguuu gcuaguucuc ugaccuccua uaucgacuuc     1860

acaaagaucc uaggcgagau uacugaacgc aaccggaaaa ugauagagga aaucauuuac     1920

uggcucaccg ucuuugagga aaagagcauc gugaaaagaa agauucagaa gaaauauggc     1980

aaugagauca acgauaagca acuggcccag auacuuaaau ugaauuacuc ggggugggga     2040

agguucucug agaaguuucu gaacgguauc aaaggcuucg accgccaggg gcagaagaaa     2100

acaauuaugg aacuccugcg agaggauucc cgguuacuga acaucaugca aauuaucaau     2160

aacgaagaga ugggauuuug caagauaauu gaagcuaauc agaagaaacu uuauggcaac     2220

gagaauuugc ucgacguugu gcaggagcug caugguucac cagccaucaa gcgugggauu     2280

uggcagaguc uaaaaaucgu agaagagaua aucagcauua ugggcggagu cccccaaaac     2340

aucuucauug aauuugcaag agaggaugac gauagggugc gcacuaauuc ccggguuaag     2400

cagaucgaaa gacuguacaa aaaguucaag gaggagguga acgacuuuaa ugaacacgcc     2460

gcuaacgagc ucaaagaaaa gagguaucag aaucgaauag augaggagaa agucuuccug     2520

uacuuuaugc aaaacggcaa gucucuuuac agcgggguga ccuuggacau ugaucgccug     2580

aaugaauaug agguagacca uaucaucccu cggacauuag ugaaagacga uucacucgaa     2640

aacaaggcgc ugguucuuaa gggagagaau cagagaaaag gugaaggccu gauuccacac     2700

gaguggcaga acaggcagaa ggaggucugg aaaaaguugc ucaguugugg gcugaugggc     2760

aaaaagaagu acgcaaaccu augcaaagaa uccuucggaa augagaagga acaaaaacac     2820

uucaucgccc gucagauugu ggagacccgc cagauaauca aacagguggc uaacauucug     2880

gcccaauauu accagggugu caauguugca gccgugaaag cuagccucgg gucugaacug     2940

cgggagaacu auacuaucga gaagaaggac gauaauggcu uuuucaugga aaacaaauca     3000

uacacgaugc uuaagaguag aaaaauuaau gacuuucauc acgcccagga ugcauauuug     3060

gcgugugcug ugggacugua caucaaggcc gcauucccga aauacgagaa ggaaauagac     3120

uaugagggcu acaggaaaua uuaccagaag aagaaccaac agaaagaugg gguacgaaau     3180

gaaaagcggc aguuuuucag cuaugucaug ucccgcuuuu cugagcagau cguggacaaa     3240

gagacaggag aaauuuuaug ggaugguacc caaaagcucg ccuacaucaa aaagauuuuc     3300

ggcuauagag acugccugau cacuaagaaa cuugaggaaa acaccgggga guuuuacaag     3360

cagacaauaa augcuaaacc cgagaaggga aaagaaaaca auaccaagcu gcuaccuauu     3420

aagaaaggcc ucucgguuga gaaguacggu ggguaucagg gcggagaagc cgcauacagc     3480

ugucugguga gguaucaaca gggccggaaa acuaaguugu cccuggagaa aaucucaguc     3540

aucauugcua agaagauagc cgaaaaagag gugaguuuag aucgauaccu caaggagaaa     3600

cuggggguug aagagaucgu gauucuuaga aagaaaguaa ugaagaacca gcugaucgaa     3660

gacucugaug gaaaucgcuu cuauuugaca gcggccacgg aggucgugaa cgcuaaacag     3720

uuuauucucg acggcaagca ucaacaucug cuaaggacca ucgcaauaau ggaaaagggu     3780

gaguacuaug gguaccgucu ggacgagcuu gaaaacagca uggaugagcu cuacgccuau     3840

uaccggauga aaauccagca gcaguaucca gcuuacgaau ccauugugaa gaaaaucguc     3900

gagucacacg uuuaugaaag ccugcacgug gaggagaaga uuaaagccgu acaagaaaag     3960

ggaaaguucu ugauccagcu guuaagacuc acugaggcaa augcggaaug cauagaccug     4020

aaaacaauuu cugauggcag gcuugccgac cgcaucgguc gaaagaacca cuacaccauc     4080

aaagagggca uuaaguucau cgaucagucc auaacagggc uguaugagag auacaugacu     4140

guguga                                                                4146


<210>  3212
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3212
auggacuacg ugaaggagua ucacaucggc cuggauauug ggaccagcuc cauaggaugg       60

gccgucacag acucucaguu caaacucaug agaaucaagg guaaaacugc uauuggcguu      120

aggcuuuuug aagaggggaa gacggcagcc gaacggcgca ccuuccgaac aacucguaga      180

aggcugaaac ggcgcaagug gagauugcau uacuuagaua agaucuuugc gccccaucug      240

caagaggugg acgaaaacuu ccuaaggcgg cucaaacagu caaauauuca cccugaggau      300

ccagcuaaga accaggccuu uaucggaaaa cugcuuuucc cggaccuguu gaagaaaaau      360

gagcgcggcu aucccacccu cauaaagaug cgagaugaac ugccuguaga gcagagagca      420

cacuacccag ugacaaacau cuauaagcug agggaagcca ugauuaauga ggaccggcaa      480

uuugaucucc gugaggucua cuuagcugug caucacaucg uuaaauaucg cggucacuuc      540

cugaacaaug caagugugga caaguuuaaa gucgggagaa uugauuucga caagagcuuu      600

aacgugcuua augaagccua cgaggaauug cagaacggag agggcuccuu caccaucgaa      660

cccucuaaag uagagaagau aggccagcug cuacucgaua cuaaaaugag gaagcuggac      720

cggcagaagg cuguugccaa acuucuggag gugaaggucg cggacaaaga agagacaaag      780

cgaaauaaac aaauugcaac cgccaugucg aaguuggugc ucggguacaa ggcugauuuu      840

gccacggugg caauggcuaa cggaaaugaa uggaaaaucg accugucaag cgagacgagu      900

gaagaugaga uugagaaguu ccgcgaagag uuauccgacg cccagaacga uauccugacc      960

gaaaucacau cucucuucag ccagauuaug cuuaaugcga uagucccuaa cgguaugucc     1020

aucucagaga guaugaugga cagauauugg acucaugaaa ggcagcuggc cgagguuaaa     1080

gaguacuugg caacccaacc agcuagcgcc cggaaggaau uugaucaggu guauaacaaa     1140

uacauuggcc agguacccaa ggagaagggg uucgaccuag aaaaaggacu gaagaaaauc     1200

cucucuaaga aagagaauug gaaggagaua gaugaacugc uuaaggcugg cgacuuucug     1260

ccuaaacaac gcacauccgc caacggugug auuccacacc agaugcauca gcaggaguug     1320

gauagaauca ucgaaaagca agcaaaauau uacccguggc ucgccaccga gaaucccgcu     1380

acgggggaaa gggaccguca ccaggcgaag uaugagcugg accaguuagu cucauuccgg     1440

auuccuuacu acgugggacc acugguuacu cccgaggugc agaaagcaac aagcggcgcc     1500

aaguuugcuu gggccaaacg aaaggaagau ggcgagauca ccccuuggaa ccuaugggac     1560

aagauugaua gagcagaaag ugccgaggcu uucauaaaaa ggaugacugu caaggacaca     1620

uaucuccuga augaagaugu gcuuccagcc aacucuuugc uguaccaaaa auauaaugua     1680

cucaacgagc ugaauaacgu ucgcgugaau gggcggagac uuuccgucgg aaucaagcag     1740

gacauuuaca ccgagcuguu uaaaaagaag aaaacgguga aggcaggcga ugucgcgagc     1800

uuggugaugg cuaaaaccag ggguguuaac aagcccucgg uggaagggcu cucugacccg     1860

aaaaaguuca auuccaaccu ggccacauau uuagaucuga agucaaucau gggagacaaa     1920

guagaugaca aucgcuacca gauggaucua gagaacauca uugaauggcg aagugucuuu     1980

gaggacggcg aaauauucgc agacaagcuc acugaggugg aguggcugac cgaugaacag     2040

cggagcgccc uuguuaaaaa gcguuauaaa gguuggggga gauuguccaa gaagcugcuc     2100

acaggcaucg uggacgagaa cggacaaagg auuaucgauc ugauguggaa uacugaccag     2160

aacuuuaugc agauugucaa ucagccugug uucaaagaac aaaucgauca guuaaaccag     2220

aaggcuauaa ccaaugacgg caugacacug cgcgagcggg uagagucugu gcuugaugac     2280

gccuacacca gcccccaaaa caaaaaggca aucuggcagg uugucagagu gguggaagau     2340

auugucaaag cuguugggaa ugccccaaag ucaaucagua uugaguuugc gaggaacgaa     2400

ggaaauaaag gugagaucac ucgaucccgc cggacgcagu ugcagaaacu cuucgaagac     2460

caagcccacg agcuggugaa ggauacaagc cuaaccgagg aacuggagaa agcuccugac     2520

cucucugaua gauacuauuu cuacuuuacu cagggcggga aggacaugua uaccggagac     2580

cccauaaacu ucgaugaaau uucaacaaaa uacgacaucg aucauauucu gccacagagu     2640

uuugugaagg acgauagccu ugacaauagg guauuggucu cccgugcaga gaacaauaaa     2700

aagucugauc gcgugcccgc caagcuguau gcugccaaaa ugaagccuua cuggaaccag     2760

uuacucaaac aaggccugau cacccagcgg aaguucgaaa aucuuacuau ggacguugau     2820

cagaacauaa aauauagauc gcuggguuuu gugaagaggc aguuggucga gacacgacaa     2880

gugaucaagc ucacggcaaa cauucugggg agcauguacc aggaggcggg caccgacauc     2940

auugaaacuc gggccggacu aacaaaacag cugcgcgagg aguucgaucu cccaaagguu     3000

agagaaguga augacuacca ccaugcugua gacgccuauc ugaccacauu ugcaggccaa     3060

uaccuuaaca ggcgguaucc gaaauugcga uccuucuuug ucuacgggga auauaugaag     3120

uucaaacacg gaucagaucu gaaguuaaga aauuuuaacu ucuuucauga gcucauggag     3180

ggugacaaga gucagggcaa agugguggau cagcagacug gggaacugau caccacgcgc     3240

gacgaggucg cuaagucuuu cgauaggcuu cugaauauga aauacaugcu aguuagcaag     3300

gaagugcacg accguuccga ucaacucuau ggagccacca uaguaacagc aaaagaguca     3360

ggcaagcuga cuagccccau ugagaucaag aaaaaccggc cuguggacuu guacggugcc     3420

uacaccaaug ggacaucugc uuucaugacu aucauuaagu uuaccggcaa caaaccaaag     3480

uauaaaguca ucggagugcc cacgacaucc gcgguuggcc ugaagagagu ggggaaaccu     3540

ggaagugaaa gcuacaauca ggaguuacac aggauuauaa agucuaaccc aaaggucaaa     3600

aaggauuucg aaaucguggu uccccaugug uccuauucac agcucauugu cgacggcgau     3660

ugcaaauuua cccuggccag ugacacuuac cagcacccgg caacacaacu uguacugagc     3720

aaggagucga uggaaaucau ugcugaugac uucaaauuug ugaaggagaa uccugccacc     3780

gccgacgagc aguugguucg cguguucgau gaaaucguca accagaugaa ucauuauuuu     3840

acuauauucg accagcgauc caacagacaa aaggugacca aagcucggga uaaguuugua     3900

ucucucccaa cagagagcga auacgagggu gcaaagaaga cucagguggg gaaaaccgaa     3960

gucaucacua aucugcuaau gggacugcac gccaacgcgg cucagggcga ccuuaagguu     4020

cucggucugu caacauucgg cuuuuuccag aguuccgggg gauugaaccu gucugaggau     4080

gccaugauug uguaucaaag ccccaccggc uuauucgaga ggcgcaucug ucucaaggac     4140

auuuga                                                                4146


<210>  3213
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3213
augaagaaaa gcuacgccau cggcauugac auagggacca acuccguggg acacugcguc       60

auguauacag agaauuacaa gguucccucu aaaaagauga aaguguucgg uaacacugaa      120

aagagauaua ucaagaaaaa ucugcucggc acgcuucugu uugaugaggg gaacaccgcu      180

gaaaauaggc gguugaagcg cacagcacga cguagauaca cuaggcggcg caacagaauu      240

uuauaucugc aggagaucuu cgccgaagag auuaauaaaa ucgacgauuc auuuuuccaa      300

aggcuagacg auaguuuucu cauaguagag gacaagcagg gaagcaaaca uccuaucuuc      360

ggcacccugc aggaagagaa ggaauaccac aagcaguuuc caacaauuua ucaucuucgg      420

aaacaacugg cggauuccuc ucagaaggcu gacauccgcu ugauuuaccu cgcccuggca      480

cacaucauaa aauaccgagg ucauuuccug uuugaggggg aucucaaguc ggagaacaaa      540

gacgugcagc acuuauucaa ugauuuuguc gaaauguucg acaagaccgu ggagggauca      600

uaucugagcg aaaaccuucc gaauguugcc gauguguugg ucgagaaagu gaguaagucc      660

agaaggcugg aaaacauucu acacuacuuu cccaaugaga agaaaaacgg ccucuucggc      720

aauuuccugg cucuugcacu gggguugcag ccuaacuuua agacuaacuu cgagcucgcc      780

gaagacgcua aaauccaauu uucuaaggag acauaugaag aggaccugga agaguuacug      840

ggaaaaauug gugaugacua cgccgaucuc uucaucgcga ccaagagccu uuaugacggc      900

auccuguugg cagggauucu auccacgacu gauucaacca caaaggcccc acugaguagc      960

ucuaugguaa aucgguacga ggaacaucag aaagaccucg cucugcuuaa ggauuuuaua     1020

cgucagaacc uguccgacuc auauaaagag uucuuuaaug auacuuugaa ggacggauac     1080

gccggcuaca ucgaagguaa aaccacacag gagaacuucu aucgcuuuau uaagaaggca     1140

aucgaaaaga uagaggggag caauuacuuc auugauaaaa ucgacagaga ggacuuucuc     1200

aggaaacaac ggaccuucga uaacggaagu aucccccacc agauucaucu gcaggaaaug     1260

cacgcuauca uucgacgcca agccgaguuu uauccuuucu uaguugaaaa ucaggacaag     1320

auagagaaaa uccugacguu caggauucca uacuaugugg gcccccuagc gagaggcaag     1380

ucugaguuug ccuggcucaa gcggaaaucc gaugaaaaga uccgcccuug gaacuucgac     1440

gagauggucg auaaagaaac uagcgcagag aauuuuauca caagaaugac caacuacgac     1500

caguaucugc cagaucagaa ggugcuuccg aaacacucgu ugcuguacga aaaguucgcu     1560

guguacaaug agcucacuaa agucagguau guuacagagc aagggaaguc uuucuucuuu     1620

gacgccaaca ugaagcagga aauuuucgau ggaguguuua aaguauaccg uaaggugacc     1680

aaagagaagc ugauggacuu ccuugguaaa gaauuugaug aguuccggau agucgaccug     1740

augggcuugg auaaggacaa uaaguccuuu aacgcuucac ucgggacgua ucaugaccug     1800

aaaaagaucg ugaguaaaga uuuacuggac aaucccgaaa acgaggauau ucuagagaac     1860

guugugcuca cccugacacu uuucgaagac cgagagauga ucagaaagag guuggaaaaa     1920

uacaaggaug uccugacuga ggaacagcgc aagaaacucg agcggagaca cuauaccgga     1980

uggggcaggc ugagcgccaa guuaauuaau gguauccgca acaaaacaac ucgaaagacc     2040

auacuggacu accuuaucga ugacgggaca uccaaucgga acuucaugca guugauucau     2100

gaugacaccc ucucuuuugu ggaugagauc cgucuggcac aaggcagcgg agaagccgag     2160

gacuauagaa cugaaguaca gaaucuagcu ggcagcccug cgauuaaaaa ggggauccug     2220

cagagucuca agauaguuga ugagcugauu ggagugaugg guuacaaccc agagcacguc     2280

guggucgaaa uggcaaggga gaaucaguuc acgaaccaag gccgccgguc cagccagcag     2340

agauacaaaa agaucgaaaa ugccauuaaa aaccuugacu cuaauuugaa cucaaagauc     2400

cugaaagagu aucccacaaa uaaccaggcu uuacaaaaug acaggcucuu ucuguacuau     2460

cuucagaacg ggaaggauau guacaccgac gaagaacugg auauagacca guugagucaa     2520

uaugauaucg accauauuau cccucaggcc uucauuaaag augacagccu cgauaacaag     2580

gugcugacua aguccgccaa aaaucgagga aagucugacg auguuccauc gcuagagauc     2640

gugcacaaaa agaaaaacuu uuggaagcag cugcucgaca gccagcugau aucccaacgc     2700

aaguucgaca aucuuaccaa agcagaacgg ggcgguuuga caaacgagga uaaggcuaga     2760

uuuauucaga ggcagcuggu agaaacccgu cagaucacua aacacgucgc ccgcaucuua     2820

gacacacggu ucaauacgaa gcucgaugac gcagggaaca gaauuaggga ucccaaagug     2880

aauaucauua cccugaaguc aagucuuguu ucucaauuuc gaaaggacua ccagcuguau     2940

aaagugcggg agauaaacaa uuaccaucac gcgcaugaug cuuaccuaaa cgccgucgug     3000

gcaacugccc uccugaagaa auauccgcag uuggcuccug aauuuguaua cggcgacuau     3060

cccaaguaca auagcuauaa aucccgcaag ucagccacag agagagugcu guuuuacagc     3120

aacaucauga auuucuucaa gaggguuguc aaaucuucca agaccggaac agugaaaauu     3180

cggccaauca uugaggugaa caaggaaacu ggcgagaucg ucugggauaa aaagagugac     3240

uuucgaaccg uuagaaaggu guuaagcuau ccucagguga auguagucaa aaagacggaa     3300

gugcaaaacc acgggcucga ucgcggaaaa cccaaggguu ucuacaacgc aaaucugucu     3360

ccaaggccca aagagggcuc cguugaaaac cuugugccug cuaagcaguc auuugacacc     3420

aaacguuacg ggggauaugc cggcauaagu aauagcuacg cgguccuggu gaacgguauc     3480

auugagaagg ggaagaaaaa gucgaaagcc gagguuacag aguuccaggg caucuccauu     3540

uuggcucgga aggauuuuga gaaaaaucca aagcaauauc uccugaaccu aggauacaag     3600

gacaucaaau cuauaauuaa gcugccgaaa uauagccuuu ucgaacucga gaauggcuca     3660

agaaggaugc uggcaaguau cuuguccacu aacaauaagc gcggggagau ccacaaagga     3720

aacgaaaugu accugcccga caaguuugug accuuacucu aucaugccau gcgaguaaau     3780

agaacacugg agccuggcca caagaaauac gucgaaacuc aucggcacgu guucgaugag     3840

cuucugaccu acauuuugga auauaacgag aagauagugg gugcuaaagc caaugggaag     3900

aggaucgucg aggcauacuc ugcgcgcaaa gacacggaua accucgagga acuguguaau     3960

agcuuuauua accuacugaa ucuuacagcc cucggauccg cuguugacuu cgaauuucug     4020

ggcaccaaga ucccacguua uagagauuac acucccucau cauuguuaaa ggccacacug     4080

auucaucaga gugugaccgg ucucuaugag acucggaucg accugucuaa acuuggcggg     4140

gauuga                                                                4146


<210>  3214
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3214
augaagaaaa ucauuggccu ggaccucggg accacaagca uaggaugggc cuacgugcac       60

gaggcugaau ccgcacagga gagaucugaa aucgucaggc uuggugcccg gguugugccc      120

cugacuacgg augagcaaga cgaguucaag aaaggcaagu caauuaccac aaacgcggcu      180

cgcuugagua aacgaucauu acguagaaau gccgauaggu uucagcugcg gcgcgacgaa      240

cuagcagggg uaaugaagga ggccggaugg aucgaugaaa cuaccgugcu cgcugagcag      300

ggcccuagau ccacauucga aacccugagg cuucgggcaa aggccgcuuc ugaggagguc      360

acucuggccg aauuugcgcg cauuuugcuc cagaucaacc gaaaaagagg uuauaagucg      420

aauaggaaaa cagacgggga ugaggaagga gcacugauag acggcaugga ugugucaaag      480

caacugaacg ccagcggcca gaccccaggg gaguggguuc ucgcucggau gcaggaagga      540

aaugccguga ucccggcauu cuacugcagu gacuuauccg aggagcugga ucguauuugg      600

cgcaaacagc aggcuucuca ucaagccugg augacggagg aacuucacac ugucuugaga      660

caugcggacg guaggagcgc cauccggcug auggauaagg caggcguggu acccgacucc      720

guuaacguga aggaacgacg cagagucaug uaugagugga gggcugccgc ugugcgggac      780

ugucuaccug ccgggcgcgu ggcagccgcu cucaugcagg ucucaaaaga aauuggcaga      840

gcggauaguu accugggaag gaucggugac cguagcaagg agcuuuauuu ucaccggcag      900

accguugggc aauaccugug gcgauugaua ugcgauaauc caaaccaguc ucucaaaaau      960

cugguguucu auagacagga cuaccaacau gaguuugaaa cauuaugguc cauucagacu     1020

aaguaccacg uagagcugac cgaagaucuc aggaaacgcg ugggacggau cauuuucuuu     1080

cagagacccc uuaagagcaa aaagggccug gucaacaggu gugaguuggu gcacuauagc     1140

guuugcgaaa auaguggcga cgaucuaaca gagaaggugg acgucgggcc uaaagugugu     1200

ccaaagucuu ccccacuguu ccaggaguuu aaaguauggc aaguucucaa caauguggaa     1260

gucauccauc cuagcaccca ggaggcacgc cgacuggaaa uggaggagaa ggaacuucug     1320

gccucggagc uacucuuccg ggaucguaug aaaaagaucg acgugcugaa guuacuguuu     1380

ucuaacugga gagaucuaga ccucaauuac aaagaacugg agggaaacag gacgauggcu     1440

gccuucuauc gcgucuacga acaggugauu gcacagacug gucacggcga cuaugauuuc     1500

gccaagaugc gggcugccaa aguuaaggag cuugcagagg ggguguuugc gggauugggc     1560

uucgaaguag gucugcucac auuugacgcu gagcuggauu ccccagccua cgaacaacag     1620

ccgugcuaua gacuuuggca ucuguuguac ucauacgagg gggacaaaag ugcaaccggc     1680

aacaaguguc uccugaagaa auuaugcagg cugacuggaa ugaaugccga auuggucggc     1740

cucuucaugc agauacgauu uagcggggau uauggauccc ugucuacaaa ggcuaugcgc     1800

cggauccuuc ccuacuugaa agccgguaac accuaugacg uggcauguga guacgcuggc     1860

uucagacaca gcgccagguc acugacgcgu gaggaacucg cgcagaagga guauaaagau     1920

uaccuggacu uacugccucg caauagucuu cggaacccag uuguggaaaa gauuuugaau     1980

caaaugaucc acgucgugaa cgggcucucc agcaccuaug gaaagcccga ugagauaaga     2040

guagaaaugg ccagggagcu gaaaaagucu gcucgagagc gggaagagau gacaucagca     2100

auuaguaaaa cuaccaagga aaaugccgag aucacagcua uccuacagcg cgacuuuggc     2160

cugagauacg ugagcaggaa cgauauucuc cgguacaaac uguauugcga gcuucgaauc     2220

aaggguaaug gguguagaac uuuguacucc aacaccuaua uuaauccuaa cgaacuguuc     2280

ucucgcgagu uugacauaga acauaucauu ccacagucga gguuauucga ugacagcuuu     2340

uccaauaaaa cacucgaguc acguaccgcc aaccuggaaa agggcgaccg gacugcaugg     2400

gauuacguua augagaagua uggagcggag agagccgacg aauacagggc ucaggucgag     2460

gcccuuuauc gcgauggcac gaucaguaaa gcaaagcgag acaaacuguu gaugcccggg     2520

gauaagaucc cggaagacuu cauugauaga gacauacgga acacacaaua caucgcuaaa     2580

aaggcccucg agauuuuuga agauuucgug ccuucugugg ucgcaaccag cggaaacguu     2640

acugccaggc ugcgcgagga cuggggucua guggauguga ugaaggagcu gaauuggucc     2700

aaauacgaau cacagggcau gguacacauc gaguaugacc gugaugggag acccauucgg     2760

aggaucgucg acuggaccaa gcgcaacgac caucgacacc augcuaugga ugcgcucgcc     2820

guggcauuca caaaaagaag cuacauacag uaucugaauc accuuaacgc ucggaaggaa     2880

gccaccucug uugccuacgc uauccaacac aaagagaugg aauuggacgg auccaauagg     2940

uggcgcuuua agugcccauu cggcgauaga agugcauuua ggcgggaggc caaggcgcag     3000

cuggagcgua uuuuagugag caucaaaucu aagaacaaag uccucacucc uuccgugaau     3060

cgcacaaaga aacgaggugg guucaacaag aagguugaau ggacgcccag aggcgcucug     3120

cauaaugaga ccguguaugg aaggauucgg cgcuacgacg uaaaagaacu uagagucggc     3180

ucaaguuuua cugccgaggu gaucgggaag guggcagaua gcagguaucg ggccgcucug     3240

augcaccgac uagcagaguu cggaggcgac cccaaaaagg ccuuuacagg uaaaaacucg     3300

cucgaaaaga auccccugua caccgaugag gcgcauacag cuugcguccc uuccucuguu     3360

aaagugguga gcuucguaga aauagggacu auuagaaagg gagucgaccc aaacuugaau     3420

guggauaagg uuguggacac ccacaucaaa aacauucugu uagcccgccu cucagcauac     3480

ggcggugaug ccaagaaggc uuuuaguaau cuggacgccg acccgaucug gcuuaacaaa     3540

gagaaaggca uagcaaucaa gagggucacg auuuccgaug acucugaagu gcggcccauc     3600

cacguuaagc gugauaugag agggaggcug gugugugacg agcauggacg cccuguccca     3660

agcgauuaug uacaguugcg gaacaaucac cauguggcua uuuuccgaga cgccaacggc     3720

gagcucaaag aaagaguugu gaccuuuuuc gaggcggucg aaagggugcg ccagcugcgg     3780

uccgagggga gagccguaga agugaucgau aagaauuaca aaugcgagga gggauggaca     3840

uuucuauucu caaugaagau aaacgaauau uucgucuuuc ccgacgcagc uagcggcuuc     3900

gauccucgug agauugaccu gacugaucca gccaauuacg cucuuaucag uccccaccuc     3960

uuuaggguuc aaaaacuguc uuccaaggac uauuacuucc ggcaucacuu ggaaaccaca     4020

gugaacgaug ugaagcagcu gcgcgacauc acuuggaaac gaauuagcuc guuacaggac     4080

cucgcaggua ccgucaaggu gagaguuaau cacaucgggc agauuguagc cgugggagag     4140

uauuga                                                                4146


<210>  3215
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3215
auggugaaga aaaaguacgc caucggcauu gacauaggga ccaacagcgu cggauggucc       60

aucguuacag augacuauaa agugcccucu aagaaaauga agguauucgg uaauacugag      120

aagagauaca uuaaaaagaa ccugcucggc acgcuucugu uugaugaagg gaauaccgcu      180

gagaacaggc gguugaaacg cacagcacga cguagauaua cuaggcggcg caauagaauc      240

uuauaccugc aggaaauuuu cgccgaggaa aucaacaaga uagacgauuc auuuuuccaa      300

aggcuagacg auaguuuucu caucguggag gacaaacagg gaagcaagca cccuauuuuc      360

ggcacccugc aggaggaaaa ggaguaucau aaacaguuuc caacaaucua ccaccuucgg      420

aagcaacugg cggauuccuc ucagaaagcu gacauucgcu ugaucuaucu cgcccuggca      480

cauauaauua aguaccgagg ucacuuccug uuugaagggg aucucaaauc ggagaauaag      540

gacguccagc auuuauucaa cgauuuugug gagauguucg acaaaaccgu ugaaggauca      600

uaccugagcg agaaucuucc gaacguggcc gacgucuugg uggaaaaggu aaguaagucc      660

agaaggcugg agaauauccu acacuauuuu cccaacgaga aaaagaaugg ccucuucggc      720

aacuuccugg cucuugcacu gggguugcag ccuaauuuua aaacuaacuu cgagcucgcc      780

gaggaugcua agauucaauu uucuaaagaa acauacgagg aagaccugga ggaauuacug      840

ggaaagaucg gugaugacua ugccgaucuc uucaucgcga ccaagagccu uuacgacggc      900

auucuguugg cagggauacu auccacgacu gauucaacca caaaagcccc acugaguagc      960

ucuaugguua accgguauga ggagcaccag aaggaccucg cucugcuuaa aaauuuuauc     1020

caucagaacc uguccgauuc auacaaggaa guguucaaug acaaauugaa ggauggauau     1080

gccggcuaca uugaggguaa gacuacccag gaaaacuuuu accguuucau caaaaaggca     1140

auagagaaaa uugaagggag caauuauuuu aucgacaaga ucgaccgcga ggauuuccuc     1200

agaaaacaaa ggacauuuga caacggaagu auuccccacc agauccaucu gcaggagaug     1260

cacgcuauua uacggcgaca agccgaauuu uacccuuucu uagucgagaa ucaggauaag     1320

aucgaaaaga uucugaccuu ccgcauccca uauuacgugg gcccccuagc gagaggcaaa     1380

ucugaguucg ccuggcucaa caggaagucc gacgagaaaa uccggccuug gaauuuugau     1440

gaaauggugg acaaggagac gagcgcagaa aacuucauua cucgcaugac aaauuaugau     1500

caguaccugc cagaccagaa aguccuuccg aagcacucgu ugcuguauga gaaauuugcu     1560

guuuacaacg aacucaccaa ggugagauac guaacugagc aagggaaguc uuucuuuuuc     1620

gaugccaaua ugaaacagga gauauuugac aggacauuca agguguaucg uaaagucacc     1680

aaggaaaaac ugauggauuu ucuuuccaag gaguucgacg aauuucggau cguggaucug     1740

uugggacucg acaaggacaa caaaucauuc aaugcuaguc uggguacgua ccaugauuua     1800

aagaaaauug uuagcgagga cuuucuggau aacuccgaaa acgaggacau ccuagagaau     1860

gugguccuca cccugacacu uuucgaagau cgagagauga uuagaaagag guuggaaaaa     1920

uauaaggacg ugcugacuga ggaacagcgc aagaaacucg agcggagaca cuacaccggc     1980

ugggggaggc ugucugccaa guuaaucaac ggaauacgcg auaaaguaac acgaaagacu     2040

auccuggacu aucuuauuga ugacggcacc agcaaucgga acuucaugca guugaucaau     2100

gaugacacac ucucauuugu ugaugagauu cgucuggcac aagguagugg gaaagccgaa     2160

gacuacagag cugaggugca gaaccuagcg ggcucccccg caaucaagaa gggaauacug     2220

cagagccuca aaauugucga cgaacugauc gaggugaugg gcuaugaucc ugagcauauu     2280

gucguggaaa uggccaggga gaaucaguuc accaaccaag ggcgcagaaa uucucagcag     2340

cgguacaaga aaaucgaaaa cgcuauaaag aaucuugacu caaacuugaa uaguaaaauc     2400

cugaaggagu acccaacuaa caaucaggcc uuacaaaacg auaggcucuu ucuguauuac     2460

cuucagaacg gaaaagacau guauacggau gaagaacugg acauugauca guugagccaa     2520

uacgacaucg aucacauuau cccccaggca uucauaaagg acgauucccu cgacaauaag     2580

guucugacaa aaucugccaa gaaccgaggu aaaucggacg augugccuag ccuagagauu     2640

guauauaaga aaaagaauuu uuggcagcag cugcucgacu cccaacugau cucacagcgc     2700

aaguucgaua accuuaccaa agcugaacgg ggcggguuga cuaaugagga caaggccaga     2760

uuuauccaga ggcagcuggu cgaaacccgu caaauuacaa aacauguggc acgcaucuua     2820

gauacccggu ucaacacuaa gcucgacgag gcgggaaaua gaauuaggga uccaaaaguu     2880

aacauaauca uucugaagag uaaucuugug ucucaguuuc gaaaggacua ccagcuguau     2940

aaaguccggg aaaucaacaa uuaccaccac gcucaugaug ccuaccuaaa cgcaguggua     3000

gccacagcuc uccugaagaa auauccccag uuggccccgg aguucgugua cggcgaguau     3060

ccuacguaca auagcuauaa cucccgcaag ucagcaaccg aaaaaguucu guuuuacagc     3120

aauauuauga acuucuucaa gagaaucguc gugcacucua agacugguga ggugaggaua     3180

cggcccguca ucgaaguuaa caaagagaca ggggaaauug ugugggacaa gaaauccaau     3240

uuucgaaccg ugagaaaggu auuaaguuau ccacaaguca acgugguuaa aaagacagag     3300

gugcagaauc auggccucga ucgcggaaag ccuaaaggcu ucuacaacgc uaaucugagc     3360

cccaggccaa aggagggguc ugucgaaaac cuugugcccg ccaaacaguc cuuugacacu     3420

aagcguuacg gagguuaugc gggcaucuca aauaguuacg ccguucuggu gaacgggauu     3480

aucgagaaag gaaagaaaaa gagcaaggcu gaaguaaccg aguuccaagg cauaucgauu     3540

uuggcacgga aagauuuuga caagaauccu aaacaguauc uccugaaccu agguuacaag     3600

gacaucaaau ccaucauuaa gcugccaaag uauucucuuu ucgagcucga aaaugggagc     3660

agaaggaugc uggccucaau auugaguacg aacaauaaac gcggcgagau ccacaaggga     3720

aacgaaaugu accugccgga uaaauuuguc accuuacucu aucacgcuau gcgagugaau     3780

agaacacugg agcccggcca uaagaaauac guggaagccc accggcaugu cuucgacgag     3840

cuucugacuu acauuuugga guauaacgaa aagaucguug gggcaaaggc gaauggaaaa     3900

aggauugugg aggccuacuc cgcucgcaag gauaccgaca accucgagga acugugcaac     3960

ucuuuuauca aucuacugaa ccuuacagcc cucggcagcg caguagauuu cgaauuucug     4020

gguacuaaaa uaccucguua uagagacuac accccauccu cauuguuaaa ggcuacgcug     4080

auccaccaga gcgugacagg gcucuaugag acccggauug aucugaguaa acuuggaggc     4140

gacuga                                                                4146


<210>  3216
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3216
auggugaaga aaaaguacgc caucggcauu gacauaggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa aguacccucu aagaaaauga agguguucgg uaauacugag      120

aagagauaca ucaaaaagaa ccugcucggc acgcuucugu uugaugaagg gaauaccgcu      180

gagaacaggc gguugaaacg cacagcacga cguagauaua cuaggcggcg caauagaauu      240

uuauaccugc aggaaaucuu cgccgaggaa auuaacaaga ucgacgauuc auuuuuccaa      300

aggcuagacg auaguuuucu cauagucgag gacaaacagg gaagcaagca cccuaucuuc      360

ggcacccugc aggaggaaaa ggaguaucau aaacaguuuc caacaauuua ccaccuucgg      420

aagcaacugg cggauuccuc ucagaaagcu gacauccgcu ugauuuaucu cgcccuggca      480

cauaucauaa aguaccgagg ucacuuccug uuugaagggg aucucaaauc ggacaauaag      540

gaugugcagc auuuauucaa cgaguuuguu gagauguucg acaaaaccgu ggaaggauca      600

uaccugagcg agaauauucc ggacgucgcc gauauccuug uggaaaaggu aaguaagucc      660

agaagguugg agaacguucu gcaguauuuu cccaaugaga aaaagaacgg ccuacucggc      720

aauuuccugu cucuugcucu gggguugcaa ccuaacuuca aaacuaauuu ugaacucgca      780

gaggacgcca agauucaguu cagcaaagaa acauacgagg aagaucugga ggaauuacug      840

ggaaagaucg gugacgauua ugcugaccuc uuuaucgcca ccaagucccu uuacgauggc      900

auucuguugg cggggauacu aucaacgacu gacaguacca caaaagcacc acugucuagc      960

uccaugguga accgguauga ggagcaccag aaggaucucg cccugcuuaa agacuucauc     1020

cgucagaacc ugucagauag cuacaaggaa gucuuuaaug acgcuuugaa agacggauau     1080

gccggcuaca uugaggguaa gacuacccaa gaagauuucu acaaguuugu gaaaaagaca     1140

aucgagaaaa uagaagggag ugacuauuuc auugauaaga ucgaccgcga ggauuuucuc     1200

agaaaacaga ggaccuucga caacggaucu aucccccacc agauucaucu gaaggagaug     1260

caagcaauca uuauacggca ggcuaaguuu uacccuuucu uagaagagaa ucaggauaaa     1320

aucgaaaaga uucugacguu ucgaauccca uauuacgugg gcccccuagc ccgcggcaac     1380

uccgaguucg cguggcucaa uagaaaaagc gacgagaaga ucaggccuug gaacuucgau     1440

gaaauugucg acaaagagac uucggccgaa aauuuuauaa cacggaugac caacuaugau     1500

caguaccugc cagaccaaaa gguucuuccg aaacacucuu ugcuguauga gaaguucgca     1560

guguacaaug aacucacuaa gguacgcuac gugacagagc aggggaaauc cuuuuucuuu     1620

gaugcuaaca ugaagcagga gaucuucgac ggagucuuua aaguguauag aaagguuacc     1680

aaagaaaagc ugauggacuu ccuugguaag gaguuugaug aguucaggau uguggaccug     1740

uugggccucg auaaagacaa uaagucauuu aacgccaguc uggggacgua ccaugaucug     1800

aaaaagaucg ucagcaaaga cuuacuagau aaucccgaaa acgaagacau ucucgagaau     1860

gugguacuga cccuuacauu guucgaggau cgugaaauga uccggaagcg acuggagaag     1920

uauaaagaag uucucacuga ggaacagaga aagaaacugg agaggcgcca cuacaccgga     1980

uggggccggu uauccgcuaa gcugauaaac gguaucagag acaaagugac aaggaagacu     2040

auucuugauu auuugaucga cgaugggacc ucuaaccgca auuuuaugca acucauuaac     2100

gacgacacac ugagcuucgu cgaugagauc cgacuagccc agggcucagg agaagcagag     2160

gacuaccggg ccgaagugca gaaucuggcu ggcaguccug cgauaaagaa agggauucuc     2220

cagucccuga agaucgucga ugagcuuauu gaggugaugg gauaugaacc agagcacauc     2280

guuguggaaa uggcacguga gaaccaauuc accaaucagg guagaaggaa cagccagcag     2340

cgcuacaaaa agauagaaaa ugccaucaaa aacuuggacu cuaaucugaa cucaaagauu     2400

uuaaaagagu accccacuaa uaaccaagcu cuccagaaug aucggcuguu ucuuuauuac     2460

cugcagaacg gcaaggacau guauacggau gaggaauugg acaucgauca acucagucag     2520

uacgacauug aucauaucau accucaggcc uucauuaagg acgacagccu ggauaauaaa     2580

guacuaacaa aguccgccaa aaacagaggg aagucugacg augucccauc gcuggagauc     2640

guguauaaaa agaaguccuu uuggaaacag cuccuggaca gccaacuuau cucacagagg     2700

aaguucgaua acuugaccaa agcagaacga ggaggccuga cuaaugagga caaggcucgc     2760

uuuauucagc ggcaguuagu ugaaaccaga caaaucacaa aacacguggc caggaugcuc     2820

gauacccguu ucaacacuaa gcuggacgag gcagguaauc gcauucggga ucccaagguc     2880

aacauaauca cacuuaaaag uaaucuggug ucucaguuua gaaaggacua ccagcuauau     2940

aaaguaaggg agauuaacaa uuaccaucac gcgcaugaug cuuaccucaa cgccgugguu     3000

gcaacggccc uguugaagaa auauccgcag cuggcuccug aguucgucua cggggaauau     3060

cccaccuaca auagcuauaa cucccgaaag ucagccacug aaaagguguu auuuuacagc     3120

aauaucauga acuucuuuaa acggauugug guccacucua agacaggcga gguucgcauc     3180

agaccaguga uagaagugaa uaaagagacc ggagagaucg uaugggacaa gaaauccgau     3240

uucaggacag uccggaaggu gcucaguuau ccucaaguua acguggucaa gaaaacugaa     3300

gugcagaauc acggccugga ccgagggaag cauaaaggau ucuacaacgc aaaccuuagc     3360

cccagaccaa aggaggguuc uguugaaaau cuggugcccg cuaaacaguc cuuugacacc     3420

aagcgcuacg gcggguaugc cggaauuuca aacaguuacg cgguauuggu caauggcauc     3480

auugagaaag guaagaagaa aagcaaggcc gaggugacgg aguuccaagg gaucucgaua     3540

cucgcuagga aagauuuuga caagaacccu aaacaguauc ugcuaaaucu gggcuacaag     3600

gauauuaagu ccaucaucaa acuuccaaag uauucucucu ucgaacugga aaacggaagc     3660

cgucggaugu uggcaucaau ucugaguacc aauaacaaaa gaggcgagau acacaagggg     3720

aaugaaaugu acuuaccgga caaauuugug acacuccugu aucaugccau gagggucaac     3780

cgcacucuug agcccggaca caagaaguac guugagaccc accgacaugu guucgaugaa     3840

cuguugaucu acauucucga guauaaugag aaaaucguag gcgcuaaggc caacgguaaa     3900

agaauugugg aagcauacuc cgcgcggaag gacacagaua aucuggagga acuaugcaac     3960

ucuuuuauca aucugcuuaa ccucacugcc cuggggagcg cugucgacuu cgaguuuuug     4020

ggaaccaaaa uaccuaggua ucgcgauuac acgccauccu cauuacugaa ggccacacuc     4080

auccaccaga gcgugaccgg ccuguaugaa acucguauug accuuaguaa gcuggguggc     4140

gauuga                                                                4146


<210>  3217
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3217
auggugaaga aaaaguacgc caucggcauu gacauaggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa aguacccucu aagaaaauga agguguucgg uaauacugag      120

aagagauaca ucaaaaagaa ccugcucggc acgcuucugu uugaugaagg gaauaccgcu      180

gagaacaggc gguugaaacg cacagcacga cguagauaua cuaggcggcg caauagaauu      240

uuauaccugc aggaaaucuu cgccgaggaa auuaacaaga ucgacgauuc auuuuuccaa      300

aggcuagacg auaguuuucu cauagucgag gacaaacagg gaagcaagca cccuaucuuc      360

ggcacccugc aggaggaaaa ggaguaucau aaacaguuuc caacaauuua ccaccuucgg      420

aagcaacugg cggauuccuc ucagaaagcu gacauccgcu ugauuuaucu cgcccuggca      480

cauaucauaa aguaccgagg ucacuuccug uuugaagggg aucucaaauc ggagaauaag      540

gacgugcagc auuuauucaa cgauuuuguu gagauguucg acaaaaccgu ggaaggauca      600

uaccugagcg agaaucuucc gaacgucgcc gacguguugg uagaaaaggu uaguaagucc      660

agacaccugg agaauauucu acacuauuuu cccaacgaga aaaagaaugg ccucuucggc      720

aacuuccugg cucuugcacu gggguugcag ccuaauuuua aaacuaacuu cgaacucgcc      780

gaggaugcua agauccaauu uucuaaagaa acauacgagg aagaccugga ggaauuacug      840

ggaaagauug gugaugacua ugccgaucuc uucaucgcga ccaagagccu uuacgacggc      900

auccuguugg cagggauucu auccacgacu aacucaacca caaaagcccc acugaguagc      960

ucuaugguga auagguauga ggagcaucag aaggaucucg cucugcuuaa aaacuuuaua     1020

caccagaauc uguccgacuc auacaaggaa gucuucaacg auaaauugaa ggacggauau     1080

gccggcuaca ucgaggguaa gacuacccag gaaaauuuuu accgguucau uaaaaaggca     1140

aucgagaaaa uagaugggag caacuauuuu auugaaaaga ucgagcguga ggacuuccuc     1200

cgcaaacaaa gaacauuuga caauggaagu aucccccauc agauucacuu acaggaaaug     1260

caagcuauca uuaggcggca ggccaaguuc uacccuuuuc uggaggaaaa ccaggauaag     1320

auagagaaaa uccugaccuu ccgcauucca uauuacgugg gcccccuagc gcgaggcaag     1380

ucugaguucg ccuggcucaa uagaaaaucc gacgaaaaga ucaggccuug gaacuuugau     1440

gagauggugg acaaagaaac gagcgcaaag aauuucauca cucggaugac aaacuaugau     1500

caguaccugc cagaccaaaa aguccuuccg aagcacucgu ugcuguauga gaaguuugcu     1560

guuuacaaug aacucaccaa agugcgcuac guaacugagc aggggaaguc uuucuuuuuc     1620

gaugccaaca ugaaacagga gauuuuugac ggaguguuca aggucuauag aaaagugaca     1680

aaggaaaagc ugauggauuu ucuugguaaa gaguucgacg aauuuaggau aguugaucug     1740

uugggccucg acaaggagaa uaaauccuuc aacgcuucac uggggaccua ccaugauuua     1800

aagaaaaucg ugaguaagga cuuucuggac aacagcgaaa augaggauau ucuagagaac     1860

gucgugcuca cgcugacccu uuucgaagac cgugagauga uccggaagcg auuggaaaaa     1920

uauaaggaua uucugacaga ggaacagaga aagaaacucg agaggcgcca cuacacugga     1980

uggggccggc uguccgccaa auuaaucaau gguauaagag acaagguaac caggaagaca     2040

auccuggauu aucuuauuga cgaugggacu ucuaacugca auuucaugca auugaucaac     2100

gacgauaccc ucagcuuugu ugacgagauu cgccuggcac agggcucagg agaagccgag     2160

gauuaccgag cugaagugca gaaucuagcg ggcagucccg caaucaaaaa ggggauacug     2220

cagucccuca aaauugucga cgagcugauc gaggugaugg gauaugaucc ugaacauauu     2280

gucguggaga uggcccggga aaaccaauuc acaaaucagg gucguagaaa cagccagcag     2340

agguacaaga aaaucgagaa ugcuauaaag aaccuugacu cuaauuugaa cucaaaaauc     2400

cugaaggaau acccaaccaa uaaccaagcc uuacagaacg accgccucuu ucuguauuac     2460

cuucagaaug gcaaggauau guauacugac gaggagcugg auauugacca auugagucag     2520

uacgauaucg accacauuau cccccaggca uucauaaaag augacagccu cgauaacaag     2580

guucugacga aauccgccaa gaaucggggg aaaucugacg augugccuuc gcuagaaauu     2640

guauauaaga agaaauccuu uuggaagcag cugcucgaca gccaacugau cucacagaga     2700

aaauucgaca accuuacaaa ggcugagagg ggaggcuuga ccaaugaaga uaaagcccga     2760

uuuauccagc gccagcuggu cgagacucgg caaauuacca agcauguggc aagaauguua     2820

gacacaaggu ucaacaccaa gcucgaugaa gcggguaauc guauccgcga cccaaaaguu     2880

aacauuauaa cucugaagag uaaucuugug ucucaguuuc ggaaagauua ccagcuguau     2940

aaggucagag agaucaacaa uuaccaccac gcucaugacg ccuaccuaaa cgcaguggua     3000

gccacagcuc uccugaaaaa guauccccag uuggccccgg aguucgugua cggggaauau     3060

ccuacguaca auagcuauaa cuccaggaag ucagcaaccg agaaaguucu guuuuacagc     3120

aauauuauga acuucuucaa gcgaaucguc gugcacucua aaacuggcga agugcggauu     3180

cgccccguca ucgagguuaa caaggaaaca ggagagauag ugugggauaa aaaguccgac     3240

uuuagaaccg ugaggaaggu auuaaguuau ccacaaguca augugguuaa aaagacagag     3300

gugcagaacc auggccucga ucgggggaaa ccuaagggau ucuacaaugc uaaccugagc     3360

ccccgaccaa aagaagguuc ugucgagaau cuugugcccg ccaagcaguc cuuugacacu     3420

aaaagauacg gcggguaugc gggaaucuca aacaguuacg ccguucuggu gaauggcauu     3480

aucgaaaagg guaagaaaaa gagcaaagcu gagguaaccg aguuccaagg gauuucgauc     3540

uuggcacgca aggauuuuga caaaaacccu aagcaguauc uccugaaucu aggcuacaag     3600

gauauaaaau ccauuaucaa gcugccaaaa uauucucuuu ucgaacucga gaacggaagc     3660

aggcguaugc uggccucaau cuugaguacg aauaacaagc ggggcgaaau ucacaaaggg     3720

aaugagaugu accugccgga caaguuuguc accuuacucu aucacgcuau gagagugaac     3780

aggacacugg aacccggaca uaagaaauac guggagacuc accgccaugu cuucgacgag     3840

cuucugauau acaucuugga auauaaugag aagauuguug gcgccaaagc aaacgguaag     3900

cgaaucgugg aagcguacuc cgccagaaaa gauaccgaca aucucgagga acuguguaac     3960

ucuuuuauua accuacugaa ucuuacagcu cucgggagcg ccguagauuu cgaguuucug     4020

ggaacuaaga ucccucggua uagggacuac accccauccu cauuguuaaa ggcaacgcug     4080

auacaccaga gcgugacagg ccucuaugag acccgcaucg aucugaguaa acuugguggc     4140

gacuga                                                                4146


<210>  3218
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3218
augaagaaaa ucgugaacua caaucugggc cucgacauug ggaccagcuc cauaggauau       60

gccgcuaucg augaccaggg uagacccguc aggcaccaaa acaagacagu uaugggcgug      120

cggcuuuuca aagaggggaa gacugcagaa ucuacgcgca uuuuucgagg aacccguaga      180

aggcugucac aucggcacug gcgcuugggc uacuuagaua gaaucuucga caaugagauu      240

gccaaaguag augaaaacuu ucugccuagg cuaaaggcga guaagcgggc ucgcagcgac      300

gagaaacgau ccuucagagg uucucuccug uuuccagaaa caggggauga cgccuauuac      360

cagaaguauc cgacuaucua ccaucuuagg uaugcacuga uaacccagaa aaagcaguuc      420

gauauccggu ugauuuaccu cgcccugcac cauaugauca aauaccgugg aaauuuucug      480

gacacaaccc ccgugucggc uuucaacauu cacgauaucg accucacuga ucaauuugca      540

ucauuaaaug acugcuauca gcugcuugac auggauuucg aguuucaguu cgacuugaac      600

aauauagagc agguccaaaa gcugcuacuc gaugaaacac ugagcaagac caaacgccag      660

gccgcuguga guaagguucu ugugaugucc acgggcaaca aagccuuuga cgaucagcag      720

aauggcauug cgucugaguu caucaaggca cugcucgggg gaccuuucga cuugagcaaa      780

auucugaacu uauccgucgc cgaugugaag ucauggaaac ugcacaugac ugacaaggau      840

guagacgaua cccucaauag ucuuguugag gaacuggacg agaaccauaa gcaaaucgug      900

aauuugcuac agaugaucua cagccagguc augaugcugg gucuccugaa cggccaacag      960

ucuauuuccg cuucaaugau agaaagauau gacgaucacg ccaaacauug ggcagcucuu     1020

aagaaacugu ugccagcccu cggggagcag gaacagagca caaucauuca cgcguacacu     1080

gccuauaccg gacacaguag gacaaagcaa cugucucggg cagaguuuua caccaaagug     1140

aacuccaucu uaaagaagca ugaaaauaua gcugaggcca cgggccugau ugagcuaauc     1200

gaccgaaacg aauuuaugcc caaacagcgc gaugcuguga auggugucau cccucaccag     1260

cuccaucaga gagagcugca acuuauuauc cagaaccagc agaaguauua cccauuuuug     1320

gccgcaaaag ccuaccccga cgcugaugaa acuaugauug agcaacugcu cagcuucagg     1380

guuccuuauu acgcggggcc acugguggac cgggauuuua uaaaugaauc gucugaccag     1440

gagcaggcaa ugcagaacac aaaguucucc uggcuuaaac gcagagaggc cggagaaauc     1500

accccgugga auuuugagca aaagguagau gugcaggaaa cugcuaacag guucauuaag     1560

cgucugacaa ccaaagacac guauuugaug accgaggaug uccuccccaa ggaaucacug     1620

aucuaccagc gguuugaggu guuaaaugag cugaacgcca uccgaauuaa ugacagaagg     1680

cuaacagcag aaaacaaaca acucgccuuc cagcagcugu uuauggcugg caauacuaag     1740

aacguuagua ucaaaaaguu ccgcaauuuu guguucgaua gccuuaaguu cccugagaaa     1800

gucaagauaa ccggcuuugc cgggcagcac aacuucuugg caaaacuguc cacauaucau     1860

gacuuuaaga aaauuuucgg agaacgggug gaccacacug aucuccaacc agacuuugag     1920

agaaucguag agugguguac cauuuucgaa gauaggcaca ucuaccgcga gaagcugaaa     1980

gaaauacagu gguuaucuca gagcgagauc gaccagcugg uucuuauucg aaaccauggu     2040

uggggccggu ugucaaagcg ucuccugaca gggaucagag auagugacgg aaggaccgug     2100

auggaucaac uaugguuugg cacugacaau uucaugcgca uuaucuccca gagcgauuuu     2160

gcggcugcca uagcaaacga aaaucggacg cagcugaacc agcacggucu cgaggacguc     2220

cuggauucug ccuucauguc accccaaaau aagaaagcua uuagacaggu gcuuaagguc     2280

guggacgaua ucgagcagac auuggggugc ccucccaaaa agguuaugau ugaauuuacc     2340

aggaguccag acaaaaagaa gcaacgaacu gugacccgcc uggagaaagu acagcaggcc     2400

uauaaggcag ucgcugacca guuagccggc caaagcaucg uguccgaacu cgaucaguac     2460

gcgaacacac auucuaaacu gucggacacc uacuaucuuu acuucaugca gcugggacgg     2520

gauuuguaua gcggcaagcc gauagacauc gauuccccua ugcucgacga ugaccacauu     2580

cugcccagag ccuuuaugaa agauaauuca cuagacaaca gggcucugac ugagaagcca     2640

aucaauaaca caaaggcagc caaauucccu aguaagguuu ucgggcagca aaaagaagug     2700

acggauuuuu ggaagcuccu gcgucgcugu ggacuuaugu cuaauauuaa agagaagcac     2760

uuguaccugg accccgacaa cgucgauaag uggacccgga gagguuucau ccauaggcag     2820

uuaguggaga cuagccaggu aauaaaaauu guggcuaaua uccucaacga ccgauaucag     2880

caaucccaga cagaaguucu ggagguccca gcccggcuug gcaccgaacu gcgcgagugc     2940

uuuggguggu acaaggugag agaagugaau gauuaucacc augucuucga cgcauaccua     3000

gcggccuuua ucggaaacua ccucuauagg cgguacccca aacugcagcc uuauuucguu     3060

uacggcaacu uugcugaguu gaauaaggcc cagcugaaaa acauuaaguc auucaauuuc     3120

uuauauaaca ucacagauaa uaaacgagug gugaaggaca uugagacugg ucaaacccuc     3180

ugggaugacc agauaagaca gcaaauccag cacauuuacg aauauaagua caugcugacg     3240

agccaugagg cauucaccca gcacaaagaa cuuuacgggc agacaaucua uccacgcagg     3300

gaguuuaagc cgggcacucu gaaagcuauu aagcaagauc agccccagga guuguacgga     3360

ggcuauaaaa accucaccgc cgcauacaug uguaucguaa agauacaggc ccguaagcgg     3420

agagcugcgg aauauagggu cgugcgcguu gccacagagu cucuguccgc acuacaacag     3480

gcuaaacgac ugggggugca ggccuacgaa agaugccuuc ggcaggagcu cgucaggaau     3540

aacgaaaucc ugcgcacuaa ugccgacccu aacagugcuc cacgugaguu cgagguguug     3600

cuggauagag uuuuauguca ccaaaugauu aucagcgacg gacagaagua ucuccugggu     3660

ucucaugcau acaaaauuaa uugcaugcag cuugugcugu ccaacaauuc aaugaagauc     3720

uugaguacca gcauaguaaa acucaagcaa augacgaacg aaccccuggu ccagguguac     3780

cgggagauuc uagauguggu caauaaguau uucacacugu acgacguuaa cucguuuagg     3840

gaaaaccuua aucaguucuu ugacaaauuc cgcacccucc cuaucacuaa cgaguaugaa     3900

uccucuaaga gcggcaaaaa gcugauccac cgagggcagu uggagacauu aacccugauu     3960

cucagaggac ugcaugccaa uucagauaaa gugcaauacg gcgaccagag uacucuucug     4020

gguggguuuc agaccaagga uggcauaaag uuguccaaag acucuguaau ccaguauaca     4080

agcccagcgg gacuaaacgu gcggacgguc aagcugaggg aucucgagca aaaagaagcu     4140

cagaaguga                                                             4149


<210>  3219
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3219
augguggacg agaagaucuu cuggggccug gauauuggga ccgacagcgu cggaugggcc       60

guuacaaacu ccgaauacaa acucaagaaa uauaagaaua accuuaugug gggugugcac      120

cuguuugaug aggcuaaaca gucugcagaa agaaggucau uccggacugc ccgccgacgu      180

uuggacagaa ggaagcaacg gauaaucuua cugcaggagu uuuucguacg cgcggugugc      240

gaaaaggaug agaacuuuuu cagaaggcua aaagagagug cucuccugcc cgaagacgcc      300

gagcaucgga cgaauaauau cuuuuucgau gacccugauu acaccgacaa ggauuauuuu      360

gaagaguacc caacaauuca ccaucuuauu ugugagcuga uggaaagcaa agagccgcac      420

gacguccgcu ugguguaucu cgcaugcguu uaccugcugg cccaucgagg ccacuuccuc      480

uuacccgugu ccgaagauga caucucuaag gucacugagu uugaaccucu guaugagucg      540

uucuacaaag cucuugagga aaaguuggac gaugagccac ccuuugacag aucagcagau      600

gacuucgccg aaauacuuaa gagccacaaa accgugagug cuaagaacaa agauuucgac      660

aagcuacucu uugggggaaa aguaaagaca uacgauaaug agaacaucuc cuauucugcc      720

cugauuaaac uucugagcgg cgguaccgaa aaguugucca aguucuuugc gaaugaggag      780

uacacugacc ucgaaaaaga uucaguuugu gugaggaacg cagacuucgg ggauacacug      840

gagauguuag aaggacagau cgacgagcug gacuuugccc uccuuaagag ugucaaaagc      900

cuguaugauu ggucuuugcu aguggacauu cuggaaggca agucccucau cucagaggcu      960

aaaaaggaua aguacgacga gcauggcuau gaucuggacg cccuuaaaua ccuguuccgg     1020

gaauauuuga ccaaggauga cuacaaugag auguuuaaag aagugagcgg gaagcagaac     1080

uacgcaaguu augucuacaa ugcuucuucc gauaaaacgc gugacagcaa guauaagaaa     1140

ugcucgcaaa aggauuucug uaaauuuacu aagaaauucc ucucuaagau aaagccuaac     1200

gagaaagaca agccaugccu ggauaaauua cuggagaagu gugaacaaaa uucccuaugc     1260

cccaaacagg uuaccacaga caaccgcgug auuccuuacc agcucuauua cguagagcug     1320

aagaaaaucc uugaaaacgc cuguggauau uugccauuuc ugaaugagag agacgaauac     1380

gguacugugg cggauaagau ucucucaauc augaaguuca gggucccgua cuaugugggc     1440

ccccugguug acaguaaaaa gagcccuaac gccuggcuug ugcggaaacu ggaugggaag     1500

aucaccccau ggaauuuuac agacaugguc aacgaggaug aguccgaaaa ugcauucauu     1560

cgacgcauga ccugcaaaug uacguacgug gcuggacagg acguauugcc caaguauucu     1620

cuccuguaca gcaaguucuc aguuuuaaac gagauaaaua acaucaaacu gaauggcgaa     1680

ccuauuagug ugcagacuaa gcaggagauc uauacagaac uauuugagag aaacaaaucc     1740

agggucagca agaaaaagau acgggauugc cucauuucuc acgguuacgc cgcugacuca     1800

gaugagguga ccgggaucga cgauaucgcc aagagugcac ugcgcagcua ucaugacuuc     1860

aaaaagaugc uuuccaaugg aauuuugacu gaacagcaag ucgaggaaau cauugagcac     1920

auaacaguga ccacggauaa caucagacug aaaaaguggc ucaaaaccca guuuccaaug     1980

cuggccgacg aagacguuaa guacauuaca aaguuaaaau acaaggauua uggcaggcug     2040

ucucguugcu uccuugagga cguguugccc guagauacua aaaccggcga ggcugaaucg     2100

gacaagaaua ucaucacaau gcucugggag acuaacgaaa auauuaugca gcugcuaagc     2160

uccaaauacc gguauucaga gaacauagag cacaugaacc gacaauacua ugcgcugccg     2220

gaaaaucaua agaguauguc ugagagacuc aaagauaugu acgucccuac cgcagugagg     2280

cgcgccguua cacggaccuu ugacaucgug aaggaacuga agaaaauuca ggggagagau     2340

cccgacaaga ucuucauuga gauggcuagg ggaacuggug aaacgccaaa aggcaagcgc     2400

acaaacagcc gaaaagauca gauccuugag cacuggcaug gguuggacaa uaaggauaua     2460

aacgaccuga agaaauccgg aaucugggag cacuuagaca ccauugauga cgccaagcuc     2520

cggucagaua aauauuuucu guacuucaug cagcuuggcc guuguaugua cacugaaaag     2580

ccuauccccu uugaggaagu cgagaaugaa cauaaaugga acauugacca caucuggcca     2640

caagcaaaga uaaaggauga cagccuggau aauaaagugu ugguaucuuc caacgagaau     2700

gguaagaaaa gugacagcua ucccauuucu gaugacauca gacacuccau ggccgggcuc     2760

uggcauucac uguacaagaa aggccuaauu agugagaaga aguaucagag gcugacccgc     2820

agcacaccuu ucaccgauga cgaacucucg ggauuuaucg cucggcagcu gguggagacu     2880

agacagucca caaaagccgu ugcaacgcuu uugaaggaac aauucccaaa caccgagaua     2940

gucuacguga aagcgggccu ggugucugau uuuaggcagg aaauggggau guuaaagugc     3000

cgagagguca augaccucca ccaugcuaaa gacgccuauc ugaacaucgu ucuuggaaau     3060

guguacaaca cucgcuucac aaaggauccg cugaauuucg ugaagaacaa cgagaaauau     3120

agcauuaaga ucuuucagaa aaauucagac gguaagaaaa ccggcguaau uacacggaag     3180

gucgaaagag ggggagaggu ggcaugggau cccgaaacca guuucgccau cguuaggaaa     3240

augaugucca agaacucuau acguuacgug cgcuacgcuu auaugagaaa gagcggccag     3300

aauggugggu uuuucgacca aaacccucua cggaaaggca agggaauggu cuccauuaaa     3360

cacaaucucu caaucgagaa guacggcggg uaugauaacg ccacuaucag cuuuuucagu     3420

cuggugucua uuaaagacuu uggaguuauc uugcuggcag uggaguuacu cuccgcuaau     3480

aaguuccuga agaacauuag cgaagccaaa gaguacgcgg ccgauaagcu gucgaaagaa     3540

cugaauguaa agauaguccc aaacgacgug gcuuuucccu ugaauaacag guauaaugug     3600

gucaaaauca acgcaguucu cgagcuggau gguuaccgag ugaugauugc cgcuaagcaa     3660

aauucaucua ucaaguucgu aaguaacgaa ucccuaauuc ugagcucuca gcacauggcc     3720

uaugugaaaa agaucacgaa auuuauagag aagcggaaac aggacaaggu cuguaugauc     3780

aacgcauuca gcggcauuuc caucgaggau aauauugaac uuuacgacgu gcucguuuca     3840

aagugcaaca ucaaaccuuu caauauaaug uacgagaacc uggggauuaa guugagugau     3900

aaucgcgaca acuuuaucaa aaagagcgug gaaaaucagg uccugaucuu aucugagaug     3960

gugagacucu ucaaaaccgg aaggaaggcg ggcguuaacc uggaagaggu gggugggcgg     4020

aaaaauuccg gcgucaagac acuuaacacu ugucuguaua agguaaaaga cuacucaaau     4080

auauuuuugg uggaucagag uccaaccgga cucauugagc auaagagccc caaccugcua     4140

gaccuguga                                                             4149


<210>  3220
<211>  4155
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3220
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuaua       60

aucacagaug acuauaaagu cccugccaag aaaaugagag uucucgguaa cacugauaag      120

gaguacauua aaaagaaucu uaucggcgcu cuguuguucg acgggggaaa cacggcagcc      180

gauaggcggu uaaagcgcac cgcgcgacgu agauauacaa ggcggcgcaa uagaauccug      240

uaccuacagg aaauuuuugc ugaggaaaug ucaaaagugg acgauaguuu cuuucacagg      300

cucgaggaca gcuuccuggu agaagaggau aagcggggcu ccaaauaucc aauauuugcc      360

acucuucaag aggaaaagga cuaccaugag aaauucucua ccaucuauca ucugcgcaag      420

gaauuggcag auaagaaaga gaaggccgac cuccgacuga uuuacaucgc ucuggcacac      480

auuaucaaau uuagagguca cuuccucaua gaggaugacu cguuugacgu gaggaacaca      540

gauauuucaa agcaguacca ggacuucuua gaaaucuuua auaccacuuu cgagaacaau      600

gaucugcuua gccagaacgu cgacguggaa gccauuuuga cagauaaaau caguaagucc      660

gcuaaaaagg accggauccu ggcccaauau ccgaaucaga agucuaccgg gauuuuugcg      720

gaguuccuaa aacucauagu uggaaaccag gcagauuuca agaaauacuu uaaucuggaa      780

gacaagacgc cccuucaguu cgccaaagau agcuaugacg aggaucugga gaacuugcuc      840

ggccaaaucg gcgacgaauu ugcugaccug uucuccgccg caaagaaguu auacgauuca      900

gugcugcuca gugggauucu uacugucauc gaccugagca ccaaagcucc uuugucugcc      960

uccaugauac agcguuauga ugagcaucgc gaagaccuaa agcagcugaa acaguuugug     1020

aaggcgucac ucccagagaa auaccaagaa auuuucgccg auagcaguaa ggacggauau     1080

gcagguuaca ucgagggcaa gacaaaucag gaggcuuuuu acaaauaucu gucuaagcug     1140

cugacuaaac aggaagauuc cgagaacuuc uuggaaaaga ucaaaaacga ggacuuucuc     1200

agaaagcaaa ggaccuucga uaaugggagc auuccccacc agguacaccu gacagaauua     1260

aaggccauca uucggcgaca gucggaguac uauccuuuuc ugaaagagaa ccaggaccgc     1320

auagaaaaga uccuaaccuu caggauucca uacuauaucg gaccccucgc uagggagaaa     1380

ucugauuuug ccuggaugac gcggaagacu gacgauucca uccgcccuug gaauuucgaa     1440

gaccugguug acaaagagaa gucagcagag gccuucauuc auagaaugac aaacaaugau     1500

uuuuaccuuc cagaagagaa aguguugccg aagcacaguc ugauauauga aaaguucacc     1560

gucuacaacg agcucacuaa agugagguac aagaaugaac aaggcgagac auacuuuuuc     1620

gacagcaaca ucaaacagga gauuuuugau gguguguuca aggaacaucg uaaagucucc     1680

aagaagaaac ugcuugacuu ucuggcuaag gaguaugaag aguuccggau cguugaugug     1740

auuggguugg acaaagaaaa uaaggcguuu aacgcaucuc ucggaaccua ucacgaucug     1800

gagaaaaucu uagacaagga uuuccuggac aaucccgaua acgagagcau acuagaagac     1860

aucguacaga cgcucacccu guuugaggau cgagaaauga uuaagaaaag acuugagaau     1920

uacaaggacu uguucacaga aucacagcug aaaaagcucu auaggcgcca cuacacuggc     1980

uggggccggc ugagugccaa auuaaucaac gggauuagag acaaggaguc ccaaaagacc     2040

auccuggauu aucuuauaga cgauggaagg agcaaucgca acuuuaugca guugauuaac     2100

gacgaugguc gaucuaaucg gaacuucaug cagcucauca augacgaugg ccugucauuc     2160

aaaaguauua ucagcaaggc ucaggccggg ucccauucug acaaccuaaa agaggugguc     2220

ggagaacugg caggcucgcc ugccauaaag aaagguaucc uccaaagccu gaagauugug     2280

gaugagcuug uuaaagugau gggguacgaa ccagagcaga ucgucgugga gauggcucgu     2340

gaaaaucaga caacuaacca gggcagaaga aauucccgcc aacgguacaa guugcuggac     2400

gauggaguaa agaacuuagc cucagaccuc aauggcaaca uucugaaaga guaucccacc     2460

gacaaucagg cacuucagaa cgaaagacug uuuuuguacu aucuccaaaa ugggagggau     2520

auguacacag gagaggcgcu ggacaucgau aaccuaaguc aguaugacau agaucacauu     2580

aucccucagg cuuucaucaa ggacgauucu auugacaauc gaguucuggu gagcuccgcc     2640

aaaaaccgcg guaagucaga ugacguccca agccucgaaa ucgugaaaga uugcaagguc     2700

uuuuggaaga aacugcuuga cgcaaaguug augucucagc ggaaauacga uaaccugacc     2760

aaggccgaga gaggcggguu aacuuccgac gacaaagcua gguucauuca acgucagcuc     2820

guggagacgc gccagauaac aaagcauguu gcccggaucc uggaugaaag auuuaauaac     2880

gagcuugaca guaagggaag gcggauucga aaagugaaga ucguaacccu gaaaagcaau     2940

cuagucucua acuuccgcaa ggaauuuggc uucuauaaaa uuagagaggu gaauaacuac     3000

caccaugcac acgaugcuua ccucaaugcc guuguggcga aggccauccu gacuaaguau     3060

ccccaguugg aaccggaguu ugucuacggu gacuauccua aauacaacuc cuauaagacc     3120

aggaaaucag cuacagagaa gcuguucuuu uacaguaaua uaaugaacuu cuucaaaacc     3180

aaggugacuu uagcagaugg gacaguagug guuaaggacg auaucgaagu caauaacgac     3240

acgggcgaga uuguguggga uaaaaagaaa cacuuugcca ccgugcggaa gguccucagc     3300

uauccccaaa auaacaucgu uaaaaagacu gaaauucaga caggaggcuu cucgaaagag     3360

uccauccugg cucauggaaa uucugacaag uuaauaccac gaaagaccaa agauauuuac     3420

cuggacccua agaaauacgg ggguuuugau agccccaucg uggccuauuc aguguuggua     3480

gucgcagaca ucaagaaagg caaggcgcag aagcucaaaa cagugacuga acugcuaggg     3540

auuaccauaa uggagagaag ucgcuucgag aagaacccau ccgccuuucu ggaaucuaaa     3600

ggauaccuua acaucagggc ugacaagcuc auuauccugc ccaaauauag cuuguucgag     3660

cuggaaaaug gccgucggag auuacucgcc uccgcuggug agcugcagaa ggggaacgag     3720

cuugcacugc cuacgcaauu uaugaaguuc uuguaccucg ccucaaggua uaaugaaagc     3780

aaaggcaagc cagaggaaau ugagaaaaag caggaauuug uuaaccagca cgugaguuac     3840

uucgaugaca uccugcaacu aauaaaugau uuuucuaaac gcgucauccu ggcagacgcc     3900

aaccuugaga agauuaauaa gcucuaucag gauaacaaag agaauaucuc cguggacgaa     3960

cuggcuaaca auauuaucaa cuuguucacc uuuacaagcc uuggagcgcc ggccgcauuc     4020

aaguucuuug auaaaauagu ugaccgaaag agauacacuu cgaccaaaga ggugcuuaau     4080

ucaacacuca uucaucaguc uaucacuggc cuguacgaaa cccggaucga ucuugggaag     4140

cugggagagg acuga                                                      4155


<210>  3221
<211>  4155
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3221
augaagcuga gaggcaucga ggacgauuac agcauugggc ucgacauggg aaccuccucu       60

guggguuggg ccgucacaga ugaaaggggc acucuugcuc acuucaaacg gaagcccacg      120

ugggggucac gccuguuucg agaggcacag accgccgcgg uugcucguau gccuagagga      180

caaaggcggc gcuaugugag aaggcggugg cgcuuggacu uacugcagaa acuauucgaa      240

cagcagaugg agcaagccga uccagacuuu uucauacgac ucagacagag uaggcugcuu      300

cgggaugacc gugcagaaga acaugccgau uaccgcuggc cgcuguuuaa cgacugcaag      360

uucacagaga gagauuauua ccagagguuu cccacuaucu aucacguacg gagcugguug      420

auggaaaccg acgagcaggc ugauauucga cucaucuacc uggcacugca uaauauugug      480

aaacaccgcg gcaacuuccu cagagaaggu caauccuuau cugccaaguc ggcuaggccu      540

gacgaggccc ugaaucaucu ucgggagaca uugcgcgucu ggucaagcga aagaggguuu      600

gaguguagua ucgcggacaa cggauccaua cuggcaaugc uaacccaccc agaucucucu      660

cccagcgaca ggcguaagaa aaucgccccu cuguucgaug ugaaguccga cgaugcugcc      720

gcagacaaaa agcuaggcau ugcucuggcc ggcgcgguua ucggguugaa aacugaauuu      780

aagaauauuu ucggagauuu uccaugcgag gacucaagua ucuaucucag caacgaugaa      840

gccguggacg caguccgguc ugcuuguccc gaugacugcg ccgagcuguu cgaccgauua      900

ugugaggugu acuccgcuua cguacugcag ggucuccuuu cauaugcccc uggccagaca      960

auaagcgcaa auaugguuga aaaguacaga agguaugggg aggaucuggc cuugcuuaaa     1020

aagcugguga aaauuuacgc uccagaccag uaucgcaugu ucuuuagugg agcgaccuac     1080

ccgggcacgg guaucuauga ugcagcccaa gcucgggggu acacuaagua caaccucgga     1140

cccaaaaagu cugaauauaa accuuccgag agcaugcagu acgacgauuu cagaaaggcc     1200

gucgaaaagc uguuugcaaa aaccgacgcc agggcugaug agcgcuaucg aaugaugaug     1260

gaccgguucg auaagcagca auuucuucgu agacugaaaa caucggacaa uggcucuauu     1320

uaccaccagu ugcaucucga ggaacugaag gccaucgugg agaaccaggg cagguucuau     1380

ccauuuuuaa aacgcgaugc agacaagcug gugucccuag ucucauuccg gauccccuac     1440

uauguugggc cucucaguac uagaaaugcg aggaccgauc agcacggaga aaaccgauuu     1500

gcuuggagcg agcgcaagcc agguaugcaa gacgaaccca uuuucccgug gaauugggag     1560

uccauaaucg accggucuaa aagcgccgag aaguuuauuc ugagaaugac aggcaugugc     1620

accuaccuuc agcaggaacc uguguugccc aaaucaaguc ugcucuacga ggaguucugu     1680

guacugaacg agcuuaaugg ggcacauugg uccaucgaug gagacgauga gcacagguuu     1740

gacgccgcug aucgugaagg cauaauugag gaacuguucc gccggaagag aacggugagc     1800

uauggugacg ucgccgggug gauggagagg gaacgaaacc agaucggcgc acacgugugc     1860

ggaggccaag gggagaaagg auucgagucu aaguuggguu cauacaucuu uuucuguaag     1920

gauguuuuua aaguggaacg gcucgagcag agugacuauc caaugauuga acgcaucauu     1980

cuguggaaca cuuuauucga ggauagaaag auacugagcc agaggcuaaa agaagaguac     2040

ggcucccggc ucucugcuga gcagaucaag acaauuugca aaaagcgauu uaccgggugg     2100

ggaagacugu cggaaaaguu ccuuacuggc aucacagucc aaguggacga ggauagcgua     2160

uccaucaugg acguuuugcg cgaagguugu ccugugucag ggaaaagggg ccgugccaug     2220

gucaugaugg agauucugcg ggaugaggaa cucggauuuc agaagaaagu ggacgacuuc     2280

aauagagcgu uuuucgccga gaacgcucag gcacugggcg ucaaugaauu acccgggagu     2340

ccagccguga ggcgcucucu gaaccagagc auacgaaucg uugaugagau ugcuuccauc     2400

gccggaaagg cacccgcgaa uauuuuuauc gaagugacca gagacgagga uccuaaaaag     2460

aaaggucgga ggacgaagcg ccguuauaac gaccuuaagg augccuugga ggcuuucaaa     2520

aaggaagacc cagagcucug gagagaacug ugcgagaccg ccccgaauga uauggacgaa     2580

cggcuaucac uguacuuuau gcaaaggggc aaaugccucu auagcgggcg cgcaauagau     2640

auccaucagc ugucuaacgc uggaauuuac gagguagacc acaucauucc ccgaacauac     2700

gucaaggaug acucccuuga gaauaaagcc uugguguaua gagaagagaa ccagcggaag     2760

acugacaugc uguuaaucga uccugaaaua aggcgcagaa ugaguggcua cuggaggaug     2820

cuccaugagg caaagcugau uggugacaaa aaguuccgga aucuucugcg uagccgcauc     2880

gaugacaaag ccuugaaggg guucauugcu cgacaacucg uugaaaccgg ccagauggug     2940

aaacugguca gaucucuacu ggaggcgagg uauccagaga caaacaucau auccgugaag     3000

gccucaauca gucacgaucu ccggacugca gcugaacugg uaaagugucg cgaggccaau     3060

gacuuucauc acgcccacga ugcuuuccuu gcaugcagag ugggauuguu uauucagaaa     3120

aggcaucccu guguuuacga aaacccuauc ggccugagcc aggucgugcg gaauuaugug     3180

cgacaacagg ccgacauuuu caagagaugc cgcaccaucc cagggucguc cggauuuaua     3240

gucaacucuu ucaugacaag cggcuuugau aaagagaccg gugaaauuuu caaggacgau     3300

ugggacgcgg aggcugaggu ugaagggauc aggcggucau uaaacuuucg ucaguguuuc     3360

aucaguagaa ugcccuuuga ggaucacgga guguucuggg acgccacuau uuacuccccg     3420

agggcgaaaa agacggccgc ucucccucug aagcagggcc uuaauccauc ucgcuauggu     3480

agcuucuccc gggaacaauu ugcauacuuc uuuaucuaca aagcccgaaa ccccagaaag     3540

gagcagacac uguucgaguu ugcacaggug ccuguaaggc uaucagcuca gauucgccaa     3600

gacgaaaaug cccucgagcg guaugcaaga gaacuggcca aagaucaggg cuuggaguuc     3660

auacguaucg aaaggagcaa gauucugaaa aaccaguuaa ucgagauuga cggggaucgg     3720

cucugcauca ccggaaagga ggaaguccgc aaugcuugug agcuggccuu ugcacaagac     3780

gaaaugcgag ugauaagaau gcuuguuagu gagaaacccg ugucuaggga augcgucauc     3840

ucccuguuca accggauuuu gcuccauggc gaucaggcua gccgcagacu gucgaagcag     3900

cuaaagcugg cccuucucuc agaggcguuu ucugaggcca gugacaaugu gcagaggaac     3960

guugugcugg gguugaucgc aauuuucaau ggauccacua acaugguaaa ucugagcgau     4020

aucggcgguu cuaaauuugc ugggaacguc cgaauaaagu acaaaaagga auuagccagc     4080

ccaaaaguga augugcaccu cauugaccaa uccgucaccg gaauguucga gcggcguaca     4140

aagaucggcc uguga                                                      4155


<210>  3222
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3222
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

auaacugaug acuauaaagu uccuucuaag aaaaugaagg ugcucgguaa uacgucaaag      120

aaguacauca agaaaaaccu ucugggcgcu uuguuauucg auagugggau uaccgcagag      180

ggaagaaggc ugaagcggac agcccgccga cguuauacua gaaggcggaa ucgcauccua      240

uaccuccagg aaauuuuuag caccgagaug gcgacacugg acgaugcuuu cuuucaaaga      300

cuugacgauu ccuuccuggu accagacgau aaaagggacu cuaaguaucc gaucuuuggc      360

aacuuggugg aagagaaagu cuaccacgau gaguucccca ccauauauca ucuccggaag      420

caccuggccg acucgucaaa gaaagcagau cugcgccucg uguacuuagc ccuggcucau      480

augaucaagu accgagguca cuuucuuauu gaaggggacu ucaauagcaa aaacaaugac      540

uugcagaaga acuuucagga uuuccuggac acuuauaaug caaucuuuga gagugaucua      600

ucccucgaaa acucuaaaca gcuggaggaa auuguuaagg acaaaaucag caaguccgcc      660

aagaaagaua gaauacuuaa gcuguucccu ggagagaaaa auucaggcau uuuuagugag      720

uucuugaagc ucaucguggg caaccaagcu gacuucagga aauacuuuaa ucuggaugaa      780

aagaccagcu uacaguucuc uaaggagucc uaugacgaag aucuggagac acuccuuggg      840

cauauuggag acgauuacuc agacgucuuu cugaaagcca agaaauugua ugacgcgauc      900

cuacugagcg guauccucac ggugacugau aacggcaccg aagcaccacu gaguucugcc      960

augauuaugc gguacaagga gcacgaggaa gaccuugcuc uguugaaagc cuauauacgu     1020

aauaucuccc ucgagacaua caacgaagua uucaaggaua acacuaagaa uggguacgca     1080

ggauauauug acggcaaaac cagccaggag gauuuuuacg uuuaucugaa gcgcuuacug     1140

gcuggucuag aaggggccga cuacuuccuc gagaaaaucg auagagagga cuuucugagg     1200

aagcagcgga cauucgauaa cggaucgaua cccuaucaaa uuuaccuuca ggaaaugcga     1260

gcgaucuugg acaaacaggc caaguuuuau ccuuuccugg caaagaauaa agagcgcauc     1320

gaaaagauuc ucaccuuuag aaucccauac uacgugggcc cccuggcuag gggcaacucu     1380

gauuucgccu gguccauucg gaaacgcaau gagaagauaa cgccuuggaa cuuugaagac     1440

gucaucgaua aagagucaag ugcugaggcc uucauuaaua gaaugacuag cuucgaccuu     1500

uaucugccag aagagaaggu guugccgaaa cacucccucc uguacgaaaa cuuuacagug     1560

uauaaugagu uaaccaaggu cagguucauc gcagagggga ugcgugacua ccaguuucug     1620

gauucuaagc aaaagaagga caucguucgg cuauauuuca aaggaaagcg aaaagugacu     1680

gauaaggaca uuauagaaua ccuccaugcc aucgaugguu augacggcau ugagcugaag     1740

gggaucgaaa aacaguuuaa cagcucacuu aguacauacc acgauuugcu gaauauuauc     1800

aacgacaagg aguuccucga ugacuccagc aaugaaacca uaaucgagga gauuauccau     1860

acgcugacca uguuugaaga uagagagaug auuaaacaga gguuaucuaa guucgacaac     1920

aucuuugaua aaucaguacu gaagaaacuu agucgccggc acuacacagg auggggcaaa     1980

uugagcgcua agcucauaaa ugguauuaga gacgaaaaau ccgggaacac uauccuggac     2040

uaucuaauug augacggcgu gucuaacagg aauuucaugc aacugaucca cgaugacgcg     2100

cucucguuua agaaaaagau aaagaaagca cagaucauug gagauaagga caacaucaaa     2160

caggucguua agagccugcc cggcuccccu gccauuaaaa aggggauccu ucagucaaua     2220

aaaauugugg augaguuggu gaaggucaug ggacgcgaac cagagaguau cgugguagag     2280

auggcucgag aaaaucaaua caccaaccag gguaagucua auagccagca gcggcugaaa     2340

cguuuagagg aaucccucaa gggccugggg ucaaaaaucc uuaaggagaa cguucccaca     2400

agacugagca aaauugacaa uaacgccuug caaaaugaua ggcucuaucu guacuaucua     2460

cagaacggaa aggacaugua cacuggcgag gaacuggaua ucgaccgccu cucuaauuau     2520

gacauugauc auauaauccc ucaggcauuc cugaaggacg auuccauuga caacaaagug     2580

cuugucagua gcgccucuaa ucgggguaag uccgaugacg ugccaucauu ggaggucgug     2640

aaaaagagaa aaacccugug guaccaguua cucaagagua agcugaucag ccaaaggaaa     2700

uuugauaacc uuacaaaggc ugaacgaggg ggccugucgc aggaggaaaa agccggauuc     2760

auucagcgcc agcuaguuga gacccggcaa aucacuaagc acguggcaag acuccuggac     2820

gagagguuca auaacaaaaa ggaugaaaac aaucguacgu ugcgcacagu aaagauaauc     2880

acccugaaau ccucuuuagu cagccaguuu cggaaggacu ucgagcucua caaagugaga     2940

gaaauuaacg auuuucauca cgcgcaugac gcuuaucuga augccguugu ggcaucagcc     3000

cuucugaaga aauaccccaa guuggagccg gaguucgucu auggcgauua cccuaaguau     3060

aacaguuuua gggaacgaaa auccgcuacu gagaaggugu acuucuauuc uaauaucaug     3120

aacauuuuua aaaagagcau cccccucgcc gacgggaccg uaauagaccg gccacugauu     3180

gaagugaaug aggaaacagg agaguccguu uggaacaaag ucgcagaucu aaauaccgug     3240

cgcaaggugc ugucauacag ccaggucaac aucguuaaaa agguggagga acaaaaucac     3300

ggucuugaca gaggcaagcc uaaaggacuc uucaacgcua aucugaguuc uaagcccaaa     3360

ccaaacucca aggagaauuu ggugggggcc aaagaauacc uggaucccaa gaaguauggc     3420

gguuacgcgg ggaucagcaa cucguuugcc auuuuaguaa aaggcacuau agagaaggga     3480

gcuaaaaaga aaaucacaaa cguccucgag uuccagggca uuucaauccu ggacaggauu     3540

uauuaccgga aggauaaacu uaauuuucug uuggaaaaag gguauaagga caucgaacuc     3600

auaaucgagc ugccuaaaua cucucuauuc gagcugagug auggaucccg aagaaugcuu     3660

gcaagcauuc ucucuacgaa caauaagcgc ggcgaaaucc acaaagguaa ccagauuuuc     3720

aucagccaga aguuugugaa gcuguuguau caugccaaaa ggauauccuc aaccuucaau     3780

gagaaccacc guaaguacgu ugaaaaucau aaaaaggagu uugaggaauu auucuacuau     3840

auucuggagu uuaacgaaaa uuacgugggg gcuaaaaaga acggagagcu ccugaaaagu     3900

gccuuccaaa gcuggcagaa ucacucuauc gacgaacuuu gcuccucauu uaucggccca     3960

acugguagug agcggaaggg ccuguucgag uugacaagca gagggggagc agcggauuuu     4020

gaguuccuag gcgucaagau uccgagguau cgcgacuaca cccccuccuc ucugcucaaa     4080

gaugccacac ugauccacca gucggugacu gggcuuuaug aaacccgaau agacuuagga     4140

aagcugggcg aagauuga                                                   4158


<210>  3223
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3223
augcagaccu acuucggcau gaagaaaauc cuggggcucg accuuggaac aaacagcauu       60

gguugggccc uggugaauga ggcuaacgau gacaaugaaa aguccucuau aaucaaauug      120

ggcgucagag uuaaccccuu aacuguggau gagcaaucaa auuuugaaaa ggggaaaagu      180

auuacgacca acgcagagag gacaaucaag cggagcauuc gccgaucccu gcagcguuau      240

aagcuaagaa gggaaaaucu caucgagaua cugaaagagc acaacuucau cacugacgau      300

accauucuuu cugaagacgg aaaugccucg acauuugaga ccuaccggcu gcgcgcgaag      360

gcugccucag aaaagaucag cuuggaggag cucggcagag uacugcugau gauuaacaag      420

aaaagggguu auaagaguuc ccggaaggca aagaaucagg aagaggggca gcucaucgau      480

ggaauggaag uggccaagga guuauacgaa aagaacauaa cuccuggcca auucgucuuu      540

cagcugcuua agcagggcgg gaaauucauu ccagacuuuu aucgcucuga uuugcaggcu      600

gaguucgaca agaucuggga guuucaaaaa cuguuccaug cagaaauucu aguggaugac      660

uuuaagaagc agcucgagcg aaaaggaaag cugaauagcu ccaaaaucuu ccuugguaga      720

uuuggcaucu acacagccga aaacaagggg aaugauaaaa ggcugcaggc uuaucagugg      780

cggguugacg ccuugaccaa ggagcucacg aaggaggaac uggcguacgu gauuucagac      840

uuaaacggaa uaaucaguuc aucuuccggc uaccuggguu caauuagcga ucguaguaaa      900

gagcucuauu ucaacaauca aacugucggg caguuccuua uggcacagcu ggaccacucu      960

ccgcauaccu ccuugaagaa ccaaguguuu uaccgccagg auuaucuaga cgaguucaau     1020

acacuguggg aaaaccaggc ccacuaccau aaaguacuca cuagcgaucu gaagucggaa     1080

aucagagacg uuauaaucuu uuaucagagg cggcuuaaaa cccaaaaggg acugauuucu     1140

uucugcgagu uugaaucccg aaagaucgag guggagauug auggcaaaaa gaaaaucaag     1200

aaugucggcu cacgcgugug ucccaaaagu agcccuauuu uccaggaauu uaagauaugg     1260

cagaucuuga acaauauuga gcuccuggaa aaguccucua aagcuaagag auucuuagac     1320

caggaggaga aagaacugcu auuuaaggag cucagcauca aaucaaagau caguaaaucc     1380

gaugcccuga aacuuuuguu caagaacagc agggaacugg accucaauua caaagagauu     1440

gaagggaacc ggacacaauc ugcacuguuu gaugcuuauu caaagauaau cgagcuuagu     1500

ggacacggug aguacgacuu cagcaaaaug gaagccucca auauuaucga gcuggugcag     1560

gaagucuuua ccggcuugaa cuacaauacg gagauucucu auuucgacuc uucgcuggaa     1620

gggaaggauu ucgagaacca gccaauguac cgcuuauggc aucugcuaua uagcuuugag     1680

ggagacgauu ccaaaacugg caaugaaaag cucaucaaca agcugucaga gcuuuacggu     1740

uucgaaaaga aguauaguau aguuuuggcg aacgugucuu uucaggagga cuacgggagc     1800

cuguccacaa aagccaucag aaagauucac cccuaucuca aagaaggcau ccaauacgau     1860

guagcaugcg aguucgcugg auacaggcac ucaaagagcu cucugaccaa ggaggaaauu     1920

gagaauaaaa ucuuaaagga ccagcuggaa auacuuccua aaaacuccuu gcguaaucca     1980

guggucgaga agauucucaa ccagaugauc aaugugguua acgcccugaa ugagaguuau     2040

ggcaaacccg augaaauucg gaucgagcua gcucgagaac ugaaaaagag cgccaaagag     2100

agagaggaac ucacugcauc uauaggggag acaaccauca agcaugaaaa auacagggcc     2160

auucuggaga acgaauuugg aaucucccac gugucacgca augacauuau ccgguauaaa     2220

cuuuacuugg agcuggaaaa cagagguuau aaaacguuau acaguaauac cuaugucagc     2280

caggagaaac ucuucucgaa agaguuugau auagaccaca uuaucccuca auccugucug     2340

uucgaugacu cuuuuagcaa caagacacuu gaaucaaaga gugugaauau cgagaaaggc     2400

aacgauacug cuuucgacua cguauuugcg aaguacgggg aagcaggacu guccgacuau     2460

auuauccaua uugaggaauu guucaaagau ggcaagauau cuaaaaccaa gcucaauaag     2520

cugaaaauga aggguaacga gaucccaagc gacuuuauug ccagggauau ccgcgacaca     2580

caguacauug cucgaaucgc caaaaacaua cuagaggaaa cuguucggaa uguggucucc     2640

accucaggga gcaucacaga ucgucugaga gaggacuggc agcucauuga ugugaugcaa     2700

gaacugaacu gggagaagua uagugcacuu ggcuugaccg agaucuacga aaauagggag     2760

ggacacacua uucgccggau caaagacugg acgaagagaa acgaucauag gcaccaugcc     2820

auggacgcuc ugacaauagc cuucaccaag cgaucucaca uucaguauuu aaauaaccuc     2880

aaugcacgcu ccgauaaaag cggcucgauc uacgggaucg aaacuaagga gcuguaucgg     2940

gacgaucacg gaaaacuuaa guucaaaccg cccauuccuc ugaacgaauu uagagcggag     3000

gcuaagaagc aucuagagaa uacccucaua ucaaucaaag ccaagaacaa agucgcaaca     3060

auuaauauca acaccacuuc uacaaaggaa gguaaaaaua agaagaggca gcugacgcca     3120

cguggccagu ugcaccugga gaccauuuac gggaguaucc aacaguacgu gacuaaacag     3180

gaaaagguug gagccuccuu caacgaggaa cagauaagca aaguggcuaa gcaaaaauau     3240

cgccaggccu uacucaaucg gcugagagag uuugacaacg accccaagaa agcauucaca     3300

ggcaacaauu cuuuugagaa gaacccuauc uaccuugaug uacugcagac ccagaagguc     3360

ccagacaaag uggaaacagu uagcaaggag auuaucuaua cuauuaggaa agauaucacc     3420

cccgaauuga agauagagaa agugauugac aagaagauca aaacgauccu ccaaucccga     3480

cugaaugaau acucagguga uaguaagcgg gcuuucagca accuaaaaga gaauccgauu     3540

uggcugaacc aggagaaggg gaucgaaaua aaacgcguca ccauuucugg caucuccaau     3600

gccguggcga uucaugacaa gaaggauaaa aacggaaauc uuauccucga caacgagggc     3660

ucaacacagg ccguagauuu ugugaauacu gggaacaauc accacguugc uauauauaag     3720

gacgaaagag gaaaccugca ugagaguguc gugagcuucu uugaggcaug caccagggug     3780

aauuugggcg guccugucau uuucaaaaac uacaauaagu ccgaugggug ggaauuucug     3840

uucucuauga aacaaaacga guauuucguu uuucccaacg aaaagacagg auucaaucca     3900

aacgagaucg acuuacucga cccugauaau uaccggcuga ucucgcccaa ccuuuuucga     3960

gugcagaaga uuagcagcaa aaauuacgug uucaaccacc aucuggaaac uuccgccauc     4020

accggcgaga cguugaagaa uaaaaagcag cucaguucug uaacauauca cuuuauucag     4080

agccugccac ccauaaaaga caucgucaag gugagaauua acaauaucgg ucaaaucguu     4140

ugggugggcg aguacuga                                                   4158


<210>  3224
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3224
auggacgagc ugcagaacuu cgaaaagggc aaaagcauca ccacaaaugc cgauagaacu       60

gcugggaggu ccaugcggcg caaccuccaa cgauacaaac uucguagaga gaaucugauu      120

gaaguguuga aagaguauaa cuuuauaacg gacgaaauga ucuuaucuga gaauggaaac      180

aggaccacau ucgagacuua ccggcugcgc gcaaaggccg cggucgaaga gauuucacua      240

gaacaguuug cuagaguucu ccugaugauc aauaaaaaga gggguuauaa gaguagccgg      300

aaagccaagg gcgcagagaa cgggacccuu auugauggaa uggagguggc caaaaagaug      360

uacgacgaag gcaucacacc cggugagcug ugcuugcagc uccugaccgc ugggaaacgc      420

uauuucccug auuuuuaccg auccgaccug caggcagagu ucgauagaau auggaauuuu      480

caaaagcagu ucuauccaga cucucucauc gauaagguaa aagacgaggu gaggggaaag      540

aacaaaucgc agacuugggc cauuuuagcu aaguacuuug ucuggaaaga aguggaaaau      600

ucauggaacg aggaagaggc ccagacacgg cguguugaaa aggaguaccg ccuggugggc      660

aucaaaagag gcgucaagag ggaagaacuu aaguuggaga auuuccaaug gcgggugaaa      720

gcgcugagcg aacgaaugaa cccggaggaa cuagcaauug uacuccagga gaucaaugaa      780

cagauaagua acuccucugg guaucuggga gccauuagcg aucgcuccaa ggagcuuuac      840

uuuaacagac agaccguugg ucaauaucag auggcugugc uggacaauaa ccccaauauc      900

ggcuugagga acauggucuu cuaccggcag ggguaucucg gagaguuuaa uacgauuugg      960

gaaaaacaag ccgaguucca caaggaacug acugaggauc ucaaaaagga gauccgcgac     1020

aucgugauuu ucuaccagag aaggcugaag ucacagaaag gccucauaaa caucugugaa     1080

uuugagcguc ggcagauuga aguggagauc gacgguaaga aaaagauaaa aaccauuggg     1140

agucgaguca ucccuagaag cucuccacuu uuccaagaau uuaagaucug gcagacacug     1200

aaugauauug agguuuccgu guugggagua aagaacaaaa ggaagaaaca ggacgauaau     1260

ucaacuaccc uacuggacag cgcagagaac aucgauaguc ucaagcugaa ugugucucgc     1320

ccccuugacg cugaugaaaa aucccuguug gccaaggagu uauucauucg ggacaagcug     1380

acaaaaagcg augucuuaaa gcugcuauuu aacaauccuc agaaccucaa ucugaacuuc     1440

aaaaacauag acggcaauag aaccggcucg gcgcuuuauc aagccuuuuc uaagaucuug     1500

gaaauuuccg ggcaugaguc aaucaacuuc aaaaagagug cagaugaaau cguggagcag     1560

guuaaaacga uuuuuagcgc ucugggaugg aauacugagg ugcucugcuu cgacuccgaa     1620

aaggagcugg acaagcagcc auacuuuaaa cuuuggcacc uguuguacuc uuucgaaggu     1680

gauagcacac ccaccggcga cgggaaccuc auacagaaga ucgccgaucu guguggauuu     1740

gagaaagacu augcuucaau uuuagccaau aucacuuucc aagaagauua cggcagucug     1800

uccacaaagg caauuaggaa aauccuaccu caucucaagg acgguaacca guaugauguc     1860

gccugcgagu acgcugggua uaagcacagc aaaucuucac ugaagaaaga ggaaauaaag     1920

aauaaagugc uuaaggacaa guuggagcug cucccaaaga acagucugcg caauccggua     1980

guugaaaaga ucuuaaacca gauggugaau gucauuaacg cgaucauuac cacguacggc     2040

aaacccgaug agauccgaau agagcuggca cgggaacuua agaaaaaggc caagaaacgu     2100

gaggaauuga ccaagacaau uguggacacu accaaaacac augagggaau cagagcuauu     2160

cuccagaaug auuucggcau gagccacguc uccaggaacg ccaucauacg cuauaagcug     2220

uacgaggagc uaaaagacaa uggguacaag acucuguauu cuaacaccua caucccuaaa     2280

gagaagcucu uuucgaagga uuucgacauu gaacacauca uuccacaagc acggcuguuu     2340

gacgauagcu ucuccaacaa aacacuugag gugaagucaa ucaauauaga aaaaggaagu     2400

aagaccgccu augacuuugu ugaggaaaaa uacggugagc agggcuugca ggaguaucug     2460

aacagaugug aaguguuauu cagggauaag aagacuaaac uccgaaagcu gaaaaugcaa     2520

gagucugaaa uucccgaggg guuuaucgac cgcgaucuuc ggaauacgca guacaucgcu     2580

aagaaagccc ugagcauguu gaacgaaauu ugcagaaggg uagucgcaac aaccggagcg     2640

aucacugacg agcuccguga ggauuggcag cugguggacg uuaugaagga acuaaauugg     2700

gagaaguaua aagugcuggg ccucgucgaa uacuucgagg auaacgacgg ucgccugauu     2760

gggcggauaa aggauuggac caaaagaaau gaccauaggc accaugcuau ggaugcccuu     2820

acaguggcau uuaccaagga cguauucauc caguacuuua acaauaaaaa cgccuccuug     2880

aagccugaca cuaaugaggc ugccauucga aacaaguauu uccaaaaugg caaagcaauc     2940

ccacccaugu cacuggauga auuucgggcu gaggccaaga aacacuuaaa cgacacacuc     3000

auuagcauca agacgaaaaa uaaggugguu accauaaacc ugaaccgcac uaagaaaaag     3060

aauggaauca acaaaaagca gcagcagaca ccgagaggcc aacuucaccu ggaaaccguc     3120

uacgggucuc auaaacagua uguggugaag gaggaaaaag ucaaugcguc cuucgaugcc     3180

gcuaagauug caacaguuaa caagccugcc uacagggacg cucuacuccg gcgacugcag     3240

gagaauggaa acgauaguaa aaaggccuuc acugguaaaa auagcuugga caagcagccc     3300

guguggcugg augcaaaaca aaacgagugu gugccagaaa agguaaagac cgucauguua     3360

gagacggugu auaccguuag aaaaccuauc gacgaaaagc ucaaugugga uaaagucgug     3420

gacgcgcgca uuaggaagcu gcuucaggau cguaucaaag aguacggcaa cgccaagucu     3480

gcuuuugcca aucuggacga aaaccccaua ugguugaaua aggagaaagg gauuacaauc     3540

aagcggguuu ccgugucagg aaucaguaaa gcacagagcc uccacgagaa gaaagacaag     3600

gauggcaacu ucauucugga caaggauucg agaaggauac caguagacuu ugucaauacu     3660

gguaacaauc aucacguggc uaucuaucgc aaacccgugc uagauaagaa agggcaagcc     3720

gucuucgacg aaaacggcga gcugaaguuu gaacuugagg aaguuguggu auccuucuuu     3780

gaugcaguga cccgagucaa ccucucucag ccuauuaucg acaaagauua caagagaagc     3840

gagggaugga aauuccuguu uucaaugaag cagaaugagu acuucguguu uccaaacgaa     3900

aagacaggcu ucgacccgaa agagauugau uugcugaauc ccgaaaacua ugccuuaauc     3960

aguccuaauc ucuuccgggu ucagaagaua gcuuccaaag acuacguguu uaggcaucac     4020

cuggagacug ucguggcgga ucgcaaggag cuucaagaaa ucaccuggaa acguauuucu     4080

agccugucca ucuugaacag aauuaucaag guucggguga aucacauagg gcagauuguc     4140

augaucggag aguauuga                                                   4158


<210>  3225
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3225
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

auaacugaug acuauaaagu uccuucuaag aaaaugaagg ugcucgguaa uacgucaaag      120

aaguacauca agaaaaaccu ucugggcgcu uuguuauucg auagugggau uaccgcagag      180

ggaagaaggc ugaagcggac agcccgccga cguuauacua gaaggcggaa ucgcauccua      240

uaccuccagg aaauuuuuag caccgagaug gcgacacugg acgaugcuuu cuuucaaaga      300

cuugacgauu ccuuccuggu accagacgau aaaagggacu cuaaguaucc gaucuuuggc      360

aacuuggugg aagagaaagc cuaccacgau gaguucccca ccauauauca ucuccggaag      420

uaccuggcag acucgacuaa gaaagccgau cugcgccucg ucuacuuagc ucuggcacac      480

augaucaagu aucgagguca uuuucuuauu gaaggggagu ucaauucgaa aaacaaugac      540

auccagaaga acuuucagga cuucuuggau acauacaaug ccauuuuuga aagcgaccug      600

agucuagaga acuccaaaca gcucgaagag aucgugaagg auaaaauauc uaagagcgcu      660

aagaaagaca gaauacugaa gcuguucccu ggagagaaaa auuccggcau cuuuucagag      720

uuccugaagu ugauuguugg caaccaagcc gauuucaaaa aguauuuuaa ucucgacgaa      780

aaggcgccac ugcacuucag uaaagaaagc uacgaugagg acuuagaaac ccugcucggg      840

uauaucggag augacuacuc ugauguguuu cuuaaggcaa agaagcugua ugacgccauc      900

uugcuauccg guauucugac ggucacugac aacggcaccg aggcuccccu cucaagcgcc      960

augauaauga gguacaaaga gcaugaagag gaucuggcac uucugaaggc uuacauccgg     1020

aauauuaguu uggaaacaua uaacgaggug uucaaggacg auacuaaaaa cggguacgcc     1080

ggauauaucg acggcaagac cucucaggaa gauuuuuacg uacaccucaa aaagcuguua     1140

guuggucugg agggggcgga cuauuuccua gagaaaauag aucgugaaga cuuucuccgc     1200

aagcagagaa cauucgauaa uggauccauu ccuuaccaga uccaucugca agagaugagg     1260

gccauccuug acaagcaggc aaaauuuuau ccauucuugg cuaagaacaa agaacggauu     1320

gagaagaucc ugaccuuucg cauucccuac uacgugggcc cucucgcccg aggcaauagc     1380

gauuucgcuu ggucgauaag aaaaaggaac gaaaagauca cgccauggaa uuuugaggac     1440

gucauugaua aagagucuuc cgccgaagca uucaucaacc ggaugacuuc auucgaccug     1500

uaucuuccgg aggaaaaggu gcugcccaag cacaguuugc ucuacgagac auuuaaugug     1560

uauaacgagc ugaccaaagu ccgcuucauc gccgaaagca ugagagacua ccaguuuuua     1620

gauuccaagc agaaaaagga cauuguuagg cuguauuuca aaaauaagcg ucgggugacu     1680

gauaaggaca uaaucgagua ccuacacgcu auugaugggu augacggaau cgaacucaaa     1740

gguauugaga agcaauuuaa cucuagccug ucaacauacc augaucuuuu gaauaucaua     1800

aacgacaaag aguuccugga ugacaguucc aaugaagcga ucauugagga aaucauucac     1860

acccucacga ucuuugagga ucgagaaaug auaaagcaga gacugagcaa auucgagaac     1920

auuuuugaca agucuguauu aaagaaacug ucaaggcgcc acuacaccgg cugggggaaa     1980

cuuagugcaa aauugaucaa uggcauucgg gaugaaaaga gcggaaacac aauccucgac     2040

uaucugauag acgauggugu guccaacaga aauuucaugc agcuaaucca ugacgaugcc     2100

cugucuuuua aaaagaagau uaaaaaggcu caaaucauug gggacaaaga uaacaucaag     2160

caggucgugc aggcccuccc uggcaggcca gcaauuaaaa agggcauucu gcagucgauc     2220

aaaaucguug acgagcuugu gaaggucaug ggacgccgac ccgagagcau ugugguagaa     2280

auggcccggg agaaucaaua cacuaaccag gggaagucca auucacagca gcguuugaaa     2340

agacuggaag agaguuuaaa ggaacucgga ucuaaaaucc ugaaggagaa cauuccugcu     2400

aaacuuagca agauagauaa uaacgcccug caaaaugaca gguuguaucu cuacuaucug     2460

cagaacggua aggauaugua caccggcgag gaacuagaca ucgaucgccu guccaauuau     2520

gacauugacc acaucauucc acaggcauuc cucaaagaua acucaaucga caauaagguu     2580

cuggugagcu cugcguccaa ccgggggaaa agugaugacg uccccagccu ugaggugguc     2640

aagaaaagaa agacauugug guaccagcug uuaaagucua aacucauauc ccaaaggaag     2700

uuugauaauc ugacuaaagc ugaacgagga ggccuuucac cggaggccaa ggcagguuuc     2760

auccagcgcc agcuggugga gacccggcag auuacaaaac auguugccag acuacucgac     2820

gaaagguuca acaacaagaa ggaugaggac aauaaaaccc ugcguacugu gaagaucauu     2880

acguugaaaa guagccuggu aucgcaauuu cgcaaggauu ucgaauuaua caaaguccgg     2940

gagaucaacg acuuucacca ugcucacgau gccuaucuca augcaguggu ugcuuccgcc     3000

cugcuuaaga aguacccuaa acuggaaccc gaguucgugu auggggacua cccaaaguau     3060

aacucuuuua gagagaggaa aagcgcgaca gaaaaggucu acuucuauuc aaauauaaug     3120

aacauuuuua aaaagaguau cuccuuggcc gauggcaccg ugaucgagcg accucucauu     3180

gaaguaaaug aggaaacugg agagucugug uggaacaaag uugcugaccu gaauaccguc     3240

cggaaggugc uaagcuacuc ccaggugaac auagucaaga aaguugagga acagaaucac     3300

ggccuggacc gcgggaagcc caaaggacuu uucaacgcaa aucucucaag caagccaaaa     3360

cccaacagua aggagaaucu ggugggugcc aaggaauacu uggauccuaa aaaguauggc     3420

ggguacgcug gaaucucuaa cuccuuugcc gugcugguaa aaggcacaau ugagaagggu     3480

gcaaagaaga agaucaccaa cauuuuagag uuccaaggga ucagcauacu cgacagaauc     3540

acuuauagga aagauaaguu uaauuuccug cuugaaaaag gcuacaagga cauugaucug     3600

aucauugagu ugccaaaaua uucacucuuc gaacugucag acggaucucg gcgaaugcua     3660

gcgaguaucc uguccacaaa caauaagaga ggcgagauac auaaggggaa ccagauuuuu     3720

aucagccaga aauacgucaa acuucucuau cacgccaaac gcaucucuaa uacguucaac     3780

gauaaucaua ggaaguacgu ggaaaaccac aaaaaggagu uugaggaacu guucuacuau     3840

auuuuggagu uuaaugaaaa cuacguugga gcuaaaaaga auggcgaguu acuuaagagc     3900

gccuuccagu ccuggcaaaa ccacucaauc gacgaacucu gcaguagcuu uauagguccg     3960

accgggucug agcguaaagg acuguucgag cuuacuuccc ggggcggugc agcugauuuu     4020

gacuuccugg gcgugaagau ucccagauau agggauuaca caccuucaag uuugcuaaaa     4080

gacgccaccc ugauccauca gagcgucaca gggcucuaug aaacucgcau ugaucuggga     4140

aaacuuggcg aggacuga                                                   4158


<210>  3226
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3226
augaagaaca aaaccuggua ccugggccuc ggggugggac ccacaagcau cgguugggcc       60

gcuacugacu ccaauuauaa gcugcugaga aagaacaaga aaagguugug gggcguccgg      120

uuauucgagg aagcaaagac ggccguugau cgccgaaccu uucgugcgga gagaaggcgg      180

cuggcucgca gaaaguggag gauuucucua cucgaggagc uguucgccaa agaaguguca      240

aagaaugacc cuaacuuuuu ccuucggcug aaagagagua aguaccacuu ugaggauaag      300

agcuugauga augaauauau acucuucaac gacaaggauu acacagacaa ggauuauuac      360

aaagaguauc caacuaucua ccaucugcgc cugucccuca ccacagaaga gaauccggac      420

auucgaaagg uauacuuagc agugcacucu augcugaaaa acagagggca uuuucuuuug      480

cagggacaau cguucaauga uucaaacauc gacagcguca uuaaggagcu gcuagaacuc      540

gagaccguga aaaucaauuu ugaaguuacu gaugagguga uacacagucu gaagaaaauc      600

gcccuugaaa agaagaaccu guccgacaaa aauaacgaug ucaagaaauu guaucccaag      660

gagaaacagc uccaggagau uuucaagcug guguuuggcg guaagacauc uuuagacaaa      720

cuguacgaca ucgaagagua uaaggaacuc gauaauagcg uaaaauccau uucauucaag      780

gagaaaguuu acgaagaggu gaggccugac uaugagcaga uccuuaguaa cuacauagaa      840

cuguuggauc uagcuaagcu ggucuaugac agcauugugc ucgcagauau caagaaagag      900

gggaagacca uuucugaauc caaaguggag cuguacaaca agcauaaaga cgaucuugcc      960

aaucugaaga aguuggucaa aaucgacuca aagcucagcg aagauaaaaa gaaagagguu     1020

uacuuuacga ucuucaagga ggacaaagaa aagggaacua acuaugugaa uuacacccac     1080

cggagucaag agggcaaguc uugcuccuau gaagauuuca aaaaguuucu gaaaaaggag     1140

uuagagaaac uggaagacag cccaacaaag acuaccauuc uagaggaacu cgaccuggag     1200

acauuccuuc ccuugcagcg uaccaaggau aacucgguag ugccuuacca gauacacaaa     1260

gaggaacugg ucaagauccu cgacaaugcu gccaaauauc auucuuuucu ggcggagaag     1320

gaugacuccg gcuacucaac gcgcgaaaaa gugguucagc uuuucgaguu uaggauucca     1380

uauuacaucg ggccccugaa cgcacaccau aauacugaaa acggagguua caguugggcc     1440

auaagaaaga agggcaugga gagcgugccu gucacaccau ggaauuucaa agaugugauu     1500

gacgaauccg cuucugccga ggcauuugua aacaauuuga agaacaaaug ugcuuaucuc     1560

agcggggagg auguucugcc gaaaaauuca uuacuguaca gugaauaugc ccuacucaac     1620

gagcugaaug cgcuucggua cgaaggaaac cgaaucuccg uggaggcccg cgacgucauc     1680

gugaccaaau uguucaagga acaaggcaag aaagucacua agaaaacaau uaaggagcug     1740

cucaaaaccg aggguuuuau cgaugggaag ggagaaauug ugggcauaga caacaucguu     1800

aaaaaugauc uuaagagcua uagagacuuc aagaaaauuu uagcagauaa guuugacaaa     1860

gagagggugg aaaacaucau ccuguggauu acgcuuuacg gcgagucucg gaaguugaua     1920

aaagagaaga ucaccaaggu auauggggac uucuacacag ucgaugaaau ugagaaaaug     1980

ucacgccuca cuuacaagga cuggggaauc cugaguagaa aacuacugac cgaacucgug     2040

agcgagaagc uguauaauga agagacaggu gagugccuua acauuaucgg cgcuaugagg     2100

caggggacua uauuguuuau ggaacuguua gccgauaaau ucgacuacau gucccagauc     2160

aagaaucaca acaaggagaa uaaagaugac auuaccgaaa ucacacccga uauucucgag     2220

aaccugcacg cuucuccugc cguuaagcgu ucgaucuggc agaccgugcg gauagucgag     2280

gaacuuaaaa cuauuaucgg augugugcca acgaagauuu ucaucgagac aaccaaacga     2340

aauaacgcac ccuauaaggu accuacucug aauagaaacg acuuggugac caaguacaaa     2400

acaauaaggg aacaugagau cuuugaacuc gagaaggaac ugaaaaauac ccuagauuuc     2460

acucugaaca agaaucgccu cauuaacgag gagccaagca aacugaaggc cacaaaacuu     2520

uauuuguacu auacgcaacu gggccggugc auguacaccg guaaaaagau cgacuuaggg     2580

gaacucuuug auaacaauaa caaauacgac auugaucaca ucuaucccca guccaagguu     2640

aaagacggcu cauucaauaa cauaguccug gugacuaagg cuaguaauga caugaaagcg     2700

gauguguuuc cgcuuaaguc uaaguuccag acaaaagaga acaagaucuu uuggagauuc     2760

cugaaagaaa agaaacuaau uaccgaggag aaguacaaua ggcucguccg cacagaagag     2820

uuuagcaacg gagaacugac uggcuucauc acccgacaau ugguugagac gucccaggca     2880

auuaagguga ucucaaaugu gcugcgggaa uuaaacccug agaccacaau uuguuauagc     2940

aaagccgaga auguaucuuc cuuucgucag aguuucggga agauaaaaga agcuaacaga     3000

aaggccgaga auaacgaaaa acucaucagg guccaggaga uuaaugacua cuauaacgca     3060

aaggaugccu accugagcau cgugguugga aacguguaug acgucaaguu uacucgcaau     3120

gugcauaacu ucauuaaaaa uaacaaggau gcucggagau acucucuuaa uggucuguuc     3180

uacaccgacg uuaaaaacgc caauacaauc gcaugggaaa uggauaagac ugugcacgua     3240

gucgagaaaa ccaugaacaa uaacaauaua uugaucacga ggcgaacauc cgagcaaaag     3300

ggcgcgcucu augacgcuac ccuguacaag gccaaagugg cagaaaaggc caaagagggg     3360

acuuauuacc cccuaaagac aaccgauuca aaaauuaagg acgugggaaa auauggcggu     3420

uuuacuaaga ucaagauugc uuacuauagu aucuucgaau acacccugga ugggaaaaag     3480

ggcaaacaga agauaacacg cauuguccca aucccuaucu acauuagcca gaacauaaaa     3540

gacgacaagg cccuucucga guaugcacag acgcggcugc ccggaaagga uuugaaaauc     3600

aaguacagaa aacugugcau uggcucguua guuaagauca auaacuuuaa uuauuacauu     3660

gggggaaaaa ccaacaauaa cuucacuuuu gacuccgcug ugcaaguaau ccucgauaag     3720

gacucugaaa gcuauauaaa ggagcuggcc aaauaccuua acuggaagaa agagaauaag     3780

gaugguaaac ugugggaauc aaucacagag gacaagaaca uucaguugua ugauagucuc     3840

gugaagaaaa ugaauaccgg caucuucauu aggaagacac caaacaaaua cacugaacug     3900

cuaacccugg aggucaagaa uaaauuuauc aacauagaca uugaugaaca ggugaaacuu     3960

cuccuggaaa ucuugaauuu acugacgaac aagaaguuca cauccaagcu caaugagauc     4020

gggauuaccg cgucucgugg aguuuguagc uuuaaccugu ccaaucaguc acaauucagc     4080

aucauagaac agagugugac uggcgucuac gagaaagaaa aacuuauuau cgagaagaag     4140

aaagagggua uucuguga                                                   4158


<210>  3227
<211>  4161
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3227
augaaccaga aguacagcau cggccuggac auugggacca auuccguggg augggccguc       60

auaacagaug acuauaaagu ucccgcuaag aaaaugaagg ugcucgguaa cacugauaaa      120

caaucuauca agaagaaucu ucugggcgca uuguuauucg acucagggga gacggccgaa      180

gcgaccagac ugaaaaggac agcucggcgc cgauacacuc guagaaggaa ccggcuacgc      240

uaucuccagg agauuuuugc cgaagagaug augcagguag augaaaguuu cuuucagaga      300

cuggacgaua gcuuccuugu ggacgaggau aagaggggag agcggcaccc uaucuuuggc      360

aauauugcag ccgaagucaa auaccaugac gaguucccaa ccaucuauca ucugcgcaag      420

cacuuggcug auauauccca aaaagcagac cuccgacugg uguaccuggc ccucgcucac      480

augaucaagu uuagagguca uuucuuaauu gaagggcagc ugaaggccga gaacacaaau      540

guucaggcgc uuuuuaaaga uuucguggag gucuaugaca agaccgugga agagucucac      600

uugucggaaa ucacuguaga cgcacuguca auucuaacag agaaaguuag caagaguagg      660

cggcucgaaa accugaucgc ccacuacccg gcugagaaaa agaauacccu uuuuggaaac      720

cugauagccu uguuccucgg ccugcagccc aauuuuaaaa cgaacuucca auuauccgag      780

gaugcaaagc ugcaguucuc uaaggacacu uacgaagagg aucucgaagg ccuucugggg      840

gaggugggag acgaauaugc ugauuuguuu gccagcgcga aaaaucuaua cgacgccauu      900

cugcucuccg guauccugac cgucgaugac aacucaacaa aggcaccucu uagugcuagc      960

auggugaaac guuaugagga gcaucagaag gaucugaaaa aguucgaaga cuuuauuaag     1020

gugaaugccu uggaucagua caacgcuauc uucaaagaca agaacaaaaa gggcuaugcc     1080

ggguacaucg agucuggagu caaacaagac gaauuuuaua aguaccucaa gggcauucug     1140

uuacagauaa augguuccgg ggauuuccug gacaaaaucg aucgcgagga cuuucuaaga     1200

aagcagagga cuuucgauaa cggaucaauu ccacaccaaa uccaucucca ggaaaugcac     1260

gcaauacugc ggcgacaggg cgagcacuac cccuuucuua aagagaauca ggacaagauu     1320

gagaaaaucu ugaccuuccg caucccuuau uacguuggcc cacuggccag aaaggggagc     1380

agguuugcuu gggcggaaua uaaggcagau gaaaagauua cacccuggaa cuucgacgau     1440

auccucgaca aagagaaaag ugccgagaag uuuauuaccc ggaugacgcu gaaugaucuu     1500

uaccugccug aagagaaagu guugccaaag cauucucucc uguaugaaac uuucacagua     1560

uacaacgagu uaaccaaagu gaaguauguc aaugaacaag gagaggcuaa guucuuugac     1620

gccaacauga aacaggagau auucgaucac guguuuaagg aaaaucgcaa aguuacuaag     1680

gacaaacugc uaaacuaccu caauaaggag uucgaagagu uuagaaucgu gaaccugaca     1740

ggucuugaca aggaaaauaa agucuucaac uccagcuugg gcaccuacca ugaucugagg     1800

aagauucuca auaaaucguu ucuggacaac aaggagaacg cacagaucau cgaggauauu     1860

auacagacgu uaacccuguu cgaagaccgu gagaugaucc ggcaacgacu ucagaaauau     1920

ucugauauuu uuacaaaggc ccaguugaag aaacucgaaa gaaggcacua cacugggugg     1980

ggacgccugu ccuauaagcu aaucaauggc auucggaaca aagagaauaa gaaaaccauc     2040

cuggacuacc ucauagauga cgguuaugcu aacagaaauu ucaugcagcu gaucaacgau     2100

gacgcccuuu cauuuaagga agagauugca aaggcgcaaa ucauugggga uguggacgau     2160

aucgcuaaug uaguucauga cuugccgggc agucccgcca uuaaaaaggg aauacugcag     2220

agcgugaaaa ucgucgacga guuagugaag gucaugggcc acaacccugc aaauauuauc     2280

auagaaaugg ccagggagaa ccagacaacu gaucgcgggc gacguaauuc ccagcaacgg     2340

cucaaacugc uucaggacuc ucugaagaac uuggauaauc cagugaacau caaaaauguu     2400

gaaaaccagc aacuccagaa cgacagacug uuccuauacu auauucagaa uggaaaggau     2460

auguacaccg gugagacacu ggacaucaac aaucucagcc aguacgauau ugaccacauc     2520

auaccccagg cuuauauuaa ggaugacuca uuugauaaca gggugcugac caguuccagc     2580

gagaaucgcg gcaaaucuga caacguaccu ucaaucgaag ucgugugcgc ccggaaggca     2640

gacuggauga gacuuaggaa agcuggguug aucagucaac gaaaguucga uaaucugacu     2700

aaagccgagc gcggaggcuu aacggaaaac gacaaggcgg guuucauuaa gcggcagcuc     2760

guugagacaa gacagaucac caaacaugug gcccaagucc uggaugcuag guuuaaugca     2820

aagcacgacg aaaacaaaaa ggugauucgu gauguaaaaa uaaucacucu uaagagcaau     2880

cugguguccc aguuccgcaa ggacuuuaaa uucuacaagg uucgggagau uaacgauuau     2940

caucacgccc acgacgcuua ccuaaaugcc gucaucggga ccgcacuccu gaaaaaguau     3000

ccaaaauugg cgucugaguu uguguacggc gaguucaaga aguaugaugu gagaaaauuu     3060

auugccaagu cggacaaaga aaucggaaag gcuacagcca aauacuucuu uuacagcaac     3120

cugaugaauu ucuuuaagaa ggaagucaaa uucgcagaug gcaccguugu ggagaggccc     3180

gacauagaaa cuuccgagga uggggagauc gcuuggaaca agcagacaga cuucaaaauu     3240

gugcgaaagg uauuaucaua uccgcagguc aacaucguga aaaagacgga aguucaaacc     3300

cauggacucg accgggguaa accucgcggc cuguuuaaug ccaaccccag uccaaagccu     3360

aagcccgauu cuagcgagaa ucuugugggg gucaaaagaa accuggaccc aaagaaauac     3420

ggaggcuaug cagguauuuc caauucauac gccguguugg uuaaggcuau cauagaaaaa     3480

ggggugaaga agaaagagac uaugguacuc gaguuccagg gcauuagcau ccuggauagg     3540

aucacauuug aaaaggacaa acgggcguuc cuacugggaa agggcuauaa agauauuaag     3600

aagauaaucg agcuucccaa auacucucuc uuugaacuga aggacggguc ccgaagaaug     3660

uuggccagua uucugagcac caacaauaaa cgcggagaga uccacaaggg caacgaauua     3720

uucgucccuc agaaauuuac aacucuccug uaucaugcaa agaggauuaa uaacccaauc     3780

aauaaggauc acauagagua cgugaaaaag caucgugacg auuucaaaga gcuucugaac     3840

uacguguugg aauuuaauga gaaguauguc ggugcuacca aaaacgggga acggcucaag     3900

gaggccguug ccgacuucga uucuaaaucc aaugaagaga ucuguacguc auuucuggga     3960

gcugugaaca guaagaaugc aggccuauuc gagcugacca gccuugguuc ggccuccgac     4020

uuugaguucc ucggcguaaa gauuccgaga uacagggauu auacacccuc uagccuguug     4080

aaagacucaa cuuuaaucca ccaaaguauu accgggcugu acgaaacacg caucgaccuc     4140

uccaagcugg gagaagauug a                                               4161


<210>  3228
<211>  4161
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3228
augaccaaga ucacagagga cuacauugug ggccuggaua uagggacuaa cagcugcgga       60

ugggucgcca uggacuccaa aaauacgauc cucagaaugc acgguaagac cgcuauuggc      120

ucucaucuuu ucgaugcagg gaacucagcc gcggacaggc gggcuuuucg cacaacucga      180

cguagaauca aaaggcggaa guggcgccug aaauuguuag aagagauuuu cgauagucac      240

cuggccaagg uugaccccua cuuuuucgca agacuaaagg aaagcggacu cuccccucug      300

gauaccagga aaaauguguc uucgaucaua uauccaacau cacuugagga caagaaauuu      360

uauguagaua acccgaccau cuaccaucug cgguauaagu ugaugacuga agaccgcaaa      420

uucgaucucc gagaggugua ccuggccauu caccauaucg ucaaguauag aggcaauuuu      480

cuguacaaca cacccgugaa ggcuuucgag gcaagcaaaa uugacguuaa ggguagucuc      540

aaaaaguuaa augaacugua cgaggggcuu gauuccgaau uuaacgugga guuggacucu      600

aauaacgccc uggagaucga aaauauaauu agggacaaaa agaucuucaa aauuaacaag      660

gucaaggaga ucaaucagcu acucagccug aaaaccgaag auaaggagcg gacgaaacuu      720

augaaggacg uguccaaaca aaucguaaac gcuauucugg gauauaagac uaaguuugaa      780

accauauugc ucaaagaugu uucaaaggac gaggccgaug acugggaguu caaacugaca      840

gauguggacg cggauaauaa guuugaaaac uuaaucggcg accugaacga gaaugaacag      900

gagauucucc uugucauccg uaaacuggca aacgccauaa cuuugaguaa uauuguggaa      960

gagggcaaga cccuaagcga gucuaugaag gauaaauaca agaaacacuc cgacgaccug     1020

aagcuccuga aacaggugau cucagaucac ccugaccgcg auaaggcuaa gaaacuugcc     1080

cuggcauaug acuuguacgu caacaauaga caugggaaga uccuccaggc uaaagauauu     1140

cuggaaaaga aaaagacauu aagcaaagag gacuucuaua aggaaaucaa gaaaaaccug     1200

gaugacagua aggagucuca agaaauucua gaugccauau cccucgacag cuucaugcca     1260

aaacagagga ccaaugagaa cggaguuauc cccuaccagc ugcaccagcu ugaguuggau     1320

aagauuaucg aaaaucaagg uaaauauuac ccuuuucuga aggaggugaa cccaguaucg     1380

ucucaucuca agcaggcgcc cuacaaacug gacgaacuua uccgguuccg agugccuuau     1440

uacgucggcc cacugauuuc cccgaaugag ucaacgaaga acaaacagac uaagaaaaau     1500

cagaacuuug ccuggaugau acgcaaggag caagggcaga ucacacccug gaauuucgau     1560

cagaaggugg acagaauggc aagugcuaac aaauuuauua agaggaugac cacuaucgac     1620

acauauuugc ucggagaaga uguucugccu gccaacagcu uacuguacca aaaauucacc     1680

gugcuaaaug agcucaacaa ugucaagauu aacggcaaac ggaucuccgu gccacugaag     1740

caggaacuuu auaauaacuu guuuaagaaa aauucuacgg uaaccacaaa caagcugaaa     1800

agcuaccuca aggagaauua uaaacugaua aagguugaaa ucaaagguuu aucagacgag     1860

aagaaguuca acagugggcu gacuaccuac aauaaacuua agaacuugaa aauuuuugau     1920

cagcagaucg acgaucccaa uuacaacaag gacuucgagc gcauuaucga augguccaca     1980

auauuugagg auaaagcuau uuauaaggaa aagcucaaaa cuaucgacug gcugagcgau     2040

agacaaauug acaagcuauc uaaaaucagg augcagggau ggggccagcu gucaaagaaa     2100

cuccugaguc agcuuaccga uaauaacggc caaacaauaa ucgagcaguu gugggacagc     2160

cagaacaauu ucaugcagau ugugaaccaa gccgauuuua aggacgcaau cgccgucgcu     2220

aaucagaacc uguuagugga caccuccguc gaagauauuc ucaaugaggc guacacuucu     2280

ccugcaaaca agaaagccau ccgucaggug guuaaggugg uagacgauau agucaaagcu     2340

acgucgggga aggugccaaa acagauugcc aucgaguuca cacgggacgc agaugacaau     2400

gccaagauca gccaaucccg agcuaacaag cugagaaaag uuuauaagga aaugucaaau     2460

gagcuugcca gugaaaccau uaggaacgag cuggaacgcg uggcaaaaga ucagaaguug     2520

cucaaagaca agcacuaccu guauuucaug cagcuaggac gggaugcgua cacuggugag     2580

cugaucgaca uugauaaucu cgagcaauau gacauagacc acguccugcc ccagucuuuu     2640

aucaaggaug acagccuuga aaacagagug uugguaaaga aggcugugaa uaacggcaaa     2700

uccgauaaug uuccggucaa gcuguacggg aacaggaugg ccaccgacuu aggaaugaca     2760

auucgccaua ugugggagaa auggaaggaa cagggccuca ucaccaaaac uaaguacaac     2820

aaucugauua ucgauccuga caagauaaac aaauaugagu caagcgguuu cauccaccga     2880

cagcuugugg agacaucuca aauuaucaag cuggugucca cgauucuaca gaguaaauac     2940

cccgauaccg aaaucauagu cguuaaggca cgguauaauc auuaccuccg uaaaagacug     3000

aacuuguaua agagcaggga ggugaaugac uaccaccaug ccauugaugc uuaucugucu     3060

gccaucugug ggaacuuacu cuaccaggug uacccauauc ugcgccccuu uuucguauac     3120

ggccaguaua agaaauuuuc cucagaaccu gagaaggaga aaaucauuua cgacaagacu     3180

cggagauaua auuucauaag ucaaaucuuu gaaaacaaag auaaugacau uaucagcagg     3240

gagacaaaga agaaagucuu cgauaagaaa gacauuaucg aaaaacuuaa acgagcauac     3300

aacuauaagu acaugcuggu gucgcgcgag accgaaacac acgaccagga gauguuuaag     3360

augacuguuu acccacgguu guccagagau accaaaaagu cuaggaaucu cauacccaaa     3420

aagaaagaca ugccuacgga aaucuaugga ggcuacacca acaauagcga ugcuuauaug     3480

auuaucgccc guauuaacaa gaaaaagggg ccagaguacc gcguguucgg agucccgaug     3540

cgggagcugg ugaaucuaag aaaggcggag aaaaacgguc acuauaaggc cuaccugaaa     3600

cagguucuug aacccgaaau cauguauuca aagaauggca aaaagaacaa gacaauaagu     3660

uccuuugaga uugugaaagc uaagguaccu uacaaacagg ucauccucga cggggauaag     3720

aaauucaugc uggcaucuag cacuuacgug caaaacgcca agcaguugac ccugucccag     3780

uauucaaugc aagcuaucac agacaauugc gaaaacgaua cugaggagga aaaggccuua     3840

guggaggcau acgacgaaau ucucaccaau aucgauaaau aucugccacu uuuugacaua     3900

aacaaguuca gggagaaacu gcaugcggga cgagagaagu ucgucgauuu gccccucgcc     3960

gagaaaaagg aaacgauucu gcagguucua aauggccugc acgacaacgc ugugaugccu     4020

aagaucaaaa gccuuggucu caguacacca cugggguuua ugcaguucca uaccggcgua     4080

ucuuuguccg aaaaugccaa guuaauuuac cagagcccca cuggacuguu ugagaaacgg     4140

cucaagaucu cggaucugug a                                               4161


<210>  3229
<211>  4164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3229
augagcgacu ccaagaaauu cgagggcgaa aacgugaagu cuggggauaa aaagaccaau       60

aaaaaccacg gaaagguccu gaagucaagu cccaaagaga ucagaaagaa uacagaauac      120

uuuauugguc ucgacauagg cacuagcucc guuggguggg ccgugacgaa caccgauuau      180

gagauccuua aauucaaugg aaagaggaug uggggcucuc ggcuguuuaa cgaagcaucg      240

acagcugagg agcgccgagg ucaucgugua aauagaaggc ggauucagcg cagaagggac      300

cgguugaacu uacuggaaga gaucuucuca gaagagaugg ccaaagugga uccugaguuu      360

uucaugcgcc uaaaggaaag caaguuucac cucgaggaca aagaugaacg aauccugggg      420

gagaguaaua uuauauucaa gggauccgaa gacggcgaua aaaaguacua ugagaaauac      480

ccaacuaucu aucaucuuag acacaagcug augcaaucuu gcgaggacgu cgauauuagg      540

gaauuguaca uggcgcucca ucacaucauu aaauaucggg gccacuuucu guucaagggg      600

gacuuugagg aaaccggaac aagcaucgag aacauaauua ucgaacugcu cgaguuaauu      660

aauaacaauc ugggugugaa gaaauucgag uacaacgaau cccuuaucaa ugaguugaag      720

aacaucguuu caaacaccag uaauacuauu acaaacaaaa agaaacugcu aggcgaauuu      780

uucaauaaga aggaguacaa agcucauaag agccucuuca uacugucuau cggggccucc      840

gugaaaccgg ucgaucuuuu ucuggaagag gaguauaagg acauugacga uaucaaaaac      900

auaucauuca agaauuugga auacgaccag gugcgugaua ccuaugagag cauuuuucuc      960

gacaagaucg aguucaucga uguagcaaaa cgccuguacg acgccacgau uuuacugagu     1020

aucaagcccg aaggaaaaac ucucucugag gcuaagguug auauuuauaa caaacaccau     1080

aaugaccuug aacuguugaa gaaggugcua aaaaccgaua aggagaaaua cgacuccaua     1140

cugaagcucg auaucaaaaa gggcaacaau uaugucaacu acgugaaaaa gagcaagaau     1200

gguauuucgu guucuaaaga agaguuucac aacgcacuga agaaagugcu ugucaagauc     1260

gaaaagacag acgacaucaa guacauucug aaugagauag aguuggaaag auuccucccu     1320

cugcagaggg uuaaggauaa cuccgugauc ccauaucaga uucaccggua cgagcugaaa     1380

aagauccugg aaaaugccag uaaaaaguuu acuuuccuaa acgaggaauc agacgggcau     1440

accaacauug agaaaaucau aaagaucaug gaguaucgca uucccuacua uguaggaccu     1500

cucaaugauc accauagcgu guacaacaau ggcuuugcuu gggucucccg aaagagaucu     1560

ggcaggauca caccauggaa cuucgacgaa augguggaua aagaggaaag cgccgagcgg     1620

uuuauugaaa aucugaccaa caagugcuca uaucuuaucg gggaggacgu uuugcccaaa     1680

gagagucugc ucuacuccga guucagccug cuuaaugaac ugaacaauau aaaguacgau     1740

ggaauuucuu ugccuaucga cauuaaaaag ucaaucgugg aaaagauauu ucgcgucgag     1800

aaaaagagag ugacgaaaaa guauaucaaa gaguuccuca agcaagaagg uuacagugau     1860

ggcaaagggg agguaagcgg aauugacauc gauauuaagu ccgaccugaa gucuuauagg     1920

gauaugguua aaaucuucgg cacugaaaua gacuaccgua uggcggagaa cauuaucaag     1980

ugggugacau uauuugguga cucgaaagau cugcuaaaga ccaaaaucaa uaaggaauau     2040

ggggagaagg ucagcaaaga ccagcucaag aaagugugua agcugaaaua ccgggauugg     2100

ggccgacuuu ccaagaaguu cuugacugga auuagaucaa guguccuggu gaacgacgca     2160

acaggcgagg gguauaccau cauugaagcc augaggaaua cggauaacaa ucucaugcag     2220

cuguuacgca acgaguacga cuaccugaaa aagauagauc agaucaacau gcuuucucgg     2280

aaagacauug augaaaucag ccacgacauu uuggaugacc ucuauugcuc cccagcuguu     2340

aagagaucac uguggcaaac caucaaaaua guggaggaaa ucaagaagau uaugggacau     2400

gagccgaaaa agguauuugu cgaggugaca aggacuaaua aagccgauaa gaaaaccaca     2460

aucacucgca agaaccgacu acuggaacuc uacagcgcaa uuaaugcuga gucucgggac     2520

uggguuaacg aauuaaauuc caccgaggug aguaaguuca acagcaaaaa guuguaucuu     2580

uacuauacac agauggguaa auguauguac accggcaaga auaucgacau agaaucucug     2640

uuuacugaua uguaugacau ugaucacauc uucccccguu ccaaaacgaa ggacaacuca     2700

uuugagaauu uaguccucgu gacaagaaaa gccaacgcgg auaagaggga cguauacccu     2760

aucgauccaa agauucagac cgagcgcaaa agucuguggg acaugcuuca cgauaagaaa     2820

cugauagggg acaagaaaua cgaucgguug acuagaacca caggauucac cgacgacgaa     2880

augagcggcu uuaucgccag gcagcucgug gagacuucgc aagcauccaa ggcugccgcu     2940

gaaaugcuga agcuaauuaa uaacgauucu gagaucguuu augucaaagc cgagaaugug     3000

agcgacuucc gacaugaaaa cgaguuuauu aaggugcgcg aaaucaauga ucaccaucac     3060

gcaaaagacg ccuaccugaa cauagucguu gguaacgugu auaaugagaa guucacgaaa     3120

aaccccgcua auuacaucaa gaaagggggc ggaaacaauu auucacucaa cugcauuuuu     3180

aaucggaacc uggauagaaa uggcaaaagg aucugggacg aaaagaaaag uauugauaca     3240

gugaaccgua ugaugaaguc caaugacgua cgcgucaccu ucaaaaaccg gguggauaag     3300

gggccuaucc uuaauaaggu uacuauacac aaagcgucug aguucaugga cgauggaaag     3360

aaaaacaaga aagguuuucc aauuaagagc uccgagaaaa auggcaacaa uagauugugu     3420

aaccuguaca aguauggggg auacaaggac uuaaacuacu cacauuauac aaucgugagg     3480

uacaaugucu augagaaaaa gggccucgau aucaaauacu auaugauucc caucccguac     3540

uaucugugcc uuuguaacau aaaucagcac gacuacgugu acgacuauau uagcaaccga     3600

aguaagaaag auguuaauaa caucgaaauu aucgugaaug aacugaagau ggguucucua     3660

auaaacauug acggguacaa uuauuaccuc ggcggaucca agccuaaaac cuucuaugua     3720

gaucccgcau accagaucau ccuggacaag gagauugaac ggugcaucaa agagauuguc     3780

aaggccaaaa acaaugagua ucgcuuuaua aacacagaua agaucaauaa gaacuugaau     3840

augaaaauuu acaaguacau cgugaacaaa aagaucauug gcguguucag aaacaggaaa     3900

ugggacaagg gggaauaucu gaauagcucg gagacuauag aaaaguuuuc aggauuaucu     3960

gucgagaguc aauguaacgu uauccuccgg cugauuaaug auaugcgagg cuccaacaaa     4020

gguggggcua auagcaaguu cgacgaacuu gagaugacca ucucuagagc cgagauucca     4080

cagacgcuga gcaacguguc cucauuuaaa aucauaaauc agaguaucac cggauuguuc     4140

gaaaacgagg uaacaauugu guga                                            4164


<210>  3230
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3230
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

auaacugaug acuauaaagu uccuucuaag aaaaugaagg ugcucgguaa uacgaguaaa      120

aaguacauca agaaaaaccu ucugggcgcu uuguuauucg auucagggau uaccgcagag      180

ggaagaaggc ugaagcggac agcccgccga cguuauacua gaaggcggaa ucgcauccua      240

uaccuccagg aaauuuuuag caccgagaug gcgacacugg acgaugcuuu cuuucaaaga      300

cuugacgauu ccuuccuggu accagacgau aaaagggacu cuaaguaucc gaucuuuggc      360

aacuuggugg aagagaaagc cuaccacgau gaguucccca ccauauauca ucuccggaag      420

uaccuggcag acucgacuaa gaaagccgau cugcgccucg ucuacuuagc ucuggcacac      480

augaucaagu aucgagguca uuuucuuauu gaaggggagu ucaauagcaa aaacaaugac      540

auccagaaga acuuucagga cuucuuggau acauacaaug ccauuuuuga aucagaccug      600

agucuagaga acuccaaaca gcucgaagag aucgugaagg auaaaauauc uaagcuggag      660

aagaaagaca ggauucuuaa gcuguucccu ggagaaaaga auagcggcau cuuuuccgag      720

uucuugaaac ucauuguugg caaccaagcu gauuucaaaa aguauuuuaa ucuggacgaa      780

aaggccucau uacacuucag uaaagagagc uacgaugaag accuggagac ccuccuuggg      840

uauaucggag augacuacuc ugauguguuu cugaaggcga aaaaguugua ugacgcaauc      900

cuacuguccg guauucucac ggucacugac aacggcaccg aggccccacu gucaagcgcu      960

augauaauga gguacaaaga acaugaggaa gaucuugggc uguugaaggc cuacauccgg     1020

aauauuaguc ucgagacaua uaacgaagug uucaaggaca acucuaaaaa uggauacgca     1080

ggcuauaucg augguaagac uaaccaggag gacuuuuacg uauaucugaa aaauuuacug     1140

uccaacuucg agggggcuga uuacuuucua gaaaagauag accgugagga uuuccuccgc     1200

aaacagagaa ccuuugacaa uggaagcauu cccuaucaga uccaucugca agaaaugagg     1260

gccaucauug auaagcaggc gaaguucuac ccuuuucuug ccaacaauaa agagcggauc     1320

gaaaagauuu ugacauuccg aauaccauac uauguuggcc cccuggcacg cggcaacucg     1380

gacuuugcuu ggucuaucag aaaaaggaau gagaagauua ccccuuggaa cuucgaggau     1440

gugaucgaca aagaauccuc agccgaggcu uucaucaauc ggaugacgag uuuugaucuc     1500

uaccugccag aagagaaggu ccuuccgaaa cacagccugu uguaugagac uuucaacgug     1560

uacaaugaac ucacaaaggu gcgcuuuauu gccgagucca ugucuaagua ucaguuccug     1620

gacagcaaac agaagaaaga cauagucaga uuauacuuua aggauaaaag gaagguuacc     1680

gacaaggaua ucauugaaua ucugcacgca aucgacgggu acgauggaau ugagcuaaaa     1740

gguaucgaaa agcaauucaa cucaagucuc uccacuuacc acgaccugcu uaauauaauc     1800

aacgauaaag aguuuuugga cgauagcucu aacgaggcca uuaucgaaga gauuauccau     1860

acacugacca uauucgaaga ccgugagaug auuaagcagc ggcucucaaa auuugaaaau     1920

aucuucgaua agagugugcu gaagaaauua agccgaagac acuauacggg cugggggaag     1980

cuguccgcua aacuuauuaa cggaaucagg gacgagaagu cuggcaauac cauauuggau     2040

uaccucaucg acgacgguau uucgaaccgc aauuuuaugc agcugaucca cgaugacgcg     2100

cugagcuuca aaaagaagau ucaaaaagca cagaucauag gggaugagga caagggcaac     2160

auuaaagaag uagugaaguc ccugcccgga aguccugcca ucaaaaaggg cauccuccag     2220

ucaauuaaaa ucgucgauga gcuggugaag guuaugggag ggcggaagcc agaaucuauu     2280

guggucgaga uggcuagaga aaaucaguau acaaaccaag guaaaagcaa uucccagcag     2340

aggcuuaagc gcuuggagaa aucacugaag gaguuaggca gcaaaauacu caaggaaaac     2400

auccccgcca agcugucuaa aauugacaau aacgcacuuc agaaugaucg acuguacuug     2460

uauuaccucc aaaacgggaa ggacauguau acuggagaug accuggauau cgaccggcua     2520

uccaauuacg acauugauca uaucauaccu caggccuuuc ugaaagacaa caguaucgau     2580

aacaaggugc ucguaagcuc ugcuuccaau cguggcaaau cagacgaugu uccaagucug     2640

gaggugguca agaagagaaa aacccuuugg uaccaguugc uaaagagcaa auuaauuucg     2700

cagaggaagu ucgacaaccu cacaaaagcc gaacgcggug ggcugucccg ggaggauaag     2760

gcaucuuuca uccaaagaca gcuuguggag acuaggcaga uuaccaagca cgucgcgcga     2820

cugcuagacg aaaaauuuaa uaacaagaaa gaugagaaua accgcgcugu gcggacaguu     2880

aagaucauaa cccucaaaag cacucuggug ucacaguuca gaaaggacuu ugaauuguau     2940

aagguaaggg agauuaauga ucugcaucac gcccaugacg cauacuuaaa cgccgucgug     3000

gcuagugccc uccugaaaaa guaucccaaa cuugaaccgg aguucguuua cggcgauuau     3060

ccuaaguaca auuccuuucg ugagcgcaaa ucugcaacgg aaaaggugua uuucuacagc     3120

aacaucauga auaucuuuaa aaaguccauu ucacuggcug acggaacagu cauagagaag     3180

cccuugaucg aagugaacga ggaaaccggc gagagcguau ggaauaaaga gagugaccuc     3240

gccacugugc ggagaguucu gucuuaccca caagucaacg uggugaagaa agucgaaaag     3300

cagaaucacg ggcuagauag gggaaaaccu aagggucugu ucaacgcgaa ccuuuccagc     3360

aagcccaaac caaauucgaa cgagaaucuc guuggcgcca aggaauaucu ggaccccaaa     3420

aaguacgggg gauaugcugg cauuucaaac ucuuuugcag uguuggugaa agguaccauc     3480

gagaaggggg ccaagaaaaa gauuacaaau guacuggagu uccagggcau caguauauua     3540

gaucgaauca acuaccggaa agacaagcuc aauuuucugc uugaaaaagg auauaaggau     3600

auugagcuga ucauugaauu gccuaaguac ucccucuucg agcugagcga cggcucucgc     3660

agaaugcuag cuagcauccu guccaccaac aauaaaagag gggaaauaca caagggaaac     3720

caaauuuucc uuucacagaa auuugucaag cuccuguauc augccaaacg gaucaguaau     3780

acuaucaacg agaaucaccg aaaguacgug gagaaccaua aaaaggaguu cgaagaauug     3840

uuuuacuaua uuuuagaguu caaugaaaac uacguuggcg caaagaaaaa ugguaagcug     3900

cucaacagcg cguuucaguc uuggcagaac cacuccaucg augagcugug cucaaguuuc     3960

auagggccaa caggaagcga gagaaaaggc cuuuuugaac ugacgucccg cgguucugcc     4020

gcugacuucg aguuuuuggg cgugaagauu ccgagguauc gugauuacac ccccucgagc     4080

cuacugaaag acgccacucu cauccaccaa ucagucacag ggcuguauga aacccggauu     4140

gaucuugcaa aguuaggaga gggcuga                                         4167


<210>  3231
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3231
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

auaacugaua auuauaaagu uccuucuaag aaaaugaagg ugcucgguaa cacgaguaaa      120

aaguacauca agaaaaaucu ucugggcgua uuguuauucg acucagggau uaccgcugag      180

ggaagaaggc ugaagcggac agcacgccga cguuauacua gaaggcggaa ccgcauccua      240

uaccuccagg aaauuuuuag caccgagaug gccacacugg augacgcguu cuuucaaaga      300

cuugaugacu ccuuccuggu gccagaugac aaaagggauu cuaaguaucc gaucuuuggc      360

aauuuggucg aagagaaagu guaccacgac gaguucccca ccauauauca ucuccggaag      420

uaccuggcug auucgacuaa gaaagccgac cugcgccucg uuuacuuagc acuggcccac      480

augaucaagu aucgagguca uuuucuuauu gagggggagu ucaacagcaa aaauaacgau      540

auccagaaga auuuucagga cuucuuggac acauacaacg cuauuuuuga aucagaucug      600

agucuagaaa auuccaaaca gcucgaagag aucgugaagg acaaaauauc uaagcuggag      660

aagaaagaua gaauuuuaaa gcuguucccu ggagaaaaga acagcggcau cuuuuccgag      720

uucuugaagc ucauugucgg caaucaagca gacuucagga aaugcuuuaa ccuggaugaa      780

aaggccucau uacacuucag uaaggagagc uaugacgaag aucuggagac ccuccuuggg      840

uacaucggag acgauuauuc ugacguguuu cugaaagcua agaaauugua cgaugccauc      900

cuacuguccg guuuccucac gguaacugac aaugagaccg aagcgccacu gucaagcgca      960

augauuaagc gguauaacga gcauaaagaa gaccuugccc uguugaagga guacauacgu     1020

aacaucaguc ucaagacaua caaugaaguu uuuaaagaug acacuaagaa cggcuaugcu     1080

ggguacauug auggaaaaac caaucaggag gacuucuaug uguaccugaa gaacuuacug     1140

gccgaguuug aaggcgcaga uuauuuccua gagaaaaucg accgcgaaga uuuucucaga     1200

aagcagagga cauucgacaa ugguucuaua cccuaccaga uucaucugca agagaugcgg     1260

gcuauccuug auaagcaggc caaauuuuau ccuuucuugg cgaagaacaa agaacgaauc     1320

gagaagauuc ugaccuuucg caucccauac uacgucgggc cccucgccag aggaaauucc     1380

gacuucgcau ggagcauuag gaaacggaac gagaagauaa cgccuuggaa uuucgaagau     1440

gugaucgaca aagagucguc ugcugaagcc uuuauuaacc gcaugacuuc cuucgaucug     1500

uaucuuccag aggagaaggu gcugccgaag cacucauugc ucuacgaaac auuuaauguc     1560

uauaacgagc ugaccaaagu uagauucauc gcugaaagua ugagggacua ccaguuuuua     1620

gacagcaagc agaaaaagga uaucgugcgu cuguauuuca aagacaagcg gaagguaacu     1680

gauaaagaca uuauagagua ccuacacgcc aucuauggcu acgauggcau ugaacucaag     1740

gggaucgaga aacaauuuaa uuccucucug agcacauacc acgaccuuuu gaacauuauc     1800

aaugauaagg aguuccugga cgauucaagu aacgaagcaa uaaucgagga aauuauccau     1860

acccucacga uuuuugagga ccgagaaaug aucaaacaga gacuguccaa guucgagaac     1920

auauuugaua agagcgugcu uaaaaagcug ucuaggcgcc acuauaccgg augggguaaa     1980

cuuucagcca aguugauuaa uggcauccgg gacgagaaaa gugggaacac aauucucgau     2040

uaccugaucg acgacggaau aagcaauaga aacuucaugc agcuaaucca cgaugacgcu     2100

cuguccuuua agaagaaaau ucaaaaggcg cagaucauug gcgaugaaga caaagguaau     2160

aucaaggagg ucgugaaauc ucuccccggg ucgccugcaa uaaagaaagg cauucugcag     2220

agcaucaaga ucguugauga acuugugaag gucaugggag gcaggaaacc agaguccauu     2280

gugguagaaa uggcccgcga gaaccaguau acuaaucaag ggaagucaaa cagucagcag     2340

cgauugaaac ggcuggagaa gucuuuaaaa gaacucggaa gcaagauccu gaaggagaau     2400

auucccgcua aacuuuccaa gauagacaac aaugcccugc agaacgaucg uuuguaccuc     2460

uauuaccugc aaaaugguaa agacauguau accggcgaug accuagauau cgacagacug     2520

ucaaacuacg acauugauca uaucauuccu caggcauucc ucaaggacaa uagcaucgau     2580

aacaaaguuc uggugucuuc cgccaguaac aggggaaaga gcgacgauuu cccaucucuu     2640

gaagucguga agaaacgcaa gacauuuugg uaccaguugc ugaaauccaa guuaauauca     2700

cagcggaaau ucgacaaucu cacuaaggcu gagagagggg gccugcuucc cgaggauaag     2760

gccgguuuua uccaaaggca gcuggucgaa acccgacaga uuacaaaaca cguggcacgc     2820

cuacucgacg agaaguucaa caauaaaaag gaugaaaaca aucgggcggu uagaaccgug     2880

aaaaucauua cucugaagag uacguuggua agccaguuua ggaaggacuu cgagcuguau     2940

aaaguccgug aaaucaacga uuuucaucac gcucaugacg ccuacuuaaa ugcagugaua     3000

gccucggcuc uccugaagaa auauccgaaa cuugagccug aguucguuua cggggauuau     3060

cccaaauaca acuccuuucg cgaacggaag ucugccacag agaaagugua uuucuacagc     3120

aauauuauga acaucuuuaa gaagucaauc agucuggcag acggcagagu cauugaaagg     3180

ccauugauag aggugaauga agagaccgga gaguccguau ggaacaaaga aucugaccuc     3240

gcuacugugc gacggguucu gagcuacccu caagucaaug uggugaagaa agucgaggaa     3300

cagaaccacg gccuagaucg cgggaagccc aaaggacugu ucaaugccaa ccuuuccuca     3360

aagccaaagc ccaacagcaa ugagaaccuc guuggugcga aagaauaucu ggacccuaag     3420

aaauacggcg gguaugccgg aaucaguaau ucuuucgcug uguuggugaa gggcaccauu     3480

gagaaaggug caaagaagaa aaucacaaac guacuggagu uucaggggau uuccaucuua     3540

gauagaauaa auuacaggaa ggacaaacuc aacuuccugc uugaaaaggg cuauaaagau     3600

aucgagcuga uuaucgaauu gccaaaguac agccucuuug agcugucgga cggaucacgg     3660

cgaaugcuag ccucuauucu gaguaccaau aacaagagag gcgagaucca caaagggaau     3720

caaauauucc uuucccagaa guuugucaaa cuccuguauc augcuaagcg cauuagcaac     3780

acuaucaaug aaaaccacag gaaauacgug gagaaucaua agaaagaguu cgaagaauug     3840

uuuuacuaua ucuuagaguu caacgagaau uacguuggag ccaagaagaa cggcaaacug     3900

cucaauucug cauuucagag cuggcagaac cacuccauug augaacugug uucaaguuuc     3960

aucgguccga cagggagcga gcguaaggga cuuuuugaac ugacgucucg gggcuccgcg     4020

gccgacuucg aguuuuuggg ugugaaaaua cccagauaua gggauuacac cccuucaagu     4080

cuacugaagg acgcuacucu cauucaccaa agcgucacag gccuguauga aacccgcauc     4140

gaucuugcca aauuagggga gggauga                                         4167


<210>  3232
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3232
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

auaacugaua auuauaaagu uccuucuaag aaaaugaagg ugcucgguaa cacgaguaaa      120

aaguacauca agaaaaaucu ucugggcgua uuguuauucg acucagggau uaccgcugag      180

ggaagaaggc ugaagcggac agcacgccga cguuauacua gaaggcggaa ccgcauccua      240

uaccuccagg aaauuuuuag caccgagaug gccacacugg augacgcguu cuuucaaaga      300

cuugaugacu ccuuccuggu gccagaugac aaaagggauu cuaaguaucc gaucuuuggc      360

aauuuggucg aagagaaggc uuaccacgac gaguucccca ccauauauca ucuccggaag      420

uaccuggccg auucgacuaa gaaagcagac cugcgccucg uguacuuagc ccuggcucac      480

augaucaagu aucgagguca uuuucuuauu gagggggagu ucaacagcaa aaauaacgau      540

auccagaaga auuuucagga cuucuuggac acauacaacg caauuuuuga aucagaucug      600

agucuagaaa auuccaaaca gcucgaagag aucguuaagg acaaaauauc uaagcuggag      660

aagaaagaua ggauucuuaa gcuguucccu ggagaaaaga acagcggcau cuuuuccgag      720

uucuugaaac ucauuguggg caaucaagcc gacuucagaa aaugcuuuaa ccuggaugaa      780

aaggcuucau uacacuucag uaaggagagc uaugacgaag aucuggagac ccuccuuggg      840

uacaucggag acgauuauuc ugacgucuuu aucaaagcca agaaacugua cgaugcgauu      900

uugcuauccg guuuccugac ggugacugac aaugagaccg aagcaccacu cucaagcgcc      960

augauaaagc gguauaacga gcauaaagaa gaccuggccc uucugaagga guacauccgu     1020

aacauuaguu ugaagacaua caaugaagua uuuaaagaug acacuaagaa cggcuaugcu     1080

ggguacaucg auggaaaaac caaucaggag gacuucuaug uuuaccucaa gaaccuguua     1140

gcagaguuug aaggcgcuga uuauuuccug gagaaaauag accgcgaaga uuuucuaaga     1200

aagcagagga cauucgacaa ugguucuauu cccuaccaga uccaccucca agagaugcgg     1260

gccauccugg auaagcaggc gaaauuuuau ccuuuccuug ccaagaacaa agaacgaauu     1320

gagaagaucu ugaccuuucg cauuccauac uacguggggc cccuggcaag aggaaauucc     1380

gacuucgcuu ggagcauaag gaaacggaac gagaagauca cgccuuggaa uuucgaagau     1440

gucauugaca aagagucguc ugccgaggcu uuuaucaacc gcaugacuuc cuucgaucuc     1500

uaucugccag aagagaaggu gcuuccgaag cacucacugu uguacgaaac auuuaaugug     1560

uauaacgagc ucaccaaagu cagauucauc gccgaaagua ugagggacua ccaguuucug     1620

gacagcaagc agaaaaagga uauuguucgu uuauauuuca aagacaagcg gaaggugacu     1680

gauaaagaca uaaucgagua ccugcaugca auuuauggcu acgauggcau cgaacuaaag     1740

gggauugaga aacaauuuaa uuccucucuc agcacauacc acgaccugcu uaacaucaua     1800

aaugauaagg aguucuugga cgauucaagu aacgaagcca ucauugagga aaucauucau     1860

acccugacga ucuuugagga ccgagaaaug auaaaacaga gacucuccaa guucgagaac     1920

auuuuugaua agagcguacu gaaaaaguua ucuaggcgcc acuauaccgg augggguaaa     1980

cugucagcua aacuuaucaa uggcauucgg gacgagaaaa gugggaacac aaucuuggau     2040

uaccucauag acgacggaau cagcaauaga aacuucaugc agcugauuca cgaugacgcg     2100

cuauccuuua agaagaaaau ccaaaaggca cagauuaucg gcgaugaaga caaagguaau     2160

auaaaggagg uggucaaauc ucugcccggg ucgccugcca uuaagaaagg cauccuccag     2220

agcaucaaga uuguggauga acugguuaag gugaugggag gcaggaaacc agaguccauc     2280

gucguggaaa uggcucgcga gaaccaguau acuaaucaag ggaagucaaa cagucagcag     2340

cgacuuaaac gguuggagaa gucucugaaa gaauuaggaa gcaagauucu caaggagaau     2400

auacccgcca aacuguccaa gaucgacaac aaugcacuuc agaacgaucg ucuguacuug     2460

uauuaccucc aaaaugguaa agacauguau accggcgaug accuggauau ugacagacua     2520

ucaaacuacg acaucgauca uauuaucccu caggccuucc ugaaggacaa uagcauagau     2580

aacaaaguac ucguuucuuc cgcuaguaac aggggaaaga gcgacgauuu cccaucucug     2640

gaagugguca agaaacgcaa gacauuuugg uaccagcuuu ugaaauccaa gcugaucuca     2700

cagcggaaau ucgacaauuu aacuaaggcc gagagagggg gccuccugcc cgaggauaag     2760

gcagguuuua uucaaaggca gcuuguggaa acccgacaga ucacaaaaca cgucgcgcgc     2820

cugcuagacg agaaguucaa caauaaaaag gaugaaaaca aucgggcugu gagaaccguu     2880

aaaauuauca cucucaagag uacgcuggug agccaguuua ggaaggacuu cgaguuguau     2940

aaaguacgug aaauaaacga uuuucaucac gcccaugacg cauaccugaa ugccgucauu     3000

gcuucggccu uacucaagaa auauccgaag cuggagccug aguucgugua cggggauuau     3060

cccaaauaca acuccuuucg cgaacggaag ucugcaacag agaaaguuua uuucuacagc     3120

aauaucauga acaucuuuaa gaagucaauu agucuugcug acggcagagu gauagaaagg     3180

ccacugaucg aggucaauga agagaccgga gaguccgugu ggaacaaaga aucugacuug     3240

gccacuguac gacgggugcu cagcuacccu caaguuaaug ucgugaagaa aguggaggaa     3300

cagaaccacg gccuggaucg cgggaagccc aaaggacuau ucaaugcgaa ccuguccuca     3360

aagccaaagc ccaacagcaa ugagaaccuu gucggugcca aagaauaucu cgacccuaag     3420

aaauacggcg gguaugcugg aauuaguaau ucuuucgcag uucuggugaa gggcaccauc     3480

gagaaaggug ccaagaagaa aauuacaaac guguuggagu uucaggggau cuccauacug     3540

gauagaauca auuacaggaa ggacaaauua aacuuccucc uggaaaaggg cuauaaagau     3600

auugagcuua ucauugaacu gccaaaguac agcuuguuug agcucucgga cggaucacgg     3660

cgaaugcugg cuucuauccu aaguaccaau aacaagagag gcgagauaca caaagggaau     3720

caaauuuucc ugucccagaa guuuguaaaa cuucucuauc augccaagcg caucagcaac     3780

acuaucaaug aaaaccacag gaaauacguc gagaaucaua agaaagaguu cgaagaacug     3840

uuuuacuaua uuuuggaguu caacgagaau uacgugggag caaagaagaa cggcaaauua     3900

cucaauucug cguuucagag cuggcagaac cacuccaucg augaacugug uucaaguuuc     3960

auagguccga cagggagcga gcguaaggga cuguuugaac uuacgucucg gggcuccgcc     4020

gcugacuucg aguuucuggg uguuaaaauu caaagauaua gggauuacac ccccucaagu     4080

uugcuaaagg acgccacucu gauccaccag agcgugacag gccucuauga aacccgcauu     4140

gaucuggcaa aacuugggga gggauga                                         4167


<210>  3233
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3233
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acgagaaaga aauauccaag aucauuaaau ugggugucag gguuaauccc      120

uuaacaaucg augagaagac uaacuucgaa aaaggccggc cucugucuac gaaugcagac      180

cgcacccuaa agcgaggggc ccguagaaac cuccagaggu acaagcugcg gcgcgagaau      240

cuuauugaga uccugauaga uaaccacauc auuacaaaag aaacuccauu gaccgagauc      300

ggaaagggca caaccuauca aacucucuca cugagagcga aagcugugag ggaacggauu      360

gaccuggagg auuuugccaa gguacucuua gcaaucaaua aaaagcgcgg uuacaagagu      420

agccgaaaag ccuccaacga cgaggaaggg caggcuauag auggaauggc aauugccaag      480

gagcuguaug aaagaggcau cacaccgggc caguacgugc uugaguugcu ggaaaaugac      540

aggaaauaua uucccgauuu cuaccggucu gaccuaaagc aggaguuuaa caaaaucugg      600

aauaaccaaa agaccuucua uccugagauc cucacggaug aacuguuuaa agagcuugac      660

gggaagaaua agucgcagac uugggcuauu ugccagaaac cauucaacau aaagggaauc      720

aaacguauua ccaagggucg cgaaaagaca aucgagaacu accauuggag agucaagggc      780

cugucaggga aguugaaucu cgaaaaacug gccauaauuu uacaggagau caacaaugau      840

aucaaccaaa gcaguggaua ccugggcggu auuuccgaca agucuaaaaa gcucuaucuu      900

aauaaagaga cuguggggga auuucuguac aagcagaucg cgaagaaccc ccacaccagc      960

uugaaaaauc agguuuucua uaggcaugac uaccuagaug aguuugaaca gauuuggaag     1020

acacaaucca acuucaaugc aaaccugacc gacgagcuca aacaggaaau acgggaugug     1080

aucaucuuuu aucagcgaaa gauuaaauca caaaagaguc ugaucagcuu cugucaguuu     1140

gagucuuggg agcaggaaua cuucgacaag gagacgggaa aaacuaagac acgcaccguc     1200

ggcagaaggg ugauuccuaa auccucacca cuuuuccagg aauuuaagau auggcaaaau     1260

cugaaccacu ugguauucac uaauagcgag acagaugaga aaaucgaaau uaaggagguu     1320

gaacaggaga uccggaaaga acucuuugag gagcugaacu uacgcggcga ccugaauccc     1380

aacgaucuac ucaagauucu gagaagucag uucaagaucg ggaaacuuuc uaacuggaag     1440

ugcaauuuug agaaaauaga aggaaacagg accaaugccg cuuuguucaa caucuaucag     1500

aagauuuccg acuuugaagg uuacggcauc gauuggaaua aaagcaacgc caagaagauu     1560

cguaaagagc ugaaggcaac guucccugaa aucgggauag acgcuucgau ucucgaguuu     1620

gaugccuaca aaaagcaacu gaccaaucag ucuuccuaug agcuuuggca ucuguuguac     1680

ucagcggaag aggacuucaa aaucagugaa caggacaagc ucauuuaugg aaacagcucc     1740

gugucucuga agaaaaaguu acaccugaaa uuuggcuuca auccagagua cgccaagaug     1800

cuagcaaacc ucagccugau gucagauuau gguaaucuua gugcuaaagc caucaagaag     1860

auauugccgu accuggaaga cgggcaccuc uauuccgagg cuugucggcu ggccggcuac     1920

aaccauagca aaucuuuaac agcagaggaa cuggagaagu uugaacccaa agccaaacuu     1980

gauuugcuca aaaagaacuc acugcgaaau ccugucgugg agaaaauccu aaaucagaug     2040

gugaacgucg uuaaccaagu gauugacacu uacggaaagc cagauggcgu aagaaucgag     2100

cuggcuaggg aacucaagaa aagugcgaag gagcgcgcag aaaugaccaa agggauugcc     2160

gacggaacac ggagaaauga ggaaaucaag aaaauaauua cucagaaguu cgguaucccc     2220

aacccuacca agagcgaucu ggugagguau cgccuuuggc aggaguugga gucccgaggc     2280

uacaaagaca ucuucacaga uaaguauauu ucuccagaca aaaucuuuuc gagcgaaauu     2340

gauauagagc acaucauucc caaggcucug uuauucgacg auuccuuuuc aaauaaaacc     2400

cucaguuucc ggaacgucaa ucugaagaag gccaaccgua cugcacuuga cuuuaucacg     2460

caggacuacc aaucuaaugu ggaagccuau aaagagagag uugaaacaug gaacaaggau     2520

aaucauauua gcaaagggaa gaggaacaaa cuguugaugu cccucaagga caucccggau     2580

ggauucauag agcgcgaccu gagaaauuca caguacaucg cuagaaaggc caaaaggaug     2640

cuauaugagg uguuuccuaa cgaaguccug gcaaccagcg gcucuauuac ugauaagcuc     2700

cgauccgacu gggaucugau caaugugaug aaagagcuua acuugcccaa guacaaagcg     2760

cuggguuuaa ccgaauacga ggaacgccuc gacaagggga cagaggagcu guucaagcac     2820

gaaguaauua aagauuggac caagcggaau gaccacagac aucacgcuau ggaugcccuu     2880

gcaguugccu uuacuacaca uaaccacauc caguauauaa acaaucugaa cgcuaggaaa     2940

gacauuacgc aagauaauca uccaaucauc aagaacauug agaaucuaau aaccgaagug     3000

aacaguaaag gcaagcgugu cuucaaagcc ccuaugagcu cuuuucgcga guccgcaaag     3060

gagcagaucg aagcuauucu cgugucauuc aagaauaaaa acaaggucgu gacuaaaaau     3120

aucaaccaga caaagaaaga gggaggcuuu aauuaccaga acgccaagac accccggggg     3180

caacugcaca aggaaaccau uuauggaaaa aguaagcagc cauuggagaa acccguuaag     3240

aucaauaaca gauucugcaa aaagcaagug ggucugauaa ucaauuucaa ggaacaggag     3300

gcgguauuac accaucucaa aaaguacgac aacaaugaga aaauugccuu uagcaacaag     3360

acucugaaau cggacccuau ccaggucaac ggcuccccac uuagggaagu gaagauuuuc     3420

gaggaaaucu uuaccauacg aaaggauauu aauccggaca acuucaaaac gcagaagcac     3480

cuggauaaaa ucaucgacaa ggggauuaaa gagguuauga agaaacgguu ggaagaguau     3540

aauggagauu uuaagaccgc uuucucugac cucgauaaga accccaucug gcugaaucag     3600

agcaaaggca uaacaauuaa gcgcgcaacu aucucaggug ugaccaacgc cgagccucua     3660

caucaggcua aagaccaccu ggggaauauu auccuugaug acaacggcaa ggaaauacca     3720

auugauuacg ucaguacagg aaauaaccac cauguggcca ucuaucaaga cgaguccggc     3780

aaucuccagg aagagguagu gucuuuuuac gaagcaguua uccggaaaaa ccaggaccaa     3840

cccauuauca agaagaggca cccuaaaggg uggaaguucc uguuuacuau gaaacagaau     3900

gagauguucg ucuuuagcuc cgagaacuuc aauccaucag aaauugauuu gcuggacgag     3960

aagaacgcgg ccauaaucag ccccaacuua uuccgggugc agaaaauugc uaccaagaau     4020

uacauguuuc gacaucaccu cgaaacggag gugacaaaca aucuggauuu caccuauaga     4080

cauuuucagg ccacugagug gcuucgcaac cuguguaagg ucaggaucga ccacaucgga     4140

cguauuguuc aaauaggcga auacuga                                         4167


<210>  3234
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3234
augaucauua gaaccuacga gcugaagaca auaacuacga ucgccauucu caaaauguuc       60

cagcuucuga ccggcggguu gagcaugaca aaguuaggaa aacccuaugg uaucggcgug      120

gacauugggu ccaacucuau cggauuugcu gcagucgaug aaaauucaca ccugauaagg      180

cuaaagggca aaacuguuau cggugccaga cucuucgagg aagggaaggc ggcugccgac      240

cgccgagcaa gucguaccac aagaaggcgg cugagccgca acagauggag gcuuuccuuu      300

cugcgggauu ucuuugaguc ucauauuacc ccuacuggac caaauuucuu uaugcgccaa      360

aaguacucgg aaaucucacc gaaagacaag gcccgauaua aauacgagaa gagauuguuc      420

aacgauagga cagacgcuga guuuuaucag caguacccca ccauguauca ccuccggaau      480

cgucugcuga cggauccuag caaagcagac gugcgcgaaa uuuacuucgc cauccaucac      540

auacucaaga guagaggcca uuucuuaacu uccggcuacg cuaaggauuu caacaccaau      600

aaaguagccc ugaacgagau uuuuccagcg cuucaggacg cauaugccca aguguacccc      660

gauuuggaca ucacauucga ugaaaauaag gucaacgagu ucaaaacugu gcugcuaaau      720

gagaaggcua ccccuucuga cacacagagg gcccucguua accugcuucu ggcagaagac      780

ggggauaaag acauuuugaa gcagcagaaa caagugcuca ccgaguuugc uaaggccguc      840

gugggacuga agacgaaauu aaauguagcg cuggguacug aaguugauag cuccgaggcc      900

acagugugga acuucucacu cggccagcuu gacgauaagu gggcagggau cgaaagugcu      960

augaccgacg agggaacuga gauccuggau cagauucggg acuuguaucg agcccgccua     1020

cugaauggca uagucccagc ugguaaaaca cucagccagg ccaaggugga ugacuacgca     1080

caacaccacg aagaucugga gcuuuuuaaa gacuaucuga agcaguugga agacgauggg     1140

accgccaagg cuaucagaca gcucuacgac agguauauug auggagacga ugcgaaaccc     1200

uucgugcggg aggacuuugu caaggcacug acgaaagaag uuaccgccca uccuaacgcu     1260

aagucuccag aguuacugga gcgccuagcc caaccggauu ucaugcucaa acagagaaac     1320

aaggcaaaug gcgccauccc cgugcagaug cagcaaaggg aacuggacca gauaauuaag     1380

aaccaguccg uauacuacga uuggcuugcu gccccuaauc caguggagaa acaccguaag     1440

ucaaugcccu aucaguugga cgaacugcuc aacuuucgga ucccuuacua ugucggccca     1500

cuggugacag caaaagagca aaaggcggcu caagggggag uuuucgccug gauggugcga     1560

aaagaucccg acgguaauau cacuccguac aacuuugaug aaaaggucga cagagaggca     1620

agcgccaaua ccuucauuca gaggaugaag acaacugaca ccuaucuuau cggcgaggau     1680

gugcugccua aacagaguuu gcucuaccag cgcuaugaag uacugaacga guuaaauaac     1740

guucggguga auaacgaaaa gcugucuauu gagcagaaac agcaggucau aagagagcua     1800

uuugaaaggc auaauuccgu gacaaucaag caauucgcug agaaccuccg cgcccacggg     1860

gacuacgcac acauucccga aauccgagga cuggcugaug agaaacgguu ucuuagcucg     1920

uugucuaccu accgucagcu gaagucccuc cugccagacg ccaucgauga cccugcgaaa     1980

caggccgaua uugaaaauau aaucgcuugg ucaacugucu ucgaggacgc agccauuuuc     2040

aagacgaagu uaaaagagau caacuggcug gauagucaaa caauuaccaa acuuagcaac     2100

aucagauaua ggggcugggg ucaguuuucc cauaaauucu ugaaugggcu cacucugggc     2160

aacggacaca ccauaaucca ggaacuauuu cugucuaaua acaaucucau gcagauucug     2220

acagacgaga cccuucaaaa gaaaaugacu gaauugaacg cugauaagcu gaaggccgca     2280

aauaucaacg acgcgauuga ugacgccuac acaagccccu caaauaaaaa ggcuuuacgc     2340

caggugcucc ggguuaucga ggacauaaaa agagccgcag auggccagga cccaaguugg     2400

cuguaugugg aaacggcuga ugggggaggu acccccggca agaggacucg agcccgccag     2460

caucaacuuc aggagauuua cgcaaacgcc gcucacgagc ugaucgacuc cgcgguaaga     2520

ggggaauugg aggauaaaau ugccgacaag gcagauuuca augaccggcu cguccuguau     2580

uuuaugcagg gaggcaggga uaucuacaca gguaccccuc uaaacauaga ccagcugagc     2640

ucuuaugaua ucgaccauau ucucccacaa ucacugauca aggacaauag ucuugauaac     2700

cguguguugg uucacgcuau uaucaaucgc gaaaagaacg ccacauucgc cagcacuaua     2760

uacgcucaga agaugaacgc aaccuggcgg caguggcacg aggccgggcu gauuuccggc     2820

agaaaauuaa ggaaucucca aaugcgaccg gaccagaucg auaaguacgc gucuggauuu     2880

guggcucggc agcuggucga aacgcgccag aucauuaaac uuaccgagca aaucguggcc     2940

gcacaguauc ccgagacaaa gauuauagcc guaaaggcug gccugucgca ucagcuaaga     3000

gaggaacucg aauuuccuaa aaacagggac gugaaucacu accaucacgc auuugaugcc     3060

uuccuggcgg cucggaucgg gacuuauuug cugaagcgau uuccaaacuu acaggccuuc     3120

uuuaccuacg gaaaauucaa gaaagcagac guuaagaaac ucagagguuu caauuuuauu     3180

cgcgauauca cacacgccga ggacaagauu gucgugaagg auacuggcga ggugaucugg     3240

gacaaaaaga gggaugucga cgaacuggau cguauauaua acuucaaacg gaugcuuauc     3300

acccaugagg uucacuuuga aaaggcugac cuguucaaac aaacggugua cggggccaag     3360

gacagcaagg aggcaggagg caaaaagcag uugauuucca aaaagaaagg uuaucccgug     3420

gauaucuacg ggggcuacac acaggaaacc ggaucauauc ucaguguagu cagacugacu     3480

aagaaggcua uguacgccgu gguuaaagug ucuacaaggg acgcggccaa gcuagcaguc     3540

gcuaaaagca uuuccgagca gaaggagaau gaaacccuga acgauaucau agacgagaaa     3600

cuuucaaaga uuagcaagaa uggcaaaauc acucaugaac ucuuugaggu gguucugccu     3660

cgcguggggc aaaagaccuu guucaaaaac ucuaaguaua aucuguuuuu aguaaacucc     3720

gauacauaca ugcacaauua ucaggaacuc uggaugccac gagaguacca gaaaaugugg     3780

aaggacauca gucugagcaa guauggagau gcccagacgg aggaccaacu ugaucagauu     3840

uucggcuuua uagucucuca ggugaacucc uacuucaauc uguacgacau caaccaauuu     3900

cggaaaaagu ugaacgaugc ugcagacaaa uucgccaccc uccccaugcg ggauacugac     3960

gacaugcagg guaagauugc cacaaucggg cagcugcuaa uuggacugca ggcuaaugcg     4020

gcaaggucag aucuucgcaa ccucgacauc aaaaccccgc ugggcuuguu acaaauaccu     4080

aaugguauca gucuggauag cgacacaucg guggucuauc aguccccaac uggccuccug     4140

gaacgugagg uucaucuuuc ugaucuguga                                      4170


<210>  3235
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3235
auggccagag acuacagcgu gggccuggau aucgggaccu ccucugucgg augggcugca       60

auugacaaca aguaucaccu cauaagggcc aaaucaaaga aucuuaucgg uguucggcug      120

uucgauagug cggugacagc ugagaaacgc cgaggcuacc guacuacgag aaggcgguug      180

agccgcagac auuggagguu acggcugcua aacgacauuu uugccgggcc ccucaccgau      240

uucggagacg aaaauuuucu ggcacgccuu aaguauuccu ggguacaccc ucaggaucaa      300

ucuaaccagg cccauuucgc ugcaggccug uuguuugacu cgaaagagca ggauaaggac      360

uucuaccgaa aguauccaac aaucuaccac cucagacugg cccugaugaa ugaugaccag      420

aaacaugauc ucagggaagu guauuuagcu auucaccacc uggucaagua ccggggucau      480

uuucuuaucg agggggacgu gaaagccgac ucagcguucg auguucacac uuuugcagac      540

gccauacaac guuacgcuga aagcaacaau agugaugaga acuugcuggg aaagaucgac      600

gagaaaaagc uauccgccgc acucaccgau aagcauggcu cuaaaagcca gcgcgcugaa      660

acagccgaga ccgcguucga cauucuggau cuucagucca agaaacagau ccaagccauu      720

cugaagucag uggucggcaa ucaggcaaac uugauggcua ucuuugggcu cgacaguagc      780

gccauaucua aagaugaaca gaagaauuau aaauucuccu uugacgaugc ugacauugac      840

gagaagaucg ccgauucaga ggcacuguua agcgacacug aguucgaguu ccugugcgau      900

cucaaggccg cuuuugacgg acuuacacug aaaauguugc uaggugauga caagaccgug      960

agugcggcaa ugguaagaag guucaacgaa caccagaaag auugggagua cauuaagucu     1020

cacauccgga augccaaaaa cgcuggcaau gggcuguaug aaaaguccaa gaaauuugac     1080

ggaaucaacg ccgcauaccu cgcccugcaa agcgauaacg aggacgaucg aaagaaagcu     1140

aagaaaauuu uccaggacga gauaucgucu gccgauaucc cggacgacgu uaaggcagau     1200

uuucuuaaga aaauugacga ugaccaguuc cugcccaucc aacgcacgaa gaauaacggc     1260

acuauaccuc aucaguugca cagaaaugaa cucgagcaga uuaucgaaaa acaggguauc     1320

uauuacccau uucugaagga uaccuaucaa gagaacuccc augaauuaaa uaaaauuaca     1380

gcgcugauca acuucagggu gcccuacuac gucgggccuc uaguggagga ggaacagaag     1440

auugcugacg auggaaagaa uauaccagac cccacuaacc acuggauggu gcggaaauca     1500

aaugauacca ucacaccuug gaaccucagu caggucguug accuggauaa gagcggccgc     1560

agauuuauug agaggcuuac cggcacggac acuuauuuga ucggggaacc aacacugccg     1620

aaaaauuccc uccuguacca gaaguucgau gugcuucaag agcugaacaa uauccgugua     1680

ucuggacggc gauuggacau uagagccaaa caggacgcau uugaacaucu cuucaaggug     1740

cagaaaaccg ucagcgccac uaaccugaag gauuuucuug ugcaggcugg uuauauauca     1800

gaggacacac aaaucgaggg ccuggccgau guuaauggga agaacuucaa caaugcacua     1860

accacguaca acuaucucgu gaguguccug ggaagggagu ucguggagaa ucccuccaac     1920

gaagaacuuu uggaggaaau uaccgagcug cagacaguau uugaagacaa aaagguucuc     1980

cgccggcagc uggaucaguu agacggccug agcgaucaca auagagagaa acuuucuagg     2040

aagcacuaca cugguugggg gcgcaucagu aaaaaguugc ucaccacaaa gauugugcaa     2100

aacgcugaca aaaucgauaa ucagacuuuc gacgucccuc gaaugaacca gucaauaauc     2160

gauacccugu auaauacaaa gaugaaccua auggaaauua ucaauaacgc cgaggacgac     2220

uuuggcgugc gggcguggau ugauaaacaa aauaccacug acggagauga gcaggacguc     2280

uacagccuga ucgaugaacu cgccggccca aaggagauaa aacgugggau ugugcagucc     2340

uucagaaucc uggacgauau uacgaaggcu guuggauacg cacccaagag gguguaucuu     2400

gaauuugccc gcaaaacaca ggagucucau uugaccaacu cgcggaagaa ucaacugagc     2460

acuuuacuca aaaacgcugg ucuguccgaa cuuguaaccc aggucucaca guacgacgcc     2520

gcagcgcugc agaacgauag auuguaucuc uacuuccugc aacagggcaa ggacauguau     2580

aguggggaga aacuaaaucu ggauaaccuc ucuaauuacg acaucgauca cauaaucccu     2640

caggccuaua caaaggacaa cagccuggac aauagggugc uuguuuccaa cauuaccaau     2700

cgacgcaagu cagauagcuc uaacuacuug ccagcucuga ucgacaaaau gcggcccuuu     2760

ugguccgugu uaaguaagca gggacuccug agcaaacaua aguucgccaa ucuuacuaga     2820

acaagggauu uugacgauau ggagaaagaa cguuucauug cacgcucucu ggucgagacg     2880

cggcaaauca uaaagaacgu ggcuucccua auugacucac acuuuggcgg ugaaaccaaa     2940

gccguagcaa ucagaaguag ccucacugcc gauaugaggc gauacgugga caucccgaag     3000

aaucgggaua uuaacgacua ucaccaugcu uucgaugcgc uguuguuuuc gacaguuggg     3060

caguacaccg aaaauuccgg ccugaugaag aaaggacagu uaucugacag cgccggcaac     3120

caauauaauc gcuacaucaa ggaauggauu cacgcagcua gacucaacgc ccagucacag     3180

agggucaacc cuuucggguu cgugguggga aguaugcgga augccgcucc cgguaaacug     3240

aacccagaga caggcgagau aacuccugaa gagaaugcag auugguccau cgccgaccuu     3300

gacuaucugc auaaggucau gaacuuucga aaaauuaccg uuacgagacg cuugaaggau     3360

cagaaggggc aacucuacga cgaaucuagg uaucccagcg ugcugcacga ugcgaaaucc     3420

aaggcuucaa ucaauuucga caaacauaag ccaguggauc uauacggagg cuuuagcagu     3480

gccaaacccg cauacgccgc ucugauuaag uucaagaaca aauuucgucu uguaaauguc     3540

cuccggcagu ggaccuauuc ugacaagaac uccgaggauu acauccugga gcagauaaga     3600

gguaaauauc cuaaggcaga aaugguguug agccacaucc cauacgggca gcugguuaaa     3660

aaggacggcg ccuuagugac aauuucguca gcgacugagc uccacaauuu cgaacaacug     3720

uggcuuccgc uggcugauua uaaguugauc aacacccucc ugaaaacaaa ggaggacaau     3780

cuagucgaua uucugcauaa caggcuugac cuccccgaaa ugacuaucga gucugccuuu     3840

uacaaagcau ucgacaguau acuguccuuu gccuucaauc gcuaugcuuu gcaccagaac     3900

gccuuaguga agcugcaggc acaucgagau gacuuuaaug cucucaacua cgaggauaaa     3960

cagcaaaccc uggaaagaau uuuagacgcc cugcacgcga gcccugccuc uagcgauuug     4020

aagaagauca aucuauccuc aggauucggc cggcuguuca guccaagcca uuuuacgcuc     4080

gcagacacag augaguucau cuuucagucu guuaccgggc uguucuccac ucagaaaaca     4140

guggcucagc uuuaccaaga aaccaaguga                                      4170


<210>  3236
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3236
augaagaacu acaauaucag ccuggugccc uccccuucug gcgucgggua ugccguuacc       60

gacgauaacc ucaauauuau aaaaccagug caccauguau caggaaucgg uaguagacuu      120

uucaacgagg gcagcuccaa gcaggacagg cgcaauucuc ggucggcucg acguucaaga      180

cacaggcaac aggggcggau uaaagcacug aacagcauca ugaaggauga aauuaucaaa      240

guggacccga cauuuuucga ucgcuuguac cagagugagu uaucaccccu ggacaagaau      300

aagaaauaua gaaacauaau cuuugauaag ccuucugucg aaagcuacua uuacaaaaag      360

uucccaacua uuuaucaucu agagaaauac cucaucaaua cggacggaaa ggccgauauu      420

aggcugaucu acugggcgcu ucacucccug uugacccauc ggggccacuu uuauaacaca      480

acucccgugu cacaguucaa gccuggugcu cucaauauaa aagacaaguu uauugaacug      540

aaccaacuga aucagguuaa aaccuucgag uuuagugugg ccaacacacc agagaucgaa      600

cacauucuca aggaucgcua caucagcaaa cagaauaaga ucaaccaguu agcagagcug      660

cuuuucgaac aagagcccga gaaagacauu cagaaggcca acaccaaggu cgcuaaaacu      720

auagcaaagg ccaucuuggg gaauaaauuu cagcuggaug aaauuaucgg acuagacgug      780

aacaagucug agaaaaagga auggucccuc cagcugucag accuuaaucu ggauagcaag      840

auagacgagu ugagugauuc ucucacagac caacagcugc gaauuuuaga aauccugcag      900

gagauguaug cugccaccac gcuccuugau auccugcaag gcuccgacag cuugcuagau      960

gcgaaaauua agucguacaa cgaguauaaa cagaauagac uccuguucuu ugaccugcuu     1020

aagacucugc cugauaaaaa ggcacauuug cucaggcagg gcuacacccu guauuuaaac     1080

aaucaccauc gggaccuggc cucugcucgu aaggaucuag ggaucguagc cgcaacaaac     1140

uucuccaaaa cugaccucua cgcucugauu aagaaaaccc uuaaggacga accagagaca     1200

ccggaaucca aaaagguuuu gggacugaua gauaagggug aguuccucaa uaaacagcgc     1260

accgaccuga acacguacau ccccuaucaa cuuaaugccc ugauuuugaa caagauccuc     1320

aaaaaucagg gcaagcacua cccuuuucug aucaaagaaa acccagcgga gcccgauaga     1380

aaggaggccc cuuaugaaau uagucaguua augcaguuca cuauaccaua cuauugcggg     1440

ccccuggcaa caccggagga acaaaagaau cuaccugaga gcuccagguu uggauggcuc     1500

gugcggaagg aagagggccg aaucaccccc uggaacuucu acaacaagau uaaugucauc     1560

gagacugcua acgccuuuau uaaacgcucu aucgguaagg acacauaucu gcuuagcgaa     1620

ccaguguugc cugauaauuc acugcucuac cagaaauaca aggugcugaa cgaguuaagu     1680

uccauaagcc uggucgacgc uaauaccaaa aaggaaaaga aacuuacggc caagaucaaa     1740

caguugcucu auaaggaggg guucaaaaag cacguuaccg ugucuaagac aauggcacug     1800

aaaauucuaa acgauaauaa cguacccacu ucagacaucc ugggacucag uaccggcaag     1860

aaauuugaua auagccuguc cacauacaac ucuuggaaga aagccuuccc agagcagauu     1920

gacaaucccc auuauagaca agaucuugaa uacaugaucg aguggucgac uguguuuacc     1980

gacaggaaga uauuggucga aaagcugaaa gagauugaau gguuacggcc ugagcagauc     2040

aaguucgugc ucgagcaccg ccugacaggc ugggggaaac uuagcaagag acuguugguu     2100

gaacucaaag auaacaaggg aaaauccaau auugacaacc uguuuaauac caagaagaac     2160

uucaugcaga ucgugucaca gcaagucuau aacacucaga uagaggcuau cgcgcuaaaa     2220

acgacaaaga aucagagucu ggacaacauu cucgaagcag ccuacaccuc uccagcuaau     2280

aggaaagcca uccgucaaac uaugaaggug guaaacgaga uuguuacccu ggcagguaaa     2340

ggcacacauc cggauaagau cuuucuuacc uuccagcgga gcaaggaaca ggaggggaaa     2400

uugacuacag accgagccuc cucacuguua aagauauaua gaggaauuaa agauccccuc     2460

uguacgaagg agcuggugga cgaacuuagc aaaaauguca gggcuaaccu gaucaagacc     2520

aaggaguacc uauacuauca gcaacucggc cgcgaugccc ugacugguga aaucauugac     2580

aaaacagauu ugcggaauua cgcagugcug cacaucauuc cuagaucugu cgugguugac     2640

gauuccauaa acaauuuagu gcucaccaag guaaagaacg cgaaggagag uaucgucgug     2700

caguauggga gcaaaaauau uucugacauc uccaugucag uuaaggauuu cuggaacagg     2760

cugcagaaac uuggccuguu uaguaaagga aaguugcgca aucucacaac ugaccugacc     2820

gaacuaaacc aguacgagaa acaaggcuuc auugcucgac agcuggugga gacgaaucag     2880

aucgucaaac uucucgccac cauacugcag gggcaauuuc cacauacaaa aaucauugaa     2940

gugcggcagg agcagaucgc aaacauucgu uaucaauugg auaucuacag acugagggac     3000

uuaaacguau auuaccgcgg aauggacgcc uaccucgcug ccgugguugg uacuuaucug     3060

uacaccgucu aucccaaggc acggagauuc uuuguguacg gcaaguaucu uaaacaggau     3120

aagaauaaca gggaagaggg gcaguucaaa acaagcggac acucguuuaa uuuccugugg     3180

aaguuguuuu acggcgguga ggacgaaaua uaugugcagg ggacuaacaa uauugcuuuc     3240

aacaaaaagg aucuccugca gaaaaucaag gaggucaaua acuacaaguu ucaaaauguu     3300

ucccuagcca ccggcacgcg acgcucugac cuguucaaac agacaaucua cccucggcag     3360

gaaagagaug ugaagaaaag caggacccuu auuacuaaga aaaacggacu cuuuacagac     3420

auauauggcg gguacaccgg aaaugugaac ucauauuucg uacuggucca aacuaaguac     3480

aagaaagauc cagacaaguu caauuuguau ggugugccca accguuacgu ugcgauguua     3540

gagcagagua aauccaaggg caauuaugaa accaaacuga aggaggugau ggagccgauc     3600

auuaagaacu cuaauaaacg caugaucggg uacaagauuc ucaaagaucg gauccuguac     3660

aaccagauaa ucgaagacaa cggauauaag uuuuucauua caagcuccca guacagacac     3720

aauuuuaggc aaaugauccu uucagaucag agcaaaaaga uucugaugga cuaugucuuc     3780

gacccuaacu uuuaccgaca uaagaaaacg gaggauguga auaacgacaa ggaugccaaa     3840

uugaucaagg uuuaugacga aauacuauac cagauugauc acuaucugca gaucuucacc     3900

aucggccauc acauugacaa acucaagaaa gcucggccac uguuugagaa gugcacucuu     3960

gcagaaaagg cccaaguguu aaaugauauc cugauaauug cugguaguaa ccagacagag     4020

cucgccuuga aaccccugaa gaccacaaaa cucacuuucu cuaagcagaa uuguccucug     4080

accaaagacg caaucuuuua cuaccaaucc cccacgggga ugauuacacg caaggagagc     4140

aucaaggaua uacuuaaaca gaacauuuga                                      4170


<210>  3237
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3237
augagcagaa agaacaucgu ggaggacuac gccauuuccc uggauauagg caaugcuucu       60

gucggguggg uugcauucac ccccaacuau aggcucgugc gggccaaagg acacgaacuu      120

aucggugcgc gccuguuuga gccugcuuca acagccgaag aacgacguau ggcaagaacu      180

acgaggcggc gcuacaguag aaggcggugg cgcuugcgaa uguuagacag cauguucgau      240

caggcccugu ccgaaguaga cccaucuuuu cuaaugagaa ggaaguauuc gugggugcau      300

ccggaggaua aaaauaacga ggcucacugg uucggcgggg uccucuuuga cucaaaugaa      360

caagauaagc gguucuacga gaaauauccc accauuuacc aucugcguaa gagccuuaug      420

gaagacgaug agcgccacga caucagagag guguaucugg caauucauca caugaugaag      480

uacaggggaa acuuuuugac agaaggcgag cucgccccua guuccguuuu cgauguggaa      540

gacuuucuga aacugcucac ugagaucuua caguacgcug augaagguga gaccgacgag      600

gaccuggaac uucaggccga ugcgaauggg uugacagagg cacugugcac cacuaaggga      660

ucucggagca uacgagccaa aaacgcuauc uccauucuaa aggaaggcgc cgcacucuca      720

aaacgccagg aggcuaucau uagugccguc uucgcgggcc uggaagggaa uaaacuugac      780

cugacaaagg uguuuccaaa caaagaguau acccccgagg auaagaaaac guuggcccuc      840

uacuucaaua aggcagacua ugaugaaaag agaguagcua ucggagacag cggucuguua      900

gaggauaacg aaugugacuu ucuggauugc auaaggcggc aauacucugc cauugcucuc      960

aagcagcuuc ugggcgacuu cacuuccauc ucagagagca ugugugcccg cuaugaacag     1020

caccagagaa auugggauuu gauuaaaagg uacugccgug aggagaguaa cgcagaaaau     1080

aucaacaaga acuauggggc ccuaguugga ugggagaucg ugggcgguca acggcgaucu     1140

auuagagggg cuaaggacua cgaaaauaaa aggaaggcgg caaacaaauu cuuugaggag     1200

cugauaaagg gauccgcccu cccugaccag gaaaaagagc gccugcuuca ugauaucgaa     1260

aaugacgagc uguucccagu ccagcgggau agcggcaacg gcgugauucc cuaccaguug     1320

caccucaaug aacugaagaa gaucauacaa aaacagggga gauauuaccc uuuucugcug     1380

gagaccuaua uuaaggacgg aaaagaggug aacaagcuag aaggucuccu ggcuuucagg     1440

gucccauacu auguuggccc gcuugugucg cgcgaugaca ugcagucuuc cgauaaucga     1500

gagaaccauu ggaugauccg gcguaaaaag gggguaauca cacccuggaa uuuugacgaa     1560

augguggaua aggacaaauc aggacacgag uucauuaaga guuuggucgg cacugauagc     1620

uaccugcucg gugaaaccac acugccuaaa aacucccuuc uguaucaaga guuugaggug     1680

uugaaugaac ucaacaaugu uagacugucu aucaggaccg ggaaccacug ggccgacaag     1740

cgccggguga gauuaggcca uaaugagaaa aagcugcuac ucgaggagcu guucgcaaag     1800

caggccacgg ucacuaaaaa ggcugccgaa aaccuuuuga ggcgaacaua cggaaacacc     1860

uacgagauua gcggccuggc agaugagaaa aaguuugugu caagucucuc cagcuauggg     1920

aaaauguguc gcacucugaa ggcgucuuac guaaauucac acaaagacgu uguggaaaag     1980

auaaucgagu uacagacagu cuucgaagac aagaaaaccc ugaagcauca gcuucaauug     2040

cucggagagc uggcugagac gaacugcgaa cuacugagua auacccacua uacagguugg     2100

ggcaaacuca gccggaagcu gcuuacuucc aaaguggucg uguguaagau ugggaacgau     2160

uuugccccag caaagcauuc uaucaucgag auuaugagag aaaccgacag gaauuucaug     2220

gagauaauca cagauaaaga cuugggaguu ggcgaaugga uuaaccagca gaaugugggu     2280

gccgaggaug ggaacucgua ccaggacauc auugaugacc ugcguguaag ccccaagguc     2340

aaacgcggca ucaaucaagc uauacgggug aucgaugaca uuuccaaggc cguuggaaaa     2400

gagccuucaa gaaucuuuuu agaacucgca ggcgaugugc agcagagugc uaggacuguc     2460

ucucgaaaga gccggcugca ggggcuuuau aaguccgccg gacugcaaaa agaguuccgc     2520

gacuuggcgg aaucacucaa cgagugcagc gauaaugacc ugagagacga uaggcuauuc     2580

cuguacuaua cccagcucgg uaaggacaug uacacaggcg aaccacugaa ccuugaucgg     2640

uugucuuccg acuacgauau ugaccacauc auaccccagg ccgugaccca gaaugauagu     2700

cuggacaacc gaguauuagu ggcuagaguu gcaaaugccc gcaaaacuga uagcuuucaa     2760

uaucucccgg agauugcuaa caggaugcgu aacuucuggc aggagcugcu ugacgccggg     2820

cugaucucuc gggucaaguu ugauagacua aauaggcaga acgacuucuc cucacacgaa     2880

aaagagcgcu uugugcaacg aagucucgug gaaacgagac agauuaugaa gaaugucgca     2940

acaaucaugc ggaagaggua cggaaacagc gcggccguua uaggcuuaaa uucggaguug     3000

accaaagaaa ugcgccagua ucuggguuuc ucccauaaga accgugacau caaugauuac     3060

caccaugcuc aggacgccuu augucucggg auugcaggcc aauuugcugu gaacagagga     3120

uucuuugcca auggcauggu gucugauggg gcaguaaaca gcuauaaucu guaccuucag     3180

gacuaucugc ggggauacag ggagaaaaug ucagccggcg agcgccgaag aaacaaggcu     3240

uucgguuuua ucgucgggag uaugaacucc caggauucug acaaacggau uaauccuaag     3300

acuggagaaa ucgcguggga ugacgaggau aaaguguacu ugaggaaggu uaugaauuau     3360

cgcaagaugc ucauaaccca gaaagugggc gacagcuucg gugcccugua caacgaaaca     3420

agauauggca ccuccgcaaa ggaggggguc aacggaaucc ccuuugauaa acacaagcaa     3480

gacacugcuc uauacggcgg guucucaagc gccaaaacag uguauguugu gcugaucgag     3540

gccaagaaga aaacgaggcu uguaaauauc accaugcagg aauacgcuga gcucggagau     3600

cacccaagug acgacgcacu gcgggccguc uuggcgaaga aaaagccuga auauggcaaa     3660

gcucguauuc uguuacgcca uauuccccca augcagcuca uucgauacgg uggggcccug     3720

gugacuguga agucugcaac agagcuuaac aaugcccagc aacugugguu gcccuacaag     3780

gaauauugcc ucuucgauga cgucauaucc agcgcuaucg auguugacga ggcaagacug     3840

ugucagaugc uagaugugau ucuggacgcc auccagaaau acuauccuaa gcaaagguuu     3900

gaugagaccg gacuuucggc guuccacaac gcuuuuaaug aaguaucagc cgaggacaaa     3960

aagcggauug ugggcgaaau ggucgguaug cuccaugcag augccaaaaa cgcuaaucug     4020

aaggccuugg gcaugucugg gcgcugggga agaaugagua accaguccgg cuacacauuc     4080

gcagacagcg augaguuuau cuuccagucu ccaagcgggu uauuugaaaa gaggauaucc     4140

gugggagagc ugaaaaagcg gcgagcuaga uga                                  4173


<210>  3238
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3238
augaaggagg ugaaagacua caucauuggc cuggauauag ggaccaacag cugcggauau       60

gucguuacag acaagcagaa uaacauccuc aaacuuaagg guaaaacugc cauuggcgcu      120

agacuguuca aggaagggga ggcagccgcg gauaggcggg cuuuucgcac gacccgacgu      180

agauuggcca ggcggcgcug gagauuagga cugcuagaag agaucuucga cgaagagaug      240

gcaaaggugg auuccucuuu cuucaggcgg cucaaagagu cagacuacag ucccaaggau      300

agccgcaaac aauuuaauuc cauuguauuc gaaucuucgg aggccgacaa ggauuuuuau      360

gacaaauacc cuacaaucua ucaccuucga aacgcucuua ugcaugauga ccagaagcac      420

gaucugagag aaauauucuu ggcagugcau cacaucguca aguacagggg caauuuucuc      480

cgggaggacu cagugaaagc cuucaaggcu gccaaauuua cucugcaggg ugaggacggg      540

auuggaccag uugauaagcu gaacgaccuc uuaaaagaaa ucuauauuga ggcggcaccg      600

gaacuggcca ccgauaaucu uagcaagauc gaggaaauaa uuaaagacaa gaaguuguac      660

aaacaggaua agcugaaaca aguggcuaca cuacucacca aggaggccga cacuaaagau      720

aaggcaaagc ugaacaaaga cgucgcuaaa cagguggcca augcguucau gggcuacaug      780

aucauuuuug auacaaucuu caaccuuagu gacguagauu ccaaagacua uaagguuaaa      840

uucucugacg ccaauaucga ugagaagcug gacaccuuga cgagccuccu gacugauaag      900

cagaccgaau uuguguuaga gcugcagaac auuuacaaua caauaguccu caacgaaauc      960

gugcccgacg gcaaaucccu uucagagagu auggugaaga aauaugauga ccauaaggaa     1020

gaucugaaau uguucaaggc auaccuaaau agccugacug acgucaagaa aguuaaggag     1080

cucaaagcug ccuaugugcu guacguaaac aaccguaagg cuaaucuucu ggccgcaaag     1140

aaguugcucg ggaaaaaggc cgauaacaug aauaacuuug agauuuucuc uaaguuugug     1200

gcugacaauc uggaugacuc cgaauuagcg aacaaaaucc gcgcaagacu ggaucuagga     1260

gaguuccucc cuaagcaaag gaccaaucag aacgguguca uaccauauca gcugcaccag     1320

guggaacuua cacaaauuuu ggagaaacag ggcaaguacu accccuuucu gaucgccccu     1380

aauccaguug aaucacaccg gaacaaugcu cccuaucgaa ucagcgagcu cgugaguuuc     1440

cgcgucccuu acuauguggg gccacugauu gacccgaaca augccgaguc ugaacaggac     1500

aaagcaaacu ccagcuuugc cuggauggua agacaaaagc agggacagau cacccccugg     1560

aauuucgagg aaaugguuga uacgacugag ucggcuaaca aguuuauuaa aaggaugaca     1620

accaaggaca cuuaccuucu gggcgaggau guguugccug ccucuucccu cauauaucag     1680

aaauucguca uccugaacga auuaaauaac auuaagguga augguaucaa acugacauca     1740

gaggaaaagc aucaaaucuu ugacgagcua uucaaggucc agaaaagugu gagccucaag     1800

aaccugguua auuuccuuac cgcacacggg uguguggcgc caaacguaac gggauugucc     1860

gauggcacca aauuuaauuc uagcuacuca acauauauug acuaccggaa gauauucggc     1920

gcugaaaucg auaacaaaga caagcaggcc gauuuugaga agaugauuga guggaguacu     1980

gucuucgaag accgcaaaau cuuuaagaga aaacuggagg aaauuaccug gcucacaccc     2040

gaucagauca agcaaguguc cagcaaaagg uacucugggu ggggacgucu gucaaagaaa     2100

uuacugacuc agauaaccga cgagaauggu guuaacaucc uucagcgguu guggaaugaa     2160

agugagaacc ucaugcagau ucugagcgau ccugugauca aggagaagau ugucgcagcc     2220

aauuccucuu ucacaaaagc uaccgacaug gaagacaucc uagccgaggc auauacuucg     2280

ccacaaaaca agaaagcuau acgacaggug augagaguag uggaugacau uaucgccgcg     2340

gcccauggca agaaacccag ccagauugcu aucgaauuug caagggagga ucaggacaau     2400

ccgcaaauga cgaagucccg caaggcccag cuggaucaga uauacgcuaa aaucucagac     2460

gaauuucucg auaacgccgu uaagaacgag cugaaaaaua ugaaggacaa caaaccucuu     2520

gcaaaggaua aggucuuuuu guauuucaaa caaauggggc gggacgcgua cacaggagag     2580

aagcugaguu uagauaaucu ccaggacuau gacauugauc acaucuaccc ccagucuuuu     2640

auuaaagacg auagcuucga caacagagug cuggugcaga gggccgucaa uaacggcaag     2700

uccgauaaug uuccagugcu ucuguuuggu aacaaacaag cugccgacuu ggggaugacc     2760

auccgccaga uguggaagaa guggcucgaa cugggccuaa uaucaaaacg aaaguucaau     2820

aaccugauua cugauccuga gaaaaucagc ucuuauaugg cauccggauu cgugaaucgg     2880

cagcucguag aaaccaguca ggucaucaag cugguggcua acauucuuca agccaaauug     2940

cccgacacag agaucauuga gguuaaggca agcuacaauu cuauacugcg uaaggaauuu     3000

aacuuauaca aauccagaga ggugaaugau uaucaccaug ccaucgacgc uuaccucacc     3060

acuauugucg gcaacuaucu guaccaggug uauccaaagc ugaggcccua cuucguuuau     3120

gggcaguuua aaaaguucgg aaaagcggaa ucaaaggagg augacccugu gaaaaagauc     3180

aagcaguuua acuucauuua ccaacugcua ugggguaaag augacgccau cugcauaagu     3240

cacacagacc agcagguauu uagccgcucg gauaucauua auaagcucac gaccgcauac     3300

aacuucaaau auaugaaugu cucccaagaa guguacacua acucuaauaa cguguuuaag     3360

cagacagucu ucccaaucau ugagcgggac accgcuaaaa gcagaucacu gaucccgaag     3420

aaguuggaua ggcccacaga cauauauggc ggguacagua auaacaauga ugccuaucug     3480

gccuuaguua aaguggacaa gaaaaaggga uccgaguacc gaguaguggg cauuccuaug     3540

cgcgcugcag ccaugcucaa agcgucuaag aacuaugaug aagaccugcg gaaggucauc     3600

gagccaaugg ugaugcuuga uaaaaauggu aaggcuaaaa gagggaucga aggcuuuagg     3660

guucuggugg gaaagaugcc cuucaaacag gccgucuugg acggcgauaa gaaguucaug     3720

cucacuggga ccaacgacau gccuaaucug aaacagcuag ugcugagcca acagaacaug     3780

aaguacauuc uugauuacau agacgacauc gauugucgua aacauaaguu uggaaauaaa     3840

acgcaggacg guaacaagug ccuccuggag guuuucgaug aaauuaucca gauuguggac     3900

aaacacuuug aguuguucga uaaucgcucc uuucgggaga agcugagaaa cucaaggguc     3960

aacuucauca auuuagcacu cagcaagaaa gccgaagcuc ugcuugcaau acugaacggc     4020

uugcaugcca auaguaugcg aaccacaauc aaggagcuca aaauuucuga ccuggggcgg     4080

auccaaucca gcguaauuuc gccagaagcg cagcuaaucu aucagucacc cacuggacug     4140

uuugagcgca gagugaaggu uaaagaucuu uga                                  4173


<210>  3239
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3239
auggagaaca agcaguacua uaucggccug gacgugggga ccaauagcgu cggaugggcc       60

guuacagaua cuuccuacaa ccuccuuaga gcuaaaggua aggacaugug gggcgcaagg      120

cuguucgaaa aagccaauac ggcggcugag cggcgcacca agcgaacauc ucguagaagg      180

ucagaacggg agaaagcccg caaggcaaug uugaaggaau uauuugccga ugagauuaac      240

agaguggacc ccaguuucuu uauaaggcug gaggaaagca aauucuuucu agaugaccgg      300

uccgagaaua accgccaacg auauacucuc uucaaugaug cuaccuuuac agacaaggau      360

uacuaugaaa aauacaagac caucuuccac cugagaucug cacuuauuaa cucggacgag      420

aaauuugaug uaaggcuggu guucuuggcc auccucaauc uguuuucaca ucgggggcac      480

uuccugaacg cuagccucaa gggagacggc gauauucagg guauggacgu cuuuuauaau      540

gacuuagugg agaguugcga auacuucgag aucgaacugc cucguauaac uaacaucgau      600

aauuucgaga agauucuuuc ccagaaaggg aagucucgca caaaaaucuu ggaggaacug      660

agcgaggaac uauccauuuc aaagaaagac aagaguaaau acaaccucau caagcugaua      720

agcggacuug aggccucugu uguggaacug uauaauauug aggauaucca ggacgaaaac      780

aagaaaauua agaucggcuu uagagagucc gauuacgagg aaucgagcuu gaaagucaag      840

gagaucauug gcgacgaaua uuucgaucuc guggagaggg cgaaaagugu acaugacaug      900

gggcuguuau cuaacauaau cggaaauucc aaguaccugu gugaagcacg gguugaggcc      960

uaugagaacc accauaagga ucuccuuaaa auuaaggaac uguugaaaaa guacgacaaa     1020

aaggcuuaua augauauguu ucgaaagaug accgacaaaa acuacagcgc cuacgugggu     1080

ucggucaauu cuaacaucgc aaaggagcgc agauccgugg acaaaaggaa gauagaagau     1140

cuauauaaau acauugagga cacggcucug aagaauaucc cagaugacaa caaggauaaa     1200

aucgagauuc ucgaaaagau caaacugggc gaguuccuua agaaacaacu gacugccuca     1260

aaugggguga uuccgaacca guugcagagu cgggaacucc gcgcgauacu gaagaaagcc     1320

gagaauuauu uacccuuucu gaaggaaaag ggagagaaaa accuaaccgu cagcgagaug     1380

aucauucagc uuuucgaauu ucaaaucccu uacuauguug gcccacugga caagaauccc     1440

aaaaaggaua acaaagcaaa uuccugggcu aagaucaagc agggugggag gauucuuccu     1500

uggaacuucg aggacaaagu ggauguaaag ggaucuagaa aagaauuuau agagaagaug     1560

gugcguaaau gcacauacau cagcgacgaa cacacuuugc caaagcaguc acugcucuau     1620

gagaaguuca ugguccugaa cgagauuaau aacaucaaaa uugauggcga aaagaucagu     1680

guggaggcca aacagaagau auacaaugac cuuuuuguua aaggcaagaa ggugucccaa     1740

aaagauauca agaaagaacu gauuagcuug aacaucaugg acaaggacuc uguccucuca     1800

gggaccgaua caguguguaa ugcuuaccug aguagcauug gaaaauucac cgguguauuu     1860

aaggaggaaa ucaacaaaca guccauaguu gacaugauug aggauaucau uuucuuaaag     1920

acgguguaug gcgacgagaa gcgguucguc aaagaagaga ucguggaaaa guacggggau     1980

gagauagaca aagauaagau caaacgaauc cugggauuua aguucucuaa uuggggcaac     2040

cuaucaaaga gcuuucucga gcuggaaggu gccgacgucg ggacuggcga ggugagaucc     2100

auuauucagu cacuuuggga aacaaauuuc aacuugaugg agcugcucag uucuagguuu     2160

accuauaugg augaacugga gaaacgcguu aagaaauuag agaagccccu gagcgaaugg     2220

acuaucgagg accuugauga cauguacuug uccucaccug ugaaacggau gauauggcaa     2280

agcaugaaga uuguagauga aauccagaca gucaucggau augcaccaaa gagaauuuuc     2340

guggagauga ccaggucuga aggcgagaaa guucgcacga agucccgaaa agaccggcuc     2400

aaggagcugu acaaugggau caaagaagac aguaagcagu gggugaagga gcuagauagc     2460

aaagacgaau cuuauuuucg uuccaagaaa auguaccugu acuaucucca gaagggaaga     2520

ugcauguacu caggugaggu cauugaacug gauaaacuua uggacgauaa cuuguaugac     2580

auagaucaca ucuacccgag gaguuucgug aaggacgaua gccuggacaa uuuaguacuc     2640

gugaagaaag agauuaacaa ucgcaagcaa aacgauccca ucaccccuca gauucaggcc     2700

ucgugucagg gcuuuuggaa aauccugcau gaccaagggu ucauguccaa cgagaaguau     2760

ucucggcuua caagaaaaac ucaggaauuu agcgacgagg aaaagcuguc auucauaaau     2820

aggcagaucg uugagaccgg acaggcuaca aaaugcaugg cgcaaauuuu gcagaagagu     2880

augggcgagg augucgacgu gguguuuucc aaggcacgac ucgucucuga guuccgccac     2940

aaauucgaac uguuuaagag ccggcuaauc aacgauuucc aucacgccaa ugacgcuuac     3000

cugaacauug uuguggguaa uuccuauuuu gugaaauuca cuagaaaccc agccaauuuu     3060

aucaaggaug caaggaaaaa ccccgacaau ccuguauaca aguaccacau ggaucguuuc     3120

uuugaacgcg acgucaaguc aaaaagcgag guggccugga uagggcagag ugaaggcaac     3180

ucuggaacca uuguuaucgu gaagaaaaca auggcuaaaa auuccccacu caucaccaag     3240

aaggucgagg agggccaugg gagcauuacu aaggaaacga uagugggagu uaaagagauc     3300

aaguucgguc ggaacaaagu ggaaaaggcc gauaaaacac ccaagaagcc gaaucugcag     3360

gcauauagac cuauuaaaac cucggacgag aggcuuugua acaucuugcg auacggcggg     3420

cggacuucaa uuucuaucag uggauauugc cugguagaau acgucaagaa acgcaagacc     3480

auaagauccu uagaggcgau ccccguguau cucggcagga aagauagccu gucugaggaa     3540

aagcuccuga auuacuuucg guauaaccua aacgacggug ggaaagauag cguguccgac     3600

auucgacucu gucugccauu caucucaaca aauaguuugg ucaagauuga cggcuaccug     3660

uacuauuuag gaggcaagaa cgaugacaga auccaacucu acaaugcuua ucagcugaaa     3720

augaagaaag aggaaguuga guacauacgc aagauugaaa aagccgugag caugucuaag     3780

uuugaugaga ucgacaggga gaagaacccu guacuuaccg aagagaaaaa uaucgaacug     3840

uauaacaaga uucaggauaa auucgagaau acuguguucu ccaagcguau gucauugguc     3900

aaauacaaca agaaggaccu caguuuuggg gauuuccuga aaaauaagaa aagcaaguuu     3960

gaggaaaucg accuagagaa acaaugcaag gugcuguaua acauaauuuu caaucuuucc     4020

aaccucaagg aaguugaucu gucugacauc ggaggcucga aaagcacagg uaagugucgg     4080

ugcaaaaaga auauuacgaa cuacaaagag uuuaaguuga uccagcaguc aauaaccggg     4140

cuguacuccu gcgaaaaaga uuuaaugacu auuuga                               4176


<210>  3240
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3240
augaaggaga ucaaagacua cauuauaggc cuggauaucg ggaccaacag cugcggauau       60

guggucacag acaagcagaa uaacauucuc aaacuuaagg guaaaacugc caucggcgcu      120

agacuguuca aggaagggga ggcagccgcg gauaggcggg cuuuucgcac gacccgacgu      180

agauuggcca ggcggcgcug gagauuaucc cugcuagaag agauuuucga cgaagagaug      240

aauaagguug aucccagcuu uuucaggcgg cucaaagagu cagacuacag uccuaaggau      300

cgccgaaaac aauuuaacag caucguguuc gaauccucug aggcagacaa ggccuuuuau      360

gaaaaauacc caacaauaua ucaccugaga aaugcucuua ugcaugauaa ccagaagcac      420

gaccugaggg agaucuucuu ggcaguacau cacauuguga aguaccgggg aaauuuucuc      480

cgugaggauu cggucaaagc cuucaaggcu gccaaauuua cucugcaggg cgaagacggu      540

aucgggccgg uggauaagcu gaacgaccuc uuaaaagaga uuuauaucga agcggcaccc      600

gagcuggcca ccgacaaucu uucaaagaua gaagauauua ucaaagacaa gaaguuguac      660

aaacaggaua agcugaaaca aauugcuaac cuacucccua aggccguuga cagcaaagau      720

aaggcaaagc ugaauaaaga caucgcuaaa cagguggcca acgcgcuuau gggauacaug      780

uuccgccugg auacauuguu uaguuucacc gacgucgaug ugaaagacua uaagcucaaa      840

uucuccgaug ccaauaucga cgagucucug gacgcauuaa cuagccugcu cacagaugcu      900

cagauugagu uuguacuuga acugcagucc auauacaaca ccaucguuuu gaaugagauu      960

gugccagacg gcaugucacu aagugaaagc auggucaaga aguaugauga ccauaaaaag     1020

gaucugaaac ucuacaagga guauaucgac ucucuguccg aucaaaagaa ggccaagcag     1080

cuugaagcug ccuacgcacu guaugugaac uacagaaaag ccgacuugcu cgcugcgaag     1140

aaacuguucg agaagaaaaa gauaggcgau aaaaaguuug uggacgucau uucaaacuuc     1200

gagguuuuug ggaaguucgu gagcgauaau uuagacgaua gugaacuggc aaacaaaauc     1260

aaggccaggc uagaccucgg agaguuucug cccaaacagc ggacgaauca gaacggugua     1320

aucccuuacc aacuucacca gguggaauug acucagauuc uggagaagca aggcaaauau     1380

uacccauucc ucaucacccc caauccuguc gaaucucacc gaaacaaugc uccauaugag     1440

auuuccgagc uggugagcuu ucgcguuccg uacuaugugg ggccccuuau agacaaccag     1500

ucgaucaagg auaagcagaa uaaaaacaag uucgccugga uggucagaca gaaacaagga     1560

cagauuacac cuuggaauuu ugaagagaug guggacacua ccgaaucugc aaaccaguuc     1620

aucaagagga ugacacggaa agauaccuac cuguuggccg aggacguacu cccaaagucc     1680

ucacugaucu aucagaaguu uaugauuuua gaugagcuga aucgcauaaa aaucgacggc     1740

aagaaacuaa cgaguaagcu caaacaugau auuuucgaaa agcuguucaa gaaacaaaag     1800

agcaucaacc uugacaauuu gaaaaacuac cugcucgcug aggguaacau ucccgggcug     1860

aucgaaggau uauccgaugg cauaaauuuu aacaauucuu ucagcacuua caucgacuau     1920

agaaacauuu uuggcgauga gaucgacaau ccuaacaagc aggccgauuu cgagaaaaug     1980

auugaauggu caacaguuuu ugaggacagg aagaucuuca aacguaagcu gaaggaaaua     2040

accuggcuua cuccagagca gauuaaacag gugaguucca agcgguacag cgggugggga     2100

cgauugucua aaaagcuccu gacacaaauc accgacgaaa auggugucaa cauucuacag     2160

agacugugga augagcccga aacgcucacc gaggugcugg caaacccggu caucaaaagg     2220

aagauaucag aggcgaauag ucuuuuugug cagaucaaca agguugaaaa uauuuuggau     2280

gacgcuuaua caagcccuca gaacaaaaag gccauccgcc aagugauucg gguagucgau     2340

gacaucauag cagccgcuca cggcaaaaag cccucccaga uugccaucga guucgcaaga     2400

ucuucgcaga auaaagcuaa ggugccagau acuaggaaga aacaacugga caagaucuac     2460

aacaaaauua gcuccgaaau cuuagauuca aguauuaaga acgagcucaa aaaucugaag     2520

ucuaacaagu aucuuagcaa agacaagcug uuuuuguacu ucaaacagau ggggcgcgau     2580

gccuauaccg gagacaagcu cucccuggau cagcuacaga auuacgacau agaccauauc     2640

uuuccucgau cauucauuaa agaugacagc cuggauaacc ggguucucac acaaaagccc     2700

aucaaugcga agaaaucuga cuacggcauu ccagcccugg aauuugguaa caaguaugug     2760

cccgaucuug ggaucacugu caaagagaug uggaaguugu ggcaggagaa uggccugaua     2820

uccaaaagua aguuaaucaa ccucuguacc aauccuaaaa agauuggaag caagcgugcu     2880

ucuggcuuca ucaacagaca gcugguggaa acaucccagg uaauuaaacu uguggcaauc     2940

auacugcaag ccgaguuccc agacaccgaa auuaucgagg uuaaggcucu acagaauacu     3000

acgcucaggg agucauuuca ccuguacaaa aaccgcagug ucaaugauua ucaccaugcc     3060

aucgacgcau acuugacaac cauugugggg aacuaucugu accaggugua uccgaaguua     3120

cggcccuacu ucgucuacgg acaguuuaaa aaguucaauc aagaaaagaa cauagauauc     3180

cucaaaagac ugaagaacuu uaauuuccuu aggcagcuga uuuuuaacac ugacgauaau     3240

aucuauauua gcgguaccaa agagaucguu uucaacaaga aagacauaau ccacaaguug     3300

gaaacagcgu acggcuauaa guacaugaau auuucgcgag agugcuauca ggaaaccucc     3360

ucucucuuua accaaacucu guacgcccau aauagcucag ugaaaaacag ucuaaucccu     3420

aagaaaaagg ggcugccaac agagauuuau ggaggcuacu ccgguaauaa agauucuuuc     3480

uuugugcuug uaaagaucgu caagaaacgc acgaaccucu accggauagu ggggauuccc     3540

accagagagc uggcuaaguu gaauagcucc aacaauuaua accaggcccu gaauaaaauc     3600

guugaaucaa aguuaugucu caaagagacu gaaucuuuca agauccugau uaaaaggcuu     3660

cuguacggca cauugaucgu ggacaacgga cagaaguucc guauaggcag uuuuaaggag     3720

aaacacaaug uccagcaacu cgugcugcag cuaaagucua ugaaauauau uaaguucuac     3780

aucgacgggg gacagaacua uuuuaccgau guugaacgca aaaagcugga gaagcaggac     3840

cgggauaaau gccuucucua cguguucgac gauauuauga acguagucaa uaagagauuu     3900

acacuguucg acauguccaa auaugagaag gauggugaca gcuugaggga aaaauuuaac     3960

uguuuagauu ucaaugacaa ggugucgauc cugucagauc uccugaaggc auuucaugcu     4020

aacucugacc gaaccaguau aacuaaacuu aagauuacga aucugggccg gcaccaagcc     4080

gggaaaaacg gaaucaccuu gacaacuaau gcacagauca uuuaccaguc cccuaccggc     4140

cuauucgagc gcagaaucaa gauuaaagau cuguga                               4176


<210>  3241
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3241
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaac acgagcauga acaguccucu auagucaagu uggguguuag agugaacccc      120

uuaucaguag augagaaaac aaauuucgaa aagggcaggc cucugaguau caacgcagac      180

cggacuagca aacgcuccgc ccgacguaau cuacaaagau uuaagcucag gcggaagaac      240

cugauuaauc uucuguugaa acaccauauc auuucuaacg agacgauccu caccgaggug      300

gggaagaaua caacucacca gacccugaaa cugcgcucga aggcgacaca ggaaaagaua      360

ucacucgagg aauuugcuag aaucuuacug agcauuaaca agaagagggg auacaaaagu      420

ucccggaagg agaaaaauga ggaugaaggc uucaccaucg acgguauggc cauugcaaag      480

gagcuuuaug auaaccaguu gacuccaggg caauacgucu auaauauccu gcaggacgga      540

aagaacuaca uaccggauuu cuaucgcucu gaccuacagg ccgaauuuga ugacgugugg      600

caguuccaaa agaaauuuua cagcuccauu cucacagaug aguucuaucu gaaacuuaag      660

ggccagggca aagaaaauac ccgaaagcug uuuuuggcua uccaucaggu uuacacggca      720

gagaacaaag ggaagagaga cgccgugaaa cuccaguacu auaaguggag gucagaggcu      780

gucaagaaac aacugaguau ugaagaggug gccuacguau uaguugaaau caacaauaac      840

aucaauaaga gcucuggaua ucugggugcg auuuccgauc ggucaaaagc acucuacuuc      900

aacaaugaga cugugggcga auaucuuuac gggcagcugg ucaagaaccg ucacaauagc      960

uugcgcaacc agguguuuua uagacaagac uaccuagacg aguucgagaa aauaugggaa     1020

acccaggcca aguuuuacga ugagcugaca aaugaacuca agcaggagau cagggacgug     1080

gucauuuucu aucagcggaa acugaagagu caaaaacacc uuaucucuga uugccuguuc     1140

gaaaagcauc acaaagcuau acccaagucc agcccuuugu uucaggaguu caagauuugg     1200

cagaaccuca auuaccugga guuuguucau acucagucga aagaaaagau cuucuuuucu     1260

gaguuagacg augaaacccg acaauucgug uuugccgagc ugaacauccg cggaacacua     1320

aaaauuaagg agaucaaaaa gcucauuggc cuggaaaccc caguauauug gaaauccaau     1380

uucgacgaga uagaagguaa cagaacgaau caggugcuuu acaacgucua ucagaacauc     1440

gcagaugacu ugggguacgg auuugauugg ucaaagaagc acgcuuucga gauuaaugcc     1500

gaacugcagg cgaucuuuaa agacgugggc aucgauacug ccauucucaa cuucgacgca     1560

aguauagagg agaaugacuu ugauaagcag aaaagcuauc aacuguggca ccuucuguac     1620

uccgcugaag acacagauaa ggccaccgag gaagacaaau ugaucuacgg caacauuaau     1680

acuaagcuca agaaacagcu gcaucagaag uauggguucg aggcuaaaua cggaaacuua     1740

cuggccaaua ucucucuaca acaggauuau gguaaccuca gcgcaaaggc cauuaaaaag     1800

auccugcccu accuugaauc aggcaauagu uucuaugagg cuuguacaau aguuggguac     1860

aacccuucca auagcuugac caaggagcag caacagaaca ggaaacugaa ggacaaacuc     1920

gaacuguuac caaagaacuc ucugcggaau cccguggucg agaaaauccu uaaucagaag     1980

gugaacuuga uuaaccagcu caucgaugaa uauggaaagc cugacgaggu acgcauugaa     2040

cuggcgagag agcugaaaaa gucagcaaaa caaagggccg aucugaguaa gcacaucaau     2100

gacgcuacgc ucaaaaacca gaauauacgu aagacccugc agaaagauuu uggcauucag     2160

aacccaagca agaaugacgu uauccgguac aaacuuuacg augaguugaa agacaacggu     2220

uauaagacac uguucacuaa uaaauacauu ccgaaggaac aauuauuuuc caaagacgug     2280

gauaucgagc auauaauccc caaggccauu cucuucgacg auucuuuuuc gaacaagacc     2340

cuggcauauc gaaaugucaa ccuugccaaa gcugacgcca cagcauucga uuuuaucagc     2400

uccaagcacc uggacgauuu ggacaauuac aaagcgagag uggaaacucu cuuccaguca     2460

aagaggauua guaaagggaa guauaagaaa cugcuaauga cccgccagaa ccugccugag     2520

gauuuuaucg agcgggaccu cagaaauucu caguacauag cuaagaaagc cacagcaaug     2580

cugaaggaag ucuucaggag cgugguucca accuccggcc gcauuacuga uaaacuucga     2640

gaggacuggg auuuggugaa cguaaugaag gaacugaauu uacccaagua ucggacgcuc     2700

ggacugacag aguuuaucga acguaaaaac ggcaacaaga ucgagcaaau uaucgacugg     2760

accaaaagaa augaccauag gcaccacgcc auggaugcuc uuacugucgc cuucaccaca     2820

cauaaccaca uucaguaccu gaauaaccua aaugugaccc augggcucaa cggaguugac     2880

acugaucacg agaagucacg ccuguacgca auaaaacagg cuaucacaaa gguguauauu     2940

gucgguaaga cgaaaaagcg gaaauuuauc ccuccaaugg aaaauuucag aagccacgcc     3000

aagaaccauu uggagggcau ucugaucucu uucaaaaaua agaacaaagu gguaaccaag     3060

aauaagaaca ggacuaaaac aaagaccaaa gggguguuua augaaaagau acaauuaaca     3120

ccccgaggac agcuccacaa agagacuauc uacggccaug cgaagaaggu uauugucacc     3180

aaagaaaagg uguccaguaa auucacgcac gagaagaucc uacaggugac caaaccggcc     3240

uaucgcgagg cacuucugaa gcgguuggcu agcuuuaaga acgacccuaa aaaggccuuc     3300

ucugguaaaa augcucucuc caagaacccc cuguacuuug auauuacauc agggaaaaag     3360

gucccagaca cuguuaagcu aaguuauuuc gaaaauagcu acgugaucag aaaagaggug     3420

aacucggaua acuuuaagaa ugaaaaaucc cugcagaagg uacuugacgu cggcauaagg     3480

cguauucucg agaaacgccu ggccgauuau aacggaaaug caaaggaagc guucucuaac     3540

uuggagaaaa auccuaucug gaaggacaag gagaaaggcg ugugcaucaa gaaaguucgg     3600

auugaagccc ugaagaacgc ugagcccuua caugugaaaa aggaucaccu cgggaagaua     3660

auccuggacg acgaaaauaa aagcacccca gucgauuuua uuucaacagg aaacaaucac     3720

cauguggcca ucuacgagga caaggauggu aaacuucacg acaagguugu gaguuucuac     3780

gagguagucu ccagagugaa ccugggcuug cccauuaucg auaaaucuuu uaauagccau     3840

cucgacugga aguuccuguu cuccaugaag caaaacgaaa uguuuguguu cccuucagag     3900

gauuuuaauc caaaagaaau agacaucggg aacccgaaga auaacgagcu aauuagcaaa     3960

uaucuguuca ggguccagaa guuuaguaaa guuacuuacg gaaacucugc agugcgagau     4020

uauguauucc ggcaccaucu ugaaaccgag cucaucgaca agaaggagcu gaaaggcauu     4080

acguacuauc aguugaaguc caucgguaug uuagaaaaac uggugaaggu ccgcauaaau     4140

cacauuggga aaaucgugca caucggcgag uacuga                               4176


<210>  3242
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3242
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc ccuggugaaa       60

gaggcugaaa augagaacga agucuccgau auaaucaagu uggguguuag agugaauccc      120

uuaacuguag acgagcugac gaacuucgaa ggcgggaaag cauacucuaa uaacgccgag      180

aggacccuaa agcggggagc gcgccgaaau cuccagcguu auaaacugag aagggagaac      240

cuuauugaaa uccuguugaa gaauggccuc auuaacaagg auacagcucu gacugaggug      300

gguaaagaaa ccacacacca aacccugaag cuccggucaa aaagugccaa ugagaagguc      360

agcuuagagg accuggcaaa aauccuuuug uccauaaaca agaagcgcgg guacaaaucu      420

ucgagaaagg ugaaugccga agaugacgga guugcuaucg auggcaugga gguggcaaaa      480

cugcuauaug acgaaaacau gacuccuggc gaguacgucc ucaggcugcu ugaaucaggg      540

aagaaacauc ugccagauuu uuaucggagc gacuugcagg aggaguucaa ucucgugugg      600

aagaaacaga gugaauuuua cccgcugauu uuaacaaagg agcuguucuu ugaacuccac      660

ggaaagaaca aaucccagac cugggccauc ugcaaggagc cccuugguau uguaggcguu      720

aaacugaagg ggucugcuaa agaacaaaag uucgcccgcu aucgauugag aaaggaggcg      780

cuaacgcagc agaucgagcu ggaacaucuc gcaauagugc ugcaggauau uaacaaugac      840

agcaacaaau ccucaggaua ccuuggcgcc aucagugaua ggagcaagga gcuguuuuuc      900

aauaaagaaa cugucgguca auaccaguau aagcaguugc agaaagaccc uaacaccucu      960

cucaagaauc aaguguuuua ccggcaggac uaucuggaug aguucgaagu gauuuggcgu     1020

aaccaggcua aguuuuaccc acaauuaaca aaugagcuga aacaggagau ccgcgacguc     1080

guuaucuucu aucagagaaa gcuaaaaucc cagaagaacc ucauuucauu uugucaauuc     1140

gaaagcacuc agcagaauua cguggagaaa gaaaccggga agacaaagau aaggaccauc     1200

ggacggcgag uaauuccccg caguucuccu cuguuccagg aguuuaaaau cuggcaaucc     1260

cuuaacaauu ugaaguucgu gaacagcgag ucgaaucaga uaauucaguu uaucgaacug     1320

gaggaugacg ucaaaaaggu gguuuucgau gaacucaacc ugagaggcga ccuuaaacca     1380

aaucaggugc ugaagaucuu gucuaaauau augaagauug gcaagcucac gcaauggaaa     1440

ugcaaccugg aggaaaucga ggggaacagg acuaauuccg ccuuauacaa cgucuuucag     1500

augauugcag aggaagaggg auacgguuuc aauuggggca agaaaucagc ucuggaaaua     1560

aagagugagc uaaaagccau cuuuagcucc auugggauca acgauucuau ccucgacuuc     1620

gauuuuagca agaagcugga ccagcagcuu ucauaucaau uguggcaucu gcucuacagu     1680

gcggaagagg gaaauuccaa aauuacagag gaagauaagu ucaccuaugg caacacuggu     1740

guggcccuga aaaagaaauu acaccugaag uuuggguuca agcccgagua cagcacacuu     1800

auaucuaauu ugcgguuuac cucagacuau ggcaaccuca guagcaaagc aaucuccaag     1860

auucugccuu uccuacgcga cggaucugau uacucgaagg cuugucagua ugccggcuac     1920

aaucacagca acucccuguc agcugacgaa cucgauaaua gagagcugaa gccaaaacuu     1980

gaauugcuga agaagaacag uuuaaggaau ccgguaguug agaaaauccu caaccagaug     2040

gugaaugucg ugaaccaagu cauugacaac uacgggaagc ccgaugaggu gcguaucgaa     2100

cuggcccggg agcuuaaaaa gucugcaaaa gaacgaagcc agaugacgaa gaauauaucc     2160

gacgccacca gaaggaacgc ugagaucaaa aaguaucugu caaaggaguu cgguauuccu     2220

aauccaacaa aaggagaugu uauccgcuac cgguuguggg aagaacucgc gagcaacggc     2280

uauaaggacc uguuuagaga uaaauacauu cccaaggaaa aacuauucuc uaaugagauc     2340

gacauagaac acauuauccc uaaggcacug cucuuugaug acuccuucag uaacaagacu     2400

cuggccuuua aaguggauaa ucuuaagaaa gcugacagga ccgccuucga cuuuaugagc     2460

acagauuucu cuaaggacgu agauuccuau auugggcgca ucgagacuuu gcauaaagac     2520

ggaaagauau caaguagcaa guacaaaaag cugaugaucc caaaaucgaa guuagucgau     2580

ggcuuuauug agcgagaccu ccggaacacc caguauaucg caaaacaggc ccguaagaug     2640

cugcuugaag uguucaagac aguugugccc accacuggua aaauuacgga uaagcugaga     2700

gaggacuggg aucuaaucaa ugucaugaaa gaacucaacu uuccgaagua caaagcucug     2760

ggguugacaa agaagauaga gagguucgac gugggcuccg aaaagauuaa gucuaucaau     2820

guaaucgagg gauggagcaa acgcaacgau caccggcauc acgccaugga cgcacuggcg     2880

guggcuuuua ccacucacaa ucauauucaa uacuuaaacu aucucaaugc cagaaaggau     2940

auccaccaug agaugcacgg caccauuaaa ucaauagaaa accagaucau ugggaaggac     3000

guuaugggaa aaaggcgauu caucccuccc auuagugacc ugcgcaugaa ggcaaaacuu     3060

cagaucgagu ccauacugau cucuuucaaa aacaagaaua aagucgugac auggagcgug     3120

aacaagacca aauugaaggg uaaggauaau uacaacaaac ggagacagcu cacuccaagg     3180

ggccaacugc auaaggaaac agucuauggg aaaauuaagc uaaucgaccc uaaaccccug     3240

aaacuuaaua acaaguuuac gcucguucgu gccuccauga uuguggauaa agugcaccgc     3300

gagaucguac ugcagcacuu gucaaaguac gacaauaaca gcgaagucgc uuucgauagu     3360

aaaaccauaa agauugaccc aauccugauc gagggaauug gcuuaaaaga ggugguuugc     3420

uaugaagaug uguuuaagau acggaagaaa aucucuggug acaauuucaa gaacgagaaa     3480

caucucgaaa aggucauuga ugagaaagug aagcagaucc ugaaagacag aguuauuaag     3540

uuucagggga auuucaagga ggccuuuucc aaccuugaag agaaucccau cuggcugaac     3600

aaaagcaagg gcauagcagu gaaaucggua gcuaucaagg gagucaauaa cgccgaauca     3660

uugcacgagg gcaaagauca uuucggggac caaauucucg acaagaauaa gaaacagauc     3720

ccuguggauu uugugucuac uggaaacaac caccacgucg gcauuuacga agacaaggau     3780

gguaaucugc aggagagggu ugugaguuuc uuugaggcgg uaacacgagu gaacgaaggg     3840

cuaccaauca uagacaaaac cuauaauucc gauauuggau ggaaguucaa auucacaaug     3900

aagcaaaacg agauguuugu cuucccgagc gacgauuuug accccaauga guucgaucug     3960

acugacggca agaacgccgc uaaaaucucu ccuaaucuuu accgggugca gaagaucgca     4020

accaaaaacu acauguuucg ccaucaccuc gagacgguug ugaccaauga ucuggaguuc     4080

acauauaaga gcauuagauc cacugguugg uugaggaacu uagucaaagu gcggaucaau     4140

caucugggcg aaauaguuua cgugggggag uauuga                               4176


<210>  3243
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3243
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggaugggc ccuggugaac       60

gagaaggaaa auaacgagga auuguccucu auaaucagau uaggugucag gguuaauccc      120

cugacugugg augagaaauc aaacuucgaa aagggcaaag acauuacgac caaugcugau      180

cggacaaagu gccgcagugc acgacguaac cuacagagau uuaagcucag gcgggaggcc      240

cuggcggacu gucuuaaacg ccacgggcug augacugaug agaccccuuu gugcgaaaca      300

aauaacagca ucauuuucaa gaccuacucc aauagagcua aagccguaug ugagaagauc      360

gcacucgacg auuuugugag gguccuguuc augauaaaca aaaagcgggg auauaagucu      420

ucgcgcaaac ugaauacuga cgaugaaggc acacucgugg acgguaugga uguugccaag      480

uuacuguaca acgagcaaau caccccaggg gaguaucuuu ugcgacuguu uaaaaagggc      540

aaaaagacga gaccggacuu cuaccuauca gaucucaaag cugaacugga gaggauuugg      600

gacuuucagc ggacuuucua ucccgaaauc cuuugcaaug cacugaagga ggacuugaag      660

ggaaaaagcc guacccagag uuccgccauc uuuuacgcua aguauaaagu gucuacagcc      720

aagauugcgg gcaaagauaa gcgccuccag gaacugacuu ggagaaccga cgcauuaagg      780

aagcaacuga caauagauga ggucgccuac gugauuugua gcaucaacgg ggagguaaau      840

ggauccucac agcggcucag uaugauuagc gaccgaucua aacuucugua cuucaaccau      900

cagaccaucg gucaguauuu gaugcaacgc cuagaagaga acccuaaugc uggccugaag      960

aacaucauau uuuacagaca ggauuaugaa gacgaguuca augauauuug gaacucccag     1020

gccacguacc acaaagaacu cacuccagag cugaagaaag agguuaagga caucaucuuu     1080

uaucagaggg ggcuuaaguc augcaaaugg cugauuaauu acugugaauu ggagcaugaa     1140

gagauagaag ugaagacacc cgagggaaaa cuccggaaga uuaccaucgg cagcaaaacu     1200

ugcccuaaga guucuccacu guuccaagag uucaagaucu ggcaggucuu aaacaaucua     1260

caggugagcc cccugaacuc ccgcagaaaa gcaaagagga aaaaggauga ggaaccuucg     1320

cucuuuucug acgaagauau gaauacagcu ugucguuauc ugaacgagga ggaaaaacuu     1380

accuuguuca aggagcuguc cauuaaaaag gaacucaaga aaaaggacgc ccugaaacuu     1440

cuguuugaga auuacaagga auuggaccuc aacuacaaag ugaucucagg uaaugucacg     1500

auggcgaacc uguaucgggc cuuccaagau auuauaaguu uaaccgggaa uggagaguuu     1560

gacuuccagg caaugccagc ugaggaagcc gagaagacag uugaagagau cuuuggcggc     1620

cugggguaca agacugcuuu ccuacacuuu gaugccagcc uccaugacaa agaguucgaa     1680

cgacagccgg cauauagacu guggcaccuu uuguacuccu augccggaga ucagucuaag     1740

accgguauug aggcucuggu gaaaaggauc caagaacucu gcggcuuuga gcccgaauac     1800

gcgcgcaucc uggcaaacau uagcuucaua ccugacuaug ggaacuuauc aaguaaggcc     1860

augcggaaaa uccugccaua caugcaggau ggauacgagu auuccgaagc auguagcaag     1920

gccggcuaca gacacucugc aucaagucuu acaaaggagg agauuaauaa aaagaacuau     1980

aaaacucauu uggacgaaau caagaggaau agccuccgca accccauugu agagaaaauc     2040

cugaaucaga uggugaacgu cguuaauucc gugauagaua ccuacucuaa caucgacgaa     2100

auucgaaucg agauggcccg ggagcuaaag aagucgaaac cugaacguga ggauaugagc     2160

uuugcuauuu ccaaggccac aaaagaaacc gagagaauca aggguauacu ggaacaggag     2220

uucaaaauuc aacacgugac uaggaaugac aucauucgcu aucggcucua cgaggaacug     2280

aagccaaacg gguauaaaac gcuuuacuca aauacauaca uccccagaga ggauuuguuc     2340

aguaagaagu uugacauaga acauaucauu ccgaaagcaa ggcuguucga cgauucuuau     2400

agcaacaaga ccuuagagcu ccgagacauc aauauugaaa aaggcaacca gacugcguac     2460

gauuuugucc uguccaagua uggagaggag ggccuuuaca aauaugaaca gcgcguggag     2520

gaguucaaga agaauaacga acaggcuggg accaaaugcc ggaagcuguu gaugagagaa     2580

gccgacaucc cuucagauuu ucucaacagg gaccugaaug auagccaaua cauagcuaaa     2640

aaggcccacg agcuacugcg ugagauugua cccacaguua ccgcuacuac aggaucuauc     2700

acgaaccgcc uccgggaaga cuggaaacug gugaauguca ugaaggagcu uaacugggaa     2760

aaguaugaua aauugggucu gaccgaggag uacaaggaca aagauggcaa uacugugaga     2820

cacaucaagg acuggacaaa aaggaacgau caucgacacc augccaugga cgcauuaacc     2880

auugcuuuca caaagccauc cuuuauccag uaccucaaua accugaaugc ccggaguaac     2940

cgcgggggaa gcauuuaugg cauagaacag aaggagcuug aaagagagaa uaacaaacug     3000

gucuucaucc cucccauuga cccaaagauc uuuaggaaac aggcgaagga acaccuaucu     3060

aauauucuca ucuccauaaa agccaagaac aaggugguua cuaaaaaugu gaacaagacc     3120

aaaucaaaga gugguacgaa ccggaaagua caacugacac cccgagggca guugcaucug     3180

gagaccgucu acggcaagau cagaacuuau aaaaccgagg aaauuaaggu gggaacaaag     3240

uuaacugagg aacucaucgc uaaaguugca gauaagaaaa uucgcgaggc ccugaugcag     3300

aggcuuagcg aauacggcgg gaauaucaag aaggcuuucc aaaacauauc gaaacacccu     3360

auuuggcuga auggauccca caccaagcau gugccagaga aagucaagau cguguuuuuc     3420

gaggacguau uuacgauccg uaaagccauu acaccggaua uaaagaucga caaagugauu     3480

gauucuaaga uccggagaau uuugcaggaa aggcucaacg aguucggugg cgaagcaagc     3540

aaggcguucu caaaucugga cgagaacccc aucugguuua aucgcgauaa agggauagcc     3600

aucaagagug uuaaaauucg aggaaucgcu aagggcaccg ccauuagagu caaaaaggac     3660

auccacggua agacuauaau ugggaacaau ggcuauccug uggcaaacga uuacgugaau     3720

acaggaaaca aucaucacgu cgcuaucuuc cgggacaaag auggcaaccu acaggaacac     3780

aucguuuccu uuuaugaggc caccgcaagg gccauucaga aucugccaau cguggaccgc     3840

aacuacaauu cugagaagga cuggaaauuc cuuuuuagca ugaagcaaaa cgaauacuuc     3900

guguuuccca acccuaauac uggguucaac ccauccgaga uaucacucaa agaugaaaag     3960

aauuaugcuc ugauuagccc caacuuguuu cguguacaga aacugaguca uuacuauuac     4020

gucuucagac accauuuaga gaccaaugug gacacaaacc uccagcugaa ggauaucacg     4080

uggaagcgga uuaccgcggu uaauaaccuu acuggagccg ugaaagucag gaucgaccac     4140

cugggccgca uaguggaugu uggugaguau aaguga                               4176


<210>  3244
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3244
augaagaacu accacaucgg ccuggacauu gggaccagcu ccgugggauu cgccgcuaga       60

gaugacgaua auaaccucau aagggucaaa gguaagaaug uuaucggcgc acggcuuuuc      120

ucugaggggc agacagccca agaacgccga auggcgcgua cuucaagaag gcgguauaac      180

cgcagaaaau ggaggcugcg guuguuagac gagauuuucc augaugaaau cauucacaag      240

gacgcugagu uuuucaaucg ccugaaacag aguuuucuaa gccccaagga ugaacgaaag      300

caguacuucg gaucccuccu guuuccugag acgggcgacu cugaguauua ccauaacggu      360

aaucagacca ucuaucaccu uagacauagg cugauaacac aagaugaaaa agccgacauc      420

cgggagauuu acuuggcacu ccgucacauc auuaaguauc gcgggaacuu ccuggaugac      480

acuccagugu cgucauuuga agccagcgau cugaaacucg cuaguuucgu accgaccauc      540

aaugacuuau uugagacaau agacgugaac uacgaguucu ccauggauaa uauuaacgaa      600

aucgaggaca uucugcuuaa uaacaaguug agaaauucug auaaaagcaa gcagcugacc      660

gaccuacuca cugaacccau cgucaacauc gaugacaagu cccuggagaa acaguuuaac      720

aaggaaagga cagauauuuc aaaagagaua gcaaagagug ugguuggaag caaaaugaau      780

gugaacaaac uucugucuuu cgaguccuca gaaggcagca aaaucgccuu caguuuuucu      840

gacgcuucca gcgaggauaa gaugauggaa uugucggaca uucucgauga cucucggaug      900

aauauccuga acauauuaaa ggucauuuac ucccgagugc gccugaauca gaucaucccu      960

ucaggcaugg ggcucaguga gagcauggua gccgcguaug acucccacaa acaacagcuu     1020

aaggaucuga aaaccuugcu aaacguucug ccauucaaug aaagauacgc agugaagauu     1080

gccuaugcug ccuacaucgg aaacauugac aaacagaagu uuacgaaguc ugagauaaaa     1140

gcaaucgaua agcucaaaga aaagagcgcu auuaugaaug uccuggacuu caaaucaggu     1200

cauaucacua acgaggagcu uaguaaugug aucaaggccg ugauugucga uaaguaucug     1260

aacgacgaag auuuugagua ccacggcaua guugaaaaga ucaaucagga cauuuccaac     1320

gggagcuauu ugcucaagca aaggaauaac cagaacggaa auauccccca ucagcugcac     1380

caaauggagu uagaucagau uaucgagaac cagucuaaau acuaccacuu ccugggcgaa     1440

cuaaauccua acucaaaucg gaaccauaua gcgaaguaua aaaucaguga gcucguggcc     1500

uuucgcguac cauacuaugu ggguccccug auuaccccug aggagcagga gaagacaagc     1560

ggggcauccu ucgcuuggau gaagagaaag aaggacggag ccaucacucc auggaauuuu     1620

gaagagaaag ucgauaagga agagaccgcu ucuagguuca uuaaacgucu uacagugaag     1680

gacaccuacu ugcugaacga agauguucuc ccggacaauu cgcugcuuua ugagaaauuu     1740

aaggugcuga acgaguugaa ugucgugaag gccaacaaua aacggcucac gguagaagag     1800

aagcaaggcg cauucgacaa ccuguuuaaa aagaugaaaa cuguuacagc caagcgauua     1860

aagaauuacc uggugagcaa ccacaaccau aucaauuccg ucgaaauaac cggccuauca     1920

gauggggaca aauucaacaa uagucucucu acuuauauug auuucaagaa aaucuuugga     1980

gacaagauug auaaccccgg ucugcuugac gauuucgagg acaucauaga auggagcaca     2040

guguuugagg auagaaaaau cuucgaggaa aaggucagga aggacauuuc cuggaucuca     2100

gagagcgaaa uuaaaucugu gguuuccaag cgcuacagug gcugggggcg guugagcaaa     2160

aagcugcuca auugcaucac caacaaugag ggaagaaaca uaauugauga acugugggag     2220

gagucugaaa cguuuaugga ggcugugucc aaaccugaag uaaaggagca gaucgacggc     2280

aucaauucag auuucauuaa gaacaccgac aucgagagug ucuuagcgga cgcauacaca     2340

agcccacaga auaaaaaggc cauuagggaa acucacaaac uggugaagga uauacagaaa     2400

gcuaugggug ggcaagcccc cgcaucgauc uccauugagu uuacccgcaa cccucaggac     2460

aagggccagc uuacacgauc ucggcauagc gaaguuucac guaaauauaa ggagaucagu     2520

aaggaaauuu ccaaaacuuu gaaugaugag cucaagucua aaaagaacag cauggacgug     2580

gauaaauacu aucuguacuu caugcagaug ggaaucgacc uauaugaugg cuccucaaua     2640

gacaucgaug accugaauaa cuaccaaauu gaucacauca uuccacagag cuuuuauaag     2700

gacgacaguu ucgauaacag aguccucacc cacuacucua auaaccagaa gaaagggaau     2760

aacacacccc ugaauggacu uaacaucaau gacaagacca gggccauaug gaaccauuug     2820

cgcgaucugg guuuaauuac uaaaaagaaa uacaauggcc ucacgcugaa ggggauggac     2880

cagcuuucca aguauacaaa acacggauuu auccggagac aacuggugga gaccagccag     2940

guaaucaagu ugguggcuaa cauucucucg gaugaauuuu caacugacga uaccaaaaua     3000

aucgagguua aggccaaaau gaacucucag auuaggcaag ucuuuaaucu guacaaggug     3060

cgagaacuaa augacuauca ucacgcagug gaugcguacc ugacaaccuu caucggcggu     3120

uaucucuaca accgcuaucc gaaucugcgg aacauguucg ucuacgggga cuuuaagaaa     3180

uucagugcug auguugacaa ucuuaagacu uuuaacuucu ugcacgagau uacaaacccu     3240

uccgauaaau uugaagacag aaucuucgac gaaaagagcg gcgagcugau auuagauagg     3300

aaacaugcca ucgaaacgau uaagaagauc uaccaguaua aauacaugcu cauuacccgu     3360

gaggugucua cuaagaaugg acagcugagc gaccagacag uguauuccgc aaaacgcauc     3420

aacaauguaa aggcccccau accagucaaa aaggauuuua acccugaacu uuacggcuuc     3480

cggaccggga auguggacca ccauaugguu auugugaaau aucccaagaa ggauucauac     3540

gacuauaaag ucgugggaau cccacugaga uuuguucaaa agcuaaaaga gacaaaggau     3600

aacaaagacu acgaaaaucu ccugaaccag aucgugcaga ugaaguugaa uaggaagaaa     3660

caggauuuca uuguagucgu ggacaacgug auguacgguc aacugaucca ggauggcggg     3720

cagaaguuua cuuuaggaag uagcaccuau cgauacaaug cuaaacagcu cgucauagac     3780

aacggcuauc ugaaacuuau ugccgaugag cgguacaaaa auucucugac ggacgaggaa     3840

aaguccaacc aauauaucac cuuguacaau gagauucuca agguugugaa cggucgcuuc     3900

gacguauucg augugaacuc auuuaggaaa aagcugaaua acggggucga caaauucauc     3960

aagcuaaaua caguggauaa aagaauaauu aucaugaaga uccugagugg ccuucacugu     4020

ggacccagca uuguuccucu caaggaacug ggcuugacua ccccacuggg gcaguuacag     4080

uccacaucug gaaucucgcu cagcgagaac acuauuauaa ucuaucaauc accguccggc     4140

cuguuugagc ggaaagugcg acuuggugac cuguga                               4176


<210>  3245
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3245
augaagaacu accacaucgg ccuggacauu gggaccagcu ccgugggauu cgccgcuaga       60

gaugacgaua auaaccucau aagggucaaa gguaagaaug uuaucggcgc acggcuguuu      120

ucugagggga aaacagccca ggaacgccga auuucacgua cuucaagaag gcgguauaac      180

cgcagaaagu ggaggcuucg guuguuagac gagaucuucc augaugcgau ugugcacaaa      240

gacgcugaau uuuucaaucg ccugaagcaa agcucccuau cucccaagga ugagcgaaaa      300

caguacuuug gaucgcuccu guucccugaa guaggcgacg gugauuauuu uagagacggg      360

aaccagacga ucuaccaucu uaggcaccgg cugaugaccc aggaugagaa ggccgacaua      420

cgugaggugu auuuggcacu ccgccauauc auuaaauaca gaggaaauuu ccuggaugac      480

acaccaguca guagcuuuga agccagugac cugaagcucg cuuccuucgu gccgacuuua      540

aacgaucugu uugagaccau caauguuaac uaugaacuuu cuauugacaa ugugaaccaa      600

aucgagaaua uauugcugaa caauaaacua aggaacagcg auaaguccaa gcagcucaca      660

gaccugauug cagagcccgu caccaacauc gaugacaaau cacuuaagaa acaguucgau      720

aaggaacgga aagacauuag uaaggagauc gccaaagcug ugguaggcag caagaugaau      780

guuaacaagc uguugucuuu ugaauccuca gauggcagca aaaucaguuu cucuuucucc      840

gacgccagcu cggaggauaa gaugcucgaa cugucugaca uuuuagacga uucccgaaug      900

aauauacuga acgugcucaa agucaucuac ucacgcguga gacuuaauca gauuaucccu      960

agugggaugg gacugagcga guccaugaua gcggcauacg acuugcacaa gcaacagcua     1020

aaagaucuga agacucuccu ggacgugguc ccauuuaacg agaaguaugc cguuaaaauu     1080

gcuuacgccg cauauaucgg uaauaucgau aagcagaggu ucacaaaagc ugaagugaag     1140

accauugaca aacuucggga uaagaagucu aucacgagcg uacuggacuu uaaaucaggc     1200

cacauuacua acgaggaauu gaauaacgug auaaaggagg ucaucgugga uaaauaccuc     1260

aaugacgaaa ccuucgagua ucaugauauu guugagaaga ucaaccagga caucaguaau     1320

gggaacuacc uguuaaaaca acgcaauaac cagaacggaa auauucccca ccagcugcau     1380

caaauggaac uagaucagau aaucgagaag caguccaagu auuacccuuu ucucggcaaa     1440

cugaacccaa auagcaagag aucucacauu gccaaauaca agaucucaga acuuguggcg     1500

uucagggucc ccuauuacgu ggguccuuug auuacaccag aggaacagga gaaaaccagu     1560

ggggccagcu uugcauggau gaagcguaaa gaggacggag cuaucacccc guggaacuuc     1620

gaaaagaugg uagacaagga guccacagcc ucucgguuua uaaaacgacu gaccguuaag     1680

gauacguauc uccugaauga agacgugcuu cccgauaacu cgcuguugua cgagaaauuc     1740

aagguccuca augagcugaa cguggucaaa gcuaauaaca agagauuaac uguggaagag     1800

aagcaaggcg ccuuugacaa ucuguucaaa cagaugaaga caguuaccgc aaaaaggcua     1860

aagaacuauc ucgugagcaa ucacaaccau aucaauuccg uaaaaauuac uggccuguca     1920

gauggggaaa aguuuaacaa cagucuuucu gucuacaucg acuucaagaa aauuuucgga     1980

gauaagaucg acgauagcaa auuguuugac gauuucgaga auauaauuga augguccaca     2040

guguuugagg accgcgauau cuucgaggaa aagguucgga aagacauuuc auggaucagc     2100

gagucugaaa uaaacgccgu ggucuccaag agauauagug guuggggcag gcugagcaag     2160

aaacuccuga augggaucac caacgacgag ggacgcaaua uuaucgauga auuaugggag     2220

gagucugaaa cguuuaugga ggcugugucc aagccugaag uaaaagagaa gauugacgaa     2280

aucaacucaa auuucauaaa gaacaccgau auugagagug ugcuggcgga cgcauacaca     2340

agcccacaga auaagaaagc cauccgagag acuuacaaac uuguuaagga uauccagaaa     2400

gcuaugggcg gucaagcccc cucguccauu ucuaucgaau uuacccggaa cccucaggac     2460

aagggggagu ugacacguag cagacacuca gaaauugcca aaaaguauaa agagcucagu     2520

aaggaggucu ccaagaaacu gaaugaugaa cuaaagucua aaaagaacag cauggacgug     2580

gauaaauacu aucuguacuu caugcagaug ggcauagacc ucuaugaugg aacuuccauc     2640

gacauugacg aucugaauaa cuaccagauc gaccauauug ugccacaauc auuuuauaag     2700

gaugacagcu ucgauaauag gguccuuacc cacuacagua acaaccagaa gaaaggcaau     2760

aacacacccu ugaaugggcu gaacaucgac ucuaauaccc gcgagguuug gaaccauuua     2820

auaggacucg gucugaucac uaagcggaaa uacaauaacc uuacgcugaa gggcauggau     2880

caguugucca aauauacaaa gcacggguuu auuagaaggc agcucgugga aaccagccaa     2940

gugaucaagc ugguagcaaa uauucuaucg gacgaguacu caaaagauga cacuaagauc     3000

auagaaguca aagcuaagcu gaacucucag auucgacagg uguucaaucu cuauaaaauc     3060

cgcgagcuga acgauuacca ccaugcccuu gacgcauauu ugaccacauu uaucggaggc     3120

uaccuguaua aucgguaccc gaacuuaaga aauauguucg uuuacgguga uuucaagaag     3180

uuuagugcga aaauugacaa ccucgagacc uucaauuuuc ugcacgacau aacuaacccu     3240

uccgauaagu ucgaagacag gaucauugau gagaaaagcg gggaagugau ccuugaccgu     3300

cgccaugcua uugagacaau caagaaaaua uaucaauaca acuauaugcu gaucacgcgg     3360

gaagucucua ccaagaaugg ccagcuaagc gaucagacug uguacuccgc cagaaggauu     3420

aacaaaguua auucacccau cccagugaag aaggacagua acccugagcu cuauggauuu     3480

cgaacaggca auguagauca ccacaugguc auugugaaau accugaacaa ggacaaauau     3540

gauuacaaga ucauagggau ucccuugcgg uucgugcagg uccugaaaaa uagcaacgac     3600

aauaccgagu acaagaacau cuuaaauaag aucguucaau cuaaacucaa gggaaaaucc     3660

acagauuuuc gcgugauucu ggacaacgua auguaugguc agcuuaucca ggauggcggg     3720

cagaaguuca cucugggauc aaguaccuac aaauauaaug caaagcaauu ggugauaaac     3780

auggacgaca uuagaauccu cgucgauaau agguacuuua acagccuguc cgacgaugac     3840

gccucuggcg aguucauuaa gaucuauaac gaaauacuaa aagugauuaa ugcucgguuu     3900

gaucuguucg acaucaacaa guuccgagaa aaacuuaaua acgguaagga guacuuugaa     3960

aaucucucga ucgaugagaa aaaccagguu cuguugaaga ugcugagcgg guuacauugc     4020

ggcucagcca augugccaau uaaggagcuc ggacugacga cuccccuugg caaacugcag     4080

aucccuuccg ggauugucuu gagugaagac gcaauaguga ucuaucaguc uccaagcgga     4140

cucuucgaga gaaagauucg ccugaaagau aucuga                               4176


<210>  3246
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3246
augaaccuga gaaauuaccc cggcgaguau agcaucgggc ucgacauggg aaccgguucc       60

gugggcuggu cugucguuga ugaaaagggg aaccuucugc acuucaaaaa gaaaccuaca      120

uggggaucaa gguuguuuga cagugugaug ccagccagcg aggcucgggu acaucgcggc      180

cagcgacgua gauacgaaag gcggcgcugg agauuagauc ugcuacaagg ucucuucaag      240

aaugagauga acaaagugga cccggaauuu uucaugaggc ugaaucaguc ccggacuauu      300

gagggggauc ccauauuuac gaagucuuuc accaaggcag acuauuacga ucgcuuuccu      360

acaaucuauc accuucgagc cuaccugaug gagucgaacg aaccagcgga cuugagacuc      420

auuuaucugg cuaugcauaa uaucauuaaa cacaggggaa acuuccugcg ggaggaagag      480

aagucacuca gcgccaaaca ugcagauagu aagaaagccg ucacucaguu uguggacgcu      540

uuaaaggagu ggugcuucuc ccguggcuac gaauuuggca aggaucccgc aaaaucuauc      600

gagaaguguc ugagcgacuc caaaucaagu cgcagccagc uugccucuga aauagcuaag      660

gccuuggcgg uuuccaccga ugcagccgcu gccgacaaaa agcuggcaga caaucuagcu      720

aaagccaugg uggggcucca augcgaguuc aaguacgucu uuggagaauu ugauugugag      780

ucaacaaagc uggcgcuuag cgacgaugag aaacuggaau ugcucagagc cgcaugcccu      840

gacgauagug uggagcuguu agacgcucug ugugccguau auucugcuua cguucuccag      900

ggucuucugu ccuaugcccc aggccagacc aucagccaca acaugauugc aaaguacgau      960

uuguauaaaa cugaccuaaa gacacugagg gcccucgugc gggaauacgc ucccggggcg     1020

uaugaugagu ucuuucgagg agcaaccuac cacgacgcca auccugauga cucgucuaaa     1080

gacuacuccu augcugaugc cgaaggcuac acggcauaua accugcauaa guugucauac     1140

gacgauuucg ccaaggcugu caaaaagcug uuggguggga cugacgccgu gaccgaugag     1200

cgcuaucuca auaugaugga gagauuugaa aaacagcaau uccugaggcg guuaaagaca     1260

agugacaacg gagcaaucua cuaucagcug caccuagagg aacucacugc gauuaucaaa     1320

aaucagggcc gcuuuuacag cuuccugaag gaguacgaag gcaaguuuga gucccuugug     1380

ucuuucagaa uaccauauua cgucgggccg uugaccagcg uuaacgcugc ccaggauuca     1440

cauaaccaca auagguuuca auggagugag cguaaacccg gaauggaacg ggaggugauu     1500

acaccuugga acugggacac caucauugau aagaauaaau ccgcagaaaa cuucaucaug     1560

cgaaugacgg gugacugcac uuaucuggcc ggcgagaagg uacucccaaa acagagccug     1620

cuuuacgagg aauuuugugu gcugaaugag uugaacgggg ucagaugguc ugaugacgga     1680

gaugacuggc agagguucga cgcugcccaa cgcgaaggca ucgugcggga gcucuuucac     1740

aaguauagaa ggguuacaua caagaugcug gaagauuggu uagugcgcga gggugacgca     1800

aaucauccca uugucaaagg gggccaggga gagaccggcc uggaaucaaa gauggggagu     1860

uaucuauucu ucgcuaaagg aauauuuggu cucggcgagc ugagcccugc cgaauaccca     1920

gcgaucgaga agaugauuaa auggaacacu cuuuucgaag aucgauccau cuugaaggag     1980

aaacuggagg acgaauuugg ggagcgggga aauggcaugc ucgaugccuc ucagauaaag     2040

gcuauuugca agaaacguuu cacagguugg gggagacugu cggaacaguu uuuaaccggc     2100

cugaaggugg agagccccgc aggaagggcc guauccauca uggacguucu ucgcgaaggc     2160

aacccguguu cagagcggag acaaggggag acggugguca ugauggaagc ucuuagggau     2220

gagggacucg guuuccagaa aaagaucgac gaacacaauc gaaacuacua uggccagcau     2280

gggaaagauc ugggagugaa ugagcuaccu ggcagucccg ccauucgccg gucucugaac     2340

caggcaauca gaauugucga ggaaauagcg agcaucgccg gucacgcucc agccaauauu     2400

uuuaucgagg ugacccgugg ggaagacgca ggcaagaagg gaaaaaggac aguuaagcgc     2460

ugggauaaaa ucggcgaggc ucucaagaaa cugaaggaag aggggguggc cguagagguc     2520

auggacgauu ucaaggaaca aaaagcaauu aacgccaacc uugacgagcg guugucccug     2580

uacuuaaugc agaauggaaa gugccucuau ucaggcaaag cuauaaaccu ggaaaaacuu     2640

cuggcgggua gcggggagua cgaaguggac cauaucauuc cuagagccua uaucaaggau     2700

gacucuauug agaauaaggc auugguucuc agggaggaaa accagcacaa gacugaucag     2760

cugcuaaucg acaaauccau acgauggaag augcgggaga ccuggaguca acugcacgau     2820

gcuggacuca ucggcgacaa aaaguaccau aaucugcuuc gcagcaacau uaaugaaaac     2880

caguugagag guuuuaucgc caggcagcug guggagacau cucagauggu caaauuagcc     2940

caagcucucc uggaagcacg guucgccaau gaggagacuc gaauugugcc cguaaaggcg     3000

uccaugucac acaaccuuag agaagcugcc gaucuggcaa aaugucgcga ggccaaugac     3060

uuucaucacg cucacgaugc auuccuagcc aguaggaucg gccucuuugu gcagaugcgu     3120

cauccaggga uauaugaaaa ccccauugga uacagccacg uuaucaagaa guacguccag     3180

gagcaaucgg agguguucaa aaagacccau cggaugccug gcuccgcggg guucgugauu     3240

aauucuuuua ugagcucagg auucgacaaa gauacaggcg aagucuuuaa ggacaguugg     3300

gacgcugagg ccgaaguuga ugguaucaga aaagcacuga acuauaggca gugcuacaua     3360

ucccgcaugc cacagcagga caccggggcc uucuggaaug cuacugugua uucuccgcga     3420

gaucccaaga ugggaccuaa guuggcccug agcacgaaac aaggcuuaga cccaaagcuc     3480

uacgguggcu uuuccucaca gcaguucgca uauuucuuca ucuacgaggc uaaaaagaaa     3540

gggaagccca cauuuagauu cagccaggug ccuguauggc uggccgcgcg gauugaagcc     3600

aacccacuug cacuggagga guaugcuagg ggauuggcca agggcgaagg gcucgaguuu     3660

gucaguaucc aacgccccaa aauuuacaag aaacagcuga ucgaagugaa cggagauaag     3720

cuaauaauua ccggcgcuaa agagguucgu ucuggugcag aacuggccuu caaucaggac     3780

gagaucuccg ccaucagcau gugcucgaaa cuuagagcug cggaggcagg gcucgaugga     3840

gccuucucag aagagguguc ugcuauuugg aaccggauca guaaugcccc gguccagucc     3900

uguggcaggc uguugcgcca acugacuuua ccuaaguacc agagcaacuu ugacucuagc     3960

uccaaugaag agaaggcagc cgugguucuc ggucugauug cgcuucugaa cggcaauaac     4020

cgaauagugg auuugucagg gguaggaggc aguagccacg cuggggccau gaugaugucu     4080

uauccaaagc uccuggacga ucccaaaguc ccuuucuuua ucauugacca guccgugacc     4140

ggaauguucg aaagaaagac acgggugggc cuauga                               4176


<210>  3247
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3247
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggaugggc ccuggugaac       60

gagaaggaaa auaacgagga acaguccucu auaaucagau ugggugucag gguuaauccc      120

uuaacugugg augagaaauc aaacuucgaa aagggcaaag acauuacgac caaugcugau      180

cggacaaagu gccgcagugc acgacguaac cugcaaagau uuaagcuaag gcgggaggcc      240

cucgcggacu gucugaaacg ccacgggcuu augacugaug agaccccucu gugcgaaaca      300

aauaacagca ucauuuucaa gaccuacucc aauagagcua aagccguaug ugagaagauc      360

gcauuggacg auuuugugag gguccucuuc augauaaaca aaaagcgggg auauaagucu      420

ucgcgcaaac ugaauacuga cgaugaaggc acacuggugg acgguaugga uguugccaag      480

cucuuauaca acgagcagau caccccaggg gaguaucugc uucgauuguu uaaaaaggga      540

aagaagacga gaccggacuu cuaccuguca gaucuaaaag cugaacucga gaggauuugg      600

gacuuucagc ggacuuucua ucccgaaauc cugugcgacg cacuuaagga ggaucugaag      660

ggcaaaagcc guacccagag uuccgccaua uuuuacgcua aguauaaagu gucuacagcc      720

aagaucgcgg gcaaagacaa gcgcuugcaa gaacucacuu ggagaaccga ugcacugagg      780

aagcaguuaa caauagacga ggucgccuac gugauuugua gcaucaaugg ggagauuaac      840

ggauccucac agcggcugag uaugaucagc gaucgaucua aacuccuuua cuucaaucau      900

cagaccaucg gucaauaucu gaugcagcgc uuggaagaga acccuaacgc uggccuaaag      960

aauauuauau uuuacagaca ggacuaugaa gaugaguuca acgacaucug gaacucccag     1020

gccacguacc acaaagaacu gacuccagag cucaagaaag agcugaagga uauuaucuuu     1080

uaucaaaggg ggcuuaaguc augcaaaugg cugauaaauu acugugaauu ggagcaugaa     1140

gagauugaag uaaagacacc cgagggaaaa cuccggaaga ucaccaucgg cagcaaaacu     1200

ugcccuaaga guucuccacu guuccaggag uucaagauuu ggcagguuuu aaauaaccug     1260

caagugagcc cccuaaauuc ccgcagaaaa gcaaagagga aaaaggacga ggaaccuucg     1320

cucuuuucug augaagacau gaacacagcu ugucguuauc ugaaugagga ggaaaaacuu     1380

accuuguuca aggagcuguc caucaaaaag gaacucaaga aaaaggacgc ccugaaacuu     1440

cuguuugaga acuacaagga auuggaucuc aauuacaaag ucauuucagg uaacgugacg     1500

auggcgaguc uguaucgggc cuuccaggac auaaucagcu uaaccgggaa uggagaguuu     1560

gauuuccagg caaugccagc ugaggaagcc gagaagacag uggaagagau uuuuggcggc     1620

cugggguaca agacugcuuu ccuacacuuu gacgccuccc uccauggaaa agaguucgaa     1680

cgacagccgg cauauagacu guggcaccuu uuguacucuu augccgguga ucaaagcaag     1740

accggcaucg aggcucuggu caaaaggauc caggaacucu gcggguuuga gcccgaauac     1800

gcgcgcauuc uggcaaacau aucauucauc ccugacuaug gaaauuuaag uuccaaggcc     1860

augcggaaaa uucugccaua caugcaggau ggcuacgagu auagcgaagc auguucuaag     1920

gccgguuaca gacacucagc aaguagccuu acaaaggagg agaucaacaa aaagacuuau     1980

aaaacccauu uggacgaaau uaagaggaac ucccuccgca aucccaucgu ugagaaaaua     2040

cugaaccaga uggugaaugu agugaacucu gucaucgaua cauacggguc gauugacgaa     2100

auccgaauug agauggcccg ggagcuaaag aaaagcaaac cugaacguga ggauaugucc     2160

uuugcuauca auaaggccac caaagaaacu gagagaauaa agggcauucu ggaacaagag     2220

uucaaaaucc agcacgugac gaggaacgac auuauccgcu aucggcucua cgaggaacug     2280

aagccaaaug gauauaaaac acuuuacuca aacaccuaca uacccagaga ggauuuguuc     2340

aguaagaagu uugacaucga acauauuauc ccgaaagcaa ggcuguucga cgauucuuau     2400

agcaauaaga cuuuagagcu ccgagacauu aacaucgaaa aaggcaauca gaccgcguac     2460

gauuuuguuc uguccaagua uggggaggag ggacuuuaca aauaugaaca gcgcguggag     2520

gaguucaaga agaacaauga acaagcuggu ucaaaaugcc ggaagcuguu gaugagagaa     2580

gccgacauac cuagcgauuu ucucaacagg gaccugaaug auucucagua cauugcuaaa     2640

aaggcccacg agcuacugcg ugagaucguc cccacaguga ccgcuacuac aggcuccauc     2700

acgaaccgcc uccgggaaga cuggaaacug guaaacguua ugaaggagcu uaauugggaa     2760

aaguaugaua aauuggggcu gaccgaggag uacaaggaca aagauggaaa cacugugaga     2820

cacauuaagg acuggacaaa aaggaaugau caucgacacc augccaugga cgcauuaacc     2880

aucgcuuuca caaagccaag uuuuauucag uaccucaaca aucugaacgc ccggagcaau     2940

aagggcgguu cuauauaugg gaucgaacag aaagagcuug aacgcgagaa caauaagcug     3000

gucuucauuc cucccaucga cccaaaaauu uuuagaaagc aagcgaaaga acaccuaucc     3060

aacauccuca uaucaaucaa ggccaagaau aaagugguca cuaagaacgu gaauaaaacc     3120

aagaguaaaa gcggcacgaa caggaagguu cagcugacac cccggggaca guugcaucug     3180

gagaccgugu acggcaaaau ucgaacuuau aagaccgagg aaaucaaggu agggacaaaa     3240

uuaacugagg aacucauugc uaaggucgca gauaaaaaga ucagagaggc ccugaugcaa     3300

cgccuuucgg aauacggagg uaauauuaaa aaggcuuucc agaacauuuc caagcacccu     3360

aucuggcuga acucuagcca caccaaacau gugccagaga agguuaaaau cguguuuuuc     3420

gaggacgucu uuacgauuag gaaggccaua acaccggaua ucaaaauuga caaggugauc     3480

aauucaaaga uucguaacau cuugcaggaa cggcucaaug aguucggcgg ggaagcagcg     3540

aaagccuuca guaaccugga ucagaauccc auaugguuua acaaggacaa aggaaucgcu     3600

auuaaguccg uaaaaaucag aggcauugcc aaugguaccg caaucagggu gaagaaggau     3660

auacacggga acacuauuau cggcaauaac ggauauccua ucccaaauga cuacguuaac     3720

acaggcaaua accaucacgu cgcuauuuuc cgcgaucgag acgggaaucu acaagagcac     3780

aucgugucuu uuuaugaagc caccgcaaga gccauacaga accugcccau uguggaucgg     3840

aauuacaaca gcgagaaaga cugggaguuc cucuuuucca ugaagcagaa cgaauacuuc     3900

gucuuuccua auccaaacac uggauucaau ccccuugaga ucgaccugag gaacgaagag     3960

aauuaugcuu ugauuucacc gaaccuguuu cgcguucaga aauuaagcca uuacuauuac     4020

guguuccguc accaucucga gaccaaugug gauacaaacc ugcaacuuaa ggacaucacg     4080

uggaaaagaa uaaaggcgau uaauaaccug accggcguag ucaaagugcg gaucgaucac     4140

uuggguagga ucguugacgu gggggaauau aaguga                               4176


<210>  3248
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3248
augaagaaaa gaaucgacga uuacauucug ggccucgaca uagggaccaa cagcugcgga       60

ugggccguga cagauaagaa aaauaaccuu cugaaguuga gggguaaaac ugcuaucggc      120

ucccacuuau ucgaggaagg gcauacggca gccgaccggc gcggauuucg aaccacacgu      180

agaaggcuga agcggcgcaa guggagacua aggcuccugg aggaaauuuu cgcggagccc      240

auggcugaag ucgauccugg cuuuuucguu cggcuucacc agucuugggu gucaccacug      300

gacaaaaguc gcaagaaaua ucaagccauc guauuuccga cugcaaagga ggaucaggag      360

uacuaucaga aauaccccac cauuuaucau uugcgagacg cccucaugac acaggauaga      420

caauucgacc ugagggaaau cuuucuggcu auacaccaua ucgugaagua ccgggguaau      480

uuccuccagg auaccgcagu caaggacuuu gaggccagca aaauugaagu ggggccuauc      540

uuaacuaaca uuaauaacgc uuucgccgau aagaucguug agggacagga ccugauagag      600

cuuaaugugg cgaacgcagc cgaaauugag gcugucaucc guggcaaaga cgccgauaag      660

acauuguaua aaauggacaa ggugaaaaag auugcaaagc ugcuaaccga uuccacggcu      720

aaagaacaga agaauguagc caaacaaauc gcgaacgcca ucaugggcua caagacucag      780

cucgagacca uucuggggaa agaaauagac aagaaugaua aggcacagug ggaguuuaaa      840

cuuucugacg cugaugacaa gcuggaugcc uugcucccag accuggauga gucggaccag      900

acaguugugg cugaaaucga gaaauuauuc ucagccauua cucugagcac caucgucgac      960

gaaaacaaga gucucuccca aucuauggug gagaaauaca auaagcacca ccaggauuau     1020

aaacuucuga aagcauacau agagagcuug cccgaccgcg cccaggcuaa gaaacuacug     1080

cucgcguaug aucuguacgu gaacaauaga cauggacacc uucuggaagc aaagacagag     1140

uuuaaacaag acuccaccuu gacgaaggaa gauuucuaua agacugucaa agacaaccuc     1200

gaugacucag agcuggccca uaagauugcu caggaaaucg ccgcagauaa cuucaugccu     1260

aaacagagga caaauaguaa cgguguuauc ccauuucagu uacaccaaau ugagcuggac     1320

aagaucauug ccaaucagag caaauacuau cccuuccuag cugccgaaaa cccuguggag     1380

gaucaccgga agcaggcacc auacaaacuc gacgagcugg uacgauuucg cgugccguac     1440

uaugucggcc ccaugauaac cgcggaugaa caggagaaga cuucugggaa guccuucgcu     1500

uggaugguga gaaaagaaca aggacagauc acaccuugga auuuugagca gaagguugac     1560

aggcaggagu cagccaacaa auucauuaag cggaugacca ucaaagacac guaccuuuug     1620

ggcgaagaug ugcugccagc aaauagccuc cuguaucaac gcuuugaggu ccuuaacgaa     1680

cugaauaaca ucagagugaa ugguaguagg auuucuguag acuugaagca gcagauauuc     1740

aacgaucucu uugaggaaaa gaaaaccguu acagagaagg cccugaucuc cuacuuaaaa     1800

cagaaucugc aucuacccac uguggagauu aaggggcucg cugacccuac caaauucaac     1860

agcucgcugg ccucuuauua ccaccuuaag aauuugcaug ucuuugauaa ggaacuggca     1920

gacccacaau aucagaaaga guucgaaaag aucauugagu acuccucaau cuuugaagau     1980

aaaaagauau uccaggacaa acuccacgcu gaguucaagu ggcugacacc cgcccaauuu     2040

aaggcgauca guacuuggcg uuuacaggga uggggccggc ugagccgaaa acuuuuggug     2100

gagcuccaug auaccaacgg ccagaacauu aucgaacagc ugugggacuc ccaaaagaau     2160

uucaugcaga uugucacaga gccggauuuu aaagaagcca ucgcuaagga gaaccagaau     2220

auaacccagg aaaacggggu ggaggagauu cuagcagacg ccuacacuuc uccugcuaau     2280

aaaaaggcca ucagacaagu ugugaaagua gucgcagaug ugguuaaggc ggccagcgga     2340

aaggcucccg cccaguucgc aauugaauuu acgagggacc cagacaaaaa cccucagcug     2400

ucacacaucc gcgguacaaa gcuccugaag gcuuaucagg agaccgccgg cgaacuugug     2460

gaucaaaauu ugacuaacag ucugaaggag gcaaugaccu cccggaaauu acucaaggac     2520

aaguacuucc uguauuuuau gcagacaggg agagaugccu acaccggcaa aaagauaaau     2580

aucgacgagg ucagcacuaa cuaucagauu gaucacaucc uuccccaauc uuucauuaaa     2640

gacgauucau uugacaauag ggugcugaca gcuacgccau ugaacgcgga aaagagugau     2700

gacguacccu acaaacgcuu cgccaauaac uaugugagcg aucucaagau gaccguugga     2760

gagaugugga agcauuggca gaaagcaggu aucauaaaua agcacaaacu ggggaaccua     2820

cugcucgacc cugaccgacu guccaaguuu cagaaaucug gcuucauuaa ccggcagcuu     2880

gucgaaacuu cgcaaaucgu gaaguuggug agcgucaucc ugcagaauaa guacccagau     2940

gcugagauua ucacaguuaa agccggagac aacuccgccu uacgucagag acucgaucug     3000

uacaagucaa gggacgugaa ugauuaucau cacgcuauug acgcauaccu uaguauagug     3060

uguggcaacu uucuguauca gguauacccg aaauaucgcc ccuacuucgu cuaugggcaa     3120

uucaagaaau uuucucagga uccugaccua cagaaggauc ucaucaagca cuucaaagga     3180

uuuaccuuca uguggccacu guugcagaag gacaauagcg aacggaaagc ccuggaggag     3240

auuaaggaaa acaauuccga uagaaucgug uuuuacaaac aucccgacau uuucgauaag     3300

uuaaggaaag cguacaacua uaaguacaug cucguuucac gagagacaag cacugaaucu     3360

uccaguaugu uuaauaugac caucuauccu cgcggugagc gggacagaaa gaaaacgagg     3420

aagcugauac caaaaggcaa cgggcuugac cccaagaucu acggaggcua uagcgguaau     3480

accgaugcuu acauggugau ugucaaaauc gaaaaggcca aggagucuau uuauaaagug     3540

aucgggauac cgaugcgugc acuggccggc uugaagcaag cuaaaaacca gggaaauuac     3600

aaggaggaac uccacaaagu uguggagccu cagauuaugc uggacaagaa cggcaagcca     3660

aaacgcgggg uagaaggauu ccgggucgug aaggaucaug ugcccuuuaa acaggucguu     3720

cuagacggug auaagaaauu caugcuguac uccgcaaagu augagacaaa ugccaagcaa     3780

cuuacucucu cacaggaaac caugagaauc gugacagaca accugaaaaa gagugaggau     3840

caggacaaau ugcugguaaa ggcguacgau gagaucuuac aaaaagugga ccaguaucuc     3900

ccucuguuug augucaacaa guucaggaau agccuucacc ugggccgagc uaaauucuug     3960

gaccucgccg ugaacgauaa gaagauuacu cugucgaaua uacuaaacgg gcugcaugac     4020

aaucuuguug ugcccaaccu caaaaauauc ggaauuaaga ccccauuugg ccugaugcag     4080

uccccuucug guaucacguu gagcucaaac gcaauuuuaa ucuuccagag ucccacaggg     4140

cuguuugaaa aacggguccg cauagccgac cucuga                               4176


<210>  3249
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3249
auggagaaca agcaguacua uaucggccug gacgugggga ccaauagcgu cggaugggcc       60

guuacagaua cuuccuacaa ccuccuuaga gcuaaaggua aggacaugug gggcgcaagg      120

cuguucgaug aagccaaaac ggcgguggag cggcgcacca agcgaacauc ucguagaagg      180

ucagaacggg agaaagcucg caaggccaug uugaaggaau uauuugcaga cgagauuaau      240

aaaguagauc ccaacuucuu uauaagacug gaggaaagua aguucuuucu agacgauagg      300

agcgagaaua accggcaacg cuauacucuc uucaaugacg ccaaauuuac cgauaaggac      360

uacuaugaaa aauacaagac aaucuuccau cugcgauccg cucuuauuaa cucugaugag      420

aaguuugacg ugagacuggu cuucuuggca auccucaauc uguuuucgca cagggggcac      480

uuccugaacg ccucacucaa aggagauggc gagauucagg guguggacau cuuuuauaau      540

gacuuauacg aaguuugcga ggaguucgag auagaucugc cucagaucag caacauugac      600

aauuucgaaa agauccuuag ucagaaaggg aagucccgga ccaaaauuuu ggaggagcug      660

ucugaagaac uaagcaucuc caagaaagaa aagauaaagu acucacuccu gaaacuuauu      720

uguggaauga agaucaaagu ggucgauauu uaugcugacg aggauaucca agaagacgau      780

aagaaaaagg accugggcuu ucgugagagu gauuacgaga aaagcucuuu ggaagugaaa      840

gagaucauug gcgacgauca uuucgaacuc auagagcgcg ccaaggcggu acacgacauc      900

gggcuguuag caaacauuau cggaaauucc aaauaucugu gcgaagccag aguugaggcu      960

uacgacaacc aucacaagga ucuccagaaa auaaaggagc uucugaagaa auaugacauu     1020

aaggccuaca cugauauguu uaggaaaaug acagacaaga acuauucagc auacgugggu     1080

agcgucaaua guaacaucgc uaaagaacgg cgaucugugg auaaucgcaa gaucgaggac     1140

uuguacgaau auauuaagaa aaucgcccua aagaacauuu ccgaugacaa ugaggauaaa     1200

auagagaucc ugaagaaaau ugaaaccggc gaguuccuca agaaacagcu gacggcgagc     1260

aacgggguga ucccaaauca gcuucaaucg agagaacuga gggccaucuu gaagaaggca     1320

gagaacuacc ucccguuucu gaaagaaaag ggagagaaaa auuuaacugu cucugagaug     1380

auuauacagc uguucgaauu ucagaucccc uauuacguug gcccucuaga caagaaccca     1440

aagaaggaua agaaugcuaa cuccugggcc gugauucccu caggugggcg gauccucccu     1500

uggaauuucg aggacaaagu agaugugaag ggaagucgca aagaauuuau ugagaagaug     1560

gucagaaaau guaccuaucu gagcgaagaa cacacacuuc caaagcaguc cuugcuguac     1620

gagaaguuca uggugcucaa cgaaaucaau aacauaaaaa ucgacggcga gaagauuucu     1680

guugaagcua aacaaaagau cuauaacgac cuguuuauga aaggcaagaa ggugagccag     1740

aaagccauua agcaggagcu uaucucacug aauauaauga guaaagauuc cgucuugagc     1800

gggauugaca cugugugcaa cgcauaccuc ucuucaaucg ccaaguucag gggaguauuu     1860

gaugaaaaug uuaacaauca aaguaccauu gacaugaucg aggauauaau cuuccugaaa     1920

acaguguaca gcgacgagaa gcguuucguc aaagaagaga uuguggaaaa guauggugau     1980

gagaucgaca aggauaaaau uaagcggauc uuaggcuuua aauucuccaa cugggggaau     2040

auaucuaagu cguuucugga gcuagaagga gcugacgucg gcaccgguga gauucgaagc     2100

aucauccagu cccucuggga aacgaacuuc aaucugaugg agcuuuuguc aaguagauuu     2160

acuuacaugg augaacugga gaaaagggug aagaagcucg agaaaccccu gucugaaugg     2220

acaauugagg acuuagauga cauguaucug agcuccccug uuaagcgcau gaucuggcag     2280

ucaaugaaaa uuguggacga aauacagacc guaaucgggu acgcgccaaa gcggauuuuc     2340

gucgagauga cuagaagcga aggcgagaaa gugaggacaa agucucgcaa ggaucgacuu     2400

aaagaguugu auuccgcaau caaggaagac aguaaacaau ggguuaagga gcucgaugcc     2460

aaagacgaaa gcuacuuucg gucuaagaag auguaucugu acuaccuaca gaaaggacgu     2520

uguauguauu ccggcgaggu gauugaacug gauaagcuca uggacgauaa ccuguacgac     2580

aucgaucaua uauauccgag aucauucguc aaagacgaua gucuugacaa uuuggugcug     2640

guaaagaaag agaucaacaa uaggaagcag aacgacccca uuaccccuca gauccaagcu     2700

agcugccagg gguuuuggaa gguguuacac gagcagggau ucauuucgaa ugaaaaauac     2760

ucccgccuca cgcggaagac ccaggaguuu ucugaugaag agaaacugag cuucaucaac     2820

agacaaauag uugagacagg ucaggccacu aaguguaugg cacagauucu ucagaaauca     2880

augggcgaag acgucgaugu gguguuuagu aaggccaggc uggucuccga guuccgacau     2940

aaauucgaau uguuuaaguc ucgccucauc aacgacuucc accacgcuaa ugaugccuau     3000

cugaacaucg uuguggggaa uagcuacuuu gugaaguuca cccggaaccc agcaaauuuu     3060

auuaaagacg cgagaaagaa ccccgauaau ccugcuuaua aauaccauau ggacagguuc     3120

uuugagcgug auguaaaguc caaaucagaa gucgccugga uaggacaaag cgagggcaac     3180

gcagguacaa ucgccauugu gaagaagacu augaguaaaa auucuccacu aaucaccaag     3240

aaaguugagg aagggcacgg cuccauuaca aaggagacca ucgugggagu caaagaaaua     3300

aaguucggcc gcaacaaggu ggagaaagcu gacaagacuc ccaaaaaggc caaucugcag     3360

gcauaccggc cgaucaaaac gagcgaugaa agacucugca acauucugag guauggggga     3420

cgaacaucga ucucaauuuc ugguuacugu cuuguugagu augugaagaa gcggaaaacc     3480

auccgcaguu uggaggcuau accuguauac cugggcagaa aggacuccuu aagcgaagag     3540

aaacuccuga auuauuuuag guacaaccuu aaugaugggg gaaaggacuc ugucagcgac     3600

auucggcugu gccuacccuu caucuccacu aacucacucg ugaaaaucga uggcuaucug     3660

uacuacuugg gugggaagaa cgacgaucga auccagcugu auaaugccua ccaauuaaaa     3720

augaagaagg aagaggugga auauauuaga aaaauagaga aggcggucag uaugagcaaa     3780

uuugacgaga uugaucgcga aaagaaccca guucucaccg aggagaaaaa uaucgagcug     3840

uacaacaaga uucaggacaa guucgagaau acaguguucu cuaaaaggau gucccuugua     3900

aaguauaaca aaaaggaucu gucauuuggc gacuucuuga aaaauaagaa gaguaaauuu     3960

gaagagaucg aucucgaaaa gcaguguaaa gugcuguaca acauaauuuu caaucuaagc     4020

aaccugaagg aggucgaccu uuccgauauc ggaggcucua aaucgaccgg gaagugccgu     4080

ugcaagaaaa auaucacuaa cuauaaggaa uuuaaacuca uucagcaaag caucacagga     4140

cuguacucau gugagaagga cuugaugacg auuuga                               4176


<210>  3250
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3250
augaagagaa acaaugacua cuauaucggc cuggauaugg ggaccaacag cgugggaugg       60

gccacaacua auccccaggg uaggcuccuu cgguucaaca aaaaggacau guggggcucc      120

cgccuguuug aggaagcuaa aacggcagcc aagacccgaa cauuccguac uucuagaagg      180

cgguacgauc gcgucagaca aaggauugag uuuuugcagg acauauucgc gccuaucauu      240

gcuaaaaagg auuuugaguu cuuuaugcgg uuaaaggagu caaaauucca ccuggaagac      300

aagaaaaccg augggaaguu ugcccuauuc aaugacgcag auuuuacaga cguugaguau      360

uucgaucgcu acccaaccau cuaucaucuc cgagccgcuc ugauuaaacc gaaccacauc      420

acugacccca gacuucugua cuuggcaaua caucacauca uuaagaauag gggacauuuu      480

cucuucgccg gcacagauau ggagaguauc cggagcuucc agauugugua uacccagcug      540

uccgaacacc ugaagucuau caaccucgac uuugaguuac cuguagacgc ucaagauaaa      600

uucgccgcga uacugcuuaa ugaaaaguug aacaaacgug acaagaaaaa gcagcugaaa      660

gagcuaugcc cacacaagga uaagcgccuc gaguacgcau uuaaugccau uaucgguggg      720

aaaacgaagc ugaaagacau uuucggagug gaagaguacg augaaggcac uaagaaagcu      780

gucgacuuug agaaggccga uuaugacgaa gauaaggacg agaucgaguc ggugaucaaa      840

gaaaacauuc auguucuuaa ugcacugaag gcuguguacg auuggaccau auugacaaac      900

auccucggcg acuaugccua cauuucagag agccaggucg cgcuguauga agcccacaaa      960

aaggacuuaa gaauccugaa aagucucaua aagaaguacg caaaauccca guaugcugau     1020

gccuucaggc augagaaugu gaaggggaac uacgcuaacu acguaggauc uacuaauuau     1080

gacaacaaac aacccguuau ugauaauccu agcgugaccc aggaagacau caacaaguac     1140

uuugccgaua uccuucuguc cauuacacca gacgagucag aucgggagag uuaucaguac     1200

aucaccgaac gauugcacaa aggugaggca cuacccaagc ugcgcacgaa ggccaauagc     1260

guccucccuc accagauuca uaaaauagaa cuggagaaga uccuugcuaa cgcggccaaa     1320

aauuucccau uucugcaaga aaaggacucu uccggcuaua cugcagcuca gaaaauugug     1380

aagaucauga uguucagaau cgaguacuau guggggccgu ugaacacaua ccaccauguc     1440

gaucagggag gccacgccug gaugguuagg aaagcagagg guaagauuac ccccuggaau     1500

uuugacgaaa agguggauaa agaggccuca gcugaaaagu ucauacggaa caugacuaau     1560

aaauguacau accucaucgg gaaggacgua auuccuaaac aaagccuguu auauagucgc     1620

uacaaggugc ugaacgagcu aaauaaccuc aaggucaaug gagccccaau cucuguggca     1680

cugaaacaga agauuuauga ggaacuuuuu aaaaagcagg uuaacgugac cuucaaccag     1740

uugaaaacgu uccugaagac ccaaggcgag gaugcggacg aaaucauacu cuccggcguc     1800

gacgaggcug cccauaaguu uacaaauaac cugcagacuu accacaaauu cagagcaauc     1860

cuuagggaua agauugaauu ugagccccag cguagcaugg uggaggacau cauucggugg     1920

aaaugccuga ugggggauga caucaccaug uggaagcaca aaauagaaaa ggaguaugga     1980

gaucaguugu cggaagaaca acucaagaaa auuacacgac ugaauuucaa cgguuggucu     2040

agauuauccc aggaauuucu gacugacgua ccuggcguug auaucgaguc aggggaggug     2100

uacaguggaa uuauggacgc ccuauggagg accaaugaaa acaucaugca gcuccugagc     2160

ucccgcuuca cauuugcugc cgaggucaag aaacagaauc aaggcaccgg ucggauagaa     2220

aagaucacuu augaugaggu gaugaaagac acguaccuuu cuccagcagu caagagaagc     2280

guguggcagg cuauuacauu gguugaagag aucaggaaaa uucgcaaggc cgaucccaag     2340

cgaaucuucg uggagaugac ccggcagccg gacgcgaaaa aggaacguaa acugucaaga     2400

aggacucagc uccugaacgc cuaccgcgcu auagggaaug auauugcaga guaugaaaag     2460

gagcauguaa guaccuuaau cacacaacug gaguccaaaa aggacgaaga ccuucggaga     2520

aagaaauugu accucuauuu uacccagaug ggaaggugua uguacacugg cgagcgaauc     2580

gaucugagcu cucuacugcg cgacacaaac ucaaauauuu augauaucga ccacauuuac     2640

ccucggaguc ucacgaagga ugacagccug gauaacaccg uccuuugcaa aaaggaaaua     2700

aauuugaaaa agggcaacaa guauauccau agaguguuca cugacaaaga gcugaagaaa     2760

caguacgggu acuggaaggc cuuaaaagaa aagaagcuca uuuccgagag gaaauuuucu     2820

cgucugaugc gcacaaccga ucuuacagcu gacgagcugg ccggauucau cucgcggcaa     2880

uugguugaaa cuagccagau ggugaaggca accgcggaua uucucgagaa aaucuauccc     2940

aaugucacga uaguguacgu aaaggccaga accguguccg acuuuaggca cgacuaugaa     3000

cugccaaaag uucgagcucu aaacgauugu caccaugccc acgacgcaua ccugaacauc     3060

gucgugggua auguguauua cgagaaguuc acaaagucac cuuuccgguu uauugcuaac     3120

cagaaaggca agggggauau caaauauaau cucuaccgcc uguucgacaa ggaugucaaa     3180

guuggagaca gaauuaucug gaacgccaag gaagaucuug caagggugaa agccacuuug     3240

gagcgggaca ccgugaaugu aguccgaaug cccguggagc agagaggcca auuuuucgau     3300

gcuacaauac agaagaaggg uacuaccaaa aacaugcugg uuuuaccacu caaggggagu     3360

gacgaacgcc ugcaggauac gucuaaauac ggcuauuacg ugaggcccag cggaaugucc     3420

uuuauggucg ugcagcauga gguuaagggc aaaucacgug ugacaauuga accugcgcuu     3480

cugguauaua agaauaagcu aaaaagcgag gacgauuacg ccaaguauug cgaagacguc     3540

cucaaacugc aaaagccaaa aguguuguuc aagaacauca aguuuggguc uaaaauccug     3600

uucgacggau acccguuuuu acucaagggu cggaccgaga auagaauuau aauggcagau     3660

gcucaguccc uguuccuugg caaccaggag cugaaaaucu ugaagagggu gacuaaaauu     3720

agugacgaua ucgaccacaa ggucgaaagc cgcauuaaga ucacagauga ggaauggacc     3780

acucucugga aaucuguugu ggagcagaua guaaccggga aguuucgaaa uagacuggga     3840

acacuaaacg gcauccugca aggucggaaa auuaagcugu ccaaagacga ggaagccagg     3900

cucaaugagc uggauucaaa ggaccaggaa acguucuuga gucagauccu guuagagcgc     3960

uuccuccaau uuagcaccga agagaaaacu cgucugcuuc uucaggugau uucguucucc     4020

gggaacacaa ccggcgucga uuugagacuc aucggaggcu cuagcgggac aggaacuaua     4080

aagaccaaua agaacauuac ggaccauuca gugauccuga ucaacgagag ucccacaggc     4140

cuauuuacca aucggacugu ucugaaccag ccuuga                               4176


<210>  3251
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3251
augaagagaa acaaugacua cuauaucggc cuggauaugg ggaccaacag cgugggaugg       60

gccacaacua auccccaggg uaggcuccuu cgguucaaca aaaaggacau guggggcucc      120

cgccuguuug aggaagcuaa aacggcagcc aagacccgaa cauuccguac uucuagaagg      180

cgguacgauc gcgucagaca aaggauugag uuuuugcagg acauauucgc gccuaucauu      240

gcuaaaaagg auuuugaguu cuuuaugcgg uuaaaggagu caaaauucca ccuggaagac      300

aagaaaaccg augggaaguu ugcccuauuc aaugacgcag auuuuacaga cguugaguau      360

uucgaucgcu acccaaccau cuaucaucuc cgagccgcuc ugauuaaacc gaaccacauc      420

acugacccca gacuucugua cuuggcaaua caucacauca uuaagaauag gggacauuuu      480

cucuucgccg gcacagauau ggagaguauc cggagcuucc agauugugua uacccagcug      540

uccgaacacc ugaagucuau caaccucgac uuugaguuac cuguagacgc ucaagauaaa      600

uucgccgcga uacugcuuaa ugaaaaguug aacaaacgug acaagaaaaa gcagcugaaa      660

gagcuaugcc cacacaagga uaagcgccuc gaguacgcau uuaaugccau uaucgguggg      720

aaaacgaagc ugaaagacau uuucggagug gaagaguacg augaaggcac uaagaaagcu      780

gucgacuuug agaaggccga uuaugacgaa gauaaggacg agaucgaguc ggugaucaaa      840

gaaaacauuc auguucuuaa ugcacugaag gcuguguacg auuggaccau auugacaaac      900

auccucggcg acuaugccua cauuucagag agccaggucg cgcuguauga agcccacaaa      960

aaggacuuaa gaauccugaa aagucucaua aagaaguacg caaaauccca guaugcugau     1020

gccuucaggc augagaaugu gaaggggaac uacgcuaacu acguaggauc uacuaauuau     1080

gacaacaaac aacccguuau ugauaauccu agcgugaccc aggaagacau caacaaguac     1140

uuugccgaua uccuucuguc cauuacacca gacgagucag aucgggagag uuaucaguac     1200

aucaccgaac gauugcacaa aggugaggca cuacccaagc ugcgcacgaa ggccaauagc     1260

guccucccuc accagauuca uaaaauagaa cuggagaaga uccuugcuaa cgcggcuaaa     1320

aauuucccau uucugcaaga aaaggacucu uccggcuaua cugccgcuca gaaaauugug     1380

aagaucauga uguucagaau cgaguacuau guggggccgu ugaacacaua ccaccauguc     1440

gaucagggag gccacgccug gaugguuagg aaagcagagg guaagauuac ccccuggaau     1500

uuugacgaaa agguggauaa agaggccuca gcugaaaagu ucauacggaa caugacuaau     1560

aaauguacau accucaucgg gaaggacgua auuccuaaac aaagccuguu auauagucgc     1620

uacaaggugc ugaacgagcu aaauaaccuc aaggucaaug gagugccaau cucuguugcc     1680

cugaaacaga agauuuauga ggaacuuuuu aaaaagcaag ugaacgucac cuucaaccag     1740

uugaaaacgu uccugaagac ccagggcgag gaugcagacg aaaucauacu cuccggcgug     1800

gacgaggcgg cucauaaguu uacaaauaac cugcagacuu accacaaauu cagagccauc     1860

cuuagggaua agauugaauu ugagccccag cguagcaugg uagaggacau cauucggugg     1920

aaaugccuga ugggggauga caucaccaug uggaagcaca aaauagaaaa ggaguaugga     1980

gaucaguugu cggaagaaca acucaagaaa auuacacgac ugaauuucaa cgguuggucu     2040

agauuauccc aggaauuucu gacugacguu ccuggcgugg auaucgaguc aggggagguc     2100

uacaguggaa uuauggacgc acuauggagg accaaugaaa acaucaugca gcuccugagc     2160

ucccgcuuca cauuugccgc ugaggugaag aaacagaauc aaggcaccgg ucggauagaa     2220

aagaucacuu augaugaggu caugaaagac acguaccuuu cuccagccgu gaagagaagc     2280

guuuggcagg caauuacauu gguggaagag aucaggaaaa uucgcaaggc ugaucccaag     2340

cgaaucuucg uagagaugac ccggcagccg gacgccaaaa aggaacguaa acugucaaga     2400

aggacucagc uccugaacgc guaccgcgcc auagggaaug auauugcuga guaugaaaag     2460

gagcauguca guaccuuaau cacacaacug gaguccaaaa aggacgaaga ccuucggaga     2520

aagaaauugu accucuauuu uacccagaug ggaaggugua uguacacugg cgagcgaauc     2580

gaucugagcu cucuacugcg cgacacaaac ucaaauauuu augauaucga ccacauuuac     2640

ccucggaguc ucacgaagga ugacagccug gauaacaccg ugcuuugcaa aaaggaaaua     2700

aauuugaaaa agggcaacaa guauauccau agaguuuuca cugacaaaga gcugaagaaa     2760

caguacgggu acuggaaggc auuaaaagaa aagaagcuca uuuccgagag gaaauuuucu     2820

cgucugaugc gcacaaccga ucuuacagcc gacgagcugg cuggauucau cucgcggcaa     2880

uugguggaaa cuagccagau ggucaaggcc accgcagaua uucucgagaa aaucuauccc     2940

aaugugacga uaguauacgu gaaggcgaga accguuuccg acuuuaggca cgacuaugaa     3000

cugccaaaag uccgagcccu aaacgauugu caccaugcuc acgacgccua ccugaacauc     3060

guggugggua augucuauua cgagaaguuc acaaagucac cuuuccgguu uauugcaaac     3120

cagaaaggca agggggauau caaauauaau cucuaccgcc uguucgacaa ggauguuaaa     3180

gugggagaca gaauuaucug gaacgcuaag gaagaucuug ccagggugaa agcaacuuug     3240

gagcgggaca ccguaaaugu cgugcgaaug cccguugagc agagaggcca auuuuucgau     3300

gccacaauac agaagaaggg uacuaccaaa aacaugcugg uguuaccacu caaggggagu     3360

gacgaacgcc ugcaggauac gucuaaauac ggcuauuacg ucaggcccag cggaaugucc     3420

uuuauggugg uucagcauga ggugaagggc aaaucacgug uaacaauuga accugcacuu     3480

cuggucuaua agaauaagcu aaaaagcgag gacgauuacg cgaaguauug cgaagacgug     3540

cucaaacugc aaaagccaaa aguguuguuc aagaacauca aguuuggguc uaaaauccug     3600

uucgacggau acccguuuuu acucaagggu cggaccgaga auagaauuau aauggccgau     3660

gcacaguccc uguuccuugg caaccaggag cugaaaaucu ugaagagggu cacuaaaauu     3720

agugacgaua ucgaccacaa gguugaaagc cgcauuaaga ucacagauga ggaauggacc     3780

acucucugga aaucuguggu agagcagaua gugaccggga aguuucgaaa uagacuggga     3840

acacuaaacg gcauccugca aggucggaaa auuaaacuuu ccaaggacga ggaagcuagg     3900

cucaaugagc uggauucaaa ggaccaggaa acguucuuga gucagauccu guuagagcgc     3960

uuccuccaau uuagcaccga agagaaaacu cgucugcuuc ugcaggucau uucguucucc     4020

gggaacacaa ccggcgugga uuugagacuc aucggaggcu cuagcgggac aggaacuaua     4080

aagaccaaua agaacauuac ggaccauuca guuauccuga ucaacgagag ucccacaggc     4140

cuauuuacca aucggacugu gcugaaccag ccuuga                               4176


<210>  3252
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3252
augaaaagca uccugggccu cgaccuuggg accacaucca uuggaugggc ccuggugaaa       60

gagucugaaa acgauaauga gaagucagaa auagucaaau uggguguuag auuagugccc      120

cugacuacgg acgagaggac cgauuucgaa aagggcaaaa guaucacaac uaacgcugac      180

cggaccagca agcgcuccau gcgacguaau cuacagagau acaagcucag gcgggaggca      240

cugguaucug agcuuaaaaa ggaacacugg auugaugagg gguaucgcuu uucggaagac      300

ggaccuggcu caacauucag cacccugaga uugagggcca aagcggcuga ggaggaaauc      360

agucuccaag aucuggccaa ggugcugcuc cagauuaaca aaaagcgggg uuacaagucc      420

ucucgcaaau uaaauagcgc agaggacggg caggccaucg auggaauguc cgucgcuaag      480

gugcuguaug accgaggccu uacuccaggc gaauacguuu aucagagaau guuggauggg      540

caauacgcaa uaccggaguu uuauaaauca gaccugcagg ccgaauuuga uaagaucugg      600

aaccuacaga aaaguuacua ccccgagcuc acagcugagc uguaugacaa acuuaccgac      660

aaaaauaaga gccagacgug ggccauuugc agggaugaac ugggagugca agguuuuaag      720

ucuuccuuca cuggcaaaga gcgguugaag gaagcguacu uuaaccguca gcaggcauca      780

aaacagaagg ucgaccucga ggugcugacc uguguauuac aagauaucaa ugggcagaaa      840

gccagccuga guggacuccu uucugcuauu uccgaccgca gcaaggaacu guauuucaac      900

agaaugacag uuggccagau guuggugagg caaauccagg aggauccuaa ucggucgcua      960

cgaaacacug ucuuuuaccg ccaggacuau auagaugagu ucgaaaagau uuggucugug     1020

caguccagau uucauccaga gcugacccaa gaaaggaaaa agaaacucaa ggacaucauu     1080

aucuucuacc agcggccccu gaaaucacag aagggucuua ucagucugug cgaguuugaa     1140

agccgcgagg uggauauuuc cgucgacggg guuauaaaga aaaagaaaau cggaccuaga     1200

guguguccaa ggucuagccc cuuguuccag gaguucaagg uauaucaaac agugaauaac     1260

auucucguca auggcgaacc ucuggaugac aacgaccgug agaaauuagu gggcgaacug     1320

gccuuucggg agaagaugaa ggggcaggau guucuaaaac uccuguacaa gaccucaaaa     1380

ggaaugcgaa ucaacuucga ggaaguggag gguaauagaa cgauggcaac uauacuuaag     1440

gcuugcgaaa agauugucga gggcuugggg uacgacacaa gggaucugag uaagaucacc     1500

gccuccgaaa aagugagccg caucgaggag auuuuugcga aggaaggauu caagucugac     1560

uuucuccacu ucgauucaag ucugccaagc ccggaguuug aaucccaggc cucuuauucg     1620

cuuuggcauc uguuguacag cuaugagaac gacaaauccg uaucaggcaa ugaaagucuc     1680

guugagaaga ucaaaaagcu guguucuaug gaugaggcaa gcgcuaaaau uuuauccggu     1740

ugcacuuucc ugucagacua cgggagccua ucugcccggg cuaugagaaa gauacucccc     1800

uauaugaagg auggccugga auacuccgcc gcaugugcca uggcuggaua uaggcacagu     1860

aaacgcagcc uuacaaagga ggaauuggac ucucgagugu acgucgaucg gcuggagcuc     1920

cugccuaaaa acuccuuacg uaauccagug gucgaaaaga uccugaacca gaugauuaau     1980

gugguuaacu cagugguaga caccuacggc aaacccgaug agaucagaau cgagauggcg     2040

agggaacuua agaagagugc aagcgagcgc gaaaaagccc aaaaggacau ugcugccgca     2100

ucgaaaguca augaguccau aguggccacg uugaaggggu cuccuuucuu caucgacagc     2160

ccaucacgga acgauauuau cagauauagg cucugggagg aacugaaacc caauggauac     2220

aagacccuau auagugguac auacauuccg aaggagcgac uguuuuccaa agacuucgau     2280

aucgaacaua uaaucccuca ggcucuccug uuugacgauu cuuucgccaa caagacuauu     2340

gagagccgcc agugcaauau cgaaaaaucc aaccggaccg caauggacua uguuaaguca     2400

aaauacgaua gcuucggcgu gcaggcguau auugacagag ucaaggggcu uacaggagug     2460

aguaaaacua agaucaccag guugcugaug gagucuaagg auauacccga ggacuuuauu     2520

aaucgugaau uacgcgauuc ccaauacauc gcucggaaag ccagagagau ucucgaaagc     2580

augguaaagu ucgugguucc aacaaccggc ucggucacug caaggcugcg agaggacugg     2640

cagcuuguga acgugaugcg ggaacugaac ugggauaaau acgagaaguu gggucucacg     2700

ucauuucgcg aggacagaga cgggagggaa aucccuguca uagaggauug gacaaaacgg     2760

aaugaccacc gacaccaugc cauggaugcu cugaccaucg ccuucacuag acgccaguuu     2820

auucaauauc uaaaccaccu gaaugcaaag aucgacaccg uugauaagac agacauugau     2880

cucagggacu acaaccugga agauaucucu uucgacggcc uucgugaugg agacaaauuu     2940

ggcauaguga aggcuuugca gaaaaaguau cuguacaaag acgauaagca gaaguaucgg     3000

uucgugcccc caaugcccuu agacgaguuu agaauugaag ccaaaaggca gaucgaggau     3060

guacucguca guuucaaggc gaaaaauaag guguguacca aaaacguuaa uacuacaaag     3120

gugcgcgggg gacaacagcg aaagauccag cugacgccua gagguccacu ucaugaggac     3180

accauuuacg gcaaacggcu gcaguauguc acuaaggaag agaaaguugg gggauccuuu     3240

gaugaagcca agaucgcuag cgugugcaaa aagucuuaca gggaggcacu aaugcgccgu     3300

cucauggagu guggcgguga cccgaagaaa gccuucacag ggaagaacag ccuggaaaag     3360

aaucccgugu ggguagauga guuucacucc ggcgaagucc cuuugagagu gcggaccgug     3420

ucaauggcua gugucuacac aguucgcaag agcgugggac aaucucugaa cauugagaaa     3480

guauuagaca agcacauaaa agaaauccuc agggcccgac uggaggagua uggcgggaau     3540

gcauccaagg cguucucaaa ccuugaugaa aauccaauuu ggcugaacaa ggagaaagga     3600

aucgccguga agagagucac uauugacagu ggcaaaaauc ccaucccuuu gcggagcaag     3660

cucgauaaag acggucagau aaucuacggg gaaggacaga acccacagga guccgauuuc     3720

gugguucccg gcgguaacca ucacgcugcc auuuauagga ccccgucugg caagcugcaa     3780

gacguggucg ugucguuuua cgaagcaguu agccgcaaga aucuagggcu ggaggugauc     3840

gacagaaacu ucaaaaagga ugagggaugg gaauuucuuu ucacgcucaa acagaaugag     3900

uauuuugucu ucccuaaccc agcuaccggc uuuucacccg aagacuacga ucugacagac     3960

ccugccaaua acgcaauuau cucccccaau uuguauaggg uacagacugu gaguguuggg     4020

gauuaccggu uccgucauca ccuggagacc acaguggaag acaacgagaa guuacgcgau     4080

auagccugga aacgaauugg accaucuggc cucgucggug cugugaaggu aagaaucgac     4140

aaucugggga ggauccuugc ggugggagag uacgauuga                            4179


<210>  3253
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3253
auggagaaca agaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucauaacag augacuauaa aguucccucu aagaaaauga agguguucgg uaauacugau      120

aaacacuuua ucaagaagaa ccucauuggc gcccuucugu ucgacgaagg ggcaacggcc      180

gaggauagaa gguugaaacg gaccgcgcgc cgacguuaca caagaaggaa gaaucgguua      240

cgcuaucugc aggaaaucuu uucagaggaa auuaguaaac uagacagcuc cuucuuucau      300

agacucgaug acucuuuccu gguaccuaag gauaaaaggg gaucgaagua cccaaucuuu      360

gcuacucuug aggaggaaaa ggaguaucac aaaaaguucc cgaccauaua ccaucugcgg      420

aaacacuugg ccgacucaaa ggaaaagaca gaucuccgcc ugaucuaucu ggcacucgcc      480

cauaugauua aguaccgagg ccacuuuuua uacgaggaga gcuucgacau caaaaacaau      540

gauauucaaa agaucuuuaa cgaguucaua aguauuuaug acaauaccuu ugaagguucc      600

ucucugagcg ggcagaacgc ucagguggaa gcaaucuuca cugacaagau uuccaaauca      660

gccaagagag agaggguccu uaaauuguuc agugaugaaa agagcacauc ucuguuuucc      720

gaguuccuaa aacucaucgu gggaaaucag gcugacuuua agaagcacuu cgaucuggag      780

gaaaaagcuc cccuucaauu uucaaaggac accuacgaug aggaccugga aaacuugcuc      840

ggccagaucg gcgauggguu cacggaccug uuuuuaguug cgaaaaagcu guaugaugca      900

auucuccuua gcggaauacu gacugugacc gacccuagua caaaagcccc auugucugcu      960

uccaugaucg agcgguacga aagccaucag aaggaucuag ccgcacugaa gcaguucauu     1020

aaaaauaacc uccccaagcg uuauaaugag gucuuuucgg accaaucuaa agacgguuac     1080

gcuggcuaua ucgaugggaa gacuacccag gaggccuucu acaaauacau aaagaaccug     1140

cuuuccaagu uugaaggagc ggacuauuuc cuggauaaaa uugagcgcga agacuuuuug     1200

agaaagcaga ggacauucga uaacggcuca aucccucacc agauccaucu ccaagagaug     1260

aaugccauuc ugcggcgaca gggugaacac uacccauucu uaaaagagaa ccgcgagaag     1320

aucgagaaaa uucugaccuu uagaauaccc uauuacgugg ggccucuagc aaggggaaau     1380

cgggacuucg cuuggcucac gcgcaacagu gaucaggcca ucagaccaug gaauuuugaa     1440

gaaauuguag acaaggcuag cuccgccgag gaguucauca acaaaaugac uaauuaugau     1500

cuguaccuuc cggaagagaa gguuuugccc aagcacucuc ugcucuauga aacauuugca     1560

guguacaacg agcugaccaa agucaaguuc aucgccgaag gccuuaggga cuaccaauuu     1620

cuggauagcg gccagaaaaa gcagauugug aaucaguugu ucaaagagaa gcguaaggug     1680

acugagaaag acauaaucca uuaucuccac aacgucgaug gguacgacgg aauugaacug     1740

aaggguaucg agaaacaauu uaaugcuuca uuaaguacau aucaugaccu gcuaaagauu     1800

aucaaagaua aggaguucau ggacgauccu aagaacgaag aaauacucga gaauaucguu     1860

cacacccuga cgauuuuuga ggaccgggaa augaucaaac agcgacuugc gcaguacgau     1920

uccauuuucg acgagaaggu gaucaaagca uugaccagaa ggcauuauac aggcuggggg     1980

aagcugagcg ccaaacucau aaacggaauu ugcgauaagc agacuggcga caccauccug     2040

gauuacuuaa uugacgaugg uaagaucaau cgcaacuuua ugcaacugau aaacgacgau     2100

gggcuuucuu ucaaagaaau cauucagaag gcucagguag ugggcaaaac agacgacguc     2160

aaacaggugg uucaagaguu gccaggauca cccgccauca aaaagggcau ucuccagagu     2220

aucaaaauag uggaugaacu ggucaaggug augggguaug caccugagag cauuguaauc     2280

gagauggccc gggaaaauca gacuaccgcu agaggaaaga aaaacuccca gcaaagguac     2340

aagcgcaucg aggacucucu gaaaaaucua gccccagguc ucgauucgaa cauucugaag     2400

gaaaauccca cagacaacau ccagcuucag aaugaucgau uguuccugua cuauuuacaa     2460

aacggcaaag acauguacac cgggaagccg cucgauauug accagcugag cuccuaugau     2520

auagaccaca ucauuccuca ggcauuuauc aaggaugacu caauugacaa ucggguucuu     2580

acgaguucua aagauaaccg uggaaagagc gacaaugugc ccucccugga ggucgugcag     2640

aaaagaaagg cguucuggca acaguugcuc gauucaaaac ugaucagcga gaggaaguuu     2700

aacaaucuaa cuaaggcuga acgcggcggu cuggacgagc gggauaaagu cggguucaua     2760

agaaggcagc ucguggaaac acgacagauc accaagcacg uugcccaaau ucuggacgca     2820

ucuuuuaaca cugaggugaa ugagaaaaac cagaagaucc gcaccguaaa aauuaucaca     2880

cuuaagucca acuuggucag uaauuuccgg aaggaauuug agcuguacaa agugagagaa     2940

auaaacgauu aucaucacgc ccaugacgcu uacuuaaaug ccguuguggc aaaggcuauu     3000

cucaaaaagu auccaaaacu ggagccugag uucgucuacg gcgauuacca gaaguaugac     3060

cuuaagaggu acaucagccg uuuuaaaccc ucuaaggaaa ucgaaaaagc caccgagaag     3120

uauuucuuuu acuccaaccu gcuaaauuuc uucaaagagg aagugcacua ugcggaugga     3180

auuauaguaa agcgcgagaa caucgaauac ucaaaagaca cuggcgagau ugccuggaau     3240

aaggaaaagg auuuugcuac aaucaaaaag gugcucaguu auccacaggu uaacauuguc     3300

aaaaagacgg agauccaaac ccacgggcug gaccggggaa aacccaaggg uuuguucaau     3360

agcaacccuu cgccaaagcc guccgaggac ucuaaagaaa aucuggugcc cauaaagcag     3420

ggcuuagauc cuagaaaaua cgggggauac gcaggcauca gcaacucaua ugccgugcuc     3480

gucaaggcua uuguugagaa aggugccaag aagcagcaga aaacuauccu ggaauuucaa     3540

gggauuagua uccuugacaa gauaaauuuc gagaacaaua aagagaacua ccuguugaag     3600

aaaagguaua uugaaauccu cuccacaauc acccugccaa aguacucucu auuugaguuc     3660

cccgauggca cacgacggcg ccuggcaagc auucuuucca cuaacaauaa gagaggagaa     3720

auccauaaag gcaacgagcu cgugcugccu gggaaguaua ccacguugcu guaccacgcg     3780

aaaaauauaa acaagaaauu agaaccagag caucucgagu auguggaaaa gcacaggaau     3840

gacuuugcca aacugcuuga guguguacug aacuucaaug auaaguacgu cggagcuuug     3900

aagaacggcg aacggauucg acaggccuuu accgacuggg agacagugga uaucgaaaaa     3960

cucugcuucu cauuuauugg ucccgagaau agcaagaacg cagggcuguu cgagcuaacc     4020

agucagggau cugcuuccga cuuugaguuc cugggcguua aaaucccgag auaccgcgau     4080

uaugccccua gcucgcuucu caaggcaacu cugauacauc aaucaauuac agguuuguac     4140

gaaacuagga ucgacuuauc uaaacugggc gaagauuga                            4179


<210>  3254
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3254
augaacaaua acaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucauaacag augacuauaa gguucccucu aaaaagauga aagugcucgg uaauacugau      120

aagcacuuca ucaaaaagaa ccuuauuggc gcucuguugu uugacgaggg ggcaacggcc      180

gaagauagaa gguucaagcg gaccgcgcgc cgacguuaca caagaaggaa aaaucgguua      240

cgcuaucugc aggagaucuu uucagaagag augaguaagg uagacagcuc cuucuuucau      300

agacuagaug acucuuuccu cgugccugaa gauaaaaggg gaucgaagua cccaauuuuu      360

gcuacucugg ccgaggagaa agaauaucac aagaaguucc cgaccaucua ccaucuucgg      420

aaacaccugg cagacucaaa ggagaaaaca gauuugcgcc ucauauaucu ggcccuggcu      480

cauaugauca aguaccgagg ccacuuucuc uacgaagaga gcuucgacau uaaaaacaau      540

gauauccaaa agauuuuuag ugaguucauc uccauauaug acaacaccuu ugaagguucu      600

agcuuauccg ggcagaaugc acaggucgag gccauuuuca cugacaaaau cucaaagagu      660

gcuaagagag aaaggauucu gaaacuuuuc gccuacgaaa agagcacaga uuuguuuucu      720

gaauuucuga aacuaaucgu gggaaaccag gcggacuuua agaaacacuu cgaucucgag      780

gagaaggcac cccugcaauu uuccaaggac accuaugaug aagaccuuga gaaucuguug      840

ggccagaucg gcgaugacuu cgccgaucuc uuucugguug cuaaaaaguu auacgacgcc      900

auucugcucu cagggauacu uacggugacu gauagcagua ccaaagcacc ucugucugcu      960

uccaugaucg aacgguauga gaaccaucag aaggacuugg ccgcgcuaaa acaguucauu     1020

caaaauaacc ugcaggaaaa guacgacgag gucuuuagcg aucagucgaa ggacggauau     1080

gcccguuaca ucaacgguaa aacaacucag gaggcauucu acaaguauau aaagaaucuc     1140

cugucuaagu uugaaggcuc cgauuacuuc cuugacaaaa uugagcgcga agauuuucug     1200

agaaagcaaa ggaccuucga caacggguca aucccacacc agauccauuu gcaggagaug     1260

aaugcuauua uccggcgaca aggagaacac uaucccuucc ucaaggagua caaagagaag     1320

auugaaacaa uacugaccuu ucgcaucccu uauuacgugg gcccauuagc cagagguaac     1380

aggaauuucg cuuggcugac gcggaacagu gaucaggcca uucgccccug gaauuuugag     1440

gaaaucguag accaggcaag cuccgccgag gaguucauca acaaaaugac uaauuaugau     1500

cuauaccucc cugaagagaa gguucugcca aaacacucuc uuuuguacga aacauuugcu     1560

guguauaacg agcugaccaa ggucaaauuc auuagcgaag ggcucagaga cuaccaguuu     1620

cuggauucag gacaaaagaa gcagauagug aaucagcuuu ucaaagagaa gaggaaagug     1680

acugagaagg acaucauuca guaucugcau aacgucgaug gcuacgacgg caucgaauug     1740

aaagggauug agaagcaauu uaaugcgagu cucuccacau aucacgaccu guuaaagauc     1800

auaaaagaua aggaguucau ggacgauccg aaaaacgagg aaauccugga gaauauuguu     1860

cauacccuaa cgaucuuuga ggaccgugaa augauuaagc agcggcucgc acaguacgcc     1920

agcaucuucg auaaaaaggu gauaaaggcu cugacccgaa gacacuauac aggauggggu     1980

aaacuuucug ccaaguugau uaacggcauc ugcgacaaaa agacugggaa aaccauucug     2040

gauuaccuca ucgacgaugg auacucaaac aggaauuuua ugcagcugau aaacgacgau     2100

ggcuuaaguu ucaaggacau cauucaaaag gcacagguag uggguaaaac aaaugauguc     2160

aaacaggugg uucaggagcu gcccgggagc ccugccauca aaaagggcau ucuucaaucc     2220

aucaaauugg uggacgaacu cgucaaggug augggacaug cuccagaguc uauaguaauu     2280

gaaaucgccc gcgagaacca gacuaccgca cggggcaaaa agaacucgca gcaaagauau     2340

aagaggaucg aggacgcgcu gaaaaaucua gcucccgggc uggauagcaa uauucucaag     2400

gaacacccua cagacaacau ccagcugcag aaugaucgcc uuuucuugua cuaucugcag     2460

aacggaaaag acauguacac cggugaggcc uuagauauua aucaacucuc cucauaugac     2520

auagaucaca ucguuccaca ggcauuuauu aaggacgaua gucuggacaa ccgagugcuu     2580

acuucuagca aagauaaucg gggcaagucc gacaacgucc ccucacugga agugguccag     2640

aagcguaaag ccuucuggca gcaauugcuc gacagcaagc ugaucucuga gcauaaauuu     2700

aauaaccuaa cgaaggcuga gagaggggga cuggaugaaa gggacaaagu gggcuucauu     2760

cgccggcagc ucguugagac aagacagauc accaagcacg uggcccagau acuggaugca     2820

agguuuaaca cugaaguaaa ugagaaggac aaaaagaacc gaaccgucaa aaucauuaca     2880

cuuaagucca auuuggugag uaacuuccgc aaagaauuua agcuguacaa gguucgggag     2940

aucaaugauu aucaucacgc ucacgacgcc uacuuaaacg cgguggucgc caaagcuauu     3000

cucaagaaau acccgaagcu ggagccuggu uucguguaug gggauuacca aaaauaugac     3060

aucaagagau acauaagcag gucuaaggau cccaaagaag uagagaaggc aaccgaaaaa     3120

uauuucuucu acuccaaucu ucugaacuuu uucaaggagg aagugcauua ugccgacggc     3180

acuauuguua aacgugagaa uaucgaguac ucaaaggaua caggagaaau cgcuuggaac     3240

aaagagaagg acuucgccac gauuaagaaa guccuaaguc ucccacaggu gaauauagug     3300

aagaaaaccg aaauccagac ucacggccug gaucgcggga agccucgggg auuguuuaac     3360

agcaaucccu cgccaaaacc cuccgaggac agaaaggaaa accugguccc uauuaagcaa     3420

gguuuagacc caaggaaaua cggcggguau gcaggaaucu cuaauagcua cgcgguucuc     3480

gugaaggcca uuaucgagaa aggcgcuaag aaacagcaga agacagugcu ggaguuccag     3540

gguauaucaa uccuugauaa gauuaacuuu gagaaaaaca aggaaaauua ucuguuggaa     3600

aaaggguaca ucaagauucu caguaccauc acacugccga aauauucccu auucgaguuu     3660

cccgacggca cucgacggcg ccuggccucu auacuuagca ccaacaauaa gagaggagag     3720

auucauaagg gcaacgaacu cguaaucccu gagaaauaca cgacccuguu guaucacgca     3780

aagaauauca acaaaacacu ggaaccagag cauuuagaau acgucgagaa gcacaggaau     3840

gauuucgcua aacuccugga guacgugcuu aacuuuaaug acaaguaugu uggggcccug     3900

aaaaacggag aacggauucg acaagcauuc aucgauuggg agacugugga cauagaaaag     3960

uuguguuuuu ccuucauugg ccccagaaau ucaaagaacg ccggucucuu ugagcugacc     4020

agccagggga gugcuucuga uuucgaauuu cuaggaguca aaaucccucg cuacagggac     4080

uauacaccau ccagccugcu uaaugcgacu cucauucacc agucgaucac cggccuguac     4140

gagacgcgua uagauuuguc aaaguuaggu gaggacuga                            4179


<210>  3255
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3255
augaagaacu accacaucgg ccuggacauu gggaccagcu ccgugggauu cgccgcuaga       60

gaugacgaga auaaccucau aagggucaaa gguaagaaug uuaucggcgc acggcuuuuu      120

gccgaaggga aaacagcgca ggagcgccga auggcucgua cuucuagaag gcgguauaac      180

cgcagaaagu ggaggcugcg guuguuaaau gauauuuucc augacgaaau cgugcacaaa      240

gauaaggacu uuuucaaccg ccugaagcaa ucaaguauua gccccaaaga ugaccgaaag      300

aaauacuuug gaucccuacu cuucccugag cugucugauu cggaauuuua ucauaauggc      360

aaccagacga ucuaccaccu uagacauagg cugaugaccu caaaugagaa ggccgacaua      420

cgggagaucu auuuggcacu ccgucacauu guaaaauacc gcgguaacuu ccuggaugac      480

acaccaguga gcaguuuuga agccuccgau cugaagcucg ggucuuucau cccgacuuua      540

aaugaccugu uugagaccau ugacgucaac uauguguuca auagcgaaaa cacaaaucag      600

aucgagucca uacuuuugaa caacgaucug agaaauucag acaagaguaa acagcuaguu      660

gaucuccugu acaggaacgu gagcgacguc gaugacaagu cucuugagaa acaauuuaau      720

aaguuccgga aagauauuuc caaggaaauc gcuaaaucau ucgugggaag caaguuuaac      780

guaaauaagc uguuggcaua cgagaccgac gauggcagua aaauuucucu cuccuucagc      840

gacgccucgu cugaugacaa gaugcuggaa uuagcugaca accuggauga cgagcgaauc      900

aauauccuca acgcccuuaa aguuauuuau ucccgcguga gacugaauca gauaaucccc      960

aacggcaugg gguugucaga aaguaugauu gcgagcuacg agcuacacaa gaaacagcug     1020

aaugaucuca agaagcugcu uaacguccug ccuuuucaug agaaauaugc agugaaggug     1080

gccuacgcug ccuauaucgg aaauauggac gcacagaaau ucacucaagc ugaauugucc     1140

acaauagagu cucucaagga aaaagauagc auuaagaagg ucaucgacug caaaaccggu     1200

cacaucacga acgagucacu gauuaguguu aucaaucaga uugugguaaa cccauacuua     1260

gaaaacuccg auuuugagcu gauagacauc gugaagaaaa uugaucagga caucaagaau     1320

ggcgaguauc uacucaaaca gaggaacaau caaaacggga auauccccca ucagcugcac     1380

cagauggaaa uggaucaaau uauagagaag cagagcaagu acuacccuuu ccuuggagaa     1440

uugaacccaa augagaaccg gcgccacauc gccaaauaua agauuucuga acuggucgcg     1500

uuuagagugc ccuacuaugu uggcccucuc aucacuccag aggagcaggc caaaaccuca     1560

ggugcaaguu ucgcuuggau gaagaggaaa gaagacgggg agauuacacc guggaauuuu     1620

gaucagaagg uggaccguga agccacugcu agccgguuca ucaaacgacu gaccgucaag     1680

gacacauacc uuuuuaacga ggaugugcug cccgacaauu ccuugcucua ucaacaguuc     1740

aagguacuga acgaguuaaa uguugugaaa gucaacaaua agagacuguc ucaggccgaa     1800

aaacagggag cauuuaacga ucuauucaag gugaccaaaa cggucacugc caagaggcuc     1860

aagaacuacc uggugucgaa uuucaaccau cuugacacaa uagagaucac cggcuugagc     1920

gauggcgaaa aauuuaauaa cgggcugacu uccuauguug acuuccaaaa gauuuuugga     1980

gauaaaauca auaaccucga gcugauggac gauuucgaau cauuaauuga guggaguaca     2040

guguuugagg accgccacau cuucgaagag aagguacgga augaaauaaa cuggauuucu     2100

gagagcgaac ugaaacagcu ugucgcuaag agauacuccg guuggggcag guugucaagc     2160

aagcuccugg uggggaucaa uaacgaugag ggacgcaaua uuaucgagca gcuauuugaa     2220

accucugaga cguucaugga agcgauaucc cgaccugagg uuaaaaagca gaucgacgaa     2280

auuaacagug auuuuaucaa aaauaccgac auugagagcg ugcuggcaga cgccuacaca     2340

ucuccacaaa acaagaaagc uauccgggag acuuauaaga uagucaagga uauucagaaa     2400

gccaugggcg guaaggcacc cgaauccauc ucaaucgagu ucacccguaa uccugaagac     2460

aaaggggagc ucacaagaag uaggcauagc gagauuucgc gcaaguacaa agaacugucc     2520

aaggagaucu cuaaagaacu uaacgaugag uugaaaagca agaaaaacuc aauggacauu     2580

gauaaguauu accuguauuu uaugcagaug ggcaaagacu uauacgaugg aaagaguaua     2640

gacaucgaug accucaauaa cuaucagauu gaucacaucg ugccacaauc cuucuacaaa     2700

gacgacucuu uugauaaucg gguacugacu uacuauacca acaaucagaa gaagggcaac     2760

aauacacccc uuaacgggcu gaauauuagc aacgaaacca gaaaauugug gucccaucuc     2820

cacgaccugg gacuaaucuc aaagaggaaa uaccugaauc ucacuaugca gggucuggau     2880

agccuuagua aguauacgaa acacggcuuc auacgacgcc aauuggugga gacaucucag     2940

guuaucaagc uggucgccaa cauuuuaucc gacgaguuca gcgcugauaa uaccaagauc     3000

auugaaguga aagccaagau gaacucgcag augcggcauu cauuuaaucu cuacaaaauc     3060

agagagcuga acgacuauca ccaugcacuu gaugcguacc ugacuaccuu cauaggggga     3120

uaucuauaca auagguaccc gaagcuccgu ggcauguuug uguaugguga cuucaaaaag     3180

uuugauguca acgacgcuaa caauguuaag acacugaacu ucuugcacga uauuaccaau     3240

ccuucugaca aauuugugaa ccagaucuac gaugaacaaa guggggagcu gaucuuagac     3300

cgcaagcacg ccauugaaac uauaaagaag aucuaucagu acaaauucau gcucauuaca     3360

cgggaggugu ccacgaagga cgauaagcug agcgaccaga ccguauauuc ugcaaaaaga     3420

aucaauaacg ucgccagccc cauuccagug aagaaagauu ccaauccuga gcuuuacggc     3480

uuuaggacug gaaacguuga ccaucacaug gugaucguca aguaucccaa aaaggauuca     3540

uucguguaca aaguuguggg cauaccaauc cgauuuaugc aggaacugcg gaguagcaag     3600

aaucaaucug aguacgaaaa cacauugaau cagauuguac aggcuaagcu caaccgcaaa     3660

aaguucgacu ucagagucgu gguggauaau gucauguaug ggcagcugau ccaagacgga     3720

ggucagaaau uuacccuagg cuccucaaca uacagguaua acgccaagca gcugguucuu     3780

gauauggaca gucuccggau ucugagcgau gagaaauacc gaaauuccuu gacugacgau     3840

gaaaagaacu cugaguucau caacauauau caacaggugc ugaauguagu gaacgagaag     3900

uuugacgucu ucgacgugag agggacccgc gauaaauuaa cgaagggacu cgacaaauuu     3960

cugaauauua ccucgaacga uaaggaaauc guucuucuga aaaucuugag cggccuccau     4020

uguaaugcag cuagggugaa gauuaacgag aucgguauaa auacaucauu cggggaacug     4080

cagacuaccc acggcauugu ccuauccgag aacaguuaca ucauuuauca gucucccagc     4140

ggacuguuug agcguaaggu gcggcuuaaa gaccucuga                            4179


<210>  3256
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3256
auggccaaca agaccuggua ccugggccuc gacaucggga gcgcuuccgu gggaugggca       60

gccacagaua cugguuauaa aauuauaaga aagaauaaaa agaggcuuug gggcguccgg      120

cuguucgagg aagcgaaaac ggcugaggac cgccgagggu accguucuuc aagaaggcgg      180

uuggcccgca gaaaguggag guuaaaccug cuagaggagc ucuuuaccag ugaaguuaag      240

aaaguggaug agaauuucuu ucugcggcuu aaggagagcc aguaucacua cgaagacaaa      300

acacauaagg uacccuaugc aaucuucaac gauaaagacu acacugauaa ggacuauuac      360

aaggauuacc cuaccauuua ucaucugcgc uccaaauuga ugacagagga aaauccagac      420

auccgaaagg uguuucucgc cauucaccac aucauaaaga acagaggaca uuuccugcug      480

caaggccagu cuuuuaagga ugguaaucuc aacaauuuaa ucaaagagcu gcuugaguug      540

gacauucugc acgucggguu cgaagugacc gaggaaguug uggauaagau cgcugacauu      600

ucgcuagaga aaaagacuuc aaaggacaaa cucaacgaua ucaaggaacu guacccgaaa      660

gagaagcagc uucuggaggu cuuuagguug auauucggag gcaaaacaag ccucgacaag      720

cuguuugcaa uugaugaaua uaaggaguua gacgccgcua ucaaaagugu guccuucaag      780

gagaaaauuu acgaagaagu acggcacgau uaugagcagg uucugucuaa uuacaucgag      840

cuccuugacc uggccaaguu gguguaugau agcaucauuc uauccgacau uaaaaaggaa      900

ggcaagaccc ugucagagag uaaagucgaa cucuuugaga agcaucguga ugaccugcuu      960

aaacugaaga acuuggugaa aaaugauagc aagcucucug aagacaagaa aguggagcug     1020

uacgcguuaa ucuucaagga ggacaaagau aaggggacga acuacgucaa uuauauucgc     1080

aaauccgaag agggaaaggg uugcaacuac gaagacuuca aaaaguuucu gguuaaggag     1140

cuagcaaaac ucgaggaauc aagcgugaag acugagauca uaaaagaucu ggaacuugag     1200

caauucuugc cccugcagag aaccaaggac aacaguguag ugccuuauca gauucacaaa     1260

gaggaacucg ucaagauccu ggauaaugcc gcuagguacc auucuuuucu uaacgagaag     1320

gacgaauccg gcuauaaugu gcgggagaaa guuauccagc uguuggaguu ccgaauacca     1380

uacuaugugg ggccccucaa cagcucgaag aaagccaagg aaggaggcuu ugcauggucu     1440

guccgcaaua cagguuacga aaacacuccu gugaccccau ggaauuacuc caaaguaauc     1500

gaugagucag cuagugccga gaaguucauu acaaaccuga ccaauaagug uacguauuua     1560

aaaggggaag acguucugcc caagagcucc cuacucuacu cugaguuugc gcugcuuaac     1620

gaauugaaug cccugaaaua ugauggaaac agaauaagcc ucgaggcaag gaauacuauc     1680

auugaaaagc uguucaaaca acagggcaag aaggugacaa aaaccucaau caagaacuua     1740

cugaaagcug agggcuacau cgacgggaag ggagagauua cugguauaga uaucacaguc     1800

aaaaaugacc uuaagaguua ucgggauuuu aaaaagauuu ugggcaacaa guucaacucc     1860

gaacacgugg agaauaucau ucucuggauc acccuguacg gggaaagccg caaacuaaua     1920

aaggccaaaa ucgagucugu cuauggagag guguacucaa aggacgaaau uacgaaaaug     1980

aguagacugg uuuacaagga cuggggcagg uuuagccgua agcuccugac cgagcuugug     2040

uccaaaaagu uguauaacga agagacaggu gaaugccuga auaucauugg ggcuaugcgg     2100

cagaacaaua ucuuauucau ggagcuccug gccgaucgau uugacuacuc uaaacagaua     2160

guagaguuca acaaggaacu ucaagaggau gucacugaaa uugcaccuga gauccuggac     2220

gauuuguaug ugucgccagc cguuaaaaga agcauuuggc agaccgugag gaucgucgag     2280

gaacucaaga agauaaucgg cugugcuccg gcgaaaauuu ucguggagac aacucgcucc     2340

aaucaggaaa agaaaaagcc caccgacuca cggaaaaagc aacuggagcu agcauacaag     2400

gccguuaaaa aggaugugaa agaacuggag aaggaaaucg gaacaauuaa cuuugacguu     2460

cucaaugaua gacugaguuc ugucgagccu agcaaacuua aggcuaagaa auuguaucug     2520

uacuauaccc aguuagggag gugcauguac uccgaggaac caaucaacau aucagaacuc     2580

uucgacaaua acaaguacga uauugaccac aucuaucccc agagcaaaau caaggaugac     2640

ucuuuuacua auacggugcu ggugaaacgc gaauccaacg ccgcaaagac agaccgauac     2700

ccucuuggca gugauauuca gaccccaagc aaucggcguu ucuggaaauu ucugaaggag     2760

aagggacuaa ucucugacga gaaauauaac agacucguca ggacugaaga guucuccgau     2820

gaggaacugu cagguuuuau ugcccgccaa uugguugaaa ccagucagag cgugaaggcu     2880

guggccucga uacugggcga guuaaacccc gagacaucca ucuguuacuc uaaagcagaa     2940

aauguaagcu cauuccggca gaacuuuggg aagauuaaaa agggagacag agauaguaag     3000

aauaacgacc agcucaucaa agucagggag auuaaugauu aucaucacgc gaaggacgcu     3060

uaccugaacg ugguuguggg caaugucuau gauaccaaau ucacucgaaa cguguuuaau     3120

uucaucaagg acaaaaagga uggucgcaag uacucccuua acaaaguauu uuacgaaaau     3180

gugucugaca gcaagacagu agccugggag auggacaaaa cgauacauca agucgaaaag     3240

gugaugaaca auaacaauau cauggugacc cggagaacuu ccgagcagaa agggggccug     3300

uucgaugcaa cagucuauaa ggccaagguu gcugccaaag caaaggacgg aguguacuau     3360

ccguugaaaa ccucaaacag cguagugaag gaugucgcua aauacggcgg guauacaagu     3420

auuggaaucg ccuacuauuc uauuuucgag uacacuaucg ugaacaagaa aggugaagag     3480

aagauaacca ggauuauccc uauccccauu uacauauccc agaauaucaa ggacagcacg     3540

gcgcucauug aauuuggcaa aucgcagauc uucugccgug ccuaugagaa gauuaaagau     3600

uuuaagcugc uauaccgcaa acuguguauc gggucaaaga uaaacaucaa uaacuaucaa     3660

uacuauauug gaggcaaguc ugacgaacgg uucuacuaug auagugcuau ccagguuacc     3720

cuugacaaaa aguccgagaa auaccucaag auucugagca aauaccagaa uuggaagaag     3780

gagaacaaag auggugaauu gugggagaau aucacaaagg aaaacaauau agagcuguau     3840

aacaaauuag uggagaagau gaacucugaa auuuuuacua aaaagaaaag caauaaauac     3900

aaugagcuca acuccgaaga gaucagaaau aacuucauca agauuucagu cgaggaacag     3960

gcaaaagugc ugcuugagau ccugaauuug cucaccaaca agaaaaguau auuugaccua     4020

aagagcauug auaucaaagc cgcuaggggg aagauuucuu ucaaccugac aucccugauc     4080

caauuuucaa uaauugaaca gaguaucacu ggcuucuaug agaaggaaau caccauuauc     4140

ggagacaaag gcaaugauau ggagaacaau aacagcuga                            4179


<210>  3257
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3257
augaacaaua acaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucauaacag augacuauaa gguucccucu aaaaagauga aagugcucgg uaauacugau      120

aagcacuuca ucaaaaagaa ccuuauuggc gcucuguugu uugacgaggg gacgaccgca      180

gaagauagaa gguuaaagcg gacagcccgc cgacguuaca cuagaaggaa aaaucggcug      240

cgcuaucuac aggagaucuu cucaccugaa auuaguaagg uagacagcuc cuuuuuccau      300

agacucgaug acucuuuucu ggugccagag gauaaaaggg gaucgaagua cccgaucuuc      360

gcgacccuug cugaagagaa agaguaucac aagaacuuuc ccacaauaua ccaucugcgg      420

aagcaauugg ccgacucaaa agaaaaggca gaucuccgcc ugaucuaucu ggcccucgcu      480

cacaugauua aauaccgagg ccauuucuua uacgaggaga gcuuugacau caagaauaac      540

gauauucaga aaaucuucaa ugaauuuaua aguauuuaug acaacaccuu cgaggguucc      600

ucucugagcg ggcagaaugc acaggucgaa gccaucuuua cugacaagau uuccaaauca      660

gcuaagagag agagggugcu uaaguuguuc ccugaugaaa aaucaacagg acuguucagc      720

gaguuucuaa agcucaucgu uggcaaccaa gccgaguuca aaaagcacuu ugaccuggaa      780

gagaaagcgc cacuucaguu cucuaaggau accuacgacg augaccugga aaauuugcuc      840

ggccagaucg gggauggauu ugcagagcug uucguggccg cuaagaaauu auaugacgcc      900

auucugcucu ccgguauacu uacggucacu gaucccucaa ccaaggcacc ucugagcgcu      960

aguaugaucg aacgguacga gaaccaccag aaagacuugg ccgcgcuaaa gcaauuuauu     1020

cagaacaauc ugcaggaaaa auaugaugag guguucucug accaguccaa ggacggcuac     1080

gccggguaua ucaacggaaa gacaacucaa gaggcauuuu acaaauacau aaagaaucuc     1140

cugagcaaau ucgaaggcuc ggauuauuuu cuugacaaga uugagcguga agauuuccug     1200

aaaaagcagc gcaccuuuga caacgguucu aucccacauc agauccacuu gcaagagaug     1260

aaugcuauua ucagaaggca gggggaacau uaccccuucc uccaggagaa caaggagaaa     1320

auuaagaaaa uacugacauu ccggaucccu uauuacguag gaccauuagc ccgaggcaau     1380

ggcgauuuug cuuggcugac ccgcaacucc gaccaggcca uuagaccgug gaauuucgaa     1440

gagaucguug aucaagcauc aagugccgaa gacuuuauca acaagaugac gaauuaugau     1500

cuauaccucc ccgaggagaa agugcugccu aagcacagcc uuuuguauga aacuuucgcu     1560

gucuacaacg agcugacaaa agugaaguuu auugcggaag ggcucaggga cuaccaguuc     1620

cuggauuccg gacagaagaa acagauagug aaucaacuuu uuaaggagaa acggaagguc     1680

accgaaaaag acaucacuca guaucugcac aacguugaug guuacgacgg cauugaguug     1740

aaggggaucg agaagcaguu caaugcaucu cucagcacau aucaugaccu guuaaaaauu     1800

aucaaggaua aagccuuuau ggacgaugcu gaaaacgagg ccacccugga aaacauaauc     1860

cacacgcuaa ccauuuucga ggaccgcgaa augaucaagc agagacucgc acaauacgau     1920

ucacuguuug acgagaaagu gauuaaggcc cuuaucaggc gucauuauac aggauggggc     1980

aaguugagug cuaaacugau aaaugguauu ugcgauaaga aaacugggaa gaccauccuc     2040

gacuaccuga uugaugacgg cuauuccaac cggaauuuca ugcaguuaau caacgaugac     2100

ggacugagcu uuaaagauau aauccagaag gcccagguag ugggccgaac aaaugacguc     2160

aagcaaauug ugcacgagcu uccagggucu cccgcaauca aaaagggaau uuugcaguca     2220

aucaaaauag uugacgaacu cgugaagauu augggucaua cuccugagag uaucgucauc     2280

gaaauggcga gagagaacca gaccacagcu aggggcaaaa agaauagcca acagcgcuac     2340

aaacggauug aagaugcccu gaagaaccua gcaccagggc uggacuccaa uauccucaag     2400

gaguacccca ccgauaacau ucagcugcag aaugacagac uuuucuugua uuaccugcaa     2460

aacggaaaag auauguauac uggcgagccg uuagacauaa aucagcucuc uucguacgau     2520

aucgaccaca uugugccuca ggccuucauc aaggaugaca gccuggauaa caggguacuu     2580

acguccucaa aagacaaucg cgguaagagu gacaacguuc ccucucugga agugguccag     2640

aaacgaaagg cuuuuuggca acaguugcuc gauagcaagc ugauuuccga gcggaaauuc     2700

aacaaucuaa caaaggccga acgugggggc cuggacgaga gagauaaagu gggauuuauc     2760

aggcgccagc ucgucgagac ccggcagaua acuaagcacg uggcacaaau ccuggacgcu     2820

agauucaaca ccgaaguuac agagaaagau aagaagaaua ggaacgugaa aauuaucacc     2880

cuuaagucaa auuugguaag caacuuucga aaagaguuca agcuguauaa aguccgcgaa     2940

auuaaugacu accaucacgc ccaugaugcg uauuuaaacg ccgugguugc uaaggcaauc     3000

cucaagaaau acccaaagcu ggaaccugag uuuguguacg gcgacuauca gaaauacgau     3060

cuuaagcggu auauaucuag auccaaagac cccaaggaug ucgagaaggc cacugaaaaa     3120

uacuucuuuu auaguaaucu gcuaaacuuc uuuaaggagg aagugcacua cgcugacggg     3180

acaauuguaa aaagggagaa uaucgaauau agcaaggaua cgggagagau cgccuggaac     3240

aaagagaagg acuucgcaac cauuaaaaag gugcucucuc ugccacaggu uaauauaguc     3300

aagaaaacug aaauccaaac acacgguuug gaccguggca agccccgcgg gcuguucaac     3360

uccaauccuu caccaaaacc gagugaggau agcaaggaaa acuuagugcc cauuaaacag     3420

ggacucgacc cucggaagua cggcgguuac gcggggaucu cgaacuccua ugccgugcug     3480

gucaaggcua uuaucgagaa aggcgccaag aaacagcaga agaccguucu ugaauuucaa     3540

ggaauaucua uccuggauaa aauuaauuuc gagaagaaca aggagaauua cuugcucgaa     3600

aaaggcuaua ucaagauucu gagcacaauc acucuaccaa aauacucacu guuugaguuc     3660

cccgacggga ccagaaggcg acuugcaagu auacucucca cgaacaauaa gcggggagaa     3720

auucauaaag gcaacgagcu ggugaucucu gagaaguaua ccacauugcu guaccacgcu     3780

aagaauauca acaaaacuuu agaaccugag caucucgaau auguggagaa gcaccgcaau     3840

gauuuugcca aacugcuuga aagcguacug gacuucaacg auaaguacgu cggugcauug     3900

aaaaaugggg agagaauuag gcaggccuuu aucgacuggg agaccgugga uauagaaaag     3960

cucuguuucu ccuuuauugg accacggaac aguaaaaaug cuggccuguu cgagcuaaca     4020

agccagggua gugcgucuga cuuugaguuc cugggcguua agaucccccg auacagagau     4080

uauacuccgu ccagccuucu caacgccacc cugauucauc agucgaucac gggguuguac     4140

gaaacacgca uagacuuauc aaagcuggga gaagauuga                            4179


<210>  3258
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3258
augaacaaua acaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucauaacag augacuauaa gguucccucu aaaaagauga aagugcucgg uaauacugau      120

aagcacuuca ucaaaaagaa ccuuauuggc gcucuguugu uugacgaggg ggcaacggcc      180

gaagauagaa gguucaagcg gaccgcgcgc cgacguuaca caagaaggaa aaaucgguua      240

cgcuaucugc aggagaucuu uucagaagag augaguaagg uagacagcuc cuucuuucau      300

agacuagaug acucuuuccu cgugccugaa gauaaaaggg gaucgaagua cccaauuuuu      360

gcuacucugg ccgaggagaa agaauaucac aagaaguucc cgaccaucua ccaucuucgg      420

aaacaccugg cagacucaaa ggagaaaaca gauuugcgcc ucauauaucu ggcccuggcu      480

cauaugauca aguaccgagg ccacuuucuc uacgaagaga gcuucgacau uaaaaacaau      540

gauauccaaa agauuuuuag ugaguucauc uccauauaug acaacaccuu ugaagguucu      600

agcuuauccg ggcagaaugc acaggucgag gccauuuuca cugacaaaau cucaaagagu      660

gcuaagagag aaaggauucu gaaacuuuuc gccuacgaaa agagcacaga uuuguuuucu      720

gaauuucuga aacuaaucgu gggaaaccag gcggacuuua agaaacacuu cgaucucgag      780

gagaaggcac cccugcaauu uuccaaggac accuaugaug aagaccuuga gaaucuguug      840

ggccagaucg gcgaugacuu cgccgaucuc uuucugguug cuaaaaaguu auacgacgcc      900

auucugcucu cagggauacu uacggugacu gauagcagua ccaaagcacc ucugucugcu      960

uccaugaucg aacgguauga gaaccaucag aaggacuugg ccgcgcuaaa acaguucauu     1020

caaaauaacc ugcaggaaaa guacgacgag gucuuuagcg aucagucgaa ggacggauau     1080

gcccguuaca ucaacgguaa aacaacucag gaggcauucu acaaguauau aaagaaucuc     1140

cugucuaagu uugaaggcuc cgauuacuuc cuugacaaaa uugagcgcga agauuuucug     1200

agaaagcaaa ggaccuucga caacggguca aucccacacc agauccauuu gcaggagaug     1260

aaugcuauua uccggcgaca aggagaacac uaucccuucc ucaaggagua caaagagaag     1320

auugaaacaa uacugaccuu ucgcaucccu uauuacgugg gcccauuagc cagagguaac     1380

aggaauuucg cuuggcugac gcggaacagu gaucaggcca uucgccccug gaauuuugag     1440

gaaaucguag accaggcaag cuccgccgag gaguucauca acaaaaugac uaauuaugau     1500

cuauaccucc cugaagagaa gguucugcca aaacacucuc uuuuguacga aacauuugcu     1560

guguauaacg agcugaccaa ggucaaauuc auuagcgaag ggcucagaga cuaccaguuu     1620

cuggauucag gacaaaagaa gcagauagug aaucagcuuu ucaaagagaa gaggaaagug     1680

acugagaagg acaucauuca guaucugcau aacgucgaug gcuacgacgg caucgaauug     1740

aaagggauug agaagcaauu uaaugcgagu cucuccacau aucacgaccu guuaaagauc     1800

auaaaagaua aggaauuuau ggacgauccg aaaaacgagg aaauccugga gaauauuguu     1860

cauacccuaa cgaucuucga ggaccgugaa augauuaagc agcggcucgc acaguacgcc     1920

agcaucuucg auaaaaaggu gauaaaggcu cugacccgaa gacacuauac aggauggggu     1980

aaacuuucug ccaaguugau uaacggcauc ugcgacaaaa agacugggaa aaccauucug     2040

gauuaccuca ucgacgaugg auacucaaac aggaauuuua ugcagcugau aaacgacgau     2100

ggcuuaaguu ucaaggacau cauucaaaag gcacagguag uggguaaaac aaaugauguc     2160

aaacaggugg uucaggagcu gcccgggagc ccugccauca aaaagggcau ucuucaaucc     2220

aucaaauugg uggacgaacu cgucaaggug augggacaug cuccagaguc uauaguaauu     2280

gaaaucgccc gcgagaacca gacuaccgca cggggcaaaa agaacucgca gcaaagauau     2340

aagaggaucg aggacgcgcu gaaaaaucua gcucccgggc uggauagcaa uauucucaag     2400

gaacacccua cagacaacau ccagcugcag aaugaucgcc uuuucuugua cuaucugcag     2460

aacggaaaag acauguacac cggugaggcc uuagauauua aucaacucuc cucauaugac     2520

auagaucaca ucguuccaca ggcauuuauu aaggacgaua gucuggacaa ccgagugcuu     2580

acuucuagca aagauaaucg gggcaagucc gacaacgucc ccucacugga agugguccag     2640

aagcguaaag ccuucuggca gcaauugcuc gacagcaagc ugaucucuga gcauaaauuu     2700

aauaaccuaa cgaaggcuga gagaggggga cuggaugaaa gggacaaagu gggcuucauu     2760

cgccggcagc ucguugagac aagacagauc accaagcacg uggcccagau acuggaugca     2820

agguuuaaca cugaaguaaa ugagaaggac aaaaagaacc gaaccgucaa aaucauuaca     2880

cuuaagucca auuuggugag uaacuuccgc aaagaauuua agcuguacaa gguucgggag     2940

aucaaugauu aucaucacgc ucacgacgcc uacuuaaacg cgguggucgc caaagcuauu     3000

cucaagaaau acccgaagcu ggagccugaa uuuguguaug gugauuacca aaaauaugac     3060

aucaagagau acauaagcag gucuaaggau cccaaagagg uagaaaaggc aaccgagaaa     3120

uauuucuucu acuccaaucu ucugaacuuc uucaaggaag aggugcauua ugccgacggg     3180

acuauuguua aacgugagaa uaucgaauac ucaaaggaua caggcgagau cgcuuggaac     3240

aaagaaaagg acuucgccac gauuaagaaa guccuaaguc ucccacaggu gaauauagug     3300

aagaaaaccg agauccagac ucacggacug gaucgcggca agccucgggg guuguuuaac     3360

agcaaucccu cgccaaaacc cuccgaagac agaaaggaga accugguccc uauuaagcaa     3420

ggauuagacc caaggaaaua cgguggcuau gcagggaucu cuaauagcua cgcgguucuc     3480

gugaaggcca uuaucgagaa aggagcuaag aaacagcaga agacagugcu ggaguuccag     3540

ggcauaucaa uccuugauaa gauuaacuuu gagaaaaaca aggaaaauua ucuguuggag     3600

aaagguuaca ucaagauucu caguaccauc acacugccga aauauucccu auucgaguuu     3660

cccgacggga cucgacggcg ccuggccucu auacuuagca ccaacaauaa gagaggcgaa     3720

auucauaagg gaaacgagcu cguaaucccu gaaaaauaca cgacccuguu guaucacgca     3780

aagaauauca acaaaacacu ggagccagaa cauuuagagu acgucgagaa gcacaggaau     3840

gauuucgcua aacuccugga auacgugcuu aacuuuaaug acaaguaugu uggcgcccug     3900

aaaaacgggg agcggauucg acaagcauuc aucgauuggg aaacugugga cauagagaag     3960

uuguguuuuu ccuucauugg acccagaaau ucaaagaacg ccggccucuu ugaacugacc     4020

agccagggua gugcuucuga uuucgaguuu cuagggguca aaaucccucg cuacagggac     4080

uauacaccau ccagccugcu uaaugcgacu cucauucacc agucgaucac cggacuguac     4140

gagacgcgua uagauuuguc aaaguuaggc gaagacuga                            4179


<210>  3259
<211>  4182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3259
augaagagag ugaccgaaga cuacauccug ggccucgaua uugggacaaa cagcugcgga       60

ugggccguca cugacaaaaa gaauaaccuu cugaaauuga gggguaagac ggcuauaggc      120

ucccacuuau ucgaagaggg gcauaccgca gccgaucggc gcggauuucg aacaacucgu      180

agaaggcuga aacggcgcaa guggagacua aggcuccugg aagagaucuu cgcggaaccc      240

auggcuaagg uugacccugg cuuuuucgug cggcuucacc agucuugggu aucaccacug      300

gauaaaaguc gcaagaaaua ucaugccauu guguuuccga ccgcaaagga ggacaaagcc      360

uucuaccaaa aguaucccac aaucuaccac uugcgagaug cucucaugac ccaggacaga      420

caguuugauc ugagggagau uuuccuggca auccaucaca uagucaaguu ucgggguaau      480

uuccuccagg acacuccugu gaaagauuuu gaagccagca agaucgaggu ugggccaauu      540

uuauccaaaa ucaacggagc uuucgccgaa aagauugugg aggaccaaga ucccaucgag      600

cugaaugucg cgaacgcagc cgaaauagag gcugugauuc guggcaaaga cgccgacaag      660

acacuuuaua aaauggauaa gguaaagaaa aucgcaaagu ugcugaccga cucuacggcu      720

aaagaagaga agaauguugc caaacagauu gcgaacgccg ugaugggcua caagacucag      780

uuugaaacca uccuagggaa ggagaucgau aaaucggaca aggcacagug ggaguucaaa      840

cucacagaug gagacgcuga ugacaagcug gaagcccuuc ugccugauuu ggacgagaau      900

gaucaaacua uuauagcuga aaucaaaaag cuauucucag ccauuacccu gagcacaauc      960

gucgacgaga acaagaguuu aucccagucu augguggcaa aauauaauaa gcaccaucag     1020

gacuacaaac ugcucaaggc cuacauagau acccuucagg accgcagcau ugcuaaaaag     1080

cuguugcuag cguaugaucu guacgugaac aauagacacg guaggcuccu ggaagcaaag     1140

acggaguuua aacaagacgg cacucuuaca aaggaggauu ucuauaaaac cgucaaggac     1200

aaccuggaug acuccgaauu ggcccggaaa aucgcucaga agaucgccgc agauaauuuu     1260

augccaaaac agcgaacuaa cucaaacggg guuauucccu uccaacucca ucagaucgag     1320

cuggacaaga uuauagccaa ucagaguaag uacuauccuu ucuuagcugc cgaaaaccca     1380

guggaggauc accgcaaaca ggcaccguac aagcuggacg aacucguaag auucagggug     1440

cccuauuacg ucggaccuau gaucacacca gaugagcaag agaaaaccag cggcaagucu     1500

uuugcgugga uggugcggaa agaagacggu cagauuacgc ccuggaauuu cgagcagaag     1560

guugaccgcc aggaauccgc uaacaaguuu aucaaaagaa ugaccaucaa ggauacauac     1620

cuccuggggg aggacgugcu uccugccaau ucauugcugu aucaaagguu cgagguccuc     1680

aacgaacuga auaacauucg uauaaaugga agccggauca guauugaucu uaaacagcag     1740

aucuuuaacg accuguucga ggaaaagaaa acugugaccg agaagucuuu gacauccuac     1800

cucaagcaga aucugcacuu accaacugua gaaauuaaag gccuggcaga ucccaccaag     1860

uuuaacagcu cgcuagccuc uuauuaccau cucaaauccc ugcacguuuu cgacaaggag     1920

cuugcugauc cgcaauauca gaaagacuuc gagaagauca uagaauacuc aaguaucuuu     1980

gaggauaaga aaauuuauca ggacaaguug caugccgaau uuaaauggcu gacaccugag     2040

caguuuaagg caaucagcac cuggcgacuc caaggcuggg ggagacuguc cuguagguua     2100

cugguggaac uucacgauac uaauggacag aacauuaugg agcaguugug ggacucucag     2160

aaaaauuuca ugcaaaucgu cacggagccc gauuuuaagg acgcuauagc caaagaaaac     2220

cagaacguga cacaggucaa ugguguggag gaaauucucg cggacgccua caccagccca     2280

gcuaacaaga aggcaauccg ccaaguugug aaaguagucg ccgauguggu uaaggcugcc     2340

ggcgggaaaa agccugcaca guucgcgauu gaguuuacuc gggaccccga uaaaaaucca     2400

cagcugucac auaucagagg aaccaagcua cugaaggccu accaggaaac agcuggcgag     2460

cucguggacc aaaaacugac cgauagucuu aaggaggcca ugacuuccag gaaauugcug     2520

aaggacaaau auuucuuaua cuuuaugcag acaggucgcg augcauauac ggggaagaag     2580

auaaacaucg acgaagucac cacuaauuac cagauugauc acauccuccc ccagagcuuc     2640

auuaaagacg auucuuuuga caaccgagug cugacagcua ccccucuuaa ugccgagaag     2700

ucagacgaug uaccauauaa acgguucgca aacaauuacg ugagugaccu gaagaugaca     2760

guuggcgaaa uguggaaaca cuggcaaaag gccggaauca uaaacaagca uaaauugggc     2820

aaucuccugc uagauccgga ccgucugaac aaguuucaga aaagcggguu cauuaauaga     2880

cagcucgucg agacuuccca aaucgugaag cuggugucug ucauccuuca gaacaaauau     2940

cccgaugcug agauuaucac cguuaaggcg ggagacaauu cggccuugag gcagcgccug     3000

aacuuauaca agagccggga ugugaacgac uaccaccaug caauugaugc uuaucucucc     3060

auaaucugcg guaauuuccu guaccaggug uauccuaaau acagaccaua uuuuguauac     3120

ggcaaguaua aaaaguucuc acaagacccc gaucuucaga aagaccugau uaagcacuuu     3180

aaaggguuca cguuuaugug gccucuacuc cagaaggaua acagugaaag gaaggcccca     3240

gaggaaauca aagagaauaa cucugaacga auugucuucu acaagcaccc cgacaucuuu     3300

gacaaacugc gcaaggccua caauuauaaa uacauguugg ugagccggga gaccacaacu     3360

gaguccucaa gcauguucaa gaugaccaua uuuccgagag gagaaaggga ucguaagaaa     3420

acacgcaagc ugaucccuaa aggcaacggu uuagacccaa agauuuaugg gggcuacucu     3480

ggaaauacug augcuuauau gguuaucgug aaaauugaca aggcaaagga guccaucuac     3540

aaagucauag gcgugcccau gcgggcccuc accagacuga accaggcgaa gaaacaaggg     3600

aauuaucagg aggaacuuca ucagguucug gaaccucaga uuauguugga uaagaacgga     3660

aaacccaaga ggggugugaa gggcuuccga guaaucaaag accacguccc auuuaagcaa     3720

guggugcucg auggggacaa aaaguucaug cuguacagug cucaguacaa aacgaaugcc     3780

aagcagcuag cacugagcca ggagacaaug cggaucguca ccgauaaccu uaaaaaggga     3840

gaggaccaag auaagcuccu gguuaaagcc uaugacgaaa uuuugcagaa gguggauugu     3900

uaccugucuu uauucgacac uuccaauuuu cgcaacggcc uccaucuggg uagaaauaaa     3960

uucauagacc uugagguaaa cgauaagaaa aucacacugu caaacauuuu gaaugggcuc     4020

cacgacaaca uggugcuggg caaucuaaag aacaucggaa uuggcacccc ucuggggcag     4080

augcaagaaa agaguggaau cacucuuagc cccgaggcug uccucauauu ucagucgcca     4140

accggccugu ucgagaaaag ggugaagauc gcagauuugu ga                        4182


<210>  3260
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3260
augaagaaag aaaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggu       60

ugggccguua cagacacuga uuauaaacuu cugaaggcua acagaaaaga cuuguggggc      120

augaggugcu uugaaacggc agagaccgcc gaagugcggc gcuuacaccg aggggcgcgu      180

agaaggauug agcggcgcaa gaagagaaua aaacugcuac aggaacucuu cucccaagag      240

aucgcuaaga uugaugaggg auuuuuccag aggaugaaag aaucucccuu uuacgccgag      300

gacaagacaa uccugcagga aaaugcacuu uucaacgaua aagacuuuac ugauaagacc      360

uaucauaagg ccuacccuac aauuaaucac cugaucaagg cuuggauaga gaacaaggua      420

aaaccagacc cgcgguugcu cuaucuggca ugucauaaua ucauuaagaa acgcggccac      480

uuccuguuug agggugauuu cgacucagaa aaccaguuug auaccaguau ccaagcccuc      540

uucgaguacu uacgagaaga cauggaggug gacauugaug cugacagcca gaaggucaaa      600

gaaauccuga aggauuccuc ucuuaaaaau ucggagaaac agucaagauu gaacaagaua      660

cuggggcuaa aacccagcga caagcagaaa aaggccauua cuaaucucau caguggaaac      720

aagauuaauu uugcggaucu guaugacaac ccugaucuua aagacgcaga gaaaaauucc      780

aucucuuuca gcaaagauga cuucgaugcc cuguccgacg acuuggcuuc aauccucggc      840

gauaguuuug aacuguuacu gaaggccaaa gcaguguaca acugcagcgu ucucucuaag      900

gugauuggcg acgagcaaua ccuuuccuuc gcuaagguca aaauauauga aaagcauaaa      960

acagaucuga ccaaguugaa agccgugauc aagaagcacu uuccaaauaa cuacaaaaag     1020

guauucgggu auaaccagga caaugaguca aagaacaauu acagcggaua uguuggugug     1080

uguaagacga aaaacaagaa acuggucauu aauaacagug ugaaucagga agauuuuuac     1140

aaguuccuga agcccauccu cucugcgaaa uccgagauaa aggaggugaa cgacauucug     1200

gccgaaaucg agacuggcaa uuuucuuccu aaacagauca gcaagucgaa cgcagaaauu     1260

ccauaucaac ugaggaaaau ggaguuggaa aagauccucu cuaaugcuga gaagcacuuc     1320

uccuuucuga aacagaagga ugagaaaggg uuaucacaua gugaaaagau uauaaugcug     1380

cuaaccuuca aaauccccua cuacauugga ccuaucaacg acaaucacaa aaagagcuuu     1440

ccagaucggu gcugggucgu uaaaaaggag aaauccccgu cuggcaagac aacucccugg     1500

aacuucuuug accauaucga uaaagaaaag accgccgagg aguucauuac aagccguacc     1560

aauuucugua cguaucucau aggugaauca gugcugccua agaacagucu uuuguacucc     1620

gaguauacug uacugaacga aaucaauaac cuccagauua ucauugacgg gaaaaauauc     1680

ugcgauacaa agcugaaaca aaagauauac gaggaccuuu uuaaaaagua uaaaaagauc     1740

acccagaagc agauuagcac uuucaucaaa cacgaaggaa uuuguaacaa gacagacgag     1800

gugaucauac ugggcauuga uaaagagugc accucuucau ugaagaguua caucgaacuc     1860

aaaaauauuu uuggcaagca ggucgacgag aucagcacga agaacaugcu ggaagagaua     1920

auccgcuggg cuaccauuua ugaugaaggg gagggaaaaa caaucuuaaa gacuaaaauu     1980

aaggccgagu acgguaaaua cuguuccgac gaacaaauca agaagauacu gaaucuaaaa     2040

uucucuggcu gggggagacu cucgaggaag uuucuggaga ccgugacaag cgaaaugcca     2100

ggauucuccg agcccguuaa cauuaucacu gcaaugcggg aaacccagaa uaaccuuaug     2160

gaguugcugu caagugaguu uaaauucaca gaaaauauca agaaaauuaa cucuggcuuu     2220

gaggaugccg aaaagcaguu cagcuaugac ggucucguga agccucuguu uuuaucccca     2280

ucagucaaaa agaugcugug gcagacccuu aaauugguga aggagaucag ccacauuacu     2340

caagcucccc cgaaaaagau auucaucgag auggcgaagg gggcagaacu cgagccugcc     2400

cgaacgaaaa cacgccugaa gauucuacag gaucuguacu cuuccugcaa aaaugacgcu     2460

gaugccuuua guagcgaaau ccaguaucuc ucuggcaaga uugagaacga agacaaucug     2520

agacuuaggu ccgauaaauu guaccuguau uacacccagu uaggaaagug uauguauugc     2580

ggcaaaccca ucgagauagg gcauguauuc gacacuucaa acuacgauau cgaccacauu     2640

uacccacaaa guaagaucaa ggacgauagc auuucgaacc ggguucucgu gugcuccucu     2700

uguaauaaaa acaaggagga caaauauccu cuaaagagcg aaauccaguc aaaacagcgc     2760

ggauucugga auuuucuuca gagaaacaau uucauaaguc uggagaaguu gaacaggcuc     2820

acccguacaa cccccauuuc cgaugacgaa acugcaaagu uuaucgcccg gcaacugguc     2880

gagacacgac aggcuacgaa aguggccgca aagguccuag aaaagauguu cccagagacc     2940

aagaucgugu acucuaaagc ggagacuguu agcauguuua gaaauaaguu cgauauuaua     3000

aagugcaggg aaaucaacga cuuucaucac gcucaugaug ccuaucugaa uauuguggua     3060

gguaacgucu acaauacaaa auucaccaac aaucccugga acuuuaucaa ggagaaacgc     3120

gacaauccua agauugcaga uacauauaac uacuauaaag uguucgacua cgauguuaag     3180

cggaacaaua ucacugccug ggaaaagggc aaaaccauaa ucacggugaa ggacaugcuc     3240

aaaagaaaca ccccaauuua cacaaggcag gcugccugua agaaaggggg acuguuugac     3300

caaacuauca ugaagaaagg ccuuggucag cacccguuga agaaggaggg gcccuucucc     3360

aauauuucaa aauauggcgg auacaacaag gucagcgcag cuuauuacac ccugaucgaa     3420

uaugaggaga aaggcaauaa gauacgcagu uuagaaacaa uuccucucua ccuggugaaa     3480

gauauccaga aggaccagga uguacuuaag ucuuaucuga cugaccuacu cgggaaaaag     3540

gaguucaaaa uccuggugcc aaagauuaaa aucaacuccu ugcugaagau aaauggauuu     3600

cccugccaca uuaccgguaa gacgaacgau agcuucuuac uccgaccugc cguucaauuu     3660

uguugcucga augacgaagu ccuguacuuc aaaaagauca uucgguuuuc agagauccgu     3720

ucucagagag agaaaauagg caagacaauu aguccauacg aagaucuuuc cuucaggagc     3780

uauaucaaag agaaccugug uaagaagacc aaaaaugacg aaaucgggga gaaggaauuu     3840

uacgauuugc uccagaaaaa gaaccuggag auuuaugaca ugcuacugac uaaacauaag     3900

gauacaaucu acaaaaagcg ccccaauucu gcgaccauug acauacuugu gaagggaaaa     3960

gagaaguuca aaagccucau cauugaaaac caguuugagg ugauccugga aaucuugaag     4020

cuguucuccg ccacucggaa ugucucagag uuacaacaca uuggcgguag uaaauauagc     4080

gggguugcua agauaggcaa caagaucucu ucccucgaua auugcauucu gaucuaccag     4140

ucaauuaccg gaaucuuuga gaaaagaaua gaccuucuga agguguga                  4188


<210>  3261
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3261
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggu       60

ugggccguua cagacacuga uuauaaacuu cugaaggcua acagaaaaga cuuguggggc      120

augaggugcu uugaaacggc agagaccgcc gaagugcggc gcuuacaccg aggggcgcgu      180

agaaggauug agcggcgcaa gaagagaaua aaacugcuac aggaacucuu cucccaagag      240

aucgcuaaga uugaugaggg auuuuuccag aggaugaaag aaucucccuu uuacgccgag      300

gacaagacaa uccugcagga aaauacucuu uucaacgaua aagacuuuac cgauaagaca      360

uaucauaagg cauacccuac cauuaaucac cugaucaaag ccuggauaga gaacaaggua      420

aaaccagacc cgcgguugcu cuaucuggcu ugucauaaua ucauuaagaa acgcggccac      480

uuccuguuug agggugauuu cgacucagaa aaccaguuug auacuucgau ccaagcacuc      540

uucgaguacu uacgagaaga cauggaggug gacauugaug ccgacagcca gaaggucaaa      600

gaaauccuga aggauuccuc ucuuaagaau agugagaaac agucaagauu gaacaagaua      660

cuggggcuaa aacccagcga caagcagaaa aaggcuauua caaaucucau caguggaaac      720

aagauuaauu uugccgaucu guaugacaac ccugaucuua aagacgcgga gaaaaauucc      780

aucucuuuca gcaaagauga cuucgaugca cuguccgacg acuuggccuc aauccucggc      840

gauaguuuug aacuguuacu gaaggcuaaa gccguguaca acugcagcgu ucucucuaag      900

gugauuggcg acgagcaaua ccuuuccuuc gcaaagguca aaauauauga aaagcauaaa      960

accgaucuga cgaaguugaa aaaugugauc aagaagcacu uuccaaaaga cuacaagaaa     1020

guauucgggu auaacaagaa cgagaaaaau aacaauaacu acucaggaua uguuggugug     1080

uguaagacua aaagcaagaa gcuaauuauc aauaacagug ucaaucagga agauuuuuac     1140

aaauuccuga agaccauacu cucugcuaaa uccgagauua aggaggugaa cgacauccug     1200

acagaaaucg agacuggcac cuuucuuccc aaacagauua gcaagucgaa ugccgaaauc     1260

ccuuaucagc ugaggaagau ggaguuggaa aagauucucu cuaacgcgga gaagcacuuc     1320

uccuuucuga aacaaaagga ugagaaaggg uuaucacaua gugaaaagau aaucaugcug     1380

cuaacauuca agauuccaua cuacaucgga cccaucaaug acaaccacaa aaagagcuuu     1440

ccugaucggu gcugggugau uaaaaaggag aaauccccau cuggcaagac cacgccgugg     1500

aauuucuuug accauauaga uaaggacaaa acugccgaag cauucaucac aagccguacc     1560

aacuucugua cuuaucucgu cggugaguca guucugccca agaguucccu uuuguacagc     1620

gaguauacag ugcugaauga aauuaacaac cuccagauca uuaucgaugg gaaaaauaua     1680

ugcgacauca agcugaaaca gaagauuuac gaggaacuuu uuaaaaagua uaagaaaauc     1740

acccaaaagc agauuucuac guucaucaaa cacgagggaa uauguaacaa gaccgacgaa     1800

guaauuaucc ugggcauuga uaaagagugc acaucaaguc ucaagagcua caucgaguug     1860

aagaauauau uuggcaaaca gguggacgaa aucuccacua agaacaugcu ggaggaaauu     1920

auccgcuggg cuaccauuua ugaugagggg gaaggaaaaa caaucuuaaa gacuaaaaua     1980

aaggccgagu acgguaagua cuguucugac gagcagauua aaaagauccu gaaucuaaaa     2040

uucucgggcu gggggagacu cagcaggaag uuucuggaaa ccgucacauc cgagaugccu     2100

ggauucucag aaccagugaa caucauuacc gcuaugcggg agacucaaaa uaaccuuaug     2160

gaauugcuga guucugaguu uaaauucacg gagaauauca agaagauuaa cagcggcuuu     2220

gaagaugccg agaaacaguu cuccuauaau ggucucguua agccccuguu uuuaucaccu     2280

agcgugaaaa agaugcugug gcagacacuu aaauugguca aggaaauauc ucacaucacc     2340

caggcaccac ccaagaaaau uuucaucgag auggccaagg gggcugagcu cgaaccggcg     2400

cgaacuaaaa cccgccugaa gauucuacaa gaccuguaca acaauugcaa aaacgaugca     2460

gacgccuuuu ccagugagau caaggaucuc agcggcaaaa uagaaaauga ggacaaccug     2520

agacuuaggu cugauaaguu guaucuguac uauacacagu uaggaaagug uauguacugc     2580

ggcaaaccua ucgaaauugg gcauguguuc gacaccucca acuaugauau cgaccacauu     2640

uacccccagu caaagaucaa agacgauagu auaagcaauc ggguacucgu uugcucgucc     2700

uguaacaaga auaaagagga caaguaccca cugaagucug agauucagag caaacaacgc     2760

ggauucugga acuuucuuca gagaaauaac uucaucucac uggaaaaguu gaauaggcuc     2820

acucguacaa cgccuaucag ugaugacgag accgcuaaau uuauugcccg gcagcuggug     2880

gaaacucgac aggcaacaaa ggucgccgcu aaagugcuag agaagauguu ccccgaaacc     2940

aagauagucu auuccaaagc cgagacagug ucuauguuua gaaacaaguu cgauaucauu     3000

aaaugcaggg agaucaauga cuuucaucac gcacaugaug cguaccugaa cauuguugug     3060

gguaauguau auaacacuaa guucaccaau aacccaugga auuuuaucaa agaaaagcgc     3120

gacaacccca agauagcuga uacguacaac uauuacaaag ucuucgacua ugaugugaag     3180

cggaauaaca ucaccgccug ggagaaaggc aagacaauua ucacuguuaa agacaugcuc     3240

aagagaaaua ccccuauuua cacaaggcaa gcagccugua aaaagggagg gcuguuugac     3300

cagacuauca ugaagaaagg ccuuggucag cacccauuga agaaagaagg ggcuuucagc     3360

aacauaucca aguacggcgg auauaauaaa gugucagccg cauacuauac ccugauugag     3420

uacgaagaga agggcaacaa gauccgcagc uuagagacga ucccgcucua ucuggucaaa     3480

gauauucaaa aggaccagga ugugcuuaaa aguuaccuga cagaccuacu cgggaagaaa     3540

gaaaucaaga uacugguacc caagauuaaa aucaauucuu ugcugaagau uaacggauuc     3600

ccuugccaca ucaccgguaa aacuggcgau uccuuugugu uacgaccagc uguucaguuc     3660

uguugcagca augacgaggu ccucuauuuu aagaaaauaa uucgguucaa cgaaauccgu     3720

ucgcagagag agaagauggg gaagacaauc ucacccuacg aggaucuguc uuuuaggagu     3780

uacauuaaag aaaaucuuug uaagaaaacc aaggccgacg agaucggaga aaaagaguuc     3840

uaugaucugu ugcaaaagaa aaaccucgaa auuuacgaca ugcugcuaac uaagcauaag     3900

gauaccauau auaauaaacg cccuaacucc acaacgcugg gcaugcuuac caagggucgg     3960

uacgaguuua ucaaucucaa accaaaggac cagauuaucg ugaugcugga gaucuugaaa     4020

cuguucugca cuacaagaga agcgauugau uuagggcuca uaaagggcaa gcccgccgcu     4080

ggaguggcaa cccugggcaa aaagaucagc aacccggaca auuguauucu uaucuaucag     4140

ucuauuacag ggaucuuuga gaaaaggaua gaccuguuga aggucuga                  4188


<210>  3262
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3262
augggggaca ccgugaagaa cuaccacauc gggcuggaua uuggaacaag cuccgucggu       60

uucgccgcua gagacgauga caauaaccuc auaaggguua aaggcaagaa ugugaucggg      120

acucggcuau uuucugaggg acagacggca caagaacgcc gaauggcccg ugcgucaaga      180

aggcgguaua accgcagaaa auggaggcug cgguuguuag augagauuuu ccaugacgaa      240

aucauucaca aggaugcuga guuuuucaau cgccugaaac agaguagccu aucccccaag      300

gacgaacgaa agcaguacuu uggcucucuc cuguucccug agguagguga uucggaguau      360

uaccaugacg ggaaccagac caucuaucac cuuagacaua ggcugaugac acaagaugaa      420

aaagccgaca uacgggagau cuacuuggca cuccgucaca uuaucaagua ucgcggaaau      480

uuucuggaug acacuccagu gagcucauuc gaagccagug accugaaacu cgcuuccuuu      540

gucccgaccu uaaacgaucu guucgagaca auugacguga auuacgaguu uucuauggau      600

aacaucaaug aaauagagga cauucuuuug aacaauaagc ugagaaacag cgauaaaucc      660

aagcagcuac ccgaccuccu gaccgaaccu aucguuaaua uugaugacaa gucacuugag      720

aaacaguuca acaaggaaag gacugauauc aguaaagaga ucgcaaagag cguggucggc      780

ucuaaaauga acgugaauaa gcuguugucc uuugagucaa gcggcgggua caaaauugcc      840

uucaguuucu cugacgcuuc cagcgaagau aagaugaugg agcucucgga cguacuggac      900

gauucucgga ugaacauauu aaauauccug aagguuauuu auucccgagu gcgccucaac      960

cagaucauac caucaggaau gggucuuagu gaaagcaugg ucgccgcgua cgaccugcac     1020

aaacaacagu ugaaggaucu uaaaaagcug cucaaugugc ugcccuuuaa cgagagauau     1080

gcagugaaaa uugccuacgc ugccuauauc ggcaauaucg acaagcagaa guucacaaaa     1140

uccgaaguca agaccauuga uaaacuuaag gagaaaucug caaucaugaa cguucuggac     1200

uuuaagagcg ggcauauuac gaaugaggaa uugucaaacg ugauaaaggu agugaucguc     1260

gauaaauacc ucaaugacga gaacuucgaa uaucacagua uuguggagaa gaucaaucag     1320

gauaucucca acggaagcua ccuguuaaaa caaaggaaua accagaacgg caauauuccu     1380

caucagcugc accaaaugga acuagaccag auaaucgaga accagucuaa guacuaucca     1440

uuucucggug agcugaaucc caacucaaau cggaaccaca uugcuaaaua caagaucagu     1500

gaacuuguug ccuuccgcgu gccuuauuac gucgggccau ugauuacucc ggaggaacag     1560

gagaagacca gcggagcguc cuuugccugg augaaaagaa aggaggaugg cgcaaucaca     1620

cccuggaauu ucgaagagaa aguggacaag gaagagacug cuucuagguu uauaaaacgu     1680

cugaccguaa aggauacaua ucuccugaac gaagacguuc uuccugacaa uucgcuguug     1740

uacgagaaau ucaaggugcu caacgagcug aaugucguga aggccaacaa uaaacgguua     1800

accgucgaag agaagcaagg cgcuuuugau aaccuguuca aaaagaugaa aacggugacu     1860

gccaagaagu ugaaaaauua ucucguuagc aaccauaacc acaucaauuc cguggaaauu     1920

acagggcugu cagacguaga uaaguuuaac aauagucuuu cuaccuacau cgacuucaaa     1980

aagauuuucg augacaaaau cgauaaccca gacuugcugg augacuuuga ggauauaauu     2040

gaauggagca cugucuucga ggaccgaaag aucuuugagg aaaaggugag aaaagauauu     2100

uccuggaucu cagagagcga aauaaaggca guugugucua aaagguacuc cggauggggu     2160

cgccucagua agaaacuguu aaauggcauc acaaacaaug aggggcggaa cauuaucgac     2220

gaacuguggg aggagagcga aaccuucaug gaggccgucu cuaagcccga agugaaggag     2280

cagauugacg gaaucaauuc cgauuuuaua aagaacacgg acauugaguc aguacuugcu     2340

gaugcguaua ccaguccuca gaauaagaaa gcaaucagag aaacacauaa guuggugaaa     2400

gacauccaga aggccauggg cggucaagcu ccagccagca uuucgaucga guucacuagg     2460

aacccccagg auaaagggca gcucacccgc ucccgacacu cugaaguuag ccggaaguac     2520

aaggagauuu caaaagaagu caguaagaca cugaaugacg agcuaaaauc caagaaaaac     2580

ucuauggaug uggacaagua uuaccuguau uuuaugcaga ugggcauaga ucucuacgac     2640

ggaagcucca ucgauauuga cgaccugaau aacuaucaaa ucgaucauau uaucccgcag     2700

ucauucuaca aggacgauag cuuugacaac cgugugcuua cucacuacag uaauaaccag     2760

aaaaagggca auaacacccc uuugaauggg cugaacauag augacaaaac aagagcaauc     2820

uggaaucacu uaagggaucu cggacugauu accaagaaaa aguauaacgg ucuuacucug     2880

aagggcaugg accaguuguc uaaauacacg aagcaugggu ucauccgccg gcaacucguc     2940

gagacauccc agguuauuaa acugguggcc aauauccuaa gcgaugaauu uucgaccgac     3000

gauacuaaga uaauugaggu gaaagcuaag augaacucac agaucagaca aguauucaau     3060

cuguauaagg ucagggaacu caacgacuac caccaugccg uggaugcaua ucugaccaca     3120

uuuaucggag gcuaccuuua uaaucgauac cccaacuugc gcaauauguu cguuuacggu     3180

gacuucaaaa aguuuucugc ggacguggau aaccugaaaa ccuucaacuu uuuacacgac     3240

auuacuaauc caagugauaa guucgaggac cggauauuug augaaaaauc cggggagcuc     3300

auccuggaca gaaagcacgc uauugagaca aucaagaaaa uuuaucagua caaguauaug     3360

cuuaucacga gggaagucag caccaaaaac ggccagcugu cugaucagac uguguacagc     3420

gccaagcgua uaaauaacgu uaaagcaccu auccccguga agaaagacuc caauccagag     3480

cuauauggau uccgcacagg caacguagau caucacaugg ucauugugaa guaccccaag     3540

aaagacucau augauuacaa gguggucggg aucccucucc gguuuguuca aaaacugaag     3600

gaaaccaaag acaauaagga uuacgagaac uugcugaauc agauugugca gaugaaguua     3660

aacaggaaaa agcaggacuu caucguagug gucgacaaug ugauguaugg acaacucaua     3720

caggauggug gcgaaaaaua cacacugggg aguagcacuu auaaguacaa cgccaaacag     3780

cuuguuauua auauggacaa gaccagaauc cuggaggaua accgauaucu aaauucucuc     3840

gacgagggag aaguguccga gaagcugguc aacaucuaca augauauuau gaaagugguu     3900

aacucacggu uugaccuauu cgauaucaac aaguuucgcg aaaaacugaa ugacggccuu     3960

aacaaguuca aaaauauaag uaacgagcag aaggaggaaa cgcuccugaa gauuuugagc     4020

ggucugcauu gcggguccgc uaaugugcca aucaaagagu uaggccucac cacaccgcug     4080

ggaaaacuuc aacugcccuc uggcauuguc uugucggaag aggccguagu gaucuaucag     4140

agcccuucag gccucuucga aaacaaaguu agacugaagg aucuauga                  4188


<210>  3263
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3263
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggu       60

ugggccguua cagacacuga uuauaaacuu cugaaggcua acagaaaaga cuuguggggc      120

augaggugcu uugaaacggc agagaccgcc gaagugcggc gcuuacaccg aggggcgcgu      180

agaaggauug agcggcgcaa gaagagaaua aaacugcuac aggaacucuu cucccaagag      240

aucgcuaaga cagaugaggg auuuuuccag aggaugaaag aaucucccuu uuacgccgag      300

gacaagacua uucugcagga aaauacccuu uucaacgaua aagacuuugc agauaagaca      360

uaucauaagg ccuacccuac caucaaucac cugauuaagg cuuggaucga gaacaaggua      420

aaaccagacc cgcgguugcu cuaucuggca ugucauaaua uaaucaagaa acgcggccac      480

uuccuguuug agggugauuu cgacucagaa aaccaguuug auacaaguau ucaagcccuc      540

uucgaguacu uacgagaaga cauggaggug gacaucgaug cugacagcca gaaggucaaa      600

gaaauucuga aggauuccuc ucuuaaaaau ucggagaaac agucaagauu gaacaagauc      660

cuggggcuaa aacccagcga caagcagaaa aaggccauaa cuaaucucau uaguggaaac      720

aagaucaauu uugcggaucu guaugacaac ccugaucuua aagacgcaga gaaaaauucc      780

auuucuuuca gcaaagauga cuucgaugcc cuguccgacg acuuggcuuc aauccucggc      840

gauaguuuug aacuguuacu gaaggccaaa gcaguguaca acugcagcgu ucucucuaag      900

gugaucggcg acgagcaaua ccuuuccuuc gcuaagguca aaauuuauga aaagcauaaa      960

accgaucuga cgaaguugaa aaaugugaua aagaagcacu uuccaaaaga cuacaagaaa     1020

guauucgggu auaacaagaa cgagaaaaau aacaauaacu acucaggaua uguuggugug     1080

uguaagacua aaagcaagaa gcuaaucauu aauaacagug ucaaucagga agauuuuuac     1140

aaauuccuga agaccauccu cucugccaaa uccgagauaa aggaggugaa cgacauucug     1200

acagaaaucg agacuggcac cuuucuuccc aaacagauca gcaagucgaa ugcggaaauu     1260

ccuuaucagc ugaggaagau ggaguuggag aaaauccucu cuaacgccga aaagcacuuc     1320

uccuuucuga aacaaaagga ugagaaaggg uuaucacaua gugaaaagau uauaaugcug     1380

cuaacauuca agaucccaua cuacauugga cccaucaaug acaaccacaa aaaguuuuuc     1440

ccugaucggu gcuggguggu caaaaaggag aaaagcccau ccggcaagac cacgccgugg     1500

aacuuuuucg accauaucga uaaggaaaag acugcagagg cuuucauuac aucucguacc     1560

aauuuuugua cuuaucucgu uggugagagc gugcugccca agucaagucu uuuguacucc     1620

gaauauacag uacugaauga gauaaacaac cuccagauca uuaucgacgg gaaaaauauu     1680

ugcgauauca agcugaaaca gaagauauac gaagaccuuu ucaaaaagua uaagaaaauc     1740

acccaaaagc agauuagcac guuuaucaaa cacgagggaa uuuguaacaa gaccgacgaa     1800

gugaucauac ugggcauuga uaaagagugc acaucuucau ugaagaguua caucgagcuc     1860

aagaauauuu ucggcaaaca ggucgacgaa aucagcacua agaacaugcu ggaggaaaua     1920

auccgcuggg ccaccauuua ugaugagggg gaaggaaaaa caaucuuaaa gacuaaaauu     1980

aaggcugagu acgguaagua cuguuccgac gagcagauca aaaagauacu gaaucuaaaa     2040

uuuucuggcu gggggagacu cucgaggaag uuccuggaaa ccgugacaag cgagaugccu     2100

ggauuuuccg aaccaguuaa cauuaucacc gccaugcggg agacucaaaa uaaccuuaug     2160

gaauugcugu caagugaguu cacguuuaca gagaauauca aaaagauuaa cucuggcuuc     2220

gaagaugcag agaagcaguu uagcuaugac ggucucguga aaccccuguu cuuauccccu     2280

ucagucaaga aaaugcugug gcagacccuu aaguugguga aagaaaucag ccacauuacu     2340

caggccccac ccaagaagau auuuaucgag auggcuaaag gggcggagcu cgaaccggca     2400

cgaaccaaga cacgccugaa aauucuacaa gaucuguaca auaacugcaa gaaugacgcc     2460

gaugcuuucu cuuccgagau caaagaccuc aguggcaaga uugaaaacga ggauaaucug     2520

agacuuagga gcgacaaauu guaucuguac uauacccagu uaggaaagug uauguacugc     2580

ggcaagccua ucgaaauagg gcauguauuc gauacuucua acuaugacau cgaccacauu     2640

uacccccagu ccaaaaucaa ggaugacuca auuaguaacc ggguucucgu gugcagcucg     2700

uguaauaaaa acaaggagga uaaauaccca cugaaguccg agauccaguc uaagcaacgc     2760

ggauuuugga auuuccuuca gagaaacaau uuuauaagcc uggaaaaauu gaacaggcuc     2820

acacgugcca cgccuauuuc agacgaugag accgcaaagu ucaucgcccg gcagcugguc     2880

gaaacucgac aggcuacaaa aguggccgca aagguccuag agaaaauguu ucccgaaacc     2940

aagaucgugu auaguaaggc ggagacaguu uccauguuca gaaauaaauu ugacauugug     3000

aagugcaggg agauaaacga uuuccaucac gcucaugacg ccuaccugaa uaucguaguc     3060

gguaacgugu auaauacuaa auuuaccaac aauccaugga acuucauuaa ggaaaaacgc     3120

gauaauccca agaucgcaga cacguacaac uauuacaagg uuuuugauua ugacgugaaa     3180

cggaacaaua uuaccgccug ggagaagggc aaaacaauca uaacugucaa ggacaugcuc     3240

aaaagaaaca ccccuaucua cacaaggcaa gcugccugua agaaagggga acuguucaau     3300

cagacuauua ugaagaaggg acuuggccag cacccauuga aaaaggaggg uccguucucu     3360

aacaucagca aauacggggg cuauaauaag guguccgcag cuuacuauac ccugauugaa     3420

uacgaggaga aaggaaacaa gauccgcuca uuagaaacga uaccccucua ucugguaaag     3480

gauauucaaa aagaccagga ugugcuuaag agcuaccuga cagaccuacu cggcaaaaag     3540

gaguuuaaaa uccugguucc uaagaucaag auuaauaguu ugcugaaaau caacggguuc     3600

ccaugccaca uaaccggaaa gacuaaugau uccuuuuuac uccgacccgc cguccaguuc     3660

uguugcucua acaaugaagu gcuguauuuu aaaaagauua uccgguucag cgagauucgu     3720

ucgcagagag agaaaaucgg uaagacaaua ucaccuuacg aagaccuuuc uuuuaggagu     3780

uacauuaagg agaaccugug gaaaaagacc aaaaaugaug aaaucggcga gaaggaguuc     3840

uaugacuugc uccagaaaaa gaaccuggaa aucuacgaua ugcuacugac uaaacauaag     3900

gacaccauuu auaagaaacg cccaaauucc gcgacaaucg auauucuugu gaaggggaaa     3960

gagaaguuua aaagccucau aaucgaaaac caauucgagg ucauucugga aaucuugaag     4020

cuguuuucug ccacgcggaa uguuagcgac uuacagcaca ucggaggcuc caaguacuca     4080

gguguggcua aaauugggaa caagauaagu agccucgaua acuguauccu gauuuaucag     4140

ucuaucaccg gcauuuucga gaaaagaauc gaccuucuga agguguga                  4188


<210>  3264
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3264
augaacgcca auagagagau ggcuuacuau aucggcauug acauggggac cggaagcgug       60

gguauggcaa uaacagauga aaacuacaau cugcucaagg ucaaaggcca ggacuauugg      120

uucguuaggg aguuugaaac ugccaacacg caaaucaagc ggcgcaugca ccgaauuucc      180

aaacguagac uuaggcggca ucaggugcgc guagcgcuga ucagaucuua cuucgcugag      240

gaaauuuuga agaaugaucc cuuauuuuuc aucaggcagg acaacucaau auauuaccug      300

gaggauaaag acagugugcu aaagaccaag gauugccucu uugccgacua uaauuaccac      360

gauaaaaacu acuauaagga cuacccuaca aucuuccauc ugcggcaggc auuuauuaaa      420

gauacuguca aggacgauga gaaucacacc cgcuaugccc gacuucugua cuuggcuauc      480

auuaacaugu ucgaacauag agggcacuuu cuccugaaua gcguguccga ggacaucaac      540

aaagaaaugg uuaaggacgc aggagagggc uucacacugu aucuccaaga gaggauaaag      600

gaaauuaaac cagugucuua cucggauauc uuagacgucc ugguggagaa gaauauuuca      660

cggagcgaaa agaaggagcg uaucguagaa cuuuuuggua ucaaaaagag uuguaaaaac      720

gggauggaga uuauaaagug cuugugugga cuggaggcca auguugcuau cauguucaac      780

cuagaugaca uugaaaacaa gcucaccauc guggucucca aagaugacua ugaggauaag      840

cuggccgaag ugcagccgcu uguggacgag gaaucuuuug agcuguugag ccgcauaaaa      900

cagcucuacg auuacuccca gcugcaauca auuuuaaagg gcuauaaaga gaaggaagac      960

ggcgaggaua aguaccugag ugacgucaga guugaacucu augagaaaca caaggcggac     1020

cuuaaacugu ugaagaaagu guacaagagg gaucuagaca agaaagggaa gaucugcgaa     1080

ggaaagaaua uccagcggag cucuaaguau aacuucaugu uccgauccgg uaaagauggc     1140

acuuacucag cauauacaaa uagccugaac aguuuuuguu cuaagaccau ugagaauaag     1200

uaccgcagaa acaugaaaaa uagggaccgg gccucccucu acgauacgau caagaaagac     1260

cugagcggga uugagaacga ugacguaaag aaaauacuuc gcgauaucga aacugagaag     1320

uuccugccca aacagauuac cggagaaaau ggcgugaucc cuaaccagau ccaugcuaag     1380

gaguugaagg ccauucucaa aaaugcagag aagcaucugc cauuuuuaac agaaaaagac     1440

gauucggguc ugacugucuc ugagagaaua aucucccuau ucagguauug cauucccuac     1500

uauaucgggc cugugggaaa ggacucacgu accggcuggg cuaguauuaa agaagagggc     1560

caaaucuuuc cauggaacau agaacagaag aucgauguug acaagacaag cgaggaguuc     1620

auuacccggc ucaucaaaac guguacuuac cuguccaaug aaaagguggu ccccaaacag     1680

ucucuugugu augagcgaua cugcguauug aacgaaauua auaacaucaa gauaagaggg     1740

aaaaggauug acccucaacu gaagcaggag aucuuuaagg aacucuauau uggaagcaaa     1800

ugugguaaga aaguuacacg caagaaccug ugccaguacc uugugaauaa aggcgccauc     1860

gaggaggauu cagaaauaag ugggaucgac aaggaucuga acaccuacau guccagcuau     1920

ggaaagaugu accagauuuu cggcgacaaa aucaagcggg augacauuaa agaggucgcg     1980

gaagagauca uauauuacgg uacuauuuau gggggcgcca aggaaauguu uaaaaagaaa     2040

aucgagcaau acgugucuga ggauaucuug gacgaagcac agaugaauag gauucucgga     2100

uucaaguuua aggauugggc uagacugagu aaaaaguucu uauaucugga gggcuacgac     2160

aaagggaacc agaaucagcu aacaacccuc augcaagccc ugugggauua caaccuuaau     2220

acgauggaau ugaugaacuc accaauguuu accuucaagg agacacugga gaaacagaag     2280

aauaagaugg aaaaaguccu cugugacuuc gaguaugaag aucuggacga guacuauuuu     2340

agcgcuccgg ugaagcgcau gguuuggcag acuaucgccu gcuuaaaaga agugguacag     2400

auuaugggac acaaccccaa gcgaauauuc aucgagaugc cucgggcaga cgaggaaaaa     2460

ggugagcaag gccguaaggc cuccagaaag aauaggcugc uuucuuugua cgaagagauu     2520

gaagaugagc gcccauggaa aaaggagauc gaaaacgcug cggagucggg gcggcucaau     2580

agcaaaagaa uguaucugua cuaucuacag augggaaagg acgcauacac cggcgaagau     2640

auugacaucg aggaucuguu ugacaacucc aaauacgaua uagaccacau cuaucccagg     2700

ucacucacag augacaauag ucuggaaaac aaccuugucu uggugucugc cacuauuaau     2760

caggauguua agaagaacga cuacccuauc ccauaugaca aaauucgagg uaauagcaag     2820

gugcgcgagc uguggcgguu cuuacacgag aaaaaguuua ugaacgauga aaaauacaau     2880

agacucaugu ccgacguccc ccugacccag gagcaacuug cuggguauau cgccaggcag     2940

cugguggaaa cagcacaggg caccaaggga auagccgaua uuaucaaguc aguaaucccg     3000

gacgcugaua uuauauacag caaagccaag aacgugucug acuuccguaa acgcuauggc     3060

auguugaagu cccggcucac uaaugauuuu caucacgcaa aagacgcgua ccugaacguu     3120

gucgugggga auguguacuu cacgaaguuu acacaaaacc cuagaaaaua uaucaaggau     3180

gagaaugaca uugagaacaa gaaauacaau uauaaccuaa guaagaugua cgauaaagac     3240

gucaucagga auggaaaggu ugcuuggauu gcccagaacg guaaaaaggc aggcaccauc     3300

cagacuauaa agaaagugau ggaucgaaac acccccauca uuacacggca ggccuucgaa     3360

caacgcgggg agcuguauga ccuccagcca aucagcaagc auaaagcuaa gaaagaaaau     3420

uacgugccua uuaaggccac caacgagaag augaaagacg uaacuaagua uggaggcuuu     3480

acaucucuga aucccgcaua cuucgucuuu guggaauacg guuccgcuaa aaagagacag     3540

aggugcuucg ccguugugca cucauauugg gcgaguagca ucaaaaacaa ugaggaucuu     3600

gucacgcauu ugaaggaccg gggguacgag aacccaauag ugauugccga uaagaucaaa     3660

aagaacucgc ugaucaaaua uaauggcuac uucuuauaua uuguuggaau ggacgcucga     3720

aaacaggugg aauuuuccaa ugcaaccucu augugucuca acaauaacuu cauccaauac     3780

auacagaaaa uugagaaggc caauacucug aucgcugaag ccaaaaagag acgccccuau     3840

auugaugaga gcaucacagc agaaaggaac auagcgcuuu acaaugagcu gaagaaaaag     3900

caccuagacu caauuuuuaa aaaccacccu aagggcaucg ggaguguacu cgaguccgga     3960

gaaggcgccu ucguccagcu gucuuuggag cagcaaaucg guauucugua cagcaucauu     4020

caguuuuccu cauucaaaaa ggggaccuuu agcuuaacac ucauaggagg cccaaaggaa     4080

gugggucgua uccggauuag uggcaauaug ccgaaagcua gagagcugaa acuuauccau     4140

uauucuauca cugggaugua caaaaaggaa auuaaccugu uggauuga                  4188


<210>  3265
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3265
auggagaccc aggccagcaa ccaacugauc uccucucacc ucaagggcua ccccauuaaa       60

gacuauuucg uggggcuuga uauaggaaca ucaagugucg guugggcugu uacuaauaag      120

gcauacgaac uguugaaauu uagaagccau aagauguggg gcuccagguu auucgacgag      180

ggggaaucug ccguggcgcg gcgcggauuu cgaucgaugc guagaaggcu ggagcggcgc      240

aaacuaagac ucaagcugcu ugaagaacug uucgcugaug ccauggcaca gguagacccu      300

acguuuuuca ugagguugcg ggagucaaag uaucacuacg aagauaaaac cacaggccau      360

agcaguaagc acauccucuu uauugacaaa aacuauacug aucaggacua cuauaaggag      420

uacccaacca ucuaccaucu gcgcguggcc cugaugaaau ccgguacaga ugacauucga      480

aaacucuucu uagcugucca ccacauccug aaguauagag ggaauuuucu uuacgaagga      540

gcaaccuucg auucuaacgc cagcacuuug gacgaugugc ugaaacaggc ucuagagaau      600

auaacauuua acugcuucga cuccucagcc gcgaucggca guauuggcca aauccucaug      660

gagcugggga agaccaaaag cgacaaggca aaagccauug aaaggcuugu ugauacguau      720

aucauaacug acgaggugga uaccucugga aagacacaga aagaacaggu caaggaggac      780

aagaaacggc ugaaguccuu ugcuaauuug gugcucgguc ugucagccag cuuaacugau      840

cuguucggca guguagaaga ccucgaugag gaccuuaaaa agcugcagau uaucggggau      900

accuacgacg agcgucgcga ugacuuggca aaaguuuggg gagacgaaau ucauaucauc      960

gaugacugua agucugugua ugaugcuauu auacuaaugu ccaucaagga gccgggccug     1020

acaauuagcg aaucgaaagu caaggccuuc aaaaagcaca aagacgaucu cgcgauccug     1080

aagucucuuc ugaaguccga cagaucaaua uacaacacca uguuuaaagu ggaugagaag     1140

gguuugcaua auuaugugca cuacauuaaa caagggagga cggaagagac uucaugcaac     1200

cgggaggacu ucuacaagua uacaaagaag gucguugaag gacuccccga uagcaaggac     1260

aaagaguaca uccuguccga aaucgaguua cagacccugc uaccucucca gcgaauuaag     1320

gauaauggcg ugaucccaua ucagcugcac cuugaagagu ugaaaguaau ucuggccaag     1380

uguggcccca aauuuccuuu ccucaacgag guggcagacg gguuuucugu cgcugaaaag     1440

cugauaaaaa ugcuugaguu ccgcauccca uacuaugugg gaccccugaa uacucaucac     1500

aacguugaua augguggcuu ugccugggcu gugagaaagg ccagcgggag ggucacaccu     1560

uggaacuucg acgacaagau ugaucgggaa aaaucagcag ccgcuuuuau caagaacuug     1620

accaauaaau gcacguaccu ccugggagag gacguguuac caaagaguuc ccugcuauau     1680

agcgaguuca ugcuccugaa cgaacuuaau aacguacgca ucgauggcaa accguuggag     1740

aaggcgguua aggaacaucu gauugaggca guguuuaaac aagaccacaa gaaaaugacc     1800

aagaauagaa uagaacaguu ccucaaagau aacaauuaca uccccgagaa gcauaagccu     1860

gagauuacag gucuggacgg ggaaaucaaa aacgauuuag ccucuuacag ggacaugguc     1920

cguauucugg gcaauaacuu ugaucuuuca auggcugagg aaaucauaac ugacaucacc     1980

auuuucggag agaguaagaa aauguugcgg gaaacacucc gaaauaaguu cagcuccaga     2040

cuggaugacg aggccaucaa aaagcuaucu aagcugaggu aucgcgauug gggccggcuc     2100

ucgagaaaac ugcuuaacgu gauugagggg ugugacaagg caggagacgg uacuccagaa     2160

accaucauaa uuuugaugag gaauuuuagc uacaaccuga uggaguuacu cggcgauaaa     2220

uucuccuuua uggaacgcau ccaggagauu aaugccaagc ugaucgaagg gcaaauaguc     2280

aacccccacg acaucauuga ugaccuugcu cugaguccug ccgugaaacg agcaguuugg     2340

caggcguugc ggaucgugga ugagauugua cacaucaaga aaacacuccc agcucguaua     2400

uucgucgagg ugaccagaag uaacaaggcc gaaaagaaaa agaaagacuc uaggcagaag     2460

cgccugagcg aucuauaugc agccauuaaa aaggacgaug cucugcucuc cggacugacu     2520

aaugaggaau uuggcggucu uaagucaggg uuggccaaau acgacgaugc agcucugcgg     2580

agcaagaaau uauaucucua cuauacgcag augggcagau gcgccuacac aggagagguu     2640

aucgaccugu cucaacuuaa caccgacaau uacgauaucg accauauuua ucccaggucc     2700

cugacuaagg augacaguuu cgauaaccua gugcucugua aacgaaccgc gaaugcccaa     2760

aagagcgaca cauacccgau cucuaaggag auucagaaag cucagaaggc auuuuggaua     2820

uuccugaaac agcagggcuu gaucuccgaa cgcaaguaug agcggcugac cagaauuacu     2880

ccuuuaacag ccgaugaccu cucaggguuu aucgcuaggc agcuggucga aacgaaccag     2940

agugugaaag ccgcaaccac ucuucugcgu cgcuuguacc ccggaguaga ugugguuuuc     3000

gucaaggcgg agaaugugac agacuuucgg cacgauaacg acuucauuaa ggugagaagc     3060

cucaaucauc accaccaugc caaagaugcu uaucugaaca ucgucguugg uaauguguac     3120

cacgaaaggu uuacccgaaa cuuccgggcc uucuuuaaga aaaauggcgc aaaccgcaca     3180

uauaaucuag cuaagauguu caacuacgac gugaaaugca cuaacgccaa ggacgggaag     3240

gcaugggccg uaaaaaccuc gauggauacg gucaagaaaa ugauggacuc caaugaugug     3300

agaguuacca agagggugua cgagcaaaaa ggacagcugu cugacauaaa ccuuacaaag     3360

gcuagcaaag ucaaggcgga uaucuaucuc ccaauuaagg ccaaugaccc uaucauguca     3420

cagaacauga aauacggcgg uuauagugau auuaagaaug cauacuauac uaucauagag     3480

uacuccgaca aaaagaacaa uucuauuaag gcuuuuaucc ccaucccaau uuucauagcc     3540

accaagacac ugacuaagca ggccuugcug gaauauauca aaaccguguu acccaaggcu     3600

aacaaucucc ggauuaucua caaaaagguu uacaacgauc aacugcuuaa aauugacggg     3660

uuuaauuauu accugggcgg acgaagcaac gauagauucu guaucgacaa ugcaauacag     3720

uugaucgugc cucucgagau ucaguuuuau aucaagcugg uagaaaaguu cacggccauu     3780

aucuccgaga acaaaaauau agcgcuaaag gcuucaagcg ucaaaagucg caacccaauu     3840

aacaaucaag uggaaaacau cacacuggag uacacccaga aacuuuuuga uuaucucgug     3900

ucuaaauuca augagcugau cuacuugaag augaggggca acaaguuaac ugacgucaau     3960

aacauugggc guaaacaguu uaucaagcug ucccucgagg agcggugcaa agcccuggca     4020

gaacuucuga auauauucac aaacaugaau aagaaauuga gccuaucgac cauuaacguu     4080

ucagccucua gaaagguggu aaauuucaaa aucaguuccc uggacgaguu uaagauugug     4140

aacgaaagca ucacuggacu cuauucucau gagauaacca uugucuga                  4188


<210>  3266
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3266
augaaccuga gaaauaccac agacgaguac aacaucggcc ucgauauggg gacuaauagc       60

gugggauggg ccgucacgga caagguuggu gcuauugcac acuucaaagg caugcccacc      120

cuugggucca ggcuguuuga ugccgcgcag acagcugccg aagcacgggc ccaucgcgga      180

caacgacgua gauaugugag gcggcgcugg agauuggacu uacugcaguc ucuauuccag      240

gaugagauag acaagguaga uucagaauuu uucaucaggc uccggcagag ucgccugcac      300

guggaggacc gagaagaugg ccaugcugac uacaaauggc cuauuuuuaa cgauagcgac      360

uucacugagg ucgauuauua caagagauuu ccaaccaucu aucaccuuag gaaauggcug      420

auugagacag accaaaaggc agacauccgg uugauauacc ucgccgugca uaauaucguu      480

aagcaccgug guaacuuccu gcgcgaaaau aacaaaaauc uguccucuag aaacgcugau      540

ccggccgagg cgguggggca ccucagguua gcacugaaua acuggugccu ugaacgggga      600

uaugagugug ccuggaaguc gggcggcaau gacaacgcua uuuugaaugu ccugaucgau      660

ggggagaaac gacgcucaga ccaggugaga gaaauugcca agcuaaccgg aaucgauugc      720

ggugacgcaa aacagagcag gcaguguaac gaggcuauag ccaaggcgau uguaggccuc      780

aaggccgaaa ugaaaaacau cuuuggggag uuccccgcag aaaagacucu gauuuaccuu      840

aguuccgaug acgauguuga ggugguccgg gacgcuugcc cugaugacuc uguggagcug      900

uuucaagccu ugugugaacu cuacgcugcc uauauccugc agggauuacu gagcuacgca      960

ccaggccaga caaucuccgu gaauaugguc aaaaaguaug accgcuacgg ugaggaucuc     1020

caucuucuga aagaauugac cagaacguau gccucaaaga gguacgacgc uuucuuucgu     1080

ggggagacuu augaaggaag uagcgauuac guuguggaca aagcgcaggg cuacacccgg     1140

uauaaccuag gcacaacuca acugucuuac gaugacuucc agaaggcagu aaaggaacuc     1200

uucaaaggga ccgaugcccu gcaggacgag cgauaucaag gaaugauggu ggcuuuuggu     1260

gaacagagau uccuuaggcg ccugaagaca uccgauaaug gcucaauuua cuaucaguug     1320

caccucgagg aacugcaugc cauauuagag aaccagggga aacacuaccc cuuucugcua     1380

gcagaagagg acaagaucga gagccucguc aguuuccgga uuccuuauua cgugggacca     1440

cugaccagag aaaaugccgc uguuggccac gaugguaaag agagguuugc cuggucuucc     1500

cgccgagacg ggauggaaca ugcagugauc acgcccugga acugggagga gauaauugau     1560

cgggacaaga gcgcggaaga cuucaucauc cguaugacug gcgauugcac auaccuucaa     1620

ggagagccug ucuugccaaa guguucgcug cucuaugaag aguuuugcgc ucugaaugaa     1680

cuuaacggcg cccacuggac cauugacgag gaugacgaga gaagguucga ugcagcccag     1740

cgcgaacgga ucauguguga ccuguuuaga ucuacuagga aagugacaua caagaaagua     1800

uccgauuggu uggagcgaga agacucauuc guuggggcuc gcgugcaugg aggucagggc     1860

gagcacgggu uugaaaguca gcucggaagc uauauuuucu uuugcaagga ugucuucaaa     1920

accgacuccc uggaugaggc cgacuacccg augauagagc aaaucauuuu auggucuacg     1980

cuguucgaag aucgggacau ccuaagaagg aagaucgagg aagcauaugg cgaccgucuc     2040

accacagagc agauuaaggu gauauguaaa aagcgcuuug cugguugggg gcggcugagc     2100

aaaaaguugc uuacuggcau caaagucgau accgacacag gaagaaaguc aauuaucgau     2160

gugcugaggg aaggcaaucc caacgacggg cagcgaaguc ggacuauggu uuucauggag     2220

gugcuccgcg augagggacu ggguuuucag aaggccguag acgauuucaa uagagacuac     2280

uuuuccgaac auaacaaugg cuuaauggau gucaacgacc ugccugggag cccagcgauu     2340

aggcggucua ucaaucaagc cgugcgaaua guugaugaga ucgugucaau ugcuaaaaag     2400

gaacccgcaa acaucuucau ugaggucacc agagaggaag acgacccucg caaaaaggga     2460

aaaaggacaa agcgucggua ugaugccauc aaaagugcuc uugagguguu uaagaaggac     2520

gauccacagu uguggaaaga acucaccaag aaagacagca acaauaugga ugagagacug     2580

ucccuauacu ucaugcagag gggcaagugc auguacucug gucgccccau aaaaauugag     2640

gagcugcaca cugggaagua ugagguagac cacaucauuc cgcgaucgua caucaaggau     2700

gacagccucg aaaacaaagu gcugguuuau agagaggaaa aucagcauaa gacggauaca     2760

cuuuugauag acuccucaau ccggaggaaa augggcaagu acuggcgcau gcugcacaac     2820

gccaaauuaa uuggagauaa gaaguuucgu aaucucuuca gagaccauau cgaugacaaa     2880

accaugaagg gcuuugucgc acggcaaauu guggagacuu cacagauggu gaaacugguc     2940

cagacccuuc uggaagcgag guauccuggg gcccgcaucg uucccgugaa ggcuaacaua     3000

ucucacgacu ugcgagaugc cgcaggacuc gugaaaugca gagaggcuaa ugacuuccac     3060

caugcccacg augcauuucu ggccugucgg guaggccuau ucauucaaaa gaggcaucca     3120

agcgucuacg acaacccuau cggucuggcu cacaucaugc gcgauuaugc gagagcccag     3180

gcaagggagu acaagaaauc ccauuaccuc gcucacacaa agggguucau ugugaauagu     3240

uuucugagcu cuggauucga caaagaaacc ggcgagguuu uuaaggauga cugggaugcc     3300

gaagccgagg uggaagguau ccggcguguc cuuaacuauc gccagugcuu cauuucccga     3360

augcccgugg aggcuacugg cacauuuugg gaccagacga uauacagucc aaaauguggg     3420

aagaaauuga cccugcccuu aaagagagga cucgauccug acagguaugg cagcuacucu     3480

cgggagcaau ucgcauacuu uuucaucuau gaagcccgcg aucugaagca cgagagacau     3540

auucuucagu uugcgcaggu uccagugagg guagcugccc acaucgcaaa agacccgucc     3600

cagcuggaau cauaugccgu caagcuagcu gggcaagcaa aucucgccuu cgugcggauu     3660

gugcgaagac gcauccugaa aaagcaguug cuggaguuaa acggagacag gcucauaauc     3720

acuggcgucc gggagguucg uaaugcgaca gaauuugcuu ucagucugga ugaggaaacc     3780

cuuauugaga gaugcgugca gaucgccaaa guagguagcc ugauuggggu ggucaacgac     3840

gaagaugagg agcaguuguc ggcagaccuc aaugaacugu ucuccucucu agugaagagg     3900

ggacccaagc uguguaaccg ccuuuacgcc caacucaaac uggauaagua cuugagcaaa     3960

uuugacgagc ugacauuaac ugauaaggcu aaaguugugc uccagcugau cgccauaacc     4020

aacggcucaa uuaauauggc agaccuuagu gcucuggguu ccucuaagua ugccggcugc     4080

augcagguca gcuacuucaa gcaauugaac gcgccuaucg gggaagugaa uaucauugau     4140

caguccguua cgggaauguu ugagcggcga acccauauag gccucuga                  4188


<210>  3267
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3267
augaaggaga ucaaagacua cauuauaggc cuggauaucg ggaccaacag cugcggauau       60

guggucacag acaagcagaa uaacauucuc aaacuuaagg guaaaacugc caucggcgcu      120

agacuguuca aggaagggca agcagccgcg gauaggcggu ccuuucgcac gacccgacgu      180

agauuggcua ggcggcacug gcgcuuagga cugcuagagg aaauuuucga ccccgagaug      240

gaaaagaucg auccuaauuu cuucagaagg cucaaagagu cugacuacuc accaaaggau      300

agucggaaac aguuuaacgc cauaguuuuc aagagcauca aagcagacaa gcgcuuuuau      360

aagaaauacc cgacaauuua ucaucugcga aaugcccuua ugcacgauaa ccagaagcau      420

gaccugagag agaucuucau ggcugugcac cauauuguaa aauacagggg caacuuuuug      480

cgggaagauu ccgugaaugc auucaaggcc gcuaaauuuu cucuccgugg ugaggacggg      540

aucggacccg ucgauaagcu gaaugaccug cucaaagaaa uauauucgga guacucaccu      600

gaguuagcca cuaacaaucu gagcaagauu gaagacauca uuaaggauaa aaaccuuuac      660

aagcaggaca aauugaagca aguggcgacc cugcuaauca aagaggcaga uaguaaggac      720

aaggccaaac ucaauaagga uaucgcuaaa cagguugcca acuccuucau gggcuauaug      780

uuucgccugg acacacuuuu cucucugacc gauguggacg ucaaggauua caaauugaag      840

uucagcgacg caaauauuga ugaaucccuc gacgaccuga cuucauuacu gacagaugcu      900

cagauagagu uugugcucga acuucagagu aucuauaaca ccauuguacu gaacgagauc      960

guuccagacg gcaugagcuu gucugagucc augauaaaga aauacgauga ccacaaggaa     1020

gaucuaaaaa uuuucaagga guauaucgac ucacugagcg aucagaaaaa ggccaagaaa     1080

cuccugagug cguacaaucu uuaugugaac uacagaaagu cugaccuggu cgaagccaaa     1140

aauuuguuua agaacaaaaa ggugggggau aaaaauuucg cagacgugau cuccaacuuu     1200

gaggucuucg gaaaguuugu uagcgauaau cucgacgauu cggaacuggc uaacaagauu     1260

aaagccaggu uagaccuggg ugaguuccua cccaagcagc ggacgaauca gaacggcgug     1320

aucccuuacc agcuccacca aguagagcug acucagauuc uugaaaaaca ggggaaguau     1380

uacccauuuu ugauaacccc caauccugug gagucucauc gaaacaaugc uccauaugaa     1440

aucuccgagc uggucucauu ccgcgugccg uacuauguug gaccccucau ugacaaccaa     1500

aguaucaaag auaagcagaa uaagaacaaa uuugccugga uggugagaca gaagcagggc     1560

caaaucacac cuuggaaccu ggaagagaug gucgacacua ccgagagcgc aaaucaguuc     1620

auuaaaagga ugacacggaa ggauaccuac cuucuggccg aagacguguu gccaaaaucc     1680

ucucucguau aucagaaguu uaugauacug gaugaguuaa accgcaucaa gauugacggu     1740

aaaaagcuga cgagcgaacu aaaacacgau aucuucgaga aacucuucaa aaagcagaag     1800

ucaauuaauc uggacaaccu uaaaaauuac uugcugguug aggggaacau ccccggacuc     1860

auagaaggcc ugagugaugg caucaauuuu aacaauuccu ucagcacuua cauugacuau     1920

agaaacaucu uuggggauga gauugacaau ccuaacaagc aagcugauuu cgaaaagaug     1980

aucgaguggu cuacaguguu ugaagacagg aagauauuca aacguaaguu aaaagagauu     2040

accuggcuga cuucagagca gaucaagcag gucaguagca agcgguacuc cggauggggu     2100

cgacuuucua aaaaguugcu cacacagauu accgacgaaa auggcgugaa cauccugcaa     2160

agacuaugga augagccaga aacgcugacc gagguccucg cgaaccccgu gauaaaaagg     2220

aagaucguug aagcaaacuc gagcuuuaca aaagccaagg auaugaagga cauucuggug     2280

gaggcuuaua cuuccccgca gaauaaaaag gccauccgcc agguaaugcg ggucguggau     2340

gacauuaucg cagccgcuca ugggaaaaag ccuucacaga uagccauuga guucgcaaga     2400

gaagaucaag acaaccccca gaugaccaaa aguaggaagg cgcagcuuga ucaaaucuac     2460

gcuaagaucu cugacgaguu uuuggaugac gccguuaaaa augaacugaa gaacaugaaa     2520

gauaauaagc cauuagcaaa agacaaggug uuccucuauu uuaagcagau gggacgcgac     2580

gccuacagcg gcgagaaacu gucccuugau aaccugcagg acuaugauau ugaccacauc     2640

uacccucagu cauucauuaa ggaugacagc uuugauaauc gagucuuggu gcaacgggcu     2700

uacaacaaug guaaaucuga caacguaccc gugcuccugu ucgggaauaa gauagccgca     2760

ggccuaggaa ucacaauucg ucagaugugg aaaaaguggc uggaacucgg ccugaucucc     2820

aagagaaaau ucaacaaucu uauuacugau ccagagaaga ucaguaggaa cauggcuagc     2880

ggguuuguua aucgccaguu ggucgagacc ucucagguga uaaaacuggu ggccaacauc     2940

uuacaagcga agcuccccga cacagaaauu aucgagguca aagccuccua uaacucaauu     3000

cugcggaagg aauuuaaucu uuacaaaagu agagagguua acgauuauca ccaugcuauc     3060

gacgcauacc ugaccacuau agugggaaau uaucuauacc agguguaucc uaagcucagg     3120

ccauacuuug uauacgguca guucaagaaa uuuggcaagg ccgagaacca ggaagaugac     3180

ccggucaaaa agauuaaaaa guucaauuuu aucuaucaac ugaucugggg gaaggacgau     3240

gcuauuugua uaagccacac gaaccaacag guguucucga aaaaggacau cauugauaaa     3300

uugacaaccg ccuacaauuu uaaguauaug aacaucuccc augcaguuua cacucgaaau     3360

aacaauaugu ucaaacaaac ccuguuuccc auuaucgagc gcacaaccgc gaagacuaag     3420

ucuuuaauac cuaaaaagcu cgaccggcca acagauaucu auggaggcua cagcaacaau     3480

aacgacgccu aucuggcucu ugugaaaguc gauaagaaaa gagguucaga auacagggug     3540

guugggauuc ccaugcgugc cgcagcuaug cugaaggcca guaagaauua cgacgaggau     3600

uugcgcaaag ugaucgaacc uaugguaaug uucgacaaga acggcaaagc aaagcgggga     3660

auugagggcu ucagaguccu cguggggaaa augccauuua agcaggccgu gcuggaugga     3720

aauaaaaagu ucaugcuaac ggguaccguc gacacuccca accugaagca gcuuguucuc     3780

ucccagcaaa acaugaaaua uauccuggau uacauagagg acguggacug caggaagcac     3840

aaauuuggcg auaagguaca gaauccggac aaauguuugc uggaaguguu cgaugagauu     3900

aucgaaaucg ucgacaagua uuuugaguua uucgauucuc gagacuuucg ggaaaagcuc     3960

cgcaacagca gaaauaaauu cauuaagcug gaggcuucca aaaaggcgga uacaauccuu     4020

gccauacuga aaggguugca ggcaaaguca accagggcua caauuaagga gcucaacauc     4080

agcgacuuug gacggauuau caccaguucu auauccccug aagcccagcu gauuuaccaa     4140

agcccaacug gccuauucga gcgaagagug aaaguuaagg aucuguga                  4188


<210>  3268
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3268
augagcaaga aauaccagaa caccaaguau uacgugggcc uggacaucgg gacauccucu       60

gucggauggg ccguuacuga ugcuaauuau gagcuccuua aagugaacgg ucacaagacg      120

uggggcucaa gacuguucac cgaagcagag acagccuugg aaaggcggac ucaucgcgcg      180

acccgacgua gauuacaaag gcggaaacag cgccuggacc uacucgauga gcuguuugcu      240

gaggagcuug ccaagguaga cccccaguuc cuguugagac ucaaggagag ucaguaccac      300

auggaagaua aaagcacaga cgauaaguau gugcuguuua augaccaaaa caggaccgau      360

ggggaguucu uuacugaaua cccuacaauu uaucaucugc gguccgagcu caugcagggg      420

aaaaaggagg acauacgcgc agucuacuua gcccugcacc auaucgugaa auaccgaggc      480

cacuuccuuu ugccagguga uaauuaugac augcagggga accugucuga acuaauuaag      540

agacucuuua aguucaucga ggaaacccug uuuauugagg aucuuggacu gacggacgaa      600

auccagucga aaauagagga gaucuugcuc acuaagacca aaacaaggcu ggaccggcaa      660

aaggaaauug uuaaauuauu caauacuaag caccagaaac uggaugacaa gcagaagaaa      720

cuccuugagg cuaucuuuaa gcugugcuug ggccuaaaag gcaacauggu gaauauuuuc      780

ggggauaagg gagaauacgg uaucgacuuu accgaugucg aggacaaaaa gaagaaagug      840

gaguucucag cagauuauga caacaauaua aacgauuacg uagacuucuu uggcgaauau      900

uucgagcugc ucgaaauuac aaagaaaauc aaugauaccg uuauuuuuag cgagauccug      960

guggacggga agggauucag ugaauccaaa gucaucucuu acgagaagca ucagcaagac     1020

cuucguaagc ugaaaacguu gcucaagagc cuggauaaaa cuuuaggcua uaaguacggu     1080

gaagggcugu accagaaaau ucuacucgac gaugugaagu ccaacuaugu gaauuacuca     1140

ggaaacaguc agaagcugaa aaaguguaca aaagacgagu uuuauggcuu cauaaagagc     1200

aaacuuaacu cugucaccaa gaaagagaag guuuccgaag cccaacagaa ggagaucgcu     1260

cagauuuugg ccgauaugga auuuggcgac uaccugccgc uccagcgcac uacagauaau     1320

ggggugaucc ccaaccaacu gcacaaaaag gagcuuauag agauuaucga acagcagacc     1380

aaauaucaug acuucuuuaa ugauguagac gagaacggac ugacgaucaa ggauaaaauu     1440

aucucauuga ugaccuuccg gauuccuuac uauguggguc cacucaauga cuaccacaag     1500

gucgcggaug gcacagggca cgcauggaua gaaaagaaaa aggacggaaa aaucuacccc     1560

uggaacuuug acgagauggu ggauacugaa gccaccgcug agaaguucau uaaaaaucug     1620

acaaacaagu gcacuuauuu aaagggcgag gacguucugc cuaaagaaag caagcuauac     1680

agugaguuua ugcuccugaa ugaacuuaac aauaucaaaa ucgauaacca gaaguuggac     1740

ccaaaacuga agaugaagau ggugucugaa cucuucauga aaaagaauga aaagauuacc     1800

aagaaaaaga uagucgauua ucugaggucc aucggucaag ccaacgggaa ugagauggug     1860

agcggcauug accgggagau caaaucggau uuaaagacaa uucauaagau ccagcacuac     1920

uaucaggacg caucucugga aauggcugag aacauaaucc gauggaacac cauuuucaau     1980

gauuccacuu cagacauccu uaaagauaag auuacgaaag ccuacggaga cguauugaca     2040

cccggccaga ucaaggggcu caguaaccug gcguguaaag auuggggaaa gcuaagcgaa     2100

aaguuucuga ccgguauacg cugcgacguu ggcgaguuca aaaauauuuc caucauucaa     2160

gccaugagag acacuaacaa uaaccucaug gaacugcuuu cuaauaagua ugaguuuaug     2220

gauaaaaucc aggaguacaa caaggaauac uauggggacu ucaccuuuaa agcagagccu     2280

agcauaaugg augacuugua cgugggacca ggcccgaaga gggcuaucug gcaggccguc     2340

cggauugugg aagagaucaa gcgcauuaug ggucaugauc ccgagaaaau cuuccuggaa     2400

acaaccagaa cuaauagggc uccuaagguc aaaaacacaa agcguuauga cgccuuagag     2460

aaaaaguacg aaucagugaa ggagcacgaa uaugcagagg uuaaaaagga guuugaaaua     2520

gagaaaaaug auagucucaa cauuaagaaa cuguuccuuu acuuuacgca gcuggggcau     2580

uguauguauu ccgacgaaaa gaucccauac aaugaguugu ucaccaaccg guacgauauc     2640

gaccacauuu auccccgaag caagacuaga gauaauucua uggacaaccu cgugcuggua     2700

aaagccgaau gcaacaggga caagaaagau aauuacccua ucaagcuaaa aacaaacauu     2760

caacagaagu gucagguccg cugggcuuau cugcgggaca aaaagcucau aaccgaggag     2820

aaguacaaua gacuggugag gaaaacacca cuuacugaag augaguuguc acaauuuauc     2880

gcgcgccagc ugguuuucac cagucagagc gugaaggcaa cggccgaagu cuuuaaacga     2940

uuauaugagg acuccaagau uugcuacgug aaagcugaaa acguaucuga uuuccgguau     3000

ggcucgaccc guaagaugag caaggacgau cagccccaac cguucaucaa aaauagagac     3060

gugaacgauu uucaccaugc caaggacgca uaccucaaua uuguugucgg aaacguguac     3120

gaugugaaau ucacacagaa uccuacuagg uuuaucaagg ccaacaaaga cuauucccug     3180

aauuacauau uccgcuaucc cgucaacaag aagggcgaga auguuuggga uccagagcag     3240

ucaaugaaaa ucguggaaaa gacccuuaaa aguaacgacg ugcggguaac aaagcugguc     3300

aaaccuauua cuucucccau cagcaaugag acccuacaca aggcucucaa guguaaacca     3360

ggguccuacc ugcccagaaa gucaagcaaa ggaacgccuu uggccgacgu gacaaaguau     3420

gguggcuacg agaaaauuaa gaacgcauau uacucuaucu acaaauauac cuacaugaag     3480

aagaaagaau gcauggagga auuucuguua guuccaauac cgauucaucu cgugaacaau     3540

gaugaggaac ugcccaagua uaucaacgac aaaaucgcgg agagggcuaa gaaaaagaaa     3600

cuugaaacug ucgaguguau ugugaaagag cuguaccagg ggacauuggu ucgaauaaau     3660

ggauaugauu accgcaucgc cggcuccagu gguaagcaau ucgaacucca caacgggauu     3720

aaucugaucg uggagccuug gguagaaaaa guccuaaagg agaccgacaa auuugugaga     3780

aagagcaacu cuaagcugga uaaaaagcag cagaaagaaa auacuaccga gacacuuauu     3840

gagaagcagc ucaacgacgu guucgaauau cugugcagaa aauugaauca aggcaucuuu     3900

aagaacaaua agcacacgcu gccagcaaaa uuacucggag auuccauaca gaagagguuc     3960

gaggacgccu caaucgaaga ugaguuugaa gucauuaaag agcugaucag ugcuauugcc     4020

aagaaccccc agaauuucaa aaccauccaa aagguucuug acauaagcgg cucguccauu     4080

ccucguuuca accugaaauu gacuaagaau aacguggaug aguuuaucau caagaaugaa     4140

ucuguaacag gacucuucac ccaugagaaa gugauuuuuc gcaaguga                  4188


<210>  3269
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3269
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggu       60

ugggccguua cagacacuga uuauaaacuu cugaaggcua acagaaaaga cuuguggggc      120

augaggugcu uugaaacggc agagaccgcc gaagugcggc gcuuacaccg aggggcgcgu      180

agaaggauug agcggcgcaa gaagagaaua aaacugcuac aggaacucuu cucccaagag      240

aucgcuaaga uugaugaggg auuuuuccag aggaugaaag aaucuccccu guacgccgag      300

gacaagacaa uccuucagga aaauacucug uuuaacgaua aagacuucac cgauaagaca      360

uaucauaagg cauacccuac cauuaaucac uugaucaaag ccuggauaga gaacaaggua      420

aaaccagacc cgcggcuccu guaucuggcu ugucauaaua ucauuaagaa acgcggccac      480

uuucucuucg agggugauuu ugacucagaa aaccaguucg auacaaguau ccaagcauua      540

uuugaguacc ugcgagaaga cauggagcuu gacauugaug ccgacagcca gaaggugaaa      600

gaaaucuuga aggauuccuc ucugaaaaau ucggagaaac agucaagacu aaacaagaug      660

cucgggcuga aacccagcga caagcagaaa aaggcuauaa cuaaucuuau uaguggaaac      720

aagaucaauu ucgccgaucu guaugacaac ccugauuuga aagacgcgga gaaaaauucc      780

auuucuuuua gcaaagauga cuucgaugca cucuccgacg accuggccuc aaucuuaggc      840

gauaguuucg aacugcuccu uaaggcuaaa gccgucuaca acugcagcgu gcugucuaag      900

guuaucggcg acgagcaaua cuuguccuuu gcaaagguga aaauuuauga aaagcauaaa      960

accgaucuaa cgaagcugaa aaaugucaua aagaagcacu ucccaaaaga cuacaagaaa     1020

guguuugggu auaacaagaa cgagaaaaau aacaauaacu acucagcuua uguaggaguu     1080

uguaagauca cucacaaaaa gcucgugauu aagaauagcg ccagucagga agauuucuac     1140

aaauuucuga agaccauccu uucugcgaaa uccgagauaa aggaggucaa cgacauucug     1200

acagaaaucg agacugguac cuucuugccc aaacagauca gcaagucgaa ugccgaaauu     1260

ccuuaucagc ucaggaagau ggagcuggaa aagaucuuau cuaacgcaga gaagcauuuu     1320

uccuuccuga aacaaaagga ugagaaaggc cuaucacaca gugaaaagau uauaaugcuc     1380

cugacauuua agaucccaua cuacauuggg cccaucaaug acaaccauaa aaagagcuuc     1440

ccugaucggu gcuggguggu gaaaaaggag aaauccccau cuggaaagac cacgccgugg     1500

aacuuuuucg accacaucga uaaggaaaag acugcugagg aguuuauuac aagccguacc     1560

aauuucugua cuuaucuuau aggcgaauca gucuugccca agaguucccu gcucuacagc     1620

gaguauacag uucugaauga aaucaacaau cuucagauua ucauugacgg uaaaaacauc     1680

ugcgauauaa agcugaaaca gaagaucuac gaggaauugu ucaaaaagua uaagaaaauu     1740

acccaaaagc agaucucuac guuuauuaaa cacgagggga ucuguaacaa gaccgacgag     1800

gugauaauuc ucggaaucga caaagaaugc acaucaaguu uaaagagcua cauugaguua     1860

aagaauaucu ucggcaaaca gguagaugaa auauccacua agaacaugcu ggaggaaauc     1920

auucgcuggg ccaccaucua ugacgagggc gaggggaaaa caauucuaaa gacuaaaauc     1980

aaggcugaau acggaaagua cuguucugau gagcagauua aaaagauacu caaucugaaa     2040

uuuucggguu ggggcagacu uagcaggaag uucuuggaaa ccgugacauc cgagaugccu     2100

ggguuuucag aaccagucaa caucaucacc gccaugcggg agacucaaaa uaaccugaug     2160

gagcuccuga guucugaguu caaauuuacg gaaaauauua agaagaucaa cagcggauuc     2220

gaagacgcag agaaacaguu uuccuauaau ggcuuaguga agccccuguu ccuuucaccu     2280

agcguuaaaa agauguugug gcagacacuc aaacugguga aggagauuuc ucauauaacc     2340

caggccccac ccaagaaaau cuuuauugaa auggcuaagg gugcggagcu agaaccggca     2400

cgaacuaaaa cccgccugaa gauccuccaa gaucuguacu ccaguugcaa aaacgacgcc     2460

gaugcuuuca gcucugagau ucaguaucuu uccgggaaga ucgaaaauga ggacaacuug     2520

agacugaggu cagauaaauu auaccucuau uacacacagc ugggcaagug uauguauugc     2580

ggaaagccua uagagaucgg ccacgucuuu gacaccagua auuacgauau ugaccauauc     2640

uacccccaga gcaaaauuaa ggaugacucg aucuccaacc gggugcuugu augcucuagc     2700

uguaauaaaa acaaggaaga caaauaucca cugaaggccg agauacaauc aaagcagcgc     2760

ggguucugga acuucuugca gagaaauaac uuuauuaguc ucgaaaaacu gaauaggcua     2820

acucguacau ccccuaucuc ugaugacgag acggcaaagu ucaucgcccg gcagcugguu     2880

gaaacccgac aagcuacuaa aguggccgca aagguccucg agaaaauguu ucccgagaca     2940

aagauugugu acagcaaggc ggaaaccguc uccauguuca gaaacaaauu ugauauaauc     3000

aagugcaggg agauuaauga cuuccaccau gcucacgaug ccuaucugaa caucgugguu     3060

ggaaaugugu acaacacaaa auuuacuaau aacccaugga auuucauuaa ggaaaaacgc     3120

gacaacccca agaucgcaga uaccuauaau uacuauaagg uauuugacua cgaugucaaa     3180

cggaacaaua uaacggccug ggagaagggu aaaaccauca uuacagugaa ggacaugcuu     3240

aaaagaaaca cuccuaucua caccaggcag gcugccugua agaaaggcga auuguucaac     3300

cagacaauua ugaagaaggg gcugggacaa cacccacuga aaaaggaggg cccgcucuca     3360

aauaucagca aauauggugg guacaacaag guuagugcag cuuauuacac ucugauagag     3420

uaugaagaga aaggcucuaa gauucgcucc cuugaaacca ucccccugua ccuagugaag     3480

gauauccaga aaaauaagga cggacucaaa agcuaucuga cggaccagau uggcaagaaa     3540

gagaucaaga uauugauucc uaagaucaaa auuaacucgc uguuaaagau caauggguuu     3600

ccaugccaua uaacaggaaa aaugggugag gccuucgucc uccgacccgc ggugcaguuc     3660

uguugcucaa acgaugaagc ccuguacuuu aagaaaauua uccgguucaa ugagauccgu     3720

aagcaaagag aaaagauggg caaaaccauu ucuccuuacg aggaccuuag uuuuaggucc     3780

uauaucaagg auaaccugug uaaaaagacu aaagcugacg aaauugagga gaaggaguuc     3840

uacgauuugc uccagaaaaa gaaucuggaa auauaugaca ugcuacugac aaagcacaaa     3900

gauaccaucu acaagaaacg cccaaacagc gcaacuauug acauccuugu aaaggggaaa     3960

gaaaaguuua agucucucau cauugagaau caguucgaag ugauacugga gaucuugaaa     4020

cuguuuagcg ccacccggaa cguuuccgau uuacagcaua uuggaggcuc aaagaaugcu     4080

gguaacgcca gagucgggaa uaaaaucagu agccucgaca acugcauucu gaucuaucaa     4140

ucuauaacag gcaucuucga gaagaggauu gaucuucuga aaaucuga                  4188


<210>  3270
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3270
augagcuuca agaaaauccu gggccucgac gugggggucg ccuccauugg augggcuguu       60

gugagagaga acgaagagaa ucccaacuuu aauaccauaa caaaacuugg uguaagggug      120

gucccuauca cuucugauga aaaacugaac uucgagaagg gcaaaucaau uacgaguaau      180

gcagaacgga ccuuggcccg cagcucccga cguaacuuac agagauuuag gcugcggcgc      240

aagaaucuaa ucgcgauucu ccugcaaaac cagaucauaa caccaacuac cccgcuuaca      300

gagaagggga aauucaccac uuuugagaca cugaagauca gagcuaaagc caccaaggaa      360

aagauugagu uggaggaacu cgcaagggug cugcugcaca ucaauaagaa gcggggauac      420

aaaucuucgc gcaagguuaa aaacucagag gacggcaagg ccauugaugg uauggaagcu      480

gcaaaaaucc ucuaugagaa uaaguuaacg cccgggcagu acacuuauca gcugcuugaa      540

aaaggaagca acaagauacc ugacuucuac ugcagugauu ugcaaauuga gugggaagug      600

aucuggaauu uucagaagca guucuaucca aaccugcuaa ccgaggacuu uaaaaaggag      660

cucaaaggca uuggccagcg agccacagcu gcccuguucc uuaagaaaua cgggaucuau      720

acugcgcuga auaagggaaa gagagaagag aaaaaguugc aagcauacaa auggaggucc      780

aagucuguca gcgagaaaau ccccguggaa gaacucgccu acguaauugc ugagauaaac      840

ucccauauca ccaacucaag ugguuaucug ggcacaauua gcgaucgguc uaaggaguua      900

uacuuuaauc agcagaccau cggggaaaac cuguaugccc agguugcaaa gaauacgcac      960

gcuaaacuca agaaccaagu guuccuucgu caggacuacu ccgccgaguu ugaacagaua     1020

ugggagacuc aagcgaaauu ccauccucag cugacaccag aauugaagaa aaagauucgc     1080

gauucaauca ucuucuauca gagaaagcua aggagccaga aacggcugau uaguaucugu     1140

gaguuugaga aucgagaaau ugagauaauc aagaacggca aaaagaaaaa gaaaaagauu     1200

ggacucaagg ccgcacccaa aucuuccccu cuguuccaag aauuuaagau cuggcagguc     1260

cuuaauaaca ucgugauuaa acaggagggu aaggaccgcc uguugaccug ggaggaaaaa     1320

gagcuccugu ucacugaauu aaauauaaag gggaacauca gcggacagaa ggcucuggcc     1380

cuacucaaac ugucgccaaa ggauacaaaa auuaauguga ccauccuuga gggcaaccac     1440

acgcaagaac aguuguacag agucuaucug aggauucucg gcauggaggg guacgacguu     1500

uuugaucugc uuaagauccc ggagaaucag gacgaaauaa aacccgcuga cucuaagauc     1560

uccgccgcag agauuaagga aaccgugaaa ucaguauuca agacaugcag uaucagcccu     1620

uccauucugg auuuugaccc auugcucccc aacccugagu ucgaaaauca gucuagcuac     1680

caacuguggc auuuacugua uucauacgag ggagauaguu ccccaagcgg uaacgagaaa     1740

cuacucgaac ugcuuaagaa caaauuuggc uucaagaagg agcacgccuu gcugcucgcu     1800

ucuaucucau auacuuacau auaugggaau cugaguagca aagcgaugcg gaagauuuac     1860

cccuauucca aagaaaacac cuacucugag gcauguaugu uagccggaua ccgccacucg     1920

aagaaugcuc ugccgagaga ggaacuugug aaauaucagu ugaaggacag gcucgagauc     1980

cugccuaaaa acagccuacg uaaucccguc guggaaaagg uucugaacca gcucauuaau     2040

cuggugaacu cccuuaugga ugaguacggc gccuuugacg aaauccgguu ggagauggca     2100

cgagagcuga agaaaaaugc caaggaaaga gagaaauggu caaagaacau agcugaugcc     2160

acaaguagga aucagaaaca ugcagaaauc uuaaagaagg acuucaaaau uucuaaccca     2220

agcaagaaug aucucauccg cuaucggcug uaccuugagc ugaaagcgaa cgguuauaau     2280

gacuuguaca cuaacaccua uauugauauc aacuaccucu uuacacacag auacgacaua     2340

gaccauauua ucccucaauc caagguccug gaugacucau ucagcaauaa agugcuagua     2400

cccaggcagg cuaaccugga aaagucuaau cucaccgccu ucgauuauau cucccaccag     2460

gggaaggaga aagaggaaca cuaccugaac guuauuacug agcuuuauaa gcgcggcagu     2520

aucagcgaaa cgaaauacaa gaauuugcag aaaaagggaa acgagauugg cgaaggguuu     2580

auaaaucgag accugaagaa cacacaauau aucgugcgga aagcacguga gauuuuaucu     2640

cucaucaccc caaaugucgu gacuaccaca ggagagauua ccgccaagcu gagaaaagau     2700

uggaaccuua ucaauuccau gaaggaacug aacauggaca aauucaggaa ugcucgccua     2760

acugagcucg ucgaaaacuc acggggugag aagaaggaaa uaaucauuga uuggaauaaa     2820

agaaacgacc agaggcauca cgccaucgau gcacuggugg uugcuuuuac aacgcgaaac     2880

caugugcagu acuugaauua uaugaacgcc cgcgaggacg agagucacaa gcaacauagc     2940

cacauucugu cgaucgagaa aaagauaaaa uacgauaagu uccacgaaaa uggcucccgg     3000

cagucuagau uuauuuaccc caccagcaac uucagggaag aggcgaaaaa gaaguuaaaa     3060

gagauccucg uaucauauaa gacuaaaaau aaggucguga caguuaacau caauaaaccu     3120

aagaagaaag ggggauacag uccaaagcau gaacugaccc cgcguggccc ccuucacaaa     3180

gagacaauuu augguaugau acgcauccug cauacuaagg aaauuaaagu guccucuaag     3240

uugaacuuug agaccgucgu gcggauccag aauccuagau acaggaacgc ccuccuggaa     3300

cgacuaaagg acaauuucaa cgaucccaaa aaggcuuuua gcgggaccaa uucccugacg     3360

aaaaacccag uauuccuuac agcaaauuca acugagaccg ugccugagaa gguuacaauu     3420

cagugguuug aaaaagucua uacuauccgg aagccaauaa accccaccaa uuucaagaac     3480

gagaaaaaca ucgaaaaggu gcucgacccg acgauucgca cacuguugaa aaagagacug     3540

caagaguaca auggcaacgc caaagaggcu uuuagcgacu uagaaaagaa uccuaucugg     3600

cucaaccagg agaaaggaau uugcaucaag accgugacua uaacaggcau uaguaauguc     3660

gaaucucugc acaccaagaa agaucacuuc gggaaggagc uucuggacau caacggaaau     3720

ccaauccccg uugauuucgu guccacuggu aacaaucauc acguggccau uuaugaagac     3780

agcgagggca acuugcagga aaaaguaguc ucguuuuacg aggcagugga gaggguuaau     3840

caggggcucc cugugaucga uacccuguac aaccuagacc ugggauggaa guuccuuuuu     3900

acaaugaaac aaaaugaaau guuccucuuu cccgcggaug acuucgaucc aaacgacaua     3960

gauauuuaug acgagaagaa ucugaucuug auuucaaagc auuuauuucg gguccagaaa     4020

cugucuagua aggauuacug uuuccgacac caucucgaaa cguccaucaa cuuuaacgac     4080

ccugcccuga aaggcauaac cuggagacgc gagagcccca auggucuuag gaaccuggug     4140

aagguucguu ugaaucaccu agggaaaauu gugcacaucg gcgaguga                  4188


<210>  3271
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3271
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggu       60

ugggccguua cagacacuga uuauaaacuu cugaaggcua accacaaaga cuuguggggc      120

augagaugcu uugaaacggc agagaccgcc gaaaagaggc gguuauuccg cggggcgcga      180

cguagaauug agaggcggcg caagagaaua aaacugcuac aggaacucuu uucccaagag      240

aucgcuaaga uugaugagga cuucuuucag aggauggaag agucucccuu cuacgccgaa      300

gauaaaacaa uccugcagga gaauacucuu uuuaacgacc gggauuucac cgacaagaca      360

uaucauaaag cauacccuac cauuaaucac cugaucaagg ccuggauaga gaacaaggug      420

aaaccagauc cgcgcuugcu cuaucuggcu ugucauaaua ucauuaagaa acgaggacac      480

uuucuguucg aaggcgacuu ugauucagag aaccaguucg acacuucugu acaagcacuc      540

uuugaauacu uaagagagga cauggaacug gauaucgacg ccgauagcaa gaaaauuaag      600

gagauccuua aagacuccgc uuugaagaau agugauaagc agaacaggcu gaauaaacua      660

cucggucuga agucgucaga caaacagaag aaagcccuua caaaccugau aagcgggaau      720

aaggcgaacu ucgcagauuu guaugacaau cccgaucuca aggacgccga gaaaaacagu      780

auuuccuucu cuaaggauga cuuugacgcu cugagcgaug aauuagccuc cgugcuggga      840

gacucauucg agcuccuucu gcgggcaaaa gcugucuaca auugcagugu guugagcaag      900

guuaucggcg augaacagua ccuaucuuuu gccaaaguga agauuuauga gaagcauaaa      960

aacgaccuga ccaagcucaa agaagucgug aagaaauacu ucccuaucga guaucguaac     1020

aucuuuggcu acaagcaauc cgagaauaac aauuguaacu auucacgcua cguagggguu     1080

ugcaagagcg gaaguaaaaa gaaagugauu aauaagacgg cgucucagga agauuucuau     1140

aaauuucuga aggacauacu ucugaaaaga uccgaugucc cagaggccaa cgguaucuug     1200

acugaaauug agaccggcac auuccucccc aagcagauca gcaagaauaa cgcagaaaua     1260

ccuuaccagc ugaggaaaau ugaguuagag aagauccuga cuaaugcuga aaaauuuuuc     1320

ucguuucuaa agcaaaaaga cgagaacggg cucucucacu ccgaaaagau cauuaugcug     1380

cuuaccuuca agaucccaua cuauauugga cccauaaaua caucacacaa acaguacuuu     1440

ccugaucgga guugggugau caagaaagag aacgccccac aggagaagau uaccccgugg     1500

aauuucuucg accauaucga uaaagaaaag acggcugagg ccuuuaucac uagccgaaca     1560

aacuucugua ccuauuugau uggcgaaucc gugcugccca aaaauucucu ccuguacagc     1620

gaguauacug uccuuaacga aauaaacaau cugcaaauca uuaucgacgg uaagaacauu     1680

ugcgauguua aguugaaaca gaagaucuac gaggagcucu uuaaaaagua uaagaaagug     1740

acacagaagc agauaucaac cuucaucaaa cacgaaggga uuuguaauaa gacggacgag     1800

guaaucauuc ugggaaucga caaagaaugc accaguuccu uaaagagcua cauagagcug     1860

aaaaacauuu uuggcaagca aguggaugaa aucucuacaa agaauaugcu agaggacauu     1920

auccgcuggg caacuauaua cgaugagggc gaagggaaaa ccauccucaa gacaaaaauu     1980

aaggccgagu augaaaaaua cuauucagac gagcagauca agaagauucu gaaccuuaaa     2040

uugaguggau gggguagacu gagcaggaag uuccucgaau ccaucacuuc ugagauaccu     2100

ggcuuuauug agccagucaa uaucaucacc gcuaugcggg aaacacagaa caaucugaug     2160

gaguuacugu cgagcaaauu caaguuuaac gaaaauauua aaaagaucaa cuccggguuc     2220

gaggauaccg acaagcgcuu uucauacaau agucuuguga aacccuuguu ccucucuccu     2280

agcguuaaga aaaugcugug gcagacucua aagcugguga aagagauuuc ccauauaacg     2340

caagcgccac ccaagaagau cuuuauugaa auggcaaaag gagccgagcu cgaaccggcu     2400

agaacaaaga ccaggcugaa aauccuucag gauuuguaua acucauguaa gaaugacgcc     2460

gaugcauuca gcucugagau ucaguaccug uuaggcaaaa ucgaaaacga ggacaaccuc     2520

cgucugcggu ccgauaaacu uuaucuguac uacacucagu uggguaagug cauguauugu     2580

gggaagccua uagagaucgg ccacgucuuc gacaccagua aguacgauau ugaccacauc     2640

uauccccaaa gcaaaauuaa ggacgauucu aucaauaacc gagugcucgu augcuccuca     2700

ugcaauaaaa acaaggaaga caaauaccca cugaagccug agauacagcu aaagcagaaa     2760

aaguuuugga auuuccugca gagaaacaau uuuauuagua ucgaaaaauu caacaggcuc     2820

acacgcacca cucccaucag cgaugacgag acagccaagu uuauugcucg gcaacugguu     2880

gaaacgagac aggccaccaa aguggcagcg aagguccuug agaagauauu cccagagacu     2940

aauaucgugu auucgaaagc ugaaacaguc uccauguuua ggaacaaguu cgauauuauc     3000

aaaugucgcg agauuaauga cuuucaucac gcccaugaug cauacuugaa caucgugguu     3060

ggaaaugugu auaacaccaa guucacaaau aaccccugga acuuuauaaa agaaaagcga     3120

gacaauccua agaucggcga uacuuacaac uacuauaaag uauucgacua cgaugucaag     3180

cggaauaaca uuaccgccug ggagaaagaa aagacgauca uuaccgugaa aacaaugcug     3240

aagcguaaua cuccaaucua uaccagacag gcugccugca agaaagggga guuauucaac     3300

caaacaauaa ugaagaaagg acucggucag cacccgcuga agaaagagug ucccuuuucu     3360

aauauuagca aguacggcgg guauaacaaa guuucagcag cuuacuauac uuucaucgaa     3420

uacgaggaaa agggaaauaa gaucaggagu cuugagacca uuccucugua ccuagagaaa     3480

gacauccaga agucccagga cggccucaaa gguuaucuga cggaucaauu ggggaagaaa     3540

gaaauaaaga uucuggugcc aaagaucaaa auuaacucuu uacucaagau caauggcuuu     3600

cccugccaua uaacaggaaa aaccggcgcc agcuucgucc ugcgcccugc ggugcaguuu     3660

uguugcucca acgacgaggu acuuuacuuc aagaaaauua uccgguuuaa ugaaaucaga     3720

ucacagaggg agaagauggg gaagacuauu agcccauaug aagaucugag uuuccgaucu     3780

uacaucaaag agaacuugug uaagaaaaca aagaaugacg agauuggaga aaaagaguuu     3840

uaugguuucc uccagaagaa gaaccuggaa auauacgaua ugcuacugau caaacacaag     3900

gacaccauuu auuccaaacg ccccaauagc acuacccuug auaugcucau caagggccgg     3960

uacgaguuua ucaaccugaa accggaagac caaauuagag ugauguugga gauacugaag     4020

uuauucugca caacgaggga gguuaucgau cucgggcuga uuaaaggaaa gucggaagcc     4080

ggcgucacca cucuugguaa gaaaaucuca aaccuggacu cuuguauuuu gaucuaccag     4140

aguauaacag ggaucuuuga gaagcguauu gaucuccuga aaaucuga                  4188


<210>  3272
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3272
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggu       60

ugggccguua cagacacuga uuauaaacuu cugaaggcua acagaaaaga cuuguggggc      120

augaggugcu uugaaacggc agagaccgcc gaagugcggc gcuuacaccg aggggcgcgu      180

agaaggauug agcggcgcaa gaagagaaua aaacugcuac aggaacucuu cucccaagag      240

aucgcuaaga cagaugaggg auuuuuccag aggaugaaag aaucucccuu uuacgccgag      300

gacaagacua uucugcagga aaaugcacuu uucaacgauc gggacuuuac cgauaaaaca      360

uaucauaagg ccuacccuac caucaaucac cugauuaagg cuuggaucga gaacaaagua      420

aagccagacc cgcgcuugcu cuaucuggca ugucauaaua uaaucaaaaa gcgaggccac      480

uuccuguuug agggugauuu cgacucagaa aaccaguuug auacaaguau ucaagcccuc      540

uucgaguacu uaagagaaga cauggaggug gacaucgaug cugacagcca gaaagucaag      600

gaaauucuga aagauuccuc ucuuaaaaau ucggagaagc agucaagguu gaacaaaaug      660

cuggggcuaa agcccagcga caaacagaag aaagccauca cuaaucucau aaguggaaac      720

aagauuaauu uugcggaucu guaugacaac ccugaucuua aggacgcaga gaaaaauucc      780

aucucuuuca gcaaggauga cuucgaugcc cuguccgacg acuuggcuuc aauucucggc      840

gauaguuuug aacuguuacu gaaagccaag gcaguguaca acugcagcgu ucucucuaaa      900

gugaucggcg acgagcaaua ccuuuccuuc gcuaagguca agaucuauga aaaacauaag      960

accgaucuga cgaaauugaa gaaugugauu aaaaagcacu uuccaaacga cuacaagaaa     1020

guauucgggu auaacaagaa ugagaaaaac aauaacaauu acucagccua uguuggagug     1080

uguaagacua aaagcaagaa gcuagucaua aacaauaccg ugaaccagga agauuuuuac     1140

aaguuccuga agcccauccu ccuggcgaaa agugagauua aggaggugaa ugacauccuu     1200

acagaaauag agacugguac cuuucugccu aaacagauuu cuaaguccaa cgccgaaauc     1260

ccauaucagu ugcggaaaau ggagcucgaa aagauccuga gcaaugcaga gaagcacuuc     1320

ucguuuuuaa aacaaaagga ugagaaaggc cugucucauu ccgaaaagau uaucaugcua     1380

cucacauuca aaauucccua cuacauaggg ccuaucaacg acaaucacaa gaagucauuu     1440

ccagaucguu gcugggucau uaaaaaggag aaaaguccga gcggaaagac cacgcccugg     1500

aacuucuuug accauaucga uaaagacaag acugcugaag ccuucaucac aucccgcacc     1560

aauuucugua cuuaucuggu uggcgagucu gugcuuccua agaacagcuu gcuguacuca     1620

gaguauacag uacucaacga aauuaauaac cugcagauaa ucauugaugg uaaaaauauc     1680

ugcgacauua aacuuaaaca gaagaucuac gaggaacugu uuaaaaagua uaaaaagaua     1740

acccaaaagc agaucaguac guucauuaaa cacgagggga ucuguaacaa gaccgacgaa     1800

gugauuaucu ugggaauaga uaaagagugc acauccagcc ucaagucuua cauugagcug     1860

aaaaauaucu uuggcaagca ggucgacgaa auuucaacua agaacauguu agaggaaauc     1920

auaagauggg cuaccaucua ugaugagggc gaagggaaaa caauucugaa gacuaaaauc     1980

aaggccgagu acggaaaaua cuguagugac gagcagauua agaagauccu aaaucucaaa     2040

uucagcgguu ggggcaggcu gucccggaag uuucuugaaa ccgugacauc ugagaugcca     2100

ggguucucgg aacccguuaa cauaauuacc gcaaugcgag agacucaaaa uaacuugaug     2160

gaacugcuca gcuccgaguu uaaauucacg gagaauauca agaaaaucaa cucaggauuu     2220

gaagaugugg agaagcaguu caguuaugac ucucugguca agccuuuauu ucugagccca     2280

uccgugaaaa agaugcuuug gcagacauug aaacucguaa aggaaauuuc acacaucacc     2340

caggcucccc cgaaaaagau uuucauagag auggccaagg gcgcggagcu ggauccuguu     2400

cgcacuaaaa ccagacuaaa gauccugcaa gaccucuaca auaacugcaa aaaugaugca     2460

gacgccuuua gcucugaaau uaaggaucug uccgguaaaa ucgagaacga agacaaccuu     2520

agguugcgga gugauaagcu guauuuauac uauacacagc ucgggaaaug uauguacugc     2580

ggcaagccca uugagaucgg acauguguuc gacaccagca auuaugacau agaucacauc     2640

uacccacagu cuaagauuaa agacgauucc aucucaaacc gcguccuggu gugcaguagc     2700

uguaauaaga acaaagaaga caaguacccu cuuaaagcug agauucaguc gaagcaaaga     2760

ggcuucugga auuuucugca gaggaacaau uucaucuccu uggagaagcu caaccgucug     2820

acucggacau cucccauaag cgaugacgaa acggccaaau uuauugcacg acagcuaguc     2880

gagaccagac aggccacuaa gguggcugcc aaaguucugg aaaagauguu cccagagaca     2940

aaaaucgugu auucaaaggc agaaaccgua aguauguuua ggaauaaguu cgauaucauu     3000

aaaugccgcg agauaaacga cuuucaucac gcgcaugaug cuuaccucaa uaucgucgug     3060

gggaacguuu auaauacaaa guucacuaac aaucccugga acuuuauuaa agagaagcgg     3120

gacaacccua aaaucgccga uaccuacaau uauuacaagg uguucgacua ugacgucaag     3180

agaaacaaua uuacggcaug ggaaaaagga aagaccauca uaacagugaa agauaugcug     3240

aagaggaaca cuccaaucua cacccgccaa gccgcuugua aaaaggguga gcuuuuuaau     3300

cagacaauua ugaaaaaggg cuuggggcag cacccgcuga agaaagaagg acccuuaucc     3360

aacaucucua aguacggcgg uuauaauaaa guaagcgccg cauacuauac ucucauugag     3420

uacgaagaga agggguccaa aauccgauca cuggagacca uaccucuuua ucuggugaag     3480

gacauucaaa agaacaaaga uggccuaaag agcuaccuca cggaccagau cggaaagaag     3540

gaaaucaaaa uucugauccc aaagauaaag auuaauaguu ugcugaaaau caacggcuuc     3600

cccugccaca uuacagggaa gaugggagag gcuuucguuu uacggccugc cguccaguuu     3660

uguugcucua augaugaagc gcucuauuuc aaaaagauca uacguuuuaa cgagauuaga     3720

aaacagaggg agaagauggg uaagaccauc uccccauacg aagaccugag cuuccgcucg     3780

uacaucaaag auaaucuuug uaagaaaacu aaggccgacg agauugaaga gaaagaauuu     3840

uaugaucugu ugcaaaagaa aaaccucgag aucuacgaca ugcugcuaac aaagcauaag     3900

gauaccauuu auaaaaagcg gcccaauuca gcuacuauag acauccuggu gaaaggcaag     3960

gagaaauuca agucucuuau uaucgaaaac caguuugagg ugauccucga aauucugaag     4020

uuguucagug caaccagaaa ugucuccgau cugcagcaca uagggggaag caaaaacgcc     4080

ggcaacgcua ggguugguaa uaagaucucu agcuuagaca acugcauucu caucuaccag     4140

uccauuacag ggaucuuuga gaaacgaaua gaccugcuua agaucuga                  4188


<210>  3273
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3273
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggu       60

ugggccguua cagacacuga uuauaaacuu cugaaggcua acagaaaaga cuuguggggc      120

augaggugcu uugaaacggc agagaccgcc gaagugcggc gcuuacaccg aggggcgcgu      180

agaaggauug agcggcgcaa gaagagaaua aaacugcuac aggaacucuu cucccaagag      240

aucgcuaaga uugaugaggg auuuuuccag aggaugaaag aaucucccuu uuacgccgag      300

gacaagacaa uccugcagga aaaugcacuu uucaacgaua aagacuuuac ugauaagacc      360

uaucauaagg ccuacccuac aauuaaucac cugaucaagg cuuggauaga gaacaaggua      420

aaaccagacc cgcgguugcu cuaucuggca ugucauaaua ucauuaagaa acgcggccac      480

uuccuguuug agggugauuu cgacucagaa aaccaguuug auaccaguau ccaagcccuc      540

uucgaguacu uacgagaaga cauggaggug gacauugaug cugacagcca gaaggucaaa      600

gaaauccuga aggauuccuc ucuuaaaaau ucggagaaac agucaagauu gaacaagaua      660

cuggggcuaa aacccagcga caagcagaaa aaggccauua cuaaucucau caguggaaac      720

aagauuaauu uugcggaucu guaugacaac ccugaucuua aagacgcaga gaaaaauucc      780

aucucuuuca gcaaagauga cuucgaugcc cuguccgacg acuuggcuuc aauccucggc      840

gauaguuuug aacuguuacu gaaggccaaa gcaguguaca acugcagcgu ucucucuaag      900

gugauuggcg acgagcaaua ccuuuccuuc gcuaagguca aaauauauga aaagcauaaa      960

acagaucuga ccaaguugaa agccgugauc aagaagcacu uuccaaauaa cuacaaaaag     1020

guauucgggu auaaccagga caaugaguca aagaacaauu acagcggaua uguuggugug     1080

uguaagacga aaaacaagaa acuggucauu aauaacagug ugaaucagga agauuuuuac     1140

aaguuccuga agcccauccu cucugcgaaa uccgagauaa aggaggugaa cgacauucug     1200

gccgaaaucg agacuggcaa uuuucuuccu aaacagauca gcaagucgaa cgcagaaauu     1260

ccauaucaac ugaggaaaau ggaguuggaa aagauccucu cuaaugcuga gaagcacuuc     1320

uccuuucuga aacagaagga ugagaaaggg uuaucacaua gugaaaagau uauaaugcug     1380

cuaaccuuca aaauccccua cuacauugga ccuaucaacg acaaucacaa aaagagcuuu     1440

ccagaucggu gcugggucgu uaaaaaggag aaauccccgu cuggcaagac aacucccugg     1500

aacuucuuug accauaucga uaaagaaaag accgccgagg aguucauuac aagccguacc     1560

aauuucugua cguaucucau aggugaauca gugcugccua agaacagucu uuuguacucc     1620

gaguauacug uacugaacga aaucaauaac cuccagauua ucauugacgg gaaaaauauc     1680

ugcgauacaa agcugaaaca aaagauauac gaggaccuuu uuaaaaagua uaaaaagauc     1740

acccagaagc agauuagcac uuucaucaaa cacgaaggaa uuuguaacaa gacagacgag     1800

gugaucauac ugggcauuga uaaagagugc accucuucau ugaagaguua caucgaacuc     1860

aaaaauauuu uuggcaagca ggucgacgag aucagcacga agaacaugcu ggaagagaua     1920

auccgcuggg cuaccauuua ugaugaaggg gagggaaaaa caaucuuaaa gacuaaaauu     1980

aaggccgagu acgguaaaua cuguuccgac gaacaaauca agaagauacu gaaucuaaaa     2040

uucucuggcu gggggagacu cucgaggaag uuucuggaga ccgugacaag cgaaaugcca     2100

ggauucuccg agcccguuaa cauuaucacu gcaaugcggg aaacccagaa uaaccuuaug     2160

gaguugcugu caagugaguu uaaauucaca gaaaauauca agaaaauuaa cucuggcuuu     2220

gaggaugccg aaaagcaguu cagcuaugac ggucucguga agccucuguu uuuaucccca     2280

ucagucaaaa agaugcugug gcagacccuu aaauugguga aggagaucag ccacauuacu     2340

caagcucccc cgaaaaagau auucaucgag auggcgaagg gggcagaacu cgagccugcc     2400

cgaacgaaaa cacgccugaa gauucuacag gaucuguacu cuuccugcaa aaaugacgcu     2460

gaugccuuua guagcgaaau ccaguaucuc ucuggcaaga uugagaacga agacaaucug     2520

agacuuaggu ccgauaaauu guaccuguau uacacccagu uaggaaagug uauguauugc     2580

ggcaaaccca ucgagauagg gcauguauuc gacacuucaa acuacgauau cgaccacauu     2640

uacccacaaa guaagaucaa ggacgauagc auuucgaacc ggguucucgu gugcuccucu     2700

uguaauaaaa acaaggagga caaauauccu cuaaagagcg aaauccaguc aaaacagcgc     2760

ggauucugga auuuucuuca gagaaacaau uucauaaguc uggagaaguu gaacaggcuc     2820

acccguacaa cccccauuuc cgaugacgaa acugcaaagu uuaucgcccg gcaacugguc     2880

gagacacgac aggcuacgaa aguggccgca aagguccuag aaaagauguu cccagagacc     2940

aagaucgugu acucuaaagc ggagacuguu agcauguuua gaaauaaguu cgauauuaua     3000

aagugcaggg aaaucaacga cuuucaucac gcucaugaug ccuaucugaa uauuguggua     3060

gguaacgucu acaauacaaa auucaccaac aaucccugga acuuuaucaa ggagaaacgc     3120

gacaauccua agauugcaga uacauauaac uacuauaaag uguucgacua cgauguuaag     3180

cggaacaaua ucacugccug ggaaaagggc aaaaccauaa ucacggugaa ggacaugcuc     3240

aaaagaaaca ccccaauuua cacaaggcag gcugccugua agaaagggga gcuguuuaau     3300

caaacuauca ugaagaaagg acuuggccag cacccguuga agaaggaagg ucccuucucc     3360

aacauuucaa aauauggggg cuacaauaag gucagcgcag cuuauuacac ccugaucgag     3420

uaugaggaaa aagugaacaa gauacgcagu uuagagacaa uuccucucua ccugguaaaa     3480

gacauccaga aggaucagga cgugcuuaag ucuuaucuga cugaucuacu cggcaaaaag     3540

gaaaucaaaa uucugguucc aaagaucaaa auaaauuccu ugcugaagau uaacggauuc     3600

cccugccaca ucaccgggaa gacgggagac agcuuugucu uacgaccugc cgugcaauuc     3660

uguugcucga augaugaggu gcucuacuuu aaaaagauua uccgguucaa cgagauacgu     3720

ucacagagag agaaaauggg uaagacaauu ucuccauacg aagaccugag uuuuaggucc     3780

uauaucaaag aaaaucuuug uaagaagacc aaagcggaug agaucggcga aaaggaguuc     3840

uacgaccugu ugcagaaaaa gaaccucgag auuuaugaua ugcugcuaac uaaacauaag     3900

gacacaaucu acaauaaacg ccccaacagc accacucugg ggaugcuuac caagggacgg     3960

uaugaauuua uuaaucucaa gccgaaagau cagauaaucg ucaugcugga gauuuugaag     4020

cuguucugca caacgagaga agccaucgac uuaggccuca ucaaagguaa gccugcugca     4080

gggguugcca cccugggcaa aaagauuucu aaccuugaua auuguauacu gaucuaccaa     4140

agcauuacug gaaucuuuga gaagaggauu gacuugcuca aaguguga                  4188


<210>  3274
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3274
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggu       60

ugggccguua cagacacuga uuauaaacuu cugaaggcua acagaaaaga cuuguggggc      120

augaggugcu uugaaacggc agagaccgcc gaagugcggc gcuuacaccg aggggcgcgu      180

agaaggauug agcggcgcaa gaagagaaua aaacugcuac aggaacucuu cucccaagag      240

aucgcuaaga uugaugaggg auuuuuccag aggaugaaag aaucuccccu guacgccgag      300

gacaagacaa uccuucagga aaauacucug uuuaacgaua aagacuucac cgauaagaca      360

uaucauaagg cauacccuac cauuaaucac uugaucaaag ccuggauaga gaacaaggua      420

aaaccagacc cgcggcuccu guaucuggcu ugucauaaua ucauuaagaa acgcggccac      480

uuucucuucg agggugauuu ugacucagaa aaccaguucg auacaaguau ccaagcauua      540

uuugaguacc ugcgagaaga cauggaggug gacauugaug ccgacagcca gaaggucaaa      600

gaaauccuua aggauuccuc uuugaaaaau ucggagaaac agucaagacu gaacaagaug      660

cuagggcuca aacccagcga caagcagaaa aaggcuauaa cuaaucugau uaguggaaac      720

aagaucaauu ucgccgaucu uuaugacaac ccugaucuga aagacgcgga gaaaaauucc      780

auuucuuuua gcaaagauga cuucgaugca uuguccgacg accucgccuc aauccugggc      840

gauaguuucg aauuacugcu caaggcuaaa gccguguaca acugcagcgu ucuuucuaag      900

gugaucggcg acgagcaaua ccuguccuuu gcaaagguca aaauuuauga aaagcauaaa      960

accgauuuga cgaagcuaaa gaaugugaua aagaagcacu ucccaaaaga cuacaagaaa     1020

guauuugggu auaacaagaa cgagaaaaau aacaauaacu acucaggaua uguuggugug     1080

uguaagacua aaagcaagaa gcugaucauu aauaacagug ucaaucagga agauuucuac     1140

aaauuucuca agaccauccu gucugcuaaa uccgagauaa aggaggugaa cgacauucuu     1200

acagaaaucg agacuggcac cuuccugccc aaacagauca gcaagucgaa ugccgaaauu     1260

ccuuaucagu ugaggaagau ggagcucgaa aagauccugu cuaacgcgga gaagcacuuu     1320

uccuucuuaa aacaaaagga ugagaaaggg cugucacaua gugaaaagau uauaaugcua     1380

cucacauuua agaucccaua cuacauugga cccaucaaug acaaccacaa aaagagcuuc     1440

ccugaucggu gcuggguggu caaaaaggag aaauccccau cuggcaagac cacgccgugg     1500

aacuuuuucg accauaucga uaaggaaaag acugccgagg aguuuauuac aagccguacc     1560

aauuucugua cuuaucugau aggugaauca guucuuccca agaauaguuu gcuguacucc     1620

gaguauacag ugcucaacga aaucaacaau cugcagauua ucauugacgg gaaaaacauc     1680

ugcgauacca aacuuaaaca gaagauauac gaggaacugu ucaaaaagua uaagaaaauc     1740

acgcaaaagc agauuagcac cuuuaucaaa cacgagggaa uuuguaauaa gacagacgag     1800

guaaucauau ugggcauuga caaagaaugc acuucuucac ucaagaguua caucgagcug     1860

aagaacauuu ucggcaaaca gguggaugaa aucagcacca agaauauguu agaggaaaua     1920

auccgcuggg caacaauuua ugacgagggg gagggaaaaa cuauccugaa gaccaaaauu     1980

aaggcugaau acgguaagua cuguuccgau gagcagauca aaaagauacu aaaccucaaa     2040

uuuucuggcu gggggagacu gucgaggaag uuccuugaaa cagucaccag cgagaugccu     2100

ggauuuuccg aaccagugaa uauuaucacu gccaugcggg agacgcaaaa caauuugaug     2160

gagcugcucu caagugaguu caaauuuaca gaaaacauca agaagauuaa uucuggcuuc     2220

gaagacguug agaaacaguu uagcuaugau ggucugguga agcccuuauu ccuguccccu     2280

ucagucaaaa agaugcuuug gcagaccuug aaacucguga aggagaucag ccacauuacu     2340

caggcuccac ccaagaaaau auuuaucgaa auggccaagg gggcagagcu ggacccggua     2400

cgaaccaaaa cacgccuaaa gauucugcaa gaucucuaca acaauugcaa aaacgacgcc     2460

gaugcuuucu cuuccgaaau caaggaccug aguggcaaaa uugagaauga agauaaccuu     2520

agauugagga gcgacaagcu guauuuauac uauacccagc ucggaaagug uauguacugc     2580

ggcaaaccua ucgagauagg gcauguuuuu gauacuucua acuaugacau cgaccacauu     2640

uacccccagu ccaagaucaa agaugacuca auuaguaauc gggugcuggu cugcagcucg     2700

uguaacaaga auaaagagga uaaguaccca cuuaaggcgg aaauccaguc caaacaacgc     2760

ggauucugga acuuccugca gagaaauaac uuuauaucuu uggagaaguu caauaggcuc     2820

acacgugcaa cgccuauuag cgacgaugaa accgccaaau uuaucgcucg gcagcuggug     2880

gagacucgac aggccacaaa ggucgcagcc agagugcuag agaaaauguu ccccgaaacc     2940

aagaucguuu auucaaaggc ugagacagug aguauguuua ggaacaaauu cgacauugua     3000

aagugccgcg aaauaaauga uuuucaucac gcccaugacg cauaccugaa caucgucgug     3060

gguaauguuu auaacacuaa auucaccaau aaccucugga auuuuauuaa ggagaaacgg     3120

gauaacccaa agaucgcgga cacguacaac uauuacaagg uguucgauua ugacgucaaa     3180

agaaauaaca uuaccgcuug ggaaaagggc aaaacaauca uaacugugaa ggacaugcug     3240

aaaaggaaua cccccaucua cacacgccaa gccgcaugua agaaaggggg acuuuuugau     3300

cagacuauua ugaagaaggg cuugggucag cacccucuga aaaaggaggg gccauucucc     3360

aacaucucua aauacggcgg auauaauaag guaagcgccg cuuacuauac cuuaauugaa     3420

uacgaggaga aaggcaacaa gauccgaucc cucgaaacga uaccgcugua ucuugugaag     3480

gacauucaaa aagaucagga cguucugaag ucauaccuaa cagaucuccu ggggaaaaag     3540

gaguucaaaa ucuugguccc caagaucaag auuaauagcc uguuaaaaau caacggauuu     3600

ccuugccaca uaaccgguaa gacuaaugac aguuuccucc ugcggccagc cgugcaguuu     3660

uguugcucua acgaugaagu gcuuuauuuc aaaaagauua uccguuuuuc cgagauuaga     3720

agccagaggg agaaaaucgg caagacaaua ucgcccuacg aagaccuguc auuccgcucu     3780

uacauuaagg agaauuugug uaaaaagacc aaaaacgaug aaaucgggga gaaggaauuu     3840

uaugaccucc ugcagaaaaa gaaucuagag aucuacgaua ugcugcuuac uaaacauaag     3900

gacaccauuu auaagaaacg gccuaacagu gcaacaaucg auauucucgu caagggaaaa     3960

gagaaguuca aaucccugau aaucgaaaau caauuugagg uuauuuugga aauccugaag     4020

uuauucagcg cuacgagaaa cgugucugac cuccagcaca ucggcgguag caaguacucc     4080

gggguggcca aaauuggcaa uaagauauca agucuggaca acugcauccu uauuuaucag     4140

agcaucaccg gaauuuuuga gaaaaggauc gaucuguuga agguauga                  4188


<210>  3275
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3275
auggacagcu ccaagcaggg ccugagaagg uaccaccucg ggcuugauau cggaaccaac       60

ucuauugguu ggacagccau agacgaucau uucucacugu ugcgggugaa aggcaagaau      120

gcuaucgggg uccgcacuuu uaaagaggga gaaacggcag ccgaccgacg uggcuucaga      180

accgcgaggc ggcgcuuaag uagaaggcgg uggcgccugc aauuucuaga ugaguucuuu      240

gcucccuauc ucgccgaagu ugacccuaac uuccuggcac gacuuaagca gagcgauauu      300

uccgccaaag acccagcuaa gaaucaggag uacaugggua agcuguuguu uccggaucag      360

gaagcagccu cugacgggaa cggauauccc acacucaucc aaaugagaaa agcuaugccu      420

gccgagaagg cggcagauuu cccaguguuu aauauuuacc agcugaggua ugcccugaug      480

aacgagaaac ggaaguucga ccuccgugaa aucuacuuag cuguacacca uauagugaaa      540

uaucgcggca auuuucugaa caauacuucg gucgauaagu ucaaggccga ccagaucgac      600

uuuaacaaau cauucgagac ccuuaaugau uuguucagac aggugcaacc cauugcaccu      660

uuucagaucg accugacaaa gguugaugaa cuaggcaaac uccugcuuga caccaagcag      720

aaaaagcugg auaggcagaa agcuguggcc aaguugcucc cacugauuga ggacgggacu      780

gauaaggaga ucgucaaagc gcaccaacag acagcccggg aaauaagcaa guuaauucug      840

ggauacaaag caaagguggg ucucauccuu cagaacaauc uggaccaaac cuuggauaug      900

aguacggaga acuccgacga ucagcuaaug cagauugcug aagaacugga cgacuaccag      960

auggaacuca ucaaucaacu gucucuucug uauagccaga ucauguugaa cgagauugua     1020

cccaacggcg agacuguuuc cgccucaaug cucaaucgau acuauaaaca ucgcugccag     1080

cugaaggaau uaaaggauua cggggcugcc caggacaaaa agaccagaga gcaacuggau     1140

caccuauaug cagaauacau aggacagauc ccuaaagaca guaaguucga uuuuacaaaa     1200

gaccucaaga agcugaugga uaaaagcgac cuuggccaga agauuaaaga ugagaucgaa     1260

gccggugacu auuugccaaa gcagaggacu ucugcuaacg gggugauacc gcaccaaaug     1320

caucagcagg agcuggauca gauuaucgag aaucaaaaag aauacuaccc cuggcucgcg     1380

gagccuaacc cagucgaaaa gaauaucaag aacgcaaaau auaagcugga ccagcuugug     1440

uccuuccggg ugcccuacua ugucggaccu augauuaccc uggccgauca gcaaaagaca     1500

ucaaaggcua gcuuugccug ggcaguucgc aaaaaggccg gcaaaaucac cccauggaau     1560

uucuacgaca ugauugacag agagaagagu gcuaacaauu uuauaaagag gaugacgaac     1620

aaagauacuu auuugcucuc ugaagacgug cugcccgccu ccagcuuacu guaccagaag     1680

uucacaguac uaaaugagcu caacaauauc cgugugaacg gccggaaacu gcagccggca     1740

cuuaagcagg cgauuuauac cgauuuguuu caaaaacgaa agacugucaa ucugaagaca     1800

cucaccaacu uccuggcuau ccacgagccu acguuaacca aacccaagau cgaagggcug     1860

ucgaacggaa gaucuuuuaa uuccucacuu gguacauaca uugacaugag uaaaauauug     1920

ggcgauagcc ucgacgaucu gaacuacagg gacgauaucg agaagauuau cgaauggucc     1980

acuguguucg aggacucuga gauuuauggg guuaaacuac aggaaaucca guggcugacc     2040

aaugagcagc gcgaaaagcu cgugcggaag agauaccaag gauggggcag gcugagcaaa     2100

aaguugcuua cagguauaac ugaugagaac gggcagcgca ucauugaccu gaugugggau     2160

accucaaaga auuuuaugca gaucguccau cagccagugu ucgaggagca aauugccgag     2220

uuaaaccagc agcuccugca agacgcagac aaggccccuc uugaaguagu ugaugacauc     2280

cuggcugaug ccuauacaag uccccagaau aaaaaggcaa uacgacaggu ggucaaggug     2340

gucgcugacg ugguuaaagc cgugggcaac gcgccagccu ccauuagcau cgaguucgcu     2400

cggaaugaag aucguaacaa ggaguugaaa aauucuagaa ggcacucaau ugaaaagcuc     2460

uaccacgaga ccgcagagaa acugguaaag cagacuaacc uacuggagga acucggaaau     2520

gucaaagacc ugaacgaucg cuauuaccuu uauuuuacgc aagccggccg ggacauguac     2580

gaugggacac ccaucaauau agacgaaauc aguacccauu acgagauuga ucacaucuug     2640

ccucagagcu uccugaagga caacuccuua gacaacagag ugcucguuaa gaaaucugaa     2700

aaugcugcca agucggauag ggugccagca aaacuguaug gagcgaagau gcgagccuuu     2760

uggaaacagc uuaaggagag ccagcugauu uccaaccgca aguaccuaaa ucucacuacc     2820

gacccggagc aagucgauaa auaucagaug caggguuucg ugcaucggca gcugguagaa     2880

acaagacaaa ucauaaaguu ggcugccaac auuuuuggcu cacuguaccc cgacagugca     2940

gugaucgaga ccagggcuga auuaacuaaa cagcuccgug agaaguucuc ucuguauaaa     3000

guucgcgaug ucaaugacua ccaccaugcc guggaugcau aucuuacagc cuuugcuggg     3060

cacuaccugu accagcggua uccuaaguug agaccaaugu ucguguacgg agacuaugcg     3120

aagaucuacg ccgaugaccu caaaaggcug cgaagcguca accuauuuca cgaucuggag     3180

aaggaccagu auaauuccgg cacggcaguu aaccccgaaa ccaaagagaa gauuguggau     3240

guggacuggc uuucagacua cguaagcaaa auccaacauu ucaaguauau gcucauuucu     3300

aaggcugucu acacucagca cggucagcug uacggggcca caauauugcc uguggcccaa     3360

guugauaaaa ucuccgcucc aauuggcauc aaggacgaua aacccgugga cauuuaugga     3420

gcauacacca augaagucgc cgcguauaug gcuaucgugc ggguuaacgg caagaaaccg     3480

aaguacaagg ugguaagugu cccugugcgc augcugcaga gauuaaaaau ggccgagcag     3540

aaggggcaug aucaguauau gcaaacacuc cacgacgugc ugcuuaggaa uuuuacuagc     3600

cugaaaaaga accggaaaac cggauugaag gaagcagucg uuaaagauuu cgacgugcuc     3660

guaccaugug ugcgauacaa ucagcugguc auagauggug acacgaaguu uacccuaggc     3720

ucugccacau aucagcacaa cgcuaagcag cuggugcuuu gcaaucauuc ccucgagacu     3780

cuggcaaaca acuucgagua cguuagaaaa caccccgaug aagccgacga gcgcaugauc     3840

auguuguacg augacauuuu agaccaagug aaucauuauu ucacccugua cgauaagaac     3900

aaauuuaggg aaaagcucaa ugcggggcgu gacaaauauc ugaaacuucc uacauucuca     3960

caguuugaga acaauaagaa aacugcuauc ggaaagagug aagccauuau caacauacug     4020

cggggcuugc acgcaaaugc cgcuauuacc aaccuaaaau gucugggugu cagcacgccc     4080

uucgggcagc uccagucgcc auccggcauc gagcugucug agaaugccaa acuuguguac     4140

caaagcccua caggauuauu uucaagagaa guucugcuca aagauuugug a              4191


<210>  3276
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3276
auggccaacc ugagaaauua caagggcgac uauaacaucg ggcucgauau gggaaccggu       60

agcgugggcu gggcugucac agacuccgag gggaaacuuc ugcacuucaa gaaacagccc      120

acuuggggau cuagguuguu ugauucagca gaaacggccg cgagugcucg gauuccucgc      180

ggccaacgac guagauacgu uaggcggcgc uggagauuag accugcuaca gcagcuauuc      240

caggaugagg ugagcaaggc cgacccagaa uuuuucauaa ggcugcggca aucccgccuu      300

cugaaagagg aucgagaaga gggucauaga gacuauaauc acccguuguu uaacgauucu      360

gacuucaccg agacagauua cuauaauagg uuucccacua ucuaccaucu ccggaagugg      420

cugauggaaa ccgacgagaa ggcagauauu cgucugguau aucucgccgc ucacaacauc      480

gugaaacauc gcgggaauuu cuuaagacag gaaaacacau cgcugucagc aaagaaugcc      540

aggccucuug acgcuuugcg ggaguuucga agcgcccugg ucgacuggug cgcggagcua      600

ggauacgaau guaacccaga ugagaguggc gaaauuucca augagauccu cucugugcug      660

gaagaggagc acguugcaaa cagcgacaaa aagaaacgca uauccucacu uaucggcgug      720

gccaugaaug aaggggcuaa ggagaguaag gccugcaacg augcacuggc uggagccaug      780

gucgguuuga aagcggaauu uaagaaugug uuuggcgaga uuagcgccga acagaccucu      840

aucuaccucu ccaacgacga ggcaguagau gcucugagag aggggauucc cgacgaagga      900

uuagagcugu ucggcaaacu cuguucaguu uauagcgccu acgugcuuca gggucuguug      960

aguuaugcuc cugggcaaac uaucucuguc aacauggugg ccaaguacga aaaauaugga     1020

aaugaucuaa agacacugaa agcacucgug ugcgaguacg ccagggcuga auaugacucc     1080

uuuuuccggg gcgaguucua ccgcgaucag gacggccacg agcugaacau auacaaugcg     1140

agcaaggcac agggguauac cagauacaac cuuggaacgu cgaagauguc uuaugaagau     1200

uuugagaaag ccgucaagaa acuguucgcu gguacugccg cagaagagga cggcagguac     1260

uugcagauga ugaaggaguu uggggaacaa cguuuccucc ggcgacugaa aaccuccgac     1320

aauggaucaa uuuauuacca guuacaucug gaggaacuaa cagccauccu caguaaccag     1380

ggcaagcacu auccauuucu gguugaggau gcugaaaaga uuaaaagccu uguguccuuc     1440

agaauccccu acuacguagg uccuuugacu gacaggaaug cccgcaagga uaaacauggg     1500

aagaaccggu uugcaugguc ugagagaaaa agcggcaugg aggacgcggu gaucacccca     1560

uggaauuggg augaagucau ugacagggga aagucagcug aggccuucau aacacgcaug     1620

accggcaugu guacguaucu gcaaggggaa gaugugcucc cgaagugcag ucugcuuuac     1680

gaggaauuuu guguucugaa cgaguugaau ggaucccgau gggguaugga cggcgauaaa     1740

gagcaccggu ucgacgcagc cgaucgugaa gcuaucauug aggaacucuu uaagaaaaag     1800

aaaacuguga aguauaagga cgucgccaac uggcuggaga gagaagcagg gaggaugaau     1860

gugcacguaa ugggaggcca gggugagaca ggguacgaga gcaaauuauc uucauauuac     1920

uucuuucgcc gggauaucuu cgacguugag gaacuggaca gagcugauua ucccaugaua     1980

gaacagauua uccuauggaa cacccucuuc gaggacaggc gaauccugaa ggagaaacuu     2040

gaagaugccu acggcccuaa gggaaauggc cgcuuggacg augaccagau uaaaaagauc     2100

ugccggaaaa gauuuacugg guggggaagg cugaguaaga aguuccucac agguauuaaa     2160

guggauaccg acuguggccc acguagcaua auggaugucc ugcgcgaggg ggaccccaac     2220

gcgggauuuc ggggcaagac gauggugaug auggaagucu uaagagauga gaaccugggu     2280

uuccaacaga aaguggacga auuuaauaag aacuacuucg ggcagaaagg caaggcccuu     2340

gagguuaaug aguugccugg auccccagcu aucaggcgau cucucaacca ggcaauucgg     2400

aucguggaug aaaucgccaa gauugcuggc aaagaccccg agucgauauu uaucgaagua     2460

acccgcgacg augagcugga caagagaggg aaaaggacaa agcggcgaua ugaugcccua     2520

aaagaagcac ugacugucuu caagaaggag gacccggaga uuugggcgga acuccaagcc     2580

accagcccuu ccgaucugga cgagagacuu acauuguacu uuaugcagcg cggaaaaugc     2640

cuguauucag gcgaacccau cgauauuaau aaguuaaguu cuggugagua cgaaguggac     2700

cauaucauac caaggagcua uaucaaagau gacucccucg agaacaaggc ucugguuugu     2760

cguaaauaca aucagucaaa gacugaugcc augcuucugg acccuagcau ucggagaaag     2820

augaggucuu auugggacau guugcucgau gcaaaacuga ucgggcccaa gaaacaccgc     2880

aaccuacugc gagaccauau ugaugagaag gcuaugaaag gauucguggc cagacagcuc     2940

gucgaaaccu cccaaagugu gaagcuggca ggcagccuuu ugucuguacg guacccaaau     3000

gccaacaucg ugcccguuaa ggcuuccaug ucacacgacc ugagggaggc ggccgguuuu     3060

gucaagugcc gcgaagcaaa ugauuuccau cacgcucacg acgccuacuu agccugccgu     3120

augggccucu uuauacagau gagacauccu aagguguaug agaacccaau ugcuaugaca     3180

cgggugaugc agaaauacgc acagcaacag aguaaggagu ucaauaggaa ccaccgcguc     3240

ccggggacca gcggauucau cguuaauucg uuuauguccu cuggcuucga uaaagaaacu     3300

ggggagauuu uuaaggacac guggaacgcc gaaacagaga ucgaaggaau acgaaaagcg     3360

cugaacuaua gacaguguua caucagccgg augcccaugg aggauaccgg cgcuuucugg     3420

aaugccacug uguauucacc uagggaccca aagauggguc ccaaacuugc acugccucuc     3480

aaaaaggggc ucgaucccaa aaaguacgga ggcuuuaguu cccaacaguu cgccuauuuc     3540

uucguguacg aggcucgcga caaaaaggau aagcagguau uuagauucgc agaaguccca     3600

guguggcugg ccucucagau ugagagcgac aaaaucgcgu uggaaaggua cgcuaccggu     3660

cuggccgagg cagaaaaguu aaaauuuguu cggauugagc gugccaagau ccucaaaaag     3720

caacugauag agauugacgg cgaucgcuuc auccuuacag ggaagaaaga aaugcgaaac     3780

ggaagagagc uggcuuuguc ccagaaggaa acccucaauc ugaucguggc cggcgacgag     3840

aacgcaucac uagaugacag ggggaaugcu agcgaugccg ucuucgacgc gauugugcgg     3900

cagggagaug aaaucggccu gcgccuuuau aaacagcuca agauugccga gauagcagag     3960

agauacagga guguuggugc ugacgccaaa gugggggcug uaaugucucu guuguccagc     4020

aucaacgcau cgucaaauau ggucaaccug ucuauggugg gaggcaguaa guaugccggu     4080

aauuuacaac cgaacuacuc caaucuccug agcgauccug aaacuaacuu ugugauuauc     4140

gaccagucug ucacaggcau guucgagcgg cgaacgagaa uugggcuuug a              4191


<210>  3277
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3277
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

auaacugaug acuauaaagu uccugcuaag aaaaugaagg ugcucgguaa uacgaacaga      120

gauuacauca aaaagaaucu ucugggcgca uuguuauucg acgggggaga gaccgccgaa      180

ggcaggcggc ugaagcgcac agcgcgacgu agauauacua ggcggaaaaa ccgccuaaga      240

uaccuccagg agauuuuugc ugaagagaug augcaaguag augaaucuuu cuuucagagg      300

cuggacgauu cauuccuugu ggaggaggac aagcagggua guaaauaucc aaucuuuggg      360

acccugaagg aagagaaaga auaccacaag aaguucaaaa caauuuauca uuugcgggag      420

gagcucgcca auagcaagga aaaagcagau cugcgccugg ucuaccucgc cuuagcucac      480

augaucaagu uucgaggaca uuuccuguac gagggcgacc uuaaagcaga aaacaccgau      540

gugcaggccu uguuuaagga cuucguugag gaauaugaua aaacuauaga ggagucccac      600

cugucugaaa ucacagugga cgcucuaucg auucucaccg agaaggucuc aaagagcagu      660

agacuggaaa aucuuaucgc ccauuacccg acggagaaaa agaacacucu guuuggcaau      720

uugauugcgc ucucccuggg guuacaagca aacuucaaaa ccaauuuuca gcugucugaa      780

gacgccaagc uccaguucag caaagauaca uaugaggagg accuugaagg acuguugggu      840

gaggugggcg augaauacgc ugaccuauuc guauccgcca agaaccugua ugaugcaauc      900

cuccugucag ggauacuuac uguugacgau aauaguacca aggcuccccu gagcgccucu      960

auggugaaaa gguacaagga gcacaaagaa gaguuggcgg ccuuuaagcg guucauuaaa     1020

gagaagcucc cuaagaaaua ugaagagauc uuuaaggacg auaccaaaaa cggauacgca     1080

ggcuacgucg gugcugacaa gaaacugcgu aagcgcuccg ggaaguuagc cacagaagag     1140

gaguucuaua aauacgugaa gggcauucug aauaaagugg aaggagcuga uuauuuucua     1200

gacaagaucg acagagagga uuuccucagg aaacagcgga cguuugacaa cggcucaauc     1260

ccacaccaaa uucaucugca ggaaaugcac gccauacuuc gacgccaggg ggagcauuac     1320

cccuucuuga aggaaaacca ggauaaaauc gagaagauuc ugacuuuuag aaucccuuau     1380

uacgucggac cacucgcaag gaaggguagc cgguucgccu gggcugagua uaaagcggac     1440

gaaaagauaa cacccuggaa uuuugaugac auucuggaua aagagaagag ugcagaaaaa     1500

uucaucaccc gcaugacucu uaacgaccug uacuugccug aggaaaaggu ucucccaaag     1560

cacucuccgc uguacgagac auuuaccgug uauaaugagu uaacgaaagu aaaguacgug     1620

aacgaacaag gcgaggccaa auucuucgau accaauauga agcaggaaau cuuugaccac     1680

gucuucaaag agaacagaaa ggugacaaag gauaaacugc uaaauuaucu caacaaggaa     1740

uuugaggagu ucaggauugu uaaucugacu gggcuugaca aagaaaacaa ggcuuuuaau     1800

gccuccuugg gaaccuacca ugaucugcgu aaaauccucg acaagagcuu ccuggacgau     1860

aaggugaacg agaaaauuau agaagacauc auucagacau uaacucuguu ugaggaucgg     1920

gagaugaucc gacaaagacu ucagaaguau ucggacaucu ucaccacaca gcaguugaaa     1980

gaacucgaga ggcgccacua caccggcugg ggucggcugu cuuauaagcu aauuaauggg     2040

auaagaaaca aagaaaauaa gaaaacuauc cuggauuacc ucauugacga uggcuacgca     2100

aacaggaacu uuaugcaacu gaucaaugac gaugcccuuu ccuucaagga ggaaauugcu     2160

cgcgcccaga ucauaggaga cgucgaugac aucgcaaacg ugguacauga uuugcccggc     2220

agcccugcga uuaagaaagg gauccugcag aguguuaaga uuguggacga guuagucaaa     2280

gugaugggac acaauccaac gaacaucaua auugagaugg cucgagaaaa ucagacaacc     2340

gacaaggguc ggcguaacag ccaacagaga cucaaacugc uucaggauuc ccugaagaau     2400

uuggacaacc ccgucaauau caagaacgug gagaaucagc aacuccagaa cgauaggcug     2460

uuucuauauu acauucagaa uggcaaagac auguauacug gggaaacccu ggauaucaac     2520

aaucucucuc aguacgacau agaucauauc auuccucaag ccuucaucaa ggacaacagc     2580

cuggauaacc gcguucuuac acggucagac aaaaauagag gaaagaguga ugacgugcca     2640

uccauugagg uaguccacga aaugaaauca uuuuggucua aguugcuguc agugaaguua     2700

aucacccaga ggaaauucga caaccucacu aaggcagagc gaggcggucu gacagagaau     2760

gauaaagccg gguucauaaa gcgccagcuu guugaaacgc ggcaaauuac caaacacgug     2820

gcucaggucc uggacgccag auuuaacgca aagcaugaug agaauaagaa agugaucagg     2880

gacguaaaga ucauuacucu aaaaaguaac cucgugagcc aguuccguaa ggauuuuaaa     2940

uucuauaagg uucgcgaaau caaugacuac caccaugcuc acgaugccua ucugaacgcg     3000

gucauuggca cagccuugcu gaagaaauac cccaaguuag cuuccgaguu uguguacgga     3060

gaguucaaaa aguaugacgu gcggaaauuu auagcaaagu cugauaaaga aaucggcaag     3120

gccaccgcua aguacuucuu uuauucgaau cucaugaacu ucuuuaaaaa ggaggucaaa     3180

uucgccgacg ggacaguugu ggaaagaccg gauauugaga cuagcgaaga cggagagauc     3240

gcauggaaua agcagaccga uuucaaaauu gugaggaagg uacuguccua cccucaaguc     3300

aacaucguga agaaaacgga gguucagacc cacggucuug accgaggcaa gccccggggg     3360

cuguuuaaug cgaacccauc accuaaaccc aagccagaca guucugaaaa uuugguggga     3420

auaaaacgca accucgaucc caagaaguau ggcgguuacg ccgggaucag caacuccuau     3480

gcuguccugg ugaaagccau uaucgagaag ggcguuaaaa agaaagaaac aauggugcua     3540

gaguuccagg gaauuucaau ccuggacaga auaacuuuug aaaaggauaa gagggcauuc     3600

cuucucggca aaggguacaa ggacauuaaa aagaucaucg agcugccuaa auacagcuug     3660

uuugagcuga aggauggauc ucggcgaaug uuagcuucca uucucaguac caauaacaaa     3720

agaggcgaaa uacauaaggg uaaugagcug uucguaccac agaaguuuac aacucuucug     3780

uaucacgcca aacgcaucaa caauccgauu aacaaggacc auaucgaaua cgucaaaaag     3840

cacagggaug acuucaaaga guugcucaau uaugugcugg aauuuaacga gaaguacgug     3900

ggggcaacca agaauggaga gcgucuaaaa gaagccgucg cugauuucga cagcaagucu     3960

aacgaggaaa uuugcacguc cuuucugggc gcgguuaaua guaaaaacgc cggucuuuuc     4020

gagcucacau cacugggcag cgcaucggau uuugaguucu ugggggugaa gaucccccgg     4080

uauagagacu acaccccuuc cucuuuacug aaagauagca uacucaucca ucaaucaauu     4140

acuggacugu augaaacaag gaucgaccuu aguaagcugg gcgaggacug a              4191


<210>  3278
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3278
augaagagag ugaacgagga cuacauccug ggccucgaua uugggaccaa uagcugcgga       60

ugggccguca cagacaaaaa gaacaaucuu cugaaauuga gggguaagac ugcuauaggc      120

ucccacuuau ucgaagaggg gcauacggca gccgaucggc gcggauuucg aaccacacgu      180

agaaggcuga aacggcgcaa guggagacua aggcuccugg aagagaucuu cgcggaaccc      240

auggcuaagg uugacccugg cuuuuucgug cggcuucacc agucuugggu aucaccacug      300

gauaaagacc gcaagaaaua uaacgccauu guguuuccga cugcaaagga ggaucaagcc      360

uucuacaaac auuaugcuac caucuaccac uugcgagacg agcucaugac acaggauaga      420

caguuugacc ugagggaaau uuuccuggca auccaucaca uagucaagua ucgggguaau      480

uuucuccagg auacccccgu gaaggacuuc gaggccagua aaaucgaagu ugggccuauu      540

uuaagccaca ucaacaaugc uuuugccgag aagauugugg aggaucaaga cccaaucgaa      600

cugaacgucg cgaaugcagc cgacauagag gaugugauuc guggaaaaga cgcugaaaag      660

acuguauaca aacuugauaa gguuaaaaag aucgccaagu ugcugacaga cuccaccgca      720

aaagaggaaa agaacguggc uaaacagauu gccaaugcga ucaugggcua uaagacgcag      780

uucgagacua uccuagauaa agagauugac aagucugaua aggcccagug ggaauuuaaa      840

cucucggacg cagaugcuga cgauaagcug gacgcccuuc ugcccgacuu ggaugagacc      900

gaccaaacag ucguggcuga aauagagaaa cucuucucag ccaucacucu gagcaccauu      960

guggaugaaa acaagaguuu aucccagucu auggucgaga aauacaagaa gcauaaaaag     1020

gacuacaaaa agcugaaaaa guauaucaau acacuccagg aucagaccaa ggcaaagaaa     1080

cuucuguugg ccuacgaccu auauguuaac aaucgccacg gcagacugcu cgaggcuaaa     1140

aagacguucg aagauaaaaa gaaaaagaag gcgcugacua aagacgaguu uuacaagaua     1200

gugaaagaua accuugacga uagcgaccug gcacaugaaa uucaacagga gaucgccgcu     1260

gauaacuuca ugccuaagca gaggacaaau uccaacgggg uaaucccauu ucaauugcac     1320

cagauugaac ucgacaaaau cauugccaau cagggaaagu auuaccccuu ccuggcagcc     1380

gagaacccug uggaggacca ccggaagcag gcuccauaua aauuagauga acugguccga     1440

uuucgcgugc cguacuacgu uggucccaug auaaccgccg acgagcaaga aaagacuuca     1500

ggcaaaaguu ucgcauggau ggugagaaag gaggaugggc agaucacacc uuggaauuuu     1560

gagcagaaag ucgacaggca ggaaagcgcg aacaaguuca uuaagcggau gaccaucaaa     1620

gauacguauc uacucucuga ggacgugcug ccagcuaauu cccuuuugua ccaacgcuuu     1680

gaaguacuga acgagcucaa uaacaucaga auuaauggau caaggauaag cguugaucug     1740

aagcagcaga ucuucaacga ccuuuuugaa gagaaaaaga ccgugacaga gaaaagucug     1800

acuucuuauu ugaagcagaa ucuccaucug cccaccgucg aaauuaaggg cuuagccgau     1860

ccuacaaaau ucaacuccag ccuggcaucg uacuaucacc uaaagucucu ccauguguuu     1920

gacaaagagc uggccgaucc acaauaccag aaggacuucg aaaagaucau ugaguauucc     1980

ucaaucuucg aagauaagaa aauauuucag gacaaacuuc acgcugaguu caagugguug     2040

acucccgagc aguuuaaagc caucaguacc uggcgucugc aagguugggg gcggcucagc     2100

cgaaagcugu uagucgaacu gcaugacaca aauggacaga acauuaugga gcagcuuugg     2160

gauucccaga aaaacuucau gcagaucgug accgaaccgg acuuuaagga ugcaauugcu     2220

aaagagaauc agaacguuac uagagccaau ggcguggaag agaucuuggc ggacgccuac     2280

acgucuccug cuaacaagaa ggcaauaagg cagguaguca aagugguugc cgaugugguc     2340

aaggcugccg gcgggaaaaa gcccgcacaa uucgcgauug aguuuacacg cgacccagau     2400

aaaaauccuc agcucagcca cauccgggga accaagcugc uaaaggccua ccaggaaacu     2460

gcuggugagc ugguggacca gaaacucacc gauucacuga aggaagccau gacaaguaga     2520

aaacuuuuga aggacaaaua uuuccuguac uuuaugcaag caggcaggga ugcuuauacc     2580

gggcagaaga uuaacaucga cgagguaucc acuaauuacc agauagacca caucuuaccc     2640

cagagcuuca uuaaggauga cucuuuugau aaccgcgugc ucacagccac gccacugaau     2700

gcagagaaau cagacgaugu ucccuauaag cgauucgcca acaauuacgu cagugacaug     2760

aaaaugaccg ugggagaaau guggaagcau uggcaaaaag cuggcaucau uaacaagcac     2820

aaacuuggua aucuguugcu cgauccugac cggcugaaca aauuucaaaa gagcggguuc     2880

aucaaucguc agcuagugga gacuucccaa auaauuaaac uggucucugu uauccuccag     2940

aacaaguauc cagaugcgga aaucauuaca gugaaagccg gcgacaacuc ggcacugaga     3000

cagaggcuua auuuguacaa gagccgcgau gugaacgacu accaucacgc uaucgaugcc     3060

uaucugucca uuauaugugg aaauuucuua uaccagguau auccgaaaua ccggcccuau     3120

uuugucuacg gcaaguauaa gaaauucuca caagacccug accuccagaa ggaggugauc     3180

aaacacuuua agggguucac cuuuaugugg ccacugcuuc agaaagauaa cagugaaaga     3240

aaggcccccg agaagauuaa agagaauaac ucugacagga ucguuuucua caagcauccu     3300

gauauuuuug acaaacugcg aaaggcuuac aauuauaaau acaugcuagu gagccgcgaa     3360

acaacuaccg agaacuccgg acucuucgau gucacgaucu auccacgggg ugaaagagac     3420

cuggcaaaga ccaggaaguu gauacccaaa ucaaauggcc uggauccgaa gaucuacggg     3480

ggauauagcg gcaacacaga cgccuacaug gugauuguua aaaucgauaa ggguaaagag     3540

ucuauuuaua aggugaucgg gguaccuaug cgugcguuag cuucccucaa ucgcgccaag     3600

aaacagggca acuacaagga ggaacugcac caaguccuug agccacagau aauguuugac     3660

aaaaauggaa agcccaaacg gagugugaag ggcuucagaa uuaucaaaga ucaugugccu     3720

uuuaagcagg ucguucugga cggggauaag aaauucaugu ugaacagcuc uacuuacgaa     3780

aucaaugcaa agcagcucac ccugacaccc gagacuauga ggauugugac cgacaaccua     3840

aagaagggag aagaccaaga ucagcugcuu guaaaagccu augacgagau acuccagaag     3900

guggaucagu accugccauu guucgacguc aacaaguuuc gaaauucccu gcacuuaggu     3960

cgggcuaaau uccucgaucu ggcagugaac gacaagaaaa ucacgcuuac aaauauucug     4020

aacggcuugc augauaaucu cguuaccccu gaccugaaga acaucgggau uaaaacuccc     4080

cuaggaaagc ugcaagugcc aucaggcauc guccuuagua gcgaagccau acucaucuuu     4140

cagucgccga caggucuguu cgagaagcgc gugagaauug cggauuugug a              4191


<210>  3279
<211>  4194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3279
augaaggacu acaacaucgg ccuggauauu gggaccagcu ccauaggauu cgccgcuauc       60

gacgaugaca auaaccucau uagagugaaa gguaagaaug ucaucggcuc uaggcuuuuu      120

gcagagggga aaacagccgc ggaacggcgc ggauuccgaa cugcucguag aagguauaac      180

cggcgcaagu ggagacugaa auuguuagau gagauuuucu acgaagagau cauacacauc      240

gaccccgagu ucuuuaauag gcugaagcag ucaaguauua gcccuaagga ugaguccaaa      300

aaguauuucg gcucucuacu cuuuccagac aaaacggauu cggccuacua ucaugacggu      360

aaccaaacca ucuaccaccu ucggaaucgc cuuguucauc agaaggagaa agcagauauu      420

cgagaaaucu aucuggccuu gagacacaua gugaaguaca gggggaacuu ccucgacuca      480

acaccgguaa gcaguuuuga ggcugauaau cugaagcugg acgaacucgu gcccgucauu      540

aacgacaugu acgagaauau cgauauugac uucuccauca acguggauaa uaucucugag      600

auugaaaaag uuuuacugga caaccaucuu aagagcgcag auaaacagga ccgguugaag      660

gagcugcuau augauaaucc ugaauaccca gacgauaaag ugaagcagaa agagauaucc      720

aaguuucgua aggaccucga uaaagaaauc auuaagguca ucgugggaua uaaauucaac      780

gccuacaaga uaguaaacua uauucccgac gacggcucaa aauuuaguuu cagcuucucu      840

gaugaguccu cagacgauaa gcuggacgau cuuagcgagg uucuggacga uagucaaaug      900

gaaaucuuga auacucucaa ggugaucuac ucucgcguca gacugaacca gauuaucccu      960

aauggcaugg gguuauccga gagcauggug gcuaaauaua acaggcacca cgaacagcug     1020

gaggaccuca aggaacuucu ggauguguug ccaaaugacg agaaacgguc gauugaguac     1080

gccuacaccg cguauauagg aaacgucgau aagcagaaau acaauaacaa ucuaguugca     1140

ucucugaagg accuccccau caagggugau auuaacaaaa agauccaagc cguguuugaa     1200

uaugcuaaag gccauaucuc cucacaggac cuguacgacg uaauuaagac aauaaucauu     1260

gauaaauauc uugaggacga aaguuucgag uaccacgauc ugguggaacg aaucagcaau     1320

gacauugaua acaaggacua uuugcucaag cagcgcuccu cucagaaugg gaacaucccu     1380

caucaagucc accagaugga gauggauaaa auaaucgcca aucagagcca auacuaccca     1440

uuucugggag uguuaaaccc gaacgaguca agaaggcaca uugcaaagua uaaaaucagu     1500

gaacugguuu ccuuccgggu gcccuacuau gucggcccuc uaauuacccc agaggaacag     1560

gagaagacua gcgguaaaca uuuugcuugg augaagcgca aagaggccgg gaagaucaca     1620

cccuggaauu ucgaagagaa gguggacaaa gaagcgaccg ccgcaaaguu uauaaaaaga     1680

cucacgguaa aggauacuua ccugcuuaac gaggacguuu ugccugauaa uucucugcuc     1740

uaucagcagu ucaaagugcu gaacgaacuu aauauuguca aggugaacaa uaagaggcug     1800

accuugucag agaaacaagg agucuacaac gaccucuuua agaauacaaa aaguaucacu     1860

gugaagcguc ugaaaaacua uuuaauuagc aauuacaagc accuggagaa caucgaaaua     1920

accggccuau ccgauggcga caaguucaau aacucucucg ggacauacaa ugacuuuaaa     1980

aagaucuucg gagcucggau ugaugacguu aacuauucgg ccgauuuuga gaacaucauu     2040

gaauggagca ccguguucga ggaccgaucc auccugaaag aaaagcugga gaaagauaua     2100

gacuggauua cgccagagga aaucaaggcu guagucgcca agagauacuc agguuggggc     2160

agguugagug caaaacugcu cacugagcug uuagaugacg augggcagcg caucauugac     2220

auccugugga augauucuau uacauucaug gaagccguga gcaagcccga gauaaaagcu     2280

cagauccaga acauuaaucg ggacuuuauc aaggcgaccg acauugaaac uguucuugau     2340

gaggcauaua cauccccgca aaacaaaaag gccaucagag agguguacaa auuggucaag     2400

gacauacaga aggcuauggg aggcaaagcc ccuucaagca ucucuauuga auuuaccagg     2460

ucccccgagg auaaugguag ugugacgaag acccgcuaua acaauaugca gaacaaauac     2520

aaggaacucg ggaaagagcu gagcgacgau cuaucugcac agcugaagga guauaagggc     2580

cgaauggacc aagauaaauu uuaccucuac uucaugcaga ugggaaagga ccuguaugau     2640

ggcuccacaa ucaaucuuga cgauuugaac ucauaccaga uugaccauau cguaccacag     2700

aguuuuuaua aagaugacag cauagacaau cgggugcuga cucacuacac caacaaucaa     2760

aagaaaggga accauacacc uuuaaaugga cucaacauug augcccagac ucagaaccug     2820

uggaagcacc uuaauaagcu ggguuugauc ucgaaaaaga aacuccaaaa ccugugcuuc     2880

aaggacauca aucagcuauc caaauauacc aagcacggcu uuguucguag acagcugguc     2940

gaaacaucuc aggugauuaa gcucguggcu aacauacuga gcgaugaguu cgccaaugac     3000

gggaccaaaa ucauugaagu caaggcaaaa augaacucac uuaugaggga uacuuuuaau     3060

uuguacaagg uucgccaacu gaacgacuau caucacgcgg uggaugcuua cuuaacgaca     3120

uucgugggaa aauaccucua uaagcgguac cccaagcuga gaaguauguu uguauauggc     3180

gacuucaaaa aguuuuccgc cgaggaugac cuuaaagaua ucaagaccuu caauuuccug     3240

caugacauua cuaacccaaa agaaucuaau aaggagaaaa ucuacgauga aaagagcggu     3300

gaacuaauac ucgacaggaa gcacgcaauc gagaccauua aaaagaucua ucaguacaaa     3360

uuuaugcuga uuguccgaga aguguccaca aagucagggc aguuguucaa acagaccauc     3420

uauagcgcca agaagacuaa cggccugaaa agugcuauac ccguuaaguc uaauuuagag     3480

ccuuccauuu acggauaccg cagcggcaac guggaccacc auauggucau cgugaaaauc     3540

aagaaaggga caaaguauuc guuuaagguu auuggaaucu cagcccgggc aauacaagaa     3600

cucgagauug cuaaaaagau cgguaauuac agagauaaac uguaugaggu gguaucugac     3660

aggcuuaguc aguccaugaa aagcgucgaa ucuuuccgua uuauccugga uaacauaaug     3720

uacggccagu ugauugugga cgggaaugau aaguuuacgc ucggaagcgc cgacuaucgc     3780

aagaacuccc ggcagcuggu gcuauguacc aacucacuga agauccuuaa agaugaggac     3840

uacaugaaua gacucaguga uauccaaagg agcaagcuga ugguccaggu uuaugacgaa     3900

auuaucaaaa uuaugaagaa caaauacucu uuguucauau ccaauacuga gaagcuguua     3960

gaaaaacgag auaucuuuug cggccucuca uucgacgagc agaagauugu gaucuggaag     4020

aucauuauag accugcacaa cgguccauuu acaaaaaucc aagagcuugg gauuaauaac     4080

aagaugggca ccuuccagac aaaacaugga aucauucuga cugaaaaugc gaucguaaua     4140

uaccagaguc cgagcggcuu guuugagcgg aaggugcgcc ucaaagaucu guga           4194


<210>  3280
<211>  4194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3280
augaagaaag ugcugggccu cgaccuuggg accaacagca ucggaugggc ccuggucaau       60

gaggcuacaa aggaagagaa aaaggaaggu aaauccucug agauuauaaa guugggcguu      120

agagugaacc ccuuaacugu agaugaaaag acgaauuucg agaaagggag gccucuguca      180

accaacgcag accggacacu aaagcgcagu gcccgacgua aucuccagag auacaaacug      240

aggcggcgca accuuaucga gauucugauc aagaauggau uuauuacuaa agauacccca      300

uugacagaaa ccggcaagga cacuacacac caaacccuca agcugagagc gaaaagcgcu      360

aaggagaaag uggaucugaa ggacuucgcc aaaauccucu uaacgauaaa caagaaaagg      420

gguuauaagu ccucucggaa ggucaauucg gaugaagacg ggcuggcaau cgauggaaug      480

gccguggcua aacaucuuua cgagcagaac uugacuccgg gccaguaugu ucugaaucua      540

cuccagaaga acaaaaagua cgugcccgac uuuuaucgcu cagaucugca agaggaauuu      600

aaaaauauuu ggaacaauca gaagaccuac uauuuugaca uccuuacaga uacucuguuc      660

gaggaauuga aggacaaaaa caagagccag accugggcaa uuugcaaaaa gccuuuugac      720

aucgccggca uaaaacagau ugggaaggcu agugauaagc gacucgagag auacaaaugg      780

aggacagaag gacugaccca aaaguuaaac cuggaguacc ucgccaucgc gcuucaggag      840

auuaauaacg acaucaauaa auccucuggu uaucugggcg caaucagcga ucgguccaag      900

gaauuguucu uuaacaaaga gacggucggg gaaaaucuau accgucagcu ggugaagacu      960

ccacacacau cacuccgcaa ccagguauuc uauagacaag acuaccugga ugaguuugaa     1020

accauuuggg agacucaggc caaguucuau ccccagcuua cacauacccu gaaagaggaa     1080

auaagggacg uuaucauuuu cuaccaacgg aaguugaaaa gucaaaagag ccucaucucu     1140

uucugucagu ucgaguccug ggaacagacg uauauagaga aagaaaccgg aaagucaaaa     1200

acacgaacug ugggccgcaa ggucauuccu aagagcaguc cacuguuuca ggaguucaaa     1260

aucuggcaga auuuaaacuc ucuggaguuu agaaauuccg augaagguga gaucauugag     1320

uucaucaagc uagacgaaga uauuaggcag accguguuug aggaacucaa ccugcggggg     1380

aaucuuaaac ccaacgacgu guugaagaua cugggaaauu acaucagccu cggcaaaguc     1440

ucgcagugga agugcaacuu cgaggaaauu gagggcaauc gcacaaacgc ugcccuguac     1500

aacguuuauc aaaucaucgc agaaaaugag ggguacggau uugauugggc uaagaaaacu     1560

gccucugaga uuaacaagga acuuaaaaau auauucccuc agcuggguau cgacgcgucc     1620

auuuuggacu uugauuucac ccaggccaac uuugacaagc agacaucaua ucaacucugg     1680

caccuguuau acagugcaga ggaagauuuc aaaaucagcg aggaagacaa ggugcuguau     1740

ggcaauuccu cuguaagccu aaagaaaaag cuccaucaga aauacggguu uaagccagag     1800

uauaccaaac ugcuuacuaa cauuucauug cagccggauu acggaaaucu gagugcuaag     1860

gccaucugua acauacuccc cuaccugaag gagaauaacg uguauuccga ggcuugcgac     1920

uuaacgggcu acaaucacag caacuuccug acagccgaag aacaggcaaa aagaccucuu     1980

aagccaaucu uggagcuccu gaaaaagaac ucucuaagga aucccgucgu ggagaaaauu     2040

cugaaucaaa ugguuaacgu ggucaaccag gugaucgaaa ccuaugguaa gccugaugag     2100

auucguaucg aacucacucg ggagcugaaa aagucagcca aggaacgagc uaauaugacc     2160

aaaaguauag cggacgcaac aagaaggaac gaugacuacc gcaagaccau uaucaaagag     2220

uucgggauuc caaaucccac uaagagcgau guaguucggu auagacuuug gcaggaguug     2280

gccuccaggg gcuacaaaga caucuuuaca gauacggaaa uaaagccgca agagcuguuc     2340

ucuaacaaga ucgacauuga acacaucauu ccuaaggcuu uacucuuuga cgauucguuc     2400

agcaauaaga cccuggccuu uaaaaaggac aaccuuaaaa aggcaaaucg cacugccuau     2460

gauuucaucg cuaacgacua cgcccccaau cuggagaacu acauagaacg aguggagucc     2520

uugcauaagg aucggaaaau uacacgugca aagagaaaua aacuccugau gcuacaguca     2580

aagcugccag acggauuuau cgagagggau cuccgcaaca gucaguauau caccaaagaa     2640

gcgaagaaga ugcuguacga ggucugucgg acaguggucu cuacuagcgg cuccauuacc     2700

gacaaacuua gagaagauug ggacuugauc aaugugauga aggagcugaa cuucccuaaa     2760

uauaaggcuu uagggcucac ggagaaaauu gaaagguacg auauaggagc cgagaagaug     2820

aaggaaguug agaucauuga agacuggaau aaacgaaacg aucaccgcca ucacgcaauc     2880

gacgcccuga ccguggcuuu uucaacacac agccauauuc aguaucuuaa ccaccugaau     2940

gcccggagag acgaggguca uaagaaacac gcagcuauca uagccaucga aucuacuauu     3000

accgagaagg agggcaacaa aaagaggcgu uucaucgcgc cccuaccaaa uuuucgccuc     3060

caagccaaag aacauauuga guccauccug guaaguuuca agaacaagaa uaaagucgug     3120

acaaacaaua uaaacaagac uaaacggauu aagaaagaua aguuuaauac caagguucag     3180

uugacgccca gagggcagcu gcacaaagaa acaaucuacg gaaagaucaa acagccuuua     3240

gagaagcaca uuaaacucaa caagagcuuc acccuggagc aagcuaaacu uauaaucgac     3300

aaaaggcagc gagaaaaggu gcugaaucau uuggagaaau augcacacaa cucugauauu     3360

gccuucaauu ccaagacucu caaaaagaac ccacugcuaa ucucagacga accgcugaag     3420

gagguccuuu gcuuugaaga gguguucaca auucggaaag auaucaguua cgacaauuuu     3480

aagaacgaga aacagcucga uaagguaaua gacgaaaaag ugaagcaggc ucugaaagag     3540

cgcaucaauc aauacaacgg cgauuucaga gaagccuuua gcgacauuga gaggaacccc     3600

aucugguuga auaaggcaaa ggguauuugu guuaaacggg cgaccaucaa gggggucucg     3660

aacgccgaac cucugcauac ugcuaaaaau cacuuaggcg aggaggugcu caccgaaaac     3720

aaggagcaga uaccagugga uuaugucucc acaggaaaua accaccaugu ugccauuuac     3780

gaagacgagg acggcaaucu gcaggaaaaa guggugucuu ucuaugaggc aguaauccga     3840

gucaacgagg ggcuucccau cauugauaag gaauacaaua agagccuggg auggacguuu     3900

aaauucacca ugaagcaaaa cgagauguuu guguucccuu cagaagacuu uaauccaacu     3960

gagaucgauu ugcucgacca gaaaaacgcu agucugauau cccccaaucu auucagaguu     4020

cagaaguuca caauuaaaga uuacuuuuuc cgccaccauc uggagaccac aguggaaaac     4080

aauaacacuc uuaagaacac cacguggagg cgugaggguc ucucuggcau cuaugggauu     4140

aucaagauac ggcugaauca cuugggagaa auuguccagg ugggcgagua uuga           4194


<210>  3281
<211>  4194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3281
augaacgaga auaaggaaug guuccugggc cucgacaucg ggaccgccag cgugggaugg       60

gcugcaacag auacuaacua caaucuucug aaauugaagg guaaaaaguc cuggggcguc      120

agauuauuug acgaggccca gacggcgaaa gaaaggcggg auugccgcuc uaagcgacgu      180

agacuggcua ggcggaagug gcgccuaggg cuccuggagg aacuuuucgc cgaggagauu      240

aacaaaauag accccaacuu uuucaugaga cugagggaau caaaguauaa aguuagugau      300

aagaccggaa augugccuag caucuuguuu aaugagaaaa acuacacaga caaggaauau      360

uaccggaauu auaagacuau uuaccaccuc cgcgcagauc ugauccauaa aaacaaucca      420

gacauucgag agcuguuccu cgccauccac cauauauuaa aguauagagg ccacuuucug      480

uucgcugguc aacaguuuaa aggguccgag aucaaggaua acauuaggga acuuuugcag      540

uacaauucuc ugaacaucaa aauugacauc accgaugagc uaauaaauaa cauugcagaa      600

auccuccuga agaaaaagaa cucgacagac aagauuaucg aguucaaaaa gcuguuugcc      660

gaugaaaaac agcuuaucga ggcuuugcgg cucgcccugg gaaauaagac ccaauuagcg      720

aaacuguucg acauugaggc auacaaggaa cucgacauau caaucaagac uauuagcuuu      780

aaagagaaga ucuaugaaga uguacguagu aacuacgagg aaauacuugg cgaguauauu      840

aucgugcugg acacagccaa auugaucuac gaugcuauua uccuauccaa gauuaaaaua      900

aagaauauga ccaucucuga gagcaagguc guguccuaug aaaaacaugg caaggaccug      960

aaacagcuca agcaccugau uaaagaggau uguaaacuuu caaagaaaga aaagcgccag     1020

cuguucgaga acaucuucgu uaaagaagac gagaaggggg ccaauuacgu gaacuauauc     1080

ggaaagaaua acgcagguag uagcaagucu uccugcucau acgaggauuu uaaaaaguuc     1140

uugacgaagg aacucaaaug uugcgaggaa agcaagcuga aacaggacau uuuagcugag     1200

cuggagcuag aaacuuuucu cccgagacaa aggaccaagg auaauagugu cauacuguac     1260

cagauccaca aagaggaacu ugagaagauu uugaacaaug ccgugcagua ucaucccuuc     1320

cugaacaaua cagacgaaua cggcaucucu auuaaggaga aaaucauaca gcuccuggag     1380

uuucggaucc cuuauuacgu agggccacuu aacacuuccc acaagguugg agauggcggu     1440

uucagcuggu cggugcgaaa uaaaggguau gaacauauug cggucacccc cuggaacuuu     1500

aacaagguga ucaauaaaga ggaaucugcc aagaacuuca uugagaaucu gacaaaccaa     1560

uguaccuacu ugcucuauga agacgugcug ccuaagcacu ccuuacugua cucagaguuu     1620

gcacuacuca augagcugaa caguaucaau uacggaggca aacgccuuac ggucgaacac     1680

agaaacuuga uaauugagag guucuuuaag cagagcgcuc caaaagccac uggcaaggaa     1740

cugaaaaagu uccucauguc cgaggggguu aucgacggaa aggaugaacu gucugguuuu     1800

gacgugagca uuaaaaauga uuuaaaguca uaucgggacu ucaaaaagau ccugggcgau     1860

aaauucgaca gugagaaggu agagaaaaug auacgcugga ucacacuuuu uggggaaucc     1920

aagcaguugc ucguggauaa gauuaccaaa gaguacggag acaucuauag ccuggaagag     1980

auuaagaaaa ucucuggccu aacuuacaag gauuggggua gacugucaga gaaacuccug     2040

acagaaauag ucaguagcca acuuuaugac gaguccaccg gggaaugcuu caacauuauc     2100

aaugcuauga ggaacaauac guugcuguuu auggaguuac ucggcaccaa cuacgauuau     2160

uucgaaaaga ucaauauuua caaccgucgg cugcgaaagg agaucacaga gauuacuccc     2220

cauauacuug acgaucugua cgugucuccu gccguuaaaa gaucgaucug gcaagugguc     2280

aggauugugg aagaguugaa gaaaaucauu ggaagcgaac caaagcgcau cuuuguagag     2340

accacacggu ccaacaaaga aaagaagggc gacgacggga gaaaagauuc aaggaagaaa     2400

gcacuccugg agcuauauaa gaaaauaaag acuuccgaca ucguugagcu ggaaaaggcc     2460

cucggacaca ccguggauuu cgacaaaaag aaacaggauc ugacagacau ugauaauaac     2520

aaacuuaagg cucgcaaguu guaccuguau uacacccagu uaggucgaug ucucuauacu     2580

ggcaaggcga ucgaccugaa uaaccuucug ucuagcaauu ccgaguacga ugucgaccau     2640

auuuauccgc agucaaaaac gaaggauggg agcuggaaca aucuagugcu cgucaacaaa     2700

acagaaaauu cuaagaaauc caacggacug aucagugaga gcauacagcg gaagaauaaa     2760

cacuuuuggc guuucuugaa ggcaaagaac cugauuucug aagagaaaua caagagauua     2820

accaggacug aggacuuuac cgaugacgaa cucuccggcu ucaucgcccg ccagcugauc     2880

gagacaucac agagugugaa agcuguuagc caaauucuuc agaaucugaa ccccaccacu     2940

gaaauaugcu uuucgaaggc cgagaaugug caugacuucc ggcaggaguu ugguaaaauc     3000

aagguaggga agagaaggaa cuugacagaa gaacagcaaa aagaagcaaa ggagggccag     3060

cucauuaaag uccgagaucu gaacgaccua caccacgcca aggaugcuua ccugaauauc     3120

gugguuggaa acguguauca uauuaaauuc acgcgcaauc cuaagaacuu cauccggaag     3180

aauaguaaaa acagagaaua cacccuuaau acucucuuuu auucugacaa gauagagagg     3240

aacggcgagg ucgccuggga aaaagggaag acagagaaaa ucgugagcuc aguaaugugu     3300

aauaaccuga uuaaugugac ccgucgcaca agugaacaga agggagcauu guacgaugcg     3360

acuaucuaua aggcuaacgc cgcaaaagag gguuccuacu auccaguuaa gaccaaaaau     3420

aagaaccuga aagacgucac gcgguacggc ggguucaccu cuauuaagau cgccuacuau     3480

agcauauuua aauacgauau uagaaauaag gacaagguga aagaaaacau caagaucauu     3540

cccaucccua uauauauuuc ccagaauauc aaaacagauu uagaccucau uaacuucgcu     3600

gagaagcaaa ucaaacugaa gaagggagag aacauagaaa aucuucagcu gauuuacaaa     3660

aaguugaaca ucggcgcccu cgugaaaauc gauuauuacc cauauuacgu cggugggaag     3720

acuaaugaca acuuuuacau ugacggcgca guucaggugc ugcuaucaag cgauaucgag     3780

agguaucuga aagaaguggu aaaguucguc aauuuucaac gagagaacaa ggaagcuaaa     3840

gugaguaagg agcacauuuc uuccgaggaa aauauagagc uuuacaacac ccuccugugc     3900

aaaaugacag ccaagaucuu cacucauacc aaaaauaaca aguauaauga cuuggcgcag     3960

gauaaggcca aaaacacguu uaagaauuua agccuggagg agcaguguaa aguucuccug     4020

gagauucuua accuguugac aaauaagaaa accacuuucg accuaucgcu gaucaacgaa     4080

aaggcuucac gggugaaaug cggauuuaac cuccugaauc agaagcaauu cucuaucauu     4140

gagcagagug ucacaggccu uuacgaaaag gagauaaaaa ucauugaaga uuga           4194


<210>  3282
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3282
auggagaccc aggccagcaa ccaacugauc uccucucacc ucaagggcua ccccauuaaa       60

gacuauuucg uggggcuuga uauaggaaca ucaagugucg guugggcugu uacuaauaag      120

gcauacgaac uguugaaauu uagaagccau aagauguggg gcuccagguu auucgacgag      180

ggggaaucug ccguggcgcg gcgcggauuu cgaucgaugc guagaaggcu ggagcggcgc      240

aaacuaagac ucaagcugcu ugaagaacug uucgcugaug ccauggcaca gguagacccu      300

acguuuuuca ugagguugcg ggagucaaag uaucacuacg aagauaaaac cacaggccau      360

agcaguaagc acauccucuu uauugacaaa aacuauaaug aucaggacua cuucaaggag      420

uauccaacug uguaccaucu gcgcuccgaa cugaugaaau cugguaccga ugacauccga      480

aagcucuuuu uagccgucca ccacauucug aaguacagag ggaacuuccu uuaugaggga      540

gcuacauuug auagcaaugc auccaccuug gacgauguga ucaaacaggc ccuggagaac      600

auaacuuuca auugcuuuga cuguaacuca gcuaucagua gcauuggcca aauccuaaug      660

gaagccggca agacaaaauc ugacaaggcg aaagcaauug agcaucucgu ugauaccuac      720

aucgccacgg acacugugga uaccuccuca aagacacaga aagaccaggu caaggaagau      780

aagaaaaggc ugaaggcuuu cgccaaucuu gugcuggggu ugaacgcaag ccucauagac      840

cuguucggaa guguagagga auuagaggag gaucugaaaa agcuccagau uacuggugac      900

accuaugaug acaaacggga ugaacuugcu aaggccuggu cugacgagau cuacauuauc      960

gacgauugca aguccguuua ugacgcgauc auucuguuga gcauaaaaga accgggccua     1020

acaaucucgg agucuaaggu gaaagccuuu aauaagcaca aagaugaccu ggcaauucuc     1080

aagucccugc uuaagucaga ucguaguauc uacaacacca uguucaaagu cgacgaaaag     1140

gggcugcaua auuaugugca cuacauaaaa caaggacgca cggaggagac uagcuguaac     1200

agagaagauu uuuacaagua uaccaaaaag auuguggagg gcuuguccga cucuaaggau     1260

aaagaauaca uccucagcca gaucgagcug cagauuuuac ugccccuaca gaggaucaag     1320

gacaauggug ucauuccuua ucaacuccac cuggaggagc uuaaagcuau auuggccaag     1380

ugcgggccaa aauuccccuu ucugaacgag guugcugaug gauucucagu ggccgaaaag     1440

cucaucaaaa ugcuggaguu ucggauuccu uacuauguag gcccacuuaa cacacaucac     1500

aauguggaca acggcggguu cgcaugggcc guccgaaagg cuaguggacg cgugacuccc     1560

uggaauuuug augacaagau cgacagagaa aaauccgcgg cagccuucau caagaaccug     1620

acaaauaaau guaccuacuu gcucggugag gauguucugc cuaagagcuc uuuacuguau     1680

ucagaguuua ugcuacucaa cgaacugaau aacgugagga uugacggcaa accacuugag     1740

aaggucguga aggaacauuu gauagaggcu guauucaaac aggaucacaa gaaaaugacg     1800

aagaaucgga ucgaacaguu ucugaaagac aacggguaca uuagugagac ccauaagcac     1860

gagaucacag gacucgaugg cgaaauuaag aaugaccugg ccagcuaccg cgauaugguu     1920

agaaucuuag gugacggguu cgauaggucc auggcagagg aaauaaucac ugacauuacc     1980

aucuucggcg agucuaaaaa gaugcugcgu gaaacacuuc ggaaaaaguu ugccucgugc     2040

uuggaugacg aggcuauuaa aaagcucacu aagcugcgau auagagauug gggaaggcua     2100

agccaaaaac ugcucaacgg caucgagggg ugugacaagg ccggagacgg uaccccggaa     2160

acaauaauua uccugaugcg caauuucucc uacaaccuua uggaguugcu gggcgauaaa     2220

uuuucauuca uggaacggau ucaggagauc aaugcaaagu uaaccgaagg gcagauagug     2280

aacccccacg acaucauuga ugaccucgcg cugaguccug cugucaaaag agccgugugg     2340

caggcacuua ggaucgucga ugagguggcc cauauuaaga aagcucugcc agcccgcauc     2400

uuuguugagg ugacucgauc uaacaagaau gaaaagaaaa agaaagacag ccggcaaaag     2460

cguuuguccg aucucuaugc agcuauuaaa aaggacgaug uacugcuaaa cggacugaau     2520

aacgagauau ucggcgaacu caagucaagc cuggccaaau acgacgaugc ggcccuuaga     2580

ucuaagaaau uguaucugua cuauacgcag auggguaggu gcgcuuacac aggggagauc     2640

aucgaguuau cccuccugaa uaccgacaac uacgacauug aucacaucua uccccgcagu     2700

cuuacuaagg acgauagcuu ugacaaucug guccuaugua aacggaccgc aaacgcccag     2760

aagucugaug cuuacccuau uuccgaagag auacagaaga cacaaaaacc auucuggacc     2820

uuucucaagc agcagggccu gaucucagaa agaaaauaug agagguugac ucgaauuaca     2880

ccccugacgg ccgacgauuu aaguggauuc aucgcacgcc agcucgugga aaccaaucaa     2940

agcguuaagg cggccacuac acugcuucgg agacuguacc cgggcgugga cgucguguuu     3000

guaaaagcug agaacgugac cgauuucagg caugacaaua acuuuauuaa gguucguucg     3060

uugaaucacc accaucacgc caaggaugca uaucucaaca ucgucguggg gaauguguac     3120

caugagcgcu ucacacggaa cuucagagcc uuuuucaaaa agaacggagc uaauaggacu     3180

uauaaccugg caaaaauguu uaauuacgac gucaacugca ccaaugccaa ggaugguaag     3240

gcuugggacg uuaaaacguc cauggacacc gugaagaaaa ugauggauuc uaacgacgug     3300

cgaguaacaa agcggcuacu ggaacagacu ggcgcgcuug ccgaugcaac cauauacaaa     3360

gcuacagucg ccgggaaggc caaagacgga gcuuauaucg gcaugaagac uaaaagcuca     3420

guguucgcag auguuaguaa guacgguggg augaccaaga uuaaaaaugc cuauuccauc     3480

auugugcagu acacgggcaa gaaaggagag gucaucaagg aaauagugcc ucuccccauu     3540

uaucugacaa accgcaauac cacugaccag gauuugauca acuacguugc gucuaucauu     3600

ccacaagcua aagacauaag caucauuuau ggcaagcugu guaucaauca guuagugaag     3660

guaaacgggu uuuacuacua ucucggaggc aaaacaaauu ccaaguucug cauugauaac     3720

gccauccagg ucauaguguc aaaugagugg aucccuuacc ugaaagugcu ugaaaaguuu     3780

aacaacauga gaaaagacaa uaaggaucug aaggcaaacg ucguuagcac cagggccuug     3840

gacaauaaac acacuauuga ggugcggauc guagaggaaa agaacauuga guucuuugau     3900

uaucucguga guaaacugaa gaugcccauc uaccagaaaa ugaaggguaa uaaggcugca     3960

gaacuaucug agaaagggua uggacuguuc aagaaaaugu cccuugagga acaaagcaua     4020

caucucauug agcuguugaa cuuacugacc aaucagaaga caacguuuga agucaaacca     4080

cucggcauca ccgccucgcg aucaacugug gguucuaaga ucaguaacca ggacgaguuc     4140

aaaguuauua augaauccau cacaggccug uacagcaacg aggugaccau agucuga        4197


<210>  3283
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3283
auggaaaaga gcuacuccau cggccuggac auugggacca acucuguggg auggucaguc       60

auaacagaug acuauaaagu ucccgccaag aaaaugagag ugcucgguaa uacugauaag      120

aaauacauca agaagaaccu ucugggcgcu uuguuauucg acagugggga aacggcagag      180

gccaccaggc ugaaacggac agcgcgccga cguuauacua gaaggaagaa ucggcuacgc      240

uaccuccagg aaauuuuugc uaaagagaug gccaagguag augagagcuu cuuucaaaga      300

cuggacgagu ccuuccuuac cgaugacgau aaaacauuug acucucaccc uaucuucgga      360

aacaaggcag aggaagaugc cuaucaucag aaguuuccaa ccauuuacca ccugaggaaa      420

cauuuggcug acucgcagga gaaggcagau cuccggcuga ucuaucuggc ccucgcucac      480

augauaaaau uccgcggcca cuuuuuauac gacaauuuca acgacgauaa uuuugacugg      540

cgaaacaucg auauucagaa gagauacgaa gaguucaucg agacuuauga cucaacacug      600

ggugaaagcu accuugccga uauuagugug gacgcggcau ccaucuugga ggaaaaaguc      660

ucuaagaccg agaggcugga aaaucuacuc aaauauuacc cgacggagaa gaagacuacc      720

uuuuucggga accugauaaa acuuauucug ggacaacagg ccaaguucaa agcuaucuuu      780

aauuuggagg augaaauuag ccuccaguuc gccacaccca cuuaugacga ggaucuggaa      840

gaguuacugg gcaagaucga caacggcgau uccuacucag aacucuuugu ggcagcucag      900

aaucuuuaua acaccauccu guuggccagu uuccuaaaaa cagacaauaa gagcgcgaag      960

gccccucugu cuaccuccau gauugagcgg uacgagaacc auaaaaagga ccucgcaaaa     1020

cugaaggauu uuguuaaaaa gaacugccca gaccaauacc acgauauauu ccgugacaag     1080

ucaaagaaug gguaugcugg auacaucgau aacgguguga gccagaauga cuuuuauacg     1140

uucauuggca agugucuuga agagagucug aaaaaggaua aaggggccca guacuuuuug     1200

gacaagaucg aucgcgacga uuuccucaga aagcagagga cuuuugacaa cggagcuuuc     1260

cccuaucaaa uacaucugca ggaaaugcac gccauuuuac ggcgacaggg cgauuacuau     1320

ccuuuucuga aagagaauca agacaagauc gagaaaaucc uaacauuccg cauuccauac     1380

uacgucgguc cccucgcaag aaaggacucu agguucgccu gggcuaacua ucgguccgau     1440

gaagcgauca ccccuuggaa uuuugacgag gugguagaua aagaaaagag cgcagagaaa     1500

uucauuacuc gcaugacacu gaacgaccuu uacuugccag aagagaaggu ucugccgaag     1560

cauucgcuca uauaugagac cuuuacggug uacaaugaac ugaccaacau caaauauguc     1620

aaugaucagg ggaacgccau ucacuucgac ucugagcuua aggagaaaau cuuuaaucag     1680

cuguucaagg aaaacagaaa aguguccaag aagacauuga ucgauuuucu caauaacuca     1740

gaaggaauuu acacugacaa acuggugggc auagaugagg aggucaagua uuuaaacgcu     1800

agucugggca ccuaccacga ccucaaaaag auccuagaga gcuucaugga ugacgaaauu     1860

aaugagaaaa ucauugaaga uaucauacag acacugacuc uuuuugaaga caucgagaug     1920

aagaggcaac guuugcagaa guacgacgau auuuucaccc ccaaacagcu gaaggagcuc     1980

gcccggcgaa acuauacagg guggggaaga cuguccuaca aauuaaucaa ugguauuagg     2040

aacaaggaaa auaacaaaac cauccuggac uaucuuaaga auggcaaccg caauuuuaug     2100

caguugauaa acgaugaccg gcucucuuuc aagcaaauua ucauugaugc aagaaaaauc     2160

gagaagcugg acaauauaga aagcguugug uacaaccuac cugggucacc agccaucaaa     2220

aagggcauuc ugcagaguau caaaauugua gaugagcucg ugaaggucau gggacauaau     2280

cccgacaaca ucgugauaga aauggcuagg gagaaucaga cuacgaacca ggguaaaaac     2340

cgcucccaac agcgacugaa gcggcuucag gauagcaugu cuaauuuuaa ggacucaagu     2400

auuagcuuga aagauguuga caacuccgac cugcagaaug aucguuuauu ccucuauuac     2460

auccaaaacg ggaaggacau guauacaggc gaggaacugg auaucgacca ccuuucugau     2520

uacgacauug aucauaucau uccucagucg uucauaaaag acaauagcau cgauaacaga     2580

gugcugaccu ccucagccaa gaauagggga aaaagugacg augucccagg ccgcgacgug     2640

guauugaaga ugaagcccuu uuggaaaaag cucuacgaug uuaaacugau uucuaagcgg     2700

aaauucgaca accuaacuaa gagcgagcac gggggacuga ccgaauccga caaggcaggu     2760

uuuaucaaaa gacagcucgu ggagacaagg caaauuacca aguaugucgc gcagauccug     2820

gauggccgau ucaauacuaa acgcgacgau aacaauaagg ugauacggga cgucaaagug     2880

aucacacuua agagcucauu gguuucucag uuuagaaagg auuucggguu uuacaaagug     2940

agggagauua acgacuauca ucacgcucac gaugccuacc ugaaugcagu agucggaacg     3000

gccaucuuaa agaaauaucc gaagcucgcu ccugaguucg uguacggcga auauaaaaag     3060

ugcgacguuc guaagcugau ugccaaaucc ggugauaaga gugaaaucgg gaaagcaacc     3120

gcuaaguacu uuuucuacag caaccuuaug aacuuuuuca aacgcgugau acgguauucu     3180

aauggcauga uugucgugag acccguaauc gaguacucca aggacacugg agaaaucgcc     3240

ugggauaaag agaaggacuu caagacagug uguaaaguuc ugucaugccc acaggucaac     3300

auugugaaga aaguggagaa gcaaagucau ggccuagaua gggggaaacc uaagggauuu     3360

uauaaugcga accccagccc aaagcccaaa aaggguucga aagucaaucu cguuccuaua     3420

aaggccaacc ugaauccgaa aaacuacggc ggguaugcug gaaucuccaa uucuuacgca     3480

guguuggugg acgccaccau ugaaaagggc gcuaagaaaa agcugacacg aauccaggag     3540

uuccagggua uuagcaucau agaccgggaa aaauaugaga agaacaaagu agaauuuuua     3600

aaggggcucg gcuacaagga gaucuacuca auuaucacuc ugccaaaaua uagucuuuuc     3660

gagcuggccg auggaucccg cagaauguug gcaucuauuc ucagcaccaa uaacaagagg     3720

ggcgaaaucc acaaagggaa ugagcugguc cuacccgcga aguacauacc ucugcuuuau     3780

caugccaacc ggauucacaa uacguuugaa accggacacc gagaguacgu ggaaaaacau     3840

aucgcugagu ucaaggagau cgccgaaauu auccucgagu uuaacaauaa auauguuaac     3900

gcaaagaaaa auuccucaau aauugaaaaa gcucuggaga gcuucgacag uuuuucuuug     3960

gaugagaucu gugacuccuu cgugggcaag cugaaaaaga acaacacuaa aaagaauagc     4020

gguuuauuug aacucgucuc gcugggguca gccucugauu ucgaguuucu ugaaacaaag     4080

gugccaagau accgcgacua uacccccagu ucccuguuga acgcaacacu cauucaccag     4140

agcaucacug gacuguacga gaccaggaua gaucuaucua aacugggcga agaguga        4197


<210>  3284
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3284
auggagaccc aggccagcaa ccaacugauc uccucucacc ucaagggcua ccccauuaaa       60

gacuauuucg uggggcuuga uauaggaaca ucaagugucg guugggcugu uacuaauaag      120

gcauacgaac uguugaaauu uagaagccau aagauguggg gcuccagguu auucgacgag      180

ggggaaucug ccguggcgcg gcgcggauuu cgaucgaugc guagaaggcu ggagcggcgc      240

aaacuaagac ucaagcugcu ugaagaacug uucgcugaug ccauggcaca gguagacccu      300

acguuuuuca ugagguugcg ggagucaaag uaucacuacg aagauaaaac cacaggccau      360

agcaguaagc acauccucuu uauugacaaa aacuauacug aucaggacua cuauaaggcc      420

uacccaacca ucuaccaucu gcgcgcugag cugaugaaag augguacaua ugacauucga      480

aaacucuucu uagcagugca ccacauccug aaguacagag ggaauuuucu uuaugaagga      540

gccuccuucg auucuaacgc uagcaccuug gacgaugucc ugaaacaggc ccuagagaau      600

auaacuuuua acugcuucga cuccucagcg gcaaucggca guauuggcca aauccucaug      660

gagacaggga agaccaaaag cgacaaggcc aaaacgauug aaaggcuggu ggauacuuac      720

aucauaaccg acgagguuaa ugcuucugga aagacacaga aagaucaggu gaaggaagac      780

aagaaacggc uuaaggccuu ugcaaaccug gucuuggguc ucaaugcuuc ccugauugau      840

uuauucggcu caguggagga acuggaggag gaccucaaaa agcugcagau cacuggggau      900

accuaugacg auaaacguga cgaacuugcc aaggcgugga gcgaugagau uuacaucauc      960

gacgacugua agaguguaua ugaugccauu auauugcuau cuaucaaaga accgggacug     1020

acaauuuccg agagcaaggu uaaagcauuc aacaagcaua aagacgaucu cgcuauccug     1080

aagucgcuuc ugaagucuga ccgcuccaua uacaaugcca uguuuaaagu ggaugaaaag     1140

ggcuugcaca acuacgucca uuauauuaaa caagguagaa ccgaggagac gucaugcaau     1200

agggaagacu ucuacaagua uacuaaaaag gugguggagg ggcuccccga uaguaaggac     1260

aaagaauaca uccugagcga gaucgaauua cagacacugc uaccucucca gcggauuaag     1320

gauaacggag ucaucccaua ucagcugcac cuugaggagu ugaaaguuau ucuggcuaag     1380

uguggcccca aauuuccuuu ccucaaugaa guggccgacg gcuuuuccgu agcagagaag     1440

cugauaaaaa ugcuugaguu ccgaauccca uacuaugugg ggccccugaa caccucucac     1500

aacgucgaua auggagguuu ugccugggcu gugcgcaagg cgagcggcag aguuacuccu     1560

uggaacuucg acgauaagau ugacagggag aaaucagcag ccgcuuuuau caagaauuug     1620

acaaacaaau gcaccuaccu ccugggggag gacguguuac caaagaguuc ccugcuauac     1680

agcgaguuca ugcuccugaa ugaacuuaac aauguccgga ucgauggaaa accguuggaa     1740

acgaagguga aggcccaucu gauuaaagca guauuuaagc aagaccacaa aaagaugacc     1800

aaaaaccgca uagagcaguu ccucaaggau aauaacuaua uccccgaaaa gcauaaaccu     1860

gagauuacag gccuggacgg ugagaucaag aaugauuuag ccucuuacag agacaugguu     1920

aggauucugg ggaacaauuu ugaucuuuca auggcugaag agaucauaac ugacaucacc     1980

auuuucggcg aaaguaaaaa gauguugcgu gagacacucc ggaaaaaguu cgccagcugu     2040

cuggaugacg aagcaaucaa gaaacuaucc aagcugcgau auagagauug gggaaggcuc     2100

ucuaaaaagc ugcuuaacgg cauugacggg ugcgauaaag cgggagacgg uacuucggug     2160

accaucauag aggcuaugcg cgacuuuagc uacaauuuga uggagcuguu aggcgaucgg     2220

uuccucuuua uggaaagaau acaggagauc aacgacaagc ugacagaagg gcaaauuguc     2280

aacccacacg auaucauaga cgaucuugcc cugucccccg cagugaaaag ggccgucugg     2340

caggcuuugc gcaucgugga cgagauuguu cauaucaaga aggcccuccc ugcacgaauu     2400

uucguggaag uaacccgguc aaauaaagcu gagaagaaaa agaaagauag ucgucagaag     2460

agacugucug accuauaugc cgcgaucaag aaagaugacg cccugcucag cggacugacu     2520

aacgaggaau uuggcggucu uaaguccgag uuggcuaaau acgaugacgc agcccugagg     2580

ucaaagaaau uauaucucua cuacacgcag auggggcgcu gugcuuauac aggcgaaguc     2640

auagaccuga gccaacuuaa uaccgauaac uacgacauug aucacaucua uccacggucu     2700

cugacuaagg acgauuccuu cgacaaucua gugcucugca agagaaccgc caacgcacag     2760

aaaagugaua cauaccccau cagcaaggag auucagaaag cgcagaaggc cuuuuggauc     2820

uuccugaaac aacagggauu gauuucugag aggaaguaug aacgacugac ccgcauaacu     2880

ccguuaacag cugacgaucu cuccggcuuu aucgcccggc agcugguuga gacgaaucag     2940

ucagugaagg cagcuaccac ucuucugaga agguuguacc cugacauuga ugucguguuc     3000

guaaaagccg aaaacgugac agacuuucgu cacgauaaua acuucaucaa gguucgcagu     3060

cucaaucauc accaucacgc aaaagacgcc uaucugaaca uugucguggg gaauguguac     3120

cacgagaagu uuacccggaa cuucagagcu uucuuuaaaa agaauggagc gaacaggaca     3180

uacaaccuag ccaagauguu caauuaugac gucaaaugua cuaacgcaaa ggaugguaag     3240

gcuugggacg uuaagaccag cauggauacg gugaauaaaa ugaugaccuc gaacgacgug     3300

cgaguaacaa agcggcugcu ugaacaaucc ggcgcccucg ccgaugcuac ugucuacaaa     3360

gcaaccgugg ccgcgaaggc uaaggacggg gccuauaucg gaaugaaaac aaagucuagc     3420

guuuuugcag augugacuaa auacggcggu augaccaaga uuaaaaaugc cuauucaauc     3480

auuguccagu acaucgggaa gaagggcgag gugguuaaag agauugugcc ccugccaauc     3540

uauuugacga accgcaauau aacagaccag gaucugauug acuacguagc uaguaucacc     3600

ccucaggcaa aggauaucuc cauuauauac agaaaauuau gcaucaacca acucgucaag     3660

gugaauggau ucuauuacua ucugggcggg aaaacuaacu cuaauauuua caucgacaac     3720

gccguggaag ucguugugcc cgcgaauauu gcuguauaua ucaaggugcu ugagaaguuu     3780

gcccuguugc auaaagauaa caauaacaua aaggcaagca cagucaccac uaggaucuac     3840

aacgaaggau ccaccaauau ugugucaauc acagacaaag acggccucau uguuuaugau     3900

uaccugauga gcaagcuacg gacgccacug uacaugaaaa ugaaggguaa caagguggac     3960

gagcuuagua ccgucgggcg aucuaaauuc augaauauga cucucgagga acaguccauc     4020

uaucuguugg agguguuaaa ccugcucaca aauagcaaga ccacauuuga uguuaaagcc     4080

cugggaauaa cugcuucgag aucaaccauu ggcgugaaga uccacacgcu ugacgaguuc     4140

aaaaucauua accagucuau cacaggucug uacaguaaug aaauaaccau ugucuga        4197


<210>  3285
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3285
auggagaccc agacaagcaa ccaacugauc acuucccacc ucaaggacua ccccaaacag       60

gauuauuucg ugggccuuga cauugggaca aauucugucg gaugggccgu uaccaacaca      120

ucauacgaac uguugaaguu ucauagucac aaaauguggg guagcagauu auucgaggaa      180

ggcgaguccg cugugacuag gcggggguuu cgcucuaugc gacguagacu ggaaaggcgg      240

aagcuacgcc ucaaacugcu ugaggagcug uucgcagaug ccauggcgca gguagacucg      300

accuuuuuca uaagauugca ugaaucaaag uaucacuacg aggauaagac aaccggacau      360

agcaguaaac acauccucuu uauugacgaa gauuauacug accaggauua cuucacagag      420

uauccuacca ucuaccaccu gaggaaggac cugauggaga auggcacgga ugacauucgg      480

aaacucuuuu uagcugugca ucacauccug aaguaccgcg guaacuuccu uuaugaaggg      540

gccacuuuua auuccaacgc auucaccuuu gaggaugucu ugaaacaagc ccuggugaau      600

auaacauuca acugcuucga cacuaauucu gcuaucagcu ccauuucaaa cauccuaaug      660

gaaaguggaa agaccaagag cgacaaagca aaggccauug agcgacucgu ugauacauac      720

accguguuug acgaagucaa uacgccagau aaaccgcaga aggaacaggu gaaagaggac      780

aagaaaacuc ugaaggcuuu cgccaaccuu guacugggcu ugucugcgaa ucucaucgau      840

cuguuuggcu ccguugaaga cauagaugac gauuuaaaga aacugcagau ugugggggac      900

acauaugaug agaagagaga cgaacucgca aaagucuggg gagacgagau ccauauuauc      960

gaugacugua agucagugua cgaugccauc auucuuauga gcauaaaaga acccggucug     1020

accaucaguc aaucuaaggu gaaggcuuuc gacaaacaca aggaggauuu ggucauucua     1080

aaaucccugc ucaagcugga caggaacguu uauaaugaga uguuuaaaag cgauaagaag     1140

ggccuucaca acuacgugca uuauaucaaa caggggcgga cugaagagac aucgugcucu     1200

cgugaagacu ucuacaagua caccaaaaag auaguagagg gacuggccga uuccaaagac     1260

aaggaauaua uuuugaacga gaucgagcuc cagacgcugu uaccucugca gcgcaucaag     1320

gauaauggcg ugauuccaua ccaacuacac cucgaggagc ugaaagucau ccuugacaag     1380

ugugguccca aauuuccuuu cuugcauacc gugucagaug gguuuagugu uacagaaaag     1440

cugauuaaaa ugcucgaguu cagaauacca uauuacgugg gaccccugaa cacucaccau     1500

aauaucgaca acggcggcuu uagcugggca gucaggaagc aggcugggcg ggugaccccu     1560

uggaauuucg aggagaaaau ugaccgagaa aaguccgccg cggccuuuau caagaaccuu     1620

acaaauaaau gcacuuaucu guucggagaa gauguauugc caaagucuag ccuccuguac     1680

ucagaguuca uguuacugaa cgaacuaaau aacguucgca ucgacgguaa agcacucgcu     1740

cagggcguga agcaacaccu gauugauagu auauuuaaac aggaccacaa gaagaugacc     1800

aaaaauagaa ucgagcuuuu cuugaaggau aacaauuaua uuacgaaaaa gcauaaaccg     1860

gagaucaccg ggcuggacgg agaaauuaag aacgaucuca cuuccuacag ggacaugguc     1920

cggauccugg gcaauaacuu ugaugugagc auggccgagg acauaaucac ggauauuaca     1980

aucuucggug aaucuaagaa aauguuacgc cagacccuga gaaacaaguu ugggucacag     2040

cuuaaugacg agacuauuaa aaaguugagu aaacucaggu accgugauug gggccggcug     2100

agcaagaagc uacugaaagg aaucgacggc ugugacaagg cugggaacgg agcccccaaa     2160

accauaauug aacucaugcg aaaugauucc uauaaccuga uggagauccu uggugacaag     2220

uucucuuuua uggagugcau ugaagaggaa aaugcaaaau uggcccaagg ccaggucgug     2280

aacccucacg auaucauaga cgagcuggcu uuaucgccag cgguuaagag agcagugugg     2340

caggcccuca ggaucguaga ugaagucgcu cauauuaaaa aggcccugcc cagccgcauc     2400

uucguggagg uugcacggac aaauaagucc gagaaaaaga aaaaggacuc aagacagaaa     2460

aggcuuagug aucuguacuc ugccauuaag aaggacgaug uguugcaaag cgggcuccag     2520

gacaaagaau uuggagcucu gaaguccggc cuagccaacu augaugacgc agcgcugcgc     2580

agcaaaaagc ucuaccugua uuacacccag augggucgau gugcuuauac ugggaauauc     2640

auagaccuua accaguugaa uacagauaac uacgacauug aucacaucua cccucgguca     2700

cugacgaaag acgauucuuu cgacaauuua guccucugcg agcguaccgc caacgcaaag     2760

aaguccgaua ucuauccaau ugacaauaga auccaaacua aacagaagcc cuuuugggcc     2820

uuccugaaac accagggccu uauuagugaa aggaaguacg agcgccugac acggauagcu     2880

ccgcuaaccg ccgaugaccu cagcggauuu aucgcaagac agcuggugga gacaaaccaa     2940

ucuguaaaag cuacuaccac guugcugagg cgauuauauc cugauauuga cgugguuuuc     3000

gucaaggccg aaaacguguc cgauuuccgc cauaauaaca auuuuaucaa ggugcgguca     3060

cucaaccacc aucaccaugc gaaagacgcc uaccugaaua uugucguugg caacguguau     3120

cacgagaagu ucaccagaaa uuuuaggcuu uucuuuaaaa agaacggggc uaaucguaca     3180

uacaaccugg caaaaauguu caauuaugac gugaucugua cuaacgccca ggauggaaag     3240

gcuugggacg uaaagaccag uaugaauaca gucaaaaaga ugauggccag caacgaugug     3300

cgcguuacuc ggagauugcu cgaacagucg ggugcacugg cggacgccac cauauacaag     3360

gcuuccgugg ccgcaaaagc uaaagauggc gccuacaucg ggaugaagac gaaauauucu     3420

gucuuugcag acgugacaaa guacggaggc augaccaaga uuaaaaaugc cuauagcauc     3480

auuguucaau acacugguaa gaaaggggag gaaaucaagg agauagugcc ccuaccaauu     3540

uaucugauca acaggaacgc uacagauauc gagcuuauug acuacguaaa aucagucaua     3600

ccuaaggcga aggauaucag uauuaaauau cgaaagcucu gcaucaauca gcuggugaaa     3660

gugaacggcu ucuacuacua uuugggaggc aagaccaaug acaaaauuua caucgauaac     3720

gccauagaac uggucguucc ccacgacauc gcaacuuaua uuaaguuacu cgauaaguac     3780

gaccugcuuc ggaaagagaa uaagacccug aaggcuuccu cuaucacaac gagcauuuau     3840

aacaucaaua ccuccacugu gguaucauug agcaacaaag uggggauaga cgucuuugau     3900

uacuucauga guaaacuccg cacaccacug uauaugaaga ugaagggaaa uaaaguggac     3960

gaacuaucuu ccaccggcag aagcaaguuu auuaaaauga cacuggagga acagucgauc     4020

uaccuucucg agguucugaa cuuguuaacu aauucaaaga ccacguucga ugugaaaccc     4080

cuggguauca cagggucuag gaguaccauu ggagucaaga uccauaaccu cgacgaguuc     4140

aaaauaauua augaauccau cacuggccug uacagcaacg aggugaccau uguuuga        4197


<210>  3286
<211>  4200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3286
augaagacca uccugggccu cgaccuuggg acaacuagca uuggaugggc ccuggugaaa       60

gaggcugaac ccuucuccga ugagaagucu gaaauaguca gauugggugu uagggugauc      120

ccuuuaacgg uagacgagcg gaccucauuu gaaaacggca aaaguauuac aacuaaugca      180

cugcgcaccc agaagcgagg gaugcguaga aaccuacaaa gguacaaacu ccggcgccag      240

gcccugaucg aggagcuuaa gagcacagga uggauuggca augauuucau cuuuuccgaa      300

accgguccag ggucuacuuu cagaacacug agguugcggg cgaagucggc uucagaggaa      360

gugacccucg gagaccuggc ccgcauacug cuccagauca acaaaaagcg aggcuauaaa      420

agcaguagaa agauggucuc cucugaugac ggccaggcaa uugaugggau ggccguggcu      480

aaagagaugu acgacaaggg auuaacgccg ggugaguaug uuuaccaaag gaugcuggaa      540

ggcaaguaug caauccccga guuuuacaaa agcgaucuuc aggaagaguu cgaccggauu      600

ugggccuugc agaaguccuu ucacaucgaa cugacugaug agcuauaucg uaaacucuca      660

gaccgcaaua agagucagac cugggcuaua ugcagagaug agcuggggau ucaaggacuu      720

aaaagcucuu ucaucggcaa ggaaaggcug aaagaggccu accgguaccg acauuuggcg      780

gcaacacagg aagugaacau ggagguccuc acuugugccc ugcaggaaau uaauggucag      840

aaggcuuccu uaucagggcu gcucagcaag aucagugacc gcucuaaaga gcuuuaucug      900

aacaauuuga ccgugggaca aaugcuacug aagagaaucg aggacaaccc uaauaacucc      960

cucaaaaaua uuguauuuua caggcaggau uaucuggacg aauuugagcg gauauggaca     1020

guucagagca aguaccacac cgaacuuacg caugagcuga aaaagcgcuu gagagauauc     1080

auuaucuuuu aucaaaggcc acucaagucg cagaaaggcc ugauaucuuu augcgaacug     1140

gagacuaagg acauugaggu ggccaucgau ggcgucaaaa agaugaaaaa caucgggccc     1200

cguguguguc cucgguccuc accacuauuc caggaauuua agauuuggca gacaaucaau     1260

aacauuuucg ugaacggagc agcucucgac gaugaggaca agagugucau agccaaagaa     1320

cugauguuca aggauaaacu uaaggacagc gauuugcuga aacuccuguu uaagaccacu     1380

aagggucaau ccguuaauuu caaagaggug gacggcaacc gaacaauggc gacccuuaug     1440

aaggccugcu uuaaaaucgc agagaaucug ggguacgacg auauuggauu gucuaagcuc     1500

agcucagaag accagauggu aagaaucgag ggcaucuuca aagaacaggg uuuuaagacg     1560

ggguuccugc acuuugauag uuccuuagcu agccccgagu ucgaaaacca ggacucuuau     1620

aggcuguggc aucuacucua cucauacgag ggcgauaaaa gugccagcgg aaaugagucc     1680

cuggugaagu guauuaugga ccuuugcucu auggaugaag gcucggcuaa gauauuggcc     1740

agcuguaccu uucugccuga cuaugggucc cucucagcaa aagccaugcg caagauccug     1800

ccauacauga aagauggauu agaguauaca gcugcgugcg caauggccgg uuaccggcac     1860

aguaagagau cucugacuaa agaagaacuu gacaccaagg aauauagcga uagguugucc     1920

cuccugccga agaacucacu acgcaauccc gucguggaga aaauucugaa ccaaaugauc     1980

aauguuguga acagcgucau ugacguguac ggcaagccug augagauccg aauagaaaug     2040

gcucgggagc ucaaaaaguc ugccaaagaa cgugaggaag cacagagaga caucgccucc     2100

aguacagcug agagccagaa gguaauugag auccugaagg gggaaccauu cuuucuucag     2160

uuccccacua ggaaugacau uauccgcuau cgguuguacc aagagcuggc ccagaacgga     2220

uuuaaaaccu uauacucuaa uacauauaua ccuaaggaua aacucuucuc caaggaguuc     2280

gacauugagc acaucauucc acaggcacug aucuuuaacg auucauucgc gaauaaaacc     2340

cuugagagua gacaguguaa ccuggaaaag ggcaaggaga cugcuaugga cuacguuaaa     2400

agcaaguaug augaaacggg ugugcaaaca uacugcgaca gggucaacgg guugauaggc     2460

aaaggaaucu cgaagaccaa agccaagugg cuccugaugg agggcaaaga uauucccgaa     2520

gacuuuauca aucgagagcu acgcgauucc caguauauug caaagaaggc ccgggagauc     2580

cuggaaucuc ucgugaaauu cgucgugccg acuaccggga gcguuacagc uagacugagg     2640

gaggacuggc agcuugugaa cguaaugcaa gaauugaauu gggagaagua cgcccgucug     2700

ggauuaaccg guguccgcga agauaaagac ggccggagaa uaccuuuuau ucaggauugg     2760

acuaagagga acgaccaucg acaccaugca auggacgcuc ucacaaucgc cuucacgcgg     2820

cgccaguuua uccaguaucu gaaucaccuu aacucaaaaa uugauguggu uaguugggac     2880

aagaaggauc uggaccuaag agauuacgac cucgaggaua ucaaauucgg gaaucugucc     2940

gcgggagaca gguacggcau ugugaaggcc uugcaagaua aauuucugua uaaggacggu     3000

aacgauaaau accgguucgu gcccccaaua ccuuuagacg aguuucgaag acaggcuaag     3060

gaacagcucu cugacauccu gauuagcuuc aaggcaaaga auaaggucug uacccgcaac     3120

guaaauguga cuaaaaacaa agggggcgcc aauaggaaag cucagcuuac accccgugga     3180

ccacugcaca acgagaccau cuauggcucc ucauuggaau acguuacaaa ggagaaugaa     3240

aagauuggga gcaguuuuga uacugagcgg aucaccacgg ucugcaaaaa gaaauucaga     3300

gacgcccucg caaggcgccu ggaggaguuc ggaggcgauc ccaagaaagc guuuaccggu     3360

aagaacucuc cugaaaagaa cccaauaugg guggacgaac aucacuccga gcaagugccg     3420

gccaaagucc gaacaguuac uauggggcag agauucaccg gacggaagcc caucgaugcu     3480

acacuaaaaa uugagaaggu gaucgacaaa aggauucgcg aaauccugca ggcccgucuu     3540

gaugaguuug acggcaaggc agcuaaagcc uucagcaauc ucgaugaaaa cccuauaugg     3600

cugaauaagg agaaggguau ugcaaucaaa agagugacug uaucgggccc agccaacccc     3660

gucccugugc gguuuaagag ggacaaagau gggaagccaa ucauugacga ugcuggaaaa     3720

accauaggcg cggacuucgu uacgcccggg aauaaccauc acaucgccau uuuucgcgac     3780

ucaucuggaa aguugcagga acauccggug aguuuccugg aggcaacaau cgcuaagucc     3840

cacggcuuag augucauuga ccgaaauuau aacaaagaug aggguuggga auuucucuuc     3900

acccugaagc aaaaugagua cuuuguguuc ccuaacagcg aaacuggguu caauccacuu     3960

gacuaugauc ugacagaccc cagaaacuac gccgagaucu cuccuaauuu guaccggguu     4020

cagagcauau ccaccaacga uuauuucuuc aggcaccauc ucgaaacuac cucagagaaa     4080

aauaacaguc uguacggaau cacauggaag cgcauuagaa acgccagcgc ucuagagggc     4140

cuggugaaag uaaggaucga caaucuuggu cggauugucg cagugggcga auaugauuga     4200


<210>  3287
<211>  4200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3287
auggagaccc aggccacagc ucaacugauc agcggccacc ucaaggggua ccccauuuau       60

gacuacuucg ugggacuuga uauagguacu aacuccgucg gcugggcagu uacgaauaga      120

aucuaugaac uguugaaauu uaggucucau aagauguggg ggucacgguu auucgaggaa      180

ggacagaccg ccgugguacg ccgaggcuuu cguaguauua gaaggcggcu ggagcgcaga      240

aggcuacggc ucaaacugcu ugaagcgcug uucgcugacg ccauggcacg cguggauagc      300

acauuuuuca uccgauugag agaguccaag uaccacuaug aggacaaaac uaccggucau      360

aagucuaagc acauucucuu uaucgaugac aacuacacag aucaggacua uuucgccgaa      420

uacccuacca uauaccaucu gagggcugau cugauggaga augggacuuc ggacauccgg      480

aaacucuuuu uagcagucca ccacauucug aaguaucgug gaaacuuccu uuacgaaggc      540

gccacauuug auucaaauag caguuuggac accgugcuga aacgcgcucu agagcagauc      600

gaguuccaua uuuuuaacgc cacguccgaa gaugcgcucc acucuauccu ggacauaguu      660

aaacuucaag acaaaacuaa gagcgauaag gcaaaagcca uugagucccu gguggacacc      720

uauaucacag auaaggucga aucaaguugc acugacaaag agaagaaaga uuugcucaag      780

gcugacaaaa agagacugag ggccuucgca aauuuaauuc ugggccucaa cgcuaaucuu      840

accgaucugu ucgggacaau cgaagagauc gaggaagacu uugugaaguu gcagauuacc      900

ggagauacgu acgaggaaaa acgggacgau uaugccaagg uaugggguga cacuauacga      960

aucauugacg auuguaaaag cguuuacgac ucuaucauac uaauguccau uaaggagcca     1020

ggccugacaa ucucacagag caaagugaag gcguucaaca agcauaaaga agaucucgcc     1080

auccugaagc gccuucugaa aagugacaag uauauuuaca aaaauauguu uaaggcagau     1140

gagaaggggu uggcuaacua cguccacuau aucaaacagg gaagaaagaa ugagaccggc     1200

ugcucuugug aagacuucua caaauauacu aagaaagugg uggagucccu cccggauagc     1260

ucggacaagg ccuacauucu gaacgaaaua gaguuacaaa agcugcuacc ccuccagagg     1320

aucaaagaua auggugucau uccuuaucag cugcaccuug aagaguugaa ggcuauccug     1380

gagaaaugca aggacacauu uccauuccuc aacaccguuu cugauggguu uacgguggcc     1440

gaaaaacuga ucaagaugcu ugaguuccgg auucccuacu auguaggacc ucugaaccca     1500

ucccauucaa uagacaaagg cggcuuugca uggguggaac gcaaggagcc cgggcagguc     1560

accccuugga auuucgagga uaagaucgac agagaaaaga gugccgaggc uuuuauuaag     1620

aacuugacaa auaaauguac uuaccucauc ggagaagacg ugcugccaaa gagcuccuua     1680

cuguacucug aguucaugcu acucaacgaa cugaauaacc uuaggauuga ugguaaaccg     1740

uuggagcaag cgguuaagau gcaccugauc gacgcagugu uuaagaaaga gcauaagaaa     1800

cagaccaaga aucguauaau cgaguuccuc aaagauaaca auuauauucu gggcaagcac     1860

aagcccgaag ucgacggguu agaugaaagc aucaaaggag accuggccuc auaccgggau     1920

augaagcgaa uccuuggcga cgauuucgac gugaguaugg cugaguccau uauaacaagc     1980

auuacuaucu uuggugaauc uaaaaacaug uugagagaga cccucaggaa gaaauucggg     2040

ucaauucuga gugaggauca gauccgcagc cuagccaagc ugcgguauag agacuggggc     2100

aggcucucca agaaauuucu gauaggaauc gauggcugcg uaaaggggga agacggaaca     2160

ccugguacca uuaucgacau gaugcgcaau acuucucaua accuuaugga guugcugggc     2220

gauacguucu cguuuaugga cguuauuauc gaagagaaug auaaauuaac aaaggggcaa     2280

guggucgacc cacacgaaau aauugaggag cucggacuga gccccgcagu gaaacgagcc     2340

gucuggcagg cccuucggau cguggaugaa guuauccaca uuaagaaagc ucugccuucc     2400

cguaucuucg uggagguagc aagaaccaac aaggacgaaa agaaaaagaa agauucaagg     2460

aagaaacgcu ugcaggagcu cuacaagagu auucagaagg agucuccaga auggcugagc     2520

gucacucggg acgaguccga uaaacuaaua ucaaaucugg acagagcgga ugacagcucu     2580

cucaggucca agaaacugua ucuuuacuau acccaaaugg gccgauguau guacaguggu     2640

cgcgugaucg acuuggauca gcugaacaca gacaaguacg auauugacca uaucuauccc     2700

cggagcuuaa ccaaagauga cucuuuugau aaccucguuc ugugcgaaag acaggagaau     2760

gcuaagaagu ccgacaacua cccgauugau ccugacaucc agaaugccag gaaacccuuc     2820

uggacuuuuc uuaaggauca aaaccugaua ucaagucgua aauaugaacg ccuaacacgg     2880

gugcucccac uggaggcaga cgauuugacg ggguucaucg ccagacagcu ggucgagacc     2940

aaucagagcg ugaaggcugu aacuacauua cucaggcgac uguacccugg cguggacguu     3000

gucuuuguga aagccgaaaa cgugaccgac uuccggcacg auaaugacuu uauuaagguc     3060

cgcucgcuua accaucacca ccaugcaaag gaugcuuauc ugaauaucgu ugugggaaac     3120

guguaccacg agaaauucac aagaaauuuc aggaaguuuu ugaaaaagaa cggcgccaaa     3180

cggacuuaua aucucgcgcg aauguucgac uuugauguaa uguccaccaa cgccaaggac     3240

gggaaggcuu gggauauuaa aacgucuaug aauaucguca agaaaaugau ggcaagcaac     3300

gacgugagag uuaccaagcg ccugauggaa cagaaaggag cccuaucaga ugcuacagug     3360

uacaaggcca gugucgcagg uaaagcgaag ggcggggccu acauaggaau gaagacuaaa     3420

gacuccgugu ucucugaugu uagcaaguau ggcgguagga ccuccauuaa aaacgcuuac     3480

ucaaucgugg uagaguauac agggaagaaa aaugaaauca uuaaggagau agucccccug     3540

ccaguguacc uuaucaaccg uaauaagacu gaccaagagc ucauucauua uaucgugagc     3600

auuauccccc aggccaaaaa cgucaguaua aucuacaaga aacuguguau uaaucaguug     3660

guuaagguga acggcuuuua uuacuaccug ggaggcaaaa ccaauaacua uuucuggauc     3720

gauucugcaa uucagguagu gaucgcugac gaauggauac cuuacuuaaa gguccucgag     3780

aaguuugacg ugaugcggaa acaaaauaag gaacugaaag uugggacggu guccacaagc     3840

auucagaacc agggaaagaa agucgugauc aagauccuug aggaaagaaa uguugaguuc     3900

uuuucguauc uggugggcaa guugcacuca ucuauuuaca guaaaaugaa ggguaacaaa     3960

gccggggagc ucgcaaccaa gucccuggaa aucuucguca auaugagccu agagaaccaa     4020

ugcauauauc ugauggaacu ucucaaucug uugacuaaca aaaaggcccc agauguaaaa     4080

ccguuaggaa uuacagcuuc uaggggcacc aucgguagca agguguccaa ccaggacgag     4140

uuuaagauua ucaaugaguc aauaacuggc cuguacagua acgaaguuac cauuguguga     4200


<210>  3288
<211>  4200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3288
augaaccuga gaaauaccac agacgaguac aacaucggcc ucgauauggg gacuaauagc       60

gugggauggg ccgucacgga caaggcuggu gcaauugccc acuucaaggg caagcccacc      120

cuugggucca ggcuguuuga ugcggcucag acagccgcag gagcccggau acaucgcucu      180

caacgacgua gauauguuag gcggcgcugg agauuggacu uacugcaguc acuauuccag      240

gaugaaguga guaaaguaga cagcgaguuu uucugcaggc uccggcaguc ccgccuguac      300

gugggcgauc gagaaaaggg ucacgcugac uauacuuggc cuaucuuuaa cgauucugac      360

uucaaugagg ucgauuacua uaaaagauuu ccaaccauuu accaucuuag gaaguggcug      420

auggaaacag acgagaaggc agauauccgg uugauuuauc ucgccaccca caacaucgug      480

aaacaucgug ggaauuuccu gcgcgagaac aauaagucgc ugucaagcag agacgcuaac      540

ccggcccaag cgguuaaaag ucucagguua gcacugaaug acugguguuc cgaacgggau      600

uacgagugcg ccuggaaggg agaagaggac gauggcgcua uagacgaggc cauccuuaac      660

guguuggcag auggcgaaaa agggcgaucu gaccaggcua aggagauugc caagcugacu      720

ggagucgauu guggugacgc gaaacagagc aagcagugca augaagccau cgcaaaagcu      780

auugugggcc uaaagguaga gaugaaaaac aucuuugggg aguuccccgc cgaaaagaca      840

uccuacauau aucucucaag ugaugaggac guugaagcug ugcgcgagag cuguccugau      900

gacucuggag aacuguuuca agcccuuugc aaacuguacu ccgcauauau uuugcaggag      960

cuccugucau acgccgaccc aagcggccag accaucagug cuaauauggu cgcgaaguau     1020

aagcaguacg guagagauuu aaggcugcuc aaagaacuua cgcggacuua ugcaccccaa     1080

aaguacgacc uguucuuucg cggggagacc uacgccggau cuuccgauua ugugguggac     1140

gaggcuagag gcuacacaag guauaacuug ggcacuacca aagccaacua cgaugacuuc     1200

caaaagagcg ucaaagaacu auucaagggg acagaugcac ugcaggacga gcguuaucaa     1260

gaaaugaugg uugccuuugg agagcagaag uuccuccggc gacugaaaac cucggauaau     1320

gguucuauuu acuaucagcu ucaccuggaa gaguugcaca cgauccucga gaaccagggc     1380

aagcauuacc cuuuucuguu auccgaagag gacaaaaucg aaucacuggu gaguuucagg     1440

auuccauacu auguagggcc ccuaacuaga aagaaugcug ccauaggaca cgauggcaaa     1500

gagcgcuuug cauggagcuc ccggaaggac gguauggagc augcggugau cacaccuugg     1560

aacugggaag agauuaucga uagagacaag ucugcugaag acuucauaau gaggaugacc     1620

ggggauugua cuuaccucca aggcgagcca guccugccga aaugcagccu uuuguaugaa     1680

gaguuuugug cccugaauga guucaacgga gcacacugga caguggacgg cgaugacgaa     1740

cgccgauuug augccgcuca gcgggagcgu auuaugugcg accucuucag aaggacccgc     1800

ucaguuacgu acaauaaggu gagugauugg cuggaacgga aauauaacuu uaccggggcc     1860

agagucaggg gaggucaggg cgagcacggg uucgaaucca aacuuggaag cuacaucuuu     1920

uucuguaagg acguguucaa gacagauggc cuggacgagg cagauuauac uaugaucgag     1980

cagauuaucu uguggucuac ccucuuugaa gaccgagaua uucugaagcg caaaauagag     2040

gaggcuuacg guggggaccg guuaacauca gaacaaauca agguaauuug caaaaagaga     2100

uucacuggcu ggggaaggcu gaguaaacgu cuacucaccg acaucaaggu uaauacagau     2160

accggccgca aaagcaucau ggacgugcug cgggaaggga accccaauaa cggacagaga     2220

uccaggacua uggucuuuau ggaggugcuu cgagaugagg acuuggguuu ccagaaggcc     2280

auugauggcc ugaaucggga cuacuuucgc gaacauaaca augggcucau ggaugucaac     2340

gaccugccug gaucuccagc gauaagaagg ucgaucaacc aggccgugcg gauuguugau     2400

gagaucgcua gcauugcacg aaaggaaccc acgaauaucu ucauagaggu gacaagagag     2460

gaagacgauc cucgcaaaaa gggcaaaagg accaagcguc gguaugacgc caucaaaucc     2520

gcuuuagaga aguuuaagaa agacgaucca ucacugugga aggaacuuug cgagaaagcc     2580

cccggugaca uggaugaaag auugagucuc uacuucaugc aaagggggaa guguauguau     2640

ucuggccgcc cgauuaaaau cgaggagcug cacacuggaa aguacgaagu agaccauauu     2700

aucccucgaa gcuauauaaa ggaugacucc uuugagaaca aaguccuagu guacagagaa     2760

gagaaucagc acaagaccga ugcacugcuc auugacucaa gcauccggca gaaaaugggc     2820

ggguauugga ggaugcugca ugaugcgaaa cuuguuggag acaagaaguu ccgcaacuug     2880

uuucguaauc acauugauga caaggccaug aaaggcuucg uggcuagaca gaucgucgaa     2940

acaucucaaa uggugaagcu gguacagacc uuauuugagg cccgguaccc caacacuagg     3000

auagugccag uuaaagcagg uaucucccac gaucuccgcg aggcugccgg gcuggucaag     3060

ugccgagaag caaaugacuu ccaucacgcc caugacgcuu uccuugcgug uagaauugga     3120

cuguuuaucc agaaacggca cccuagugug uacgauaacc ccauuggcuu ggccaggauc     3180

augcgcgacu augcaagaau gcaagcuagg gaguacaaga aaagccacug ccucgcccau     3240

acacaggguu ucauagugaa uagcuuucug uccucaggcu ucgaugaaga gacgggggaa     3300

auuuuuaagg acgauuggga cgccaaggcu gaggucgagg gaauccggcg uguucuaaac     3360

ugucgccagu gcuucaucag ucgaaugcca guggaaggcg auaccagaua ugggggaagg     3420

uacuggggcg agacuauuua uagccccaaa gguaagcaca aacaugaaau cccucugaag     3480

caggggcucg acccaaaucu guacggaggc uauucggauc uuuuguacuc cuacuuucug     3540

auuuaugagg caucugacgu gagcucaggu gagcggguau uacgcuucau aggcaucccg     3600

acaaguauug ccgcgagaau ggcuaaccag aaaaggcucg aagaguacgu cucccggcug     3660

gcccuucagg aaaaucugga gcacgugcga augguuagaa agaucaugaa gaaacagcua     3720

auugaagugg auaacugucg ccuccugguc accgcagccg acgcugugac aaaugcaacu     3780

gaguuggccu uuucucugga ugagaugaag aucauaucua ccugcgcgaa auguaucaac     3840

ggggcugacc ccuccgccau ugacaagauc ggaaacgcac aggauucauu cuuugacaau     3900

uuaaaaaggc ggggcagcga ucguugcgcc cuccuguuca gucaacuuaa gcuggacaag     3960

uugucucaga acuucuauau gauuuccagc gaugacaaau cgaagauccu cauaucaauu     4020

cuggauagaa ucagggcuaa aucuaauagu guugaccuau cccugguagg gggagccaag     4080

caugcagcuc agaugaaauu ugauuucaac gccaaacuua auaacccugc gagcucuuuu     4140

gugcucaucg accagagcgu cacgggcaug uucgaacgcc ggacccgaau uggucuguga     4200


<210>  3289
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3289
augaaccuga gaaaugccac cggcgacuac agcaucgggc ucgaucuugg aacaaacucc       60

guggguuggg cugucacuga cucuaauggc ucaauggcac acuucaaggg gaaacccacg      120

cugggaagua gguuguuuga ugccgcggag accgcugccg gcgcacggca gcaucgcggu      180

caacgacgua gauauguuag gcggcgcugg agauuagacc ugcuacaggg gcuauuccag      240

gaugaaguga acaagguaga cagcgaguuu uucauuaggc ugcggcaguc ccgccuucac      300

guggaagauc gagccgaggg acaugcuggc uacagauggc cucuguuuaa ugacacagau      360

uucacugaaa aagacuauua caagagguuu ccaaccauau aucacuugcg gaaauggcuc      420

auggagacag augagaaggc agacauccgu cugauuuacc uggccgcuca uaacaucguc      480

aagcaccgcg gcaauuuccu cagagaagau aacaaaaauu uaucuucgca caacgccaau      540

ccggcggagg cagugagguc acugcggcaa gcccuugacg acuggugcgc ugaacgaggg      600

uauguuugug ccuggaccag cguggaugac uuggcagagg cugauaguca uauucuggac      660

auccuaacua acuccaagga gcgcagagcc gaucaggcga aagaacucgc caagcuguuu      720

ggagucgacu gcggugauac aaaacagucu aagagcugua auuccgcacu ugcuaaggcc      780

gugguaggcc ugaaaaccga guugaaggac guuuucgggg aauuuugcgc ugagaaaacg      840

gccauacacc ucucaaguga ugacgaugug gaagcaauca gggacgccau ucccgacgau      900

agcggagagc uguuccaggu cuuaugugcu guguacucug cguacgugcu gcaagagcuc      960

cuuuccuaug caccuggcca gacuaucuca gccaacaugg ucgcuaagua cgaccaguau     1020

gguaaagauc ugaaguugcu caaaaaucug gcccggaccu acguuagccg cgaaaacuau     1080

gacgcauuuu ucagagggca gacauacgag gauaguaacg acuauaaugu ggauaaggcc     1140

caaggauaca cuagguacaa ccucggcacc acaaaggcuu cuuaugacga uuucaaaaag     1200

uccguagaaa aacuguuuaa gggcacccag gccgagcagg acaaacguua ccaagggaug     1260

augaagcggu ucgcagaaga gaaguuucuu cgaagacuga aaacgagcga uaauggaucg     1320

auuuauuacc aguugcaucu cgaggaacug agggcgaucu uaaagaacca ggguaaacac     1380

cacccauucc ugcuagagga agagaagaaa auagaaucuc ucguguccuu ucgcauuccc     1440

uauuacgucg gcccucuguc aaguaagaau gcuagccuug accauaaggg gaaacagcgg     1500

uucgccuggu ccgagagaag gccaggaaug aaggauacug ugaucacacc cuggaacugg     1560

gaccaaauua ucgacugcga ucagucugca gagaaauuua ucuugcgcau gaccggcgac     1620

uguacuuauc ugcaggguga accuguucuc ccaaagugca gccugcuuua cgaggaauac     1680

ugugugcuga augaguugaa cggggcccga uggacaauug auggcgacga uuggcggcgu     1740

uucgacgucg cucagagaga gggaauacuc cacgaucugu uuaggaaaac ccgcucagug     1800

gccuauaaua agguagcaga cugguuagaa cgggaggaaa acugggcuaa ugcccauguu     1860

agaggcgggc aaggagagaa caguuucgaa uccaagcugg guagcuacau cuuuuucugc     1920

aaagaugugu uuaagacggc ggcccuagac gaggcugauu auccgaugau ugagcagauc     1980

auacucugga auacccuguu cgaagacagg gcaauucuua aacgaaagau cgagcaggcc     2040

uacggcgaac accauggggg aaaauugaca gaugagcaga ucaaggucau uuguaaaaag     2100

cgcuucacug gcuggggucg gcugucuaga aagcuccuga ccgggaucaa aguggacgcu     2160

gacacaggca gggucucaau uauggaugug uuacgugaag gaaaccccaa ugagggccaa     2220

cgcagucgga cuaugguucu gauggaggug cuuagagacg aagauuuggg guuucagaag     2280

aaaauagacg auuucaacaa ggccuauuuu gcaggacagg gugagggccu caugagcgua     2340

aacgaccugc cugggucccc agcgaucagg cgaucuauua aucaggccau ccggaucguc     2400

gaugaaauug cuucgauagc cggaaaagca cccagcaacg uguucaucga gguuacccgc     2460

gaagaggacg auccuagaaa gaagggcagg cggacaaauc gaagauacga ggacauuaaa     2520

aacgcucuag aaaauuuuaa gaccgaggau caagaacugu gguccgagcu ccuggguaaa     2580

gccccaaacg accuugacga gcgcuuguca cuguauuuca ugcagagggg gaagugcaug     2640

uacaguggcc gucccaucaa auuagaagaa cuccacacug gaaaguacga aguggaucac     2700

auuaucccgc ggucuuauau aaaggacgau agcuuugaga auaaaguccu ggugugcaga     2760

gaagagaacc agaggaagac gaaugagaug cuuaucgacc cugcaauucg ccgaagaaug     2820

ggcgauuacu ggcggaugcu gcauaaagcc aaguugaucg gggacaaaaa guucaacaau     2880

cucuuuauga gggaucgcau ugacgauaag acacugaaag gauucguagc ucgucagcua     2940

guggaaaccu cccaaucagu uaagcugguc cagacucucc uggaggugag auaucccaac     3000

accaaaaucg ugccagucaa ggcgaauaua agccacgacc uucgggaagc cgcagauuug     3060

guuaaaugua gggaggcuaa cgacuuucau cacgcccacg augccuaccu ggcuugccgc     3120

gugggcuuau ucauucagaa gcgacauccu ucuguguaug caaaucccau cggucucucc     3180

agaguaaugc gggacuacgc caggaaacaa gcgggggaau uuaacaagac acacaguaug     3240

gcucauacca agggauucau ugucaacagc uucaugacuu cugaguuuga caaagagaca     3300

ggcgaaaucu ucugggaugc cgaggcagcc guggaagaca uacgcagagu ucugaauuau     3360

aggcaguguu uuaucucccg gaugccagug gaggauacgg gugcuuucug ggaccagacc     3420

auuuacucac cccguaguaa caagcgccuu gaacugccuc uaaaagauga ccucgaucca     3480

aauaaguacg gcagcuauuc gcgagagcag uuugcauacu ucuuuaucua ugaggccaga     3540

gacgcgaaaa aguccaagac uguccugagg uucgcucaag ugccgguucg ggugacaucu     3600

gccauugcac gcgauagcuc aaaauuggac gaauacgcca gugggcuggc uguacaggag     3660

ggauuagaau uuaccaagau cauaagaagg cggauucuca aaaagcagcu gcuugagcug     3720

aacggcgauc gauuggucau cacaggggcc aaagagguga gaaaugcaac ugaguucgcu     3780

uuuacccucg aggaaacggc ccugguggcg augugcuccg agaucgcccg cgaaggaguc     3840

cccucugaga uugcaagcga cguucagucc aucucaagcc aacuaaguga ucuguuccuu     3900

gcucucauug agaggggccu gaccauaggu gugcgguugc acgggcagcu gaaguuagga     3960

caguaucucc gucaauucga agacgccgcu cugagagaca aggcaaaagu aaucauuggc     4020

aucauuucua ucacaaacgg ugccacuaau acccuugauc uguccagcuu gaacggcucg     4080

aagaaugugg ggcaggucag gauaacacau aacaaacucc ugaaugacaa gucaacuucu     4140

uacgugcuaa ucgaucagag uguuaccgga auguuugagc gccggacgcg aauuggccug     4200

uga                                                                   4203


<210>  3290
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3290
augaagacca ucacagagga cuacauucug ggccucgaua uagggacuaa cagcugcgga       60

ugggccguga uggacaaaaa gaauagacuu cugagguugc gggguaaacg cgcuaucggc      120

ucccacuuau ucgaagaggg gcauucugca gccgaucgac guucauuuag aacgaccagg      180

cggcgccuga agagaaggaa auggcggcua cgccuccugg aagaguucuu ugcggaaccc      240

auggcuaagg ucgacccugc cuucuuuguu cgacuucacc agaguugggu gagcccacug      300

gauaaggaca ggaaaaagua ucaugcaauu guauucccga cagccaaaga ggaucacgag      360

uuuuaccaug acuaucccac uaucuaccac uugagagaug cucucaugcg ggaagaccgu      420

caauucgauc ugcgcgagau uuuucuggca auccaccaua uagugaagua uagaggaaac      480

uuccuccagg acaccccugu caauuccuuu guggccucua aaaucgaugu ugcuucguuc      540

uuaacaaacg ugaaucaggc cuacgcggac aaguauggcg aggagcagaa cuuuauugag      600

cugaaugaaa acaaugcagc cgcuaucgag gccgucauua gggguaagga caaagauaag      660

accacuaaac aacuugacaa ggcgaaaaag aucacaaaau ugcugcaguc aagcaccaac      720

aaguacgaaa agaaugugac gcggcagaua gcuaacgcca uuaucgggua caaaacucag      780

uucgagacca uucuaggaaa ggauaucgac aaaaaugaua aggcggacug ggaguucaaa      840

cucagugaug gcgacgccga ugacaagcug gaaaaccuuc ugccagaguu ugaugacaau      900

gaccaauaua ucguagcaga aauuaagaaa uuguucggcu ccauagcucu cucucagauc      960

guuuacgaga acaugacacu gagccagucc augaagugua aauauaagga ucacggggaa     1020

gacuacgauu uacugaaaca guauauugcc acucucucag accauaguca agugcgaaaa     1080

cuucuguugg cuuacgaucu auaugucaac aaucgccacg gaagacugcu cgaggccaag     1140

aaagaauuua agcaggacaa agcacugacc caggaugagu ucuacaagau cgugaaagag     1200

aaccuugacg auagccaacu ggcccagaag auauugcaga agauugacga agguaaauuu     1260

augcccaagc agagggugaa uaaaaacggc uacaucccuu aucaacucca ccagaucgag     1320

cuggaucaga uuaucgcuaa ucagucuaag uacuauccau ucuuagcgga agagaacccc     1380

gucaaagaac aucggaagca agcaccuuac aaacuggacg agcuaguucg cuuuagagug     1440

ccauauuacg uagggccgcu cauuuccccc gaugacaaga ggaaggacga gcagcagaau     1500

aaaaaccacg aaauguucaa guuugccugg augaaacgua aggaugacaa uggagcuaua     1560

acaccuugga acuucgccca gaaaguggau cgggagaccu cagcaaaucg auuuaucaag     1620

agaaugacgg ucaaggacac uuaucugauu ggcgaagaug ugcuuccagc caggagcuug     1680

cuguaccaaa aauucgaggu ucucaacgag cugaauaaca uccgcgugaa ugguaagcgg     1740

cuugaaccca aauuuaagca gaaagucuuc aacgagcugu ucaagcagaa gaacaaagug     1800

acaaagaagg cuuuuaagga guucaucuug cagaauaccg ggaugaaaac ugccgaaauu     1860

gacggacucg cagauccuac aaaguuuaac aguucucugu ccagcuacua ucauuuaaag     1920

ucgaccaaag uauucgacaa gcaacuggau gacccacagu accaggcgga uuuugagaaa     1980

auaaucgaau auucuuccau uuucgaggac gcugaaaucu accaagagaa gcuacacaaa     2040

gaaauuaagu ggcucacgcc cgcccaguuu aaagcaaucg ccaauuggag acugcagggc     2100

uggggcaggc uuucacgcaa guugcugguu gagcucaagg ggaaaggagg ugaaaacaua     2160

aucuaucagc uguggaccac acaaaagaau uucaugcaga uugugaacga gccggaguug     2220

aaaaaggcua ucgucaaaga aaaucaucag gccacugagg aaaagggcau ugagaagauc     2280

cuggaugaca guuuuaccag cccugcaaac aaaaaggcua uacgacaggu gauuaaaguc     2340

guggacgaug uugugaaggc cgcagggggc aaaaagcccg cccaauucgc uaucgaauuu     2400

acacgggagc caaaguggaa uccucaccuu uccaacauuc guggaacuaa auugcuccag     2460

guauacgagg acaccgcaaa gaaacugguc gaucagucuc uaacagacag ccugagagaa     2520

aauaugacca acaggaaucu ccugaaggau gaguauuucc uuuacuuuau ccaggguggg     2580

cgcgacgccu acacuggcaa aaagauagau aucgacgaua uucccaagaa auaucaaauc     2640

gaccacauuu ugccacaguc auucaucacg gaugacaguc uggacaaccg gguguuaaca     2700

gaaauagaga uuaaucagaa gaaaggaaag aaauacgcuu cccaucucuu cgcccccaag     2760

ccugcauuug gccugaacca aagcaucucu gagaugugga agaccuggca gaaagcgggg     2820

cuuaucucac agagaaagcu gaacaauuua cucuucgauc cagaccuggu uaaagauaag     2880

cagaaaaagg gauuuauuaa caggcagcua guggaaacca gucaaaucau uaaacugguc     2940

agcauaaucc uccaggacaa guucccggau acugagauua ucacagugaa ggccgguuau     3000

aauaccgcuc ugcgaaaaga acuuaaguug uacaaauccc gcgaggccaa cgacuaucac     3060

caugcaauug augcuuaccu gacugccauc ugcgacaacu uuuuauauca gguauacccc     3120

aauuuccggc cauuuuucgu guacggccaa uuuaagaaau ucucucagga uacagacaag     3180

cagaagaaag caguuaagua uuacgggcuc aaaaauuuuc ugcuuccucu gcuagaugac     3240

ucgacgggag augaagucau ggugaaccac accaagcacg uggucuucca uaaacagaga     3300

gacauauuca auaggcucau ccaagccuau aacuacaagu auaugcuggu uagccgugag     3360

acuacaaccg aaaauuccaa cauguuuaau augacaauuu acccccgcgg cgaacgggac     3420

agaaagaaaa cuaggaaguu gaucccuaaa gguaaggauc uggacccagc uauuuauggg     3480

ggcuacucag gaaacaccga ugcguacaug gugaucguga aaauagagaa gacgaaggaa     3540

accuuauaua aaguagucgg cgugcccaug cgagcccucg caagucugaa gaaagcucag     3600

aaggagggga auuacgacca ggaacuuaac aaaguucugg agccguugau uauguucaac     3660

aagaauggaa aaucuaaggc cgguaucaag ggcuuuacag uggucaaaga ucacgugcau     3720

uucaagcagg uugugaucga cggggauagc aaauuuaugc ucuauuccgc ccaauaccgg     3780

gcuaacgcaa agcagcugac ucuaucacag gagaccauga aaguagucac agacaauacu     3840

gaugacaugg aucaaaagaa cgccgacaag gaucagcugc uucucaaugc guaugacgaa     3900

auucuggaga aaguggacca guacuugccu cuguucgaua uaaacggauu ucgccagggc     3960

uuacacgacg guagagcuaa guucgccgca cucaaaacca gcgaaaagaa auauacgcug     4020

aagaaugugc uuaacgggcu gcaugauaau uuggucacac caaaccucaa gaauaucggc     4080

auuaaaaccc ccuuuggacu gaugcaaucu uccaguggca ucacucuaag cccuaacgcc     4140

aagcugguuu uccagucucc cacagggcuu uuugagaaaa gggugaagcu caccaaucug     4200

uga                                                                   4203


<210>  3291
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3291
augggcaagc agaaauucaa cgacuacuau cugggguuug auaucggaac caauagcgcc       60

gguugggcug ugacagaccu ccaauacaaa cuucugagau ucaacaaaaa ggauaugugg      120

ggcuccaggu uguuugacga ggcacagacu ucuaaagaac ggcgcgucua ucgaaauuca      180

cguagaaggu uagaucggcg caagaagaga cugaaacuac ucgaggaacu guuccuugac      240

gagauuagua agauagauaa cacguuuuuc aaaaggcuga aggaaagcaa uuuguacaac      300

gaggacaaau ccaucaaguu uaauuauccc cucuucaacg auaaggacua caccgauauu      360

gacuuuuaua augaguacaa aacaaucuac caucugcgga aggaucugau uuucgaagac      420

aaaaaguuug acauccgccu cguuuucaua gccuuacacc acauccugaa auaucgaggg      480

cauuuuauuu ucgagggaca gucuuucgaa gagaucaagu cguuugauuc aauuuucacu      540

cagcuuagca guuacuuuuu ggagaacuuc gacuuuaaua ucgauguguc cauaaaagaa      600

aagcuggagg aaauuauccu aucugacaac accaagagca uuaaaaagac agaguucaaa      660

aagaucuuuu ccgauaauaa aguacucuca gugcugaaca uggcgaucgg caguaagguc      720

agccuuaagg acguguucaa uaacgaggaa cugaaagagc aaaagauuuc uuuuuccgaa      780

auagauuaug agaacgaaaa gagagacgag uucuaccagu ugcucgguga agauaucuuu      840

cugauugacu uagcuaagaa aaucuaugau uucaugguuc ugaagaagau acucaaaaau      900

accgacucau uuagcgccag uaagguggca cuuuacugcg agcacaaaaa ggaccuggau      960

gacuugaaau auauuuucaa gaaguaccua acuaaagagg auuacuucaa guuuuucagg     1020

ucuaacgucg aaaagaucaa uuauccuucc uacaucgggc ugaacaagau uaaaagcaag     1080

aaaaucauua aggagaauaa guguucgaua ucugacauca acaaagaacu caagaauaaa     1140

uaucugaagg auauuaaacu ugaggccaag gacaaggaac uguuuaaccg gaucuucaau     1200

aaauuggaug aguuuaaguu ccucgagaaa cugaaguccu cagaaaacag uacagugcca     1260

uaccagguac augagaucga acugaaaaag auucuggaca aucagcguaa guauuacccg     1320

uuucuaaacg agaaaauaga uggaaagagc guuuaugaga aaaucauuca acucuucgaa     1380

uuuaagaucc ccuacuacau uggcccucug aauaccgacc caaacgugaa aggcaagaau     1440

acgugggucg ugcgcaagga ugagaacaac aaaacuccca ucaccccuug gaauuucgaa     1500

cagaaggugg acaugcagaa auccgcugag gaauuuauaa agagaaugac aaacaggugc     1560

acuuaucuua aagcagagga ugucaucgcc aaaaauucuu ugcuguacga ggaguucauu     1620

cuccugaacg aacuuaauaa aguuaccgug gacaacgaaa uccugcaccc aacauugaag     1680

accgagauua ucaaugaacu cuuuaagacg acuguaaaag ugacaaagaa aaaguucaua     1740

gaguuccuga aaaagaagca ggcugucaac gacguggaga gcauuaccgg gaucggaguu     1800

gaauuuaauu guaaauuaca agguuauaac acuuuccgga agauucuggg caaaaagauc     1860

gagaauguga acgucaaaaa uuuuguggaa aagugcauag agcuaaagug ucucuacggg     1920

ucagauaaaa agcuguucga aaacgccauc ggaaaaauuu aucgagagua caauaucaca     1980

gacgaggaau uuaacaagau uaaccgccuu aauuucaaca aaugggcgag auugaguaag     2040

aaguuucuga ccgagaucuc cuucgagaaa acggauggca ccaagagguu ugaaagcgua     2100

auagaggcac uccggucuac aaauuauaac cugauggaau uacugucaga caaauucaau     2160

cuucagagua agauugauga gaucaacggu gaagacgauu uuagcaauau cgacauuaaa     2220

gaguacguug auaaguugua cguguccccc ucugucaagc gcucgaucau ucagacuuau     2280

aaaauagcca uggaaaucaa gaaaauuacc aagaaagacc cuaagaaaau cuucauugag     2340

auggcuaagg auaaggggga gaaaggaaag acaacugaga aaagaaagga caaaaucaag     2400

aagcucuaca aaaacauaaa ggauacccug agcgacuauu gcaauaaccu acaagaccug     2460

acacuccagg uggaaaaaua cgaugacaau aggcugcguc agaagaaacu uuucuuguau     2520

uuuaugcaga ugggcaaguc cauguacacc ggccggugua ucgaucugga agacuuauuc     2580

aacaauaacg ucuaugauau ugaccacauc uacccacaau caaaggugaa agaugacagu     2640

uuugauaaua uuguucucgu ggagaaggaa gagaaccauc gaaaaucuaa cgagcugcuu     2700

aaggaagaga uccagagcag aaugaauaac uacuggauau uccugaggga aaauaaauuu     2760

auuuccaagg agaaguauaa ccgcuugacu cggaaaaagg acuucacgga ugaggaacuc     2820

ggggacuuug uagccagaca gcuggucaca accaggcagu caacuaaaga gaucgcaaag     2880

uucuuugaga aaaucuucga aaagaccaca gugguuuaca gcaaggcuuc ucuagugucc     2940

gaauuucgcg agaaguauga cauuauaaag guccgagagg ugaaugaugc ccaccaugcg     3000

caagacgccu accugaacau cguaguggga aauguuuuca acaccaaauu uacuaagaau     3060

uauuggaaau ucacaaagga uauuaaacgg cagaacgaag agaauccgaa cgaaaagaag     3120

gguuacaauc uccguaaaau cuaugagagu gacgucuucu gcaacgauag cguggcaugg     3180

guguacucua aggaaaaucc caaauacaac acgauuaaua ccgucaagga caugguuuau     3240

agaaaaacug cuaacaucac aaggaugcug guggagggca aggggcagcu uuuuaacuug     3300

accauaaagg auaaaacaca gcugaagaaa aaugugggaa acaucguagc caauacuccu     3360

auuuccuuaa agaaccgcaa acuccacuua aauucagucg agaucgcuaa gaaguacggc     3420

uauuucgaca gucuuaacaa ugccuacuuu gugcuguuca aauaugaaga gaaggguaaa     3480

gaaaagauug gguuugauag caucaccaua gcauacaaag agcggcuaaa gaaaaaccuc     3540

gacauugaaa aguuccugga gaauaagggc uauaaaaacu cgaaguugau cagaaaaauc     3600

uuuaagaauc aaaaaguucu gauuaacgga uacccauacu uacucaaguc cuucaauaag     3660

cagucucaga ucaaauuuaa caauuucaag auacuguauc uuaacgagaa caaucugaaa     3720

uuugccaagg augcuauuaa auucgaaaag aacaaugcga acguggacuu gaagaaacag     3780

aaguacaucg ucagcuuucu caaaaagaaa aagcaaaaug agauuaacga aacguaugag     3840

gaauguaucg auaggcugaa ugacgaguuc gacacccuau uccugaaacu uugcgauaua     3900

cagaaaucaa guauuuacac aaacuauaag cuccaggacu uuuccucuaa uaaaaagagc     3960

aucgagauca aagauaaguu cgacauuuuu aagucccugg auuuguacaa aaagucaaaa     4020

cugaucaagg aguucuuaaa cuauuacaaa uacgaaaaga augaccucga uuuuagccug     4080

auugacauag auaaaaaggc aaacuguauc caaaagcacu ucacugaaga ccuugugaaa     4140

gauuuuguua uuguggacga gaguguaacc ggccuguucg ucaagaaaga gcgauugaag     4200

uga                                                                   4203


<210>  3292
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3292
augaagacca ucacagagga cuacauucug ggccucgaua uagggacuaa cagcugcgga       60

ugggccguga uggacaaaaa gaauagacuu cugagguugc gggguaaacg cgcuaucggc      120

ucccacuuau ucgaagaggg gcauucugca gccgaucgac guucauuuag aacgaccagg      180

cggcgccuga agagaaggaa auggcggcua cgccuccugg aagagauuuu cgcggaaccc      240

auggcuaagg ucgacccugc cuuuuucguu cgacuucacc agaguugggu gagcccacug      300

gauaaggacc ggaaaaagua ucaugcaauc guauuuccga cagccaaaga ggaucacgag      360

uucuaccaug acuaucccac uauuuaccac uugagggaug cucucaugcg ggaagaccgu      420

caauuugauc ugcgcgagau cuuccuggca auacaccaua ucgugaagua uagaggaaac      480

uuucuccagg acaccccugu caauuccuuc guggccucua aaauugaugu ugcuucguuu      540

uuaacaaacg ugaaucaggc cuacgcggac aaguauggcg aggaacagaa cuucaucgag      600

cugaaugaaa acaaugcagc cgcuauugag gccgucauca gggguaagga caaagauaag      660

accacuaaac aacuugacaa ggcgaaaaag auaacaaaau ugcugcaguc aagcaccaac      720

aaguacgaaa agaaugugac gcggcagauu gcuaacgcca ucauugggua caaaacucag      780

uuugagacca uccuaggaaa ggauaucgac aaaaaugaua aggcggacug ggaguucaaa      840

cucagugaug gcgacgccga ugacaagcug gaaaaccuuc ugccagaguu cgaugacaau      900

gaccaauaua uuguagcaga aauaaagaaa uuguuuggcu ccaucgcucu cucucagauu      960

guuuacgaga acaugacacu gagccagucc augaagugua aauauaagga ucacggggac     1020

gauuaccauu uacugaaaca guauaucgcc acucucucag accacggaca agugcgaaaa     1080

cuucuguugg cuuacgaucu auaugucaac aaucgccacg guagacugcu cgaagccaag     1140

aaagaguuca agcaggacaa agcacugacc caggaugaau uuuacaagau agugagggag     1200

aaccuugaca auagucaacu ggcccagaaa auuuugcaga agaucgagga agagaaguuc     1260

augcccaaac agcgggugaa caagaauggc uuuaucccuu accaacucca ucagauugaa     1320

cuggaucaga ucauugcuaa ccagagcaaa uauuacccau ucuuagcgga ggaaaauccc     1380

gucaaggagc accgcaaaca agcaccuuau aagcuggacg agcuaguuag auuuagggug     1440

ccauacuaug uagggccgcu cauaucuccc gaugacaaac guaaggauaa gcagcagaac     1500

aaaaaucaug aaauguucaa guuugccugg augaaacgga aggacaacaa uggagcuauc     1560

acaccuugga acuucgccca gaaaguggac cgagagaccu ccgcaaauag auuuauuaag     1620

aggaugacgg ucaaggauac uuaccugauc ggcgaagacg ugcuuccagc ccgcucauug     1680

cuguaucaaa aauucgaggu ucucaacgag cugaauaaca uccgggugaa cgguaagaga     1740

cuugaaccca aauuuaagca gaaagucuuc aaugagcugu ucaagcagaa gaacaaagug     1800

acaaagaagg cuuuuaagga guucauuuug cagaauaccg ggaugaaaac ugccgaaaua     1860

gauggacucg cagacccuac aaaguuuaac agcagucugu cuuccuacua ccacuuaaag     1920

agcaccaaag uauucgauaa gcaacuggac gauccagaau aucaggcgga cuuugagaaa     1980

aucauugagu acucgucuau cuucgaagau gcugagauuu aucaggaaaa gcuacauaaa     2040

gagaucaagu ggcucauacc cgcccaauuu aaagcaaucg ccaauuggag gcugcagggc     2100

uggggccgcc uuucccgaaa guugcugguu gaacucaagg ggaaaggagg ugagaacauu     2160

aucuaccagc uguggacgac ccagaagaau uucaugcaaa uugugaacga gccggaauua     2220

aagaaggcua ucgucaaaga gaaucaccag gccacagaag agaagggcau agaaaagauu     2280

cuggacgauu cauuuacuag cccugcaaac aaaaaggcua ucagacaggu gauuaaaguc     2340

guggacgaug uugugaaggc cgcaggggga aagaagcccg cccaguucgc uaucgaguuu     2400

acccgugagc caaaguggaa uccucaacuu agccacauaa gaggcacaaa auugcuccag     2460

guauaugaag acacugcaaa gaaacugguc gaccaguccc uaaccgauuc ucugaaggag     2520

aacaugacaa auaggaaccu ccugaaagac gaauacuucc uuuauuuuau ccagugcggu     2580

cgcgaugccu acaccgggaa gaagauugac aucgcugaua uucauaaaaa guaccaaauc     2640

gaccacauau ugccccagag cuucauuacu gaugacucac uggauaaucg gguguuaacg     2700

gagaucgaga ucaaccagaa aaagcauaaa aaguaugcca gucaccucuu cgcaacaaag     2760

ccagcguuug gccugaacca auccauuagc gaaaugugga aaaccuggca gaaggccgga     2820

cuuaucucuc agagaaaacu gaauaaccua cucuucgacc ccgaucuggu uaaggacaaa     2880

cagaagaaag gcuuuauuaa uaggcaauug guggagacuu cacagauaau caagcugguc     2940

aguauuaucc uccaggacaa guucccugau accgaaauua ucacagugaa agcuggguac     3000

aacaccgccc ugcgaaagga gcuuaaauug uauaagagcc gcgaagcaaa ugacuaccac     3060

caugcuauag augccuaucu gacugcaauc aacgacaacu uuuuauacca gguauaucca     3120

aauuuccggc cguuuuucgu guacggacaa uuuaaaaagu ucucccagga uacagacaag     3180

cagaaagagg ccauuaagua cuauggucuc aaaaauuuuc uggagccccu ucuggaugac     3240

ucuacgggcg augaaguuau ggucaaccac accaagcaug ugguguucca caaacagaga     3300

gacaucuuca auaggcuaau ucaggcuuac aacuauaagu acaugcucgu cucgcgugag     3360

acuacaaccg aaaauagcaa cauguuuaau augacaaucu auccucgcgg ggagcgggau     3420

agaaagaaaa cuaggaagcu gauaccaaaa ggaaaggacu uggaucccgc gauuuacggc     3480

gguuauuccg ggaacaccga cgccuacaug guuaucguga aaaucgaaaa gacgaaggag     3540

acccuguaca aagugguagg cgucccuaug cgagcauuag cuucacucaa gaaagcccaa     3600

aaggagggaa acuaugacca ggaacugaau aaagugcuug agccacugau uauguucaac     3660

aagaauggca aaaguaaggc cgggauaaag ggauuuacag uugugaaaga ucauguccac     3720

uucaaacagg ugguuaucga cggugauucu aaauuuaugu uguacagcgc ucaauaucgg     3780

gcaaacgcca agcagcucac ucugucccag gaaaccauga aagugguaac agacaauacu     3840

gaugacaugg aucaaaagaa cgcggacaag gaucagcuac ugcuuaaugc uuacgacgag     3900

auucucgaaa aagucgauca guaucugccc uuguucgaca ucaacggcuu ucgccagggg     3960

cugcacgaug gcagagccaa guucgcagcc uuaaaaaccu cagagaagaa auacacgcuc     4020

aagaauauuc ugaacggacu ucaugacaau cuggugacac cgaacuugaa gaauaucggu     4080

auaaaaaccc cuuuugggcu caugcaaagu ucuuccggca ucacucugag uccaaacgcu     4140

aagcuagugu uccagagccc cacaggacug uuugagaaaa gggucaaacu uaccaaucuc     4200

uga                                                                   4203


<210>  3293
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3293
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc ccuggugaaa       60

gagacugaaa auuccaacga gaagucugaa auaaucaaau ugggugucag aguuaauccc      120

uuaacggugg augagaagac caacuucgaa gcuggcaggc cucugucaac aaaugcagac      180

cggacugcca aacgcagugc gcgacguaac cuacagagau acaagcucag gcggaagaau      240

cugauugauc uucugaucaa acaccgcuug auugacaagg auaccccacu cacagagauc      300

gggaaaaaca ccacucauca aacacuggag cugagagcua aggccgcaag ggaacggaua      360

gagcucgaag acuuagcccg cguauuucug gcuaucaaua aaaagcgagg auauagaagc      420

uccaggaagg ugaacaauga ggaggaaggc caggucgugg augguauggc aguugccaaa      480

aaguuguacg acgagaaccu uaccccgggg caguaugcuu acgaacugcu aucuaaagga      540

aagaaauaug ugcccgauuu cuaccggucg gaccuccagg ccgaguuuga uucaauuugg      600

gaauaucaaa agcaguucua cgcggacauc cuggaugacg agcuuuacaa ugcacugaaa      660

ggccagggcc agcaaaacag ccguaagcgc uuuuuggcca uuaagggggu cuauacggcu      720

gagaauaaag gaaagagaga cgaagugaaa cuccagcacu acaaguggag gagugaggcc      780

aucacucaga aacuguccau agaagaggua gcauauguuu uaguggaaau uaacaacgau      840

cugaauaagu cuagcgguua ccucggcgcu aucuccgacc ggucaaagga gcuuuauuuc      900

aacaaagaga ccgucgggga aaaucugugg aagcagauuc aaaagaaccc ucauacaagu      960

uugaagaauc agguguuuua ccgacaggau uaucuagacg aguucgaaca aaucugggag     1020

acucaggccc aguuucaccc acagcugacc cucgcgcuga aagaacaaau ccgcgaugug     1080

gucauuuucu accagagaaa acuuaagagc cagaaaggac uguugucuuu uugccaguuc     1140

gaguccuggg agauagaaag gaaggacgag aacggcaaug uuauccucaa caaaacaacc     1200

caacugccca agcggcagac ggugggucgc agaguagccc cuaaaucaag cccauuauuu     1260

caggaguuca agauuuggca gaauaucaac aaucuggaga uagcaaagau uagugauggg     1320

aacucuaaaa auaagaaaga gacugaagcu cuauccgacg augagaggaa gcuccuguuc     1380

gaagaacuua acuugcgugg aaaucugagc caaaaagaag ugcuccagau ccugggccuu     1440

aaggacaaag aguacaagac aaacuuuccc gagggccugg aagggaaccg gaccaaugcc     1500

gcuuuguuca acaucuauca gcagauugcc gagaaugaag gauacgguga uuggacuaag     1560

aaaucggcac aagagaucaa ggaggaacuc aaagccgucu uuccucagau uggcauacag     1620

gcugacaucc uggacuucaa cgcggaguua gaugggaagg aauuugagaa ucaagcaucu     1680

uaucagcugu ggcaucuacu cuacuccgcc gaagaggacg auaaaauuaa cgaagaggac     1740

cagaucaucu auggaaauuc agcugugagu cugaagaaga aacuuuguga gaaguucggc     1800

uuuacaccag aauacgccaa auggauugca aacguuagcu ugcaggauga cuaugguaau     1860

cuguccacca aggccaugcg aaaaauaauc ccguaccuca uugaugggaa cgacuacucu     1920

gaggcuugcg cccuggcagg cuauaaucac agcaacucau uaacgaagga agagaaugau     1980

aacagagaga ggcugaauaa acuugaauug cuccccaaaa acagucugcg caacccugug     2040

gucgagaaga uccuaaauca aauggugaac guaguuaauc aggugauuga aaccuacgga     2100

aaaccagacg agguccggau cgaacuggcg agagagcuca agaaauccgc ugaggaaagg     2160

gccgagauga caaagggcau aaacgaagca acucgccgaa augaggauau caagaaacug     2220

auuaccaaga acuucgggau ccccaauccu acaaagaacg acgugguccg guaucgucuu     2280

ugggaagagu uggccccacu ggcuuacaag gauguguuua cuggaagaca gauuaaaaag     2340

gaggacuuau ucagcucuaa aaucgacaua gaacacauua uccccaaggc ccuccuguuu     2400

gaugacucau ucaguaauaa gacccuugca uuuagggaga caaaccugaa aaaggcugau     2460

cgcaccgccu augguuucau ugaaagcgac uacaaugcga cuuuggauga cuauauccag     2520

cggguugaga cgcucuacaa caaugccaaa ggcacaauau ccaaggggaa aagaaacaag     2580

cugcuaaugg cucaaaagaa ucugccggau ggauucaucg aaagggaccu ccgaaacucu     2640

caguacauug ccaaaaaggc aaaaucgaug aucgagaagg uguuuaacga uguaaauguc     2700

accagcggcu ccauuacuga ccgccugcgg gaggauuggg accuuaucaa cgugaugaaa     2760

gaauugaauu ucccuaagua uaaggcucug gguuuaaccg agauagaaga gagauacgau     2820

auugggcagg agaaaacaaa gaaaguugaa gugaucaccg acuggacuaa gaggaacgac     2880

caucgucacc augccaugga ugcacucaca gucgcguuua cgaccaaauc acacgugcag     2940

uaucugaaua accuuaauac uauccacagu cugaagggcg acgagguaga ucuagaacuc     3000

ucuaagcugu acggauugaa aaacacaauu accgaggugg uuaauaagaa acgcaaguuc     3060

auucccccaa ugccuaacuu ucgggaagag gccaaaaagc auaucgagac aauacugguc     3120

agcaucaaga auaaaaacaa gguguauacu aaaaauauua acaagaccaa aaaggaaucc     3180

ggcuaccaca cgaaagagca auuaaccccc agagggcagc uccauaagga aacaguguau     3240

ggaaagucaa aaaggccaau gaauaagccc acuaaaauca acaagaauuu cagccuggag     3300

caggcucaac uuauuaucaa cguccaggaa aaagagcugg uuuugcgaca ccucgcccag     3360

uuugacaaca auccugcagu ggcuuucgau accaagacac ugaagaaaau gccacuacug     3420

aagaacggug agccgcuuaa agaagugcuc uguuuugagg aaauauucac uauccggaag     3480

gacauuaccc ccgaucugaa aaucgagaag guagucgacg aaaagauuaa agccaucuug     3540

gagcagcgca agaaagauuu uggcgggaau gcaaaggagg ccuucucuga ccuggauaaa     3600

aacccuauau gguuaaauaa ggaaaaggga auuugcauca aaagagugac gaucacaggc     3660

guuuccaacg cugaggcgcu ccaucaccaa aaggaccacc uggguaggga aauucuugac     3720

gauaauggga acccaauacc cgccgacuuu gugaguaccg gcaauaacca ucacgucgca     3780

aucuacgagg augagaaagg aaagcugcag gaaaaagugg uuagcuugua cgaggcugug     3840

gcccgggcca aucagggccu cccuguaguc gacaaggggc ugaacgaaga ucuaggaugg     3900

aaauuccugu ucacuaugaa gcagaaugag auguuugugu ucccaaacga cgauuuugac     3960

gugaaugaua uugaccuucu cgaugaaaac aacgcuaaga ucauuucuca acaucuguuc     4020

cgaguccaga aauuggcaac aaagaauuau auguuuagac accacuuaga gaccaacguu     4080

caggagguga aagaacugaa ugacaucgcc uacaagucca uacgcucaac ugagccccuc     4140

aaaaacauca agaagguaag gauuaaucau aucggcaaaa uugugcaggu cggugaauau     4200

uga                                                                   4203


<210>  3294
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3294
auggccaaga aagacuacac caucggccug gauauuggga caaacagcgu gggaugggcu       60

auaaucgacg auaaucucaa acuucugaaa agaaacauga cuauuaaggg uaauacggac      120

aaaaaguccg ucaagaggga uuuguggggc ucuuuacugu auucagggaa cagugacaaa      180

accacaagcg cagccgaugc gcgguccaag cgcggacuac gacguagacu caggcggcgc      240

aaauacagac uggacaggcu uaagcagauc uucucugaga uuaucaauga uaaagcuccc      300

aacuuuuucg acaagcugaa ugaaucguuu uugaacccua aggauaaaaa guauggcaaa      360

uaccaaauau ucgacacuga gaaggaagag aaagauuauu accggcgcua uccaaccauc      420

uaccaccucc gaaaggaccu gauugaauca agcaaaaagc aggacaucag acugguuuac      480

cucgccuuag cacauauucu gaagaguagg gguaauuuuc uuuucgaggg gaacaucgau      540

gacuugaaaa augauuuugc cggaauauau gaggaagugg uagagcugug caugacaauu      600

aacgcugaag acguggaucu agaguucgag gaagucgaca agcagucccu caauucuauc      660

auuaaaaacg aggauaucag cgaaaucgag cagggccugg aaaauuuugc agacgagcac      720

gugauuuuca aggagcaaaa caaaaagaag aacgaucugu uuuccaauug uugcaaaaua      780

aucuguggcc auaccguuaa ggccaacaaa uucgcuucag aacuugacag ugaguuguuu      840

auuagcuuca agucugauga cuacguggac gucaucgaug ugauacaguc cgggaaugaa      900

aacauugcca aucuccuguu agcgugccgg aaagcauaug acuacaucau guucaaccgu      960

cugguagauc ucaauaucga cucaccggcc aaacuuagca guaacauggu uucucuguau     1020

aaucagcacg agaaggauuu gaaggcuuac aagaaacuaa uuaaggaauu uaacaaauuc     1080

aagcgcucca auggauguaa ggaccuggag augaucauuc ucacugccga ugacauagau     1140

agcuuuagaa agaaggugga caaaaaggag gguaaacuga acggcaucaa uaagaaaauu     1200

acacaugaac aggcacuuaa gaagcaacug aaagauauga agaaaaucuu ggaggacaag     1260

aacaccgaag cugaggauaa acagaucaau gacauucuca agaugauaac gucgaucgaa     1320

gagaggguca acaagucuug cuuccugaaa aacuuacggu ccacugacaa ugccucaauu     1380

cccaaccaga uccagcgaca agagauggaa gcgauucugg auaagcaggc caaauuuuau     1440

ccuuuccuaa augagcacaa ggacgaacuc cugcagcuuu ugaguuuucg caucccauac     1500

uacguggggc cccuggugaa caaaaaguau agcagauucg cauggcucgu caggaaggag     1560

ggacaaguuc agaaaauaac cccuacaaau uuugauggcg ugguagacaa gcacaaaacu     1620

gcugagaagu ucauggaacg gcugaucggu aaagaugugu accuuccaaa cgagcgcguc     1680

cugcccaagg ccuccuugcu cuaucaggaa uacuguauuu uuaaugagcu gaccaaggug     1740

gcuuauaucg acucuacagg gaaaaagaac aauuucagcu cagaagagaa auuaaacauu     1800

uuugagaagc uguucaaaac caagagagaa guuacgaaaa cugaucuaug caagugucuc     1860

aauaacgugu gcaagcugaa agagaagguc aaagaaacag agaucauagg aauuaaggcc     1920

aaauucaaug caaaguacag uaccuaucau gaccuuaaga aaaucaacgg cauggaacag     1980

uugauugccg augaggaggg caagccucug ugugaagaca ucauauccau ccucacuauu     2040

uuugaggaua aagacaucag gcuggugcgu uuaaaggaac ugcuuugcca aaauaaagau     2100

uugauuaaca aguucagccu cucugcugag aagcuggcga aaguacuauc aacaaagcac     2160

uacaaagggu uuggaaacgu uagugcaaag cugaucaaug guauacggga caaaaacugc     2220

aagaccauuc ucgacuaccu gaucgaagau gacaaggagg ccuauuacgg ccgaaauaac     2280

ccaaauagaa accuuaugca guuggugaau gauagcaggc uggcuuucaa agggcagauc     2340

gaccgcgagc agaacacgca uuuagaagau cucucccugg acgaguuucu ugaugaccug     2400

uaugucucuc cgucgauucg gagaggaauc agguugacca uucgccucgu ggaugaacug     2460

gucgagauaa ugggcuaccu acccaagaau aucgugauug agaugccucg agaagacggu     2520

gagaaaggga agaucgccga uacacgguau agcaaacugg aaaagaugcu caagaaagac     2580

gcagcccugg aggaccuuua ccguguuuug aagacuuaug agaaaaacaa gaaagcucug     2640

gccaaugaug cauuauaccu cuacuuccug caaaacggca gagacaugua uaccggaaag     2700

gaaauuaauc uuuccgagcu gcacucauac gauaucgacc acauaauccc aaaaaguuuu     2760

aaguaugaug acucucuaga uaacaaggug cucacagcga aaaagaugaa uauggacaaa     2820

aggacuggcg cucuggauca uaacauuauc gaaaaccagu guggguucug gcgcguauug     2880

cugcagcagg acaagauuag cuuagagaaa uacaccaauc ucaugaagac agaauuuacc     2940

gaggccgaua aggcaggauu caucaugcgg caacuggucg aaacuagaca gauaacgaaa     3000

uuuguggcca gguaucuuga caacaaguuc aauggucuga uuuccgaucc caacgacaaa     3060

guuaauaucu ugcucccucg agcuucacug ugccaccagu uucgcgagac auucggcuuc     3120

uacaaggugc gggagcuaaa cgacaugcau cacgcccaug augcauaucu gaaugcuguc     3180

aucgccaaca cccuuaauaa aaacgcguac cucagcgacc uguugaagua cggggccuau     3240

ucuaaguaca aaaagaaugg auuuaacaau uccaacggca uuauggauua uuucgguaau     3300

acucaguuua acugucuguu cgugguagaa agaaccuuag acaaaugcag ggugaauaua     3360

guuaagcacc cagagacagc uaguggggaa uuuuacaacg agaccaucca gaaaaacaag     3420

gucaauggcg gaagcucuac ucguucccuc aagucaagug ugaaagugcu gcaaaacaca     3480

gaacaguaug gcggguucac gaaugucaac aaugcauacu uuauucuuuu cgauuauaag     3540

gccaaaagca agcugaaacg caaguugauc ggaguuccca uuguggaccg gcaaaaauuu     3600

gagcaggauc cggugaccua ccucgaggcu aaggguuucg acgaaccuaa gcugguacag     3660

aaacuacuga aguacacucu ucucgaguau gaagauggca aaagaaggua ccugacaggg     3720

gucaccggaa agcgauguga guuggugcgg gccaaccagu uacugcuccc ccgcaauaug     3780

auggcacugc uucaccaucu gcaagagugg cagaaacacg acuuuggcau caaggaaaug     3840

acaaagguua ucaaaaacac uaauaacaua gaggcgaagu ucgauaaauu guucgaacau     3900

augaugaagu uuauugacaa auauucggag ccaccuaaga ucguguccuc uaagauuagc     3960

gaagaguacc acaaacuaag agagucacug ugccaggaug acaauaagau caaaauauau     4020

gccgaaauug guaaggcucu ccugagucuu uuacaccugg ucgacuccaa aucugccugu     4080

guguucaagu uuagcgggcu cgagaucaac aggauccggu accaguccau uaaugaaaag     4140

aaagagcccg uuaucauauu ccaaucauug agcggccugc gagaaaguag auauaaguac     4200

aaccaguga                                                             4209


<210>  3295
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3295
augaagcuga gaaacgccaa agacuacagc aucggccucg auguggggac cggauccaca       60

gguugggcug ucacuacgcu ugacggcgau cuguucaccu uuaaucacag gcccacauug      120

gggucucggu uauucgaguc agcaaguacu gcccaggaaa cccgcgaggc gcgaggagcu      180

cguagaaggc ugaagcggcg cagauggagg auuagccuac uccaagaaca guuugccgca      240

gagauggaau ccugcgacuc uaacuucuuu auacggcuga aucagucgcg ccuuuggcag      300

gaggaucgaa cagagcaagg caaagguuau cuguucgggg gaaagccuau cuuguguggc      360

gcccagauuu uugcuaacga acaggaguac uaucagcaau ucccaaccau cuuucaucuc      420

agaaaauucc ugguugaaug cucagaacag guggaccuga ggcucauuua cuuagcacug      480

cacaauaucg uaaaguaucg gggcaacuuu cuuuaccagg auaauaagac uuugaacgug      540

cguaauuuca accuggacag ccagauagcc gauuuucuag accaacucca gcuguggugu      600

gagaauaacg aucuuugcuu ccaugaaauc gacgggacaa ccacgcuggc uagucaguug      660

aauacuuguu uccaccaaca guccaaccag cagaaaaagg auacccucaa aacauuuuuc      720

gccacuugca uugagaauga caagaucgcg ggagaacuga ccaaagcauu acuggguuau      780

aagacagacu uuaagauuau cuucaacaua gagggcgaaa aagaugagua caaggccaaa      840

uguaacgagg aagagaagau ucucaaacuu caagaaacca ucgacgaugc uaaucagguc      900

cuguugcagu cuauucuaaa ccuguacagc gggaucaucg uggagaagau ucucaauaac      960

acggccguua aaaaugaauc cgcacagugc acucugucag cuuaucauug uuacaguuau     1020

gcccuuuaca gcaagcaacu gcgcacauug aagaaacucg ugagaaagua uaacccgcag     1080

agguacaaug agugcuuuaa ccugucugcg gaggacccca aagccaagaa acaguuaugu     1140

accuaugcac gguacaaugc ucagcacucc acugauuacg acgaguucaa gaaguauaua     1200

aaagaucugu uugagaacac agacgccguc aaugaugcug ccuaccaaca gaugaagcag     1260

gacuucgaug aagagaaauu ucuacgacgc cagaagaccu cagacaacgg aguguauccu     1320

uaccaauucc accucgaaga gaugcagcug aucauugaga aucaggcaca acauuaucca     1380

uuucuuacga aaaucaagcc caaguuggaa agccugguaa ccuucagaau accuuacuau     1440

guuggcccac ucacacagaa agguauucag aggaacgggc ggaaguuugc cuggaguaaa     1500

cgcaauaagg acgcugagaa ccagagaauc uaccccugga acugggauuc uaucauugac     1560

aaauccgcga gcgcagaagc cuucauccug aggaugacug gaaagugcac cuaccuuuau     1620

ggcgaggaug ugcugccuaa ggagucguug cucuaccaag aguucugcgu ccugaaugaa     1680

uuaaacggcg cucguuggaa uugugacggg gaacggcgaa ugccacuguc uaacaaagau     1740

ugcaaggcca uuuauagaca gcuauuucuc aaaaagcaga aaguguccua cgcagacgug     1800

ucaaauuauc ugaaggccca gggacacaua aacaauagug ucgcuggugg ccaaggggag     1860

cauggauucg aaagcacacu uuccucucac cacuuuuuca ggaaauuguu uaacguugau     1920

gccgcaaaug accugacugc gcagcagauc gagaccgcug agcagcucau ucuguggggc     1980

acaaucuucg aagauaagga gaucuuugaa caaaagauua aagagcgcuu cggugacaug     2040

uuuagcaagg augaaauaaa aaccaucgcc cggagaaagu ucucagggug gggcaaaugu     2100

aguaagcagu uacuguccgg acuuacugug gagacgccga ggggccgcga gagcauuauc     2160

gacguauugc gaaacgggca ucccgaaaau ggacggacac ucguguuuau gcaggucauu     2220

accgauaaga aacuggaguu cgacaagaaa aucgaagagu acaacaauga acaccuagca     2280

aagacuaacg agucugugcu ggacaccgcc auaaugaguc cugcuaucaa acgugguguu     2340

aaucaagccc uccauauugu gggcgagauc agaaggauua caaagcacga uccaaccugc     2400

aucuauauag aaacuacacg cgacgagaag gaugggaaac auaacacgcg gaccucaagc     2460

agauacuccu cucugaagaa aaaccuugaa acuuugaagu cgauugacaa agcaaaugcu     2520

cccgccauca agcacagccu ggaguuacuc gagacauaca agaaacagaa cauugaugac     2580

aagcuguuuc uuuauuucac ccagaaugga aaauguaugu acuccggcaa gucacuggau     2640

aucaacaauu ugaguucuua ucagauagac cacgucaucc cucaaagcua cauuacagau     2700

aacuccuucg acaauagggu gcucguauau gcgucagaaa accagcgaaa aagcgauucu     2760

cugcuacugu ccaguguuau ccaggccaag caggagccau uuuggacuag ccucgaaaaa     2820

gcuggucuga uuucugagaa gaagcauaau aaccuuaaac gcaaguccuu gucagacgca     2880

caaaucaaag gguucauaaa ucggcagcug guggaaacca gucagauugu caagguguua     2940

agagaugagc ucagcaggau ccugcccgac acgaaaauca uuccggucaa ggccucgcuu     3000

ucccacgacg ugcguaucgc uugcaaccug uacaagucuc gcaccgccaa uagcuuucau     3060

cacgcacacg augcgcuacu cgccucaaca augggccggu ucguucagac uagauuuccu     3120

gacauguaug agaaccccgu gagguacgua aaaauucugc gagcuuacau agcccaaagu     3180

aaguugcagc auuccgcaca gacccggaau uucgauucuc gcagcuuuau ggcucacucc     3240

uucuuuugug acuauucaaa cgaaaacgga gauacaaucu ggaauagcgc cagugagauu     3300

gaaagacuga ggcgggcauu aucuuacaaa aaccucuauc ugacucgaag accauucauc     3360

gacgccggcc aguuuuggga ucaaaccauu uacuccccuc augacaagaa aaagacgccc     3420

gauagccuua ucccacugaa gaaaaauuug gacacacaga aguauggggg auacucgggu     3480

aaagauuaug cucacuucuu cauauacuca guccucaaca agcagggcga cguggaguac     3540

cgcuuugacg ggguuccccu guauaucgcg gccaauauua acagggauga ccguggagca     3600

cuagcucaau acgccaaaca gcugguggaa caugagaaug ucccuaacgc caagcggaag     3660

accaaagauc uuguguucca gagaaucgag agggcuugcg uaccaaagua ucagcucauu     3720

uguaucucuc aaugcagugg cgacuccgau cgcagcgugu cugacuacua uuacauaacu     3780

gguaaaaagg aaguucgaaa ugcacugcag uuggccuuuc cgaugcaccu guauggggcg     3840

auuaacgcua ucgaggauaa aaaggaauua gacgauagcg cccucgagga acuggucgug     3900

uacauccuua gaaaugcacc ccggauugcc ccugacgugu ucaggcgccu gaacuugaca     3960

gagaagcucg cugauugucu ggacauacca gaucguaucg agcuacaaaa uuccauuuac     4020

ggccuguuua accuuaucaa ugcagaauca aacgucguua aucuccagau uuucggaggc     4080

gccaaaaccg cgggggugaa gcugaucaac uugaacaguu uacugaauga ccccgcuacu     4140

accgugacau uuauagacca gagcguaacg ggaauguucg agagaaaaga aaccaucggc     4200

auuucuuga                                                             4209


<210>  3296
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3296
augggcaagc aggaguucaa cgacuacuau cugggguuug auaucggaac caauagcgug       60

gguugggccg ucacagacca acaguacaaa cuccuuagau ucaacaagaa agauaugugg      120

ggcuccaggc uguuugaaga ggcuaagacu gcaaaagaac ggcgcguuua ucgaaauucu      180

cguagaaggu uggaccggcg caagagaagg uuaaagcugc uagaggaacu cuuccuggau      240

gagauucuua aaguggacuc aacguuuuuc aagcggcugg aggaaaguaa cuuguacaau      300

gaggauaaaa gcauaaaguu uaacuauccc cucuucaaug acaaagauua caccgacauc      360

gauuuuuaua aggaauacaa gacaauuuac caucugcgca aagcccuggu auucgaggac      420

cgaaaguuug auaucagacu cuuauucauu gcgcugcacc acauccuuaa auauaggggg      480

cauuuuauau ucgagggaca guccuucgaa gacaucaagu cuuuugacua cauuuucacu      540

gagauccagg auuggauuuu ggaaaacuuu gagaucaaua uagacauuaa ccugaaagaa      600

aagcuagagg agaucauucu cucgaauaac accaaaucaa ucaagaagau cgaguucaaa      660

aagauuuuua gcgauaaucg gauacuggcu auccuuaaca ugggcguggg uugcaaaguc      720

gaccugaagg auguguucaa uaacgaggaa uugaaagacc aaaagauuag uuuuacagag      780

aucgauuaug aaaacgagaa gcgugacgag uucuaccagc uccuggggga aggaauauuu      840

auuguugauu uagccaaaaa gaucuaugac uucauggugc ugaaaaagau ccucaaaaau      900

uccgauuacu uuucugagag caaggucgca cuuuaugagg aacacaagga agaccugaac      960

cgcuugaaau acauuuucaa gaaauaccua aaguccucag acuauuacaa auuuuucaga     1020

aguaauagcg agaagaccaa cuauccuucu uacaucggcc ugaauaagau uaaaacuaag     1080

aggcucauag agaacaauaa auguuccauc ucagauauua acaaggaacu gaaaaauaag     1140

uaucuuaaag acauccaggu ggaggccgaa gaugaggagc uguucaaccg gaucuugcau     1200

aagauuaaug aaaacaaguu ucucgagaaa cuaaagagca gugaaaauuc uacaguacca     1260

uaccagguuc acgagauaga auuaaagaag auccuggaca accaacgaaa auaucacucc     1320

uuccuaaaug aaaagagcga uggcaugacc cucugcgaga agauuaucaa acuguuugag     1380

uuccgcauuc cguacuacgu ggggccccuu aacacgaacc cuaauaugaa caagaaaaau     1440

aagaacacuu gggucgugaa aaaggaagaa aauucgaagu cuuccaucac cccauggaac     1500

uuugagaaaa agguggacga acagaaauca gcugaggagu ucauaaagag aaugacaaau     1560

agguguacuu auuugcgggg agaagauguc auccccaaaa acagucugcu cuacgaggaa     1620

uuuguucugc uuaaugagcu gaacaaggug accguagaca augaauugcu ccauccuaca     1680

cugaagaacg agauuaucga ugaguuauuc aaaaccacgg ugaaggucac uaaaaagaaa     1740

auuacagaau uucugaagaa aaagcaggca aucagcgacg uggaguccau aaccgguauu     1800

gauguugagu ucaauggcaa gcuacagggg uauaacacuu uucgcaaaau ccucggagaa     1860

aagauugaga aucugaacau caaaaaguuc guggaaagau gcauagagcu uaaauguuug     1920

uacggcucug acaagaagcu guuuaaaaac gccgucggua agcucuauaa agaauacaau     1980

gugacagaug aggaguucac caagaucaac aaacugaauu ucaacaagug ggggagguua     2040

agcaaggaau uucugacggg cauucguuuc gagaaaaccg acggaacaga acgguuugag     2100

ucaguaaucg cugcccuucg aaguacuaau uauaacuuga uggaacuccu guccgacaag     2160

uucaaucuac aaagcaaaau ugaugagauc aacggcgaag acgauuuuuc uaauauagac     2220

auuaaggagu acguuaacga gcuguacgug ucaccaagug ucaaaagaag caucauccag     2280

accuauaaga uugcgaugga aaucaagaaa auuacaggga agaaucccaa aaagguguuc     2340

auagagaugg caaaaggagg uggcgaaaag gggaagagga cugagcgccg gaaagauaag     2400

aucaaagaac ucuacaagaa caugaaagac ucccugucug auuauugcaa uaaccuucag     2460

gacuugucga gccaagucga gaaguacgau gacaauagac ugaggcagaa aaaguuauuu     2520

cucuauuuca ugcagauggg aaaguccaug uacaccggca acgauauuga ccugucagag     2580

cuuuuuaaca auaacaucua ugauauugac cacaucuacc cucagaguaa agugaaggac     2640

gauucuuucg acaauauagu ucugguggaa aaacaagaga accauagcaa guccaaugaa     2700

uugcucaaac cagagaucca gucaaagaug aacaauuacu ggauuuuucu gcgcgagaac     2760

aaguucauca gcaaagaaaa guauaaucga cuaacacgga aaaagaacuu caccgaugag     2820

gaacugggug acuuuguagc ccgucagcuc gucacuacga gacagucuac aaaagaggug     2880

gcuaagcugu ucgaaaagau uuuugauggg accgagguug uguacuccaa agccagucuu     2940

gucagcgacu ucagggagcg cuuugauauc auaaaggugc gggaaguaaa ugacgcacac     3000

cacgcucaag augccuauuu gaacauugug guuggcaaug ucuucaacac uaaauuuacc     3060

aagaauuacu ggaaauucau caaggagaac aauagaaaga acgaagagaa cccgaauaaa     3120

aaggaaggau auaacuuuaa aaauaucuac gagucugacg uguucaggaa cgaggaugug     3180

gcgugggucu uuuccaagga aaauaaagag auuaacacaa uaaagaccgu uaauaaaaug     3240

guguaucgaa agacugugaa caucacagaa augcugguag agggcaaggg gcaguuauuc     3300

aaucucacga uuaaagaccg cacccagcug aagaaaaagg ucggaaacau cguggccaau     3360

auucccaaau cacuuaagaa ccggaauacu aagcugaaaa gugacgaacu agcaaagaaa     3420

uacgguuacu uugauagccu caacaauucg uauuucguuc uguucaagua cguggagaaa     3480

gagaaggaaa agaucggcuu ugacgggaua acaaucgucg auaaagagaa guugaaaaag     3540

aacuccgaca uugaaagauu ccuggagaac aaaggaaaua agaacgcuaa guuaaucagg     3600

aaaauuuuua agaaucagaa agugcucauc gauggcuauc cuuaccugcu uaagucuugu     3660

aacaaagaca aggccauacg uuucgauaau uuuaaaagcc uguauuugga cgagaacgau     3720

cucaaguuca cccgcgacgc uauuaaguuu gagaaaaaua acgccgauau cgaccugaag     3780

aaacaaaagu acaucauuuc auuccugaaa aagaagaaac agaaugaaau caacgaaaca     3840

uaugaggaau gcauagagcg gcugaauaac gaguuugaua cucuuuuccu caaucugugu     3900

gacauucaga agaguccaau cuacaaaaac uacaaguugc ccgacuuuau uuccaaaaag     3960

aauguugugg aaguaaagga uaaauucgag gucuuuaaga accuggaauu auauaacaaa     4020

ucuaagcuca ucaaaaauuu ccugaacuac uucaaguaug acaauaacaa acuugaucug     4080

auagcaauug guaucgacag caaagccaau ugcauccaga agcauuuuac cgaggauuug     4140

auuaaagacu ucgugaucgu ggaugaaucc gucacggggc ucuuugcuaa gaaagagaga     4200

cuggaguga                                                             4209


<210>  3297
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3297
augaccuaca gaaacuauca caucggccug gacauuggga caagcuccgu gggauucgcc       60

gucagguacg auaaugguga gcucguucau aagaaaggcu cuaaccuugu gggggcucgg      120

cuguuuaagg aaggagagac ugcagaagaa cgccgauuga aucguggcgc cagaaggcgg      180

uauaaccgca gaaaauggag guuaaaucug cuaaaccaga uauucaagcc ccucaucgaa      240

guaaaugacc cuacguuuuu caaacggcug gagcaaucaa accuugugaa uuacgauaag      300

agugagagcu uuucccuguu gaacaaucag ucugacgaua agcuccacca gcaguuccca      360

accauuuauc aucugcgcga cuaccugaug aaaucgacag aaaaggcgga uauccgacuc      420

auuuauuuag cuguccacca uaucgugaaa uacagaggua acuuucugga cgccucaccg      480

guugauagcu ucgaggcaag uaaucuuaag guggacgaaa uauugacuca aaucaaccug      540

cuacucgaua aucugaccga cgaucuuagg cugaacacac aguacgucac cgacauugag      600

cagacuuugc ucuccaacac agagucuaau aaagacaagg aaaaggugau ccagaaacug      660

uuacugcaca aggaggccau uaaaaucgau aaggaccgga ccgcucaaau agcaaaacuc      720

guacuugggu auaaggccga uauugcuaaa cuguuuggaa ucacggaaaa guugcagacu      780

cccaagcuag agcugagcgc cgcgaacucc gacgaugcac ucauggaacu gucagccgag      840

cuuaaugagg accagauugg cauccuguug cagcucaaaa cccuguacag ucguguucgc      900

uuaaaccaaa ucauuccuag cggcacaucu cuguccaagu caaugauaga aaaauaugag      960

cuacaccaua cucagcucaa gagcuucaaa gaaaauaucc ugcuuaaguu gagaaggaac     1020

accgcugugc ggcgaaaucu ggagcucgcc uacgcacugu augucgggaa cgcucagaca     1080

aagcaagaag uguuugagcc caaaaaggcc cgcuucacca gugaugacuu ugcgucucuu     1140

aaagccaaug caaaguccgc uagcucggug aaagaggccc caaagaacac gaagacuaaa     1200

cugacaaccg augacuucua caauauuuug acugaacucu ucgcuacauu ugagucugaa     1260

cuguccgccg augaggacau gaucaaggaa gucgagcaga uuaaaaaggc aauaaccgga     1320

gacaacuauu uaccuaaaca gagaacggau cagaauggua acaucccaca ccaacugcau     1380

cagaucgagc uaaggcagau ucuccagaau caacugcccc acuacccuga aauggacaag     1440

aucaacgagc agaagccaau ugaaaaacuu guuaccuucc gggugccgua cuauauaggc     1500

cccuugauca caccugagga ucagcagaag ucaagugccg cuaacuuugc guggaugaaa     1560

cgcaaggagc aaggggcaau uacuccaugg aauuucgacg auaaaguaga cagaaugcag     1620

agcgccacca acuuuaucaa gaggaugaca gugaaagaua cuuaccugau caaugaagac     1680

guccuccccg aucguucccu guuauaucag cgguucaagg ugcugaacga gcuuaauuug     1740

guuaagguga acaaucagcu ccugucuauu ggagaaaaac aagcuauaua ccacgaccua     1800

uuugagaagc agaaaaccgu cagcacaaag aaacugcagu cauaucucca aagugaacag     1860

ggcuacggug auaacaucga gauuuccggg cugagcaauc cugacaaguu caacaaugga     1920

cuuaccacuu auaucgauuu uaagaacauu uucggcgacu ugguggagga cacgacaaaa     1980

cagaaggaau uugagcagau cauagaaugg ucuaccguau ucgaggaucg agccaucuuu     2040

aaagcaaagc ugcaagaagu uaauugguua acugaggagc agauuaaagc ccucguggcu     2100

aagagauacu caggcugggg gaggcugagu uaccgccuuc uggccgaauu ggucaacgac     2160

aauggagagc ggaucauuga uauccucugg aacaacaagg guagcaaucu gauguccaua     2220

gugucucagg acgaguucca gcaacagauu gcaagaauca acucgcauga gguccaggaa     2280

cuaggcaugg agagcgugcu ggcgaaugcu uauaccuccc cacagaacaa aaaggccauu     2340

aggcaaguua ugaaaguggu agaugacauc cagcucgcaa ugaaugggca ggcccccaca     2400

ucaauaagua ucgaguucac ccgcaagccg gaagagaacu cugaaauuag caaaucccga     2460

uacgagacua ucacaucaau uuaucaaagc cugucuaagg auaucgagaa ggaagucaaa     2520

gacaaucuua acaagaauaa auccaguuug aagaaaaacc ugagcgauaa guacuauuua     2580

uacuuuacgc agcucggacg ggacauauau accaaucagc cuauucccuu caacgagauc     2640

ccauacuacg auaucgacca cauugugccu cguucuguug aaaugaauga uucccugaac     2700

aauagagugc uugucgcuaa aucaaucaac cagauuaaga agaacaaugc ccccauagac     2760

aguagccuga aaaucacuuc ggagacaagg caacuaugga agaaacuccu ggacuccggc     2820

uugauuacca agcagaaaua uuacaagcug ucuagcucaa ucaagaacac agugagucca     2880

uauguaaaac aggguuuuau uaaucgccag uuaguggaaa cuucccaagu uaucaagcuc     2940

gucgcaaaca uacuggggac caaauaccag aaugaugaca cggagaucau ugaggugaag     3000

gcucaucuua acucugaaau ccggaaaacc uucgaguuuu auaagcugag agaauugaau     3060

gauuaccacc auggcuucga cgccuaucuc acaacucugg ugggaggcua ccuauacaac     3120

agguauccca agcugcgaag cuacuuuguc uauggggauc acaaacgccu ugacaaggcg     3180

cagcucuccg agcugaaaaa uuucaacuuu uugcacggac ugaccaaucc uaagucaggu     3240

agcgauggcu uaauuacagc cagugggucu cagcaaguug ugcucgaccg gaacgaaacu     3300

aucaaaagac ugaauaagau auacagguau aaguacaugc uuauuucccg ugagguguuc     3360

accaaacagg gacaguuuua uaacgagacg aucuacccag cuaagccgaa uacagcaaaa     3420

augauccccc ugaaggauaa caaaccuagc gaaguauacg gcgguuauuc gucaucuaau     3480

gucaguaaga uugccuugau aaaggagaaa aacaccgaca acuaccaguu caucgggauu     3540

ccaauccgcu ccauugcuaa gcucaaacaa gccaaggcaa auagcaaaac uguguaucag     3600

acagaacuga accggguuau caagucugug cuagcgagcc agaaaaccaa gaagcaaaaa     3660

gaguuugaag ucauacugga uaaugugaag uacaaccagg uugugguaga ccagggcgag     3720

aaauauuacc uuggaucccc cuauuacaag aaaaaugcca agcagcucgu caucucaccu     3780

gagaguguga agauucuggc ugacaaagcc acugcagauu ugccagaaca agaggcucau     3840

aacaaguuaa ugaccgugua ccaggaaauc gucgacguuc ugaauaaaca cuucgcccuc     3900

uaugauauua acaaugugag aaagaaacug aacgacggcc uuaucaaguu cagccagcug     3960

cccaauuacg augagacauc uaagaacagg uuugacauau ccaaaucaau uaagacgaaa     4020

uaugcaguaa ucaugcgaau cuugaguggg auuaagaauc agaccagcga ggugccggaa     4080

cuaggacuga aauccccucu caacaagaug caaacuaagg guggcaucaa aauaucucca     4140

gaggccaaga uuuacuauuc gagccccaca gggaucuucu cacauuuuaa acaggucaag     4200

aaucuguga                                                             4209


<210>  3298
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3298
auggagagaa uccugggccu cgaccuuggg gugagcucca uuggaugggc ccuggucgaa       60

gaggauguua agaaccccga aaauaacaaa auaaucaagu ugggugugag gguaaauccu      120

uuaaccauug acgagaaaac aaacuucgaa aagggcaaau cuaucacuac gaaugcuggg      180

cggaccucag cacgcagugc ccgacguaac cugcagagau uuaagcuaag gcggaagaaa      240

cuccuggaga uucuuaucca cuacaagaua aucaaaaaug augcggugcu ggcugagaca      300

ggaaagaaca cuaccuucca aacacaguau uugcgcgcca aagcagccag agaaaagauu      360

gagcucgacg aacuggcuag gguccugcuc uuaaucaaua agaaacgggg cuacaagagc      420

ucccgcaaag ugcagaacga ugacgauggu aaggcaauug acgggcuguc uguugccaaa      480

gagcuuuaug agaagaauua caccccagga caguaugcuu acgauuugcu gaaacgaggc      540

aagaagcaaa ucccggacuu uuauagaucg gaucuacaga acgaacucga caggauaugg      600

gaguuccaga aacaguacca ucccgauauu cugacugacg aauuuaagaa agagcuugaa      660

ggcaaggggc aacgggccac agcggcaauc uuccugaauc guuacaaaau uuauaccgcc      720

gacaacaagg gaacgcgcga ggagaagaaa auccaggcuu accaguggag aguggaagcc      780

uugucacagc cucucgaugu caaggaggug gcauauguaa ucgcugaaau uaauaacaac      840

cugaauaaca gcagugguua cuuaggcgcc auauccgaca ggucuaaaaa gcuguauuuu      900

aaugggcaaa cuaucggaga guaccucuuc gaggagcuua gcgcgaaccc acacacccgg      960

cugaaaaauc agguguuuua ucgacaggau uaccaagacg aguucgaaaa gauuuggaag     1020

auccagacaa aauuucaucc cgaguugacu aaggaacuaa aagaacaggu ucgcgauaua     1080

gucauuuucu accagagaaa gcugaaaucc caaaagggcc ucaucucauu uugcgaguuc     1140

gaaaguaggg agaucgaaau ugaggaaaac gguaagcgga aacgcaagaa aguggggcug     1200

aagguggccc cuaaaagcuc uccacuuuuc caggaguuua agaucuggca gguccugaau     1260

aacauugaga uaaagaauga agacgagaga agguauuugg aacccgagga aaaggagcuc     1320

cuguucaacg aguuaaauug uaagggaaac cugaccuccc agaaaugccu agaacuccug     1380

ggcuuuaagu caaaagaugc aaaacuuaau uucaaaguug uggagggcaa ccguacaaau     1440

gaaaaguugu acgacgcuuu ucugaagauc cucgagcugc aaggguauaa cguauucgaa     1500

cuucugaaaa uuaaggagga uaaagacgag gccaaguuga gcgaacucaa agcuagugcc     1560

gaugagauca agggaaugau caaggaaauu uuuaacgcaa aucugauaaa caccucuauc     1620

uuagacuuca augccgagcu ggaagguaaa gacuuugaga accaggcuuc cuaccaguuc     1680

uggcaccuaa uguauagcua cgagggcgau aauucgccuu cugggaacga aaagcucuau     1740

gagcugcuug aaaagaaguu uggauucaaa aaggagcauu ccaagauuuu gucaacgauc     1800

guguuuaguc aggaguacgg cagccugucc acuaaagcga ugcggcgaau uuacccauau     1860

aucaaggaca auaaauacuc ugaagcaugu cuccuggccg guuauaacca cagcaagcac     1920

ucauuaacaa aagaggaacu ggagaauaga aaacuuaaag aacaauugga gguccucccg     1980

aagaacaguc ugaggaaucc cgugguugag aagauacuaa accagaugau caaucuggug     2040

aacgcuacca uuuccgaaua cgggaaacuc gauggcgucc gcaucgagcu ggcccgggaa     2100

cuuaagaaaa gcgcacagga gagagaagac auggccaagu cuauuaauga ggcuacuuug     2160

cugcaucaga aauaugccac aaucuuaaag caagaguucg gagugguaaa cccuucaagg     2220

aacgauauaa uucgcuacaa gcucuauaug gaacugaccg uuaauggcua caaagaccuu     2280

uacacgaaca ccaagaucga gaaagaaaau cuguucacag auaaguauga cauugaucac     2340

aucauaccac agagucgauu uuucgacgau agcuuuucca acaaaguguu ggucccccgg     2400

cagucuaauc ucgacaaggg gaaccugacu gcauacgauu ucaugucgaa uaagggaagc     2460

gagcgugagg aacauuuucu aaacgugauc aaagagcugc ucgacaaggg uuccauuuca     2520

aaagcgaagu augaaaaacu gaagaagaaa ggccuugaga ucggggacgg auucauugaa     2580

agagauuuga gggacaccca auacaucgcu aagaaagcca aggagauacu guuugagauu     2640

acaaauagug ucgugacuac cucuggccgc aucacagaua aauuacggaa ggacugggau     2700

cucguuaaca ucaugaagga acugaaucuu gacaacuaua gaaggcuggg ucuaaccgag     2760

auugugcgaa auagcaaagg ggaagagaag gaacucaucc cugauuggac uaaacgcaac     2820

gaccaccggc accaugcaau ggaugcccug acgguagcuu ucacaaccag aaaucacauu     2880

caguacuuga acuaucugaa cgccaggaag gacgagaaac auaaggagca ccagaauaua     2940

uacgcaaucg aaaacauuau cacugagguc auugagaaaa agaauggcuc caagacccgu     3000

cgcuuuaaag cuccaaugaa cucauuccgg caugaagcca agcagcacuu aaaagaaauc     3060

cucgugagcc acaagaccaa aaauaagguu gugacaucua acauaaauaa gacuaaaaag     3120

aaaggaggca uccauaagaa aacagagcug acgcccagag ggcaacuuca caaggagacc     3180

gucuacggau ccaagaaauu ucugcagagu agggaagaga aggugagcgc gaaauucgau     3240

uaugaaacua uuuugauggu agugaacccg auccaucgaa augcccuccu gaaccggcua     3300

aaggaguacg guaaugaccc uaaaaaggcu uuuucuggca agaacgcaau uaauaaaaac     3360

cccguuuauc uggaugacuc caagacagac auccuuucag aaaaagucac ccucaguugg     3420

uacgagacag gguauacuau acgcaagagc gugaccccag auaauuucaa agacuacaag     3480

aaccuggaga aaauuacgga uuugggaauc aagaagaucc ugaaagaaag auuagaccag     3540

uucaagggca augccaaaga ggcuuuuucg aaccucgaua aggacccuau uuggcugaac     3600

gaagagaaag guauagccau caagaccgug acaauuacug gggucuccaa ugcagagucu     3660

cuucacuuca agaaagauca ccugggcaag gaaaucuugg acgagaacgg aaauaaaauu     3720

cccggcgauu uuguuagcac cgggaacaau caucaccucg cgaucuaucu ggacgaagag     3780

gaaaagcuag augacaaaau ggugacauuc uacgaggccg ugcugagggu aaaccaggga     3840

cuuccaguca uagacaagga uuacaauaag gagaaaggcu auaaguuucu caugacucug     3900

aaacaaaacg aaauguucgu guuucccaau gaggacuucg auccuaacga aaucgacuug     3960

uuagaugagc ggaaucugga acgaauuuca aagcaucucu uuagaguuca gaaaaucagu     4020

aagguggguu acgggaacuc cuucguccgc gacuuugugu ucaggcacca ucuggagacc     4080

acgguugagg aacguaagga gcuucggaau auuacauaua uccagcugaa aucuuuggaa     4140

ggacuaagaa acauaauuaa ggugaggcug aaucaccucg gcaaaaucgu acagaucggu     4200

gaguacuga                                                             4209


<210>  3299
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3299
augaccaaga acauccuggg ccucgaccuu ggggugagcu ccauuggaug ggcccugguc       60

caggaggauu cuaaaaaucc cgaaaacaau agaauaguua aguugggugu gaggguaaac      120

ccuuuaacag uggacgagca aaucaauuuc gaaaaaggca agccaauuac uacgaacgcu      180

ucacggaccc uggcacgcag ugcccgacgu aaucuacaga gauuuaaauu caggcgggcg      240

aaccucaucg auguccugaa gaagugcaau auucuuaaac agggggaccu guugacagag      300

aucggaaaga acagcacuuu ucagacccaa gaacuccgcg cuaaauccgc caaggagaaa      360

guggagcugu cugauuucgu uagagugcug cucuuaauga auaagaagag gggcuaccgg      420

ucgucacgca aagcaaagaa cgaagacgag ggucagauaa ucgaugggau gagcgucgcc      480

aaaaagcugu augaagagaa ucuuacaccg ggagaguaca guuaucaguu gcugauugaa      540

aacaaaaagc agcuacccga cuuuuaccga uccgaucucc aaucugaguu cgacagagug      600

uggaaauuuc agaagcaguu caauccugaa auccugaccg augaccuuua uaaggagcug      660

cagggcaaaa acaagaacgc uacuugggca guauugaaag aaccauuuag ccucguuggc      720

auuaagcaaa ucgggacaau acaggagaaa aaggccgaga aguacuucug gagguccgaa      780

gcugugaaac ggcagcugga uuuugaguca uuagccgucg uguuccaaga aauuaauagu      840

aaccugaaua acagcucugg auaucucggu gcgaucuccg accguucaaa ggagcuuuac      900

uuuaauaacc agaccguggg cgaauaccug uuccagcagu ugaaagagaa uagccacacg      960

aagcugaaaa accaagucuu cuaucgccag gacuaccuag augaguuuga aaagauuugg     1020

gagacucaga guaaguauua caaagaacuc accaaggagc ugaaagaaga gaucagagac     1080

aucauuauau ucuaucagag gaaacuuaaa ucucaaaagg ggcugaucuc cauuugugag     1140

uuugaaaauc gggagaucga aauaacagag aacggaaaga uuaaaaagaa aacuguuggc     1200

uugaaggugg cacccaaaag cucgccucuc uuccaggagu uuaagaucug gcagguacug     1260

aauaacuuag uguuccaguc uaucgaaucc aaagagcugu uuccacuaga ucucgacauu     1320

aaggaaucaa ucuucaauga ggucaacauu aaggguaauc ugagugccaa aaacgugcuu     1380

gauuugaugg gguacagcgg aaaggaaugg aaaaccaacu uuaagaauau agagggcaac     1440

cgaacaaaug agaaccugua uaccgcuuuc cucaaaauca uugccuccga aggcaucgag     1500

uuucccaagg aguucaagcu gucuaucgac gaugacauua aaguuagcaa gauagauauc     1560

ucaagugaga aaauuaagga auuuaucaaa gaaaauuucc uuaagcuggg gauuaacacg     1620

uccaucuugg acuucaaucc ugagcucgau ggaaaggacu uugaaaaaca agagagcuac     1680

cagcuguggc auuuacugua cucuuaugaa ggugacgauu cagcaagugg caacgugaag     1740

cuauacgagc uccuggagaa aaaguucggg uuuaaaaagg aacacagcaa gauacuugcu     1800

gacgucggau ucuccccaga uuauggcucu uugucgagca aagccaugcg caagaucuac     1860

ccguauauua aagagaauaa guacuccgac gcgugccauc uggccgguua uaaccacagu     1920

aaaaauucau uuacuaagga ucagcucgca aacagaaucc ugaaagaaca guuagagauu     1980

cugcccaaga augcucuuag gaacccugug guagaaaaga ucuugaauca aaugauaaac     2040

cucguuaacg aggugauuaa agccuacggg aagccagacg agauccggau ugaacuggcu     2100

cgcgagcuga aaaagaaugc cgaagagaga gcaaacauga caucugauau cagcaaagcc     2160

acccuacucc aucagaagua cgcugaaguc cugcagaagg aguucggcau aaaagcgccc     2220

uccaggaaug acaucauucg uuauaaacuu uacuuggagc uggagaagaa cuuauuuaag     2280

gaucucuaua cugacgugca aaucaaacgg gaagaacugu ucacagauaa guacgacauu     2340

gaucacauca uaccucaguc acgauuuuuc gacgacagcu uuucuaauaa gguccuugug     2400

ccaagaggag caaaccugaa aaagggcaau uucaccgccu uggauuauau ggagauugaa     2460

gggaaagaga agcucgagaa auuucugaac aucaucaagg aacuauucga caagggagcu     2520

auuuccaaag ccaaguucga gaaacugcag aagaaaggua gugaaaucgg cgagggguuu     2580

auugaaaggg aucuccgcga cacgcaguac auagcaaaga aggccaaaga gauccuguuc     2640

ggaauuacca auagcguuau cucuacaucc ggccggauua cugauaaacu uagagaggac     2700

uggaacuugg ugaauaccau gaaggaacug aacuuagaga aguauaggaa acucggucug     2760

acagaacuua ucguaaauuc aaagggggag gagaaagaac gcauaaucga uuggacuaag     2820

cgaaacgacc accggcauca cgcuauggau gcccugaccg ucgcauuuac aacccauaau     2880

cacauucaau accuaaacua ccucaacgcg cguaaggacg agaaacacaa ggaacaucag     2940

aauaucuucg cuauugagaa cacuaucacg gaaauagugg agaaaaagaa uggcaguaaa     3000

gagagaaggu uuaagcugcc cauuaagaac uuccgcacag aagccaaaaa gcaguuggag     3060

gaaguucuga ucagccacaa agcaaagaau aaagugguca ccaagaacau caauaagauu     3120

aaaaagaaag gaucgauaau cgccaagacu gaguuaaccc cgcggggcca gcuccauaaa     3180

gaaacaauuu auggguccuc uaaguuucug aagaccaaag aggagaagau cagcggaaaa     3240

uucgaucuug aaaugauuca aaaggugcag aacgagaaau acagaaaugc ucuguugaag     3300

aggcuccagg aauuuggugg cgacucaaag aaggccuuca cugggaagaa cguaaucagu     3360

aaaaauccua uauuucugaa cgcagagaag acagaacagc uacccgagac ggugacccug     3420

gcuugguaug agacuggaua cacaauccga aaaguuguca auccagauaa cuucaaggac     3480

uucaaaaaua uugaaaaggu gaucgacaag ggcgugcgcg auauucuuac cgagcggcuc     3540

aaagaauuua acgguaauuc caaggaggcc uucucuaacc uggagaaaaa cccuaucugg     3600

uugaauaaga gcaaagggau auccauuaag acagucacua ucaccggcau caacaaugcg     3660

gaagcccugc acuauaagaa agaccauuua ggaaaggaga uucucgauga aaacggccaa     3720

agaaucgcug uugacuuugu gucaacgggg aauaaccacc acguggcaau auacgaggau     3780

gacaauggaa accugcagga aaaaguaguc agcuucuaug aggccgugga gaggguuaau     3840

cagaaccuuc ccauuaucaa uaaggaauac aacuuugagc ugggcuggaa auucuuguuu     3900

accaugaagc aaaaugaaau guuccucuuu ccaagugaug acuucgaucc caaggaggug     3960

gaccuguuug augacaaaua ccuaucucug auuuccaaga accuuuuccg uguccagagc     4020

aucucgauag ugcaguaugg uaauucaguu auucgcgacu uuaaauuccg gcaucaccuc     4080

gaaacaucug ugagugauga gaagaaacug caggggguaa cuuaccaaca gaucaagucc     4140

uugcagccuu uaaaagacau cauuaagauc agacugaaca accucggaaa gauuguccag     4200

auaggcgagu auuga                                                      4215


<210>  3300
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3300
augaagggca acaucgagaa aaagaaacag gaguucaaug acuacuaucu gggguuugau       60

auuggaacca acagcguggg uugggccguc uccgaccucu cuuacaaucu ucugagauuc      120

aacaagaaag auaugugggg cgcuagguug uuugacgaag caaagacagc caaggaucgg      180

cgcguuaaac gaaauucacg uagaagguua gaacggcgca guaagagacu gaaauaucua      240

gaggaaauau ucgagacuga gaucugcaag auugacaaaa acuuuuucag gcggcucgaa      300

gagagcacgc ugcacaauga agauaagacc uuugacuucc gcuuucccau cuucaacgau      360

gagaauuaca cagacaagga guauuacaaa gauuuuccua cuguguauca ucuucgaaac      420

gaacugauua agaaugagaa caaaaaguuc gacaucagau ugauauaccu cgcgauccac      480

aauauucuga aauacagggg gcauuuuauc uucgaaggac aagauuucga cuccguaacc      540

gacuuuaagg agguguucuc ugaacugucg gcuuaucucu cagagaacuu agaggaaaau      600

cuggauauua accuuuguga caacaucaaa gauauaauuu gcagcaagga gaauaagaca      660

aguaaaggca aguccuuugu cucuuugauc gacagcaaag ugauucaguc cguucugcgg      720

uguugcaucg guuaccgugu gaaguucaaa gauaucuuua uuaacuauga agagaaggaa      780

aagaaugagg ggauaaaccu aucagagauc gaauacgacg agaaacgcga ugaaauugcc      840

cucagucugg gagagaauau ccuucugaua gacauuugua agaaagucua ugauuucaug      900

aucuugaaca auauccucaa gggcaacaaa uacauuagcg aaucuaaggu gaaucuguau      960

gacgagcaca agugcgauuu auguaaccug aaauacaucu uuaagaaaua ccucccaaag     1020

aaagacuauu ucgaguuuuu cagaaaugaa aacaaggaga agaauuacgu auccuauauu     1080

ucaagcaguc uuaacaaugg caaaaagaaa aauaccugca cucaggaaga ccugaacaag     1140

gagauaaaag aguacaucgc aaagauuaaa gaauaugagg aaaacgaugc cguuuuggag     1200

aagaucaacc uaaagcugaa ugaaaacaaa cuccugccga aacuuaaaac aaaugacaac     1260

ggggugaucc ccuaccagau ucauaagaua gagcuggaga aaaucuugca aaaucagucu     1320

aaguauuacg auuuucuccu ggaaaagauu gacggaauca ccaacauuga gaggaucgaa     1380

aaauuauuca cguuucggau accuuacuau aucgguacuc ugaagggcaa auucuccugg     1440

gcuaagcuaa aagagaauaa gaagauuacc ccauggaacu ucgaaaagau cgucgauaug     1500

gaggagagcg caaaggccuu uauugaacga augucgaauc gcuguucuua ccucaaaaca     1560

gagaacgugc ugcccaaaaa uucccuuuug uauucagaau acgagcugcu caacgaacug     1620

aauaaaguga agaucaagaa cgaguucaua aacgucguuc uuaaaaauga gauuaucgac     1680

gaacuguuua agacuaccag uaaagugaca aagaaaaagu ucguagauua uuugaaaacc     1740

aagcagauuu uuguggagaa ggaagacauc agcgggcucg auaacgaguu caaauccaag     1800

cuggacucuu acauaaaauu uaagaaaguc aucggagaua agauugacac guuaagaggc     1860

uauaauuucg uggagaagug caucgaauuu aaauguuucu acggugauga caagaaacug     1920

uuuaagaacg cuauuaauaa cacugaguuc aaagacuacg uuagcgauga cgaacuaaag     1980

aagaucaaua aaaugaaguu uaacaaaugg gggaggcucu caaagcggcu gcuuacagag     2040

auaugcuucg aaaaugugau ugagagugag uccuucgaaa acaucaucgu cgccuugcgc     2100

agcaccaauu auaacuuuau ggaguucacu ucuaaaaagu uuaaucuguc agaaaaggug     2160

gcggaggaaa accaggagaa uacacucgag aaauucaaca uuaacgaaua cguagaugag     2220

cuguauguua guccuagcgu gaagagaucc aucauucaaa ccuacaaaau agcaauggaa     2280

aucaagaaaa uuacgggaaa gaauccaaag aaaaucuuua uugagauggc caggggcggc     2340

ggggaaaagg ggaaaaagac cgagaaacgu aaggagcgga ucaaaacauu auaugcuaac     2400

augaaggacu cuaaugaaga guuccugaac aaucuugaua acuugaagcu cucggucgac     2460

aauuacgaug acaaccgacu gagagaaaag aagcuauuuc uguauuucau gcagcucggu     2520

aaaagcaugu acacuggcaa gaauauagau cugaacgagc uuuuguuugc caauaccuac     2580

gacaucgauc acauuauccc ccaguccaaa cugaaggacg auucauucga caacauugug     2640

uuagucgaga agacaaucaa uaaagacaag gauaacaggg acuaucucga ugaaaguaua     2700

cgccugcgga ugaauaaauu uuggaacuuc cuuagagaga agggguucau uacuaucgaa     2760

aaauacaaua ggcugcgccg aaacaccuau uuuucugacg augaguugag cggauucauc     2820

ucccggcagc ucgugacaac ccgucaauca acucuggaag uuaagaagcu auuugaguuc     2880

auuuuucaga acacggacgu gguaugcagc aaagcaaauc uggucucuga uuucagagag     2940

aaguacggca uaaucaaagu gagggaccuc aacgaugcuc accaugccca ggacgcguau     3000

cugaauauug uuguggguaa cgucuuugau acaaaguuca ccaaaaauua cuggaaguuu     3060

aucgaagacc gccggaaggu ggacaacauu agagagacuu auaauuucaa aaagaucuuu     3120

gauuacgacg uauccagggg agaaaacgug gccuggcuua aagauuacag uauaaauacc     3180

aucagcgaga ugauuaacaa gaaaacagca aauaucaccc aaaugauuac ugaaggcaag     3240

ggacaguugu ucgaccugac aauccucaaa aagucuaaga uagauaaaaa guuaacgguu     3300

auuccuaaca ccccaaaaau ccugaagucc ucacuuaaag acaguuguaa gaucgcugag     3360

aaguauggcu acuuuagcac ucugaugccg gccuauuucg ucguguucaa auacauugau     3420

aagaauaaag agguguuugg guucgaccga aucuuuauau ucgaucgcga aaagauuaac     3480

ucgaaugagg acaucgaaaa auaucuagag gauaagggau acaacucccc caaguuuauu     3540

cggaaaguca accucaagca gaaaauccug uugaaugguu auccuuacag aauaguuggc     3600

uucacagaca acaagcugga aauuaaaaau ucuaagcagu uuuacagcga uggggagcac     3660

uuaaaguaua ucaaacaucu cucagcuuuc uacaacaaua acaauaacgu gaagaaugga     3720

gaguauagga ucaacuuucu gaaaaagacc agugaaaaug accguaacga gacaacugac     3780

gaagccaaag agcgccuuaa uauugaguuc aucaagcugu acgaauauuu uugcgagcgg     3840

uugaacucca aaauuuacgc aaauuauuac aacucuaaga aguacgauga acucauagag     3900

guguucgacu guuuuaaaaa ccugaaucua cuggauaagg ccaaacuucu ccuggaguuc     3960

uugaagaucu ucaaaaagga caccuauggc aacuuuaagc uguuagaaaa accagagcuc     4020

gauaagaaug guaaacaaaa gcuggacaaa aacgggaauc uuagcuucga aggccugucc     4080

auggcuuuug gaaaguugaa cggcaagaaa uucaagcagc ucguagagaa agauauucag     4140

uuugucgacg aaucagugac ggggcuguuc gagaagaaag aaggacuaga gaaguggcag     4200

gauggugagc aauga                                                      4215


<210>  3301
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3301
auggccaaga aaauccuggg ccucgaccuu ggggugagcu ccauuggaug ggcuauaguc       60

gaggaagaug cagagaacgu uaugaauaac aagaucguga aacugggugu aagagugaau      120

cccuugaccg ucgacgaaca gauuaacuuc gagaagggca aaccuaucac aacuaaugcc      180

gggaggacgu uagugcgggg agcgcgccga aaccugcaac guuuuaagcu aagaaggcuc      240

caccugauug auauccuuca gaagcggaau auacugaaac aggcugacuu gcucgccgaa      300

ucuggccgca acucaaccuu ccagacacaa gagaucagag caaagagugc caaacagaag      360

guugagcuga gcgaacuggc uagggugcuc uuacugauua auaaaaagcg ggguuacaag      420

uccucucgca aagcaaagaa cgaggaugaa gggcagaucg ucgacggaau gucgguggcc      480

aaaaaguugu augaggagaa uacuacccca ggcgaauacu cauaugaguu gcugaaaagc      540

ggcaagaaac agcuaaguga uuuuuaccga uccgaccucc aagcugaauu ugauagagua      600

uggaacuuuc agaagcaguu cuauccggag auuuucucug acaaucugua caaggaacuu      660

caggggaaaa acaagaaugc cacauggaaa auccuggaga uacccuucag cuugguugga      720

auuaagcaag uggguaccac ucaggagaaa aagaucgaaa aguauuuuug gagguccgag      780

gcggucaaaa agcagcucga uuucgaauca cuggcagugg uguuucaaga gauuaacagu      840

aacuuaaaua acagcucugg cuaccugggg gccaucuccg accggucaaa agaacucuac      900

uucaauaacc agacagucgg agaguaucuu cauugccagc ugaaggagaa uccucacacc      960

aaauugaaga accagguguu uuaccgucaa gauuaucuag acgaguucga aaagaucugg     1020

gaaauucaga gcaaauacca uacggugcug acuaaggagc ucaaagaaga gauacgcgac     1080

guaaucauuu ucuaucagag aaagcugaaa agucagaagg gccuuaucuc uauaugugag     1140

uuugaaaaua gggagauuga aaucaccgag aacgguaaga ucaaaaagaa agaggugggg     1200

cugaaggucg cuccaaaauc cagccuucuc uuccaagaau uuaagauuug gcaagugcug     1260

aauaacuuac aguuccagaa uaucgagucg aaagaaauuu uuccccugga ucuagacugc     1320

aaggagucua uauucaacga aguuaauauc aagggacgga uuuccgccaa agcagugcuc     1380

gauguccugg gcuacucagg caaggagugg aaaacaaacu uuaaggacau cgaggggaau     1440

aacaguaacg aaaaucuuua uaacgcuuac uugaaaauca uugccaauga gggaauagag     1500

uucccuaagg aauuuaagug uaaccuggau gacgauauca augugagcaa aauuaucugc     1560

uccgcgggua agauuaucga guucguacga gaaaaauuuu cugagcucgg cauaaacacu     1620

agcauccugg acuucaauuc agaacuugag gggaccaacu uugaaaagca gaaaaguuac     1680

cagcuguggc acuugcucua uuccuacgau ggagacgaua gcccaucugg caaucagaag     1740

cuguaugagu uacuggaaaa gaaauucggu uucaagaaag agcauucaau uauccuagcc     1800

gacauugggu uuccccagga guacggcagu cucagcacaa aggcaaugcg caaaaucaua     1860

ccucaccuga agaccggacu uccauucgcu aagaaauccg acaacgauac gauuuugucu     1920

gccacugcua uggccggcua uaaucacagc aagucgcuga caaccgagga acucacuaaa     1980

agaguucuga aggaacauuu agagauccug ccgaaaaacu cccuuaggaa ucccgugguc     2040

gagaagauuu ugaaccaaau gaucaaugug gucaacgaag ugauaaaaga guacgggaag     2100

ccugacgaaa uccggauuga gcucgcacgc gaacugaaga aaaacgccga ggagagagcu     2160

aauaugacau cagaaaucgg aaaggcgacc auucagcacc agaaauauag ugagauccua     2220

caaaaggaau uugguauaaa aucuccaagc aggaacgaua uuauccguua caagcuguac     2280

cucgagcugu ccaauaacgg cuucaaggac cuuuauacgg auauccagau uaaacgggaa     2340

gaguuguuua ccgacaagua cgauaucgac cauauuauac cccagucacg auucuuugau     2400

gacagcuucu cuaauaaagu ucuggugccu agaggggcaa acuuaaagaa aggaaaucuc     2460

acagccuuug auuaucugga guccgaaggc aaggacacuc uugagaaguu caucaacacc     2520

auuacagaau uuuacgauaa agguaucauu acuaaggcua aauaugagaa gcugcagaaa     2580

aaggggaccg aaaucggcga cggauucaua gagagggacu ugcgcgauac acaauacauc     2640

gccaagaaag caaaggagau ucucuuuggc aucaccaaua guguagucag cacuucuggg     2700

cggauuacgg acaaacugag agaagauugg aaccuaguga auacaaugaa ggagaugaac     2760

cuggaaaaau auaggaagcg cggacucacc gagacuguua ucaauuccaa gggugaggaa     2820

aaagagaaga uaauugacug gucaaaacga aacgaucacc ggcaccaugc cauggacgcu     2880

cugaccgugg ccuucacaac ccacaaucau auccaguacc uuaacuacuu gaacgcacgu     2940

aagaaugaag aucacaaaca gcauaagguc aucaguaaca uugagaaucu gauaacuaaa     3000

guguucgaaa agaagaacgg cagcaaacag agaagguuuu uagagccaau ccacaauuuc     3060

cgcauugacg cgaagaaaca ccucgaggaa auccugauuu cgcauaaggc uaaaaacaag     3120

guagugacaa agaauaucaa caaaauaaag aaaaaggggu ccaucauugc caaaaucgag     3180

cuuacgcccc ggggacaacu gcacaaggaa accauuuaug gcucuagcaa guaccuaaaa     3240

acuaaggaug agaaacucuc agguaaguuu gaccuggaaa caaucaauaa aguucagaac     3300

gagagauaua agaaugcauu gcuggagagg uuaaaggucu ucggguacga uaguaaaaag     3360

gccuuuaccg gcaaaaacau acucgcuaag aauccgauuu uccugaacau cgagaaaaca     3420

gaacagcuuc cugaagugcu gacuuuggcc ugguaugaga ccaucuacac gauucgaaag     3480

uccaucaauu cugacaacuu uaaagauuau aagaauauag acaaggugac cgacaaaaag     3540

guccauaaca uucucacaga ucgccugcgg gaguucaacg gaaauagcaa agaagcauuu     3600

uccgaccuag agaagaaccc caucuggcug aauaaaccaa agggcauugc uaucaagacu     3660

guuaccauaa caggggugaa caauguggaa gcccuucacu acaaaaagga ucacuucgga     3720

aaagaguuuc ucgacgaaaa gggccauaaa auuccuguag auuucgucuc aacugguaac     3780

aaucaccaug uggcgaucua cgaggacacc gccgggaacc ugcaggagag aguuuugagc     3840

uucuaugaag cuguggagag ggucaaucaa ggacugagug ugaucgauaa ggaauacaac     3900

ucuuccuuag gcuggaaguu ucucuucacg augaaacaga augagauguu ucuguuccac     3960

agcgaagacu uuaaccccca ggagauugau cuuuaugacg agaagaaucu gcguuugauc     4020

ucgaaaaacc ucuuccgcgu ucaaaaguuu acagugaaag auuacuucuu ucggcaucac     4080

cuggaaacca auguagagga caacucaaag cuaaagggug acacuuggaa aagagaaggc     4140

cugaacggga uuaggggaau acuuaagguc cgacucaauc accugggcaa aaucguucag     4200

gugggggagu auuga                                                      4215


<210>  3302
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3302
augagcaaga aaauccuggg ccucgaccuu gggaccacau ccauuggaug ggccuucgug       60

agagaggcug aagauuuuca ggaggaaucu ucaauagaga ggaucggugu ccggguuaac      120

ccccugacua cggacgaaca aauuaauuuc gagaagggca aaccuaucag ugugaacgca      180

gaucgcaccu ugaagcgagg ggcccguaga aauuuacaga gguacaaaca gcggcgcaca      240

aaccugauuc agcuacucaa gcaauauaag aucauaaaug agaacacuau ccuggcggaa      300

gacgauaaaa auaccacaca cagcaccuac gcucuuagag ccaaggcagu auccgagaaa      360

auuucuaagg aagccuuugc uagggugcug uugaugauca acaaaaagcg gggauauaag      420

ucgucacgca aagcaaagaa ugaggacgag ggccaggcca uugaugguau gagcgucgcu      480

aaacgacucu acgaagagga caucacucca gggcagcugu gccugcagcu cuuaaaggaa      540

ggcaaaaaga aacugccgga uuucuaucuu agugacuugc aagccgagcu ggaaaagaua      600

uggaacuuuc agaagaaauu cuaccccgag auucuaacag augaguuuaa gaaagaaauc      660

gagggaaagg gccagagagc gaccuccgca ucuuucuggg ccaaauauaa guuuaauacg      720

gcugaaaaca aggggagcag ggacgagaaa aagaugcagg ccugggaaug gcgguccaaa      780

gcaauuaaua cucaacucac ccgugaggag guggcuuacg uuaucacaga aaucaacaau      840

aacauuaaca auucaagugg auaccugggu gccauaagcg aucgcucuaa ggagcuuuau      900

uucaacaaag aaacuguggg cgaguaccug uuuagacagu ugagguauuc cccucauacc      960

cgggucaaga aucagguguu cuaccgacaa gacuaucucg acgaauuuga gacaaucugg     1020

gagaaccagu cacgcuacca caaugaacug accaacgagu uaaagagcga aauuagagau     1080

guaguuaucu ucuaucagag gaaacugaag agucagaaag ggcucauauc uuucugugag     1140

uuugagucca agcaaauuga aauccagaaa gacggaaaga ucgugaagaa aauuaucggc     1200

cuuaagguca gcccaaaauc gucuccccug uuccaggagu uuaagauuug gcagauauug     1260

caaaaucuag aguucacgaa caaagaaacu aaggagaaaa aggugccuga uuuugaagcg     1320

aagaaucuac ucuucgagga acugaacauc aaagguaauc uuaaggggga gaaagccauu     1380

gaaauccugg gauacaagcc aaaagagugg uuggugaacu acaaggaacu cgacggcaau     1440

cggacaaacc agguccugua ugagggcuac uuaaagaucc uggagguuga aggguaugau     1500

auuaaagacc uacucaagau aaaauccaau aaggaugagg ugaaccuguc agaacuuagu     1560

aaacccguaa gcgagaucaa ggacuugauu aaguccaucu uugaaucucu gaacauuaau     1620

accgcaaucc ucgauuucaa cccugagcug gagggaaaag acuuugaacg ccaggcuagc     1680

uaccagcuuu ggcaucuguu guauucauac gagggugaua auaguaacac uggcaaugac     1740

gaacucuaua gacuguuaua ccaaaaguuc ggguuuggaa aagagcacgc ccagauacug     1800

gcuaaccuau ccuucagcca ggacuacggc ucucucucag ccaaggcaau gaggaaaauc     1860

uauccauuua uuaaggagaa uaguuacagc gaagccugcu cccuggcugg uuauaaccau     1920

ucuucgagca ucacaaagga ggaaaaugau aaacgugagc uuaaggaaaa auuggagcug     1980

cucccgaaga acucccugcg gaaucccgug gucgagaaaa uuuuaaacca gauggugaau     2040

guuaucaacg cgauaauugc agacccuuca auggggcgac cagaugaaau cagaauugag     2100

cuggccaggg aacuuaagaa aaaugcugcc gagcgcgcag aaaugaccau ccacauaaac     2160

aaggccaaga gugagcacga caacuaccgg aaaaucuuga cgaccgaguu cggcauuccc     2220

aauccuacaa gaaacgauau cauuagguau aagcucuacg aggaacugaa agugaaugga     2280

uauaagacuc uauacucuaa caccuacauc ccacgcgaaa aacuguucag caaggaguuu     2340

gacgucgaac auauaauucc caaggcucuc cuguucgaug acuccuuuuc aaauaaaaca     2400

aucugugccc gacaagagaa ccuugauaag ggcaauaaaa cugcauucga cuuuauccag     2460

agcuucuaug ggaaggagaa auuugaagag uacuugcagc ggauugaauc ucuggcgaag     2520

aucaaggagg gagauaccuu cgugauuucc aaagcuaagu auaaaaaguu acuccugccg     2580

gagauagaaa ucggugaggg cuuuauugaa cgugaccuua gaaacaguca auacaucgcc     2640

aaaaaggcaa agagcaugcu guuggauauu ugcaaaacag uaguuccuac cacugggucu     2700

aucacggaca ggcuccgcga ggacugggga cugauaaaua ucaugcagga acuaaacuuc     2760

gagaaguaua aaaagcuggg ccucauugag augguggaga aaaaggaugg uuccuuuaag     2820

gaacggauca cagacuggac caaaagaaau gaucacaggc aucacgccau ggacgcucug     2880

acugucgccu ucaccaagca caaccauauu caguaccuua auuauuugaa cgcacgcaaa     2940

aaugaaaacc acaagaugca ugggaacgug aucgcuauag agcagaaaga aacagagcug     3000

uuagucgauu cagugggcaa gcgaaaacgg aaguuuaagc uccccauuag uaauuucaga     3060

gagguugcca aaaugcaccu ggaaaacauc cuugugagcu acaaggcgaa aaauaaggua     3120

gccaccaaaa acaagaauag guucaagacu aaaaacggag agaaggucaa aaucgaacug     3180

acaccacgug gccaacuaca uaaggagacg guguacggga aaaugaagaa guaugaaacc     3240

aaagaggaga uuauaggauc gaaguuugac gaagcuacua uccucaaagu ugcaaaucug     3300

gagcagcgca acgccuugcu gcagcgguua aaggaaaaua acaaugaucc uaaaaaggcu     3360

uucuccggua agaacucucu caaaagcaau ccccuguacu auggcgugga cucaaagcca     3420

auucccgaga gaaucaaaau ugucuggcuu gaggaugacu acacaaucag gaaggaaaua     3480

accccugauc ugaaaaucga caaggugauu gauaucggga uucgacggau cuuggagaac     3540

cgccucgccg aauuuaaugg aaacgcaaaa gaggcguuca guaaucugga aaagaaccca     3600

auauggcuaa acgagggcaa gagaauugcc aucaaaaggg uaacaaucuc cggugugaag     3660

aaugcugaga cucugcacua uaaaaaggac caccuugggc augaaaugcu cgacaaacag     3720

ggcaagcgga uuccgguuga uuuugucucu accggaaaca aucaccaugu ggccaucuac     3780

aaggacgaua aaagcgaccu gcaagagaag guggucuccu ucuaugaagc aguugagcga     3840

auaaaccagg aauugcccau uaucgauaaa ucauuuaauc acgaccuggg cuggcaguuc     3900

uuauuuacga ugaagcagaa cgaguacuuc guguuuccua gagauggguu cgacccauac     3960

gagauugauc uccugaaucc cgacaacuau aaaaucauaa gcccuaaucu uuuccgcgug     4020

caaaagauug cuaccaagaa cuacguauuu aaucaccauc uggaaacaaa agccgucgau     4080

ggagaguugc ucaagacuaa aaaggaccug agugacauca ccuauagguu cauccagaca     4140

ccagugaacc ugaaaaauau uaucaagguu cguauuaacc acauaggcca gaucgugaag     4200

auuggugaau acuga                                                      4215


<210>  3303
<211>  4218
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3303
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc ccuggugaaa       60

gaggcugaaa auuccaacga gaagucugaa auaaucaaau ugggugucag aguuaauccc      120

uuaacugugg augagaagac gaacuucgaa gcaggcaggc cucugucaac caaugccgac      180

cggacagcga aacgcagugc ucgacguaac cuacagagau acaagcucag gcggaagaau      240

cugauugauc uucugaucaa aaaccaauug auugacaagg auacuccacu caccgagauc      300

gggaaaaaua caacccacca gacucuggag cugcgcgcca aggcagccag agaaaggaua      360

gagcucgaag acuuagcucg gguauuucug gcaaucaaca aaaagcgcgg auaucgaagc      420

uccagaaagg ugaauaacga ggaggaaggc caggccgucg augguauggc uguggccaaa      480

aaguuguacg acgagaauuu gacaccgggg caguaugcgu acgaacugcu aucuaaagga      540

aagaaauaug uucccgauuu cuacaggucg gaccuccaag cagaguuuaa cgccauuugg      600

gaauaucaga agcaguucua cgcugauauc cuggacgaug agcuuuacaa ugcccugaaa      660

ggccagggcc aacagaacuc acggaagcgu uuucuugcaa uuaagggggu guauaccgcu      720

gagaacaaag gaaagcgcga cgacgucaaa cucagacauu acaaguggag gagcgaagcc      780

aucaguaaaa agauaaauau ugaggaagug gcguauguac ugguugagau caacaaugau      840

uuaaacaagu ccucugguua ccugggcgcc auuagcgacc gguccaaaga acucuauuuc      900

aauaaggaga cgguggggga gaaccuuugg aaacagaucc agaagaaucc ucacacuuca      960

cugaaaaacc aagucuuuua ccgacaggau uauuuggacg aguucgagca gaucugggaa     1020

acccaagcac aguuucaucc acagcuaaca cuggcucuca aggagcagau ucgcgaugug     1080

auaaucuucu accaaagaaa gcugaaaagu cagaagggac uucugagcuu uugccaguuc     1140

gaaucuuggg agauugagag gaaagacgaa aauggcaacg ugaucuugaa uaagacuacc     1200

cagcucccca aacggcaaac agucggucgc agaguugccc cuaaguccuc accacuguuu     1260

caggaguuca agauauggca gaacauuaau aacuuagaag ugaccaaaau cagcgagggg     1320

gcuaguaaga auaaaaagga gacggaaacu cugucugaug acgagaggaa acuacucuuc     1380

gaagaacuga accuucgugg aaacuugucc cagaaggaag uacugcaaau ccucggccug     1440

aaagauaagg aguacaagac aaauuuuagc gagggccuug aagggaaccg gaccaaugcc     1500

gcacuguuca acauuuauca gcagaucgcc gagaaugaag gauacgguuu ugacuggucg     1560

aaaaagucug cucaggagau uaaagaagag uugaaggcgg uguuccccca aauaggcauc     1620

caggcagaua uucucgacuu uaacgccaau cuggacggga aagaguucga aaaccaggcu     1680

uccuaucaau uauggcaucu gcuauacuca gccgaggaag augacaagau caaugaggaa     1740

gaucagauca uuuauggaaa cagugcaguc agccucaaga aaaagcugug ugagaaauuu     1800

ggcuucacuc cugaguacgc caaguggaua gcuaaugugu cccuucagga cgauuauggu     1860

aacuugucua caaaagccau gcgaaagauc auuccauacc ugaucgacgg gaaugauuac     1920

agcgaagcau gcgcgcucgc uggcuauaac cacucaaaua gucugaccaa ggaggaaaac     1980

gacaacagag aguuacugga uaaacuugag uugcucccga aaaauucccu gaggaacccc     2040

guuguggaaa agauucuaaa ucagaugguc aacgugguaa aucaaguuau cgagacguac     2100

ggaaagccug acgaagugcg cauagagcug gcccgggaac ucaaaaagag cgcagagaag     2160

agagccgaga ugaccaaauc uaucaacgaa gcuacaaggc gcaaugagga uauuaagaaa     2220

cugaucacua aggacuuugg cauuccaaac cccaccaaau cagaugucgu gcgauaucgg     2280

cuuugggaag aguuggcccc ucuggcauac aaggacgucu ucacagggaa acagaucaag     2340

aaggaagacu uauuuaguag caaaauagau auugagcaca ucauuccaaa ggcucuccug     2400

uucgacgauu ccuuuucuaa uaaaacucuu guguuccgug agaccaaccu gaagaaagcc     2460

gacagaacag cguaugauuu caucgaaucg gacuacaaug ccaccuugga ugacuauaua     2520

cagaggguug agacucucua caacaauacg aagggaacaa ucagcaaggg uaaacgcaac     2580

aagcugcuaa uggcucagaa aaaucugccc gauggcuuua uugaacggga ccucagaaac     2640

ucccaauaca ucgcaaagaa agccaaggcu augcuguauc agguguucaa ggauguaaac     2700

gucaccucag ggaguauuac ugacaggcuu cgagaggauu gggacuugau caaugugaug     2760

aaagaacuga acuuuccgaa guacaaagcc uuaggacuca ccgagauaga ggaacgcuau     2820

gacauuggcc aggagaagac aaaacggguu gaagugauca ccgauuggac uaagagaaau     2880

gaccacaggc aucacgcaau gaacgcgcug acagucgccu ucacgaccuc uagccauauc     2940

caguaccuua auuaucugaa cgcucguaag aaugaguccc acaaagagca caagaacauu     3000

aaagguaucg aaaauaagau uacucauaaa caaaaggaua agcgcaaauu uauaccuccc     3060

augccaaacu uccgggagga agccaagaaa cacaucgaga caauucuagu gucaaucaag     3120

aauaaaaaca agguauacac caaaaauauu aacaagacaa agaaaaagag cgggcauaau     3180

acuaaagaac agcucacccc uagaggccag cugcacaagg agacggugua uggaaaaucu     3240

aagaggccca uguccaagcc aaccaaaauc aacaagaauu uuaguuugga gcaagcacag     3300

cugauaauca acguucagga aaaagaguua guccuccgac aucuggcuca guucgacaac     3360

aaucccgccg uggcauuuga uacaaagacu cuuaaaaaga ugccucuguu gaagaacggc     3420

gaaccacuca aagaggugcu guguuucgaa gagauuuuua ccauccggaa ggacauuaca     3480

ccggaucuaa aaaucgagaa ggucguugac gagaaaauaa aggccauucu ggaacaacgc     3540

aagaaagauu ucgggggaaa ugcuaaggaa gcguuuagcg accuugauaa aaaccccauc     3600

uggcucaaua agaaaaaggg uaucugcauu aaaagaguga cuauaaccgg cgugucuaac     3660

gccgaggcac ugcaccacca gaaggaccau uuggggaggg aaauccugga cgauaaugga     3720

aacccuauuc cagcugacuu cguauccacg ggcaauaacc accauuuagc cgucuaccuc     3780

gauucagagg guaagcugca ggacaauauc gugaguuucu acgaggccgu ugugcggaca     3840

agccaggggc uucccgucgu ggauaaaggc cugaacaagg acuugggaug gaaauuucuc     3900

uucucgcuga agcaaaauga aauguuuguu uucccuaacg augacuuuga uauuaaugac     3960

uucgaucuac uggacgauaa aaaggcugag cuuaucucca agcaccucua ucgagugcag     4020

aaaauaucua agguaagcgc aggcaacuca uuugucagag acaucguguu ccgccaccau     4080

cuggaaacca cugugaaaga ggucaaggag uugaaagaca uuaguuacau ccaguuaaag     4140

ucccuggaag ggcucaggaa cauuaagaaa guucguauca aucacauagg aaagauugug     4200

cagguaggcg aguauuga                                                   4218


<210>  3304
<211>  4221
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3304
auggagaaga aaaucauugg cauagaccug ggggugacca gcaucggaug ggccuacguc       60

auugaagaug agaacgguca cuccagaauc cucaggauug gcacacgggu uaauccccuu      120

acuacggagg aacugaagaa cuucgaacag gggaaaccua ucaccgagac agcugagcgc      180

acuuugaagc gaucuaugcg uagaaauuua caaagguaua aacagcggcg caccaaccug      240

auaaaugugc uacucaagaa caagcugauc acagaaacca cugcacuugc cgaggacgga      300

aaaggcacaa cccaucugac gcacgcguug agagcuaagg ccgcauacgc ccagauucca      360

cucgaagauu uugcuagggu acugcugaug cucaauaaaa agcgggguua ucgcucaagu      420

cgaaaaguga agacugaggc agacgggcag gccaucgaug gaauggcugu cgccaagaaa      480

uuauacgagg aaaacauuac cccgggccaa cugugcuaua cacuuuugcu gcagggcaag      540

aaaacucuac ccgacuucua cagaagcgau cuccaggugg agauggaaaa gguuuggacc      600

uuucagaaac aauuccauca ggcgauccug acagacgagu uuaggaagua uauagcaggg      660

aaaggacagc gggccaccgc ugccuccuuc ugggguaagu acggcuuuaa uacggcagag      720

uucaaggggu cucgugaaga gaaaaagcua aaaucguacg aauggcgcuc acaggcuauu      780

acuacaccuc ugagccaaga ggaaguggcc gcggucauca ccgauauuaa caguaauauc      840

gccaacuccu cuggauauuu gggcgcaauc agcgacagau ccaaggagcu cuuuuucaau      900

aaacagacug ugggucagua ccaauauaac cuguuacagc agaauccaca cacaaaccug      960

ucagggcagg uauuuuacag gcaagauuau cucgacgagu ucgaaaccau uugggagacg     1020

caggcuaccu uucauacaau gcuuacuccc agucugaagg aagagauacg ggauaucguu     1080

auuuucuacc agcgaaaguu gaaaagccag aagaaacuug ugucuaucug ugaguucgaa     1140

aagcgcauac uggaaccucu ccaaggagac aaagucgugg gcuauaaggu ggccccaaag     1200

uccucacccc uguuucagga guucaaaauu uggcaggcuc uuggcagaau ggagauccag     1260

cacaagguca ccaaagaaag gcacuuucug gacuuggaug ccaagcaacu ccuguucgac     1320

gaguuaaacg uuaaagggga ucugacaaag gaaaaugcac uaacuauccu cggauuuaag     1380

ccuaaagagu gggagugcaa ccugcuuaag uuggaaggua aucggaccaa ccaggcccug     1440

uacgaggcuu accucaaaau ucuggaacuu gagggcuaug aagugaagga ccuguugggg     1500

cucaaacugg auaaggacga uaucaaauua agcgagcugc caguagacgc ggcagauauu     1560

aagaagaugg ugcgcgagau auucgaaaca cuaaauauca aaaccagucu ccuggacuuu     1620

aacgccacuc uuccgggaaa gucuuucgag cagcagcaug cuuaccaauu guggcaucug     1680

cucuauuccu acgaaggcga uaauagcccc ucggguaaca aaaagcugca ggacuuacug     1740

caggccaaau uuggguucac gaaugcacac ggccaaauuc uugccaacgu cucuuuggag     1800

acagacuaug gaucccucuc agcuaaggcc aucagaaaga ucuuuaccca cauuaaagaa     1860

aaucacuaca gugcagcgug ugcucuggcc ggcuauaacc auagcuccua ccuaucuaag     1920

gaggagaaug aacagaggau acugaaagau cgucucacua uccugccuaa gaacagccuu     1980

cggaauccag ugguugagaa aauuuugaac cagcugauca acuuacucaa uaccauuuuc     2040

gacgaucccu accuggggaa gccugacgaa auccgaauag agcuugcaag agaacugaag     2100

aaaaacgugc aggagaggca acgcuugaau acagagauca ccaagaacaa uacugaacac     2160

gagcggauua caaaaauccu cauuaaggaa gauaacauca aaaauccaac gagaaacgac     2220

auaauuaggu auaagcugua ccaggagcua aagcauaaug gauauaaaac ccuguacuca     2280

aacacuuaua ucccccgcga ggaacucuuu gguaagaauu ucgauauuga gcacaucaua     2340

ccgcagagua uccuguucga cgauuccuuu agcaacaaaa cacuuugcgu ccgaaaggaa     2400

aauauugaca aagccgaucg gaccgcuaug gacuucaucg gcccuuacuu uggggaggau     2460

aaggugcagg aauauauuaa ccguguagag auguugcauc aaaucaaucu gaaaaacccc     2520

gaggaaggaa uaggcaaggg uaaguacaaa aaguuacuca ugaaagccuc ugagauuggg     2580

cagggcuuca ucgaaagaga ccugagggac acacaguaca ucgcaaagaa agcucgccag     2640

augcuucugg agguuacucg gaccgugguc gccacgaccg gaucaauuac aaagagacua     2700

agggaagauu ggggccucgu gaacgucaug caagagcuga auauggacaa guaucgacag     2760

cagggguuga cugagcugau cgagaaaaag gauggaagua ccaaagaacg cauuacagac     2820

uggacuaagc ggaacgauca cagacaccau gcgauggacg ccuuaaccau agcuuuuacg     2880

aaacacagcc auauccaaua ccucaauaac cugaaugcaa ggaaguccga ugacaagaaa     2940

gguaaggaaa uuuucggcau cgagcagaaa gaaacagaga aggugaaaga ugacaccggg     3000

cagacucagc gucgcuuuaa acuuccaaug ccugauuucc ggcaagccgc uaagcaccac     3060

cuggagcauu ugcucauuuc uuuuaaagcc aagaacaaag uuuucacaaa gaauaaaaac     3120

aaguauaaga ccaaaaaugg agaacaggug aagcuggagc uaacucccag aggccagcug     3180

cacaaagaaa ccaucuacgg uaggauaaag aaauauguaa cacaugagga gaaggucucg     3240

agcaaauuua acgcacagcu uauccaaacg guggccaauu ccaaguaccg auaugcgcuc     3300

cugaagcggu uggaagagaa cgggaaugac cccaaaaagg cuuucaccgg caaaaacuca     3360

cuggauaaga cuccauuauu uguugacgcc acacagcagg agaaaauucc uccagugguc     3420

aagcucguga guaucgaaca gggauacacc auucgcaagg acaucacacc ggaaaauuuc     3480

aaagaugcaa agucuauaga gaaaauugua gacgugggca ucagaaaccu gcuucaccau     3540

aggcuggaga cuuacaacgg gaaugaaaag gaggcuuucu auaacuugga uaaaaauccc     3600

aucuggguua acgaaagcaa gggaauuugu auaaagcggg ucaccaucac gggcgugaca     3660

aaugccgagc cucuccacua caaacgagac caccauggua agccaauucu ggauaacaau     3720

gggaacagaa cccccgcaga cuuugugucc acuggaaaua accaccaugc cgcuaucuau     3780

cgcgaugcgg ccggcaaucu acaagacaac gucguuucau ucuacgaagc aguggcuagg     3840

gugaaucagg gucugccuau uaucgauaaa agccuuaaca aggagcucgg cuggcaguuu     3900

cuguucucuu ugaaacagaa ugagugcuuu guauucccaa acgaaggguu ugaccccuua     3960

gagauagauc uguauaaucc ggccaacaac aagcucaucg ccgcuaaucu guuccguguc     4020

caaaacuuuu cccggcuuca guacggaaau aacauuauca gagacuauaa uuucaggcac     4080

auuuacgaaa ccagugugga gaaccgcaag gaacugaaag acaucgcaua caagcagguu     4140

aagagcuugc cuauauuuga gaagauuauc aaagugcgaa ucaaucauau uggccaaauc     4200

gucaagacag gggaguauug a                                               4221


<210>  3305
<211>  4224
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3305
augaucagaa acauucuggg ccucgaccuu ggggugagcu ccauaggaug ggccuacguc       60

caggaggauu cuaaaaauuc agaaaacaau aggaucauua aacugggugu ucgggugaac      120

cccuugaccg uagacgagca auuaaauuuc gaaaagggca aaccuaucac aacuaacgcu      180

gggcgcacgc uggcacgaag ugcccguaga aaucuacaga gguuuaagcu ccggcgcagc      240

aaccugaugg augugcuuaa aaagaauaac auucugaagg agaccgacuu gcucgcggaa      300

gucggcaaaa auuccacauu ccagacucag gagcugagag cuaagucugc caaagagaag      360

aucgaacugu cggaguuugc aagggugcuc uuacugauaa acaaaaagcg gggauauaag      420

ucaagccgca aagccaagaa ugaugaagac ggucaaaucg uugaugggau ggcuguggca      480

aagaaacuuu ucgaggagaa cuugacccca ggagaauaca guuaucagcu gcuacagcag      540

ggcaaaaagc aacucccgga cuuuuaccga uccgaucugc aggccgaguu cgacaaaauu      600

ugggauuuuc agaagcaguu caaucccgaa aucuuuacaa acgagcuuua ugaaaagcug      660

caaggcaaaa acagaaaugc uaccuggaag gaguuggagu ucccuuuuuc ucucgucggg      720

auuaaacaga cuggaacaau gcaggaaaag aaaguggaga aguacuucug gaggagcgaa      780

gccguaaaga aacaacugga cuuugagucc uuagcgguug uguuccagga aaucaacuca      840

aaucugaaca auaguagcgg uuaucucggc gcaauaucug aucgguccaa ggagcuuuac      900

uucuacaacc agaccgucgg ggaguaucug uuucagcaau ugaaaacgaa uccacacacu      960

aagcuaaaga accagguguu cuaccgucag gacuaucugg acgaauuuga gaagauuugg     1020

gaaacccagu caaaguacca uaaagagcuc acaaaggaac ugaaagagga gauccgcgau     1080

auuacuaucu ucuaucaaag aaaacuuaaa agccagaagg gacugaucag uauuugcgaa     1140

uuugagaaua gggaaauaga gaucaccgag aacggcaaga cuaaaaagaa aaccgugggu     1200

uugaaggucg cccccaaauc uuccccucuc uuccaggaau uuaagauuug gcagguucug     1260

aauaacuuac aauuccagaa uaucgagacg aaagaagugu uuccaauaga ccuggauuuc     1320

aagcagagca uuuuugacga gguaaacauc aaggggaaac uaucggcuaa ggaugugcuc     1380

gaccuggucg gauacucugg caaagaaugg aagacuaauu ucaaagagau cgagggcaac     1440

uauacaaacg aaaaucuuua caccgccuuu uugaagauua ucgcauccaa ggagauugaa     1500

uuucccaaag aguucaagau aacuaucgau gacgaaauua aagugucaaa gaucaacgcu     1560

agugccgaga ucauuaaacu guucguucgg gagaaguuca gcgaacucgg gauagauaca     1620

uccauccugg acuuuaaucc ugagcuugaa ggaucugauu ucgagaagca ggcgagcuac     1680

caacuguggc acuugcucua uucauacgaa ggugacgaca guuccagcgg caacgagaaa     1740

cuguaugagu uacuggaaaa uaaguuuggg uucaaaaagg agaacucuaa aauucuagcc     1800

gaaaucggau uuucacagga uuacggcagu cucagcucca aggcaaugcg aaagauuuau     1860

ccauacauca aagagcauaa guauucugug gcuuguucgu acgccgguua caaucacagc     1920

aaaaacuccc ugaccaagga gcagcuugaa aaucgcauau ugaaagagag acuggacauc     1980

cucccgaaga acucacugag gaaucccguc guggagaaaa uuuuaaacca aaugaucaau     2040

cugguaaacu gccuuauuga ugaauauagu gaaaaggaca aggaugggca gaucguuaaa     2100

uacuucaagu uugacgagau acggauugag uuggcucgcg aacucaaacu gaaugccgag     2160

gaaagagcaa cgaugaccuc ugagaucaac aaggccacaa uucagcauca gaaauaugcu     2220

gaagugcuac aaagggaguu cggccugaag gcgccuagcc guaacgauau cauacgguac     2280

aagcucuauc uggagcuugc aaauaacgga uuuaaagacu uguacacuga ugccaagauc     2340

gaacgagaga aucuguucac cgacaaauau gauauugacc acaucauucc acaguccaga     2400

uuuuucgaug acucauucag caacaagguc uuagugccca ggaaugcuaa ccucaaaaag     2460

ggcaauuuua cagccuucga cuaccuggaa aucgagggga aggaaaaacu ugagaaguuu     2520

cugaacauaa uuaaagauuu guacgacaag ggagcaauca cuaaagccaa guaugagaag     2580

cuccagaaaa aggauuucga aaucggugac ggcuuuauug agcgcgaucu gcggaauacc     2640

caguacaucg cuaaaaaguc uaaagaaauu cuauucggga uaacaaacuc cgucgugacc     2700

acaaguggaa gaaucacgga caagcugagg gaggauugga aucucauuaa cacaaugaag     2760

gagcugaauc uugaaaaaua ucgcaaguug ggccugaccg agacuguuau caacagcaaa     2820

ggugaagaga agcaacgaau uaucgacugg accaaacgga augaucaccg ucaucacgcc     2880

auggacgcau uaacaguggc guuuaccacu cauaaccaca uacaguaccu caacuaucug     2940

aaugcuagaa aggaugaaaa acacaaggag caucagaaca ucuucgccau ugagaaucuu     3000

aucacagaaa uuaucgacaa gaaaaacggg ucuaaggaga ggcgcuuuaa agaaccugua     3060

aagaaucugc gggcagaggc cagaaaacac cuagaugaaa uacucauuuc ccauaagguc     3120

aagaacaaag ugguuacgaa gaauaucaac aaaaucaaga aaaagggcuc agugauugcu     3180

aagaccgagc ugacuccaag gggacaguug cacaaagaga caauauacgg caguagcaag     3240

uuccugaaaa ccaaggaaga gaaaaucucg gggaaguuug acgucgaaac aauucaaaag     3300

gugcagaaug agcgauaucg caacgccuua cucaaacggc ugcaggaguu cggaggugac     3360

uccaagaaag cauucacugg caagaaugua aucucuaaaa accccauuuu ucuuaauaac     3420

gaaaagaccg agcaacugcc ggaaacggug accuuggcuu gguacgagau gggguacaca     3480

aucagaaaag ccguuaaucc ugauaacuuc aaggacuuua agaauauaga aaagacuauc     3540

gauaagggaa uuagggacau ccucaccaaa cgucugaagg aguucaacgg caacagcaaa     3600

gaggcguuuu cagaucuaga caagaauccc auuuggcuga acaagaguaa agguaucucc     3660

auaaaggccg ucacaauuac ugggaucaau aacgcugaag cacuucauua uaaaaaggau     3720

caccucggca aagacuuccu ggaugagaau ggaaagcgca ucgccgugga cuuugugucu     3780

accggcaaca aucaccaugu cgcuauuuac gaagaugaga acgggaauuu gcaggaaaag     3840

guugugagcu ucuaugaggc cguggagcgg guaaaccaga aucugccaau cauagacaaa     3900

gaauacaacu ccgagucagg auggaaguuu uuauucacga ugaaacagaa ugaaauguuu     3960

cucuucccua gcgaggacuu uaaccccaag gaaauggauc uguucgacga gaaaaauguc     4020

aaccuuauua guaagaaccu guucagagug caaaaguuua caaucaaaga uuauuucuuu     4080

aggcaccauu uggagaccaa uguugaagac ucuuccccac ucaagggcau uacuuggaaa     4140

cgagaggguc ugaacgggau cgaaaauaua cuaaaggugc ggcugaacca ccuuggaaaa     4200

auuguccaga ucggcgagua cuga                                            4224


<210>  3306
<211>  4227
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3306
augaccaaga acauccuggg ccucgacgug ggggucagcu ccauuggaug ggcccuuaua       60

aaugaggaua ucgaaaaccc caaaaauaac aagauuauca aacugggugu uagagugaau      120

ccuuugacag uagacgagca gauuaacuuc gaaaagggca aaccaaucac uacgaaugcu      180

gggaggaccu uagcacgggg agcccgccga aaccugcaac guuuuaagcu aagaagguuc      240

caccucauag auauccugca gaagaauaac auucuuaaac aggcggaccu guuggcugag      300

gugggcaaaa auucuacauu ucagacucaa gaaauccggg ccaaaucagc aaagcagaaa      360

gucgagcuca gugagcuggc ccgcauuuuc cugcucauca acaagaagag agguuacaaa      420

agcuccagga aggcuaaaaa ugaagaugag gggcagauaa uugacggaau guuuaucgca      480

aagaaauuau augaugaaaa gcugaccccg ggcgaguacu cuuaucagcu uuugaaagag      540

ucgaagaagc aacugcccga cuucuaccgg ucagaucuac agagcgaauu ugacaaaauu      600

uggaacuucc agaagcaguu uuauccugag aauuucagug auaaccucua caaagaacug      660

caaggcaaga acaaaaaugc cacauggaag acccuugaga ucccauuuuc ccuggugggg      720

aucaagcaga cuggaacauu gcaggaaaag aagcucgaga aauauucuug gcgcagcgac      780

gcuguuaaga aacaacugga uuacgagaag uuagccgugg ucuuccagga aauuaacucc      840

aauauauaca acucaagugg uuaucugggc gcgaucagcg accgaucuaa ggagcucuac      900

uuuaauaacc agaccguggg ggaauaucuu uacaaucaga uuaaagagaa cccccauacg      960

aagcugaaaa aucaaguauu cuauagacag gacuacuugg augaguucga aaagaucugg     1020

gagacucagu ccaaauauca caccacacua acuaaggaac ugaaggagga aauaagggac     1080

auuaucaucu uuuaccagcg gaaacucaag ucacaaaaag gacugauuag caucugcgag     1140

uucgaaaacc gugagauuga gauaaaggaa aauggcaaaa ucaagaaaaa gaccguuggu     1200

cuuaaggugg caccuaaaag uucuccacug uuucaggagu ucaagauuug gcagaucuug     1260

aacaaucucc aguuucaaaa caucgaaucc aaagagauuu uccccauaga ucuggacuuu     1320

aaggagagca ucuucaauga agucaacauu aaagggcgca ucucggccaa ggcuguguua     1380

gaucugcuag gauacucugg caaggagugg aacccuaauu uuaaagacau ugaaggcaac     1440

aauacaaacg agaaucucua uaacgccuac cugaagauca uagcagauga agaguucgag     1500

cuuccaaaag aauuuaaguu gaauggggac gaugaccugu auguguccaa aaucgcuugu     1560

ucagccagua agauuaagga uuucgucaaa gagaaguuua gcgaaaucgg aauuaacacc     1620

uccauccucg acuucaaucc ggagcuggaa gguaaagacu ucgagaagca ggcgucuuac     1680

caguuuuggc aucuucugua uagcuacgag ggcgaugacu cacagagugg gaacgaaaaa     1740

uuguaugagc uccuggaaaa gaaguucgga uuuaaaaagg agcacuccaa aauauuaagc     1800

aauauuggcu ucccccugga uuacgguaac cuaucuacga aggccaugag aaaaaucauu     1860

ccucaucuca aggcagggcu gucauuugcu aaaaagagua aggaagacaa uauccuuagc     1920

gccacugcua uggccggcua caaccacucc aauucuuaua caaaagagga uuuggcaaag     1980

aggguucuga aagagaagcu cgaaguguuc cccaaaaacu cgcugcggaa cccaguagug     2040

gagaagauau uaaaucaaau gaucaaccug gucaaugaag ccguggagga guacggaacc     2100

auugacgaag uucgaaucga gcuugcucgc gaauugaaga aaaacgcgga ggaaagagca     2160

aauaugacua gcgagauugg caaggccaca cuccugcacc agaaauaugc ugagauccua     2220

aagaaagaau uugauauacc ugugccaucc aggaacgaca uuauccggua caagcuguau     2280

cucgagcugg ccaauaacgg guacaaggau cuuuauaccg acguccagau caaacgcgaa     2340

gaguuguuca cgaauaagua cgauauugac cauaucauuc cccaaucaag auuuuucgau     2400

gacaguuuuu cuaacaaagu gcugguaccg aggggagcaa auuuaaagaa agccaaccag     2460

accgcuuucg acuaccucga aaaugaggau aagcagaagc uggagaaauu ucuuaacaua     2520

aucaagaauc uguaugacaa aggugaaauu acaaaggcca aauacgagaa guugcagaag     2580

aaaggcagcg auaucgggga aggauucauu gagcgugacc uccgggauac ucaauauauc     2640

gcaaagaaag cgaaggaaau acuguucggc aucacccacu ccguuauuuc aacaagcggu     2700

aaaaucacug acaagcuacg agaggauugg aaccugguga acaccaugaa agagcucaau     2760

cuggacaagu acaagaaacu uggguugaca gaaacccugu uaaacucuaa gggcgaggaa     2820

aaagagagaa uuaucgauug guccaagagg aaugaccauc gccaccacgc uauggaugcc     2880

cucacugucg cauuuacgac acauaaccac auacaguauc ugaauuaccu uaacgcccgg     2940

aaagacgagg aucauaagca gcacaaggug auuaguaaua ucgaaaaccu gaucaccaaa     3000

gucuucgaga agaaaaaugg agcuaagcag agaagguuua uugaaccuau aaacaauuuc     3060

cgcguggacg ccaaaaagca ucuagacgag auccucauua gccacaaggc uaaaaacaag     3120

guugugacua aaaauaucaa caagauuaaa aagaagggcu cuguagucgc aaaaaccgaa     3180

cugacacccc gagggcaauu gcacaaggag accaucuaug gauccucaaa auuucugaag     3240

acuaaagaug agaagauaag ugguaaauuc gacuuagaaa caaucaauaa ggugcagaac     3300

gagaaguaca aaaaugcccu ccugcagcgg cuucgugaau uuggcgagga uagcaagaaa     3360

gcguucacgg ggaagaacgu uauuucgaaa aacccaaucu accuggacga agagaagacc     3420

uugcagauuc cugaguccgu gacucucgcc ugguaugaaa ugaucuacac aauaagaaag     3480

cccgucaauc cagauaacuu uaaagacuau aagaauauug auaaagugac cgacgagaag     3540

aucagggcug uacugacaga ucgccuacgg aaauucaacg gaaauucuaa ggaagcauuu     3600

agcgaccugg agaagaaccc caucuggcuu aauaaaucaa agggcauugc caucaaaacu     3660

gugaccauaa cggguaugag uaacguugaa gcucuccauu acaagaaaga ucaccugggg     3720

aaggagauuu uggacaagaa aggcgacaga augccugucg auuucguguc caccggaaau     3780

aaccaucacg ccgcaaucua ugaggacgcg aagggcaauc ugcaagaaag gguggucucu     3840

uucuacgagg ccguugaacg agugaaccag ggguuaagcg ugguaaauaa agauuauaac     3900

gcuggacucg gcuggaaguu ucuguucaca augaaacaga augagauguu ucuuuuccca     3960

uccgaggacu uuaacccgaa ggaaauugau cuguacgacg agaaaaacuu gaagcucauc     4020

gccaagaauc uguuccgcgu ccaaaaauuu ggugaacuau caaagagcgg guucugguuu     4080

cggcaccauc uggagaccag uguggauguu aaaaaggaac uuagaaacua cucuuaucuc     4140

gacuucuacu ccaaagauuu uaugaagacu auagugaagg ucaggcugaa ucacuuggga     4200

aaaauugugc agguuggcga guauuga                                         4227


<210>  3307
<211>  4230
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3307
augagcauca uuaacuucca gagaaggggc cugauggaga cccaagccuc caaucagcuc       60

auaucuucac accuuaaggg guaccccauc aaagacuauu uugugggacu ggauauuggu      120

acaaguagcg ucggcugggc uguuacuaac aaggcauacg aauuguuaaa auuccggucc      180

cauaagaugu gggggucucg ccuguuugac gagggagaau cggccguggc gcgacguggc      240

uucagaucaa ugaggcggcg ccuagagaga aggaaacucc ggcugaaacu ucuggaagag      300

uuguuugcug augccauggc acagguagac ccuacguucu uuaugcgccu ccgagagagc      360

aaguaucacu acgaagauaa aaccacaggu cauaguucca agcacauccu guucauugac      420

aaaaauuaua cugaucagga cuacuauaag gccuacccaa ccaucuacca ucugagagcu      480

gagcucauga aagaugggac agacgauaua aggaaguuau uucuggcagu gcaccacauc      540

cuuaaguauc ggggaaacuu cuuguacgaa ggcgccaccu uugacucuaa ugcuagcacu      600

cuggaugacg ucauuaaaca agcccuagag aacaucacau ucaauugcuu ugacuguaac      660

uccgcgauuu caaguaucgg ccagauacuc auggaggcag ggaagaccaa aagcgauaag      720

gccaaagcua uugaacaucu gguggacacg uauaucgcca cugauaccgu ugacacaucu      780

uccaagacuc agaaagauca ggugaaggag gacaagaaac gucuuaaggc auucgcuaau      840

cuggucuugg gacucaacgc cucacugauu gauuuauucg guagcgugga agaacuggaa      900

gaggaccuca aaaagcugca aaucaccggc gauacauacg acgauaaacg cgacgagcuu      960

gcgaaggccu ggagugacga aaucuauauu auagaugacu gcaagucugu auacgaugca     1020

aucauuuugc uauccaucaa agagccgggg cugaccauaa gcgaaucgaa gguuaaggcu     1080

uuuaauaagc acaaagacga ucucgccauu cugaagucuc uucugaaguc cgacagauca     1140

aucuauaaca cgauguucaa aguggaugag aagggauugc auaauuacgu ccacuacauc     1200

aaacagggca ggacugaaga gacaaguugu aaccgggagg acuuuuauaa guacaccaaa     1260

aagguggugg aaggucuccc cgauagcaag gacaaagagu auauucuguc cgaaaucgag     1320

uuacagacuc ugcuaccucu ccagcgaauu aaggauaaug gggucauacc auaccaacug     1380

caccuugaag aguugaaagu uauccuggcu aagugcggac ccaaauuccc uuuucucaac     1440

gagguggccg acggcuucuc uguagcagaa aagcugauua aaaugcuuga guuucgcauc     1500

ccauauuacg ugggcccccu gaacacacau cacaaugucg auaacggggg auucgccugg     1560

gcugugagaa aggcgagcgg uaggguuacc ccuuggaauu uugacgacaa gaucgaucgg     1620

gaaaaaucag cagccgcuuu cauuaagaac uugacgaaua aauguaccua ucuccugggc     1680

gaggacgugu uaccaaagag uucccugcua uacagcgagu uuaugcuccu gaacgaacuu     1740

aauaacgucc gcauagaugg gaaaccguug gagaaggccg ugaaggaaca ucugaucgag     1800

gcaguauuca aacaggacca caagaaaaug acaaagaaua gaauugaaca guuucucaaa     1860

gauaacaauu acauccccga gaagcauaag ccugagauua cuggacugga cggcgaaauc     1920

aaaaacgauu uagccucuua uagggacaug guucguauac uggguaauaa cuucgaucuu     1980

ucaauggcug aggaaaucau uaccgacauc acaauuuucg gggagaguaa gaaaauguug     2040

cgggaaacuc uccgaaauaa guuuagcucc agacuggaug acgaggccau caaaaagcua     2100

ucuaagcuga gguaccgcga uuggggccgg cucucgagaa aacugcuuaa cgugauagag     2160

ggaugcgaca aggcaggcga cgggacccca gaaacaauua ucauuuugau gaggaacuuc     2220

agcuauaauc ugauggaguu acucggagau aaauuuuccu ucauggaacg cauccaagag     2280

auaaacgcga agcugaucga aggucagauu gucaaucccc acgacaucau ugaugaccuu     2340

gcucugagcc cugccgugaa acgagcaguc uggcaggccu ugcggaucgu ggaugagaua     2400

guucacauua agaaaacccu cccagcucgu aucuuugugg agguaacuag aaguaacaag     2460

gccgaaaaga aaaagaaaga cucuaggcag aagcgccuga gcgaucuaua cgcagcuauc     2520

aaaaaggacg augcccugcu cuccggccug acgaaugagg aguucggggg acuuaaguca     2580

ggcuuggcga aauaugacga ugccgcucug cggagcaaga aauuauaccu cuauuacaca     2640

caaaugggua gaugugcaua caccggggag gucauugacc ugucucagcu uaacacugac     2700

aauuaugaua ucgaccauau uuaccccagg ucccugacca aggaugacag uuuugauaac     2760

cuagugcucu gcaaacgaac agccaaugcu cagaagucag acaccuaucc gauaucuaag     2820

gagauccaga aagcccaaaa ggcauucugg auuuuucuga aacagcaggg cuugaucucc     2880

gaacgcaagu acgagcggcu gacuagaauu acaccuuuaa cggcggauga ccucucagga     2940

uucaucgcca ggcagcuggu ugaaaccaac caaaguguga aagcugccac uacacuucug     3000

cgucgcuugu aucccggcgu cgauguggua uuugugaagg cagagaaugu uaccgacuuc     3060

cggcacgaua acgacuuuau aaaggucaga agccucaauc aucaccacca ugcuaaagau     3120

gccuaccuga acaucguggu ggggaauguc uaucacgaaa gguucacacg aaacuuccgg     3180

gcauuuuuca agaaaaacgg agccaaucgc acuuacaacc uagcuaagau guuuaauuac     3240

gacguuaacu guaccaaugc gaaagacggu aaggccuggg augugaagac gucgauggac     3300

accgugaaaa agaugauggc auccaacgau guaagaguca caaaaaggcu gcuugagcag     3360

ucuggcgcuc ucgccgacgc cacuguguau aaggcugcag uugccgagaa agcgaaggau     3420

ggggcuuaca uuggaaugaa aaccaagagc ucaguguucg ccgacgucag uaaguauggc     3480

gguaugacaa aaaucaagaa ugcauacucc auuaucgugc aguaugccgg gaaaaagggc     3540

gaaguuguga aagagauagu accacugccu auuuacuuga cuaaccggaa uaccacggau     3600

caggaccuga ucgauuaugu cgcuucuauc auuccccaag caaaggacau aagcaucauu     3660

uaccgaaagu uaugcaucaa ccagcucgug aaagugaaug gauuuuacua uuaccugggc     3720

gggaagacaa acuccaaauu cuguauugau aaugccaucc aggucauagu uucaaacgaa     3780

uggaucccau aucuuaaggu gcuggagaaa uuuaauaaca ugagaaagga caacaaggau     3840

uugaaagcga auguaauuag cacccgcgcu cucgacaaca agcauacuau cgaagugagg     3900

auugucgagg agaaaaauau cgaguucuuu gacuaccugg ugaguaagcu aaaaaugccc     3960

auauaucaaa agaugaaggg aaacaaagcc gcagaacugu cugaaaaggg cuacggucuu     4020

uucaaaaaga ugucccucga ggaacagagc auucaccuga ucgaguuguu aaaucugcuc     4080

acaaaccaga aaaccacuuu ugagguuaag ccucugggga ucaccgccuc gcguucaaca     4140

gugggaucua aaauuaguaa ucaggaugag uucaagguca ucaacgaauc cauaacgggc     4200

cuuuauagca augaagugac cauuguuuga                                      4230


<210>  3308
<211>  4233
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3308
augaccaaga acauccuggg ccucgaccuu ggggugagcu ccauuggaug ggcccugguc       60

caggaaaauu cuaaaaaccc cgaaaauaac aagauaguua aauugggugu gagaguaaau      120

ccuuuaacag uggaugagca aaucaacuuc gaaaagggca aaccaauuac uacgaaugcu      180

ucaaggaccc uggcacggag ugcccgccga aaccuacagc guuuuaagcu cagaagggcg      240

aaucugaucg acguccuuaa gaaagggaac auucugaagc agggagauuu gcucacagag      300

aucggcaaaa auagcacuuu ccagacccaa gaacugcggg cuaaguccgc caaagagaag      360

guggagcugu cugacuuugu ucgcauacuc uuacugauca acaagaaaag agguuacaag      420

ucgucaagga aagcaaagaa ugaagaugag gggcagauua ucgacggaau ggccguggcu      480

aaaaaguugu augaagagaa cauuacaccg ggcgaguaca gcuauaaacu ucugcaggaa      540

ggccggaagc agcuacccga uuucuaccgc agugaccucc aagcagaguu ugauaagauc      600

uggaauuucc agaaacaguu uaacccugaa auauucacca acgagcugua uaagcgacuu      660

caggggaaaa auagaaacgc cacuuggaag gaacuggaga uuccauuuua cuuggucgga      720

aucaaacaaa uugguacaau gcaggagaag aagaucgaaa gguauuucug gcgguccgag      780

gcugugaaaa agcagcucga cuuugaaucu cuggccguag uuuuccaaga gaucaauagc      840

aacuuaaaua acuccucagg cuaccugggg gcgauuagug aucguagcaa agaacucuac      900

uuuaauaacc agaccguggg agaguaucuu uacaaucagc ugaagauaaa cccccacacg      960

aaauugaaga aucaggucuu cuaucgccaa gacuaccuag augaguucga aaagaucugg     1020

gagacucagg caaaauauca uaagaaacug accaaggaac ucaaagagga aauuagagac     1080

aucgugauau uuuaccagag gaagcugaag ucucagaaag gccuuauuuc cuucugcgag     1140

uuugagaacc gggaaaucga gaucacagug aaugguaaga cuaaaaagaa aaccgucggg     1200

cugaagguug ugccucgauc aagcccauug uuccaagaau uuaaaauuug gcagcgccuc     1260

aacgaccugg aguucaagaa uguagagaca aaggaaaucu uucccuuaga gcuggaacac     1320

aaagagauuc uauucggaaa ccucaacaua aagggcaauc ugaguucuaa agaacuuauc     1380

gagcaguugg gcuauccuuc caaggagugg aaauguaacu uuaaggaaau ugaugggaau     1440

aagacccaug agaaccugua cacggccuuc cucaaaauca ucgcuaauga aggaauugag     1500

uuuccaaagg aguucaaacu gagcauagac gaugacauca aggugucgaa aauugauguc     1560

ucuuccgaga agaucaagga guuuauuaaa gaaaacuucc uuaagcuggg uaucaauacu     1620

ucaauauugg acuucaaccc ggagcucgau ggcaaagacu uugaaaagca ggagaguuac     1680

caacuguggc acuuacugua uagcuacgaa ggggaugacu ccgccucugg aaaugagaaa     1740

cuauaugagc uccuggaaaa gaaguucggc uuuaaaagag aguacagcaa gauccuugca     1800

gauguggguu ucucacagga cuaugggagu uuguccagca aagcuaugag gaagauuuuu     1860

acauacauca aagaaaacaa guauucugag gccugccauc uggcgggcua caaucacagu     1920

aaaaacaguc ucaccaagga gcagcuggcc aaucggauuu uaaaggaaca gcuggagauc     1980

cuucccaaaa acgcauugcg caauccuguu guggaaaaga uacucaacca aaugauuaac     2040

cuggucaaug aggugaucaa agaauacgga aagccagacg agauuagaau cgagcuagcu     2100

agggaacuga aaaagaacgc cgaggaacgu gcuaauauga cuagcgauau auccaaggcc     2160

acacuccugc accagaaaua ugcagaggua cuucagaagg aguucggcau caaagccccc     2220

ucucggaacg acauuauccg auacaaguug uaucuggaau uagcuaauaa cggguacaaa     2280

gaucucuaua ccaaucaaaa gauugagaag gaaaacaucu uuacggacaa auacgauaua     2340

gaccauauua ucccucaguc gagauucuuu gaugacagcu ucuccaauaa gguucuggug     2400

ccaaggugug cgaaccagga uaaagacaau uauaccgcag ucgauuacau cgagaagaaa     2460

uacggagaac gcgguaagga ggacuuucuu aaccggauuc accugguguu caagucaaaa     2520

gaagccaucg cuggcaagag ugagucucag auuagcucca ugaauacaaa auuuuugaag     2580

cuccagaaaa aggggucaga gauaggagaa ggcuucaucg agagagaucu gagggacacu     2640

caauauauug ccaagaaagc aaaggaaauc cuauucggua uuaccaacag cgucaucucu     2700

acauccgggc gcauaacuga caaacugcga gaggauugga aucucgugaa caccaugaag     2760

gagcugaacc uugaaaaaua ccggaaguug ggccugacag aguuaaucgc caauaguaaa     2820

ggagaagaga aggaacguau uaucgacugg accaagagaa acgaucauag gcaccacgcu     2880

auggacgccc ucacuguugc auuuacgaca cauaaucaca uucaguaucu gaacuaccuu     2940

aaugcgcgca aagaugagaa gcauaaagag caccauaaca ucuucgcuau agaaaauauu     3000

aucaccgaga ucguggaaaa gaaaaacggc agcaaggagc ggagauuuaa gcugcccauu     3060

aaaaauuuca ggacugaagc caagaaacag cuagaggagg uacucauauc ucacaaggcc     3120

aaaaacaagg ucgugaccaa gaauaucaac aaaacaaaga aaaagggagg aguugugucc     3180

aaaauugucc ugaccccgcg aggucaauug cacaaggaaa cuaucuaugg cucagcaaag     3240

uuucugaaaa caaaggagga gaaaauuagu gggaaguucg acuuagaaac gaucaauaaa     3300

gugcagaacg aaaaguuuaa aaaugcucuc cuggaucgcc uuaaggaguu caacggagac     3360

agcaagaaag ccuuuaccgg caagaaugua ccugcuaaaa accccauaua ccugaacgag     3420

cagaagacug aacaggugcc agagucgguu gucuuggcau gguaugaaac agguuacacc     3480

auccggaaag ccgugaaugc ggauaacuuc aaggacuaca agaaucucga gaaaauuauc     3540

gauaaggggg ugaaagagau ucugaagaac agacuaaaug ccuuuaacgg caaugcuaaa     3600

gaagcauucu ccgaccugga gaagaagccu aucuggcuua accaaucuaa aggaauagcc     3660

gucaagacag uuacuauuac cggcaucaau aacgcugaaa cgcuccauua uaaaaaggau     3720

cacuucggga augagcuguu ggacgaaaac gggaaaaaga uccccgugga cuuugugagc     3780

accggcaaua accaucacgu agccauuuac gaggaugcag acgguaaucu gcaggagagg     3840

gucgugucau ucuaugaagc gguugagcgu gugaaccagg gguuaaguau cauagauaag     3900

gaauacaacc aagaccucgg auggaaauuu cuguucacaa ugaagcagaa ugagauguuu     3960

cuuuucccau ccgaagauuu uaaccccaaa gagacugacc uguucgauga gaagaauuug     4020

aaccucauuu cuaaaaauau guuucgcguc cagaaguucg gcagccugcu auccgguuuu     4080

ugguuccggc accaucugga aaccacagug gaccuuaacu caagccucaa agauacuuca     4140

uauaagguua uccagucuug caauaaccug aaguugauug ugaaaguaag auuaaaucac     4200

cugggcaaga ucguccaaau aggggaguac uga                                  4233


<210>  3309
<211>  4236
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3309
auggugaaga aagaguacuu caucggccug gacaugggga ccagcuccgu cggaugggcc       60

guugcugaua caaacuauaa ucuccuuaga gcaaagggua aagacaugug gggcgugagg      120

cuguuucccg aagccucuac ugcggcugau cggcgcucac accgaacggc ccguagaaac      180

aauaagagga aagcagcccg ggagggguuc uugcgcgaaa uuuuugagga cgaaauaaac      240

aagaucgauc cuggauucuu ugagagauua aaggagagua aauacuaucu ggaagacaag      300

cuaaccgaua caccauucgu acucuuugcu gacaaaggcu acacugauaa ggaguauuac      360

gacgaauauc cgaccguguu ccaucugauu aaagagcuua ucagcgagaa gggucuggaa      420

ccccacgaua uuagguuggu cuaccucgca ugccugaaua ucuacaagca ucgggggcac      480

uuucugaacg acacacucuc cggagauggc auagacaaua ucaacucuuu auguaaugac      540

uucaccucgu aucuggccga uuacaaccaa aauucagagg acgauggcac ugagaaaaug      600

gaccuugaua gcacaguguu ggcugggguu uuugaggaaa cccugugcaa caagcaucua      660

aguaauuccg ccaaaaagcg cgaccucauu gauaaacugg gaaucacgaa caagacuaaa      720

uaucuuaccg agaugauggc gcugauuucu gguuuggcaa gcuccguguc aaauauguuc      780

gccaaggaca cauacgauga ggaacagaag aaauuuaagc ucaguuuccg aagcucuaac      840

cuggacgagg auauggucaa agugcaggcu auguuaacuc cugaagagua ugaaaugauc      900

uguauaauga agaaaauuca cgacuggucc guaguuucag ugaucauggg cagccacaag      960

uacauuagug acgccagagu cgagcuguau gagaagcaua aacaggaucu cgcaaaacuu     1020

aaagcuaucu acaaggaaua ccugccagac aaauaugaug ccauguucag ggagaugacc     1080

gacaacucuu acuccgcgua uguggggaag gugagcucga auaagaaauc ucagacagga     1140

aaccacaagg uccggaugca acgucgcggc gccaaaugca agcaggaaga uuuuuacgac     1200

agaaucaaaa aggagauuaa uuccguuccc caggaagaga agauagauau cuugcaggac     1260

auggagacca acucauuccu accuaaacaa cugacgaaug aaaacggugu gauuccauau     1320

cagcuccauc uggcagagcu uaagaaaauc cuggauaaug cuaguacuua cuugcccuuu     1380

cucaacagcg ccgacgaaac agggauaucc aauaaggaua aaauuaucca gcuguucgag     1440

uuuaggaucc cuuauuacgu aggaccauua uucaagucug agaaaaacaa gaccaaugcu     1500

uggguggucc ggaaggaaag cggcaaagug uacccgugga acuuuaauga gaagauugac     1560

gaaaaacagu cagccgagau cuucauugaa cgaaugguuc gccacuguac uuaucugaac     1620

aaugagagug cacuacccaa gaacucccuc aaauacgaga gauuuagggu gcugaacgaa     1680

cuuaauaacu ugcggauaaa uggcgagcgc aucagcccug aacugaagca aagaauuuau     1740

aacgaacucu ucaaggucaa aaagaaaagg aucacaaagu cuaugcugug cgaauaccuu     1800

uauaaaaaug ggaucgccga uaaggacuca gagcugagug gauuugacgg uaccuucgcu     1860

aacgcguugc ucagcuacgg caaguuucgu gagcuguuca auguggauac guuaaccgac     1920

aaacaggaag cagccgcuga ggauauuaua aaguggucca caguauaugg ggacucuaaa     1980

aaguuccugc aggagaaaau cgaagaaaac uacggaccag agucggagaa uccccuacuc     2040

gauaacaagc aaauuaagcg gguuaugggc uacaaauuua cugacugggg uaaucugagc     2100

cgagaguucc uuaacuugga aggggccgau aaggacaaug auaccacaac uggcgaagug     2160

augccucuga ucaccagaau gugggaggag aacuauaauc ucauggaacu gauguccaac     2220

aaauuuucau acagggacgc auuagccgag aagacagaag agauugagaa aacccugacu     2280

gauaucgaau augaggaccu ugaagauuug uacauaagug cuccagucaa gcgcaugacg     2340

uggcagacac uccugauccu aaaagagauu uaucagauca ugggauacgc ccccaagaag     2400

auuuucgugg aaauggcacg ggaggaagag gagaaaguca acggcaaggg ggcgagaaaa     2460

ucuagcagga agaaacgccu ggaagaccuc uaucgaaauu guaaggacgc uuccaaggag     2520

cugucaaccg gaaucaaaaa cgccgaugaa gcaauacuuc ggcguaagaa auuguaccug     2580

uacuauuuac agaaggguaa augcauguac agcggcaaga cuauugacuu uaagucucuc     2640

uucaccaaua acuaugauau cgaccacauu uacccgcaau ccgugguuaa agaugacagu     2700

cuggagaaua acauggugcu uguagaaaga aauuauaaca auaagaaaag cgauacauuu     2760

ccuaucuucu cugacuggca gaacaagaug aaacccuuuu ggaccaugcu gaaggagcag     2820

ggguucauaa augaggaaaa guacaaaagg uugacucgca cagaugagcu cacggacgau     2880

gaacuggcca acuuugucaa ucggcagcua guggagacca gacaagcuac uaagguuauc     2940

gccgagcugc ucaaaaacuc cuucgacgac auugauucaa agaucgugua ugcaaaagcu     3000

ggaaauguca gugaauuuag gcauaaguuc agcaugaagu acgacaaaga gacagaaaag     3060

ucguccguga uucacccaga gcugguaaaa ugucgaauca uaaacgauuu ccaucacgcc     3120

aacgacucuu accuuaauau ugugguuggc aacaucuaug augucaaguu uacccagaau     3180

ccuuacaacu auaucaaaga auacaagaaa cagaagaaua acagcgcguu gucagagcag     3240

gagaaggaaa aauaucauau ggacaagauu uucaauuuug augugaaacg caacggugag     3300

guggccugga ucaguagagg ggacaauaag acacuggaaa cugucuuaaa aguuaugcgg     3360

aagaacaccc ccauugugac gaggaugaau uucgaggaac acggccaacu cgcugaucug     3420

accauauacu ccgcaucuga cgcccuuagc aagaaaggag ugggcuauau caagguaaag     3480

gcuuccgaug cccgucugga cguccuaaag uacgggggau acaaaaagua uaagggugca     3540

uacuuuuucc ucguggagca cacagagaaa aacaagcgcg uucggacuau ugaaaauaug     3600

ccacuguauu ugaaaaacga gcugaauacc aaggagaaaa ucgaauuaug gugcgcggau     3660

aaggaaaacg gccucgggcu gguggacccc gacgucagac uuggaaagau uaaacuguac     3720

ucaaagauca ggauagaugg cuuugacuug uaucucacag gucgaacugg gaauaaccug     3780

cuaaccagca acgccgugca gcugaaaauc ggcaguaaua agccuucuga uaacgacaaa     3840

cuuuucuggc gguacuauau uaauaagcuc uccaaauaca cggagaacag cgaguuuguu     3900

ucgauccaga auaacauuaa gcuguacgau uuguucgugg aaaucaauaa cgcuggagua     3960

uauucaaagc gccagaaucc aauaggcgac aaacuguuag aggggagauc uaaguuuauu     4020

aaccucacac ugaguaaaca agucgaaacc cuucugggaa ucaucaggau uuacuccuug     4080

gagaaucagg gcgccgaucu cagcaacaua ggugggcagg agaagaaugg aacuaugaaa     4140

ccgcauaagc ggaucucuga caagaaagaa gugcugcuaa uuaaccaaag cgugacaggc     4200

cuguaugagu ccaagaucga ucuucucaaa auuuga                               4236


<210>  3310
<211>  4236
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3310
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugggc ccugauaaaa       60

cagaacgcug aggaaagaca agguuccauc uugggcaugg ggacaagggu guuacccaug      120

ucucaggaug uccugggaga cuucggcaag gguaauucag uuagucagac ugcagcccgg      180

acgggguacc gcggcauucg acgucuaaga gagagguuuc uccugcggcg cgaaagacuu      240

cacagggugc ugaacaucuu gggauuccuc ccuuauuacu augcggcuca gauugauuuu      300

gacaaacggu ucggcaaguu uaaagccgag accgaaccaa agcugccgua caauaacgag      360

ggguucaucu uuaagaauag cuucgaggaa augcuguccg auuuuaaaau aucucaaccc      420

gagcucuuau cgggaaacaa gaaaguaccu uaugacugga caaucuacua ucugcgcaag      480

aaagcagugu cacagaagau ugaaaaggag gagcuugccu ggaucuugcu gaauuucaac      540

cagaaacgag guuacuacca gcuaagaggc gaagauuuug acgaggaaaa ggauaaaacu      600

uucgucaccc ucaaugugga caagguuauu gauagcgggg agaaagugaa gggaaacauc      660

cuguuugaca uauauuucac aaauggcugg aaauacgaaa agcaaaccac uaagccagag      720

gauuggcuug guaggaguaa agaguucauu aucacagaau cccuguugaa gaacggggag      780

guccggcgua ccuuuaaagc uguggacucu gaaaaggacu ggauugcaau caaagccaag      840

acggagcagg aaaucgagac ugcucauaag accguaggaa gcuauauuua cgaugccaua      900

cuccagaauc ccaaacagaa gaucaaaggc aagcugguuc gcacaauuga gagaaaauuc      960

uauaaggaag aguuaaggaa gauccuggaa aaacaaaagg acuuucaccu cgagcuucag     1020

aacgaagauc uguacuccga cugcgugcgg gaguuguauc gaaacaauga uacucaucag     1080

cuaucacugg cgaaaaagag uuucauacac cucuuucugg aggacauuau cuucuaccaa     1140

cgcccucuua aaagccagaa gucuuccguc ucaaacugua cccuggaaug cagaaaguac     1200

agggauaaaa auggcgcaga ggggauccag uuuuugaagg ugauuccaaa aagcaacccc     1260

uauuaccagg aguuccggau cuggcaaugg augaguaauc ucaagauuua ucgcagagac     1320

gaugacguga acauaacauc ugaaugucug aaaaccgccg aggauuacga agacuuauuu     1380

gucuuccuga auaucaggaa ggagguugac cagaaagcau gccuacguca uuuuaagcuc     1440

uccgaaaaga cgcaccggug gaacuaugug gaggauaaaa aguacccuug uaaugaaacu     1500

ggaacaauga uuagcgagcg acugaaaaag guaggugacu cgccagauaa caucuucacc     1560

agagagaugg aacagcagau cuggcacauu auauauucug ugaaugacaa aacugaguac     1620

gaaaaggccc uuaaguccuu ugcuaaaaag aacgaguugg auaaugacuc auucuucgag     1680

gcuuuuagga aauucccgcc cuuugauagu gaauacggca gcuucuccga gaaggccauc     1740

aaaaagcugc ucccucugau gcgcauuggg aagcauugga acugggaagc ggucgacgag     1800

aaaaucaaga aucggauuca gaaaaucaua aucggagaau augaugagaa cauuaaggac     1860

aacaucagag agaaaacacu uucucugcaa aaggaaagcg auuuucaggg cuugccacuc     1920

uggcuggccc aguacguggu uuaugguagg cacucagagg caaguguggc ugggaagugg     1980

aacuccgucg acgauauuga agaguacuua aaagaguuca agcaacauag ccugcgcaau     2040

cccaucgugg aacagguaau aauggagacc guucgagugg ucaaagacau uuggaucaag     2100

uauggcaaug gagccgcuga cuuuuucgau gaaauucaca ucgagcuagg ccgggaaaug     2160

aaacucccug ccaaggagcg uaacaaaaug acgcagaaga uaucugaaaa ugagaacacc     2220

aaucugagaa ucaaggcacu uuuggccgag augaugaacg acucagaagu gcagaauguc     2280

aggccauaca gucccaugca acaggagauu cugaaaaucu augaagaugg ggcucucaac     2340

agcgagauuu ccguggacga ugacauccug aagauaucua aaacagcgca gccgucgccu     2400

agcgauuuaa agaaauacaa gcuguggcuu gaacaaaagu accgcucccc cuauacugga     2460

gacauuaucc cauugaauaa acucuuuacc acagaguacg agaucgaaca cauuaucccu     2520

cagucacggu auuucgauga caguuuuucu aacaagguua uuugcgagag ccuggugaau     2580

agacuaaaag auaacuacau aggucugggc uucaucaagc aguuucaugg guauaaaauu     2640

uccgacaaga cuaagguauu cgaagucgag gaauacgagg auuucgugaa aaagaauuau     2700

gcaaacaaca ggucaaaacg aagcaagcuc cugcuugagg aaauccccga ggaaaugauu     2760

gagcgccagu ugaaugacac ccgguacauc ucuaaauaca uauccggaau ccugaguaac     2820

auuguuagaa agaaggagaa ugacgaaggc gugaacagca aagauaucau uccagguaau     2880

gggaagauca caucucaauu aaaacaggac uggggccuca acgaugucug gaaugaccug     2940

auacuuccca gguuugagcg uaugaacgaa cugaccaagu ccucauauuu cacugugugg     3000

aaugagaaau accagaaguu uuugccuacg guaccagugg aguucaguaa gggauuuagc     3060

aaaaagcgca uugaucaccg gcaucacgcc cucgacgcuc ugguuaucgc cugugcaaca     3120

aaagaucaug ucaaccuacu gaauaacaag ucggccaaau ccgaaaugaa gagauaugac     3180

cucaaaagga agcugaugaa guucgagaaa aagaccuacc agcacucuca aacuggcgaa     3240

cgaaucguga aagaugugcc ggucgaguuu cuuaagcccu ggaaugacuu caccguugaa     3300

gcuaaagaga acuuggaaaa gauuauagug agcuuuaagc agaaucugcg ggugaucaac     3360

aaagccacaa accacuauga gaaguggauu gagaaagaua auguaaaggu caaaggguua     3420

guggaacagc agggacucaa cugggcaauc cgcaagccuc ugcauaagga caccguuuca     3480

gguaaagugg accuuccagg cauuaagguc cccaaaggga agauccugac ugcgacaaga     3540

aaaaguauag auacguccuu cgaccuaaag accaucaauu cuauuacuga uacaggagug     3600

caaaagaucc ucaaaaacua ccuggaguau aagggcaaua gcuccgaaga ggcuuuuuca     3660

agcgaaggua uugaggacau gaacaauaac aucgagaaau ucaaugaagg gaaguugcac     3720

cagccuauaa ccaaaguuag gauuuucgag cugggcagua aguuuccauu aggacagauc     3780

ggcaacaaaa agcauaagua cguggaaaca gccaaaggga cuaaucucuu cuuugcaauc     3840

uacgaggaug acaagggaaa acggaccuau gagacgauuc cccugaacga aguaaucgag     3900

cgacaaaagc agggccuugc cccggucacc cugaaaucua agaaugauuu cuacuugugc     3960

ccuaacgacc ucguguaugu ggcugccgaa ggugaguccg ucaauaagau agauuuuaaa     4020

uacgcaagcc aggaauuuau ucacaacauc uauaagguug ugucguucuc agggucucag     4080

auuuucuuug uaaaacaaaa uaucgcugug aguauaguca acaaggccga guucuccaca     4140

cugaacaaaa uggagagagc gguggacgga aagaugauua aggauuguug caucaaacua     4200

aagaucgacc gcauuggcaa uaucacuaaa agcuga                               4236


<210>  3311
<211>  4239
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3311
augaugacca agaacauccu gggccucgac cuugggacaa auagcauugg augggcccug       60

auaaaacaga acgcugagga aagaaagggu uccaucuugg gcauggggac uaggguguua      120

cccaugucuc aagaugcacu gggagacuuc ggcaaaggua auucagucag ucagacggcc      180

gcgcggaccg gguaccgcgg aguucgacgu cuaagagaga gguuucuccu gcggcgcgaa      240

agacuucaca gggugcugaa cauuuugggc uuccucccug auuauuacgc ugcccagauc      300

gacuuugaua agcgguucgg caaauuuaag gcagagacag aaccaaagcu gccguauaau      360

aacgaggggu ucauuuuuaa aaauagcuuc gaggaaaugc uguccgacuu uaagaucucu      420

cagcccgagc ucuuaucgga aaacaaaaag auaccuuacg auuggacuau cuauuaccug      480

cgcaaaaagg ccguaucaca acgaauugag aaggaggaac uuguguggau cuugcugaau      540

uucaaccaga aaagaggaua uuaccagcua aggggugagg acuuugauga agagaaggac      600

aaaaccuucg ucacacucaa uguggauaag guuauugaca gcggcgaaaa agugaagggg      660

aucguccugu uugaugugua cuucaccaac ggauggaaau augagaagca gauaacuaag      720

ccagaggacu ggcuuggccg gaguaaagag uucauuauca cagagucccu guugaagaau      780

ggugaaguaa aacguaccuu uaaggcuguu gacucugaga aagauuggau ugcaaucaag      840

gccaagacgg aacaagagau cgagauugcu cauaaaacug uggggagcua cauauaugac      900

gccauccucc agaaccccaa gcagaaaauu aagggaaaac ugauccgcac cauagaaaga      960

aaguucuaca aggaggaauu aaggaaaauu cuggagaagc agaaagaauu ucacccugag     1020

cuccaaaaug aggaucuuua uuccgacugc guccgggaac uguaccgaaa caacgaugcg     1080

caucaguugu cacuagcaaa gaaagacuuc auccaccugu uuguggagga uaucauuuuc     1140

uaucagcgcc cacucaagag ucaaaagagc ucugugucca auuguacacu ggaaugcaga     1200

aaauacaagg acaaaaacgg cgaggagagg auccaguuuc uuaaggucau ucccaaauca     1260

aauccuuacu aucaggaguu ccggauaugg caguggauga gcaaccugaa gaucuaccgc     1320

agagaugacg auguuaauau uaccagugaa uauuugaaaa cuacagaaga cuaccaagac     1380

cucuuugugu uccugaacac caggaaggag guagaucaga aggccuguuu aaaacauuuu     1440

aagcugaaug agaaaacgca ccguuggaac uauguggaag acaagcggua cccaugcaau     1500

gagacuggca caaugauccu agaacgauuc aaaaaggucg gggauucugc ugacaacauc     1560

uuuaccagag agauggaaca gcagaugugg cacauuauau auuccgugaa ugauaagacu     1620

gaguacgaaa aagcccucaa gagcuucgcu aaaaagaacg accuggauaa uguuucguuc     1680

uuugaggcuu ucaggaaauu uccgcccuuc gacucugagu acggauccuu uucagaaaag     1740

gccaucaaga aacuuuugcc ucugauucgc acagguaagu auuggaacug ggaggcggug     1800

gaugagaaaa ccaagaaacg gauccaaaag augauuacgg gcgaauggga cgaagauauc     1860

aaggacaaua uaagagagaa aacccucagu cugcagaagg agagcgauuu ccaggggcuu     1920

ccacuguggu uggcccagua cgcagucuau ggaaggcauu ccgaagcauc uguggccggc     1980

aaauggaaca gcguaaacga cauggaggaa uaccucaagg aguuuaaaca acacucacug     2040

cgcaauccca ucguugaaca ggugauuaca gaaacuaucc gagucgugaa ggacauuugg     2100

aucaaauaug guaacggggc ugccgauuuc uuuaaugaga uacauauuga auuaggccgg     2160

gagaugaagc ugccugcaaa ggaacguagu aaaaugaccc agaagaucuc cgagaacgag     2220

aauacaaacc uaagaauuaa agcccuccug gucgaaauga ugaaugacag cgaggugcac     2280

aacguuaggc cauacucucc caugcagcaa gaaauccuua agauauauga ggauggagcu     2340

cuuaauucag aaaucagugu ggacgaugac auucugaaaa ucagcaagac ugcgcagccg     2400

uccccuucug aucucaagaa auacaagcug ugguuagagc agaaauaccg cucgcccuau     2460

accggcgaca uuaucccacu gaacaaguug uucacaaccg aguacgaaau agagcacauu     2520

aucccucaaa gccgguauuu ugaugacucc uucucaaaua aaguaaucug ugaaaguucu     2580

gucaacagau ugaaggauaa uuacauuggg cucggauuua ucaagcaguu ccaugguuau     2640

aaaauuagcg acaagacuaa aguguucgag guugaagagu acgaggauuu ugugaagaaa     2700

aacuaugcau ccaauugguc aaagaggagc aagcugcuac uggaagagau acccgaagag     2760

augaucgagc gacagcucaa cgacacgcgc uacauuucua aauacaucuc cggcauucug     2820

aguaacaucg uccggaagaa agaaaaugac gaugggguga acagcaagga cauaauccca     2880

ggaaauggca aaauuacauc ucagcuuaag caagauuggg guuugaacga cguauggaau     2940

gaucuggugu uacccagauu cgagaggaug aacgaacuca ccaaguccaa ugacuuuacu     3000

guuuggaacg agaaauauca gaaguuccug ccuaccgucc caguggaauu uucaaaaggg     3060

uucaguaaga aacguaucga ucaccgccau cacgcccuug acgcucuggu gauugccugc     3120

gcaacacggg aucaugucaa uuugcucaac aaucagagcg ccagaucgga gaugaagagg     3180

uacgaccuga aacgaaagcu aaugaaguuu gagaaaaagg cuuaucagca cucccaaacc     3240

ggcgaacgga ucguuaaaga ugugccggug gaguuccuga agcccuggga caacuuuaua     3300

guagaagcca aagagaaucu cgaccgcauu aucgucucuu ucaagcagaa ccugagagug     3360

aucaauaagg caacuaacca cuacgaaaaa uggauugaga agaacaaugu uaaagugaag     3420

gcgcuugucg agcagcaggg auugaacugg gcuauaagga aaccucugca uaaggauaca     3480

gugagcggca agguugacuu accagggacg aaagugccca agggaaaaau ccucaccgcc     3540

acucggaagu caauugauac aaguuuugac augaaaacca ucaauuccau uacagauacu     3600

gguauccaaa agauacugaa gaacuaucuu gaauacaaag gcucuuauga ggacgcauuc     3660

agcccugaag ggaucgagga uaugaauaag aacauugaaa aauucaauga cggaaagcag     3720

caccagccaa ucauuaaagu acgaaucuuu gagcugggcu ccaaguuccc ccuaggucaa     3780

auagggaaca aaaagcauaa guacgucgag accgccaaag gcacgaaucu cuuuuucgcu     3840

auuuacgaag augacaaggg aaaaagaacc uaugagacaa ucccgcugaa cgaagugauc     3900

gagcgccaga agcagggcuu ggccccugug acuuuuaaau caaagaauga uuucuaccug     3960

agcccaaacg acuuagucua uguugcagcu gagggggaaa guguggauaa ucucgacuuu     4020

cagucugacu cccaagagua cauuaacaau guauauaagg uggucagcuu cucgggauca     4080

cagaucuuuu ucgugaaaca gaacguugcc gugucuauag ucaauaaggc ggaauuuagu     4140

acccugaaca aaauggagag ggccauugau ggccguauga ucaaggaaug uugcgugaaa     4200

cuuaagauug accggaucgg uaacaugaca aaguccuga                            4239


<210>  3312
<211>  4245
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3312
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cuucguggag       60

gaagcugaga augaauccga gaagucuuca auaaucaaac ugggugucag aguuaacccc      120

uugacagugg augaacagca aaauuuugag aagggcaaaa guauuacuac gaacgcagac      180

aggaccuuaa agcggagcau gcgccgaaau cugcagcguu acaagcagag aagggagaac      240

cuaaucgaua uucucaugaa aaauggguuc acauccgacg auacuauccu gucugaaaac      300

ggaaaucgga ccacauuuga gaccuaucgc cuuagaucga aggccguagc ggaaaagaua      360

ucacugcagg aguucgcuag gguguugcuc augaucaaca agaaacgggg cuacaagagc      420

agucgcaagg ccaaagguac ugaggacggg gaacugauug auggaaugga ggucgcaaag      480

aaacuguaua auuacaaccu cacaccuggc caauuaugcc ugcagcuuuu gaaugaaggc      540

aagaaacauc ugccagacuu uuaucgaucc gaucuacagg ccgaguucga cagaaucuuu      600

gauauucaga ugaccuucua cacggaauau cucacucugg agcuuaagga ggaacugagg      660

gggaaaaagc gggacgcugu gugggcaauc ugugccaaga uauuuaaaga gaagggauuc      720

gaauugauug guaccaaacg ucaaggcucu acagcugagc agaagcacga aaacuacagc      780

uggcgcgcca aagggcucuc cgagaagcug gaguuagaac agcugguugu gguccuccag      840

caaaucaaua uucagcuuaa cucaaguagc ggauaccugg gcgcgaucuc ugauagaucc      900

aaggaguugu uuuucaacaa acagacugug ggucaauauc agauggcagu acuagacaag      960

aauccgaacg ccgggcugag gaauaugguu uucuaccggc aggacuaucu cgaugaauuu     1020

aacgcuaucu gggagaaaca ggcccuguuc caccccauuc uuaccgacac acugaaggau     1080

gaaauacgag acgugaucau cuuuuaccaa cgcagauuga aaucacagaa gggacucauu     1140

agcuucugcg agcuggagag uaggaagaua gaagucgaga uugcaggcca gaauaaaacc     1200

aagauggugg gcucucgggu gaucccucgc uccagcccau uauuucagga guucaaaauc     1260

uggcaaucgc ugaacaauau ugaagucuuu gggaagggua aaaagucuaa gaaacagaag     1320

gagcagacgc uauucgauca gacugaagac ccccucgagu uuggaggcag aagggaacug     1380

uaugaggaag agaaagagcu uuuggcucug gaacucucca ucaagaaaga gcugaagaaa     1440

gccgauauuc uuaagcuguu guucgacaac ccuaaggaac ucgaucugaa uuuuaacgcg     1500

guucaaggga auuuaacaca ggccacccug uucaacgugu acaaaaagau aaucgagcua     1560

ucaggacaug aaauugacgu agagaaagau agugcaagca agauccucaa auccaucgag     1620

gaaauuuuua aggcucugaa uuauaacacu gagauacuuc acuucgacuc ugaaaagagc     1680

caggaccagc aagccaugua cauguugugg caucugcucu acucauuuga gggcgauaau     1740

aguaacacag guaacgaaaa ucugaucaac aaaauuucca aguuauaugg guucgagaaa     1800

gaguacgcua agauccuggc caaugugagc uuccaggacg auuauggcuc ucuuucagca     1860

aaagccauua guaagaucuu gccauaccuc aaggaaggac acaaaagcga cauagcuugu     1920

gaucuggcgg gcuauaagca cuccgcaucu ucgcuaaccc gugaggaacu ggagaacaaa     1980

acguacaagg accggcucga gauccugccc aaaaauaccc uucgaaaccc ugucguggaa     2040

aagauuuuga aucagaugau caacguuauu aaucugauca uagauagcag agaguuaaag     2100

aaauccgcca aggaaaggga ggaacucaca aaaucaauua gugagacuac caagcugcau     2160

caacaguaua aagacauccu ugcuaaggcc ccauuuaacc ugacaaauac uucucgcaac     2220

gauauuaucc gguacaaauu guacgaggaa cucaaggaca auggguauaa gacccuguac     2280

agcaacaaau auauaucccc ggcagcccua uucucaggag auuuugacau cgagcacauu     2340

aucccccaga gcagacuguu cgaugacucu uuuuccaaua agacacucga acugaggagu     2400

auuaacaucg agaaagguaa caagaccgcu uacgacuuca uaaaaggcaa guauggggaa     2460

acuggagagg auaauugccu ugacaaguac uugaaccgca uugagacgcu guauaaaaau     2520

ggcaucuuaa agaaaagcaa guacaacaaa cucaagaugc aggauaagga caucccugau     2580

gguuuuauug aacgagacau ccgggauaca caauacauug ccaaauaugc aaagacccug     2640

cuuggggacc uggugaaauu cgucgugucu acuuccggcu caguaaccga gcgucuaaga     2700

gaagauuggc agcucguuga cgugaugaag gagcugaauu gggaaaaaua caugcaguug     2760

ggacugacag aggaguuuac caacgaagag ggccauagga uacgcaagau caaggauugg     2820

acuaaacgga augaccacag acaccaugcg auggaugcuu uaacagucgc cuucacgaag     2880

aggcaguuua uucaauaucu caacaaucug aacgcacgac gcaucaguga cgaacagggg     2940

auuagcaauu cggagaaaga cgagcggaac aaugugucca ucaccacuaa cgauaugaaa     3000

cuuaauacaa gagacguccu gggaauagag aaaaaggaau uguacaggga uaaccguggu     3060

aagcuccgcu ucaaagcccc aaugccccug cuagaauuuc gggaggaagc uaagagacac     3120

cuggagaaca cccuuaucuc uauuaaagcc aagaauaaag ugacaacuau caacauuaau     3180

acccucaaga aagcaggcgg gguuuauaag aagacgcagc ugaccccuag gggacaguug     3240

cauaacgaga caaucuacgg cagccaaaaa caguauauag ugaaggaaga gacuauuaau     3300

gcuaaauucg acguagaaaa gaucgccacc gucacaaacc caguguaccg agaggcgcug     3360

uuaaaacggc ucgaacagaa uaacaaugau cccaagaagg ccuucacugg uucaaacgcu     3420

cugaccaaaa agccgaugug guuugacgag caacagacgg agaaaguucc ugaaaaggug     3480

aaaacaguca guuucgagac caucuacacu auucgcaagg aaauaucccc cgaucuuaag     3540

aucgagaaag ugguagacga aaaggugaga cagauucuga auaacagguu gaaugaguau     3600

aacgggaaug caaaacaggc cuuuucuaac cucgaugaga auccaaucug gcugaacaag     3660

gaaaaaggca uuacaaucaa gcggguuacc auaacuggaa ucaacaaugc ucaagcccua     3720

cacgagaagc uggacaaaga uggcaagccu auucuugacg gggaaggcaa aaagcagccc     3780

gucgacuucg ugaacaccgg aaauaaccau cacguggcag ucuacuguga ugcggacggu     3840

aaucuccagg agaacguuau cagcuuuuau gaggccguga cacgaagaaa ucugggguug     3900

ccaauuaucg auaaagacua caagaaagau gacggauggc aguucuuauu uuccaugaag     3960

caaaacgaau auuucguguu ucccaaugag ucaacgggcu ucaacccuaa ugaaguagau     4020

cugcucaacc cagacaauua cagccugaua aguccgaacc uuuuucgcgu ccagaaguuc     4080

accauuaaag auuauuucuu uaggcaccau cuggagacua auguggaauc uaacuccaca     4140

uugaagggua ucaccuggcu acguugcggc cugaacaaua ucgacaacau uaucaaaguu     4200

cggauaaauc acauugggca gaucauucau gugggagagu acuga                     4245


<210>  3313
<211>  4248
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3313
auggccaagu ucaacaaacc cuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguua uaaccgagga uuacagagug ccugcauuua gguucaaggu acucgguaau      120

acagaaaaag agaagaucaa aaagaaccuu auuggcucaa cuacguuugu gccagcccag      180

agugcgcaag ucacccgggu guuccgcguu aaucgacgua gaaucgacag gcggaaccac      240

cgcauugcuu aucugagaga uaucuuucag aaggaaauag agaaagugga caagaauuuc      300

uacaggcggu uggaugagag cuuucgcguc uuaggggaca aauccgagga ucugcagauc      360

aagcagccgu ucuuuggaga caaagagcua gaaacagccu aucauaagaa guaccccacu      420

auuuaucacc uccgaaaaca ucuggcagau gccgacaaga acucuccugu ggcugauauc      480

agagagguau acauggcacu uucgcacauu cugaaauaca ggggccauuu cuugacccuc      540

gacaagauca auccaaacaa uauaaacaug caaaauucau ggauuaacuu uaucgaaagc      600

ugccaggagg uuuucgaucu ggaggugagu gaagagucca aaacaauugc cgcuaucuuu      660

aagucuagcg aaaaucggca ggagaaaguc aagcagaucc uguccuauuu ccaacaggaa      720

cucuuaaaga aagacaaguc aauuuucaaa cagcugcuuc aguugcuguu uggucuaaag      780

accaaauuca aggacuguuu ugagcucgag gaagaacccg aucugaacuu caguaaggaa      840

aauuacgacg agaaccuuga aaaccuguug gggacucucg aggaggauuu uagcgacgug      900

uucgccaaac ugaagauauu acgugauaca auccugcucu cugacaugcu uaccuauacg      960

ggagcgacuc acgcacgcuu uuccgccacc augguggaaa gauacgagga acacaggaaa     1020

gaucugcaac gguucaaguc auuugucaaa cagaauuuga gcgagcagga cuaucuagau     1080

auuuucggcc gaaagacacc uaacggcuuu gacguugaua aggaaacuaa aggguacgug     1140

ggauauauca guaauaagau gguacugacc aacaaacaaa agacaauaca gcagaauuuc     1200

uacgacuaca uuucugguaa aaucaccggc aucgaggggg cugaguauuu ucucaacaag     1260

auuuccgacg gaacguuccu gcgcaagcuc agaacuacag auaauggcgc caucccaaac     1320

cagauucaug cauacgaacu ggagaaaaua aucgaaaggc aaggucggga cuaucccuuc     1380

uugcucgaga auaaggauaa acuguuaagc auucugaccu uuaagauccc uuacuaugug     1440

gggccacuag cuaaaggaaa caauucgcgc uucgccugga ucaagagagc gacuucucag     1500

gacauucucg augacaacga ugaggacaca aggaauggca aaauacgucc guggaacuac     1560

cacaagcuga ucaauaugga uaagacccgg gacgccuuua uuacgaaccu uaucggcaac     1620

gauauuaucu ugcugaauga aaaaguccuc cccaagcgau cccugauaua ugaggaagug     1680

augcuucaga acgagcugac cagaguuaaa uacaaggaca aauacgggaa gacacauuuc     1740

uuugauucag aauugaggca ggagaucauu aauaaccucu ucaagacuaa uaguaaacgc     1800

gugagcuccg caaugcuguu aaacuaucug gagaauuaca ccaaccuaca agcugucgaa     1860

aucgugucug gaauugagaa ggguaaaagc cucaauucaa cacugaagac uuauaacgac     1920

cuuaaaacca ucuuuaguga agaguugcug gacuccgaaa uauaccagaa ggagcucgag     1980

gaaauuguaa agguuaucac aguguucgau gacaaaaaga gcauuaaaaa uuaucugacc     2040

aaguacuuug gccgguuaga guuccugaac gaagagagaa ucaaucagcu uucuaaauug     2100

agguauacug gguggggacg cuacucagcc aagcuccugc uagauauacg agacgaagau     2160

acgggcuuua accugaugca guuccuccgg aaugacgaug agaaccguaa ccugagucaa     2220

cuuaucagcg acuccacauu gucuuuugau ucggcuauua agagcaucca guccaaauca     2280

accgucgagg acgauguguu cgacgaaauu aagaaacugg ccgguagucc ugcaaucaag     2340

agagggauau uaaauucuau uaaaaucguc gaugagcucg ugcagaucau uggcuaccca     2400

ccccagaaca ucguuauuga aauggccagg gagaauauga cuaccgagga aggacaaaag     2460

aaagcuaaga cacgcaagac caaacuggag agcgcgcuua agaacauaga aaauuccgug     2520

cuggagaacg gcaaaguacc ucacucagac gaacaguugc agagcgagaa gcucuaucug     2580

uacuaucuac aaaaugggaa agacauguac ucucuggaua agacuggauc cccaacaccc     2640

cucuaucggg acagacugga ucaguacgag gucgaccaca ucauuccgua uaguuuccuu     2700

ccuaucgaua gcauugacaa caagguguug acgcauaggg aaaauaacca gcgaaaacug     2760

aauaacaucc ccgauaagga gaccguugca aauaugaaac cauuuuggga aaaguuauac     2820

aacgccaaac ucauaucuca gacuaaguac caacgccuga caaccuccga gcggacaccu     2880

gacggugugc uuacugaauc aaugaaggcu ggcuucaucg agagacagcu ggucgagacc     2940

aggcagauua ucaaacacgu ggcccguauu cuagauaauc gcuuucucga cacgaagauc     3000

auaacccuga aaagucaguu gauuacaaac uuccggaaca cuuuucauau cgcaaagauc     3060

agagaacuga augauuauca ccaugcccac gacgcuuacu uagccguagu gauugggcaa     3120

acccuccuga aagcauaucc caaacuugcg ccagagcuga uauacggaca ccaugcucac     3180

uucaacaggc augaagagaa uaaggccaca auccgaaagc acuuguauag caacauuaug     3240

cgguuuuuca auaaccccaa uucgaagguu uccaaagaca ucuggaacug caaucgcgau     3300

cucccuauua ucaaggacau aaucuacaac ucucagguca auuuugugaa aagaacuaug     3360

auuaagaagg gcgcauucua uaaacagaac aucauuccaa augauagcuc agugaccguc     3420

gacaagaggu uuccgaucaa aaacaagaaa uaccuggauc ccaagcuaua cgguggguau     3480

uacgaacgga auagugcccu guccguuuuc auaauuggcc agucuuucaa caaaaauaac     3540

aaacuuaagg uggugaagga guuuuaugac aucaacauca uugauuaccc ucucuucaau     3600

acggacccaa ugagcuuuuu caacgaugac uccgagaaug gauuucugac aaagaacaau     3660

uugcaaucag uacaggcuuu cuauaaaauc cccaaguaca gccugauaca gaaaaacgau     3720

ggcacccgaa uguuauuuga aaguacuaca aauuuccaca aggccaccca auuuaagcuc     3780

acugaggcac agaacgaacu guucuuucau augaaaagac uucugaccaa gacagacuuc     3840

auggauuuga aaucugagga guccauuaag gaaagccagg acuuuaucau gaaucacaaa     3900

aacgaguucg acaauauugu caacgggcuu uucggauaug cugaacgcca gcuggguucg     3960

aagucacuau uuacgcaagc ccaugaggcg uacaagcuga ggaaauaccc uguguucucu     4020

cuuaaugaag agaccauccg uuauuacuau gauaacuuuc uccggcuguu caguuugacu     4080

aaguccggca caagcucugg guacguugug ggauuauuug aggccccaaa acugagaaau     4140

gucguguaua gggcuaccaa ggacgaaagc aacgcaacac ucauacacca guccauuacu     4200

ggccuguacg agacccgcau cgaucuuuca aaacugggug aagacuga                  4248


<210>  3314
<211>  4248
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3314
augaaccuga gaaauguggc caaggagggc aucaacuaca auauugggcu cgaccuugga       60

accgguagcg ucggcugggc uguuacagau gaacaggggg agcuguucuc cuuuaaagga      120

aagacuacgu ggggcucuag gauauucccc accgcagaaa cagccgcgga cacucgguug      180

aaacgcgguc aacgacguag auaugcuagg cggcgcugga gauuagaucu gcuacaguca      240

cucuuugagg aagagaucaa gaaaguggac ccugaguucu uuaccaggcu gcggcagagu      300

caccuucuga aggaagauaa gacauacgcc gcaauuuuga gcuccaaagg ggccacccgc      360

ccacucuucc cggagggaga agcugaggua gaguauuaca agaaauaucc cacuaucuac      420

caucugcgaa aggcacugau ggaaucuaac aaucaggagg acauuagacu caucuauuua      480

gccuuucaca acauagugaa agcuaggggc aauuuccugc aucaagauac accuggccuu      540

accgccucga acgcgaauau gggggacgca gucgaaaagu ugugcucagc ccuggaggau      600

ugguguagca gucagaacau cgaaugcgcu auucggaaga aucagaaauc cauccaggac      660

cuaauggagg gaaagacgau ugagccaucu gaaagcaacg cccucaaaga gacuuuugau      720

gcaucccguu caagucgcaa ggacaaagcu agcgugcugg ccgguuggau cgcgauacaa      780

cccggcgaac aguucgagcc uaagcaggcc aaagaugcau cuaaggaaau ugcuaaugcc      840

guuuuugggc uuuccgcuaa caugaagaaa guguucgucg agcuggagga agaccagaag      900

aucuacuuga auaacgacga gcaaguggaa gccuuuuaca ccguaugucc agaugaggga      960

guugcacucu ucgaagcccu gcagucaaug uauagcgcuu acguguuaau gggcauucug     1020

aaaggugcgg acgggggaag uaucucuucc ugcaaggucg cagccuauga gcaguacaaa     1080

gaggaucuca aacuucugaa gacauuggug agagaauuug cucccaagaa guaugacgag     1140

uuuuucaggg gcgauaucuu ugacgccaaa aagggcuucc ggacucgaaa cuacgauccu     1200

gcaaccgcca aaggguauac aaaguacaau agcgugcgcg gagcuucgua cgacgccuuu     1260

aagaaagaag ucgagaagcu auucgagggu accgcagcgg aagaggaugu uagauauacg     1320

gacaugaugc aaagguuuga ugaaggcacu uuccugcggc gccucaaaac aucugacaac     1380

ggguccauuc cauaccagcu gcaccuugag gaaauggugg cuauaaucga gaaucagaag     1440

accuuuuauc cguuccugga ggaaaacaaa gagaaguuug aaucauuggu aacuuuccgu     1500

auucccuacu augugggacc ucucacacag aagaaugcca ggccagacaa ccauggcaau     1560

gcacguuuug ccuggaguca acggaaagag ggucaggaaa acgcucgagu cuaccccugg     1620

aauugggagg aggugaucga uagaaagcag agcgccgcaa aauucaugga aaggcugacc     1680

gggacgugua ccuauauauu agaggaaccu guucugccac gcuacucccu acucuacgag     1740

gaauauugcc ugcuuaacga guugaauggc gcucggugga gagacauggg aggcgauugg     1800

cacagguucg acuacgagac acgcauggau auuaucgaag accuguuuaa gaaaaagaaa     1860

gggacuguga aguaugccac cgucgcggau uggcucgugc gagagaagca ccggucuugu     1920

gccgaaguaa aaggagguca gggcgagaca ggguucgaga gcaagcuguc aaguuacguu     1980

uucuucaaag gagugcuuca ugucgacgaa aucgaugagg cugacauucc caugaucgag     2040

gaacugauuu gguggaacac uuuguuugaa gaucguucca uacucaagga gagcaucgag     2100

gacaaauaug gcgauagacu gaaugacgaa caaauuaagg caaucugcaa gaaaagguuc     2160

accgguuggg ggcgcuuauc uaagaaacug cuauguggca ucaaggccca gacagacaac     2220

ggaccgugcu caauuaugga ugugcuccgg gagggcaauc cuaacaacga cgcuagaagu     2280

gccaccaugg ucuuuaugga aauaaucagg gaugacaaau ucggguuuac uaagcugauu     2340

gaggaagaga auaagaaacg aaucagcucc guggaugacu ucgauguuaa cgaccuuccc     2400

ggaucuccag cagugcgccg gacgguaaau caggcgaaga gaauugucga ugagaucgug     2460

gguauagccg gcugugcucc ucagaacauc uuuauugaag uuacaaggga cgaggauggg     2520

cguaauaaag gacgccggac caagagaagg uacgacgcca uccaagaagc agugaaaguc     2580

uugaagaagg agcagaaaga auauuucgau gcuaacgugg ccgaccugcu caagaauacu     2640

gcacccgccg cucuggacga ugagcgauua gcgcuguacu uuaugcagaa cggcaaaugc     2700

auguacugcg guaaggaccu ugauauuaau caguuggaca aauaucaagu agaucacauc     2760

cucccacagu cguacgugaa ggacgauagc uucgagaaca aagcccuggu ucauuccgac     2820

gauaaucagc ggaagucaaa caguaugcua cuggacgauu cuauaauucg caagaugaaa     2880

cccuauuggg aagcacucag aagcgcuggg cugaucggcg agaagaaauu caagaaucuu     2940

accagggucc ggguguccga caaagaauug ggaggcuuua uugcccgaca aaugguggag     3000

acaucacaga gcaugaagcu gaccucuuua cuccuggagc aggccuaccc ugaaacucag     3060

gucagaccag uuaaggcugg guucuccagu gaccuucgca ggcgucugaa auuggugaag     3120

ugucgggaga ucaacgauua ucaccacgca caugacgccu accucgcgug cgaaguggga     3180

caauuuauag cucugagaca caauaaacua uaugagaacc cgaucggcua cagccaugcc     3240

guaaaggauu ucauugcaaa aagggccaag uacgcuucug aacacgguaa gcccauggac     3300

acauauggga ugccuggauc cucaaguuuu gucaucagcu cguucaugac guccggcuuu     3360

gaugaggaga ccggugaaau uuucaaagac gcaugggaug ccucucuggc ggcugugcgc     3420

auccaucagg cccucaaccg aaaggacugu uacauaagca gaaugccaga ggaaacuuca     3480

ggcgcauuuu gggaugccac aauuuauagu cccaaacaga ccaagaaaaa gcugucccuu     3540

ccuuugaaga aagggcugga cccacagaag uacggaucuu auagccggga gcaauucgcu     3600

uacuuuuucg uuuaugaagc caaaaagauc aaaaagaagg aggagacaaa agaauacgug     3660

uucgcauccg uccccgugca gguugcugcc uuaguggcgg ccgauccgaa ugcacucauc     3720

gaguuugcua aggccgacgc ugaagagagg ggccugaaau ucgcaaagau uguacgcgaa     3780

aaagucuaca aguaucagcu uaucgaggug aacgaugaga gauuuuucgu gacugggcug     3840

agaaccgucc ggaauggacu acagauugcc uuuucagaac aagacaggaa cauaguuagc     3900

ggcaucgugg aggguaauag uucuaacaau aaccuccugg acauuuacga uaaaaucacg     3960

aaucgcuaug ccuuguacgc uucccgauuc gggaagcugu uaaaccucag cggauauaga     4020

gaaaauuuua uucaggcguc gcuggaggac caggagcggg uagugcuugc acugaucuca     4080

uuguucaacg ccaggaccaa uaugauagau cucucugcua ucggcaguuc caagacaguc     4140

gguaacauga gcaugacuuu ugccaaagaa auggcaaugg ccgacaaucg cuucgcuuuu     4200

auugaucaau cugugaccgg caugcuggag agaaagacau accuauga                  4248


<210>  3315
<211>  4251
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3315
augggcagca gaaucauucc caugucccag gacauacugg gggaguucgg aaaggguaac       60

ucugugucac aaaccaguga aaggacagcc uaccggagca cucgccgacu ccgugagaga      120

uuucuucuga ggcgggaacg cuugcacaga gucuuaaaua uccuggauuu ccuaccuacg      180

cauuaugcuu cccagauuga cuuuaaaaag aggcucggca aauucaagga gaacaaagaa      240

ccaaagaucg cauacgauaa uaagucuuuu cuguuccuug acucguuuaa cgagaugcug      300

aaagauuucg agcaggaaca gccgaauaug uugauuaacg agaaggggga aaagaagcuc      360

auccccuaug acuggacccu guacuaucug cggaaaaagg cccuccgcga gaagauauca      420

aaagaggaau uaagcuggau cauucugaau uuuaaccaaa agcgaggaua cuaucagcuu      480

agaggcgagg augaagagga aaauccuagu aaauugguug aguuccacuc ccugacagug      540

guagacgugg ucccagauga gcccaucaag gguaaacuag aaacuuggua cucuauuauc      600

cucgacaacg ggugggugua caggcggagc uccaagaccg agcuguauga uuggaaagac      660

aagauaaagg aauuuauugu uacaaccgau cuuaauuacg acggaacugu gaaaacagac      720

aaggagggca acgucaaacg uucauuccgc gcgccuagug aaaccgauug gacgcugauc      780

aagaaaaaga cugaguauga gauugaacag agcucuaaga ccaucggcga guacaucuau      840

aauacauugc ucaaauaccc agaacagaag auuagaggga aacugauaag gacuaucgag      900

cggaaguauu acaaagaaga guuaaagcaa auucugcuca agcagaccga guuucauaac      960

gaacuuaaag acgauucccu guacuaugac ugcuugcgag agcuauaccg caauaacgaa     1020

ucacaccagc uguuccucga gaagaaaaac uucgcucagc uguuuaucga ggauauaauu     1080

uucuaucaaa gaccccuuag gagccagaag agucugaucu cuaauugucc uuuggaauuu     1140

uacaaauuca aggacuccgu gggaaacgag aagguacagc aucucaaagc caucccaaag     1200

agcaauccgu uuuaucaaga guuccggauu uggcagugga ucucgaaccu gucuauuuac     1260

uccaaauuag augacucaaa uuuuacaagu cacuucuuug auagcaccga cgauugggag     1320

ucccuguucg aaaugcuaaa cggucgcaag gagauagagc agaaacagcu ccugaaguuu     1380

cuuuuggaga aaaagggcuu caaggggaaa ucucugagcu cagaaaucga aaaguauaga     1440

uggaauuacg uugaggacaa aaaguacccc ugcaacgaaa cgaggagucu cauucaaucc     1500

cgucuggaca aaguggcaaa ucuuccugag agcuuucuga cucggcacgu cgagcaggaa     1560

uuguggcauc ucaucuauuc ugugacagau aagaucgagu ucgaaaaggc ccuggacacc     1620

uucaguaaaa aguacaaaau ugauacucca acauuugugg acgcuuucaa gaaauguccc     1680

ccuuuuaagu cagaguaugg cagcuucucc gaaaaggcaa ucaaaaaguu acugccacua     1740

augcgaaccg gaaaauacug ggaucucgac aacauagaua aggccacgaa acagagaauu     1800

gagaagauca uuucugguga guaugacaag gauaucaggg accgcguccg ggaaaaagcu     1860

auaaaucugc uuucggagac cgauuuuaac agauugcagc uguggcucgc ccaauacauc     1920

guuuaugaca ggcacagcga agcgacaucc cuggauaagu gggagacuau ugacgacauc     1980

gugaccuacu uacauucauu caaacagcac agucugcgca aucccauugu agaacaggug     2040

aucacagaaa cuauacgagu cgugaaggau auuuggucua acuacgggaa uggaagcaaa     2100

gacuauuuua acgagaucca cguugaacuu ggccgggaga ugaaguugcc ggcagaggaa     2160

cguaaaagga uucaggagaa gaacucccaa aaugaagaua ccaaccucag aaucaaggcc     2220

cugcuaucag agaugcagaa ugacccuaac guggaaaaug uccgccccua cagcccagug     2280

cagcaggaga uacugaaaau cuaugaagag ggcguacuca acucuaauau ugagaucgcu     2340

gaugacauuc ugaagaucuc caaaacagcc caaccuagua gcucugaucu uaagcgguac     2400

aaauuguggc uggaacagaa guaucagucc cccuacaccg gggagauaau uccauuaaac     2460

aaacucuuca cucccgaaua ugagaucgaa cauaucauuc cucaaucaag auacuuugac     2520

gauaguuaca auaacaaagu uaucugcgag agcgcaguga auaagcugaa agacaacucg     2580

cuuggauaug aguucauuaa ggguuacaau ggccagauag ucgaaugugg guuuggcaaa     2640

aaggugaaga ucuucuccau ugaugaguau gaagacuuug uccagaaaaa cuacgcuaau     2700

aacaggucua aggccagccg acugaugaug gcggauaucc cagagaaaau gauugagcgc     2760

caguugaacg acacgcggua uaucucaaag uacauaagua auauccucuc caacauugug     2820

agaucugaaa gcaaugacga uggaguuaac uccaaaaauc ugaucucagu gaacgguaag     2880

auuacaaccg uacuaaagaa agacuggggg augaaugaua ucuggaacga ccugauacuc     2940

ccgagguuca uucguaugaa ucaaaucacu gauagcgaca guuucaccac auggaacgag     3000

cgcuuucaga aguucauccc caccgucccu auugaacugu cuaaaaauuu uuccgccaag     3060

cggauagauc acagacauca cgcaauggac gcucuuguga ucgccugcgc uacuagggau     3120

cauguuaacu ugcugaauaa cuuauaugcc aaaagcucgg cacgauacga ccucaaucgg     3180

aagcugcgca uuuucgagaa gacaacguac aaagauauca agaccggcca gauuaucgaa     3240

agagagauac caaaagccuu ucuuaagccc uggcagaacu ucacuacaga cgcuaaagac     3300

gcgaucgagu caauuaucgu gucuuauaag caaagucuga gggucauuaa caaauccacc     3360

aauuacuauc agaagugggu ggaaaaggau ggaaucguga aaaaggagaa aauaaagcag     3420

gaaggcaaaa acugggcaau ucggaagccu cuacacaagg acacaguaag cgggcucguu     3480

cagcugucuu gggucaaagu gccaaaggga aaagugauga cugccacccg aaagagcuug     3540

gauuccucau uugaccugaa aacgaucgcu accaucacag auacugguau ucaaaagauc     3600

uuaauaaagu accucgagag uaaaggcaau aaccccgaaa gcgccuucuc uccggagggg     3660

auugaggacu uuaauaacaa uauccaccug uauaacgaug gaaagaaaca uagaccuauu     3720

accaaggucc gccuuuacga aaucggcucc aaauucccac ugggugagaa ggggaauaag     3780

aacgcaaaau auguugaagc cgcuaagggc acaaauuugu uuuucgccgu guacgcaaac     3840

aauaaaggag agaggucauu cgaaacuaua ccccucaacc uggugauuga ccgucuaaag     3900

cugggccuua gugcgguacc ugagaccaau gagaaagggg auaaccuccu guuucacuug     3960

agccccaaug accuggucua cgugccaucc gaagaugaga uggaaaacuu aaagcucauc     4020

aaccuggagc auaucucuaa agaccagcuu aaucggauuu auaagauggu uucgagcacg     4080

ucaaacagau guuucuuuau caaguccgag auugcucuga guauagugaa uaaaaucgaa     4140

uacucuccuu ugaacaagau ggagaaaagc auugaaggaa ucaugaucaa ggaugucugc     4200

cucaaacuac aggugaauag gauugguaac cuggagcgcu auuucuccug a              4251


<210>  3316
<211>  4257
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3316
augaagguga aaaacauccu gggccucgac auugggguca ccagcauagg auucgcccac       60

guuacagaga augaaaagga uaacaaaucc cauaucaagc agcuuggugu gagaauuaau      120

ccccugacug uagacgagca aacgaacuuu gaaaaaggca ggccugcuuc ugugaaugca      180

gaucggaccu ugaagcgcgg ggcccgaaag auguuaucac guuacaaaga cagaaggaag      240

aaccugaucg agauucuaag uaaaacagga uucaucaaua gcgaauccau acucgcggag      300

gauggcaaga acacuaccca cucuacaugg gcucugcggg ccaaagcauc gcgcgagaga      360

aucgaaaagg acgagcuugg uaggguccug uuggccauua auaagaaacg gggguauaag      420

ucaagccgca aagcuaagaa cgaagaugag ggacaggcaa ucgacggcau ggccauugcu      480

aaacgacucu acgaggaaaa ucugacccca ggccagcugg uguaucagcu cuuaagagag      540

gggaagaaau acaucccgga uuuuuauagg agugaccugc aagaugaacu ugacaagguu      600

uggaacuucc agaagcaguu ucaucaggag auauugacug auacauucua caaagacauu      660

caaggaaagg gucagcgggc caccuccgcg ucuuuuuaug gcguguacgg guucaauacg      720

gcagaaaaca aaggaagccg ugaggagaag cgccugcagg ccuacgcuug gagauccgac      780

gcccuaacua aacagcucac cagggaagag gucgcauuug ugaucacaga aauuaauaac      840

aaccugaaua acucaagugg cuaucuuggu gcuaucagcg aucggucuaa ggagcuguuc      900

uuuaauaaga aaacuguagg gcaauacuug uaugaacagc uccagcaaaa cccccacacc      960

cgacugaaga aucagguuuu cuaccgccag gacuauuuag augaguuuga gacaaucugg     1020

accauucagu ccagauacua uccugaacug acggacgauc ucaaaaggga gauacgggac     1080

guggucaucu ucuaccaacg caaacuuaaa ucacagaagg gacuggugag cuucugcgaa     1140

uuggagagua aggaaauuau gaucgagaaa aacggcaagg ccgcggugaa aaagauaggc     1200

ucuaaagucg ccccaaaguc cagcccccua uuucaggagu ucaagauuug gcagguucug     1260

cauaaugugg auaucaaaaa caagaaagac aagacuaaaa gaccucucga ugacucggaa     1320

aaggaguacc uguuuaauga acuuaacauc aaagggaauc ugaaggcagc uaagguaauu     1380

caauugcuca cacugaacaa aaaugagacc gaguuaaacu auacucagcu ggaaggaaau     1440

aggacacaga aggcccuaua cgaggcuuau cucaaaaucc uggauaccga agguuacgac     1500

gugaacaacc uuuugggcau uaagucuaau aaagacgagg ucuuccugga ugaccucgau     1560

gugaacgccc uggaaauaaa gacgaugauc aagcagauuu uuaaugcacu uaacaucaau     1620

accgggaucc uggacuucga ugccgaguug gacggaaaag cauuugagca acaggcgucc     1680

uaucaccucu ggcaccuguu auacucauau gaagaggaug gcaguaagag cgguaacgaa     1740

caucuguaca aacuacucga gaauaaguuc ggguuuaaac cagaacacgc acguauucug     1800

gccggcauaa cacuuuccga cgauuacgga aacuugucug cuaaggccau gcggaagauc     1860

uauccccaga uuaaagagaa uaacuucagc acugcaugug agcugguugg cuaccgacau     1920

ucaaagaaua gucucaccaa agaagagaac caggaaagac cucugaagaa uaaauuagag     1980

cugcuuaaga agaacuccuu gaggcaacca guggucgaaa agauccucaa ucagaugauu     2040

aaccugguga acgagcuaau cgacaagaau agcgagaaag augaacaggg gucuauaauc     2100

gaguauuuuc gcuucgacga aauucggauc gagcuggcca gagagcucaa gaaaaacaca     2160

gaugaaaggc gcgaccugga cgcugccauu cgagagggaa aucuucggaa cgaaaagauc     2220

uacaaauugc ugcaagcaca guuugguaua aagaauccga cucguaacga uauuaucaga     2280

uauagguuau acgaggaacu caagaauaac ggcuauaaag accuguacac caauucauac     2340

auuccccagg agaaacuuuu cgggaaagag aucgauauag accacaucau uccucagagu     2400

aagcuguucg augacagcuu uuccaacaaa acauugaccu auaauacuac gaacaaggau     2460

aagggaaauc aaacaggcuu cgacuacauc gaaucuaaau augguauuga ugggaucgag     2520

gcguacaccg cucgcauaga aaaccucuuu gagucgggca agaaaaauaa ggaagaggga     2580

guaagcaaag ccaaguauca gaagcugcua aagaaggagu ccgaaauugg cgacggguuc     2640

aucgagcggg aucugagaga aucacaguac aucgcuaaaa aggccaaaga gcuccugcuu     2700

cagauuacua ggaguguugu ggcuaccucu ggaagcauca cagcccgauu gcgcgaagac     2760

ugggaucugg ucaacgugau gaaggaguua aaccucccaa aguaucgggc acugggucuu     2820

accgagaugg aagagagaaa auacggccaa aaggucgaag ugauuaaaga cuggacuaag     2880

agggacgauc accgucauca cgcuauggac gcccugacaa uagcguuuac gaaacauaau     2940

cacauccagu accuaaacca ucucaaugcc cgcaaggaug agcagcacaa ggagcacaaa     3000

auuaucaacg cuauugaaaa uaaggagacc auccugauaa cugacaaauc cgggaaccgg     3060

aagagaguuu ucaaagcacc caugccuaau uuuaggcaga cagccaagca auauuuggaa     3120

gaggugcuga ucucacauaa agcuaagaac aagguaguca ccaaaaauaa gaacaaaauu     3180

uuaggaaagg agaaaaucca ggaaacacuc acuccacgag gccagcugca cgaucaaacc     3240

auuuacgguu ucaauuggug gagccggauc acgcuuucua agaagguguc ccgcaacgag     3300

auagagaaaa uuaucgacaa uaacaccaag uuuuuccugc aggaauuuau ccaguugaaa     3360

gggaguauug ccaaggcauu cagcgaugcg gaacucaaua aacugaugua uaagggcaag     3420

auacuaaaag agaucgaagu ugccauuccc ugcuacacac agagaaucga cauuucuaag     3480

guguuuacug auucccaaaa aucaaacagu gcuaaggaga aaaauaucaa gaacauacug     3540

gaccuuaagg ucaggaacau ccuccagaau cggcugagcg aguucaaagg agauuucaag     3600

aaagccuuuu cggacuugga caagaacccg auuugguuca auaaagaaaa gggcaucucc     3660

auuaaacgag ugaucauauc ugggguaaag aacguggagc cucugcauua uaagaaagau     3720

cacuuaggaa augacauucu cgauggcaag gaaaacagaa uccccguuga cuuuaucagc     3780

accgguaaua accaccaugu cgcaauuuac caggaugaga aagggaaucu gcaggaaaag     3840

guggugucac uuuucgacgc ugucaaacug guuaacgccg augagccagu gaucgacaag     3900

acauauaauc aaggauuggg cuggcaguuu cucuucacua ugaagcagaa cgagauguuu     3960

guguucccua augauaacac cgguuuugac cccaaagaaa uagaucugcu agacccaagu     4020

aauaagaaac ugauuucccc caaccuuuuc cgcguacagu cuaucagcau uguccaauac     4080

ggcaacaaua ucgugaggga uuuuaaguuc cguaaccacc ucgaguccgu ucugaaugac     4140

gacaaagugu ugcaugggau aacguuccag cagauuaagu cauuaggacc ucugcucaac     4200

aucgucaagg ugcggaucaa ucaccugggc gauauuguua gcguggggga auacuga        4257


<210>  3317
<211>  4260
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3317
auggccaagc cccagaacua caccaucggc cuggacauug ggacaaauag cgugggaugg       60

acugucacgg augaccaaaa caauauacuc agaaucaaau ccaagaaagc uauuggugca      120

aggcuuuucg aggaaggcaa gguugccgcg gagcggcgcu cuuuucgaac cacacguaga      180

aggcugucac ggcgcaaaug gagaaucaag uuguuagagg agcuguucga ugaagagauu      240

aguaaggugg acccuaacuu uuucgcuagg agccacgagu ccuggaucuc uccaaaagau      300

aagcgggaac gcuauucggc cauaguauuu ccgacucccg aggaagacaa aaaguuccau      360

aaugaguacc cuaccaucua ucaccuacga gauagacuca ugaaagacga ucagaagcau      420

gacauuaggg agaucuacgu ggcaauucac cauaucauga aguaucgggg gaacuuucug      480

cacgaugaau caauagagac auaccguagc ggaaugaguu cccuuggcgg uggggacgaa      540

cgcaccauuc ugaauacuaa aaucuuggau gaccucaacg acgccuucgc ugauaaucug      600

gcaacacagg gaauuucugu caacagcgcc uccgcugacc agaucaauga gaucgugacc      660

ucaggccacc ugaacguuga uagucaaaag gccauugcga aacucuuacu gccaacgacu      720

cuuagcucug acgauaagaa acaggacaag aaaauaaaga aguuggcaac cgaugccgcu      780

aaaaaugugg ccuccgcaug gcugggcuau aaggcuaacu uugccgcgau ccuaaauauu      840

caggaagucg acaaagauaa gaaaaagauc uacucauucg cccuccaggg ggcagacgag      900

gaggauaagg uggaaacacu ggagagcgua cuuagucaag accagauaga aauuguugac      960

agaaucauca ggauucggca ugcuaucgug cugggagaga uuauacccgu cgguaugacu     1020

uugucugaag ccaugaucga gaaauacgau cagcacgcua aggaccucaa aaaccugaag     1080

accgugauuc gaaacuuaaa agaucgcaag aaugccguga agcugcaggc aaucuaugac     1140

cucuacguca acaauagaca ugccgauaca gcuaaagcga ugaaacuugc aggcguuaag     1200

aagaggaccg agcuguugga accugaggaa uuuaacaaau ccaucgagaa gcuacuggac     1260

gauagcccag ugucggccga aauuaagaaa cggauagcug agaaggacuu ccucccccug     1320

caacgcucua auuccaacgg gguaaucccu aaucagcuuc accagauuga gcuggaugaa     1380

aucauuaaaa agcaaucaaa auauuaccca uuuuugggag agacgaacga acacgccagu     1440

gcagacgugg ccagaaaggc uccgacuaaa cucgaucagc uguuaacauu cagggucccc     1500

uauuacgugg gcccucugau caccaaggag gagcaggagg aacaggacgg ucauagcuuc     1560

gccuggauga uacguaagaa uccaaaagau cacgaaaaga ucacucccug gaacuuugac     1620

gagaaaguug accggauggc auccgcgaca caauucauua agcgaaugac cacgaaagau     1680

accuaucuac ucggggaaga cgugcugccu ucuagcucac uuaaguacca guuguuuaca     1740

guccugaaug agcucaacaa ucugagaauc aaccauaaga aacuuacuag ggaggaaaag     1800

gaacagguga uugaagaucu guucaaaaag uacaaaaccg uaaaggcuga caaguuuaaa     1860

aauuauuggc agaagggaca cgccggcuug gauguuaaaa uccaaggcau ggcagaccca     1920

gagaaguuca acaguacaau guccacuuac auagaucucc agaaaauuuu uggggaccag     1980

cuggaugacg cccagcgcca aaacgauuuc gaaaagauca uugaguauag caccaucuuu     2040

gaggaccgga agauauuagg agaaaaacug agagcugauu ucguguggcu aucugaggac     2100

cagaucaaga ggcucugccg ccugcgacuu caggguuggg gccgguuguc acugaaacuc     2160

cugacacguu uaaccgacgc cgaugggcag aauguccugg aaagacuuuu gaacaguaau     2220

gacaacuuua ugcaaauugu gaaggauccc gucaucaaag caaagauuga gcagaaaaau     2280

cagaagcucc ugagggcuga cuggagcgaa aacgccacug aguccauccu agaugaggcg     2340

uacacgucuc cgcaaaauaa gaaagccaua cgccagguga ugcggguugu gcaugacauu     2400

guaaaggcuu gugauggaaa ccugccugca aaauucgcca ucgaauuugc uagaucgcag     2460

gacgaugacc ccaagaggac agucgcccga gcagaucgcc uccagaaaau cuaugcgcaa     2520

auuagcgacg aggugguuuc ccagggcgug aaggaccagc ugucagguau gaagagucuu     2580

aaagaucggu acuauuugua cuucaugcaa gggggcagag acgccuacac cggaaagcca     2640

cugaauaucg auagguuauc ugacuaugau auugaccaca uaaugccuca gagcuucauc     2700

aaagaugacu cccucgauaa ccguguccug gugacucgcg cuguaaauaa ccagaaguca     2760

gacaaugugc ccguucuucu guacggcaac cacuuggucg ccgaucucgg gauuaccauc     2820

cgggaaaugu gggacagaug gcagaaaagc ggaaugauuu cuaagaggaa gcuggcaaau     2880

cuacugacag auccagcuac ccucuccgag uaucgagccg aggguuuuau caaccggcag     2940

cugguggaaa caagucaagu gauaaaacuu acugcaacga ucuugcagag cgaguucccc     3000

gacucuaaga uuaucgaagu cccugccaaa uacaacucca uucugcgcaa gcaguuugac     3060

uuauauaaau caagagaggu uaaugauuac caucacgcua ucgacgcgua ucucaguacc     3120

auagugggca acuaccugua ccaaguguau ccaaaucuua ggcggcuguu cguauacggg     3180

gaauuuaaga aguucagcca uggagaggag gaaucguccc accaggccgu ccgaagagug     3240

aaaucuauga acuuucuaga ugaccuccug cgcggcaagc acggugauga cauuuauugc     3300

aggguuacug gggaggugau cuucagccgu aaagauauca uuucaaaguu gaaacaggca     3360

uacaguuuua agcagaugcu ggucacacaa gaaguguuca ccaagaaauc cgcuuuauuu     3420

gaccagacag uuuauccguc ucccgcccgg gauagcaaga cuagauccgg ccucauaccu     3480

aggaaacagg gaauggacac cgagaucuac ggcggguauu caggaaauaa ggaugccuac     3540

uucgugcugg cucagguagu caaaagcaag ggucauacgc uucaaguggu gggcguccca     3600

auucgcgcac ugcgagccuu ggcgaacugu aguaauuacu cugaacagcu ccugaaaauc     3660

guuaaggcuc aggugcuauu uaacaagaaa accgggaagg ccaaaaaggg aauuuccgac     3720

guaagaaucc ugcuugauaa aauacccuau cggcagccug ugcucgacgg cagcgcauuc     3780

uacaugcugg guucgucaac auauaaguac aauuugaagc aaaucucucu gagucagcag     3840

uccaugaaau auauuuuaga cuacaucgau gacccaaacu ucaauaagca cgagaugacu     3900

aaaauugauc agcaaaacga gaaggaaugc cuccugagcg ucuaugacga gauccuugau     3960

aaaauggaca aguaccuguc uuuguacgau auaaaugggu uuaggaagaa acuccaugug     4020

ggccgcgaca aguucauuaa acuggaaguu gagaagcagg ccgaagcuau caaaaacgug     4080

cuacguggau uucacgcaaa uagcgauaug accaagcugu ccgaccuugg ccucucaaca     4140

agucuggggu ucaugcaggc cagcgaugga aucaaguugu cugacaacgc ggucuuuauu     4200

uaucaauccc ccacuggcuu auucgagaga cgggugaaag uuucagaccu gcucaaguga     4260


<210>  3318
<211>  4260
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3318
augaugauca agaacauucu gggccucgac cuugggacca auagcauagg augggcccug       60

aucaaacaga acuucgagaa uaaguacggu gaaauuuugg gcaugggguc cagaaucauu      120

cccaugucuc aagauguguu aggagaguuu ggcaaaggua acucagucag ucagacagcu      180

gcaaggacug gguaucgggg aguucgccga cugcgugaaa gauuccuacu caggcgggag      240

cgccugcaca gagugcuuaa uguacugaac uuuuugccug aacauuacgc cagccagauc      300

gacuucgaga agagguuugg caaauucaag uaugagacgg aaccaaagcu cgcguacaau      360

aacgauggcu uuuucuuuaa aaauuccuuc gacgagaugc ugggggauuu uaaggaccac      420

caguuccauc ugcucgaaaa cggacggaaa gugccguaug auuggaccau auacuauuua      480

cgcaagaaag cucugucuca aaagaucgag aaggaggaac uugccuggau uuugcugaau      540

uuuaaccaga aacgagguua cuaccagcua agaggcgagg acuucgaaga ggaaaaggau      600

agggucuucg ugcggcucaa aguugaccgu guggucgauu cgggggagaa uaucaaggga      660

aaaauucugu augacgugua cuuugagaac ggcuggaagu augaaaaaca gaucguaaag      720

acagaggauu ggauaggucg cacuaaggaa uuuauuguua ccgagucaau ccuuaaaaau      780

ggggacacaa agagaaccuu uaaagcagug gacagcgaaa aggauuggau ugccaucaaa      840

acuaagacag agcaagagau cgacaagagu cacaaaaccg ucggagcuua cauuuaugaa      900

gcacuguugc agaaccccaa gcagaaaaua aagggcaaac ucgugaggac gaucgagcgg      960

aaguucuacc gagaggagcu gaagaaaauu uuagaacgcc agaaggaguu ucauccugag     1020

cugcaauccg augaccucua uaacgauugc aucagagaac uuuacaggaa uaacgaggcc     1080

caccagauaa cucugucuaa aaaggacuuc gugcacuugc uacugaauga ucucauuuuc     1140

uaccagcggc cacugcgcag ccaaaaaucc ucagucagua acuguacccu ugaguucaga     1200

aaguauaagg acgaaaaugg cguugagcau gcucaguacc ugaaaacaau ccccaagagc     1260

aacccuuauu accaggaauu uagggugugg caguggaugu ucaauuugaa caucuaucgu     1320

aaagaugacg auacuaaugu aaccaaggag uuucucucuu ccacagaaga cuacgagacc     1380

cuguucgaau uuuuaaacaa ucggaaagag guggaccaaa aggcccugcu acgacacuuc     1440

aaacucaacg agaagacgca uagauggaau uaugucgaag auaagaaaua cccaugcaac     1500

gagacuaaga caaugauuuc aaccaggcug gaaaaagugg agaauuaccc cagcgacuuc     1560

cuuacucgcg aaauggagca gaacaucugg cacauuauau auaguguuaa cgauaagauc     1620

gaguacgaaa aagcguugaa gucuuuugca cacaagaaua accuggacga aaauuccuuc     1680

uuugaagccu ucaaaaaguu uccuccauuc aaaagcgagu augggucguu uucugaaaag     1740

gcuauuaaaa agcuccugcc gcuucugcgg uugggaaagu acuggaacug ggaguccauc     1800

gauagaaaau cacaggguag gaucgagaag auuauaacag gcgaguucga cgggaauauc     1860

aaagauaagg ugcgcgaaaa agccauucau cucaccgcag aaaacgacuu ucagggacug     1920

caauuauggc uggcucagua uaucgucuac ggccgacaca gugaggccag caugauuggu     1980

aaguggaacu ccgcggauga ccuagaggag uuccucaagg gguuuaaaca gcauucucug     2040

cggaauccca ucguggaaca gguaauaacg gaaacccuuc guguugugaa ggauaucugg     2100

uugaaauaug gcaauggagc caaggacuuc uuuagcgaga uucacaucga acugggcaga     2160

gagaugaaac aaacaaagga ugagaggguc gacgcaacua aacgcauuac cgaaaacgag     2220

aauacaaacc uccggaucaa ggcucuguua gccgaaauga ugaaugacaa cucaguggag     2280

aauauaagac cuuacagucc aaugcagcag gaaauucuga agaucuacga ggaugggauu     2340

cuuaaauccg acaucgagau agaugacgau aucuugaaga uuagcaaaac ugcucagccc     2400

ucuucaagug accucaagag guauaaacug uggcuagaac aacgauacaa gagcccuuau     2460

accggacaga ucauuccacu gaacaaacuc uucacauccg aguacgaaau cgagcauaua     2520

auuccccagu cucgcuauuu cgaugacucg uuuagcaaca aaaucaucug ugaauccgcc     2580

gucaauaagc ugaaagauaa cuacauuggu cuuggcuuca ucaagcaauu ugggggaaug     2640

auuguggagu gcggcuucgg uaaaucaguu gagauauuua aggcagaaga guaugaagac     2700

uucgugaaga aacacuacgc caauaaccgg aguaagagaa auaaauugcu guuagaggag     2760

aucccggaaa agaugauuga uaggcagcuc aacgacaccc gucacaucuc uaaauacauu     2820

agcgggguac uguccaauau cguccgccag gaggauggca cugacgaagg agugaacuca     2880

aagaauauag uuccuggcaa cgggaagauc acgacacagc uuaaacaaga cuggggacug     2940

aaugaugugu ggaacgaguu gauucucccc cgguuugaaa gaaugaauca gcugaccaac     3000

agcacugacu ucaccucuug gaauaagaac caucagaaau uucuaccaac agucccuauc     3060

gaguucucca aggguuuuag uaaaaagagg auugaucacc gacaucacgc uauggacgcg     3120

cuggugaucg caugugccac caaggaucac auaaaccucc ugaauaacca gagcgcuaaa     3180

ucugacacua agcgguauga ucuuaaacgc aaguugauga aauucgagag aguaguguac     3240

ucccauucaa agacaggcga aaagauugag aaggaaguuc ccaagcaauu ccugaaacca     3300

ugggacaauu uuacggucga ugccaagaac aaaauggaga ccaucaucgu gaguuucaag     3360

cagaauuuaa gggugauuaa caaagcaacu aaucacuaug aaaaguggau agagaaggac     3420

gggaucaaac ggaaggagcu cauuaaacag gaaggaacaa acugggccau ccgaaagccc     3480

augcauaaag agaccgucag cggcaagguu gaucugccug guauuaagau cccaaaaggg     3540

aagauacuua cagcuacuag aaaaucgcug gacaccuccu uugaccuaaa gacgaucaau     3600

ucuauuaccg auacaggcau ccagaaaauu cucaagaacu accuggaguu caagggaagc     3660

ucagaauugg ccuuuagucc ggaaggcauc gaggacauga auaaaaacau agagaaguuc     3720

aaugauggga aaccccacca auccauuaac aaggugcgca ucuuugaacu gggaucuaaa     3780

uucagcgugg gccagacugg uaauaagaaa gacaaguaug uagaggcagc gaaggggacc     3840

aacuuauuuu ucgcugucua cgaagauaaa aauggaaaga gguccuauga gacaaucccu     3900

cucaacgagg ugauugaacg ucagaaacaa ggccuggacu caguuagcuu uaagaacaaa     3960

gaggauuucc uucugugccc aaaugacuug guguacgucc ccagugaaga ugagcucgaa     4020

aacaucuaca auauagacuu uaagaaccug acuaccgaga agacggguaa uauuuauaaa     4080

gugguuucuu ucuccggcaa ccagaucuuc uuuauucggc aggauaucgc cauaagcauu     4140

gugaauaagg cagaguucuc gacacuaaac aaaauggaaa gagccaucga cgggucaaug     4200

aucaaggaga auuguauuaa acugaagauc gauaggcuug gaaacauugc uaagauauga     4260


<210>  3319
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3319
auggccaagc ccaaagacua caccaucggc cuggauauug ggacaaacag cgugggaugg       60

gucguuacug acgaucagaa uaacauacuc agaaucaagg guaaaaaggc uauuggcgca      120

aggcuuuuca cggaggggaa aguggccgcg gaacggcgcu ccuuucgaac cacacguaga      180

aggcugucuc ggcgcagaug gaggaucaag auguuggagg aauuauucga cgaggaaauu      240

gcuaagguag auccuucauu uuucgcccgg cugcacgaga guuggaucag cccaaaagac      300

aagcgcaaac gauauuccgc aauaguguuu ccgucucccg aggaagauaa gaaauuccau      360

gagucguacc cuacuaucua ucaccuaaga gacaagcuca ugaaggauga ccaaaaacau      420

gauauuaggg aaaucuacau ugccguccac cagaugauca aggcucgggg aaauuuucug      480

caugacgagu caguggagac cuaucguagc ggcaugaguu cccuuggugg gcgcucugaa      540

agaacaauac ugagcguuca gaccuuggag gaacucaacg aucuguucgc agagaaugaa      600

ggaacugagg agguggaacu gaacgucgcc uccgcugagc agauuaauga cauccucaca      660

ggcggccacu uaaacgccga cucacaaaaa gaaauuagua aucugcuuuu gccaagcucu      720

uuucccuccu ucgaugacaa ggcgaaagag aaacagguga aaaagcugau caacaaugua      780

gcaaccaaca ucucaaaggc cuggcuaggg uacaaagcug auuuuagcac gauucucaau      840

cuggccaagg uugacaaaga ucagaagaaa auauucgcau uugcccuuca gggagaagac      900

gaggaggaua aggugcaaga acuggagagu uugcucgaac agucucagac ugacaucguc      960

gauaggcuga uugagauccg gcacgcuaua guguuauccg aaauugugcc ugucgguaug     1020

acccugagcg aggcgaugau cgacaaguau gaucagcaua aagaggaccu caucacacuu     1080

aaggccguua uucgaaacac uaaagacaag aaaaaggcag cuaagcugca agccaucuac     1140

gauuuguacg ugaaaaaucg ccacgcugac cuagccaagg caaugaaacu gaccggcauu     1200

aagaaaagau cggaacuccu ggauccagag gaacuuaaga aggggauauc uucccuguug     1260

gacgauucac ccgaggccgu agagaucaaa cagaggcucg aagagaagac auuccugccu     1320

uuacagcgga guaacaacaa uggagugauu ccaaaccaac ugcaucaggu cgaacuagac     1380

gagaucauca aaaagcagag caaauauuac ccguuccucg cugaaaagaa ucccgaugag     1440

uccgaggaag cgcagaaaaa ggcaccuacc aagcuggacg cccuuuugac guuucgcgug     1500

ccauauuacg uuggccccau gauuacuaaa gaggaacaag aggcucagaa cggucacucu     1560

uucgccugga uggugagaag ggauccucag gaccacgaag caauaacacc auggaauuuu     1620

gagaagaaag ucgauaagau ggccagcgcu acccaguuca ucaaacguau gacuacaaag     1680

gacaccuauc ugcucgggga ggaugugcug cccgccucaa gucuuaagua ccaacuguuu     1740

acgguauuga acgaacucaa uaaccugcgg guuaauggaa agaaguuaac cuccgacgag     1800

aaagaacagg ugauugaggg ccuguucaag aaacagaaga cagucaaggc agacaaauuu     1860

gugaaguauu ggcaggcgaa acauaucggc gcugauauua aggucaaagg gcuaagcgac     1920

ccgucuaagu ucaacucaac uaugaguacc uacaucgauu uuaagaaaau auucggagac     1980

caacucaaug augugaaccg acagaaugac uuugaaaaga ucauugagua cagcacaauc     2040

uucgaggaua gaaaaauucu ggaagacaaa cuuagggccg auuuugcaug guuguccgag     2100

gaacagauca agcagcugug ccgccuccgg cugcaagguu ggggcagauu aucugacaag     2160

cugcuuacua gguugaccga ugccgacggg cagaacguuc ucgagcgccu guggaacucg     2220

aaugauaacu ucgugcagau aguaagcgac ccuaauauua aagcuaagau cgagcaagaa     2280

aaccagaagc uacugcgaga cucccaguca gauaaugccg ucgagaguau ucucgacgau     2340

gcauauacau cuccccagaa caaaaaggcu auccggcaag ugaugcgugu ugugcacgac     2400

auagucaaag ccugugaugg aaaugugcca gcgaaguucg ccaucgaauu ugcuagaagc     2460

caggaggacg auccuaaaag gaccacugca cgcguaaacc agaugcagaa gauuuacgac     2520

caaaucuccg augaaguggu uucacagggc gucaaggagc agcugagcgg uaugaaaucu     2580

cuuaaggacc gguauuacuu guauuucaug caagggggca gagaugccua cacgggagcu     2640

aaacugaaua uugacagguu auccgacuau gauguggacc auaucaugcc ccagaguuuu     2700

gugaaggaug acagccucga uaaccgaguc cugguugccc gcgcagugaa uaaccagaaa     2760

ucugacaagg ugccagcgcu ucuguucggc aauaagguag ucgccgauuu ggggauaaca     2820

gugcgggaga ugugggacaa auggcagaag cucggaauga uuuccaaaag aaagcuguca     2880

aaccuacuga ccgaucccga cgcucucacu gaauacaggg cccaagguuu uauccgucgc     2940

cagcugguug agaccaguca ggugaucaaa cuuacagcaa ccauuuugca gagcgaguuc     3000

ccugauucga agaucauuga agucccagcu aaguacaauu ccauagugcg gaaacaauuu     3060

gaccuguaua agucuagaga aguuaacgau uuccaccaug ccaucgacgc auacuuaagc     3120

acuauugugg gcaauuaucu cuaccaggua uauccgaacc ugaggcgaau guuugucuac     3180

ggggaguuca aaaaguuuuc aaguaacgcc gaggaauccg cucacgacgu ggcgcggcgc     3240

gugaaaucua ugaauuuccu ugaugaccug cuaagaggaa cacacggcga uaacaucuau     3300

ugcaggagca cgggugagau ugucuuuaau cggaacgaca ucauauccaa gcucaagcag     3360

gccuacucau ucaaacagau gcugguuacc caagaagugu ucacucgaaa gagcgcauug     3420

uuugaucaga caguauaccc caguccugag agagacucua aaaagcgcuc cgggcugauc     3480

ccaaggaaaa agggcaugga ucccgaaauu uauggaggcu acgcucauaa ucaggacagc     3540

uauuucgugu uagccaaaau cgagaagguc aagggguaca cccuccagau ugugggaguu     3600

ccuauccguu cgcuggagau aauuaacaaa gccgaagauu uuucaacagc acuucuggac     3660

aucaucaagu cuaaagugau guauaaugag aagacugguc aaccaaaacg gggcauuaag     3720

aacgucgaaa uauuggugaa uaagcucccc uacagacagc cgauccugga gaaagauguu     3780

aguuauaugc uaggguccag cgcuuacaag uacaacauga aacagauuac ccugucuagg     3840

cagagcauga aguauauccu ugacuacauu gaugacccua auuucacgaa acacgagaug     3900

accaugaucg acgaacaaaa cgagcgcgaa ugucuccugu ccguguauga ugagauauug     3960

gaaaaggucg acaaguaccu gccauuauuu gauggaaaua aauuccgaga gaagcuccau     4020

ucagccagaa aagaguuugu aagucugagc guggacgaug aagcgucugc uaucgagaag     4080

auucuucggg gccugcacgc caacuccgaa augcccucau uggagaaucu caaaaucaag     4140

ccugcacugg guuucuuuca gaugaagaac gggauuacac uaaguaacga cgccguucug     4200

cuuuaucaga gccccacugg ccucuucgaa aggcgcguga aagucuccga ucuguugaag     4260

uga                                                                   4263


<210>  3320
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3320
auggccaagc ccaaagacua caccaucggc cuggauauug ggacaaacag cgugggaugg       60

gucguuacug acgaucagaa uaacauacuc agaaucaagg guaaaaaggc uauuggcgca      120

aggcuuuuca cggaggggaa aguggccgcg aagcggcgcu ccuuucgaac cacacguaga      180

aggcugucuc ggcgcagaug gaggaucaag uuguuagaag aacuguucga cgaagagauu      240

gcuaaaguag auccuucauu uuucgcccgg cuacacgaaa guuggaucag cccaaaggac      300

aaacgcaagc gauauuccgc aauaguguuu ccgucucccg aggaggauaa aaaguuccau      360

gaaucguacc cuacuaucua ucaccucaga gacaagcuga ugaaagauga ccaaaagcau      420

gauauuaggg agaucuacau ugccguccac cagaugauca aagcucgggg aaauuuucuu      480

caugacgaau caguggagac cuaucguagc ggcaugaguu cccugggugg gcgcucugag      540

agaacaauau ugagcguuca gacccucgag gagcugaacg aucuguucgc agaaaaugag      600

ggaacugaag agguggagcu caacgucgcc uccgcugaac agauuaauga caucuuaaca      660

ggcggccacc ugaacgccga cucacaaaag gagauuagua aucuuuugcu gccaagcucu      720

uuucccuccu ucgaugacaa agcgaaggaa aaacagguga agaagcuaau caacaaugua      780

gcaaccaaca ucucaaaagc cuggcucggg uacaaggcug auuuuagcac gauucugaau      840

cuugccaaag uugacaagga ucagaaaaag auauucgcau uugcucugca gggagaggac      900

gaagaggaua aggugcaaga guuggaaagu cuccuggagc agucucagac ugacaucguc      960

gauagguuaa uugaaauccg gcacgccaua gugcuguccg agauugugcc ugucgguaug     1020

acccucagcg aagcgaugau cgacaaauau gaucagcaua aggaggaccu uaucacacug     1080

aaagccguua uucgaaacac uaaggacaaa aagaaggcag cuaaauugca agccaucuac     1140

gaucuauacg ugaagaaucg ccacgcugac cuggccaaag caaugaagcu caccggcauu     1200

aaaaagagau cggagcugcu ugauccagag gagcugaaga aagggauauc uuccuugcuc     1260

gacgauucac ccgaagccgu agagaucaag cagaggauug aggaaaagac auuccugccu     1320

uuacagcgga guaacaacaa uggagugauc ccaaaccaac ugcaucaggu cgagcuagac     1380

gaaaucauua agaaacagag caaguauuac ccguuccucg cugagaaaaa ucccgaugaa     1440

uccgaggagg cgcagaagaa ggcaccuacc aaacuggacg cccuuuugac guuucgcgug     1500

ccauauuacg uuggccccau gauaacuaag gaggaacaag aagcucagaa cggucacucu     1560

uucgccugga uggugagaag ggauccucag gaccacgagg caaucacacc auggaauuuu     1620

gagaaaaagg ucgauaaaau ggccagcgcu acccaguuca uuaagcguau gacuacaaag     1680

gacaccuauc ugcucgggga agaugugcug cccgccucaa gucuuaaaua ccaacuguuu     1740

acgguauuga acgagcucaa uaaccugcgg guuaauggaa agaaauuaac cuccgacgaa     1800

aaggaacagg ugaucgaagg ccuguucaaa aagcagaaga cagucaaagc agacaaguuu     1860

gugaaauauu ggcaggcgaa gcauauuggc gcugauauca aagucaaggg gcuaagcgac     1920

ccgucuaagu ucaacucaac uaugaguacc uacauagauu uuaaaaagau cuucggagac     1980

caacucaaug augugaaccg acagaaugac uuugagaaaa uuaucgaaua cagcacaauu     2040

uucgaggaua gaaagauccu ggaggacaaa cuuaggguug auuuugccug guuguccgag     2100

gaacagauaa agcagcugug ccgccuccgg cugcaagguu ggggcagauu aucugacaaa     2160

cugcuuacua gguugaccga ugcagacggg cagaacgugc ucgaacgccu guggaacucg     2220

aaugauaacu ucguacagau ugucagcgac ccuaauauca aggccaagau ugagcaagag     2280

aaccagaaac uacugcgaga cucccaguca gauaaugcug uggaaaguau ccucgacgau     2340

gccuauacau cuccccagaa caagaaagca auacggcaag uuaugcgugu gguccacgac     2400

aucgugaagg cuugugaugg aaauguacca gccaaauucg cgauugaguu ugccagaagc     2460

caggaagacg auccuaagag gaccacugcu cgcgugaacc agaugcagaa gaucuacgac     2520

caaauuuccg augagguugu cucacagggc gugaaagaac agcugagcgg uaugaagucu     2580

cuuaaagacc gguauuacuu guauuucaug caagggggca gagaugcaua cacgggagcc     2640

aagcugaaua ucgacagguu auccgacuau gauguggacc auauaaugcc ccagaguuuu     2700

gucaaagaug acagccucga uaaccgaguu cugguggcuc gcgccgugaa uaaccagaag     2760

ucugacaagg uaccagcacu ucuguucggc aauaaagucg uggcggauuu ggggauuaca     2820

guucgggaga ugugggacaa guggcagaaa cucggaauga ucuccaagag aaaacuguca     2880

aaccuacuga ccgaucccga cgcccucacu gaguacaggg cucaagguuu uauccgucgc     2940

cagcuggugg aaaccaguca ggucauuaaa cuuacagcca ccaucuugca gagcgaguuc     3000

ccugauucga agauuauaga agugccagca aaauacaauu ccaucguucg gaagcaauuu     3060

gaccuguaua aaucuagaga ggugaacgau uuccaccaug cuauugacgc cuacuuaagc     3120

acuaucguag gcaauuaucu cuaccagguc uauccgaacc ugaggcgaau guuuguguac     3180

ggggaguuca agaaauuuuc aaguaacgca gaagaguccg cccacgacgu ggcucggcgc     3240

gucaagucua ugaauuuccu ugaugaccug cuaagaggaa cacacggcga uaacauuuau     3300

ugcaggagca cgggugaaau cguuuuuaau cggaacgaca uaaucuccaa gcucaaacag     3360

gcguacucau ucaagcagau gcuggugacc caagagguau ucacuaaaaa gagcgccuug     3420

uuuaaucaga caguguaccc caguccuaaa cgagaugggu cuaagagauc cggccugauu     3480

ccacgcaaaa ggggaaugga ccccgaaauc uauggcgggu acgcacauaa ccaggauagc     3540

cacuucgucu uagcuaagau ugagaaggug aaaggauaua cccuccagau cguuggugug     3600

ccuauacguu cgcuggaaau uaucaauaag gccgaggacu uuucaacagc ccuucuggau     3660

aucauuaaau cuaaggucau guacaacgag aaaacuggcc aaccaaagcg ggggauaaag     3720

aauguggaaa ucuugguuaa caaacucccc uauagacagc cgauucugga gaaggacgug     3780

aguuacaugc uaggauccag cgcuuauaaa uacaauauca agcagauuac ccugucuagg     3840

cagagcauga aauacauccu ugauuauaua gacgauccua acuucacgaa gcaugaaaug     3900

accaugaucg acgagcaaaa ugagcgcgaa ugucuccugu ccgucuacga cgagauuuug     3960

gaaaagguag auaaauaucu gccauuauuu gacggcaaca aguuccgaga gaaacuccac     4020

ucagcaagaa aggaauuugu gagucugagc guugaugacg aggccucugc gaucgagaaa     4080

auucuucggg gucugcacgc uaauuccgaa augcccucau uggagaaccu caagaucaag     4140

ccugcccugg gguucuuuca gaugaaaaac ggcauaacac uaaguaauga ugcagugcug     4200

cuuuaccaga gccccacugg acucuucgaa aggcgcguca agguguccga ccuguugaaa     4260

uga                                                                   4263


<210>  3321
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3321
auggccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguug ugaccgauga cuacagagua cccgcauuua aguucaaagu gcucgguaac      120

acagagaagg agaaaauaaa gaagaaucuu aucggcucaa cuacguuugu cccugcccag      180

agugcgcaag ggaccagggu guuccggguu aaccgccgac guauugauag aaggaaucac      240

cggaucgcuu aucugcgcga cauuuuucag aaagagaucg aaaaggugga uaaaaacuuc      300

uacagaaggu uggacgagag cuuucggguc uuaggagaua aguccgagga acugcagaua      360

aaacagccau ucuuuggcga caaggagcua gaaacagccu aucauaagaa auacccgacu      420

aucuaucacc uccgcaagca ucuggcagau gccgacaaaa auucucccgu ggcugauauu      480

cgagagguau acauggcaau cucgcacauu cuuaaguaca gaggucauuu ccugaccuug      540

gacaggauca acccuaauaa cauaaauaug caaaacucau ggauugauuu uaucgaaagc      600

ugccaggagg uuuucgaccu cgagauuagu gacgaaucca aaacaaucgu ggccaucuuu      660

aagucuagcg auaaucggca ggagaaaguc aagaagauuc uguccuauuu ccagcaagaa      720

cugcucaaaa aggacaaauc aauauucaag caguuacugc agcuuuuguu ugggcugaaa      780

accaaguuca aggauuguuu ugagcuagaa gaggagccag accucaacuu caguaaagaa      840

aauuacgaug agaaccugga aaacuuucuu ggaagccugg aggaagacuu cucugaugug      900

uuugcuaagu ugaaagugcu ccgugacacu auccuguuau ccggcaugcu gacauauacc      960

ggcacgacuc gcgccagauu cucagcgacc auggucgaga gguacgagga acaccggaag     1020

gaucuccagc gauuuaagag cuucguuaag caaaaucuua gugagcagga cuaucuggau     1080

auuuuugggc gcaagacacc caacggauuc gacguggaca aagaaacuaa ggguuacgua     1140

ggcuauaucu cuaauaaaau gguguugacc aacccucaga agcagaaaac aauacaacag     1200

aauuuuuacg auuacauuuc cgggaagauc accggaaucg agggcgcaga auauuuccua     1260

aacaagauua gcgacgguac guuccugaga aaacucagga cuacagauaa uggggccguc     1320

ccaaaccaga uccacgcuua cgagcuggag aagauuauag accggcaagg agaagauuau     1380

cccuuucuuc uggagaauaa agacaaguug cucucgaucc ugaccuucaa aauuccuuac     1440

uaugugggcc cauuaucuaa gggcaacaau ucccgcuuug gguggaucaa aagaacuauc     1500

ucacaggaua uucuggacga uaacgacgaa gauacaagga auggaaagau acguccgugg     1560

aacuaccaga agcuaaucaa uauggacgag acccgggaug ccuucauuac gaaccucauc     1620

gguaacgaca uuauccugcu uaaugagaaa guuuugccca agcgaagucu gauauaugaa     1680

gaggugaugc uccagaacga acugaccaga gucaaauaca aggacaaaua cggcaagaca     1740

cacuuuuucg auagcgagcu uaggcaagaa aucauuaaua accuguuuaa gacuaauaac     1800

aaacgcgugu ccucugcaau guugcucgac uaucuggaga auuacaccaa cuuacaggcu     1860

guagagaucg uuagcgggau ugaaaaggga aaaucacuga auaguacacu aaagacuuau     1920

aacgaucuca aaaccaucuu cuccgaggaa cugcuugaca gcgagauaua ccagaaggaa     1980

uuggaggaga uugugaaggu caucacagug uuugaugaca aaaagucuau uaaaaauuau     2040

cugaucaagu acuucggcca ucucgaauuu cugaacgagg aacggauaaa ucaguuauca     2100

aaacugagau auaccgguug ggggagguac agugccaaac uuuugcucga uauccgcgac     2160

gaggauacug gcuucaaccu gaugcaauuu cuacgaaaug acgaugaaaa ccggaaccug     2220

agccagcuca uuuccgacuc uacgcugucg uucgauagcg cgaucaaguc cauucaguca     2280

aaaaguacag ucgaggacga cguguucgau gagaucaaga aacuugccgg aucuccugca     2340

auaaagcgug gcauuuugaa uagcaucaaa aucguugacg aacuggugca gauuaucggg     2400

uacccacccc aaaacauugu aauagagaug gcuagagaaa auaugaccac ugaggaggga     2460

cagaagaaag ccaagaccag gaagacaaaa uuagaauccg cucucaagaa caucgaaaau     2520

ucaguccugg aaaacgguaa agugccucau agcgaugagc agcuucaauc ugaaaagcug     2580

uauuuguacu aucuccagaa uggcaaagac auguacuccc uggauaagac cgggagucca     2640

acuccccuau aucgcgacca gcuggaucag uacgagguug accacauuau cccguauagc     2700

uuucucccua uugauucuau cgacaacaag gugcugacac accgggagaa uaaccaaaga     2760

aaacuuaaua acauacccga uaaggaaacg gucgccaaua ugaaaccauu cugggagaag     2820

uuguacaacg caaaacugau cucccagacc aaguaccaga gguuaacuac aucagaacga     2880

accccugacg gagugcucac agagaguaug aaggccggcu uuauugaacg ccagcuggua     2940

gagacucggc aaaucauuaa acauguggcu agaauccuug acaauagguu ccuggauacc     3000

aagauaauug cgcuaaaaag ccagcucauc acgaacuuuc guaacaccuu ccacaucgca     3060

aagauucgcg agcugaauga cuaucaucac gcccaugaug cuuacuuggc cguugucgug     3120

ggucagacac uguuaaaagc auaucccaag cucgccccag aacugauaua cgggcaccac     3180

gcucauuuua accggcacga ggaaaauaag gccacuauca gaaaacaucu uuauucgaac     3240

auuaugaggu ucuuuaauaa ccccgacucc aaggugucua aagauaucug ggacugcaau     3300

cgagaucugc cuauuaucaa ggaggucaua uacaacagcc agguuaauuu cgugaaacgg     3360

accaugauca agaagggcgc auuuuauaac caaaauccag ugggaaaauu caacaagcag     3420

auugcggcua auaaccgcua cccguugaaa acaaagguau caagucucga cacuuccauc     3480

uacggcgggu auggacccau gaacucugcc cugagcauua ucauaauugc agaaagauuc     3540

aaugagaaaa aggguaaaau cgagaccguc aaggaauuuc acgauaucuu cauuaucgac     3600

uacaagaaau uuaauaacaa cccuuuccag uuucuaaaug auacguccga gaacggcuuc     3660

cugaagaaaa auaacauaaa uagggugcuu gccuuuuaug ggguuccaaa guacucacuc     3720

augcagaaaa acgacggaac acggaugcug uucgaaagca agaguaauuu gcacaaggcu     3780

acccaauuua aacugacuaa gacacagaac gaguuauucu uucauaugcg aagacuccug     3840

accaaaucua aucuuaugga ucugaagucc gagagcgcca uuaaagaauc gcagaacuuc     3900

aucuugaagc acaaggagga auuugacaau auuucaaacc aacucucugc auucagucag     3960

aaaaugcugg gcgacacuac cucccuacgc aaucugauca aggguuauaa cgagaggaaa     4020

auaaaggaaa uugauagcuc ugaggaggug aucaaauacu ucuaugacaa uuuuaucaag     4080

auguucagcu uugucaaguc cggggcuccc aaaggcauua acgauuucuu uaacaauaag     4140

uacacagugg cccguaugcg gccuaaacca gacaagaaau uccuuaacgc gacgcucauc     4200

caucagucaa uuaccggacu guacgaaacu agaauagauu ugaguaaguu aggcgaggac     4260

uga                                                                   4263


<210>  3322
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3322
auggccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguug ugaccgauga cuacagagua cccgcauuua aguucaaagu gcucgguaac      120

acagagaagg agaaaauaaa gaagaaucuu aucggcucaa cuacguuugu cccugcccag      180

agugcgcaag ggaccagggu guuccggguu aaccgccgac guauugauag aaggaaucac      240

cggaucgcuu aucugcgcga cauuuuucag aaagagaucg aaaaggugga uaaaaacuuc      300

uacagaaggu uggacgagag cuuucggguc uuaggagaua aguccgagga acugcagaua      360

aaacagccau ucuuuggcga caaggagcua gaaacagccu aucauaagaa auacccgacu      420

aucuaucacc uccgcaagca ucuggcagau gccgacaaaa auucucccgu ggcugauauu      480

cgagagguau acauggcaau cucgcacauu cuuaaguaca gaggucauuu ccugaccuug      540

gacaggauca acccuaauaa cauaaauaug caaaacucau ggauugauuu uaucgaaagc      600

ugccaggagg uuuucgaccu cgagauuagu gacgaaucca aaacaaucgu ggccaucuuu      660

aagucuagcg auaaucggca ggagaaaguc aagaagauuc uguccuauuu ccagcaagaa      720

cugcucaaaa aggacaaauc aauauucaag caguuacugc agcuuuuguu ugggcugaaa      780

accaaguuca aggauuguuu ugagcuagaa gaggagccag accucaacuu caguaaagaa      840

aauuacgaug agaaccugga aaacuuucuu ggaagccugg aggaagacuu cucugaugug      900

uuugcuaagu ugaaagugcu ccgugacacu auccuguuau ccggcaugcu gacauauacc      960

ggcacgacuc gcgccagauu cucagcgacc auggucgaga gguacgagga acaccggaag     1020

gaucuccagc gauuuaagag cuucguuaaa caaaaucuua gugagcagga cuaucuggau     1080

auuuuugggc gcaagacacc caacggauuc gacguggaca aagaaacuaa ggguuacgua     1140

ggcuauaucu cuaauaaaau gguguugacc aacccucaga agcagaaaac aauacaacag     1200

aauuuuuacg auuacauuuc cgggaagauc accggaaucg agggcgcaga auauuuccua     1260

aacaagauua gcgacgguac guuccugaga aaacucagga cuacagauaa uggggccguc     1320

ccaaaccaga uccacgcuua cgagcuggag aagauuauag accggcaagg agaagauuau     1380

cccuuucuuc uggagaauaa agacaaguug cucucgaucc ugaccuucaa aauuccuuac     1440

uaugugggcc cauuaucuaa gggcaacaau ucccgcuuug gguggaucaa aagaacuauc     1500

ucacaggaua uucuggacga uaacgacgaa gauacaagga auggaaagau acguccgugg     1560

aacuaccaga agcuaaucaa uauggacgag acccgggaug ccuucauuac gaaccucauc     1620

gguaacgaca uuauccugcu uaaugagaaa guuuugccca agcgaagucu gauauaugaa     1680

gaggugaugc uccagaacga acugaccaga gucaaauaca aggacaaaua cggcaagaca     1740

cacuuuuucg auagcgagcu uaggcaagaa aucauuaaua accuguuuaa gacuaauaac     1800

aaacgcgugu ccucugcaau guugcucgac uaucuggaga auuacaccaa cuuacaggcu     1860

guagagaucg uuagcgggau ugaaaaggga aaaucacuga auaguacacu aaagacuuau     1920

aacgaucuca aaaccaucuu cuccgaggaa cugcuugaca gcgagauaua ccagaaggaa     1980

uuggaggaga uugugaaggu caucacagug uuugaugaca aaaagucuau uaaaaauuau     2040

cugaucaagu acuucggcca ucucgaauuu cugaacgagg aacggauaaa ucaguuauca     2100

aaacugagau auaccgguug ggggagguac agugccaagu uguugcucga uauccgcgac     2160

gaggauacug gcuucaaccu gaugcaauuu cuacgaaaug acgaugaaaa ccggaaccug     2220

agccagcuca uuuccgacuc uacgcugucg uucgauagcg cgaucaagac aauucagucc     2280

aaaucaaccg ucgaggacga cguguucgau gagaucaaga aacuugccgg aaguccugca     2340

auaaagcgug gcauucuuaa uucuaucaaa aucguugacg aacuggugca gauuaucggg     2400

uacccacccc aaaacauugu aauagagaug gcuagagaaa auaugacuac cgaggaggga     2460

cagaagaaag ccaagacaag gaagaccaaa uuagaaagcg cucucaagaa caucgaaaau     2520

uccguccugg aaaacgguaa agugccucau ucagaugagc agcuucaaag cgaaaagcug     2580

uauuuguacu aucuccagaa uggcaaagac auguacucuc uggauaagac ugggucccca     2640

acaccccuau aucgcgacca gcuggaucag uacgagguug accacauuau cccguauagu     2700

uuucucccua uugauagcau cgacaacaag gugcugacgc accgggagaa uaaccaaaga     2760

aaacuuaaua acauacccga uaaggaaacc gucgccaaua ugaaaccauu cugggagaag     2820

uuguacaacg caaaacugau cucucagacu aaguaccaga gguuaacaac cuccgaacga     2880

acaccugacg gagugcucac ugagucaaug aaggccggcu uuauugaacg ccagcuggua     2940

gagacccggc aaaucauuaa acauguggcu agaauccuug acaauagguu ccuggauacg     3000

aagauaauug cgcuaaaaag ucagcucauc accaacuuuc guaacacauu ccacaucgca     3060

aagauucgcg agcugaauga cuaucaucac gcccaugaug cuuacuuggc cguugucgug     3120

ggucagacuc uguuaaaagc auaucccaag cucgccccag aacugauaua cgggcaccac     3180

gcucauuuua accggcacga ggaaaauaag gccaccauca gaaaacaucu uuauagcaac     3240

auuaugaggu ucuuuaauaa ccccgacucg aaggugucca aagauaucug ggacugcaau     3300

cgagaucugc cuauuaucaa ggaggucaua uacaacucuc agguuaauuu cgugaaacgg     3360

acaaugauca agaagggcgc auuuuauaac caaaauccag ugggaaaauu caacaagcag     3420

auugcggcua auaaccgcua cccguugaaa acuaagguaa gcucacucga caccaguauc     3480

uacggcgggu auggacccau gaacuccgcc cugucuauua ucauaauugc agaaagauuc     3540

aaugagaaaa aggguaaaau cgagacgguc aaggaauuuc acgauaucuu cauuaucgac     3600

uacaagaaau uuaauaacaa cccuuuccag uuucuaaaug auacaagcga gaacggcuuc     3660

cugaagaaaa auaacauaaa uagggugcuu gccuuuuaug ggguuccaaa guacucccuc     3720

augcagaaaa acgacggaac ccggaugcug uucgaaucaa agagcaauuu gcacaaggcu     3780

acucaauuua aacugacaaa gacccagaac gaguuauucu uucauaugcg aagacuccug     3840

acuaaaagua aucuuaugga ucugaagucu gaguccgcca uuaaagaaag ccagaacuuc     3900

aucuugaagc acaaggagga auuugacaau auuucgaacc aacucucagc auucucucag     3960

aaaaugcugg gcgacaccac aagucuacgc aaucugauca aggguuauaa cgagaggaaa     4020

auaaaggaaa uugauuccag cgaggaggug aucaaauacu ucuaugacaa uuuuaucaag     4080

auguucucuu uugucaagag cggggcuccc aaagauauua acgacuucuu uaacaauaag     4140

uacacggugg cccguaugcg gccuaaacca gauaagaaau uccuuaacgc gacccucauc     4200

caucagucca uuacuggccu guacgaaaca agaauagacu ugucaaaguu aggagaggau     4260

uga                                                                   4263


<210>  3323
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3323
auggccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguug ugaccgauga cuacagagua cccgcauuua aguucaaagu gcucgguaac      120

acagagaagg agaaaauaaa gaagaaucuu aucggcucaa cuacguuugu cccugcccag      180

agugcgcaag ggaccagggu guuccggguu aaccgccgac guauugauag aaggaaucac      240

cggaucgcuu aucugcgcga cauuuuucag aaagagaucg aaaaggugga uaaaaacuuc      300

uacagaaggu uggacgagag cuuucggguc uuaggagaua aguccgagga acugcagaua      360

aaacagccau ucuuuggcga caaggagcua gaaacagccu aucauaagaa auacccgacu      420

aucuaucacc uccgcaagca ucuggcagau gccgacaaaa auucucccgu ggcugauauu      480

cgagagguau acauggcaau cucgcacauu cuuaaguaca gaggucauuu ccugaccuug      540

gacaggauca acccuaauaa cauaaauaug caaaacucau ggauugauuu uaucgaaagc      600

ugccaggagg uuuucgaccu cgagauuagu gacgaaucca aaacaaucgu ggccaucuuu      660

aagucuagcg auaaucggca ggagaaaguc aagaagauuc uguccuauuu ccagcaagaa      720

cugcucaaaa aggacaaauc aauauucaag caguuacugc agcuuuuguu ugggcugaaa      780

accaaguuca aggauuguuu ugagcuagaa gaggagccag accucaacuu caguaaagaa      840

aauuacgaug agaaccugga aaacuuucuu ggaagccugg aggaagacuu cucugaugug      900

uuugcuaagu ugaaagugcu ccgugacacu auccuguuau ccggcaugcu gacauauacc      960

ggcacgacuc gcgccagauu cucagcgacc auggucgaga gguacgagga acaccggaag     1020

gaucuccagc gauuuaagag cuucguuaag caaaaucuua gugagcagga cuaucuggau     1080

auuuuugggc gcaagacacc caacggauuc gacguggaca aagaaacuaa ggguuacgua     1140

ggcuauaucu cuaauaaaau gguguugacc aacccucaga agcagaaaac aauacaacag     1200

aauuuuuacg auuacauuuc cgggaagauc accggaaucg agggcgcaga auauuuccua     1260

aacaagauua gcgacgguac guuccugaga aaacucagga cuacagauaa uggggccguc     1320

ccaaaccaga uccacgcuua cgagcuggag aagauuauag accggcaagg agaagauuau     1380

cccuuucuuc uggagaauaa agacaaguug cucucgaucc ugaccuucaa aauuccuuac     1440

uaugugggcc cauuaucuaa gggcaacaau ucccgcuuug gguggaucaa aagaacuauc     1500

ucacaggaua uucuggacga uaacgacgaa gauacaagga auggaaagau acguccgugg     1560

aacuaccaga agcuaaucaa uauggacgag acccgggaug ccuucauuac gaaccucauc     1620

gguaacgaca uuauccugcu uaaugagaaa guuuugccca agcgaagucu gauauaugaa     1680

gaggugaugc uccagaacga acugaccaga gucaaauaca aggacaaaua cggcaagaca     1740

cacuuuuucg auagcgagcu uaggcaagaa aucauuaaua accuguuuaa gacuaauaac     1800

aaacgcgugu ccucugcaau guugcucgac uaucuggaga auuacaccaa cuuacaggcu     1860

guagagaucg uuagcgggau ugaaaaggga aaaucacuga auaguacacu aaagacuuau     1920

aacgaucuca aaaccaucuu cuccgaggaa cugcuugaca gcgagauaua ccagaaggaa     1980

uuggaggaga uugugaaggu caucacagug uuugaugaca aaaagucuau uaaaaauuau     2040

cugaucaagu acuucggcca ucucgaauuu cugaacgagg aacggauaaa ucaguuauca     2100

aaacugagau auaccgguug ggggagguac agugccaaac uuuugcucga uauccgcgac     2160

gaggauacug gcuucaaccu gaugcaauuu cuacgaaaug acgaugaaaa ccggaaccug     2220

agccagcuca uuuccgacuc uacgcugucg uucgauagcg cgaucaaguc cauucaguca     2280

aaaaguacag ucgaggacga cguguucgau gagaucaaga aacuugccgg aucuccugca     2340

auaaagcgug gcauuuugaa uagcaucaaa aucguugacg aacuggugca gauuaucggg     2400

uacccacccc aaaacauugu aauagagaug gcuagagaaa auaugaccac ugaggaggga     2460

cagaagaaag ccaagaccag gaagacaaaa uuagaauccg cucucaagaa caucgaaaau     2520

ucaguccugg aaaacgguaa agugccucau agcgaugagc agcuucaauc ugaaaagcug     2580

uauuuguacu aucuccagaa uggcaaagac auguacuccc uggauaagac cgggagucca     2640

acuccccuau aucgcgacca gcuggaucag uacgagguug accacauuau cccguauagc     2700

uuucucccua uugauucuau cgacaacaag gugcugacac accgggagaa uaaccaaaga     2760

aaacuuaaua acauacccga uaaggaaacg gucgccaaua ugaaaccauu cugggagaag     2820

uuguacaacg caaaacugau cucccagacc aaguaccaga gguuaacuac aucagaacga     2880

accccugacg gagugcucac agagaguaug aaggccggcu uuauugaacg ccagcuggua     2940

gagacucggc aaaucauuaa acauguggcu agaauccuug acaauagguu ccuggauacc     3000

aagauaauug cgcuaaaaag ccagcucauc acgaacuuuc guaacaccuu ccacaucgca     3060

aagauucgcg agcugaauga cuaucaucac gcccaugaug cuuacuuggc cguugucgug     3120

ggucagacac uguuaaaagc auaucccaag cucgccccag aacugauaua cgggcaccac     3180

gcucauuuua accggcacga ggaaaauaag gccacuauca gaaaacaucu uuauucgaac     3240

auuaugaggu ucuuuaauaa ccccgacucc aaggugucua aagauaucug ggacugcaau     3300

cgagaucugc cuauuaucaa ggaggucaua uacaacagcc agguuaauuu cgugaaacgg     3360

accaugauca agaagggcgc auuuuauaac caaaauccag ugggaaaauu caacaagcag     3420

auugcggcua auaaccgcua cccguugaaa acaaagguau caagucucga cacuuccauc     3480

uacggcgggu auggacccau gaacucugcc cugagcauua ucauaauugc agaaagauuc     3540

aaugagaaaa aggguaaaau cgagaccguc aaggaauuuc acgauaucuu cauuaucgac     3600

uacaagaaau uuaauaacaa cccuuuccag uuucuaaaug auacguccga gaacggcuuc     3660

cugaagaaaa auaacauaaa uagggugcuu gccuuuuaug ggguuccaaa guacucacuc     3720

augcagaaaa acgacggaac acggaugcug uucgaaagca agaguaauuu gcacaaggcu     3780

acccaauuua aacugacuaa gacacagaac gaguuauucu uucauaugcg aagacuccug     3840

accaaaucua aucuuaugga ucugaagucc gagagcgcca uuaaagaauc gcagaacuuc     3900

aucuugaagc acaaggagga auuugacaau auuucaaacc aacucucugc auucagucag     3960

aaaaugcugg gcgacacuac cucccuacgc aaucugauca aggguuauaa cgagaggaaa     4020

auaaaggaaa uugauagcuc ugaggaggug aucaaauacu ucuaugacaa uuuuaucaag     4080

auguucagcu uugucaaguc cggggcuccc aaagauauua acgacuucuu uaacaauaag     4140

uacacagugg cccguaugcg gccuaaacca gauaagaaau uccuuaacgc gacgcucauc     4200

caucagucaa uuaccggccu guacgaaacu agaauagacu ugaguaaguu aggagaggau     4260

uga                                                                   4263


<210>  3324
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3324
auggccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguug ugaccgagga uuacagagua cccgcauuua aguucaaagu gcucgguaac      120

acagaaaagg agaaaauaaa gaagaaucuu aucggcucaa cuacguuugu cccugcccag      180

agugcgcaag ggaccagggu guuccggguu aaccgccgac guauugacag aaggaaucac      240

cggaucgcuu aucugcgcga uauuuuucag aaagaaaucg agaagauaga caaaaacuuc      300

uacagaaggu uggaugaauc auuucgggug uuaggagaca aguccgagga uauccagauu      360

aaacagccau ucuuuggcaa uaaggagcug gaaacagccu aucauaagaa auacccgacu      420

aucuaucacc uacgcaagca ucucgcagac gccgauaaaa acucucccgu cgcugacauu      480

cgagaggugu acaugguacu gucgcacauc cuuaaguaca gaggucauuu ccugaccuug      540

gggaaaauag auccuaauaa cauuaauaug caaaacucau ggaucgacuu uauugaaagc      600

ugccaggaug cauucgaccu cgagaucagu gacgagucca agacaaucgc cgcuauuuuu      660

aaaucuagcg aaaauaggca ggagaagguu aagcagauac uguccuauuu ccaacaggaa      720

cugcucaaaa aggauaaauc aaucuucaag caguuacugc agcuuuuguu uggacugaaa      780

accaaguuca aggacuguuu ugagcuagaa gaggagccag aucucaacuu caguaaagaa      840

aauuacgacg agaaccugga aaaccuucug ggcgccuugg aggaagauuu uagcgacgug      900

uucgcgaagc ucaaaauucu gcgggauacu aucuuacugu cuuccaugcu cacauauacc      960

ggcgcaacgc acgcccguuu uucagcuacu auggucgagc gcuacgagga acacagaaag     1020

gaccuucaaa gguucaagag cuuugugaag cagaaucuga gugagcagga uuauuuggac     1080

auauucgggc ggaagacccc cacaggauuu gauguggaca aagaaacuaa ggguuacguc     1140

ggcuauauuu cuaacaaaau gguucuaacc aauccucaaa agcagaaaac aauccagcag     1200

aacuucuacg acuacaucuc cgggaagauu accggaaucg agggcgccga augcuuucug     1260

aauaagauua gcgaugguac guuccuccga aaacugcgca cuacagacaa cggggcaaua     1320

ccaaaucaaa uccaugcuua ugagcuugag aagauuaucg aaagacaggg aagggauuac     1380

cccuuccugu uggagaacaa agacaagcuc cugucgaucu uaaccuuuaa aauuccuuau     1440

uacgugggcc cacuggccaa gggcaauaac ucucgguucg cguggauaaa acgcacuaca     1500

ucccaggaug uacuagacga uaaugacgaa gauaccagaa acgggaagau caggccgugg     1560

aauuaucaca agcucauuaa cauggacgag acgcgugaug ccuuuaucau uaaucugauc     1620

ggaaacgaca uaauccuuuu gaacgagaaa gugcugccca agcggucacu cauuuacgaa     1680

gaggucaugc ugcagaauga acuuacccga gugaaauaua aggauaaaua cgguaagauc     1740

cauuucuuua acagugagcu gagacaagaa auuaucaaua acuuguucaa gacaaauagc     1800

aaaaggguuu ccucugcaau gcuccugagc uacuuagaga acuuuacuaa ucugcaggcu     1860

guggagauag ucucaggcau ugaaaagggg aaaagucuaa acuccacccu caagacauau     1920

aaugaccuga aaacuaucuu cagcgaggaa cuuuuggacu cugagauuua ccagaaggaa     1980

cuggaggaga ucauaaaggu gaucaccgua uuugaugaca aaaagucaau uaaaaacuau     2040

cucacaaagu acuucggaca ccuggaauuu uuaaaugagg agaaaaucaa ccagcugagu     2100

aaacuucgcu auaccggcug gggucgguac agcgggaagu ugcuccuggg cauuagagau     2160

gaagacacug gauucaaucu aaugcaauuu cugaggaacg augacgaaaa ucgcaaccuc     2220

acgcagcuga ucuccgauac aacccuuucu uucgacucgg ccauaaaagc uauucagagc     2280

aaguccacug uugaggauga cguguucgag gaaaucaaaa acuuggccgg cagcccugca     2340

aucaagcgag ggauucugaa uaguaucaaa auugucgaug aguuagugca gauaaucgga     2400

uauccacccc aaaacauuau cauugaaaug gcccgggaga auaugaccac agaggaaggu     2460

cagaagaaag cuaagacccg uaagucuaaa cucgagagcg cgcugaagaa caucgaagac     2520

uccguccuug agucaggcaa agugccucac agcgaugaac agcugcaauc ugagaaguug     2580

uaccucuacu aucugcagaa ugggaaagac auguacuccc uagacaagac uggaagucca     2640

acaccccugu auagagauca gcucgaccag uacgagguug aucauauaau cccguauagc     2700

uuucugccua uugacucuau cgauaacaag gugcuuacgc acagggaaaa uaaccaacgc     2760

aaauugaaua acauucccga caaggagacc guagcaaaua ugaaaccauu cugggaaaag     2820

cuguacaacg ccaaauuaau cucccagacu aaguaucagc ggcucacaac cucagagaga     2880

acaccugaug gcguccugac ugaaaguaug aaggcugguu uuauagagag gcagcuugug     2940

gagacccgac aaauuaucaa acauguugcc cgcauccugg acaaucgguu cagcgauacg     3000

aagauuauaa cccuaaaauc gcagcucgug acaaacuuua gaaauacuuu ccacaucgca     3060

aagauuaggg aacugaacga cuaccaucac gcccacgaug cuuacuuggc cgucguggua     3120

gggcagaccc uguuaaaagc auaucccaag cucgcgccag agcugaucua cggccaucac     3180

gcucauuuua accgucacga agagaauaag gccacacuuc gcaaacaccu guauuccaac     3240

auuaugcggu ucuuuaauaa ccccgacucu aaggugagca aagacaucug ggauuguaau     3300

agagacuugc cuauaguuaa ggaugucauc uacaacucac agauuaauuu cgugaaaagg     3360

acuaugauca agaagggagc auuuuauaac caaaauccag ugggcaaacu caacaagcag     3420

auugccgcua auaaccgaua cccgcugaaa accaagguca guucccuaga cacgucuauc     3480

uaugggggau acggucccau gaauagcgcc cuguccauaa uuaucaucgc agaacgguuc     3540

aacgagaaaa agggcaaaau ugagacaguu aaggaauuuc augauaucuu uauaauugac     3600

uacgagaagu ucaauaacaa uccuuuucag uuccuuaaca acgugucaga agagggguuu     3660

cucaaaaaga augaucugaa ccgcguguug gcuuucuaua gaaucagcaa auacagucug     3720

augcaaaaga uugacggaac caggauguua uuugagucua aauccaaucu ccacaaggua     3780

acucaguuca agcugacaaa aacccagaac gaacuuuucu uccauaugaa gcggcuguug     3840

acuaaaagca aucucaugga ucugaagucg gagucagcca ucaaagaauc ucagaacuuu     3900

auacaaaccc accagaauga guucgacaac auugcggauc gacuaaguaa uuauucccag     3960

aagaugcugg gcgaaacaaa gagccuucag acgcucauca acgguuacaa ugagagacca     4020

aucaaacaaa uugacaacaa ugaggaaacc auccgcuauu acuaugauaa cuuuauuaag     4080

auguucucuu ucgucaaaag cggggccccc aaggacauaa ucaacuuuuu caaugacaaa     4140

uacacugugc cuaggaugcg uccaaagccc gaucggaaga ugcuggacgc uacauugauu     4200

caucagucca ucaccggcuu auacgagaca agaaucgauc ugucaaaacu cggagaagac     4260

uga                                                                   4263


<210>  3325
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3325
augagcagaa agaacuacgu ggacgauuau gccaucuccc uggacauugg caaugcuucu       60

gucggguggu cagcauucac ccccaacuac aggcucguuc gggccaaagg acacgagcuu      120

auaggugugc gccuguuuga uccugcggac acagcugaaa gucgacguau ggccagaacu      180

acgaggcggc gcuauagcag aaggcggugg cgcuugcgau uacuggaugc acuauucgac      240

caggcccucu ccgagaucga uccaucuuuu cuggcuagaa ggaaguacuc guggguacau      300

ccggacgaug aaaauaacgc agacugcugg uauggcucag ugcuuuucga uagcaaugag      360

caagacaaac gguuuuacga aaaguauccc accauuuacc aucugcguaa agccuugaug      420

gaggaugaca gucagcacga cauccgcgag auuuaccucg cuauccacca uauggucaag      480

uauagaggga acuuccuggu ggaaggaaca cuggaguccu cuaaugccuu uaaggaagau      540

gagcucuuaa aacugcuugg caggauaacu cgguacgaga ugagcgaagg ugagcagaac      600

uccgacaucg aacaggauga cgagaauaag uugguugcgc cugcaaacgg gcaacuggcc      660

gaugcucuau gugccacccg aggaucacgc aguaugagag uggacaaugc acucgaagcu      720

cugagcgccg ucaacgaucu uucuagggag cagcgggcga uugugaaagc caucuucgca      780

ggccuggagg gcaauaaguu ggaccucgcu aaaauuuuug uauccaagga guucucaagc      840

gagaacaaga aaauccuggg gauauauuuu aauaagagug auuacgaaga gaaaugcguu      900

cagauugugg acucuggauu acuggaugac gaagaacgcg aguuccucga cagaaugcag      960

ggucaauaua acgccaucgc ucuuaagcag cuguugggca gguccacaag cgucucggau     1020

ucuaaaugug ccuccuacga cgcacaccgu gccaacugga aucuaauuaa gcugcagcuc     1080

cggaccaaag aaaacgagaa ggauaucaau gaaaacuaug ggauccuggu gggauggaag     1140

auugacucag gccagcgaaa aagugugaga ggugagagcg cuuacgaaaa uaugaggaag     1200

aaagcgaacg ucuuuuucaa gaaaaugaua gagacuuccg aucuuucuga gacagacaag     1260

aaucgccuga uccacgauau ugaagaggac aaguuguucc caauccaacg ggauagcgac     1320

aacgggguua uaccccauca gcuccaccag aaugaacuga aacaaauuau caagaaacag     1380

ggaaaguacu auccuuuucu ccuggaugca uucgagaaag acggcaagca gaucaacaag     1440

auugagggcc uacucaccuu uagagugcca uacuucguag ggccccuggu ggucccugaa     1500

gaucuucaga aaucagacaa uagugagaac cauuggaugg ugaggaagaa aaagggagaa     1560

aucacgccau ggaauuuuga cgagaugguu gauaaagacg ccuccggucg caaguucauu     1620

gaacgauugg ugggcacuga uagcuaucug cucggggagc cgacccugcc caagaacucu     1680

cuucuguacc aagaguauga agucuugaau gagcucaaca acgugcggcu gucaguacgu     1740

acaggaaauc acuggaacga caaaagaagg augcgcuuag gccgggaaga gaagacucug     1800

cuaugccaga gacucuuuau gaaaggucag accguuacaa agaggaccgc ugaaaaucug     1860

cuucgaaaag aguacgggcg cacguaugag uugaguggcc ugagcgauga auccaaguuc     1920

acuucuucgc ucagcacaua cggaaaaaug ugucggauau uuggcgagaa guacgugaac     1980

gaacacagag accugaugga gaagaucguc gaauuacaga ccguguucga ggauaaagag     2040

acucugcuuc aucaauugag gcagcucgaa gggauuuccg aggccgacug cgcacugcua     2100

gucaauacac acuauaccgg auggggucgu cugucacgca agcuccugac gaccaaagcc     2160

ggcgaaugua agaucaguga ugacuuugcu ccucggaaac auucuaucau ugagauaaug     2220

agagccgagg auaggaaccu uauggaaauc auuacagaca agcaguuggg guucagcgau     2280

uggaucgagc aggaaaaucu gggagcagag aacggcuccu cauuaaugga agugguugac     2340

gaccuccgag ugagcccaaa gguaaaacgg gguauuaucc aaucuauacg ccugaucgau     2400

gacauuucca aggcggucgg gaaaagaccc aguaggaucu uucuugagcu ggcugaugac     2460

auucagccua gcggccggac uaucucucga aaguccagau ugcaggaucu cuaccgcaau     2520

gccaaccugg gaaaagaguu caagggcaua gcagacgaac uaaaugccug cucagauaag     2580

gaccugcagg augacagguu auuccuguau uacacccaac uugggaaaga uauguauaca     2640

ggagaggaau uggaccugga ccguuuaagu agcgcuuacg auauugacca caucauucca     2700

caggccguga cucagaacga uucgaucgac aaucggguuc ucguggcaag agcugagaac     2760

gccaggaaga ccgauuccuu uacauauaug ccccaaauag cggaccgcau gcgaaauuuc     2820

uggcagaucc ugcuugauaa cggucugauu ucuagaguca aauuugaacg gcuaaccagg     2880

cagaacgagu ucagcgagcg cgaaaaggag cguuuugugc agagaucacu cguagaaacu     2940

cggcaaauca ugaaaaaugu ggccacgcug augaggcagc gcuacggcaa cagugcugca     3000

guuauugggu ugaaugccga gcugacaaag gaaaugcauc gauacuuagg auucucccac     3060

aagaacagag acaucaauga uuaucaccau gcucaggacg cccucugugu cggcauagca     3120

ggucaguuug cggccaaccg gggguucuuu gcugauggcg aggugucuga cggagcccaa     3180

aauagcuaca accaguaucu gagggauuac cuucgcggcu auagagagaa acuguccgca     3240

gaagacagga agcaggggcg ggcuuucgga uuuauugugg gcucaaugcg uagccaggac     3300

gagcaaaaac gcgucaaucc gcgaaccggu gaaguugugu ggagugagga ggauaaggac     3360

uacuugagaa aagugaugaa cuauaggaag augcucguaa cucagaaagu cggggaugac     3420

uucggagccc uguacgauga aacccgguac gcagccacag acccuaaggg caucaagggu     3480

auccccuuug auggcgcuaa acaggacacc ucucuauaug ggggauucuc cagcgcgaag     3540

ccagccuacg cagugcugau ugagucgaaa ggcaagacuc gccuuguuaa ugugacaaug     3600

caagaauauu cacuccuggg ggauagaccu ucugacgaug aguugaggaa aguccuggcu     3660

aagaagaaaa gugaauacgc caaggccaac aucuuacucc ggcacgugcc caaaaugcag     3720

cugauucgau auggaggcgg ucuuaugguu auaaaguccg cuggggagcu gaauaacgca     3780

cagcaguugu ggcucccaua cgaggaauau ugcuacuucg acgaucugag ccaaggaaaa     3840

ggcucucuag agaaggacga ccugaagaaa cuucucgaua gcauccuggg uuccgugcag     3900

uguuuguacc ccuggcauag auuuacggaa gaggaauuag ccgaccugca cguagcguuc     3960

gauaagcucc cugaggacga gaaaaagaau gucauuaccg gcaucguguc agcucugcac     4020

gccgaugcaa aaacugccaa ccuuaguauu guggggauga caggaagcug gcgcaggaug     4080

aacaauaagu cuggcuauac cuuuuccgac gaagaugagu ucaucuuuca gucaccaagu     4140

gggcuguucg aaaagcgggu cacaguugga gaguugaaac guaaggcuaa aaaggaagug     4200

aacagcaaau acagaacuaa ugagaagagg cuaccgaccc uguccggcgc aucucagccc     4260

uga                                                                   4263


<210>  3326
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3326
auggccaagc ccaaagacua caccaucggc cuggauauug ggacaaacag cgugggaugg       60

gucguuacug acgaucagaa uaacauacuc agaaucaagg guaaaaaggc uauuggcgca      120

aggcuuuucg ccgaggggaa aguggcggcu gaacggcgcu ccuuucgaac gacccguaga      180

aggaucucuc ggcgcagaug gaggauuaag cuguuggagg aauuauucga cgaggaaauc      240

gccaagguag auccuucauu uuucgcacgg cugcacgaga guuggauaag cccaaaagac      300

aagcgcaaac gauauuccgc caucguguuu ccgucucccg aggaagauaa gaaauuccau      360

gagucguacc cuacaauuua ucaccuaaga gacaagcuca ugaaggauga ccaaaaacau      420

gauaucaggg aaauuuacau cgcuguccac cagaugauaa aggugcgggg aaauuuucug      480

caugacgagu caguugagac uuaucguagc ggcaugaguu cccuuggugg gcgcucugaa      540

agaaccauuc ugagcgugca gacauuggag gaacucaacg aucuguucgc agagaaugaa      600

ggaaccgagg aggucgaacu gaacguggcc uccgcugagc agaucaauga cauucucacu      660

ggcgaccacu uaaacgccga uucacaaaaa gaaaucagua aucugcuuuu gccaagcucu      720

uuucccuccu ucgacgauaa ggcgaaagag aagcagguaa agaagcugau caacaauguu      780

gcaacaaaca uuucaaaggc cuggcuaggc uacaaagcug acuuuagcac cauacucaau      840

cuggccaagg uggauaaaga ccagaagaaa aucuucgcau uugcccuuca agggggagau      900

gaagaggaca agguccagga gcuggaaagu uugcucgagc agucucagac ggauauugug      960

gacaggcuga ucgaaauacg gcacgcuauu guguuauccg agaucguccc uguugguaug     1020

acucugagcg aagcgaugau cgauaaguau gaccagcaua aagaggaccu cauuacccuu     1080

aaggccguga uccgaaacac aaaagauaag aaaaaggcag cuaagcugca agccauuuac     1140

gacuuguacg uaaauaaccg ccacgcugau cuagccaaag caaugaagcu gacuggcaua     1200

aagaagagau cggagcuccu ggacccagag gaacuuaaaa aggccaucuc uucccuguug     1260

gaugacucac ccgaagcugg ggagauuaag cagaggcucg agggaaaaac cuuccugccu     1320

uuacagcgga guaacaauaa cggcgugauc ccaaaucaac ugcaucaggu cgaacuagau     1380

gagaucauua agaaacagag caaguauuac ccguuccucg cggaaaagaa ccccgacgag     1440

uccgaagagg cacagaagaa agccccuaca aagcuggaug cucuuuugac cuuucgcgug     1500

ccauauuacg uuggucccau gauaacgaag gaggaacaag aggcccagga cgggcacucu     1560

uucgcaugga uggugagaag ggauccucag gaccacgaag ccaucacucc auggaauuuu     1620

gagaaaaagg ucgauaaaau ggcuagcgcc acacaguuca uuaagcguau gaccacuaaa     1680

gacacauauc ugcucggaga agacgugcug cccgcaucaa gucuuaagua ccaacuguuu     1740

accguauuga acgagcucaa uaaccugcgg guuaauggca agaaauuaac guccgaugag     1800

aaggaacagg ugaucgaggg ccuguucaaa aagcagaaaa ccgucaaggc ggacaaguuu     1860

gugaaauauu ggcaggcuau ucauaucggg gccgauauaa aggucaaagg acuaagcgac     1920

ccgucuaagu ucaacucaac aaugaguacu uacaucgauu uuaaaaagau uuucggugac     1980

caacucaaug augcaaaccg acagaaugac uuugaaaaga ucauugagua cagcaccauc     2040

uucgaagaua gaaaaauacu ggaggacaaa cuuagggccg auuuugcuug guuguccgag     2100

gaacagauua aacagcugug ccgccuccgg cugcaaggcu gggggagauu aucugacaag     2160

cugcuuacaa gguugacuga ugccgacgga cagaacgugc ucgagcgccu guggaacucg     2220

aaugacaacu ucguucagau cgugagcgau ccuaauauua aagcaaagau cgaacaagag     2280

aaccagaaac uacugcgaga cggccagucc gaugacgcug uagagucaau acucgaugac     2340

gccuauacca guccccagaa uaagaaggcg auccggcaag ucaugcgugu gguucacgau     2400

auugugaaag ccugugacgg uaacguccca gcuaaguucg caaucgaauu ugccagaucu     2460

caggaggaug acccuaaaag gacaaccgcu cgcgugaauc agaugcagaa gauuuacgau     2520

caaaucagcg acgaaguagu gucccagggg guuaaagagc agcugucagg caugaaaagc     2580

cuuaaggacc gguauuacuu guauuucaug cagggaggca gagaugccua cacuggggca     2640

aaacugaaca uagacagguu aucugauuau gacauugauc auaucaugcc ccaauccuuu     2700

gucaaggacg auagucucga caaucgagug cugguggcgc gcgccgucaa caaucagaaa     2760

agcgauaagg uuccagcuuu ccuuuuugga aacaaagugg uggccgaccu ggguaucacg     2820

guacgggaaa ugugggauaa guggcagaag uugggcauga uuucuaaaag aaagcucucc     2880

aaucugcuaa cagaccccga ugcacugacc gaguacaggg cucaaggguu cauccgucgc     2940

cagcucgucg agacuucaca ggugauuaaa cugaccgcca caauacuuca gagugaauuu     3000

ccugacagca agaucauuga gguuccagca aaauacaacu cgaucgugcg gaagcaauuc     3060

gacuuguaua aguccagaga agucaaugau uuucaccaug ccauugacgc uuaccugucu     3120

accaucgugg gaaacuauuu auaccagguu uauccgaauc ucaggcgaau guucguguac     3180

ggcgaguuua aaaaguucag cucaaacgcg gaagagagug cccacgauau ggcacggcgc     3240

guaaaaucca ugaacuuucu ggacgaucuu cugagaggua cucacgggga caauauauau     3300

ugcaggucua caggcgagau cgucuucaac cggaaugaua uuaucagcaa gcuaaaacag     3360

gcuuacuccu ucaagcagau gcucgugacg caagaagugu uuacccgaaa gucagcccug     3420

uucgaccaga cugucuaccc cagcccugag agagauagua aaaagcgcuc uggauugauu     3480

ccaaggaaaa agggcaugga cacagaaauc uaugggggau acuccgguaa caaagaugcc     3540

uauuuuguuc uggcugaggc agugaaggaa aaaggccaua ccuuacagau aguaggggug     3600

cccauucgug cccucaauac acugaagaac agcgcgaauu acucggagaa acuucuggag     3660

aucaucaaac cucaggucau guucaacaag aaaaauggaa agccaaagaa gggcgugaac     3720

gacguucgga uuuugaugga uaauauaccc uauagacaac cggugcucga agguggggcu     3780

cuguacaugc uaggcucauc uacuuauaag uacagucuga aacagaucac ccuuucccag     3840

gagagcauga aguacauucu cgacuauauc gacgauccuu cuuuuaacaa acacauuaug     3900

gucaauaucg accaucagga ugaaaagaaa ugucuguuga gcguguacga cgagauacug     3960

gauaagaugg acaaguauuu accacucuuc gauaucaacg gauuuaggaa aaagcugcac     4020

gacggccgcg agaaauucgu uaaacuuccc guggaaaaac aagccgaggu cguaaaggau     4080

auucugcgag gguugcacgc aaauuccgaa augacgaagc cuucagaccu cggacugacc     4140

acagcccuag gcuuuaugca ggcucccgau gguaucaaaa uuagugacga cgcaguguuc     4200

aucuaccaga gcccaacugg gcuguuugag agacggguua aggugucuga ucuucucaaa     4260

uga                                                                   4263


<210>  3327
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3327
augaccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugccguug ugacagagga uuacagagua cccgcuuuua aguucaaagu gcucgguaac      120

acugaaaagg agaaaauaaa gaagaaucuu aucggcucaa cgaccuuugu cagugcacag      180

ccugccaaag ggacaagggu guuccggguu aaccgccgac guauugacag aaggaaucac      240

cggaucacuu aucugcgcga uauuuuucaa aaggaaaucg agaaagugga caagaacuuc      300

uacagaaggu uggaugaauc uuuucggguc uuaggagaca aauccgagga ucugcagaua      360

aagcagccau ucuuuggcga caaggagcua gaaaccgcgu aucauaaaaa guacccgaca      420

aucuaucacc uccgcaaaca ucuggcugau gccgacaaga auagccccgu ggcagauauu      480

cgagagguau acauggccau cucgcacauu cuuaaauaca gaggucauuu ccugaccuug      540

gacaagauca acccuaauaa cauaaauaug cagaacucau ggauugauuu uaucgaaagc      600

ugccaagagg uuuucgaccu cgagauuagu gacgaaucca aaaauaucgc ugauaucuuu      660

aagucuagcg agaacaggca ggaaaaggug aaaaagauuc ugccauauuu ccagcaggag      720

cugcucaaaa aggacaaauc cauauucaag caauuacugc agcuuuuguu ugggcugaag      780

acuaaauuca aggauuguuu ugaacuagag gaggaacccg accucaauuu cucaaaagag      840

aacuacgaug aaaaccugga gaauuuucuu ggaagucugg aagaggacuu cagcgauguc      900

uuugcaaagu ugaaagugcu ccgggacaca auccuguuau cuggcaugcu gaccuauacg      960

ggcgccacuc acgcucguuu cuccgccacc augguggagc gcuacgaaga acacagaaag     1020

gaucuccaga gguuuaaguu cuuuauuaaa cagaaccuuu cagaacaaga cuaucuggau     1080

aucuucgggc ggaagacaca gaauggauuu gacgucgaca aagagacuaa ggguuacguu     1140

ggcuauauaa ccaacaaaau gguguugaca aauccucaga agcaaaagac cauucagcag     1200

aacuucuacg auuacaucag cgggaaaauc acgggaauug aaggcgcgga guauuuccua     1260

aauaagauca gugacgguac uuuucugcga aaacuccgca caucugauaa cggggcaauu     1320

ccaaaucaga uacaugccua cgagcuggaa aagaucauug agagacaagg aaaagacuau     1380

cccuuccuuc uggaaaacaa ggauaaauug cucuccaucc ugaccuuuaa gaucccuuac     1440

uauguaggcc cauuagcuaa gggcagcaau ucgagguucg ccuggauuaa acgggcaacu     1500

ucuuccgaca uacuggauga caacgaugag gacacacgca augggaagau cagaccgugg     1560

aacuaccaga aacuaauuaa uauggaugag accagggacg cuuuuaucac gaaccucauu     1620

ggaaacgaua ucauacugcu uaaugaaaag guguugccca aacguucacu gaucuaugag     1680

gaagucaugc uccagaacga gcugacccgg gugaaguaca aggacaaaua cgguaaggcc     1740

cacuucuuug acagugaacu ucgacagaau auuaucaacg gccuguucaa aaauaacagc     1800

aagagaguua augcgaaauc cuugauuaag uaucucucug auaaccauaa ggaccugaau     1860

gccaucgaga uagugagcgg ggucgagaaa ggaaagucau uuaacaguac auuaaaaacu     1920

uacaaugauc ugaagaccau uuucuccgag gagcuacucg acagcgaaau cuaucaaaaa     1980

gagcuggaag agauuaucaa ggugauaaca guauuugaug acaagaaauc uaucaagaac     2040

uaccuuacua aauucuuugg ccacuuggag auucuggaug aagagaagau caaucagcuc     2100

ucaaaacuga gguauagugg uugggggcgc uacagcgcaa aguuacugcu ugacauucgg     2160

gaugaagaca ccggcuucaa cuugcuccag uuccugagaa augaugagga aaacaggaac     2220

cuaacaaaac ugaucuccga caauacccuc ucuuuugagc cuaagauaaa ggauauucag     2280

ucgaaaagca cuaucgagga cgacaucuuc gaugaaauua agaaacuggc uggaucccca     2340

gccaucaagc gcggcauucu uaacucaaua aaaaucguug acgaguuggu gcaaauuauc     2400

ggguaucccc cucacaauau ugucaucgaa auggcucgag agaacaugac gacagaggaa     2460

ggacagaaga aggccaaaac ccggaagacu aaacuggaga gugcauuaaa gaauauagaa     2520

aacucucucc uggagaaugg uaaagugcca cauagcgaug aacagcuuca guccgagaag     2580

cuguacuugu acuaucucca aaacggcaag gacauguaca cccuggauaa aacaggguca     2640

cccgccccgc uauaucugga ccagcucgau caguacgagg ucgaccacau cauuccuuau     2700

agcuuucugc ccaucgauuc uauugacaau aaggugcuua cccaucguga aaacaaucaa     2760

cagaaauuga acaauauccc agauaaggag acuguugcua acaugaaacc uuucugggaa     2820

aagcuguaca augcgaaguu aauaucccag acaaaauauc agagacucac gaccagugag     2880

aggacucccg acggagugcu gacagaaagc augaaggcag gcuuuauuga gcgccaacuu     2940

guagagaccc ggcagaucau caaacacguc gccaggauuc uggacaacag auucucugau     3000

acaaagauaa ucacucuaaa aucccagcuc auuaccaacu uucgaaauac guuccauauc     3060

gcuaagauuc gcgaacugaa cgacuaccac cacgcccaug augcauacuu ggccgugguu     3120

gugggucaga cccuguuaaa ggucuaucca aaacucgcuc ccgagcugau cuacgggcac     3180

caugcccacu uuaaucggca cgaagagaac aaggcaacac uuagaaaaca ucuguauuca     3240

aauauaauga gguucuuuaa caauccugac aguaagguga gcaaagauau cugggacugc     3300

aaccgugauu ugccaauuau caaggacgua auuuacaauu cgcaaaucaa cuucgugaaa     3360

cgcacuauga uaaagaaggg cgcguucuau aaucagaacc cgguuggaaa auuuaauaag     3420

cagcucgcug ccaacaaucg guacccccug aaaaccaagg cacuaugucu ggauacaucc     3480

auuuauggcg gguacggacc uaugaacucu gcccuuagca ucaucauuau cgcugaaaga     3540

uucaaugaga aaaaggguaa gauagagacu gucaaagaau uucacgacau uuucaucauu     3600

gauuacgaga aguuuaacaa caauccauuc caguuucuca acgacaccuc agaaaauggc     3660

uuccugaaaa agaacaauau caacagggug uugggguuuu aucgaauacc caaauacagu     3720

cugaugcaaa agauugaugg aacgcggaug uuauucgagu ccaagucuaa ucuccauaaa     3780

gccacacagu uuaagcugac caaaacucag aacgagcuuu ucuuucacau gaagcgccug     3840

uugacaaaaa gcaaucucau ggaccugaag uccaagucag caaucaaaga aagccaaaac     3900

uucauccuaa agcauaaaga ggaguucgac aauauuagua accagcuguc ugcuuuuucc     3960

cagaagaugc uuggcaauac cacuagccuc aaaaaccuga ucaaggguua uaaugaaaga     4020

aaaauuaagg aaauagauau cagggacgag accauuaagu acuucuauga uaacuuuauc     4080

aaaauguucu cguuugugaa gucaggggcc ccuaaagaca ucaacgauuu cuuugacaau     4140

aagugcacag ucgcgcggau gcgaccaaaa cccgauaaga aguuguuaaa cgccacgcug     4200

auucaccagu cuauaaccgg ccucuacgag acuagaaucg accugaguaa acuuggagaa     4260

gauuga                                                                4266


<210>  3328
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3328
augaagaaaa uccugggccu cgaccuuggg cccaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acgagaauga aaaguccucu auaaucaaaa uugguucaag agucauccac      120

uacggcgaua acuugguuaa gguggacaaa agugggaaga uuagcgcauc caugaccccu      180

gaggaagauu ucucuucggg aaagggcuua ucaccaaaug ccgguaggac aaaacagcgg      240

agcgcgcgcc gaaaccugca acguuauaag cagagaagga auaaccuaau cgagauacuc      300

aaagcucaua gucugaucga caauaacuuu auuuucuccg aggaagggaa ugagacuacg      360

uuuaagaccu acaaaaaccg ggcccuugca gccacaaaug aaaucucucu ggaggauuuc      420

auuaagguau uguuuaugau caacaagaaa cgcggauaua agagcuccag aaaagcuaag      480

ggcgacgagg auggccagcu cauagacggg auggcaauug ccauccagcu guacgaacac      540

aaucaaacuc cgggacaguu cguguauaac cugcucaaaa acgguaagaa aaauauuccc      600

gauuuuuaca ggucagacuu acagaccgag uucgaucgga ucuggaacuu ucagaaggcu      660

uuccaucaag aaagucugag cgacggcauc ugcgagaaac uugucgggca gaaaaagaau      720

gugacaucug ccauuuuguu uaaagaauuu aagauagagu cccuggagau caaaggaaag      780

ugggaugaaa agcgcuuuuu ccgauaugag cuaagaaaca aagcgguuca gcaggaacuc      840

aagcugaaug agauugcaga augucuugug gagaucaacg gcgagauauc aaauagcagu      900

gguuaccugg gggccauuuc ugacaggucc aaagaauugg ucuuccaaaa gaucaccgug      960

ggacaguacc ucuauaaaca gaucgcugcc aacccuaaua cuagccugaa gaagcaaauu     1020

uucuaccggc aggacuauuu agaugaguuc aacacacugu gggaaaccca ggcaaaauac     1080

cacaaggagc ucacggacga acuuaaaacu cagauccgug auguaauuau auuuuaucaa     1140

cgcagacuga agucgcagaa aggcuugauc ucuuucugcg aguuugaguc caagcagauu     1200

gaaguuuucg uggacggcaa gaaaucaauc aagaccaggg ggaugcgggu caucccaaaa     1260

aguagccccc uauuucagga guucaagauu uggcaaauac ugaauaacau cgaaauuaca     1320

gaucucaaaa cuaaggaguc ccgagagcug accgacgaug aacgccagcu ucuguuugag     1380

gaauugaaua ucagaccuaa aauuucuaag aaggaggcuc ucaaaauccu gacagaaaag     1440

ccagcccagu acgacuuaaa cuauaaagau auagagggaa auaagaccaa cgcgaaauuc     1500

uacgaggccu acaacaagau cauucaucug agcggucacg acgaaaucga uuuuacggac     1560

aagacuucag acauuaauga guaucuaaaa cagaucuuca gucuccuggg cauaaagccc     1620

gaaauucuug auuuugacuc cagcuugaaa gaugaggacu ucgaaaagca acugcacuuc     1680

cagcucuggc aucugcuuua cucuuuugag ggggauaaca caaaaaccgg agacgagaag     1740

cuguugcaga agcucaauac uaaauucggc uuuccugugg aauaugcaaa ggugaucuca     1800

aacauugcuc ugccagauga cuacgguagu uuaagcgcca aagcuaucag gaagauacug     1860

ccgcaccuaa aagauggguu cuccuauggc ggacggaagu cucgccccga cgagccuucg     1920

gccugugaau acgcaggcua uagacauagc aaggccuccc ucacaaaaga ugagaaggaa     1980

aauaaaaccu acaaggacaa acuggagauc cuuccaaaga acucauugag gaaucccauu     2040

gucgagaaaa uccugaacca gacgauuaau guugugaacg cuguaaucgg ggauuacgga     2100

aagccugacg aaauacgucu cgagcuggcg cgggaauuaa agaaaagugc agaggagcga     2160

caagacgcca ccgauucuau uagcaaggcu acagccaaac acgaaaagua uagaaaagug     2220

cugcagacug aguuuggucu uaagcacauc uccaggaaug acuugauccg cuacaagcuc     2280

uaucuggaac ugaaaaacaa uggcuucaag acccuauacu cagauacaua uauugcacca     2340

uacgagaucu uuagcaaaaa guucgacauu gaacauauac uccccaaagc caagcuguuu     2400

gaugacucuu ucuccaacaa gacucuugag uugaaaagug ucaaucugaa gaaaggggau     2460

augaccgcuu augacuuugu gaaggaggaa uacggaacag agggcuuagc caaauacgaa     2520

cagcggguuc aagaucucuu ccugaacaag aagagaaacg aggaccuuga ucaggaaaau     2580

gagagcagga ucaccgacga gaaaauuucu aagacuaaac gcgauaagcu guugaugccg     2640

gaaaacaaaa ucccuacggg uuuuauugac cgagagcucc gggacuccca guauaucgca     2700

aagaaggcga aaacaaugau ggaagauaua ugcaaggacg uggucaccac uaccggguca     2760

gugacagauc gucugagaga ggacuggcag cuaaucgaua uuaugcaaga acugaauugg     2820

cccaaauacg cugcccucgg ccugaccgag gaguucacua acgaagaggg aaauguaauc     2880

aggaagauua aagacuggac aaagcgcaac gaucaccggc aucacgcaau ggacgccauc     2940

acgauagcuu ucaccaaaag aagucauauu caguaucuua auaacuugag cgccaggucg     3000

gauaaguccu cuagcaucua cggcaucgaa cagaaggagc uguaucgaga caaaaauaac     3060

aaguuacgcu uugcaccacc uauucccaua aaugaguucc gggcugaagc caaaaugcag     3120

cucgagaaca cucugaucuc aauuaaggcg caaaauaaag uugugacaaa gaacaagaau     3180

aaaaucagug ggcagaagaa acuucaggaa acccugacac caagaggaca gcuacaccuc     3240

gagacuauuu acgguaagau caaccaauau cagaccgccg aaauaaaagu cggcgggacg     3300

cuggauuccg aguugaucaa caagguggcu aauaagaaaa ccagggaggc acuguuaaac     3360

cgucucgccg aauuuggaaa ugaccccaag aaggcuuuca cagucaagaa cacuauugac     3420

aaaaauccua ucuaccugga uaagcaccag ucuuacaaga uuccagagaa agugaagauc     3480

guuuggcaag aaccggugua uaccauacgc aaaagcauua cacccgaccu uaagcuggau     3540

aaaaucaucg acccuaagau uaaagccuug cucgaguccc ggcugaagga auguaacggc     3600

gaugcaaagc uagcguuuuc aaaucuggcc gagaacccaa ucuggcuuaa uaaagagaag     3660

gguauacaga uuaaaagagu aacuaucacc gggauuagca acgcugccag ucuccaugac     3720

aagaaagauc ugaauggcaa gcccaucuug gacgaaaagg gaaacaaaau aucugucgau     3780

uucgugaaua cgggcaacaa ucaccauguu gcaauuuaca aggacgagaa cgggaaucug     3840

cacgauaacg uggucacauu uuucgaagcu guggagaggg uaaaccagaa uuuaccugug     3900

guugacaaac ucuuuaagca gaaagaagga uggcaauucc uguuuaccau gaagcagaac     3960

gaguauuucg ucuucccaaa ugagaucacu gccuuuaacc ccaaugaguu cgaucuucug     4020

aacccggaca auuacuauuu gaucuccccu aaccucuuuc gagugcagac aaugagcaag     4080

gugauguacg gcaauaacac ccugcgggac uauaaauucc gccaccaucu agaaacuacc     4140

cugcaggaua uuaaggaacu uagagacauc gucuuuaaac aauucaaguc gcucucaauu     4200

uuugauaaaa uaguuaaggu gaggaucaau caccugggua agauugugaa aacaggggag     4260

uacuga                                                                4266


<210>  3329
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3329
augaaggaca uccugggccu cgaucuuggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augacaacga acaauccgag auaguugaug uggguguaag agugaauccc      120

cugacaacug acgaaaagac gaacuucgag aaaggcaagc cuaucucuac caaugcagau      180

aggacauuga aacggucagc ccgccgaaac uuacagcguu acaagcugag aaggaaggaa      240

cuaauugagc uccugaaaga gcaccagcuu aucaguacug acaccauucu gacagaaaau      300

gggaagggaa ccacuuuuca gacauuggag cuccgggcga aaagcgcuaa ggagaaaauc      360

ucccuggagg agcuggcccg cgucuuccuc accauaaaca agaagagagg cuauaaaucu      420

ucgaggaagg ccaaaaauga agaugagggu caauuaaucg acgggaugga aauugcaaag      480

cggcuguacg aggaaaaccu uacgacugga caguugucac ugcagcuacu caaagagggc      540

aagaaaagcc ugccagauua uuaccgcagu gaccuucagu uugagcugga uaaggugugg      600

aauuuccaaa agcaguuuca uucccacauc uugaccgacg aguucuaugc ucagcucaaa      660

ggcaaggggc agcgagcaac aucugcccug uuuuuagcua aauaccaaau uuauacugcc      720

gaaaacaagg gaagcagaga agauaaaaag cugcaggcgu accaguggag gguugacgca      780

cucuccaagg agcuuacccg ggaacaacug gccuucguga ucacagagau aaauaacaau      840

uugaacaacu caagugguua ccuaggcgcu auuagcgacc guucuaaaga gcuguauuuu      900

aauaacgaaa ccgucgggga guaccucugg aagcagaucg ccgcaaaucc gcauacgucc      960

cugaaaaacc agguguucua ucgccaggau uaccuugacg aauuugagca aauuuggaag     1020

acucagucag cuuucuaucc cgaaauccug acagagagcu ugaaaaagga gcucagagau     1080

guaguuaucu uuuaccagag gaagcugaaa agucagaagu cuuuaguguc cuucugcgag     1140

uucgaaaaua aagaaauuga gauaaagaaa gaggacggaa ccagcacuaa gaaggucauc     1200

ggccugaaag uggccccuaa gucgucucca cuauuucaag aguucaaaau uuggcagaac     1260

cucaauaacg ugcugauccg gaagaaaggu uccaagacca aaaaggcggc caagaaucag     1320

caggcagucc uuuuugaaga agagaaaaca auaucagaau uggaucugga ggcuaagcaa     1380

gcccucuuca acgagcugaa ucuuaaaggg aaccugaagg cugacuauug uuuggaaauu     1440

cucggauaca gucccaaaga gugggaaaug aauuacagcg uucuggaggg caaccgaacg     1500

aauaaggccu uauaugaagc auaccugaag aucaccgauc uagagggcua ugacgccaaa     1560

aaccaccuca aggugaaacu gaacaaggau gagguagaac uugacgauuu gaaagugccu     1620

gcuuccgaga ucaaggacau gguccaggcg auuuuuaagg aagcagggau caauacagac     1680

auucugcauu ucaacgccga gcucgaugga aaagacuuug aaaaucaggc uucuuaccag     1740

cuguggcacu uacuguauag cuacgagggu gaugacucaa agaguggcaa cgagcuuuug     1800

uauaaacucc uggaaacuaa guucggguuu aaaaaggagu acgcccaaau acuagcaaau     1860

gugucccuga gcgaugacua cggaucucuc ucaaccaagg ccaugcgcaa aauccaccca     1920

uauauuaagg aaaacaaauu caguacagcu ugcgagcugg ccggcuacag acauagcaag     1980

cacucccuua cuaaagagga aaaugauaac aggguuuugc gggacucucu ggagguguua     2040

aagaaaaauu cgcuccgcca gcccgucgug gaaaagaucc ugaaccagau gaucaaucuu     2100

guaaacgcac uguugaagaa auauagaacc gagaauccua acuuuaaguu cgaugaaauu     2160

aggauagagc ucgcgcguga gcugaaaaag aaugcugagg aacgggccaa caugacagca     2220

aacaucaaua aagccaagac cgaacaugag aagauuauca aaauucuaaa gaacgaauuu     2280

ggucugccaa auccgacucg aaacgacauc auaagauaca ggcucuauga ggagcugaaa     2340

gcuaaugggu acaaggaucu uuauacgaac aucaaaauuc cccaagaaaa guuguucaca     2400

aaggagaucg acauugaaca caucauaccu aaaagccgcg uuuucgauga cuccuuuuca     2460

aauaagaccc ugaguuuccg gaaaacuaac uuagacaagg gcgagagaac cgccuuugau     2520

uacauggaau cuaaauucgg agcagacaag cucgaggagu acguggcuag ggucgaagau     2580

cuguauaaga auaacauuau cagcaaagcc aaguaccaga aacuucugaa gaaagagucc     2640

gaaauuggcg acggguuugu ggagcgcgau cuacgagaga cacaguauau cgcgaagaag     2700

gccaaagaaa uacuccugca gaucacccca aaugucuugu caacuagcgg agguauuaca     2760

gaccggcugc gugaggauug ggacuuaauc aacgugauga aggaacucaa ucugcccaaa     2820

uacagagcuc uuggccugac ggagauggaa gauaggaagu augggcaaca gguugaggug     2880

auuaucgacu ggaccaaacg caacgaucac cggcaucacg caauggacgc cuugacugua     2940

gcuuucacaa agcauaauca cauacaguac cucaacuauc ugaacgccag gaaaaaugag     3000

aagcauaagg aacacucuaa cauuauggga auccagcaac uagagaccau caaagucaca     3060

gauaagaaug gcaacgagaa aagaguguuu aaggcaccua ugccaaauuu ccgacagguu     3120

acuaaagcgu uucuggaaaa cauucuuauc ucccacaagg ccaagaauaa aguggucacc     3180

aagaacaaaa auaagguggc ugguaguaac aaaguacagg aagugcucac gccccgcggg     3240

cagcugcaua aggagaccgu uuacggcaag uaccaauauu acguccagaa agaggaaaag     3300

auuggaggca aauucgacuu ggagacaaua aagaaagugg ccaauccgcu guaucagagc     3360

uuacucaaga agcggcugga agagaacggg ggagacccua aaaaggcauu uacugguaaa     3420

aauucucuug cuaagaaccc caucuaccug gaugacgccc aaaccgaaca guugccagag     3480

aaagugaagc ucgucuggcu ggaaaauucc uucucaauua gaaaagauau cacaccugaa     3540

aacuuuaagg acgagaagcu aauugauaaa auccuggacg aaggcguuaa gaggauacuu     3600

aaagagcguc uccagaguuu cgggaaugau gcaaagaaag ccuucagcga ccuggagaag     3660

aaccccaucu gguugaacaa ggaaaaagga auuacuauca agcgcgugac cauuucgggc     3720

guggcuaaug cggagccauu acacacgaaa aaggaucauc uggguaaaga aauccucgac     3780

aaggagggga agcccauacc uguagauuuu auuuccacag gcucuaacca ccacgucgcc     3840

guguaucggg acgaaaaagg aaaucugcag gagaagguug ugagcgucuu cgaggcagug     3900

guuagagcua accaaggccu uccaaucauc gacaaaaccu acaaucagca ucuggggugg     3960

gaauuuuugu ucacuaugaa gcagaacgag auguuugugu ucccgaauga aucaacagga     4020

uuuaacccca augauauuga ccuacuggau ccuaaaaaca agaggauaau caguccaaau     4080

cucuuccgag uacagaagcu ggccaccggc gacuauuggu uucggcacca ucuugagacu     4140

accgucgaaa caaaauccgc cacgaaggau augaccuaca aacgcuuauc ucugaaccac     4200

auuaagaaua ucauuaaagu gagaaucagc caucucggug acauaguguc cgucggggag     4260

uacuga                                                                4266


<210>  3330
<211>  4275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3330
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa cagaugagga agucuccucu auaaucaggu uggguguucg ggugaacccc      120

uuaacuguag acgagacgca gaauuucgaa aaaggcaagu caauuaccac aaacgcagag      180

cgcacucuga aacgaaguau gcguagaaau cuacaaaggu acaagcuccg gcgcgaugcc      240

cugguggagu uucuuaaaga acugggguuc aucaccgacg agacaauuuu gagcgaacag      300

ggaaacagaa ccacuuuuga gacaugcagg cuccgggcga aggcugccga ggaagagauc      360

ucccuggagg aacugucucg cguccucuua augauaaaua agaaacgagg cuauaagucg      420

ucaagaaaag ugaagaaaga agaggauggu guucugaucg acgggaugga gauugcaaag      480

aaguuauacg aagaggauuu gaccccugga cagcugugua gccagcuacu cgaaaguggc      540

aaaaaggugc ugccagacuu cuauaggucc gaucuucaag aggaacugga ccggaucugg      600

ucuguccaga aacaguuuca cccggaugug uucugcgacg ccgcuaagga ggagauuaaa      660

ggcaagaacc guagccagac gugggcaauc uuggccaauu acuuuguaug gaaagaagag      720

guuguggggu ggaacgaucg cgaagcuaag aaugagacua uagaaaagga guguaaacuc      780

gucggacuga agagaaccac aaaagguuau gaguuaaaga aagaaaacua cagguggcgg      840

gcccaagcgc ugacugagca gcucggccuu gaagagauug caguggugcu gcaggagauc      900

aaugggcaga uuaacgccuc cucaggauau uugggcgcua ucagugaccg aagcaagguc      960

cuauacuuca aucaucaaac cguuggucag uaccagaugg ccgaacugga caagaacccc     1020

cacgcaucuc uccgcaacau gguguuuuau agacaagauu accuggacga guucgauagg     1080

aucugggaaa aacaggcuga guuucauaag gaacuuacag ccgagcugaa aaaggagauu     1140

cgggacauga uaaucuucua ucagcgcaga uugaaauccc agaaggggcu cauuucauuu     1200

ugcgaauuug agaggcugga aaucguagug gagaaggaug gaaagaagca aaccaaaguc     1260

auaggcugua aggugauucc ucguagccac ccauuauucc aggaauuuaa aguuuggcag     1320

acgcugaaug acaucaaggu gcuaggccgg gagaagaaac gaaacgcgcu cagugccucu     1380

gaguccagag cacuguaccc cgaagagaag gaaauccuug cuagggaguu ggccauuaaa     1440

gaaaauauga agaaagcuga uguccugaag cuccuguucg agaacccuca ggagcuugac     1500

cugaauuuua aacaaaucga ugggaaccgc acuggauuca gcuuguuuuc ggccuauucu     1560

aagaugauug aaaaguacgg uuaugagcca cucgacuuca aaaagcccgc agaugaaaua     1620

aucggccagc ugcggacaau uuuuaccgac uuagggugga auacuaaccu guucacaauc     1680

gaccuaacca aagagggaaa ggagcucgaa augcagccuu acuuuagacu guggcaucuu     1740

uuguacuccu ucgagggcga uaauacguca accgguaacg ggaaacugau cgaaaagauu     1800

augcagcucu gcggcgugga gaaggaauau gccguagagc uggcuagucu uagcuuucaa     1860

gacgauuacg gaucccuguc ugcgaaagca auaaagagga uccuuccaua uuugaaagag     1920

ggcaaucucu acgacguugc cugugaauau gcuggguacc gccacagcaa gucaagucug     1980

acaaaagagg aaauugagaa caaggugcua aagggaaaac uggaaguccu cccgaaaaau     2040

ucccugcgaa accccguggu cgagaaaauc cuuagccaga uggugaaugu uauuaacacu     2100

aucauagaua ccuaugguaa gccugacgag auccgggugg aauuggcccg ugagcugaaa     2160

aagucugcaa aggaaagaga ggaauuaaca aaagccauug cuaagucaac uagggagcac     2220

gaggaaaucc gcaaacuccu gcaggaugag uucggcauga ugaacguaag ucggaaugac     2280

auuaucagau acaaacuuua cgaggaacug aaggauaacg gguauaagac cuuguacagc     2340

aaucaguaua uaccaaaaga gaagauuuuc uccaaggaaa ucgacauuga gcauaucaua     2400

ccccaaucuc gacucuucga ugacucgcug agcaacaaaa cacuagaaua caaggccauc     2460

aauauugaga aaggaaacaa gaccgcauau gauuucguca aagaaaagua cggcgaggag     2520

ggucugcagc aguauaucaa uaggugcgaa ucccucuuua acgacaagaa agcgaagcug     2580

cgcaaacuua agauggagca aaaagacauu ccugaugggu ucaucgaccg ggauuugaga     2640

aauacucagu acauagcuaa gaaggcccug gcaauguuaa acgaaauuug uaggcgugug     2700

guugccacga caggcucaau caccgaccag cuccgcgagg auuggcagcu gguggacguc     2760

augaaagaac uuaaucugcc aaaguacgag gcucuaggau uuguggagac uuaugaagau     2820

aaagacggcc ggaagaucaa aagaauuaag gauuggacca agaggaacga ccaccgacau     2880

cacgccaugg augcacucac aguagcuuuc accaaagacg ccuuuaucca auacuucaau     2940

aacaagaaug cggcccagga uagugggaac cggaaccaca cuaauauuau aggaaucaaa     3000

acacgcuauu uugacaaggg uagagcucug cccccgauuc cuuuggagca guucaggauc     3060

gaagcaaaac agcaucugga gaaguuacuc gugucuauua aggccaaaaa caagguuguc     3120

acggugaaug ugaaccggac caaaaagcga aaaggcgaaa auacuaagau ccaacagaca     3180

cccagagggc agcugcaccu ugagaccguc uacggaagcc auaaacagua uguuacaaag     3240

auagagaagg ugaacgcuuc cuuugacgcc gcaaaaaucg cgacuguguc aaagcgcgcc     3300

uacaggaaug cucuguugaa acgucucgaa accuucggca acgauccaaa gaaagccuuu     3360

acggguaaga augcacugga gaagaacccu cuauaugcug acaaauacca aaccauucgg     3420

guacccgaaa agguccagac aguggaguuc gagacuaucu acaccauuag aaaaccaguu     3480

gaucccgccc ugaaugugga caaggucgug gauguuaaag ugagggcaau ccuugaacgc     3540

cgacucaagg aguauggggg cgacccuaag aaagccuuug uaaacuucug ggaaaaucca     3600

auauggcuga acaaggagaa aggaauuagc aucaagagag ucucuauccg gggcauuaau     3660

aacgcucagu ccauacacgu gaaaaaggau aagaacggga auccgaucug ggacgaaaac     3720

ggaaaacaaa uucccgugga uuucgucaau acaggcaaca aucaucacgu ugcgaucuac     3780

aggaagccug uguuggacaa aaagggucag guaacuuuug augaggacgg gaaccugaug     3840

uaugaguuag acgaaguggu cgugccauuc uuugaggccg uuacccgcgc aaaucucgga     3900

cugcccauua ucgauaaaga cuaccguaag agugaaggcu ggcaguuccu uuuuagcaug     3960

aaacagaacg aguauuucgu guuuccuaau gaaaagacgg guuucaaccc aaaggagguc     4020

gaucuguuga aucccgacaa cuacgcuaug auaucuccga aucucuuuag agugcaaaca     4080

auguccaaag uuauguaugg caacaaugug guccgggauu acaaguucag gcaccaucug     4140

gagaccacug uaaaagacau gaaggaacua aaagauaucg ccuacaagca guauaagaca     4200

cugucauuug ggaacagugu gguuaaaauu cgcaucaauc acauuggaca gaucgugagc     4260

gucggcgagu acuga                                                      4275


<210>  3331
<211>  4275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3331
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa cagaugagga agucuccucu auaaucaggu uggguguucg ggugaacccc      120

uuaacuguag acgagacgca gaauuucgaa aaaggcaagu caauuaccac aaacgcagag      180

cgcacucuga aacgaaguau gcguagaaau cuacaaaggu acaagcuccg gcgcgaugcc      240

cugguggagu uucuuaaaga acugggguuc aucaccgacg agacaauuuu gagcgaacag      300

ggaaacagaa ccacuuuuga gacaugcagg cuccgggcga aggcugccga ggaagagauc      360

ucccuggagg aacugucucg cguccucuua augauaaaua agaaacgagg cuauaagucg      420

ucaagaaaag ugaagaaaga agaggauggu guucugaucg acgggaugga gauugcaaag      480

aaguuauacg aagaggauuu gaccccugga cagcugugua gccagcuacu cgaaaguggc      540

aaaaaggugc ugccagacuu cuaucacucc gaucuucaag aggaacugga caggaucugg      600

ucuguccaga aacaguuuca uccggaugug uucugcgacg ccgcuaagga ggagauuaaa      660

ggcaagaacc ggagccagac gugggcaauc uuggccaauu acuuuguaug gaaagaagag      720

guuguggggu ggaacgaucg ugaagcuaag aaugagacua uagaaaagga guguaaacuc      780

gucggacuga agcgcaccac aaaagguuau gaguuaaaga aagaaaacua cagauggagg      840

gcccaagcgc ugacugagca gcucggccuu gaagagauug caguggugcu gcaggagauc      900

aaugggcaga uuaacgccuc cucaggauau uugggcgcua ucagugaccg gagcaagguc      960

cuauacuuca aucaccaaac cguuggucag uaccagaugg ccgaacugga caagaacccc     1020

caugcaucuc uccgaaacau gguguuuuau cgccaagauu accuggacga guucgauaga     1080

aucugggaaa aacaggcuga guuucacaag gaacuuacag ccgagcugaa aaaggagauu     1140

agggacauga uaaucuucua ucagcggcgc uugaaauccc agaaggggcu cauuucauuu     1200

ugcgaauuug agagacugga aaucguagug gagaaggaug gaaagaagca aaccaaaguc     1260

auaggcugua aggugauucc uaggagccau ccauuauucc aggaauuuaa aguuuggcag     1320

acgcugaaug acaucaaggu gcuaggccgu gagaagaaac ggaacgcgcu cagugccucu     1380

gagucccgag cacuguaccc cgaagagaag gaaauccuug cuagagaguu ggccauuaaa     1440

gaaaauauga agaaagcuga uguccugaag cuccuguucg agaacccuca ggagcuugac     1500

cugaauuuua aacaaaucga ugggaacagg acuggauuca gcuuguuuuc ggccuauucu     1560

aagaugauug aaaaguacgg uuaugagcca cucgacuuca aaaagcccgc agaugaaaua     1620

aucggccagc ugcgcacaau uuuuaccgac uuagggugga auacuaaccu guucacaauc     1680

gaccuaacca aagagggaaa ggagcucgaa augcagccuu acuuucggcu guggcaccuu     1740

uuguacuccu ucgagggcga uaauacguca accgguaacg ggaaacugau cgaaaagauu     1800

augcagcucu gcggcgugga gaaggaauau gccguagagc uggcuagucu uagcuuucaa     1860

gacgauuacg gaucccuguc ugcgaaagca auaaagagaa uccuuccaua uuugaaagag     1920

ggcaaucucu acgacguugc cugugaauau gcuggguaca ggcacagcaa gucaagucug     1980

acaaaagagg aaauugagaa caaggugcua aagggaaaac uggaaguccu cccgaaaaau     2040

ucccugcgca accccguggu cgagaaaauc cuuagccaga uggugaaugu uauuaacacu     2100

aucauagaua ccuaugguaa gccugacgag auccgagugg aauuggcccg ggagcugaaa     2160

aagucugcaa aggaacguga ggaauuaaca aaagccauug cuaagucaac uagagagcau     2220

gaggaaauca ggaaacuccu gcaggaugag uucggcauga ugaacguaag ucgcaaugac     2280

auuauccggu acaaacuuua cgaggaacug aaggauaacg gguauaagac cuuguacagc     2340

aaucaguaua uaccaaaaga gaagauuuuc uccaaggaaa ucgacauuga gcacaucaua     2400

ccccaaucua gacucuucga ugacucgcug agcaacaaaa cacuagaaua caaggccauc     2460

aauauugaga aaggaaacaa gaccgcauau gauuucguca aagaaaagua cggcgaggag     2520

ggucugcagc aguauaucaa uaggugcgaa ucccucuuua acgacaagaa agcgaagcug     2580

cgaaaacuua agauggagca aaaagacauu ccugaugggu ucaucgaccg cgauuugcgg     2640

aauacucagu acauagcuaa gaaggcccug gcaauguuaa acgaaauuug uagaagggug     2700

guugccacga caggcucaau caccgaccag cuccgugagg auuggcagcu gguggacguc     2760

augaaagaac uuaaucugcc aaaguacgag gcucuaggau uuguggagac uuaugaagau     2820

aaagacggcc gcaagaucaa acggauuaag gauuggacca agagaaacga ccauaggcac     2880

cacgccaugg augcacucac aguagcuuuc accaaagacg ccuuuaucca auacuucaau     2940

aacaagaaug cggcccagga uagugggaac cgaaaccaua cuaauauuau aggaaucaaa     3000

acacgguauu uugacaaggg ucgcgcucug cccccgauuc cuuuggagca guucagaauc     3060

gaagcaaaac agcaccugga gaaguuacuc gugucuauua aggccaaaaa caagguuguc     3120

acggugaaug ugaacaggac caaaaagcgg aaaggcgaaa auacuaagau ccaacagaca     3180

ccccgagggc agcugcaucu ugagaccguc uacggaagcc acaaacagua uguuacaaag     3240

auagagaagg ugaacgcuuc cuuugacgcc gcaaaaaucg cgacuguguc aaagagagcc     3300

uaccgcaaug cucuguugaa aaggcucgaa accuucggca acgauccaaa gaaagccuuu     3360

acggguaaga augcacugga gaagaacccu cuauaugcug acaaauacca aaccauucgu     3420

guacccgaaa agguccagac aguggaguuc gagacuaucu acaccauucg gaaaccaguu     3480

gaucccgccc ugaaugugga caaggucgug gauguuaaag ugagagcaau ccuugaaagg     3540

cgccucaagg aguauggggg cgacccuaag aaagccuuug uaaacuucug ggaaaaucca     3600

auauggcuga acaaggagaa aggaauuagc aucaagcgag ucucuaucag aggcauuaau     3660

aacgcucagu ccauacaugu gaaaaaggau aagaacggga auccgaucug ggacgaaaac     3720

ggaaaacaaa uucccgugga uuucgucaau acaggcaaca aucaccacgu ugcgaucuac     3780

cggaagccug uguuggacaa aaagggucag guaacuuuug augaggacgg gaaccugaug     3840

uaugaguuag acgaaguggu cgugccauuc uuugaggccg uuaccagggc aaaucucgga     3900

cugcccauua ucgauaaaga cuaccgcaag agugaaggcu ggcaguuccu uuuuagcaug     3960

aaacagaacg aguauuucgu guuuccuaau gaaaagacgg guuucaaccc aaaggagguc     4020

gaucuguuga aucccgacaa cuacgcuaug auaucuccga aucucuuucg ugugcaaaca     4080

auguccaaag uuauguaugg caacaaugug gucagagauu acaaguuccg gcaucaccug     4140

gagaccacug uaaaagacau gaaggaacua aaagauaucg ccuacaagca guauaagaca     4200

cugucauuug ggaacagugu gguuaaaauu aggaucaauc auauuggaca gaucgugagc     4260

gucggcgagu acuga                                                      4275


<210>  3332
<211>  4278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3332
augagaagga acauccuggg ccucgaccuu gggaccacaa gcauuggauu cgccaaggug       60

auagaggaug aagacuacca aaauuccgcu gucgagcuga ucgguguucg ggugaacccc      120

uugacuacgg augaacagac caauuuugag aaaggccgcc cuguaucugu gaacgcagac      180

cgaacauuaa agcgugggau gagaaggaau cuggaucggu aucagcuacg cagacaccau      240

cucauugaag uccugaaagc caaccacauc auuacugugg acaccaaacu ugcggaggau      300

ggaaagaaua caacccaugc uacuuacgcc guuagggcac uggccgcuac agcagagauc      360

ccauuggaag ccuucgcucg ggugcuccug gccauaaaca agaagcgcgg cuauaaauca      420

agucgaaagg cgaaaaauga ggacgaaggu cagcugaucg augggauggc agucgccaag      480

agacucuacg aggagaacuu aaccccggga cagcugagcu aucuuuugcu gaaagaaggc      540

aagaaguacc uacccgacuu uuauaggucc gaucucaaug cugagcugga caaagugugg      600

ucuuuccaaa agcaguuuua cccugaaauu cuuacggagg aguucaaaaa gcaccuggaa      660

ggcaaagggc agcgggccac uucggcagcu uuuuggggac aguacgguuu caacaccgcc      720

gagaauaagg gcucacguga agagaaaaag uugaaggcgu aucaauggcg cagcgaagcc      780

guagaucagc agcucacaaa agaggaaguu gcauacguga ucacugagau uaacaaugac      840

cugaacaaga guuccgggua uuuaggagcu aucucugaca gaagcaaaga gcuguuuuuc      900

aacaagcaga ccgucggcca auaccucuau cagcagcuua aacaaaaucc acauacaagg      960

cugaaggguc agguguuuua ccggcaggau uauuuggacg aguucgaaac cauaugggaa     1020

acgcaggcca aguuucaccc cgagcuaacu gcugcccuga aagaagagau ucgagaugug     1080

aucauuuucu accaacgcaa gcucaaaucc cagaaggggc ugaucucauu cugcgaguuu     1140

gaaaguaaag agaucgaagu cgagaagaac ggacuuaaga uuaaaaagag aauaggccug     1200

aggguugcac cuaaaagcuc uccauuguuc caggaguuua agaucuggca agugcucaau     1260

aaccuggagu uccggaauaa auccucaaag gaaguauuug ugcccgacau gacagccaag     1320

cagcaguuau ucgaugaacu gaacauuaaa ggcaaucuac ucgcuagcga ggcgaucaag     1380

auacuggggu acaaaaccaa ggacugggaa gcaaacuauc acacucuuga gggaaaucgc     1440

acaaacaaaa guuuguacga ugccuaucug aagauucucg agcuggaagg uuacgacgag     1500

gaucuucuga aauugucugg cuccgacgau aucgacguca gcaagaucga aaagucggcu     1560

ucugagauua aacagauggu gcaugccguu uuugaagugc ucgggaucaa uuccaccauu     1620

cuggacuucg augcagaggc cgacggaaag gcuuuugagc agcaaacggc cuauagauua     1680

uggcaucugc uauacucagc agaagaugac aacaaaaagu aucuccagga ggauauauuc     1740

aucuacggcc acgacaauau uggucugaaa aagaaacuuu gugaaagguu uggguuccgu     1800

ccugagcaca ccaaaaucuu ggucaacgug gcguuuagug gcgauuacgg aagccugucc     1860

ucuaaggcua ugcggaaaau cuauccauuc auuaaggaac ucacauacga caaagccugc     1920

cgagaggcag gcuauagaca cagcgccuca agucugucca aggaggaaau agcuacuagg     1980

ccguuacaga agcgccuggg gcuuuugaaa aagaaugagc uccggagccc cguaguugaa     2040

aagauccuga accagauggu gaacgucgug aaugcccuaa uugaugacga gaacaagaga     2100

aggaaagcac gcggacuguc ugaugacuuc cauuuugaug agauccgaau ugaacucgcu     2160

cgggagcuga agaagaaugc caaagaacgu gcggagauga ccacaaacau caaugccgcu     2220

aagcaacuuc acgagaaaau aaucaagauu uugcagacuc aguucggugu cucaaacccu     2280

aguagaaaug acaucauuag guacaaacug uaugaagagu uaaagaacaa uggcuacaaa     2340

gaccucuaua ccaacacaua caucagccca gauauacugu uuuccaagca aauugacauc     2400

gaucauauua ucccccagau gucucuuuuc gacgauucgu uuagcaauaa gaccgugguu     2460

uuccgcaaag acaaccagga uaaggggaau aaaacugcau acgacuauau acugggaaag     2520

uacggcgaau uggcccucga ggaauauauc ucccggauua cgucacuguu ugagcuaggu     2580

aaaaagaaca aggaagaggg gaucaauaaa gcuaaguucc agaaacugca aaagagacuc     2640

auugauaucg gcgacggauu uauagagaaa gaucugaggg aaagucagua cauugccaag     2700

aaggcgaaaa acaugcuuua ugcaaucugu cgaucugugg uagccacaag cggcgagauc     2760

accgcucgcu ugcgggaaga cugggaucug gucaacguga ugcaggagau uaauuuaccu     2820

aaguucagag cccucgggcu gacugaaaug guugagaaaa aggacggagg ucagaaagag     2880

aggaucauug acuggaccaa gcguaacgau caccgccauc acgcaaugga cgcucuuaca     2940

auagccuuua cccggcacuc ccauauccaa uaccugaauc accuaaacgc aagaaaggau     3000

gaagccucaa aauggcaucc aguggucagc aagauugagg aaaaagagac ugaccucgug     3060

uaugaugacc ugggcaauag gaagcgacgg uucaaagcuc ccaugccgaa cuuucgcgag     3120

guagugaagg aacacuugga gaauguucug aucucucaca aggcgaaaaa caaggucgug     3180

acgaaaaaua agaacaaaau uaagacagcc aaaggggaaa agaucaagac cgaguuaacu     3240

ccuagaggac agcuccauaa agaaacaaua uacggcaggu accgguauua cgaguccaag     3300

gaggaaaaag ugggucccaa guucgaugca gagaccaucg ggauggucgu uaauccacag     3360

uaucgaaaac ugcuucugga aagauugaca acuaacggca augacccuaa gaaggcuuuc     3420

ggaggcaaaa acacccucag uaagaauccc cuguacauug augaggccaa acaggaacua     3480

cugccagaga agauccuucu ccgcuggcug aacgacgauu auagcauuag gaaagacgug     3540

acgcccgaga acuugaagga cgugaagacc aucgaaaaag uacuggauga ggggauaaag     3600

aaaauuuuac ucaagcgucu ggucgacuuu ggaaaugauc cuaaaaaggc cuucucugac     3660

cuugauaaga acccaaucug gcugaaugaa gcuaaaggca ucacaauuaa gcgggugacu     3720

auauccggug uuaaaaacgc agaggccuug cacuacaaga aagaccauuu ugggaaugcg     3780

auccucaacg aaaauggaaa gcugauuccg ucaaguuucg ugagcaccgg caacaaucac     3840

caugucgcua ucuauagaga ugccgagggu aaccuacaag agaggguggu uucgcuguuu     3900

gacgcagugc agcuuguaaa ugccggcgaa gcugucauug aucgcgagua caacaaaggg     3960

aucggauggc aguuccucuu uacaaugaag caaaaugaaa uguucguguu ucccaacgag     4020

aagacuggcu ucgacccuaa agagauagau cuguugaaua ccgacaaccg aaaguuaauc     4080

uccccaaauc uguuuagagu gcagaaaauu gcaucuaaga acuacuucuu ccggcaccac     4140

cucgaaacga acgucgagga aaggaaagag cugaaggaua ucguuuauaa gccccagcuu     4200

gggcugaaug ccauuaaaga cgugguaaag gugcgcauca accauuuggg aaaaauaauu     4260

agcgucggcg aauacuga                                                   4278


<210>  3333
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3333
augcccagaa agaacauccu gggccucgac cuugggacca caagcauugg auucgccaaa       60

auaaucgagg augacgauua ccagugcucc ucuguggaac ugauuggugu caggguuaau      120

ccuuugacua cggacgagca aaccaacuuu gaaaagggca aaccaguguc aguaaaugcu      180

gaucggacau uaaagcgcgg gaugcgacgu aaccuggaca gauaucagcu aaggcgggau      240

uaccucaucg agauucugcg cacucaccau aucauaaccg cagacacaaa acuugccgaa      300

gauggaaaga auaccacuca cacaaccuau gcgcugagag cuuuggccgc aacggccgag      360

aucccgcucg aggcuuucgu gaggguccug cuggcaauua acaagaaacg gggcuacaag      420

aguagccgca aagccaagaa ugaagacgag ggucagcuca ucgaugggau ggcuauugcc      480

cgaagauuau augaagaguc ccugacuccc ggacaguuuu cuuaccaacu uuugaaagag      540

ggcaagaagu aucugccuga cuucuacagg ucggaucuaa acaccgaacu caauaaagug      600

uggaacuuuc agaagcaguu cuacccagag auccugacag acgaauuuaa acggcaucuu      660

gagggcaagg ggcagcgugc gacugcaguu gccuucuggg gacaacacgg uuuuucaaug      720

gcugaaaaua aaggcagccg cgaggagaag aaacugcaug ccuauaagug gagaagugaa      780

gcagcucaca agcaguugac caaagaggaa guggccuucg ucauaacaga gauuaacaau      840

gaccucaaca aguccucugg guaccuggga gcgaucagcg auagguccaa agaauuauuu      900

uucaauaagc agaccguggg ccaguaucug caccaacagc ucaaagagaa cccccauacg      960

cggcuuaagg gucagguauu uuaccgacaa gacuaucugg augaguucga aacuauuugg     1020

gagaugcagg ccaaguucua cucagcauug acacuagaac ugaaagagga aguucgcgac     1080

guggucaucu uuuaucagag aaagcucaaa agucagaugg ggcugaucag ccuuugugag     1140

uucgagucua aggaaauuga gauagagaaa aacggagaaa aggcuaagaa aaggaucggc     1200

cugcgggugg ccccuaaguc cucaccauug uuucaagagu ucaaaauuug gcaagugcuc     1260

aauaaccugg aauuucgcaa uaagaaaacc aaggaggucu ucguucccga aauggcugcc     1320

aagcagcagu uauuugauga gcugaacauc aaaggcaauc uacucgcaag cgaagccaua     1380

aagauucugg gguacaaaac uaaggacugg gaggcuaacu accacacacu ugagggaaau     1440

agaaccaaca aaaguuugua ugaugcgugg cugaagaucc ucgaacugga ggguuacgac     1500

gaaaaucuuc ugaaauuguc uggcuccgau gacaucgaug ugagcaagau ugagaagccu     1560

gcacucgaaa ucaggcaaau gguacuggcc guguucgagg ucuuagggau uucgggagcu     1620

auacuggacu uugacgccga ggcagauggc aaagccuucg aacagcagcc agcuuaucag     1680

cuauggcauc uccuguacuc ugccgaggac gaugguaaga aauauuccca agaagacauc     1740

uuuacguacg ggcacgauaa cauuggccuu aagaaaaagu ugugcgagaa guucggauuu     1800

cguccggaac acaccaaaau ccuggugaau guuguguucu cagacgauua uggcaaccuc     1860

aguagcaagg caaugcggcg aaucuacccc uucauuaaag agcugacaua cgacaaggcg     1920

ugugcugagg ccggguauag acauuccgca gccucuuuaa cuaaagaaga gauagcugcc     1980

aggccucugc agaaucgccu ugauuugcuc aagaagaacg aacugcggag cccagucgug     2040

gagaaaaucc uaaaucagau gguaaacguu gugaacaccc uggucgacga ugagaaucag     2100

aacagaaagg caaggggacu cucagaagac uuucacuucg augagauucg caucgaacug     2160

gcucgagagc uuaaaaagaa ugccaaagaa cgggcggaga ugacaacuaa cauuaauacc     2220

gccaagcaaa gucaugagaa gaucauacgu aucuugcaga cagaauuugg ugugcccaac     2280

ccuuccagaa augacauugu cagguacaaa cuguaugagg aauuaaagaa caauggcuac     2340

aaagaccucu auaccaacac uuacaucagc cgcgauauuc uguucucuaa gcagaucgac     2400

guggaucaca uaauuccaca aaugucacuu uuugacgaua guuucagcaa uaaaacgguu     2460

guguuucgga aggacaacca ggauaaaggg aauaagacag cuuaugacua cauccuggga     2520

aaguacggcg agcaggcauu ggaagaguau auuuccagaa ucaccucucu cuucgagcug     2580

gguaaaaaga acaaagaaga ggggauaggc aaggccaaau uucagaagcu acaaaagagg     2640

cugguagaua ucggagacgg cuucauugaa cgagaucucc gcgagucgca guacaucgcu     2700

aaaaaggcca auaacaugcu guaugcaauu ugccggagcg ucguguccac uucaggggaa     2760

aucaccgcga gacuuaggga ggacugggau uugguuaacg ugaugcagga gauaaaucug     2820

cccaaauuuc gugccuuagg acucacagaa auggucgaga agaaagacgg ugcucagaag     2880

gaacgcauua ccgacuggac uaagcggaac gaucauagac accacgccau ggacgcacug     2940

gcuaucgccu ucacaaggca uagucacauc caauaccuua aucaucugaa cgcacgaaaa     3000

gaugaggcca auaaguggca cccgguggua ucuaaaauug aggaaaagga gacggugcua     3060

aucuaugacg aucucggcaa ccggaaacgc aaguuuaagg cuccuaugcc caauuucaga     3120

gaaguuguca aagagcaccu ggaaaacgug uugauuagcc auaaggcgaa aaauaaggug     3180

gucaccaaaa acaagaauaa gauaaaaacu guuaggggag agaaggugaa aacagagcug     3240

accccacggg gacaguuaca caaggaaaca aucuacggcc gauauagaua cuacgagucc     3300

aaagaagaga agauuggucc uaaauucgac gccgaaauga ucgggauggu gacuaacccc     3360

caguauaaga agcuccugcu ugagcgccug gcagcuaaug gcaacgaucc caaaaaggcc     3420

uuuuggggga aaaaugccuu gaacaagaac ccacucuacg uagacgaggc uaggcaggaa     3480

cugcuaccug agaaaauucu gcuucguugg cucaaugaug acuauucaau ccggaaggau     3540

gucaccccag aaaaccugaa ggacauaaaa acgaucgaga agauuuugga caaaggcgug     3600

aagaaaaucc uguuaaagag acucgcagau uucgggaaug acccgaagaa agccuuuagc     3660

gaucuggaca agaaccccau uuggcaaaau gaagcgaaaa ggaucgcuau aaagcgcguu     3720

accauuucug gaaucaaaaa cgccgcaccu cuucaucaca agaaggauca ucugggcaau     3780

gacaucuugg augagaaagg uaagcagauu ccauccaguu ucgugagcac agggaacaau     3840

caccacgucg ccauauaccg agacgcugag ggaaaccucc aggaaagagu gguuucucug     3900

uuugaugcag ugcagcuagu aaauggcggu gagcccguca ucgacaaaga auauaacaag     3960

ggcauugggu ggcaguuccu guuuacuaug aaacagaaug agauguucgu guuuccugau     4020

gagaagaccg gauucaaccc acgggaagug gaccuucucu ccucaggcaa uaggaaacug     4080

aucaguccca acuuguuucg cguccagaag uuagccagca agaauuacuu cuuccgucau     4140

caccuggaga caucgguuga agagaaaaag gaacucaaaa acauugcgua uaagccgcaa     4200

cuggggcuua acgcucuguc caauaucgug aaaguaagaa uaaaccauau cggagagauu     4260

gugucugucg gcgaguacug a                                               4281


<210>  3334
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3334
augaagaaca uccugggccu cgaccuuggg auuaccagca uauccuucgc ccacaucgug       60

gaggaugaaa auaaagagaa gucugaaauu aaagagcugg gagucagaau cguuucauug      120

acaacugacg aacagaguga uuuugagaag gguaaaagca uuacgaccaa cgcuaauagg      180

acauuaaagc auggcgcacg gcugaaccua gaccgcuacc aacagcgacg uaaguaucuc      240

aucgaucugc uucagaaagc caaucugaua acuuccucuu cgaucuuggc ggagaacggg      300

aagaauacca cacacucaac cuggcagcuc agagcuaaag ccgugacuga aaggauugag      360

aaggaagagu ucgcacgggu acugcuggcc aucaacaaaa agcgcggaua caagagcagu      420

agaaaagcua agacagagga cgaaggccaa gcaauugaug guauggccau cgcuaaaagg      480

cucuaugacg agaauuuaac ccccgggcag cugucccuuc aguugcugca gcaaaacaag      540

aaacuacucc cugauuuuua ccggucugac cugcagcgcg aguucgaucu uguguggaau      600

uuucagaagc aguucuaucc agacauacug acggauagcu uuuacaaaga guugcaagga      660

aagggcaagg acgccacuuc caaagcguuc ucaaagcgau aucauuuuga caccgcagaa      720

aacaaaggca guaaggagag cgucagacuc caggccuacc aguggagggc ugaggccauu      780

ucuaaacaac uguccaagga agagguggca uacguuuuaa cagaaaucaa uaacaaucug      840

aacaacgcuu caggguaucu cggagccauu agcgaucgga guaaggagcu uuacuucaau      900

cgucagacug ugggucagua ucuguacgcg caguugcaag aaaaccgcca caauucucug      960

aaaaacaagg ucuuuuauag acaggacuac cuggaugagu ucgagaggau cugggaaacc     1020

caggccuccu uucauaaaga gcucacagac gaacugaaga aacagauccg ggauguggua     1080

auuuucuauc aacgaaaacu uaagagccag aaaggccuga uaucguucug cgaguuugaa     1140

ucuaaggaga ucgagauuga aaaagacggg aagaccauca cgaaaacuau aggagcacgc     1200

guugugccga aguccucacc cuuguuccag gaguuuaaga uuuggcagau ccucaauaac     1260

gucacaugua aaagaaaggg caucaggaaa aagaaaagua gcgcuaaguc caccgaacug     1320

gauuuauuca augagccuuc ucaaacuauu uuuagccugg acauggagug caaacagcua     1380

cucuucgaug aacugaaccu uaagggugac uugaagucag auaaagugcu gaagcuccug     1440

ggguacaguc cacaggagug ggaaaucaau uacaaccagc uugagggaaa ucggacacaa     1500

aaagcccugu augaagcaua cuugaagauu guggagaugg aggcccacga cgucaaagac     1560

auacuccaga ucaaguccgc aaaggaugac uggagccugg augaaucucc cuuaucagcc     1620

agugagauua aagaaaaggu uaaagacauc uuucaggcuc ugggcaucug uaccaagauu     1680

cuauauuucg auccucuccu gccagugaaa gaguuugaaa agcaagacag cuaccagcuu     1740

uggcacuugc uguauuccua cgagucugau gacucgacga gcggcaacga gacccucuau     1800

cgcauacugg aaaagaaaua cgcguucaag agagagcaug caaggaucuu agccaaugua     1860

gcucugcagg augacuacgg gucccuuuca acaaaagcca uucguaagau cuaucccaac     1920

auuaaagaaa aucaguacag uaaggcaugc gaguuggccg gauauaagca cucuaaacuc     1980

agccugacua ccgaagaacu agaggcucgg gaacugaaga acaucauacc ucuccugaaa     2040

aagaacgccc uucgaaaucc aguggucgag aaaaucuuga accaaaugau uaaugugguu     2100

aacgcacuga ucgaaaagaa cuccgagaga gaugcggagg guaaaauuac aaaguacuuu     2160

cauuucgacg aaaucaggau agaguuagcu cgcgaacuca agaaaaaugc ccagaagcgg     2220

uaugagauga cucagaauau uaacaaagca aagcuggaac accagaaaau cucagagauu     2280

cuucaaaaga aguuuggcau caaaaauccg accaagagcg auauaaucag auacaggcug     2340

uaucaggagu uggaacacaa cggguacaaa gagcucuaca caaaugcccc cauugcucgc     2400

gacaugcugu ucucuaagaa caucgaaauu gagcauaucg ugccuaaagc ccgagucuuc     2460

gacgauuccu uuaguaauaa gacccuaacu uuccaccgga uaaacagcaa uaagggagaa     2520

uauacggcau uugacuacau uacaucucug aacuccgagg aggaacucaa ccaguaucug     2580

acccgugugg agaaugcuua caaaacuaag ucaaucaguc uuaccaaaua uaagaacuug     2640

cugaaaaagg ccagcgaaau cggcgauggu uucauuaaua gagacuuaag ggauacacag     2700

uacaucgcga agaaagccaa ggagauucuc uuucaaguaa ccaaaaacgu ucugucgacu     2760

uccgggucua uaacagaccg ccuucgggaa gauuggggcc ugguggacgu caugaaggag     2820

cuaaauaugc caaaauauca gagccucgga cugacggagg uggaagagag aaaggaaggc     2880

aacaagguca ccgugaucaa aaauuggacu aagaggaacg aucaucgaca ccaugcuaug     2940

gacgcauuga caguugccuu caccaaaccc ucauacauuc aguaccugaa ucacuuaaac     3000

gcucgcaagg augagaauaa caaaaauuau agugugaucc ucgccauuga ggaaaaggag     3060

acaaucaaag uaccuacuaa caaugggaag aacaagcggg ucuuuauaga accaaucccc     3120

aacuucagac aaguggcuaa aaagcaccug gaggaaauuu uuaucuccca uaaagcgaag     3180

aauaaaguug ugaccaagaa cgucaauaag ccggccggaa cggacaaaca gcagauuacc     3240

cuuacaccua ggggucagcu gcacaaggag acuaucuacg gcaaauauca auacuaugug     3300

aacaaggagg aaaaaguagg ggugaaguuc gaugagcgua ccauaacaaa gguuucuaau     3360

cccuuguacc gcgaagcucu ccugaaacgg cuacaggcca acgacaauga cccaaagaaa     3420

gcauuuacug gaaagaacgc ucugagcaaa aauccuauuu aucuugauga gaccaagacg     3480

aagacacucc ccgaaaaagu caaccugacc uacuuggagg aggacuucuc caucagaaag     3540

gauaucucac cagacaauuu uaaagaucua aagagcauug aaaaagugau agaccagggc     3600

gugaagagga ucuuaauuaa acgacuccag gauuacgaca acgaucccaa gaaggccuuc     3660

gucgaccugg agaaaaaucc uaucuggcuu aacaaggaaa aagguauugc aaucaagcgg     3720

guuacuauaa gugggguguc uaaugcccaa ccacugcaua ucggcaaaga ucacuuggga     3780

aagacaaccc ucaacaagga gggcaaugaa auuccggugg acuauguauc cacugggaac     3840

aaccaccaug ucgcuaucua ccgcgacaaa gagggaaauc ugcaggagca gauugugagc     3900

uucuuugaag cgguugugag aacccagcaa ggucuaccca ucauagauaa gacauauaaa     3960

caggaggaag gcuggcaguu ccuguuuacg augaagcaga acgagauguu cgucuuuccu     4020

aaugccacca cuggguucaa cccagcagag auugaccuuc ucgaucccaa aaauaagaag     4080

cugaucucgc cuaacuuguu uagggugcaa aagaucgcua caaaggacua cuucuuucgg     4140

caccauuuag aaaccaaugu ugagacagau aacgugcuga aaaauacuac cuggaagcga     4200

gaaggacucu caggccugaa agacauugua aaggucagaa ucaaccaccu uggugauaua     4260

gugucuauug gggaguauug a                                               4281


<210>  3335
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3335
augaagcaca uccugggccu cgaccuuggg accaacagca uuggaugggc ccugauaaaa       60

caggauuucg agaauaagca aggugaaauc uugggcaugg gguccagaau uauccccaug      120

ucucaggaca uuuuaggaga uuuuggcaaa gguaacucag ugagucagac agcugagagg      180

acuaaguacc ggagcguccg ccgacugcgu gaaagauucc uacucaggcg ggagcgccug      240

cauagaaucc uuaauguucu gggguuuuug ccugaacacu augcauccca gauagacuuc      300

acgaaaaggu uuggaaaguu caaggaggag accgaaccaa aacucgugua cucuaacgag      360

ggcuuuaucu ucaagaaauc guuugaagag augcugucag auuucaagac acaucaaccg      420

cagcugcucg aggacaauaa aaagauuccc uaugauugga cuaucuacua uuuacggaag      480

aaagcccuga gccgcaagau ugaaaaagag gaacuugcgu ggaucuugcu gaacuuuaau      540

cagaagcgag gcuacuauca gcuaagaggg gaggaagagg aggaaaaccc uaauaaacuc      600

auagaguucu acagucugaa gauuguagac gugcuugcug augaaccaca aaaaggaaag      660

uccgacaucu gguacucucu gauuuuggag aacgguugga ucuauaggcg gagcuccaag      720

acaccccucu uugauuggaa agaaaagaca cgugacuuca ucgucaccau ugaucugaau      780

gacgacggcu cagugaaaac ugauaaggag gggaacgaga aacgcaguuu cagagccccu      840

ggagaaaaug acuggacauu aguuaagaag aaaaccgagc aggaaauaga uagcucuagg      900

aagacggugg gcacuuacau cuaugagacc cugcucaaaa acccaaagca gaaaauuaag      960

gguaaacuug uccggacaau cgaacgaaaa uuuuacaaga cugagcugaa acagauauug     1020

gagaagcaaa aagaguucca ccccgaacua cgccaggacg aucuguauuc cgacugcgug     1080

agagaacucu acaagaauaa cgaggcacau cagcugaccc uuucaaagaa agauuuugua     1140

caccuguuca uggaggacau uaucuuuuau caaaggccuu uggggagcca gaagaguucu     1200

aucuccaacu guacacucga guuccggaaa uacaaggaug aaaauggaac cgaacacacg     1260

caguaccuga aagccauucc aaagagcaac ccguauuacc aggaguuucg caucuggcaa     1320

uggauguaua auuuaaacau uuacaaaaag gaagacgaua cuaauguuac aaaggacuuc     1380

cugaacacca aagcugacuu ugagucgcua uucgaguuuc ucaauaacag aaaggaagug     1440

gaucagaaag cacugcuuaa gcauuucaaa uugaaugaca agacucacag guggaacuau     1500

gucgaggaua agaaauaccc cugcaaugaa acaaagacca ugauacuguc ucgucucgac     1560

aaaguggaga acaucuccga ugacuuucug acgcgggaaa uggagcagaa gauuuggcau     1620

aucaucuauu cagugaauga uaaagucgag uacgaaaagg cccuuaagag uuucgcuggc     1680

aaaaacaauc uggaggacaa cagcuucuuu gaagccuuca agaaauuucc uccauucaag     1740

uccgaguacg gcgcguuuuc ugaaaaagca auuaagaagu ugcucccccu gaugcgaaug     1800

gggaaauucu ggaacuauaa uaacauaagc gagcagucaa agaguagaau ccaaaagauu     1860

aucaccggag aguacgauga aaauauuaag gacaaaguua gggagaaggc cauccacuua     1920

acaacugaaa ccgauuuuca gggucugcag cuauggcucg ugcaguauau aguauacggc     1980

cgccacuccg aggcugccau gcuggggaaa uggaacagcg cagacgaucu ugaggaguuc     2040

uugaaggaau uuaagcaaca uucucugcgg aauccuaucg uggaacaggu cauuacagag     2100

acucucagag ugguuaaaga caucuggcag aaguauggaa acggcgcuaa agacuucuuu     2160

agggaaauuc acaucgagcu gggucgcgag augaaguuac cagccgagga acgagaacgg     2220

cugacccguc agauaucaga gaaugaaaac acaaauuuca gaauuaaagc gcuuuuggcc     2280

gagaugaugg augacaacag uguggagaau gucaggcccu uuagcccgau gcaacaggaa     2340

auccucaaga uuuacgagga aggggugcug aacuccgaua ucgagauaga agacgauauc     2400

cuaaagauuu cuaaaaccgc acagccuucg agcuccgacc ugaagcgcua uaaacucugg     2460

cuggagcaaa aguacaaauc acccuacacu ggccagauca uuccacuuaa uaaguuguuc     2520

acgccugagu augaaaucga gcauauaauu ccccagaguc gguacuucga ugacucuuuu     2580

acaaacaaga ucaucuguga aagcgcugua aauaaacuga aggauaacua uauuggauua     2640

gaguucauca aaaagcucgg cggguccauu guugagugcg gaaacgguaa aucaguggcc     2700

auacugaagc acgacgcuua cgaagauuuu aucaagaaac auuaugccaa uaacagaagc     2760

aagaggaaua aacuucuguu ggaggacauu ccagaaaaga ugaucgagcg acagaugaac     2820

gauacccgcu acauuucuaa auauaucucc ggcguccuca guaauauagu gcgggucgaa     2880

gacgggacug acgagggagu gaacagcaag aauaucguuc ccggcaacgg uaagauuacc     2940

acacaacuga aacaggauug ggggcuaaau gacgugugga acgaucugau ccucccuaga     3000

uucgagagga ugaaucaccu gaccaacucu acugacuuua cagcauggaa ugaaaaccac     3060

cagaaguucc uuccaacggu accggucgag uuuuccaaag gcuucucaaa gaaacguauu     3120

gaucaucgcc accaugccuu ggacgcucug gugaucgcgu gugcaaccaa ggaucacguu     3180

aauuuacuca acaaccagag ugccaaaagc gacacuaagc gguacgaucu gaagaaaaag     3240

cugaugaaau uugaaaaggu ggcuuacaau cacccccaaa caggagagag aauagaaagg     3300

gaggccccua aacaguuccu uaagccaugg gagaccuuua caauugacgc aaagaaccau     3360

cuagaaacua ucaucgucuc guucaaacag aaucuccgag ugauuaacaa ggccaccaau     3420

uauuacgaga aauauguaga aaaggagggc acgaaaguga aggaacgggu ugagcagaag     3480

gggaccaacu gggcuauacg caaacccaug cacaaggaga cagucuccgg aaaaaucgau     3540

cugccuagaa uuaaggugcc aaaagguaag aucuugacug ccaccaggaa gucucuggac     3600

accagcuuca auuuaaaaac uauuggcuca aucaccgaca cggggauaca aaagauccuc     3660

aaaaacuacc ugcaguauaa ggauaauaac cccgaacuug cauuuagucc ggagggaauu     3720

gaagacauga auaaaaacau cgagaaguuc aauaacggca aagcgcauca guccauuucu     3780

aaggugcggg ucuuugagcu ggguagcaag uucccuuugg ggcaauccgg caauaaaaag     3840

gaaaaauacg uugaggcugc caagggaaca aaccucuuuu ucgcaaucua ucaggauaaa     3900

aauggcaagc gaucauacga aaccauacca cugaacgagg ugauugaaag acagaagcag     3960

gggcuaagcg ugguagaccu gaaaggaguc gaugacuuuu accuuagucc caacgaucuc     4020

guguauguuc cuucugagga cgagcuggaa aauaucucca ugaucgauuu caacaagaug     4080

agcucggagc gcauuuacaa aguggucuca uuuacuggca aucaaaucuu cuuugugagg     4140

caggacauag ccacaucugu ugugaacaag gcugaguuca guaccuugaa uaaaauggaa     4200

cgugccauug augguacuau gaucaaggaa accugcguaa agcugcagau ugaccgguua     4260

gggaacaucu ccaaaauaug a                                               4281


<210>  3336
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3336
auguacaaca ucggccugga cauugggacc ggaagcgugg guuggugccu cacagaugag       60

aauggcuauc uucugaaggu caacagaaaa gggaauaacg gaaauacuua caggaacucc      120

gccuggggcg uucgguuguu cgaaucugcu gacacggcag ccgauugucg cauaaagcga      180

ucaacccgua gaagguauaa acggcgcaga acaaggauca uugaguuacg gaagaucaug      240

agugacauga uuaugcccau cgauccuaau uuuuacgcgc gccuggacga ggcuuuccua      300

uggaacgaag auaaaagcga caaggccaag gcaccauuuc uccuguucaa ugauaacggu      360

uaugacgaug ugaaauacua uacugacuac ccgaccauau accaccuucg aaagcaucug      420

uuggaaacaa aagagaaggc cgaucccaga uuuaucuauc ucgcucugca ccauaugaug      480

aaauacaggg ggcacuuccu guuugaggga caguccuucg aagcaauuga caauaucgag      540

gacaccuuua uugaacucga gcauuuagua aacguguaug ucaaggagaa ggaagauacu      600

gacaauaacg ccgagaauaa cgcucuguac caagaaauga aaaacuaucu ugccgauaau      660

aaggugaaaa acaaggacaa aaaggaguac aucacagaua ccuucauaaa agcggacuau      720

gauaauaagu acucuaagga auuggcagcc gcuguucugg gcuacgaguu uaacgugggc      780

auuaucguca augacaacuc gcuaacggau gaggacggga aagcccucaa ggcaaaauuc      840

gcugaugcca aguaugaaga gaaagaagag aagcugucag acacucuugg agaacgguac      900

uauauuaucg agacccugaa gaaaaucuac agcuggaagg uguugcacag uauucucggu      960

gacaauaaau aucuguccua cgcgauggua gauaaguaug agaaacacuc ugaacaguua     1020

aaggcccuga aguaccucuu ucauaaauac acaagccagg acgaguauuc cgaguucuuc     1080

caccaggaaa agaacaaaga aggcaaguac auaguuaauu augcaaacua caucaaaggg     1140

auuaagcguc uuucaaauga gacuaacaag aaauauaaua ccaagcaaca gcuguaccag     1200

aguaucauga aaauauuggg agagcgcgcu gccgaugacg aaguguauaa gaaaauucua     1260

gucgagaugg aacaggagac auuucuggag aagaucaaca auguggauaa cagcgcuauc     1320

ccuuaccaac ucaaccugau ggaaauggac aaaauucuua cccagcaggg cguguacuau     1380

aaggagcuga gagauaauaa ggaauugcuc cugaaaaugu uaacgucuaa gaucccauac     1440

uaugucgguc cccugaacaa uaacuccaau gggaacagga auuucgccug gaugacuaaa     1500

aaggacggaa aagagaacga aaagguuuac ccuuggaaug ugaaggaugu aguggacauu     1560

gaugucacag cagaggacuu uauaacccgg augacuaacu auugcacaua ccuaccaaau     1620

gagaaagugc uccccaagga aucacugcuu uaucagcgau acauguugcu ggaggaacuc     1680

agccaaaucc gcauugaugg cagaaaacug aguaaggagg acaggaaagc caucaucaac     1740

gaccuuuuca uuggcaaggg gcggguuaag gugucugaua aagacuuuaa ggaauaccug     1800

aauaaaguca acuaugugaa gguaaaugga aaagguuacg auguuaccgg cuaucagucc     1860

gacgaugggu ucgcuugugc guugagcucg uacaacaagu uucgcagaau acucggacau     1920

aucgacgagc agaacgagaa gaugauugaa gauaucauuu auuggaucac gguguucgag     1980

gacaaagaua uagucaagag gaaaaucaau aagcaauacg cagaccgucu gucugccgaa     2040

caguuaaaga agauucugaa acuaaaguuu aagggcuggg gucgguauuc cucaaaacuc     2100

cugaacggga ucaagggcga uaugggaacc acaauuaucg agaugcuuga agacgcugau     2160

gagcgauucg ccuacugcga cuacuguccu aauuuuaugc agauaauuaa caaagacgag     2220

aagaucaaac agauuaucga ugacaauaga ccaagguaug auggcacuaa ggacuugcug     2280

gaugugauac aagacaugca caccaguccg gcaaaccgcc gggggaucug gcagacaaug     2340

aaggccauug aagagaucau ugaauacaug ggagagaaac cccagcagau cuauauagag     2400

uucgcuagag aagaugaccc uaaggccaaa aauaagaggg cagauagccg caaacgagcg     2460

auugacaagg cucucaagaa acugaaggag gaugucgugg acgaauacaa cgagaaaguu     2520

uauaaggaau uaaaacaaua cgagaagcgg cuggacgagg aaaaggugua ucuuuacuuu     2580

augcagaaug guaaagauuu guacuuccca gacaaggagc ucaaccugga ugaacuagag     2640

aaacuggaaa ucgaccacau cauucccuau ucccucucug augacgauag ccuggacaau     2700

aaggcccuug uauugaaagg caacaaucag aacaagggga auaagaucgu caaagaggca     2760

uucccucagc uguuuucaaa caguggaacu gugaacuacu ggaagaaucu caaaaaggcc     2820

ggcuuaauuu ccgagaaaaa guauaacaau cugcagaaaa acaauguuga ugacauacuu     2880

accaaggguu ucaucaaccg ucaacuggug gaaacaagac agauugucaa gagcguggcu     2940

aauuugaucc ucgauuacua uaacgagcag auugacguaa ucgaagugaa agccucucug     3000

ucaaccagug uuaggaagau gcuaacuuac gagaaaaagg auaauaacgg guuuuggaua     3060

gaaaauaaag acaacugcau guucuauaag aaucgcgaca ugaacgauua ccaucacgca     3120

caugacgcuu accuggccaa uaucauuggc cucuauaucc aaaagaacua cccacggcug     3180

cagaaagagc uuaauuauag ccaguacaga aggauuuggc gaaaguauua cgagaacacg     3240

aaaaacaaua acggagucaa uugguuugcg acauugggca aguucuccuc uaacaaugaa     3300

gaggccgauu gguaugggca agacaucaua gcuuacaugc gcaaaaucuu uuguuaccgg     3360

gaugugcuga uuucgaagaa gguggaagag aacaccggag cauucuauag cgagacuaaa     3420

uaccccagag aagacaaggc cgguuccaaa uuagucccgc ucaagcaggg caauaacaug     3480

cguggggcua auaaccugaa agagcuugau gccaggaagu auggaggcua caaggguggg     3540

gaaaaagcau auuuuguucu ggugaaguac ugcaucgaga aagcgcuaaa gaaaaaggug     3600

aaaaaggaau aucacaugga guucguagag auuccugucu acauagcccg cggcaucaag     3660

aauaaagaca uuaaccucua cgauuaugug ugugacaucc ugaaugaagc uaacaaaaau     3720

ucaguugccg auguggagau uuugcggaac aaagucccca aguaccagau gaucauagga     3780

gaaaacggcg aggaauauua ccuggugagu gcaaccgagg uuaucaauuc uaaacaguuu     3840

guguuagggg gagcuaacca acaguauaau agacuccuga acuacauuaa guauggcaag     3900

aaugacaaau ggcaguacau ccagacagag cuucuggaug aacaauugau uggucucuac     3960

aaccugcuaa ucgccaagau aaaagacgag uauaaggggu ucagcaaaga agcaguaagg     4020

auucaggaga auaacuccuu ugacaagcug gaugucaagg acaaaaagga guucaucgcc     4080

gaaaugauca aacuugugca gccagauuca gacuacccuu aucucggaaa guacgcuacc     4140

ggccugagca aucgaauggg ucggaaaacu ggcgagaagg uggggaagaa aauuacauug     4200

gucgauaagu cuguuacggg acuguaugaa cgcagaacca cuuucgaguu agaagacgau     4260

uccaguacaa aaagcugcug a                                               4281


<210>  3337
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3337
augaagaaca uccugggccu cgaccuuggg accacaagca uuggauucgc ccacauagug       60

gaggaugaaa auaaagagaa guccgaaauc aaagagcugg gugucagaau uguuucuuug      120

acuacggacg aacaaucaga uuuugagaag ggcaaaagua ucaccacaaa cgcuaauagg      180

acuuuaaagc auggggcacg gcugaaccua gaccgcuacc aacagcgacg uaaguaucuc      240

auugaucugc uucagaaagc caaucugauc acccccagcu ccauauuggc ggagaacgga      300

aagaauacaa cccacucuac uuggcagcuc agagcuaaag ccgugacaga aaggaucgag      360

aaggaagagu ucgcacggau ucugcuggcc aucaacaaaa agcgcggcua caagucguca      420

agaaaagcua agaccgagga cgaaggucaa gcaauugaug ggauggccau cgcuaaaagg      480

cucuaugacg agaauuuaac gccuggacag cugagccuuc aguugcugca gcaaaacaag      540

aaacuacucc cagauuuuua ccggagugac cugcagaagg aguucgaucu uguauggaau      600

uuucagaaac aguucuaucc ggacauacug acugauaucu uuuacaagga auugcaaggc      660

aagggcaaag acgccaccuc caaggcguuc ucuaaacgcu aucauuuuga cacaacugaa      720

aacaagggga gcaaagaguc cgugcgacuc caggcauacc aguggagagc cgaggcuauu      780

ucaaagcaac ugaguaagga agaggucgcc uacguguuaa ccgaaauuaa uaacaaucug      840

aacaacgcaa gcggauaucu cggugcuauc ucugauaggu ccaaagagcu uuacuucaau      900

cggcagacag uuggccagua ucuguacgcc aaguugcagg aaaaccguca caauucauug      960

aaaaacaagg uguuuuaucg ccaagacuac cuggaugagu ucgagagaau cugggaaacc     1020

caggcgagcu uucauaaaga gcucacggac gaacugaaga agcagauuag ggaugucgug     1080

auauucuauc agcggaaacc caagagucaa aaagggcuua ucucuuucug cgaguuugaa     1140

uccaaggaga uugagaucga aaaagacgga aagacuauaa caaagaauau uggcgcccga     1200

guaguuccua aaagcucgcc acuguuccag gaguuuaaga ucuggcagau cuugaacaau     1260

gugauuugua aacgcaaggg uaucaggaaa aagaaaauuu cugcaaagac cacucagcuc     1320

gaucuguuaa acgaauccuc acaaacaaua uucagucugg acauggagug caagcagcua     1380

cucuuugaug agcugaaucu uaaaggggac cuaaagagcg auaaaguccu gaagcuccug     1440

ggauacuccc cacaggaaug ggagaucaac uacaaucagc uugaaggcaa cagaacccaa     1500

aaagcucugu augaggccua cuugaagauu guggaaaugg aggcucacga cgugaaggac     1560

auccuccaga ucaaaucugc caaggaugac uggagccugg augagucacc uuuaagugca     1620

uccgaaauuc gggagaaagu caaggccaua uuccagacgc ugggcaucug uaccaaaauu     1680

cuauauuuug acccacuccu gcccguuaag gaguucgaag aacaagauag cuaccagcuu     1740

uggcacuugc uguauucuua cgagucagac gauaguacaa gcgggaauga gacucucuau     1800

cgcauccugg aaaagaaaua cgcuuuuaag agagagcaug cgaggauuuu agcaaacgug     1860

gcccugcagg acgauuacgg aucccuuucu accaaagcua uccguaagau auauccuaau     1920

aucaaagaaa accaguacuc gacagccugc gagaaggcag guuauaagca cagcaaauug     1980

ucccucacua ccgaggaacu ggaggcccgg gaacuaaaga acauuauccc acugcucaaa     2040

aagaaugcuc ugcgaaaccc gguaguggag aaaauucuua aucaaaugau caacgucgug     2100

aaugccuuga uagaaaagaa cucagagaga gacgcagagg gcaaaauuac aaaguacuuc     2160

cauuuugaug aaaucaggau ugagcuggcg cgcgaauuaa agaaaaaugc ucagaagcgg     2220

uaugagauga cccagaacau caauaaagcc aagcucgaac accagaaaau aagugagauc     2280

cugcaaaagg aguucgggau uaagaacccc acuaaaucug acaucauuag auacaggcuu     2340

uaucaggaac uggagcacaa uggauacaag gaauuguaca cgaacgcacc uaucgcccgc     2400

gauaugcucu uuagcaaaaa uauagagauu gaacauaucg uuccaaaggc ucgaguguuc     2460

gacgacuccu ucucaaacaa aacacugacc uuucaccgga ucaauagcga uaagggcgag     2520

uauacugccu ucgacuacau uaccucucua aacuccgagg aggaacugaa ccaguaucuc     2580

acacgugucg aaaaugcaua caagaccaaa aguaucagcc ccacuaagua uaaaaaccug     2640

cuuaagaagg cuucugagau uggugaugac uuuauaaaua gagauuugag ggacacacag     2700

uacaucgcca aaaaggcgaa agaaauucug uuccaaguga cgaagaacgu auuauccacc     2760

ucagggagua ucacugaucg ccuccgggag gacuggggcc ugguugaugu gaugaaagag     2820

cuuaauaugc cuaaguauca gagccuggga cuaacagaag ucgaggaaag aaaagacggc     2880

aacaagguga ccgucauuaa gaauuggaca aaaaggaacg aucaucgaca ccaugccaug     2940

gacgcucuca cuguggcauu uaccaagcca ucguacaucc aguaccugaa ucacuugaac     3000

gcccgcaaag augagaauaa caagaauuau uccguuauac uggcuaucga ggaaaaagag     3060

acgauuaagg ugcccaccaa caaugggaaa aacaagcggg uauucaucga accgauuccu     3120

aacuuuagac aagucgccaa gaaacacuua gaggaaaucu ucauaucuca uaaggcuaaa     3180

aauaaggugg uuacuaaaaa cacaaauaag cccgcgggaa ccgacaagca gcagauuaca     3240

cucacuccaa ggggucagcu gcacaaagag accaucuacg gcaaguauca auacuauauc     3300

aacaaagagg aaaagauugg ggugaaauuu gacgagcgua cgauagccaa ggucagcaau     3360

ccuguguacc gcgaagcacu ucugaagcgg uugcaggcca acgauaauga ccccaaaaag     3420

gcauucgcug gcaaaaacgc ccucucaaag aauccaaucu aucuggauga gaguaaaaca     3480

aagacccuac ccgaaaaggu aaaccugacu uaccuugagg aggacuuuuc cauuagaaaa     3540

gauaucucuc cugacaauuu caaggaucuc aaaagcauug aaaaggugau cgaccaggga     3600

guuaaaagga uacugaucaa gcgauugcag gcauacgaua acgaccccaa aaaggccuuu     3660

gucgaucugg agaagaaucc gauuugguua aacaaagaaa aggguaucgc uauuaaacgg     3720

gugacaaucu ccggggugaa uaacgcgcaa ccccuccaua uaggcaagga ccaccuggga     3780

aaaaccacuc uuaauaagga gggcaaggaa auuccugucg acuauguuuc aaccgggaac     3840

aaccaccaug uggccaucua ccgcgauaaa gagggaaauc ugcaggagca gaucgugagc     3900

uucuucgacg caguagucag agcucagcaa gguauuccaa ucauagauaa gacauauaaa     3960

caggccgaag gcuggcaguu uuuguucacg augaagcaga acgagauguu uguguucccc     4020

aaugccacca cuggguuuaa cccugcugaa auugaccucc uggaucccaa aaauaagaag     4080

cuaaucaguc caaaccuguu caggguucag aaaauugcaa caaaggacua cuuuuuccgg     4140

caccaucuug agaccaaugu ggagacagau aacauccuca aaaaugucac uuggaagcga     4200

gaaggacugu cuggcuugaa agacauagug aagguuagaa uuaaccacuu aggugauauc     4260

guguccaucg gggaguauug a                                               4281


<210>  3338
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3338
augaagaaca uccugggccu cgaccuuggg accacaagca uuggauucac ucacauagug       60

gaggaugaaa auaaagagaa guccgaaauc aaagagcugg gugucagaau uguuucuuug      120

acgaccgacg aacagucaga uuuugagaag ggcaaaagua ucagcacaaa cgccaauagg      180

acuuuaaagc auggggcucg gcugaaccua gaccgcuacc aacagcgacg uaaguuccuc      240

auugaucugc uucagaaagc aaaucugauc accuccucuu cgauauuggc cgagaacgga      300

aagaauacaa cccacucaac uuggcagcuc agagcgaaag cugugacaga aaggaucgag      360

aaggaagagu uugcccgggu acugcuggca auuaacaaaa agcgcggcua uaagagcagu      420

agaaaagcca agaccgagga cgaaggucaa gcuaucgaug ggauggcaau ugccaaaagg      480

cucuacgacg agaauuuaac gcccggacag cugucccuuc aguugcugca gcaaaacaag      540

aaacuacucc cugauuucua ucggucugac cugcagaagg aauuugaucu uguguggaau      600

uuccagaaac aguuuuaccc agacauccug acugauagcu ucuauaagga guugcaaggc      660

aagggcaaag acgcuaccuc caaggccuuu ucaaaacgcu accauuucga cacagcggaa      720

aacaagggga gucgagagag cgucagacuc caggcauauc aguggagggc cgaggcuaua      780

ucuaaacaac uguccaagga agagguggcc uacguuuuaa cugaaauuaa uaacaaucug      840

aacaacgcau caggauaccu cggugcuauc agcgaucgga guaaggagcu uuauuuuaau      900

cgucagaccg ugggccagua ccuguaugcc caguugcaag aaaacaaaca caauucucua      960

aagaacaaag ucuucuaccg ccaggacuau cuggaugagu uugagagaau uugggaaaca     1020

caggcguccu uccauaagga gcucaccgag gaacugaaaa agcagaucag ggacguggua     1080

aucuucuacc aacggaaacu uaaaagccag aaggggcuga uuucguuuug cgaguucgaa     1140

ucuaaagaga uagaaaucga gaaggaugga aaaacgauua cuaaguccau cggcgcccga     1200

guugugccga agucaagucc cuuguuucag gaguucaaaa uauggcagau ucucaauaac     1260

gucacaugua agcgcaaagg uaucagaaag aaaaagagcu ccgcaaaauc uaccgaacug     1320

gacuuauuua augagccuag ccaaacuauc uucucacugg auauggaaug caagcagcua     1380

cucuuugagg aacugaaccu uaagggggac uugaaaagug auaaggugcu gaaacuccug     1440

ggauauuccc cacaggagug ggaaauuaau uacaaccagc uugagggcaa uaggacacaa     1500

aaggcucugu acgaggccua uuugaaaauc guggaaaugg aggcucacga cgucaaggau     1560

auucuccaga uaaagagcac caaagacgac ugggcccugg aagagucucc cuuaucagca     1620

agugaaaucc gggagcgcgu uaaggccauu uuccagacgc ugggcaucug uaccaaaauc     1680

cuauacuuug auccucuccu gccagugaag gaguucgagg aacaagacag cuaucagcuu     1740

uggcacuugc uguacuccua ugaaucugau gacucgacaa gcgggaacga gacucucuac     1800

aggauucugg aaaagaagua ugcuuuuaag agagagcaug cgaccauauu agcaaaugua     1860

acacugcagg augacuacgg aucccuuuca acuaaagcca uccguaagau uuaccccuau     1920

aucaaagaga accaguacag uaccgcuugc gaaaaggccg guuauaaaca cucuaaguug     1980

agccucacaa ccgaggaacu ggaggcacgg gagcuaaaga auauuauccc ucugcucaaa     2040

aagaacgccc ugcgaaaccc aguggucgag aaaauacuua aucaaaugau caacgugguu     2100

aaugcuuuga uugaaaagaa cuccgaaaga gaugccgagg gcaaaaucac uaaguacuuc     2160

cauuuugacg aaauuaggau cgagcuggca cgcgagcuca aaaagaaugc gcaggaacgg     2220

uaugagauga cgcagaacau aaauaaggcu aaauuagaac accagaagau uucagggauc     2280

cugcaaaaag aguucggaau uaagaacccg acaaaaagcg auaucaccag auacaggcuu     2340

uaucaggaac uggcccacaa uggcuacaac gacuuguaca cuaauaccua uauacccaag     2400

gagcuccugu ucucuaagaa caucgauauu gagcauaucg uguccaaagc acgcgucuuu     2460

gacgauaguu ucagcaauaa gacacuagcc uuucgagagu ucaacauuaa aaagggugaa     2520

ucuaccgcuu uugacuacau cacuucccug aauaacgaag aggaacucaa ccaguaucug     2580

aaucgggugg agaacgccua caaaacaaag aauauaucac cagcaaagua uaaaaaccuu     2640

uugaagaaag cuagugagau uggggacggc uucaucaauc gugaucugag agacacgcag     2700

uacaucgcca agaaagcgaa ggaaauuuua uuucaaguaa ccaagaacgu ucucagcacu     2760

ucgggaucca ucacagauag gcugcgcgag gacuggggcc uuguggaugu caugaaagaa     2820

cugaauaugc caaaguauca gucucuaggg cucaccgagg uggaggaacg gaaagaggga     2880

aacaagguca cagugauuaa aaauuggacu aagagaaacg accacaggca ucacgccaug     2940

gaugcucuga ccguugcauu cacgaagccc agcuacauac aguacuugaa ucaucugaac     3000

gcccgaaaag acaagaauaa caaaaauuau ucagugaucu uagcuauuga agagaaggaa     3060

accaucaaag uaccuacaaa caacgguaag aauaaacgcg ucuuuauuga gccaaucccc     3120

aacuuccggc aaguggccaa gaagcaccuc gaggaaauau uuaucaguca caaagcaaag     3180

aauaaaguug ugacuaagaa caccaauaaa ccggcgggca cagauaagca gcagauuacu     3240

cugaccccua gagggcagcu ucauaaggag acggucuacg gaaaauauca auacuaugug     3300

aacaaggaag agaaaaucgg cguaaaguuc gacaaagaaa caauugccaa gguguccaau     3360

cccguuuaca gggaggcucu guugaagcgu cuccaggcca acgauaauga cccuaaaaag     3420

gcauuugcug guaaaaacgc ccugucuaag aauccuaucu aucuagauga ggcaaaaacc     3480

aagacucuga cagaaaaggu caaccuuacc uaccucgagg aagacuucag cauacgcaaa     3540

gacauuuccc ccgauaauuu caaggaccug aaaucaaucg agaaggugau cgaucagggg     3600

gugaaacgga uuuugauaaa gagacugcag gacuacgaua acgaccccaa aaaggccuuu     3660

gucgauuuag aaaagaaucc aaucuggcuc aacaaagaga agggcauugc uaucaaaagg     3720

guuacuauua gcggagugaa uaacgcgcaa ccucugcaca ucggcaagga ccaucuuggg     3780

aaaaccacac ugaacaagga gggaaaggaa auaccagugg auuauguaag uacggguaau     3840

aaccaccacg ucgccaucua ccgagacaaa gagggcaauu ugcaggaaca gauugugucu     3900

uucuuugagg caguuguggg ggcucagcaa ggacucccga ucgucgauaa gaccuauaaa     3960

caggccgagg gcuggcaguu ccuguuuacu augaagcaga acgaaauguu cguguuuccc     4020

aaugaggcca cagguuucaa cccugcugaa auugaccuac uggaccccaa aaauaagaag     4080

caaaucuccc caaaccuuuu ucggguucag aaaauagcaa ccaaggauua cuucuuucgc     4140

caucaccucg agacaaaugu ggacacugau aacguacuga aaaauaccac guggaagaga     4200

gaaggguuga gcggcuuaaa agacauuguc aaggugagga ucaaccaucu gggagauauc     4260

gugucgauug gcgaguauug a                                               4281


<210>  3339
<211>  4284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3339
augaagaaaa uccugggccu cgaccuuggg accagcucca uuggaugggc ccuggugaac       60

gaggcugaaa caucugagga aaagucaagu auaaucaaau ugggugucag aguuaauccc      120

uuaacugugg augagcugca gaacuucgaa aagggcaaaa gcauuacgac caaugcagac      180

aggacagccg ggcgguccau gcgccgaaac cuacaacguu acaagcucag aagggagaau      240

cugaucgagg uacuuaagga auauaacuuu auuacugaug agaugauccu gucugaaaau      300

ggaaaccgga ccacauucga gaccuaccgc uugagagcga aagcugccgu ggaggaaaua      360

ucgcucgagc aguuugcaag gguccugcug augaucaaua agaaacgggg cuauaaguca      420

agccgcaaag ccaagggugc ugaaaacggg acucucauug acggaaugga gguggcaaag      480

aaaauguacg augaaggcau cacaccuggc gaguuaugcc ugcagcuuuu gaccgccggg      540

aagcgauauu ucccagacuu uuacagaagu gaucugcagg cugaguucga caggauuugg      600

aauuuucaaa aacaguucua uccggauucc cuaaucgaca agguuaaaga ugaagugcgg      660

ggaaagaaca aaucucagac gugggccaua cucgcgaagu acuuugucug gaaggaggug      720

gaaaauagcu ggaacgagga agaggcacag acucgucgcg uagagaaaga auacagacug      780

guugguauua agaggggcgu gaaacgggag gaacuuaagc uggagaauuu ccaauggcga      840

gucaaagccu uguccgaacg caugaacccc gaggagcucg cuaucgugcu gcaggaaauu      900

aacgagcaga ucucaaauag uagcggguau uuaggagcca ucucugacag auccaaggaa      960

cuguacuuua acaggcagac cgugggccaa uaucagaugg caguccucga uaauaacccu     1020

aauauugguc uucggaacau gguuuucuac cgccaggacu aucuggacga guuuaauaca     1080

auaugggaga agcaagcuga auuucacaaa gaguugacug aagaucuaaa gaaagagauc     1140

agagacauug ugaucuucua ccagaggcgu cugaagucac agaaagggcu cauaaacauu     1200

ugugaauuug agcggcgaca gaucgaggua gaaaucgaug gaaagaagaa aauuaagacc     1260

aucggcagca gagugauucc aaggaguucu ccccuguucc aagaguuuaa aauauggcag     1320

acacuuaaug acaucgaagu cuccgugcug ggcguuaaga acaaacgcaa gaagcaggau     1380

gacaauagca ccacguugcu cgauucggcc gagaacauug acucucugaa auuaaaugug     1440

ucccggccuc uggaugcgga cgaaaaguca cuacucgcca aagagcuguu caucagagau     1500

aaacuuacua agagugacgu cuugaagcug cucuuuaaca auccacagaa ccugaaccuu     1560

aauuucaaaa acaucgacgg gaauaggaca ggaagcgcac uguaucaggc uuuuuccaag     1620

auuuuggaga uaucugguca ugaaagcauc aacuucaaga aaucagccga ugagauugug     1680

gaacaaguaa agaccaucuu uagugcucuc ggcuggaaua cugagguucu gugcuucgac     1740

uccgaaaaag aguuagauaa gcagcccuac uuuaaacugu ggcaccuacu cuacagcuuc     1800

gagggggacu cuacaccuac cggagauggc aaccugauuc agaagaucgc cgaccuuugu     1860

gguuuugaaa aggauuaugc aucaauauug gccaauauca cguuccaaga ggacuacggg     1920

agucugagca ccaaagcuau ucgcaagauc cucccacauc ugaaagaugg caaccaguau     1980

gacguggcgu gcgaauacgc aggauauaag cacuccaaau cuucguuaaa gaaggaggag     2040

auuaaaaaua agguccugaa agauaaacuu gaauugcucc cgaagaacag ccugcgaaau     2100

cccguggucg agaagauccu aaaccagaug gugaauguua uaaacgccau uaucacaacu     2160

uacggcaagc cugacgaaau ucggaucgag cuggcucgug aacucaaaaa gaaugccaaa     2220

gagagagagg aacugaccaa guccauagug gauacaacua aaacccauga ggggaucagg     2280

gcaauucuuc agaacgacuu cggaauguca cacguaaguc gcaacgccau cauucgguau     2340

aaguuguacg aggaacugaa agacaauggu uacaagacau uauauucuaa caccuacauc     2400

ccaaaggaaa aacucuuuag caaggauuuc gacauagagc acauuauccc ccaagcuaga     2460

cuguuugaug acuccuucuc aaauaaaacu cuugagguca agagcaucaa cauugaaaaa     2520

ggcucuaaga cggccuauga uuuuguggag gaaaaguacg gggagcaggg acugcaggaa     2580

uauuugaaua ggugugaggu ucucuuccga gacaaaaaga caaaacugcg caagcuaaaa     2640

augcaagagu ccgaaauccc ugagggcuuu auugaucggg accugagaaa cacccaguac     2700

auagcaaaga aggcgcucag uaugcugaau gaaaucugca ggcguguggu cgcuacuacc     2760

ggugccauua cagaugagcu ucgcgaggac uggcaguugg uggauguaau gaaagaacug     2820

aacugggaga aguauaaagu guuagggcuc guugaauacu ucgaggacaa ugauggccgg     2880

cugaucggaa gaauuaagga cuggaccaaa aggaacgacc aucgacacca ugcaauggau     2940

gcccuuacug ucgcuuuuac aaaggacgug uucauccagu acuuuaauaa caagaaugcc     3000

agccugaaac cagauacgaa cgaagcagcu auacggaaua aguauuucca aaacggcaaa     3060

gccauucccc cgaugucucu agacgaguuu cgcgcggagg ccaagaaaca ccucaacgau     3120

acccugaucu ccaucaagac uaagaauaaa guggucacaa uuaacuugaa uagaaccaag     3180

aaaaagaacg ggaucaauaa aaagcagcag cagacaccua ggggacaacu gcacuuagaa     3240

acuguuuacg guucacauaa acaguaugug gugaaggagg aaaagguaaa cgcuaguuuc     3300

gacgcagcca aaauagcuac cgucaauaag cccgccuacc gggaugcacu ccugcgaaga     3360

cuucaggaga acggcaauga cagcaaaaag gcguucacgg ggaaaaacuc gcuggauaag     3420

cagccagugu gguuggacgc caagcaaaau gaauguguuc cugagaaagu gaagaccguc     3480

augcucgaga caguguauac uguucgcaaa cccauugaug aaaagcugaa cguggacaaa     3540

guagucgacg cuaggauccg uaagcuacug caggaucgga ucaaggagua cggaaaugcc     3600

aaauccgcau uugcuaaccu ugacgaaaau ccaauuuggc ucaacaagga gaaaggcaua     3660

accaucaaga gagugucugu gagcgguauu ucaaaagccc agagucugca cgaaaagaag     3720

gauaaagacg ggaauuucau cuuggauaag gacuccaggc gcauucccgu cgauuuuguu     3780

aacacaggca acaaucauca cguggcaauc uaucgaaaac cuguacugga caagaaagga     3840

caagccgugu ucgaugagaa cggcgaguua aaguuugaac ucgaggaagu cgugguuucu     3900

uucuuugacg cugugacuag agucaaucug agccagccaa uaaucgauaa agacuacaag     3960

cgguccgagg gguggaaguu ccuuuucuca augaaacaga acgaguacuu cguguuuccg     4020

aaugaaaaga ccggauucga ucccaaagag auugaccugu ugaacccuga aaauuaugcg     4080

cucaucagcc caaaccuguu caggguucag aagauugcca guaaagacua cguguuucgc     4140

caucaccuag agacggucgu agcagaucgu aaggaacugc aagagaucac auggaagaga     4200

auaucuuccc uuagcauucu caaucggauc aucaaaguga ggguuaacca cauuggccag     4260

aucgugauga uaggugagua uuga                                            4284


<210>  3340
<211>  4284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3340
auggccaagc ccaaagacua caccaucggc cuggauauug ggacaaacag cgugggaugg       60

gucguuacug acgaucagaa uaacauacuc agaaucaagg guaaaaaggc uauuggcgca      120

aggcuuuucg ccgaggggaa aguggcggcu gaacggcgcu ccuuucgaac gacccguaga      180

aggcugucuc ggcgcagaug gaggaucaag uuguuagagg aacuguucga caaggagauu      240

gccaaaguag auccuucauu uuucgcacgg cuacacgaaa guuggaucag cccaaaggac      300

aaacgcaagc gauauuccgc cauaguguuu ccgucucccg aggaggauaa aaaguuccau      360

gaaucguacc cuacaaucua ucaccucaga gacaagcuga ugaaagauga ccaaaagcau      420

gauauuaggg agaucuacau ugcuguccac cagaugauca aagcacgggg aaauuuucuu      480

caugacgaau caguggagac uuaucguagc ggcaugaguu cccugggugg gcgcucugag      540

agaaccauau ugagcguuca gacacucgaa gaacugaacg aucuguucgc cgaaaaugag      600

ggaaccgaag aggcuaagcu caacguggcc uccgcggagc agauuaauga caucuuaacu      660

ggcggccacc ugaacgcaga cucacaaaaa gaaauuggga aucuuuugcu gccaaguagc      720

uuucccucuu ucgaugacaa ggccaaagag aagcagguca agaaacuaau caagaacgug      780

gcuacaaaua ucuccaaagc cuggcucgga uacaaggcag auuuuucaac cauucugaac      840

cuugcuaaug uagacaaaga ucagaagaag auauucgccu uugcgcugca ggguggcgac      900

gaagaggaua aaguucaaga auuggagagc cuccuggagc agagucagac ggacaucgug      960

gauagguuaa uugaaauccg gcacgccaua guccugucug agauugugcc uguggggaug     1020

acucucuccg aagcaaugau cgacaaguau gaucagcaua aagaggaccu uaucacccug     1080

aaggcuguca uucgaaacac aaaagacaag aagaaagccg cuaaguugca agccaucuac     1140

gaucuauacg uuaauaaccg ccacgcagac cuggccaaag cuaugaagcu cacuggaauu     1200

aaaaagagaa gcgaacugcu ugauccagag gagcugaaga aagcgauauc gucuuugcuc     1260

gacgauuccc ccgaagcagu ggagaucaag cagaugcugg aagagaaaac cuucuuaccu     1320

cugcagaggu caaacaauaa cggcguaauu ccaaaucaac uacaucaggu ggagcucgac     1380

gaaaucauca agaaacagag uaaguauuac ccguuccugg ccgagaaaaa ccccgaugaa     1440

agcgaggaag cucagaagaa ggccccuaca aaacuugacg cauugcugac cuuucggguc     1500

ccauauuacg ugggucccau gauuacgaag gaggagcaag aagcccagga ugggcacucc     1560

uucgcuugga ugguucgcag agacccucag gaucacgagg ccauaacucc auggaauuuu     1620

gagaaaaagg uggacaaaau ggcaucugcg acacaguuca ucaagaggau gaccacuaag     1680

gauacauauc uccugggaga ggacguccuu cccgcuagcu cacugaaaua ccaauuguuu     1740

accgugcuca acgaacugaa uaacuuacgu guaaauggca agaaacugac gagugacgag     1800

aaggagcagg uuauugaagg ccuauucaaa aagcagaaga ccgugaaagc cgauaaguuu     1860

gucaaauauu ggcaggcaaa gcauaucggg gccgacauua aagugaaggg acucuccgau     1920

ccgagcaagu ucaacucuac aaugucaacu uacaucgacu uuaaaaagau auucggugau     1980

caacugaaug acgucaaccg gcagaaugau uuugagaaaa ucauugaaua caguaccauc     2040

uucgaggacc gaaagauucu ugaagauaaa uugagagcug acuuugccug gcugagcgag     2100

gagcagauca agcagcucug caggcugcgc uuacaaggcu gggggcggcu guccgauaaa     2160

cuuuugacaa gacucacuga cgcagaugga cagaacgugc uggaaaggcu auggaacucu     2220

aaugacaacu ucguucagau agugucggac ccuaauauua aggcuaagau cgagcaagaa     2280

aaccagaaac ugcuccgcga uggccagagc gacgaugccg uagaguccau ucuggacgau     2340

gcguauaccu caccccagaa uaagaaagcc auccgacaag ucaugcgggu gguucacgac     2400

auagugaagg cuugugaugg uaacguccca gcaaaauucg ccaucgaguu ugcucguagu     2460

caggaagacg auccuaagag aacaaccgcc agggugaauc agaugcagaa gauuuacgac     2520

caaaucucug augagguagu gagccagggg guuaaagaac agcuuuccgg caugaaguca     2580

uugaaagacc gcuauuaccu guauuucaug cagggaggcc gggacgcaua cacuggggcg     2640

aaguuaaaca uugauagacu cagcgacuau gauaucgacc auauaaugcc ccaaucuuuu     2700

gucaaagaug acucccugga uaauagggug cuuguggccc gagcugucaa caaucagaag     2760

agugacaagg uuccagcccu guuguucgga aacaaagugg uggcagaucu cgguauuacg     2820

guacgcgaga ugugggacaa guggcagaaa cugggcauga ucagcaagcg gaaacuaucu     2880

aaucugcuca cagaucccga cgcucugacc gaauacagag cccaaggguu uaucaggcgu     2940

cagcuugucg agacuuccca ggugauuaag uugaccgcaa caauccugca gucagaguuc     3000

ccugauagua agauuauaga aguuccagcc aaauacaaca gcaucgugcg caagcaauuu     3060

gacuuauaua aaucgcggga ggucaaugac uaccaccaug cuauugaugc guaucucucc     3120

accaucgugg gaaacuaccu guaucagguu uacccgaauc uuagaaggau guucguguau     3180

ggcgaauuua agaaauucuc uagcaacgcc gaggaaucag cauacgacgu aguccgacgg     3240

gugaagagua ugaacuuucu ggaugaccua cuccgcggua cucacgggga uaauauuuac     3300

ugcagaucca caggcgagau cguguucaac aggaaugaca uaaucucuaa gcugaaacag     3360

gcuuauagcu uuaagcagau guuggucacg caagagguuu ucaccaaaaa guccgcccug     3420

uucgaucaga cuguguaccc cucaccugaa cgggacagca aaaagcgaag uggauuaauu     3480

ccacgcaaaa agggcaugga uacagagauc uaugggggau acucugguaa caaggacgcc     3540

uauuuuguac ucgcugaagc agugaaagag aagggccaca cccugcagau ugucggggug     3600

cccaucagag cccuuaauac acugaaaaac uccgcgaauu acagcgaaaa guugcucgag     3660

auaauuaaac cucagguuau guucaacaag gauacuggaa agccaaucaa aggcaucaag     3720

gacgugaaaa uucugaugga uaagauaccc uguaggcaac cgguccuaga gggugaaucg     3780

uauuacaugc uggcuucauc uaaauaccgu uauagucuua agcagaucuc ccucagccag     3840

augucuauga aguacauucu ggacuauauc gacgauccua auuuuaacaa acaugagaug     3900

auuaauaucg accagcaaga ugaaaaggag ugcuugcuga gcguguacga cgagauauua     3960

gagaaaaugg auaaguaucu cccacuguuc gacauccggu ccuuuagaaa gaaacuucac     4020

gaugggaggg acgccuucau ugcacugccc guugccucag aagaaaagaa accuggcaag     4080

guggauguca uccgcaaagu auugaaggga cuccaugcua acgcagacau uaccaaucug     4140

gccgagcuag gcuuugggac ggcggcucug ggagcccuug ugaguaccgg cgguaucaaa     4200

auaagcgaug acgcaguuuu cauuuaccag ucucccacag ggcucuuuga acgaagagug     4260

aaggucuccg accuguugaa auga                                            4284


<210>  3341
<211>  4287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3341
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggauccgc cuacgugcug       60

gaggcugaaa agucugcaga gcagacuaaa auagucuauu uggguacgag aaucauuuua      120

ucaagugccg augcggaaaa cuucagcaag ggccuguccu gcucucccac cacagacagg      180

acuuuuaaac ggucgaugcg ccgacgucua caaagauaca agcagaggcg gucaagccuc      240

auccagcagu ucaagaaaau ugggaucaua accaauacag agcaaaucau ucgcgaaccu      300

aucagugaga cccacgagcu gcuuagaauu agguccaagg cuguuucuga acgggugagc      360

cuggaggaau uugcccgcau cuugcucacu auaaacaaac gaagaggaua uaaguccaau      420

aggaaaacau caaagaguac cgaggaggau ggcaagcugg uagacgcaau ggccgugacg      480

aaagaacuga uuaagcucaa aaagacucca ggucaguaca uguucgagcg guuuaaacgu      540

gggaaguaug cugaaaucga uuucuacccg uuugacuuaa uuaacgagcu gaaacagauc      600

ugggaagucc agggacaaaa guaucccgau gugcuuaccg aggacuugcu gagcucucua      660

cucaagcaga aaacaggcaa ggcaaaagag cugaucuacu ccaagucaga ucuuaauccu      720

gccgaaguuc caauugacua ccagaaaggc aagaagcugc gcaaaacuau auugaacaag      780

uauuucgcuu acgagagcag aagucucgcc gcgaccaaua ucauucccgu ggaacagguc      840

aucacagcaa uaguggccau uuguaaacaa aucaacaauu cuuccgggua ucugggagcu      900

aucggugaua ggagccgggu auuauacgcc aacaaucaga ccauuggcca guaucuguac      960

gagcgaauca aggcaaaccc ucaugaccgc auuagagggg aaguuuucua uaggcaagau     1020

uaccucgacg aguucgaccg gauaugggag ugccagcaga auuacuaucc agcuauccuu     1080

acgaacgauc gcaaacaguu uauuagaaau aggaucaucu ucuaccaacg uccccugaag     1140

ucgaagaaau cugaauuggc cuuuugugag uucgaauccc gggaggugga acacaacgga     1200

aagaaauaua cugagggccu acaugucgcg ccuaagucaa guccaguguu ccagcacuuu     1260

cgacuguggc agagacucgg ucagauugag gugagcccgc ugaggaaaug gaacaagcaa     1320

uccaaugaac uuuucgagca gcagaacgac acagaucagg aacgcauacu gaauaccgag     1380

gccaagcaaa aauuguuuuc uuaccucaac cuguuagacc aucugacuga uaaugagauc     1440

cuaaacauuc ucggguauag ccccgacaag uggaaaauca auuuccggga aauuaucgga     1500

aacagaacaa auaacucaau gaugaaggca uuuuucaaua ugaaagagau gaccgauggc     1560

gaacagauaa acugggagaa gaaucugacg aaaaucacca acggccagag ucuuauuucc     1620

ccugaacaga ucgucgacuu gauuaggcaa uuuuucgagc gcgaggggau cacaacuagc     1680

cugcucuacu uugauccaac cacagacaau uuccgagaac agucucacua uucacugugg     1740

caccuucugu acaguuacga ggaugacaac agccccacug gaaccacauc cuugugcaag     1800

acccucgcua agcgguaugg uauaccucau gaacuguguc cauuacuggc cucucuaacu     1860

uuugagcccc aguacggcuc gcucagcacg aaagcuaugc guaagauucu gccguucaug     1920

caagaaaacg gguaugacaa agccugcgag cuugcaggau acagacacuc cgccuggaca     1980

accaaggagg aaaauacuga gaggaccuug aaaaacucac ugccucuccu gcgcaagggc     2040

aguuuacgga aucccguugu ggaaaagauc cugaaccaga ugacacaugu aauuaauacc     2100

cacaucucug aguaugguaa cccagaugaa auaagaaucg agauggcuag ggacaugcag     2160

gcgaauagca aacagcgaaa ggcaaugacu acagccauug cugaggccac gaaacgcaac     2220

acccaauuua ucgaagagau ucagaaggaa uuuggguuca auccuuccaa aaacgaucuu     2280

aucaaguaca aguuguggaa agagcucgca ccccugggcu caaagacucu auauagcaau     2340

acauacauac caaaagagga acuguuuacc gccaaguucg agauugaaca caucaucccc     2400

cgggcucucu uuuacgacga uucuuucucc aacaaaacac uggagcuuaa uagugugaac     2460

aaguugaagg gaaauaaaac ugcccuggac uauauggaua ccuuaagcca ggaacaggca     2520

aacgacuaca uuaucagagu cgaggagcuc aagaaaauug gcgcgauaac gcaagcuaag     2580

uauaaaaagc ugcuuaucag ggaaaaagau cugggggacg auuuuaccaa ccgugacuug     2640

acaaauacuc gcuacauuau ccggcaugcc gcagauaugc uccuucaggu gaccccuucu     2700

auuguucaca caaacggauc caucacugcc auacuaagaa gggacuggga gcugcucgac     2760

auccugaagg aacuuaauuu gccaaccuau gagucaauug aucgaaagau cagugacccg     2820

aaagaaaagc ugacggagag cguggagcgg aaagaugguc aggcuauuaa gaugaucaaa     2880

gacuggucga agcgcgauga ccauagacac caugccaugg augcauuaau aacagcucuc     2940

accacuagga agcacgucca guaccugaac ggcaaucggg gagccgaaga gacagacaaa     3000

gugcaaaagc ugcagcagcu gaaagcgcua cgaaagcaac ucauuggauc cgauggccac     3060

uucauucccc cuaugcauga caucagagua caggccaaag aagcucuguc ucgcguuauc     3120

auaagcacca aggcaaaggg uaaagugacu accccaaaca caaauaucau uuguucaagg     3180

gauaagacga ccgggcguaa aaucuauguc aagcagacua cauugacacc ccggggcgcc     3240

cugcacaaag agaugcagua cggaugcaac cauuacuauu uagaaaagca aauuccuauc     3300

ggcaguaagc ucaccgagga ggaaauaaga cugguguccu cuccaaaaau caggagcaug     3360

cuuuccgagc gccugcaggc uuacaauaac gaccccaagu cagccuuuac ugggcgaaau     3420

agcauggaua aaaagccguu ggaagacaaa aacaagaagc ucgucuauac cgacaaaaag     3480

gugaaacaga cgauucuggc aaauagauuc guuaagcggg auagggugaa caguaaacua     3540

gaccuggaua agguaaucga cacaggaauu aaacgcaucc uugagcguag acucaagcag     3600

uacgguggca aguaugagac cgcguuuucu gaucuggaaa auaacccuau augguugaac     3660

gaggaaaaag ggauugccau caaguccguc cggaucaggg ggaaaaauaa cacuauuacc     3720

auccgccaaa caaauacuug ucgaauaaag uacguggcua acgacaauaa ccaccaccug     3780

gccguuuaug cauuaccaga uggcagcauu gaggacgaua ucgugucguu cuuugacgcu     3840

gucgaaagau cacggaaagg ucuccccauu aucgauaagc auaagaauca gucucugggg     3900

uggaaauucc uuuuuagugu gaagcagaac gaguacuucc uguugccuca gcaaggcgag     3960

aaaauagaca uugacaccgg agaaaucucc augcagacaa cguucagccc caaggaucuc     4020

ucuccagagu acaucaugaa uccugacaac uaugccgaac uggcacccca cauuuuuagg     4080

guacagagca ucuccaaaac cccacagggc aaucgcgaug ugagaauuua ccuauucaag     4140

caacaguaua uaucagggag ucagaaggac agccugcauc uuaaaggaau cacucacagg     4200

caaauuucuu ccgccaagga ucucgcuggc cugguuaaaa uccgggucaa ccauuugggu     4260

cguaucgugg cgguggggga gaaguga                                         4287


<210>  3342
<211>  4287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3342
augaagaaaa uccugggccu cgaccuuggg accgccagca uuggauccgc uuacgugcug       60

gaggcagaaa agucugccga ucagacaaaa auagucuauu uggguacuag aaucauuuua      120

ucaagugcgg acgcugagaa cuucagcaag ggccuguccu gcucucccac gaccgauagg      180

acauuuaaac ggucgaugcg ccgacgucua caaagauaca agcagaggcg gucaagccuc      240

auccagcagu ucaagaaaau ugggaucaua acuaauaccg aacaaaucau ucgcgagccu      300

aucagugaaa cacacgagcu gcuuagaauu agguccaagg ccguuucuga gcgggugagc      360

cuggaagagu uugcacgcau cuugcucacc auaaacaaac gaagaggaua uaaguccaau      420

aggaaaacuu caaagaguac agaagaggac ggcaagcugg uagaugccau ggcugugacc      480

aaagagcuga uuaagcucaa aaagacgcca ggucaguaca uguucgaacg guuuaaacgu      540

gggaaguaug cagagaucga cuucuacccg uuugauuuaa uuaacgaacu gaaacagauc      600

ugggaggucc agggacaaaa guaucccgac gugcuuacug aagauuugcu gagcucucua      660

cucaagcaga aaaccggcaa ggccaaagag cugaucuacu ccaagucaga ccuuaauccu      720

gcugagguuc caauugauua ccagaaaggc aagaagcugc gcaaaacaau auugaacaag      780

uauuucgccu acgaaagcag aagucucgcg gugacuaaua ucauucccgu cgagcaggca      840

aucaccgcca uaguggcuau uuguaaacaa aucaacaauu cuuccgggua ucugggagcc      900

aucggugaca ggagccgggu auuauacgca aacaaucaga caauuggcca guaucuguac      960

gaacgaauca aggcuaaccc ucaugaucgc auuagagggg agguuuucua uaggcaagac     1020

uaccucgacg aauuugaucg gauaugggag ugccagcaga auuacuaucc agccauccuu     1080

accaacgacc gcaaacagua cauuagaaau aggaucaucu uuuaucaacg uccccugaag     1140

ucgaagaaau cugaguuggc guucugugaa uuugaguccc gggaagugga gcacaacgga     1200

aagaaauaca cggaaggccu acaugucgcc ccuaagucaa guccaguguu ccagcacuuc     1260

cgacuguggc agagacucgg ucagauugag gugagcccgc ugaggaaaug ggauaagcaa     1320

uccaacgagc uuuuugaaca gcagaauaac gcagaccgcg aggggauacu gaauacugaa     1380

gcuaagcaga aauuguucuc uuaucucaac cuguuagauc aucugacaga caaugagauc     1440

cuaaacauuc ucggauacag ccccgauaag uggaaaauca auuuucggga gauuaucggc     1500

aacagaacca auaaccugau gaugaaggcc uucuuuucaa ugaaagaaau gacugacggc     1560

gagcaaauag auugggaaaa gaaucuuaca aaaaucacca acgggcagag uuugauuucc     1620

ccugagcaga ucgucgaccu gauuaggcag uucuuugaac gcgagggaau cacgaccagc     1680

cuccuguauu ucgauccagc uacagacaau uuucgagagc aaucucacua cucacuuugg     1740

caccuguugu acaguuauga agacgauaac agccccacug guaccacauc ccucugcaag     1800

acucuggcca agcgguacgg cauaccucau gaguuauguc cacugcuagc aucucucacc     1860

uucgaacguc aguauggguc gcugagcaca aaagccauga gaaagcuguu gcccuuuaug     1920

caggagaaaa gguacgacga ggcuugcgaa cuggcgggau aucgccacuc cgcauggauu     1980

accaaggagg aaaaugccaa acggacucuc aagaacaagc ugccguuacu gagaaaaggc     2040

ucacuuagga acccuguugu ggagaagauc uugaaucaaa ugacgcaugu aauuaacaca     2100

cacaucgcug aauacgguaa ucccgaugag auacgaaucg aaauggcccg cgacaugcag     2160

gcaaacagua aacagcggaa ggccaugacc gcugccauua cugaggcaac caaaagaaau     2220

acacaguuca ucgaggaaau ucaaaaggag uuuggguuca acccaucuaa gaaugaucuc     2280

aucaaauaua agcuguggaa agaacuagcg ccucugggca acaagacccu cuacagcaau     2340

auauacauuc ccaaagagga gcuguucacu gcuaaguuug aaaucgagca caucauucca     2400

agggcccuuu ucuaugacga uuccuuuuca aacaagacau uggaacugaa uagcgugaac     2460

aaauuaaagg gaaauaaaac ggcacucgac uaccuggaua cccuuggcca ggagaaggcc     2520

aacgacuaua uccugcgugu cgaagaguug aaaaagauug gggcuauaac ucaggccaaa     2580

uacaagaagc uccugauccg cgagaaagau cuaggagacg auuucacaaa ucgggaccug     2640

accaacacaa gauauauuau caggcaugca gcugacaugc uccuucaggu gacucccucu     2700

auuguucaca ccaacgguuc caucacggcc auacuucgac gggauugggg cuugcuggac     2760

aucuuaaagg aacucaaucu gccuaccuac gagaguauug aucgcaaaau cagcgaccca     2820

gaagagaaac uuacacaauc uguggauaga aaggacgggc aggcgauuaa gaugaucaag     2880

gauuggucca aaagggacga ucaucggcac caugccaugg acgcucugau aacugcacua     2940

accacacgaa agcacgucca guaucucaac ggaaauucag gcgccgaaga gacugauaaa     3000

gcucagaagc uggagcaauu gaaagcccug agaaagcagu uaauugguag ugacgggcac     3060

uuuaucccgc ccaugcauga uauccgcgug caggcaaagg aagcgcucag caggguaauu     3120

auaucgacca aaguuaaggg caaagugacg acaccuaaca ccaauaucau uuguucccgu     3180

gacaagacua caggacggaa aaucuacguc agacaaacca cucugacccc aaggggcgcc     3240

cuucaccugg acacaaagua cgggauuaac cgcuauuacc aggagcgaga agugucuauc     3300

ggaagcaagu ugacgcagga ggaaauaaga cucgucucaa guccccauau ccaguccauu     3360

cugcuagagc ggcugagggc uuucgauaau aacccuaaau cugccuuuac cggucgcaau     3420

agcguggaca agaaaccacu ugaggauaag aacaaaaagc ucguuuauac ugacaagaaa     3480

gugaagcaaa caauccuggc aaaucguuuc guaaaaagag aucaggucaa cuccaaguug     3540

aaucuggaca aagugauuga uaccggcauc aagcggauau uagaagggag gcuccagcag     3600

uacggaggca aauaugagac agcuuuuuca gaccuggaaa acaaucccau uuggcuuaac     3660

gaggagaagg guaucgccau caagagcguu cgcauucgag ggaaaaacaa ugcaaucccg     3720

auacaccaaa cugauaccug cccuauugac uacguggcca acgauaauaa ccaccaucug     3780

gcugucuaug uguugccaga cggcacgauc gaagacgaua uuguaacauu cuuugacgcg     3840

guggagagaa gucggcaggg acuccccauc guugauaaga auaaaaacca gucucugggc     3900

uggaaguucc uauuuuccau aaaacagaau gaauacuucc ugcuuccuca acaggccgag     3960

aagauugaca ucgauaccgg ggaaaucagc acucagacca cauucucgcc caaggaccuc     4020

ucaccagagu acauuaugaa cccugauaau uaugcagagc uggcuucuca caucuuuagg     4080

guccagagua uuuccaaaac uccccaagga aaccgcgacg ugagaauaua cuuguucaag     4140

cagcaguaua ucagcggcuc ucaaaaagau agcuuacauc ugaaggguau uaccuacagg     4200

cagaucuccu cagccaaaga ccucgccgaa cuggugaaga uccgggucaa ucaccuuggg     4260

cguauuguua gugugggaga gaaguga                                         4287


<210>  3343
<211>  4287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3343
augaccaaga aaauccuggg ccucgaccuu gggacaacua gcauuggaug ggccuacgug       60

auagaguccg auaaccccga cgaaucugag aucaagcaga uuggugucag aguuaauccu      120

cugacgaccg augaacaaac aaacuucgag aaaggcaagc cauuuucaau caauaaagaa      180

aggacuuuga agcgcggggc ucggcgaaac uuagaccguu auaaggauag aaggaauaac      240

cugauugagg ugcucaaaaa ggcaaauauc cuaagucagg acagcauacu gaccgaggaa      300

ggacagcaca caacccauca gacuuggcaa cuucgggcca aagcggcugu agauaagauc      360

gagaaagagg aacuggcccg cguguugcuc gcaauuaaca agaagagagg cuacaaaucc      420

ucuaggaagg ccaaaaauga ggacgaaggu caggcuaucg augggauggc agucgccaag      480

cggcuguaug acgagcagau uacaugcgga cagcuggugu accaacucuu acgcgaaggc      540

aaaaagucga ucccggauuu cuaucgauca gaccugcagg cugaguuuga uaaaguuugg      600

aacuuccaga agcaguuuua ccccaagaua cuuaccgacg aauuuuauaa agaguugaag      660

ggcaaagggc aaagagccac gagcgcgcug uuuuacaaga cuuacaauuu caacaccgca      720

gagaauaaag gaaguaggga agagaagaag auucaggccu auaaauggcg guccgaagcu      780

aucucucagc aacuagagaa ggaagaggug gccuuugucc ucacagagau uaacaauaac      840

cugaacaaua gcuccgguua ccuuggcgca aucucagacc guaguaaaga acuguauuuc      900

aacaagcaga cuguggggca guacuuguau cagcaacucc aggagaaucg ccacgcuaga      960

cugaaaaacc agguauuuua caggcaggau uauuuagacg aguucgaagc caucuggaag     1020

gaacaagcga agcaucacac cgagcugaca gaggaacuca aagccgagau ucgggauaua     1080

aucauuuucu accagcgaaa acuuaaaagc cagaagggac ugaucucuuu cugugaguuc     1140

gaaucccgcg agauacagau ugacggcaaa aagaagacca ucgguuugag aguugcaccu     1200

aaaucaagcc cacuauuuca agaguucaag aucuggcaga aucugaacaa ugugcucguc     1260

aggaaaaagg ggagucggaa acgcaacgug aagcaggaaa uucagggauc ucuguuugaa     1320

gaggaaaaag agaucuucgu guuugaucuu gagacgaagg aaaagcuguu cgaggaauug     1380

aaucucaaag gcaaccuguc cgcuaauaag gucauugagu uacugggcca uaaacccacu     1440

gaaugggaga uaaacuacag cgcccuagag gggaauagaa caaaccaagc ucucuauaau     1500

gccuaccuga agauccuuga cauugaagga uaugaugagg acuugcugaa acucucggau     1560

aaggacgaua ucaacguugc agaccugaag accccugccu cugaaaucaa aaacauggug     1620

agggacauuu uucagguacu ugguauaucc acugauaucc uggaguucga cgcugaauug     1680

gauggcaagg acuuugagca gcaagcguca uaccagcucu ggcaccuguu auauaguuac     1740

gagggggaug acagccaguc cggaaaugaa aaacuguaug agcuacucga aaagaaauuc     1800

ggcuuuaaga aggagcacuc ucaggugcug gcaaacguca cacuuagcga ugacuacggu     1860

aauuugucaa guaaagccau gcguaagauu uacccauuca ucaaagagaa caauuauucc     1920

accgcuugcg aacuggccgg guaccggcau agcaagcacu cucucacgaa agaggaaauu     1980

gagaaccgac cccuggauga caaguuagaa cugcuuaaga aaaauucauu gagacaaccu     2040

gugguugaga agauccucaa ccagauggug aaugucguga acagucugcu agagaaaaau     2100

aggaaggaaa acccagauuu ucgcuucgac gagauacgga ucgaacuggc aagagagcuc     2160

aaaaagaaug ccaaagaaag ggcugagaug accacaaaca uuaauagcgc caagacugag     2220

aacgaaaaga ucuucaaacu gcuucaggca gaguuuggcu ugaagaaccc gacccgcaau     2280

gauauuaucc gauaucggcu guacgaagag uuaaagaaca auggauauaa ggaccucuac     2340

acaaacaaau auauaccccg ugaaauucug uucuccaagc agguagacau cgagcauauu     2400

aucccucaau cuagacuuuu ugaugacucg uucagcaaua agacuauagu uuacagggag     2460

accaaccugg auaaaggcaa ucagacagcg uuugacuaca ucgauuccaa guaugggaac     2520

gaaaaagugg aggaauacau uuugcgcauc gaggcucucc acgacgccug gaagaaaaau     2580

aaggaggaag gaauuucaaa ggcaaaauau cagaagcugc ugaaaaagga gaccgaaauc     2640

ggugauggcu ucauagagcg ggaccuaaga gaaagucagu acauugccaa aaaggcuaag     2700

gagaugcucu uuaaaaucac uaggucuguc gugagcacgu ccgggucaau cacagauaag     2760

cugcgagagg acuggggacu uauuaacauc augaaagaau ugaaucugcc aaaguaucgc     2820

agcuuaggcc ucaccgagau gcaagaacgg aaauucgguc agaaggucga ggugauuaac     2880

gauuggacua agagaaauga ccacaggcau cacgccaugg augcacugac cguugcuuuu     2940

acaaaacaua accacauaca guaccuuaau uaucugaacg cccguaagaa cgaaaaucau     3000

aaacuacaca acaauaucau ugcgaucgag aaccucgaga ccgaaacuau gauugacaau     3060

aacgggucua agcgccgggu guucaaagcc cccaugccua auuuuagaca aguagcuaag     3120

gagcaccugg aaaacgucuu gaucucccau aaagcaaaga auaagguggu uacgaaaaac     3180

aagaauaaaa uagccggcaa gguggcugcc caggagacac ugaccccaag gggacaguua     3240

cacaaagaaa cugucuacgg caaguaccag uauuacguga acaaggagga gaaaaucggg     3300

acaaaguucg acgaagagac cauuaauaaa guaaguaacc cccaauaucg aaagcuccug     3360

cuugaacggc ugagcgcaaa uggaaacgau ccgaaaaagg cguuuucugg uaagaaugcc     3420

uuguccaaaa acccuaucua ccucaacgag acaaagacug agaccgugcc cgaaguuguc     3480

aaacugacgu ggcuagagga agacuauuca auucgcaagg auaucacccc agacaauuuc     3540

aaagaugaga agaguauaga caagauucug gaugaaggcg ugaaaagaau ccuuaagaaa     3600

aggcuccgga agaacgacaa ugauccuaaa aaggcuuuca gcgaccugga uaaaaacccc     3660

aucugguuga augaggagaa ggggauuucg auaaagcgag ugacaaucuc cggagucaac     3720

aaugccgaac cacugcauua caaaaaggac cacuuuggca acgacauuuu agaugagaaa     3780

ggugacaaga uccccguuga uuucgugucu acugggaaua accaccaugu ggcaguauau     3840

agagacgaaa aaggcaaucu ccaggagaac auugucagcu uuuucgaagc ugugauccgc     3900

guuaaucagg gacugccuau aaucgauaag gccuacaacc agcaccuugg cuggcaauuu     3960

cuguucacca ugaagcagaa ugagauguuu guguucccaa acgagaaauc aggguuuaac     4020

ccgcaggaaa uugacuugcu cgaucccagu aauaagaaaa agaucucccc uaaccuguuc     4080

aggguccaga aaaugucuaa ggugauuuac ggaaauagcc caguucguga guauguguuu     4140

cggcaucacc uagaaacaaa cguagacgag aagaaagaac ugaaggauau cacuuucaaa     4200

aauauaaagu cccuuggcua cuucgaggac auugucaaag ugagaaucaa ccaucucggu     4260

gauaucgugu cagucgggga guauuga                                         4287


<210>  3344
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3344
augaagcaca uccugggccu cgaccuuggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augauaacga acaauccgag auaguugacg uggguguaag agugaauccc      120

cugacaacug augaaaagac gaacuucgag aaaggcaagc cuaucucuac caaugcagac      180

aggacauuga aacggucagc ccgccgaaac uuacagcguu acaagcugag aaggaaggaa      240

cuaauugagc uccugaaaga gcaucagcuu aucaguacug auaccauucu gacagaaaau      300

gggaagggaa ccacuuuuca gacauuggag cuccgggcga aaagcgcuaa ggaaaagauc      360

ucccuggagg agcuggcccg cgucuuccuc accauaaaca agaagagagg cuauaaaucu      420

ucgaggaagg ccaaaaauga agacgagggu caauuaaucg augggaugga aauugcaaag      480

cggcuguacg aggaaaaccu uacgacugga caguugucac ugcagcuacu caaagagggc      540

aagaaaagcc ugccagacua uuaccgcagu gaucuucagu uugagcugga caaggugugg      600

aauuuccaaa agcaguuuca cuccgauauc uugaccgacg aguucuaugc ucagcucaaa      660

ggcaaggggc agcgagcaac aucugcccug uuuuuagcua aauaccaaau uuauacugcc      720

gagaacaagg gaagcagaga agauaaaaag cugcaggcgu accaguggag gguugacgca      780

cucuccaagg agcuuacccg ggaacaacug gccuucguga ucacagagau aaauaacaau      840

uugaacaacu caagugguua ccuaggcgcu auuagcgacc guucuaaaga gcuguauuuu      900

aauaacgaaa ccgucgggga guaccucugg aagcagaucg ccgcaaaucc gcauacgucc      960

cugaaaaacc agguguucua ucgccaggau uaccuugacg aauuugagca aauuuggaag     1020

acucagucag cuuucuaucc cgaaauccug acagaugagu ugaaaaagga gcucagagac     1080

guaguuaucu uuuaccagag gaagcugaaa agccagaaga guuuaguguc uuucugcgag     1140

uucgaaaaua aagaaauuga gauaaagaaa gaggauggaa ccuccacuaa gaaggucauc     1200

ggccugaaag uggccccuaa gagcucgcca cuauuucaag aguucaaaau uuggcagaac     1260

cucaauaacg ugcugauccg gaagaaaggu ucuaagacca aaaaggucgc gaagaaucag     1320

caggccguuc uuuuugaaga agagaaaacc auauccgaau uggaccugga ggcaaagcaa     1380

gcucucuuca acgagcugaa ucuuaaaggg aaccugaagg ccgauuauug uuuggaaauu     1440

cucggauacu cacccaaaga gugggaaaug aauuacagug ugcuggaggg caaccgaacg     1500

aauaaggcuu uauaugaagc cuaccugaag aucaccgacc uagagggcua ugaugccaaa     1560

aaccaccuca agguaaaacu gaacaaggac gagguggaac uugaugacuu gaaagucccu     1620

gcaagcgaga ucaaggacau ggugcaggcu auuuuuaagg aagcggggau caauacagau     1680

auucugcauu ucaacgcaga gcucgacgga aaagauuuug aaaaucaggc cuccuaccag     1740

cuguggcacu uacuguauuc uuacgagggu gacgauagca agucaggcaa cgagcuuuug     1800

uauaaacucc uggaaacuaa guucggguuu aaaaaggagu acgcucaaau acuagccaau     1860

guuagucugu ccgacgauua cggaagccuc ucuaccaagg caaugcgcaa aauccaccca     1920

uauauuaagg aaaacaaauu cucaacagcc ugcgagcugg cuggcuacag acauaguaag     1980

cacagccuua cuaaagagga aaaugacaac aggguguuga aggauucccu ggaggucuua     2040

aagaaaaauu cucuccggca gcccguggua gaaaagaucc ugaaccagau gaucaaucuu     2100

guuaacgccc uguugaaaaa guaucgcacc gagaauccua acuuuaaauu cgacgaaauu     2160

agaauagagc ucgcaaggga gcugaagaaa aaugcggagg aacgugcuaa caugacagcc     2220

aacaucaaua aggcaaagac cgaacaugag aaaauuauca agauucugaa aaacgaauuu     2280

ggucuaccaa auccgacucg gaacgauauc auacgauaca gacucuauga ggagcugaag     2340

gccaaugggu acaaagaccu uuauacgaac aucaagauuc cccaagaaaa guuguucaca     2400

aaagagaucg auauugaaca caucauaccu aagucgaggg uguucgacga cagcuuuucc     2460

aauaaaaccc ugucauuccg caagacuaac uuagauaaag gcgagcggac cgcuuuugac     2520

uacauggaaa guaaguucgg agccgauaag cucgaggagu acgucgcaag aguggaagac     2580

cuguauaaaa auaacauuau cucuaaggcu aaauaccaga aacuucugaa aaaggagagc     2640

gaaauuggcg auggguuugu cgagagggac cuacgcgaga cacaguauau cgccaagaaa     2700

gcgaaggaaa uacuccugca gaucacccca aauguguugu ccacuucagg agguauuaca     2760

gaucgacugc gggaggacug ggauuuaauc aacguuauga aagaacucaa ucugcccaag     2820

uaccgugccc uuggccugac gcaaauggag gacagaaaau augggcaaca gguggaagua     2880

auuaccgauu ggacuaagag gaacgaccac cgccaucacg cuauggaugc auugacaguc     2940

gccuucacca aacauaauca cauccaguac cucaacuauc ugaacgcucg gaagaaugag     3000

aagcauaaag agcacagcaa cauaaugggc auucagcagc uagaaacaau caaggugacu     3060

gacaaaaaug gaaacgagaa gagaguuuuu aaagccccua ugccaaauuu ccgacaggug     3120

accaaggcau uucuggaaaa cauccuuauu ucucacaagg cgaaaaauaa ggucgugacg     3180

aaaaacaaga auaaaguagc cgguuccaac aaggugcagg agguucucac acccaggggg     3240

caacugcaua aggaaacagu cuacggcaaa uaccaguauu acgugcagaa ggaggagaaa     3300

aucggagcua aguucgacuu ggaaacuauu aaaaaggugg ccaauccgcu guaucaaagu     3360

uuacucaaga aacgccugga ggaaaacggc ggggauccua agaaagcauu uaccggaaag     3420

aauagccuug cuaaaaaccc cauauaccug gacgauacac agacugagca guugccagaa     3480

aaggucaaac ucguuuggcu ggaaaauucu uucuccaucc ggaaggacau uaccccugag     3540

aacuuuaagg augaaaaacu aaucgacaag auucuggaug agggugugaa aagaauccuu     3600

aaggaaaggc uccagucauu cggcaaugac gccaaaaagg cauucaguga ucuggacaag     3660

aaccccauau gguugaacaa agagaagggg aucacgauua aacgugugac aaucagcgga     3720

guagccaaug cugagccauu acacaccaag aaagaucauc ugggcaagga aauucucgac     3780

aaggagggua aacccauccc ugucgacuuu auaucgacug gguccaacca ccacguggcg     3840

guuuaucgcg augaaaaggg caaucugcaa gagaaagugg ucucuguguu cgaagccguu     3900

gugcgggcaa accagggacu uccaauuauc gacaagacau acaaucagca ucugggcugg     3960

gaguuuuugu ucaccaugaa acagaacgag auguuuguau ucccgaauga aagcacuggg     4020

uuuaacccca augauaucga ccuacuggau ccuaagaaca agagaauuau aucaccaaau     4080

cucuucaggg uccaaaaauu uaguaagcug caguauggaa acuccuucgu gcgagaguuu     4140

guguuccggc accaucuuga aaccacaguc gaggaccgca aagaauuaca gggcaucacg     4200

uacaagcaga ugaaaaauuc uaccuucuuu aacgauauua ucaagguuag aauuaaucac     4260

cugggugaca ucgugagcau aggggaguac uga                                  4293


<210>  3345
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3345
augaagcaca uccugggccu cgaccuuggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augauaacga acaauccgag auaguugacg uggguguaag agugaauccc      120

cugacaacug augaaaagac gaacuucgag aaaggcaagc cuaucucuac caaugcagac      180

aggacauuga aacggucagc ccgccgaaac uuacagcguu acaagcugag aaggaaggaa      240

cuaauugagc uccugaaaga gcaucagcuu aucaguacug auaccauucu gacagaaaau      300

gggaagggaa ccacuuuuca gacauuggag cuccgggcga aaagcgcuaa ggaaaagauc      360

ucccuggagg agcuggcccg cgucuuccuc accauaaaca agaagagagg cuauaaaucu      420

ucgaggaagg cuaaaaauga agacgagggu caauuaaucg augggaugga aauugccaag      480

cggcuguacg aggaaaaccu uacgacugga caguugucac ugcagcuacu caaagagggc      540

aagaaaagcc ugccagacua uuaccgcagu gaucuucagu uugagcugga caaggugugg      600

aauuuccaaa agcaguuuca cucccauauc uugaccgaug aguucuaugc ucagcucaaa      660

ggcaaggggc agcgagcaac aucugcccug uuuuuagcua aauaccaaau uuauacugcc      720

gaaaacaagg gaagcagaga agacaaaaag cugcaggcgu accaguggag gguugaugca      780

cucuccaagg agcuuacccg ggaacaacug gccuucguga ucacagagau aaauaacaau      840

uugaacaacu caagugguua ccuaggcgcu auuagcgacc guucuaaaga gcuguauuuu      900

aauaacgaaa ccgucgggga guaccucugg aagcagaucg ccgcaaaucc gcacacgucc      960

cugaaaaacc agguguucua ucgccaggac uaccuugaug aauuugagca aauuuggaag     1020

acucagucag cuuucuaucc cgaaauccug acagacgagu ugaaaaagga gcucagagau     1080

guaguuaucu uuuaccagag gaagcugaaa agccagaaga guuuaguguc uuucugcgag     1140

uucgaaaaua aagaaauuga gauaaagaaa gaggacggaa ccuccacuaa gaaggucauc     1200

ggccugaaag uggccccuaa gagcucgcca cuauuucaag aguucaaaau uuggcagaac     1260

cucaauaacg ugcugauccg gaagaaaggu ucuaagacca aaaaggucga uaagaaucag     1320

caggcgguuc uuuuugaaga agagaaaaca auauucgaau uggaccugga ggccaagcaa     1380

gcacucuuua acgagcugaa ucuuaaaggg aaccugaagg cuuauuacug uuuggaaauu     1440

cucggauacu cccccaaaga gugggaaaug aauuauucag ugcuggaggg caaccgaacg     1500

aauaaggccu uauacgaagc auaucugaag aucaccgauc uagagggcua cgacgccaaa     1560

aaccaucuca agguaaaacu gaacaaggau gagguggaac uugacgauuu gaaagucccu     1620

gcaagugaga ucaaggacau ggugcaggcc auuuucaagg aagcugggau caauacagac     1680

auucugcacu uuaacgcgga gcucgaugga aaagacuucg aaaaucaggc aagcuaucag     1740

cuguggcacu uacuguacuc cuaugagggu gaugacucua agagcggcaa cgagcuuuug     1800

uacaaacucc uggaaacuaa guuuggguuc aaaaaggagu acgcccaaau acuagcuaau     1860

guuucacuga gugaugacua uggaucccuc agcaccaagg ccaugcgcaa aauccaucca     1920

uacauuaagg aaaacaaauu uucuacagca ugcgagcugg ccggcuauag acacucaaag     1980

cauagucuua cuaaagagga aaaugauaac aggguguuga aggacagccu ggaggucuua     2040

aagaaaaauu cccuccggca gcccguggua gaaaagaucc ugaaccagau gaucaaucuu     2100

guuaacgcuc uguugaaaaa guaccgcacc gagaauccua acuucaaauu ugaugaaauu     2160

agaauagagc ucgccaggga gcugaagaaa aaugcagagg aacgugcgaa caugacagug     2220

aacaucaaua aggcuaagac cgaacacgag aaaauuauca agauucugaa aaacgaauuu     2280

ggucugccaa auccgacucg gaacgacauc auacgauaua gacucuacga ggagcugaag     2340

gccaaugggu auaaagaucu uuacacgaac gaauacaucg agaagcaaga cuuguucaca     2400

aagaauauug auaucgaaca cauuaucccc aaaucuaggg ucuucgacga cucguucagc     2460

aacaagaccc uguccuuucg caaaacuaau uuagauaagg gcgagcggac cgcauucgac     2520

uauauagaau caaaauuugg agccgauaag cucgaggagu acguggcuag agucgaagac     2580

cuguauaaga acaauauuau caguaaagcc aaguaccaga aacuucugaa gaaagagucu     2640

gaaauuggcg auggguucgu ggagagggac cuacgcgaga cacaguauau cgcaaagaag     2700

gcuaaagaaa uacuccugca gaucaccccu aacguuuuga gcacuuccgg agguauuaca     2760

gaucgacugc gggaggacug ggauuuaauc aaugugauga aggaacucaa ccugccaaaa     2820

uaccgugccc uuggccugac gcaaauggag gacagaaagu augggcagca gguagaaguc     2880

auuaucgauu ggaccaaaag gaaugaccau cgccaccaug cgauggaugc cuugacugug     2940

gcuuuuacaa agcacaacca uauacaguac cucaacuacc ugaaugcacg gaaaaacgag     3000

aagcacagag agcacucaaa uauuauggga auccaacagc uagaaaccau caagguuaca     3060

gacaaaaacg gcaaugagaa gaggguguuc aaagccccca ugccuaacuu ucgacagguc     3120

acuaaggcuu uccuggaaaa ugugcuuauu agccauaaag ccaagaacaa gguagugacc     3180

aaaaauaaga acaaaguugc agguucuaau aagguccagg aggugcucac gccacgcggg     3240

caacugcaca aagaaaccgu guauggcaag uaccaguauu acguccagaa ggaggagaaa     3300

aucggaggca aguuugacuu ggaaacaauu aaaaagguug cgaacccccu guaucaaucc     3360

uuacucaaaa agcggcugga ggaaaauggg ggagauccga agaaagccuu cacugguaag     3420

aacagucuug cuaaaaaucc uauauaccug gacgaugccc agaccgagca guugcccgaa     3480

aaggugaaac ucguguggcu ggagaacagc uuuucuauca gaaaggacau uacaccagag     3540

aauuucaaag augaaaagcu aaucgacaag auucuggaug agggcguaaa aaggauccuu     3600

aaggaacguc uccaguccuu cgggaacgac gcuaaaaagg cuuuuucaga ucuggacaaa     3660

aacccuauau gguugaauaa ggagaaggga aucgccauua aacgcgucgc aaucaguggc     3720

guggccaacg cugagcccuu acauacuaag aaagaucacc uggguaagga aauucucgac     3780

aaagagggca agagcauccc aguugacuuc auaucgaccg ggaauaacca ccauguggcg     3840

gucuaucggg augaaaaggg aaaucugcaa gagaaagugg uuuccguguu ugaagccgua     3900

gucagagcaa accagggccu ucccauuauc gacaagacgu acaaucagca ccuggggugg     3960

gaguucuugu uuacaaugaa acagaacgag auguucgugu uuucuaauga aagcaccgga     4020

uucaacccua augauaucga ccuacuggau ccaaagaaca aaaagauuau aucaccgaau     4080

cucuuuaggg ugcaaaaguu caguaaacug caguacggca acuccuuugu ccgagaguuc     4140

guuuuccggc aucaccuuga aacuacagug gaggaccgca aggaauuaca ggguaucacc     4200

uauaaacaga ugaaaaauuc ugcauuuuuc aacgauauua ucaaaguaag aauuaaccau     4260

cuagggggaa ucgugagcgu cggcgaguac uga                                  4293


<210>  3346
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3346
augaaggaca uccugggccu cgaucuuggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augacaacga aacauccgag auaguugauc ugggugugag aguaaauccc      120

uugacuacgg acgaaaagac caacuucgag aaaggcaagc cuaucucuac aaaugcagau      180

aggacuuuaa aacggucagc ccgccgaaac cugcaacguu acaagcuaag aaggaaggaa      240

cucauugagc ugcuuaaaga gcaccagcug aucaguaccg acacaauuuu gaccgaaaau      300

gggaagggaa cuagcuuuca gacacucgag cugcgggcga aauccgcuaa ggaaaagauc      360

ucucuggagg agcucgcccg cguguucuua accauaaaca agaagagagg cuauaaaucg      420

ucaaggaagg ccaaaaauga agaugagggu cagcugaucg acgggaugga aauugcaaag      480

cggcuuuacg aggaaaacuu gacgacugga caacugagcc uacagcuccu gaaagagggc      540

aagaaaaguc uuccagauua uuaccgcucc gaccugcagu uugaguugga uaaggucugg      600

aauuuccaga agcaauuuca uucucacauc cucaccgacg aguucuaugc ucagcugaaa      660

ggcaaggggc agcgagcaac aagcgccuua uuucuggcua aauaccagau uuauacugcc      720

gagaacaagg gauccagaga agauaaaaag cuccaagcgu accaguggag gguggacgca      780

cuuucaaagg agcugacccg ggaacaguug gccuucguua ucacagagau aaauaacaau      840

cuaaacaaca guagcgguua ccugggcgcu auuucugacc guuccaaaga gcucuauuuu      900

aauaacgaaa ccguggggga guaccugugg aagcaaaucg ccgcaaaucc gcauacguca      960

cuuaaaaacc aggucuucua ucgccaggau uaccuggacg aauuugagca gauuuggaau     1020

acucaagcuc aguucuaucc ccagaucuug acagaugaac ucaagaaaga gcugagagac     1080

gugguaaucu uuuaccagag gaaguuaaag agccagaaaa gucugguuuc uuucugcgag     1140

uucgaaaaca aggagauuga aaucaaaaag gaggauggaa ccuccacuaa aaaggugaua     1200

ggccuaaagg ucgccccuaa aagcucgcca cucuuucagg aguucaagau uuggcagaau     1260

cugaacaaug ugcuuauccg gaaaaagggu ucuaaaacaa agaaagcggc caagaaccag     1320

caagcagugu uguuugaaga ggaaaagacc auauccgagc uggaccucga agcuaaacag     1380

gcccuguuca augagcuuaa ccugaagggg aauuugaaag cugauuauug ucucgagauu     1440

cugggauacu cacccaagga augggagaug aacuacagug ucuuagaagg caaucgaacg     1500

aacaaagccc uguaugaggc auaccuaaag aucaccgacc ucgaaggcua ugaugccaag     1560

aaccaccuga aaguuaaacu uaauaaagac gagguggagu uggaugaccu gaagguaccu     1620

gcuagcgaaa ucaaagacau ggugcaggcg auuuuuaagg aggcagggau caacacagau     1680

auucuccauu ucaaugccga acuggacgga aaggauuuug agaaccaggc uuccuaccaa     1740

uuauggcauc ugcuuuauuc uuacgaaggu gacgauagca aaucaggcaa ugaguugcuc     1800

uauaagcugc uagagacuaa auucggguuu aagaaagaau acgcccagau acuggcaaac     1860

gucagucucu ccgacgauua cggaagccug ucuaccaagg ccaugcgcaa gauccaccca     1920

uauauuaaag agaauaaguu cucaacagcu ugcgaacuug ccggcuacag acacaguaaa     1980

cacagcuuga cuaaggagga gaacgacaau agggugcuga aagauuccuu agaaguucuc     2040

aagaaaaacu cucugcggca gcccgugguc gagaagaucc uuaaucaaau gaucaaccug     2100

gugaaugcau ugcucaagaa auaucgcacc gaaaacccua auuuuaaguu cgacgagauu     2160

agaauagaac uggcgaggga gcugaaaaag aacgcugagg aacgugccaa caugacagca     2220

aauaucaaca aagccaagac cgagcaugaa aagauuauca aaauucuaaa gaaugaguuu     2280

ggucucccaa acccgacucg gaaugauauc auacgauaca gacuguauga ggaacuuaaa     2340

gcuaacgggu acaaggacuu guauacgaau aucaaaauuc cccaggagaa gcuguucaca     2400

aaggaaaucg auauugagca caucauaccu aaaucgaggg uauucgacga cagcuuuucc     2460

aacaagaccu uaucauuccg caaaacuaau cucgauaagg gcgaacggac cgccuuugac     2520

uacauggaga guaaauucgg agcagauaag cuggaagagu acguugcuag aguggaggac     2580

cuuuauaaga acaauauuau cucuaaagcc aaguaccaga aacugcuaaa gaaagaaagc     2640

gagauuggcg auggguuugu cgaaagggac cuccgcgaga cacaguauau cgcgaagaag     2700

gccaaagaga uacuguugca aaucacccca aacgugcugu ccacuucagg agguauuaca     2760

gaucgauuac gggaagacug ggaucucauc aaugucauga aggagcugaa ccuucccaaa     2820

uaccgugcuc ugggcuugac ggaaauggag gacagaaagu augggcaaca gguggaaguu     2880

auuaucgauu ggaccaaaag gaaugaccac cgccaucacg caauggaugc ccucacugug     2940

gcuuucacaa agcauaacca cauacaguac cugaacuauc uaaaugcccg gaaaaacgag     3000

aagcauaagg agcacagcaa uauuauggga auccaacagc uggaaaccau caaaguaaca     3060

gacaagaacg gcaaugagaa aagagucuuu aaggcaccua ugccaaacuu ccgacaggug     3120

acuaaagcgu uucuugaaaa uauucucauc ucucacaagg ccaagaacaa aguugugacc     3180

aagaauaaaa acaaggucgc ugguuccaau aaagugcagg agguacugac gcccaggggg     3240

caauugcaua aggaaaccgu guacggcaag uaccaguauu acguucagaa agaggagaag     3300

auuggaggca aauucgaccu ggaaacaaua aagaaagucg ccaacccguu auaucaaagu     3360

cuccugaaga agcgccuuga ggaaaauggg ggagauccua aaaaggcauu uacugguaaa     3420

uacagccugg cuaagaaccc caucuauuug gacgaugccc agaccgagca gcucccagaa     3480

aaagugaagc ugguguggcu agaaaauucu uucuccauuc ggaaagacau cacaccugag     3540

aacuuuaagg augaaaagcu gauugacaaa auccuugaug agggcgucaa gagaauacuc     3600

aaagaaaggc ugcagucauu cgggaaugac gcaaagaaag ccuucaguga uuuggacaag     3660

aaccccaucu gguuaaacaa ggagaaagga auuacuauca agcguguuac cauuagcggc     3720

guggcuaaug cggagccacu gcacacgaaa aaggaucauc ucgguaaaga aauccuggac     3780

aaggagggga agcccauacc uguggacuuu auuucgacag gcuccaacca ccacguagcc     3840

gucuaccgcg augaaaaagg aaaucuucaa gagaaggugg uuucuguguu cgaagcaguc     3900

gugcgggcua accagggccu gccaaucauc gacaaaaccu auaaucagca uuuggggugg     3960

gaguuucuau ucacuaugaa gcagaacgag auguuuguuu ucccgaauga aagcacagga     4020

uuuaacccca augauauuga ccugcucgau ccuaaaaaca agagaauaau cucaccaaau     4080

cuguucaggg ugcaaaccau uaguguaguc aaguacggca acgccaucgu gcgagacuuu     4140

aaauuccggc accaucuuga gacuaccgug gaagauaaga aagaguuaca ggguaagaca     4200

uaccagcaga uuaaaucccu gucuccccuc aaugacauca uaaagguccg caucaaccac     4260

uugggggaua uuguuagcgu gggagaauau uga                                  4293


<210>  3347
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3347
augaagcaca uccugggccu cgaccuuggg accaacagca uuggaugggc cuacgugaaa       60

gaggcugaaa augauacaga gacuuccgaa auagucgacc uggguguuag agugaacccc      120

uugacgaccg augagaagac aaauuucgaa aaaggcaagc cuaucucuac uaacgcagac      180

aggaccuuaa aacggucagc ccgccgaaau cugcagcguu auaagcuaag aaggaaggag      240

cucauugagc ugcuuaaaga acaucaacug aucagugaga acacaauuuu gaccgaaaau      300

gggaagggaa cuacauuuca gacccucgag cugcgggcga aaagcgcuaa ggagaaaauc      360

ucccugggcg aacucgcccg cguauucuua acgauaaaca agaagagagg uuacaaaucu      420

ucgaggaagg caaagaauga ggaugaaggg cagcugaucg acggaaugga gauugccaag      480

cggcuuuaug aagagaacuu gacuaccggc cagcugucac uacaacuccu gaaagagggc      540

aagaaaagcc uuccagauua cuaucgcagu gaccugcagu uugaauugga uaaggugugg      600

aauuuccaga agcaguuuca cuccgacauc cucacagaug aguucuacgc ucaacugaaa      660

gggaagggac agcgagcaac uucugccuua uuucuggcua aauaucagau uuacaccgcc      720

gaaaacaagg guagcagaga ggacaaaaag cuccaggcgu accaauggag ggucgaugca      780

cuuuccaagg aacugacacg ggagcaguug gccuucguga ucaccgagau aaauaacaau      840

cuaaacaacu caaguggcua ucugggggcu auuagcgacc guucuaaaga acucuacuuu      900

aauaacgaga cgguuggaga auaucugugg aagcagaucg ccgcaaaucc gcauacuucc      960

cuuaaaaacc aaguguucua ccgccaggac uaucuggaug aguuugaaca gauuuggaau     1020

acacaggcuc aauucuaccc ccagaucuug accgacgagc ucaagaaaga gcugagagau     1080

gucgugaucu uuuaucagag gaaguuaaag ucacagaaaa gccugguaag uuucugcgag     1140

uucgaaaaca aggaaauuga gaucaaaaag gaggacggca cuucuaccaa aaagguuaua     1200

ggucuaaagg uggccccuaa auccagccca cucuuucaag aguucaagau uuggcagaau     1260

cugaacaaug uccuuauccg gaaaaagggg ucgaaaacaa agaaagcggc caagaaccag     1320

caggcagugu uguuugaaga agagaagacg auaucugaac uggaucucga ggcuaaacaa     1380

gcccuguuca augagcuuaa ccugaaggga aauuugaaag cugacuacug ucucgaaauu     1440

cugggcuacu cccccaagga gugggaaaug aacuauucag uguuagaggg caaucgaacc     1500

aacaaagccc uguacgaagc auaucuaaag aucacagauc ucgaggggua cgacgccaag     1560

aaccaccuga aagucaaacu uaauaaagau gagguugaau uggacgaucu gaaggugccu     1620

gcuagugaga ucaaagaaau gguacaggcg auuuuuaagg aggcaggaau caacacugac     1680

auucuccauu ucaaugccga acuggacggu aaggauuuug agaaccaggc uagcuaucag     1740

uuauggcauc ugcuuuacuc cuaugagggc gacgauucua aaagcgggaa ugaauugcuc     1800

uacaagcugc uagagaccaa auucggauuu aagaaagaau acgcccaaau acuggcaaac     1860

gugucacuca gugacgauua uggcaaucug uccacaaagg ccaugcgcaa gaucuaccca     1920

uauauuaaag agaacaaguu cagcacugcu ugcgagcuug ccgguuacag acacucuaaa     1980

cacucauuga ccaaggaaga gaaugacaac aggguccuga aagauaguuu agaagugcuc     2040

aagaaaaaua gccugcggca gcccguugug gagaagaucc uuaaccagau gaucaaucug     2100

gucaacgcau ugcucaagaa auaucgcaca gaaaauccua acuuuaaguu cgacgagauu     2160

agaauagagc uggcgaggga acuaaagaag aacgcugagg aacgugccaa uaugaccgca     2220

aacaucaaua aagccaagac ugagcacgaa aagauuauca aaauucugaa gaacgaguuu     2280

gggcucccaa auccgacgcg gaacgauauc auacgauaca gacuguauga ggaacuuaaa     2340

gcuaauggcu acaaggacuu guacacaaac aucaaaauuc cccaagagaa gcuguucacc     2400

aaggaaaucg auauugagca uaucauaccu aaauccaggg uguucgacga uucuuuuucg     2460

aauaagacuc ucagcuuccg caaaaccaac uuagacaagg gagaacggac agccuuugac     2520

uauauggagu ccaaauucgg cgcagauaag cuggaggaau acguagcuag aguugaggac     2580

cuuuauaaga auaacauuau cucaaaagcc aaguaccaga aacugcuaaa gaaagaaagu     2640

gagauugggg auggauuugu ggagagggac cuccgcgaaa cccaguauau cgcgaagaag     2700

gccaaagaga uacuguugca gaucacucga aauguccugu cuacaagcgg uggcauuacg     2760

gaucgguuac gugaagacug ggaucucgug aacgucauga aggagcugaa ucuuccaaaa     2820

uacagagcuc ugggguugac cgaaauggag gacaggaagu auggacaaca gguggagguu     2880

aucauugauu ggacuaaacg caacgaccac cggcaccaug caauggaugc ccucacagug     2940

gcuuucacca agcacaacca uauccaguac cugaauuacc uaaacgccag gaaaaaugaa     3000

aagcacagag agcauuccaa cauaaugggc auucagcaac uggaaacaau caagguaacu     3060

gacaaaaaug guaacgagaa gcgagucuuu aaagcaccca ugccuaauuu ccgccaggug     3120

accaaggcgu uucuugaaaa cguucucauc ucacacaaag ccaagaauaa gguggucacg     3180

aaaaacaaga auaaaguggc ugggagcaac aagguacagg aggugcugac cccacggggc     3240

caguugcaca aagagacagu uuauggaaag uaccaauauu acguccagaa ggaagagaaa     3300

auuggcggga aguucgaucu ggaaacuauc aaaaaggugg ccaaucccuu auaucagucu     3360

cuccugaaaa agagacuuga ggaaaacgga ggugacccga agaaagcauu uaccggcaaa     3420

aauucccugg cuaaaaaccc uauuuacuug gacgaugccc aaacagagca gcuccccgag     3480

aaggugaaac uggucuggcu agaaaauagu uucagcauaa ggaaggacau cacuccagag     3540

aacuuuaaag augaaaagcu gauugacaag auccuugaug aggggguuaa acguauucuc     3600

aaggagcgcc ugcagucuuu cggaaacgac gccaaaaagg cauucuccga uuuggacaaa     3660

aauccuaucu gguuaaacaa ggaaaagggc auaaccauca aacgggugac gauuucaggu     3720

guggcuaaug cggagccccu gcauacaaag aaagaucacc ucgggaagga aauccuggac     3780

aaagagggca agccaauucc cguagauuuu aucaguaccg gaagcaacca ucacgucgcc     3840

guguauagag acgaaaaggg caaucuucag gagaaaguug ugucggucuu cgaggcagug     3900

guuagggcua accaagggcu gccuauaauu gacaagacuu acaaugaaca cuugggaugg     3960

gaguuucuau ucacaaugaa acagaacgaa auguuugugu ucccaaauga guccaccggc     4020

uuuaacccga augauaucga ccugcucgau cccaagaaca aacgaaucau uucuccuaau     4080

cuguuccggg uacagacuau aagcgucgug aaguacggua acgccaucgu gcgcgacuuu     4140

aaguucagac aucaccuuga aaccacaguc gaggauaaaa aggaguuaca ggggaaaacg     4200

uaucaacaga uuaagucacu gaguccacuc aaugacauca uuaaaguuag gaucaaccau     4260

uugggagaua uaguguccgu aggcgaauac uga                                  4293


<210>  3348
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3348
auggcccaga aguacauccu gggccucgac cuugggacca acagcauugg augggcugug       60

gucaaagagg cagaaaauga uaacgagcaa uccgaaauag uugacguggg uguaagagug      120

aauccccuga caacugauga gaagacgaac uucgaaaaag gcaagccuau cucuaccaau      180

gccgacagga cauugaaacg aggggcgcgc cgaaacuuac agcguuauaa gcugagaagg      240

aaggagcuaa uuaaaauccu caaggagcac cagcugauuu cagaugacac uauccuugcu      300

gaaaauggaa aaggcaccac auaccagacc cugaaguugc gggccaaaag uagcaaggag      360

aagauauccc ucgaggaacu ggcacgcguc uuucugacua ucaacaaaaa gagagguuau      420

aaaucuucga ggaagacaaa gaaugaggau gaagggcaac ucauugacgg aauggagauc      480

gccaagcggu uauacgaaga gaaccugacc acgggccagc uuucauugca gcugcuaaaa      540

gaaggcaaaa agagccuccc agauuauuac cgcagugacc uggagcuuga gcuggauaaa      600

guguggaauu uccagaagca auuucauucc gacauuuuga cugaugaguu cuauacccag      660

cucaaaggga agggacagcg agcuacaucu gcacuguuuu uagaaaaaua ccagaucuac      720

acuguugaaa acaaggguaa uagagaggaa aagaaccugc aagccuauca guggagggug      780

gacgcucuca gcaaagagcu uacccgggag gaacuggccu ucgucauaac agagauuaau      840

aacaacuuga auaacuccuc aggcuaccua ggggcgauca gugaccguag caaggaacug      900

uauuuuaaua acgagaccgu gggagaauac cucuggaaac agauugcagc caauccgcac      960

acgucucuga agaaccaagu auucuaucgc caggauuacc uugacgaguu ugagagaauc     1020

uggaauacuc aggcucaguu cuauccccaa auccugacag aauccuugaa aaaggagcuc     1080

agggauguug ugauauuuua ccagcggaag cugaaaucac aaaagagcuu agucaguuuc     1140

ugcgaauuug agaacaaaga gauugaaauc aagaaugagg acggcaccac uaccaaaaag     1200

gugauugguc ugaagguggc cccuaaaucu uccccacuau uccaggaguu caagaucugg     1260

cagaaccuca auaacguccu gauacggaaa aaggggagca aaacagaaaa gguugcuaaa     1320

aaucaacagg ugguacuuuu ugaagaggag aagacgauuu cggaauugga ucuggaggca     1380

aagcaggccc ucuucaacga acugaaucuu aaaggaaacc ugaaggcgga cuacuguuug     1440

gagauccucg gcuauucuuc caaagaaugg gagaugaacu acucagugcu ggagggcaau     1500

cgcaccaaca aggccuuaua ugaagcauac cugaaaauca cagaucuaga gggguaugac     1560

gcuaagaauu accucaaggu caaaaguaac aaggaugaag uggagcugga cgaucuuaaa     1620

guucccgcca gcgaaauuaa ggagauggug caggcuaucu uuaaagaggc cggaauuaau     1680

acugacauau ugcauuucga cgcagaacug gaugguaagg acuuugagaa ccagggcucc     1740

uauaagcucu ggcaccuguu auacucuuac gaaggggaug acagcaaauc aggaaaugag     1800

cugcuuuaua aguugcucga gaccaaauuc ggccugaaga aagaauacgc ccagauccua     1860

gcuaacauua gucuguccga ugacuauggu aaucucagca caaaggcgau gagaaagauc     1920

cauccuuaca ucaaagagaa caaguuuucu acugcaugcg aacuggccgg guauaggcac     1980

ucaaaacaca gucuuaccaa ggaggaaaau gagaaacggg ucuugaagga uggccuggag     2040

gugcucaaaa agaacagcuu acgccagcca guaguugaaa agauucugaa ucaaaugaua     2100

aaccuuguga augcucuguu gaaaaaguac agaacagaga accccaacuu caaauuugac     2160

gaaaucagga uugagcucgc ccgugaacug aagaaaaaug cagaggagcg ggccaacaug     2220

accgcuaaua ucaacaaggc caagacugaa caugagaaaa uugauaagau ccuaaaaucc     2280

gaguucggac ugccuaaucc aacgcgaaac gacauaauca gauauaggcu cuacgaagaa     2340

cugaaggcaa auggcuacaa agaucuuuau acaaaccgcu acauuucuuc ggagaaguug     2400

uuuagcaagg agaucgacau ugaacacauc auaccgaaau cccgggucuu cgaugacuca     2460

uuuaguaaua agaccauugu guauagaacu gacaaccugg auaaagggga gcagaccgcg     2520

uacgacuaua ucgaaucuaa guucggagcu gagaaauuaa aggaguacau gacaaggguc     2580

aaggaucucu aucagaaaaa gauuaucagc aaagccaagu accaaaaacu gcuuaagaag     2640

gaauccgaga uaggugacgg cuucguggaa cgcgaucugc gagagaccca guacaucgcc     2700

aaaaaggcaa aagaaauucu acuccagauc acucauaaug uucugucaac aagcggggga     2760

auuacggacc gguugcguaa ggauuggggc cuggugaacg uaaugaaaga guuaaaucuc     2820

cccaaguaua gagcucuggg ucuuaccaaa auggaggaca ggaaguacgg gcagcaaguc     2880

gaagugauca cugauuggac aaagcgcaac gaccugcggc accacgccau ggaugcauug     2940

accguugcuu uuacaaaaca uaaucacaua caguaucuca acuaccugaa cgccagaaag     3000

aaugagaaac auaaggaaca caacaauauu auggcgaucc agcagcuaga gacuaucaaa     3060

gugaccgacg aaaacggcaa ugagaagagg auuuucaagg ccccuaugcc aaacuuucga     3120

caagucgcua aaacguuccu ggagaaugug cuuaucucuc auaaggccaa aaacaaggua     3180

gugaccaaaa auaagaacaa gauuuccgga aguaauaaag uucaggaagu ccucacaccc     3240

cgcggccagc ugcacaagga gacuguguau gggaaauacc aguauuacgu gcaaaaggaa     3300

gagaaaauag gagcaaaguu ugauagcgaa accaucgaga aggucgcuaa cccuuuguac     3360

cagucucugc ucaaaaagcg guuagaggaa aaugguggcg accccaaaaa ggccuucaca     3420

gggaaaaacu cccuggcaaa gaauccaauu uaucuugacg auacucagac cgagcaacug     3480

ccggaaaaag uuaaguuggu guggcucgag aacucauuua guaucagaaa ggacauuacg     3540

ccugagaauu ucaaagauga aaagcugauc gacaaaauac uagaugaggg agugaagagg     3600

auccugaaag aacgucuuca gagcuuuggc aacgacgcga agaaggccuu cucggaucuc     3660

gagaaaaacc ccauuuggcu gaauaaggaa aaagguaucg cuauuaagcg cguaacaauc     3720

uccggggucg ccaacgcaga gccauugcac accaaaaagg accauuuagg caaggagaua     3780

cuggauaaag aaggaaagcc uauucccgug gacuuuaucu cuacuggcaa uaaccaccau     3840

guugcugugu accgggauga gaaagggaau cuccaggaaa agguccugag cguguucgag     3900

gccguuguga gagcaaacca gggacuucca aucauugaga aaacauauaa ucaacaccug     3960

ggcugggaau uuuuguucuc aaugaagcag aacgagaugu ucguauuucc caaugaaagu     4020

accgguuuca acccuaauga cauagaccua cuggauccaa agaacaaaag gaucauuucc     4080

ccgaaucucu uuaaggucca gaaacuggcc acuaagaacu acauguucac ccaccaucuu     4140

gagacaaaau cuaucgacgg ggauauguua aagaauaaaa aggaacugag cggcauucuc     4200

uauaacuuua uccagacgcc ccaaaaguug uacggaauaa ucaaagugcg aauuaaccac     4260

cugggugaca ucguguccgu cggcgaguau uga                                  4293


<210>  3349
<211>  4296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3349
augaagagaa uccugggccu cgaccuuggg agcuccucua uuggaugggc ccuggugaac       60

gaggcugaau caaagaauga gaggaguagc auagucaagu uggguguucg ggugaacccc      120

uuaaccguag augaauccau caauuucgag aaaggcaagu cuauuacaac uaacgcagac      180

cgcacgcuga aacgaucgau gcguagaaau cuacacaggu acaagcuccg gcgcgaagcc      240

cugaucgagg ugcuuaagga gugcaaauuu auuaccgaag auacaauccu gucagagaac      300

gggaauagaa cuaccuucga aacauauagg uugcggguca aggcgcucac cgaggagaua      360

agccuggaag aguuugcucg cgugcugauc augauuaaca aaaagcgagg auacaaaagu      420

uccagaaagg ccggcggugc agaagaggac acucucaucg augggaugga cauugccagg      480

aaguuauaug augcuaaucu gacaccugga gaacuuuguu ugcagcugcu aucugcaggc      540

aaaaagguuc ucccagacuu cuaccggagc gaucugcaaa acgagcuuga ccguaucugg      600

gagaaacaga aggaauauua cccgggcaua cugaccgccg cuuugaaaga ggaacuccgc      660

gggaagaaaa gagaugccgu gugggcgauu ugcgcaaaug ccuuugucug gaaggagacg      720

uauacugaau ggaacaauga ggaguccaag aacgaacaga ucgagaaaga acauaagcug      780

gagggaauuu acucaaaaag gaagcgggac gaagcuaaaa aggagaauuu acaguggcga      840

gugagugguc ugaaggagaa acucagccuu gaacaacugg uaaucaucuu gcaggagauu      900

aacacccaga uaaacaauuc uuccggcuac cuagggacaa ucucagaucg cagcaaggaa      960

cuguauuuca acaaacagac uguuggacaa uaccucaugg acauucugga caauaacccc     1020

aaugccaguc uuagaaacau cguguuuuau aggcaggauu accuggacga guucaauguc     1080

uugugggaga agcaggcaga auuucacaaa gagcucaccg gcgaacugaa gaaggagaua     1140

cgggauauua ucaucuucua ucagcgcaga uuaaaaucuc aaaagggucu gauuuccuuu     1200

ugugaguucg agagcaggca gaucgaggug aaaauugacg ggaagcguaa aauacggaca     1260

gugggaaacc gagucauccc uagaucgucu ccacuauuuc aggaguucaa gauuuggcaa     1320

auccucaaua acaucgaagu uaccgcugug ggcaauaaga acaaacagag gaugcagaau     1380

ggcuccaacg aacccgauga cacggagcag cuggaacuua augggcgccg guacuuguau     1440

caagaggaga aggaacugcu cgccaaagag cuguucguaa gagauaagau gacuaaacuu     1500

gacgugcuga gguugcucuu ugauaacccu gaagaacugg acuuaaacuu caagacaauu     1560

gauggaaaua aaaccgguua ccuguuuuuc caggcguacu caaagaugau agaaaugagu     1620

ggccaugagc cagucgacuu uaagaaaccc aucgagaaga uuguggaaca gguuaaaagc     1680

guguucgucc uacucaacug gaauacugac auccuguccu uugauucuaa caaggagcuu     1740

gacaaacaga gcuauuacaa guuguggcau cugcucuauu cauucgaagg ggauaauaca     1800

ccuaccggaa acggcugccu ggugcaaaag auuauggaca guuacgguuu agggaaagag     1860

uuugccaaga uccuggcaaa uauaacguuc caggaggauu auggcucccu uagcgcuaaa     1920

gccauccaca agauuuugcc acaccucaaa gaaggaaacc aguacgacgu agcuugugag     1980

uaugccggcu accgccacuc uaagucaagu cugaccaagg aagagaucgc aaauaaaguu     2040

cuaaaggaua aacuggaagu gcucccgcga aacagccugc ggaaucccgu cguggagaag     2100

auucuuaacc agauggucaa ugugaucaac gccauaauug aggcuuacgg gcguccugac     2160

gaaaucagag uugaguuggc gagggaacug aaaaagaaug caaaggagcg cgaaaaauua     2220

acaaagucca uugccgagac uaccaaaaca caugaggcuc acaagaaacu ccugcaagaa     2280

gaguuuggac uuaagaacgu gggucggucu gauauccuga gauauaaauu guacaaggaa     2340

cucgagucga ggggcuauaa gacucuguac agcaacaccu auauaccacg agaaaaacua     2400

uucuccaagg aguuugacau cgagcauauu aucccccagg cccgccuguu cgaugacuca     2460

uucaguaaua aaacacucga agcacggaac guaaauauug agaaggggaa cagaaccgcc     2520

uacgauuuug ucaaagaaaa guucggagag ucuggcacug acaauagccu ggacugcuau     2580

cuuaacaaua ucgagguguu guuuguuucc gguaagauau caaaaacgaa guacacaaaa     2640

cugaagauga ccgaacagga uauuccuggg ggcuucaucg agagggacuu acguaacacu     2700

caguacaucg cuaaaaaggc ccucagcaug cugaaugaaa uuucucgcug uguggucgca     2760

accuccggaa guaucacaga uaaacuucgg gaggacuggc aacuggugga uguaaugaaa     2820

gaacuaaacu gggagaagua uaaagcgcuc ggccuggugg aguacuuuga agacaaggau     2880

gggaaacaga uuggaagaau aaaggacugg agcaagagga augaucaccg acaccaugcu     2940

auggacgccu ugaccguugc auucacuaaa gaggucuuua uccaguauuu caacaauaag     3000

aacgccucuc ugaauccaaa cacaaacgaa uacgcuauua aaaauaagua uuuucaaaac     3060

ggucgggcca ucgcacccau uccguuaaau gaguuccgcg cugaagccaa aaagcaccuc     3120

gagaacacgc ugaucuccau aaaggcgaaa aauaagguga ucaccaacaa uauuaacaaa     3180

acuaagaaaa gaggcggggu gaauacaaag aaacagcaga ccccuagggg acagcuucau     3240

cuggagacaa ucuacggcug cugucggcaa uauuugacua aggaagagaa ggucaacgcc     3300

ucauuugaug uuaguaaaau ugcuaccgug agcucguccg uguaccgaga cgcacuccug     3360

aagagacuac aggaacacca uaaugauccc aaaaaggccu ucgcuggucg caacucucug     3420

gacaaaaagc caguauggcu ugacaaggag cagaucagga aagucccuga aaaggugaaa     3480

acgguuaccu uugagacaau auacacuauu cguaaggaga ucagccccga ucuccgggug     3540

gacaaaauca uugauucagg ggucagaaag augcugaccg gcagguugaa ggaauaugga     3600

aaugacgcca aaaaggcauu caguaaccug gaugagaaac caauaugguu aaauaaggaa     3660

aaaggcaucc ccauuaagcg cgugacaauc uccgggauuu cuaacgcgca gagccuccac     3720

guuaagaaag acaaggaugg aaaaccugug cuggacggca auggucgaaa caucccagua     3780

gauuucguca acacugggaa uaaccaccau guggccgugu acagacggcc ggucauagac     3840

aagaauggac aaaugguuau cgaugagaac ggcaaucuua aauaugagcu ggaagaggug     3900

guagugaccu uuuucgacgc ugucacgagg gugaacuugg gucagcccau uaucgacaag     3960

gauuacaaag ccuccgaagg cuggcaguuu cucuucucaa ugaagcagaa ugaguauuuu     4020

guuuucccua acaagaaaac aggguuuaau ccaaaggaaa uugaccugcu agaugcagac     4080

aacuacggac ugaucagccc caaucuuuuc cgcgugcaaa aauuuaccca caagaacuau     4140

gucuuccguc aucaccucga gacuacaaua aaagauacca guucuauucu gagaggcauc     4200

acuuggaucg acuuucgguc cagcaagggg uuggauucga uugugaaggu uagggugaau     4260

cacaucggac agauagucuc aguaggcgag uacuga                               4296


<210>  3350
<211>  4299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3350
augaagaaaa uccugggccu cgaccuuggg agcaacucca uuggaugggc ccuggugaau       60

gaggcugaaa acagagauga ggcaucuuca auaaucaagu ugggugucag gguuaauccc      120

uuaaccgugg acgaaaugca gaacuucgag aaaggcaaga guauuacaac uaaugccgau      180

cggacgcuga aacgcagcau gcgacguaac cuacaaagau acaagcucag gcgggacgcg      240

cugaucaaac uucugaaaaa gguaggguuu auugcugaug gaaccaucuu guccgaacag      300

ggcaaucgcu cuacauucga gacuuauaga cucagggcca aagcaccuga cgaggaaaua      360

ucgcugcagg agcuggcccg ggugcucuua augaucaaca agaaacgcgg uuacaaguca      420

agccgaaaga uuaaagaaaa ggaggcuggg acccugaucg auggaaugaa agucgcaaag      480

aaacuuuaug acgaggauuu gacaccaggc cagcugugcc uacaacuccu ggaaaguggc      540

aagaaauccc uuccggacuu uuacucuagc gaucugcagg aggaauuuga cagaauuugg      600

aauuuucaga agcaguucaa ccccaaggcc uuuugugaua ccgcuaaaga ggaaaucaag      660

gggaaaaaua agucccaaac uugggccaua uuggcgaaau auuucauuug gacagacgag      720

auggugguuu ggaacgagaa ggaaucaaag accgagaggg uggaaaaaga gugcaagcuc      780

gucggacuga aacggacggu gaagaaagag gaacagcgua aggagaauua ccgcuggaga      840

guacaggcau uaacugaaca gcuggaccuc gguaagguug ccgugguccu ucaagagauc      900

aacggccaga uuaguaccag cucuggguau cugggagcua ucuccgauag gucaaaagug      960

uuguacuuua aucggcagac agugggccaa uaccagaugg ccgaacuaga gaagaacccu     1020

aacacuagcc ugcgaaauau ggucuucuau cgccaggacu accucgauga auuugacaaa     1080

aucugggaga agcaggcaga guuccacaaa gaacugaccg uugagcuuaa gaaggaaauu     1140

agagauauaa ucauuuucua ucaaaggcgg cugaaaaguc agaaggguuu gaucggguuc     1200

ugugaguucg agaaccgcga aauaauggug gagaaaaaug gaaagaugca ggcuaaagua     1260

gugggcugca aggucauucc aagaucucau ccccucuuuc aggaauuuaa aaucuggcaa     1320

acacugaaca auguggaggu uuuugccggc gaaaagaagu guaaaaggaa gcguaccgac     1380

gcguucccug gguuauuuga uggaacggag gacacucuga ugguggaggg uuggcgguac     1440

cuauaucagg aagagaaaga aauccucgcc aaggagcugu ccgucaaaaa gagccuuaag     1500

aaagaauaca uuuugaagcu ccuguucgau aacccacagg accuggauau gaauuuugag     1560

cagaucgagg gcaacgacac agggaaugug cuuuucucgg cauauucuaa aaugauugaa     1620

aaguacggau acgagccccu ggacuuuggc aaauccaccg augaaauaau cgagcgauug     1680

caaguaauuu ucacugaccu cgguuggaac acagaucugu uauuuguuga ccugaauaag     1740

gaagggaagg aucuagagaa acagccuuau uucaagcucu ggcaucugcu uuacucauuu     1800

gagggcgaca acaccccaac gggaaauggc aaauuggugg aaaagcugau ggagcucugc     1860

cacgucggga aagaaugugc ucuggaguua gugaacauca guuuccagaa ugauuaugga     1920

agccuguccg ccaaggcuau caagaaaauu cuuccguacu ugaaggaggg uaacagauau     1980

gacgucgccu gcggcuacgc aggguauagg cacucuaaaa gcucacucac caaggaagag     2040

auagaaaaca aagugcugaa ggauaaacua gacauccugc ccaagaauag ucuccgcaac     2100

ccuguuauug agaagauccu gaaucagaug gugaacguaa uuaaugccau cauagauaca     2160

uacggaaaac cagacgaaau ccgggucgag cuugcuagag aguugaagaa auccgcgaag     2220

gaaagggaug agcugacuaa agcaauugcc aagagcaccc gcgaacauga ggaaauccga     2280

aagguguuac aagaggaguu cggcaugauu aacguuucuc ggaaugacau cauacguuac     2340

aaacucuaug aggaacugaa ggacaacggu uacaaaacac uuuauucaaa ucaguacauu     2400

cccaaggaaa aaguguuuag uaaggagauc gauauugaac acaucauacc ucaggcuaga     2460

cuguucgacg auagcuuguc caacaagacu cucgaguaua aagccaucaa uauugagaag     2520

gggaacaaaa ccgcauacga cuuugucaag gaaaaauaug gcgagaaggg acugcaacag     2580

uaccuaaaua ggugugaauc ucuguucaag ggccaccgca caaaacuccg gaagcugaaa     2640

auggggcagg agaacauccc agagggauuu auugauagag accuuaggaa uacccaguac     2700

aucgccaaga aggcuuuggc caugcugaac gaaauaugcc gacgcguggu agcaacuacg     2760

gguucgauua cagaucgguu aagagaggac uggcaacucg uggauguucu gaaggaacuu     2820

aaccugccca aguaugagac ccuaggccuc gucgaagugu acgaggacaa agaugggagg     2880

aagaucaaac guaucaagga cuggacuaaa cgcaaugauc aucggcacca ugcgauggac     2940

gcucugaccg uggccuucac aaaggacguc uuuauucagu auuucaacaa uaaaaacgca     3000

gccuggcagg cugauuguaa ggagcacagc gacaucauug gaauaaagac cagauacuuu     3060

gaaaauggca gggccuugcc gccuaugccc cugguucgau uccgggcaga ggcuaaaucc     3120

cacuuagaaa cucuccuggu gucaaucaag gccaaaaaca aggugguaac aaauaacguc     3180

aauugcacga aacgcaaggg uggggagaac aagaaacuuc agcaaacccc aagaggccag     3240

cugcauuugg aaacuauuua uggaagucac aggcaguacg ugaugaagga ggagaaaguu     3300

aaugcgucuu uugauagcuc caagaucgcc acagugugca aacaggcuua ucggaacgca     3360

cuccuggaac gacuagccgc uuucggcaau gacccuaaga aggccuuuac cgggaaaaac     3420

ucacuggaga agaaucccgu cuaccuugau agagaacaaa cagcacgcgu gccagagagg     3480

guucguacug uggaacucga gaccguauac acgauucgga aacccgucga cccuaaccug     3540

agcguggaua agguggucga cgcgaaaguu agaucuaucu uggagcagag gcugcgcgaa     3600

uaugauggag aggccaagcg agcuuucgug aacuuagacg aaaauccaau auggcucaac     3660

gaggagaagg gcaucgccau uaaaagagua accauccggg guauuaauaa ggugcagucc     3720

cugcauggga aaaaggauaa aaacggaaau cuuauccugg acgaaacagg caagaaaaua     3780

ccggucgacu ucgugaacac ugguaauaac caccauguug caauuuacag gaagcccgug     3840

uuggauaaga ccggccaaga gaugcucgac aaugaaggga acccugucua ugagcuggau     3900

gaagugguug ugaguuucuu cgaggcuguc acacgcguaa aucuaggacu gccagugauc     3960

gacaaaaacu acaauaagag cgagggcugg caguuuaugu ucacuaugaa acagaacgaa     4020

uauuuuguuu uccccaauga gaagaccggg uuugauccua aagaagugga ccuucucaac     4080

ccagagaauu acgccgucau cucucccaac cuguuccgug ugcagaagau uacggaaaag     4140

gauuauaugu uuagacacca cuuggagaca aaaguagugg acgauaaguu cuuaaaagag     4200

auaaccugga agcggauccu gacuccgaac aaacucgaag gaauugucaa gguuagggug     4260

aaucauaucg gccaaauugu guccgucggu gaguacuga                            4299


<210>  3351
<211>  4299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3351
augaagaaaa uccugggccu cgaccuuggg agcaacucca uuggaugggc ccuggugaau       60

gaggcugaaa acucugauga ggucucaagu auaaucaagu uggguguuag agugaauccc      120

uuaaccguag acgaaaugca gaacuucgag aaaggcaaga gcauuacaac uaaugcagau      180

aggacgcuga aacgguccau gcgccgaaac cuacaacguu acaagcucag aagggacgcc      240

cugaucaaac uucugaaaaa ggugggguuu auuaccgaug gaacaaucuu gucugaacag      300

ggcaaucgga cuaccuucga gacauaucgc cucagagcga aagcuccuga cgaggaaaua      360

ucgcugcagg agcuggccag gguccucuua augaucaaca agaaacgggg uuacaaguca      420

agccgcaaga uuaaagaaaa ggaggcaggg acccugaucg auggaaugga gguggccaaa      480

aagcuguaug acgaagauuu gacuccaggc cagcugugcc uacaacuccu ggagaguggc      540

aaaaaguccc uuccggacuu uuacucuagc gaucugcagg aagaguucga ccgaauuugg      600

aauuuucaga aacaguucaa ccccaaggcu uuuugugaua cagcaaagga agagaucaaa      660

gggaagaaua aaucccaaac cugggccaua uuggcuaagu auuucauuug gacggacgag      720

augguugugu ggaacgaaaa agagucaaag acugaaagag ucgagaagga augcaaacuc      780

gugggacuga agaggaccgu aaagaaggag gagcagcgga aagaaaauua cuguuggcgu      840

guucaggccu uaacagagca gcuggaccuc gaaaaggugg cggucgugcu ucaagagauc      900

aacggucaga uuaguacuag cucuggcuau cugggggcaa ucuccgaucg cucaaaggug      960

uuguacuuua auagacagac cgucggacaa uaccagaugg ccgaacuaga gaaaaacccu     1020

aacacaagcc ugaggaauau gguuuucuau cggcaggacu accucgauga guuugacaag     1080

aucugggaaa aacaggcuga guuccacaag gaacugaccg uggagcuuaa aaaggagauu     1140

cgagauauaa ucauuuucua ucaacgcaga cugaagaguc agaaaggcuu gaucgguuuc     1200

ugcgaauuug agaacaggga aauaauggua gagaagaaug ggaaaaugca ggccaaggug     1260

gucggaugua aagugauucc acggucucau ccccucuuuc aggaguucaa gaucuggcaa     1320

acgcugaaca auguugaagu guuugcaggc gagaaaaagu gcaagcgcaa aagaacugac     1380

gcuuucccug gcuuauuuga ugggacagaa gacacccuga uggucgaggg auggagguac     1440

cuauaucagg aagagaagga aauccucgcc aaagagcugu ccgugaagaa aagccuuaag     1500

aaggaguaca uuuugaaacu ucucuucgau aacccacagg accuggauau gaauuuugaa     1560

cagaucgagg guaacgacac uggcaaugua cuuuucucgg cguauucuaa gaugauugaa     1620

aaauacgggu acgagccccu ggacuuugga aagccuacag auaaaauaau cgaacaauug     1680

cagguuauuu ucgccgaccu cggcuggaac accgaucugu uauuugugga ccugaauaag     1740

gaggguaagg aucuagagaa acagccauau uucaagcucu ggcaucugcu uuacuccuuu     1800

gaaggggaca acacgccgac cggcaaugga aaauuggucg agaagcugau ggaacucugu     1860

cacgugggca aagagugcgc acuggaguua gucaacaucu cauuccagaa ugauuauggg     1920

agucugagcg cuaaggccau caagaaaauu cuucccuacu ugaaggaagg aaaccguuau     1980

gacguggcuu gugguuacgc cggcuaucgg cacuccaaau cuagccucac aaaggaggaa     2040

auagagaaca aaguucugaa ggauaaacua gacauccugc cuaaaaauuc acuccgaaac     2100

ccagugguag aaaagauucu gaaucaaaug gucaacguga ucaaugcaau uaucgauacu     2160

uacgggaaac ccgacgagau aagaguugag cuugccaggg aauugaagaa aagugcuaag     2220

gagcgcgagg aacugaccaa agcgaucgca aaguccacac gggaacauga ggagauuaga     2280

aagguguuac aggaagaguu cggaaugauc aacgucagca ggaaugauau uauccgcuac     2340

aaacucuaug aggaacugaa ggacaacggc uacaaaacuc uuuauucuaa ucaguacaua     2400

ccuaaggaaa aaguguuuuc aaaggagauu gacaucgagc acauuauccc acaagcccga     2460

cuguucgaug acaguuugag caacaagacc cucgaauaua aagcuauaaa uaucgagaag     2520

gguaacaaaa cagccuacga uuuuguaaag gaaaaauaug gggagaaggg ccugcagcag     2580

uaccuaaauc ggugcgaguc ccuguucaag ggacagcgua ccaaacucag aaagcugaaa     2640

augggccaag aaaacauucc cgagggguuu aucgacaggg aucuucgcaa uacucaguac     2700

auugcaaaga aagccuuggc uaugcugaac gaaaucuguc ggagaguggu ugccacgaca     2760

ggaucuauaa ccgacagguu acgagaggau uggcagcucg ucgacgugcu gaaggaacuu     2820

aaccugccga aguaugagac ucuaggucuc guggaggucu acgaagauaa agacggccgc     2880

aagauuaaac ggaucaagga uuggaccaaa agaaaugacc acaggcauca cgcaauggau     2940

gcgcugacag uugcuuucac caaggacgug uuuauccagu auuucaacaa uaaaaacgcc     3000

gcauggcaag ccgacugcaa ggagcauucg gauauuaucg ggauuaagac ucguuacuuu     3060

gaaaauggac gcagcuugcc ucccaugcca cuggugcggu ucagagcuga ggccaaaucc     3120

cacuuagaaa cacuccuggu aucaauaaag gcaaagaaca aggucgugac gaauaacguu     3180

aauugcacca aaaggaaggg cggugagaac aagaaacuuc agcagagucc ucgagggcag     3240

cugcacuugg agacuaucua uggcucucau cggcaauacg ugacaaagga agagaaaguc     3300

aaugcuagcu uugacuccuc aaagauugcc accgugugua aacaggcgua ucgcaacgcc     3360

cuccuggaaa gacuagcugc auucggaaau gaucccaaga aggccuuuac aggcaaaaac     3420

agccuggaga agaauccagu uuaccuuaac agggaacaga cugcucgggu gcccgagcga     3480

guaagaaccg ucgagcucga aacgguguac accauccgca aaccugugga cccaaaccug     3540

ucugucgaua agguugugga cgccaaagua agguccauuu uggagcaacg ucugcgggaa     3600

uaugaugggg aggcaaagag agcguucgug aauuuagacg agaacccgau cuggcucaau     3660

gaagagaagg gaauagccau caaaaggguc acaauucgcg gcaucaacaa ggugcagagu     3720

cugcacggua aaaaggauaa aaaugggaac cuuauucugg acgaaacugg aaagaaaauc     3780

cccguugauu ucgugaauac cggcaacaau caucacgucg cuauauaccg aaagccugug     3840

uugaacaaga caggucagga gaugcucgac aaugaaggca acccaguuua ugagcuggac     3900

gagguggucg ugagcuuuuu cgaagccgua acuagaguua aucuagggcu gcccgugauu     3960

gauaaaaacu acaauaaguc ugagggaugg caguuuaugu ucaccaugaa acaaaacgaa     4020

uauuuugucu ucccuaauga gaagacgcgg uuugacccaa aagaagugga ucuucucaac     4080

cccgagaacu acgcaguaau cuccccgaau cuguucaggg ugcagaagau cacagagaag     4140

gacuauaugu uucgccauca cuuggaaacc aaagucguug augacaaguu cuuaaaagag     4200

auuacuugga agcguauacu gacaccuaac aaacucgaag gcaucgugaa ggugagaguc     4260

aaucacauug ggcagaucgu gucaguugga gaguacuga                            4299


<210>  3352
<211>  4299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3352
augaagaaca uccugggccu cgaccuuggg accacaagca uuggauucgc ccacauagug       60

gaggaugaaa auaaagagaa guccgaaauc aaagagcugg gugucagaau uguuucuuug      120

acuacggacg aacagucaga uuuugagaag ggcaaaagua ucaccacaaa cgcuaauagg      180

acuuuaaagc auggggcacg gcugaaccua gaccgcuacc aacagcgacg uaaguaucuc      240

auugaucugc uucacaaagc caaucugauc acccccagcu ccauauuggc ggagaacgga      300

aagaauacaa cccauucuac uuggcagcuc agagcuaaag ccgugacaga aaggaucgag      360

aaggaagagu ucgcacggau ucugcuggcc aucaacaaaa agcgcggcua caagucguca      420

agaaaagcua agaccgagga cgaaggucag gcaauugaug ggauggccau cgcuaaaagg      480

cucuaugacg agaauuuaac gccuggacaa cugagccuuc aguugcugca gcagaacaag      540

aaacuacucc cagauuuuua ccggagugac cugcaaaagg aauuugaucu uguauggaau      600

uuucagaaac aguucuaucc ggacauacug acugauauuu ucuacaagga guugcagggc      660

aagggcaaag acgccaccuc caaggcguuc ucuaaacgcu aucacuuuga cacagcagaa      720

aacaagggga gcaaagaguc cgugcgacuc caagccuacc aguggagagc ugaggccguc      780

ucaaagcagc ugaguaagga agagguggca uacguuuuaa cugaaaucaa uaacaaucug      840

aacaacgcua gcggauaucu cggugccauu ucugauaggu ccaaagagcu uuacuucaau      900

cggcaaaccg ugggccagua ucuguacgcg caguugcagg aaaaccguca uaauucacua      960

aagaacaaag ucuuuuaucg ccaagacuac cuggaugagu ucgagagaau cugggaaaca     1020

caggcccucu uucacaagga gcugaccgac gaacuuaaaa agcagaucag ggauguggua     1080

auuuucuauc agcggaagcc caaaagccaa aaggggcuga uaaguuucug cgaguuugaa     1140

ucuaaagaga ucgagauuga aaaggacgga aaaacgauca cuaagaauau aggcgcacga     1200

guugugccua aguccagccc auuguuccag gaguuuaaaa uuuggcagau ccucaacaau     1260

gucaucugua agcgcaaagg uauuagaaag aaaaagaucu cggcuaaaac aacccagcug     1320

gauuuacuga acgaaucuuc ccaaacuauu uucucacuag acauggagug caagcagcuc     1380

cuguuugaug agcuuaauuu gaagggggac cugaaaagug auaaggugcu caaacugcuu     1440

ggauacagcc cccaggaaug ggagauaaac uacaaucagc uggaaggcaa caggacacaa     1500

aaggccuugu augaggcuua ccucaaaauc guggaaaugg aggcccacga cgucaaggac     1560

auucugcaga ucaaguccac caaagaugac uggucuuuag augagagccc ucugucagca     1620

agugaaaucc gggaguccgu uaaggccauu uuccaggcuc uaggcauaug uacgaaaauc     1680

cucuauuuug acccacugcu ucccgugaag gauuucgagg aacaagacag cuaccaguug     1740

uggcaucugc ucuauucuua cgaaucagau gacaguacca gcgggaauga gacacuguau     1800

cgcauuuuag agaaaaagua cgcguuuaag agagaacacg caaggauccu ggccaacgua     1860

gcccuucagg augacuacgg auccuugucu acuaaagcua uucguaagau cuauccuaau     1920

auaaaagaga accaguacuc gaccgcaugc gaaauggccg guuauaagca uagcaaacuc     1980

ucccugacaa cugaggaacu agaggcucgg gagcugaaga acaucauucc acuccugaag     2040

aaaaaugccc uucgaaaccc gguggucgaa aagaucuuga aucaaaugau uaacgugguu     2100

aaugcacuga ucgagaaaaa cucagaaaga gaugcggagg gcaagauaac caaauacuuc     2160

cacuuugacg agauuaggau cgaauuagcu cgcgagcuca agaaaaaugc ccagaagcgg     2220

uaugaaauga cacagaacau uaauaaggca aaaagugagc accagaagau cucugaaaua     2280

cugcaaaaag aguucgggau caagaacccc accaaaagcg auauuaucag auacaggcuu     2340

uauaaggagc uggaacauaa uggauacaag gaguuguaca cuaacacgua uauugccccu     2400

gacgugcucu uuuccaaaaa uaucgacaua gaacacauug ucccaaaggc ucgcguguuc     2460

gaugacucau ucagcaacaa aacacuggcc uuuaaggauu ucaauaucaa aaagggcgag     2520

ucuaccgcau uugacuacau cacuucccua aagaacgaag agaaacugaa ccaguaucuc     2580

gcucgaguag agaaugccua caagaccaaa aacauuaguc ccgcgaagua uaaaaaucug     2640

cuuaagaagg ccagcgaaau cggugauggg uucauuaacc gggacuugcg ugauacacag     2700

uacauagcua aaaaggcaaa agagauccug uuucagguua ccaagaaugu guuaucuacu     2760

uccggcucaa uuacagacag acucagggaa gauuggaguc uggucgacgu gaugaaagag     2820

cuuaacaugc cuaaguauca aagccuggga cuaacggaag ucgaggagcg caaggauggc     2880

aauaaaguga ccguuaucaa ggacuggacu aaacggaacg aucauagaca ccaugccaug     2940

gacgcucuca caguggccuu caccaagcca ucguacauuc aguaccugaa ucacuugaac     3000

gcaaggaaag acgaaaauaa caagaauuau uccguaaucc uggcgauaga gaaaaaggaa     3060

acaaucaagg uccccacuaa caaugggaaa aacaagcgag uguuuauuga gccgaucccu     3120

aacuuccgcc agguugccaa aaagcacuua gaggaaauuu uuaucucuca uaaagcuaag     3180

aauaaggugg ucaccaaaaa cacgaauaag cccgccggaa ccaacaaaca acagauaaca     3240

cucacuccac ggggucagcu gcacaaggag accauuuacg gcaaauauca guacuaugug     3300

aauaaggaag agaagguagg ggugaaauuc gauaaggaaa caaucgcaaa aguuagcaac     3360

ccucuuuaca gagaggcucu guugaagagg cuccaagcca augacaacga ucccaaaaag     3420

gcauuuacug gaaagaaugc ccugaguaaa aacccaaucu aucuagacga ggcuaagacc     3480

aaaacgcugc ccgaaaaggu caaucuuaca uaccucgagg aagauuucuc agugcguaaa     3540

aagauugugc cuaaaaaccc aaccuccaag gaguuugucc cgaaucugaa gucuauagaa     3600

aaaguugugg acgagggcgu gcgcaacauc uugauugagc ggcugagagu auacaauaac     3660

gaucccgaaa aggcguucgu cgacuuagag aaaaauccua ucuggcucaa caaggaaaaa     3720

gguauugcca ucaagagggu gacuauaagc gggguuaaca augcacagcc acugcauauc     3780

ggcaaggauc accuuggaaa aacaacccug aacaaggagg gcaaagaaau ucccguggac     3840

uaugucucca cugggaauaa ccaccaugug gcuaucuacc gagauaagga gggaaauuug     3900

caggagcaga uuguuucauu cuuugaagcc gugguacgga cccaacaggg ucucccuauc     3960

auagacaaaa cauauaagca ggaggaaggc uggcaguucc uguuuacgau gaagcaaaac     4020

gagauguucg ucuuuccaaa ugaggccacc ggguucaacc ccgcugaaau ugaccuacug     4080

gauccgaaaa auaagaaaca gaucagcccu aaccuuuuuc gcgugcagaa gaucgcaacu     4140

aaagacuacu ucuuuagaca ccaucucgag acaaaugugg aaaccgauaa cguccugaag     4200

aauacaacuu ggaagaggga gggauugagu ggcuuaaaag acauuguuaa ggugcggauc     4260

aaccaccugg gugauauagu aucuauuggg gaauauuga                            4299


<210>  3353
<211>  4302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3353
augaagaaaa uccugggccu cgaccuuggg accacaagca uuggauacgc ccaggugaac       60

gaguccgaag auuucaagga cucuucaaua gagagaaucg gugucagggu uaauccccug      120

aguguggaug aacaaacuaa cuuugagaaa ggcaagccua uuagcaucaa ugcugaccgg      180

acguugaaau ccggggcacg ccgaaccuua gaucguuauc aggacagaag gucuaaccug      240

auugaugccc uaguaaaguc ggugaucaua acagaagaca cuguccucgc ggaggaugga      300

aagaauacca cacacucaac cuucgcucug cgggccaaaa gcgcaaguga gaagguggaa      360

aaagccgagu uugcucgcau ccuucuggca auuaacaaga aaagaggcua caaguccucu      420

aggaagacua auaacaauga cgaugaaggu caggccaucg acgggauggc uauugccaaa      480

aaguuguaug augagaaccu cacaccagga cagcuggcgu accaacugcu caaagagggc      540

aagaaauauu uaccggacuu cuaccggagc gaccugcagg cagaauuuga uaagaucugg      600

accuuccaga aacaguuuua ucccgagaua cuuacggacg aauugcaaaa ggagcuggcc      660

ggcaaggggc agcgcgcuac uuccgccauu uucuggaaaa aguacgauuu uaauaccgca      720

gaaaacaaag gaacacgaga ggagaagaaa cuaaaggcuu acgaauggag aucaaccagu      780

guugacaaga aacuccagaa ggaggaagug gccuauguca ucacugagau uaauaacaau      840

cugacaaaca gcucugguua ccuuggcgcg aucuccgaua ggucaaaaga acuguauuuc      900

aacaagcaga ccguggggca auacuuguuu aaacagaucc agaagaaucc ucauacgaag      960

cucaaaaacc aaguauucua ucggcaggac uacuuugaug aguucgagau uauaugggaa     1020

acucaggcca aguuccaccc agagcugaca gaagaguuaa agaauaagau ccgugacauu     1080

aucauauuuu aucagcgcaa acugaaaagc caaaagggac ucauuaguuu cugcgaauuu     1140

gagagaaaag agguugaaau cgagaagggc gguaggcgga ugaaaaagac caucgggcuu     1200

aaaguggcac ccaagucuuc cccucuguuc caggaauuua aaauuuggca ggucuugaac     1260

aaugugcuaa uccgaaagaa gggaagcaaa aagcgcagag ugaaagagcc agaucagcug     1320

aaccucgucg agcaagaaca ggagguuaug uucuuugaca ugcagacuaa gcagacacug     1380

uucgaugaac uuaaugugaa aggcaaccug aaggcuaaga ggauuauaga guugaucggc     1440

caagacgcca augacuggga aauuaacuac ucggagaucg aggggaaucg gaccaacaaa     1500

gcucucuacg augccuaucu gaagaucuua gaccuggaag gauacgauac gaaagcacua     1560

cucgacguaa agaccucuaa agaugaggug gaacuggacg auauuaauau auccgguccc     1620

gaggucaagg accuugugaa gcgcaucuuu gaaucauugg agauugauac acagauccug     1680

gaguucgacg ccgaacucga uggcgagguu uuugaaaaac agaguagcua ucugcuuugg     1740

caucuguugu acuccuauga gggggacgau ucuccuagcg gaaacgaaaa gcucuacaaa     1800

cuguuaaagc agaaauucgg cuuuaagaag gagcacgcuc aaauucuggc gucaaucacu     1860

cuaagugacg acuaugguuc ccucagcgca aaagccauga gaaagauaua cccauacauc     1920

agggagcaga ccuucgauaa agcugccucu cuggcagggu aucgucauuc agccaguagc     1980

cuuacaaagg aagagauugc uaaucggccg uugaaagaca agcuggaacu caucaaaaag     2040

aacucccugc gaaaccccgu ggucgagaag auuuuaaauc aggugguaaa caucguuaau     2100

ucucugauag aggccaacuc ggaaaaagau agcgacggca auauuaucuc cuacuucaag     2160

uuugaugaga uuagaaucga acuugcaagg gacuugaaaa agaacgcgaa agagcgcgcu     2220

gaaaugacuc ucaauauaaa cgccgcaaag aucgugcacg agaagauuuu caaacugcua     2280

caagccgagu uuggagucaa gaauccuacc cggaacgaua ucauuagaua uaaacuguac     2340

gaagaacuca agaauaacgg cuauaaagac cuguacacaa auaucuauau accaagggaa     2400

auucuuuucu caaagcagau cgauaucgag cacauuaucc cccagaguaa gauguuugac     2460

gauucuuuca gcaacaaaac cauuguguuu cgcaaggaca auuuggauaa agggaacucc     2520

acugcuuucg acuacauaga cucacgauuu ggaaaggaua aacugacgga cuucgaacag     2580

cggaucgagu uuuuauucga gcaagguaag aagaacaaag aagagggcau uagcaaggcc     2640

aaauaccaga agcuccugaa acgugaagca gauaucgggg acggauuuau ugagagagau     2700

cuuagggaca cacaguauau cgcuaagaag gcccgcaauc uguuguacga aauaugucgg     2760

aucgucaccc cuacuaccgg cucuauuaca gauagacuca gggaggacug ggaucuggug     2820

aacaucaugc aggagcuaaa ucuggacaaa uucaagaaac ucggucugac cgaaaugguu     2880

gagaagaaag auggguccuu caaggaacga auuguggacu ggaguaaacg caacgaccau     2940

cggcaccaug cgauggaugc ccuuacuauc gcuuuuacaa agcacagcca cauacaauau     3000

uugaauuacc ugaacgcaag aaagaaugag aaccauaaac agcaccauaa uauuaucggc     3060

aucgagagga aggaaacgga guucguaauu gacgauuuag gaaacaaaaa gcguaaauuu     3120

caccucccac ugcccaauuu ccgcgaagag gccaagaagc aucuugaaaa cguccuggug     3180

ucuuuuaaag cuaagaauaa aguugugacc aagaacaaaa auaagacugc cuccucaaag     3240

ggcgagaaaa uaaaggucga gcuaacaccg cgggggcagc uccacaaaga aaccguguau     3300

ggaaaguaca aauucuaugu aaguaaggag gaaagaaucg guggcaaguu ugaccagagg     3360

acaauugagc gaguggcaaa cagcacucac aaacggcugu ugcugcaacg cuuacucgaa     3420

aacgauaaug acgcgaagaa agccuucacc gggaagaacg cucuggccaa aaauccuauc     3480

uaccuugaug acacgaagac ccugacauug cccgagauug uuaaacucac uuggcuggag     3540

gaagauuacu cgaucagaaa ggacauaacc ccagagaacu uuaaggauau caaagucauu     3600

gacaagaucc uagaugaagg agugaaaagg auucugaagg agcggcuugc aaaacauaau     3660

aacaauccua aggaggcuuu cuccgaccuc gagaagaacc ccaucuggcu gaaugagaaa     3720

aagucuauaa gcauuaaaaa ggugaaaauc ucaggcauca acaaugccga agcauugcac     3780

uauaagaaga accaucuggg uauugagauc uuagacgaug agaaaaagcg aauaccaguc     3840

gacuuuguua guacagggaa uaaccaccac guggccauuu acagagauga aaaaggcaau     3900

cuccaggagg acgugguauc cuucuucgaa gcugucgcgc gcgugaacca gaaucugccc     3960

aucauugaua agacucuuaa agccuaucug ggauggaccu uuuuguucac gaugaagcaa     4020

aacgaguacu uuaucuuccc uaacaagcag acaggcuuua auccaaccga aauagaccuc     4080

cuggauucua ugaacaauaa acuaauuagc ccgaaccugu ucaggguuca gaaguuuacu     4140

gugaaagacu auuucuuucg ucaucaccuu gagacaaaug ucgaagauga cuccaagcuc     4200

aaaaacacca cuuggaagcg ggaggggcug ggaggcaucg guaauaucgu gaaaguuaga     4260

auuaaccaua ucgggcagau ugugaagaua ggagaguacu ga                        4302


<210>  3354
<211>  4305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3354
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugggc cuacgugaaa       60

caggcucaaa acgauuccga gaagucugaa auagcagagc ugggugucag aguuaauccc      120

uugacagugg acgaaaagac uaacuucgag aagggcaaac cuaucucaac gaaugccgau      180

aggaccuuaa agcgcggggc gcggcgaaac cugcagcguu auaagcuaag aaggcaggaa      240

cucauugagc ugcuuaaaga cuaccaggua aucagugaua agacaauucu ggcugaggac      300

ggaaaagaaa cuaccuuuga gacauugcgg cuccgcguga aaagcgccaa agaacaaguc      360

ucccuggagg agcuggcaag aauccucuua augauaaaua agaagagggg cuauaaaucu      420

ucgcggaagg ccaaaaacga agaugagggu cagcugaucg acgggaugga aauugcucgc      480

cgacuuuacg augagaauuu gaccacugga cagcugucac uacagcuccu gaaggaaggc      540

aaaaagagcc uuccagacua uuacagaagu gaucugcaac acgaguugga caaagugugg      600

aacuuccaga agcaguuuca uccggauauc cucacagacg aguucuauac cuccauucag      660

ggcaaggggc aaaaagcaac gagggaaucu uuuagcucca aguacggaau cgacacugcc      720

gagaauaaag guucacggga agagaagaaa cugcaggcuu accaguggcg ugccgaugcg      780

acccacaagg aauuaacaaa ggaggagcug gcauucguua uaacugaaau uaacaauaac      840

aucaauaaca guagcggcua ucucggggcc auuucugacc gcuccaaaga gcuuaucuuu      900

gcuaacgaaa ccgugggaga guaccugugg aagcaaaucc agaaugaucc ccauacauca      960

uugaaaaacc aggucuucua uagacaggac uacuuugaug aguucgaaag gauuugggag     1020

acccaaagcg gcuuuuaucc ucagauacua acggaaaguc ugaagaauga gcuccgggac     1080

auccugauuu ucuaccagcg aaaacuuaag ucucagaagg cccugguguc caucugcgaa     1140

uuugagagca aagagauacc auugaaggau aaacaaggua accccauuuc gaagaauggg     1200

aucccuguaa cuaaacucgu uggaccaaag guggcaccca agucuucccc ucuguuccag     1260

gaguucaaaa ucuggcagaa cuuaaauaac guccuggugc gcccaaaggg cucaagaaaa     1320

aggaagguga caaccaauca gcaaaguacu cuacucgaag gccaggaacc ggucuuugag     1380

cugaaccuug aacagaaaga guugcuguuc gaggaacuca aucugaaggg gaaccuuaaa     1440

acagacuauu gucuggaguu gauuggauac agcgcuaagg aaugggagau gaauuacccc     1500

acccucgaag guaacaagac gaauaaagcc cuguaugagg cguacuuaaa gaucauugau     1560

uccgagggcu augacgccaa aaagcugcua aaaguuaaga ccgauaagga cgacauaaac     1620

cucgaugaca ucaaaguguc ugcaagcgaa auuaagacaa ugaucucaga uaucuuuaac     1680

gagcugggga uuaauacuaa aauacuugac uucgaugcug aauuggagcg gaagcaguuu     1740

gaagcccaag cuaguuaccg ccuguggcac cuccuguauu ccuacgaggg agacgauagc     1800

aaaucuggca acgaguuacu guauagacuu uuggaagaca aguucgguuu uaagagggag     1860

cauucaaaaa cccucgccaa uguaagucug cuaagcgauu acgggaaccu guccacaaag     1920

gcaauccgua cuauuuaccc uuauaucaaa gaaaauaagu ucucuaccgc cugcgagcuc     1980

gcuggcuacc ggcacucgaa acugagcauu uccaaggagg aaaacgagaa ucgaacacuu     2040

aaggacaccu uggaugugcu gaaaaagaac ucauuaagac agccagucgu ggaaaagauc     2100

cucaaucaga ugauaaaccu gaucaaugag cuuauugaaa agcacaguga gaaagacgag     2160

aagggaaaca ucguuaaaua uuuuaaguuc gaugaaauuc auaucgagcu ggcgagggaa     2220

uugaagaaaa augcagagga acgccgggag cucgacucua aggugagaga gggcaaagac     2280

aggaacgaaa agauaauuac uauccugaaa agcgaguuug ggcuacccua cccuacgaag     2340

aaugauauua cacgcuaucg acuguacgag gaacucaagg acaacggaua uaaagaucug     2400

uacaccaaca cuuacauccc acgggaaaaa cuuuucucca aagagauaga caucgagcac     2460

auuaucccca agucacguuu guuuaaugau agcuucucua acaaaaccau uuguuauaga     2520

caagacaauc ugaacaaggg ugaaaggaca gccuacgauu auaucgaggg cuuagggaag     2580

acccagcucg acgaauaccu gaaucgcguc gagaaccuuu auacuaauaa auucauaaca     2640

aaggcuaaau accggaaccu gcuaaagaaa gaguccgaaa uuggagaugg cuuuaucgag     2700

agagaccuca gggaaacgca guauaucgcc aagaaggcaa aagagaugcu guugcagauu     2760

acccgacaug ugguaaguac uacagguagc aucaccgauc gccugcggga cgauugggaa     2820

uuaguuaaug ugaugagaga gcucaaccug ccgaaguaca aaguccuugg gcugacagag     2880

auucaagaaa ggaagaaugg ccagacugug gaggucauaa ccgacuggac gaaacguaac     2940

gaccaccgcc accaugccau ggaugcuuug accguggccu ucacaaagca cucucauauc     3000

caguaccuca auuuucugaa cgcacggaag gacgaaaaac acaaggagua uuccaauauu     3060

augggaaucc agcaacuaga aacugagaaa accacagaua aggacggcaa agauagaagg     3120

guuuucaaga cuccuauucc caacuuucga cagguggcga aagagcaucu ggaaucaauc     3180

cuuauaaguc acaagaccaa gaacaaagua gucacgcggg cuacaaauaa gaucgccggg     3240

agcaaaaagc agcaggagac ccucucgcca cgcggacaac ugcacaaaga aacuguguac     3300

gguaaguaua gaagguacgu ucagaaggag gagaaaauug gcuccaaguu cgacuugcag     3360

acaaucgaac ggguggcaaa cccucuguau aaacgauuac ucaccaagag acuggaggaa     3420

aaugggggag aucccaaaaa ggccuuuacu ggcaagaacg cucuugccaa aucuccaauu     3480

uaccuggacg agacccaaac acagcaguug cccgaaaagg ucaaacucgu guggcuggag     3540

acggauuuca gcauccgcaa ggacauaacc ccugauaauu uuaaagacga gaagucaauu     3600

gacaagguac uagaugaagg uaucaaaagg auccugaagg accgucuuaa aaguuucgau     3660

ggggacgcaa agcgggcuuu uacugaucuc gagcagaacc caauuuggcu gaauaaagaa     3720

aagggcauag ccaucaaaag agugacaauu uccggaguuu cuaacgcgga gccguugcau     3780

accaagaagg accacuuagg caaugaaauc uucgaugaca cagggaaacc cauuccuguc     3840

gauuauguga gcacuggaaa caaucaucac aucgccauau acagggacga gaacggcaau     3900

cugcaagaga aggcuguguc cuuuuucgaa gcagucaccc gcgccaacca gggucuccca     3960

acgguugaua aaacauacaa ucagcaccug ggguggcagu uccuuuuuac caugaagcaa     4020

aacgagaugu ucguguuucc caauaaagaa acuggauucg acccucagga gaucgaucug     4080

uuggacccaa agaacaagaa acuaauuuca cccaaccugu uucgagugca gaagaucagc     4140

accaaaaauu auuucuuuag acaucaccuc gaaacaagug uagagacuca uaacgcucug     4200

aauggcguca ccuacaaguc ucagcuuggu uuaaacggca uuacagacau cauaaaagug     4260

cggauuaauc accuggggga uaucguucau aucggagagu auuga                     4305


<210>  3355
<211>  4308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3355
augcugggcu gcgagagaaa gaggaaacgg cgcgaaaagg agaaaauggc caagcccaaa       60

gacuacacca ucgggcucga uauuggaaca aacagcgugg guugggucgu uacugacgau      120

cagaauaaca uacuucgaau caagggcaag aaagcuauug gggcacgucu guucacggaa      180

ggaaaggugg ccgcggagag aagguccuuu cggaccacac gcagaagguu gucucggcgc      240

cgauggagaa ucaaaauguu agaagaacug uucgacgagg aaauugcuaa gguagauccu      300

ucauuuuucg ccaggcuaca cgagaguugg aucagcccaa aagacaagcg gaagcguuau      360

uccgcaauag uguuuccguc ucccgaagag gauaaaaagu uccaugaguc guacccuacu      420

aucuaucacc uccgcgacaa acugaugaag gaugaccaaa aacaugauau uagagaaauc      480

uacauugccg uccaccagau gaucaaggcu aggggcaauu uucuucauga cgagucagug      540

gaaaccuauc ggagcgguau gaguucccug ggagggcgau cugagcgcaa cauauugagc      600

guucagacac ucgaagaacu gaaugaucug uucgcagaga acgaaggcac cgaggaagug      660

gagcucaaug ucgccuccgc ugaacagauu aacgacaucu uaacuggcgg gcaccugaau      720

gccgacucac aaaaagagau uaguaaccuu uugcugccaa gcucuuuucc cuccuucgau      780

gacaaggcga aggagaaaca ggugaagaaa cuaaucaaua acguagcaac aaauaucuca      840

aaggccuggc ucggauacaa agcugauuuu agcaccauuc ugaaccuugc caagguugac      900

aaggaucaga aaaagauauu cgcauuugcu cugcagggug gcgacgaaga ggauaaagug      960

caagaauugg agagucuccu ggaacagucu cagacggaca ucgucgauag auuaauugag     1020

aucaggcacg ccauagugcu guccgagauu gugccugucg ggaugacucu cagcgaagcg     1080

augaucgaca aguaugauca gcauaaagag gaccuuauca cccugaaggc cguuauucgg     1140

aauacaaagg acaaaaagaa agcagcuaag uugcaagcca ucuacgaucu auacgugaaa     1200

aaccgccacg cugaccuggc caaggcaaug aagcucacug gaauuaaaaa gagaucggaa     1260

cugcuugauc cagaggagcu gaaaaagggc auaucuuccu ugcucgacga uucacccgaa     1320

gccguagaga ucaaacagag gcuggaagag aagaccuucu uaccucugca gcguaguaac     1380

aauaacggug ugauuccaaa ucaacuacau caggucgaac ucgacgagau caucaaaaag     1440

cagagcaagu auuacccguu ccuggcugag aaaaaccccg augaauccga ggaagcgcag     1500

aagaaagcac cuacaaaacu ugacgccuug cugaccuuuc gggugccaua uuacguuggg     1560

cccaugauua cgaaagagga acaagaggcu cagaauggac acucuuucgc cuggauggug     1620

cgaagagauc cucaggacca cgaggcaaua acuccaugga acuuugaaaa gaaggucgau     1680

aaaauggcca gcgcuacaca guucaucaag aggaugacca cuaaagacac auaucuccug     1740

ggcgaggaug ugcuucccgc cucaagucug aaguaccaau uguuuaccgu acucaaugaa     1800

cugaacaauu uacgcguuaa cggcaaaaag cugacguccg acgagaagga acaggugauu     1860

gaggggcuau ucaaaaagca gaaaaccguc aaggcagaca aauuugugaa guauuggcag     1920

gcgaagcaua ucggagcuga uauuaaaguc aagggucuca gcgacccguc uaaauucaau     1980

ucaacaauga guacuuacau cgauuuuaag aaaauauucg gcgaccaacu gaacgaugug     2040

aaucggcaga acgacuuuga gaagaucauu gaauacagca ccaucuucga ggauagaaaa     2100

auucuugaag acaaguugag gguugauuuu gccuggcugu ccgaggagca gaucaagcag     2160

cucugucgcc ugcgauuaca agggugggga cggcugucug acaaacuuuu gacacgucuc     2220

acugaugcag acggccagaa ugugcuggaa agacuaugga acucgaacga uaauuucgua     2280

cagauaguca gcgacccuaa cauuaaggcc aaaaucgagc aagaaaauca gaagcugcuc     2340

agggacuccc agucagauaa cgcuguggag aguauucugg acgaugccua uaccucuccc     2400

cagaauaaaa aggcaauccg ccaaguuaug cggguggucc acgacauagu gaaggcuugc     2460

gaugguaacg uaccagccaa auucgcgauc gaauuugcca gaagccagga ggacgauccu     2520

aagaggacaa ccgcucgagu gaaucagaug cagaaaauuu acgaccaaau cuccgaugag     2580

guugucucac agggggugaa ggaacagcuu agcggcauga aaucuuugaa ggaccgcuau     2640

uaccuguauu ucaugcaggg aggccgggau gcauacacug gggccaaguu aaacauugac     2700

agacucuccg acuaugaugu ggaccauauc augccccaaa guuuugucaa agaugacagc     2760

cuggauaaua ggguucuugu ggcucgugcc gugaacaauc agaagucuga caaaguacca     2820

gcacuguugu ucggaaacaa ggucguggcg gaucucggua uaacgguucg cgagaugugg     2880

gacaaauggc agaagcuggg caugauuucc aagcggaaac uaucaaaucu gcucacagau     2940

agugacgccc ugaccgaaua cagagcucaa ggguuuauca ggcgacagcu uguggagacu     3000

agccagguca ucaaguugac cgccacaauu cugcagucgg aguuccccga uuccaaaauc     3060

auugaggugc cugcaaagua caacucuaua guucggaaac aauuugacuu auauaagagc     3120

cgcgagguga augauuucca ccaugcuauc gacgccuacc ucucaaccau uguaggaaac     3180

uaucuguacc aggucuaucc aaaccuuaga aggauguuug uguacggcga guucaagaaa     3240

uuuaguucca augcagagga aucugcccac gacguggcuc ggcgagucaa gagcaugaac     3300

uuccuggaug accuacucag agguacucac ggggauaaua ucuauugucg cuccacaggc     3360

gagauuguuu uuaacaggaa ugacaucaua ucaaaacuga agcaggcgua cagcuucaaa     3420

cagauguugg ugacgcaaga aguauucacc aagaaaagug cccuguuuaa ccagacugug     3480

uacccgucuc ccaagcguga uggauccaag cggagcggcu uaaucccuag aaaaaggggg     3540

auggacccag agauuuaugg agguuacgca cauaaucagg auucguauuu cguccucgcu     3600

aagaucgaga aagugaaggg cuacacacug cagauuguug gggugcccau ccgcucacuu     3660

gaaauaauua acaaagccga ggacuuuucu accgcccugu uggauaucau caagaguaag     3720

gucauguaua augaaaagac aggacaaccu aagcgaggca uuaaaaacgu ggagauacuc     3780

guuaauaagc ugccauacag acagcccauc cuagagaaag acguguccua uaugcugggu     3840

agcucugcuu acaaguacaa cauuaagcag aucacucuua gccggcaguc caugaaauau     3900

auucucgauu acaucgacga uccgaauuuc accaagcacg aaaugacgau gauagacgag     3960

caaaacgaaa gggagugccu guugucaguc uaugacgaaa uccuggagaa aguagauaag     4020

uacuuaccuc ucuuugacgg gaauaaauuc cgcgagaagc ugcauagugc acguaaggaa     4080

uuugugagcc uuucuguuga ugacgaggcc uccgcgauug agaaaauccu gagaggcuug     4140

cacgcuaacu cagaaaugcc aagucucgaa aaccugaaga uuaaacccgc ccuaggauuc     4200

uuucagauga agaauggcau cacccugagc aacgaugcag ugcuucucua ucaguccccu     4260

acagggcugu ucgagcggag ggucaaagug ucugacuugu uaaaguga                  4308


<210>  3356
<211>  4308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3356
augaagaaca uccugggccu cgaccuuggg accacaagca uuggauucgc ccacauagug       60

gaggaugaaa auaaagagaa guccgaaauc aaagagcugg gugucagaau uguucccuug      120

acuacggacg aacagucuga uuuugagaag ggcaaaucaa ucaccacaaa cgcuaauagg      180

acuuuaaagc auggggcacg gcugaaccua gaccgcuacc aacagcgacg uaaguaucuc      240

auugaucugc uucagaaagc caaucugauc accaguagcu ccauauuggc ggagaacgga      300

aagaauacaa cccacucuac uuggcagcuc agagcuaaag ccgugacaga aaggaucgag      360

aaggaagagu ucgcacgggu acugcuggcc auuaacaaaa agcgcggcua caagucguca      420

agaaaagcua agaccgagga cgaaggucaa gcaaucgaug ggauggccau ugcuaaaagg      480

cucuaugacg agaauuuaac gacuggacag cugagccuuc aguugcugca gcaaaacaag      540

aaacuacucc cugauuuuua ccggagugac cugcagaagg aguucgaucu uguguggaau      600

uuucagaaac aguucuaucc agacauccug accgauuccu uuuacaagga auugcaaggc      660

aagggcaaag acgccacauc uaaggcguuc agcaaacgcu aucauuuuga cacugcagaa      720

aacaaggggu ccaaagaguc aguccgacuc caggccuacc aguggagagu ggaggcuaua      780

aguaagcaac ugagcaagga agagguugcc uacguguuaa ccgaaauuaa uaacaaucug      840

aacaacgcau cuggauaucu cggugcuauc uccgauaggu caaaagagcu uuacuucaau      900

cggcagacag ucggccagua ucuguacgcc caguugcaag aaaaccguca caauagccua      960

aagaacaaag uguuuuaucg ccaggacuac cuggaugagu ucgagagaau uugggaaacc     1020

caggcgaguu uucauaagga gcucacggag gaacugaaaa agcagaucag ggacguaguu     1080

aucuucuauc aacggaagcc gaaaucucag aaggggcuua uuuccuucug cgaauuugag     1140

agcaaagaga uagaaaucga gaaggaugga aaaacuauua caaagaccau cggcgcccga     1200

guggucccca agucgucucc ucuguuccag gaauuuaaaa uauggcagau uuugaauaac     1260

gugaucugua agcgcaaagg uaucagaaag aaaaaggugu ccgcaaaaac uacacaacuc     1320

gaccuguuaa augagucaag ucagaccauu uucagccugg auauggagug caagcagcua     1380

cucuuugagg aacugaaccu uaagggggac uugaaauccg auaagguccu gaaacuccug     1440

ggauacucuc cacaggaaug ggagaucaau uacaaccaac uugaaggcaa uaggacgcag     1500

aaggcucugu augaggccua cuugaaaauu guugagaugg aagcucacga cgugaaggau     1560

auacuccaga ucaagagcgc caaagacgac uggucacugg augagagucc cuuauccgca     1620

agcgaaauuc gggagucugu aaaggccauc uuccaagcuc ugggcaucug uaccaaaauu     1680

cuauauuuug acccucuccu gccagugaag gaguucgaaa aacaggauuc auaccagcuu     1740

uggcacuugc uguauaguua cgagagcgac gauuccacau cugggaacga aacucucuau     1800

cgcauacugg agaagaagua cgcguuuaaa agagaacaug ucaggaucuu agcaaaugug     1860

gcccugcagg acgauuacgg aucgcuuagc accaaggcua uucguaaaau cuaucccaac     1920

auuaaggaga aucaauacuc caucgccugc gaaauggcag guuauaaaca cucaaaguug     1980

agucucacaa cugaggagcu ggaagcccgg gagcuaaaga acauaauccc ucugcucaaa     2040

aagaacgcuc ugcgaaaucc aguuguggaa aagauucuua accagaugau caaugucgug     2100

aacgccuuga uugagaaaaa uucugagaga gacgcagaag gcaaaaucac caaguacuuc     2160

cauuuugaug agauaaggau ugaacuggcg cgcgagcuca aaaagaacgc ucagaagcgg     2220

uaugaaauga cacagaauau caacaaagcc aaguuagagc accagaaaau uagcgagauc     2280

cugcaaaagc aguucgggau caaaaauccg accaaguccg acauaauuag auacaggcuu     2340

uaucaggaac uggagcacaa cggauacaag gaauuguaca cuaaugcaua uaucgccccc     2400

gauguacucu uuagcaaaaa cauugacauc gagcauauag uuccuaaggc ucgcguguuc     2460

gaugacucau ucucuaauaa aacgcuggcc uuucgagagu ucaacauuaa gaaaggcgaa     2520

uccacagcau uugacuacau caccagucua aauagcgagg aggaacugaa ccaauaucuc     2580

acucgggucg aaaacgcuua caagaccaag ucuaucuccc caacaaaaua uaagaaucug     2640

cuuaaaaagg ccucagagau uggugaugac uucaucaacc gugauuugag agacacccag     2700

uacauugcga aaaaggccaa ggaaauacug uuucagguga cuaaaaaugu cuuaaguaca     2760

agcgggucga ucacggauag gcuccgcgag gacuggggcc ugguggaugu uaugaaggag     2820

cuuaacaugc ccaaauauca aucccuggga cuaaccgaag uggaggaacg gaaggacggc     2880

aauaaaguaa cugucauuaa gaacuggaca aagagaaaug aucacaggca ucacgcuaug     2940

gacgcacuca ccguggccuu cacaaaaccu ucuuacaucc aguaccugaa ccauuugaau     3000

gcucgaaagg augagaacaa uaaaaacuau agcguuauuc uggccaucga ggaaaaggag     3060

acuauaaaag ugcgcaccaa uaacgggaag aauaaacggg ucuuuaucga accaauuccc     3120

aacuucagac agguggcaaa gaagcacuua gaggaaaucu uuauuucaca caaaacgaag     3180

aacaaaguag ugaccaagaa uguuaacaaa ccggcgggaa cagacaagca gcaaaucacu     3240

cucaccccua ggggucagcu gcauaaggag acaauauacg gcaaauauca guacuauguc     3300

aauaaggagg agaaaauugg ggugaaguuc gauaaagaaa cuaucgccaa ggugaguaac     3360

ccccuuuacc gugaagcucu guugaagcgc cuccaggcca augacaacga cccuaaaaag     3420

gcauuuaccg gcaaaaaugc ucuguccaag aaaccaaucu aucuagauga gucuaagacg     3480

aagacacugc ccgaaaaagu caaccuuacc uaccucgagg aggacuucag cauucggaag     3540

gauauauccc cagacaauuu uaaagaucug aagucaaucg aaaaaguuau ugaccaagga     3600

gugaagagaa ucuugauuaa aaggcugcag gccuacgaua acgaccccaa gaaggcauuc     3660

guggauuuag agaaaaaucc uaucuggcuc aacaaggaaa aagguauagc caucaagcga     3720

guaacuauua gcggggucaa uaacgcucag ccacugcaca ucggcaaaga ccaucuugga     3780

aagacaaccc ugaauaagga gggcaaagaa auuccggugg auuauguuag uacugggaac     3840

aaucaccacg uggcgaucua ccgggacaag gagggaaacu ugcaggagca aauagucucu     3900

uucuuugacg ccgugguucg cgcacagcag ggucucccca uuaucgauaa aaccuauaag     3960

caggcugaag gcuggcaauu cauguuuaca augaaacaga acgagauguu cguguuuccu     4020

aaugaaacga ccggguucaa cccagccgag aucgaccugc uagaucccaa gaauaagaaa     4080

cugauuuccc cuaaccuuuu uagaguacag acuaucagcg ucgugaagua cggaaauucg     4140

guggucaggg acuucaaauu ucggcaucac cucgagacaa cccugcaaga ugacaagaua     4200

uugcagggca uuacauucca gcagaucaaa ucauuagaac cacugcgaaa cauuguuaag     4260

gugagaauca aucaucucgg ugauauagua ucuauugggg aguauuga                  4308


<210>  3357
<211>  4308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3357
augaagaaca uccugggccu cgaccuuggg accacaagca uuggauucgc ccacauagug       60

gaggaugaaa auaaagagaa guccgaaauc aaagagcugg gugucagaau uguuucuuug      120

gcuacugacg aacagucaga uuuugagaag ggcaaaagua ucagcacgaa cgcaaauagg      180

accuuaaagc auggggcccg gcugaaccua gaccgcuacc aacagcgacg uaaguuccuc      240

auugaucugc uucagaaagc gaaucugauc acauccgcuu cuauauuggc cgagaacgga      300

aagaauacua cccacucgac auggcagcuc agagcaaaag ccgugaccga aaggaucgag      360

aaggaagagu uugcucgggu acugcuggca auuaacaaaa agcgcggcua uaaaaauuca      420

agaaaaacua agacagagga cgaaggucaa gccaucgaug ggauggcuau ugccaaaugc      480

cucuacgacg agaacuuaac ccccggacag cugagccuuc aguugcugca gcaaaauaag      540

aaacuacucc cugauuucua uaggagugac cugcagaagg aauuugaucu uguguggaac      600

cugcagaaac aguucuaccc agacaucuug acggauuccu uuuauaagga gcuccaaggc      660

aagggcaaag acgcgacuuc uaaggcauuc agcaaacggu accauuuuga caccgccgaa      720

aauaaggggu ccaaagaguc aguccgccug caggcuuauc aguggcgagc cgaggcaaua      780

aguaagcaau uaagcaagga agagguggcu uacguucuga cagaaauuaa caauaaccuc      840

aacaauucuu ccggauaccu uggugccauc ucagauagaa gcaaagagcu guauuucaac      900

aggcagacug ugggccagua cuuguaugcg cagcuacaag aaaaucggca caacagucug      960

aagaauaaag ucuuuuaccg ucaggacuau cucgaugagu ucgagcgcau uugggaaacc     1020

caggccucuu uucauaagga gcugacagac gaacuuaaaa agcagaucag agauguggua     1080

aucuucuacc aaaggaagcu gaaaucccag aagggguugg uuagcuucug ugaguuugaa     1140

ucgaaagaga uugagauaga aaaggacgga aaagcaauca ccaagucuau uggcgcucgg     1200

guggucccga aguccucacc ccucuuccag gaguuuaaaa ucuggcagau acugaacaau     1260

gugacgugca agcgaaaagg uauucgcaag aaaaagagua gcgccaaauc cacugaauua     1320

gaucuguuca acgagccuuc ucaaagcauc uuuucacuag acauggagug uaagcagcuc     1380

cuguucgaug aacuuaauuu gaagggggac cugaaaagug auaaggugcu caaacugcuu     1440

ggauauuccc cacaggagug ggaaaucaac uacaaucagc uggagggcaa cagaacacaa     1500

aagagcuugu acgaagcaua ucucaaaauu aucgagauug agggcuacga agucaaggac     1560

auacugcaga ucaagucuac caaagacgau uggucauuag acgagagucc ccugagcgcc     1620

uccgaaauua gggagcgggu uaaggugauc uuucagacuc uagggaucug cacaaaaauu     1680

cucuauuucg auccucugcu uccaguaaag gaauuugagc aacaguacuc uuaucaguug     1740

uggcaucugc ucuacucgua ugagagcgac gauuccaccu caggaaauga aacgcuguac     1800

cgcauauuag agaaaaagua cgcauucaag agagaacacg ccaccauccu ggcuaacgug     1860

gcgcuucagg acgauuaugg uaguuugucu acaaaagcaa uuaggaagau cuaccccuau     1920

auuaaagaga aucaauacag cacugccugu gaacucgcug gcuauaagca cuccaaacug     1980

ucacuaacca cagaggagcu ggaagcccgu gagcucaaga acaucauacc ucugcuuaag     2040

aaaaacgcau ugcggaaucc agucguggaa aagauccuga accagaugau uaauguugug     2100

aacgccuuaa ucgagaaaaa uagcgagcga gacgcugaag ggaagauuac uaaauacuuu     2160

cacuucgaug agaucagaau agaacucgcc agggagcuga agaaaaacgc acaggaacgc     2220

uaugagauga cccagaauau uaacaaggcg aaacuugagc aucaaaagau cucugaaaag     2280

cugcagaaac aguuuggaau uaagaauccg acaaaauccg acaucauacg guacagauug     2340

uaccaggagc ucgaacacaa cggcuauaag gagcuguaca ccaauacuua uaucgcuccc     2400

gauguccuau ucaguaagaa cauugacauc gaacacauug ugccuaaagc caggguauuc     2460

gaugacagcu uuucuaauaa gacgcuggca uucaaagagu uuaacaucaa gaaaggugag     2520

uccacagccu ucgacuacau aaccucacuc aauugcgaag gggagcugaa ccaauaucuu     2580

acucgcguug aaaacgcuua caagaccaag aguauuagcu ugacaaaaua uaagaaucug     2640

uuaaagaagg ccucggagau cggcgaugga uuuaucaacc gagaccuccg ggauacccag     2700

uacauugcua aaaaggcuaa ggaaauccug uuccagguga cuaaaaaugu ccuuuccaca     2760

ucuggcagca uuacggaccg ucugagagag gauugggggc uaguggacgu caugaaggag     2820

cucaacaugc caaaauacca aucacuggga uugaccgaag uggaggaaag gaaggauggu     2880

aauaaaguua cugugauaaa ggacuggaca aagcgcaacg aucaucggca ccaugccaug     2940

gacgcgcuga ccguagccuu uacaaaaccc aguuauaucc aguacuuaaa ucaccucaac     3000

gcuagaaagg augagaauaa caaaaauuau uccgucauuc uggcaaucga gaagaaagaa     3060

acuauuaagg ugccuaucaa caauggcaaa aacaagaggg uuuucauaga gccaauccuu     3120

aauuuucgac agguggccaa gaaacaucug gaagagauuu ucaucucuca caaggcuaaa     3180

aacaaggucg ugaccaaaaa cauuaauaag cccgccggga cggacaagca gcaaaucacc     3240

uugacaccgc gcggacagcu ccacaaagaa acuauauacg gcaaguauca guacuaugua     3300

aacaaagagg agaagguggg uguuaaauuu gaugaacgga ccauuacaaa ggucagcaau     3360

ccucuguaca gagaggcacu acugaagagg cuucaggcga acgacaauga ccccaaaaag     3420

gccuucgcug ggaaaaacgc ccucuccaag aauccaaucu accuggauga agcaaaaacu     3480

aagaccuugc cugagaaggu gaaccugacg uauuuagaag aggacuuuuc aauccguaaa     3540

gauauuagcc ccgacaauuu caaggaucuc aaaaguauag agaaggugau cgaccaaggc     3600

gucaaacgca uucugaucaa gcggcuucag gcuuacgaua acgaccccaa aaaggccuuc     3660

guugaucugg aaaagaaucc aauuugguug aacaaagaga agggaaucgc aauaaaaaga     3720

gugacaaucu cuggcguguc caaugcccag ccucuccaua uugggaagga ccaccuggga     3780

aaaaccacuc uaaacaagga agguaaggag guaccagucg auuaugugag cacaggcaau     3840

aaccaucacg uugcuaucua cagggacaaa gaagggaauc ugcaggagca aauugugucg     3900

uuuuucgagg cggucgugcg agcccagcag ggacuuccga ucauagacaa gaacuauaaa     3960

caggcagaag gcuggcaauu uauguucacc augaagcaga acgagauguu uguuuucccc     4020

aaugaagcua cugguuuuaa cccugccgag auugaucucc uggaccccaa aaauaagaag     4080

uugaucucac caaacuuauu ccgggugcag accaucucua uuguaaaaua cgggaauagu     4140

gucgugcgcg auuuuaaguu cagacaccau cuggagacaa cgcuccaaga cgauaaaauc     4200

cugcagggca uaaccuuuca gcagauuaag ucccuugaac cucugaggaa caucgugaaa     4260

guccggauua aucacuuggg agacaucguu aacauaggcg aguauuga                  4308


<210>  3358
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3358
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acaaagauga gagguccucu auagucaagu uggguguucg ggugaauccc      120

uuaacaguag acgaacugac uaacuucgag aaaggcaagu caaucacgac caaugcagau      180

cgcacacuaa aacgagggau gcguagaaac cuccagaggu acaagcugcg gcgcgaaacu      240

cuuaccgagg ugcugaagga gcacaaauug auuacagaag acaccauccu cagugagaau      300

ggaaacagaa cuacauuuga aaccuauagg cugcgggcca aggcggucac ggaggagauu      360

agccuggaag aguucgcucg cgugcucuua augaucaaua aaaagcgagg cuacaaaucc      420

ucuagaaagg ccaagggugu ugaagagggg acucugauag auggaaugga caucgcaagg      480

gaacuuuaua acaauaacuu gaccccuggc gagcugugcc uacaacuccu ggaugccggc      540

aaaaaguuuc uuccagacuu cuaccggucg gaucugcaga augaguugga ccguauuugg      600

gaaaaacaga aggaguauua cccggaaauc cucacagaug ugcugaaaga ggaauuacgc      660

gggaagaaaa gagacgcugu cugggcaauu ugugccaagu auuuugugug gaaggagaac      720

uacacugagu ggaauaaaga aaagggaaaa accgagcagc aagaaaggga gcauaagcug      780

gaagguaucu acucaaaacg gaagcgagau gaggcuaagc gcgagaaccu ccaguggaga      840

guaaacggcc uuaaagaaaa gcugagcuug gagcagcuag uuauaguguu ccaggaaaug      900

aauacacaaa uuaacaauag uuccggguau cugggagcca ucucugacag gagcaaagag      960

cucuacuuua acaagcagac cgucggccag uaucagaugg agaugcugga caaaaauccc     1020

aacgcguccc uucggaauau gguguucuac cgccaagauu aucuggacga auuuaacaug     1080

uugugggaga agcaggcagu guaccacaag gaacucacgg aggaacugaa aaaggagauu     1140

agagauauca ucauuuucua ucagaggcgu uuaaaaucac aaaagggucu gauaggguuu     1200

ugcgaguucg aaagucggca gaucgagguc gacauugaug gcaaaaagaa gaucaaaacu     1260

guuggaaauc gagugauaag cagaucuucc ccucuauuuc aggaauuuaa gauuuggcag     1320

auccucaaca auaucgaggu aacagugguc ggcaaaaaga ggaaacgccg gaagcugaaa     1380

gaaaacuacu cagcccuuuu cgaggaguug aaugacgcug aacaacugga gcucaacggg     1440

agcagaaggc ugugucagga agagaaggaa cuucuggccc aggaguuguu ugugcgcgau     1500

aagaugacca aaagugaggu ucucaagcug uuauucgaca auccacagga acuggaucua     1560

aacuuuaaaa cuauugacgg aaacaagaca gguuacgcac ucuuccaggc uuauucuaaa     1620

augaucgaga uguccggcca ugaacccgug gauuuuaaga agccugucga gaaaguggua     1680

gaguacauua aggccguuuu cgaccugcuu aauuggaaca ccgacauauu gggguuuaau     1740

agcaacgaag aacuggauaa ucaaucguau uacaaacucu ggcaccuguu auauucuuuc     1800

gaaggagaca acacgccaac cggcaauggu cgacugaucc agaagaugac agagcuuuac     1860

ggguuugaaa aagaguaugc gacuauuuug gccaacgugu ccuuccagga ugacuacggc     1920

ucacucagug caaaggcuau ccauaagauc cugccccacc uaaaagaggg aaaucgguac     1980

gaugucgccu gcguguaugc uggcuaccgu cacagcgaau ccucucugac cagagaggaa     2040

auugccaaca agguccucaa agacaggcug augcuuuugc cuaagaauag ccugcauaac     2100

ccagugguug agaaaauauu aaaucaaaug gugaacguaa ucaaugucau uaucgauauu     2160

uaugggaagc cggacgaaau ccgcguggag cucgcacggg agcugaagaa aaacgccaag     2220

gaaagagagg aacuuacaaa aucaauagcu cagacuacca aggcgcacga ggaauacaaa     2280

acacuguugc agaccgaguu uggacucacu aacguuagua ggacggauau ccugcgauau     2340

aagcuauaca aagagcugga auccuguggu uauaagacac ucuacagcaa uaccuacauu     2400

ucucgcgaga agcuguucuc aaaagaauuu gacaucgagc auauuauccc ccaggcacgg     2460

cuuuucgaug acaguuucag caacaagacu uuggaggcca gauccgugaa uauagaaaaa     2520

ggcaacaaua ccgcuuauga uuuugucaag gagaaauucg gggaaucugg agccgacaac     2580

ucgcuggagc aauacuuaaa uaacauugaa gaccucuuua agagcggcaa gaucuccaaa     2640

acaaaguaua auaaacugaa gauggcagag caggauauuc cugacgguuu caucgagagg     2700

gaucuucgua acacccagua cauagccaaa aaggcucugu caaugcuaaa ugaaaucagu     2760

caccgcgugg uagccacuuc ugggagcgug acagacaagc uccgggagga uuggcaacug     2820

auugacguua ugaaagaauu gaacugggag aaguauaaag cacugggcuu agucgaauac     2880

uuugaggaua gagacggaag gcagaucggc cgaauuaagg auuggacgaa acggaaugac     2940

caccgccauc acgcgaugga ugcucucacc guggccuuca cuaaggacgu guuuauccag     3000

uauuucaaca auaagaacgc aucccuggau ccaaacgcca augagcaugc uauuaaaaac     3060

aaguacuuuc agaaugggag agccauagca cccaugccuc uuagggaguu ccgggcugag     3120

gccaaaaagc accuggaaaa cacauugauc ucaaucaaag cgaagaauaa ggucauuacc     3180

ggaaacauca auaaaacacg aaagaaaggu ggcguuaaca agaauaugca acagacucca     3240

agagggcagc uccaucugga gaccauuuac ggaagcggca aacaguaucu aacgaaggaa     3300

gagaaaguga acgccucuuu ugacaugcgc aagauaggua ccguguccaa gagugcuuac     3360

agggacgcac ugcuuaaacg ucucuaugag aaugauaacg accccaagaa agccuucgcu     3420

gggaagaaua gccuggauaa acaaccgauc ugguuggaca aggaacagau gcggaaggua     3480

ccugagaaag ucaagauugu gacacuggaa gccaucuaca cuauuagaaa agagaucucu     3540

cccgauuuaa agguugacaa agugauagau gucggcguga ggaagauccu cauugaccgc     3600

cugaacgagu auggaaauga ugcaaagaaa gcguuuucca accuugacga aaauccaauc     3660

uggcugaaca aggagaaagg cauuucaauc aagcgaguua ccauaagugg gauuagcaac     3720

gcccagucgu ugcacgugaa aaaggauaag gacggaaaac cuauccucga cgaaaauggc     3780

agaaacaucc ccguagauuu cgucaauaca gguaacaauc accauguggc uguguacuau     3840

cggccaguca uugacaagag ggggcagcug guuguggaug aggccggaaa ccccaaauac     3900

gaacuagagg agguaguggu cuccuucuuu gaagcaguga cucgcgcuaa ucugggccaa     3960

ccuauaaucg acaaggauua caaaaccacg gaggguuggc aguuccuuuu cucuaugaag     4020

cagaacgaau auuucguuuu uccaaaugag aaaacaggcu ucaacccgaa ggaaauugac     4080

cuccuggaug uggagaauua cggguugauc agccccaacu uauuucgugu ccaaaaguuc     4140

ucacugaaaa auuauguguu uagacaccau cucgagacca cuauuaagga cacaaguucc     4200

auccugcggg gaauaaccug gaucgauuuc aggucuagca aaggccuuga cacuauuguu     4260

aaggugcgcg ucaaccacau cgggcagauu guauccgugg gagaauacug a              4311


<210>  3359
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3359
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acaaagauga gagguccucu auagucaagu uggguguucg ggugaauccc      120

uuaacaguag acgaacugac uaacuucgag aaaggcaagu caaucacgac caaugcagau      180

cgcacacuaa aacgagggau gcguagaaac cuccagaggu acaagcugcg gcgcgaaacu      240

cuuaccgagg ugcugaagga gcacaaauug auuacagaag acaccauccu cagugagaau      300

ggaaacagaa cuacauuuga aaccuauagg cugcgggcca aggcggucac ggaggagauu      360

agccuggaag aguucgcucg cgugcucuua augaucaaua aaaagcgagg cuacaaaucc      420

ucuagaaagg ccaagggugu ugaagagggg acucugauag auggaaugga caucgcaagg      480

gaacuuuaua acaauaacuu gaccccuggc gagcugugcc uacaacuccu ggaugccggc      540

aaaaaguuuc uuccagacuu cuaccggucg gaucugcaga augaguugga ccguauuugg      600

gaaaaacaga aggaguauua cccggaaauc cucacagaug ugcugaaaga ggaauuacgc      660

gggaagaaaa gagacgcugu cugggcaauu ugugccaagu auuuugugug gaaggagaac      720

uacacugagu ggaauaaaga aaagggaaaa accgagcagc aagaaaggga gcauaagcug      780

gaagguaucu acucaaaacg gaagcgagau gaggcuaagc gcgagaaccu ccaguggaga      840

guaaacggcc uuaaagaaaa gcugagcuug gagcagcuag uuauaguguu ccaggaaaug      900

aauacacaaa uuaacaauag uuccggguau cugggagcca ucucugacag gagcaaagag      960

cucuacuuua acaagcagac cgucggccag uaucagaugg agaugcugga caaaaauccc     1020

aacgcguccc uucggaauau gguguucuac cgccaagauu aucuggacga auuuaacaug     1080

uugugggaga agcaggcagu guaccacaag gaacucacgg aggaacugaa aaaggagauu     1140

agagauauca ucauuuucua ucagaggcgu uuaaaaucac aaaagggucu gauaggguuu     1200

ugcgaguucg aaagucggca gaucgagguc gacauugaug gcaaaaagaa gaucaaaacu     1260

guuggaaauc gagugauaag cagaucuucc ccucuauuuc aggaguucaa gauuuggcag     1320

auccucaaca auaucgaggu aacagugguc ggcaaaaaga ggaaacgccg gaagcugaaa     1380

gaaaacuacu cagcccuuuu cgaggaguug aaugacgcug aacaacugga gcucaacggg     1440

agcagaaggc ugugucagga agagaaggaa cuucuggccc aggaguuguu uauucgcgau     1500

aagaugacca aaagugaggu gcucaagcug uuauucgaca auccacagga acuggaucua     1560

aacuuuaaaa cuaucgacgg aaacaagaca gguuacgcac ucuuccaagc auauucuaaa     1620

augauugaga uguccggcca ugaacccguu gauuuuaaga agccugugga gaaagucgug     1680

gaguacauaa aggccguauu cgaccugcuu aauuggaaca ccgacaucuu gggguuuaau     1740

agcaacgaag aacuggauaa ucagccauau uacaaacucu ggcaccuguu auauucguuc     1800

gaaggagaca acacgcccac cggcaauggu cgacugauuc agaagaugac agagcuuuac     1860

ggguuugaaa aagaguaugc gacuaucuug gccaacguuu cuuuccagga ugacuacggc     1920

ucccucucag caaaggcuau ccauaagauu cugccucacc uaaaagaggg aaaucgguac     1980

gauguggccu gcgucuaugc uggcuaccgu cacagugaaa gcucccugac cagagaggaa     2040

auagccaaca aggugcucaa agacaggcug augcuuuugc caaagaauag ucugcauaac     2100

ccggucgugg agaaaaucuu aaaucaaaug guuaacguga uuaauguaau cauugauauc     2160

uaugggaagc ccgacgaaau acgcgucgag cucgcacggg agcugaagaa aaacgccaag     2220

gaaagagagg aacuuacaaa aagcaucgcu cagacuacca aggcgcacga ggaauacaaa     2280

acacuguugc agaccgaguu uggacucacu aacgugucaa ggacggauau ucugcgauau     2340

aagcuauaca aagagcugga aaguuguggu uauaagacac ucuacuccaa uaccuacauc     2400

agccgcgaga agcuguucuc uaaagaauuu gacauugagc auaucauacc ucaggcacgg     2460

cuuuucgaug acucauucag uaacaagacu uuggaggcca gaagcguuaa uauugaaaaa     2520

ggcaacaaga ccgcuuauga uuuugugaaa gagaaguucg gggaauccgg agccgacaau     2580

ucucuggagc acuacuuaaa caauaucgaa gaccucuuua agucgggcaa aauuagcaag     2640

acaaaauaua acaagcugaa aauggcagag caagauaucc cagacgguuu cauagagagg     2700

gaucuucgua auacccagua caucgccaag aaggcucugu ccaugcuaaa cgaaauuuca     2760

caccgcgucg uggccacuuc ugggucugua acagacaaac uccgggagga uuggcagcug     2820

aucgacguga ugaaggaauu gaauugggag aaauauaagg cacugggcuu aguugaauac     2880

uuugaggaua gagacggaag gcaaauuggc cgaaucaaag auuggacgaa gcggaacgac     2940

caucgccacc augcgaugga ugcucucacc gucgccuuca cuaaggacgu guuuauacag     3000

uauuucaaua acaaaaaugc aagccuggau cccaacgcca acgagcacgc uauuaagaau     3060

aaauacuuuc agaacgggag agccaucgca ccuaugccac uuagggaguu ccgggcugaa     3120

gccaagaaac aucuggaaaa uacauugauc uccauuaagg cgaagaacaa agugaucacc     3180

ggaaauauua acaagacacg aaagaaaggu ggcgucaaua aaaacaugca gcaaacuccc     3240

agagggcagc uccaccugga gaccauauac ggaucaggca agcaguaucu aacgaaagaa     3300

gagaagguua augccagcuu ugacaugcgc aagaucggua ccgugucuaa auccgcuuac     3360

agggacgcac ugcuuaagcg ucucuaugag aacgauaaug acccgaaaaa ggccuucgcu     3420

gggaaaaaca gucuggauaa gcagccuauu ugguuggaca aggaacaaau gcggaaagug     3480

cccgagaagg uaaaaaucgu cacacuggaa gccauuuaca cuaucagaaa ggagauaagc     3540

ccagauuuaa aaguggacaa gguuaucgau gugggcguca ggaagauucu caucgaccgc     3600

cugaaugagu auggaaacga ugcuaaaaag gcguuuucua aucuugacaa aaacccuauu     3660

uggcugaaua aggaaaaagg caucuccaua aagcgaguga ccauuucagg gaucaguaac     3720

gcccagagcu ugcacguuaa gaaagauaag gacggaaaac ccauccucga cgagaacggc     3780

agaaauauuc caguggauuu cguaaacaca gguaauaacc aucacgucgc uguguacuau     3840

cggcccguga uagacaagag ggggcagcug gucguugaug aagccggaaa uccuaaauac     3900

gagcuagaag aggugguagu gucguucuuu gaggcaguca cucgcgcuaa ccugggccuu     3960

ccaaucauug acaaggauua caaaaccacg gaagguuggc aguuccucuu uuccaugaag     4020

caaaaugagu auuucguguu uccgaacgaa aagacaggcu ucaaucccaa agagaucgac     4080

cuguuggaug uugaaaacua cggguuaauu ucuccuaauc uguuucgugu gcagaaguuc     4140

agccucaaaa acuaugucuu uagacaucac cuggagacca cuaucaagga cacaucaagu     4200

auacuucggg gaaucaccug gauugauuuc agguccucua aaggccugga cacuaucgug     4260

aagguucgcg ugaaucacau ugggcagauc gucagcguag gagaguacug a              4311


<210>  3360
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3360
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acaaagauga gagguccucu auagucaagu uggguguucg ggugaauccc      120

uuaacaguag acgaacugac uaacuucgag aaaggcaagu caaucacgac caaugcagau      180

cgcacacuaa aacgagggau gcguagaaac cuccagaggu acaagcugcg gcgcgaaacu      240

cuuaccgagg ugcugaagga gcacaaauug auuacagaag acaccauccu cagugagaau      300

ggaaacagaa cuacauuuga aaccuauagg cugcgggcca aggcggucac ggaggagauu      360

agccuggaag aguucgcucg cgugcucuua augaucaaua aaaagcgagg cuacaaaucc      420

ucuagaaagg ccaagggugu ugaagagggg acucugauag auggaaugga caucgcaagg      480

gaacuuuaua acaauaacuu gaccccuggc gagcugugcc uacaacuccu ggaugccggc      540

aaaaaguuuc uuccagacuu cuaccggucg gaucugcaga augaguugga ccguauuugg      600

gaaaaacaga aggaguauua cccggaaauc cucacagaug ugcugaaaga ggaauuacgc      660

gggaagaaaa gagacgcugu cugggcaauu ugugccaagu auuuugugug gaaggagaac      720

uacacugagu ggaauaaaga aaagggaaaa accgagcagc aagaaaggga gcauaagcug      780

gaagguaucu acucaaaacg gaagcgagau gaggcuaagc gcgagaaccu ccaguggaga      840

guaaacggcc uuaaagaaaa gcugagcuug gagcagcuag uuauaguguu ccaggaaaug      900

aauacacaaa uuaacaauag uuccggguau cugggagcca ucucugacag gagcaaagag      960

cucuacuuua acaagcagac cgucggccag uaucagaugg agaugcugga caaaaauccc     1020

aacgcguccc uucggaauau gguguucuac cgccaagauu aucuggacga auuuaacaug     1080

uugugggaga agcaggcagu guaccacaag gaacucacgg aggaacugaa aaaggagauu     1140

agagauauca ucauuuucua ucagaggcgu uuaaaaucac aaaagggucu gauaggguuu     1200

ugcgaguucg aaagucggca gaucgagguc gacauugaug gcaaaaagaa gaucaaaacu     1260

guuggaaauc gagugauaag cagaucuucc ccucuauuuc aggaguucaa gauuuggcag     1320

auccucaaca auaucgaggu aacagugguc ggcaaaaaga ggaaacgccg gaagcugaaa     1380

gaaaacuacu cagcccuuuu cgaggaguug aaugacgcug aacaacugga gcucaacggg     1440

agcagaaggc ugugucagga agagaaggaa cuucuggccc aggaguuguu uauucgcgau     1500

aagaugacca aaagugaggu gcucaagcug uuauucgaca auccacagga acuggaucua     1560

aacuuuaaaa cuaucgacgg aaacaagaca gguuacgcac ucuuccaagc auauucuaaa     1620

augauugaga uguccggcca ugaacccguu gauuuuaaga agccugugga gaaagucgug     1680

gaguacauaa aggccguauu cgaccugcuu aauuggaaca ccgacaucuu gggguuuaau     1740

agcaacgaag aacuggauaa ucagucguau uacaaacucu ggcaccuguu auauucuuuc     1800

gaaggagaca acacgccaac cggcaauggu cgacugauuc agaagaugac agagcuuuac     1860

ggguuugaaa aagaguaugc gacuaucuug gccaacguuu ccuuccagga ugacuacggc     1920

ucacucagug caaaggcuau ccauaagauu cugccccacc uaaaagaggg aaaucgguac     1980

gauguggccu gcgucuaugc uggcuaccgu cacagcgaau ccucucugac cagagaggaa     2040

auagccaaca aggugcucaa agacaggcug augcuuuugc cuaagaauag ccugcauaac     2100

ccagucgugg agaaaaucuu aaaucaaaug guuaacguga uuaauguaau cauugauauc     2160

uaugggaagc cggacgaaau acgcgucgag cucgcacggg agcugaagaa aaacgccaag     2220

gaaagagagg aacuuacaaa aucaaucgcu cagacuacca aggcgcacga ggaauacaaa     2280

acacuguugc agaccgaguu uggacucacu aacgugagua ggacggauau ucugcgauau     2340

aagcuauaca aagagcugga auccuguggu uauaagacac ucuacagcaa uaccuacauc     2400

ucucgcgaga agcuguucuc aaaagaauuu gacauugagc auaucauacc ccaggcacgg     2460

cuuuucgaug acaguuucag caacaagacu uuggaggcca gauccguuaa uauugaaaaa     2520

ggcaacaaga ccgcuuauga uuuugugaaa gagaaguucg gggaaucugg agccgacaau     2580

ucgcuggagc acuacuuaaa caauaucgaa gaccucuuua agagcggcaa aauuuccaag     2640

acaaaauaua acaagcugaa aauggcagag caagauaucc cugacgguuu cauagagagg     2700

gaucuucgua auacccagua caucgccaag aaggcucugu caaugcuaaa cgaaauuagu     2760

caccgcgucg uggccacuuc ugggagcgua acagacaaac uccgggagga uuggcagcug     2820

aucgacguga ugaaggaauu gaauugggag aaauauaagg cacugggcuu aguugaauac     2880

uuugaggaua gagacggaag gcaaauuggc cgaaucaaag auuggacgaa gcggaacgac     2940

caucgccacc augcgaugga ugcucucacc gucgccuuca cuaaggacgu guuuauacag     3000

uauuucaaua acaaaaaugc aucccuggau ccaaacgcca acgagcacgc uauuaagaau     3060

aaauacuuuc agaacgggag agccaucgca cccaugccuc uuagggaguu ccgggcugaa     3120

gccaagaaac aucuggaaaa uacauugauc ucaauuaagg cgaagaacaa agugaucacc     3180

ggaaauauua acaagacacg aaagaaaggu ggcgucaaua aaaacaugca gcaaacucca     3240

agagggcagc uccaccugga gaccauauac ggaagcggca agcaguaucu aacgaaagaa     3300

gagaagguua augccucuuu ugacaugcgc aagaucggua ccguguccaa acuggcuuac     3360

agggacgcac uucucaagcg ucuguaugag aacgauaaug accccaaaaa ggccuucgcu     3420

gggaaaaaca guuuggauaa gcagccgauu uggcuggaca aggaacaaau gcggaaagug     3480

ccugagaagg uaaaaaucgu cacauuagaa gccauuuaca cuaucagaaa ggagauaagc     3540

cccgaucuca aaguggacaa gguuaucgau gugggcguca ggaagauucu gaucgaccgc     3600

cuuaaugagu auggaaacga ugcuaaaaag gcguuuucua aucuggacga aaacccaauu     3660

ugguugaaua aagagaaggg caucuccaua aaacgaguga ccauuucagg gaucaguaac     3720

gcccagagcc uccacguuaa gaaggauaaa gacggaaagc cuauccugga cgaaaacggc     3780

agaaauauuc ccguggauuu cguaaacaca gguaauaacc aucacgucgc uguguacuau     3840

cggccaguga uagacaaaag ggggcagcua gucguugaug aggccggaaa ucccaaguac     3900

gaacuggagg aggugguagu gucguucuuu gaagcaguca cucgcgcuaa ccuuggccag     3960

ccuaucauug acaaagauua caagaccacg gaggguuggc aauuccucuu uuccaugaaa     4020

cagaaugaau auuucguguu uccaaacgag aagacaggcu ucaauccgaa ggaaaucgac     4080

cuguuggaug uugagaacua cggguuaauu ucucccaauc uguuucgugu gcagaaauuc     4140

agccucaaga acuaugucuu uagacaucac cuggagacca cuaucaaaga cacaucaagu     4200

auacuucggg gaaucaccug gauugauuuc agguccucua agggccugga cacuaucgug     4260

aaaguucgcg ugaaucacau ugggcaaauc gucagcguag gagaauacug a              4311


<210>  3361
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3361
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acaaagauga gagguccucu auagucaagu uggguguucg ggugaauccc      120

uuaacaguag acgaacugac uaacuucgag aaaggcaagu caaucacgac caaugcagau      180

cgcacacuaa aacgagggau gcguagaaac cuccagaggu acaagcugcg gcgcgaaacu      240

cuuaccgagg ugcugaagga gcacaaauug auuacagaag acaccauccu cagugagaau      300

ggaaacagaa cuacauuuga aaccuauagg cugcgggcca aggcggucac ggaggagauu      360

agccuggaag aguucgcucg cgugcucuua augaucaaua aaaagcgagg cuacaaaucc      420

ucuagaaagg ccaagggugu ugaagagggg acucugauag auggaaugga caucgcaagg      480

gaacuuuaua acaauaacuu gaccccuggc gagcugugcc uacaacuccu ggaugccggc      540

aaaaaguuuc uuccagacuu cuaccggucg gaucugcaga augaguugga ccguauuugg      600

gaaaaacaga aggaguauua cccggaaauc cucacagaug ugcugaaaga ggaauuacgc      660

gggaagaaaa gagacgcugu cugggcaauu ugugccaagu auuuugugug gaaggagacu      720

uacaccgagu ggaacaaaga aaagggaaaa acagagcagc aagaaaggga gcauaagcug      780

gaagguaucu acucaaaacg gaagcgagau gaggcuaagc gcgagaaucu ccaguggaga      840

guaaacggcc uuaaagaaaa gcugagcuug gagcagcuag uuauaguguu ccaggaaaug      900

aacacccaaa uuaauaacag uuccggguau cugggagcca ucucugacag gagcaaagag      960

cucuacuuua auaagcagac ggucggccag uaucagaugg agaugcugga caaaaacccc     1020

aaugcguccc uucggaacau gguguucuac cgccaagauu aucuggacga auuuaauaug     1080

uugugggaga agcaggcagu guaccacaag gaacucacug aggaacugaa aaaggagauu     1140

agagauauca ucauuuucua ucagaggcgu uuaaaaucac aaaagggucu gauaggguuu     1200

ugcgaguucg aaagucggca gaucgagguc gacauugaug gaaagaagaa gaucaaaaca     1260

guuggcaacc gagugauaag cagaucuucc ccucuauuuc aggaguucaa gauuuggcag     1320

auccucaaua acaucgaagu aaccgugguc ggcaaaaaga ggaaacgccg gaagcugaaa     1380

gaaaauuacu cagccauuuu cgaggagcuu aacgacgcug aacaauugga gcugaauggg     1440

agcagaaggc ucugucagga agagaaggaa cugcuugccc aggagcuguu ugugcgcgau     1500

aagaugacua aaagugaggu uuugaagcuc cuguucgaca acccacagga auuagaucug     1560

aauuuuaaaa caaucgacgg aaacaagacc gguuacgcac uauuccaggc uuauucuaaa     1620

augauugaga uguccggcca ugaacccgug gauuuuaaga agccugucga gaaaguggua     1680

gaguacauaa aggccguuuu cgaccuccug aacuggaaua cggacauccu uggguuuaac     1740

agcaaugaag aguuggauaa ccaaucguau uacaaacugu ggcaccuccu guauucuuuc     1800

gaaggagaca auaccccaac aggcaacggu cgauuaauuc agaagaugac ugagcuguac     1860

ggguuugaaa aagaguaugc gaccauccuu gccaaugugu ccuuccagga ugacuacggc     1920

ucauugagug caaaggcuau ccauaagauu cucccccacc ugaaagaggg aaaccgguac     1980

gaugucgccu gcguguaugc uggcuaccgu cacagcgaau ccucucuaac aagagaggaa     2040

auagccaaua agguccugaa agacaggcuc augcugcuuc cuaagaacag cuugcauaau     2100

ccagugguug agaaaauccu gaaccaaaug gugaauguaa uuaacgucau cauugauauc     2160

uaugggaagc cggacgaaau acgcguggag uuagcacggg agcucaagaa aaaugccaag     2220

gaaagagagg aacugacuaa aucaaucgcu cagaccacaa aggcgaacga ggaauacaaa     2280

acccuucugc agacugaguu uggauugacg aacguuagua ggacagauau ucuccgauau     2340

aagcuguaca aagagcuaga auccuguggu uauaagaccc uguacagcaa uacuuacauc     2400

ucucgcgaga aacucuucuc aaaagaauuu gacauugagc acaucauacc ccaggcacgg     2460

cuguucgaug acaguuucag caacaagacc cuugaggcca gauccgugaa uauugaaaaa     2520

ggcaacaaga cagcuuauga uuuugucaaa gagaaguucg gggaaucugg agccgacaau     2580

ucguuggagc auuaccugaa caauaucgaa gacuuauuua agagcggcaa aauuuccaag     2640

accaaauaua acaagcucaa aauggcaaag caagauaucc cugacgguuu cauagagagg     2700

gaucugcgua auacucagua caucgccaag aaagcucuuu caaugcugaa cgagauuagu     2760

caccgcgugg uagccacauc ugggagcgug acggacaagc uacgggaaga uuggcagcuc     2820

aucgacguua ugaaagagcu gaauugggaa aaguauaaag cauugggccu ggucgaguac     2880

uuugaagaua gagacggaag gcaaauuggc cgaaucaagg auuggaccaa gcggaacgac     2940

caccgccauc acgcgaugga ugcuuuaacu guggccuuca caaaagacgu guuuauacag     3000

uauuucaaua acaagaaugc aucccucgau ccaaacgcca acgagcaugc uauuaaaaau     3060

aaguacuuuc agaacgggag agccaucgca cccaugccuc ugagggaguu ccgggcugaa     3120

gccaaaaagc accuugagaa uacccugauc ucaauuaagg cgaaaaacaa ggucaucaca     3180

ggaaauauua acaaaacucg aaagaaaggu ggcguuaaua agaacaugca gcaaacccca     3240

agagggcagu ugcaucucga aacgauauac ggaagcggca aacaguaucu gaccaaggag     3300

gaacgcguga augccucuuu ugacaugagg aagaucgaga caguguccaa aagugcuuac     3360

cgugacgcac uacugaagcg gcuuuaugag aacgauaaug accccaaaaa ggccuucgcu     3420

gguaaaaaca gccucgauaa gcagccgauu uggcuggaca aggaacagaa aagaaaggua     3480

ccugagaaag ucaagaucgu gacuuuggaa gccauuuaca ccaucaggaa agagauaucu     3540

cccgaucuga agguugacaa ggugaucgau gucggggugc gcaaaauuuu aaucgaccga     3600

cucaaugagu auggcaacga ugcaaagaaa gcguuuucca aucuggacga aaacccaauu     3660

uggcuuaaua aggagaaagg aaucucaaua aagagaguua caauuagugg caucagcaac     3720

gcccaaucgc ugcacgugaa gaaagauaag gacgggaaac cuaucuugga cgaaaacgga     3780

cggaauauuc ccguagauuu cgucaacacu ggcaauaacc accauguggc uguguacuau     3840

aggccaguca uagacaagcg cggucagcuc guuguggaug aggccgggaa ucccaaauac     3900

gaacuggagg agguaguggu cuccuucuuu gaagcaguga cccgugcuaa ccuaggacag     3960

ccuaucauug acaaggauua caaaacgaca gagggcuggc aauuccuguu uucuaugaag     4020

cagaaugaau auuucguuuu uccaaacgag aagaccgguu ucaauccgaa agaaaucgac     4080

cuucucgaug uggagaacua cggccugauu agccccaauu uguuuagagu ccagaaguuc     4140

ucacugaaaa acuauguguu ucggcaccau uuagagacua caaucaagga caccaguucc     4200

auacucaggg ggaucacuug gauugauuuc cgcucuagca aaggacugga caccaucguu     4260

aaggugcgag ucaaucacau uggccaaauc guauccgugg gggaauacug a              4311


<210>  3362
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3362
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acaaagauga gagguccucu auagucaagu uggguguucg ggugaauccc      120

uuaacaguag acgaacugac uaacuucgag aaaggcaagu caaucacgac caaugcagau      180

cgcacacuaa aacgagggau gcguagaaac cuccagaggu acaagcugcg gcgcgaaacu      240

cuuaccgagg ugcugaagga gcacaaauug auuacagaag acaccauccu cagugagaau      300

ggaaacagaa cuacauuuga aaccuauagg cugcgggcca aggcggucac ggaggagauu      360

agccuggaag aguucgcucg cgugcucuua augaucaaua aaaagcgagg cuacaaaucc      420

ucuagaaagg ccaagggugu ugaagagggg acucugauag auggaaugga caucgcaagg      480

gaacuuuaua acaauaacuu gaccccuggc gagcugugcc uacaacuccu ggaugccggc      540

aaaaaguuuc uuccagacuu cuaccggucg gaucugcaga augaguugga ccguauuugg      600

gaaaaacaga aggaguauua cccggaaauc cucacagaug ugcugaaaga ggaauuacgc      660

gggaagaaaa gagacgcugu cugggcaauu ugugccaagu auuuugugug gaaggagacu      720

uacaccgagu ggaacaaaga aaagggaaaa acagagcagc aagaaaagga gcauaaacug      780

gaagguaucu acucaaagag gaagcgggau gaggcuaaac gagagaaucu ccaguggcgc      840

guaaacggcc uuaaggaaaa acugagcuug gagcagcuag uuauaguguu ccaggaaaug      900

aacacccaaa uuaauaacag uuccggguau cugggagcca ucucugacag aagcaaggag      960

cucuacuuua auaaacagac ggucggccag uaucagaugg agaugcugga caagaacccc     1020

aaugcguccc uuaggaacau gguguucuac cggcaagauu aucuggacga auuuaauaug     1080

uugugggaga agcaggcagu guaccacaaa gaacucacug aggaacugaa gaaagagauu     1140

cgcgauauca ucauuuucua ucagagaagg uuaaagucac aaaaaggucu gauaggguuu     1200

ugcgaguucg aaagucguca gaucgagguc gacauugaug gaaagaagaa aaucaagaca     1260

guuggcaacc gggugauaag ccgaucuucc ccucuauuuc aggaguucaa aauuuggcag     1320

auccucaaua acaucgaggu aaccgugguc ggcaagaaaa gaaagaggcg caaacugaag     1380

gaaaauuacu cagcccuuuu cgaggaguug aacgacgcug aacaacugga gcucaauggg     1440

agccggagac ugugucagga agagaaggaa cuucuggccc aggaguuguu uauuagggau     1500

aaaaugacua agagugaggu gcucaaacug uuauucgaca acccacagga acuggaucua     1560

aauuuuaaga caaucgacgg aaacaaaacc gguuacgcac ucuuccaggc uuauucuaag     1620

augauugaga uguccggcca ugaacccguu gauuuuaaga aaccugugga gaaggucgug     1680

gaguacauaa aagccguauu cgaccugcuu aacuggaaua cggacaucuu gggguuuaac     1740

agcaaugaag aacuggauaa ccaaucguau uacaagcucu ggcaccuguu auauucuuuc     1800

gaaggagaca auaccccaac aggcaacggu cgccugauuc agaaaaugac ugagcuuuac     1860

ggguuugaaa aggaguaugc gaccaucuug gccaauguuu ccuuccagga ugacuacggc     1920

ucacucagug caaaggcuau ccauaaaauu cugccccacc uaaaggaggg aaaccgauac     1980

gauguggccu gcgucuaugc uggcuaccgg cacagcgaau ccucucugac acgugaggaa     2040

auagccaaua aagugcucaa ggacagacug augcuuuugc cuaaaaacag ccugcauaau     2100

ccagucgugg agaagaucuu aaaccaaaug guuaauguga uuaacguaau cauugauauc     2160

uaugggaagc cggacgaaau aagggucgag cucgcacgcg agcugaaaaa gaaugccaaa     2220

gaacgggagg aacuuacuaa gucaaucgcu cagaccacaa aagcgcacga ggaauacaag     2280

acccuguugc agacugaguu uggacucacg aacgugagua gaacagauau ucugagguau     2340

aaacuauaca aggagcugga auccuguggu uauaagaccc ucuacagcaa cacuuacauc     2400

ucucgagaga aacuguucuc aaaggaauuu gacauugagc auaucauacc ccaggcacgc     2460

cuuuucgaug acaguuucag caauaaaacc uuggaggccc gguccguuaa cauugaaaag     2520

ggcaauaaaa cagcuuauga uuuugugaag gagaaguucg gggaaucugg agccgacaac     2580

ucgcuggagc acuacuuaaa uaacaucgaa gaccucuuua aaagcggcaa gauuuccaaa     2640

accaaguaua auaaacugaa gauggcagag caagauaucc cugacgguuu cauagagaga     2700

gaucuuagga acacucagua caucgccaag aaagcucugu caaugcuaaa ugaaauuagu     2760

caccgugucg uggccacauc ugggagcgua acggacaagc uccgcgagga uuggcagcug     2820

aucgacguga ugaaagaauu gaacugggag aaguauaaag cacugggcuu aguugaauac     2880

uuugaggauc gggacggaag acaaauuggc aggaucaagg auuggaccaa gcgaaaugac     2940

caucggcacc augcgaugga ugcucucacu gucgccuuca caaaagacgu guuuauacag     3000

uauuucaaca auaagaacgc aucccuggau ccaaaugcca acgagcacgc uauuaaaaac     3060

aaguacuuuc agaaugggcg cgccaucgca cccaugccuc uuagagaguu cagggcugaa     3120

gccaaaaagc aucuggaaaa caccuugauc ucaauuaagg cgaaaaauaa ggugaucaca     3180

ggaaacauua auaaaacucg gaagaaaggu ggcgucaaca agaauaugca gcaaacccca     3240

cgagggcagc uccaccugga gacgauauac ggaagcggca aacaguaucu aaccaaggaa     3300

gagaagguua acgccucuuu ugacaugaga aaaaucggua caguguccaa gcuggcuuac     3360

cgcgacgcac uucucaaaag gcuguaugag aaugauaacg accccaagaa agccuucgcu     3420

gggaagaaua guuuggauaa gcagccgauu uggcuggaca aagaacaaau gcguaaggug     3480

ccugagaaag uaaagaucgu cacuuuagaa gccauuuaca ccauccggaa agagauaagc     3540

cccgaucuca agguggacaa gguuaucgau gugggcguca gaaaaauucu gaucgacagg     3600

cuuaacgagu auggaaauga ugcaaagaaa gcguuuucua accuggacga aaauccaauu     3660

ugguugaaca aggagaaagg caucuccaua aagcgcguga caauuucagg gaucaguaau     3720

gcccagagcc uccacguuaa gaaagauaag gacggaaaac cuauccugga cgaaaacggc     3780

cgaaacauuc ccguggauuu cguaaauacu gguaacaauc aucacgucgc uguguacuau     3840

agaccaguga uagacaagcg ggggcagcua gucguugaug aggccggaaa ccccaaauac     3900

gaacuggagg aggugguagu gucguucuuu gaagcaguca ccagggcuaa ucuuggccag     3960

ccuaucauug acaaggauua caaaacgaca gaggguuggc aauuccucuu uuccaugaag     4020

cagaacgaau auuucguguu uccaaaugag aagaccggcu ucaacccgaa agaaaucgac     4080

cuguuggaug uugagaauua cggguuaauu ucucccaacc uguuucgcgu gcagaaguuc     4140

agccucaaaa auuaugucuu ucgucaucac cuggagacua caaucaagga caccucaagu     4200

auacuuagag gaaucacuug gauugauuuc cgguccucua aaggccugga caccaucgug     4260

aagguuaggg ugaaccacau ugggcaaauc gucagcguag gagaauacug a              4311


<210>  3363
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3363
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acaaagauga gagguccucu auagucaagu uggguguucg ggugaauccc      120

uuaacaguag acgaacugac uaacuucgag aaaggcaagu caaucacgac caaugcagau      180

cgcacacuaa aacgagggau gcguagaaac cuccagaggu acaagcugcg gcgcgaaacu      240

cuuaccgagg ugcugaagga gcacaaauug auuacagaag acaccauccu cagugagaau      300

ggaaacagaa cuacauuuga aaccuauagg cugcgggcca aggcggucac ggaggagauu      360

agccuggaag aguucgcucg cgugcucuua augaucaaua aaaagcgagg cuacaaaucc      420

ucuagaaagg ccaagggugu ugaagagggg acucugauag auggaaugga caucgcaagg      480

gaacuuuaua acaauaacuu gaccccuggc gagcugugcc uacaacuccu ggaugccggc      540

aaaaaguuuc uuccagacuu cuaccggucg gaucugcaga augaguugga ccguauuugg      600

gaaaaacaga aggaguauua cccggaaauc cucacagaug ugcugaaaga ggaauuacgc      660

gggaagaaaa gagacgcugu cugggcaauu ugugccaagu auuuugugug gaaggagaac      720

uacacugagu ggaauaaaga aaagggaaaa accgagcagc aagaaaggga gcauaagcug      780

gaagguaucu acucaaaacg gaagcgagau gaggcuaagc gcgagaaccu ccaguggaga      840

guaaacggcc uuaaagaaaa gcugagcuug gagcagcuag uuauaguguu ccaggaaaug      900

aauacacaaa uuaacaauag uuccggguau cugggagcca ucucugacag gagcaaagag      960

cucuacuuua acaagcagac cgucggccag uaucagaugg agaugcugga caaaaauccc     1020

aacgcguccc uucggaauau gguguucuac cgccaagauu aucuggacga auuuaacaug     1080

uugugggaga agcaggcagu guaccacaag gaacucacgg aggaacugaa aaaggagauu     1140

agagauauca ucauuuucua ucagaggcgu uuaaaaucac aaaagggucu gauaggguuu     1200

ugcgaguucg aaagucggca gaucgagguc gacauugaug gcaaaaagaa gaucaaaacu     1260

guuggaaauc gagugauaag cagaucuucc ccucuauuuc aggaguucaa gauuuggcag     1320

auccucaaca auaucgaggu aacagugguc ggcaaaaaga ggaaacgccg gaagcugaaa     1380

gaaaacuacu cagcccuuuu cgaggaguug aaugacgcug aacaacugga gcucaacggg     1440

agcagaaggc ugugucagga agagaaggaa cuucuggccc aggaguuguu uauucgcgau     1500

aagaugacca aaagugaggu gcucaagcug uuauucgaca auccacagga acuggaucua     1560

aacuuuaaaa cuaucgacgg aaacaagaca gguuacgcac ucuuccaagc auauucuaaa     1620

augauugaga uguccggcca ugaacccguu gauuuuaaga agccugugga gaaagucgug     1680

gaguacauaa aggccguauu cgaccugcuu aauuggaaca ccgacaucuu gggguuuaau     1740

agcaacgaag aacuggauaa ucagccauau uacaaacucu ggcaccuguu auauucguuc     1800

gaaggagaca acacgcccac cggcaauggu cgacugauuc agaagaugac agagcuuuac     1860

ggguuugaaa aagaguaugc gacuaucuug gccaacguuu cuuuccagga ugacuacggc     1920

ucccucucag caaaggcuau ccauaagauu cugccucacc uaaaagaggg aaaucgguac     1980

gauguggccu gcgucuaugc uggcuaccgu cacagugaaa gcucccugac cagagaggaa     2040

auagccaaca aggugcucaa agacaggcug augcuuuugc caaagaauag ucugcauaac     2100

ccggucgugg agaaaaucuu aaaucaaaug guuaacguga uuaauguaau cauugauauc     2160

uaugggaagc ccgacgaaau acgcgucgag cucgcacggg agcugaagaa aaacgccaag     2220

gaaagagagg aacuuacaaa aagcaucgcu cagacuacca aggcgcacga ggaauacaaa     2280

acacuguugc agaccgaguu uggacucacu aacgugucaa ggacggauau ucugcgauau     2340

aagcuauaca aagagcugga aaguuguggu uauaagacac ucuacuccaa uaccuacauc     2400

agccgcgaga agcuguucuc uaaagaauuu gacauugagc auaucauacc ucaggcacgg     2460

cuuuucgaug acucauucag uaacaagacu uuggaggcca gaagcguuaa uauugaaaaa     2520

ggcaacaaga ccgcuuauga uuuugugaaa gagaaguucg gggaauccgg agccgacaau     2580

ucucuggagc acuacuuaaa caauaucgaa gaccucuuua agucgggcaa aauuagcaag     2640

acaaaauaua acaagcugaa aauggcagag caagauaucc cagacgguuu cauagagagg     2700

gaucuucgua auacccagua caucgccaag aaggcucugu ccaugcuaaa cgaaauuuca     2760

caccgcgucg uggccacuuc ugggucugua acagacaaac uccgggagga uuggcagcug     2820

aucgacguga ugaaggaauu gaauugggag aaauauaagg cacugggcuu aguugaauac     2880

uuugaggaua gagacggaag gcaaauuggc cgaaucaaag auuggacgaa gcggaacgac     2940

caucgccacc augcgaugga ugcucucacc gucgccuuca cuaaggacgu guuuauacag     3000

uauuucaaua acaaaaaugc aagccuggau cccaacgcca acgagcacgc uauuaagaau     3060

aaauacuuuc agaacgggag agccaucgca ccuaugccac uuagggaguu ccgggcugaa     3120

gccaagaaac aucuggaaaa uacauugauc uccauuaagg cgaagaacaa agugaucacc     3180

ggaaauauua acaagacacg aaagaaaggu ggcgucaaua aaaacaugca gcaaacuccc     3240

agagggcagc uccaccugga gaccauauac ggaucaggca agcaguaucu aacgaaagaa     3300

gagaagguua augccagcuu ugacaugcgc aagaucggua ccgugucuaa auccgcuuac     3360

agggacgcac ugcuuaagcg ucucuaugag aacgauaaug acccgaaaaa ggccuucgcu     3420

gggaaaaaca gucuggauaa gcagccuauu ugguuggaca aggaacaaau gcggaaagug     3480

cccgagaagg uaaaaaucgu cacacuggaa gccauuuaca cuaucagaaa ggagauaagc     3540

ucugauuuaa aaguggacaa gguuaucgau gugggcguca ggaagauucu caucgaccgc     3600

cugaaugagu auggaaacga ugcuaaaaag gcguuuucca aucuugacaa aaacccaauu     3660

uggcugaaua aggaaaaagg caucucaaua aagcgaguga ccauuagugg gaucagcaac     3720

gcccagucgu ugcacguuaa gaaagauaag gacggaaaac cuauccucga cgagaacggc     3780

agaaauauuc ccguggauuu cguaaacaca gguaauaacc aucacgucgc uguguacuau     3840

cggccaguga uagacaagag ggggcagcug gucguugaug aagccggaaa ucccaaauac     3900

gagcuagaag aggugguagu guccuucuuu gaggcaguca cucgcgcuaa ccugggccuu     3960

ccuaucauug acaaggauua caaaaccacg gaagguuggc aguuccucuu uucuaugaag     4020

caaaaugagu auuucguguu uccaaacgaa aagacaggcu ucaauccgaa agagaucgac     4080

cuguuggaug uugaaaacua cggguuaauu agccccaauc uguuucgugu gcagaaguuc     4140

ucacucaaaa acuaugucuu uagacaucac cuggagacca cuaucaagga cacaaguucc     4200

auacuucggg gaaucaccug gauugauuuc aggucuagca aaggccugga cacuaucgug     4260

aagguucgcg ugaaucacau ugggcagauc gucuccguag gagaguacug a              4311


<210>  3364
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3364
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acaaagauga gagguccucu auagucaagu uggguguucg ggugaauccc      120

uuaacaguag acgaacugac uaacuucgag aaaggcaagu caaucacgac caaugcagau      180

cgcacacuaa aacgagggau gcguagaaac cuccagaggu acaagcugcg gcgcgaaacu      240

cuuaccgagg ugcugaagga gcacaaauug auuacagaag acaccauccu cagugagaau      300

ggaaacagaa cuacauuuga aaccuauagg cugcgggcca aggcggucac ggaggagauu      360

agccuggaag aguucgcucg cgugcucuua augaucaaua aaaagcgagg cuacaaaucc      420

ucuagaaagg ccaagggugu ugaagagggg acucugauag auggaaugga caucgcaagg      480

gaacuuuaua acaauaacuu gaccccuggc gagcugugcc uacaacuccu ggaugccggc      540

aaaaaguuuc uuccagacuu cuaccggucg gaucugcaga augaguugga ccguauuugg      600

gaaaaacaga aggaguauua cccggaaauc cucacagaug ugcugaaaga ggaauuacgc      660

gggaagaaaa gagacgcugu cugggcaauu ugugccaagu auuuugugug gaaggagaac      720

uacacugagu ggaauaaaga aaagggaaaa accgagcagc aagaaaggga gcauaagcug      780

gaagguaucu acucaaaacg gaagcgagau gaggcuaagc gcgagaaccu ccaguggaga      840

guaaacggcc uuaaagaaaa gcugagcuug gagcagcuag uuauaguguu ccaggaaaug      900

aauacacaaa uuaacaauag uuccggguau cugggagcca ucucugacag gagcaaagag      960

cucuacuuua acaagcagac cgucggccag ugccagaugg agaugcugga caaaaauccc     1020

aacgcguccc uucggaauau gguguucuau cgccaagauu accuggacga auuuaacaug     1080

uugugggaga agcaggcagu guaucacaag gaacucacgg aggaacugaa aaaggagauu     1140

agagauauca ucauuuucua ccagaggcgu uuaaaaucac aaaagggucu gauaggguuu     1200

ugugaguucg aaagucggca gaucgagguc gacauugaug gcaaaaagaa gaucaaaacu     1260

guuggaaauc gagugauaag cagaucuucc ccucuauuuc aggaguucaa gauuuggcag     1320

auccucaaca auaucgaggu aacagugguc ggcaaaaaga ggaaacgccg gaagcugaaa     1380

gaaaacuauu cagcccuuuu cgaggaguug aaugacgcug aacaacugga gcucaacggg     1440

agcagaaggc ugugccagga agagaaggaa cuucuggccc aggaguuguu uauucgcgau     1500

aagaugacca aaagugaggu gcucaagcug uuauucgaca auccacagga acuggaucua     1560

aacuuuaaaa cuaucgacgg aaacaagaca gguuacgcac ucuuccaagc auacucuaaa     1620

augauugaga uguccggcca ugaacccguu gauuuuaaga agccugugga gaaagucgug     1680

gaguauauaa aggccguauu cgaccugcuu aauuggaaca ccgacaucuu gggguuuaau     1740

agcaacgaag aacuggauaa ucagccauac uauaaacucu ggcaccuguu auacucguuc     1800

gaaggagaca acacgcccac cggcaauggu cgacugauuc agaagaugac agagcuuuau     1860

ggguuugaaa aagaguacgc gacuaucuug gccaacguuu cuuuccagga ugacuauggc     1920

ucccucucag caaaggcuau ccauaagauu cugccucacc uaaaagaggg aaaucgguac     1980

gauguggccu gugucuacgc uggcuaucgu cacagugaaa gcucccugac cagagaggaa     2040

auagccaaca aggugcucaa agacaggcug augcuuuugc caaagaauag ucugcauaac     2100

ccggucgugg agaaaaucuu aaaucaaaug guuaacguga uuaauguaau cauugauauc     2160

uacgggaagc ccgacgaaau acgcgucgag cucgcacggg agcugaagaa aaacgccaag     2220

gaaagagagg aacuuacaaa aagcaucgcu cagacuacca aggcgcacga ggaauauaaa     2280

acacuguugc agaccgaguu uggacucacu aacgugucaa ggacggauau ucugcgauac     2340

aagcuauaua aagagcugga aaguugcggu uacaagacac ucuauuccaa uaccuacauc     2400

agccgcgaga agcuguucuc uaaagaauuu gacauugagc auaucauacc ucaggcacgg     2460

cuuuucgaug acucauucag uaacaagacu uuggaggcca gaagcguuaa uauugaaaaa     2520

ggcaacaaga ccgcuuacga uuuugugaaa gagaaguucg gggaauccgg agccgacaau     2580

ucucuggagc acuauuuaaa caauaucgaa gaccucuuua agucgggcaa aauuagcaag     2640

acaaaauaca acaagcugaa aauggcagag caagauaucc cagacgguuu cauagagagg     2700

gaucuucgua auacccagua uaucgccaag aaggcucugu ccaugcuaaa cgaaauuuca     2760

caccgcgucg uggccacuuc ugggucugua acagacaaac uccgggagga uuggcagcug     2820

aucgacguga ugaaggaauu gaauugggag aaauacaagg cacugggcuu aguugaauau     2880

uuugaggaua gagacggaag gcaaauuggc cgaaucaaag auuggacgaa gcggaacgac     2940

caucgccacc augcgaugga ugcucucacc gucgccuuca cuaaggacgu guuuauacag     3000

uacuucaaua acaaaaaugc aagccuggau cccaacgcca acgagcacgc uauuaagaau     3060

aaauauuuuc agaacgggag agccaucgca ccuaugccac uuagggaguu ccgggcugaa     3120

gccaagaaac aucuggaaaa uacauugauc uccauuaagg cgaagaacaa agugaucacc     3180

ggaaauauua acaagacacg aaagaaaggu ggcgucaaua aaaacaugca gcaaacuccc     3240

agagggcagc uccaccugga gaccauauac ggaucaggca agcaguaccu aacgaaagaa     3300

gagaagguua augccagcuu ugacaugcgc aagaucggua ccgugucuaa auccgcuuau     3360

agggacgcac ugcuuaagcg ucucuacgag aacgauaaug acccgaaaaa ggccuucgcu     3420

gggaaaaaca gucuggauaa gcagccuauu ugguuggaca aggaacaaau gcggaaagug     3480

cccgagaagg uaaaaaucgu cacacuggaa gccauuuaua cuaucagaaa ggagauaagc     3540

ccagauuuaa aaguggacaa gguuaucgau gugggcguca ggaagauucu caucgaccgc     3600

cugaaugagu acggaaacga ugcuaaaaag gcguuuucua aucuugacaa aaacccuauu     3660

uggcugaaua aggaaaaagg caucuccaua aagcgaguga ccauuucagg gaucaguaac     3720

gcccagagcu ugcacguuaa gaaagauaag gacggaaaac ccauccucga cgagaacggc     3780

agaaauauuc caguggauuu cguaaacaca gguaauaacc aucacgucgc uguguauuac     3840

cggcccguga uagacaagag ggggcagcug gucguugaug aagccggaaa uccuaaauau     3900

gagcuagaag aggugguagu gucguucuuu gaggcaguca cucgcgcuaa ccugggccuu     3960

ccaaucauug acaaggauua caaaaccacg gaagguuggc aguuccucuu uuccaugaag     4020

caaaaugagu acuucguguu uccgaacgaa aagacaggcu ucaaucccaa agagaucgac     4080

cuguuggaug uugaaaacua uggguuaauu ucuccuaauc uguuucgugu gcagaaguuc     4140

agccucaaaa acuacgucuu uagacaucac cuggagacca cuaucaagga cacaucaagu     4200

auacuucggg gaaucaccug gauugauuuc agguccucua aaggccugga cacuaucgug     4260

aagguucgcg ugaaucacau ugggcagauc gucagcguag gagaguauug a              4311


<210>  3365
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3365
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gaggcugaaa acaaagauga gagguccucu auagucaagu uggguguucg ggugaauccc      120

uuaacaguag acgaacugac uaacuucgag aaaggcaagu caaucacgac caaugcagau      180

cgcacacuaa aacgagggau gcguagaaac cuccagaggu acaagcugcg gcgcgaaacu      240

cuuaccgagg ugcugaagga gcacaaauug auuacagaag acaccauccu cagugagaau      300

ggaaacagaa cuacauuuga aaccuauagg cugcgggcca aggcggucac ggaggagauu      360

agccuggaag aguucgcucg cgugcucuua augaucaaua aaaagcgagg cuacaaaucc      420

ucuagaaagg ccaagggugu ugaagagggg acucugauag auggaaugga caucgcaagg      480

gaacuuuaua acaauaacuu gaccccuggc gagcugugcc uacaacuccu ggaugccggc      540

aaaaaguuuc uuccagacuu cuaccggucg gaucugcaga augaguugga ccguauuugg      600

gaaaaacaga aggaguauua cccggaaauc cucacagaug ugcugaaaga ggaauuacgc      660

gggaagaaaa gagacgcugu cugggcaauu ugugccaagu auuuugugug gaaggagacu      720

uacaccgagu ggaacaaaga aaagggaaaa acagagcagc aagaaaggga gcauaagcug      780

gaagguaucu acucaaaacg gaagcgagau gaggcuaagc gcgagaaucu ccaguggaga      840

guaaacggcc uuaaagaaaa gcugagcuug gagcagcuag uuauaguguu ccaggaaaug      900

aacacccaaa uuaauaacag uuccggguau cugggagcca ucucugacag gagcaaagag      960

cucuacuuua auaagcagac ggucggccag uaucagaugg agaugcugga caaaaacccc     1020

aaugcguccc uucggaacau gguguucuac cgccaagauu aucuggacga auuuaauaug     1080

uugugggaga agcaggcagu guaccacaag gaacucacug aggaacugaa aaaggagauu     1140

agagauauca ucauuuucua ucagaggcgu uuaaaaucac aaaagggucu gauaggguuu     1200

ugcgaguucg aaagucggca gaucgagguc gacauugaug gcaaaaagaa gaucaaaaca     1260

guuggaaacc gagugauaag cagaucuucc ccucuauuuc aggaauuuaa gauuuggcag     1320

auccucaaua acaucgaggu aaccgugguc ggcaaaaaga ggaaacgccg gaagcugaaa     1380

gaaaauuacu cagccauuuu cgaggagcuu aacgacgcug aacaauugga gcugaauggg     1440

agcagaaggc ucugucagga agagaaggaa cugcuugccc aggagcuguu ugugcgcgau     1500

aagaugacua aaagugaggu uuugaagcuc cuguucgaca acccacagga auuagaucug     1560

aauuuuaaaa caaucgacgg aaacaagacc gguuacgcac uauuccaagc auauucuaaa     1620

augauugaga uguccggcca ugaacccgug gauuuuaaga agccugucga gaaaguggua     1680

gaguacauaa aggccguuuu cgaccuccug aacuggaaua cggacauccu uggguuuaac     1740

agcaaugaag aguuggauaa ccagucguau uacaaacugu ggcaccuccu guauucuuuc     1800

gaaggagaca auaccccaac aggcaacggu cgauuaauuc agaagaugac ugagcuguac     1860

ggguuugaaa aagaguaugc gaccauccuu gccaaugugu ccuuccagga ugacuacggc     1920

ucauugagug caaaggcuau ccauaagauu cucccccacc ugaaagaggg aaaccgguac     1980

gaugucgccu gcguguaugc uggcuaccgu cacagcgaau ccucucuaac aagagaggaa     2040

auagccaaua agguccugaa agacaggcuc augcugcuuc cuaagaacag cuugcauaau     2100

ccagugguug agaaaauccu gaaccaaaug gugaauguaa uuaacgucau cauugauauc     2160

uaugggaagc cggacgaaau acgcguggag uuagcacggg agcucaagaa aaaugccaag     2220

gaaagagagg aacugacuaa aucaaucgcu cagaccacaa aggcgaacga ggaauacaaa     2280

acccuucugc agacugaguu uggauugacg aacguuagua ggacagauau ucuccgauau     2340

aagcuguaca aagagcuaga auccuguggu uauaagaccc uguacagcaa uacuuacauc     2400

ucucgcgaga aacucuucuc aaaagaauuu gacauugagc acaucauacc ccaggcacgg     2460

cuguucgaug acaguuucag caacaagacc cuugaggcca gauccgugaa uauugaaaaa     2520

ggcaacaaga cagcuuauga uuuugucaaa gagaaguucg gggaaucugg agccgacaau     2580

ucguuggagc auuaccugaa caauaucgaa gacuuauuua agagcggcaa aauuuccaag     2640

accaaauaua acaagcucaa aauggcagag caagauaucc cugacgguuu cauagagagg     2700

gaucugcgua auacucagua caucgccaag aaggcucuuu caaugcugaa cgaaauuagu     2760

caccgcgugg uagccacauc ugggagcgug acggacaaac uacgggagga uuggcagcuc     2820

aucgacguua ugaaggaacu gaauugggag aaauauaagg cauugggccu ggucgaauac     2880

uuugaggaua gagacggaag gcaaauuggc cgaaucaaag auuggaccaa gcggaacgac     2940

caccgccauc acgcgaugga ugcuuuaacu guggccuuca caaaggacgu guuuauacag     3000

uauuucaaua acaaaaaugc aucccucgau ccaaacgcca acgagcaugc uauuaagaau     3060

aaauacuuuc agaacgggag agccaucgca cccaugccuc ugagggaguu ccgggcugaa     3120

gccaagaaac accuugaaaa uacccugauc ucaauuaagg cgaagaacaa agucaucaca     3180

ggaaauauua acaagacucg aaagaagggu ggcguuaaua aaaacaugca gcaaacccca     3240

agagggcagu ugcaucucga gacgauauac ggaagcggca agcaguaucu gaccaaagaa     3300

gagaagguga augccucuuu ugacaugcgc aagaucgaga caguguccaa aagugcuuac     3360

agggacgcac uacugaagcg ucuuuaugaa aacgauaaug accccaaaaa ggccuucgcu     3420

gguaaaaaca gccucgauaa gcagccgauu uggcuggaca aggagcaaau gcggaaagua     3480

ccugaaaagg ucaaaaucgu gacuuuggag gccauuuaca ccaucagaaa ggagauaucu     3540

cccgaucuga aaguugacaa ggugaucgau gucgggguga ggaagauuuu aaucgaccgc     3600

cucaaugaau auggcaacga ugcuaaaaag gcguuuucca aucuggacaa aaacccaauu     3660

uggcuuaaua aggagaaagg aaucucaaua aagcgaguua caauuagugg caucagcaac     3720

gcccagucgc ugcacgugaa gaaagauaag gacgggaaac cuaucuugga cgaaaacgga     3780

agaaauauuc ccguagauuu cgucaacacu ggcaauaacc accauguggc uguguacuau     3840

cggccaguca uagacaagag gggucagcuc guuguggaug aggccgggaa ucccaaauac     3900

gaacuggagg agguaguggu cuccuucuuu gaagcaguga cccgcgcuaa ccuaggacug     3960

ccuaucauug acaaggauua caaaacgaca gagggcuggc aguuccuuuu cucuaugaag     4020

caaaaugaau auuucguuuu uccaaacgag aagaucgguu ucaauccgaa agaaauugac     4080

cuccuggaug uggagaacua cggcuugauc agccccaauu uauuucgugu ccagaaguuc     4140

ucacugaaaa acuauguguu uagacaccau cucgagacca cuauaaagga cacaaguucc     4200

auccugcggg ggauuaccug gaucgauuuc aggucuagca aaggacuuga cacuauuguu     4260

aaggugcgcg ucaaucacau cggccagaua guauccgugg gggaauacug a              4311


<210>  3366
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3366
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugguggau       60

gaggcugaaa auaaagacga gagguccucu auagucaagu uggguguucg ggugaacccc      120

uuaacaguag augaacugac uaauuucgag aaaggcaagu caaucacgac caacgcagac      180

cgcacacuaa aacgagggau gcguagaaau cuccagaggu acaagcugcg gcgcgaaacu      240

cuuauggagg ugcugaagga gcacaaauug auuaccgaag auacaauccu cagugagaac      300

ggaaauagaa ccacuuuuga aacauauagg cugcgggcca aggcggcuac cgagaaaauu      360

agccuggagg aguucgcccg cguccucuua augaucaaca agaaacgagg cuacaagucc      420

ugcagaaagg caaaaggugc cgaagaaggg acgcugauag acggaaugga uaucgcuagg      480

gagcuuuaua auaacaauuu gacuccuggc gaacuguguc uacaacuccu ggacgcaggc      540

aagaaauacc uuccagauuu uuaucggucu gaccugcaga acgaguugga ucguauuugg      600

gagaagcaga aagaauacua uccggagauc cucaccgaag ugcugaagga ggaauuacgc      660

gggaaaaaga gagacgccgu uugggcuauu ugcgccaagu cguucgugug ggaggaguac      720

uacacagaau ggaauaaaga gaagggaaaa acugaacagc aagagaggga acauaagcug      780

gaggguaucu auucaaaacg gaagcgagau gaggcgaagc gcgaaaaccu ccaguggaga      840

gucaauggcc uuaaagagaa gcugagcuug gaacagcuag ugauaguacu gcaggagaug      900

aacauucaaa ucaacaauag uuccggguac cucggagcaa uuucugacag gagcaaagag      960

cuguauuuua acaagcagac cguuggccag uaccagaugg aaaugcuuga caaaaauccc     1020

aacgccuccc ugcggaauau gguguucuau cgccaagauu acuuggacga guuuaacaca     1080

cucugggaaa agcaggcuga guaucacaag gaacugaccg gugagcucaa aaaggagauc     1140

agagauaucg ucauuuucua ccagaggcgu cugaaaucac aaaaggggcu agugggauuu     1200

ugugaguucg aaagucggca gauagaagug gagaucgacg gcaaaaagaa gauuaaaacg     1260

gucggcaauc gaguuauccc uagaagcucu ccacucuuuc aggaguucaa gauauggcag     1320

auucugaaca auaucgaagu gacuguagug gggaaaaagg gaaaaaggcg caagcaaaaa     1380

gauaacuccc ccacacuuuu ugacgguuug gaugacaccg agcagcugga acucaauggc     1440

ucacgguacc uguaucagga ggaaaaggag cuucuggccc aggaauuguu cgucagagau     1500

aagaugacua aaagcgacgu gcucaagcug uuauucgaua acccucaaga gcuggaccua     1560

aauuuuaaaa caaucgacgg gaacaagacc ggauacacgu uauuccaggc auauaguaaa     1620

augauugaga ugucuggcca ugaaccaguu gauuuuaaga agaccgugga gaaagucgug     1680

gaacacguaa aggcuguuuu cgaccugcuu aauuggaaca cagauaucuu ggguuuuaac     1740

uccaaugagg agcuggacaa ccagagcuac uauaaacucu ggcaucuguu auacucguuc     1800

gaaggggaua auacucccac cggcaacgcc aggcuggugc agaagauuac agagcuuuau     1860

ggauuugaaa aagaguacgc gauaaucuug gccaauguca cuuuccaaga cgauuacggc     1920

ucucucuccg caaaggcuau ucacaagauc cugccucacc uaaaagaagg gaaccgcuau     1980

gacguggccu gcgcuuacgc cggauauaag cauucagaga guagccugac caaagaggaa     2040

aucgcaaaua aggcccucaa agaucgacug acacuuuugc caaagaacaa ucugcggaac     2100

ccggucgugg agaagauuuu aaaucagaug guuaacguga uaaaugcuau cauugacacc     2160

uacgguaaac ccgaugaaau ccguguagag cucgcgagag aacugaagaa aaacgcaaag     2220

gagagggagg aacuuacuaa auuuauugcc gagacgacac gcaagcacga agaguucaaa     2280

auccuguugc aguccgaauu uggccucacc aaugucucuc ggacugacau acugagauau     2340

aagcuauaca aggagcugga gagcuguggg uauaaaaccc ucuacucaaa cacauacauc     2400

ccuagggaaa agcuguucag uaaagaguuu gauauugaac auaucauucc acaggcucga     2460

cuuuucgacg acuccuucag caacaagacc uuggaggccc gcucuaucaa ucuggagaaa     2520

ggaaacaaga cugcauauga uuuugugaag gaaaaauucg gcgagucagg ugccgacaau     2580

aguuuagauc acuaccucaa caauauagaa gaccuguuua agagcgggaa aauuuccaag     2640

acaaaauaua acaaacuuaa gauggcugag caagauaucc ccgacggcuu cauuaaucgg     2700

gaucugagaa acacgcagua caucgccaaa aaggcacuau cuaugcucaa ugaaauaucg     2760

aggcguguug uggcgaccag cggaacuauc acagacaaac ugcgccagga uuggcaauug     2820

gucgacguga ugaaggagcu gaacugggag aaauauaagg cuuuaggccu cguagaauac     2880

uuugaggaua aggacgggcg gcagauugga agaaucaaag auuggaccaa gaggaaugac     2940

caccgacauc acgccaugga cgcacugaca guggccuuca cuaaagauau uuuuauccag     3000

uauuucaaca auaagaacgc uucccuuaau ucaaacacca acgaauacgc cauaaagaau     3060

aaguacuuuc agaacggucg ggcaauugcu ccuaucccac ugcgcgaguu cagaguugaa     3120

gccaagaaac auuuggagaa uacgcucauc aguauuaagg cgaaaaacaa ggucgugacc     3180

ggcaauauca acaaaacaaa gaaaagggga ggggugaaua aguaucugca acagacuccc     3240

cggggccagc uacaccugga gaccauuuac gguucuggga agcaguaucu uacaaaagaa     3300

gagaagguca acgccagcuu ugacaugaaa aagauagcua cuguuuccaa aucagcauac     3360

cgagaugugc uccugaagag auugcaugaa aaugacaacg auccgaagaa agccuucacc     3420

ggcaagaaua gccuggacaa acaaccuauc ugguuagaua aggagcagac gcgcaaagug     3480

cccgaaaagg uaaagacagu cacccucgag acuauuuaua cagugaggaa agagaucucu     3540

ccagaccuga agguugauaa agugauugac gcuggagucc guaagauccu uaccggccgg     3600

cugaacgaau acgggaauga ugccaaaaag gcauuuucca acuuggacga gaauccuaua     3660

uggcucaaca aggaaaaagg aaucaguauu aagagaguga cuaucagcgg cauuucuaau     3720

gcgcaguccc ugcacguuaa aaaggacaaa gaugguaagc ccauccuaga cgaggauuca     3780

aggaacauac caguggacuu cguaaacacc gggaauaacc accaugucgc cguguaucgc     3840

cgacccguga uugauaaaag aggacagcug gucaucgacg aggcuggcaa uccuaaguac     3900

gaacuugagg aaguuguggu aaguuucuuu gaggccguga cacgggcaaa ccucggucaa     3960

ccaaucauug auaaggacua caaaacgagc gaaggcuggc aguuccuguu uucgaugaag     4020

cagaaugagu auuucgucuu uccgaacgag aaaaccgggu ucaaucccaa ggaaauagau     4080

uuguuagacg cugagaacua cggagugauc uccccuaauc uguuuagggu ucaaaaauuc     4140

gcccacaaga acuauguguu ucgccaucac cucgaaacua caauuaagga uaccucuagc     4200

auccugaaag gcauuacaug gaucgacuuc cguucaagua aggggcuuga ugcaauaguc     4260

aaagugagag uuaaucacau cggacagauu guguccgucg gcgaguacug a              4311


<210>  3367
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3367
augaaguaca ucaaauauau ucugggccuc gacauaggga gcaacuccau cggaugggcc       60

cuuauucagc uggauuuguu acaaaagacc aaaagaauca uugcugcagg uucuaggauc      120

auacccaugg accagaaggu gcugucagau uucggcaaag ggaucacaau uaguccuacu      180

gcccagcgga cgagcuaccg caaugcgcga cacugccgug agagacaucu acucaggcgg      240

gaacgccugc acagaguccu ucauauccug ggauuuuugc caacccacua ugacaagcag      300

auugauuucu ccaagaggua cggcaaauuu guugacaaca cagagccgaa gaucgcuuau      360

aaugaaggua aauuccucuu uauggauucu uuccaagaga ugcugucgga cuuuaaguca      420

caucagcccg aucuguucua cacuaaaaag aacggggaag agagcaagau accuuaugac      480

uggaccauuu acuaccuccg gaaaaaggcc uuaagucaga aaaucuccaa ggaggaacug      540

gcauggauua uccuuaauuu uaaccagaaa cgcggauauu accaauugcg agaggaagag      600

ggcaauaagg agaacgauuu cgaaaucauu ucuagcaaaa uaaucuccgu gauuaagaag      660

gacaaagaua agaaauauaa uaaguacugg uaugacauca uacuggagaa cggccuagua      720

uaccaggccg cuuuuuauaa ugacuucgug uacaacugga uuaaucagga uagagaauuu      780

aucaucgaga ggacaucacu caaaaacggg gcagaaaagg ucaagcugag uuaccuucca      840

accuucgacg agauugauaa aauggacaag aaagagcagg auaaguauua caaaaagauc      900

aagauuaaaa cugaaaauag cauaggaaac aagacagugg guuguuauau cuacgaccac      960

auucugcaaa accccaauga gaaaaucaag ggcaaauugg uucggaccau cgaacguaag     1020

uauuacaagg aggaacucaa agccauucug gagaagcaga uagcuuuaca gccugagcug     1080

uucacggauc aacuccuugc cgacugcauc cgcgaacugu auucuaaaaa cgaggcgcag     1140

cagagaaauu uggcagccag ggauuuugug caccuacugg ucgaagacau uaucuucuac     1200

cagcggccac uccgacgcca aaaguccacu cuggcuaacu guucacuuga gagcagaagu     1260

uacauugaua aagacucuca uaccaggaag gaagcacccc ugaaagugug cccuaagucc     1320

aauccauauu accaggaguu ucggguauug caguggcucc agaaccugaa gaucuaugag     1380

auagacagcg aucaagaagu uacacaccag uucaucaaaa cuuuagagga caagcagcag     1440

cuguuugaau uucuaauggc ccaaaaagag auugauugug aggaacuccu gaaguacuuu     1500

cuuaaucaga ccuauccgaa cgcuaaagag aaggccuuga agucggaacu gaaaaagugg     1560

aaagacacau accgcuggaa uuauguguac gauaucgggg agaagucuuc caaaaaguac     1620

cccaugaacg aaaccagaua ugagcucaag aggcaucugg agaaagucgc gaaucuuccu     1680

gacgauuucc ugucaaguga aguggaguac uugcucuggc accugauuua uagcgugaag     1740

gacaaagucg ccuacgaaaa gggauuaaag aaguuugcac agaagcacca ucuggauaaa     1800

gacuccuucg uugagucuuu uaagaaauuc aacccauauc ccagcgaaua cggcucauuu     1860

agugagaagg cuauccguaa acuacucccu cugaugcggu ucggugccca cugggauuuc     1920

aaucauauag acaagaacac gcagaaacga auugaggauc uuaucacugg gguggaaaau     1980

gaggaaauua gaacaaucuu gagggagaag gcugaaaagu aucaacugga gaaagagacc     2040

gacuuucagg accucccacu gugguuagcc caguacauag uauacaaccg ccacgcagaa     2100

gccuccagcc uggagaagug gacuucugug aacgaucuug aaacauauuu gaaugaguuc     2160

aaacaacauu cacuccggaa ccccaucguc gaacaggugg uuauggaaac ccugagagug     2220

gucagggaca uuuggcagca guacggacaa ggccaggcua guuucuucaa ugagauccac     2280

auugaacuag gccgcgagcu gaagaaaacg gcgaaggaac gagagaagcu cagcaaacag     2340

aaccagaaga augaggauau caaccugucc auaaaagaaa aacuuaaaga guuggaaaag     2400

uauaauaccc gggcauacuc uauuacacac caagagaagu auaaacugug guuagaacag     2460

aagcauaucu cgccguacac ugggcagauc auuagcccug agaaccucuu uaccgacaau     2520

uaugagaucg aacacauuau accccagucc cguuucuuug augacucacu gaguaacaaa     2580

gugguaugcg agucuaucgu uaauaaggcc ccauacaaag auagacaacu uggacuggag     2640

uucauuaaga accagggugg caggaucgug aaggaguuga gcaaagaaaa uaagacaauu     2700

aaaaucuuua cugaggagca guaccgcacc uucauaaagg aacauuauuc caacaauccu     2760

gagaaaacaa agaagcuccu gcuagaagag auccccgaga aaauggucgc ucggcaaaug     2820

aacgacaccu guuacauuuc aaaguuuauc agcgaaauuc ugucuaaaau cgugagaucc     2880

gaugagcagg acgaaggggu ccacaguaag aacgugauac ucugcacugg aaaaauuacg     2940

agcauccuga ggcaggauug gggccuuaau gacaucuggg auaacuugau ucugccacga     3000

uucgagcgca ugaauaacuu acuccagaag gaaaaauuua caauauauuc uaucgagaag     3060

caaaagaaaa uucccguugu gccuaucaau gaccaguuca aguccuucca gaaaaagcgg     3120

auugaccaca gacaucacgc cauggaugca aucauaaucg ccuguaccac uagggagcau     3180

auuaacuaca ucaauaacca gcacgcucug gaaaaagaga aggacaagaa agaaaagcaa     3240

gaggaacgug auaaacuucg cgagaagcug ugcauuaaaa aguauaauaa ccggucagag     3300

gguaauuaca guuggaucuu uaagaaacca ugggacaccu ucacacagga aguaagaguc     3360

gcccuagagg auauaguggu uagcuuuaag cagaaccuca gggugauuaa uaaagcaacc     3420

aacuauuacg aacgaugggu cgaggaaaau gggaaguaca ucaaaaagau ggugaagcag     3480

gagggcacua acugggcgau ccggaaaccg cugcaugagg aaacaauuuc gggaaagauc     3540

uauuuggacc gcgagaaaau aacgaagaac gauauucuga ccgcuacuag aaaaacagua     3600

gacuccucuu ucgaugacaa gaggaucaaa aagauuaccg auacaggcau ccaaaagaua     3660

uuacucaauu accugaaaua uaaggggagc cccgaaauug ccuuuucacc ugagggacuu     3720

gaggaacuga acaaaaauuu gaguaucuac aacgacggua agccacacaa acccaucuau     3780

aaggugcgga uuuacgagaa gggcuccggg cgauucgcac ucggagaaaa aggcagaaag     3840

uauaaaaagu acguucaggg ugccucuaau cuguacuuug ggaucuauca gggcgcuaaa     3900

cgcagcuucg ccacuauucc ucuaaacgag gucauagaaa ggcagaagca aggacugcca     3960

uccgugcccg aguacaauga gaagggcgac cuucuccugu uuucauugag cccgaacgau     4020

cugguguaug ucccuaucga aggggagauu aucgaaaaua ucgacuuccg uaacuuaagu     4080

aaagagcaga aggaacggau uuacaauguu aacgauuuuu cuuccaccug uuauuucacg     4140

ccaaauagaa uagcaaaagc uaucuucccc aaggaggugg accuccagag gaaaggagau     4200

aagcugagcg guucguacga caccaagaca gccucacuug agggcauuca aaucaaagaa     4260

gugugcauua agcugaaagu agaucgcuug gggaacaucu cuaaggcgug a              4311


<210>  3368
<211>  4314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3368
augaaguaca ucaaauauau ucugggccuc gacauaggga gcaacuccau cggaugggcc       60

cuuauucagc uggauuuguu acaaaagacc aaaagaauca uugcuacagg uucuaggauc      120

auacccaugg accagaaggu gcugucagau uucggcaaag ggaucacuau uaguccuacg      180

gcacagcgga ccagcuaccg caaugcccga cacugccgug agagacaucu acucaggcgg      240

gaacgccugc acagaguccu ucauauccug ggauuuuugc caacacacua ugacaagcag      300

auugauuucu ccaagaggua cggcaaauuu guugacaaca cugagccgaa gaucgcguau      360

aaugaacgga aauuccucuu uauggauucu uuccaagaga ugcuggcuga cuuuaagaua      420

caucagcccg aucuguucua caccaaaaag aacggugaag agucgaagau uccuuaugac      480

uggacaaucu acuaccuccg caaaaaggcc uuaucacgaa aaauuagcaa ggaggaacug      540

gcauggauca uccuuaauuu uaaccagaaa agaggguauu accaguugag ggaggaagag      600

gaggaaaaua aggagaacga uuucgaaauu auaaguucca aaaucauuuc ugugaucaag      660

aaggacaaag auaagaaaua uaauaaguac ugguaugaca uaauucugga gaacgggcua      720

guauaccaag ccgcuuuuua uaaugacuuc guguacaacu ggaucaauca ggaucgggaa      780

uuuaucauug agcguaucag ccucaaaaac ggcaccgaga aggucaagcu guccuaccuu      840

ccaacuuucg acgaaauuga uaaaauggac aagaaagagc aggauaagua uuacaaaaag      900

auaaagauca aaacagaaaa uucaauuggc aacaagaccg ugggguguua uaucuacgac      960

cacauccugc agaaccccaa ugagaaaauu aagggaaaau ugguucgcac gauagaaaga     1020

aaguauuaca aggaggagcu caaagcaauc cuggaaaagc aaguggccuu acagccugag     1080

cuguucacug aucagcucuu ugcugacugc auuagggaac uuuauaguaa aaacgaggcc     1140

caacagcgga aucuggcggc acgagauuuc guccacuugu uuguggaaga caucauuuuc     1200

uaccagcgca gccuaagaag gcagaagucu acccuggcca acuguucccu cgagucacgg     1260

agcuacaucg auaaagacag ucauacacgc aaggaggcuc cacugaaagu augccccaag     1320

ucuaauccuu auuaccaaga auuuagaguu cuucaguggc ugcagaacuu gaagauauau     1380

gagaucgaca cugaucagga agugacccac gaguucauua aaacacucga ggacaagcaa     1440

caguuuuucg auuuucugau gaaucagaaa gaaaucgacu gugaggaauu acugaaguac     1500

uuccuaaacc agaccuaucc aaaugccagg gagaaagcac ucaaguccga acugaagaaa     1560

uggaaggaua cguaccguug gaacuauguc uacgacauug gugagaaaag cucgaagaaa     1620

uacccgauga augagacucg guaugaacuu aagcgacauu uggagaaggu ggcuaaccug     1680

cccgaugacu uucucucuuc cgaaguggag uaccugcuuu ggcaccugau cuauucaguc     1740

aaagauaagg uugccuacga aaaaggcuug aagacauucg cgcaaaaaca ccaucucgac     1800

aaggauaguu uuguggagag cuucaagaaa uucaagccuu auccauccga guacgggucu     1860

uuuagcgaaa aagccauaag aaagcuguua ccccugauga gguucggauc auauugggac     1920

uuuaaucaca uugauaaaaa cacccagaag cgcaucgagg accuaauuac uggcguagaa     1980

aaugaggaaa uccggacaau acucagagag aaagcagaga aguaccagcu ggaaaaggag     2040

accgacuucc aggaucuucc uuuguggcug gcucaauaca ucguguauaa caggcaugcc     2100

gaagcuaguu cccucgagaa auggacgagc gucaacgacc uggagaccua cuuaaaugaa     2160

uuuaagcagu auucucugcg caacccaauu guggagcagg uugugaugga aacacuucga     2220

gucgugcggg auaucuggca acaguacggu caggggcagg ccucauucuu ugacgagauu     2280

cacaucgaau ugggacguga gcucaaaaag acugcaaaag agagagaaaa gcugaguaag     2340

caaaaucaga aaaacgagga uauaaaucua agcauuaagg aaaaacugaa ggagcucgaa     2400

aaauauaaca ccagggccua cuccaucaca caucaggaga aguauaagcu guggcuugag     2460

cagaaacaca ucucucccua cacuggccaa auuaucucgc cggaaaauuu guucaccgac     2520

aacuacgaga uugaacacau aaucccucag agccgcuuuu ucgaugacuc ccugucaaau     2580

aagguaguuu gcgagaguau ugugaacaaa gcucccuaua aggaucggca guuaggccuc     2640

gaauuuauca aaaaucaagg gggaagaauu gucaaggagc ugucuacaga gaacaagacc     2700

aucaaaauau ucacugaaga acaguacagg acguuuauca aggaacauua uagcaauaac     2760

ccagagaaaa caaagaaacu ucuguuggag gaaauuccug agaagauggu ggcgcgacag     2820

augaaugaca ccuguuacau cuccaaguuc auuucagaaa uccucagcaa aauaguccgc     2880

ucugaugagc aggacgaagg ugugcacucc aagaacguua uucugugcac uggcaaaauc     2940

accagugcac uaaagcaaga uuggggagug aacgacguau ggaaugaccu gauccucccc     3000

cgguucgaga gaaugaacga ucugacacag agcacccuuu uuacuacaua uaaugagaaa     3060

uaccagaagu auuugccaac gguccccaug gaauacucua acggauucca gaaaaagagg     3120

auugaccauc gucaccacgc cauggaugcu auaaucauug ccugugcaac ccgcgagcau     3180

aucaauuaca uuaacaauca acacgcccug gaaaagggca aagacaagaa agagaagcag     3240

aaagaacaug auaaguuacg ggagaagcuc ugcaucaaaa aggacaacaa agguuccgag     3300

gaaaauuauu cauggauauu uaagaaaccu ugggauacuu ucacacagga ggugcaggcu     3360

gcccuggaag ggaucguugu gaguuuuaag caaaaccuua gagucauuaa uaaggcaacc     3420

aacuacuaug agaggugggu ggaagagaau ggcaaauaca ucaagaaaau gguaaagcag     3480

gagggaacaa acugggcgau ucgaaaacca cugcacgaag agacuaucag cggcaagaua     3540

uaucuagacc gggaagagau uaccaagaau gauauccuca cggcuacccg caaaacagug     3600

gacucguccu ucgaugagaa gagaaucaaa aagauuacug acaccgggau ccagaaaaua     3660

cuguugaacu accugaagua uaaaggaucu ccggaaauug ccuuuagccc cgaggguuua     3720

gaggagcuca acaagaaucu gucaaucuac aacgacggca agccucauaa accaauuuac     3780

aagguuagga ucuaugaaaa agggagugga cgguucgcac uuggcgagaa gggucgaaaa     3840

uacaagaagu auguccaggg ggcccccaau cuguacuuug gcauauauca aggagcuaac     3900

aaaagauccu ucgccacaau uccuuugaau gaggugaucg aacgccagaa gcagggcagg     3960

ccaucuaucc ccgaguacaa cgaaaaaggg gauccgcucc uguuuagccu auccccuaau     4020

gaccuggugu augucccagu ugagggagaa auuaucgagg auauugacuu ccagaaccuu     4080

ucaaaggagc aaaaagaacg uauauacaau gugaacgauu ucagcaguac uuguuacuuu     4140

acccccaauc ggaucgcaaa ggcuauuuuc ccuaaggagg uggaccucca gagaaaaggu     4200

gauaagcugc ccggcucuua ugacacgaaa acagccuccu uggaagggau ccagaucaag     4260

gagguaugca uuaaacugaa gauagauagg uuaggaaaca ucagcaaggc guga           4314


<210>  3369
<211>  4317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3369
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc ccugauaaaa       60

cagaauuucg agaacaagua cggugaaauc uugggcaugg gguccagaau uauccccaug      120

ucucaagaua uuuuaggaga guuuggcaaa gguaauucag ugagucagac ugcugcaagg      180

acggacuauc gggccacccg ccgacugcgu gaaagauucc uacucaggcg ggagcgccug      240

cacagagucc uuaacguucu gaauuucuug ccugaacauu acgcgagcca gaucgauuuc      300

gacaagaggu uugggaaauu caagguggag acagagccaa agcucgcuug gaaaaacaua      360

gauggaaagu uuuccuuccu guuucagacu ucuuucaaug aaaugcugga ggacuuuaaa      420

gcccacggcc aagaucucaa gguaccguau gacuggacca ucuacuauuu acggaaaaag      480

gcacugucgc agaagauuga aaaagaggag cuugugugga ucuugcugaa cuucaaucag      540

aagcgcggcu acuaucagcu acgaggggaa gaggaagagg aaaaccccaa uaaacucauu      600

gaguuuuacu cacugaagau cauagauguc cuugccgacg agccucaaaa aggaaagagc      660

gauauuuggu acagucuggu guuggaaaac gguuggaucu auagaagguc cucuaaaaca      720

agccuccugg acuggaagga uaagauucgg gacuucaucg uuaccacuga cuuaaaugau      780

gacggcuccg ugaaaacaga uaaggagggg aacgaaaaac guucauuccg cgcuccagga      840

gagaaugacu ggacccuggu caagacgaaa acugaacagg agaucgauag aaguaggaag      900

accgugggca acuacauuua ugagacacuc cuucagaacc ccaagcagaa aauaaagggu      960

aaacugguac ggacuaucga acgaaaguuu uacaaagagg aauugaagcg cauucuagag     1020

aagcaaaaag aauuucauag agagcuggag agcaaugacc ucuauaacga uugcaucagg     1080

gaacuguacc ggaauaacga ggcacaccag cuuacccugu cuaagaaaga cuuuguucau     1140

uuguucaugg aagauauaau uuucuaucag cgcccucuca agucccaaaa aucaagcauc     1200

aguaauugua cacuggaguu cagaaaguac aaggacgaga acgggaucga acacacccag     1260

uuuuuaaaag ccauuccaaa gucuaauccc uacuaucagg aguucaggau cuggcagugg     1320

auuuuuaacc ugaauauaua caaaaaggau aacgacgaca augugacgaa agaauuucua     1380

aacacuacac gugauuuuga gaaucucuuc gaauuucuga acaaucggaa ggagaucgag     1440

caaaaaccuc uuuugaaguu ccugcucgaa cagaaggagu ucaaaggaaa gcugcuuaac     1500

gcugaaaccg agaaauuucg auggaacuau gucgaagaca agaaauaccc augcaaugag     1560

acuagaacaa ugauuuccga gaggcugaag aagguggaaa acaucagcga ugacuucuug     1620

acccgcgaga uggaacagaa aaucuggcac auuauauauu cggugaauga uaagacggag     1680

uacgaaaaag cccucaaguc uuuugcggag aaaaacaauc uggacgagaa cuccuuauuc     1740

gaagcauuua agaagcugcc gcccuucaaa ucagaguaug gcaguuuuag cgaaaaggcc     1800

aucaaaaagc uacucccucu gaugcggcuu ggcaaauacu gguccuacgc uaagauugau     1860

uuguauucua agaauagaau ccagaaaauu aucaccgggg aguucgacga gaacauaaag     1920

gauagggucc gcgaaaaagc cauccgacug acagcagaga augacuuuca aggacuccag     1980

cugugguuag cucaguacau uguuuauggu cggcauagcg aagccucaau gaucggcaag     2040

uggaacagug cggaugaccu ggaggaguuc cuuaaagaau uuaagcagca cuccuugcgu     2100

aauccaauug uggagcaagu aaucacugaa acccucagaa uagugaaaga uauuuggcug     2160

aaguacggga acggagccaa ggacuucuuu aaugagaucc auauugaacu aggcagggag     2220

augaaacaga caaaggacga acgcgcaaac gcuacuaaaa ccaucacaga gaaugagaac     2280

accaaccugc ggauaaaggc ccuccuggcu gaaaugauga augaucacag cgucgagaac     2340

gugagacccu auucuccuau gcagcaggaa auccuuaaaa uuuacgagga cgguaucuug     2400

aagucagaua uugaaaucga cgaugacaua cugaagauua guaaaacugc ccaaccaagc     2460

uccucugauu uaaagaggua uaaacucugg cuggagcaga aguacaaauc gcccuacacg     2520

gggcagauca ucccgcuuaa uaagcuguuc acaccugagu augaaauuga gcacaucauu     2580

ccccaaagcc gauacuucga cgauuccuuu ucaaacaaga uaaucuguga aagugcaguu     2640

aauaaauuga aggacaacua uauuggccuc ggauucauca aacaguuugg cgggaccauu     2700

aucgagugcg gauucgguaa gcgcgugaaa gucuuugagg ugggcgaaua cgaggaguuc     2760

guaaagaagc auuaugccaa uaaccggggg aaaagaaaua agcugcuacu ggaagaaaua     2820

ccagagaaaa ugaucgagag gcagaugaac gauacucguc acauuucuaa guacaucagc     2880

ggcauucucu cccauaucgu ucgcguggaa gacggaaccg augagggugu caauagcaaa     2940

aacgugauac cugggaaugg caagauuaca acccagcugc ggcaagacug gggacuuaac     3000

gacgucugga augaauugau ccugcccaga uuugagagga ugaaccaguu aacuaacuca     3060

acagauuuca cgucuuggaa ugaaaaccac cagaaguauc ucccaaccgu gcccguugag     3120

uuuuccaaag gcuucaguaa gaaacgaauc gaccaucggc accacgcucu ggaugcgcuu     3180

gugauugcau gugccacuaa ggaccaugua aaucugcuaa acaaucagag cgcuaaaucu     3240

gccacaaagc gcuacgaucu caaaaagaag cugaugaaau uugagaagac cgucuacaaa     3300

gacccucaaa cagaaaagag aauagagagg gaagugccaa aacacuucuu gaagccgugg     3360

gagacuuuca ccguugaugc aaagaaccgg cuggaaacga ucauuguguc cuuuaaacag     3420

aauuuacgag ucaucaacaa ggccaccaau uauuacgaga aauaugugga gaaggacggg     3480

guaaugauga aagaaagagu ggagcagaca ggaacuaacu gggcuauucg caagcccaug     3540

cauaaggaaa ccguuucagg uaaaaucgau cucccuuggg ucaaggugcc aaaaggcaag     3600

auacugacag ccacuaggaa aagucuugac accagcuucg aucugaaggc aaucgcgucg     3660

auuacggaca cagggaucca gaagauuuug aaaaauuacc ucgaguuuaa ggaguccccc     3720

gaacuggcuu ucucuccuga gggaaucgaa gacaugaaca aaaauauaga gaaguauaac     3780

ggcggucguc cacaccaacc cauuagcaaa gugcgggucu uugaacuagg gucaaaguuc     3840

cagguuggcc agaccggaaa uaaaaaggau aaguacgugg aggccgcaaa aggcacuaac     3900

cuguuuuucg ccaucuauga ggacaagaaa gggaagagaa guuacgaaac aaucccgcuu     3960

aaugagguga uugaaaggca aaaacaggga cucuccguag ucgaucugaa gaacgugaac     4020

gacuuuuucu ugugcccuaa ugaucugguu uacaucccau cuggcgacga guuugaaaac     4080

cgcgguagca uagagauuga gaauaucacc aaggaaaaau ccgagcgauu cuauaaggug     4140

gucucauuua gcgggaguca gauuuucuuc gugagacacg auaucgcugu uucuauagug     4200

aacaaaggag aauuuuccac uuuaaauaag auggagcggg ccauugacgg cagcauggua     4260

aaagaaaucu guaucaagcu caagauugau aggcugggug acguccuuaa agcauga        4317


<210>  3370
<211>  4317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3370
auggugaaga aaggcuacac caucgggcug gacauuggaa caacuagcgu cgguugggcc       60

guuacggaug acgauuucga gcucgugaac aagaaaaugu ccauaaagga caaaucuggc      120

ucaaccauca agagaguaag gaagaaucuu uggggagugc ggcuguuuga ugaagcuaaa      180

acagcagaga acacucgccg aacccguggg gucagaaggc ggauucgcag aaggaaggaa      240

cgguugaauu auuuacaggg caucuucagu agcgagaugg ccaaaaugga cgaaaacuuu      300

uucaugcgcc uggaggaguc cuuuuucaag cuaucugaua aaucggaaca aaauaaguuu      360

cccuacgcgc uuuucaagaa cgagauugaa gagaaggcuu auuaccgaca guuuccuaca      420

aucuaucacc ugagaaagca ucuuauggag cacgaagagu cagaccugag gaaaauauac      480

uugggucucc aucacauccu gaaguaucgg gggcauuucg ugcaggaagg acagaaaaug      540

gauguugcca auauugacau ccaaggcaac cugaaggagc ucuuuaaugc auuaaacgaa      600

aauuucagca acaaagagga ugacuuugag caggaacgua ugaaggaugg cgugaagcgc      660

gccaaagucc uguucgacac cgagaagcuc gcugcagugg aagagauuuu gaaagaaaag      720

aucaguaaau ccuggaaggu aaauaagaua ucuaaaggga uucuggccga gaagaaagac      780

cuaccaaucu ucaaggcuau uguuggaaac aaaaucgauc ucgccgguau cuuugacugc      840

ccggauuacg cggagaaggu gaaugaaagc auuaaggagc ugaaagacaa ggucuacuuc      900

aacaaagaag auuuugagga aacuuucgag aaacuuuccc agcuguugaa agaggaggaa      960

cucgaaauac ugaucuuagc aagaaagugu uaugaggccg ugcugcucgc ugacauuaug     1020

cagggcgagc aaucacuuag ugaugccaug guggcaaagu acaacgacca caaaaaggau     1080

cuggucaucu uuaaaaaguu cguuauagac uauuuuagcg aagaugugua cuucgacuug     1140

uuuaaaaaua cacagcuagc ugggaacuau cacucuuacg uaaagggaga gaggaaaggc     1200

aauaaguuca accaugccuc ccuggacgau uuuuauaagu ucgugaaaaa ggaacucgac     1260

aaaaaggcgg aggaacuguc agagcacccc gucuacaaac agauucuuca gaagaucgag     1320

cuggaaaccu uuuugccuaa gcaacggaug uucaaaaaug gugccauccc auaccaggug     1380

caucuccacg agcugcgagc aauuaucaag aaacaggaaa agcgcuuccc cuuuuuagcu     1440

gagaaaauua uaaucaagga uaaccaaaag gacauuagcg aauauaaaau cgagacgcug     1500

augaaguuca gaaucccuua cuauguuggg ccacuaauug aggccaggga aggagagcuc     1560

ggcaaaaagc ggacuaauua cagucgcuuu gcuuggaugc agaaaucucc cgaaucccag     1620

aacauaggca ucaccccuug gaauuuccac agcguggucg auaaggacgc cucggcaucu     1680

gaguuuauug aaagaaugac aggguucugc uccuauuuuc cagaggagcc ggugcugccc     1740

aagaacucac uuaucuacca gcaauucacu guauuucagg aauugaguau uguuggauau     1800

uacgaugagc agaauaggaa acauuaccug gacggugaaa cccgucggcg aaucuucgag     1860

gaucucuuua agaaaaacag aacagugagc gccggccugc uuaggaagug gcuggucugu     1920

aaucagcgca ccucccaaga ggaaaaagag auacgguugu ucgggcucga ccugaagaaa     1980

aacggauuag gcucugguaa uuuuaacacg aaguauacua cauaccugga ucuaaaggac     2040

gcugggauca ccgaaaaaca gcucaagcag cacaaagauu ucuucgagaa gauugugaaa     2100

uaucagacua ucuuugaaga caagaagauu cugaaaagaa ggaucauacu uuugaacgag     2160

gagcaaccuc ugcucagcga ggaacagauu aagaaucugg cgaaaaagca uuacacaggc     2220

uggggaaacu uaucacugaa acuuaucgac ggcauucagg auaaggggaa gaccaucuug     2280

gacuaucucg aaacgcagga uuacaaucaa aaccugaugc agcuaaugaa ugacgagaac     2340

aaaaguuucg aaaugcagau agagcaagca cgcgccggaa agguccaguu ugguaaaauc     2400

gauuauuccu ucguggagca gcuggcuggc agcccagcca uuaagcgagg gaucuggcag     2460

ucucuccaaa uuguugagga acugguggca uuucuuggaa aagaaaauau caagaagaua     2520

guaauugaga uggcccggga aaaccaguca aguaaacgua ccaagagcag acagaaacag     2580

aucgaggcuu uguaccaaaa ggucacaggc gaccugaaaa augagcagga aaagaucgau     2640

gugaugcaga acuucgacaa ugagaggguu uuucuuuacu aucuccagaa cgguaagugc     2700

augcacuccg gggaaaaacu ggagcuuucu caacugucgg aguacgaaau ugaucauauc     2760

auuccccaga cuuauauaaa ggacgauagc uucgacaaua aaguguuggu cacccgcaac     2820

gccaaucagc aaaagggcgg agaugugccu uccuacgacg uaaucgacaa aaugaaguca     2880

uauugggaga aacucaagga aaacaaccug auuagugaga agaaauuugc aaaucuaaca     2940

aagggcaaau ucgaugacca gcagcugucu ggguuuauca accggcagcu cguggaaacu     3000

agacaaauua ccaagaaugu ugcgaucaua cuggauaaaa ucuacaacga cgaggcuaag     3060

aaugagaagg aagucgccau uaucacacuu aaaagcgcau ugaccuccca guauaggcag     3120

ggaauuaucu acgugccaaa cgaugacuuc gaugagcuga aguuaguguc aaaaacuaau     3180

agccgacuca aggaagucaa acugcacgac gguuucuaca agguucgcga gauaaacgau     3240

uaucaccaug cccacgacgc uuaccuuaau aacguggugg ccacguaucu guacgaagca     3300

augcccgauc uacggccgcu cuuuguauau ggcgagcauu cuagagagau ucacaagagg     3360

uuccugggga aauacgcuac aacccguaag ggacaguuua aacaauugcu ggucgacaug     3420

caggacgaau auuggaugcg caaggauaaa gagggcaauu ucaucgacgg ugaaaucuua     3480

uggcggagag augaggugcu ccagauuguu aagaaggugc uggacuacag gaacauacag     3540

aucacuaaaa aguuagaaau gauggucacc gggggcgagg agaaauccuu uuaccaagau     3600

agucgauguc ggaaggacaa aacagccauu ccuuauaaga ccaauuggga ucccagcaaa     3660

uacggaggcu ucaagucucc acgcacugcg uaugccaucc cugugauuaa gaaaaacaag     3720

cccaaacugg aaccaguuuu gcucgugaau agauccaagg uagagacaga cgggcaguuu     3780

gauugcgaaa aauucaagga gcugaacccc acgcuaaaga uccugggaca gccuauaugg     3840

aaauaccagu cauuugagau guucgacggc accaagaggu auaucgcuag ugcaagcgaa     3900

auucacaaag guaaccaaau caaguuuucg caggagcaua ugaaauucau uuccuguugc     3960

cagaaguacg augaagucaa gaauaaacuu gccuuugacu ucgugaacga gucuaagcac     4020

cucuuccaag aaauccugac ugcuauaaaa gauaauauuc aucgguaucg auugaucgcc     4080

gaggggaucg aggugcacag aaaguuuagc aacaugcugg aaaauauuga gaaauuauca     4140

acacaggaac ucgucgacaa ccugcuugac gcaaucaaua uaaccagugu uggaacaacu     4200

aacaaugcgu ucggccgcgu gagguacaag gguuccuacc ugaacgagau uuggucugcc     4260

accuugaucc aucagagcgu aacgggccuc uaugaaaccc gugagaagcu gauuuga        4317


<210>  3371
<211>  4323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3371
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc ccugguggag       60

caggauuucg aaaauaaaca agguuugaua uuaggcaugg gguccagaau cauucccaug      120

gacgcuggaa ucauuggcaa guuugcagag gguucuucaa ucagucagac ugccgaaagg      180

acgggguacc ggagcauacg ccgacugcgu gagagacacc uacucaggcg ggaacgccug      240

cauagagucc uuaacauccu gaaauucuug ccugagcacu augcggcuga gauugauuuu      300

gaaaagaggu ucggacaguu ucucgccgag accgaaccaa aacuggcaug gaagaagucc      360

gccgagggca aauucgaguu ucuguuccag cggucuuuug acgaaauggu ugcugauuuc      420

aaggcaaaug gccaagacau caaaauuccg uacgauugga caaucuauua ccuccgcaag      480

aaagccuuaa cucagaagau aucgccccag gagcuggcuu ggauuauccu uaacuuuaau      540

cagaagcgag gguauuacca auugagagga gaagaggaag aggagaaccc uaauaaaaag      600

guggaguucu auucacugaa aauuguagac guggucgccg augaacagcc aaacaagaaa      660

ggugacaccu gguacagccu acaucucgaa aauggcuggg uguacaggcg gaguuccaag      720

aucccccugu augauuggaa agacaagguu cgugauuuua ucgugacaac cgacauuaac      780

gaggacgggu cugaaaaacu ugauaaagac ggagagguca agcgcagcuu cagagcgccu      840

aaagaggaug acuggacucu gauaaagaaa aagacagaac aggagaucga acaguucaau      900

caaaccgugg gcuccuacau uuaugaggca uugcucgaua acccaacgca gaagaucagg      960

gguaaacugg uacggacuau agaacgaaag uuuuacaaau cagaguuaaa gaaaauucug     1020

gagaagcaga ucgaacucca acccgagcuu uucaccgaag accuguauaa ugccugcgcu     1080

cgcgaguugu acagaaguaa cguugcccac cagcagcagc uaagcucuag ggauuuugug     1140

uaucuguucc ucaaugacau cauauuuuac caacggccuc ugcgcuccca gaagucacuu     1200

aucagcaacu guagucugga guucagaaca auuaaagaua aggacgggaa cggacagaua     1260

aucuacuuga aagcaauucc aaagucuaau ccguauuacc aggaauuuag ggucuggcaa     1320

uggcucuaua accugaaaau cuucacuaag gaggaugaca aggacgugac caaucaguuu     1380

aucaacaaag uggaagauuu agaggcucug uucgaauuuc uaaugaauca gaaggagguc     1440

aaccauacag acauucucac cuaccugaua gagcccaucg uuaaauccaa guauccuaau     1500

gccaaaggca aggcguucaa agaugaacuu aagaaggagu ugaaaaagua ccguuggaac     1560

uauguguacg acgccgauaa agacgaaagc aagucguacc caaugaauga gacgcgguau     1620

gaacugcgaa aaagacucga uaagguagag aacgugcccg cagacuuuau gacuaggaag     1680

auugagcagc accuguggca caucauuuac ucugucacag auaaaaucga guucgaaaag     1740

gcucuuaaau ccuucgccaa uaaguaugac cugcaucaag aaucauuugu ggagaacuuc     1800

aaacgcuuua agccuuucga uagugacuac ggcaccuaua gcgaaaaggc uauuaaaaag     1860

uugcucccac ugaugcgggu ugggaaauac ugguccuggg agggaaucga uaauaacacu     1920

aagaaaagaa uugguaagau cauuacaggc gaguaugacg aaaccaucaa gaauagggug     1980

cgcgagaaag ccauagacuu aacggaagag aaccacuuuc aggggcugca gcuauggcuc     2040

gcacaguaca uugucuacga ucgacauucu gaggccgaca ucgcuggaaa guggaacagc     2100

guggcggauc uggaacaaua ucuuucagag uucaaacagc acaguuugcg gaaucccauu     2160

guagaacagg uuaucaccga gacacugcgu guggucagag acauauggaa gcaauacggc     2220

aaaggugcaa aggauuauuu ugacgaaauc cacauugagc ucgggaggga gaugaaaaac     2280

acugccgagg aacgcaagcg gcuguccagc aucauuucug aaaaugagac cacaaacuua     2340

agaaucaggg cuauacugga ggaacuuaug uacgaugaga auauggaaaa cgugcgaccg     2400

uauucaccua ugcagcagga ggccuugaag auuuacgaag acggcguccu caguagcucc     2460

guggagaucc ccgaagauau cgagaaaauu ucuaagaaau cggagccaac uaagagcgaa     2520

auccagcgcu auaaacugug gcuagagcaa aaguaccggu ccccuuacac cggaagaccc     2580

auuccacugu caaaacucuu cacacccgca uaucagauag aacauaucau uccucaggcc     2640

agguuuuucg acgauggcuu uaguaauaaa guuaucugcg agucugaggu gaacgcucug     2700

aaggacaaaa ugcuuggguu ggaguucauu cagaauuacu auggacaaaa gguaaccacu     2760

agcuuuggug aagucacgau ccugacagaa gaugccuaca aagcauucgu gaaggagcac     2820

uaugacaaga accguuccaa acgcaauaag uuacuccugg aagagauacc agagaaaaug     2880

aucgaccggc agaugaacga uaccagauac auuucaaagu auaucagcaa aguucuuucu     2940

aaucugguga ggggcgaaaa ggacgaugag ggggucaacu ccaagaauau uaucccggga     3000

aacggcaaaa uaagugguac uuugaagcag gacugggggc ucaacgaugu guggaaugac     3060

cugauucuac cccgauucga acggaugaac cagcugacca auagcacaga uuuuaccgcg     3120

uggaacgaga aacaucaaaa guuccucccu acuaucccac uggaacuuuc uaaaggcuuu     3180

cagaagaagc gcaucgacca cagacaucac gcuuuggaug cccugauuau agcaugugcc     3240

acaagggacc acguaaauuu acucaacaau aaacaugcua agucccggaa cgagcgauac     3300

gaucugcagc acaaacuuag acauacgacc cccuggcaag acaaggaggg aaaagugcgc     3360

acuaaguuca cagaauuuaa aaagccuugg gagaccuuca cacaggacac uaagaaagaa     3420

cuggagaaga ucguugucuc auuuaaacag aaucuaaggg ugauuaacaa ggccaccaau     3480

uacuaugaaa agaucgugga uggcaagaag gucauguuca aacaggaggg gguuaacugg     3540

gcaauucgua agccaaugca caaagagacg gugaguggaa agaucgaccu cccccgggug     3600

aaaguaagca aggguaagau acugaccgcu acaagaaaau cguuggauac uuccuuuaau     3660

gccaagacca uccugucuau uacagacacu ggcauccaaa agauuuuacu caacuaccug     3720

aaggcgaaag ggaauccgga acuugccuuc agcccugagg gaaucgaaga gaugaaccag     3780

aacauaucac uguauaauga uggcaagcca caccagccca uuuugaaggu caggguguuu     3840

gagcagggua guaaauuccc ucucggggaa accggcaaca agacgacaaa auacguugag     3900

gcugcaaagg gaaccaaucu guucuuuggc guguaugaaa acaaucaagg gaaacgcucc     3960

uacaacacua ucccccuaaa ucuggucauc gagcgacaga agcagggacu uaacucugug     4020

ccagaaacaa augagaaagg ccauagacuc cuguucagcu uguccccuaa cgaccugguu     4080

uaugugccca uugaggguga aguauuugau aagaccaauc cagacgucaa ccggaucuac     4140

aagauagugu cauucagcgg gagucagaug uuuugcguga ggcaagaugu cgccacuucu     4200

auuguuuaca aauuagaguu cuccagccuc aauaagaugg aacgcaccau cgacggaauu     4260

augaucaaag agauauguau uaagcugaaa guggaucguc uuggcaacau cucgaaggcu     4320

uga                                                                   4323


<210>  3372
<211>  4323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3372
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc ccugguggag       60

caggauuucg aaaauaaaca agguuugaua uuaggcaugg gguccagaau cauucccaug      120

gacgcuggaa ucauuggcaa guuugcagag gguucuucaa ucagucagac ugccgaaagg      180

acgggguacc ggagcauacg ccgacugcgu gagagacacc uacucaggcg ggaacgccug      240

cauagagucc uuaacauccu gaaauucuug ccugagcacu augcggcuga gauugauuuu      300

gaaaagaggu ucggacaguu ucucgccgag accgaaccaa aacuggcaug gaagaagaau      360

gccgagggca aauucgaguu ucuguuccag cgguccuuug acgaaauggu ugcugauuuc      420

aaggcaaacg gccaagacau caaaauuccg uacgauugga caaucuauua ccuccgcaag      480

aaagccuuaa cucagaagau aucuccccag gagcuggcuu ggauuauccu uaauuuuaac      540

cagaagcgag gguauuacca auugagagga gaagaggaag aggagaaucc uaacaaaaag      600

guggaauuuu auucgcugaa aauuguagac guggucgccg augagcagcc aaauaagaaa      660

ggugacaccu gguacucacu acaucucgaa aacggcuggg uguacaggcg gagcaguaag      720

aucccccugu augauuggaa agacaagguu cgugauuuua ucgugacaac cgacauuaau      780

gaggacgggu ccgaaaaacu ugauaaagac ggagagguca agcgcucuuu cagagcgccu      840

aaagaggaug acuggacucu gauaaagaaa aagacagaac aggagaucga acaguucaac      900

caaaccgugg gcagcuacau uuaugaggca uugcucgaua auccaacgca gaagaucagg      960

gguaaacugg uacggacuau agaacgaaag uuuuacaaau ccgaguuaaa gaaaauucug     1020

gagaagcaga ucgaacucca acccgagcuu uucaccgaag accuguauaa cgccugcgcu     1080

cgcgaguugu acagaaggaa uguugcccac cagcagcagc uaucaagucg ggauuuugug     1140

uaucuguucc ucaacgacau cauauuuuac caacgcccuc ugagaagcca gaagucucuu     1200

aucuccaacu guucacugga guucaggaca auuaaagaua aggacgggaa uggacagaua     1260

aucuacuuga aagcaauucc aaagagcaac ccguauuacc aggaauuucg ugucuggcaa     1320

uggcucuaua aucugaaaau cuucacuaag gaggaugaca aggacgugac caaccaguuu     1380

aucaauaaag uggaagauuu agaggcucug uucgaauuuc uaaugaacca gaaggagguc     1440

aaucauacag acauucucac cuaccugaua gagcccaucg uuaaaaguaa guauccuaac     1500

gccaaaggca aggcguucaa agaugaacuu aagaaggagu ugaaaaagua ccgguggaau     1560

uauguguacg acgccgauaa agacgaaucu aaguccuacc caaugaacga gacgcgauau     1620

gaacugagaa aaaggcucga uaagguagag aaugugcccg cagacuuuau gacucgcaag     1680

auugagcagc accuguggca caucauuuac agcgucacag auaaaaucga guucgaaaag     1740

gcucuuaaau cguucgccaa caaguaugac cugcaucaag aaucuuuugu ggagaauuuc     1800

aaacgguuua agccuuucga uuccgacuac ggcaccuauu cagaaaaggc uauuaaaaag     1860

uugcucccac ugaugagagu ugggaaauac uggaguuggg auaucauuga cgacaagacu     1920

aaaacaagga ucggaaagau uaucaccggu gaguaugaug agacgauaaa gaaccgcgug     1980

cgagaaaaag ccauugacuu aaccgaggaa aaucacuuuc agggccugca gcuauggcuc     2040

gcacaguaca ucgucuacga ucggcauagc gaggccgaca uugcugggaa guggaacucc     2100

guggcggauc uggagcaaua ucuuucugag uucaaacagc acagcuugcg uaaccccauc     2160

guagaacagg uuauaacaga aacucugaga guggucaggg acaucuggaa gcaauacgga     2220

aaaggcgcaa aggauuauuu ugacgagauu cacaucgaac ucggucgcga gaugaaaaau     2280

accgccgagg aacggaagag acugucaagu auuaucuccg agaacgaaac aacuaauuua     2340

aggauacgag cuauucugga ggaacuuaug uacgaugaga acauggagaa ugugcgcccg     2400

uauagcccua ugcagcagga agccuugaag aucuacgagg acgggguccu cucuucaagu     2460

guggaaaucc ccgaggauau ugaaaagauc agcaagaaau ccgagccaac caagucugag     2520

auucagcggu auaaacugug gcuagaacaa aaguacagau cgccuuacac aggcaggccc     2580

auaccacuga gcaaacucuu cacccccgca uaucagaucg agcauauuau cccucaggcc     2640

cguuucuucg acgauggauu uuccaacaaa guuauuugcg aaucagaggu gaaugcucug     2700

aaggacaaaa ugcuuggcuu ggaguucauc cagaacuacu augggcaaaa gguaacuacg     2760

aguuuuggag aagucacaau acugaccgag gacgccuaca aagcauucgu gaaggaacac     2820

uaugauaaga aucgcucuaa acggaacaag uuacuccugg aggaaauccc agagaaaaug     2880

auugacagac agaugaauga uacuagguac aucagcaagu auauuuccaa aguucuuuca     2940

aaccuggugc gaggugagaa ggacgaugaa ggcgucaaua gcaagaacau cauaccgggg     3000

aauggaaaaa uuaccggcau cuugaagcag gacugggguc ucaacgaugu guggaacgac     3060

cugauccuac cccgguucga gcgcaugaau cagcugacaa acucuaccga uuuuacugcg     3120

uggaaugaaa aacaucaaaa guuccucccu uccguaccac uggagcuuag uaaaggguuu     3180

cagaagaaga gaauugacca caggcaucac gcuuuggaug cccugauaau cgcaugugcc     3240

acacgggacc acgugaacuu acucaauaac aaacaugcua agagccgaaa ugaaagauac     3300

gaccugcagc acaaacuucg ccauacgacc cccuggcaag auaaggaggg caaaguuagg     3360

acuaaguuca cagaguuuaa aaagccuugg gaaaccuuca cacaggacac uaagaaagag     3420

cuggaaaaga uugucgugaa cuuuaaacag aaucuacgug ugaucaacaa ggccaccaau     3480

uacuaugaga aaauugucga uggaaagaag guuauguuca aacaggaagg cgugaacugg     3540

gcaauccgga agccaaugca caaagagacg gugucuggga agauagaccu ccccagagua     3600

aaagucucca agggaaagau ccugaccgcu acaaggaaau cauuggauac caguuuuaau     3660

gccaagacua uucugagcau cacagacacu gguauucaaa agaucuuacu caacuaccug     3720

aaggcgaaag gcaauccgga gcuugccuuc ucgccugaag ggauagagga aaugaaccag     3780

aacauuuccc uguauaauga uggaaagcca caccagccca ucuugaaggu gcgcguuuuu     3840

gagcagggcu cuaaauuccc ucucggugag accgggaaca agacgacaaa auacguggaa     3900

gcugcaaagg gcaccaaucu guucuuugga gucuaugagg acaaccaagg caaacgaagc     3960

uacgauacua ucccccuaaa uauugugauc gaaagacaga agcaggggcu gaacucaguu     4020

ccagagacaa augaaaaagg acaucggcuu cucuucaguc uguccccuaa cgacuuggug     4080

uauguaccca uagagggcga ggucuuugau gaaaccaauc cagacgugaa caggauuuac     4140

aagguggucu cuuucagcgg uucccagaug uuuugcguuc gccaagaugu ggccacuuca     4200

aucguauaca agcuggaguu cagcaguuua aauaaaaugg aacguaccau ugacgggauc     4260

augauaaagg agauuuguau caaacucaag guggacagac ugggaaacau cucuaaagcu     4320

uga                                                                   4323


<210>  3373
<211>  4332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3373
augaucaagc ugcucuaccu ugagaaacug aagcaguaua ccaugaaaaa gauuuugggc       60

uuagaccugg ggacaagcuc cauaggaugg gcccuaguga acgaagcuga aaauucugau      120

gaagucucaa guaucauuaa acucgguguu agagugaacc cccugacugu agacgagaug      180

caaaauuucg aaaagggcaa gagcaucacg accaacgcag auaggacacu uaaacggucc      240

augcgccgaa aucugcagcg uuacaaguug agaagggacg cccucauuga gcugcugaaa      300

aagguggggu uuaucacuga uaaaaccaua cucucugagc agggaaaccg gacaaccuuc      360

gaaacuuauc gcuuaagagc gaaggcugcc gaggaagaga ucucgcugaa ggagcuugca      420

agggucuugc ugaugauuaa uaaaaagcgg ggcuacaaau caagccgcaa ggccaaaggu      480

gaagaggaag gggcucuaau cgacggaaug gagauugcaa agaaacucua ugaugaagac      540

cugacaccug gccagcuuug ccugcaauug cucgagagug gcaagaaguu uuucccagau      600

uuuuaccgau ccgaccugca ggaggaguuc aacaaaaucu gggauuuuca gaagcaguuc      660

aauuuauuuu cuuucuguga cguggccaaa gaggaaguua aggggaaaaa caagagccaa      720

accugggcua uacuggccaa guauuuugug gagacgaaaa aggucaaugu guggaacgaa      780

cacgaggcgc agacugagaa uagagaagag guauacaaac ucguuggacu uaagaggacc      840

gugaaaggug aggaacugaa gaaggaaaac uaccgguggc guguccaggc auugacagag      900

cagcuagauc uggagaaaau ugccguggug cuccaagaaa ucaauggcca gauuuccgcu      960

ucaagugggu aucugggagc caucagcgac cgcucuaagg uccuuuacuu caacagacag     1020

acuguuggcc aauaucagau ggcagagcug aacaaaaauc cgaacauguc cuugaggaau     1080

augguguuuu accggcagga cuaucucgau gaguucaacg uaaucuggga aaagcaggcu     1140

gaguuccaua aagaacugac ccccgaguua aagaaggaaa uucgagacau aaucauauuu     1200

uaccaacgca gacugaaauc acagaagggu cuaaucagcu ucugcgaguu ugaaaauagg     1260

gagguggucg uggagaaaga agggaagaaa cagacaaaga uaauuggaug uaaaguuauc     1320

ccucggaguc acccacucuu ccaggaguuu aagaucuggc aaacccugaa caauauugaa     1380

guguucgucg gcgagaagaa aggcaagcgc aaaaagucua acguguccag cgaucuuuuu     1440

ggggacacgg aagaugccuu gaucacugag ggaagaaggu uccuguauca ggaggagaaa     1500

aagauucucg cgaaggaacu gucgauuaaa aagggucuua caaaagccga aauccugcgu     1560

uugcucuuug agaaucccca ggaacuggac auuaacuuca agcagaucga uggcaaucaa     1620

accggguuug cauuauucuc ugcuuacucc aacaugaucg agaaauacgg auaugagccu     1680

cuggacuuua agaagccagc cgaagaucua auuucacagc ucaaaacugu auucacagac     1740

cugggcugga auaccgaucu uuuguucaua gaccuggaca aggagaacaa agaaugggag     1800

aagcagcccu acuuucggcu cuggcaucug uuauauaguu ucgaggguga uaauacgccu     1860

accgggaacg gcaaacugau cgaaaagauu augcagcuuu gcggaaucga gaaggaauac     1920

gcuauugagu uggccaacgu uagccuccaa gacgauuaug gcucccuguc ugcaaaagcc     1980

aucaagaaaa uacuacugua ccucaaggaa gggaaucagu augacauggc uugugaguac     2040

gcgggauacc gacacagcaa aucaagucug acaaaggagg aaaucgagaa caaagugcuu     2100

aaggauaagu uggaaauucu gccgaaaaau uccuuaagaa acccagucgu ggagaagauc     2160

cucaaucaga uggucaacgu gauuaauacu guuauggaag uguaugguag gcccgacgag     2220

auccgcguag agcuggcacg ggaacuuaaa aagagcgcca aagagagaga ggaacugacc     2280

aaggcugucg ccaagaacac aagggaacau gaggagauac gcaaaguguu gcaaucugaa     2340

uuuggcauga uuaauguuuc acgaaacgau aucauucggu acaagcucua ugaggaacug     2400

aaagacaaug gguacaagac ucuauauagu aaccaguaca ucccucguga gaaacuguuc     2460

agcaaggaga uagauaucga acacauuauc ccacagucca gacucuuuga cgauucucug     2520

ucgaauaaga cccuugagua uaaaagcauu aacaucgaaa agggaaauaa aacagcauac     2580

gacuucguga aggagaaaua cggcgaagag gguuugcagc aauaucugaa caggugcgag     2640

aacuuaugua aggauaagaa agccaagcuc cgcaaacuga agauggaacu uaaugagaua     2700

cccgacgggu uuauugaccg ggaucugaga aacacccagu acaucgcuaa aaaggcccua     2760

uccaugcuca cugaaaucug caggcgaguc guggcaacga caggcucaau uaccgacaag     2820

cugcgcgagg auuggcaguu gguagacgug augaaagaac ugaauuuacc uaaguaugag     2880

gcgcucggac ugguugaggu cuacgaagau aaagacggcc ggaagaucaa aagaauuaag     2940

gauuggacua aaaggaacga ccaccgucau cacgcuaugg augcccuuac cguggcauuc     3000

acaaaggacg uguuuauaca guauuucaau aacaagaaug ccgcuuggga gagugauucu     3060

aaagaacaua ccaacaucgu cgggauuaag acucgcuacu uugagagcgg aaaagccguu     3120

ccacccaucc cgcuggguca auuccgggug gaagcaaagu gucacuugga gaaucuccug     3180

guguccauua aagcuaagaa caagguaguc acaacgaaug ugaacugcau caaaaagaaa     3240

ggcgggaccu auaagaauac ucagcagaca ccuagaggac agcuacaccu ggagaccaua     3300

uacggcucac auaaccaaua cguuacaaaa aucgaaaagg ugaaugccag cuuugacgcg     3360

gucaagauug ccacuguguc uaaacaggau uauaggaacg cacuucucaa gcgacuggag     3420

guuuucggua acgaccccaa aaaggcuuuc accgggaaaa auuccuugga aaagaaccca     3480

aucuaccugg accgggccca gacgauucaa gugccugaua agguacagac cgucgaguua     3540

gagacagcuu auacuauccg caaacccgug gacccaaccc ucaaugugga uaaggucguu     3600

gacaaaaaga uaagacugau ucuugaacag aggcugaaag aguacggcgg agaugccaag     3660

aaagcauuug ugaacuugga cgaaaauccc aucuggcuca acgaggaaaa gggcaucagu     3720

auuaagcggg uaacaauacg agggaucagc aaagcgcagg cccugcacga uaagaaagac     3780

aaggauggaa auuucauucu agacgagacu ggcaaaagaa ucccugugga uuuugucaac     3840

accgguaaua accaucacgu ggcuauuuau cgcaagccag uucuggacaa gaaagggcaa     3900

gugaucuucg acgaugaggg aaauccgaug uacgaacuug acgaggaugu cgugcccuuu     3960

uucgaagccg uuacgagggc aaaccucggc auaccuauca uugacaagga uuauaaaaag     4020

ucugaggguu ggcaguuucu guucuccaug aaacagaaug aauacuuugu guucccaaac     4080

gagaagacag gcuuuaaucc caaggagguc gacuuguuag auccugacaa cuacgcuaug     4140

aucucaccaa aucuguuccg uguacagaaa auugggcuca acgauuauac cuuccggcau     4200

caccuggaaa cuacaaucca aaacgaccuu aaguuuauaa ccuggauuag auguggaaaa     4260

aguggcauca aggauaucgu gaaaguuagg auuaaucaca ucgggcagau agugagcguc     4320

ggagaguacu ga                                                         4332


<210>  3374
<211>  4332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3374
augaagaaaa uccugggccu cgaccuuggg accgccagca uuggauccgc uuacgugcug       60

gaggcagaaa agucugccga ucagacaaaa auagucuauu uggguacuag aaucauuuua      120

ucaagugcgg acgcugagaa cuucagcaag ggccuguccu gcucucccac gaccgauagg      180

acauuuaaac ggucgaugcg ccgacgucua caaagauaca agcagaggcg gucaagccuc      240

auccagcagu ucaagaaaau ugggaucaua acuaauaccg aacaaaucau ucgcgagccu      300

aucagugaaa cacacgagcu gcuuagaauu agguccaagg ccguuucuga gcaugugagc      360

cuggaagagu uugcacggau cuugcucacc auaaacaaac gccgaggaua uaaguccaau      420

agaaaaacuu caaagaguac agaagaggac ggcaagcugg uagaugccau ggcugugacc      480

aaagagcuga uuaagcucaa aaagacgcca ggucaguaca uguucgaaag guuuaaacgg      540

gggaaguaug cagagaucga cuucuacccg uuugauuuaa uuaacgaacu gaaacagauc      600

ugggaggucc agggacaaaa guaucccgac gugcuuacug aaggcuugcu gagcucucua      660

cucaagcaga aaaccggcaa ggccaaagag cugaucuacu ccaagucaga ucuuaauccu      720

gcugagguuc caauugacua ccagaaaggg aagaagcugc guaaaacaau auugaacaag      780

uauuucgccu acgaaagccg cagucucgcg gugacuaaua ucauucccgu cgagcaggca      840

aucaccgcca uaguggcuau uuguaaacaa aucaacaauu cuuccggaua ucugggugcc      900

aucggcgaua gaagcagggu auuauacgca aacaaucaga caauugggca guaucuguac      960

gaacggauca aggcuaaccc ucacgaccgc auucgaggag agguuuucua uagacaagau     1020

uaccucgacg aauuugacag gauaugggag ugccagcaga auuacuaucc agccauccuu     1080

accaacgauc ggaaacagua cauucgcaau agaaucaucu uuuaucaaag gccccugaag     1140

ucgaagaaau cugaguuggc guucugugaa uuugaguccc gugaagugga gcauaacggc     1200

aagaaauaca cggaaggucu acacgucgcc ccuaagucaa guccaguguu ccagcauuuc     1260

cggcuguggc agcgacucgg gcagauugag gugagcccgc ugagaaaaug ggacaagcaa     1320

uccaacgagc uuuuugaaca gcagaauaac gcagauaggg agggaauacu gaauacugaa     1380

gcuaagcaga aauuguucuc uuaucucaac cuguuagacc accugacaga uaaugagauc     1440

cuaaacauuc ucggcuacag ccccgacaag uggaaaauca auuuucgcga gauuaucggc     1500

aaccggacca auaaccugau gaugaaggcc uucuuuucaa ugaaagaaau gacugauggg     1560

gagcaaauag acugggaaaa gaaucuuaca aaaaucacca acggacagag uuugauuucc     1620

ccugagcaga ucgucgaucu gauuagacag uucuuugaaa gggaggguau cacgaccagc     1680

cuccuguauu ucgacccagc uacagauaau uuucgcgagc aaucucacua cucacuuugg     1740

caucuguugu acaguuauga agacgacaac agccccacug gcaccacauc ccucugcaag     1800

acucuggcca agcgauacgg gauaccucac gaguuauguc cacugcuagc aucucucacc     1860

uucgaacggc aguauggauc gcugagcaca aaagccaugc guaaacuuuu gcccuuuaug     1920

caggagaaga gauacgauga ggcuugcgaa cuggcgggcu auaggcauuc cgcauggauu     1980

accaaggagg aaaaugccaa acgcacucuc aagaacaagc ugccguuacu gcggaaaggu     2040

ucacuuagaa acccuguugu ggagaagauc uugaaucaaa ugacgcacgu aauuaacaca     2100

cacaucgcug aauacgggaa ucccgacgag auaaggaucg aaauggcccg agauaugcag     2160

gcaaacagua aacagcgcaa ggccaugacc gcugccauua cugaggcaac caaacggaau     2220

acacaguuca ucgaggaaau ucaaaaggag uuuggcuuca acccaucuaa gaaugaccuc     2280

aucaaauaua agcuguggaa agaacuagcg ccucugggaa acaagacccu cuacagcaau     2340

auauacauuc ccaaagagga gcuguucacu gcuaaguuug aaaucgagca uaucauucca     2400

agagcccuuu ucuaugauga cuccuuuuca aacaagacau uggaacugaa uagcgugaac     2460

aaauuaaagg gcaauaaaac ggcacucgau uaccuggaca cccuugggca ggagaaggcc     2520

aacgauuaua uccugagggu cgaagaguug aaaaagauug gagcuauaac ucaggccaaa     2580

uacaagaagc uccugauccg ugagaaagac cuaggugaug acuucacaaa ucgcgaucug     2640

accaacacac gguauauuau cagacacgca gcugacaggg accucacuaa cacccgauac     2700

auuauccggc augccgcgga uaugcugcuu caggugacgc ccucuauagu ucacaccaau     2760

ggcuccauca cagccauuuu gcgcagagac ugggggcugu uagauauccu caaggaacug     2820

aaccuuccua cuuaugagag uauugacagg aaaaucagcg auccagaaga gaagcugacc     2880

caaucugugg accggaaaga uggacaggcu auaaagauga uuaaggacug guccaaacga     2940

gaugaccaua gacaccacgc aauggaugcc cuaaucacag cucucacuac ccgcaagcau     3000

guccaguacc ugaauggcaa cucaggugcc gaagagacgg acaaagcaca gaaguuggag     3060

caacugaaag cguuaaggaa gcagcucauc gggagugaug gccacuuuau uccgcccaug     3120

caugacauac gugugcaggc caaggaagcu cugagccggg uaaucauuuc gacaaaaguu     3180

aagggaaaag ugaccacucc uaauacaaac aucauuuguu ccagagacaa gaccacuggc     3240

aggaaaaucu acguccgcca aaccacacuu acgccacgag gggcccugca cuuggauacc     3300

aaguauggaa uaaauagaua cuaucaggag cgggaagugu cuaucgguag caagcucacu     3360

caggaggaaa uuaggcuggu cucaaguccc cacauccagu ccauucuacu ggagcgccuu     3420

cgugcauucg acaacaaucc uaaaucugcu uuuacaggca gaaacagcgu ggauaagaaa     3480

ccacucgagg acaagaauaa aaagcugguu uacaccgaua agaaagugaa gcaaacaauc     3540

uuggccaacc gguucguaaa aagggaccgc gucaauucca agcuggauuu agacaacgug     3600

auagauacug ggauuaaacg aauccucgaa agacggcuga agcaguaugg aggcaaauac     3660

gagaccgcau uuucagaccu ugaaaauaac cccaucuggc ugaacgagga gaaggguauu     3720

gccaucaaga gcguuaggau acgcgggaaa aauaacacga uuacaaucag acagaccaau     3780

acuugcagga uuaaguaugu ggcuaacgau aauaaccauc acuuggcggu cuacgcccuc     3840

ccggacggca guaucaaaga cgauauagug ucuuucuuug acgcaguaga acggucccgu     3900

aagcgccugc cuauuaucga uaaacauaag aaucagagcc uaggauggaa guaccuguuc     3960

ucgaucaaac aaaacgagua uuuucuucuc ccacaacagg uggaaaagau ugacaucgau     4020

accggcgaga uuucaaugca gacaacuuuc ucucccaaag accugaguca cgaauacaua     4080

augaauccug auaacuaugc ugaguuggcc ucccauaucu uccgaguuca gagcauuucu     4140

aagacacccc aggggaauag agacgucagg aucuacuuau uuaaacagca guauaucagc     4200

ggaucccaaa aggauucacu gcaccucaag ggcauuaccc accggcagau aaguagcgcc     4260

aaagaccugg cugagcuugu gaagauccgc gugaaccauc uggguagaau ugucucuguu     4320

ggggaaaaau ga                                                         4332


<210>  3375
<211>  4341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3375
auggugaaga acauccuggg ccucgaccuu gggaccaaua gcgucggaug ggcccugauu       60

aaacaggauu ucgagaacaa gcaaggugac auauugggca ugggguccag aaucauuccc      120

augucucagg auguuuuagg agacuuuggc aaagguaauu caaucaguca gacagcugaa      180

aggacuaagu accggagcgu gcgccgacug cgugagagau uccuacucag gcgggaacgc      240

cugcacagag uacuuaacgu gcuggauuuu cuuccugagc auuaugcauc ccagauugac      300

uucaauaaaa gguuugggaa guucaaggaa gagacggagc caaaacucgc cuacucuaac      360

gauggauuua ucuucaagaa aucguuugaa gagaugauau cagacuucaa gacccaccaa      420

ccggaacugc uggagaauaa caaaaagguc cccuaugauu ggacaaucua cuaucuccgg      480

aauaaggcgu uaagccauaa aauugagaag gaagaacugg cuuggauccu uuugaacuuu      540

aaucagaaac gcggcuacua ucagcugcga ggcgaagagg aagaggagaa cccuaauaag      600

cuaauugagu ucuacagucu caaaaucgug gacguucugg ccgaugagcc acagaagggg      660

aaauccgaca uaugguacuc ucuuauucug gaaaacggau ggaucuauag aaggagcucc      720

aagauucccu ugcucgauug gaaggguaaa acucgggacu uuaucgugac cacagaccug      780

aaugaugacg gcucagucaa gaccgauaaa gaggggaacg aaaagcguag uuuccgcgca      840

ccuggagaga acgacuggac uuuagugaaa aagaagacag agcaagaaau cgauaauagc      900

gacaaaaccg uaggcgccua cauuuaugag gcucugcucc agaacccaaa gcagaaaaua      960

aaggguaaac uuguuagaac gaucgaaagg aaguucuaca aggcagagcu gaaacagauu     1020

uuggaaaagc aaauggaguu ucaccccgag cuacggcagg augaccugua uucugauugc     1080

gugcgagaac ucuacaaaaa uaacgaggcc caucagcuga cucuuuccaa gaaagacuuc     1140

guccaccugu uuauggaaga uaucauauuc uaucaacgcc cuuugagauc acaaaagagc     1200

aguauuucua auuguacccu cgaguuuagg aaguacaaag acgagaacgg gacagaacac     1260

auccaguacc ugaaggcuuu accaaaaucc aauccguauu accaggaguu ccggaucugg     1320

caguggaugu auaaccugaa uauuuacaag aaagaagacg auacuaacgu gaccaaggag     1380

uuucuaaaua gcaaagccga cuucgaaucg cucuuugagu uccugaacaa uaagaaggag     1440

guggaucaga aagcgcuuuu gaagcauuuu aaaaucaacg acaagacaca ccgcuggaaa     1500

uaugucgaag auaagaagua ccccugcaau gagaccaaaa cgaugauuuc ugaaagacug     1560

aagaaaguug agaacauauc cgacgauuuc cucacuaggg aaauggagca gaagaucugg     1620

cauauuaucu auucagugaa cgacaaagua aaguacgaga aggcacugaa aaguuuugcc     1680

gaaaagaaua accuugauga gagcuccuuc uucgaggcuu uuaaaaaguu cccuccauuu     1740

aaaucugaau acggagccuu cagcgaaaag gcaaucaaga aacuguugcc ccucaugcgu     1800

cugggcaagu auugggacga ugacgagauu uuaaaauacu cagauauaua uuuuaagaau     1860

aucgaguacc ugcuaggcgu gcucaaacag aaggacaaaa acauuaguga cgauaaucgg     1920

caaaacugga auaaguccau caacaauaag cugcgagagg aacuugaaau uuuccagaac     1980

gcugagaucg agagcuuuca gggguugaga cugcacauag cccaguauau ccucuacgga     2040

aggcacucug aagcggccau gcuggguaaa uggaacucag cagacgauuu agaggaguuc     2100

cugaaggaau uuaaacaaca uagucuucgc aauccuauug ucgaacaggu gaucacagag     2160

accuugcggg uugugaagga cauuuggguc aaauauggca auggggcuaa ggauuucuuu     2220

aacgagaucc acauagaacu cggaagagag augaagcugc cagccgaaga caggaaaaag     2280

auuacuaauc agaucagcga gaacgaaaac acaaaucuac gcauuaaagc ucugcucgcc     2340

gagaugaugg uggauuccuc uguagagaac guucgacccu ucucgccgau gcagcaagaa     2400

auccugaaga uauacgagga cggcgugcuu aauagcgaua ucgaaauuga ggacgauauc     2460

uugaaaauuu ccaagaccgc acagccuuca cccagugacc ugaagcggua caaauuaugg     2520

cucgaacaga aguauaaauc uccauacacg ggucaaauca uaccucugaa caaguuguuu     2580

acccccgagu augagauuga acauaucauc ccacagagcc guuacuucga ugacuccuuc     2640

ucaaauaaag ucauuuguga ggccgcugug aacaagcuga aggacaauuu uaucggguug     2700

ggcuucauua aacaguuugg aggcacaaua gucgaaugcg ggaacggaaa gagcgugacu     2760

guucucaaag aggaucagua ugaggacuuc gugaagaaac acuacgcgaa uaacagaucu     2820

aagaggaaua agcugcuacu ggaagauauc cccgagaaaa ugauugaacg ccaaaugaac     2880

gacacccggu auaucuccaa guacauuagu gguguacuca gcaauaucgu cagaacagag     2940

gauggcacug acgauggggu gaacucuaaa aauauaguuc cuggaaacgg caagaucacc     3000

aacacacuga aacaggacug gggucuuaau gauauuugga acgacuugau ccugccaagg     3060

uuugaacgaa ugaaucaguu aaccaacucc acugauuuca cggcauggaa ugagaaccau     3120

cagaaguuuc ucccgacagu gcccgucgag uucucaaaag gguuuaguaa gaagcgcauu     3180

gaccaccggc accaugcccu ggacgcucuu gugaucgccu gugcaaccaa agaucacgua     3240

aaucugcuaa acaaucaaag cgccaagucg gacacuaaaa gacaugaucu caagaggaaa     3300

cugaugaagu ucgaaaaggu ggcuuacaac cacccucaga ccggcgagcg uauagaacgc     3360

gagguuccaa aacaguuuuu gaagcccugg gaaacauuca ccgucgaggc caaaaaugga     3420

cuggagacua uuaucguguc cuucaagcag aacuuacggg ugaucaauaa agcaacaaac     3480

cacuaugaaa aguacgucga gaaggacggc gcgaaaguua aggggagagu ggaacaagcu     3540

ggaacgaauu gggccauuag gaaaccuaug cauaaggaga ccgugucugg uaaaauagau     3600

cucccacgaa ucaagguacc caagggcaaa auucugacug caacacggaa gaaccuugac     3660

accagcuuug aucugaaaac aaucggguca auuacugaca ccggaaucca gaagauauug     3720

aaaaauuauc uccaguacaa ggauaacaac ccggaacugg ccuucagucc ugagggcauc     3780

gaggacauga auaaaaacau ugaaaaguuu aaugauggua agguccacca accaaucauu     3840

aaagugcgca ucuucgagcu aggguccaag uuuccccugg gccagucugg aaacaaaaag     3900

gacaaauaug uugaagcugc caagggcacg aaucuuuucu uugcagugua cgaggauaag     3960

aacgggaaaa gaagcuauga gaccauaccu cucaaugaag ucauugagag gcagaagcag     4020

ggacugacag ugguugacuu gaaaggcgug aacgacuucu accugucccc caaugauuua     4080

guauacgucc caucagaaga cgagcucgaa aacgugagca ugaucgauua uaauaagcug     4140

aguaacgaga aaguguacaa ggucguuucu uuuacuggua aucaaaucuu cuuugugcgg     4200

caggacguag cuaccuccau ugugaacaag gccgaguuca gcucgcuuaa uaaaauggaa     4260

cgagcgaucg ggggaacaau gauaaaggag gucuuuguga aaauuaagau cgauagacug     4320

ggcaacauuu caaaaguuug a                                               4341


<210>  3376
<211>  4347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3376
augaagcaca uccugggccu cgaccuuggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augauaacga acaauccgag auaguugacg uggguguaag agugaauccc      120

cugacaacug augaaaagac gaacuucgag aaaggcaagc cuaucucuac caaugcagac      180

aggacauuga aacggucagc ccgccgaaac uuacagcguu acaagcugag aaggaaggaa      240

cuaauugagc uccugaaaga gcaucagcuu aucagugaaa auacuauucu gaccgagaac      300

gggaagggaa caaccuuuca gacuuuggaa cuccgggcga aaagcgcuaa ggagaaaauc      360

ucccugggcg agcuggcccg cgucuuccuc acaauaaaua agaagagagg uuauaaaucu      420

ucgaggaagg ccaaaaacgg ggaugaagga caauuaaucg acggcaugga gauugcaaag      480

cggcuguacg aagagaaucu uaccacgggc caguugucac ugcagcuacu caaagaaggg      540

aagaaaagcc ugccagauua uuaccgcagu gaccuucagu uugagcugga uaaggugugg      600

aacuuccaaa agcaguccca cucugacauc uugacugaug aguuuuaugc ucagcucaaa      660

ggaaaggguc agcgagcaac cagcgcccug uucuuagcua aauaccaaau uuauacagcc      720

gaaaauaagg gcuccagaga ggacaaaaag cugcaggcgu accaguggag gguugaugca      780

cucucaaagg aacuuacucg ggaggaacug gccuuuguga ucaccgagau aaacaauaac      840

uugaacaaua guagcgggua ccuaggagcu auuucugacc guuccaaaga gcuguauuuc      900

aacaaugaaa cagucggcga guaccucugg aagcaaaucg ccgcaaaccc gcauaauuca      960

cugaaaaacc agguguuuua ucgccaggac uaccuugaug aguucgagca gauuuggaag     1020

acccaaagcg cuuuuuaucc cgaaauccug acggacgagu ugaaaaagga gcucagagau     1080

guaguuaucu ucuaccagag gaagcugaaa agucagaagu cuuuaguguc cuuuugcgag     1140

uucgaaaaua aagaaauuga gauaaagaac gaggacggua cuacaaccaa aaaggucauc     1200

gggcugaagg uggccccuaa aagcucgcca cuauuccagg aauuuaagau uuggcaaaau     1260

cucaacaaug ugcugauccg gaaaaaggga ucuaaaacua agaaagucgc gaagaaccag     1320

caggccguuc uuuucgagga agagaagaca auauccgaau uggaucugga ggcaaaacag     1380

gcucucuuua augagcugaa ccuuaagggc aaucugaaag ccgacuauug uuuggaaauu     1440

cucggcuacu cacccaagga gugggaaaug aacuacagua uccuggaggg gaaccgaacc     1500

aauaaggcuu uauaugaagc cuaccugaag aucacggauc uagagggaua ugacgcaaag     1560

aaccaccuca aagugaagcu gaauaaagau gagguagaac uugacgauuu gaaggugccu     1620

gccagcgaga uuaaagacau gguccaagcu aucuucaagg aagcggguau uaacaccgac     1680

auacugcauu uuaaugcaga gcucgauggc aaggacuucg aaaaccaggc cuccuaccag     1740

cuguggcacu uacuguauuc uuacgagggg gaugacagca aaucaggaaa ugagcuuuug     1800

uauaagcucc uggaaacaaa auuuggcuuc aagaaagagu acgcucagau ccuagccaac     1860

gugagucugu ccgaugacua cgguaaucuc agcacuaagg caaugcgcga aauuaucacc     1920

caccugaagc aaggguuucc auucacaggc agaaaagaua ggcccgacga gccuucugcc     1980

ugcgaguuug cuggauauaa gcauucaaaa cacagucuua cuaaggaaga gaacgaaaaa     2040

cggguuuuga aggauagccu ggagguguua aagaaaaauu cccuccgcca gccagucgug     2100

gaaaagaucc ugaaccagau gauuaaucuu guaaacgccc uguugaaaaa guacagaacc     2160

gagaauccga acuucaaauu ugacgagaua aggaucgaac ucgcacguga gcugaagaaa     2220

aaggcggagg aacgggcuaa caugacagcc aauauuaaca aggcaaaaac cgaacaugag     2280

aagaucauua aaauccuaaa gaaugaguuc ggccugccca acccuacucg aaaugauaua     2340

aucagauaua ggcucuacga ggaacugaaa gacaacgggu auaaggaucu uuacaaggga     2400

gacuauauuc cacgcgaaaa auuguucucu aaggagaucg acauugaaca caucauaccc     2460

aaagcccggg uuuuugauga cucguucagc aauaagacga uuacauacag aaaaaccaac     2520

cuggagaagg gugagaggac ugcuuacgau uauaucgaau ccuuaggcga gaagcaacuc     2580

gaagaguuuc ugucacgcau ugagaaucuu uacgaaaucg ggaaaccuag ucgacugcac     2640

aaccggcgua auccacuaaa gaaaguggag gaaaagggaa uaucugacag caaauauaag     2700

aagcuccuga aaaaggaguc cgaaaucggc gaugguuucg ucgagagaga cuugagggag     2760

acccaguaca uugccaaaaa ggcaaaagaa auccuguuac agauuacacg cucagugcuc     2820

agcaccucug ggggcaucac ugaucggcug agagaggacu gggaucuuau aaacgucaug     2880

aaggaacuga auuugcccaa auauagggcu cucggacuga caaagaugga ggaccgaaag     2940

uacggccagc aaguggaagu uauuacggau uggaccaaac gcaacgaccu acggcaucac     3000

gccauggaug cgcugacugu ggccuuuaca aagcauaauc acauccagua ucuuaacuac     3060

cucaacgcuc gcaaaaauga gaagcauaaa gagcacaaca auaucauggc aauucagcag     3120

cuggaaacca ucaagguaac agacgagaac gggaaugaaa agaggauuuu caaagccccg     3180

augccuaacu uucgucaagu cgcuaaggcc uucuuggaga augugcugau aucccacaaa     3240

gcaaagaaca aaguugugac uaagaauaag aacaaaauca guggaaauaa caagguccag     3300

gaaguguuaa cccccagagg ucagcuccau aaagagacga uuuacggcaa guaucaguac     3360

uauguacaaa aagaggaaaa gaucggggga aaguuugacc uggagaccau ugaaaaagug     3420

gcgaauccac uuuaccagag ccuguugaag aaacggcucg aggagaacgg cggugauccu     3480

aagaaagccu ucacagggaa aaauucucug gcuaaaaacc ccaucuaucu agacgaagcc     3540

cagacugagc aacugccaga aaagguuaaa cuugucuggc ucgaaaauuc cuuuucaaua     3600

agaaaagaua ucacccccga aaacuucaag gacgagaaac ugauugauaa gaucuuggac     3660

gagggcguga aaaggauuuu aaaggaacga cugcagaguu uuggaaacga ugcaaagaag     3720

gcuuucagcg accucgagaa gaauccuauc uggcugaaca aagaaaaggg cauagccauu     3780

aaacggguga caaucucggg ggucgcaaau gccgagccac uucacacuaa gaaggaucau     3840

cugggaaaag aaaucuugga caaggagggc aaaccgauuc ccguugauuu cauauccacc     3900

gguucuaacc accacguggc uguguaccgc gacgagaagg ggaaucuaca ggaaaaagua     3960

gucagcgugu uugaggcggu ugugagagcc aaccagggac ugccuaucau ugacaagacg     4020

uauaaucaau accucggcug ggaguuccug uuuucaauga agcagaacga aauguucguc     4080

uuuccaaaug aaaguacagg uuucaacccc aaugauaucg accuuuuaga uccuaaaaac     4140

aagaaaauua ucuccccaaa ucuguuuagg gugcagaagc ucgcaaccgg cgacuacugg     4200

uuccggcauc acuuggagac uacaguugag accaaaucug cuacuaagga uaugaccuau     4260

aaacgacuga gccucaacca uauaaagaau aucauuaagg ugagaaucaa ccaccugggg     4320

gacauuguau ccgucggaga auacuga                                         4347


<210>  3377
<211>  4353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3377
augaagcaca uccugggccu cgaccuuggg agcaacucca uuggaugggc cuucgugcag       60

caagauuuug agaauaaaca ggguaagaua aucgcuaccg gcucuagaau uauccccaug      120

gaccagggga uucugggaga uuucgaaagg ggcaacacag ucucacagac ugcagagcgg      180

acgaccuacc gcaauacacg acguuugaga gaaaggcauu uacugcggcg cgagagacua      240

cacaggguuc uccauauccu ggguuuucuu ccuccacacu augacaguca aauagauuuc      300

acuaaacggu acgggaaguu uaucgacaac gccgaaccga aaauugcgua uaauaacgga      360

aauuucaucu uuaugaacag cuucaaugag augauugagg auuuuaagaa guaccagccc      420

cagcuguucu auaaaaaguc caacggcgaa gagucuaaaa ucccuuacga cuggaccaua      480

uauuacuugc gcaagaaagc ucucucgcag aagauuacac aacaggaacu ggccuggcug      540

auccuccauu uuaaucagaa gcgaggcuac uaucaguuaa gaggggagga ggaaaccgag      600

aacccaaaua aagaaguggc auuccacuca cuggagguag uggaugucga agccgaggcu      660

cccaacaaga aaggagagau uugguacacu aucaggcuug aaaaugguug gaucuaucgg      720

cguacaagca agauuccuuu ggacgauugg aaaggcaaga cccgcgacuu uauagugacg      780

acugaucuga acgacgacgg gaccaucaaa cuagauaagg agggaaagga gaaaagaagu      840

uucagggcac cagacgagaa cgauuggaau cuccugaaga aaaagacaga acacgacauu      900

gauuucuccg gcaaaacugu uggugccuac aucuaugacc aucuucugca aaaccccaau      960

cagaagauaa aggggaaauu ggugcggacc auugagcgaa aguacuauaa agaugagcuc     1020

aaggcuaucc uggccaaaca gaucgcguua caaccugaac uguuuacaga gacccuccuu     1080

gcagacugca uucgcgaacu guacagaaag aacgagacgc agcagcagaa uuugcuaucu     1140

aaggauuucc ugcaccucuu ugucgaagac aucauuuucu accaaaggcc acugcggagc     1200

aaaaagucca cuauagccaa cuguacacuu gagaaacgcu cauauaucga uaaggagacc     1260

aaugaaagaa aagacacucc gauuaaggug ugcgcuaaga guaaccccua cuaucaggag     1320

uuuaggguac ugcagugguu gcagaaucuc cguaucuacg aaaucgagac agaccgggag     1380

guuaccgccg aguucauuga aacggcacaa gauaacgaag cucuguuuaa uuucuuaaug     1440

agccagaaag agauagacaa cgaaucucug cuaaaguauu uucucuucgc caaaaauccu     1500

cagaucaagg auaaaguggc gaaguccgag cugaaaaagu ggcuuaccac auaccgaugg     1560

aacuaugucu acgacgccgc agauaauuca agcaagaaau acccaaugaa cgagacugga     1620

uaugaauugc agcaauaccu gcagaaggug gagggccucc ccgcuaacuu ucugaccuuc     1680

gacauuacau auagacuuug gcaucuguug uacaguguga aagauaaggu cgaauuugag     1740

aaagcccuca agaaguucgc ucaggaauau cagcuggacg aggccucuuu cguugauucc     1800

uuuaaaaauu ucaagccuua cccaaacgag uauggcagcu uuucggaaaa agcaaucaag     1860

aaauuacugc cccuaaugag guucgggaau cacugggacu uuaaccgcau ugaugugucu     1920

acucaaaagc ggaucgacga ucucauaaau ggaguagcca acgacgagau cagaaccauu     1980

aucagggaaa aggcugagaa auaccagcuu aauuccgaaa cauguuucca gggucugccg     2040

uuguggcugg cgcaauacgu ggucuauaac cgccacgcag aggccgcuga cauugagaag     2100

uggaccucag ugaaugaucu cgaagccuac cuggaggacu uuaaacagca uaguuuacga     2160

aacccuaucg uugaacaggu gauaacugag acgcugcggg ucgugcguga uauuuggcag     2220

agauauggcg ggggacaagc aaauuucuuu gacgagaucc acauugaacu uggcagggag     2280

uugaagaaaa cagccgaaga acgcaagcag cucagcaacc ggaaucagga aaacgagaau     2340

accaaccuga gaaucaagaa acuacuggcu gagcucaagg aagaugccuc cauacagaac     2400

guaaggcccu acucuccagu ucaacaggag cugcuuaaaa ucuaugaaga ggguguguuc     2460

agcgcaacug acgaagucga ggaggauauu cagaagaucc gcaaaaaggc ggaaccuacc     2520

acacccgaga uucagcgaua caaauugugg cuggaacaaa aguauaaguc accauacacc     2580

ggggagguga ucaguuuaac uaaacucuuu acagaagagu acgagauaga acauauuauc     2640

ccccaguccc gguauuacga cgauagccug ucuaauaagg ucaucugcga gucagcugug     2700

aacaaacuua agacgaauca gcugggcuug gaguucauuc aaaaccacca uggagaaaaa     2760

guuagaauca ccgccaauaa ggaggugacu auuuuuacag aggaacagua uaaggaguuc     2820

gucaaaaagc acuacgcaaa caauagugcc aaacagcaga agcuccugcu aaacgaaaua     2880

ccugagaaaa ugguagcuag gcaacugaau gacacccguu auaucagcaa guacauuucc     2940

gaaauccucu cuaagauugu gcgcucggau gagaaagacg aaggcguuaa cagcaagaau     3000

aucauaccau guacagggaa aaucacuacc gcccugaagc aggauugggg acuuaacgac     3060

guguggaaug aguugauucu gccgcgguuc gaaagaauga accaguuaac gcagacccaa     3120

cucuuuacau ccuauaauga cagguuccag aaaaaccugc ccacuguccc uaucgaguac     3180

ucacaggguu uucagaagaa gcgaauugau caccggcauc acgcaaugga cgcucuugug     3240

aucgccugcg cgacccgcga acauauaaac uacaugaaua accaacacgc ccuggauaaa     3300

aguaaggaca aaaaggagaa acagcagucu agaauugauc uaaggacaau ccucugugag     3360

aagaagaaua acaauuggca guucaaaaag ccauggagca cuuuuaccca agaagcucag     3420

gaggcacugg aacacaucgu aguguccuuc aaacagaacu ugcggguuau uaauaaggcc     3480

acgaacuauu acgagaaaug ggaagagaag aauggcgucc uggugaaaaa gaaggugaaa     3540

caagagggga caaacugggc uauacgaaag cccuuacaua aagaaaccgu cucaggaaag     3600

aucguucucg acagaaaaau uggcaaggau aagauccuga cugccacacg caaaagcgug     3660

gacauuaccu uuacugagaa gaucauaucu uccaucaccg auacagguau ucagaaaauc     3720

cuucugaauu auuugaagua caaagggagu ccugaagugg cauucagcgc ggagggccuc     3780

gaggaacuga acaagaauau ugcccaguau aacgacggaa agaaacacca gccaaucuac     3840

aagguaagga uauuugagga aggcucuaaa uucccccuag gggagacggg agcuaaggcc     3900

accaaauaug ucgaagcagc uaaggguacu aaucuguuuu ucggcgugua ccaagggaag     3960

ggaaaacgua cauacgccac cauuccgcuu aacgagguua ucgagcggca gaagcagggc     4020

cucccuuccg ugccagaaag aaaugagaaa ggugaacccc uguuguucuc acugaguccu     4080

aacgauuuag ucuaugugcc cauggagggg gaaaucgcag agacaauuga cuuuaacaau     4140

cucagcaagg agcagaaaga aaggaucuac aagacuguuu cguucaccgg caaccaaugc     4200

uuuuucgugc aggaggccgu agcuacgcca auagucaaua aaauugaaua uuccccucug     4260

aacaagaugg agcgcgacau ccuuggaaca augauuaagg aaguguguau caaacugaag     4320

guggaucgau ugggcaauau aaccaaagcg uga                                  4353


<210>  3378
<211>  4353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3378
augaagagaa uccugggccu cgaccuuggg agcaacucca uuggaugggc cuucguggag       60

caggauuuug aaaauaaaca agguaagaua aucgcuaccg gcucuaggau uauccccaug      120

gaccagggga uucugggaga uuucgagaaa ggcaacacag ucucacagac ugcagaacgg      180

acgaccuacc gcaguaugcg acguuugaga gagaggcacu uacugcggcg cgaaagacua      240

cauaggguuc uccacauccu ggguuuucuu ccuccacauu augacgccca gauagauuuc      300

acaaagcggu acgggaaauu uaucgacaau gcggagccga agauugcuua uaacaaugga      360

aacuucaucu uuaugaauag cuucaacgag augauugaag auuuuaagaa auaccaaccc      420

cagcuguucu auaagaaauc caauggcgag gaaucuaaga ucccuuacga cuggacuaua      480

uauuacuugc gcaaaaaggu gcucucgcag aagauuaccc agcaagagcu ggccuggcug      540

auccuccacu uuaaccagaa acgaggcuac uaucaguuaa gaggggagga agagacagaa      600

aauccaaaca aggagguagc auuccauuca cugaaagugg ucgaugugga agccgaggcu      660

cccaauaaga aaggagagau uugguacacc aucaggcuug aaaacgguug gaucuaucgg      720

cguacuagca agauuccuuu ggacgauugg aaaggcaaga cacgcgacuu uauaguuacc      780

acggaucuga augacgacgg gacuaucaag guggauaaag agggaaagga gaaaagaagu      840

uucagggcac cagacgagaa cgauuggaac cuacucaaga aaaagaccga acacgacauu      900

gauuucuccg gcaagacagu cggugccuac aucuaugacc aucugcuuca gaaucccaac      960

cagaaaauaa aggggaaacu ggugcggacu auugagcgaa aguacuauaa agaugaguug     1020

aaggcuaucc ucgccaagca aaucgcgcug cagccugaau uauuuaccga gacacugcuc     1080

gcagacugca uucgcgaacu uuacagaaag aaugagaccc aacagcagaa ccuguugucu     1140

aaggauuucc uacaccuguu uguagaagac aucauuuucu accagaggcc acuccggagc     1200

aaaaagucca cgauagccaa uuguacucug gagaaacgcu cauauaucga uaaggagaca     1260

aacgaaagaa aggacaccuu uauuaaaguu ugcgcuaaga guaauccgua cuaucaagag     1320

uucagggugc uucaguggcu gcagaacuug cguaucuacg aaaucgagac ugaccgggag     1380

gucacagccg aauuuauuga gaccgcagaa gauuaugagg cucucuucaa uuuucugaug     1440

agccagaaag aaauagacaa cgagggauua cugaaguacu uccuauuugc caaaaauccc     1500

caaaucaagg auaaaguggc gaagucugag cucaagaaau ggcugacgac cuaucgaugg     1560

aacuacgugu acgacgccgc agauaauucc ucaaagaaau auccuaugaa cgaaacaggc     1620

uacgaccuuc agagauauuu gcagaagguc gagggccugc cagcuaauuu ccucacuuuu     1680

gauauuaccu acaggcugug gcaccuucug uauagcguua aagacaaggu ggaguucgaa     1740

aaggccuuga aaaaguucgc ucaggaauac caacucgaug aggccaguuu uguagacucu     1800

uucaaaaacu uuaagcccua uccuaacgag uacggguccu ucagcgaaaa agcaaucaag     1860

aagcuguuac cacugaugcg cuuuggaaau cauugggauu ucaaccggau ugacgugucg     1920

acacagaaaa gaaucgauga ccuaauaaau ggugucgcca acgacgagau caggacuauu     1980

auccgcgaaa aggcugagaa auaccagcuc aauucugaau ccuguuuuca gggccugccc     2040

cuuugguugg cgcaauaugu gguuuacaac cgacacgcag aggccaccga uauugagaag     2100

uggacaucag ugagugaccu ggaaaccuau cucgaggauu ucaaacagca cagccugcgg     2160

aauccgaucg ucgaacaggu gguaacugag acguuacgug uugugagaga cauauggcaa     2220

caguacgggg gaggccaggc uaacuuuuuc gaugagauuc auaucgaacu ggguagggag     2280

cuuaagaaga cagccgaaga acgcaaacag uuguccaauc ggaaccaaga aaaugagaac     2340

accaaucuca gaauuaagaa acugcuagca gagcugaagg aagacgccuc uauccagaac     2400

gucaggccuu auagccccgu gcagcaggag cuccugaaaa uauacgaaga gggggucuuu     2460

ucaaguacug aucaagugga agaggacauc cagaagauuc gcaaaaaggc ugagccaacc     2520

acaccugaaa uccagcgaua uaaacuuugg uuggagcaga aauacaaguc ccccuacacc     2580

ggcgaaguua uuagccugac uaaauuauuc acagaggaau augagaucga gcacauaauu     2640

ccacaaucuc gguacuauga ugacucacuc aguaauaagg ugaucugcga aagcgccgua     2700

aacaaacuga agacgaacca gcuuggacug gaguuuaucc agaaucauca cggcgaaaag     2760

gucagaauua ccgggaacaa agaggugacu aucuucacag aggaacaaua caaggaguuc     2820

guuaaaaagc auuaugcaaa uaacuccgcg aaacagcaga aguugcuccu gaaugaaauu     2880

cccgagaaga ugguggcuag gcagcuaaac gauacccguu acauaucuaa auauaucucg     2940

gaaauucuga gcaagaucgu ccgcuccgac gagaaagaug agggaguaaa uucaaagaac     3000

gugauuccuu guacagguaa aaucacuacc gcccucaagc aagacugggg ccugaaugac     3060

guguggaacg aacuuauauu gccacgguuu gagagaauga aucagcugac gcagacccag     3120

uuauucacaa guuacaacga uagguuucaa aagaaucucc cgacuguucc caucgaauac     3180

ucucaggggu uccagaaaaa gcgaauugac caccggcacc augcaaugga ugcccugguc     3240

aucgcuugcg ccacccgcga gcacauuaac uauaugaaua accagcaugc acuugacaaa     3300

aacaaggaua aaaaggaaaa gcaacagagc agaaucgacc ugaggacaau acuaugugag     3360

aaaaagaaua acaauuggca guuuaaaaag ccuuggucca cuuucaccca ggagacgaaa     3420

gaagcucucg agcacauugu ggugucauuu aagcaaaacc ugcgggucau caauaaagcc     3480

acaaacuacu augaaaagug ggaggaaaag aauggaguuu uggugaaaaa gaaagugaag     3540

caggagggca ccaacugggc gauccgaaaa ccacugcaca aggagacugu aagcgguaag     3600

auugucuuag auagaaaaau agggaaggac aaaauccuca cagccacccg caaggaagug     3660

gauauuacuu ucaccgagaa aaucauuucu uccaucacag acacgggcau acagaagauc     3720

cugcuuaauu accugaacua uaagggaagu cccgaaguug cuuuuagcgc agagggcuug     3780

gaggaacuca auaaaaacau ugcccaauac aaugauggga agaaacauca gccuaucuau     3840

aaggugagga uuuucgagga aggaucuaaa uuuccacugg gugagaccgg cgcuaaggcc     3900

acuaaguucg ucgaagcagc gaaagggaca aaccuauucu uuggagugua ccagggcaag     3960

gagaaacguu ccuacgccac caucccccug aaugagguua uagaacggca gaagcaaggu     4020

cuuccgucag ugccugagag aaacgaaaaa ggggagccac uccuguucag uuugagcccc     4080

aaugaccugg uauauguccc uauggaaggc gagauugcug agacaaucga cuuuaacaac     4140

uuaucgaagg aacagaaaga gaggaucuac aagacugugu ccuucaccgg aaaucagugc     4200

uuuuucgugc aggaagccgu cgcaacgccc auuguuaaca agauggagua uucuccacuc     4260

aauaaaaugg aacgcgauau ccugggcaca augauaaagg agguguguau uaaacuuaag     4320

guagaccgac uggggaacau caccaaagcu uga                                  4353


<210>  3379
<211>  4353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3379
augaagcaca uccugggccu cgaccuuggg agcaacucca uuggaugggc cuucgugcag       60

caagauuuug agaauaaaca ggguaagaua aucgcugcag gcucuagaau uauccccaug      120

gaccagaaaa ugcugucaaa cuucgaagag gggaccacag ucagucagac ugccgaaagg      180

acgaccuacc ggagcaugcg ccgauugcgu gagagacauu uacugaggcg ggaacgccua      240

cacagaguuc uccauauucu gggauuucuu ccuccacacu augaugcgca aaucgacuuc      300

acaaagaggu acggcaaauu uauagauaau gcugagccga agaucgccua uaacaauggu      360

aacuucauuu uuaugaacuc cuucaaugag augaucgaag acuuuaagga gcaucagccc      420

cagcuguucu acaaaaaguc uaauggggaa gagucgaaaa uuccuuauga uuggacuauc      480

uacuauuugc ggaagaaagc acucucacag aagauaaccc aacaggagcu ggccuggcug      540

auucuccacu uuaaccagaa gcgcggauac uaccaguuac gaggcgaaga ggaaacagag      600

aauccaaaca aagaaguggc uuuccacagc cugaagguag uggacgucga ggcagaggcc      660

cccaauaaaa agggcgaaau cugguauacc auuagacuug agaacgggug gaucuacagg      720

cggacuagca aaaucccuuu ggaugacugg aagggaaaaa cacgugauuu uauugugacc      780

acggaccuga augaugacgg uacuauaaag cuagacaagg aaggcaaaga gaagcgcucc      840

uucagagcuc cagaugaaaa cgacuggaac cuccugaaaa agaaaaccga gcaugauauc      900

gacuucucug ggaagcccgu uggaacauau auuuacgauc accuucugca aaauccuaac      960

cagaagauca aaggcaaguu ggugaggacu auagagcgga aauauuacaa ggacgaacuc     1020

aaagccauuc uggcgaagca gaucgcauua caaccagagc uguuuaccga aacacuccuu     1080

gccgauugca uccgagagcu guaucgcaag aaugaaaccc agcagcagaa cuugcuaagc     1140

aaagacuucc ugcaucucuu ugucgaggau auuaucuucu accaaagacc gcugaggucc     1200

aagaaaucaa cgauugcuaa uuguacucuu gagaagcgga guuacauaga caaagaaaca     1260

aacgagcgca aggauacccc caucaaggug ugcagcaaau cuaauccuua uuaccaggaa     1320

uuuagaguac ugcagugguu gcagaaccuc aggauuuaug agaucgagac ugaccgugaa     1380

guuacagccg aguucaucga aaccgcacaa gacuacgagg cucuguuuaa uuucuuaaug     1440

ucccagaagg aaauugauaa cgagucacug cuaaaauauu uucucuucgc caagaaucca     1500

cagauaaaag acaagacggc gaaaaucgag cugaagaagu ggauuaccac auaccggugg     1560

aacuaugugu acgaugucgc cgacaauagc aguaaaaagu accccaugaa cgaaacuggu     1620

uaugaucuuc agcgauacau gcaaaaagug gagggguugc cuaccaauuu ucugacauuc     1680

gacacuaccu auagacucug gcaucugcuu uacucuguga aggauaaagu cgaauuugag     1740

aaggcacuga agaaauucgc uaagaaaaag caguuggaca aaacauccuu cguugauagc     1800

uuuaagaacu ucaagccaua ucccaacgaa uacggaucgu uuucugagaa agccaucaag     1860

aaacuccugc cguuaaugag guucggcaau cacugggacu uuaaccgcau ugaugugucc     1920

acccagaagc ggaucgacga ucugauaaau ggcguagcua acgaggacau cagaacuauu     1980

aucagggaaa aagccgagaa guaucagcua aauucagaaa cguguuucca agaccucccu     2040

cuguggcuug cacaguacgu ggucuacaac cgccacgccg aggcuacaga uauugaaaag     2100

uggaccagug ugagcgacuu ggaggcguau cuggaggauu uuaaacagca cucccuccga     2160

aaucccaucg uugaacaagu ggucacugag acccugcggg ugguacguga cauauggcag     2220

caguacgggg gaggucaggc aaacuucuuu gaugaaauuc auaucgaguu aggcagagag     2280

cugaagaaaa cagccgaaga gaggaagcaa cuuucugaca aaaaucagga aaacgagaau     2340

accaacuugc gcauuaagaa acuccuggcu gaacuaaagg aggaugccag cauccagaau     2400

guucggccau auucacuggu gcagcaagag cuccugaaga uauacgaaga gggggucuuc     2460

aguuccacug acgaaaucga ggaagauauu cagaaaauca gaaagaaagu ggagccuaca     2520

acgcccgaga uucagaggua uaaacuuugg uuggaacaga aauacaagag cccauauacc     2580

ggagagguca ucucucugac uaaguuauuu acagaagagu acgaaauaga gcacauuauc     2640

ccccaaucac gauacuauga cgauagucuc agcaacaaag ugaucugcga guccgcaguu     2700

aauaagcuga aaaccaacca gcuuggccug gaguucauuc agaaccauca cggugaaaag     2760

gugcgcauca cagccaauaa agaaguaacu auuuuuaccg aggagcaaua caaggaguuc     2820

gucaagaaac acuaugcuaa caauucugcc aagcagcaga aauugcuccu gaacgaaaua     2880

ccugaaaaga ugguggcacg gcagcuaaau gacacgagau acaucucgaa auauauuagc     2940

gagauccugu ccaagauugu uaggucagau gaaaaggacg agggggugaa caguaaaaau     3000

aucauaccau guaccggcaa gaucacaacu gcgcucaaac aagacugggg acugaacgau     3060

gucuggaaug agcuuauuuu gccgcguuuc gaacgcauga accagcugac ccagacacag     3120

uuauuuacuu cuuacaauga ccggcuccaa aagaaccugc ccaccgugcc uaucgaguau     3180

agccagggcu uccagaaaaa gagaauugau cauaggcacc augcuaugga cgcccuugua     3240

aucgcaugcg ccacgcgaga acacauaaau uacaugaaca aucagcacgc ucuggauaag     3300

uccaaagaca agaaagagaa gcaacaguca cggauugauc uacgcacaau ccucugugaa     3360

aagaagaaca acaauuggca guuuaaaaag ccauggagaa ccuucacuca ggaggccaag     3420

gaggcacugg aacauaucgu gguuagcuuu aaacaaaacu ugagggucau uaauaaggcu     3480

acaaacuacu augagaaaug ggaagagaag aauggggugc uggugaaaaa gaaggucaaa     3540

caggaaggaa ccaacugggc cauacggaag cccuuacacg aggagacuuu cucugguaaa     3600

aucucccuca aucgagaaaa gguuggcaaa gggaacauuc ugaccgcgac aagaaaggcc     3660

guggacagua gcuuugaugu gaagaaaauc ucuaagauua cggacaccgg aauccagaaa     3720

auacuucuga auuacuugaa guauaaaggc uccccugagg uagcuuucuc agcagaaggu     3780

cucgaggagc ugaacaagaa uaucgcccaa uacaacaaug ggaagaaaca ucagccaauu     3840

uauaaggucc gcaucuacga aaaaggcagu ggaagguuug ugcuaggcga gaaggggacu     3900

aaaagcaaga aauauguuca gggagcuccc aaccuguacu ucggugugua ccagggcaag     3960

gaaaagcgua cauaugccac cauuccgcuu aaugagguca ucgaacggca aaaacagggg     4020

cucccuucgg ugccagagag aaacgagaag ggagaacccc uguuguuuuc ccugucuccu     4080

aaugauuuag uuuacgugcc cauggagggc gaagcaacag agacuauaaa cuucaacaau     4140

cucaacaaag aacagaagga gaggauuuau aaaaccguaa guuucacggg uaaucagugc     4200

uuuuucgucc aagaggcggu ggccucacca aucgugaaca agauggaaua caguccucug     4260

aauaagaugg agcgcuccau cgucgggauu augaucaaag aaguuuguau aaaacuuaag     4320

guggaccgac ugggcaacau uacaaaggcu uga                                  4353


<210>  3380
<211>  4362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3380
augaagaccg ugcugggccu cgaccuuggg acaaacagca ucggaugggc ccugauucag       60

cacgauuucg acuccaaaaa gggugagaua uugggcaugg ggucuagaau cauucccaug      120

ucacaagaua ucuuaggaga auuuggcaaa gguaauagug ucagccagac ugcugacagg      180

acgaaguucc gguccgcacg ccgacugcgu gagagacauc uacucaggcg ggaacgccug      240

cacagaguuc uuaacauucu gggguuuuug ccuaggcauu acgccgcgga uaucgacuuc      300

gagaaacggc ucggacaguu uuucgaaggc aaggagccaa agcuggcuua ugauaauaac      360

caguuuauau ucaccaaauc uuuuggcaag augcuggccg acuuccgcca acaccagccg      420

gauuuucuca aagacgagaa gucgaaugau uuacugaucc ccuacgacug gucaauuuau      480

uaccuucgaa aagaagcauu gacaaagaag aucgagaaag aagaacuggc cuggauuauc      540

cuaaacuuca aucagaagag aggguauuac cagcucaggg gagaggaaga ggaagagaac      600

cugaauaaac uuguggaauu ucauagccug aagauaauug auguaguggc ugacgagaaa      660

uugaacaaua agggugagac uugguauagu cuccuguuag aaaacggcug gaccuaccgg      720

cguuccucua aagucccucu guucgacugg aaggagaagg ugcgcgauuu caucguuaca      780

accgacauua augaugacgg gagcgugaaa acugauaagg aaggaaacga gaaaagaucc      840

uuuagggcac caucagacga ugacuggaca cuccuuaaga aaaagaccga acaagauauc      900

gacaagaguc ggaaacccau cggcgccuac auuuaugaug cuauacugaa gaauccuaaa      960

cagaagauca acgguaaauu gguccgaacg guggagcgca aguucuacaa ggaggaacua     1020

aaacugauuc uccagaagca gaaagaguuu cacccagaac ugcaaagcac ugaccuuuau     1080

aacgagugca ucagagaacu guacaagaau aacgacgccc accaguugca gcucaauaaa     1140

aaggauuucg uacaucuguu uuuagaggac auaauuuucu aucaaaggcc ccugcggucu     1200

cagaaguccu caguugggaa cuguccucua gaguuucgca aauucaagga uagcgaagga     1260

aucgagaaag uggaauaccu caagaccauc ccaaaaagua auccguauuu ucaggaguuc     1320

agaauuuggc aguggaugua caaccugucu aucuacaaga aagacgauga cgagaauguc     1380

acaagggauu uucuuaagac uauugaagac ugggaggauu uguucgaauu ucugaaccac     1440

cguaaggaca uagagcaaga aacccuccug aaauuccuuc ugggcaagaa uggcgugaaa     1500

gggaaggcgu ugaaagugga ggcaggaaag uuccggugga acuaugucuc cgauaagauc     1560

uaccccugca augagacaaa agcccucauu aucagcaagc uggaaaaagu uaagggugug     1620

gaggaaaaau uuuuaaccga ggaaaucgag uauaagcugu ggcaucuaau uuacucggua     1680

acggacaagg uggaguauga aaaagcucuc aaggccuucg caggcaaaca gaagcuggau     1740

cuugucucuu uuguggacuc cuucaaacga uuuccuccau ucaagaacga guacggggcu     1800

uauucagaaa aggccaucaa aaagauauug ccccugcuca gaacuggaaa aaguuggaau     1860

uggauggcga uugacagcaa gguuagggau cgcaucaaca aaauuaucac aggcgaguuu     1920

gacgaagaga uaaagaauaa agugcgggag aaggccgaaa agcacucccu gaaaaaggag     1980

aacgauuucc aggguuuacc ucuguggcuu gcacaguacg ucguguacgg gagacacucu     2040

gaagcuagcu uuucaggcaa auggaacagu guagacgauu ugaagaaaua ucucgaggag     2100

uucaagcaac auucccugag gaauccaauc guugaacagg ugcuaaccga gacucugcgc     2160

gucguggccg acauuuggca guuuuacgga aagggcgaaa aagauuucuu uagcgagauc     2220

cacauugaac ucgggcgaga gaugaagaac acagcugagg accggaaaga aaugucugcc     2280

aucauacagg caaaugagac cacgaaccug cguauuaagg cccuuuugaa ugaacuguua     2340

caagauaaaa aggucgagaa cgugagaccc uauucaccga gucagcagga aauccucaag     2400

auuuacgagg acggaguucu gagcuccgau aucgagauac cugacgauau ccagaaaauu     2460

ucuaaggcug cgcaacccac caaaucggaa cuucagaggu auaagcugug guuggagcag     2520

aaauaccgca gcccauauac aggugcaaug aucccucucg gcaagcuguu cacuuccgaa     2580

uacgagauug aacauaucau accccaauca cuauacuucg acgacaguuu uucuaauaag     2640

gugauuugug agagcgccgu aaacaaacug aaggauuccc ggcucgggau ggaguucauc     2700

aaagaaugcc acggaauggu cguggagacc ggcuuuggua agucaguuac aguguucgaa     2760

gaggagacuu auagagacuu ugucgugcag aauuacagca aaaaccauuc uaagaagucc     2820

aaacugcuuu uggaagagau cccagaaaag augauugaga ggcagaugaa ugauacccga     2880

uauaucagua aauucauuag cucuguacug uccaacauag ugcgcgaaga gguuaaugac     2940

gaugggguca acucaaagaa uaucgugccc ggcaacggaa aaauuacaac ccaguuaaag     3000

caagacuggg gccucaacga uaucuggaau gagcugauuc uuccucgguu ugaaagacug     3060

aacgugcuaa cugacaguaa acacuucacg gcuuggagcg agaaucacca gaggcuccug     3120

ccaacagucc cgaucggguu gucgaaggga uuuuccaaga aacguauaga ucaucgccac     3180

caugcccugg acgcauuagu uaucgccugu gcuucucgga accacauuaa uuuccucaac     3240

aaugcccacg caaucgauaa gaaaaagaac agcgaagaga aacagaaguu uagacaugac     3300

cugaaggcga uucuuugcga caaaaaguac ucagauaaaa gugaaaagaa uuauaggugg     3360

aucuucaaaa agcccuggga caacuucacc auagauucca agaaugcucu ggacaaaauu     3420

aucgugucuu uuaagcagaa cuugcgaauc auuaauaaag ccacuaaccg guacgagaag     3480

uggguggauc gcgacgguau aaaaguaaag gaguggcaca agcaagaagg caucaauugg     3540

gcaaccagaa aaccucucca uaaggauaca gucagcggga aaguggaccu gaagaggguu     3600

accgugccag agggaaaaau ucuaacugcc acacggaagu cccuggauac gucauucgac     3660

cuuaaaguca ucgaaagcau uaccgauacu ggcauccaga agauacucaa gaacuaucug     3720

acaaguaaau cuaacaaucc cgaguuggcu uuuuccagcg aaggugugga ggacaugaac     3780

aagaauaucc gaaaauacaa cgaugggaag cugcaccagc cuauuuacaa aguuagaauc     3840

uucgaguuag gcucgaaguu uccacucgga caguucggca auaagaaauu uaaguaugug     3900

gaaaccgcca aagggacaaa ccuguucuuu gcaguauacg aggacgaaaa uaagaaccgc     3960

aauuaugaga cuauuccccu uaacgucgug aucgagaggc aaaaacaggg acugucaucu     4020

gugccgauaa agagugaaaa gggccauaaa cucuuguucu accugucccc uaaugacauu     4080

gucuauguuc cagguagcaa cgaggaauuu ucuaucgauc gucuauaccg guucaccgac     4140

agcuccgaua agacggcuaa uuuuaucccc cugucaguga guagccuuau uuucucuucc     4200

aacaaaaaug agcagaagaa aaucgggaua ucauauccua uucaagacga guucggacuc     4260

ggcagucccc agagcaagaa ccagaagucc aucgauggua uuaugaucaa agagaagugu     4320

guaaaaguga auauagacag acugggcagg gucucuuuuu ga                        4362


<210>  3381
<211>  4368
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3381
auggccaaga uccugggccu cgaccuuggg accaacagca uuggaugggc uguggucgag       60

gaucagaaua acaaaacaac ucugauagac aaggguguua gaaucuucuc cgaaggcgug      120

aaaauugaga aggggaucga aaaaucuaag gcagccgaga ggacggauuu ucggucagcg      180

cgcaagauua aauuccgacg uagauugagg aagcaagaaa ccuuaaaagu acugaucgag      240

aauaacaugu gcccccuaac aauagaggaa uggacuaagu ggaaaaagga caagaaagag      300

uacccuaaga auccagauuu uaucaacugg cucaaaaccg acgaacugaa gaauauuaac      360

ccguauuucu uucgggauaa agcuagucgc acagagcuuc cccugaugca gaccggcaga      420

gccuuguacc accucgcaca gaggcgggga uuccugagca aucgccugga ccaggguucc      480

gauggguuua cugagcaaau ccgacucgac uuaauuaaga aaaucgaaug ugagacagau      540

aagaccgaac ugcuuacguc ugugcagucg auaauugaca cuuauucaga ggaagaggau      600

aaggacgcca aaaaguugcu gaaaggaguc gagaagcuau gcaaaaucaa caaugaacuc      660

ccuauuaccg aguucaagga aaagcugauc aacuaccuua auaaagcuga caaccugggc      720

gcagugaaga aaggcaucgc cgauguuagc aagaaaauua cagagacugg gugugaaacc      780

uugggacagu auuuuuacaa gcucuaucag aaucaaagua agauaagaaa aguguacaca      840

gcuaggaccg agcauuacua ucacgaguuc cugaagaucu gcgaaacgca gaacauuugu      900

gaccaguuaa aacauaagcu ggauaaagcc aucuuuuacc agcggccacu caagucccaa      960

aagggucuua uaggcaaaug cgucuucgag aagaacaaaa cucguugugc ggugucucac     1020

cccgcauaug aagaguaccg cgcccugcag uuuauuaaua gcaucaaggu auccgaaaca     1080

aaagacggga agaaagauuu cuugucauuc gacgagagaa agaagaucug gccuaaauuu     1140

auuaggaaga guaaaccaaa cuucgauuuu aaggagaucg cugaaacccu agacaaaaag     1200

ggacugagcc gguauuacaa uuauacuggc cauacaucug uuuccgguug ccccaccauu     1260

gcccagcuca cgaacauauu cggggagaau uggcaagaaa cccuguuuga gaaguacaca     1320

aacaaacaga auacuaaggg acuuaaaacc aucgaugagg uggucacaga cauuuggcac     1380

gugcuguuca cuuuugauaa ggacgaaaag aucaucgagu ucggcaaaca gaaguugggc     1440

cucaacgaag ugcuggcuaa aaaguuuggg gcaauuaauc ugaaaaagga cuacgccuca     1500

cugagccuaa aagcgauaaa caagauccuc ccuuaucugc gagagggacu uauuuacagu     1560

uaugccgucu ucuuggcaaa uaucgaaaac guugugggug aggagauuug gaagaaugau     1620

gaaaacaaaa aucugaucaa gaaagagcuc cacuuacuua uagacaacuu uaacgaaauc     1680

aagggccugg aggcuguagu gaaugauuuc auugccgacu uuaaaaagga auacaacaau     1740

gcucauaaag auuaugucuu ggacgagcag gauaagaaga uccuccugga gaaaauugaa     1800

ucuaacuacg ggaagaauaa auucgagucc uuagaaccag acaagaaaac cgagaucaua     1860

aacagcacag aaaagauggu ggccgagcaa cugcgcaagc agagaggaga guucauuaaa     1920

ccgaagaggc uagaugagaa aaucucggaa uuucucauua auaacuucaa uguuaccaag     1980

aaaucuaucg cacggcugua ucaccccucc gacguggaaa uauuuaagac ugccucacgc     2040

aacagugagg gccuuuugcu gcucgguagc ccuaucgucu ccucuauuaa gaauccaaug     2100

gcuaugagag cgaugcauca gcugaggaaa uuagugaaca cgcugaucaa ggagaauauu     2160

aucgaugaag agacaaaaau aaacauugaa cuugcacgug aguugaauga cgccaacaag     2220

cgggcugcca ucaaaaagug gcaggaugaa cgacaaaagg caagagagga guacaaaaag     2280

agcaucaaag aacucuucaa ggccgagugu gggaaagaca uugaacccuc agacagugag     2340

auccugaagu acgaacuaug ggaggagcag cagcacauuu gcauauauac cggcaagacu     2400

aucaacauuu gugauuuuau cggagcuaau ccuaacuucg acauugaaca caccauccca     2460

aaaucccugu gcuuugauaa uagcaacgag aaucucacac ugugcgacau acaguacaac     2520

agggauguaa agcgcaauaa aauccccacc gaacuuucug acuauaacuc aauuaagccg     2580

cgguuggaua aauggcuggu uuucaucgac gauuacgaga aguuauauaa uacuaggaaa     2640

aaggccaagg gcauugaaac aaaagagcaa aaggacaaaa gaaucaagga gaaacauuac     2700

uauaagcucc accuggauua cuggaagggg aaauacaagc gcuuuauaau gguggacguc     2760

ccuaguggau ucaaaaacag ccagauugug gauacgggua ucaucaccaa guaugcaauu     2820

uccuacauga aaucuauauu ucccaagguc uauucgguga agggcaauau cguugcggag     2880

uuccgaaaaa uuugggggcu ucagaacgac uacgaaaaga aagaacgggu gaaucaugua     2940

caccauugua ucgacgcuau uacuaucgcc ugcaugagca agcagaaaua ugauacacug     3000

gcaaagauau accacgagga cgaggccgga uuucacaaga ucaaaaagga aacccaauug     3060

gucuccaaac cauggacauc auucacuaug gaugugcuca acauugagaa ggaaaucuuc     3120

guuagucauc acaccccuga caauaugaaa aagcagucua aaaagguggu ccgugagaga     3180

ggcauuaucg cucccaaggu gauauaugaa acggaugcca acaacaauac ccugagggac     3240

aaauacggua agaaaauugu aaaggaguac auccuacuga aagaugugaa cgggaaucuc     3300

aucccaauua agggcaagcg ccugacaccc agcgacauca uaaacaaagu ugauggaauu     3360

gacuauuuuu cccuuaucau uaaugauaag auccaguacu augaguucgc aaaaucaaag     3420

aaaacuggca agauaauuua caagaaagaa ccuaucuauu ugcaagggga caccguccgg     3480

ggaagccugc auuuagacac auuuuacggu gcuaucgcca aggauauuaa cggcaauauc     3540

auuacuaaca ccaaugggaa aauagcgcca aacuauguga ucagaaagga gcucucuaaa     3600

cugaaggaaa cggacauuga gaauaucgug gauccggucg uuaggggaaa gaucauugaa     3660

gccaaaaaga acaaacuuau aacauucaau aaguccggcg cugugaucaa aaccacuauu     3720

uggaugaagg agaaccagau ccccauuaau aaagugcgag uauacaguag ccugaagaac     3780

ccucuaccag acuuuaagaa acaccgcgau cucucucacc ugacauacaa gcagcaguau     3840

aaugucucca acgaggaaaa cuacuguuug gcaaucuaug aggguaaaaa uaagaaaggg     3900

aacgugugcc ggaccuguga acugguuaau augauagacg ccggcaacua cuauagauua     3960

ucaaauaaga acuggaagca acaguacccc aucgugccug agaaagauag ugucacuaau     4020

uauagccuca agaccauuau cacaaaagga cugauggugc uucuguacga aaagucgcca     4080

gaggagauuu gggaauugac guacaaccag cagauggaga ggcucuauga aaucacccaa     4140

cuggacacug agaaauccgc ugagauaaag cuacuguucc aucaggaagc ccgugagaag     4200

aaaguuauua cagaauacau gggccuuaag accgggauga aaggaggcaa gaauaucggu     4260

aaacacaagg aguuucccug gauuaaggug gggccgaacu cuuucgaugc acucaucgaa     4320

ggaauagacu uuaaacugac accuacuggc aagaucauua aauuguga                  4368


<210>  3382
<211>  4374
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3382
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc caaagugucc       60

gucgaugacu acgguaauua ucugcacgau auaaaguugg gcucuagaau cauucccaug      120

ucacaggacg uuuuagggaa cuucgauagu ggagugacug uaagccaaac ggcucagagg      180

accgaguuuc gguccaaacg ccgacuggug cagcguaauc uacagagaag ggaacggcuc      240

caucgcgucc ugcacgugcu ugacuuccug ccugcacauu acgaugccgc gaucggcugg      300

gacagaacag aggcuguuac uuaugguaag uuuuugaacc acgccgaacc aaaacucgca      360

ugggaggaac uggggaaugg aaagaggcgg uuccuguuua uggauucuuu ccaugagaug      420

guggccgacu uugcucgcug ccaaccggca cucgucgccg auggcaagaa aauucccuua      480

gacuggacca ucuacuaucu gcgaaagaaa gcucuuacac aggccauaac caaggaggaa      540

uuggcgugga uccugcuaaa cuucaaucag aaaagaggcu acuaucagcu caggggagag      600

gaagaggagg aaaacccuac uaagcaagag gaauacuacg agcugaaggu gguaucgguu      660

gaagcaacag auucacggaa agggaaugac accugguaua acgugcaccu ugagaauggu      720

uggauuuacc gucgcagcag uaagaucucc cuggacgauu gggucggcaa agugaagcag      780

uuuauuguga cgacugagua ugaaaaagac ggggccaccu ugaagacaga uaaagaggga      840

uacgucaaga gaucuuucag ggcuccaaac gaaaaugacu ggggccuccg gaagaaacga      900

acugagaccc uguuagaaau gagcgguaag acaguugggg ccuuuaucua ugaucaucug      960

cuccaggagc ccgcagacaa aauacgcgga aacuucauua gaaccaucga gaggcacuac     1020

uauaaggcug aacuugaggc cauucuggcg gaacagugua aauaccacau cgaguugcgg     1080

gauaaggaaa ugcuagaggc cugcgcacgc gagcuguacc ucaauaaccu gccucaucaa     1140

agaucccuua ugaagaaaga caugacguau cuguugcucu acgaucugau cuuuuaucag     1200

aggccauuaa agaguaaaaa ggcucugauu gccaacuguc ccuacgaaua uuacgaguau     1260

guggacaaag aaacuggcga gauaaagcgu cagaauauca agugcauugc uaaaucaaac     1320

ccuuacuucc aagaguuccg gcuauggcag uuuguacuca aucugcgacu uuucgauaga     1380

guggaagaca aggaggucac agccaacuac uugccaaccg cagacgauca cgccaggcug     1440

uuuacuuauc ucaaugaccg caaagaaauc aaccaggaga cacugcuuaa ggauuacuuc     1500

aaacugaaga aggugaaaau aggcaaugaa aaggaguauc cgauucggug gaacuacauc     1560

gaggacaaag aaaagaaaua ucccuguaau gagaccagau acgaauugcu cauggcucug     1620

gauagggcgg ggaucgagca caagugguua gacgauaaca aucuggaaua ucgccuaugg     1680

caucuccugu acagcguuga ggugaaaucu gaguccgaaa cggcacuucg aaaguugaag     1740

aacgacgaug ccuuugucaa uucauuccug aaagugaagc cuuuuaaaaa ggaguacgga     1800

gcuuauagcg aaaaagccau uaagcggcuc cuggcaguaa ugcguaaggg uuucuuaugg     1860

aacgagcagg acaucugcga ugaaaccaga aaaaggauug agauaaucau uaagggcgac     1920

guugaugaga aagugaagga acgcaugggg cacgccgcuu acaguuuuga ccgggucucu     1980

gacuuccaag gacugccaga guggcuugcc uguuaugugg ucuacggcag acauuccgaa     2040

gcaauggaua uccagcacug ggacagcccc gagagguuga uggcguauau caauaacuuu     2100

aaacaguacu cgcuccgaaa cccuauugug gagcagugca uacuggaaac acuacgcacu     2160

guucaugaua ucuggaagga gaccggucaa auugacgaaa uccacgugga gcuggggcgg     2220

gaaaugaagu cuacauccga ggagagagcu aggcguacuc agaauguacu ccgcaacgaa     2280

aauaccaacc ugcggauuaa aucacuuuug aucgagcuga agaaugauac agacauaugu     2340

aacguccacc cauauagucc caugcagcag gaaaucuuaa gaauuuacga ggaaggcgcc     2400

cuccaagagc ugaauaggga ggaugaagac uucgaggaaa ucagcaaaau uucccagaug     2460

ucucagccga gcggaaagga gcuuaaccga uacaaacugu gguuggaaca gaaguaucgg     2520

ucaccuuaca ccggcaaggc aaucagucuc gccaaacugu uuacucccgc uuaucaaaua     2580

gagcauguga uuccacaguc ccgcuacuuc gaugacagcu ucacgaauaa gguuaucugc     2640

gaggccgaag ugaacacacu aaaaucuaau cugcucgggu augaguuuau uaagaaacac     2700

ggaggugaaa ucauacauug uaccacucug ggcgauguca gaaucuucuc agagagugag     2760

uacaaggcau uugugaugga ccacuaugcu aacaauaaaa gcaagaagga uaaacuuuug     2820

auggaagaca uuccucagga guuccugucc aggcaaauga acgacucucg guacaucucg     2880

aagguaguga aaaccuuacu cagcaauauu guucgaaugg ccgaugaauc cgaggcgaca     2940

ucaaagagug ucaucgugug caccggggga gugacugaca gacugaaaaa ggauuggggc     3000

cuuaacgacg ucuggaacuc uauaguuuac agccgcuuug aaaggcugaa ucgucuaaca     3060

gguacggagg ccuucgggca cugggagaac aaggauggca aacggguguu ucagaccucc     3120

augccccucg aacugcagag aggauucagg aagaaacgca uugaccaucg acaccaugcu     3180

auggaugcau uggugaucgc cugcgcuuca agaaauaucg uaaacuaucu gaauaacgag     3240

agcgccaaua acccaaagaa acgggaagac uuaaggcgcc agcucuguga uaagaaucgu     3300

gucauuagaa agcccuggga gacuuuuaca caagacgcau acaacgcgcu ggaaaaugug     3360

guugugucuu ucaaaaacua uguccgggug aucaauaagg ccaccaacag guacgagcac     3420

uaugaugaga auggcaaaaa gcgcuuuguu cagcagaaag gguccgaacu uugggcuauu     3480

cgaaagccua ugcauaagga gacauacuau ggacacguga accugagacg gaaagaauug     3540

guaacucucc agaaggcccu ggacgacaua ccaaguaucu gcgauaaaaa gcuaagggac     3600

uacauuagcg cacuggucga gaaacacuuc aacaagcgcc aacuucucgc ucauuuuaag     3660

ucuaccgauu acuauuggca gaaacaguac gugaauaagg uguaugucug gacguucucc     3720

gacgauauca aaccgcuggu ugccaccaga aaguugcugg acacaucauu cgaugcaaaa     3780

aggauuggua cuaucaccga cacaggcaua cagaagaucu uacggaacua ccucgaagcc     3840

aaagggggaa augcuacucu ggcguuuacc cccgagggca uugccgagau gaaccaaaau     3900

aucaguaagu auaacgaugg uaagccucac cagccaauuc uuaaagugcg uaucacggaa     3960

cccauggggg caaaguaccg cguaggccag acaggaaaua aaauggcuaa guauguggag     4020

gcccaaagcg gcaccaaccu guacuucgcc auauacgaag acucggaggg gaaacgaucc     4080

uauacuacag ucccuuugga ugagguggcu gaaagacuca agcagaggcu gucuccaguu     4140

cccgagaagg acgaaaauga ugugccgcua aaauuuuacc ugagcccuaa cgaccuuguc     4200

uaugugccaa ccgaggagga acuccagcug cgggagugua aguugaauaa agaccgcauu     4260

uacaagaugg uuucaaguac uggaaacgaa ugccauuuca uccccuauua cguggcaaau     4320

gucaucuaua acaaaggcgg uuuuaauaug cagaugcaca gaccucaauc cuga           4374


<210>  3383
<211>  4374
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3383
auggccaaga uccugggccu cgaccuuggg accaacagca uuggaugggc uauaguggag       60

aaaaauaacu ccgauuucac acugaucgac aaggguguca gaauauuuuc ugaaggcguu      120

aaaacugaga aggggaucga aaggucacgg gcagccgagc gcacgggaua ccgaagugcg      180

cguaaaauua aguauagaag gaaguugcgg aaauacgaaa ccuuaaaggu gcugagcaaa      240

aaucgcaugu gcccccuaca gaucgaggau guagaggcuu ggaagaaauc cggcuucaag      300

gacuauccuc ucauaccaga auuucugcaa uggcuuagaa caaacgauca cgagaaugug      360

aacccguacu ucuuuaggga caaggccucu cgggcaaaag ucucgcugug ggaauugggu      420

cgcgccuucu aucauaucgc ucagcgaaga ggguuucucu caaauaggcu ggaucagagc      480

agugagggag ugcuggagga acacaacccc cagauucaau cccucaucga ggacuuagaa      540

gagacuucua ccauucugaa ugaaguucgg gauuacuaua ucaaccuugg cuugcuggac      600

ggcgugaagg gggauuucaa aaaggagcua gacgagggag agaaaaagcu caaaagccug      660

uacaauacac uuauagcaau uaucaagaag aacgaaaaug auguccagaa auguaaggac      720

gaaauuauca aacgucugaa caagaaagag gacuugggua aggugaaggg caaaaucaag      780

gauauuuccc aggccaugcu cgacgggaau uuuaaaaccc ugggacagua cuucuauuca      840

uuauacaaca agggcaaaau acgcaaccaa uauaccagua gagaggaaca uuaccugcag      900

gaguuuauca caauuugcaa gguacagggu aucaauggga uaaacgauga agagauucuc      960

ccugaaaaga aauucaccgg acuugcuaag gaccuguaua aagccaucuu uuuccagagg     1020

ccauuaaaga gccaaaaagg ccuaaucggc aaguguucuu ucgagaagaa uaaaucccgg     1080

ugcgcgauuu cacaccccga uuuugaagag uaccgaaugu ggaacuaucu gaauacgauc     1140

aaguucggga cucagagcga caaaaagcuc cgcuuucuga cccaggagga aaaacuuaag     1200

cugauuccua aauucuacag aaagaacgau uuuaauuucg agguuuuggc aaaggaacuc     1260

auagacaaag gagccacauu ugguuucuac aagaguucua ggaaaaacga guuucuguau     1320

ugguucaauu acaagccaac ugauaccgug uccgcuuguc aauuuagcgc cucguugaaa     1380

aacgcaaucg gcgaggacug gcagacaaag gucuucaccu auaagacgcu gaacucugag     1440

aaaaaggaag ugauucggac uguggauuac cgcgaccuau ggcaucuccu guccgcuuca     1500

acaagugaca ucuaucuuua cgaauaugcc aaagagaagu uggggcugga agauaaaagc     1560

gcgaagaccu uuuccaagau caaacucaag aaagacuucg ccucucugag ccuuucagca     1620

auuaauaaga uacugcccua caucaaagag ggauugcucu acagucacgc ugucuucaug     1680

gccaauauug agaagguugu ggaugaacac aucuggaagg acguaaaaga gagaacuauu     1740

auccagaacg aaauauccaa gaucauuaaa agcgaugugu uugagaggga agucaucaau     1800

auuaucaacg gccugauaaa gaacucuaaa acagagggug agaauuaugc ugaagaguca     1860

aagaacauuu ucaaaaccga auuugacaau gccaucgcaa agauuuucga gaagaacaau     1920

gauaucgaau uuaaccagcc uuucuuagac gauacguacc ugaaauaugu ggaggagcua     1980

aagaaauuug acaaggcccg uaguuuccuc ccaguuccgc ggcuggauga aaaagugaua     2040

caauuucuuu uggggaauaa caaggacggc auggucuacu gcagcgauaa gaauaaaacc     2100

aagaaacugu aucaucccuc cgacaucgag auuuucaaga aaaagaucau uaaggacgaa     2160

uuuggaaacg agaaaauggu gcucggcucu ccucugacaa cuucgaucaa gaauccaaug     2220

gcuaugcgag cguuacacca gcugagaaaa guacuuaaca ccuugauacu cgaggaacag     2280

auugaugaga auacaaggau ccauaucgaa auggcacgcg agcugaacga cgccaauaag     2340

cggaaaggga uucaggauua ccaaaacgaa aacaagaagu ucagagagga cgcuaucaaa     2400

gagauuaaga aacuauauuu ugaagagugu aagaaagaag uugagcccac ugaagaggau     2460

gugcugaggu accagcucug gauagagcag gacaagaagg aaaucuacga gcagggaaaa     2520

agcauucgca ucuccgauau uaucgguuca aauccugccu augacauaga acacaccguc     2580

ccacgaagua ucucucaaaa caauagccug augaacaaga cacuuugcac uccccgguuc     2640

aaucgugaga uuaaaaagca gaaaaugccg aucgaauugu ccaacuacga ggagauucug     2700

ccuagaaucg aacacuggaa gaaagaggca gaagauuuaa cuaggcagau agagucaauu     2760

agcaagucua ucaaguccgc caguacgaaa gaggcuaagg acaaaaauau ccgccggaga     2820

cauuaucuca cacugaagag ggauuaccuu aaaggcaagu augaccgauu cauuugggag     2880

gaacccaagg ugggguuuaa aaacagccag auaccagaua ccggaaucau uacuaaguac     2940

gcccaagcau aucugaaauc uuacuucaag cgcgucgaau ccgugaaagg cgguaugguu     3000

gcggaguuuc ggaagcagug ggggauccag gaaucauaca uugacgagaa uggcuucaag     3060

cacuauaaag agaaggacag aaguaaacau acccaccaua caaucgaugc uauaaccauc     3120

gccuguauga cuaaggacaa auacgaugug uuggcacaag ccuggacaag cgaagacgag     3180

caggauaagc agaaggcuaa aacgcucaug ucggaagcca agccuuggaa aaccuuuaag     3240

gaggacauug agaaaaucga agaggaaauu cugguauccc acuucacucu agauaacguc     3300

aagaaacagu cuaagaagau cgugaggguu cguggcaaaa agcaauaugu ggcagagaua     3360

gaacgcgacg ucaauggaaa agcuauggcc aagaaagaug cgaacgggaa gauuaucuac     3420

aagcugaaug agaaaggaga aaagaucccc cggcuccagc agggugacac aauuagaggc     3480

agccugcacc aggauucaau cuauggggcc auuaaaaacc cacuuaauca agaggagauu     3540

aaguacguga ucaggaaaga cuuggaaagu cugaaggcua acgacguaga uaacauugug     3600

gacgagaccg uuaagcagaa agucaaggaa gcaaucgaga auaaaauauu acuccugucc     3660

ucuaacgccc agcagaagaa uaaacuugug gaugacgugu ggcugaacaa ggagaagcaa     3720

guccccauua aaaagguucg aaucuaugcu aauagcguga aaaacccucu agaaaucaag     3780

gagcaguccc uccugucaaa aagccggaag gaacauaaac agaaggugua cggacaaaau     3840

gaugagaacu acgccauggc aauuuaugaa uuggacggca agcgcgaguu ugagcugauc     3900

aauaacuuca auuuagcgaa acucauuaag cagaaagaag guuuuuaccc acugaacaag     3960

gagaaagaag uaaaggggaa gaaaguccuu uauccgauag ccaagaguaa uuacagagau     4020

gugguucuga aaaggggcca acaggugguc uucuaugaca aggaggugga aaaccccaaa     4080

gauaucucug agguuauuga cuuuaaggga cggaucuaca ucauugaggg cuuguccaua     4140

cagcgaaucg ugagaccuag cgggaagauu gaugaauaug gaguaaucau gcucaaauac     4200

uucaaggagg cucgcaaagc cgacgaaauu aagaaagaua auuacaagcc agacggcguc     4260

uucaagcugg gugagaacag gcccacacgu aaaaugaauc acucgcaguu uacugcauuc     4320

guggaaggga ucgacuuuaa ggugcuaccu accggaaaau ucgauaagau auga           4374


<210>  3384
<211>  4377
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3384
augaccaaga caauccuggg ccucgaccuu gggacuaaca gcauuggaug ggcccugaua       60

aaucaggauu ucgacaacaa aaagggugag aucuugggca ugggguccag aauuaucccc      120

augacgcaag auauuaaaga cgaauuugga aagggcaauu cuaucucaca gaccgcugag      180

aggacacggu uacgcggugu gcgacgucug auacacagaa cucuacucag gcgggaacgc      240

cugcauagag uccuuaacau cauuggguuc cugccugagc acuacgcaaa ucagaucgau      300

uuugaaaaga gguucggaaa guuuaagcca gagagugagc cgaaaauuag cuucgacggc      360

aacgaugugu uuuuguucga aaaguccuau caggagaugc ucguugacuu uaaaauccau      420

caaccccagc ugguaucuaa uggcaagaaa auaccucacg auuggaccau uuacuaucug      480

cggaagaagg cccucucgaa aaagaucgaa aaagaggagu uagcguggau ucugcuuaac      540

uucaaucaga agcgcgggua cuaucaguug cgaggagaag aggaagagga aacaccaaac      600

aaacuggugg aguuucauuc acuaaagauc gucgagguga augcugacga accccaaaaa      660

gguaagccug agaucuggua cagccucguu cuggaaaacg gcugggugua uagaagggcc      720

aguaagaccc cacuuuucga uuggaaagac aagauucggg aguuuauagu cacuacagaa      780

aucaauaacg augggaccgu gaaaacggac aaggagggaa cugagaaacg uuccuuccgc      840

gcacccaagc cugaagacug gacccugcag aagaaaaaga cagaguucga auugucuaaa      900

agcggcaaug agguaggugc cuuuauuuac gaauccaucc uccagaagcc aaaccagaaa      960

auaagaggga agcugauuuc aacuaucgag aggaaguucu acaaagagga auuaaagacc     1020

auccugaaaa cacaacucuu uuuccacaag gagcuuaaag augaaaagcu guauaacgcu     1080

ugcauugagg aguuguacaa gaauaacgaa gcacaccgga gucuacugag caauaaagga     1140

uuugagcauc ucuucaucaa cgacauucug uuuuaucagc gaccgcuucg cucuaagaaa     1200

ucccagauau caaauugucc ccuggaaaag agaaccuaca aaaaggaggg caucgaaauu     1260

acggagggca ucaaaguuau cagcaagagu aacccuauuu aucaagaguu cagguugugg     1320

caguggauau cuaaucucuc ccuguacugc aucgaaccaa cugagacaaa cgugaccagc     1380

acauuuuuaa auucgauuga agauuaugag aaccuguucg aauuucuaaa uaaccggaag     1440

gagaucgagc agaaacaccu ccugaaguac cuuuuggaaa aucagggguu caaaggaaag     1500

cugcucacua acgagcugga aaaauuucgc uggaauuucg ucgccgacaa gaaguacccc     1560

uguaacgaga ccgguucucu ucugcauacg agauugucca aagugaagaa cauuucaccu     1620

gauuuccuca ccaaagaaau cgagcaccaa cuguggcaca uaaucuauag ugugacagac     1680

aagauugagu acgaacaggc uuuaaaaacu uuugccagga agaauaaccu ggaugucgac     1740

agcuucuuug agcauuucaa gaaaauccca cccuuugaau ccaccuaugg cgcguacucu     1800

gagaaggcaa uuaaaaagcu acucccgcug auccgucuug ggaaauauug gaauugggag     1860

gccauagaua gcauuucaaa ggaccggauc aguaagauuu uguccggaga auacgaugag     1920

aacaucaaaa aucgaguuag agaaaaggcu gugcaccuga caagcgagaa caauuuccag     1980

ggccuccagg aauggcuggc caaauauaua guauacgaca ggcauucuga ggguaacgac     2040

uuagggaagu ggacuucagu gagugaucug gagaccuacc uuaaagaauu uaagcaacac     2100

agcuugcgca auccuaucgu cgaacaggug auuacagaaa cccuccgggu ugugaaagac     2160

aucuggauua agcauggcaa gggaacugag aacuucuuug augaaaucca cgucgagcug     2220

ggcagagaga ugaaaaauaa cuccgaagac aggaagcgcc uaacgaauac aauaaccgag     2280

aacgaaaaua cuaaccugcg aauuaaagca cuccugaugg agaugaugaa cgauaaugac     2340

guggaaaacg uacggcccua uucuccaucg cagcaggaga uccuuaagau cuacgaggau     2400

ggggcuuuga auagcaacau ugaacuggac gaugagaucg uuaaaauuuc caagaaggcc     2460

gaaccuacca aaucagaguu acaacguuau aagcucuggc uggagcagaa auacagaagu     2520

cccuauacag gacaggugau accacuuaau aagcuguuca ccucugaaua cgagaucgaa     2580

cacgucgugc cccagagcag guuuuucgac gauuccuuuu caaacaaagu cauuugcgag     2640

agcgcgguga auaagcgcaa ggacaaccaa uugggucucc aguucaucaa aaaucauucu     2700

ggcgaaaagg uugagcuggg guucggaaaa gugguacagg ucuuuacuga ggaacaauau     2760

cuagauuucg ugaaggagca cuacuccaaa aaccggagua agcauaauaa gcugcuccug     2820

gaagagauuc cugagaaaau gaucgagaga cagcuuaacg acacaaggua cauaagcaag     2880

uuuguuucuu ccaucuuguc aaauauugug cgaagugaga aagacgauga cggccugaac     2940

agcaagaaua ucuuaccagg uaacgggaaa auuacgaccg aacucaagcg cgauuggggc     3000

cugaacgacg ucuggaauga ucuuauccug ccgcgguucg agagaaugaa cacuauaaca     3060

aauucggacc uauuuaccac auauaacgau aaguaccaga aacaccuccc cacugugccu     3120

uucgaauauu ccaagggauu ucagaaaaag aggauugacc accgucauca cgccauggau     3180

gcacugguaa ucgcuugugc cacccgcgac cauuugaauc ugaugaacaa ucaaucugcu     3240

aaaagcgagu uaaagcggua cgaucuccag aacaaacuga gaaagaagga accauauuuc     3300

aaucagaaag agaacaagca gaaagaggcc uuuaaggacu ucaucaaacc cuggggcacg     3360

uuuaccgaag auucaaagaa ugcacuugag aagauuauaa ucaguuucaa acaaaaccug     3420

agggugauua auaaggccac aaacuccuac gaaucuuaua aagacgagga cgggaauuug     3480

cgaaucggaa aggaugguaa accugagaag ggccucauua agcagaaagg gcugaacugg     3540

gcuauccgga agccacuaca caaagacacu guuagcggac agauaaaucu gucccgcauc     3600

aaacuuccca acggcaaaau ucucaccgcg acaagaaaga accuggauac uucauucgac     3660

uugaagacca ucgaaaauag cauuacggau acagguaucc agaaaauacu gaagaacuac     3720

uuacucagua aagagucucc ggaacuggca uuuuccccug aggggcuuga agagaugaau     3780

aacgagauug aaaaguacaa uaacggcaaa uuccaucacc caaucaauaa ggccaggauc     3840

uaugagcugg gaagcaaauu uaacgucggc caaaccggga aucggcgaga caaguucgug     3900

gaagcugcca agggaacuaa cuuguuuuuc gcaauuuacc aggaugagaa uaaaaacaga     3960

ucguaugaaa caaucccccu caaugaggug auagagcacc agaaguggcg cgccucacug     4020

ccuaaagagg agcaagaaaa gauuccccua gucccaguua acaaacugaa gggcaccuuu     4080

aucuucucuc uuaguccuaa ugaccucgug uacgugcccu ccaacgauga gcuggaaagg     4140

agcgcuucua uugacuuuag caaguugaaa aaggagcaga ucaaccguuu auauaaaaug     4200

guauccucaa gugguagcca gugcuucuuu gucaagucug agguggccac uuccguugug     4260

aauaaaaugg aauacucaag ucugaacaag auggaaaaga gcauagauaa ucucaugguc     4320

aaagaaauuu guaucaagcu gaaaaucgac cggcuuggga acauuuccaa ggcauga        4377


<210>  3385
<211>  4377
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3385
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugauagag       60

caggauuucg aauccaaggc ugguaaaauc ucuggcgcag ggucaagaau uauccccaug      120

agucaagaca uuuugggaac auuugagacu ggccagagcu acucccagac ggccgauagg      180

acccacuauc ggucuauccg ccgauuauac cagcguacaa agcugagaag ggaacggcua      240

caucgcucgc ucaaugugcu ggguuuccuu ccugagcacu auaaaaacga aauagacuuu      300

gagcaaagaa aggggcaguu cuacccaaau aaggagccga aaaucgcgua uuaccccgaa      360

caggucgcuu caaagguuaa auaucaguuu cuguucaaga gcagcuuuuu ggagauggug      420

gaagauuuca aaaggcgggg auacaacggc gccauuccuu acgacuggac uaucuauuac      480

cuccgcaaga aggcacugga gaaaaagauu gagaaagagg aacuggccug gcucuuacug      540

aauuuuaacc aaaagcgagg cuauuaccag cuuagagggg aagaggaaga uaccaauucc      600

aacaaaaagg aggaguucua ugaauugaaa guaucuagcg uggagcagac acaugacacc      660

uccccaucag gaacuuggua caauguccau cugcagaacg guugggugua uaggcggaag      720

aguaaggaua gccuaaaaga cugggaaggc cucaauaaga aauuuaucgu uacaacccac      780

cugaacgcug augggucuga gaaacuugac aaagauggaa augugucccg uucauuccgc      840

gcacccaagg aagacgacug gggccugguc aagaaaagaa cggagagcgu guuggagaag      900

aguggucucc acguagggga auuuauauac gagucucugu uagaaaaccc ugagcaaaag      960

auuaggggau cccugaucaa gacuauugaa cggaaauacu auaagaagga gcucgaggcc     1020

auccuugaua aacagaccag cuuccaccga gaacugcgca augacgcuuu guacaaggag     1080

gccacacuag aacuguaucc caaaaacucg gcgcauagau cuaacaucaa gcagaggggc     1140

uucaaauacc ucuuuauuga ggauauaauc uucuaucaac ggccacugaa gucccagaag     1200

caccuuauug cacauugcca guacgagaaa cgcacuuaua agaauaacgg cggggaaacc     1260

aucgagguuu cacugcaggg agccccuaaa agucacccaa guuuucaaga guucagaaua     1320

uggcaguuua uuuccaauuu gagguucuac gacaaggaag aaaucgugga gggucaggag     1380

gucucuauca cagaaaaacu cuuaaagagc gauucagacu uugcugagcu guaccacugg     1440

cugagucauc agcgugaagu ggaucaaaac aaaacccuaa aguauuucac gcagaagaaa     1500

cucauuccga agcagaaaaa ggacaauccc acuauccggu ggaacuuucc ugaggauaaa     1560

aaguacgaaa ugggcgaguu caaggcccag cugcuuuccc gauuggagaa agugagaaau     1620

ugucaaccag acaaguuucu gacacagaaa aacgaacucg aucuguggca cauuauauau     1680

agcgucaccg acaagacuga guucgaaaaa gcacuuguua cauuugcuga gaagagggaa     1740

cuggacucug agucauucgu ggagaauuuc aagaaauuuc ccccuuucaa gaacgauuac     1800

gccaguuaua gcuugaaagc gaucaagaaa auucucccac ugaugcgccg ggggaaguac     1860

uggaaggccg aaaauuuagg accccagacc cugcagagaa ucgagaaaau cguaaacggc     1920

gaagcagacg auaagauuuc cauaagggcu cgcaaacuag cccuccaucu gaaugacacg     1980

aagucuuuuc aaggucuucc gaccucguug gcuugcuaug uggccuacaa ccgacacagc     2040

gagaaugagg aaaaccagaa auggcagaca ccuuccgaua ucgacaagua ccugaaagau     2100

uucaagcaac auucacuccg gaaccccauu gucgagcagc ugaucacuga agcauuacgu     2160

gugguuagag acauuuggaa gcacuauggg cagggccaga aagacuuuuu cgaugagauc     2220

cacguggaac ugggaaggga gaugaagaau ccagccauaa aacgcaagaa acuuagucaa     2280

aagauccagg agaacgaaaa uaccaacuac cggauuaagg aggucuugag aaaucucaaa     2340

gaaaacaaua ucgaugcuaa gccuuauucu cccagccauc aggagauucu gaaaaucuac     2400

gaagagggcg uguaucaguc cauagagaac guagaagacg agauugaaaa gaucaggaaa     2460

aauaacacac caucaagcaa ggauauuaau cgauaccgcc uauggcugga gcaaggguau     2520

aucucucccu acacuggaga aguuauaccu cucucccagc uguuuaguaa ccaguacgag     2580

aucgagcaca uuaucccacg gagcagauuc uuugacgacu cucuugcgaa uaaggugauu     2640

ugugaauccu cagucaacgc auugaaagau aauaagaccg cccuggaguu caucacaaac     2700

caugguaacg aaaaagugga guuugcuggc aagaccgucg agauauuaag uagcgacaaa     2760

uauacugaac acugcaagag guacuucaag cguaaucgca cgaaacucaa gaaccugcuu     2820

ucguaugaug ugccggaggg guuuauuaau cggcagcuga acgacuccag auucaucucu     2880

aaauacguua ggaauuugcu cagcaacauc gugcgagaag aggaggaacg ggaggccauu     2940

ucaaagcaca uccugacaau ucccggaagu auaaccuccc aacuaaaaca ggauuggggc     3000

cugaaugaaa aguggaacga gguagucacu caucgcuucg aaagacucaa uaaccugacc     3060

gguacaaccg acuuuggguu cugggauaau aagaucaacg cauuuaggau uacugugccu     3120

aaagagcuuu cuaagggcuu caauaaaaag cggaucgacc accgacauca cacauuggau     3180

gcccugguua uugcugccug uacgaaagac caugugaacu auaucaccag cuuaaauacu     3240

gaaagaaaca accacucccu cgucucaaag cugcgcaaua ccaaaaaggu gcuuauaaac     3300

ggaaaggaga ggcguaucgc aaaagcguac aagcugccau ggagcaguuu uccccuagau     3360

gcuaaacacc agcucgagac aacaguaauu ucuuucaagc aaaaucugcg ggugaucaac     3420

aaauccagca auaagacuug gcaguggaga aacguuaaug gcgaacagaa gaaaaaguug     3480

auuccucaga ccaaagggga cucgugggcc aucaggaagu caaugcauaa agcaacgguc     3540

ucugccaagg uggagagugu gggaaagggu aaaauaaaga ccacaaaccg cacuccacug     3600

ggcgaaauua aaggggagaa gcacgucgag aaaaucuaug augcucgaau cagagaaguu     3660

auuauaccca aucauuuaaa caaguaccuc gacgagaagg gaaaaccgaa uuacgaagcc     3720

gcauuuuccc aaagcggcau caagcagcug aacgagaaua uugaagugcu uaacgagggu     3780

aaaccucacg cuccaaucua uaaggugccc cuguucgagg uagggucuaa auuuccuguc     3840

agcgaagacg ccaagucccc caaaaaucag aaguacgugg aggcggauaa gggcaccaac     3900

uuguucuuug ccguuuauug gaaugaagag aaacagaagc ggaacuucga aacagugcca     3960

cuccaucaag ucauugugca ccagaaacag guugcuucac ugccuacuga gcagaggacc     4020

agugugccca ucaagccaga gcuaggacug uuccuuuuua gccucucucc gaacgaccug     4080

guauacgucc cuacggaaga ggaaauagag aauccccacu ugguggaucu gaaaaacuua     4140

uccaaggacc aaacaaaucg caucuauaag auggugucaa guaccggcaa cgaaugccau     4200

uucauuccac accauaucag ccgucucauu augaaauaca augcaaagca gaaagucggg     4260

gaguuuggau cccugaacaa ggccgagaaa gcuaucacug gcgaaacaau aaaggagaag     4320

ugucuuaaaa uucagaucaa uucucugggu aaguugaaaa ccgaaagaau cgccuga        4377


<210>  3386
<211>  4380
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3386
augaaguuca accugggccu cgacaucggg accaauagca uuggaugggc ccuugugaac       60

gaggauuacg aaaauaaaaa ggguaaaaua aucgcugcag gcuccagaau uauccccaug      120

acacaggaca uucuggauaa guuugggucu ggagccauca cugagacgca aaccgcguug      180

aggacagacu aucggucaaa acgcaaguua auacagcgau uccugcuacg uagagaaagg      240

cuccaccggg uccugaacgu ucuugauuuu cugccuaagc auuacgcuga gaguaucgac      300

uucgaaaaau auuuuggcaa guucuugcca aauacugagc cgaaacucgc cuacaaccag      360

aaucaguuua uuuuccaaaa cagcuuucug gagaugcuga aggaauuuca ggagaaacac      420

cccgaacucu uaaccaauaa caagaagauc ccuuaugauu ggacaauuua cuaucugcgc      480

aaaaaggcac uuaaucagag aaucgagaaa gaggaauugg ccuggcugcu acuccaguuu      540

aaccaaaaga gggguuacaa ucagcugcgg gacgaggaag aggaauccgu gccaaacaaa      600

cagguagagu ucuacucucu uaagauaauu gauguggucg cugaccagcc caaagagggg      660

cgcgauaagc gaugguauuc gaucaaucug gaaaacggau ggaccuacag aagggaguca      720

aagacuccuu uguuuaacug ggaaggcaaa acaaaggacu ucauugugac cacggaucuc      780

aaucaagacg gcagcaucaa aaaggacaaa gaggggaagg aaaagcggag uuuccgugca      840

ccauccgagg gagauuggac ucugcugaaa aagaaaaccg agaaggaaau cuaugagucu      900

gguaaaacau uaggcacuua cauuuaugac gcccuccuua acacccccga ucugaagaua      960

aagggggacu uuauccgcac aauugauaga agcaucuaca aagaagaguu gcagcagaua     1020

auuaauaagc aaaaagaaua ucaucuagag cugaacuccu caaagcucua caaggcuugc     1080

cuggaggaac uuuacccuaa aaaugagauc uauaagagug cccugaaaaa caaggacauc     1140

gcguacuugu ucauuaauga uauccucuau uaccagaggc cacugaaagc accgaaguau     1200

aacauuggaa aauguaccua cgaaagccgg acguuuaaga aagaggacaa gaagauacga     1260

gaguauaucc ccgccguugc uaaaucucac ccuuuauucc aggaauuucg ccuguggcag     1320

uggauuaaga aucuaaaaau cuacgagaag cucuccucag auaacugcac ugacuucuuu     1380

cugacaaaug aacaagauuu cgagaaccuu uuugaguucu uguggaauaa aaaggaaauc     1440

gaccauaagg gcauucugga guaccucgug aaaaccaagu uuccagaccu gaaacccaag     1500

caggucaaaa cuaaggugaa ggaauauaga uggaacuacg uauaugauca cgagaaagac     1560

gaaagcaaga cauacccuug uggugagacc uauuuccuuc ugaaaaauaa guuggauaaa     1620

auauacaacg cccaggacga uuuucucgac aaggaaaggc uguauaacuu auggcacauc     1680

auuuacagug uuacggauaa gaaacaguac gagaaggcac uggcuaaauu cgcccuaaag     1740

aaucaucucg acaccaaauc uuucgugaac acauuuaagc ggaucccacc cuucaaaaau     1800

gauuaugcga uuuacucccu gaaggccauc aagaaacuuu ugccgcugau gcgcgucggg     1860

aaguauugga acuacgacga uauagacccu aucacuaaac aaaagauuca gugcaucauu     1920

accggagagg aaaaugauac aaucagcacu cucgugagag agaaaaccaa ccacuuuucg     1980

aaugaaaacg acuucaagaa ucugcccuua uggcuggcag gcuauauagu guacaacucu     2040

cauaaugagu ccucagaaau ugagaagugg aaaaguccac aggacaucga uacauuucuu     2100

aagaaauuca agcagcacgc uuugaggaac ccuauugucg agcaaguugu gaccgaaacu     2160

cuccguguag ugcgggacau cuggagcaaa aagggccagg ggaagaaaga uuucuucaau     2220

gagauacaca ucgaacuggg aaaggaccua aagaaccccg ucgauaaacg aagaaaacug     2280

uccucuacga auacaaagaa cgagagcacc aacacuagga uuaaggcccu ccugaucgag     2340

cuuuuuaccc agggugugga aaauguuaaa ccaaacucac ccaagcauca ggagauuuug     2400

aaaaucuucg aagagggcgc ucugaauagu gggauagaaa uugaggagga caucaucaag     2460

auuuccaaca cagaucaacc uaccaaaaag caguuagaac ucuauauccu guggcuugag     2520

cacaaguaca aaagcccaua cacuggacag ucuauuccgc ugucaaaguu guuuacaccc     2580

aauuaugaaa uagagcauau cauuccucgc aguaaauacu augacgauaa ccucagcaau     2640

aaggugaucu gugaaacgga gauuaaccgg cugaaaaaua accaacuagg cuacgaguuc     2700

aucaauaacc acgaaauaag agaggucacc cuggaagagg guaagacuau caggcuccug     2760

uccccagaac aguauuggaa uuuugugaca accuacuaug ccaaaaacaa ggcaaagcgc     2820

gagaaucuuu ugcugaaaga guuacccgua cagaugguug aaaagcagcu caacgacaca     2880

cgauacgugu cuaaauacgu caugcaacug cuuucgaaga uugugcggaa agaggauggg     2940

acugacaacg gcaucaauag caccaacauu cuguccaucu caggaaagau gacgcgugcc     3000

cuaaagaaug auugggaccu caacgacguc uggaaugauc uguugcugua uagauucgaa     3060

agguuaaaca ccauaacaaa aacuaaucuc uucaccacau acagugagca gaagcagaaa     3120

cgcaugccua uugugccaga ccaguaugcu aagggcuuuc auaaaaagcg gcuggaucac     3180

agacaccaug cgcuugacgc aaucguuauc gccugcauua cuaggaacca cauaaauuac     3240

augaacaauc aaaacaucca gggggagaag cugaaaaaga augcuauuga aaaagagaac     3300

uaucgagaaa ucuugcagaa gucucgccuc gauauucggg cccagcugug ugagaaaaag     3360

uacaaugaca acgaucccac caaguauaau uggguguuca ucaaaccgug ggcagacuuu     3420

acggaaaaca caaaggauau acuagagagc aucauuccuu ccuucaaaca aaaccugaga     3480

aucauuaaua agaccguaaa cuacuacgag aaauauaaug aaaacggaaa gaaagucaag     3540

gugaggcaga ucaaggguga caauugggcc auacguaaac cacuucauaa ggagacuauu     3600

ucaggcaaag uucaccucag cuacaagaca cccgcuaaag ggaagauccu gaccgccacu     3660

cgcaaggugu uggauucuuc cauggacuuu aaaaguaucg aaaagauuac cgacacagga     3720

auccagaaaa uacugaagaa cuauuuagag uacaaaaaga gcccugaacu cgcauucucu     3780

cccgagggca uugaggaacu gaaugcgacg aucaccaagu auaacaaugg uuuucaccau     3840

aaaccaauuu acaagguccg gaucuaugag gaagggguga aauucccucu uggcaacaag     3900

ggaaauaaaa aguccaagua cguagaggcu gaaaaaggca cuaaccuguu uuucgccaua     3960

uacaaggauc aagagaauaa cagauauuuu gagacaauuc ccuucaauga cguguuggaa     4020

acccucaaaa aggggauguc agcaguucca gagaaaaacc aggaagagaa cacacugguc     4080

uucgcccuaa guccgcagga ucugguguuu gugccuacuc uugacgagau cgaaaauccc     4140

gauaucgucg acuucaagaa ccucaccaaa gaucagacga auaggauuua caagaugguu     4200

agcucguccg gaaagcaaug cuuuuucauc aaagcugagg uggccacauc uauugugaac     4260

aagauggaau uuagcaccuc aaauaaaaug gagaacagua uagaagguau ccagauuaag     4320

uccaauuguu ggaaacugaa guuggaccga uuaggcacua ucacccgggu aaucaaguga     4380


<210>  3387
<211>  4383
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3387
augaccaagu ucaaccuggg ccucgacauc gggacaaaua gcauuggaug ggcccuugug       60

aacgaggauu gcgaaaauaa aaaggguaaa auacuggcug caggcuccag aaucauuccc      120

augacucagg acaucuugga uaaguuuggg ucuggagccg cgacggagac ccaaacagcu      180

uuaaggacug acuaccgguc aaaacgcaag cugauucagc gauuccuacu ccguagagaa      240

aggcugcacc ggguccuuaa cguucuggac uuuuugccua agcauuaugc cgagaguauc      300

gauuucgaaa aauaccucgg caaguuucug ccaaauaccg agccgaaacu gguguauaac      360

cagaaucagu ucauauuuca aaacagcuuc uuugagaugc ucaaggaguu ccaggaaaaa      420

caccccgaau uauuucugaa uaaccagaag aucccuuacg auuggacaau uuauuaccuu      480

cgcaagaaag cauuguccca gaagaucgag aaagaggaac uggccuggcu acuccugcaa      540

uucaaucaga agagagguua uaaccagcuu agggacgagg aagaggaaaa uaccccagau      600

aaacugguag aguuuuacuc uuugaagauu aucaaaguga cugcugacga ggcaaaggaa      660

gggauaaaca agcgguggua cucgauuaau cucgagaacg gauggaucua ucgccgagaa      720

ucaaaaacac cccuguucaa cugggagggc aagaccaaaa auuucauugu cacgacugau      780

uuaaaccagg acggcagcau caagaaagau aaggaaggga aggagaaaag aaguuuuagg      840

gccccuuccg agggagacug gacccugcuc aagaaaaaga cagaaaaaga gaucuacgaa      900

ucugguaaga cucuuggcac cuauauuuac gacgcucugu ugaauaaccc agaucuaaag      960

auaaaagggg cccugaucaa gacaauugac cggagcaucu auaaagagga acuccaacag     1020

auaauuaaua agcagaaaga guaccauucc gagcugaaca auucaaaacu uuauaaggcg     1080

ugucuggaag aguuguaccc caaaaacgaa aucuacaaga augagcucaa aaacaaggau     1140

aucgcauauc uguucauuaa ugacaucuua uacuaucaac guccucugaa agccccaaag     1200

uacaacauug gaaaaugcag uuaugagagc cgcaccuuua agaaggaaga uaaaaagaua     1260

acggaguuca ucccggcugu ggccaaaucu cacccccuau uucaggaguu cagacucugg     1320

caguggauua agaaucugaa aaucuacgaa aaacuuacua acgacaauua uacagacuuu     1380

uucuugacca acgaaaggga uaucgagaag cuguuugauu uccucuggaa caaaaaggag     1440

auugaccaua aaggcauacu ggaauaccuu aucaagacua aauuuccuga ucugaagcca     1500

aagcagguua aauccaaggu gaaagaguac cgguggaauu auauuuacga ccacgaaaag     1560

gacgagucaa agaacuaucc cuguggugaa acauacuucu ugcucaagaa uaagcuggau     1620

aaaaucacca gcauucaaga cgacuuuuua aacaagaaac gacuggagaa ucuauggcac     1680

aucauauaua gugucacgga uaagaaacag uacgagaagg cacucgcuaa auucgcccug     1740

aagaaccagc uugauaccaa gucuuucgug aauacauuua aacgcauccc ugcguucaag     1800

aacgacuaug ccauuuacuc cuugaaagca aucaagaaac ugcucccacu gaugagagua     1860

gggaaguacu ggaauuauga ugacauugau cccaucacua agcagaaaau acaaugcauu     1920

aucaccggag aagagaacga cacaauuagc acuuuaguua gggaaaagac caaucauuuu     1980

ucgaacgaga augauuucaa aaaccugucu cuuugguugg cuggcuacau cguguauaau     2040

ucccacaacg aaucaaguga gauagagaag uggaaaagcc cgcaggacau cgauacauuu     2100

cucaagaagu ucaaacagca ugcccugcgg aacccuauug ucgaacaggu ggugaccgag     2160

acucuacgcg ucguuagaga caucuggcug aagaaaggcc aagggaagaa agacuuuuuc     2220

aaugaaauuc acaucgagcu cggaaaggau cugaagaacc ccguggacaa aaggcguaag     2280

auauccucua cgaauagcaa aaacgagaca accaauauuc ggaucaaggc ucuuuugaaa     2340

gaacuguuaa aggaggguau cgaaaacauu aagccaaauu caccuaaaca gcaggagauc     2400

cucaagauuu uugaagaggg cauacugaac agugggaucg auauugagga agagaucauu     2460

aaaaucuccc agauagccca acccacuaag aaacagcuuc aguacuaucg aaauuggcug     2520

gaacaaaagu acaucagccc auauaccgga caguucauuc ccuugucuaa acucuuuaca     2580

ucaaaguacg agaucgaaca cauuaucccu agaaguaagu acuaugacga uagccugucc     2640

aacaaaguaa uaugugagac cgagauuaau aggcuaaaga acaaucagcu gggcuacgag     2700

uucaucaaca aucaugaaau ccgcgaaauu acucucgagg aagguaaaac aauacggcug     2760

cuuucuccaa agcaguauuu ggacuuugug acgaccuacu augggaaaaa caaggcaaag     2820

agagagaacc uguuacucaa agagaucccg auucaaauga ucgaaaauca gcugaacgau     2880

acuagguacg ucucgaagua ugugaugcag cuucugagca aaauuguucg caaggaggac     2940

ggcacagaua auggaaccaa cuccaucaau auacuaucag ugaguggcaa aaucacacga     3000

guccucaaga cugacugggg gcugaacgau guguggaaug acuugcugug cuaccgguuc     3060

gaacguuuaa acaagauuac caauacgaac cucuucacca cauacucuga gcagaaacaa     3120

aagaucauuc ccaucguacc ugaucaguau gccaaaggau uucacaagaa aaagcuggac     3180

cauagacacc augcucuuga cgcgauacug auugcaugua ucacuaggaa ucacaucaac     3240

uacaugaaua accagaauau ucagggugag aaguugaaaa acuucaagau cgauaaacac     3300

aauuaucaug aaauauuuca aaagagccgc cucgacaugc gggcccagcu gugcgagaaa     3360

aaguacaacg aucacgacuc caccaaauau acauggguuu ucacuaagcc augggcugau     3420

uuuaccgaaa acauuaagga cacgcuagag uucaucauuc ccucauuuaa acagaaucug     3480

agaaucauaa acaagacagu gaauuacuau gaaaaauaca acgagaaugg caagaaaguc     3540

aaggugaggc aaaccaaggg ggauaacugg gccauucgaa aaccucuuca uaaggagacu     3600

aucagcggaa aaguccaccu ccuguacaag acaccagcaa aaggcaagau cuugaccgcc     3660

acucgcaagg ugcuggacuc uuccauggau uucaaaagua uugaaaagau caccgacaca     3720

gguauucaga aaauauuaaa gaauuaucuc aaauacaagg agagccccga acuggcuuuu     3780

ucuccggagg ggaucgagga acuuaacauu acgaucgcca aguauaauga uggcuucuac     3840

cacaaaccua ucuauaaggu ucggauuuac gaggaaggag ugaaauuucc acugggcaac     3900

agagggaaua agaaaaacaa guauguagag gcagaaaaag gaaccaauuu guucuucgcg     3960

auauacaagg accaggagaa caauagguac uuugaaacua uccccuucaa cgacguccuc     4020

gagacacuga agaaagguau guccgcugug ccugagaaga accaggaaaa gaauacccua     4080

guuuuugccc ugucacccca agaucuugug uucgucccaa cacucgacga gauugaaaac     4140

ccugauaucg uggacuuucg uaaucugacu aaggaucaga ccaaccgcau uuauaaaaug     4200

guaaguagcu cgggcaagca guguuucuuu aucaaggcag agguggccac guccauaguu     4260

aauaaaaugg aguucacaac cucuaacaag auggaaaaua gcaucgaggg gauucagauc     4320

aaaucaaacu gcuggaaguu gaaauuagac cggcugggaa cuauuaccag agucaucaag     4380

uga                                                                   4383


<210>  3388
<211>  4389
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3388
augaccaaga acauccuggg ccucgaccuu gggacaaaua gcauuggaug ggcccugaua       60

aaacagaacu ucgagaauaa gcaaggugaa aucuugggca ugggguccag aauuaucccc      120

augucucagg auauuuuagg agacuuuggc aaagguaacu cagugaguca gacugcugag      180

aggacgaagu accggagcgu ccgccgacug cgugaaagau uccuacucag gcgggagcgc      240

cugcacagag uucuuaaucu guugaacuuu cucccucagc auuaugcauc ccaaaucgau      300

uucgagaaaa gguuugggaa guucaaggug gagaccgagc caaaacuggc cuggaagaau      360

auagacggac aguuuucuuu ccuguuucag acaucguuca acgaaaugcu cgaggauuuu      420

cggguaaaug gccaggacgu gaaaaucccg uacgauugga cuauuuauua cuuacgcaag      480

aaagcgcugu caaagaagau cgaaaaagag gagcuugcuu ggauuuugcu gaacuucaau      540

caaaagcgag gcuauuacca gcuaagaggg gaagaggaag aggaaaaccc caauaaacuc      600

gucgaguuuu auagccugaa ggugguugac guggucgccg augagccuca gaaaggaaag      660

uucgacaucu gguacagucu uauacuggaa aacgguuggg uguacaggcg guccucuaaa      720

auuccauugu ucgauuggaa ggacaagacc cgugauuuua ucguaacaac cgaccucaau      780

gacgauggca gcauuaaaaa ggacaaagau gggaacgaga agcgcuccuu cagagcaccc      840

ucagaaaacg acuggacucu gguuaaaaag aagacagagc aggaaaucga gcaaaguagg      900

aaaaccgugg gaacguauau cuacgaggcc uuacuggaua auccuaagca gaaaauuaag      960

ggcaaacucg uccggacuau agaacgaaag uuuuauaagg aggaacuucg ccagauccug     1020

gagaaacaaa aggaguucca ccaggaguug cagagcgacg aucuauacaa cgacugcgug     1080

agagagcugu auaggaauaa cgaagcucau cagcucaccc ugucuaaaaa ggauuuugug     1140

caccuucuga uggacgauuu gauuuucuac caacggccac uccgcuccca gaaaucaagc     1200

gucaguaauu guacacugga guuuagaaag uauaaggacg aaaacggugc agagcauacu     1260

caguacuuaa aagccauccc caagucuaau ccuuacuauc aggaguucag gcuguggcaa     1320

uggauauaca accuaaauau uuaucgucgg gaagacgagc agaacccaau caccggggaa     1380

uuucucucca caaccaaaga guucgaaaau cuguuugagu uccuuaacac gcgaaaggag     1440

guugaucaga aggcuuugcu gaagcacuuu aaacucaaug acaagacuca cagauggaac     1500

uucguggaag auaagaaaua cccgugcaau gagacaaaga ccaugaucag cucgaggcug     1560

gaaaaaguag agaacauuuc ugacgauuuc cuuacucgcg aaaucgagca gaagauuugg     1620

cauauaaucu auuccgugaa cgacaaaauu gaguacgaaa aggcccugaa gucauuugcg     1680

aaaaagcacc auuuggauga gaguagcuuc uuugaagcau uccggaaauu ucccccuuuc     1740

aaguccgagu auggaucuuu uagcgaaaaa gccgucaaga agcuccugcc auuaaugaga     1800

cugggcaaau acuggaauua cgcugacauc gauaagucaa guaaagagag gauccaaaag     1860

auuauauccg gcgaguauga cgaaaacauc aaagaucgcg ugcgagaaaa gagcguucac     1920

cuaacaaccg aaaaugacuu ccaggggcuc cagcuguggc uugcccagua cauuguguau     1980

ggacggcacu cugaggcauc aaugaucggu aaguggaaca gugcugauga cuuggaggaa     2040

uuucugaaaa auuucaagca acauagccuc cguaacccca uugucgaaca ggugaucacg     2100

gaaacccuga gaguaguuaa agacauaugg uuaaaguacg gcaauggggc caaagacuuu     2160

uucaacgaga uccacauuga acugggaagg gagaugaaac uuccugcgaa agagcgcgaa     2220

gauuugacac ggaagaucac ugagaaugaa aacaccaauc ucagaauuaa ggcccugcua     2280

gcagagauga ugaacgauuc cucuguggaa aaugucaggc cauuuucgcc caugcagcaa     2340

gagauccuga aaauauauga ggacggcgug cucaacagcg guauugaaau cgaggaugaa     2400

uaccugaaga uuuccaaaac agcucagccg ucaccuagug accuuaagcg auauaaauug     2460

uggcuggagc agaaguacaa gucucccuac acugggcaga ucauaccauu aaauaaacuc     2520

uucaccccug aauaugagau cgagcauauu aucccccaaa gccgcuacuu cgaugacucc     2580

uuuucaaaca agauuaucug ugaaagcgcc gucaacaaac ugaaggauaa uuauauaggc     2640

cuuggauuca uuaaacaguu uggcgggaca aucaucgagc ugggauucgg uaagucugug     2700

aagguuuuug aaaccgagga guacgaagac uucgugaaaa agcacuaugc uaacaaucgg     2760

uccaaaagaa acaaguugcu ccuggaggau auuccagaga aaaugaucga aaggcagauu     2820

aaugacacuc gucauauaag uaaguacauc agcggcguac uaucuaacau uguccgcauc     2880

gaugacgacc aggccaagaa aauuaucgaa gaugcagaga uacuggcccg gaaggagauc     2940

gacaaagaua aggcuaauga aauuagaagg aaagcgaagu cccucgcaac ggacgauggg     3000

aucaacucaa agaauauugu gcccggaaac ggcaaaauca caacccaacu gaagcaggac     3060

uggggucuua augauguuug gaacgacuug auacugccuc gauuugagcg gaugaaucag     3120

uuaacuaaca guaaagauuu caccgugugg aaugaaaacc accagaagua ucucccaaca     3180

gucccgauug aguuuagcaa aggguucucg aagaaacgca ucgaccacag acaucacgcc     3240

cuggaugcuc uugugaucgc cugcgcaacc aaggaccaug uaaaccugcu aaauaaccaa     3300

uccgccaagu cugacacuaa aagguacgau cucaagaaaa agcugaugaa auuugaaaag     3360

gugguuuacc accacacaca gacgggcaau aagauugagc gggagauacc caaacaguuc     3420

uugaagccuu gggaaaacuu caccuuugac gcuaaauaua aucuggagag caucauuguc     3480

ucauucaagc aaaacuuacg agugaucaau aaagccacua acuacuauga aaaguacgug     3540

gagaaggaug gaaccaaaaa uaagggcaga gucgaacaga cagggacaaa cugggcaauu     3600

cgcaaaccaa ugcauaagga cacuguuagu ggaaaaguga aucuccccug ggugaaggua     3660

ccuaagggua aaauccugac cgcgacgagg aagauacuug auaccuccuu ugaccugaaa     3720

ucuaucggca gcauuacaga uacugggauc cagaagauuu ugcguaacua ucucgaguuc     3780

aaaggauccc cagagcuggc uuuuucaccc gaaggcaucg aggaccuaaa uaagaacaua     3840

gaaaaauaca augaugguaa gccgcaccag ccuauuaaca agguccgggu guucgagcug     3900

gggagcaaau uucaaguugg ccagaccgga aauaagaaag acaaguaugu ggaggccgca     3960

aaaggcacaa accuuuucuu ugccgucuac gaagauaaga acgggaagag aaguuacgag     4020

acuaucccac ucaaugaagu gaucgagagg cagaaacagg gacugucugu uguggacuug     4080

aagggcacca acgauuucua ucuguguccc aaugacuuag uauacauucc uuccgacgau     4140

gaacucgaga acaucaauaa cauagacuuu aaaaauauug cuaaggagaa aaacgaacgc     4200

aucuauaagg ucgugagcuu cucggguuca caaaucuuuu ucgugcgaca ggauauugcc     4260

acgucuauag ucaauaaggc agagcugagu acacuuaaca aaauggaaag agcuauggac     4320

ggguccauga uuaaggagag cugcguuaaa cugaaugugg aucgguuggg aaacaucucu     4380

aaggccuga                                                             4389


<210>  3389
<211>  4401
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3389
augaccaaga acauccuggg ccucgaccuu gggacaaaua gcauuggaug ggcccugaua       60

aaacaggauu ucgaaaacaa gcaaggugaa aucuugggca ugggguccag aauuaucccc      120

augucucagg acguguuagg agaguuuggc aaagguaauu cagucaguca gacugcugca      180

aggacggauu accgcggggu ucggcgacug cguaagagau uccuacucag gcgggaacgc      240

cugcacagag ugcuuaacgu acugaacuuu uugccugugc auuaugccag ccagauugac      300

uucgagaaaa gguuuggaaa guucaaggaa gauaccgagc caaaacucgc guacaauaag      360

gagggcuuua ucuucaaaaa uuccuuugaa gagauggucg cugacuuccg gaagcaccaa      420

ccgcagcugc uggaaaacga uaaaaagaua cccuaugacu ggacaaucua cuaucuccgc      480

aagaaagccu uaucuaagaa aauugagaag gaggaacugg cauggauccu uuugaauuuu      540

aaccagaaac gaggcuacua ucagcugaga ggggaggaag aggaagagaa uccuaacaag      600

cuaguggagu ucuacucgcu caaaauuacu gauguucugg ccgacgaacc acaaaaggga      660

aagucagaua ucugguacag ccuuauacug gagaaugguu ggauuuauag gcggaguucc      720

aaaacccccu uguuugacug gaaggagaaa acacgugauu ucaucgugac cacugaccuc      780

aacgacgaug gcucuauuaa gacagacaaa gaugggaaug aaaagcgcag cuucagagcu      840

ccuuccgaaa acgacuggac ccuggucaag aaaaagacgg agcaggagau cgauaaauca      900

cagaagacug ugggaaccua caucuaugaa acauuacugc agaacccaaa acaaaagauu      960

aagggcaaac ucguaugcac uauagagagg aaguuuuaca aagaggaacu uaagcagauc     1020

cuggaaaaac agaaggaguu ccaucaagag uugcagaaug acgaucuaua uagugacugu     1080

guucacgaac uguaccggac caacgaggca caucagcuca cacugagcaa gaaagaaauu     1140

gugaaucuuu uuauggagga ucugaucuuc uaucagcgac ccuugcgcuc ucaaaagacc     1200

uccauaucaa acugcacgcu cgaguuuaga aaauacaagg acgaaaaugg ugucgagcac     1260

acucaguucc ugaaagccau uccuaagagc aacccauacu aucaggaauu uaggaucugg     1320

caguggaucu ucaauuuaaa cauuuacaaa cgggaugacg acaguaaugu gacaaaggag     1380

uuucugaaca ccacugaaga uuucgagaau cuauuugagu uccucaacaa ucgcaaggaa     1440

guggagcaaa aagcucugcu uaagcacuuu aaauugaacg aaaagcugca uagauggaau     1500

uucgucgagg acaaaaagua uccguguaac gaaacaaaga ccaugaucuc uacgaggcuc     1560

gauaaaguug agaacauuuc caaggacuuc uuuacccgug agauagaaca gaaaaucugg     1620

cacauuaucu acagcgugaa ugauaagauc gaguaugaaa aagcccugaa gucguucgcg     1680

auuaagcaua accuugacga gaauagcuuu uucgaagcau uuaaaaaguu ucccccuuuc     1740

aaauccgagu acgggucauu cagugagaag gccaucaaaa agcuguugcc acucaugcgg     1800

cugggaaagu auuggaacga agaugagaua uuaaaauaca gcgacgauua cuuuaagaau     1860

auugaauccc ugcuaggcgu acucaaacag cgagacgaga acaucucuga ugcugacaga     1920

gagaauuaua acaagacaau uaauaacaaa cugagggccg aacuugagaa uuuccagaac     1980

gcagaaaucg agagcuuuca aggcuugcgc cugcacauag cucaguacau ccucuauggg     2040

aagcacucag aaggagauaa uaaaaaguuc aacagugccg acgaccugga gaaguuuuua     2100

aaagaguuca agcagcauuc ccugcggaau cccauugugg aacaggucau cacugagacc     2160

cuuagagugg uuaaagauau uuggacaaag uacgguaacg gcgcgaaaga cuucuuugau     2220

gaaauccaca uagaguuggg gagggacaug aagaagacuc gcgaugaacg agccgacgca     2280

accaaaauua ucacagagaa cgagaauacc aaccuccgga uuaaggcucu gcuagccgaa     2340

augaugaaug auguggagau ggaaaacguc cguccuuaua gcccaaugca acaggagauc     2400

cugaaaauau acgaagacgg aauccucaag ucugauauug agaucgagga cgauauucug     2460

aaaaucucaa agacugcuca gcccaguagc uccgaccuua agagauauaa auuguggcug     2520

gaacaaaagu acaaaucucc guacacgggc cagauaauuc cuuuaaauaa acucuucaca     2580

cccgaguaug aaaucgagca uaucauucca cagucgaggu acuuugauga cagcuucucc     2640

aacaagauca uuugcgaguc agccgugaau aagcugaagg acaacuauau aggucuuggg     2700

uuuaucaaac aguucggcgg aaccauugua gaauguggcu uugggaagaa uguuaaagug     2760

uucgaggcag aagaguacga agauuuuguc aagaaacacu augccaacaa ucgcggaaag     2820

cggaacaagc uguugcucga ggagaucccu gagaaaauga uugaaagaca aaugaaugac     2880

acuaggcaua ucaguaagua cauaucuggu gugcugagca acaucguccg aguggaagau     2940

ggcuccgacg aggggguuaa uagcaaaaac auugugcccg gaaacggcaa gaucaccaca     3000

cagcuaaaac aggauugggg ucugaaugac guauggaacg aacucauucu gccacgcuuc     3060

gagcggauga aucagcuuac caacucaacu gauuuuacag cuuggaauga gaaccaccaa     3120

aaguucuugc ccacgguccc uaucgaguuc ucuaaagggu uuuccaagaa gagaauagac     3180

cacaggcauc acgcgcugga ugcauuaauu aucgccugcg cuaccaaaga ccaugugaau     3240

cuccugaaca aucagagugc caagagcgau acuaaacguu augaccuuaa gaaaaagcug     3300

augaaguucg aaaaaguugu guacaaccac ccacagacag gcgaccgcau cgaacgggag     3360

gucccgaagc aguuucuaaa acccugggau aauuucaccu uugaagcaaa gaacaaucuc     3420

gacaaaauua uagugucuuu caagcaaaac cugagaguaa ucaauaaggc cacaaacuac     3480

uaugagaaau uuguggagaa ggauggaguu aaaacuaagg aaagggucga gcagaaaggc     3540

accaauuggg cuauucgaaa guccuugcac gaagagacgg ugucagggaa gaucgaccug     3600

ccuugggugg agaccaacaa cggagaguuc auuacagcca cucggaaaag uuuagaugca     3660

ucuuuuaauc ucgaaaagau caaaaagaua accgacacag guauccagaa aauucugcgc     3720

aacuaccuua aggcgaaaga ugacaaugcu gaacuggccu ucucgccaga gggcaucgaa     3780

gauuugaaca agaauauuga gaaguauaac gacgggaaau cccaucaacc caucgucaga     3840

guuagggugu acgagaaggg aaaaggccgg uuugcacucg gucagacugg gaauaacaua     3900

gauaaguaug ugcagggcuc uccuaaucug uucuuugcca uuuacaaaga cgaaaacggc     3960

aaaaagagcu augagucaau ccgacuagau aucguaauug aaagaaugaa gcagggacug     4020

caagcugucc cagagaccaa ucccaacggg aucagucuuu accagaaucu cuccccgcug     4080

gacuuggugu acauaccuac ggaagacgag auugagucuc cacaccugau cgauuucaca     4140

auuuuaaaca agacccgccu cgaaaggauc uauaauguua acgacuucag cggauacacu     4200

auauauuuuu cccagaacuc auucgccaaa aauauuuacc ccaaggagau ggauaugaca     4260

uggaacgaga aaaagcagaa gccuagcggc aguuuugacu cuaaaaccgc auccuauaau     4320

aagaaaagca ucaaggauau cuucauuaaa cugaagaucg accgucuugg uaacauuucg     4380

aaggugucaa auacucugug a                                               4401


<210>  3390
<211>  4401
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3390
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugggc ccuggugaaa       60

gaggauuucg aaaacaagca gggugagaua uugggcuuag gguccagaau cauucccaug      120

ucucaagaca uccugggaga auuuggcagg gguaauucaa uuagucagac agcugaucgg      180

acuggguacc gcggaguccg acgucuaaga gagaggcacc uccugcggcg cgaaagacuu      240

cauaggguuc ugaacaucuu gggcuuccuc ccuaaucacu augcaaaaga gauagacuuu      300

acgaagcggc ugggcaaauu caucaaccau gccgagccaa agcugagcuu ugauaaggag      360

uucauuuuua aagacuccuu caaugaaaug cucaaggauu uucagcagaa ccaaccgggg      420

uucaucauua auaaacacgg agaagaggcg uuagugcccu acgacuggac caucuauuac      480

cugcgcaaga aagcucuuac acagaagaua gauaaggagg aauuggccug gauucugcua      540

aacuuuaauc agaaacgagg uuauuaccag cucagaggcg aggaagagga agagaacacu      600

aauaagcugg uagaguucua uucucuuaaa aucauugacg ugaucgcaga uaacgaaguc      660

aauaagaaag gggaggugug guacucgcug cauuuggaaa acggauggau cuacaggcgg      720

ucaagcaaga uuagucucgu ugacuggaaa gauaaggugc gugacuuuau agucaccaca      780

gacgugaacg augacggcuc cgagaagcug gauaaagacg guguaguuaa gcgcucuuuc      840

agagccccua gcgcugauga cuggaccuua cugaaaaaga aaacugaaca agauaucgac      900

aauuccaaca agacaguggg ggcauauauu uacgagaauc uccuucugaa cccaaagcag      960

aaaaucaagg gaaaauuggu caggaccaua gagcggaagu ucuauaaaga ugaacuaaag     1020

cagauucugg agaagcagaa agaauuucac caagagcucc agaaugaaca gcugcuucaa     1080

gacugcgugc gagagcugua caaguauaac gagcagcacc agcagauguu ggaaucaaaa     1140

gauuucgugc aucucuuucu gaaugacauc aucuucuacc aacgccccuu aagaagucaa     1200

aagagccuga uuucuaacug uacgcuagag aaaagggucu cccgggacgg cguuguguuu     1260

ccuaucaagg uaauuucaaa gagcaaucca uauuaccagg aguuccgccu ccugcagugg     1320

cuucaaaacu uggccauaua cagaaaagau gacgauguga augucacuca ggaauuucug     1380

aacaguaucg agucuuggga aaaucuccug caguuccuua acgcuaagaa agagauuaag     1440

caggaacaac ugaucaaauu uuugcucgag cagaagggcc ugaaaggaaa uuccuuaaag     1500

accgccaucc ugacauauag guggaacuac gugacugaca agguuuaucc caugaacgaa     1560

acccguagcc uaauucagga uaaacucaag aaaguggaga augucgcgac agacuuccug     1620

ucguuugaua acgagauggc acuuuggcac auaaucuacu cugugaauga caagauugaa     1680

uaugagaaag ccuuggcuaa guucgccucc aagcauaacc uggaugaaau cucauuugua     1740

gagaguuuca aaaaguuccc uccauuuaaa agcgacuacg gguccuucuc ugaaaaggca     1800

auuaaaaagc uccugccguu aaugcgguuu gguagcguuu ggaauaagaa caauaucgug     1860

cccucagucc uggaucgaau agacaaaauc cuuaccggcg aguaugauga gaagauuaaa     1920

aucagaguga gggaaaaggc ugagcaguuc caauugaacc agauugaaga cuuucagaau     1980

cucccucugu ggcuagccca auacgucgug uacgaccgcc acagugaggc ggaaaucgcc     2040

gggaaaugga acuccguuaa ggaucuggag aauuaucuca aggaguucaa acagcacagc     2100

cugcggaacc caauagugga acagauuauc acggagacuc uuagaguagu caaggacauu     2160

uggcagcaau acggaaaugg cgugcagaac uuuuucgaug aaauccauau agaguugggu     2220

agggagauga aaaauacagc agacgaacgc aagcgaauca ccaacucugu uacugagaac     2280

gaaaauacaa accugcggau uaaggcuuua cucguggagc ugaaagaaga uaccaauauc     2340

gagaacgucc guccccacuc accuaugcag caggagauuc uuaaaaucua ugaagagggg     2400

gugcugaaug ccguagaaaa cauagacgag gauauuuuga agaucaguaa gacggcucaa     2460

ccaagcaaau ccgaacucau cagauacaag cuguggcuag agcagaaaua ucagucuccc     2520

uacaccggcg agaugauucc gcugucgagg cucuuuacaa gcgacuauga aaucgagcau     2580

gugauuccuc agucccgcua cuucgaugac ucacugagua auaagguuau augcgaagcc     2640

gcagucaaca aacggaagga uaaucaacuu ggauuggagu uuaucaagaa ccaccauucu     2700

gagaaaauug aacugggcaa ugggagaacu gcccaggugu uagaggugga agcguacgag     2760

agcuucguca agaaaaacua ugacaagaau aggggaaaac gaaacaagcu ccugcuugaa     2820

gauguucccg agaagaugau cgagcgccag cugaaugaca cccgguacau uuccaaauuu     2880

gugacacacc uacucucaaa caucgugaga aaggacgaag uaaacagcaa agaugacggu     2940

auaaauucua agaacaucau uccaggcaau gggaaaauca cuucccaacu gaagcaggau     3000

uggggauuga acgacgucug gaaugagcug auuuuaccua gguucgaacg uaugaacaau     3060

cucaccaaca guacagauuu caccagcacu aauacgaacg gcaagaucau acccacagug     3120

ccacuggagu auucuaaagg uuuuuccaag aaacgcauug accaccggca ucacgcucuu     3180

gaugcacugg uuaucgccuu ggcuaccaga gaccauguga aucuccugaa caaugaauca     3240

gcccuaccca agaaagauag gacuaaggag gagcagaaga guuuccgauu ugaccugcag     3300

gucaaccuuc ggaauaaaga aaaguacuuc aacgugaaag aggggcgcga aaaggagaaa     3360

uuuaccacau ucaagaaacc uuggaauacc cucacucaag auacaagaga cgcacuggaa     3420

aacaugguug ugagcuuuaa gcagaacuug aggguaauca auaaggccac gaacuauuac     3480

gagucguaua ccgacgagaa uggcaaccug cgguuagaua aauacggaaa uccaaagaaa     3540

gaacucauuu cccagaaggg cacuaacugg gcuauacgaa aaucucugca caaggagaca     3600

gucagcgggc agauccaccu ugacagagug aaggugacca aaggaaagau ugucacagcc     3660

acucgcaaau cacuggauag uuccuucaau gaaaagucua ucgagagcau uaccgacacg     3720

gguauccaga aaauauugag gaacuaccuc aaggcaaagg auaauaaacc ggacguugcg     3780

uuuucccccg aaggcaucga ggagcugaac aagucaauua gcguguauaa ugaagggaaa     3840

aaccaucagc cuaucauuaa gguacguauc uucgagcagg gaaguaaauu uccagugggc     3900

gaaaccggua auaagauagc uaaguucguc gaggccgcaa aagggacaaa ccuauucuuu     3960

ggcauuuacc aggauaagac uggaaaacgg ucuuaugaca ccaucccccu gaaucuugug     4020

aucgagagac ucaagcaagg ccuguccccu guuccagaaa caaacgagaa agggcaccag     4080

uuguuauucc aucugagccc caacgaucuc guguacgucc cgacuauuga cgauaaugaa     4140

cagcuggacu cgaacagggu guauaaguuu accgauucau cugacacgac auguaauuuc     4200

aucccuagua acauauccgc cauuaucuuu aauauuaaca gcaaagagca ggaaaagcgc     4260

ggaaucaauu acccaauaca aaacgagcuu ggcguugguu cuccccagag caagaaucag     4320

aaauccuucg acggguuuau gauuaaggag gugugcauca aacugaaggu cgaucgauug     4380

ggaaacaucu caaaaaccug a                                               4401


<210>  3391
<211>  4416
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3391
augugcaagu acuucuauau caaaaacuuu auuaccuucg ugcugagcaa guccauaauc       60

cccaugaaac acauucucgg ccuugaccug gggucuaauu caaucggaug ggccuuuguc      120

cagcaagauu ucgagaacaa gcaggguaaa auuaucgcua caggcaguag aauaaucccu      180

auggaccagg ggauuuuggg agauuuugaa aggggcaaua cuguuagcca gacggcagag      240

cggaccacau accgcuccac ucgacguuua agagaaaggc aucugcuacg gcgcgagaga      300

cuccacaggg ugcugcauau ccuugguuuc cugccaccgc acuaugacgc ccaaauugau      360

uuuaccaagc gguacgggaa guucaucgac aacgcggaac ccaaaauagc uuauaauaac      420

ggaaauuuua uuuucaugaa cucuuuuaau gagaugaucg aggauuucaa gaaacaucag      480

ccucaguugu uuuacaagaa aucgaacggc gaagagucaa agauuccaua ugacuggaca      540

aucuacuacc uccgcaagaa agcccugagc cagaagauca cccaacagga acuggcaugg      600

cucauuuuac acuucaauca gaaacgaggc uauuaccagc ugagagggga ggaggaaacu      660

gagaacccca auaaggaagu agccuuccac agucuuaaag uggucgaugu ggaggcugaa      720

gcaccuaaca agaaaggaga gauaugguau acaaucaggu uggagaaugg uuggauuuac      780

cggcguaccu ccaagauccc acuggacgau uggaagggca aaacgcgcga cuuuauaguu      840

acuaccgauc uaaacgacga cgggacaauu aagguggaua aagaaggaaa ggagaaaaga      900

ucuuucaggg cccccgacga aaacgauugg aaucuccuga agaagaaaac ugagcaugac      960

aucgauuuua gcggcaagac cgucggugcu uauaucuacg accaccuucu gcaaaacccu     1020

aaucagaaaa uuaaggggaa auuggugcgg acaaucgaac gaaaguauua caaggaugag     1080

cucaaagcca uucuggcgaa gcagauagca uuacaaccag agcuguucac cgaaacgcuc     1140

cuugccgacu guauccgcga gcuguacagg aaaaacgaaa cucagcagca gaauuugcua     1200

uccaaggauu uucugcaucu cuucguagag gacauuaucu uuuaucaaag accgcugcgg     1260

agcaaaaaga guacaaucgc uaacugcacc cuugaaaagc gcagcuacau ugauaaagag     1320

acuaaugaga gaaaggacgc ccccauaaaa guuugugcaa agucuaaccc uuauuaccag     1380

gaauuuaggg ugcugcagug guugcagaau cuccguaucu augagauuga aacagaccgg     1440

gaggucaccg cugaguuuau cgaaacgcca caagauugcg aggcccuguu caacuuuuua     1500

augucccaga aagaaauuga caauaaggga cugcuaaaau acuuccucuu ugcgaagaac     1560

ccccagauca aggauaaagc cgcaaaguca gagcugaaaa aguggcuuac cacauaucga     1620

uggaauuacg uguacgacgc ugccgauaac agcaguaaaa aguauccuau gaaugaaacu     1680

ggcuacgagu ugcagagaua ucugcaaaag guggagggcc ucccagcuaa cuuccugacc     1740

uucgacauaa cauaccagcu uuggcaccug uuguauucug ucaaagauaa gguugaauuu     1800

gagaaagccc ucaagaaauu cgcaaagaag aaacagcugg acaagacuuc cuuuguggau     1860

agcuucaaaa acuuuaagcc cuacccgaau gaauaugggu cguucucuga gaaagccauc     1920

aagaaguuac ugccucuaau gagguuugga aaccacuggg acuucaaucg cauugaugua     1980

uccacccaga aacggaucga cgaucucauu aacggugugg cuaaugaaga caucagaaca     2040

auaauuaggg agaaggcgga gaaauaccaa cugaacucag aaaccuguuu ucaggaccuu     2100

cccuuguggc uggcacagua cgucguguau aaucgccaug ccgaggcuac ugauaucgaa     2160

aaguggacga guguuagcga ccucgaggcc uaccuggaag auuucaaaca acacuccuua     2220

cgaaacccaa uuguggagca ggucgugaca gagacccugc ggguaguucg ugacaucugg     2280

cagcaguaug gcgggggaca agcaaauuuc uucgaugaaa uacauaucga gcuuggcaga     2340

gaauugaaga aaacugccga ggagaggaag cagcucucug acaagaacca ggaaaaugag     2400

agcaccaacc ugcgcauuaa aaagcuacug gcugaacuca aagaggaugc cucaauccag     2460

aaugugcggc cuuacagucu gguccaacag gaacuuuuga agauuuauga ggagggugug     2520

uuuuccgcaa cagacgaagu cgaggaagau auccagaaaa uaagaaagaa ggcggagccc     2580

agcaaagcug accugcagag guacaaguua uggcucgaac aaaaauauaa gucuccauac     2640

accggggagg ugauuucacu gacuaaacuu uucacagagg aauacgagau cgaacacauc     2700

auuccccaga gucgauauua cgaugacagc cuguccaaca agguuaucug cgagucugcc     2760

gugaauaagu ugaaaacgaa ccagcucggc cuggaguuca uucaaaacca ucacggagag     2820

aagguacgca uaaccgcaaa uaaagagguc acuaucuuua cagaagaaca guauaaggag     2880

uucgugaaaa agcacuacgc caacaauucg gcuaagcagc agaaacuacu gcucaacgaa     2940

auuccugaga agaugguugc ccggcaacug aaugauacca gauauaucag caaauacauu     3000

uccgaaaucc uuucaaagau agugaggagu gacgagaaag acgaaggcgu caacucuaag     3060

aauaucauuc cauguacagg gaagaucacu accgcauuga aacaggauug gggacugaac     3120

gacgugugga auaaguuaau ucucccgcgu uuugagcgca ugaaccagcu gacgcagacc     3180

caacuuuuca caagcuauaa ugaucggcug cagaaaaacc uacccacugu accuaucgaa     3240

uacucccagg guuuucagaa gaaaagaaua gaccauaggc accaugcuau ggaugcccuc     3300

gugauugcgu gcgccacccg agagcacauc aauuacauga acaaucaaca cgcucuggac     3360

aagucaaaag auaagaagga gaaacagcag agccggaucg acuugcgcac aauucugugu     3420

gaaaagaaaa acaacaauug gcaguucaag aaaccauggu cuacuuuuac ccaagaggca     3480

aaggaagccu uagagcauau aguugucucc uucaagcaga accucagagu gaucaauaaa     3540

gcuacgaacu auuacgaaaa gugggaggag aaaaauggcg ugcuggucaa gaaaaagguu     3600

aagcaggaag ggacaaacug ggccauuagg aaaccccuuc acaaggagac cgugagugga     3660

aaaaucgugc uggaucggaa gauuggcaaa gacaagaucu ugacugcaac acgaaaggcg     3720

guagauauaa ccuuuacuga gaaaaucauu agcucuauca ccgacacagg uauucaaaag     3780

auccuccuga auuaucuaaa auacaagggg uccccugaag ucgccuucuc aacggaaggc     3840

cuggaggagc uuaacaaaaa uauagcucag uauaacaaug gaaagaaaca ucagccaauu     3900

uacaagguga gaaucuuuga agagggcagu aaguuccccc ucggggaaac cggagccaaa     3960

gcaacuaagu auguugaggc ugccaaaggu acaaaccugu ucuuuggcgu guaccagggg     4020

aagggaaaac gcaccuacgc aacaaucccg uugaaugagg ucauugaaag gcaaaagcag     4080

ggccugccua gcgugccaga gcguaacgaa aagggugagc ccuuacucuu cucgcugucc     4140

ccuaaugacc uuguuuaugu gcccauggaa ggggagaucg ccgagacuau agauuuuaac     4200

aaucugucua aagaacagaa ggagcggauu uacaaaaccg uaucauucac gggcaaccag     4260

ugcuuuuucg uccaagaagc uguggcgaca ccaaucguga acaagaugga guauucaccu     4320

uugaauaaaa uggaaagaga cauucucgga accaugauca aggaggucug uauaaagcug     4380

aaaguugaua ggcuaggcaa cauuacuaag gccuga                               4416


<210>  3392
<211>  4416
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3392
augggcuaca aggcccccag aaacgacaaa ggggagaucg auuauggagu gggucuggac       60

cucggcaaug ggagcgucgg auggguugcu auggauaagg acuaccaccu uauuagggca      120

cggggccaug aacugauagg ugcccgcuug uucgauccug cgaacuccgc ugagaaucga      180

cgugaaucua gaaccacaag gcggcgcuua ucaagaaggc gguggcgccu gcgaaugcua      240

gacagucucu uugccagcga gcuggcaaaa aucgauccaa acuuccuugc cagaaggaag      300

uauuccuggg ugcacccgaa agacgaacag aaucaugaga acugguacgg gucuauuauc      360

uuuggaucga aggagcaaga uaagcaguuc uauaaagacu accccacuau uuaucaucug      420

cggaagaaau ugacggaaga uaccgagcgu cacgacaucc gcgaaauaua cguagcuauc      480

caccacauug ugaaguacag aggccauuuu cucagggagg gcgagaucaa uucagaaaac      540

cuguucgauc cugagggguu ugucgaacug auuaaagacg ugaucaagcu caauaacagc      600

uaugacgaga guacaccaau auucgaagca ucccccgccg auguuucuga ggcuuuacug      660

aaugagcaca cuagccgguc cggacgaauu gaagacaccc uugauaaguu ggacuuuuca      720

gugagucgcg ucgauaaaca ugagcugaag gguaugagag acgccgcgaa agcagugauc      780

ggcgccauug uagggaacac agcugauuuc agcaagaucu uuaaucuaga caaccucgau      840

aaagacucua aggauaaucu gaaacuuaag uucgccgacu ccaacuuuga cgaaaaguac      900

ggaggcguug uggguucagg gcuguuggau gagacccaga ucgaacucgu cgacaggauu      960

cacgcagugu augaugcugu ggcccugaaa ggaauauuag gcgacgcgca cacuaucagc     1020

gaggccaagg ucgagaguua cgaaaaacau cggaagaacu ggaaacugau uaaggaggaa     1080

cuccgcacaa aggagaauaa agaugaaguu gaccuuuggu auggcgaucu gucugggaga     1140

caguacaaga acccuccaga ccccgagaaa ggaccugaug aggguuccaa gaaagacagc     1200

ccaaucaggg auucggacca aaagcagaau uugauaaaga aagcaaacga uuauuucacc     1260

gaacuaauug acgacucuca gcuguccgag gaacagaaga auugccucaa aaacgauauc     1320

aauaacggcc aacuguuccc ggugcagcgu ucaagugaca augggguaau ccccuaccag     1380

auucaccuua acgagcugcg gcgaaucauu gagaaucaga agcaauacua uccuuuucua     1440

gcugaaacgu ucauaagaaa aacugccgag ggaaagagcu acgaagagaa caagaucgug     1500

gcucuccuga aauuuagggu cccauauuac gugggccccu uaguuuccaa ggaagauaug     1560

gaggagcauu cuagcgguaa uucagccuau cacuggaugg cacgcaaccc ugggcggggc     1620

gaagccauua ccccauggaa uuucgacgag guggucgaua aagacgaaag uggagcuaga     1680

uuuaucucca ggcugacagg cacugauacc uaccuacucg gggagcccac acugccgcag     1740

cauagccuuu uguaugaaga guacauggug cugucugagc ucaacaacgu acgccugaau     1800

guucgacagg gaaaccacug gucagacaag aaucggcaac gucuuaguua caaacagaag     1860

gaguuccuga ucgaaaacuu guuuaaagaa cacaagaccg ugacgagaaa gagggcggag     1920

gauugucuca gcuccauggg uugcggcgaa guccagcugu ucggguuauc ugacgagaaa     1980

aaguuugcau cgagccuguc cucauauauu acuuugaaaa agauacucgg aaaagccuuc     2040

guggaugaca guggcaauuc ugcuauccug gagcgcauua ucgaagucca gacaguguuu     2100

gaggaucggg aaccucuuac ccaucaauug agccugauug acucccucac uguugagcag     2160

agaaagcagc uggccaagau ccacuacaca gguuggggga aauuaucaag gaagcugcuu     2220

accagcaaaa agggcucuua uaaaauacau ucccagaacg agaucaugcg aacgcaacac     2280

aguauuaucg acauuaugcg cggagauagc uugaaucuca ucgaaauaau ucgggacaag     2340

aagaucggca uuggggagug gaucgauaac gaaaaucugg acagagagac cggagaaucu     2400

gaugugucca ggcuagaguc aguauucgag gguauaguca ugaguccccg ugugaaacgc     2460

ggcguugugc aggcaguccg ggugaucgac gaucuggcca aggcuauugg gaacccaccu     2520

cagagaaucu uuauugaaau ggccgacgag guacaggcaa gccaaaggac agugucgcga     2580

cucucccggc ugaaagcgcu uuacggaucu cccagcuugc agaaggaguu caaugaucug     2640

aaagacuuac gcaagucacu caacgaaacu aaggaugcug accugcagga agacagacuu     2700

uaucuguacu auauccaguu gggcaaagau cucuacaccg guaaggagau aaguauugac     2760

ucccugucua gcgccuacga uaucgaccac aucguuccac aagcacuaac acaggaugac     2820

uccauugaua auaggguccu ggugucacgg aaagagaacg cccgaaagac ugacagcuau     2880

cucuacaccc ccgaucugau caaaaauaug agaccucuuu gggggaaguu gcuggcugcc     2940

ggcuuaauga gucagaaaaa guaugacgca cucacacgcg uggaugacuu caaggauagg     3000

gaaaaacaac guuuugucca gcggucucug guugagacca gacagauuau gaagaacgug     3060

uccacuauac uuaggacgua cuauggagaa gacacaaacg ugguaacccu gaaaagcucg     3120

aucacucaug acaugcgcaa guaccuaggc uucccauaua aaaaucgaga uauuaacgac     3180

uaccaccaug cucaggaugc ccucuguauc ucagcggccg ggcaauuuau gcugaauaga     3240

cacuucgucu cuggaggcga ggugaccgac ggugcuagua acgcauacaa uauguauuug     3300

gccgaauacc ugcaggaguu ucgggaugcu aaggccaggg gagagcgcaa gcguuccaca     3360

gcauucggau uuguuguggg cagcaugucu agcaaagacg augaaaagag aaccgaccau     3420

cggacuggug agauugucug guccgaaaca aacgcggagu auuuaaggaa agugaugaau     3480

uacaagaaaa ugcucaucac gcagaagguu ggcgauguga agacccgcgc ccuguaugac     3540

gagacucgau acgggcacaa agaaucaagu gcuaagagca uaaaauucga uaaccuuaga     3600

cgggacacau cucuguaugg aggcuuuucc ggggccucaa ccgcauacau gauguuggua     3660

gaguacaaga aaaagaauaa gcucgucagu aucacagcuc aggaagccag ccugcuagca     3720

guggccgauc agaaaaacga cacugugucc gucauggacg ggauucuggc uucucacggc     3780

cuuucgggug cgaagauccu ccugccgaaa auuguuaccg ggcaauugcu ggauuauagc     3840

gacgccuuag ugacgcucca gucagcaucc gagcugaaua ccgcuaggca gauguggcuu     3900

ccccgcaacg auuacaauga caucgauaua auucugaaga guaaagacau cgaagaugcc     3960

gcccauaagu ugggauauga cucugauaca guacacgugc ucagaaggau ccaaaagcug     4020

uucgaggcug ucuuucgggu ggcagagagc caguucccuc uacaucagcu guccaaagac     4080

caacaggaag agauucuucg ugauccacag aacuacggcu uugguucagc cguuaagcuc     4140

cugggcgggu uguuaaaagc gcugcacgcu aaugccgaaa acacuaaccu cgcaggacug     4200

gugaaggucc uucauggcga caaagaguau ucugacgaug ggaaggccaa agaguucaag     4260

aaguucggau uuagcccccg cuggcgaaga auaaguaauc cuaccggcuu cacacugucc     4320

gacagcgaua cuuuuaucua ccagucucca ucagguauuu ucgaacaaag ggugaccguu     4380

aaagaguuga aggaaaaagc ugacaagcag aaauga                               4416


<210>  3393
<211>  4422
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3393
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugggc ccugauagag       60

aaagauacag accagaagaa cgguaauauc uugggcacug gguccagaau uauccccaug      120

ucucaagaua uuaaagacga auuuggaaag ggcaacucaa ucagucagac ggcugauagg      180

accaaauuuc ggagcgugcg ccgauuacgu gagagacauc ugcuaaggcg ggaacgccuc      240

cacagagucc ugaauguucu ugguuuccug ccuaagcacu acgcagacca gauagauuuu      300

aagaaaaggu uggggaagcu cgugaacgag acagaaccaa aacugguaua ugacaauaag      360

aacuucaucu uuaaaaaguc cuucgaggcc augcugucug auuuuaauca gcaucaaccg      420

cucuuacugg cggacggaaa gaaagugccc uucgauugga cuauuuacua ucuucggaag      480

aaagcucuua ccgagaagau cgaaaaagag gaacuggccu ggcuaauucu caacuuuaau      540

cagaagcgcg gcuacuauca gcugcgaggc gaggaggaag aggaaaaccc uaauaaaaug      600

gucgcauucc acucgcuuaa gaucguggag guuauagccg acgaaucaca gaaggggaaa      660

gcugagacau gguacagccu gauuuuggag aacggaugga ucuauagaag gaguuccaag      720

accccacucu acgauuggaa agguaagcaa cgggacuuua uugugacuac agaccugaau      780

gaugacggcu cuaucaaaac cgauaaggaa ggguuagaga agcguagcuu ccgcgcaccc      840

gucgaagacg auuggacgcu ggugaaaaag aaaacugagc aggaaaucaa cagaucccau      900

aagaccguag gaacauacau uuaugagaac cuccuucagg acccuaaaca gaagauaaag      960

ggcaaacugg uuaggacuau cgagcgggaa uuuuacaagg augaguugaa aaugauucua     1020

aagaaucaac agaaauucca cccagaacug aacgacgagc accucuauau caauugcgug     1080

cgagaacugu accgcaacaa ucaggcccau caaaccauac uuucaaagaa ggauuucguc     1140

caccuguuua uugacgauau caucuucuau cagagacccu ugaggaguca gaaaagcucu     1200

auuuccaacu guacacucga guuucggaag uacaaaaaug agaacgguac cgaccagacg     1260

gacuaucuga aggcuauccc uaaaucacau ccauuauucc aagaauuucg ccuguggcag     1320

uggauuuaca aucuagggau auaccagaag gaggaugaca cuaacgugac agaugaacuc     1380

aucaaaaaua uugacggaua ucaggaucug uucgacuuuc uuaacaauaa gaaggagauc     1440

agacaagaua ucuugauuaa auuccuggug gagcagaccg gccucaaggg caaaccgcug     1500

agcagugucg uugccaagua cagguggaac uuuguggaag acaaaauaua ucccugcaau     1560

gagacuggga cacagauccg uacccggcuu gcgaagguag aaaacacgcc ugaggauuuc     1620

cugaccgcag aaacagagau uaaguugugg cacaucauuu acucugugaa cgacaaagcc     1680

gauuaugaga aggcucucaa aacuuuugcc gaaaagcaca aucuggacac ccauuccuuc     1740

gucgagaacu uuaaaaaguu cccacccuuc aagagcgaau acggaucguu uucugagaaa     1800

gcaaucaaga aauuacugcc ucuaauacga cucgguaagu auugguccug ggaaaauauc     1860

gauaaaaaga cacaggagag aauugagaag auccugacug gcgaguucga cgaagcuauu     1920

aaaucaaggg ugcgcgacaa ggccauccac cuuaccgaag aggaucauuu ucaaggguug     1980

cagcuguggc ucgcgaguua cguuguguac ggacggcaca gcgaagccga caaccuggau     2040

aaauggcaua cagucgcaga cuuagaggau uaucuggcug aguucagaca gcacucccuu     2100

aggaauccaa uaguggaaca gguaauuacc gagacuuugc gcguugugaa ggacaucugg     2160

cuccaauacg gccagggugc caaagauuuc uucucugaaa uucacaucga gcuggcucga     2220

gagaugaaga acacggggga cgaacggaaa cgucuaacaa gccuggucac cgauaaugag     2280

aacacuaauc ucagaauaaa ggugcugcuu ucagaauugc ugaacgacgg caaugucgag     2340

aacgugaggc ccuauagucc gaugcagcaa gaagccuuaa agaucuuuga ggauggaguu     2400

cucaauuccg acauugagau cgaagaugac auucugaaaa ucagcaagau ggcacagccu     2460

accaacacag agcuuaaacg cuacaagcug ugguuggaac agaaauaucg gucucccuac     2520

accggccaga uaauuccacu caauagacug uucacugccg acuaugagau cgaacauauc     2580

auuccucaau caagguuuuu cgaugacagu uuuagcaaca agaucauuug ugaguccgcu     2640

gugaaccagc uaaaggauaa acagcugggg cucgaguuca uaaagaauca cucuggaaca     2700

aucguaaaac uggguauggg caagacggcg aaaauucuug acgucgaauc guacaacgag     2760

uuugugaagg ggaauuacac caagaacauc agcaaacgaa auaaguugau gcuggaagag     2820

auucccgaua aaaugaucga acgccaguua aacgacacuc gguauauauc caaguucauc     2880

auuucagcac ucaguaauau cguuagaucu gagagcaacg augacggauu caauuccaaa     2940

aacgugcugc caggcaaugg uaagauuaca ucaaggcuua agcaagauug ggggaugaac     3000

gacaucugga augagcugau acuaccccgu uuugaacgca ugaacaaaau uaccgauagc     3060

gccgucuuca cagcuuggaa ugagaacaac cagaaguuuc ucccuaucgu gccacuggag     3120

uucucuaaag gcuuucagaa gaaacggauc gaccauagac accacgccuu ggacgcacug     3180

guaauugccg cuuccacuag ggaacauaua aauuuacuca acaaugagca cgccaagagu     3240

gauaagcgau ucgaccugaa ccggaaacuu cgcaaguuug aaaaaguggc auacaaugau     3300

ccgaagaccg gaaaaagaau cgagaaggaa guucccacga uuuuccugaa accuugggag     3360

accuuuacag ucgacgcgaa gaacagcuug gaaucuauca uuguguccuu caagcagaau     3420

cucaggguga ucaaccgggc uacuaauuau ucagagaguu acaacgauga gaauggcgcc     3480

cugcgacuag ggaaagacgg aaacccacug aaggagaaaa uaaagcaaag caaaggugau     3540

ucgugggcaa ucagaaagcc caugcauaag gacuuugucu auggcaaagu ucaccuucag     3600

aaugugaagc ucccuaaaga uaaguucauu accgccaucc gcaaaucccu ggacgugaca     3660

uucgauuuga agaggauuga aacuaucacc gacacgggga uacagaagau ucugaaaaac     3720

uacuuacauu cuaaggaaaa uaaaguagag cucgcuuuua gcccagaagg aaucgaggau     3780

augaaccaac acaucgagcg uuauaaugac ggcaagaaac accagcccau ucauaagguc     3840

cggguguucg aaaucgguuc aagauuuagu cugggggagu ccggcaacaa gaaaacaaag     3900

uucguugaag ccgcaaaagg aaccaacuug uuuuucgcug uguaccagga cgauaauaag     3960

ggcaaaagga acuacgagac uauaccgcug aauauuguca ucgaacgcca gaaguugggg     4020

cuccacucug ugccuacaga ggacgccaaa ggaggcaagc ugcuauuuca ucugagccca     4080

aacgauaugg uuuaugugcc caccuccgag gaacuugagg acaaucucgc gucaguaaac     4140

auuaagcgac ugaauacugc ccaaagaaaa cggaucuaca aggucgaaaa agcuagcggu     4200

guggagugcu auuucauaag gcaggauauu gcauacuuga ucaagaacua ugacgccaaa     4260

accaagaucg gggaguuaga aagucagaau aagcugcaga caacgaugac cgaugaccgc     4320

cucaaaauua cugaugugug ugucaagcug aacaucgacc gucuuggauc uauugcugag     4380

acauuucugg gcaaucaaua cggugccucc aaaaagaccu ga                        4422


<210>  3394
<211>  4422
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3394
auggccaaga uccugggccu cgaccuuggg accaacagca uuggaugggc uguggucgag       60

agagaaaaua uagauuucuc ccugaucgac aaagguguua ggaucuuuuc ugagggcgug      120

aagucagaaa aagggauuga gaguagccgg gcagccgaac gcacaggaua ccgauccgcg      180

cguaagauua aauauagaag gaaguugcgg aaguacgaga cuuuaaaagu acugucucua      240

aaccgcaugu gcccccucuc gaucgaggaa guggaggaau ggaagaaauc aggcuucaag      300

gauuauccuc ugaauccaga guuucuuaaa uggcugagca cggacgagga aaguaacguc      360

aauccguacu ucuuuagaga uagggcuucc aagcacaagg ugucuuuguu cgagcucggu      420

cgggccuuuu aucauauagc acagcgccga ggguuccuga gcaacagacu ggaccaaucc      480

gccgaaggaa uccucgagga acacuguccc aaaauugagg cuaucguuga ggauuuaauu      540

ucaaucgacg aaauaaguac caauauuaca gauuacuuuu ucgagacugg cauccuggac      600

agcaacgaaa agaauggcua ugcaaaagau cuugacgagg gggauaagaa auuggugucu      660

cuguacaagu cccuacucgc cauucugaaa aagaacgaau cagacuuuga gaauugcaag      720

agcgagauca ucgaaaggcu uaacaaaaag gacguccugg gaaaagugaa ggguaaaauu      780

aaggauauaa gucaggcuau guuggacggc aauuacaaga cccucgggca guauuucuac      840

ucucuguauu ccaaagagaa gauccggaac caguacacaa gccgugagga acauuauuua      900

ucggaauuua uuaccaucug uaaaguacaa ggaauagauc agauuaauga ggaggaaaag      960

aucaacgaga aaaaguucga cggccuggcc aaggaucucu acaaagcgau cuucuuucag     1020

cgcccucuua agucucagaa aggucugauu gggaagugcu ccuucgaaaa aucaaagagu     1080

agaugugcaa ucagccaccc agacuuugag gaguauagga uguggacuua cuugaacaca     1140

auuaagauag gaacccaauc cgauaaaaag cuacgguucc ugacgcagga cgaaaaacuc     1200

aagcugguuc ccaaauuuua ccgaaagaau gauuucaacu uugacgugcu ugccaaagag     1260

cugaucgaaa agggcucuag cuucggcuuu uauaagucaa guaaaaagaa ugauuucuuu     1320

uacugguuca acuauaaacc uacugacacc gucgcugccu gccagguggc agcuuccuug     1380

aagaaugcca uuggggagga cuggaaaaca aagagcuuua aguaccagac uaucaacucu     1440

aauaaagaac aagugucacg caccgucgau uauaaggacc ucuggcaucu guuaacaguu     1500

gcgaccagug auguguaccu guaugaguuc gccaucgaca aacuaggacu cgaugagaag     1560

aacgcaaagg cuuucagcaa gacgaagcug aaaaaggacu uugccucccu uucuuugucg     1620

gcuauuaaua aaauacugcc auaccucaag gaaggucugc uuuacagcca cgccguauuc     1680

guggcaaaca ucgagaauau ugucgaugaa aacaucugga aagacgagaa gcagagagau     1740

uauauuaaga cucagaucuc cgaaauaauc gagaauuaca cacuggagaa aucaagguuu     1800

gaaauuauca acggcuugcu caaggaguau aaaagugaaa augaggacgg gaagcgggug     1860

uacuauucua aagaagccga gcagagcuuc gagaacgauc ugaagaaaaa guuaguucug     1920

uuuuacaagu ccaacgaaau ugagaauaaa gaacagcagg agaccaucuu caacgagcua     1980

cuccccauau uuauucagca acugaaggac uaugaguuca ucaaaauuca gcgccuugau     2040

cagaaggugu ugaucuuucu gaaaggaaag aaugaaacug gccagauauu cuguacagaa     2100

gagaagggua ccgcugaaga gaaagagaag aaaaucaaga acagacucaa aaagcuguac     2160

cacccuucag acauugaaaa guuuaaaaag aaaaucauua aggacgaguu cgggaaugaa     2220

aagaucgucu uaggcagccc acugacgccg ucuauaaaga accccauggc gaugagggca     2280

cuucaucaau ugcguaaggu gcucaaugcc cugauucuag agggacagau cgaugagaaa     2340

accaucauuc acaucgaaau ggcucgggag cugaacgacg ccaauaagcg aaaaggcauu     2400

caggauuacc agaacgacaa uaagaaauuu agagaagaug caauaaagga gaucaagaaa     2460

cucuauuucg aagacugcaa gaaagaggua gaaccuacag aggaugacau ucugagguac     2520

caacuuugga uggagcagaa ccgcuccgaa aucuaugagg aagggaagaa uauuaguauc     2580

ugugauauaa ucggaagcaa cccagccuac gacauugagc auacuauccc ccggucuaga     2640

ucccaggaua auucacagau gaacaaaacc uugugcaguc aaagguucaa ccgcgagguu     2700

aagaaacaga gcaugccuau ugaacugaau aaccacuuag agauccuccc acgaauagcu     2760

cacuggaagg aagaggccga caaucugaca cgggaaauug agaucaucuc gcguuccauu     2820

aaggcagcgg cuacuaaaga gauaaaggau aaaaagauca gaaggcgcca uuaucuuacc     2880

cugaaacggg acuacuugca ggguaaguau gacagauuua uuugggagga acccaaggug     2940

ggcuucaaaa acucucaaau cccggauaca gggauuauca ccaaguacgc ccaggcauac     3000

cucaaaagcu auuuuaagaa agucgaauca gugaagggag gcauggucgc cgaguucagg     3060

aagauauggg guauccagga aaguuuuauu gacgagaaug ggaugaaaca cuacaaggug     3120

aaagaucgau ccaagcauac ucaccauacg aucgacgcua uuacaaucgc cuguaugacc     3180

aaagagaagu augauguucu ggcacacgcu uggacucuag aagaccagca aaacaagaaa     3240

gaggcccgcu cuauaauuga agcgagcaag ccuuggaaaa ccuucaagga ggaucugcuc     3300

aaaaucgaag aggagauccu ggugucccac uacacacccg acaauguaaa gaaacaggcc     3360

aagaagauug uccgggugag aggcaaaaag caguuuguug cugaagugga gagggauguc     3420

aacggaaaag cagugccaaa gaaagccgcu ucaggcaaga ccaucuauaa acuugacggg     3480

gaagguaaaa aguugccucg ucugcagcaa ggagauacua uacgcggcag cuuacaucag     3540

gacaguauuu acggggccau caaaaauccc cucaacacag acgagauuaa guauguaauc     3600

cggaaagauc uggagucuau aaagggaucc gacguggaaa gcauuguuga ugaggucgug     3660

aaggagaaaa ucaaggaagc aaucgcgaau aaagugcuuc ugcuaucguc aaacgcccag     3720

cagaagaaua aacucgucgg cacgguuugg augaacgaag agaagagaau ugcuaucaau     3780

aaggugagga uuuacgccaa cucugugaaa aacccacugc acauaaagga gcauaguuug     3840

cuguccaaaa gcaagcacgu acacaaacaa aaggucuacg gucagaauga cgaaaacuau     3900

gcaauggcua ucuacgaguu agaugggaaa cgagacuucg aacucauuaa uaucuuuaac     3960

cuggccaaac uuaucaagca gggccaagga uucuaucccc ugcauaaaaa gaaagagauu     4020

aagggcaaaa uaguguuugu uccuaucgaa aagcggaaua agcgcgaugu ggucuugaaa     4080

agagggcaac aggugguuuu cuacgacaag gagguggaga acccaaaaga uauuucugaa     4140

aucguagacu ucaagggaag gauuuauauc auagagggcc ucagcaucca gcggauuguc     4200

cgaccguccg guaaagugga ugaauacggg gugaucaugc ugagauauuu uaaggaggca     4260

cgcaaggccg acgauauuaa acaagacaau uucaagcccg acggagucuu uaaacuaggc     4320

gaaaacaagc cuaccaggaa aaugaaucac aaccaguuca cugcuuuugu ugaggguauc     4380

gauuucaagg ugcugccauc aggcaaguuu gagaaaauau ga                        4422


<210>  3395
<211>  4425
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3395
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugauagag       60

uccgauuucg aaaagaaaca ggguaagauc aauggcuugg ggucuagaau uauccccaug      120

ucacaagaca uuuuaggaaa auuugauagu ggcgugagca ucucccagac agcugagagg      180

acuucuuacc gggguguccg ccgacugaac cagcguaauc uacucagaag ggaacggcug      240

caccgcguuc uuaacauacu ggacuucuug ccuaagcauu auucgaaugc aaucgauuuu      300

gagaagaaac ucgggcaguu caaggaaaac guggaggaga aacugaauua cgccaagaac      360

caaauuggaa gacacgaauu uaucuucaug gacucauuua aagagauggu aaaggaguuc      420

aagcagacgc agccacagcu guuuuauaaa agggaaaaug gcauugagac caagaucccg      480

uacgauugga cacucuauua cuuacggaaa aaggcgcuga gccaaaagau aaagaaagag      540

gaacuugcuu ggauuaucuu gaacuucaau cagaagcgcg gcuauuacca gcugcgaggg      600

gaagacgugc uagaugagag uaagauugaa gacuuuuacu cccucaaagu cguggagguu      660

caggagacug aagauaagaa cgccagagga accugguaua augugauccu ggagaacgac      720

ugggucuaca aaaggcaauc uaaggaaagc cuugauaauu ggaucgguaa aaacaaggag      780

uucauuguga caaccaacaa ggaaaaagac ggcucccuga aguuggacaa agcagggaau      840

guaaagcgga acuuucgugc cguugauuca gagaaagacu ggauagcuau caagaagaaa      900

acugagcagg auguggaaau uaguaagcau acagucggac aguauaucua cgagacccuc      960

cugcaaaaug caaaacagaa gauaaaaggc aaguuaguga agacgauuga acgcaaauuc     1020

uauaaggagg aguucaucaa aaucauuaag gaacagcuga aacaccaucc cgagcuccag     1080

agcaagacuc uuuacgaaac cugcauccaa gagcuguauc cuagaaacga agcccaccag     1140

aauaacauua aaucuaaggg uuuugaguac uuguucacag acgauauaau cuuuuaccag     1200

aggccacuaa aguccaaaaa gucaacuauu agcggguguc aguaugagac ccggcuguuc     1260

aaaaagacag ugcucaauaa agucaccaac aaggaaaaug agaucuggca aaaggaaccc     1320

cugaaagcua ucaguaaguc ucacccucuu uuucaggagu uccgacugug gcaguuuuug     1380

aaaaaccuca agauuuacca gaaagaagac guuaagggaa aucugauaga ucaugacauc     1440

acguccgauu uacugaagac ugaggccgac uggguggagc uauucgauuu ucucaacauu     1500

aaaaaggaaa ucgagcaaaa acagauuauc caguucuuua uaaauaacaa gcagaucgaa     1560

aaaggcaaga agaauaacua ucgcuggaac uacguagagg acaaaaagua uccaugcaau     1620

gaaacaaaaa gccaacugau uucgagacuu aagaaaguga agaacuugga ccugaaucuc     1680

uucuuuaccu cugagacuga gcugaaacuu uggcacauca uuuacuccgu caaggauaag     1740

auccaguaug aaaaagcgau aaagaacuuc gcaauuaaaa auaacaucga cauugauuca     1800

uucguggaga auuuuaagag guuucccccu uuuaaaaacg aauacgccgc uuacagugag     1860

aaggccauca agcggcuguu gccacucaug cgcaugggcg aauauuggaa ugagagcgag     1920

auaaaacagg aaguuaagga cagagcauac uccaucauug agaggcugaa agggaucgau     1980

uucaacaagg acaaaauuga ugaaaagauc acagacgaug acauaaagaa acaauuacug     2040

aagucuuuua uugauuucaa agacaagaau ccgcuaaaag gacucaacac cuaucaggcu     2100

uguuaccugg uguauaaucg ucauagcgag gucaacucaa ucgccuacug gaagaguccc     2160

gaugacauca auaaguaucu ugaggguuuu aaacagcacu ccuugcggaa cccuauugug     2220

gaacagguag uuacggagac ccugcgaaca gugagagaca ucuggcaaua cuacggcaag     2280

gggaaagcga gcuucuuuaa cgaaauucac gucgagcucg gaagggaaau gaagaaucca     2340

gccuauaaac gcaaggcauc uacuaaccgg gugaccgaga augagaacac aaaucauaga     2400

auaaaggaaa uccugaaaga guuacagaac gaaggcgucc aggaggugaa gucauacagu     2460

aggaaucagc aagaaauucu gaaaaucuau gaggagggua uuuaccagag caucgauguu     2520

guggacgaug cuauagaaaa gauccgcaaa aacuccucuc ccacuaaggc cgagauuauc     2580

aaauauaaac uuugguugga acagggguac accucgccuu auacaggcaa gaccauucca     2640

augagcaaac ucuucacuac ggacuaccag aucgagcaca uaauucccca aucccuguac     2700

uuugauaauu cacuaaguaa caagaucauc ugcgaaucug agguaaauca gcugaaagac     2760

aaccagacag cuuaugaguu cauuaagaaa cauggaaccg aaaaggucag ccucggcaag     2820

aauaaaacug uggagauauu uacccuggaa caauacgaga agcacuguaa ccaguauuuc     2880

aaaaagaaua aaaacaaacu uaagaaauug cuguccgagg aaaucccgga gggguucauu     2940

aaccgacagu uaaaugauuc acgguacauc agcaaguuua uuaaaggacu ccuggguaac     3000

cuuguucgug aagaggacga acaggaggug acaaccaagc auaucguccc ugugacuggc     3060

gccauaacau cuaaacugaa gcaagauugg ggguugaaug acaaguggaa cgagcucgua     3120

gcauccagau uccagaggcu gaauacgaug accaacagua auaacuuugg auauugggau     3180

acuaaaauca augccuuccg cgugcagguu cccgacgaaa uuagcaaggg cuuuucuaaa     3240

aagcggaucg aucacagaca ccaugcucua gacgcgcugg ucauugcaug cuguacaagg     3300

aaccacauca auuaccucaa cgcccugaau gcugagaaaa agaacuaugg ucuucgagac     3360

uugcuguuaa uaaagaauga uaagaaccau uacaccaagu ucuuuaaaca cccaugggac     3420

ggguucccug uggaagccaa guccgugcuc gagaaaacaa uuaucucauu uaagcagaau     3480

acucgcguca ucaacaaaac caacaauaag acguggcaau gggcagaaaa ggagggccag     3540

cugaaaaagg uucuugugaa acagaccaag ggagauaacu gggccauucg gaaacccaug     3600

cacaaggaga cagugagugg caagcugaau aucccaacuc ccaaagggaa gauagcuacc     3660

gccagcagag uaucgcuauc cgaaauuaaa acaaggaagc agcucgacaa aaucacugau     3720

auuucuaucc aaaagauacu ggagcgucau uugaacaauu accuggacga uaagggaaaa     3780

gaaaaguucg acuuagcauu uaacgcggau ggugucgagg aacucaauaa aaacauuaag     3840

gugcugaaua aaggcaagaa ccaccagccu aucuauaaag uucgccuuua cgagaucggg     3900

agccgguuca aggugggaaa uucaggcaac aagaaagaca aguaugucga ggcugccaaa     3960

gguaccaauc uguucuuugc aauuuacuug aacgaaaagg auaaaaagag aguguaugag     4020

acgaucccac ucaaugaagu uauugagcau cagaaacagg ugaguaaccu gccgaagaac     4080

gagaggacac ccguaccugu caaucacacc aaggggaaau uccuauuuuc ccugucucca     4140

aacgaccuug uguacgugcc cacugacgaa gagauagaua auccuagccu cgucaacuuc     4200

aauaaccuga auaagaaaca aguugaaaag aucuauaaga caguguccug cucaaccuac     4260

caguguuucu ucguacauaa cacuauuagc aaaccaaucc agaauaaggu ggaguuuagu     4320

gccuugaaca aaauggaaaa gucuaucgac ggcauuauga uaaaagaucg augcuggaag     4380

cugaagaugg accgguuagg aaauaucauu uccgucaucc gcuga                     4425


<210>  3396
<211>  4443
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3396
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc caaagugugc       60

gucgauaaug acgguaacua ccugagagau auaaaguugg gcuccaggau cauucccaug      120

ucucaggaca cuuuagggaa uuucgauaaa ggaguuacgg acucaccuac cgcugcacgg      180

acaggcuuuc gcggugugcg acguaucaga gagagggccc ugcaacggcg cgaaagacua      240

cacaggguac uccaugugau gaaguuccug ccaccgcacu augagaacgc gauugggugg      300

aaucgggaag aggcuacuac cuacggaaaa uuucuuaacc auggcgaacc caagcuggcc      360

ugggagaagc aggaggaugg caguaugcgc uucuuguuua uggacagcuu ccacgaaaug      420

auggcagauu uugccgaguc ccagccuggg cucgucgcug gagguaaaaa gaucccacug      480

gacuggacac uguauuaccu ccgaaagaaa gcauuaaccc aggccauaag aaaagaggaa      540

cuggcuugga uccuuuugaa uuucaaccaa aagaggggcu auuaccagcu gagaggggag      600

gaagaugagg aaaaucccgc caagcgugag gaauaucaug agcuaaaagu gguuucugug      660

gaggcggcag aagagggaaa gggcgguaac acuugguaca augucacacu cgaaaacggg      720

uggauuuacc gcagacaguc gaaaguggag cugcacgacu ggguaggaaa gaccaggcag      780

uuuaucguua cgacugaaua ugagaaagau ggcgugacca uuaagaaaga caagaauggc      840

gaggucaagc ggucauuccg agccccuagc gaagacgauu gggggcuucg caaaaagaga      900

acagagaguc uguuggaaga cuccggaaaa acugugggug cuuuuaucua cgaucacaua      960

cucucugagc caaccgacaa gauuaggggc aacuucaucc ggacaauuga acgcaaauau     1020

uacaagaagg agcuggaggc caucuuacug gaacaaacca aacaucacaa ggagcucaaa     1080

gaucagaaua cgcuuaucgc auguauuaag gaacuguauc agagcaacaa accccaucag     1140

gacuccuuga ugaagaagga uaugacuuac cuacugauaa cagaccucau cuuuuaucaa     1200

agaccucuga aaucaaagaa aagucuuauu gcugauugcc cauacgagua cuaugaguac     1260

accaucaagg aaacugggga gauaaaaagg cagggaauua aguguaucgc caagagcaac     1320

ccguauuacc aggaauuucg ucuguggcaa uucaucauua auuugcggcu cuuugaccga     1380

acagaugaca aagaggugac cucugaauau cuguccucaa uggaggacua cacgaaguua     1440

uucaccuauc ugaacgauag aaaagacauc aaucaggaga cacuacucaa ggauuucuuc     1500

aaacagaaga aagucaagau gggcauggaa aaggaguuuc ccauuaggug gaacuacaua     1560

gaagacaaag agaagaaaua cccuugcaau gaaacucgcc acgagcugcu ugcggccuug     1620

gauaaggcag guaucgacca cagcuggcug gaugacaaag agcgguucua uagacucugg     1680

caucugcuuu acaguguuga aucuaaggag gaagcugagg gggcccugag gaaguugaaa     1740

gauaacgacg auuuugugaa ugcuuuccuc aagguaaaac cauuuaagaa agacuauggc     1800

gccuacuccg aaaaggcagu gaagaaacug uuagccauua ugcgcauggg aggccugugg     1860

aacgagaccg auaucuguga gcagacacga aaucggaucc aaagcauuau aaagggggaa     1920

aucgacgaga aaauuaagga acguauggga agaacuuucu gccagcuauc ggacuuucag     1980

ggucuccccg aguggcuggc uuguuauguc guguacggca ggcacucuga ggcgaccgau     2040

auccagcgcu ggacaaaacc ugaaaaccuu auggcauaua uuaauggguu caagcaacau     2100

uccuugcgga acccaaucgu ugagcagugc auacuggaaa cccucagaac ugugcacgac     2160

aucuggaagg aggccggaca uauugaugaa auccacgucg agcugggcag gucaaugaaa     2220

aguacggcug accagcgagc ccgcaugagc aaguccauuu uacagaauga gaacacaaau     2280

cugcggauca aaucucuuuu gauggaacuc aagaacgcaa aagagauaga aaacgugaga     2340

cccuacagcc cgaugcaaca ggagauucug aggaucuacg aagagggugc ccuacaggag     2400

cugaccaagg aagaugcuca guauccugag auuucaaaaa ucagucaaau ggcccagccc     2460

uccgcaaagg aacuccugaa guaccgucuu ugguuggagc agaaauauug uagcccauac     2520

acugggaagc cuauaucucu gucaaaauua uuuaccagug cguaucaaau cgaacacauu     2580

aucccccaga gccgcuacuu cgacgauucc uucacaaaua agguaauuug cgagaccgag     2640

guuaaccagc ucaaaucuaa uaugcugggc uaugaauuua ucaagaagca uucgggagag     2700

auagugcacu guacuacaau uggcgacguc aaaauccuug augcuaagga auacuacaac     2760

uucgugacgg agcauuaugc caauaaccgg cggaaaaagg agaaacuguu gcucgaagac     2820

aucccacagg aguuucugaa uaggcaaaug aacgauagcc gauacauuuc caagaagguc     2880

auggcaaucc uaucaaauau ugugcgggaa gaggacgaac aggagaccaa caguaaauuc     2940

guugugucuu gcagcggggg aauaacugau cgccugaagc aggacugggg ucucaaugac     3000

guauggaacu ccaucgucua ucccagauuu gagaggcuga auaaacuuac acagaccgaa     3060

gccuucggcc ggugggagaa caaggaaggg aaacaagugu uucagacauc auugccucug     3120

gaguuacagc gaggauucag caagaagaga auugaucacc gccaccaugc uauggacgcc     3180

cucguuaucg caugcgcuuc uaggaauauu aucaacuacc ugaacaauga auccgccaaa     3240

agucaggagc aacgugagga ucuucggaag aaacugugug acaagaacag aauaaucagg     3300

aaaccauggg aaacuuuuac ccaggaugcg cgcacggccc uagaggacgu ggucgugagc     3360

uucaagaauu auguacgagu gauuaacaag gcuaccaauu acuaugaaaa auacgaugag     3420

ucuggcaaga aagguauggc agaacagaag gggcaagaca ugugggccau cagaaaaccg     3480

augcacaagg agacaguuuu uggccagguc aaccuccgga ggaaagugac ugugaagcug     3540

aaggaugcuu uggagaauau ucccgccauc ugcgacaaag cacugcgcga uuacgucaac     3600

gacuuaauag cgaagaaauu caauaccaag cagaugcucg cccauuuuaa auccauuaac     3660

uaucgucuga auaugaaguc aguuaacaag gugggaaucu ggguguucag ugacgaaaaa     3720

gagccuaugg uagcuacaag aaaggccauc gauauuagcu ucgacgcaaa acggauagau     3780

acuaucaccg acacgggcau ucagcaaauc cuuuguaagu accugaaauc gaaggggaau     3840

gauccaaacg ucgcuuuuac acccgaagga auugccgaga ugaauaagca caucauagac     3900

uauaacaacg gcaaacagca ucagccuauc uugaagguga ggguuuccga gccaaaaggu     3960

gcaaaguacc agguggggga aaccggaaau aaaacuaaga aguucgucga ggcccaaaaa     4020

ggcacaaacc ucuauuuugc uauuuacgaa gauaaggacg guaaucgcuc uuauaacacc     4080

aucccccuga augagguggc ggaacgacua aaacagggcc ugagcccggu uccugagaag     4140

aacgagaaag ggauuucacu uaaguucuac cucaguccaa augaucuggu guacguaccc     4200

acugaagagg aaaaauuguc caacgagugc aagcugucua aggaaagaau cuauaaaaug     4260

gucagcucca ccucaaauga gugucacuuu auaccuuaca caacggccaa gcccauuuau     4320

gacaaauucg aguacgaagc aaagaacaaa augggacggg cuuuaaccgg cgagaugauc     4380

aaggccgugu gcuggaagcu cgaaguggau aggcugggga auauccuuaa aauuaucaag     4440

uga                                                                   4443


<210>  3397
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3397
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc caaaauaucc       60

aucgaugaca augguaacua ccugagagau auucagaugg gcucuaggau cauucccaug      120

ucacaagacg uguuggggaa uuucgauagu ggagucacug agagccagac ggcuguucgg      180

accggcuuuc gcgguuuacg acguaucaga gaaagguccc ugcuacggcg cgagagacuc      240

cacagggugc ugcauguaau gggguuccuu ccuccacacu augacucugc aauaggaugg      300

aacaaggagg aaccgacaac uuacggcaaa uuucugaauc auggcgaacc caaguuggcc      360

ugggagcggc agggggaugg aucgaugcgc uuccucuuua uggacucauu ccacaaaaug      420

auggaggauu uugcgccuca ucagccagcu cugguggccg acggucgaaa gaucccccug      480

gauuggaccc ucuauuacuu aagaaagaaa gcacugacac aaccuauuaa gaaagaggaa      540

cuugccugga ucuugcugaa cuucaaucag aagaggggcu auuaccagcu acguggggaa      600

gaggaggaag agaacccaac caaacgggaa gaguaucacg aacucaaggu cgugagcguu      660

gaggcugcag aggaaggaaa gggcgguaau acuugguaca acgugaaacu ggagaauggg      720

uggauuuacc gcagaaguuc caagaucccc cuugacgauu gggucggaaa aacaaggccu      780

uuuauaguga ccacggaaua ugaggccgac ggcauuacuc ugaagaaaaa caaggaaggc      840

gagguaaaac ggucuuuccg agcuccaggg gaggacgauu ggggauugcg caagaagaga      900

accgaaagcc agcucgaggc cuccgguaaa acaguuggcg cguuuaucua cgaccaccug      960

uuagcagaac cggccgauaa gauuaggggg aauuucaucc ggacuaucga gcgcaaauau     1020

uacaagcaag aacuggcuga cauucucaga gagcagucaa aauaucauga ggaacuucag     1080

aguaagaaga uacuggccga uugcgcagag gaauuguaca ggagcaacca gccccaccgu     1140

gacucucuac ugaaaaagga uaugguguau cuccugcuua ccgacaucau uuucuaccaa     1200

cggccucuga aauccaagaa aucauugauc gcugauuguc cauacgagua uuacgaguau     1260

gucgacaagg aaacaggaga gauaaagcga cagggcauua aaugcguggc caagagcaau     1320

cccuacuauc aggaauuuag acucuggcaa uucgugcuga acuuaaggcu guacgaucgc     1380

accgaggaca aagaagucac ggcggaguau cuaccuagua aggaggacua cgcccggcuc     1440

uuuguuuacc ugaacgauag aaaagaaauc aaucaggaga cucuuuugaa ggacuucuuu     1500

aagcugaaaa aggugaaaca gaggucugaa aaggaguucc caguacgcug gaacuauauc     1560

gaagauaaag agaagaaaua ccccuguaau gagacacgac augaacuccu gauggcacuu     1620

gaccgggcug guauugagcg uuccuggcug gaugacaccg ccagacagug cagguugugg     1680

caccuccugu auagcaucga agugaaggau gaggcugaac gcgccuuacg gaagcugaaa     1740

gacgaugacg aguuugucuc ggcauuccua aaggugaaac cguuuaagaa agauuacggg     1800

gccuauucug agaaggcugu uaagaaacuc cuggcgguga ugagaauggg cucccacugg     1860

ugugaagacg auauuugcaa ggagacuaaa gaaaggauag agcauauccu ucaaggagac     1920

gucgaggaaa agauuaaaga gcgaaugaac cacacagcau acucauuggg cgaacugagu     1980

gacuuccagg cccucccuga guggcuggcu uguuaugugg uauacgggcg ccauagcgaa     2040

gccaccgaua ucaagcacug gaagacgccc gaguccuuaa uggcauacau caauggauuu     2100

aaacagcauu cucugcggaa cccaauuguu gagcagugca uacuugaaac cuugagaaca     2160

gugcacgaca ucuggaagga ggccggucac auugaugaaa uccaugucga gcucggcagg     2220

agcaugaaau caaccaguga ccaacgugcu cgcaugacug aaaauauucu gcggaacgag     2280

aauacaaacc uaagaaucaa gucccugcuc auggagcuga aaaaugaugc cgacauaaag     2340

gaugugaggc cuuauagccc caugcagcag gaaauccuuc gaauuuacga ggaaggggca     2400

uugcaggagc ugacuaagga agacaacgau uaugaggaga ucuucaaaau uucuaagaug     2460

ccacaacccu cagcgaaaga auuacucaag uacaaacugu ggcuugagca gaaguaucgg     2520

aguccuuaca ccggaaaacc aaucagccug uccaaguugu uuacaucugc uuaucagaua     2580

gaacacauua ucccgcaguc gcgcuacuuc gacgauagcu uuaauaacaa ggucauuugu     2640

gaguccgagg ugaaugcccu caaaggcaac augcuggguu acgaguucau caagaaccau     2700

gggggcgaaa uaguucacug cacccuacug ggagacguga aaguacaaaa ugaaucagag     2760

uauaaggcau ucgucacuga acacuacgcc aacaauaaau auaagaagga gaaacuccug     2820

auggaggaua ucccccagga cuuucuuaac agacauuuga augacaguag guacauuucu     2880

aaggugguua aaacgcuguu aagcaacauc gugcggacag aagaugagau ggaaaccacu     2940

uccaaguucg ucgugucaug uaccggcggg auuacagacc gacucaaaaa ggauugggga     3000

cugaaugacg uauggaacag caucguguau ucuagauuug agcgccuuaa ugaacugacc     3060

aaguccgaug cuuucgguag gugggagaac aaagagggca agcguguuuu ucagacaagu     3120

gucccucuag aacuccaacg gggguucaau aaaaagagaa uagaccacag gcaucacgcc     3180

auggaugcac uggugauugc uugcgccagc cgcaacauca ucaauuacuu gaacaaugag     3240

ucugcgaacu ccccaaagaa gcgagaagac cugagacagc aguuaugcga uaagaaccgg     3300

auuaucagga aacccuggga gucauuuacu caggacgccc gcgcugcacu cgaagaugug     3360

gucguuaguu ucaagaauua ugugcgugug auuaacaaag ccacgaauua cuacgagaga     3420

uaugacgcug acggaaagaa aaccauaacu aagcaaaaga gccgggagau gugggccgua     3480

aggaaaccua ugcauaagga aacagucuuu ggccacguga accugcgccg aaaagcaauc     3540

guuaaacuua aagaugcgcu ggagaauauu ccaucgaucu guaacaagga auugagagac     3600

uacauuaaug gucucgugga gcggaacuuc aauaccaaac agcugcacgc ccauuuuagg     3660

uccacagauu aucgcuggaa caagcagaau gucgacaagg uggagguuug gagauucucu     3720

gaugaaaaag agccccuagu ggcuacuagg aagccgcugg acaccagcuu ugaugccaaa     3780

cggaucgaca cgauaaccga uacagggauc cagaagauuc uucucaacua ccuggaagca     3840

aaaggcggag acccuuugau cgcuuucacu ccagagggca uugccgaaau gaaucaaaac     3900

aucgcacugu auaaugaugg gaagaugcac cagcccauau uaaagguacg uauuucagag     3960

ccuaaaggag ccaaguacca gaucggccag accgguaaca aagcugacaa guucgucgag     4020

gcgcaaaaag ggacaaaccu cuauuuugcc aucuacgaag augaggaccg caaucgaacu     4080

uacaacacca uuccccugaa ugaaguggca gagagacuua agcagggacu gccaccugug     4140

cccgaaaaga acgagaaaga ggucccauug aaguucuauc ucaguccgaa ugaccugguu     4200

uacgugccua cggaagagga aauacuaaug aaagagugca agcugaacaa aaauaggauc     4260

uauaagaugg uauccucuac aggcaaccag ugcuuuuuca uucccuuucg gguggcuguc     4320

agcaucguga auaagaugga auacucccca cuuaacaaaa uggagaaggc cauuucaagc     4380

gagaugauca aagccguuug uuggaagcuc gaaguggauc gccuggguaa uauaaucaga     4440

gucgugaaau ga                                                         4452


<210>  3398
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3398
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc caaagugucc       60

cuggaugaca augguaacua cuugcacgau auaaaguuag gcugcagaau cauucccaug      120

ucucaggacg uccuggauaa uuucgggaaa ggaguuacug ugucacaaac gaguguaagg      180

accggcuuuc ggggugugcg ccgaauccgu gagagaagcc uacagaggcg ggaacgccuc      240

cauagagucc ugcacaggcu uggguuccug ccucagcauu augacgcugc aauuggaugg      300

gauuccgacu gugccaagac auacggcaaa uuuuuggaua acggcgagcc aaagcucgcg      360

ugggaacggc aggaggacgg gucuaugcgc uuccuguuua ugaacucguu cuaugaaaug      420

aucgcugauu uuacuucaca ccaaccggcc cugguggcag acggaaagaa aauaccccuc      480

gauuggaccu uauacuaucu gcgaaagaaa gcccuuacac aggcuaucag aaaggaggag      540

uuggcaugga uucugcuuag cuucaaucag aaaagggguu acuaucagcu ccggggcgaa      600

gaggaagacg agaauccuac caagcgugag gaauuuugcg agcugaaggu uguggccguc      660

gaagcugccg agaaagggaa gggagacgau aucugguaca acgugcaucu ugaaaauggc      720

uguauuuacc gcagaaguuc caaaaucucu cuggacgauu ggguagguaa gacuaaacaa      780

uucauaauua caaccgagua ugagaaggac gggguuacgc ccaaaaagga uaaggacgga      840

aaagaaaaga ggagcuaccg ggcgccaaaa gaggaugacu ggggcuugcg aaagaaacgc      900

acugaaaccg aucucgaggc auccggcaag acaguggggg ccuuuaucua ugaacaccug      960

uuagcugagc cuuucgacaa gauuagagga aacuuuaucc acacuaucga gaggaaauac     1020

uaucgggccg aacuggaggc aauucucacc gaacagcgca aguaccauga ggaacuuaga     1080

ucagagcaga ugcuggcugu cugcgccgag gaauuguaua ggaguaaucu accacaccag     1140

caaagccugc ucaaaaagga uaugguguac cugcuucugu cugacuugau auucuaccag     1200

cguccccuca aauccaagaa gucacugauc gcggacuguc cuuaugagua cuaucgguac     1260

guggauaaag aaacagguga gauuaagaaa cagggcauca agugcauagc caaaagcaac     1320

ccauauuacc aagaguuccg auuauggcag uuuguccuga aucuaagacu cuucgacagg     1380

augaccgaua aggaaguuac ggcagacuau cugccgacua aggaggauua cgugcgccuu     1440

uuugacuacu ugaacaaucg gaaagaaauu aaccaggaga cacugcucaa ggauuucuuu     1500

agacugaaaa agguaaaacu ugggagggaa aaggaguucg agauccgcug gaacuauauc     1560

gaagacaaag agaagaagua ccccuguaau gaaacccgau augagcuguu gguggcucuc     1620

gaucgggccg gaauugaccg uaguuggauc gaugaagacg gcagacagua caggcugugg     1680

cauuuacugu auucuauuga ggucaaagcu gaggccgaau ccgcacuacg caagcucaac     1740

agcgaugacg ccuuuguguc gucuuuccug aaaguuaagc cuuuuaaaaa ggacuacggu     1800

gcuuauuccg agaaggcggu gaaaaagcua uuggcaguca ugcggauggg gucaaugugg     1860

agugaagcca auauaugcga ggaaacucaa aacagaauca ggagcauuau ccgaggcgau     1920

aucgaggaga aaauuaagga acgcauggga cacacagcuc gguccuucaa ucagauagac     1980

gauuuucagg cccugccaga guggcucgca uguuacgugg uauacggcag acacucugaa     2040

guuaccgaca uccagcauug ggagacgccc gagcaacugc agagcuauau uaacggguuc     2100

aaacagcacu cauuaaggaa uccuaucgug gaacagugca uucuggagac ccuucguaca     2160

guccaugaua ucuggaagga agccggacac auagacgaga uccaugugga auugggucgc     2220

gauaugaagc ggacugcuga ccaaagagcc aggaugacca aaaacauucu caagaaugag     2280

aacacaaauc ugcgaaucaa aagucuacug auggagcuca agaaagauac ugacauuaag     2340

gaaguccggc cauacucccc caugcagcag gagauccugc gcauauauga agagggcgca     2400

cuuuugcagc ugaagagaga ugacgaagcg uuugcugaua uuagcaaaau cucucaaaug     2460

gcccagccga ccgcaacaga guuaaggcgg uacaagcucu ggcuggacca gaaauaucga     2520

ucaccuuaca ccgggaaguu cauuagucuu agcaaacugu ucacuuccgc cuaucaaauc     2580

gagcacguga uaccccaguc uagauacuuu gacgauucgu ucagcaacaa gguuaucugu     2640

gaagcugagg ugaaucgcuu gaaauccaac augcucggau acgaauuuau uaaggcccac     2700

ggcggugaga ucguacauug cacgaugcug ggggacguca agauucuauc agaaagugag     2760

uauaaagcau ucgugaauga gcacuacguu aaccauaggg cuaagaaaga aaagcugcuc     2820

auggaggaua ucccacagga auuucugaac cgucagauga augacucucg guauauaagc     2880

aaagugguca aggcccuuuu guccaacauu gugagaacag aggaugagau ggacguaacc     2940

ucaaaguucg ugguuccuug cagcggcgga aucacugaua ggcugaaaaa ggacuggggc     3000

uuaaaugaug ucuggaacuc uaucguguac ccccgcuuug aacgacucaa uagaaugacc     3060

gggacagagg cguucggaca cugggaaaac aaagagggua agcggguguu ucaaaccucc     3120

gucccaauug aacugcagcu uggcuucagu aaaaagagga ucgaccaccg ccaucacgcc     3180

auggaugcuc ugguuauugc augugccagc cguaauauaa ucaacuaucu aaauaacgag     3240

ucugcuaauu cccagaagaa aagagaggac cuccggaggc uguugugcga caagaaccgc     3300

auuauccgaa aacccuggga aucauuuacu caggaugcca gaguggcacu gcaagacauu     3360

gugguaaguu ucaagaauua cguccggauc auaaacaaag cgacaaauua uuacgagagg     3420

uacgaugaac aggggaagaa gacgaccgcc gagcagaaaa gcucggaaau gugggcuauc     3480

cgcaagccua ugcauaaaga gacuguguuu ggacacguua acuuaaagag aaaaacaacc     3540

gugaagcuca agaacgccau ugagaauauc ccaaacauuu guaauaaaga acugaaggca     3600

uauaucaacg agcuugucga aaaacauuuc aauacaaagc agaugcuggc ucacuucaaa     3660

uccauaaacu acagguggaa ucggcaaaac guggagaagg uugaagugug gcaguauucu     3720

gacgagaaag agcguauggc cgcaacucgc aagccguugg auaccagcuu ugacaagaaa     3780

cgaauugaaa cgaucaccga uacaggcauc cagaagauuc uccugaauua ccuagaggaa     3840

aaaggugggg accccaacau agccuucacu ccugagggca ucgcugagau gaauaagaac     3900

auuucacugu uuaaugaugg aaaacuucac caaccaaucc ucaagguaag agucgcgaag     3960

accaugggcg ccaaauauca gguggggcag acaggaaaca aggcagcuaa auacgugggc     4020

gcccaggaag guacuaaucu guauuucgcc auuuacgaga acgaagaggg ggaaaggagu     4080

uacaacgcug uccccuugaa ugagguugca gagcggcuga agcaaggaau guccccugug     4140

ccagagaaaa acgaaaagga cguacccuua aaguuuuauc ucucuccgaa ugaucuggug     4200

uacgucccua ccgaagagga caaacuuagc aacgaaugca agcuggauaa ugagcgcauc     4260

uauaaaaugg uguccgccac gggcaagaaa ugucauuucu ugccauacuc aguugcgaac     4320

auaaucuaua auaaggugga guuugaaagc augaacaaga uggguaaagc ucucaguggc     4380

gagaugauua aggccgucug cuggaaacug gaaguggaca gacuagggac aauccugagg     4440

guuauuaagu ga                                                         4452


<210>  3399
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3399
augaagaccg agcuggaccu caacaaagau agacuuuaca agcacgugcu gggcuuggac       60

uuagggguca caagcaucgg auucgcccau guuguggaau ccgagaauag gaacgaaucu      120

gagauuaaac agauaggugu acgggugauc caacuggaua auuuugacaa ggucgauaaa      180

ucaggcaaga cuuccgaaag caagaacccc gcuguggacu ucgcagccgg gaaaggacua      240

acgccuaaug gcucccgcac caagaaacga ggugcgcgua gaaaccucga uagguaucag      300

gaccggcgcg auaaucugcu ugagguucug acaaagucug gguuuauuaa cucggagacu      360

aaaagaaccg aagacggaaa gcacacaacc caucagacuu gggagaucag ggcuaaggcc      420

gugucagaaa aagucgagaa ggaggaguuc gcacggauuu ugcucgccau caauaaaaag      480

cgcggcuaca aaagcagucg aaaggcuaaa aacgaagaug aaggccaggc aguggacggg      540

auggccguag cuaagagacu guaugaggaa aaucugacac caggacaaua cgccuaugag      600

guucucuccg guggcaagaa aucuauaccg gauuuuuaca ggagcgacuu acaggcggag      660

uucgacaaga ucuggaacuu ucagaaacag uucuauccca auauucugac cgaugaauuu      720

uacgaggaac uuaaggggaa aggacaacgg gcaacguccg ccgcuuucug gaagauccac      780

caguuuaaca cugccgacau uaaggauuug gaggacgaac ugaaaaauga gcguaccauc      840

aagcuaucaa aacgcgauca gaagaaacuc caggcauaca aguggagaag ugaggcugug      900

gucaagcaac ugggcaaaga agagguggcc uaugugcuua cagaaauaaa caacaaucug      960

aacaauagcu cugguuacuu gggggcgauu uccgacaggu caaaggagcu cuauuucaac     1020

aaacagacug ucggacaguu ucuguacaag caauuaaaag aaaauccuca uacccggcug     1080

aagaaccagg uuuucuaucg acaggauuac cucgacgagu ucgagacaau cugggaaaug     1140

caggcccaau uucacccaga gcuuacccag gaacugaagg aggagauucg cgauaucauc     1200

auuuucuauc agagaaaacu aaagagccag aaaggccuaa uaaguuuuug cgaacuggag     1260

ucuaaggaag ugguaaucaa ugacacgaaa aagauuaucg gccucagggu ggcacccaaa     1320

uccagcccuc uguuccaaga guuuaagaua uggcagaacc uuaauaacgu ccuggugcgg     1380

aagaaagggu cgcgcaagag aaugguuaaa gcucagaauc agcacucuuu guucgaggaa     1440

ccaaaggaga uuuuuagucu cgaucuggcc ucaaaagaaa aguuauucga ggaacugaac     1500

cuaaagggaa aucucagugc uaaaaacugu cuggacaucc uugguuugaa uagcaacgag     1560

ugggaacuga acuacacuca acucgagggc aauaggacaa acaagucccu guacgaggcc     1620

uuucuuaaaa uccuggacau ugaaggguau gaugugaagg acuugcucaa agucaagucu     1680

agcaaggaug agguggaacu ggacgauuua aaaguacccg caucagagau caagaauaug     1740

guucgugaaa uuuucaguac caacaaaaua gacacugaga uccuggauuu ugacgccgag     1800

auugauggaa aggcuuucga aaaacagucc agcuaccagc uauggcaucu ccuguauucu     1860

uacgagggcg acgauucaca gagugguaau gaaaaacuuu augaguugcu ggcgaagaaa     1920

uuuggguuca agcgggagca cgcacaaauc cucgccaacg ugagccuggu cgacgauuac     1980

ggcaauuuau ccucuaaagc uaugcgaaag aucuauccuc auauuagaga aaacaauuac     2040

ucgacagccu gcgagcuggu gggauacagg cacagcaaac acucccuuac caaggacgaa     2100

uugaaagacc gcguccucga ggaucggcug gaacuacuga agaagaacuc acucagacag     2160

ccagugguug agaaaauacu gaaucagaug aucaacgcag ugaaugcccu uauugagaag     2220

aacagugaaa gggacgagaa uggcaacauc acgaaauauu ucaaguuuga ugaaauucgc     2280

aucgaguugg cucgagaacu gaaaaagaac gccgcagagc gggcggagau gaccacaaau     2340

auaaacgcug ccaaguuagu acaugaaaag aucuucaaga uucuccaaaa ugaguuuggg     2400

aucaaaaacc cgacucguaa ugacauuauc agauacaggc uguaugagga acuuaaggga     2460

aacgguuaca aagaucugua uucuaauacc uacauacccc gcgaaauuuu guucagcaac     2520

cagaucgaca uugaucacau ccucccucag ucccggcugu uugacgauuc auucagcaau     2580

aaggucgugg uuuuuagaaa ggacaacuug aaaaagggca aucagacagc aauagauuau     2640

aucgggucug aggugggaga ggaaggccug aaaaacuuca uugagagggu cgaaucccuc     2700

uacgagcugg guaagaaaaa uaaggaggaa gggaucagua aggccaaaua ccaaaaacuu     2760

uugaaaagcg cuucugagau uggcgacgga uuuaucgaac gagaucugcg cgagucccag     2820

uauauagcca agaaagcaaa ggaauuacuc uucaagguga cucggucagu agugaguacc     2880

agcggcucga uuacagacag acugagggag gacugggggc uuaucaacgu uaugaaagag     2940

cugaaucuac caaaguacaa aucccucgga cugaccgaaa uccaggagcg ucgcaacggu     3000

caucaggucg aagugauuaa ggauuggacu aaacggaacg accacagaca ucacgcuaug     3060

gaugccuugg cgguggccuu uacgaagcac aaucauaucc aauaucugaa cuacuuaaau     3120

gcuaggaaaa acgagucuca caagcuccau ggcaauauua uagcaaucga aacacuggag     3180

accgagguca agauugacga ucuugggaac aaaaagcgag uuuucaaaga acccaucccu     3240

aauuuucgcc agguggccaa ggagcaccug gaaaacgugu ugauuagcca caaagcuaag     3300

aauaagguag ucacuaaaaa caagaauaaa aucucaggaa aggagaaacu ccagauaacc     3360

cugacaccac ggggccagcu acauaaggaa accguguaug guaaguacag auucuaucaa     3420

aguaaagacg agaagguuuc cgccaaauuc gaucuggaga cuaucgaaaa gguggcaaac     3480

cccucuuaca aagcggcccu ucuccagagg cugaaugaga acgaaaauga cccgaagaag     3540

gcuuuuagcg ggaaaaacgc cuuguccaag aauccuauuu acaucucaau ggagaacgau     3600

auuaaagucc ccgacacagu gaagcugguu ugguuagagg aagauuauag cauccggaaa     3660

gacgugacgc cagagaacuu caaggacgaa aagaguauag agaagauucu cgaugaaggc     3720

guaaagcgaa uccugaaaaa uagacuuaag gaguuuggag gcgacgcaaa agaggcuuuc     3780

ucugaucugg acaagaaccc uaucugguug aaugaagaga aagggaccuc cauuaagcgc     3840

gucaggauaa gcggagugaa gaacgccgaa ccccuccacu acaaaaagga ucauuuuggc     3900

aaugacaucc uggaugguaa cgggaauaaa auuccagcag acuucguguc gacuggaaac     3960

aaucaccaug ucgccaucua uaaggagccc gaauuugaua aaaagggcca gccuguucua     4020

gacgagcugg guaaccaaaa guacaaucuu caggagaaag ugguaucauu cuuugaagcu     4080

guggagcgug ucaaccaggg ccucccaauu aucgauaaga cauauaauca gcaccugggg     4140

uggcaauucu uuuucaccau gaaacagaac gaaauguucg uguuuccgaa ugagaagaca     4200

cgguucaacc ccauagaaau cgacuuguua gauccuaaca auaaaaagcu gauuucucuc     4260

aaccuguuua gaguucagaa gaucgcgaau aaaaacuaca uguucaggca ccaucuugag     4320

acuaaugugg acgacaagaa agagaugaag aacauugccu uuauccacau acagaguugu     4380

aaucuguuga aagaaauuau caagguccgc cuaaaccauc ugggagauau cguguccguu     4440

ggcgaguauu ga                                                         4452


<210>  3400
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3400
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc caaagugucc       60

gucgaugacc acgguaauua ccugcaugau auaaaguugg gcucuagaau cauucccaug      120

ucacaggaca ucuuagggaa cuucgauaaa ggaguuacug agagucaaac ggcuauuagg      180

accggcuuuc ggagcgugcg ccgaauccgu gaaagauccc ugcuaaggcg ggagcgccuc      240

cacagaguac ugcaugugau ggguuuccuu ccuccacacu augacucugc aauagggugg      300

aauagggaag aggccucgac auacggaaag uuucugaacc auggcgaacc gaaauuggcg      360

ugggagcggc ucgaggaugg cucaaugcgc uuccuguuua uggacagcuu ccacgaaaug      420

auggcuaauu uugcccgaca ccagcccgca cuggucgccg augggagaaa gaucccucuc      480

gacuggacuu uauauuaccu gaggaagaaa gcucuuaguc aggcaguggg aaaggaggaa      540

uuggccugga uucugcuaaa cuucaaucag aaacgggguu auugccaacu ccguggcgag      600

gaggaagagg aaaccccaac aaagcgcgag gaauacuaug agcugaaagu uguguccguc      660

gaggcugccg aagaggggaa gggaggcaac accugguaca gagugcagcu ugaaaauggu      720

uggaucuaca ggcggcccuc uaagauugcg cuggaugacu ggguagggaa aacuaagcag      780

uuuaucguua caaccgagua ugaaaaagau ggaacgacuu ugaagaaaga caaggacggc      840

gaggugaaac gauccuuccg cgcaccuggc gagaacgauu gggggcucag aaagaagagg      900

accgaauccc agcuggaggc cucaggaaaa acagucggug cuuuuauaua cgaccauuua      960

cuggccgaac cagcagauaa gauucggggc aauuucaucc gcacuauuga gagaaaauau     1020

uacaagcaag aacucgaggc uauccuuagg gagcagacca aacaccauga agaacugcgu     1080

aguaaggaaa uguuggccaa gugugcggag gagcuauauc agagcaacca gccccaccgg     1140

aaaucucugc ucaauaagga caugacauac cugcuucuga ccgauuugau cuuuuaucaa     1200

cgaccucuca aauccaagaa aucacugauu gccgaaugcc cauacgagua cuauaaguac     1260

aaggacaaag aaaaggggaa aaagaaaaga cagggaauaa aguguguggc aaagagcaac     1320

ccguauuacc aggaguucag guuauggcaa uucaucguga aucugcgccu auuugaucgg     1380

acggacgaua aagaagucac ugcugaguau cucagaagua gggaggacua cgcccgccug     1440

uucgcuuauc uuaacgaucg aaaggaaauu aaccaggaga cauugcugaa agacuuuuuc     1500

ggccucaaga aaguuaagau cggugccgaa aaagaguuug caauacggug gaauuacauu     1560

gaagacaagg agaagaaaua ccccugcaac gagacccguc acgaacugcu ucuggccuug     1620

gauagagcug ggauggacag gucuuggcuc gaugacuccg agcugcagua ucgcuuaugg     1680

caucugcuau acagcgugga agcgcgggau gaggcagaag gcgcccucag aaagcugagg     1740

gacgaugacg aguucguagg agcuuuucuu aaagugaagc cuuucaaaaa ggauuauggc     1800

gccuacucgg agaaggcagu caaaaaguug cuggccguga ugcgaauggg gucuuggugg     1860

cacgaagagg acaucuguaa acagacucgc gaucggaucg aaagaauugu ucaaggagac     1920

aucgaggaga agauuaggga acguaugggu caugcugccu auuccuuuuc acgccucgca     1980

gauuuccagg gccugccaga gugguuagcg ugcuacgugg ucuaugggcg gcacagugaa     2040

gcuacacaua uacugagaug ggacagcccc gacaaacuuu uguccuacau caagggauuu     2100

aggcagcacu cucuccgaaa uccuauugug gagcagugua uccuggaaac ccuacggacg     2160

guacacgaua ucuggaagga ggccggccau auugacgaga uacacguuga acugggucgc     2220

agcaugaaau caaccgcaga gcaaagagcc cauaugacaa agaacauccu caggaaugaa     2280

aacacuaauc ugcggauuaa augccuuuug auggagcuga agaacgaugc ugacgugaaa     2340

gauguccgac cauacagucc caugcagcag gaaaucuuaa gaauuuauga ggagggggcc     2400

cuccagcaac ugaccaagga cgaugcagaa uacgcugaga ucuccaagau aagcaaaaug     2460

gcccagccgu cugcgaagga acuucugcgc uauaaauugu ggcucgagca gaaguacagg     2520

ucaccuuaua caggcaaacc caucagucug agcaagcuau ucacuuccgc cuaccagauu     2580

gaacacguga ucccacaauc ucguuauuuu gacgauucgu ucaauaacaa agucauuugu     2640

gagagcgagg ugaauacccu gaaguccaac augcucggau acgaguucau caagaaucac     2700

ugcggcgaga uaguucauug uacaaugcug ggggacguga aaguauuuuc agaaagugag     2760

uacaaggcuu ucgucaccga gcacuauucu aacaauaaau acaagaaaga aaaacuuuug     2820

cuggaggaua uuccucagga auuuuuaaac cggcagauga acgacagcag auauaucucc     2880

aaggugguua agggacuccu gucaaaugug gucaggacug aggaugaacu ugaggcaacg     2940

agcaaguucg ugguaucuug cacagguggc auuaccgacc gccugaaaaa ggacuggggg     3000

cuaaacgaug uguggaauag caucguuuac agucgauuug agagacucaa cggacugacu     3060

aaaagcgaac auuucggcca cugggagaau aaggaaggua agcgggucuu ucaaaccucu     3120

gugcccaugg aguugcagag gggguucaac aaaaagcgca uagaccaucg ucaccacgcc     3180

auggaugcuc uggugaucgc cugcgcaucc agaaauauua ucaacuauuu aaauaacgaa     3240

uuugcgaaau caccaaagca gcgggaggac cucaggcagc ugcuuuguga uaaaaaucgc     3300

auuauccgaa agcccuggga gacauucacc caagacgcca gagcugcccu ggaagauguc     3360

guugugaguu uuaagaacua cgugcgggua auaaauaaaa cuacaaacua uuacgagagg     3420

uacgacgcag auggcaagaa aacgaugguc ccucagaagg gaaaugaaau gugggcugug     3480

cgcaaaccaa ugcaugaggg caccauguau gggagaguua acuugaggcg gaaggccgug     3540

gucaagcuca aagaagcacu ggagaacauu agcucgaucu gcaauaagga gcuacgugac     3600

uacaucaacg ugcugguuga aaaacaguuc aauacuaagc agcuucucgc ccacuuuaaa     3660

uccauggauu aucgcuggaa caagcaacau auugacaaag uggagguaug gcgauucucu     3720

gacgaaaagg uccagauggu ggcugcgaga aagccgcugg auaccagcuu cgacaaagag     3780

uguaucgccu caauuacaga uacaggaaua cagaagaucu ugcugaauua cuuagagagu     3840

aaaggcggug accccgcaau ugcuuuuacu uccgaaggga ucgccgagau gaaccagaau     3900

auugccgugu auaacgaugg aaagaaacac caaccuguca ucaagguuag gaagucuacc     3960

aaacucggca agaaauucgc ugugggucag auaggcugca agagcaaaca guuuguagug     4020

gcagaagccg ggacgaaucu guacuucgcg aucuaugagg acgaagaggg aaagcggucc     4080

uacgauacca uuccacuuaa cgaggucaca gaacgccuga agcaaggcuu gucacccgug     4140

ccugagaaaa augaaaagga gguuccacuc aaauuuuacc ugagccccaa cgaccuagug     4200

uaugucccga cugaagauga gaugcagaug aaggagugua aacugaauaa ggaaagaauc     4260

uacaagaugg ugaguucuac cgggaacgag ugccauuucc uuccuuauuc cguugcuagc     4320

gugauuuaca auaaagucga auuugagucg augaacaaga ugggaagggc ccucacaggc     4380

gaaaugauca aagcaguaug uuggaagcug gacguggauc gguuggguaa cauauuacga     4440

guuauuaaau ga                                                         4452


<210>  3401
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3401
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc caaaauaucc       60

aucgaugaca augguaacua ccugagagau auucaguugg gcucuaggau cauucccaug      120

ucacaagacg uguuagggaa uuucgauaac ggagucacug agagucagac ggcuguucgg      180

accggcuuuc gcggucugcg acguaucaga gaaaggagcc uacuccggcg cgagagacug      240

cacagggugc uucauguaau gggguuccug ccuccacacu augacuccgc aauaggaugg      300

aauaaggaag aaccgacaac uuacggcaaa uuuuugaacc auggcgaacc caagcucgcc      360

ugggagcggc agggggaugg aucuaugcgc uuccuguuua uggacucguu ccacaaaaug      420

auggaggauu uugcgccuca ucagccagcu cugguggccg acggucgaaa gaucccccuc      480

gauuggaccu uauauuaccu gagaaagaaa gcacuuacac aaccuauuaa gaaagaagag      540

uuggccugga uccugcuaaa uuucaaccag aagaggggcu auuaccagcu ccguggggaa      600

gaggaggaag agaauccaac caaacgggaa gaguaucacg aacugaaggu cgugggaguu      660

gaggcugcag aggaaggcaa gggugggaac acuugguaca augugaaacu ugagaacgga      720

uggauuuacc gcagaucaag caagaucccc cuggacgauu gggucggcaa aacaaggccu      780

uuuauaguga ccacggaaua ugaggccgac ggcauuacuu ugaagaaaaa uaaggaaggg      840

gagguaaaac ggaguuuccg agcuccagga gaggacgauu ggggucucug gaagaagcgc      900

accgaauccc agcuggaggc cucuggcaaa acaguugggg cguuuaucua cgaccacuua      960

cuggcagaac cggccgauaa gauuagagga aacuucauca ggacuaucga gcggaaauau     1020

uacaagcaag aacucgcuga cauucuucgc gagcagagca aauaucauga ggaacugcag     1080

uccaagaaga uauuggccga uugcgcagag gaacuauaca gaucaaauca gccccacagg     1140

gacagucugc ucaaaaagga uaugguguau cugcuucuga ccgacuugau cuuuuaccaa     1200

cguccucuca aaagcaagaa aucucugauu gcugauuguc cauacgagua uuacgaguau     1260

gucgacaagg aaacaggcga gaucaagcgg caggguauaa aaugcauugc caaguccaac     1320

cccuacuauc aggaguuccg auuauggcaa uucgugcuga accuaagacu cuacgauagg     1380

accgaggaca aagaagugac ggcggaguau cugccuucaa aggaggacua cgcccgccuu     1440

uuugucuacu ugaaugaucg gaaagaaauc aaccaggaga cucugcucaa ggacuucuuu     1500

aagcugaaaa agguuaaaca gagaagcgaa aaggauuucc cagugaggug gaauuauauc     1560

gaggacaaag aaaagaaaua ccccuguaac gagacacgcc augagcuucu gauggcauug     1620

gaucgagcug ggauugaacg gaguuggcuc gacgauaccg cccgucagua uagacugugg     1680

cacuuacugu acucuaucga gguaaaggac gaagcugaga gggcccuacg caagcucaaa     1740

gaugacgaug aauuuguguc cgcauuccug aaggucaaac cguuuaagaa agacuauggc     1800

gccuacagcg agaaggcugu gaagaaacuu uuggcgguua ugcggauggg aucgcacugg     1860

ugcgaggaug acauuuguaa ggaaacuaaa gagagaauag aacauauccu gcaaggcgac     1920

gugggggaga agauuaaaga gaggaugaau cacacagcau auucucucgg agaacugucc     1980

gauuuccagg ccuuaccuga guggcuggcu ugcuacgucg uguacggucg acauucagaa     2040

gccaccgaca ucaagcacug gaagacgccc gagagucuua uggcauauau caacggcuuu     2100

aaacagcaua gcuugcgcaa uccaauugua gaacagugua uacucgagac ccugcggaca     2160

guucacgaua ucuggaagga ggccgggcac auugacgaaa uccaugugga gcuaggaaga     2220

uccaugaaau cuacuagcga ucaaagggcu cguaugaccg aaaacauucu gcgcaaugag     2280

aacacaaauc uccggaucaa gucacugcuu auggaauuga aaaacgacgc cgauauaaag     2340

gacgucagac cuuacagucc caugcagcag gagauccuga ggauuuauga ggaaggcgca     2400

uuacaggagc ucacuaagga agauaaugac uacgaggaaa ucuucaaaau uuccaagaug     2460

ccacaaccca gcgcgaaaga gcugcuuaag uauaaacugu gguuggagca gaaguaccga     2520

ucuccuuaua ccgguaaacc aaucucacuc aguaagcugu uuacaagcgc uuaccagaua     2580

gaacacauua ucccgcaguc ccgguacuuc gaugacucuu uuaacaauaa ggugauuugc     2640

gagucggaag ucaacgcccu aaaagggaac augcugggcu augaguucau caagaaucau     2700

ggaggcgaga uagugcacug uacccuccug ggggauguua aagugcaaaa cgaaagcgag     2760

uacaaggcau ucguaacuga acacuaugcc aauaacaaau acaagaagga gaaacuuuug     2820

auggaagaca ucccccagga cuuucugaau cgccauuuaa acgauuccag auauauuuca     2880

aaggucguga aaacgcuccu gaguaauauc guuaggacag aggacgagau ggaaaccacu     2940

ucuaaguucg uggucagcug caccggaggu auuacagauc ggcuuaaaaa ggacuggggc     3000

cugaacgaug uguggaacuc caucguauac ucacgauuug agagacuaaa ugaacucacc     3060

aagagcgacg cuuucgggcg cugggagaau aaagaaggaa agaggguguu ucagacuucu     3120

guuccucugg aguugcaacg uggcuucaac aaaaagcgga uagaucacag acaucacgcc     3180

auggacgcac uggucauugc uugugccucc aggaauauca ucaacuauuu aaauaacgag     3240

agugcgaaca gccccaaaaa gcgcgaagau cuccgacagc agcugugcga caagaauaga     3300

auuauccgga aaccauggga gucuuuuaca caggaugcca gggcugcacu ugaagacgug     3360

guggucuccu ucaagaacua cguucgcgug auuaauaaag ccacgaacua cuaugagcgu     3420

uacgacgcug augguaagaa aaccauaacu aagcaaaagu caagagaaau gugggccgug     3480

cggaaaccua ugcauaagga gacaguauuu gggcacguca aucugaggcg caaagcaauc     3540

gugaaguuga aagacgcgcu cgagaacauu ccaaguaucu gcaauaagga acugcgagau     3600

uauauuaacg gccuaguuga gagaaauuuc aacaccaaac agcugcacgc ccauuuucgg     3660

agcacagacu acagguggaa uaagcagaac guggauaagg ucgaagugug gcgcuucucg     3720

gacgagaaag agccccuugu ugcuacuaga aagccgcucg auaccuccuu ugacgccaaa     3780

aggaucgaua cgauaaccga cacaggaauc cagaagauuc uguugaauua ucuggaagca     3840

aaaggcgggg auccuuuaau cgcuuucacu ccagagggaa uugccgaaau gaaccaaaau     3900

aucgcacucu acaacgacgg caagaugcac cagcccauac ugaaggugcg gauuucugag     3960

ccuaaaggug ccaaguauca gaucgggcag accggaaaca aagcugauaa guucguagaa     4020

gcgcaaaaag gcacaaaucu uuacuuugcc aucuacgagg acgaggaccg uaaccgcacu     4080

uauaauacca uuccccugaa cgaagucgca gagcgauuga agcagggucu cccaccugug     4140

cccgaaaaga augagaaaga agugccacug aaguucuacc uaagcccgaa cgaucugguc     4200

uauguuccua cggaggagga aauacuuaug aaagagugua agcucaauaa aaacagaauc     4260

uacaagaugg ugucaaguac aggcaaucag ugcuuuuuca uucccuuuag gguagcugug     4320

uccaucguca acaagaugga auauucucca cugaauaaaa uggagaaggc cauuagcucc     4380

gaaaugauca aagccgugug uuggaaguug gagguugacc gguuagggaa cauaauccgc     4440

guggucaaau ga                                                         4452


<210>  3402
<211>  4458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3402
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugggc caaaauaucc       60

aucgaugaca acgguaagua cauuaaagau ucuaucaagc ugggcucaag aauuaucccc      120

augagucagg acguguugag caauuucucc aaagggguca cauuaucuca aacuucggag      180

aggacgucau uucggggagc ucgccgacug cgugaaagaa cccuacucag gcgggagcgc      240

cugcacagaa uacuucaucu guugggcuuc cucccuaagc acuaugaaaa ggcaaucggu      300

ugggauagga acgacaauaa aacauacggg aaguuuauua accaugccga gccaaaacug      360

gcguggugca aggauaauaa cggaaaaaug cgguuccugu uuauggacag cuuccacgau      420

augcucagug aauuuuccga gaagcauccg gaguuacugg cuaauggcaa gaaaaucccc      480

cuugacugga cuauuuauua cuugcgcaag aaagcccugu auaaggcaau caccaaagag      540

gaacuagccu ggauacuccu gaacuucaau cagaagcgag gcuacuauca gcuuagaggg      600

gaagaggaua gggacucuga ggcuaacaaa aaggaauacc uggaguugaa gguuauugga      660

guggaagcaa caggugagaa aaagggcaau gauaucuggu acaacauuca ccucgaaaau      720

ggguggaucu aucggcguag cuccaaaaac ccucugaacg acugguuagg aaagguaaaa      780

ucauuuaucg ugaccacuga ccuggaugcc gacggcaauc caaagaagga uaaagacggu      840

aacauuaagc gcaguuucag agcucccagc gagggggauu ggacacucau caaaaagagg      900

accgagucuc uucuggaaaa uuccggacag acggucggcc aauuuauaua caaccacauu      960

uuggaggaac cuucagccaa aauccggggc aauuucauac gaacuauuga gcgcaaguau     1020

uacaaggacg aacuaaaagc gauccuggag aagcagaaag aguuucauca cgaacucagc     1080

agugagucuc ugcuuaccga augugcaaag gagcuguaua aaagaaacau cgcccaucag     1140

gaaguguugc ucaagaaggg gaugauucac cuguuacuga augauauccu auucuaccag     1200

aggccacuca aaauuaagaa aucccugaua gacaacugcc ccuaugagaa uuacaaguac     1260

guugauaaaa agagcggaga caucaaagug aagucgauua aguguaucgc uaaaucuaac     1320

ccuuucuuuc aagaguuccg gcuuuggcag uuugucucca auuugcgccu guauagaaag     1380

ucagaugaca aagacgugac aagugaacac cucguaacua ccgaugacua cgccaaccug     1440

uucacauauc uuaauaacag gaaggauauc aaacagagcg uucuguugaa ggaguacuuu     1500

aagauuaaaa aggugaaaac cauaaauggu gcagaauauc caguccguug gaacuacauc     1560

gacgaugaca acaagacgua uccgugcaau gagacucggu acgauauucu ccaugcucug     1620

uccaaaacag aguuaaaguc ugaauggcug acccccgagc gaacuuacag acuauggcac     1680

cuccuguaua gcaucgaauc aaaggaggaa accaaaaagg cccuuaacaa auugaaucug     1740

agugacgauu ucacagaggc guuucucacg auuaagccuu ucaaaaagga guacggcucc     1800

uauagcgaaa aggccaucaa aaagcuguua ucucugauga ggauggggua ccuuugguca     1860

gaggacgaag caauaaaaca ggugggagcu gagaucgaaa agaccauuga gggcgaguug     1920

ccagauuaug ugcuccaacg ccuggaaaaa ucacagcaua acuacaauaa gaucagcgac     1980

uuuuccgguc uaccccugug gcucgccucu uauauuaucu acgggcggca cucggaggcu     2040

aaagaaauaa uucauuggga uaacccugag agcaugaugg ccuacaucaa ggacuucaga     2100

cagcacucca ugaggaaucc aauugucgaa caauguguuc uggagacacu ucgcacugug     2160

cacgacaucu ggaaggagau aggccauauc gaugaaauuc acaucgaguu gggacgagaa     2220

augaaaucaa ccagugagca gcggaaggca auuacacaga acguacugca uaaucagaac     2280

acuaauaucc guauaaaauu acuccugcaa gagcuuaaga acgacaauaa cauuaccggc     2340

gugagacccu auucuccgau gcagcaggaa auccugagga ucuacgagga aggggccuug     2400

cagggacuca caaaagagga uaaggacuau aaugauauuc ugaagaucag ccaaaccgcu     2460

cagccuacuu ccaacgaacu aacgcgcuac aaacuguggc ucgagcagaa guaucgguca     2520

cccuacacag guaaagcgau uagccugucu aaacuuuuua ccuccgcaua ucagauagag     2580

cacgucaucc cacaaaguag auacuucgac gauagcuugu cuaacaaagu gauuugcgaa     2640

uccgagguua auacucugaa gaaggccaug uuaggcaugg aauuuaucaa aaccuguggg     2700

ggacagaagg ugcacugcac acuccugggc gacgucgaga uuuacaacga agaggauuau     2760

aaggcuuucg ugaaugagca uuacucaaac aauaggacca agcgaaacaa acuucugaug     2820

gaagacaucc cucaggaguu cauaaaucgc cagcuaaacg auagucggua uaucagcaag     2880

augguaaagc aacuccuguc ggccauuguu agaggucagg aagaggugga agcaacuucc     2940

aaauuuguca uccccuguac agggggcauu acggacaggu ugaagaaaga uuggggacug     3000

aaugacaucu ggaacaauuu agugucucca cguuuccagc gcaugaacgc ccucaccggc     3060

agcgaguacu uugggcacug ggagaauaag gaaggaaaac gggucuucca aacuacaaug     3120

cccauacagc ugcagcaggg uuuuucaaag aagagaauug accauaggca ccaugcuaug     3180

gaugcccuug ugaucgcaug cgcgagucga aacaucauuu ccuaucugaa uaacgagacc     3240

gcucaagaca cagaacggcg cagagauuug aggcagaaac ucugugacaa gggcgggucu     3300

guucggaaac cuugggauag cuucacucag gacacccagu acgcccugaa ggauauaauc     3360

gugacguuua aaaacuaugu acgaauuauc aauaaggcaa ccaacuacua cgagcacuau     3420

aaaacagacg gaaagaaggg caugauuucc caaaaaggug augagcuaug ggccaucaga     3480

aagccaaugc acaaagaaac ugucuucggg aaggugaauc ugcgcaggcc gaaacguguu     3540

ucacuuagca aggcuauaga caguguggau gucaucugca accggaagcu caaaaaguac     3600

auuaaagcca gacugaauga gggcuugcag aagaaacaga uccugaacau uuuuaaggaa     3660

gugaauaacg uaugggaggg aaucgacgug aauaagauag acauuuggga aaugucuaac     3720

gauaaagagc ccaugguugc aaccaggaag caguuagaca cauccuucga ugagaaaaag     3780

auccgcagca ucucggacac uggcauucag aaaauccucc ugaauuaucu uauaaccaag     3840

aacaauaacc cugaacuggc uuuuacgcca gaggggauuc aagaaaugaa uaguaacauc     3900

ucugaguaca augaucauaa gaaacaccag cccauuuaua agguccgagu gacagaaccu     3960

uugggagcca aauacccaau cggcaccaug gguaacaacc ccaagaaauu cguggaggcg     4020

caagccggga cuaaucucua uuuugcuaua uacgaggaca aggaaggaaa cagaaauuac     4080

gauacaauuc cgcugaacau cgcagccgag cggcuaaauc agcagcugag uccuguccca     4140

gaaaccaacg agaauaacac uccccuuaaa uucuaucucu ccccuaauga ccugguuuac     4200

gugcccagcg aagaggagag gcauucuggc aacaauaucc aguuggauaa gaaccgcauu     4260

uauaagaugg ugagcuccac cucaaaacaa ugcuucuuua uccaccaucg uguagcuauu     4320

aguuuagucg acaagaaaga guucagcgcc cagaauaaga uggaaagauc uauagauaac     4380

gaacagguga ugaucaaauc cguuuguugg aagcugauua cagaccggcu cgguaauauc     4440

aucaagguga uuaaauga                                                   4458


<210>  3403
<211>  4458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3403
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugggc caaaauaucc       60

aucgaugaca acgguaagua cauuaaagau ucuaucaagc ugggcucaag aauuaucccc      120

augagucagg acguguugag caauuucucc aaagggguca cauuaucuca aacuucggag      180

aggacgucau uucggggagc ucgccgacug cgugaaagaa cccuacucag gcgggagcgc      240

cugcacagaa uacuucaucu guugggcuuc cucccuaagc acuaugaaaa ggcaaucggu      300

ugggauagga acgacaauaa aacauacggg aaguuuauua accaugccga gccaaaacug      360

gcguggugca aggauaauaa cggaaaaaug cgguuccugu uuauggacag cuuccacgau      420

augcucagug aauuuuccga gaagcauccg gaguuacugg cuaauggcaa gaaaaucccc      480

cuugacugga cuauuuauua cuugcgcaag aaagcccugu auaaggcaau caccaaagag      540

gaacuagccu ggauacuccu gaacuucaau cagaagcgag gcuacuauca gcuuagaggg      600

gaagaggaua gggacucuga ggcuaacaaa aaggaauacc uggaguugaa gguuauugga      660

guggaagcaa caggugagaa aaagggcaau gauaucuggu acaacauuca ccucgaaaau      720

ggguggaucu aucggcguag cuccaaaaac ccucugaacg acugguuagg aaagguaaaa      780

ucauuuaucg ugaccacuga ccuggaugcc gacggcaauc caaagaagga uaaagacggu      840

aacauuaagc gcaguuucag agcucccagc gagggggauu ggacacucau caaaaagagg      900

accgagucuc uucuggaaaa uuccggacag acggucggcc aauuuauaua caaccacauu      960

uuggaggaac cuucagccaa aauccggggc aauuucauac gaacuauuga gcgcaaguau     1020

uacaaggacg aacuaaaagc gauccuggag aagcagaaag aguuucauca cgaacucagc     1080

agugagucuc ugcuuaccga augugcaaag gagcuguaua aaagaaacau cgcccaucag     1140

gaaguguugc ucaagaaggg gauuauccac cuguuacuga augauauucu auucuaccag     1200

aggccacuca aaauaaagaa aucccugauc gacaacugcc ccuaugagaa uuacaaguac     1260

guugauaaaa agagcggaga cauuaaagug aagucgauca aguguaucgc uaaaucuaac     1320

ccuuucuuuc aagaguuccg gcuuuggcag uuugucucca auuugcgccu guauagaaag     1380

ucagaugaca aagacgugac aagugaacac cucguaacua ccgaugacua cgccaaccug     1440

uucacauauc uuaauaacag gaaggauauu aaacagagcg uucuguugaa ggaguacuuu     1500

aagaucaaaa aggugaaaac cauaaauggu gcagaauauc caguccguug gaacuacauu     1560

gacgaugaca acaagacgua uccgugcaau gagacucggu acgauauccu ccaugcucug     1620

uccaaaacag aguuaaaguc ugaauggcug acccccgagc gaacuuacag acuauggcac     1680

cuccuguaua gcauugaauc aaaggaggaa accaaaaagg cccuuaacaa auugaaucug     1740

agugacgauu ucacagaggc guuucucacg aucaagccuu ucaaaaagga guacggcucc     1800

uauagcgaaa aggccaucaa aaagcuguua ucucugauga ggauggggua ccuuugguca     1860

gaggacgaag caauaaaaca ggugggagcu gagauugaaa agaccaucga gggcgaguug     1920

ccagauuaug ugcuccaacg ccuggagaaa agucagcaua acuacaauaa gauuagcgac     1980

uuuuccgguc uaccccugug gcucgccucu uauaucauau acgggcggca cucggaagcu     2040

aaagaaauua uccauuggga uaacccugag agcaugaugg ccuacauuaa ggacuucaga     2100

cagcacucca ugaggaaucc aaucgucgaa caauguguuc uggagacacu ucgcacugug     2160

cacgacauau ggaaggagau cggccauauu gaugaaaucc acauugaguu gggacgagaa     2220

augaaaucaa ccagugagca gcggaaggca aucacacaga acguacugca uaaucagaac     2280

acuaauauac guauuaaauu acuccugcaa gagcuuaaga acgacaauaa caucaccggc     2340

gugagacccu auucuccgau gcagcaggaa auccugagga uuuacgagga aggggccuug     2400

cagggacuca caaaagagga uaaggacuau aaugauaucc ugaagauuag ccaaaccgcu     2460

cagccuacuu ccaacgaacu aacgcgcuac aaacuguggc ucgagcagaa guaucgguca     2520

cccuacacag guaaagcgau aagccugucu aaacuuuuua ccuccgcaua ucagaucgag     2580

cacgucauuc cacaaaguag auacuucgac gauagcuugu cuaacaaagu gaucugcgaa     2640

uccgagguua auacucugaa gaaggccaug uuaggcaugg aauuuauuaa aaccuguggg     2700

ggacagaagg ugcacugcac acuccugggc gacgucgaga ucuacaacga agaggauuau     2760

aaggcuuucg ugaaugagca uuacucaaac aauaggacca agcgaaacaa acuucugaug     2820

gaagacauac cucaggaguu caucaaucgc cagcuaaacg auagucggua uauuagcaag     2880

augguaaagc aacuccuguc ggccaucguu agaggucagg aagaggugga agcaacuucc     2940

aaauuuguca uucccuguac agggggcauc acggacaggu ugaagaaaga uuggggacug     3000

aaugacauau ggaacaauuu agugucucca cguuuccagc gcaugaacgc ccucaccggc     3060

agcgaguacu uugggcacug ggagaauaag gaaggaaaac gggucuucca aacuacaaug     3120

cccauucagc ugcagcaggg uuuuucaaag aagagaaucg accauaggca ccaugcuaug     3180

gaugcccuug ugaucgcaug cgcgagucga aacauuauau ccuaucugaa uaacgagacc     3240

gcucaagaca cagaacggcg cagagauuug aggcagaaac ucugugacaa gggcgggucu     3300

guucggaaac cuugggauag cuucacucag gacacccagu acgcccugaa ggauaucauu     3360

gugacguuua aaaacuaugu acgaaucauu aauaaggcaa ccaacuacua cgagcacuau     3420

aaaacagacg gaaagaaggg caugaucucc caaaaaggug augagcuaug ggccauaaga     3480

aagccaaugc acaaagaaac ugucuucggg aaggugaauc ugcgcaggcc gaaacguguu     3540

ucacuuagca aggcuaucga caguguggau auuaucugca accggaagcu caaaaaguac     3600

auuaaagcca gacugaauga gggcuugcag aagaaacaga uccugaacau auuuaaggaa     3660

uuaaauaacg ucugggaggg aauugacgug aauaagaucg acaucuggga aaugucuaac     3720

gauaaagagc ccaugguagc aaccaggaag cagcucgaca cauccuucga ugagaaaaag     3780

auucgcagca ucucggacac uggcauacag aaaauucugc uuaauuaucu gaucaccaag     3840

aacaauaacc cugaauuggc uuuuacgcca gaggggauuc aagaaaugaa cucaaauauc     3900

ucugaguaca augaucauaa gaaacaccag cccauauaua aggugcgagu uacagaaccu     3960

cucggagcca aauacccaau uggcaccaug gguaacaacc ccaagaaauu cgucgaggcg     4020

caagccggga cuaaucugua uuuugcuauc uacgaggaca aggaaggaaa aagaaacuac     4080

gauacaaucc cgcuaaauau ugcagccgag cggcugaacc agcagcuuag uccugugcca     4140

gaaaccaaug agaacaauac uccccucaag uucuaucugu ccccuaacga cuugguguac     4200

guccccagcg aagaggagag gcauucuggc aauaacaucc aguuagauaa gaaucgcauu     4260

uauaaaaugg uuagcuccac cucaaagcgu ugcuucuuua uacaccauag aguggcuauc     4320

agucuggugg acaaaaagga guucagcgcc caaaacaaaa uggaacgguc uauugauaau     4380

gaacagguaa ugaucaaguc cgucuguugg aagcucauca cagacaggcu ggguaacauu     4440

auaaaaguga ucaaguga                                                   4458


<210>  3404
<211>  4458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3404
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugggc caaaauaucc       60

aucgaugaca acgguaagua cauuaaagau ucuaucaagc ugggcucaag aauuaucccc      120

augagucagg acguguugag caauuucucc aaagggguca cauuaucuca aacuucggag      180

aggacgucau uucggggagc ucgccgacug cgugaaagaa cccuacucag gcgggagcgc      240

cugcacagaa uacuucaucu guugggcuuc cucccuaagc acuaugaaaa ggcaaucggu      300

ugggauagga acgacaauaa aacauacggg aaguuuauua accaugccga gccaaaacug      360

gcguggugca aggauaauaa cggaaaaaug cgguuccugu uuauggacag cuuccacgau      420

augcucagug aauuuuccga gaagcauccg gaguuacugg cuaauggcaa gaaaaucccc      480

cuugacugga cuauuuauua cuugcgcaag aaagcccugu auaaggcaau caccaaagag      540

gaacuagccu ggauacuccu gaacuucaau cagaagcgag gcuacuauca gcuuagaggg      600

gaagaggaua gggacucuga ggcuaacaaa aaggaauacc uggaguugaa gguuauugga      660

guggaagcaa caggugagaa aaagggcaau gauaucuggu acaacauuca ccucgaaaau      720

ggguggaucu aucggcguag cuccaaaaac ccucugaacg acugguuagg aaagguaaaa      780

ucauuuaucg ugaccacuga ccuggaugcc gacggcaauc caaagaagga uaaagacggu      840

aacauuaagc gcaguuucag agcucccagc gagggggauu ggacacucau caaaaagagg      900

accgagucuc uucuggaaaa uuccggacag acggucggcc aauuuauaua caaccacauu      960

uuggaggaac cuucagccaa aauccggggc aauuucauac gaacuauuga gcgcaaguau     1020

uacaaggacg aacuaaaagc gauccuggag aagcagaaag aguuucauca cgaacucagc     1080

agugagucuc ugcuuaccga augugcaaag gagcuguaua aaagaaacau cgcccaucag     1140

gaaguguugc ucaagaaggg gaugauucac cuguuacuga augauauccu auucuaccag     1200

aggccacuca aaauuaagaa aucccugaua gacaacugcc ccuaugagaa uuacaaguac     1260

guugauaaaa agagcggaga caucaaagug aagucgauua aguguaucgc uaaaucuaac     1320

ccuuucuuuc aagaguuccg gcuuuggcag uuugucucca auuugcgccu guauagaaag     1380

ucagaugaca aagacgugac aagugaacac cucguaacua ccgaugacua cgccaaccug     1440

uucacauauc uuaauaacag gaaggauauc aaacagagcg uucuguugaa ggaguacuuu     1500

aagauuaaaa aggugaaaac cauaaauggu gcagaauauc caguccguug gaacuacauc     1560

gacgaugaca acaagacgua uccgugcaau gagacucggu acgauauucu ccaugcucug     1620

uccaaaacag aguuaaaguc ugaauggcug acccccgagc gaacuuacag acuauggcac     1680

cuccuguaua gcaucgaauc aaaggaggaa accaaaaagg cccuuaacaa auugaaucug     1740

agugacgauu ucacagaggc guuucucacg auuaagccuu ucaaaaagga guacggcucc     1800

uauagcgaaa aggccaucaa aaagcuguua ucucugauga ggauggggua ccuuugguca     1860

gaggacgaag caauaaaaca ggugggagcu gagaucgaaa agaccauuga gggcgaguug     1920

ccagauuaug ugcuccaacg ccuggaaaaa ucacagcaua acuacaauaa gaucagcgac     1980

uuuuccgguc uaccccugug gcucgccucu uauauuaucu acgggcggca cucggaggcu     2040

aaagaaauaa uucauuggga uaacccugag agcaugaugg ccuacaucaa ggacuucaga     2100

cagcacucca ugaggaaucc aauugucgaa caauguguuc uggagacacu ucgcacugug     2160

cacgacaucu ggaaggagau aggccauauc gaugaaauuc acaucgaguu gggacgagaa     2220

augaaaucaa ccagugagca gcggaaggca auuacacaga acguacugca uaaucagaac     2280

acuaauaucc guauaaaauu acuccugcaa gagcuuaaga acgacaauaa cauuaccggc     2340

gugagacccu auucuccgau gcagcaggaa auccugagga ucuacgagga aggggccuug     2400

cagggacuca caaaagagga uaaggacuau aaugauauuc ugaagaucag ccaaaccgcu     2460

cagccuacuu ccaacgaacu aacgcgcuac aaacuguggc ucgagcagaa guaucgguca     2520

cccuacacag guaaagcgau uagccugucu aaacuuuuua ccuccgcaua ucagauagag     2580

cacgucaucc cacaaaguag auacuucgac gauagcuugu cuaacaaagu gauuugcgaa     2640

uccgagguua auacucugaa gaaggccaug uuaggcaugg aauuuaucaa aaccuguggg     2700

ggacagaagg ugcacugcac acuccugggc gacgucgaga uuuacaacga agaggauuau     2760

aaggcuuucg ugaaugagca uuacucaaac aauaggacca agcgaaacaa acuucugaug     2820

gaagacaucc cucaggaguu cauaaaucgc cagcuaaacg auagucggua uaucagcaag     2880

augguaaagc aacuccuguc ggccauuguu agaggucagg aagaggugga agcaacuucc     2940

aaauuuguca uccccuguac agggggcauu acggacaggu ugaagaaaga uuggggacug     3000

aaugacaucu ggaacaauuu agugucucca cguuuccagc gcaugaacgc ccucaccggc     3060

agcgaguacu uugggcacug ggagaauaag gaaggaaaac gggucuucca aacuacaaug     3120

cccauacagc ugcagcaggg uuuuucaaag aagagaauug accauaggca ccaugcuaug     3180

gaugcccuug ugaucgcaug cgcgagucga aacaucauuu ccuaucugaa uaacgagacc     3240

gcucaagaca cagaacggcg cagagauuug aggcagaaac ucugugacaa gggcgggucu     3300

guucggaaac cuugggauag cuucacucag gacacccagu acgcccugaa ggauauaauc     3360

gugacguuua aaaacuaugu acgaauuauc aauaaggcaa ccaacuacua cgagcacuau     3420

aaaacagacg gaaagaaggg caugauuucc caaaaaggug augagcuaug ggccaucaga     3480

aagccaaugc acaaagaaac ugucuucggg aaggugaauc ugcgcaggcc gaaacguguu     3540

ucacuuagca aggcuauaga caguguggau gucaucugca accggaagcu caaaaaguac     3600

auuaaagcca gacugaauga gggcuugcag aagaaacaga uccugaacau uuuuaaggaa     3660

uuaaauaacg ugugggaggg aaucgacgua aauaagauag acauuuggga aaugucuaac     3720

gauaaagagc ccaugguggc aaccaggaag cagcucgaca cauccuucga ugagaaaaag     3780

auccgcagca ucucggacac uggcauucag aaaauccugc uuaauuaucu gauaaccaag     3840

aacaauaacc cugaauuggc uuuuacgcca gaggggauuc aagaaaugaa uaguaacauc     3900

ucugaguaca augaucauaa ggaacaccag cccauuuaua aaguucgagu cacagagccu     3960

cucggagcca aguacccaau cggcaccaug gguaacaaac ccaagaaauu cguggaggcg     4020

caagccggga cuaaccugua uuuugcuaua uacgaagaca aggagggaaa uagaaacuac     4080

gauacaauuc cgcuaaauau cgcagccgaa cggcugaacc agcagcuuag uccugugcca     4140

gagaccaaug aaaacaauac uccccucaaa uucuaucugu ccccuaacga cuuggucuac     4200

guucccagcg aggaggaaag gcauucuggc aauaacaucc aguuagauaa gaaucgcauu     4260

uauaagaugg ugagcuccac cucaaaacaa ugcuucuuua uccaccaucg uguggcuauu     4320

agucugguag acaagaaaga guucagcgcc cagaacaaga uggaaagauc uauagauaau     4380

gagcagguca ugaucaaauc cguguguugg aagcucauua cagaccggcu ggguaacauc     4440

aucaagguua uuaaauga                                                   4458


<210>  3405
<211>  4461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3405
augaccaaga uccugggccu cgaccuuggg acaaacagca uuggaugggc cauaagagag       60

gaugaaaaug agggugugaa ccagaucauu gacaaaggcg ucaggaucuu cuccgaaggg      120

guuaagucug agaauggaaa agaaauuuca cgggcugcag agcgcacugc cuaccgaagu      180

gcgcguaaga ucaaauauag aaggaagcug cggaaguacg agacguugaa aguguuaagc      240

guaaacggca ugugcccccu guccguggaa gaggucgaac aauggaagaa aucugguuuu      300

aaggaguauc cucuaaaucc agaguuccuc aacuggcugc gcaccaauga agauaaaaac      360

auaaauccgu acuuuuucag agacaaggcu ucgaagcaga aagugacacu uuuugagcug      420

gggagggccu uguaucacau cgcacagcgg cgcggauucc ucucaaaccg acuggaucag      480

agcgccgaag gcguuuuuga ggaacauaau ccccaaauuc agaaccugau cgaggaccuc      540

gauaguucca auacuauuuu aaacgagcug aaggaauacu auaucaaucu uggcauaauu      600

gacgagucug aaaagagcgg guucaagaaa gauuuggacg agggagaaaa gaaccugaaa      660

aagcuauaca ccucccucgu gucaaucaca aagaaaaacg cuaaugauau ugagaccugu      720

aagcaggagc ugaucgcaag acuuaacaaa aaggaagacc uggguaaagu caagggcgag      780

aucaaggaua uuagucaggc cauguuggac gggaauuuua aaacucucgg acaauacuuc      840

uuuagccugu auaacaagac aaggauacgg aaucaguaca ccucucguga ggagcacuau      900

uuagaagagu ucaucauuau cugccagacg cagggcauag aggaaauuaa cacuaaugag      960

aaacugccug aaaagaaauu uaccggucuc gcuaaggacc uuuaccgcgc caucuucuuc     1020

caaagaccac ugaaguccca gaaaggguug aucggaaagu guucauuuga gaaaaacaag     1080

aguaggugcg cggugagcca ucccgauuau gaggaguucc ggaugugguc uuaccuaaau     1140

acaauuaaaa ucggcacuca guccgaaaag acccugcgau uucucacacu ggaagagaaa     1200

cuuaaacugg uaccuaaguu cuaucgcaaa aacgacuuua auuucgaagu uuuggcaaag     1260

gagcucgugg agaaaggcgc cagcuuuggg uacuacaagu cgucuaaaaa gaacgaguuc     1320

uuuuauuggu ucaauuacaa gccaaccgau uccgucucag cuuguguggu gagugccagc     1380

cuggaaaacg caauuggaaa agacuggaag acgaaaacuu uugaauauca aacauccaau     1440

accaagaaag aggaagucac uaagucuguu aacuacaagg auuuauggca ucugcuaagc     1500

guggcuacau cagacaccua ucucuacgau uucgccauag agaaacugaa acuugagccc     1560

aaaaacgcga aggccuuuag uaaaacgaag uugaagaaag acuucgcauc ccugagccuc     1620

gcugccaucg cuaagauucu gccuuaucuu aaacaggguc uguuguacuc ucacgccgua     1680

uucaucgcaa auaucgaaaa cauuguggau gccgacauau ggaaggauaa agagcagcag     1740

aaguuuaucc aaucaaagau ugucgaccuc aucgacaauu accuggugga aaagaguaag     1800

uuagagauua ucaacugccu gcuaaaaaua uauaauaccg aagauaucga gggcagaaag     1860

guuuacuaua gcaaagaggc ugaaucccuc uucgaggcgg accugaggaa gaaacuugug     1920

ccauuuuaca aggcaaacac aauuucugaa cagcaggagc aggagaucau uuucaaggau     1980

uuguuuccgg uccugaucga acaacucaaa aagcaggagu ucauaaaaau ugugcggcug     2040

gacaagaaaa ucgaagccuu uuuaaagggg gagaaugaag agggacagau uuucuguaac     2100

uaucccgaga aguuuaaaaa gcuguaccac ccuucggaua ucgaaguauu caaaaagaaa     2160

auaaucaagg acgagugggg caaugaaaag guugugcuug guagcccauu gacuaccucc     2220

auuaaaaacc ccauggcuau gcgcgcccuc caccagcuga gaaagguccu aaauacacug     2280

auccucgaug ggcaaauuga cgagaaaacu aggauccaua uagaaauggc acgugagcug     2340

aacgaugcca auaagcggaa aggcauucag gacuuucaga aggagcgaga ggaacuuuau     2400

aagaucuacg aagcuaaaau caaggaguug uaccagguga aaaccggaaa ggaacuguua     2460

cacgucacag augaggaccu ccaaaaauuc gagugggccc uggaacagac caaggauggc     2520

agaauuauca cuaaggagga acuuauuaaa uauaagauau gggaggagca gaaccauauc     2580

ugcauuuaca cggggaaaac aaucucaauu gacaguuuuc ugggagguaa uccuaaguuc     2640

gacaucgaac acaccauacc aaggucuauc agccaagaua acuccuugau gaacaaaacu     2700

cucugugaca aggauuucaa ucgcucagug aaaggcaaga agaugcccgu ugagcugagc     2760

aacuauaaug aaauucuacc gcggaucgca cacuggaaaa agcaggcgga ggaacugacc     2820

agacagauug agacaaucuc uagguccaua aaaagugcug ccaccaaggu ggcaaaagac     2880

aagaacauuc gcaagcgaca uuaccucacu cugaaacggg auuauaucca ggggaaguac     2940

gaccguuuua cauggggaga gccuaaagua ggcuucaaga auagccaaau ccccgauacg     3000

gguauuauaa ccaaauaugc ccaggcuuac cuuaagucuu acuuuaagag agucgaaucc     3060

gugaaagggg gcgccguugc agaguucagg aaguuguggg gaauccagga aucauuuauu     3120

gacgagaacg gcuucaaaca cuuuaaggaa aaagaucgca guaagcauac ucaccauaca     3180

aucgacgcua uuaccaucgc cugcaugcca aaggauaaau augaccuguu agcgcacgcc     3240

uggcggcucg aggaugagca gaauaagaaa gcugcaaagg cccugauaga acaagcuaaa     3300

ccuuggaaga cauucaaaga ggacaucgaa aagauugagg ccgaaauccu ugugagccac     3360

uuuacucccg acaacgucaa gaaacaggca aagaaaauuc ugagagugag ggggaagaaa     3420

caguacguag cggagaucga gcgaaaugcc aacggaaagg cuauaccaaa gaaagaugcc     3480

ucggguaaga uuaucuauaa acuaaaugaa cgcggcaaca agauccccau ucuccagcaa     3540

ggggacacca uccggggauc ccugcaucag gauucuauau acggcgcaau uaaaaauccu     3600

uugaacacga augagaucag auaugugauu aggaaggacc uggagagcuu aaaggcuaac     3660

gauaucgaaa acauaguuga cgagaauguc aaagccaagg ugaaagaagc aguggccaac     3720

aagauucucc ugaucucaag uaauccacag cagaaaaaca aguucgcuga ggguaagaau     3780

gucuggauga acgaagagaa aaagaucccg auuaauaaag uucguaucua cgcgaacucc     3840

gugaagaauc ccauugaaau caaaaagcac ucuacccuua gcaaauccaa gcaugagcac     3900

aagcaaacag uguaugggca gaacgaugag aauuacgcca uggcacugua cgaauuggac     3960

ggcaaacgcg aguuugaacu cauaaacaau uucaaccugg cuaagcuacu gaaacaggga     4020

caaggcuauu acccucuuca caaggagaaa gaaauuaagg ggaagaaagu acucauccca     4080

aucgagaagc ggaauaacag agaugucgug cugaaaaggg gacagcaggu uguguucuau     4140

gacaagacug ucgaaaauuc aaaagauauu agcgaaauag uggacuuucg aggccggguu     4200

uacuugaucg agggucugac cauucagcgc caaaaggaca agaaaacagg gaaggugaac     4260

gaauauggaa ucauucaguu aagauacuuc aaagaggcca ggaagaguga agagaucucu     4320

aaagauuccu uuaagcccga cggcgaguuc aagauaaacg aaaauaaacc uacucggaag     4380

augaaccaua aucaguuuac cgccuucgua gaggguaucg auuuuaaagu ccucccaagc     4440

ggcaaguucc agaaaauuug a                                               4461


<210>  3406
<211>  4464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3406
augaagaacg ugcugggccu cgaccuuggg agcaauuccg ucggaugggc ccugguuaaa       60

accgaggaua acgguaaucc caucggcucu auuaagaugg ggucaagaau aaucccuaug      120

agucaggaca uuuugggcag cuucgaaaaa ggagauacag ugucccaaac ugcucagagg      180

acguuauacc gggguaagcg ccgacugauc gagcgucacg uacuaagaag ggaacggcuc      240

ugccgcgugc ugcauauuau gaacuuucuu ccagaccacu ucgauaaacu guuggggugg      300

gacaagaccg auaauaagac auauggaaaa uuuaucgacg auucugagcc gaagcucgca      360

uggagacaga acaaaaauga aaaggagaaa auggaguucg ucuuuaugga cucguuccau      420

gaaaugcugu cagauuuugc caagcaccag ccccaacuga uagcgaacgg caagaaagug      480

ccucucgacu ggacuaucua cuauuuaagg aagaaagcuc ugacccagcu uauuagcaag      540

gaggaauugg ccuggauccu gcuaaauuuc aacaaaaagc ggggcuacua ucagcuccgc      600

ggggaggagg aagaggaaca gccaacaaag aaggaggaau acaagguucu gaaagugauu      660

agugucgaug cagacgaggg acaaaagggu aauggcaucu gguauaacau acaucuugag      720

gacgggggaa uuuacaaacg aaaguccgau acaccccugu acgacugggu gggcaaaacu      780

uugcagcuca ucguaguuac aauuuaugaa aaggauggua agaccccuaa aaucuuugac      840

ucugggaagc agaauccaag cuacagacug cccaaagagg augacugggg auuaaucaag      900

aaaaggacgg aaucccagau ucaaucaagu ggcaagacug ugggcgccuu cauauaugau      960

aacauccuga gcaagccuga cgauaaaauu cgggggaauc ucguccguac caucgagcgc     1020

aaguacuaua aaaaggaacu uauagagauu cugcagcagc aagcuaaaua ccacgacgag     1080

uugcacaacc agaaccuacu ggaaucuugu gcaaaggagc ucuaugccca uaaugaugug     1140

agauacaacg aaguggucaa guccaauaug auccaucugc uuguugacga caucaucuuu     1200

uaccagaggc cacugaaauc aaagaaaagu uugauugcua acugcaagua ugagagcuac     1260

gaauaugugg auaaagagac aggagaggua aaggaaaugc cgauuaaaug uauagccaag     1320

ucuaaucccu acuaucagga guuccggcuc uggcaauuua uccacaaccu gcgauuaucc     1380

gacgugguca cucgcgaaga ugugaccgcg caguaccuga gcacaccuca ggacuauugc     1440

aaucuauuca ccuaccucaa cgauagaaag gagauuaaac aggacauccu gcuuaaggau     1500

uucuuuaaaa ucaagaaagu ucaaauugac aaggagaagg uguucccaau agaauggaau     1560

uacaucaaag augcaacgaa gacuuauccc uguaacgaga caagguacga auugcugucg     1620

gcccucaaac gggcugguau ggacaagcag uggcuggaug acaccccuaa uaugcaguau     1680

cgccuuuggc accuguugua cucugucgag gaacaggagg aguccgccaa agcacucaga     1740

aagcuguaug auaacgacaa uuuuguguca aguuucuuaa agauuaaacc auuuaagaaa     1800

ugcuacggcg cuuauagcga aaaggccauc aaaaggcugc uaacucucau gcguaugggg     1860

aaggcguggg acgagcaagc cauugaugca aagacacuga aauguaucca gcagauaauc     1920

accggaaaca uugacuccaa acuuaaagaa aagaucgaug acucucggua cguauugcac     1980

cauguuagcg auuuccaggg ccugcccacg uaccucgcuu cauaugugau uuacggucga     2040

cacagugagg ucaccgacau cagaugcugg gauacaccga aagaccuguu aaaguuuaua     2100

uguggguucc cucaacauuc ccugaggaau cccauugugg aacaggucau ccuugagacu     2160

uugcgcgugg uuuaugauau uuggaaagcc gagaagcaca ucgacgaaau acauguggag     2220

guagcucggg aaaugaagcu caccgcccag caaagaaacg aggcaaaccu gaggaaucua     2280

aacaaugaag ccacaaaccu gcgcaucaaa uaccuccugg aggagcuuaa gaaugauacu     2340

uuuauuaaag acguccgacc auugagcccu augcaccagg aaaagaugcg gaucuaugag     2400

caggcugugc ugggcaacuu aaauaaacag gauaaggacu acgacgauau uaucaagaua     2460

ucuaaaaagg aacgucccac cucagcggag cucauuagau auaaacugug gcuugagcaa     2520

aaguacugca guccauacac aggaaaagca aucagccugu ccaaguuguu caccacugcc     2580

uaucagaucg aacacguuau uccccagucu agguacuuug acaacucguu caauaacaag     2640

gugaucugug agagcgaagu caauaaagag aaggauaaaa ugcucggcua ugaauuuauu     2700

aaggggcgcg gaggugaccg gauauacugc acggcucaug gcaaagaggu gacaauccug     2760

aaggaggaag aguauaagca guucguaaac gaacacuacg cccauaauaa aagaaagagg     2820

gauaaccuac uggcagagaa uauuccugaa uccuucucaa gucgacaacu caacgacacc     2880

cgcuauauca cuaaaaccau uaugucucug cuuagcaacg ugguucggca gcagggggag     2940

gaggaagcca cagcuaagaa uuugcugcca uccucaggag ccgucaccga uaaauuaaag     3000

aacgacuggg gccucaagga uguguggaau acuaucguga caccgagauu ugagaggcug     3060

aacaaacuua gcgguacgaa ugacuucggg gaaaugaagg aggaaaacgg caaucguuac     3120

uuucagacca acguccccuc ugaguacgag aaaggauuca agaaaaagcg cauagaucac     3180

cggcaccaug caauggacgc gcugguuauc gcuugcacua caagaaauau ggugaacuau     3240

auuuccaaug ugaacgccaa uaguccuaaa caaagggaag accuacgaca gcuccugugu     3300

gauaagaacc ggaucauuaa uaagccaugg aacaccuuua cacaggacgc auugaaagcc     3360

cugaacgaua ucguagucag cuucaagaau aacgugcgca uaauuaauag agcuacuaac     3420

agguaccaac gguaugacaa aaauggcaag aaaaugaucu gcucucagaa ggggaacgau     3480

augugggcca uccgaaaguc caugcacaaa gagaccguuu uuggaagagu gaauuuaauu     3540

cgcaaggaag cacuccccau agcuaaagcc cuggacaaca ucucagcgau uuguaaugcc     3600

cagcuuaggg cuuacgucaa cgaucuggug gacaagcauu ucaauaaaaa gcaguugguu     3660

gcacacuuua agagucucga uuauaaaugg aaccgucaag acgugagcaa gguagagguc     3720

uggaucucgu ccgaugacaa aacgccuaug guggccgugc ggaagccacu ggauaccucu     3780

uucgacaaaa agaagauugc uagcaucaca gacacuggua uacaggccau ccuacugaau     3840

uaccuugagu caaaaggcgg ggaugcacuc gucgcguuug cccccgaagg cauucaggag     3900

augaacagua auaucgaacu guauaacaau ggaaaggcuc auaaaccgau uaagaacguu     3960

agauuguccg agacccuggg ugccaaauac cagguggggg aaaggggcgc aaagaccaaa     4020

aaguacguag uggcugacaa gggaacuaac uuauucuuug ccaucuauga ggaugaggaa     4080

ggcaaacgcu acuauuacuc ugucccucuc cgagaggcaa uagaaagaca aaagcagaaa     4140

cugagcccag ugcccgagua uaaugaaaag gggguugccc uuaaauucua ccuguccccu     4200

aacgacuugg uguauguccc caccgaggag gaacggacga ugggaacaug ccacauugau     4260

aagaccagga ucuacaagau ggugucaagc agugagaauc gcuguugcuu cguuccaucu     4320

acuaucgcua aagugauugc ggacaagguc gaacuccugu cccacaacaa aaucgagaag     4380

gccauaacag gcgaaagcau uaaagaggua uguuggaagc uaaaggugga ucgucugggu     4440

aauauccuua acguuauuaa auga                                            4464


<210>  3407
<211>  4464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3407
augaagaacg ugcugggccu cgaccuuggg agcaauuccg ucggaugggc ccugguuaaa       60

accgaggaua acgguaaucc caucggcucu auuaagaugg ggucaagaau aaucccuaug      120

agucaggaca uuuugggcag cuucgaaaaa ggagauacag ugucccaaac ugcucagagg      180

acguuauacc gggguaagcg ccgacugacc gagcgucacg uacuaagaag ggaacggcuc      240

ugccgcgugc ugcauaucau gaacuuucuu ccagaccacu ucgauaaacu guuggggugg      300

gacaagacag auaauaagac uuauggaaaa uuuauugacg auucugagcc gaagcucgca      360

uggagacaga acaaaaauga aaaggagaaa auggaguucg ucuuuaugga cucguuccau      420

gaaaugcugu cagauuuugc caagcaccag ccccaacuga ucgcgaacgg caagaaagug      480

ccucucgacu ggaccauaua cuauuuaagg aagaaagcuc ugacacagcu uaucagcaag      540

gaggaauugg ccuggauucu gcuaaauuuc aacaaaaagc ggggcuacua ucagcuccgc      600

ggggaggagg aagaggaaca gccaaccaaa aaggaggaau acaagguucu gaaagugauc      660

agugucgaug cagacgaggg acaaaagggu aauggcauuu gguauaacau ccaucuugag      720

gacgggggaa uauacaaacg aaaguccgau auuccccugu acgacugggu gggcaaaacu      780

uugcagcuca ucguaguuac aauuuaugaa aaggauggua agaccccuaa aaucuuugac      840

ucugggaagc agaauccaag cuacagacug cccaaagagg augacugggg auuaaucaag      900

aaaaggacgg aaucccagau ucaaucaagu ggcaagacug ugggcgccuu cauauaugau      960

aacauccuga gcaagccugc ugacaaaauu cgggggaauc ucguccguac caucgagcgc     1020

aaguacuaua aaaaggaacu uauagagauu cugcagcagc aagcaaaaua ccacgaugag     1080

uugcacaacc agcaucuacu ggaaucuugu gccaaggagc ucuaugcuca caacgacgug     1140

agauacaaug aaguggucaa guccaacaug auccaucugc uuguugauga caucaucuuu     1200

uaccagaggc cacugaaauc aaagaaaagu uugauugaca auugcaagua ugagagcuac     1260

gaauaugugg auaaagagac aggagaggua aaggaaaugc cgauuaaaug uauagccaag     1320

ucuaaccccu acuaucagga guuccggcuc uggcaauuua uccacaaucu gcgauuaucc     1380

gacgugguca cucgcgaaga ugugaccgcg caguaccuga gcacaccuca ggacuauugc     1440

aaccuauuca ccuaccucaa ugauagaaag gagauuaaac aggacauccu gcuuaaggau     1500

uucuuucaaa ucaaaaaggu ucagauugac aaagagaagg uguucccaau agaauggaac     1560

uacaucaagg augcaacgaa aacuuauccc uguaaugaga caaaguacga auugcugucg     1620

gcccucaaaa gggcugguau ggacaagcag uggcuggaug acaccccuaa caugcaguau     1680

cggcuuuggc accuguugua cucugucgag gaaaaagagg aguccgccaa ggcacuccgc     1740

aagcuguaug augacgacaa uuuuguguca aguuucuuaa aaauuaagcc auuugaaaaa     1800

ugcuacggcg cuuauagcga gaaggccauc aaaagacugc uaacucucau gaggaugggg     1860

aaggcguggg augaacaagc cauugacgca aagacacuga aauguaucca gcagauaauc     1920

accggaaaca uugauuccaa acuuaaagag aagaucgacg auucucguua cguauugcau     1980

cacguuagcg acuuccaggg ccugcccacg uaccucgcuu cauaugugau uuacggucgg     2040

cauagugaag ucaccgauau ccgaugcugg gacacaccga aagaucuguu aaaguuuaua     2100

uguggguucc cucaacacuc ccugagaaau cccauugugg agcaggucau ccuugagacu     2160

uugagggugg uuuaugacau uuggaaagcc gaaaagcaua ucgaugagau acacguggaa     2220

guagcucgcg agaugaagcu caccgcccag caacggaacg aagcaaaucu gagaaaccua     2280

aacaaugagg ccacaaaccu gaggaucaaa uaccuccugg aggaacuuaa gaaugacacu     2340

uuuauuaaag auguccgccc auugagcccu augcaccagg agaagaugcg aaucuaugaa     2400

caggcugugc ugggcaacuu aaauaaacag gacaaggacu acgaugacau uaucaagaua     2460

ucuaaaaagg agcggcccac cucagcggag cucauucguu auaaacugug gcuugaacaa     2520

aaguacugca guccauacac aggaaaagca aucagccugu ccaaguuguu caccacugcc     2580

uaucagaucg agcauguuau uccccagucu agauacuuug auaacucguu caauaacaag     2640

gugaucugug aaagcgaggu caauaaagaa aaggacaaaa ugcucggcua ugaguuuauu     2700

aaggggaggg gaggugaucg cauauacugc acggcucacg gcaaagaggu gacaauccug     2760

aaggaagagg aauauaagca guucguaaac gagcauuacg cccacaauaa acggaagaga     2820

gacaaccuac uggcagaaaa uauuccugag uccuucucaa guaggcaacu caacgauacc     2880

cgauauauca cuaaaaccau uaugucucug cuuagcaaug ugguucgcca gcagggggag     2940

gaagaggcca cagcuaagaa cuugcugcca uccucaggag ccgucaccga caaauuaaag     3000

aacgauuggg gccucaagga cguguggaau acuaucguga caccgcgguu ugaaagacug     3060

aacaaacuua gcgguacgaa ugauuucggg gagaugaagg aagagaacgg caauagguac     3120

uuucagacca acguccccuc ugaguacgaa aaaggauuca agaaaaagcg uauagaccac     3180

cgccaucacg caauggacgc gcugguuauc gcuugcacua cacggaauau ggugaacuau     3240

auuuccaaug ugaacgccaa uaguccuaaa caaagagagg aucuaaggca gcuccugugu     3300

gacaagaacc gaaucauuaa uaagccaugg aacaccuuua cacaggaugc auugaaagcc     3360

cugaaugaca ucguagucag cuucaagaac aacgugcgga uaauuaaucg cgcuacuaac     3420

agauaccaaa gguaugauaa aaauggcaag aaaaugaucu gcucucagaa ggggaacgac     3480

augugggcca uccggaaguc caugcauaaa gaaaccguuu uuggacgagu gaauuuaauu     3540

agaaaggagg cacuccccau agcuaaagcc cuggauaaca ucucagcgau uuguaaugcc     3600

cagcuucgcg cuuacgucaa cgaccuggug gauaagcacu ucaauaaaaa gcaguugguu     3660

gcacauuuua agagucucga cuauaaaugg aacaggcaag augugagcaa gguagagguc     3720

uggaucucgu ccgacgauaa aacgccuaug guggccgugc guaagccacu ggacaccucu     3780

uucgacaaaa agaagauugc uagcaucaca gauacuggua uacaggccau ccuacugaau     3840

uaccuugaau caaaaggcgg ggacgcacuc gucgcguuug cccccgaggg cauucaggaa     3900

augaacagua auaucgagcu guauaacaau ggaaaggcuc acaaaccgau uaagaacguu     3960

cgguuguccg aaacccuggg ugccaaauac cagguggggg agagaggcgc aaagacaaag     4020

aaguacguag uggcugauaa gggaacuaau uuauucuuug ccaucuauga ggacgaagag     4080

ggcaaaaggu acuauuacuc ugucccucuc cgcgaagcaa uagagcgaca aaagcagaaa     4140

cugagcccag ugcccgaaua uaacgagaag gggguugccc uuaaauucua ccuguccccu     4200

aacgauuugg uguauguccc caccgaggaa gagagaacga ugggaacaug ccacauugac     4260

aagacccgga ucuacaagau ggugucaagc agugaaaaua gguguugcuu cguuccaucu     4320

acuaucgcua aagugauugc ggauaagguc gagcuccugu cccauaacaa aaucgaaaag     4380

gccauaacag gcgagagcau uaaagaggua uguuggaagc uaaaggugga ccgccugggu     4440

aauauccuua acguuauuaa auga                                            4464


<210>  3408
<211>  4464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3408
augaagaaca uccugggccu cgaccuuggg agcaauuccg ugggaugggc ccuggucaaa       60

guugaggaaa acgguaaucc cauuggcucu auaaagaugg ggucaagaau cauuccuaug      120

agucaggaua ucuugggauc auucgagaaa ggcgacaccg ugucccaaac agcucagagg      180

acuuuauacc gggguaagcg ccgacugauu gaacgucacg uacuaagaag ggagcggcuc      240

ugccgcgugc ugcauaucau gaacuuucuu ccagcacacu ucgauagacu guuggggugg      300

gacaaaacgg auaauaagac cuauggaaag uuuauagacg auucugaacc gaaacucgcc      360

uggaggcaga acaagaauga gaugggcaaa auggaguucg ucuuuaugga cucguuccau      420

gaaaugcugu cagauuuugc gaagcaccag ccccaacuga ucgcuaacgg caaaaaggug      480

ccucucgacu ggacaauuua cuauuuacgg aagaaagccc ugacucagcu uaucagcaag      540

gaggaauugg cauggauucu gcuaaauuuc aacaaaaagc gcggguacua ucagcuccga      600

ggagaggagg aagaggaaca gccaaccaaa aaggaggaau acaaaguucu gaaggugauc      660

agugucgaug ccgacgaggg ucaaaagggc aaugggauau gguauaacau ucaucuugag      720

gauggaggca ucuacaaaaa gaaauccgac auuccccugu acgacugggu ggguaagaca      780

uugcagcuca ucguaguuac cacuuaugaa aaagauggga agacaccuaa gaucuuugac      840

ucuggaaaac agaauccaag cuacagacug cccaaggagg augacugggg cuuagugaaa      900

aagaggaccg aaucccagcu gcaaucaagu ggcaaaacgg ucggggcuuu cauuuaugau      960

aacauacuca gcaagccuga cgauaagauc cggggaaauc uugugcguac uauugagcgc     1020

aaauacuaua agaaagaacu gaucgagaua uugcagcagc aagcaaagua ccacgacgag     1080

cuacacaacc agaaccugcu cgaaucuugu gccaaagagc uguaugcuca uaaugaugug     1140

cacuacaacg aagucauuaa guccaauaug aucaaccuuc ugguuaauga caucaucuuu     1200

uaccagagac cauugaaguc aaagaaaagc cucauugcca acugcaaaua ugagaguuac     1260

gaauaugugg auaaggagac cggugagauu aaagaaaugc cgauaaagug uaucgcgaaa     1320

ucuaaucccu acuaucagga guucaggcug uggcaauuua uucauaacuu acggcugucc     1380

gacguaguga cacgagaaga cgucacugca caguaccuaa gcaccccuca ggauuauugc     1440

aaucucuuca cauaccugaa cgaccgcaag gagaucaagc aggauauccu uuugaaagac     1500

uucuuuaaga uuaaaaaggu gcaaauagau aaagagaagg uuuucccaau cgaauggaau     1560

uacauuaagg acgccaccaa aucguauccc uguaacgaga cgagauacga acugcucucu     1620

gcucugaaga gggccggcau ggauaaacag uggcuugacg auacuccuaa uaugcaguau     1680

cggcuguggc acuugcucua cuccguggag gaaaaggagg agucagcaaa agcucugcgc     1740

aaguuauaug acgaugacaa guuugucaca aguuuccuga aaaucaagcc auuuaacaaa     1800

ugcuacgggg ccuauagcga aaaggcgauu aaaagacuac ucacccugau gaggauggga     1860

aaggccugga augagcaggc aaucgaugcu aagacucuua aauguauaca acagaucauu     1920

acagacaacg uggacuccaa guugaaagaa aagaucgaug acucucguua cguacugcac     1980

cauguuagcg auuuccaggg ccuccccacc uaccuggccu cauaugugau uuacggucgg     2040

cacagugagg ucacggacau ccagugcugg gauuccccgg aagacuuacu gaaauuuaua     2100

cgagauuuca agcaacauag ccuuagaaac ccuauugugg agcaggucau cuuggagacc     2160

cucagggugg uuuaugacau uuggaaagcu gaaaagcaca ucgaugagau acauguggaa     2220

guagcccgcg agaugaagcu gacagcacag caacggaaug aagccaaccu aagaaaucug     2280

aacaaugagg cuacuaaccu caggaucaaa uaccugcuug aggaauugaa gaaugacacc     2340

uuuauuaaag auguccgccc ccugucucca auccaccagg agaagaugcg aauuuaugaa     2400

caggcggugu uagggaaccu caauaaacag gacaaggauu acgacgacau cauaaagauu     2460

agcaaaaagg agcacccuac aaacgcagag cugauccggu auaaacuuug gcuggaacaa     2520

cguuacugua gucccuacac uggcaaggcc aucagcuugu ccaaacucuu caccacagcu     2580

uaugagauug aacauaucau uccacagucu agauacuuug auaauucguu caacaauaag     2640

guuauaugcg aggccgaagu gaacaaggca aaaggaaaua ugcugggcua ugaguuuauc     2700

aagaaacagg gcggacagaa gauuuacugu acccaacuag guaaagccgu cacuauccug     2760

aaggaggagg cuuauaagca guucgugaac gaacacuacg cccauaacaa aaggaagcgc     2820

gacaaucucc uggcagaaaa cauucccgag agcuucuccu cacggcagcu uaaugauacg     2880

agauauauca caaaaaccau aaugaguuug cugucuaacg uagugaggca gcaaagcgaa     2940

gaggaggcga cugcuaagaa uuuacucccu uccucaggca gcguuaccga ccgacugaaa     3000

aacgauuggg ggcuuaagga cgucuggaau acaaucguga ccccacgcuu ugaacggcug     3060

aacaagcuau cuggaacuaa ugauuucggc gagaugaaag aagagaacgg uaauagauac     3120

uuucagacaa acgugccgcu cgaauacgag aagggguuca aaaagaaaag gauugaccac     3180

cgucaccaug ccauggaugc acuggucauc gccugcacga cccgcaauau gguuaacuau     3240

auuuccaaug cuaacgccaa cagucccaag cagcgggagg acuugagaca acuguuaugc     3300

gauaaaaaua ggaucauaaa caagccuugg gacacuuuua cacaggacgc acucaaggcu     3360

cugaaugaua uuguggugag cuucaaaaac aauguacgaa ucaucaaccg ggccaccaau     3420

cgcuaccaga gauaugacaa gaacggcaaa aagauuauau uuucucagaa aggagaugac     3480

augugggcga ucaggaaguc caugcacaag gaaacagucu ucggccgggu gaaucuuauu     3540

cgaaaagagg uucugccaau cgccaaggcu uuggauaaca uuucagcaau cuguaaugcc     3600

caacucagag cuuacgugaa cgaccugguc gauaaacauu uuaauaagaa acagcuagug     3660

gcccacuuca agagucugaa cuauaagugg aaucgccagg acguuagcaa aguggaagua     3720

uggauaucgu ccgauaacaa gacucccaug gucgcaguga ggaaaccucu ugacaccucu     3780

uuugauaaga aaaagaucgc gagcauuacg gacaccggga uccagacaau ucuccugaau     3840

uacuuggagu caaagggagg ugaugccagu guggcuuucg ccccagaggg cauccaagaa     3900

augaacucca acauagagcu guauaauaac gggaaagcac auaagcccau uaaaaauguc     3960

cguuuaucug aaccgcucgg agcuaaguac cagguuggcg agcggggugc caaaacuaag     4020

aaauacgugg aagcagacaa ggggaccaac cuguuuuucg ccaucuauga ggacgaggaa     4080

ggcaagagau acuauuacag cguaccucuu agggaggcua ucgaacgcca gaaacagaag     4140

cuguccccag ugcccgagua uaaugaaaaa ggagucgcgu ugaaguucua ccucucaccu     4200

aacgaucugg uguauguucc cacagaggag gaacgaacua uggggaccug ccacauugac     4260

aaagccagaa ucuacaagau ggugagcagu acgggguguu cuugcuacuu ugucccaucc     4320

acauucgcaa aggugauagu ugauaaagug gagcuacaaa gccugaauaa gauggaaaaa     4380

gcuauuaccg acgaguguau caaggaaguc ugcuggaaac uuaagguaga ucggcucgga     4440

aacauuauca augugauaaa guga                                            4464


<210>  3409
<211>  4485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3409
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugggc ccugauagau       60

aacgaguccc acagaaucau uaaaucuggu ucaaggauca uucccaugga cgcugcaaca      120

augggcgauu acgaaaaggg gaauuugaaa agugccgacu ggaagcggac uagcuuccgc      180

ggaaugcgac guuuauauga gagagcgaaa cugaggcggg aacgccuacu cagagugcug      240

aacauccuug gcuuucugcc ugaggaguuc aggcggcaga uagauuuuga acaucaccca      300

ggucaauuca ucgacgggaa ggagccguug auugcuuacc aucacgauga aaauggacag      360

cgcgucuuuu ccuauauguc ugccuucgag gaaaugcuca cggacuuucg agcaacccag      420

ucggagcugg uuagagaugg caggcgggug cccuacgacu ggacaaucua uuaccugcgu      480

cauaaggccc ucacucagcc uguaaccaaa gaggaauuag cuuggauuau ccugaacuuc      540

aauacaaagc gcggcuauua ccaacuuaga ucagagagcg aagaggugac cacuaacaaa      600

aaugaagagu acgcagaguu gaaggucgug aguguugaaa agacagggga ggauaagaag      660

agggccggau auuacuggua uaccauaacg uacgacaacg aagcugagca gaaaauuacu      720

uccaccaucc caccccggca gauuggugau aagguggaac ugaucgucac aacugcccga      780

gacaaagcgg gcgagaucaa gaaaucugug cgcagcccua aggaggacga uuggacccua      840

augaaaaaga gaacagaaca caauauugag gaagaggaau gcaccguagg guccuauaua      900

uaccagcaca uccucgcaga cccaacgguu aaggugaggg gaaaacuggu ccauacuauu      960

gagcggaagu uuuauaaaaa ggagcuuaaa caaauccuga acaagcagaa ggaguucaua     1020

cccgaauugc aggaugccuc acucuacgag gcuuguauua aagaacugua ccgcaauaac     1080

gaggcccaca uccagaguau cgcaaauaag gacuuuacag auuuauucgu gaacgacauu     1140

aucuuuuauc aaagaccucu gaaaagcaag aaaucuacca uuuccaacug cccauacgaa     1200

ucauauuacu auaagaauaa ggagacuggc gaacucauaa ggaaaccgau caaguguauu     1260

gcuaaaagca acccccuuua ccaggaguuc cgucuguggc aguucgugca uaauuugcgg     1320

aucuauaaga aaagugaaga ggucucuggu cgacuacaaa cagauguuga cgugaccgau     1380

agauuucuga cgaccccuga cgauuacgcc aaacucuucu ccuggcugaa cgacaggaag     1440

gagaucaaac agaaggaacu ucugcgcuac gaggcguuug gguugagcaa aucguaugga     1500

gacuaccggu ggaauuucgu agaagauaag ccauaucccu gcaacgagac acuccacgaa     1560

auuaauagaa ggaugaaauc uauacugucc gacaagaaau uaucaaguga ggccuaccag     1620

cagcuguggc acauccuaua uagcauugau gacccuaucg agauucgcaa ggcacucacu     1680

uccuuugcuc aaaaccagga aaucaauucu gaugacuucg ugaacgccuu uuaccaguau     1740

gucccagaga agaccaaaag cuacggcgcu uacucagaaa aggccauaaa gaagcugcuu     1800

cccuugaugc gacggggcag uuauugggca aucgggaaua uugaugagaa aacacaucgu     1860

agacuggaca acaucauuaa uggaguggcc gaugacucca ucagcacuug uaccagggaa     1920

aagcucaaca aucugacaga uauaaccaug ugccagggua ugccgcuuug gcaggcuugu     1980

uacgcgguuu augaccgcca cucugaggca gccgauacua cgaaguggga gcaaccugac     2040

gacauugaua cauaccuguc aaccgaauug aaacagcaua gucuccggaa ccccaucgug     2100

gagacuguca uuagcgaaac ccugagagug guaagggaca ucuggaaggc uuauggccga     2160

ccagaggaga uacacguuga auuagggcgc aaccugaaac aggaugccaa gucccggaaa     2220

caagacgcaa ucagaauggc cgagaaugaa cagacaaacc uaaggauucg ugcucuccug     2280

caggagcuuu cuaauccuga augcggagug gagaacgucc gcccccauuc gccaagccag     2340

auggaguugc ugaagaucua cgaaaaugag gugcuccaau ccaccgauga cauucccgaa     2400

gagaucucaa acauaauuaa ggaucugggc aaaacugaca guaagaaacg gccuucuuua     2460

agcgauauca caaaguauag acuguggcuu gaacagaaau accagucccc auacacgggu     2520

cacaucauuc cguugucaaa acucuucacc cccgccuauc aaaucgagca cgucauuccu     2580

cagagcaggu acuuugacga uucuauaucc aauaagguga ucugugaggc agaaguuaac     2640

aaagagaagg accgaaugcu gggguaugag uucauuauca auaaaggcgg aaguauugug     2700

aagggcagcu uugggcagca guucaaaauc uuugauaagg uacaauacga agacuucguc     2760

aaacagcggu augcgcauaa caagacuaag augaaaaagc uacugaugga cgauauacca     2820

gacggauuca uccagcgcca gcucaaugau ucuagauaca uguccaaaaa gauuaucuca     2880

auucugagua acaucgugag ggaagacggu gagaaagagg cuacaagcaa gcacauaauu     2940

guuaccucgg gcgccaucac agaucggcuu aagaaagaau gggggaucaa ggacgugugg     3000

aaugauauua uagcaccccg auuuaaaaga uugaacaagc ugacugacuc caauaacuau     3060

ggaauggaga ccgaaaaaga gggccgccaa uacuuccaga ucaacguccc uacggaguua     3120

ucuaccgguc ucauuaagaa gaggaucgau caucgucacc acgccaugga cgcuauuauc     3180

auagccugca caacucggaa ucaugugaac uuucugaaua acagcaccgc agcuucagau     3240

cagaaagaca ucagaaggga ccuucagcac acacugugua cuaagaccaa aacggauagu     3300

aaugggaacu acguauggcg cuucaauaag ccaugggaca cauuuaccca agauacucga     3360

caggaauugg agggcauuau cguguccuuc aaacagaacc ucagaauuau caauaagaug     3420

ucuaacuauu accaacauua uguugacgga cggaagacaa uaagcaggca gaauaaaggc     3480

gauggguggg ccauucgcaa gucccugcac aaagcgaccg ucucaggaaa ggugaaccua     3540

aaacguagca agacuguggg uuuuaaggaa gcccugaaug acuggagugc uaucaaagau     3600

aagaccucua aagcaauccu ccagaagauu aucgcccagu acaaaaacua ugaccccaag     3660

acaauauuca aauacuuuaa ggauagaaau uucaaggucg agggcaaaga cuuuuccaag     3720

cuggaaguuu auuacaacac gagcgauaaa gagccgaugu cggcucaucg ggugaacauu     3780

gacaccacuu ucgaugagaa ggaaaucagg ucaauuacag acaccgggau ccagaaaaua     3840

augcuuaagc acuugcacac auacgacgau gcccaaggaa agacucaucc ugaggcagcg     3900

uuuucuccag aaggcauuga gaaaaugaau cagaacaucc gcaugcugaa uggugggaag     3960

gaccacaaac ccaucuucaa ggugcgaaaa gccgaaauuu uaggcaugaa guucgcugua     4020

ggagagagag gccauaagga uaaaaagugg gucgaggccg acaaagggac caaccucuuu     4080

uucgcaaucu augaaaguga ggaaaauaag cgguccuaca gcucuauucc ucugaacgag     4140

gcuauagaua ggcuuaaacg cggagaaaau aucgccgacg agagagauca gaacggcaau     4200

aagcugcuau uugugcucag ccccauggac cugguuuaug ugccagaucc ugacgaugca     4260

cacauugacg aggucaaggg uaacaauaac aucuucaaaa ugguguccug cacggggaca     4320

gaaugcuacu uuguucccga gaccuaugcc aaugugaucu guaagggaaa agaauuggag     4380

ucaaagaaca aaguagagag uuacgauggc auuaauauaa agaaaagcug cuauaagauc     4440

acugucgaca ggcuggguaa ggugcggaaa auuuucaagg gcuga                     4485


<210>  3410
<211>  4491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3410
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc caaagugugc       60

gucgaugaca augguaacua ccugagagau auaaaguugg gcuccaggau cauucccaug      120

ucucaggacg uuuuagggaa uuucgauaaa ggagugacug agucacaaac gaguguacgg      180

accggcuuuc gcggugugcg acguaucaga gaaaggagcc ugcuacggcg cgagagacuc      240

cacagggucc ugcaugugcu uggguaucug ccuccacacu acgacuccgc uauuggaugg      300

aacaagaaug aaccgucuac auauggcaaa uucuugaacc auggcgagcc caagcucgca      360

ugggaacaca aggaggaugg gucgaugcgg uuucuguuca uggagucauu ucaugaaaug      420

auggccgacu ucgcgaaaag ccagccuagu cugguuuccg auggaaagaa aaucccacuc      480

gacuggacua uauacuauuu acgcaagaaa gcucugucuc agcccauccg aaaggaggaa      540

cuugccuggu ugcugcuaaa uuuuaaccag aagagagguu acuaucaacu caggggcgag      600

gaggaugacg auaauccuac caaaugugaa gaguacuacg aacugaaggu ggucgggauu      660

gaggcagacg aagagggaaa gaacggcaau acaugguaua acgugaaacu ugagaauggu      720

uggaucuacc ggcgucagag caaaauucca cuggacgauu ggaucgggaa gguacgcgag      780

uucauaguua ccacugaaua ugaaaaagac ggaauuacau ugaagaagga uaaaaacggc      840

gacgugaaga gauccuuuag gucacccagu gaggaugacu ggggccuccg gaaaaagcga      900

accgaaaaac uguuagauag cucuaagaug acggucgggg ccuucgugua cgaccaucug      960

cucgcugagc cuuccgauaa gauccgcgga aaauuuauua gaacuaucga gaggaaguau     1020

uacaaaaagg aacuuuaugc aauccugugc gagcagucaa aauaccacga agaguugcgg     1080

agcggugacu accuaaauaa gugugccgag gaacuguaua agaacaacca gccacaccgc     1140

aauagucucc ugaccaaaga ucuugcuuac cuguugcucg aggaccugau uuucuaucaa     1200

agaccguuaa agacuaaaaa gucucugaua gccgaaugcc ccuacgaggc guauaaauac     1260

guggacaagg aaacaggcga gaucaagagg caggggauua aauguaucgc aaaguccaac     1320

ccuuauuacc aggaguuucg ucuauggcag uucauaagca aucuccggcu guucaacaaa     1380

gccgaugaca aggaagucac cgcugauuac cuuccaacac aagacgauua uguucgauug     1440

uuugccuacc ugaaugacag aaaagagauu aaccaggaaa cccuccugaa ggaguucuuu     1500

aaacuuaaga agaucacggu aaauucggaa aaaguguucc ccaucaggug gaacuauauu     1560

gaggauaagg agaaaaagua cccuugcaau gaaacucgcc acgagcuguu gcucgcacug     1620

gaccgggcua gaaucgauca uucuugguua gaaaaaccag gacaggagua uaggcugugg     1680

caccuacucu acuccgugga aucaaaggag gagacaaagg aagcccugcg caaacuuaag     1740

gacgaugacg cguuugucga gaguuucuug aaagugaagc ccuuuaaacg agaauauggc     1800

gccuacagcg agaaggcaau uaagaaacug cucgcuguua ugcggauggg uucccugugg     1860

cgugaaaccg auauauguga cacuacaaag gggaacauca ccaaaauuuu acaaggcgag     1920

aucgacgaga agcugaaaga aaagaucucu gccagcgugu caaguuacaa ucagcuuucc     1980

gauuuccagg gauugccggu cuggcucgcu ugcuaugugg uauacggcag acacagcgag     2040

gccgcagaaa uucagcauug gaagacgccu gagucucugc uagccuauau aaacggguuu     2100

aaacaacacu cacugaggaa ucccaucguu gagcagugua uucucgaaac ccugcgcaca     2160

gugcaugaca ucuggaagga ggucggacac auugaugaaa uccaugugga gcuuggucgg     2220

aacaugaaaa guacugcuga ccagagagcg aggauaagcg aaaaugucuu gcgaaacgag     2280

aacaccaauc ugcgcaucaa guccuuacuc cuggagcuua aaaacgaucc aaauauuaag     2340

gacgugcggc cuuacucucc caugcagcaa gaaaaacuga gaaucuauga ggaaggcguu     2400

uugcaggagc ucacaaagga ugacaaggau uucacugaaa uuaccaaaau cucgacaaug     2460

gcacagccaa gcgccaagga gcugcagagg uacaaacuau ggcuggagca aaaguaucgu     2520

ucccccuaca ccgggaaauc aauaagucuc acuaagcugu uuacgucugc uuaccagauu     2580

gaacacguga ucccucagag ccgcuauuuc gacgauuccu uuucaaacaa gguaauuugc     2640

gagagcgaag ucaauucucu uaaagacaac auguugggau acgaguucau caagaaucac     2700

ggcggugaaa uaauccauug uacaaugcug ggggauguga aaguuuuauc cgagagugag     2760

uauaaggccu ucgugaacga ccacuacgca cauaauaaac ggaagagaga aaaccuccug     2820

cuugaggaca uuccacagga auuucugaau aggcaaauga acgauagccg auauaucucu     2880

aaggucguga aagcccuacu cuccaauauu guacgggcug agggcgagga agaggccacc     2940

ucaaaguucg ugguuaguug cagcggaggc aucacugacc gccugaaaaa ggauuggggg     3000

uugaacgacg ucuggaauaa aauaguguac ccgagauuug aaaggcugaa ccgguuaacc     3060

ggaacagagg cauucgguca gugggaaaac aaggauggca agcgaguguu ucagaccgcg     3120

gucccccucg agcugcaaag aggguucaau aaaaagcgca uugaccacag gcaccaugcu     3180

auggaugccc uuguuaucgc augcacuucg cguaacauca uuaauuaucu gaacaaugag     3240

uccgccaaca auccuaaaaa gcgggaagac uugagacaga aacucuguga uaagaacagg     3300

acaauccgca agccauggga gacguuuacc caggacgcua cugccgcacu ggaagaugug     3360

auugugucuu ucaaaaauua cguacgaaua aucaacaagg cuacaaauua cuaugagaga     3420

uacgacgucg aaggcaaaaa ggugauugcc gagcagcggg gagacgauau gugggcgauc     3480

aggaaaccca ugcacgaggc caccuauuac ggucgcguua accuacguag aaagaaaaag     3540

gugaaucugc gggucgcucu ugaaaacauu agcucaaucu gcgacaauaa gcucaggaua     3600

uauaucaacg ggcuggugga gaaaggcuuu aacacaaagc aaauuuugga uaaauucaag     3660

aauacuaacg accauuuuag uaugcagaaa aucgauaagg uugaaauuug gcaguucacc     3720

aaugaggaag agaagcuggu ggcaacgcgc aaaccuuuag acaccuccuu ugauacaaag     3780

aaaaucgccu cuauaacuga caccggcauu cagacaaucc uccugaacua ccuugagacu     3840

aagggagggg auagcaccau cgcuuucacg ccagaaggaa uuguagagau gaaucaaaac     3900

auauccaaau auaauaacgg caaggaacac augcccaucc ugaagguccg auuguacaca     3960

aaacucggua agaaaauucc gguggggcag accggaugua aggccaaaca guucgugauc     4020

gcagaggcgg gcacuaaucu guacuuugcc auuuaugagg acgaagaggg uaagagauca     4080

uaccggacaa ucccucuaaa cgaagucguu gagaggcuga agcagggccu uagcccagug     4140

cccgagaaaa augauaagga cguacaucuc aaauucuauc ugaguccuaa cgauuuggug     4200

uacgucccca uagaagacga gcacgaaucu uuagcuaagg accacaucua uaaauuugug     4260

gauuccagcg ggaccacugc caauuucauu ccacaucgcu cggcaaacgu uaucuuugag     4320

cugaauaaga aagacgcuga aaaguucugc ucaggaucua uuauccaaaa cgaguacggc     4380

guggggaguu cccagagcaa gaaucagaaa gccauaaccg gagaaaugau uaaggcaguc     4440

uguagaaaac ucgaggugga uaggcugggc aacguuauca aggugaucug a              4491


<210>  3411
<211>  4497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3411
augaagcaca uccugggccu cgaccuuggg accaacagca uuggaugggc cgugauaaga       60

gcucaucagg augcagacgg ucacacaacu cugcaagaua ucuuggccgc gggcuccagg      120

auuaucccca uggacgcugc cacguuaacc gauuucgaca aagggaaugc acggucugga      180

acagccgagc gcacuucauu ucgaaguaug cguagaaggc gggaacgcca gcugcuaaga      240

agggagcggc uccaucgcgu ccugaugcuu cugggcuucu ugccucagca cuacgcuaac      300

agccucaccc gauaugguga auuugcagau aauagagagu gcaagcugcc auggguuaaa      360

gacgaauccg augggaagua ccauuuccug uuucaggacg ccuuccacga gaugugugag      420

gauuuuaggc aacucgcucc gcagugggug gccgacggaa agaagguacc cuaugauugg      480

acauuauacu aucugcggug caaggcgcuu ucucguaaaa uuucgaagga agaguuggca      540

uggauccugc uaucauucaa ccagaaacgc ggcuacuauc aggccagaga cgaagaggac      600

gaggugacca cuaauaagcu cgaagaguac uacgcuggca aagucgugag gguugaugac      660

acaggggaac ggaagggaaa gaacguguau uacgauauaa cccuggagaa ugguuggguc      720

uaucgauacg ccgcacgcau cuauccugac ugggugggcc aaguaaaaga auuuauuguu      780

acgacuaccc uuaacccaga ugggacaccc aagacugaca aagcuggaga ggugaagaga      840

ucauucagga ugccuaaaga ugacgauugg acccugauca agaaaaagaa ggagcgggac      900

auugaucgca gucaggaaac aaucggccag uacauauauc acgccuugcu caaagaccca      960

ucccagaaga uuagagguaa acugaucggg accgucgaga ggaaguacua caaagacgaa     1020

uuacgucgga uucuggaucg acaaauccag uuucaucccg agcucagaga cagggaacuu     1080

uauuacgcgu gucugcagga guuguauccu ucuaaugugg ccuuccgcaa cacgcuagug     1140

aaucgggauu uuacuuaccu guuccucgac gauauccugu ucuaucaaag accacuuaag     1200

agcaagaaau cccagauuuc agacugcccg uacgaggaac acuuuuauac agauaaggag     1260

accggagaaa ggauaagugu ccccguuaaa uguaucgcag ugagccaucc ucuguaccag     1320

gaguaccgcu uguggcaguu cauucaaaac cuccgaaucu auugccggga agcucguaua     1380

gacggcaauc ugaugauuga uugggaggua acuaacgagu uacugcagac agaagaggac     1440

aagaccgccc uauuugauug gcucaaugac aaagaaucug ugaagcagcc agagcugguc     1500

gcuuuccuuu ugggcgggaa ggccaacaaa aagcagauca aagcauacag auggaacuau     1560

auggaugacg acacgaaggg auaccccugu aaugagacca gggccucccu gcucaaauau     1620

cugcgcgaag cucaaacaga uagcagcuuu cuuacucggg agagagaaga ggcgcugugg     1680

cacaucuugu acaguauuuc ugacaagggu gaacucaagu ccgcacuggg caaauucgcc     1740

gugaagcacg gguuaaacga gagguuuguu accguguuca gcaaauuucc uccauucaag     1800

aaagauuaug gaucguacuc ugcuaaggcc cugaaaaagc uacucccccu gaugcgaaug     1860

ggccaucacu ggcaggaggc auccuuuauc ccgucaacac gccagcggau ggaaaaguuc     1920

auuaauggug aguacgacga uaguauagac aaaagaacuu augaaaaacu ugagagcuug     1980

accgaagucu cccaguacag ggggcugccu ggcuggcucg ccugcuaugu gguauacgau     2040

cgucauucug agggacgcga caucgagcgg uggacaagcc ccucagauau ugacaguuau     2100

cuggcucaau uuaaacagca cuccuuacau aacccaaucg uugaaagagu gguccuggag     2160

acccuuagga cugugcgaga uaucuggaag caggucgacc acauugauga aauacacauc     2220

gaguugggcc ggaauaugaa acucccugcc gacaagcgca gacagaugac ggaacaagca     2280

uggaagaacg agcagacaaa ucugaggauu aaagcgcuac ugcucgaguu cgcucggccc     2340

ggguacgaca ucgaaaacgu gcgaccauau agccccuacc agcaagauca gcugagaauu     2400

uacgaggaag ccguucuugg agcaaaugag uguccugacg auaucucugc cauauugaag     2460

aaauucaacg gucugaaucc accgaccuca gcugaggugu uacgcuauaa gugcuggcuc     2520

gaacagaaau acaggagucc cuauacuggc cguaccaucc cucugagcaa acuuuuuaca     2580

uccgccuacg agauugaaca uaucauucca aagcagcggu auuucgacga uucuuuuucg     2640

aacaaaguaa ucugugagag cgaagucaau aagaaaaagg gggcaaugcu gggauacgag     2700

uucauaaaau ccuaucacgg cgcuucaauu accuugaaca gaggugggac ugccaguauc     2760

uuuucugugg aggcguacga aaauuucguu aaggagcauu acgcccacaa caggggcaag     2820

augaaaaagc uccugaugga cgaugugccc gacagcuuua uuucccgcca acuaaacgau     2880

ucacgauaua ucagcaaacu gaugaagucu auacucucca aucuggucag agaagaggga     2940

gaaaaagagg cuaucaguaa gaaccuuauu gugugcacag gcgggaucac ggaccgguug     3000

aaaaaggauu gggguauuca cgacguaugg aaugacauca uauucccuag guuuaagcgc     3060

cuggaugaga uggacagcuc ucauaccuac auuacuuuaa acacacaccg ucauccaauc     3120

cccuccaugc cgcucgaguu ccagagagga uuuaguaaaa agcggaucga ucacaggcau     3180

cacgcaaugg acgccauugu gaucgcuugc gccacccgca auauuguuaa cuaucugaau     3240

aacgaaucag cacgacacga ugcgaaaaca agcagaaugg accuucagcg gcugcuaugu     3300

acugccaaua ggaagcucau agauaaaccu uggaagaccu ucacgcagga uaccugcaag     3360

guccuggaag acaugaucgu gucguuucaa cgcacauuga gaauuaucaa caaaacuaag     3420

aauuacuauc ugagguucga ggacggcaaa aagcggcgug uguuacagac caaaggggau     3480

aacugggcua uucgcaagcc acuccauaag gaaacauucu acggagagau caaucugcga     3540

agaauagaga cucuuucccu gaacaaagcc uuggaaagga uccccgacau ugucgagcgg     3600

gaucuccgca agaaacuguc ugaacuacug agccuuggcu auaaugcaaa gagaaucaaa     3660

gguuacuuug aggagcacaa ggaagagugg ucagacguua accucaguaa gauuaaggcu     3720

uacuauuuca ccaagcagaa aaacaagagg uacuuugccg cacggacgga acuggacaca     3780

uccuucaaua aagauaagau cgagaacaag auagccgaca ccgcuauuca gaaaaucuug     3840

cugcaacauu uagaagcgua ugggaaugau guggagcagg ccuuuucucc ugagggcauc     3900

gacacuauga accgaaauau uagacagcuc aacggaggcc gcagccacca acccauauac     3960

aaggugaggg uauaugaaga ggcaacaaaa uucgcugucg ggcggcgugg aaauaagucc     4020

accaaauuug uggaagccgc caagggcacu aaccuguucu uugcuaucua cgagauuuca     4080

cagguugaug guaaugggaa cagacagacc agcaggacau ucaguucuau cccacuuaau     4140

guggcagucg aacgccugaa acagggauug ccucccgugc cagaggacga ggaaggcaac     4200

gccccgaagu auguucucuc cccuaaugau cugguguacc uacccacgga gaaggaacug     4260

aaacggcgag agauugcgau gccaaucgac aagagcagaa uauacaaaau gguaucgugu     4320

accgguaagg auugcuuuuu cauccccgcu aacacugccg ucccuauugu ggacagguau     4380

gaauacucau cuaugaauaa aaucggccgc gcacuuacag gggagaugau uaaggagauc     4440

ugucucccac ugaagauaga ucgguuggga caaauuaucg aaaucaguac cuucuga        4497


<210>  3412
<211>  4506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3412
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cgugauaaau       60

aaggcugaga acgaagguaa agauguccug ugcggcaucg aggcagccgg guccagaauu      120

auccccaugg acgcgacaau uaugggagau uucgaaaagg gcaauucugu uucacagacu      180

aaagacaggc gggagcaccg caguacgcga cguuuguacg aaagagguaa guuaaggcgg      240

gagcgccuga acagagugcu aagcguaaug ggguuucucc cugaucauua ugugucccaa      300

cuggacaggu acggaaaguu ccuuucuaau accgagccaa aacuggcuug gguccggaac      360

gccgauugua aguaucacuu uuuguucaug gacucguuuu cagaaaugcu ccaggaguuc      420

aaaacaaaua gcccgcagua caucagugaa gaguccaagg ugcccuauga uuggacucug      480

uacuaucugc gcaaaaaggc acucacccau aagguuacaa aagaggaauu agccuggaua      540

cugcuuaacu uuaaucagaa gcgaggcuac uuccaauuga gagacgaguc ugaagaggag      600

aaaaagccua gcaaaaucag gcaguacuuu gaacaccaga agguggucga uauuaucgac      660

accaacgagg uguauaaagg ccugaaggua cuaauuguua uccucgcuaa ugggauaucc      720

ggaaagauuu ucaaaaagga aaucccagau uggauuggug uggagaaaga caucaucguc      780

aagguggaca uugauaaaga cggcaaggau aaguuuaacg aagacggguc acugaguugc      840

cgguucaaua uacccacuga ggcagaaugg gauacagagu gggagcuuau caaacagaag      900

acccaaaacg accuggaugc cagcggaaaa acggugggcg aauacauuuu ugacacuuug      960

cuccguaauc cugagcagaa gauccgcggu aaacugguca gaaccauaga aaggcgguau     1020

uacaaggagg aaauuaacgc uaucuuaaag aaacagaagg aguuccaucc agagcugucu     1080

gaucgagccc ucuaugaaaa auguguugag gaacuuuacc ccaacaaucc ugcgcaccgc     1140

aacaugaugc ugauccacga cuuguccuau uuccuaguga augauauucu guuuuaccag     1200

agaccacuca agagcaaaaa gucacugauc gacgagugcc cguacgagag ucaucuuuau     1260

auugacaaga aaucugggga aaggaagccc guagcaguga aauguauagc caagucccac     1320

ccucuguacc aagaguuccg guuguggcag uuuaucagca accuccgcau uuaucagaaa     1380

acaaaggaag ucggaaauuc gcugaaagug gauguugacg ugacuaacga guucuuaaag     1440

ggcgaaaaug auuacgucga gcuguuugac uggcuaaaca ccaagaaaga gaucaagcaa     1500

gaagauuucc ucaaauauaa gccauuuggc uucacaaaag ggaauaucaa caauuacaga     1560

uggaacuaug uggaggacaa gauuuacccc ugcaauaaga cccauuacac gauacugaaa     1620

ggacuugaaa aggcugauau cacuuuugac gaguucauuu cucacgauuc cuuggccgaa     1680

gagaaccugu ggcacauccu cuauucagua aguagcaaag aggaacugaa gcaugcacuu     1740

gaguccuuug cugacaaaag gggucugucu auuaaguucg uugaugccuu uaucaauacc     1800

ccugcguucg aaaagagcua cggcgccuau ucagcaaaag cuauaaagaa auugcuccca     1860

cugaugcgua uggggaagua cuggaaagag gaaaacaucu gugacaguac acgggagcga     1920

auugauaaga ucauuacugg agaguaugac gaaaacauca gagacagggu gcgcgagaag     1980

uccauaaauu uaacagauau uacccuguuc cggggccuac cccucuggcu ggccugcuac     2040

gucguguaug acagacacag cgaagcuggu aacacggaga gguggaccuc uccgucagau     2100

aucgaccauu acauucgcaa auuuaagcag cacagucuuc gaaauccuau cgucgagcag     2160

auaguguugg aaacacugcg gacuguucgu gaugugugga ccgagguagg gggcaucgac     2220

gaaauucaug ucgagcucgg aagagaaaug aaaaacccca gcgauaagag gcgcaaaaug     2280

ucccugcaga ugcaagagaa ugagaacaca aauuuacgga ucaaggcccu gcuugcagaa     2340

uuuauuaacc cagaguuggg caucgaaaau gugagaccuu acucucccuc gcagcaggac     2400

cuccugaggc uauaugagga aggggccugg aacagcccag agauagauga gaaacagguu     2460

gacgaaaugc uggcuauucu caagaaguuu aaugagacug auuccucaaa acgacccacc     2520

aagagugaaa ucaaacgcua ccggcugugg cuugagcaaa aguauagauc uccuuacaca     2580

ggaaccccaa ucccguugag caaacuguuc acuacggcgu augaaauuga gcacgugauc     2640

cccagguccc guuucuucga cgauucauua acaaacaagg ucauuuguga gagcgaagug     2700

aauaagcuca aagguaacau gcugggcuac gaguuuauaa agucucacua uggggaaaaa     2760

guacagcuuu ccaguggaag cgaggugcag aucuucucug uuaaugacua cgagaaguuu     2820

gucaaagacc auuacuccaa gaacaaggca aaaaugaaga aacuguugau ggaugacauu     2880

ccugaaaagu ucaccgagcg ccagaugaac gauucacggu auaucaguag acucauugug     2940

agccugcuau cgaauaucgu gagggaaaaa ggcgagcaag aauccauauc uaagaacguc     3000

cugguuugca cugguggggu gaccgaccga cucaagaaug auuggggcgu gaacgacgua     3060

uggaauaaaa ucauucugcc acgguuuaag cgcaugaacg agaucacaga uagcaccgac     3120

uacacuucag ucaauacaag uggacacauu auccccgcca ugccucuuca uuugcagaaa     3180

ggcuucaaca agaaaagaau agaucacagg caccaugcua uggacgccau ugugaucgca     3240

ugugccgggc ggaauaucgu uaacuaucug aauaaccagu ccucuaagag cgaugugucc     3300

cgacacgacu uaagaaaucu ccugugcgag aaggacaaau cagaucgcaa ggaacuuauu     3360

auaaaaccau gggacacguu uacccaggau gucuacagcg ugcugaguuc uaucauuguu     3420

uccaucaagc aaaaccuaag ggugauuaau aaaacuaaga acaucuauca gcguaaagag     3480

gacggaaagg uaauaaucag caagcaggaa aacaauauua acuggugggc uauccggaaa     3540

ccccuccaua aggagacagu cucgggugaa gugaaucuga gaaaagagac cacaauuuca     3600

uugaaggagg ccaucgauaa cccggcaagg auaauugaca aagauuucaa gaagaaacug     3660

cgcgaauuaa uccucauggg cuacaaugag aagcugauca aaaaguauuu cuacgacaac     3720

aaugaaucuu ggcaagaugu gaaccuuaaa aagauuccug cguacuauuu uacuaaggac     3780

accaaugauc gauacuucgc uaguagaucc cuguuggacg agaacuuuga uaaaaagaaa     3840

aucgaaaaua aggucacgga caccgggaua cggacaauuc uccugaggca ccuagagguu     3900

gagggaggcg acagcaaagu ggccuucucu ccagaagguc uggagcgcau gaacaagaau     3960

aucgaaauuc uuaacggggg caagaaacau aagcccaucu auaaaguacg ugaguacgag     4020

aaggcagaaa aauuugccgu gggaaugaga ggcaauaagg cuagcaaauu cgucgaggcc     4080

gcaaagggga cuaaccucuu uuucgcugug uaugauaaug gaaagaaacg guccuuugcc     4140

accguuccuc ugaacgaagu gauaagguug cagaaggagg gccugcccuc agcgccagcc     4200

aacgaagagg guuuacuccc uaaauucguc cugaguccca augaccuugu guacguucca     4260

acagaggaug aacugcgcag cggguugcuc ccggagaacc cugcucccga acgaauuuau     4320

aagauggugu cuugcacugg auaccagugc uuuuucaucc acuucuccgu cucaggcgua     4380

cugugugaca aaaaggaguu uaguagccua aauaagaugg aaagagcaau cuccggugag     4440

augauuaaag agaucuguau uccacugaag gugaaccggc uuggcaaaau aauguacaau     4500

cucuga                                                                4506


<210>  3413
<211>  4506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3413
augaagacca uccugggccu cgaccuuggg acaaacagca uuggaugggc caaaauaucc       60

aucgaugaca augguaacua ccugagagau auucaguugg gcucuaggau cauucccaug      120

ucacaagacg uguuagggaa guucgauaau ggagucacug agagucagac ggcuguucgg      180

accggcuuuc gcggucugcg acguaucaga gaaaggagcc uacuccggcg cgagagacug      240

cacagggugc uucauguaau gggguuccug ccuccacacu augacuccgc aauaggaugg      300

aacaaagaag aaccgucuac auacggcaag uuuuugaauc auggcgaacc caaacucgcc      360

ugggagcggc agggggaugg aucgaugcgc uuccuguuua uggacucauu ccacaagaug      420

auggaggauu uugcgccuca ucagccagcu cugguggccg acggucgaaa gaucccccuc      480

gauuggacuu uauauuaccu gagaaagaag gcacuuaccc aaccuauuaa aaaggaagag      540

uuggccugga uccugcuaaa cuucaaucag aaaaggggcu auuaccagcu ccgaggggaa      600

gaggaggaag agaacccaac aaagcgugaa gaguaucacg aacugaaggu cgugagcguu      660

gaggcugcag aggaaggaaa aggcgguaau accugguaca acgugaaacu ugagaauggg      720

uggauuuacc accgccccag uaaaaucgac cuguccgauu gggucggaaa gacuaaacag      780

uuuauaguga caauggaaua ugaggccgac ggcauuaccu ugaagaaaaa caaggaaggc      840

gagguaaaga gaucuuucag gguuccugac gaggaugacu gggggcuccg gaaaaagcga      900

acggaaagcc aacuggaggc uuccggaaaa acugugggug ccuuuaucua cgaucauuua      960

cuggcggaac cagcagacaa gauucgcggc aauuucauca gaaccaucga gaggaaauau     1020

uacaagcagg aacucgccga uauucuucgg gagcagucaa aguaucacga ggaacugcag     1080

aguaaaaaga uauuggcuga cugcgccgag gaacuauacc gcagcaacca accgcauaga     1140

gauucucugc ucaaaaagga cauggucuau cugcuucuga cagauuugau cuuuuaccag     1200

aggccccuca aauccaagaa gucacugauu gcaaauuguc cuuacgagua uuacgaguau     1260

guggacaaag aaacugggga gaucaagcgu cagggaauaa aaugcguggc uaagagcaac     1320

ccauacuauc aagaguuccg guuauggcag uucguccuga accuacgacu cuacgauaga     1380

accgaggaca aagaaguuac agccgaguau cugcccagua aggaggacua cgcgaggcuu     1440

uuuguguacu ugaaugaucg caaggaaauu aaccaggaga cccugcucaa agacuucuuu     1500

aagcugaaaa agguaaaaca gcggucugaa aaggaguucc cugugagaug gaauuauauc     1560

gaagauaaag agaagaagua cccauguaac gagacgaggc acgaacuucu gauggccuug     1620

gaccgcgcag gcauggagcg auccuggcuc gaugacacug cucggcaaua ucgucugugg     1680

cacuuacugu acagcaucga agucaaagau gaggccgaaa gagcucuaag gaagcucaaa     1740

gacgaugacg aguuuguguc ggccuuccug aagguuaaac ccuuuaagaa ggauuauggu     1800

gcauacucug agaaagccgu gaagaaacuu uuggcuguca ugcgcauggg gucccauugg     1860

ugcgaagacg auauuuguaa ggagacaaaa gaacggaucg agcacauucu gcagggcgac     1920

guggaggaaa agauaaagga gagaaugaau cauaccgcgu auucacucgg agaacugagu     1980

gacuuccagg gcuuaccgga guggcuggca ugcuacguag uuuacgggag gcacagcgaa     2040

gccacugaua ucaaacauug gaagacaccu gagucccuua uggcuuauau uaacggauuu     2100

aaacagcacu cuuugcgaaa ucccaucgug gagcaaugua uccucgaaac ccugcgcacg     2160

guccacgaca uuuggaagga ggccggucau auagaugaaa uccacgugga gcuaggccgg     2220

agcaugaaau caaccaguga ccagagagca aggaugacag aaaacauucu gcguaaugag     2280

aacacuaauc uccgcaucaa gucccugcuu auggaguuga agaacgaugc cgacauuaaa     2340

gauguccggc cauacagccc uaugcagcag gaaauccuga gaauauauga ggaaggggcu     2400

uuacaagagc ucaccaagga agacaaugau uacgaggaga ucuucaaaau uucuaagauc     2460

ccccagccau cagccaaaga acugcuuaag uauaaacugu gguuggagca gaaguacagg     2520

agucccuaua caggaaagcc uauuagccuc uccaaacugu uuacuucugc auaccagauc     2580

gaacauauaa uuccacaauc gcgauacuuc gacgauagcu uuaacaauaa ggugaucugc     2640

gaguccgagg uuaacgcgcu aaaaucaaac augcugggcu augaguucau uaagaaucac     2700

gguggggaaa ucgugcacug uacccuccug ggcgacguaa aaguccagaa cgaaagugag     2760

uacaaggcuu ucgugacaga acauuaugcc aauaacaagu acaaaaagga gaaacuuuug     2820

auggaggaua uaccgcagga cuuucugaau cggcacuuaa acgacucucg cuauaucagc     2880

aagguuguga aaacccuccu guccaauauu gucagaacug aagaugagau ggaaacgaca     2940

ucaaaguucg ugguaagcug caccggaggc aucacugaca ggcuuaagaa agauuggggg     3000

cugaacgacg uguggaacuc uauuguuuac ucccgguuug agcgacuaaa ugaacucacc     3060

aagagugaug cauucggaag augggagaau aaagagggua agcgcgucuu ucaaacaagc     3120

gugccccugg aauugcagag gggcuucaac aaaaagcgua ucgaccaucg gcaccaugcc     3180

auggaugcuc uggugauagc cugugcaucu agaaauauua ucaacuauuu aaauaacgag     3240

uccgcuaacu caccuaagaa aagggaagac cuccgccagc agcugugcga uaagaaucga     3300

aucauuagaa aaccauggga gaguuuuacc caagacgccc gggcggcccu ugaagauguc     3360

guugugagcu ucaagaacua cgugagggua aucaauaaaa cuacaaacua cuaugagcgc     3420

uacggggcug acggagagaa gacgauugca aagcagaaaa auucggaaau gugggccguc     3480

cguaagccca ugcacgaggc uaaauauuac ggcagaguga accugcagcg gaagaccgaa     3540

guuagguuga aagccgcacu cgaggacaua ccuuccaucu gcaauaagga gcugcgcgau     3600

uauauuaacc ggcuagugaa aucuggucug aaugcgagac agcuucucgc ccacuuuaag     3660

agcacugacu acuguuggaa caagcaaaau gucgauaaag uggaaguuug gcgguucuca     3720

gacgagaagg aaccacuggu ggcuaucagg gagaguuugg auacauccuu ugacgccaaa     3780

cgcauugaua ccaucacaga cacugggaua cagaagaucc uguuaaacua ucucgaagca     3840

aaaggcggag aucccgcuau ugccuucacc ccggagggca ucgcagagau gaaucagaac     3900

auggcccugu acaauaacgg gaagaugcau cagccuauuc uuagaguaag gcugucuaag     3960

aaacggggaa agaaauacca agucggccag acggguugca aggcuaaaca guuugugauc     4020

agcgaauccg ggaccaacuu guauuucgcg auauacgagg acgaagaucg uaaucgcaca     4080

uauaacacua uuccacucaa ugagguggcc gaacgacuga agcaaggacu aucacccguc     4140

ccugagaaga acgagaaaga aguuccccug aaguucuacc uuagcccaaa ugaccucgug     4200

uauguaccua ccgaggaaga gauccugaug aaagaaugua aguugaacaa aaauagaauc     4260

uacaaguuua uugauaguuc uggcacaacu gcaaacuucg ugccccacag guccgcuagc     4320

uuaauauaua augucgacaa gaaacuggcc gaggccuuuu gcaacgacga gaucauucag     4380

aaugaauacg gugugggcuc gccacaguca aagaaccaga aagcucucac cggggagaug     4440

aucaaggcag uuuguuggaa acuggaagug gaucggcuug gaaauauuau ccgcgucaua     4500

aaguga                                                                4506


<210>  3414
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3414
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

gguaauauaa acgaugacgg cuccgagcag cuugugaaaa uccaggcuuc ugggucaaga      120

auuaucccca uggaugcagc caugauugga gacuucaaua agggcaacag uaucagccaa      180

acagcggaaa ggacucggcu gcgcgguaua cgacguuugu ccgagagaua cuuacugagg      240

cgggaacgcc uacacagaau ccucgauauu cugggguuuc uuccuucuca uuucgcuagg      300

gaccuggauc gguauggaaa aaucguaaag gacaaagagc caaaguuggc cuggcgcaag      360

aaugaagcag gccaguuuga guucauuuuu cagaacucgu ucaaagagau gcucgaagau      420

uuuaagcuga aucacccgaa ccugaucacg gacgauaaaa aggugcccua cgacuggacc      480

auauauuacc uccgaaagaa gggcuuaaca ucaaagauua gcaaagagga acuggccugg      540

auccuuuuga auuucaacca aaagagaggg uauuaccagc ugaggggaga ggaggaagag      600

gaaaauaaaa acaagcaggu cgaguuuuau gcucuaaaag ugauugcagu ugaagauagu      660

ggugagaaga aaggcaagga cacuugguac aaugugcauc ucgagaacgg guggaucuac      720

cggcguaccu ccaacaugcc ucuggacugg gucggaaaga caaaagaguu caucgugacc      780

acugaucuug aaaaggacgg cacacccaaa aaggauaaag aagguaauau uaagcgcucu      840

uuuagagccc caaaggagga cgauuggagc cugguuaaaa agaaaaccga agcugacaua      900

gaucagacgc acaagacugu agggaccuau aucuacgaca cauugcucgg aaacccuucc      960

cagaaaauua ggggccggcu ggugcgaacu aucgagcgca aguauuacaa ggaggaauua     1020

aaacugauac ucgauaagca aaaagaguuc cauccagaac uucaggacag agcccuguau     1080

gcggcaugcg ucgaggaauu guacaccauu aaugaggccc acaggaacaa uaucggcaac     1140

cgggauuuua cauaucuauu ccuggaggac auccucuucu accagcgccc ccugaagagu     1200

aaaaagucac uuauugacaa uuguccuuac gaagagaacc acugccugga uaaggaaacc     1260

ggggagauca aauuuuuccc agugaagugu auugcuaaaa gccauccguu guuucaagag     1320

uucagacucu ggcaguuuau agugaaucug aggaucuauc guaagggauu agcccaggac     1380

gucgauguua cgaacgaacu gcuaaaaacu gaggaagacu acguggcacu cuucgauugg     1440

cugaauggua agaaagagau ugaccaaaag gcuuuucuua aguauccccc uuucggcuug     1500

aaaaaggaag uagagaacua ccgguggaau uaugugcagg auaaaucuua cccaugcaac     1560

gagacacgau cccugaugcu cgccagacug gaaaagugug acaucucaau cgaguuucuu     1620

accaaagaaa augaggaagc gcuguggcac auuuuguaua gcgucgagga uaagaacgag     1680

auagugaagg cccuccagaa auucgcagac aagcauaacc ugaaugcuag uuuuguugau     1740

guguuccaga aauuucccuc uuucaagaaa gaauacgggu ccuacagcgc caaggcuauc     1800

aagaaauuac ugccucuaau gaggauggga aaguauuggu cggaggacuc uauugauaaa     1860

ggcacucgcg aacggaugga caagaucauu acaggugagu acgacgagaa aaucgaaaac     1920

agagucaggg agaaggccau acaccucgca gauaucuccg aguucaaggg gcugccacuu     1980

ugguuggugu gcuauauugu auacggccgc cacucagaaa guaaagaaau cgccaagugg     2040

gagagccccg cugacauuga uuccuaucug aaaucuuuua agcaacauag ccuccgaaau     2100

ccgaucguug agcagauaau uaucgaaacc cugcgggugg uccgugacau uuggaaacag     2160

gugggaaaca ucgaugagau acacgucgaa uuaggcagag agaugaagaa uccugcggac     2220

aaaaggcgca agaugacgca gcaaaucuca gaaaacgaga auaccaaccu gcggauuaag     2280

uaccuuuuga cagaguuccu caaucccgaa uaugaggugg aaaacguuag accauacagu     2340

ccuucccagc aggauauccu gaggauuuac gaggaagggg ugcuaaauag cguaucuaac     2400

cugcccgagg agaucucaga cauacucaaa aaguuuacug aaaccgaucu gaaaaagcga     2460

ccaagucgca gcgagguccu ucgguauaaa uuguggcugg aacagaagua cagauccccc     2520

uauacaggag aggugauucc uuuagguaaa cucuucacuc cagcauacga aaucgagcau     2580

guuaucccgc aaucuaggua uuuugacgau ucguucagca auaaagugau uugugaggcc     2640

gaagucaaca agcugaaauc caaucuucug ggccacgagu uuaucgugaa gcaucacggg     2700

gaaauuguag aguugcccuu cggcaaaaag gugaagauau uuaccguuga cgaguacgaa     2760

caguucguca aagauaacua uucacguaca cgcggaaaga ugaaaaagcu ccugguggac     2820

gacaucccug aggaauuugu ggcucggcag cuaaacgaua guagauacau uucuaaaguc     2880

guuaagagcc ugcucuccaa uaucgugagg gagaagggug aagaggaggc cauuagcaaa     2940

aacgugaucc caugcaccgg gggcguaacu gaccgacuga agaaagauug gggagucaau     3000

gacgugugga acaagauaau ccuuccccgg uuccagcgcu ugaaugaacu gacgggcaca     3060

aacaaauuua ccacuauguc auuaaauaac cacgagauuc cugcaaugcc acucgaacug     3120

caaaaggggu ucaauaagaa aagaaucgau cauaggcacc augccaugga cgcuauuauc     3180

auagccugug caaaccggaa uauuaucaac uaccuuaaua acgagucugc gaccgcuaag     3240

gccgaacugu cccgauauga ucuacagaga cuccugugcg acaaagcaaa gacagaugac     3300

aauggaaacu acaaaugggu uauccgcaag cccugggcca guuucaccca ggaugugcac     3360

ggugucuugg aggacauuau agugagcuuu aaacaaaacc ugaggguuau caauaaggcu     3420

acuaaccacu ucuuacauua uaaugaggaa ggcaagaaaa uuuuugugaa gcaggagaaa     3480

ggggacucuu gggccauccg uaaguccaug cacaaagaua caguauucgg agaagucaac     3540

cuccggagaa uuaagacggu ggcacugaau gaggcuauga agaacccgca gucaaucgug     3600

gucaaagacu uuaagaggaa acuucuggaa uugcucaauc ugggcuucga ugccaagcgc     3660

aucaaaaagu acuuugagga caaccgagag accuggagug auauaaaucu aagcaagauu     3720

acuguuuauu acuucucgaa agacacaaag gauagauuuu ucgcgacccg gaaaccucug     3780

gacacauccu uugauaggaa gaaaaucgaa aacaauauua cugacaccgg uauccagaag     3840

auacuucucc gccaucugga guugaaggau aacaauccag acauugccuu cucucccgau     3900

gggaucgacg aaaugaaccg uaauaucauu caacugaaca auggcaaaua ucaccagccu     3960

aucauaaagg ugagauggua cgagcaggcu gacaaauucg caguaggaca gacgggcaac     4020

aagagcucaa aauuugugga ggccgcuaag gggaccaacu uauucuuugc cgucuacgaa     4080

aguaauauuc ucgaugagaa aacaaacacu aucauuaaga agcggaauua ugcaaccauc     4140

ccccugaacg uggcgauaga aaggcaaaaa cagggacuuu ccguugcccc agaggaagag     4200

aauggcaacg acccuauuuu cgugcugucu cccaaugauu uggucuaccu cccaacaacu     4260

gacgagcugg cuaacggugu gguugcccag ccgcuagaua aggaacgcau cuauaaaaug     4320

gugagcugua ccgggaauga gggacauuuu aucccugcac gaauugcuaa ccccauccug     4380

cagacgauug aacuuggcuc caauaacaag gcccaaaaag cauggacaga cgagaugaua     4440

aaggaaaucu gcaugccaau uaaggucgau agacucggua auauccugga gucaagcagu     4500

uugcacaaaa aguga                                                      4515


<210>  3415
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3415
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

gguuccguga augaugacgg cucugagcag cuuguaaaaa uacaggcuuc agggaguaga      120

aucauuccca uggaugcagc caugaucgga gacuucaaca agggcaauag cauuucccaa      180

acagcggaaa ggacucggcu gcgcggugug cgacguuugu cugagagaua cuuacugagg      240

cgggaacgcc uacacagaau ccucgauaua cugggguuuc uuccuuucca uuuugcucag      300

gaccuggaua ggcacggaaa aaucgucaag ggcaaagagc caaaguuggc cuggcggaag      360

aacgaagcag gccaguucga guuuauuuuc caagacucgu uuaaagagau gcucgaagau      420

uucaagcuga auuauccgaa ccugaucacg gacgauaaaa aggugcccua cgacuggacc      480

auuuauuacc uccgcaaaaa gggguuaaca ucaaagauca gcaaagagga acuggccugg      540

auacuuuuga auuuuaacca gaagcgagga uauuaccagc ugagagguga ggaggaagag      600

gaaaauaaag auaagcuagu ugaguucuau gcucucaaag uggucgcagu ggaagacagu      660

ggcgagaaga aagggaagga cauuugguac aacguacauc uggagaaugg auggguuuac      720

aggcggacuu ccaacauguc ucuugauugg gugggcaaga ccaaagaauu uaucgucaca      780

accgaccugg agaaggaugg uacuccuaaa aaggacaaag aagggaaugu gaagcguagc      840

uuccgcgccc caaaggagga ugacuggucc uuggugaaaa agaaaacaga agcugauauu      900

gaccagaccc acaagacggu cggagccuau aucuacgaua cucuccuggg caaccccuca      960

caaaagauca gaggcagguu aguucggacc auugagcgaa aguauuacaa ggaggaacug     1020

aaacucauac uugagaagca gaaagaauuu caucuggagu ugcaggaccg cgcgcuauau     1080

gcagccugca ucgaggaacu guacccuauu aacgaggcuc acagaaauaa cguggggaau     1140

agggauuuca cauaucucuu ccuggaagac auccuuuucu accagcggcc acugaagagu     1200

aaaaagagcu ugauagacaa cugucccuac gaggaaaauc aaggacucga uaaggagacu     1260

ggugagauua aaucuuuccc uguaaagugc aucgccaaau cccacccacu guuucaggaa     1320

uuucgcuuau ggcaguuuau cauuaaccug agaaucuaua ggaaggaccu agcacaagau     1380

guggacguca ccaaugagcu ccugaaaaca gaagaggauu acguggcucu uuucgacugg     1440

uugaacggca agaaagaaau ugaucagaag gccuuucuga aguauccggu uuucgggcuc     1500

aaaaaggaga uagagaauua ccguuggaac uaugugcagg acaaacccua cccuuguaau     1560

gaaacccggu cacugaugcu ugcgcgacug gagaagugcg auaucagcau ugaauuuuug     1620

acgaaagaga acgaagaggc ccucuggcau auccuguaua gugucgagga caagaaugaa     1680

aucgugaagg cauuacagaa auucgcugau aagcacgacc ugaacgccuc uuuuguagau     1740

guuuuccaaa aauuuccacc cuucaagaaa gaguacggau ccuacagcgc uaaggccauu     1800

aagaaacuac ucccucugau gagaaugggc aaguauuggu cggaaaauuc uauagacaaa     1860

gguacuaggg agcgcaucga caagauuauc acaggggaau acgaugagaa aauugagaac     1920

cgggugagag aaaaggcaau ccaucuugcc gacgucuccg auuucaaggg cuugccacug     1980

uggcucgugu guuauauagu cuacggaagg cacucagagg cuaaagaaau cauucagugg     2040

aagagucccg cggacaucga uagcuaucug cgcuccuuua aacagcacuc uuuacgaaac     2100

ccgauugugg agcagaucau aauugagacc cugcggguug ugcgugacau cuggaagcaa     2160

guaggcaaua uugaugaaau ccaugucgag cuugggagag aaaugaaaaa cccugcagac     2220

aagaggaaaa agaugacuca gcagaugagc gagaaugaaa acacaaauuu gcgcauaaag     2280

uaccuccuga ccgaguuccu aaaccccgag uaugaagugg agaauguucg gccauacuca     2340

ccuagucagc aagauauccu gagaauuuac gaagagggag ugcucaacuc cgucagcgac     2400

cugcccgaag agaucucuga uauucuuaaa aaguuuacgg agaccgacuu gaaaaagagg     2460

ccaucacgaa gugaagugcu gcgcuauaaa uuauggcucg agcagaagua ccggagcccc     2520

uauacaggug aaguaauccc ucugggcaaa cuuuucacuc cagccuacga gauagaacac     2580

gugauuccgc aguccugcua uuuugaugac ucuuucucga auaaaguuau cugugaggcu     2640

gaggucaaca agcugaaaag caauuugcuc gggcaugaau uuaucgugaa gcaccaugga     2700

gagauugugg aacugcccuu cggcaaaaag gucagaaucu uuaccguuga cgaguacgag     2760

caguucguga aggauaacua uuccaggaca cguucaaaaa ugaagaaacu acugauggac     2820

gauauuccug aagaguuugu gacucgccaa cucaaugaca gucgguacau aucuaaggua     2880

gucaaaagcc ugcuuuccaa caucgugaga gaaaagggug aggaagaggc cauuucaaag     2940

aauguuaucc caugcaccgg gggcgugaca gauagguuga aaaaggacug gggaauuaac     3000

gaggucugga acaaaaucau acugccccga uuccagcggu uaaaugaacu caccggcacu     3060

aacaaguuua cgaccaaaaa uguggggauc caggagauuc cuacaaugcc acuggaacuu     3120

caaaagggau ucaacaagaa acgcaucgau cacagacacc augcaaugga cgccauuauc     3180

auagcuugug ccaauaggaa cauuaucaau uaccugaaca augagagcgc aacugcgaag     3240

gcugaacuau cucgguauga ucuccagaaa augcugugcg acaaggccaa aaccgauaac     3300

aaugguaacu acaagugggu uauccgaaaa cccugggcau ccuucacaca ggacaccuau     3360

uuggcccugg agaauauuau agugaguuuu aagcagaacu uaagaguaau caauaaggcu     3420

acuaaccgcu uccuccacua caacgaggaa ggcaaaaaga uuuuugucaa acaagggaag     3480

ggagacaauu gggccaucag gaaaagcaug cauaaggaua caguguucgg cgaggugaac     3540

cugcgucgga uuaagacggu cgcacuuaau gaagcuauga aaaacccgca gucuaucguu     3600

gugaaggacu uuaaaagaaa gcuguuggag cucuggaauc uggguuucga ugccaaaagg     3660

auaaagaagu auuuugaaga caaccgcgag accugguccg auaucaaucu aucaaaaauu     3720

gagguauacu auuucaguaa ggacacuaaa gaucgauuuu ucgcgacaag aaagccucug     3780

gacaccagcu uugaucggaa aaagaucgaa aacaauauua cagacacugg gauccagaag     3840

auacuucuca ggcaccugga guugaaagau aacaauccag acauugccuu cucgcccgau     3900

ggcaucgacg aaaugaaccg caauaucauu cagcugaaca auggaaagua ccaccaaccu     3960

aucauaaaaa uucguuggua cgagcaggcu gacaaguucg cagugggcca gaccgggaac     4020

aaauccucua aguuugucga ggccgcuaaa ggaacgaauu uauucuuugc cguguaugaa     4080

agcaacaucc ucgauaagaa gaccaacaca auuaucaaaa agagaaauua cgcaacuaua     4140

ccccugaacg uugcgauuga gcggcagaaa caaggccuuc caguggcccc ugaagacgag     4200

aaugguaacg aucccaucuu cguccuguca ccaaaugacu ugguguaucu cccgaccgau     4260

gacgaacugg cuaacgggau cauugcccag ccucuagaua ggggacgcau cuacaagaug     4320

guuaguuccu cuggcgagca gugcuuuuuc auuaaacaua uaguggcaaa uguccuggua     4380

gacaaguuug aguucagccc ccuuaacaag auggaacgag cucucacagg ugagaugauc     4440

aaaaugauuu guaucccaau caagguggau agacugggca auguuuugga auccucaagc     4500

aguuauaaaa aguga                                                      4515


<210>  3416
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3416
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

gguuccguga augaugacgg cucugagcag cuuguaaaaa uacaggcuuc agggaguaga      120

aucauuccca uggaugcagc caugaucgga gacuucaaca agggcaauag cauuucccaa      180

acagcggaaa ggacucggcu gcgcggugug cgacguuugu cugagagaua cuuacugagg      240

cgggaacgcc uacacagaau ccucgauaua cugggguuuc uuccuuucca uuuugcucag      300

gaccuggaua ggcacggaaa aaucgucaag ggcaaagagc caaaguuggc cuggcggaag      360

aacgaagcag gccaguucga guuuauuuuc caagacucgu uuaaagagau gcucgaagau      420

uucaagcuga aucauccgaa ccugaucacg gacgauaaaa aggugcccua ugacuggacc      480

auuuacuauc uccgcaaaaa gggguuaaca ucaaagauca gcaaagagga acuggccugg      540

auacuuuuga auuuuaacca gaagcgagga uacuaucagc ugagagguga ggaggaagag      600

gaaaauaaaa acaagcuagu ugaguucuac gcucucaaag uggucgcagu ggaagauagu      660

ggcgagaaga aagggaagga cauuugguau aauguacacc uggagaacgg auggguuuac      720

aggcggacuu ccaauaugcc ucuugacugg gugggcaaga ccaaagaauu uaucgucaca      780

accgaucugg agaaggacgg uacuccaaag aaggauaaag aagggaacgu gaagcguucu      840

uuccgcgccc ccaaggagga cgauuggagc uuggugaaaa agaaaacaga agcugacauu      900

gaucagaccc auaagacggu cggagccuac aucuaugaca cucuccuggg caacccuucc      960

cagaaaauca gaggcagguu aguucggacc auugagcgaa aguacuauaa ggaggaacug     1020

aaacaaauac ucgagaagca gaaagaauuu caccuugagc ugcaggaucg cgcguuguac     1080

gcagccugca ucgaggaacu auauccaauu aaugaggcuc acagaaacaa uguggggaac     1140

agggacuuca cauaccuguu ccucgaagau auccuguuuu aucaacggcc ccuuaagagc     1200

aaaaagaguc ugauagacaa uuguccuuac gaggaaaacc agggauugga caaggagacu     1260

ggugagauua aaucauuccc aguaaagugc aucgccaaau cucauccgcu cuuucaggag     1320

uuccgccugu ggcaauuuau cauuaauuua agaaucuaca ggaaggaucu ggcacaggac     1380

guggauguca ccaacgaacu acucaaaaca gaagaggacu auguggcucu guucgauugg     1440

cuuaauggca agaaagaaau ugaccagaag gccuuuuuga aguaccccgu uuuccgucug     1500

aaaaaggaga uagagaacua ucgguggaau uacgugcagg auaaaccuua uccauguaac     1560

gaaacccgau cccucaugcu ggcgagacuu gagaagugcg acaucucaau ugaauuucug     1620

acgaaagaga augaagaggc cuuguggcac auccucuaca gcgucgagga uaagaacgaa     1680

aucgugaagg cacugcaaaa auucgcugac aagcaugauu uaaaugccag uuuuguagac     1740

guuuuccaga aauuuccccc uuucaagaaa gaguaugggu cuuacuccgc uaaggccauu     1800

aagaaacugc uaccacucau gaggauggga aaguacugga gcgaaaacuc gauagauaaa     1860

ggcacucgcg cacggaucga caagauuauc acaggugagu augacgagaa aauugaaaac     1920

agagugaggg agaaggccau ccaccuggcu gaugucucug acuucaaggg gcuucccuug     1980

uggcuggugu guuacauagu cuauggccgc cacuccgaag cgaaagagau cauucagugg     2040

aagucaccgg cagauaucga caguuaccuc cggagcuuua aacagcauuc ccugcgaaau     2100

ccuauugugg aacaaaucau aauugagacc uuacguguug ugagagauau cuggaagcag     2160

guaggaaaca uugacgagau ccacgucgaa cugggcaggg agaugaaaaa ucccgccgau     2220

aagcgcaaaa agaugacuca gcagaugucu gaaaacgaga auacaaaccu ucggauaaag     2280

uauuugcuca ccgaguuccu gaauccagaa uacgaggugg aaaacguuag accuuauagc     2340

cccucacaac aggacauccu aaggauuuac gaggaagggg ugcugaauag ugucuccgau     2400

cucccagagg aaaucagcga cauucugaaa aaguuuacgg agaccgaucu uaaaaagcga     2460

cccucucgcu cagagaucuu gcgguacaaa cugugguuag aacagaagua uagaaguccu     2520

uacacaggag aggugauacc acucgguaag cuguucacuc cggcuuauga aauugagcau     2580

guaauccccc agagcugcua cuuugacgau uccuucucua acaaagugau cugugaagcc     2640

gagguuaaua aacuuaaauc gaaccuguug ggccacgagu uuauugucaa gcaucacggg     2700

gaaaucgugg agcucccuuu cggcaaaaag gugaggauuu uuaccgucga cgaauaugag     2760

caauucguua aggacaauua cagccguaca cgcuccaaaa ugaagaaacu gcuaauggau     2820

gacauaccag aggaauuugu gacucggcag cugaacgauu caagauauau caguaaggug     2880

guaaaaucuc uccugagcaa uauugucagg gagaagggag aagaggaagc aaucuccaag     2940

aacgugauuc ccugcaccgg ugggguuaca gaccgacuua aaaaggauug gggcaucaac     3000

gaggugugga auaaaauaau cuugccucgg uuccagcgcc ugaacgaguu aaccggaacu     3060

aauaaguuua cgaccaaaaa cgucggccuc caagaaauuc caacaaugcc ccuggagcuu     3120

cagaaggggu ucaauaagaa aagaaucgac cacaggcauc acgccaugga ugcuauuauc     3180

auagccugug caaaccggaa uauuaucaac uaccugaaua acgaaucagc gacugcuaag     3240

gccgagcuaa gccgauacga ccuccagaaa augcugugcg auaaggcaaa aaccgacaau     3300

aacggaaauu auaagugggu gaucagaaaa ccgugggccu cuuucacaca ggauaccuac     3360

uuggcucugg aaaacauuau aguuuccuuu aagcaaaauu uacgcgugau caacaaggcc     3420

acuaacaggu uccuccauua uaaugaggag gguaaaaaga uuuuuguaaa acagggcaag     3480

ggggacaacu gggcaauccg uaaaaguaug cacaaggaca cagucuucgg agaagugaau     3540

cugcggagaa uuaagacggu ggcucuuaac gaggccauga aaaauccuca gagcaucguc     3600

guuaaggauu uuaaaaggaa gcuguuggaa cucuggaacc ugggcuucga cgcgaaacgc     3660

auaaagaagu acuuugagga uaaucgagaa accuggucug acaucaaccu auccaaaauu     3720

gagguguauu acuucucaaa ggauacuaaa gacagauuuu ucgccacacg gaagccacug     3780

gauaccaguu uugacaggaa aaagaucgag aauaacauua cagauacugg uauccagaag     3840

auacuucucc gccaccugga auugaaagac aauaaccccg auauugcuuu cagcccugac     3900

gggaucgaug agaugaaucg uaacaucauu caacugaaua acggcaagua ucaucagccc     3960

aucauaaaag uaagauggua cgaacaggca gacaaguucg ccgugggaca gaccggcaau     4020

aaauuuucga aguucgucga ggcugccaaa gggacgaacu uauuuuucgc aguguacgag     4080

uccaacauuc ucgacaagaa gaccaauaca aucauuaaaa agcggaacua ugcgacuauc     4140

ccacugaaug uugccauaga aaggcaaaaa cagggacuuc cuguggcucc cgaggaugaa     4200

aacggcaaug acccaauuuu uguccugucu ccgaacgauu ugguguaccu cccuaccgac     4260

gaugagcugg ccaaugguau caucgcacag ccccuagacc gcgggcgaau uuauaagaug     4320

guuagcucaa guggagaaca auguuucuuu aucaaacaca uuguggcuaa cguccuggua     4380

gauaaguucg aguuuucccc acuuaauaag auggagagag cccucacagg cgaaaugaua     4440

aaaaugaucu gcauucccau caagguggac cggcugggua acguuuugga gucuagcucc     4500

ucacauaaaa aguga                                                      4515


<210>  3417
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3417
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugauagag       60

aaugauuuca acaggaaaga agguaagauc gaggcuuugg gcucccggau uauccccaug      120

ucucaggaca uuuuagggaa auuugauuca ggagugacag ucagucaaac ugcagcccgc      180

acgaauuacc gaggcauccg uagacuguau cagagggaca accuacggcg cgaaagacuc      240

cacaggguuc ugaauauacu ugguuuccug ccuaagcauu acgcggagag caucgauuuu      300

gaagaccggu uggggcaguu caaaccagag accgagguga agcucccgua ucgcaaggau      360

uccgacgaua acuaccacuu uauuuuccag gaaucuuuua augagauggu agaagaguuc      420

caauuuaaaa accccucgcu guucuauaca aaggcuaaug gagagcaguc aaaaaucccu      480

uacgacugga cucuguauua ccuccgaaag aaagccuuaa gcaagaagau uaguaaagag      540

gaacuggcau ggauccuuuu gaacuuuaau cagaagagag gcuacuauca gcugaggggc      600

gaagaggaag ccgauuccaa agacaagcua gaggaguacc augcucucaa agugucugau      660

guccaagcaa ccgaagacac aaacgccaag gggaccuggu auaaugugau acuggagaac      720

ggauggauuu accggcguca gagcaaagac ucccuuuucg auuggaaggg uaagacucgc      780

gaauuuaucg uuacaaccuc acuggagaaa ggcggggcug ugaagauuga caaagaagga      840

aaugagaaga gaaguuucag ggccgucgau agcgagaaag acuggaucgc gaucaagaag      900

aaaacggaaa acgauuuggc aaacgccggc acuaccgugg guguauauau uuacgagaca      960

cuccugcgga auccagacca gaagauacga gggaaauuaa ucaagacuau ugaacgcaaa     1020

uauuaccugg aggaacucaa ggcuauccuu gccaagcaga uacaagagca cgcagagcug     1080

cagaacagau cuuuguacaa agauugcauu gaggaacuau aucagggaaa ugaagcucau     1140

aagaacaaua ucaaagagaa cggcuucgac uaccuguuuc ucgaggauau cauuuucuau     1200

caaaggcccc ugaaguccaa aaagucaacc aucagcaauu guccuuacga aagucggguu     1260

uuuauuaagg acggcaaaaa ggagauacag ccaaucaaau gcauuucuaa guccaacccc     1320

aucuaucagg aguuccgccu uuggcaguuu auccacaauc ugaaaauuua cgaaaaggaa     1380

gccauagaug acgggaaaac agugaucaac aaggacguca ccacggaguu cuugaauacu     1440

caagaggcgu augccgcacu cuuugaguuc cugaacgaua aggcugaaau uagccagaau     1500

gccuuacuga aauacuuuaa gcuaucgaca aacacccaca gauggaauaa cguggaagac     1560

aaaaaguacc cuuguaacga gacuaggucu gaauuuauca aacgucucuc caagauggau     1620

ggaauugacc uggguaagac acuuucacca gcuuuugaga aaaguuugug gcauaucaua     1680

uauagcguga aggauaaaaa gcaguacguc cgggcccugg agaccuucgc aaugaaaaau     1740

ggccucgaca ucaaccaguu ucgagaaaau uucgagaagu ucaagccgua ugauuccgcc     1800

uacggggcuu auucugaaaa agcgcugaag aaaguucuuc cccugauuag aaggggaacg     1860

uacuggaaga aagacgcaau cccugccgcu gugcaagagc gcauuguaaa caucaugaag     1920

cggauagaaa gcguggauuu uaauuuggag aagauugaga acuucgccga cgaugacauc     1980

ccaaaacagc uccugaaguc auuugcaaaa ggcaaggauc ccaugcaggg uuuaaauacc     2040

uaucaggccu gcuacgcugu cuacaacaga cacagugaag uguccgacau ucaacugugg     2100

aaaagcccug aggacaucac aaaguaucua gauucuuuca aacagcauuc acucaggaau     2160

ccaauaguug aacagguggu cauggagacu cugcgcgugg uacgagacau cuggcaguac     2220

uaugggaacg gcgccaagga uuucuucagu gagauucacg uugaacuugg acgggagaug     2280

aagaaugaca aaaagacccg ugaaagaauc agcaaaucca uuacagagaa cgaaaauacu     2340

aacgagagga ucaagaauau auugaaagag cugaugggcg augaagcacu ccaaggggac     2400

auucgccccu acucuaaggg acaccaggau auccugaagu uauaugagga agguguguuu     2460

gcgaacgcuc cggagaccua caaaaacauc aagauugacg auaucgaaaa gauucggaag     2520

aauaacucgc cuagcaaauc cgacauaguc aaguauaagc uguggcuuga gcagggcuac     2580

aucucacccu acacagggag uccaauuccu uugucuagac ucuucaccac ugauuaucaa     2640

aucgagcaua uuauccccag gagccgauac uuugacgauu ccaugggaaa uaaaauaauc     2700

ugugaaucag ccgugaacga ggaaaagacg aauaaaacag cauaugaguu cauuaaggaa     2760

aacgccggcc gcaucauuga ccugggucag gggaaacagg ucccacuauu ugaccuggag     2820

gcuuacgaga gccacugccg gggcuauuuc aagggaaaua ccaagaaacu caaguuucug     2880

cuuucugaag auauccccga gggcuucaua aacagacagu ugaaugacuc cagguacauu     2940

aguaaagugg uuacuagccu guuaucuaac gugguacgug aagaggggga gcaagaagcc     3000

accuccaaga aucucguccc ucugguggga acaaucaccu caaaacuuaa gaaggauugg     3060

ggucuggagg caaucuggga cgauuugauu gcuccacgcu ucgaacggau gaaccagcuc     3120

acuaaaagua cagacuuuag aaaggagguu auagauggcc augggaauag guauuucauc     3180

aacacggugc cggacgauau uaaaaaggga uuuagcaaaa agcgaaucga ccaccggcau     3240

cacgcccugg augcgcuagu cauugccugu accacuaaag accacgugaa uuacaucaca     3300

ucgcugaaca cccagcgcaa caauagaucc cucguaucua agcugaggaa gauagaagag     3360

aaaacaauca aggacaaacg gacuggccag cccaagacca uuaaaaucgc uaaggcauac     3420

cauaagccuu ggccaaacuu cacgaaagag accuuugaaa aacuuagcaa aacagugguu     3480

ucauucaagc aaaaucagcg aauuaucaac aaaacuaaua acaagaccug ggguugggag     3540

aaggucgaug gggugaugaa aaagguguug aaaaagcagg agaaaagaaa uugggccaua     3600

cgcaagccca uucacaagga aacauauuac ggcaaaguca agggauuuga caguccuaaa     3660

ggcaagauca cuaccgcuac gaggaaaucc cugcgugaaa ucacaaacga gcggcaguua     3720

aauaccauua cugauucugg gaucaagaaa auacucaaca aucaucugaa gacauucauu     3780

gacgagagcg gaaagaucaa cuuugaacca gccuucaauc aagauggcau ugaccuucug     3840

aacaaaaaua uccagcagcu aaacgagggu aagaaucacc aacccauaua uaaaguuaga     3900

auguacaccc acaacacuau guuuuccgug ggggaugaag gaaacaaguc aaccaaauau     3960

gugauuccgg agagcggcac aaaucucuuc uucaacguau acugggacga agagaauaac     4020

aagaggaauu uugagacggu cccucugaac cgcgugaucg cauuccagaa gcagaccagu     4080

cauaucccac gaaaagauag aacugacguu cccauugaua auacauuggg ucgguuucug     4140

uucaccuuau cuccugacga ucucguguau cugcccacag acgaagaguc cgagagcuuu     4200

acuaccuucg aaaucgacuc gcuuaacuca aagcaggucg cgaauauuua caaauuugug     4260

gauggcucug ggacgacagg aaacuucguu ccagccgagg cugccagggu gauagcaaau     4320

aucaaggaca aaaagguacg caguaacuuu ugcaagcgua auaaccuguc cgauaaacaa     4380

uugauuaaga augaguucgg ccucgggagc ccucagcuga aaaaccagaa cucucuagac     4440

ggaagacaga ucaagagcau cuguuggaaa cuggaaauua aucggcuugg caagauaauc     4500

aaggucauua aauga                                                      4515


<210>  3418
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3418
augaagaaag ugcugggccu cgacaucggg accaacagcg ucggaugggc cguugugaau       60

cagaacgaua agggugaguu ccuuuccauu gaaaagauag gcucuagaau cauucccaug      120

ucacaagacg uaauggauaa guuuggggcu ggacagacag agaguagcac ugcacagagg      180

acgcaguacc ggggcgugcg ccgacugcgu gaaagauccu uguuaaggcg ggagcgccug      240

cacagagucc uacauauccu ccacuuccug ccucaacauu augacgccgc gauugguugg      300

aauaaaaagg aaaacaagac cuacgggaaa uuucuuccag gaguggaggu uaauaucccg      360

uggguggcua cagaucaggg ccaccaguuc auauuuaugg acucuuaugc cgagaugcug      420

aaggauuuga aacagcauca accccaccuc uucgaaaagu cgucaacucc ugucccacug      480

gacuggacca ucuacuaucu gaggaaaaag gcacucacac aggccauuag caaggaggaa      540

uuagcuugga uccugcuuaa cuuuaaucag aaacggggcu acuaucaguu gcgcggggag      600

gagguggaag auaacaccaa gcgagaggag uucuacgcac ugaaaguagu uaagguggaa      660

cuagacaaag auaauacaag ugccaagaca ugguacaaag uccaccucga aaacggauuu      720

auuuaucccu gcccuuccga caccccaauc gauucuuggg uggguauggu gaaggacuuc      780

auagucacga cuacccugga gaaggacggc agcgagaaaa gaacagcuga uggggaaauu      840

aagagguccu uucgggcccc caaagaggac gauuggacuc uucugaagaa aaagaccgaa      900

ucagaguugg aaaagagugg aaaacaaguu ggcgcguuca ucuacgacac acuccugagc      960

aauccugauc agaagauuau cgguggguua aucaaaacca uugagcguaa guauuacaaa     1020

aaggagcugg aacagauacu cgagacgcaa cagaaguuua ucgcagaacu uucugacaaa     1080

aagcuguucc aggccugugc ugaggaauug uauccacuaa acgauuccca ucgcgagaau     1140

cugcucgaga gagacuucaa acaccuguuu gugcaggaua uuaucuucua ccauaggccg     1200

cuuaagacua aaaagucacu gauaagcaac ugcacauaug aauaccggac cuuuuacaag     1260

aauggagaga aacaaauuag ucccaucaag uguaucgcca aaucuaaccc uuuguuccag     1320

gaauuucgag uauggcaguu cgugcagaac cuccgcauuc ugcaaagaca gagguccgag     1380

ggcgacagca ucaaguauaa ugucgaugug acugcagagu uuuuaaaaga cgaaaacgac     1440

auugcuaauc uguucacaug gcuaaacgag cggaagguua uaaaacagga ucagcuccug     1500

aagucguacu uuaaacuuaa agugaauaag gaaaccaaag aguuggacua ucgcuggaac     1560

uacgucgaug acaagaauaa agaauaucca ugcaacgcca cgagaaccca cauccugucu     1620

uuccucgaga aguguaagga ggaaaagagg auugaggaug accguuuugu guacucccug     1680

uggcaccuuc uguauucagu aguugaaccc aucgaguugc ggggcgcgcu cgccaaguuc     1740

gcagaacgag agcaucugca cccuaguuuu guggaugucu ucgcucaauu cccacccuua     1800

gagaaagaau acgggagcua cuccgagaag gccaucaaaa gacugcuacc gcucaugagg     1860

augggagacu auuggcauga agauaagauu gacgugaaga caaaacagcg cauacagcgg     1920

aucauugaug gugaggcuga cgaugacauc acucacagau cuagggaaaa ggucgagaau     1980

cugcgcagca uuaccgacuu uaaaggccuu ccuuugugga aggccgggua cgugguuuau     2040

aaccgacauu cagagagugg agaacugaca aaauggaaga cuccccacga gcucuccaau     2100

uacauccaga acgaguucaa gcaauauagc cugcggaauc caauagugga acagaucgua     2160

uuagagaccc ugcgugucgu gcacgaucuu uggguuaaac auggcgacuu uucugaaauu     2220

cacuuggagc ucgguagaga uaucaagaac ucaagugaca aaaggaagcg cauggcagaa     2280

cggcagcaag agaaugaaaa cagcagacag agggugcugg agaugcuaau ugaucugaaa     2340

aaggaggccg cuuucgcgga ccucaacccu uacucccccu cucagcagga aaaacugcga     2400

aucgucgagg auggggugcu ucgcucgaau auagaaauuc caaaggacau cgagaagauu     2460

acaaaaagcg caaagcccac cucccgggaa uugacuaaau auagacugug guuagaucaa     2520

aaguacaggu caccuuacac gggccagcca aucagucucg cccagcuguu uacaucuaaa     2580

uaugagauag agcauguaau cccgcaggcu aaguacuucg acgauagcuu uaccaacaag     2640

gugauuugcg aauccgaggu uaauaaagaa aagggaucaa ugcuugccca cgaguucauc     2700

cugaaaaacg gcgggagcac ugucaaugug ggaggucguc aagugcaggu cuugaagaac     2760

gacgcauaug uugaacucau uaaucgccug uacggcaaaa acaaucccgu gaaggccaag     2820

aaacuacugg cugaggauau cgacgaggug guaggggacu uuucugaacg gcagcucaac     2880

gauaccagau auauauccag ggucgugcag aagcugcuua guaauauugu ucgagaggaa     2940

gacgagcaag agagcacauc uaaacacgug aucggauguu ccggccaggu caccacugcc     3000

uugaagaaag auuggggucu gaacgacauc uggaauaaga uuauccagcc ucgguucgaa     3060

cgcuuaaacg agaugacagc aacggaagau uacggggugu ggcaaucacc aaaucauuuu     3120

cagauuagag uucccgagga aauacagaag ggcuucacca aaaagaggau cgaccaccgg     3180

caucacgcga uggaugcucu cgugauugcc ugcgcaacuc gaagucauau caacuauauu     3240

agcaacagau cgggacagga ccgcaaugcc aaauuugaga caaccaggac agaucugcgu     3300

uccaaacuuu guuacaaaga gaacucuagc ggcaauaacu acaauuauau cuucaacaag     3360

ccuugggaca cuuucaccca agaugcucac cgggcccugg aaaauauagu agucucacac     3420

aagcagaacc uaagagugcu cacgaaaacc aauaacuaca cacaguuuau caauaaggac     3480

gggaaaaagg accugcagaa acaagugaag ggagaucauu ucgcagucag gaaaccaaug     3540

caccaggaga cuauuuaugg ucgcguuagu auccgaucca uuaaggaagu ggcuuugaag     3600

aaagcccugg agaacuggaa gcagaucgua gacagaaccu uaaaagauaa gauacgggaa     3660

cucauuggcg cguacgggaa auaugacgcc cagacaaucc ugaaguacuu uaaggauagg     3720

gacuauaaau ucaauaaccu uaagaucaau cgcguggagg ucuacgagua cgaucauaac     3780

ugcgcugcag ugcguaaauc uauuggagac gaucugacua uaaagcaaau cgaaagaauu     3840

accgacgaga gcauccggaa aauuuugaug aaccaccucu ccaauuauga ugacaaccug     3900

aaggaagccu uuucacccga gggccuaaug gaaaugaaua agaacaucaa agagcugaau     3960

ggugggaaga accaucagcc gauauacaaa guuaggcuuu ucgaggaucu cggcagcaag     4020

uuuccucugg gagugaguca caauaaccca gacaaauaug cuaucgccgc aaagggcacg     4080

aauuuguucu uugggauuua cgaagaccag gagggaaagc gcauguucga uucuaucccc     4140

cugaacauug ucaucgaccg auuaaaagaa aagaaauccc cugugcccga gacauauaau     4200

gagagcaagc ucaaauuuca ccugucgcca aacgaucuug uuuacgugcc uaccgacgag     4260

gaacagaagc aacccgaacu ggucgaugua gaccauuugu cagaugagca gaaaggcaga     4320

guguauaagg cgguuucuuu cacugguaau cgggccuucu uugugaacca cagggucagu     4380

aaggugauac augacuacgu agaguucggg acuaaaaaua agauugaaaa cgaugacgau     4440

aaacgcuccc ucaagcacau cuguuggaaa cugaagguga auagacuagg agagaucacc     4500

gacgucaaga gguga                                                      4515


<210>  3419
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3419
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

gguuccguga augaugacgg cucugagcag cuuguaaaaa uacaggcuuc agggaguaga      120

aucauuccca uggaugcagc caugaucgga gacuucaaca agggcaauag cauuucccaa      180

acagcggaaa ggacucggcu gcgcggugug cgacguuugu cugagagaua cuuacugagg      240

cgggaacgcc uacacagaau ccucgauaua cugggguuuc uuccuuucca uuuugcucag      300

gaccuggaua ggcacggaaa aaucgucaag ggcaaagagc caaaguuggc cuggcggaag      360

aacgaagcag gccaguucga guuuauuuuc caagacucgu uuaaagagau gcucgaagau      420

uucaagcuga auuauccgaa ccugaucacg gacgauaaaa aggugcccua cgacuggacc      480

auuuauuacc uccgcaaaaa gggguuaaca ucaaagauca gcaaagagga acuggccugg      540

auacuuuuga auuuuaacca gaagcgagga uauuaccagc ugagagguga ggaggaagag      600

gaaaauaaag auaagcuagu ugaguucuau gcucucaaag uggucgcagu ggaagacagu      660

ggcgagaaga aagggaagga cauuugguac aacguacauc uggagaaugg auggguuuac      720

aggcggacuu ccaacauguc ucuugauugg gugggcaaga ccaaagaauu uaucgucaca      780

accgaccugg agaaggaugg uacuccuaaa aaggacaaag aagggaaugu gaagcguagc      840

uuccgcgccc caaaggagga ugacuggucc uuggugaaaa agaaaacaga agcugauauu      900

gaccagaccc acaagacggu cggagccuau aucuacgaua cucuccuggg caaccccuca      960

caaaagauca gaggcagguu aguucggacc auugagcgaa aguauuacaa ggaggaacug     1020

aaacucauac uugagaagca gaaagaauuu caucuggagu ugcaggaccg cgcgcuauau     1080

gcagccugca ucgaggaacu guacccuauu aacgaggcuc acagaaauaa cguggggaau     1140

agggauuuca cauaucucuu ccuggaagac auccuuuucu accagcggcc acugaagagu     1200

aaaaagagcu ugauagacaa cugucccuac gaggaaaauc aaggacucga uaaggagacu     1260

ggugagauua aaucuuuccc uguaaagugc aucgccaaau cccacccacu guuucaggaa     1320

uuucgcuuau ggcaguuuau cauuaaccug agaaucuaua ggaaggaccu agcacaagau     1380

guggacguca ccaaugagcu ccugaaaaca gaagaggauu acguggcucu uuucgacugg     1440

uugaacggca agaaagaaau ugaucagaag gccuuucuga aguauccggu uuucgggcuc     1500

aaaaaggaga uagagaauua ccguuggaac uaugugcagg acaaacccua cccuuguaau     1560

gaaacccggu cacugaugcu ugcgcgacug gagaagugcg auaucagcau ugaauuuuug     1620

acgaaagaga acgaagaggc ccucuggcau auccuguaua gugucgagga caagaaugaa     1680

aucgugaagg cauuacagaa auucgcugau aagcacgacc ugaacgccuc uuuuguagau     1740

guuuuccaaa aauuuccacc cuucaagaaa gaguacggau ccuacagcgc uaaggccauu     1800

aagaaacuac ucccucugau gagaaugggc aaguauuggu cggaaaauuc uauagacaaa     1860

gguacuaggg agcgcaucga caagauuauc acaggggaau acgaugagaa aauugagaac     1920

cgggugagag aaaaggcaau ccaucuugcc gacgucuccg auuucaaggg cuugccacug     1980

uggcucgugu guuauauagu cuacggaagg cacucagagg cuaaagaaau cauucagugg     2040

aagagucccg cggacaucga uagcuaucug cgcuccuuua aacagcacuc uuuacgaaac     2100

ccgauugugg agcagaucau aauugagacc cugcggguug ugcgugacau cuggaagcaa     2160

guaggcaaua uugaugaaau ccaugucgag cuugggagag aaaugaaaaa cccugcagac     2220

aagaggaaaa agaugacuca gcagaugagc gagaaugaaa acacaaauuu gcgcauaaag     2280

uaccuccuga ccgaguuccu aaaccccgag uaugaagugg agaauguucg gccauacuca     2340

ccuagucagc aagauauccu gagaauuuac gaagagggag ugcucaacuc cgucagcgac     2400

cugcccgaag agaucucuga uauucuuaaa aaguuuacgg agaccgacuu gaaaaagagg     2460

ccaucacgaa gugaagugcu gcgcuauaaa uuauggcucg agcagaagua ccggagcccc     2520

uauacaggug aaguaauccc ucugggcaaa cuuuucacuc cagccuacga gauagaacac     2580

gugauuccgc aguccuauua cuuugaugac ucuuucucga auaaaguuau cugcgaggcu     2640

gaggucaaca agcugaaaag caauuugcuc gggcaugaau uuaucgugaa gcaccaugga     2700

gagauugugg aacugcccuu cggcaaaaag gucagaaucu uuaccguuga cgaguaugag     2760

caguucguga aggauaacua cuccaggaca cguucaaaaa ugaagaaacu acugauggac     2820

gauauuccug aagaguuugu gacucgccaa cucaaugaca gucgguacau aucuaaggua     2880

gucaaaagcc ugcuuuccaa caucgugaga gaaaagggug aggaagaggc cauuucaaag     2940

aauguuaucc cauguaccgg gggcgugaca gauagguuga aaaaggacug gggaauuaac     3000

gaggucugga acaaaaucau acugccccga uuccagcggu uaaaugaacu caccggcacu     3060

aacaaguuua cgacaaagaa uguggggauc caggagauuc cuaccaugcc acuggaacuu     3120

caaaagggau ucaacaagaa acgcaucgau cacagacacc augcaaugga cgccauuauc     3180

auagcuugcg ccaauaggaa cauuaucaau uaucugaaca auaagagcgc aacugcgaaa     3240

gcugagcuau cucgguacga ucuccagaag augcugugug acaaagccaa gaccgauaac     3300

aaugguaacu auaaaugggu uauccgaaag cccugggcau ccuucacaca ggacaccuac     3360

uuggcccugg aaaauauuau agugaguuuu aagcagaacu uaagaguaau caauaaagcu     3420

acuaaccgcu uccuccacua uaacgaggag ggcaagaaaa uuuuugucaa gcaagggaaa     3480

ggagacaauu gggccaucag gaaaagcaug cauaaggaua caguguucgg cgaagugaac     3540

cugcgucgga uuaaaacggu cgcacuuaau gaggcuauga agaacccgca gucuaucguu     3600

gugaaagacu uuaagagaaa acuguuggaa cucuggaauc uggguuucga ugccaagagg     3660

auaaagaaau acuuugagga caaccgcgaa accugguccg auaucaaucu aucaaagauu     3720

gagguauauu acuucaguaa agacacuaag gaucgauuuu ucgcgacaag aaaaccucug     3780

gacaccagcu uugaucggaa gaagaucgag aacaauauua cagacacugg gauccagaaa     3840

auacuucuca ggcaccugga auugaaggau aacaauccag acauugccuu cucgcccgau     3900

ggcaucgacg agaugaaccg caauaucauu cagcugaaca auggaaaaua ccaccaaccu     3960

aucauaaaga uucguuggua ugaacaggcu gacaaauucg cagugggcca gaccgggaac     4020

aaguccucua aauuugucga ggccgcuaag ggaacgaauu uauucuuugc cguguacgag     4080

agcaacaucc ucgauaagaa aaccaacaca auuaucaaga aaagaaauua ugcaacuaua     4140

ccccugaacg uugcgauuga acggcagaag caaggccuuc caguggcccc ugaggacgaa     4200

aaugguaacg aucccaucuu cguccuguca ccaaaugacu ugguguaccu cccgaccgau     4260

gacgagcugg cuaacgggau cauugcccag ccucuagaua ggggacgcau cuauaaaaug     4320

guuaguuccu cuggcgaaca gugcuuuuuc auuaagcaua uaguggcaaa uguccuggua     4380

gacaaguuug aguucagccc ccuuaacaaa auggagcgag cucucacagg ugaaaugauc     4440

aagaugauuu guaucccaau caaaguggau agacugggca auguuuugga guccucaagc     4500

aguuacaaga aauga                                                      4515


<210>  3420
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3420
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

gguuccguga augaugacgg cucugagcag cuuguaaaaa uacaagcuag cgggaguaga      120

aucauuccca uggaugcagc caugaucgga gacuucaaca agggcaauag cauuucccag      180

acagcggaaa ggacucggcu gcgcggugug cgacguuugu cugagagaua cuuacugagg      240

cgggaacgcc uacacagaau ccucgauaua cugggguuuc uuccuuucca uuuugcucag      300

gaccuggaua ggcacggaaa aaucgucaag ggcaaagagc caaaguuggc cuggcggaag      360

aacgaagcag gccaguucga guuuauuuuc caagacucgu uuaaagagau gcucgaagau      420

uucaagcuga aucauccgaa ccugaucacg gacgauaaaa aggugcccua ugacuggacc      480

auuuacuauc uccgcaaaaa gggguuaaca ucaaagauca gcaaagagga acuggccugg      540

auacuuuuga auuuuaacca gaagcgagga uacuaucagc ugagagguga ggaggaagag      600

gaaaauaaaa acaagcuagu ugaguucuac gcucucaaag uggucgcagu ggaagauagu      660

ggcgagaaga aagggaagga cauuugguau aauguacacc uggagaacgg auggguuuac      720

aggcggacuu ccaauaugcc ucuugacugg gugggcaaga ccaaagaauu uaucgucaca      780

accgaucugg agaaggacgg uacuccaaag aaggauaaag aagggaacgu gaagcguucu      840

uuccgcgccc ccaaggagga cgauuggagc uugcucaaaa agaaaacaga agcugacauu      900

gaucagaccc auaagacggu gggagucuac aucuaugaca cucuguuagg caacccuucc      960

cagaaaauca gaggcaggcu gguucggacc auugagcgaa aguacuauaa ggaggaacuc     1020

aaacaaauac uugagaagca gaaagaauuu caccuggagu ugcaggaucg cgcccuauac     1080

gcggcaugca ucgaggaacu guauccaauu aaugaggccc acagaaacaa uguggggaac     1140

agggacuuca cauaccucuu ccuggaagau auccuuuucu aucaacggcc ccugaagagu     1200

aaaaagucau ugauagacaa uuguccuuac gaggaaaacc agggacucga caaggagacu     1260

ggugagauua aaucauuccc aguaaagugc aucgcuaaau cucauccgcu guuucaggaa     1320

uuucgcuuau ggcaauuuau cgugaaucug agaauuuaca ggaaggaucu agcccaggac     1380

gucgauguga ccaacgagcu ccugaaaaca gaagaggacu auguugcacu uuucgauugg     1440

uugaauggca agaaagaaau cgaccagaag gcuuuucuga aguaccccgu guucgggcuc     1500

aaaaaggaga uugagaacua ucguuggaau uacguccagg auaaaccuua uccauguaac     1560

gaaacccggu cccugaugcu ugcccgacug gagaagugcg acauaucaau cgaauuuuug     1620

acgaaagaga augaagaggc gcucuggcac auucuguaca gcguggagga uaagaacgaa     1680

aucguaaagg ccuuacaaaa auucgcagac aagcaugauc ugaaugcuag uuuuguugac     1740

guguuccaga aauuuccccc uuucaagaaa gaguauggau cuuacuccgc caaggcuauc     1800

aagaaacuac ucccacugau gagaaugggc aaguacugga gcgaaaacuc gauugauaaa     1860

gguacuaggg agcgcauaga caagaucauu acaggggaau augacgagaa aaucgagaac     1920

cgggucagag aaaaggccau ucaccuugca gaugugucug acuucaaggg cuugccccug     1980

uggcucgucu guuacaucgu guauggaagg cacuccgagg ccaaagaaau aacccagugg     2040

aagucaccgg cugauaucga caguuaccug cgcagcuuua aacagcauuc cuuacgaaau     2100

ccuauuguug agcaaaucau uaucgagacu cugcgggugg uacgugauau auggaagcag     2160

gucggcaaca uugacgaaau ccacguggag cuugggagag aaaugaaaaa ucccgcggau     2220

aagaggaaaa agaugacaca gcagaugucu gagaacgaaa auaccaacuu gcgcauuaag     2280

uaucuccuga cggaguuccu aaauccagag uacgaaguug agaacgugcg gccuuauagc     2340

cccucacaac aggacauccu gagaauauac gaagagggag uccucaauag uguguccgau     2400

cugccagaag agaucagcga cauucuuaaa aaguuuaccg agacagauuu gaaaaagagg     2460

cccucucgau cagaaauccu gcgcuacaaa uuauggcucg agcagaagua ucggaguccu     2520

uacacuggug aaguaauucc acugggcaaa cuuuucaccc cggcauauga gaucgaacau     2580

gugauacccc agagcugcua cuuugacgau uccuucucua acaaaguuau uugugaggcc     2640

gaggucaaua agcugaaauc gaacuugcuc gggcacgaau uuaucgugaa gcaucacgga     2700

gagaucgugg aacugccuuu cggcaaaaag gucagaauuu uuacaguuga cgaguaugag     2760

caauucguga aggacaauua cagcaggacu cguuccaaaa ugaagaaacu acugauggau     2820

gacaucccag aagaguuugu gacccgccag cucaacgauu cacgguauau uaguaaggua     2880

gucaaaucuc ugcuuagcaa uauagugaga gaaaagggug aggaagaggc uaucuccaag     2940

aacguuauuc ccugcacagg gggcgugacc gacagguuga aaaaggauug gggaaucaac     3000

gaggucugga auaaaauuau ccugccucga uuccagcggu uaaacgaacu cacuggcacg     3060

aauaaguuua caaccaaaaa cguggggaua caagagaucc caacuaugcc ccuggaacuu     3120

cagaagggau ucaauaagaa acgcauugac cacagacauc acgccaugga ugcaaucauu     3180

aucgccugug cuaacaggaa uauaauuaac uaccugaaua acgagucagc caccgcaaag     3240

gcggaacuaa gccgguacga ccuccagaaa augcugugcg auaaggcuaa aacagacaau     3300

aacgguaauu auaagugggu uauccgaaaa ccgugggccu cuuucaccca ggauacuuac     3360

uuggcacugg agaacaucau uguguccuuu aagcaaaauu uaagaguaau aaacaaggcc     3420

acaaaccgcu uccuccauua uaaugaggaa ggcaaaaaga ucuuugucaa acaggggaag     3480

ggagacaacu gggcuauuag gaaaaguaug cacaaggaca cgguguucgg cgaggugaau     3540

cugcgucgga ucaagaccgu cgcccuuaac gaagcaauga aaaauccuca gagcauuguu     3600

gugaaggauu uuaaaagaaa gcuguuggag cucuggaacc uggguuucga cgcuaaaagg     3660

aucaagaagu acuuugaaga uaaucgcgag acuuggucug acauaaaccu auccaaaauc     3720

gagguauauu acuucucaaa ggauacaaaa gaccgauuuu ucgccaccag aaagccacug     3780

gauacaaguu uugaccggaa aaagauugaa aauaacauca cugauaccgg gauucagaag     3840

auccuucuca ggcaccugga guugaaagac aauaaccccg auauagcguu cagcccugac     3900

ggcauugaug aaaugaaucg caacaucauc caacugaaua acggaaagua ucaucagccc     3960

auuaucaaaa uacguuggua cgagcaggcc gacaaguucg cugugggcca gacggggaau     4020

aaauuuucga aguucgucga ggcagccaaa ggaaccaacu uauuuuucgc uguguacgaa     4080

uccaacauuc ucgacaagaa gacaaauacu aucauuaaaa agagaaacua ugccaccauc     4140

ccacugaaug uugcaauaga gcggcaaaaa cagggccuuc cuguggcgcc cgaagaugag     4200

aacgguaaug acccaauuuu uguccugucu ccgaacgauu ugguguaccu cccuacagac     4260

gaugaacugg ccaaugggau caucgcucag ccccuagaca ggggacgcau uuauaagaug     4320

guuagcugua cuggcaacga gggucacuuc aucccagccc gaauugcaaa ucccauacug     4380

caaaccaucg agcuuggcuc aaacaauaaa gcucagaagg ccuggacgga ugaaaugauu     4440

aaggagaucu gcaugccuau caaaguggac agacucggga acguccugga aaguuccucu     4500

agcuacaaga aauga                                                      4515


<210>  3421
<211>  4521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3421
augaagacca uccugggccu cgaccuuggg cccaacagca uuggaugggc caaaguggag       60

gucgaugacg aagguaauua ccugagaaac auaaaguugg gcuccaggau cauuccuaug      120

acacaggaua uguuagggga cuucgauaaa ggaguuaagg ugucucaaac ugcuauccgg      180

acggacuuuc gcggcaaucg acgucugauu gagagacacc uacucaggcg ggaacgccug      240

cauagaguac uucacgcacu gaaauucuug ccaaagcauu augaggaugc caucgguugg      300

gacaggaacg aaccggcgac cuacgggaag uuugugaaua acucagagcc caaacucgcu      360

ugggagcggc aggaagaugg aaguaugcgc uuccuguuua uggagagcuu caaugaaaug      420

gucgccgacu uugcacagaa ccagccuucc cuggugucug auggcaagaa aauaccacuc      480

gacuggacaa ucuauuacuu acgaaagaaa gcccugacua gacccauuaa gaaggaggag      540

cuugcuugga ucuugcugaa uuucaaccaa aaacggggcu auuaccagcu acggggagaa      600

gaggaagagg auaaucucac caagcguaaa gaauauuaca agcugaaagu ugugggaguc      660

gaggcagacg gugccccuaa gggcgggaaa acaugguaca acauucaccu ugagaaugga      720

uggaccgcuc gcugccaguc gaagauccca cuggacgauu gggugggcaa gguaaaagaa      780

uauauaguua cuacagagua cgaagacgau gguaagaccc ccaaauguga caaggagggg      840

aaagugaaga gaucauuuaa gagcccugaa aaagaugacu ggcaguugau uaagacgagg      900

acugagaaau ucaucgauga caguaacaag accgucggag ccuuuauuua ugauuaccuc      960

cuggagaaac cauucgacaa gauccggggc aaguauaucc gaacaauuga acgcaaauac     1020

uauaagaaug aguuaagaga aauacugagg gagcaaacua aauaccauga aggccuccac     1080

ucccuugagg cgcugaagau cugcgcagag gaauuguaca aaaacaacca gcagcaccag     1140

gaguaucuac ugaagaagga uaugaccuuu cuccugcuua augaccugau uuucuaccaa     1200

cggccguuga aaucuaagaa aagccucauc gccgacuguc ccuaugaagu guacaaguau     1260

gaugacaaac ugacagggaa gaaaggacag aagaagauaa aaugcauugc uaaguccaac     1320

ccuuacuauc aggaguuccg cuuauggcaa uuuaucgcca aucugagacu auucucagug     1380

accgauggua aagaggucac ggcagaauac cucaagacug aggaagacua cguuaggcug     1440

uuuaguuucc uuaacgaucg uaaagacauc aagcaggaga cauuuuugcg gagcuuccug     1500

aagcucaaaa aggaaucuau uggcuccgag aaaaucuauc caauucgaug gaauuacaua     1560

gaggaugcua acaagucaua ucccuguaau gccaccagac augaacugcu ucuggcguuu     1620

gacagggccg auaucccucg cgaguugcuc gacguggaag agagggaaua cagacugugg     1680

cacuuacugu auagcauuga ggcaaaagag gaaacugaga gugcucuaag gaagcucaag     1740

gccaacaagg cuuucgggga ucugaauauc gacgaugccu uuguaaacuc uuuccuuaaa     1800

aucaaaccau uugaaaagga guacggagca uauuccgaaa aagccauuaa gaaguugcug     1860

gcugugaugc gccgaggcag ccucuggaaa gaggcggaca uaugcgaggc aacaaagcag     1920

aauaucgcca aaauucugca gaacgacauc gaugaaaagc ugaaaaagaa aauugaggcu     1980

gccgucucgu cuuucaauaa guuauccgac uuucaagguc uucccaccug guuggcaugu     2040

uacgugguuu acgggcggca uucagaagcc gcucagaucc agugcuggca gaguccggag     2100

gaucucaaga cguauauaaa caaauucaag caacacagcc ugcguaaucc uaucguggag     2160

cagugucuac uggaaacccu cagaacaguc uacgacauuu ggaaagaugc cggccagauc     2220

gacgagauuc acaucgaacu gggaaggaac augaagucca cugcagagca gcgcgcgcgg     2280

aaaaccaagg auauacuuag aaacgaaaau acaaacuuga ggauuaagaa ucuguuagug     2340

gagcucaaaa acgacgauaa gaucgagaau guacgacccu auucuccaag ccaacaggaa     2400

auucugcgca ucuacgagga aggcgcccuu caggagcugu caaaagaaga cgagauguuc     2460

gaggauauaa guaagaucuc caaaauggcu cagccuagcc ccacugaauu gucucgguau     2520

aagcucuggc uggagcaaaa guacagauca ccauauaccg ggaggguuau uccccuaagu     2580

aaacuguuua caaccgcaua ccagaucgaa cauauuaucc cucagagccg uuacuucgac     2640

gauuccuuua acaauaaggu gauaugcgag ucugaaguca acucgcucaa agacaauaug     2700

cugggauaug aguucauuaa ggaguguggu ggccgcaucg ugcacugcac ucuuuugggg     2760

gacgucaaaa ucuuuacgaa ggaagaguac aaggauuucg ugacaaaaca uuauauugac     2820

aacaccuaca agcgggauaa acuguuacuc gaagacaucc cacaagaguu uagcgccaga     2880

cagcugaaug auuccaggua uauuucaaag augguucaga cugugcuuag uaacauagua     2940

cgagcugaga augaacagga ggccaccucu aaauucgucg ugagcugcaa cggagcaauc     3000

acaaaucggc ugaagaagga cuggggccua aacgauguuu gguccucaau uguguguccg     3060

cgcuuuaaaa gacucaacga aaucaccaag acuaaugcuu ucggucacug ggaggacaaa     3120

gccgggaagc augucuuuca aacaacggug ccccuggaau ugcagagggg cuucagcaaa     3180

aagcggauug aucaccgaca ccaugcgaug gacgcccugg uaaucgcuug cgcaucuaga     3240

aacauaguga auuacuuaaa caaugagucc gccaacaguc cugagaaacg cgaagaucuc     3300

aggaagaagc ugugugacga gaaacguaag auccggaaac cauggaagac cuuuacucag     3360

gacgcuagag aagcccuuag cgauguuguc gugucuuuca aaaauuaugu gagggucauu     3420

aacaaggcaa caaauuacua cgagcacuau uccucaagug gaaacaaggu uaugaucagc     3480

cagaaaggca augacaugug ggcgauucgc aagccccugc auaaagaauc gguguucggg     3540

cgagugaacu ugugcagaaa gguaaucguc ucccucaaaa aggcccugga gaauauaccu     3600

gcuauuugug auaagacccu acgggacuac aucaacgccc ugguggagaa acacuuuaau     3660

acaaggcaac uucucgcaca cuucaagucu aucaacuauc gccugaaccg ucagugcguu     3720

gaaaaagugg aggucuggca guuuagcgau gaaaagauug agaugguggc uagacggaaa     3780

ucauuggaca ccaguuucga ugacaagcag auauccucua ucacugauac ggggauucaa     3840

aagauccugu uaaauuaccu ccagaccaaa gacgguaacg ccgaaauugc auuuacacca     3900

gagggcaucg aggaaaugaa uaagaacaua agccuguaua augaugggaa agcccaucag     3960

cccauccuua agguuagggu ggcuacuccg aaaggaauga aguacccugu aggccaaacc     4020

gguaacaaag cgacaaaguu cgucguggcc gagaagggga cuaaucugua uuuugcaauu     4080

uacgaaaacg aguugggcgu gcgcuccuac gacaccaucc cacucaauau ugucguugag     4140

cgacugaaag aaggacuauc acccgugccu caggcuaacg aggccgauau cccccugaag     4200

uucuaucuua gcccaaauga ccucguauac gugccuacga aagaaaacga ggacgaacug     4260

uguagagauc ggauauauaa guuugucgac aguucuggca caaccgccaa uuucgugccc     4320

cacacuuccg cuaacacaau uuacaauguu gauaaagagu augcagagaa gugcuguaac     4380

ggggccgugu uuuugaauga auacggauua ggcagcccac agucgaagaa ccagagggcg     4440

cugaccggug agaugaucaa agaaaucugc uggaagcuca aagucgaccg ccuggggaac     4500

auuaucaagg ugauaaaaug a                                               4521


<210>  3422
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3422
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

gguuccguga augaugacgg cucugagcag cuuguaaaaa uacaagcauc agggaguaga      120

aucauuccca uggaugcagc caugaucgga gacuucaaca agggcaauag cauuucccag      180

acagcggaaa ggacucggcu gcgcggugug cgacguuugu cugagagaua cuuacugagg      240

cgggaacgcc uacacagaau ccucgauaua cugggguuuc uuccuuucca uuuugcucag      300

gaccuggaua ggcacggaaa aaucgucaag ggcaaagagc caaaguuggc cuggcggaag      360

aacgaagcag gccaguucga guuuauuuuc caagacucgu uuaaagagau gcucgaagau      420

uucaagcuga aucauccgaa ccugaucacg gacgauaaaa aggugcccua ugacuggacc      480

auuuacuauc uccgcaaaaa gggguuaaca ucaaagauca gcaaagagga acuggccugg      540

auacuuuuga auuuuaacca gaagcgagga uacuaucagc ugagagguga ggaggaaaag      600

aagaaugaua acguuaugau uaucaauagu aaagugauug acgucgugaa ggcugagaaa      660

gacaagaagu acgaaaaaua uugguacaac guacuacucg agaauggccu gcuuuacagg      720

gcagccuucu auaacgauau cuccuuuugg aaggggcaca cuaaagaguu cguucugaag      780

accaucaaau ugaagucugg aggcacaaag caggaacuca gcuuucugcc uuccuucgac      840

gagauugaga aaauggaaaa gaaacaaaag gauaaaaugu acuucaagau aaaguuaaaa      900

accgaagcug acaucgauca ggcccauaag acugugggua cauauauuua cgacacccug      960

cucgggaacc caucacagaa aauccgggga cgucuugucc gcacgauaga gagaaaguau     1020

uacaaagagg aacugaaguu gauucuagau aagcagaaag aguuucaccc cgaacugcaa     1080

gacagggcgc ucuaugcagc cugcaucgag gaacuguacc cuaucaauga ggcucaccgg     1140

aacaaugugg gcaaccgaga uuucacuuac cuuuuucugg aagacauuuu guucuaucag     1200

cgcccacuca agaguaaaaa gagccugauc gauaauuguc ccuacgagga gaaccagggc     1260

uuagacaaag aaaccgggga gauuaagucu uuuccuguga aaugcauagc caagucccau     1320

ccacuguucc aagaauuuag acuauggcag uucaucguca aucucaggau uuaucggaag     1380

gaccuggcac aggauguuga cgugacaaac gagcuuuuga aaacugagga agauuacgua     1440

gcucuguuug acuggcucaa uggaaagaaa gagaucgauc agaaggccuu ccugaaauau     1500

ccgguguuug gucuuaagaa ggaaaucgag aacuaccgcu ggaauuaugu ccaagacaaa     1560

cccuacccuu guaacgaaac cagacuguug augcucgcga ggcuggagaa gugcgauauu     1620

ucaauagagu ucuuaacaaa agaaaaugag gaagcccugu ggcacauccu auacagcgug     1680

gaggacaaga acgaaauugu uaaagcacuc cagaaguuug cugauaagca ugaccugaau     1740

gccaguuucg uggaugucuu ccguaaauuu ccacccuuca agaaagagua uggcucuuac     1800

uccgcuaagg ccaucaaaaa guugcuuccu cugaugcgga uggggaagua uuggagcgag     1860

gacucgauug auaaaggaac ccgaaagaga auggacaaaa ucauaacggg cgaauacgac     1920

gagaagaucg aaaacagggu gcgcgagaaa guaauucacc ucgcagaugu uucugacuuu     1980

cggggucugc cauuauggcu gguguguuau aucgucuacg ggagacacuc cgaagccaag     2040

gagauuacuc aguggaaauc acccgcugau aucgacaguu aucuuaagag cuucaagcag     2100

cauuccuuga ggaacccgau aguggagcaa auuaucauug aaacacuccg cgucgugcga     2160

gauaucugga aacagguugg caauauagac gagauccacg uggaacuggg acgggagaug     2220

aagaacccug cggauaaacg uaagaaaaug acccagcaga ugucugagaa ugaaaacacu     2280

aaucuaagaa uuaaguaccu gcucacagag uuucugaacc ccgaauacga gguagaaaau     2340

gucaggccau auagcccuuc acaacaggac auccuucgca uuuacgagga gggcguguug     2400

aacaguguuu ccgaucugcc cgaagagauc agcgacauau uaaagaaauu caccgaaacg     2460

gaucucaaga aacggccauc uagaucagag auucugaggu auaaacuuug gcuggaacag     2520

aaauaccgaa gucccuauac cggggaggug aucccuuugg gaaagcucuu uacaccagca     2580

uacgagaucg aacaugucau uccgcagagc ugcuauuucg acgauuccuu uucuaauaag     2640

gugaucugug aggccgaagu aaacaaacug aagucgaauc uacuggguca cgaguucauu     2700

gugaaacauc acggcgaaau aguugagcuc cccuuuggga agaaaguccg caucuucacu     2760

guggacgagu acgaacaauu ugugaaggac aacuacagcc ggaccagagg aaagaugaaa     2820

aagcugcuua uggaugacau uccugaggag uucgucgcua ggcaguugaa ugauucccgu     2880

uauaucucaa aaguugugaa gagucuguua ucuaacauug ugcgcgaaaa aggcgaggaa     2940

gaggccauca gcaagaaugu aauaccaugc acaggugggg ucacugaccg gcucaagaaa     3000

gauuggggca ucaacgaagu guggaacaag auuauccugc ccagauucca gaggcuuaau     3060

gagcugaccg gaacaaacaa auuuaccacu aagaauguug gcauucaaga aaucccuacg     3120

augccacuag agcuccagaa aggguucaac aagaaacgaa uagaccaccg gcaucacgca     3180

auggaugcca uuaucaucgc uugugccaau cgcaacauua uaaauuaccu gaacaaugag     3240

uccgcaacag cgaaggcuga auugucaaga uaugaccugc agaaguuacu cugcgauaaa     3300

gccaagaccg acaacaaugg aaacuacaaa ugggugauca ggaagcccug ggcuagcuuu     3360

acucaggaua ccuaucuggc ccuugagaau auuaucgucu cuuucaaaca aaaccugcgg     3420

gugauuaaua aggcuacaaa ccgauuuuug cauuacaacg aagaggguaa gaaaaucuuc     3480

guuaagcagg gcaaagggga caauugggcc auaagaaagu ccaugcacaa agacaccgug     3540

uuuggagaag uaaaccuccg caggaucaag acugucgcac ugaaugaggc uaugaagaac     3600

ccgcagagua uuguggugaa agauuucaag cguaaacuac uggagcuuug gaaucucggc     3660

uuugacgcca agcggaucaa aaaguauuuc gaagauaaca gagagacaug gagcgacauu     3720

aaucugucua agaucgaagu cuacuacuuu uccaaagaua cgaaggacag guucuucgcg     3780

acccgcaaac cuuuggauac uucauuugac cgaaagaaaa uagagaacaa uauuacagau     3840

accgguaucc agaagauccu guuaagacac cucgaacuga aagacaacaa uccagauauu     3900

gccuucaguc ccgacgggau cgaugagaug aaccggaaua uaauucaacu uaacaauggc     3960

aaguaucauc agccuaucau uaagaucagg ugguacgagc aggcugacaa auuugcaguu     4020

ggacagacag gcaacaaguu cagcaaauuu guggaagccg cuaaggggac uaaucuguuc     4080

uuugccguau augagucgaa cauauuggac aaaaagacca acacgauuau caagaaacgc     4140

aauuacgcaa ccaucccccu caacguggcg auugaacguc aaaagcaggg acugccaguc     4200

gccccugagg augagaaugg caacgacccc aucuucgugc uauccccaaa ugaucugguu     4260

uaucuuccga cagacgauga acucgcuaac gguauuauag cccagccucu ggacagaggg     4320

cggaucuaca aaaugguguc uuguacugga aaugagggcc acuuuauucc cgcaaggauc     4380

gcuaacccaa ucuugcaaac cauugaauua gguagcaaua acaaggccca gaaagcaugg     4440

acagaugaga ugauaaagga aaucugcaug cccauuaagg ucgaccgccu gggcaaugug     4500

cucgagucaa guuccucuua uaaaaaguga                                      4530


<210>  3423
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3423
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugguc ccugauagag       60

cacgauuuca aaaauaagca gggucagauc gaaggcuugg gggugaggau uauccccaug      120

ucucaggaga uuuuaggaaa auuugacgcc ggccagucaa ucagucagac agcugaucgg      180

acuaaguacc gcgguguccg acgucuguau caaagagaca accuaaggcg ggaacgccuc      240

cauagaguuc ugaaaauacu ugauuuccug ccuaagcacu acagcgaguc caucgacuuu      300

caggauaagg uggggcaguu caaaccaaag caggaaguaa aauugaauua uaggaagaac      360

gagaaaaaua agcaugaguu uguguucaug aacucuuuua uugaaauggu cucggaguuc      420

aagaaugcac aaccggaacu cuuuuacaac aaaggaaaug gcgaggagac gaagaucccc      480

uaugacugga cccuguacua ucugcggaaa aaggcccuca cacagcagau uacuaaagaa      540

gaguuagcgu ggcugauccu uaacuucaau cagaagcgcg gcuacuacca auugcgaggg      600

gaagauauag acgaggauaa aaacaagaag uauaugcagc ugaaagugaa uaaccuaauu      660

gacucaggag cuaagguuaa agguaaggug cucuacaaug ucaucuuuga uaacggcugg      720

aaauaugaaa agcagauugu gaacaaggac gagugggagg ggagaaccaa agaauuuauc      780

aucacaacca agacucugaa aaauggaaac auuaagagga cauacaaagc cguagacagc      840

gagauagauu gggcagccau caaggcuaag accgaacaag acauuaauaa agcaaacaag      900

acgguuggcg aguauaucua cgaaagucuu cuggauaauc cuucccagaa aauacggggu      960

aaguugguga aaacuauuga gcguaaguuc uauaaggagg aauuugagaa acuccugucu     1020

aagcagaucg aauuacagcc agagcuguuc aacgaaagcc ucuacaaagc cugcaucaag     1080

gagcuuuacc cccgcaauga gaaccaccaa uccaauaaca aaaagcaggg guucgaauau     1140

cuguucaccg aggacauuau cuuuuaccag agaccuuuga aaucacagaa gaguaauauu     1200

agcggauguc aauucgaaca uaagauauau aaacagaaga acaaaaagac aggcaaacua     1260

gagcugauca aggagccaau uaagacuauc ucuagguccc acccccucuu ucaggaguuc     1320

cggaucuggc aguggcugca aaaucuuaaa auuuacaaca aggagaaaau agaaaauggc     1380

aagcuggagg augucaccac acaguugcuc ccuaacaacg aggcuuaugu gacccuguuu     1440

gacuucuuaa auacgaaaaa ggaacuggag cagaagcagu uuaucgaaua cuucgugaaa     1500

aagaaacuaa uugauaagaa agagaaggaa cacuuucgau ggaacuucgu cgaggacaag     1560

aaauauccau uuucagaaac ucgcgcccaa uuccucagca gacuggcgaa gguuaaaggg     1620

aucaagaaua cagaggauuu ccuuaacaaa aauacccagg ugggaaguaa ggagaacucu     1680

ccguuuauua aaaggaucga acaguugugg cauauaaucu acuccguaag cgaccugaag     1740

gaguacgaaa aggcacucga gaaauucgcc gaaaagcaca aucuggagaa agauucguuu     1800

cuuaagaacu ucaaaaaguu ucccccuuuc gugucugacu augcuuccua cucaaagaaa     1860

gccauuagua agcuguugcc aaucaugcgg auggguaaau auuggagcga guccgcaguc     1920

cccacucaag ugaaggaacg cucucucagc auuauggaga gaguuaaagu gcugccuuua     1980

aaggaaggcu acucagacaa ggaucuggcu gaccuacuca guagggucuc cgaugacgau     2040

aucccaaaac agcugauaaa gagcuuuauu ucuuucaaag acaagaaucc ccuuaaaggg     2100

uugaacacau aucaggccaa uuaccuggug uauggacguc auucagagac cggcgauauc     2160

cagcacugga agacgccgga ggacauugau cgguaccuca acaauuuuaa gcaacacagu     2220

cugcgaaacc cuaucguaga acagguugug auggagaccu uaagagucgu gagggacaua     2280

ugggaacauu acgguaauaa cgagaaagau uucuuuaagg aaauccacgu cgagcugggg     2340

cgcgagauga aaagccccgc gggcaagcgg gaaaaacuuu cccagagaaa cacagagaau     2400

gaaaacacua aucauaggau ucgcgaggug uugaaggaac ucaugaacga cgccucuguu     2460

gagggaggcg ugcgagauua uucgccaagc cagcaagaga uccugaagcu auacgaagag     2520

gggauuuauc agaauccuaa caccaauuac cugaaaguag acgaagacga gauccucaag     2580

auacggaaaa agaacaaucc cacacagaaa gaaauucagc guuauaagcu guggcuugag     2640

caaggauaca ucuccccaua uacugguaaa aucauucccu ugaccaagcu guucacacac     2700

gaguaccaga ucgaacauau uauaccucag ucaagauacu augauaacag uuuaggcaau     2760

aagaucauuu gcgagucuga agucaacgag gacaaagaua auaagaccgc auacgaguau     2820

cucaaagugg aaaaggggag caucguuuuu ggacacaaac ugcuuaaccu ggacgaguac     2880

gaagcucacg ugaauaagua uuucaagaaa aacaagacua aauugaagaa ccuccugucc     2940

gaggauauuc cagaaggcuu caucaauagg caacuaaacg acucacgcua cauaagcaaa     3000

cuggucaagg gucuccuguc uaauaucgug cgggagaacg gggagcagga agccacgucc     3060

aagaaucuua uuccgguaac aggcgugguu accaguaaau ugaagcagga uuggggacug     3120

aacgacaaau ggaaugagau cauugcuccc agauuuaaga gguuaaacaa acucacuaau     3180

agcaacgauu ucggcuuuug ggacaaugau aucaacgccu uccgaauaca ggucccugac     3240

ucucugauua agggguuuuc caagaaacgc aucgaucauc ggcaccaugc acuugacgcc     3300

cugguggugg cuuguaccuc aagaaaucac acacacuauc uaagugcgcu caacgcagaa     3360

aauaagaacu acagccugag ggacaaauug gucaucaaga augagaacgg agauuacacc     3420

aaaacuuucc aaauuccaug gcaggguuuu acaauagaag ccaagaacaa ucuggagaaa     3480

acgguugugu cguucaagaa gaacuuacgu gugaucaaua aaaccaacaa uaaguuuugg     3540

uccuauaaag acgagaacgg caaucucaac cuggggaagg auggaaaacc caagaagaaa     3600

cuucgcaagc agacuaaagg cuacaauugg gcuauucgga agccucugca uaaagaaaca     3660

guaucuggua ucuauaacau uaaugcccca aagaacaaga ucgcaaccag cgucagaaca     3720

uugcucacug agauaaagaa ugaaaagcac cuggccaaaa ucaccgaccu aaggauucga     3780

gagacgaucc ugcccaacca ucuuaagcac uaccucaaua acaaagggga agcuaacuuc     3840

ucagaggccu uuagucaggg cggaauugag gaucugaaua agaagaucac cacauugaac     3900

gaaggcaaaa agcaucaacc gauauaucgg gugaaaauuu ucgagguugg guccaaguuc     3960

ucuaucagcg aagacgaaaa uuccgcaaaa ucaaagaaau acguggaggc ggcuaaggga     4020

acuaaccugu uuuucgccau cuauuuagau gaagagaaua agaaacgcaa cuacgaaacc     4080

auuccucuca augaggucau cacacaccag aaacaggugg caggcuuucc aaaaagcgaa     4140

agacugagug uucagcccga cucucaaaag gguacuuucc uuuuuacccu guccccuaac     4200

gauuuggugu acguacccaa uaacgaggag cucgaaaaua gggaccuguu caaccuaggg     4260

aaucugaacg ucgagcagau aagccggauu uauaaauuua cggauucguc agacaagaca     4320

ugcaauuuca ucccauuuca ggugucuaaa cuuauuuuca accucaagaa gaaagaacag     4380

aagaaacugg acguggauuu uaucauacaa aacgaguucg gauugggcag uccucagucc     4440

aagaaucaaa agagcauuga cgaugucaug aucaaagaaa aguguaucaa auuaaagauu     4500

gaccgacugg guaacaucuc uaaagccuga                                      4530


<210>  3424
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3424
augaagacca uccugggccu cgaccuuggg acaaacagcg ugggaugggc cgucauuaau       60

uccguuauaa aagagcaggc ugaaagaauc uggauugaga uggcagguuc uaggaucauu      120

cccauggaug ccgcggugcu gggcgacuuc gauaagggga acucaaucag ucaaacuagc      180

gaacggacgc gcuugcgagg aguacguaga uuaagggagc ggcagcugcu acgcagagaa      240

aggcuccaca aagugcugaa uauacuuggc uuucugccua accauuacuu ggaguauauc      300

gacuucgaga agcacccagg uaaauuucuc uccgaugcug aaccgaagcu gcccuggguc      360

aaggacgagc aggggaaaua caccuuccug uuucaggccg cauucgauga aaugcucgcc      420

gacuuugcua uucaucaacc uucuuuagug ucgggagaua agaaaauccc auaugacugg      480

acaauuuacu aucugcggaa gaaagcacuu acugagauga ucucaaagga ggaauuggcc      540

uggauacugc uaaauuucaa ccagaagcgc ggcuacuauc agcuccgagg cgaggaagag      600

gaagauaaug cugggaaaag cguugaguuu uacgcccuga aggugcuuac cgucgaggcg      660

acagacgaaa agaagggaag agaugugugg uacaacguac accuggagaa ugguuggauu      720

uauaggcgga guuccucugu ucccuuggac ugggaaggcc agaucaaaga guucauugug      780

accacugacc ucaacccuga uagcacacca aagcuggaca aauacgggga agucaagcgu      840

uccuuucgcg cacccaagga ggaugacugg auguuaauca aaaagagaac cgaggccgau      900

aucgcuaaua cgcauaaaac ugugggagcc uauauuuacg acacccugcu ccaaaagccu      960

cagcagaaaa uaaucggcaa acuugugaag acagucgaaa ggaaacugua ucgggaugag     1020

uugucacuaa uucugcagaa gcaaugcgca uuccacgcug aacuccagga ccgaacucug     1080

uacgcccgcu guguugaggc gcuuuaucca aacaaugaag ugcagagaag gaacaucgcc     1140

aaucgggauu uuguauaccu guugauggac gauguccucu ucuaccaacg cccgcugaaa     1200

accaagaaaa guuuaauagc aaacugcccc uaugaggaga acgaauacgu ggacagagag     1260

acaggugaaa uuaagaaggc uccucugaaa uguaucgcca agagccaccc acuauuccag     1320

gaguuuaggc ucuggaauuu ccugucuaac auccguauuu aucagaaaga gaaggaaauc     1380

aaugggaccc uucauuugga cguggauguu acggcugagu uucugaaaac ugaagacgau     1440

uacguggccc ucuucgacug gcugaacucc cggaaggaga uugaacagaa gacauuucuu     1500

aaauauccca aguucggacu gaaaaaggag auaucaaauu accgauggaa cuaugucgag     1560

gauaaacaau acccuugcaa ugaaaccaga cacgcaaucu ugagcaggcu cgagaaggcc     1620

aacgugugua uugacuuucu gaguaaugaa aaagagguag cuuuauggca uauccuguac     1680

ucuguuuccg auaagcagga aaucgagaag gcgcuacgca cuuucgcagc caaaaacggc     1740

cucgacgaga gcuuugugga agcauucaag aaauuuccac ccuucaaguc ggaguauggc     1800

gccuacucug caaaagccau uaagaagcug cuuccguuga ugcggugcgg gaaauauugg     1860

gaugucuccu caauagacga uacaaccaag gaaagaauca aaaagauugu ggcuggagag     1920

uacgacgaaa ggaucgccgu caaagugcgc gauaagguua ugaaucugag ugacauuagc     1980

uguuuuaagc gacucccucu gugguuagca ugcuauaucg uguacaaccg gcacuccgag     2040

gacaaagagg cgguaaagug ggaaucuccc gcugauauag acgccuaucu ggcuagcuuc     2100

aaacagcacu cacuucguaa uccaaucguc gaacagguga uuacggaaac cuugagaguu     2160

gugagggaua ucuggaagca agucggucgc auugacgaga uccauauaga gcucggccgg     2220

gaaaugaaaa acccugccga uaagagaaag aggaugacag cucagauucu ggagaaugaa     2280

aacgccaacc uacgaaucaa agcacugcuc gcugaguuca ugaaucccga auacgagauu     2340

gagaacgugc gcccauacag ucccucccag caggaaaucc ugcggauaua ugaggacaug     2400

guacuucacg ccgaagauca auugccugag gaaaucgacg cgauucugaa gaaauuuaau     2460

agcucuaagg ugccaacuaa aucagaguuc uuaagauaca agcucuggcu ggagcagaaa     2520

uauaggaguc cguacaccgg ggaacuuauc ccccugggaa aguuguuuac agccgcuuau     2580

gagauugaac auguuauccc ucagagccgu uacuucgaug acuccuuuuc uaacaagguc     2640

auaugugagu cggcagugaa uaaacucaag gauaaccagc ugggcuauga guucauuaaa     2700

aaucaccaug gucaaaaggu ggaggucggg uuuggcaaaa gcguugagau cuucuccgug     2760

gacucauacg aacgcuuugu gaaggagcag uacgccaaga gugcuguaaa aaugaagaaa     2820

augcuaaugg augacauccc agaacaguuc auugagcggc aacugaacga cucuagauau     2880

aucagcaagg ucauuaaagg acuccugucc aauauaguga gggagaagaa cgauucaggc     2940

gaauacgagc ccgaagccgu uagcaagaau aucguggucu gcacuggguc ugugaccgac     3000

cgacuuaaac gggauugggg aaugaacgac guuuggaacu ccauugugua uccucgcuuu     3060

gagagauuga auagucugac agauacccag agcuucgguc acugggaaaa uaagucuggc     3120

aaaaagguau uucagacuga guuaccacuc gaguaccaga aaggguucuc caagaagagg     3180

aucgaccacc ggcaucacgc aauggaugcg auugucaucg ccugugcuac gcgaaaccau     3240

gugaacuauc ugucaaauga aagugccagc agaaacgcaa aaauaucgcg cuacgaccuu     3300

caaaggcugc uaugcgauaa gucccguaca gacgauaaug gaaacuauaa augggugauc     3360

aagaaacccu gggacaccuu cacucaggac gcucgggagg uccucucuaa gauuguugug     3420

agccugaagc agaauuugag aaucauuaac aaaaccacaa auguauacag gcaccuggau     3480

gccaccggca accgcgugua cgaaaagcag aaaacuggug acucaugggc aauccgaaag     3540

ccgaugcaca aagauacagu cuuuggggcc gugaauuuaa gaaagguuaa agugguccgg     3600

cucagugugg cucuggacgc gccuacgaug auaguugaua agagggugaa gaacaaaguc     3660

uucgagcuuc uguccuauaa guacgacaaa aagcgcauug aaaaauauuu uaaggagaau     3720

gccguauugu ggaaggagcu cgaucuggca cguguggcug uuuacuauuu caccgaagcc     3780

acuacagagc cccuaguggc cgucagaaaa ccacuggacu cuaccuuuaa cgaaaagaaa     3840

aucaaggaga gcgugacaga uacuggcauc cagaaaauuc uucucaauca ucugugcacc     3900

aaggaaggaa agccugacuu ggcuuucucc gcagagggca uagaggaaau gaaccggaau     3960

cuguuacaac ucaacaacgg gaaaaggcac cagcccaucu acaagguacg cguguaugag     4020

ccacgaggaa auaaauuuuc agucgguguu cugggcaaca aggggacgaa auggguggaa     4080

gccgcgaagg gaaccaaucu uuucuuugcu auuuacauca cagaggaugg caagagaacu     4140

uacgagacca uuccucugaa cauggugauc gaacgggaga aacaggguuu ggcccccguc     4200

ccagacaaga augaaaaagg ggauaagcuc cuguucuggc uaagcccgaa cgaccuggug     4260

uaucuuccua cagaggagga acucgaguuu ggcaggauaa augaacccau cgaccgcgga     4320

agaauuuaca aaaugguuag uucuuccggc aaccaauguu ucuucauccc acagagcauu     4380

ucgaaugcaa ucauacccac uaaggagcug ggggccaaca auaaagcuga aaggucaugg     4440

gauggagcaa ugauuaagaa caucugcauc ccuguaaagg uggaccgguu gggccguauu     4500

aucgagguca aauaucgcau aaaugaauga                                      4530


<210>  3425
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3425
augaagacca uccugggccu cgaccuuggg acaaacagcg ugggaugggc cgucauuaau       60

uccguuauaa aagagcaggc ugaaagaauc uggauugaga uggcagguuc uaggaucauu      120

cccauggaug ccgcggugcu gggcgacuuc gauaagggga acucaaucag ucaaacuagc      180

gaacggacgc gcuugcgagg agcucguaga uuaagggagc ggcagcugcu acgcagagaa      240

aggcuccaca aaguacugaa uauacuuggc uuucugccua accauuacuu ggaguauauc      300

gacuucgaga agcacccagg uaaauuucuc gccgaugcag aaccgaagcu ggccugggug      360

aaggacgagc aggggaaaua caccuuccug uuucaggcug cauucgauga aaugcucgcc      420

gacuuugcua uucaucaacc cuccuuaguc ucuggagaua agaaaauccc uuaugacugg      480

acaauuuacu aucugcggaa gaaagcccuu acugagauga ucucgaagga ggaauuggcg      540

uggauacugc uaaauuucaa ccagaagcgc ggcuacuauc agcuccgagg cgaggaagag      600

gaagauaaug cagggaaauc aguggaguuu uacgcccuga agguucuuac cguggaggcu      660

acagacgaaa agaagggaag agaugucugg uacaacgugc accuggagaa ugguuggauu      720

uauaggcgga gcaguuccgu accauuggac ugggaaggcc agguuaaaga guucaucgug      780

accacugacc ucaaccccga ugggacaccu aagcuggaca aauacggaga agucaagcgu      840

ucuuuucgcg ccccaaagga ggaugacugg auguuaauua aaaagagaac cgaggcagau      900

aucgcuaaua cgcauaaaac ugugggcgcc uauaucuacg acacccugcu ccaaaagccc      960

cagcagaaaa uuauagguaa acuugugaag acagucgaaa ggaaacugua ucgggaugag     1020

uugagccuaa uccugcagaa gcaaugcgcg uuccacgccg aacuccagga ccgagcacug     1080

uacgcucgcu guguugaggc ccuuuauccu uccaacgaag ugagaaggaa aaauauugcu     1140

aaccgggauu uuguauaccu guugauggac gauauccucu ucuaccagcg cccacugaag     1200

acuaaaaagu cauuaauagc caauugcccg uaugaggaga acgaauacgu ggacagagag     1260

accggggaaa uuaagaaaac accccugaag uguaucgcaa aaagucaccc ucuauuccaa     1320

gaguuuaggc ucuggaacuu ccugagcaau auccguauuu aucagaagga gaaagaaauc     1380

aacggaaccc uucauuugga cgucgaugug acggccgagu uucugaagac ugaagacgau     1440

uacguugcuc ucuucgacug gcuuaauucu cggaaggaga uugaacagaa aacauuucuu     1500

aaguauccaa aauucggccu gaagaaagag auauccaacu accgauggaa uuauguggag     1560

gauaagcagu accccugcaa cgaaaccaga cacgcgaucu ugucaagccu cgagaaagca     1620

aaugucugua uugacuuucu gaguaacgaa aaggaggugg ccuuauggca uauccuguac     1680

ucuguauccg auaagcaaga aaucgagaaa gcucuaagga cuuucgccgc aaagaauggc     1740

cucgacgaga gcuuuguuga agccuucaaa aaguuuccuc cauucaaauc ggaguauggg     1800

gcuuacucug ccaaggcaau uaagaaaaug cugccccuua ugcgcugcgg aaaguauugg     1860

gauguguccu caauagacga uacaaccaaa gaacggauca agaaaauugu cacgggugag     1920

uacgacgaaa gaaucagugu gaaggucagg gauaagguga ugaacuugag cgacauuucc     1980

uguuuucgcc gacugccgcu cuggcuggcg ugcuauaucg uuuacaaucg gcacucugag     2040

gacaaagagg cugugaagug ggaaagcccu gccgauauag acgcauauuu agccucauuc     2100

aaacagcaca gucugcguaa ccccaucgua gagcagguca uuaccgaaac acuuagagug     2160

guuagggaua ucuggaaaca ggugggccgc auugacgaga uccauauaga guuggggcgg     2220

gaaaugaaaa auccagcuga uaagagaaag aggaugacug cccaaauucu cgagaacgaa     2280

aaugcaaacc ugcgaaucaa agcucuacug gccgaguuca ugaacccuga auacgagauu     2340

gagaaugucc gccccuacuc cccaagccag caggaaaucc uccggauaua ugaggacacc     2400

gugcugcacg cggaagauca gcuucccgag gaaaucgacg ccauuuugaa gaaauuuaac     2460

ucuucaaagg uaccuacaaa aagugaguuc cugagauaca aguuauggcu cgagcaaaaa     2520

uauaggagcc cauacacugg agaacugauc ccgcuuggca agcuguuuac cgcugcauau     2580

gagauugaac augugauccc ccagucccgu uacuucgaug acucuuuuuc gaauaagguu     2640

auaugugaga gcgccgucaa caaauugaag gauaaucagc ucgguuauga guucauuaaa     2700

aaccaccaug ggcagaaggu ggaagugggc uuuggaaaau ccgucgagau cuucucaguu     2760

gacaguuacg aacgcuuugu gaaggagcaa uacgcuaagu cugccgugaa aaugaagaaa     2820

cugcuaaugg augacauccc ugaacaguuc auugagcggc agcugaauga cagcagauau     2880

aucuccaagg uaauuaaagg ccuccuguca aacauaguca gggagaagaa ugauagcggg     2940

gaauacgagc cagaagcagu gucuaagaac aucguugugu gcacaggauc cgucaccgac     3000

cgacuuaaac gggauugggg uaugaaugac guguggaaca guauuguuua uccccgcuuu     3060

gagagauuga auagccugac ugauacgcaa ucuuucggcc acugggaaaa caaguccggg     3120

aaaaaggugu uucagacaga guuaccucuc gaguaccaga aaggauucuc aaagaagagg     3180

aucgaccacc ggcaucacgc gauggaugcc auuguaaucg cuugugccac ccgaaaucau     3240

gucaacuauc ugaguaacga aagcgcaucg agaaaugcua aaauaucccg cuacgaccuu     3300

cagaggcugc uaugcgauaa gaaccguacu gacgauaaug gcaacuauaa augggugauc     3360

aagaaaccau gggacaccuu cacacaagac gcccgggagg ugcucucuaa gauugucguu     3420

agccugaagc agaauuugag aaucauuaac aaaaccacua auguguacag gcaccuggau     3480

gcaacaggua accgcguaua cgaaaagcag aaaacggggg acucaugggc cauccgaaag     3540

cccaugcaca aagauaccgu guuuggcgcu gucaauuuaa gaaaggugaa aguugugcgg     3600

cucagugucg cgcuggacac uccggccaug auaguggaua agaggguuaa gaacaaagug     3660

uucgagcuuc uguccuauaa guacgacaaa aagcgcauug aaaaauauuu uaaggagaau     3720

gcauucuugu ggaaggagcu cgaucuggcu aaagucgccg uauacuauuu uacagaagcc     3780

accacagagc cucuaguggc uguucguaag ccacuggacu cuacuuucaa cgaaaagaag     3840

aucaaagaga gcgugaccga uacgggaauc cagaagauuc uucucaauca ucugugcacc     3900

aaggaaggca aacccgacuu ggcauuuucc gccgagggga uagaggaaau gaacagaaau     3960

cuguuacaac ucaacaacgg aaagucacac cagccuaucu acaaaguccg gguguaugag     4020

cccaggggua auaaguucag cguaggcgug cuggggaaca aaggaacaaa gugggucgaa     4080

gcggcuaagg gcacuaaucu guuuuucgcc auuuacauca ccgaggaugg uaaacgcaca     4140

uacgagacua uuccacugaa cguugugauc gaacgagaga agcagggguu ggugccuguc     4200

cccgacagaa auaaaaaggg cgauaaacuc cuguuuuggc uaaguccaaa cgaccuggug     4260

uaucuuccga ccgaagagga acgggaguuc ggaaggauaa augagccuau cgaccgcggc     4320

agaauuuaca agaugguuuc uuguacgggg aacgaaggac auuucauccc cgcaaaugua     4380

gccaacccaa uugugcccgu caucgagcuc ggcuccaaua acaaagcuca gagggcaugg     4440

acaaaugaaa ugauaaagga uauuugcauc ccuaucaagg uggaccggcu gggucacauu     4500

aucgagguua aauaucguau agaugaauga                                      4530


<210>  3426
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3426
augaagaaaa uccugggccu cgaccuuggg accaacagcg ugggaugggc cgucauuaau       60

uccguuguga acgaugaggg uaaggaacag cugguaaaua uaucuucaug cggcaguaga      120

aucauuccca uggacgcugc aacauugggg gauuucgaca aaggaaacag caaguccccu      180

guggccgaga ggacucgguu acgcggcguc cgacgucugc uagaaagauc ucuccugagg      240

cgggagcgcc uucacagagu gcugucgguu augggguuuu ugccagaaca uuacgcggcu      300

caacucgaua gguaugggaa auuccugccg gagacggagc ccaagcuggc cugguacaag      360

gacgaaaccg gaaaauaucg guuucucuuc caggauucau uuaaugagau guuagaagac      420

uuccacgagc aucacccuga gcugguggca gaaggcaaga aaauccauua cgauuggaca      480

auuuauuacc uucgcaagaa agccuugagc cagaagauca cuaaggagga acuggcuugg      540

auacuacuca acuuuaauca gaaacgaggc uauuaccaac ugagagggga ggaggaacag      600

gaggacaaca agaaaagugu cgaauacuau gcacuuaagg ugguagccgu ugaggauucc      660

ggagacagga aagguaagga uaucugguac aaugugaaac uggaaaacgg cugggucuau      720

cggcgugcuu cuggggugcc auuggacugg gugggaaagg ucaaggaguu cauuguuacc      780

acagaccucg augaaaaagg caaucccaag aaagacaagg augguaacgu gaaacgcagc      840

uuuagagccc cuaaggagga cgauuggggg cuguuaaaga ccaggacuca ggcgcagauc      900

gacgaguccg gacaaacagu aggcaccuac auuuaugaau cacugcuccg gaugccagau      960

cagaaaaucc gaggcaaacu ugugcgcacg auagagagaa aauacuauaa ggacgaacug     1020

aggaaaauuu ugaucaagca gagugaguuc cacacugcac uacaggauca caaucugcuc     1080

cuggccugua uugaggaacu uuaccccaac aaugaggcuc aucggcgccu guugagcacc     1140

ucugacuuua cauacuuccu cgucgaagau auccuguuuu aucaaagacc uuuaaagucc     1200

cagaaagggc agaucgacga cugcccauac gagucacacg uguauaggga uaagaaagac     1260

ggaagccugc aacauguucc gcuaaagugu gugaguaaau cucacccccu cuuccaggag     1320

uuccgucugu ggcacuuucu uuccuucuug cggauuuacc agcgagaaag aaugauagau     1380

gguagccuga agcucgacgu cgaugugacu aacgaguuuc ugcagaacga agacgauuau     1440

guaaaacuuu ucgacuggcu gaaugagagg aaggaaaucg aucaaaagac cauuuuggcc     1500

uacaaaccuu uuggccucaa gaaaaacgag cagucgaagu aucgcuggaa uuacguugaa     1560

gacaaaucuu acccaugcaa cuccacacgg gcagauauga ucuucagacu gucaaaggcu     1620

ggggugcccg aggaguuuuu aaccagggaa aaggaggaag cccuguggca uauacuauau     1680

aguauuagcg acaaaaagga gcucacgaaa gcgcugggaa cuuucgccga aaagaauggc     1740

cuuaacgaga cauuugucga gaucuucucc aaaaugccuc cauuugaauc ugauuacgca     1800

gcuuauagcu ugaaggccau ccgcaagcug cucgcucuga ugcgaauggg uaaauacugg     1860

gacgagcagg ccauugacga acacacccgg ucacguaucg agaagauuuu aacuggggaa     1920

uaugaugaga caauaagaac cagggugcgc gagaaagcaa ugcaucugac ggacaucagu     1980

uccuuccggg gccuucccuu guggcucgcc uguuacgucg uguaugauag acacagcgaa     2040

gcuaaggagc ugguuaaaug ggaaaccccg gacgauauug accauuuucu aaggaaguuc     2100

aagcagcacu cucugcgaaa uccuaucgug gagcaaauca uuauggagac acuccgcacu     2160

guacgggaua uauggaaaca ggaaggaaag aucgacgaga uucacgucga acugggcaga     2220

gagaugaaaa accccgcgaa ggaaagggca cguaucaccg cccaggugcu ugagaaugag     2280

aacacaaauu ugcgcauuaa agcucuguua gccgaguuca ugaacccaga auuugaaauc     2340

gagaauguuc ggccuuacuc acccgcacag caagaaauac ucagaaucua cgaggagggg     2400

gugcugagug gagucgccga aaaggaucuu ccagacgaua uugcuagcau ccugaagaaa     2460

uucagggaga acgacgugaa gaaacgaccc acuaccuccg aaguauugcg guauaagcuc     2520

uggcuggagc agcgcuacag aucuccuuau acagguagga uuaucccacu aggcaaacug     2580

uuuaccccgg ccuacgaaau agagcaugug auuccccagu cgcgguauuu cgaugacagc     2640

uuuacuaaua agguuaucug cgaguccgca gucaacaaac ucaaggacaa cugucugggg     2700

uacgaguucg ugaagaaaca cucaggagaa aagguggaac uuggcaaugg ugagacggcg     2760

acaauuuuua gugucgagga auaugagcga uucguuaaag auucuuacuu uaacaauagc     2820

aagaaaauca agaaguugcu guuagaagac auaccugaua acuucaucga gagacagcuc     2880

aaugacuccc gcuacauuuc aaaaguggua aagagccugc uuucuaacau cguaugcgaa     2940

gagggggagc aagaaggcau guccaaaaau gucauugugu guaccggagg cguuacugau     3000

aggcugaaga aagacugggg ugugcaggau gucuggaacc guaucauacu accacgguuu     3060

cucagacuga auaagaugac cgacaggaca gauuucaccg cugugaguac uaacggacau     3120

uugcugcccg ccguuccuuu acaccuccag aaggguuuua auaaaaagcg cauugaccac     3180

cgacaucacg caauggaugc caucgugauc gcuugcgccg gcagaaacau uguaaauuau     3240

cugaacaaug caagcgcucg gaaagggucu gagaucucca gguacgaccu ucaacaucug     3300

uugugugaca aggucaaaac agaugccaac ggaaacuaua aguggauucu ccgcaagcca     3360

ugggaaacgu ucacccagga ccuguacgcg gcccuaacua auauaguggu gucauucaaa     3420

cagaaccugc gugucaucaa uagaacaacc aaccacuauc agcauuacaa ugaggauggc     3480

gaaaagaaaa ugauucccca aacaaagggu gaccggcuug cuaucaggaa accgaugcac     3540

aaggauacug uuuaugggga ggugaaccuc cgcaaggaga aaacccugcc uuugaaggac     3600

guagugaaaa auccaagugc agucguggau aagagccuga aaaacaaguu auacgaacuc     3660

cugaaaucgc aguacgaccu uaaggccauu acgaaguauu uugagaccca ccaggauguu     3720

ugggcugacg ugaaucugaa aaagaucaaa gucuacuauu ucacaaagga aacuaacgag     3780

cgauuuuucg ccaccagaaa auccuuggau ucuagcuuuu cacagaagaa gauagaagag     3840

gaggugacag acacuggcau ccaaaagauu cuccugcggc aucuacagca gaauggaaac     3900

aaucccgaua ucgcauucag uccugacggc guugaaagga ugaaccagaa uaugaccauu     3960

cugaacgacg ggaaguggca ccaacccauc uacaaagugc gcacguauga gaaggcggau     4020

aaauuugccg ucggagaaaa gggcaacaag gugaaaaagu ucguggaggc ugccaaaggu     4080

acaaaucucu uuuucgcagu uuacgagucc auacaggaag acgaggcuac cgggaaacag     4140

gugagaaaga ggacuuuugc cacaauucca cuuaacgaag ucaucaagcg gaagaaacaa     4200

ggacugccug cagcccccga ggauucuaag ggcaauuugc caaucuucgu guuaagcccg     4260

aacgaccugg uauaucuccc uaccgaagag gagcguaauu ccgguaaaau uauacagccc     4320

cuggaucgcg aacgaaucua caagauggug ucaugcacug agggcgaaug ccauuguauu     4380

ccagaguacg ucgcuaaccc cauccuucag accauugaac uggggagcaa uaacaaagcg     4440

cagagagccu ggaguggaga gaugaucaag gagauaugcg uuccugugaa aguggacagg     4500

uugggcaaug ucucucuaau caagauuuga                                      4530


<210>  3427
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3427
augaagacca uccugggccu cgaccuuggg acaaacagcg ugggaugggc cgucauuaau       60

uccguuauaa aagagcaggc ugaaagaauc uggauugaga cugcagguuc uaggaucauu      120

cccauggaug ccgcggugcu gggcgacuuc gauaagggga acucaaucag ucaaacgagc      180

gaacggaccc gcuugcgagg agcucguaga uuaagggagc ggcagcugcu acgcagagaa      240

aggcuccaca aaguacugaa uauacuuggc uuucugccua accauuacuu ggaguauauc      300

gacuucgaga agcacccagg uaaauuucuc gccgaugcag aaccgaagcu ggccugggug      360

aaggacgagc aggggaaaua cacauuccug uuucaggcug cauucgauga aaugcucgcc      420

gacuuugcua uucaucaacc cuccuuaguc ucuggagaua agaaaauccc uuaugacugg      480

acuauuuacu aucugcggaa gaaagcccuu accgagauga ucucgaagga ggaauuggcg      540

uggauacugc uaaauuucaa ccagaagcgc ggcuacuauc agcuccgagg cgaggaagag      600

gaagauaaug cagggaaauc aguggaguuu uacgcccuga agguucuuac aguggaggcu      660

accgacgaaa agaagggaag agaugucugg uacaacgugc accuggagaa ugguuggauu      720

uauaggcgga gcaguuccgu accauuggac ugggaaggcc agguuaaaga guucaucgug      780

acuacagacc ucaaccccga ugggaccccu aagcuggaca aauacggaga agucaagcgu      840

ucuuuucgcg ccccaaagga ggaugacugg auguuaauua aaaagagaac ggaggcagau      900

aucgcuaaua cucauaaaac cgugggcgcc uauaucuacg acacacugcu ccaaaagccc      960

cagcagaaaa uuauagguaa acuugugaag acugucgaaa ggaaacugua ucgggaugag     1020

uugagccuaa uccugcagaa gcaaugcgcg uuccacgccg aacuccagga ccgagcacug     1080

uacgcucgcu guguugaggc ccuuuauccu uccaacgaag ugagaaggaa aaauauugcu     1140

aaccgggauu uuguauaccu guugauggac gauauccucu ucuaccagcg cccacugaag     1200

accaaaaagu cauuaauagc caauugcccg uaugaggaga acgaauacgu ggacagagag     1260

acaggggaaa uuaagaaagc accccugaag uguaucgcca aaagucaccc ucuauuccaa     1320

gaguuuaggc ucuggaacuu ccugagcaau auccguauuu aucagaagga gaaagaaauc     1380

aacggaaccc uucauuugga cgucgaugug acggcugagu uucugaagac ugaagacgau     1440

uacguugcuc ucuucgacug gcugaauagc cggaaggaga uugaacagaa aacauuucuu     1500

aaguauccaa aauucggccu gaagaaagag auauccaacu accgauggaa uuauguggag     1560

gauaagcagu accccugcaa cgaaaccaga cacgcaaucu ugucaagccu cgagaaagcc     1620

aaugucugua uugacuuucu gaguaacgaa aaggaggugg cuuuauggca uauccuguac     1680

ucuguauccg auaagcaaga aaucgagaaa gcccuaagga cuuucgcagc caagaauggc     1740

cucgacgaga gcuuuguuga agcguucaaa aaguuuccuc cauucaaauc ggaguauggg     1800

gccuacucug caaaggcgau uaagaaaaug cugccccuua ugcgcugcgg aaaguauugg     1860

gauguguccu caauagacga uacaaccaaa gaacggauca agaaaauugu cacgggugag     1920

uacgacgaaa gaaucgcugu gaaggucagg gauaagguga ugaacuugag ugacauuagc     1980

uguuuucgcc gacugccgcu cuggcuggcc ugcuauaucg uuuacaaucg gcacuccgag     2040

gacaaagagg cagugaagug ggaaucuccu gccgauauag acgcuuauuu agccagcuuc     2100

aaacagcacu cacugcguaa ccccaucgua gagcagguca uuaccgaaac acuuagagug     2160

guuagggaua ucuggaaaca ggugggccgc auugacgaga uccauauaga guuggggcgg     2220

gaaaugaaaa auccagcaga uaagagaaag aggaugacug cucaaauucu cgagaacgaa     2280

aaugccaacc ugcgaaucaa agcgcuacug gccgaguuca ugaacccuga auacgagauu     2340

gagaaugucc gccccuacag uccaucccag caggaaaucc uccggauaua ugaggacacc     2400

gugcugcacg cugaagauca gcuucccgag gaaaucgacg caauuuugaa gaaauuuaac     2460

agcucuaagg uaccuacaaa aucagaguuc cugagauaca aguuauggcu cgagcaaaaa     2520

uauaggaguc cauacacugg agaacugauc ccgcuuggca agcuguuuac cgccgcuuau     2580

gagauugaac augugauccc ccagagccgu uacuucgaug acuccuuuuc uaauaagguu     2640

auaugugagu cggccgucaa caaauugaag gauaaucagc ucgguuauga guucauuaaa     2700

aaccaccaug ggcagaaggu ggaagugggc uuuggaaaaa gcgucgagau cuucuccguu     2760

gacucauacg aacgcuuugu gaaggagcaa uacgcaaaga gugcggugaa aaugaagaaa     2820

cugcuaaugg augacauccc ugaacaguuc auugagcggc agcugaauga cucuagauau     2880

aucagcaagg uaauuaaagg ccuccugucc aacauaguca gggagaagaa ugauucaggg     2940

gaauacgagc cagaagccgu gagcaagaac aucguugugu gcacaggauc ugucaccgac     3000

cgacuuaaac gggauugggg uaugaaugac guguggaacu ccauuguuua uccccgcuuu     3060

gagagauuga auagucugac ugauacgcaa ucuuucggcc acugggaaaa caagucuggg     3120

aaaaaggugu uucagacaga guuaccucuc gaguaccaga aaggauucuc caagaagagg     3180

aucgaccacc ggcaucacgc uauggaugcc auuguaaucg caugugcuac ccgaaaucau     3240

gucaacuauc ugucaaacga aagugccagc agaaaugcaa aaauaucgcg cuacgaccuu     3300

cagaggcugc uaugcgauaa gaaccguacu gacgauaaug gcaacuauaa augggugauc     3360

aagaaaccau gggacaccuu cacacaagac gcccgggagg ugcucuccaa gauugucguu     3420

ucucugaagc agaauuugag aaucauuaac aaaaccacua auguguacag gcacuuugau     3480

gcuacaguaa accgcgugua cgaaaagcag aaaacgggug acagcugggc gauccgaaag     3540

cccaugcaca aagauaccgu cuucggggcc gugaaucuga gaaagguuaa agugguccgg     3600

uuaucagugg cacucgacac uccggcuaug auaguugaua agagggugaa gaacaaaguc     3660

uuugagcugc uuaguuauaa guacgacaaa aagcgcauug aaaaauauuu caaggagaau     3720

gccuuucugu ggaaggaguu ggaucucgcc aaaguagcug uguacuauuu cacagaagca     3780

accacagagc cucugguugc cgugcguaag ccacuagacu ccacuuuuaa cgaaaagaag     3840

aucaaagagu cugucaccga uacgggcauc cagaagauuc ugcuuaauca ucucugcacc     3900

aaggaaggaa aacccgaccu ggcguucagc gcugagggca uagaggaaau gaacagaaau     3960

uugcugcaau uaaacaacgg gaagucccac cagccuaucu acaaagugcg gguauaugag     4020

cccaggggaa auaaguuuuc aguggguguc cucggcaaca aagggacaaa guggguugaa     4080

gccgcaaagg gaacuaaucu guucuuugcc auuuacauca ccgaggaugg caaacgcaca     4140

uacgagacua uuccacuuaa cguggugauc gaacgagaga agcagggucu ggucccugug     4200

cccgacagaa augaaaaagg ggauaagcuc uuguucuggc ugagcccaaa cgaccuaguu     4260

uaucugccga ccgaggagga acgggaguuc ggcaggauaa augaaccuau cgaccgcgga     4320

agaauuuaca aaaugguaag uuguacgggc aacgaggggc auuuuauccc cgcuaaugug     4380

gcaaacccaa uuguccccgu gaucgaacuu ggaucuaaua acaaggccca gagggcgugg     4440

acaaaugaga ugauaaaaga uauuugcauc ccuaucaagg uugaccggcu cggccacauu     4500

aucgaaguga aguaucguau agaugaguga                                      4530


<210>  3428
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3428
augaagaaaa cccugggccu cgaccuuggg acaaacagca ucggaugggc cgugauuaau       60

uccaacauag auucugaggg uaaggaaaaa cuggucggca ucucaaguug cgggagcaga      120

auuaucccca uggacgcuac uacguuggga gauuucggca aggguaauac caaauccccu      180

guugcagaga ggacacgguu acgcgggauu cgacgucugc uagaaagauc ucuccugagg      240

cgggagcgcc uucacagagu gcugucggua augggauuuu ugccagaaca uuacgccuca      300

cagcucgaca gguauggcaa guuccugccg gagacugagc ccaagcuggc gugguacaaa      360

gaugacagcg gccgguauca auuucucuuc cagaagaguu uucacgaaau guuagaggau      420

uuccgccagc aucagccuga acugguggcu ggggagaaaa agaucccaua cgacuggacc      480

auauauuacc uucgaaagaa ggccuugucc caagagauca caaaggaaga acuggcaugg      540

auucuacuca acuuuaauca gaaaagagga uauuaccagc ugagggguga agaggaacag      600

gaggagaaca auaagucugu cgaauaccac gcccuuaaag ugguuagcgu ggaggauucc      660

ggcgaacgga aggggaaaga caucugguau aacgucaccc uggagaaugg auggguguac      720

cgucgcgcuu caaacauucc cuuggauugg acuggcaagg uaaaagaauu uguugugaca      780

accgagcucg acgaugcagg uaauccuaag aaggacaaag aggggaacgu caagagaagu      840

uuuagggccc caaaagaaga cgauugggga cuguuaaaga cgcggacuca agcucagauc      900

gacgagagcg gcaaaaccgu gggcacauau auauacgaau cucugcucug uaugcccaau      960

cagaagauuc gagggaaacu ugugcgcacu aucgagagaa aauauuacaa ggaugaacug     1020

aggcagauuu uggucaaaca auccgaguuc caugccgcgc uacaggacca caaccugcuc     1080

cugucaugca ucgaggaacu uuauccuaau aacgaggcac accggcgccu guugagcgcc     1140

aguucuuuua ucuacuuccu cauugaagau auacuguuuu accagagacc auuaaagucc     1200

caaaaaggac ugaucgacaa cuguccguau gagagccaua uuuacaagga uaagaaagac     1260

gguucgcuac accauguucc ccucaagugc gugucuaaau cccacccucu guuccaggag     1320

uucaggcuuu ggcaguuuuu gucaaaucug cguaucuauc agcgggaacg aaugauggau     1380

ggcagucuca agcuggacgu agaugugacc agagaguucc uuccaagcga agaggacuac     1440

gucaaacugu uugacugguu gaacgaaagg aaggagauau cccaaaaguu ccuccuggcu     1500

uauaaacccu uuggguuaaa gaaaaaugag caggccaacu accgcuggaa uuauguggaa     1560

gauaagucuu acccuuguaa cgagacacgg gcagaaauua aaagcagacu gucaaaggcu     1620

ggaguuccag aggaauuucu aaccgaggag aaagaagagg cccucuggca cauccuguac     1680

aguaucuccg acaagaagga acuuacgaaa gcguugggca cuuucgccgc aaagaauugc     1740

cugaacgaga gcuucgugga agucuuugcu aaaauucccc cguucgaguc uaauuaugcc     1800

gcuuacucac ucaaggccau caggaaacug uuagcacuga ugcgcauggg uaaguauugg     1860

aacgagcagg ccauugaucg acagacacgg gaccguauag aaaagauccu uaccggggag     1920

uacgaugaaa cuauuagaag uagggugcgc gagaaagcua uguugcucac agacaucagc     1980

uccuuucggg gccugccucu auggcuggcg uguuauaucg uauacgauag acauucugag     2040

ucgaaggaac ucguuaaaug ggagaccccc gcagacauug aucacuuccu gagcaaguuu     2100

aaacaaaauu cccuuaggaa cccaauagug gaacagguca ucacggaguc auugcgaacc     2160

gugcgcgaca uuuggaagca ggaaggaaag aucgaugaga uucaugucga gcugggccgg     2220

gaaaugaaaa auccugccaa ggagagagcu aggaucacag cccaggugca agaaaacgag     2280

aauacuaacu uacguauaaa agcacuccug gccgaguuca ugaaccccga guucgagauc     2340

gaaaauguuc acccauauag ucccgggcag caggagauuc uucgcaucua cgaagacgga     2400

gugcugucug guauugcuga gaaggauuug ccugacgaca ucaccgccau acucaaaaag     2460

uuucgggaaa acgauguaaa gaaaagacca acaacuagcg agguccugag guacaagcua     2520

uggcuggagc agcgauaucg guccccguac accggccgcg ugauuccccu cgggaaacug     2580

uucacgccug cauaugaaau cgagcauguu auuccacaau caagauacuu ugacgauagc     2640

aucucuaaua aggugauaug cgaauccgcg gucaacaaac uuaaggacaa uuguuuggga     2700

uaugaguuca ucaaaaagca caguggcgag augguggaac uggguaacgg ggagaccgua     2760

cccguguuua gcguugaaga guacgaaagg uucgucaagg agucuuauuu uggcaauucc     2820

aaaaagauga aaaaguuacu ccuggaggau auuccugacu cauucaucga acggcagcuu     2880

aacgauaguc gauacauuag cagaguggug acuucgcugc uauccaaucu cgucugcgag     2940

gaaggagagc aggacggccu gucuaaaaac guuaucgugu guacaggggg aauaacagau     3000

cgcuugaaga aggacugggg ugugcaagaa guauggaaua ggauuauccu gccacguuuc     3060

uuacggcuca acgagaucac cggcagaacu gauuucacca gcacaucagu caaugggcau     3120

cugcuucccg cucugccguu guaccuccag aaaggauuua acaagaaaag gauugaccac     3180

cgccaccaug ccauggaugc aaucgugauu gccugcgcua aucgaaacau aguuaacuau     3240

cugaauaaca guuccgccag aaagaacucu gagaucagcc gguacgaccu acagaggcug     3300

cuuugcgaaa aagugaagac cgacgcaaau ggcaauuaua aguggauucu ccgcaaaccu     3360

ugggagacuu ucacacagga ugucuacgcu gcccugacga acaucguggu uuccuuuaag     3420

caaaauuugc gugugauuaa cagaaccacu aauuauuacc agcacuauaa cgaagcgggu     3480

gagaaacgga ugaucccaca gacaaagggg gacagguggg ccauacgcaa acccaugcac     3540

aaggauaccg uauacggcga agucaaucug cgaaaggaga aaacauuacc ucucagagac     3600

guggugaaga accccucaau cgucguugau aaaagccuga agaauaaacu uuacgagcug     3660

uugaagaguc aguaugaccu caaggcuauu gcaaaauacu ucgaaacuca ucaagaugug     3720

ugggccgacg uaaaccugaa gaaaaucaag guguauuacu ucaccaaaga gacgaaugaa     3780

cgguuuuucg cuaccaggaa gucucuagau ccauccuuug accagaaaaa gauugaggag     3840

gaagucacag auacuggagu gcagaagauc cugcuucacc aucuccagca aaacaacaau     3900

gacccugaca uggccuucag ccccgauggc auagaccgca ugaaccagaa uaugaccauu     3960

cugaacgaug ggaaauggca ccagccaauc uauaagguua gaacauacga gaaagcagac     4020

aaguuugcgg ugggagaauc gggcaauaaa gccaagaagu ucgucgaggc ugccaaaggu     4080

acuaacuugu uuuucgcagu guaugaauca guucaagagg augaggcuau cgggaaacag     4140

guguguaaga ggaccuuugc cacgauuccg uuaaaugaag ucauaaagcg gaaaaagcag     4200

ggacugccug cagcccccga ggaccucaac ggcaaucugc caaaguucgu acuuucuccc     4260

aacgaucugg uguacuugcc uacagaagag gaacguaaua guucccgcau cauucagcca     4320

cuagaccgag agagaaucua caaaaugguu agcucuagcg guucccaaug cuuuuucauu     4380

aagguguucg ucgcuaacuc aaucugggau aaaaaugagu auaguagccu gaacaagaug     4440

gaaagggcga uaaccaauga gaugaucaaa gaaauuugug ugcccaucaa gauugaccgg     4500

cucggcaacg uaucucugau ccagauauga                                      4530


<210>  3429
<211>  4533
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3429
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugguggau       60

uccgaggaac agaggauauu ggguaugggc ucucggauca uucccaugga ccaagggguc      120

uuagauacau ucucaggagg caauccuguu gagacucaga cggcugcacg caccguguac      180

cgagguacac guagacugag ggaacgggcc cuacuccgca gagagaggcu gauccgggua      240

cuuaacauga ugaaauuucu gccagaacac uaugcgaguc agauugacuu cgagaagcgc      300

uuggggcagu uuuucgauga gaaagaaccg aagcucgcuu ggaaacaaaa ugacgaggga      360

cgauuugaau uuaucuuuca gcagagcuuc aacgagauga uagaggaauu uaagaauaag      420

ggcgugguca gagaggauaa aaagaucccc uacgacugga cuauuuauua ccugaggaaa      480

aaggcccugu cccaggaaau cgagaaagaa gaacucgcau ggauuuuacu gaacuucaau      540

caaaagcggg gcuauuacca gcuucguggg gaggaagagg aagagaaccc uaauaaguug      600

guggaauauu acucucugaa aaucguugau gugauagccg acgagaagcc aaacucgaaa      660

ucagauauuu gguacagccu acaucucgag aauggaugga ucuaucgcag aaguuccaag      720

auuccccugu uugguuggaa agacaagacc agggauuuca ucgucacaac cgaccuuaac      780

gacgauggca cugugaaaaa ggacaaggaa gggaucgaga aacggucuuu ucgagcuccu      840

agcgaaaaug auuggguacu guugaagaaa aagacagagu ccgacauuga aaaaucacac      900

aagaccguug gagcauacgu guaugagacg cuccugcaga acccaaugca gaagguccgc      960

ggcaaauuag ugagaacuau agagaggaag uucuacaaag augaacugaa gcaaauccuc     1020

gagaaacagc gggaguucca ucccgagcuu cgcgacgagg aucuguauaa ugacugcgug     1080

agagaauugu acaggaguaa cgagaccuau caguuuauuc uaagcaagcg ugauuucguc     1140

caccuguuuc ucgacgauau ccuguucuac caacggccuc uucgaucuca gaaguccgcc     1200

auaggugacu guauucugga auuuaaaaca uacaaggaca aagaugggaa caauaucaag     1260

gaguauuuga aaguuaucuc aaagagcaac ccacucuacc aggaguucag aauuuggcag     1320

uggcuguaua auuuaaagau cuacaggaaa gaggacgaug aggacgugac ucugcaauuu     1380

auugcuaaca uagaagauaa ggagcagcua uucgacuuuc ucaguaaucg caaaucuauc     1440

gaacagaagc cgcugcuuga guauuugauu aaagccaagg gacugaaaaa gcagguaaag     1500

accgaagcgu accgguggaa cuauguggag gauaaaaucu accccugcuc cgagacaaag     1560

accaugauca gcacgagacu cgcaaaaguc gaaaauauuc cugacaacuu cuuuacuaag     1620

gagauagaac aaaaacugug gcauaucauu uacucgguga cagauaagaa ugaguucgaa     1680

aaggcccuua ggaccuuugc uaaaaaguau gaccuggaua ucgccucuuu cguugacaac     1740

uucaaaaaga uuccacccuu uaaaaaugag uacggcuccu ucucagagaa ggcaaucaag     1800

aaauugcucc cucugaugcg cuuaggcaag uauuggaaau ggaacgauau agacaauaac     1860

acucagaauc gaaucaguaa gauuaucaca ggggaauacg acgaggaaau uaaagauauc     1920

gugcgggaaa agagcauagc ucugaccaac gagaacgacu uucagggacu acagcucugg     1980

cuggcccaau aucuugucua cggucgucac uccgaagcgg auauugccgg caaguggcac     2040

ucuguggcag acuuggagaa auaucugaau gaguucaagc agcauagccu cagaaaccca     2100

aucguagaac agauuaucac ggaaacccug aggguugugc gcgauauaug gcggaaauac     2160

gggcagggag cugagaauuu cuucucagag auccacgucg aauuaggcag agacaugaag     2220

aacacagccg augagaggaa aaagauugug aaugucguga cugaaaacga gaauaccaac     2280

cugcgaauca aagcucuuuu gauggaacuc aagaauaaca gugacgguaa gcuggagguu     2340

gagaaugugc gccccuacuc cccgacacaa caggauauuc uaaaaaucua ugaagaguac     2400

gccauaagca cugggcugga caacgaaaag gaugagaaag uaaaggaaga cauuaaaaag     2460

aucucucggg ucgcacagcc uaccacaacc gagcuccaaa gauauaagcu guggcuugag     2520

cagaaauacu guucacccua uacuggcaag gugaucccau ugggaaaacu guuuacggaa     2580

gaguaccaga uugaacauau cauuccuaag aguagguauu acgaugacag cuucuccaau     2640

aaaguuauau gcgaggccgc ugugaacaag uuaaaggaca aaugucucgg ccuggaguuu     2700

aucaagaauu accacgggca gauugucgaa acaggauucg gucaaaaagu gaccaucuuu     2760

gaugaggaag cguaucagaa cuucguaaag cagcacuacg cauauaacca uucuaaacgu     2820

acuaaacuuc uguuggagga aauucccgag aagaugaucg agcgccagau gaaugacacc     2880

cgguacauau cgaaguuugu gcucccacug cuaagcaacc ugguuagagc caaggaaaau     2940

gauaacggcg ucaauuccaa aaacgugcuc cccgugaaug ggaagaucac aaccaugcug     3000

aaacaagacu ggggacuuaa cgaugucugg aaugacuuga uucugccuag guucguucga     3060

augaacgagu uagcuaagac uaucgccuuc acaucaugga augaacagca ccagaaguau     3120

cucccaacgg ugccgcugga gcuuaguaaa ggcuuucaga agaaacggau ugaucaucgc     3180

caccaugcaa uggacgcccu ggugaucgcu ugcgccacca gagaucacgu aaaccuacuc     3240

aauaacaagc acgcaaauac ugacacaaua agguacgauc ugcaacggaa auugcgacug     3300

uucgaaagag ucaccuauau ugacccccag acaaagaaca acgugacuaa agacaucccu     3360

aaggaguuua agaaaccaug ggauaauuuc accguugacg cgcgcaacga guuagaaaag     3420

aucauugugu cuuuuaaaca gaaucucagg auaaucaaca aggcuacgaa uauuuacacc     3480

aaauacgaga acgguaagaa gaucaaaguc gggcaaaagg gccugaauug ggccauucgu     3540

aaaccccuuc auaaggaaac aguguucgca aaaguuagcc ugcggaagag aaagacugug     3600

agguuguccg aggcccucaa agauuggaag aaaaucguag acaagaaacu gaagcaggaa     3660

auaaagcgcc uaaccuguca guauggaaaa uuugaugucg acacaauccu gcgauacuuc     3720

aaggauagaa aauaucaguu uggcgaggug gacgugucaa aggucaaaau guacuauuuc     3780

gaugaggaaa acgcugccgu ucggaagaau guggacacua gcuuuaccga gaaauucauu     3840

caagggucug uaacggauac aggaauccag aagauucuuc ucaaccaccu ggaagcaaag     3900

gguaauaaag uggagaucgc uuucuccccu gagggcauag aagagaugaa caagaauauu     3960

auccaguuga acgaagggaa acugcauuua ccaaucuuua aggucagggu guacgagacc     4020

auuggaaaua aauucagugu uggcgugaag gguaacaaga aagacaagua ugucgaagcc     4080

gcgaaaggga cuaaucucuu uuucgccauc uacguggaug agaacggcau acgcagcuac     4140

gagacaauuc cccugaacau cguuauugaa cgucuuaagc agggacuguc uguggucccg     4200

gagaaaaaua gaaaggaaca cuccuugcuc uuuuaucugu caccuaacga ccuaguauac     4260

gugccaaucg aggacgaacg ggagaauaua cacgcuguua accuggauca acuuaauaac     4320

gagcagagga agcgcauuua uaaaauggug aguugcaccg gcagcgaaug ucauuucguc     4380

cccuacuaug uggcaucgcc uaucguaaau aagguggagu acuccucucu caacaaaauc     4440

gggcgaagcc ugacuggaga aaugauuaag gacgucugca ucaaauugaa gguugauaga     4500

uuaggcaaua uuaaggguau aucagggcug uga                                  4533


<210>  3430
<211>  4533
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3430
augaagagaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugguggau       60

uccgaggaac agaggauauu ggguaugggc ucucggauca uucccaugga ccaagggguc      120

uuagauacau ucucaggagg caauccuguu gagacucaga cggcugcacg caccgccuac      180

cgagguacac guagacugag ggaacgggcg cuacuccgca gagagaggcu gauccgggug      240

cuuaacauga ugaaauuucu gccagaacac uaugcuaguc agauugacuu cgagaagcgc      300

uuggggcagu uuuucgauga gaaagaaccg aagcucgccu ggaaacaaaa ugacgaggga      360

cgauuugaau uuaucuuuca gcagagcuuc aacgagauga uagaggaauu uaagaauaag      420

ggcguaguga gagaggauaa aaagaucccc uacgacugga cuauuuauua ccugaggaaa      480

aaggcacugu cccaggaaau cgagaaagaa gaacucgccu ggauuuuacu gaacuucaau      540

caaaagcggg gcuauuacca gcuucguggg gaggaagagg aagagaaccc uaauaaguug      600

gucgaauauu acucucugaa aaucguggau guuauagcug acgagaagcc aaacucgaaa      660

ggagauauuu gguacucacu acaucucgag aaugguugga ucuaucgcag aagcaguaag      720

auuccccugu uuggcuggaa agacaagacc agggauuuca ucgugacaac cgaccuuaac      780

gacgauggga cugucaaaaa ggacaaggaa ggaaucgaga aacgguccuu ucgagcaccu      840

ucugaaaaug auugggugcu guugaagaaa aagacagaga gcgacauuga aaaaucccac      900

aagaccguag gcgccuacgu uuaugagacg cuccugcaga acccaaugca gaaggugcgc      960

gguaaauuag ucagaacuau agagaggaag uucuacaaag augaacugaa gcaaauccuc     1020

gagaaacagc gggaguucca ucccgagcuu cgcgacgagg aucuguauaa ugacugcgug     1080

agagaauugu acaggucaaa cgagaccuau caguuuauuc uaaguaagcg ugauuucgug     1140

caccuguuuc ucgacgauau ccuguucuac caacggccuc uucgaagcca gaagucugcu     1200

auaggggacu guauucugga auuuaaaaca uacaaggaca aagauggaaa caauaucaag     1260

gaguauuuga aagucaucuc caagucaaac ccacucuacc aggaguucag aauuuggcag     1320

uggcuguaua auuuaaagau cuacaggaaa gaagacgaug aggacguuac ucugcaauuu     1380

auugccaaca uagaagauaa ggagcagcua uucgacuuuc ucagcaaucg caaaaguauc     1440

gaacagaagc cgcugcuuga guauuugauu aaagcgaagg gccugaaaaa gcaagugaag     1500

accgaagcau accgguggaa cuauguagag gauaaaaucu accccugcuc ugagacaaag     1560

accaugaucu ccacgagacu cgccaaagug gaaaauauuc cugacaacuu cuuuacuaag     1620

gagauagaac agaaacugug gcauaucauu uacagcguca cagauaagaa ugaguucgaa     1680

aaggcucuua ggaccuuugc caaaaaguau gaccuggaua ucgcaucguu cguggacaac     1740

uucaaaaaga uuccacccuu uaaaaaugag uacggcucuu ucuccgagaa ggcuaucaag     1800

aaauugcucc cucugaugcg cuuagggaag uauuggaaau ggaacgauau agacaauaac     1860

acucagaauc gaaucucaaa gauuaucaca ggagaauacg acgaggaaau uaaagauauc     1920

guucgggaga agaguauagc ccugaccaac gagaacgacu uucagggucu acagcucugg     1980

cuggcgcaau aucuugugua cggccgucac agcgaagccg auauugcagg gaaguggcac     2040

uccgucgcug acuuggagaa auaucugaau gaguucaagc agcauucucu cagaaaccca     2100

aucguggaac agauuaucac ggaaacccug aggguaguuc gcgauauaug gcggaaauac     2160

ggacagggcg ccgagaauuu cuucagcgag auccacgugg aauuagguag agacaugaag     2220

aacacagcug augagaggaa aaagauuguc aaugugguca cugaaaacga gaauaccaac     2280

cugcgaauca aagcccuuuu gauggaacuc aagaauaacu cagacgggaa gcuggaggug     2340

gagaauguuc gccccuacag uccgacacaa caggauauuc uaaaaaucua ugaagaguac     2400

gcaauaucca cuggccugga caacgaaaag gaugagaaag ugaaggaaga cauuaaaaag     2460

aucagccggg uagcccagcc uaccacaacc gagcuccaaa gauauaagcu guggcuugag     2520

cagaaauacu guucucccua uacuggaaag gucaucccau ugggcaaacu guuuacggaa     2580

gaguaccaga uugaacauau cauuccuaag ucaagguauu acgaugacag uuucagcaau     2640

aaagugauau gcgaggcugc gguuaacaag uuaaaggaca aaugucucgg gcuggaguuu     2700

aucaagaauu accacggaca gauuguggaa acagguuucg gccaaaaagu caccaucuuu     2760

gaugaggaag cauaucagaa cuucgugaag cagcacuacg ccuauaaccg uuccaaacgc     2820

acuaaacuuc uguuggagga aauucccgag aagaugaucg agcggcagau gaaugacacc     2880

agauacauau cuaaauuugu acucccacug cuaucgaacc uggugagggc ugaagagaau     2940

gauaacgggg uuaauagcaa gaacguccuc cccgugaaug gaaaaaucac aaccaugcug     3000

aagcaagacu ggggccuuaa cgaugugugg aaugacuuga uucugccucg auucguccgg     3060

augaacgaau uagccaaaac uaucgcauuc acauccugga augagcagca ucagaaguau     3120

cucccaacgg uuccgcugga acuuucaaag gguuuucaga aaaagcgcau ugaucacaga     3180

caucacgcca uggacgcucu ggugaucgcc ugcgcaacca gggaucaugu gaaccuacuc     3240

aauaacaaac acgcgaauac ugacacaaua cgguacgauc ugcaacgaaa guugagacug     3300

uucgagcgcg uaaccuauau ugacccccag acaaagaaca acgucacuaa ggacaucccu     3360

aaagaguuua agaagccaug ggauaauuuc accguggacg cuaggaacga auuagagaaa     3420

aucauuguua guuuuaagca gaaucuccgu auaaucaaca aagccacgaa uauuuacacc     3480

aaguacgaaa acgggaaaaa gaucaaggug ggccaaaaag gacugaauug ggcaauucgg     3540

aagccccuuc acaaagagac agucuucgcc aaggugucuc ugagaaaaag gaagacuguu     3600

cgcuugagcg aagcucucaa ggauuggaaa aagaucgugg acaaaaagcu gaaacaggag     3660

auaaagcgac uaaccuguca guauggcaag uuugauguag acacaauccu gagauacuuc     3720

aaagaucgga aguaucaguu uggggagguc gacgugucca aagugaagau guacuauuuc     3780

gaugaagaga acgccgcagu caggaaaaau guugacacuu cauuuaccga aaaguucauu     3840

caaggaagcg ugacggauac agguauccag aaaauucuuc ucaaccaucu ggaggcuaag     3900

ggcaauaagg uagagaucgc cuucucuccu gaagggauag aggaaaugaa caaaaauauu     3960

auccaguuga acgagggaaa gcugcacuua ccaaucuuua aagugcgcgu cuacgaaacc     4020

auuggcaaua aguucuccgu ggguguuaaa gggaacaaga aggacaaaua uguggaggcg     4080

gccaagggca cuaaucucuu uuucgcuauc uacgucgaug agaacggaau acguaguuac     4140

gaaacaauuc cccugaacau cgugauugag agacuuaaac agggccugag cguugugccg     4200

gaaaagaauc ggaaagagca uucuuugcuc uuuuaucugu ccccuaacga ccuagucuac     4260

guaccaaucg aagacgagag ggagaauaua cacgcaguga accuggauca acuuaauaac     4320

gaacagcgca agcgaauuua uaagaugguu ucaugcaccg ggagugagug ucacuucgug     4380

cccuacuaug ucgccagccc uaucgugaau aaaguagaau acucgucccu caacaagauc     4440

ggaagaucuc ugacuggcga gaugauuaaa gacgugugca ucaaguugaa agucgaucgg     4500

uuagguaaug uuaaggggau uagcggacug uga                                  4533


<210>  3431
<211>  4536
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3431
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cgugauaaau       60

uccgucgcug augagucugu ugaaaagaga aacaucgagc uggcagguuc aaggauuauc      120

cccauggacg ccgcgguguu gggcaauuuc gauaacgggg uaacaaaaag ucagacugcu      180

gaacggacga gcuaccgcuc cauucgacgu uuaagagaga ggcaacugcu acggcgcgaa      240

agacuccaca aggugcugca gauccuugga uuucugccug agcauuaugu caaugacaua      300

gauuucgaga accacccagg caaaaucaag aagaauaccg aaccgaaauu gcccuggagg      360

aagaacucua ugggucaguu ugaguucauu uuucaggccu cguucgaagc aaugcucaca      420

gacuuugcca cucaucaacc uguguucguu ucagagggga aaaaggugca cuacgauugg      480

acccuguauu accugcggaa aaaggcucuc acagaggaaa ucagcaagga ggaauuagca      540

uggauuaucc ugaauuuuaa ccagaaacgc ggauauuacc agcuucgagg cgaggaagag      600

gaggaacaga guaauaaguu ggucgaguuc uaugcccuga gagugguaaa aguugaagcu      660

accgacgaua agaaaggcaa ggacacuugg uacaacgugc aucuagagaa uggguggaua      720

uacaggcgga cauccaacau uccacucgau ugggaaggaa aagucaagga guuuaucgug      780

accacggacc ugaaugagga ugguacuccc aaacuugaca aauauggcga agugaagcgu      840

ucuuuccgcg ccccuaaaga ggacgauugg acccugauua agaaaaagac agaaagcgac      900

aucgcgaacu cccacaagac uuuggggugc uacaucuaug augcacuccu gcaaaaccca      960

aaucagaaaa uuaagggaag auuagucagg accguugagc ggaaauuuua caaggaggag     1020

cugcgacuca uacuugagaa acagcuguca uuccaccccg aauugcagga ccgcgaucua     1080

uauagagccu guaucgacgc ucuguacgug aguaacgagg cccauaagaa uaacauuagc     1140

ggcagggauu ucguauaucu cuuuaucgac gauauaauuu ucuaccaacg gccucugaag     1200

acaaagaagu cucuuaucga caauugccca uacgagaaaa accaguauau ggaucgcgaa     1260

accggugagg ugaagcaggc accgaucaaa uguauugcua agucccaccc ccuguuucaa     1320

gaauuuagau uguggcaguu uaucucaaau auuaggauau accagcguga gcgggucaug     1380

gacgacggga agcucgaaac ggauguggac guuacuccug auuuccugaa aacagaggac     1440

gauuauacca cuuuauuuga auggcugaac gaccgaaagg agguggauca gaaagccuuc     1500

cuaaagaauc cacaauuugg acucaaaaag acauucaccg aguacagaug gaacuauguc     1560

gaagacaaaa gcuaccccug caaugagacg aggaacgcga uccugaaugg ccuucagaag     1620

gccggcguga gugcagauuu cuugucucgc gaaguagagg aagcucugug gcauauucuc     1680

uauuccguug aggacaagau cgagaucgaa agcgcccugc ggaaauucgc uucgaagcac     1740

gaucuugacg auagauuugc cgacguguuc aaaaagcugc cuccauucag gaaagaguac     1800

ggggcauacu cugccaaggc uauuaagaaa uugcuccccc ugaugcgcau gggaaaguau     1860

ugggaauuau ccaacauaga cgauaaaacc aaggagcgaa ucgacaaaau uaucacaggu     1920

gaaugggaug agaauauuca caaccggguc cgugagaagg cgaucaagcu gucagacaua     1980

acugauuuua aaggccuacc gcucuggcug aguuguuacg uggucuauaa cagacauagc     2040

gaagcaaggg ccaucgcuaa gugggagucc ccugaagaca uugauucuua ccuucgcuug     2100

uuccggcagc acagccugag aaaucccgug guugaacagg ugaucaccga gacacucagg     2160

acuguacgag acauuuggaa acgcaucggg caaauagaug aaauucaugu cgagcuggga     2220

cgggaaauca agaacccagc cgacaaaaga gcaaagauga ccgcccagau uguggagaau     2280

gaaaacacaa auuuaaggau caaggcucug cuugccgagu uuauaaacuc agaguaugaa     2340

aucgagaaug uucguccuua cagucccggc cagcaggaaa uuuugcgcau cuaugaggau     2400

accgugcuca acuccgucga agagauucca gaggacauca gcgcagugcu gaaaaaguuc     2460

cgggaaucug aucugaaaaa gagacccacu aaaucagagu uucugaggua caagcucugg     2520

cuggaacaaa aauaccgaag uccuuauacg ggugacauaa uuccacuugg gaaguuguuc     2580

acaagcgcgu acgagaucga acacaucauu ccgcaguccc gguauuuuga cgauucuuuc     2640

ucgaauaagg uaaucugcga ggcugaggug aacaaacuaa agagcaauga auuaggcuac     2700

gaguuuauua aaaaccauca cggacaguau guugaaauag gcuucgggaa ggucgugcag     2760

aucuuuucca uugaggccua cgagaaauuc gugaaggcaa auuauucagg aaucgguagu     2820

aagaugaaac gccuccugau ggacgauauu cccgaaaccu uuaucgagag acaacuuaac     2880

gacucuaggu acauaagcaa ggucguuaaa ucccuguugu caaauaucgu gcgggaaaag     2940

gauagcaacg gcgaguacga accugaggcc auuucuaaaa acgugaucgu auguacuggg     3000

uccgucaccg accgacucaa gagagauugg ggagugaaug acguuuggaa caguauuauc     3060

uauccacgcu ucgagaggcu gaaugaacua acaggcagcc agcaguucgg uugcugggag     3120

aacaaggaag ggaaacgugu guuucaaacc gcuguccccc uggagaugca gaagggcuuc     3180

ucuaaaaagc ggauagauca cagacaucac gccauggacg caauugugau cgcuugugcc     3240

aucaggaauc auauuaacua ccucuccaau gaaucagcga guaaaaaggc caagauaagc     3300

cgcaucgauc ugcagcgacu uuugugcacu aaauguagaa cagacgacaa cggaaauuau     3360

aaguggauug uuaaaaagcc uuggccagcu uuuacgcagg auauccggaa agcacuggag     3420

aacauuaccg ugucguucaa gcaaaauuua aggguaauca acaagacuac aaauuacuau     3480

guccacuuug acgaguccgg caaaaaggug cucgaaaaac agauaaacgg ggauucuugg     3540

gccauccgca agcccaugca caaagacacc guguucggaa aggucaaucu gcagaaaguu     3600

aaggaggugc gucuuagccu ggcucuagau acaccgaaga ugauuguaga caaaaagcuc     3660

aaagaaaagg ugcugcaguu guacucauau aaauacgaua agaagagaau cgacaaauac     3720

uuuaaggaga acgccagucu guggaaagau uuaaaccucu ccaaggucgc aguguauuac     3780

uucacuaaug acucuaccga accucugguu gcggugcgga aaccacuuga uacgagcuuu     3840

aacgccaaga agauuaaaga guccgucacc gacacaggua uccaaaagau acuguugaau     3900

caucucucaa acaaucagga uaaccccaau cuggcuuuca gcccugacgg cauugacgag     3960

augaacagga aucuaacuac ccugaacgau gggaaauauc accagcccau cuacaaggug     4020

cgcguuugcg aaccacaggg aaauaaauuu agugugggca caucugguaa caagacuucc     4080

aaguaugucg aggccgcaaa agggaccaau cuuuucuucg cuguauacca aacggaagac     4140

ggcaagcgaa acuaugagac aaucccucuc aaugugguua uugagagaga aaaacuggag     4200

ugggcccccg ugccagaaaa caauaacaac ggagauaagu ugcuguuuug guuaagcccg     4260

aaugaccucg ucuacgugcc uaccgcagau gagcugaaca auggcaugcg gcuugacaac     4320

auggauaaag ggaggaucua caagaugaua ucgugcucag gaccccagug uuucuuuauu     4380

ccacagaaug uaucuaaccc caucauuccu gugaucgaac ugggcgucaa uaacaaagcc     4440

gagaagaguu ggaauaacga gaugauaaag guugugugcg ugccaauucg cgucgacaga     4500

uuggguugua ucacucaaau caauaaccag aaauga                               4536


<210>  3432
<211>  4539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3432
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugauagaa       60

aauuccuuca cagaaaagga ggguaaaauc aacgauaugg gcgugagaau uauccccaug      120

ucugcugaca uuuuggggaa guuugaugca ggacagucac acagucaaac ugccgaaagg      180

acgggcuacc gggguacccg ccgauuauau cagcgugaca aucugagaag ggagcggcua      240

caucgcgucc uccacauccu gggguuccuu ccuaaacauu acgaugaaca cauagacuuu      300

gagaagagac ugggacaguu caaggagggc aaagaaauca aguugaacua uaggugcaau      360

guugagaaca aacaugaauu uauuuucauc agcuccuuua augagaugcu cacagaguuc      420

aagaaagcgc agccagaacu guuuuaccug aagucuaacg gcgaggaaac uaagauuccg      480

uaugauugga cccucuacua uuuacggaaa aaggcucugu cgcaaccccu uacaaaacag      540

gaguuggccu ggaucauacu gaauuucaac cagaagcgcg gguacuacca gcuacgagga      600

gaagagauug acgaugacaa aaauaagcaa uuugugaaac ucaagguaaa ggaggugauc      660

aacucaggug aagcagucaa aggcaagccu cuguucgaug ugauuuuuga gaaugggugg      720

aaauaugaca agcagguugu gaaaaccgaa gauuggaucg acagaacuaa ggaguucauc      780

gucacaacca aggugcuuaa aaacggagac cugaagagga gcuacaaaac gguagauagu      840

gaaaaggacu gggccgcuau uaaagcaaag acugagcagg auauagagcg guccaauaag      900

accguuggcg aguucaucua ugagacauug cucgaaaacc caacucagaa aauucguggu      960

aagcugguga aaaccaucga gcgcaaguuu uacaaagaag aguuaaagaa cauacugauu     1020

aagcaaaucg agcuccagcc cacacuuuuc ucugaagaac uguauaaagc cuguaucgaa     1080

gaguuguacc cuagaaauga ggcucaccag aacgccauua agaauaggga cuuuaucuau     1140

cuacugaugg augacauuau auucuaccaa cggccacuca aaagccagaa guccaacauc     1200

ucagggugcc agcuggaaca gcgagucuac uauaaagcga auaaggauag uggagagaac     1260

gaagagguga aacaagcagu gaaggccauu cccaagagcc acccucuuuu ucaggaguuc     1320

cgcaauuggc aguggcugca gaacuugaaa aucuacaaua aggucgacac cgagaaaggc     1380

gagcucacgg auguuacuaa gcaacuguua ccaucugaag acuccugggu gaaccuguuu     1440

aauuuccuac aguguaaaaa ggagcucgaa cagaagcagu uuaucaaaua uuucauugau     1500

aagaaacuga uaaacaagac agagaaagac cauuacagau ggaauuaugu agaagacaag     1560

aaguacccgu uuaccgagac uagggcucaa uucaucucac ggcuuacaaa aguggagggc     1620

auugaagaga ucaacaccuu uuuggauaag aaaacgcaga uugggaccaa ggacaauagc     1680

cccuucguca gucgcaucga acagcugugg cacauaaucu auucuguguc cgauauuaaa     1740

gaguacgaaa gcgcccucga gaaguucgca aucaagcauc accugaacga ggaaucguuu     1800

guugcuaacu ucauuaaauu uccuccauuc aagucugacu acggauccua uaguaaaaag     1860

gcccuuaaua aacuguugcc ccucaugaga agggguaacu acuggacaga gaaugauauc     1920

ucagaaaagg ugaaacugcg ugucagcgag auaauggagc gggugaacgc guuaaaucug     1980

aaggaagacu auuccucuaa ggagcuagcc gaaacucucg uaaacguuag cgaugacgau     2040

gugaaaaagc agcugauuaa aucauuuguc aguuucaagg accgaaaucc ucuuaugggc     2100

uugaacaccu uucaagcaac auaccuggug uaugggagac acuccgaggu cggagauauc     2160

cagaauugga aaacuccaga cgauauugac accuaccuca agaacuucaa gcagcauagc     2220

cugaggaauc ccaucgugga acaaguugug acagagaccu uacgcguagu ccgggacaua     2280

uggaaacacc auggcaaguc ucagacuaac uuuuucaaug agauccacgu ggaacugggu     2340

agagagauga aaaacccggc ugauaagagg aaacgcauuu cagaacgaaa uacggagaac     2400

gaaaacacaa auaaccggau ccgugagauu cuuaaggacu ugaugaauga ugccaguauc     2460

cagggggacg uuagagauua uagcccuucc cagcaaggca uacucaagau uuacgaggaa     2520

ggaguguacc agaaccccaa agucgacuau ucuaaggugu cggaggauga aaucaccaaa     2580

aucaggcgca gcaauaaccc aacucagaag gagauucagc gguacagacu guggcuagaa     2640

caaggcuaua ucuccccuua uaccugcaaa cccauuccac ugucaaagcu cuuuacacau     2700

cacuaccaga uagagcacau cauuccccag aguagguacu ucgacaauuc uagcuccaac     2760

aagaucauuu gugagucaga aguaaaugag gauaaagaca acaagaccgc uuuugaauau     2820

cugaaagaua agggggagaa ugugaucaac ggacauaaac uuuugagccu ggccgcauac     2880

gaggcccacg uuaauaagua cuucaagaac aaucgacaga aauuaaagaa ccuccugucu     2940

gaagacauac cugaggguuu caucaaccgc caaaugaaug auucccggua uauuaguaaa     3000

cuugucaagg gccuguugag caacaucgug agagaagagg gggaacagga ggugacuucu     3060

aaaaaucuca uuccagucac aggauccguu acgucaaagc ugaaaaacga cuggggccua     3120

aaugacaagu ggaacgagcu gauccucccg agguuuaagc gucugaauca gcuuacccaa     3180

acuaacgauu ucacaaccgc uaauacaaac gguaauacua uacccaccgu gccugacgaa     3240

uugcugaaag gguuuaguaa gaaacgcauu gaucaucggc accacgcguu agacgcacuc     3300

gugguagccu gcuguacgag aaaccauagc aauuaccugu cggcucuuaa caccgagaau     3360

aagaacuaug gccugaggga uaaacuacuc aucaagaaug acaacgauca cuacacaaag     3420

uccuuccaga ugccauggca uggauuuacu accgaagcca aaaaccagcu ggaggaaaca     3480

gucaucucuu ucaagcagaa uuugcgagug auuaacaaag caaauaacaa guuuuggagc     3540

uauaaagacg agaauggcaa gcugaacuua gauaagaaug ggaaacccau aaagaaacuc     3600

cggaagcaaa cuaaaggaga caacugggcc guucgccagc cucugcacga ggaaaccauc     3660

ucagguauug ugaaucuucc augggucaag gcugagaagg aaaaauucac guacgccaca     3720

agaaagaguc uggauaaauc cuuugacuug aagaaaaucg agaagauuac cgacacuggc     3780

auccagaaaa uacucaggaa cuaucugaug caggaaaagu ucaaggagcu agauaaaaag     3840

gacaaagagg uguacaauuc ugaacuggca uuuagccaag aggggcuuga agaucucaac     3900

uccaauaucg agcaguucaa cgaccagaag ccccauaaac cgauuuacaa gguucggcug     3960

uucgagaagg gaucaggccg auuugcguug gguuucaaag ggaucaaugc uaagaaauau     4020

gcccagggca gcccuaaccu guacuuuaac auuuauagaa augaagaugg acaauacuau     4080

gagacagugc cauuaaacaa gguaaucaua caccagaaaa augcagccaa caugcccaag     4140

aagaaucgca ccccuguccc cauuaguaac acugcuauca auaggucuaa agaagugcuc     4200

guggacuaca agaugacccu guccccacuu gaucuggucu auaucccuac agaggaagag     4260

uugaacaaua uuaacagccu cgacuucaau aaccugucga aaucacagcg uaagcggcua     4320

uacaauguua acgauuuuuc uggcuacacg aucuauuuca gucccaauac ccuggccaaa     4380

uccauaugcc caaaggaggu ggacaugaag uuugaugaga aaaagcagaa accgacuggg     4440

agcuucgacu cuaagacagc aagccuugau ggaaugucca uuaaagaaag auguaucaag     4500

cucauuaucg acaggcuggg caacauauca aaagcuuga                            4539


<210>  3433
<211>  4539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3433
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cguggucaau       60

gcugaugaga uaacaagaga cgaugguucc agguaccuga agcccaacgg caucucugca      120

gccgggucac ggauuauccc uaugagugcg gacauuuugg gagauuucga aaaaggcaau      180

agcaucuccc agacugcuga gcgcacgcga augcguaugg ccagaagguu acacgaacgg      240

gcacugcuac gcagagagag gcuccugcgg guucuuaacc uguuggacuu ucucccaaag      300

cauuucgccg auaaaauaga ccgcuauggu aaguuuaccg augacucuga accgaagcug      360

gcuuggcgaa agaauacaga ggggaaguac gaguucaucu uucaagaugc auucaacgaa      420

augcuggccg aguuuaaaga caagcagccc gaaauugugg aggagggcaa aaagaucccu      480

uaugauugga cuauuuacua ucucagaaag aaagccuuag aaaaggcccu gucgaaagag      540

gaacuuucau ggaucuugcu gcaguucaau cagaagaggg gauacuauca acuacggggc      600

gaggaagagg agauaccaca ggacaaaaag auugaauacc ucgcgcagaa aguagugaag      660

gucgaggcaa ccgaccagaa gaaaggggau gacguguggu acaacguuua ucuggaaaau      720

ggaaugaucu accgucgcac aagcaaggcu ccccuugauu gggaggguaa agugaaggaa      780

uuuauuguca ccacugaggu ggagaacgac ggcgaaacac cuaaaaagga uaaggagggg      840

aauaucaaaa gaaguuucag ggcuccaaag gaagacgauu ggacccuguu gaaaaagaaa      900

acggaggccg acaucgaaaa cucccacaag acuguaggau gcuauauuua cgauucucuc      960

cugaauaacc ccaagcagaa aauaaucggc aaguuaguuc ggaccgugga gcgaaaauuu     1020

uauaaggagg aacugacaca aauucucaaa aagcaggucg agcuuauccc ugaacugcgc     1080

gacgauaauu uguacaagca guguauagag gagcuauauc caauuaacga agcacauaga     1140

aacacuaucg cuaaaaccga cuucgccaau cuguuuauca acgacauucu cuucuaccaa     1200

aggccguuaa agagcaaaaa gucccagauc gauaauugcc ccuacgagga acggauuuuc     1260

auagacucaa aaacagguga gaagaaaaag gugccuguga aguguaucgc gaaaaguaac     1320

ccacuuuuuc aggaguuccg ccuguggcaa uuuauucaga auuugagaau cuaucagagg     1380

gaaaaggaga ucgaugggaa acuccugacc gacauugaua uaacgagcga augcuuaaag     1440

ucugaggaag acuacguccg ucuguucgau uggcuaaacg accgggagac uaucgaacag     1500

gaggaucucc ugaaacaccu uuuuaauaca aagaagucca aaaacaagga gaaucccuau     1560

cgauggaacu acguugaaga caaaguguau ccuuguaaug agaccagagc cacuauuuug     1620

aagggauucu caaaaugcgg caucaacgca agcguacuga guccagaacu cgagauggcu     1680

cuguggcaua uucuuuacuc uguggaagau aagaaggaga ucgagacagc ccugacccac     1740

uuugcucaaa aacagggcug gaaugacgau uucgccaagg ucuuuuccaa agcaaagccc     1800

uucaaaaagg acuaugggag cuacucggaa aaggccauca aaaaguugcu cucucugaug     1860

aggaugggaa aauacuggaa ccaggacaau auagauaaga acacguuaga ccgcauugau     1920

aaaaucauua acggugagua ugacgaaaag aucuccaauc gggugagaga uaacgcuaua     1980

aaucugaaag acauuucaga uuuuaggggc cuaccgguuu ggcucgcgug uuacaucgug     2040

uaugaccgcc acagugaggc aaaggauugc accaagugga acagcccuga ggaaauugac     2100

uccuaccuga aaaaguucaa acagcauucu cuucgaaauc ccaucgucga gcaaguggua     2160

acagaaacuu ugcggaccgu ucgugauaua uggauccagg aggaacagau ugacgagauc     2220

caccuggagc ucgggagaga ucugaagaac ccagccgaca aaaggaagaa gaugagcgaa     2280

aauauucuga aaaacgagaa uacaaaccug cgcaucaagg cuaugcuuau ggaauuuaug     2340

aauccuucaa ugggaauaga gaacgugcgg cccuauaguc caucccagca agacauuuug     2400

agaaucuacg aagagaaugc ccucgauaac cugacuaaag acgauaagga cuucgauuuc     2460

aucagcaaaa uuucuaagca ggcacagccc ucaaagaccg acaucauuag guauaaaugu     2520

uggcuagagc agaaguaccg aaguccuuac acaggcaaaa ccauaagccu guccaagcuc     2580

uuuacuccag ccuaugaaau cgagcauguc auuccgcaau cucuguacuu cgaugacucg     2640

uuuagcaaua aagugaucug cgaagcugag gucaacaaac uuaaggaucg ccaguugggu     2700

uauaaauuca uugccgaaca ccacggggag aaggugcagc ugucccaagg cgagguugug     2760

gaaaucuuau caguagacgc auacgagaaa uuugucaagg aaaacuaugc gaauaaccgg     2820

auaaaaauga agaagcuccu gauggagagu auccccgaug aguucauuga aagacagcuu     2880

aaugacucua gguacaucag caaagugguu aagggacugu uguccaacau ugugcgugag     2940

aaaaucgacg augaaaauua ugagccugaa gcugucucaa agaaccucau aagcuguaau     3000

ggcgccauua cggaccgccu gaaaaaggau ugggggauga acgacgugug gaacucuauc     3060

auccuaccac gguuuauuag aaugaaucag auaacaggaa aagauugcuu caccacuacc     3120

aaugcagagg gucaucugau cccccagaug ccucucgagc ugcaaaaggg cuuuaacaag     3180

aaaaggauug accaccgaca ucacgccaug gaugcuaucg uaauugccug cacaacccgg     3240

gaccauguga aucuuuugaa caaugaagca gcucacucca aguucaacgc cacucgcuac     3300

cagcugcaga gaaaauuaag gcgguuugag aaggcgguua ucgaugggaa agaacgagag     3360

gucgccaagg aguuccucaa gccaugggac aguuuuagcu cugauuccaa aaacauacug     3420

gaaaauauca uugugucauu caagcagaac caaagaguga ucaauaaaac aacgaacgcu     3480

uuccuucacu uugacgagaa uggaaagaaa accuucauua agcagggcaa gggugauagu     3540

ugggcaaucc gcaaaagcau gcauaaggac acugucuuug gggaaauaaa ccugaggaaa     3600

guucguucgg ugucccuaaa ugaggccauu aaggugcccg aacggguacu caacaaaaag     3660

aucaaggaga aaauccugga auugaagaau aacaaaauug acgcuaagaa uaucaaaaag     3720

uacauagagg agaaccacac aggcggauau ggcauugauu cuagcaaaau cgacgucuuc     3780

uacuuuacca aggaaacaaa ggagagauuc uuugccacua ggaaaacccu ggauacguca     3840

uucaaucagg caaagauuaa agacaguauc gcggauuccg ggauacagaa gauuuuacuc     3900

gcccaucuga aaucuaagaa cggagacgcu gaacaagccu uuagcccgga ugguaucgac     3960

gagaugaaua agaacaucgu ggagcuuaau aacggcaaau uccaccagcc uauucugaag     4020

guucgcgugu augaaaaagc agauaaguuu gcugucgggc agaaaggaaa caagaaggug     4080

aaauucguug aggccgcaaa gggcaccaau uuguucuuug ccaucuucga aaaagacggu     4140

aagcgauccu accucacaau uccacugaac gugaugauag auugucaaaa acaguauggg     4200

aaucagugga agaacaauau cgaggcuuac cuaaaggaaa aagagcuggu agagaaggac     4260

gucaaacuuc aguuuauucu cucacccaac gaucuggugu auuugccuac ugaaggcagc     4320

aucgacaaga aaagaaucua caaggugguc aguuucacca auaaccggcu guacgcgauu     4380

cccuaugcca uagcaaaauc uaucguugac aagaaugagu uuacacaauu aaacaagauu     4440

gaguucacug auaauaaaga auccaucaag gacgcuugcg ugccaguaau uaucgauagg     4500

cucggaaacg ugauagaauu uaauggcaaa cgccuguga                            4539


<210>  3434
<211>  4539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3434
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugggc cgugauaaac       60

uccgaggaag auggucccau cgucaaaaag agaauugagc uggcuggcuc uaggaucauu      120

ccuauggacg cagccguuuu ggggaauuuc gauucaggag ugacaaaaag ucagacugcg      180

gaacggacga gcuuucgcuc cauccgacgu uuaagagaga ggcaacugcu acggcgcgaa      240

agacuccaca agguacugca gauacuuggc uuccugccag agcauuacgu gaacgacauc      300

gauuuugaga aucacccggg uaaaauuaag aggaacaccg aacccaaguu gccuuggcgg      360

aaaaauucua uggggcaguu cgaguuuauc uuccaggacu cguuugaagc uaugcucaca      420

gauuucgcca cucaucaacc agucuuugug ucagagggaa agaaaguucc cuaugacugg      480

acccuguacu aucugcgcaa gaaagcacuc acagagggca uuagcaagga agaguuagcc      540

uggcugaucc uuaacuucaa ucagaagcga ggcuacuauc aguugagagg ggaagaggaa      600

gaggagcaga guaacaaacu gguggaauuu uacgcucuaa gggucgugaa gguagaggca      660

accgaugaca aaaagggaaa agauacuugg uauaauguuc accucgaaaa cgguuggaua      720

uaccggcgua cauccaauau uccucuggac ugggagggca aggugaaaga auuuaucguc      780

accacggauc uuaacgagga cgggacucca aagcuggaca aguacggaga ggugaaacgc      840

ucuuuuagag cccccaagga agaugacugg accuugauua aaaagaaaac agagagcgau      900

aucgcuaauu cccauaagac ugugggcugc uauaucuacg acgcccuccu gcaaaacccu      960

caccagaaga uuaaagguag guuaguccgg accguugaac gaaaguucua uaaagaggaa     1020

cugcgccuca uacuugagaa gcagcuguca uuccacccag aguugcagga uagagaccua     1080

uacagggcgu guaucgaugc acuguaugug aguaacgaag cccauaaaaa uaacauuagc     1140

gggcgggacu uuguauaccu cuucaucgau gacauaauuu ucuaucaacg cccgcugaag     1200

acaaagaaau cucuuaucga uaauugcccc uacgaggaaa accaguacau ggacagagag     1260

accggagagg ugaagcaggc uccuaucaaa uguauugcca agucccaccc acuguuccaa     1320

gaauuuaggu uguggcaguu caucucaaau auucguauau aucagcggga gcgaguccuc     1380

gacgauggca aacuggaaac ggacguggau guuacucccg acuuuuuaaa gacagaggau     1440

gacuacacca cucuguucga augguuuaac gauagaaagg agguggacca gaaagcauuc     1500

cuaaagaauc cucaauuugg ccucaaaaag acauucaccg aguauaggug gaacuacguc     1560

gaagauaaaa gcuauccaug caaugagacg cgcaacgcua uccugaaugg gcuucagaag     1620

gccggaguga gugcggacuu uuugucucgg gaaguagagg aagcccugug gcauauucuc     1680

uacuccguug aggauaaaau cgagaucgaa agcgcacuga gaaaguucgc uucgaagcac     1740

gaccuugaug acagguucgc cgacguguuu aaaaagcugc ccccguucaa aaaggaguau     1800

ggugcuuacu cugccaaagc aauuaagaag uugcucccuc ugaugcgcau aggcaaauac     1860

ugggaauuau ccaacaucga ugacaagacc aaagagcgaa uugauaagau cauuacaggg     1920

gaaugggacg agaauaucca cgaucggguc cgugagaaag ccauaaagcu gucagacauc     1980

acugauuuua agggacuacc ccucuggcug aguuguuaug uggucuacaa cagacauagc     2040

gaagcuaggg cgauugcaaa augggagucc ccagaagaca ucgauucuua ucuucgcuug     2100

uuccggcagc acagccugag aaauccuauu guggagcagg uuaucaccga gacacucagg     2160

acugugcgag acauauggaa gcgcguaggc caaauugaug aaauccaugu cgagcugggu     2220

cgggaaauua aaaaccccgc cgacaagaga gcuaaaauga ccgcccagau cguggagaac     2280

gaaaauacaa acuuaaggau aaaggcacug cuugccgagu uuaucaauuc agaguacgaa     2340

auugagaacg uucguccaua uaguuccaau cagcaggaaa aguugaaaau cuacgaggau     2400

accgugcuca acggggucaa ggaaauuccc gaggacauca auacuauacu gaaaaaguuc     2460

cgcgagagcg accuuaaaaa gcggccuacg aaaucugauu uucugcacua uaagcucugg     2520

cuggaacaaa aguacugcuc accauacaca ggcagaauua ucccgcuugg aaaauuguuc     2580

accagugcuu augagaucga acauauuauc ccccaggccc acuacuuuga cgauagcuuc     2640

uccaacaagg ugauuuguga ggcagaagua aauggccuga aagacaagaa auuaggguau     2700

gaguuuauaa agcacaaccc uggaaagaug guggagaucg guuucggcaa accacccguu     2760

aggaucuuuu cugucgaaga guacgaacga uucgugaagg agaauuauuc ggggauuaaa     2820

aacaagauga auaaacuccu gauggaugac auuccugaga cuuucaucga acggcagcuu     2880

aacgauagcc gcuacauauc caaggugguc aagucacugu ugaguaauau cguuagagag     2940

aaagacucua acggagaaua ugagccagaa gcgauuagca agaacgugau cgugugcacc     3000

ggcuccguaa cagauaggcu caaacgggac ugggguguca augaugugug gaacucaauu     3060

aucuaccccc gauuugagag acugaaugag cuaaccggga gccagcaauu cggcuguugg     3120

gaaaacaagg agggaaaacg cguuuuucag acugcugugc cgcuggaaau gcagaagggc     3180

uucucuaaga aaaggauaga ccaucgucac caugccaugg acgcaauugu caucgccugc     3240

gcuacacgga aucacaucaa cuaccucucc aaugagagug ccagcaagaa agcaaagauu     3300

ucuagaacgg aucugcaaag gcuuuugugu accaaaugcc gcacugacga uaacgggaau     3360

uauaagugga uagugaagaa accuuggcca gcuuuuacac aggacauccg aaaggcccug     3420

gaaaacauua ccguuuccuu caaacagaau uuaagaguga ucaacaagac aacuaauuac     3480

uauguacacu uugaugaguc agguaaaaag guccucgaga aacagauuaa cggagacagu     3540

ugggcgaucc ggaagcccau gcauaaggau accguguucg gcaaagugaa ucugcaaaag     3600

gucaaagaag uuaggcuuag ccuggcccua gacacgccua agaugauagu ggauaaaaag     3660

cucaaggaga aaguacugca guuguacucg uauaaguacg acaaaaagcg caucgauaaa     3720

uauuuuaagg aaaacgcuuc ccuguggaag gacuuaaacc ucucuaaagu ggcagucuac     3780

uacuucacca augauagcac agagccccug guggccguuc guaagccacu ugacacuuca     3840

uuuaacgcua aaaagauuaa agaaagugug accgacacag ggauccagaa gauucuguug     3900

aaucaccucu ccaacaauca ggaugacccu aaccuggccu ucucucccga uggaaucgac     3960

aagaugaaua aaaaccuaac ugcacugaau gauggcaagu aucaucaacc aauauacaaa     4020

gucagagugu gcgagccgca ggguaacaag uucagcguug ggaccuccgg caauaaaacg     4080

ucaaaguaug uggaggcggc caaaggaaca aaccuauuuu ucgcugucua ccagaccgaa     4140

gacggcaagc ggagcuauga gacuauuccu cucaauguag ugaucgaaag ggagaagcug     4200

ggguggacac ccguuccaga gaacaauaac aauggagaua aauugcuguu uugguuaagu     4260

cccaacgacc ucguguacgu cccuaccgcc gaugaacuga acaauggcau gcgccuugac     4320

aacauggaua agggucgaau cuauaaaaug auuucuuguu ccgggccaca gugcuucuuu     4380

aucccccaaa augugagcaa cgcaauaauu ccuguaaucg agcugggagc uaauaacaag     4440

gccgaaaaau cguggaauaa cgagaugauu aagguggucu guguuccgau cagaguggac     4500

cgguugggcu gcauaacuca gauuaauaac cagaaguga                            4539


<210>  3435
<211>  4551
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3435
augaagaaca uccugggccu cgaccuuggg accaauagca uuggaugguc cgugauacag       60

gagaaacaca acgaaaauaa cgagugccaa aagcugaucu ugggugccgg cucuagaauu      120

auccccaugg augcugcagu cuuaggggac uucgccaaag gaaauacaau uucacagacu      180

aaggauagga cgcaguaccg gggcguucgc cgacugcgug aaagaugucu acucaggcgg      240

gagcgccugu uuagagugcu ugacaucaug gguuuccugc cucagcauuu uaguaacgcg      300

uugaccaggu augggaaauu caaggaugcu gacgaggaac caaagauagc cuggcggaaa      360

gaugacgagg gaaagaugca cuuucucuuc gcagauagcu uuaaugaaau gaucgaggag      420

uuccgcuccg uacaaccgga acugcugaaa cgaggcauga aggugcccua cgacuggaca      480

cucuauuacu uaagaaagaa ggccaugagg gaggcuguca cuccuuuuga acuggcaugg      540

cuuauuuugu cuuucaacca gaagcggggc uauuaccagc ugcgugggga ggaagaggga      600

gaagccgaua auucgaaauc agaggaguau cuagcucagc gcgugguuga cgugaucgau      660

accggugaaa agaaaggcaa gagcacaugg uuugacauug uccucgagaa cgggauggug      720

uaccauagac cagccgaaau caaacccgac uggauaggaa agaccaaaga guucauugua      780

acuacacaau uugauaagga cggcaauccu aagaaagaua aggaagguaa cguuaaaaug      840

aguuucagga ugccaaauga gaacgacugg aaucugcaga agaaaaaggc ggagaaggau      900

aucgacgaau ccgggaugac cgugggagag uacauuuaua acgcacuucu gcacaacccc      960

aaacagaaga ucaaaggcaa guugguccgg gugguggaac gaaaauacua uaagaaugag     1020

cuccagaaga uccuggaauc ucaaaaacgc uucauuccuc aguuaagcga uuccgcccug     1080

uacgagaagu gcauaaaaga gcucuaucag ucaaacgagg cuuacagaaa uaguaucagc     1140

acgaggaacg acuuuacuua ucuuuucguc gauaauauua ucuuuuacca acggccacug     1200

aagucuaaaa aguccuugau agacaacugu ccguacgaau cacuagccua uaccaaggau     1260

ggcgaggaac auaaagcacc cguuaagugc auugcuaaaa gccacccucu guaccaggag     1320

uuccgccucu ggcaguuuau caguaaucug agaaucuauc agaggcaaaa acaggugggg     1380

gacaaaguaa aacuugacgu ggaugucaca gacgaguucc ugccaacuga ugaccgucgg     1440

gaaucuuugu uugauuaccu caacgacaaa accgaaauua agcaggaugu gcuguuaaag     1500

gaguucuuuu ccaugaaaaa gcccaaagga aaggacagcg uuuauccuua ccgauggaau     1560

uauguggaag auaaaucgua cccauguaac gucacaagag guaagauccu ggccaagcua     1620

aauagggcgg gcauuucugc cgacuuccuc uccucaaacg ggaaugagga gagucugugg     1680

cacauacuuu acagcgugga agauaaagga gaguugcgca aggcacuggc ucgguuugcc     1740

gcuaaaaacg gccucgacga ugccuucacc gaagcacugg ccgacaucga gcccuauaag     1800

aaagaauacg gugcuuauuc cgcgaaggca cuuaagaaac uguugccgcu caugagacug     1860

gggaaguacu ggaauuauga caacauugcc ccugcuacga aagccaggau cucuaagauc     1920

auugauggcg aguacgacga gaacauacgc aaucgaguac gggaaaaagc aaugagcuua     1980

aacucagcca gucaguuuca aggacugccc cuauggcucg cuugcuauau cguuuacgau     2040

cgucauuccg agagagccga cagcgggagg uggucuucac cagaagauau ugacaaguac     2100

cugagugauu ucaagcagca cagccuucgc aauccuaucg uggagcaggu cgugacugaa     2160

acauugcgga ccgucagaga cauuuggaaa agguaugggg agaucuccga gauacaugug     2220

gaacugggac gagagaugaa gaaccccgca gauaaacgcu cuaagaugaa ucggagaaug     2280

cucgaaaacg agaaugcgaa caugagggcu aaacuguuac ugacugaguu caugaaucca     2340

gaauuuggua ucgagaacgu ucguucguac agccccuccc agcaagaacu uuugcgcauu     2400

uaugaggacg gcgccaucuc aaaugcaggg agugaaauug augacgagau cucugauaua     2460

augagccggc ucucccaggu ggacguaaag aaacagccua cagccucaga ggucaagaga     2520

uacauucugu ggcuagaaca gaacuauagg agcccauaca ccggacaaau gaucccgcug     2580

gcuaaacucu ucacgcccgc cuaugagauu gaacacguga ucccucaguc ucgauacuuu     2640

gacgauuccu ucaguaauaa gguuauaugu gaggcagaag ugaacaagcu gaaagaccgg     2700

augcuuggcu acgaguuuau caagcagcac aaaggugaga aggucagcuu gucuaugggc     2760

ggcaccgugg aaauucuguc cccagaggaa uauguacgcc auguggaguc acaauacaag     2820

aauaacaaag cuaagaugag aaaauuacuc auggaugaca uccccgauga guucauuaac     2880

aggcagcuga augacagucg guauaucagc aaacuuauaa uuucgcuguu guccaacauc     2940

guucgagaag agggagaaga ggaagccaca ucuaagaaug ucaucagcug caacggcuca     3000

auuacugaua gacucaagaa ggacuggggg aucaaugaug uguggaacca cauuauacug     3060

ccucgcuuua ggcgucuaaa ugagcugacc ggaacagaca aauucacuac caguaacaag     3120

gagggucaug aagugccaga gaugccccuc gaacugcaga aaggcuuuaa uaagaaacgg     3180

aucgaucaca gacaucacgc gauggacgcc auugucaucg cuuguauuac aagggaccac     3240

guuaaccuuu ugaauaacga ggcagccaug uccaagaaua acgcuaaucg cauccagcug     3300

ucuaagaaau uacgaagaua cgaaagcgug gagauagaga aggaugggau ccggaaacaa     3360

auuuccaugg ccaaggaguu caaaaagccg ugggaaggau uuaccgcaga cguggaaagg     3420

acucuccgcg auaucguagu cucauucaag cagaaccugc gugugauuaa caaaacgaca     3480

aauuauuacc agcauuuuaa ggacggcaaa aagaucuaua gcccucagag uaaaggugau     3540

ucuugggcga uaagaaaguc caugcacaaa gagaccguuu ucggggaaau uaacauccgg     3600

aggaucaagg aggugagccu uaaggaggcc augaaacgca uuaaggacau agcugauaaa     3660

gaacugcgau cgaagaucgu cgagcuaaug gcccuccaau acaaugaaaa gaagauuaag     3720

caguacuucu cagacaacaa agaugagugg ucugacguga auccaaagaa aaucaagauu     3780

cauuauuuua cuaaagaaac caacgagaga uucuuugcaa cacggaagcc ccuggauacc     3840

aguuucgaca agaaaaagau caaaacucag guugcugaua cagccauaca gaagaucuug     3900

cugaaucacu uauccgcaca cggcgacaaa gccgagacgg cuuuuagccc ugacggaauu     3960

gaugaaauga acaggaauau cauugagcuc aacgacggca aguuccauca acccauccug     4020

aaggugcgca uauacgaaaa agcggauaag uaugccguag ggcagcaggg aaauaaaaag     4080

aaaaaguacg ucgaggcagc uaaaggcacc aaccuguuuu ucgccauuua ugccgaggaa     4140

aagaucgacg agaagacugg ugauacagug aucaccagaa cauacgacuc uauuccacuu     4200

aaugugguca ucgauaggga aaaacggggg uugagcccug uucccgagaa cgaaaaugga     4260

gcuaagccaa aauaugugcu cuccccgaac gaccugguau acgugccuaa ugaggaggaa     4320

cgugagacug gccgcauaug cgaaccccua gauaaggagc gaauuuacaa aauggucuca     4380

aguaacaaga agcaagcauu uuucaucaaa agcgaagugg ccucuaccau uguugacaag     4440

uuugaguucu ccgcgcugaa caaacaggag agagcucuuu caggugaaau gaucaaggag     4500

auauguauuc caaucaguau caauaggauu ggcgaaauca cgcuccggug a              4551


<210>  3436
<211>  4554
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3436
augaagaaaa ucgugggccu ggaccucggg accaacagca uuggaugggc ccuuauaaau       60

gcuuacauca acaaggagca ccuguauggu auugaagcau gcggcuccag aaucauuccc      120

auggaugccg cgaucuuggg gaauuucgac aaaggaaacu cuauaucaca gacagcugau      180

aggacgaguu accggggcau ccgccgauua cgugagagac aucugcuaag gcgggaacgc      240

cuccacagga uucuggaccu ucuggguuuc uugccuaagc auuauagcga uucccucaau      300

agauacggga aauuccugaa cgacaucgag uguaagcugc caugggucaa ggaugaaacu      360

ggaucuuaua aauuuauuuu ccaagagucg uuuaaggaga ugcucgccaa uuucaccgaa      420

caccauccga ucuuaauagc aaacaauaaa aagguucccu acgacuggac aauuuauuac      480

cugcggaaaa aggcccuuac ucagaagauc ucaaaagagg aauuggcuug gauucugcua      540

aacuuuaauc agaagcgcgg cuacuaucag cuccgaggcg aggaggaaga gaccccuaac      600

aaacuggugg aauacuauag ccuuaaggua gagaaagugg aagauagugg ggagagaaag      660

ggaaaagaca caugguacaa uguccaccug gagaacggua ugaucuauag gcggaccucc      720

aauaucccau uggauuggga aggcaagacu aaggaguuca uugugacaac cgaccucgaa      780

gcagaugggu cucccaaaaa ggacaaagag ggaaacauaa agcguagcuu ucgcgccccu      840

aaagacgaug acuggacgcu gaucaagaag aaaacugaag cugauauuga caagaucaaa      900

augaccguug gcgccuacau auaugauaca uuacugcaaa agccagacca gaaaauuaga      960

gguaagcucg ugaggacuau cgagcggaag uacuacaaaa acgagcuuua ucagauccug     1020

aagacccagu ccgaguucca cgaagaauug cgagauaaac aacuauacau ugcgugccug     1080

aaugagcucu aucccaacaa ugaaccucgc agaaacucaa ucaguacaag ggacuuuugu     1140

caucuguuca uugaggauau aaucuuuuac cagcggccac uuaagagcaa aaagucucug     1200

auugacaauu gcccguauga ggaaaaccgc uacaucgaua aggaguccgg ggaaaucaaa     1260

cacgcaucaa uuaaguguau agccaaaagc caucccuugu aucaggaguu cagacucugg     1320

caguucaucg ucaaucugag gauuuaccgu aaggagaccg acguggacgu aacgcaagaa     1380

uuacugccua cugaggcuga uuacguuaca cuauuugaau ggcucaacga gaaaaaggaa     1440

aucgaccaga aagccuucuu uaaguaucca cccuucggau uuaagaaaac cacaaguaau     1500

uaccggugga acuaugugga ggauaagccu uacccaugca augagacuca cgcacagauu     1560

aucgcucgac ugggcaaagc ccacauaccc aaggcguucc uuucuaaaga aaaggaggaa     1620

accuuguggc auauccugua uuccauugag gacaagcaag aaaucgagaa agcccuccac     1680

agcuuugcaa acaagaauaa ccugucggag gaguucauug aacaguuuaa aaauuucccg     1740

ccuuuuaaga aagaauacgg cucuuauucc gcuaaggcca ucaagaaacu ucugcccuug     1800

augagaaugg ggaaguacug gucaauagag aacauugaua acggaacgag gauccgcauu     1860

aauaaaauca uagacgguga auacgaugag aacauccggg agagagucag gcagaaggcu     1920

auuaaucuca ccgacaucac acauuuccgc gcccugccau uauggcuggc auguuaucua     1980

guguacgauc gacacaguga agugaaagac auugucaagu ggaagacucc uaaagauauc     2040

gaccucuauc uuaagagcuu uaaacagcau ucccuucgga accccauagu ugagcaagug     2100

auuaccgaaa cauugcguac uguaagagau aucuggcaac aggugggcca caucgacgag     2160

auucacaucg agcuggggag ggaaaugaag aauccagccg acaaacgcgc ucggaugucu     2220

cagcagauga uuaagaacga gaauaccaac cucagaauaa aagcgcuguu aacagaauuu     2280

cugaaucccg aguucggaau cgaaaacguc aggccuuaca gcccaucaca gcaggaucuu     2340

uugcgaauuu augaggaggg cgugcucaau aguauccugg aacuaccgga ggacauuggu     2400

aucauacugg ggaaguuuaa ccagaccgau acucucaagc gccccacgcg guccgaaauc     2460

cugagauaca aacuuugguu ggagcaaaag uauaggagcc cuuacacagg cgaaaugauu     2520

ccacugucua aauuauucac ccccgcauac gagaucgagc auauuauccc ucagucacgu     2580

uauuuugacg auagucucag caauaagguu auaugcgaau ccgagauuaa caaacugaag     2640

gaccgcucuc uuggauacga guucaucaag aaucaccaug gcgaaaaagu ggaacuggcc     2700

uuugauaagc cagucgaggu guugucggua gaggcuuaug aaaaacucgu ucacgagagc     2760

uacucccaca accggucaaa gaugaaaaag cugcuaaugg aagacauccc cgaucaguuc     2820

auugagagac agcugaacga caguagguau auaucuaagg uggucaaaag ccuccugucc     2880

aauaucgugc gagaggaaaa cgagcaagaa gccauuucaa agaaugucau cccguguacu     2940

gggggaauua ccgaucggcu uaaaaaggac ugggguauca acgaugugug gaauaaaaua     3000

guuuugccuc gcuuuaucag acugaacgag uuaacagaaa gcaccagguu cacuucuauu     3060

aauacaaaca auacgaugau cccauccaug ccccucgagc ugcagaaggg cuuuaacaag     3120

aaacggauug accaucgaca ccaugcaaug gacgccauca uaauugcuug cgccaauaga     3180

aacaucguga auuaccuuaa caauguaagu gcaagcaaga acaccaaaau cacucgcagg     3240

gaucugcaga cacuacucug ucacaaggac aaaaccgaua acaaugggaa cuauaagugg     3300

gucauugaca agccuuggga gacauucacu caagauaccc ugacggcguu gcagaaaauc     3360

accgugucuu uuaagcagaa ucugcguguu auaaacaaaa caacuaauca uuaccagcac     3420

uacgaaaacg gaaagaaaau uguguccaau caaucaaagg gcgacaguug ggcuauccgg     3480

aaaagcaugc acaaggagac cguccauggu gaagugaacu uaagaaugau uaagacagua     3540

ucguucaaug aggcccucaa aaagccccag gcaaucgugg aaauggaucu gaaaaagaaa     3600

auacuugcca ugcuggaguu gggguaugac acuaagagga uuaagaacua cuuugaggaa     3660

aauaaagaua ccuggcagga caucaaccca uccaagauca aaguuuauua cuucacgaag     3720

gagacaaaag aucgcuauuu cgcuguccga aagccuauug acaccucuuu ugacaagaaa     3780

aagaucaaag aaagcauuac ugauacaggc auacagcaaa ucaugcucag acaccuggag     3840

accaaggaca augaucccac ucuagccuuc ucaccagacg gaauugauga aaugaaccgg     3900

aauauccuga uccuuaacaa aggcaagaag caucagccga uuuacaaagu gaggguguau     3960

gagaaggcag agaaauuuac cgucgggcag aagggaaaua aacgcacaaa guucguugaa     4020

gcugccaaag guacgaaccu cuuuuucgcg auauacgaga ccgaagagau cgacaaggau     4080

acuaagaaag ugauucguaa gagaaguuac uccacaaucc cucugaacgu gguaauugag     4140

cggcagaaac aaggcuuguc uagcgccccc gaagacgaga augggaaccu gccaaaguau     4200

aucuuauccc ccaaugaucu cgucuacgug ccuacccagg aagagauaaa caaaggagaa     4260

guugugaugc caaucgacag ggaucgcauu uauaagaugg ucgacucaag cggcaucaca     4320

gcuaauuuua uucccgcaag uacugccaac cugaucuucg cucugccuaa ggccaccgca     4380

gagauauacu gcaaugguga gaacugcauu cagaaugaau augggaucgg cucuccgcaa     4440

uccaaaaacc agaaggcgau uacgggagag auggugaaag aaaucuguuu uccaauaaag     4500

guugaucgac ugggcaauau cauucaggug gggagcugca ucuugacaaa cuga           4554


<210>  3437
<211>  4563
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3437
augaagaaaa ucgugggccu ggaccucggg accaacagca uuggaugggc ccuuauaaau       60

gcuuacaucg augaguauaa caaggaacac cuguacggua uugaggcaug cggcuccaga      120

aucauuccca uggacgccgc gaucuugggg aauuucgaua aaggaaacuc uauaucacag      180

acagcugaca ggacgaguua ucggggcauc cgccgauuac gugaaagaca ucugcuaagg      240

cgggagcgcc uccacagaau uuuggaucuu cuggguuuuc ugccuaagca uuacagcgac      300

ucccucaaua gguaugggaa auuccugaac gauaucgaau guaagcugcc augggucaag      360

gacgagacug gaucuuacaa auuuauuuuc caagagucgu uuaaggaaau gcucgccaau      420

uucaccgagc accauccgau cuuaauagca aacaauaaaa agguucccua ugauuggaca      480

auuuacuacc ugcggaaaaa ggcccuuacu cagaagaucu caaaagaaga guuggcuugg      540

auucugcuaa acuuuaauca gaagcgcggc uauuaccagc uccgaggcga ggaagaggaa      600

accccuaaca aacuggugga guauuacagc cuuaagguag aaaaagugga ggacaguggg      660

gagagaaagg gaaaagauac augguauaau guccaccugg aaaacgguau gaucuacagg      720

cggaccucca auaucccauu ggacugggag ggcaagacua aggaguucau ugugacaacc      780

gaucucgaag cagacggguc ucccaaaaag gacaaagaag gaaacauaaa gcguagcuuu      840

cgcgccccua aagaugacga uuggacgcug aucaagaaga aaacugaggc ugacauugau      900

aagaucaaaa ugaccguugg cgccuauaua uacgacacau uacugcaaaa gccagaucag      960

aaaauuagag guaagcucgu gaggacuauc gagcggaagu acuauaaaaa cgaacuuuac     1020

cagauccuga agacccaguc cgaguuccac gaagaguugc gagacaaaca acuauauauu     1080

gcgugccuga augaacucua ccccaacaau gagccucgca gaaacucaau caguacaagg     1140

gauuuuuguc aucuguucau ugaggacaua aucuuuuauc agcggccacu uaagagcaaa     1200

aagucucuga uugauaauug cccguacgaa gagaaccgcu auaucgacaa ggaauccggg     1260

gagaucaaac acgcaucaau uaaguguaua gccaaaagcc aucccuugua ccaggaguuc     1320

agacucuggc aguucaucgu caaucugagg auuuaccgua aggaaaccga cguggaugua     1380

acgcaagagu uacugccuac ugaagcugac uauguuacac uauuugagug gcucaacgag     1440

aaaaaggaaa ucgaucagaa agccuucuuu aaguacccac ccuucggauu uaagaaaacc     1500

acaaguaauu aucgguggaa cuacguggag gacaagccuu auccaugcaa ugaaacucac     1560

gcacagauua ucgcucgacu gggcaaagcc cacauaccca aggcguuccu uucuaaagag     1620

aaggaagaga ccuuguggca uauccuguac uccauugaag auaagcaaga gaucgagaaa     1680

gcccuccaca gcuuugcaaa caagaauaac cugucggaag aguucauuga acaguuuaaa     1740

aauuucccgc cuuuuaagaa agaguauggc ucuuacuccg cuaaggccau caagaaacuu     1800

cugcccuuga ugagaauggg gaaguacugg ucaauagaaa acauugacaa cggaacgagg     1860

auccgcauua auaaaaucau agauggugag uaugacgaga acauccggga aagagucagg     1920

cagaaggcua uuaaucucac cgauaucaca cauuuccgcg cccugccauu auggcuggca     1980

uguuaccuag uguaugaccg acacagugag gugaaagaua uugucaagug gaagaucccu     2040

aaagacauag aucucuaccu uaagagcuuu aaacagcauu cccugcggaa ccccauuguu     2100

gaacaaguga ucacugagac cuugcguaca guaagagaca ucuggcaaca ggugggccac     2160

auugacgaga uccacauuga acuggggagg gagaugaaga auccagccga uaaacgcgcu     2220

cggaugucuc agcagaugau aaagaacgaa aauacuaacc ucagaaucaa agcgcuguua     2280

accgaguucc ugaaucccga guucggaauu gaaaacguca ggccuuauag cccaucacag     2340

caggaccuuu ugcgaaucua cgaggaaggc gugcucaaua guauucugga gcuaccggaa     2400

gauaucggua uaauccuggg gaaguuuaac cagacagaca cccucaagcg ccccacucgg     2460

uccgagauuc ugagauauaa acuuugguug gaacaaaagu acaggagccc uuacacgggc     2520

gagaugaucc cacugucuaa auuauucaca cccgcauaug agauugaaca uaucauaccu     2580

cagucacguu acuuugauga cagucucagc aauaagguua uuugcgaguc cgaaaucaac     2640

aaacugaagg aucgcucucu uggauaugag uucaucaaga aucaccaugg cgaaaaagug     2700

gagcuggccu uugacaagcc agucgaggug uugucgguag aggcuuacga aaaacucguu     2760

cacgaaagcu auucccacaa ccggucaaag augaaaaagc ugcuaaugga ggauauuccc     2820

gaccaguuca uagagagaca gcugaacgau aguagguaca ucucuaaggu ggucaaaagc     2880

cuccugucca auauugugcg agaagagaac gaacaagagg ccaucucaaa gaaugucauu     2940

ccguguaccg ggggaaucac ugaccggcuu aaaaaggauu gggguauaaa cgacgugugg     3000

aauaaaaucg uuuugccucg cuuuauuaga cugaacgaau uaaccgagag cacaagguuc     3060

accucuauca auacuaacaa uacaaugauu ccauccaugc cccucgagcu gcagaagggc     3120

uuuaacaaga aacggaucga ccaucgacac caugcaaugg augccauaau uaucgcuugc     3180

gccaauagaa acaucgugaa uuaucuuaac aauguaagug caagcaagaa cacgaaaauu     3240

acccgcaggg accugcagac ucuacucugu cacaaggaua aaacagacaa caaugggaac     3300

uacaaguggg ucaucgauaa gccuugggaa accuucacac aagacacucu gaccgcguug     3360

cagaaaauaa cggugucuuu uaagcagaau cugcguguua uuaacaaaac cacaaaucau     3420

uaccagcacu augagaacgg aaagaaaauc guguccaauc aaucaaaggg cgauaguugg     3480

gcuauucgga aaagcaugca caaggaaacu guccauggug aggugaacuu aagaaugauc     3540

aagaccguau cguucaauga agcccucaaa aagccccacg caauagugga gauggaccug     3600

aaaaagaaaa uucuugccau gcuggaguug ggguacgaua caaagaggau caagaacuau     3660

uuugaagaga auaaagacac uuggcaggau aucaacccau ccaagauuaa aguuuacuau     3720

uucaccaagg aaacgaaaga ccgcuacuuc gcuguccgaa agccuaucga cacaucuuuu     3780

gauaagaaaa agauuaaaga gagcauaacc gacacuggca uccagcagau uaugcucaga     3840

caucuggaaa caaaggauaa ugaccccacc cuagccuucu caccagaugg aaucgacgag     3900

augaaccgga auauccugau ucuuaacaaa ggcaagaagc accaaccgau auauaaagug     3960

aggguguacg agaaggcaga aaaauuuacu gucgggcaga agggaaauaa acgcaccaag     4020

uucguugagg cugccaaagg uacaaaccuc uuuuucgcga ucuacgaaac ggaggagauu     4080

gauaaggaca ccaagaaagu gauccguaag agaaguuauu ccacuauucc ucugaacgug     4140

guaaucgaac ggcagaaaca gggcuugucu agcgcccccg aggaugaaaa ugggaaccug     4200

ccaaaguaca uauuaucccc caaugaccuc gucuaugugc cuacacaaga ggaaaucaac     4260

aaaggagagg uugugaugcc aauugauagg gaccgcaucu acaagauggu cgauucaagc     4320

ggcauuaccg cuaauuuuau ccccgcaagu acagccaacc ugauauucgc ccuuccuaag     4380

gcuacugcag agauuuauug caauggugaa aacugcaucc agaaugagua cgggauuggc     4440

ucuccgcagu ccaaaaacca aaaggcgauc accggagaaa uggugaaaga gauauguuuu     4500

ccaaucaagg uugaccgacu gggcaauauu auccaggugg ggagcugcau uuugacgaac     4560

uga                                                                   4563


<210>  3438
<211>  4563
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3438
augaagaaaa ucgugggccu ggaccucggg accaacagca uuggaugggc ccuuauaaau       60

gcuuacaucg augaguauaa caaggaacac cuguacggua uugaggcaug cggcuccaga      120

aucauuccca uggacgccgc gaucuugggg aauuucgaua aaggaaacuc uauaucacag      180

acagcugaca ggacgaguua ucggggcauc cgccgauuac gugaaagaca ucugcuaagg      240

cgggagcgcc uccacaggau ucuggaucuu cuggguuucu ugccuaagca uuacagcgac      300

ucccucaaua gauaugggaa auuccugaac gauaucgaau guaagcugcc augggucaag      360

gacgagacug gaucuuacaa auuuauuuuc caagagucgu uuaaggaaau gcucgccaau      420

uucaccgagc accauccgau cuuaauagca aacaauaaaa agguucccua ugauuggaca      480

auuuacuacc ugcggaaaaa ggcccuuacu cagaagaucu caaaagaaga guuggcuugg      540

auucugcuaa acuuuaauca gaagcgcggc uauuaccagc uccgaggcga ggaagaggaa      600

accccuaaca aacuggugga guauuacagc cuuaagguag aaaaagugga ggacaguggg      660

gagaagaaag gaaaggauac augguauaau guccaccugg aaaacgguau gaucuacaga      720

aggaccucca auaucccauu ggacugggag ggcaagacua aagaguucau ugugacaacc      780

gaucucgaag cagacggguc ucccaagaaa gacaaggaag gaaacauaaa acggagcuuu      840

cgugccccua aggaugacga uuggacgcug aucaagaaaa agacugaggc ugacauugau      900

aaaaucaaga ugaccguugg cgccuauaua uacgacacau uacugcaaaa accagaucag      960

aagauucgcg guaagcucgu gagaacuauc gagaggaaau acuauaagaa cgaacuuuac     1020

cagauccuga aaacccaguc cgaguuccac gaagaguugc gggacaagca acuauauauu     1080

gcgugccuga augaacucua ccccaacaau gagccucgac gcaacucaau caguacaaga     1140

gauuuuuguc aucuguucau ugaggacaua aucuuuuauc agaggccacu uaaaagcaag     1200

aagucucuga uugauaauug cccguacgaa gagaaccggu auaucgacaa agaauccggg     1260

gagaucaagc acgcaucaau uaaauguaua gccaagagcc aucccuugua ccaggaguuc     1320

cgccucuggc aguucaucgu caaucugaga auuuacagga aagaaaccga cguggaugua     1380

acgcaagagu uacugccuac ugaagcugac uauguuacac uauuugagug gcucaacgaa     1440

aagaaagaga ucgaucagaa ggccuucuuu aaguacccac ccuucggauu uaaaaagacc     1500

acaaguaauu aucguuggaa cuacguggag gacaaaccuu auccaugcaa ugaaacucac     1560

gcacagauua ucacccggcu gggcaaggcu cacauaccca aagccuuccu uucuaaggag     1620

aaggaagaga cguuguggca uauccuguac uccauugaag auaaacaaga gaucgagaag     1680

gcgcuccaca gcuuugccaa caaaaauaac cugucggaag aguucauuga acaguuuaag     1740

aauuucccgc cuuuuaaaaa ggaguauggc ucuuacuccg caaaggcuau caaaaagcug     1800

cugcccuuga ugcgaauggg gaaauacugg ucaauaaaga acauugacaa cggaaccaga     1860

aucaggauua auaaaaucau agauggugaa uaugacgaga acauccgcga gcgggucaga     1920

cagaaggcca uuaaucucac agauaucacu cauuucaggg cucugccauu auggcuggcc     1980

uguuaccuag uguaugaccg ccacagugaa gugaaggaua uugucaaaug gaagaccccu     2040

aaagacaucg aucucuaccu uaagagcuuu aaacagcauu cccuucgaaa ccccauaguu     2100

gagcaaguga uuacagaaac uuugcggacc guacgugaca ucuggcaaca ggugggccac     2160

aucgacgaga uucacaucga gcuggggaga gaaaugaagu cuccagcaga uaaaagggcc     2220

cgcaugagcc agcagaugau uaagaaugag aacacaaauc uccggauaaa ggcucuguua     2280

accgaauuuc ugaaccccga guucggaauc gaaaauguca gaccuuauuc accaagucag     2340

caggaccuuu ugaggauuua cgaggagggc gugcucaacu ccauccugga acuaccggag     2400

gauauuggua ucauacuggg gaaauuuaau cagacugaca cgcucaagcg acccacacgc     2460

agcgaaaucc ugcgguauaa acuuugguug gagcaaaagu acagaucucc uuacaccggc     2520

gaaaugauuc cacugucaaa auuauucacu cccgcguaug agaucgagca uauuaucccu     2580

cagaguaggu acuuugauga cagccucucc aacaagguua uaugcgaauc ugagauuaau     2640

aagcugaaag aucguucgcu uggauaugag uucaucaaga accaccaugg cgaaaaagug     2700

gaacuggcau uugacaagcc agucgaggug uugagcguag aggccuacga aaaacucguu     2760

cacgaguccu auucacacaa ucgcaguaag augaagaaac ugcuaaugga agauaucccc     2820

gaccaguuca uugagcggca gcugaacgau ucuagauaca uaagcaaggu ggucaaaucc     2880

cuccugucaa acaucgugag ggaggaaaau gagcaagaag cuauuagcaa gaacgucauc     2940

ccguguaccg ggggaauuac agaccgacuu aaaaaggauu gggguaucaa ugacgugugg     3000

aacaagauag uuuugccucg guuuauccgc cugaaugagu uaaccgaauc uacuagauuc     3060

acauccauua acacgaauaa caccaugauc ccaaguaugc cccucgagcu gcagaaaggc     3120

uuuaauaaga aaaggauuga ccaucggcac caugccaugg augcaaucau aauugccugc     3180

gcuaaccgaa auaucgugaa cuaucuuaau aacguaagcg ccucuaagaa uacuaaaauc     3240

acaagacgcg accugcagac ccuacucugu cacaaggaua agacagacaa caacgggaau     3300

uacaaauggg ucauugauaa gccuugggag acuuucaccc aagacacgcu gaccgcauug     3360

cagaaaauca caguguccuu uaagcagaac cugaggguua uaaauaaaac uaccaaccau     3420

uaccagcacu augaaaaugg aaagaaaauu gugucaaacc aaaguaaggg cgauagcugg     3480

gcgauccgua agucgaugca caaagagaca guccauggug aagugaauuu acggaugauu     3540

aagacuguau cccucaacga ggcucugaaa aagccccagg ccaucgugga aauggaccuu     3600

aaaaagaaga uacuggcaau guuggagcuc ggguacgaua ccaaaagaau uaagaauuau     3660

uucgaggaaa acaaagacac guggcaggau aucaauccau cuaagaucaa aguuuacuau     3720

uuuacaaagg agaccaagga cagguacuuc gccguccgca aaccuauuga cacuagcuuc     3780

gauaagaaaa agaucaaaga aucaauuaca gacaccggca uacagcaaau caugcugcga     3840

caccuagaga cuaaggauaa cgaccccacc cuggcuuuua guccagaugg aauugacgaa     3900

augaauagaa acauccuuau ccucaauaag ggcaaaaagc aucagccgau uuauaaagug     3960

cggguguacg agaaggccga gaaauucaca gucgggcaga agggaaacaa aaggacgaag     4020

uuuguugaag cagcuaaggg uaccaaucug uucuuugcca uauacgagac ugaagagauc     4080

gauaaagaca caaagaaagu gauucgcaag cguuccuauu cuaccauccc uuugaacgug     4140

guaauugaga gacagaaaca aggccugagc uccgcgcccg aagaugagaa cgggaauuua     4200

ccaaaguaca uccucucacc caacgaccug gucuaugugc cuacacagga agagauaaau     4260

aagggagaag uugugaugcc aaucgaucgg gacaggauuu acaaaauggu cgauagcagu     4320

ggcaucacug ccaacuucau ucccgcuucu accgcaaauc uuaucuuugc ccugccuaag     4380

gcuacggccg agauauauug caacggugag aauugcauuc agaacgaaua cgggaucggc     4440

uccccgcaaa gcaaaaauca gaaggcaauu acaggagaga uggugaaaga aaucuguuuc     4500

ccaauaaagg uugaccgcuu gggcaacauc auucaggugg ggucgugcau ccucaccaau     4560

uga                                                                   4563


<210>  3439
<211>  4575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3439
augaacaaga uccugggccu cgaccuuggg accaauagca uuggaugggc cauagugcag       60

aaagaugagc aagguuucua cgaaaagauc auuaacgcug gcuccagaau cauucccaug      120

gacgcagaga caaugaauaa cuuuaauaaa gggaucacua agucuccugc cucagaaagg      180

acgcgguucc gcggagcgcg acgucugaua gagagaaguu uguuaaggcg ggaacgcauc      240

cacagacugc uaaacaccau gaauuaucuc ccaccgcauu acgcugagaa aauugauuuu      300

gucaacaggc ugggcaaguu caucggugag gaggaaccca aguaugccua caaaguugac      360

gaacacggga aguuucaguu cauuuuuaaa gagagcuuca augagaugcu uaaggauuuu      420

caggacaaac agccugaacu gguguugaac aauaagaagg uaccauauga uuggacaauc      480

uacuaucucc ggaaaaaggc acugaaccgc gccauaucca aagaggaacu gggauggauu      540

auccuccaau ucaauaacaa gcgaggcuac uaccaguuaa gaggcgagga agacgagacu      600

cugaccgagg ggcagaaaaa ggaauauuuu gcucuuaaag ugauuagggu cgaggcagau      660

ucuucgucag ccggaaagaa gggugaagug ugguacaaug uucaguugga gaacggcugg      720

aucuaucggu gcaaaagcga aaucccccug gacugggagg ggaagauuaa agaguucaua      780

gugacaaccg aagucgauga gaacgacaau gugcuaaagg auaaagaagg aaaggagaag      840

cguaaacuca agaucccuga agcuggcgac uggacucugc uuaaaaagaa aacagagagu      900

gaccuggaua aguccgguaa gaccguaggg gccuacauuu augacacguu gcucucuaac      960

ccagaucaga aaaucaaggg aggccugaua cgcacuauug agagagaauu auacaggagc     1020

gagcuggaac aaauccucga caaacagaag gaguuucauc ccgagcuuaa agauggcacc     1080

cuguauaaua aguguguuga agaguuguac cgguccaacg aaucacgacg caucagucua     1140

agcaauuacg acuucacaag auucauuacu gaggauguga ucuuuuauca gaggccucug     1200

aagucuaaaa agucccucau ugacaacugc ucauucgaac ggcgcaauua ccugaaccca     1260

auaaccaaag agcuugcgua ugcaccgauc aaguguauug ccaaaagcca cccccuguuu     1320

caagaguuca gauuguggca guuuaucaag aaucucagga ucauugaacg ugagaaaaua     1380

gucggggaua agcugauguu cgaccaggau gugacaaccg aauuuuuacc uacgcaaaac     1440

gacuacguga uccuguucga cuggcuaaau gagcauaagg aaauugauca gaaagcucuc     1500

cugaaguauc cagccuuuaa ccuuaaaaag aauaucgaca aauaccggug gaacuauguc     1560

gagaauaaga guuaccccug caacgagacu cgagcauugc ugaaggcuaa acucaacaag     1620

gccggaaaua uuccuucuga uuuccuggac aacgauacac uugaaucccu guggcacauc     1680

uuguacagcg uugaggacaa auuugaaaua gagaaggcgc ucaaaucguu cgccaccaag     1740

aaggaacuga gagaggagca gacuguggca uuuguaaaac aguucaucaa uauuccaccc     1800

uucaagaaag aauaugguuc uuacuccgcu aaggccauca aaaaguuacu gcaacuaaug     1860

aggaugggcu cacgcuggag ugagaacgaa auuagcgauu ccacaaagga ccggauccag     1920

aaacucauag auggggagug ggacgagucu auuaagacca gaacgaggga uaaagcuauc     1980

aagcugaaug aaauuaacga cuuucaggga cuuccguugu ggcuggccag cuauaucgug     2040

uacgaucgcc acucagagag uggcgacguc augaaauggg aaaccccuga ggacauagau     2100

uauuaccuca agcauguguu caaacagcac ggucugcgaa aucccaucgu ugaagugguc     2160

gugguagaga cauuaaaggu ugugaaggac cuguggaaaa cuuaugggua cuuuaacgag     2220

auucauaucg aacuuggccg ggagaugaag aauccagcag auaaacguag auccaugacc     2280

aacaagaaua uugaaaacga gaauacaaac uugaggauca aagcccuccu ggcugaacua     2340

gcgaaucaca aggacgucga gggagugcgc ccuuacagcc ccucucaaca ggagauacug     2400

aagauuuaug aagagggcgu ccucgauaug cugacuaaag aagacccaga uuacgaguca     2460

aucaguaaga ucagcaaaau ggcacuuccc uccucuucgg aauugauucg guauaagugu     2520

uggcuggagc agaaauacag aagcccuuau accggacagg ugaucccauu agcccgccuc     2580

uucacauccg aguacgaaau ugagcacaua aucccgcaau cacgauauuu ugacgauagu     2640

uucucuaaca agguuauuug cgaaagcgcu gugaacaagg acaaagauaa ucugcuugcc     2700

uacgaguaca ucaagcagaa cgagggaaaa auuaucgaaa uaggucuggg caagaaagua     2760

aaguuguuua ccgcagacuc cuauacugag uucguccagu caaauuacgu ggggagcguu     2820

gccaagaaaa agaaacuccu gauggauacg auccccgacu cuuuuauuaa gcgccagcua     2880

aacgacuccc gguauaucag uagagaaauu augaaacugc ucagcucugu ggucagggag     2940

aaggaagagg aggaagagga agaggaagag gaggcuaucu ccaagaaugu gauaguaugu     3000

acaggagcca uuaccgauaa acugaagcgu gaauggggcc uuaacgacgu guggaauacu     3060

aucaucuacc cucgcuucga gcgguugaac caacugacca auucagauaa auuuggucag     3120

ugggaaaaca agcaggggaa aaagguuuuc caaauugaga ugccauuaaa acuccagaag     3180

ggcuuuaaua agaaaagaau agaccauagg caccaugcaa uggaugcgau cgucauugcu     3240

ugcgccacac agagucacgu gaacuaucug aauaacgaaa gcgcacauuc caauucgaag     3300

gagaaacgau acuaucuucg gcgcaagcug agaaggaucg agauucuaga aaaacaggag     3360

cucaaggacg gagugaccau cacuaagaaa auagaagucg ccaaggaguu caacaaaccc     3420

uggccuacau ucacgcaaga ugcucacgag guucugcagu cuaucauugu gagcuuuaag     3480

cagaacuugc gggugguaaa uaaagccacc aaccgauacg cauguuucgu caauggcaag     3540

aaggaaaucg ugaaacaguc aaagggggag aguugggcua uuagaaaacc aaugcacaag     3600

gacacuguuu ccgccgcggu gucucugcgc aaaaucaaga cagucagcuu aucccaagcc     3660

auagaugacu ggaccaacau uguggauaag ucacucagga aagaaaucgg acugcuuuac     3720

agcaaguaug gucagaaugg caguaaaaac aucauuaagu acuuuaaaga ccgugacaau     3780

aagcauaacg ggcuggaugu uucuaaagug aauguacacu auuucgacaa cgauuacgcu     3840

gcaucccggg ucgccuugga ccauaaguuu aauagcgcua agaucaaauc gauaacagau     3900

acugggauuc agaccauccu ccugaagcac cuagagucau auaacgaaau uaauggcgac     3960

aaaaucauag agcaccccga gcuggccuuc ucugcagaug gucuugacau ucucaacgcg     4020

aauaucacga agcugaacaa ugggaaauuu cauaagccga ucaagaaagu gagaaagacc     4080

gaaacauugg gccugaaauu cauuguggga cagacuggca acaagaggaa aaaguacguc     4140

gaggccgcug augggaccaa cuuauuuuuc gccaucuaug uuagugugga cggaauuagg     4200

uccuacaaga caauaccucu ccgagaagua gcagagagac ugaaagaagg ccuuauccca     4260

gugcccgaga agaaugauaa agacgaucac cuguuguuuu ggcucagccc uggugaccug     4320

gucuacgugc ccucuauuga ggaagagggg cggaucguug aaaucgagaa cauuaagcaa     4380

gacaggauau auaaaguggu cagcugcacu gaaaaugagu gucauugcau cccacacuuc     4440

guggcuuccc cuauuguuaa gaccgaugag cuaggagcca acaauaaggc acagcgcgcc     4500

uggucaggcg aaaugaucaa agagacgugc auuaagauca aaaugaaccg ucugggucag     4560

auagugaaga ucuga                                                      4575


<210>  3440
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3440
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cgugcugaau       60

gcugcagaug acaacuccuu gaaguacaua gaggccgcgg guucuagaau cauucccaug      120

gaugcugcca ucuuaggcga cuucgauagg ggaaauucaa uuagucagac agcagaacgg      180

acucgcaucc gagggaugcg ucaccugaga gagagguauc uacuccggcg cgccagacug      240

cauaggauac uuaaccuguu gggcuuucuc ccuuuccacu acgcucaaga caucgaucgg      300

uaugguaauu uuagcgcaga cauggaacca aaaauugccu ggcgcaagaa ccuggagggg      360

gaacuggagu ucaucuuuau ugaguccuuc aaugaaaugc ucgaggauuu ucgaaagaac      420

cagccggaau uagucgcuga caauaaaaag guucccuacg auugggugau cuauuaccug      480

aggaaaaagg cccuugagaa aaagauaucu aaagaggaau uggcguggau ucugcuaaac      540

uucaaucaga agagaggaua uuaccagcuc cggggcgagg acgaugacga aucgaugaag      600

acggcaaaaa cccgucaaua cuuugacaag cagaucauua aaaacaucau cgauacaaau      660

cagacuuaua agggccagcg cauucugaua guagagcuuc aaaacgggga caaaggaaag      720

aucuucaaaa aggaaauucc ugauuggauc ggucaggaga agaauauaau ugugaccguc      780

gacaucgaua aagacggcaa cgauaaguac gaggaaucag ggagccugag uugcagauuu      840

aaaaucccaa cagacgccga gugggaaucc gaguggaagu ugauuaaaau gaagacccag      900

aaggaucucg acgaaucuaa caaaacugug ggaacauaua ucuacgauag cauucugcag      960

aaucccaccc aaaagguuag gggcaaauua gugcggacga uagagcgaaa guauuacaaa     1020

gcugagcuga agcagauccu cgaaaagcag acugaguucc auuccgaacu ucaagacaaa     1080

gaccuguaua uugaguguuu gaacgaacua uacucaaaua acgauaccca ccgcaaugcc     1140

aucacagaca gagauuuugu cuaccuguuc augaacgaca uccucuucua ucagaggccu     1200

cugaagagua aaaagagccu uauugauaau ugcccauacg agcggaacau auauaucgac     1260

aaagagacug gugaaauuaa guacucuccc aucaagugua uugcaaaauc ccauccucug     1320

uuucaggagu uccgcuugug gaaguuuauc ucaagcauaa gaaucuauca gagggaaagu     1380

gugguagagg uuguggauaa acaacucgac cuguucucuu cccagagcac cucgguccag     1440

aaguuaguga cagaugugga cgucaccgau aaauuucuga cgugcgagaa ugacuacguu     1500

gcucuauucg aauggcucaa caagaaaaag auuaucgauc agaaggaguu ucugaaauau     1560

cguccauucg ggcuuaaggg agacauugac aauuaccggu ggaacuacgu ggaagauaaa     1620

ucuuauccgu guaaugagac ucacgccaug aucuugacau acuuugcgaa ggccggcaua     1680

uccgaagaca uucugaccaa agagauggag gaagcacucu ggcacauccu guauucagua     1740

gaggaucgac aagaaauuac uaaggcucuu agaacauucg ccaguaagua ccaucugagc     1800

gacuccuuug ugaaagucuu cucuaacaua cccccuuuua agaaagagua uggcagcuac     1860

ucagcuaagg ccaucaaaaa guugcucagu cugaugagga uggggaagua uugguccgaa     1920

gaugcaaucg acgugaauac ccgcgagcgg auugauaaaa ucguuacggg agaguacgac     1980

gaaaagauua gagauagggu gcgcgagaaa accauccgau uaaacgacau acggcacuuc     2040

cgugcccugc cagauuggcu agcuugcuac gucguguaug acagacauag cgaaacaagg     2100

gagauuacug aauggaaguc ucccgaggag aucgaugaau acauccgcaa cuucaaacag     2160

cacucacucc ggaauccuau uguagagcag guuaucaccg aaacacugag aacugugagg     2220

gacauuugga agcaggucgg uuguauagac gagauccacg uggagcuugg ccgagaaaug     2280

aagaacccag cggauaaacg caagcggauu accagucaaa ucauugagaa ugaaaacgca     2340

aauuugagag ucaaagcccu gcucacagag uuugugaacc ccgaguucga gaucgagaau     2400

guuaggccgu auagccuggg gcagcaggac auauuacgua ucuacgaaga uggagugcug     2460

aacuccguag cugagguccc uggcgacauu gccgguaucc uuaagaaauu uaaugaaaac     2520

gaugcaaaga aacgccccac cacuucugag auuuugcggu auaagcucug gcuggaacag     2580

aaguacagau cgccauauac gggggagaug aucccucuaa gcaaacuguu cacauccgcc     2640

uacgagauag aacaugugau uccccaauca agguacuuug acgauaguuu cucuaauaag     2700

guuaucugcg aggcugaagu gaacaaacuc aaggacaaug cccugggcua ugaguuuauc     2760

aaaaagcacc auggagaaaa ggucgagcuu ggcuucggga aaauugugga gauauuuagc     2820

guauccgugu acgaacaguu cguuaaggau aacuauucac gaaaucgggg aaaaaugaag     2880

aaauugcuga uggacgagau cccagaacag uuuauugagc gccaauuaaa cgauagcaga     2940

uacaucucua aggucgugaa gacccuccug uccaacgugg ucagggagaa agcagaacag     3000

gaggcgguua guaagaaugu gauugugugu acugguggcg uaaccgaccg gcuuaaaaag     3060

gacuggggga ucaacgaugu cuggaauaaa auagugcugc cccgauucuu gagacucaac     3120

gaacugacaa auagcaccaa cuuuacugcu aagaaugccg caggaaacga gaucccugcc     3180

augccacuag aacugcagaa gggcuucaau aaaaagcgca uugaccacag gcaucacgcu     3240

auggaugcca ucguuauugc augcgcuaac cguaauaucg ugaacuaucu caauaacgag     3300

ucugccuccc ggaaagcgaa gauaucaaga cacgaccugc aggccauucu uugugauaaa     3360

gucaggacag acgauaaggg uaauuaccaa uggcgcguga agaaaccgug ggacacguuc     3420

acccaggaug uuuauguggc uuuggagaac aucaucguaa guuuuaagca gaaccugcga     3480

gucauuaaua aaacuagcaa cuacuacugg cauuaugaaa augggaagaa aguguuagug     3540

aagcagguca aaggcgacuc gugggcaauc agaaagccca ugcacaagga uacaguuuuc     3600

ggagagguga accuccggaa aguaaagucc gugucucuga augaagcccu uaaaaacccu     3660

caauuuauag ucgagaagga ccugaaaagg cagcuacuca gccuguugca gcagaauuuc     3720

gauauuaaga agaucaaaaa guacuuugaa gaacauaaag acauuugguc agacaucagu     3780

cuguccaaga ugaacauaua uuacuucacc aaagagacaa aggaucgcua uuuugcuacu     3840

cguaagccau uagacgccuc uuucgauaaa aagaaaauug aaagcugcau caccgacacg     3900

ggcauccaac agauucuccu gagacaccuu gagcugaagg aaaaugaucc cgagcgggca     3960

uuuuccccug acgggaucga ugaaaugaac aggaauauua uacaguugaa caaaggaaag     4020

ggucaccauc caaucgcgaa ggugcgcguu uacgagaaag ccgacaaguu cgaggugggc     4080

caacgaggga auaaaucaag caaguuuguc gaagcugcca aaggaaccaa ccucuucuuc     4140

gcaguguaug agacagaaua ccccgaugag aagacuaaua guauuguuag aaaacggaac     4200

uacgcuacca ucccgcugaa uaucgccauu gagaggcaga agcgcggccu aucuacagca     4260

ccugaagacg agaaggguaa cgugccaucc uuuguccuga gccccaacga ucuuguauau     4320

cucccuacuu cggaagacau agugaauggg cacaucaccc agcccauuga uaaaucaaga     4380

aucuacaaga ugguuucuag uuccggcagc cagugcuucu uuauuaaaca uaacaucgcc     4440

aauauaaucg uggacaagua ugaguucucu ccacugaaca aaauggaaag ggcuauuagc     4500

aaugagauga ucaaggagau uuguaucccu gucaaggugg accgguuggg aaacauagua     4560

aaacugggca aguccgaaug a                                               4581


<210>  3441
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3441
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cgugcugaau       60

gcugcagaug acaacuccuu gaaguacaua gaggccgcgg guucuagaau cauucccaug      120

gaugcugcca ucuuaggcga cuucgauagg ggaaauucaa uuagucagac agcagaacgg      180

acucgcaucc gagggaugcg ucaccugaga gagagguauc uacuccggcg cgccagacug      240

cauaggauac uuaaccuguu gggcuuucuc ccuuuccacu acgcucaaga caucgaucgg      300

uaugguaauu uuagcgcaga cauggaacca aaaauugccu ggugcaagaa ccuggagggg      360

gaacuggagu ucaucuuuau ugaguccuuc aaugaaaugc ucgaggauuu ucgcaaaaac      420

cagccggaau uagucgcuga caauaagaag guucccuacg auugggugau cuauuaccug      480

cgaaagaagg cccuugagaa aaagauaucu aaagaggaau uggcguggau ucugcuaaac      540

uucaaucaga agagaggaua uuaccagcuc aggggcgagg acgaugacga aucgaugaag      600

acggcaaaaa cccggcaaua cuuugacaag cagaucauua aaaacaucau cgauacaaau      660

cagacuuaua agggccagcg uauucugaua guagagcuuc aaaacgggga caaaggaaag      720

aucuucaaaa aggaaauucc ugauuggauc ggucaggaga agaauauaau ugugaccguc      780

gacaucgaua aagacggcaa cgauaaguac gaggaaucag ggagccugag uugucgcuuu      840

aaaaucccaa cagacgccga gugggaaucc gaguggaagu ugauuaaaau gaagacccag      900

aaggaucucg acgaaucuaa caaaacugug ggaacauaua ucuacgauag cauucugcag      960

aaucccaccc aaaagguuag aggcaaauua gugaggacga uagagcggaa guauuacaaa     1020

gcugagcuga agcagauccu cgaaaagcag acugaguucc auuccgaacu ucaagacaaa     1080

gaccuguaua uugagugcuu gaacgaacua uacucaaaua acgauaccca ccgaaaugcc     1140

aucacagacc gcgauuuugu cuaccuguuc augaacgaca uccucuucua ucagagaccu     1200

cugaagagua aaaagagccu uauugauaau uguccauacg agaggaacau auauaucgac     1260

aaagagacug gugaaauuaa guaccugccc aucaagugca uugcaaaauc ucauccuuug     1320

uuucaggagu uccggcucug gaaguuuauc uccucaauac gcaucuauca gagagaaagc     1380

gugguagagg uuguggauaa acaacuggac uuauucaguu cucaguccac cagcguccag     1440

aagcugguga cagaugugga cgucaccgau aaauuucuaa cgugugagaa ugacuacguu     1500

gcucucuucg aauggcugaa caagaaaaag auuaucgauc agaaggaguu ucuuaaauau     1560

aggccauucg gguugaaggg agacauugac aauuaccguu ggaacuacgu ggaagauaaa     1620

ucguauccgu gcaaugagac ucacgccaug auccugacau acuuugcgaa ggccggcaua     1680

ucugaagaca uucucaccaa agagauggag gaagcacugu ggcacauccu uuauuccgua     1740

gaggaucggc aagaaauuac uaaggcucug cgaacauucg ccucaaagua ccauuugagu     1800

gacagcuuug ugaaagucuu cuccaacaua cccccuuuua agaaagagua uggcucuuac     1860

agcgcuaagg ccaucaaaaa gcuccuguca uuaaugagaa uggggaagua uuggagugaa     1920

gaugcaaucg acgugaauac cagggagcgc auugauaaaa ucguuacggg agaguacgac     1980

gaaaagauuc gggauagagu gagggagaaa accauccgcc ugaacgacau acgacacuuc     2040

cgggcccuac cagauuggcu cgcuuguuac gucguguaug accgucauuc cgaaacaaga     2100

gagauuacug aauggaaaag ccccgaggag aucgaugaau acaucaggaa cuucaaacag     2160

cacucucugc gcaauccuau uguagagcag guuaucaccg aaacacuucg gacugugaga     2220

gacauuugga agcaggucgg uugcauagac gagauccacg uggaguuggg cagggaaaug     2280

aagaacccag cggauaaacg aaagcgcauu accucacaaa ucauugagaa ugaaaacgca     2340

aaucugcggg ucaaagcccu ccugacagag uuugugaacc ccgaguucga gaucgagaau     2400

guuagaccgu auaguuuaag ccagcaggac auacugagga ucuacgaaga uggggugcuu     2460

aacuccguag cugagguccc uggagacauu gccggcaucu ugaagaaauu uaaugaaaac     2520

gaugcaaaga aacgucccac cacuucugag auucuccgcu auaagcugug gcuagaacag     2580

aaguaccggu cgccauauac gggugagaug aucccucuga gcaaacucuu cacauccgcc     2640

uacgagauag aacaugugau uccccaauca agauacuuug acgauaguuu cucuaauaag     2700

guuaucugug aggcugaagu gaacaaacug aaggacaaug cccuugggua ugaguuuauc     2760

aaaaagcacc auggcgaaaa ggucgaguug ggauucggca aaauugugga gauauuuagc     2820

guauccgugu acgaacaguu cguuaaggau aacuauucaa ggaaucgagg gaaaaugaag     2880

aaacuguuaa uggacgagau cccagaacag uuuauugagc ggcaacucaa cgauagccgc     2940

uacaucucua aggucgugaa gacccugcuu uccaacgugg ucagagagaa agcagaacag     3000

gaggcgguua guaagaaugu gauugugugc acuggaggug uaaccgacag gcugaaaaag     3060

gacuggggca ucaacgaugu cuggaauaaa auaguguugc cccgguuccu ccgacugaac     3120

gaacuaacaa auagcaccaa cuuuacugcu aagaaugccg cagggaacga gaucccugcc     3180

augccacugg aacuccagaa gggauucaau aaaaagagaa uugaccaccg ccaucacgcu     3240

auggaugcca ucguuauugc augugcuaac aggaauaucg ugaacuaucu gaauaacgag     3300

ucugccuccc guaaagcgaa gauaucacgg cacgaccuuc aggccauuuu gugcgauaaa     3360

gucagaacag acgauaaggg caauuaccaa uggaggguga agaaaccgug ggacacguuc     3420

acccaggaug uuuauguggc ucuggagaac aucaucguaa guuuuaagca gaacuuacgc     3480

gucauuaaua aaacuagcaa cuacuacugg cauuaugaaa augguaagaa agugcucgug     3540

aagcagguca aaggggacuc gugggcaauc cgaaagccca ugcacaagga uacaguuuuc     3600

ggcgagguga accugagaaa aguaaagucc gugucucuua augaagcccu gaaaaacccu     3660

caauuuauag ucgagaagga ccuaaaacgg cagcuccuga gcuugcugca gcagaauuuc     3720

gauauuaaga agaucaaaaa guacuuugaa gaacauaaag acauuugguc agacaucagu     3780

uuauccaaga ugaacauaua uuacuucacc aaagagacaa aggauaggua uuuugcuacu     3840

cgcaagccac ucgacgccuc uuucgauaaa aagaaaauug aaagcugcau caccgacacg     3900

ggaauccaac agauucugcu ucgucaccug gaguugaagg aaaaugaucc cgagagagca     3960

uuuuccccug acggcaucga ugaaaugaac cggaauauua uacagcucaa caaagggaag     4020

ggacaccauc caaucgcgaa ggugaggguu uacgagaaag ccgacaaguu cgaggugggu     4080

caacgcggca auaaaucaag caaguuuguc gaagcugcca aagggaccaa ccuguucuuc     4140

gcaguguaug agacagaaua ccccgaugag aagacuaaua guauuguucg aaaaagaaac     4200

uacgcuacca ucccgcuaaa uaucgccauu gagcggcaga agaggggacu gucuacagca     4260

ccugaagacg agaagggcaa cgugccaucc uuuguccuua gccccaacga ucucguauau     4320

cugccuacuu cggaagacau agugaauggu cacaucaccc agcccauuga uaaaucacgc     4380

aucuacaaga ugguuucuag uuccgggagc caguguuucu uuauuaaaca uaacaucgcc     4440

aauauaaucg uggacaagua ugaguucucu ccauugaaca aaauggaaag agcuauuagc     4500

aaugagauga ucaaggagau uugcaucccu gucaaggugg acaggcuggg caacauagua     4560

aaauuaggaa aguccgaaug a                                               4581


<210>  3442
<211>  4584
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3442
auggccaaga uccugggccu cgaccuuggg accaacagca uuggaugggc uauaguggag       60

aaagauggua augacuucuc ccuggucgau aagggcguua gaaucuuuuc ugaaggggug      120

aaaucagaga agggaauuga aaguagcagg gcagccgagc ggacaggcua ccgcuccgcg      180

cgaaaaauca aguaucguag aaaguugagg aaauacgaaa cuuuaaaggu acugucucua      240

aacgguaugu gcccccucuc gauugaggag guggaagagu ggaaaaaguc aggguucaaa      300

gaauauccuc ugaauccaga guuucuugag uggcugcgga cgagcgacga ugaaaacauc      360

aauccguacu ucuuucgcga caaggcuagu aagcagaaag ucaccuuguu cgaggugggc      420

agagccuuuu aucacauagc acaaaggcgg ggauuccucu ccaaccgccu ggaucagucu      480

gccgaaggca uccuggagga acauuguccc aagauugagg cucucguuga ggacuuaaau      540

agcacagaug aaauccugac uucccuuaaa gacuacuuuu ucgauaccgg gauuuuggac      600

gcaaacgaga agggagguuu ugccaaagau cuggacgaag gcgacaagaa acucguguca      660

cucuaucgaa guauccaggc uauagucaag aagaaugaua cagacuucga gaaagggaag      720

gccgaaauua ucgagagacu gaacaaaaag gaugugcuug gaaaaguaaa gggcaagauu      780

aaagacauca gccaggcgau gcuggagggu aauuuuaaga ccuuggggca auacuucuuc      840

ucucucuaca acaaagaaag gauccggaau caguauacuu cccgugagga ucacuaccug      900

gaagaguuua agauuauaug caaauuccag ggaaucggcu caauuaacca gaaugaaaag      960

uuaccugaga agaaauuuga gggccuggca aaggaacucu auaaagccau cuucuuucaa     1020

cgcccacuua agagccagaa agggcugaua ggaaagugua guuucgagaa gucuaaaucc     1080

agaugcgcua uuagccaucc cgacuuugaa gagaagagga ugugggagua cuugaacaca     1140

aucaaaaucg guacccaguc ggaaaagguu cuacgguucc ugucucagga ggaaaaacuc     1200

aaccugauuc cuaaguuucu ucgaaagaag gauaauuuca acuuugagga ccuggccaag     1260

gaauugaucg agaaaggcgc aacguucggg uuuuauaagu ccucaaaacg cgauaguuuc     1320

cacuacuggu ucaauuauaa gccaacugac accgugagcg gaugucccgu cuccgcuucu     1380

cucaaaagcg uggugggcga ggauuggaag acaaagucau uuauuuacaa aacuaccaac     1440

aguaagggua uagaagucuc cagaacaguu aauuacaaag accuguggca uuuacugagc     1500

gugucuaccu cagacaucua ucuauacgag uaugccaaga acaaacucgg gcuggaagau     1560

aagaaugcga aggccuucag uaaaauuaaa cuuaaaaagg acuuuggcaa cuugagccug     1620

aaugcaauca acaaaauccu cccuuacaug agggagggac ugcuuuauuc ccacgcugua     1680

uucauggcca auauugaauc uauaguggau gaggucaucu ggaacgaccu gggccaacgg     1740

aaguacauuc aggauaagau cgcugccauu aucgcaaaua acauagugga caauggguug     1800

cucgagguug ugaacggacu gaucaaagaa ugcaaggucg auggugccua uuacucgaaa     1860

gaggcugcgg acacguaccg caaggauuua agcaaaaagc ugguguccuu uuucaaauuu     1920

caccauauug acgauggcau caagcaggaa acuauucuaa acgagcuauu cccaguauuu     1980

aucgaacaac ugaaggcaca cgaguucaua aaaguuaaga gacuugacga gaaaguguug     2040

gaauuucuga agggggagaa ucaggaugga gaagucuucu guaacaaugc caaacagcuc     2100

aagaagcugu aucauccguc agacauugag guguuuaaaa agcagaucgu caaagacaag     2160

gauggcacag agaccgguca auuaauucug gggagucccc uuacuucuag caucaaaaac     2220

ccuauggcua ugagggccuu gcaccagcuc cguaaggugc ugaauacacu aauaguugaa     2280

gaacaggugg acuccucaac ccggauccac auugagaugg cacgagagcu gaacgaugcc     2340

aauaagagaa aaggcaucca ggacuaccaa aacgaaaaua agaaagagag ggagaaguau     2400

cgcgaugaaa uuaaaaagcu cuaccuggcu gagugcaaca aggaaaucga gccaacggua     2460

gacgaucuuu ugcgguauca gcuguggaua gaacaggaca gaaaagagau uuacgagaau     2520

ggaaacagca ucucuaucug ugauauuauc ggcgacaauc ccgccuauga uauugaacau     2580

accgucccua gguccauaag ucaggacaac agccaaauga auaagacauu augcucucag     2640

aaauucaacc gcgaugugaa gaaacagaag augccauucg agcucgggaa ccaccuggaa     2700

auccuucccc gaauugcaca uuggaaggag gaagcggaca aucugucccg ggagaucgag     2760

augauuaucc guucaacuaa aaccgcugcc acaaaggaag caaaagacaa gaaaauaaga     2820

aggcgccacu acuugacucu ccggagagau uaccugcagg gaaaguauga cagguuuauc     2880

uggaccgagc cgaaaguugg uuucaagaac agucaaauuc cugauacagg caucauuacc     2940

cgauacgccc aggcuuaucu aaagagcuac uuuaaucgcg uggaaucggu caaaggggga     3000

augguggccg aguuccggaa gaucuggggc auacaggagu ccuaucaaga aaacgguaaa     3060

aaguacuuua aagagaagga cagaucuaag cauacucacc acacgauuga ugcaaucaca     3120

aucgcuugua ugaccaaaga caaguaugau guacuggccc augcguggac ucucgaagac     3180

aaagagcaga uuaccgaagc caggaagaua guggcugaga gcaaacccug gaagacauuc     3240

aaggaggauc ugaaaaagau cgaagagggg auucuuguuu cacacuacac cccagacaau     3300

gucaaaaagc agaguaaaaa gaucgugcgu gugcgcggcc ggaagcagua cgucgcagaa     3360

auugagagag auguuaacgg aaaagccgug gcuaagaaag acaucuccgg caaggugaua     3420

uauaaauugg acaaggaugg gaaaaagcug ccuagguuac aacagggaga cacuauccga     3480

ggcucucucc aucaggauag cauuuacggu gccaucaaga auccccugaa aacagaagag     3540

auucgguaug uaauccgcaa ggaccuugag uccauaaaag caucagaugu cgacaacauu     3600

guggaugaaa ugguuaagga gaaagugaag gaagcgaucg ccagcaaggu ccugauccua     3660

aguucuaauc cacagcagaa aaacaagauu gacgggaaag uguggaugaa ugaggaaaag     3720

ggaaucccca uaaacaaagu uagacucuac gcuaauuccg ugaagaaccc ucuggagauu     3780

aaggagcaca gcucguuguc aaaaucuagg cacgaacaua agcaaaaagu auauggccag     3840

aaugaugaga acuuugccau ggcaaucuac gaaggucugg acaagaaagg caagacggag     3900

cgggcuuucg aguuaauuaa cuauauggau gccggguacu acuauaagcu caaaaauaag     3960

aacgaacgaa agaaggacau cauaagucug gucccagaga aacacauuga aaccggauuu     4020

aaucuucuga gauccaacaa gaucccgauc auuuugaaaa agggccucaa ugugauccug     4080

uacaagaacg agaaagaaga gauugauuuc aauaacccca auagcauaau caagcgccua     4140

uauaaaauuu cugggcugga caaggacgga aucaaacuua uguacugcau cagcgcaaag     4200

ggcuccacug agaacauugc cuauaugaau gaugugauaa acaagcagaa acucuuugaa     4260

cugaagucag agauggaaaa auugggucug ugggacaagg ucuucaguga uuucaaaaau     4320

gacaacuuaa agaaaagcga cuuuuucaaa aagcucaaug aucugcuuaa caaacauuac     4380

uuugagaaua acguuugcga uucuaucggg uccauuaaga aagugaagau caaggaauca     4440

ggacugacaa ccccuaaagg cggugacgua auugauaacu ucaaggacuu uccauauauc     4500

aaauucaagg ugaguaauuu uaacgcuuug auagagggca ucgauuucaa aauuagcccc     4560

acagggaagu uuguccaaau cuga                                            4584


<210>  3443
<211>  4587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3443
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugguggag       60

caggauuuca agaauaaaca agguaacaua augggcaugg gguccagagu cauccccaug      120

ucucaggaaa uuuugggaga guuugcuagg ggcaauacaa agucagaaac ugcagagcgg      180

acgcgcuuac gaaguguucg uagacugagg gaacggcacc uacuccgcag agagaggcug      240

caucgggugc uucacauccu ggguuucuug ccuaaacauu acguaaacca gauugacuuu      300

caggagcgcc ucgggaaguu cuuugaagag agcgaaccaa agcuggccua ugauaccgga      360

gacuuccugu uuaaaccguc cuuccucgag auguuagagg aauuuaagau ccaccaaccc      420

ucuuucgugg agggcgauaa aaaggucccu uacgacugga caauauauuu ucugcggaaa      480

aaggcgcuua cugaaagaau cgagaaggaa gaguuggcuu gggugauucu gucguucaau      540

cagaaaaggg gcuacuauca gcuacgaggg gaggaugaag aggaaaagcc aaacaaacuc      600

gaggaauuuu acucacugaa gaucguugac auuaaagugg augagagccc caauaagaaa      660

ggagacaccu gguauagucu uacacuggag aacgguugga ucuaccgucg cuccucuaag      720

gucccuuugg augacuggcu cggcaagacc agagauuuca uagugacuac agacauugac      780

gaagagggga auccaaaacu ggauaaggaa ggaaacccca aaaggagcuu ccgggccccu      840

uccgaggacg auuggggcuu acgaaagaaa aagaccgaaa aggagauccu ggacucaagu      900

aaaacgguag gucauuuuau uuucgagaau cuccuugcag auccaacuca gaagaucaaa      960

gggcgccugg uuagaaccau cgaaaggaag uuuuacaaag acgaguugaa gcaaauucua     1020

gugagccaga accgguucca cccggaacug ucugaugccg acaugauggc ugauugcguc     1080

cgcgagcucu auaagcacaa ucauucccac cagaacuuuc uggcaucaaa agacauaaag     1140

caucuucugg uggaugacau cauuuucuac cagagacccu ugaggagcca aaaaucaaca     1200

aucggaaauu guccucucga guuuaagacu uucaaaaagg acggcauaga aauuaaggug     1260

ccauauaaag ucgcccccaa gucucacccu cuguuucagg aguuccguuu acugcagugg     1320

guuuccagcc uacggaucau ccgaaaaucg gaugacucug augugaccag agaauuucuc     1380

ggcacacaag acaagaccaa acagcugcuu gauuacuuga ugacgcaguc cgagauggac     1440

ugcaacaagc ugaugacuuu ccuccugaca caggaagggu auaaaaggaa ggaucuuaag     1500

ucagagguag gaaaauuucg cuggaacuac guguucgaca gugagaccgg ugaaagcaag     1560

acuuauccag gcaauuccac acgggcugag uuccugaaaa gauuggggaa ggucgaagga     1620

uuugauuucu cuaaauacac cgaggaauuu gaggagagcc ucuggcaccu gguguacuca     1680

guuacggaca agguggaauu ugagaaggcc uuaaggaaau uuagucgcaa gaacggcauu     1740

gaaaucaauu ccuucgucga gaaccugaaa aaguuucccc cguauaaaag cgaauacgcg     1800

ucuuauucac uaaaggcaau uaagaaacuc cuggcccuua ugcgauucgg ugagaauugg     1860

aaggaggaua guauagacag caucuccaaa caucggauug aaaagaucuu gaccggggag     1920

uacgauccug aaaucaaaga caaggugcgu gagauuucua gagcuuuuga cgagguagaa     1980

gauuuccaag gccugcccgu uugguuugcc aacuaugugg ucuacggaag gcacucggag     2040

gcaagcgaac ucauaaagug gacauccuca gacgagaugg cugauuucau caaagaauuu     2100

aagcagcaua gucugcgcaa uccaauugug gagcaggucg uguuagagac ucugcggguu     2160

gugaaagaca ucugggauaa guauggccac gggcagaaag acuucuucga ugaaauucau     2220

guagagcuug gaagagaauu gaagaacccu gccgagcaaa gggcgcgacu caccacacag     2280

aucucugcca augaaaacac uaaucugcgc auaaaaagcc uacuggucga gcuccugcac     2340

gaccccggug uggagaacgu ucggccauac ucccccaugc agcaggaaau ccuuaagauu     2400

uacgaggaag gcguguugaa gaguagcuuu gaggucccug augacauccu gaaaauuucu     2460

aagaccgcac aaccauccag uagcgauuua aaaagauaua agcucuggcu ggaacagaaa     2520

uacaggucuc cguauacagg gaagaucaua ccccuuaaua agcuguucac cccugcuuac     2580

gagguggagc acguaauucc acagucccgu uauuuugacg auucauucag uaacaaagug     2640

aucugugaag ccgcuguuaa uaaguugaaa gacaaccgcc ucggaaugga guuuauuaag     2700

ugcuuccaug gccagcgggu cagccugggu uuuggggaag agguggagau cuucucggaa     2760

uccgccuaca aagcauuuau acaagacaac uuccccaaga auccugccaa gaaaagaaag     2820

cuacugaugg augagcuccc agaacagaug guggagaggc agaugaacga cacucgauau     2880

aucucuaaau uugucacgag cauucuguca aauaucguuc ggagugaaga gaacgauaca     2940

ggcuucaauu ccaagaacau uauccccgga aauggcaaaa uaaccacugc ucuuaagcaa     3000

gacugggggu ugaacgaugu guggaaugag cugauuuuac cgcgcuucga aagaaugaac     3060

cagcucaccg gaacaaccgu guuuacugcg uggaaugaga accaccagaa guuccugccu     3120

acaguaccac uugaauacgc aaaagguuuu cagaagaaaa ggaucgacca ucggcaccac     3180

gccauggaug cucuggucau cgccugugca acgcgagacc augugaauua ccuaaacaau     3240

gagcacgccc ucggcaagaa cuauaaaggg aaggaagaga agcaaucuag acgcuucgau     3300

cugaggcaga aauugugccu gaagaaaaag aacggacaua gccaggagca guacgaaugg     3360

auuuucucca aacccuggga cucauucacc caagaugcua agaaaaauuu agagcguauc     3420

auuguuagcu uuaagcagaa ccuccgggug auaaauaaga caaguaacaa auaucugucu     3480

uacaaggacg aagagggcaa ucuuagacug gacucccagg guaaaccugu caaggccuug     3540

acaaggcaga ucaaagggga ugcaugggcg auucgcaaga gcauccacaa ggacaccgug     3600

ucgggccucg uuaaacugcg aagagugaag augguaaacc uaucagucgc ucuggauaau     3660

cccgaaaaca uucuuaauaa aaagcucaaa aagaagaucg ccgagcuggu gaaauugaac     3720

uaugacaaua agcuguuacu caaauacuuc cgggauaggg acuauauguu ugauggacgc     3780

gaggugucua agcuggacgu cuacuacuuc gaagagggcc uugcagccag ucguguuucc     3840

cuggauacua gcuuuucuga caaaaagaua caaagcauca cugauaccgg gauuagaaag     3900

aucuugcucg cucaucuggc caacuaucac ggacggcuag acgaaaaagg caaggugauu     3960

ccaccugaga cgcuggcauu cacccccgag gguaucgagg agcuuaauaa aaacauaaag     4020

gggcucaaug gaggcaaauu ucaccagcca auuuucaagg uaaggacaua cgaaccgaaa     4080

gguaacaagu uccagguggg cuuuaagggg aacaaaaauu ccaaguaugu cgaggcugcc     4140

aaaggaacua accuguucuu ugcgaucuac uugaaugaug aaggcaagcg cucauaugag     4200

accaucccuu ucaacguggu uauggagcga uuaaaacaag gggaaggacc cgugccacag     4260

acaaaugaga agggcgguaa gcugcucuuu caucugaguc ccaacgaacu ugucuacgug     4320

ccugagacug acgaaaauua uaccaacguu gacuucaaaa aucugagcaa ggaacaggug     4380

gagaaaauuu acaagauggu cucuuccacg gggucagaau gucacuuugu aagagccgau     4440

auaaguagcu ugaucaaagg auacgacgcu aagacaaagg ugggcgagcu agguucccug     4500

aacaaacagg aaguuaccaa uaacggcaug acucggauua aggagaaaug cuggaagauc     4560

aaaauugaua ggcucggguc uaucuga                                         4587


<210>  3444
<211>  4587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3444
augaagaaaa uccugggcau ugaccucggg accaacagca uaggaugggc cguggucaau       60

uccuucgaug agaaccagaa ugaagagucu cuuaagcaca ucgaaugcgu ugguucaaga      120

auuaucccca uggacgcugc aauucugggc gauuuugaca aagggaacag uaucagccaa      180

acagccaaga ggacugaguu ccgguccgug cgccauuugc gagaacguca cuuacugaga      240

agggcgcggc uacauaaaau acucucucug cuuggauuuc ugccugauca cuacguacag      300

gacuuggauu cgcauggcaa uuucccagug aacgucgagc cgaagaucgc uuggcgcaag      360

gacucaaaug gugccuuuga guucauuuuu aacgauagcu ucgacgaaau gcuccaggau      420

uuugcaacgu aucagcccga gcugaucgcc uguaauaaaa aggugccuua cgacuggacc      480

auuuauuacc ugagaaagaa ggcucucacu aaaaaguuaa guaaggagga gcuguccugg      540

auccuuuuga acuucaauca gaaaaggggg uauuaccaac ugcggggaga ggaagaugac      600

gagucuguua agagcgcaaa aacacgccag uauuuugacc gacagaagau aaaauccauu      660

guggauacca accaaacaua caagggccag aaaguccuaa ucguggaacu cgaggacggc      720

auuaagggga agaucuucaa aaaggaaauc ccaaauuggg uaggacagga gaaaucaauu      780

auaguuacca ucgauauuga caaggauggu ugcgacaaau acgaggaaag uggcgagcug      840

agcuguagau uuaagauccc cucugaugcc gaaugggagu ccgaauggaa acuuauaaaa      900

augaagacuc agaaagaccu ggaugcuuca gggaagacag ugggagccua uauuuacgac      960

gcgaucuugc aaaacccuag ccagaaaauc aggggcaagc ucguccggac cauugagcgu     1020

aaguauuaca aagaggaacu gaagcagauc cugaaaaagc aaguggaguu ccacccagaa     1080

uuacgcgaua aagaccucuu ccuugacugc cugaaugagu uguaucccaa caacgaugca     1140

cacagaaaug ccugugcuga cagggauuuu auuuaccuau ucauggacga uauacuguuu     1200

uaucagcggc cucucaagag uaagaaaucu cugaucgccg acugcccaua cgaaaagcau     1260

auguaccgag auaaagagac gggugagcuu aaguauucca gcgugaaaug uauugcaaag     1320

ucgcacccgc uguuccagga auuucgcuug uggcauuuca ucucuaacau cagaauuuac     1380

cagaaagaga gggucguuga cguggaugcu gaccaauccg cccucucaag ugcgcggagc     1440

gcccugaagu ccaaguuaga agcagaugua gacgugacua augaguuucu gaccugcgag     1500

gcugauuaug ucucucuauu cgaauggcuc aacaauaaaa agagcguguc acagaaagac     1560

uuucugaagu accccgccuu cgggaucaaa aagaguggag acaacuaucg cuggaauuac     1620

guugaggaua aguccuaucc uuguaacgaa acaagagcuc uuauauugag ccaccuggag     1680

aaagccaaua uuaggggcga caucuuuacu cgugaacagg aggaggcacu cuggcacauu     1740

cuguacucug uggaagaucg guacgagauc gucaaggccc uucagucauu cgcugcgaaa     1800

uaucgauuua acgacgauuu cgugaccgua uucaguaagu uuccacccuu caaaaaggaa     1860

uacggcagcu auuccgcaaa ggccaucaaa aagcuguugc cucucaugag aauggggaaa     1920

uacuggucug aggcugccau agacauuaag acaagggaac gcaucgagaa aauuaucacc     1980

ggagagugcg augacucgau acgggaaaga guuaggcuga aggcaauucg cuuacaugau     2040

aucggugacu uucgagcccu gccagugugg cuagcuuguu augucgugua cgaucggcac     2100

agcgaguccg gcgacauuac gaaguggaaa acuccccaag aaauggauug guauauccag     2160

aauuucaagc agcauucacu ccguaacccg auagucgaac aggugaucac agaaacccug     2220

agaguuguga gggacauuug gaaacaagua gggcgcaucg acgagauuca cgucgagcuu     2280

ggacgggaaa ugaagaaucc ugccgauaaa agagcaaaga ugacugcgag gaugaucgag     2340

aacgaaaaua caaacuugcg aauaaaagcu augcugaccg aguuucucaa ccccgaguuc     2400

gaaauugaga augugcgccc auacagugcc ucucagcagg acauccugcg gaucuacgaa     2460

aacggcguuu uaaauagcgu guccgagauu ccugaagaga ucacggguau ucugaagaag     2520

uuuaacgaga gagaacagag gaaacguccc ucaaccagcg agguccuucg cuauaaguug     2580

uggcucgaac aaaaauaccg gucuccauau acagggaaga ugauaccccu gggcaaacua     2640

uucacuccug cauacgagau cgaacauauu aucccacagu ccagauauuu ugaugacagu     2700

uucaauaaca aggugauuug cgaggccgag guaaauaagc ugaaaggaaa ccuccugggc     2760

uacgaauuua ucaagaaaca ccacgggagg cgaguggauc uuggauuggg ugagauaguu     2820

gacaucuuca gcgucgauga auaugagacc uuugugaagg acuacuacuc ugcuaaucag     2880

cagaaaauga agaagcuguu aauggaugaa auuccggagc aauucaucga gcggcagcuc     2940

aacgacuccc gcuauauuuc aaaagugguc aagagucugc uuagcaauau cguuagagaa     3000

caggaugagc aagaaucgau auccaaaaac cugauugugu gcacaggcgg ggugacugac     3060

agguugaaga aagacugggg aaucaaugau guauggaaca agcucauccu gccccgguuc     3120

cagcgacuaa auaacauuac cgagacaacc gaguuuacug ccaagaacau gcagggccau     3180

gaaauaccug ucgugccauu cgaguaccag agagguuuua auaaaaagcg caucgaccac     3240

aggcaucacg caauggaugc uauuguuauc gccugugcgg ggcguaacau ugugaauuau     3300

cugaacaaug aaucugcccg gaaaaaggcu caaaucagca gauacgaccu ccagaggcug     3360

cuuugcgaua aagucaagac ggacgauaac ggcaauuaua aguggauauu gaaaaagccc     3420

uggucaacac ugacccagga cguguacgca gccuuagaua acguuaucgu gaguuucaaa     3480

cagaaucucc gcguaauuaa caagacuaau aaccacuaug uccauuacga ggcuggcaaa     3540

aagaucauug ccaaacaaga aaaggcaucc ugggcgaucc gaaagccuau gcacaaagac     3600

accguguuug gagaggugaa ucugagaaag gucaaaacag uuucucuuaa cgaggccaua     3660

aagagcccac auuguauugu gcagaaagau uucaagaaga aacugcuauc ccuccugaag     3720

cagaauuuug acgcuaaaga aguaaaggcc uacuucgagg aacacaaaga ugugugguca     3780

gacaucaacu ugagcaagau cgaggucuau uacuuuacca aggacacuaa agaacgguuc     3840

uuugcaacaa ggaagccccu ggauaguucu uucgaccgca aaaagauuga guccgugacg     3900

gauaccggga uccagaaaau auuacuccgu caucuggagc aaaagaacgg agacagcgau     3960

cuugcuuucu cgccggacgg uauugaagag augaaucaga acaucguuca gcugaauggc     4020

gggagaaagc accaaccuau ugugaaaguc cggguguaug aaaaggccga uaaauuugca     4080

aucggacagc agggcaacaa ggccucaaaa uacguugagg cugcgaaggg uacuaauuug     4140

uucuuugccg uguaugaaga ggaggucagg gacaaaaaga caaacgcagu aauguucaag     4200

cgcaauuacg cuucuauacc acucaacgug gccauugauc gacagaaaaa ggggcugagu     4260

accgcccccg aagacgagaa uggcaacgcu ccuaucuuug uucuaucccc caaugaucug     4320

guguaucuuc caacagcaga agacaucauu aacgccagaa ucagcuuccc uauuaauaaa     4380

aaccggauau acaagauggu cucuugcacu ggauaccaau gcuuuuucau caaacaccau     4440

guggcgaaug uaauugugga uaagaaggag uuuagcgcuc ucaacaaaau ggagaaggcc     4500

gaaaccaaug agaugaucaa agacaucugu guccccauua agcuggacag guugggcaac     4560

auaguuaaaa ucggggugua uaaguga                                         4587


<210>  3445
<211>  4587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3445
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cguggucaau       60

uccuucgaug agaaccagaa ugaagagucu cugaagcaca uagaaugcgu ugguucaaga      120

aucauuccca uggacgcugc aaucuugggc gauuuugaca aagggaacag uauuagccaa      180

acagccaaga ggacugaguu ccgguccgug cgccauuuac gagaacguca ucugcuaaga      240

agggcgcggc uccacaaaau ccugucucuu cugggauuuu ugccugauca cuacguacag      300

gaccucgauu cgcauggcaa uuucccagug aacgucgagc cgaagauagc uuggcgcaag      360

gacucaaaug gugccuuuga guucaucuuu aacgauagcu ucgacgaaau gcugcaggau      420

uuugcaacgu aucagcccga gcugauugcc uguaauaaaa aggugccuua cgacuggacc      480

aucuauuacc ucaggaaaaa ggcuuuaacu aaaaagcuga guaaggagga gcuuuccugg      540

auuuugcuga acuucaauca gaaaagaggg uauuaccaac uacggggaga ggaagaugac      600

gagucuguua agagcgcaaa aacacgccag uauuuugacc gacagaagau caaauccaua      660

guggauacca accaaacaua caagggccag aaaguccuca uuguggaacu ggaggacggc      720

aucaagggga agauuuucaa aaaggaaauc ccaaauuggg uaggacagga gaaaucaauc      780

auuguuacca uagauaucga caaggauggu ugcgacaaau acgaggaaag uggcgagcuu      840

agcuguagau uuaagauucc cucugaugcc gaaugggagu ccgaauggaa gcugaucaaa      900

augaagacuc agaaagacuu ggaugcuuca gggaagacag ugggagccua uauauacgac      960

gcgauucucc aaaacccuag ccagaaaauc aggggcaagc ugguccggac caucgagcgu     1020

aaguauuaca aagaggaauu aaagcagauu cugaaaaagc aaguggaguu ccacccagaa     1080

cuccgcgaua aagaccuuuu ccuggacugc uugaaugagc uauaucccaa caacgaugca     1140

cacagaaaug ccugugcuga cagggauuuu aucuaccugu ucauggacga uauucucuuu     1200

uaucagcggc cucugaagag uaagaaaucu cuuauagccg acugcccaua cgaaaagcau     1260

auguaccgag auaaagagac gggugagcug aaguauucca gcgugaaaug uaucgcaaag     1320

ucgcacccgu uguuccagga auuucgccuc uggcauuuca uuucuaacau cagaaucuac     1380

cagaaagaga gggucguuga cguggaugcu ugccaacccg accuguccuc agcccggagu     1440

gcguuaaaga gcaagcugga agccgaugua gacgugacua augaguuucu aaccugugag     1500

gcagauuaug ucucccucuu cgaauggcug aacaauaaaa agucugugag ccagaaagac     1560

uuucuuaagu acccugcuuu cgggauuaaa aagucaggag acaacuaucg cuggaauuac     1620

guugaggaua agaguuaucc augcaacgaa acaagagccu ugauacuguc ccaccucgag     1680

aaagcuaaua ucaggggcga cauuuuuacu cgugaacagg aggaggcccu guggcacauc     1740

cuuuacagcg uggaagaucg guacgagauu gucaaggcac ugcagucuuu cgccgcuaaa     1800

uaucgauuua acgacgauuu cgugaccgua uucucaaagu uucccccuuu caaaaaggaa     1860

uacggcaguu auagcgcgaa ggcaaucaaa aaguugcucc cacugaugag aauggggaaa     1920

uacugguccg aggccgcuau agacaucaag acaagggaac gcauugagaa aaucauuacc     1980

ggagagugug augacucuau ccgggaaaga guuagguuaa aggccauacg ccugcaugau     2040

auuggugacu uucgagcacu acccgugugg cucgccugcu augucgugua cgaucggcac     2100

ucggagagcg gcgacaucac gaaguggaaa acuccgcaag aaauggauug guauauucag     2160

aauuucaagc agcauucccu gcguaacccu aucgucgaac aggugauaac agaaacccuu     2220

agaguuguga gggacaucug gaaacaagua gggcgcauug acgagaucca cgucgaguug     2280

ggacgggaaa ugaagaaucc cgcugauaaa agagccaaga ugacugcaag gaugauugag     2340

aacgaaaaua caaaccugcg aaucaaagcg augcucaccg aguuucugaa cccagaguuc     2400

aagauagaaa augugcgccc uuacucagcu agucagcagg acauuuuacg gaucuacgag     2460

aacggcguuc uuaauucugu gagcgaaauc cccgaggaaa uuacggguau ccugaagaaa     2520

uuuaacgaga gagagcagag gaagcgucca uccaccucag aagucuugcg cuauaaacuc     2580

uggcuggagc aaaaguaccg gagccccuau acagggaaaa ugauuccucu aggcaagcug     2640

uucacuccag ccuacgaaau agagcauauc auuccgcagu cuagauauuu ugaugacucc     2700

uucaauaaca aggugaucug cgaagcagag guaaauaaac ucaagggaaa ccugcuuggc     2760

uacgaguuua uuaaaaagca ccacgggagg cgaguggauu ugggacuggg ugaaaucguu     2820

gacauauuca gugucgauga guaugaaacc uuugugaaag acuacuacag cgccaaucag     2880

caaaagauga agaaauuacu cauggaugag auccccgaac aguucauuga gcggcagcug     2940

aacgacucuc gcuauaucuc caaggugguc aaaucacuuc ugaguaauau uguuagagag     3000

caggaugaac aagagagcau cucgaagaac uugauagugu guacaggcgg ggugacugac     3060

aggcucaaaa aggacugggg aauuaaugau guauggaaca agcugauccu accucgguuc     3120

cagcgacuga auaacaucac cgaaacaacc gaguuuacug cuaaaaacau gcagggccau     3180

gagauuccag ucgugcccuu cgaauaccag agagguuuua auaagaaacg cauagaccac     3240

aggcaucacg ccauggaugc aaucguuauu gcuugcgccg ggcguaacau cgugaauuau     3300

cucaacaaug aguccgcgcg gaagaaagcc caaauuucua gauacgaccu gcagaggcuu     3360

uugugugaua aggucaagac ggacgauaac ggcaauuaua aauggauccu gaagaaaccu     3420

uggagcacau uaacccagga cguguacgcu gcacucgaua acguuauagu gucauucaag     3480

cagaaucugc gcguaaucaa caaaacuaau aaccacuaug uccauuacga agccggaaag     3540

aaaauuaucg cuaagcaaga gaaggccagu ugggcaauuc gaaaaccaau gcacaaggac     3600

accguguuug gcgaagugaa ucuuagaaaa gucaagacag uuucccugaa cgaggcgauc     3660

aaaucucccc auugcauagu gcagaaggau uucaagaaaa agcuacucag ccuguugaaa     3720

cagaauuuug acgccaagga gguaaaggcu uacuucgagg aacacaaaga uguguggucc     3780

gacauuaacc ugucaaagau cgaagucuau uacuuuacca aagacacuaa ggagcgguuc     3840

uuugccacaa ggaaaccguu agauagcagu uucgaccgca agaaaaucga aucugugacg     3900

gauaccggga uucaaaagau ccuccugcgu caucuugagc agaagaacgg agacuccgau     3960

cuggcauuca gcccugacgg uauagaggaa augaaucaga acauuguuca guugaauggc     4020

gggagaaaac accaaccaau cgugaagguc cggguguaug agaaagcuga uaaguuugcc     4080

auuggacagc agggcaacaa agcaucgaag uacguugaag ccgcuaaagg uacuaaucuc     4140

uucuuugcgg uguaugagga agaggucagg gacaagaaga caaacgccgu aauguucaaa     4200

cgcaauuacg caucaauccc ccugaacgug gcuauagauc gacagaagaa agggcuaucu     4260

accgccccug aggacgaaaa uggcaacgcc cccauuuuug uucugagucc aaaugaucuu     4320

guguaucucc cuacagcuga ggacaucauc aacgcaagaa uuuccuuccc caucaauaag     4380

aaccggauuu acaaaauggu cagcuguacu ggauaccaau gcuuuuucau aaagcaccau     4440

guggccaaug uaaucgugga uaagaaagaa uuuucugcgc ugaacaagau ggagaaagcu     4500

gagaccaaug aaaugauuaa ggacaucugu gucccaauca aauuggacag gcugggcaac     4560

auuguuaaga uaggggugua uaaguga                                         4587


<210>  3446
<211>  4590
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3446
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc cguggucaau       60

uccuucgaug agaaccagaa ugaagagucu cugaagcaca uagaaugcuu ggguucaaga      120

aucauuccca uggacgcugc aaucuuaggc gauuuugaca aagggaacag uauuagccaa      180

acagccaaga ggacugaguu ccggggaguu cgccaucugc gagaacguca ccuacucaga      240

agggcgcggc ugcauaaaau ccuucgccug uugggcuuuc ucccugauca cuacgugcag      300

gaccuggaug gucaugggaa uuuccuggua aacguggagc caaagauagc uuggagaaag      360

gacuccaaug gaacguuuga guucaucuuu aacgauucuu ucgacgaaau gcuccaggau      420

uuugccaccu aucagccgga guuaauugca uguaauagga aaguccccua cgacuggaca      480

aucuauuacc ugcggaagaa agcccuuacu aagaaauugu cgaaggagga acugucaugg      540

auucuacuca acuucaauca aaagcgcggc uauuaccagc ugcgaggcga ggaagaugac      600

gagagcguga aaaccgcuaa gacaagacag uauuuugaca ggcagaaaau caagaguaua      660

guugauacca accaaacuua caaagggcag aaggugcuua uugucgaacu ggaggacgga      720

gugaaaggua agaucuucaa gaaagaaauu ccuaauuggg uaggccagga gaaguccauc      780

aucguuacag uggauauuga caaagauggg ugcgacaagu acgaggaauc uggagaguug      840

agcugucggu uuaaaauacc auccgaugca gaaugggagu cagaauggaa gcucaucaag      900

augaaaaccc agaaggaccu ggaugccagu ggcaaaacgg ucggugcuua uauuuacgac      960

gccaucuuac aaaaccccag ccagaagaua cgugggaaac uggugcgcac uauugagaga     1020

aaguauuaca aggaggaacu caaacagauc cuuaagaaac aaguggaguu ccacccugaa     1080

cugagggaua aggacuuguu ccuagacugc cugaaugagc ucuauccaaa caacgaugcg     1140

caccggaaug caugugccga ccgagauuuu aucuaccugu ucauggacga uauuuuguuu     1200

uaucagcgcc cccugaaauc uaagaagucc uugaucgcug acugcccuua cgaaaaacau     1260

auguacagag auaaggagac cggagagcuc aaauauucaa gcgucaagug uauugccaaa     1320

agucacccac uguuccagga auuuagguua uggcauuuca uaucuaacau ccggauuuac     1380

cagaaggagc gcguuaucga cguggaugca ugccaaccgg accuguccag cgcuagaucg     1440

gcccuaaaau cuaagcucga agcggaugua gacgugacaa augaguuucu gacuugugag     1500

gccgauuaug ucucccuuuu cgaaugguug aacaauaaga aaucagugag ucagaaggac     1560

uuucugaaau accccgcauu cggcaucaag aaaagcggcg acaacuauag guggaauuac     1620

guugaggaua aguccuaucc uugcaacgaa acccgugcuc ucauucuguc ucaccuugag     1680

aaggccaaua uacgagggga caucuuuaca cgggaacagg aggaggcucu guggcacauu     1740

uuguacagcg uggaagauag auacgagauc gucaaagccc uccagucauu cgcagccaag     1800

uauagguuua acgacgauuu cgugaccgua uucaguaaau uuccacccuu caagaaagaa     1860

uacggauccu auagcgcuaa ggcgauuaag aaacuguuac cucugaugcg cauggguaag     1920

uacuggucug agucagcaau cgacauaaaa acgcgggaaa gaaucgagaa gauuaucacu     1980

ggcgagugug augacaguau uagggaacgc guucgacuaa aagccauccg gcuccaugau     2040

auagagcacu uucgugcucu gccagugugg cuugccugcu augucgugua cgacagacau     2100

agcgaauccg gggauauuau gaaguggaag acaccccaag agauggacuc uuauauccag     2160

aauuucaaac agcacucguu gaggaacccg auugucgaac aggugaucac cgagacucug     2220

cgcguugugc gggauauaug gaagcaagua ggaagaaucg acgagauuca ugucgaacuc     2280

ggcagggaga ugaaaaaucc ugcagacaag cgaaaagaaa ugacaaagcg caucauugag     2340

aacgagaaua ccaaccugcg gaucaaagcc auguuaacgg aauuucugaa ccccgaguuc     2400

gaaauagaga augugagacc auacagcgcu ucccagcagg auauucuuag gaucuacgaa     2460

aacgguguuc uuaauucagu gagugagauc ccugaggaaa uuaccgggau ccucaagaag     2520

uuuaacgagc gugaacagcg caaacggccc ucuacaagcg agguccugag auauaagcua     2580

uggcuggaac aaaaauacug cuccccauau acuggcaaga ugauuccccu cggaaaacug     2640

uucaccccug ccuacgagau agagcacgug aucccacagu caagguauuu ugacgauagc     2700

uucaauaaca agguaauuug ugaagcagag gugaauaaac uuaaaggcaa cuugcugggg     2760

uacgaauuua ucaagaaaca ccauggacag cgaguugacu uaggucucgg cgagauuguc     2820

gauaucuucu cuguggacga auaugagaca uuugugaagg auuacuacuc cgggaaucaa     2880

cagaaaauga agaagcugcu uauggacgag auaccggaac aguucaucga gcggcagcug     2940

aacgauaguc gcuauauuag caaagucguu aagucuuugc ucuccaauau cgugagagaa     3000

caagacgagc aggagucaau uaguaaaaac gugaucguau gcacuggagg cgucaccgau     3060

aggcugaaga aagacugggg uauaaaugac guguggaaca agcuaauucu gccccgguuc     3120

cagcgacuca auaacaucac agaaaccacu gaguuuacgg cgaagaacau ggaagggcac     3180

gagaucccug uugugccauu cgaauaccag agaggcuuua auaaaaagcg cauugaucau     3240

aggcaccacg cuauggacgc cauagucauc gcaugugcca gccguaacau ugugaauuau     3300

cugaacaaug agucggcucg gaaaaaggcc agaaucucca gguacgaucu ucaaaaauug     3360

cugugcgaca agguucgcac agaugacaac ggaaauuaua aguggauuuu aaagaagccc     3420

uggggcaccc ucacucagga uguguacgca gcucuggaca acguaaucgu cucuuucaaa     3480

cagaaucuuc gagugauaaa caagaccaaa aauuauuacg ugcauuacga ggccgggaag     3540

aaaacaaucg cgaagcagga aaagggaagc ucaugggcca uuagaaaacc uaugcacaag     3600

gauaccgucu uuggugaggu uaaccugcgg aaagugaaga cuguaagucu aaaugaagcu     3660

aucaaaaacc cacaauguau uguggagaag gacuucaaga aaaagcuccu guccuugcug     3720

aaacagaauu uugaugcaaa gaaagucaag gccuauuucg aggaacauaa ggacgugugg     3780

ucugauauca acuuaagcaa aauagagguu uacuauuuua caaaggacac gaaagaaagg     3840

uucuuugcua cccgcaagcc ccucgacucc ucauucgaua aaaaggagau ugaaagcgug     3900

acugacacag gcauccagaa gauccugcuu cgucaccugg agcagaaaaa cggggauccg     3960

gacuuggccu ucaguccuga uggaauugaa gagaugaauc auaacaucgu ccaacucaau     4020

ggcgguagaa agcaccagcc aauagugaaa guucgggugu acgagaaggc agacaaauuu     4080

gcgauugggc agcaaggcaa caagucuucc aaauaugucg aagccgcuaa gggaaccaau     4140

cuguucuuug ccguauacga ggaagaggug agggauaaga aaacaaacgc aguuaugcua     4200

aagcgccacu augcuagcau cccccugaau guggccauug accgacagaa aaagggccuu     4260

ucgacugcac cugaggauga aaacgggaau guccccaucu ucgugcucuc accaaacgac     4320

cugguauacu ugccuaccgc cgaggauaua gcuaaugcgu cuauuaguuu ucccaucaac     4380

aaaaauagaa ucuacaagau gguguccugc acgggaaacc gguguuucuu uauuaagcau     4440

cacgucgcca auguuaucgu ggacaaaagc gaguucucug ugcugaacaa gauugaaaaa     4500

gcagaaaccg augagaugau aaaggagauc ugcgucccaa uuaaaaucga cagguuaggc     4560

aauaucguga agauuggugu uuauaaguga                                      4590


<210>  3447
<211>  4608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3447
augaacacca uccugggccu cgaccuuggg acaaauagca uuggaugggc ccugauaaag       60

guguugaacg auaaaacuag auuaccccug gagaucauug accuaggugu cguugugauc      120

ccuacggauc agaggacccu cgacaaguuc uccaaaggcg aaucuauuuc aaagacagcu      180

gagcggacua aacaaaaggg gguacgcaag cuguacgaac gaaagaaacu ucguagagag      240

aggcugcacc ggguguugaa uauccucgga uuucugccag aacauuauag uaaaaagaua      300

gauuucaaaa agcaccuggg ccaguuuuuc gguaacagcc agccgaagau cuccuacguc      360

gagaaugacc augggaaaca cgaguuuauu uucauggauu cuuaucgcga aaugcucaag      420

gaguuuucga aacauaacau caagaaugug cccuacgacu ggaccauuua uuacuuaaga      480

aagaaggcac ugucacagaa aaucagcaag gaggaacuug ccuggguuau auugaacuuc      540

aauaagaaaa ggggauauua ccaauuucgg gaugacaacg cggaugagaa uaaggaaaac      600

aaaauugaga aguacuaugc ucugaaagug acaaauguca agaaggaaga cgcccagaac      660

uacaccauca cucuagagaa uggcuggauu uauaaaaaca agaguaaaau cucccuccug      720

gauugggugg gcucuacaaa ggaguucauc agcaccgaaa aauuugacaa caaggggaau      780

auuauaaaga aaaagaacgg agaggacgau cgcacgcuua aacugguaga cuccgaaaag      840

gauuggaucg caauuaaaaa gucaacugag gaccgaaucg aaaauuacgg uaagaccguu      900

ggcacauauu uguacgagca ccuccugaaa gauccuaacc agaaguuaaa uggggaacug      960

auaaacauua ucgacagaaa auucuauaag aaugagcuca aacagauccu ugagaagcaa     1020

aaagaauuuc acccagagcu guugaacgaa gaucuauucc agaagaguau uaucgagcug     1080

uaccccaaga auagcacuag gcagaaagaa cucaagaaca aagaccugug cuaccuucug     1140

auugaugacg ugauauauua ccagcgggau uugaagaaaa agaacucuac caucaauaag     1200

uguuccuucg agucacguga guauuaccuc aauggagaaa aacaaauuca gaagaucaac     1260

ugcaucgcca aaagcaagcc ucuguaucag gaguuucgca cauuacaauu cauuaaaaau     1320

cugaaggucu acgaaauaau cgacggccua gagaaggaca uuaccaguaa acuccugcca     1380

gauuuugaga cgaaggaaca uuucuuugag uggcuuaacg acaaagaaaa gaucgaucag     1440

uugcagcugc ucucuuauaa auguuucgac cugcacgaua acgugaagaa ugaccuuauu     1500

gcuaagggua ucaaaagacc cacugagaag gcaauaagga cagaacuguu ggagaaauac     1560

cgguggaacu acuccaagga uaaugaccga aacuuucucc ugaaugagac ccgcuucaaa     1620

aucuuaaagu aucugaaaaa gguggauaag aaacuaacuc ucacaaacga acagauugag     1680

agccuguggc uuuugcugua cucggccaag gacgaaagag agcucaaaaa ugggcugcaa     1740

aaguuuucug aaaagaacaa guuaccuaag aaauucuccg auaucuuuau uucauuccca     1800

ccguuuaaga aagaguaugg agcuuacagu gagaaggccc ugaaaaaguu gcuuagccuc     1860

gucagguucg gcaaguauug guccuucaau aaaaucgaca acaauauaca gaacaagauu     1920

augaauaucc ugaacggcga aguugaugac ucuauuagcg accgggugcg cgagaaaacc     1980

auccuacugc aggauaagaa ugacuuucag gaucuccccc uguggcuugc gucauacgua     2040

gcauauaaca gauucaguga aguguccgag auaaaauacu gggacagccc ugauaagauc     2100

gaauuuuuga agcaacauuc uauugggaac ccaaucgucg agaaaauuau caaugagacg     2160

cugaaguuaa uaaaagacau uugggugauc cacggaaagu caugcaaaaa cuucuuuagu     2220

gaaauccaca uugagcucag cagggaaauc aaguccucuu cgaagaaacg ugcccagauu     2280

accaauaaca auacaaagaa ccagaaacug aauaaccuua uaaagagccu guugaucgag     2340

auggcuuccg auacuaaaua cgagaauguu acccccuauu caccuaguca gcaagaaauu     2400

cucaagaucu acgacgaggg uauuuauucu aaagaagaga acagcgaaaa gaucaaggag     2460

auaaauaaaa uccggaacaa uaacaagauu uccaaaucag auaucacaaa guacaaacug     2520

uggcuagagc agggcuauau uagcccauac acugggaaga ucauaccccu gaccaaacuc     2580

uucacagcca aauaucagaa ggaacauauu aucccgcaga ccaaauuuuu cgacgauucu     2640

uuuaauaaca aggugaucug ugaggcagaa gucaacaaac ugaaggacaa uucccuugcu     2700

augaacuuca uuaaggccaa aggaggcaag gcggugaaau ugagugaugg ugagacugua     2760

acgauacuga aucaagaaga guacaagcag uuaguucgaa ccagcuuuaa aaagaacucu     2820

aucaagcuca gaaaucugcu ucuggaggaa auuccugaca cauucacuaa aaggcagcua     2880

aacgacacca aguacaucuc caaacucgug cugucauugc ugaguaauau uguccgcgag     2940

aagggggaau uagagagcac agccaaacgg aucgugucga ccaacggcca aauaacuucc     3000

auccucagaa augauugggg acugaacaau auuuggaaca aacuuaucac acccagguuu     3060

gaacgccuga auaacauuaa uaaaaacaag aucuauggca auugggagaa caagaaaggg     3120

aagaacgucu uccagauaaa uuacuugaac gacaaucucu cuacgcugaa caccaaacga     3180

auugaucacc ggcaucacgc acuagacgcu cugaucgugg ccugcacuac acguaaucau     3240

aucaacuucc uuaauaacca guaugcuaag agcagaaauc agaggcacga ucuccgcgcc     3300

aaacugaagc ggagagagac cauuacaacu gaaggaucaa agaaacaagu uuuuaaggac     3360

uucaucaaac caugggagag uuuuacccag gaugcauuga agaaacugga aacgaccaua     3420

gugaacuuca agguaaauaa gaggauuauc aacaaaacaa uuaauuggua caagaaaugg     3480

gagaagcaga aagacgguac uuauaagaag auccagaaaa agcaaaccaa aggcgaugcc     3540

ugggcuauac gaaagccuuu acacauugac acaaucuacg ggaucgucug uaaaaagcuc     3600

ccccugaaau ccgcgcuuga gaacuauaag aauauuaucg augaaaaccu gaagcauaaa     3660

auuaaggagu ugaaagaagu gaacaaguuu gacaauaacc agauaaucaa uuucuuuaaa     3720

gagugcaacu ucauuuuuga agacaauaac auccagaagg uugaggugua cgauuucaag     3780

gacaaucgcg auaaaacuau ccucucuagc ucccggcagc ugauugacga gucauuuacc     3840

ccaaagaaaa uaggaaaggu ccuagauccc acgaucagaa acauucugcu uaaacaccuc     3900

aauagcacaa aguacaacaa uaaguucgac agugauggca acccuaucaa accagaaaag     3960

cuggcauucu cuccggaggg uuugaaagac uuaaauaaga acauuaaaca ucugaauaac     4020

aacaaggaac accaacccau caagagggug aaacucuuug agacccuggg gaauaagaua     4080

aaaguaggcu auacucagca gaagaacaaa aaguucgccg aaacagcuac cggaacuaau     4140

cuuuuucugg gcaucuacuc cgauaacaau aagcgugacu ucaaaaccau uccuuugauc     4200

cuagccauuc aacgcgagaa ggaggggcug aacccauguc ccgauacaga cgaaaaagga     4260

aaucagcucc uguuuagccu uucgccuaac gaguuagugu ucguuccaaa uaacgaggaa     4320

cggaagaauc ccaaccuggu cgauuuugaa aaucucucaa aagaccagau cgacagaaua     4380

uauaaguucg ugucuugcac ggguagugag gcauacuuug ugccguccag gaccgccaaa     4440

ccuauuaucg agaacgaaaa uggcacuaac aacaagagcc agcgaauuau cgaguucaau     4500

gaaaagacau cuaucaaaaa cgagaagaau gagccagucu ugauaaaaac cauuuguugg     4560

aagaucgaag uuaaccggcu ggggaaaauu aucaagguga uaaaguga                  4608


<210>  3448
<211>  4608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3448
augaccaaga uccugggccu cgaccuuggg acaaacagca uuggaugggc cauaagagau       60

acugagaaug aagguaucaa ccagauucug gacaaaggcg cuaggaucuu cuccgagggg      120

gugaagucug aaaauggaaa agagauuuca cgggcagccg aacgcacggc guaccgaagu      180

gcucguaaga ucaaauauag aaggaaguug cggaaguacg agaccuuaaa aguccugagc      240

auaaacggca ugugcccccu auccguugag gaaguggagc aauggaagaa aucugguuuu      300

aaggaauauc cucucaaucc agaguuccug gauuggcuuc gcacaaacga ggacaaaaau      360

aucaacccgu accuguuuag agauaaggcc ucgaagcaga aaguaacuuu guucgaacuc      420

gggagggcac uguaucacau ugcccagcgg cgcggauuuc ugucaaaucg acucgaccag      480

agcgcugagg gcguguucga ggaacauaac ccccaaaucc agaauuuaau ugaagaucug      540

gacaguucca acaccauccu uaaugaguug aaggaguacu auauaaaccu gggcauuauc      600

gaugaaacag agaaaucugg guuuaagaaa gaccuagaug aaggagagaa gaaacucaag      660

agccuguaca auucccuugu cgcaauuacc aagaaaaacg ccaacgacau cgaaacuugu      720

aagcaggagc ugaucgcuag auugaauaaa aaggaggauc ucgguaaagu gaagggcaag      780

auuaaagaca uaucacaggc caugcuggac aggaacuuca agacauuagg gcaauacuuu      840

uucagucugu auaauaaaga acggauccgu aaccaguaca ccagccgcga ggaacacuau      900

cucgaggaau uuauuaucau augccagacg cagggaauug agggcaucaa uacuaacgag      960

aaacuuccug aaaagaaguu caccggucug gcgaaggauu uguacagagc aaucuuuuuc     1020

caaaggccac uaaaaucuca gaaggggcug auuggaaaau guuccuucga gaagaauaaa     1080

ucacggugcg ccguuagcca ucccgacuau gaagaguuuc gaauguggag uuaccucaac     1140

acaaucaaga uuggcacuca gucugagaag acccugcgcu uccuuacacu ggaagagaaa     1200

uugaagcucg ugccuaaauu uuauagaaag uccgauuuca auuuugaagu ccuggcuaaa     1260

gaguuagugg aaaagggcgc cagcuucggg uacuacaaau cgucuaagaa gaacgaguuu     1320

uucuauuggu uuaauuacaa accaaccgac uccguaucag cauguguugu gagugcuagc     1380

cuggagaacg ccauaggaaa ggauuggaaa aucaagacgu ucgaauauca aacuaggaau     1440

acagagaaaa acgaagucac caaguccgug gacuacaagg aucuauggca ccuccugucu     1500

guggcgacua gcgacacaua ucuuuacgau uuugccauug agaaauugaa gcuggaaccc     1560

aaaaaugcaa aggcuuucuc aaaaaccaag cucaagaaag acuuugccag ucugucccuu     1620

gcugccaucg caaagauccu gccuuauuug aaacaggguc uccuguacag ccaugccguc     1680

uucauggcua acauugagaa cauaguugau gcggacaucu ggaaggacga ggaacagcag     1740

aaauucauuc aaucuaagau cguggaguua guagauaauu acauugugga aaagucaaaa     1800

cuggagcuaa ucaacggccu ccugaagaua uauaauacgg aagacaaaga ggggcggaag     1860

gucuacuaua guaaagaggc agaaagccuu uuugaggccg auuugcgcaa gaaacuggug     1920

ccauucuaca aggcuaacau cauuaucgag gaacacgagc aggaaauuau cuuucaggac     1980

cucuucccga uacugaugga ucaguuaaag aaacaagagu uuauuaagau cggaagacug     2040

gacaaacaga uugaagccuu ccuugagggc gaaaaugagg agggucagau cuuuugcaac     2100

cacaccgaua aguugaaaaa gcucuaucau cccuccgaca uagaaguuuu caagaaaaag     2160

acaaucaaag augagugggg gaaugaaaag gugguccugg gcucuccucu aacuaccucg     2220

auuaaaaacc caauggcaau gagggcccug caccagcucc guaaggugcu gaauacacuu     2280

aucgcuaacg accaaauuga ugaggacacu cggauccaua uagaaauggc ccgagaguug     2340

aaugaugcaa acaagagaaa aggaauacag gacuuucaga augagaacaa gaaauucagg     2400

gaagaggcga ucaaggaaau caaaaagcug uacuuagagg aaugucacaa ggacguaaau     2460

cccaccgagg augacauucu ccgcuaucag cuguggcuug agcaaggcaa augcgaaauc     2520

uacgaggaag ggaacaacau uagcauaugu gauaucauug gauccaaucc uucauacgac     2580

aucgagcaca caauuccacg gaguaucucu caggauaaca gccagaugaa uaagacccug     2640

ugcuccuuga aauuuaacag aucaauaaag aaacaaaaga ugcccguuga gcucagcaau     2700

uauaacgaca uccugccgag gauugcucau uggaagaaag aagccgagga acuaacucgc     2760

cagaucgaga cgauuucucg auccaucaag agugcagcca caaaaguggc uaaggauaaa     2820

aauauacgga agcgucacua ccugacccuc aaaagagacu auauucuggg uaaguacgaa     2880

agguucacuu gggaggagcc uaaggucggc uuuaaaaaca gccagauccc cgauaccggg     2940

aucauuacaa aguaugccca ggcauaccuu aaaucuuauu ucaagcgcgu ggaauccguc     3000

aaaggaggcg cuguggccga guuccggaag uuguggggua uacaagaauc auuuaucgac     3060

gagaauuggu ggaagcauua caaagauaag gacagagaua aacacaccca ucacacuauu     3120

gacgcgauca caauugccug uaugccaaag gacaaauacg aucuguuagc ucacgcaugg     3180

aggcucgaag acgagcagga uaagaaggcc gcuaaaguuc ugaucgagca ggccaagccu     3240

uggaaaacgu ucaaggaaga cauagagaaa aucgaaaccg agauucuugu gagucauuuu     3300

acucccgaua acguaaagaa gcagagcaaa ucgaucauua agaaucgagg gaaaaagguc     3360

uaugugcuga aaaacgaacu accaguuaau uucaagaaca aaaucgaggg caaggacuac     3420

uuuaagcuca aauucgauuc caagauacug uauaaaauuc ccaagaaaaa ggagaagcaa     3480

acagacaccu uuuacgaaga guugccuaaa aauuaucuga acggagugga aggcaaggau     3540

uacuucaaaa ucaacacaac ugggaagacc uuuuauaaaa ucccaauuuu caaucaggga     3600

gacacgaucc gcgguucuuu acaccaggag accacauacg gcgcaauaaa gcucccggau     3660

auugacaucg agacuaagaa accccugcau accgacaaag ggggauuuau ucuuaaaaag     3720

gauaucaaag gcaacgaaau agucuucuuc gugguacgga aggagauuag caagaucuca     3780

gaaaaugacg ugcagaacau cguugauaau gucgugagaa agaagauuga gaacgcgauc     3840

gccaauaguc ugauuacauu uaaaauagug aagaaaaaga aagucgcugu uaucaaggaa     3900

ggugugacua uuuggaugag ggagccuaac aucgagaagg ggaucgaagg cauuccaauu     3960

aaaaaggugc guaucauuac caauuccguc aaaaacccca ucgagauuaa gguacacucu     4020

ccuuugagca aaucccgcca caagcauaag caaaaagugu acggacagaa ugacgaaaac     4080

uaugccaugg cacucuacga gcuggauggc aagcgggagu ucgaacuaau caauaacuuu     4140

aaucuggcua aacuucucaa gcagucacaa agcuauuacc cacugcacaa agagaaggaa     4200

auaaagggga aaaagaucuu gguucccauu gagaaaagaa acaauaagga cgugauccug     4260

aaaaggggac agcaggucgu guucuaugau aagacgguug aaaacccgaa ggacauuagu     4320

gagaucauag auuuucgaga acggauuuac aucauugagg gcuuaacaau ccagcgccaa     4380

aaagacaaga aaaccucuaa ggugaacgag uaugguauaa uccagcucag acauuucaaa     4440

gaagccagga aguccgagga aauuagcaag gauaauuuua aaccugacgg ggaguucaag     4500

aucaacgaga auaaaccaac ucggaagaug aaccacaauc aguuuacagc auucguagaa     4560

ggaauugauu uuaaaguccu gcccucgggc aaguuccaga aaaucuga                  4608


<210>  3449
<211>  4614
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3449
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugacaacu       60

aaugauuucg acacgaagaa agguaccaua gagggcuugg gguccagaau cauucccaug      120

ucucaggaua ucuuaggaaa guuugacuca ggcgugaguc ugagccaaac agcucagagg      180

acugaauacc gggguaaacg ccgucuaguc cagcgauucc uccugagaag ggagcggcuu      240

caccgcguuc ugcauauuuu ggaacuccug ccuaagcagu auuccgaggc aaucgauuuu      300

gacaagaaac uggggcaauu caagccagaa agagagcaga aaauaucuuu uuacaagaac      360

gagucgggca aaaaucaguu caucuuuacc aagucacacc aggaaaugcu cacagaguuc      420

caaaccuauu acccgacuau uaagaacauc cccuaugauu ggacaauuua cuauuuaagg      480

aaaaaggccc ugaccgagaa aaucagcaag gaggagcuug cguggauauu gcugaauuuu      540

aaccagaaac ggggauacua ccagcuacgc ggcgaagaga aggaagagaa uaaaaacaag      600

aaggaagagu uccauacgcu ccuggugacu gacauuaucg agcgagauaa agggaagagu      660

ggaaccuggu auaauguaau ucuugaaaac gguuggaucu acaaaagaga guccaagauc      720

ucucuggcug acuggauugg caaaacaaag agcuuuauag ugacuaccga auauaaugau      780

gacggguccg ucaaguugac aaaagauaac gaggaaaaga ggucauuccg gaguccuaaa      840

gaggacgacu ggggacucau caagauucgu accgagaaaa cgaucaauca gagcgauaag      900

ucugugggcg ccuacauaua ugacgcacug uuaaagaacc caaaucagaa aauuaagggu      960

gaacugguuc gcacuaucga gagaaaauac uauaaggaug aacucaggga gauccuuaaa     1020

aagcaacugg aauuucacuc cgacguguug aacgauaaga uucuauacaa aaagugcauc     1080

gacgagcugu acaaaaacaa ugauucacau aagaacagca uuaaagaccg gggguucgau     1140

uaucucuucc uggacgauau aaucuuuuac cagcgacccc uuaagaguaa aacaucucug     1200

auuuccgacu guagcuugga guuccgcccu aucaagaaua agaacggagu ccucgugaaa     1260

gacaccgaug gcaagcaaau cgccaaagcu cugaagugca uugcaagauc gaauccaaua     1320

uuucaggagu ucagguuacu gcaguuuguu aaaaaccuaa agaucuauaa gcggggcgua     1380

guggacgaug ucgaggugac uaaugacuac cucaaaucug aaaacgaugu ggagaagcug     1440

uucaauuggc uuaacgacaa agccgagauu aaucagaagc aauuuuuggu caacuucaaa     1500

cugaaggaag auaagcaccg cuggaauuuu guugaggaca aaggguaucc cuguaacgaa     1560

acaagaaauc aguuccucaa cgcuaucgcc aagauugaug uggacaaauc cuuuuucaac     1620

accaagaaua cgcaggaucu guggcacauc cuuuacucag uaaccgacaa aauagagauc     1680

acaaaggcga uuacuaccuu ugcaaagagg cauaaccugc cuaaugaguu caucauuaac     1740

uucaguaaaa ucaagccaua uaaaaaggaa uacgguagcu uuuccgagaa agccauaaag     1800

aaauugcucc cgcugaugcg uuucggaucu uacugggaug cuaagaauau ugacaucaag     1860

acauuagacc ggauugauaa aauccagacu ggcgaauuug acgagaagau acgaaacaga     1920

gugagggaaa aagccaucga ucugcacaag auugacgauu ucaaagaccu accccucugg     1980

cuggcaagcu auaucgucua cgauaagcau ucagaggcua gugacguguc caaguggaaa     2040

accccugccg auauugaaaa guuucuuaaa cacgaguuca agcaacacag cuugcgcaau     2100

ccaaucguug aacaggugau aacagaaacc cugcgggucg ugaaagacau uuggcuccag     2160

uauggggagg gaaaggaaaa cuuuuucaau gagauccaua ucgaacuggg cagagagaug     2220

aagaacgaua aagcgacuag ggagaagauu ucuaaaaagg uaucagaaaa ugagaacacg     2280

aauuuacgca ucaaagccau ucugacagaa cuuaagaacg acgguguuag ugauauacga     2340

cccuacagcc cuucccagca agagaucuug aagauuuaug aggaaggggu guacaauaac     2400

gagucucgga aagaaaagcu cgaggaaguc gacaaaaucc guaagaacgc aaaaccaacc     2460

acuucggaga uuaucaagua uaagcugugg cuagagcagg gcuacgcuag ccccuacacc     2520

ggaaaacuga uaccgauguc cgaacucuuu acaaccaagu aucagaucga gcacauuauc     2580

ccucagucaa gauucuuuga cgauagucug ucuaauaaag ugauuugcga aagcgagguc     2640

aacgaacuua aguccaauau gacugccuug gaguucaucc ugacaaacga gaauaggaua     2700

gugacguuaa ccggcgggga aacuguuaaa auuuuuacaa aggaggcuua caaccaacau     2760

aucaucucau auuacagcaa aaauaagaac aagcagaaac gccuccugac cgaagagauu     2820

cccgaacagu ucauaucucg gcaacuuaau gacacaaagu auaucuccaa aauugugaag     2880

aaccuguuga guaaaaucgu aagagaggag gaugaacagg aggucacuag caagcacgug     2940

guuucucuca auggauccau uaccucaagg augaagcagg acuggggucu gaacgaugug     3000

uggaauacga ucgucagucc acgauucgaa cgccuaaaca cccugacaaa uagcaacgac     3060

uuuggccagu ucgagcucaa aaaggaugcc uacgggaacg agggaaaacg gguguuucaa     3120

acuaccguac cugacgcaau agccaagggc uucacuaaaa agagaaucga ucauaggcac     3180

cacgcucugg acgcgcuugu gauugcaugu guuaaucgua cacauaucaa cuauuugaau     3240

aaccugaaug ccaaggauac cgacgacaaa gcugucaagc acgaauuacg caacaaacuc     3300

ugcuuuaaga cgaaacccga uucgaauggu aacuacaagu gggaguucca uaagccaugg     3360

gaaaaauuua caaaggaggc ccaggacaaa cugaauacca cuauuguguc cuucaagcag     3420

aacacacggg ugaucaauaa aaccgucaac aaguacguuu cuuauaaaga ugaaaauggg     3480

aaccuuaaug uggacgcaaa cggccugccc cagaagaagg ugauaacuca aaccaaagga     3540

gauaauuggg ccauuagaaa gccucuacac gcugagacag uaagcggcaa agucuuucuc     3600

aagaggauca aacagucaca caucacgauu gccaacgcaa ucgaacaggu ggaacugaua     3660

guugacaagg aggugaagaa acaguugaag gcgaaaauua agcuguaccc aaacaauauc     3720

gcugggcuca aaaagcauuu aaaggccuuc ccggucauga uuaacgauaa agcaaucgac     3780

aagauacaaa uuuaugaaac ccuggaggcc acugcuacac gaaaaauccu ugauaucacc     3840

uuugacgaaa agaaaauuca gaagaucaca gauacuggaa uacagaagau ucuguugaau     3900

caccucaaac aagagaagua ccagaacacc guggacgaga augguaaaaa gcuggcaccc     3960

caugaaaucg ccuucaguga gaacggccua gaagaucuga auaaaaacau uacguuccuu     4020

aaugacggga agaaccacca gccuaucaaa aagguucgga ucuuugagga aggaaauaag     4080

uucccacucg gcgagguggg uaacaaaauu aauaaguuug uagagacagc uaaagggacc     4140

aaccuguucu uugccgucua ugaaaaugac gauaagaaac gcaacuacaa gacuauaccc     4200

uucaacgagg ugaucgaaag acagaagcaa ggcuugaaug cggcccugga gauugacgag     4260

aacggaaaua gguuacucuu uacccugucc ccuaacgauc uuguguauau cccacuggaa     4320

gacgagauug gcuacuugaa uggggucuac uucaaaaacc ucaauaagga acagacaguu     4380

cggaucuaua aauuuguguc uuguacugga ggcgaggguc auuucguacc caacaauuac     4440

agcaaggaaa uaaucuccaa cgagaauggg ucaaacaaua aaaacgagcg aaugcugaau     4500

uuuaacaacu ucaauauuau cuuugaugga aaggaaaagc cggugaugau uaaaagcauc     4560

ugcuggaagc uagaggucga cagacugggc aaagugauaa agauuaucaa auga           4614


<210>  3450
<211>  4617
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3450
augaagcaca uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaaa       60

uccgagcagg auugcgacga ugucgaaaga auaaauggua ucgaggcugc aggcucuagg      120

auuaucccca uggacgccgc gguuuugggg gauuucgcua agggaaacac aaaaucacaa      180

acugccgacc ggacggcagc ccgcggcauu cgacguaugg gugaaagaca uaaguuaagg      240

cgggagcgcc ugaauagagu gcuagaugua augggguuuc ucccugaaca cuacagugcu      300

gcacugacca gguauggaaa auucaaggac ggcgaggagu guaagaucgc cuggcggaaa      360

gaugaaagcg gccaguggac auuuguguuc gaggacuccu uucgcgaaau gcuugcugau      420

uucgucgccc aucagccaga guggcuggca gggggacgaa aggugccgua cgacuggacu      480

auauauuacu ugaggaaaaa ggcacucucu aaaccccuga ccaagcagga gcuggccugg      540

cucuuacuga acuuuaauca aaagagaggu uauuaccagc uucggggcga agaugagcac      600

acagacgaca aagggaaguu gguugaauau uacgcucuga aaguggucgg aguggaggau      660

accggcgaca agaaagguaa ggauacuugg uucaacauca ugcuagaaaa ugggauggua      720

uaccgucgcc cugccgcaga ggcuccagac uggauuggaa aaacaaagga guuuaucguu      780

accacggauc ucgaaaagga cggcacuccc aaaaaggaua aagagggcaa cauuaagaga      840

ucguucagga ugccuaaaga agacgauugg acccugauca agaagaaaac agagaaugac      900

auagaucggu cagggaagac ugugggagaa uauauuuacg acgcccuucu ggcggaccca      960

acccagaaaa uccgagguaa guugguccgc acaguggaga gaaaauauua caaggaggaa     1020

cucaagaaaa uuaucaacag ccagaagagg uuuauccccg agcugaauga uaacgaauua     1080

uauggccggu gcauuaguga gcuguaccaa uccaaugacg ccuaucgcca gucuauaagc     1140

uccugugauu ucaccuaccu cuuuguggac aacaucauuu ucuaccagag accucuuaaa     1200

ucaaagaaaa gucugaucga uaauugccca uaugaggaac auagcuacac ggacucuaag     1260

acuggggagg ucaagacagc accgguuaaa uguguggcua aguccaaccc cuuguuccag     1320

gaauuuaggc uauggcaauu cguacagaac auacguauuu uugagcggca gaaaaagauc     1380

ggagauucag ugaaaaccga cgucgaugug acuggcgaau uuaucaagag cgccgacgau     1440

guugugaguc uguuugacuu ccucaaugau cgaaaagaca uuacacaauc uguccugcuu     1500

gguaccuauu uuaagaucaa gaaaccuaag gggaaagacu ccgauuaccc auauagaugg     1560

aacuacgugg aggacaagac guaucccugc aaugagacca gggcucugau uuugaaaggc     1620

cucaaggaug ccggaauaga cgaaggcuuc cuggauaagg acaaagagua cagcuuaugg     1680

cacauccugu acucgguaga agauaagcag gagauuacaa aagcaaugga aaaguuugcc     1740

gcuaaaaacu cucuaggggg aaaguucgcg gagguuuucu ccucacuccc uccauucaau     1800

aagaacuaug guaguuacag cgagaaagca aucaagaaac ugcuugccuu gaugcgcacu     1860

ggcaaguauu gguccgcuga cgccaucgau aaacacaccc uggaacggau ugacaagaua     1920

aucaaugggg aguacgauga aucuauuaga acaagggugc gcgagaaggc aaucaaccuc     1980

acugacguca gccauuuucg aggacugccc cuuuggcugg ccuguuauau uguguacgau     2040

cggcacucag aaaguaaaga cauaaccugc uggaauuguc cggcugacau cgaucaguau     2100

uuggaccauu ucaagcagca cucccuccgu aacccuaucg ucgagcaagu gguuacagag     2160

acccugagaa cugugaggga uauuuggcgc caguacggca aaaucgacga aauucacauc     2220

gagauggguc gggauaugaa gaaucccgcc gacguaagag caaaaaugag caagaggaug     2280

auagauaacg aaaaugcgaa cuuacgaauu aaagcuaugc uggccgaguu cgcagaccca     2340

gauuuuggga ucgccggcgu ccgcccuuac ucucccucac agcaggaaau gcuacggauu     2400

uaugaggagg gagcucucga aaguguggac gagguuccag augaaaucaa ugagauaauc     2460

agcaaguuca accaaacgga cauuaagaaa agacccacac auggcgagau cauuaaguac     2520

aaacuguggc uugaacagaa guaucagucc ccuuacaccg ggaggacuau cucuuugucg     2580

cgucuguuua ccgacgauua ucagauagag cacgugauuc cacaaagccg cuacuucgac     2640

gauuccaugu caaacaaagu caucugcgaa gccgagguga aucagcucaa ggaccggaug     2700

cugggauaug aauuuaucaa ggcacaucac ggugagaaag uauuacugag uucuggcgag     2760

acagugacca uucuuacugc ugaagaguac gaaagauugg uucagcucaa cuucaggaag     2820

aauccgguca aaaugaagaa acugcuaaug gaugacaucc ccgauaaguu uauugagcga     2880

cagcugaacg acagccggua cauauccaag aaaaucaagu cacuccugag caauauggug     2940

cgcgaugaag gggagcaaga ggacauuucu aaaaacguga ucgccuguaa uggagcgauu     3000

acagauagac uuaagaggga cuggggcguc aacgauguuu ggaaucauau cauauugccu     3060

cgguucgaac gacugaacgc cgugacgggu uccgacagau uuaccacuac aaguaccaau     3120

gggcacacaa ucccagacau gcccuuaaac cuccagaaag gcuucaauaa gaagcgcauu     3180

gaucacaggc aucacgcuau ggacgcaauc gugauugccu gcacuacccg uagccaugua     3240

aaccugcuua auaacgaggc ugccaugucu aaaaacaaug caguccggua ucagcugucc     3300

agaaaguuga ggcgcuacga agauacgaug gugacccgaa acggagagac aagaacuauc     3360

ucaguugcgc gggaauuucu caaaccuugg ccaaccuuca augccgacgu ggagaaggcu     3420

cugaggggca uagucgugag uuuuaaacaa aaccuacgcg uuauuaauaa gacagugaac     3480

aaguaucaga aauucguaua caaugaggaa aagcacauca aagagaagaa agccguccgu     3540

caggaagaga gaaacgggcg gcaucugcuc gcaaucagga agcccaugca caaagauacu     3600

guguauggag aggugaaucu gcgccgaauu aaggaaguca gccuuaagga cgcuguugcc     3660

aacccgaaag cagugguaga uaaggacuug aaaaagaaac ugauggccau gauagaguua     3720

ggcuacgauc ucaagcagau caccaaguau uucgaagaca auaaagaugc uuggcaagac     3780

gugaaccuga agaaaauuaa ggucuacuac uuuacgaaag auacagguga cagauauuuc     3840

gcgacccggu uugggaauga ccuugugacu cuguucucga agaucacaaa ggccgaugac     3900

gcgaaaaagc agauugagag gaucaccgau acuggaauac agaaaauccu aaagcgccac     3960

cucgaagcua acggcgguga cugccagaaa gccuuuuccg ccgauggcau ugacgagaug     4020

aauagaaaca ucacccaacu gaacggggga aggccucaua agccaguuua caaggugcgg     4080

auuuaugaga aagcugauaa guucgcaauc ggcacaaaag ggaauaaggc cgcgaaauuu     4140

gucgaagcug ccaagggaac gaacuuguuc uuugcagugu accugaaaga guaugaagac     4200

ucuaccacug gcgagacaau aaagaagcgu guuuucgaua ccauucccuu aaaaacagug     4260

aucgaccgcc ucaagaaugg ucagacuccu gcccccgaag agaaagaugg ggcuaagcug     4320

cuuuuugucc ugagcccaaa cgacuuggua uaccucccua ccgaggacgu gguuaaaaau     4380

ggaacgauca accgaccccu ggauaagaga aggauuuaua agaugguguc auguacaggc     4440

accgaaugcu acuacauccc auucauaguc gcaaguccga uuguggacaa aguagaguuc     4500

uuuucccuaa auaagaucgg ucgggccauu acuggcgaaa ugaucaaaga gauauguauu     4560

ccuaucaagg uggaucgccu ggggaacauc accgaaguca auaacgagaa aauuuga        4617


<210>  3451
<211>  4623
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3451
augaacaaga uccugggccu cgaccuuggg accaauagca uuggaugggc ccuggugggu       60

acagaugagg aaggcaaccc cauaagaauc uacaaauccg ggucuaggau uaucccuaug      120

ucacaggacg ucuugggaaa uuucgaucgg ggcaacagua uuagccaaac ugcugagcgc      180

acguccuauc gagguguucg uagauuaagg gaacggcauc ugcuacgcag agagaggcuc      240

caccgggugc ugcaccuuau gggguuucug ccaucucauu acgaagaggc aaucggaugg      300

gacaagggcg auaauaaaac cuucgccaag uuuuauucgg acacagagcc gaaauugccc      360

uggcgcaaga acgcggaacg agagcacgag uuccucuuua auacuccuua caacgagaug      420

cugguagauu ucagaaagaa ucagccacag cuguuuaaaa uaaagaaaaa cggccaggag      480

ucacucaucc ccuaugacug gaccauuuac uauuuaagga agaaagcucu gagcaagccu      540

aucaguaaga aagaacuugc cuggauuauc uugaauuuca accaaaagcg gggguacuac      600

cagcugcgug gagaggaaga ggaagaaaau uccaacaaac uaauagagua ucaugcacuc      660

acagugguca aggugauugc cgaugaacca cagaaaggua agucugacau cugguacaau      720

gcugagcugg aaaacggcug gguuuaucgc agaagcucca aaacuccccu uuuugauugg      780

gagucaaagg ugaaggaguu cgucgugacc acagaccuga acgcagaugg gaguuugaaa      840

guagacaagg agggaggcgu uaaaaggagc uuucgggugc cuucugagga cgauuggaug      900

cucgucaaga aaaagaccga auccgacauu gagaagucag auaaaacggu ggguugcuac      960

aucuaugaca cuauccugca gaauccaagc caaaagauuc gagggaaauu agugcgcacc     1020

auagaaagaa aguucuacaa aaaggagcug acagaaaucc ucaacaagca gaaagaguuu     1080

cacagugagc uucagaauaa ggaacuguau caggagugua uuaaagaauu guacccgcac     1140

aacgaggccc auuacgauuc ucuacugguc aaggacuaua cugaucucuu cguuaaugac     1200

aucauauucu accaaaggcc ccugaaaucc aaaaagagcc uuauuucgaa cugcccuuau     1260

gaguaccggg uguaucgcaa ugauucugga aucguagaaa gagugccaau caaguguauu     1320

gcuaaguccc acccccugua ccaggaguuu agguuguggc aguucaucca uaaccuccgu     1380

auuuaugaac ggcaacgaga ggucaauggc aaaccugaaa ccgacgugga uauaacagcc     1440

gaguuuaucc caaccacgga ggacagaguu aagcuguucg acugguuaaa caauaaaaag     1500

gaaauuaaac aggauacucu gcuacaguca uacuuuaaga ucaagaaaaa gaaaggcgac     1560

gauaaguucc ccuacaggug gaacuaugug gaggacaaag aauacccgug caaugagaca     1620

cgcgcgcuca uccugagucg gcuuaccaag uugaaccuga gcgaagauuu ucucacuaag     1680

gagauggagc uggcacuuug gcacauucug uauuccgucg aagacaaaga ggaaauaaag     1740

aaagccuuca agucuuuugc ucagaaacac aauuuggaug acagcuucgu ggaucaauuu     1800

aucaaguucc cucccuuuaa gaaagacuac gggucauaua gugccaaggc aauuaaaaag     1860

cuccugccau uaaugagaau cggaaaauac ugggaugaga aggcuauuuc cgguaacaca     1920

aggagccgca uccugucuau aaacgaacga cuagaggaca ugcggucaag uggcaagaaa     1980

aucgaggaug uaagcgacga cgauauuccu aagcagguuc ucaaauccuu cgacaaggug     2040

aauggggucu auucuggacu gaacaccuac caggccuguu acgcggugua uggccgucau     2100

ucggaagcca gcgagaucac gaaauggaag acaccccagg auauugacga uuaccuuaau     2160

gaguucaaac aacacuccuu gagaaaccca aucgucgaac aggugauaac cgaaacucug     2220

aggguugugc gcgacauuug gaagaaguau ggugauuuuu cagaaaucca uauugagcuc     2280

gggcgggaaa ugaaaaaucc cgcagacaag agagcuaaaa ugaccacaca gaucagugag     2340

aacgaaaaua cuaaccugag gaugaagucu uuacuggccg agcuuaaaaa ucacaacgau     2400

guagagaaug uccgaccuua cagcccaucc cagcaagaaa uauugaagau cuaugaggaa     2460

ggcgugcuca acucaguuag cgagauuccg gaagagauca uuaagaucac caaaucuucc     2520

cagcccaguc agacagagcu ggugcgcuac aagcuauggc uggaacagaa auaucggagc     2580

ccuuacaccg gagagauaau uccacucggc aagcuguuca cuucugcuua cgaaaucgag     2640

caugucaucc cccaauccag auauuuugac gauucacuua guaauaaagu gauuugcgaa     2700

agcgagguaa acaaguugaa ggggaaucug uuaggauacg aguucgugaa aaaccaccac     2760

gguguuaagg ucgaacucaa cuuuaauaaa acgguggaga ucuucucgau uaacgaauau     2820

gaggaguuug ugaagggcaa uuacgggaaa aacaggggaa agaugaagaa acugcuucug     2880

gaagacauac cuaaguccuu caucgagcgu caguugaaug auacacgcua uauuucuaaa     2940

gucguuaagg gccuccugag caacaucgug cgggaagacg gugagcagga agccaccuca     3000

aaacauguga uuccauguac ugggggcgua accacaaucc uaaagcaaga cuggggacug     3060

aaugaugucu ggaacaagau agugaccccc agauuugaga ggaugaauga gcucacuaac     3120

acaagucugu ucggccagug ggaaaauaaa gaggggaagc gaaucuuucg gacggaaguu     3180

ccgcuugagu ugcagaaagg auucaacaag aaacgcauug accacagaca ucacgcaaug     3240

gaugccaucg ugauugcuug cgcgaccagg ucccacguca cuuaccugaa uaacgaaucu     3300

agcaaggcaa augccaaaga gauccgggac gguuuaaagc auaagcucug uuuuaaaucc     3360

aagacagaug agaccggcaa cuauaaaugg caguuccaca agccuuggga cacauucacu     3420

caagaugugc aggacacccu ggaaaacaua guuauuucau uuaaacagaa ucuucgagug     3480

aucaacaaga cgaccaauag auaccagaag uacgaggaag agaaaaagau ccugaaaagc     3540

caagaaaagg gggauaguug ggcuauucgc aaaccacuac auaaggacac aguauucgcc     3600

aaggucucuc uccagaaaau aaagugcgug aaacuggcaa uggccuugaa ggauuggaaa     3660

aacaucgugg acaagcagcu gaagaaagag auuaggcguu uaauccucuc cuauggaaag     3720

uuugacgcug agauucugaa uaaauacuuc aaggaucgga aauauaucuu uaaggacguc     3780

gauauaagca aaguugacgu guacuauuuc gaaacugagu acguagccac cagaaagaac     3840

cuugauacau cguuugacag gaagucaauc gaaucuauua cugauaccgg cauccagaaa     3900

auucuguuga aucaccucgg ugugaagaac aauaaccccg agcuggcauu cagugcggag     3960

gggaucgacg aaaugaauca acgcauaguc gagcuaaacg gcggaaaaca gcaucagccu     4020

auuuauaagg ugcgaaugua cgaaccccug ggcaauaaau uucaaguugg gagaaucgga     4080

aacaagaagg auaaauacgu ggaggcugcc ccaggcacga aucuuuucua ugcaaucuac     4140

caguccgaag acaagaaacg gauuuaugau acaaucccuc ucaacauaau uaucgagagg     4200

cagaagaaag agcuguuucc cgucccagaa aauaaggagg acaaggauaa cuuguacaaa     4260

cuguuauucu ggcucagccc gaacgacaug guguaucugc cuaccgaaaa ggagugggcc     4320

aaucccgacc uuuucaacuu uaauaacaug acuaaaaauc agcgcaagaa acuguacaag     4380

uucauugaug guucugggac aaccgcuaac uuuaucccaa auacuguugc caacauaauu     4440

uucagcaucc ccaagaaaga cuccggaaag uauucaaugg gcaaucguau cgugcaaaac     4500

gaauuuggua uuggcagucc ucagagcaaa aaucagaagg cauucaccgg ggagaugauc     4560

aaagagagau guuggaaguu gaaaauuaac cggcucggag auauaaucga augcauuaag     4620

uga                                                                   4623


<210>  3452
<211>  4629
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3452
auggagaaga gaauccuggg ccucgaccuu gggaccaaca gcauuggaug ggcccuggug       60

aaaggugaaa ggcacgagaa ugauaacgaa acaauauugu ccuacaucga ggcugcaggc      120

ucucggauuu uaccccugga cgccgcggcu cagggggauu ucgaaaaggg aaauccugcc      180

aaaacucaaa cgucagagcg cacccgaaug cguggcauga gacaucuacu cgagaggagu      240

cugcuucggc gcgaaagacu gcacaggguc uuggacauca uggauuuucu cccaccgcau      300

uauagccagu cccugacacg guacgguaag uucaaagcag acacugagug caagcuggcc      360

uggcgcaaga ccgaaacagg gcacuaugag uuucucuucc aggauucuuu ucaggagaug      420

uuacaagacu uccagaacca uggaaaacug gaagagaaua agaaaauucc cuacgauugg      480

accaucuauu accuucgaaa gaaagcucuu acucagccug uuucgaagca ggaacuggca      540

uggauacuac ucaacuuuaa ucaaaagaga ggcuauuacc agagggagga ccaggccgug      600

gaugacaacc agaaaaagcg gguagaauac caagcucuga aagugaucuc agucgaggau      660

acaggcgaga agaaagggaa gaauaccugg uaugacgugc gucuugaaaa cggacugguu      720

uaccacuaua gcgccccaau uaaacccgac uggaagggug agacgaagga guucaucgug      780

acuaccacaa uugaucagaa uggcaaaccu gaauuggaca aggaugggaa accaaaguac      840

aguaugcgcg cgcccaaaga agacgauuau aagcuccuga uggagaagac uaaaaaggac      900

aucaccgauu ccggaaaauc ugucggcgag uacauauaug acgcauuacu ggugaacccu      960

cagcaaaaga uuagaggugg gcucguaagg acaaucgaac gguacuuuua caaaaaggag     1020

cuugaaaaga uucuguugag ccaggagcga uucaucccag aacuaaccga ucgcgagcug     1080

uauagaaggu guguugagga acucuacccg uccaaugacg ccuaucggca ccugaucgag     1140

ucacgcgaua ugagauaccu ucugguggaa gacauuuugu uuuaucagag gccccucaaa     1200

aguaagaaaa gccugauaga cgauugcccu uacgagucuc guuccuaugu cgaccgggag     1260

acgggagaaa ucaagucaag cccagugaaa uguauugcuc agagucaucc cuuauuccaa     1320

gaguuucgac uguggcaguu cauccacaac cuaagaauau accagaauga aaggucugug     1380

gagggcaagc uccagcugga ugucgacguu acugaagagg ugcuuccuac accagagucc     1440

aaggccgcau uguuccgcuu ucugaacgau cgggaaaacg uaacccaaga gauucuccug     1500

aaagaagugu ucaagggcag aaaacuuagc gucaaggacu gccccuacag guggaauuau     1560

gcuggggagg guaaaaagua cccguguaac gaaacucgcc aucugauguu gaaacuccug     1620

aagaaggccg gaaucgauac agcguuuuua accccugaga aagagcaggc ccuguggcac     1680

aucauguauu cgguggaaga caaggaucag uacgagaaag cacuaucuac guucgcucga     1740

aagaaugacc uccccgaugg cuuuaaagaa caguucaaga aggccccaac cuuuaaaaag     1800

gacuaugggg cuuacucccu gcgggccauu aaaaaguugu ugccucugau gcguguugga     1860

gaguauuggu cagcagauug caucgacagu gaaacaaaag agagaauuag ccaaauaauc     1920

aggggcgaga uggacgaaaa ggugucccag cgcaugacua agucucggga uagcuucacc     1980

gacauuucac aguuucaggg ucuccccguc uggcaagccu guuacguggu auacgggaga     2040

cacagugagg cuacagaaau ccagaggugg gagaaaccag aggauaucga cguuuaucug     2100

aaggcguuca aacagcauuc cuuacgaaac cccauugugg aacaagucgu gcuggagacu     2160

cuucgcgucg ugcgggauau auggaagacc uacggcaaag uugacgaaau ccacauugag     2220

augggaagag auaugaagaa uacagcagaa gagaggaagc guaucuuuaa ccgcaaucag     2280

cagaacgaga augacaacuu gcggauuaaa gcccuccuga ccgaguucau gaauccugau     2340

uuugaaaucg aaggcgugag accauauagc ccgucucagc aagaggcucu aaggauauac     2400

gaagacgggg ccuggucagg acuggaugag gagaaggaag acaaaaagga gcucgaagca     2460

auccgagaga uucuggguaa auucagucag agcgauaagg ccaaacggcc cacucauucc     2520

gaaguacuuc gcuauaaggc uugguuggag cagagauacu cuucgccuua uacgggcgag     2580

gccaucccac uggcaaaguu auucacaccc gcguacgaaa uugagcacgu caucccucag     2640

agcagguuuu ucgacgacuc cuuuucaaac aaagugguuu gcgaagcuga ggugaauaag     2700

cucaaagauc ggaugcuggg guacgaguuc auaaaggccc aucaaggaga agucgugagu     2760

cuuucuggca ccggucgaug ugcacgcauu cugagcccag aggaauauga gcagagagua     2820

acugacugcu acaaacacaa caccgccaag augaagaaau ugcucaugga agagaucccc     2880

gaugacuuua uucagaggca gaugaaugau ucccguuaua ucucaaagcu ggugaaaagc     2940

cuacugucua acaugguucg ggaggaaggg gagagagaag cuauauccaa gcacgucauc     3000

acauguaacg gcaccauuac ugacaggcuc aaaaaggauu ggggagugaa ugacgugugg     3060

aaccauauca uucugccgcg cuuccaacga augaaggauc uuacaggcag aaaugacuuu     3120

gucgccguua acagucaggg gcacgagauc ccuagcgugc cacccgaauu gcagaaagga     3180

uucaagaaaa agcggauaga ucauaggcac cacgcaaugg acgcuauugu gaucgccugc     3240

acgacccgca aucauguaaa ccuguuaaau aacgaggcgg ccaugucuga gaauaaagcu     3300

aaccguaucg cacucuccag aaagcugcgg agguacgaaa auugcgccau uuauaagggu     3360

ggcgagacua aaaacaucuc agucgcuaag gaauuucuua aaccuugggc cacauucccc     3420

gcagacgugg agcgcgcgcu gggggauauu guugugaguu uuaagcaaaa ucuacgaguc     3480

auaaacaaag ccaccaauca cuacgaacau uauaaggacg gaaagaaagu gaagguucca     3540

cagaaaaagg gcgauaugug ggugguaaga aaaccucucc acaaggacac agucuacgcu     3600

cuggugaacu ugcggaacgu gaaaacuguc ggucugaagg augccaucuu aaugcccgac     3660

aggauugcaa auaaggaucu caaaaagaaa guuaaggagc ugcuugcuca gggggacgau     3720

cugaaaaaga ucaagaaaua cuucgagacc gaaaaggaca cguggcagga uguggacgug     3780

aaaaagauug agguauauua cuuuagcgaa gagaccgcca accauuauua cgcaacacgc     3840

aaaccauucg augccaaugu caagaucgac ucggugacug acaccggcau acaaaagauc     3900

uugagaaggc accucgagga aaacggaaau aaagcugaug uugcguucuc cccggagggc     3960

auugcccgga ugaacgaaaa uaucuuucgu cugaacggga auaagcacca ucagccuauu     4020

uauaaagugc gcgucuacga gaaggcaaac aaauaucccg ugggacagac aggcaauaag     4080

gcuucuaagu ucguugaagc cgccaaaggu acuaaccuau accuggcugu guacgagagc     4140

gaggucguag acaagaaaac cgcagaaauc ucaaagaaac gaaguuaugu gauaguucca     4200

cuuagagaug ccguggcgag gcagaagcaa gggguccccg uggcuccuga gcggaaugaa     4260

gagggaggca aacuccuguu uguauugucc ccaaaggacu ucguguacgu ccccacggaa     4320

gaggagcgcu cuaaggguga aguucuguua ccucucgaua aaaacagaau uuauaucaug     4380

auggacgcca gcgauggcau cgugaauuuu cugccguacu ccguggcaaa ccuuauuuau     4440

gcccugccaa aggaccaggc uucaggguuc uguacagaug gaaggaugau acagaacgag     4500

uuuggcuugg ggagccccaa aaguaagaau gaacgggcac ucaccggaga gaugaucaaa     4560

gaaauuugcc ugccugucaa gguggaccga cuaggcagaa ucacugagau uaacgguaau     4620

aagguuuga                                                             4629


<210>  3453
<211>  4644
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3453
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccugacaaau       60

aacgauuucg acaauaaguu ggguuccaua gagggcuuag ggucuagaau cauucccaug      120

ucacaggaug ugcugggaaa auuugacagu ggcgucagca ucucccaaac ugcugaaagg      180

acgaaguacc gggguacccg ccgacugguu cagcguuucc uccugagaag ggagcggcuu      240

caccgcauuc ugaacaucuu ggauuuucuc ccuagacauu auucugaagc aauagacuuc      300

gauaaaaagu uugggcaguu caagccagag agggaagaga aacugucguu uuacaagaau      360

gagucaggga aaaaccaguu ccuguuucuc gaaagccacc aagagauguu cagugaauuu      420

aaguuaacac auccggaccu gaaacaggug cccuaugauu ggacuaucua cuaucuucgg      480

aagaaggccu ugacccagaa aauuuccaag gaggagcugg cguggaucau ucuaaauuuc      540

aaccagaaac gcggcuacua ucaacuccga ggcgaagagg aagagguaaa uaagaacaaa      600

cuggaagagu uucacacacu ugugaucguc gacgugauag agagagaaaa ggggaaaucu      660

ggaguuuggu acaaugugau ucuggagaac gguuggaucu acaagaggga aagcaagauu      720

gcuuuguucg auuggaucgg caaacagaag aauuucaucg ucaccacuga gcucaacgac      780

gaugggacaa uuaaacugaa uaaggacaac gaagagaaac gguccuuucg uucaccuaag      840

gaggacgauu ggggauuacg caagauaaga accgaacaga cgaucaacca gagugagaaa      900

agcgugggcu cuuucauuua ugacgaacug cucaagaaac caaaucaaaa gaucaaaggu      960

gagcuuguaa ggacuauaga acggaaguuu uacaaggaug agcuguccaa aauuuugaag     1020

gcccagauca ccuuccauua ugagauccua ggggaccgaa acauuuacaa aaagugcauc     1080

gaugaacugu aucgccacaa ugacucacac aaaaacaaua uuaaggauaa gggauuugac     1140

uaccucuucc uggaugacau aaucuuuuau cagagacccc uuaaaagcaa gacaagucug     1200

auuucugauu guuccuugga guucguuccu aucaaagaca agaaaggcau ccucgugaag     1260

gacagcaacg gcaaacaagu cgugaagcca cugaagugca uugcaaaauc gaaucccaua     1320

uuucaggagu ucagguuacu gcaguuugug aagaaccuaa aaaucuacaa gaaagaaauu     1380

aucaaugaga uugaugucac uaacgacuac cucaagaccg aagaggaucu gguugaacuu     1440

uucgacuggu ugaaugauaa ggccgagauc ucucagaaac aauuucuggc uuauccuaag     1500

uucaaacuca aggaagacaa auaccggugg aacuucgugg aggauaagau auauccaugu     1560

aaugagacac gcaaccaguu ucugaaugca aucgccaaga uugacguaaa caaauccuuc     1620

uuugaugcua agaacaccca ggaccuuugg cauauccugu acucagugac ggauaaaacu     1680

gaauuguuca guauuaauac agagaccggg auccagagcg gagcccucuc cacuuuugcg     1740

aagagacaca accugucuaa ugaguucguc agcaacuuuu caaaaauaaa gccguauaau     1800

aaggacuacg guaguuauuc cgaaaaagca auuaagaaau uacugccccu aaugagguuc     1860

ggcagcaacu ggaauaagaa caauaucgau gccaaaacac ucgcucguau ugacaagcug     1920

caaaccgggg aauuugacga gacgaugcgg acccgaguga gagagaaagc cauccaucuu     1980

ucuaagauaa cagauuucca gggauugccu cuguggcucg caucauacau cauuuacgac     2040

aggcacagug aagcuagcaa caucuccaag uggaaaacuc cagaggauau ugccugguuu     2100

cugaauccca agaacaaauc uggcuucaag cagcacucgu uacgcaaucc uaucguugaa     2160

caggugauaa ccgagacacu gcgggucgug aaagacauuu ggcaacauua uggugaaggg     2220

aaggagaacu uuuucaauga gauccacauc gaacuuggca gagagaugaa gaacaacgcg     2280

aaugauagga aacgcauuac ugaaaccauc aaccagaaug agaacacaaa uuugcgaauu     2340

aaggccauac ucaccgagcu gaaaaacgac ggaaucaaug auguacggcc auacagcccc     2400

ucccagcagg aaauucuaaa gaucuaugag gaaggcguuu acaacucaga gauucguaaa     2460

gaaaagcugg aggacaucga uaagauaagg aaaaaugcaa agccgacucc uagugagauc     2520

auuaaauaua agcucuggcu ggaacaaggg uacaucucuc ccuauacggg aaaacuuauu     2580

ccauugagcg accuguucac aaccaaguac cagaucgagc auauaauucc ucaguccaga     2640

uuauuugaug acucacucag caacaaggug aucugcgaau cugaggucaa ugaucugaaa     2700

gguaacaaua cugcucuuga guucauccug aagaacgaag gccgcauugu guugcucacc     2760

gggggaaaaa cagucaagau auuuaccaaa gaggccuaca aucaacacau cacuuccuau     2820

uacauuaaga acaagaguaa acagaagcgg cugcuaacag aagagauccc cgaaaaauuc     2880

auugacagac agcugaacga cacgaaguau aucagcaaaa uagugaagaa ucuccugucu     2940

aaaaucguua gggaggaaga ugagcaggag gugaccucca agcauauugu aucacuuaac     3000

ggcaguauca cuagccgaau gaagcaagac ugggguuuga augaugucug gaacaaaauu     3060

gugucgccac gcuuugaacg gcugaaugcu aucacaaacu ccaaugacuu cgggaaguua     3120

gagcucaaaa aggaugaauu uggcaacgcc ggaaaacagg uuuuccagac cacagugccc     3180

gacgauauag caaagggcuu uucuaagaaa agaauugacc acaggcacca ugcccuggau     3240

gcucuuguca ucgcguguau caaucguacu cacauuaacu accugaauaa ccuaaaugca     3300

aaggacgaga acgauaaaac caucaagcau gagcuccgca auaaacugug cuucaagacg     3360

aagccugaca gcaacggaaa uucaaaaugg gaauuucaca agccauggga gaacuucacc     3420

aaaaaggugg aagacgaguu gaaccagaca auaauuaguu ucaaacaaaa uacucgggua     3480

aucaacaaga ccgugaauaa guaucagguu uggaaagaag aggauggagc caugaagaaa     3540

gucacaguga agcagacuaa aggugacaac ugggcuauua gaaagccgau ccacgccgag     3600

accguguccg gcaaagucuu ucugaagagg auaaagcagu cucauauuac gaucgcaaau     3660

gccaucgaac aaauugaguu aaucguugau aaagaagugc gaaagcagcu caaagcuaag     3720

auuaaacagu accccaacaa uaaggagggg uucaagaaac accugaaggc cuuuccuaua     3780

augaucaacg gaaaagcaau ugacaaggug caaaucuaug aaacaaucga ggcgaccgcu     3840

acucgggagc gcaauaaccu ugauauuaca uucgacgaga aaaagauaga aaagaucacc     3900

gauacuggca uucagaaaau ccuguugaau caucucaagc aggaaauuua ccagaacgcc     3960

aucgacgaga gcgguaaaaa gauagcacca cacgaaguag ccuuuuccga gaucgggauu     4020

gaugagcuga auaaaaacau caagguccua aauaacggca agaaccauca acccauuaaa     4080

aaggugagaa ucuucgaaga gucaaaaagg uuuagccugg gacagagugg cucuaagacc     4140

gacaaauacg uugaaacagc uaaggggacg aaucuuuucu augccauaua cgagggcaaa     4200

aagggaaacc ggaauuauaa gaccauuccu cucaacgagg ugaucgaacg acagaaacag     4260

ggucuaaauu ccgcauuggu cacugaugag aacgaaaaua gacuguuauu uacacucagc     4320

ccaaacgacc ugguguacau ucccaccaau gaggaaaaga aaaaccuuac aacuaucaau     4380

uucgauaagc ugucgcgcga gcaaaccgag aggauauaua aauuuguuuc auguacgucu     4440

ggggaaggac acuucgugag uaacaauuac uccaaggaga ucauuagcaa cgaaaauggc     4500

ucuaacaaca agaaugagcg uauguuggaa uuuaacaaua gcaacacaau cuaugacgag     4560

aaagagaagc cgguaaugau uaaauccguc ugcuggaagc ucgaagugga ccggcugggu     4620

aaaaucaaga gaauaauuag guga                                            4644


<210>  3454
<211>  4677
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3454
augaagaaaa uccugggccu cgaccuuggg accaacagca uuggaugggc ccuggugaau       60

gucgauaaga acgguaaucc cuucggcaua gagggguugg gauccagaau cauuccuaug      120

gaccaggcua cauuaucuga uuuugaaaaa ggcaacucaa agagucaaac ugcagagagg      180

acgauguucc ggggugcccg ccgacugcgu gaaagaaaac accuaaggcg ggagcgccuc      240

cauagaaucc ugaaugaacu uaacuuucug ccaauucacu acaggagcug caucgacuuc      300

guucaucggu uggggaaguu uaaggcgaau cacgagccga aaauagcuug gcgcuccaac      360

gaucaggaca agauggaguu caucuuucaa aauucuuucc ucgaaaugau gucggauuuu      420

accaaacgac agcccgagcu ggugaaggaa ggcaaaaaga uuccuuauga cuggacaauc      480

uacuuccuga gaaagaaagc ccucggacaa aggauugaga aggaggaauu agcauggcug      540

cuuuugcauu uuaaccagaa acggggcuau uaccagcugc guggggagga ugaaaucgac      600

gccacucgcu cagcuaagac cagaaaauau uucgauagga agauaauuaa aagcaucauu      660

gacacaggag aggaauacaa gggucuaaag guacucaucg uggagcugga uaauggcaac      720

caggggaaaa ucuuuaagca agagguccca gacugggugg gacaggagaa aaauauuaua      780

gcaaccguug accuugauaa ggacggcaaa gaucgguucg acaagggugu gcugagucag      840

cgauucucca uccccucuga uagcgaaugg gaaacugagu ggaaguugau uaaacucaag      900

acagaagagg accuggaggc cucccagaaa accgucggga cguauaucua cgauucauua      960

cugaagaacc cuaaucagaa aauaaaggga aagcucgugc gcacuauuga aagaaaauac     1020

uauagggacg agcuucgggc uauccugaac aagcagcgcg aauuucaccc agaguugaac     1080

gauaaaaagc uauaccagca cuguaucuuc agucuguauc cccaaaauga agccuuuaga     1140

aacagcauug cgaauaaaga cuucaccuac cucuuucugg aggauaucau uuucuaucag     1200

aggccucuua agucuaagaa auccacaaua aacaauugcg cauacgaggu acgucgguau     1260

auggacaagg aaacuaaaga gcugaaggaa guuccauuga aauguaucgc caagucacau     1320

ccgauuuuuc aggaguuccg acucuggcag uuuaucagca accugagaau cuacaaaaag     1380

gacauuaccg auauagacgu gacaaaugau uucuuacaca gucaagacga uuacgcuaac     1440

cuguuugagu ggcuaaauaa caagaaugaa aucgaccaga aagccuuccu caaguauccc     1500

gcauuuggcu ucaaaaagga ugcugucaac uacaggugga auuaugugga ggacaaaucu     1560

uacccuugca acaagacccg cuccgccauu cugagcaaac uugauaaaca ugaacaggag     1620

uucuugacga aggaacugga gcucaaaauc uggcagcugc uuuauucggu uaauucuaag     1680

gaggaaauug acaaagcgcu gaacgucaac uccccaaaga ucaauaacaa gauaucaauc     1740

gaucacacug gcauuuugca cgagcucaaa aguagcuccc ugucugacau cagcauucaa     1800

cgguuaaagg ccaucaauuu ugaagagaaa gauuacgggu cauauaguua caaggcaauu     1860

aaaaagcugc uagcucucau gagacaggga aaguacuucg acaacacagc cauaaccgac     1920

gcuacuaaag ccaggaucaa uuccauuuuu gaucgccugg aaagcaucuc uuucgacccc     1980

gagcgaauaa acucaaucag ugaugacgau auuccuaagc agauccuuaa gagcuuuauu     2040

aaguugaauc ggacauaugg uggcuucaac accuaccagg cauguuaugc cguuuacggg     2100

cgucauuccg aggcuaaaga aaucgcgaag ugggagaaac cagaagacau agaucaauau     2160

cuggcaaagu uuaagcagca cucucucaga aaucccauug uggagcagau caucacggaa     2220

acccugaggg uagugcgcga cauuuggaaa aagcaugccu acaaaacaau caacaaggau     2280

aaucagggaa acaauugcga gucggagagc gaauuaacug agauucacau agaacugggc     2340

cgggacauga aaaacaauaa guccgagaga cuuaggaucu cacgaauuaa cagugagaac     2400

gaaaauacca acuugcgcau ccggaagcuc cugauugagu ucaaugaucc ggaauuuggu     2460

gucgagaacg ugagaccuca uucucccagc caacaggaaa uccuaaggau auaugaggac     2520

gggguucuga aauccggcgu ggagauccca gaauacgucg auaauauuau caagaaauuc     2580

aaggagacag acgugaaaaa gcagccuuca aagagcgaag uacuccguua caaacugugg     2640

cuugagcaaa aguaucgcuc ucccuacacu aacgaaguua uuccauuggg aaaacuguuu     2700

accaaugacu aucagaucga gcacauaauu ccccaguccc gguacuucga ugacaguuuu     2760

agcaacaagg ugaucuguga ggcugaaguc aaucaguuaa aagagaacga acucggcugc     2820

gaguucauca ugaauaacgg gggaagaauu guggaacugu cuuucgguaa gacagucagg     2880

auacuugauu uugaggccua ugagcaauuc gugcagcaga auuacgcagg caaccgaccu     2940

aagcugcgga auuugcucau gaccgaaauc ccaaacgacu uuauugaucg ccagcugaac     3000

gacuccagau auaucucaaa aguugugaag gggcuacuga guaauauugu aagggaggau     3060

ggagaagccg agagcacuuc gaaaaaccuc aucuccugua cgggcggugu cacagcucgg     3120

cugaagaugg acugggggcu uaaugaugug uggaacaauu ugauauaccc gcgauucgag     3180

agacugaacg aauuaaccaa uucuaaccaa uuuggcuacu gggacaaaaa ggauggaaag     3240

aauguuuucc agacucaggu gcccauggac auccagaaag gcuuuaacaa gaaacgcauu     3300

gaccacaggc aucacgcccu cgaugcacug gucaucgcgu gcgcuacccg uagccauauu     3360

aauuaucuua acaaugaguc agccaagagu aacuccaaag aaucucggua cgaccugaga     3420

aggaagcuac gccgacucga ggugauccaa aaagaauaua caauaaaugg gagaaaggag     3480

auucggaaca ucgagguagc aaaggaguuc uacaaaccuu ggaauagcuu uacccaggau     3540

gcccaguccg cucuggaaaa caucgugguu ucauucaagc aaaacuugag ggucauuaau     3600

aaaacuacaa acuauuuucu gcgcuucaau aaggaaggua aaaagaucuu cgagacgcag     3660

accagcggaa guaacugggc cauacguaag ccauuacaca aagacacugc auuuggccuc     3720

gugaaucuga agagaacaaa aaccgugucu cuugcuaacg ccauucugga uuacgaccag     3780

aucgucaauc ggaaguugug gacaaaaauu aaggaacuca ucuccauagg guaugauaac     3840

aagaggauuc ugaaauacuu cacugacgau aaggagaauu ggaacgagau caucagcucg     3900

ggaauugaag uguuuuacua uaccgacaau uucgagaaac agguggcgac gcgcaagccc     3960

cuagauaccu cauuuacaga aucuaaaauc auuaacagua uaacugaccu gggcauccac     4020

aagauucuuc ucgcccaucu gcgagcuaau gaggguaacc cuaauaucgc auucucccca     4080

gaagggaucg agaccaugaa cagacacauu ugugaguuga acggcggaaa guuucaucac     4140

cccauauaca aagugcgggu acaugaaccg cugggcuaua aguucaaagu cggggauaag     4200

ggaaauagga gcacaaaaua cguggaggcc gcuaagggca cuaacuuauu uuucgguauc     4260

uauauuaauu cucaagggau ccgcagcuac gaaaccauuc cucucaacgu ugccaucgag     4320

agacagaaac agggacuguc cccagugccc gacaauaacg agcuuggcga aaggcuguug     4380

uuuuggcucu caccuaauga ccuggucuau cuacccacga gugaugaggu ggaaagcggu     4440

auaugcugca acaagcuggg caucgaccgg auuuacaaga ugguuucuug uacaggguac     4500

gugggagauu ucaucccagc acacauugcg aauccuauca uacagacccu ugagcucggc     4560

uccaacaaua aagcccaacg ugcuuggacu ggggaaauga uuaaggagau cugcauuccc     4620

guaaaagucg accgcauggg aaacaucgug gagauauuua aucgaaacaa gaauuga        4677


<210>  3455
<211>  4695
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3455
auggccaaga uccugggccu cgaccuuggg accaacagca uuggaugggc uauaguggau       60

gacaaaaauu ccaagauccu ggauacaggu gucagaauuu uccccgaggg cguuguggca      120

aaaacuaucg ggucuggaga caaggaagag aguaaaaacg ccaagaggcg ggaaucacgc      180

cagacgcgac guggcuuuua cagaaagagg uugcggaaaa uuaaguuacu gcgcacccua      240

aucaauuuca aaaugugccc ucucgagcac uuugaacugg auguauggag caagugggac      300

aaaauaaaga agaaagaggg uagaauggcg ccagagcuuu uccugccgga aaacuccgcu      360

uauaauuugu ggcucaagca aaaccccuac auccugaggg auaaagcccu gucuaaggac      420

cucacauuaa cugaguuugg gcgggugcug uaucaucuua uucagcgccg aggauucuug      480

ucgaauagaa aaggcaagga ugacggcaaa aucuacgggg gaaaggaagg uauugucggc      540

aucgauaaga ccaacgagca gauuaaaaau ucaacacugg ggaaguuucu auucaaaaua      600

cucccuaagg agggagaacc auuuaaaauc auugaggacg aacugggcaa cgagcuuagg      660

gugcggagcc guuauacccu gcgcgauaug uacaucgcag aguucgaagc caucuggaau      720

agacaggcua agcacuuggg ucucgacaac auuuauuaca guaauguuaa gaacgugcug      780

uuaaaagggu cccugaagaa uaaaaagaac gagaggaaaa uaaaccaucu caagaagauc      840

aaaggaaaug gcaacauuga aaucauaacu cuuaauaaca aucugucuaa gaacgagaaa      900

cccaaguacu ugcuaaaaau ugcaacaaag cugccucuca aggaauuucu ggccggccgg      960

aucgagaaag acgaagaugg gaagaucaaa uucaagagca augaguccgu cuuguuuugg     1020

caacgaccac ugcgcucaca gaaaaaguug cucgcuaaau guagauucga gcccgaccug     1080

aaggaugaca acggaaaguu cauucagaaa gguaagaccc cuugccacuu aagucaucca     1140

cuguaugaag aguacagggc cuaucaguac gugaauaaca ucgaauaugg caaaaagcaa     1200

cggcuagagg aaagccagcg caaacaggua auugaucuca uaaauucuaa ggacgggaac     1260

uuuaaguucu ccgagaucaa aaagaaacug aaacuuucau acgagacguu uaauuauagc     1320

gaugacaguu cuguuaacgg aaauuacaug auuaaaaacu ugaacuccau cuucccgaag     1380

gaaauccugg agaaggaauu uguggucaau gugaacaaag aggauauuau agaguacggc     1440

uaugaaagaa ucuggcaccu cuuccacuuu ugugaggaca augaugugcu gauuaagaaa     1500

cuucaggaca acuucgaccu gagcgaaaau gauaucgaga agauuucgaa aaucaacuug     1560

aaggaagguu acucuaaugu cucccucaag gcgaucaaaa acauacugcc cuauuuaaag     1620

gaggggaaac ugcuaucaga cgcaguuauu cucggcggag ugaggaaugu auuuggcaag     1680

aaauggcaug aguucaucga uuuucacgaa aagauugaga aagacauccu guacauaaac     1740

caucaaaaga guaauaagga aggggaggcc auugagaaaa ucaagaaaua ucuuauugau     1800

uacaaguucg gauuuacuga aaacgacaau cguuucaaaa aguuguauca ccacagccag     1860

gauguggucg aggugaagcu gcagaaaaag aucgguccug uugaagaccu ccggaaccca     1920

auagugcaac agggcgucaa ugaggugcga agacugguaa acgaauuacu ggauguucau     1980

gggaaauucg accaggugag ggucgagcuu ggacgcgauu ugaagaacaa uaaauccaag     2040

cgggaagaga ucggcuacaa gauuagagag aacaccgcua aaaaugaaaa ggccaaagag     2100

cuccugaucg aauacggucu aaggcagucu cgcgacaacg ugcaaaaggu ccugcucuuu     2160

caggagaugc uggaucgagg gacaguggca guuugccccu auacuaauaa aaccauuaac     2220

aucagcgacg ugcuuggcaa ugagaacaag augcagauag aacacauuau cccuaaguca     2280

aucaguuugg acgauuccuu cgcuaauaaa acacugugug acagcaaguu uaacggauua     2340

aaaggcgagc ggaccccaua cgaguucuau cagauuaaua acgaugccaa ucucugggga     2400

gggucuaagu caugggaaga aaucgagcgu agaacguuua ggauucugcc cuacaguaaa     2460

gcgaagcgcu ucacuagcaa gacaaaauuu gagaccaacg acuucauaca acggcagcuu     2520

aaugauacua gauauaucuc caagaaaaca aaggaaauuc ugucucagau cugcgaggac     2580

guaaggguca ugccggguuc guugaccgcc gaacuccgaa aacugugggg ccuaaacaac     2640

auucugcauc cugcauuugu gcucgaucug cccgcccuuc acaucgacgc uaauaagcau     2700

auaccacacu acguuguguu ggaugaggac aacgucccua uccugagcca gcgcauuuau     2760

aaugauaaac ccaucuuaaa ggcuaaccaa acguccauua ccggguucgu ggacaaugaa     2820

aucuuuaagu caaaagagaa guacguaaaa uucgagguga aggccccaaa ccucaguaau     2880

ggagaauacu gggcaaaacu gcuucugucu aaccccaaga gcauaguucg ggucuuuaag     2940

gagaaaccua aguccacaga aaaugagauu guggugagag gcaaaaucga aaaggagaaa     3000

uucaagcacg agggucuauc agggaucaac gccgaaggca aggagaaugg aacuuauugg     3060

gcuaaacuca gcauugucga uaagaaauuc aagaaaccaa agaaggacga ccagccgaaa     3120

aagaacggca aacagauacu guuguuuggg gcgguuaagg aaggaguguu cucuuccuac     3180

auguaugagu gugaugcaga ccuggaggau ggcaaauacu gguuaauccu cgacauugau     3240

uuugaaaaug uggaguucga aaaggcccug aacgagaggc ccgaaguacu ugagaaucag     3300

auccugauuc aagguaucac caacgacgag aaugucuuug ugagugauau ggacugccag     3360

caucaguucu ugaugaacga agggaaagga aaguauuacg uucucuuuga uauaaucagc     3420

gaggugaacg aguucuaucc uauuaagaca aaaucuccag aacuggaaaa uggccaaucc     3480

cuaacugagg guaccaucug ggucgacaag uacacaggcg agauuaaauu ugaucccaag     3540

aaaaaccgug aagaccaccg ccaucacgcu aucgacgccc uggugauagc acuuaccaag     3600

gagacuuacu uccagcagcu cucacggaga aaugccagua gcgaaaacaa gaaaaggggg     3660

aaugaguaug aaaaggagca gcuggauuuu gcugagccuu ggaaagaguu ccacguugac     3720

gcccaaaagu cgauuaacuc caucuugauc ucucauaaac agaauaagaa cauucugacg     3780

aagaucacaa agaagauaac caaaaaugga aagacuuaca aaagcgaagg caugucagug     3840

cgagggcagu uacacgauaa gaccuauuac ggacauaaca agaauuguaa agcagacgaa     3900

uauguauacc gggucgauau uaacaaucuc aguuucuccc agcugacaaa caucauugac     3960

cuuaaucuga aggcggugau cguggagggc gucauuaaaa aguuugagga aucugagaaa     4020

cuucucuuca agaaaaugaa cagcaagguu caaaaccugg auaaguccaa agugaagaaa     4080

auaaaggcua aaguagacaa gauccuaaag gaaaccucau uuuucaugcc aaaugagggu     4140

aaacgcuaua ucagacugaa cauuaucgaa cccgccaauu uugagaggau gccggugccu     4200

auuaagaaag uccggauacg aaaggugcuu agaaacgcag agaaacucaa uaacgaaauc     4260

aagcaguucg uugauccaca gaauaaccac caugugcuga uuuacaagga cgagaauggg     4320

aacuugaaag aagaggucgu gacuuuuugg acaguugugg aacgcaagag gacgggagag     4380

cccgucuaca aauuaccuag cgauggcaag aaaaucguaa ccacucugca gaucaaugac     4440

auguuccucc ugggucuuaa cgaggaugaa auuaauuggg agaaccccaa uuauaaccac     4500

cugaaggaac acuuguaccg ugugcaaaag uuuacaagug gcgacuucua uuuucggaua     4560

gccaaaaccu cuuccaucca gaacggggag gaaaagcagc aaauuaauag cuucggacua     4620

ggcaaaaacg gguggcugac acauaaucca aucaagguua aaauuucggu gucaggaaag     4680

auccucaagg ucuga                                                      4695


<210>  3456
<211>  4713
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3456
auggagaaca gcaauaacuc cucuaagaaa uacuaucugg gccucgacau ggggaccgau       60

ucaugcggau ggugugugac agacgauaag uacaauauca uuagaaguca caaaaagaua      120

uauggucccg acggcaaacu ugucaagagg gaagggugcc aucugugggg aagccgguug      180

uuccgcccug ccaacacugc uaagucccga cguuuaaaua gaacggcaag gcggcgccug      240

gccagaaggc ggcgccgaau cauucuacuc agagagcugu uucagccaga aaucgauaaa      300

guugacccga acuucuuuag gcggcuugau caaucuaauc uguggaacga ggacaaggau      360

aaagaauugc agauggagca caauuuccuc uuuaacgaca aggaucugac cgacaaagag      420

uacuauaagg auuuccccac aauuuaccau cugcguaagg cgauggugaa uggccucacu      480

aucaacgguu auuacuacga cccuaaaaag caguuugaca uacgcgaagu auauuuagug      540

cuggcucaca ugaucaaaua cagagggaau uuccuuaugg agggagaugu caaggacucg      600

gugguugauc caaccuauuu gaaacagugu uuugacaaca uugaugugau ccugauuuca      660

cuaaauaacg gcgaagacga ugagaacgag gacgauaaga ucgaccccag cgaaugcugu      720

uucaaugucg augagaaaaa ggccaggaag cucgcaaaca auuuucaaga aacaaacaaa      780

gugagugacc uggaggacga ugaacgggac cuuuucggca aguuuaauuc caaagauaua      840

cgaaagaaaa uucugaaguu gaucaacggg ggaucuggug uaaagcucaa agacuuccug      900

ccuaaggaga uuuuaguugc ccaugauggc gacgaugagu acggggacaa aaagaucgau      960

cugagcuccg aaacccucga ggacgauauc aauggauauu cacuuccaga aaguuacgug     1020

gagauuauac uggaagcuaa aaagaucuau gacuucaaga cuuuggcaua ccuacugcac     1080

ggcaaaagcc uccugugcga ugccaugguc aacguguacg aggagcacaa ggaacagcuu     1140

gcugcccuga aacgcuugua uaagaaauac gcgacaaagg agcaguauua cuuuauguuc     1200

agagacucua ggauucucga cggugaugac uccgaugacg aagaggauaa ggaagaguca     1260

aauaaagagg aaagcaagga caaaaagaaa aaggcaaguu augugucuua caucggguau     1320

accaaaacga acuccaaggc caaguacacu ggagcucaua uaaccagcga gaaaaauauu     1380

ucggcccuga ucaagcagau caaagaugac uuaaaguuug augaccugaa aucuaagguc     1440

gaugaccccu cccaacagaa cgaacuauca gaugaggaca agcucgacau ucaguguauc     1500

gaggaagcaa uugagaguaa uacauuccug ccucggcaga acagccgcaa uaacggcgcu     1560

uuuccauacc aacuuaacuu gaaugaaaug agaacuauaa ucgagaacca guccaaauau     1620

uacccguucc ugaaggauaa agcccccucu uuuccugacg gcaagaccga agaguauaaa     1680

auuguuagcc uccugaaguu caagauccca uacuauaucg ggccccuuuc acagccuaau     1740

gauuacguga gggacgauaa acgugacaac cacuggguac aauuucgggg agagaauaac     1800

caggguauua uaaauccaug gaacuucagu cagaucgugg aucuggaagg cacagcggac     1860

aaguuuauuu ccaauuugac caacuauugc acguacaucu ucggggagaa uacucucccc     1920

aaagaaagcc ugauuuuuca ggccuacaag gucuuaaacg aucugaauaa ccuaaaagug     1980

aauggaucuc ucaucucaaa ggaggacaag gauuauauaa uccaaaacgu uuaccugaaa     2040

aagaaaaaga uuaaaggcaa ggacaucgug aacgcacuua aaaauaagua uaaguugagu     2100

gacgaagauc cgacagucac cacuaaaggu agcgggaagu ccauuggcga ggacgaggug     2160

gaucugacaa uguucaacuc uucgcucagc uccuucaucg acaugucaga uccuaaugcu     2220

uuuggacccg aguucuacaa agacaguaac aaguuugaac uggccgaaaa aguaauagag     2280

uuaauuacca ucguugaaga uaaggaccug cuuaaggagc gauugaaaaa gcucggccug     2340

ucugauaauc aggugaacua ucuauucaaa cagaaguaca gcgggugggc uccacugucc     2400

agaaaacucc uggacggaau uacgaucgau ugugucaaug agaacaccgg ugaagccuau     2460

ccugugacaa uaaucgacgu caugagggau acuccccuua auuuuaugga gauuuacgaa     2520

accaaggagu cacaguucag cggcuuuaag gagaaagugg cagaauugaa cgacgagaag     2580

aaacugucuc gcgacgauuu aaucgaagag uccuacgcca guccagcuau gaagcggagc     2640

guuagacaaa cauucaaagu gauugacgaa cucaagaaua cucugaacau cgaucauuuu     2700

gacaggauau ucguagaggc gacccgcgca cccggggagg ccggaaagau ggucaaaucu     2760

cgaaagaaac agcuucagga ggcuuaugau gccgcaaagg accuggugga ucaggaaucc     2820

cgggacgauu ugcaaaaacu ccgugacgau cuggaaaagc agacagacga ucagcuaaga     2880

aggaagaaag uuuaccugua uuuuaugcag cucggccgcu caguguacag uggugagaag     2940

auugaccuga augaacuuga caaaaacuau gauaucgacc acaucauucc ucaagccaag     3000

uugaaagaug acagcuucaa uaacaugguc cugguggagc gggaguuaaa caauaagaaa     3060

caggaugaau acccaauccc gucgaccauu auauccgagc acgggagaaa cuggauccag     3120

aagcucgcua agauucaaga caaaaauaag aggucuuauu uuaugcccaa agauaaggcc     3180

aaccgacugc uucgcccuga aagcaaacag cugacuucag acgagcuaaa uggcuucauc     3240

aaccggcagc ucacgcugac aaaucagagu guaaaggcag ugugcgauau uuugaagaug     3300

accgaaaaag gauccgagau cguuuacucu aaggcgaacu ucgucagcga cuuuagaaaa     3360

cuguguggcg aguuaaggcc auccuacgau gcuuucguga aggugcguga cauaaaugac     3420

uuucaucacg ccaacgaugc auaucucaau aucgucguug ggaacgugua cuaugaaaaa     3480

uucaagaauu uuuacauuaa caaucgcauc gacuaugaua acaauggaaa gaaagaccug     3540

ucagugcuug ccagcaagcu guucacuaaa acccaagcug augacaagac agguaacauu     3600

uacugggagg cccgguuuca uaacgaaaaa gagaccgaua guaauggcaa ggagagaacu     3660

aucaagguaa gggacgaagg gacagagucu acguucgaua aaauagaaua uuacauggag     3720

uugaacgacc cccucgucac ccagaugcag uacacucugu ccggaaagca gggcuuuuuc     3780

aaaaagauua cacuaaaaac caaggcaaaa gauaaugaag acaacagcga uaagcuguuu     3840

ccucuuaagc aauaucuccg aucggacuca aauaaaguga uggacguuaa caaguacggu     3900

ggguauucug auaugaucac accauacuuc augcuggugc agagugaggg caaaaaggga     3960

aaacacaucu auuccuugga gagcauuccc acuauauacc ugaagucuuu caagaaaaag     4020

gaagaggaca ucauuaauua uuuaagcugc aaaaacaagc ucaaaaaucc ggucgugauc     4080

uccgauaagc ugcuuaugcg gaccauuguu aagaucccuu acuuuaaaaa cgacucaaau     4140

cugaguuugg ugaagcucgg cacgagcggg cgcaccggaa acaauauaau cuacauuaac     4200

cugucugaac uaauccugcc aauugagugg aaaaauuauu acagaaagau cuccaaccuu     4260

cucggcacaa acguaucaag uacuaaaccc aaggaucuga gcaaguucac cgaacagaug     4320

aaaaaugacc cuacauccac uuuggucgau gguucuaaca agguggagca ugacaaaaau     4380

aacagguuuu ucucguauuu aauaaauaac gugauuaaua agcccaucuu ugaggggcug     4440

ccaaaaacca gcugcucauu caucaagcuc gaauccaaau acgaugaguu uuguagucug     4500

aacacgcuug aacaggcuaa gauuaucgcc acaauaauua agcuguuguc uugcaaaagc     4560

guccaaggau guaagcuauc caaucuguca ggccucccuu cuagccuggg ugcgcuuauc     4620

aguaacaaag ccuuaaccga gggcacuauu aucuccaagg aaagcucuug cgggcugaaa     4680

gagaauauaa uuuucguugu gcccaacgac uga                                  4713


<210>  3457
<211>  5067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3457
auggaguuca agaaagugcu gggccucgac aucgggacca acagcauugg augcgcccuu       60

cuguccuugc ccaagucuau acaggauuac gguaaaggcg ggagauuaga auggcugaca      120

ucaaggguca ucccucuaga cgcugauuau augaaggcau uuauugacgg aaaaaauggc      180

cucccacaag uuaucacucc ggccgguaag cggcgccaga agcgagggag ucguagacug      240

aaacacaggu acaagcuucg gcgcagcaga cugauuaggg uguucaaaac guugaacugg      300

cuccccgagg auuuuccucu ggacaaucca aagcggauca aagaaaccau auccacagag      360

ggaaaguucu cuuuucgcau cucggauuau guacccauuu cagacgaaag cuaccgagag      420

uucuauagag aguuuggcua cccugaaaac gagaucgaac aggugauuga ggagaucaau      480

uucaggcgga agacuaaagg caagaacaaa aauccaauga uaaagcugcu ccccgaagau      540

ugggucgugu auuacuuacg uaaaaaggcg cugauuaaac cuaccacaaa ggaggaacuu      600

auccgcauua ucuacuuguu uaaccagaga aggggguuca agaguucccg gaaagaccug      660

accgagacug cuauccuaga uuaugacgaa uuugccaagc gacucgcaga gaaagagaag      720

uacucugccg aaaauuauga gacaaaauuc guuagcauua ccaaggugaa ggaagucgug      780

gagcugaaaa cggauggacg caaggguaaa aagagauuua aaguaauacu ugaagacucc      840

aggaucgagc cauacgagau ugaacggaag gagaagccgg acugggaagg caaagaguau      900

acuuuccugg uuacccaaaa guuggaaaaa gggaaguuca aacagaacaa gcccgaucuc      960

ccuaaggagg aggacugggc ucuguguaca acugcauuag auaaucgcau gggaucaaaa     1020

cauccaggcg aauuuuucuu ugacgagcug cucaaggccu ucaaagaaaa gagagguuac     1080

aaaaucaggc aguaucccgu gaaccguugg cgguacaaga aagagcuuga guuuauaugg     1140

accaagcagu gccaacugaa uccugaauug aacaaucuaa acauuaacaa ggagauccug     1200

cgaaaacucg cuacaguccu guacccaagu cagagcaagu ucuuugggcc caaaaucaag     1260

gaauucgaga auucugaugu gcuucacauu aucuccgaag acauuauaua uuaccagaga     1320

gaucugaaau cacagaagag cuugaucagu gaguguaggu augagaagcg caaaggaauu     1380

gacggcgaaa ucuacggccu caagugcauc ccgaaaucuu ccccucugua ucaagaguuu     1440

cggauuuggc aggauauaca uaacaucaag gugauuagaa aagaaagcga ggucaauggg     1500

aagaagaaaa ucaacauuga cgaaacccag uuauacauca augagaacau aaaggagaaa     1560

cuguucgaac uauuuaauuc gaaggauucu cucuccgaga aagacauccu ggaacuuauu     1620

ucauugaaca ucauuaauag uggaaucaag auaagcaaga aagaggaaga gacgacucac     1680

aggauuaacc uguucgccaa ucgcaaggag cucaaaggua acgaaacaaa gucccgauau     1740

cggaaaguuu uuaagaaacu gggcuucgac ggggaguaca uccuuaauca ucccucuaag     1800

cugaaccguu uguggcacag cgauuacuca aaugacuaug cggauaagga aaaaaccgag     1860

aagaguauuc ucuccagccu gggauggaaa aacagaaacg gcaaguggga gaaaucuaag     1920

aauuacgacg uguucaacuu accacuggaa guagcaaagg ccaucgcuaa ucuaccuccc     1980

cucaaaaagg aguaugguuc auacagugcc cuggcaauaa ggaaaaugcu uguggucaug     2040

cgcgauggga aguauuggca acacccagac cagaucgcua aagaucagga aaacacuagc     2100

uugaugcugu uugacaagaa ucucauucag cugacaaaca aucaacggaa gguguuaaac     2160

aaauaccugc uuaccuuggc cgagguucag aagagaucca cgcucaucaa acagaagcug     2220

aaugaaauug agcauaaccc cuauaaacua gagcuggugu cugaucagga ccucgaaaag     2280

caaguccuga agucguuccu ugagaaaaag aaugaaagcg auuacuugaa aggccugaag     2340

accuaccagg cgggauauuu aaucuacggc aaacacuccg agaaggacgu gccuauagua     2400

aacucaccag augaacucgg ggaguauauu aggaagaaac ugccgaauaa cagucuucga     2460

aaucccaucg uugagcaggu gauccgcgaa acaauuuuua ucguccggga cguguggaag     2520

ucuuucggaa uuauagacga gauccauauu gaacugggua gagaguugaa aaacaacagc     2580

gaagagagga agaaaacuuc cgagucacag gaaaagaauu uucaagagaa agaacgugcc     2640

cgcaagcucc ugaaggagcu acugaacagc ucuaauuucg agcacuacga ugaaaacggc     2700

aauaaaaucu uuuccaguuu caccgucaac ccuaauccag acagcccccu cgauauugag     2760

aaguuucgga ucuggaaaaa ccagucuggg cugacagacg aagagcuuaa uaagaaauug     2820

aaggaugaaa agauaccuac ugagaucgag gugaaaaagu auauucugug guuaacccag     2880

aaauguagau ccccauacac aggaaagauc auuccccucu caaaacuguu cgacaguaac     2940

guuuaugaaa ucgagcacau aauuccgagg agcaagauga agaaugauuc gaccaacaau     3000

cuugugaucu gcgaacuggg cguaaacaaa gcaaagggug accgacuagc ugccaauuuu     3060

aucuccgagu cuaacgggaa auguaaguuc ggcgaagucg aguacacucu ccugaaauac     3120

ggagauuauu ugcaguacug caaggacacg uuuaaguauc aaaaagcuaa guacaaaaac     3180

cuguuagcca cagagccucc agaagauuuc auugagcggc agauaaauga cacccgcuau     3240

aucggcagaa agcucgcaga acugcuuacu cccgugguua aagauagcaa gaacauuauc     3300

uucaccauug ggucaaucac aagugagcug aaaauaaccu ggggauugaa uggugugugg     3360

aaggacaucc ucaggccucg guuuaagcga cuggaaucca uuaucaacaa aaagcuaauu     3420

uuccaggacg aggaugaccc caauaaauac cauuuugauc ugucuaucaa cccacaacuu     3480

gacaaggagg gccucaaaag acuggaucac cgccaucacg ccuuggacgc uacuauaauu     3540

gcggcaacaa cgagggaaca uguccguuau cugaauagcu uaaacgccgc ugauaaugac     3600

gaggaaaagc gggaguacuu ccucucccug uguaaccaca agaucagaga uuuuaaacuu     3660

ccuugggaga auuucaccuc agaagugaag agcaaacugu ugaguugcgu agugucuuac     3720

aaggagucca aacccauccu cagcgaccca uuuaacaagu aucugaagug ggaauacaaa     3780

aaugggaagu ggcagaaagu uuucgccauu cagaucaaga acgauaggug gaaagcaguc     3840

cgccgaucga uguuuaagga gccgauagga acugugugga uuaagaaaau caaggaagug     3900

ucacuaaaag aggccauuaa gauccaggcu auaugggagg aagucaaaaa ugacccuguu     3960

agaaagaaaa aggagaagua uauuuacgac gauuaugccc aaaaagugau cgcaaagauc     4020

gugcaggaac ugggccuuuc uaguuccaug cggaaacagg acgaugagaa gcucaacaaa     4080

uucauuaaug aagcgaaggu aagcgcuggu gucaacaaga accugaauac aaccaacaaa     4140

acaaucuaca auuuggaggg gagguuuuau gagaagauua aaguggccga auacguuuua     4200

uacaaggcaa aacgcaugcc ccugaacaag aaggaguaua uagaaaaacu cucucugcag     4260

aagauguuca augaccuucc aaacuucauc cuggagaaaa gcauuuugga uaauuacccc     4320

gaaauccuaa aggagcugga guccgacaac aaauauauca uugaaccuca caagaagaau     4380

aacccaguga aucgucuccu gcuugagcau auauuagaau accacaacaa ucccaaagag     4440

gccuuuucaa cugagggccu ggaaaagcuc aacaaaaagg cuaucaauaa aauuggaaag     4500

ccuaucaagu auauuaccag auuggauggc gacaucaacg aggaagagau auuccggggg     4560

gccgucuuug aaacggauaa aggaaguaac guguacuucg uuauguauga gaauaaccaa     4620

accaaggaca gggaguuucu gaaaccgaau ccaagcaucu cugugcucaa ggcaauugaa     4680

cauaaaaaca agaucgauuu cuuugcuccc aaucgccugg gcuucucccg aauuauccuu     4740

ucaccuggug accugguaua cgucccaaca aacgaucagu acguguugau aaaagacaau     4800

aguagcaacg agacuauuau caauugggac gauaacgaau uuauuuccaa uagaaucuau     4860

caggugaaga aguucaccgg gaacucuugu uacuuucuaa aaaaugacau agccucgcug     4920

auccucagcu auucagcguc caacggaguc ggcgaguucg guagucagaa uauuucugaa     4980

uacagcguug augacccccc uauccggauu aaggaugugu gcaucaaaau aaggguagac     5040

cgcuuaggca acgugagacc gcuguga                                         5067


<210>  3458
<211>  3924
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3458
augacccagu ucgagggcuu uacaaaccug uaccaaguga gcaagacucu cagauucgaa       60

cuuauccccc aggggaaaac gcugaagcac auucaggagc agggauuuau agaagaggac      120

aaagccagga augaucauua uaaggaauug aaaccuauca uugaccggau cuacaagacc      180

uaugcugauc aaugcuuaca gcugguccag cuagacuggg agaaccucuc cgcagccauu      240

gauucuuacc gcaaggagaa aacagaagag acucgaaaug cgcugaucga ggaacaggcu      300

accuaucgua acgccauaca cgacuacuuc aucgguagaa cagauaaucu uaccgacgca      360

auuaacaaga ggcaugccga gaucuauaaa ggccuguuua aggcugaauu guucaauggg      420

aaaguucuca agcaacuggg aacugugaca accacggagc acgaaaacgc acugcuccgg      480

ucauuugaua aguucacuac cuacuuuagu ggcuucuacg agaaucgcaa aaacguauuu      540

agcgccgaag acauuuccac agcuauccca cauagaauag ugcaggauaa uuucccgaag      600

uuuaaagaga acugucacau uuucacuagg uuaaucaccg ccguccccuc ucugcgggag      660

cacuucgaaa augugaagaa agcgauuggu aucuuuguuu cgacaucaau cgaggaagug      720

uucagcuuuc cuuucuauaa ccagcuuuug acccagacgc aaauugaccu guacaaucag      780

cuacucgggg gaauaagucg cgaggcaggc acugaaaaga ucaaaggccu gaacgagguc      840

cuuaaccugg ccauucagaa gaaugaugag acagcucaua ucauagccuc cuugccacac      900

cgauuuauuc cccucuucaa gcaaauccug ucugacagaa acacccuuag cuuuauccug      960

gaagaguuca aauccgacga agaggugauu cagucauuuu gcaaguauaa aacucuccuu     1020

aggaaugaaa acguacugga gacagcagag gcuuuguuca augaacuaaa caguaucgau     1080

cugacccaua uuuuuauaag ccacaagaaa cucgagacga ucucuuccgc ccugugugac     1140

cacugggaua cccuucggaa ugcgcuguac gaacgucgca uuucagaguu gacagggaag     1200

aucacuaaga gcgccaaaga gaagguucag agaagucuca aacaugaaga caucaaccug     1260

caggagauua uaucugcagc uggaaaggaa uuauccgagg ccuucaaaca aaagaccagc     1320

gaaauccugu cgcacgcuca ugccgcacua gaucagccuc ucccaacaac ucugaagaaa     1380

caggaggaga aggaaauucu uaaaucucag uuggacuccc ugcucggucu guaucaccuu     1440

cuggauuggu uugccgugga cgagucaaau gaagucgauc ccgaguucag ugcuagguug     1500

accggcauca agcucgaaau ggagccuagc cuguccuucu acaacaaagc gcggaauuau     1560

gcaacaaaga agccauacuc uguggagaaa uuuaaguuaa acuuccaaau gccgacccug     1620

gccagcgggu gggacgugaa uaaagaaaag aacaauggag cuauucuauu ugucaaaaac     1680

ggccucuacu aucuggguau caugcccaag cagaaagggc gauacaaggc ccuuucauuc     1740

gagccuacug aaaagacgag ugagggauuu gauaaaaugu auuacgacua uuucccagac     1800

gcagccaaga ugauacccaa augcuccaca caguugaagg cuguuaccgc ccauuuucag     1860

acucacacca caccuauccu gcucagcaac aauuucauug aaccacugga gaucaccaaa     1920

gagauuuacg auuuaaacaa ucccgaaaag gagccgaaga aauuucaaac ugcauaugcg     1980

aagaaaacag gcgaccagaa gggcuaccgc gaagcucugu guaaauggau cgauuucacg     2040

agagacuuuc uuucuaagua caccaagacu acaucaauag auuugaguag ccucaggccu     2100

uccucucagu auaaagaccu gggggaguac uaugccgagc uaaacccccu gcucuaccac     2160

auuucguucc agcggaucgc agaaaaggag auuauggaug ccguggaaac cggaaaacug     2220

uaucuuuuuc aaaucuacaa uaaggacuuc gcuaaagguc aucacggcaa gccaaacuug     2280

cauacacugu auuggacugg guuauucagc ccugagaauc ucgccaagac cuccauaaaa     2340

cugaacggac aggcagaauu auuuuaccgc cccaagucaa gaaugaaaag gauggcucac     2400

cgucugggcg agaagauguu gaauaaaaag cucaaagauc agaagacgcc aauccccgac     2460

acccuguacc aagagcuaua ugauuacgua aaccaccggc ugagucauga ccucucugau     2520

gaagcccgag cgcugcuucc uaaugugauu acaaaggagg ucagccacga aaucauuaaa     2580

gacagaaggu ucacuuccga caaguuuuuc uuucaugugc caaucaccuu gaacuaucag     2640

gccgcuaauu caccgagcaa auucaaccag cgcguuaaug cauaccugaa ggagcacccc     2700

gaaacaccua uaauugguau cgauagaggg gagcggaacu uaaucuauau uacugugauc     2760

gacucuaccg gcaaaauucu cgagcagagg ucccugaaca cgauacaaca guuugauuac     2820

cagaagaaac uugacaaucg cgaaaaggag cgagucgccg cucggcaggc cuggagugug     2880

guaggaacaa ucaaggaucu gaaacaaggc uaucuaagcc agguuauuca ugaaaucgug     2940

gaccucauga uucacuacca ggcagucgug guccuggaga acuugaauuu cggguuuaag     3000

ucuaaacgua ccggaaucgc ggaaaaggcc guguaccagc aauucgagaa gaugcugaua     3060

gauaaauuaa acugccucgu ucugaaggac uauccagcug agaaaguggg uggcguacuu     3120

aaucccuacc agcugacuga ucaguuuaca uccuucgcca agauggggac ccaaucagga     3180

uucuuguuuu augucccugc accauacacc aguaaaaucg acccccucac uggcuucgug     3240

gauccguuug uuuggaagac aauuaagaac cacgaaagca gaaaacauuu ccuggagggu     3300

uuugacuucc uacacuauga ugugaagacg ggggacuuua uccugcauuu caaaaugaau     3360

aggaaccuuu cguuucagcg cggccucccu ggauucaugc cagcuuggga cauugucuuu     3420

gaaaagaaug agacccaguu cgaugccaaa ggcacucccu uuaucgcagg gaagcggaua     3480

gugccuguaa uugagaacca cagauucaca ggaagguacc gagaccugua ucccgccaau     3540

gaauugaucg cucuguuaga ggaaaagggu aucguguucc gcgauggcuc caacauucuc     3600

ccaaaacugc uugagaauga cgauucucau gcgauagaca ccaugguugc ccugauccgg     3660

agcgucuugc agaugagaaa cucaaaugca gcuacagggg aagauuacau uaacaguccu     3720

gugagggacc ucaacggagu guguuuugau ucccguuucc aaaaucccga guggccaaug     3780

gacgccgaug ccaacggcgc uuaccacauc gcacugaagg gucagcuacu gcuuaaucac     3840

cucaaagagu cuaaggaccu gaaauugcag aacgggauua gcaaucagga cugguuagcc     3900

uauauccaag aacugcgcaa cuga                                            3924


<210>  3459
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3459
augagcaucu accaggaguu cgugaacaag uauucccugu cuaaaacccu cagauuugaa       60

cuuauucccc aaggcaagac acuggagaau auaaaagcca ggggguugau cuuagacgau      120

gaaaagcggg cuaaagacua caagaaggca aaacagauua ucgauaagua ucaccaguuc      180

uuuauugagg aaauccuguc aagugucugc auaagcgagg accuacucca gaacuacucc      240

gauguuuauu ucaaacugaa gaaaucugac gaugacaauc uucaaaagga uuuuaagucg      300

gccaaagaca cuaucaagaa acagauuuca gaguacauca aggauagcga aaaauucaag      360

aaccuguuua aucagaacuu gauugacgcg aaaaagggac aggagaguga ucucauccug      420

uggcugaagc aauccaaaga caaugguaua gaacucuuca aggcuaacuc ugacauuacg      480

gauaucgacg aggccuuaga gauuaucaaa aguuuuaagg gcuggaccac auauuucaaa      540

ggguuucaug aaaaucgcaa gaacguguac uccucaaaug auaucccuac uagcauuaua      600

uaccgaaucg uagacgauaa ccugccaaag uuccuugaga auaaagcaaa guaugagagc      660

uugaaagaca aggccccgga ggcuauuaac uacgaacaga ucaaaaagga ucuggcagag      720

gagcuaaccu uugacauaga uuauaagaca ucugaaguga aucagcgugu cuucucccuc      780

gacgaggugu ucgaaauugc caacuuuaac aauuaccuga accagucagg aaucaccaaa      840

uucaauacua ucauuggcgg uaaguuuguu aacggggaga auacaaaaag aaagggaauc      900

aacgaauaua uuaaucuuua cagccaacag auaaacgaua aaacccugaa gaaguauaaa      960

augagugugu uguucaagca gauccucucu gacacggagu ccaagagcuu ugucauugac     1020

aagcuggagg augacucgga ugugguaacu accaugcaau cuuucuacga acagaucgcu     1080

gccuuuaaaa caguugagga aaaguccauc aaagagacuu uaucacuccu guucgacgau     1140

cuuaaggcgc agaagcugga cuugaguaaa auuuacuuua agaaugauaa aagccuaacc     1200

gaccuguccc agcaaguguu cgaugacuau ucugucauag gcacagcagu gcucgaauac     1260

aucacccagc agauugcccc caagaaccug gauaauccua gcaaaaagga gcaggagcuu     1320

aucgcuaaga aaacggaaaa ggccaaauau cugucauugg agacuauuaa gcucgcacug     1380

gaagaguuua acaaacacag ggacaucgau aagcaauguc gguucgagga aauauuagcu     1440

aauuuugccg cgaucccaau gauuuucgac gagaucgccc agaacaagga caaucuggca     1500

cagauuagua ucaaauacca gaaccaaggc aagaaagauc uacuccaggc uuccgccgaa     1560

gacgauguga aggcuauaaa agaccugcuu gaucagacaa acaauuugcu gcauaagcuc     1620

aagauuuucc acaucagcca gucugaggac aaagccaaca uucuggauaa ggacgaacau     1680

uuuuaucuug ucuucgagga gugcuacuuu gaacuggcaa auaucguucc cuuguauaac     1740

aaaauacgca auuacaucac ccaaaagccu uacucagaug agaaauucaa gcucaacuuu     1800

gaaaauagua cucuggccaa cgggugggac aaaaauaagg agccagauaa cacagcuauu     1860

uuauucauca aggacgauaa auauuaccug ggagugauga auaagaaaaa caauaagauu     1920

uuugacgaca aagcgaucaa ggaaaacaag ggugagggcu auaaaaagau aguauacaaa     1980

cuacuccccg gggcaaauaa gaugcugccu aaaguguucu uuagcgccaa guccauuaag     2040

uucuauaacc caucugagga uauccuuaga aucaggaacc acucgaccca cacgaaaaau     2100

ggaagcccgc agaagggcua cgaaaaauuu gaguucaaca uugaagacug ucggaaguuu     2160

aucgauuucu auaaacaguc cauuucaaag caucccgagu ggaaggacuu cgguuuucgc     2220

uucagugaua cccaacgaua caauucuaua gacgaauuuu acagagaggu cgagaaccag     2280

ggguauaaau ugacauucga aaauaucagc gaguccuaca uugauucagu gguuaaccag     2340

ggaaagcugu aucucuuuca gaucuacaau aaagacuuca gcgcuuauuc uaagggcagg     2400

ccuaaccugc acacuuuaua cuggaaagcc cuguuugaug aacggaaucu ucaagacgug     2460

gucuauaagu ugaacggcga ggcagaacuc uucuaccgua agcaguccau ucccaaaaag     2520

aucacccauc cagccaaaga agcuauagcc aauaagaaca aagauaaucc uaagaaagag     2580

aguguguuug aauacgaccu gaucaaggac aagcgcuuca cagaggauaa auuuuucuuc     2640

cacugcccaa uuacuaucaa cuuuaagagc ucuggggcaa auaaauucaa cgacgaaauu     2700

aaccuacugc ucaaggagaa agcgaaugau guacacaucc uguccauaga cagaggagag     2760

aggcaucuug cuuauuacac cuugguugau gguaagggca acauuaucaa gcaggacaca     2820

uuuaauauca uugggaacga ucggaugaaa accaauuauc acgacaagcu ggccgcaaua     2880

gaaaaagauc gagacucagc ccgcaaggau uggaaaaaga ucaacaauau uaaggagaug     2940

aaagaaggau acuuaaguca ggugguccau gagaucgcua agcucgugau ugaauauaac     3000

gccaucgucg uguucgagga ccugaauuuu ggcuucaaaa gagguagguu uaagguugag     3060

aaacaagugu accagaaacu ugaaaagaug cugauagaga aauugaacua ucucguauuc     3120

aaggauaaug aauuugacaa aacugggggc guccugcggg cauaccagcu aacggcuccc     3180

uucgagacau uuaagaaaau gggaaagcag accggcauca uuuacuaugu gccggccggg     3240

uucacuagca aaaucugucc uguuaccgga uuugugaacc aacuguaccc caaguaugaa     3300

ucggucucca agucucagga guucuucagc aaauuugaca agauuugcua caaucucgau     3360

aaagguuauu ucgaguuuuc auucgacuac aagaacuuug gcgauaaagc ggccaagggg     3420

aaguggacaa ucgcuaguuu cggaucccgc cugauaaauu uuagaaacuc ugacaaaaau     3480

cacaacuggg auaccaggga aguguaucca acuaaggagc uugaaaaauu gcugaaggac     3540

uacagcauug aguacggcca uggugagugu aucaaagcag ccaucugcgg ggaauccgau     3600

aagaaguucu uugcuaaauu aacaucagua cucaacacga uucugcagau gcguaauagc     3660

aagaccggca cugagcuuga cuaucugauc aguccugugg ccgauguuaa cggaaauuuc     3720

uuugacucuc ggcaagcacc caaaaacaug ccacaggaug cggacgccaa uggcgcuuac     3780

cacauagggc uaaagggacu caugcuguug ggucgaauua aaaacaauca ggaaggcaag     3840

aagcugaacu uagucaucaa aaaugaggaa uauuucgagu uugugcagaa cagaaauaac     3900

uga                                                                   3903


<210>  3460
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3460
augaagcccg acaucaaaga uagagaguac auguauaggc ugauuauagg ccaacucuuc       60

uacgacgggc accagcagau cgccgugaac cuugcuaaua cccugggaug cagcgcaccu      120

ccaccgcccc cuuccgauaa guuguuucgg uuagucacaa uugccaaaca guucguugaa      180

gacccagauu auaaggcgga ggaaaacacu augcccaugc aguuugagac gucuucagcu      240

ggucuggacc uacaauacga ugccgacgug gcagccacca guccugaacc aagccaguau      300

gagacaaucu accucacugu acauaaagcu cccugucgcg cagccgcuuu caauuccgau      360

ggcucucuga uugccaccgg gucggcggac ugcucaauca agauaaugga uguggagcga      420

auccuugcac gugaaaagga gcacagagaa augaacgaga auggaccuga cgcccaucac      480

ccagucauua ggacacugua ugaucaugug gacguucggc gcgacuuugg cagauucagc      540

gauaaaaucu uggacuuuca ggaugugaac accgucauuu uccacccgag ggacgcuauc      600

cucauaagug guuccaauga uaagacugug aaacuguuug acuucucuaa gacagccgua      660

aaacgggcaa ugaagacccu gagcgagguu uaccccgugc gcgcucucuc cugucacccu      720

gggggagaau uuuuacuggu cgccacggau cauccaacug ugcgacuuua caacauugag      780

accgcgcagg ccuaugcauc agcuaauccc gacgaucaac acagagaaag ugugacagac      840

guccauuaca gcgagaacgc cagguuguau guuacugcuu cuaaggaugg cgacgugaaa      900

guaugggacg gcguguccuc acggugcguc gaaaccuuca agcgugccca cgaugggagc      960

aguaucuguu cugcaaaauu uacaaagaau ggaaaauaca uucugaccuc cgguauggac     1020

agcaucguga agcuauggga gcucucgacg aaccgcugcc ugaucguuua uacuggcgcc     1080

ggggcuacag gagcgcagga uuacccuacc ucugcauccu ucaaucagaa cgaggacuuc     1140

gugcuuuuuc cagaugaaaa aucaggcagu auguguagcu gggaggccag aaaugcugac     1200

aggaagcggc uguuggcccu cggucacacu uccgcaugcc gaacauucgu ccauucuccc     1260

agcaugccgg ccuuuaugac cgggucagau gaccaccgcg cuagauucug guauaggaag     1320

ccuacgacca acgauaacug a                                               1341


<210>  3461
<211>  1293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3461
augaagcccg acaucaaaga uagagaguac auguauaggc ugauuauagg ccaacucuuc       60

uacgacgggc accagcagau cgccgugacc cuugcuaaca cacugggaug cagcgccccu      120

gcaccaccgc ccuccgauaa guuguaucgg uuagucacua uugcgaaaca guuuguugaa      180

gacccugauu acaaggcuga ggaaaaaucu cugccaaugc aguucgagac ggugucaacc      240

ggucuagacc ucgaauauga ugccgacgua caacccguga guccugagcc aagccaguac      300

gagacaaucu aucugacugu ccauaaggca cccugucgcg ccgcugcauu uaauuccgau      360

ggcucucuug uggccaccgg gucggcugac ugcucaauua agaucaugga uguugaacga      420

auacuggccc gugagaaaga acacagagag augaacgaga auggaccuga cgcgcaucac      480

ccagugauca ggacauugua cgaucauguc gacgacguga acaccguacu cuuccacccg      540

cgggaugcaa uucugaucag cggcaguaau gacaagacug uuaaacuguu ugauuucucc      600

aagacagccg ugaaacgcgc uaugaagacc cucucugaag ucuaccccgu gagagccuua      660

agcugucacc cuggugggga guuucugcuu guggcaacgg accauccaac ugucagguug      720

uauaacauug aaaccgcuca ggccuacgcg uccgccaauc ccgaugacca gcaccgggag      780

ucaguuacag augugcauua uagugaaaac gcacgccugu acguaacugc uagcaaggac      840

ggagauguga aaaucuggga cggcgucucu aaucgaugcg uggagaccuu caagagagcc      900

cacgauggcu ccgcuauaug uucagccaaa uuuacaaaga acgggaaaua ucuacucacc      960

agcggaaugg acaguauugu uaagcugugg gagcuuucua cgaauaggug ccugaucgug     1020

uacacuggug caggcgccac aggggcucaa gacuaugcga ccuccgcaag uuucaaccac     1080

aaugaagauu acgucuuguu uccugacgag aaaucgggau cuauguguuc cugggaugcc     1140

cggaacucag accguaagcg ccuccuggcu uuaggccaua cuagcgccug cagaacauuc     1200

gugcacagcc cauccaugcc cgcauuuaug accgguucug augaccauag ggcccgguuc     1260

ugguaccgaa agccggcuac gaccgauaau uga                                  1293


<210>  3462
<211>  3690
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3462
auggccgcua gcaagcugga gaaauucacc aacugcuacu cccucucuaa gacacuuaga       60

uuuaaagcaa uccccguggg caagacucag gaaaauauug acaacaaaag gcuguugguc      120

gaggaugaaa agcgggccga ggacuauaag gggguuaaaa aguuacugga ucgcuacuau      180

cuaucauuca uaaaugacgu gcuccacagu aucaaacuga agaaccuuaa uaacuacauu      240

agccuguuua ggaaaaagac gcguaccgaa aaggagaaua aagaguugga aaaccucgag      300

aucaaucuga gaaaggaaau ugcgaaggcu uucaagggag ccgcagguua uaaaucccug      360

uuuaagaaag auaucauaga gacaauccuc ccugaggccg cugacgauaa ggacgaaauu      420

gcauuaguaa acucuuucaa uggcuuuacu accgccuuca caggguuuuu cgauaacagg      480

gagaauaugu uuucggaaga ggcuaaguca accagcaucg ccuuccggug uauuaacgaa      540

aaucugacuc gcuacaucag uaacauggac auauuugaga aaguggaugc gauuuucgac      600

aagcaugagg uccaagaaau caaagagaag auucuuaacu ccgauuauga cguggaagac      660

uucuuugagg gagaguucuu uaauuucguu uugacacagg aaggcaucga uguguacaac      720

gcaaucauug guggguuugu caccgagucu ggagaaaaga uaaagggccu gaaugaguac      780

aucaaccuau auaaugccaa gacgaaacag gcucucccaa aguucaaacc gcuguacaaa      840

caggugcuua gcgacagaga aucccuguca uuuuauggcg agggguacac uucugaugaa      900

gagguauugg agguuuucag gaacacccuc aauaaaaaca gcgaaauuuu uaguuccauc      960

aagaagcugg agaaauuauu caagaauuuu gacgaauauu caagcgccgg aauauucgug     1020

aaaaacgguc ccgcaauuag uacaaucucu aaggauaucu uuggcgagug gaaucugauu     1080

cgggacaaau ggaacgcuga guacgaugac auccaccuca agaagaaagc cgucgugacu     1140

gaaaaguaug aggaugaccg ccgaaaaucc uucaagaaaa uugggagcuu cucgcuugaa     1200

caacugcagg aguacgcgga ugccgacuug ucuguggucg aaaagcuaaa agagauaauc     1260

auucagaagg uugacgagau cuacaaggug uauggauccu cagaaaaacu guuugaugca     1320

gacuucguac ucgagaagag ucugaaaaag aaugaugcug uggucgccau caugaaagac     1380

cuucuggaua gcgugaaguc cuuugaaaac uacauuaagg cuuucuuugg cgaggguaaa     1440

gaaaccaaua gagacgaguc uuucuauggg gauuuuguuu uggccuacga cauacuccug     1500

aagguggauc auaucuauga cgcaauuagg aacuacguca cacagaaacc uuauagcaag     1560

gauaaauuca aguuauacuu ucaaaaccca caguucaugg gaggcuggga caaggauaaa     1620

gagaccgacu accgggccac gauccugcgu uauggcucaa aguacuaucu agcuauuaug     1680

gacaaaaagu acgcgaaaug ccuccagaag aucgauaagg acgaugugaa ugggaacuau     1740

gaaaagauaa auuacaagcu gcuucccgga ccuaacaaaa uguugccaaa gguauuuuuc     1800

aguaaaaagu ggauggcaua uuacaauccc uccgaggaca uccagaaaau uuacaagaac     1860

gguacuuuua agaaaggcga uauguucaau cugaacgacu gucacaagcu caucgauuuc     1920

uuuaaagaca gcauuucucg cuauccuaag uggucaaaug ccuacgauuu caacuuuagu     1980

gaaacagaga aauauaagga caucgcuggg uucuacagag aaguugagga gcaaggauau     2040

aaggugagcu uugaauccgc cucuaaaaag gaggucgaua aacuggugga agagggcaaa     2100

cuuuacaugu uccagauaua uaauaaagac uuuucggaua agagccaugg uaccccaaac     2160

cugcacacua uguacuucaa guugcucuuu gacgagaaua accacgggca gauuaggcug     2220

uccggcggag cagaauuauu caugcggcga gccucacuga aaaaggagga gcuagucgug     2280

cauccggcua auagucccau cgccaacaaa aacccugaca auccaaagaa aacaaccacg     2340

cucucuuacg auguuuauaa ggacaagcgc uuuagcgaag aucaauacga acugcacauu     2400

cccaucgcaa uaaacaaaug cccuaagaau aucuucaaaa uuaacaccga ggugagagua     2460

cuuuugaagc augacgauaa uccauauguc aucggcauug accgagggga gcggaaccug     2520

cucuacaucg ugguugugga uggaaaaggu aauauagucg aacaguauuc ccugaacgag     2580

auuaucaaua acuucaaugg cauccgcauu aagacagacu accacucauu acuggauaag     2640

aaagaaaagg agagauuuga agcgaggcag aacuggacua gcaucgagaa uauuaaagag     2700

cuuaaggcug gguauauauc ucagguggua cacaaaaucu gugaauuggu ggagaaguac     2760

gacgccguua uugcacucga agaucugaac uccggauuca aaaauagucg ugucaaggug     2820

gagaagcaag uguaccagaa auuugaaaag augcuaaucg acaaacugaa cuauaugguc     2880

gacaagaaaa gcaaccccug cgccaccggc ggugcucuca agggguacca gauuacaaau     2940

aaguucgagu cuuuuaaauc caugucaacu cagaacggcu ucaucuuuua uauaccggcc     3000

uggcugacca guaagaucga uccuagcaca ggauucguua aucuuuugaa aaccaaguac     3060

acuucgauug cagacuccaa aaaguuuauc ucuagcuucg aucggauuau guaugugccc     3120

gaggaagacc uguuugaguu cgcuuuagau uacaagaacu uuucacgaac ggacgccgau     3180

uauaucaaaa aguggaaacu cuacaguuac ggcaauagaa uaaggauuuu cgcggccgcu     3240

aagaaaaaca auguguucgc augggaagag guaugucuga cauccgccua uaaggagcuu     3300

uuuaacaagu acgggaucaa uuaucaacag ggagacaucc gcgcucuguu gugcgaacag     3360

ucugauaaag ccuucuacag cuccuuuaug gcacucaugu cacugaugcu acagaugcgg     3420

aacagcauua ccgguagaac ugacgucgau uuccugauaa guccagugaa aaauucugac     3480

ggcaucuuuu augauuccag gaacuacgag gcgcaagaaa augccauucu cccuaaaaac     3540

gcugacgcca auggggcaua uaacaucgcu cgcaagguuc ugugggccau uggacaguuc     3600

aaaaaggcag aggacgaaaa acuugauaag gugaagaucg ccauaagcaa uaaagagugg     3660

uuggaguacg cucagaccuc ggucaaguga                                      3690


<210>  3463
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3463
augagcaucu accaggaguu cgugaacaag uauucccugu cuaaaacccu cagauuugaa       60

cuuauucccc aaggcaagac acuggagaau auaaaagcca ggggguugau cuuagacgau      120

gaaaagcggg cuaaagacua caagaaggca aaacagauua ucgauaagua ucaccaguuc      180

uuuauugagg aaauccuguc aagugucugc auaagcgagg accuacucca gaacuacucc      240

gauguuuauu ucaaacugaa gaaaucugac gaugacaauc uucaaaagga uuuuaagucg      300

gccaaagaca cuaucaagaa acagauuuca gaguacauca aggauagcga aaaauucaag      360

aaccuguuua aucagaacuu gauugacgcg aaaaagggac aggagaguga ucucauccug      420

uggcugaagc aauccaaaga caaugguaua gaacucuuca aggcuaacuc ugacauuacg      480

gauaucgacg aggccuuaga gauuaucaaa aguuuuaagg gcuggaccac auauuucaaa      540

ggguuucaug aaaaucgcaa gaacguguac uccucagaug acaucccuac uagcauuaua      600

uaccgaaucg uagaugacaa ucugccaaag uuccuugaga acaaagcaaa guaugaaagu      660

uugaaagaua aggccccgga ggcuauuaau uacgaacaga ucaaaaagga ccuggcagag      720

gagcuaaccu uugauauaga cuauaagaca ucugaaguga accagcgugu cuucucccuc      780

gaugaggugu ucgaaauugc caauuuuaac aacuaccuga aucagucagg aaucaccaaa      840

uucaacacua ucauuggcgg uaaguuuguu aauggggaga acacaaaaag aaagggaauc      900

aaugaauaua uuaaccuuua cagccaacag auaaaugaca aaacccugaa gaaguauaaa      960

augagugugu uguucaagca gauccucucu gacacggagu ccaaaucauu ugucauugau     1020

aagcuggagg acgauucgga cgugguaacu accaugcaau cuuucuacga acagaucgcu     1080

gccuuuaaaa caguugagga aaaguccauc aaagagacuu uaucacuccu guucgaugac     1140

cuuaaggcgc agaagcugga uuugaguaaa auuuacuuua agaacgacaa aagccuaacc     1200

gaucuguccc agcaaguguu cgacgauuau ucugucauag gcacagcagu gcucgaauac     1260

aucacccaac agguggcccc caagaaucug gacaacccua gcaaaaagga gcaggaucuu     1320

auugcuaaga aaacggagaa ggccaaauau cugucauugg aaacuaucaa gcucgcacug     1380

gaggaauuua auaaacacag ggacauugac aagcaguguc gguucgagga gaucuuagcu     1440

aacuuugccg cgauaccaau gaucuucgau gaaauugccc agaauaagga caaccuggca     1500

cagaucaguc uaaaauacca gaaucaaggc aagaaagauc uccugcaggc uuccgccgag     1560

gaagacguca aggcuauuaa agaucuuuug gaccagacaa acaaccugcu ccaucgccug     1620

aagaucuucc acauaagcca gucugaggau aaggccaaua uucuugacaa agaugaacau     1680

uuuuaucugg uuuucgagga gugcuacuuu gaauuggcaa acaucgugcc ccucuauaau     1740

aagauuagaa acuacaucac ccaaaaaccu uacucagacg agaaguucaa acugaauuuu     1800

gaaaacagua cuuuagccaa ugggugggau aagaacaaag agccagacaa uacagcuaua     1860

cuguucauca aggaugacaa guauuaccua ggaguaauga acaaaaagaa uaacaaaauu     1920

uuugacgaua aggcgaucaa agaaaauaag ggugagggcu auaagaaaau uguguacaag     1980

cuccugcccg gggcaaacaa aaugcuuccu aaggucuucu uuagcgccaa auccaucaag     2040

uucuauaauc caucugagga cauauugagg auucggaacc acucgaccca cacgaagaac     2100

ggaaauccgc agaaaggcua cgaaaaguuu gaguucaaca ucgaagauug ucgcaaauuu     2160

aucgacuucu auaaggagag cauuuccaaa caucccgaau ggaaggauuu cgguuuucga     2220

uucucagaca cccagagaua caauaguauc gaugaguuuu acagggaggu ggaaaaccaa     2280

ggguauaagc ugacauucga gaauauuucu gaaagcuaca uagacuccgu ugugaaccag     2340

ggaaaacucu aucuguuuca gaucuacaau aaggauuucu cagcuuauag caaaggccgg     2400

ccuaacuuac acacucugua cuggaaggcc cuuuuugacg agcguaauuu gcaggauguc     2460

guguauaaac ucaacggcga ggcagaacug uucuaccgca agcaaucuau uccaaagaaa     2520

aucacccauc ccgccaagga ggcuauugcc aauaaaaaca aggacaaucc uaaaaaggaa     2580

uccguauuug aguacgaccu aaucaaagau aagagauuca cagaagacaa guuuuucuuc     2640

cacugcccaa uaacuaucaa cuuuaaaagu agcggggcaa auaaguucaa cgaugagauu     2700

aaccugcucc ugaaagagaa ggcgaaugac guucacaucc uuucuauuga uaggggagaa     2760

cggcauuugg cuuauuacac ccugguggac gguaaaggca acaucauaaa gcaggauaca     2820

uuuaauauua ucgggaacga ccgaaugaag accaauuauc acgauaaauu agccgcaauc     2880

gagaaggacc gcgauuccgc cagaaaagac uggaagaaaa uuaacaauau aaaggaaaug     2940

aaggagggau accucucaca ggucgugcau gaaaucgcua aacuggucau ugagcacaac     3000

gccaucgugg uuuucgagga ucuuaauuuu ggcuucaaga ggggucgguu uaaaguggaa     3060

aagcagguau aucaaaaacu ggagaagaug uugauugaaa agcucaacua ccuggucuuc     3120

aaagacaaug aguuugacaa gacugggggc gugcuacgcg cauaucagcu gacggcuccc     3180

uucgaaacau uuaaaaagau gggaaaacag accggcauca uauacuacgu uccggccggg     3240

uucacuagca agaucugucc ugugaccgga uuugucaacc agcucuaucc caaauacgag     3300

agcgugucga agucccaaga guucuucucu aaguuugaua aaauuugcua uaaucuggac     3360

aaggguuacu ucgaauuuag cuucgauuau aaaaacuuug gcgacaaggc ggccaaaggg     3420

aaguggacaa ucgcuucauu cggaaguaga cuuauuaauu uuaggaacuc cgauaagaau     3480

cauaacuggg acacccguga gguauaccca acuaaagaau uggagaagcu guuaaaagau     3540

uauucuaucg aguacggcca cggugaaugu auaaaggcag ccauuugcgg ggagagcgac     3600

aaaaaguucu uugcuaagcu cacauccgug cugaacacga uccuucagau gcggaauagc     3660

aaaaccggca cugaacugga uuaccuaauc agcccuguug ccgacgucaa cggaaauuuc     3720

uuugauaguc gacaggcacc caagaacaug ccacaagacg cggaugccaa uggcgcuuau     3780

cacauugggc ucaaaggacu gauguugcug gacagaauca agaacaauca ggaggguaaa     3840

aaguuaaacc ucgugauaaa gaaugaagag uacuucgagu uugugcagaa caggaauaac     3900

uga                                                                   3903


<210>  3464
<211>  1071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3464
augaagacca acuaccacga caaacuggcc gcuaucgaga aggauagaga aagcgcaagg       60

aaagacugga agaaaauuaa uaacauaaag gagaugaagg aaggcuaucu cucccaggug      120

guccaugaga ucgccaaacu uguuauuggg uacaaugcga ucgugguauu cgaagaucug      180

aacuuuggau ucaagcgggg ucgcuuuaaa guggagaagc aagucuauca gaaauuggag      240

aagauguuaa uugaaaagcu gaauuaccua guguucaaag acaacgaguu ugauaaggcu      300

ggcgggguuc uccgagccua ucagcugaca gcacccuucg aaacuuuuaa aaagauggga      360

aaacagacgg gcaucauaua cuaugugccu gccgacuuca ccucuaagau cugcccaguc      420

acagguuuug ugaaucaacu uuacccgaaa uacgagucag uaaguaagag ccaggaguuc      480

uuuuccaagu ucgauaaaau uuguuauaac cuggacaagg gguacuuuga guucucuuuc      540

gauuauaaaa auuuuggaga caaggcugca aaaggcaagu ggacuaucgc cucguucggc      600

ucacguuuga uuaacuuuag aaauagcgau aagaaccaca auugggacac cagggaaguu      660

uaccccacaa aagaacucga gaagcugcug aaagauuaua guaucgaaua cgggcaugga      720

gagugcauaa aggcugccau uuaugcggag aacgacaaaa aguucuuugc aaagcucacc      780

uccaucuuaa auucuauucu gcagaugcgg aacagcaaaa cugguacaga acuugacuac      840

uugaucuccc cuguggccga ugucaacggc aauuucuuug acucacgcca cgcuccaaag      900

aacaugcccc aggaugccga cgcaaauggg gcuuaccaua ucggacugaa aggccuaaug      960

cuccuguaua gaauuaagaa caaucaagau gguaaaaagc ugaaccuugu gauaaagaau     1020

gaggaauacu ucgaguuugu gcagaacagg aauaaaagua gcaagaucug a              1071


<210>  3465
<211>  3792
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3465
auggaggacu acagcggcuu cgugaacauc uauuccauuc agaagacccu gagauuugaa       60

cucaaacccg ucgggaagac acuugagcac auagagaaaa agggauuccu gaaaaaggau      120

aagaucaggg ccgaggacua caaagcuguu aagaaaauua ucgauaagua ucaucgggca      180

uacauugaag agguguuuga cucuguauug caccagaaaa agaagaaaga uaagacucgc      240

uucucaacgc aauuuaucaa agagauaaag gaguucagug aguuauauua caaaaccgaa      300

aagaauaucc cugacaaaga gcgacuggag gcccuaagcg aaaagcuccg uaagaugcug      360

gugggugcgu uuaaaggcga guucuccgaa gaggucgcug aaaaguauaa aaaccuguuu      420

ucuaaggagc uuauuagaaa ugagaucgaa aaauucugcg agacagauga agagaggaaa      480

caggugucga acuuuaaguc auucacuacc uacuuuacag gguuccauag caaucggcag      540

aacauuuaca gugacgagaa aaaguccacc gccaucggau aucgcauaau ucaccaaaau      600

uugccaaaau uccucgauaa ccugaagauc auugaaucua uccagagaag guuuaaagac      660

uucccgugga gcgaucugaa gaagaaucuc aaaaagaucg acaaaaacau uaaguuaacu      720

gaguacuuuu ccauagaugg cuucguuaau gugcugaacc agaaagguau cgacgcauau      780

aauacaauuc uugggggaaa gucagaagag aguggcgaaa agauccaggg cuugaacgag      840

uacauaaauc uguaucggca gaaaaacaac auugaccgca agaaucuacc caacgucaaa      900

auccucuuua agcaaauccu gggggaucga gaaaccaaga gcuucauucc ugaggccuuu      960

ccagacgauc agucugugcu uaauuccauc acggaguucg cuaaguaccu gaaauuggac     1020

aagaagaaaa agucaauuau agcagaacuc aaaaaguuuc ugagcaguuu caacagauau     1080

gaguuagaug gaaucuaccu ggccaaugac aacucucucg cuuccauuag cacuuuucuu     1140

uucgaugacu ggucguuuau caaaaagucu guauccuuca aguacgauga aucaguuggu     1200

gaccccaaaa agaaaaucaa gaguccucug aaauaugaga aggagaagga aaaaugguug     1260

aagcagaaau acuauaccau uagcuuccua aaugaugcca uagaguccua cucuaagagc     1320

caagacgaga aaagggugaa gauccggcug gaagcguauu uugcagaguu caagucaaaa     1380

gacgaugcca agaaacaguu ugaccuccug gaacguauug aggaggcuua cgccaucguc     1440

gaaccacuuc ugggcgcaga auauccccgc gauagaaacu ugaaggcuga caaaaaggag     1500

guggggaaaa uuaaggauuu ccucgacagu aucaaguccc ugcaguuuuu cuuaaaaccu     1560

cugcuaagcg ccgaaauauu ugaugagaag gaccucggau ucuacaauca acuggagggc     1620

uacuaugaag agaucgauuc uauuggucau cuuuacaaca aagugaggaa uuauuugaca     1680

gggaagaucu acucaaaaga aaaguuuaag cugaacuucg agaauaguac ucuccugaaa     1740

ggaugggacg aaaaccggga ggucgcgaau cuuuguguua uuuuucgaga ggaucagaag     1800

uauuaccugg gcgugaugga caaagaaaac aacaccaucu ugagcgauau accaaaggua     1860

aaaccgaaug aacucuucua ugaaaagaug guguacaagc ugauucccac accucacaug     1920

caguuaccac gcaucaucuu uuccucugac aaccugucga uuuacaaucc cagcaaaucc     1980

auacuaaaga ucagagaggc caaaucauuc aaggagggca aaaacuucaa gcucaaggac     2040

ugccacaaau uuauugauuu cuauaaggaa aguaucucua aaaaugagga cuggagcagg     2100

uuugauuuca aguuuuccaa aaccucaagc uacgaaaaca uuucugaguu cuaucgggaa     2160

gucgagcgcc agggguacaa ucuggacuuu aagaaggugu ccaaauucua uaucgauagu     2220

cuuguugagg acggaaaguu guaccuguuu caaauauaua acaaagauuu cagcaucuuu     2280

ucuaagggua aaccuaaucu ccauacgauu uacuucagau cccuguucuc aaaggaaaac     2340

uuaaaagacg ugugucugaa acuuaauggc gaggcagaaa uguuuuucag gaagaaaagu     2400

aucaacuacg augagaagaa aaagcgugaa gggcaccauc cagaguuguu ugagaaacuc     2460

aaguauccca uucugaagga caaacgguac agcgaagaua aguuccaguu ucaccuaccg     2520

aucucgcuga auuucaaauc caaggagcga cucaacuuua aucugaaagu caacgaguuc     2580

cuuaagagaa acaaggacau uaauauaauc ggaauugaua ggggcgaacg caacuugcug     2640

uauuuaguga ugaucaauca gaaaggugaa auucucaagc agacucugcu ugacucuaug     2700

caaagcggga aaggccggcc ugagaucaac uacaaggaga aacugcagga aaaggagaua     2760

gaaagagaca aggcuaggaa aucaugggga acaguagaga auaucaagga guugaaagaa     2820

ggcuaucuca guauuguuau ccaccagauu uccaagcuga ugguggagaa caaugccauc     2880

gucgugcuag aagaucugaa cauaggguuu aaacgcggac gacagaaggu cgagcggcaa     2940

guguaccaga aauucgaaaa gaugcucauu gacaagcuga auuuucuugu uuucaaagag     3000

aacaagccca ccgagccagg uggcguguug aaggcuuauc agcugacuga ugaauuucag     3060

ucuuucgaga aguuaagcaa acaaacaggg uuccucuuuu acguaccuuc cuggaauacc     3120

ucaaagaucg acccccguac gggauucauc gauuuucugc auccagccua cgaaaacauu     3180

gagaaggcaa aacaguggau aaauaaguuc gacagcauca gauuuaacag uaaaauggau     3240

ugguucgaau uuaccgccga cacaaggaag uucucugaga aucuuaugcu gggcaaaaac     3300

cgcgucuggg ugauuugcac uaccaacguu gagcgguauu uuacauccaa gacugcuaau     3360

agcucgaucc aguacaacuc aauucaaauc accgaaaagc uaaaagaacu cuucguggau     3420

auacccuuuu cuaaugguca ggaccugaag ccugaaaucu ugaggaaaaa cgaugcgguc     3480

uucuuuaaga gucuguuauu cuauauuaaa acaacccucu cccugagaca gaauaacggg     3540

aagaagggcg aggaagagaa agacuucauc cuuagcccag ugguagacuc uaagggacga     3600

uuuuucaaua gccuggaggc auccgaugac gaaccgaaag augccgacgc uaacggcgcc     3660

uaccacauug cauugaaggg gcucaugaau cugcuagugc ugaacgagac uaaagaagag     3720

aaucuuucac gccccaagug gaaaaucaag aacaaggauu ggcucgaguu uguuugggaa     3780

cggaauagau ga                                                         3792


<210>  3466
<211>  2820
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3466
augaaccugu acagcaaucu caccaacaag uauucccuuu cuaaaacacu gagauucgag       60

uugauccccc agggcgaaac uuuagagaau auuaaggcca gggggcugau acuagacgau      120

gaaaaacggg cuaaggacua caaaaaggca aagcaaauca uugauaaaua ucaccaguuu      180

uucaucgagg aaauucucuc aagugugugc aucagcgagg accugcuuca gaacuacucc      240

gaugucuauu uuaagcugaa aaagucugac gaugacaauu ugcagaaaga uuucaagucg      300

gccaaggaca cgaucaaaaa gcauauauca cgcuacauua acgauagcga gaaauuuaag      360

aaucucuuca accaaaaucu gaucgacgcg aaaaagggac aggaaaguga ucugauucuc      420

ugguuaaaac aguccaagga caacgguauc gagcuguuua aggcuaauuc ugacauaacc      480

gauauugacg aagcccuuga gaucauuaag agcuucaagg gcuggacaac uuauuuuaaa      540

ggguuccacg agaaccgaaa gaauguuuac uccucagaug acaucccuac caguaucauu      600

uaccguauag uggaugacaa cuugccaaaa uuuaucgaaa auaaggcaaa guaugagaac      660

cugaaagaua aggccccgga agcuauuaac uacgagcaga ucaaaaagga ccuagcagag      720

gaacucacau ucgauauaga cuauaaaacc agcgagguaa aucaaagagu guuuucucug      780

gaugaagucu ucgagauugc caacuucaau aacuaccuua aucaguccgg aaucacuaag      840

uuuaacacaa ucauuggcgg uaaguucgug aauggggaaa acaccaaaag gaagggaauc      900

aaugaguaua uuaaccugua cucacagcag auaaaugaca aaacguugaa gaaauauaag      960

augagcguuc ucuuuaagca aauccugagu gacacugaau cuaaauccuu cgugauugau     1020

aaguuagagg acgauagcga cgucgugacc acaaugcagu cguuuuacga gcagaucgcu     1080

gccuucaaaa cucuggaaga gaagucuauc aaagaaaccc ucucccuucu guuugaugac     1140

uugaaggcgc aaaaacuaga ucugucaaag auuuacuuca agaacgacaa aagucucaca     1200

gaucugagcc agcagguauu ugacgauuau uccguuauag gcaccgcagu gcuugaguac     1260

aucacgcagc aagucgcccc caagaaucug gacaacccuu cuaaaaagga acaggauuug     1320

auugcuaaaa agacugagaa ggccaaauau cucagccugg agacaaucaa guuagcacug     1380

gaagaguuca auaaauaccg ggacauugac aagcaguguc gcuuugaaga gaucuucgcu     1440

ucauuugccg auauaccagu gcuauucgac gagaucgcgc agaacaaaaa caaucucgcc     1500

caaauuagua ucaaguauca gaaccagggc aagaaagauc ugcuucagac cuccgcagaa     1560

guggacguca aggcuauuaa agauuugcug gaccaaacua auaaccuccu gcauaaacuu     1620

aaaaucuucc acauaacaca gagcgaggau aaggccaaua uucuggacaa ggaugaacau     1680

uuuuacuugg uuuucgacga gugcuauuuu gaacucgcua acaucguggc ccuguacaau     1740

aaaauuagaa acuacaucac ccagaagccc uauucugaug agaaauucaa guuaaauuuu     1800

gagaacucaa cgcuggcaaa ugggugggac aaaaacaagg aaccugauaa uaccgccaua     1860

cuauucauca aagacgacaa guacuaucuc ggaguaauga acaagaaaaa uaacaagauu     1920

uuugaugaca aagcuaucaa ggagaacaaa ggugaaggcu acaagaaggu ggucuauaaa     1980

cugcuuccag gggcgaauaa gauguugccc aaaguguucu uuagugcaaa gagcauuaac     2040

uucuacaauc cuuccgagga uauccugagg auacggaacc acucuacaca cacuaaaaau     2100

ggaucgccac agaagggcua ugaaaagcuc gaguuuaaca uugaggacug ucgcaaauuc     2160

aucgauuuuu acaagcauag caucucccga cacccggaau ggaaagacuu cgguuucaga     2220

uuuucagaua ccaagaaaua caauaguauu gacgaguucu auagggaagu ugagaaccaa     2280

ggguacaagc ugacauuuga aaauaucucu gagagcuaua uugauuccuu aguggacgag     2340

ggaaagcugu accuuuucca gauauauaac aaagauuuuu cagucuacag caagggcaaa     2400

cccaauuugc auacucucua uuggaaggcc cuguucgacg aacggaaccu acaggacgug     2460

guauacaaac ugaauggcga ggcugaacuc uuuuaccgua agcaaucuau cccuaagaaa     2520

auuacccacc cagccaagga ggcaaucgcc aacaaaaaua aggauaaccc caaaaaggag     2580

uccauuuucg aauaugaccu gaucaaagau aagcgcuuua cagaggacaa guucuuuuuc     2640

cacugcccua uaaccaucaa cuucaaaagu agcggggcua auaaguuuaa cgaugaaauu     2700

aaucuuuugc ugaaagagaa ggccaacgac guucauaucu uaucuauuga uagaggagaa     2760

aggcaccucg cauacuauac ucugguggac gguaaaggca auaucauaug uaagaacuga     2820


<210>  3467
<211>  3795
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3467
augcuguucc aggaguuuac ccaccucuac ccccuuagca agacagugag auucgaacug       60

aaaccuaucg gcaagacuuu ggagcauauu cacgccaaag acuuuuuauc ccaagaugaa      120

acgauggcug acauguauca gaaggucaaa gcaauacugg augacuacca uagggauuuc      180

aucgccgaca ugaugucugg gguugugcua accaagcucg cggaguuuua ugaaguauac      240

cuggcccuuc ggaagacacc aaaagaugac ucacugcaga agcaguugac ugagauucaa      300

accgcucucc gcaaagagau cgugaagccg auuggaagug guggcaaaua uaaggcaggg      360

uacgaucgac uguucggagc caagcuguuu aaagacggca aggaacucgg ugauuuagcu      420

aaauucguca ucacacagga gggggaaagc ucccccaagc uggcacacau agcccauuuu      480

gagaaauucu cuaccuauuu uacuggauuc cacgacaacc guaagaauau guacucggau      540

gaggacaaac acacagcuau cgccuacaga cuuauucaug aaaacuugcc uagguuuauc      600

gacaaucugc agauucuagc gaccaucaag cagaagcacu cagcacucua ugaucaaaua      660

gugaacgagc ugaaugccaa cggccuugac guuagccugg cuagucauuu ggauggcuac      720

cacaaacucc ugacgcagga agggauuacu gccuauaaua ccuuacuggg agguaucucc      780

ggcgaggcag ggucucggaa gauucaggga aucaacgaac ucaucaauau ucaccauaac      840

cagcacugcc aaaagagcga gcgcauagcu aaacuuagac cacugcauaa acagaucuug      900

uccgacggca uggguguguc auuccuaccc aguaaguucg ccgaugacag cgaggucugu      960

caggcgguga augaauuuua caggcacuau gcccauguau ucgcaaaggu ucaaucucug     1020

uuugaucggu ucgacgauua ccgcaaagac gggauuuaug uggagcacaa gaaccucaau     1080

gaacugucca aacaggcuuu uggagauuuc gcccuucugg gccgagucuu ggacggcuac     1140

uacguggacg uggucaaccc ugaguuuaau gaaagauucg cuaaggccaa aacagauaac     1200

gcaaaggaga agcucacuaa agagaaggac aaauuuauca aggggguuca cucacuggcc     1260

agcuuagaac aggcuauaga gcauuauauu gcgggacacg augacgaaag ugugcaggca     1320

gguaaacugg gccaauacuu caagcauggg cuagccggag uagauaaccc aauccagaag     1380

auccacaauu cucacuccac cauuaaaggc uuucucgaga gggaacggcc cgcuggugag     1440

cgugcccugc cuaagaucaa aagcgacaag ucgccagaga ugacacagcu ucgccaguug     1500

aaagaacugc ucgauaacgc acugaaugug guccauuucg ccaaacuucu gaccacgaag     1560

acuacauugg acaaccaaga ugggaauuuu uauggcgagu ucggagcucu cuacgacgaa     1620

cuggccaaga uugcaaccuu auauaacaag gugagagauu accugucuca gaaaccguuc     1680

uccacugaga aguauaaacu aaauuuuggc aaccccacac uccugaaugg gugggaccuu     1740

aacaaggaga aagauaauuu cggaguuaua uugcagaagg acgguugcua cuaccuggcg     1800

cuccuggaca aggcucacaa aaagguguuu gauaacgccc cuaaugcagg caaaucaguc     1860

uaucagaaga ugguguacaa auuacugcca gggcccaaca agaugcuucc uaagguauuc     1920

uuugccaaaa guaauuugga cuauuacaac ccaagcgcug aacuccugga uaaguaugcc     1980

caaggaaccc auaaaaaggg caacaauuuc aaccuaaaag acugucacgc acugaucgau     2040

uucuucaagg cuuccauuaa uaagcauccc gaguggcagc acuuugguuu cgaauuuucu     2100

ccgacgagcu cauaccagga ccucagugau uucuauaggg agguugaacc ucagggguac     2160

caagugaaau uugucgacau caacgccgag uacaucgaug agcuggugga acagggccag     2220

cuuuauuugu uccaaauuua caauaaggac uucuccccca aagcgcacgg aaagccaaac     2280

cugcauaccu uauauuuuaa agcccucuuc agcgagaaua accuggcuaa uccuauauac     2340

aaacuuaacg gcgaagcaga gaucuuuuau cggaaggccu cucuggauau gaaugaaaca     2400

acuauucacc gagcugggga ggucuuggag aacaagaauc ccgacaaccc aaagaaacgc     2460

caguucgugu acgauaucau uaaggacaaa agauauaccc aggacaaguu uaugcuccau     2520

guucccauca caaugaauuu cggagugcag gguaugacua uaaaagaauu uaacaagaag     2580

guaaaccaau caauccagca guacaaugau gucaacguga uuggcaucga caggggagag     2640

cggcaucugc uauaccugac cguuauuaau aguaaagggg aaauccucga gcagcgcagc     2700

cugaacgaua uaauuacaac cuccgccaau ggcacucaaa ugacgacacc uuaucacaag     2760

auccuugaca aaagagaaau ugagagguug aacgcacgug uggguugggg cgagaucgaa     2820

accauaaagg agcugaaauc uggguacuua ucgcacgucg ugcaccagau cagccagcuc     2880

augcugaagu auaaugcgau uguagugcuu gaagaucuga acuucggauu uaagcggggc     2940

cgauucaaag uugagaagca gaucuaccaa aauuuugaga acgcccuaau uaaaaagcuc     3000

aaucaucugg cuuugaaaga caaggccgau gacgaaaucg gguccuauaa gaacgcacug     3060

caguuaacua auaacuucac cgaucucaaa ucaauaggaa agcagacagg cuuccuguuu     3120

uacgucccag cuuggaauac caguaaaauu gacccggcca cugguuucgu ggaucuucug     3180

aagcccagau augagaacau cgcacaaucu caggccuuuu ucgggaaauu ugacaagauc     3240

uucuacaacg cugacaaggg auacuuugaa uuuuaugugg auuacgcgaa auuuacagac     3300

aaggccaaaa auagcaggca gacguggguc auuugcuccc acggcgauaa gcgcuauguu     3360

uacgacaaaa ccgcaaacca gaauaagggu gcuacuaucg gcauuaacgu gaaugaugag     3420

uugaaaucac ucuucgcccg guaucauaua aacgacaagc aaccuaaucu ggugauggau     3480

aucugucaga acaaugacaa ggaauuucac aaaagccuaa uguaccugcu uaaggcccuc     3540

cuggcuuuga gauauucuaa cgcauccagu gaugaggacu ucauucugag cccaguagcc     3600

gaugacaaag gggucuuuuu caauucugcg uuagcugaug acacacagcc ccagaacgcc     3660

gacgcaaaug gagccuacca caucgcucuc aagggccugu ggcuucugaa cgaguugaaa     3720

aauuccaacg aucucgacaa gauuaagcug gcaaucgaua aucaaaccug gcuaaacuuc     3780

gcccagaaca gguga                                                      3795


<210>  3468
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3468
augagcaucu accaggaguu cgugaacaag uauucccugu cuaaaacccu cagauuugaa       60

cuuauucccc aaggcaagac acuggagaau auaaaagcca ggggguugau cuuagacgau      120

gaaaagcggg cuaaagacua caagaaggca aaacagauua ucgauaagua ucaccaguuc      180

uuuauugagg aaauccuguc aagugucugc auaagcgagg accuacucca gaacuacucc      240

gauguuuauu ucaaacugaa gaaaucugac gaugacaauc uucaaaagga uuuuaagucg      300

gccaaagaca cuaucaagaa acagauuuca gaguacauca aggauagcga aaaauucaag      360

aaccuguuua aucagaacuu gauugacgcg aaaaagggac aggagaguga ucucauccug      420

uggcugaagc aauccaaaga caaugguaua gaacucuuca aggcuaacuc ugacauuacg      480

gauaucgacg aggccuuaga gauuaucaaa aguuuuaagg gcuggaccac auauuucaaa      540

ggguuucaug aaaaucgcaa gaacguguac uccucagaug acaucccuac uagcauuaua      600

uaccgaaucg uagaugacaa ucugccaaag uuccuugaga acaaagcaaa guaugaaagu      660

uugaaagaua aggccccgga ggcuauuaau uacgaacaga ucaaaaagga ccuggcagag      720

gagcuaaccu uugauauaga cuauaagaca ucugaaguga accagcgugu cuucucccuc      780

gaugaggugu ucgaaauugc caauuuuaac aacuaccuga aucagucagg aaucaccaaa      840

uucaacacua ucauuggcgg uaaguuuguu aauggggaga acacaaaaag aaagggaauc      900

aaugaauaua uuaaccuuua cagccaacag auaaaugaca aaacccugaa gaaguauaaa      960

augagugugu uguucaagca gauccucucu gacacggagu ccaaaucauu ugucauugau     1020

aagcuggagg acgauucgga cgugguaacu accaugcaau cuuucuacga acagaucgcu     1080

gccuuuaaaa caguugagga aaaguccauc aaagagacuu uaucacuccu guucgaugac     1140

cuuaaggcgc agaagcugga uuugaguaaa auuuacuuua agaacgacaa aagccuaacc     1200

gaucuguccc agcaaguguu cgacgauuau ucugucauag gcacagcagu gcucgaauac     1260

aucacccaac agguggcccc caagaaucug gacaacccua gcaaaaagga gcaggaucuu     1320

auugcuaaga aaacggagaa ggccaaauau cugucauugg aaacuaucaa gcucgcacug     1380

gaggaauuua auaaacacag ggacauugac aagcaguguc gguucgagga gaucuuagcu     1440

aacuuugccg cgauaccaau gaucuucgau gaaauugccc agaauaagga caaccuggca     1500

cagaucaguc uaaaauacca gaaucaaggc aagaaagauc uccugcaggc uuccgccgag     1560

gaagacguca aggcuauuaa agaucuuuug gaccagacaa acaaccugcu ccaucgccug     1620

aagaucuucc acauaagcca gucugaggau aaggccaaua uucuugacaa agaugaacau     1680

uuuuaucugg uuuucgagga gugcuacuuu gaauuggcaa acaucgugcc ccucuauaau     1740

aagauuagaa acuacaucac ccaaaaaccu uacucagacg agaaguucaa acugaauuuu     1800

gaaaacagua cuuuagccaa ugggugggau aagaacaaag agccagacaa uacagcuaua     1860

cuguucauca aggaugacaa guauuaccua ggaguaauga acaaaaagaa uaacaaaauu     1920

uuugacgaua aggcgaucaa agaaaauaag ggugagggcu auaagaaaau uguguacaag     1980

cuccugcccg gggcaaacaa aaugcuuccu aaggucuucu uuagcgccaa auccaucaag     2040

uucuauaauc caucugagga cauauugagg auucggaacc acucgaccca cacgaagaac     2100

ggaaauccgc agaaaggcua cgaaaaguuu gaguucaaca ucgaagauug ucgcaaauuu     2160

aucgacuucu auaaggagag cauuuccaaa caucccgaau ggaaggauuu cgguuuucga     2220

uucucagaca cccagagaua caauaguauc gaugaguuuu acagggaggu ggaaaaccaa     2280

ggguauaagc ugacauucga gaauauuucu gaaagcuaca uagacuccgu ugugaaccag     2340

ggaaaacucu aucuguuuca gaucuacaau aaggauuucu cagcuuauag caaaggccgg     2400

ccuaacuuac acacucugua cuggaaggcc cuuuuugacg agcguaauuu gcaggauguc     2460

guguauaaac ucaacggcga ggcagaacug uucuaccgca agcaaucuau uccaaagaaa     2520

aucacccauc ccgccaagga ggcuauugcc aauaaaaaca aggacaaucc uaaaaaggaa     2580

uccguauuug aguacgaccu aaucaaagau aagagauuca cagaagacaa guuuuucuuc     2640

cacugcccaa uaacuaucaa cuuuaaaagu agcggggcaa auaaguucaa cgaugagauu     2700

aaccugcucc ugaaagagaa ggcgaaugac guucacaucc uuucuauuga uaggggagaa     2760

cggcauuugg cuuauuacac ccugguggac gguaaaggca acaucauaaa gcaggauaca     2820

uuuaauauua ucgggaacga ccgaaugaag accaauuauc acgauaaauu agccgcaauc     2880

gagaaggacc gcgauuccgc cagaaaagac uggaagaaaa uuaacaauau aaaggaaaug     2940

aaggagggau accucucaca ggucgugcau gaaaucgcua aacuggucau ugagcacaac     3000

gccaucgugg uuuucgagga ucuuaauuuu ggcuucaaga ggggucgguu uaaaguggaa     3060

aagcagguau aucaaaaacu ggagaagaug uugauugaaa agcucaacua ccuggucuuc     3120

aaagacaaug aguuugacaa gacugggggc gugcuacgcg cauaucagcu gacggcuccc     3180

uucgaaacau uuaaaaagau gggaaaacag accggcauca uauacuacgu uccggccggg     3240

uucacuagca agaucugucc ugugaccgga uuugucaacc agcucuaucc caaauacgag     3300

agcgugucga agucccaaga guucuucucu aaguuugaua aaauuugcua uaaucuggac     3360

aaggguuacu ucgaauuuag cuucgauuau aaaaacuuug gcgacaaggc ggccaaaggg     3420

aaguggacaa ucgcuucauu cggaaguaga cuuauuaauu uuaggaacuc cgauaagaau     3480

cauaacuggg acacccguga gguauaccca acuaaagaau uggagaagcu guuaaaagau     3540

uauucuaucg aguacggcca cggugaaugu auaaaggcag ccauuugcgg ggagagcgac     3600

aaaaaguucu uugcuaagcu cacauccgug cugaacacga uccuucagau gcggaauagc     3660

aaaaccggca cugaacugga uuaccuaauc agcccuguug ccgacgucaa cggaaauuuc     3720

uuugauaguc gacaggcacc caagaacaug ccacaagacg cggaugccaa uggcgcuuau     3780

cacauugggc ucaaaggacu gauguugcug gacagaauca agaacaauca ggaggguaaa     3840

aaguuaaacc ucgugauaaa gaaugaagag uacuucgagu uugugcagaa caggaauaac     3900

uga                                                                   3903


<210>  3469
<211>  3756
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3469
augaaccaca ugaagcaguu caccaaucaa uuuagccugu ccaaaacacu cagauucgag       60

cuuauccccc agggcaagac uaaagaauuu auugagauaa acgggcugau cgaaaaggac      120

aaugagaggg ccgugucuua caaaaagguc aagaaaauua ucgaugaaua ucauaaguac      180

uucauugaga ugguuuugug cgacuuuaaa uuacacggac uggagacgua ugaaaccauc      240

uucaacaaga aagagaagga ugacacagau aagaaagaau uugacaauau acggaacuca      300

cuacgcaagc agaucgcuga ugcauucgcc aaaaauccua acgacgagau uaaggagcga      360

uuuaaaaauc ucuucgcgaa ggaacugauc aaacaggauc uucugaacuu uguggacgau      420

gagcaaaagg aauugguaaa ugaguucaag gacuuuacua ccuacuucac agguuuccau      480

cagaaccgua gaaauaugua uguggcugau gaaaaagcca ccgcaauugc cuacaggcuc      540

gucaacgaga aucugccaaa guuuaucgac aaccugaaaa uauaugagaa gauuaaaaag      600

gacgcuccgg aacucaucag ugauuuaaac aagacucugg uggagaugga agagauuguu      660

cagggcaaaa cacuugacga aaucuucagc uuguccuuuu ucaaucagac ccugacgcaa      720

acugggaucg agcuauacaa cauugugaua ggaggccgga ccgcagauga ggguaagaca      780

aaaaucaagg ggcucaauga auacauuaac acugacuaua aucagaaaca gaccgauaag      840

aagaaaaagc aggccaaauu uaagcaacug uacaaacaga uccuuucuga ccgccacucg      900

gucucauucg uggcugagac auuugaaacc gaugcccagc uguuggagaa cauagaacaa      960

uucuauagca guguacucug uaauuacgag gacgauggac auacgacuaa cauuuuugag     1020

gcgaucaaga aucugaucau uggcuuaaag acauucgacc uguccaaaau cuaucucaga     1080

aacgauaccu cucuuacuga cauuagccag aagcuguuug gcgacugguc cauaaucuca     1140

agugcauuga augauuacua ugaaaaacag aaccccauua gcucuaagga gaaacaggaa     1200

aaguacgacg agaggaaagc caaguggcua aagcaagauu ucaauaucga gacaauccag     1260

accgcucuga acgaaugcga cuccgagauu auaaaagaaa agaauaacaa aaacaucguu     1320

ucagaguacu uugccaagcu cgggcuggau aaagacaaua agauugaucu ucugcagaag     1380

auccaccaca acuauguggu cauuaaagac uugcucaaug aaccuuaccc agagaacauc     1440

aagcugggaa aucagaaaga gcaagugagc cagauaaagg auuucuuaga caguauccug     1500

aaccuaauuc auuuccucaa accccugucu cuuaaggaua aggacaaaga aaaggaugag     1560

uuguuuuauu cccuguucac ggcacucuuu gaacaccuga gccagaccau cucgauuuac     1620

aauaaagugc ggaacuaucu uacacagaag gcuuacucua cugagaaaau caagcugaau     1680

uucgaaaacu ccaccuugcu caaugguugg gacgucaaca aggagccugu uaauacauca     1740

gugauauuuc gcaaaaacgg ccuguucuau uuagggauua ugaguaagag caauaaccga     1800

aucuuugaga gaaacguacc aguguguaaa aaugaagaga cugccuucga aaagaugaac     1860

uacaaacugc uacccggagc gaauaagaug cucccuaaag ucuuucuguc cgccaagggc     1920

auugagucuu uccaaccaag cgcagaaauc cagucaaagu accagaaaga gacccauaag     1980

aaaggugacg cuuuugugag gaaggauaug gagaaccuua uagacuucuu uaaacagagu     2040

aucgccaagc acacagauug gaagcacuuc aaucaucaau ucuccaaaac cgaaacuuau     2100

aacgacuuga gcgaguuuua caaggaaguu gagaaacagg gguauaagcu gacguucaca     2160

aaacucgaug agaccuacau uaaucagcug guggacgaag gaaaguuaua ucuguuucaa     2220

aucuacaaca aggauuucuc uccguuuuca aaaggcaagc ccaauaugca cacucuuuau     2280

uggaaaaugu uguucgacga gcagaaccuc cagaaugucg uguacaagcu gaacggcgaa     2340

gcugagguau uuuuccggca gaguagcauu aaacaaaccg aucguaucau acauaaggcc     2400

aaucaggcaa uugacaacaa gaauccucua aacaacaaaa agcaguccuc uuuuaauuac     2460

gaucugauca aagacaagcg cuucacacuc gauaaauuuc aguuccacgu uccaaucacc     2520

cugaacuuca aggccgaagg gaaugaguau cuuaacacua aggugaauga guacuugaaa     2580

ucgaacagcg acgucaagau uaucggacug gacagaggug aaaggcauuu aauuuaucuc     2640

acacugauaa aucaaaaagg cgagcuucug aagcagcagu ccuugaacgu gaucgcuacg     2700

ucacaggaac acgagaccga uuacaaaaau cuccugguca acaaggaaaa ugagcgggcg     2760

aacgcacgac aagacuggaa aacuauugag acaaucaagg aacuaaagga gggguaucug     2820

agucaggugg uucaccagau ugccaccaug augguggaug aaaaugcuau cguagucaug     2880

gaggaccuca acgccggauu uaugcgcggc agacaaaaag uggaaaggca gguuuaccag     2940

aagcuggaga aaaugcuuau agagaaguug aauuaucugg uguucaaaaa caaugauguc     3000

aacgaaacag ccgguguguu aaacgcacuc cagcugacua auaaguuuga gucuuucgaa     3060

aagaugggga aacaaagcgg cuuucuuuuc uacguacccg cuuggaacac cuccaagauc     3120

gacccugcca cgggauuugu ggauuuccug aaaccaaagu acgagucagu ugagaaagca     3180

aagcuauuuu ucgaaaaguu ugagagcauu aaauucaaug cggacaagaa cuauuuugaa     3240

uuugaguucg auuacaaaaa guucaccgaa aaagcugagg gcucucagac aaaguggacu     3300

gucugcaccc auuccgacgu gcgguaucgc uacaaucccc agacaaaggc cagugaugag     3360

gugaacguca cuaaugaacu caaacugauc uucgacaagu uuaaaauuga guauaagaac     3420

gggaaaaauu ugaagaccga acuguuacuc caggacgaua aacaacuguu cagcaaacuu     3480

cugcacuacu uggcacucac gcugaugcua agacagucua aguccggaac agacaucgau     3540

uuuauacugu caccgguugc caagaacggu guguucuaug acaguaggaa ugcuaugccu     3600

aaccuuccca aggaugccga cgcaaauggc gcuuuucaua uugcccucaa agggcugugg     3660

ugugugcagc agaucaagaa agcggaugac uugaagaaga ucaaacuggc cauuagcaac     3720

aaggaguggu uaucguucgu acaaaaucuc aaauga                               3756


<210>  3470
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3470
augaucgaca acaccaagga gaaaaaggaa ggcagcgugu ucgauggguu uacaagaaaa       60

uaccagcugu ccaagacucu cagguucgag cuucggccca uucugaauac gccuaaaaug      120

uuggacgaug aacaagucau aaagaacgac gagacccgcc gaaagaaaua ugaagccguu      180

aagccauggu uugaucaguu acaccgugag uucaucgagg acgcucugaa aucuuuuaag      240

uucaaaaauc uagcaauuua ccaggauaca uuucagacuu ggcaaaagga cagaaaguca      300

aaacagaaga aagauacccu cgugaagauc gaaguaggac ugagggagga aauugugcgg      360

cgcuucgagg aggucgccaa caucugggug agaagugaac aguauaaacu ucuggguaua      420

aagaaagagg gcuuggggau gcucuuugaa gcgggaguuu ucaggcugcu gaaggagcgg      480

uuuaagaaug aaaaagacac aaccguggau ggcaacaaua ucuucgacga guggacucgc      540

uggacagguu acuuuaagaa auucuucgag acccgaaaga acuuuuauaa aagcgaugac      600

acguccacug cuauugccua cagagucauc aaucagaacc ucaggcgguu cugcgaaaau      660

auucaaaucu uugagaagau aucugaaaag auugaguucu cggaagugga gaaaucauuu      720

gacaucagcu gugcagggau uuucaguuua gccuauuaca acgcuugccu gcuucaggga      780

ggcaucgaua ccuacaauaa gaucauuggc ggggagguag acgaaaaaga uaagaaaaua      840

ccgggaauca acgaguugau uaauaaguau cgucaggaca acuccgguga aaagaucccc      900

uuucugaaac agcuagauaa gcaaauacau ucugcaaaag aggccuucau ugaaagcauc      960

gagacaaaca aggagcucgu uggcaaacug aagacuuuuu acgaaaaugc ugaggugaag     1020

auccaguccu uccgcaaccu uauugccgac aucgucaccg auuauucagg guacgacauu     1080

gauaaaauau aucugacaaa ggaagcggug agucacaaug caagcagaug guuugccucu     1140

uucgaguccu uugagaggga cuuguucgcu guggucgccg aaaaacagaa caagcucguu     1200

uacgagcugu uacggaccca uaaaaaugau ucaaagauca gcgacaaaga cggaaaguuc     1260

aguuuuccug auuucauuaa guguucucac aucaaacgag cacuggaaaa gcaagagggc     1320

cgcaucugga aaggugaaua uuacgaggac auuguggauu uugagaagau aaaagacgua     1380

uucacgcagu uucucugcgu guucaaguuu gaacuugagc agcaguucuu uagaaagacu     1440

acauccgcuc aaaccgggga acagacuaaa aucggauacg agauuuucgu cacaaagauc     1500

aacgaacuga uuaccaggga gaauccagug aucgauuugg aggagaaaau agccaucaag     1560

aacuuugcgg acgccacgcu acugauuuau cagaucgcaa aauacuucgc uguugaaaag     1620

cggcgcggcu ggcucgauaa uuaugaccug gaugacagau uuuacaagag cucggauauu     1680

ggcuaucuua acuucuacag ggacgccuuc gaacagaucg ugcgucccua uaaucuguuu     1740

cggaacuacu ugaccaaaaa gccuuacaau acaaacaaau ggguccucuc uuucgagaau     1800

ccaacucugg cugaugggug ggacaagaac aaagaaaaga ccaacgccgc agugauauua     1860

cgaaaggacg gaagauauua ccuggguauu aucaaagagg auuguaaguc ccuauuugcc     1920

gacagguauu caaaagagau gagugaaggc auugagagcg gguccuucca aaagauggcu     1980

uacaaauuuu uccccgaagc gucuaagaug aucccuaaau gcagcacaca gacuaagaau     2040

guaaaggagc auuuucgcaa aucaaguucc gauuauaacc ucuuccacga gaaggacuac     2100

aaaauaagcg uugcaaucac caagaauauu uaugaacuga acaauguguu uuaccggaaa     2160

gauaacaucg aggaaucuuu cgucccaaag aaugacuuug agaagaaacu uggagugaag     2220

aaauuccaga gacaguacuu ggaaauuuca agggauaaca auggcuauaa gcaagcccug     2280

gcucagugga ucgaguucug uauacgcuuu cucaaagccu acaagaguac aaccauuuuc     2340

gacuauagcc gacugcggga ggcaaaggaa uacgaguccc uugaugccuu uuaucaggac     2400

aucaacgcuc ugacuuacaa uaucucuuuc gucccgauuu cggaacagua uaucaaagag     2460

aagaacgaua auggugaauu guuucucuuc gagauuuaca acaaagacug gagccugggg     2520

cccauggaua agaaucguaa aagaacgaag aacuuacaca cacuguacuu ugagcaacua     2580

uucuccaagg aaaacgagca ggaaaauuuu cucuuccagc ugaacggcga ggccgaacug     2640

uuuuucaggc cuaaaaccga ggagaagcgc uugggauaua aaguguggga cgcaggcgaa     2700

aagaaauggg uuaaggcgaa ggagaaagaa gacggggcug ugauagaucg gaagagauac     2760

gccaaagaca ucauucuguu ucauugccca aucacucuca auaggguauc agagaguaag     2820

accaaacgag agauggaugu cgaaauucgc gaggugcugu cuagcacacc cggaguucac     2880

aucauaggug uggaccgggg cgaaaagcau uuagcauauu acuccgucau cgaucaaaac     2940

gggaaaauua ucgagaccga cacucuuaau ucaauuggaa aggauggcag agguaagccu     3000

guggaauaug ccagcaaacu ugagaagagg gcucaggagc gugaagccuc ucgccgggac     3060

ugggaggaag uagaggcaau caaagauuug aagaaagggu acauauccca ggugauuaga     3120

aaccucgcug accugaucau caagcacaau gccauuauag uuuucgaaga ucuaaacaug     3180

agguucaagc agauccgagg cggaauugag aaaagugcgu aucaacagcu ggagcgggcc     3240

cucaucgaca agcugagcuu ucuugucaaa aagggcgaag aggacccaaa acagacaggg     3300

cacauuuugc gcgcuuacca gcuggcagcc cccgugaucg cuuucaagga uaugggaaag     3360

caaacggguu uaauauuuua cacccaggcc ggcuauacuu cuaaaacaug uccggaaugc     3420

ggguacagaa ggaauaucaa gugucucuuc gagaacauug aacaggcaaa aacccugauc     3480

gagaaucuug aguccauuaa cuauaauaag aaagaagacg uguuucagau cucauacagu     3540

cuggagaagu ugagcucgaa ggaucagaaa aaggagaaaa aggucuccaa cgaacucuau     3600

gcgaaaacac ugaagaaaga cauauucauu cuaacuacca agaaugcccu gcgguacaag     3660

ugguaugauc gauacucuga gaaagcuaag guugccaaaa gaggaaucga cgaauacaag     3720

ggcgagguga acgaaagcga gacgaaaaag ggugugguaa aggaauuuaa ccucaccgag     3780

uaucugaaag ggcuuuugaa gacauacgag aucgauuaug aacauggcgg cauucgcgag     3840

caaauccugu cagucgcaag gggacgugag uucuacaaag acuuuuuaua ugcucucuuc     3900

cugcuuacug aaacccggca caguauaucc gggagaaaua cagauuacau ucagugcccu     3960

gaaugcgagu uugacucuag gaagggauuc aaagauauca aggaguuuaa cggcgacgcc     4020

aauggugcau auaacauugc ccgcaagggg aucaugauac uggaaaagau uaaacaauuc     4080

aaaaaggaua augacggaaa ccuagagaaa augggcuggg gugaccucag caucuccauc     4140

gaagaguggg auaaguucac ucagaaggaa uga                                  4173


<210>  3471
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3471
augaacgccg agaagaaaug cgcuuucaau gaauuuacca accuguacga gcucagcaag       60

acacuuaaau ucgaacugaa gcccgugggc aaaacuaagg agaucauuaa gaauuuuaga      120

gaagaguugg agaaaaacaa uccuauacag uuagacaagg aaaucuccau ugcaaaacaa      180

gagaucaaga aagaucugga cauucuacac agggaguucg uccggcaguc uuuugaacuc      240

gccuucaagg agaacaagau caauuaugaa auauuuuucg agaucuaccg caacuuuaaa      300

aaguucucaa agaauaagaa aagugaugaa uauauugcgg cuaucaagaa ccugaaagag      360

gaaaagauua aacuuguuaa aaagaucgcc gacuacuuug ugaaaaugga ucaggagugg      420

cgaaaguauu acccaagcga gaaaaagucc uuccuguuga ugucuaaugg gguaauaaac      480

cuccugauug acaucaauaa ggaucguaug gacuauucgu uuauuccgaa agaagugugu      540

gagaagauca aaggaaaggg uaaaaucgaa cuguacgaga aguucaacaa gcauuucggc      600

uaccucucaa aauauaauac ggcaagagaa aacuuuuaca gcgaugacgu caagaacagu      660

gccuccgugg cuuauagggc aauuugcgag aauuuagaga aauucuguga uaacaagaua      720

aaaguugaag ccgacaauuu ucccaaggag aucaaggaaa aauuccugga guacuuuaac      780

aagauuaccg aucuuucuuu cuauaaucgg ugcuugagcc agaacgggau cgaagaguac      840

cacaaauuua aggacgagau aaaucgcucc auuaacgaac uguauccuaa uaacaguaaa      900

aauaagccac aaaacauccc cuaccuaagu aaacucgaca agcagauccu gagcgagaag      960

gauaaacagu cuuggauuga agagggauuc aucgacgaga auaacuuuau uuucgaauuu     1020

gagaaguuca uaaaaaugaa ugauaaguac aucccuuccg aaaagaaacu uuuuaacgag     1080

uucuucauuu uucagaacaa ugacgaagug cugcaaaagu uguuccuccu gaaaaagaca     1140

aucaacaauu uugcuaacau cuauuuagau gacccaaaga aucugaugga ucucaugccc     1200

ucaaagggca cuaaaaagag agacuaccuu agccuguugg agauuaagga ggcccuaaac     1260

aguuaugcgg aaaaucagaa cauaggugag acccagaucu ucaaaauuaa guacgaugac     1320

gucaucgaua aaacaaagac cccuuuugaa uguuucauga aaauuuggaa guaugaguac     1380

gaccugcucu auaaugaagg ggacggaaag aaaaacaucg aggagggcug ggaaaagaua     1440

ugcgagaaac ugcagaauaa gaaagaugaa uuuacuggcg acuacgggaa guacaaaaag     1500

gagaucaagg auuauuguga aauggcacuu ucucuguccc aaaggauuaa acauuuggac     1560

auccggauua agaaacgcga uggaagcgag gacgccgaua acaaucucuu cgagggugug     1620

gaaaagcugg acgaguuuua cgaucgauua uucgaaaucg uagacgagca caacgcuuau     1680

caguaccauc acgaguuuca ggccuuccug accaaaaagc cauauaagac agauaaauuu     1740

aagcuaucgu ucgacaaucc gcaacuccug aaaggcuggg aucuuaacaa ggaacccgac     1800

uacuucgggg cauugcuggu uaaaaacgag aaguauuuuc ucgcuguguu ccacaagcag     1860

uacgccaaug aaauauuuag acagaaaaac aaaaauucua cggucaaaau uaaggaggcg     1920

uacgacgugu ucaacggaaa uuauuacaaa aagguggaac ugaagcagau cccuaaacuu     1980

uuuaugaacc ugccaaggau uugcuucgcc aaguccaauc aagaggaguu uggcuugacu     2040

aucgaacucg agaaaauaaa gucagaguuc gauaaauuuc agaagcugaa gaaagaacgg     2100

caggacaaca auuuaaaguu cgaucaggaa aaauuuauca agcugcuagc auauuaccgu     2160

cauugucucg agauucgcga ggacuggaaa gucuucaagu auaacuucaa accccuguac     2220

aaguauagua aucuugguga auuuuacaga gauuuggaga aggacaccua caaccuggau     2280

cucguuaggg ugaauagcga auauguagag aaaaagcgga acgaagggaa acuguacuua     2340

uucgaggugu auaauaagga cuuuuccgau aaaucuaucg gacgaaagaa cauucacagc     2400

gacuacuuca agucacuguu ucuuccugau aacuugacaa auaaaaagau cgcucucgcc     2460

gcugacgccg agauauucuu ucgcgaaaaa ucacugaaga acaaagauga gauuaucacu     2520

caaaagaaua agaucaaaau ugacaagaac aaucauaaag gcgaaaaggc auauuccaaa     2580

aacagauaca ccgacgauaa gaucauucua caucugccaa uaaaucucaa cuucggcgag     2640

aauaagaacc acaaagacuu uaauuaugag aucaacgaua gccugcuuaa gaauaacagg     2700

gacauuaaaa ucauugggau cgaucgggga gaaaaguacu ugcuguacua uucugucaua     2760

gaccagaaag gugauaucaa ggagaugggc gacuucaaug ugguuaacgg gguggauuac     2820

uauucaaaau uagucgccua cgaagagaag cgcaagcagg cuagacucga cuggggagau     2880

auucagagua ucaaagaacu gaaggagggc uauauuagcc acgugaucca cgagauaugc     2940

gaccuuauua aaaaguacaa ugcgaucgua guucuggaag acuuguccau ggguuuuaaa     3000

agggggcguu ucuuucggga gcgacaagug uaucagaagu ucgaacucgc acuguucaga     3060

aagcuaaacu acugggucga uaaaaacaag gaggaaggcg agaaaggauc ugcccugucg     3120

gcuuggcagc ucacacccaa ugagaaggac uuuaaaguga gccagcugga aaaggccagg     3180

caacuuggca ucuuguucua caccgcagcc ggguauacgu ccaagacuug uccggauugc     3240

ggauuucgca aaaacauuac acugaaguac gucaaugaca aacaguuaaa ggaggaucuc     3300

gguaaacuga agauaaacuu cguggaagac cauuuuaaga ucaaauauaa ucuucuggau     3360

uucgagaaga acaaaaaguc agaaaaagcu aaggacaccg uucuaccuaa agaggagcgg     3420

aagauuaguu uuguggccaa uucuaaggua aucagaauua gguggcacga uaucaaaacu     3480

aagcucgcaa aacgcgaacu ggacggcgag aagauaauca aaaaguuccc caaggggguc     3540

auuaaagaau acgauaucaa cauuugcuug aagcgaauca uaggagacaa aauugauuuu     3600

agcaaggacu ccaucacaga gcagcugauc aauggccggu ucgguaaaga cuuuuauugg     3660

gaguuaaugu acuuccucaa ccugauucuu gaaauccgua augugguuuc agagaccgaa     3720

auagauuaua uucaaugucc agugugcggg uuucauagcg acgauggcuu cagaggaaac     3780

aaguucaaug gcgacgcgaa cggggcuuac aauaucgcca ggaagggacg ccugauuuug     3840

gagaaaauca accagcacaa gaaccucaaa gaacugaaga cgggugaucu aucugucaau     3900

auaguggagu gggacaaagc aguaaacaau ugggauaagu uugugcaggg cgacaacacc     3960

gaguccaaga auauugaaau cccugccaaa gaggaaauga ucaguguuga gguccgggaa     4020

agcuga                                                                4026


<210>  3472
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3472
augaagaaag agaaggaguu caagagcuuu ggcgacuuca ccaaccugua cgaaaucucc       60

aaaacacuca gauuugaacu uaaacccgug gagaauacuc agacgaugcu ggaugaagcc      120

gacgucuucg ggaaggauaa gguuauuaaa gacaaguaua ccaaaacaaa gccuuuuaua      180

gauaaauugc acagggaguu cguggacgag ucuuuaaagg auguaucacu gaguggacua      240

aagaaauaca gcgaagugcu cgagaacugg aagaaaaaua agaaagacaa ggauaucguc      300

aaagaacuga agaaggagga ggaacggcuu cgcaaagagg ugguugaauu uuucgacaac      360

acugcuaaga aaugggcaaa ugagaaguau aaagaacugg guuugaagaa gaaagauauu      420

ggcauccucu uugaggaguc cguguucgac cugcugaagg aaaaauacgg ggaggaacaa      480

gauucuuuuc ucaaggagga aaaaggagac uucuuaaaga acgagaaggg cgagaaaguc      540

ucgauuuuug acgaauggaa ggguuucgug ggguauuuca ccaaauuuca ggagacacga      600

aagaauuucu acaaaaacga uggaaccgaa acugcccugg cgacacguau cauagaccag      660

aaucuuaaga gauuuugcga uaacaucgac gauuucaaga aaauuaagaa uaaaaucgac      720

uuuucagagg uagaaaagaa cuucaauaaa accgcugaug uuuuuagcuu ggacuucuau      780

aaccaguguc ugcuacaaaa gggcauugau aguuacaaug aguuuaucgg cgggaaaacg      840

cucgagaacg gaaagaagcu gaaaggugug aacgaacuug ucaaugagua caggcagaag      900

aacaaaaaug aaaagguguc cuuccugaaa uugcucgaca agcagauacu gucugagaag      960

gagaaacuga gcuuuggcau ugaaaacgau gagcaguuac ucgugguccu uaauuccuuc     1020

uaugaaacug ccgaggaaaa gaccaaaauc cugcggacau uguuugggga cuucguugag     1080

cauaacgaga auuacgaccu agauaagacu uauauuucaa aaguggcauu caacaccauc     1140

agucacaagu ggacaaauga aacccauaag uuugaggaac ugcucuacgg agccaugaaa     1200

gaggacaagc caaucggccu gaacuaugau aaaaaggaag acagcuacaa auucccggau     1260

uuuauugcuc uugguuaucu gaagaagugc uugaauaacc ucgacuguga uacgaaauuc     1320

uggaaggaga aauacuacga gaauaacgca gacaagaaag auaaggacaa aggguuucug     1380

acuggaggcc aaaaugccug ggaucaguuc uuacagauau uuaucuucga auuuaaccag     1440

cuguucaacu cugaggcuuu ugacaauaag ggcaaggaaa uuaaaaucgg guaugauaac     1500

uuccgcaagg acuuugagga aauaauuaau caaaaagacu ucaagaacga ugagaaucua     1560

aaaaucgcca ucaagaacuu cgcggacucc guacucugga uuuaccagau ggcaaaguau     1620

uuugccaucg agaaaaagag aggaugggau gacgauuucg aacugucaga guuuuacaca     1680

aaucccagca acgguuauag ucuuuucuac gacagggcuu augaagagau ugugcagaaa     1740

uacaaugauu ugcggaacua ccugaccaag aaaccuuaua aggaggacaa guggaaacuc     1800

aauuuugaaa acccaacucu ggccaauggc uucgauaaga acaaagaguc ugacaauucc     1860

acagucauac uucgcaagaa acgaaaguac uaucuggggu ugaugaagaa aggaaacaac     1920

aagaucuuug aagauagaaa uaaagcagag uucauuagga acaucgaaag cggcgcuuac     1980

gagaagaugg ccuauaaaua ccuccccgac guggcgaaga ugaucccuaa gugcucgacc     2040

caacugaaug aggccaaaaa ccacuuucgu aauucugcag augacuuaga aauuaagaaa     2100

uccuucucaa acccacugaa gauaacgaaa cggaucuuug accuaaauaa cauucaguau     2160

gauaagacca auguuaguaa aaagaucagc ggugacaaca aggggauuaa aaucuuccag     2220

aaggaguacu acaaaauauc cggcgauuuc gacguguaua agucugcucu caaugauugg     2280

aucgacuuuu guaaagauuu ccugagcaag uacgacucaa caaaggauuu ugacuucagu     2340

auucuucgca aaacuaagga uuauaaaucc uuggacgaau uuuacgucga cguggccaag     2400

aucaccuaua aaauuagcuu cacacccgua ucugagucau acaucgauca gaagaacaag     2460

aauggagaac uguaucucuu ugagauauac aaccaagacu ucgcuaaagg caagaugggg     2520

gccaaaaauc ugcauacuuu auacuuugag aacguuuuca guccugaaaa cauuagcaag     2580

aauuuuccaa ucaaacugaa cggaaaugca gagcuuuucu uuagaccgaa guccauugaa     2640

ucuaagaaag agaagaggaa cuucgugcgg gaaaucguca auaaaaagcg auauucggag     2700

gauaaaauau ucuuucacug ccccaucacc uugaaccgcg agacagguag cauuuacaga     2760

uucaauaacu augugaauaa cuuucucucc gaaaauaaca ucaauauuau cggcgucgac     2820

aggggagaga agcaccuggc cuacuauuca gugauagaua aaaacggggu uaagauuggc     2880

ggugggaguu ucaaugaaau caacaaggug gacuacgcua aaaagcuaga ggagcgggcg     2940

ggcgaacgcg agcagucuag aaaagauugg cagguagucg aaggaaucaa ggaccugaaa     3000

aagggcuaua uuagccaggu gguuagggag cucgcagauc uggccaucaa gcauaaugcu     3060

auuauagugc uugaagacuu gaacaugcgu uuuaaacaaa uccgaggggg aauugagaag     3120

uccaucuacc agcagcugga gaaagccuua auugauaagc ucucauuccu ggucgaaaaa     3180

ggugagaagg acccuaacca ggcaggccac auccuuaagg ccuaccaacu ggcugcccca     3240

uuuaccagcu ucaaagauau ggggaagcag acuggaauag uguuuuauac gcaggcaucu     3300

uacacaucca aaaccugucc caauugcggc uuccggaaga acaauaacaa auuuuauuuc     3360

gaaaauaaca ucgguaaggc gcaggacgcu uugaagaaac ucaagacuuu ugaguacgac     3420

agugaaaaua aauguuucgg gcugagcuau ugccuaucug auuucgccaa caaggaggag     3480

guagagaaaa auaagaacaa aaagagaaau aacgcaccuu acuccgacau ugaaaagaaa     3540

gauuguuuug aacugucaac caaggacgcc gugagguauc gcuggcauga uaaaaauaca     3600

gagcggggca agaccuucuu ugaaggagag aguguuuacg aggaaaaaga ggaaaaggag     3660

aucggccaaa cuaagagagg gcucgucaaa gaguacgaca uuagcaagug ccugaucgga     3720

cuuuucgaga aaacaggcuu ggauuauaag cagaaccugu uagacaaaau aaauucgggu     3780

aaguuugaug ggacguucua caagaaccuc uuuaacuauc ugaaucuucu guucgaaauu     3840

aggaacucca ucucuggaac cgaaaucgac uacauuagcu gcccagagug ucaguuucac     3900

acugauaaau caaagacaau caaaaauggc gacgauaacg guaguuauaa uauagcucgc     3960

aagggcauga uuauccuaga caaaauuaag caauucaaga aagaaaacgg gucccucgac     4020

aagaugggau ggggcgagcu guuuaucgau uuggaggaau gggacaaauu cgcccagaag     4080

aaaaauaaca auauaaucga uaaguga                                         4107


<210>  3473
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  3473
augagcaaga acaaugugug ggacaaauuc accaaccagu acucccuguc uaagacacuc       60

agauuugagc uuaaacccgu cggcaagacu agggaaaaga ugaaugagca acugaaguau      120

gauaagcagu ugcagacguu cuuaaaagac caggaaaucg aggaugccua ccaauaucug      180

aagccauuuu ucgacaaacu acacaaggaa uuuauuaccg auucacucga cagugauaca      240

acuaaaaaga uagacuucag cgauuaccug aucgacuauc gggauaagaa agaccuuacc      300

aagaaagagg auucccugcg caagaacauu ggggagguuu uugugaaagc uggagaccga      360

uggaagaaag aaaaguaccc uaaguaugag uggaaaaagg guucuaaugu agcaaacggc      420

ucggggaucu ugcucacaca ggacgugcug augcugauua aagaucucaa uccgaacgac      480

cagaagauca aaaagaugau agaagagacc uucaagggau uuuucacuua cuuuucaggc      540

uucaauuuaa accgugagaa uuacuauaca accaaagucg aaaaggccac ggcgguggcu      600

acuagaaucg uucaugauaa ccugcccaaa uuuugcgaca aucuugugca guucgagugu      660

auuaucaaga aaaagaagaa cggugaaguc gauaggauug agcggaaaag cgaauacuug      720

aaugccuauc aauaccugaa gaguaaaggg cgcaccacac agaucaagga cgcagagucc      780

ggaaaaauga uagaggccga agugauuaac gagaacaugu ucucuaucag cuauuuuucc      840

gcuugccuau cacagagugg cauugaagag uacaauagaa cuaucggcca cuauaaccuc      900

cugaucaauc uuuacaacca ggcaaaggaa ggggaggaga aggaucugaa aaagagcgaa      960

aagaaguuca aaagguugcc uauuuuuaag acccucuaca aacaaauagg auguggugag     1020

aaggaaccac uguucuuuuc uuuaacacau gacaccaagc ugcagguaca ggagaauaaa     1080

gaaaacuaua agaaacccua cuccguugag caaauccucg agcaggccaa acuugcuggc     1140

gauaaauauu ucaaggggaa gucaaaugac ggcauucuga acacgauccc ugaauuuagc     1200

gauuacauau ugcagaaaga gaguuaugga gguguguacu ggucuaaggu cgccauuaau     1260

acuaucucca accgguauuu cgaaaauugg cacacacuaa aagacgcgcu gaucgacgca     1320

aagguguuug agaaagggaa gaagggagau gaggaagacg ugaaaauucc agagagcauc     1380

gaacuccagg gcuucuuuga uguccuggac acccaagagg gcuggaaguc ggaagggauu     1440

uucuuuaaaa agucucuuac ugaggagcug aaaaacaagg aagccgauac aaagaauaaa     1500

aagcgccgag agauaguuaa agaagcuaag agacccuccc aggccuugcu cgcacuguua     1560

uucgacgaua uccaggagca ugcuaaccug uuccuaaauc agucagacga aauucucaaa     1620

cuggagaagu acagugagaa agaaagcaag gagaagauca aacaauggau ggauuccgcc     1680

cuugcgguga acaggaucuu gaaguacuuu uucguaaaag aaucuaaggc caaaggagag     1740

cagauugacc cuauaaucag cgaagcacug aaggugcucu uuaacucaga ggaugcuacc     1800

ugguucaagu gguaugacgc ccugcggaau uaccuuacga aaaagccaca ggaugacguc     1860

aaagagaaca agcugaaauu gaauuuugaa aacaguaccc ucgcuggugg cugggacauu     1920

aauaaggagu ccgauaacua uugcgugauc cugcaggacc aaaaggggaa acagcacuua     1980

ggaauuaucg ccaagaaaga aaagcagcgu ggcuacaaua aaauauucga gcgcacaccg     2040

gagaaccccc uguauaagau cgauagcggu gaaguuuggc agaagaugga guacaaacaa     2100

auugcagugc cuacugggau cggcggauuu gucagaaagu guuucaauac cgcccagcag     2160

uauggcugga aaugcccaga caacugucua aauucugaag ggaagauuau cauaaagaac     2220

gaugaggcua aggaaaaucu cgaggcgauu aucgacugcu acaaggacuu uuucacaaaa     2280

uacgagaagg auggauuuaa auauaagaac uucaauuuua acuucaaaga aucagaggaa     2340

uacgaggaac ugaacaguuu cuuuagcgau guggagaggc aagguuauaa acuuggcuuc     2400

auuacuguaa auaaaaccau cauagacaag uggguugagg augggacaau cuacuuguuu     2460

gaaauuaaga accaggacac caaugauggc aaaaaggacg gacacaaaaa caaucugcau     2520

acuaucuauu ggaaggcacu cuucgaggac guggguaaca aacccaagcu gaaugggggc     2580

gccgaauuau uuuaccggaa ggcucugcuu aaagauaagc aggagaaaau ugaagacaac     2640

uauggaaagg agaucauuaa aaauuuccga uuuuccaagg agaaguucuu guuucacugu     2700

ccuauaaaac ucaacuacaa ggccaaaucu uacucgcauc caaaguaugc acugcccgaa     2760

aucaauaaac agacgaacga guucuuuauc acaauuagcg auaucugcuu ccuaggcauu     2820

gaccgcgggg aaaagcaccu ggccuacuau ucccucguca aucaaaagga ggagauaauc     2880

gaucagggaa cccugaacau gccguucgug gacaaagaug guaagccuag aucaauuaaa     2940

aaggaaaaau uuagguucga cgcuaagguc gauaaauggg ugagugagga aauggacugu     3000

uggaauuaca acgaucuuuu ggacgccaug gcaucuaacc gggacauggc gcgcaagaau     3060

uggcagacua ucggcaccau uaaggagcug aaaaacgggu auaucagcca gguugugaga     3120

aagauaguag auuuagcuau cgacaaaaau aggggcauuc ccuccuuuau cguccucgaa     3180

aaccugaaug ccggauucau gcguggucgg caaaagauug agaaaucagu guaccagaaa     3240

cuugagcugg cauuggccaa gaaacucaac uuuguugugg auaagacagu ccagaauggg     3300

gaaaucggca gcgugaccaa cgcucugcag cuaacuccac cuguaacaaa uuuccaaaac     3360

auagagaauc gaaaacaggu gggaauuaug cuguauacga gagccgacua caccucucag     3420

acugaucccg uuacaggcug gaggaagucc aucuaccuca aaaaggggag ugaagaguau     3480

aucaagagcc aaauuaugga cucuuuugau gacuucggau uugauggcaa aaacuacuau     3540

uucaaguaca ccgucgaccu gauagaucca aucaaaaaga cauccgagaa aaaguuuaag     3600

aaacagugga cucuuuauuc agguauuaau gggaagaguu uggaccgcuu ccggggagaa     3660

agaagcucgg auaaaaacgu guggaucaag acccugcagg acauuaaaac gaccuuagac     3720

gcaguguuua ucggcuucga ucuccagcac ucccugaggu cucaaauaau caaugacggu     3780

auuaugccca agaaaaucga gaacagccag uucacaggcu gggaaucacu ucgcuuugcu     3840

auugagauga uccagcagau acgaaauauu ggggucgaaa agggagauga ggacuucauc     3900

cugaguccug uucgggauaa gaacgaaaac cauuuugacu cccguaaagc caaggagaau     3960

gagccaaacu cuggcgaugc gaauggggcc uacaacaucg cuagaaaagg aauuaucaug     4020

aaugaacaca uaaagaaaga ccuaauuccg uucauccaag auaaggagug ggacgcaugg     4080

cucgccggca gggaagugug ggauaagugg cugauugaga acaaagacag cuugaucuau     4140

aagaaauga                                                             4149


<210>  3474
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3474
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc agacaagaaa       60

uacagcauug gacuggauau agguacaaac uccguuggcu gggccgugau cacagacgag      120

uauaagguac caucuaagaa auucaaggug cucgggaaua cugauaggca uucaauuaaa      180

aagaaccuua ucggagcgcu guuguuugac aguggcgaaa cggcugaggc cacccgguua      240

aaacgcacag cacgacguag auacacuagg cggaagaauc gcauuugcua ucugcaggaa      300

aucuucagca acgagauggc caaggucgau gacuccuuuu uccacagacu agaagagucu      360

uuucucgugg aggaagauaa aaagcaugag aggcacccga uauucgguaa uaucguugac      420

gaaguggcuu accaugagaa auaucccacc auuuaccauc ugcggaagaa acuugucgau      480

ucgacagaca aggcagaucu gcgcuugauc uaucucgccc uggcucacau gauuaaauuu      540

cgagggcacu uccugaucga gggagaccuc aacccugaua auucagacgu ggacaaguua      600

uuuauacaac ugguacagac cuacaaccag cuuuucgaag agaauccaau uaacgccagc      660

ggcguugaug cgaaggcaau cuugagugcc agacugucca aaucuaggcg gcuagaaaau      720

cucauugcuc agcugcccgg cgagaagaaa aacgggcuuu uuggaaaucu gaucgccuug      780

agccucgguc ugacuccuaa cuucaagucc aauuuugacu uagcagaaga ugcuaaacug      840

caacucucaa aggacacaua cgaugacgau cuugacaacc uguuggccca gaucggcgau      900

caguaugcgg accuauuccu ggccgcaaag aaccucagug augcuauucu gcuuagcgac      960

auacugcgug ugaauaccga gaucacgaaa gccccauugu cugcuuccau gauuaagcgc     1020

uacgacgagc accaucagga ucucacucug uuaaaagccc uggucagaca acagcuaccc     1080

gaaaaguaua aagagaucuu cuuugaccag ucaaagaacg gguacgcagg auauauagau     1140

ggcggugcca gccaggaaga guucuacaag uuuauuaaac cuauccucga aaagauggac     1200

gggaccgagg agcugcuugu gaaauugaau agggaagauc ugcuccggaa gcaacgaaca     1260

uucgacaacg gaaguauccc acaccagauu caccugggcg agcuucaugc uauccugcgc     1320

agacaggaag auuuuuaucc guucuugaaa gacaauaggg agaagauuga aaagauacuc     1380

acuuuucgga uccccuacua cgugggcccu cuggcgcgcg ggaacucuag auucgcaugg     1440

augaccagga aauccgagga gacaauuacc ccauggaauu uugaagaggu cguugauaag     1500

ggagccagcg cucaaucguu caucgaacgu augacgaacu uugacaaaaa uuuacccaac     1560

gagaaggugc ugccuaaaca cucucuacuc uaugaguacu ucacuguaua uaaugaacug     1620

acaaagguga aauacgucac cgaggguaug cggaagccag ccuuucuuuc cggcgaacag     1680

aagaaagcaa ucguggauuu gcuguucaag acuaaccgaa aaguuacagu gaagcagcuc     1740

aaagaggacu auuucaagaa gauugaaugu uuugauucag ucgagauaag ugggguggag     1800

gacagauuca augccagccu gggaaccuac caugacuuac ugaaaaucau uaaggauaaa     1860

gacuuucuug auaacgaaga gaaugaagac aucuuggagg auauuguacu cacgcugacc     1920

cuauucgaag acagggagau gaucgaggaa cgccugaaga cauaugcuca ccucuuugau     1980

gacaaaguua ugaagcagcu gaagcggaga agguacacug gcuggggucg ccuuucccga     2040

aaauugauaa acgggauccg ggauaagcaa ucuggcaaaa ccauucugga cuucuuaaag     2100

agcgauggau uugccaaccg uaauuucaug cagcucaucc augacgauuc acugacauuu     2160

aaagaggaca uucagaaggc acaggugagu ggccaagggg acucccuuca cgaacacauc     2220

gcgaaccugg cuggaagccc cgccauaaag aaagguauuu ugcagacugu caaggugguc     2280

gaugagcucg ugaaaguuau gggcagacau aagccggaaa auaucgugau ugagauggca     2340

agggagaacc agaccacaca gaaagggcaa aagaacucuc gcgaacggau gaaaagaauc     2400

gaggaaggaa uaaaggagcu gggcucacag auccuaaagg agcacccugu agaaaauacc     2460

cagcugcaga augagaaacu cuaccuguau uaccuucaaa acgguaggga cauguauguc     2520

gaucaggaau uggacauuaa ucgacugagu gauuacgacg uggaucauau cguuccccag     2580

agcuucuuaa aggacgauuc cauugacaac aaagugcuca cucgcucuga uaagaaucgg     2640

gggaaaucgg acaacguccc aagcgaggaa gugguaaaga agaugaaaaa uuauuggaga     2700

caacugcuua acgccaagcu gaucacgcag aggaaauuug acaaucuaac aaaggcugag     2760

cguggcggac ucuccgagcu ggauaaagcc ggcuucauaa agcgccaguu gguggaaacc     2820

cggcagauua cuaagcacgu ugcacaaauc cuggacucaa gaaugaacac caaauacgau     2880

gagaacgaca aguuaaucag ggaagucaaa gugauuacac ucaagaguaa acuggugucu     2940

gauuuucgaa aggacuucca guucuauaag guccgggaga ucaauaacua ccaccaugcu     3000

cacgaugccu accuuaaugc gguugugggg accgcccuga uuaaaaagua uccuaaauug     3060

gaaagcgagu uuguguacgg agacuauaag guauacgaug uccgcaaaau gauagcuaag     3120

uccgagcagg aaaucgguaa ggcaacugcc aaauauuucu uuuacucaaa cauuaugaau     3180

uucuuuaaga cagagaucac gcucgcuaac ggcgaaauua gaaaaaggcc ccugaucgag     3240

accaaugggg aaacuggaga gauagugugg gacaagggcc gggauuucgc cacaguucga     3300

aaagugcuaa gcaugccaca ggucaacauc gugaagaaaa ccgagguuca aacagguggg     3360

uuuucuaagg aauccauucu gcccaagaga aauagugaca aacuuaucgc acgcaagaaa     3420

gauugggacc cuaagaaaua uggcggauuc gacagcccaa cuguggcgua cucuguacuc     3480

gucguggcca agguggagaa gggcaaaucc aagaaacuga agucagucaa agaauugcug     3540

gggauuacca ucauggagag gaguagcuuu gagaagaacc cgauagauuu cuuagaagcu     3600

aagggauaca aagagguuaa gaaagaccuc auuaucaagc ugcccaaaua uucgcuuuuu     3660

gaacuggaga auggccguaa gcggauguug gccuccgcug gugaacucca gaaggggaac     3720

gagcuggcac uaccuucuaa auacgugaau uuccuguauc uugccagcca uuacgagaag     3780

cucaaaggau caccagaaga uaacgagcag aagcagcugu ucguagaaca acacaaacau     3840

uauuuggacg agaucauuga acagauaagu gaguuuucca agagagugau cuuagcagau     3900

gccaaucugg acaaaguccu cucugcuuac aacaagcaca gggauaagcc cauucgcgag     3960

caggcggaaa acaucauuca ccuguucacg cuuaccaauc ugggcgcccc ugcagcuuuu     4020

aaauauuucg acacaacuau cgaucgaaag agauacacca gcacaaaaga gguguuggac     4080

gccacucuaa uacaucaauc caucaccggu cuguacgaaa cgcggauuga ucucucacag     4140

cugggcgggg acaagaggcc agccgcuacc aaaaaggcag gacaggccaa gaaaaagaaa     4200

uga                                                                   4203


<210>  3475
<211>  4986
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3475
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc aaacuucaag       60

auucugccaa uagccaucga ccucggaguu aaaaauaccg guguguuuag cgcguucuac      120

cagaagggca caucccuuga gaggcuggau aacaagaaug ggaaaguaua ugaauugucu      180

aaggacucau acacuuuacu gaugaacaau cggacggcuc gccgacauca acguagagga      240

auugauagga aacagcuagu gaagcggcuc uuuaaacuga ucuggaccga gcagcuuaac      300

cuggaauggg acaaggauac acagcaagcc auuaguuucu uguuuaaucg cagaggcuuc      360

agcuuuauca cugacgguua uuccccggag uaccucaaca uaguccccga acaggugaag      420

gcaauccuga uggauauuuu cgacgauuau aauggggagg acgaucugga cucuuaccuc      480

aaauuagcca ccgagcagga aucgaagauc ucagagauuu auaacaaacu gaugcagaag      540

auccuugaau uuaaauugau gaagcugugc acagauauaa aagacgacaa gguuagcacc      600

aagacucuaa aagagauuac aaguuacgag uucgaacucc uggcugauua ccuugcaaau      660

uauuccgagu cucugaagac ccaaaaauuu agcuacacgg acaagcaggg aaacuugaaa      720

gaacucuccu auuaccacca ugauaaguau aauauccagg aguuccugaa gaggcacgcc      780

acuauuaacg accggaucuu agauacccug cucacagacg aucuugacau cuggaauuuu      840

aacuucgaaa aauucgauuu ugacaagaac gaggagaaac ugcagaauca agaagauaag      900

gaccauauuc aggcucacuu gcaccauuuc guguuugccg ucaacaaaau aaagucagag      960

auggcgagug gcggccgcca ccgaagccag uacuuccaag aaaucacuaa ugugcuagac     1020

gagaacaauc aucaggaagg guaucugaag aacuuuugug agaaucucca caacaaaaag     1080

uacucuaauc uguccguuaa aaaccuugua aaucugauug gaaacuuguc aaaucucgag     1140

cugaagccuu uaagaaaaua cuucaacgau aagauccacg caaaggccga ccauugggau     1200

gaacagaaau uuaccgagac auauugccac uggauacugg gugaauggag ggugggcguc     1260

aaggaccagg auaaaaagga cggggcuaaa uacagcuaua aggaucuaug uaaugagcuc     1320

aaacaaaagg ugaccaaggc cggacuggug gacuuccuuu uggaacugga uccaugccgg     1380

acgauucccc cuuaccucga caacaacaau cguaaaccac ccaaguguca gagucugauc     1440

cuuaacccua aauuucugga uaaucaguau ccaaacuggc agcaauacuu gcaggagcuc     1500

aagaaacugc agucuaucca gaauuauuua gacuccuucg agacugaucu gaagguccua     1560

aagagcucga aagaccaacc guacuuuguu gaauacaagu cuuccaacca gcagauugca     1620

ucaggccagc gcgacuauaa agaucucgac gcuagaaucc ugcaauucau uuuugauagg     1680

gugaaggcca gugacgagcu uuugcugaau gaaauauacu uccaggcgaa aaagcucaag     1740

cagaaagcca gcuccgagcu ggagaaguua gaaucuagca aaaagcugga ugagguaauc     1800

gcaaacucac aacuuaguca gauuuugaaa ucccagcaua caaaugguau cuucgaacag     1860

gggaccuuuc uccaccuggu gugcaaguau uacaagcaac ggcagcgagc ucgcgacagc     1920

agacuauaua ucaugcccga guacagguau gauaaaaagc ugcauaaaua caacaauacu     1980

ggacgguucg acgaugacaa ccagcuccug acauacugua aucacaagcc ucgccagaaa     2040

agauaucaac uuuugaacga ucuggccggc gucuuacagg ugucuccaaa uuuucucaag     2100

gacaaaauug gcucagauga cgaccuguuc auaaguaagu ggcuuguuga acacaucagg     2160

ggguuuaaga aagcaugcga ggauagccug aagauucaga aagacaaccg uggauugcuc     2220

aaccauaaga ucaauauugc ccggaacacc aaagguaagu gugagaagga aaucuucaau     2280

cugauaugca aaaucgaggg cuccgaagau aagaaaggga acuacaagca cggacuagca     2340

uaugagcugg gcgugcuccu guuuggugaa cccaaugagg ccucuaaacc ugaguucgac     2400

cgaaagauua agaaauuuaa cucgaucuac agcuucgcuc agauucaaca gaucgcguuu     2460

gcagaaagaa aggggaaugc caacacgugu gcugucugcu ccgccgauaa ugcacauagg     2520

augcagcaaa uaaaaauuac cgagccagug gaagacaaca aggauaaaau cauucuuuca     2580

gccaaggcuc agcgcuugcc cgccaucccg acacggauag uagacggcgc aguuaagaaa     2640

auggcgacua uccuggcuaa gaauauugug gaugacaacu ggcagaauau caaacagguc     2700

uuaagugcca agcaccaacu ccacauuccu aucauaaccg agucuaacgc auucgaauuu     2760

gagcccgccc uggcugaugu gaaaggaaag agccuuaagg acagaaggaa aaaggcccug     2820

gagcgcauuu ccccagaaaa uaucuucaaa gacaagaaca accgaaucaa agaguucgca     2880

aagggcauuu cagcuuauag cggggccaau cuaacagaug gagacuuuga uggugcgaaa     2940

gaagaacucg accauaucau uccucggucu cacaagaagu acggcacucu gaacgaugag     3000

gccaauuuga uaugcgucac ccguggggac aacaaaaaua agggaaacag aaucuucugu     3060

cugagggauu uagcugacaa uuauaaacuc aagcaguuug aaacaaccga ugaccuggag     3120

auugagaaaa agaucgcaga uacuauuugg gacgccaaca agaaagauuu caaguuuggc     3180

aauuaccgcu ccuucaucaa ccuuacgccc caggagcaga aggcuuuucg gcaugcccug     3240

uucuuggcag acgaaaaucc aauaaaacaa gcggugauca gagccauuaa caauaggaac     3300

cgaacauuug uuaaugguac ccagcgcuac uucgcugagg ugcucgccaa caauaucuau     3360

cugcgggcua aaaaggagaa ccuaaacacu gacaagauua guuuugauua cuucgggauc     3420

cccaccauag gcaauggaag aggcauugcu gaaaucaggc agcuguauga gaaaguagac     3480

agcgauaucc aggccuacgc aaagggggac aaaccucaag ccucuuauuc ccaccuuauu     3540

gaugcuaugc ucgcguucug cauagccgca gacgaacauc guaacgaugg aucaaucggu     3600

cuggagauug acaagaauua caguuuguau ccacuggaua aaaacacagg cgaagucuuu     3660

accaaggaca ucuucagcca gauuaagauc acugauaaug aguuuucgga caaaaaguua     3720

gugcgcaaaa aggcuauaga gggguucaac acacaccggc agaugacgag agauggaauc     3780

uacgccgaaa auuaccuccc gauucugauc cacaaagagc uuaacgaagu uaggaagggc     3840

uauaccugga aaaauuccga ggaaauuaag aucuuuaagg guaaaaagua cgacauacag     3900

caacugaaca auuuggugua uugucucaaa uucgucgaca agcccauuuc uaucgauauc     3960

cagauuagca cucuggagga gcuacgaaac auccugacaa ccaauaacau agccgcuaca     4020

gcagaauacu auuacauuaa ucuuaaaacu cagaagcucc augaguauua caucgaaaac     4080

uacaacaccg cccuggggua uaagaaauac ucaaaggaga uggaguuuuu gcggaguuua     4140

gcguaucgcu ccgaaagagu gaaaauuaag ucuaucgacg auguaaaaca agugcuggac     4200

aaggauagca auuucauaau uggcaagauc acgcucccuu uuaaaaagga guggcagagg     4260

cuguaccggg aauggcagaa caccacaauc aaagacgauu augaguuccu uaaguccuuu     4320

uucaauguua aaucaauuac uaagcugcac aagaaagucc gaaaggacuu uagcuugcca     4380

aucaguacca acgaaggaaa auuccuagug aagagaaaaa caugggauaa uaacuucauu     4440

uaccagauac ugaaugacuc ugauucccgc gcugacggca cuaagcccuu uaucccugcc     4500

uucgacauua gcaaaaacga gaucguggag gcaaucauug auucguuuac cucaaagaau     4560

auauucuggc ucccaaagaa caucgaacug caaaaagucg acaauaagaa cauuuuugcc     4620

aucgauacgu cuaaaugguu cgagguugaa acacccagug accuuaggga uauugggauc     4680

gcuaccauac aguauaagau cgacaauaac ucccguccga aagugcgggu gaaguuagau     4740

uacguaauug acgaugacag caagaucaau uacuuuauga accauucucu gcucaaaagc     4800

agauauccug auaaggucuu ggagauucug aaacagucca cuaucauaga guucgaauca     4860

aguggauuua acaagacaau uaaagagaug cucggcauga agcuggcagg uaucuacaau     4920

gaaaccagca acaauaagag gccagccgcg acuaaaaagg cugggcaggc caaaaagaaa     4980

aaguga                                                                4986


<210>  3476
<211>  3258
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3476
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc aaagaggaac       60

uacauucugg gacucgacau agguaucaca ucaguuggcu augggauuau cgauuacgag      120

acacgggacg ugauugaugc cggaguacgc cuuuucaaag aagcgaaugu ggagaacaau      180

gaaggccgac guuccaagag aggugcuagg cggcugaagc gcagaaggcg gcaucgcauc      240

cagcgaguca aaaaguuguu auuugacuau aaccugcuaa cugaucacuc ugagcucuca      300

gggauaaauc cauacgaagc cagagugaaa ggacugaguc aaaaacuuag cgaggaggag      360

uucuccgcag cccuguugca ucucgcuaaa aggcggggcg uucacaacgu gaaugaaguc      420

gaagaggaca cgggcaacga gcugucuacc aaggaacaga ucucgcguaa uucaaaggca      480

cuggaggaaa aauauguggc cgagcuccag uuagaacgcc ugaagaaaga uggggaggua      540

agaggaagca uuaacagguu uaagacaagu gacuacguua aagaggcuaa gcagcuuuug      600

aaagugcaaa aggccuauca ucagcuggau caguccuuca ucgacacuua cauugaucua      660

cucgaaaccc ggcgaacaua cuaugagggu ccgggcgaag ggucucccuu uggauggaag      720

gacaucaaag agugguacga aaugcugaug ggccacugca ccuauuuccc ugaggagcuu      780

cgcagcguca aguacgcgua uaaugcagau cuguacaacg ccuugaauga ccucaacaau      840

cuggugauaa cuagagacga aaacgagaaa uuagaauauu acgagaaguu ucagauuauc      900

gaaaacgugu ucaaacaaaa gaagaaacca acacugaagc agauugcuaa agagauccuc      960

gucaaugagg aagauaucaa ggguuacagg guuaccucca cggggaaacc cgaguuuacu     1020

aaccuuaagg uguaucacga cauuaaggau auaaccgccc ggaaagaaau cauugagaau     1080

gcagagcugu uggaccagau cgcuaagaua cuaacaauuu accagucaag ugaagauauc     1140

caagaggaac ugacuaaccu caauagcgag cugacccagg aagagaucga gcagauuucu     1200

aaccuuaaag gauauacagg cacccauaau cuguccuuga aggccaucaa ccucauucug     1260

gacgaauuau ggcacacgaa ugauaaccaa auagcgaucu ucaaucgccu gaaacuugua     1320

ccuaagaagg uggaccucuc acagcagaaa gagauuccaa cuacacuggu cgaugacuuu     1380

auccuuagcc ccgugguuaa gagaaguuuc auccagucua uuaaagugau aaacgccauc     1440

auuaagaaau acggcuugcc uaaugauauc auuaucgaac uggcaaggga gaagaacucc     1500

aaagacgcuc aaaagaugau aaaugaaaug cagaagcgua accggcagac caacgagcga     1560

aucgaggaaa uuaucagaac uacagggaaa gagaaugcca aguaucucau ugaaaagauc     1620

aagcugcaug acaugcagga gggaaaaugu cuuuacagcc uggaagcuau accauuggag     1680

gaucuccuga acaauccguu uaacuaugag gucgaccaca uuauccccag gucggugucu     1740

uucgauaauu ccuucaacaa uaagguauua guuaagcaag aagagaacuc aaagaagggu     1800

aaucgcaccc cuuuucagua ccugaguagc uccgacucua aaauuagcua cgaaacguuc     1860

aagaaacaca uccuaaaccu cgccaagggc aaggggcgga uaucaaaaac caagaaagag     1920

uaucugcuug aggaaagaga uaucaauagg uuuagugugc agaaggacuu cauuaaccgc     1980

aauuuggucg auacacgaua cgcaacucgg ggacugauga accuccugcg uuccuauuuu     2040

agagugaaua acuuagacgu caaagugaaa agcaucaacg gcgguuucac cucuuuucug     2100

aggcgcaagu ggaaauucaa gaaagagcgg aauaaggggu acaaacauca cgccgaagau     2160

gcucuuauua ucgcgaacgc agacuuuaua uucaaggagu ggaaaaaguu ggauaaggcc     2220

aaaaagguua uggaaaauca gauguuugag gagaaacaag cugaaucaau gccagagauu     2280

gaaacagagc aggaauauaa ggagaucuuc aucacucccc aucagauuaa acacaucaag     2340

gacuuuaagg auuacaaaua uagucauaga guggacaaga aaccuaacag ggagcucauu     2400

aaugacaccc uguacagcac acgaaaggau gacaaaggca acacccuaau aguaaauaac     2460

cugaauggac ucuacgauaa ggacaacgau aagcugaaaa agcuaaucaa uaaaucccca     2520

gaaaaguugc ugauguauca ccacgacccc cagacuuacc aaaaauuaaa gcucauuaug     2580

gagcaguaug gcgaugaaaa gaacccgcug uacaaauauu acgaggaaac ggggaauuau     2640

cuuacaaagu acucuaaaaa ggacaacgga ccugucauca aaaagauuaa guacuauggu     2700

aauaaacuga acgcccauuu ggauaucacc gacgauuacc ccaacucgcg caauaaggug     2760

guuaaacuca gccugaagcc auaucgguuc gacguguacc uagauaacgg cgucuauaaa     2820

uucgugacug uaaagaaucu ggacgugauc aaaaaggaga acuacuauga gguuaauucc     2880

aagugcuacg aagaggccaa aaagcucaaa aagauaucaa accaggcaga auuuauugcu     2940

aguuucuaca auaacgaccu gaucaaaauu aauggggagc uuuauagagu caucggagug     3000

aacaaugauu ugcugaacag gauagaggug aauaugauug acaucaccua ccgugaauau     3060

uuagagaaca ugaaugauaa gcgcccuccc cggaucauua agacaauagc cucuaaaacc     3120

caaagcauca agaaauacuc cacugacauu cucggcaacc uguaugaagu caagagcaaa     3180

aagcacccac agaucauuaa gaaagguaag agacccgcag cgaccaaaaa ggcugggcag     3240

gccaaaaaga agaaauga                                                   3258


<210>  3477
<211>  4326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3477
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc acuguucaac       60

aagugcauua uaaucagcau uaaucucgac uuuuccaaca aagagaagug uaugaccaag      120

ccauacucua ucggacuuga uauugguaca aauucaguug gcugggccgu gaucacugac      180

aacuauaaag uaccgaguaa gaaaaugaag gugcugggga auacgagcaa aaaguacaua      240

aagaaaaacu uguuaggagu ccugcuauuc gauuccggca ucaccgcgga agguaggcgg      300

cucaagcgca cagcucgacg uagauauacu aggcggcgca auaggauucu guaccuucag      360

gagaucuuuu cuaccgaaau ggccacacug gacgaugcau ucuuucaaag auuggacgau      420

ucguuccucg ugcccgacga uaaacgggac ucaaaguauc cuauuuuugg gaaccugguu      480

gaggaaaaag uguaccauga ugaguuccca accaucuauc aucugcgcaa guaccucgcc      540

gacagcacua aaaaggcuga uuuacgacug gucuaccuug cauuggccca caugauaaag      600

uauagaggac acuuucugau ugagggcgag uucaauagua aaaacaauga cauccagaag      660

aacuuucagg acuuccuaga uacauacaau gcuauuuuug aguccgaccu cucucuggaa      720

aacagcaaac agcuugagga gaucgugaag gauaaaaucu ccaagcugga aaagaaagac      780

aggauuuuga aacucuuccc cggcgagaaa aauucaggga uauucaguga auuucugaag      840

uuaaucguag gaaaccaagc cgauuuccgg aaaugcuuua accuggacga gaaggcgagc      900

cuccauuucu cuaaggaauc cuaugaugag gaccuugaga cccuguuggg uuacauuggc      960

gaugacuauu cagauguuuu ucuaaaagca aagaaacugu acgacgccau ccuccugagc     1020

ggguuccuua cggugacuga caaugaaacc gaggcuccuc ugaguucugc caugauaaag     1080

cguuauaacg aacacaaaga ggauuuggca cuccugaagg aauacauucg caauaucucc     1140

uuaaagacau acaacgaggu cuuuaaagac gauacuaaga auggauaugc uggcuacauc     1200

gacgguaaaa ccaaccagga ggauuucuau guguaccuga agaaucuacu cgccgaauuu     1260

gagggggcgg acuauuuccu ggaaaagauu gauagagagg acuuucuuag gaagcagcgg     1320

acauucgaua acggaagcau cccauaccag auucacuugc aagaaaugcg agccauacug     1380

gacaaacagg caaaguuuua ucccuuccuc gcuaagaaua aagagcgcau cgagaagauu     1440

cugaccuuca gaaucccuua cuacgugggc ccacuugcca ggggcaacuc ggauuuugcu     1500

uggucuaucc ggaaacgcaa ugaaaagauu acgccgugga acuucgagga cgucauagau     1560

aaagaauccu cagccgaggc auuuaucaau agaaugacaa guuucgaccu guauuugccc     1620

gaggaaaagg uucucccuaa gcauagccug uuauacgaga cuuuuaacgu guauaaugaa     1680

cugaccaaag uaagguucau ugccgagucc augcgugacu accaguuucu agauucuaag     1740

cagaaaaagg acaucgugcg gcucuauuuc aaagauaagc gaaaggucac ugacaaagau     1800

auuaucgaau accugcacgc uauauauggg uacgacggaa ucgagcuuaa ggguauugag     1860

aaacaauuua acagcucauu gaguacauac caugaucugc ucaacaucau uaaugacaag     1920

gaguuccugg augacuccag caacgaagcg aucauagaag agauuaucca caccuuaacg     1980

auuuuugaag auagagagau gaucaaacag aggcugucua aguucgagaa uauauuugac     2040

aagucagugc uuaaaaaguu gagucgccgg cacuauaccg gcugggggaa acucagcgca     2100

aagcugauca acggaauuag agaugaaaaa uccggcaaua caauccuaga cuaccugauu     2160

gacgauggua ucucuaacag gaauuucaug cagcucauac augacgaugc ccugucguuc     2220

aagaaaaaga uucaaaaggc ucagaucauc ggggacgagg auaaaggcaa cauuaaggaa     2280

guugugaaaa gccuuccagg aucccccgcc aucaagaaag gcauuuugca gucaauaaag     2340

aucgucgacg agcuggugaa gguaauggga gggcgcaaac cugaaaguau uguuguggag     2400

auggcacgag agaaucagua uacuaaccaa gguaagucua auagccagca gcgguuaaaa     2460

cgucucgaaa agucccugaa agagcuuggc ucaaagaucc ugaaggaaaa cauuccagcc     2520

aaauugagca agaucgauaa uaacgcucuc cagaaugaca gacuguaccu auauuaccug     2580

caaaacggga aagauaugua uaccggagac gaucucgaca uagacaggcu gucuaacuac     2640

gauaucgacc acauuauccc ccaggccuuu cuuaaggaua auuccauuga caacaaaguc     2700

uuggugagua gcgcaucuaa ucgcggcaag uccgaugacu ucccgucacu ggaggucgug     2760

aagaaacgga agacauuuug guaccaguua cucaaaagua agcugaucag ccagagaaaa     2820

uucgauaacc uuacuaaggc ggagaggggu gggcugcuac cugaagacaa ggcuggcuuu     2880

auacaacgac agcucguuga gacccgccag auuacaaaac auguggcccg gcuguuggau     2940

gaaaaguuca auaacaaaaa ggacgagaau aacagagcag uaaggaccgu caaaaucauc     3000

acucugaagu cgacguuagu gucccaguuu cguaaagauu ucgaacucua uaagguucgc     3060

gagauuaaug acuuucacca ugcccacgau gcuuaccuga acgccgugau agcaucugcc     3120

cuucugaaga aauaucccaa guuggagcca gaguucgucu acggagacua uccuaaauac     3180

aauagcuuuc gggaaagaaa gucagcuaca gaaaaagugu auuucuacag uaacaucaug     3240

aauauuuuca agaaguccau cucucucgcg gacggcaggg uaauugagcg accccugauc     3300

gaagugaacg aggagaccgg ggaaagcguu uggaauaaag aguccgaucu agccacuguc     3360

cggcgcgugc ugucauaccc acaagugaac gucguuaaga aaguggaaga acagaaccac     3420

ggacuugaca gagguaagcc caaaggccuc uuuaaugcua accugagcag uaagccuaag     3480

ccaaauucua acgaaaauuu ggugggggca aaagaguauc uggauccgaa gaaauacgga     3540

ggcuaugccg guauauccaa cagcuucgcu guauuaguca aggggaccau cgagaaaggc     3600

gccaagaaga aaauuacaaa ugugcucgaa uuucagggaa ucucgauucu ggacaggauc     3660

aacuaccgga aggauaaacu uaauuuccug uuggagaagg gcuauaaaga cauagaacuc     3720

auuaucgagc ugcccaagua cucacuauuu gagcugucug augggagucg aagaaugcuu     3780

gcauccaucc ucagcaccaa caauaaacgc ggagaaauuc auaagggcaa ccaaaucuuc     3840

cugucucaga aguuuguuaa auuguuauau cacgcgaaga ggauaagcaa uacuauuaac     3900

gagaaucauc guaaauacgu ggaaaaccac aagaaagagu ucgaggaacu guuuuacuau     3960

auccucgagu ucaaugaaaa cuacgucggu gccaagaaga acgggaaacu gcuuaauucc     4020

gcuuuucagu cauggcagaa ccacaguauu gacgagcugu guagcucuuu caucggaccu     4080

acaggcuccg aacggaaggg uuuguuugag cuaacgucaa gaggcagugc cgcagauuuc     4140

gaguuccugg gggugaaaau accaagguau cgcgacuaca cccccagcuc ccuccugaag     4200

gaugcuacuc uuauucauca aucuguuaca ggauuauaug aaacccgaau cgaccuggcc     4260

aaacucggcg aggggaagag accugcagcc acaaagaaag cuggacaggc gaagaaaaag     4320

aaauga                                                                4326


<210>  3478
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3478
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc augguacgcc       60

agccugaugu ccgcgcauca ccucaggguu ggaauugacg uggguaccca uucuguaggc      120

cuugcuacac ugcgggugga ugaccacggg acuccaauag aguuguuauc agcccugagu      180

cauauccacg auagcggagu cggcaaggaa gguaaaaagg accacgauac gcgcaagaaa      240

cuauccggga uugcacgacg ugccagaagg cuccugcauc accggcgcac ccagcuucaa      300

cagcuggacg agguguugag agaucucgga uucccgaucc ccacaccugg cgaauuucug      360

gaccugaacg agcagacuga uccauauagg guuuggcggg ugcgcgcucg acucgucgaa      420

gagaaguuac ccgaggaacu gagaggcccu gcaauuucua uggccgugag gcauaucgcu      480

cggcaccgug gguggcgcaa uccauacucg aaaguagagu cacuuuugag ccccgccgaa      540

gagaguccuu ucaugaaggc gcugagagag aggauacuag caaccacagg agaaguucuc      600

gacgauggua ucaccccagg ccaggccaug gcucaagugg cccugacuca caacauuucc      660

augcgggggc cggagggaau ccuuggcaaa cugcaucagu cugacaaugc aaacgaaauu      720

cgaaagaucu gcgcucgcca gggugucagc cccgaugugu guaagcaguu gcucagagcc      780

guguuuaaag cggacucccc uaggggguca gccgucaguc ggguugcacc agacccccug      840

ccuggacaag gcagcuuccg cagagcucca aagugcgauc ccgaguuuca gagguuccgu      900

auaauuucua ucguggccaa uuuacggauu uccgaaacaa aaggcgagaa ccgaccgcug      960

accgcugacg agagaaggca cguagugacg uuucucacug aagauucaca ggccgaccuu     1020

accugggucg auguggcaga gaagcugggg guucaucgcc gggacuugag aggaacagcc     1080

gugcacacug augacgguga aaggagcgcu gcgcgcccuc ccaucgaugc aaccgaccga     1140

aucaugcggc agacaaaaau uaguucucua aagaccuggu gggaggaagc cgauuccgag     1200

caacguggcg cuaugauaag auaucuguac gaggacccaa cggacagcga augugccgag     1260

aucauugcag aacucccuga ggaagaucag gccaaacugg acucgcuuca ucugcccgcu     1320

gggagggccg cauauucucg cgaguccuug acugcgcucu cagaucacau gcuggcuaca     1380

accgacgauu uacacgaggc ccggaagaga cuguucggag ucgacgauag uugggcacca     1440

cccgccgaag cuaucaaugc cccugugggc aacccaagcg uagacaggac ucuaaagaua     1500

guuggucgau accucuccgc aguggagucu auguggggga caccggaagu cauucaugug     1560

gagcacgucc gcgauggcuu uaccagcgag cggauggcug acgaaagaga uaaagccaau     1620

aggcgucgcu auaacgacaa ucaggaggcg augaagaaaa uccaacggga uuacggaaag     1680

gaaggcuaca ucucaagagg ggacauugug aggcuggacg cccuugaguu gcagggaugc     1740

gcuugucugu auugcgguac gaccaucggc uaccauacau gucagcucga ucacauuguu     1800

ccccaggcag ggccuggaag uaacaaucga cggggcaacc ugguggccgu augcgaacgc     1860

uguaauagau ccaaaagcaa cacuccauuc gcugucuggg cccaaaagug cgguauaccc     1920

cacguggggg uuaaggaggc aaucggcagg gugcggggau ggcgaaaaca gaccccuaac     1980

acaucuucag aggaccuuac uagacugaag aaagaaguca uugcgcgcuu gaggcguacc     2040

caggaggauc cagaaaucga cgagcggagu auggaaagcg uggccuggau ggcuaaugag     2100

cuccaucaca gaaucgccgc agcuuauccc gagacaaccg uaauggugua caggggcucc     2160

auuacugccg cagcccgcaa ggcugcgggg auagauucuc gaaucaaccu gauuggagaa     2220

aaagguagaa aggaccggau cgauaggcgc caucacgccg uugacgcauc ggucguggcu     2280

uuaauggagg ccagcguggc caagacgcug gcugaacguu ccucacuaag aggcgagcag     2340

cggcucacag ggaaagaaca aaccuggaag caguauacug gaaguaccgu cggcgcaagg     2400

gagcauuuug agauguggcg cggucacaug cugcaccuua cagaauuguu caaugagcga     2460

cuggccgaag auaaaguuua cgugacccag aacauuagac uccggcuguc ugacgggaau     2520

gcgcauacug ugaacccgag caaguuagua ucccacaggc ugggcgaugg acuuacaguc     2580

cagcaaaucg accgcgcuug uacgccugcc uuguggugcg cacucaccag agagaaagau     2640

uuugacgaga agaauggccu gccagccagg gaagaccggg cuauacgugu gcaugggcac     2700

gagaucaagu caagcgauua uauucagguu uucucuaaac gcaagaaaac ugacuccgau     2760

cgagacgaaa cacccuucgg agcaaucgcc gugagaggcg guuuugucga gauugggccu     2820

aguauccauc acgcgaggau auaccgggug gaaggaaaga aacccguuua cgcuaugcua     2880

cgcguguuca cccacgaucu gcucagccag agacauggcg accuguuuuc ugccguaauu     2940

ccaccucagu ccaucucaau gaggugcgca gagcccaaac uucggaaagc cauuacaacu     3000

gguaacgcua ccuauuuggg cugggucgug gugggggaug agcuggaaau caaugucgac     3060

aguuucacga aguacgccau aggacgauuu uuagaggauu ucccaaacac cacaagaugg     3120

cgcaucugug gcuaugacac uaauagcaag cucacccuga aaccgauugu ucuugcagcu     3180

gaagggcugg agaacccuuc guccgccgug aaugaaaucg uagaguugaa gggauggagg     3240

guggcgauua acguccucac aaaagugcac cccacuguug ugcggcguga ugcccugggc     3300

agaccaaggu acucuagccg cucaaaucua cccaccaguu ggacgaucga gaagcggccu     3360

gcagcuacaa agaaggccgg ucaagcuaag aaaaagaaau ga                        3402


<210>  3479
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3479
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc aaccaagcca       60

uacagcauug gacuggacau agguacaaac uccguuggcu gggccgugac uacggauaau      120

uauaaaguac cgucuaagaa gaugaaagug cucgggaaca ccucaaagaa auacaucaag      180

aaaaaucuuc ugggagucuu guuauucgac aguggcauua cagcggaggg uaggcggcug      240

aagcgcacug cucgacguag auauaccagg cggcgcaaca gaauccuaua ccuccaggaa      300

auuuuuagca cagagauggc cacccuggau gacgcauucu uucaaaggcu ugaugacucc      360

uuccuggugc ccgaugacaa gcgggauucu aaauauccua ucuuugggaa uuugguugaa      420

gagaaggccu accaugacga guucccaacu auauaucacc uccgcaaaua ccuggcugau      480

ucgacaaaga aagcagaccu gcgacucgug uacuuagccc uggcucauau gaucaaguau      540

agaggacacu uucuuauuga gggcgaguuc aacucaaaga auaacgauau ccagaagaau      600

uuucaggacu ucuuggacac cuacaacgcc auuuuugaaa gcgaucugag ucuagaaaau      660

uccaagcagc ucgaagagau cgucaaagac aagauaucua aacuggagaa gaaagauagg      720

auucuuaagc uguuccccgg cgaaaagaac agcgggaucu uuuccgaguu cuugaaacuc      780

auugugggaa aucaagcgga cuuccggaag ugcuuuaacc uggaugaaaa agcaucauua      840

cauuucagua aggagagcua ugacgaagau cuggagacgc uccuugguua caucggcgac      900

gauuauucug acguauuucu gaaagccaag aaguuguacg augcuauccu acuguccggg      960

uuccucacug uuaccgacaa ugagacagaa gccccucugu caagcgcaau gauuaaacgu     1020

uauaacgagc acaaggaaga ccuugcucug uugaaagagu acauacgcaa caucagucuc     1080

aagacuuaca augaaguguu uaaagaugac accaagaacg gauaugccgg cuacauugau     1140

gguaagacaa aucaggagga cuucuauguc uaccugaaaa aguuacuggc ggaguuugaa     1200

ggggccgauu auuuccuaga gaaaaucgac agagaagauu uucucaggaa gcagcggacc     1260

uucgacaacg gaucuauacc auaccagauu caccuucaag agaugcgagc aauccuugau     1320

aaacaggcua aguuuuaucc cuucuuggcc aaaaauaagg aacgcaucga gaagauucug     1380

acguuuagaa ucccuuacua cgugggccca cucgcuaggg gcaacuccga cuucgccugg     1440

agcauucgga aacgcaauga gaagauaacu ccguggaacu ucgaagaugu gaucgacaaa     1500

gagucgucug cagaagccuu uauuaauaga augacauccu ucgaucugua ucuucccgag     1560

gagaaggucc ugccuaaaca uucauugcuc uacgaaaccu uuaacguuua uaaugagcug     1620

acuaagguga gguucaucgc ugaaaguaug cgugacuacc aguuuuuaga cagcaagcag     1680

aaaaaggaua ucguacggcu guauuucaaa gacaagcgaa aagugacaga uaaggacauu     1740

auagaguacc uacacgcgau cuauggguac gauggaauug aacucaaggg uaucgagaaa     1800

caauuuaacu ccucucugag caccuaccau gaccuuuuga auauuaucaa cgauaaggag     1860

uuccuggacg auucaaguaa ugaagcaaua aucgaggaaa uuauccacac gcucaccauu     1920

uuugaggaca gagaaaugau caaacagagg cuguccaagu ucgagaacau auuugauaaa     1980

agcgucuuaa agaagcuguc ucgccggcac uauacaggcu gggggaaacu uucagccaag     2040

uugauuaacg gaaucagaga cgagaaaagu ggcaauacua uucucgauua ccugaucgac     2100

gacgguauaa gcaacaggaa uuucaugcag cuaauccaug augacgcucu guccuuuaag     2160

aaaaagauuc aaaaagccca gaucauuggg gaugaagaca agggcaacau caaggaggug     2220

guuaaaucuc ucccaggauc gcccgcaaua aagaaaggca uucugcagag caucaagauc     2280

guggaugaac uugucaaagu gaugggaggg cgcaagccug aguccauugu aguugaaaug     2340

gcccgagaga aucaguauac caaccaaggu aagucaaaua gucagcagcg guugaaacgu     2400

cuggagaagu cuuuaaaaga acucggcagc aagauccuga aagagaacau uccagcuaag     2460

cuguccaaga uagacaauaa cgcccugcag aaugauagau uguaccucua uuaccugcaa     2520

aacgggaaag acauguauac aggagaugac cuagauaucg acaggcuguc aaauuacgac     2580

auugaucaca ucauucccca ggcauuccuc aaggacaaca gcaucgauaa uaaagugcug     2640

gucucuuccg cgaguaaccg cggcaagagc gacgaugugc cgucucuuga agucgugaaa     2700

aagcggaaga cuuucuggua ccaguugcug aaauccaagu uaauaucaca gagaaaauuu     2760

gacaaccuca ccaaggcuga gagggguggg cugaguccug aggauaaagc cggcuucauc     2820

caacgacagc uuguugaaac acgccagauu accaagcaug uggcacggcu gcuagacgag     2880

aaguuuaaua acaaaaagga ugaaaauaac agagccguaa ggacugucaa aaucauuacg     2940

cucaagagca cacugguguc gcaguuccgu aaagacuuug aguuguauaa gguucgcgaa     3000

aucaaugauu uccaccaugc ucacgacgcc uaccugaacg caguggucgc uuccgccuua     3060

cucaaaaagu aucccaagcu ggagccagag uuuguguacg gagauuaucc uaaauacaau     3120

ucuuuccggg aaagaaagag cgcgaccgag aaaguauauu uuuacucaaa cauaaugaau     3180

auuuucaaga aaaguaucuc ccuugccgac ggcaggguga ucgaacgacc ccugauugag     3240

guuaacgaag agacugggga gucugucugg aauaaggaaa gcgacuuggc uaccgugcgg     3300

cgcgugcucu ccuacccaca agucaacguu gugaagaaag uggaggaaca gaaucacgga     3360

cuggauagag guaagcccaa aggccuauuu aacgcaaauc ugucaagcaa gccuaaacca     3420

aacaguaacg agaaucuugu aggggccaag gaauaucucg acccgaagaa auacggaggc     3480

uaugcuggua uaucuaacuc cuucacaguc cuggugaagg ggaccaucga gaaaggcgcc     3540

aagaaaaaga uuacuaaugu uuuggaguuc cagggaauca gcauucugga uaggaucaac     3600

uaccggaagg acaaauuaaa uuuucuccug gaaaagggcu auaaagauau agagcuuauc     3660

auugaacugc ccaaguacuc guuguucgag cucucagacg ggucucgaag aaugcuggca     3720

aguauccuau ccacaaacaa uaaacgcgga gagauucaua agggcaacca aaucuuucug     3780

agccagaaau ucgugaaacu ucucuaucac gcgaagagga uaucuaauac gauuaacgaa     3840

aaucaucgua aauacgucga gaaccacaag aaagaauuug aggaacuguu cuacuauauc     3900

uuggaguuua augagaacua cgugggugcc aagaaaaaug ggaaguuacu gaacagcgcu     3960

uuccaguccu ggcagaauca cucaaucgau gaacucugua guagcuuuau uggaccuacc     4020

ggcucugagc ggaagggucu guucgaacuu acuuccagag gcucagccgc agacuuugag     4080

uuccuggggg uuaaaauccc aagguaucgc gauuacacac ccaguagcuu gcuaaaggac     4140

gcuacccuga uacaucaauc cgugacagga cucuaugaaa cucgaauuga ucuggccaaa     4200

cuuggcgagg ggaagagacc ugcagccacc aaaaaggcug gacaggcgaa gaaaaagaaa     4260

uga                                                                   4263


<210>  3480
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3480
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc agccgcguuc       60

aagccaaaca gcauuaauua cauacuggga cucgacaucg guauugcuuc cguuggcugg      120

gccauggugg agaucgauga agaggaaaac ccgauuaggc uuaucgaccu ggggguacgg      180

guguuugagc gcgcagaagu ccccaaaacc ggagauucuu uggccauggc ucgacguuua      240

gcaagaucag ugaggcggcu gacacgcaga agggcccauc ggcuacuccg cacucgaaga      300

cugcuuaaga gggagggcgu ucugcaggcu gccaauuucg acgagaacgg uuugauaaag      360

agucucccua auacgccaug gcaacugcgg gcggcagccc uggaucguaa acucaccccc      420

uuagaaugga gcgcugugcu gcuucacuug aucaagcauc gcggguaucu gucccagagg      480

aaaaacgagg gagaaacagc cgacaaggag cuaggcgcac uccugaaagg cgucgcuggg      540

aaugcccacg cgcuucagac uggagauuuu aggaccccug ccgagcuggc auugaacaag      600

uucgaaaagg agucugguca uauucggaau cagcgaucgg acuacucaca cacauuuagc      660

cgcaaagauc uccaagcuga acugaucuua cuguucgaga agcagaaaga auuuggcaac      720

ccacacguga gugggggacu caaggagggc auugagaccc uucugaugac ucagagaccc      780

gccuuguccg gugacgcugu acagaaaaug cuagggcauu gcacauucga accugccgag      840

ccaaaggcag ccaaaaauac cuauacggcu gaaagguuua ucuggcugac uaagcucaac      900

aaucugcgga uacuugagca aggaucugaa cgcccgcuga ccgauacaga gagagcgacu      960

uugauggacg agcccuacag aaagagcaaa cucaccuaug cacaggcccg uaagcuguua     1020

ggccuggaag auacagcuuu cuuuaaaggc cuacgguacg ggaaggacaa cgccgaggca     1080

uccacccuca uggaaaugaa agccuaucac gcuauuucac gagcccugga gaaggaagga     1140

cuuaaggaca aaaagagucc uuugaaucug agcccagagc uccaggauga gaucgguacg     1200

gcauucucuc uguuuaaaac ugacgaagau auuacaggca gacuuaagga caggauccag     1260

cccgagaucc uggaagcguu gcucaaacau auuuccuucg auaaguucgu ucaaauauca     1320

cugaaggcuu uacgccggau cgugccucug auggagcagg ggaaaagaua cgacgaggcc     1380

ugugcagaaa uuuacggaga ucacuauggc aagaaaaaca ccgaggaaaa gaucuaccua     1440

ccacccauac cggccgacga gauuaggaac ccugucgugc uccgcgcucu gagccaggcc     1500

cgaaaaguga ucaauggugu cguucggcgu uaugggaguc ccgcaagaau ccacauugaa     1560

acugcuaggg aggugggcaa gucuuuuaag gaucgcaaag agaucgaaaa gcggcaagag     1620

gaaaacagaa aagacaggga gaaggccgcg gccaaauucc gagaauacuu uccaaauuuc     1680

guaggagagc cuaaguccaa agauauucuu aaguugcgcc uguaugagca gcagcauggc     1740

aagugccucu acagcgggaa agaaauaaac cugggacggu uaaaugagaa ggguuaugug     1800

gaaaucgacc acgcucugcc cuuuucgaga acaugggacg auucuuucaa caauaaaguc     1860

cuuguguugg gcuccgagaa ccagaauaag gggaaccaaa ccccauacga auacuuuaau     1920

ggaaaagaca acucaaggga guggcaggag uucaaggcac guguugaaac gagucgcuuu     1980

ccccggagca agaaacagag gauucuccug cagaaguucg augaggacgg cuuuaaagaa     2040

agaaaucuaa acgauacccg auaugugaau cgguuccugu gucaauuugu cgccgaccgc     2100

augagacuca cagguaaggg gaaaaagagg guguucgcuu ccaacggcca gaucacuaac     2160

cugcuucggg gauucugggg cuugcgaaag guaagagccg agaaugaucg ccaucacgca     2220

cuggacgcgg uuguggucgc cugcucuacc guggcuaugc agcagaaaau cacaagguuu     2280

guccguuaca aggagaugaa cgccuucgau gggaaaacua uugacaagga aaccggagag     2340

guguuacauc agaaaacaca cuuuccucag ccaugggagu ucuuugcaca ggaaguuaug     2400

auacgggugu ucgguaagcc ggauggcaag cccgaauuug aggaggcuga cacccucgaa     2460

aaacugagaa cucuucuggc cgagaaguug agcucaaggc cugaagcagu acacgaguau     2520

gucacgccac ucuucgugag ucgcgccccc aaucgaaaaa uguccgggca gggacauaug     2580

gaaacaguua agagcgcuaa aagacuggau gagggcgugu cuguccuacg ggugccucug     2640

acccaacuca agcugaaaga ccuugagaag augguaaaca gggaacgcga gccaaaguug     2700

uacgaagcgc ugaaagcccg uuuagaggca cacaaggacg aucccgcuaa agccuuugcc     2760

gaaccguucu auaaguacga caaagcuggu aauagaacuc aacaggugaa ggcaguucgg     2820

gucgagcaag ugcagaagac cggggugugg gucaggaacc auaauggcau cgccgauaac     2880

gcgacaaugg uucgcgugga cguguuugag aaaggagaua aguauuaccu cguaccuauu     2940

uacucauggc aggucgcuaa aggcauccug ccagaccgag ccgugguuca ggggaaggau     3000

gaagaggacu ggcaacuuau ugaugacagu uucaauuuua aauucagccu gcaccccaac     3060

gaucuagugg aagucaucac caagaaggca agaauguucg gauauuuugc cuccugucac     3120

cggggcacug guaauauaaa caucaggauu caugaccucg aucacaaaau cgggaagaau     3180

ggcauucugg agggaaucgg ugugaaaaca gcuuugucuu uccagaagua ccagauagac     3240

gagcugggca aagaaauucg cccuugcaga uuaaagaaga ggcccccagu ucggaaacgu     3300

ccugcagcca cgaagaaagc ggggcaggcu aagaaaaaga aguga                     3345


<210>  3481
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3481
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc aaagaaacca       60

uacaccauug gacuggacau agguacaaac agcguuggcu gggccgugcu cacugaucag      120

uaugaccuug uaaagaggaa gaugaaaauc gcgggggauu ccgagaagaa acaaauuaag      180

aaaaauuucu ggggagugcg gcuguuugac gaaggccaga cggcugccga ucgccgaaug      240

gcacguaccg ccagaaggcg gaucgagcgc agaaggaacc ggauuucuua cuugcagggu      300

aucuucgcug aagagauguc aaagacagac gcaaacuuuu ucugccgcuu aagugauagc      360

uuuuaugucg acaaugaaaa gcgaaauucc agacauccgu ucuuugccac uauagaggag      420

gaaguggagu accacaaaaa cuaucccacc aucuaccauc ugagggagga gcuaguuaau      480

ucuucggaaa aggcugaucu ccggcuggug uaucuugccc uggcgcacau uaucaaauac      540

cgugggaacu ucuugauuga aggagcacuc gacacacaga auaccucagu cgauggcauc      600

uacaagcaau uuauacagac uuauaaccag guguucgcca gcggcauuga ggacgggagu      660

cugaaaaagc uggaagauaa uaaagacgua gcuaagaucc ucguugagaa ggugacacgc      720

aaagaaaagu uagagaggau ucugaaacuu uacccuggag agaaguccgc cgguauguuu      780

gcacaguuca ucucuuugau cgucggcagc aaagggaacu uucaaaagcc auucgaccug      840

auugaaaagu ccgauauaga gugugcuaaa gacucauaug aagaggaucu agaaagucuc      900

cuggcccuua ucggagacga guacgcggag cuguucgugg ccgcaaagaa ugcuuauagc      960

gccguggucu ugucuuccau uaucaccguu gcugaaacgg agacuaacgc caaacucuca     1020

gcaagcauga uagaaagguu ugauacccau gaggaagacc ugggcgaguu aaaggccuuc     1080

auuaaacugc accuccccaa gcacuacgag gaaaucuuua guaauacaga gaagcauggu     1140

uaugcugggu acaucgaugg aaaaacuaag caggcggacu ucuacaaaua uaugaagaug     1200

acccuugaaa acauugaggg cgcagauuac uuuaucgcca aaauugagaa ggaaaacuuc     1260

cugcggaagc agcgaacauu ugacaauggc gcuauaccuc accaguugca ucuagaggaa     1320

cuggaggcca uccuccacca acaggcaaaa uauuacccau uccugaagga aaacuaugau     1380

aaaauuaagu cucuugugac cuuucgcauc cccuacuucg uagggccucu ggccaaugga     1440

caguccgagu uugcuugguu gacgagaaaa gccgacggug agaucaggcc auggaacauu     1500

gaagagaagg uggacuucgg caaaagcgca gucgauuuua uagaaaagau gacuaauaag     1560

gacacauauc ucccgaaaga gaacgugcug cccaagcacu cguuaugcua ccaaaaauac     1620

cugguuuaua augaacuaac caaggugcgg uacaucaacg aucaggggaa aacuucuuau     1680

uucuccggac aggagaagga gcagauuuuc aaugaccucu uuaagcaaaa acgcaagguc     1740

aaaaagaaag aucuggaacu uuucuugaga aacaugucac auguggagag uccuacaauc     1800

gaaggccugg aggacagcuu uaauuccucu uacagcaccu aucacgaucu ccugaaggua     1860

gguauuaagc aggaaauccu ugacaaccca guuaauacgg agaugcugga gaacauagug     1920

aaaaucuuga ccgucuucga agauaagagg augauuaaag agcagcucca gcaauuuuca     1980

gacgugcugg auggggucgu guuaaagaaa cuggaacguc ggcauuacac aggcugggga     2040

cgacuaagug cgaagcuccu gaugggcauc agagacaagc agucccaccu uacuauuuug     2100

gauuaucuga ugaaugacga cgggcucaac aggaaccuga ugcaguuaau caaugauagc     2160

aaccugucuu ucaaaucaau aauugagaag gagcagguua ccacagcuga caaagauauc     2220

caaaguauug uggccgaccu ugcaggaagc cccgccauca agaaagguau auugcagucc     2280

cucaagaucg uagaugaacu ggucucugug augggcuacc cuccacagac uauuguugug     2340

gagauggcuc gcgaaaauca gaccacaggg aaaggaaaga acaauucgcg gcccagauac     2400

aagagccuag agaaagccau caaggaauuu ggcucccaaa uucugaaaga gcauccgacc     2460

gacaaccagg agcucaggaa uaaccgccug uaucuuuacu auuugcagaa ugguaaggau     2520

auguacacug ggcaagaccu ggauauccac aacuuaucaa auuaugacau agaucacauu     2580

gucccucaga guuucaucac ggacaacucu aucgauaauc ucgugcugac aagcuccgca     2640

ggcaaccgag aaaaaggaga cgacguaccc ccacuugaga uugugcggaa gcguaagguc     2700

uuuugggaaa aacuguacca gggcaauuug augucaaaga gaaaauucga uuaucucacc     2760

aaggcugaga gagggggacu gacugaagcc gacaaagcgc gcuuuaucca ucggcagcua     2820

guugagacca gacaaauuac aaagaacgug gccaacauac ugcaccagag guucaauuac     2880

gagaaggaug accaugguaa caccaugaaa caggugcgaa ucgucacucu caagagcgcu     2940

cugguuucuc aguuucgcaa acaauuccag cuuuacaagg ugcgggaugu gaaugacuau     3000

caccacgcac augaugccua cuugaacggc guagucgcua auacacuguu aaaaguguau     3060

ccucagcucg aacccgaguu cguuuacggg gacuaucacc aguuugauug guucaaggcc     3120

aacaaggcaa cggcgaaaaa gcaauuuuac accaauauua ugcuguucuu ugcccagaaa     3180

gacagaauca uugaugaaaa cggagagauc cuuugggaca agaaauaucu ggacacugug     3240

aagaagguca uguccuacag gcagaugaau auagugaaaa agacagaaau ccaaaaaggc     3300

gaguucagua aggcuaccau uaaaccaaag gguaacagcu cuaaacuaau cccccguaag     3360

acaaauuggg auccuaugaa guacgggggc cucgacuccc caaacauggc cuaugcaguu     3420

gugauugagu acgcuaaagg aaagaauaaa cugguauuug aaaagaaaau cauacgcguc     3480

acuauuaugg agcggaaggc cuucgaaaag gaugagaaag cauuuuugga ggaacagggc     3540

uauagacagc cgaaggugcu ggccaaauua cccaaguaca cccucuauga gugugaagag     3600

gggaggcgac ggaugcuggc uucagcgaac gaagcccaga aaggaaauca acaggugcuu     3660

ccuaaccauc uggucacguu gcuccaccau gcagcuaacu gcgagguuag ugacggcaag     3720

agccuggauu acaucgaguc gaaucgcgaa auguucgccg agcuacuggc ccacgugucc     3780

gaauuugcua agagauauac ccuugcagag gccaaccuca auaaaaucaa ccagcuguuc     3840

gaacagaaua aggaggguga cauuaaagcg auagcucaau cuuucguaga uuugauggcc     3900

uuuaacgcaa ugggggcccc agcuagcuuc aaguuuuucg agacaacuau cgaaaggaaa     3960

cgguacaaua accugaagga guuacucaau ucaaccauua ucuaccagag uauuacagga     4020

cuguaugaau cccgaaagag acuugacgau aaacgccccg cagccacuaa gaaagcgggc     4080

caggcuaaga aaaagaaaug a                                               4101


<210>  3482
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3482
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc aaagaaacca       60

uacagcauug gacuggacau agguaccaac uccguuggcu gggccguggu aacagaugac      120

uauaaggugc cggcgaagaa aaugaagguc cucgggaaua cugauaaauc ucauaucgag      180

aagaaccuuc ugggagcuuu guuauucgac ucaggcaaua cggccgaaga uaggcggcug      240

aaacgcaccg cacgacguag auacacaagg cggcgcaacc ggauucuaua ucuccaggag      300

aucuuuagug aagagauggg uaagguggac gauagcuucu uucacaggcu ggaagacucc      360

uuccuuguua cugaggauaa gagaggggag cgccauccca uuuuuggaaa ucuggaagag      420

gaagugaaau accacgagaa cuucccuacc aucuaucauu ugcgacaaua ccucgccgac      480

aauccagaga aggucgaucu gagacuggug uaucucgcuu uagcacacau aaucaaauuu      540

aggggccacu uccugauuga aggcaaguuu gacacacgga acaaugaugu acagcgucuu      600

uuccaggagu uuuuggccgu uuacgacaac accuucgaaa auucuucgcu gcaggagcaa      660

aacgugcagg ucgaagagau ccuaacugac aaaauuucaa agagcgcuaa aaaggaucgc      720

gugcucaagc uguuucccaa ugagaaaagu aacgggagau ucgccgaguu ccuuaagcug      780

aucgugggaa aucaggcgga cuuuaaaaag cauuucgagu uggaagagaa agcaccucuc      840

caguuuucca aggauacaua cgaagaggag cuggaagucu uacuggccca aauaggugac      900

aacuaugcug aacucuuccu uucugccaag aaacuguacg auagcauuuu gcuauccggc      960

auccugaccg uuacggacgu ggggacuaag gcaccacucu cagcuaguau gauucagagg     1020

uauaacgaac accagaugga ucuggcccag cuuaaacaau uuauccggca gaagcugagc     1080

gacaaauaca augagguauu cucugaugug uccaaggacg gauaugcggg cuacaucgau     1140

gguaagacca accaggaagc cuuuuauaaa uacuugaagg ggcuccugaa uaaaauugag     1200

ggaucaggcu acuucuuaga caagauagag cgagaagacu uucugcgcaa acaaagaaca     1260

uucgauaacg gcagcauccc ccaucagauu caccuacagg agaugagggc aaucauacgg     1320

cgccaggcug aauuuuaucc uuuccucgcc gacaaucaag auagaauuga gaagcugcuu     1380

acuuuuagga ucccauacua ugucgggccg uuggcucgug gaaagaguga cuucgccugg     1440

cugucucgga aauccgcaga uaagaucacc cccuggaacu ucgacgagau uguggauaaa     1500

gaaagcucgg ccgaggcuuu uaucaaucga augacaaacu acgaccucua ucugccuaau     1560

cagaagguuc uuccaaaaca cucucuguug uacgaaaagu ucaccgugua uaacgagcuc     1620

acgaaaguca aguacaagac ugaacagggu aaaacagcgu uuuucgaugc aaauaugaag     1680

caggagauuu uugacggcgu guucaaagua uacagaaagg uuaccaaaga uaagcugaug     1740

gacuuuuuag agaaggaguu cgaugaauuu aggauagugg accugacugg gcuagacaaa     1800

gaaaacaagg ucuucaaugc cuccuaugga acauaccaug aucucugcaa aauccuggac     1860

aaggauuuuc uugacaacag uaaaaaugag aagauuuugg aagauaucgu gcugacccuc     1920

acgcuguucg aggaccgcga gaugauccgg aagagauuag aaaacuauuc agaucugcuu     1980

accaaagagc aagucaagaa auuggaaagg cgccacuaca caggcugggg ucgacucagc     2040

gcugagcuga uucaugggau acggaacaag gaaucccgua aaacuauccu agacuaucug     2100

auugaugacg gcaauucuaa cagaaauuuu augcagcuca ucaacgauga cgcccugagc     2160

uucaaggagg agauugcaaa ggcccaggug aucggagaaa ccgauaaucu uaaccagguu     2220

gugucagaca uagcuggcag ucccgccauc aaaaagggga uuuugcaauc ccugaaaauc     2280

guagacgagu uagucaagau uaugggacac cagccugaaa auaucguggu ugagauggca     2340

agggagaacc aguucacaaa ucagggucgc cggaacagcc aacagagacu caaaggccug     2400

acugauucua uaaaggaauu ugggucacag auucuuaaag agcauccagu ggaaaauagu     2460

caacugcaga acgacagguu guuccucuac uaucugcaga auggacgaga uauguacacc     2520

ggcgaggaac uagacaucga uuaccugagc caguaugaca uugaucacau cauaccccaa     2580

gcguuuauca aggacaacuc cauugauaau cgcguccuca caucuucgaa ggagaaccgg     2640

gguaaaagcg acgaugugcc guccaaggac guagugagaa aaaugaaguc auacuggagu     2700

aaacugcuuu cugcuaaguu gaucacccag aggaaguucg acaaccugac uaaagccgag     2760

cgugggggcu uaacggauga cgauaaggca ggauuuauua aacgccagcu cguugaaaca     2820

cggcagauca ccaagcacgu cgccagaaua cuggacgaga gguucaauac ugaaaccgau     2880

gagaacaaua aaaagauucg acaagugaag aucgugacac uuaaaagcaa ccuggucucc     2940

aauuuucgga aggaguucga acuauauaaa guucgcgaga ucaacgacua ccaucacgcu     3000

caugaugccu aucucaaugc agugauuggc aaggcucugu ugggggugua cccucagcug     3060

gaacccgagu uuguauaugg agacuaccca cacuuccacg gucauaaaga aaacaaggcc     3120

accgcgaaga aauuuuucua cucaaauauc augaacuucu uuaagaaaga ugacgucaga     3180

acugauaaga auggcgagau uauauggaaa aaggacgaac acaucagcaa cauuaagaaa     3240

guguuaucuu auccucaggu uaauaucgug aagaaagucg aggagcagac agggggauuc     3300

uccaaggaaa guauucuccc caaaggcaac agcgauaagc ugaucccaag gaaaacgaag     3360

aaguuuuacu gggacaccaa aaaguauggu ggguucgacu cucccauagu ggccuacucc     3420

auccuuguua uugcugauau cgagaaaggc aagaguaaaa agcugaagac ugugaaagca     3480

uugguaggag ucacaauuau ggaaaagaug accuuugagc gggacccugu ggccuuccuc     3540

gagcgaaaag gcuauagaaa ugugcaagaa gagaacauca uaaagcugcc aaaauacuca     3600

cuauuuaagc uggaaaacgg gcgcaagagg cuucucgcua gcgcccguga gcugcagaaa     3660

ggaaaugaaa uugucuugcc gaaccaucug ggcacauuac ucuaucacgc aaagaauauc     3720

cauaaaguug augagcccaa gcaccuggac uacguggaua aacacaagga cgaguucaag     3780

gaacuucugg auguaguguc gaacuuuucc aaaaaguaca cuuuggcgga ggguaaucuc     3840

gagaaaauca aggaacugua ugcccagaac aauggggaag accuaaaaga gcuggcuucu     3900

agcuucauua accuucucac cuuuacggcc auaggagcuc cugcaaccuu caaguuuuuc     3960

gauaaaaaua ucgaccggaa gagauacaca ucaacuaccg agauucugaa cgccacauug     4020

auccaucaaa guauuacugg cuuauaugaa accaggaucg aucugaauaa gcucgguggc     4080

gacaaacgcc cagcagccac gaagaaagcu gggcaggcga agaaaaagaa guga           4134


<210>  3483
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3483
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc acagacaaca       60

aaccugagcu acauucucgg acuugaccug gguauagccu ccguuggcug ggcgguggua      120

gagaucaaug aaaacgagga uccaauuggg uugaucgacg ugggagucag gauuuucgaa      180

cgggcugagg ugccgaagac uggcgaaucu uuagcccugu cacgccgacu agcacguagu      240

acgagaaggc ucauccggcg cagagcccau aggcugcuuc uggcuaaacg guuuuugaag      300

cgcgagggua uacucagcac caucgaucug gagaaggggc ugcccaauca agcaugggaa      360

cuccgaguug ccggauuaga gagaaggcug uccgcuauug aauggggcgc cgugcuuuug      420

caccugauca aacaucgggg cuaucuaucu aagcguaaaa acgagucgca gacaaauaac      480

aaggagcucg gggcgcugcu uucaggaguc gcacagaauc accagcuguu gcaaagcgac      540

gauuaccgca cuccugccga acucgcucug aaaaaguucg ccaaggagga aggucauauu      600

agaaaccaga ggggcgcaua uacccacaca uuuaaucggu uagaccugcu cgcugagcuu      660

aaccuguugu ucgcccagca gcaccaauuu gggaauccac auugcaaaga acacauccag      720

caguacauga ccgagcuacu gauguggcag aagcccgcgc ucaguggaga ggccauacug      780

aaaaugcuug gcaaguguac ucaugagaaa aacgaguuca aggcagcuaa acacacauau      840

uccgccgaac gauuugugug gcugaccaag uugaauaacc uccgcauucu ggaggauggu      900

gcugaaagag ccuuaaauga ggaggaaagg caacugcuaa ucaaccaccc uuacgagaag      960

ucuaaacuca cguaugcaca gguacggaag cugcuugggu ugagcgaaca ggccauuuuc     1020

aaacaucugc gcuacuccaa ggagaacgcu gaaucagcga cuuuuaugga gcucaaagca     1080

uggcacgcca ucagaaaggc ucuggagaau caaggacuua aggacaccug gcaggaucug     1140

gccaaaaagc cagacuugcu cgaugaaauc ggcacagcau ucagucugua caaaacugac     1200

gaggauauuc agcaguauuu aaccaacaag guucccaaua gcgugauaaa cgcccugcua     1260

gucucucuca auuuugacaa auucaucgaa cugucccuua agucauugag gaagauucug     1320

ccucucaugg agcaaggcaa acguuacgac caggcuugcc gggagaucua ugggcaucac     1380

uacggagaag ccaaccagaa gacaagccag cuguuaccag caauaccggc gcaagagauu     1440

cgaaaucccg uggugcugag aacccuuagu caggcuagga aagucaucaa cgccaucauu     1500

cgccaguaug guucuccugc acggguucau aucgaaacgg gcagagaguu ggggaagucc     1560

uuuaaagaaa ggcgcgagau ucagaagcaa caggaggaua aucgaacuaa gcgggaaagc     1620

gccgugcaga aauucaagga acucuucucg gacuuuucuu ccgaaccaaa aucaaaggau     1680

auacugaaau uccgucuaua cgagcagcaa cacggaaagu gucuguauag uggcaaagaa     1740

aucaacauuc acagacucaa ugagaagggu uacguagaga ucgaccaugc ucugcccuuu     1800

agcaggacau gggaugacuc cuucaacaau aaggugcuug ucuuggccuc ugaaaaccag     1860

aauaaaggga accagacccc uuacgagugg cugcaaggca agaucaacag cgaacgcugg     1920

aaaaauuuug uggcauuagu ucucggauca cagugcagug cugccaagaa acagcggcug     1980

cuuacucagg ugauugauga caacaaguuc auagauagaa aucugaacga cacaagguau     2040

aucgcgcgau uuuuguccaa uuacauucaa gagaaccucc ugcuagucgg caagaauaaa     2100

aagaacgugu ucaccccaaa ugggcagauc acggcccugc uccgcagccg guggggacug     2160

auuaaagcua gagaaaacaa uaacaggcac caugcacuug augccaucgu aguugcuugu     2220

gccacccccu cuaugcagca gaagauaaca cguuuuaucc gcuucaaaga ggugcacccg     2280

uauaagauug agaaucggua cgaaaugguc gaccaagagu caggugaaau cauuaguccu     2340

cacuuucccg agccaugggc auauuucaga caggagguga acaucagggu cuuugacaau     2400

cauccugaua cuguguugaa ggaaaugcug cccgaccgac cacaggcgaa ccaccaguuc     2460

guucaacccu uauucgugag ccgggccccu acccgcaaaa uguccggcca ggggcauaug     2520

gagacaauaa agucugcuaa aagacucgcc gaaggaauuu cgguacugag gaucccacuu     2580

acucagcuga agccgaaccu acucgagaau auggucaaca aagaacggga gcccgcacug     2640

uacgcuggcu ugaaggcccg acuggcagag uuuaaucaag auccugccaa ggcuuucgcg     2700

accccauuuu auaaacaggg ugggcaacag gugaaggcca uuagaguuga acaggugcag     2760

aaaagcggcg ucuuagugcg cgagaacaau ggaguagcag acaacgcuuc caucgugagg     2820

acagauguuu ucauaaagaa uaacaaauuu uuccucgucc ccaucuacac cuggcaagug     2880

gccaagggca uucugccuaa uaaagccauc guggcucaca agaacgaaga cgagugggaa     2940

gagauggaug agggggcaaa guuuaaauuc ucacuuuuuc caaaugaccu ggucgaauug     3000

aagacuaaaa aggaguacuu cuuuggauau uacauugguc ucgaucgugc cacgggcaac     3060

aucagucuga aagaacauga cggggagaua ucuaagggaa aggauggcgu uuaucgggug     3120

ggugugaaac uagcgcugag cuucgaaaag uaccagguag acgagcuugg gaaaaauaga     3180

cagauuugca ggccccagca gcgccagccg guccgaaaga gaccugcugc caccaaaaag     3240

gcaggccaag ccaagaaaaa gaaauga                                         3267


<210>  3484
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3484
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc agccgcguuc       60

aagccaaacc cgauuaauua cauacuggga cucgacaucg guauugcuag cguuggcugg      120

gccauggugg agaucgauga agacgagaac cccauuugcc uuaucgaucu ggggguaagg      180

guguuugaac gggcagaggu cccuaaaacc ggagacuccu uggccauggc ucgccgauua      240

gcacguucug ugagaaggcu gacacggcgc agagcccaua ggcuacuccg ggcucgccga      300

cugcuuaaga gagaaggcgu ucugcaggcc gcggauuucg acgagaaugg uuugauaaag      360

ucacucccaa acacucccug gcaacugagg gcagccgcuc uggaucggaa acucacgccu      420

uuagagugga gugccgugcu gcuucacuug aucaagcauc guggguaucu gagccagcgc      480

aaaaaugaag gagagaccgc agacaaggaa cuaggcgcuc uccugaaagg cgucgccgau      540

aacgcgcacg cccuucagac aggggacuuu agaacuccag cagagcuggc uuugaauaag      600

uucgagaagg aauccggaca uauuaggaac cagcggggug auuacucuca caccuuuucg      660

cgaaaagacc uccaagccga gcugaucuua cuguucgaaa agcagaaaga guuuggcaau      720

ccccacgugu cagggggacu caaggaaggc auugagacac uucugaugac ccagcgcccu      780

gcuuugagcg gugaugccgu acagaaaaug cuagggcauu guacuuucga gccagcagaa      840

ccgaaggccg cuaaaaacac auauaccgcg gagagauuua ucuggcugac gaagcucaau      900

aaccugagga uacuugaaca aggaagugag cggccccuga cugacaccga acgcgcaaca      960

uugauggacg agccuuacag aaaguccaaa cucacuuaug cccaggcuag gaagcuguua     1020

ggccuggagg auaccgccuu cuuuaaaggc cuacguuacg ggaaggacaa ugcagaagcc     1080

ucuacacuca uggagaugaa ggcuuaucac gccauuagcc gggcacugga aaaggaggga     1140

cuuaaggaua aaaagucccc auugaaccug ucacccgaac uccaggacga gaucgguacc     1200

gcguucaguc uguuuaaaac ggaugaggac auuacuggcc gacuuaagga uagaauccag     1260

ccugaaaucc uggaggcuuu gcucaaacau auuagcuucg acaaguucgu ucaaauaucu     1320

cugaaggccu uaaggcgcau cgugccacug auggaacagg ggaaacggua cgaugaggca     1380

ugcgccgaga uuuacggaga ccacuauggc aagaaaaaua cagaagagaa gaucuaccua     1440

cccccgauac cugcugauga aauuagaaac cccgucgugc ucagggcccu gucccaggca     1500

cgcaaaguga ucaacggugu cguucgacgg uaugggucac cagcucguau ccacauugag     1560

accgccagag aagugggcaa gagcuuuaag gacaggaaag agaucgagaa gcgccaagaa     1620

gagaaucgga aagacagaga aaaggcggcc gcuaaauuca gggaguacuu uccuaacuuc     1680

guaggagaac ccaagaguaa agauauucuu aaguugcgac uguaugagca gcagcauggc     1740

aagugucucu acucugggaa agagauaaau cugggacgcu uaaacgaaaa ggguuaugug     1800

gagaucgacc acgcacugcc auuuucccgg acuugggaug acagcuucaa uaacaaaguc     1860

cuuguguugg gcucggaaaa ucagaacaag gggaaucaaa cacccuacga guacuuuaac     1920

ggaaaagaua auucuagaga auggcaggag uucaaggcca ggguugagac cucccguuuu     1980

ccucgcucaa agaaacagcg gauucuccug cagaaguucg acgaagaugg cuuuaaagag     2040

agaaaccuaa augacacgag guaugugaac cgauuccugu gccaauuugu cgcugaucgg     2100

augcgccuca ccgguaaggg gaaaaagaga guguucgcca guaauggcca gaucacaaac     2160

cugcuuaggg gauucugggg cuugcggaag guacgagcag aaaacgacag acaucacgcg     2220

cuggaugccg uuguggucgc uuguagcacu guggccaugc agcagaaaau cacccgcuuu     2280

gucagguaca aggagaugaa ugcauucgac gggaaaacaa uugauaagga gacuggagaa     2340

guguuacauc agaaaaccca cuuuccacag ccgugggagu ucuuugcuca ggaaguuaug     2400

auacgugugu ucgguaagcc cgacggcaag ccugaguuug aagaggccga cacaccagag     2460

aaacuccgga cccugcuugc agaaaagcug uccucuagac ccgaggccgu acacgaauau     2520

gucacuccuu uguucgugag cagggcucca aaccgcaaaa ugucagggca gggacauaug     2580

gagacgguua agagugcgaa acgacucgau gaaggcgugu ccguccugag agugccccua     2640

acacaacuga agcucaaaga ccuggagaag augguaaauc gggagaggga accgaaacuu     2700

uacgaggccu ugaaagcacg ccuggaagcu cacaaggaug acccugccaa agccuuugcu     2760

gagccauucu auaaguacga uaaagcaggu aaccguaccc aacaggugaa ggccguuaga     2820

gucgaacaag ugcagaagac uggggugugg guccggaauc auaacggcau cgcggacaau     2880

gcuaccaugg uuagggugga uguguuugag aaaggagaca aguauuacuu aguacccauu     2940

uacagcuggc aggucgccaa aggcauccuc ccugaucgcg cagugguuca ggggaaggac     3000

gaggaagauu ggcaacugau ugacgauucu uucaacuuua aauucucacu ucaccccaau     3060

gaccuggugg aggucaucac aaagaaggcc cgaauguucg gauauuuugc uaguugccac     3120

agaggcaccg guaacauaaa uauccggauu caugaccuag aucacaaaau cgggaagaac     3180

ggcauucucg aaggaaucgg ugugaaaacu gcacugagcu uccagaagua ccagauagac     3240

gaguugggca aagagauuag gccaugucgc cugaagaaga gaccucccgu uaggaaacgg     3300

ccagccgcga caaagaaagc ugggcaggcc aagaaaaaga aguga                     3345


<210>  3485
<211>  3351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3485
auggcaccca agaaaaagag aaaagugggc auccacgggg ucccugcugc aaaguaccac       60

guuggaauug acguggguac cuucagcgua ggccuggccg cgauagaggu ggaugacgcu      120

gggaugccaa ucaaaacacu cucccuuguc ucucacauuc augauucagg acuggacccg      180

gaugaaauca agagugccgu gacuagguug gcaagcuccg gcauugcccg gcgcacgcga      240

cguuuauaua gaaggaagcg gcgcagacug cagcaacuag acaaauuuau ccagaggcag      300

gguuggcccg uuauagagcu cgaagauuac ucugacccuc uguauccaug gaaggugcgg      360

gcugagcuug cagccucgua caucgcugau gaaaaagagc gcggggagaa gcugucaguc      420

gccuugcgac acauugcgag acauagggga uggcggaacc ccuaugcaaa agugagcagu      480

cucuaccugc cugacggccc auccgaugcc uucaaggcua uccgugaaga gauuaagcgc      540

gccucuggcc agcccguacc ugaaaccgca acaguugggc aaauggugac ucugugcgag      600

cucggaaccu uaaaacugag aggugagggc gggguccuua gcgcuagguu gcagcagucc      660

gacuaugccc gggaaaucca ggagauaugu cgaaugcaag aaauuggaca ggagcuguac      720

cgcaagauca uugauguggu guuugcggcc gaaucaccaa aaggcagugc aagcucuaga      780

gucgguaagg acccgcuaca gcccgggaaa aauagggcuc ucaaggccuc cgacgcuuuc      840

cagcgguacc gcaucgccgc acugaucgga aaccuuagag uuagggugga uggcgagaaa      900

cguauucugu caguagagga aaagaauuug guguuugacc accucgucaa ccugacaccu      960

aagaaagagc cagaaugggu gaccauagcc gagaucuuag gcauugaucg ggggcaacug     1020

aucggaacug cuacaaugac cgacgauggu gaacgagcgg gcgcaagacc cccuacgcac     1080

gacacuaaua ggagcauagu uaacagucgc auugccccac ucguggauug guggaagacc     1140

gcuucugccc uugagcagca ugcaaugguc aaagcccugu ccaaugcuga gguggacgau     1200

uucgacagcc ccgaaggggc caagguacag gcauuuuucg cggauuugga cgacgauguu     1260

cacgcuaaac uagacucgcu gcaucucccg gugggacggg ccgcauauuc ugaggauaca     1320

cuggucagac uuacuaggcg caugcugucc gacggcgugg auuuguacac cgcccgacuc     1380

caagaauuug guaucgagcc uucauggaca cccccaaccc cucggaucgg ggaacccguc     1440

ggcaacccag cuguggaccg uguucugaag acggugagua gaugguuaga gagcgccacu     1500

aagacauggg gagcacccga gaggguaauu aucgaacacg uccgcgaggg cuucgugacc     1560

gaaaaacggg cuagagagau ggauggggac augaggcgac gcgccgcgcg gaaugccaag     1620

cuguuucagg agaugcagga aaaacuaaac guucagggaa agccuuccag agcugaucuc     1680

uggagguauc aaucugugca gcgucagaau ugccagugug cauacugcgg uagcccaauu     1740

acuuucucaa acagugagau ggaccacaua gucccgcgcg ccggccaagg guccacaaau     1800

acccgggaaa accugguggc uguaugucau agaugcaauc agagcaaagg aaacacgccc     1860

uuugccaucu gggcaaagaa caccucuauu gagggcgugu caguuaagga agcggucgag     1920

aggacacgac acugggugac ugauaccggu augcggagua cagacuucaa aaaguucacu     1980

aaagccgugg ucgagcgcuu ucagagagcu accauggaug aagagaucga cgccaggagc     2040

auggaauccg uugcauggau ggcuaaugag cuucggucuc gaguggccca gcauuucgca     2100

ucgcacggga caaccgugag aguauaucgc ggcagcuuga cugccgaagc uaggcgugcg     2160

uccggaaucu caggcaagcu gaaauuuuuc gacggggucg gaaagagucg gcucgauaga     2220

aggcaucacg ccauugacgc agcugugaua gccuuuacgu cugauuacgu ugccgagaca     2280

cuggcugugc gcagcaaccu uaagcaaucc caggcacacc gacaggaggc cccucagugg     2340

agagaguuca ccgguaaaga cgcggaacau cgggcugccu ggagggucug gugucaaaag     2400

auggaaaaac ugucagcauu gcucacugag gaucugcgcg acgaucgugu gguugugaug     2460

agcaauguaa gauuacggcu gggcaacggg ucugcccaca aggaaaccau cggaaaacua     2520

uccaagguca aacucaguag ccagcugucu guguccgaca uugauaaggc uucaagugag     2580

gcacuuuggu gcgccuugac aagggagcca ggcuuugacc ccaaggaagg ucugccugcg     2640

aauccagagc gccauauccg agugaacggg acccacgucu augcuggcga uaauauugga     2700

cucuuccccg uuagcgccgg cucgaucgca cugagagggg gauacgccga auuaggcucc     2760

ucuuuucacc augcucgggu guacaaaaua acuagcggua agaaaccggc cuucgcaaug     2820

cugaggguau auacaaucga ccuuuugccu uaccgcaacc aggaucucuu uucaguggag     2880

cugaagccac aaacgaugag uaugagacag gcugagaaaa agcuaaggga cgcccuggcg     2940

accgggaaug ccgaauaucu cggauggcug gucguggacg augagcuugu uguggacacu     3000

uccaagauug caacagauca ggucaaagcu guggaagccg aguugggcac cauccggcgu     3060

uggcgcguug acgguuucuu uucucccagc aagcugcgau uaagaccucu ccagaugucc     3120

aaagaaggca uuaagaaaga gucagcuccc gagcugagca agaucauaga uaggccaggg     3180

uggcuuccug cagugaacaa gcuguucagu gacggaaaug ucacaguagu gcggcgcgau     3240

ucuuugggca gaguuaggcu cgaauccacu gcccaucugc ccgugaccug gaaaguccaa     3300

aagcggccag ccgcuacgaa aaaggcgggg caggccaaaa agaagaaaug a              3351


<210>  3486
<211>  3051
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3486
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc agccaggauu       60

cuggcguucg acauaggaau cagcuccauu gguugggcuu uuucugagaa cgaugaacuc      120

aaggacugcg gcguucggau cuucaccaaa guggagaauc caaagacagg ggaaucacuu      180

gcccugccgc gccgauuggc acguagugcc agaaagaggu uagcucggcg caaagcaaga      240

cugaaccauc uaaagcaccu cauugccaau gaguuuaaac ugaacuacga agauuaucag      300

agcuucgacg agucccuugc uaaggccuac aaaggaucuc ugaucucgcc cuaugaguug      360

agguuucggg cgcucaauga acugcuguca aagcaagauu ucgcacgcgu aauacuccau      420

aucgccaagc gaagaggcua cgacgauauu aaaaacagcg acgauaagga gaaaggugcu      480

aucuuaaagg ccauuaaaca gaaugaagag aagcuggcaa acuaucagag ugugggggag      540

uaccuuuaua aagaauacuu ucagaaguuc aaggaaaauu ccaaagaauu uacuaacguc      600

aggaauaaga aagagucuua cgaacggugu aucgcucaga gcuucuugaa ggacgagcug      660

aaacuaauau uuaagaagca acgugaguuc ggauuuuccu ucucuaaaaa guucgaagag      720

gaagugcuca guguugccuu uuauaaacgc gcgcugaagg auuucagcca ccuugugggc      780

aacugcucau uuuucacgga cgagaaaaga gccccuaaaa auuccccacu ggcauuuaug      840

uucgucgcuu ugaccaggau uaucaaccuc cugaauaacu uaaagaacac agaaggcauu      900

cuguacacua aagaugaccu caaugcccuu cugaacgagg uguugaagaa ugggacccua      960

acauauaaac agaccaagaa acugcucgga cugucagacg auuacgaguu uaagggugaa     1020

aagggcacuu auuucaucga guuuaaaaag uacaaagagu ucaucaaggc ucuuggggag     1080

cauaaccuga gccaggacga uuugaaugaa auugccaaag acauaacacu caucaaggau     1140

gagauuaaac ugaagaaggc auuagccaaa uaugaccuga accaaaauca gaucgauagu     1200

cuaucuaagc ucgaguuuaa agaccaccug aacauauccu ucaaggcucu uaaauuggua     1260

acuccccuga ugcucgaagg aaagaaguac gaugaggcgu guaaugaacu gaaccuuaaa     1320

guugcaauua augaggacaa gaaagauuuu cugccugccu ucaacgagac guacuauaag     1380

gacgaaguga cuaauccagu cguguugcgg gcuaucaaag aguaccgaaa ggugcucaac     1440

gcccuguuaa agaaauaugg caagguccac aaaaucaaua uugaacuggc acgcgagguu     1500

gguaagaacc auagccagag agccaaaauc gaaaaggagc agaacgagaa uuacaaggcu     1560

aaaaaggacg ccgaacuaga gugcgaaaaa cucgggcuga agauuaacuc gaaaaauaua     1620

cuuaaguuga ggcuguucaa agagcaaaag gaauuuugug cauauucugg agagaagauc     1680

aaaauuuccg aucuccagga cgagaagaug cuggaaaucg aucacaucua ccccuauuca     1740

cggaguuucg acgauagcua caugaacaaa guguuaguau uuaccaagca gaaucaagag     1800

aaacugaacc agacaccuuu cgaagcguuu ggcaaugacu ccgcuaagug gcagaagauu     1860

gaggugcuug ccaaaaacuu gccaacuaag aaacagaagc gcauacucga uaaaaauuac     1920

aaggacaagg aacaaaagaa cuucaaggau agaaaucuga acgacaccag guauaucgca     1980

cgucuagucc ugaauuacac aaaagauuau cucgacuuuc ugccgcuuuc ugaugacgag     2040

aacaccaagu ugaaugacac gcagaaaggc agcaaggugc auguugaggc caagucaggg     2100

augcugacca gugcuuuacg gcacacaugg ggauucuccg ccaaagaucg aaacaaccac     2160

cuccaucacg caauugacgc ugugaucauu gccuacgcga auaacagcau cgucaaggcc     2220

uuuucugauu ucaaaaagga acaggaguca aauagugcug aacuguaugc caaaaagaua     2280

agcgagcuug acuacaagaa caaaagaaag uuuuucgagc ccuuuuccgg uuucaggcag     2340

aaagugcugg auaagaucga cgaaauuuuc guaucuaaac cugagcgcaa gaaaccaucg     2400

ggcgcauugc augaagagac cuuucggaag gaagaggagu ucuaucaaag cuacggggga     2460

aaggaaggcg uucucaaagc ucuggagcua gguaagauca gaaaagugaa ugggaagauu     2520

gucaaaaacg gcgauauguu uaggguggac aucuucaagc acaagaaaac uaauaaguuu     2580

uacgccgucc ccauauauac aauggauuuc gcacugaaag ugcucccuaa caaggcgguu     2640

gcccgcucca aaaagggaga aauuaaggac uggauccuga uggaugagaa uuacgaauuu     2700

ugcuucucac uuuauaaaga caguuugauu cugauccaga ccaaggauau gcaggagcca     2760

gaguuugugu acuauaacgc uuucacgucu agcaccguau ccuuaauagu cucaaaacau     2820

gacaauaagu uugaaacacu cagcaaaaac cagaagaucc uguucaagaa ugccaacgag     2880

aaagaaguga uugcaaaguc uaucggcauu caaaaucuua aaguuuucga gaaguacauc     2940

guguccgcuc ugggggaagu cacuaaagcc gaguuucgac agcgggagga cuucaagaaa     3000

aagcgucccg cagccaccaa gaaagcugga caggcgaaga aaaagaaaug a              3051


<210>  3487
<211>  3462
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3487
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc aagcgaccug       60

guucucggac uugauauugg uauaggcucc guggggguag gaauccugaa caaggugacc      120

ggcgagauua uccauaaaaa uucuaggauu uucccagccg cgcaggcuga aaacaauuug      180

guccggcgca caaaccgaca aggucguaga uuagccaggc ggaagaagca ccgcagagug      240

aggcugaauc ggcuauuuga ggaaucaggg cucaucacug acuucacgaa aauaaguauc      300

aaccugaauc cguaccagcu ucgcguuaag ggacugaccg augaguugag caacgaagaa      360

cucuuuauug cacugaaaaa uauggugaag caucgaggca ucuccuaucu ggacgaugcc      420

ucugacgaug gcaacucguc agucggggac uacgcucaga uugugaaaga gaauagcaag      480

cagcucgaaa caaagacucc cggacaaauc caguuagaga gauaucagac cuacggucag      540

cugaggggcg auuucacagu agaaaaagac gggaagaaac accggcuuau aaacguuuuu      600

ccuaccagug cauaucguuc cgaggccuug cgcauucugc aaacucagca ggaguucaau      660

ccacagauca cagaugaauu uauuaacaga uaccuagaga uccucaccgg aaagaggaaa      720

uauuaccaug gccccgguaa ugaaaagucu cggacggacu acgggcgaua ucgcacuagc      780

ggagagaccc uggauaacau cuucggcauu cuuauaggca aaugcacauu uuacccugac      840

gaguucagag cugccaaggc guccuauacu gcacaagagu uuaaccuguu gaaugaccuc      900

aacaaucuga ccgugccaac agagaccaag aaauuaucaa aggaacagaa aaaccagauc      960

auuaauuacg ucaagaacga gaaagccaug gggcccgcua agcuguucaa guauaucgcc     1020

aaacuccuua guugugaugu ggcagacaua aagggauaca ggauugauaa aagcgguaag     1080

gcugaaaucc acacguuuga ggccuaucgg aaaaugaaga cucuggaaac auuggacauc     1140

gagcaaaugg aucgcgagac ccuagacaag cuggcguacg ugcucacucu gaauacagaa     1200

agagagggca uucaggaagc ccuugagcac gaguucgcag augggucuuu cucccagaaa     1260

caggucgacg agcugguuca auuuaggaag gcuaacucaa gcaucuucgg aaaaggcugg     1320

cauaauuuua gugugaaguu gaugauggag cucauuccug aacuguacga gaccucugaa     1380

gaacagauga cgauauuaac ccgucugggu aaacagaaga caacuuccag cucgaacaag     1440

accaaauaua ucgaugagaa gcuacucaca gaagagauuu acaauccagu aguggccaaa     1500

ucuguccggc aggcuaucaa gaucgugaac gccgcaauua aagaauaugg ggacuucgau     1560

aauauaguua ucgagauggc ccgagaaacu aacgaggacg acgagaagaa agcuauucaa     1620

aagauccaga aggcgaauaa agaugaaaag gacgcagcca ugcugaaagc ugccaaccag     1680

uacaacggca aggcagagcu uccgcacucc guguuucaug gacacaaaca guuggccacc     1740

aagauuagac uguggcacca acagggcgaa aggugccucu auacagggaa gaccaucuca     1800

auacaugauc ugaucaauaa caguaaucag uucgaggucg accacauucu uccccugagc     1860

aucacuuuug augacuccuu ggcuaacaaa gugcucguau acgccacggc aaaucaggaa     1920

aagggacaac gcacaccuua ucaggcgcug gauucuaugg acgaugcuug gagcuuccgg     1980

gaguuaaaag ccuuuguuag agagucaaag acccugagua acaaaaagaa ggaauaccua     2040

cucacugagg aagacauuuc caaauucgau gugaggaaga aauuuaucga gcgcaaucug     2100

gucgacaccc gauacgcaag ccgggugguc cuuaacgccu ugcaggaaca uuuccgugcu     2160

cacaagauag auacaaaagu gucuguugug agaggucaau uuaccucaca gcugaggcgc     2220

cauuggggca uugagaagac ucgggacaca uaucaccacc augccguaga cgcacucauc     2280

auugcugcca guagccagcu gaauuuaugg aagaaacaga agaacacgcu ggucuccuac     2340

ucugaggauc aacuuuugga caucgaaacc ggggagcuca uaucggauga cgaauauaaa     2400

gagagcgugu ucaaggcgcc auaccagcac uucguugaua cucugaaauc caaggaguuu     2460

gaagacucaa uccuauucag uuaucaggug gauucuaaau uuaauagaaa gauuagcgac     2520

gccacaaucu acgcuaccag gcaggcaaag gucggaaaag auaaggccga cgagacauau     2580

gugcugggca aaauuaagga uaucuacacu caagacgguu acgaugcuuu caugaaaaua     2640

uauaagaagg acaaauccaa guuucucaug uaccgacaug acccccagac cuucgaaaaa     2700

guaauugagc cuauccugga aaacuaucca aauaagcaga ucaacgagaa agggaaggag     2760

gugcccugua auccguuucu uaaguacaaa gaggaacacg gcuauauucg caaguacagc     2820

aaaaagggaa acggcccuga aaucaaauca uugaaguauu acgauucuaa gcuggggaac     2880

cacauugaca uaacgcccaa agauuccaau aacaagguug ucuuacagag ugugagccca     2940

uggcgggccg acguguacuu caauaaaacc acaggaaagu augagauccu cggucugaaa     3000

uacgcagauc uucaauuuga aaagggcacu gggaccuaua agauuucuca ggagaaauac     3060

aacgacauca agaaaaagga gggagucgau uccgacucag aguucaaauu uacacuguau     3120

aagaaugauu ugcuccuggu uaaagacacu gaaaccaagg aacagcaacu auucagauuu     3180

cugaguagga cgaugccuaa gcagaaacau uacguggagc ucaagcccua ugauaaacag     3240

aaguucgaag gcggugaggc gcugauuaaa gugcuuggga acguagccaa uagcggccag     3300

ugcaagaagg gauugggcaa aucgaacauc uccauauaca agguccguac agacgugcug     3360

gggaaucaac acaucauuaa aaacgaggga gacaagccaa aauuagauuu caagcgcccc     3420

gcugccacca agaaagcagg ccaggcuaag aaaaagaaau ga                        3462


<210>  3488
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3488
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc aacccaguuc       60

gagggauuua caaaccugua ccaaguuagc aagacucuca gguucgaacu uauuccacag      120

gguaaaacgc ugaagcauau acaggagcag ggcuuuaucg aagaggacaa ggcccggaau      180

gaucacuaua aagaauugaa gccgauuauc gaccgcauuu acaaaaccua ugcggaucaa      240

ugcuuacagc uggugcagcu agacugggag aaccucuccg cugccaucga uucuuaccga      300

aaggagaaaa cagaagagac ucguaaugca cugauagagg aacaggccac cuauagaaac      360

gcuauccaug acuacuucau ugggaggaca gauaaucuua ccgacgcaau caacaagcgg      420

cacgccgaga uuuauaaggg acuguuuaaa gcugaauugu ucaauggcaa gguacucaaa      480

caacugggua cugugacaac cacggagcau gaaaacgccc ugcuccgcuc auuugauaag      540

uucacuaccu acuuuagugg guucuacgag aauaggaaaa acgucuuuag cgcggaagac      600

aucuccacag caauacccca cagaauugug caggauaauu ucccuaaguu uaaagagaac      660

ugucacaucu ucacucgguu aauuaccgcc guuccaucuc ugcgcgagca uuucgaaaau      720

gugaagaagg cuaucggaau cuuugucucg acaucaauug aggaaguguu cagcuuuccc      780

uucuauaacc agcuuuugac ccagacgcaa auagaccugu acaaucagcu acucggcggc      840

aucagucgag aggccgggac ugagaaaauu aagggacuga acgaaguacu uaaccuggca      900

auccagaaaa augaugagac agcucacaua auugccuccu ugccucauag auuuauccca      960

cucuucaagc aaauccuguc ugacaggaac acccuuagcu uuauucugga agaguucaaa     1020

uccgacgaag agguuaucca gucauuuugc aaguauaaga cucuccuucg gaaugaaaac     1080

gugcuggaga cagcggaggc cuuguucaau gaacuaaaca guauugaucu gacccacaua     1140

uuuaucagcc acaaaaagcu cgagacgauu ucuuccgcac ugugugacca uugggauacc     1200

cuucguaaug cucuguacga acgcagaauc ucagaguuga cagguaaaau cacuaagagc     1260

gccaaagaga agguccagag gagucucaag cacgaagaca uuaaccugca ggagauaauc     1320

ucugcugccg gcaaagaauu auccgaggca uucaagcaga aaaccagcga aauucugucg     1380

caugcccacg cugcgcuaga ucaaccccuc ccuacaacuc ugaagaaaca ggaggagaag     1440

gaaauccuua agucucaguu ggacucccug cucgggcugu aucaucuucu ggauugguuu     1500

gcaguggacg agucaaauga aguggaucca gaguucagug cccgguugac cggaauuaaa     1560

cucgaaaugg agccgagccu guccuucuac aacaaggcuc gaaauuaugc cacuaaaaag     1620

cccuacucug ucgagaaauu uaaguuaaac uuccaaaugc cuacccuggc aagcggcugg     1680

gacguuaaua aagaaaagaa caauggugcc auccuauuug ugaagaacgg gcucuacuau     1740

cugggaauaa ugccaaaaca gaagggccgc uacaaagcuc uuucauucga gcccacugaa     1800

aagacgagug agggcuuuga uaaaauguau uacgacuauu ucccugacgc cgcaaagaug     1860

aucccaaagu gcuccacaca guugaaagcg guaaccgcuc acuuucagac ucacaccaca     1920

cccauucugc ucagcaacaa uuucaucgaa ccgcuggaga uuaccaagga gaucuacgau     1980

uuaaacaauc cugaaaaaga gcccaagaaa uuucaaacug ccuaugcaaa gaagacaggg     2040

gaccagaaag gauacagaga agcccugugu aaguggauag auuucacgag ggacuuucuu     2100

ucuaaauaca ccaagacuac aucaauugau uugaguagcc uccggccauc cucucaguau     2160

aaagaccugg gugaguacua ugcugagcua aacccucugc ucuaccauau cucguuccag     2220

cgcauugccg aaaaggagau cauggaugca guggaaaccg gcaagcugua ucuuuuucaa     2280

auauacaaua aagacuucgc uaaggggcac cauggaaaac ccaacuugca cacacuguau     2340

uggacuggcu uauucagccc agagaaucuc gccaagaccu ccaucaaacu gaacggucag     2400

gcggaauuau uuuacagacc caagucaagg augaaacgua uggcccaccg gcugggggag     2460

aagauguuga auaagaaacu caaggaucag aaaacgccua uuccagacac ccuguaccaa     2520

gagcuauaug auuacgucaa ccaucgacug agucacgacc ucucugauga agcuagagca     2580

cugcuuccga augugaucac aaaggagguu agccaugaaa uuaucaaaga caggcgcuuc     2640

acuuccgaca aguuuuucuu ucacgugccc auaaccuuga acuaucaggc cgcuaauuca     2700

ccuagcaagu ucaaccagcg ggucaaugcc uaccugaaag agcauccaga aacacccauu     2760

aucggcaucg auagaggaga gaggaacuua auuuauauca cugugauuga cucuaccggc     2820

aagauacucg agcagcgcuc ccugaacacg auccaacagu uugauuacca gaaaaagcug     2880

gacaaucgag aaaaagagcg gguagcagcg cgucaggccu ggaguguugu ggggacaauu     2940

aaggaucuua agcaaggaua ucuaagccag gucauccacg aaauugugga ccucaugauc     3000

cacuaccagg cugucguggu ucuggagaac uugaauuucg guuuuaaauc uaagagaacc     3060

ggcauagccg aaaaagcagu guaccagcaa uucgagaaga ugcugaucga uaaauuaaac     3120

ugccucguac ugaaggacua uccugcugag aaggucgggg gagugcuuaa uccauaccag     3180

cugacugauc aguuuacauc cuucgccaaa augggcaccc aaucagguuu cuuguuuuau     3240

guucccgcac cguacacaag uaagauugac ccucucaccg gguucgugga uccauuuguc     3300

uggaaaacaa ucaagaacca ugaaagcagg aaacacuucc uggagggcuu ugacuuccua     3360

cauuaugaug ugaagacggg agacuuuauu cugcacuuca agaugaaucg caaccuuucg     3420

uuucagcggg gccuccccgg guucaugccu gccugggaca ucguauuuga gaaaaaugaa     3480

acccaguucg augcuaaggg aacucccuuu auagcgggua aaagaauugu gccaguuauc     3540

gagaaccaua gguucacagg ccgauaccgc gaccuguauc cugccaauga auugaucgca     3600

cuguuagagg aaaaggggau ugucuuccgg gauggaucca acauacuccc caaacugcuu     3660

gagaaugacg auucucacgc uaucgacacc augguggccc ugauuagaag cguguugcag     3720

augaggaacu caaaugccgc uacaggcgaa gauuacauca acaguccagu ccgugaccuc     3780

aacgguguuu guuuugauuc ccgcuuccaa aauccggagu ggccuaugga cgcagaugcc     3840

aacggggcgu accacauugc ucugaagggc cagcuacugc uuaaucaucu caaagagucu     3900

aaggaccuga aguugcagaa cggaaucagc aaucaggacu gguuagccua uauacaagaa     3960

cugcggaaca aaagacccgc agccacuaag aaagcuggcc aggcaaagaa aaagaaguga     4020


<210>  3489
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3489
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc aagcauuuac       60

caggaguucg uuaacaagua uucccugucu aaaacccuca gguuugaacu uauaccacaa      120

ggaaagacac uggagaauau caaggcccgg gguuugauuu uagacgauga aaaacgcgcg      180

aaggacuaca aaaaggcuaa acagaucauu gauaaguauc aucaguucuu uaucgaggaa      240

auacugucaa gugugugcau cagcgaggac cuacuccaga acuacuccga uguauauuuc      300

aagcugaaaa agucugacga ugacaaucuu caaaaagauu uuaagucggc caaagacacu      360

auuaagaaac agaucucaga guacauuaag gauagcgaaa aguucaaaaa ccuguuuaau      420

cagaacuuga ucgacgcaaa gaaaggccag gagagugauc ucauacugug gcugaagcaa      480

uccaaagaca augggauuga acucuucaag gccaacucug acaucacgga uauugacgag      540

gcuuuagaga ucaucaagag cuuuaaagga uggaccacau auuucaaggg cuuucacgaa      600

aaucguaaaa acguguacuc cucaaaugau auuccgacaa guauaaucua ccgaauuguc      660

gacgauaacc ugcccaaguu ccuugagaau aaagcaaagu augagagcuu gaaggacaaa      720

gccccugagg cuaucaacua cgaacagaua aagaaagauc uggccgagga gcuaaccuuu      780

gacauugauu auaagacauc ugaagugaau cagagaguuu ucucccucga cgagguguuc      840

gaaaucgcga acuuuaacaa uuaccugaac cagucaggua ucaccaaauu caauacuauu      900

aucgggggaa aguuugucaa cggcgagaau acaaagagga aaggcauuaa cgaauauaua      960

aaucuuuaca gccaacagau caacgauaag acccugaaaa aguauaaaau gaguguguug     1020

uucaagcaga uucucucuga cacggagucc aagagcuuug uaaucgacaa acuggaggau     1080

gacucggaug uugugacuac caugcaaucu uucuacgaac agaucgcagc cuuuaagaca     1140

gucgaggaaa aauccauuaa ggagacuuua ucacuccugu ucgacgaucu uaaagcucag     1200

aagcuggacu ugaguaaaau auacuuuaag aaugauaaga gccuaaccga ccugucccag     1260

caaguguucg augacuauuc ugugaucggg acagccgucc ucgaauacau uacccagcag     1320

aucgcaccca aaaaccugga uaauccaagc aagaaagagc aggagcuuau ugcuaagaaa     1380

acggaaaagg ccaaguaucu gucauuggag acuaucaaac ucgcgcugga agaguuuaac     1440

aagcaucggg acauagauaa acaaugucgc uucgaggaaa ucuuagccaa uuuugcagcu     1500

auuccuauga ucuucgacga gauugcccag aacaaggaca aucuggcuca gaucaguaua     1560

aaauaccaga accaaggaaa gaaggaucua cuccaggccu ccgcagaaga cgauguuaaa     1620

gccauuaagg accugcuuga ucagacaaac aauuugcugc acaaacucaa gaucuuccau     1680

auuagccagu cugaggacaa agcuaacauc cuggauaagg acgaacacuu uuaucuugug     1740

uucgaggagu gcuacuuuga acuggcgaau auaguaccau uguauaacaa aaucagaaau     1800

uacauuaccc aaaagcccua cucagaugag aaguucaaac ucaacuuuga aaauaguacu     1860

cuggcaaacg guugggacaa gaauaaagag ccugauaaca cagccaucuu auucauuaag     1920

gacgauaaau auuaccuggg cgugaugaau aagaagaaca auaaaaucuu ugacgacaag     1980

gcuauaaaag aaaacaaggg ggagggauau aaaaagauug ucuacaagcu acucccaggc     2040

gccaauaaaa ugcugccgaa gguguucuuu agcgcaaaau ccaucaaguu cuauaacccc     2100

ucugaggaua uccuuaggau ucggaaccac ucgacccaua cgaaaaaugg uagcccucag     2160

aagggguacg aaaaguuuga guucaacauc gaagacuguc gcaaauuuau ugauuucuau     2220

aagcagucca uaucaaaaca cccagagugg aaggacuucg gauuucgauu cagugauacc     2280

caaagauaca auucuaucga cgaauuuuac agggagguug agaaccaggg cuauaaauug     2340

acauucgaaa auauuagcga guccuacauc gauucagugg ucaaccaggg caagcuguau     2400

cucuuucaga uuuacaauaa ggacuucagc gccuauucua aagggcggcc caaccugcau     2460

acuuuauacu ggaaggcucu guuugaugaa cguaaucuuc aagacguggu auauaaauug     2520

aacggagagg ccgaacucuu cuaccgcaag caguccaucc cuaaaaagau aacccaccca     2580

gcaaaagaag cgaucgcuaa uaagaacaag gauaauccca aaaaggagag uguuuuugaa     2640

uacgaccuga uuaaagacaa gagauucaca gaggauaaau uuuucuucca cugcccgauc     2700

acuauuaacu uuaagagcuc uggugccaau aaguucaacg acgaaaucaa ccuacugcuc     2760

aaagagaagg caaaugaugu gcauauacug uccauugaca ggggcgagcg gcaccuugcc     2820

uauuacaccu uggucgaugg gaaaggaaac aucaucaagc aggacacauu uaauauuaua     2880

ggcaacgauc gaaugaaaac caauuaucau gacaagcugg cugccaucga aaaggaucgc     2940

gacucagcaa gaaaagauug gaagaaaauu aacaauauca aggagaugaa agaagguuac     3000

uuaagucagg ugguccacga gauugcuaag cucgugaucg aauauaacgc cauaguugug     3060

uucgaggacc ugaauuuugg guucaagagg ggccgguuua aaguagagaa gcaagucuac     3120

cagaaacuug aaaagaugcu gaucgagaaa uugaacuauc ucguguucaa ggauaaugaa     3180

uuugacaaaa cuggaggcgu ucugcgcgcg uaccagcuaa cggccccuuu cgagacauuu     3240

aagaagaugg ggaaacagac cggaauuauc uacuaugugc ccgcugguuu cacuagcaag     3300

auuuguccag ucaccggcuu ugugaaccaa cuguacccua aauaugaauc gguauccaag     3360

ucucaggagu ucuucagcaa auuugacaag aucugcuaca aucucgauaa gggguauuuc     3420

gaguuuucau ucgacuacaa aaacuuugga gauaaggcag ccaaaggcaa guggacaaua     3480

gcuaguuucg guuccagacu gauuaauuuu aggaacucug acaaaaauca uaacugggau     3540

acccgugaag uguaucccac uaaggagcuu gaaaaguugc ugaaagacua cagcaucgag     3600

uacgggcacg gcgaguguau caaggccgca auuugcggag aauccgauaa aaaguucuuu     3660

gcgaaauuaa caucaguucu caacacgauc cugcagaugc ggaauagcaa gaccggcacu     3720

gagcuugacu aucugauaag uccagucgcc gaugugaacg ggaauuucuu ugacucucga     3780

caagcuccca agaacaugcc ucaggaugcc gacgcaaaug gagcuuacca cauuggucua     3840

aaaggccuca ugcuguuggg gagaaucaag aacaaucagg aaggcaaaaa gcugaacuua     3900

gugauuaaaa augaggaaua uuucgaguuu guccagaaca ggaauaacaa gcgcccagcc     3960

gcaacuaaaa aggccggaca agcuaagaaa aagaaauga                            3999


<210>  3490
<211>  4716
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3490
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaga caagaaauac agcauuggac uggauauagg uacaaacucc guuggcuggg      180

ccgugaucac agacgaguau aagguaccau cuaagaaauu caaggugcuc gggaauacug      240

auaggcauuc aauuaaaaag aaccuuaucg gagcgcuguu guuugacagu ggcgaaacgg      300

cugaggccac ccgguuaaaa cgcacagcac gacguagaua cacuaggcgg aagaaucgca      360

uuugcuaucu gcaggaaauc uucagcaacg agauggccaa ggucgaugac uccuuuuucc      420

acagacuaga agagucuuuu cucguggagg aagauaaaaa gcaugagagg cacccgauau      480

ucgguaauau cguugacgaa guggcuuacc augagaaaua ucccaccauu uaccaucugc      540

ggaagaaacu ugucgauucg acagacaagg cagaucugcg cuugaucuau cucgcccugg      600

cucacaugau uaaauuucga gggcacuucc ugaucgaggg agaccucaac ccugauaauu      660

cagacgugga caaguuauuu auacaacugg uacagaccua caaccagcuu uucgaagaga      720

auccaauuaa cgccagcggc guugaugcga aggcaaucuu gagugccaga cuguccaaau      780

cuaggcggcu agaaaaucuc auugcucagc ugcccggcga gaagaaaaac gggcuuuuug      840

gaaaucugau cgccuugagc cucggucuga cuccuaacuu caaguccaau uuugacuuag      900

cagaagaugc uaaacugcaa cucucaaagg acacauacga ugacgaucuu gacaaccugu      960

uggcccagau cggcgaucag uaugcggacc uauuccuggc cgcaaagaac cucagugaug     1020

cuauucugcu uagcgacaua cugcguguga auaccgagau cacgaaagcc ccauugucug     1080

cuuccaugau uaagcgcuac gacgagcacc aucaggaucu cacucuguua aaagcccugg     1140

ucagacaaca gcuacccgaa aaguauaaag agaucuucuu ugaccaguca aagaacgggu     1200

acgcaggaua uauagauggc ggugccagcc aggaagaguu cuacaaguuu auuaaaccua     1260

uccucgaaaa gauggacggg accgaggagc ugcuugugaa auugaauagg gaagaucugc     1320

uccggaagca acgaacauuc gacaacggaa guaucccaca ccagauucac cugggcgagc     1380

uucaugcuau ccugcgcaga caggaagauu uuuauccguu cuugaaagac aauagggaga     1440

agauugaaaa gauacucacu uuucggaucc ccuacuacgu gggcccucug gcgcgcggga     1500

acucuagauu cgcauggaug accaggaaau ccgaggagac aauuacccca uggaauuuug     1560

aagaggucgu ugauaaggga gccagcgcuc aaucguucau cgaacguaug acgaacuuug     1620

acaaaaauuu acccaacgag aaggugcugc cuaaacacuc ucuacucuau gaguacuuca     1680

cuguauauaa ugaacugaca aaggugaaau acgucaccga ggguaugcgg aagccagccu     1740

uucuuuccgg cgaacagaag aaagcaaucg uggauuugcu guucaagacu aaccgaaaag     1800

uuacagugaa gcagcucaaa gaggacuauu ucaagaagau ugaauguuuu gauucagucg     1860

agauaagugg gguggaggac agauucaaug ccagccuggg aaccuaccau gacuuacuga     1920

aaaucauuaa ggauaaagac uuucuugaua acgaagagaa ugaagacauc uuggaggaua     1980

uuguacucac gcugacccua uucgaagaca gggagaugau cgaggaacgc cugaagacau     2040

augcucaccu cuuugaugac aaaguuauga agcagcugaa gcggagaagg uacacuggcu     2100

ggggucgccu uucccgaaaa uugauaaacg ggauccggga uaagcaaucu ggcaaaacca     2160

uucuggacuu cuuaaagagc gauggauuug ccaaccguaa uuucaugcag cucauccaug     2220

acgauucacu gacauuuaaa gaggacauuc agaaggcaca ggugaguggc caaggggacu     2280

cccuucacga acacaucgcg aaccuggcug gaagccccgc cauaaagaaa gguauuuugc     2340

agacugucaa gguggucgau gagcucguga aaguuauggg cagacauaag ccggaaaaua     2400

ucgugauuga gauggcaagg gagaaccaga ccacacagaa agggcaaaag aacucucgcg     2460

aacggaugaa aagaaucgag gaaggaauaa aggagcuggg cucacagauc cuaaaggagc     2520

acccuguaga aaauacccag cugcagaaug agaaacucua ccuguauuac cuucaaaacg     2580

guagggacau guaugucgau caggaauugg acauuaaucg acugagugau uacgacgugg     2640

aucauaucgu uccccagagc uucuuaaagg acgauuccau ugacaacaaa gugcucacuc     2700

gcucugauaa gaaucggggg aaaucggaca acgucccaag cgaggaagug guaaagaaga     2760

ugaaaaauua uuggagacaa cugcuuaacg ccaagcugau cacgcagagg aaauuugaca     2820

aucuaacaaa ggcugagcgu ggcggacucu ccgagcugga uaaagccggc uucauaaagc     2880

gccaguuggu ggaaacccgg cagauuacua agcacguugc acaaauccug gacucaagaa     2940

ugaacaccaa auacgaugag aacgacaagu uaaucaggga agucaaagug auuacacuca     3000

agaguaaacu ggugucugau uuucgaaagg acuuccaguu cuauaagguc cgggagauca     3060

auaacuacca ccaugcucac gaugccuacc uuaaugcggu uguggggacc gcccugauua     3120

aaaaguaucc uaaauuggaa agcgaguuug uguacggaga cuauaaggua uacgaugucc     3180

gcaaaaugau agcuaagucc gagcaggaaa ucgguaaggc aacugccaaa uauuucuuuu     3240

acucaaacau uaugaauuuc uuuaagacag agaucacgcu cgcuaacggc gaaauuagaa     3300

aaaggccccu gaucgagacc aauggggaaa cuggagagau agugugggac aagggccggg     3360

auuucgccac aguucgaaaa gugcuaagca ugccacaggu caacaucgug aagaaaaccg     3420

agguucaaac agguggguuu ucuaaggaau ccauucugcc caagagaaau agugacaaac     3480

uuaucgcacg caagaaagau ugggacccua agaaauaugg cggauucgac agcccaacug     3540

uggcguacuc uguacucguc guggccaagg uggagaaggg caaauccaag aaacugaagu     3600

cagucaaaga auugcugggg auuaccauca uggagaggag uagcuuugag aagaacccga     3660

uagauuucuu agaagcuaag ggauacaaag agguuaagaa agaccucauu aucaagcugc     3720

ccaaauauuc gcuuuuugaa cuggagaaug gccguaagcg gauguuggcc uccgcuggug     3780

aacuccagaa ggggaacgag cuggcacuac cuucuaaaua cgugaauuuc cuguaucuug     3840

ccagccauua cgagaagcuc aaaggaucac cagaagauaa cgagcagaag cagcuguucg     3900

uagaacaaca caaacauuau uuggacgaga ucauugaaca gauaagugag uuuuccaaga     3960

gagugaucuu agcagaugcc aaucuggaca aaguccucuc ugcuuacaac aagcacaggg     4020

auaagcccau ucgcgagcag gcggaaaaca ucauucaccu guucacgcuu accaaucugg     4080

gcgccccugc agcuuuuaaa uauuucgaca caacuaucga ucgaaagaga uacaccagca     4140

caaaagaggu guuggacgcc acucuaauac aucaauccau caccggucug uacgaaacgc     4200

ggauugaucu cucacagcug ggcggggaca agaggccagc cgcuaccaaa aaggcaggac     4260

aggccaagaa aaagaaauaa ggacuaguga agggaacacc caaauuuaau ucagccuuaa     4320

gcacaauuaa uuaagaguga aacguaauug uacaagcagu uggucaccca ccauagggca     4380

ugaucaacac cgcaaccuuu ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu     4440

cagcuugcuu agauguucca aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa     4500

aaaaaggcca caaaaguucc cucucacuuu caguaaauaa aauaaaagca gcaacagaaa     4560

uaaagaaaua aaugaaauuc aaaaugaaau aaauauugug uugugcagca uuaaaaaauc     4620

aauaaaaauu aaaaaugagc aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               4716


<210>  3491
<211>  5499
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3491
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa cuucaagauu cugccaauag ccaucgaccu cggaguuaaa aauaccggug      180

uguuuagcgc guucuaccag aagggcacau cccuugagag gcuggauaac aagaauggga      240

aaguauauga auugucuaag gacucauaca cuuuacugau gaacaaucgg acggcucgcc      300

gacaucaacg uagaggaauu gauaggaaac agcuagugaa gcggcucuuu aaacugaucu      360

ggaccgagca gcuuaaccug gaaugggaca aggauacaca gcaagccauu aguuucuugu      420

uuaaucgcag aggcuucagc uuuaucacug acgguuauuc cccggaguac cucaacauag      480

uccccgaaca ggugaaggca auccugaugg auauuuucga cgauuauaau ggggaggacg      540

aucuggacuc uuaccucaaa uuagccaccg agcaggaauc gaagaucuca gagauuuaua      600

acaaacugau gcagaagauc cuugaauuua aauugaugaa gcugugcaca gauauaaaag      660

acgacaaggu uagcaccaag acucuaaaag agauuacaag uuacgaguuc gaacuccugg      720

cugauuaccu ugcaaauuau uccgagucuc ugaagaccca aaaauuuagc uacacggaca      780

agcagggaaa cuugaaagaa cucuccuauu accaccauga uaaguauaau auccaggagu      840

uccugaagag gcacgccacu auuaacgacc ggaucuuaga uacccugcuc acagacgauc      900

uugacaucug gaauuuuaac uucgaaaaau ucgauuuuga caagaacgag gagaaacugc      960

agaaucaaga agauaaggac cauauucagg cucacuugca ccauuucgug uuugccguca     1020

acaaaauaaa gucagagaug gcgaguggcg gccgccaccg aagccaguac uuccaagaaa     1080

ucacuaaugu gcuagacgag aacaaucauc aggaagggua ucugaagaac uuuugugaga     1140

aucuccacaa caaaaaguac ucuaaucugu ccguuaaaaa ccuuguaaau cugauuggaa     1200

acuugucaaa ucucgagcug aagccuuuaa gaaaauacuu caacgauaag auccacgcaa     1260

aggccgacca uugggaugaa cagaaauuua ccgagacaua uugccacugg auacugggug     1320

aauggagggu gggcgucaag gaccaggaua aaaaggacgg ggcuaaauac agcuauaagg     1380

aucuauguaa ugagcucaaa caaaagguga ccaaggccgg acugguggac uuccuuuugg     1440

aacuggaucc augccggacg auucccccuu accucgacaa caacaaucgu aaaccaccca     1500

agugucagag ucugauccuu aacccuaaau uucuggauaa ucaguaucca aacuggcagc     1560

aauacuugca ggagcucaag aaacugcagu cuauccagaa uuauuuagac uccuucgaga     1620

cugaucugaa gguccuaaag agcucgaaag accaaccgua cuuuguugaa uacaagucuu     1680

ccaaccagca gauugcauca ggccagcgcg acuauaaaga ucucgacgcu agaauccugc     1740

aauucauuuu ugauagggug aaggccagug acgagcuuuu gcugaaugaa auauacuucc     1800

aggcgaaaaa gcucaagcag aaagccagcu ccgagcugga gaaguuagaa ucuagcaaaa     1860

agcuggauga gguaaucgca aacucacaac uuagucagau uuugaaaucc cagcauacaa     1920

augguaucuu cgaacagggg accuuucucc accuggugug caaguauuac aagcaacggc     1980

agcgagcucg cgacagcaga cuauauauca ugcccgagua cagguaugau aaaaagcugc     2040

auaaauacaa caauacugga cgguucgacg augacaacca gcuccugaca uacuguaauc     2100

acaagccucg ccagaaaaga uaucaacuuu ugaacgaucu ggccggcguc uuacaggugu     2160

cuccaaauuu ucucaaggac aaaauuggcu cagaugacga ccuguucaua aguaaguggc     2220

uuguugaaca caucaggggg uuuaagaaag caugcgagga uagccugaag auucagaaag     2280

acaaccgugg auugcucaac cauaagauca auauugcccg gaacaccaaa gguaagugug     2340

agaaggaaau cuucaaucug auaugcaaaa ucgagggcuc cgaagauaag aaagggaacu     2400

acaagcacgg acuagcauau gagcugggcg ugcuccuguu uggugaaccc aaugaggccu     2460

cuaaaccuga guucgaccga aagauuaaga aauuuaacuc gaucuacagc uucgcucaga     2520

uucaacagau cgcguuugca gaaagaaagg ggaaugccaa cacgugugcu gucugcuccg     2580

ccgauaaugc acauaggaug cagcaaauaa aaauuaccga gccaguggaa gacaacaagg     2640

auaaaaucau ucuuucagcc aaggcucagc gcuugcccgc caucccgaca cggauaguag     2700

acggcgcagu uaagaaaaug gcgacuaucc uggcuaagaa uauuguggau gacaacuggc     2760

agaauaucaa acaggucuua agugccaagc accaacucca cauuccuauc auaaccgagu     2820

cuaacgcauu cgaauuugag cccgcccugg cugaugugaa aggaaagagc cuuaaggaca     2880

gaaggaaaaa ggcccuggag cgcauuuccc cagaaaauau cuucaaagac aagaacaacc     2940

gaaucaaaga guucgcaaag ggcauuucag cuuauagcgg ggccaaucua acagauggag     3000

acuuugaugg ugcgaaagaa gaacucgacc auaucauucc ucggucucac aagaaguacg     3060

gcacucugaa cgaugaggcc aauuugauau gcgucacccg uggggacaac aaaaauaagg     3120

gaaacagaau cuucugucug agggauuuag cugacaauua uaaacucaag caguuugaaa     3180

caaccgauga ccuggagauu gagaaaaaga ucgcagauac uauuugggac gccaacaaga     3240

aagauuucaa guuuggcaau uaccgcuccu ucaucaaccu uacgccccag gagcagaagg     3300

cuuuucggca ugcccuguuc uuggcagacg aaaauccaau aaaacaagcg gugaucagag     3360

ccauuaacaa uaggaaccga acauuuguua augguaccca gcgcuacuuc gcugaggugc     3420

ucgccaacaa uaucuaucug cgggcuaaaa aggagaaccu aaacacugac aagauuaguu     3480

uugauuacuu cgggaucccc accauaggca auggaagagg cauugcugaa aucaggcagc     3540

uguaugagaa aguagacagc gauauccagg ccuacgcaaa gggggacaaa ccucaagccu     3600

cuuauuccca ccuuauugau gcuaugcucg cguucugcau agccgcagac gaacaucgua     3660

acgauggauc aaucggucug gagauugaca agaauuacag uuuguaucca cuggauaaaa     3720

acacaggcga agucuuuacc aaggacaucu ucagccagau uaagaucacu gauaaugagu     3780

uuucggacaa aaaguuagug cgcaaaaagg cuauagaggg guucaacaca caccggcaga     3840

ugacgagaga uggaaucuac gccgaaaauu accucccgau ucugauccac aaagagcuua     3900

acgaaguuag gaagggcuau accuggaaaa auuccgagga aauuaagauc uuuaagggua     3960

aaaaguacga cauacagcaa cugaacaauu ugguguauug ucucaaauuc gucgacaagc     4020

ccauuucuau cgauauccag auuagcacuc uggaggagcu acgaaacauc cugacaacca     4080

auaacauagc cgcuacagca gaauacuauu acauuaaucu uaaaacucag aagcuccaug     4140

aguauuacau cgaaaacuac aacaccgccc ugggguauaa gaaauacuca aaggagaugg     4200

aguuuuugcg gaguuuagcg uaucgcuccg aaagagugaa aauuaagucu aucgacgaug     4260

uaaaacaagu gcuggacaag gauagcaauu ucauaauugg caagaucacg cucccuuuua     4320

aaaaggagug gcagaggcug uaccgggaau ggcagaacac cacaaucaaa gacgauuaug     4380

aguuccuuaa guccuuuuuc aauguuaaau caauuacuaa gcugcacaag aaaguccgaa     4440

aggacuuuag cuugccaauc aguaccaacg aaggaaaauu ccuagugaag agaaaaacau     4500

gggauaauaa cuucauuuac cagauacuga augacucuga uucccgcgcu gacggcacua     4560

agcccuuuau cccugccuuc gacauuagca aaaacgagau cguggaggca aucauugauu     4620

cguuuaccuc aaagaauaua uucuggcucc caaagaacau cgaacugcaa aaagucgaca     4680

auaagaacau uuuugccauc gauacgucua aaugguucga gguugaaaca cccagugacc     4740

uuagggauau ugggaucgcu accauacagu auaagaucga caauaacucc cguccgaaag     4800

ugcgggugaa guuagauuac guaauugacg augacagcaa gaucaauuac uuuaugaacc     4860

auucucugcu caaaagcaga uauccugaua aggucuugga gauucugaaa caguccacua     4920

ucauagaguu cgaaucaagu ggauuuaaca agacaauuaa agagaugcuc ggcaugaagc     4980

uggcagguau cuacaaugaa accagcaaca auaagaggcc agccgcgacu aaaaaggcug     5040

ggcaggccaa aaagaaaaag uaaggacuag ugaagggaac acccaaauuu aauucagccu     5100

uaagcacaau uaauuaagag ugaaacguaa uuguacaagc aguuggucac ccaccauagg     5160

gcaugaucaa caccgcaacc uuuccuuuuu cccccaguga uucugaaaaa ccccucuucc     5220

cuucagcuug cuuagauguu ccaaauuuag uaagcuuaag gcggccuaca gaagaaaaag     5280

aaaaaaaagg ccacaaaagu ucccucucac uuucaguaaa uaaaauaaaa gcagcaacag     5340

aaauaaagaa auaaaugaaa uucaaaauga aauaaauauu guguugugca gcauuaaaaa     5400

aucaauaaaa auuaaaaaug agcaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5460

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            5499


<210>  3492
<211>  3771
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3492
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaggaacuac auucugggac ucgacauagg uaucacauca guuggcuaug      180

ggauuaucga uuacgagaca cgggacguga uugaugccgg aguacgccuu uucaaagaag      240

cgaaugugga gaacaaugaa ggccgacguu ccaagagagg ugcuaggcgg cugaagcgca      300

gaaggcggca ucgcauccag cgagucaaaa aguuguuauu ugacuauaac cugcuaacug      360

aucacucuga gcucucaggg auaaauccau acgaagccag agugaaagga cugagucaaa      420

aacuuagcga ggaggaguuc uccgcagccc uguugcaucu cgcuaaaagg cggggcguuc      480

acaacgugaa ugaagucgaa gaggacacgg gcaacgagcu gucuaccaag gaacagaucu      540

cgcguaauuc aaaggcacug gaggaaaaau auguggccga gcuccaguua gaacgccuga      600

agaaagaugg ggagguaaga ggaagcauua acagguuuaa gacaagugac uacguuaaag      660

aggcuaagca gcuuuugaaa gugcaaaagg ccuaucauca gcuggaucag uccuucaucg      720

acacuuacau ugaucuacuc gaaacccggc gaacauacua ugaggguccg ggcgaagggu      780

cucccuuugg auggaaggac aucaaagagu gguacgaaau gcugaugggc cacugcaccu      840

auuucccuga ggagcuucgc agcgucaagu acgcguauaa ugcagaucug uacaacgccu      900

ugaaugaccu caacaaucug gugauaacua gagacgaaaa cgagaaauua gaauauuacg      960

agaaguuuca gauuaucgaa aacguguuca aacaaaagaa gaaaccaaca cugaagcaga     1020

uugcuaaaga gauccucguc aaugaggaag auaucaaggg uuacaggguu accuccacgg     1080

ggaaacccga guuuacuaac cuuaaggugu aucacgacau uaaggauaua accgcccgga     1140

aagaaaucau ugagaaugca gagcuguugg accagaucgc uaagauacua acaauuuacc     1200

agucaaguga agauauccaa gaggaacuga cuaaccucaa uagcgagcug acccaggaag     1260

agaucgagca gauuucuaac cuuaaaggau auacaggcac ccauaaucug uccuugaagg     1320

ccaucaaccu cauucuggac gaauuauggc acacgaauga uaaccaaaua gcgaucuuca     1380

aucgccugaa acuuguaccu aagaaggugg accucucaca gcagaaagag auuccaacua     1440

cacuggucga ugacuuuauc cuuagccccg ugguuaagag aaguuucauc cagucuauua     1500

aagugauaaa cgccaucauu aagaaauacg gcuugccuaa ugauaucauu aucgaacugg     1560

caagggagaa gaacuccaaa gacgcucaaa agaugauaaa ugaaaugcag aagcguaacc     1620

ggcagaccaa cgagcgaauc gaggaaauua ucagaacuac agggaaagag aaugccaagu     1680

aucucauuga aaagaucaag cugcaugaca ugcaggaggg aaaaugucuu uacagccugg     1740

aagcuauacc auuggaggau cuccugaaca auccguuuaa cuaugagguc gaccacauua     1800

uccccagguc ggugucuuuc gauaauuccu ucaacaauaa gguauuaguu aagcaagaag     1860

agaacucaaa gaaggguaau cgcaccccuu uucaguaccu gaguagcucc gacucuaaaa     1920

uuagcuacga aacguucaag aaacacaucc uaaaccucgc caagggcaag gggcggauau     1980

caaaaaccaa gaaagaguau cugcuugagg aaagagauau caauagguuu agugugcaga     2040

aggacuucau uaaccgcaau uuggucgaua cacgauacgc aacucgggga cugaugaacc     2100

uccugcguuc cuauuuuaga gugaauaacu uagacgucaa agugaaaagc aucaacggcg     2160

guuucaccuc uuuucugagg cgcaagugga aauucaagaa agagcggaau aagggguaca     2220

aacaucacgc cgaagaugcu cuuauuaucg cgaacgcaga cuuuauauuc aaggagugga     2280

aaaaguugga uaaggccaaa aagguuaugg aaaaucagau guuugaggag aaacaagcug     2340

aaucaaugcc agagauugaa acagagcagg aauauaagga gaucuucauc acuccccauc     2400

agauuaaaca caucaaggac uuuaaggauu acaaauauag ucauagagug gacaagaaac     2460

cuaacaggga gcucauuaau gacacccugu acagcacacg aaaggaugac aaaggcaaca     2520

cccuaauagu aaauaaccug aauggacucu acgauaagga caacgauaag cugaaaaagc     2580

uaaucaauaa auccccagaa aaguugcuga uguaucacca cgacccccag acuuaccaaa     2640

aauuaaagcu cauuauggag caguauggcg augaaaagaa cccgcuguac aaauauuacg     2700

aggaaacggg gaauuaucuu acaaaguacu cuaaaaagga caacggaccu gucaucaaaa     2760

agauuaagua cuaugguaau aaacugaacg cccauuugga uaucaccgac gauuacccca     2820

acucgcgcaa uaaggugguu aaacucagcc ugaagccaua ucgguucgac guguaccuag     2880

auaacggcgu cuauaaauuc gugacuguaa agaaucugga cgugaucaaa aaggagaacu     2940

acuaugaggu uaauuccaag ugcuacgaag aggccaaaaa gcucaaaaag auaucaaacc     3000

aggcagaauu uauugcuagu uucuacaaua acgaccugau caaaauuaau ggggagcuuu     3060

auagagucau cggagugaac aaugauuugc ugaacaggau agaggugaau augauugaca     3120

ucaccuaccg ugaauauuua gagaacauga augauaagcg cccuccccgg aucauuaaga     3180

caauagccuc uaaaacccaa agcaucaaga aauacuccac ugacauucuc ggcaaccugu     3240

augaagucaa gagcaaaaag cacccacaga ucauuaagaa agguaagaga cccgcagcga     3300

ccaaaaaggc ugggcaggcc aaaaagaaga aauaaggacu agugaaggga acacccaaau     3360

uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa gcaguugguc     3420

acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu gauucugaaa     3480

aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua aggcggccua     3540

cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua aauaaaauaa     3600

aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua uuguguugug     3660

cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              3771


<210>  3493
<211>  4839
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3493
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcacu guucaacaag ugcauuauaa ucagcauuaa ucucgacuuu uccaacaaag      180

agaaguguau gaccaagcca uacucuaucg gacuugauau ugguacaaau ucaguuggcu      240

gggccgugau cacugacaac uauaaaguac cgaguaagaa aaugaaggug cuggggaaua      300

cgagcaaaaa guacauaaag aaaaacuugu uaggaguccu gcuauucgau uccggcauca      360

ccgcggaagg uaggcggcuc aagcgcacag cucgacguag auauacuagg cggcgcaaua      420

ggauucugua ccuucaggag aucuuuucua ccgaaauggc cacacuggac gaugcauucu      480

uucaaagauu ggacgauucg uuccucgugc ccgacgauaa acgggacuca aaguauccua      540

uuuuugggaa ccugguugag gaaaaagugu accaugauga guucccaacc aucuaucauc      600

ugcgcaagua ccucgccgac agcacuaaaa aggcugauuu acgacugguc uaccuugcau      660

uggcccacau gauaaaguau agaggacacu uucugauuga gggcgaguuc aauaguaaaa      720

acaaugacau ccagaagaac uuucaggacu uccuagauac auacaaugcu auuuuugagu      780

ccgaccucuc ucuggaaaac agcaaacagc uugaggagau cgugaaggau aaaaucucca      840

agcuggaaaa gaaagacagg auuuugaaac ucuuccccgg cgagaaaaau ucagggauau      900

ucagugaauu ucugaaguua aucguaggaa accaagccga uuuccggaaa ugcuuuaacc      960

uggacgagaa ggcgagccuc cauuucucua aggaauccua ugaugaggac cuugagaccc     1020

uguuggguua cauuggcgau gacuauucag auguuuuucu aaaagcaaag aaacuguacg     1080

acgccauccu ccugagcggg uuccuuacgg ugacugacaa ugaaaccgag gcuccucuga     1140

guucugccau gauaaagcgu uauaacgaac acaaagagga uuuggcacuc cugaaggaau     1200

acauucgcaa uaucuccuua aagacauaca acgaggucuu uaaagacgau acuaagaaug     1260

gauaugcugg cuacaucgac gguaaaacca accaggagga uuucuaugug uaccugaaga     1320

aucuacucgc cgaauuugag ggggcggacu auuuccugga aaagauugau agagaggacu     1380

uucuuaggaa gcagcggaca uucgauaacg gaagcauccc auaccagauu cacuugcaag     1440

aaaugcgagc cauacuggac aaacaggcaa aguuuuaucc cuuccucgcu aagaauaaag     1500

agcgcaucga gaagauucug accuucagaa ucccuuacua cgugggccca cuugccaggg     1560

gcaacucgga uuuugcuugg ucuauccgga aacgcaauga aaagauuacg ccguggaacu     1620

ucgaggacgu cauagauaaa gaauccucag ccgaggcauu uaucaauaga augacaaguu     1680

ucgaccugua uuugcccgag gaaaagguuc ucccuaagca uagccuguua uacgagacuu     1740

uuaacgugua uaaugaacug accaaaguaa gguucauugc cgaguccaug cgugacuacc     1800

aguuucuaga uucuaagcag aaaaaggaca ucgugcggcu cuauuucaaa gauaagcgaa     1860

aggucacuga caaagauauu aucgaauacc ugcacgcuau auauggguac gacggaaucg     1920

agcuuaaggg uauugagaaa caauuuaaca gcucauugag uacauaccau gaucugcuca     1980

acaucauuaa ugacaaggag uuccuggaug acuccagcaa cgaagcgauc auagaagaga     2040

uuauccacac cuuaacgauu uuugaagaua gagagaugau caaacagagg cugucuaagu     2100

ucgagaauau auuugacaag ucagugcuua aaaaguugag ucgccggcac uauaccggcu     2160

gggggaaacu cagcgcaaag cugaucaacg gaauuagaga ugaaaaaucc ggcaauacaa     2220

uccuagacua ccugauugac gaugguaucu cuaacaggaa uuucaugcag cucauacaug     2280

acgaugcccu gucguucaag aaaaagauuc aaaaggcuca gaucaucggg gacgaggaua     2340

aaggcaacau uaaggaaguu gugaaaagcc uuccaggauc ccccgccauc aagaaaggca     2400

uuuugcaguc aauaaagauc gucgacgagc uggugaaggu aaugggaggg cgcaaaccug     2460

aaaguauugu uguggagaug gcacgagaga aucaguauac uaaccaaggu aagucuaaua     2520

gccagcagcg guuaaaacgu cucgaaaagu cccugaaaga gcuuggcuca aagauccuga     2580

aggaaaacau uccagccaaa uugagcaaga ucgauaauaa cgcucuccag aaugacagac     2640

uguaccuaua uuaccugcaa aacgggaaag auauguauac cggagacgau cucgacauag     2700

acaggcuguc uaacuacgau aucgaccaca uuauccccca ggccuuucuu aaggauaauu     2760

ccauugacaa caaagucuug gugaguagcg caucuaaucg cggcaagucc gaugacuucc     2820

cgucacugga ggucgugaag aaacggaaga cauuuuggua ccaguuacuc aaaaguaagc     2880

ugaucagcca gagaaaauuc gauaaccuua cuaaggcgga gagggguggg cugcuaccug     2940

aagacaaggc uggcuuuaua caacgacagc ucguugagac ccgccagauu acaaaacaug     3000

uggcccggcu guuggaugaa aaguucaaua acaaaaagga cgagaauaac agagcaguaa     3060

ggaccgucaa aaucaucacu cugaagucga cguuaguguc ccaguuucgu aaagauuucg     3120

aacucuauaa gguucgcgag auuaaugacu uucaccaugc ccacgaugcu uaccugaacg     3180

ccgugauagc aucugcccuu cugaagaaau aucccaaguu ggagccagag uucgucuacg     3240

gagacuaucc uaaauacaau agcuuucggg aaagaaaguc agcuacagaa aaaguguauu     3300

ucuacaguaa caucaugaau auuuucaaga aguccaucuc ucucgcggac ggcaggguaa     3360

uugagcgacc ccugaucgaa gugaacgagg agaccgggga aagcguuugg aauaaagagu     3420

ccgaucuagc cacuguccgg cgcgugcugu cauacccaca agugaacguc guuaagaaag     3480

uggaagaaca gaaccacgga cuugacagag guaagcccaa aggccucuuu aaugcuaacc     3540

ugagcaguaa gccuaagcca aauucuaacg aaaauuuggu gggggcaaaa gaguaucugg     3600

auccgaagaa auacggaggc uaugccggua uauccaacag cuucgcugua uuagucaagg     3660

ggaccaucga gaaaggcgcc aagaagaaaa uuacaaaugu gcucgaauuu cagggaaucu     3720

cgauucugga caggaucaac uaccggaagg auaaacuuaa uuuccuguug gagaagggcu     3780

auaaagacau agaacucauu aucgagcugc ccaaguacuc acuauuugag cugucugaug     3840

ggagucgaag aaugcuugca uccauccuca gcaccaacaa uaaacgcgga gaaauucaua     3900

agggcaacca aaucuuccug ucucagaagu uuguuaaauu guuauaucac gcgaagagga     3960

uaagcaauac uauuaacgag aaucaucgua aauacgugga aaaccacaag aaagaguucg     4020

aggaacuguu uuacuauauc cucgaguuca augaaaacua cgucggugcc aagaagaacg     4080

ggaaacugcu uaauuccgcu uuucagucau ggcagaacca caguauugac gagcugugua     4140

gcucuuucau cggaccuaca ggcuccgaac ggaaggguuu guuugagcua acgucaagag     4200

gcagugccgc agauuucgag uuccuggggg ugaaaauacc aagguaucgc gacuacaccc     4260

ccagcucccu ccugaaggau gcuacucuua uucaucaauc uguuacagga uuauaugaaa     4320

cccgaaucga ccuggccaaa cucggcgagg ggaagagacc ugcagccaca aagaaagcug     4380

gacaggcgaa gaaaaagaaa uaaggacuag ugaagggaac acccaaauuu aauucagccu     4440

uaagcacaau uaauuaagag ugaaacguaa uuguacaagc aguuggucac ccaccauagg     4500

gcaugaucaa caccgcaacc uuuccuuuuu cccccaguga uucugaaaaa ccccucuucc     4560

cuucagcuug cuuagauguu ccaaauuuag uaagcuuaag gcggccuaca gaagaaaaag     4620

aaaaaaaagg ccacaaaagu ucccucucac uuucaguaaa uaaaauaaaa gcagcaacag     4680

aaauaaagaa auaaaugaaa uucaaaauga aauaaauauu guguugugca gcauuaaaaa     4740

aucaauaaaa auuaaaaaug agcaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            4839


<210>  3494
<211>  3915
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3494
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaug guacgccagc cugauguccg cgcaucaccu caggguugga auugacgugg      180

guacccauuc uguaggccuu gcuacacugc ggguggauga ccacgggacu ccaauagagu      240

uguuaucagc ccugagucau auccacgaua gcggagucgg caaggaaggu aaaaaggacc      300

acgauacgcg caagaaacua uccgggauug cacgacgugc cagaaggcuc cugcaucacc      360

ggcgcaccca gcuucaacag cuggacgagg uguugagaga ucucggauuc ccgaucccca      420

caccuggcga auuucuggac cugaacgagc agacugaucc auauaggguu uggcgggugc      480

gcgcucgacu cgucgaagag aaguuacccg aggaacugag aggcccugca auuucuaugg      540

ccgugaggca uaucgcucgg caccgugggu ggcgcaaucc auacucgaaa guagagucac      600

uuuugagccc cgccgaagag aguccuuuca ugaaggcgcu gagagagagg auacuagcaa      660

ccacaggaga aguucucgac gaugguauca ccccaggcca ggccauggcu caaguggccc      720

ugacucacaa cauuuccaug cgggggccgg agggaauccu uggcaaacug caucagucug      780

acaaugcaaa cgaaauucga aagaucugcg cucgccaggg ugucagcccc gaugugugua      840

agcaguugcu cagagccgug uuuaaagcgg acuccccuag ggggucagcc gucagucggg      900

uugcaccaga cccccugccu ggacaaggca gcuuccgcag agcuccaaag ugcgaucccg      960

aguuucagag guuccguaua auuucuaucg uggccaauuu acggauuucc gaaacaaaag     1020

gcgagaaccg accgcugacc gcugacgaga gaaggcacgu agugacguuu cucacugaag     1080

auucacaggc cgaccuuacc ugggucgaug uggcagagaa gcuggggguu caucgccggg     1140

acuugagagg aacagccgug cacacugaug acggugaaag gagcgcugcg cgcccuccca     1200

ucgaugcaac cgaccgaauc augcggcaga caaaaauuag uucucuaaag accugguggg     1260

aggaagccga uuccgagcaa cguggcgcua ugauaagaua ucuguacgag gacccaacgg     1320

acagcgaaug ugccgagauc auugcagaac ucccugagga agaucaggcc aaacuggacu     1380

cgcuucaucu gcccgcuggg agggccgcau auucucgcga guccuugacu gcgcucucag     1440

aucacaugcu ggcuacaacc gacgauuuac acgaggcccg gaagagacug uucggagucg     1500

acgauaguug ggcaccaccc gccgaagcua ucaaugcccc ugugggcaac ccaagcguag     1560

acaggacucu aaagauaguu ggucgauacc ucuccgcagu ggagucuaug ugggggacac     1620

cggaagucau ucauguggag cacguccgcg auggcuuuac cagcgagcgg auggcugacg     1680

aaagagauaa agccaauagg cgucgcuaua acgacaauca ggaggcgaug aagaaaaucc     1740

aacgggauua cggaaaggaa ggcuacaucu caagagggga cauugugagg cuggacgccc     1800

uugaguugca gggaugcgcu ugucuguauu gcgguacgac caucggcuac cauacauguc     1860

agcucgauca cauuguuccc caggcagggc cuggaaguaa caaucgacgg ggcaaccugg     1920

uggccguaug cgaacgcugu aauagaucca aaagcaacac uccauucgcu gucugggccc     1980

aaaagugcgg uauaccccac guggggguua aggaggcaau cggcagggug cggggauggc     2040

gaaaacagac cccuaacaca ucuucagagg accuuacuag acugaagaaa gaagucauug     2100

cgcgcuugag gcguacccag gaggauccag aaaucgacga gcggaguaug gaaagcgugg     2160

ccuggauggc uaaugagcuc caucacagaa ucgccgcagc uuaucccgag acaaccguaa     2220

ugguguacag gggcuccauu acugccgcag cccgcaaggc ugcggggaua gauucucgaa     2280

ucaaccugau uggagaaaaa gguagaaagg accggaucga uaggcgccau cacgccguug     2340

acgcaucggu cguggcuuua auggaggcca gcguggccaa gacgcuggcu gaacguuccu     2400

cacuaagagg cgagcagcgg cucacaggga aagaacaaac cuggaagcag uauacuggaa     2460

guaccgucgg cgcaagggag cauuuugaga uguggcgcgg ucacaugcug caccuuacag     2520

aauuguucaa ugagcgacug gccgaagaua aaguuuacgu gacccagaac auuagacucc     2580

ggcugucuga cgggaaugcg cauacuguga acccgagcaa guuaguaucc cacaggcugg     2640

gcgauggacu uacaguccag caaaucgacc gcgcuuguac gccugccuug uggugcgcac     2700

ucaccagaga gaaagauuuu gacgagaaga auggccugcc agccagggaa gaccgggcua     2760

uacgugugca ugggcacgag aucaagucaa gcgauuauau ucagguuuuc ucuaaacgca     2820

agaaaacuga cuccgaucga gacgaaacac ccuucggagc aaucgccgug agaggcgguu     2880

uugucgagau ugggccuagu auccaucacg cgaggauaua ccggguggaa ggaaagaaac     2940

ccguuuacgc uaugcuacgc guguucaccc acgaucugcu cagccagaga cauggcgacc     3000

uguuuucugc cguaauucca ccucagucca ucucaaugag gugcgcagag cccaaacuuc     3060

ggaaagccau uacaacuggu aacgcuaccu auuugggcug ggucguggug ggggaugagc     3120

uggaaaucaa ugucgacagu uucacgaagu acgccauagg acgauuuuua gaggauuucc     3180

caaacaccac aagauggcgc aucuguggcu augacacuaa uagcaagcuc acccugaaac     3240

cgauuguucu ugcagcugaa gggcuggaga acccuucguc cgccgugaau gaaaucguag     3300

aguugaaggg auggagggug gcgauuaacg uccucacaaa agugcacccc acuguugugc     3360

ggcgugaugc ccugggcaga ccaagguacu cuagccgcuc aaaucuaccc accaguugga     3420

cgaucgagaa gcggccugca gcuacaaaga aggccgguca agcuaagaaa aagaaauaag     3480

gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau uaagagugaa     3540

acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc gcaaccuuuc     3600

cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa     3660

auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc     3720

ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa augaaauuca     3780

aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua aaaaugagca     3840

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3900

aaaaaaaaaa gaauu                                                      3915


<210>  3495
<211>  4776
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3495
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaac caagccauac agcauuggac uggacauagg uacaaacucc guuggcuggg      180

ccgugacuac ggauaauuau aaaguaccgu cuaagaagau gaaagugcuc gggaacaccu      240

caaagaaaua caucaagaaa aaucuucugg gagucuuguu auucgacagu ggcauuacag      300

cggaggguag gcggcugaag cgcacugcuc gacguagaua uaccaggcgg cgcaacagaa      360

uccuauaccu ccaggaaauu uuuagcacag agauggccac ccuggaugac gcauucuuuc      420

aaaggcuuga ugacuccuuc cuggugcccg augacaagcg ggauucuaaa uauccuaucu      480

uugggaauuu gguugaagag aaggccuacc augacgaguu cccaacuaua uaucaccucc      540

gcaaauaccu ggcugauucg acaaagaaag cagaccugcg acucguguac uuagcccugg      600

cucauaugau caaguauaga ggacacuuuc uuauugaggg cgaguucaac ucaaagaaua      660

acgauaucca gaagaauuuu caggacuucu uggacaccua caacgccauu uuugaaagcg      720

aucugagucu agaaaauucc aagcagcucg aagagaucgu caaagacaag auaucuaaac      780

uggagaagaa agauaggauu cuuaagcugu uccccggcga aaagaacagc gggaucuuuu      840

ccgaguucuu gaaacucauu gugggaaauc aagcggacuu ccggaagugc uuuaaccugg      900

augaaaaagc aucauuacau uucaguaagg agagcuauga cgaagaucug gagacgcucc      960

uugguuacau cggcgacgau uauucugacg uauuucugaa agccaagaag uuguacgaug     1020

cuauccuacu guccggguuc cucacuguua ccgacaauga gacagaagcc ccucugucaa     1080

gcgcaaugau uaaacguuau aacgagcaca aggaagaccu ugcucuguug aaagaguaca     1140

uacgcaacau cagucucaag acuuacaaug aaguguuuaa agaugacacc aagaacggau     1200

augccggcua cauugauggu aagacaaauc aggaggacuu cuaugucuac cugaaaaagu     1260

uacuggcgga guuugaaggg gccgauuauu uccuagagaa aaucgacaga gaagauuuuc     1320

ucaggaagca gcggaccuuc gacaacggau cuauaccaua ccagauucac cuucaagaga     1380

ugcgagcaau ccuugauaaa caggcuaagu uuuaucccuu cuuggccaaa aauaaggaac     1440

gcaucgagaa gauucugacg uuuagaaucc cuuacuacgu gggcccacuc gcuaggggca     1500

acuccgacuu cgccuggagc auucggaaac gcaaugagaa gauaacuccg uggaacuucg     1560

aagaugugau cgacaaagag ucgucugcag aagccuuuau uaauagaaug acauccuucg     1620

aucuguaucu ucccgaggag aagguccugc cuaaacauuc auugcucuac gaaaccuuua     1680

acguuuauaa ugagcugacu aaggugaggu ucaucgcuga aaguaugcgu gacuaccagu     1740

uuuuagacag caagcagaaa aaggauaucg uacggcugua uuucaaagac aagcgaaaag     1800

ugacagauaa ggacauuaua gaguaccuac acgcgaucua uggguacgau ggaauugaac     1860

ucaaggguau cgagaaacaa uuuaacuccu cucugagcac cuaccaugac cuuuugaaua     1920

uuaucaacga uaaggaguuc cuggacgauu caaguaauga agcaauaauc gaggaaauua     1980

uccacacgcu caccauuuuu gaggacagag aaaugaucaa acagaggcug uccaaguucg     2040

agaacauauu ugauaaaagc gucuuaaaga agcugucucg ccggcacuau acaggcuggg     2100

ggaaacuuuc agccaaguug auuaacggaa ucagagacga gaaaaguggc aauacuauuc     2160

ucgauuaccu gaucgacgac gguauaagca acaggaauuu caugcagcua auccaugaug     2220

acgcucuguc cuuuaagaaa aagauucaaa aagcccagau cauuggggau gaagacaagg     2280

gcaacaucaa ggaggugguu aaaucucucc caggaucgcc cgcaauaaag aaaggcauuc     2340

ugcagagcau caagaucgug gaugaacuug ucaaagugau gggagggcgc aagccugagu     2400

ccauuguagu ugaaauggcc cgagagaauc aguauaccaa ccaagguaag ucaaauaguc     2460

agcagcgguu gaaacgucug gagaagucuu uaaaagaacu cggcagcaag auccugaaag     2520

agaacauucc agcuaagcug uccaagauag acaauaacgc ccugcagaau gauagauugu     2580

accucuauua ccugcaaaac gggaaagaca uguauacagg agaugaccua gauaucgaca     2640

ggcugucaaa uuacgacauu gaucacauca uuccccaggc auuccucaag gacaacagca     2700

ucgauaauaa agugcugguc ucuuccgcga guaaccgcgg caagagcgac gaugugccgu     2760

cucuugaagu cgugaaaaag cggaagacuu ucugguacca guugcugaaa uccaaguuaa     2820

uaucacagag aaaauuugac aaccucacca aggcugagag gggugggcug aguccugagg     2880

auaaagccgg cuucauccaa cgacagcuug uugaaacacg ccagauuacc aagcaugugg     2940

cacggcugcu agacgagaag uuuaauaaca aaaaggauga aaauaacaga gccguaagga     3000

cugucaaaau cauuacgcuc aagagcacac uggugucgca guuccguaaa gacuuugagu     3060

uguauaaggu ucgcgaaauc aaugauuucc accaugcuca cgacgccuac cugaacgcag     3120

uggucgcuuc cgccuuacuc aaaaaguauc ccaagcugga gccagaguuu guguacggag     3180

auuauccuaa auacaauucu uuccgggaaa gaaagagcgc gaccgagaaa guauauuuuu     3240

acucaaacau aaugaauauu uucaagaaaa guaucucccu ugccgacggc agggugaucg     3300

aacgaccccu gauugagguu aacgaagaga cuggggaguc ugucuggaau aaggaaagcg     3360

acuuggcuac cgugcggcgc gugcucuccu acccacaagu caacguugug aagaaagugg     3420

aggaacagaa ucacggacug gauagaggua agcccaaagg ccuauuuaac gcaaaucugu     3480

caagcaagcc uaaaccaaac aguaacgaga aucuuguagg ggccaaggaa uaucucgacc     3540

cgaagaaaua cggaggcuau gcugguauau cuaacuccuu cacaguccug gugaagggga     3600

ccaucgagaa aggcgccaag aaaaagauua cuaauguuuu ggaguuccag ggaaucagca     3660

uucuggauag gaucaacuac cggaaggaca aauuaaauuu ucuccuggaa aagggcuaua     3720

aagauauaga gcuuaucauu gaacugccca aguacucguu guucgagcuc ucagacgggu     3780

cucgaagaau gcuggcaagu auccuaucca caaacaauaa acgcggagag auucauaagg     3840

gcaaccaaau cuuucugagc cagaaauucg ugaaacuucu cuaucacgcg aagaggauau     3900

cuaauacgau uaacgaaaau caucguaaau acgucgagaa ccacaagaaa gaauuugagg     3960

aacuguucua cuauaucuug gaguuuaaug agaacuacgu gggugccaag aaaaauggga     4020

aguuacugaa cagcgcuuuc caguccuggc agaaucacuc aaucgaugaa cucuguagua     4080

gcuuuauugg accuaccggc ucugagcgga agggucuguu cgaacuuacu uccagaggcu     4140

cagccgcaga cuuugaguuc cuggggguua aaaucccaag guaucgcgau uacacaccca     4200

guagcuugcu aaaggacgcu acccugauac aucaauccgu gacaggacuc uaugaaacuc     4260

gaauugaucu ggccaaacuu ggcgagggga agagaccugc agccaccaaa aaggcuggac     4320

aggcgaagaa aaagaaauaa ggacuaguga agggaacacc caaauuuaau ucagccuuaa     4380

gcacaauuaa uuaagaguga aacguaauug uacaagcagu uggucaccca ccauagggca     4440

ugaucaacac cgcaaccuuu ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu     4500

cagcuugcuu agauguucca aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa     4560

aaaaaggcca caaaaguucc cucucacuuu caguaaauaa aauaaaagca gcaacagaaa     4620

uaaagaaaua aaugaaauuc aaaaugaaau aaauauugug uugugcagca uuaaaaaauc     4680

aauaaaaauu aaaaaugagc aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               4776


<210>  3496
<211>  3858
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3496
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc cgcguucaag ccaaacagca uuaauuacau acugggacuc gacaucggua      180

uugcuuccgu uggcugggcc augguggaga ucgaugaaga ggaaaacccg auuaggcuua      240

ucgaccuggg gguacgggug uuugagcgcg cagaaguccc caaaaccgga gauucuuugg      300

ccauggcucg acguuuagca agaucaguga ggcggcugac acgcagaagg gcccaucggc      360

uacuccgcac ucgaagacug cuuaagaggg agggcguucu gcaggcugcc aauuucgacg      420

agaacgguuu gauaaagagu cucccuaaua cgccauggca acugcgggcg gcagcccugg      480

aucguaaacu cacccccuua gaauggagcg cugugcugcu ucacuugauc aagcaucgcg      540

gguaucuguc ccagaggaaa aacgagggag aaacagccga caaggagcua ggcgcacucc      600

ugaaaggcgu cgcugggaau gcccacgcgc uucagacugg agauuuuagg accccugccg      660

agcuggcauu gaacaaguuc gaaaaggagu cuggucauau ucggaaucag cgaucggacu      720

acucacacac auuuagccgc aaagaucucc aagcugaacu gaucuuacug uucgagaagc      780

agaaagaauu uggcaaccca cacgugagug ggggacucaa ggagggcauu gagacccuuc      840

ugaugacuca gagacccgcc uuguccggug acgcuguaca gaaaaugcua gggcauugca      900

cauucgaacc ugccgagcca aaggcagcca aaaauaccua uacggcugaa agguuuaucu      960

ggcugacuaa gcucaacaau cugcggauac uugagcaagg aucugaacgc ccgcugaccg     1020

auacagagag agcgacuuug auggacgagc ccuacagaaa gagcaaacuc accuaugcac     1080

aggcccguaa gcuguuaggc cuggaagaua cagcuuucuu uaaaggccua cgguacggga     1140

aggacaacgc cgaggcaucc acccucaugg aaaugaaagc cuaucacgcu auuucacgag     1200

cccuggagaa ggaaggacuu aaggacaaaa agaguccuuu gaaucugagc ccagagcucc     1260

aggaugagau cgguacggca uucucucugu uuaaaacuga cgaagauauu acaggcagac     1320

uuaaggacag gauccagccc gagauccugg aagcguugcu caaacauauu uccuucgaua     1380

aguucguuca aauaucacug aaggcuuuac gccggaucgu gccucugaug gagcagggga     1440

aaagauacga cgaggccugu gcagaaauuu acggagauca cuauggcaag aaaaacaccg     1500

aggaaaagau cuaccuacca cccauaccgg ccgacgagau uaggaacccu gucgugcucc     1560

gcgcucugag ccaggcccga aaagugauca auggugucgu ucggcguuau gggagucccg     1620

caagaaucca cauugaaacu gcuagggagg ugggcaaguc uuuuaaggau cgcaaagaga     1680

ucgaaaagcg gcaagaggaa aacagaaaag acagggagaa ggccgcggcc aaauuccgag     1740

aauacuuucc aaauuucgua ggagagccua aguccaaaga uauucuuaag uugcgccugu     1800

augagcagca gcauggcaag ugccucuaca gcgggaaaga aauaaaccug ggacgguuaa     1860

augagaaggg uuauguggaa aucgaccacg cucugcccuu uucgagaaca ugggacgauu     1920

cuuucaacaa uaaaguccuu guguugggcu ccgagaacca gaauaagggg aaccaaaccc     1980

cauacgaaua cuuuaaugga aaagacaacu caagggagug gcaggaguuc aaggcacgug     2040

uugaaacgag ucgcuuuccc cggagcaaga aacagaggau ucuccugcag aaguucgaug     2100

aggacggcuu uaaagaaaga aaucuaaacg auacccgaua ugugaaucgg uuccuguguc     2160

aauuugucgc cgaccgcaug agacucacag guaaggggaa aaagagggug uucgcuucca     2220

acggccagau cacuaaccug cuucggggau ucuggggcuu gcgaaaggua agagccgaga     2280

augaucgcca ucacgcacug gacgcgguug uggucgccug cucuaccgug gcuaugcagc     2340

agaaaaucac aagguuuguc cguuacaagg agaugaacgc cuucgauggg aaaacuauug     2400

acaaggaaac cggagaggug uuacaucaga aaacacacuu uccucagcca ugggaguucu     2460

uugcacagga aguuaugaua cggguguucg guaagccgga uggcaagccc gaauuugagg     2520

aggcugacac ccucgaaaaa cugagaacuc uucuggccga gaaguugagc ucaaggccug     2580

aagcaguaca cgaguauguc acgccacucu ucgugagucg cgcccccaau cgaaaaaugu     2640

ccgggcaggg acauauggaa acaguuaaga gcgcuaaaag acuggaugag ggcgugucug     2700

uccuacgggu gccucugacc caacucaagc ugaaagaccu ugagaagaug guaaacaggg     2760

aacgcgagcc aaaguuguac gaagcgcuga aagcccguuu agaggcacac aaggacgauc     2820

ccgcuaaagc cuuugccgaa ccguucuaua aguacgacaa agcugguaau agaacucaac     2880

aggugaaggc aguucggguc gagcaagugc agaagaccgg gguguggguc aggaaccaua     2940

auggcaucgc cgauaacgcg acaaugguuc gcguggacgu guuugagaaa ggagauaagu     3000

auuaccucgu accuauuuac ucauggcagg ucgcuaaagg cauccugcca gaccgagccg     3060

ugguucaggg gaaggaugaa gaggacuggc aacuuauuga ugacaguuuc aauuuuaaau     3120

ucagccugca ccccaacgau cuaguggaag ucaucaccaa gaaggcaaga auguucggau     3180

auuuugccuc cugucaccgg ggcacuggua auauaaacau caggauucau gaccucgauc     3240

acaaaaucgg gaagaauggc auucuggagg gaaucggugu gaaaacagcu uugucuuucc     3300

agaaguacca gauagacgag cugggcaaag aaauucgccc uugcagauua aagaagaggc     3360

ccccaguucg gaaacguccu gcagccacga agaaagcggg gcaggcuaag aaaaagaagu     3420

aaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu aauuaagagu     3480

gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac accgcaaccu     3540

uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc uuagauguuc     3600

caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu     3660

cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau     3720

ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga     3780

gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaagaauu                                                   3858


<210>  3497
<211>  4614
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3497
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaaaccauac accauuggac uggacauagg uacaaacagc guuggcuggg      180

ccgugcucac ugaucaguau gaccuuguaa agaggaagau gaaaaucgcg ggggauuccg      240

agaagaaaca aauuaagaaa aauuucuggg gagugcggcu guuugacgaa ggccagacgg      300

cugccgaucg ccgaauggca cguaccgcca gaaggcggau cgagcgcaga aggaaccgga      360

uuucuuacuu gcaggguauc uucgcugaag agaugucaaa gacagacgca aacuuuuucu      420

gccgcuuaag ugauagcuuu uaugucgaca augaaaagcg aaauuccaga cauccguucu      480

uugccacuau agaggaggaa guggaguacc acaaaaacua ucccaccauc uaccaucuga      540

gggaggagcu aguuaauucu ucggaaaagg cugaucuccg gcugguguau cuugcccugg      600

cgcacauuau caaauaccgu gggaacuucu ugauugaagg agcacucgac acacagaaua      660

ccucagucga uggcaucuac aagcaauuua uacagacuua uaaccaggug uucgccagcg      720

gcauugagga cgggagucug aaaaagcugg aagauaauaa agacguagcu aagauccucg      780

uugagaaggu gacacgcaaa gaaaaguuag agaggauucu gaaacuuuac ccuggagaga      840

aguccgccgg uauguuugca caguucaucu cuuugaucgu cggcagcaaa gggaacuuuc      900

aaaagccauu cgaccugauu gaaaaguccg auauagagug ugcuaaagac ucauaugaag      960

aggaucuaga aagucuccug gcccuuaucg gagacgagua cgcggagcug uucguggccg     1020

caaagaaugc uuauagcgcc guggucuugu cuuccauuau caccguugcu gaaacggaga     1080

cuaacgccaa acucucagca agcaugauag aaagguuuga uacccaugag gaagaccugg     1140

gcgaguuaaa ggccuucauu aaacugcacc uccccaagca cuacgaggaa aucuuuagua     1200

auacagagaa gcaugguuau gcuggguaca ucgauggaaa aacuaagcag gcggacuucu     1260

acaaauauau gaagaugacc cuugaaaaca uugagggcgc agauuacuuu aucgccaaaa     1320

uugagaagga aaacuuccug cggaagcagc gaacauuuga caauggcgcu auaccucacc     1380

aguugcaucu agaggaacug gaggccaucc uccaccaaca ggcaaaauau uacccauucc     1440

ugaaggaaaa cuaugauaaa auuaagucuc uugugaccuu ucgcaucccc uacuucguag     1500

ggccucuggc caauggacag uccgaguuug cuugguugac gagaaaagcc gacggugaga     1560

ucaggccaug gaacauugaa gagaaggugg acuucggcaa aagcgcaguc gauuuuauag     1620

aaaagaugac uaauaaggac acauaucucc cgaaagagaa cgugcugccc aagcacucgu     1680

uaugcuacca aaaauaccug guuuauaaug aacuaaccaa ggugcgguac aucaacgauc     1740

aggggaaaac uucuuauuuc uccggacagg agaaggagca gauuuucaau gaccucuuua     1800

agcaaaaacg caaggucaaa aagaaagauc uggaacuuuu cuugagaaac augucacaug     1860

uggagagucc uacaaucgaa ggccuggagg acagcuuuaa uuccucuuac agcaccuauc     1920

acgaucuccu gaagguaggu auuaagcagg aaauccuuga caacccaguu aauacggaga     1980

ugcuggagaa cauagugaaa aucuugaccg ucuucgaaga uaagaggaug auuaaagagc     2040

agcuccagca auuuucagac gugcuggaug gggucguguu aaagaaacug gaacgucggc     2100

auuacacagg cuggggacga cuaagugcga agcuccugau gggcaucaga gacaagcagu     2160

cccaccuuac uauuuuggau uaucugauga augacgacgg gcucaacagg aaccugaugc     2220

aguuaaucaa ugauagcaac cugucuuuca aaucaauaau ugagaaggag cagguuacca     2280

cagcugacaa agauauccaa aguauugugg ccgaccuugc aggaagcccc gccaucaaga     2340

aagguauauu gcagucccuc aagaucguag augaacuggu cucugugaug ggcuacccuc     2400

cacagacuau uguuguggag auggcucgcg aaaaucagac cacagggaaa ggaaagaaca     2460

auucgcggcc cagauacaag agccuagaga aagccaucaa ggaauuuggc ucccaaauuc     2520

ugaaagagca uccgaccgac aaccaggagc ucaggaauaa ccgccuguau cuuuacuauu     2580

ugcagaaugg uaaggauaug uacacugggc aagaccugga uauccacaac uuaucaaauu     2640

augacauaga ucacauuguc ccucagaguu ucaucacgga caacucuauc gauaaucucg     2700

ugcugacaag cuccgcaggc aaccgagaaa aaggagacga cguaccccca cuugagauug     2760

ugcggaagcg uaaggucuuu ugggaaaaac uguaccaggg caauuugaug ucaaagagaa     2820

aauucgauua ucucaccaag gcugagagag ggggacugac ugaagccgac aaagcgcgcu     2880

uuauccaucg gcagcuaguu gagaccagac aaauuacaaa gaacguggcc aacauacugc     2940

accagagguu caauuacgag aaggaugacc augguaacac caugaaacag gugcgaaucg     3000

ucacucucaa gagcgcucug guuucucagu uucgcaaaca auuccagcuu uacaaggugc     3060

gggaugugaa ugacuaucac cacgcacaug augccuacuu gaacggcgua gucgcuaaua     3120

cacuguuaaa aguguauccu cagcucgaac ccgaguucgu uuacggggac uaucaccagu     3180

uugauugguu caaggccaac aaggcaacgg cgaaaaagca auuuuacacc aauauuaugc     3240

uguucuuugc ccagaaagac agaaucauug augaaaacgg agagauccuu ugggacaaga     3300

aauaucugga cacugugaag aaggucaugu ccuacaggca gaugaauaua gugaaaaaga     3360

cagaaaucca aaaaggcgag uucaguaagg cuaccauuaa accaaagggu aacagcucua     3420

aacuaauccc ccguaagaca aauugggauc cuaugaagua cgggggccuc gacuccccaa     3480

acauggccua ugcaguugug auugaguacg cuaaaggaaa gaauaaacug guauuugaaa     3540

agaaaaucau acgcgucacu auuauggagc ggaaggccuu cgaaaaggau gagaaagcau     3600

uuuuggagga acagggcuau agacagccga aggugcuggc caaauuaccc aaguacaccc     3660

ucuaugagug ugaagagggg aggcgacgga ugcuggcuuc agcgaacgaa gcccagaaag     3720

gaaaucaaca ggugcuuccu aaccaucugg ucacguugcu ccaccaugca gcuaacugcg     3780

agguuaguga cggcaagagc cuggauuaca ucgagucgaa ucgcgaaaug uucgccgagc     3840

uacuggccca cguguccgaa uuugcuaaga gauauacccu ugcagaggcc aaccucaaua     3900

aaaucaacca gcuguucgaa cagaauaagg agggugacau uaaagcgaua gcucaaucuu     3960

ucguagauuu gauggccuuu aacgcaaugg gggccccagc uagcuucaag uuuuucgaga     4020

caacuaucga aaggaaacgg uacaauaacc ugaaggaguu acucaauuca accauuaucu     4080

accagaguau uacaggacug uaugaauccc gaaagagacu ugacgauaaa cgccccgcag     4140

ccacuaagaa agcgggccag gcuaagaaaa agaaauaagg acuagugaag ggaacaccca     4200

aauuuaauuc agccuuaagc acaauuaauu aagagugaaa cguaauugua caagcaguug     4260

gucacccacc auagggcaug aucaacaccg caaccuuucc uuuuuccccc agugauucug     4320

aaaaaccccu cuucccuuca gcuugcuuag auguuccaaa uuuaguaagc uuaaggcggc     4380

cuacagaaga aaaagaaaaa aaaggccaca aaaguucccu cucacuuuca guaaauaaaa     4440

uaaaagcagc aacagaaaua aagaaauaaa ugaaauucaa aaugaaauaa auauuguguu     4500

gugcagcauu aaaaaaucaa uaaaaauuaa aaaugagcaa gaucuaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           4614


<210>  3498
<211>  4647
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3498
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaaaccauac agcauuggac uggacauagg uaccaacucc guuggcuggg      180

ccgugguaac agaugacuau aaggugccgg cgaagaaaau gaagguccuc gggaauacug      240

auaaaucuca uaucgagaag aaccuucugg gagcuuuguu auucgacuca ggcaauacgg      300

ccgaagauag gcggcugaaa cgcaccgcac gacguagaua cacaaggcgg cgcaaccgga      360

uucuauaucu ccaggagauc uuuagugaag agauggguaa gguggacgau agcuucuuuc      420

acaggcugga agacuccuuc cuuguuacug aggauaagag aggggagcgc caucccauuu      480

uuggaaaucu ggaagaggaa gugaaauacc acgagaacuu cccuaccauc uaucauuugc      540

gacaauaccu cgccgacaau ccagagaagg ucgaucugag acugguguau cucgcuuuag      600

cacacauaau caaauuuagg ggccacuucc ugauugaagg caaguuugac acacggaaca      660

augauguaca gcgucuuuuc caggaguuuu uggccguuua cgacaacacc uucgaaaauu      720

cuucgcugca ggagcaaaac gugcaggucg aagagauccu aacugacaaa auuucaaaga      780

gcgcuaaaaa ggaucgcgug cucaagcugu uucccaauga gaaaaguaac gggagauucg      840

ccgaguuccu uaagcugauc gugggaaauc aggcggacuu uaaaaagcau uucgaguugg      900

aagagaaagc accucuccag uuuuccaagg auacauacga agaggagcug gaagucuuac      960

uggcccaaau aggugacaac uaugcugaac ucuuccuuuc ugccaagaaa cuguacgaua     1020

gcauuuugcu auccggcauc cugaccguua cggacguggg gacuaaggca ccacucucag     1080

cuaguaugau ucagagguau aacgaacacc agauggaucu ggcccagcuu aaacaauuua     1140

uccggcagaa gcugagcgac aaauacaaug agguauucuc ugaugugucc aaggacggau     1200

augcgggcua caucgauggu aagaccaacc aggaagccuu uuauaaauac uugaaggggc     1260

uccugaauaa aauugaggga ucaggcuacu ucuuagacaa gauagagcga gaagacuuuc     1320

ugcgcaaaca aagaacauuc gauaacggca gcauccccca ucagauucac cuacaggaga     1380

ugagggcaau cauacggcgc caggcugaau uuuauccuuu ccucgccgac aaucaagaua     1440

gaauugagaa gcugcuuacu uuuaggaucc cauacuaugu cgggccguug gcucguggaa     1500

agagugacuu cgccuggcug ucucggaaau ccgcagauaa gaucaccccc uggaacuucg     1560

acgagauugu ggauaaagaa agcucggccg aggcuuuuau caaucgaaug acaaacuacg     1620

accucuaucu gccuaaucag aagguucuuc caaaacacuc ucuguuguac gaaaaguuca     1680

ccguguauaa cgagcucacg aaagucaagu acaagacuga acaggguaaa acagcguuuu     1740

ucgaugcaaa uaugaagcag gagauuuuug acggcguguu caaaguauac agaaagguua     1800

ccaaagauaa gcugauggac uuuuuagaga aggaguucga ugaauuuagg auaguggacc     1860

ugacugggcu agacaaagaa aacaaggucu ucaaugccuc cuauggaaca uaccaugauc     1920

ucugcaaaau ccuggacaag gauuuucuug acaacaguaa aaaugagaag auuuuggaag     1980

auaucgugcu gacccucacg cuguucgagg accgcgagau gauccggaag agauuagaaa     2040

acuauucaga ucugcuuacc aaagagcaag ucaagaaauu ggaaaggcgc cacuacacag     2100

gcuggggucg acucagcgcu gagcugauuc augggauacg gaacaaggaa ucccguaaaa     2160

cuauccuaga cuaucugauu gaugacggca auucuaacag aaauuuuaug cagcucauca     2220

acgaugacgc ccugagcuuc aaggaggaga uugcaaaggc ccaggugauc ggagaaaccg     2280

auaaucuuaa ccagguugug ucagacauag cuggcagucc cgccaucaaa aaggggauuu     2340

ugcaaucccu gaaaaucgua gacgaguuag ucaagauuau gggacaccag ccugaaaaua     2400

ucgugguuga gauggcaagg gagaaccagu ucacaaauca gggucgccgg aacagccaac     2460

agagacucaa aggccugacu gauucuauaa aggaauuugg gucacagauu cuuaaagagc     2520

auccagugga aaauagucaa cugcagaacg acagguuguu ccucuacuau cugcagaaug     2580

gacgagauau guacaccggc gaggaacuag acaucgauua ccugagccag uaugacauug     2640

aucacaucau accccaagcg uuuaucaagg acaacuccau ugauaaucgc guccucacau     2700

cuucgaagga gaaccggggu aaaagcgacg augugccguc caaggacgua gugagaaaaa     2760

ugaagucaua cuggaguaaa cugcuuucug cuaaguugau cacccagagg aaguucgaca     2820

accugacuaa agccgagcgu gggggcuuaa cggaugacga uaaggcagga uuuauuaaac     2880

gccagcucgu ugaaacacgg cagaucacca agcacgucgc cagaauacug gacgagaggu     2940

ucaauacuga aaccgaugag aacaauaaaa agauucgaca agugaagauc gugacacuua     3000

aaagcaaccu ggucuccaau uuucggaagg aguucgaacu auauaaaguu cgcgagauca     3060

acgacuacca ucacgcucau gaugccuauc ucaaugcagu gauuggcaag gcucuguugg     3120

ggguguaccc ucagcuggaa cccgaguuug uauauggaga cuacccacac uuccacgguc     3180

auaaagaaaa caaggccacc gcgaagaaau uuuucuacuc aaauaucaug aacuucuuua     3240

agaaagauga cgucagaacu gauaagaaug gcgagauuau auggaaaaag gacgaacaca     3300

ucagcaacau uaagaaagug uuaucuuauc cucagguuaa uaucgugaag aaagucgagg     3360

agcagacagg gggauucucc aaggaaagua uucuccccaa aggcaacagc gauaagcuga     3420

ucccaaggaa aacgaagaag uuuuacuggg acaccaaaaa guaugguggg uucgacucuc     3480

ccauaguggc cuacuccauc cuuguuauug cugauaucga gaaaggcaag aguaaaaagc     3540

ugaagacugu gaaagcauug guaggaguca caauuaugga aaagaugacc uuugagcggg     3600

acccuguggc cuuccucgag cgaaaaggcu auagaaaugu gcaagaagag aacaucauaa     3660

agcugccaaa auacucacua uuuaagcugg aaaacgggcg caagaggcuu cucgcuagcg     3720

cccgugagcu gcagaaagga aaugaaauug ucuugccgaa ccaucugggc acauuacucu     3780

aucacgcaaa gaauauccau aaaguugaug agcccaagca ccuggacuac guggauaaac     3840

acaaggacga guucaaggaa cuucuggaug uagugucgaa cuuuuccaaa aaguacacuu     3900

uggcggaggg uaaucucgag aaaaucaagg aacuguaugc ccagaacaau ggggaagacc     3960

uaaaagagcu ggcuucuagc uucauuaacc uucucaccuu uacggccaua ggagcuccug     4020

caaccuucaa guuuuucgau aaaaauaucg accggaagag auacacauca acuaccgaga     4080

uucugaacgc cacauugauc caucaaagua uuacuggcuu auaugaaacc aggaucgauc     4140

ugaauaagcu cgguggcgac aaacgcccag cagccacgaa gaaagcuggg caggcgaaga     4200

aaaagaagua aggacuagug aagggaacac ccaaauuuaa uucagccuua agcacaauua     4260

auuaagagug aaacguaauu guacaagcag uuggucaccc accauagggc augaucaaca     4320

ccgcaaccuu uccuuuuucc cccagugauu cugaaaaacc ccucuucccu ucagcuugcu     4380

uagauguucc aaauuuagua agcuuaaggc ggccuacaga agaaaaagaa aaaaaaggcc     4440

acaaaaguuc ccucucacuu ucaguaaaua aaauaaaagc agcaacagaa auaaagaaau     4500

aaaugaaauu caaaaugaaa uaaauauugu guugugcagc auuaaaaaau caauaaaaau     4560

uaaaaaugag caagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaaaaaa aagaauu                                         4647


<210>  3499
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3499
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaca gacaacaaac cugagcuaca uucucggacu ugaccugggu auagccuccg      180

uuggcugggc ggugguagag aucaaugaaa acgaggaucc aauuggguug aucgacgugg      240

gagucaggau uuucgaacgg gcugaggugc cgaagacugg cgaaucuuua gcccugucac      300

gccgacuagc acguaguacg agaaggcuca uccggcgcag agcccauagg cugcuucugg      360

cuaaacgguu uuugaagcgc gaggguauac ucagcaccau cgaucuggag aaggggcugc      420

ccaaucaagc augggaacuc cgaguugccg gauuagagag aaggcugucc gcuauugaau      480

ggggcgccgu gcuuuugcac cugaucaaac aucggggcua ucuaucuaag cguaaaaacg      540

agucgcagac aaauaacaag gagcucgggg cgcugcuuuc aggagucgca cagaaucacc      600

agcuguugca aagcgacgau uaccgcacuc cugccgaacu cgcucugaaa aaguucgcca      660

aggaggaagg ucauauuaga aaccagaggg gcgcauauac ccacacauuu aaucgguuag      720

accugcucgc ugagcuuaac cuguuguucg cccagcagca ccaauuuggg aauccacauu      780

gcaaagaaca cauccagcag uacaugaccg agcuacugau guggcagaag cccgcgcuca      840

guggagaggc cauacugaaa augcuuggca aguguacuca ugagaaaaac gaguucaagg      900

cagcuaaaca cacauauucc gccgaacgau uuguguggcu gaccaaguug aauaaccucc      960

gcauucugga ggauggugcu gaaagagccu uaaaugagga ggaaaggcaa cugcuaauca     1020

accacccuua cgagaagucu aaacucacgu augcacaggu acggaagcug cuuggguuga     1080

gcgaacaggc cauuuucaaa caucugcgcu acuccaagga gaacgcugaa ucagcgacuu     1140

uuauggagcu caaagcaugg cacgccauca gaaaggcucu ggagaaucaa ggacuuaagg     1200

acaccuggca ggaucuggcc aaaaagccag acuugcucga ugaaaucggc acagcauuca     1260

gucuguacaa aacugacgag gauauucagc aguauuuaac caacaagguu cccaauagcg     1320

ugauaaacgc ccugcuaguc ucucucaauu uugacaaauu caucgaacug ucccuuaagu     1380

cauugaggaa gauucugccu cucauggagc aaggcaaacg uuacgaccag gcuugccggg     1440

agaucuaugg gcaucacuac ggagaagcca accagaagac aagccagcug uuaccagcaa     1500

uaccggcgca agagauucga aaucccgugg ugcugagaac ccuuagucag gcuaggaaag     1560

ucaucaacgc caucauucgc caguaugguu cuccugcacg gguucauauc gaaacgggca     1620

gagaguuggg gaaguccuuu aaagaaaggc gcgagauuca gaagcaacag gaggauaauc     1680

gaacuaagcg ggaaagcgcc gugcagaaau ucaaggaacu cuucucggac uuuucuuccg     1740

aaccaaaauc aaaggauaua cugaaauucc gucuauacga gcagcaacac ggaaaguguc     1800

uguauagugg caaagaaauc aacauucaca gacucaauga gaaggguuac guagagaucg     1860

accaugcucu gcccuuuagc aggacauggg augacuccuu caacaauaag gugcuugucu     1920

uggccucuga aaaccagaau aaagggaacc agaccccuua cgaguggcug caaggcaaga     1980

ucaacagcga acgcuggaaa aauuuugugg cauuaguucu cggaucacag ugcagugcug     2040

ccaagaaaca gcggcugcuu acucagguga uugaugacaa caaguucaua gauagaaauc     2100

ugaacgacac aagguauauc gcgcgauuuu uguccaauua cauucaagag aaccuccugc     2160

uagucggcaa gaauaaaaag aacguguuca ccccaaaugg gcagaucacg gcccugcucc     2220

gcagccggug gggacugauu aaagcuagag aaaacaauaa caggcaccau gcacuugaug     2280

ccaucguagu ugcuugugcc acccccucua ugcagcagaa gauaacacgu uuuauccgcu     2340

ucaaagaggu gcacccguau aagauugaga aucgguacga aauggucgac caagagucag     2400

gugaaaucau uaguccucac uuucccgagc caugggcaua uuucagacag gaggugaaca     2460

ucagggucuu ugacaaucau ccugauacug uguugaagga aaugcugccc gaccgaccac     2520

aggcgaacca ccaguucguu caacccuuau ucgugagccg ggccccuacc cgcaaaaugu     2580

ccggccaggg gcauauggag acaauaaagu cugcuaaaag acucgccgaa ggaauuucgg     2640

uacugaggau cccacuuacu cagcugaagc cgaaccuacu cgagaauaug gucaacaaag     2700

aacgggagcc cgcacuguac gcuggcuuga aggcccgacu ggcagaguuu aaucaagauc     2760

cugccaaggc uuucgcgacc ccauuuuaua aacagggugg gcaacaggug aaggccauua     2820

gaguugaaca ggugcagaaa agcggcgucu uagugcgcga gaacaaugga guagcagaca     2880

acgcuuccau cgugaggaca gauguuuuca uaaagaauaa caaauuuuuc cucgucccca     2940

ucuacaccug gcaaguggcc aagggcauuc ugccuaauaa agccaucgug gcucacaaga     3000

acgaagacga gugggaagag auggaugagg gggcaaaguu uaaauucuca cuuuuuccaa     3060

augaccuggu cgaauugaag acuaaaaagg aguacuucuu uggauauuac auuggucucg     3120

aucgugccac gggcaacauc agucugaaag aacaugacgg ggagauaucu aagggaaagg     3180

auggcguuua ucgggugggu gugaaacuag cgcugagcuu cgaaaaguac cagguagacg     3240

agcuugggaa aaauagacag auuugcaggc cccagcagcg ccagccgguc cgaaagagac     3300

cugcugccac caaaaaggca ggccaagcca agaaaaagaa auaaggacua gugaagggaa     3360

cacccaaauu uaauucagcc uuaagcacaa uuaauuaaga gugaaacgua auuguacaag     3420

caguugguca cccaccauag ggcaugauca acaccgcaac cuuuccuuuu ucccccagug     3480

auucugaaaa accccucuuc ccuucagcuu gcuuagaugu uccaaauuua guaagcuuaa     3540

ggcggccuac agaagaaaaa gaaaaaaaag gccacaaaag uucccucuca cuuucaguaa     3600

auaaaauaaa agcagcaaca gaaauaaaga aauaaaugaa auucaaaaug aaauaaauau     3660

uguguugugc agcauuaaaa aaucaauaaa aauuaaaaau gagcaagauc uaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3780


<210>  3500
<211>  3858
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3500
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc cgcguucaag ccaaacccga uuaauuacau acugggacuc gacaucggua      180

uugcuagcgu uggcugggcc augguggaga ucgaugaaga cgagaacccc auuugccuua      240

ucgaucuggg gguaagggug uuugaacggg cagagguccc uaaaaccgga gacuccuugg      300

ccauggcucg ccgauuagca cguucuguga gaaggcugac acggcgcaga gcccauaggc      360

uacuccgggc ucgccgacug cuuaagagag aaggcguucu gcaggccgcg gauuucgacg      420

agaaugguuu gauaaaguca cucccaaaca cucccuggca acugagggca gccgcucugg      480

aucggaaacu cacgccuuua gaguggagug ccgugcugcu ucacuugauc aagcaucgug      540

gguaucugag ccagcgcaaa aaugaaggag agaccgcaga caaggaacua ggcgcucucc      600

ugaaaggcgu cgccgauaac gcgcacgccc uucagacagg ggacuuuaga acuccagcag      660

agcuggcuuu gaauaaguuc gagaaggaau ccggacauau uaggaaccag cggggugauu      720

acucucacac cuuuucgcga aaagaccucc aagccgagcu gaucuuacug uucgaaaagc      780

agaaagaguu uggcaauccc cacgugucag ggggacucaa ggaaggcauu gagacacuuc      840

ugaugaccca gcgcccugcu uugagcggug augccguaca gaaaaugcua gggcauugua      900

cuuucgagcc agcagaaccg aaggccgcua aaaacacaua uaccgcggag agauuuaucu      960

ggcugacgaa gcucaauaac cugaggauac uugaacaagg aagugagcgg ccccugacug     1020

acaccgaacg cgcaacauug auggacgagc cuuacagaaa guccaaacuc acuuaugccc     1080

aggcuaggaa gcuguuaggc cuggaggaua ccgccuucuu uaaaggccua cguuacggga     1140

aggacaaugc agaagccucu acacucaugg agaugaaggc uuaucacgcc auuagccggg     1200

cacuggaaaa ggagggacuu aaggauaaaa aguccccauu gaaccuguca cccgaacucc     1260

aggacgagau cgguaccgcg uucagucugu uuaaaacgga ugaggacauu acuggccgac     1320

uuaaggauag aauccagccu gaaauccugg aggcuuugcu caaacauauu agcuucgaca     1380

aguucguuca aauaucucug aaggccuuaa ggcgcaucgu gccacugaug gaacagggga     1440

aacgguacga ugaggcaugc gccgagauuu acggagacca cuauggcaag aaaaauacag     1500

aagagaagau cuaccuaccc ccgauaccug cugaugaaau uagaaacccc gucgugcuca     1560

gggcccuguc ccaggcacgc aaagugauca acggugucgu ucgacgguau gggucaccag     1620

cucguaucca cauugagacc gccagagaag ugggcaagag cuuuaaggac aggaaagaga     1680

ucgagaagcg ccaagaagag aaucggaaag acagagaaaa ggcggccgcu aaauucaggg     1740

aguacuuucc uaacuucgua ggagaaccca agaguaaaga uauucuuaag uugcgacugu     1800

augagcagca gcauggcaag ugucucuacu cugggaaaga gauaaaucug ggacgcuuaa     1860

acgaaaaggg uuauguggag aucgaccacg cacugccauu uucccggacu ugggaugaca     1920

gcuucaauaa caaaguccuu guguugggcu cggaaaauca gaacaagggg aaucaaacac     1980

ccuacgagua cuuuaacgga aaagauaauu cuagagaaug gcaggaguuc aaggccaggg     2040

uugagaccuc ccguuuuccu cgcucaaaga aacagcggau ucuccugcag aaguucgacg     2100

aagauggcuu uaaagagaga aaccuaaaug acacgaggua ugugaaccga uuccugugcc     2160

aauuugucgc ugaucggaug cgccucaccg guaaggggaa aaagagagug uucgccagua     2220

auggccagau cacaaaccug cuuaggggau ucuggggcuu gcggaaggua cgagcagaaa     2280

acgacagaca ucacgcgcug gaugccguug uggucgcuug uagcacugug gccaugcagc     2340

agaaaaucac ccgcuuuguc agguacaagg agaugaaugc auucgacggg aaaacaauug     2400

auaaggagac uggagaagug uuacaucaga aaacccacuu uccacagccg ugggaguucu     2460

uugcucagga aguuaugaua cguguguucg guaagcccga cggcaagccu gaguuugaag     2520

aggccgacac accagagaaa cuccggaccc ugcuugcaga aaagcugucc ucuagacccg     2580

aggccguaca cgaauauguc acuccuuugu ucgugagcag ggcuccaaac cgcaaaaugu     2640

cagggcaggg acauauggag acgguuaaga gugcgaaacg acucgaugaa ggcguguccg     2700

uccugagagu gccccuaaca caacugaagc ucaaagaccu ggagaagaug guaaaucggg     2760

agagggaacc gaaacuuuac gaggccuuga aagcacgccu ggaagcucac aaggaugacc     2820

cugccaaagc cuuugcugag ccauucuaua aguacgauaa agcagguaac cguacccaac     2880

aggugaaggc cguuagaguc gaacaagugc agaagacugg gguguggguc cggaaucaua     2940

acggcaucgc ggacaaugcu accaugguua ggguggaugu guuugagaaa ggagacaagu     3000

auuacuuagu acccauuuac agcuggcagg ucgccaaagg cauccucccu gaucgcgcag     3060

ugguucaggg gaaggacgag gaagauuggc aacugauuga cgauucuuuc aacuuuaaau     3120

ucucacuuca ccccaaugac cugguggagg ucaucacaaa gaaggcccga auguucggau     3180

auuuugcuag uugccacaga ggcaccggua acauaaauau ccggauucau gaccuagauc     3240

acaaaaucgg gaagaacggc auucucgaag gaaucggugu gaaaacugca cugagcuucc     3300

agaaguacca gauagacgag uugggcaaag agauuaggcc augucgccug aagaagagac     3360

cucccguuag gaaacggcca gccgcgacaa agaaagcugg gcaggccaag aaaaagaagu     3420

aaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu aauuaagagu     3480

gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac accgcaaccu     3540

uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc uuagauguuc     3600

caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu     3660

cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau     3720

ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga     3780

gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaagaauu                                                   3858


<210>  3501
<211>  3864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3501
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcacccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa guaccacguu ggaauugacg uggguaccuu cagcguaggc cuggccgcga      180

uagaggugga ugacgcuggg augccaauca aaacacucuc ccuugucucu cacauucaug      240

auucaggacu ggacccggau gaaaucaaga gugccgugac uagguuggca agcuccggca      300

uugcccggcg cacgcgacgu uuauauagaa ggaagcggcg cagacugcag caacuagaca      360

aauuuaucca gaggcagggu uggcccguua uagagcucga agauuacucu gacccucugu      420

auccauggaa ggugcgggcu gagcuugcag ccucguacau cgcugaugaa aaagagcgcg      480

gggagaagcu gucagucgcc uugcgacaca uugcgagaca uaggggaugg cggaaccccu      540

augcaaaagu gagcagucuc uaccugccug acggcccauc cgaugccuuc aaggcuaucc      600

gugaagagau uaagcgcgcc ucuggccagc ccguaccuga aaccgcaaca guugggcaaa      660

uggugacucu gugcgagcuc ggaaccuuaa aacugagagg ugagggcggg guccuuagcg      720

cuagguugca gcaguccgac uaugcccggg aaauccagga gauaugucga augcaagaaa      780

uuggacagga gcuguaccgc aagaucauug augugguguu ugcggccgaa ucaccaaaag      840

gcagugcaag cucuagaguc gguaaggacc cgcuacagcc cgggaaaaau agggcucuca      900

aggccuccga cgcuuuccag cgguaccgca ucgccgcacu gaucggaaac cuuagaguua      960

ggguggaugg cgagaaacgu auucugucag uagaggaaaa gaauuuggug uuugaccacc     1020

ucgucaaccu gacaccuaag aaagagccag aaugggugac cauagccgag aucuuaggca     1080

uugaucgggg gcaacugauc ggaacugcua caaugaccga cgauggugaa cgagcgggcg     1140

caagaccccc uacgcacgac acuaauagga gcauaguuaa cagucgcauu gccccacucg     1200

uggauuggug gaagaccgcu ucugcccuug agcagcaugc aauggucaaa gcccugucca     1260

augcugaggu ggacgauuuc gacagccccg aaggggccaa gguacaggca uuuuucgcgg     1320

auuuggacga cgauguucac gcuaaacuag acucgcugca ucucccggug ggacgggccg     1380

cauauucuga ggauacacug gucagacuua cuaggcgcau gcuguccgac ggcguggauu     1440

uguacaccgc ccgacuccaa gaauuuggua ucgagccuuc auggacaccc ccaaccccuc     1500

ggaucgggga acccgucggc aacccagcug uggaccgugu ucugaagacg gugaguagau     1560

gguuagagag cgccacuaag acauggggag cacccgagag gguaauuauc gaacacgucc     1620

gcgagggcuu cgugaccgaa aaacgggcua gagagaugga uggggacaug aggcgacgcg     1680

ccgcgcggaa ugccaagcug uuucaggaga ugcaggaaaa acuaaacguu cagggaaagc     1740

cuuccagagc ugaucucugg agguaucaau cugugcagcg ucagaauugc cagugugcau     1800

acugcgguag cccaauuacu uucucaaaca gugagaugga ccacauaguc ccgcgcgccg     1860

gccaaggguc cacaaauacc cgggaaaacc ugguggcugu augucauaga ugcaaucaga     1920

gcaaaggaaa cacgcccuuu gccaucuggg caaagaacac cucuauugag ggcgugucag     1980

uuaaggaagc ggucgagagg acacgacacu gggugacuga uaccgguaug cggaguacag     2040

acuucaaaaa guucacuaaa gccguggucg agcgcuuuca gagagcuacc auggaugaag     2100

agaucgacgc caggagcaug gaauccguug cauggauggc uaaugagcuu cggucucgag     2160

uggcccagca uuucgcaucg cacgggacaa ccgugagagu auaucgcggc agcuugacug     2220

ccgaagcuag gcgugcgucc ggaaucucag gcaagcugaa auuuuucgac ggggucggaa     2280

agagucggcu cgauagaagg caucacgcca uugacgcagc ugugauagcc uuuacgucug     2340

auuacguugc cgagacacug gcugugcgca gcaaccuuaa gcaaucccag gcacaccgac     2400

aggaggcccc ucaguggaga gaguucaccg guaaagacgc ggaacaucgg gcugccugga     2460

gggucuggug ucaaaagaug gaaaaacugu cagcauugcu cacugaggau cugcgcgacg     2520

aucguguggu ugugaugagc aauguaagau uacggcuggg caacgggucu gcccacaagg     2580

aaaccaucgg aaaacuaucc aaggucaaac ucaguagcca gcugucugug uccgacauug     2640

auaaggcuuc aagugaggca cuuuggugcg ccuugacaag ggagccaggc uuugacccca     2700

aggaaggucu gccugcgaau ccagagcgcc auauccgagu gaacgggacc cacgucuaug     2760

cuggcgauaa uauuggacuc uuccccguua gcgccggcuc gaucgcacug agagggggau     2820

acgccgaauu aggcuccucu uuucaccaug cucgggugua caaaauaacu agcgguaaga     2880

aaccggccuu cgcaaugcug aggguauaua caaucgaccu uuugccuuac cgcaaccagg     2940

aucucuuuuc aguggagcug aagccacaaa cgaugaguau gagacaggcu gagaaaaagc     3000

uaagggacgc ccuggcgacc gggaaugccg aauaucucgg auggcugguc guggacgaug     3060

agcuuguugu ggacacuucc aagauugcaa cagaucaggu caaagcugug gaagccgagu     3120

ugggcaccau ccggcguugg cgcguugacg guuucuuuuc ucccagcaag cugcgauuaa     3180

gaccucucca gauguccaaa gaaggcauua agaaagaguc agcucccgag cugagcaaga     3240

ucauagauag gccagggugg cuuccugcag ugaacaagcu guucagugac ggaaauguca     3300

caguagugcg gcgcgauucu uugggcagag uuaggcucga auccacugcc caucugcccg     3360

ugaccuggaa aguccaaaag cggccagccg cuacgaaaaa ggcggggcag gccaaaaaga     3420

agaaauaagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc acaauuaauu     3480

aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug aucaacaccg     3540

caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca gcuugcuuag     3600

auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa aaaggccaca     3660

aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua aagaaauaaa     3720

ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa uaaaaauuaa     3780

aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaag aauu                                            3864


<210>  3502
<211>  3564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3502
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc caggauucug gcguucgaca uaggaaucag cuccauuggu ugggcuuuuu      180

cugagaacga ugaacucaag gacugcggcg uucggaucuu caccaaagug gagaauccaa      240

agacagggga aucacuugcc cugccgcgcc gauuggcacg uagugccaga aagagguuag      300

cucggcgcaa agcaagacug aaccaucuaa agcaccucau ugccaaugag uuuaaacuga      360

acuacgaaga uuaucagagc uucgacgagu cccuugcuaa ggccuacaaa ggaucucuga      420

ucucgcccua ugaguugagg uuucgggcgc ucaaugaacu gcugucaaag caagauuucg      480

cacgcguaau acuccauauc gccaagcgaa gaggcuacga cgauauuaaa aacagcgacg      540

auaaggagaa aggugcuauc uuaaaggcca uuaaacagaa ugaagagaag cuggcaaacu      600

aucagagugu gggggaguac cuuuauaaag aauacuuuca gaaguucaag gaaaauucca      660

aagaauuuac uaacgucagg aauaagaaag agucuuacga acgguguauc gcucagagcu      720

ucuugaagga cgagcugaaa cuaauauuua agaagcaacg ugaguucgga uuuuccuucu      780

cuaaaaaguu cgaagaggaa gugcucagug uugccuuuua uaaacgcgcg cugaaggauu      840

ucagccaccu ugugggcaac ugcucauuuu ucacggacga gaaaagagcc ccuaaaaauu      900

ccccacuggc auuuauguuc gucgcuuuga ccaggauuau caaccuccug aauaacuuaa      960

agaacacaga aggcauucug uacacuaaag augaccucaa ugcccuucug aacgaggugu     1020

ugaagaaugg gacccuaaca uauaaacaga ccaagaaacu gcucggacug ucagacgauu     1080

acgaguuuaa gggugaaaag ggcacuuauu ucaucgaguu uaaaaaguac aaagaguuca     1140

ucaaggcucu uggggagcau aaccugagcc aggacgauuu gaaugaaauu gccaaagaca     1200

uaacacucau caaggaugag auuaaacuga agaaggcauu agccaaauau gaccugaacc     1260

aaaaucagau cgauagucua ucuaagcucg aguuuaaaga ccaccugaac auauccuuca     1320

aggcucuuaa auugguaacu ccccugaugc ucgaaggaaa gaaguacgau gaggcgugua     1380

augaacugaa ccuuaaaguu gcaauuaaug aggacaagaa agauuuucug ccugccuuca     1440

acgagacgua cuauaaggac gaagugacua auccagucgu guugcgggcu aucaaagagu     1500

accgaaaggu gcucaacgcc cuguuaaaga aauauggcaa gguccacaaa aucaauauug     1560

aacuggcacg cgagguuggu aagaaccaua gccagagagc caaaaucgaa aaggagcaga     1620

acgagaauua caaggcuaaa aaggacgccg aacuagagug cgaaaaacuc gggcugaaga     1680

uuaacucgaa aaauauacuu aaguugaggc uguucaaaga gcaaaaggaa uuuugugcau     1740

auucuggaga gaagaucaaa auuuccgauc uccaggacga gaagaugcug gaaaucgauc     1800

acaucuaccc cuauucacgg aguuucgacg auagcuacau gaacaaagug uuaguauuua     1860

ccaagcagaa ucaagagaaa cugaaccaga caccuuucga agcguuuggc aaugacuccg     1920

cuaaguggca gaagauugag gugcuugcca aaaacuugcc aacuaagaaa cagaagcgca     1980

uacucgauaa aaauuacaag gacaaggaac aaaagaacuu caaggauaga aaucugaacg     2040

acaccaggua uaucgcacgu cuaguccuga auuacacaaa agauuaucuc gacuuucugc     2100

cgcuuucuga ugacgagaac accaaguuga augacacgca gaaaggcagc aaggugcaug     2160

uugaggccaa gucagggaug cugaccagug cuuuacggca cacaugggga uucuccgcca     2220

aagaucgaaa caaccaccuc caucacgcaa uugacgcugu gaucauugcc uacgcgaaua     2280

acagcaucgu caaggccuuu ucugauuuca aaaaggaaca ggagucaaau agugcugaac     2340

uguaugccaa aaagauaagc gagcuugacu acaagaacaa aagaaaguuu uucgagcccu     2400

uuuccgguuu caggcagaaa gugcuggaua agaucgacga aauuuucgua ucuaaaccug     2460

agcgcaagaa accaucgggc gcauugcaug aagagaccuu ucggaaggaa gaggaguucu     2520

aucaaagcua cgggggaaag gaaggcguuc ucaaagcucu ggagcuaggu aagaucagaa     2580

aagugaaugg gaagauuguc aaaaacggcg auauguuuag gguggacauc uucaagcaca     2640

agaaaacuaa uaaguuuuac gccgucccca uauauacaau ggauuucgca cugaaagugc     2700

ucccuaacaa ggcgguugcc cgcuccaaaa agggagaaau uaaggacugg auccugaugg     2760

augagaauua cgaauuuugc uucucacuuu auaaagacag uuugauucug auccagacca     2820

aggauaugca ggagccagag uuuguguacu auaacgcuuu cacgucuagc accguauccu     2880

uaauagucuc aaaacaugac aauaaguuug aaacacucag caaaaaccag aagauccugu     2940

ucaagaaugc caacgagaaa gaagugauug caaagucuau cggcauucaa aaucuuaaag     3000

uuuucgagaa guacaucgug uccgcucugg gggaagucac uaaagccgag uuucgacagc     3060

gggaggacuu caagaaaaag cgucccgcag ccaccaagaa agcuggacag gcgaagaaaa     3120

agaaauaagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc acaauuaauu     3180

aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug aucaacaccg     3240

caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca gcuugcuuag     3300

auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa aaaggccaca     3360

aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua aagaaauaaa     3420

ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa uaaaaauuaa     3480

aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaag aauu                                            3564


<210>  3503
<211>  3975
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3503
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaag cgaccugguu cucggacuug auauugguau aggcuccgug gggguaggaa      180

uccugaacaa ggugaccggc gagauuaucc auaaaaauuc uaggauuuuc ccagccgcgc      240

aggcugaaaa caauuugguc cggcgcacaa accgacaagg ucguagauua gccaggcgga      300

agaagcaccg cagagugagg cugaaucggc uauuugagga aucagggcuc aucacugacu      360

ucacgaaaau aaguaucaac cugaauccgu accagcuucg cguuaaggga cugaccgaug      420

aguugagcaa cgaagaacuc uuuauugcac ugaaaaauau ggugaagcau cgaggcaucu      480

ccuaucugga cgaugccucu gacgauggca acucgucagu cggggacuac gcucagauug      540

ugaaagagaa uagcaagcag cucgaaacaa agacucccgg acaaauccag uuagagagau      600

aucagaccua cggucagcug aggggcgauu ucacaguaga aaaagacggg aagaaacacc      660

ggcuuauaaa cguuuuuccu accagugcau aucguuccga ggccuugcgc auucugcaaa      720

cucagcagga guucaaucca cagaucacag augaauuuau uaacagauac cuagagaucc      780

ucaccggaaa gaggaaauau uaccauggcc ccgguaauga aaagucucgg acggacuacg      840

ggcgauaucg cacuagcgga gagacccugg auaacaucuu cggcauucuu auaggcaaau      900

gcacauuuua cccugacgag uucagagcug ccaaggcguc cuauacugca caagaguuua      960

accuguugaa ugaccucaac aaucugaccg ugccaacaga gaccaagaaa uuaucaaagg     1020

aacagaaaaa ccagaucauu aauuacguca agaacgagaa agccaugggg cccgcuaagc     1080

uguucaagua uaucgccaaa cuccuuaguu gugauguggc agacauaaag ggauacagga     1140

uugauaaaag cgguaaggcu gaaauccaca cguuugaggc cuaucggaaa augaagacuc     1200

uggaaacauu ggacaucgag caaauggauc gcgagacccu agacaagcug gcguacgugc     1260

ucacucugaa uacagaaaga gagggcauuc aggaagcccu ugagcacgag uucgcagaug     1320

ggucuuucuc ccagaaacag gucgacgagc ugguucaauu uaggaaggcu aacucaagca     1380

ucuucggaaa aggcuggcau aauuuuagug ugaaguugau gauggagcuc auuccugaac     1440

uguacgagac cucugaagaa cagaugacga uauuaacccg ucuggguaaa cagaagacaa     1500

cuuccagcuc gaacaagacc aaauauaucg augagaagcu acucacagaa gagauuuaca     1560

auccaguagu ggccaaaucu guccggcagg cuaucaagau cgugaacgcc gcaauuaaag     1620

aauaugggga cuucgauaau auaguuaucg agauggcccg agaaacuaac gaggacgacg     1680

agaagaaagc uauucaaaag auccagaagg cgaauaaaga ugaaaaggac gcagccaugc     1740

ugaaagcugc caaccaguac aacggcaagg cagagcuucc gcacuccgug uuucauggac     1800

acaaacaguu ggccaccaag auuagacugu ggcaccaaca gggcgaaagg ugccucuaua     1860

cagggaagac caucucaaua caugaucuga ucaauaacag uaaucaguuc gaggucgacc     1920

acauucuucc ccugagcauc acuuuugaug acuccuuggc uaacaaagug cucguauacg     1980

ccacggcaaa ucaggaaaag ggacaacgca caccuuauca ggcgcuggau ucuauggacg     2040

augcuuggag cuuccgggag uuaaaagccu uuguuagaga gucaaagacc cugaguaaca     2100

aaaagaagga auaccuacuc acugaggaag acauuuccaa auucgaugug aggaagaaau     2160

uuaucgagcg caaucugguc gacacccgau acgcaagccg ggugguccuu aacgccuugc     2220

aggaacauuu ccgugcucac aagauagaua caaaaguguc uguugugaga ggucaauuua     2280

ccucacagcu gaggcgccau uggggcauug agaagacucg ggacacauau caccaccaug     2340

ccguagacgc acucaucauu gcugccagua gccagcugaa uuuauggaag aaacagaaga     2400

acacgcuggu cuccuacucu gaggaucaac uuuuggacau cgaaaccggg gagcucauau     2460

cggaugacga auauaaagag agcguguuca aggcgccaua ccagcacuuc guugauacuc     2520

ugaaauccaa ggaguuugaa gacucaaucc uauucaguua ucagguggau ucuaaauuua     2580

auagaaagau uagcgacgcc acaaucuacg cuaccaggca ggcaaagguc ggaaaagaua     2640

aggccgacga gacauaugug cugggcaaaa uuaaggauau cuacacucaa gacgguuacg     2700

augcuuucau gaaaauauau aagaaggaca aauccaaguu ucucauguac cgacaugacc     2760

cccagaccuu cgaaaaagua auugagccua uccuggaaaa cuauccaaau aagcagauca     2820

acgagaaagg gaaggaggug cccuguaauc cguuucuuaa guacaaagag gaacacggcu     2880

auauucgcaa guacagcaaa aagggaaacg gcccugaaau caaaucauug aaguauuacg     2940

auucuaagcu ggggaaccac auugacauaa cgcccaaaga uuccaauaac aagguugucu     3000

uacagagugu gagcccaugg cgggccgacg uguacuucaa uaaaaccaca ggaaaguaug     3060

agauccucgg ucugaaauac gcagaucuuc aauuugaaaa gggcacuggg accuauaaga     3120

uuucucagga gaaauacaac gacaucaaga aaaaggaggg agucgauucc gacucagagu     3180

ucaaauuuac acuguauaag aaugauuugc uccugguuaa agacacugaa accaaggaac     3240

agcaacuauu cagauuucug aguaggacga ugccuaagca gaaacauuac guggagcuca     3300

agcccuauga uaaacagaag uucgaaggcg gugaggcgcu gauuaaagug cuugggaacg     3360

uagccaauag cggccagugc aagaagggau ugggcaaauc gaacaucucc auauacaagg     3420

uccguacaga cgugcugggg aaucaacaca ucauuaaaaa cgagggagac aagccaaaau     3480

uagauuucaa gcgccccgcu gccaccaaga aagcaggcca ggcuaagaaa aagaaauaag     3540

gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau uaagagugaa     3600

acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc gcaaccuuuc     3660

cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa     3720

auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc     3780

ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa augaaauuca     3840

aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua aaaaugagca     3900

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3960

aaaaaaaaaa gaauu                                                      3975


<210>  3504
<211>  4533
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3504
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaac ccaguucgag ggauuuacaa accuguacca aguuagcaag acucucaggu      180

ucgaacuuau uccacagggu aaaacgcuga agcauauaca ggagcagggc uuuaucgaag      240

aggacaaggc ccggaaugau cacuauaaag aauugaagcc gauuaucgac cgcauuuaca      300

aaaccuaugc ggaucaaugc uuacagcugg ugcagcuaga cugggagaac cucuccgcug      360

ccaucgauuc uuaccgaaag gagaaaacag aagagacucg uaaugcacug auagaggaac      420

aggccaccua uagaaacgcu auccaugacu acuucauugg gaggacagau aaucuuaccg      480

acgcaaucaa caagcggcac gccgagauuu auaagggacu guuuaaagcu gaauuguuca      540

auggcaaggu acucaaacaa cuggguacug ugacaaccac ggagcaugaa aacgcccugc      600

uccgcucauu ugauaaguuc acuaccuacu uuaguggguu cuacgagaau aggaaaaacg      660

ucuuuagcgc ggaagacauc uccacagcaa uaccccacag aauugugcag gauaauuucc      720

cuaaguuuaa agagaacugu cacaucuuca cucgguuaau uaccgccguu ccaucucugc      780

gcgagcauuu cgaaaaugug aagaaggcua ucggaaucuu ugucucgaca ucaauugagg      840

aaguguucag cuuucccuuc uauaaccagc uuuugaccca gacgcaaaua gaccuguaca      900

aucagcuacu cggcggcauc agucgagagg ccgggacuga gaaaauuaag ggacugaacg      960

aaguacuuaa ccuggcaauc cagaaaaaug augagacagc ucacauaauu gccuccuugc     1020

cucauagauu uaucccacuc uucaagcaaa uccugucuga caggaacacc cuuagcuuua     1080

uucuggaaga guucaaaucc gacgaagagg uuauccaguc auuuugcaag uauaagacuc     1140

uccuucggaa ugaaaacgug cuggagacag cggaggccuu guucaaugaa cuaaacagua     1200

uugaucugac ccacauauuu aucagccaca aaaagcucga gacgauuucu uccgcacugu     1260

gugaccauug ggauacccuu cguaaugcuc uguacgaacg cagaaucuca gaguugacag     1320

guaaaaucac uaagagcgcc aaagagaagg uccagaggag ucucaagcac gaagacauua     1380

accugcagga gauaaucucu gcugccggca aagaauuauc cgaggcauuc aagcagaaaa     1440

ccagcgaaau ucugucgcau gcccacgcug cgcuagauca accccucccu acaacucuga     1500

agaaacagga ggagaaggaa auccuuaagu cucaguugga cucccugcuc gggcuguauc     1560

aucuucugga uugguuugca guggacgagu caaaugaagu ggauccagag uucagugccc     1620

gguugaccgg aauuaaacuc gaaauggagc cgagccuguc cuucuacaac aaggcucgaa     1680

auuaugccac uaaaaagccc uacucugucg agaaauuuaa guuaaacuuc caaaugccua     1740

cccuggcaag cggcugggac guuaauaaag aaaagaacaa uggugccauc cuauuuguga     1800

agaacgggcu cuacuaucug ggaauaaugc caaaacagaa gggccgcuac aaagcucuuu     1860

cauucgagcc cacugaaaag acgagugagg gcuuugauaa aauguauuac gacuauuucc     1920

cugacgccgc aaagaugauc ccaaagugcu ccacacaguu gaaagcggua accgcucacu     1980

uucagacuca caccacaccc auucugcuca gcaacaauuu caucgaaccg cuggagauua     2040

ccaaggagau cuacgauuua aacaauccug aaaaagagcc caagaaauuu caaacugccu     2100

augcaaagaa gacaggggac cagaaaggau acagagaagc ccuguguaag uggauagauu     2160

ucacgaggga cuuucuuucu aaauacacca agacuacauc aauugauuug aguagccucc     2220

ggccauccuc ucaguauaaa gaccugggug aguacuaugc ugagcuaaac ccucugcucu     2280

accauaucuc guuccagcgc auugccgaaa aggagaucau ggaugcagug gaaaccggca     2340

agcuguaucu uuuucaaaua uacaauaaag acuucgcuaa ggggcaccau ggaaaaccca     2400

acuugcacac acuguauugg acuggcuuau ucagcccaga gaaucucgcc aagaccucca     2460

ucaaacugaa cggucaggcg gaauuauuuu acagacccaa gucaaggaug aaacguaugg     2520

cccaccggcu gggggagaag auguugaaua agaaacucaa ggaucagaaa acgccuauuc     2580

cagacacccu guaccaagag cuauaugauu acgucaacca ucgacugagu cacgaccucu     2640

cugaugaagc uagagcacug cuuccgaaug ugaucacaaa ggagguuagc caugaaauua     2700

ucaaagacag gcgcuucacu uccgacaagu uuuucuuuca cgugcccaua accuugaacu     2760

aucaggccgc uaauucaccu agcaaguuca accagcgggu caaugccuac cugaaagagc     2820

auccagaaac acccauuauc ggcaucgaua gaggagagag gaacuuaauu uauaucacug     2880

ugauugacuc uaccggcaag auacucgagc agcgcucccu gaacacgauc caacaguuug     2940

auuaccagaa aaagcuggac aaucgagaaa aagagcgggu agcagcgcgu caggccugga     3000

guguuguggg gacaauuaag gaucuuaagc aaggauaucu aagccagguc auccacgaaa     3060

uuguggaccu caugauccac uaccaggcug ucgugguucu ggagaacuug aauuucgguu     3120

uuaaaucuaa gagaaccggc auagccgaaa aagcagugua ccagcaauuc gagaagaugc     3180

ugaucgauaa auuaaacugc cucguacuga aggacuaucc ugcugagaag gucgggggag     3240

ugcuuaaucc auaccagcug acugaucagu uuacauccuu cgccaaaaug ggcacccaau     3300

cagguuucuu guuuuauguu cccgcaccgu acacaaguaa gauugacccu cucaccgggu     3360

ucguggaucc auuugucugg aaaacaauca agaaccauga aagcaggaaa cacuuccugg     3420

agggcuuuga cuuccuacau uaugauguga agacgggaga cuuuauucug cacuucaaga     3480

ugaaucgcaa ccuuucguuu cagcggggcc uccccggguu caugccugcc ugggacaucg     3540

uauuugagaa aaaugaaacc caguucgaug cuaagggaac ucccuuuaua gcggguaaaa     3600

gaauugugcc aguuaucgag aaccauaggu ucacaggccg auaccgcgac cuguauccug     3660

ccaaugaauu gaucgcacug uuagaggaaa aggggauugu cuuccgggau ggauccaaca     3720

uacuccccaa acugcuugag aaugacgauu cucacgcuau cgacaccaug guggcccuga     3780

uuagaagcgu guugcagaug aggaacucaa augccgcuac aggcgaagau uacaucaaca     3840

guccaguccg ugaccucaac gguguuuguu uugauucccg cuuccaaaau ccggaguggc     3900

cuauggacgc agaugccaac ggggcguacc acauugcucu gaagggccag cuacugcuua     3960

aucaucucaa agagucuaag gaccugaagu ugcagaacgg aaucagcaau caggacuggu     4020

uagccuauau acaagaacug cggaacaaaa gacccgcagc cacuaagaaa gcuggccagg     4080

caaagaaaaa gaaguaagga cuagugaagg gaacacccaa auuuaauuca gccuuaagca     4140

caauuaauua agagugaaac guaauuguac aagcaguugg ucacccacca uagggcauga     4200

ucaacaccgc aaccuuuccu uuuuccccca gugauucuga aaaaccccuc uucccuucag     4260

cuugcuuaga uguuccaaau uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa     4320

aaggccacaa aaguucccuc ucacuuucag uaaauaaaau aaaagcagca acagaaauaa     4380

agaaauaaau gaaauucaaa augaaauaaa uauuguguug ugcagcauua aaaaaucaau     4440

aaaaauuaaa aaugagcaag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  4533


<210>  3505
<211>  4512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3505
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaag cauuuaccag gaguucguua acaaguauuc ccugucuaaa acccucaggu      180

uugaacuuau accacaagga aagacacugg agaauaucaa ggcccggggu uugauuuuag      240

acgaugaaaa acgcgcgaag gacuacaaaa aggcuaaaca gaucauugau aaguaucauc      300

aguucuuuau cgaggaaaua cugucaagug ugugcaucag cgaggaccua cuccagaacu      360

acuccgaugu auauuucaag cugaaaaagu cugacgauga caaucuucaa aaagauuuua      420

agucggccaa agacacuauu aagaaacaga ucucagagua cauuaaggau agcgaaaagu      480

ucaaaaaccu guuuaaucag aacuugaucg acgcaaagaa aggccaggag agugaucuca      540

uacuguggcu gaagcaaucc aaagacaaug ggauugaacu cuucaaggcc aacucugaca      600

ucacggauau ugacgaggcu uuagagauca ucaagagcuu uaaaggaugg accacauauu      660

ucaagggcuu ucacgaaaau cguaaaaacg uguacuccuc aaaugauauu ccgacaagua      720

uaaucuaccg aauugucgac gauaaccugc ccaaguuccu ugagaauaaa gcaaaguaug      780

agagcuugaa ggacaaagcc ccugaggcua ucaacuacga acagauaaag aaagaucugg      840

ccgaggagcu aaccuuugac auugauuaua agacaucuga agugaaucag agaguuuucu      900

cccucgacga gguguucgaa aucgcgaacu uuaacaauua ccugaaccag ucagguauca      960

ccaaauucaa uacuauuauc gggggaaagu uugucaacgg cgagaauaca aagaggaaag     1020

gcauuaacga auauauaaau cuuuacagcc aacagaucaa cgauaagacc cugaaaaagu     1080

auaaaaugag uguguuguuc aagcagauuc ucucugacac ggaguccaag agcuuuguaa     1140

ucgacaaacu ggaggaugac ucggauguug ugacuaccau gcaaucuuuc uacgaacaga     1200

ucgcagccuu uaagacaguc gaggaaaaau ccauuaagga gacuuuauca cuccuguucg     1260

acgaucuuaa agcucagaag cuggacuuga guaaaauaua cuuuaagaau gauaagagcc     1320

uaaccgaccu gucccagcaa guguucgaug acuauucugu gaucgggaca gccguccucg     1380

aauacauuac ccagcagauc gcacccaaaa accuggauaa uccaagcaag aaagagcagg     1440

agcuuauugc uaagaaaacg gaaaaggcca aguaucuguc auuggagacu aucaaacucg     1500

cgcuggaaga guuuaacaag caucgggaca uagauaaaca augucgcuuc gaggaaaucu     1560

uagccaauuu ugcagcuauu ccuaugaucu ucgacgagau ugcccagaac aaggacaauc     1620

uggcucagau caguauaaaa uaccagaacc aaggaaagaa ggaucuacuc caggccuccg     1680

cagaagacga uguuaaagcc auuaaggacc ugcuugauca gacaaacaau uugcugcaca     1740

aacucaagau cuuccauauu agccagucug aggacaaagc uaacauccug gauaaggacg     1800

aacacuuuua ucuuguguuc gaggagugcu acuuugaacu ggcgaauaua guaccauugu     1860

auaacaaaau cagaaauuac auuacccaaa agcccuacuc agaugagaag uucaaacuca     1920

acuuugaaaa uaguacucug gcaaacgguu gggacaagaa uaaagagccu gauaacacag     1980

ccaucuuauu cauuaaggac gauaaauauu accugggcgu gaugaauaag aagaacaaua     2040

aaaucuuuga cgacaaggcu auaaaagaaa acaaggggga gggauauaaa aagauugucu     2100

acaagcuacu cccaggcgcc aauaaaaugc ugccgaaggu guucuuuagc gcaaaaucca     2160

ucaaguucua uaaccccucu gaggauaucc uuaggauucg gaaccacucg acccauacga     2220

aaaaugguag cccucagaag ggguacgaaa aguuugaguu caacaucgaa gacugucgca     2280

aauuuauuga uuucuauaag caguccauau caaaacaccc agaguggaag gacuucggau     2340

uucgauucag ugauacccaa agauacaauu cuaucgacga auuuuacagg gagguugaga     2400

accagggcua uaaauugaca uucgaaaaua uuagcgaguc cuacaucgau ucagugguca     2460

accagggcaa gcuguaucuc uuucagauuu acaauaagga cuucagcgcc uauucuaaag     2520

ggcggcccaa ccugcauacu uuauacugga aggcucuguu ugaugaacgu aaucuucaag     2580

acgugguaua uaaauugaac ggagaggccg aacucuucua ccgcaagcag uccaucccua     2640

aaaagauaac ccacccagca aaagaagcga ucgcuaauaa gaacaaggau aaucccaaaa     2700

aggagagugu uuuugaauac gaccugauua aagacaagag auucacagag gauaaauuuu     2760

ucuuccacug cccgaucacu auuaacuuua agagcucugg ugccaauaag uucaacgacg     2820

aaaucaaccu acugcucaaa gagaaggcaa augaugugca uauacugucc auugacaggg     2880

gcgagcggca ccuugccuau uacaccuugg ucgaugggaa aggaaacauc aucaagcagg     2940

acacauuuaa uauuauaggc aacgaucgaa ugaaaaccaa uuaucaugac aagcuggcug     3000

ccaucgaaaa ggaucgcgac ucagcaagaa aagauuggaa gaaaauuaac aauaucaagg     3060

agaugaaaga agguuacuua agucaggugg uccacgagau ugcuaagcuc gugaucgaau     3120

auaacgccau aguuguguuc gaggaccuga auuuuggguu caagaggggc cgguuuaaag     3180

uagagaagca agucuaccag aaacuugaaa agaugcugau cgagaaauug aacuaucucg     3240

uguucaagga uaaugaauuu gacaaaacug gaggcguucu gcgcgcguac cagcuaacgg     3300

ccccuuucga gacauuuaag aagaugggga aacagaccgg aauuaucuac uaugugcccg     3360

cugguuucac uagcaagauu uguccaguca ccggcuuugu gaaccaacug uacccuaaau     3420

augaaucggu auccaagucu caggaguucu ucagcaaauu ugacaagauc ugcuacaauc     3480

ucgauaaggg guauuucgag uuuucauucg acuacaaaaa cuuuggagau aaggcagcca     3540

aaggcaagug gacaauagcu aguuucgguu ccagacugau uaauuuuagg aacucugaca     3600

aaaaucauaa cugggauacc cgugaagugu aucccacuaa ggagcuugaa aaguugcuga     3660

aagacuacag caucgaguac gggcacggcg aguguaucaa ggccgcaauu ugcggagaau     3720

ccgauaaaaa guucuuugcg aaauuaacau caguucucaa cacgauccug cagaugcgga     3780

auagcaagac cggcacugag cuugacuauc ugauaagucc agucgccgau gugaacggga     3840

auuucuuuga cucucgacaa gcucccaaga acaugccuca ggaugccgac gcaaauggag     3900

cuuaccacau uggucuaaaa ggccucaugc uguuggggag aaucaagaac aaucaggaag     3960

gcaaaaagcu gaacuuagug auuaaaaaug aggaauauuu cgaguuuguc cagaacagga     4020

auaacaagcg cccagccgca acuaaaaagg ccggacaagc uaagaaaaag aaauaaggac     4080

uagugaaggg aacacccaaa uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg     4140

uaauuguaca agcaguuggu cacccaccau agggcaugau caacaccgca accuuuccuu     4200

uuucccccag ugauucugaa aaaccccucu ucccuucagc uugcuuagau guuccaaauu     4260

uaguaagcuu aaggcggccu acagaagaaa aagaaaaaaa aggccacaaa aguucccucu     4320

cacuuucagu aaauaaaaua aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa     4380

ugaaauaaau auuguguugu gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga     4440

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaagaa uu                                                         4512


<210>  3506
<211>  4813
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3506
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagacaa gaaauacagc      240

auuggacugg auauagguac aaacuccguu ggcugggccg ugaucacaga cgaguauaag      300

guaccaucua agaaauucaa ggugcucggg aauacugaua ggcauucaau uaaaaagaac      360

cuuaucggag cgcuguuguu ugacaguggc gaaacggcug aggccacccg guuaaaacgc      420

acagcacgac guagauacac uaggcggaag aaucgcauuu gcuaucugca ggaaaucuuc      480

agcaacgaga uggccaaggu cgaugacucc uuuuuccaca gacuagaaga gucuuuucuc      540

guggaggaag auaaaaagca ugagaggcac ccgauauucg guaauaucgu ugacgaagug      600

gcuuaccaug agaaauaucc caccauuuac caucugcgga agaaacuugu cgauucgaca      660

gacaaggcag aucugcgcuu gaucuaucuc gcccuggcuc acaugauuaa auuucgaggg      720

cacuuccuga ucgagggaga ccucaacccu gauaauucag acguggacaa guuauuuaua      780

caacugguac agaccuacaa ccagcuuuuc gaagagaauc caauuaacgc cagcggcguu      840

gaugcgaagg caaucuugag ugccagacug uccaaaucua ggcggcuaga aaaucucauu      900

gcucagcugc ccggcgagaa gaaaaacggg cuuuuuggaa aucugaucgc cuugagccuc      960

ggucugacuc cuaacuucaa guccaauuuu gacuuagcag aagaugcuaa acugcaacuc     1020

ucaaaggaca cauacgauga cgaucuugac aaccuguugg cccagaucgg cgaucaguau     1080

gcggaccuau uccuggccgc aaagaaccuc agugaugcua uucugcuuag cgacauacug     1140

cgugugaaua ccgagaucac gaaagcccca uugucugcuu ccaugauuaa gcgcuacgac     1200

gagcaccauc aggaucucac ucuguuaaaa gcccugguca gacaacagcu acccgaaaag     1260

uauaaagaga ucuucuuuga ccagucaaag aacggguacg caggauauau agauggcggu     1320

gccagccagg aagaguucua caaguuuauu aaaccuaucc ucgaaaagau ggacgggacc     1380

gaggagcugc uugugaaauu gaauagggaa gaucugcucc ggaagcaacg aacauucgac     1440

aacggaagua ucccacacca gauucaccug ggcgagcuuc augcuauccu gcgcagacag     1500

gaagauuuuu auccguucuu gaaagacaau agggagaaga uugaaaagau acucacuuuu     1560

cggauccccu acuacguggg cccucuggcg cgcgggaacu cuagauucgc auggaugacc     1620

aggaaauccg aggagacaau uaccccaugg aauuuugaag aggucguuga uaagggagcc     1680

agcgcucaau cguucaucga acguaugacg aacuuugaca aaaauuuacc caacgagaag     1740

gugcugccua aacacucucu acucuaugag uacuucacug uauauaauga acugacaaag     1800

gugaaauacg ucaccgaggg uaugcggaag ccagccuuuc uuuccggcga acagaagaaa     1860

gcaaucgugg auuugcuguu caagacuaac cgaaaaguua cagugaagca gcucaaagag     1920

gacuauuuca agaagauuga auguuuugau ucagucgaga uaaguggggu ggaggacaga     1980

uucaaugcca gccugggaac cuaccaugac uuacugaaaa ucauuaagga uaaagacuuu     2040

cuugauaacg aagagaauga agacaucuug gaggauauug uacucacgcu gacccuauuc     2100

gaagacaggg agaugaucga ggaacgccug aagacauaug cucaccucuu ugaugacaaa     2160

guuaugaagc agcugaagcg gagaagguac acuggcuggg gucgccuuuc ccgaaaauug     2220

auaaacggga uccgggauaa gcaaucuggc aaaaccauuc uggacuucuu aaagagcgau     2280

ggauuugcca accguaauuu caugcagcuc auccaugacg auucacugac auuuaaagag     2340

gacauucaga aggcacaggu gaguggccaa ggggacuccc uucacgaaca caucgcgaac     2400

cuggcuggaa gccccgccau aaagaaaggu auuuugcaga cugucaaggu ggucgaugag     2460

cucgugaaag uuaugggcag acauaagccg gaaaauaucg ugauugagau ggcaagggag     2520

aaccagacca cacagaaagg gcaaaagaac ucucgcgaac ggaugaaaag aaucgaggaa     2580

ggaauaaagg agcugggcuc acagauccua aaggagcacc cuguagaaaa uacccagcug     2640

cagaaugaga aacucuaccu guauuaccuu caaaacggua gggacaugua ugucgaucag     2700

gaauuggaca uuaaucgacu gagugauuac gacguggauc auaucguucc ccagagcuuc     2760

uuaaaggacg auuccauuga caacaaagug cucacucgcu cugauaagaa ucgggggaaa     2820

ucggacaacg ucccaagcga ggaaguggua aagaagauga aaaauuauug gagacaacug     2880

cuuaacgcca agcugaucac gcagaggaaa uuugacaauc uaacaaaggc ugagcguggc     2940

ggacucuccg agcuggauaa agccggcuuc auaaagcgcc aguuggugga aacccggcag     3000

auuacuaagc acguugcaca aauccuggac ucaagaauga acaccaaaua cgaugagaac     3060

gacaaguuaa ucagggaagu caaagugauu acacucaaga guaaacuggu gucugauuuu     3120

cgaaaggacu uccaguucua uaagguccgg gagaucaaua acuaccacca ugcucacgau     3180

gccuaccuua augcgguugu ggggaccgcc cugauuaaaa aguauccuaa auuggaaagc     3240

gaguuugugu acggagacua uaagguauac gauguccgca aaaugauagc uaaguccgag     3300

caggaaaucg guaaggcaac ugccaaauau uucuuuuacu caaacauuau gaauuucuuu     3360

aagacagaga ucacgcucgc uaacggcgaa auuagaaaaa ggccccugau cgagaccaau     3420

ggggaaacug gagagauagu gugggacaag ggccgggauu ucgccacagu ucgaaaagug     3480

cuaagcaugc cacaggucaa caucgugaag aaaaccgagg uucaaacagg uggguuuucu     3540

aaggaaucca uucugcccaa gagaaauagu gacaaacuua ucgcacgcaa gaaagauugg     3600

gacccuaaga aauauggcgg auucgacagc ccaacugugg cguacucugu acucgucgug     3660

gccaaggugg agaagggcaa auccaagaaa cugaagucag ucaaagaauu gcuggggauu     3720

accaucaugg agaggaguag cuuugagaag aacccgauag auuucuuaga agcuaaggga     3780

uacaaagagg uuaagaaaga ccucauuauc aagcugccca aauauucgcu uuuugaacug     3840

gagaauggcc guaagcggau guuggccucc gcuggugaac uccagaaggg gaacgagcug     3900

gcacuaccuu cuaaauacgu gaauuuccug uaucuugcca gccauuacga gaagcucaaa     3960

ggaucaccag aagauaacga gcagaagcag cuguucguag aacaacacaa acauuauuug     4020

gacgagauca uugaacagau aagugaguuu uccaagagag ugaucuuagc agaugccaau     4080

cuggacaaag uccucucugc uuacaacaag cacagggaua agcccauucg cgagcaggcg     4140

gaaaacauca uucaccuguu cacgcuuacc aaucugggcg ccccugcagc uuuuaaauau     4200

uucgacacaa cuaucgaucg aaagagauac accagcacaa aagagguguu ggacgccacu     4260

cuaauacauc aauccaucac cggucuguac gaaacgcgga uugaucucuc acagcugggc     4320

ggggacaaga ggccagccgc uaccaaaaag gcaggacagg ccaagaaaaa gaaauaagga     4380

cuagugaagg gaacacccaa auuuaauuca gccuuaagca caauuaauua agagugaaac     4440

guaauuguac aagcaguugg ucacccacca uagggcauga ucaacaccgc aaccuuuccu     4500

uuuuccccca gugauucuga aaaaccccuc uucccuucag cuugcuuaga uguuccaaau     4560

uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa aaggccacaa aaguucccuc     4620

ucacuuucag uaaauaaaau aaaagcagca acagaaauaa agaaauaaau gaaauucaaa     4680

augaaauaaa uauuguguug ugcagcauua aaaaaucaau aaaaauuaaa aaugagcaag     4740

aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4800

aaaaaaaaga auu                                                        4813


<210>  3507
<211>  5596
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3507
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaacuu caagauucug      240

ccaauagcca ucgaccucgg aguuaaaaau accggugugu uuagcgcguu cuaccagaag      300

ggcacauccc uugagaggcu ggauaacaag aaugggaaag uauaugaauu gucuaaggac      360

ucauacacuu uacugaugaa caaucggacg gcucgccgac aucaacguag aggaauugau      420

aggaaacagc uagugaagcg gcucuuuaaa cugaucugga ccgagcagcu uaaccuggaa      480

ugggacaagg auacacagca agccauuagu uucuuguuua aucgcagagg cuucagcuuu      540

aucacugacg guuauucccc ggaguaccuc aacauagucc ccgaacaggu gaaggcaauc      600

cugauggaua uuuucgacga uuauaauggg gaggacgauc uggacucuua ccucaaauua      660

gccaccgagc aggaaucgaa gaucucagag auuuauaaca aacugaugca gaagauccuu      720

gaauuuaaau ugaugaagcu gugcacagau auaaaagacg acaagguuag caccaagacu      780

cuaaaagaga uuacaaguua cgaguucgaa cuccuggcug auuaccuugc aaauuauucc      840

gagucucuga agacccaaaa auuuagcuac acggacaagc agggaaacuu gaaagaacuc      900

uccuauuacc accaugauaa guauaauauc caggaguucc ugaagaggca cgccacuauu      960

aacgaccgga ucuuagauac ccugcucaca gacgaucuug acaucuggaa uuuuaacuuc     1020

gaaaaauucg auuuugacaa gaacgaggag aaacugcaga aucaagaaga uaaggaccau     1080

auucaggcuc acuugcacca uuucguguuu gccgucaaca aaauaaaguc agagauggcg     1140

aguggcggcc gccaccgaag ccaguacuuc caagaaauca cuaaugugcu agacgagaac     1200

aaucaucagg aaggguaucu gaagaacuuu ugugagaauc uccacaacaa aaaguacucu     1260

aaucuguccg uuaaaaaccu uguaaaucug auuggaaacu ugucaaaucu cgagcugaag     1320

ccuuuaagaa aauacuucaa cgauaagauc cacgcaaagg ccgaccauug ggaugaacag     1380

aaauuuaccg agacauauug ccacuggaua cugggugaau ggaggguggg cgucaaggac     1440

caggauaaaa aggacggggc uaaauacagc uauaaggauc uauguaauga gcucaaacaa     1500

aaggugacca aggccggacu gguggacuuc cuuuuggaac uggauccaug ccggacgauu     1560

cccccuuacc ucgacaacaa caaucguaaa ccacccaagu gucagagucu gauccuuaac     1620

ccuaaauuuc uggauaauca guauccaaac uggcagcaau acuugcagga gcucaagaaa     1680

cugcagucua uccagaauua uuuagacucc uucgagacug aucugaaggu ccuaaagagc     1740

ucgaaagacc aaccguacuu uguugaauac aagucuucca accagcagau ugcaucaggc     1800

cagcgcgacu auaaagaucu cgacgcuaga auccugcaau ucauuuuuga uagggugaag     1860

gccagugacg agcuuuugcu gaaugaaaua uacuuccagg cgaaaaagcu caagcagaaa     1920

gccagcuccg agcuggagaa guuagaaucu agcaaaaagc uggaugaggu aaucgcaaac     1980

ucacaacuua gucagauuuu gaaaucccag cauacaaaug guaucuucga acaggggacc     2040

uuucuccacc uggugugcaa guauuacaag caacggcagc gagcucgcga cagcagacua     2100

uauaucaugc ccgaguacag guaugauaaa aagcugcaua aauacaacaa uacuggacgg     2160

uucgacgaug acaaccagcu ccugacauac uguaaucaca agccucgcca gaaaagauau     2220

caacuuuuga acgaucuggc cggcgucuua caggugucuc caaauuuucu caaggacaaa     2280

auuggcucag augacgaccu guucauaagu aaguggcuug uugaacacau caggggguuu     2340

aagaaagcau gcgaggauag ccugaagauu cagaaagaca accguggauu gcucaaccau     2400

aagaucaaua uugcccggaa caccaaaggu aagugugaga aggaaaucuu caaucugaua     2460

ugcaaaaucg agggcuccga agauaagaaa gggaacuaca agcacggacu agcauaugag     2520

cugggcgugc uccuguuugg ugaacccaau gaggccucua aaccugaguu cgaccgaaag     2580

auuaagaaau uuaacucgau cuacagcuuc gcucagauuc aacagaucgc guuugcagaa     2640

agaaagggga augccaacac gugugcuguc ugcuccgccg auaaugcaca uaggaugcag     2700

caaauaaaaa uuaccgagcc aguggaagac aacaaggaua aaaucauucu uucagccaag     2760

gcucagcgcu ugcccgccau cccgacacgg auaguagacg gcgcaguuaa gaaaauggcg     2820

acuauccugg cuaagaauau uguggaugac aacuggcaga auaucaaaca ggucuuaagu     2880

gccaagcacc aacuccacau uccuaucaua accgagucua acgcauucga auuugagccc     2940

gcccuggcug augugaaagg aaagagccuu aaggacagaa ggaaaaaggc ccuggagcgc     3000

auuuccccag aaaauaucuu caaagacaag aacaaccgaa ucaaagaguu cgcaaagggc     3060

auuucagcuu auagcggggc caaucuaaca gauggagacu uugauggugc gaaagaagaa     3120

cucgaccaua ucauuccucg gucucacaag aaguacggca cucugaacga ugaggccaau     3180

uugauaugcg ucacccgugg ggacaacaaa aauaagggaa acagaaucuu cugucugagg     3240

gauuuagcug acaauuauaa acucaagcag uuugaaacaa ccgaugaccu ggagauugag     3300

aaaaagaucg cagauacuau uugggacgcc aacaagaaag auuucaaguu uggcaauuac     3360

cgcuccuuca ucaaccuuac gccccaggag cagaaggcuu uucggcaugc ccuguucuug     3420

gcagacgaaa auccaauaaa acaagcggug aucagagcca uuaacaauag gaaccgaaca     3480

uuuguuaaug guacccagcg cuacuucgcu gaggugcucg ccaacaauau cuaucugcgg     3540

gcuaaaaagg agaaccuaaa cacugacaag auuaguuuug auuacuucgg gauccccacc     3600

auaggcaaug gaagaggcau ugcugaaauc aggcagcugu augagaaagu agacagcgau     3660

auccaggccu acgcaaaggg ggacaaaccu caagccucuu auucccaccu uauugaugcu     3720

augcucgcgu ucugcauagc cgcagacgaa caucguaacg auggaucaau cggucuggag     3780

auugacaaga auuacaguuu guauccacug gauaaaaaca caggcgaagu cuuuaccaag     3840

gacaucuuca gccagauuaa gaucacugau aaugaguuuu cggacaaaaa guuagugcgc     3900

aaaaaggcua uagagggguu caacacacac cggcagauga cgagagaugg aaucuacgcc     3960

gaaaauuacc ucccgauucu gauccacaaa gagcuuaacg aaguuaggaa gggcuauacc     4020

uggaaaaauu ccgaggaaau uaagaucuuu aaggguaaaa aguacgacau acagcaacug     4080

aacaauuugg uguauugucu caaauucguc gacaagccca uuucuaucga uauccagauu     4140

agcacucugg aggagcuacg aaacauccug acaaccaaua acauagccgc uacagcagaa     4200

uacuauuaca uuaaucuuaa aacucagaag cuccaugagu auuacaucga aaacuacaac     4260

accgcccugg gguauaagaa auacucaaag gagauggagu uuuugcggag uuuagcguau     4320

cgcuccgaaa gagugaaaau uaagucuauc gacgauguaa aacaagugcu ggacaaggau     4380

agcaauuuca uaauuggcaa gaucacgcuc ccuuuuaaaa aggaguggca gaggcuguac     4440

cgggaauggc agaacaccac aaucaaagac gauuaugagu uccuuaaguc cuuuuucaau     4500

guuaaaucaa uuacuaagcu gcacaagaaa guccgaaagg acuuuagcuu gccaaucagu     4560

accaacgaag gaaaauuccu agugaagaga aaaacauggg auaauaacuu cauuuaccag     4620

auacugaaug acucugauuc ccgcgcugac ggcacuaagc ccuuuauccc ugccuucgac     4680

auuagcaaaa acgagaucgu ggaggcaauc auugauucgu uuaccucaaa gaauauauuc     4740

uggcucccaa agaacaucga acugcaaaaa gucgacaaua agaacauuuu ugccaucgau     4800

acgucuaaau gguucgaggu ugaaacaccc agugaccuua gggauauugg gaucgcuacc     4860

auacaguaua agaucgacaa uaacucccgu ccgaaagugc gggugaaguu agauuacgua     4920

auugacgaug acagcaagau caauuacuuu augaaccauu cucugcucaa aagcagauau     4980

ccugauaagg ucuuggagau ucugaaacag uccacuauca uagaguucga aucaagugga     5040

uuuaacaaga caauuaaaga gaugcucggc augaagcugg cagguaucua caaugaaacc     5100

agcaacaaua agaggccagc cgcgacuaaa aaggcugggc aggccaaaaa gaaaaaguaa     5160

ggacuaguga agggaacacc caaauuuaau ucagccuuaa gcacaauuaa uuaagaguga     5220

aacguaauug uacaagcagu uggucaccca ccauagggca ugaucaacac cgcaaccuuu     5280

ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu cagcuugcuu agauguucca     5340

aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa aaaaaggcca caaaaguucc     5400

cucucacuuu caguaaauaa aauaaaagca gcaacagaaa uaaagaaaua aaugaaauuc     5460

aaaaugaaau aaauauugug uugugcagca uuaaaaaauc aauaaaaauu aaaaaugagc     5520

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5580

aaaaaaaaaa agaauu                                                     5596


<210>  3508
<211>  3868
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3508
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagag gaacuacauu      240

cugggacucg acauagguau cacaucaguu ggcuauggga uuaucgauua cgagacacgg      300

gacgugauug augccggagu acgccuuuuc aaagaagcga auguggagaa caaugaaggc      360

cgacguucca agagaggugc uaggcggcug aagcgcagaa ggcggcaucg cauccagcga      420

gucaaaaagu uguuauuuga cuauaaccug cuaacugauc acucugagcu cucagggaua      480

aauccauacg aagccagagu gaaaggacug agucaaaaac uuagcgagga ggaguucucc      540

gcagcccugu ugcaucucgc uaaaaggcgg ggcguucaca acgugaauga agucgaagag      600

gacacgggca acgagcuguc uaccaaggaa cagaucucgc guaauucaaa ggcacuggag      660

gaaaaauaug uggccgagcu ccaguuagaa cgccugaaga aagaugggga gguaagagga      720

agcauuaaca gguuuaagac aagugacuac guuaaagagg cuaagcagcu uuugaaagug      780

caaaaggccu aucaucagcu ggaucagucc uucaucgaca cuuacauuga ucuacucgaa      840

acccggcgaa cauacuauga ggguccgggc gaagggucuc ccuuuggaug gaaggacauc      900

aaagaguggu acgaaaugcu gaugggccac ugcaccuauu ucccugagga gcuucgcagc      960

gucaaguacg cguauaaugc agaucuguac aacgccuuga augaccucaa caaucuggug     1020

auaacuagag acgaaaacga gaaauuagaa uauuacgaga aguuucagau uaucgaaaac     1080

guguucaaac aaaagaagaa accaacacug aagcagauug cuaaagagau ccucgucaau     1140

gaggaagaua ucaaggguua caggguuacc uccacgggga aacccgaguu uacuaaccuu     1200

aagguguauc acgacauuaa ggauauaacc gcccggaaag aaaucauuga gaaugcagag     1260

cuguuggacc agaucgcuaa gauacuaaca auuuaccagu caagugaaga uauccaagag     1320

gaacugacua accucaauag cgagcugacc caggaagaga ucgagcagau uucuaaccuu     1380

aaaggauaua caggcaccca uaaucugucc uugaaggcca ucaaccucau ucuggacgaa     1440

uuauggcaca cgaaugauaa ccaaauagcg aucuucaauc gccugaaacu uguaccuaag     1500

aagguggacc ucucacagca gaaagagauu ccaacuacac uggucgauga cuuuauccuu     1560

agccccgugg uuaagagaag uuucauccag ucuauuaaag ugauaaacgc caucauuaag     1620

aaauacggcu ugccuaauga uaucauuauc gaacuggcaa gggagaagaa cuccaaagac     1680

gcucaaaaga ugauaaauga aaugcagaag cguaaccggc agaccaacga gcgaaucgag     1740

gaaauuauca gaacuacagg gaaagagaau gccaaguauc ucauugaaaa gaucaagcug     1800

caugacaugc aggagggaaa augucuuuac agccuggaag cuauaccauu ggaggaucuc     1860

cugaacaauc cguuuaacua ugaggucgac cacauuaucc ccaggucggu gucuuucgau     1920

aauuccuuca acaauaaggu auuaguuaag caagaagaga acucaaagaa ggguaaucgc     1980

accccuuuuc aguaccugag uagcuccgac ucuaaaauua gcuacgaaac guucaagaaa     2040

cacauccuaa accucgccaa gggcaagggg cggauaucaa aaaccaagaa agaguaucug     2100

cuugaggaaa gagauaucaa uagguuuagu gugcagaagg acuucauuaa ccgcaauuug     2160

gucgauacac gauacgcaac ucggggacug augaaccucc ugcguuccua uuuuagagug     2220

aauaacuuag acgucaaagu gaaaagcauc aacggcgguu ucaccucuuu ucugaggcgc     2280

aaguggaaau ucaagaaaga gcggaauaag ggguacaaac aucacgccga agaugcucuu     2340

auuaucgcga acgcagacuu uauauucaag gaguggaaaa aguuggauaa ggccaaaaag     2400

guuauggaaa aucagauguu ugaggagaaa caagcugaau caaugccaga gauugaaaca     2460

gagcaggaau auaaggagau cuucaucacu ccccaucaga uuaaacacau caaggacuuu     2520

aaggauuaca aauauaguca uagaguggac aagaaaccua acagggagcu cauuaaugac     2580

acccuguaca gcacacgaaa ggaugacaaa ggcaacaccc uaauaguaaa uaaccugaau     2640

ggacucuacg auaaggacaa cgauaagcug aaaaagcuaa ucaauaaauc cccagaaaag     2700

uugcugaugu aucaccacga cccccagacu uaccaaaaau uaaagcucau uauggagcag     2760

uauggcgaug aaaagaaccc gcuguacaaa uauuacgagg aaacggggaa uuaucuuaca     2820

aaguacucua aaaaggacaa cggaccuguc aucaaaaaga uuaaguacua ugguaauaaa     2880

cugaacgccc auuuggauau caccgacgau uaccccaacu cgcgcaauaa ggugguuaaa     2940

cucagccuga agccauaucg guucgacgug uaccuagaua acggcgucua uaaauucgug     3000

acuguaaaga aucuggacgu gaucaaaaag gagaacuacu augagguuaa uuccaagugc     3060

uacgaagagg ccaaaaagcu caaaaagaua ucaaaccagg cagaauuuau ugcuaguuuc     3120

uacaauaacg accugaucaa aauuaauggg gagcuuuaua gagucaucgg agugaacaau     3180

gauuugcuga acaggauaga ggugaauaug auugacauca ccuaccguga auauuuagag     3240

aacaugaaug auaagcgccc uccccggauc auuaagacaa uagccucuaa aacccaaagc     3300

aucaagaaau acuccacuga cauucucggc aaccuguaug aagucaagag caaaaagcac     3360

ccacagauca uuaagaaagg uaagagaccc gcagcgacca aaaaggcugg gcaggccaaa     3420

aagaagaaau aaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu     3480

aauuaagagu gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac     3540

accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc     3600

uuagauguuc caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc     3660

cacaaaaguu cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa     3720

uaaaugaaau ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa     3780

uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        3868


<210>  3509
<211>  4936
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3509
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcacuguu caacaagugc      240

auuauaauca gcauuaaucu cgacuuuucc aacaaagaga aguguaugac caagccauac      300

ucuaucggac uugauauugg uacaaauuca guuggcuggg ccgugaucac ugacaacuau      360

aaaguaccga guaagaaaau gaaggugcug gggaauacga gcaaaaagua cauaaagaaa      420

aacuuguuag gaguccugcu auucgauucc ggcaucaccg cggaagguag gcggcucaag      480

cgcacagcuc gacguagaua uacuaggcgg cgcaauagga uucuguaccu ucaggagauc      540

uuuucuaccg aaauggccac acuggacgau gcauucuuuc aaagauugga cgauucguuc      600

cucgugcccg acgauaaacg ggacucaaag uauccuauuu uugggaaccu gguugaggaa      660

aaaguguacc augaugaguu cccaaccauc uaucaucugc gcaaguaccu cgccgacagc      720

acuaaaaagg cugauuuacg acuggucuac cuugcauugg cccacaugau aaaguauaga      780

ggacacuuuc ugauugaggg cgaguucaau aguaaaaaca augacaucca gaagaacuuu      840

caggacuucc uagauacaua caaugcuauu uuugaguccg accucucucu ggaaaacagc      900

aaacagcuug aggagaucgu gaaggauaaa aucuccaagc uggaaaagaa agacaggauu      960

uugaaacucu uccccggcga gaaaaauuca gggauauuca gugaauuucu gaaguuaauc     1020

guaggaaacc aagccgauuu ccggaaaugc uuuaaccugg acgagaaggc gagccuccau     1080

uucucuaagg aauccuauga ugaggaccuu gagacccugu uggguuacau uggcgaugac     1140

uauucagaug uuuuucuaaa agcaaagaaa cuguacgacg ccauccuccu gagcggguuc     1200

cuuacgguga cugacaauga aaccgaggcu ccucugaguu cugccaugau aaagcguuau     1260

aacgaacaca aagaggauuu ggcacuccug aaggaauaca uucgcaauau cuccuuaaag     1320

acauacaacg aggucuuuaa agacgauacu aagaauggau augcuggcua caucgacggu     1380

aaaaccaacc aggaggauuu cuauguguac cugaagaauc uacucgccga auuugagggg     1440

gcggacuauu uccuggaaaa gauugauaga gaggacuuuc uuaggaagca gcggacauuc     1500

gauaacggaa gcaucccaua ccagauucac uugcaagaaa ugcgagccau acuggacaaa     1560

caggcaaagu uuuaucccuu ccucgcuaag aauaaagagc gcaucgagaa gauucugacc     1620

uucagaaucc cuuacuacgu gggcccacuu gccaggggca acucggauuu ugcuuggucu     1680

auccggaaac gcaaugaaaa gauuacgccg uggaacuucg aggacgucau agauaaagaa     1740

uccucagccg aggcauuuau caauagaaug acaaguuucg accuguauuu gcccgaggaa     1800

aagguucucc cuaagcauag ccuguuauac gagacuuuua acguguauaa ugaacugacc     1860

aaaguaaggu ucauugccga guccaugcgu gacuaccagu uucuagauuc uaagcagaaa     1920

aaggacaucg ugcggcucua uuucaaagau aagcgaaagg ucacugacaa agauauuauc     1980

gaauaccugc acgcuauaua uggguacgac ggaaucgagc uuaaggguau ugagaaacaa     2040

uuuaacagcu cauugaguac auaccaugau cugcucaaca ucauuaauga caaggaguuc     2100

cuggaugacu ccagcaacga agcgaucaua gaagagauua uccacaccuu aacgauuuuu     2160

gaagauagag agaugaucaa acagaggcug ucuaaguucg agaauauauu ugacaaguca     2220

gugcuuaaaa aguugagucg ccggcacuau accggcuggg ggaaacucag cgcaaagcug     2280

aucaacggaa uuagagauga aaaauccggc aauacaaucc uagacuaccu gauugacgau     2340

gguaucucua acaggaauuu caugcagcuc auacaugacg augcccuguc guucaagaaa     2400

aagauucaaa aggcucagau caucggggac gaggauaaag gcaacauuaa ggaaguugug     2460

aaaagccuuc caggaucccc cgccaucaag aaaggcauuu ugcagucaau aaagaucguc     2520

gacgagcugg ugaagguaau gggagggcgc aaaccugaaa guauuguugu ggagauggca     2580

cgagagaauc aguauacuaa ccaagguaag ucuaauagcc agcagcgguu aaaacgucuc     2640

gaaaaguccc ugaaagagcu uggcucaaag auccugaagg aaaacauucc agccaaauug     2700

agcaagaucg auaauaacgc ucuccagaau gacagacugu accuauauua ccugcaaaac     2760

gggaaagaua uguauaccgg agacgaucuc gacauagaca ggcugucuaa cuacgauauc     2820

gaccacauua ucccccaggc cuuucuuaag gauaauucca uugacaacaa agucuuggug     2880

aguagcgcau cuaaucgcgg caaguccgau gacuucccgu cacuggaggu cgugaagaaa     2940

cggaagacau uuugguacca guuacucaaa aguaagcuga ucagccagag aaaauucgau     3000

aaccuuacua aggcggagag gggugggcug cuaccugaag acaaggcugg cuuuauacaa     3060

cgacagcucg uugagacccg ccagauuaca aaacaugugg cccggcuguu ggaugaaaag     3120

uucaauaaca aaaaggacga gaauaacaga gcaguaagga ccgucaaaau caucacucug     3180

aagucgacgu uaguguccca guuucguaaa gauuucgaac ucuauaaggu ucgcgagauu     3240

aaugacuuuc accaugccca cgaugcuuac cugaacgccg ugauagcauc ugcccuucug     3300

aagaaauauc ccaaguugga gccagaguuc gucuacggag acuauccuaa auacaauagc     3360

uuucgggaaa gaaagucagc uacagaaaaa guguauuucu acaguaacau caugaauauu     3420

uucaagaagu ccaucucucu cgcggacggc aggguaauug agcgaccccu gaucgaagug     3480

aacgaggaga ccggggaaag cguuuggaau aaagaguccg aucuagccac uguccggcgc     3540

gugcugucau acccacaagu gaacgucguu aagaaagugg aagaacagaa ccacggacuu     3600

gacagaggua agcccaaagg ccucuuuaau gcuaaccuga gcaguaagcc uaagccaaau     3660

ucuaacgaaa auuugguggg ggcaaaagag uaucuggauc cgaagaaaua cggaggcuau     3720

gccgguauau ccaacagcuu cgcuguauua gucaagggga ccaucgagaa aggcgccaag     3780

aagaaaauua caaaugugcu cgaauuucag ggaaucucga uucuggacag gaucaacuac     3840

cggaaggaua aacuuaauuu ccuguuggag aagggcuaua aagacauaga acucauuauc     3900

gagcugccca aguacucacu auuugagcug ucugauggga gucgaagaau gcuugcaucc     3960

auccucagca ccaacaauaa acgcggagaa auucauaagg gcaaccaaau cuuccugucu     4020

cagaaguuug uuaaauuguu auaucacgcg aagaggauaa gcaauacuau uaacgagaau     4080

caucguaaau acguggaaaa ccacaagaaa gaguucgagg aacuguuuua cuauauccuc     4140

gaguucaaug aaaacuacgu cggugccaag aagaacggga aacugcuuaa uuccgcuuuu     4200

cagucauggc agaaccacag uauugacgag cuguguagcu cuuucaucgg accuacaggc     4260

uccgaacgga aggguuuguu ugagcuaacg ucaagaggca gugccgcaga uuucgaguuc     4320

cuggggguga aaauaccaag guaucgcgac uacaccccca gcucccuccu gaaggaugcu     4380

acucuuauuc aucaaucugu uacaggauua uaugaaaccc gaaucgaccu ggccaaacuc     4440

ggcgagggga agagaccugc agccacaaag aaagcuggac aggcgaagaa aaagaaauaa     4500

ggacuaguga agggaacacc caaauuuaau ucagccuuaa gcacaauuaa uuaagaguga     4560

aacguaauug uacaagcagu uggucaccca ccauagggca ugaucaacac cgcaaccuuu     4620

ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu cagcuugcuu agauguucca     4680

aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa aaaaaggcca caaaaguucc     4740

cucucacuuu caguaaauaa aauaaaagca gcaacagaaa uaaagaaaua aaugaaauuc     4800

aaaaugaaau aaauauugug uugugcagca uuaaaaaauc aauaaaaauu aaaaaugagc     4860

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4920

aaaaaaaaaa agaauu                                                     4936


<210>  3510
<211>  4012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3510
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcauggua cgccagccug      240

auguccgcgc aucaccucag gguuggaauu gacgugggua cccauucugu aggccuugcu      300

acacugcggg uggaugacca cgggacucca auagaguugu uaucagcccu gagucauauc      360

cacgauagcg gagucggcaa ggaagguaaa aaggaccacg auacgcgcaa gaaacuaucc      420

gggauugcac gacgugccag aaggcuccug caucaccggc gcacccagcu ucaacagcug      480

gacgaggugu ugagagaucu cggauucccg auccccacac cuggcgaauu ucuggaccug      540

aacgagcaga cugauccaua uaggguuugg cgggugcgcg cucgacucgu cgaagagaag      600

uuacccgagg aacugagagg cccugcaauu ucuauggccg ugaggcauau cgcucggcac      660

cguggguggc gcaauccaua cucgaaagua gagucacuuu ugagccccgc cgaagagagu      720

ccuuucauga aggcgcugag agagaggaua cuagcaacca caggagaagu ucucgacgau      780

gguaucaccc caggccaggc cauggcucaa guggcccuga cucacaacau uuccaugcgg      840

gggccggagg gaauccuugg caaacugcau cagucugaca augcaaacga aauucgaaag      900

aucugcgcuc gccagggugu cagccccgau guguguaagc aguugcucag agccguguuu      960

aaagcggacu ccccuagggg gucagccguc agucggguug caccagaccc ccugccugga     1020

caaggcagcu uccgcagagc uccaaagugc gaucccgagu uucagagguu ccguauaauu     1080

ucuaucgugg ccaauuuacg gauuuccgaa acaaaaggcg agaaccgacc gcugaccgcu     1140

gacgagagaa ggcacguagu gacguuucuc acugaagauu cacaggccga ccuuaccugg     1200

gucgaugugg cagagaagcu ggggguucau cgccgggacu ugagaggaac agccgugcac     1260

acugaugacg gugaaaggag cgcugcgcgc ccucccaucg augcaaccga ccgaaucaug     1320

cggcagacaa aaauuaguuc ucuaaagacc uggugggagg aagccgauuc cgagcaacgu     1380

ggcgcuauga uaagauaucu guacgaggac ccaacggaca gcgaaugugc cgagaucauu     1440

gcagaacucc cugaggaaga ucaggccaaa cuggacucgc uucaucugcc cgcugggagg     1500

gccgcauauu cucgcgaguc cuugacugcg cucucagauc acaugcuggc uacaaccgac     1560

gauuuacacg aggcccggaa gagacuguuc ggagucgacg auaguugggc accacccgcc     1620

gaagcuauca augccccugu gggcaaccca agcguagaca ggacucuaaa gauaguuggu     1680

cgauaccucu ccgcagugga gucuaugugg gggacaccgg aagucauuca uguggagcac     1740

guccgcgaug gcuuuaccag cgagcggaug gcugacgaaa gagauaaagc caauaggcgu     1800

cgcuauaacg acaaucagga ggcgaugaag aaaauccaac gggauuacgg aaaggaaggc     1860

uacaucucaa gaggggacau ugugaggcug gacgcccuug aguugcaggg augcgcuugu     1920

cuguauugcg guacgaccau cggcuaccau acaugucagc ucgaucacau uguuccccag     1980

gcagggccug gaaguaacaa ucgacggggc aaccuggugg ccguaugcga acgcuguaau     2040

agauccaaaa gcaacacucc auucgcuguc ugggcccaaa agugcgguau accccacgug     2100

gggguuaagg aggcaaucgg cagggugcgg ggauggcgaa aacagacccc uaacacaucu     2160

ucagaggacc uuacuagacu gaagaaagaa gucauugcgc gcuugaggcg uacccaggag     2220

gauccagaaa ucgacgagcg gaguauggaa agcguggccu ggauggcuaa ugagcuccau     2280

cacagaaucg ccgcagcuua ucccgagaca accguaaugg uguacagggg cuccauuacu     2340

gccgcagccc gcaaggcugc ggggauagau ucucgaauca accugauugg agaaaaaggu     2400

agaaaggacc ggaucgauag gcgccaucac gccguugacg caucggucgu ggcuuuaaug     2460

gaggccagcg uggccaagac gcuggcugaa cguuccucac uaagaggcga gcagcggcuc     2520

acagggaaag aacaaaccug gaagcaguau acuggaagua ccgucggcgc aagggagcau     2580

uuugagaugu ggcgcgguca caugcugcac cuuacagaau uguucaauga gcgacuggcc     2640

gaagauaaag uuuacgugac ccagaacauu agacuccggc ugucugacgg gaaugcgcau     2700

acugugaacc cgagcaaguu aguaucccac aggcugggcg auggacuuac aguccagcaa     2760

aucgaccgcg cuuguacgcc ugccuugugg ugcgcacuca ccagagagaa agauuuugac     2820

gagaagaaug gccugccagc cagggaagac cgggcuauac gugugcaugg gcacgagauc     2880

aagucaagcg auuauauuca gguuuucucu aaacgcaaga aaacugacuc cgaucgagac     2940

gaaacacccu ucggagcaau cgccgugaga ggcgguuuug ucgagauugg gccuaguauc     3000

caucacgcga ggauauaccg gguggaagga aagaaacccg uuuacgcuau gcuacgcgug     3060

uucacccacg aucugcucag ccagagacau ggcgaccugu uuucugccgu aauuccaccu     3120

caguccaucu caaugaggug cgcagagccc aaacuucgga aagccauuac aacugguaac     3180

gcuaccuauu ugggcugggu cguggugggg gaugagcugg aaaucaaugu cgacaguuuc     3240

acgaaguacg ccauaggacg auuuuuagag gauuucccaa acaccacaag auggcgcauc     3300

uguggcuaug acacuaauag caagcucacc cugaaaccga uuguucuugc agcugaaggg     3360

cuggagaacc cuucguccgc cgugaaugaa aucguagagu ugaagggaug gaggguggcg     3420

auuaacgucc ucacaaaagu gcaccccacu guugugcggc gugaugcccu gggcagacca     3480

agguacucua gccgcucaaa ucuacccacc aguuggacga ucgagaagcg gccugcagcu     3540

acaaagaagg ccggucaagc uaagaaaaag aaauaaggac uagugaaggg aacacccaaa     3600

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu     3660

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa     3720

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu     3780

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     3840

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     3900

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     3960

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             4012


<210>  3511
<211>  4873
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3511
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaccaa gccauacagc      240

auuggacugg acauagguac aaacuccguu ggcugggccg ugacuacgga uaauuauaaa      300

guaccgucua agaagaugaa agugcucggg aacaccucaa agaaauacau caagaaaaau      360

cuucugggag ucuuguuauu cgacaguggc auuacagcgg aggguaggcg gcugaagcgc      420

acugcucgac guagauauac caggcggcgc aacagaaucc uauaccucca ggaaauuuuu      480

agcacagaga uggccacccu ggaugacgca uucuuucaaa ggcuugauga cuccuuccug      540

gugcccgaug acaagcggga uucuaaauau ccuaucuuug ggaauuuggu ugaagagaag      600

gccuaccaug acgaguuccc aacuauauau caccuccgca aauaccuggc ugauucgaca      660

aagaaagcag accugcgacu cguguacuua gcccuggcuc auaugaucaa guauagagga      720

cacuuucuua uugagggcga guucaacuca aagaauaacg auauccagaa gaauuuucag      780

gacuucuugg acaccuacaa cgccauuuuu gaaagcgauc ugagucuaga aaauuccaag      840

cagcucgaag agaucgucaa agacaagaua ucuaaacugg agaagaaaga uaggauucuu      900

aagcuguucc ccggcgaaaa gaacagcggg aucuuuuccg aguucuugaa acucauugug      960

ggaaaucaag cggacuuccg gaagugcuuu aaccuggaug aaaaagcauc auuacauuuc     1020

aguaaggaga gcuaugacga agaucuggag acgcuccuug guuacaucgg cgacgauuau     1080

ucugacguau uucugaaagc caagaaguug uacgaugcua uccuacuguc cggguuccuc     1140

acuguuaccg acaaugagac agaagccccu cugucaagcg caaugauuaa acguuauaac     1200

gagcacaagg aagaccuugc ucuguugaaa gaguacauac gcaacaucag ucucaagacu     1260

uacaaugaag uguuuaaaga ugacaccaag aacggauaug ccggcuacau ugaugguaag     1320

acaaaucagg aggacuucua ugucuaccug aaaaaguuac uggcggaguu ugaaggggcc     1380

gauuauuucc uagagaaaau cgacagagaa gauuuucuca ggaagcagcg gaccuucgac     1440

aacggaucua uaccauacca gauucaccuu caagagaugc gagcaauccu ugauaaacag     1500

gcuaaguuuu aucccuucuu ggccaaaaau aaggaacgca ucgagaagau ucugacguuu     1560

agaaucccuu acuacguggg cccacucgcu aggggcaacu ccgacuucgc cuggagcauu     1620

cggaaacgca augagaagau aacuccgugg aacuucgaag augugaucga caaagagucg     1680

ucugcagaag ccuuuauuaa uagaaugaca uccuucgauc uguaucuucc cgaggagaag     1740

guccugccua aacauucauu gcucuacgaa accuuuaacg uuuauaauga gcugacuaag     1800

gugagguuca ucgcugaaag uaugcgugac uaccaguuuu uagacagcaa gcagaaaaag     1860

gauaucguac ggcuguauuu caaagacaag cgaaaaguga cagauaagga cauuauagag     1920

uaccuacacg cgaucuaugg guacgaugga auugaacuca aggguaucga gaaacaauuu     1980

aacuccucuc ugagcaccua ccaugaccuu uugaauauua ucaacgauaa ggaguuccug     2040

gacgauucaa guaaugaagc aauaaucgag gaaauuaucc acacgcucac cauuuuugag     2100

gacagagaaa ugaucaaaca gaggcugucc aaguucgaga acauauuuga uaaaagcguc     2160

uuaaagaagc ugucucgccg gcacuauaca ggcuggggga aacuuucagc caaguugauu     2220

aacggaauca gagacgagaa aaguggcaau acuauucucg auuaccugau cgacgacggu     2280

auaagcaaca ggaauuucau gcagcuaauc caugaugacg cucuguccuu uaagaaaaag     2340

auucaaaaag cccagaucau uggggaugaa gacaagggca acaucaagga ggugguuaaa     2400

ucucucccag gaucgcccgc aauaaagaaa ggcauucugc agagcaucaa gaucguggau     2460

gaacuuguca aagugauggg agggcgcaag ccugagucca uuguaguuga aauggcccga     2520

gagaaucagu auaccaacca agguaaguca aauagucagc agcgguugaa acgucuggag     2580

aagucuuuaa aagaacucgg cagcaagauc cugaaagaga acauuccagc uaagcugucc     2640

aagauagaca auaacgcccu gcagaaugau agauuguacc ucuauuaccu gcaaaacggg     2700

aaagacaugu auacaggaga ugaccuagau aucgacaggc ugucaaauua cgacauugau     2760

cacaucauuc cccaggcauu ccucaaggac aacagcaucg auaauaaagu gcuggucucu     2820

uccgcgagua accgcggcaa gagcgacgau gugccgucuc uugaagucgu gaaaaagcgg     2880

aagacuuucu gguaccaguu gcugaaaucc aaguuaauau cacagagaaa auuugacaac     2940

cucaccaagg cugagagggg ugggcugagu ccugaggaua aagccggcuu cauccaacga     3000

cagcuuguug aaacacgcca gauuaccaag cauguggcac ggcugcuaga cgagaaguuu     3060

aauaacaaaa aggaugaaaa uaacagagcc guaaggacug ucaaaaucau uacgcucaag     3120

agcacacugg ugucgcaguu ccguaaagac uuugaguugu auaagguucg cgaaaucaau     3180

gauuuccacc augcucacga cgccuaccug aacgcagugg ucgcuuccgc cuuacucaaa     3240

aaguauccca agcuggagcc agaguuugug uacggagauu auccuaaaua caauucuuuc     3300

cgggaaagaa agagcgcgac cgagaaagua uauuuuuacu caaacauaau gaauauuuuc     3360

aagaaaagua ucucccuugc cgacggcagg gugaucgaac gaccccugau ugagguuaac     3420

gaagagacug gggagucugu cuggaauaag gaaagcgacu uggcuaccgu gcggcgcgug     3480

cucuccuacc cacaagucaa cguugugaag aaaguggagg aacagaauca cggacuggau     3540

agagguaagc ccaaaggccu auuuaacgca aaucugucaa gcaagccuaa accaaacagu     3600

aacgagaauc uuguaggggc caaggaauau cucgacccga agaaauacgg aggcuaugcu     3660

gguauaucua acuccuucac aguccuggug aaggggacca ucgagaaagg cgccaagaaa     3720

aagauuacua auguuuugga guuccaggga aucagcauuc uggauaggau caacuaccgg     3780

aaggacaaau uaaauuuucu ccuggaaaag ggcuauaaag auauagagcu uaucauugaa     3840

cugcccaagu acucguuguu cgagcucuca gacgggucuc gaagaaugcu ggcaaguauc     3900

cuauccacaa acaauaaacg cggagagauu cauaagggca accaaaucuu ucugagccag     3960

aaauucguga aacuucucua ucacgcgaag aggauaucua auacgauuaa cgaaaaucau     4020

cguaaauacg ucgagaacca caagaaagaa uuugaggaac uguucuacua uaucuuggag     4080

uuuaaugaga acuacguggg ugccaagaaa aaugggaagu uacugaacag cgcuuuccag     4140

uccuggcaga aucacucaau cgaugaacuc uguaguagcu uuauuggacc uaccggcucu     4200

gagcggaagg gucuguucga acuuacuucc agaggcucag ccgcagacuu ugaguuccug     4260

gggguuaaaa ucccaaggua ucgcgauuac acacccagua gcuugcuaaa ggacgcuacc     4320

cugauacauc aauccgugac aggacucuau gaaacucgaa uugaucuggc caaacuuggc     4380

gaggggaaga gaccugcagc caccaaaaag gcuggacagg cgaagaaaaa gaaauaagga     4440

cuagugaagg gaacacccaa auuuaauuca gccuuaagca caauuaauua agagugaaac     4500

guaauuguac aagcaguugg ucacccacca uagggcauga ucaacaccgc aaccuuuccu     4560

uuuuccccca gugauucuga aaaaccccuc uucccuucag cuugcuuaga uguuccaaau     4620

uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa aaggccacaa aaguucccuc     4680

ucacuuucag uaaauaaaau aaaagcagca acagaaauaa agaaauaaau gaaauucaaa     4740

augaaauaaa uauuguguug ugcagcauua aaaaaucaau aaaaauuaaa aaugagcaag     4800

aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4860

aaaaaaaaga auu                                                        4873


<210>  3512
<211>  3955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3512
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagccgc guucaagcca      240

aacagcauua auuacauacu gggacucgac aucgguauug cuuccguugg cugggccaug      300

guggagaucg augaagagga aaacccgauu aggcuuaucg accugggggu acggguguuu      360

gagcgcgcag aaguccccaa aaccggagau ucuuuggcca uggcucgacg uuuagcaaga      420

ucagugaggc ggcugacacg cagaagggcc caucggcuac uccgcacucg aagacugcuu      480

aagagggagg gcguucugca ggcugccaau uucgacgaga acgguuugau aaagagucuc      540

ccuaauacgc cauggcaacu gcgggcggca gcccuggauc guaaacucac ccccuuagaa      600

uggagcgcug ugcugcuuca cuugaucaag caucgcgggu aucuguccca gaggaaaaac      660

gagggagaaa cagccgacaa ggagcuaggc gcacuccuga aaggcgucgc ugggaaugcc      720

cacgcgcuuc agacuggaga uuuuaggacc ccugccgagc uggcauugaa caaguucgaa      780

aaggagucug gucauauucg gaaucagcga ucggacuacu cacacacauu uagccgcaaa      840

gaucuccaag cugaacugau cuuacuguuc gagaagcaga aagaauuugg caacccacac      900

gugagugggg gacucaagga gggcauugag acccuucuga ugacucagag acccgccuug      960

uccggugacg cuguacagaa aaugcuaggg cauugcacau ucgaaccugc cgagccaaag     1020

gcagccaaaa auaccuauac ggcugaaagg uuuaucuggc ugacuaagcu caacaaucug     1080

cggauacuug agcaaggauc ugaacgcccg cugaccgaua cagagagagc gacuuugaug     1140

gacgagcccu acagaaagag caaacucacc uaugcacagg cccguaagcu guuaggccug     1200

gaagauacag cuuucuuuaa aggccuacgg uacgggaagg acaacgccga ggcauccacc     1260

cucauggaaa ugaaagccua ucacgcuauu ucacgagccc uggagaagga aggacuuaag     1320

gacaaaaaga guccuuugaa ucugagccca gagcuccagg augagaucgg uacggcauuc     1380

ucucuguuua aaacugacga agauauuaca ggcagacuua aggacaggau ccagcccgag     1440

auccuggaag cguugcucaa acauauuucc uucgauaagu ucguucaaau aucacugaag     1500

gcuuuacgcc ggaucgugcc ucugauggag caggggaaaa gauacgacga ggccugugca     1560

gaaauuuacg gagaucacua uggcaagaaa aacaccgagg aaaagaucua ccuaccaccc     1620

auaccggccg acgagauuag gaacccuguc gugcuccgcg cucugagcca ggcccgaaaa     1680

gugaucaaug gugucguucg gcguuauggg agucccgcaa gaauccacau ugaaacugcu     1740

agggaggugg gcaagucuuu uaaggaucgc aaagagaucg aaaagcggca agaggaaaac     1800

agaaaagaca gggagaaggc cgcggccaaa uuccgagaau acuuuccaaa uuucguagga     1860

gagccuaagu ccaaagauau ucuuaaguug cgccuguaug agcagcagca uggcaagugc     1920

cucuacagcg ggaaagaaau aaaccuggga cgguuaaaug agaaggguua uguggaaauc     1980

gaccacgcuc ugcccuuuuc gagaacaugg gacgauucuu ucaacaauaa aguccuugug     2040

uugggcuccg agaaccagaa uaaggggaac caaaccccau acgaauacuu uaauggaaaa     2100

gacaacucaa gggaguggca ggaguucaag gcacguguug aaacgagucg cuuuccccgg     2160

agcaagaaac agaggauucu ccugcagaag uucgaugagg acggcuuuaa agaaagaaau     2220

cuaaacgaua cccgauaugu gaaucgguuc cugugucaau uugucgccga ccgcaugaga     2280

cucacaggua aggggaaaaa gaggguguuc gcuuccaacg gccagaucac uaaccugcuu     2340

cggggauucu ggggcuugcg aaagguaaga gccgagaaug aucgccauca cgcacuggac     2400

gcgguugugg ucgccugcuc uaccguggcu augcagcaga aaaucacaag guuuguccgu     2460

uacaaggaga ugaacgccuu cgaugggaaa acuauugaca aggaaaccgg agagguguua     2520

caucagaaaa cacacuuucc ucagccaugg gaguucuuug cacaggaagu uaugauacgg     2580

guguucggua agccggaugg caagcccgaa uuugaggagg cugacacccu cgaaaaacug     2640

agaacucuuc uggccgagaa guugagcuca aggccugaag caguacacga guaugucacg     2700

ccacucuucg ugagucgcgc ccccaaucga aaaauguccg ggcagggaca uauggaaaca     2760

guuaagagcg cuaaaagacu ggaugagggc gugucugucc uacgggugcc ucugacccaa     2820

cucaagcuga aagaccuuga gaagauggua aacagggaac gcgagccaaa guuguacgaa     2880

gcgcugaaag cccguuuaga ggcacacaag gacgaucccg cuaaagccuu ugccgaaccg     2940

uucuauaagu acgacaaagc ugguaauaga acucaacagg ugaaggcagu ucgggucgag     3000

caagugcaga agaccggggu gugggucagg aaccauaaug gcaucgccga uaacgcgaca     3060

augguucgcg uggacguguu ugagaaagga gauaaguauu accucguacc uauuuacuca     3120

uggcaggucg cuaaaggcau ccugccagac cgagccgugg uucaggggaa ggaugaagag     3180

gacuggcaac uuauugauga caguuucaau uuuaaauuca gccugcaccc caacgaucua     3240

guggaaguca ucaccaagaa ggcaagaaug uucggauauu uugccuccug ucaccggggc     3300

acugguaaua uaaacaucag gauucaugac cucgaucaca aaaucgggaa gaauggcauu     3360

cuggagggaa ucggugugaa aacagcuuug ucuuuccaga aguaccagau agacgagcug     3420

ggcaaagaaa uucgcccuug cagauuaaag aagaggcccc caguucggaa acguccugca     3480

gccacgaaga aagcggggca ggcuaagaaa aagaaguaag gacuagugaa gggaacaccc     3540

aaauuuaauu cagccuuaag cacaauuaau uaagagugaa acguaauugu acaagcaguu     3600

ggucacccac cauagggcau gaucaacacc gcaaccuuuc cuuuuucccc cagugauucu     3660

gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa auuuaguaag cuuaaggcgg     3720

ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc ucucacuuuc aguaaauaaa     3780

auaaaagcag caacagaaau aaagaaauaa augaaauuca aaaugaaaua aauauugugu     3840

ugugcagcau uaaaaaauca auaaaaauua aaaaugagca agaucuaaaa aaaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu          3955


<210>  3513
<211>  4711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3513
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagaa accauacacc      240

auuggacugg acauagguac aaacagcguu ggcugggccg ugcucacuga ucaguaugac      300

cuuguaaaga ggaagaugaa aaucgcgggg gauuccgaga agaaacaaau uaagaaaaau      360

uucuggggag ugcggcuguu ugacgaaggc cagacggcug ccgaucgccg aauggcacgu      420

accgccagaa ggcggaucga gcgcagaagg aaccggauuu cuuacuugca ggguaucuuc      480

gcugaagaga ugucaaagac agacgcaaac uuuuucugcc gcuuaaguga uagcuuuuau      540

gucgacaaug aaaagcgaaa uuccagacau ccguucuuug ccacuauaga ggaggaagug      600

gaguaccaca aaaacuaucc caccaucuac caucugaggg aggagcuagu uaauucuucg      660

gaaaaggcug aucuccggcu gguguaucuu gcccuggcgc acauuaucaa auaccguggg      720

aacuucuuga uugaaggagc acucgacaca cagaauaccu cagucgaugg caucuacaag      780

caauuuauac agacuuauaa ccagguguuc gccagcggca uugaggacgg gagucugaaa      840

aagcuggaag auaauaaaga cguagcuaag auccucguug agaaggugac acgcaaagaa      900

aaguuagaga ggauucugaa acuuuacccu ggagagaagu ccgccgguau guuugcacag      960

uucaucucuu ugaucgucgg cagcaaaggg aacuuucaaa agccauucga ccugauugaa     1020

aaguccgaua uagagugugc uaaagacuca uaugaagagg aucuagaaag ucuccuggcc     1080

cuuaucggag acgaguacgc ggagcuguuc guggccgcaa agaaugcuua uagcgccgug     1140

gucuugucuu ccauuaucac cguugcugaa acggagacua acgccaaacu cucagcaagc     1200

augauagaaa gguuugauac ccaugaggaa gaccugggcg aguuaaaggc cuucauuaaa     1260

cugcaccucc ccaagcacua cgaggaaauc uuuaguaaua cagagaagca ugguuaugcu     1320

ggguacaucg auggaaaaac uaagcaggcg gacuucuaca aauauaugaa gaugacccuu     1380

gaaaacauug agggcgcaga uuacuuuauc gccaaaauug agaaggaaaa cuuccugcgg     1440

aagcagcgaa cauuugacaa uggcgcuaua ccucaccagu ugcaucuaga ggaacuggag     1500

gccauccucc accaacaggc aaaauauuac ccauuccuga aggaaaacua ugauaaaauu     1560

aagucucuug ugaccuuucg cauccccuac uucguagggc cucuggccaa uggacagucc     1620

gaguuugcuu gguugacgag aaaagccgac ggugagauca ggccauggaa cauugaagag     1680

aagguggacu ucggcaaaag cgcagucgau uuuauagaaa agaugacuaa uaaggacaca     1740

uaucucccga aagagaacgu gcugcccaag cacucguuau gcuaccaaaa auaccugguu     1800

uauaaugaac uaaccaaggu gcgguacauc aacgaucagg ggaaaacuuc uuauuucucc     1860

ggacaggaga aggagcagau uuucaaugac cucuuuaagc aaaaacgcaa ggucaaaaag     1920

aaagaucugg aacuuuucuu gagaaacaug ucacaugugg agaguccuac aaucgaaggc     1980

cuggaggaca gcuuuaauuc cucuuacagc accuaucacg aucuccugaa gguagguauu     2040

aagcaggaaa uccuugacaa cccaguuaau acggagaugc uggagaacau agugaaaauc     2100

uugaccgucu ucgaagauaa gaggaugauu aaagagcagc uccagcaauu uucagacgug     2160

cuggaugggg ucguguuaaa gaaacuggaa cgucggcauu acacaggcug gggacgacua     2220

agugcgaagc uccugauggg caucagagac aagcaguccc accuuacuau uuuggauuau     2280

cugaugaaug acgacgggcu caacaggaac cugaugcagu uaaucaauga uagcaaccug     2340

ucuuucaaau caauaauuga gaaggagcag guuaccacag cugacaaaga uauccaaagu     2400

auuguggccg accuugcagg aagccccgcc aucaagaaag guauauugca gucccucaag     2460

aucguagaug aacuggucuc ugugaugggc uacccuccac agacuauugu uguggagaug     2520

gcucgcgaaa aucagaccac agggaaagga aagaacaauu cgcggcccag auacaagagc     2580

cuagagaaag ccaucaagga auuuggcucc caaauucuga aagagcaucc gaccgacaac     2640

caggagcuca ggaauaaccg ccuguaucuu uacuauuugc agaaugguaa ggauauguac     2700

acugggcaag accuggauau ccacaacuua ucaaauuaug acauagauca cauugucccu     2760

cagaguuuca ucacggacaa cucuaucgau aaucucgugc ugacaagcuc cgcaggcaac     2820

cgagaaaaag gagacgacgu acccccacuu gagauugugc ggaagcguaa ggucuuuugg     2880

gaaaaacugu accagggcaa uuugauguca aagagaaaau ucgauuaucu caccaaggcu     2940

gagagagggg gacugacuga agccgacaaa gcgcgcuuua uccaucggca gcuaguugag     3000

accagacaaa uuacaaagaa cguggccaac auacugcacc agagguucaa uuacgagaag     3060

gaugaccaug guaacaccau gaaacaggug cgaaucguca cucucaagag cgcucugguu     3120

ucucaguuuc gcaaacaauu ccagcuuuac aaggugcggg augugaauga cuaucaccac     3180

gcacaugaug ccuacuugaa cggcguaguc gcuaauacac uguuaaaagu guauccucag     3240

cucgaacccg aguucguuua cggggacuau caccaguuug auugguucaa ggccaacaag     3300

gcaacggcga aaaagcaauu uuacaccaau auuaugcugu ucuuugccca gaaagacaga     3360

aucauugaug aaaacggaga gauccuuugg gacaagaaau aucuggacac ugugaagaag     3420

gucauguccu acaggcagau gaauauagug aaaaagacag aaauccaaaa aggcgaguuc     3480

aguaaggcua ccauuaaacc aaaggguaac agcucuaaac uaaucccccg uaagacaaau     3540

ugggauccua ugaaguacgg gggccucgac uccccaaaca uggccuaugc aguugugauu     3600

gaguacgcua aaggaaagaa uaaacuggua uuugaaaaga aaaucauacg cgucacuauu     3660

auggagcgga aggccuucga aaaggaugag aaagcauuuu uggaggaaca gggcuauaga     3720

cagccgaagg ugcuggccaa auuacccaag uacacccucu augaguguga agaggggagg     3780

cgacggaugc uggcuucagc gaacgaagcc cagaaaggaa aucaacaggu gcuuccuaac     3840

caucugguca cguugcucca ccaugcagcu aacugcgagg uuagugacgg caagagccug     3900

gauuacaucg agucgaaucg cgaaauguuc gccgagcuac uggcccacgu guccgaauuu     3960

gcuaagagau auacccuugc agaggccaac cucaauaaaa ucaaccagcu guucgaacag     4020

aauaaggagg gugacauuaa agcgauagcu caaucuuucg uagauuugau ggccuuuaac     4080

gcaauggggg ccccagcuag cuucaaguuu uucgagacaa cuaucgaaag gaaacgguac     4140

aauaaccuga aggaguuacu caauucaacc auuaucuacc agaguauuac aggacuguau     4200

gaaucccgaa agagacuuga cgauaaacgc cccgcagcca cuaagaaagc gggccaggcu     4260

aagaaaaaga aauaaggacu agugaaggga acacccaaau uuaauucagc cuuaagcaca     4320

auuaauuaag agugaaacgu aauuguacaa gcaguugguc acccaccaua gggcaugauc     4380

aacaccgcaa ccuuuccuuu uucccccagu gauucugaaa aaccccucuu cccuucagcu     4440

ugcuuagaug uuccaaauuu aguaagcuua aggcggccua cagaagaaaa agaaaaaaaa     4500

ggccacaaaa guucccucuc acuuucagua aauaaaauaa aagcagcaac agaaauaaag     4560

aaauaaauga aauucaaaau gaaauaaaua uuguguugug cagcauuaaa aaaucaauaa     4620

aaauuaaaaa ugagcaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                                    4711


<210>  3514
<211>  4744
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3514
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagaa accauacagc      240

auuggacugg acauagguac caacuccguu ggcugggccg ugguaacaga ugacuauaag      300

gugccggcga agaaaaugaa gguccucggg aauacugaua aaucucauau cgagaagaac      360

cuucugggag cuuuguuauu cgacucaggc aauacggccg aagauaggcg gcugaaacgc      420

accgcacgac guagauacac aaggcggcgc aaccggauuc uauaucucca ggagaucuuu      480

agugaagaga uggguaaggu ggacgauagc uucuuucaca ggcuggaaga cuccuuccuu      540

guuacugagg auaagagagg ggagcgccau cccauuuuug gaaaucugga agaggaagug      600

aaauaccacg agaacuuccc uaccaucuau cauuugcgac aauaccucgc cgacaaucca      660

gagaaggucg aucugagacu gguguaucuc gcuuuagcac acauaaucaa auuuaggggc      720

cacuuccuga uugaaggcaa guuugacaca cggaacaaug auguacagcg ucuuuuccag      780

gaguuuuugg ccguuuacga caacaccuuc gaaaauucuu cgcugcagga gcaaaacgug      840

caggucgaag agauccuaac ugacaaaauu ucaaagagcg cuaaaaagga ucgcgugcuc      900

aagcuguuuc ccaaugagaa aaguaacggg agauucgccg aguuccuuaa gcugaucgug      960

ggaaaucagg cggacuuuaa aaagcauuuc gaguuggaag agaaagcacc ucuccaguuu     1020

uccaaggaua cauacgaaga ggagcuggaa gucuuacugg cccaaauagg ugacaacuau     1080

gcugaacucu uccuuucugc caagaaacug uacgauagca uuuugcuauc cggcauccug     1140

accguuacgg acguggggac uaaggcacca cucucagcua guaugauuca gagguauaac     1200

gaacaccaga uggaucuggc ccagcuuaaa caauuuaucc ggcagaagcu gagcgacaaa     1260

uacaaugagg uauucucuga uguguccaag gacggauaug cgggcuacau cgaugguaag     1320

accaaccagg aagccuuuua uaaauacuug aaggggcucc ugaauaaaau ugagggauca     1380

ggcuacuucu uagacaagau agagcgagaa gacuuucugc gcaaacaaag aacauucgau     1440

aacggcagca ucccccauca gauucaccua caggagauga gggcaaucau acggcgccag     1500

gcugaauuuu auccuuuccu cgccgacaau caagauagaa uugagaagcu gcuuacuuuu     1560

aggaucccau acuaugucgg gccguuggcu cguggaaaga gugacuucgc cuggcugucu     1620

cggaaauccg cagauaagau cacccccugg aacuucgacg agauugugga uaaagaaagc     1680

ucggccgagg cuuuuaucaa ucgaaugaca aacuacgacc ucuaucugcc uaaucagaag     1740

guucuuccaa aacacucucu guuguacgaa aaguucaccg uguauaacga gcucacgaaa     1800

gucaaguaca agacugaaca ggguaaaaca gcguuuuucg augcaaauau gaagcaggag     1860

auuuuugacg gcguguucaa aguauacaga aagguuacca aagauaagcu gauggacuuu     1920

uuagagaagg aguucgauga auuuaggaua guggaccuga cugggcuaga caaagaaaac     1980

aaggucuuca augccuccua uggaacauac caugaucucu gcaaaauccu ggacaaggau     2040

uuucuugaca acaguaaaaa ugagaagauu uuggaagaua ucgugcugac ccucacgcug     2100

uucgaggacc gcgagaugau ccggaagaga uuagaaaacu auucagaucu gcuuaccaaa     2160

gagcaaguca agaaauugga aaggcgccac uacacaggcu ggggucgacu cagcgcugag     2220

cugauucaug ggauacggaa caaggaaucc cguaaaacua uccuagacua ucugauugau     2280

gacggcaauu cuaacagaaa uuuuaugcag cucaucaacg augacgcccu gagcuucaag     2340

gaggagauug caaaggccca ggugaucgga gaaaccgaua aucuuaacca gguuguguca     2400

gacauagcug gcagucccgc caucaaaaag gggauuuugc aaucccugaa aaucguagac     2460

gaguuaguca agauuauggg acaccagccu gaaaauaucg ugguugagau ggcaagggag     2520

aaccaguuca caaaucaggg ucgccggaac agccaacaga gacucaaagg ccugacugau     2580

ucuauaaagg aauuuggguc acagauucuu aaagagcauc caguggaaaa uagucaacug     2640

cagaacgaca gguuguuccu cuacuaucug cagaauggac gagauaugua caccggcgag     2700

gaacuagaca ucgauuaccu gagccaguau gacauugauc acaucauacc ccaagcguuu     2760

aucaaggaca acuccauuga uaaucgcguc cucacaucuu cgaaggagaa ccgggguaaa     2820

agcgacgaug ugccguccaa ggacguagug agaaaaauga agucauacug gaguaaacug     2880

cuuucugcua aguugaucac ccagaggaag uucgacaacc ugacuaaagc cgagcguggg     2940

ggcuuaacgg augacgauaa ggcaggauuu auuaaacgcc agcucguuga aacacggcag     3000

aucaccaagc acgucgccag aauacuggac gagagguuca auacugaaac cgaugagaac     3060

aauaaaaaga uucgacaagu gaagaucgug acacuuaaaa gcaaccuggu cuccaauuuu     3120

cggaaggagu ucgaacuaua uaaaguucgc gagaucaacg acuaccauca cgcucaugau     3180

gccuaucuca augcagugau uggcaaggcu cuguuggggg uguacccuca gcuggaaccc     3240

gaguuuguau auggagacua cccacacuuc cacggucaua aagaaaacaa ggccaccgcg     3300

aagaaauuuu ucuacucaaa uaucaugaac uucuuuaaga aagaugacgu cagaacugau     3360

aagaauggcg agauuauaug gaaaaaggac gaacacauca gcaacauuaa gaaaguguua     3420

ucuuauccuc agguuaauau cgugaagaaa gucgaggagc agacaggggg auucuccaag     3480

gaaaguauuc uccccaaagg caacagcgau aagcugaucc caaggaaaac gaagaaguuu     3540

uacugggaca ccaaaaagua ugguggguuc gacucuccca uaguggccua cuccauccuu     3600

guuauugcug auaucgagaa aggcaagagu aaaaagcuga agacugugaa agcauuggua     3660

ggagucacaa uuauggaaaa gaugaccuuu gagcgggacc cuguggccuu ccucgagcga     3720

aaaggcuaua gaaaugugca agaagagaac aucauaaagc ugccaaaaua cucacuauuu     3780

aagcuggaaa acgggcgcaa gaggcuucuc gcuagcgccc gugagcugca gaaaggaaau     3840

gaaauugucu ugccgaacca ucugggcaca uuacucuauc acgcaaagaa uauccauaaa     3900

guugaugagc ccaagcaccu ggacuacgug gauaaacaca aggacgaguu caaggaacuu     3960

cuggauguag ugucgaacuu uuccaaaaag uacacuuugg cggaggguaa ucucgagaaa     4020

aucaaggaac uguaugccca gaacaauggg gaagaccuaa aagagcuggc uucuagcuuc     4080

auuaaccuuc ucaccuuuac ggccauagga gcuccugcaa ccuucaaguu uuucgauaaa     4140

aauaucgacc ggaagagaua cacaucaacu accgagauuc ugaacgccac auugauccau     4200

caaaguauua cuggcuuaua ugaaaccagg aucgaucuga auaagcucgg uggcgacaaa     4260

cgcccagcag ccacgaagaa agcugggcag gcgaagaaaa agaaguaagg acuagugaag     4320

ggaacaccca aauuuaauuc agccuuaagc acaauuaauu aagagugaaa cguaauugua     4380

caagcaguug gucacccacc auagggcaug aucaacaccg caaccuuucc uuuuuccccc     4440

agugauucug aaaaaccccu cuucccuuca gcuugcuuag auguuccaaa uuuaguaagc     4500

uuaaggcggc cuacagaaga aaaagaaaaa aaaggccaca aaaguucccu cucacuuuca     4560

guaaauaaaa uaaaagcagc aacagaaaua aagaaauaaa ugaaauucaa aaugaaauaa     4620

auauuguguu gugcagcauu aaaaaaucaa uaaaaauuaa aaaugagcaa gaucuaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag     4740

aauu                                                                  4744


<210>  3515
<211>  3877
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3515
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcacagac aacaaaccug      240

agcuacauuc ucggacuuga ccuggguaua gccuccguug gcugggcggu gguagagauc      300

aaugaaaacg aggauccaau uggguugauc gacgugggag ucaggauuuu cgaacgggcu      360

gaggugccga agacuggcga aucuuuagcc cugucacgcc gacuagcacg uaguacgaga      420

aggcucaucc ggcgcagagc ccauaggcug cuucuggcua aacgguuuuu gaagcgcgag      480

gguauacuca gcaccaucga ucuggagaag gggcugccca aucaagcaug ggaacuccga      540

guugccggau uagagagaag gcuguccgcu auugaauggg gcgccgugcu uuugcaccug      600

aucaaacauc ggggcuaucu aucuaagcgu aaaaacgagu cgcagacaaa uaacaaggag      660

cucggggcgc ugcuuucagg agucgcacag aaucaccagc uguugcaaag cgacgauuac      720

cgcacuccug ccgaacucgc ucugaaaaag uucgccaagg aggaagguca uauuagaaac      780

cagaggggcg cauauaccca cacauuuaau cgguuagacc ugcucgcuga gcuuaaccug      840

uuguucgccc agcagcacca auuugggaau ccacauugca aagaacacau ccagcaguac      900

augaccgagc uacugaugug gcagaagccc gcgcucagug gagaggccau acugaaaaug      960

cuuggcaagu guacucauga gaaaaacgag uucaaggcag cuaaacacac auauuccgcc     1020

gaacgauuug uguggcugac caaguugaau aaccuccgca uucuggagga uggugcugaa     1080

agagccuuaa augaggagga aaggcaacug cuaaucaacc acccuuacga gaagucuaaa     1140

cucacguaug cacagguacg gaagcugcuu ggguugagcg aacaggccau uuucaaacau     1200

cugcgcuacu ccaaggagaa cgcugaauca gcgacuuuua uggagcucaa agcauggcac     1260

gccaucagaa aggcucugga gaaucaagga cuuaaggaca ccuggcagga ucuggccaaa     1320

aagccagacu ugcucgauga aaucggcaca gcauucaguc uguacaaaac ugacgaggau     1380

auucagcagu auuuaaccaa caagguuccc aauagcguga uaaacgcccu gcuagucucu     1440

cucaauuuug acaaauucau cgaacugucc cuuaagucau ugaggaagau ucugccucuc     1500

auggagcaag gcaaacguua cgaccaggcu ugccgggaga ucuaugggca ucacuacgga     1560

gaagccaacc agaagacaag ccagcuguua ccagcaauac cggcgcaaga gauucgaaau     1620

cccguggugc ugagaacccu uagucaggcu aggaaaguca ucaacgccau cauucgccag     1680

uaugguucuc cugcacgggu ucauaucgaa acgggcagag aguuggggaa guccuuuaaa     1740

gaaaggcgcg agauucagaa gcaacaggag gauaaucgaa cuaagcggga aagcgccgug     1800

cagaaauuca aggaacucuu cucggacuuu ucuuccgaac caaaaucaaa ggauauacug     1860

aaauuccguc uauacgagca gcaacacgga aagugucugu auaguggcaa agaaaucaac     1920

auucacagac ucaaugagaa ggguuacgua gagaucgacc augcucugcc cuuuagcagg     1980

acaugggaug acuccuucaa caauaaggug cuugucuugg ccucugaaaa ccagaauaaa     2040

gggaaccaga ccccuuacga guggcugcaa ggcaagauca acagcgaacg cuggaaaaau     2100

uuuguggcau uaguucucgg aucacagugc agugcugcca agaaacagcg gcugcuuacu     2160

caggugauug augacaacaa guucauagau agaaaucuga acgacacaag guauaucgcg     2220

cgauuuuugu ccaauuacau ucaagagaac cuccugcuag ucggcaagaa uaaaaagaac     2280

guguucaccc caaaugggca gaucacggcc cugcuccgca gccggugggg acugauuaaa     2340

gcuagagaaa acaauaacag gcaccaugca cuugaugcca ucguaguugc uugugccacc     2400

cccucuaugc agcagaagau aacacguuuu auccgcuuca aagaggugca cccguauaag     2460

auugagaauc gguacgaaau ggucgaccaa gagucaggug aaaucauuag uccucacuuu     2520

cccgagccau gggcauauuu cagacaggag gugaacauca gggucuuuga caaucauccu     2580

gauacugugu ugaaggaaau gcugcccgac cgaccacagg cgaaccacca guucguucaa     2640

cccuuauucg ugagccgggc cccuacccgc aaaauguccg gccaggggca uauggagaca     2700

auaaagucug cuaaaagacu cgccgaagga auuucgguac ugaggauccc acuuacucag     2760

cugaagccga accuacucga gaauaugguc aacaaagaac gggagcccgc acuguacgcu     2820

ggcuugaagg cccgacuggc agaguuuaau caagauccug ccaaggcuuu cgcgacccca     2880

uuuuauaaac agggugggca acaggugaag gccauuagag uugaacaggu gcagaaaagc     2940

ggcgucuuag ugcgcgagaa caauggagua gcagacaacg cuuccaucgu gaggacagau     3000

guuuucauaa agaauaacaa auuuuuccuc guccccaucu acaccuggca aguggccaag     3060

ggcauucugc cuaauaaagc caucguggcu cacaagaacg aagacgagug ggaagagaug     3120

gaugaggggg caaaguuuaa auucucacuu uuuccaaaug accuggucga auugaagacu     3180

aaaaaggagu acuucuuugg auauuacauu ggucucgauc gugccacggg caacaucagu     3240

cugaaagaac augacgggga gauaucuaag ggaaaggaug gcguuuaucg ggugggugug     3300

aaacuagcgc ugagcuucga aaaguaccag guagacgagc uugggaaaaa uagacagauu     3360

ugcaggcccc agcagcgcca gccgguccga aagagaccug cugccaccaa aaaggcaggc     3420

caagccaaga aaaagaaaua aggacuagug aagggaacac ccaaauuuaa uucagccuua     3480

agcacaauua auuaagagug aaacguaauu guacaagcag uuggucaccc accauagggc     3540

augaucaaca ccgcaaccuu uccuuuuucc cccagugauu cugaaaaacc ccucuucccu     3600

ucagcuugcu uagauguucc aaauuuagua agcuuaaggc ggccuacaga agaaaaagaa     3660

aaaaaaggcc acaaaaguuc ccucucacuu ucaguaaaua aaauaaaagc agcaacagaa     3720

auaaagaaau aaaugaaauu caaaaugaaa uaaauauugu guugugcagc auuaaaaaau     3780

caauaaaaau uaaaaaugag caagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                              3877


<210>  3516
<211>  3955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3516
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagccgc guucaagcca      240

aacccgauua auuacauacu gggacucgac aucgguauug cuagcguugg cugggccaug      300

guggagaucg augaagacga gaaccccauu ugccuuaucg aucugggggu aaggguguuu      360

gaacgggcag aggucccuaa aaccggagac uccuuggcca uggcucgccg auuagcacgu      420

ucugugagaa ggcugacacg gcgcagagcc cauaggcuac uccgggcucg ccgacugcuu      480

aagagagaag gcguucugca ggccgcggau uucgacgaga augguuugau aaagucacuc      540

ccaaacacuc ccuggcaacu gagggcagcc gcucuggauc ggaaacucac gccuuuagag      600

uggagugccg ugcugcuuca cuugaucaag caucgugggu aucugagcca gcgcaaaaau      660

gaaggagaga ccgcagacaa ggaacuaggc gcucuccuga aaggcgucgc cgauaacgcg      720

cacgcccuuc agacagggga cuuuagaacu ccagcagagc uggcuuugaa uaaguucgag      780

aaggaauccg gacauauuag gaaccagcgg ggugauuacu cucacaccuu uucgcgaaaa      840

gaccuccaag ccgagcugau cuuacuguuc gaaaagcaga aagaguuugg caauccccac      900

gugucagggg gacucaagga aggcauugag acacuucuga ugacccagcg cccugcuuug      960

agcggugaug ccguacagaa aaugcuaggg cauuguacuu ucgagccagc agaaccgaag     1020

gccgcuaaaa acacauauac cgcggagaga uuuaucuggc ugacgaagcu caauaaccug     1080

aggauacuug aacaaggaag ugagcggccc cugacugaca ccgaacgcgc aacauugaug     1140

gacgagccuu acagaaaguc caaacucacu uaugcccagg cuaggaagcu guuaggccug     1200

gaggauaccg ccuucuuuaa aggccuacgu uacgggaagg acaaugcaga agccucuaca     1260

cucauggaga ugaaggcuua ucacgccauu agccgggcac uggaaaagga gggacuuaag     1320

gauaaaaagu ccccauugaa ccugucaccc gaacuccagg acgagaucgg uaccgcguuc     1380

agucuguuua aaacggauga ggacauuacu ggccgacuua aggauagaau ccagccugaa     1440

auccuggagg cuuugcucaa acauauuagc uucgacaagu ucguucaaau aucucugaag     1500

gccuuaaggc gcaucgugcc acugauggaa caggggaaac gguacgauga ggcaugcgcc     1560

gagauuuacg gagaccacua uggcaagaaa aauacagaag agaagaucua ccuacccccg     1620

auaccugcug augaaauuag aaaccccguc gugcucaggg cccuguccca ggcacgcaaa     1680

gugaucaacg gugucguucg acgguauggg ucaccagcuc guauccacau ugagaccgcc     1740

agagaagugg gcaagagcuu uaaggacagg aaagagaucg agaagcgcca agaagagaau     1800

cggaaagaca gagaaaaggc ggccgcuaaa uucagggagu acuuuccuaa cuucguagga     1860

gaacccaaga guaaagauau ucuuaaguug cgacuguaug agcagcagca uggcaagugu     1920

cucuacucug ggaaagagau aaaucuggga cgcuuaaacg aaaaggguua uguggagauc     1980

gaccacgcac ugccauuuuc ccggacuugg gaugacagcu ucaauaacaa aguccuugug     2040

uugggcucgg aaaaucagaa caaggggaau caaacacccu acgaguacuu uaacggaaaa     2100

gauaauucua gagaauggca ggaguucaag gccaggguug agaccucccg uuuuccucgc     2160

ucaaagaaac agcggauucu ccugcagaag uucgacgaag auggcuuuaa agagagaaac     2220

cuaaaugaca cgagguaugu gaaccgauuc cugugccaau uugucgcuga ucggaugcgc     2280

cucaccggua aggggaaaaa gagaguguuc gccaguaaug gccagaucac aaaccugcuu     2340

aggggauucu ggggcuugcg gaagguacga gcagaaaacg acagacauca cgcgcuggau     2400

gccguugugg ucgcuuguag cacuguggcc augcagcaga aaaucacccg cuuugucagg     2460

uacaaggaga ugaaugcauu cgacgggaaa acaauugaua aggagacugg agaaguguua     2520

caucagaaaa cccacuuucc acagccgugg gaguucuuug cucaggaagu uaugauacgu     2580

guguucggua agcccgacgg caagccugag uuugaagagg ccgacacacc agagaaacuc     2640

cggacccugc uugcagaaaa gcuguccucu agacccgagg ccguacacga auaugucacu     2700

ccuuuguucg ugagcagggc uccaaaccgc aaaaugucag ggcagggaca uauggagacg     2760

guuaagagug cgaaacgacu cgaugaaggc guguccgucc ugagagugcc ccuaacacaa     2820

cugaagcuca aagaccugga gaagauggua aaucgggaga gggaaccgaa acuuuacgag     2880

gccuugaaag cacgccugga agcucacaag gaugacccug ccaaagccuu ugcugagcca     2940

uucuauaagu acgauaaagc agguaaccgu acccaacagg ugaaggccgu uagagucgaa     3000

caagugcaga agacuggggu guggguccgg aaucauaacg gcaucgcgga caaugcuacc     3060

augguuaggg uggauguguu ugagaaagga gacaaguauu acuuaguacc cauuuacagc     3120

uggcaggucg ccaaaggcau ccucccugau cgcgcagugg uucaggggaa ggacgaggaa     3180

gauuggcaac ugauugacga uucuuucaac uuuaaauucu cacuucaccc caaugaccug     3240

guggagguca ucacaaagaa ggcccgaaug uucggauauu uugcuaguug ccacagaggc     3300

accgguaaca uaaauauccg gauucaugac cuagaucaca aaaucgggaa gaacggcauu     3360

cucgaaggaa ucggugugaa aacugcacug agcuuccaga aguaccagau agacgaguug     3420

ggcaaagaga uuaggccaug ucgccugaag aagagaccuc ccguuaggaa acggccagcc     3480

gcgacaaaga aagcugggca ggccaagaaa aagaaguaag gacuagugaa gggaacaccc     3540

aaauuuaauu cagccuuaag cacaauuaau uaagagugaa acguaauugu acaagcaguu     3600

ggucacccac cauagggcau gaucaacacc gcaaccuuuc cuuuuucccc cagugauucu     3660

gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa auuuaguaag cuuaaggcgg     3720

ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc ucucacuuuc aguaaauaaa     3780

auaaaagcag caacagaaau aaagaaauaa augaaauuca aaaugaaaua aauauugugu     3840

ugugcagcau uaaaaaauca auaaaaauua aaaaugagca agaucuaaaa aaaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu          3955


<210>  3517
<211>  3961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3517
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggca      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagua ccacguugga      240

auugacgugg guaccuucag cguaggccug gccgcgauag agguggauga cgcugggaug      300

ccaaucaaaa cacucucccu ugucucucac auucaugauu caggacugga cccggaugaa      360

aucaagagug ccgugacuag guuggcaagc uccggcauug cccggcgcac gcgacguuua      420

uauagaagga agcggcgcag acugcagcaa cuagacaaau uuauccagag gcaggguugg      480

cccguuauag agcucgaaga uuacucugac ccucuguauc cauggaaggu gcgggcugag      540

cuugcagccu cguacaucgc ugaugaaaaa gagcgcgggg agaagcuguc agucgccuug      600

cgacacauug cgagacauag gggauggcgg aaccccuaug caaaagugag cagucucuac      660

cugccugacg gcccauccga ugccuucaag gcuauccgug aagagauuaa gcgcgccucu      720

ggccagcccg uaccugaaac cgcaacaguu gggcaaaugg ugacucugug cgagcucgga      780

accuuaaaac ugagagguga gggcgggguc cuuagcgcua gguugcagca guccgacuau      840

gcccgggaaa uccaggagau augucgaaug caagaaauug gacaggagcu guaccgcaag      900

aucauugaug ugguguuugc ggccgaauca ccaaaaggca gugcaagcuc uagagucggu      960

aaggacccgc uacagcccgg gaaaaauagg gcucucaagg ccuccgacgc uuuccagcgg     1020

uaccgcaucg ccgcacugau cggaaaccuu agaguuaggg uggauggcga gaaacguauu     1080

cugucaguag aggaaaagaa uuugguguuu gaccaccucg ucaaccugac accuaagaaa     1140

gagccagaau gggugaccau agccgagauc uuaggcauug aucgggggca acugaucgga     1200

acugcuacaa ugaccgacga uggugaacga gcgggcgcaa gacccccuac gcacgacacu     1260

aauaggagca uaguuaacag ucgcauugcc ccacucgugg auugguggaa gaccgcuucu     1320

gcccuugagc agcaugcaau ggucaaagcc cuguccaaug cugaggugga cgauuucgac     1380

agccccgaag gggccaaggu acaggcauuu uucgcggauu uggacgacga uguucacgcu     1440

aaacuagacu cgcugcaucu cccgguggga cgggccgcau auucugagga uacacugguc     1500

agacuuacua ggcgcaugcu guccgacggc guggauuugu acaccgcccg acuccaagaa     1560

uuugguaucg agccuucaug gacaccccca accccucgga ucggggaacc cgucggcaac     1620

ccagcugugg accguguucu gaagacggug aguagauggu uagagagcgc cacuaagaca     1680

uggggagcac ccgagagggu aauuaucgaa cacguccgcg agggcuucgu gaccgaaaaa     1740

cgggcuagag agauggaugg ggacaugagg cgacgcgccg cgcggaaugc caagcuguuu     1800

caggagaugc aggaaaaacu aaacguucag ggaaagccuu ccagagcuga ucucuggagg     1860

uaucaaucug ugcagcguca gaauugccag ugugcauacu gcgguagccc aauuacuuuc     1920

ucaaacagug agauggacca cauagucccg cgcgccggcc aaggguccac aaauacccgg     1980

gaaaaccugg uggcuguaug ucauagaugc aaucagagca aaggaaacac gcccuuugcc     2040

aucugggcaa agaacaccuc uauugagggc gugucaguua aggaagcggu cgagaggaca     2100

cgacacuggg ugacugauac cgguaugcgg aguacagacu ucaaaaaguu cacuaaagcc     2160

guggucgagc gcuuucagag agcuaccaug gaugaagaga ucgacgccag gagcauggaa     2220

uccguugcau ggauggcuaa ugagcuucgg ucucgagugg cccagcauuu cgcaucgcac     2280

gggacaaccg ugagaguaua ucgcggcagc uugacugccg aagcuaggcg ugcguccgga     2340

aucucaggca agcugaaauu uuucgacggg gucggaaaga gucggcucga uagaaggcau     2400

cacgccauug acgcagcugu gauagccuuu acgucugauu acguugccga gacacuggcu     2460

gugcgcagca accuuaagca aucccaggca caccgacagg aggccccuca guggagagag     2520

uucaccggua aagacgcgga acaucgggcu gccuggaggg ucugguguca aaagauggaa     2580

aaacugucag cauugcucac ugaggaucug cgcgacgauc gugugguugu gaugagcaau     2640

guaagauuac ggcugggcaa cgggucugcc cacaaggaaa ccaucggaaa acuauccaag     2700

gucaaacuca guagccagcu gucugugucc gacauugaua aggcuucaag ugaggcacuu     2760

uggugcgccu ugacaaggga gccaggcuuu gaccccaagg aaggucugcc ugcgaaucca     2820

gagcgccaua uccgagugaa cgggacccac gucuaugcug gcgauaauau uggacucuuc     2880

cccguuagcg ccggcucgau cgcacugaga gggggauacg ccgaauuagg cuccucuuuu     2940

caccaugcuc ggguguacaa aauaacuagc gguaagaaac cggccuucgc aaugcugagg     3000

guauauacaa ucgaccuuuu gccuuaccgc aaccaggauc ucuuuucagu ggagcugaag     3060

ccacaaacga ugaguaugag acaggcugag aaaaagcuaa gggacgcccu ggcgaccggg     3120

aaugccgaau aucucggaug gcuggucgug gacgaugagc uuguugugga cacuuccaag     3180

auugcaacag aucaggucaa agcuguggaa gccgaguugg gcaccauccg gcguuggcgc     3240

guugacgguu ucuuuucucc cagcaagcug cgauuaagac cucuccagau guccaaagaa     3300

ggcauuaaga aagagucagc ucccgagcug agcaagauca uagauaggcc aggguggcuu     3360

ccugcaguga acaagcuguu cagugacgga aaugucacag uagugcggcg cgauucuuug     3420

ggcagaguua ggcucgaauc cacugcccau cugcccguga ccuggaaagu ccaaaagcgg     3480

ccagccgcua cgaaaaaggc ggggcaggcc aaaaagaaga aauaaggacu agugaaggga     3540

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa     3600

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu     3660

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua     3720

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua     3780

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     3840

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau     3960

u                                                                     3961


<210>  3518
<211>  3661
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3518
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagccag gauucuggcg      240

uucgacauag gaaucagcuc cauugguugg gcuuuuucug agaacgauga acucaaggac      300

ugcggcguuc ggaucuucac caaaguggag aauccaaaga caggggaauc acuugcccug      360

ccgcgccgau uggcacguag ugccagaaag agguuagcuc ggcgcaaagc aagacugaac      420

caucuaaagc accucauugc caaugaguuu aaacugaacu acgaagauua ucagagcuuc      480

gacgaguccc uugcuaaggc cuacaaagga ucucugaucu cgcccuauga guugagguuu      540

cgggcgcuca augaacugcu gucaaagcaa gauuucgcac gcguaauacu ccauaucgcc      600

aagcgaagag gcuacgacga uauuaaaaac agcgacgaua aggagaaagg ugcuaucuua      660

aaggccauua aacagaauga agagaagcug gcaaacuauc agaguguggg ggaguaccuu      720

uauaaagaau acuuucagaa guucaaggaa aauuccaaag aauuuacuaa cgucaggaau      780

aagaaagagu cuuacgaacg guguaucgcu cagagcuucu ugaaggacga gcugaaacua      840

auauuuaaga agcaacguga guucggauuu uccuucucua aaaaguucga agaggaagug      900

cucaguguug ccuuuuauaa acgcgcgcug aaggauuuca gccaccuugu gggcaacugc      960

ucauuuuuca cggacgagaa aagagccccu aaaaauuccc cacuggcauu uauguucguc     1020

gcuuugacca ggauuaucaa ccuccugaau aacuuaaaga acacagaagg cauucuguac     1080

acuaaagaug accucaaugc ccuucugaac gagguguuga agaaugggac ccuaacauau     1140

aaacagacca agaaacugcu cggacuguca gacgauuacg aguuuaaggg ugaaaagggc     1200

acuuauuuca ucgaguuuaa aaaguacaaa gaguucauca aggcucuugg ggagcauaac     1260

cugagccagg acgauuugaa ugaaauugcc aaagacauaa cacucaucaa ggaugagauu     1320

aaacugaaga aggcauuagc caaauaugac cugaaccaaa aucagaucga uagucuaucu     1380

aagcucgagu uuaaagacca ccugaacaua uccuucaagg cucuuaaauu gguaacuccc     1440

cugaugcucg aaggaaagaa guacgaugag gcguguaaug aacugaaccu uaaaguugca     1500

auuaaugagg acaagaaaga uuuucugccu gccuucaacg agacguacua uaaggacgaa     1560

gugacuaauc cagucguguu gcgggcuauc aaagaguacc gaaaggugcu caacgcccug     1620

uuaaagaaau auggcaaggu ccacaaaauc aauauugaac uggcacgcga gguugguaag     1680

aaccauagcc agagagccaa aaucgaaaag gagcagaacg agaauuacaa ggcuaaaaag     1740

gacgccgaac uagagugcga aaaacucggg cugaagauua acucgaaaaa uauacuuaag     1800

uugaggcugu ucaaagagca aaaggaauuu ugugcauauu cuggagagaa gaucaaaauu     1860

uccgaucucc aggacgagaa gaugcuggaa aucgaucaca ucuaccccua uucacggagu     1920

uucgacgaua gcuacaugaa caaaguguua guauuuacca agcagaauca agagaaacug     1980

aaccagacac cuuucgaagc guuuggcaau gacuccgcua aguggcagaa gauugaggug     2040

cuugccaaaa acuugccaac uaagaaacag aagcgcauac ucgauaaaaa uuacaaggac     2100

aaggaacaaa agaacuucaa ggauagaaau cugaacgaca ccagguauau cgcacgucua     2160

guccugaauu acacaaaaga uuaucucgac uuucugccgc uuucugauga cgagaacacc     2220

aaguugaaug acacgcagaa aggcagcaag gugcauguug aggccaaguc agggaugcug     2280

accagugcuu uacggcacac auggggauuc uccgccaaag aucgaaacaa ccaccuccau     2340

cacgcaauug acgcugugau cauugccuac gcgaauaaca gcaucgucaa ggccuuuucu     2400

gauuucaaaa aggaacagga gucaaauagu gcugaacugu augccaaaaa gauaagcgag     2460

cuugacuaca agaacaaaag aaaguuuuuc gagcccuuuu ccgguuucag gcagaaagug     2520

cuggauaaga ucgacgaaau uuucguaucu aaaccugagc gcaagaaacc aucgggcgca     2580

uugcaugaag agaccuuucg gaaggaagag gaguucuauc aaagcuacgg gggaaaggaa     2640

ggcguucuca aagcucugga gcuagguaag aucagaaaag ugaaugggaa gauugucaaa     2700

aacggcgaua uguuuagggu ggacaucuuc aagcacaaga aaacuaauaa guuuuacgcc     2760

guccccauau auacaaugga uuucgcacug aaagugcucc cuaacaaggc gguugcccgc     2820

uccaaaaagg gagaaauuaa ggacuggauc cugauggaug agaauuacga auuuugcuuc     2880

ucacuuuaua aagacaguuu gauucugauc cagaccaagg auaugcagga gccagaguuu     2940

guguacuaua acgcuuucac gucuagcacc guauccuuaa uagucucaaa acaugacaau     3000

aaguuugaaa cacucagcaa aaaccagaag auccuguuca agaaugccaa cgagaaagaa     3060

gugauugcaa agucuaucgg cauucaaaau cuuaaaguuu ucgagaagua caucgugucc     3120

gcucuggggg aagucacuaa agccgaguuu cgacagcggg aggacuucaa gaaaaagcgu     3180

cccgcagcca ccaagaaagc uggacaggcg aagaaaaaga aauaaggacu agugaaggga     3240

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa     3300

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu     3360

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua     3420

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua     3480

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     3540

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau     3660

u                                                                     3661


<210>  3519
<211>  4072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3519
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaagcga ccugguucuc      240

ggacuugaua uugguauagg cuccgugggg guaggaaucc ugaacaaggu gaccggcgag      300

auuauccaua aaaauucuag gauuuuccca gccgcgcagg cugaaaacaa uuugguccgg      360

cgcacaaacc gacaaggucg uagauuagcc aggcggaaga agcaccgcag agugaggcug      420

aaucggcuau uugaggaauc agggcucauc acugacuuca cgaaaauaag uaucaaccug      480

aauccguacc agcuucgcgu uaagggacug accgaugagu ugagcaacga agaacucuuu      540

auugcacuga aaaauauggu gaagcaucga ggcaucuccu aucuggacga ugccucugac      600

gauggcaacu cgucagucgg ggacuacgcu cagauuguga aagagaauag caagcagcuc      660

gaaacaaaga cucccggaca aauccaguua gagagauauc agaccuacgg ucagcugagg      720

ggcgauuuca caguagaaaa agacgggaag aaacaccggc uuauaaacgu uuuuccuacc      780

agugcauauc guuccgaggc cuugcgcauu cugcaaacuc agcaggaguu caauccacag      840

aucacagaug aauuuauuaa cagauaccua gagauccuca ccggaaagag gaaauauuac      900

cauggccccg guaaugaaaa gucucggacg gacuacgggc gauaucgcac uagcggagag      960

acccuggaua acaucuucgg cauucuuaua ggcaaaugca cauuuuaccc ugacgaguuc     1020

agagcugcca aggcguccua uacugcacaa gaguuuaacc uguugaauga ccucaacaau     1080

cugaccgugc caacagagac caagaaauua ucaaaggaac agaaaaacca gaucauuaau     1140

uacgucaaga acgagaaagc cauggggccc gcuaagcugu ucaaguauau cgccaaacuc     1200

cuuaguugug auguggcaga cauaaaggga uacaggauug auaaaagcgg uaaggcugaa     1260

auccacacgu uugaggccua ucggaaaaug aagacucugg aaacauugga caucgagcaa     1320

auggaucgcg agacccuaga caagcuggcg uacgugcuca cucugaauac agaaagagag     1380

ggcauucagg aagcccuuga gcacgaguuc gcagaugggu cuuucuccca gaaacagguc     1440

gacgagcugg uucaauuuag gaaggcuaac ucaagcaucu ucggaaaagg cuggcauaau     1500

uuuaguguga aguugaugau ggagcucauu ccugaacugu acgagaccuc ugaagaacag     1560

augacgauau uaacccgucu ggguaaacag aagacaacuu ccagcucgaa caagaccaaa     1620

uauaucgaug agaagcuacu cacagaagag auuuacaauc caguaguggc caaaucuguc     1680

cggcaggcua ucaagaucgu gaacgccgca auuaaagaau auggggacuu cgauaauaua     1740

guuaucgaga uggcccgaga aacuaacgag gacgacgaga agaaagcuau ucaaaagauc     1800

cagaaggcga auaaagauga aaaggacgca gccaugcuga aagcugccaa ccaguacaac     1860

ggcaaggcag agcuuccgca cuccguguuu cauggacaca aacaguuggc caccaagauu     1920

agacuguggc accaacaggg cgaaaggugc cucuauacag ggaagaccau cucaauacau     1980

gaucugauca auaacaguaa ucaguucgag gucgaccaca uucuuccccu gagcaucacu     2040

uuugaugacu ccuuggcuaa caaagugcuc guauacgcca cggcaaauca ggaaaaggga     2100

caacgcacac cuuaucaggc gcuggauucu auggacgaug cuuggagcuu ccgggaguua     2160

aaagccuuug uuagagaguc aaagacccug aguaacaaaa agaaggaaua ccuacucacu     2220

gaggaagaca uuuccaaauu cgaugugagg aagaaauuua ucgagcgcaa ucuggucgac     2280

acccgauacg caagccgggu gguccuuaac gccuugcagg aacauuuccg ugcucacaag     2340

auagauacaa aagugucugu ugugagaggu caauuuaccu cacagcugag gcgccauugg     2400

ggcauugaga agacucggga cacauaucac caccaugccg uagacgcacu caucauugcu     2460

gccaguagcc agcugaauuu auggaagaaa cagaagaaca cgcuggucuc cuacucugag     2520

gaucaacuuu uggacaucga aaccggggag cucauaucgg augacgaaua uaaagagagc     2580

guguucaagg cgccauacca gcacuucguu gauacucuga aauccaagga guuugaagac     2640

ucaauccuau ucaguuauca gguggauucu aaauuuaaua gaaagauuag cgacgccaca     2700

aucuacgcua ccaggcaggc aaaggucgga aaagauaagg ccgacgagac auaugugcug     2760

ggcaaaauua aggauaucua cacucaagac gguuacgaug cuuucaugaa aauauauaag     2820

aaggacaaau ccaaguuucu cauguaccga caugaccccc agaccuucga aaaaguaauu     2880

gagccuaucc uggaaaacua uccaaauaag cagaucaacg agaaagggaa ggaggugccc     2940

uguaauccgu uucuuaagua caaagaggaa cacggcuaua uucgcaagua cagcaaaaag     3000

ggaaacggcc cugaaaucaa aucauugaag uauuacgauu cuaagcuggg gaaccacauu     3060

gacauaacgc ccaaagauuc caauaacaag guugucuuac agagugugag cccauggcgg     3120

gccgacgugu acuucaauaa aaccacagga aaguaugaga uccucggucu gaaauacgca     3180

gaucuucaau uugaaaaggg cacugggacc uauaagauuu cucaggagaa auacaacgac     3240

aucaagaaaa aggagggagu cgauuccgac ucagaguuca aauuuacacu guauaagaau     3300

gauuugcucc ugguuaaaga cacugaaacc aaggaacagc aacuauucag auuucugagu     3360

aggacgaugc cuaagcagaa acauuacgug gagcucaagc ccuaugauaa acagaaguuc     3420

gaaggcggug aggcgcugau uaaagugcuu gggaacguag ccaauagcgg ccagugcaag     3480

aagggauugg gcaaaucgaa caucuccaua uacaaggucc guacagacgu gcuggggaau     3540

caacacauca uuaaaaacga gggagacaag ccaaaauuag auuucaagcg ccccgcugcc     3600

accaagaaag caggccaggc uaagaaaaag aaauaaggac uagugaaggg aacacccaaa     3660

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu     3720

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa     3780

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu     3840

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     3900

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     3960

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     4020

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             4072


<210>  3520
<211>  4630
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3520
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaccca guucgaggga      240

uuuacaaacc uguaccaagu uagcaagacu cucagguucg aacuuauucc acaggguaaa      300

acgcugaagc auauacagga gcagggcuuu aucgaagagg acaaggcccg gaaugaucac      360

uauaaagaau ugaagccgau uaucgaccgc auuuacaaaa ccuaugcgga ucaaugcuua      420

cagcuggugc agcuagacug ggagaaccuc uccgcugcca ucgauucuua ccgaaaggag      480

aaaacagaag agacucguaa ugcacugaua gaggaacagg ccaccuauag aaacgcuauc      540

caugacuacu ucauugggag gacagauaau cuuaccgacg caaucaacaa gcggcacgcc      600

gagauuuaua agggacuguu uaaagcugaa uuguucaaug gcaagguacu caaacaacug      660

gguacuguga caaccacgga gcaugaaaac gcccugcucc gcucauuuga uaaguucacu      720

accuacuuua guggguucua cgagaauagg aaaaacgucu uuagcgcgga agacaucucc      780

acagcaauac cccacagaau ugugcaggau aauuucccua aguuuaaaga gaacugucac      840

aucuucacuc gguuaauuac cgccguucca ucucugcgcg agcauuucga aaaugugaag      900

aaggcuaucg gaaucuuugu cucgacauca auugaggaag uguucagcuu ucccuucuau      960

aaccagcuuu ugacccagac gcaaauagac cuguacaauc agcuacucgg cggcaucagu     1020

cgagaggccg ggacugagaa aauuaaggga cugaacgaag uacuuaaccu ggcaauccag     1080

aaaaaugaug agacagcuca cauaauugcc uccuugccuc auagauuuau cccacucuuc     1140

aagcaaaucc ugucugacag gaacacccuu agcuuuauuc uggaagaguu caaauccgac     1200

gaagagguua uccagucauu uugcaaguau aagacucucc uucggaauga aaacgugcug     1260

gagacagcgg aggccuuguu caaugaacua aacaguauug aucugaccca cauauuuauc     1320

agccacaaaa agcucgagac gauuucuucc gcacugugug accauuggga uacccuucgu     1380

aaugcucugu acgaacgcag aaucucagag uugacaggua aaaucacuaa gagcgccaaa     1440

gagaaggucc agaggagucu caagcacgaa gacauuaacc ugcaggagau aaucucugcu     1500

gccggcaaag aauuauccga ggcauucaag cagaaaacca gcgaaauucu gucgcaugcc     1560

cacgcugcgc uagaucaacc ccucccuaca acucugaaga aacaggagga gaaggaaauc     1620

cuuaagucuc aguuggacuc ccugcucggg cuguaucauc uucuggauug guuugcagug     1680

gacgagucaa augaagugga uccagaguuc agugcccggu ugaccggaau uaaacucgaa     1740

auggagccga gccuguccuu cuacaacaag gcucgaaauu augccacuaa aaagcccuac     1800

ucugucgaga aauuuaaguu aaacuuccaa augccuaccc uggcaagcgg cugggacguu     1860

aauaaagaaa agaacaaugg ugccauccua uuugugaaga acgggcucua cuaucuggga     1920

auaaugccaa aacagaaggg ccgcuacaaa gcucuuucau ucgagcccac ugaaaagacg     1980

agugagggcu uugauaaaau guauuacgac uauuucccug acgccgcaaa gaugauccca     2040

aagugcucca cacaguugaa agcgguaacc gcucacuuuc agacucacac cacacccauu     2100

cugcucagca acaauuucau cgaaccgcug gagauuacca aggagaucua cgauuuaaac     2160

aauccugaaa aagagcccaa gaaauuucaa acugccuaug caaagaagac aggggaccag     2220

aaaggauaca gagaagcccu guguaagugg auagauuuca cgagggacuu ucuuucuaaa     2280

uacaccaaga cuacaucaau ugauuugagu agccuccggc cauccucuca guauaaagac     2340

cugggugagu acuaugcuga gcuaaacccu cugcucuacc auaucucguu ccagcgcauu     2400

gccgaaaagg agaucaugga ugcaguggaa accggcaagc uguaucuuuu ucaaauauac     2460

aauaaagacu ucgcuaaggg gcaccaugga aaacccaacu ugcacacacu guauuggacu     2520

ggcuuauuca gcccagagaa ucucgccaag accuccauca aacugaacgg ucaggcggaa     2580

uuauuuuaca gacccaaguc aaggaugaaa cguauggccc accggcuggg ggagaagaug     2640

uugaauaaga aacucaagga ucagaaaacg ccuauuccag acacccugua ccaagagcua     2700

uaugauuacg ucaaccaucg acugagucac gaccucucug augaagcuag agcacugcuu     2760

ccgaauguga ucacaaagga gguuagccau gaaauuauca aagacaggcg cuucacuucc     2820

gacaaguuuu ucuuucacgu gcccauaacc uugaacuauc aggccgcuaa uucaccuagc     2880

aaguucaacc agcgggucaa ugccuaccug aaagagcauc cagaaacacc cauuaucggc     2940

aucgauagag gagagaggaa cuuaauuuau aucacuguga uugacucuac cggcaagaua     3000

cucgagcagc gcucccugaa cacgauccaa caguuugauu accagaaaaa gcuggacaau     3060

cgagaaaaag agcggguagc agcgcgucag gccuggagug uuguggggac aauuaaggau     3120

cuuaagcaag gauaucuaag ccaggucauc cacgaaauug uggaccucau gauccacuac     3180

caggcugucg ugguucugga gaacuugaau uucgguuuua aaucuaagag aaccggcaua     3240

gccgaaaaag caguguacca gcaauucgag aagaugcuga ucgauaaauu aaacugccuc     3300

guacugaagg acuauccugc ugagaagguc gggggagugc uuaauccaua ccagcugacu     3360

gaucaguuua cauccuucgc caaaaugggc acccaaucag guuucuuguu uuauguuccc     3420

gcaccguaca caaguaagau ugacccucuc accggguucg uggauccauu ugucuggaaa     3480

acaaucaaga accaugaaag caggaaacac uuccuggagg gcuuugacuu ccuacauuau     3540

gaugugaaga cgggagacuu uauucugcac uucaagauga aucgcaaccu uucguuucag     3600

cggggccucc ccggguucau gccugccugg gacaucguau uugagaaaaa ugaaacccag     3660

uucgaugcua agggaacucc cuuuauagcg gguaaaagaa uugugccagu uaucgagaac     3720

cauagguuca caggccgaua ccgcgaccug uauccugcca augaauugau cgcacuguua     3780

gaggaaaagg ggauugucuu ccgggaugga uccaacauac uccccaaacu gcuugagaau     3840

gacgauucuc acgcuaucga caccauggug gcccugauua gaagcguguu gcagaugagg     3900

aacucaaaug ccgcuacagg cgaagauuac aucaacaguc caguccguga ccucaacggu     3960

guuuguuuug auucccgcuu ccaaaauccg gaguggccua uggacgcaga ugccaacggg     4020

gcguaccaca uugcucugaa gggccagcua cugcuuaauc aucucaaaga gucuaaggac     4080

cugaaguugc agaacggaau cagcaaucag gacugguuag ccuauauaca agaacugcgg     4140

aacaaaagac ccgcagccac uaagaaagcu ggccaggcaa agaaaaagaa guaaggacua     4200

gugaagggaa cacccaaauu uaauucagcc uuaagcacaa uuaauuaaga gugaaacgua     4260

auuguacaag caguugguca cccaccauag ggcaugauca acaccgcaac cuuuccuuuu     4320

ucccccagug auucugaaaa accccucuuc ccuucagcuu gcuuagaugu uccaaauuua     4380

guaagcuuaa ggcggccuac agaagaaaaa gaaaaaaaag gccacaaaag uucccucuca     4440

cuuucaguaa auaaaauaaa agcagcaaca gaaauaaaga aauaaaugaa auucaaaaug     4500

aaauaaauau uguguugugc agcauuaaaa aaucaauaaa aauuaaaaau gagcaagauc     4560

uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaagaauu                                                            4630


<210>  3521
<211>  4609
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3521
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaagcau uuaccaggag      240

uucguuaaca aguauucccu gucuaaaacc cucagguuug aacuuauacc acaaggaaag      300

acacuggaga auaucaaggc ccgggguuug auuuuagacg augaaaaacg cgcgaaggac      360

uacaaaaagg cuaaacagau cauugauaag uaucaucagu ucuuuaucga ggaaauacug      420

ucaagugugu gcaucagcga ggaccuacuc cagaacuacu ccgauguaua uuucaagcug      480

aaaaagucug acgaugacaa ucuucaaaaa gauuuuaagu cggccaaaga cacuauuaag      540

aaacagaucu cagaguacau uaaggauagc gaaaaguuca aaaaccuguu uaaucagaac      600

uugaucgacg caaagaaagg ccaggagagu gaucucauac uguggcugaa gcaauccaaa      660

gacaauggga uugaacucuu caaggccaac ucugacauca cggauauuga cgaggcuuua      720

gagaucauca agagcuuuaa aggauggacc acauauuuca agggcuuuca cgaaaaucgu      780

aaaaacgugu acuccucaaa ugauauuccg acaaguauaa ucuaccgaau ugucgacgau      840

aaccugccca aguuccuuga gaauaaagca aaguaugaga gcuugaagga caaagccccu      900

gaggcuauca acuacgaaca gauaaagaaa gaucuggccg aggagcuaac cuuugacauu      960

gauuauaaga caucugaagu gaaucagaga guuuucuccc ucgacgaggu guucgaaauc     1020

gcgaacuuua acaauuaccu gaaccaguca gguaucacca aauucaauac uauuaucggg     1080

ggaaaguuug ucaacggcga gaauacaaag aggaaaggca uuaacgaaua uauaaaucuu     1140

uacagccaac agaucaacga uaagacccug aaaaaguaua aaaugagugu guuguucaag     1200

cagauucucu cugacacgga guccaagagc uuuguaaucg acaaacugga ggaugacucg     1260

gauguuguga cuaccaugca aucuuucuac gaacagaucg cagccuuuaa gacagucgag     1320

gaaaaaucca uuaaggagac uuuaucacuc cuguucgacg aucuuaaagc ucagaagcug     1380

gacuugagua aaauauacuu uaagaaugau aagagccuaa ccgaccuguc ccagcaagug     1440

uucgaugacu auucugugau cgggacagcc guccucgaau acauuaccca gcagaucgca     1500

cccaaaaacc uggauaaucc aagcaagaaa gagcaggagc uuauugcuaa gaaaacggaa     1560

aaggccaagu aucugucauu ggagacuauc aaacucgcgc uggaagaguu uaacaagcau     1620

cgggacauag auaaacaaug ucgcuucgag gaaaucuuag ccaauuuugc agcuauuccu     1680

augaucuucg acgagauugc ccagaacaag gacaaucugg cucagaucag uauaaaauac     1740

cagaaccaag gaaagaagga ucuacuccag gccuccgcag aagacgaugu uaaagccauu     1800

aaggaccugc uugaucagac aaacaauuug cugcacaaac ucaagaucuu ccauauuagc     1860

cagucugagg acaaagcuaa cauccuggau aaggacgaac acuuuuaucu uguguucgag     1920

gagugcuacu uugaacuggc gaauauagua ccauuguaua acaaaaucag aaauuacauu     1980

acccaaaagc ccuacucaga ugagaaguuc aaacucaacu uugaaaauag uacucuggca     2040

aacgguuggg acaagaauaa agagccugau aacacagcca ucuuauucau uaaggacgau     2100

aaauauuacc ugggcgugau gaauaagaag aacaauaaaa ucuuugacga caaggcuaua     2160

aaagaaaaca agggggaggg auauaaaaag auugucuaca agcuacuccc aggcgccaau     2220

aaaaugcugc cgaagguguu cuuuagcgca aaauccauca aguucuauaa ccccucugag     2280

gauauccuua ggauucggaa ccacucgacc cauacgaaaa augguagccc ucagaagggg     2340

uacgaaaagu uugaguucaa caucgaagac ugucgcaaau uuauugauuu cuauaagcag     2400

uccauaucaa aacacccaga guggaaggac uucggauuuc gauucaguga uacccaaaga     2460

uacaauucua ucgacgaauu uuacagggag guugagaacc agggcuauaa auugacauuc     2520

gaaaauauua gcgaguccua caucgauuca guggucaacc agggcaagcu guaucucuuu     2580

cagauuuaca auaaggacuu cagcgccuau ucuaaagggc ggcccaaccu gcauacuuua     2640

uacuggaagg cucuguuuga ugaacguaau cuucaagacg ugguauauaa auugaacgga     2700

gaggccgaac ucuucuaccg caagcagucc aucccuaaaa agauaaccca cccagcaaaa     2760

gaagcgaucg cuaauaagaa caaggauaau cccaaaaagg agaguguuuu ugaauacgac     2820

cugauuaaag acaagagauu cacagaggau aaauuuuucu uccacugccc gaucacuauu     2880

aacuuuaaga gcucuggugc caauaaguuc aacgacgaaa ucaaccuacu gcucaaagag     2940

aaggcaaaug augugcauau acuguccauu gacaggggcg agcggcaccu ugccuauuac     3000

accuuggucg augggaaagg aaacaucauc aagcaggaca cauuuaauau uauaggcaac     3060

gaucgaauga aaaccaauua ucaugacaag cuggcugcca ucgaaaagga ucgcgacuca     3120

gcaagaaaag auuggaagaa aauuaacaau aucaaggaga ugaaagaagg uuacuuaagu     3180

cagguggucc acgagauugc uaagcucgug aucgaauaua acgccauagu uguguucgag     3240

gaccugaauu uuggguucaa gaggggccgg uuuaaaguag agaagcaagu cuaccagaaa     3300

cuugaaaaga ugcugaucga gaaauugaac uaucucgugu ucaaggauaa ugaauuugac     3360

aaaacuggag gcguucugcg cgcguaccag cuaacggccc cuuucgagac auuuaagaag     3420

auggggaaac agaccggaau uaucuacuau gugcccgcug guuucacuag caagauuugu     3480

ccagucaccg gcuuugugaa ccaacuguac ccuaaauaug aaucgguauc caagucucag     3540

gaguucuuca gcaaauuuga caagaucugc uacaaucucg auaaggggua uuucgaguuu     3600

ucauucgacu acaaaaacuu uggagauaag gcagccaaag gcaaguggac aauagcuagu     3660

uucgguucca gacugauuaa uuuuaggaac ucugacaaaa aucauaacug ggauacccgu     3720

gaaguguauc ccacuaagga gcuugaaaag uugcugaaag acuacagcau cgaguacggg     3780

cacggcgagu guaucaaggc cgcaauuugc ggagaauccg auaaaaaguu cuuugcgaaa     3840

uuaacaucag uucucaacac gauccugcag augcggaaua gcaagaccgg cacugagcuu     3900

gacuaucuga uaaguccagu cgccgaugug aacgggaauu ucuuugacuc ucgacaagcu     3960

cccaagaaca ugccucagga ugccgacgca aauggagcuu accacauugg ucuaaaaggc     4020

cucaugcugu uggggagaau caagaacaau caggaaggca aaaagcugaa cuuagugauu     4080

aaaaaugagg aauauuucga guuuguccag aacaggaaua acaagcgccc agccgcaacu     4140

aaaaaggccg gacaagcuaa gaaaaagaaa uaaggacuag ugaagggaac acccaaauuu     4200

aauucagccu uaagcacaau uaauuaagag ugaaacguaa uuguacaagc aguuggucac     4260

ccaccauagg gcaugaucaa caccgcaacc uuuccuuuuu cccccaguga uucugaaaaa     4320

ccccucuucc cuucagcuug cuuagauguu ccaaauuuag uaagcuuaag gcggccuaca     4380

gaagaaaaag aaaaaaaagg ccacaaaagu ucccucucac uuucaguaaa uaaaauaaaa     4440

gcagcaacag aaauaaagaa auaaaugaaa uucaaaauga aauaaauauu guguugugca     4500

gcauuaaaaa aucaauaaaa auuaaaaaug agcaagaucu aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                 4609


<210>  3522
<211>  4497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3522
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc agacaagaaa uacagcauug gacuggauau agguacaaac      180

uccguuggcu gggccgugau cacagacgag uauaagguac caucuaagaa auucaaggug      240

cucgggaaua cugauaggca uucaauuaaa aagaaccuua ucggagcgcu guuguuugac      300

aguggcgaaa cggcugaggc cacccgguua aaacgcacag cacgacguag auacacuagg      360

cggaagaauc gcauuugcua ucugcaggaa aucuucagca acgagauggc caaggucgau      420

gacuccuuuu uccacagacu agaagagucu uuucucgugg aggaagauaa aaagcaugag      480

aggcacccga uauucgguaa uaucguugac gaaguggcuu accaugagaa auaucccacc      540

auuuaccauc ugcggaagaa acuugucgau ucgacagaca aggcagaucu gcgcuugauc      600

uaucucgccc uggcucacau gauuaaauuu cgagggcacu uccugaucga gggagaccuc      660

aacccugaua auucagacgu ggacaaguua uuuauacaac ugguacagac cuacaaccag      720

cuuuucgaag agaauccaau uaacgccagc ggcguugaug cgaaggcaau cuugagugcc      780

agacugucca aaucuaggcg gcuagaaaau cucauugcuc agcugcccgg cgagaagaaa      840

aacgggcuuu uuggaaaucu gaucgccuug agccucgguc ugacuccuaa cuucaagucc      900

aauuuugacu uagcagaaga ugcuaaacug caacucucaa aggacacaua cgaugacgau      960

cuugacaacc uguuggccca gaucggcgau caguaugcgg accuauuccu ggccgcaaag     1020

aaccucagug augcuauucu gcuuagcgac auacugcgug ugaauaccga gaucacgaaa     1080

gccccauugu cugcuuccau gauuaagcgc uacgacgagc accaucagga ucucacucug     1140

uuaaaagccc uggucagaca acagcuaccc gaaaaguaua aagagaucuu cuuugaccag     1200

ucaaagaacg gguacgcagg auauauagau ggcggugcca gccaggaaga guucuacaag     1260

uuuauuaaac cuauccucga aaagauggac gggaccgagg agcugcuugu gaaauugaau     1320

agggaagauc ugcuccggaa gcaacgaaca uucgacaacg gaaguauccc acaccagauu     1380

caccugggcg agcuucaugc uauccugcgc agacaggaag auuuuuaucc guucuugaaa     1440

gacaauaggg agaagauuga aaagauacuc acuuuucgga uccccuacua cgugggcccu     1500

cuggcgcgcg ggaacucuag auucgcaugg augaccagga aauccgagga gacaauuacc     1560

ccauggaauu uugaagaggu cguugauaag ggagccagcg cucaaucguu caucgaacgu     1620

augacgaacu uugacaaaaa uuuacccaac gagaaggugc ugccuaaaca cucucuacuc     1680

uaugaguacu ucacuguaua uaaugaacug acaaagguga aauacgucac cgaggguaug     1740

cggaagccag ccuuucuuuc cggcgaacag aagaaagcaa ucguggauuu gcuguucaag     1800

acuaaccgaa aaguuacagu gaagcagcuc aaagaggacu auuucaagaa gauugaaugu     1860

uuugauucag ucgagauaag ugggguggag gacagauuca augccagccu gggaaccuac     1920

caugacuuac ugaaaaucau uaaggauaaa gacuuucuug auaacgaaga gaaugaagac     1980

aucuuggagg auauuguacu cacgcugacc cuauucgaag acagggagau gaucgaggaa     2040

cgccugaaga cauaugcuca ccucuuugau gacaaaguua ugaagcagcu gaagcggaga     2100

agguacacug gcuggggucg ccuuucccga aaauugauaa acgggauccg ggauaagcaa     2160

ucuggcaaaa ccauucugga cuucuuaaag agcgauggau uugccaaccg uaauuucaug     2220

cagcucaucc augacgauuc acugacauuu aaagaggaca uucagaaggc acaggugagu     2280

ggccaagggg acucccuuca cgaacacauc gcgaaccugg cuggaagccc cgccauaaag     2340

aaagguauuu ugcagacugu caaggugguc gaugagcucg ugaaaguuau gggcagacau     2400

aagccggaaa auaucgugau ugagauggca agggagaacc agaccacaca gaaagggcaa     2460

aagaacucuc gcgaacggau gaaaagaauc gaggaaggaa uaaaggagcu gggcucacag     2520

auccuaaagg agcacccugu agaaaauacc cagcugcaga augagaaacu cuaccuguau     2580

uaccuucaaa acgguaggga cauguauguc gaucaggaau uggacauuaa ucgacugagu     2640

gauuacgacg uggaucauau cguuccccag agcuucuuaa aggacgauuc cauugacaac     2700

aaagugcuca cucgcucuga uaagaaucgg gggaaaucgg acaacguccc aagcgaggaa     2760

gugguaaaga agaugaaaaa uuauuggaga caacugcuua acgccaagcu gaucacgcag     2820

aggaaauuug acaaucuaac aaaggcugag cguggcggac ucuccgagcu ggauaaagcc     2880

ggcuucauaa agcgccaguu gguggaaacc cggcagauua cuaagcacgu ugcacaaauc     2940

cuggacucaa gaaugaacac caaauacgau gagaacgaca aguuaaucag ggaagucaaa     3000

gugauuacac ucaagaguaa acuggugucu gauuuucgaa aggacuucca guucuauaag     3060

guccgggaga ucaauaacua ccaccaugcu cacgaugccu accuuaaugc gguugugggg     3120

accgcccuga uuaaaaagua uccuaaauug gaaagcgagu uuguguacgg agacuauaag     3180

guauacgaug uccgcaaaau gauagcuaag uccgagcagg aaaucgguaa ggcaacugcc     3240

aaauauuucu uuuacucaaa cauuaugaau uucuuuaaga cagagaucac gcucgcuaac     3300

ggcgaaauua gaaaaaggcc ccugaucgag accaaugggg aaacuggaga gauagugugg     3360

gacaagggcc gggauuucgc cacaguucga aaagugcuaa gcaugccaca ggucaacauc     3420

gugaagaaaa ccgagguuca aacagguggg uuuucuaagg aauccauucu gcccaagaga     3480

aauagugaca aacuuaucgc acgcaagaaa gauugggacc cuaagaaaua uggcggauuc     3540

gacagcccaa cuguggcgua cucuguacuc gucguggcca agguggagaa gggcaaaucc     3600

aagaaacuga agucagucaa agaauugcug gggauuacca ucauggagag gaguagcuuu     3660

gagaagaacc cgauagauuu cuuagaagcu aagggauaca aagagguuaa gaaagaccuc     3720

auuaucaagc ugcccaaaua uucgcuuuuu gaacuggaga auggccguaa gcggauguug     3780

gccuccgcug gugaacucca gaaggggaac gagcuggcac uaccuucuaa auacgugaau     3840

uuccuguauc uugccagcca uuacgagaag cucaaaggau caccagaaga uaacgagcag     3900

aagcagcugu ucguagaaca acacaaacau uauuuggacg agaucauuga acagauaagu     3960

gaguuuucca agagagugau cuuagcagau gccaaucugg acaaaguccu cucugcuuac     4020

aacaagcaca gggauaagcc cauucgcgag caggcggaaa acaucauuca ccuguucacg     4080

cuuaccaauc ugggcgcccc ugcagcuuuu aaauauuucg acacaacuau cgaucgaaag     4140

agauacacca gcacaaaaga gguguuggac gccacucuaa uacaucaauc caucaccggu     4200

cuguacgaaa cgcggauuga ucucucacag cugggcgggg acaagaggcc agccgcuacc     4260

aaaaaggcag gacaggccaa gaaaaagaaa uaaggacuag uaauaaggaa acuguaugaa     4320

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4380

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4497


<210>  3523
<211>  5280
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3523
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc aaacuucaag auucugccaa uagccaucga ccucggaguu      180

aaaaauaccg guguguuuag cgcguucuac cagaagggca caucccuuga gaggcuggau      240

aacaagaaug ggaaaguaua ugaauugucu aaggacucau acacuuuacu gaugaacaau      300

cggacggcuc gccgacauca acguagagga auugauagga aacagcuagu gaagcggcuc      360

uuuaaacuga ucuggaccga gcagcuuaac cuggaauggg acaaggauac acagcaagcc      420

auuaguuucu uguuuaaucg cagaggcuuc agcuuuauca cugacgguua uuccccggag      480

uaccucaaca uaguccccga acaggugaag gcaauccuga uggauauuuu cgacgauuau      540

aauggggagg acgaucugga cucuuaccuc aaauuagcca ccgagcagga aucgaagauc      600

ucagagauuu auaacaaacu gaugcagaag auccuugaau uuaaauugau gaagcugugc      660

acagauauaa aagacgacaa gguuagcacc aagacucuaa aagagauuac aaguuacgag      720

uucgaacucc uggcugauua ccuugcaaau uauuccgagu cucugaagac ccaaaaauuu      780

agcuacacgg acaagcaggg aaacuugaaa gaacucuccu auuaccacca ugauaaguau      840

aauauccagg aguuccugaa gaggcacgcc acuauuaacg accggaucuu agauacccug      900

cucacagacg aucuugacau cuggaauuuu aacuucgaaa aauucgauuu ugacaagaac      960

gaggagaaac ugcagaauca agaagauaag gaccauauuc aggcucacuu gcaccauuuc     1020

guguuugccg ucaacaaaau aaagucagag auggcgagug gcggccgcca ccgaagccag     1080

uacuuccaag aaaucacuaa ugugcuagac gagaacaauc aucaggaagg guaucugaag     1140

aacuuuugug agaaucucca caacaaaaag uacucuaauc uguccguuaa aaaccuugua     1200

aaucugauug gaaacuuguc aaaucucgag cugaagccuu uaagaaaaua cuucaacgau     1260

aagauccacg caaaggccga ccauugggau gaacagaaau uuaccgagac auauugccac     1320

uggauacugg gugaauggag ggugggcguc aaggaccagg auaaaaagga cggggcuaaa     1380

uacagcuaua aggaucuaug uaaugagcuc aaacaaaagg ugaccaaggc cggacuggug     1440

gacuuccuuu uggaacugga uccaugccgg acgauucccc cuuaccucga caacaacaau     1500

cguaaaccac ccaaguguca gagucugauc cuuaacccua aauuucugga uaaucaguau     1560

ccaaacuggc agcaauacuu gcaggagcuc aagaaacugc agucuaucca gaauuauuua     1620

gacuccuucg agacugaucu gaagguccua aagagcucga aagaccaacc guacuuuguu     1680

gaauacaagu cuuccaacca gcagauugca ucaggccagc gcgacuauaa agaucucgac     1740

gcuagaaucc ugcaauucau uuuugauagg gugaaggcca gugacgagcu uuugcugaau     1800

gaaauauacu uccaggcgaa aaagcucaag cagaaagcca gcuccgagcu ggagaaguua     1860

gaaucuagca aaaagcugga ugagguaauc gcaaacucac aacuuaguca gauuuugaaa     1920

ucccagcaua caaaugguau cuucgaacag gggaccuuuc uccaccuggu gugcaaguau     1980

uacaagcaac ggcagcgagc ucgcgacagc agacuauaua ucaugcccga guacagguau     2040

gauaaaaagc ugcauaaaua caacaauacu ggacgguucg acgaugacaa ccagcuccug     2100

acauacugua aucacaagcc ucgccagaaa agauaucaac uuuugaacga ucuggccggc     2160

gucuuacagg ugucuccaaa uuuucucaag gacaaaauug gcucagauga cgaccuguuc     2220

auaaguaagu ggcuuguuga acacaucagg ggguuuaaga aagcaugcga ggauagccug     2280

aagauucaga aagacaaccg uggauugcuc aaccauaaga ucaauauugc ccggaacacc     2340

aaagguaagu gugagaagga aaucuucaau cugauaugca aaaucgaggg cuccgaagau     2400

aagaaaggga acuacaagca cggacuagca uaugagcugg gcgugcuccu guuuggugaa     2460

cccaaugagg ccucuaaacc ugaguucgac cgaaagauua agaaauuuaa cucgaucuac     2520

agcuucgcuc agauucaaca gaucgcguuu gcagaaagaa aggggaaugc caacacgugu     2580

gcugucugcu ccgccgauaa ugcacauagg augcagcaaa uaaaaauuac cgagccagug     2640

gaagacaaca aggauaaaau cauucuuuca gccaaggcuc agcgcuugcc cgccaucccg     2700

acacggauag uagacggcgc aguuaagaaa auggcgacua uccuggcuaa gaauauugug     2760

gaugacaacu ggcagaauau caaacagguc uuaagugcca agcaccaacu ccacauuccu     2820

aucauaaccg agucuaacgc auucgaauuu gagcccgccc uggcugaugu gaaaggaaag     2880

agccuuaagg acagaaggaa aaaggcccug gagcgcauuu ccccagaaaa uaucuucaaa     2940

gacaagaaca accgaaucaa agaguucgca aagggcauuu cagcuuauag cggggccaau     3000

cuaacagaug gagacuuuga uggugcgaaa gaagaacucg accauaucau uccucggucu     3060

cacaagaagu acggcacucu gaacgaugag gccaauuuga uaugcgucac ccguggggac     3120

aacaaaaaua agggaaacag aaucuucugu cugagggauu uagcugacaa uuauaaacuc     3180

aagcaguuug aaacaaccga ugaccuggag auugagaaaa agaucgcaga uacuauuugg     3240

gacgccaaca agaaagauuu caaguuuggc aauuaccgcu ccuucaucaa ccuuacgccc     3300

caggagcaga aggcuuuucg gcaugcccug uucuuggcag acgaaaaucc aauaaaacaa     3360

gcggugauca gagccauuaa caauaggaac cgaacauuug uuaaugguac ccagcgcuac     3420

uucgcugagg ugcucgccaa caauaucuau cugcgggcua aaaaggagaa ccuaaacacu     3480

gacaagauua guuuugauua cuucgggauc cccaccauag gcaauggaag aggcauugcu     3540

gaaaucaggc agcuguauga gaaaguagac agcgauaucc aggccuacgc aaagggggac     3600

aaaccucaag ccucuuauuc ccaccuuauu gaugcuaugc ucgcguucug cauagccgca     3660

gacgaacauc guaacgaugg aucaaucggu cuggagauug acaagaauua caguuuguau     3720

ccacuggaua aaaacacagg cgaagucuuu accaaggaca ucuucagcca gauuaagauc     3780

acugauaaug aguuuucgga caaaaaguua gugcgcaaaa aggcuauaga gggguucaac     3840

acacaccggc agaugacgag agauggaauc uacgccgaaa auuaccuccc gauucugauc     3900

cacaaagagc uuaacgaagu uaggaagggc uauaccugga aaaauuccga ggaaauuaag     3960

aucuuuaagg guaaaaagua cgacauacag caacugaaca auuuggugua uugucucaaa     4020

uucgucgaca agcccauuuc uaucgauauc cagauuagca cucuggagga gcuacgaaac     4080

auccugacaa ccaauaacau agccgcuaca gcagaauacu auuacauuaa ucuuaaaacu     4140

cagaagcucc augaguauua caucgaaaac uacaacaccg cccuggggua uaagaaauac     4200

ucaaaggaga uggaguuuuu gcggaguuua gcguaucgcu ccgaaagagu gaaaauuaag     4260

ucuaucgacg auguaaaaca agugcuggac aaggauagca auuucauaau uggcaagauc     4320

acgcucccuu uuaaaaagga guggcagagg cuguaccggg aauggcagaa caccacaauc     4380

aaagacgauu augaguuccu uaaguccuuu uucaauguua aaucaauuac uaagcugcac     4440

aagaaagucc gaaaggacuu uagcuugcca aucaguacca acgaaggaaa auuccuagug     4500

aagagaaaaa caugggauaa uaacuucauu uaccagauac ugaaugacuc ugauucccgc     4560

gcugacggca cuaagcccuu uaucccugcc uucgacauua gcaaaaacga gaucguggag     4620

gcaaucauug auucguuuac cucaaagaau auauucuggc ucccaaagaa caucgaacug     4680

caaaaagucg acaauaagaa cauuuuugcc aucgauacgu cuaaaugguu cgagguugaa     4740

acacccagug accuuaggga uauugggauc gcuaccauac aguauaagau cgacaauaac     4800

ucccguccga aagugcgggu gaaguuagau uacguaauug acgaugacag caagaucaau     4860

uacuuuauga accauucucu gcucaaaagc agauauccug auaaggucuu ggagauucug     4920

aaacagucca cuaucauaga guucgaauca aguggauuua acaagacaau uaaagagaug     4980

cucggcauga agcuggcagg uaucuacaau gaaaccagca acaauaagag gccagccgcg     5040

acuaaaaagg cugggcaggc caaaaagaaa aaguaaggac uaguaauaag gaaacuguau     5100

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     5160

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     5220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     5280


<210>  3524
<211>  3552
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3524
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc aaagaggaac uacauucugg gacucgacau agguaucaca      180

ucaguuggcu augggauuau cgauuacgag acacgggacg ugauugaugc cggaguacgc      240

cuuuucaaag aagcgaaugu ggagaacaau gaaggccgac guuccaagag aggugcuagg      300

cggcugaagc gcagaaggcg gcaucgcauc cagcgaguca aaaaguuguu auuugacuau      360

aaccugcuaa cugaucacuc ugagcucuca gggauaaauc cauacgaagc cagagugaaa      420

ggacugaguc aaaaacuuag cgaggaggag uucuccgcag cccuguugca ucucgcuaaa      480

aggcggggcg uucacaacgu gaaugaaguc gaagaggaca cgggcaacga gcugucuacc      540

aaggaacaga ucucgcguaa uucaaaggca cuggaggaaa aauauguggc cgagcuccag      600

uuagaacgcc ugaagaaaga uggggaggua agaggaagca uuaacagguu uaagacaagu      660

gacuacguua aagaggcuaa gcagcuuuug aaagugcaaa aggccuauca ucagcuggau      720

caguccuuca ucgacacuua cauugaucua cucgaaaccc ggcgaacaua cuaugagggu      780

ccgggcgaag ggucucccuu uggauggaag gacaucaaag agugguacga aaugcugaug      840

ggccacugca ccuauuuccc ugaggagcuu cgcagcguca aguacgcgua uaaugcagau      900

cuguacaacg ccuugaauga ccucaacaau cuggugauaa cuagagacga aaacgagaaa      960

uuagaauauu acgagaaguu ucagauuauc gaaaacgugu ucaaacaaaa gaagaaacca     1020

acacugaagc agauugcuaa agagauccuc gucaaugagg aagauaucaa ggguuacagg     1080

guuaccucca cggggaaacc cgaguuuacu aaccuuaagg uguaucacga cauuaaggau     1140

auaaccgccc ggaaagaaau cauugagaau gcagagcugu uggaccagau cgcuaagaua     1200

cuaacaauuu accagucaag ugaagauauc caagaggaac ugacuaaccu caauagcgag     1260

cugacccagg aagagaucga gcagauuucu aaccuuaaag gauauacagg cacccauaau     1320

cuguccuuga aggccaucaa ccucauucug gacgaauuau ggcacacgaa ugauaaccaa     1380

auagcgaucu ucaaucgccu gaaacuugua ccuaagaagg uggaccucuc acagcagaaa     1440

gagauuccaa cuacacuggu cgaugacuuu auccuuagcc ccgugguuaa gagaaguuuc     1500

auccagucua uuaaagugau aaacgccauc auuaagaaau acggcuugcc uaaugauauc     1560

auuaucgaac uggcaaggga gaagaacucc aaagacgcuc aaaagaugau aaaugaaaug     1620

cagaagcgua accggcagac caacgagcga aucgaggaaa uuaucagaac uacagggaaa     1680

gagaaugcca aguaucucau ugaaaagauc aagcugcaug acaugcagga gggaaaaugu     1740

cuuuacagcc uggaagcuau accauuggag gaucuccuga acaauccguu uaacuaugag     1800

gucgaccaca uuauccccag gucggugucu uucgauaauu ccuucaacaa uaagguauua     1860

guuaagcaag aagagaacuc aaagaagggu aaucgcaccc cuuuucagua ccugaguagc     1920

uccgacucua aaauuagcua cgaaacguuc aagaaacaca uccuaaaccu cgccaagggc     1980

aaggggcgga uaucaaaaac caagaaagag uaucugcuug aggaaagaga uaucaauagg     2040

uuuagugugc agaaggacuu cauuaaccgc aauuuggucg auacacgaua cgcaacucgg     2100

ggacugauga accuccugcg uuccuauuuu agagugaaua acuuagacgu caaagugaaa     2160

agcaucaacg gcgguuucac cucuuuucug aggcgcaagu ggaaauucaa gaaagagcgg     2220

aauaaggggu acaaacauca cgccgaagau gcucuuauua ucgcgaacgc agacuuuaua     2280

uucaaggagu ggaaaaaguu ggauaaggcc aaaaagguua uggaaaauca gauguuugag     2340

gagaaacaag cugaaucaau gccagagauu gaaacagagc aggaauauaa ggagaucuuc     2400

aucacucccc aucagauuaa acacaucaag gacuuuaagg auuacaaaua uagucauaga     2460

guggacaaga aaccuaacag ggagcucauu aaugacaccc uguacagcac acgaaaggau     2520

gacaaaggca acacccuaau aguaaauaac cugaauggac ucuacgauaa ggacaacgau     2580

aagcugaaaa agcuaaucaa uaaaucccca gaaaaguugc ugauguauca ccacgacccc     2640

cagacuuacc aaaaauuaaa gcucauuaug gagcaguaug gcgaugaaaa gaacccgcug     2700

uacaaauauu acgaggaaac ggggaauuau cuuacaaagu acucuaaaaa ggacaacgga     2760

ccugucauca aaaagauuaa guacuauggu aauaaacuga acgcccauuu ggauaucacc     2820

gacgauuacc ccaacucgcg caauaaggug guuaaacuca gccugaagcc auaucgguuc     2880

gacguguacc uagauaacgg cgucuauaaa uucgugacug uaaagaaucu ggacgugauc     2940

aaaaaggaga acuacuauga gguuaauucc aagugcuacg aagaggccaa aaagcucaaa     3000

aagauaucaa accaggcaga auuuauugcu aguuucuaca auaacgaccu gaucaaaauu     3060

aauggggagc uuuauagagu caucggagug aacaaugauu ugcugaacag gauagaggug     3120

aauaugauug acaucaccua ccgugaauau uuagagaaca ugaaugauaa gcgcccuccc     3180

cggaucauua agacaauagc cucuaaaacc caaagcauca agaaauacuc cacugacauu     3240

cucggcaacc uguaugaagu caagagcaaa aagcacccac agaucauuaa gaaagguaag     3300

agacccgcag cgaccaaaaa ggcugggcag gccaaaaaga agaaauaagg acuaguaaua     3360

aggaaacugu augaaugucu gugggcagga agugaagaga uccuucugua aagguuuuug     3420

gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua aaucugguag aaaaaugaga     3480

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaagaa uu                                                         3552


<210>  3525
<211>  4620
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3525
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc acuguucaac aagugcauua uaaucagcau uaaucucgac      180

uuuuccaaca aagagaagug uaugaccaag ccauacucua ucggacuuga uauugguaca      240

aauucaguug gcugggccgu gaucacugac aacuauaaag uaccgaguaa gaaaaugaag      300

gugcugggga auacgagcaa aaaguacaua aagaaaaacu uguuaggagu ccugcuauuc      360

gauuccggca ucaccgcgga agguaggcgg cucaagcgca cagcucgacg uagauauacu      420

aggcggcgca auaggauucu guaccuucag gagaucuuuu cuaccgaaau ggccacacug      480

gacgaugcau ucuuucaaag auuggacgau ucguuccucg ugcccgacga uaaacgggac      540

ucaaaguauc cuauuuuugg gaaccugguu gaggaaaaag uguaccauga ugaguuccca      600

accaucuauc aucugcgcaa guaccucgcc gacagcacua aaaaggcuga uuuacgacug      660

gucuaccuug cauuggccca caugauaaag uauagaggac acuuucugau ugagggcgag      720

uucaauagua aaaacaauga cauccagaag aacuuucagg acuuccuaga uacauacaau      780

gcuauuuuug aguccgaccu cucucuggaa aacagcaaac agcuugagga gaucgugaag      840

gauaaaaucu ccaagcugga aaagaaagac aggauuuuga aacucuuccc cggcgagaaa      900

aauucaggga uauucaguga auuucugaag uuaaucguag gaaaccaagc cgauuuccgg      960

aaaugcuuua accuggacga gaaggcgagc cuccauuucu cuaaggaauc cuaugaugag     1020

gaccuugaga cccuguuggg uuacauuggc gaugacuauu cagauguuuu ucuaaaagca     1080

aagaaacugu acgacgccau ccuccugagc ggguuccuua cggugacuga caaugaaacc     1140

gaggcuccuc ugaguucugc caugauaaag cguuauaacg aacacaaaga ggauuuggca     1200

cuccugaagg aauacauucg caauaucucc uuaaagacau acaacgaggu cuuuaaagac     1260

gauacuaaga auggauaugc uggcuacauc gacgguaaaa ccaaccagga ggauuucuau     1320

guguaccuga agaaucuacu cgccgaauuu gagggggcgg acuauuuccu ggaaaagauu     1380

gauagagagg acuuucuuag gaagcagcgg acauucgaua acggaagcau cccauaccag     1440

auucacuugc aagaaaugcg agccauacug gacaaacagg caaaguuuua ucccuuccuc     1500

gcuaagaaua aagagcgcau cgagaagauu cugaccuuca gaaucccuua cuacgugggc     1560

ccacuugcca ggggcaacuc ggauuuugcu uggucuaucc ggaaacgcaa ugaaaagauu     1620

acgccgugga acuucgagga cgucauagau aaagaauccu cagccgaggc auuuaucaau     1680

agaaugacaa guuucgaccu guauuugccc gaggaaaagg uucucccuaa gcauagccug     1740

uuauacgaga cuuuuaacgu guauaaugaa cugaccaaag uaagguucau ugccgagucc     1800

augcgugacu accaguuucu agauucuaag cagaaaaagg acaucgugcg gcucuauuuc     1860

aaagauaagc gaaaggucac ugacaaagau auuaucgaau accugcacgc uauauauggg     1920

uacgacggaa ucgagcuuaa ggguauugag aaacaauuua acagcucauu gaguacauac     1980

caugaucugc ucaacaucau uaaugacaag gaguuccugg augacuccag caacgaagcg     2040

aucauagaag agauuaucca caccuuaacg auuuuugaag auagagagau gaucaaacag     2100

aggcugucua aguucgagaa uauauuugac aagucagugc uuaaaaaguu gagucgccgg     2160

cacuauaccg gcugggggaa acucagcgca aagcugauca acggaauuag agaugaaaaa     2220

uccggcaaua caauccuaga cuaccugauu gacgauggua ucucuaacag gaauuucaug     2280

cagcucauac augacgaugc ccugucguuc aagaaaaaga uucaaaaggc ucagaucauc     2340

ggggacgagg auaaaggcaa cauuaaggaa guugugaaaa gccuuccagg aucccccgcc     2400

aucaagaaag gcauuuugca gucaauaaag aucgucgacg agcuggugaa gguaauggga     2460

gggcgcaaac cugaaaguau uguuguggag auggcacgag agaaucagua uacuaaccaa     2520

gguaagucua auagccagca gcgguuaaaa cgucucgaaa agucccugaa agagcuuggc     2580

ucaaagaucc ugaaggaaaa cauuccagcc aaauugagca agaucgauaa uaacgcucuc     2640

cagaaugaca gacuguaccu auauuaccug caaaacggga aagauaugua uaccggagac     2700

gaucucgaca uagacaggcu gucuaacuac gauaucgacc acauuauccc ccaggccuuu     2760

cuuaaggaua auuccauuga caacaaaguc uuggugagua gcgcaucuaa ucgcggcaag     2820

uccgaugacu ucccgucacu ggaggucgug aagaaacgga agacauuuug guaccaguua     2880

cucaaaagua agcugaucag ccagagaaaa uucgauaacc uuacuaaggc ggagaggggu     2940

gggcugcuac cugaagacaa ggcuggcuuu auacaacgac agcucguuga gacccgccag     3000

auuacaaaac auguggcccg gcuguuggau gaaaaguuca auaacaaaaa ggacgagaau     3060

aacagagcag uaaggaccgu caaaaucauc acucugaagu cgacguuagu gucccaguuu     3120

cguaaagauu ucgaacucua uaagguucgc gagauuaaug acuuucacca ugcccacgau     3180

gcuuaccuga acgccgugau agcaucugcc cuucugaaga aauaucccaa guuggagcca     3240

gaguucgucu acggagacua uccuaaauac aauagcuuuc gggaaagaaa gucagcuaca     3300

gaaaaagugu auuucuacag uaacaucaug aauauuuuca agaaguccau cucucucgcg     3360

gacggcaggg uaauugagcg accccugauc gaagugaacg aggagaccgg ggaaagcguu     3420

uggaauaaag aguccgaucu agccacuguc cggcgcgugc ugucauaccc acaagugaac     3480

gucguuaaga aaguggaaga acagaaccac ggacuugaca gagguaagcc caaaggccuc     3540

uuuaaugcua accugagcag uaagccuaag ccaaauucua acgaaaauuu ggugggggca     3600

aaagaguauc uggauccgaa gaaauacgga ggcuaugccg guauauccaa cagcuucgcu     3660

guauuaguca aggggaccau cgagaaaggc gccaagaaga aaauuacaaa ugugcucgaa     3720

uuucagggaa ucucgauucu ggacaggauc aacuaccgga aggauaaacu uaauuuccug     3780

uuggagaagg gcuauaaaga cauagaacuc auuaucgagc ugcccaagua cucacuauuu     3840

gagcugucug augggagucg aagaaugcuu gcauccaucc ucagcaccaa caauaaacgc     3900

ggagaaauuc auaagggcaa ccaaaucuuc cugucucaga aguuuguuaa auuguuauau     3960

cacgcgaaga ggauaagcaa uacuauuaac gagaaucauc guaaauacgu ggaaaaccac     4020

aagaaagagu ucgaggaacu guuuuacuau auccucgagu ucaaugaaaa cuacgucggu     4080

gccaagaaga acgggaaacu gcuuaauucc gcuuuucagu cauggcagaa ccacaguauu     4140

gacgagcugu guagcucuuu caucggaccu acaggcuccg aacggaaggg uuuguuugag     4200

cuaacgucaa gaggcagugc cgcagauuuc gaguuccugg gggugaaaau accaagguau     4260

cgcgacuaca cccccagcuc ccuccugaag gaugcuacuc uuauucauca aucuguuaca     4320

ggauuauaug aaacccgaau cgaccuggcc aaacucggcg aggggaagag accugcagcc     4380

acaaagaaag cuggacaggc gaagaaaaag aaauaaggac uaguaauaag gaaacuguau     4440

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     4500

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     4620


<210>  3526
<211>  3696
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3526
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc augguacgcc agccugaugu ccgcgcauca ccucaggguu      180

ggaauugacg uggguaccca uucuguaggc cuugcuacac ugcgggugga ugaccacggg      240

acuccaauag aguuguuauc agcccugagu cauauccacg auagcggagu cggcaaggaa      300

gguaaaaagg accacgauac gcgcaagaaa cuauccggga uugcacgacg ugccagaagg      360

cuccugcauc accggcgcac ccagcuucaa cagcuggacg agguguugag agaucucgga      420

uucccgaucc ccacaccugg cgaauuucug gaccugaacg agcagacuga uccauauagg      480

guuuggcggg ugcgcgcucg acucgucgaa gagaaguuac ccgaggaacu gagaggcccu      540

gcaauuucua uggccgugag gcauaucgcu cggcaccgug gguggcgcaa uccauacucg      600

aaaguagagu cacuuuugag ccccgccgaa gagaguccuu ucaugaaggc gcugagagag      660

aggauacuag caaccacagg agaaguucuc gacgauggua ucaccccagg ccaggccaug      720

gcucaagugg cccugacuca caacauuucc augcgggggc cggagggaau ccuuggcaaa      780

cugcaucagu cugacaaugc aaacgaaauu cgaaagaucu gcgcucgcca gggugucagc      840

cccgaugugu guaagcaguu gcucagagcc guguuuaaag cggacucccc uaggggguca      900

gccgucaguc ggguugcacc agacccccug ccuggacaag gcagcuuccg cagagcucca      960

aagugcgauc ccgaguuuca gagguuccgu auaauuucua ucguggccaa uuuacggauu     1020

uccgaaacaa aaggcgagaa ccgaccgcug accgcugacg agagaaggca cguagugacg     1080

uuucucacug aagauucaca ggccgaccuu accugggucg auguggcaga gaagcugggg     1140

guucaucgcc gggacuugag aggaacagcc gugcacacug augacgguga aaggagcgcu     1200

gcgcgcccuc ccaucgaugc aaccgaccga aucaugcggc agacaaaaau uaguucucua     1260

aagaccuggu gggaggaagc cgauuccgag caacguggcg cuaugauaag auaucuguac     1320

gaggacccaa cggacagcga augugccgag aucauugcag aacucccuga ggaagaucag     1380

gccaaacugg acucgcuuca ucugcccgcu gggagggccg cauauucucg cgaguccuug     1440

acugcgcucu cagaucacau gcuggcuaca accgacgauu uacacgaggc ccggaagaga     1500

cuguucggag ucgacgauag uugggcacca cccgccgaag cuaucaaugc cccugugggc     1560

aacccaagcg uagacaggac ucuaaagaua guuggucgau accucuccgc aguggagucu     1620

auguggggga caccggaagu cauucaugug gagcacgucc gcgauggcuu uaccagcgag     1680

cggauggcug acgaaagaga uaaagccaau aggcgucgcu auaacgacaa ucaggaggcg     1740

augaagaaaa uccaacggga uuacggaaag gaaggcuaca ucucaagagg ggacauugug     1800

aggcuggacg cccuugaguu gcagggaugc gcuugucugu auugcgguac gaccaucggc     1860

uaccauacau gucagcucga ucacauuguu ccccaggcag ggccuggaag uaacaaucga     1920

cggggcaacc ugguggccgu augcgaacgc uguaauagau ccaaaagcaa cacuccauuc     1980

gcugucuggg cccaaaagug cgguauaccc cacguggggg uuaaggaggc aaucggcagg     2040

gugcggggau ggcgaaaaca gaccccuaac acaucuucag aggaccuuac uagacugaag     2100

aaagaaguca uugcgcgcuu gaggcguacc caggaggauc cagaaaucga cgagcggagu     2160

auggaaagcg uggccuggau ggcuaaugag cuccaucaca gaaucgccgc agcuuauccc     2220

gagacaaccg uaauggugua caggggcucc auuacugccg cagcccgcaa ggcugcgggg     2280

auagauucuc gaaucaaccu gauuggagaa aaagguagaa aggaccggau cgauaggcgc     2340

caucacgccg uugacgcauc ggucguggcu uuaauggagg ccagcguggc caagacgcug     2400

gcugaacguu ccucacuaag aggcgagcag cggcucacag ggaaagaaca aaccuggaag     2460

caguauacug gaaguaccgu cggcgcaagg gagcauuuug agauguggcg cggucacaug     2520

cugcaccuua cagaauuguu caaugagcga cuggccgaag auaaaguuua cgugacccag     2580

aacauuagac uccggcuguc ugacgggaau gcgcauacug ugaacccgag caaguuagua     2640

ucccacaggc ugggcgaugg acuuacaguc cagcaaaucg accgcgcuug uacgccugcc     2700

uuguggugcg cacucaccag agagaaagau uuugacgaga agaauggccu gccagccagg     2760

gaagaccggg cuauacgugu gcaugggcac gagaucaagu caagcgauua uauucagguu     2820

uucucuaaac gcaagaaaac ugacuccgau cgagacgaaa cacccuucgg agcaaucgcc     2880

gugagaggcg guuuugucga gauugggccu aguauccauc acgcgaggau auaccgggug     2940

gaaggaaaga aacccguuua cgcuaugcua cgcguguuca cccacgaucu gcucagccag     3000

agacauggcg accuguuuuc ugccguaauu ccaccucagu ccaucucaau gaggugcgca     3060

gagcccaaac uucggaaagc cauuacaacu gguaacgcua ccuauuuggg cugggucgug     3120

gugggggaug agcuggaaau caaugucgac aguuucacga aguacgccau aggacgauuu     3180

uuagaggauu ucccaaacac cacaagaugg cgcaucugug gcuaugacac uaauagcaag     3240

cucacccuga aaccgauugu ucuugcagcu gaagggcugg agaacccuuc guccgccgug     3300

aaugaaaucg uagaguugaa gggauggagg guggcgauua acguccucac aaaagugcac     3360

cccacuguug ugcggcguga ugcccugggc agaccaaggu acucuagccg cucaaaucua     3420

cccaccaguu ggacgaucga gaagcggccu gcagcuacaa agaaggccgg ucaagcuaag     3480

aaaaagaaau aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     3540

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     3600

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3696


<210>  3527
<211>  4557
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3527
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc aaccaagcca uacagcauug gacuggacau agguacaaac      180

uccguuggcu gggccgugac uacggauaau uauaaaguac cgucuaagaa gaugaaagug      240

cucgggaaca ccucaaagaa auacaucaag aaaaaucuuc ugggagucuu guuauucgac      300

aguggcauua cagcggaggg uaggcggcug aagcgcacug cucgacguag auauaccagg      360

cggcgcaaca gaauccuaua ccuccaggaa auuuuuagca cagagauggc cacccuggau      420

gacgcauucu uucaaaggcu ugaugacucc uuccuggugc ccgaugacaa gcgggauucu      480

aaauauccua ucuuugggaa uuugguugaa gagaaggccu accaugacga guucccaacu      540

auauaucacc uccgcaaaua ccuggcugau ucgacaaaga aagcagaccu gcgacucgug      600

uacuuagccc uggcucauau gaucaaguau agaggacacu uucuuauuga gggcgaguuc      660

aacucaaaga auaacgauau ccagaagaau uuucaggacu ucuuggacac cuacaacgcc      720

auuuuugaaa gcgaucugag ucuagaaaau uccaagcagc ucgaagagau cgucaaagac      780

aagauaucua aacuggagaa gaaagauagg auucuuaagc uguuccccgg cgaaaagaac      840

agcgggaucu uuuccgaguu cuugaaacuc auugugggaa aucaagcgga cuuccggaag      900

ugcuuuaacc uggaugaaaa agcaucauua cauuucagua aggagagcua ugacgaagau      960

cuggagacgc uccuugguua caucggcgac gauuauucug acguauuucu gaaagccaag     1020

aaguuguacg augcuauccu acuguccggg uuccucacug uuaccgacaa ugagacagaa     1080

gccccucugu caagcgcaau gauuaaacgu uauaacgagc acaaggaaga ccuugcucug     1140

uugaaagagu acauacgcaa caucagucuc aagacuuaca augaaguguu uaaagaugac     1200

accaagaacg gauaugccgg cuacauugau gguaagacaa aucaggagga cuucuauguc     1260

uaccugaaaa aguuacuggc ggaguuugaa ggggccgauu auuuccuaga gaaaaucgac     1320

agagaagauu uucucaggaa gcagcggacc uucgacaacg gaucuauacc auaccagauu     1380

caccuucaag agaugcgagc aauccuugau aaacaggcua aguuuuaucc cuucuuggcc     1440

aaaaauaagg aacgcaucga gaagauucug acguuuagaa ucccuuacua cgugggccca     1500

cucgcuaggg gcaacuccga cuucgccugg agcauucgga aacgcaauga gaagauaacu     1560

ccguggaacu ucgaagaugu gaucgacaaa gagucgucug cagaagccuu uauuaauaga     1620

augacauccu ucgaucugua ucuucccgag gagaaggucc ugccuaaaca uucauugcuc     1680

uacgaaaccu uuaacguuua uaaugagcug acuaagguga gguucaucgc ugaaaguaug     1740

cgugacuacc aguuuuuaga cagcaagcag aaaaaggaua ucguacggcu guauuucaaa     1800

gacaagcgaa aagugacaga uaaggacauu auagaguacc uacacgcgau cuauggguac     1860

gauggaauug aacucaaggg uaucgagaaa caauuuaacu ccucucugag caccuaccau     1920

gaccuuuuga auauuaucaa cgauaaggag uuccuggacg auucaaguaa ugaagcaaua     1980

aucgaggaaa uuauccacac gcucaccauu uuugaggaca gagaaaugau caaacagagg     2040

cuguccaagu ucgagaacau auuugauaaa agcgucuuaa agaagcuguc ucgccggcac     2100

uauacaggcu gggggaaacu uucagccaag uugauuaacg gaaucagaga cgagaaaagu     2160

ggcaauacua uucucgauua ccugaucgac gacgguauaa gcaacaggaa uuucaugcag     2220

cuaauccaug augacgcucu guccuuuaag aaaaagauuc aaaaagccca gaucauuggg     2280

gaugaagaca agggcaacau caaggaggug guuaaaucuc ucccaggauc gcccgcaaua     2340

aagaaaggca uucugcagag caucaagauc guggaugaac uugucaaagu gaugggaggg     2400

cgcaagccug aguccauugu aguugaaaug gcccgagaga aucaguauac caaccaaggu     2460

aagucaaaua gucagcagcg guugaaacgu cuggagaagu cuuuaaaaga acucggcagc     2520

aagauccuga aagagaacau uccagcuaag cuguccaaga uagacaauaa cgcccugcag     2580

aaugauagau uguaccucua uuaccugcaa aacgggaaag acauguauac aggagaugac     2640

cuagauaucg acaggcuguc aaauuacgac auugaucaca ucauucccca ggcauuccuc     2700

aaggacaaca gcaucgauaa uaaagugcug gucucuuccg cgaguaaccg cggcaagagc     2760

gacgaugugc cgucucuuga agucgugaaa aagcggaaga cuuucuggua ccaguugcug     2820

aaauccaagu uaauaucaca gagaaaauuu gacaaccuca ccaaggcuga gagggguggg     2880

cugaguccug aggauaaagc cggcuucauc caacgacagc uuguugaaac acgccagauu     2940

accaagcaug uggcacggcu gcuagacgag aaguuuaaua acaaaaagga ugaaaauaac     3000

agagccguaa ggacugucaa aaucauuacg cucaagagca cacugguguc gcaguuccgu     3060

aaagacuuug aguuguauaa gguucgcgaa aucaaugauu uccaccaugc ucacgacgcc     3120

uaccugaacg caguggucgc uuccgccuua cucaaaaagu aucccaagcu ggagccagag     3180

uuuguguacg gagauuaucc uaaauacaau ucuuuccggg aaagaaagag cgcgaccgag     3240

aaaguauauu uuuacucaaa cauaaugaau auuuucaaga aaaguaucuc ccuugccgac     3300

ggcaggguga ucgaacgacc ccugauugag guuaacgaag agacugggga gucugucugg     3360

aauaaggaaa gcgacuuggc uaccgugcgg cgcgugcucu ccuacccaca agucaacguu     3420

gugaagaaag uggaggaaca gaaucacgga cuggauagag guaagcccaa aggccuauuu     3480

aacgcaaauc ugucaagcaa gccuaaacca aacaguaacg agaaucuugu aggggccaag     3540

gaauaucucg acccgaagaa auacggaggc uaugcuggua uaucuaacuc cuucacaguc     3600

cuggugaagg ggaccaucga gaaaggcgcc aagaaaaaga uuacuaaugu uuuggaguuc     3660

cagggaauca gcauucugga uaggaucaac uaccggaagg acaaauuaaa uuuucuccug     3720

gaaaagggcu auaaagauau agagcuuauc auugaacugc ccaaguacuc guuguucgag     3780

cucucagacg ggucucgaag aaugcuggca aguauccuau ccacaaacaa uaaacgcgga     3840

gagauucaua agggcaacca aaucuuucug agccagaaau ucgugaaacu ucucuaucac     3900

gcgaagagga uaucuaauac gauuaacgaa aaucaucgua aauacgucga gaaccacaag     3960

aaagaauuug aggaacuguu cuacuauauc uuggaguuua augagaacua cgugggugcc     4020

aagaaaaaug ggaaguuacu gaacagcgcu uuccaguccu ggcagaauca cucaaucgau     4080

gaacucugua guagcuuuau uggaccuacc ggcucugagc ggaagggucu guucgaacuu     4140

acuuccagag gcucagccgc agacuuugag uuccuggggg uuaaaauccc aagguaucgc     4200

gauuacacac ccaguagcuu gcuaaaggac gcuacccuga uacaucaauc cgugacagga     4260

cucuaugaaa cucgaauuga ucuggccaaa cuuggcgagg ggaagagacc ugcagccacc     4320

aaaaaggcug gacaggcgaa gaaaaagaaa uaaggacuag uaauaaggaa acuguaugaa     4380

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4440

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4557


<210>  3528
<211>  3639
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3528
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc agccgcguuc aagccaaaca gcauuaauua cauacuggga      180

cucgacaucg guauugcuuc cguuggcugg gccauggugg agaucgauga agaggaaaac      240

ccgauuaggc uuaucgaccu ggggguacgg guguuugagc gcgcagaagu ccccaaaacc      300

ggagauucuu uggccauggc ucgacguuua gcaagaucag ugaggcggcu gacacgcaga      360

agggcccauc ggcuacuccg cacucgaaga cugcuuaaga gggagggcgu ucugcaggcu      420

gccaauuucg acgagaacgg uuugauaaag agucucccua auacgccaug gcaacugcgg      480

gcggcagccc uggaucguaa acucaccccc uuagaaugga gcgcugugcu gcuucacuug      540

aucaagcauc gcggguaucu gucccagagg aaaaacgagg gagaaacagc cgacaaggag      600

cuaggcgcac uccugaaagg cgucgcuggg aaugcccacg cgcuucagac uggagauuuu      660

aggaccccug ccgagcuggc auugaacaag uucgaaaagg agucugguca uauucggaau      720

cagcgaucgg acuacucaca cacauuuagc cgcaaagauc uccaagcuga acugaucuua      780

cuguucgaga agcagaaaga auuuggcaac ccacacguga gugggggacu caaggagggc      840

auugagaccc uucugaugac ucagagaccc gccuuguccg gugacgcugu acagaaaaug      900

cuagggcauu gcacauucga accugccgag ccaaaggcag ccaaaaauac cuauacggcu      960

gaaagguuua ucuggcugac uaagcucaac aaucugcgga uacuugagca aggaucugaa     1020

cgcccgcuga ccgauacaga gagagcgacu uugauggacg agcccuacag aaagagcaaa     1080

cucaccuaug cacaggcccg uaagcuguua ggccuggaag auacagcuuu cuuuaaaggc     1140

cuacgguacg ggaaggacaa cgccgaggca uccacccuca uggaaaugaa agccuaucac     1200

gcuauuucac gagcccugga gaaggaagga cuuaaggaca aaaagagucc uuugaaucug     1260

agcccagagc uccaggauga gaucgguacg gcauucucuc uguuuaaaac ugacgaagau     1320

auuacaggca gacuuaagga caggauccag cccgagaucc uggaagcguu gcucaaacau     1380

auuuccuucg auaaguucgu ucaaauauca cugaaggcuu uacgccggau cgugccucug     1440

auggagcagg ggaaaagaua cgacgaggcc ugugcagaaa uuuacggaga ucacuauggc     1500

aagaaaaaca ccgaggaaaa gaucuaccua ccacccauac cggccgacga gauuaggaac     1560

ccugucgugc uccgcgcucu gagccaggcc cgaaaaguga ucaauggugu cguucggcgu     1620

uaugggaguc ccgcaagaau ccacauugaa acugcuaggg aggugggcaa gucuuuuaag     1680

gaucgcaaag agaucgaaaa gcggcaagag gaaaacagaa aagacaggga gaaggccgcg     1740

gccaaauucc gagaauacuu uccaaauuuc guaggagagc cuaaguccaa agauauucuu     1800

aaguugcgcc uguaugagca gcagcauggc aagugccucu acagcgggaa agaaauaaac     1860

cugggacggu uaaaugagaa ggguuaugug gaaaucgacc acgcucugcc cuuuucgaga     1920

acaugggacg auucuuucaa caauaaaguc cuuguguugg gcuccgagaa ccagaauaag     1980

gggaaccaaa ccccauacga auacuuuaau ggaaaagaca acucaaggga guggcaggag     2040

uucaaggcac guguugaaac gagucgcuuu ccccggagca agaaacagag gauucuccug     2100

cagaaguucg augaggacgg cuuuaaagaa agaaaucuaa acgauacccg auaugugaau     2160

cgguuccugu gucaauuugu cgccgaccgc augagacuca cagguaaggg gaaaaagagg     2220

guguucgcuu ccaacggcca gaucacuaac cugcuucggg gauucugggg cuugcgaaag     2280

guaagagccg agaaugaucg ccaucacgca cuggacgcgg uuguggucgc cugcucuacc     2340

guggcuaugc agcagaaaau cacaagguuu guccguuaca aggagaugaa cgccuucgau     2400

gggaaaacua uugacaagga aaccggagag guguuacauc agaaaacaca cuuuccucag     2460

ccaugggagu ucuuugcaca ggaaguuaug auacgggugu ucgguaagcc ggauggcaag     2520

cccgaauuug aggaggcuga cacccucgaa aaacugagaa cucuucuggc cgagaaguug     2580

agcucaaggc cugaagcagu acacgaguau gucacgccac ucuucgugag ucgcgccccc     2640

aaucgaaaaa uguccgggca gggacauaug gaaacaguua agagcgcuaa aagacuggau     2700

gagggcgugu cuguccuacg ggugccucug acccaacuca agcugaaaga ccuugagaag     2760

augguaaaca gggaacgcga gccaaaguug uacgaagcgc ugaaagcccg uuuagaggca     2820

cacaaggacg aucccgcuaa agccuuugcc gaaccguucu auaaguacga caaagcuggu     2880

aauagaacuc aacaggugaa ggcaguucgg gucgagcaag ugcagaagac cggggugugg     2940

gucaggaacc auaauggcau cgccgauaac gcgacaaugg uucgcgugga cguguuugag     3000

aaaggagaua aguauuaccu cguaccuauu uacucauggc aggucgcuaa aggcauccug     3060

ccagaccgag ccgugguuca ggggaaggau gaagaggacu ggcaacuuau ugaugacagu     3120

uucaauuuua aauucagccu gcaccccaac gaucuagugg aagucaucac caagaaggca     3180

agaauguucg gauauuuugc cuccugucac cggggcacug guaauauaaa caucaggauu     3240

caugaccucg aucacaaaau cgggaagaau ggcauucugg agggaaucgg ugugaaaaca     3300

gcuuugucuu uccagaagua ccagauagac gagcugggca aagaaauucg cccuugcaga     3360

uuaaagaaga ggcccccagu ucggaaacgu ccugcagcca cgaagaaagc ggggcaggcu     3420

aagaaaaaga aguaaggacu aguaauaagg aaacuguaug aaugucugug ggcaggaagu     3480

gaagagaucc uucuguaaag guuuuuggaa uuaugucugc ugaauaauaa acuuuuuuga     3540

aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            3639


<210>  3529
<211>  4395
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3529
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc aaagaaacca uacaccauug gacuggacau agguacaaac      180

agcguuggcu gggccgugcu cacugaucag uaugaccuug uaaagaggaa gaugaaaauc      240

gcgggggauu ccgagaagaa acaaauuaag aaaaauuucu ggggagugcg gcuguuugac      300

gaaggccaga cggcugccga ucgccgaaug gcacguaccg ccagaaggcg gaucgagcgc      360

agaaggaacc ggauuucuua cuugcagggu aucuucgcug aagagauguc aaagacagac      420

gcaaacuuuu ucugccgcuu aagugauagc uuuuaugucg acaaugaaaa gcgaaauucc      480

agacauccgu ucuuugccac uauagaggag gaaguggagu accacaaaaa cuaucccacc      540

aucuaccauc ugagggagga gcuaguuaau ucuucggaaa aggcugaucu ccggcuggug      600

uaucuugccc uggcgcacau uaucaaauac cgugggaacu ucuugauuga aggagcacuc      660

gacacacaga auaccucagu cgauggcauc uacaagcaau uuauacagac uuauaaccag      720

guguucgcca gcggcauuga ggacgggagu cugaaaaagc uggaagauaa uaaagacgua      780

gcuaagaucc ucguugagaa ggugacacgc aaagaaaagu uagagaggau ucugaaacuu      840

uacccuggag agaaguccgc cgguauguuu gcacaguuca ucucuuugau cgucggcagc      900

aaagggaacu uucaaaagcc auucgaccug auugaaaagu ccgauauaga gugugcuaaa      960

gacucauaug aagaggaucu agaaagucuc cuggcccuua ucggagacga guacgcggag     1020

cuguucgugg ccgcaaagaa ugcuuauagc gccguggucu ugucuuccau uaucaccguu     1080

gcugaaacgg agacuaacgc caaacucuca gcaagcauga uagaaagguu ugauacccau     1140

gaggaagacc ugggcgaguu aaaggccuuc auuaaacugc accuccccaa gcacuacgag     1200

gaaaucuuua guaauacaga gaagcauggu uaugcugggu acaucgaugg aaaaacuaag     1260

caggcggacu ucuacaaaua uaugaagaug acccuugaaa acauugaggg cgcagauuac     1320

uuuaucgcca aaauugagaa ggaaaacuuc cugcggaagc agcgaacauu ugacaauggc     1380

gcuauaccuc accaguugca ucuagaggaa cuggaggcca uccuccacca acaggcaaaa     1440

uauuacccau uccugaagga aaacuaugau aaaauuaagu cucuugugac cuuucgcauc     1500

cccuacuucg uagggccucu ggccaaugga caguccgagu uugcuugguu gacgagaaaa     1560

gccgacggug agaucaggcc auggaacauu gaagagaagg uggacuucgg caaaagcgca     1620

gucgauuuua uagaaaagau gacuaauaag gacacauauc ucccgaaaga gaacgugcug     1680

cccaagcacu cguuaugcua ccaaaaauac cugguuuaua augaacuaac caaggugcgg     1740

uacaucaacg aucaggggaa aacuucuuau uucuccggac aggagaagga gcagauuuuc     1800

aaugaccucu uuaagcaaaa acgcaagguc aaaaagaaag aucuggaacu uuucuugaga     1860

aacaugucac auguggagag uccuacaauc gaaggccugg aggacagcuu uaauuccucu     1920

uacagcaccu aucacgaucu ccugaaggua gguauuaagc aggaaauccu ugacaaccca     1980

guuaauacgg agaugcugga gaacauagug aaaaucuuga ccgucuucga agauaagagg     2040

augauuaaag agcagcucca gcaauuuuca gacgugcugg auggggucgu guuaaagaaa     2100

cuggaacguc ggcauuacac aggcugggga cgacuaagug cgaagcuccu gaugggcauc     2160

agagacaagc agucccaccu uacuauuuug gauuaucuga ugaaugacga cgggcucaac     2220

aggaaccuga ugcaguuaau caaugauagc aaccugucuu ucaaaucaau aauugagaag     2280

gagcagguua ccacagcuga caaagauauc caaaguauug uggccgaccu ugcaggaagc     2340

cccgccauca agaaagguau auugcagucc cucaagaucg uagaugaacu ggucucugug     2400

augggcuacc cuccacagac uauuguugug gagauggcuc gcgaaaauca gaccacaggg     2460

aaaggaaaga acaauucgcg gcccagauac aagagccuag agaaagccau caaggaauuu     2520

ggcucccaaa uucugaaaga gcauccgacc gacaaccagg agcucaggaa uaaccgccug     2580

uaucuuuacu auuugcagaa ugguaaggau auguacacug ggcaagaccu ggauauccac     2640

aacuuaucaa auuaugacau agaucacauu gucccucaga guuucaucac ggacaacucu     2700

aucgauaauc ucgugcugac aagcuccgca ggcaaccgag aaaaaggaga cgacguaccc     2760

ccacuugaga uugugcggaa gcguaagguc uuuugggaaa aacuguacca gggcaauuug     2820

augucaaaga gaaaauucga uuaucucacc aaggcugaga gagggggacu gacugaagcc     2880

gacaaagcgc gcuuuaucca ucggcagcua guugagacca gacaaauuac aaagaacgug     2940

gccaacauac ugcaccagag guucaauuac gagaaggaug accaugguaa caccaugaaa     3000

caggugcgaa ucgucacucu caagagcgcu cugguuucuc aguuucgcaa acaauuccag     3060

cuuuacaagg ugcgggaugu gaaugacuau caccacgcac augaugccua cuugaacggc     3120

guagucgcua auacacuguu aaaaguguau ccucagcucg aacccgaguu cguuuacggg     3180

gacuaucacc aguuugauug guucaaggcc aacaaggcaa cggcgaaaaa gcaauuuuac     3240

accaauauua ugcuguucuu ugcccagaaa gacagaauca uugaugaaaa cggagagauc     3300

cuuugggaca agaaauaucu ggacacugug aagaagguca uguccuacag gcagaugaau     3360

auagugaaaa agacagaaau ccaaaaaggc gaguucagua aggcuaccau uaaaccaaag     3420

gguaacagcu cuaaacuaau cccccguaag acaaauuggg auccuaugaa guacgggggc     3480

cucgacuccc caaacauggc cuaugcaguu gugauugagu acgcuaaagg aaagaauaaa     3540

cugguauuug aaaagaaaau cauacgcguc acuauuaugg agcggaaggc cuucgaaaag     3600

gaugagaaag cauuuuugga ggaacagggc uauagacagc cgaaggugcu ggccaaauua     3660

cccaaguaca cccucuauga gugugaagag gggaggcgac ggaugcuggc uucagcgaac     3720

gaagcccaga aaggaaauca acaggugcuu ccuaaccauc uggucacguu gcuccaccau     3780

gcagcuaacu gcgagguuag ugacggcaag agccuggauu acaucgaguc gaaucgcgaa     3840

auguucgccg agcuacuggc ccacgugucc gaauuugcua agagauauac ccuugcagag     3900

gccaaccuca auaaaaucaa ccagcuguuc gaacagaaua aggaggguga cauuaaagcg     3960

auagcucaau cuuucguaga uuugauggcc uuuaacgcaa ugggggcccc agcuagcuuc     4020

aaguuuuucg agacaacuau cgaaaggaaa cgguacaaua accugaagga guuacucaau     4080

ucaaccauua ucuaccagag uauuacagga cuguaugaau cccgaaagag acuugacgau     4140

aaacgccccg cagccacuaa gaaagcgggc caggcuaaga aaaagaaaua aggacuagua     4200

auaaggaaac uguaugaaug ucugugggca ggaagugaag agauccuucu guaaagguuu     4260

uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua auaaaucugg uagaaaaaug     4320

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa gaauu                                                      4395


<210>  3530
<211>  4428
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3530
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc aaagaaacca uacagcauug gacuggacau agguaccaac      180

uccguuggcu gggccguggu aacagaugac uauaaggugc cggcgaagaa aaugaagguc      240

cucgggaaua cugauaaauc ucauaucgag aagaaccuuc ugggagcuuu guuauucgac      300

ucaggcaaua cggccgaaga uaggcggcug aaacgcaccg cacgacguag auacacaagg      360

cggcgcaacc ggauucuaua ucuccaggag aucuuuagug aagagauggg uaagguggac      420

gauagcuucu uucacaggcu ggaagacucc uuccuuguua cugaggauaa gagaggggag      480

cgccauccca uuuuuggaaa ucuggaagag gaagugaaau accacgagaa cuucccuacc      540

aucuaucauu ugcgacaaua ccucgccgac aauccagaga aggucgaucu gagacuggug      600

uaucucgcuu uagcacacau aaucaaauuu aggggccacu uccugauuga aggcaaguuu      660

gacacacgga acaaugaugu acagcgucuu uuccaggagu uuuuggccgu uuacgacaac      720

accuucgaaa auucuucgcu gcaggagcaa aacgugcagg ucgaagagau ccuaacugac      780

aaaauuucaa agagcgcuaa aaaggaucgc gugcucaagc uguuucccaa ugagaaaagu      840

aacgggagau ucgccgaguu ccuuaagcug aucgugggaa aucaggcgga cuuuaaaaag      900

cauuucgagu uggaagagaa agcaccucuc caguuuucca aggauacaua cgaagaggag      960

cuggaagucu uacuggccca aauaggugac aacuaugcug aacucuuccu uucugccaag     1020

aaacuguacg auagcauuuu gcuauccggc auccugaccg uuacggacgu ggggacuaag     1080

gcaccacucu cagcuaguau gauucagagg uauaacgaac accagaugga ucuggcccag     1140

cuuaaacaau uuauccggca gaagcugagc gacaaauaca augagguauu cucugaugug     1200

uccaaggacg gauaugcggg cuacaucgau gguaagacca accaggaagc cuuuuauaaa     1260

uacuugaagg ggcuccugaa uaaaauugag ggaucaggcu acuucuuaga caagauagag     1320

cgagaagacu uucugcgcaa acaaagaaca uucgauaacg gcagcauccc ccaucagauu     1380

caccuacagg agaugagggc aaucauacgg cgccaggcug aauuuuaucc uuuccucgcc     1440

gacaaucaag auagaauuga gaagcugcuu acuuuuagga ucccauacua ugucgggccg     1500

uuggcucgug gaaagaguga cuucgccugg cugucucgga aauccgcaga uaagaucacc     1560

cccuggaacu ucgacgagau uguggauaaa gaaagcucgg ccgaggcuuu uaucaaucga     1620

augacaaacu acgaccucua ucugccuaau cagaagguuc uuccaaaaca cucucuguug     1680

uacgaaaagu ucaccgugua uaacgagcuc acgaaaguca aguacaagac ugaacagggu     1740

aaaacagcgu uuuucgaugc aaauaugaag caggagauuu uugacggcgu guucaaagua     1800

uacagaaagg uuaccaaaga uaagcugaug gacuuuuuag agaaggaguu cgaugaauuu     1860

aggauagugg accugacugg gcuagacaaa gaaaacaagg ucuucaaugc cuccuaugga     1920

acauaccaug aucucugcaa aauccuggac aaggauuuuc uugacaacag uaaaaaugag     1980

aagauuuugg aagauaucgu gcugacccuc acgcuguucg aggaccgcga gaugauccgg     2040

aagagauuag aaaacuauuc agaucugcuu accaaagagc aagucaagaa auuggaaagg     2100

cgccacuaca caggcugggg ucgacucagc gcugagcuga uucaugggau acggaacaag     2160

gaaucccgua aaacuauccu agacuaucug auugaugacg gcaauucuaa cagaaauuuu     2220

augcagcuca ucaacgauga cgcccugagc uucaaggagg agauugcaaa ggcccaggug     2280

aucggagaaa ccgauaaucu uaaccagguu gugucagaca uagcuggcag ucccgccauc     2340

aaaaagggga uuuugcaauc ccugaaaauc guagacgagu uagucaagau uaugggacac     2400

cagccugaaa auaucguggu ugagauggca agggagaacc aguucacaaa ucagggucgc     2460

cggaacagcc aacagagacu caaaggccug acugauucua uaaaggaauu ugggucacag     2520

auucuuaaag agcauccagu ggaaaauagu caacugcaga acgacagguu guuccucuac     2580

uaucugcaga auggacgaga uauguacacc ggcgaggaac uagacaucga uuaccugagc     2640

caguaugaca uugaucacau cauaccccaa gcguuuauca aggacaacuc cauugauaau     2700

cgcguccuca caucuucgaa ggagaaccgg gguaaaagcg acgaugugcc guccaaggac     2760

guagugagaa aaaugaaguc auacuggagu aaacugcuuu cugcuaaguu gaucacccag     2820

aggaaguucg acaaccugac uaaagccgag cgugggggcu uaacggauga cgauaaggca     2880

ggauuuauua aacgccagcu cguugaaaca cggcagauca ccaagcacgu cgccagaaua     2940

cuggacgaga gguucaauac ugaaaccgau gagaacaaua aaaagauucg acaagugaag     3000

aucgugacac uuaaaagcaa ccuggucucc aauuuucgga aggaguucga acuauauaaa     3060

guucgcgaga ucaacgacua ccaucacgcu caugaugccu aucucaaugc agugauuggc     3120

aaggcucugu ugggggugua cccucagcug gaacccgagu uuguauaugg agacuaccca     3180

cacuuccacg gucauaaaga aaacaaggcc accgcgaaga aauuuuucua cucaaauauc     3240

augaacuucu uuaagaaaga ugacgucaga acugauaaga auggcgagau uauauggaaa     3300

aaggacgaac acaucagcaa cauuaagaaa guguuaucuu auccucaggu uaauaucgug     3360

aagaaagucg aggagcagac agggggauuc uccaaggaaa guauucuccc caaaggcaac     3420

agcgauaagc ugaucccaag gaaaacgaag aaguuuuacu gggacaccaa aaaguauggu     3480

ggguucgacu cucccauagu ggccuacucc auccuuguua uugcugauau cgagaaaggc     3540

aagaguaaaa agcugaagac ugugaaagca uugguaggag ucacaauuau ggaaaagaug     3600

accuuugagc gggacccugu ggccuuccuc gagcgaaaag gcuauagaaa ugugcaagaa     3660

gagaacauca uaaagcugcc aaaauacuca cuauuuaagc uggaaaacgg gcgcaagagg     3720

cuucucgcua gcgcccguga gcugcagaaa ggaaaugaaa uugucuugcc gaaccaucug     3780

ggcacauuac ucuaucacgc aaagaauauc cauaaaguug augagcccaa gcaccuggac     3840

uacguggaua aacacaagga cgaguucaag gaacuucugg auguaguguc gaacuuuucc     3900

aaaaaguaca cuuuggcgga ggguaaucuc gagaaaauca aggaacugua ugcccagaac     3960

aauggggaag accuaaaaga gcuggcuucu agcuucauua accuucucac cuuuacggcc     4020

auaggagcuc cugcaaccuu caaguuuuuc gauaaaaaua ucgaccggaa gagauacaca     4080

ucaacuaccg agauucugaa cgccacauug auccaucaaa guauuacugg cuuauaugaa     4140

accaggaucg aucugaauaa gcucgguggc gacaaacgcc cagcagccac gaagaaagcu     4200

gggcaggcga agaaaaagaa guaaggacua guaauaagga aacuguauga augucugugg     4260

gcaggaagug aagagauccu ucuguaaagg uuuuuggaau uaugucugcu gaauaauaaa     4320

cuuuuuugaa auaauaaauc ugguagaaaa augagaucua aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  4428


<210>  3531
<211>  3561
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3531
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc acagacaaca aaccugagcu acauucucgg acuugaccug      180

gguauagccu ccguuggcug ggcgguggua gagaucaaug aaaacgagga uccaauuggg      240

uugaucgacg ugggagucag gauuuucgaa cgggcugagg ugccgaagac uggcgaaucu      300

uuagcccugu cacgccgacu agcacguagu acgagaaggc ucauccggcg cagagcccau      360

aggcugcuuc uggcuaaacg guuuuugaag cgcgagggua uacucagcac caucgaucug      420

gagaaggggc ugcccaauca agcaugggaa cuccgaguug ccggauuaga gagaaggcug      480

uccgcuauug aauggggcgc cgugcuuuug caccugauca aacaucgggg cuaucuaucu      540

aagcguaaaa acgagucgca gacaaauaac aaggagcucg gggcgcugcu uucaggaguc      600

gcacagaauc accagcuguu gcaaagcgac gauuaccgca cuccugccga acucgcucug      660

aaaaaguucg ccaaggagga aggucauauu agaaaccaga ggggcgcaua uacccacaca      720

uuuaaucggu uagaccugcu cgcugagcuu aaccuguugu ucgcccagca gcaccaauuu      780

gggaauccac auugcaaaga acacauccag caguacauga ccgagcuacu gauguggcag      840

aagcccgcgc ucaguggaga ggccauacug aaaaugcuug gcaaguguac ucaugagaaa      900

aacgaguuca aggcagcuaa acacacauau uccgccgaac gauuugugug gcugaccaag      960

uugaauaacc uccgcauucu ggaggauggu gcugaaagag ccuuaaauga ggaggaaagg     1020

caacugcuaa ucaaccaccc uuacgagaag ucuaaacuca cguaugcaca gguacggaag     1080

cugcuugggu ugagcgaaca ggccauuuuc aaacaucugc gcuacuccaa ggagaacgcu     1140

gaaucagcga cuuuuaugga gcucaaagca uggcacgcca ucagaaaggc ucuggagaau     1200

caaggacuua aggacaccug gcaggaucug gccaaaaagc cagacuugcu cgaugaaauc     1260

ggcacagcau ucagucugua caaaacugac gaggauauuc agcaguauuu aaccaacaag     1320

guucccaaua gcgugauaaa cgcccugcua gucucucuca auuuugacaa auucaucgaa     1380

cugucccuua agucauugag gaagauucug ccucucaugg agcaaggcaa acguuacgac     1440

caggcuugcc gggagaucua ugggcaucac uacggagaag ccaaccagaa gacaagccag     1500

cuguuaccag caauaccggc gcaagagauu cgaaaucccg uggugcugag aacccuuagu     1560

caggcuagga aagucaucaa cgccaucauu cgccaguaug guucuccugc acggguucau     1620

aucgaaacgg gcagagaguu ggggaagucc uuuaaagaaa ggcgcgagau ucagaagcaa     1680

caggaggaua aucgaacuaa gcgggaaagc gccgugcaga aauucaagga acucuucucg     1740

gacuuuucuu ccgaaccaaa aucaaaggau auacugaaau uccgucuaua cgagcagcaa     1800

cacggaaagu gucuguauag uggcaaagaa aucaacauuc acagacucaa ugagaagggu     1860

uacguagaga ucgaccaugc ucugcccuuu agcaggacau gggaugacuc cuucaacaau     1920

aaggugcuug ucuuggccuc ugaaaaccag aauaaaggga accagacccc uuacgagugg     1980

cugcaaggca agaucaacag cgaacgcugg aaaaauuuug uggcauuagu ucucggauca     2040

cagugcagug cugccaagaa acagcggcug cuuacucagg ugauugauga caacaaguuc     2100

auagauagaa aucugaacga cacaagguau aucgcgcgau uuuuguccaa uuacauucaa     2160

gagaaccucc ugcuagucgg caagaauaaa aagaacgugu ucaccccaaa ugggcagauc     2220

acggcccugc uccgcagccg guggggacug auuaaagcua gagaaaacaa uaacaggcac     2280

caugcacuug augccaucgu aguugcuugu gccacccccu cuaugcagca gaagauaaca     2340

cguuuuaucc gcuucaaaga ggugcacccg uauaagauug agaaucggua cgaaaugguc     2400

gaccaagagu caggugaaau cauuaguccu cacuuucccg agccaugggc auauuucaga     2460

caggagguga acaucagggu cuuugacaau cauccugaua cuguguugaa ggaaaugcug     2520

cccgaccgac cacaggcgaa ccaccaguuc guucaacccu uauucgugag ccgggccccu     2580

acccgcaaaa uguccggcca ggggcauaug gagacaauaa agucugcuaa aagacucgcc     2640

gaaggaauuu cgguacugag gaucccacuu acucagcuga agccgaaccu acucgagaau     2700

auggucaaca aagaacggga gcccgcacug uacgcuggcu ugaaggcccg acuggcagag     2760

uuuaaucaag auccugccaa ggcuuucgcg accccauuuu auaaacaggg ugggcaacag     2820

gugaaggcca uuagaguuga acaggugcag aaaagcggcg ucuuagugcg cgagaacaau     2880

ggaguagcag acaacgcuuc caucgugagg acagauguuu ucauaaagaa uaacaaauuu     2940

uuccucgucc ccaucuacac cuggcaagug gccaagggca uucugccuaa uaaagccauc     3000

guggcucaca agaacgaaga cgagugggaa gagauggaug agggggcaaa guuuaaauuc     3060

ucacuuuuuc caaaugaccu ggucgaauug aagacuaaaa aggaguacuu cuuuggauau     3120

uacauugguc ucgaucgugc cacgggcaac aucagucuga aagaacauga cggggagaua     3180

ucuaagggaa aggauggcgu uuaucgggug ggugugaaac uagcgcugag cuucgaaaag     3240

uaccagguag acgagcuugg gaaaaauaga cagauuugca ggccccagca gcgccagccg     3300

guccgaaaga gaccugcugc caccaaaaag gcaggccaag ccaagaaaaa gaaauaagga     3360

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     3420

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     3480

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaagaau u                                               3561


<210>  3532
<211>  3639
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3532
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc agccgcguuc aagccaaacc cgauuaauua cauacuggga      180

cucgacaucg guauugcuag cguuggcugg gccauggugg agaucgauga agacgagaac      240

cccauuugcc uuaucgaucu ggggguaagg guguuugaac gggcagaggu cccuaaaacc      300

ggagacuccu uggccauggc ucgccgauua gcacguucug ugagaaggcu gacacggcgc      360

agagcccaua ggcuacuccg ggcucgccga cugcuuaaga gagaaggcgu ucugcaggcc      420

gcggauuucg acgagaaugg uuugauaaag ucacucccaa acacucccug gcaacugagg      480

gcagccgcuc uggaucggaa acucacgccu uuagagugga gugccgugcu gcuucacuug      540

aucaagcauc guggguaucu gagccagcgc aaaaaugaag gagagaccgc agacaaggaa      600

cuaggcgcuc uccugaaagg cgucgccgau aacgcgcacg cccuucagac aggggacuuu      660

agaacuccag cagagcuggc uuugaauaag uucgagaagg aauccggaca uauuaggaac      720

cagcggggug auuacucuca caccuuuucg cgaaaagacc uccaagccga gcugaucuua      780

cuguucgaaa agcagaaaga guuuggcaau ccccacgugu cagggggacu caaggaaggc      840

auugagacac uucugaugac ccagcgcccu gcuuugagcg gugaugccgu acagaaaaug      900

cuagggcauu guacuuucga gccagcagaa ccgaaggccg cuaaaaacac auauaccgcg      960

gagagauuua ucuggcugac gaagcucaau aaccugagga uacuugaaca aggaagugag     1020

cggccccuga cugacaccga acgcgcaaca uugauggacg agccuuacag aaaguccaaa     1080

cucacuuaug cccaggcuag gaagcuguua ggccuggagg auaccgccuu cuuuaaaggc     1140

cuacguuacg ggaaggacaa ugcagaagcc ucuacacuca uggagaugaa ggcuuaucac     1200

gccauuagcc gggcacugga aaaggaggga cuuaaggaua aaaagucccc auugaaccug     1260

ucacccgaac uccaggacga gaucgguacc gcguucaguc uguuuaaaac ggaugaggac     1320

auuacuggcc gacuuaagga uagaauccag ccugaaaucc uggaggcuuu gcucaaacau     1380

auuagcuucg acaaguucgu ucaaauaucu cugaaggccu uaaggcgcau cgugccacug     1440

auggaacagg ggaaacggua cgaugaggca ugcgccgaga uuuacggaga ccacuauggc     1500

aagaaaaaua cagaagagaa gaucuaccua cccccgauac cugcugauga aauuagaaac     1560

cccgucgugc ucagggcccu gucccaggca cgcaaaguga ucaacggugu cguucgacgg     1620

uaugggucac cagcucguau ccacauugag accgccagag aagugggcaa gagcuuuaag     1680

gacaggaaag agaucgagaa gcgccaagaa gagaaucgga aagacagaga aaaggcggcc     1740

gcuaaauuca gggaguacuu uccuaacuuc guaggagaac ccaagaguaa agauauucuu     1800

aaguugcgac uguaugagca gcagcauggc aagugucucu acucugggaa agagauaaau     1860

cugggacgcu uaaacgaaaa ggguuaugug gagaucgacc acgcacugcc auuuucccgg     1920

acuugggaug acagcuucaa uaacaaaguc cuuguguugg gcucggaaaa ucagaacaag     1980

gggaaucaaa cacccuacga guacuuuaac ggaaaagaua auucuagaga auggcaggag     2040

uucaaggcca ggguugagac cucccguuuu ccucgcucaa agaaacagcg gauucuccug     2100

cagaaguucg acgaagaugg cuuuaaagag agaaaccuaa augacacgag guaugugaac     2160

cgauuccugu gccaauuugu cgcugaucgg augcgccuca ccgguaaggg gaaaaagaga     2220

guguucgcca guaauggcca gaucacaaac cugcuuaggg gauucugggg cuugcggaag     2280

guacgagcag aaaacgacag acaucacgcg cuggaugccg uuguggucgc uuguagcacu     2340

guggccaugc agcagaaaau cacccgcuuu gucagguaca aggagaugaa ugcauucgac     2400

gggaaaacaa uugauaagga gacuggagaa guguuacauc agaaaaccca cuuuccacag     2460

ccgugggagu ucuuugcuca ggaaguuaug auacgugugu ucgguaagcc cgacggcaag     2520

ccugaguuug aagaggccga cacaccagag aaacuccgga cccugcuugc agaaaagcug     2580

uccucuagac ccgaggccgu acacgaauau gucacuccuu uguucgugag cagggcucca     2640

aaccgcaaaa ugucagggca gggacauaug gagacgguua agagugcgaa acgacucgau     2700

gaaggcgugu ccguccugag agugccccua acacaacuga agcucaaaga ccuggagaag     2760

augguaaauc gggagaggga accgaaacuu uacgaggccu ugaaagcacg ccuggaagcu     2820

cacaaggaug acccugccaa agccuuugcu gagccauucu auaaguacga uaaagcaggu     2880

aaccguaccc aacaggugaa ggccguuaga gucgaacaag ugcagaagac uggggugugg     2940

guccggaauc auaacggcau cgcggacaau gcuaccaugg uuagggugga uguguuugag     3000

aaaggagaca aguauuacuu aguacccauu uacagcuggc aggucgccaa aggcauccuc     3060

ccugaucgcg cagugguuca ggggaaggac gaggaagauu ggcaacugau ugacgauucu     3120

uucaacuuua aauucucacu ucaccccaau gaccuggugg aggucaucac aaagaaggcc     3180

cgaauguucg gauauuuugc uaguugccac agaggcaccg guaacauaaa uauccggauu     3240

caugaccuag aucacaaaau cgggaagaac ggcauucucg aaggaaucgg ugugaaaacu     3300

gcacugagcu uccagaagua ccagauagac gaguugggca aagagauuag gccaugucgc     3360

cugaagaaga gaccucccgu uaggaaacgg ccagccgcga caaagaaagc ugggcaggcc     3420

aagaaaaaga aguaaggacu aguaauaagg aaacuguaug aaugucugug ggcaggaagu     3480

gaagagaucc uucuguaaag guuuuuggaa uuaugucugc ugaauaauaa acuuuuuuga     3540

aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            3639


<210>  3533
<211>  3645
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3533
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggcaccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc aaaguaccac guuggaauug acguggguac cuucagcgua      180

ggccuggccg cgauagaggu ggaugacgcu gggaugccaa ucaaaacacu cucccuuguc      240

ucucacauuc augauucagg acuggacccg gaugaaauca agagugccgu gacuagguug      300

gcaagcuccg gcauugcccg gcgcacgcga cguuuauaua gaaggaagcg gcgcagacug      360

cagcaacuag acaaauuuau ccagaggcag gguuggcccg uuauagagcu cgaagauuac      420

ucugacccuc uguauccaug gaaggugcgg gcugagcuug cagccucgua caucgcugau      480

gaaaaagagc gcggggagaa gcugucaguc gccuugcgac acauugcgag acauagggga      540

uggcggaacc ccuaugcaaa agugagcagu cucuaccugc cugacggccc auccgaugcc      600

uucaaggcua uccgugaaga gauuaagcgc gccucuggcc agcccguacc ugaaaccgca      660

acaguugggc aaauggugac ucugugcgag cucggaaccu uaaaacugag aggugagggc      720

gggguccuua gcgcuagguu gcagcagucc gacuaugccc gggaaaucca ggagauaugu      780

cgaaugcaag aaauuggaca ggagcuguac cgcaagauca uugauguggu guuugcggcc      840

gaaucaccaa aaggcagugc aagcucuaga gucgguaagg acccgcuaca gcccgggaaa      900

aauagggcuc ucaaggccuc cgacgcuuuc cagcgguacc gcaucgccgc acugaucgga      960

aaccuuagag uuagggugga uggcgagaaa cguauucugu caguagagga aaagaauuug     1020

guguuugacc accucgucaa ccugacaccu aagaaagagc cagaaugggu gaccauagcc     1080

gagaucuuag gcauugaucg ggggcaacug aucggaacug cuacaaugac cgacgauggu     1140

gaacgagcgg gcgcaagacc cccuacgcac gacacuaaua ggagcauagu uaacagucgc     1200

auugccccac ucguggauug guggaagacc gcuucugccc uugagcagca ugcaaugguc     1260

aaagcccugu ccaaugcuga gguggacgau uucgacagcc ccgaaggggc caagguacag     1320

gcauuuuucg cggauuugga cgacgauguu cacgcuaaac uagacucgcu gcaucucccg     1380

gugggacggg ccgcauauuc ugaggauaca cuggucagac uuacuaggcg caugcugucc     1440

gacggcgugg auuuguacac cgcccgacuc caagaauuug guaucgagcc uucauggaca     1500

cccccaaccc cucggaucgg ggaacccguc ggcaacccag cuguggaccg uguucugaag     1560

acggugagua gaugguuaga gagcgccacu aagacauggg gagcacccga gaggguaauu     1620

aucgaacacg uccgcgaggg cuucgugacc gaaaaacggg cuagagagau ggauggggac     1680

augaggcgac gcgccgcgcg gaaugccaag cuguuucagg agaugcagga aaaacuaaac     1740

guucagggaa agccuuccag agcugaucuc uggagguauc aaucugugca gcgucagaau     1800

ugccagugug cauacugcgg uagcccaauu acuuucucaa acagugagau ggaccacaua     1860

gucccgcgcg ccggccaagg guccacaaau acccgggaaa accugguggc uguaugucau     1920

agaugcaauc agagcaaagg aaacacgccc uuugccaucu gggcaaagaa caccucuauu     1980

gagggcgugu caguuaagga agcggucgag aggacacgac acugggugac ugauaccggu     2040

augcggagua cagacuucaa aaaguucacu aaagccgugg ucgagcgcuu ucagagagcu     2100

accauggaug aagagaucga cgccaggagc auggaauccg uugcauggau ggcuaaugag     2160

cuucggucuc gaguggccca gcauuucgca ucgcacggga caaccgugag aguauaucgc     2220

ggcagcuuga cugccgaagc uaggcgugcg uccggaaucu caggcaagcu gaaauuuuuc     2280

gacggggucg gaaagagucg gcucgauaga aggcaucacg ccauugacgc agcugugaua     2340

gccuuuacgu cugauuacgu ugccgagaca cuggcugugc gcagcaaccu uaagcaaucc     2400

caggcacacc gacaggaggc cccucagugg agagaguuca ccgguaaaga cgcggaacau     2460

cgggcugccu ggagggucug gugucaaaag auggaaaaac ugucagcauu gcucacugag     2520

gaucugcgcg acgaucgugu gguugugaug agcaauguaa gauuacggcu gggcaacggg     2580

ucugcccaca aggaaaccau cggaaaacua uccaagguca aacucaguag ccagcugucu     2640

guguccgaca uugauaaggc uucaagugag gcacuuuggu gcgccuugac aagggagcca     2700

ggcuuugacc ccaaggaagg ucugccugcg aauccagagc gccauauccg agugaacggg     2760

acccacgucu augcuggcga uaauauugga cucuuccccg uuagcgccgg cucgaucgca     2820

cugagagggg gauacgccga auuaggcucc ucuuuucacc augcucgggu guacaaaaua     2880

acuagcggua agaaaccggc cuucgcaaug cugaggguau auacaaucga ccuuuugccu     2940

uaccgcaacc aggaucucuu uucaguggag cugaagccac aaacgaugag uaugagacag     3000

gcugagaaaa agcuaaggga cgcccuggcg accgggaaug ccgaauaucu cggauggcug     3060

gucguggacg augagcuugu uguggacacu uccaagauug caacagauca ggucaaagcu     3120

guggaagccg aguugggcac cauccggcgu uggcgcguug acgguuucuu uucucccagc     3180

aagcugcgau uaagaccucu ccagaugucc aaagaaggca uuaagaaaga gucagcuccc     3240

gagcugagca agaucauaga uaggccaggg uggcuuccug cagugaacaa gcuguucagu     3300

gacggaaaug ucacaguagu gcggcgcgau ucuuugggca gaguuaggcu cgaauccacu     3360

gcccaucugc ccgugaccug gaaaguccaa aagcggccag ccgcuacgaa aaaggcgggg     3420

caggccaaaa agaagaaaua aggacuagua auaaggaaac uguaugaaug ucugugggca     3480

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     3540

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3645


<210>  3534
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3534
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc agccaggauu cuggcguucg acauaggaau cagcuccauu      180

gguugggcuu uuucugagaa cgaugaacuc aaggacugcg gcguucggau cuucaccaaa      240

guggagaauc caaagacagg ggaaucacuu gcccugccgc gccgauuggc acguagugcc      300

agaaagaggu uagcucggcg caaagcaaga cugaaccauc uaaagcaccu cauugccaau      360

gaguuuaaac ugaacuacga agauuaucag agcuucgacg agucccuugc uaaggccuac      420

aaaggaucuc ugaucucgcc cuaugaguug agguuucggg cgcucaauga acugcuguca      480

aagcaagauu ucgcacgcgu aauacuccau aucgccaagc gaagaggcua cgacgauauu      540

aaaaacagcg acgauaagga gaaaggugcu aucuuaaagg ccauuaaaca gaaugaagag      600

aagcuggcaa acuaucagag ugugggggag uaccuuuaua aagaauacuu ucagaaguuc      660

aaggaaaauu ccaaagaauu uacuaacguc aggaauaaga aagagucuua cgaacggugu      720

aucgcucaga gcuucuugaa ggacgagcug aaacuaauau uuaagaagca acgugaguuc      780

ggauuuuccu ucucuaaaaa guucgaagag gaagugcuca guguugccuu uuauaaacgc      840

gcgcugaagg auuucagcca ccuugugggc aacugcucau uuuucacgga cgagaaaaga      900

gccccuaaaa auuccccacu ggcauuuaug uucgucgcuu ugaccaggau uaucaaccuc      960

cugaauaacu uaaagaacac agaaggcauu cuguacacua aagaugaccu caaugcccuu     1020

cugaacgagg uguugaagaa ugggacccua acauauaaac agaccaagaa acugcucgga     1080

cugucagacg auuacgaguu uaagggugaa aagggcacuu auuucaucga guuuaaaaag     1140

uacaaagagu ucaucaaggc ucuuggggag cauaaccuga gccaggacga uuugaaugaa     1200

auugccaaag acauaacacu caucaaggau gagauuaaac ugaagaaggc auuagccaaa     1260

uaugaccuga accaaaauca gaucgauagu cuaucuaagc ucgaguuuaa agaccaccug     1320

aacauauccu ucaaggcucu uaaauuggua acuccccuga ugcucgaagg aaagaaguac     1380

gaugaggcgu guaaugaacu gaaccuuaaa guugcaauua augaggacaa gaaagauuuu     1440

cugccugccu ucaacgagac guacuauaag gacgaaguga cuaauccagu cguguugcgg     1500

gcuaucaaag aguaccgaaa ggugcucaac gcccuguuaa agaaauaugg caagguccac     1560

aaaaucaaua uugaacuggc acgcgagguu gguaagaacc auagccagag agccaaaauc     1620

gaaaaggagc agaacgagaa uuacaaggcu aaaaaggacg ccgaacuaga gugcgaaaaa     1680

cucgggcuga agauuaacuc gaaaaauaua cuuaaguuga ggcuguucaa agagcaaaag     1740

gaauuuugug cauauucugg agagaagauc aaaauuuccg aucuccagga cgagaagaug     1800

cuggaaaucg aucacaucua ccccuauuca cggaguuucg acgauagcua caugaacaaa     1860

guguuaguau uuaccaagca gaaucaagag aaacugaacc agacaccuuu cgaagcguuu     1920

ggcaaugacu ccgcuaagug gcagaagauu gaggugcuug ccaaaaacuu gccaacuaag     1980

aaacagaagc gcauacucga uaaaaauuac aaggacaagg aacaaaagaa cuucaaggau     2040

agaaaucuga acgacaccag guauaucgca cgucuagucc ugaauuacac aaaagauuau     2100

cucgacuuuc ugccgcuuuc ugaugacgag aacaccaagu ugaaugacac gcagaaaggc     2160

agcaaggugc auguugaggc caagucaggg augcugacca gugcuuuacg gcacacaugg     2220

ggauucuccg ccaaagaucg aaacaaccac cuccaucacg caauugacgc ugugaucauu     2280

gccuacgcga auaacagcau cgucaaggcc uuuucugauu ucaaaaagga acaggaguca     2340

aauagugcug aacuguaugc caaaaagaua agcgagcuug acuacaagaa caaaagaaag     2400

uuuuucgagc ccuuuuccgg uuucaggcag aaagugcugg auaagaucga cgaaauuuuc     2460

guaucuaaac cugagcgcaa gaaaccaucg ggcgcauugc augaagagac cuuucggaag     2520

gaagaggagu ucuaucaaag cuacggggga aaggaaggcg uucucaaagc ucuggagcua     2580

gguaagauca gaaaagugaa ugggaagauu gucaaaaacg gcgauauguu uaggguggac     2640

aucuucaagc acaagaaaac uaauaaguuu uacgccgucc ccauauauac aauggauuuc     2700

gcacugaaag ugcucccuaa caaggcgguu gcccgcucca aaaagggaga aauuaaggac     2760

uggauccuga uggaugagaa uuacgaauuu ugcuucucac uuuauaaaga caguuugauu     2820

cugauccaga ccaaggauau gcaggagcca gaguuugugu acuauaacgc uuucacgucu     2880

agcaccguau ccuuaauagu cucaaaacau gacaauaagu uugaaacacu cagcaaaaac     2940

cagaagaucc uguucaagaa ugccaacgag aaagaaguga uugcaaaguc uaucggcauu     3000

caaaaucuua aaguuuucga gaaguacauc guguccgcuc ugggggaagu cacuaaagcc     3060

gaguuucgac agcgggagga cuucaagaaa aagcgucccg cagccaccaa gaaagcugga     3120

caggcgaaga aaaagaaaua aggacuagua auaaggaaac uguaugaaug ucugugggca     3180

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     3240

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3300

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3345


<210>  3535
<211>  3756
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3535
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc aagcgaccug guucucggac uugauauugg uauaggcucc      180

guggggguag gaauccugaa caaggugacc ggcgagauua uccauaaaaa uucuaggauu      240

uucccagccg cgcaggcuga aaacaauuug guccggcgca caaaccgaca aggucguaga      300

uuagccaggc ggaagaagca ccgcagagug aggcugaauc ggcuauuuga ggaaucaggg      360

cucaucacug acuucacgaa aauaaguauc aaccugaauc cguaccagcu ucgcguuaag      420

ggacugaccg augaguugag caacgaagaa cucuuuauug cacugaaaaa uauggugaag      480

caucgaggca ucuccuaucu ggacgaugcc ucugacgaug gcaacucguc agucggggac      540

uacgcucaga uugugaaaga gaauagcaag cagcucgaaa caaagacucc cggacaaauc      600

caguuagaga gauaucagac cuacggucag cugaggggcg auuucacagu agaaaaagac      660

gggaagaaac accggcuuau aaacguuuuu ccuaccagug cauaucguuc cgaggccuug      720

cgcauucugc aaacucagca ggaguucaau ccacagauca cagaugaauu uauuaacaga      780

uaccuagaga uccucaccgg aaagaggaaa uauuaccaug gccccgguaa ugaaaagucu      840

cggacggacu acgggcgaua ucgcacuagc ggagagaccc uggauaacau cuucggcauu      900

cuuauaggca aaugcacauu uuacccugac gaguucagag cugccaaggc guccuauacu      960

gcacaagagu uuaaccuguu gaaugaccuc aacaaucuga ccgugccaac agagaccaag     1020

aaauuaucaa aggaacagaa aaaccagauc auuaauuacg ucaagaacga gaaagccaug     1080

gggcccgcua agcuguucaa guauaucgcc aaacuccuua guugugaugu ggcagacaua     1140

aagggauaca ggauugauaa aagcgguaag gcugaaaucc acacguuuga ggccuaucgg     1200

aaaaugaaga cucuggaaac auuggacauc gagcaaaugg aucgcgagac ccuagacaag     1260

cuggcguacg ugcucacucu gaauacagaa agagagggca uucaggaagc ccuugagcac     1320

gaguucgcag augggucuuu cucccagaaa caggucgacg agcugguuca auuuaggaag     1380

gcuaacucaa gcaucuucgg aaaaggcugg cauaauuuua gugugaaguu gaugauggag     1440

cucauuccug aacuguacga gaccucugaa gaacagauga cgauauuaac ccgucugggu     1500

aaacagaaga caacuuccag cucgaacaag accaaauaua ucgaugagaa gcuacucaca     1560

gaagagauuu acaauccagu aguggccaaa ucuguccggc aggcuaucaa gaucgugaac     1620

gccgcaauua aagaauaugg ggacuucgau aauauaguua ucgagauggc ccgagaaacu     1680

aacgaggacg acgagaagaa agcuauucaa aagauccaga aggcgaauaa agaugaaaag     1740

gacgcagcca ugcugaaagc ugccaaccag uacaacggca aggcagagcu uccgcacucc     1800

guguuucaug gacacaaaca guuggccacc aagauuagac uguggcacca acagggcgaa     1860

aggugccucu auacagggaa gaccaucuca auacaugauc ugaucaauaa caguaaucag     1920

uucgaggucg accacauucu uccccugagc aucacuuuug augacuccuu ggcuaacaaa     1980

gugcucguau acgccacggc aaaucaggaa aagggacaac gcacaccuua ucaggcgcug     2040

gauucuaugg acgaugcuug gagcuuccgg gaguuaaaag ccuuuguuag agagucaaag     2100

acccugagua acaaaaagaa ggaauaccua cucacugagg aagacauuuc caaauucgau     2160

gugaggaaga aauuuaucga gcgcaaucug gucgacaccc gauacgcaag ccgggugguc     2220

cuuaacgccu ugcaggaaca uuuccgugcu cacaagauag auacaaaagu gucuguugug     2280

agaggucaau uuaccucaca gcugaggcgc cauuggggca uugagaagac ucgggacaca     2340

uaucaccacc augccguaga cgcacucauc auugcugcca guagccagcu gaauuuaugg     2400

aagaaacaga agaacacgcu ggucuccuac ucugaggauc aacuuuugga caucgaaacc     2460

ggggagcuca uaucggauga cgaauauaaa gagagcgugu ucaaggcgcc auaccagcac     2520

uucguugaua cucugaaauc caaggaguuu gaagacucaa uccuauucag uuaucaggug     2580

gauucuaaau uuaauagaaa gauuagcgac gccacaaucu acgcuaccag gcaggcaaag     2640

gucggaaaag auaaggccga cgagacauau gugcugggca aaauuaagga uaucuacacu     2700

caagacgguu acgaugcuuu caugaaaaua uauaagaagg acaaauccaa guuucucaug     2760

uaccgacaug acccccagac cuucgaaaaa guaauugagc cuauccugga aaacuaucca     2820

aauaagcaga ucaacgagaa agggaaggag gugcccugua auccguuucu uaaguacaaa     2880

gaggaacacg gcuauauucg caaguacagc aaaaagggaa acggcccuga aaucaaauca     2940

uugaaguauu acgauucuaa gcuggggaac cacauugaca uaacgcccaa agauuccaau     3000

aacaagguug ucuuacagag ugugagccca uggcgggccg acguguacuu caauaaaacc     3060

acaggaaagu augagauccu cggucugaaa uacgcagauc uucaauuuga aaagggcacu     3120

gggaccuaua agauuucuca ggagaaauac aacgacauca agaaaaagga gggagucgau     3180

uccgacucag aguucaaauu uacacuguau aagaaugauu ugcuccuggu uaaagacacu     3240

gaaaccaagg aacagcaacu auucagauuu cugaguagga cgaugccuaa gcagaaacau     3300

uacguggagc ucaagcccua ugauaaacag aaguucgaag gcggugaggc gcugauuaaa     3360

gugcuuggga acguagccaa uagcggccag ugcaagaagg gauugggcaa aucgaacauc     3420

uccauauaca agguccguac agacgugcug gggaaucaac acaucauuaa aaacgaggga     3480

gacaagccaa aauuagauuu caagcgcccc gcugccacca agaaagcagg ccaggcuaag     3540

aaaaagaaau aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     3600

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     3660

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3756


<210>  3536
<211>  4314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3536
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc aacccaguuc gagggauuua caaaccugua ccaaguuagc      180

aagacucuca gguucgaacu uauuccacag gguaaaacgc ugaagcauau acaggagcag      240

ggcuuuaucg aagaggacaa ggcccggaau gaucacuaua aagaauugaa gccgauuauc      300

gaccgcauuu acaaaaccua ugcggaucaa ugcuuacagc uggugcagcu agacugggag      360

aaccucuccg cugccaucga uucuuaccga aaggagaaaa cagaagagac ucguaaugca      420

cugauagagg aacaggccac cuauagaaac gcuauccaug acuacuucau ugggaggaca      480

gauaaucuua ccgacgcaau caacaagcgg cacgccgaga uuuauaaggg acuguuuaaa      540

gcugaauugu ucaauggcaa gguacucaaa caacugggua cugugacaac cacggagcau      600

gaaaacgccc ugcuccgcuc auuugauaag uucacuaccu acuuuagugg guucuacgag      660

aauaggaaaa acgucuuuag cgcggaagac aucuccacag caauacccca cagaauugug      720

caggauaauu ucccuaaguu uaaagagaac ugucacaucu ucacucgguu aauuaccgcc      780

guuccaucuc ugcgcgagca uuucgaaaau gugaagaagg cuaucggaau cuuugucucg      840

acaucaauug aggaaguguu cagcuuuccc uucuauaacc agcuuuugac ccagacgcaa      900

auagaccugu acaaucagcu acucggcggc aucagucgag aggccgggac ugagaaaauu      960

aagggacuga acgaaguacu uaaccuggca auccagaaaa augaugagac agcucacaua     1020

auugccuccu ugccucauag auuuauccca cucuucaagc aaauccuguc ugacaggaac     1080

acccuuagcu uuauucugga agaguucaaa uccgacgaag agguuaucca gucauuuugc     1140

aaguauaaga cucuccuucg gaaugaaaac gugcuggaga cagcggaggc cuuguucaau     1200

gaacuaaaca guauugaucu gacccacaua uuuaucagcc acaaaaagcu cgagacgauu     1260

ucuuccgcac ugugugacca uugggauacc cuucguaaug cucuguacga acgcagaauc     1320

ucagaguuga cagguaaaau cacuaagagc gccaaagaga agguccagag gagucucaag     1380

cacgaagaca uuaaccugca ggagauaauc ucugcugccg gcaaagaauu auccgaggca     1440

uucaagcaga aaaccagcga aauucugucg caugcccacg cugcgcuaga ucaaccccuc     1500

ccuacaacuc ugaagaaaca ggaggagaag gaaauccuua agucucaguu ggacucccug     1560

cucgggcugu aucaucuucu ggauugguuu gcaguggacg agucaaauga aguggaucca     1620

gaguucagug cccgguugac cggaauuaaa cucgaaaugg agccgagccu guccuucuac     1680

aacaaggcuc gaaauuaugc cacuaaaaag cccuacucug ucgagaaauu uaaguuaaac     1740

uuccaaaugc cuacccuggc aagcggcugg gacguuaaua aagaaaagaa caauggugcc     1800

auccuauuug ugaagaacgg gcucuacuau cugggaauaa ugccaaaaca gaagggccgc     1860

uacaaagcuc uuucauucga gcccacugaa aagacgagug agggcuuuga uaaaauguau     1920

uacgacuauu ucccugacgc cgcaaagaug aucccaaagu gcuccacaca guugaaagcg     1980

guaaccgcuc acuuucagac ucacaccaca cccauucugc ucagcaacaa uuucaucgaa     2040

ccgcuggaga uuaccaagga gaucuacgau uuaaacaauc cugaaaaaga gcccaagaaa     2100

uuucaaacug ccuaugcaaa gaagacaggg gaccagaaag gauacagaga agcccugugu     2160

aaguggauag auuucacgag ggacuuucuu ucuaaauaca ccaagacuac aucaauugau     2220

uugaguagcc uccggccauc cucucaguau aaagaccugg gugaguacua ugcugagcua     2280

aacccucugc ucuaccauau cucguuccag cgcauugccg aaaaggagau cauggaugca     2340

guggaaaccg gcaagcugua ucuuuuucaa auauacaaua aagacuucgc uaaggggcac     2400

cauggaaaac ccaacuugca cacacuguau uggacuggcu uauucagccc agagaaucuc     2460

gccaagaccu ccaucaaacu gaacggucag gcggaauuau uuuacagacc caagucaagg     2520

augaaacgua uggcccaccg gcugggggag aagauguuga auaagaaacu caaggaucag     2580

aaaacgccua uuccagacac ccuguaccaa gagcuauaug auuacgucaa ccaucgacug     2640

agucacgacc ucucugauga agcuagagca cugcuuccga augugaucac aaaggagguu     2700

agccaugaaa uuaucaaaga caggcgcuuc acuuccgaca aguuuuucuu ucacgugccc     2760

auaaccuuga acuaucaggc cgcuaauuca ccuagcaagu ucaaccagcg ggucaaugcc     2820

uaccugaaag agcauccaga aacacccauu aucggcaucg auagaggaga gaggaacuua     2880

auuuauauca cugugauuga cucuaccggc aagauacucg agcagcgcuc ccugaacacg     2940

auccaacagu uugauuacca gaaaaagcug gacaaucgag aaaaagagcg gguagcagcg     3000

cgucaggccu ggaguguugu ggggacaauu aaggaucuua agcaaggaua ucuaagccag     3060

gucauccacg aaauugugga ccucaugauc cacuaccagg cugucguggu ucuggagaac     3120

uugaauuucg guuuuaaauc uaagagaacc ggcauagccg aaaaagcagu guaccagcaa     3180

uucgagaaga ugcugaucga uaaauuaaac ugccucguac ugaaggacua uccugcugag     3240

aaggucgggg gagugcuuaa uccauaccag cugacugauc aguuuacauc cuucgccaaa     3300

augggcaccc aaucagguuu cuuguuuuau guucccgcac cguacacaag uaagauugac     3360

ccucucaccg gguucgugga uccauuuguc uggaaaacaa ucaagaacca ugaaagcagg     3420

aaacacuucc uggagggcuu ugacuuccua cauuaugaug ugaagacggg agacuuuauu     3480

cugcacuuca agaugaaucg caaccuuucg uuucagcggg gccuccccgg guucaugccu     3540

gccugggaca ucguauuuga gaaaaaugaa acccaguucg augcuaaggg aacucccuuu     3600

auagcgggua aaagaauugu gccaguuauc gagaaccaua gguucacagg ccgauaccgc     3660

gaccuguauc cugccaauga auugaucgca cuguuagagg aaaaggggau ugucuuccgg     3720

gauggaucca acauacuccc caaacugcuu gagaaugacg auucucacgc uaucgacacc     3780

augguggccc ugauuagaag cguguugcag augaggaacu caaaugccgc uacaggcgaa     3840

gauuacauca acaguccagu ccgugaccuc aacgguguuu guuuugauuc ccgcuuccaa     3900

aauccggagu ggccuaugga cgcagaugcc aacggggcgu accacauugc ucugaagggc     3960

cagcuacugc uuaaucaucu caaagagucu aaggaccuga aguugcagaa cggaaucagc     4020

aaucaggacu gguuagccua uauacaagaa cugcggaaca aaagacccgc agccacuaag     4080

aaagcuggcc aggcaaagaa aaagaaguaa ggacuaguaa uaaggaaacu guaugaaugu     4140

cugugggcag gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau     4200

aauaaacuuu uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           4314


<210>  3537
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3537
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc aagcauuuac caggaguucg uuaacaagua uucccugucu      180

aaaacccuca gguuugaacu uauaccacaa ggaaagacac uggagaauau caaggcccgg      240

gguuugauuu uagacgauga aaaacgcgcg aaggacuaca aaaaggcuaa acagaucauu      300

gauaaguauc aucaguucuu uaucgaggaa auacugucaa gugugugcau cagcgaggac      360

cuacuccaga acuacuccga uguauauuuc aagcugaaaa agucugacga ugacaaucuu      420

caaaaagauu uuaagucggc caaagacacu auuaagaaac agaucucaga guacauuaag      480

gauagcgaaa aguucaaaaa ccuguuuaau cagaacuuga ucgacgcaaa gaaaggccag      540

gagagugauc ucauacugug gcugaagcaa uccaaagaca augggauuga acucuucaag      600

gccaacucug acaucacgga uauugacgag gcuuuagaga ucaucaagag cuuuaaagga      660

uggaccacau auuucaaggg cuuucacgaa aaucguaaaa acguguacuc cucaaaugau      720

auuccgacaa guauaaucua ccgaauuguc gacgauaacc ugcccaaguu ccuugagaau      780

aaagcaaagu augagagcuu gaaggacaaa gccccugagg cuaucaacua cgaacagaua      840

aagaaagauc uggccgagga gcuaaccuuu gacauugauu auaagacauc ugaagugaau      900

cagagaguuu ucucccucga cgagguguuc gaaaucgcga acuuuaacaa uuaccugaac      960

cagucaggua ucaccaaauu caauacuauu aucgggggaa aguuugucaa cggcgagaau     1020

acaaagagga aaggcauuaa cgaauauaua aaucuuuaca gccaacagau caacgauaag     1080

acccugaaaa aguauaaaau gaguguguug uucaagcaga uucucucuga cacggagucc     1140

aagagcuuug uaaucgacaa acuggaggau gacucggaug uugugacuac caugcaaucu     1200

uucuacgaac agaucgcagc cuuuaagaca gucgaggaaa aauccauuaa ggagacuuua     1260

ucacuccugu ucgacgaucu uaaagcucag aagcuggacu ugaguaaaau auacuuuaag     1320

aaugauaaga gccuaaccga ccugucccag caaguguucg augacuauuc ugugaucggg     1380

acagccgucc ucgaauacau uacccagcag aucgcaccca aaaaccugga uaauccaagc     1440

aagaaagagc aggagcuuau ugcuaagaaa acggaaaagg ccaaguaucu gucauuggag     1500

acuaucaaac ucgcgcugga agaguuuaac aagcaucggg acauagauaa acaaugucgc     1560

uucgaggaaa ucuuagccaa uuuugcagcu auuccuauga ucuucgacga gauugcccag     1620

aacaaggaca aucuggcuca gaucaguaua aaauaccaga accaaggaaa gaaggaucua     1680

cuccaggccu ccgcagaaga cgauguuaaa gccauuaagg accugcuuga ucagacaaac     1740

aauuugcugc acaaacucaa gaucuuccau auuagccagu cugaggacaa agcuaacauc     1800

cuggauaagg acgaacacuu uuaucuugug uucgaggagu gcuacuuuga acuggcgaau     1860

auaguaccau uguauaacaa aaucagaaau uacauuaccc aaaagcccua cucagaugag     1920

aaguucaaac ucaacuuuga aaauaguacu cuggcaaacg guugggacaa gaauaaagag     1980

ccugauaaca cagccaucuu auucauuaag gacgauaaau auuaccuggg cgugaugaau     2040

aagaagaaca auaaaaucuu ugacgacaag gcuauaaaag aaaacaaggg ggagggauau     2100

aaaaagauug ucuacaagcu acucccaggc gccaauaaaa ugcugccgaa gguguucuuu     2160

agcgcaaaau ccaucaaguu cuauaacccc ucugaggaua uccuuaggau ucggaaccac     2220

ucgacccaua cgaaaaaugg uagcccucag aagggguacg aaaaguuuga guucaacauc     2280

gaagacuguc gcaaauuuau ugauuucuau aagcagucca uaucaaaaca cccagagugg     2340

aaggacuucg gauuucgauu cagugauacc caaagauaca auucuaucga cgaauuuuac     2400

agggagguug agaaccaggg cuauaaauug acauucgaaa auauuagcga guccuacauc     2460

gauucagugg ucaaccaggg caagcuguau cucuuucaga uuuacaauaa ggacuucagc     2520

gccuauucua aagggcggcc caaccugcau acuuuauacu ggaaggcucu guuugaugaa     2580

cguaaucuuc aagacguggu auauaaauug aacggagagg ccgaacucuu cuaccgcaag     2640

caguccaucc cuaaaaagau aacccaccca gcaaaagaag cgaucgcuaa uaagaacaag     2700

gauaauccca aaaaggagag uguuuuugaa uacgaccuga uuaaagacaa gagauucaca     2760

gaggauaaau uuuucuucca cugcccgauc acuauuaacu uuaagagcuc uggugccaau     2820

aaguucaacg acgaaaucaa ccuacugcuc aaagagaagg caaaugaugu gcauauacug     2880

uccauugaca ggggcgagcg gcaccuugcc uauuacaccu uggucgaugg gaaaggaaac     2940

aucaucaagc aggacacauu uaauauuaua ggcaacgauc gaaugaaaac caauuaucau     3000

gacaagcugg cugccaucga aaaggaucgc gacucagcaa gaaaagauug gaagaaaauu     3060

aacaauauca aggagaugaa agaagguuac uuaagucagg ugguccacga gauugcuaag     3120

cucgugaucg aauauaacgc cauaguugug uucgaggacc ugaauuuugg guucaagagg     3180

ggccgguuua aaguagagaa gcaagucuac cagaaacuug aaaagaugcu gaucgagaaa     3240

uugaacuauc ucguguucaa ggauaaugaa uuugacaaaa cuggaggcgu ucugcgcgcg     3300

uaccagcuaa cggccccuuu cgagacauuu aagaagaugg ggaaacagac cggaauuauc     3360

uacuaugugc ccgcugguuu cacuagcaag auuuguccag ucaccggcuu ugugaaccaa     3420

cuguacccua aauaugaauc gguauccaag ucucaggagu ucuucagcaa auuugacaag     3480

aucugcuaca aucucgauaa gggguauuuc gaguuuucau ucgacuacaa aaacuuugga     3540

gauaaggcag ccaaaggcaa guggacaaua gcuaguuucg guuccagacu gauuaauuuu     3600

aggaacucug acaaaaauca uaacugggau acccgugaag uguaucccac uaaggagcuu     3660

gaaaaguugc ugaaagacua cagcaucgag uacgggcacg gcgaguguau caaggccgca     3720

auuugcggag aauccgauaa aaaguucuuu gcgaaauuaa caucaguucu caacacgauc     3780

cugcagaugc ggaauagcaa gaccggcacu gagcuugacu aucugauaag uccagucgcc     3840

gaugugaacg ggaauuucuu ugacucucga caagcuccca agaacaugcc ucaggaugcc     3900

gacgcaaaug gagcuuacca cauuggucua aaaggccuca ugcuguuggg gagaaucaag     3960

aacaaucagg aaggcaaaaa gcugaacuua gugauuaaaa augaggaaua uuucgaguuu     4020

guccagaaca ggaauaacaa gcgcccagcc gcaacuaaaa aggccggaca agcuaagaaa     4080

aagaaauaag gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag     4140

auccuucugu aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau     4200

aaaucuggua gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  4293


<210>  3538
<211>  4439
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3538
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcagac aagaaauaca gcauuggacu ggauauaggu      180

acaaacuccg uuggcugggc cgugaucaca gacgaguaua agguaccauc uaagaaauuc      240

aaggugcucg ggaauacuga uaggcauuca auuaaaaaga accuuaucgg agcgcuguug      300

uuugacagug gcgaaacggc ugaggccacc cgguuaaaac gcacagcacg acguagauac      360

acuaggcgga agaaucgcau uugcuaucug caggaaaucu ucagcaacga gauggccaag      420

gucgaugacu ccuuuuucca cagacuagaa gagucuuuuc ucguggagga agauaaaaag      480

caugagaggc acccgauauu cgguaauauc guugacgaag uggcuuacca ugagaaauau      540

cccaccauuu accaucugcg gaagaaacuu gucgauucga cagacaaggc agaucugcgc      600

uugaucuauc ucgcccuggc ucacaugauu aaauuucgag ggcacuuccu gaucgaggga      660

gaccucaacc cugauaauuc agacguggac aaguuauuua uacaacuggu acagaccuac      720

aaccagcuuu ucgaagagaa uccaauuaac gccagcggcg uugaugcgaa ggcaaucuug      780

agugccagac uguccaaauc uaggcggcua gaaaaucuca uugcucagcu gcccggcgag      840

aagaaaaacg ggcuuuuugg aaaucugauc gccuugagcc ucggucugac uccuaacuuc      900

aaguccaauu uugacuuagc agaagaugcu aaacugcaac ucucaaagga cacauacgau      960

gacgaucuug acaaccuguu ggcccagauc ggcgaucagu augcggaccu auuccuggcc     1020

gcaaagaacc ucagugaugc uauucugcuu agcgacauac ugcgugugaa uaccgagauc     1080

acgaaagccc cauugucugc uuccaugauu aagcgcuacg acgagcacca ucaggaucuc     1140

acucuguuaa aagcccuggu cagacaacag cuacccgaaa aguauaaaga gaucuucuuu     1200

gaccagucaa agaacgggua cgcaggauau auagauggcg gugccagcca ggaagaguuc     1260

uacaaguuua uuaaaccuau ccucgaaaag auggacggga ccgaggagcu gcuugugaaa     1320

uugaauaggg aagaucugcu ccggaagcaa cgaacauucg acaacggaag uaucccacac     1380

cagauucacc ugggcgagcu ucaugcuauc cugcgcagac aggaagauuu uuauccguuc     1440

uugaaagaca auagggagaa gauugaaaag auacucacuu uucggauccc cuacuacgug     1500

ggcccucugg cgcgcgggaa cucuagauuc gcauggauga ccaggaaauc cgaggagaca     1560

auuaccccau ggaauuuuga agaggucguu gauaagggag ccagcgcuca aucguucauc     1620

gaacguauga cgaacuuuga caaaaauuua cccaacgaga aggugcugcc uaaacacucu     1680

cuacucuaug aguacuucac uguauauaau gaacugacaa aggugaaaua cgucaccgag     1740

gguaugcgga agccagccuu ucuuuccggc gaacagaaga aagcaaucgu ggauuugcug     1800

uucaagacua accgaaaagu uacagugaag cagcucaaag aggacuauuu caagaagauu     1860

gaauguuuug auucagucga gauaaguggg guggaggaca gauucaaugc cagccuggga     1920

accuaccaug acuuacugaa aaucauuaag gauaaagacu uucuugauaa cgaagagaau     1980

gaagacaucu uggaggauau uguacucacg cugacccuau ucgaagacag ggagaugauc     2040

gaggaacgcc ugaagacaua ugcucaccuc uuugaugaca aaguuaugaa gcagcugaag     2100

cggagaaggu acacuggcug gggucgccuu ucccgaaaau ugauaaacgg gauccgggau     2160

aagcaaucug gcaaaaccau ucuggacuuc uuaaagagcg auggauuugc caaccguaau     2220

uucaugcagc ucauccauga cgauucacug acauuuaaag aggacauuca gaaggcacag     2280

gugaguggcc aaggggacuc ccuucacgaa cacaucgcga accuggcugg aagccccgcc     2340

auaaagaaag guauuuugca gacugucaag guggucgaug agcucgugaa aguuaugggc     2400

agacauaagc cggaaaauau cgugauugag auggcaaggg agaaccagac cacacagaaa     2460

gggcaaaaga acucucgcga acggaugaaa agaaucgagg aaggaauaaa ggagcugggc     2520

ucacagaucc uaaaggagca cccuguagaa aauacccagc ugcagaauga gaaacucuac     2580

cuguauuacc uucaaaacgg uagggacaug uaugucgauc aggaauugga cauuaaucga     2640

cugagugauu acgacgugga ucauaucguu ccccagagcu ucuuaaagga cgauuccauu     2700

gacaacaaag ugcucacucg cucugauaag aaucggggga aaucggacaa cgucccaagc     2760

gaggaagugg uaaagaagau gaaaaauuau uggagacaac ugcuuaacgc caagcugauc     2820

acgcagagga aauuugacaa ucuaacaaag gcugagcgug gcggacucuc cgagcuggau     2880

aaagccggcu ucauaaagcg ccaguuggug gaaacccggc agauuacuaa gcacguugca     2940

caaauccugg acucaagaau gaacaccaaa uacgaugaga acgacaaguu aaucagggaa     3000

gucaaaguga uuacacucaa gaguaaacug gugucugauu uucgaaagga cuuccaguuc     3060

uauaaggucc gggagaucaa uaacuaccac caugcucacg augccuaccu uaaugcgguu     3120

guggggaccg cccugauuaa aaaguauccu aaauuggaaa gcgaguuugu guacggagac     3180

uauaagguau acgauguccg caaaaugaua gcuaaguccg agcaggaaau cgguaaggca     3240

acugccaaau auuucuuuua cucaaacauu augaauuucu uuaagacaga gaucacgcuc     3300

gcuaacggcg aaauuagaaa aaggccccug aucgagacca auggggaaac uggagagaua     3360

gugugggaca agggccggga uuucgccaca guucgaaaag ugcuaagcau gccacagguc     3420

aacaucguga agaaaaccga gguucaaaca gguggguuuu cuaaggaauc cauucugccc     3480

aagagaaaua gugacaaacu uaucgcacgc aagaaagauu gggacccuaa gaaauauggc     3540

ggauucgaca gcccaacugu ggcguacucu guacucgucg uggccaaggu ggagaagggc     3600

aaauccaaga aacugaaguc agucaaagaa uugcugggga uuaccaucau ggagaggagu     3660

agcuuugaga agaacccgau agauuucuua gaagcuaagg gauacaaaga gguuaagaaa     3720

gaccucauua ucaagcugcc caaauauucg cuuuuugaac uggagaaugg ccguaagcgg     3780

auguuggccu ccgcugguga acuccagaag gggaacgagc uggcacuacc uucuaaauac     3840

gugaauuucc uguaucuugc cagccauuac gagaagcuca aaggaucacc agaagauaac     3900

gagcagaagc agcuguucgu agaacaacac aaacauuauu uggacgagau cauugaacag     3960

auaagugagu uuuccaagag agugaucuua gcagaugcca aucuggacaa aguccucucu     4020

gcuuacaaca agcacaggga uaagcccauu cgcgagcagg cggaaaacau cauucaccug     4080

uucacgcuua ccaaucuggg cgccccugca gcuuuuaaau auuucgacac aacuaucgau     4140

cgaaagagau acaccagcac aaaagaggug uuggacgcca cucuaauaca ucaauccauc     4200

accggucugu acgaaacgcg gauugaucuc ucacagcugg gcggggacaa gaggccagcc     4260

gcuaccaaaa aggcaggaca ggccaagaaa aagaaauaag gacuaguccc uguucccaga     4320

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      4439


<210>  3539
<211>  5222
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3539
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaac uucaagauuc ugccaauagc caucgaccuc      180

ggaguuaaaa auaccggugu guuuagcgcg uucuaccaga agggcacauc ccuugagagg      240

cuggauaaca agaaugggaa aguauaugaa uugucuaagg acucauacac uuuacugaug      300

aacaaucgga cggcucgccg acaucaacgu agaggaauug auaggaaaca gcuagugaag      360

cggcucuuua aacugaucug gaccgagcag cuuaaccugg aaugggacaa ggauacacag      420

caagccauua guuucuuguu uaaucgcaga ggcuucagcu uuaucacuga cgguuauucc      480

ccggaguacc ucaacauagu ccccgaacag gugaaggcaa uccugaugga uauuuucgac      540

gauuauaaug gggaggacga ucuggacucu uaccucaaau uagccaccga gcaggaaucg      600

aagaucucag agauuuauaa caaacugaug cagaagaucc uugaauuuaa auugaugaag      660

cugugcacag auauaaaaga cgacaagguu agcaccaaga cucuaaaaga gauuacaagu      720

uacgaguucg aacuccuggc ugauuaccuu gcaaauuauu ccgagucucu gaagacccaa      780

aaauuuagcu acacggacaa gcagggaaac uugaaagaac ucuccuauua ccaccaugau      840

aaguauaaua uccaggaguu ccugaagagg cacgccacua uuaacgaccg gaucuuagau      900

acccugcuca cagacgaucu ugacaucugg aauuuuaacu ucgaaaaauu cgauuuugac      960

aagaacgagg agaaacugca gaaucaagaa gauaaggacc auauucaggc ucacuugcac     1020

cauuucgugu uugccgucaa caaaauaaag ucagagaugg cgaguggcgg ccgccaccga     1080

agccaguacu uccaagaaau cacuaaugug cuagacgaga acaaucauca ggaaggguau     1140

cugaagaacu uuugugagaa ucuccacaac aaaaaguacu cuaaucuguc cguuaaaaac     1200

cuuguaaauc ugauuggaaa cuugucaaau cucgagcuga agccuuuaag aaaauacuuc     1260

aacgauaaga uccacgcaaa ggccgaccau ugggaugaac agaaauuuac cgagacauau     1320

ugccacugga uacuggguga auggagggug ggcgucaagg accaggauaa aaaggacggg     1380

gcuaaauaca gcuauaagga ucuauguaau gagcucaaac aaaaggugac caaggccgga     1440

cugguggacu uccuuuugga acuggaucca ugccggacga uucccccuua ccucgacaac     1500

aacaaucgua aaccacccaa gugucagagu cugauccuua acccuaaauu ucuggauaau     1560

caguauccaa acuggcagca auacuugcag gagcucaaga aacugcaguc uauccagaau     1620

uauuuagacu ccuucgagac ugaucugaag guccuaaaga gcucgaaaga ccaaccguac     1680

uuuguugaau acaagucuuc caaccagcag auugcaucag gccagcgcga cuauaaagau     1740

cucgacgcua gaauccugca auucauuuuu gauaggguga aggccaguga cgagcuuuug     1800

cugaaugaaa uauacuucca ggcgaaaaag cucaagcaga aagccagcuc cgagcuggag     1860

aaguuagaau cuagcaaaaa gcuggaugag guaaucgcaa acucacaacu uagucagauu     1920

uugaaauccc agcauacaaa ugguaucuuc gaacagggga ccuuucucca ccuggugugc     1980

aaguauuaca agcaacggca gcgagcucgc gacagcagac uauauaucau gcccgaguac     2040

agguaugaua aaaagcugca uaaauacaac aauacuggac gguucgacga ugacaaccag     2100

cuccugacau acuguaauca caagccucgc cagaaaagau aucaacuuuu gaacgaucug     2160

gccggcgucu uacagguguc uccaaauuuu cucaaggaca aaauuggcuc agaugacgac     2220

cuguucauaa guaaguggcu uguugaacac aucagggggu uuaagaaagc augcgaggau     2280

agccugaaga uucagaaaga caaccgugga uugcucaacc auaagaucaa uauugcccgg     2340

aacaccaaag guaaguguga gaaggaaauc uucaaucuga uaugcaaaau cgagggcucc     2400

gaagauaaga aagggaacua caagcacgga cuagcauaug agcugggcgu gcuccuguuu     2460

ggugaaccca augaggccuc uaaaccugag uucgaccgaa agauuaagaa auuuaacucg     2520

aucuacagcu ucgcucagau ucaacagauc gcguuugcag aaagaaaggg gaaugccaac     2580

acgugugcug ucugcuccgc cgauaaugca cauaggaugc agcaaauaaa aauuaccgag     2640

ccaguggaag acaacaagga uaaaaucauu cuuucagcca aggcucagcg cuugcccgcc     2700

aucccgacac ggauaguaga cggcgcaguu aagaaaaugg cgacuauccu ggcuaagaau     2760

auuguggaug acaacuggca gaauaucaaa caggucuuaa gugccaagca ccaacuccac     2820

auuccuauca uaaccgaguc uaacgcauuc gaauuugagc ccgcccuggc ugaugugaaa     2880

ggaaagagcc uuaaggacag aaggaaaaag gcccuggagc gcauuucccc agaaaauauc     2940

uucaaagaca agaacaaccg aaucaaagag uucgcaaagg gcauuucagc uuauagcggg     3000

gccaaucuaa cagauggaga cuuugauggu gcgaaagaag aacucgacca uaucauuccu     3060

cggucucaca agaaguacgg cacucugaac gaugaggcca auuugauaug cgucacccgu     3120

ggggacaaca aaaauaaggg aaacagaauc uucugucuga gggauuuagc ugacaauuau     3180

aaacucaagc aguuugaaac aaccgaugac cuggagauug agaaaaagau cgcagauacu     3240

auuugggacg ccaacaagaa agauuucaag uuuggcaauu accgcuccuu caucaaccuu     3300

acgccccagg agcagaaggc uuuucggcau gcccuguucu uggcagacga aaauccaaua     3360

aaacaagcgg ugaucagagc cauuaacaau aggaaccgaa cauuuguuaa ugguacccag     3420

cgcuacuucg cugaggugcu cgccaacaau aucuaucugc gggcuaaaaa ggagaaccua     3480

aacacugaca agauuaguuu ugauuacuuc gggaucccca ccauaggcaa uggaagaggc     3540

auugcugaaa ucaggcagcu guaugagaaa guagacagcg auauccaggc cuacgcaaag     3600

ggggacaaac cucaagccuc uuauucccac cuuauugaug cuaugcucgc guucugcaua     3660

gccgcagacg aacaucguaa cgauggauca aucggucugg agauugacaa gaauuacagu     3720

uuguauccac uggauaaaaa cacaggcgaa gucuuuacca aggacaucuu cagccagauu     3780

aagaucacug auaaugaguu uucggacaaa aaguuagugc gcaaaaaggc uauagagggg     3840

uucaacacac accggcagau gacgagagau ggaaucuacg ccgaaaauua ccucccgauu     3900

cugauccaca aagagcuuaa cgaaguuagg aagggcuaua ccuggaaaaa uuccgaggaa     3960

auuaagaucu uuaaggguaa aaaguacgac auacagcaac ugaacaauuu gguguauugu     4020

cucaaauucg ucgacaagcc cauuucuauc gauauccaga uuagcacucu ggaggagcua     4080

cgaaacaucc ugacaaccaa uaacauagcc gcuacagcag aauacuauua cauuaaucuu     4140

aaaacucaga agcuccauga guauuacauc gaaaacuaca acaccgcccu gggguauaag     4200

aaauacucaa aggagaugga guuuuugcgg aguuuagcgu aucgcuccga aagagugaaa     4260

auuaagucua ucgacgaugu aaaacaagug cuggacaagg auagcaauuu cauaauuggc     4320

aagaucacgc ucccuuuuaa aaaggagugg cagaggcugu accgggaaug gcagaacacc     4380

acaaucaaag acgauuauga guuccuuaag uccuuuuuca auguuaaauc aauuacuaag     4440

cugcacaaga aaguccgaaa ggacuuuagc uugccaauca guaccaacga aggaaaauuc     4500

cuagugaaga gaaaaacaug ggauaauaac uucauuuacc agauacugaa ugacucugau     4560

ucccgcgcug acggcacuaa gcccuuuauc ccugccuucg acauuagcaa aaacgagauc     4620

guggaggcaa ucauugauuc guuuaccuca aagaauauau ucuggcuccc aaagaacauc     4680

gaacugcaaa aagucgacaa uaagaacauu uuugccaucg auacgucuaa augguucgag     4740

guugaaacac ccagugaccu uagggauauu gggaucgcua ccauacagua uaagaucgac     4800

aauaacuccc guccgaaagu gcgggugaag uuagauuacg uaauugacga ugacagcaag     4860

aucaauuacu uuaugaacca uucucugcuc aaaagcagau auccugauaa ggucuuggag     4920

auucugaaac aguccacuau cauagaguuc gaaucaagug gauuuaacaa gacaauuaaa     4980

gagaugcucg gcaugaagcu ggcagguauc uacaaugaaa ccagcaacaa uaagaggcca     5040

gccgcgacua aaaaggcugg gcaggccaaa aagaaaaagu aaggacuagu cccuguuccc     5100

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     5160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     5220

uu                                                                    5222


<210>  3540
<211>  3494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3540
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaag aggaacuaca uucugggacu cgacauaggu      180

aucacaucag uuggcuaugg gauuaucgau uacgagacac gggacgugau ugaugccgga      240

guacgccuuu ucaaagaagc gaauguggag aacaaugaag gccgacguuc caagagaggu      300

gcuaggcggc ugaagcgcag aaggcggcau cgcauccagc gagucaaaaa guuguuauuu      360

gacuauaacc ugcuaacuga ucacucugag cucucaggga uaaauccaua cgaagccaga      420

gugaaaggac ugagucaaaa acuuagcgag gaggaguucu ccgcagcccu guugcaucuc      480

gcuaaaaggc ggggcguuca caacgugaau gaagucgaag aggacacggg caacgagcug      540

ucuaccaagg aacagaucuc gcguaauuca aaggcacugg aggaaaaaua uguggccgag      600

cuccaguuag aacgccugaa gaaagauggg gagguaagag gaagcauuaa cagguuuaag      660

acaagugacu acguuaaaga ggcuaagcag cuuuugaaag ugcaaaaggc cuaucaucag      720

cuggaucagu ccuucaucga cacuuacauu gaucuacucg aaacccggcg aacauacuau      780

gaggguccgg gcgaaggguc ucccuuugga uggaaggaca ucaaagagug guacgaaaug      840

cugaugggcc acugcaccua uuucccugag gagcuucgca gcgucaagua cgcguauaau      900

gcagaucugu acaacgccuu gaaugaccuc aacaaucugg ugauaacuag agacgaaaac      960

gagaaauuag aauauuacga gaaguuucag auuaucgaaa acguguucaa acaaaagaag     1020

aaaccaacac ugaagcagau ugcuaaagag auccucguca augaggaaga uaucaagggu     1080

uacaggguua ccuccacggg gaaacccgag uuuacuaacc uuaaggugua ucacgacauu     1140

aaggauauaa ccgcccggaa agaaaucauu gagaaugcag agcuguugga ccagaucgcu     1200

aagauacuaa caauuuacca gucaagugaa gauauccaag aggaacugac uaaccucaau     1260

agcgagcuga cccaggaaga gaucgagcag auuucuaacc uuaaaggaua uacaggcacc     1320

cauaaucugu ccuugaaggc caucaaccuc auucuggacg aauuauggca cacgaaugau     1380

aaccaaauag cgaucuucaa ucgccugaaa cuuguaccua agaaggugga ccucucacag     1440

cagaaagaga uuccaacuac acuggucgau gacuuuaucc uuagccccgu gguuaagaga     1500

aguuucaucc agucuauuaa agugauaaac gccaucauua agaaauacgg cuugccuaau     1560

gauaucauua ucgaacuggc aagggagaag aacuccaaag acgcucaaaa gaugauaaau     1620

gaaaugcaga agcguaaccg gcagaccaac gagcgaaucg aggaaauuau cagaacuaca     1680

gggaaagaga augccaagua ucucauugaa aagaucaagc ugcaugacau gcaggaggga     1740

aaaugucuuu acagccugga agcuauacca uuggaggauc uccugaacaa uccguuuaac     1800

uaugaggucg accacauuau ccccaggucg gugucuuucg auaauuccuu caacaauaag     1860

guauuaguua agcaagaaga gaacucaaag aaggguaauc gcaccccuuu ucaguaccug     1920

aguagcuccg acucuaaaau uagcuacgaa acguucaaga aacacauccu aaaccucgcc     1980

aagggcaagg ggcggauauc aaaaaccaag aaagaguauc ugcuugagga aagagauauc     2040

aauagguuua gugugcagaa ggacuucauu aaccgcaauu uggucgauac acgauacgca     2100

acucggggac ugaugaaccu ccugcguucc uauuuuagag ugaauaacuu agacgucaaa     2160

gugaaaagca ucaacggcgg uuucaccucu uuucugaggc gcaaguggaa auucaagaaa     2220

gagcggaaua agggguacaa acaucacgcc gaagaugcuc uuauuaucgc gaacgcagac     2280

uuuauauuca aggaguggaa aaaguuggau aaggccaaaa agguuaugga aaaucagaug     2340

uuugaggaga aacaagcuga aucaaugcca gagauugaaa cagagcagga auauaaggag     2400

aucuucauca cuccccauca gauuaaacac aucaaggacu uuaaggauua caaauauagu     2460

cauagagugg acaagaaacc uaacagggag cucauuaaug acacccugua cagcacacga     2520

aaggaugaca aaggcaacac ccuaauagua aauaaccuga auggacucua cgauaaggac     2580

aacgauaagc ugaaaaagcu aaucaauaaa uccccagaaa aguugcugau guaucaccac     2640

gacccccaga cuuaccaaaa auuaaagcuc auuauggagc aguauggcga ugaaaagaac     2700

ccgcuguaca aauauuacga ggaaacgggg aauuaucuua caaaguacuc uaaaaaggac     2760

aacggaccug ucaucaaaaa gauuaaguac uaugguaaua aacugaacgc ccauuuggau     2820

aucaccgacg auuaccccaa cucgcgcaau aaggugguua aacucagccu gaagccauau     2880

cgguucgacg uguaccuaga uaacggcguc uauaaauucg ugacuguaaa gaaucuggac     2940

gugaucaaaa aggagaacua cuaugagguu aauuccaagu gcuacgaaga ggccaaaaag     3000

cucaaaaaga uaucaaacca ggcagaauuu auugcuaguu ucuacaauaa cgaccugauc     3060

aaaauuaaug gggagcuuua uagagucauc ggagugaaca augauuugcu gaacaggaua     3120

gaggugaaua ugauugacau caccuaccgu gaauauuuag agaacaugaa ugauaagcgc     3180

ccuccccgga ucauuaagac aauagccucu aaaacccaaa gcaucaagaa auacuccacu     3240

gacauucucg gcaaccugua ugaagucaag agcaaaaagc acccacagau cauuaagaaa     3300

gguaagagac ccgcagcgac caaaaaggcu gggcaggcca aaaagaagaa auaaggacua     3360

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     3420

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaag aauu                                                       3494


<210>  3541
<211>  4562
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3541
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcacug uucaacaagu gcauuauaau cagcauuaau      180

cucgacuuuu ccaacaaaga gaaguguaug accaagccau acucuaucgg acuugauauu      240

gguacaaauu caguuggcug ggccgugauc acugacaacu auaaaguacc gaguaagaaa      300

augaaggugc uggggaauac gagcaaaaag uacauaaaga aaaacuuguu aggaguccug      360

cuauucgauu ccggcaucac cgcggaaggu aggcggcuca agcgcacagc ucgacguaga      420

uauacuaggc ggcgcaauag gauucuguac cuucaggaga ucuuuucuac cgaaauggcc      480

acacuggacg augcauucuu ucaaagauug gacgauucgu uccucgugcc cgacgauaaa      540

cgggacucaa aguauccuau uuuugggaac cugguugagg aaaaagugua ccaugaugag      600

uucccaacca ucuaucaucu gcgcaaguac cucgccgaca gcacuaaaaa ggcugauuua      660

cgacuggucu accuugcauu ggcccacaug auaaaguaua gaggacacuu ucugauugag      720

ggcgaguuca auaguaaaaa caaugacauc cagaagaacu uucaggacuu ccuagauaca      780

uacaaugcua uuuuugaguc cgaccucucu cuggaaaaca gcaaacagcu ugaggagauc      840

gugaaggaua aaaucuccaa gcuggaaaag aaagacagga uuuugaaacu cuuccccggc      900

gagaaaaauu cagggauauu cagugaauuu cugaaguuaa ucguaggaaa ccaagccgau      960

uuccggaaau gcuuuaaccu ggacgagaag gcgagccucc auuucucuaa ggaauccuau     1020

gaugaggacc uugagacccu guuggguuac auuggcgaug acuauucaga uguuuuucua     1080

aaagcaaaga aacuguacga cgccauccuc cugagcgggu uccuuacggu gacugacaau     1140

gaaaccgagg cuccucugag uucugccaug auaaagcguu auaacgaaca caaagaggau     1200

uuggcacucc ugaaggaaua cauucgcaau aucuccuuaa agacauacaa cgaggucuuu     1260

aaagacgaua cuaagaaugg auaugcuggc uacaucgacg guaaaaccaa ccaggaggau     1320

uucuaugugu accugaagaa ucuacucgcc gaauuugagg gggcggacua uuuccuggaa     1380

aagauugaua gagaggacuu ucuuaggaag cagcggacau ucgauaacgg aagcauccca     1440

uaccagauuc acuugcaaga aaugcgagcc auacuggaca aacaggcaaa guuuuauccc     1500

uuccucgcua agaauaaaga gcgcaucgag aagauucuga ccuucagaau cccuuacuac     1560

gugggcccac uugccagggg caacucggau uuugcuuggu cuauccggaa acgcaaugaa     1620

aagauuacgc cguggaacuu cgaggacguc auagauaaag aauccucagc cgaggcauuu     1680

aucaauagaa ugacaaguuu cgaccuguau uugcccgagg aaaagguucu cccuaagcau     1740

agccuguuau acgagacuuu uaacguguau aaugaacuga ccaaaguaag guucauugcc     1800

gaguccaugc gugacuacca guuucuagau ucuaagcaga aaaaggacau cgugcggcuc     1860

uauuucaaag auaagcgaaa ggucacugac aaagauauua ucgaauaccu gcacgcuaua     1920

uauggguacg acggaaucga gcuuaagggu auugagaaac aauuuaacag cucauugagu     1980

acauaccaug aucugcucaa caucauuaau gacaaggagu uccuggauga cuccagcaac     2040

gaagcgauca uagaagagau uauccacacc uuaacgauuu uugaagauag agagaugauc     2100

aaacagaggc ugucuaaguu cgagaauaua uuugacaagu cagugcuuaa aaaguugagu     2160

cgccggcacu auaccggcug ggggaaacuc agcgcaaagc ugaucaacgg aauuagagau     2220

gaaaaauccg gcaauacaau ccuagacuac cugauugacg augguaucuc uaacaggaau     2280

uucaugcagc ucauacauga cgaugcccug ucguucaaga aaaagauuca aaaggcucag     2340

aucaucgggg acgaggauaa aggcaacauu aaggaaguug ugaaaagccu uccaggaucc     2400

cccgccauca agaaaggcau uuugcaguca auaaagaucg ucgacgagcu ggugaaggua     2460

augggagggc gcaaaccuga aaguauuguu guggagaugg cacgagagaa ucaguauacu     2520

aaccaaggua agucuaauag ccagcagcgg uuaaaacguc ucgaaaaguc ccugaaagag     2580

cuuggcucaa agauccugaa ggaaaacauu ccagccaaau ugagcaagau cgauaauaac     2640

gcucuccaga augacagacu guaccuauau uaccugcaaa acgggaaaga uauguauacc     2700

ggagacgauc ucgacauaga caggcugucu aacuacgaua ucgaccacau uaucccccag     2760

gccuuucuua aggauaauuc cauugacaac aaagucuugg ugaguagcgc aucuaaucgc     2820

ggcaaguccg augacuuccc gucacuggag gucgugaaga aacggaagac auuuugguac     2880

caguuacuca aaaguaagcu gaucagccag agaaaauucg auaaccuuac uaaggcggag     2940

aggggugggc ugcuaccuga agacaaggcu ggcuuuauac aacgacagcu cguugagacc     3000

cgccagauua caaaacaugu ggcccggcug uuggaugaaa aguucaauaa caaaaaggac     3060

gagaauaaca gagcaguaag gaccgucaaa aucaucacuc ugaagucgac guuagugucc     3120

caguuucgua aagauuucga acucuauaag guucgcgaga uuaaugacuu ucaccaugcc     3180

cacgaugcuu accugaacgc cgugauagca ucugcccuuc ugaagaaaua ucccaaguug     3240

gagccagagu ucgucuacgg agacuauccu aaauacaaua gcuuucggga aagaaaguca     3300

gcuacagaaa aaguguauuu cuacaguaac aucaugaaua uuuucaagaa guccaucucu     3360

cucgcggacg gcaggguaau ugagcgaccc cugaucgaag ugaacgagga gaccggggaa     3420

agcguuugga auaaagaguc cgaucuagcc acuguccggc gcgugcuguc auacccacaa     3480

gugaacgucg uuaagaaagu ggaagaacag aaccacggac uugacagagg uaagcccaaa     3540

ggccucuuua augcuaaccu gagcaguaag ccuaagccaa auucuaacga aaauuuggug     3600

ggggcaaaag aguaucugga uccgaagaaa uacggaggcu augccgguau auccaacagc     3660

uucgcuguau uagucaaggg gaccaucgag aaaggcgcca agaagaaaau uacaaaugug     3720

cucgaauuuc agggaaucuc gauucuggac aggaucaacu accggaagga uaaacuuaau     3780

uuccuguugg agaagggcua uaaagacaua gaacucauua ucgagcugcc caaguacuca     3840

cuauuugagc ugucugaugg gagucgaaga augcuugcau ccauccucag caccaacaau     3900

aaacgcggag aaauucauaa gggcaaccaa aucuuccugu cucagaaguu uguuaaauug     3960

uuauaucacg cgaagaggau aagcaauacu auuaacgaga aucaucguaa auacguggaa     4020

aaccacaaga aagaguucga ggaacuguuu uacuauaucc ucgaguucaa ugaaaacuac     4080

gucggugcca agaagaacgg gaaacugcuu aauuccgcuu uucagucaug gcagaaccac     4140

aguauugacg agcuguguag cucuuucauc ggaccuacag gcuccgaacg gaaggguuug     4200

uuugagcuaa cgucaagagg cagugccgca gauuucgagu uccugggggu gaaaauacca     4260

agguaucgcg acuacacccc cagcucccuc cugaaggaug cuacucuuau ucaucaaucu     4320

guuacaggau uauaugaaac ccgaaucgac cuggccaaac ucggcgaggg gaagagaccu     4380

gcagccacaa agaaagcugg acaggcgaag aaaaagaaau aaggacuagu cccuguuccc     4440

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     4560

uu                                                                    4562


<210>  3542
<211>  3638
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3542
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaugg uacgccagcc ugauguccgc gcaucaccuc      180

aggguuggaa uugacguggg uacccauucu guaggccuug cuacacugcg gguggaugac      240

cacgggacuc caauagaguu guuaucagcc cugagucaua uccacgauag cggagucggc      300

aaggaaggua aaaaggacca cgauacgcgc aagaaacuau ccgggauugc acgacgugcc      360

agaaggcucc ugcaucaccg gcgcacccag cuucaacagc uggacgaggu guugagagau      420

cucggauucc cgauccccac accuggcgaa uuucuggacc ugaacgagca gacugaucca      480

uauaggguuu ggcgggugcg cgcucgacuc gucgaagaga aguuacccga ggaacugaga      540

ggcccugcaa uuucuauggc cgugaggcau aucgcucggc accgugggug gcgcaaucca      600

uacucgaaag uagagucacu uuugagcccc gccgaagaga guccuuucau gaaggcgcug      660

agagagagga uacuagcaac cacaggagaa guucucgacg augguaucac cccaggccag      720

gccauggcuc aaguggcccu gacucacaac auuuccaugc gggggccgga gggaauccuu      780

ggcaaacugc aucagucuga caaugcaaac gaaauucgaa agaucugcgc ucgccagggu      840

gucagccccg auguguguaa gcaguugcuc agagccgugu uuaaagcgga cuccccuagg      900

gggucagccg ucagucgggu ugcaccagac ccccugccug gacaaggcag cuuccgcaga      960

gcuccaaagu gcgaucccga guuucagagg uuccguauaa uuucuaucgu ggccaauuua     1020

cggauuuccg aaacaaaagg cgagaaccga ccgcugaccg cugacgagag aaggcacgua     1080

gugacguuuc ucacugaaga uucacaggcc gaccuuaccu gggucgaugu ggcagagaag     1140

cuggggguuc aucgccggga cuugagagga acagccgugc acacugauga cggugaaagg     1200

agcgcugcgc gcccucccau cgaugcaacc gaccgaauca ugcggcagac aaaaauuagu     1260

ucucuaaaga ccugguggga ggaagccgau uccgagcaac guggcgcuau gauaagauau     1320

cuguacgagg acccaacgga cagcgaaugu gccgagauca uugcagaacu cccugaggaa     1380

gaucaggcca aacuggacuc gcuucaucug cccgcuggga gggccgcaua uucucgcgag     1440

uccuugacug cgcucucaga ucacaugcug gcuacaaccg acgauuuaca cgaggcccgg     1500

aagagacugu ucggagucga cgauaguugg gcaccacccg ccgaagcuau caaugccccu     1560

gugggcaacc caagcguaga caggacucua aagauaguug gucgauaccu cuccgcagug     1620

gagucuaugu gggggacacc ggaagucauu cauguggagc acguccgcga uggcuuuacc     1680

agcgagcgga uggcugacga aagagauaaa gccaauaggc gucgcuauaa cgacaaucag     1740

gaggcgauga agaaaaucca acgggauuac ggaaaggaag gcuacaucuc aagaggggac     1800

auugugaggc uggacgcccu ugaguugcag ggaugcgcuu gucuguauug cgguacgacc     1860

aucggcuacc auacauguca gcucgaucac auuguucccc aggcagggcc uggaaguaac     1920

aaucgacggg gcaaccuggu ggccguaugc gaacgcugua auagauccaa aagcaacacu     1980

ccauucgcug ucugggccca aaagugcggu auaccccacg uggggguuaa ggaggcaauc     2040

ggcagggugc ggggauggcg aaaacagacc ccuaacacau cuucagagga ccuuacuaga     2100

cugaagaaag aagucauugc gcgcuugagg cguacccagg aggauccaga aaucgacgag     2160

cggaguaugg aaagcguggc cuggauggcu aaugagcucc aucacagaau cgccgcagcu     2220

uaucccgaga caaccguaau gguguacagg ggcuccauua cugccgcagc ccgcaaggcu     2280

gcggggauag auucucgaau caaccugauu ggagaaaaag guagaaagga ccggaucgau     2340

aggcgccauc acgccguuga cgcaucgguc guggcuuuaa uggaggccag cguggccaag     2400

acgcuggcug aacguuccuc acuaagaggc gagcagcggc ucacagggaa agaacaaacc     2460

uggaagcagu auacuggaag uaccgucggc gcaagggagc auuuugagau guggcgcggu     2520

cacaugcugc accuuacaga auuguucaau gagcgacugg ccgaagauaa aguuuacgug     2580

acccagaaca uuagacuccg gcugucugac gggaaugcgc auacugugaa cccgagcaag     2640

uuaguauccc acaggcuggg cgauggacuu acaguccagc aaaucgaccg cgcuuguacg     2700

ccugccuugu ggugcgcacu caccagagag aaagauuuug acgagaagaa uggccugcca     2760

gccagggaag accgggcuau acgugugcau gggcacgaga ucaagucaag cgauuauauu     2820

cagguuuucu cuaaacgcaa gaaaacugac uccgaucgag acgaaacacc cuucggagca     2880

aucgccguga gaggcgguuu ugucgagauu gggccuagua uccaucacgc gaggauauac     2940

cggguggaag gaaagaaacc cguuuacgcu augcuacgcg uguucaccca cgaucugcuc     3000

agccagagac auggcgaccu guuuucugcc guaauuccac cucaguccau cucaaugagg     3060

ugcgcagagc ccaaacuucg gaaagccauu acaacuggua acgcuaccua uuugggcugg     3120

gucguggugg gggaugagcu ggaaaucaau gucgacaguu ucacgaagua cgccauagga     3180

cgauuuuuag aggauuuccc aaacaccaca agauggcgca ucuguggcua ugacacuaau     3240

agcaagcuca cccugaaacc gauuguucuu gcagcugaag ggcuggagaa cccuucgucc     3300

gccgugaaug aaaucguaga guugaaggga uggagggugg cgauuaacgu ccucacaaaa     3360

gugcacccca cuguugugcg gcgugaugcc cugggcagac caagguacuc uagccgcuca     3420

aaucuaccca ccaguuggac gaucgagaag cggccugcag cuacaaagaa ggccggucaa     3480

gcuaagaaaa agaaauaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu     3540

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                             3638


<210>  3543
<211>  4499
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3543
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaacc aagccauaca gcauuggacu ggacauaggu      180

acaaacuccg uuggcugggc cgugacuacg gauaauuaua aaguaccguc uaagaagaug      240

aaagugcucg ggaacaccuc aaagaaauac aucaagaaaa aucuucuggg agucuuguua      300

uucgacagug gcauuacagc ggaggguagg cggcugaagc gcacugcucg acguagauau      360

accaggcggc gcaacagaau ccuauaccuc caggaaauuu uuagcacaga gauggccacc      420

cuggaugacg cauucuuuca aaggcuugau gacuccuucc uggugcccga ugacaagcgg      480

gauucuaaau auccuaucuu ugggaauuug guugaagaga aggccuacca ugacgaguuc      540

ccaacuauau aucaccuccg caaauaccug gcugauucga caaagaaagc agaccugcga      600

cucguguacu uagcccuggc ucauaugauc aaguauagag gacacuuucu uauugagggc      660

gaguucaacu caaagaauaa cgauauccag aagaauuuuc aggacuucuu ggacaccuac      720

aacgccauuu uugaaagcga ucugagucua gaaaauucca agcagcucga agagaucguc      780

aaagacaaga uaucuaaacu ggagaagaaa gauaggauuc uuaagcuguu ccccggcgaa      840

aagaacagcg ggaucuuuuc cgaguucuug aaacucauug ugggaaauca agcggacuuc      900

cggaagugcu uuaaccugga ugaaaaagca ucauuacauu ucaguaagga gagcuaugac      960

gaagaucugg agacgcuccu ugguuacauc ggcgacgauu auucugacgu auuucugaaa     1020

gccaagaagu uguacgaugc uauccuacug uccggguucc ucacuguuac cgacaaugag     1080

acagaagccc cucugucaag cgcaaugauu aaacguuaua acgagcacaa ggaagaccuu     1140

gcucuguuga aagaguacau acgcaacauc agucucaaga cuuacaauga aguguuuaaa     1200

gaugacacca agaacggaua ugccggcuac auugauggua agacaaauca ggaggacuuc     1260

uaugucuacc ugaaaaaguu acuggcggag uuugaagggg ccgauuauuu ccuagagaaa     1320

aucgacagag aagauuuucu caggaagcag cggaccuucg acaacggauc uauaccauac     1380

cagauucacc uucaagagau gcgagcaauc cuugauaaac aggcuaaguu uuaucccuuc     1440

uuggccaaaa auaaggaacg caucgagaag auucugacgu uuagaauccc uuacuacgug     1500

ggcccacucg cuaggggcaa cuccgacuuc gccuggagca uucggaaacg caaugagaag     1560

auaacuccgu ggaacuucga agaugugauc gacaaagagu cgucugcaga agccuuuauu     1620

aauagaauga cauccuucga ucuguaucuu cccgaggaga agguccugcc uaaacauuca     1680

uugcucuacg aaaccuuuaa cguuuauaau gagcugacua aggugagguu caucgcugaa     1740

aguaugcgug acuaccaguu uuuagacagc aagcagaaaa aggauaucgu acggcuguau     1800

uucaaagaca agcgaaaagu gacagauaag gacauuauag aguaccuaca cgcgaucuau     1860

ggguacgaug gaauugaacu caaggguauc gagaaacaau uuaacuccuc ucugagcacc     1920

uaccaugacc uuuugaauau uaucaacgau aaggaguucc uggacgauuc aaguaaugaa     1980

gcaauaaucg aggaaauuau ccacacgcuc accauuuuug aggacagaga aaugaucaaa     2040

cagaggcugu ccaaguucga gaacauauuu gauaaaagcg ucuuaaagaa gcugucucgc     2100

cggcacuaua caggcugggg gaaacuuuca gccaaguuga uuaacggaau cagagacgag     2160

aaaaguggca auacuauucu cgauuaccug aucgacgacg guauaagcaa caggaauuuc     2220

augcagcuaa uccaugauga cgcucugucc uuuaagaaaa agauucaaaa agcccagauc     2280

auuggggaug aagacaaggg caacaucaag gaggugguua aaucucuccc aggaucgccc     2340

gcaauaaaga aaggcauucu gcagagcauc aagaucgugg augaacuugu caaagugaug     2400

ggagggcgca agccugaguc cauuguaguu gaaauggccc gagagaauca guauaccaac     2460

caagguaagu caaauaguca gcagcgguug aaacgucugg agaagucuuu aaaagaacuc     2520

ggcagcaaga uccugaaaga gaacauucca gcuaagcugu ccaagauaga caauaacgcc     2580

cugcagaaug auagauugua ccucuauuac cugcaaaacg ggaaagacau guauacagga     2640

gaugaccuag auaucgacag gcugucaaau uacgacauug aucacaucau uccccaggca     2700

uuccucaagg acaacagcau cgauaauaaa gugcuggucu cuuccgcgag uaaccgcggc     2760

aagagcgacg augugccguc ucuugaaguc gugaaaaagc ggaagacuuu cugguaccag     2820

uugcugaaau ccaaguuaau aucacagaga aaauuugaca accucaccaa ggcugagagg     2880

ggugggcuga guccugagga uaaagccggc uucauccaac gacagcuugu ugaaacacgc     2940

cagauuacca agcauguggc acggcugcua gacgagaagu uuaauaacaa aaaggaugaa     3000

aauaacagag ccguaaggac ugucaaaauc auuacgcuca agagcacacu ggugucgcag     3060

uuccguaaag acuuugaguu guauaagguu cgcgaaauca augauuucca ccaugcucac     3120

gacgccuacc ugaacgcagu ggucgcuucc gccuuacuca aaaaguaucc caagcuggag     3180

ccagaguuug uguacggaga uuauccuaaa uacaauucuu uccgggaaag aaagagcgcg     3240

accgagaaag uauauuuuua cucaaacaua augaauauuu ucaagaaaag uaucucccuu     3300

gccgacggca gggugaucga acgaccccug auugagguua acgaagagac uggggagucu     3360

gucuggaaua aggaaagcga cuuggcuacc gugcggcgcg ugcucuccua cccacaaguc     3420

aacguuguga agaaagugga ggaacagaau cacggacugg auagagguaa gcccaaaggc     3480

cuauuuaacg caaaucuguc aagcaagccu aaaccaaaca guaacgagaa ucuuguaggg     3540

gccaaggaau aucucgaccc gaagaaauac ggaggcuaug cugguauauc uaacuccuuc     3600

acaguccugg ugaaggggac caucgagaaa ggcgccaaga aaaagauuac uaauguuuug     3660

gaguuccagg gaaucagcau ucuggauagg aucaacuacc ggaaggacaa auuaaauuuu     3720

cuccuggaaa agggcuauaa agauauagag cuuaucauug aacugcccaa guacucguug     3780

uucgagcucu cagacggguc ucgaagaaug cuggcaagua uccuauccac aaacaauaaa     3840

cgcggagaga uucauaaggg caaccaaauc uuucugagcc agaaauucgu gaaacuucuc     3900

uaucacgcga agaggauauc uaauacgauu aacgaaaauc aucguaaaua cgucgagaac     3960

cacaagaaag aauuugagga acuguucuac uauaucuugg aguuuaauga gaacuacgug     4020

ggugccaaga aaaaugggaa guuacugaac agcgcuuucc aguccuggca gaaucacuca     4080

aucgaugaac ucuguaguag cuuuauugga ccuaccggcu cugagcggaa gggucuguuc     4140

gaacuuacuu ccagaggcuc agccgcagac uuugaguucc uggggguuaa aaucccaagg     4200

uaucgcgauu acacacccag uagcuugcua aaggacgcua cccugauaca ucaauccgug     4260

acaggacucu augaaacucg aauugaucug gccaaacuug gcgaggggaa gagaccugca     4320

gccaccaaaa aggcuggaca ggcgaagaaa aagaaauaag gacuaguccc uguucccaga     4380

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      4499


<210>  3544
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3544
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcagcc gcguucaagc caaacagcau uaauuacaua      180

cugggacucg acaucgguau ugcuuccguu ggcugggcca ugguggagau cgaugaagag      240

gaaaacccga uuaggcuuau cgaccugggg guacgggugu uugagcgcgc agaagucccc      300

aaaaccggag auucuuuggc cauggcucga cguuuagcaa gaucagugag gcggcugaca      360

cgcagaaggg cccaucggcu acuccgcacu cgaagacugc uuaagaggga gggcguucug      420

caggcugcca auuucgacga gaacgguuug auaaagaguc ucccuaauac gccauggcaa      480

cugcgggcgg cagcccugga ucguaaacuc acccccuuag aauggagcgc ugugcugcuu      540

cacuugauca agcaucgcgg guaucugucc cagaggaaaa acgagggaga aacagccgac      600

aaggagcuag gcgcacuccu gaaaggcguc gcugggaaug cccacgcgcu ucagacugga      660

gauuuuagga ccccugccga gcuggcauug aacaaguucg aaaaggaguc uggucauauu      720

cggaaucagc gaucggacua cucacacaca uuuagccgca aagaucucca agcugaacug      780

aucuuacugu ucgagaagca gaaagaauuu ggcaacccac acgugagugg gggacucaag      840

gagggcauug agacccuucu gaugacucag agacccgccu uguccgguga cgcuguacag      900

aaaaugcuag ggcauugcac auucgaaccu gccgagccaa aggcagccaa aaauaccuau      960

acggcugaaa gguuuaucug gcugacuaag cucaacaauc ugcggauacu ugagcaagga     1020

ucugaacgcc cgcugaccga uacagagaga gcgacuuuga uggacgagcc cuacagaaag     1080

agcaaacuca ccuaugcaca ggcccguaag cuguuaggcc uggaagauac agcuuucuuu     1140

aaaggccuac gguacgggaa ggacaacgcc gaggcaucca cccucaugga aaugaaagcc     1200

uaucacgcua uuucacgagc ccuggagaag gaaggacuua aggacaaaaa gaguccuuug     1260

aaucugagcc cagagcucca ggaugagauc gguacggcau ucucucuguu uaaaacugac     1320

gaagauauua caggcagacu uaaggacagg auccagcccg agauccugga agcguugcuc     1380

aaacauauuu ccuucgauaa guucguucaa auaucacuga aggcuuuacg ccggaucgug     1440

ccucugaugg agcaggggaa aagauacgac gaggccugug cagaaauuua cggagaucac     1500

uauggcaaga aaaacaccga ggaaaagauc uaccuaccac ccauaccggc cgacgagauu     1560

aggaacccug ucgugcuccg cgcucugagc caggcccgaa aagugaucaa uggugucguu     1620

cggcguuaug ggagucccgc aagaauccac auugaaacug cuagggaggu gggcaagucu     1680

uuuaaggauc gcaaagagau cgaaaagcgg caagaggaaa acagaaaaga cagggagaag     1740

gccgcggcca aauuccgaga auacuuucca aauuucguag gagagccuaa guccaaagau     1800

auucuuaagu ugcgccugua ugagcagcag cauggcaagu gccucuacag cgggaaagaa     1860

auaaaccugg gacgguuaaa ugagaagggu uauguggaaa ucgaccacgc ucugcccuuu     1920

ucgagaacau gggacgauuc uuucaacaau aaaguccuug uguugggcuc cgagaaccag     1980

aauaagggga accaaacccc auacgaauac uuuaauggaa aagacaacuc aagggagugg     2040

caggaguuca aggcacgugu ugaaacgagu cgcuuucccc ggagcaagaa acagaggauu     2100

cuccugcaga aguucgauga ggacggcuuu aaagaaagaa aucuaaacga uacccgauau     2160

gugaaucggu uccuguguca auuugucgcc gaccgcauga gacucacagg uaaggggaaa     2220

aagagggugu ucgcuuccaa cggccagauc acuaaccugc uucggggauu cuggggcuug     2280

cgaaagguaa gagccgagaa ugaucgccau cacgcacugg acgcgguugu ggucgccugc     2340

ucuaccgugg cuaugcagca gaaaaucaca agguuugucc guuacaagga gaugaacgcc     2400

uucgauggga aaacuauuga caaggaaacc ggagaggugu uacaucagaa aacacacuuu     2460

ccucagccau gggaguucuu ugcacaggaa guuaugauac ggguguucgg uaagccggau     2520

ggcaagcccg aauuugagga ggcugacacc cucgaaaaac ugagaacucu ucuggccgag     2580

aaguugagcu caaggccuga agcaguacac gaguauguca cgccacucuu cgugagucgc     2640

gcccccaauc gaaaaauguc cgggcaggga cauauggaaa caguuaagag cgcuaaaaga     2700

cuggaugagg gcgugucugu ccuacgggug ccucugaccc aacucaagcu gaaagaccuu     2760

gagaagaugg uaaacaggga acgcgagcca aaguuguacg aagcgcugaa agcccguuua     2820

gaggcacaca aggacgaucc cgcuaaagcc uuugccgaac cguucuauaa guacgacaaa     2880

gcugguaaua gaacucaaca ggugaaggca guucgggucg agcaagugca gaagaccggg     2940

guguggguca ggaaccauaa uggcaucgcc gauaacgcga caaugguucg cguggacgug     3000

uuugagaaag gagauaagua uuaccucgua ccuauuuacu cauggcaggu cgcuaaaggc     3060

auccugccag accgagccgu gguucagggg aaggaugaag aggacuggca acuuauugau     3120

gacaguuuca auuuuaaauu cagccugcac cccaacgauc uaguggaagu caucaccaag     3180

aaggcaagaa uguucggaua uuuugccucc ugucaccggg gcacugguaa uauaaacauc     3240

aggauucaug accucgauca caaaaucggg aagaauggca uucuggaggg aaucggugug     3300

aaaacagcuu ugucuuucca gaaguaccag auagacgagc ugggcaaaga aauucgcccu     3360

ugcagauuaa agaagaggcc cccaguucgg aaacguccug cagccacgaa gaaagcgggg     3420

caggcuaaga aaaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3480

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  3545
<211>  4337
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3545
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaag aaaccauaca ccauuggacu ggacauaggu      180

acaaacagcg uuggcugggc cgugcucacu gaucaguaug accuuguaaa gaggaagaug      240

aaaaucgcgg gggauuccga gaagaaacaa auuaagaaaa auuucugggg agugcggcug      300

uuugacgaag gccagacggc ugccgaucgc cgaauggcac guaccgccag aaggcggauc      360

gagcgcagaa ggaaccggau uucuuacuug caggguaucu ucgcugaaga gaugucaaag      420

acagacgcaa acuuuuucug ccgcuuaagu gauagcuuuu augucgacaa ugaaaagcga      480

aauuccagac auccguucuu ugccacuaua gaggaggaag uggaguacca caaaaacuau      540

cccaccaucu accaucugag ggaggagcua guuaauucuu cggaaaaggc ugaucuccgg      600

cugguguauc uugcccuggc gcacauuauc aaauaccgug ggaacuucuu gauugaagga      660

gcacucgaca cacagaauac cucagucgau ggcaucuaca agcaauuuau acagacuuau      720

aaccaggugu ucgccagcgg cauugaggac gggagucuga aaaagcugga agauaauaaa      780

gacguagcua agauccucgu ugagaaggug acacgcaaag aaaaguuaga gaggauucug      840

aaacuuuacc cuggagagaa guccgccggu auguuugcac aguucaucuc uuugaucguc      900

ggcagcaaag ggaacuuuca aaagccauuc gaccugauug aaaaguccga uauagagugu      960

gcuaaagacu cauaugaaga ggaucuagaa agucuccugg cccuuaucgg agacgaguac     1020

gcggagcugu ucguggccgc aaagaaugcu uauagcgccg uggucuuguc uuccauuauc     1080

accguugcug aaacggagac uaacgccaaa cucucagcaa gcaugauaga aagguuugau     1140

acccaugagg aagaccuggg cgaguuaaag gccuucauua aacugcaccu ccccaagcac     1200

uacgaggaaa ucuuuaguaa uacagagaag caugguuaug cuggguacau cgauggaaaa     1260

acuaagcagg cggacuucua caaauauaug aagaugaccc uugaaaacau ugagggcgca     1320

gauuacuuua ucgccaaaau ugagaaggaa aacuuccugc ggaagcagcg aacauuugac     1380

aauggcgcua uaccucacca guugcaucua gaggaacugg aggccauccu ccaccaacag     1440

gcaaaauauu acccauuccu gaaggaaaac uaugauaaaa uuaagucucu ugugaccuuu     1500

cgcauccccu acuucguagg gccucuggcc aauggacagu ccgaguuugc uugguugacg     1560

agaaaagccg acggugagau caggccaugg aacauugaag agaaggugga cuucggcaaa     1620

agcgcagucg auuuuauaga aaagaugacu aauaaggaca cauaucuccc gaaagagaac     1680

gugcugccca agcacucguu augcuaccaa aaauaccugg uuuauaauga acuaaccaag     1740

gugcgguaca ucaacgauca ggggaaaacu ucuuauuucu ccggacagga gaaggagcag     1800

auuuucaaug accucuuuaa gcaaaaacgc aaggucaaaa agaaagaucu ggaacuuuuc     1860

uugagaaaca ugucacaugu ggagaguccu acaaucgaag gccuggagga cagcuuuaau     1920

uccucuuaca gcaccuauca cgaucuccug aagguaggua uuaagcagga aauccuugac     1980

aacccaguua auacggagau gcuggagaac auagugaaaa ucuugaccgu cuucgaagau     2040

aagaggauga uuaaagagca gcuccagcaa uuuucagacg ugcuggaugg ggucguguua     2100

aagaaacugg aacgucggca uuacacaggc uggggacgac uaagugcgaa gcuccugaug     2160

ggcaucagag acaagcaguc ccaccuuacu auuuuggauu aucugaugaa ugacgacggg     2220

cucaacagga accugaugca guuaaucaau gauagcaacc ugucuuucaa aucaauaauu     2280

gagaaggagc agguuaccac agcugacaaa gauauccaaa guauuguggc cgaccuugca     2340

ggaagccccg ccaucaagaa agguauauug cagucccuca agaucguaga ugaacugguc     2400

ucugugaugg gcuacccucc acagacuauu guuguggaga uggcucgcga aaaucagacc     2460

acagggaaag gaaagaacaa uucgcggccc agauacaaga gccuagagaa agccaucaag     2520

gaauuuggcu cccaaauucu gaaagagcau ccgaccgaca accaggagcu caggaauaac     2580

cgccuguauc uuuacuauuu gcagaauggu aaggauaugu acacugggca agaccuggau     2640

auccacaacu uaucaaauua ugacauagau cacauugucc cucagaguuu caucacggac     2700

aacucuaucg auaaucucgu gcugacaagc uccgcaggca accgagaaaa aggagacgac     2760

guacccccac uugagauugu gcggaagcgu aaggucuuuu gggaaaaacu guaccagggc     2820

aauuugaugu caaagagaaa auucgauuau cucaccaagg cugagagagg gggacugacu     2880

gaagccgaca aagcgcgcuu uauccaucgg cagcuaguug agaccagaca aauuacaaag     2940

aacguggcca acauacugca ccagagguuc aauuacgaga aggaugacca ugguaacacc     3000

augaaacagg ugcgaaucgu cacucucaag agcgcucugg uuucucaguu ucgcaaacaa     3060

uuccagcuuu acaaggugcg ggaugugaau gacuaucacc acgcacauga ugccuacuug     3120

aacggcguag ucgcuaauac acuguuaaaa guguauccuc agcucgaacc cgaguucguu     3180

uacggggacu aucaccaguu ugauugguuc aaggccaaca aggcaacggc gaaaaagcaa     3240

uuuuacacca auauuaugcu guucuuugcc cagaaagaca gaaucauuga ugaaaacgga     3300

gagauccuuu gggacaagaa auaucuggac acugugaaga aggucauguc cuacaggcag     3360

augaauauag ugaaaaagac agaaauccaa aaaggcgagu ucaguaaggc uaccauuaaa     3420

ccaaagggua acagcucuaa acuaaucccc cguaagacaa auugggaucc uaugaaguac     3480

gggggccucg acuccccaaa cauggccuau gcaguuguga uugaguacgc uaaaggaaag     3540

aauaaacugg uauuugaaaa gaaaaucaua cgcgucacua uuauggagcg gaaggccuuc     3600

gaaaaggaug agaaagcauu uuuggaggaa cagggcuaua gacagccgaa ggugcuggcc     3660

aaauuaccca aguacacccu cuaugagugu gaagagggga ggcgacggau gcuggcuuca     3720

gcgaacgaag cccagaaagg aaaucaacag gugcuuccua accaucuggu cacguugcuc     3780

caccaugcag cuaacugcga gguuagugac ggcaagagcc uggauuacau cgagucgaau     3840

cgcgaaaugu ucgccgagcu acuggcccac guguccgaau uugcuaagag auauacccuu     3900

gcagaggcca accucaauaa aaucaaccag cuguucgaac agaauaagga gggugacauu     3960

aaagcgauag cucaaucuuu cguagauuug auggccuuua acgcaauggg ggccccagcu     4020

agcuucaagu uuuucgagac aacuaucgaa aggaaacggu acaauaaccu gaaggaguua     4080

cucaauucaa ccauuaucua ccagaguauu acaggacugu augaaucccg aaagagacuu     4140

gacgauaaac gccccgcagc cacuaagaaa gcgggccagg cuaagaaaaa gaaauaagga     4200

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4260

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aagaauu                                                    4337


<210>  3546
<211>  4370
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3546
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaag aaaccauaca gcauuggacu ggacauaggu      180

accaacuccg uuggcugggc cgugguaaca gaugacuaua aggugccggc gaagaaaaug      240

aagguccucg ggaauacuga uaaaucucau aucgagaaga accuucuggg agcuuuguua      300

uucgacucag gcaauacggc cgaagauagg cggcugaaac gcaccgcacg acguagauac      360

acaaggcggc gcaaccggau ucuauaucuc caggagaucu uuagugaaga gauggguaag      420

guggacgaua gcuucuuuca caggcuggaa gacuccuucc uuguuacuga ggauaagaga      480

ggggagcgcc aucccauuuu uggaaaucug gaagaggaag ugaaauacca cgagaacuuc      540

ccuaccaucu aucauuugcg acaauaccuc gccgacaauc cagagaaggu cgaucugaga      600

cugguguauc ucgcuuuagc acacauaauc aaauuuaggg gccacuuccu gauugaaggc      660

aaguuugaca cacggaacaa ugauguacag cgucuuuucc aggaguuuuu ggccguuuac      720

gacaacaccu ucgaaaauuc uucgcugcag gagcaaaacg ugcaggucga agagauccua      780

acugacaaaa uuucaaagag cgcuaaaaag gaucgcgugc ucaagcuguu ucccaaugag      840

aaaaguaacg ggagauucgc cgaguuccuu aagcugaucg ugggaaauca ggcggacuuu      900

aaaaagcauu ucgaguugga agagaaagca ccucuccagu uuuccaagga uacauacgaa      960

gaggagcugg aagucuuacu ggcccaaaua ggugacaacu augcugaacu cuuccuuucu     1020

gccaagaaac uguacgauag cauuuugcua uccggcaucc ugaccguuac ggacgugggg     1080

acuaaggcac cacucucagc uaguaugauu cagagguaua acgaacacca gauggaucug     1140

gcccagcuua aacaauuuau ccggcagaag cugagcgaca aauacaauga gguauucucu     1200

gaugugucca aggacggaua ugcgggcuac aucgauggua agaccaacca ggaagccuuu     1260

uauaaauacu ugaaggggcu ccugaauaaa auugagggau caggcuacuu cuuagacaag     1320

auagagcgag aagacuuucu gcgcaaacaa agaacauucg auaacggcag caucccccau     1380

cagauucacc uacaggagau gagggcaauc auacggcgcc aggcugaauu uuauccuuuc     1440

cucgccgaca aucaagauag aauugagaag cugcuuacuu uuaggauccc auacuauguc     1500

gggccguugg cucguggaaa gagugacuuc gccuggcugu cucggaaauc cgcagauaag     1560

aucacccccu ggaacuucga cgagauugug gauaaagaaa gcucggccga ggcuuuuauc     1620

aaucgaauga caaacuacga ccucuaucug ccuaaucaga agguucuucc aaaacacucu     1680

cuguuguacg aaaaguucac cguguauaac gagcucacga aagucaagua caagacugaa     1740

caggguaaaa cagcguuuuu cgaugcaaau augaagcagg agauuuuuga cggcguguuc     1800

aaaguauaca gaaagguuac caaagauaag cugauggacu uuuuagagaa ggaguucgau     1860

gaauuuagga uaguggaccu gacugggcua gacaaagaaa acaaggucuu caaugccucc     1920

uauggaacau accaugaucu cugcaaaauc cuggacaagg auuuucuuga caacaguaaa     1980

aaugagaaga uuuuggaaga uaucgugcug acccucacgc uguucgagga ccgcgagaug     2040

auccggaaga gauuagaaaa cuauucagau cugcuuacca aagagcaagu caagaaauug     2100

gaaaggcgcc acuacacagg cuggggucga cucagcgcug agcugauuca ugggauacgg     2160

aacaaggaau cccguaaaac uauccuagac uaucugauug augacggcaa uucuaacaga     2220

aauuuuaugc agcucaucaa cgaugacgcc cugagcuuca aggaggagau ugcaaaggcc     2280

caggugaucg gagaaaccga uaaucuuaac cagguugugu cagacauagc uggcaguccc     2340

gccaucaaaa aggggauuuu gcaaucccug aaaaucguag acgaguuagu caagauuaug     2400

ggacaccagc cugaaaauau cgugguugag auggcaaggg agaaccaguu cacaaaucag     2460

ggucgccgga acagccaaca gagacucaaa ggccugacug auucuauaaa ggaauuuggg     2520

ucacagauuc uuaaagagca uccaguggaa aauagucaac ugcagaacga cagguuguuc     2580

cucuacuauc ugcagaaugg acgagauaug uacaccggcg aggaacuaga caucgauuac     2640

cugagccagu augacauuga ucacaucaua ccccaagcgu uuaucaagga caacuccauu     2700

gauaaucgcg uccucacauc uucgaaggag aaccggggua aaagcgacga ugugccgucc     2760

aaggacguag ugagaaaaau gaagucauac uggaguaaac ugcuuucugc uaaguugauc     2820

acccagagga aguucgacaa ccugacuaaa gccgagcgug ggggcuuaac ggaugacgau     2880

aaggcaggau uuauuaaacg ccagcucguu gaaacacggc agaucaccaa gcacgucgcc     2940

agaauacugg acgagagguu caauacugaa accgaugaga acaauaaaaa gauucgacaa     3000

gugaagaucg ugacacuuaa aagcaaccug gucuccaauu uucggaagga guucgaacua     3060

uauaaaguuc gcgagaucaa cgacuaccau cacgcucaug augccuaucu caaugcagug     3120

auuggcaagg cucuguuggg gguguacccu cagcuggaac ccgaguuugu auauggagac     3180

uacccacacu uccacgguca uaaagaaaac aaggccaccg cgaagaaauu uuucuacuca     3240

aauaucauga acuucuuuaa gaaagaugac gucagaacug auaagaaugg cgagauuaua     3300

uggaaaaagg acgaacacau cagcaacauu aagaaagugu uaucuuaucc ucagguuaau     3360

aucgugaaga aagucgagga gcagacaggg ggauucucca aggaaaguau ucuccccaaa     3420

ggcaacagcg auaagcugau cccaaggaaa acgaagaagu uuuacuggga caccaaaaag     3480

uauggugggu ucgacucucc cauaguggcc uacuccaucc uuguuauugc ugauaucgag     3540

aaaggcaaga guaaaaagcu gaagacugug aaagcauugg uaggagucac aauuauggaa     3600

aagaugaccu uugagcggga cccuguggcc uuccucgagc gaaaaggcua uagaaaugug     3660

caagaagaga acaucauaaa gcugccaaaa uacucacuau uuaagcugga aaacgggcgc     3720

aagaggcuuc ucgcuagcgc ccgugagcug cagaaaggaa augaaauugu cuugccgaac     3780

caucugggca cauuacucua ucacgcaaag aauauccaua aaguugauga gcccaagcac     3840

cuggacuacg uggauaaaca caaggacgag uucaaggaac uucuggaugu agugucgaac     3900

uuuuccaaaa aguacacuuu ggcggagggu aaucucgaga aaaucaagga acuguaugcc     3960

cagaacaaug gggaagaccu aaaagagcug gcuucuagcu ucauuaaccu ucucaccuuu     4020

acggccauag gagcuccugc aaccuucaag uuuuucgaua aaaauaucga ccggaagaga     4080

uacacaucaa cuaccgagau ucugaacgcc acauugaucc aucaaaguau uacuggcuua     4140

uaugaaacca ggaucgaucu gaauaagcuc gguggcgaca aacgcccagc agccacgaag     4200

aaagcugggc aggcgaagaa aaagaaguaa ggacuagucc cuguucccag agcccacuuu     4260

uuuuucuuuu uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                4370


<210>  3547
<211>  3503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3547
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcacag acaacaaacc ugagcuacau ucucggacuu      180

gaccugggua uagccuccgu uggcugggcg gugguagaga ucaaugaaaa cgaggaucca      240

auuggguuga ucgacguggg agucaggauu uucgaacggg cugaggugcc gaagacuggc      300

gaaucuuuag cccugucacg ccgacuagca cguaguacga gaaggcucau ccggcgcaga      360

gcccauaggc ugcuucuggc uaaacgguuu uugaagcgcg aggguauacu cagcaccauc      420

gaucuggaga aggggcugcc caaucaagca ugggaacucc gaguugccgg auuagagaga      480

aggcuguccg cuauugaaug gggcgccgug cuuuugcacc ugaucaaaca ucggggcuau      540

cuaucuaagc guaaaaacga gucgcagaca aauaacaagg agcucggggc gcugcuuuca      600

ggagucgcac agaaucacca gcuguugcaa agcgacgauu accgcacucc ugccgaacuc      660

gcucugaaaa aguucgccaa ggaggaaggu cauauuagaa accagagggg cgcauauacc      720

cacacauuua aucgguuaga ccugcucgcu gagcuuaacc uguuguucgc ccagcagcac      780

caauuuggga auccacauug caaagaacac auccagcagu acaugaccga gcuacugaug      840

uggcagaagc ccgcgcucag uggagaggcc auacugaaaa ugcuuggcaa guguacucau      900

gagaaaaacg aguucaaggc agcuaaacac acauauuccg ccgaacgauu uguguggcug      960

accaaguuga auaaccuccg cauucuggag gauggugcug aaagagccuu aaaugaggag     1020

gaaaggcaac ugcuaaucaa ccacccuuac gagaagucua aacucacgua ugcacaggua     1080

cggaagcugc uuggguugag cgaacaggcc auuuucaaac aucugcgcua cuccaaggag     1140

aacgcugaau cagcgacuuu uauggagcuc aaagcauggc acgccaucag aaaggcucug     1200

gagaaucaag gacuuaagga caccuggcag gaucuggcca aaaagccaga cuugcucgau     1260

gaaaucggca cagcauucag ucuguacaaa acugacgagg auauucagca guauuuaacc     1320

aacaagguuc ccaauagcgu gauaaacgcc cugcuagucu cucucaauuu ugacaaauuc     1380

aucgaacugu cccuuaaguc auugaggaag auucugccuc ucauggagca aggcaaacgu     1440

uacgaccagg cuugccggga gaucuauggg caucacuacg gagaagccaa ccagaagaca     1500

agccagcugu uaccagcaau accggcgcaa gagauucgaa aucccguggu gcugagaacc     1560

cuuagucagg cuaggaaagu caucaacgcc aucauucgcc aguaugguuc uccugcacgg     1620

guucauaucg aaacgggcag agaguugggg aaguccuuua aagaaaggcg cgagauucag     1680

aagcaacagg aggauaaucg aacuaagcgg gaaagcgccg ugcagaaauu caaggaacuc     1740

uucucggacu uuucuuccga accaaaauca aaggauauac ugaaauuccg ucuauacgag     1800

cagcaacacg gaaagugucu guauaguggc aaagaaauca acauucacag acucaaugag     1860

aaggguuacg uagagaucga ccaugcucug cccuuuagca ggacauggga ugacuccuuc     1920

aacaauaagg ugcuugucuu ggccucugaa aaccagaaua aagggaacca gaccccuuac     1980

gaguggcugc aaggcaagau caacagcgaa cgcuggaaaa auuuuguggc auuaguucuc     2040

ggaucacagu gcagugcugc caagaaacag cggcugcuua cucaggugau ugaugacaac     2100

aaguucauag auagaaaucu gaacgacaca agguauaucg cgcgauuuuu guccaauuac     2160

auucaagaga accuccugcu agucggcaag aauaaaaaga acguguucac cccaaauggg     2220

cagaucacgg cccugcuccg cagccggugg ggacugauua aagcuagaga aaacaauaac     2280

aggcaccaug cacuugaugc caucguaguu gcuugugcca cccccucuau gcagcagaag     2340

auaacacguu uuauccgcuu caaagaggug cacccguaua agauugagaa ucgguacgaa     2400

auggucgacc aagagucagg ugaaaucauu aguccucacu uucccgagcc augggcauau     2460

uucagacagg aggugaacau cagggucuuu gacaaucauc cugauacugu guugaaggaa     2520

augcugcccg accgaccaca ggcgaaccac caguucguuc aacccuuauu cgugagccgg     2580

gccccuaccc gcaaaauguc cggccagggg cauauggaga caauaaaguc ugcuaaaaga     2640

cucgccgaag gaauuucggu acugaggauc ccacuuacuc agcugaagcc gaaccuacuc     2700

gagaauaugg ucaacaaaga acgggagccc gcacuguacg cuggcuugaa ggcccgacug     2760

gcagaguuua aucaagaucc ugccaaggcu uucgcgaccc cauuuuauaa acaggguggg     2820

caacagguga aggccauuag aguugaacag gugcagaaaa gcggcgucuu agugcgcgag     2880

aacaauggag uagcagacaa cgcuuccauc gugaggacag auguuuucau aaagaauaac     2940

aaauuuuucc ucguccccau cuacaccugg caaguggcca agggcauucu gccuaauaaa     3000

gccaucgugg cucacaagaa cgaagacgag ugggaagaga uggaugaggg ggcaaaguuu     3060

aaauucucac uuuuuccaaa ugaccugguc gaauugaaga cuaaaaagga guacuucuuu     3120

ggauauuaca uuggucucga ucgugccacg ggcaacauca gucugaaaga acaugacggg     3180

gagauaucua agggaaagga uggcguuuau cgggugggug ugaaacuagc gcugagcuuc     3240

gaaaaguacc agguagacga gcuugggaaa aauagacaga uuugcaggcc ccagcagcgc     3300

cagccggucc gaaagagacc ugcugccacc aaaaaggcag gccaagccaa gaaaaagaaa     3360

uaaggacuag ucccuguucc cagagcccac uuuuuuuucu uuuuuugaaa uaaaauagcc     3420

ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaga auu                                             3503


<210>  3548
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3548
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcagcc gcguucaagc caaacccgau uaauuacaua      180

cugggacucg acaucgguau ugcuagcguu ggcugggcca ugguggagau cgaugaagac      240

gagaacccca uuugccuuau cgaucugggg guaagggugu uugaacgggc agaggucccu      300

aaaaccggag acuccuuggc cauggcucgc cgauuagcac guucugugag aaggcugaca      360

cggcgcagag cccauaggcu acuccgggcu cgccgacugc uuaagagaga aggcguucug      420

caggccgcgg auuucgacga gaaugguuug auaaagucac ucccaaacac ucccuggcaa      480

cugagggcag ccgcucugga ucggaaacuc acgccuuuag aguggagugc cgugcugcuu      540

cacuugauca agcaucgugg guaucugagc cagcgcaaaa augaaggaga gaccgcagac      600

aaggaacuag gcgcucuccu gaaaggcguc gccgauaacg cgcacgcccu ucagacaggg      660

gacuuuagaa cuccagcaga gcuggcuuug aauaaguucg agaaggaauc cggacauauu      720

aggaaccagc ggggugauua cucucacacc uuuucgcgaa aagaccucca agccgagcug      780

aucuuacugu ucgaaaagca gaaagaguuu ggcaaucccc acgugucagg gggacucaag      840

gaaggcauug agacacuucu gaugacccag cgcccugcuu ugagcgguga ugccguacag      900

aaaaugcuag ggcauuguac uuucgagcca gcagaaccga aggccgcuaa aaacacauau      960

accgcggaga gauuuaucug gcugacgaag cucaauaacc ugaggauacu ugaacaagga     1020

agugagcggc cccugacuga caccgaacgc gcaacauuga uggacgagcc uuacagaaag     1080

uccaaacuca cuuaugccca ggcuaggaag cuguuaggcc uggaggauac cgccuucuuu     1140

aaaggccuac guuacgggaa ggacaaugca gaagccucua cacucaugga gaugaaggcu     1200

uaucacgcca uuagccgggc acuggaaaag gagggacuua aggauaaaaa guccccauug     1260

aaccugucac ccgaacucca ggacgagauc gguaccgcgu ucagucuguu uaaaacggau     1320

gaggacauua cuggccgacu uaaggauaga auccagccug aaauccugga ggcuuugcuc     1380

aaacauauua gcuucgacaa guucguucaa auaucucuga aggccuuaag gcgcaucgug     1440

ccacugaugg aacaggggaa acgguacgau gaggcaugcg ccgagauuua cggagaccac     1500

uauggcaaga aaaauacaga agagaagauc uaccuacccc cgauaccugc ugaugaaauu     1560

agaaaccccg ucgugcucag ggcccugucc caggcacgca aagugaucaa cggugucguu     1620

cgacgguaug ggucaccagc ucguauccac auugagaccg ccagagaagu gggcaagagc     1680

uuuaaggaca ggaaagagau cgagaagcgc caagaagaga aucggaaaga cagagaaaag     1740

gcggccgcua aauucaggga guacuuuccu aacuucguag gagaacccaa gaguaaagau     1800

auucuuaagu ugcgacugua ugagcagcag cauggcaagu gucucuacuc ugggaaagag     1860

auaaaucugg gacgcuuaaa cgaaaagggu uauguggaga ucgaccacgc acugccauuu     1920

ucccggacuu gggaugacag cuucaauaac aaaguccuug uguugggcuc ggaaaaucag     1980

aacaagggga aucaaacacc cuacgaguac uuuaacggaa aagauaauuc uagagaaugg     2040

caggaguuca aggccagggu ugagaccucc cguuuuccuc gcucaaagaa acagcggauu     2100

cuccugcaga aguucgacga agauggcuuu aaagagagaa accuaaauga cacgagguau     2160

gugaaccgau uccugugcca auuugucgcu gaucggaugc gccucaccgg uaaggggaaa     2220

aagagagugu ucgccaguaa uggccagauc acaaaccugc uuaggggauu cuggggcuug     2280

cggaagguac gagcagaaaa cgacagacau cacgcgcugg augccguugu ggucgcuugu     2340

agcacugugg ccaugcagca gaaaaucacc cgcuuuguca gguacaagga gaugaaugca     2400

uucgacggga aaacaauuga uaaggagacu ggagaagugu uacaucagaa aacccacuuu     2460

ccacagccgu gggaguucuu ugcucaggaa guuaugauac guguguucgg uaagcccgac     2520

ggcaagccug aguuugaaga ggccgacaca ccagagaaac uccggacccu gcuugcagaa     2580

aagcuguccu cuagacccga ggccguacac gaauauguca cuccuuuguu cgugagcagg     2640

gcuccaaacc gcaaaauguc agggcaggga cauauggaga cgguuaagag ugcgaaacga     2700

cucgaugaag gcguguccgu ccugagagug ccccuaacac aacugaagcu caaagaccug     2760

gagaagaugg uaaaucggga gagggaaccg aaacuuuacg aggccuugaa agcacgccug     2820

gaagcucaca aggaugaccc ugccaaagcc uuugcugagc cauucuauaa guacgauaaa     2880

gcagguaacc guacccaaca ggugaaggcc guuagagucg aacaagugca gaagacuggg     2940

gugugggucc ggaaucauaa cggcaucgcg gacaaugcua ccaugguuag gguggaugug     3000

uuugagaaag gagacaagua uuacuuagua cccauuuaca gcuggcaggu cgccaaaggc     3060

auccucccug aucgcgcagu gguucagggg aaggacgagg aagauuggca acugauugac     3120

gauucuuuca acuuuaaauu cucacuucac cccaaugacc ugguggaggu caucacaaag     3180

aaggcccgaa uguucggaua uuuugcuagu ugccacagag gcaccgguaa cauaaauauc     3240

cggauucaug accuagauca caaaaucggg aagaacggca uucucgaagg aaucggugug     3300

aaaacugcac ugagcuucca gaaguaccag auagacgagu ugggcaaaga gauuaggcca     3360

ugucgccuga agaagagacc ucccguuagg aaacggccag ccgcgacaaa gaaagcuggg     3420

caggccaaga aaaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3480

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  3549
<211>  3587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3549
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cacccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaag uaccacguug gaauugacgu ggguaccuuc      180

agcguaggcc uggccgcgau agagguggau gacgcuggga ugccaaucaa aacacucucc      240

cuugucucuc acauucauga uucaggacug gacccggaug aaaucaagag ugccgugacu      300

agguuggcaa gcuccggcau ugcccggcgc acgcgacguu uauauagaag gaagcggcgc      360

agacugcagc aacuagacaa auuuauccag aggcaggguu ggcccguuau agagcucgaa      420

gauuacucug acccucugua uccauggaag gugcgggcug agcuugcagc cucguacauc      480

gcugaugaaa aagagcgcgg ggagaagcug ucagucgccu ugcgacacau ugcgagacau      540

aggggauggc ggaaccccua ugcaaaagug agcagucucu accugccuga cggcccaucc      600

gaugccuuca aggcuauccg ugaagagauu aagcgcgccu cuggccagcc cguaccugaa      660

accgcaacag uugggcaaau ggugacucug ugcgagcucg gaaccuuaaa acugagaggu      720

gagggcgggg uccuuagcgc uagguugcag caguccgacu augcccggga aauccaggag      780

auaugucgaa ugcaagaaau uggacaggag cuguaccgca agaucauuga ugugguguuu      840

gcggccgaau caccaaaagg cagugcaagc ucuagagucg guaaggaccc gcuacagccc      900

gggaaaaaua gggcucucaa ggccuccgac gcuuuccagc gguaccgcau cgccgcacug      960

aucggaaacc uuagaguuag gguggauggc gagaaacgua uucugucagu agaggaaaag     1020

aauuuggugu uugaccaccu cgucaaccug acaccuaaga aagagccaga augggugacc     1080

auagccgaga ucuuaggcau ugaucggggg caacugaucg gaacugcuac aaugaccgac     1140

gauggugaac gagcgggcgc aagacccccu acgcacgaca cuaauaggag cauaguuaac     1200

agucgcauug ccccacucgu ggauuggugg aagaccgcuu cugcccuuga gcagcaugca     1260

auggucaaag cccuguccaa ugcugaggug gacgauuucg acagccccga aggggccaag     1320

guacaggcau uuuucgcgga uuuggacgac gauguucacg cuaaacuaga cucgcugcau     1380

cucccggugg gacgggccgc auauucugag gauacacugg ucagacuuac uaggcgcaug     1440

cuguccgacg gcguggauuu guacaccgcc cgacuccaag aauuugguau cgagccuuca     1500

uggacacccc caaccccucg gaucggggaa cccgucggca acccagcugu ggaccguguu     1560

cugaagacgg ugaguagaug guuagagagc gccacuaaga cauggggagc acccgagagg     1620

guaauuaucg aacacguccg cgagggcuuc gugaccgaaa aacgggcuag agagauggau     1680

ggggacauga ggcgacgcgc cgcgcggaau gccaagcugu uucaggagau gcaggaaaaa     1740

cuaaacguuc agggaaagcc uuccagagcu gaucucugga gguaucaauc ugugcagcgu     1800

cagaauugcc agugugcaua cugcgguagc ccaauuacuu ucucaaacag ugagauggac     1860

cacauagucc cgcgcgccgg ccaagggucc acaaauaccc gggaaaaccu gguggcugua     1920

ugucauagau gcaaucagag caaaggaaac acgcccuuug ccaucugggc aaagaacacc     1980

ucuauugagg gcgugucagu uaaggaagcg gucgagagga cacgacacug ggugacugau     2040

accgguaugc ggaguacaga cuucaaaaag uucacuaaag ccguggucga gcgcuuucag     2100

agagcuacca uggaugaaga gaucgacgcc aggagcaugg aauccguugc auggauggcu     2160

aaugagcuuc ggucucgagu ggcccagcau uucgcaucgc acgggacaac cgugagagua     2220

uaucgcggca gcuugacugc cgaagcuagg cgugcguccg gaaucucagg caagcugaaa     2280

uuuuucgacg gggucggaaa gagucggcuc gauagaaggc aucacgccau ugacgcagcu     2340

gugauagccu uuacgucuga uuacguugcc gagacacugg cugugcgcag caaccuuaag     2400

caaucccagg cacaccgaca ggaggccccu caguggagag aguucaccgg uaaagacgcg     2460

gaacaucggg cugccuggag ggucuggugu caaaagaugg aaaaacuguc agcauugcuc     2520

acugaggauc ugcgcgacga ucgugugguu gugaugagca auguaagauu acggcugggc     2580

aacgggucug cccacaagga aaccaucgga aaacuaucca aggucaaacu caguagccag     2640

cugucugugu ccgacauuga uaaggcuuca agugaggcac uuuggugcgc cuugacaagg     2700

gagccaggcu uugaccccaa ggaaggucug ccugcgaauc cagagcgcca uauccgagug     2760

aacgggaccc acgucuaugc uggcgauaau auuggacucu uccccguuag cgccggcucg     2820

aucgcacuga gagggggaua cgccgaauua ggcuccucuu uucaccaugc ucggguguac     2880

aaaauaacua gcgguaagaa accggccuuc gcaaugcuga ggguauauac aaucgaccuu     2940

uugccuuacc gcaaccagga ucucuuuuca guggagcuga agccacaaac gaugaguaug     3000

agacaggcug agaaaaagcu aagggacgcc cuggcgaccg ggaaugccga auaucucgga     3060

uggcuggucg uggacgauga gcuuguugug gacacuucca agauugcaac agaucagguc     3120

aaagcugugg aagccgaguu gggcaccauc cggcguuggc gcguugacgg uuucuuuucu     3180

cccagcaagc ugcgauuaag accucuccag auguccaaag aaggcauuaa gaaagaguca     3240

gcucccgagc ugagcaagau cauagauagg ccaggguggc uuccugcagu gaacaagcug     3300

uucagugacg gaaaugucac aguagugcgg cgcgauucuu ugggcagagu uaggcucgaa     3360

uccacugccc aucugcccgu gaccuggaaa guccaaaagc ggccagccgc uacgaaaaag     3420

gcggggcagg ccaaaaagaa gaaauaagga cuagucccug uucccagagc ccacuuuuuu     3480

uucuuuuuuu gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   3587


<210>  3550
<211>  3287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3550
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcagcc aggauucugg cguucgacau aggaaucagc      180

uccauugguu gggcuuuuuc ugagaacgau gaacucaagg acugcggcgu ucggaucuuc      240

accaaagugg agaauccaaa gacaggggaa ucacuugccc ugccgcgccg auuggcacgu      300

agugccagaa agagguuagc ucggcgcaaa gcaagacuga accaucuaaa gcaccucauu      360

gccaaugagu uuaaacugaa cuacgaagau uaucagagcu ucgacgaguc ccuugcuaag      420

gccuacaaag gaucucugau cucgcccuau gaguugaggu uucgggcgcu caaugaacug      480

cugucaaagc aagauuucgc acgcguaaua cuccauaucg ccaagcgaag aggcuacgac      540

gauauuaaaa acagcgacga uaaggagaaa ggugcuaucu uaaaggccau uaaacagaau      600

gaagagaagc uggcaaacua ucagagugug ggggaguacc uuuauaaaga auacuuucag      660

aaguucaagg aaaauuccaa agaauuuacu aacgucagga auaagaaaga gucuuacgaa      720

cgguguaucg cucagagcuu cuugaaggac gagcugaaac uaauauuuaa gaagcaacgu      780

gaguucggau uuuccuucuc uaaaaaguuc gaagaggaag ugcucagugu ugccuuuuau      840

aaacgcgcgc ugaaggauuu cagccaccuu gugggcaacu gcucauuuuu cacggacgag      900

aaaagagccc cuaaaaauuc cccacuggca uuuauguucg ucgcuuugac caggauuauc      960

aaccuccuga auaacuuaaa gaacacagaa ggcauucugu acacuaaaga ugaccucaau     1020

gcccuucuga acgagguguu gaagaauggg acccuaacau auaaacagac caagaaacug     1080

cucggacugu cagacgauua cgaguuuaag ggugaaaagg gcacuuauuu caucgaguuu     1140

aaaaaguaca aagaguucau caaggcucuu ggggagcaua accugagcca ggacgauuug     1200

aaugaaauug ccaaagacau aacacucauc aaggaugaga uuaaacugaa gaaggcauua     1260

gccaaauaug accugaacca aaaucagauc gauagucuau cuaagcucga guuuaaagac     1320

caccugaaca uauccuucaa ggcucuuaaa uugguaacuc cccugaugcu cgaaggaaag     1380

aaguacgaug aggcguguaa ugaacugaac cuuaaaguug caauuaauga ggacaagaaa     1440

gauuuucugc cugccuucaa cgagacguac uauaaggacg aagugacuaa uccagucgug     1500

uugcgggcua ucaaagagua ccgaaaggug cucaacgccc uguuaaagaa auauggcaag     1560

guccacaaaa ucaauauuga acuggcacgc gagguuggua agaaccauag ccagagagcc     1620

aaaaucgaaa aggagcagaa cgagaauuac aaggcuaaaa aggacgccga acuagagugc     1680

gaaaaacucg ggcugaagau uaacucgaaa aauauacuua aguugaggcu guucaaagag     1740

caaaaggaau uuugugcaua uucuggagag aagaucaaaa uuuccgaucu ccaggacgag     1800

aagaugcugg aaaucgauca caucuacccc uauucacgga guuucgacga uagcuacaug     1860

aacaaagugu uaguauuuac caagcagaau caagagaaac ugaaccagac accuuucgaa     1920

gcguuuggca augacuccgc uaaguggcag aagauugagg ugcuugccaa aaacuugcca     1980

acuaagaaac agaagcgcau acucgauaaa aauuacaagg acaaggaaca aaagaacuuc     2040

aaggauagaa aucugaacga caccagguau aucgcacguc uaguccugaa uuacacaaaa     2100

gauuaucucg acuuucugcc gcuuucugau gacgagaaca ccaaguugaa ugacacgcag     2160

aaaggcagca aggugcaugu ugaggccaag ucagggaugc ugaccagugc uuuacggcac     2220

acauggggau ucuccgccaa agaucgaaac aaccaccucc aucacgcaau ugacgcugug     2280

aucauugccu acgcgaauaa cagcaucguc aaggccuuuu cugauuucaa aaaggaacag     2340

gagucaaaua gugcugaacu guaugccaaa aagauaagcg agcuugacua caagaacaaa     2400

agaaaguuuu ucgagcccuu uuccgguuuc aggcagaaag ugcuggauaa gaucgacgaa     2460

auuuucguau cuaaaccuga gcgcaagaaa ccaucgggcg cauugcauga agagaccuuu     2520

cggaaggaag aggaguucua ucaaagcuac gggggaaagg aaggcguucu caaagcucug     2580

gagcuaggua agaucagaaa agugaauggg aagauuguca aaaacggcga uauguuuagg     2640

guggacaucu ucaagcacaa gaaaacuaau aaguuuuacg ccguccccau auauacaaug     2700

gauuucgcac ugaaagugcu cccuaacaag gcgguugccc gcuccaaaaa gggagaaauu     2760

aaggacugga uccugaugga ugagaauuac gaauuuugcu ucucacuuua uaaagacagu     2820

uugauucuga uccagaccaa ggauaugcag gagccagagu uuguguacua uaacgcuuuc     2880

acgucuagca ccguauccuu aauagucuca aaacaugaca auaaguuuga aacacucagc     2940

aaaaaccaga agauccuguu caagaaugcc aacgagaaag aagugauugc aaagucuauc     3000

ggcauucaaa aucuuaaagu uuucgagaag uacaucgugu ccgcucuggg ggaagucacu     3060

aaagccgagu uucgacagcg ggaggacuuc aagaaaaagc gucccgcagc caccaagaaa     3120

gcuggacagg cgaagaaaaa gaaauaagga cuagucccug uucccagagc ccacuuuuuu     3180

uucuuuuuuu gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   3287


<210>  3551
<211>  3698
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3551
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaagc gaccugguuc ucggacuuga uauugguaua      180

ggcuccgugg ggguaggaau ccugaacaag gugaccggcg agauuaucca uaaaaauucu      240

aggauuuucc cagccgcgca ggcugaaaac aauuuggucc ggcgcacaaa ccgacaaggu      300

cguagauuag ccaggcggaa gaagcaccgc agagugaggc ugaaucggcu auuugaggaa      360

ucagggcuca ucacugacuu cacgaaaaua aguaucaacc ugaauccgua ccagcuucgc      420

guuaagggac ugaccgauga guugagcaac gaagaacucu uuauugcacu gaaaaauaug      480

gugaagcauc gaggcaucuc cuaucuggac gaugccucug acgauggcaa cucgucaguc      540

ggggacuacg cucagauugu gaaagagaau agcaagcagc ucgaaacaaa gacucccgga      600

caaauccagu uagagagaua ucagaccuac ggucagcuga ggggcgauuu cacaguagaa      660

aaagacggga agaaacaccg gcuuauaaac guuuuuccua ccagugcaua ucguuccgag      720

gccuugcgca uucugcaaac ucagcaggag uucaauccac agaucacaga ugaauuuauu      780

aacagauacc uagagauccu caccggaaag aggaaauauu accauggccc cgguaaugaa      840

aagucucgga cggacuacgg gcgauaucgc acuagcggag agacccugga uaacaucuuc      900

ggcauucuua uaggcaaaug cacauuuuac ccugacgagu ucagagcugc caaggcgucc      960

uauacugcac aagaguuuaa ccuguugaau gaccucaaca aucugaccgu gccaacagag     1020

accaagaaau uaucaaagga acagaaaaac cagaucauua auuacgucaa gaacgagaaa     1080

gccauggggc ccgcuaagcu guucaaguau aucgccaaac uccuuaguug ugauguggca     1140

gacauaaagg gauacaggau ugauaaaagc gguaaggcug aaauccacac guuugaggcc     1200

uaucggaaaa ugaagacucu ggaaacauug gacaucgagc aaauggaucg cgagacccua     1260

gacaagcugg cguacgugcu cacucugaau acagaaagag agggcauuca ggaagcccuu     1320

gagcacgagu ucgcagaugg gucuuucucc cagaaacagg ucgacgagcu gguucaauuu     1380

aggaaggcua acucaagcau cuucggaaaa ggcuggcaua auuuuagugu gaaguugaug     1440

auggagcuca uuccugaacu guacgagacc ucugaagaac agaugacgau auuaacccgu     1500

cuggguaaac agaagacaac uuccagcucg aacaagacca aauauaucga ugagaagcua     1560

cucacagaag agauuuacaa uccaguagug gccaaaucug uccggcaggc uaucaagauc     1620

gugaacgccg caauuaaaga auauggggac uucgauaaua uaguuaucga gauggcccga     1680

gaaacuaacg aggacgacga gaagaaagcu auucaaaaga uccagaaggc gaauaaagau     1740

gaaaaggacg cagccaugcu gaaagcugcc aaccaguaca acggcaaggc agagcuuccg     1800

cacuccgugu uucauggaca caaacaguug gccaccaaga uuagacugug gcaccaacag     1860

ggcgaaaggu gccucuauac agggaagacc aucucaauac augaucugau caauaacagu     1920

aaucaguucg aggucgacca cauucuuccc cugagcauca cuuuugauga cuccuuggcu     1980

aacaaagugc ucguauacgc cacggcaaau caggaaaagg gacaacgcac accuuaucag     2040

gcgcuggauu cuauggacga ugcuuggagc uuccgggagu uaaaagccuu uguuagagag     2100

ucaaagaccc ugaguaacaa aaagaaggaa uaccuacuca cugaggaaga cauuuccaaa     2160

uucgauguga ggaagaaauu uaucgagcgc aaucuggucg acacccgaua cgcaagccgg     2220

gugguccuua acgccuugca ggaacauuuc cgugcucaca agauagauac aaaagugucu     2280

guugugagag gucaauuuac cucacagcug aggcgccauu ggggcauuga gaagacucgg     2340

gacacauauc accaccaugc cguagacgca cucaucauug cugccaguag ccagcugaau     2400

uuauggaaga aacagaagaa cacgcugguc uccuacucug aggaucaacu uuuggacauc     2460

gaaaccgggg agcucauauc ggaugacgaa uauaaagaga gcguguucaa ggcgccauac     2520

cagcacuucg uugauacucu gaaauccaag gaguuugaag acucaauccu auucaguuau     2580

cagguggauu cuaaauuuaa uagaaagauu agcgacgcca caaucuacgc uaccaggcag     2640

gcaaaggucg gaaaagauaa ggccgacgag acauaugugc ugggcaaaau uaaggauauc     2700

uacacucaag acgguuacga ugcuuucaug aaaauauaua agaaggacaa auccaaguuu     2760

cucauguacc gacaugaccc ccagaccuuc gaaaaaguaa uugagccuau ccuggaaaac     2820

uauccaaaua agcagaucaa cgagaaaggg aaggaggugc ccuguaaucc guuucuuaag     2880

uacaaagagg aacacggcua uauucgcaag uacagcaaaa agggaaacgg cccugaaauc     2940

aaaucauuga aguauuacga uucuaagcug gggaaccaca uugacauaac gcccaaagau     3000

uccaauaaca agguugucuu acagagugug agcccauggc gggccgacgu guacuucaau     3060

aaaaccacag gaaaguauga gauccucggu cugaaauacg cagaucuuca auuugaaaag     3120

ggcacuggga ccuauaagau uucucaggag aaauacaacg acaucaagaa aaaggaggga     3180

gucgauuccg acucagaguu caaauuuaca cuguauaaga augauuugcu ccugguuaaa     3240

gacacugaaa ccaaggaaca gcaacuauuc agauuucuga guaggacgau gccuaagcag     3300

aaacauuacg uggagcucaa gcccuaugau aaacagaagu ucgaaggcgg ugaggcgcug     3360

auuaaagugc uugggaacgu agccaauagc ggccagugca agaagggauu gggcaaaucg     3420

aacaucucca uauacaaggu ccguacagac gugcugggga aucaacacau cauuaaaaac     3480

gagggagaca agccaaaauu agauuucaag cgccccgcug ccaccaagaa agcaggccag     3540

gcuaagaaaa agaaauaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu     3600

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                             3698


<210>  3552
<211>  4256
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3552
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaacc caguucgagg gauuuacaaa ccuguaccaa      180

guuagcaaga cucucagguu cgaacuuauu ccacagggua aaacgcugaa gcauauacag      240

gagcagggcu uuaucgaaga ggacaaggcc cggaaugauc acuauaaaga auugaagccg      300

auuaucgacc gcauuuacaa aaccuaugcg gaucaaugcu uacagcuggu gcagcuagac      360

ugggagaacc ucuccgcugc caucgauucu uaccgaaagg agaaaacaga agagacucgu      420

aaugcacuga uagaggaaca ggccaccuau agaaacgcua uccaugacua cuucauuggg      480

aggacagaua aucuuaccga cgcaaucaac aagcggcacg ccgagauuua uaagggacug      540

uuuaaagcug aauuguucaa uggcaaggua cucaaacaac uggguacugu gacaaccacg      600

gagcaugaaa acgcccugcu ccgcucauuu gauaaguuca cuaccuacuu uaguggguuc      660

uacgagaaua ggaaaaacgu cuuuagcgcg gaagacaucu ccacagcaau accccacaga      720

auugugcagg auaauuuccc uaaguuuaaa gagaacuguc acaucuucac ucgguuaauu      780

accgccguuc caucucugcg cgagcauuuc gaaaauguga agaaggcuau cggaaucuuu      840

gucucgacau caauugagga aguguucagc uuucccuucu auaaccagcu uuugacccag      900

acgcaaauag accuguacaa ucagcuacuc ggcggcauca gucgagaggc cgggacugag      960

aaaauuaagg gacugaacga aguacuuaac cuggcaaucc agaaaaauga ugagacagcu     1020

cacauaauug ccuccuugcc ucauagauuu aucccacucu ucaagcaaau ccugucugac     1080

aggaacaccc uuagcuuuau ucuggaagag uucaaauccg acgaagaggu uauccaguca     1140

uuuugcaagu auaagacucu ccuucggaau gaaaacgugc uggagacagc ggaggccuug     1200

uucaaugaac uaaacaguau ugaucugacc cacauauuua ucagccacaa aaagcucgag     1260

acgauuucuu ccgcacugug ugaccauugg gauacccuuc guaaugcucu guacgaacgc     1320

agaaucucag aguugacagg uaaaaucacu aagagcgcca aagagaaggu ccagaggagu     1380

cucaagcacg aagacauuaa ccugcaggag auaaucucug cugccggcaa agaauuaucc     1440

gaggcauuca agcagaaaac cagcgaaauu cugucgcaug cccacgcugc gcuagaucaa     1500

ccccucccua caacucugaa gaaacaggag gagaaggaaa uccuuaaguc ucaguuggac     1560

ucccugcucg ggcuguauca ucuucuggau ugguuugcag uggacgaguc aaaugaagug     1620

gauccagagu ucagugcccg guugaccgga auuaaacucg aaauggagcc gagccugucc     1680

uucuacaaca aggcucgaaa uuaugccacu aaaaagcccu acucugucga gaaauuuaag     1740

uuaaacuucc aaaugccuac ccuggcaagc ggcugggacg uuaauaaaga aaagaacaau     1800

ggugccaucc uauuugugaa gaacgggcuc uacuaucugg gaauaaugcc aaaacagaag     1860

ggccgcuaca aagcucuuuc auucgagccc acugaaaaga cgagugaggg cuuugauaaa     1920

auguauuacg acuauuuccc ugacgccgca aagaugaucc caaagugcuc cacacaguug     1980

aaagcgguaa ccgcucacuu ucagacucac accacaccca uucugcucag caacaauuuc     2040

aucgaaccgc uggagauuac caaggagauc uacgauuuaa acaauccuga aaaagagccc     2100

aagaaauuuc aaacugccua ugcaaagaag acaggggacc agaaaggaua cagagaagcc     2160

cuguguaagu ggauagauuu cacgagggac uuucuuucua aauacaccaa gacuacauca     2220

auugauuuga guagccuccg gccauccucu caguauaaag accuggguga guacuaugcu     2280

gagcuaaacc cucugcucua ccauaucucg uuccagcgca uugccgaaaa ggagaucaug     2340

gaugcagugg aaaccggcaa gcuguaucuu uuucaaauau acaauaaaga cuucgcuaag     2400

gggcaccaug gaaaacccaa cuugcacaca cuguauugga cuggcuuauu cagcccagag     2460

aaucucgcca agaccuccau caaacugaac ggucaggcgg aauuauuuua cagacccaag     2520

ucaaggauga aacguauggc ccaccggcug ggggagaaga uguugaauaa gaaacucaag     2580

gaucagaaaa cgccuauucc agacacccug uaccaagagc uauaugauua cgucaaccau     2640

cgacugaguc acgaccucuc ugaugaagcu agagcacugc uuccgaaugu gaucacaaag     2700

gagguuagcc augaaauuau caaagacagg cgcuucacuu ccgacaaguu uuucuuucac     2760

gugcccauaa ccuugaacua ucaggccgcu aauucaccua gcaaguucaa ccagcggguc     2820

aaugccuacc ugaaagagca uccagaaaca cccauuaucg gcaucgauag aggagagagg     2880

aacuuaauuu auaucacugu gauugacucu accggcaaga uacucgagca gcgcucccug     2940

aacacgaucc aacaguuuga uuaccagaaa aagcuggaca aucgagaaaa agagcgggua     3000

gcagcgcguc aggccuggag uguugugggg acaauuaagg aucuuaagca aggauaucua     3060

agccagguca uccacgaaau uguggaccuc augauccacu accaggcugu cgugguucug     3120

gagaacuuga auuucgguuu uaaaucuaag agaaccggca uagccgaaaa agcaguguac     3180

cagcaauucg agaagaugcu gaucgauaaa uuaaacugcc ucguacugaa ggacuauccu     3240

gcugagaagg ucgggggagu gcuuaaucca uaccagcuga cugaucaguu uacauccuuc     3300

gccaaaaugg gcacccaauc agguuucuug uuuuauguuc ccgcaccgua cacaaguaag     3360

auugacccuc ucaccggguu cguggaucca uuugucugga aaacaaucaa gaaccaugaa     3420

agcaggaaac acuuccugga gggcuuugac uuccuacauu augaugugaa gacgggagac     3480

uuuauucugc acuucaagau gaaucgcaac cuuucguuuc agcggggccu ccccggguuc     3540

augccugccu gggacaucgu auuugagaaa aaugaaaccc aguucgaugc uaagggaacu     3600

cccuuuauag cggguaaaag aauugugcca guuaucgaga accauagguu cacaggccga     3660

uaccgcgacc uguauccugc caaugaauug aucgcacugu uagaggaaaa ggggauuguc     3720

uuccgggaug gauccaacau acuccccaaa cugcuugaga augacgauuc ucacgcuauc     3780

gacaccaugg uggcccugau uagaagcgug uugcagauga ggaacucaaa ugccgcuaca     3840

ggcgaagauu acaucaacag uccaguccgu gaccucaacg guguuuguuu ugauucccgc     3900

uuccaaaauc cggaguggcc uauggacgca gaugccaacg gggcguacca cauugcucug     3960

aagggccagc uacugcuuaa ucaucucaaa gagucuaagg accugaaguu gcagaacgga     4020

aucagcaauc aggacugguu agccuauaua caagaacugc ggaacaaaag acccgcagcc     4080

acuaagaaag cuggccaggc aaagaaaaag aaguaaggac uagucccugu ucccagagcc     4140

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         4256


<210>  3553
<211>  4235
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3553
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaagc auuuaccagg aguucguuaa caaguauucc      180

cugucuaaaa cccucagguu ugaacuuaua ccacaaggaa agacacugga gaauaucaag      240

gcccgggguu ugauuuuaga cgaugaaaaa cgcgcgaagg acuacaaaaa ggcuaaacag      300

aucauugaua aguaucauca guucuuuauc gaggaaauac ugucaagugu gugcaucagc      360

gaggaccuac uccagaacua cuccgaugua uauuucaagc ugaaaaaguc ugacgaugac      420

aaucuucaaa aagauuuuaa gucggccaaa gacacuauua agaaacagau cucagaguac      480

auuaaggaua gcgaaaaguu caaaaaccug uuuaaucaga acuugaucga cgcaaagaaa      540

ggccaggaga gugaucucau acuguggcug aagcaaucca aagacaaugg gauugaacuc      600

uucaaggcca acucugacau cacggauauu gacgaggcuu uagagaucau caagagcuuu      660

aaaggaugga ccacauauuu caagggcuuu cacgaaaauc guaaaaacgu guacuccuca      720

aaugauauuc cgacaaguau aaucuaccga auugucgacg auaaccugcc caaguuccuu      780

gagaauaaag caaaguauga gagcuugaag gacaaagccc cugaggcuau caacuacgaa      840

cagauaaaga aagaucuggc cgaggagcua accuuugaca uugauuauaa gacaucugaa      900

gugaaucaga gaguuuucuc ccucgacgag guguucgaaa ucgcgaacuu uaacaauuac      960

cugaaccagu cagguaucac caaauucaau acuauuaucg ggggaaaguu ugucaacggc     1020

gagaauacaa agaggaaagg cauuaacgaa uauauaaauc uuuacagcca acagaucaac     1080

gauaagaccc ugaaaaagua uaaaaugagu guguuguuca agcagauucu cucugacacg     1140

gaguccaaga gcuuuguaau cgacaaacug gaggaugacu cggauguugu gacuaccaug     1200

caaucuuucu acgaacagau cgcagccuuu aagacagucg aggaaaaauc cauuaaggag     1260

acuuuaucac uccuguucga cgaucuuaaa gcucagaagc uggacuugag uaaaauauac     1320

uuuaagaaug auaagagccu aaccgaccug ucccagcaag uguucgauga cuauucugug     1380

aucgggacag ccguccucga auacauuacc cagcagaucg cacccaaaaa ccuggauaau     1440

ccaagcaaga aagagcagga gcuuauugcu aagaaaacgg aaaaggccaa guaucuguca     1500

uuggagacua ucaaacucgc gcuggaagag uuuaacaagc aucgggacau agauaaacaa     1560

ugucgcuucg aggaaaucuu agccaauuuu gcagcuauuc cuaugaucuu cgacgagauu     1620

gcccagaaca aggacaaucu ggcucagauc aguauaaaau accagaacca aggaaagaag     1680

gaucuacucc aggccuccgc agaagacgau guuaaagcca uuaaggaccu gcuugaucag     1740

acaaacaauu ugcugcacaa acucaagauc uuccauauua gccagucuga ggacaaagcu     1800

aacauccugg auaaggacga acacuuuuau cuuguguucg aggagugcua cuuugaacug     1860

gcgaauauag uaccauugua uaacaaaauc agaaauuaca uuacccaaaa gcccuacuca     1920

gaugagaagu ucaaacucaa cuuugaaaau aguacucugg caaacgguug ggacaagaau     1980

aaagagccug auaacacagc caucuuauuc auuaaggacg auaaauauua ccugggcgug     2040

augaauaaga agaacaauaa aaucuuugac gacaaggcua uaaaagaaaa caagggggag     2100

ggauauaaaa agauugucua caagcuacuc ccaggcgcca auaaaaugcu gccgaaggug     2160

uucuuuagcg caaaauccau caaguucuau aaccccucug aggauauccu uaggauucgg     2220

aaccacucga cccauacgaa aaaugguagc ccucagaagg gguacgaaaa guuugaguuc     2280

aacaucgaag acugucgcaa auuuauugau uucuauaagc aguccauauc aaaacaccca     2340

gaguggaagg acuucggauu ucgauucagu gauacccaaa gauacaauuc uaucgacgaa     2400

uuuuacaggg agguugagaa ccagggcuau aaauugacau ucgaaaauau uagcgagucc     2460

uacaucgauu caguggucaa ccagggcaag cuguaucucu uucagauuua caauaaggac     2520

uucagcgccu auucuaaagg gcggcccaac cugcauacuu uauacuggaa ggcucuguuu     2580

gaugaacgua aucuucaaga cgugguauau aaauugaacg gagaggccga acucuucuac     2640

cgcaagcagu ccaucccuaa aaagauaacc cacccagcaa aagaagcgau cgcuaauaag     2700

aacaaggaua aucccaaaaa ggagaguguu uuugaauacg accugauuaa agacaagaga     2760

uucacagagg auaaauuuuu cuuccacugc ccgaucacua uuaacuuuaa gagcucuggu     2820

gccaauaagu ucaacgacga aaucaaccua cugcucaaag agaaggcaaa ugaugugcau     2880

auacugucca uugacagggg cgagcggcac cuugccuauu acaccuuggu cgaugggaaa     2940

ggaaacauca ucaagcagga cacauuuaau auuauaggca acgaucgaau gaaaaccaau     3000

uaucaugaca agcuggcugc caucgaaaag gaucgcgacu cagcaagaaa agauuggaag     3060

aaaauuaaca auaucaagga gaugaaagaa gguuacuuaa gucagguggu ccacgagauu     3120

gcuaagcucg ugaucgaaua uaacgccaua guuguguucg aggaccugaa uuuuggguuc     3180

aagaggggcc gguuuaaagu agagaagcaa gucuaccaga aacuugaaaa gaugcugauc     3240

gagaaauuga acuaucucgu guucaaggau aaugaauuug acaaaacugg aggcguucug     3300

cgcgcguacc agcuaacggc cccuuucgag acauuuaaga agauggggaa acagaccgga     3360

auuaucuacu augugcccgc ugguuucacu agcaagauuu guccagucac cggcuuugug     3420

aaccaacugu acccuaaaua ugaaucggua uccaagucuc aggaguucuu cagcaaauuu     3480

gacaagaucu gcuacaaucu cgauaagggg uauuucgagu uuucauucga cuacaaaaac     3540

uuuggagaua aggcagccaa aggcaagugg acaauagcua guuucgguuc cagacugauu     3600

aauuuuagga acucugacaa aaaucauaac ugggauaccc gugaagugua ucccacuaag     3660

gagcuugaaa aguugcugaa agacuacagc aucgaguacg ggcacggcga guguaucaag     3720

gccgcaauuu gcggagaauc cgauaaaaag uucuuugcga aauuaacauc aguucucaac     3780

acgauccugc agaugcggaa uagcaagacc ggcacugagc uugacuaucu gauaagucca     3840

gucgccgaug ugaacgggaa uuucuuugac ucucgacaag cucccaagaa caugccucag     3900

gaugccgacg caaauggagc uuaccacauu ggucuaaaag gccucaugcu guuggggaga     3960

aucaagaaca aucaggaagg caaaaagcug aacuuaguga uuaaaaauga ggaauauuuc     4020

gaguuugucc agaacaggaa uaacaagcgc ccagccgcaa cuaaaaaggc cggacaagcu     4080

aagaaaaaga aauaaggacu agucccuguu cccagagccc acuuuuuuuu cuuuuuuuga     4140

aauaaaauag ccugucuuuc agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                4235


<210>  3554
<211>  4420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3554
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaga caagaaauac agcauuggac uggauauagg uacaaacucc guuggcuggg      180

ccgugaucac agacgaguau aagguaccau cuaagaaauu caaggugcuc gggaauacug      240

auaggcauuc aauuaaaaag aaccuuaucg gagcgcuguu guuugacagu ggcgaaacgg      300

cugaggccac ccgguuaaaa cgcacagcac gacguagaua cacuaggcgg aagaaucgca      360

uuugcuaucu gcaggaaauc uucagcaacg agauggccaa ggucgaugac uccuuuuucc      420

acagacuaga agagucuuuu cucguggagg aagauaaaaa gcaugagagg cacccgauau      480

ucgguaauau cguugacgaa guggcuuacc augagaaaua ucccaccauu uaccaucugc      540

ggaagaaacu ugucgauucg acagacaagg cagaucugcg cuugaucuau cucgcccugg      600

cucacaugau uaaauuucga gggcacuucc ugaucgaggg agaccucaac ccugauaauu      660

cagacgugga caaguuauuu auacaacugg uacagaccua caaccagcuu uucgaagaga      720

auccaauuaa cgccagcggc guugaugcga aggcaaucuu gagugccaga cuguccaaau      780

cuaggcggcu agaaaaucuc auugcucagc ugcccggcga gaagaaaaac gggcuuuuug      840

gaaaucugau cgccuugagc cucggucuga cuccuaacuu caaguccaau uuugacuuag      900

cagaagaugc uaaacugcaa cucucaaagg acacauacga ugacgaucuu gacaaccugu      960

uggcccagau cggcgaucag uaugcggacc uauuccuggc cgcaaagaac cucagugaug     1020

cuauucugcu uagcgacaua cugcguguga auaccgagau cacgaaagcc ccauugucug     1080

cuuccaugau uaagcgcuac gacgagcacc aucaggaucu cacucuguua aaagcccugg     1140

ucagacaaca gcuacccgaa aaguauaaag agaucuucuu ugaccaguca aagaacgggu     1200

acgcaggaua uauagauggc ggugccagcc aggaagaguu cuacaaguuu auuaaaccua     1260

uccucgaaaa gauggacggg accgaggagc ugcuugugaa auugaauagg gaagaucugc     1320

uccggaagca acgaacauuc gacaacggaa guaucccaca ccagauucac cugggcgagc     1380

uucaugcuau ccugcgcaga caggaagauu uuuauccguu cuugaaagac aauagggaga     1440

agauugaaaa gauacucacu uuucggaucc ccuacuacgu gggcccucug gcgcgcggga     1500

acucuagauu cgcauggaug accaggaaau ccgaggagac aauuacccca uggaauuuug     1560

aagaggucgu ugauaaggga gccagcgcuc aaucguucau cgaacguaug acgaacuuug     1620

acaaaaauuu acccaacgag aaggugcugc cuaaacacuc ucuacucuau gaguacuuca     1680

cuguauauaa ugaacugaca aaggugaaau acgucaccga ggguaugcgg aagccagccu     1740

uucuuuccgg cgaacagaag aaagcaaucg uggauuugcu guucaagacu aaccgaaaag     1800

uuacagugaa gcagcucaaa gaggacuauu ucaagaagau ugaauguuuu gauucagucg     1860

agauaagugg gguggaggac agauucaaug ccagccuggg aaccuaccau gacuuacuga     1920

aaaucauuaa ggauaaagac uuucuugaua acgaagagaa ugaagacauc uuggaggaua     1980

uuguacucac gcugacccua uucgaagaca gggagaugau cgaggaacgc cugaagacau     2040

augcucaccu cuuugaugac aaaguuauga agcagcugaa gcggagaagg uacacuggcu     2100

ggggucgccu uucccgaaaa uugauaaacg ggauccggga uaagcaaucu ggcaaaacca     2160

uucuggacuu cuuaaagagc gauggauuug ccaaccguaa uuucaugcag cucauccaug     2220

acgauucacu gacauuuaaa gaggacauuc agaaggcaca ggugaguggc caaggggacu     2280

cccuucacga acacaucgcg aaccuggcug gaagccccgc cauaaagaaa gguauuuugc     2340

agacugucaa gguggucgau gagcucguga aaguuauggg cagacauaag ccggaaaaua     2400

ucgugauuga gauggcaagg gagaaccaga ccacacagaa agggcaaaag aacucucgcg     2460

aacggaugaa aagaaucgag gaaggaauaa aggagcuggg cucacagauc cuaaaggagc     2520

acccuguaga aaauacccag cugcagaaug agaaacucua ccuguauuac cuucaaaacg     2580

guagggacau guaugucgau caggaauugg acauuaaucg acugagugau uacgacgugg     2640

aucauaucgu uccccagagc uucuuaaagg acgauuccau ugacaacaaa gugcucacuc     2700

gcucugauaa gaaucggggg aaaucggaca acgucccaag cgaggaagug guaaagaaga     2760

ugaaaaauua uuggagacaa cugcuuaacg ccaagcugau cacgcagagg aaauuugaca     2820

aucuaacaaa ggcugagcgu ggcggacucu ccgagcugga uaaagccggc uucauaaagc     2880

gccaguuggu ggaaacccgg cagauuacua agcacguugc acaaauccug gacucaagaa     2940

ugaacaccaa auacgaugag aacgacaagu uaaucaggga agucaaagug auuacacuca     3000

agaguaaacu ggugucugau uuucgaaagg acuuccaguu cuauaagguc cgggagauca     3060

auaacuacca ccaugcucac gaugccuacc uuaaugcggu uguggggacc gcccugauua     3120

aaaaguaucc uaaauuggaa agcgaguuug uguacggaga cuauaaggua uacgaugucc     3180

gcaaaaugau agcuaagucc gagcaggaaa ucgguaaggc aacugccaaa uauuucuuuu     3240

acucaaacau uaugaauuuc uuuaagacag agaucacgcu cgcuaacggc gaaauuagaa     3300

aaaggccccu gaucgagacc aauggggaaa cuggagagau agugugggac aagggccggg     3360

auuucgccac aguucgaaaa gugcuaagca ugccacaggu caacaucgug aagaaaaccg     3420

agguucaaac agguggguuu ucuaaggaau ccauucugcc caagagaaau agugacaaac     3480

uuaucgcacg caagaaagau ugggacccua agaaauaugg cggauucgac agcccaacug     3540

uggcguacuc uguacucguc guggccaagg uggagaaggg caaauccaag aaacugaagu     3600

cagucaaaga auugcugggg auuaccauca uggagaggag uagcuuugag aagaacccga     3660

uagauuucuu agaagcuaag ggauacaaag agguuaagaa agaccucauu aucaagcugc     3720

ccaaauauuc gcuuuuugaa cuggagaaug gccguaagcg gauguuggcc uccgcuggug     3780

aacuccagaa ggggaacgag cuggcacuac cuucuaaaua cgugaauuuc cuguaucuug     3840

ccagccauua cgagaagcuc aaaggaucac cagaagauaa cgagcagaag cagcuguucg     3900

uagaacaaca caaacauuau uuggacgaga ucauugaaca gauaagugag uuuuccaaga     3960

gagugaucuu agcagaugcc aaucuggaca aaguccucuc ugcuuacaac aagcacaggg     4020

auaagcccau ucgcgagcag gcggaaaaca ucauucaccu guucacgcuu accaaucugg     4080

gcgccccugc agcuuuuaaa uauuucgaca caacuaucga ucgaaagaga uacaccagca     4140

caaaagaggu guuggacgcc acucuaauac aucaauccau caccggucug uacgaaacgc     4200

ggauugaucu cucacagcug ggcggggaca agaggccagc cgcuaccaaa aaggcaggac     4260

aggccaagaa aaagaaauaa ggacuagucc cuguucccag agcccacuuu uuuuucuuuu     4320

uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                           4420


<210>  3555
<211>  5203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3555
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa cuucaagauu cugccaauag ccaucgaccu cggaguuaaa aauaccggug      180

uguuuagcgc guucuaccag aagggcacau cccuugagag gcuggauaac aagaauggga      240

aaguauauga auugucuaag gacucauaca cuuuacugau gaacaaucgg acggcucgcc      300

gacaucaacg uagaggaauu gauaggaaac agcuagugaa gcggcucuuu aaacugaucu      360

ggaccgagca gcuuaaccug gaaugggaca aggauacaca gcaagccauu aguuucuugu      420

uuaaucgcag aggcuucagc uuuaucacug acgguuauuc cccggaguac cucaacauag      480

uccccgaaca ggugaaggca auccugaugg auauuuucga cgauuauaau ggggaggacg      540

aucuggacuc uuaccucaaa uuagccaccg agcaggaauc gaagaucuca gagauuuaua      600

acaaacugau gcagaagauc cuugaauuua aauugaugaa gcugugcaca gauauaaaag      660

acgacaaggu uagcaccaag acucuaaaag agauuacaag uuacgaguuc gaacuccugg      720

cugauuaccu ugcaaauuau uccgagucuc ugaagaccca aaaauuuagc uacacggaca      780

agcagggaaa cuugaaagaa cucuccuauu accaccauga uaaguauaau auccaggagu      840

uccugaagag gcacgccacu auuaacgacc ggaucuuaga uacccugcuc acagacgauc      900

uugacaucug gaauuuuaac uucgaaaaau ucgauuuuga caagaacgag gagaaacugc      960

agaaucaaga agauaaggac cauauucagg cucacuugca ccauuucgug uuugccguca     1020

acaaaauaaa gucagagaug gcgaguggcg gccgccaccg aagccaguac uuccaagaaa     1080

ucacuaaugu gcuagacgag aacaaucauc aggaagggua ucugaagaac uuuugugaga     1140

aucuccacaa caaaaaguac ucuaaucugu ccguuaaaaa ccuuguaaau cugauuggaa     1200

acuugucaaa ucucgagcug aagccuuuaa gaaaauacuu caacgauaag auccacgcaa     1260

aggccgacca uugggaugaa cagaaauuua ccgagacaua uugccacugg auacugggug     1320

aauggagggu gggcgucaag gaccaggaua aaaaggacgg ggcuaaauac agcuauaagg     1380

aucuauguaa ugagcucaaa caaaagguga ccaaggccgg acugguggac uuccuuuugg     1440

aacuggaucc augccggacg auucccccuu accucgacaa caacaaucgu aaaccaccca     1500

agugucagag ucugauccuu aacccuaaau uucuggauaa ucaguaucca aacuggcagc     1560

aauacuugca ggagcucaag aaacugcagu cuauccagaa uuauuuagac uccuucgaga     1620

cugaucugaa gguccuaaag agcucgaaag accaaccgua cuuuguugaa uacaagucuu     1680

ccaaccagca gauugcauca ggccagcgcg acuauaaaga ucucgacgcu agaauccugc     1740

aauucauuuu ugauagggug aaggccagug acgagcuuuu gcugaaugaa auauacuucc     1800

aggcgaaaaa gcucaagcag aaagccagcu ccgagcugga gaaguuagaa ucuagcaaaa     1860

agcuggauga gguaaucgca aacucacaac uuagucagau uuugaaaucc cagcauacaa     1920

augguaucuu cgaacagggg accuuucucc accuggugug caaguauuac aagcaacggc     1980

agcgagcucg cgacagcaga cuauauauca ugcccgagua cagguaugau aaaaagcugc     2040

auaaauacaa caauacugga cgguucgacg augacaacca gcuccugaca uacuguaauc     2100

acaagccucg ccagaaaaga uaucaacuuu ugaacgaucu ggccggcguc uuacaggugu     2160

cuccaaauuu ucucaaggac aaaauuggcu cagaugacga ccuguucaua aguaaguggc     2220

uuguugaaca caucaggggg uuuaagaaag caugcgagga uagccugaag auucagaaag     2280

acaaccgugg auugcucaac cauaagauca auauugcccg gaacaccaaa gguaagugug     2340

agaaggaaau cuucaaucug auaugcaaaa ucgagggcuc cgaagauaag aaagggaacu     2400

acaagcacgg acuagcauau gagcugggcg ugcuccuguu uggugaaccc aaugaggccu     2460

cuaaaccuga guucgaccga aagauuaaga aauuuaacuc gaucuacagc uucgcucaga     2520

uucaacagau cgcguuugca gaaagaaagg ggaaugccaa cacgugugcu gucugcuccg     2580

ccgauaaugc acauaggaug cagcaaauaa aaauuaccga gccaguggaa gacaacaagg     2640

auaaaaucau ucuuucagcc aaggcucagc gcuugcccgc caucccgaca cggauaguag     2700

acggcgcagu uaagaaaaug gcgacuaucc uggcuaagaa uauuguggau gacaacuggc     2760

agaauaucaa acaggucuua agugccaagc accaacucca cauuccuauc auaaccgagu     2820

cuaacgcauu cgaauuugag cccgcccugg cugaugugaa aggaaagagc cuuaaggaca     2880

gaaggaaaaa ggcccuggag cgcauuuccc cagaaaauau cuucaaagac aagaacaacc     2940

gaaucaaaga guucgcaaag ggcauuucag cuuauagcgg ggccaaucua acagauggag     3000

acuuugaugg ugcgaaagaa gaacucgacc auaucauucc ucggucucac aagaaguacg     3060

gcacucugaa cgaugaggcc aauuugauau gcgucacccg uggggacaac aaaaauaagg     3120

gaaacagaau cuucugucug agggauuuag cugacaauua uaaacucaag caguuugaaa     3180

caaccgauga ccuggagauu gagaaaaaga ucgcagauac uauuugggac gccaacaaga     3240

aagauuucaa guuuggcaau uaccgcuccu ucaucaaccu uacgccccag gagcagaagg     3300

cuuuucggca ugcccuguuc uuggcagacg aaaauccaau aaaacaagcg gugaucagag     3360

ccauuaacaa uaggaaccga acauuuguua augguaccca gcgcuacuuc gcugaggugc     3420

ucgccaacaa uaucuaucug cgggcuaaaa aggagaaccu aaacacugac aagauuaguu     3480

uugauuacuu cgggaucccc accauaggca auggaagagg cauugcugaa aucaggcagc     3540

uguaugagaa aguagacagc gauauccagg ccuacgcaaa gggggacaaa ccucaagccu     3600

cuuauuccca ccuuauugau gcuaugcucg cguucugcau agccgcagac gaacaucgua     3660

acgauggauc aaucggucug gagauugaca agaauuacag uuuguaucca cuggauaaaa     3720

acacaggcga agucuuuacc aaggacaucu ucagccagau uaagaucacu gauaaugagu     3780

uuucggacaa aaaguuagug cgcaaaaagg cuauagaggg guucaacaca caccggcaga     3840

ugacgagaga uggaaucuac gccgaaaauu accucccgau ucugauccac aaagagcuua     3900

acgaaguuag gaagggcuau accuggaaaa auuccgagga aauuaagauc uuuaagggua     3960

aaaaguacga cauacagcaa cugaacaauu ugguguauug ucucaaauuc gucgacaagc     4020

ccauuucuau cgauauccag auuagcacuc uggaggagcu acgaaacauc cugacaacca     4080

auaacauagc cgcuacagca gaauacuauu acauuaaucu uaaaacucag aagcuccaug     4140

aguauuacau cgaaaacuac aacaccgccc ugggguauaa gaaauacuca aaggagaugg     4200

aguuuuugcg gaguuuagcg uaucgcuccg aaagagugaa aauuaagucu aucgacgaug     4260

uaaaacaagu gcuggacaag gauagcaauu ucauaauugg caagaucacg cucccuuuua     4320

aaaaggagug gcagaggcug uaccgggaau ggcagaacac cacaaucaaa gacgauuaug     4380

aguuccuuaa guccuuuuuc aauguuaaau caauuacuaa gcugcacaag aaaguccgaa     4440

aggacuuuag cuugccaauc aguaccaacg aaggaaaauu ccuagugaag agaaaaacau     4500

gggauaauaa cuucauuuac cagauacuga augacucuga uucccgcgcu gacggcacua     4560

agcccuuuau cccugccuuc gacauuagca aaaacgagau cguggaggca aucauugauu     4620

cguuuaccuc aaagaauaua uucuggcucc caaagaacau cgaacugcaa aaagucgaca     4680

auaagaacau uuuugccauc gauacgucua aaugguucga gguugaaaca cccagugacc     4740

uuagggauau ugggaucgcu accauacagu auaagaucga caauaacucc cguccgaaag     4800

ugcgggugaa guuagauuac guaauugacg augacagcaa gaucaauuac uuuaugaacc     4860

auucucugcu caaaagcaga uauccugaua aggucuugga gauucugaaa caguccacua     4920

ucauagaguu cgaaucaagu ggauuuaaca agacaauuaa agagaugcuc ggcaugaagc     4980

uggcagguau cuacaaugaa accagcaaca auaagaggcc agccgcgacu aaaaaggcug     5040

ggcaggccaa aaagaaaaag uaaggacuag ucccuguucc cagagcccac uuuuuuuucu     5100

uuuuuugaaa uaaaauagcc ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     5160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                       5203


<210>  3556
<211>  3475
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3556
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaggaacuac auucugggac ucgacauagg uaucacauca guuggcuaug      180

ggauuaucga uuacgagaca cgggacguga uugaugccgg aguacgccuu uucaaagaag      240

cgaaugugga gaacaaugaa ggccgacguu ccaagagagg ugcuaggcgg cugaagcgca      300

gaaggcggca ucgcauccag cgagucaaaa aguuguuauu ugacuauaac cugcuaacug      360

aucacucuga gcucucaggg auaaauccau acgaagccag agugaaagga cugagucaaa      420

aacuuagcga ggaggaguuc uccgcagccc uguugcaucu cgcuaaaagg cggggcguuc      480

acaacgugaa ugaagucgaa gaggacacgg gcaacgagcu gucuaccaag gaacagaucu      540

cgcguaauuc aaaggcacug gaggaaaaau auguggccga gcuccaguua gaacgccuga      600

agaaagaugg ggagguaaga ggaagcauua acagguuuaa gacaagugac uacguuaaag      660

aggcuaagca gcuuuugaaa gugcaaaagg ccuaucauca gcuggaucag uccuucaucg      720

acacuuacau ugaucuacuc gaaacccggc gaacauacua ugaggguccg ggcgaagggu      780

cucccuuugg auggaaggac aucaaagagu gguacgaaau gcugaugggc cacugcaccu      840

auuucccuga ggagcuucgc agcgucaagu acgcguauaa ugcagaucug uacaacgccu      900

ugaaugaccu caacaaucug gugauaacua gagacgaaaa cgagaaauua gaauauuacg      960

agaaguuuca gauuaucgaa aacguguuca aacaaaagaa gaaaccaaca cugaagcaga     1020

uugcuaaaga gauccucguc aaugaggaag auaucaaggg uuacaggguu accuccacgg     1080

ggaaacccga guuuacuaac cuuaaggugu aucacgacau uaaggauaua accgcccgga     1140

aagaaaucau ugagaaugca gagcuguugg accagaucgc uaagauacua acaauuuacc     1200

agucaaguga agauauccaa gaggaacuga cuaaccucaa uagcgagcug acccaggaag     1260

agaucgagca gauuucuaac cuuaaaggau auacaggcac ccauaaucug uccuugaagg     1320

ccaucaaccu cauucuggac gaauuauggc acacgaauga uaaccaaaua gcgaucuuca     1380

aucgccugaa acuuguaccu aagaaggugg accucucaca gcagaaagag auuccaacua     1440

cacuggucga ugacuuuauc cuuagccccg ugguuaagag aaguuucauc cagucuauua     1500

aagugauaaa cgccaucauu aagaaauacg gcuugccuaa ugauaucauu aucgaacugg     1560

caagggagaa gaacuccaaa gacgcucaaa agaugauaaa ugaaaugcag aagcguaacc     1620

ggcagaccaa cgagcgaauc gaggaaauua ucagaacuac agggaaagag aaugccaagu     1680

aucucauuga aaagaucaag cugcaugaca ugcaggaggg aaaaugucuu uacagccugg     1740

aagcuauacc auuggaggau cuccugaaca auccguuuaa cuaugagguc gaccacauua     1800

uccccagguc ggugucuuuc gauaauuccu ucaacaauaa gguauuaguu aagcaagaag     1860

agaacucaaa gaaggguaau cgcaccccuu uucaguaccu gaguagcucc gacucuaaaa     1920

uuagcuacga aacguucaag aaacacaucc uaaaccucgc caagggcaag gggcggauau     1980

caaaaaccaa gaaagaguau cugcuugagg aaagagauau caauagguuu agugugcaga     2040

aggacuucau uaaccgcaau uuggucgaua cacgauacgc aacucgggga cugaugaacc     2100

uccugcguuc cuauuuuaga gugaauaacu uagacgucaa agugaaaagc aucaacggcg     2160

guuucaccuc uuuucugagg cgcaagugga aauucaagaa agagcggaau aagggguaca     2220

aacaucacgc cgaagaugcu cuuauuaucg cgaacgcaga cuuuauauuc aaggagugga     2280

aaaaguugga uaaggccaaa aagguuaugg aaaaucagau guuugaggag aaacaagcug     2340

aaucaaugcc agagauugaa acagagcagg aauauaagga gaucuucauc acuccccauc     2400

agauuaaaca caucaaggac uuuaaggauu acaaauauag ucauagagug gacaagaaac     2460

cuaacaggga gcucauuaau gacacccugu acagcacacg aaaggaugac aaaggcaaca     2520

cccuaauagu aaauaaccug aauggacucu acgauaagga caacgauaag cugaaaaagc     2580

uaaucaauaa auccccagaa aaguugcuga uguaucacca cgacccccag acuuaccaaa     2640

aauuaaagcu cauuauggag caguauggcg augaaaagaa cccgcuguac aaauauuacg     2700

aggaaacggg gaauuaucuu acaaaguacu cuaaaaagga caacggaccu gucaucaaaa     2760

agauuaagua cuaugguaau aaacugaacg cccauuugga uaucaccgac gauuacccca     2820

acucgcgcaa uaaggugguu aaacucagcc ugaagccaua ucgguucgac guguaccuag     2880

auaacggcgu cuauaaauuc gugacuguaa agaaucugga cgugaucaaa aaggagaacu     2940

acuaugaggu uaauuccaag ugcuacgaag aggccaaaaa gcucaaaaag auaucaaacc     3000

aggcagaauu uauugcuagu uucuacaaua acgaccugau caaaauuaau ggggagcuuu     3060

auagagucau cggagugaac aaugauuugc ugaacaggau agaggugaau augauugaca     3120

ucaccuaccg ugaauauuua gagaacauga augauaagcg cccuccccgg aucauuaaga     3180

caauagccuc uaaaacccaa agcaucaaga aauacuccac ugacauucuc ggcaaccugu     3240

augaagucaa gagcaaaaag cacccacaga ucauuaagaa agguaagaga cccgcagcga     3300

ccaaaaaggc ugggcaggcc aaaaagaaga aauaaggacu agucccuguu cccagagccc     3360

acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa aaaaaaaaaa     3420

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu          3475


<210>  3557
<211>  4543
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3557
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcacu guucaacaag ugcauuauaa ucagcauuaa ucucgacuuu uccaacaaag      180

agaaguguau gaccaagcca uacucuaucg gacuugauau ugguacaaau ucaguuggcu      240

gggccgugau cacugacaac uauaaaguac cgaguaagaa aaugaaggug cuggggaaua      300

cgagcaaaaa guacauaaag aaaaacuugu uaggaguccu gcuauucgau uccggcauca      360

ccgcggaagg uaggcggcuc aagcgcacag cucgacguag auauacuagg cggcgcaaua      420

ggauucugua ccuucaggag aucuuuucua ccgaaauggc cacacuggac gaugcauucu      480

uucaaagauu ggacgauucg uuccucgugc ccgacgauaa acgggacuca aaguauccua      540

uuuuugggaa ccugguugag gaaaaagugu accaugauga guucccaacc aucuaucauc      600

ugcgcaagua ccucgccgac agcacuaaaa aggcugauuu acgacugguc uaccuugcau      660

uggcccacau gauaaaguau agaggacacu uucugauuga gggcgaguuc aauaguaaaa      720

acaaugacau ccagaagaac uuucaggacu uccuagauac auacaaugcu auuuuugagu      780

ccgaccucuc ucuggaaaac agcaaacagc uugaggagau cgugaaggau aaaaucucca      840

agcuggaaaa gaaagacagg auuuugaaac ucuuccccgg cgagaaaaau ucagggauau      900

ucagugaauu ucugaaguua aucguaggaa accaagccga uuuccggaaa ugcuuuaacc      960

uggacgagaa ggcgagccuc cauuucucua aggaauccua ugaugaggac cuugagaccc     1020

uguuggguua cauuggcgau gacuauucag auguuuuucu aaaagcaaag aaacuguacg     1080

acgccauccu ccugagcggg uuccuuacgg ugacugacaa ugaaaccgag gcuccucuga     1140

guucugccau gauaaagcgu uauaacgaac acaaagagga uuuggcacuc cugaaggaau     1200

acauucgcaa uaucuccuua aagacauaca acgaggucuu uaaagacgau acuaagaaug     1260

gauaugcugg cuacaucgac gguaaaacca accaggagga uuucuaugug uaccugaaga     1320

aucuacucgc cgaauuugag ggggcggacu auuuccugga aaagauugau agagaggacu     1380

uucuuaggaa gcagcggaca uucgauaacg gaagcauccc auaccagauu cacuugcaag     1440

aaaugcgagc cauacuggac aaacaggcaa aguuuuaucc cuuccucgcu aagaauaaag     1500

agcgcaucga gaagauucug accuucagaa ucccuuacua cgugggccca cuugccaggg     1560

gcaacucgga uuuugcuugg ucuauccgga aacgcaauga aaagauuacg ccguggaacu     1620

ucgaggacgu cauagauaaa gaauccucag ccgaggcauu uaucaauaga augacaaguu     1680

ucgaccugua uuugcccgag gaaaagguuc ucccuaagca uagccuguua uacgagacuu     1740

uuaacgugua uaaugaacug accaaaguaa gguucauugc cgaguccaug cgugacuacc     1800

aguuucuaga uucuaagcag aaaaaggaca ucgugcggcu cuauuucaaa gauaagcgaa     1860

aggucacuga caaagauauu aucgaauacc ugcacgcuau auauggguac gacggaaucg     1920

agcuuaaggg uauugagaaa caauuuaaca gcucauugag uacauaccau gaucugcuca     1980

acaucauuaa ugacaaggag uuccuggaug acuccagcaa cgaagcgauc auagaagaga     2040

uuauccacac cuuaacgauu uuugaagaua gagagaugau caaacagagg cugucuaagu     2100

ucgagaauau auuugacaag ucagugcuua aaaaguugag ucgccggcac uauaccggcu     2160

gggggaaacu cagcgcaaag cugaucaacg gaauuagaga ugaaaaaucc ggcaauacaa     2220

uccuagacua ccugauugac gaugguaucu cuaacaggaa uuucaugcag cucauacaug     2280

acgaugcccu gucguucaag aaaaagauuc aaaaggcuca gaucaucggg gacgaggaua     2340

aaggcaacau uaaggaaguu gugaaaagcc uuccaggauc ccccgccauc aagaaaggca     2400

uuuugcaguc aauaaagauc gucgacgagc uggugaaggu aaugggaggg cgcaaaccug     2460

aaaguauugu uguggagaug gcacgagaga aucaguauac uaaccaaggu aagucuaaua     2520

gccagcagcg guuaaaacgu cucgaaaagu cccugaaaga gcuuggcuca aagauccuga     2580

aggaaaacau uccagccaaa uugagcaaga ucgauaauaa cgcucuccag aaugacagac     2640

uguaccuaua uuaccugcaa aacgggaaag auauguauac cggagacgau cucgacauag     2700

acaggcuguc uaacuacgau aucgaccaca uuauccccca ggccuuucuu aaggauaauu     2760

ccauugacaa caaagucuug gugaguagcg caucuaaucg cggcaagucc gaugacuucc     2820

cgucacugga ggucgugaag aaacggaaga cauuuuggua ccaguuacuc aaaaguaagc     2880

ugaucagcca gagaaaauuc gauaaccuua cuaaggcgga gagggguggg cugcuaccug     2940

aagacaaggc uggcuuuaua caacgacagc ucguugagac ccgccagauu acaaaacaug     3000

uggcccggcu guuggaugaa aaguucaaua acaaaaagga cgagaauaac agagcaguaa     3060

ggaccgucaa aaucaucacu cugaagucga cguuaguguc ccaguuucgu aaagauuucg     3120

aacucuauaa gguucgcgag auuaaugacu uucaccaugc ccacgaugcu uaccugaacg     3180

ccgugauagc aucugcccuu cugaagaaau aucccaaguu ggagccagag uucgucuacg     3240

gagacuaucc uaaauacaau agcuuucggg aaagaaaguc agcuacagaa aaaguguauu     3300

ucuacaguaa caucaugaau auuuucaaga aguccaucuc ucucgcggac ggcaggguaa     3360

uugagcgacc ccugaucgaa gugaacgagg agaccgggga aagcguuugg aauaaagagu     3420

ccgaucuagc cacuguccgg cgcgugcugu cauacccaca agugaacguc guuaagaaag     3480

uggaagaaca gaaccacgga cuugacagag guaagcccaa aggccucuuu aaugcuaacc     3540

ugagcaguaa gccuaagcca aauucuaacg aaaauuuggu gggggcaaaa gaguaucugg     3600

auccgaagaa auacggaggc uaugccggua uauccaacag cuucgcugua uuagucaagg     3660

ggaccaucga gaaaggcgcc aagaagaaaa uuacaaaugu gcucgaauuu cagggaaucu     3720

cgauucugga caggaucaac uaccggaagg auaaacuuaa uuuccuguug gagaagggcu     3780

auaaagacau agaacucauu aucgagcugc ccaaguacuc acuauuugag cugucugaug     3840

ggagucgaag aaugcuugca uccauccuca gcaccaacaa uaaacgcgga gaaauucaua     3900

agggcaacca aaucuuccug ucucagaagu uuguuaaauu guuauaucac gcgaagagga     3960

uaagcaauac uauuaacgag aaucaucgua aauacgugga aaaccacaag aaagaguucg     4020

aggaacuguu uuacuauauc cucgaguuca augaaaacua cgucggugcc aagaagaacg     4080

ggaaacugcu uaauuccgcu uuucagucau ggcagaacca caguauugac gagcugugua     4140

gcucuuucau cggaccuaca ggcuccgaac ggaaggguuu guuugagcua acgucaagag     4200

gcagugccgc agauuucgag uuccuggggg ugaaaauacc aagguaucgc gacuacaccc     4260

ccagcucccu ccugaaggau gcuacucuua uucaucaauc uguuacagga uuauaugaaa     4320

cccgaaucga ccuggccaaa cucggcgagg ggaagagacc ugcagccaca aagaaagcug     4380

gacaggcgaa gaaaaagaaa uaaggacuag ucccuguucc cagagcccac uuuuuuuucu     4440

uuuuuugaaa uaaaauagcc ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                       4543


<210>  3558
<211>  3619
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3558
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaug guacgccagc cugauguccg cgcaucaccu caggguugga auugacgugg      180

guacccauuc uguaggccuu gcuacacugc ggguggauga ccacgggacu ccaauagagu      240

uguuaucagc ccugagucau auccacgaua gcggagucgg caaggaaggu aaaaaggacc      300

acgauacgcg caagaaacua uccgggauug cacgacgugc cagaaggcuc cugcaucacc      360

ggcgcaccca gcuucaacag cuggacgagg uguugagaga ucucggauuc ccgaucccca      420

caccuggcga auuucuggac cugaacgagc agacugaucc auauaggguu uggcgggugc      480

gcgcucgacu cgucgaagag aaguuacccg aggaacugag aggcccugca auuucuaugg      540

ccgugaggca uaucgcucgg caccgugggu ggcgcaaucc auacucgaaa guagagucac      600

uuuugagccc cgccgaagag aguccuuuca ugaaggcgcu gagagagagg auacuagcaa      660

ccacaggaga aguucucgac gaugguauca ccccaggcca ggccauggcu caaguggccc      720

ugacucacaa cauuuccaug cgggggccgg agggaauccu uggcaaacug caucagucug      780

acaaugcaaa cgaaauucga aagaucugcg cucgccaggg ugucagcccc gaugugugua      840

agcaguugcu cagagccgug uuuaaagcgg acuccccuag ggggucagcc gucagucggg      900

uugcaccaga cccccugccu ggacaaggca gcuuccgcag agcuccaaag ugcgaucccg      960

aguuucagag guuccguaua auuucuaucg uggccaauuu acggauuucc gaaacaaaag     1020

gcgagaaccg accgcugacc gcugacgaga gaaggcacgu agugacguuu cucacugaag     1080

auucacaggc cgaccuuacc ugggucgaug uggcagagaa gcuggggguu caucgccggg     1140

acuugagagg aacagccgug cacacugaug acggugaaag gagcgcugcg cgcccuccca     1200

ucgaugcaac cgaccgaauc augcggcaga caaaaauuag uucucuaaag accugguggg     1260

aggaagccga uuccgagcaa cguggcgcua ugauaagaua ucuguacgag gacccaacgg     1320

acagcgaaug ugccgagauc auugcagaac ucccugagga agaucaggcc aaacuggacu     1380

cgcuucaucu gcccgcuggg agggccgcau auucucgcga guccuugacu gcgcucucag     1440

aucacaugcu ggcuacaacc gacgauuuac acgaggcccg gaagagacug uucggagucg     1500

acgauaguug ggcaccaccc gccgaagcua ucaaugcccc ugugggcaac ccaagcguag     1560

acaggacucu aaagauaguu ggucgauacc ucuccgcagu ggagucuaug ugggggacac     1620

cggaagucau ucauguggag cacguccgcg auggcuuuac cagcgagcgg auggcugacg     1680

aaagagauaa agccaauagg cgucgcuaua acgacaauca ggaggcgaug aagaaaaucc     1740

aacgggauua cggaaaggaa ggcuacaucu caagagggga cauugugagg cuggacgccc     1800

uugaguugca gggaugcgcu ugucuguauu gcgguacgac caucggcuac cauacauguc     1860

agcucgauca cauuguuccc caggcagggc cuggaaguaa caaucgacgg ggcaaccugg     1920

uggccguaug cgaacgcugu aauagaucca aaagcaacac uccauucgcu gucugggccc     1980

aaaagugcgg uauaccccac guggggguua aggaggcaau cggcagggug cggggauggc     2040

gaaaacagac cccuaacaca ucuucagagg accuuacuag acugaagaaa gaagucauug     2100

cgcgcuugag gcguacccag gaggauccag aaaucgacga gcggaguaug gaaagcgugg     2160

ccuggauggc uaaugagcuc caucacagaa ucgccgcagc uuaucccgag acaaccguaa     2220

ugguguacag gggcuccauu acugccgcag cccgcaaggc ugcggggaua gauucucgaa     2280

ucaaccugau uggagaaaaa gguagaaagg accggaucga uaggcgccau cacgccguug     2340

acgcaucggu cguggcuuua auggaggcca gcguggccaa gacgcuggcu gaacguuccu     2400

cacuaagagg cgagcagcgg cucacaggga aagaacaaac cuggaagcag uauacuggaa     2460

guaccgucgg cgcaagggag cauuuugaga uguggcgcgg ucacaugcug caccuuacag     2520

aauuguucaa ugagcgacug gccgaagaua aaguuuacgu gacccagaac auuagacucc     2580

ggcugucuga cgggaaugcg cauacuguga acccgagcaa guuaguaucc cacaggcugg     2640

gcgauggacu uacaguccag caaaucgacc gcgcuuguac gccugccuug uggugcgcac     2700

ucaccagaga gaaagauuuu gacgagaaga auggccugcc agccagggaa gaccgggcua     2760

uacgugugca ugggcacgag aucaagucaa gcgauuauau ucagguuuuc ucuaaacgca     2820

agaaaacuga cuccgaucga gacgaaacac ccuucggagc aaucgccgug agaggcgguu     2880

uugucgagau ugggccuagu auccaucacg cgaggauaua ccggguggaa ggaaagaaac     2940

ccguuuacgc uaugcuacgc guguucaccc acgaucugcu cagccagaga cauggcgacc     3000

uguuuucugc cguaauucca ccucagucca ucucaaugag gugcgcagag cccaaacuuc     3060

ggaaagccau uacaacuggu aacgcuaccu auuugggcug ggucguggug ggggaugagc     3120

uggaaaucaa ugucgacagu uucacgaagu acgccauagg acgauuuuua gaggauuucc     3180

caaacaccac aagauggcgc aucuguggcu augacacuaa uagcaagcuc acccugaaac     3240

cgauuguucu ugcagcugaa gggcuggaga acccuucguc cgccgugaau gaaaucguag     3300

aguugaaggg auggagggug gcgauuaacg uccucacaaa agugcacccc acuguugugc     3360

ggcgugaugc ccugggcaga ccaagguacu cuagccgcuc aaaucuaccc accaguugga     3420

cgaucgagaa gcggccugca gcuacaaaga aggccgguca agcuaagaaa aagaaauaag     3480

gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc     3540

uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaagaauu                                                  3619


<210>  3559
<211>  4480
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3559
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaac caagccauac agcauuggac uggacauagg uacaaacucc guuggcuggg      180

ccgugacuac ggauaauuau aaaguaccgu cuaagaagau gaaagugcuc gggaacaccu      240

caaagaaaua caucaagaaa aaucuucugg gagucuuguu auucgacagu ggcauuacag      300

cggaggguag gcggcugaag cgcacugcuc gacguagaua uaccaggcgg cgcaacagaa      360

uccuauaccu ccaggaaauu uuuagcacag agauggccac ccuggaugac gcauucuuuc      420

aaaggcuuga ugacuccuuc cuggugcccg augacaagcg ggauucuaaa uauccuaucu      480

uugggaauuu gguugaagag aaggccuacc augacgaguu cccaacuaua uaucaccucc      540

gcaaauaccu ggcugauucg acaaagaaag cagaccugcg acucguguac uuagcccugg      600

cucauaugau caaguauaga ggacacuuuc uuauugaggg cgaguucaac ucaaagaaua      660

acgauaucca gaagaauuuu caggacuucu uggacaccua caacgccauu uuugaaagcg      720

aucugagucu agaaaauucc aagcagcucg aagagaucgu caaagacaag auaucuaaac      780

uggagaagaa agauaggauu cuuaagcugu uccccggcga aaagaacagc gggaucuuuu      840

ccgaguucuu gaaacucauu gugggaaauc aagcggacuu ccggaagugc uuuaaccugg      900

augaaaaagc aucauuacau uucaguaagg agagcuauga cgaagaucug gagacgcucc      960

uugguuacau cggcgacgau uauucugacg uauuucugaa agccaagaag uuguacgaug     1020

cuauccuacu guccggguuc cucacuguua ccgacaauga gacagaagcc ccucugucaa     1080

gcgcaaugau uaaacguuau aacgagcaca aggaagaccu ugcucuguug aaagaguaca     1140

uacgcaacau cagucucaag acuuacaaug aaguguuuaa agaugacacc aagaacggau     1200

augccggcua cauugauggu aagacaaauc aggaggacuu cuaugucuac cugaaaaagu     1260

uacuggcgga guuugaaggg gccgauuauu uccuagagaa aaucgacaga gaagauuuuc     1320

ucaggaagca gcggaccuuc gacaacggau cuauaccaua ccagauucac cuucaagaga     1380

ugcgagcaau ccuugauaaa caggcuaagu uuuaucccuu cuuggccaaa aauaaggaac     1440

gcaucgagaa gauucugacg uuuagaaucc cuuacuacgu gggcccacuc gcuaggggca     1500

acuccgacuu cgccuggagc auucggaaac gcaaugagaa gauaacuccg uggaacuucg     1560

aagaugugau cgacaaagag ucgucugcag aagccuuuau uaauagaaug acauccuucg     1620

aucuguaucu ucccgaggag aagguccugc cuaaacauuc auugcucuac gaaaccuuua     1680

acguuuauaa ugagcugacu aaggugaggu ucaucgcuga aaguaugcgu gacuaccagu     1740

uuuuagacag caagcagaaa aaggauaucg uacggcugua uuucaaagac aagcgaaaag     1800

ugacagauaa ggacauuaua gaguaccuac acgcgaucua uggguacgau ggaauugaac     1860

ucaaggguau cgagaaacaa uuuaacuccu cucugagcac cuaccaugac cuuuugaaua     1920

uuaucaacga uaaggaguuc cuggacgauu caaguaauga agcaauaauc gaggaaauua     1980

uccacacgcu caccauuuuu gaggacagag aaaugaucaa acagaggcug uccaaguucg     2040

agaacauauu ugauaaaagc gucuuaaaga agcugucucg ccggcacuau acaggcuggg     2100

ggaaacuuuc agccaaguug auuaacggaa ucagagacga gaaaaguggc aauacuauuc     2160

ucgauuaccu gaucgacgac gguauaagca acaggaauuu caugcagcua auccaugaug     2220

acgcucuguc cuuuaagaaa aagauucaaa aagcccagau cauuggggau gaagacaagg     2280

gcaacaucaa ggaggugguu aaaucucucc caggaucgcc cgcaauaaag aaaggcauuc     2340

ugcagagcau caagaucgug gaugaacuug ucaaagugau gggagggcgc aagccugagu     2400

ccauuguagu ugaaauggcc cgagagaauc aguauaccaa ccaagguaag ucaaauaguc     2460

agcagcgguu gaaacgucug gagaagucuu uaaaagaacu cggcagcaag auccugaaag     2520

agaacauucc agcuaagcug uccaagauag acaauaacgc ccugcagaau gauagauugu     2580

accucuauua ccugcaaaac gggaaagaca uguauacagg agaugaccua gauaucgaca     2640

ggcugucaaa uuacgacauu gaucacauca uuccccaggc auuccucaag gacaacagca     2700

ucgauaauaa agugcugguc ucuuccgcga guaaccgcgg caagagcgac gaugugccgu     2760

cucuugaagu cgugaaaaag cggaagacuu ucugguacca guugcugaaa uccaaguuaa     2820

uaucacagag aaaauuugac aaccucacca aggcugagag gggugggcug aguccugagg     2880

auaaagccgg cuucauccaa cgacagcuug uugaaacacg ccagauuacc aagcaugugg     2940

cacggcugcu agacgagaag uuuaauaaca aaaaggauga aaauaacaga gccguaagga     3000

cugucaaaau cauuacgcuc aagagcacac uggugucgca guuccguaaa gacuuugagu     3060

uguauaaggu ucgcgaaauc aaugauuucc accaugcuca cgacgccuac cugaacgcag     3120

uggucgcuuc cgccuuacuc aaaaaguauc ccaagcugga gccagaguuu guguacggag     3180

auuauccuaa auacaauucu uuccgggaaa gaaagagcgc gaccgagaaa guauauuuuu     3240

acucaaacau aaugaauauu uucaagaaaa guaucucccu ugccgacggc agggugaucg     3300

aacgaccccu gauugagguu aacgaagaga cuggggaguc ugucuggaau aaggaaagcg     3360

acuuggcuac cgugcggcgc gugcucuccu acccacaagu caacguugug aagaaagugg     3420

aggaacagaa ucacggacug gauagaggua agcccaaagg ccuauuuaac gcaaaucugu     3480

caagcaagcc uaaaccaaac aguaacgaga aucuuguagg ggccaaggaa uaucucgacc     3540

cgaagaaaua cggaggcuau gcugguauau cuaacuccuu cacaguccug gugaagggga     3600

ccaucgagaa aggcgccaag aaaaagauua cuaauguuuu ggaguuccag ggaaucagca     3660

uucuggauag gaucaacuac cggaaggaca aauuaaauuu ucuccuggaa aagggcuaua     3720

aagauauaga gcuuaucauu gaacugccca aguacucguu guucgagcuc ucagacgggu     3780

cucgaagaau gcuggcaagu auccuaucca caaacaauaa acgcggagag auucauaagg     3840

gcaaccaaau cuuucugagc cagaaauucg ugaaacuucu cuaucacgcg aagaggauau     3900

cuaauacgau uaacgaaaau caucguaaau acgucgagaa ccacaagaaa gaauuugagg     3960

aacuguucua cuauaucuug gaguuuaaug agaacuacgu gggugccaag aaaaauggga     4020

aguuacugaa cagcgcuuuc caguccuggc agaaucacuc aaucgaugaa cucuguagua     4080

gcuuuauugg accuaccggc ucugagcgga agggucuguu cgaacuuacu uccagaggcu     4140

cagccgcaga cuuugaguuc cuggggguua aaaucccaag guaucgcgau uacacaccca     4200

guagcuugcu aaaggacgcu acccugauac aucaauccgu gacaggacuc uaugaaacuc     4260

gaauugaucu ggccaaacuu ggcgagggga agagaccugc agccaccaaa aaggcuggac     4320

aggcgaagaa aaagaaauaa ggacuagucc cuguucccag agcccacuuu uuuuucuuuu     4380

uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                           4480


<210>  3560
<211>  3562
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3560
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc cgcguucaag ccaaacagca uuaauuacau acugggacuc gacaucggua      180

uugcuuccgu uggcugggcc augguggaga ucgaugaaga ggaaaacccg auuaggcuua      240

ucgaccuggg gguacgggug uuugagcgcg cagaaguccc caaaaccgga gauucuuugg      300

ccauggcucg acguuuagca agaucaguga ggcggcugac acgcagaagg gcccaucggc      360

uacuccgcac ucgaagacug cuuaagaggg agggcguucu gcaggcugcc aauuucgacg      420

agaacgguuu gauaaagagu cucccuaaua cgccauggca acugcgggcg gcagcccugg      480

aucguaaacu cacccccuua gaauggagcg cugugcugcu ucacuugauc aagcaucgcg      540

gguaucuguc ccagaggaaa aacgagggag aaacagccga caaggagcua ggcgcacucc      600

ugaaaggcgu cgcugggaau gcccacgcgc uucagacugg agauuuuagg accccugccg      660

agcuggcauu gaacaaguuc gaaaaggagu cuggucauau ucggaaucag cgaucggacu      720

acucacacac auuuagccgc aaagaucucc aagcugaacu gaucuuacug uucgagaagc      780

agaaagaauu uggcaaccca cacgugagug ggggacucaa ggagggcauu gagacccuuc      840

ugaugacuca gagacccgcc uuguccggug acgcuguaca gaaaaugcua gggcauugca      900

cauucgaacc ugccgagcca aaggcagcca aaaauaccua uacggcugaa agguuuaucu      960

ggcugacuaa gcucaacaau cugcggauac uugagcaagg aucugaacgc ccgcugaccg     1020

auacagagag agcgacuuug auggacgagc ccuacagaaa gagcaaacuc accuaugcac     1080

aggcccguaa gcuguuaggc cuggaagaua cagcuuucuu uaaaggccua cgguacggga     1140

aggacaacgc cgaggcaucc acccucaugg aaaugaaagc cuaucacgcu auuucacgag     1200

cccuggagaa ggaaggacuu aaggacaaaa agaguccuuu gaaucugagc ccagagcucc     1260

aggaugagau cgguacggca uucucucugu uuaaaacuga cgaagauauu acaggcagac     1320

uuaaggacag gauccagccc gagauccugg aagcguugcu caaacauauu uccuucgaua     1380

aguucguuca aauaucacug aaggcuuuac gccggaucgu gccucugaug gagcagggga     1440

aaagauacga cgaggccugu gcagaaauuu acggagauca cuauggcaag aaaaacaccg     1500

aggaaaagau cuaccuacca cccauaccgg ccgacgagau uaggaacccu gucgugcucc     1560

gcgcucugag ccaggcccga aaagugauca auggugucgu ucggcguuau gggagucccg     1620

caagaaucca cauugaaacu gcuagggagg ugggcaaguc uuuuaaggau cgcaaagaga     1680

ucgaaaagcg gcaagaggaa aacagaaaag acagggagaa ggccgcggcc aaauuccgag     1740

aauacuuucc aaauuucgua ggagagccua aguccaaaga uauucuuaag uugcgccugu     1800

augagcagca gcauggcaag ugccucuaca gcgggaaaga aauaaaccug ggacgguuaa     1860

augagaaggg uuauguggaa aucgaccacg cucugcccuu uucgagaaca ugggacgauu     1920

cuuucaacaa uaaaguccuu guguugggcu ccgagaacca gaauaagggg aaccaaaccc     1980

cauacgaaua cuuuaaugga aaagacaacu caagggagug gcaggaguuc aaggcacgug     2040

uugaaacgag ucgcuuuccc cggagcaaga aacagaggau ucuccugcag aaguucgaug     2100

aggacggcuu uaaagaaaga aaucuaaacg auacccgaua ugugaaucgg uuccuguguc     2160

aauuugucgc cgaccgcaug agacucacag guaaggggaa aaagagggug uucgcuucca     2220

acggccagau cacuaaccug cuucggggau ucuggggcuu gcgaaaggua agagccgaga     2280

augaucgcca ucacgcacug gacgcgguug uggucgccug cucuaccgug gcuaugcagc     2340

agaaaaucac aagguuuguc cguuacaagg agaugaacgc cuucgauggg aaaacuauug     2400

acaaggaaac cggagaggug uuacaucaga aaacacacuu uccucagcca ugggaguucu     2460

uugcacagga aguuaugaua cggguguucg guaagccgga uggcaagccc gaauuugagg     2520

aggcugacac ccucgaaaaa cugagaacuc uucuggccga gaaguugagc ucaaggccug     2580

aagcaguaca cgaguauguc acgccacucu ucgugagucg cgcccccaau cgaaaaaugu     2640

ccgggcaggg acauauggaa acaguuaaga gcgcuaaaag acuggaugag ggcgugucug     2700

uccuacgggu gccucugacc caacucaagc ugaaagaccu ugagaagaug guaaacaggg     2760

aacgcgagcc aaaguuguac gaagcgcuga aagcccguuu agaggcacac aaggacgauc     2820

ccgcuaaagc cuuugccgaa ccguucuaua aguacgacaa agcugguaau agaacucaac     2880

aggugaaggc aguucggguc gagcaagugc agaagaccgg gguguggguc aggaaccaua     2940

auggcaucgc cgauaacgcg acaaugguuc gcguggacgu guuugagaaa ggagauaagu     3000

auuaccucgu accuauuuac ucauggcagg ucgcuaaagg cauccugcca gaccgagccg     3060

ugguucaggg gaaggaugaa gaggacuggc aacuuauuga ugacaguuuc aauuuuaaau     3120

ucagccugca ccccaacgau cuaguggaag ucaucaccaa gaaggcaaga auguucggau     3180

auuuugccuc cugucaccgg ggcacuggua auauaaacau caggauucau gaccucgauc     3240

acaaaaucgg gaagaauggc auucuggagg gaaucggugu gaaaacagcu uugucuuucc     3300

agaaguacca gauagacgag cugggcaaag aaauucgccc uugcagauua aagaagaggc     3360

ccccaguucg gaaacguccu gcagccacga agaaagcggg gcaggcuaag aaaaagaagu     3420

aaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu     3480

gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaagaa uu                                              3562


<210>  3561
<211>  4318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3561
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaaaccauac accauuggac uggacauagg uacaaacagc guuggcuggg      180

ccgugcucac ugaucaguau gaccuuguaa agaggaagau gaaaaucgcg ggggauuccg      240

agaagaaaca aauuaagaaa aauuucuggg gagugcggcu guuugacgaa ggccagacgg      300

cugccgaucg ccgaauggca cguaccgcca gaaggcggau cgagcgcaga aggaaccgga      360

uuucuuacuu gcaggguauc uucgcugaag agaugucaaa gacagacgca aacuuuuucu      420

gccgcuuaag ugauagcuuu uaugucgaca augaaaagcg aaauuccaga cauccguucu      480

uugccacuau agaggaggaa guggaguacc acaaaaacua ucccaccauc uaccaucuga      540

gggaggagcu aguuaauucu ucggaaaagg cugaucuccg gcugguguau cuugcccugg      600

cgcacauuau caaauaccgu gggaacuucu ugauugaagg agcacucgac acacagaaua      660

ccucagucga uggcaucuac aagcaauuua uacagacuua uaaccaggug uucgccagcg      720

gcauugagga cgggagucug aaaaagcugg aagauaauaa agacguagcu aagauccucg      780

uugagaaggu gacacgcaaa gaaaaguuag agaggauucu gaaacuuuac ccuggagaga      840

aguccgccgg uauguuugca caguucaucu cuuugaucgu cggcagcaaa gggaacuuuc      900

aaaagccauu cgaccugauu gaaaaguccg auauagagug ugcuaaagac ucauaugaag      960

aggaucuaga aagucuccug gcccuuaucg gagacgagua cgcggagcug uucguggccg     1020

caaagaaugc uuauagcgcc guggucuugu cuuccauuau caccguugcu gaaacggaga     1080

cuaacgccaa acucucagca agcaugauag aaagguuuga uacccaugag gaagaccugg     1140

gcgaguuaaa ggccuucauu aaacugcacc uccccaagca cuacgaggaa aucuuuagua     1200

auacagagaa gcaugguuau gcuggguaca ucgauggaaa aacuaagcag gcggacuucu     1260

acaaauauau gaagaugacc cuugaaaaca uugagggcgc agauuacuuu aucgccaaaa     1320

uugagaagga aaacuuccug cggaagcagc gaacauuuga caauggcgcu auaccucacc     1380

aguugcaucu agaggaacug gaggccaucc uccaccaaca ggcaaaauau uacccauucc     1440

ugaaggaaaa cuaugauaaa auuaagucuc uugugaccuu ucgcaucccc uacuucguag     1500

ggccucuggc caauggacag uccgaguuug cuugguugac gagaaaagcc gacggugaga     1560

ucaggccaug gaacauugaa gagaaggugg acuucggcaa aagcgcaguc gauuuuauag     1620

aaaagaugac uaauaaggac acauaucucc cgaaagagaa cgugcugccc aagcacucgu     1680

uaugcuacca aaaauaccug guuuauaaug aacuaaccaa ggugcgguac aucaacgauc     1740

aggggaaaac uucuuauuuc uccggacagg agaaggagca gauuuucaau gaccucuuua     1800

agcaaaaacg caaggucaaa aagaaagauc uggaacuuuu cuugagaaac augucacaug     1860

uggagagucc uacaaucgaa ggccuggagg acagcuuuaa uuccucuuac agcaccuauc     1920

acgaucuccu gaagguaggu auuaagcagg aaauccuuga caacccaguu aauacggaga     1980

ugcuggagaa cauagugaaa aucuugaccg ucuucgaaga uaagaggaug auuaaagagc     2040

agcuccagca auuuucagac gugcuggaug gggucguguu aaagaaacug gaacgucggc     2100

auuacacagg cuggggacga cuaagugcga agcuccugau gggcaucaga gacaagcagu     2160

cccaccuuac uauuuuggau uaucugauga augacgacgg gcucaacagg aaccugaugc     2220

aguuaaucaa ugauagcaac cugucuuuca aaucaauaau ugagaaggag cagguuacca     2280

cagcugacaa agauauccaa aguauugugg ccgaccuugc aggaagcccc gccaucaaga     2340

aagguauauu gcagucccuc aagaucguag augaacuggu cucugugaug ggcuacccuc     2400

cacagacuau uguuguggag auggcucgcg aaaaucagac cacagggaaa ggaaagaaca     2460

auucgcggcc cagauacaag agccuagaga aagccaucaa ggaauuuggc ucccaaauuc     2520

ugaaagagca uccgaccgac aaccaggagc ucaggaauaa ccgccuguau cuuuacuauu     2580

ugcagaaugg uaaggauaug uacacugggc aagaccugga uauccacaac uuaucaaauu     2640

augacauaga ucacauuguc ccucagaguu ucaucacgga caacucuauc gauaaucucg     2700

ugcugacaag cuccgcaggc aaccgagaaa aaggagacga cguaccccca cuugagauug     2760

ugcggaagcg uaaggucuuu ugggaaaaac uguaccaggg caauuugaug ucaaagagaa     2820

aauucgauua ucucaccaag gcugagagag ggggacugac ugaagccgac aaagcgcgcu     2880

uuauccaucg gcagcuaguu gagaccagac aaauuacaaa gaacguggcc aacauacugc     2940

accagagguu caauuacgag aaggaugacc augguaacac caugaaacag gugcgaaucg     3000

ucacucucaa gagcgcucug guuucucagu uucgcaaaca auuccagcuu uacaaggugc     3060

gggaugugaa ugacuaucac cacgcacaug augccuacuu gaacggcgua gucgcuaaua     3120

cacuguuaaa aguguauccu cagcucgaac ccgaguucgu uuacggggac uaucaccagu     3180

uugauugguu caaggccaac aaggcaacgg cgaaaaagca auuuuacacc aauauuaugc     3240

uguucuuugc ccagaaagac agaaucauug augaaaacgg agagauccuu ugggacaaga     3300

aauaucugga cacugugaag aaggucaugu ccuacaggca gaugaauaua gugaaaaaga     3360

cagaaaucca aaaaggcgag uucaguaagg cuaccauuaa accaaagggu aacagcucua     3420

aacuaauccc ccguaagaca aauugggauc cuaugaagua cgggggccuc gacuccccaa     3480

acauggccua ugcaguugug auugaguacg cuaaaggaaa gaauaaacug guauuugaaa     3540

agaaaaucau acgcgucacu auuauggagc ggaaggccuu cgaaaaggau gagaaagcau     3600

uuuuggagga acagggcuau agacagccga aggugcuggc caaauuaccc aaguacaccc     3660

ucuaugagug ugaagagggg aggcgacgga ugcuggcuuc agcgaacgaa gcccagaaag     3720

gaaaucaaca ggugcuuccu aaccaucugg ucacguugcu ccaccaugca gcuaacugcg     3780

agguuaguga cggcaagagc cuggauuaca ucgagucgaa ucgcgaaaug uucgccgagc     3840

uacuggccca cguguccgaa uuugcuaaga gauauacccu ugcagaggcc aaccucaaua     3900

aaaucaacca gcuguucgaa cagaauaagg agggugacau uaaagcgaua gcucaaucuu     3960

ucguagauuu gauggccuuu aacgcaaugg gggccccagc uagcuucaag uuuuucgaga     4020

caacuaucga aaggaaacgg uacaauaacc ugaaggaguu acucaauuca accauuaucu     4080

accagaguau uacaggacug uaugaauccc gaaagagacu ugacgauaaa cgccccgcag     4140

ccacuaagaa agcgggccag gcuaagaaaa agaaauaagg acuagucccu guucccagag     4200

cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu       4318


<210>  3562
<211>  4351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3562
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaaaccauac agcauuggac uggacauagg uaccaacucc guuggcuggg      180

ccgugguaac agaugacuau aaggugccgg cgaagaaaau gaagguccuc gggaauacug      240

auaaaucuca uaucgagaag aaccuucugg gagcuuuguu auucgacuca ggcaauacgg      300

ccgaagauag gcggcugaaa cgcaccgcac gacguagaua cacaaggcgg cgcaaccgga      360

uucuauaucu ccaggagauc uuuagugaag agauggguaa gguggacgau agcuucuuuc      420

acaggcugga agacuccuuc cuuguuacug aggauaagag aggggagcgc caucccauuu      480

uuggaaaucu ggaagaggaa gugaaauacc acgagaacuu cccuaccauc uaucauuugc      540

gacaauaccu cgccgacaau ccagagaagg ucgaucugag acugguguau cucgcuuuag      600

cacacauaau caaauuuagg ggccacuucc ugauugaagg caaguuugac acacggaaca      660

augauguaca gcgucuuuuc caggaguuuu uggccguuua cgacaacacc uucgaaaauu      720

cuucgcugca ggagcaaaac gugcaggucg aagagauccu aacugacaaa auuucaaaga      780

gcgcuaaaaa ggaucgcgug cucaagcugu uucccaauga gaaaaguaac gggagauucg      840

ccgaguuccu uaagcugauc gugggaaauc aggcggacuu uaaaaagcau uucgaguugg      900

aagagaaagc accucuccag uuuuccaagg auacauacga agaggagcug gaagucuuac      960

uggcccaaau aggugacaac uaugcugaac ucuuccuuuc ugccaagaaa cuguacgaua     1020

gcauuuugcu auccggcauc cugaccguua cggacguggg gacuaaggca ccacucucag     1080

cuaguaugau ucagagguau aacgaacacc agauggaucu ggcccagcuu aaacaauuua     1140

uccggcagaa gcugagcgac aaauacaaug agguauucuc ugaugugucc aaggacggau     1200

augcgggcua caucgauggu aagaccaacc aggaagccuu uuauaaauac uugaaggggc     1260

uccugaauaa aauugaggga ucaggcuacu ucuuagacaa gauagagcga gaagacuuuc     1320

ugcgcaaaca aagaacauuc gauaacggca gcauccccca ucagauucac cuacaggaga     1380

ugagggcaau cauacggcgc caggcugaau uuuauccuuu ccucgccgac aaucaagaua     1440

gaauugagaa gcugcuuacu uuuaggaucc cauacuaugu cgggccguug gcucguggaa     1500

agagugacuu cgccuggcug ucucggaaau ccgcagauaa gaucaccccc uggaacuucg     1560

acgagauugu ggauaaagaa agcucggccg aggcuuuuau caaucgaaug acaaacuacg     1620

accucuaucu gccuaaucag aagguucuuc caaaacacuc ucuguuguac gaaaaguuca     1680

ccguguauaa cgagcucacg aaagucaagu acaagacuga acaggguaaa acagcguuuu     1740

ucgaugcaaa uaugaagcag gagauuuuug acggcguguu caaaguauac agaaagguua     1800

ccaaagauaa gcugauggac uuuuuagaga aggaguucga ugaauuuagg auaguggacc     1860

ugacugggcu agacaaagaa aacaaggucu ucaaugccuc cuauggaaca uaccaugauc     1920

ucugcaaaau ccuggacaag gauuuucuug acaacaguaa aaaugagaag auuuuggaag     1980

auaucgugcu gacccucacg cuguucgagg accgcgagau gauccggaag agauuagaaa     2040

acuauucaga ucugcuuacc aaagagcaag ucaagaaauu ggaaaggcgc cacuacacag     2100

gcuggggucg acucagcgcu gagcugauuc augggauacg gaacaaggaa ucccguaaaa     2160

cuauccuaga cuaucugauu gaugacggca auucuaacag aaauuuuaug cagcucauca     2220

acgaugacgc ccugagcuuc aaggaggaga uugcaaaggc ccaggugauc ggagaaaccg     2280

auaaucuuaa ccagguugug ucagacauag cuggcagucc cgccaucaaa aaggggauuu     2340

ugcaaucccu gaaaaucgua gacgaguuag ucaagauuau gggacaccag ccugaaaaua     2400

ucgugguuga gauggcaagg gagaaccagu ucacaaauca gggucgccgg aacagccaac     2460

agagacucaa aggccugacu gauucuauaa aggaauuugg gucacagauu cuuaaagagc     2520

auccagugga aaauagucaa cugcagaacg acagguuguu ccucuacuau cugcagaaug     2580

gacgagauau guacaccggc gaggaacuag acaucgauua ccugagccag uaugacauug     2640

aucacaucau accccaagcg uuuaucaagg acaacuccau ugauaaucgc guccucacau     2700

cuucgaagga gaaccggggu aaaagcgacg augugccguc caaggacgua gugagaaaaa     2760

ugaagucaua cuggaguaaa cugcuuucug cuaaguugau cacccagagg aaguucgaca     2820

accugacuaa agccgagcgu gggggcuuaa cggaugacga uaaggcagga uuuauuaaac     2880

gccagcucgu ugaaacacgg cagaucacca agcacgucgc cagaauacug gacgagaggu     2940

ucaauacuga aaccgaugag aacaauaaaa agauucgaca agugaagauc gugacacuua     3000

aaagcaaccu ggucuccaau uuucggaagg aguucgaacu auauaaaguu cgcgagauca     3060

acgacuacca ucacgcucau gaugccuauc ucaaugcagu gauuggcaag gcucuguugg     3120

ggguguaccc ucagcuggaa cccgaguuug uauauggaga cuacccacac uuccacgguc     3180

auaaagaaaa caaggccacc gcgaagaaau uuuucuacuc aaauaucaug aacuucuuua     3240

agaaagauga cgucagaacu gauaagaaug gcgagauuau auggaaaaag gacgaacaca     3300

ucagcaacau uaagaaagug uuaucuuauc cucagguuaa uaucgugaag aaagucgagg     3360

agcagacagg gggauucucc aaggaaagua uucuccccaa aggcaacagc gauaagcuga     3420

ucccaaggaa aacgaagaag uuuuacuggg acaccaaaaa guaugguggg uucgacucuc     3480

ccauaguggc cuacuccauc cuuguuauug cugauaucga gaaaggcaag aguaaaaagc     3540

ugaagacugu gaaagcauug guaggaguca caauuaugga aaagaugacc uuugagcggg     3600

acccuguggc cuuccucgag cgaaaaggcu auagaaaugu gcaagaagag aacaucauaa     3660

agcugccaaa auacucacua uuuaagcugg aaaacgggcg caagaggcuu cucgcuagcg     3720

cccgugagcu gcagaaagga aaugaaauug ucuugccgaa ccaucugggc acauuacucu     3780

aucacgcaaa gaauauccau aaaguugaug agcccaagca ccuggacuac guggauaaac     3840

acaaggacga guucaaggaa cuucuggaug uagugucgaa cuuuuccaaa aaguacacuu     3900

uggcggaggg uaaucucgag aaaaucaagg aacuguaugc ccagaacaau ggggaagacc     3960

uaaaagagcu ggcuucuagc uucauuaacc uucucaccuu uacggccaua ggagcuccug     4020

caaccuucaa guuuuucgau aaaaauaucg accggaagag auacacauca acuaccgaga     4080

uucugaacgc cacauugauc caucaaagua uuacuggcuu auaugaaacc aggaucgauc     4140

ugaauaagcu cgguggcgac aaacgcccag cagccacgaa gaaagcuggg caggcgaaga     4200

aaaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu uuuugaaaua     4260

aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                                    4351


<210>  3563
<211>  3484
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3563
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaca gacaacaaac cugagcuaca uucucggacu ugaccugggu auagccuccg      180

uuggcugggc ggugguagag aucaaugaaa acgaggaucc aauuggguug aucgacgugg      240

gagucaggau uuucgaacgg gcugaggugc cgaagacugg cgaaucuuua gcccugucac      300

gccgacuagc acguaguacg agaaggcuca uccggcgcag agcccauagg cugcuucugg      360

cuaaacgguu uuugaagcgc gaggguauac ucagcaccau cgaucuggag aaggggcugc      420

ccaaucaagc augggaacuc cgaguugccg gauuagagag aaggcugucc gcuauugaau      480

ggggcgccgu gcuuuugcac cugaucaaac aucggggcua ucuaucuaag cguaaaaacg      540

agucgcagac aaauaacaag gagcucgggg cgcugcuuuc aggagucgca cagaaucacc      600

agcuguugca aagcgacgau uaccgcacuc cugccgaacu cgcucugaaa aaguucgcca      660

aggaggaagg ucauauuaga aaccagaggg gcgcauauac ccacacauuu aaucgguuag      720

accugcucgc ugagcuuaac cuguuguucg cccagcagca ccaauuuggg aauccacauu      780

gcaaagaaca cauccagcag uacaugaccg agcuacugau guggcagaag cccgcgcuca      840

guggagaggc cauacugaaa augcuuggca aguguacuca ugagaaaaac gaguucaagg      900

cagcuaaaca cacauauucc gccgaacgau uuguguggcu gaccaaguug aauaaccucc      960

gcauucugga ggauggugcu gaaagagccu uaaaugagga ggaaaggcaa cugcuaauca     1020

accacccuua cgagaagucu aaacucacgu augcacaggu acggaagcug cuuggguuga     1080

gcgaacaggc cauuuucaaa caucugcgcu acuccaagga gaacgcugaa ucagcgacuu     1140

uuauggagcu caaagcaugg cacgccauca gaaaggcucu ggagaaucaa ggacuuaagg     1200

acaccuggca ggaucuggcc aaaaagccag acuugcucga ugaaaucggc acagcauuca     1260

gucuguacaa aacugacgag gauauucagc aguauuuaac caacaagguu cccaauagcg     1320

ugauaaacgc ccugcuaguc ucucucaauu uugacaaauu caucgaacug ucccuuaagu     1380

cauugaggaa gauucugccu cucauggagc aaggcaaacg uuacgaccag gcuugccggg     1440

agaucuaugg gcaucacuac ggagaagcca accagaagac aagccagcug uuaccagcaa     1500

uaccggcgca agagauucga aaucccgugg ugcugagaac ccuuagucag gcuaggaaag     1560

ucaucaacgc caucauucgc caguaugguu cuccugcacg gguucauauc gaaacgggca     1620

gagaguuggg gaaguccuuu aaagaaaggc gcgagauuca gaagcaacag gaggauaauc     1680

gaacuaagcg ggaaagcgcc gugcagaaau ucaaggaacu cuucucggac uuuucuuccg     1740

aaccaaaauc aaaggauaua cugaaauucc gucuauacga gcagcaacac ggaaaguguc     1800

uguauagugg caaagaaauc aacauucaca gacucaauga gaaggguuac guagagaucg     1860

accaugcucu gcccuuuagc aggacauggg augacuccuu caacaauaag gugcuugucu     1920

uggccucuga aaaccagaau aaagggaacc agaccccuua cgaguggcug caaggcaaga     1980

ucaacagcga acgcuggaaa aauuuugugg cauuaguucu cggaucacag ugcagugcug     2040

ccaagaaaca gcggcugcuu acucagguga uugaugacaa caaguucaua gauagaaauc     2100

ugaacgacac aagguauauc gcgcgauuuu uguccaauua cauucaagag aaccuccugc     2160

uagucggcaa gaauaaaaag aacguguuca ccccaaaugg gcagaucacg gcccugcucc     2220

gcagccggug gggacugauu aaagcuagag aaaacaauaa caggcaccau gcacuugaug     2280

ccaucguagu ugcuugugcc acccccucua ugcagcagaa gauaacacgu uuuauccgcu     2340

ucaaagaggu gcacccguau aagauugaga aucgguacga aauggucgac caagagucag     2400

gugaaaucau uaguccucac uuucccgagc caugggcaua uuucagacag gaggugaaca     2460

ucagggucuu ugacaaucau ccugauacug uguugaagga aaugcugccc gaccgaccac     2520

aggcgaacca ccaguucguu caacccuuau ucgugagccg ggccccuacc cgcaaaaugu     2580

ccggccaggg gcauauggag acaauaaagu cugcuaaaag acucgccgaa ggaauuucgg     2640

uacugaggau cccacuuacu cagcugaagc cgaaccuacu cgagaauaug gucaacaaag     2700

aacgggagcc cgcacuguac gcuggcuuga aggcccgacu ggcagaguuu aaucaagauc     2760

cugccaaggc uuucgcgacc ccauuuuaua aacagggugg gcaacaggug aaggccauua     2820

gaguugaaca ggugcagaaa agcggcgucu uagugcgcga gaacaaugga guagcagaca     2880

acgcuuccau cgugaggaca gauguuuuca uaaagaauaa caaauuuuuc cucgucccca     2940

ucuacaccug gcaaguggcc aagggcauuc ugccuaauaa agccaucgug gcucacaaga     3000

acgaagacga gugggaagag auggaugagg gggcaaaguu uaaauucuca cuuuuuccaa     3060

augaccuggu cgaauugaag acuaaaaagg aguacuucuu uggauauuac auuggucucg     3120

aucgugccac gggcaacauc agucugaaag aacaugacgg ggagauaucu aagggaaagg     3180

auggcguuua ucgggugggu gugaaacuag cgcugagcuu cgaaaaguac cagguagacg     3240

agcuugggaa aaauagacag auuugcaggc cccagcagcg ccagccgguc cgaaagagac     3300

cugcugccac caaaaaggca ggccaagcca agaaaaagaa auaaggacua gucccuguuc     3360

ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca gaucuaaaaa     3420

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag     3480

aauu                                                                  3484


<210>  3564
<211>  3562
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3564
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc cgcguucaag ccaaacccga uuaauuacau acugggacuc gacaucggua      180

uugcuagcgu uggcugggcc augguggaga ucgaugaaga cgagaacccc auuugccuua      240

ucgaucuggg gguaagggug uuugaacggg cagagguccc uaaaaccgga gacuccuugg      300

ccauggcucg ccgauuagca cguucuguga gaaggcugac acggcgcaga gcccauaggc      360

uacuccgggc ucgccgacug cuuaagagag aaggcguucu gcaggccgcg gauuucgacg      420

agaaugguuu gauaaaguca cucccaaaca cucccuggca acugagggca gccgcucugg      480

aucggaaacu cacgccuuua gaguggagug ccgugcugcu ucacuugauc aagcaucgug      540

gguaucugag ccagcgcaaa aaugaaggag agaccgcaga caaggaacua ggcgcucucc      600

ugaaaggcgu cgccgauaac gcgcacgccc uucagacagg ggacuuuaga acuccagcag      660

agcuggcuuu gaauaaguuc gagaaggaau ccggacauau uaggaaccag cggggugauu      720

acucucacac cuuuucgcga aaagaccucc aagccgagcu gaucuuacug uucgaaaagc      780

agaaagaguu uggcaauccc cacgugucag ggggacucaa ggaaggcauu gagacacuuc      840

ugaugaccca gcgcccugcu uugagcggug augccguaca gaaaaugcua gggcauugua      900

cuuucgagcc agcagaaccg aaggccgcua aaaacacaua uaccgcggag agauuuaucu      960

ggcugacgaa gcucaauaac cugaggauac uugaacaagg aagugagcgg ccccugacug     1020

acaccgaacg cgcaacauug auggacgagc cuuacagaaa guccaaacuc acuuaugccc     1080

aggcuaggaa gcuguuaggc cuggaggaua ccgccuucuu uaaaggccua cguuacggga     1140

aggacaaugc agaagccucu acacucaugg agaugaaggc uuaucacgcc auuagccggg     1200

cacuggaaaa ggagggacuu aaggauaaaa aguccccauu gaaccuguca cccgaacucc     1260

aggacgagau cgguaccgcg uucagucugu uuaaaacgga ugaggacauu acuggccgac     1320

uuaaggauag aauccagccu gaaauccugg aggcuuugcu caaacauauu agcuucgaca     1380

aguucguuca aauaucucug aaggccuuaa ggcgcaucgu gccacugaug gaacagggga     1440

aacgguacga ugaggcaugc gccgagauuu acggagacca cuauggcaag aaaaauacag     1500

aagagaagau cuaccuaccc ccgauaccug cugaugaaau uagaaacccc gucgugcuca     1560

gggcccuguc ccaggcacgc aaagugauca acggugucgu ucgacgguau gggucaccag     1620

cucguaucca cauugagacc gccagagaag ugggcaagag cuuuaaggac aggaaagaga     1680

ucgagaagcg ccaagaagag aaucggaaag acagagaaaa ggcggccgcu aaauucaggg     1740

aguacuuucc uaacuucgua ggagaaccca agaguaaaga uauucuuaag uugcgacugu     1800

augagcagca gcauggcaag ugucucuacu cugggaaaga gauaaaucug ggacgcuuaa     1860

acgaaaaggg uuauguggag aucgaccacg cacugccauu uucccggacu ugggaugaca     1920

gcuucaauaa caaaguccuu guguugggcu cggaaaauca gaacaagggg aaucaaacac     1980

ccuacgagua cuuuaacgga aaagauaauu cuagagaaug gcaggaguuc aaggccaggg     2040

uugagaccuc ccguuuuccu cgcucaaaga aacagcggau ucuccugcag aaguucgacg     2100

aagauggcuu uaaagagaga aaccuaaaug acacgaggua ugugaaccga uuccugugcc     2160

aauuugucgc ugaucggaug cgccucaccg guaaggggaa aaagagagug uucgccagua     2220

auggccagau cacaaaccug cuuaggggau ucuggggcuu gcggaaggua cgagcagaaa     2280

acgacagaca ucacgcgcug gaugccguug uggucgcuug uagcacugug gccaugcagc     2340

agaaaaucac ccgcuuuguc agguacaagg agaugaaugc auucgacggg aaaacaauug     2400

auaaggagac uggagaagug uuacaucaga aaacccacuu uccacagccg ugggaguucu     2460

uugcucagga aguuaugaua cguguguucg guaagcccga cggcaagccu gaguuugaag     2520

aggccgacac accagagaaa cuccggaccc ugcuugcaga aaagcugucc ucuagacccg     2580

aggccguaca cgaauauguc acuccuuugu ucgugagcag ggcuccaaac cgcaaaaugu     2640

cagggcaggg acauauggag acgguuaaga gugcgaaacg acucgaugaa ggcguguccg     2700

uccugagagu gccccuaaca caacugaagc ucaaagaccu ggagaagaug guaaaucggg     2760

agagggaacc gaaacuuuac gaggccuuga aagcacgccu ggaagcucac aaggaugacc     2820

cugccaaagc cuuugcugag ccauucuaua aguacgauaa agcagguaac cguacccaac     2880

aggugaaggc cguuagaguc gaacaagugc agaagacugg gguguggguc cggaaucaua     2940

acggcaucgc ggacaaugcu accaugguua ggguggaugu guuugagaaa ggagacaagu     3000

auuacuuagu acccauuuac agcuggcagg ucgccaaagg cauccucccu gaucgcgcag     3060

ugguucaggg gaaggacgag gaagauuggc aacugauuga cgauucuuuc aacuuuaaau     3120

ucucacuuca ccccaaugac cugguggagg ucaucacaaa gaaggcccga auguucggau     3180

auuuugcuag uugccacaga ggcaccggua acauaaauau ccggauucau gaccuagauc     3240

acaaaaucgg gaagaacggc auucucgaag gaaucggugu gaaaacugca cugagcuucc     3300

agaaguacca gauagacgag uugggcaaag agauuaggcc augucgccug aagaagagac     3360

cucccguuag gaaacggcca gccgcgacaa agaaagcugg gcaggccaag aaaaagaagu     3420

aaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu     3480

gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaagaa uu                                              3562


<210>  3565
<211>  3568
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3565
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcacccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa guaccacguu ggaauugacg uggguaccuu cagcguaggc cuggccgcga      180

uagaggugga ugacgcuggg augccaauca aaacacucuc ccuugucucu cacauucaug      240

auucaggacu ggacccggau gaaaucaaga gugccgugac uagguuggca agcuccggca      300

uugcccggcg cacgcgacgu uuauauagaa ggaagcggcg cagacugcag caacuagaca      360

aauuuaucca gaggcagggu uggcccguua uagagcucga agauuacucu gacccucugu      420

auccauggaa ggugcgggcu gagcuugcag ccucguacau cgcugaugaa aaagagcgcg      480

gggagaagcu gucagucgcc uugcgacaca uugcgagaca uaggggaugg cggaaccccu      540

augcaaaagu gagcagucuc uaccugccug acggcccauc cgaugccuuc aaggcuaucc      600

gugaagagau uaagcgcgcc ucuggccagc ccguaccuga aaccgcaaca guugggcaaa      660

uggugacucu gugcgagcuc ggaaccuuaa aacugagagg ugagggcggg guccuuagcg      720

cuagguugca gcaguccgac uaugcccggg aaauccagga gauaugucga augcaagaaa      780

uuggacagga gcuguaccgc aagaucauug augugguguu ugcggccgaa ucaccaaaag      840

gcagugcaag cucuagaguc gguaaggacc cgcuacagcc cgggaaaaau agggcucuca      900

aggccuccga cgcuuuccag cgguaccgca ucgccgcacu gaucggaaac cuuagaguua      960

ggguggaugg cgagaaacgu auucugucag uagaggaaaa gaauuuggug uuugaccacc     1020

ucgucaaccu gacaccuaag aaagagccag aaugggugac cauagccgag aucuuaggca     1080

uugaucgggg gcaacugauc ggaacugcua caaugaccga cgauggugaa cgagcgggcg     1140

caagaccccc uacgcacgac acuaauagga gcauaguuaa cagucgcauu gccccacucg     1200

uggauuggug gaagaccgcu ucugcccuug agcagcaugc aauggucaaa gcccugucca     1260

augcugaggu ggacgauuuc gacagccccg aaggggccaa gguacaggca uuuuucgcgg     1320

auuuggacga cgauguucac gcuaaacuag acucgcugca ucucccggug ggacgggccg     1380

cauauucuga ggauacacug gucagacuua cuaggcgcau gcuguccgac ggcguggauu     1440

uguacaccgc ccgacuccaa gaauuuggua ucgagccuuc auggacaccc ccaaccccuc     1500

ggaucgggga acccgucggc aacccagcug uggaccgugu ucugaagacg gugaguagau     1560

gguuagagag cgccacuaag acauggggag cacccgagag gguaauuauc gaacacgucc     1620

gcgagggcuu cgugaccgaa aaacgggcua gagagaugga uggggacaug aggcgacgcg     1680

ccgcgcggaa ugccaagcug uuucaggaga ugcaggaaaa acuaaacguu cagggaaagc     1740

cuuccagagc ugaucucugg agguaucaau cugugcagcg ucagaauugc cagugugcau     1800

acugcgguag cccaauuacu uucucaaaca gugagaugga ccacauaguc ccgcgcgccg     1860

gccaaggguc cacaaauacc cgggaaaacc ugguggcugu augucauaga ugcaaucaga     1920

gcaaaggaaa cacgcccuuu gccaucuggg caaagaacac cucuauugag ggcgugucag     1980

uuaaggaagc ggucgagagg acacgacacu gggugacuga uaccgguaug cggaguacag     2040

acuucaaaaa guucacuaaa gccguggucg agcgcuuuca gagagcuacc auggaugaag     2100

agaucgacgc caggagcaug gaauccguug cauggauggc uaaugagcuu cggucucgag     2160

uggcccagca uuucgcaucg cacgggacaa ccgugagagu auaucgcggc agcuugacug     2220

ccgaagcuag gcgugcgucc ggaaucucag gcaagcugaa auuuuucgac ggggucggaa     2280

agagucggcu cgauagaagg caucacgcca uugacgcagc ugugauagcc uuuacgucug     2340

auuacguugc cgagacacug gcugugcgca gcaaccuuaa gcaaucccag gcacaccgac     2400

aggaggcccc ucaguggaga gaguucaccg guaaagacgc ggaacaucgg gcugccugga     2460

gggucuggug ucaaaagaug gaaaaacugu cagcauugcu cacugaggau cugcgcgacg     2520

aucguguggu ugugaugagc aauguaagau uacggcuggg caacgggucu gcccacaagg     2580

aaaccaucgg aaaacuaucc aaggucaaac ucaguagcca gcugucugug uccgacauug     2640

auaaggcuuc aagugaggca cuuuggugcg ccuugacaag ggagccaggc uuugacccca     2700

aggaaggucu gccugcgaau ccagagcgcc auauccgagu gaacgggacc cacgucuaug     2760

cuggcgauaa uauuggacuc uuccccguua gcgccggcuc gaucgcacug agagggggau     2820

acgccgaauu aggcuccucu uuucaccaug cucgggugua caaaauaacu agcgguaaga     2880

aaccggccuu cgcaaugcug aggguauaua caaucgaccu uuugccuuac cgcaaccagg     2940

aucucuuuuc aguggagcug aagccacaaa cgaugaguau gagacaggcu gagaaaaagc     3000

uaagggacgc ccuggcgacc gggaaugccg aauaucucgg auggcugguc guggacgaug     3060

agcuuguugu ggacacuucc aagauugcaa cagaucaggu caaagcugug gaagccgagu     3120

ugggcaccau ccggcguugg cgcguugacg guuucuuuuc ucccagcaag cugcgauuaa     3180

gaccucucca gauguccaaa gaaggcauua agaaagaguc agcucccgag cugagcaaga     3240

ucauagauag gccagggugg cuuccugcag ugaacaagcu guucagugac ggaaauguca     3300

caguagugcg gcgcgauucu uugggcagag uuaggcucga auccacugcc caucugcccg     3360

ugaccuggaa aguccaaaag cggccagccg cuacgaaaaa ggcggggcag gccaaaaaga     3420

agaaauaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa     3480

uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        3568


<210>  3566
<211>  3268
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3566
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc caggauucug gcguucgaca uaggaaucag cuccauuggu ugggcuuuuu      180

cugagaacga ugaacucaag gacugcggcg uucggaucuu caccaaagug gagaauccaa      240

agacagggga aucacuugcc cugccgcgcc gauuggcacg uagugccaga aagagguuag      300

cucggcgcaa agcaagacug aaccaucuaa agcaccucau ugccaaugag uuuaaacuga      360

acuacgaaga uuaucagagc uucgacgagu cccuugcuaa ggccuacaaa ggaucucuga      420

ucucgcccua ugaguugagg uuucgggcgc ucaaugaacu gcugucaaag caagauuucg      480

cacgcguaau acuccauauc gccaagcgaa gaggcuacga cgauauuaaa aacagcgacg      540

auaaggagaa aggugcuauc uuaaaggcca uuaaacagaa ugaagagaag cuggcaaacu      600

aucagagugu gggggaguac cuuuauaaag aauacuuuca gaaguucaag gaaaauucca      660

aagaauuuac uaacgucagg aauaagaaag agucuuacga acgguguauc gcucagagcu      720

ucuugaagga cgagcugaaa cuaauauuua agaagcaacg ugaguucgga uuuuccuucu      780

cuaaaaaguu cgaagaggaa gugcucagug uugccuuuua uaaacgcgcg cugaaggauu      840

ucagccaccu ugugggcaac ugcucauuuu ucacggacga gaaaagagcc ccuaaaaauu      900

ccccacuggc auuuauguuc gucgcuuuga ccaggauuau caaccuccug aauaacuuaa      960

agaacacaga aggcauucug uacacuaaag augaccucaa ugcccuucug aacgaggugu     1020

ugaagaaugg gacccuaaca uauaaacaga ccaagaaacu gcucggacug ucagacgauu     1080

acgaguuuaa gggugaaaag ggcacuuauu ucaucgaguu uaaaaaguac aaagaguuca     1140

ucaaggcucu uggggagcau aaccugagcc aggacgauuu gaaugaaauu gccaaagaca     1200

uaacacucau caaggaugag auuaaacuga agaaggcauu agccaaauau gaccugaacc     1260

aaaaucagau cgauagucua ucuaagcucg aguuuaaaga ccaccugaac auauccuuca     1320

aggcucuuaa auugguaacu ccccugaugc ucgaaggaaa gaaguacgau gaggcgugua     1380

augaacugaa ccuuaaaguu gcaauuaaug aggacaagaa agauuuucug ccugccuuca     1440

acgagacgua cuauaaggac gaagugacua auccagucgu guugcgggcu aucaaagagu     1500

accgaaaggu gcucaacgcc cuguuaaaga aauauggcaa gguccacaaa aucaauauug     1560

aacuggcacg cgagguuggu aagaaccaua gccagagagc caaaaucgaa aaggagcaga     1620

acgagaauua caaggcuaaa aaggacgccg aacuagagug cgaaaaacuc gggcugaaga     1680

uuaacucgaa aaauauacuu aaguugaggc uguucaaaga gcaaaaggaa uuuugugcau     1740

auucuggaga gaagaucaaa auuuccgauc uccaggacga gaagaugcug gaaaucgauc     1800

acaucuaccc cuauucacgg aguuucgacg auagcuacau gaacaaagug uuaguauuua     1860

ccaagcagaa ucaagagaaa cugaaccaga caccuuucga agcguuuggc aaugacuccg     1920

cuaaguggca gaagauugag gugcuugcca aaaacuugcc aacuaagaaa cagaagcgca     1980

uacucgauaa aaauuacaag gacaaggaac aaaagaacuu caaggauaga aaucugaacg     2040

acaccaggua uaucgcacgu cuaguccuga auuacacaaa agauuaucuc gacuuucugc     2100

cgcuuucuga ugacgagaac accaaguuga augacacgca gaaaggcagc aaggugcaug     2160

uugaggccaa gucagggaug cugaccagug cuuuacggca cacaugggga uucuccgcca     2220

aagaucgaaa caaccaccuc caucacgcaa uugacgcugu gaucauugcc uacgcgaaua     2280

acagcaucgu caaggccuuu ucugauuuca aaaaggaaca ggagucaaau agugcugaac     2340

uguaugccaa aaagauaagc gagcuugacu acaagaacaa aagaaaguuu uucgagcccu     2400

uuuccgguuu caggcagaaa gugcuggaua agaucgacga aauuuucgua ucuaaaccug     2460

agcgcaagaa accaucgggc gcauugcaug aagagaccuu ucggaaggaa gaggaguucu     2520

aucaaagcua cgggggaaag gaaggcguuc ucaaagcucu ggagcuaggu aagaucagaa     2580

aagugaaugg gaagauuguc aaaaacggcg auauguuuag gguggacauc uucaagcaca     2640

agaaaacuaa uaaguuuuac gccgucccca uauauacaau ggauuucgca cugaaagugc     2700

ucccuaacaa ggcgguugcc cgcuccaaaa agggagaaau uaaggacugg auccugaugg     2760

augagaauua cgaauuuugc uucucacuuu auaaagacag uuugauucug auccagacca     2820

aggauaugca ggagccagag uuuguguacu auaacgcuuu cacgucuagc accguauccu     2880

uaauagucuc aaaacaugac aauaaguuug aaacacucag caaaaaccag aagauccugu     2940

ucaagaaugc caacgagaaa gaagugauug caaagucuau cggcauucaa aaucuuaaag     3000

uuuucgagaa guacaucgug uccgcucugg gggaagucac uaaagccgag uuucgacagc     3060

gggaggacuu caagaaaaag cgucccgcag ccaccaagaa agcuggacag gcgaagaaaa     3120

agaaauaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa     3180

uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        3268


<210>  3567
<211>  3679
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3567
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaag cgaccugguu cucggacuug auauugguau aggcuccgug gggguaggaa      180

uccugaacaa ggugaccggc gagauuaucc auaaaaauuc uaggauuuuc ccagccgcgc      240

aggcugaaaa caauuugguc cggcgcacaa accgacaagg ucguagauua gccaggcgga      300

agaagcaccg cagagugagg cugaaucggc uauuugagga aucagggcuc aucacugacu      360

ucacgaaaau aaguaucaac cugaauccgu accagcuucg cguuaaggga cugaccgaug      420

aguugagcaa cgaagaacuc uuuauugcac ugaaaaauau ggugaagcau cgaggcaucu      480

ccuaucugga cgaugccucu gacgauggca acucgucagu cggggacuac gcucagauug      540

ugaaagagaa uagcaagcag cucgaaacaa agacucccgg acaaauccag uuagagagau      600

aucagaccua cggucagcug aggggcgauu ucacaguaga aaaagacggg aagaaacacc      660

ggcuuauaaa cguuuuuccu accagugcau aucguuccga ggccuugcgc auucugcaaa      720

cucagcagga guucaaucca cagaucacag augaauuuau uaacagauac cuagagaucc      780

ucaccggaaa gaggaaauau uaccauggcc ccgguaauga aaagucucgg acggacuacg      840

ggcgauaucg cacuagcgga gagacccugg auaacaucuu cggcauucuu auaggcaaau      900

gcacauuuua cccugacgag uucagagcug ccaaggcguc cuauacugca caagaguuua      960

accuguugaa ugaccucaac aaucugaccg ugccaacaga gaccaagaaa uuaucaaagg     1020

aacagaaaaa ccagaucauu aauuacguca agaacgagaa agccaugggg cccgcuaagc     1080

uguucaagua uaucgccaaa cuccuuaguu gugauguggc agacauaaag ggauacagga     1140

uugauaaaag cgguaaggcu gaaauccaca cguuugaggc cuaucggaaa augaagacuc     1200

uggaaacauu ggacaucgag caaauggauc gcgagacccu agacaagcug gcguacgugc     1260

ucacucugaa uacagaaaga gagggcauuc aggaagcccu ugagcacgag uucgcagaug     1320

ggucuuucuc ccagaaacag gucgacgagc ugguucaauu uaggaaggcu aacucaagca     1380

ucuucggaaa aggcuggcau aauuuuagug ugaaguugau gauggagcuc auuccugaac     1440

uguacgagac cucugaagaa cagaugacga uauuaacccg ucuggguaaa cagaagacaa     1500

cuuccagcuc gaacaagacc aaauauaucg augagaagcu acucacagaa gagauuuaca     1560

auccaguagu ggccaaaucu guccggcagg cuaucaagau cgugaacgcc gcaauuaaag     1620

aauaugggga cuucgauaau auaguuaucg agauggcccg agaaacuaac gaggacgacg     1680

agaagaaagc uauucaaaag auccagaagg cgaauaaaga ugaaaaggac gcagccaugc     1740

ugaaagcugc caaccaguac aacggcaagg cagagcuucc gcacuccgug uuucauggac     1800

acaaacaguu ggccaccaag auuagacugu ggcaccaaca gggcgaaagg ugccucuaua     1860

cagggaagac caucucaaua caugaucuga ucaauaacag uaaucaguuc gaggucgacc     1920

acauucuucc ccugagcauc acuuuugaug acuccuuggc uaacaaagug cucguauacg     1980

ccacggcaaa ucaggaaaag ggacaacgca caccuuauca ggcgcuggau ucuauggacg     2040

augcuuggag cuuccgggag uuaaaagccu uuguuagaga gucaaagacc cugaguaaca     2100

aaaagaagga auaccuacuc acugaggaag acauuuccaa auucgaugug aggaagaaau     2160

uuaucgagcg caaucugguc gacacccgau acgcaagccg ggugguccuu aacgccuugc     2220

aggaacauuu ccgugcucac aagauagaua caaaaguguc uguugugaga ggucaauuua     2280

ccucacagcu gaggcgccau uggggcauug agaagacucg ggacacauau caccaccaug     2340

ccguagacgc acucaucauu gcugccagua gccagcugaa uuuauggaag aaacagaaga     2400

acacgcuggu cuccuacucu gaggaucaac uuuuggacau cgaaaccggg gagcucauau     2460

cggaugacga auauaaagag agcguguuca aggcgccaua ccagcacuuc guugauacuc     2520

ugaaauccaa ggaguuugaa gacucaaucc uauucaguua ucagguggau ucuaaauuua     2580

auagaaagau uagcgacgcc acaaucuacg cuaccaggca ggcaaagguc ggaaaagaua     2640

aggccgacga gacauaugug cugggcaaaa uuaaggauau cuacacucaa gacgguuacg     2700

augcuuucau gaaaauauau aagaaggaca aauccaaguu ucucauguac cgacaugacc     2760

cccagaccuu cgaaaaagua auugagccua uccuggaaaa cuauccaaau aagcagauca     2820

acgagaaagg gaaggaggug cccuguaauc cguuucuuaa guacaaagag gaacacggcu     2880

auauucgcaa guacagcaaa aagggaaacg gcccugaaau caaaucauug aaguauuacg     2940

auucuaagcu ggggaaccac auugacauaa cgcccaaaga uuccaauaac aagguugucu     3000

uacagagugu gagcccaugg cgggccgacg uguacuucaa uaaaaccaca ggaaaguaug     3060

agauccucgg ucugaaauac gcagaucuuc aauuugaaaa gggcacuggg accuauaaga     3120

uuucucagga gaaauacaac gacaucaaga aaaaggaggg agucgauucc gacucagagu     3180

ucaaauuuac acuguauaag aaugauuugc uccugguuaa agacacugaa accaaggaac     3240

agcaacuauu cagauuucug aguaggacga ugccuaagca gaaacauuac guggagcuca     3300

agcccuauga uaaacagaag uucgaaggcg gugaggcgcu gauuaaagug cuugggaacg     3360

uagccaauag cggccagugc aagaagggau ugggcaaauc gaacaucucc auauacaagg     3420

uccguacaga cgugcugggg aaucaacaca ucauuaaaaa cgagggagac aagccaaaau     3480

uagauuucaa gcgccccgcu gccaccaaga aagcaggcca ggcuaagaaa aagaaauaag     3540

gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc     3600

uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaagaauu                                                  3679


<210>  3568
<211>  4237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3568
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaac ccaguucgag ggauuuacaa accuguacca aguuagcaag acucucaggu      180

ucgaacuuau uccacagggu aaaacgcuga agcauauaca ggagcagggc uuuaucgaag      240

aggacaaggc ccggaaugau cacuauaaag aauugaagcc gauuaucgac cgcauuuaca      300

aaaccuaugc ggaucaaugc uuacagcugg ugcagcuaga cugggagaac cucuccgcug      360

ccaucgauuc uuaccgaaag gagaaaacag aagagacucg uaaugcacug auagaggaac      420

aggccaccua uagaaacgcu auccaugacu acuucauugg gaggacagau aaucuuaccg      480

acgcaaucaa caagcggcac gccgagauuu auaagggacu guuuaaagcu gaauuguuca      540

auggcaaggu acucaaacaa cuggguacug ugacaaccac ggagcaugaa aacgcccugc      600

uccgcucauu ugauaaguuc acuaccuacu uuaguggguu cuacgagaau aggaaaaacg      660

ucuuuagcgc ggaagacauc uccacagcaa uaccccacag aauugugcag gauaauuucc      720

cuaaguuuaa agagaacugu cacaucuuca cucgguuaau uaccgccguu ccaucucugc      780

gcgagcauuu cgaaaaugug aagaaggcua ucggaaucuu ugucucgaca ucaauugagg      840

aaguguucag cuuucccuuc uauaaccagc uuuugaccca gacgcaaaua gaccuguaca      900

aucagcuacu cggcggcauc agucgagagg ccgggacuga gaaaauuaag ggacugaacg      960

aaguacuuaa ccuggcaauc cagaaaaaug augagacagc ucacauaauu gccuccuugc     1020

cucauagauu uaucccacuc uucaagcaaa uccugucuga caggaacacc cuuagcuuua     1080

uucuggaaga guucaaaucc gacgaagagg uuauccaguc auuuugcaag uauaagacuc     1140

uccuucggaa ugaaaacgug cuggagacag cggaggccuu guucaaugaa cuaaacagua     1200

uugaucugac ccacauauuu aucagccaca aaaagcucga gacgauuucu uccgcacugu     1260

gugaccauug ggauacccuu cguaaugcuc uguacgaacg cagaaucuca gaguugacag     1320

guaaaaucac uaagagcgcc aaagagaagg uccagaggag ucucaagcac gaagacauua     1380

accugcagga gauaaucucu gcugccggca aagaauuauc cgaggcauuc aagcagaaaa     1440

ccagcgaaau ucugucgcau gcccacgcug cgcuagauca accccucccu acaacucuga     1500

agaaacagga ggagaaggaa auccuuaagu cucaguugga cucccugcuc gggcuguauc     1560

aucuucugga uugguuugca guggacgagu caaaugaagu ggauccagag uucagugccc     1620

gguugaccgg aauuaaacuc gaaauggagc cgagccuguc cuucuacaac aaggcucgaa     1680

auuaugccac uaaaaagccc uacucugucg agaaauuuaa guuaaacuuc caaaugccua     1740

cccuggcaag cggcugggac guuaauaaag aaaagaacaa uggugccauc cuauuuguga     1800

agaacgggcu cuacuaucug ggaauaaugc caaaacagaa gggccgcuac aaagcucuuu     1860

cauucgagcc cacugaaaag acgagugagg gcuuugauaa aauguauuac gacuauuucc     1920

cugacgccgc aaagaugauc ccaaagugcu ccacacaguu gaaagcggua accgcucacu     1980

uucagacuca caccacaccc auucugcuca gcaacaauuu caucgaaccg cuggagauua     2040

ccaaggagau cuacgauuua aacaauccug aaaaagagcc caagaaauuu caaacugccu     2100

augcaaagaa gacaggggac cagaaaggau acagagaagc ccuguguaag uggauagauu     2160

ucacgaggga cuuucuuucu aaauacacca agacuacauc aauugauuug aguagccucc     2220

ggccauccuc ucaguauaaa gaccugggug aguacuaugc ugagcuaaac ccucugcucu     2280

accauaucuc guuccagcgc auugccgaaa aggagaucau ggaugcagug gaaaccggca     2340

agcuguaucu uuuucaaaua uacaauaaag acuucgcuaa ggggcaccau ggaaaaccca     2400

acuugcacac acuguauugg acuggcuuau ucagcccaga gaaucucgcc aagaccucca     2460

ucaaacugaa cggucaggcg gaauuauuuu acagacccaa gucaaggaug aaacguaugg     2520

cccaccggcu gggggagaag auguugaaua agaaacucaa ggaucagaaa acgccuauuc     2580

cagacacccu guaccaagag cuauaugauu acgucaacca ucgacugagu cacgaccucu     2640

cugaugaagc uagagcacug cuuccgaaug ugaucacaaa ggagguuagc caugaaauua     2700

ucaaagacag gcgcuucacu uccgacaagu uuuucuuuca cgugcccaua accuugaacu     2760

aucaggccgc uaauucaccu agcaaguuca accagcgggu caaugccuac cugaaagagc     2820

auccagaaac acccauuauc ggcaucgaua gaggagagag gaacuuaauu uauaucacug     2880

ugauugacuc uaccggcaag auacucgagc agcgcucccu gaacacgauc caacaguuug     2940

auuaccagaa aaagcuggac aaucgagaaa aagagcgggu agcagcgcgu caggccugga     3000

guguuguggg gacaauuaag gaucuuaagc aaggauaucu aagccagguc auccacgaaa     3060

uuguggaccu caugauccac uaccaggcug ucgugguucu ggagaacuug aauuucgguu     3120

uuaaaucuaa gagaaccggc auagccgaaa aagcagugua ccagcaauuc gagaagaugc     3180

ugaucgauaa auuaaacugc cucguacuga aggacuaucc ugcugagaag gucgggggag     3240

ugcuuaaucc auaccagcug acugaucagu uuacauccuu cgccaaaaug ggcacccaau     3300

cagguuucuu guuuuauguu cccgcaccgu acacaaguaa gauugacccu cucaccgggu     3360

ucguggaucc auuugucugg aaaacaauca agaaccauga aagcaggaaa cacuuccugg     3420

agggcuuuga cuuccuacau uaugauguga agacgggaga cuuuauucug cacuucaaga     3480

ugaaucgcaa ccuuucguuu cagcggggcc uccccggguu caugccugcc ugggacaucg     3540

uauuugagaa aaaugaaacc caguucgaug cuaagggaac ucccuuuaua gcggguaaaa     3600

gaauugugcc aguuaucgag aaccauaggu ucacaggccg auaccgcgac cuguauccug     3660

ccaaugaauu gaucgcacug uuagaggaaa aggggauugu cuuccgggau ggauccaaca     3720

uacuccccaa acugcuugag aaugacgauu cucacgcuau cgacaccaug guggcccuga     3780

uuagaagcgu guugcagaug aggaacucaa augccgcuac aggcgaagau uacaucaaca     3840

guccaguccg ugaccucaac gguguuuguu uugauucccg cuuccaaaau ccggaguggc     3900

cuauggacgc agaugccaac ggggcguacc acauugcucu gaagggccag cuacugcuua     3960

aucaucucaa agagucuaag gaccugaagu ugcagaacgg aaucagcaau caggacuggu     4020

uagccuauau acaagaacug cggaacaaaa gacccgcagc cacuaagaaa gcuggccagg     4080

caaagaaaaa gaaguaagga cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu     4140

gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                              4237


<210>  3569
<211>  4216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3569
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaag cauuuaccag gaguucguua acaaguauuc ccugucuaaa acccucaggu      180

uugaacuuau accacaagga aagacacugg agaauaucaa ggcccggggu uugauuuuag      240

acgaugaaaa acgcgcgaag gacuacaaaa aggcuaaaca gaucauugau aaguaucauc      300

aguucuuuau cgaggaaaua cugucaagug ugugcaucag cgaggaccua cuccagaacu      360

acuccgaugu auauuucaag cugaaaaagu cugacgauga caaucuucaa aaagauuuua      420

agucggccaa agacacuauu aagaaacaga ucucagagua cauuaaggau agcgaaaagu      480

ucaaaaaccu guuuaaucag aacuugaucg acgcaaagaa aggccaggag agugaucuca      540

uacuguggcu gaagcaaucc aaagacaaug ggauugaacu cuucaaggcc aacucugaca      600

ucacggauau ugacgaggcu uuagagauca ucaagagcuu uaaaggaugg accacauauu      660

ucaagggcuu ucacgaaaau cguaaaaacg uguacuccuc aaaugauauu ccgacaagua      720

uaaucuaccg aauugucgac gauaaccugc ccaaguuccu ugagaauaaa gcaaaguaug      780

agagcuugaa ggacaaagcc ccugaggcua ucaacuacga acagauaaag aaagaucugg      840

ccgaggagcu aaccuuugac auugauuaua agacaucuga agugaaucag agaguuuucu      900

cccucgacga gguguucgaa aucgcgaacu uuaacaauua ccugaaccag ucagguauca      960

ccaaauucaa uacuauuauc gggggaaagu uugucaacgg cgagaauaca aagaggaaag     1020

gcauuaacga auauauaaau cuuuacagcc aacagaucaa cgauaagacc cugaaaaagu     1080

auaaaaugag uguguuguuc aagcagauuc ucucugacac ggaguccaag agcuuuguaa     1140

ucgacaaacu ggaggaugac ucggauguug ugacuaccau gcaaucuuuc uacgaacaga     1200

ucgcagccuu uaagacaguc gaggaaaaau ccauuaagga gacuuuauca cuccuguucg     1260

acgaucuuaa agcucagaag cuggacuuga guaaaauaua cuuuaagaau gauaagagcc     1320

uaaccgaccu gucccagcaa guguucgaug acuauucugu gaucgggaca gccguccucg     1380

aauacauuac ccagcagauc gcacccaaaa accuggauaa uccaagcaag aaagagcagg     1440

agcuuauugc uaagaaaacg gaaaaggcca aguaucuguc auuggagacu aucaaacucg     1500

cgcuggaaga guuuaacaag caucgggaca uagauaaaca augucgcuuc gaggaaaucu     1560

uagccaauuu ugcagcuauu ccuaugaucu ucgacgagau ugcccagaac aaggacaauc     1620

uggcucagau caguauaaaa uaccagaacc aaggaaagaa ggaucuacuc caggccuccg     1680

cagaagacga uguuaaagcc auuaaggacc ugcuugauca gacaaacaau uugcugcaca     1740

aacucaagau cuuccauauu agccagucug aggacaaagc uaacauccug gauaaggacg     1800

aacacuuuua ucuuguguuc gaggagugcu acuuugaacu ggcgaauaua guaccauugu     1860

auaacaaaau cagaaauuac auuacccaaa agcccuacuc agaugagaag uucaaacuca     1920

acuuugaaaa uaguacucug gcaaacgguu gggacaagaa uaaagagccu gauaacacag     1980

ccaucuuauu cauuaaggac gauaaauauu accugggcgu gaugaauaag aagaacaaua     2040

aaaucuuuga cgacaaggcu auaaaagaaa acaaggggga gggauauaaa aagauugucu     2100

acaagcuacu cccaggcgcc aauaaaaugc ugccgaaggu guucuuuagc gcaaaaucca     2160

ucaaguucua uaaccccucu gaggauaucc uuaggauucg gaaccacucg acccauacga     2220

aaaaugguag cccucagaag ggguacgaaa aguuugaguu caacaucgaa gacugucgca     2280

aauuuauuga uuucuauaag caguccauau caaaacaccc agaguggaag gacuucggau     2340

uucgauucag ugauacccaa agauacaauu cuaucgacga auuuuacagg gagguugaga     2400

accagggcua uaaauugaca uucgaaaaua uuagcgaguc cuacaucgau ucagugguca     2460

accagggcaa gcuguaucuc uuucagauuu acaauaagga cuucagcgcc uauucuaaag     2520

ggcggcccaa ccugcauacu uuauacugga aggcucuguu ugaugaacgu aaucuucaag     2580

acgugguaua uaaauugaac ggagaggccg aacucuucua ccgcaagcag uccaucccua     2640

aaaagauaac ccacccagca aaagaagcga ucgcuaauaa gaacaaggau aaucccaaaa     2700

aggagagugu uuuugaauac gaccugauua aagacaagag auucacagag gauaaauuuu     2760

ucuuccacug cccgaucacu auuaacuuua agagcucugg ugccaauaag uucaacgacg     2820

aaaucaaccu acugcucaaa gagaaggcaa augaugugca uauacugucc auugacaggg     2880

gcgagcggca ccuugccuau uacaccuugg ucgaugggaa aggaaacauc aucaagcagg     2940

acacauuuaa uauuauaggc aacgaucgaa ugaaaaccaa uuaucaugac aagcuggcug     3000

ccaucgaaaa ggaucgcgac ucagcaagaa aagauuggaa gaaaauuaac aauaucaagg     3060

agaugaaaga agguuacuua agucaggugg uccacgagau ugcuaagcuc gugaucgaau     3120

auaacgccau aguuguguuc gaggaccuga auuuuggguu caagaggggc cgguuuaaag     3180

uagagaagca agucuaccag aaacuugaaa agaugcugau cgagaaauug aacuaucucg     3240

uguucaagga uaaugaauuu gacaaaacug gaggcguucu gcgcgcguac cagcuaacgg     3300

ccccuuucga gacauuuaag aagaugggga aacagaccgg aauuaucuac uaugugcccg     3360

cugguuucac uagcaagauu uguccaguca ccggcuuugu gaaccaacug uacccuaaau     3420

augaaucggu auccaagucu caggaguucu ucagcaaauu ugacaagauc ugcuacaauc     3480

ucgauaaggg guauuucgag uuuucauucg acuacaaaaa cuuuggagau aaggcagcca     3540

aaggcaagug gacaauagcu aguuucgguu ccagacugau uaauuuuagg aacucugaca     3600

aaaaucauaa cugggauacc cgugaagugu aucccacuaa ggagcuugaa aaguugcuga     3660

aagacuacag caucgaguac gggcacggcg aguguaucaa ggccgcaauu ugcggagaau     3720

ccgauaaaaa guucuuugcg aaauuaacau caguucucaa cacgauccug cagaugcgga     3780

auagcaagac cggcacugag cuugacuauc ugauaagucc agucgccgau gugaacggga     3840

auuucuuuga cucucgacaa gcucccaaga acaugccuca ggaugccgac gcaaauggag     3900

cuuaccacau uggucuaaaa ggccucaugc uguuggggag aaucaagaac aaucaggaag     3960

gcaaaaagcu gaacuuagug auuaaaaaug aggaauauuu cgaguuuguc cagaacagga     4020

auaacaagcg cccagccgca acuaaaaagg ccggacaagc uaagaaaaag aaauaaggac     4080

uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu     4140

cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa agaauu                                                     4216


<210>  3570
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3570
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaga caagaaauac      120

agcauuggac uggauauagg uacaaacucc guuggcuggg ccgugaucac agacgaguau      180

aagguaccau cuaagaaauu caaggugcuc gggaauacug auaggcauuc aauuaaaaag      240

aaccuuaucg gagcgcuguu guuugacagu ggcgaaacgg cugaggccac ccgguuaaaa      300

cgcacagcac gacguagaua cacuaggcgg aagaaucgca uuugcuaucu gcaggaaauc      360

uucagcaacg agauggccaa ggucgaugac uccuuuuucc acagacuaga agagucuuuu      420

cucguggagg aagauaaaaa gcaugagagg cacccgauau ucgguaauau cguugacgaa      480

guggcuuacc augagaaaua ucccaccauu uaccaucugc ggaagaaacu ugucgauucg      540

acagacaagg cagaucugcg cuugaucuau cucgcccugg cucacaugau uaaauuucga      600

gggcacuucc ugaucgaggg agaccucaac ccugauaauu cagacgugga caaguuauuu      660

auacaacugg uacagaccua caaccagcuu uucgaagaga auccaauuaa cgccagcggc      720

guugaugcga aggcaaucuu gagugccaga cuguccaaau cuaggcggcu agaaaaucuc      780

auugcucagc ugcccggcga gaagaaaaac gggcuuuuug gaaaucugau cgccuugagc      840

cucggucuga cuccuaacuu caaguccaau uuugacuuag cagaagaugc uaaacugcaa      900

cucucaaagg acacauacga ugacgaucuu gacaaccugu uggcccagau cggcgaucag      960

uaugcggacc uauuccuggc cgcaaagaac cucagugaug cuauucugcu uagcgacaua     1020

cugcguguga auaccgagau cacgaaagcc ccauugucug cuuccaugau uaagcgcuac     1080

gacgagcacc aucaggaucu cacucuguua aaagcccugg ucagacaaca gcuacccgaa     1140

aaguauaaag agaucuucuu ugaccaguca aagaacgggu acgcaggaua uauagauggc     1200

ggugccagcc aggaagaguu cuacaaguuu auuaaaccua uccucgaaaa gauggacggg     1260

accgaggagc ugcuugugaa auugaauagg gaagaucugc uccggaagca acgaacauuc     1320

gacaacggaa guaucccaca ccagauucac cugggcgagc uucaugcuau ccugcgcaga     1380

caggaagauu uuuauccguu cuugaaagac aauagggaga agauugaaaa gauacucacu     1440

uuucggaucc ccuacuacgu gggcccucug gcgcgcggga acucuagauu cgcauggaug     1500

accaggaaau ccgaggagac aauuacccca uggaauuuug aagaggucgu ugauaaggga     1560

gccagcgcuc aaucguucau cgaacguaug acgaacuuug acaaaaauuu acccaacgag     1620

aaggugcugc cuaaacacuc ucuacucuau gaguacuuca cuguauauaa ugaacugaca     1680

aaggugaaau acgucaccga ggguaugcgg aagccagccu uucuuuccgg cgaacagaag     1740

aaagcaaucg uggauuugcu guucaagacu aaccgaaaag uuacagugaa gcagcucaaa     1800

gaggacuauu ucaagaagau ugaauguuuu gauucagucg agauaagugg gguggaggac     1860

agauucaaug ccagccuggg aaccuaccau gacuuacuga aaaucauuaa ggauaaagac     1920

uuucuugaua acgaagagaa ugaagacauc uuggaggaua uuguacucac gcugacccua     1980

uucgaagaca gggagaugau cgaggaacgc cugaagacau augcucaccu cuuugaugac     2040

aaaguuauga agcagcugaa gcggagaagg uacacuggcu ggggucgccu uucccgaaaa     2100

uugauaaacg ggauccggga uaagcaaucu ggcaaaacca uucuggacuu cuuaaagagc     2160

gauggauuug ccaaccguaa uuucaugcag cucauccaug acgauucacu gacauuuaaa     2220

gaggacauuc agaaggcaca ggugaguggc caaggggacu cccuucacga acacaucgcg     2280

aaccuggcug gaagccccgc cauaaagaaa gguauuuugc agacugucaa gguggucgau     2340

gagcucguga aaguuauggg cagacauaag ccggaaaaua ucgugauuga gauggcaagg     2400

gagaaccaga ccacacagaa agggcaaaag aacucucgcg aacggaugaa aagaaucgag     2460

gaaggaauaa aggagcuggg cucacagauc cuaaaggagc acccuguaga aaauacccag     2520

cugcagaaug agaaacucua ccuguauuac cuucaaaacg guagggacau guaugucgau     2580

caggaauugg acauuaaucg acugagugau uacgacgugg aucauaucgu uccccagagc     2640

uucuuaaagg acgauuccau ugacaacaaa gugcucacuc gcucugauaa gaaucggggg     2700

aaaucggaca acgucccaag cgaggaagug guaaagaaga ugaaaaauua uuggagacaa     2760

cugcuuaacg ccaagcugau cacgcagagg aaauuugaca aucuaacaaa ggcugagcgu     2820

ggcggacucu ccgagcugga uaaagccggc uucauaaagc gccaguuggu ggaaacccgg     2880

cagauuacua agcacguugc acaaauccug gacucaagaa ugaacaccaa auacgaugag     2940

aacgacaagu uaaucaggga agucaaagug auuacacuca agaguaaacu ggugucugau     3000

uuucgaaagg acuuccaguu cuauaagguc cgggagauca auaacuacca ccaugcucac     3060

gaugccuacc uuaaugcggu uguggggacc gcccugauua aaaaguaucc uaaauuggaa     3120

agcgaguuug uguacggaga cuauaaggua uacgaugucc gcaaaaugau agcuaagucc     3180

gagcaggaaa ucgguaaggc aacugccaaa uauuucuuuu acucaaacau uaugaauuuc     3240

uuuaagacag agaucacgcu cgcuaacggc gaaauuagaa aaaggccccu gaucgagacc     3300

aauggggaaa cuggagagau agugugggac aagggccggg auuucgccac aguucgaaaa     3360

gugcuaagca ugccacaggu caacaucgug aagaaaaccg agguucaaac agguggguuu     3420

ucuaaggaau ccauucugcc caagagaaau agugacaaac uuaucgcacg caagaaagau     3480

ugggacccua agaaauaugg cggauucgac agcccaacug uggcguacuc uguacucguc     3540

guggccaagg uggagaaggg caaauccaag aaacugaagu cagucaaaga auugcugggg     3600

auuaccauca uggagaggag uagcuuugag aagaacccga uagauuucuu agaagcuaag     3660

ggauacaaag agguuaagaa agaccucauu aucaagcugc ccaaauauuc gcuuuuugaa     3720

cuggagaaug gccguaagcg gauguuggcc uccgcuggug aacuccagaa ggggaacgag     3780

cuggcacuac cuucuaaaua cgugaauuuc cuguaucuug ccagccauua cgagaagcuc     3840

aaaggaucac cagaagauaa cgagcagaag cagcuguucg uagaacaaca caaacauuau     3900

uuggacgaga ucauugaaca gauaagugag uuuuccaaga gagugaucuu agcagaugcc     3960

aaucuggaca aaguccucuc ugcuuacaac aagcacaggg auaagcccau ucgcgagcag     4020

gcggaaaaca ucauucaccu guucacgcuu accaaucugg gcgccccugc agcuuuuaaa     4080

uauuucgaca caacuaucga ucgaaagaga uacaccagca caaaagaggu guuggacgcc     4140

acucuaauac aucaauccau caccggucug uacgaaacgc ggauugaucu cucacagcug     4200

ggcggggaca agaggccagc cgcuaccaaa aaggcaggac aggccaagaa aaagaaauaa     4260

ggacuagugc aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau     4320

gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu     4380

cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa     4440

aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4530


<210>  3571
<211>  5313
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3571
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaaa cuucaagauu      120

cugccaauag ccaucgaccu cggaguuaaa aauaccggug uguuuagcgc guucuaccag      180

aagggcacau cccuugagag gcuggauaac aagaauggga aaguauauga auugucuaag      240

gacucauaca cuuuacugau gaacaaucgg acggcucgcc gacaucaacg uagaggaauu      300

gauaggaaac agcuagugaa gcggcucuuu aaacugaucu ggaccgagca gcuuaaccug      360

gaaugggaca aggauacaca gcaagccauu aguuucuugu uuaaucgcag aggcuucagc      420

uuuaucacug acgguuauuc cccggaguac cucaacauag uccccgaaca ggugaaggca      480

auccugaugg auauuuucga cgauuauaau ggggaggacg aucuggacuc uuaccucaaa      540

uuagccaccg agcaggaauc gaagaucuca gagauuuaua acaaacugau gcagaagauc      600

cuugaauuua aauugaugaa gcugugcaca gauauaaaag acgacaaggu uagcaccaag      660

acucuaaaag agauuacaag uuacgaguuc gaacuccugg cugauuaccu ugcaaauuau      720

uccgagucuc ugaagaccca aaaauuuagc uacacggaca agcagggaaa cuugaaagaa      780

cucuccuauu accaccauga uaaguauaau auccaggagu uccugaagag gcacgccacu      840

auuaacgacc ggaucuuaga uacccugcuc acagacgauc uugacaucug gaauuuuaac      900

uucgaaaaau ucgauuuuga caagaacgag gagaaacugc agaaucaaga agauaaggac      960

cauauucagg cucacuugca ccauuucgug uuugccguca acaaaauaaa gucagagaug     1020

gcgaguggcg gccgccaccg aagccaguac uuccaagaaa ucacuaaugu gcuagacgag     1080

aacaaucauc aggaagggua ucugaagaac uuuugugaga aucuccacaa caaaaaguac     1140

ucuaaucugu ccguuaaaaa ccuuguaaau cugauuggaa acuugucaaa ucucgagcug     1200

aagccuuuaa gaaaauacuu caacgauaag auccacgcaa aggccgacca uugggaugaa     1260

cagaaauuua ccgagacaua uugccacugg auacugggug aauggagggu gggcgucaag     1320

gaccaggaua aaaaggacgg ggcuaaauac agcuauaagg aucuauguaa ugagcucaaa     1380

caaaagguga ccaaggccgg acugguggac uuccuuuugg aacuggaucc augccggacg     1440

auucccccuu accucgacaa caacaaucgu aaaccaccca agugucagag ucugauccuu     1500

aacccuaaau uucuggauaa ucaguaucca aacuggcagc aauacuugca ggagcucaag     1560

aaacugcagu cuauccagaa uuauuuagac uccuucgaga cugaucugaa gguccuaaag     1620

agcucgaaag accaaccgua cuuuguugaa uacaagucuu ccaaccagca gauugcauca     1680

ggccagcgcg acuauaaaga ucucgacgcu agaauccugc aauucauuuu ugauagggug     1740

aaggccagug acgagcuuuu gcugaaugaa auauacuucc aggcgaaaaa gcucaagcag     1800

aaagccagcu ccgagcugga gaaguuagaa ucuagcaaaa agcuggauga gguaaucgca     1860

aacucacaac uuagucagau uuugaaaucc cagcauacaa augguaucuu cgaacagggg     1920

accuuucucc accuggugug caaguauuac aagcaacggc agcgagcucg cgacagcaga     1980

cuauauauca ugcccgagua cagguaugau aaaaagcugc auaaauacaa caauacugga     2040

cgguucgacg augacaacca gcuccugaca uacuguaauc acaagccucg ccagaaaaga     2100

uaucaacuuu ugaacgaucu ggccggcguc uuacaggugu cuccaaauuu ucucaaggac     2160

aaaauuggcu cagaugacga ccuguucaua aguaaguggc uuguugaaca caucaggggg     2220

uuuaagaaag caugcgagga uagccugaag auucagaaag acaaccgugg auugcucaac     2280

cauaagauca auauugcccg gaacaccaaa gguaagugug agaaggaaau cuucaaucug     2340

auaugcaaaa ucgagggcuc cgaagauaag aaagggaacu acaagcacgg acuagcauau     2400

gagcugggcg ugcuccuguu uggugaaccc aaugaggccu cuaaaccuga guucgaccga     2460

aagauuaaga aauuuaacuc gaucuacagc uucgcucaga uucaacagau cgcguuugca     2520

gaaagaaagg ggaaugccaa cacgugugcu gucugcuccg ccgauaaugc acauaggaug     2580

cagcaaauaa aaauuaccga gccaguggaa gacaacaagg auaaaaucau ucuuucagcc     2640

aaggcucagc gcuugcccgc caucccgaca cggauaguag acggcgcagu uaagaaaaug     2700

gcgacuaucc uggcuaagaa uauuguggau gacaacuggc agaauaucaa acaggucuua     2760

agugccaagc accaacucca cauuccuauc auaaccgagu cuaacgcauu cgaauuugag     2820

cccgcccugg cugaugugaa aggaaagagc cuuaaggaca gaaggaaaaa ggcccuggag     2880

cgcauuuccc cagaaaauau cuucaaagac aagaacaacc gaaucaaaga guucgcaaag     2940

ggcauuucag cuuauagcgg ggccaaucua acagauggag acuuugaugg ugcgaaagaa     3000

gaacucgacc auaucauucc ucggucucac aagaaguacg gcacucugaa cgaugaggcc     3060

aauuugauau gcgucacccg uggggacaac aaaaauaagg gaaacagaau cuucugucug     3120

agggauuuag cugacaauua uaaacucaag caguuugaaa caaccgauga ccuggagauu     3180

gagaaaaaga ucgcagauac uauuugggac gccaacaaga aagauuucaa guuuggcaau     3240

uaccgcuccu ucaucaaccu uacgccccag gagcagaagg cuuuucggca ugcccuguuc     3300

uuggcagacg aaaauccaau aaaacaagcg gugaucagag ccauuaacaa uaggaaccga     3360

acauuuguua augguaccca gcgcuacuuc gcugaggugc ucgccaacaa uaucuaucug     3420

cgggcuaaaa aggagaaccu aaacacugac aagauuaguu uugauuacuu cgggaucccc     3480

accauaggca auggaagagg cauugcugaa aucaggcagc uguaugagaa aguagacagc     3540

gauauccagg ccuacgcaaa gggggacaaa ccucaagccu cuuauuccca ccuuauugau     3600

gcuaugcucg cguucugcau agccgcagac gaacaucgua acgauggauc aaucggucug     3660

gagauugaca agaauuacag uuuguaucca cuggauaaaa acacaggcga agucuuuacc     3720

aaggacaucu ucagccagau uaagaucacu gauaaugagu uuucggacaa aaaguuagug     3780

cgcaaaaagg cuauagaggg guucaacaca caccggcaga ugacgagaga uggaaucuac     3840

gccgaaaauu accucccgau ucugauccac aaagagcuua acgaaguuag gaagggcuau     3900

accuggaaaa auuccgagga aauuaagauc uuuaagggua aaaaguacga cauacagcaa     3960

cugaacaauu ugguguauug ucucaaauuc gucgacaagc ccauuucuau cgauauccag     4020

auuagcacuc uggaggagcu acgaaacauc cugacaacca auaacauagc cgcuacagca     4080

gaauacuauu acauuaaucu uaaaacucag aagcuccaug aguauuacau cgaaaacuac     4140

aacaccgccc ugggguauaa gaaauacuca aaggagaugg aguuuuugcg gaguuuagcg     4200

uaucgcuccg aaagagugaa aauuaagucu aucgacgaug uaaaacaagu gcuggacaag     4260

gauagcaauu ucauaauugg caagaucacg cucccuuuua aaaaggagug gcagaggcug     4320

uaccgggaau ggcagaacac cacaaucaaa gacgauuaug aguuccuuaa guccuuuuuc     4380

aauguuaaau caauuacuaa gcugcacaag aaaguccgaa aggacuuuag cuugccaauc     4440

aguaccaacg aaggaaaauu ccuagugaag agaaaaacau gggauaauaa cuucauuuac     4500

cagauacuga augacucuga uucccgcgcu gacggcacua agcccuuuau cccugccuuc     4560

gacauuagca aaaacgagau cguggaggca aucauugauu cguuuaccuc aaagaauaua     4620

uucuggcucc caaagaacau cgaacugcaa aaagucgaca auaagaacau uuuugccauc     4680

gauacgucua aaugguucga gguugaaaca cccagugacc uuagggauau ugggaucgcu     4740

accauacagu auaagaucga caauaacucc cguccgaaag ugcgggugaa guuagauuac     4800

guaauugacg augacagcaa gaucaauuac uuuaugaacc auucucugcu caaaagcaga     4860

uauccugaua aggucuugga gauucugaaa caguccacua ucauagaguu cgaaucaagu     4920

ggauuuaaca agacaauuaa agagaugcuc ggcaugaagc uggcagguau cuacaaugaa     4980

accagcaaca auaagaggcc agccgcgacu aaaaaggcug ggcaggccaa aaagaaaaag     5040

uaaggacuag ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa     5100

aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc     5160

ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau     5220

aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5280

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  5313


<210>  3572
<211>  3585
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3572
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaaa gaggaacuac      120

auucugggac ucgacauagg uaucacauca guuggcuaug ggauuaucga uuacgagaca      180

cgggacguga uugaugccgg aguacgccuu uucaaagaag cgaaugugga gaacaaugaa      240

ggccgacguu ccaagagagg ugcuaggcgg cugaagcgca gaaggcggca ucgcauccag      300

cgagucaaaa aguuguuauu ugacuauaac cugcuaacug aucacucuga gcucucaggg      360

auaaauccau acgaagccag agugaaagga cugagucaaa aacuuagcga ggaggaguuc      420

uccgcagccc uguugcaucu cgcuaaaagg cggggcguuc acaacgugaa ugaagucgaa      480

gaggacacgg gcaacgagcu gucuaccaag gaacagaucu cgcguaauuc aaaggcacug      540

gaggaaaaau auguggccga gcuccaguua gaacgccuga agaaagaugg ggagguaaga      600

ggaagcauua acagguuuaa gacaagugac uacguuaaag aggcuaagca gcuuuugaaa      660

gugcaaaagg ccuaucauca gcuggaucag uccuucaucg acacuuacau ugaucuacuc      720

gaaacccggc gaacauacua ugaggguccg ggcgaagggu cucccuuugg auggaaggac      780

aucaaagagu gguacgaaau gcugaugggc cacugcaccu auuucccuga ggagcuucgc      840

agcgucaagu acgcguauaa ugcagaucug uacaacgccu ugaaugaccu caacaaucug      900

gugauaacua gagacgaaaa cgagaaauua gaauauuacg agaaguuuca gauuaucgaa      960

aacguguuca aacaaaagaa gaaaccaaca cugaagcaga uugcuaaaga gauccucguc     1020

aaugaggaag auaucaaggg uuacaggguu accuccacgg ggaaacccga guuuacuaac     1080

cuuaaggugu aucacgacau uaaggauaua accgcccgga aagaaaucau ugagaaugca     1140

gagcuguugg accagaucgc uaagauacua acaauuuacc agucaaguga agauauccaa     1200

gaggaacuga cuaaccucaa uagcgagcug acccaggaag agaucgagca gauuucuaac     1260

cuuaaaggau auacaggcac ccauaaucug uccuugaagg ccaucaaccu cauucuggac     1320

gaauuauggc acacgaauga uaaccaaaua gcgaucuuca aucgccugaa acuuguaccu     1380

aagaaggugg accucucaca gcagaaagag auuccaacua cacuggucga ugacuuuauc     1440

cuuagccccg ugguuaagag aaguuucauc cagucuauua aagugauaaa cgccaucauu     1500

aagaaauacg gcuugccuaa ugauaucauu aucgaacugg caagggagaa gaacuccaaa     1560

gacgcucaaa agaugauaaa ugaaaugcag aagcguaacc ggcagaccaa cgagcgaauc     1620

gaggaaauua ucagaacuac agggaaagag aaugccaagu aucucauuga aaagaucaag     1680

cugcaugaca ugcaggaggg aaaaugucuu uacagccugg aagcuauacc auuggaggau     1740

cuccugaaca auccguuuaa cuaugagguc gaccacauua uccccagguc ggugucuuuc     1800

gauaauuccu ucaacaauaa gguauuaguu aagcaagaag agaacucaaa gaaggguaau     1860

cgcaccccuu uucaguaccu gaguagcucc gacucuaaaa uuagcuacga aacguucaag     1920

aaacacaucc uaaaccucgc caagggcaag gggcggauau caaaaaccaa gaaagaguau     1980

cugcuugagg aaagagauau caauagguuu agugugcaga aggacuucau uaaccgcaau     2040

uuggucgaua cacgauacgc aacucgggga cugaugaacc uccugcguuc cuauuuuaga     2100

gugaauaacu uagacgucaa agugaaaagc aucaacggcg guuucaccuc uuuucugagg     2160

cgcaagugga aauucaagaa agagcggaau aagggguaca aacaucacgc cgaagaugcu     2220

cuuauuaucg cgaacgcaga cuuuauauuc aaggagugga aaaaguugga uaaggccaaa     2280

aagguuaugg aaaaucagau guuugaggag aaacaagcug aaucaaugcc agagauugaa     2340

acagagcagg aauauaagga gaucuucauc acuccccauc agauuaaaca caucaaggac     2400

uuuaaggauu acaaauauag ucauagagug gacaagaaac cuaacaggga gcucauuaau     2460

gacacccugu acagcacacg aaaggaugac aaaggcaaca cccuaauagu aaauaaccug     2520

aauggacucu acgauaagga caacgauaag cugaaaaagc uaaucaauaa auccccagaa     2580

aaguugcuga uguaucacca cgacccccag acuuaccaaa aauuaaagcu cauuauggag     2640

caguauggcg augaaaagaa cccgcuguac aaauauuacg aggaaacggg gaauuaucuu     2700

acaaaguacu cuaaaaagga caacggaccu gucaucaaaa agauuaagua cuaugguaau     2760

aaacugaacg cccauuugga uaucaccgac gauuacccca acucgcgcaa uaaggugguu     2820

aaacucagcc ugaagccaua ucgguucgac guguaccuag auaacggcgu cuauaaauuc     2880

gugacuguaa agaaucugga cgugaucaaa aaggagaacu acuaugaggu uaauuccaag     2940

ugcuacgaag aggccaaaaa gcucaaaaag auaucaaacc aggcagaauu uauugcuagu     3000

uucuacaaua acgaccugau caaaauuaau ggggagcuuu auagagucau cggagugaac     3060

aaugauuugc ugaacaggau agaggugaau augauugaca ucaccuaccg ugaauauuua     3120

gagaacauga augauaagcg cccuccccgg aucauuaaga caauagccuc uaaaacccaa     3180

agcaucaaga aauacuccac ugacauucuc ggcaaccugu augaagucaa gagcaaaaag     3240

cacccacaga ucauuaagaa agguaagaga cccgcagcga ccaaaaaggc ugggcaggcc     3300

aaaaagaaga aauaaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa     3360

uaagagaaag aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua     3420

aagccaacac ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu     3480

gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3585


<210>  3573
<211>  4653
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3573
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcacu guucaacaag      120

ugcauuauaa ucagcauuaa ucucgacuuu uccaacaaag agaaguguau gaccaagcca      180

uacucuaucg gacuugauau ugguacaaau ucaguuggcu gggccgugau cacugacaac      240

uauaaaguac cgaguaagaa aaugaaggug cuggggaaua cgagcaaaaa guacauaaag      300

aaaaacuugu uaggaguccu gcuauucgau uccggcauca ccgcggaagg uaggcggcuc      360

aagcgcacag cucgacguag auauacuagg cggcgcaaua ggauucugua ccuucaggag      420

aucuuuucua ccgaaauggc cacacuggac gaugcauucu uucaaagauu ggacgauucg      480

uuccucgugc ccgacgauaa acgggacuca aaguauccua uuuuugggaa ccugguugag      540

gaaaaagugu accaugauga guucccaacc aucuaucauc ugcgcaagua ccucgccgac      600

agcacuaaaa aggcugauuu acgacugguc uaccuugcau uggcccacau gauaaaguau      660

agaggacacu uucugauuga gggcgaguuc aauaguaaaa acaaugacau ccagaagaac      720

uuucaggacu uccuagauac auacaaugcu auuuuugagu ccgaccucuc ucuggaaaac      780

agcaaacagc uugaggagau cgugaaggau aaaaucucca agcuggaaaa gaaagacagg      840

auuuugaaac ucuuccccgg cgagaaaaau ucagggauau ucagugaauu ucugaaguua      900

aucguaggaa accaagccga uuuccggaaa ugcuuuaacc uggacgagaa ggcgagccuc      960

cauuucucua aggaauccua ugaugaggac cuugagaccc uguuggguua cauuggcgau     1020

gacuauucag auguuuuucu aaaagcaaag aaacuguacg acgccauccu ccugagcggg     1080

uuccuuacgg ugacugacaa ugaaaccgag gcuccucuga guucugccau gauaaagcgu     1140

uauaacgaac acaaagagga uuuggcacuc cugaaggaau acauucgcaa uaucuccuua     1200

aagacauaca acgaggucuu uaaagacgau acuaagaaug gauaugcugg cuacaucgac     1260

gguaaaacca accaggagga uuucuaugug uaccugaaga aucuacucgc cgaauuugag     1320

ggggcggacu auuuccugga aaagauugau agagaggacu uucuuaggaa gcagcggaca     1380

uucgauaacg gaagcauccc auaccagauu cacuugcaag aaaugcgagc cauacuggac     1440

aaacaggcaa aguuuuaucc cuuccucgcu aagaauaaag agcgcaucga gaagauucug     1500

accuucagaa ucccuuacua cgugggccca cuugccaggg gcaacucgga uuuugcuugg     1560

ucuauccgga aacgcaauga aaagauuacg ccguggaacu ucgaggacgu cauagauaaa     1620

gaauccucag ccgaggcauu uaucaauaga augacaaguu ucgaccugua uuugcccgag     1680

gaaaagguuc ucccuaagca uagccuguua uacgagacuu uuaacgugua uaaugaacug     1740

accaaaguaa gguucauugc cgaguccaug cgugacuacc aguuucuaga uucuaagcag     1800

aaaaaggaca ucgugcggcu cuauuucaaa gauaagcgaa aggucacuga caaagauauu     1860

aucgaauacc ugcacgcuau auauggguac gacggaaucg agcuuaaggg uauugagaaa     1920

caauuuaaca gcucauugag uacauaccau gaucugcuca acaucauuaa ugacaaggag     1980

uuccuggaug acuccagcaa cgaagcgauc auagaagaga uuauccacac cuuaacgauu     2040

uuugaagaua gagagaugau caaacagagg cugucuaagu ucgagaauau auuugacaag     2100

ucagugcuua aaaaguugag ucgccggcac uauaccggcu gggggaaacu cagcgcaaag     2160

cugaucaacg gaauuagaga ugaaaaaucc ggcaauacaa uccuagacua ccugauugac     2220

gaugguaucu cuaacaggaa uuucaugcag cucauacaug acgaugcccu gucguucaag     2280

aaaaagauuc aaaaggcuca gaucaucggg gacgaggaua aaggcaacau uaaggaaguu     2340

gugaaaagcc uuccaggauc ccccgccauc aagaaaggca uuuugcaguc aauaaagauc     2400

gucgacgagc uggugaaggu aaugggaggg cgcaaaccug aaaguauugu uguggagaug     2460

gcacgagaga aucaguauac uaaccaaggu aagucuaaua gccagcagcg guuaaaacgu     2520

cucgaaaagu cccugaaaga gcuuggcuca aagauccuga aggaaaacau uccagccaaa     2580

uugagcaaga ucgauaauaa cgcucuccag aaugacagac uguaccuaua uuaccugcaa     2640

aacgggaaag auauguauac cggagacgau cucgacauag acaggcuguc uaacuacgau     2700

aucgaccaca uuauccccca ggccuuucuu aaggauaauu ccauugacaa caaagucuug     2760

gugaguagcg caucuaaucg cggcaagucc gaugacuucc cgucacugga ggucgugaag     2820

aaacggaaga cauuuuggua ccaguuacuc aaaaguaagc ugaucagcca gagaaaauuc     2880

gauaaccuua cuaaggcgga gagggguggg cugcuaccug aagacaaggc uggcuuuaua     2940

caacgacagc ucguugagac ccgccagauu acaaaacaug uggcccggcu guuggaugaa     3000

aaguucaaua acaaaaagga cgagaauaac agagcaguaa ggaccgucaa aaucaucacu     3060

cugaagucga cguuaguguc ccaguuucgu aaagauuucg aacucuauaa gguucgcgag     3120

auuaaugacu uucaccaugc ccacgaugcu uaccugaacg ccgugauagc aucugcccuu     3180

cugaagaaau aucccaaguu ggagccagag uucgucuacg gagacuaucc uaaauacaau     3240

agcuuucggg aaagaaaguc agcuacagaa aaaguguauu ucuacaguaa caucaugaau     3300

auuuucaaga aguccaucuc ucucgcggac ggcaggguaa uugagcgacc ccugaucgaa     3360

gugaacgagg agaccgggga aagcguuugg aauaaagagu ccgaucuagc cacuguccgg     3420

cgcgugcugu cauacccaca agugaacguc guuaagaaag uggaagaaca gaaccacgga     3480

cuugacagag guaagcccaa aggccucuuu aaugcuaacc ugagcaguaa gccuaagcca     3540

aauucuaacg aaaauuuggu gggggcaaaa gaguaucugg auccgaagaa auacggaggc     3600

uaugccggua uauccaacag cuucgcugua uuagucaagg ggaccaucga gaaaggcgcc     3660

aagaagaaaa uuacaaaugu gcucgaauuu cagggaaucu cgauucugga caggaucaac     3720

uaccggaagg auaaacuuaa uuuccuguug gagaagggcu auaaagacau agaacucauu     3780

aucgagcugc ccaaguacuc acuauuugag cugucugaug ggagucgaag aaugcuugca     3840

uccauccuca gcaccaacaa uaaacgcgga gaaauucaua agggcaacca aaucuuccug     3900

ucucagaagu uuguuaaauu guuauaucac gcgaagagga uaagcaauac uauuaacgag     3960

aaucaucgua aauacgugga aaaccacaag aaagaguucg aggaacuguu uuacuauauc     4020

cucgaguuca augaaaacua cgucggugcc aagaagaacg ggaaacugcu uaauuccgcu     4080

uuucagucau ggcagaacca caguauugac gagcugugua gcucuuucau cggaccuaca     4140

ggcuccgaac ggaaggguuu guuugagcua acgucaagag gcagugccgc agauuucgag     4200

uuccuggggg ugaaaauacc aagguaucgc gacuacaccc ccagcucccu ccugaaggau     4260

gcuacucuua uucaucaauc uguuacagga uuauaugaaa cccgaaucga ccuggccaaa     4320

cucggcgagg ggaagagacc ugcagccaca aagaaagcug gacaggcgaa gaaaaagaaa     4380

uaaggacuag ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa     4440

aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc     4500

ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau     4560

aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  4653


<210>  3574
<211>  3729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3574
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaug guacgccagc      120

cugauguccg cgcaucaccu caggguugga auugacgugg guacccauuc uguaggccuu      180

gcuacacugc ggguggauga ccacgggacu ccaauagagu uguuaucagc ccugagucau      240

auccacgaua gcggagucgg caaggaaggu aaaaaggacc acgauacgcg caagaaacua      300

uccgggauug cacgacgugc cagaaggcuc cugcaucacc ggcgcaccca gcuucaacag      360

cuggacgagg uguugagaga ucucggauuc ccgaucccca caccuggcga auuucuggac      420

cugaacgagc agacugaucc auauaggguu uggcgggugc gcgcucgacu cgucgaagag      480

aaguuacccg aggaacugag aggcccugca auuucuaugg ccgugaggca uaucgcucgg      540

caccgugggu ggcgcaaucc auacucgaaa guagagucac uuuugagccc cgccgaagag      600

aguccuuuca ugaaggcgcu gagagagagg auacuagcaa ccacaggaga aguucucgac      660

gaugguauca ccccaggcca ggccauggcu caaguggccc ugacucacaa cauuuccaug      720

cgggggccgg agggaauccu uggcaaacug caucagucug acaaugcaaa cgaaauucga      780

aagaucugcg cucgccaggg ugucagcccc gaugugugua agcaguugcu cagagccgug      840

uuuaaagcgg acuccccuag ggggucagcc gucagucggg uugcaccaga cccccugccu      900

ggacaaggca gcuuccgcag agcuccaaag ugcgaucccg aguuucagag guuccguaua      960

auuucuaucg uggccaauuu acggauuucc gaaacaaaag gcgagaaccg accgcugacc     1020

gcugacgaga gaaggcacgu agugacguuu cucacugaag auucacaggc cgaccuuacc     1080

ugggucgaug uggcagagaa gcuggggguu caucgccggg acuugagagg aacagccgug     1140

cacacugaug acggugaaag gagcgcugcg cgcccuccca ucgaugcaac cgaccgaauc     1200

augcggcaga caaaaauuag uucucuaaag accugguggg aggaagccga uuccgagcaa     1260

cguggcgcua ugauaagaua ucuguacgag gacccaacgg acagcgaaug ugccgagauc     1320

auugcagaac ucccugagga agaucaggcc aaacuggacu cgcuucaucu gcccgcuggg     1380

agggccgcau auucucgcga guccuugacu gcgcucucag aucacaugcu ggcuacaacc     1440

gacgauuuac acgaggcccg gaagagacug uucggagucg acgauaguug ggcaccaccc     1500

gccgaagcua ucaaugcccc ugugggcaac ccaagcguag acaggacucu aaagauaguu     1560

ggucgauacc ucuccgcagu ggagucuaug ugggggacac cggaagucau ucauguggag     1620

cacguccgcg auggcuuuac cagcgagcgg auggcugacg aaagagauaa agccaauagg     1680

cgucgcuaua acgacaauca ggaggcgaug aagaaaaucc aacgggauua cggaaaggaa     1740

ggcuacaucu caagagggga cauugugagg cuggacgccc uugaguugca gggaugcgcu     1800

ugucuguauu gcgguacgac caucggcuac cauacauguc agcucgauca cauuguuccc     1860

caggcagggc cuggaaguaa caaucgacgg ggcaaccugg uggccguaug cgaacgcugu     1920

aauagaucca aaagcaacac uccauucgcu gucugggccc aaaagugcgg uauaccccac     1980

guggggguua aggaggcaau cggcagggug cggggauggc gaaaacagac cccuaacaca     2040

ucuucagagg accuuacuag acugaagaaa gaagucauug cgcgcuugag gcguacccag     2100

gaggauccag aaaucgacga gcggaguaug gaaagcgugg ccuggauggc uaaugagcuc     2160

caucacagaa ucgccgcagc uuaucccgag acaaccguaa ugguguacag gggcuccauu     2220

acugccgcag cccgcaaggc ugcggggaua gauucucgaa ucaaccugau uggagaaaaa     2280

gguagaaagg accggaucga uaggcgccau cacgccguug acgcaucggu cguggcuuua     2340

auggaggcca gcguggccaa gacgcuggcu gaacguuccu cacuaagagg cgagcagcgg     2400

cucacaggga aagaacaaac cuggaagcag uauacuggaa guaccgucgg cgcaagggag     2460

cauuuugaga uguggcgcgg ucacaugcug caccuuacag aauuguucaa ugagcgacug     2520

gccgaagaua aaguuuacgu gacccagaac auuagacucc ggcugucuga cgggaaugcg     2580

cauacuguga acccgagcaa guuaguaucc cacaggcugg gcgauggacu uacaguccag     2640

caaaucgacc gcgcuuguac gccugccuug uggugcgcac ucaccagaga gaaagauuuu     2700

gacgagaaga auggccugcc agccagggaa gaccgggcua uacgugugca ugggcacgag     2760

aucaagucaa gcgauuauau ucagguuuuc ucuaaacgca agaaaacuga cuccgaucga     2820

gacgaaacac ccuucggagc aaucgccgug agaggcgguu uugucgagau ugggccuagu     2880

auccaucacg cgaggauaua ccggguggaa ggaaagaaac ccguuuacgc uaugcuacgc     2940

guguucaccc acgaucugcu cagccagaga cauggcgacc uguuuucugc cguaauucca     3000

ccucagucca ucucaaugag gugcgcagag cccaaacuuc ggaaagccau uacaacuggu     3060

aacgcuaccu auuugggcug ggucguggug ggggaugagc uggaaaucaa ugucgacagu     3120

uucacgaagu acgccauagg acgauuuuua gaggauuucc caaacaccac aagauggcgc     3180

aucuguggcu augacacuaa uagcaagcuc acccugaaac cgauuguucu ugcagcugaa     3240

gggcuggaga acccuucguc cgccgugaau gaaaucguag aguugaaggg auggagggug     3300

gcgauuaacg uccucacaaa agugcacccc acuguugugc ggcgugaugc ccugggcaga     3360

ccaagguacu cuagccgcuc aaaucuaccc accaguugga cgaucgagaa gcggccugca     3420

gcuacaaaga aggccgguca agcuaagaaa aagaaauaag gacuagugca ucacauuuaa     3480

aagcaucuca gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau     3540

cucuuuuucu uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu     3600

aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaagaauu                                                             3729


<210>  3575
<211>  4590
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3575
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaac caagccauac      120

agcauuggac uggacauagg uacaaacucc guuggcuggg ccgugacuac ggauaauuau      180

aaaguaccgu cuaagaagau gaaagugcuc gggaacaccu caaagaaaua caucaagaaa      240

aaucuucugg gagucuuguu auucgacagu ggcauuacag cggaggguag gcggcugaag      300

cgcacugcuc gacguagaua uaccaggcgg cgcaacagaa uccuauaccu ccaggaaauu      360

uuuagcacag agauggccac ccuggaugac gcauucuuuc aaaggcuuga ugacuccuuc      420

cuggugcccg augacaagcg ggauucuaaa uauccuaucu uugggaauuu gguugaagag      480

aaggccuacc augacgaguu cccaacuaua uaucaccucc gcaaauaccu ggcugauucg      540

acaaagaaag cagaccugcg acucguguac uuagcccugg cucauaugau caaguauaga      600

ggacacuuuc uuauugaggg cgaguucaac ucaaagaaua acgauaucca gaagaauuuu      660

caggacuucu uggacaccua caacgccauu uuugaaagcg aucugagucu agaaaauucc      720

aagcagcucg aagagaucgu caaagacaag auaucuaaac uggagaagaa agauaggauu      780

cuuaagcugu uccccggcga aaagaacagc gggaucuuuu ccgaguucuu gaaacucauu      840

gugggaaauc aagcggacuu ccggaagugc uuuaaccugg augaaaaagc aucauuacau      900

uucaguaagg agagcuauga cgaagaucug gagacgcucc uugguuacau cggcgacgau      960

uauucugacg uauuucugaa agccaagaag uuguacgaug cuauccuacu guccggguuc     1020

cucacuguua ccgacaauga gacagaagcc ccucugucaa gcgcaaugau uaaacguuau     1080

aacgagcaca aggaagaccu ugcucuguug aaagaguaca uacgcaacau cagucucaag     1140

acuuacaaug aaguguuuaa agaugacacc aagaacggau augccggcua cauugauggu     1200

aagacaaauc aggaggacuu cuaugucuac cugaaaaagu uacuggcgga guuugaaggg     1260

gccgauuauu uccuagagaa aaucgacaga gaagauuuuc ucaggaagca gcggaccuuc     1320

gacaacggau cuauaccaua ccagauucac cuucaagaga ugcgagcaau ccuugauaaa     1380

caggcuaagu uuuaucccuu cuuggccaaa aauaaggaac gcaucgagaa gauucugacg     1440

uuuagaaucc cuuacuacgu gggcccacuc gcuaggggca acuccgacuu cgccuggagc     1500

auucggaaac gcaaugagaa gauaacuccg uggaacuucg aagaugugau cgacaaagag     1560

ucgucugcag aagccuuuau uaauagaaug acauccuucg aucuguaucu ucccgaggag     1620

aagguccugc cuaaacauuc auugcucuac gaaaccuuua acguuuauaa ugagcugacu     1680

aaggugaggu ucaucgcuga aaguaugcgu gacuaccagu uuuuagacag caagcagaaa     1740

aaggauaucg uacggcugua uuucaaagac aagcgaaaag ugacagauaa ggacauuaua     1800

gaguaccuac acgcgaucua uggguacgau ggaauugaac ucaaggguau cgagaaacaa     1860

uuuaacuccu cucugagcac cuaccaugac cuuuugaaua uuaucaacga uaaggaguuc     1920

cuggacgauu caaguaauga agcaauaauc gaggaaauua uccacacgcu caccauuuuu     1980

gaggacagag aaaugaucaa acagaggcug uccaaguucg agaacauauu ugauaaaagc     2040

gucuuaaaga agcugucucg ccggcacuau acaggcuggg ggaaacuuuc agccaaguug     2100

auuaacggaa ucagagacga gaaaaguggc aauacuauuc ucgauuaccu gaucgacgac     2160

gguauaagca acaggaauuu caugcagcua auccaugaug acgcucuguc cuuuaagaaa     2220

aagauucaaa aagcccagau cauuggggau gaagacaagg gcaacaucaa ggaggugguu     2280

aaaucucucc caggaucgcc cgcaauaaag aaaggcauuc ugcagagcau caagaucgug     2340

gaugaacuug ucaaagugau gggagggcgc aagccugagu ccauuguagu ugaaauggcc     2400

cgagagaauc aguauaccaa ccaagguaag ucaaauaguc agcagcgguu gaaacgucug     2460

gagaagucuu uaaaagaacu cggcagcaag auccugaaag agaacauucc agcuaagcug     2520

uccaagauag acaauaacgc ccugcagaau gauagauugu accucuauua ccugcaaaac     2580

gggaaagaca uguauacagg agaugaccua gauaucgaca ggcugucaaa uuacgacauu     2640

gaucacauca uuccccaggc auuccucaag gacaacagca ucgauaauaa agugcugguc     2700

ucuuccgcga guaaccgcgg caagagcgac gaugugccgu cucuugaagu cgugaaaaag     2760

cggaagacuu ucugguacca guugcugaaa uccaaguuaa uaucacagag aaaauuugac     2820

aaccucacca aggcugagag gggugggcug aguccugagg auaaagccgg cuucauccaa     2880

cgacagcuug uugaaacacg ccagauuacc aagcaugugg cacggcugcu agacgagaag     2940

uuuaauaaca aaaaggauga aaauaacaga gccguaagga cugucaaaau cauuacgcuc     3000

aagagcacac uggugucgca guuccguaaa gacuuugagu uguauaaggu ucgcgaaauc     3060

aaugauuucc accaugcuca cgacgccuac cugaacgcag uggucgcuuc cgccuuacuc     3120

aaaaaguauc ccaagcugga gccagaguuu guguacggag auuauccuaa auacaauucu     3180

uuccgggaaa gaaagagcgc gaccgagaaa guauauuuuu acucaaacau aaugaauauu     3240

uucaagaaaa guaucucccu ugccgacggc agggugaucg aacgaccccu gauugagguu     3300

aacgaagaga cuggggaguc ugucuggaau aaggaaagcg acuuggcuac cgugcggcgc     3360

gugcucuccu acccacaagu caacguugug aagaaagugg aggaacagaa ucacggacug     3420

gauagaggua agcccaaagg ccuauuuaac gcaaaucugu caagcaagcc uaaaccaaac     3480

aguaacgaga aucuuguagg ggccaaggaa uaucucgacc cgaagaaaua cggaggcuau     3540

gcugguauau cuaacuccuu cacaguccug gugaagggga ccaucgagaa aggcgccaag     3600

aaaaagauua cuaauguuuu ggaguuccag ggaaucagca uucuggauag gaucaacuac     3660

cggaaggaca aauuaaauuu ucuccuggaa aagggcuaua aagauauaga gcuuaucauu     3720

gaacugccca aguacucguu guucgagcuc ucagacgggu cucgaagaau gcuggcaagu     3780

auccuaucca caaacaauaa acgcggagag auucauaagg gcaaccaaau cuuucugagc     3840

cagaaauucg ugaaacuucu cuaucacgcg aagaggauau cuaauacgau uaacgaaaau     3900

caucguaaau acgucgagaa ccacaagaaa gaauuugagg aacuguucua cuauaucuug     3960

gaguuuaaug agaacuacgu gggugccaag aaaaauggga aguuacugaa cagcgcuuuc     4020

caguccuggc agaaucacuc aaucgaugaa cucuguagua gcuuuauugg accuaccggc     4080

ucugagcgga agggucuguu cgaacuuacu uccagaggcu cagccgcaga cuuugaguuc     4140

cuggggguua aaaucccaag guaucgcgau uacacaccca guagcuugcu aaaggacgcu     4200

acccugauac aucaauccgu gacaggacuc uaugaaacuc gaauugaucu ggccaaacuu     4260

ggcgagggga agagaccugc agccaccaaa aaggcuggac aggcgaagaa aaagaaauaa     4320

ggacuagugc aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau     4380

gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu     4440

cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa     4500

aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4590


<210>  3576
<211>  3672
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3576
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcagc cgcguucaag      120

ccaaacagca uuaauuacau acugggacuc gacaucggua uugcuuccgu uggcugggcc      180

augguggaga ucgaugaaga ggaaaacccg auuaggcuua ucgaccuggg gguacgggug      240

uuugagcgcg cagaaguccc caaaaccgga gauucuuugg ccauggcucg acguuuagca      300

agaucaguga ggcggcugac acgcagaagg gcccaucggc uacuccgcac ucgaagacug      360

cuuaagaggg agggcguucu gcaggcugcc aauuucgacg agaacgguuu gauaaagagu      420

cucccuaaua cgccauggca acugcgggcg gcagcccugg aucguaaacu cacccccuua      480

gaauggagcg cugugcugcu ucacuugauc aagcaucgcg gguaucuguc ccagaggaaa      540

aacgagggag aaacagccga caaggagcua ggcgcacucc ugaaaggcgu cgcugggaau      600

gcccacgcgc uucagacugg agauuuuagg accccugccg agcuggcauu gaacaaguuc      660

gaaaaggagu cuggucauau ucggaaucag cgaucggacu acucacacac auuuagccgc      720

aaagaucucc aagcugaacu gaucuuacug uucgagaagc agaaagaauu uggcaaccca      780

cacgugagug ggggacucaa ggagggcauu gagacccuuc ugaugacuca gagacccgcc      840

uuguccggug acgcuguaca gaaaaugcua gggcauugca cauucgaacc ugccgagcca      900

aaggcagcca aaaauaccua uacggcugaa agguuuaucu ggcugacuaa gcucaacaau      960

cugcggauac uugagcaagg aucugaacgc ccgcugaccg auacagagag agcgacuuug     1020

auggacgagc ccuacagaaa gagcaaacuc accuaugcac aggcccguaa gcuguuaggc     1080

cuggaagaua cagcuuucuu uaaaggccua cgguacggga aggacaacgc cgaggcaucc     1140

acccucaugg aaaugaaagc cuaucacgcu auuucacgag cccuggagaa ggaaggacuu     1200

aaggacaaaa agaguccuuu gaaucugagc ccagagcucc aggaugagau cgguacggca     1260

uucucucugu uuaaaacuga cgaagauauu acaggcagac uuaaggacag gauccagccc     1320

gagauccugg aagcguugcu caaacauauu uccuucgaua aguucguuca aauaucacug     1380

aaggcuuuac gccggaucgu gccucugaug gagcagggga aaagauacga cgaggccugu     1440

gcagaaauuu acggagauca cuauggcaag aaaaacaccg aggaaaagau cuaccuacca     1500

cccauaccgg ccgacgagau uaggaacccu gucgugcucc gcgcucugag ccaggcccga     1560

aaagugauca auggugucgu ucggcguuau gggagucccg caagaaucca cauugaaacu     1620

gcuagggagg ugggcaaguc uuuuaaggau cgcaaagaga ucgaaaagcg gcaagaggaa     1680

aacagaaaag acagggagaa ggccgcggcc aaauuccgag aauacuuucc aaauuucgua     1740

ggagagccua aguccaaaga uauucuuaag uugcgccugu augagcagca gcauggcaag     1800

ugccucuaca gcgggaaaga aauaaaccug ggacgguuaa augagaaggg uuauguggaa     1860

aucgaccacg cucugcccuu uucgagaaca ugggacgauu cuuucaacaa uaaaguccuu     1920

guguugggcu ccgagaacca gaauaagggg aaccaaaccc cauacgaaua cuuuaaugga     1980

aaagacaacu caagggagug gcaggaguuc aaggcacgug uugaaacgag ucgcuuuccc     2040

cggagcaaga aacagaggau ucuccugcag aaguucgaug aggacggcuu uaaagaaaga     2100

aaucuaaacg auacccgaua ugugaaucgg uuccuguguc aauuugucgc cgaccgcaug     2160

agacucacag guaaggggaa aaagagggug uucgcuucca acggccagau cacuaaccug     2220

cuucggggau ucuggggcuu gcgaaaggua agagccgaga augaucgcca ucacgcacug     2280

gacgcgguug uggucgccug cucuaccgug gcuaugcagc agaaaaucac aagguuuguc     2340

cguuacaagg agaugaacgc cuucgauggg aaaacuauug acaaggaaac cggagaggug     2400

uuacaucaga aaacacacuu uccucagcca ugggaguucu uugcacagga aguuaugaua     2460

cggguguucg guaagccgga uggcaagccc gaauuugagg aggcugacac ccucgaaaaa     2520

cugagaacuc uucuggccga gaaguugagc ucaaggccug aagcaguaca cgaguauguc     2580

acgccacucu ucgugagucg cgcccccaau cgaaaaaugu ccgggcaggg acauauggaa     2640

acaguuaaga gcgcuaaaag acuggaugag ggcgugucug uccuacgggu gccucugacc     2700

caacucaagc ugaaagaccu ugagaagaug guaaacaggg aacgcgagcc aaaguuguac     2760

gaagcgcuga aagcccguuu agaggcacac aaggacgauc ccgcuaaagc cuuugccgaa     2820

ccguucuaua aguacgacaa agcugguaau agaacucaac aggugaaggc aguucggguc     2880

gagcaagugc agaagaccgg gguguggguc aggaaccaua auggcaucgc cgauaacgcg     2940

acaaugguuc gcguggacgu guuugagaaa ggagauaagu auuaccucgu accuauuuac     3000

ucauggcagg ucgcuaaagg cauccugcca gaccgagccg ugguucaggg gaaggaugaa     3060

gaggacuggc aacuuauuga ugacaguuuc aauuuuaaau ucagccugca ccccaacgau     3120

cuaguggaag ucaucaccaa gaaggcaaga auguucggau auuuugccuc cugucaccgg     3180

ggcacuggua auauaaacau caggauucau gaccucgauc acaaaaucgg gaagaauggc     3240

auucuggagg gaaucggugu gaaaacagcu uugucuuucc agaaguacca gauagacgag     3300

cugggcaaag aaauucgccc uugcagauua aagaagaggc ccccaguucg gaaacguccu     3360

gcagccacga agaaagcggg gcaggcuaag aaaaagaagu aaggacuagu gcaucacauu     3420

uaaaagcauc ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu     3480

caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc     3540

uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga     3600

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaagaa uu                                                         3672


<210>  3577
<211>  4428
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3577
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaaa gaaaccauac      120

accauuggac uggacauagg uacaaacagc guuggcuggg ccgugcucac ugaucaguau      180

gaccuuguaa agaggaagau gaaaaucgcg ggggauuccg agaagaaaca aauuaagaaa      240

aauuucuggg gagugcggcu guuugacgaa ggccagacgg cugccgaucg ccgaauggca      300

cguaccgcca gaaggcggau cgagcgcaga aggaaccgga uuucuuacuu gcaggguauc      360

uucgcugaag agaugucaaa gacagacgca aacuuuuucu gccgcuuaag ugauagcuuu      420

uaugucgaca augaaaagcg aaauuccaga cauccguucu uugccacuau agaggaggaa      480

guggaguacc acaaaaacua ucccaccauc uaccaucuga gggaggagcu aguuaauucu      540

ucggaaaagg cugaucuccg gcugguguau cuugcccugg cgcacauuau caaauaccgu      600

gggaacuucu ugauugaagg agcacucgac acacagaaua ccucagucga uggcaucuac      660

aagcaauuua uacagacuua uaaccaggug uucgccagcg gcauugagga cgggagucug      720

aaaaagcugg aagauaauaa agacguagcu aagauccucg uugagaaggu gacacgcaaa      780

gaaaaguuag agaggauucu gaaacuuuac ccuggagaga aguccgccgg uauguuugca      840

caguucaucu cuuugaucgu cggcagcaaa gggaacuuuc aaaagccauu cgaccugauu      900

gaaaaguccg auauagagug ugcuaaagac ucauaugaag aggaucuaga aagucuccug      960

gcccuuaucg gagacgagua cgcggagcug uucguggccg caaagaaugc uuauagcgcc     1020

guggucuugu cuuccauuau caccguugcu gaaacggaga cuaacgccaa acucucagca     1080

agcaugauag aaagguuuga uacccaugag gaagaccugg gcgaguuaaa ggccuucauu     1140

aaacugcacc uccccaagca cuacgaggaa aucuuuagua auacagagaa gcaugguuau     1200

gcuggguaca ucgauggaaa aacuaagcag gcggacuucu acaaauauau gaagaugacc     1260

cuugaaaaca uugagggcgc agauuacuuu aucgccaaaa uugagaagga aaacuuccug     1320

cggaagcagc gaacauuuga caauggcgcu auaccucacc aguugcaucu agaggaacug     1380

gaggccaucc uccaccaaca ggcaaaauau uacccauucc ugaaggaaaa cuaugauaaa     1440

auuaagucuc uugugaccuu ucgcaucccc uacuucguag ggccucuggc caauggacag     1500

uccgaguuug cuugguugac gagaaaagcc gacggugaga ucaggccaug gaacauugaa     1560

gagaaggugg acuucggcaa aagcgcaguc gauuuuauag aaaagaugac uaauaaggac     1620

acauaucucc cgaaagagaa cgugcugccc aagcacucgu uaugcuacca aaaauaccug     1680

guuuauaaug aacuaaccaa ggugcgguac aucaacgauc aggggaaaac uucuuauuuc     1740

uccggacagg agaaggagca gauuuucaau gaccucuuua agcaaaaacg caaggucaaa     1800

aagaaagauc uggaacuuuu cuugagaaac augucacaug uggagagucc uacaaucgaa     1860

ggccuggagg acagcuuuaa uuccucuuac agcaccuauc acgaucuccu gaagguaggu     1920

auuaagcagg aaauccuuga caacccaguu aauacggaga ugcuggagaa cauagugaaa     1980

aucuugaccg ucuucgaaga uaagaggaug auuaaagagc agcuccagca auuuucagac     2040

gugcuggaug gggucguguu aaagaaacug gaacgucggc auuacacagg cuggggacga     2100

cuaagugcga agcuccugau gggcaucaga gacaagcagu cccaccuuac uauuuuggau     2160

uaucugauga augacgacgg gcucaacagg aaccugaugc aguuaaucaa ugauagcaac     2220

cugucuuuca aaucaauaau ugagaaggag cagguuacca cagcugacaa agauauccaa     2280

aguauugugg ccgaccuugc aggaagcccc gccaucaaga aagguauauu gcagucccuc     2340

aagaucguag augaacuggu cucugugaug ggcuacccuc cacagacuau uguuguggag     2400

auggcucgcg aaaaucagac cacagggaaa ggaaagaaca auucgcggcc cagauacaag     2460

agccuagaga aagccaucaa ggaauuuggc ucccaaauuc ugaaagagca uccgaccgac     2520

aaccaggagc ucaggaauaa ccgccuguau cuuuacuauu ugcagaaugg uaaggauaug     2580

uacacugggc aagaccugga uauccacaac uuaucaaauu augacauaga ucacauuguc     2640

ccucagaguu ucaucacgga caacucuauc gauaaucucg ugcugacaag cuccgcaggc     2700

aaccgagaaa aaggagacga cguaccccca cuugagauug ugcggaagcg uaaggucuuu     2760

ugggaaaaac uguaccaggg caauuugaug ucaaagagaa aauucgauua ucucaccaag     2820

gcugagagag ggggacugac ugaagccgac aaagcgcgcu uuauccaucg gcagcuaguu     2880

gagaccagac aaauuacaaa gaacguggcc aacauacugc accagagguu caauuacgag     2940

aaggaugacc augguaacac caugaaacag gugcgaaucg ucacucucaa gagcgcucug     3000

guuucucagu uucgcaaaca auuccagcuu uacaaggugc gggaugugaa ugacuaucac     3060

cacgcacaug augccuacuu gaacggcgua gucgcuaaua cacuguuaaa aguguauccu     3120

cagcucgaac ccgaguucgu uuacggggac uaucaccagu uugauugguu caaggccaac     3180

aaggcaacgg cgaaaaagca auuuuacacc aauauuaugc uguucuuugc ccagaaagac     3240

agaaucauug augaaaacgg agagauccuu ugggacaaga aauaucugga cacugugaag     3300

aaggucaugu ccuacaggca gaugaauaua gugaaaaaga cagaaaucca aaaaggcgag     3360

uucaguaagg cuaccauuaa accaaagggu aacagcucua aacuaauccc ccguaagaca     3420

aauugggauc cuaugaagua cgggggccuc gacuccccaa acauggccua ugcaguugug     3480

auugaguacg cuaaaggaaa gaauaaacug guauuugaaa agaaaaucau acgcgucacu     3540

auuauggagc ggaaggccuu cgaaaaggau gagaaagcau uuuuggagga acagggcuau     3600

agacagccga aggugcuggc caaauuaccc aaguacaccc ucuaugagug ugaagagggg     3660

aggcgacgga ugcuggcuuc agcgaacgaa gcccagaaag gaaaucaaca ggugcuuccu     3720

aaccaucugg ucacguugcu ccaccaugca gcuaacugcg agguuaguga cggcaagagc     3780

cuggauuaca ucgagucgaa ucgcgaaaug uucgccgagc uacuggccca cguguccgaa     3840

uuugcuaaga gauauacccu ugcagaggcc aaccucaaua aaaucaacca gcuguucgaa     3900

cagaauaagg agggugacau uaaagcgaua gcucaaucuu ucguagauuu gauggccuuu     3960

aacgcaaugg gggccccagc uagcuucaag uuuuucgaga caacuaucga aaggaaacgg     4020

uacaauaacc ugaaggaguu acucaauuca accauuaucu accagaguau uacaggacug     4080

uaugaauccc gaaagagacu ugacgauaaa cgccccgcag ccacuaagaa agcgggccag     4140

gcuaagaaaa agaaauaagg acuagugcau cacauuuaaa agcaucucag ccuaccauga     4200

gaauaagaga aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu     4260

guaaagccaa cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc     4320

ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  4428


<210>  3578
<211>  4461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3578
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaaa gaaaccauac      120

agcauuggac uggacauagg uaccaacucc guuggcuggg ccgugguaac agaugacuau      180

aaggugccgg cgaagaaaau gaagguccuc gggaauacug auaaaucuca uaucgagaag      240

aaccuucugg gagcuuuguu auucgacuca ggcaauacgg ccgaagauag gcggcugaaa      300

cgcaccgcac gacguagaua cacaaggcgg cgcaaccgga uucuauaucu ccaggagauc      360

uuuagugaag agauggguaa gguggacgau agcuucuuuc acaggcugga agacuccuuc      420

cuuguuacug aggauaagag aggggagcgc caucccauuu uuggaaaucu ggaagaggaa      480

gugaaauacc acgagaacuu cccuaccauc uaucauuugc gacaauaccu cgccgacaau      540

ccagagaagg ucgaucugag acugguguau cucgcuuuag cacacauaau caaauuuagg      600

ggccacuucc ugauugaagg caaguuugac acacggaaca augauguaca gcgucuuuuc      660

caggaguuuu uggccguuua cgacaacacc uucgaaaauu cuucgcugca ggagcaaaac      720

gugcaggucg aagagauccu aacugacaaa auuucaaaga gcgcuaaaaa ggaucgcgug      780

cucaagcugu uucccaauga gaaaaguaac gggagauucg ccgaguuccu uaagcugauc      840

gugggaaauc aggcggacuu uaaaaagcau uucgaguugg aagagaaagc accucuccag      900

uuuuccaagg auacauacga agaggagcug gaagucuuac uggcccaaau aggugacaac      960

uaugcugaac ucuuccuuuc ugccaagaaa cuguacgaua gcauuuugcu auccggcauc     1020

cugaccguua cggacguggg gacuaaggca ccacucucag cuaguaugau ucagagguau     1080

aacgaacacc agauggaucu ggcccagcuu aaacaauuua uccggcagaa gcugagcgac     1140

aaauacaaug agguauucuc ugaugugucc aaggacggau augcgggcua caucgauggu     1200

aagaccaacc aggaagccuu uuauaaauac uugaaggggc uccugaauaa aauugaggga     1260

ucaggcuacu ucuuagacaa gauagagcga gaagacuuuc ugcgcaaaca aagaacauuc     1320

gauaacggca gcauccccca ucagauucac cuacaggaga ugagggcaau cauacggcgc     1380

caggcugaau uuuauccuuu ccucgccgac aaucaagaua gaauugagaa gcugcuuacu     1440

uuuaggaucc cauacuaugu cgggccguug gcucguggaa agagugacuu cgccuggcug     1500

ucucggaaau ccgcagauaa gaucaccccc uggaacuucg acgagauugu ggauaaagaa     1560

agcucggccg aggcuuuuau caaucgaaug acaaacuacg accucuaucu gccuaaucag     1620

aagguucuuc caaaacacuc ucuguuguac gaaaaguuca ccguguauaa cgagcucacg     1680

aaagucaagu acaagacuga acaggguaaa acagcguuuu ucgaugcaaa uaugaagcag     1740

gagauuuuug acggcguguu caaaguauac agaaagguua ccaaagauaa gcugauggac     1800

uuuuuagaga aggaguucga ugaauuuagg auaguggacc ugacugggcu agacaaagaa     1860

aacaaggucu ucaaugccuc cuauggaaca uaccaugauc ucugcaaaau ccuggacaag     1920

gauuuucuug acaacaguaa aaaugagaag auuuuggaag auaucgugcu gacccucacg     1980

cuguucgagg accgcgagau gauccggaag agauuagaaa acuauucaga ucugcuuacc     2040

aaagagcaag ucaagaaauu ggaaaggcgc cacuacacag gcuggggucg acucagcgcu     2100

gagcugauuc augggauacg gaacaaggaa ucccguaaaa cuauccuaga cuaucugauu     2160

gaugacggca auucuaacag aaauuuuaug cagcucauca acgaugacgc ccugagcuuc     2220

aaggaggaga uugcaaaggc ccaggugauc ggagaaaccg auaaucuuaa ccagguugug     2280

ucagacauag cuggcagucc cgccaucaaa aaggggauuu ugcaaucccu gaaaaucgua     2340

gacgaguuag ucaagauuau gggacaccag ccugaaaaua ucgugguuga gauggcaagg     2400

gagaaccagu ucacaaauca gggucgccgg aacagccaac agagacucaa aggccugacu     2460

gauucuauaa aggaauuugg gucacagauu cuuaaagagc auccagugga aaauagucaa     2520

cugcagaacg acagguuguu ccucuacuau cugcagaaug gacgagauau guacaccggc     2580

gaggaacuag acaucgauua ccugagccag uaugacauug aucacaucau accccaagcg     2640

uuuaucaagg acaacuccau ugauaaucgc guccucacau cuucgaagga gaaccggggu     2700

aaaagcgacg augugccguc caaggacgua gugagaaaaa ugaagucaua cuggaguaaa     2760

cugcuuucug cuaaguugau cacccagagg aaguucgaca accugacuaa agccgagcgu     2820

gggggcuuaa cggaugacga uaaggcagga uuuauuaaac gccagcucgu ugaaacacgg     2880

cagaucacca agcacgucgc cagaauacug gacgagaggu ucaauacuga aaccgaugag     2940

aacaauaaaa agauucgaca agugaagauc gugacacuua aaagcaaccu ggucuccaau     3000

uuucggaagg aguucgaacu auauaaaguu cgcgagauca acgacuacca ucacgcucau     3060

gaugccuauc ucaaugcagu gauuggcaag gcucuguugg ggguguaccc ucagcuggaa     3120

cccgaguuug uauauggaga cuacccacac uuccacgguc auaaagaaaa caaggccacc     3180

gcgaagaaau uuuucuacuc aaauaucaug aacuucuuua agaaagauga cgucagaacu     3240

gauaagaaug gcgagauuau auggaaaaag gacgaacaca ucagcaacau uaagaaagug     3300

uuaucuuauc cucagguuaa uaucgugaag aaagucgagg agcagacagg gggauucucc     3360

aaggaaagua uucuccccaa aggcaacagc gauaagcuga ucccaaggaa aacgaagaag     3420

uuuuacuggg acaccaaaaa guaugguggg uucgacucuc ccauaguggc cuacuccauc     3480

cuuguuauug cugauaucga gaaaggcaag aguaaaaagc ugaagacugu gaaagcauug     3540

guaggaguca caauuaugga aaagaugacc uuugagcggg acccuguggc cuuccucgag     3600

cgaaaaggcu auagaaaugu gcaagaagag aacaucauaa agcugccaaa auacucacua     3660

uuuaagcugg aaaacgggcg caagaggcuu cucgcuagcg cccgugagcu gcagaaagga     3720

aaugaaauug ucuugccgaa ccaucugggc acauuacucu aucacgcaaa gaauauccau     3780

aaaguugaug agcccaagca ccuggacuac guggauaaac acaaggacga guucaaggaa     3840

cuucuggaug uagugucgaa cuuuuccaaa aaguacacuu uggcggaggg uaaucucgag     3900

aaaaucaagg aacuguaugc ccagaacaau ggggaagacc uaaaagagcu ggcuucuagc     3960

uucauuaacc uucucaccuu uacggccaua ggagcuccug caaccuucaa guuuuucgau     4020

aaaaauaucg accggaagag auacacauca acuaccgaga uucugaacgc cacauugauc     4080

caucaaagua uuacuggcuu auaugaaacc aggaucgauc ugaauaagcu cgguggcgac     4140

aaacgcccag cagccacgaa gaaagcuggg caggcgaaga aaaagaagua aggacuagug     4200

caucacauuu aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa     4260

uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac     4320

auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa     4380

gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaagaau u                                               4461


<210>  3579
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3579
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaca gacaacaaac      120

cugagcuaca uucucggacu ugaccugggu auagccuccg uuggcugggc ggugguagag      180

aucaaugaaa acgaggaucc aauuggguug aucgacgugg gagucaggau uuucgaacgg      240

gcugaggugc cgaagacugg cgaaucuuua gcccugucac gccgacuagc acguaguacg      300

agaaggcuca uccggcgcag agcccauagg cugcuucugg cuaaacgguu uuugaagcgc      360

gaggguauac ucagcaccau cgaucuggag aaggggcugc ccaaucaagc augggaacuc      420

cgaguugccg gauuagagag aaggcugucc gcuauugaau ggggcgccgu gcuuuugcac      480

cugaucaaac aucggggcua ucuaucuaag cguaaaaacg agucgcagac aaauaacaag      540

gagcucgggg cgcugcuuuc aggagucgca cagaaucacc agcuguugca aagcgacgau      600

uaccgcacuc cugccgaacu cgcucugaaa aaguucgcca aggaggaagg ucauauuaga      660

aaccagaggg gcgcauauac ccacacauuu aaucgguuag accugcucgc ugagcuuaac      720

cuguuguucg cccagcagca ccaauuuggg aauccacauu gcaaagaaca cauccagcag      780

uacaugaccg agcuacugau guggcagaag cccgcgcuca guggagaggc cauacugaaa      840

augcuuggca aguguacuca ugagaaaaac gaguucaagg cagcuaaaca cacauauucc      900

gccgaacgau uuguguggcu gaccaaguug aauaaccucc gcauucugga ggauggugcu      960

gaaagagccu uaaaugagga ggaaaggcaa cugcuaauca accacccuua cgagaagucu     1020

aaacucacgu augcacaggu acggaagcug cuuggguuga gcgaacaggc cauuuucaaa     1080

caucugcgcu acuccaagga gaacgcugaa ucagcgacuu uuauggagcu caaagcaugg     1140

cacgccauca gaaaggcucu ggagaaucaa ggacuuaagg acaccuggca ggaucuggcc     1200

aaaaagccag acuugcucga ugaaaucggc acagcauuca gucuguacaa aacugacgag     1260

gauauucagc aguauuuaac caacaagguu cccaauagcg ugauaaacgc ccugcuaguc     1320

ucucucaauu uugacaaauu caucgaacug ucccuuaagu cauugaggaa gauucugccu     1380

cucauggagc aaggcaaacg uuacgaccag gcuugccggg agaucuaugg gcaucacuac     1440

ggagaagcca accagaagac aagccagcug uuaccagcaa uaccggcgca agagauucga     1500

aaucccgugg ugcugagaac ccuuagucag gcuaggaaag ucaucaacgc caucauucgc     1560

caguaugguu cuccugcacg gguucauauc gaaacgggca gagaguuggg gaaguccuuu     1620

aaagaaaggc gcgagauuca gaagcaacag gaggauaauc gaacuaagcg ggaaagcgcc     1680

gugcagaaau ucaaggaacu cuucucggac uuuucuuccg aaccaaaauc aaaggauaua     1740

cugaaauucc gucuauacga gcagcaacac ggaaaguguc uguauagugg caaagaaauc     1800

aacauucaca gacucaauga gaaggguuac guagagaucg accaugcucu gcccuuuagc     1860

aggacauggg augacuccuu caacaauaag gugcuugucu uggccucuga aaaccagaau     1920

aaagggaacc agaccccuua cgaguggcug caaggcaaga ucaacagcga acgcuggaaa     1980

aauuuugugg cauuaguucu cggaucacag ugcagugcug ccaagaaaca gcggcugcuu     2040

acucagguga uugaugacaa caaguucaua gauagaaauc ugaacgacac aagguauauc     2100

gcgcgauuuu uguccaauua cauucaagag aaccuccugc uagucggcaa gaauaaaaag     2160

aacguguuca ccccaaaugg gcagaucacg gcccugcucc gcagccggug gggacugauu     2220

aaagcuagag aaaacaauaa caggcaccau gcacuugaug ccaucguagu ugcuugugcc     2280

acccccucua ugcagcagaa gauaacacgu uuuauccgcu ucaaagaggu gcacccguau     2340

aagauugaga aucgguacga aauggucgac caagagucag gugaaaucau uaguccucac     2400

uuucccgagc caugggcaua uuucagacag gaggugaaca ucagggucuu ugacaaucau     2460

ccugauacug uguugaagga aaugcugccc gaccgaccac aggcgaacca ccaguucguu     2520

caacccuuau ucgugagccg ggccccuacc cgcaaaaugu ccggccaggg gcauauggag     2580

acaauaaagu cugcuaaaag acucgccgaa ggaauuucgg uacugaggau cccacuuacu     2640

cagcugaagc cgaaccuacu cgagaauaug gucaacaaag aacgggagcc cgcacuguac     2700

gcuggcuuga aggcccgacu ggcagaguuu aaucaagauc cugccaaggc uuucgcgacc     2760

ccauuuuaua aacagggugg gcaacaggug aaggccauua gaguugaaca ggugcagaaa     2820

agcggcgucu uagugcgcga gaacaaugga guagcagaca acgcuuccau cgugaggaca     2880

gauguuuuca uaaagaauaa caaauuuuuc cucgucccca ucuacaccug gcaaguggcc     2940

aagggcauuc ugccuaauaa agccaucgug gcucacaaga acgaagacga gugggaagag     3000

auggaugagg gggcaaaguu uaaauucuca cuuuuuccaa augaccuggu cgaauugaag     3060

acuaaaaagg aguacuucuu uggauauuac auuggucucg aucgugccac gggcaacauc     3120

agucugaaag aacaugacgg ggagauaucu aagggaaagg auggcguuua ucgggugggu     3180

gugaaacuag cgcugagcuu cgaaaaguac cagguagacg agcuugggaa aaauagacag     3240

auuugcaggc cccagcagcg ccagccgguc cgaaagagac cugcugccac caaaaaggca     3300

ggccaagcca agaaaaagaa auaaggacua gugcaucaca uuuaaaagca ucucagccua     3360

ccaugagaau aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc     3420

guugguguaa agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu     3480

uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           3594


<210>  3580
<211>  3672
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3580
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcagc cgcguucaag      120

ccaaacccga uuaauuacau acugggacuc gacaucggua uugcuagcgu uggcugggcc      180

augguggaga ucgaugaaga cgagaacccc auuugccuua ucgaucuggg gguaagggug      240

uuugaacggg cagagguccc uaaaaccgga gacuccuugg ccauggcucg ccgauuagca      300

cguucuguga gaaggcugac acggcgcaga gcccauaggc uacuccgggc ucgccgacug      360

cuuaagagag aaggcguucu gcaggccgcg gauuucgacg agaaugguuu gauaaaguca      420

cucccaaaca cucccuggca acugagggca gccgcucugg aucggaaacu cacgccuuua      480

gaguggagug ccgugcugcu ucacuugauc aagcaucgug gguaucugag ccagcgcaaa      540

aaugaaggag agaccgcaga caaggaacua ggcgcucucc ugaaaggcgu cgccgauaac      600

gcgcacgccc uucagacagg ggacuuuaga acuccagcag agcuggcuuu gaauaaguuc      660

gagaaggaau ccggacauau uaggaaccag cggggugauu acucucacac cuuuucgcga      720

aaagaccucc aagccgagcu gaucuuacug uucgaaaagc agaaagaguu uggcaauccc      780

cacgugucag ggggacucaa ggaaggcauu gagacacuuc ugaugaccca gcgcccugcu      840

uugagcggug augccguaca gaaaaugcua gggcauugua cuuucgagcc agcagaaccg      900

aaggccgcua aaaacacaua uaccgcggag agauuuaucu ggcugacgaa gcucaauaac      960

cugaggauac uugaacaagg aagugagcgg ccccugacug acaccgaacg cgcaacauug     1020

auggacgagc cuuacagaaa guccaaacuc acuuaugccc aggcuaggaa gcuguuaggc     1080

cuggaggaua ccgccuucuu uaaaggccua cguuacggga aggacaaugc agaagccucu     1140

acacucaugg agaugaaggc uuaucacgcc auuagccggg cacuggaaaa ggagggacuu     1200

aaggauaaaa aguccccauu gaaccuguca cccgaacucc aggacgagau cgguaccgcg     1260

uucagucugu uuaaaacgga ugaggacauu acuggccgac uuaaggauag aauccagccu     1320

gaaauccugg aggcuuugcu caaacauauu agcuucgaca aguucguuca aauaucucug     1380

aaggccuuaa ggcgcaucgu gccacugaug gaacagggga aacgguacga ugaggcaugc     1440

gccgagauuu acggagacca cuauggcaag aaaaauacag aagagaagau cuaccuaccc     1500

ccgauaccug cugaugaaau uagaaacccc gucgugcuca gggcccuguc ccaggcacgc     1560

aaagugauca acggugucgu ucgacgguau gggucaccag cucguaucca cauugagacc     1620

gccagagaag ugggcaagag cuuuaaggac aggaaagaga ucgagaagcg ccaagaagag     1680

aaucggaaag acagagaaaa ggcggccgcu aaauucaggg aguacuuucc uaacuucgua     1740

ggagaaccca agaguaaaga uauucuuaag uugcgacugu augagcagca gcauggcaag     1800

ugucucuacu cugggaaaga gauaaaucug ggacgcuuaa acgaaaaggg uuauguggag     1860

aucgaccacg cacugccauu uucccggacu ugggaugaca gcuucaauaa caaaguccuu     1920

guguugggcu cggaaaauca gaacaagggg aaucaaacac ccuacgagua cuuuaacgga     1980

aaagauaauu cuagagaaug gcaggaguuc aaggccaggg uugagaccuc ccguuuuccu     2040

cgcucaaaga aacagcggau ucuccugcag aaguucgacg aagauggcuu uaaagagaga     2100

aaccuaaaug acacgaggua ugugaaccga uuccugugcc aauuugucgc ugaucggaug     2160

cgccucaccg guaaggggaa aaagagagug uucgccagua auggccagau cacaaaccug     2220

cuuaggggau ucuggggcuu gcggaaggua cgagcagaaa acgacagaca ucacgcgcug     2280

gaugccguug uggucgcuug uagcacugug gccaugcagc agaaaaucac ccgcuuuguc     2340

agguacaagg agaugaaugc auucgacggg aaaacaauug auaaggagac uggagaagug     2400

uuacaucaga aaacccacuu uccacagccg ugggaguucu uugcucagga aguuaugaua     2460

cguguguucg guaagcccga cggcaagccu gaguuugaag aggccgacac accagagaaa     2520

cuccggaccc ugcuugcaga aaagcugucc ucuagacccg aggccguaca cgaauauguc     2580

acuccuuugu ucgugagcag ggcuccaaac cgcaaaaugu cagggcaggg acauauggag     2640

acgguuaaga gugcgaaacg acucgaugaa ggcguguccg uccugagagu gccccuaaca     2700

caacugaagc ucaaagaccu ggagaagaug guaaaucggg agagggaacc gaaacuuuac     2760

gaggccuuga aagcacgccu ggaagcucac aaggaugacc cugccaaagc cuuugcugag     2820

ccauucuaua aguacgauaa agcagguaac cguacccaac aggugaaggc cguuagaguc     2880

gaacaagugc agaagacugg gguguggguc cggaaucaua acggcaucgc ggacaaugcu     2940

accaugguua ggguggaugu guuugagaaa ggagacaagu auuacuuagu acccauuuac     3000

agcuggcagg ucgccaaagg cauccucccu gaucgcgcag ugguucaggg gaaggacgag     3060

gaagauuggc aacugauuga cgauucuuuc aacuuuaaau ucucacuuca ccccaaugac     3120

cugguggagg ucaucacaaa gaaggcccga auguucggau auuuugcuag uugccacaga     3180

ggcaccggua acauaaauau ccggauucau gaccuagauc acaaaaucgg gaagaacggc     3240

auucucgaag gaaucggugu gaaaacugca cugagcuucc agaaguacca gauagacgag     3300

uugggcaaag agauuaggcc augucgccug aagaagagac cucccguuag gaaacggcca     3360

gccgcgacaa agaaagcugg gcaggccaag aaaaagaagu aaggacuagu gcaucacauu     3420

uaaaagcauc ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu     3480

caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc     3540

uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga     3600

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaagaa uu                                                         3672


<210>  3581
<211>  3678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3581
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcacccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaaa guaccacguu      120

ggaauugacg uggguaccuu cagcguaggc cuggccgcga uagaggugga ugacgcuggg      180

augccaauca aaacacucuc ccuugucucu cacauucaug auucaggacu ggacccggau      240

gaaaucaaga gugccgugac uagguuggca agcuccggca uugcccggcg cacgcgacgu      300

uuauauagaa ggaagcggcg cagacugcag caacuagaca aauuuaucca gaggcagggu      360

uggcccguua uagagcucga agauuacucu gacccucugu auccauggaa ggugcgggcu      420

gagcuugcag ccucguacau cgcugaugaa aaagagcgcg gggagaagcu gucagucgcc      480

uugcgacaca uugcgagaca uaggggaugg cggaaccccu augcaaaagu gagcagucuc      540

uaccugccug acggcccauc cgaugccuuc aaggcuaucc gugaagagau uaagcgcgcc      600

ucuggccagc ccguaccuga aaccgcaaca guugggcaaa uggugacucu gugcgagcuc      660

ggaaccuuaa aacugagagg ugagggcggg guccuuagcg cuagguugca gcaguccgac      720

uaugcccggg aaauccagga gauaugucga augcaagaaa uuggacagga gcuguaccgc      780

aagaucauug augugguguu ugcggccgaa ucaccaaaag gcagugcaag cucuagaguc      840

gguaaggacc cgcuacagcc cgggaaaaau agggcucuca aggccuccga cgcuuuccag      900

cgguaccgca ucgccgcacu gaucggaaac cuuagaguua ggguggaugg cgagaaacgu      960

auucugucag uagaggaaaa gaauuuggug uuugaccacc ucgucaaccu gacaccuaag     1020

aaagagccag aaugggugac cauagccgag aucuuaggca uugaucgggg gcaacugauc     1080

ggaacugcua caaugaccga cgauggugaa cgagcgggcg caagaccccc uacgcacgac     1140

acuaauagga gcauaguuaa cagucgcauu gccccacucg uggauuggug gaagaccgcu     1200

ucugcccuug agcagcaugc aauggucaaa gcccugucca augcugaggu ggacgauuuc     1260

gacagccccg aaggggccaa gguacaggca uuuuucgcgg auuuggacga cgauguucac     1320

gcuaaacuag acucgcugca ucucccggug ggacgggccg cauauucuga ggauacacug     1380

gucagacuua cuaggcgcau gcuguccgac ggcguggauu uguacaccgc ccgacuccaa     1440

gaauuuggua ucgagccuuc auggacaccc ccaaccccuc ggaucgggga acccgucggc     1500

aacccagcug uggaccgugu ucugaagacg gugaguagau gguuagagag cgccacuaag     1560

acauggggag cacccgagag gguaauuauc gaacacgucc gcgagggcuu cgugaccgaa     1620

aaacgggcua gagagaugga uggggacaug aggcgacgcg ccgcgcggaa ugccaagcug     1680

uuucaggaga ugcaggaaaa acuaaacguu cagggaaagc cuuccagagc ugaucucugg     1740

agguaucaau cugugcagcg ucagaauugc cagugugcau acugcgguag cccaauuacu     1800

uucucaaaca gugagaugga ccacauaguc ccgcgcgccg gccaaggguc cacaaauacc     1860

cgggaaaacc ugguggcugu augucauaga ugcaaucaga gcaaaggaaa cacgcccuuu     1920

gccaucuggg caaagaacac cucuauugag ggcgugucag uuaaggaagc ggucgagagg     1980

acacgacacu gggugacuga uaccgguaug cggaguacag acuucaaaaa guucacuaaa     2040

gccguggucg agcgcuuuca gagagcuacc auggaugaag agaucgacgc caggagcaug     2100

gaauccguug cauggauggc uaaugagcuu cggucucgag uggcccagca uuucgcaucg     2160

cacgggacaa ccgugagagu auaucgcggc agcuugacug ccgaagcuag gcgugcgucc     2220

ggaaucucag gcaagcugaa auuuuucgac ggggucggaa agagucggcu cgauagaagg     2280

caucacgcca uugacgcagc ugugauagcc uuuacgucug auuacguugc cgagacacug     2340

gcugugcgca gcaaccuuaa gcaaucccag gcacaccgac aggaggcccc ucaguggaga     2400

gaguucaccg guaaagacgc ggaacaucgg gcugccugga gggucuggug ucaaaagaug     2460

gaaaaacugu cagcauugcu cacugaggau cugcgcgacg aucguguggu ugugaugagc     2520

aauguaagau uacggcuggg caacgggucu gcccacaagg aaaccaucgg aaaacuaucc     2580

aaggucaaac ucaguagcca gcugucugug uccgacauug auaaggcuuc aagugaggca     2640

cuuuggugcg ccuugacaag ggagccaggc uuugacccca aggaaggucu gccugcgaau     2700

ccagagcgcc auauccgagu gaacgggacc cacgucuaug cuggcgauaa uauuggacuc     2760

uuccccguua gcgccggcuc gaucgcacug agagggggau acgccgaauu aggcuccucu     2820

uuucaccaug cucgggugua caaaauaacu agcgguaaga aaccggccuu cgcaaugcug     2880

aggguauaua caaucgaccu uuugccuuac cgcaaccagg aucucuuuuc aguggagcug     2940

aagccacaaa cgaugaguau gagacaggcu gagaaaaagc uaagggacgc ccuggcgacc     3000

gggaaugccg aauaucucgg auggcugguc guggacgaug agcuuguugu ggacacuucc     3060

aagauugcaa cagaucaggu caaagcugug gaagccgagu ugggcaccau ccggcguugg     3120

cgcguugacg guuucuuuuc ucccagcaag cugcgauuaa gaccucucca gauguccaaa     3180

gaaggcauua agaaagaguc agcucccgag cugagcaaga ucauagauag gccagggugg     3240

cuuccugcag ugaacaagcu guucagugac ggaaauguca caguagugcg gcgcgauucu     3300

uugggcagag uuaggcucga auccacugcc caucugcccg ugaccuggaa aguccaaaag     3360

cggccagccg cuacgaaaaa ggcggggcag gccaaaaaga agaaauaagg acuagugcau     3420

cacauuuaaa agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag     3480

cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua     3540

aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa     3600

ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaagaauu                                                   3678


<210>  3582
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3582
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcagc caggauucug      120

gcguucgaca uaggaaucag cuccauuggu ugggcuuuuu cugagaacga ugaacucaag      180

gacugcggcg uucggaucuu caccaaagug gagaauccaa agacagggga aucacuugcc      240

cugccgcgcc gauuggcacg uagugccaga aagagguuag cucggcgcaa agcaagacug      300

aaccaucuaa agcaccucau ugccaaugag uuuaaacuga acuacgaaga uuaucagagc      360

uucgacgagu cccuugcuaa ggccuacaaa ggaucucuga ucucgcccua ugaguugagg      420

uuucgggcgc ucaaugaacu gcugucaaag caagauuucg cacgcguaau acuccauauc      480

gccaagcgaa gaggcuacga cgauauuaaa aacagcgacg auaaggagaa aggugcuauc      540

uuaaaggcca uuaaacagaa ugaagagaag cuggcaaacu aucagagugu gggggaguac      600

cuuuauaaag aauacuuuca gaaguucaag gaaaauucca aagaauuuac uaacgucagg      660

aauaagaaag agucuuacga acgguguauc gcucagagcu ucuugaagga cgagcugaaa      720

cuaauauuua agaagcaacg ugaguucgga uuuuccuucu cuaaaaaguu cgaagaggaa      780

gugcucagug uugccuuuua uaaacgcgcg cugaaggauu ucagccaccu ugugggcaac      840

ugcucauuuu ucacggacga gaaaagagcc ccuaaaaauu ccccacuggc auuuauguuc      900

gucgcuuuga ccaggauuau caaccuccug aauaacuuaa agaacacaga aggcauucug      960

uacacuaaag augaccucaa ugcccuucug aacgaggugu ugaagaaugg gacccuaaca     1020

uauaaacaga ccaagaaacu gcucggacug ucagacgauu acgaguuuaa gggugaaaag     1080

ggcacuuauu ucaucgaguu uaaaaaguac aaagaguuca ucaaggcucu uggggagcau     1140

aaccugagcc aggacgauuu gaaugaaauu gccaaagaca uaacacucau caaggaugag     1200

auuaaacuga agaaggcauu agccaaauau gaccugaacc aaaaucagau cgauagucua     1260

ucuaagcucg aguuuaaaga ccaccugaac auauccuuca aggcucuuaa auugguaacu     1320

ccccugaugc ucgaaggaaa gaaguacgau gaggcgugua augaacugaa ccuuaaaguu     1380

gcaauuaaug aggacaagaa agauuuucug ccugccuuca acgagacgua cuauaaggac     1440

gaagugacua auccagucgu guugcgggcu aucaaagagu accgaaaggu gcucaacgcc     1500

cuguuaaaga aauauggcaa gguccacaaa aucaauauug aacuggcacg cgagguuggu     1560

aagaaccaua gccagagagc caaaaucgaa aaggagcaga acgagaauua caaggcuaaa     1620

aaggacgccg aacuagagug cgaaaaacuc gggcugaaga uuaacucgaa aaauauacuu     1680

aaguugaggc uguucaaaga gcaaaaggaa uuuugugcau auucuggaga gaagaucaaa     1740

auuuccgauc uccaggacga gaagaugcug gaaaucgauc acaucuaccc cuauucacgg     1800

aguuucgacg auagcuacau gaacaaagug uuaguauuua ccaagcagaa ucaagagaaa     1860

cugaaccaga caccuuucga agcguuuggc aaugacuccg cuaaguggca gaagauugag     1920

gugcuugcca aaaacuugcc aacuaagaaa cagaagcgca uacucgauaa aaauuacaag     1980

gacaaggaac aaaagaacuu caaggauaga aaucugaacg acaccaggua uaucgcacgu     2040

cuaguccuga auuacacaaa agauuaucuc gacuuucugc cgcuuucuga ugacgagaac     2100

accaaguuga augacacgca gaaaggcagc aaggugcaug uugaggccaa gucagggaug     2160

cugaccagug cuuuacggca cacaugggga uucuccgcca aagaucgaaa caaccaccuc     2220

caucacgcaa uugacgcugu gaucauugcc uacgcgaaua acagcaucgu caaggccuuu     2280

ucugauuuca aaaaggaaca ggagucaaau agugcugaac uguaugccaa aaagauaagc     2340

gagcuugacu acaagaacaa aagaaaguuu uucgagcccu uuuccgguuu caggcagaaa     2400

gugcuggaua agaucgacga aauuuucgua ucuaaaccug agcgcaagaa accaucgggc     2460

gcauugcaug aagagaccuu ucggaaggaa gaggaguucu aucaaagcua cgggggaaag     2520

gaaggcguuc ucaaagcucu ggagcuaggu aagaucagaa aagugaaugg gaagauuguc     2580

aaaaacggcg auauguuuag gguggacauc uucaagcaca agaaaacuaa uaaguuuuac     2640

gccgucccca uauauacaau ggauuucgca cugaaagugc ucccuaacaa ggcgguugcc     2700

cgcuccaaaa agggagaaau uaaggacugg auccugaugg augagaauua cgaauuuugc     2760

uucucacuuu auaaagacag uuugauucug auccagacca aggauaugca ggagccagag     2820

uuuguguacu auaacgcuuu cacgucuagc accguauccu uaauagucuc aaaacaugac     2880

aauaaguuug aaacacucag caaaaaccag aagauccugu ucaagaaugc caacgagaaa     2940

gaagugauug caaagucuau cggcauucaa aaucuuaaag uuuucgagaa guacaucgug     3000

uccgcucugg gggaagucac uaaagccgag uuucgacagc gggaggacuu caagaaaaag     3060

cgucccgcag ccaccaagaa agcuggacag gcgaagaaaa agaaauaagg acuagugcau     3120

cacauuuaaa agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag     3180

cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua     3240

aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa     3300

ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3360

aaaaaaaaaa aaagaauu                                                   3378


<210>  3583
<211>  3789
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3583
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaag cgaccugguu      120

cucggacuug auauugguau aggcuccgug gggguaggaa uccugaacaa ggugaccggc      180

gagauuaucc auaaaaauuc uaggauuuuc ccagccgcgc aggcugaaaa caauuugguc      240

cggcgcacaa accgacaagg ucguagauua gccaggcgga agaagcaccg cagagugagg      300

cugaaucggc uauuugagga aucagggcuc aucacugacu ucacgaaaau aaguaucaac      360

cugaauccgu accagcuucg cguuaaggga cugaccgaug aguugagcaa cgaagaacuc      420

uuuauugcac ugaaaaauau ggugaagcau cgaggcaucu ccuaucugga cgaugccucu      480

gacgauggca acucgucagu cggggacuac gcucagauug ugaaagagaa uagcaagcag      540

cucgaaacaa agacucccgg acaaauccag uuagagagau aucagaccua cggucagcug      600

aggggcgauu ucacaguaga aaaagacggg aagaaacacc ggcuuauaaa cguuuuuccu      660

accagugcau aucguuccga ggccuugcgc auucugcaaa cucagcagga guucaaucca      720

cagaucacag augaauuuau uaacagauac cuagagaucc ucaccggaaa gaggaaauau      780

uaccauggcc ccgguaauga aaagucucgg acggacuacg ggcgauaucg cacuagcgga      840

gagacccugg auaacaucuu cggcauucuu auaggcaaau gcacauuuua cccugacgag      900

uucagagcug ccaaggcguc cuauacugca caagaguuua accuguugaa ugaccucaac      960

aaucugaccg ugccaacaga gaccaagaaa uuaucaaagg aacagaaaaa ccagaucauu     1020

aauuacguca agaacgagaa agccaugggg cccgcuaagc uguucaagua uaucgccaaa     1080

cuccuuaguu gugauguggc agacauaaag ggauacagga uugauaaaag cgguaaggcu     1140

gaaauccaca cguuugaggc cuaucggaaa augaagacuc uggaaacauu ggacaucgag     1200

caaauggauc gcgagacccu agacaagcug gcguacgugc ucacucugaa uacagaaaga     1260

gagggcauuc aggaagcccu ugagcacgag uucgcagaug ggucuuucuc ccagaaacag     1320

gucgacgagc ugguucaauu uaggaaggcu aacucaagca ucuucggaaa aggcuggcau     1380

aauuuuagug ugaaguugau gauggagcuc auuccugaac uguacgagac cucugaagaa     1440

cagaugacga uauuaacccg ucuggguaaa cagaagacaa cuuccagcuc gaacaagacc     1500

aaauauaucg augagaagcu acucacagaa gagauuuaca auccaguagu ggccaaaucu     1560

guccggcagg cuaucaagau cgugaacgcc gcaauuaaag aauaugggga cuucgauaau     1620

auaguuaucg agauggcccg agaaacuaac gaggacgacg agaagaaagc uauucaaaag     1680

auccagaagg cgaauaaaga ugaaaaggac gcagccaugc ugaaagcugc caaccaguac     1740

aacggcaagg cagagcuucc gcacuccgug uuucauggac acaaacaguu ggccaccaag     1800

auuagacugu ggcaccaaca gggcgaaagg ugccucuaua cagggaagac caucucaaua     1860

caugaucuga ucaauaacag uaaucaguuc gaggucgacc acauucuucc ccugagcauc     1920

acuuuugaug acuccuuggc uaacaaagug cucguauacg ccacggcaaa ucaggaaaag     1980

ggacaacgca caccuuauca ggcgcuggau ucuauggacg augcuuggag cuuccgggag     2040

uuaaaagccu uuguuagaga gucaaagacc cugaguaaca aaaagaagga auaccuacuc     2100

acugaggaag acauuuccaa auucgaugug aggaagaaau uuaucgagcg caaucugguc     2160

gacacccgau acgcaagccg ggugguccuu aacgccuugc aggaacauuu ccgugcucac     2220

aagauagaua caaaaguguc uguugugaga ggucaauuua ccucacagcu gaggcgccau     2280

uggggcauug agaagacucg ggacacauau caccaccaug ccguagacgc acucaucauu     2340

gcugccagua gccagcugaa uuuauggaag aaacagaaga acacgcuggu cuccuacucu     2400

gaggaucaac uuuuggacau cgaaaccggg gagcucauau cggaugacga auauaaagag     2460

agcguguuca aggcgccaua ccagcacuuc guugauacuc ugaaauccaa ggaguuugaa     2520

gacucaaucc uauucaguua ucagguggau ucuaaauuua auagaaagau uagcgacgcc     2580

acaaucuacg cuaccaggca ggcaaagguc ggaaaagaua aggccgacga gacauaugug     2640

cugggcaaaa uuaaggauau cuacacucaa gacgguuacg augcuuucau gaaaauauau     2700

aagaaggaca aauccaaguu ucucauguac cgacaugacc cccagaccuu cgaaaaagua     2760

auugagccua uccuggaaaa cuauccaaau aagcagauca acgagaaagg gaaggaggug     2820

cccuguaauc cguuucuuaa guacaaagag gaacacggcu auauucgcaa guacagcaaa     2880

aagggaaacg gcccugaaau caaaucauug aaguauuacg auucuaagcu ggggaaccac     2940

auugacauaa cgcccaaaga uuccaauaac aagguugucu uacagagugu gagcccaugg     3000

cgggccgacg uguacuucaa uaaaaccaca ggaaaguaug agauccucgg ucugaaauac     3060

gcagaucuuc aauuugaaaa gggcacuggg accuauaaga uuucucagga gaaauacaac     3120

gacaucaaga aaaaggaggg agucgauucc gacucagagu ucaaauuuac acuguauaag     3180

aaugauuugc uccugguuaa agacacugaa accaaggaac agcaacuauu cagauuucug     3240

aguaggacga ugccuaagca gaaacauuac guggagcuca agcccuauga uaaacagaag     3300

uucgaaggcg gugaggcgcu gauuaaagug cuugggaacg uagccaauag cggccagugc     3360

aagaagggau ugggcaaauc gaacaucucc auauacaagg uccguacaga cgugcugggg     3420

aaucaacaca ucauuaaaaa cgagggagac aagccaaaau uagauuucaa gcgccccgcu     3480

gccaccaaga aagcaggcca ggcuaagaaa aagaaauaag gacuagugca ucacauuuaa     3540

aagcaucuca gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau     3600

cucuuuuucu uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu     3660

aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3780

aaaagaauu                                                             3789


<210>  3584
<211>  4347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3584
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaac ccaguucgag      120

ggauuuacaa accuguacca aguuagcaag acucucaggu ucgaacuuau uccacagggu      180

aaaacgcuga agcauauaca ggagcagggc uuuaucgaag aggacaaggc ccggaaugau      240

cacuauaaag aauugaagcc gauuaucgac cgcauuuaca aaaccuaugc ggaucaaugc      300

uuacagcugg ugcagcuaga cugggagaac cucuccgcug ccaucgauuc uuaccgaaag      360

gagaaaacag aagagacucg uaaugcacug auagaggaac aggccaccua uagaaacgcu      420

auccaugacu acuucauugg gaggacagau aaucuuaccg acgcaaucaa caagcggcac      480

gccgagauuu auaagggacu guuuaaagcu gaauuguuca auggcaaggu acucaaacaa      540

cuggguacug ugacaaccac ggagcaugaa aacgcccugc uccgcucauu ugauaaguuc      600

acuaccuacu uuaguggguu cuacgagaau aggaaaaacg ucuuuagcgc ggaagacauc      660

uccacagcaa uaccccacag aauugugcag gauaauuucc cuaaguuuaa agagaacugu      720

cacaucuuca cucgguuaau uaccgccguu ccaucucugc gcgagcauuu cgaaaaugug      780

aagaaggcua ucggaaucuu ugucucgaca ucaauugagg aaguguucag cuuucccuuc      840

uauaaccagc uuuugaccca gacgcaaaua gaccuguaca aucagcuacu cggcggcauc      900

agucgagagg ccgggacuga gaaaauuaag ggacugaacg aaguacuuaa ccuggcaauc      960

cagaaaaaug augagacagc ucacauaauu gccuccuugc cucauagauu uaucccacuc     1020

uucaagcaaa uccugucuga caggaacacc cuuagcuuua uucuggaaga guucaaaucc     1080

gacgaagagg uuauccaguc auuuugcaag uauaagacuc uccuucggaa ugaaaacgug     1140

cuggagacag cggaggccuu guucaaugaa cuaaacagua uugaucugac ccacauauuu     1200

aucagccaca aaaagcucga gacgauuucu uccgcacugu gugaccauug ggauacccuu     1260

cguaaugcuc uguacgaacg cagaaucuca gaguugacag guaaaaucac uaagagcgcc     1320

aaagagaagg uccagaggag ucucaagcac gaagacauua accugcagga gauaaucucu     1380

gcugccggca aagaauuauc cgaggcauuc aagcagaaaa ccagcgaaau ucugucgcau     1440

gcccacgcug cgcuagauca accccucccu acaacucuga agaaacagga ggagaaggaa     1500

auccuuaagu cucaguugga cucccugcuc gggcuguauc aucuucugga uugguuugca     1560

guggacgagu caaaugaagu ggauccagag uucagugccc gguugaccgg aauuaaacuc     1620

gaaauggagc cgagccuguc cuucuacaac aaggcucgaa auuaugccac uaaaaagccc     1680

uacucugucg agaaauuuaa guuaaacuuc caaaugccua cccuggcaag cggcugggac     1740

guuaauaaag aaaagaacaa uggugccauc cuauuuguga agaacgggcu cuacuaucug     1800

ggaauaaugc caaaacagaa gggccgcuac aaagcucuuu cauucgagcc cacugaaaag     1860

acgagugagg gcuuugauaa aauguauuac gacuauuucc cugacgccgc aaagaugauc     1920

ccaaagugcu ccacacaguu gaaagcggua accgcucacu uucagacuca caccacaccc     1980

auucugcuca gcaacaauuu caucgaaccg cuggagauua ccaaggagau cuacgauuua     2040

aacaauccug aaaaagagcc caagaaauuu caaacugccu augcaaagaa gacaggggac     2100

cagaaaggau acagagaagc ccuguguaag uggauagauu ucacgaggga cuuucuuucu     2160

aaauacacca agacuacauc aauugauuug aguagccucc ggccauccuc ucaguauaaa     2220

gaccugggug aguacuaugc ugagcuaaac ccucugcucu accauaucuc guuccagcgc     2280

auugccgaaa aggagaucau ggaugcagug gaaaccggca agcuguaucu uuuucaaaua     2340

uacaauaaag acuucgcuaa ggggcaccau ggaaaaccca acuugcacac acuguauugg     2400

acuggcuuau ucagcccaga gaaucucgcc aagaccucca ucaaacugaa cggucaggcg     2460

gaauuauuuu acagacccaa gucaaggaug aaacguaugg cccaccggcu gggggagaag     2520

auguugaaua agaaacucaa ggaucagaaa acgccuauuc cagacacccu guaccaagag     2580

cuauaugauu acgucaacca ucgacugagu cacgaccucu cugaugaagc uagagcacug     2640

cuuccgaaug ugaucacaaa ggagguuagc caugaaauua ucaaagacag gcgcuucacu     2700

uccgacaagu uuuucuuuca cgugcccaua accuugaacu aucaggccgc uaauucaccu     2760

agcaaguuca accagcgggu caaugccuac cugaaagagc auccagaaac acccauuauc     2820

ggcaucgaua gaggagagag gaacuuaauu uauaucacug ugauugacuc uaccggcaag     2880

auacucgagc agcgcucccu gaacacgauc caacaguuug auuaccagaa aaagcuggac     2940

aaucgagaaa aagagcgggu agcagcgcgu caggccugga guguuguggg gacaauuaag     3000

gaucuuaagc aaggauaucu aagccagguc auccacgaaa uuguggaccu caugauccac     3060

uaccaggcug ucgugguucu ggagaacuug aauuucgguu uuaaaucuaa gagaaccggc     3120

auagccgaaa aagcagugua ccagcaauuc gagaagaugc ugaucgauaa auuaaacugc     3180

cucguacuga aggacuaucc ugcugagaag gucgggggag ugcuuaaucc auaccagcug     3240

acugaucagu uuacauccuu cgccaaaaug ggcacccaau cagguuucuu guuuuauguu     3300

cccgcaccgu acacaaguaa gauugacccu cucaccgggu ucguggaucc auuugucugg     3360

aaaacaauca agaaccauga aagcaggaaa cacuuccugg agggcuuuga cuuccuacau     3420

uaugauguga agacgggaga cuuuauucug cacuucaaga ugaaucgcaa ccuuucguuu     3480

cagcggggcc uccccggguu caugccugcc ugggacaucg uauuugagaa aaaugaaacc     3540

caguucgaug cuaagggaac ucccuuuaua gcggguaaaa gaauugugcc aguuaucgag     3600

aaccauaggu ucacaggccg auaccgcgac cuguauccug ccaaugaauu gaucgcacug     3660

uuagaggaaa aggggauugu cuuccgggau ggauccaaca uacuccccaa acugcuugag     3720

aaugacgauu cucacgcuau cgacaccaug guggcccuga uuagaagcgu guugcagaug     3780

aggaacucaa augccgcuac aggcgaagau uacaucaaca guccaguccg ugaccucaac     3840

gguguuuguu uugauucccg cuuccaaaau ccggaguggc cuauggacgc agaugccaac     3900

ggggcguacc acauugcucu gaagggccag cuacugcuua aucaucucaa agagucuaag     3960

gaccugaagu ugcagaacgg aaucagcaau caggacuggu uagccuauau acaagaacug     4020

cggaacaaaa gacccgcagc cacuaagaaa gcuggccagg caaagaaaaa gaaguaagga     4080

cuagugcauc acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa     4140

gaucaauagc uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua     4200

aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa     4260

uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aagaauu                                         4347


<210>  3585
<211>  4326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3585
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcaag cauuuaccag      120

gaguucguua acaaguauuc ccugucuaaa acccucaggu uugaacuuau accacaagga      180

aagacacugg agaauaucaa ggcccggggu uugauuuuag acgaugaaaa acgcgcgaag      240

gacuacaaaa aggcuaaaca gaucauugau aaguaucauc aguucuuuau cgaggaaaua      300

cugucaagug ugugcaucag cgaggaccua cuccagaacu acuccgaugu auauuucaag      360

cugaaaaagu cugacgauga caaucuucaa aaagauuuua agucggccaa agacacuauu      420

aagaaacaga ucucagagua cauuaaggau agcgaaaagu ucaaaaaccu guuuaaucag      480

aacuugaucg acgcaaagaa aggccaggag agugaucuca uacuguggcu gaagcaaucc      540

aaagacaaug ggauugaacu cuucaaggcc aacucugaca ucacggauau ugacgaggcu      600

uuagagauca ucaagagcuu uaaaggaugg accacauauu ucaagggcuu ucacgaaaau      660

cguaaaaacg uguacuccuc aaaugauauu ccgacaagua uaaucuaccg aauugucgac      720

gauaaccugc ccaaguuccu ugagaauaaa gcaaaguaug agagcuugaa ggacaaagcc      780

ccugaggcua ucaacuacga acagauaaag aaagaucugg ccgaggagcu aaccuuugac      840

auugauuaua agacaucuga agugaaucag agaguuuucu cccucgacga gguguucgaa      900

aucgcgaacu uuaacaauua ccugaaccag ucagguauca ccaaauucaa uacuauuauc      960

gggggaaagu uugucaacgg cgagaauaca aagaggaaag gcauuaacga auauauaaau     1020

cuuuacagcc aacagaucaa cgauaagacc cugaaaaagu auaaaaugag uguguuguuc     1080

aagcagauuc ucucugacac ggaguccaag agcuuuguaa ucgacaaacu ggaggaugac     1140

ucggauguug ugacuaccau gcaaucuuuc uacgaacaga ucgcagccuu uaagacaguc     1200

gaggaaaaau ccauuaagga gacuuuauca cuccuguucg acgaucuuaa agcucagaag     1260

cuggacuuga guaaaauaua cuuuaagaau gauaagagcc uaaccgaccu gucccagcaa     1320

guguucgaug acuauucugu gaucgggaca gccguccucg aauacauuac ccagcagauc     1380

gcacccaaaa accuggauaa uccaagcaag aaagagcagg agcuuauugc uaagaaaacg     1440

gaaaaggcca aguaucuguc auuggagacu aucaaacucg cgcuggaaga guuuaacaag     1500

caucgggaca uagauaaaca augucgcuuc gaggaaaucu uagccaauuu ugcagcuauu     1560

ccuaugaucu ucgacgagau ugcccagaac aaggacaauc uggcucagau caguauaaaa     1620

uaccagaacc aaggaaagaa ggaucuacuc caggccuccg cagaagacga uguuaaagcc     1680

auuaaggacc ugcuugauca gacaaacaau uugcugcaca aacucaagau cuuccauauu     1740

agccagucug aggacaaagc uaacauccug gauaaggacg aacacuuuua ucuuguguuc     1800

gaggagugcu acuuugaacu ggcgaauaua guaccauugu auaacaaaau cagaaauuac     1860

auuacccaaa agcccuacuc agaugagaag uucaaacuca acuuugaaaa uaguacucug     1920

gcaaacgguu gggacaagaa uaaagagccu gauaacacag ccaucuuauu cauuaaggac     1980

gauaaauauu accugggcgu gaugaauaag aagaacaaua aaaucuuuga cgacaaggcu     2040

auaaaagaaa acaaggggga gggauauaaa aagauugucu acaagcuacu cccaggcgcc     2100

aauaaaaugc ugccgaaggu guucuuuagc gcaaaaucca ucaaguucua uaaccccucu     2160

gaggauaucc uuaggauucg gaaccacucg acccauacga aaaaugguag cccucagaag     2220

ggguacgaaa aguuugaguu caacaucgaa gacugucgca aauuuauuga uuucuauaag     2280

caguccauau caaaacaccc agaguggaag gacuucggau uucgauucag ugauacccaa     2340

agauacaauu cuaucgacga auuuuacagg gagguugaga accagggcua uaaauugaca     2400

uucgaaaaua uuagcgaguc cuacaucgau ucagugguca accagggcaa gcuguaucuc     2460

uuucagauuu acaauaagga cuucagcgcc uauucuaaag ggcggcccaa ccugcauacu     2520

uuauacugga aggcucuguu ugaugaacgu aaucuucaag acgugguaua uaaauugaac     2580

ggagaggccg aacucuucua ccgcaagcag uccaucccua aaaagauaac ccacccagca     2640

aaagaagcga ucgcuaauaa gaacaaggau aaucccaaaa aggagagugu uuuugaauac     2700

gaccugauua aagacaagag auucacagag gauaaauuuu ucuuccacug cccgaucacu     2760

auuaacuuua agagcucugg ugccaauaag uucaacgacg aaaucaaccu acugcucaaa     2820

gagaaggcaa augaugugca uauacugucc auugacaggg gcgagcggca ccuugccuau     2880

uacaccuugg ucgaugggaa aggaaacauc aucaagcagg acacauuuaa uauuauaggc     2940

aacgaucgaa ugaaaaccaa uuaucaugac aagcuggcug ccaucgaaaa ggaucgcgac     3000

ucagcaagaa aagauuggaa gaaaauuaac aauaucaagg agaugaaaga agguuacuua     3060

agucaggugg uccacgagau ugcuaagcuc gugaucgaau auaacgccau aguuguguuc     3120

gaggaccuga auuuuggguu caagaggggc cgguuuaaag uagagaagca agucuaccag     3180

aaacuugaaa agaugcugau cgagaaauug aacuaucucg uguucaagga uaaugaauuu     3240

gacaaaacug gaggcguucu gcgcgcguac cagcuaacgg ccccuuucga gacauuuaag     3300

aagaugggga aacagaccgg aauuaucuac uaugugcccg cugguuucac uagcaagauu     3360

uguccaguca ccggcuuugu gaaccaacug uacccuaaau augaaucggu auccaagucu     3420

caggaguucu ucagcaaauu ugacaagauc ugcuacaauc ucgauaaggg guauuucgag     3480

uuuucauucg acuacaaaaa cuuuggagau aaggcagcca aaggcaagug gacaauagcu     3540

aguuucgguu ccagacugau uaauuuuagg aacucugaca aaaaucauaa cugggauacc     3600

cgugaagugu aucccacuaa ggagcuugaa aaguugcuga aagacuacag caucgaguac     3660

gggcacggcg aguguaucaa ggccgcaauu ugcggagaau ccgauaaaaa guucuuugcg     3720

aaauuaacau caguucucaa cacgauccug cagaugcgga auagcaagac cggcacugag     3780

cuugacuauc ugauaagucc agucgccgau gugaacggga auuucuuuga cucucgacaa     3840

gcucccaaga acaugccuca ggaugccgac gcaaauggag cuuaccacau uggucuaaaa     3900

ggccucaugc uguuggggag aaucaagaac aaucaggaag gcaaaaagcu gaacuuagug     3960

auuaaaaaug aggaauauuu cgaguuuguc cagaacagga auaacaagcg cccagccgca     4020

acuaaaaagg ccggacaagc uaagaaaaag aaauaaggac uagugcauca cauuuaaaag     4080

caucucagcc uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc     4140

uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau     4200

cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

agaauu                                                                4326


<210>  3586
<211>  4582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3586
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcagacaag aaauacagca      120

uuggacugga uauagguaca aacuccguug gcugggccgu gaucacagac gaguauaagg      180

uaccaucuaa gaaauucaag gugcucggga auacugauag gcauucaauu aaaaagaacc      240

uuaucggagc gcuguuguuu gacaguggcg aaacggcuga ggccacccgg uuaaaacgca      300

cagcacgacg uagauacacu aggcggaaga aucgcauuug cuaucugcag gaaaucuuca      360

gcaacgagau ggccaagguc gaugacuccu uuuuccacag acuagaagag ucuuuucucg      420

uggaggaaga uaaaaagcau gagaggcacc cgauauucgg uaauaucguu gacgaagugg      480

cuuaccauga gaaauauccc accauuuacc aucugcggaa gaaacuuguc gauucgacag      540

acaaggcaga ucugcgcuug aucuaucucg cccuggcuca caugauuaaa uuucgagggc      600

acuuccugau cgagggagac cucaacccug auaauucaga cguggacaag uuauuuauac      660

aacugguaca gaccuacaac cagcuuuucg aagagaaucc aauuaacgcc agcggcguug      720

augcgaaggc aaucuugagu gccagacugu ccaaaucuag gcggcuagaa aaucucauug      780

cucagcugcc cggcgagaag aaaaacgggc uuuuuggaaa ucugaucgcc uugagccucg      840

gucugacucc uaacuucaag uccaauuuug acuuagcaga agaugcuaaa cugcaacucu      900

caaaggacac auacgaugac gaucuugaca accuguuggc ccagaucggc gaucaguaug      960

cggaccuauu ccuggccgca aagaaccuca gugaugcuau ucugcuuagc gacauacugc     1020

gugugaauac cgagaucacg aaagccccau ugucugcuuc caugauuaag cgcuacgacg     1080

agcaccauca ggaucucacu cuguuaaaag cccuggucag acaacagcua cccgaaaagu     1140

auaaagagau cuucuuugac cagucaaaga acggguacgc aggauauaua gauggcggug     1200

ccagccagga agaguucuac aaguuuauua aaccuauccu cgaaaagaug gacgggaccg     1260

aggagcugcu ugugaaauug aauagggaag aucugcuccg gaagcaacga acauucgaca     1320

acggaaguau cccacaccag auucaccugg gcgagcuuca ugcuauccug cgcagacagg     1380

aagauuuuua uccguucuug aaagacaaua gggagaagau ugaaaagaua cucacuuuuc     1440

ggauccccua cuacgugggc ccucuggcgc gcgggaacuc uagauucgca uggaugacca     1500

ggaaauccga ggagacaauu accccaugga auuuugaaga ggucguugau aagggagcca     1560

gcgcucaauc guucaucgaa cguaugacga acuuugacaa aaauuuaccc aacgagaagg     1620

ugcugccuaa acacucucua cucuaugagu acuucacugu auauaaugaa cugacaaagg     1680

ugaaauacgu caccgagggu augcggaagc cagccuuucu uuccggcgaa cagaagaaag     1740

caaucgugga uuugcuguuc aagacuaacc gaaaaguuac agugaagcag cucaaagagg     1800

acuauuucaa gaagauugaa uguuuugauu cagucgagau aaguggggug gaggacagau     1860

ucaaugccag ccugggaacc uaccaugacu uacugaaaau cauuaaggau aaagacuuuc     1920

uugauaacga agagaaugaa gacaucuugg aggauauugu acucacgcug acccuauucg     1980

aagacaggga gaugaucgag gaacgccuga agacauaugc ucaccucuuu gaugacaaag     2040

uuaugaagca gcugaagcgg agaagguaca cuggcugggg ucgccuuucc cgaaaauuga     2100

uaaacgggau ccgggauaag caaucuggca aaaccauucu ggacuucuua aagagcgaug     2160

gauuugccaa ccguaauuuc augcagcuca uccaugacga uucacugaca uuuaaagagg     2220

acauucagaa ggcacaggug aguggccaag gggacucccu ucacgaacac aucgcgaacc     2280

uggcuggaag ccccgccaua aagaaaggua uuuugcagac ugucaaggug gucgaugagc     2340

ucgugaaagu uaugggcaga cauaagccgg aaaauaucgu gauugagaug gcaagggaga     2400

accagaccac acagaaaggg caaaagaacu cucgcgaacg gaugaaaaga aucgaggaag     2460

gaauaaagga gcugggcuca cagauccuaa aggagcaccc uguagaaaau acccagcugc     2520

agaaugagaa acucuaccug uauuaccuuc aaaacgguag ggacauguau gucgaucagg     2580

aauuggacau uaaucgacug agugauuacg acguggauca uaucguuccc cagagcuucu     2640

uaaaggacga uuccauugac aacaaagugc ucacucgcuc ugauaagaau cgggggaaau     2700

cggacaacgu cccaagcgag gaagugguaa agaagaugaa aaauuauugg agacaacugc     2760

uuaacgccaa gcugaucacg cagaggaaau uugacaaucu aacaaaggcu gagcguggcg     2820

gacucuccga gcuggauaaa gccggcuuca uaaagcgcca guugguggaa acccggcaga     2880

uuacuaagca cguugcacaa auccuggacu caagaaugaa caccaaauac gaugagaacg     2940

acaaguuaau cagggaaguc aaagugauua cacucaagag uaaacuggug ucugauuuuc     3000

gaaaggacuu ccaguucuau aagguccggg agaucaauaa cuaccaccau gcucacgaug     3060

ccuaccuuaa ugcgguugug gggaccgccc ugauuaaaaa guauccuaaa uuggaaagcg     3120

aguuugugua cggagacuau aagguauacg auguccgcaa aaugauagcu aaguccgagc     3180

aggaaaucgg uaaggcaacu gccaaauauu ucuuuuacuc aaacauuaug aauuucuuua     3240

agacagagau cacgcucgcu aacggcgaaa uuagaaaaag gccccugauc gagaccaaug     3300

gggaaacugg agagauagug ugggacaagg gccgggauuu cgccacaguu cgaaaagugc     3360

uaagcaugcc acaggucaac aucgugaaga aaaccgaggu ucaaacaggu ggguuuucua     3420

aggaauccau ucugcccaag agaaauagug acaaacuuau cgcacgcaag aaagauuggg     3480

acccuaagaa auauggcgga uucgacagcc caacuguggc guacucugua cucgucgugg     3540

ccaaggugga gaagggcaaa uccaagaaac ugaagucagu caaagaauug cuggggauua     3600

ccaucaugga gaggaguagc uuugagaaga acccgauaga uuucuuagaa gcuaagggau     3660

acaaagaggu uaagaaagac cucauuauca agcugcccaa auauucgcuu uuugaacugg     3720

agaauggccg uaagcggaug uuggccuccg cuggugaacu ccagaagggg aacgagcugg     3780

cacuaccuuc uaaauacgug aauuuccugu aucuugccag ccauuacgag aagcucaaag     3840

gaucaccaga agauaacgag cagaagcagc uguucguaga acaacacaaa cauuauuugg     3900

acgagaucau ugaacagaua agugaguuuu ccaagagagu gaucuuagca gaugccaauc     3960

uggacaaagu ccucucugcu uacaacaagc acagggauaa gcccauucgc gagcaggcgg     4020

aaaacaucau ucaccuguuc acgcuuacca aucugggcgc cccugcagcu uuuaaauauu     4080

ucgacacaac uaucgaucga aagagauaca ccagcacaaa agagguguug gacgccacuc     4140

uaauacauca auccaucacc ggucuguacg aaacgcggau ugaucucuca cagcugggcg     4200

gggacaagag gccagccgcu accaaaaagg caggacaggc caagaaaaag aaaggacuag     4260

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     4320

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     4380

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     4440

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     4560

ucuuuucaga gccaccagaa uu                                              4582


<210>  3587
<211>  5365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3587
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaacuuc aagauucugc      120

caauagccau cgaccucgga guuaaaaaua ccgguguguu uagcgcguuc uaccagaagg      180

gcacaucccu ugagaggcug gauaacaaga augggaaagu auaugaauug ucuaaggacu      240

cauacacuuu acugaugaac aaucggacgg cucgccgaca ucaacguaga ggaauugaua      300

ggaaacagcu agugaagcgg cucuuuaaac ugaucuggac cgagcagcuu aaccuggaau      360

gggacaagga uacacagcaa gccauuaguu ucuuguuuaa ucgcagaggc uucagcuuua      420

ucacugacgg uuauuccccg gaguaccuca acauaguccc cgaacaggug aaggcaaucc      480

ugauggauau uuucgacgau uauaaugggg aggacgaucu ggacucuuac cucaaauuag      540

ccaccgagca ggaaucgaag aucucagaga uuuauaacaa acugaugcag aagauccuug      600

aauuuaaauu gaugaagcug ugcacagaua uaaaagacga caagguuagc accaagacuc      660

uaaaagagau uacaaguuac gaguucgaac uccuggcuga uuaccuugca aauuauuccg      720

agucucugaa gacccaaaaa uuuagcuaca cggacaagca gggaaacuug aaagaacucu      780

ccuauuacca ccaugauaag uauaauaucc aggaguuccu gaagaggcac gccacuauua      840

acgaccggau cuuagauacc cugcucacag acgaucuuga caucuggaau uuuaacuucg      900

aaaaauucga uuuugacaag aacgaggaga aacugcagaa ucaagaagau aaggaccaua      960

uucaggcuca cuugcaccau uucguguuug ccgucaacaa aauaaaguca gagauggcga     1020

guggcggccg ccaccgaagc caguacuucc aagaaaucac uaaugugcua gacgagaaca     1080

aucaucagga aggguaucug aagaacuuuu gugagaaucu ccacaacaaa aaguacucua     1140

aucuguccgu uaaaaaccuu guaaaucuga uuggaaacuu gucaaaucuc gagcugaagc     1200

cuuuaagaaa auacuucaac gauaagaucc acgcaaaggc cgaccauugg gaugaacaga     1260

aauuuaccga gacauauugc cacuggauac ugggugaaug gagggugggc gucaaggacc     1320

aggauaaaaa ggacggggcu aaauacagcu auaaggaucu auguaaugag cucaaacaaa     1380

aggugaccaa ggccggacug guggacuucc uuuuggaacu ggauccaugc cggacgauuc     1440

ccccuuaccu cgacaacaac aaucguaaac cacccaagug ucagagucug auccuuaacc     1500

cuaaauuucu ggauaaucag uauccaaacu ggcagcaaua cuugcaggag cucaagaaac     1560

ugcagucuau ccagaauuau uuagacuccu ucgagacuga ucugaagguc cuaaagagcu     1620

cgaaagacca accguacuuu guugaauaca agucuuccaa ccagcagauu gcaucaggcc     1680

agcgcgacua uaaagaucuc gacgcuagaa uccugcaauu cauuuuugau agggugaagg     1740

ccagugacga gcuuuugcug aaugaaauau acuuccaggc gaaaaagcuc aagcagaaag     1800

ccagcuccga gcuggagaag uuagaaucua gcaaaaagcu ggaugaggua aucgcaaacu     1860

cacaacuuag ucagauuuug aaaucccagc auacaaaugg uaucuucgaa caggggaccu     1920

uucuccaccu ggugugcaag uauuacaagc aacggcagcg agcucgcgac agcagacuau     1980

auaucaugcc cgaguacagg uaugauaaaa agcugcauaa auacaacaau acuggacggu     2040

ucgacgauga caaccagcuc cugacauacu guaaucacaa gccucgccag aaaagauauc     2100

aacuuuugaa cgaucuggcc ggcgucuuac aggugucucc aaauuuucuc aaggacaaaa     2160

uuggcucaga ugacgaccug uucauaagua aguggcuugu ugaacacauc aggggguuua     2220

agaaagcaug cgaggauagc cugaagauuc agaaagacaa ccguggauug cucaaccaua     2280

agaucaauau ugcccggaac accaaaggua agugugagaa ggaaaucuuc aaucugauau     2340

gcaaaaucga gggcuccgaa gauaagaaag ggaacuacaa gcacggacua gcauaugagc     2400

ugggcgugcu ccuguuuggu gaacccaaug aggccucuaa accugaguuc gaccgaaaga     2460

uuaagaaauu uaacucgauc uacagcuucg cucagauuca acagaucgcg uuugcagaaa     2520

gaaaggggaa ugccaacacg ugugcugucu gcuccgccga uaaugcacau aggaugcagc     2580

aaauaaaaau uaccgagcca guggaagaca acaaggauaa aaucauucuu ucagccaagg     2640

cucagcgcuu gcccgccauc ccgacacgga uaguagacgg cgcaguuaag aaaauggcga     2700

cuauccuggc uaagaauauu guggaugaca acuggcagaa uaucaaacag gucuuaagug     2760

ccaagcacca acuccacauu ccuaucauaa ccgagucuaa cgcauucgaa uuugagcccg     2820

cccuggcuga ugugaaagga aagagccuua aggacagaag gaaaaaggcc cuggagcgca     2880

uuuccccaga aaauaucuuc aaagacaaga acaaccgaau caaagaguuc gcaaagggca     2940

uuucagcuua uagcggggcc aaucuaacag auggagacuu ugauggugcg aaagaagaac     3000

ucgaccauau cauuccucgg ucucacaaga aguacggcac ucugaacgau gaggccaauu     3060

ugauaugcgu cacccguggg gacaacaaaa auaagggaaa cagaaucuuc ugucugaggg     3120

auuuagcuga caauuauaaa cucaagcagu uugaaacaac cgaugaccug gagauugaga     3180

aaaagaucgc agauacuauu ugggacgcca acaagaaaga uuucaaguuu ggcaauuacc     3240

gcuccuucau caaccuuacg ccccaggagc agaaggcuuu ucggcaugcc cuguucuugg     3300

cagacgaaaa uccaauaaaa caagcgguga ucagagccau uaacaauagg aaccgaacau     3360

uuguuaaugg uacccagcgc uacuucgcug aggugcucgc caacaauauc uaucugcggg     3420

cuaaaaagga gaaccuaaac acugacaaga uuaguuuuga uuacuucggg auccccacca     3480

uaggcaaugg aagaggcauu gcugaaauca ggcagcugua ugagaaagua gacagcgaua     3540

uccaggccua cgcaaagggg gacaaaccuc aagccucuua uucccaccuu auugaugcua     3600

ugcucgcguu cugcauagcc gcagacgaac aucguaacga uggaucaauc ggucuggaga     3660

uugacaagaa uuacaguuug uauccacugg auaaaaacac aggcgaaguc uuuaccaagg     3720

acaucuucag ccagauuaag aucacugaua augaguuuuc ggacaaaaag uuagugcgca     3780

aaaaggcuau agagggguuc aacacacacc ggcagaugac gagagaugga aucuacgccg     3840

aaaauuaccu cccgauucug auccacaaag agcuuaacga aguuaggaag ggcuauaccu     3900

ggaaaaauuc cgaggaaauu aagaucuuua aggguaaaaa guacgacaua cagcaacuga     3960

acaauuuggu guauugucuc aaauucgucg acaagcccau uucuaucgau auccagauua     4020

gcacucugga ggagcuacga aacauccuga caaccaauaa cauagccgcu acagcagaau     4080

acuauuacau uaaucuuaaa acucagaagc uccaugagua uuacaucgaa aacuacaaca     4140

ccgcccuggg guauaagaaa uacucaaagg agauggaguu uuugcggagu uuagcguauc     4200

gcuccgaaag agugaaaauu aagucuaucg acgauguaaa acaagugcug gacaaggaua     4260

gcaauuucau aauuggcaag aucacgcucc cuuuuaaaaa ggaguggcag aggcuguacc     4320

gggaauggca gaacaccaca aucaaagacg auuaugaguu ccuuaagucc uuuuucaaug     4380

uuaaaucaau uacuaagcug cacaagaaag uccgaaagga cuuuagcuug ccaaucagua     4440

ccaacgaagg aaaauuccua gugaagagaa aaacauggga uaauaacuuc auuuaccaga     4500

uacugaauga cucugauucc cgcgcugacg gcacuaagcc cuuuaucccu gccuucgaca     4560

uuagcaaaaa cgagaucgug gaggcaauca uugauucguu uaccucaaag aauauauucu     4620

ggcucccaaa gaacaucgaa cugcaaaaag ucgacaauaa gaacauuuuu gccaucgaua     4680

cgucuaaaug guucgagguu gaaacaccca gugaccuuag ggauauuggg aucgcuacca     4740

uacaguauaa gaucgacaau aacucccguc cgaaagugcg ggugaaguua gauuacguaa     4800

uugacgauga cagcaagauc aauuacuuua ugaaccauuc ucugcucaaa agcagauauc     4860

cugauaaggu cuuggagauu cugaaacagu ccacuaucau agaguucgaa ucaaguggau     4920

uuaacaagac aauuaaagag augcucggca ugaagcuggc agguaucuac aaugaaacca     4980

gcaacaauaa gaggccagcc gcgacuaaaa aggcugggca ggccaaaaag aaaaagggac     5040

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     5100

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     5160

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     5220

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     5340

ggcucuuuuc agagccacca gaauu                                           5365


<210>  3588
<211>  3637
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3588
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaagagg aacuacauuc      120

ugggacucga cauagguauc acaucaguug gcuaugggau uaucgauuac gagacacggg      180

acgugauuga ugccggagua cgccuuuuca aagaagcgaa uguggagaac aaugaaggcc      240

gacguuccaa gagaggugcu aggcggcuga agcgcagaag gcggcaucgc auccagcgag      300

ucaaaaaguu guuauuugac uauaaccugc uaacugauca cucugagcuc ucagggauaa      360

auccauacga agccagagug aaaggacuga gucaaaaacu uagcgaggag gaguucuccg      420

cagcccuguu gcaucucgcu aaaaggcggg gcguucacaa cgugaaugaa gucgaagagg      480

acacgggcaa cgagcugucu accaaggaac agaucucgcg uaauucaaag gcacuggagg      540

aaaaauaugu ggccgagcuc caguuagaac gccugaagaa agauggggag guaagaggaa      600

gcauuaacag guuuaagaca agugacuacg uuaaagaggc uaagcagcuu uugaaagugc      660

aaaaggccua ucaucagcug gaucaguccu ucaucgacac uuacauugau cuacucgaaa      720

cccggcgaac auacuaugag gguccgggcg aagggucucc cuuuggaugg aaggacauca      780

aagaguggua cgaaaugcug augggccacu gcaccuauuu cccugaggag cuucgcagcg      840

ucaaguacgc guauaaugca gaucuguaca acgccuugaa ugaccucaac aaucugguga      900

uaacuagaga cgaaaacgag aaauuagaau auuacgagaa guuucagauu aucgaaaacg      960

uguucaaaca aaagaagaaa ccaacacuga agcagauugc uaaagagauc cucgucaaug     1020

aggaagauau caaggguuac aggguuaccu ccacggggaa acccgaguuu acuaaccuua     1080

agguguauca cgacauuaag gauauaaccg cccggaaaga aaucauugag aaugcagagc     1140

uguuggacca gaucgcuaag auacuaacaa uuuaccaguc aagugaagau auccaagagg     1200

aacugacuaa ccucaauagc gagcugaccc aggaagagau cgagcagauu ucuaaccuua     1260

aaggauauac aggcacccau aaucuguccu ugaaggccau caaccucauu cuggacgaau     1320

uauggcacac gaaugauaac caaauagcga ucuucaaucg ccugaaacuu guaccuaaga     1380

agguggaccu cucacagcag aaagagauuc caacuacacu ggucgaugac uuuauccuua     1440

gccccguggu uaagagaagu uucauccagu cuauuaaagu gauaaacgcc aucauuaaga     1500

aauacggcuu gccuaaugau aucauuaucg aacuggcaag ggagaagaac uccaaagacg     1560

cucaaaagau gauaaaugaa augcagaagc guaaccggca gaccaacgag cgaaucgagg     1620

aaauuaucag aacuacaggg aaagagaaug ccaaguaucu cauugaaaag aucaagcugc     1680

augacaugca ggagggaaaa ugucuuuaca gccuggaagc uauaccauug gaggaucucc     1740

ugaacaaucc guuuaacuau gaggucgacc acauuauccc caggucggug ucuuucgaua     1800

auuccuucaa caauaaggua uuaguuaagc aagaagagaa cucaaagaag gguaaucgca     1860

ccccuuuuca guaccugagu agcuccgacu cuaaaauuag cuacgaaacg uucaagaaac     1920

acauccuaaa ccucgccaag ggcaaggggc ggauaucaaa aaccaagaaa gaguaucugc     1980

uugaggaaag agauaucaau agguuuagug ugcagaagga cuucauuaac cgcaauuugg     2040

ucgauacacg auacgcaacu cggggacuga ugaaccuccu gcguuccuau uuuagaguga     2100

auaacuuaga cgucaaagug aaaagcauca acggcgguuu caccucuuuu cugaggcgca     2160

aguggaaauu caagaaagag cggaauaagg gguacaaaca ucacgccgaa gaugcucuua     2220

uuaucgcgaa cgcagacuuu auauucaagg aguggaaaaa guuggauaag gccaaaaagg     2280

uuauggaaaa ucagauguuu gaggagaaac aagcugaauc aaugccagag auugaaacag     2340

agcaggaaua uaaggagauc uucaucacuc cccaucagau uaaacacauc aaggacuuua     2400

aggauuacaa auauagucau agaguggaca agaaaccuaa cagggagcuc auuaaugaca     2460

cccuguacag cacacgaaag gaugacaaag gcaacacccu aauaguaaau aaccugaaug     2520

gacucuacga uaaggacaac gauaagcuga aaaagcuaau caauaaaucc ccagaaaagu     2580

ugcugaugua ucaccacgac ccccagacuu accaaaaauu aaagcucauu auggagcagu     2640

auggcgauga aaagaacccg cuguacaaau auuacgagga aacggggaau uaucuuacaa     2700

aguacucuaa aaaggacaac ggaccuguca ucaaaaagau uaaguacuau gguaauaaac     2760

ugaacgccca uuuggauauc accgacgauu accccaacuc gcgcaauaag gugguuaaac     2820

ucagccugaa gccauaucgg uucgacgugu accuagauaa cggcgucuau aaauucguga     2880

cuguaaagaa ucuggacgug aucaaaaagg agaacuacua ugagguuaau uccaagugcu     2940

acgaagaggc caaaaagcuc aaaaagauau caaaccaggc agaauuuauu gcuaguuucu     3000

acaauaacga ccugaucaaa auuaaugggg agcuuuauag agucaucgga gugaacaaug     3060

auuugcugaa caggauagag gugaauauga uugacaucac cuaccgugaa uauuuagaga     3120

acaugaauga uaagcgcccu ccccggauca uuaagacaau agccucuaaa acccaaagca     3180

ucaagaaaua cuccacugac auucucggca accuguauga agucaagagc aaaaagcacc     3240

cacagaucau uaagaaaggu aagagacccg cagcgaccaa aaaggcuggg caggccaaaa     3300

agaagaaagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     3360

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     3420

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     3480

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     3600

ccccccccca aaggcucuuu ucagagccac cagaauu                              3637


<210>  3589
<211>  4705
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3589
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcacuguuc aacaagugca      120

uuauaaucag cauuaaucuc gacuuuucca acaaagagaa guguaugacc aagccauacu      180

cuaucggacu ugauauuggu acaaauucag uuggcugggc cgugaucacu gacaacuaua      240

aaguaccgag uaagaaaaug aaggugcugg ggaauacgag caaaaaguac auaaagaaaa      300

acuuguuagg aguccugcua uucgauuccg gcaucaccgc ggaagguagg cggcucaagc      360

gcacagcucg acguagauau acuaggcggc gcaauaggau ucuguaccuu caggagaucu      420

uuucuaccga aauggccaca cuggacgaug cauucuuuca aagauuggac gauucguucc      480

ucgugcccga cgauaaacgg gacucaaagu auccuauuuu ugggaaccug guugaggaaa      540

aaguguacca ugaugaguuc ccaaccaucu aucaucugcg caaguaccuc gccgacagca      600

cuaaaaaggc ugauuuacga cuggucuacc uugcauuggc ccacaugaua aaguauagag      660

gacacuuucu gauugagggc gaguucaaua guaaaaacaa ugacauccag aagaacuuuc      720

aggacuuccu agauacauac aaugcuauuu uugaguccga ccucucucug gaaaacagca      780

aacagcuuga ggagaucgug aaggauaaaa ucuccaagcu ggaaaagaaa gacaggauuu      840

ugaaacucuu ccccggcgag aaaaauucag ggauauucag ugaauuucug aaguuaaucg      900

uaggaaacca agccgauuuc cggaaaugcu uuaaccugga cgagaaggcg agccuccauu      960

ucucuaagga auccuaugau gaggaccuug agacccuguu ggguuacauu ggcgaugacu     1020

auucagaugu uuuucuaaaa gcaaagaaac uguacgacgc cauccuccug agcggguucc     1080

uuacggugac ugacaaugaa accgaggcuc cucugaguuc ugccaugaua aagcguuaua     1140

acgaacacaa agaggauuug gcacuccuga aggaauacau ucgcaauauc uccuuaaaga     1200

cauacaacga ggucuuuaaa gacgauacua agaauggaua ugcuggcuac aucgacggua     1260

aaaccaacca ggaggauuuc uauguguacc ugaagaaucu acucgccgaa uuugaggggg     1320

cggacuauuu ccuggaaaag auugauagag aggacuuucu uaggaagcag cggacauucg     1380

auaacggaag caucccauac cagauucacu ugcaagaaau gcgagccaua cuggacaaac     1440

aggcaaaguu uuaucccuuc cucgcuaaga auaaagagcg caucgagaag auucugaccu     1500

ucagaauccc uuacuacgug ggcccacuug ccaggggcaa cucggauuuu gcuuggucua     1560

uccggaaacg caaugaaaag auuacgccgu ggaacuucga ggacgucaua gauaaagaau     1620

ccucagccga ggcauuuauc aauagaauga caaguuucga ccuguauuug cccgaggaaa     1680

agguucuccc uaagcauagc cuguuauacg agacuuuuaa cguguauaau gaacugacca     1740

aaguaagguu cauugccgag uccaugcgug acuaccaguu ucuagauucu aagcagaaaa     1800

aggacaucgu gcggcucuau uucaaagaua agcgaaaggu cacugacaaa gauauuaucg     1860

aauaccugca cgcuauauau ggguacgacg gaaucgagcu uaaggguauu gagaaacaau     1920

uuaacagcuc auugaguaca uaccaugauc ugcucaacau cauuaaugac aaggaguucc     1980

uggaugacuc cagcaacgaa gcgaucauag aagagauuau ccacaccuua acgauuuuug     2040

aagauagaga gaugaucaaa cagaggcugu cuaaguucga gaauauauuu gacaagucag     2100

ugcuuaaaaa guugagucgc cggcacuaua ccggcugggg gaaacucagc gcaaagcuga     2160

ucaacggaau uagagaugaa aaauccggca auacaauccu agacuaccug auugacgaug     2220

guaucucuaa caggaauuuc augcagcuca uacaugacga ugcccugucg uucaagaaaa     2280

agauucaaaa ggcucagauc aucggggacg aggauaaagg caacauuaag gaaguuguga     2340

aaagccuucc aggauccccc gccaucaaga aaggcauuuu gcagucaaua aagaucgucg     2400

acgagcuggu gaagguaaug ggagggcgca aaccugaaag uauuguugug gagauggcac     2460

gagagaauca guauacuaac caagguaagu cuaauagcca gcagcgguua aaacgucucg     2520

aaaagucccu gaaagagcuu ggcucaaaga uccugaagga aaacauucca gccaaauuga     2580

gcaagaucga uaauaacgcu cuccagaaug acagacugua ccuauauuac cugcaaaacg     2640

ggaaagauau guauaccgga gacgaucucg acauagacag gcugucuaac uacgauaucg     2700

accacauuau cccccaggcc uuucuuaagg auaauuccau ugacaacaaa gucuugguga     2760

guagcgcauc uaaucgcggc aaguccgaug acuucccguc acuggagguc gugaagaaac     2820

ggaagacauu uugguaccag uuacucaaaa guaagcugau cagccagaga aaauucgaua     2880

accuuacuaa ggcggagagg ggugggcugc uaccugaaga caaggcuggc uuuauacaac     2940

gacagcucgu ugagacccgc cagauuacaa aacauguggc ccggcuguug gaugaaaagu     3000

ucaauaacaa aaaggacgag aauaacagag caguaaggac cgucaaaauc aucacucuga     3060

agucgacguu agugucccag uuucguaaag auuucgaacu cuauaagguu cgcgagauua     3120

augacuuuca ccaugcccac gaugcuuacc ugaacgccgu gauagcaucu gcccuucuga     3180

agaaauaucc caaguuggag ccagaguucg ucuacggaga cuauccuaaa uacaauagcu     3240

uucgggaaag aaagucagcu acagaaaaag uguauuucua caguaacauc augaauauuu     3300

ucaagaaguc caucucucuc gcggacggca ggguaauuga gcgaccccug aucgaaguga     3360

acgaggagac cggggaaagc guuuggaaua aagaguccga ucuagccacu guccggcgcg     3420

ugcugucaua cccacaagug aacgucguua agaaagugga agaacagaac cacggacuug     3480

acagagguaa gcccaaaggc cucuuuaaug cuaaccugag caguaagccu aagccaaauu     3540

cuaacgaaaa uuuggugggg gcaaaagagu aucuggaucc gaagaaauac ggaggcuaug     3600

ccgguauauc caacagcuuc gcuguauuag ucaaggggac caucgagaaa ggcgccaaga     3660

agaaaauuac aaaugugcuc gaauuucagg gaaucucgau ucuggacagg aucaacuacc     3720

ggaaggauaa acuuaauuuc cuguuggaga agggcuauaa agacauagaa cucauuaucg     3780

agcugcccaa guacucacua uuugagcugu cugaugggag ucgaagaaug cuugcaucca     3840

uccucagcac caacaauaaa cgcggagaaa uucauaaggg caaccaaauc uuccugucuc     3900

agaaguuugu uaaauuguua uaucacgcga agaggauaag caauacuauu aacgagaauc     3960

aucguaaaua cguggaaaac cacaagaaag aguucgagga acuguuuuac uauauccucg     4020

aguucaauga aaacuacguc ggugccaaga agaacgggaa acugcuuaau uccgcuuuuc     4080

agucauggca gaaccacagu auugacgagc uguguagcuc uuucaucgga ccuacaggcu     4140

ccgaacggaa ggguuuguuu gagcuaacgu caagaggcag ugccgcagau uucgaguucc     4200

ugggggugaa aauaccaagg uaucgcgacu acacccccag cucccuccug aaggaugcua     4260

cucuuauuca ucaaucuguu acaggauuau augaaacccg aaucgaccug gccaaacucg     4320

gcgaggggaa gagaccugca gccacaaaga aagcuggaca ggcgaagaaa aagaaaggac     4380

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     4440

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     4500

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     4560

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     4680

ggcucuuuuc agagccacca gaauu                                           4705


<210>  3590
<211>  3781
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3590
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaugguac gccagccuga      120

uguccgcgca ucaccucagg guuggaauug acguggguac ccauucugua ggccuugcua      180

cacugcgggu ggaugaccac gggacuccaa uagaguuguu aucagcccug agucauaucc      240

acgauagcgg agucggcaag gaagguaaaa aggaccacga uacgcgcaag aaacuauccg      300

ggauugcacg acgugccaga aggcuccugc aucaccggcg cacccagcuu caacagcugg      360

acgagguguu gagagaucuc ggauucccga uccccacacc uggcgaauuu cuggaccuga      420

acgagcagac ugauccauau aggguuuggc gggugcgcgc ucgacucguc gaagagaagu      480

uacccgagga acugagaggc ccugcaauuu cuauggccgu gaggcauauc gcucggcacc      540

guggguggcg caauccauac ucgaaaguag agucacuuuu gagccccgcc gaagagaguc      600

cuuucaugaa ggcgcugaga gagaggauac uagcaaccac aggagaaguu cucgacgaug      660

guaucacccc aggccaggcc auggcucaag uggcccugac ucacaacauu uccaugcggg      720

ggccggaggg aauccuuggc aaacugcauc agucugacaa ugcaaacgaa auucgaaaga      780

ucugcgcucg ccaggguguc agccccgaug uguguaagca guugcucaga gccguguuua      840

aagcggacuc cccuaggggg ucagccguca gucggguugc accagacccc cugccuggac      900

aaggcagcuu ccgcagagcu ccaaagugcg aucccgaguu ucagagguuc cguauaauuu      960

cuaucguggc caauuuacgg auuuccgaaa caaaaggcga gaaccgaccg cugaccgcug     1020

acgagagaag gcacguagug acguuucuca cugaagauuc acaggccgac cuuaccuggg     1080

ucgauguggc agagaagcug gggguucauc gccgggacuu gagaggaaca gccgugcaca     1140

cugaugacgg ugaaaggagc gcugcgcgcc cucccaucga ugcaaccgac cgaaucaugc     1200

ggcagacaaa aauuaguucu cuaaagaccu ggugggagga agccgauucc gagcaacgug     1260

gcgcuaugau aagauaucug uacgaggacc caacggacag cgaaugugcc gagaucauug     1320

cagaacuccc ugaggaagau caggccaaac uggacucgcu ucaucugccc gcugggaggg     1380

ccgcauauuc ucgcgagucc uugacugcgc ucucagauca caugcuggcu acaaccgacg     1440

auuuacacga ggcccggaag agacuguucg gagucgacga uaguugggca ccacccgccg     1500

aagcuaucaa ugccccugug ggcaacccaa gcguagacag gacucuaaag auaguugguc     1560

gauaccucuc cgcaguggag ucuauguggg ggacaccgga agucauucau guggagcacg     1620

uccgcgaugg cuuuaccagc gagcggaugg cugacgaaag agauaaagcc aauaggcguc     1680

gcuauaacga caaucaggag gcgaugaaga aaauccaacg ggauuacgga aaggaaggcu     1740

acaucucaag aggggacauu gugaggcugg acgcccuuga guugcaggga ugcgcuuguc     1800

uguauugcgg uacgaccauc ggcuaccaua caugucagcu cgaucacauu guuccccagg     1860

cagggccugg aaguaacaau cgacggggca accugguggc cguaugcgaa cgcuguaaua     1920

gauccaaaag caacacucca uucgcugucu gggcccaaaa gugcgguaua ccccacgugg     1980

ggguuaagga ggcaaucggc agggugcggg gauggcgaaa acagaccccu aacacaucuu     2040

cagaggaccu uacuagacug aagaaagaag ucauugcgcg cuugaggcgu acccaggagg     2100

auccagaaau cgacgagcgg aguauggaaa gcguggccug gauggcuaau gagcuccauc     2160

acagaaucgc cgcagcuuau cccgagacaa ccguaauggu guacaggggc uccauuacug     2220

ccgcagcccg caaggcugcg gggauagauu cucgaaucaa ccugauugga gaaaaaggua     2280

gaaaggaccg gaucgauagg cgccaucacg ccguugacgc aucggucgug gcuuuaaugg     2340

aggccagcgu ggccaagacg cuggcugaac guuccucacu aagaggcgag cagcggcuca     2400

cagggaaaga acaaaccugg aagcaguaua cuggaaguac cgucggcgca agggagcauu     2460

uugagaugug gcgcggucac augcugcacc uuacagaauu guucaaugag cgacuggccg     2520

aagauaaagu uuacgugacc cagaacauua gacuccggcu gucugacggg aaugcgcaua     2580

cugugaaccc gagcaaguua guaucccaca ggcugggcga uggacuuaca guccagcaaa     2640

ucgaccgcgc uuguacgccu gccuuguggu gcgcacucac cagagagaaa gauuuugacg     2700

agaagaaugg ccugccagcc agggaagacc gggcuauacg ugugcauggg cacgagauca     2760

agucaagcga uuauauucag guuuucucua aacgcaagaa aacugacucc gaucgagacg     2820

aaacacccuu cggagcaauc gccgugagag gcgguuuugu cgagauuggg ccuaguaucc     2880

aucacgcgag gauauaccgg guggaaggaa agaaacccgu uuacgcuaug cuacgcgugu     2940

ucacccacga ucugcucagc cagagacaug gcgaccuguu uucugccgua auuccaccuc     3000

aguccaucuc aaugaggugc gcagagccca aacuucggaa agccauuaca acugguaacg     3060

cuaccuauuu gggcuggguc gugguggggg augagcugga aaucaauguc gacaguuuca     3120

cgaaguacgc cauaggacga uuuuuagagg auuucccaaa caccacaaga uggcgcaucu     3180

guggcuauga cacuaauagc aagcucaccc ugaaaccgau uguucuugca gcugaagggc     3240

uggagaaccc uucguccgcc gugaaugaaa ucguagaguu gaagggaugg aggguggcga     3300

uuaacguccu cacaaaagug caccccacug uugugcggcg ugaugcccug ggcagaccaa     3360

gguacucuag ccgcucaaau cuacccacca guuggacgau cgagaagcgg ccugcagcua     3420

caaagaaggc cggucaagcu aagaaaaaga aaggacuagu gcaucacauu uaaaagcauc     3480

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     3540

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     3600

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     3720

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     3780

u                                                                     3781


<210>  3591
<211>  4642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3591
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaccaag ccauacagca      120

uuggacugga cauagguaca aacuccguug gcugggccgu gacuacggau aauuauaaag      180

uaccgucuaa gaagaugaaa gugcucggga acaccucaaa gaaauacauc aagaaaaauc      240

uucugggagu cuuguuauuc gacaguggca uuacagcgga ggguaggcgg cugaagcgca      300

cugcucgacg uagauauacc aggcggcgca acagaauccu auaccuccag gaaauuuuua      360

gcacagagau ggccacccug gaugacgcau ucuuucaaag gcuugaugac uccuuccugg      420

ugcccgauga caagcgggau ucuaaauauc cuaucuuugg gaauuugguu gaagagaagg      480

ccuaccauga cgaguuccca acuauauauc accuccgcaa auaccuggcu gauucgacaa      540

agaaagcaga ccugcgacuc guguacuuag cccuggcuca uaugaucaag uauagaggac      600

acuuucuuau ugagggcgag uucaacucaa agaauaacga uauccagaag aauuuucagg      660

acuucuugga caccuacaac gccauuuuug aaagcgaucu gagucuagaa aauuccaagc      720

agcucgaaga gaucgucaaa gacaagauau cuaaacugga gaagaaagau aggauucuua      780

agcuguuccc cggcgaaaag aacagcggga ucuuuuccga guucuugaaa cucauugugg      840

gaaaucaagc ggacuuccgg aagugcuuua accuggauga aaaagcauca uuacauuuca      900

guaaggagag cuaugacgaa gaucuggaga cgcuccuugg uuacaucggc gacgauuauu      960

cugacguauu ucugaaagcc aagaaguugu acgaugcuau ccuacugucc ggguuccuca     1020

cuguuaccga caaugagaca gaagccccuc ugucaagcgc aaugauuaaa cguuauaacg     1080

agcacaagga agaccuugcu cuguugaaag aguacauacg caacaucagu cucaagacuu     1140

acaaugaagu guuuaaagau gacaccaaga acggauaugc cggcuacauu gaugguaaga     1200

caaaucagga ggacuucuau gucuaccuga aaaaguuacu ggcggaguuu gaaggggccg     1260

auuauuuccu agagaaaauc gacagagaag auuuucucag gaagcagcgg accuucgaca     1320

acggaucuau accauaccag auucaccuuc aagagaugcg agcaauccuu gauaaacagg     1380

cuaaguuuua ucccuucuug gccaaaaaua aggaacgcau cgagaagauu cugacguuua     1440

gaaucccuua cuacgugggc ccacucgcua ggggcaacuc cgacuucgcc uggagcauuc     1500

ggaaacgcaa ugagaagaua acuccgugga acuucgaaga ugugaucgac aaagagucgu     1560

cugcagaagc cuuuauuaau agaaugacau ccuucgaucu guaucuuccc gaggagaagg     1620

uccugccuaa acauucauug cucuacgaaa ccuuuaacgu uuauaaugag cugacuaagg     1680

ugagguucau cgcugaaagu augcgugacu accaguuuuu agacagcaag cagaaaaagg     1740

auaucguacg gcuguauuuc aaagacaagc gaaaagugac agauaaggac auuauagagu     1800

accuacacgc gaucuauggg uacgauggaa uugaacucaa ggguaucgag aaacaauuua     1860

acuccucucu gagcaccuac caugaccuuu ugaauauuau caacgauaag gaguuccugg     1920

acgauucaag uaaugaagca auaaucgagg aaauuaucca cacgcucacc auuuuugagg     1980

acagagaaau gaucaaacag aggcugucca aguucgagaa cauauuugau aaaagcgucu     2040

uaaagaagcu gucucgccgg cacuauacag gcugggggaa acuuucagcc aaguugauua     2100

acggaaucag agacgagaaa aguggcaaua cuauucucga uuaccugauc gacgacggua     2160

uaagcaacag gaauuucaug cagcuaaucc augaugacgc ucuguccuuu aagaaaaaga     2220

uucaaaaagc ccagaucauu ggggaugaag acaagggcaa caucaaggag gugguuaaau     2280

cucucccagg aucgcccgca auaaagaaag gcauucugca gagcaucaag aucguggaug     2340

aacuugucaa agugauggga gggcgcaagc cugaguccau uguaguugaa auggcccgag     2400

agaaucagua uaccaaccaa gguaagucaa auagucagca gcgguugaaa cgucuggaga     2460

agucuuuaaa agaacucggc agcaagaucc ugaaagagaa cauuccagcu aagcugucca     2520

agauagacaa uaacgcccug cagaaugaua gauuguaccu cuauuaccug caaaacggga     2580

aagacaugua uacaggagau gaccuagaua ucgacaggcu gucaaauuac gacauugauc     2640

acaucauucc ccaggcauuc cucaaggaca acagcaucga uaauaaagug cuggucucuu     2700

ccgcgaguaa ccgcggcaag agcgacgaug ugccgucucu ugaagucgug aaaaagcgga     2760

agacuuucug guaccaguug cugaaaucca aguuaauauc acagagaaaa uuugacaacc     2820

ucaccaaggc ugagaggggu gggcugaguc cugaggauaa agccggcuuc auccaacgac     2880

agcuuguuga aacacgccag auuaccaagc auguggcacg gcugcuagac gagaaguuua     2940

auaacaaaaa ggaugaaaau aacagagccg uaaggacugu caaaaucauu acgcucaaga     3000

gcacacuggu gucgcaguuc cguaaagacu uugaguugua uaagguucgc gaaaucaaug     3060

auuuccacca ugcucacgac gccuaccuga acgcaguggu cgcuuccgcc uuacucaaaa     3120

aguaucccaa gcuggagcca gaguuugugu acggagauua uccuaaauac aauucuuucc     3180

gggaaagaaa gagcgcgacc gagaaaguau auuuuuacuc aaacauaaug aauauuuuca     3240

agaaaaguau cucccuugcc gacggcaggg ugaucgaacg accccugauu gagguuaacg     3300

aagagacugg ggagucuguc uggaauaagg aaagcgacuu ggcuaccgug cggcgcgugc     3360

ucuccuaccc acaagucaac guugugaaga aaguggagga acagaaucac ggacuggaua     3420

gagguaagcc caaaggccua uuuaacgcaa aucugucaag caagccuaaa ccaaacagua     3480

acgagaaucu uguaggggcc aaggaauauc ucgacccgaa gaaauacgga ggcuaugcug     3540

guauaucuaa cuccuucaca guccugguga aggggaccau cgagaaaggc gccaagaaaa     3600

agauuacuaa uguuuuggag uuccagggaa ucagcauucu ggauaggauc aacuaccgga     3660

aggacaaauu aaauuuucuc cuggaaaagg gcuauaaaga uauagagcuu aucauugaac     3720

ugcccaagua cucguuguuc gagcucucag acgggucucg aagaaugcug gcaaguaucc     3780

uauccacaaa caauaaacgc ggagagauuc auaagggcaa ccaaaucuuu cugagccaga     3840

aauucgugaa acuucucuau cacgcgaaga ggauaucuaa uacgauuaac gaaaaucauc     3900

guaaauacgu cgagaaccac aagaaagaau uugaggaacu guucuacuau aucuuggagu     3960

uuaaugagaa cuacgugggu gccaagaaaa augggaaguu acugaacagc gcuuuccagu     4020

ccuggcagaa ucacucaauc gaugaacucu guaguagcuu uauuggaccu accggcucug     4080

agcggaaggg ucuguucgaa cuuacuucca gaggcucagc cgcagacuuu gaguuccugg     4140

ggguuaaaau cccaagguau cgcgauuaca cacccaguag cuugcuaaag gacgcuaccc     4200

ugauacauca auccgugaca ggacucuaug aaacucgaau ugaucuggcc aaacuuggcg     4260

aggggaagag accugcagcc accaaaaagg cuggacaggc gaagaaaaag aaaggacuag     4320

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     4380

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     4440

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     4500

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     4620

ucuuuucaga gccaccagaa uu                                              4642


<210>  3592
<211>  3724
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3592
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcagccgcg uucaagccaa      120

acagcauuaa uuacauacug ggacucgaca ucgguauugc uuccguuggc ugggccaugg      180

uggagaucga ugaagaggaa aacccgauua ggcuuaucga ccugggggua cggguguuug      240

agcgcgcaga aguccccaaa accggagauu cuuuggccau ggcucgacgu uuagcaagau      300

cagugaggcg gcugacacgc agaagggccc aucggcuacu ccgcacucga agacugcuua      360

agagggaggg cguucugcag gcugccaauu ucgacgagaa cgguuugaua aagagucucc      420

cuaauacgcc auggcaacug cgggcggcag cccuggaucg uaaacucacc cccuuagaau      480

ggagcgcugu gcugcuucac uugaucaagc aucgcgggua ucugucccag aggaaaaacg      540

agggagaaac agccgacaag gagcuaggcg cacuccugaa aggcgucgcu gggaaugccc      600

acgcgcuuca gacuggagau uuuaggaccc cugccgagcu ggcauugaac aaguucgaaa      660

aggagucugg ucauauucgg aaucagcgau cggacuacuc acacacauuu agccgcaaag      720

aucuccaagc ugaacugauc uuacuguucg agaagcagaa agaauuuggc aacccacacg      780

ugaguggggg acucaaggag ggcauugaga cccuucugau gacucagaga cccgccuugu      840

ccggugacgc uguacagaaa augcuagggc auugcacauu cgaaccugcc gagccaaagg      900

cagccaaaaa uaccuauacg gcugaaaggu uuaucuggcu gacuaagcuc aacaaucugc      960

ggauacuuga gcaaggaucu gaacgcccgc ugaccgauac agagagagcg acuuugaugg     1020

acgagcccua cagaaagagc aaacucaccu augcacaggc ccguaagcug uuaggccugg     1080

aagauacagc uuucuuuaaa ggccuacggu acgggaagga caacgccgag gcauccaccc     1140

ucauggaaau gaaagccuau cacgcuauuu cacgagcccu ggagaaggaa ggacuuaagg     1200

acaaaaagag uccuuugaau cugagcccag agcuccagga ugagaucggu acggcauucu     1260

cucuguuuaa aacugacgaa gauauuacag gcagacuuaa ggacaggauc cagcccgaga     1320

uccuggaagc guugcucaaa cauauuuccu ucgauaaguu cguucaaaua ucacugaagg     1380

cuuuacgccg gaucgugccu cugauggagc aggggaaaag auacgacgag gccugugcag     1440

aaauuuacgg agaucacuau ggcaagaaaa acaccgagga aaagaucuac cuaccaccca     1500

uaccggccga cgagauuagg aacccugucg ugcuccgcgc ucugagccag gcccgaaaag     1560

ugaucaaugg ugucguucgg cguuauggga gucccgcaag aauccacauu gaaacugcua     1620

gggagguggg caagucuuuu aaggaucgca aagagaucga aaagcggcaa gaggaaaaca     1680

gaaaagacag ggagaaggcc gcggccaaau uccgagaaua cuuuccaaau uucguaggag     1740

agccuaaguc caaagauauu cuuaaguugc gccuguauga gcagcagcau ggcaagugcc     1800

ucuacagcgg gaaagaaaua aaccugggac gguuaaauga gaaggguuau guggaaaucg     1860

accacgcucu gcccuuuucg agaacauggg acgauucuuu caacaauaaa guccuugugu     1920

ugggcuccga gaaccagaau aaggggaacc aaaccccaua cgaauacuuu aauggaaaag     1980

acaacucaag ggaguggcag gaguucaagg cacguguuga aacgagucgc uuuccccgga     2040

gcaagaaaca gaggauucuc cugcagaagu ucgaugagga cggcuuuaaa gaaagaaauc     2100

uaaacgauac ccgauaugug aaucgguucc ugugucaauu ugucgccgac cgcaugagac     2160

ucacagguaa ggggaaaaag aggguguucg cuuccaacgg ccagaucacu aaccugcuuc     2220

ggggauucug gggcuugcga aagguaagag ccgagaauga ucgccaucac gcacuggacg     2280

cgguuguggu cgccugcucu accguggcua ugcagcagaa aaucacaagg uuuguccguu     2340

acaaggagau gaacgccuuc gaugggaaaa cuauugacaa ggaaaccgga gagguguuac     2400

aucagaaaac acacuuuccu cagccauggg aguucuuugc acaggaaguu augauacggg     2460

uguucgguaa gccggauggc aagcccgaau uugaggaggc ugacacccuc gaaaaacuga     2520

gaacucuucu ggccgagaag uugagcucaa ggccugaagc aguacacgag uaugucacgc     2580

cacucuucgu gagucgcgcc cccaaucgaa aaauguccgg gcagggacau auggaaacag     2640

uuaagagcgc uaaaagacug gaugagggcg ugucuguccu acgggugccu cugacccaac     2700

ucaagcugaa agaccuugag aagaugguaa acagggaacg cgagccaaag uuguacgaag     2760

cgcugaaagc ccguuuagag gcacacaagg acgaucccgc uaaagccuuu gccgaaccgu     2820

ucuauaagua cgacaaagcu gguaauagaa cucaacaggu gaaggcaguu cgggucgagc     2880

aagugcagaa gaccggggug ugggucagga accauaaugg caucgccgau aacgcgacaa     2940

ugguucgcgu ggacguguuu gagaaaggag auaaguauua ccucguaccu auuuacucau     3000

ggcaggucgc uaaaggcauc cugccagacc gagccguggu ucaggggaag gaugaagagg     3060

acuggcaacu uauugaugac aguuucaauu uuaaauucag ccugcacccc aacgaucuag     3120

uggaagucau caccaagaag gcaagaaugu ucggauauuu ugccuccugu caccggggca     3180

cugguaauau aaacaucagg auucaugacc ucgaucacaa aaucgggaag aauggcauuc     3240

uggagggaau cggugugaaa acagcuuugu cuuuccagaa guaccagaua gacgagcugg     3300

gcaaagaaau ucgcccuugc agauuaaaga agaggccccc aguucggaaa cguccugcag     3360

ccacgaagaa agcggggcag gcuaagaaaa agaagggacu agugcaucac auuuaaaagc     3420

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     3480

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     3540

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     3720

aauu                                                                  3724


<210>  3593
<211>  4480
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3593
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaagaaa ccauacacca      120

uuggacugga cauagguaca aacagcguug gcugggccgu gcucacugau caguaugacc      180

uuguaaagag gaagaugaaa aucgcggggg auuccgagaa gaaacaaauu aagaaaaauu      240

ucuggggagu gcggcuguuu gacgaaggcc agacggcugc cgaucgccga auggcacgua      300

ccgccagaag gcggaucgag cgcagaagga accggauuuc uuacuugcag gguaucuucg      360

cugaagagau gucaaagaca gacgcaaacu uuuucugccg cuuaagugau agcuuuuaug      420

ucgacaauga aaagcgaaau uccagacauc cguucuuugc cacuauagag gaggaagugg      480

aguaccacaa aaacuauccc accaucuacc aucugaggga ggagcuaguu aauucuucgg      540

aaaaggcuga ucuccggcug guguaucuug cccuggcgca cauuaucaaa uaccguggga      600

acuucuugau ugaaggagca cucgacacac agaauaccuc agucgauggc aucuacaagc      660

aauuuauaca gacuuauaac cagguguucg ccagcggcau ugaggacggg agucugaaaa      720

agcuggaaga uaauaaagac guagcuaaga uccucguuga gaaggugaca cgcaaagaaa      780

aguuagagag gauucugaaa cuuuacccug gagagaaguc cgccgguaug uuugcacagu      840

ucaucucuuu gaucgucggc agcaaaggga acuuucaaaa gccauucgac cugauugaaa      900

aguccgauau agagugugcu aaagacucau augaagagga ucuagaaagu cuccuggccc      960

uuaucggaga cgaguacgcg gagcuguucg uggccgcaaa gaaugcuuau agcgccgugg     1020

ucuugucuuc cauuaucacc guugcugaaa cggagacuaa cgccaaacuc ucagcaagca     1080

ugauagaaag guuugauacc caugaggaag accugggcga guuaaaggcc uucauuaaac     1140

ugcaccuccc caagcacuac gaggaaaucu uuaguaauac agagaagcau gguuaugcug     1200

gguacaucga uggaaaaacu aagcaggcgg acuucuacaa auauaugaag augacccuug     1260

aaaacauuga gggcgcagau uacuuuaucg ccaaaauuga gaaggaaaac uuccugcgga     1320

agcagcgaac auuugacaau ggcgcuauac cucaccaguu gcaucuagag gaacuggagg     1380

ccauccucca ccaacaggca aaauauuacc cauuccugaa ggaaaacuau gauaaaauua     1440

agucucuugu gaccuuucgc auccccuacu ucguagggcc ucuggccaau ggacaguccg     1500

aguuugcuug guugacgaga aaagccgacg gugagaucag gccauggaac auugaagaga     1560

agguggacuu cggcaaaagc gcagucgauu uuauagaaaa gaugacuaau aaggacacau     1620

aucucccgaa agagaacgug cugcccaagc acucguuaug cuaccaaaaa uaccugguuu     1680

auaaugaacu aaccaaggug cgguacauca acgaucaggg gaaaacuucu uauuucuccg     1740

gacaggagaa ggagcagauu uucaaugacc ucuuuaagca aaaacgcaag gucaaaaaga     1800

aagaucugga acuuuucuug agaaacaugu cacaugugga gaguccuaca aucgaaggcc     1860

uggaggacag cuuuaauucc ucuuacagca ccuaucacga ucuccugaag guagguauua     1920

agcaggaaau ccuugacaac ccaguuaaua cggagaugcu ggagaacaua gugaaaaucu     1980

ugaccgucuu cgaagauaag aggaugauua aagagcagcu ccagcaauuu ucagacgugc     2040

uggauggggu cguguuaaag aaacuggaac gucggcauua cacaggcugg ggacgacuaa     2100

gugcgaagcu ccugaugggc aucagagaca agcaguccca ccuuacuauu uuggauuauc     2160

ugaugaauga cgacgggcuc aacaggaacc ugaugcaguu aaucaaugau agcaaccugu     2220

cuuucaaauc aauaauugag aaggagcagg uuaccacagc ugacaaagau auccaaagua     2280

uuguggccga ccuugcagga agccccgcca ucaagaaagg uauauugcag ucccucaaga     2340

ucguagauga acuggucucu gugaugggcu acccuccaca gacuauuguu guggagaugg     2400

cucgcgaaaa ucagaccaca gggaaaggaa agaacaauuc gcggcccaga uacaagagcc     2460

uagagaaagc caucaaggaa uuuggcuccc aaauucugaa agagcauccg accgacaacc     2520

aggagcucag gaauaaccgc cuguaucuuu acuauuugca gaaugguaag gauauguaca     2580

cugggcaaga ccuggauauc cacaacuuau caaauuauga cauagaucac auugucccuc     2640

agaguuucau cacggacaac ucuaucgaua aucucgugcu gacaagcucc gcaggcaacc     2700

gagaaaaagg agacgacgua cccccacuug agauugugcg gaagcguaag gucuuuuggg     2760

aaaaacugua ccagggcaau uugaugucaa agagaaaauu cgauuaucuc accaaggcug     2820

agagaggggg acugacugaa gccgacaaag cgcgcuuuau ccaucggcag cuaguugaga     2880

ccagacaaau uacaaagaac guggccaaca uacugcacca gagguucaau uacgagaagg     2940

augaccaugg uaacaccaug aaacaggugc gaaucgucac ucucaagagc gcucugguuu     3000

cucaguuucg caaacaauuc cagcuuuaca aggugcggga ugugaaugac uaucaccacg     3060

cacaugaugc cuacuugaac ggcguagucg cuaauacacu guuaaaagug uauccucagc     3120

ucgaacccga guucguuuac ggggacuauc accaguuuga uugguucaag gccaacaagg     3180

caacggcgaa aaagcaauuu uacaccaaua uuaugcuguu cuuugcccag aaagacagaa     3240

ucauugauga aaacggagag auccuuuggg acaagaaaua ucuggacacu gugaagaagg     3300

ucauguccua caggcagaug aauauaguga aaaagacaga aauccaaaaa ggcgaguuca     3360

guaaggcuac cauuaaacca aaggguaaca gcucuaaacu aaucccccgu aagacaaauu     3420

gggauccuau gaaguacggg ggccucgacu ccccaaacau ggccuaugca guugugauug     3480

aguacgcuaa aggaaagaau aaacugguau uugaaaagaa aaucauacgc gucacuauua     3540

uggagcggaa ggccuucgaa aaggaugaga aagcauuuuu ggaggaacag ggcuauagac     3600

agccgaaggu gcuggccaaa uuacccaagu acacccucua ugagugugaa gaggggaggc     3660

gacggaugcu ggcuucagcg aacgaagccc agaaaggaaa ucaacaggug cuuccuaacc     3720

aucuggucac guugcuccac caugcagcua acugcgaggu uagugacggc aagagccugg     3780

auuacaucga gucgaaucgc gaaauguucg ccgagcuacu ggcccacgug uccgaauuug     3840

cuaagagaua uacccuugca gaggccaacc ucaauaaaau caaccagcug uucgaacaga     3900

auaaggaggg ugacauuaaa gcgauagcuc aaucuuucgu agauuugaug gccuuuaacg     3960

caaugggggc cccagcuagc uucaaguuuu ucgagacaac uaucgaaagg aaacgguaca     4020

auaaccugaa ggaguuacuc aauucaacca uuaucuacca gaguauuaca ggacuguaug     4080

aaucccgaaa gagacuugac gauaaacgcc ccgcagccac uaagaaagcg ggccaggcua     4140

agaaaaagaa aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     4200

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     4260

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     4320

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     4440

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           4480


<210>  3594
<211>  4513
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3594
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaagaaa ccauacagca      120

uuggacugga cauagguacc aacuccguug gcugggccgu gguaacagau gacuauaagg      180

ugccggcgaa gaaaaugaag guccucggga auacugauaa aucucauauc gagaagaacc      240

uucugggagc uuuguuauuc gacucaggca auacggccga agauaggcgg cugaaacgca      300

ccgcacgacg uagauacaca aggcggcgca accggauucu auaucuccag gagaucuuua      360

gugaagagau ggguaaggug gacgauagcu ucuuucacag gcuggaagac uccuuccuug      420

uuacugagga uaagagaggg gagcgccauc ccauuuuugg aaaucuggaa gaggaaguga      480

aauaccacga gaacuucccu accaucuauc auuugcgaca auaccucgcc gacaauccag      540

agaaggucga ucugagacug guguaucucg cuuuagcaca cauaaucaaa uuuaggggcc      600

acuuccugau ugaaggcaag uuugacacac ggaacaauga uguacagcgu cuuuuccagg      660

aguuuuuggc cguuuacgac aacaccuucg aaaauucuuc gcugcaggag caaaacgugc      720

aggucgaaga gauccuaacu gacaaaauuu caaagagcgc uaaaaaggau cgcgugcuca      780

agcuguuucc caaugagaaa aguaacggga gauucgccga guuccuuaag cugaucgugg      840

gaaaucaggc ggacuuuaaa aagcauuucg aguuggaaga gaaagcaccu cuccaguuuu      900

ccaaggauac auacgaagag gagcuggaag ucuuacuggc ccaaauaggu gacaacuaug      960

cugaacucuu ccuuucugcc aagaaacugu acgauagcau uuugcuaucc ggcauccuga     1020

ccguuacgga cguggggacu aaggcaccac ucucagcuag uaugauucag agguauaacg     1080

aacaccagau ggaucuggcc cagcuuaaac aauuuauccg gcagaagcug agcgacaaau     1140

acaaugaggu auucucugau guguccaagg acggauaugc gggcuacauc gaugguaaga     1200

ccaaccagga agccuuuuau aaauacuuga aggggcuccu gaauaaaauu gagggaucag     1260

gcuacuucuu agacaagaua gagcgagaag acuuucugcg caaacaaaga acauucgaua     1320

acggcagcau cccccaucag auucaccuac aggagaugag ggcaaucaua cggcgccagg     1380

cugaauuuua uccuuuccuc gccgacaauc aagauagaau ugagaagcug cuuacuuuua     1440

ggaucccaua cuaugucggg ccguuggcuc guggaaagag ugacuucgcc uggcugucuc     1500

ggaaauccgc agauaagauc acccccugga acuucgacga gauuguggau aaagaaagcu     1560

cggccgaggc uuuuaucaau cgaaugacaa acuacgaccu cuaucugccu aaucagaagg     1620

uucuuccaaa acacucucug uuguacgaaa aguucaccgu guauaacgag cucacgaaag     1680

ucaaguacaa gacugaacag gguaaaacag cguuuuucga ugcaaauaug aagcaggaga     1740

uuuuugacgg cguguucaaa guauacagaa agguuaccaa agauaagcug auggacuuuu     1800

uagagaagga guucgaugaa uuuaggauag uggaccugac ugggcuagac aaagaaaaca     1860

aggucuucaa ugccuccuau ggaacauacc augaucucug caaaauccug gacaaggauu     1920

uucuugacaa caguaaaaau gagaagauuu uggaagauau cgugcugacc cucacgcugu     1980

ucgaggaccg cgagaugauc cggaagagau uagaaaacua uucagaucug cuuaccaaag     2040

agcaagucaa gaaauuggaa aggcgccacu acacaggcug gggucgacuc agcgcugagc     2100

ugauucaugg gauacggaac aaggaauccc guaaaacuau ccuagacuau cugauugaug     2160

acggcaauuc uaacagaaau uuuaugcagc ucaucaacga ugacgcccug agcuucaagg     2220

aggagauugc aaaggcccag gugaucggag aaaccgauaa ucuuaaccag guugugucag     2280

acauagcugg cagucccgcc aucaaaaagg ggauuuugca aucccugaaa aucguagacg     2340

aguuagucaa gauuauggga caccagccug aaaauaucgu gguugagaug gcaagggaga     2400

accaguucac aaaucagggu cgccggaaca gccaacagag acucaaaggc cugacugauu     2460

cuauaaagga auuuggguca cagauucuua aagagcaucc aguggaaaau agucaacugc     2520

agaacgacag guuguuccuc uacuaucugc agaauggacg agauauguac accggcgagg     2580

aacuagacau cgauuaccug agccaguaug acauugauca caucauaccc caagcguuua     2640

ucaaggacaa cuccauugau aaucgcgucc ucacaucuuc gaaggagaac cgggguaaaa     2700

gcgacgaugu gccguccaag gacguaguga gaaaaaugaa gucauacugg aguaaacugc     2760

uuucugcuaa guugaucacc cagaggaagu ucgacaaccu gacuaaagcc gagcgugggg     2820

gcuuaacgga ugacgauaag gcaggauuua uuaaacgcca gcucguugaa acacggcaga     2880

ucaccaagca cgucgccaga auacuggacg agagguucaa uacugaaacc gaugagaaca     2940

auaaaaagau ucgacaagug aagaucguga cacuuaaaag caaccugguc uccaauuuuc     3000

ggaaggaguu cgaacuauau aaaguucgcg agaucaacga cuaccaucac gcucaugaug     3060

ccuaucucaa ugcagugauu ggcaaggcuc uguugggggu guacccucag cuggaacccg     3120

aguuuguaua uggagacuac ccacacuucc acggucauaa agaaaacaag gccaccgcga     3180

agaaauuuuu cuacucaaau aucaugaacu ucuuuaagaa agaugacguc agaacugaua     3240

agaauggcga gauuauaugg aaaaaggacg aacacaucag caacauuaag aaaguguuau     3300

cuuauccuca gguuaauauc gugaagaaag ucgaggagca gacaggggga uucuccaagg     3360

aaaguauucu ccccaaaggc aacagcgaua agcugauccc aaggaaaacg aagaaguuuu     3420

acugggacac caaaaaguau gguggguucg acucucccau aguggccuac uccauccuug     3480

uuauugcuga uaucgagaaa ggcaagagua aaaagcugaa gacugugaaa gcauugguag     3540

gagucacaau uauggaaaag augaccuuug agcgggaccc uguggccuuc cucgagcgaa     3600

aaggcuauag aaaugugcaa gaagagaaca ucauaaagcu gccaaaauac ucacuauuua     3660

agcuggaaaa cgggcgcaag aggcuucucg cuagcgcccg ugagcugcag aaaggaaaug     3720

aaauugucuu gccgaaccau cugggcacau uacucuauca cgcaaagaau auccauaaag     3780

uugaugagcc caagcaccug gacuacgugg auaaacacaa ggacgaguuc aaggaacuuc     3840

uggauguagu gucgaacuuu uccaaaaagu acacuuuggc ggaggguaau cucgagaaaa     3900

ucaaggaacu guaugcccag aacaaugggg aagaccuaaa agagcuggcu ucuagcuuca     3960

uuaaccuucu caccuuuacg gccauaggag cuccugcaac cuucaaguuu uucgauaaaa     4020

auaucgaccg gaagagauac acaucaacua ccgagauucu gaacgccaca uugauccauc     4080

aaaguauuac uggcuuauau gaaaccagga ucgaucugaa uaagcucggu ggcgacaaac     4140

gcccagcagc cacgaagaaa gcugggcagg cgaagaaaaa gaagggacua gugcaucaca     4200

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     4260

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     4320

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     4380

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     4500

agccaccaga auu                                                        4513


<210>  3595
<211>  3646
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3595
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcacagaca acaaaccuga      120

gcuacauucu cggacuugac cuggguauag ccuccguugg cugggcggug guagagauca      180

augaaaacga ggauccaauu ggguugaucg acgugggagu caggauuuuc gaacgggcug      240

aggugccgaa gacuggcgaa ucuuuagccc ugucacgccg acuagcacgu aguacgagaa      300

ggcucauccg gcgcagagcc cauaggcugc uucuggcuaa acgguuuuug aagcgcgagg      360

guauacucag caccaucgau cuggagaagg ggcugcccaa ucaagcaugg gaacuccgag      420

uugccggauu agagagaagg cuguccgcua uugaaugggg cgccgugcuu uugcaccuga      480

ucaaacaucg gggcuaucua ucuaagcgua aaaacgaguc gcagacaaau aacaaggagc      540

ucggggcgcu gcuuucagga gucgcacaga aucaccagcu guugcaaagc gacgauuacc      600

gcacuccugc cgaacucgcu cugaaaaagu ucgccaagga ggaaggucau auuagaaacc      660

agaggggcgc auauacccac acauuuaauc gguuagaccu gcucgcugag cuuaaccugu      720

uguucgccca gcagcaccaa uuugggaauc cacauugcaa agaacacauc cagcaguaca      780

ugaccgagcu acugaugugg cagaagcccg cgcucagugg agaggccaua cugaaaaugc      840

uuggcaagug uacucaugag aaaaacgagu ucaaggcagc uaaacacaca uauuccgccg      900

aacgauuugu guggcugacc aaguugaaua accuccgcau ucuggaggau ggugcugaaa      960

gagccuuaaa ugaggaggaa aggcaacugc uaaucaacca cccuuacgag aagucuaaac     1020

ucacguaugc acagguacgg aagcugcuug gguugagcga acaggccauu uucaaacauc     1080

ugcgcuacuc caaggagaac gcugaaucag cgacuuuuau ggagcucaaa gcauggcacg     1140

ccaucagaaa ggcucuggag aaucaaggac uuaaggacac cuggcaggau cuggccaaaa     1200

agccagacuu gcucgaugaa aucggcacag cauucagucu guacaaaacu gacgaggaua     1260

uucagcagua uuuaaccaac aagguuccca auagcgugau aaacgcccug cuagucucuc     1320

ucaauuuuga caaauucauc gaacuguccc uuaagucauu gaggaagauu cugccucuca     1380

uggagcaagg caaacguuac gaccaggcuu gccgggagau cuaugggcau cacuacggag     1440

aagccaacca gaagacaagc cagcuguuac cagcaauacc ggcgcaagag auucgaaauc     1500

ccguggugcu gagaacccuu agucaggcua ggaaagucau caacgccauc auucgccagu     1560

augguucucc ugcacggguu cauaucgaaa cgggcagaga guuggggaag uccuuuaaag     1620

aaaggcgcga gauucagaag caacaggagg auaaucgaac uaagcgggaa agcgccgugc     1680

agaaauucaa ggaacucuuc ucggacuuuu cuuccgaacc aaaaucaaag gauauacuga     1740

aauuccgucu auacgagcag caacacggaa agugucugua uaguggcaaa gaaaucaaca     1800

uucacagacu caaugagaag gguuacguag agaucgacca ugcucugccc uuuagcagga     1860

caugggauga cuccuucaac aauaaggugc uugucuuggc cucugaaaac cagaauaaag     1920

ggaaccagac cccuuacgag uggcugcaag gcaagaucaa cagcgaacgc uggaaaaauu     1980

uuguggcauu aguucucgga ucacagugca gugcugccaa gaaacagcgg cugcuuacuc     2040

aggugauuga ugacaacaag uucauagaua gaaaucugaa cgacacaagg uauaucgcgc     2100

gauuuuuguc caauuacauu caagagaacc uccugcuagu cggcaagaau aaaaagaacg     2160

uguucacccc aaaugggcag aucacggccc ugcuccgcag ccggugggga cugauuaaag     2220

cuagagaaaa caauaacagg caccaugcac uugaugccau cguaguugcu ugugccaccc     2280

ccucuaugca gcagaagaua acacguuuua uccgcuucaa agaggugcac ccguauaaga     2340

uugagaaucg guacgaaaug gucgaccaag agucagguga aaucauuagu ccucacuuuc     2400

ccgagccaug ggcauauuuc agacaggagg ugaacaucag ggucuuugac aaucauccug     2460

auacuguguu gaaggaaaug cugcccgacc gaccacaggc gaaccaccag uucguucaac     2520

ccuuauucgu gagccgggcc ccuacccgca aaauguccgg ccaggggcau auggagacaa     2580

uaaagucugc uaaaagacuc gccgaaggaa uuucgguacu gaggauccca cuuacucagc     2640

ugaagccgaa ccuacucgag aauaugguca acaaagaacg ggagcccgca cuguacgcug     2700

gcuugaaggc ccgacuggca gaguuuaauc aagauccugc caaggcuuuc gcgaccccau     2760

uuuauaaaca gggugggcaa caggugaagg ccauuagagu ugaacaggug cagaaaagcg     2820

gcgucuuagu gcgcgagaac aauggaguag cagacaacgc uuccaucgug aggacagaug     2880

uuuucauaaa gaauaacaaa uuuuuccucg uccccaucua caccuggcaa guggccaagg     2940

gcauucugcc uaauaaagcc aucguggcuc acaagaacga agacgagugg gaagagaugg     3000

augagggggc aaaguuuaaa uucucacuuu uuccaaauga ccuggucgaa uugaagacua     3060

aaaaggagua cuucuuugga uauuacauug gucucgaucg ugccacgggc aacaucaguc     3120

ugaaagaaca ugacggggag auaucuaagg gaaaggaugg cguuuaucgg guggguguga     3180

aacuagcgcu gagcuucgaa aaguaccagg uagacgagcu ugggaaaaau agacagauuu     3240

gcaggcccca gcagcgccag ccgguccgaa agagaccugc ugccaccaaa aaggcaggcc     3300

aagccaagaa aaagaaagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     3360

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     3420

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     3480

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     3600

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    3646


<210>  3596
<211>  3724
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3596
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcagccgcg uucaagccaa      120

acccgauuaa uuacauacug ggacucgaca ucgguauugc uagcguuggc ugggccaugg      180

uggagaucga ugaagacgag aaccccauuu gccuuaucga ucugggggua aggguguuug      240

aacgggcaga ggucccuaaa accggagacu ccuuggccau ggcucgccga uuagcacguu      300

cugugagaag gcugacacgg cgcagagccc auaggcuacu ccgggcucgc cgacugcuua      360

agagagaagg cguucugcag gccgcggauu ucgacgagaa ugguuugaua aagucacucc      420

caaacacucc cuggcaacug agggcagccg cucuggaucg gaaacucacg ccuuuagagu      480

ggagugccgu gcugcuucac uugaucaagc aucgugggua ucugagccag cgcaaaaaug      540

aaggagagac cgcagacaag gaacuaggcg cucuccugaa aggcgucgcc gauaacgcgc      600

acgcccuuca gacaggggac uuuagaacuc cagcagagcu ggcuuugaau aaguucgaga      660

aggaauccgg acauauuagg aaccagcggg gugauuacuc ucacaccuuu ucgcgaaaag      720

accuccaagc cgagcugauc uuacuguucg aaaagcagaa agaguuuggc aauccccacg      780

ugucaggggg acucaaggaa ggcauugaga cacuucugau gacccagcgc ccugcuuuga      840

gcggugaugc cguacagaaa augcuagggc auuguacuuu cgagccagca gaaccgaagg      900

ccgcuaaaaa cacauauacc gcggagagau uuaucuggcu gacgaagcuc aauaaccuga      960

ggauacuuga acaaggaagu gagcggcccc ugacugacac cgaacgcgca acauugaugg     1020

acgagccuua cagaaagucc aaacucacuu augcccaggc uaggaagcug uuaggccugg     1080

aggauaccgc cuucuuuaaa ggccuacguu acgggaagga caaugcagaa gccucuacac     1140

ucauggagau gaaggcuuau cacgccauua gccgggcacu ggaaaaggag ggacuuaagg     1200

auaaaaaguc cccauugaac cugucacccg aacuccagga cgagaucggu accgcguuca     1260

gucuguuuaa aacggaugag gacauuacug gccgacuuaa ggauagaauc cagccugaaa     1320

uccuggaggc uuugcucaaa cauauuagcu ucgacaaguu cguucaaaua ucucugaagg     1380

ccuuaaggcg caucgugcca cugauggaac aggggaaacg guacgaugag gcaugcgccg     1440

agauuuacgg agaccacuau ggcaagaaaa auacagaaga gaagaucuac cuacccccga     1500

uaccugcuga ugaaauuaga aaccccgucg ugcucagggc ccugucccag gcacgcaaag     1560

ugaucaacgg ugucguucga cgguaugggu caccagcucg uauccacauu gagaccgcca     1620

gagaaguggg caagagcuuu aaggacagga aagagaucga gaagcgccaa gaagagaauc     1680

ggaaagacag agaaaaggcg gccgcuaaau ucagggagua cuuuccuaac uucguaggag     1740

aacccaagag uaaagauauu cuuaaguugc gacuguauga gcagcagcau ggcaaguguc     1800

ucuacucugg gaaagagaua aaucugggac gcuuaaacga aaaggguuau guggagaucg     1860

accacgcacu gccauuuucc cggacuuggg augacagcuu caauaacaaa guccuugugu     1920

ugggcucgga aaaucagaac aaggggaauc aaacacccua cgaguacuuu aacggaaaag     1980

auaauucuag agaauggcag gaguucaagg ccaggguuga gaccucccgu uuuccucgcu     2040

caaagaaaca gcggauucuc cugcagaagu ucgacgaaga uggcuuuaaa gagagaaacc     2100

uaaaugacac gagguaugug aaccgauucc ugugccaauu ugucgcugau cggaugcgcc     2160

ucaccgguaa ggggaaaaag agaguguucg ccaguaaugg ccagaucaca aaccugcuua     2220

ggggauucug gggcuugcgg aagguacgag cagaaaacga cagacaucac gcgcuggaug     2280

ccguuguggu cgcuuguagc acuguggcca ugcagcagaa aaucacccgc uuugucaggu     2340

acaaggagau gaaugcauuc gacgggaaaa caauugauaa ggagacugga gaaguguuac     2400

aucagaaaac ccacuuucca cagccguggg aguucuuugc ucaggaaguu augauacgug     2460

uguucgguaa gcccgacggc aagccugagu uugaagaggc cgacacacca gagaaacucc     2520

ggacccugcu ugcagaaaag cuguccucua gacccgaggc cguacacgaa uaugucacuc     2580

cuuuguucgu gagcagggcu ccaaaccgca aaaugucagg gcagggacau auggagacgg     2640

uuaagagugc gaaacgacuc gaugaaggcg uguccguccu gagagugccc cuaacacaac     2700

ugaagcucaa agaccuggag aagaugguaa aucgggagag ggaaccgaaa cuuuacgagg     2760

ccuugaaagc acgccuggaa gcucacaagg augacccugc caaagccuuu gcugagccau     2820

ucuauaagua cgauaaagca gguaaccgua cccaacaggu gaaggccguu agagucgaac     2880

aagugcagaa gacuggggug uggguccgga aucauaacgg caucgcggac aaugcuacca     2940

ugguuagggu ggauguguuu gagaaaggag acaaguauua cuuaguaccc auuuacagcu     3000

ggcaggucgc caaaggcauc cucccugauc gcgcaguggu ucaggggaag gacgaggaag     3060

auuggcaacu gauugacgau ucuuucaacu uuaaauucuc acuucacccc aaugaccugg     3120

uggaggucau cacaaagaag gcccgaaugu ucggauauuu ugcuaguugc cacagaggca     3180

ccgguaacau aaauauccgg auucaugacc uagaucacaa aaucgggaag aacggcauuc     3240

ucgaaggaau cggugugaaa acugcacuga gcuuccagaa guaccagaua gacgaguugg     3300

gcaaagagau uaggccaugu cgccugaaga agagaccucc cguuaggaaa cggccagccg     3360

cgacaaagaa agcugggcag gccaagaaaa agaagggacu agugcaucac auuuaaaagc     3420

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     3480

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     3540

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     3720

aauu                                                                  3724


<210>  3597
<211>  3730
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3597
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcac       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaaguac cacguuggaa      120

uugacguggg uaccuucagc guaggccugg ccgcgauaga gguggaugac gcugggaugc      180

caaucaaaac acucucccuu gucucucaca uucaugauuc aggacuggac ccggaugaaa      240

ucaagagugc cgugacuagg uuggcaagcu ccggcauugc ccggcgcacg cgacguuuau      300

auagaaggaa gcggcgcaga cugcagcaac uagacaaauu uauccagagg caggguuggc      360

ccguuauaga gcucgaagau uacucugacc cucuguaucc auggaaggug cgggcugagc      420

uugcagccuc guacaucgcu gaugaaaaag agcgcgggga gaagcuguca gucgccuugc      480

gacacauugc gagacauagg ggauggcgga accccuaugc aaaagugagc agucucuacc      540

ugccugacgg cccauccgau gccuucaagg cuauccguga agagauuaag cgcgccucug      600

gccagcccgu accugaaacc gcaacaguug ggcaaauggu gacucugugc gagcucggaa      660

ccuuaaaacu gagaggugag ggcggggucc uuagcgcuag guugcagcag uccgacuaug      720

cccgggaaau ccaggagaua ugucgaaugc aagaaauugg acaggagcug uaccgcaaga      780

ucauugaugu gguguuugcg gccgaaucac caaaaggcag ugcaagcucu agagucggua      840

aggacccgcu acagcccggg aaaaauaggg cucucaaggc cuccgacgcu uuccagcggu      900

accgcaucgc cgcacugauc ggaaaccuua gaguuagggu ggauggcgag aaacguauuc      960

ugucaguaga ggaaaagaau uugguguuug accaccucgu caaccugaca ccuaagaaag     1020

agccagaaug ggugaccaua gccgagaucu uaggcauuga ucgggggcaa cugaucggaa     1080

cugcuacaau gaccgacgau ggugaacgag cgggcgcaag acccccuacg cacgacacua     1140

auaggagcau aguuaacagu cgcauugccc cacucgugga uugguggaag accgcuucug     1200

cccuugagca gcaugcaaug gucaaagccc uguccaaugc ugagguggac gauuucgaca     1260

gccccgaagg ggccaaggua caggcauuuu ucgcggauuu ggacgacgau guucacgcua     1320

aacuagacuc gcugcaucuc ccggugggac gggccgcaua uucugaggau acacugguca     1380

gacuuacuag gcgcaugcug uccgacggcg uggauuugua caccgcccga cuccaagaau     1440

uugguaucga gccuucaugg acacccccaa ccccucggau cggggaaccc gucggcaacc     1500

cagcugugga ccguguucug aagacgguga guagaugguu agagagcgcc acuaagacau     1560

ggggagcacc cgagagggua auuaucgaac acguccgcga gggcuucgug accgaaaaac     1620

gggcuagaga gauggauggg gacaugaggc gacgcgccgc gcggaaugcc aagcuguuuc     1680

aggagaugca ggaaaaacua aacguucagg gaaagccuuc cagagcugau cucuggaggu     1740

aucaaucugu gcagcgucag aauugccagu gugcauacug cgguagccca auuacuuucu     1800

caaacaguga gauggaccac auagucccgc gcgccggcca aggguccaca aauacccggg     1860

aaaaccuggu ggcuguaugu cauagaugca aucagagcaa aggaaacacg cccuuugcca     1920

ucugggcaaa gaacaccucu auugagggcg ugucaguuaa ggaagcgguc gagaggacac     1980

gacacugggu gacugauacc gguaugcgga guacagacuu caaaaaguuc acuaaagccg     2040

uggucgagcg cuuucagaga gcuaccaugg augaagagau cgacgccagg agcauggaau     2100

ccguugcaug gauggcuaau gagcuucggu cucgaguggc ccagcauuuc gcaucgcacg     2160

ggacaaccgu gagaguauau cgcggcagcu ugacugccga agcuaggcgu gcguccggaa     2220

ucucaggcaa gcugaaauuu uucgacgggg ucggaaagag ucggcucgau agaaggcauc     2280

acgccauuga cgcagcugug auagccuuua cgucugauua cguugccgag acacuggcug     2340

ugcgcagcaa ccuuaagcaa ucccaggcac accgacagga ggccccucag uggagagagu     2400

ucaccgguaa agacgcggaa caucgggcug ccuggagggu cuggugucaa aagauggaaa     2460

aacugucagc auugcucacu gaggaucugc gcgacgaucg ugugguugug augagcaaug     2520

uaagauuacg gcugggcaac gggucugccc acaaggaaac caucggaaaa cuauccaagg     2580

ucaaacucag uagccagcug ucuguguccg acauugauaa ggcuucaagu gaggcacuuu     2640

ggugcgccuu gacaagggag ccaggcuuug accccaagga aggucugccu gcgaauccag     2700

agcgccauau ccgagugaac gggacccacg ucuaugcugg cgauaauauu ggacucuucc     2760

ccguuagcgc cggcucgauc gcacugagag ggggauacgc cgaauuaggc uccucuuuuc     2820

accaugcucg gguguacaaa auaacuagcg guaagaaacc ggccuucgca augcugaggg     2880

uauauacaau cgaccuuuug ccuuaccgca accaggaucu cuuuucagug gagcugaagc     2940

cacaaacgau gaguaugaga caggcugaga aaaagcuaag ggacgcccug gcgaccggga     3000

augccgaaua ucucggaugg cuggucgugg acgaugagcu uguuguggac acuuccaaga     3060

uugcaacaga ucaggucaaa gcuguggaag ccgaguuggg caccauccgg cguuggcgcg     3120

uugacgguuu cuuuucuccc agcaagcugc gauuaagacc ucuccagaug uccaaagaag     3180

gcauuaagaa agagucagcu cccgagcuga gcaagaucau agauaggcca ggguggcuuc     3240

cugcagugaa caagcuguuc agugacggaa augucacagu agugcggcgc gauucuuugg     3300

gcagaguuag gcucgaaucc acugcccauc ugcccgugac cuggaaaguc caaaagcggc     3360

cagccgcuac gaaaaaggcg gggcaggcca aaaagaagaa aggacuagug caucacauuu     3420

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     3480

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     3540

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     3600

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     3720

caccagaauu                                                            3730


<210>  3598
<211>  3430
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3598
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcagccagg auucuggcgu      120

ucgacauagg aaucagcucc auugguuggg cuuuuucuga gaacgaugaa cucaaggacu      180

gcggcguucg gaucuucacc aaaguggaga auccaaagac aggggaauca cuugcccugc      240

cgcgccgauu ggcacguagu gccagaaaga gguuagcucg gcgcaaagca agacugaacc      300

aucuaaagca ccucauugcc aaugaguuua aacugaacua cgaagauuau cagagcuucg      360

acgagucccu ugcuaaggcc uacaaaggau cucugaucuc gcccuaugag uugagguuuc      420

gggcgcucaa ugaacugcug ucaaagcaag auuucgcacg cguaauacuc cauaucgcca      480

agcgaagagg cuacgacgau auuaaaaaca gcgacgauaa ggagaaaggu gcuaucuuaa      540

aggccauuaa acagaaugaa gagaagcugg caaacuauca gagugugggg gaguaccuuu      600

auaaagaaua cuuucagaag uucaaggaaa auuccaaaga auuuacuaac gucaggaaua      660

agaaagaguc uuacgaacgg uguaucgcuc agagcuucuu gaaggacgag cugaaacuaa      720

uauuuaagaa gcaacgugag uucggauuuu ccuucucuaa aaaguucgaa gaggaagugc      780

ucaguguugc cuuuuauaaa cgcgcgcuga aggauuucag ccaccuugug ggcaacugcu      840

cauuuuucac ggacgagaaa agagccccua aaaauucccc acuggcauuu auguucgucg      900

cuuugaccag gauuaucaac cuccugaaua acuuaaagaa cacagaaggc auucuguaca      960

cuaaagauga ccucaaugcc cuucugaacg agguguugaa gaaugggacc cuaacauaua     1020

aacagaccaa gaaacugcuc ggacugucag acgauuacga guuuaagggu gaaaagggca     1080

cuuauuucau cgaguuuaaa aaguacaaag aguucaucaa ggcucuuggg gagcauaacc     1140

ugagccagga cgauuugaau gaaauugcca aagacauaac acucaucaag gaugagauua     1200

aacugaagaa ggcauuagcc aaauaugacc ugaaccaaaa ucagaucgau agucuaucua     1260

agcucgaguu uaaagaccac cugaacauau ccuucaaggc ucuuaaauug guaacucccc     1320

ugaugcucga aggaaagaag uacgaugagg cguguaauga acugaaccuu aaaguugcaa     1380

uuaaugagga caagaaagau uuucugccug ccuucaacga gacguacuau aaggacgaag     1440

ugacuaaucc agucguguug cgggcuauca aagaguaccg aaaggugcuc aacgcccugu     1500

uaaagaaaua uggcaagguc cacaaaauca auauugaacu ggcacgcgag guugguaaga     1560

accauagcca gagagccaaa aucgaaaagg agcagaacga gaauuacaag gcuaaaaagg     1620

acgccgaacu agagugcgaa aaacucgggc ugaagauuaa cucgaaaaau auacuuaagu     1680

ugaggcuguu caaagagcaa aaggaauuuu gugcauauuc uggagagaag aucaaaauuu     1740

ccgaucucca ggacgagaag augcuggaaa ucgaucacau cuaccccuau ucacggaguu     1800

ucgacgauag cuacaugaac aaaguguuag uauuuaccaa gcagaaucaa gagaaacuga     1860

accagacacc uuucgaagcg uuuggcaaug acuccgcuaa guggcagaag auugaggugc     1920

uugccaaaaa cuugccaacu aagaaacaga agcgcauacu cgauaaaaau uacaaggaca     1980

aggaacaaaa gaacuucaag gauagaaauc ugaacgacac cagguauauc gcacgucuag     2040

uccugaauua cacaaaagau uaucucgacu uucugccgcu uucugaugac gagaacacca     2100

aguugaauga cacgcagaaa ggcagcaagg ugcauguuga ggccaaguca gggaugcuga     2160

ccagugcuuu acggcacaca uggggauucu ccgccaaaga ucgaaacaac caccuccauc     2220

acgcaauuga cgcugugauc auugccuacg cgaauaacag caucgucaag gccuuuucug     2280

auuucaaaaa ggaacaggag ucaaauagug cugaacugua ugccaaaaag auaagcgagc     2340

uugacuacaa gaacaaaaga aaguuuuucg agcccuuuuc cgguuucagg cagaaagugc     2400

uggauaagau cgacgaaauu uucguaucua aaccugagcg caagaaacca ucgggcgcau     2460

ugcaugaaga gaccuuucgg aaggaagagg aguucuauca aagcuacggg ggaaaggaag     2520

gcguucucaa agcucuggag cuagguaaga ucagaaaagu gaaugggaag auugucaaaa     2580

acggcgauau guuuagggug gacaucuuca agcacaagaa aacuaauaag uuuuacgccg     2640

uccccauaua uacaauggau uucgcacuga aagugcuccc uaacaaggcg guugcccgcu     2700

ccaaaaaggg agaaauuaag gacuggaucc ugauggauga gaauuacgaa uuuugcuucu     2760

cacuuuauaa agacaguuug auucugaucc agaccaagga uaugcaggag ccagaguuug     2820

uguacuauaa cgcuuucacg ucuagcaccg uauccuuaau agucucaaaa caugacaaua     2880

aguuugaaac acucagcaaa aaccagaaga uccuguucaa gaaugccaac gagaaagaag     2940

ugauugcaaa gucuaucggc auucaaaauc uuaaaguuuu cgagaaguac aucguguccg     3000

cucuggggga agucacuaaa gccgaguuuc gacagcggga ggacuucaag aaaaagcguc     3060

ccgcagccac caagaaagcu ggacaggcga agaaaaagaa aggacuagug caucacauuu     3120

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     3180

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     3240

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     3300

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3360

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     3420

caccagaauu                                                            3430


<210>  3599
<211>  3841
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3599
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaagcgac cugguucucg      120

gacuugauau ugguauaggc uccguggggg uaggaauccu gaacaaggug accggcgaga      180

uuauccauaa aaauucuagg auuuucccag ccgcgcaggc ugaaaacaau uugguccggc      240

gcacaaaccg acaaggucgu agauuagcca ggcggaagaa gcaccgcaga gugaggcuga      300

aucggcuauu ugaggaauca gggcucauca cugacuucac gaaaauaagu aucaaccuga      360

auccguacca gcuucgcguu aagggacuga ccgaugaguu gagcaacgaa gaacucuuua      420

uugcacugaa aaauauggug aagcaucgag gcaucuccua ucuggacgau gccucugacg      480

auggcaacuc gucagucggg gacuacgcuc agauugugaa agagaauagc aagcagcucg      540

aaacaaagac ucccggacaa auccaguuag agagauauca gaccuacggu cagcugaggg      600

gcgauuucac aguagaaaaa gacgggaaga aacaccggcu uauaaacguu uuuccuacca      660

gugcauaucg uuccgaggcc uugcgcauuc ugcaaacuca gcaggaguuc aauccacaga      720

ucacagauga auuuauuaac agauaccuag agauccucac cggaaagagg aaauauuacc      780

auggccccgg uaaugaaaag ucucggacgg acuacgggcg auaucgcacu agcggagaga      840

cccuggauaa caucuucggc auucuuauag gcaaaugcac auuuuacccu gacgaguuca      900

gagcugccaa ggcguccuau acugcacaag aguuuaaccu guugaaugac cucaacaauc      960

ugaccgugcc aacagagacc aagaaauuau caaaggaaca gaaaaaccag aucauuaauu     1020

acgucaagaa cgagaaagcc auggggcccg cuaagcuguu caaguauauc gccaaacucc     1080

uuaguuguga uguggcagac auaaagggau acaggauuga uaaaagcggu aaggcugaaa     1140

uccacacguu ugaggccuau cggaaaauga agacucugga aacauuggac aucgagcaaa     1200

uggaucgcga gacccuagac aagcuggcgu acgugcucac ucugaauaca gaaagagagg     1260

gcauucagga agcccuugag cacgaguucg cagauggguc uuucucccag aaacaggucg     1320

acgagcuggu ucaauuuagg aaggcuaacu caagcaucuu cggaaaaggc uggcauaauu     1380

uuagugugaa guugaugaug gagcucauuc cugaacugua cgagaccucu gaagaacaga     1440

ugacgauauu aacccgucug gguaaacaga agacaacuuc cagcucgaac aagaccaaau     1500

auaucgauga gaagcuacuc acagaagaga uuuacaaucc aguaguggcc aaaucugucc     1560

ggcaggcuau caagaucgug aacgccgcaa uuaaagaaua uggggacuuc gauaauauag     1620

uuaucgagau ggcccgagaa acuaacgagg acgacgagaa gaaagcuauu caaaagaucc     1680

agaaggcgaa uaaagaugaa aaggacgcag ccaugcugaa agcugccaac caguacaacg     1740

gcaaggcaga gcuuccgcac uccguguuuc auggacacaa acaguuggcc accaagauua     1800

gacuguggca ccaacagggc gaaaggugcc ucuauacagg gaagaccauc ucaauacaug     1860

aucugaucaa uaacaguaau caguucgagg ucgaccacau ucuuccccug agcaucacuu     1920

uugaugacuc cuuggcuaac aaagugcucg uauacgccac ggcaaaucag gaaaagggac     1980

aacgcacacc uuaucaggcg cuggauucua uggacgaugc uuggagcuuc cgggaguuaa     2040

aagccuuugu uagagaguca aagacccuga guaacaaaaa gaaggaauac cuacucacug     2100

aggaagacau uuccaaauuc gaugugagga agaaauuuau cgagcgcaau cuggucgaca     2160

cccgauacgc aagccgggug guccuuaacg ccuugcagga acauuuccgu gcucacaaga     2220

uagauacaaa agugucuguu gugagagguc aauuuaccuc acagcugagg cgccauuggg     2280

gcauugagaa gacucgggac acauaucacc accaugccgu agacgcacuc aucauugcug     2340

ccaguagcca gcugaauuua uggaagaaac agaagaacac gcuggucucc uacucugagg     2400

aucaacuuuu ggacaucgaa accggggagc ucauaucgga ugacgaauau aaagagagcg     2460

uguucaaggc gccauaccag cacuucguug auacucugaa auccaaggag uuugaagacu     2520

caauccuauu caguuaucag guggauucua aauuuaauag aaagauuagc gacgccacaa     2580

ucuacgcuac caggcaggca aaggucggaa aagauaaggc cgacgagaca uaugugcugg     2640

gcaaaauuaa ggauaucuac acucaagacg guuacgaugc uuucaugaaa auauauaaga     2700

aggacaaauc caaguuucuc auguaccgac augaccccca gaccuucgaa aaaguaauug     2760

agccuauccu ggaaaacuau ccaaauaagc agaucaacga gaaagggaag gaggugcccu     2820

guaauccguu ucuuaaguac aaagaggaac acggcuauau ucgcaaguac agcaaaaagg     2880

gaaacggccc ugaaaucaaa ucauugaagu auuacgauuc uaagcugggg aaccacauug     2940

acauaacgcc caaagauucc aauaacaagg uugucuuaca gagugugagc ccauggcggg     3000

ccgacgugua cuucaauaaa accacaggaa aguaugagau ccucggucug aaauacgcag     3060

aucuucaauu ugaaaagggc acugggaccu auaagauuuc ucaggagaaa uacaacgaca     3120

ucaagaaaaa ggagggaguc gauuccgacu cagaguucaa auuuacacug uauaagaaug     3180

auuugcuccu gguuaaagac acugaaacca aggaacagca acuauucaga uuucugagua     3240

ggacgaugcc uaagcagaaa cauuacgugg agcucaagcc cuaugauaaa cagaaguucg     3300

aaggcgguga ggcgcugauu aaagugcuug ggaacguagc caauagcggc cagugcaaga     3360

agggauuggg caaaucgaac aucuccauau acaagguccg uacagacgug cuggggaauc     3420

aacacaucau uaaaaacgag ggagacaagc caaaauuaga uuucaagcgc cccgcugcca     3480

ccaagaaagc aggccaggcu aagaaaaaga aaggacuagu gcaucacauu uaaaagcauc     3540

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     3600

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     3660

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     3780

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     3840

u                                                                     3841


<210>  3600
<211>  4399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3600
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaacccag uucgagggau      120

uuacaaaccu guaccaaguu agcaagacuc ucagguucga acuuauucca caggguaaaa      180

cgcugaagca uauacaggag cagggcuuua ucgaagagga caaggcccgg aaugaucacu      240

auaaagaauu gaagccgauu aucgaccgca uuuacaaaac cuaugcggau caaugcuuac      300

agcuggugca gcuagacugg gagaaccucu ccgcugccau cgauucuuac cgaaaggaga      360

aaacagaaga gacucguaau gcacugauag aggaacaggc caccuauaga aacgcuaucc      420

augacuacuu cauugggagg acagauaauc uuaccgacgc aaucaacaag cggcacgccg      480

agauuuauaa gggacuguuu aaagcugaau uguucaaugg caagguacuc aaacaacugg      540

guacugugac aaccacggag caugaaaacg cccugcuccg cucauuugau aaguucacua      600

ccuacuuuag uggguucuac gagaauagga aaaacgucuu uagcgcggaa gacaucucca      660

cagcaauacc ccacagaauu gugcaggaua auuucccuaa guuuaaagag aacugucaca      720

ucuucacucg guuaauuacc gccguuccau cucugcgcga gcauuucgaa aaugugaaga      780

aggcuaucgg aaucuuuguc ucgacaucaa uugaggaagu guucagcuuu cccuucuaua      840

accagcuuuu gacccagacg caaauagacc uguacaauca gcuacucggc ggcaucaguc      900

gagaggccgg gacugagaaa auuaagggac ugaacgaagu acuuaaccug gcaauccaga      960

aaaaugauga gacagcucac auaauugccu ccuugccuca uagauuuauc ccacucuuca     1020

agcaaauccu gucugacagg aacacccuua gcuuuauucu ggaagaguuc aaauccgacg     1080

aagagguuau ccagucauuu ugcaaguaua agacucuccu ucggaaugaa aacgugcugg     1140

agacagcgga ggccuuguuc aaugaacuaa acaguauuga ucugacccac auauuuauca     1200

gccacaaaaa gcucgagacg auuucuuccg cacuguguga ccauugggau acccuucgua     1260

augcucugua cgaacgcaga aucucagagu ugacagguaa aaucacuaag agcgccaaag     1320

agaaggucca gaggagucuc aagcacgaag acauuaaccu gcaggagaua aucucugcug     1380

ccggcaaaga auuauccgag gcauucaagc agaaaaccag cgaaauucug ucgcaugccc     1440

acgcugcgcu agaucaaccc cucccuacaa cucugaagaa acaggaggag aaggaaaucc     1500

uuaagucuca guuggacucc cugcucgggc uguaucaucu ucuggauugg uuugcagugg     1560

acgagucaaa ugaaguggau ccagaguuca gugcccgguu gaccggaauu aaacucgaaa     1620

uggagccgag ccuguccuuc uacaacaagg cucgaaauua ugccacuaaa aagcccuacu     1680

cugucgagaa auuuaaguua aacuuccaaa ugccuacccu ggcaagcggc ugggacguua     1740

auaaagaaaa gaacaauggu gccauccuau uugugaagaa cgggcucuac uaucugggaa     1800

uaaugccaaa acagaagggc cgcuacaaag cucuuucauu cgagcccacu gaaaagacga     1860

gugagggcuu ugauaaaaug uauuacgacu auuucccuga cgccgcaaag augaucccaa     1920

agugcuccac acaguugaaa gcgguaaccg cucacuuuca gacucacacc acacccauuc     1980

ugcucagcaa caauuucauc gaaccgcugg agauuaccaa ggagaucuac gauuuaaaca     2040

auccugaaaa agagcccaag aaauuucaaa cugccuaugc aaagaagaca ggggaccaga     2100

aaggauacag agaagcccug uguaagugga uagauuucac gagggacuuu cuuucuaaau     2160

acaccaagac uacaucaauu gauuugagua gccuccggcc auccucucag uauaaagacc     2220

ugggugagua cuaugcugag cuaaacccuc ugcucuacca uaucucguuc cagcgcauug     2280

ccgaaaagga gaucauggau gcaguggaaa ccggcaagcu guaucuuuuu caaauauaca     2340

auaaagacuu cgcuaagggg caccauggaa aacccaacuu gcacacacug uauuggacug     2400

gcuuauucag cccagagaau cucgccaaga ccuccaucaa acugaacggu caggcggaau     2460

uauuuuacag acccaaguca aggaugaaac guauggccca ccggcugggg gagaagaugu     2520

ugaauaagaa acucaaggau cagaaaacgc cuauuccaga cacccuguac caagagcuau     2580

augauuacgu caaccaucga cugagucacg accucucuga ugaagcuaga gcacugcuuc     2640

cgaaugugau cacaaaggag guuagccaug aaauuaucaa agacaggcgc uucacuuccg     2700

acaaguuuuu cuuucacgug cccauaaccu ugaacuauca ggccgcuaau ucaccuagca     2760

aguucaacca gcgggucaau gccuaccuga aagagcaucc agaaacaccc auuaucggca     2820

ucgauagagg agagaggaac uuaauuuaua ucacugugau ugacucuacc ggcaagauac     2880

ucgagcagcg cucccugaac acgauccaac aguuugauua ccagaaaaag cuggacaauc     2940

gagaaaaaga gcggguagca gcgcgucagg ccuggagugu uguggggaca auuaaggauc     3000

uuaagcaagg auaucuaagc caggucaucc acgaaauugu ggaccucaug auccacuacc     3060

aggcugucgu gguucuggag aacuugaauu ucgguuuuaa aucuaagaga accggcauag     3120

ccgaaaaagc aguguaccag caauucgaga agaugcugau cgauaaauua aacugccucg     3180

uacugaagga cuauccugcu gagaaggucg ggggagugcu uaauccauac cagcugacug     3240

aucaguuuac auccuucgcc aaaaugggca cccaaucagg uuucuuguuu uauguucccg     3300

caccguacac aaguaagauu gacccucuca ccggguucgu ggauccauuu gucuggaaaa     3360

caaucaagaa ccaugaaagc aggaaacacu uccuggaggg cuuugacuuc cuacauuaug     3420

augugaagac gggagacuuu auucugcacu ucaagaugaa ucgcaaccuu ucguuucagc     3480

ggggccuccc cggguucaug ccugccuggg acaucguauu ugagaaaaau gaaacccagu     3540

ucgaugcuaa gggaacuccc uuuauagcgg guaaaagaau ugugccaguu aucgagaacc     3600

auagguucac aggccgauac cgcgaccugu auccugccaa ugaauugauc gcacuguuag     3660

aggaaaaggg gauugucuuc cgggauggau ccaacauacu ccccaaacug cuugagaaug     3720

acgauucuca cgcuaucgac accauggugg cccugauuag aagcguguug cagaugagga     3780

acucaaaugc cgcuacaggc gaagauuaca ucaacagucc aguccgugac cucaacggug     3840

uuuguuuuga uucccgcuuc caaaauccgg aguggccuau ggacgcagau gccaacgggg     3900

cguaccacau ugcucugaag ggccagcuac ugcuuaauca ucucaaagag ucuaaggacc     3960

ugaaguugca gaacggaauc agcaaucagg acugguuagc cuauauacaa gaacugcgga     4020

acaaaagacc cgcagccacu aagaaagcug gccaggcaaa gaaaaagaag ggacuagugc     4080

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     4140

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     4200

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     4260

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     4380

uuucagagcc accagaauu                                                  4399


<210>  3601
<211>  4378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3601
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaagcauu uaccaggagu      120

ucguuaacaa guauucccug ucuaaaaccc ucagguuuga acuuauacca caaggaaaga      180

cacuggagaa uaucaaggcc cgggguuuga uuuuagacga ugaaaaacgc gcgaaggacu      240

acaaaaaggc uaaacagauc auugauaagu aucaucaguu cuuuaucgag gaaauacugu      300

caagugugug caucagcgag gaccuacucc agaacuacuc cgauguauau uucaagcuga      360

aaaagucuga cgaugacaau cuucaaaaag auuuuaaguc ggccaaagac acuauuaaga      420

aacagaucuc agaguacauu aaggauagcg aaaaguucaa aaaccuguuu aaucagaacu      480

ugaucgacgc aaagaaaggc caggagagug aucucauacu guggcugaag caauccaaag      540

acaaugggau ugaacucuuc aaggccaacu cugacaucac ggauauugac gaggcuuuag      600

agaucaucaa gagcuuuaaa ggauggacca cauauuucaa gggcuuucac gaaaaucgua      660

aaaacgugua cuccucaaau gauauuccga caaguauaau cuaccgaauu gucgacgaua      720

accugcccaa guuccuugag aauaaagcaa aguaugagag cuugaaggac aaagccccug      780

aggcuaucaa cuacgaacag auaaagaaag aucuggccga ggagcuaacc uuugacauug      840

auuauaagac aucugaagug aaucagagag uuuucucccu cgacgaggug uucgaaaucg      900

cgaacuuuaa caauuaccug aaccagucag guaucaccaa auucaauacu auuaucgggg      960

gaaaguuugu caacggcgag aauacaaaga ggaaaggcau uaacgaauau auaaaucuuu     1020

acagccaaca gaucaacgau aagacccuga aaaaguauaa aaugagugug uuguucaagc     1080

agauucucuc ugacacggag uccaagagcu uuguaaucga caaacuggag gaugacucgg     1140

auguugugac uaccaugcaa ucuuucuacg aacagaucgc agccuuuaag acagucgagg     1200

aaaaauccau uaaggagacu uuaucacucc uguucgacga ucuuaaagcu cagaagcugg     1260

acuugaguaa aauauacuuu aagaaugaua agagccuaac cgaccugucc cagcaagugu     1320

ucgaugacua uucugugauc gggacagccg uccucgaaua cauuacccag cagaucgcac     1380

ccaaaaaccu ggauaaucca agcaagaaag agcaggagcu uauugcuaag aaaacggaaa     1440

aggccaagua ucugucauug gagacuauca aacucgcgcu ggaagaguuu aacaagcauc     1500

gggacauaga uaaacaaugu cgcuucgagg aaaucuuagc caauuuugca gcuauuccua     1560

ugaucuucga cgagauugcc cagaacaagg acaaucuggc ucagaucagu auaaaauacc     1620

agaaccaagg aaagaaggau cuacuccagg ccuccgcaga agacgauguu aaagccauua     1680

aggaccugcu ugaucagaca aacaauuugc ugcacaaacu caagaucuuc cauauuagcc     1740

agucugagga caaagcuaac auccuggaua aggacgaaca cuuuuaucuu guguucgagg     1800

agugcuacuu ugaacuggcg aauauaguac cauuguauaa caaaaucaga aauuacauua     1860

cccaaaagcc cuacucagau gagaaguuca aacucaacuu ugaaaauagu acucuggcaa     1920

acgguuggga caagaauaaa gagccugaua acacagccau cuuauucauu aaggacgaua     1980

aauauuaccu gggcgugaug aauaagaaga acaauaaaau cuuugacgac aaggcuauaa     2040

aagaaaacaa gggggaggga uauaaaaaga uugucuacaa gcuacuccca ggcgccaaua     2100

aaaugcugcc gaagguguuc uuuagcgcaa aauccaucaa guucuauaac cccucugagg     2160

auauccuuag gauucggaac cacucgaccc auacgaaaaa ugguagcccu cagaaggggu     2220

acgaaaaguu ugaguucaac aucgaagacu gucgcaaauu uauugauuuc uauaagcagu     2280

ccauaucaaa acacccagag uggaaggacu ucggauuucg auucagugau acccaaagau     2340

acaauucuau cgacgaauuu uacagggagg uugagaacca gggcuauaaa uugacauucg     2400

aaaauauuag cgaguccuac aucgauucag uggucaacca gggcaagcug uaucucuuuc     2460

agauuuacaa uaaggacuuc agcgccuauu cuaaagggcg gcccaaccug cauacuuuau     2520

acuggaaggc ucuguuugau gaacguaauc uucaagacgu gguauauaaa uugaacggag     2580

aggccgaacu cuucuaccgc aagcagucca ucccuaaaaa gauaacccac ccagcaaaag     2640

aagcgaucgc uaauaagaac aaggauaauc ccaaaaagga gaguguuuuu gaauacgacc     2700

ugauuaaaga caagagauuc acagaggaua aauuuuucuu ccacugcccg aucacuauua     2760

acuuuaagag cucuggugcc aauaaguuca acgacgaaau caaccuacug cucaaagaga     2820

aggcaaauga ugugcauaua cuguccauug acaggggcga gcggcaccuu gccuauuaca     2880

ccuuggucga ugggaaagga aacaucauca agcaggacac auuuaauauu auaggcaacg     2940

aucgaaugaa aaccaauuau caugacaagc uggcugccau cgaaaaggau cgcgacucag     3000

caagaaaaga uuggaagaaa auuaacaaua ucaaggagau gaaagaaggu uacuuaaguc     3060

agguggucca cgagauugcu aagcucguga ucgaauauaa cgccauaguu guguucgagg     3120

accugaauuu uggguucaag aggggccggu uuaaaguaga gaagcaaguc uaccagaaac     3180

uugaaaagau gcugaucgag aaauugaacu aucucguguu caaggauaau gaauuugaca     3240

aaacuggagg cguucugcgc gcguaccagc uaacggcccc uuucgagaca uuuaagaaga     3300

uggggaaaca gaccggaauu aucuacuaug ugcccgcugg uuucacuagc aagauuuguc     3360

cagucaccgg cuuugugaac caacuguacc cuaaauauga aucgguaucc aagucucagg     3420

aguucuucag caaauuugac aagaucugcu acaaucucga uaagggguau uucgaguuuu     3480

cauucgacua caaaaacuuu ggagauaagg cagccaaagg caaguggaca auagcuaguu     3540

ucgguuccag acugauuaau uuuaggaacu cugacaaaaa ucauaacugg gauacccgug     3600

aaguguaucc cacuaaggag cuugaaaagu ugcugaaaga cuacagcauc gaguacgggc     3660

acggcgagug uaucaaggcc gcaauuugcg gagaauccga uaaaaaguuc uuugcgaaau     3720

uaacaucagu ucucaacacg auccugcaga ugcggaauag caagaccggc acugagcuug     3780

acuaucugau aaguccaguc gccgauguga acgggaauuu cuuugacucu cgacaagcuc     3840

ccaagaacau gccucaggau gccgacgcaa auggagcuua ccacauuggu cuaaaaggcc     3900

ucaugcuguu ggggagaauc aagaacaauc aggaaggcaa aaagcugaac uuagugauua     3960

aaaaugagga auauuucgag uuuguccaga acaggaauaa caagcgccca gccgcaacua     4020

aaaaggccgg acaagcuaag aaaaagaaag gacuagugca ucacauuuaa aagcaucuca     4080

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     4140

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     4200

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     4320

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       4378


<210>  3602
<211>  4484
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3602
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaga caagaaauac agcauuggac uggauauagg uacaaacucc guuggcuggg      180

ccgugaucac agacgaguau aagguaccau cuaagaaauu caaggugcuc gggaauacug      240

auaggcauuc aauuaaaaag aaccuuaucg gagcgcuguu guuugacagu ggcgaaacgg      300

cugaggccac ccgguuaaaa cgcacagcac gacguagaua cacuaggcgg aagaaucgca      360

uuugcuaucu gcaggaaauc uucagcaacg agauggccaa ggucgaugac uccuuuuucc      420

acagacuaga agagucuuuu cucguggagg aagauaaaaa gcaugagagg cacccgauau      480

ucgguaauau cguugacgaa guggcuuacc augagaaaua ucccaccauu uaccaucugc      540

ggaagaaacu ugucgauucg acagacaagg cagaucugcg cuugaucuau cucgcccugg      600

cucacaugau uaaauuucga gggcacuucc ugaucgaggg agaccucaac ccugauaauu      660

cagacgugga caaguuauuu auacaacugg uacagaccua caaccagcuu uucgaagaga      720

auccaauuaa cgccagcggc guugaugcga aggcaaucuu gagugccaga cuguccaaau      780

cuaggcggcu agaaaaucuc auugcucagc ugcccggcga gaagaaaaac gggcuuuuug      840

gaaaucugau cgccuugagc cucggucuga cuccuaacuu caaguccaau uuugacuuag      900

cagaagaugc uaaacugcaa cucucaaagg acacauacga ugacgaucuu gacaaccugu      960

uggcccagau cggcgaucag uaugcggacc uauuccuggc cgcaaagaac cucagugaug     1020

cuauucugcu uagcgacaua cugcguguga auaccgagau cacgaaagcc ccauugucug     1080

cuuccaugau uaagcgcuac gacgagcacc aucaggaucu cacucuguua aaagcccugg     1140

ucagacaaca gcuacccgaa aaguauaaag agaucuucuu ugaccaguca aagaacgggu     1200

acgcaggaua uauagauggc ggugccagcc aggaagaguu cuacaaguuu auuaaaccua     1260

uccucgaaaa gauggacggg accgaggagc ugcuugugaa auugaauagg gaagaucugc     1320

uccggaagca acgaacauuc gacaacggaa guaucccaca ccagauucac cugggcgagc     1380

uucaugcuau ccugcgcaga caggaagauu uuuauccguu cuugaaagac aauagggaga     1440

agauugaaaa gauacucacu uuucggaucc ccuacuacgu gggcccucug gcgcgcggga     1500

acucuagauu cgcauggaug accaggaaau ccgaggagac aauuacccca uggaauuuug     1560

aagaggucgu ugauaaggga gccagcgcuc aaucguucau cgaacguaug acgaacuuug     1620

acaaaaauuu acccaacgag aaggugcugc cuaaacacuc ucuacucuau gaguacuuca     1680

cuguauauaa ugaacugaca aaggugaaau acgucaccga ggguaugcgg aagccagccu     1740

uucuuuccgg cgaacagaag aaagcaaucg uggauuugcu guucaagacu aaccgaaaag     1800

uuacagugaa gcagcucaaa gaggacuauu ucaagaagau ugaauguuuu gauucagucg     1860

agauaagugg gguggaggac agauucaaug ccagccuggg aaccuaccau gacuuacuga     1920

aaaucauuaa ggauaaagac uuucuugaua acgaagagaa ugaagacauc uuggaggaua     1980

uuguacucac gcugacccua uucgaagaca gggagaugau cgaggaacgc cugaagacau     2040

augcucaccu cuuugaugac aaaguuauga agcagcugaa gcggagaagg uacacuggcu     2100

ggggucgccu uucccgaaaa uugauaaacg ggauccggga uaagcaaucu ggcaaaacca     2160

uucuggacuu cuuaaagagc gauggauuug ccaaccguaa uuucaugcag cucauccaug     2220

acgauucacu gacauuuaaa gaggacauuc agaaggcaca ggugaguggc caaggggacu     2280

cccuucacga acacaucgcg aaccuggcug gaagccccgc cauaaagaaa gguauuuugc     2340

agacugucaa gguggucgau gagcucguga aaguuauggg cagacauaag ccggaaaaua     2400

ucgugauuga gauggcaagg gagaaccaga ccacacagaa agggcaaaag aacucucgcg     2460

aacggaugaa aagaaucgag gaaggaauaa aggagcuggg cucacagauc cuaaaggagc     2520

acccuguaga aaauacccag cugcagaaug agaaacucua ccuguauuac cuucaaaacg     2580

guagggacau guaugucgau caggaauugg acauuaaucg acugagugau uacgacgugg     2640

aucauaucgu uccccagagc uucuuaaagg acgauuccau ugacaacaaa gugcucacuc     2700

gcucugauaa gaaucggggg aaaucggaca acgucccaag cgaggaagug guaaagaaga     2760

ugaaaaauua uuggagacaa cugcuuaacg ccaagcugau cacgcagagg aaauuugaca     2820

aucuaacaaa ggcugagcgu ggcggacucu ccgagcugga uaaagccggc uucauaaagc     2880

gccaguuggu ggaaacccgg cagauuacua agcacguugc acaaauccug gacucaagaa     2940

ugaacaccaa auacgaugag aacgacaagu uaaucaggga agucaaagug auuacacuca     3000

agaguaaacu ggugucugau uuucgaaagg acuuccaguu cuauaagguc cgggagauca     3060

auaacuacca ccaugcucac gaugccuacc uuaaugcggu uguggggacc gcccugauua     3120

aaaaguaucc uaaauuggaa agcgaguuug uguacggaga cuauaaggua uacgaugucc     3180

gcaaaaugau agcuaagucc gagcaggaaa ucgguaaggc aacugccaaa uauuucuuuu     3240

acucaaacau uaugaauuuc uuuaagacag agaucacgcu cgcuaacggc gaaauuagaa     3300

aaaggccccu gaucgagacc aauggggaaa cuggagagau agugugggac aagggccggg     3360

auuucgccac aguucgaaaa gugcuaagca ugccacaggu caacaucgug aagaaaaccg     3420

agguucaaac agguggguuu ucuaaggaau ccauucugcc caagagaaau agugacaaac     3480

uuaucgcacg caagaaagau ugggacccua agaaauaugg cggauucgac agcccaacug     3540

uggcguacuc uguacucguc guggccaagg uggagaaggg caaauccaag aaacugaagu     3600

cagucaaaga auugcugggg auuaccauca uggagaggag uagcuuugag aagaacccga     3660

uagauuucuu agaagcuaag ggauacaaag agguuaagaa agaccucauu aucaagcugc     3720

ccaaauauuc gcuuuuugaa cuggagaaug gccguaagcg gauguuggcc uccgcuggug     3780

aacuccagaa ggggaacgag cuggcacuac cuucuaaaua cgugaauuuc cuguaucuug     3840

ccagccauua cgagaagcuc aaaggaucac cagaagauaa cgagcagaag cagcuguucg     3900

uagaacaaca caaacauuau uuggacgaga ucauugaaca gauaagugag uuuuccaaga     3960

gagugaucuu agcagaugcc aaucuggaca aaguccucuc ugcuuacaac aagcacaggg     4020

auaagcccau ucgcgagcag gcggaaaaca ucauucaccu guucacgcuu accaaucugg     4080

gcgccccugc agcuuuuaaa uauuucgaca caacuaucga ucgaaagaga uacaccagca     4140

caaaagaggu guuggacgcc acucuaauac aucaauccau caccggucug uacgaaacgc     4200

ggauugaucu cucacagcug ggcggggaca agaggccagc cgcuaccaaa aaggcaggac     4260

aggccaagaa aaagaaauaa ggacuaguaa uaaggaaacu guaugaaugu cugugggcag     4320

gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu     4380

uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4484


<210>  3603
<211>  5267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3603
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa cuucaagauu cugccaauag ccaucgaccu cggaguuaaa aauaccggug      180

uguuuagcgc guucuaccag aagggcacau cccuugagag gcuggauaac aagaauggga      240

aaguauauga auugucuaag gacucauaca cuuuacugau gaacaaucgg acggcucgcc      300

gacaucaacg uagaggaauu gauaggaaac agcuagugaa gcggcucuuu aaacugaucu      360

ggaccgagca gcuuaaccug gaaugggaca aggauacaca gcaagccauu aguuucuugu      420

uuaaucgcag aggcuucagc uuuaucacug acgguuauuc cccggaguac cucaacauag      480

uccccgaaca ggugaaggca auccugaugg auauuuucga cgauuauaau ggggaggacg      540

aucuggacuc uuaccucaaa uuagccaccg agcaggaauc gaagaucuca gagauuuaua      600

acaaacugau gcagaagauc cuugaauuua aauugaugaa gcugugcaca gauauaaaag      660

acgacaaggu uagcaccaag acucuaaaag agauuacaag uuacgaguuc gaacuccugg      720

cugauuaccu ugcaaauuau uccgagucuc ugaagaccca aaaauuuagc uacacggaca      780

agcagggaaa cuugaaagaa cucuccuauu accaccauga uaaguauaau auccaggagu      840

uccugaagag gcacgccacu auuaacgacc ggaucuuaga uacccugcuc acagacgauc      900

uugacaucug gaauuuuaac uucgaaaaau ucgauuuuga caagaacgag gagaaacugc      960

agaaucaaga agauaaggac cauauucagg cucacuugca ccauuucgug uuugccguca     1020

acaaaauaaa gucagagaug gcgaguggcg gccgccaccg aagccaguac uuccaagaaa     1080

ucacuaaugu gcuagacgag aacaaucauc aggaagggua ucugaagaac uuuugugaga     1140

aucuccacaa caaaaaguac ucuaaucugu ccguuaaaaa ccuuguaaau cugauuggaa     1200

acuugucaaa ucucgagcug aagccuuuaa gaaaauacuu caacgauaag auccacgcaa     1260

aggccgacca uugggaugaa cagaaauuua ccgagacaua uugccacugg auacugggug     1320

aauggagggu gggcgucaag gaccaggaua aaaaggacgg ggcuaaauac agcuauaagg     1380

aucuauguaa ugagcucaaa caaaagguga ccaaggccgg acugguggac uuccuuuugg     1440

aacuggaucc augccggacg auucccccuu accucgacaa caacaaucgu aaaccaccca     1500

agugucagag ucugauccuu aacccuaaau uucuggauaa ucaguaucca aacuggcagc     1560

aauacuugca ggagcucaag aaacugcagu cuauccagaa uuauuuagac uccuucgaga     1620

cugaucugaa gguccuaaag agcucgaaag accaaccgua cuuuguugaa uacaagucuu     1680

ccaaccagca gauugcauca ggccagcgcg acuauaaaga ucucgacgcu agaauccugc     1740

aauucauuuu ugauagggug aaggccagug acgagcuuuu gcugaaugaa auauacuucc     1800

aggcgaaaaa gcucaagcag aaagccagcu ccgagcugga gaaguuagaa ucuagcaaaa     1860

agcuggauga gguaaucgca aacucacaac uuagucagau uuugaaaucc cagcauacaa     1920

augguaucuu cgaacagggg accuuucucc accuggugug caaguauuac aagcaacggc     1980

agcgagcucg cgacagcaga cuauauauca ugcccgagua cagguaugau aaaaagcugc     2040

auaaauacaa caauacugga cgguucgacg augacaacca gcuccugaca uacuguaauc     2100

acaagccucg ccagaaaaga uaucaacuuu ugaacgaucu ggccggcguc uuacaggugu     2160

cuccaaauuu ucucaaggac aaaauuggcu cagaugacga ccuguucaua aguaaguggc     2220

uuguugaaca caucaggggg uuuaagaaag caugcgagga uagccugaag auucagaaag     2280

acaaccgugg auugcucaac cauaagauca auauugcccg gaacaccaaa gguaagugug     2340

agaaggaaau cuucaaucug auaugcaaaa ucgagggcuc cgaagauaag aaagggaacu     2400

acaagcacgg acuagcauau gagcugggcg ugcuccuguu uggugaaccc aaugaggccu     2460

cuaaaccuga guucgaccga aagauuaaga aauuuaacuc gaucuacagc uucgcucaga     2520

uucaacagau cgcguuugca gaaagaaagg ggaaugccaa cacgugugcu gucugcuccg     2580

ccgauaaugc acauaggaug cagcaaauaa aaauuaccga gccaguggaa gacaacaagg     2640

auaaaaucau ucuuucagcc aaggcucagc gcuugcccgc caucccgaca cggauaguag     2700

acggcgcagu uaagaaaaug gcgacuaucc uggcuaagaa uauuguggau gacaacuggc     2760

agaauaucaa acaggucuua agugccaagc accaacucca cauuccuauc auaaccgagu     2820

cuaacgcauu cgaauuugag cccgcccugg cugaugugaa aggaaagagc cuuaaggaca     2880

gaaggaaaaa ggcccuggag cgcauuuccc cagaaaauau cuucaaagac aagaacaacc     2940

gaaucaaaga guucgcaaag ggcauuucag cuuauagcgg ggccaaucua acagauggag     3000

acuuugaugg ugcgaaagaa gaacucgacc auaucauucc ucggucucac aagaaguacg     3060

gcacucugaa cgaugaggcc aauuugauau gcgucacccg uggggacaac aaaaauaagg     3120

gaaacagaau cuucugucug agggauuuag cugacaauua uaaacucaag caguuugaaa     3180

caaccgauga ccuggagauu gagaaaaaga ucgcagauac uauuugggac gccaacaaga     3240

aagauuucaa guuuggcaau uaccgcuccu ucaucaaccu uacgccccag gagcagaagg     3300

cuuuucggca ugcccuguuc uuggcagacg aaaauccaau aaaacaagcg gugaucagag     3360

ccauuaacaa uaggaaccga acauuuguua augguaccca gcgcuacuuc gcugaggugc     3420

ucgccaacaa uaucuaucug cgggcuaaaa aggagaaccu aaacacugac aagauuaguu     3480

uugauuacuu cgggaucccc accauaggca auggaagagg cauugcugaa aucaggcagc     3540

uguaugagaa aguagacagc gauauccagg ccuacgcaaa gggggacaaa ccucaagccu     3600

cuuauuccca ccuuauugau gcuaugcucg cguucugcau agccgcagac gaacaucgua     3660

acgauggauc aaucggucug gagauugaca agaauuacag uuuguaucca cuggauaaaa     3720

acacaggcga agucuuuacc aaggacaucu ucagccagau uaagaucacu gauaaugagu     3780

uuucggacaa aaaguuagug cgcaaaaagg cuauagaggg guucaacaca caccggcaga     3840

ugacgagaga uggaaucuac gccgaaaauu accucccgau ucugauccac aaagagcuua     3900

acgaaguuag gaagggcuau accuggaaaa auuccgagga aauuaagauc uuuaagggua     3960

aaaaguacga cauacagcaa cugaacaauu ugguguauug ucucaaauuc gucgacaagc     4020

ccauuucuau cgauauccag auuagcacuc uggaggagcu acgaaacauc cugacaacca     4080

auaacauagc cgcuacagca gaauacuauu acauuaaucu uaaaacucag aagcuccaug     4140

aguauuacau cgaaaacuac aacaccgccc ugggguauaa gaaauacuca aaggagaugg     4200

aguuuuugcg gaguuuagcg uaucgcuccg aaagagugaa aauuaagucu aucgacgaug     4260

uaaaacaagu gcuggacaag gauagcaauu ucauaauugg caagaucacg cucccuuuua     4320

aaaaggagug gcagaggcug uaccgggaau ggcagaacac cacaaucaaa gacgauuaug     4380

aguuccuuaa guccuuuuuc aauguuaaau caauuacuaa gcugcacaag aaaguccgaa     4440

aggacuuuag cuugccaauc aguaccaacg aaggaaaauu ccuagugaag agaaaaacau     4500

gggauaauaa cuucauuuac cagauacuga augacucuga uucccgcgcu gacggcacua     4560

agcccuuuau cccugccuuc gacauuagca aaaacgagau cguggaggca aucauugauu     4620

cguuuaccuc aaagaauaua uucuggcucc caaagaacau cgaacugcaa aaagucgaca     4680

auaagaacau uuuugccauc gauacgucua aaugguucga gguugaaaca cccagugacc     4740

uuagggauau ugggaucgcu accauacagu auaagaucga caauaacucc cguccgaaag     4800

ugcgggugaa guuagauuac guaauugacg augacagcaa gaucaauuac uuuaugaacc     4860

auucucugcu caaaagcaga uauccugaua aggucuugga gauucugaaa caguccacua     4920

ucauagaguu cgaaucaagu ggauuuaaca agacaauuaa agagaugcuc ggcaugaagc     4980

uggcagguau cuacaaugaa accagcaaca auaagaggcc agccgcgacu aaaaaggcug     5040

ggcaggccaa aaagaaaaag uaaggacuag uaauaaggaa acuguaugaa ugucuguggg     5100

caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug aauaauaaac     5160

uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa aaaaaaaaaa     5220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   5267


<210>  3604
<211>  3539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3604
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaggaacuac auucugggac ucgacauagg uaucacauca guuggcuaug      180

ggauuaucga uuacgagaca cgggacguga uugaugccgg aguacgccuu uucaaagaag      240

cgaaugugga gaacaaugaa ggccgacguu ccaagagagg ugcuaggcgg cugaagcgca      300

gaaggcggca ucgcauccag cgagucaaaa aguuguuauu ugacuauaac cugcuaacug      360

aucacucuga gcucucaggg auaaauccau acgaagccag agugaaagga cugagucaaa      420

aacuuagcga ggaggaguuc uccgcagccc uguugcaucu cgcuaaaagg cggggcguuc      480

acaacgugaa ugaagucgaa gaggacacgg gcaacgagcu gucuaccaag gaacagaucu      540

cgcguaauuc aaaggcacug gaggaaaaau auguggccga gcuccaguua gaacgccuga      600

agaaagaugg ggagguaaga ggaagcauua acagguuuaa gacaagugac uacguuaaag      660

aggcuaagca gcuuuugaaa gugcaaaagg ccuaucauca gcuggaucag uccuucaucg      720

acacuuacau ugaucuacuc gaaacccggc gaacauacua ugaggguccg ggcgaagggu      780

cucccuuugg auggaaggac aucaaagagu gguacgaaau gcugaugggc cacugcaccu      840

auuucccuga ggagcuucgc agcgucaagu acgcguauaa ugcagaucug uacaacgccu      900

ugaaugaccu caacaaucug gugauaacua gagacgaaaa cgagaaauua gaauauuacg      960

agaaguuuca gauuaucgaa aacguguuca aacaaaagaa gaaaccaaca cugaagcaga     1020

uugcuaaaga gauccucguc aaugaggaag auaucaaggg uuacaggguu accuccacgg     1080

ggaaacccga guuuacuaac cuuaaggugu aucacgacau uaaggauaua accgcccgga     1140

aagaaaucau ugagaaugca gagcuguugg accagaucgc uaagauacua acaauuuacc     1200

agucaaguga agauauccaa gaggaacuga cuaaccucaa uagcgagcug acccaggaag     1260

agaucgagca gauuucuaac cuuaaaggau auacaggcac ccauaaucug uccuugaagg     1320

ccaucaaccu cauucuggac gaauuauggc acacgaauga uaaccaaaua gcgaucuuca     1380

aucgccugaa acuuguaccu aagaaggugg accucucaca gcagaaagag auuccaacua     1440

cacuggucga ugacuuuauc cuuagccccg ugguuaagag aaguuucauc cagucuauua     1500

aagugauaaa cgccaucauu aagaaauacg gcuugccuaa ugauaucauu aucgaacugg     1560

caagggagaa gaacuccaaa gacgcucaaa agaugauaaa ugaaaugcag aagcguaacc     1620

ggcagaccaa cgagcgaauc gaggaaauua ucagaacuac agggaaagag aaugccaagu     1680

aucucauuga aaagaucaag cugcaugaca ugcaggaggg aaaaugucuu uacagccugg     1740

aagcuauacc auuggaggau cuccugaaca auccguuuaa cuaugagguc gaccacauua     1800

uccccagguc ggugucuuuc gauaauuccu ucaacaauaa gguauuaguu aagcaagaag     1860

agaacucaaa gaaggguaau cgcaccccuu uucaguaccu gaguagcucc gacucuaaaa     1920

uuagcuacga aacguucaag aaacacaucc uaaaccucgc caagggcaag gggcggauau     1980

caaaaaccaa gaaagaguau cugcuugagg aaagagauau caauagguuu agugugcaga     2040

aggacuucau uaaccgcaau uuggucgaua cacgauacgc aacucgggga cugaugaacc     2100

uccugcguuc cuauuuuaga gugaauaacu uagacgucaa agugaaaagc aucaacggcg     2160

guuucaccuc uuuucugagg cgcaagugga aauucaagaa agagcggaau aagggguaca     2220

aacaucacgc cgaagaugcu cuuauuaucg cgaacgcaga cuuuauauuc aaggagugga     2280

aaaaguugga uaaggccaaa aagguuaugg aaaaucagau guuugaggag aaacaagcug     2340

aaucaaugcc agagauugaa acagagcagg aauauaagga gaucuucauc acuccccauc     2400

agauuaaaca caucaaggac uuuaaggauu acaaauauag ucauagagug gacaagaaac     2460

cuaacaggga gcucauuaau gacacccugu acagcacacg aaaggaugac aaaggcaaca     2520

cccuaauagu aaauaaccug aauggacucu acgauaagga caacgauaag cugaaaaagc     2580

uaaucaauaa auccccagaa aaguugcuga uguaucacca cgacccccag acuuaccaaa     2640

aauuaaagcu cauuauggag caguauggcg augaaaagaa cccgcuguac aaauauuacg     2700

aggaaacggg gaauuaucuu acaaaguacu cuaaaaagga caacggaccu gucaucaaaa     2760

agauuaagua cuaugguaau aaacugaacg cccauuugga uaucaccgac gauuacccca     2820

acucgcgcaa uaaggugguu aaacucagcc ugaagccaua ucgguucgac guguaccuag     2880

auaacggcgu cuauaaauuc gugacuguaa agaaucugga cgugaucaaa aaggagaacu     2940

acuaugaggu uaauuccaag ugcuacgaag aggccaaaaa gcucaaaaag auaucaaacc     3000

aggcagaauu uauugcuagu uucuacaaua acgaccugau caaaauuaau ggggagcuuu     3060

auagagucau cggagugaac aaugauuugc ugaacaggau agaggugaau augauugaca     3120

ucaccuaccg ugaauauuua gagaacauga augauaagcg cccuccccgg aucauuaaga     3180

caauagccuc uaaaacccaa agcaucaaga aauacuccac ugacauucuc ggcaaccugu     3240

augaagucaa gagcaaaaag cacccacaga ucauuaagaa agguaagaga cccgcagcga     3300

ccaaaaaggc ugggcaggcc aaaaagaaga aauaaggacu aguaauaagg aaacuguaug     3360

aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa uuaugucugc     3420

ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      3539


<210>  3605
<211>  4607
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3605
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcacu guucaacaag ugcauuauaa ucagcauuaa ucucgacuuu uccaacaaag      180

agaaguguau gaccaagcca uacucuaucg gacuugauau ugguacaaau ucaguuggcu      240

gggccgugau cacugacaac uauaaaguac cgaguaagaa aaugaaggug cuggggaaua      300

cgagcaaaaa guacauaaag aaaaacuugu uaggaguccu gcuauucgau uccggcauca      360

ccgcggaagg uaggcggcuc aagcgcacag cucgacguag auauacuagg cggcgcaaua      420

ggauucugua ccuucaggag aucuuuucua ccgaaauggc cacacuggac gaugcauucu      480

uucaaagauu ggacgauucg uuccucgugc ccgacgauaa acgggacuca aaguauccua      540

uuuuugggaa ccugguugag gaaaaagugu accaugauga guucccaacc aucuaucauc      600

ugcgcaagua ccucgccgac agcacuaaaa aggcugauuu acgacugguc uaccuugcau      660

uggcccacau gauaaaguau agaggacacu uucugauuga gggcgaguuc aauaguaaaa      720

acaaugacau ccagaagaac uuucaggacu uccuagauac auacaaugcu auuuuugagu      780

ccgaccucuc ucuggaaaac agcaaacagc uugaggagau cgugaaggau aaaaucucca      840

agcuggaaaa gaaagacagg auuuugaaac ucuuccccgg cgagaaaaau ucagggauau      900

ucagugaauu ucugaaguua aucguaggaa accaagccga uuuccggaaa ugcuuuaacc      960

uggacgagaa ggcgagccuc cauuucucua aggaauccua ugaugaggac cuugagaccc     1020

uguuggguua cauuggcgau gacuauucag auguuuuucu aaaagcaaag aaacuguacg     1080

acgccauccu ccugagcggg uuccuuacgg ugacugacaa ugaaaccgag gcuccucuga     1140

guucugccau gauaaagcgu uauaacgaac acaaagagga uuuggcacuc cugaaggaau     1200

acauucgcaa uaucuccuua aagacauaca acgaggucuu uaaagacgau acuaagaaug     1260

gauaugcugg cuacaucgac gguaaaacca accaggagga uuucuaugug uaccugaaga     1320

aucuacucgc cgaauuugag ggggcggacu auuuccugga aaagauugau agagaggacu     1380

uucuuaggaa gcagcggaca uucgauaacg gaagcauccc auaccagauu cacuugcaag     1440

aaaugcgagc cauacuggac aaacaggcaa aguuuuaucc cuuccucgcu aagaauaaag     1500

agcgcaucga gaagauucug accuucagaa ucccuuacua cgugggccca cuugccaggg     1560

gcaacucgga uuuugcuugg ucuauccgga aacgcaauga aaagauuacg ccguggaacu     1620

ucgaggacgu cauagauaaa gaauccucag ccgaggcauu uaucaauaga augacaaguu     1680

ucgaccugua uuugcccgag gaaaagguuc ucccuaagca uagccuguua uacgagacuu     1740

uuaacgugua uaaugaacug accaaaguaa gguucauugc cgaguccaug cgugacuacc     1800

aguuucuaga uucuaagcag aaaaaggaca ucgugcggcu cuauuucaaa gauaagcgaa     1860

aggucacuga caaagauauu aucgaauacc ugcacgcuau auauggguac gacggaaucg     1920

agcuuaaggg uauugagaaa caauuuaaca gcucauugag uacauaccau gaucugcuca     1980

acaucauuaa ugacaaggag uuccuggaug acuccagcaa cgaagcgauc auagaagaga     2040

uuauccacac cuuaacgauu uuugaagaua gagagaugau caaacagagg cugucuaagu     2100

ucgagaauau auuugacaag ucagugcuua aaaaguugag ucgccggcac uauaccggcu     2160

gggggaaacu cagcgcaaag cugaucaacg gaauuagaga ugaaaaaucc ggcaauacaa     2220

uccuagacua ccugauugac gaugguaucu cuaacaggaa uuucaugcag cucauacaug     2280

acgaugcccu gucguucaag aaaaagauuc aaaaggcuca gaucaucggg gacgaggaua     2340

aaggcaacau uaaggaaguu gugaaaagcc uuccaggauc ccccgccauc aagaaaggca     2400

uuuugcaguc aauaaagauc gucgacgagc uggugaaggu aaugggaggg cgcaaaccug     2460

aaaguauugu uguggagaug gcacgagaga aucaguauac uaaccaaggu aagucuaaua     2520

gccagcagcg guuaaaacgu cucgaaaagu cccugaaaga gcuuggcuca aagauccuga     2580

aggaaaacau uccagccaaa uugagcaaga ucgauaauaa cgcucuccag aaugacagac     2640

uguaccuaua uuaccugcaa aacgggaaag auauguauac cggagacgau cucgacauag     2700

acaggcuguc uaacuacgau aucgaccaca uuauccccca ggccuuucuu aaggauaauu     2760

ccauugacaa caaagucuug gugaguagcg caucuaaucg cggcaagucc gaugacuucc     2820

cgucacugga ggucgugaag aaacggaaga cauuuuggua ccaguuacuc aaaaguaagc     2880

ugaucagcca gagaaaauuc gauaaccuua cuaaggcgga gagggguggg cugcuaccug     2940

aagacaaggc uggcuuuaua caacgacagc ucguugagac ccgccagauu acaaaacaug     3000

uggcccggcu guuggaugaa aaguucaaua acaaaaagga cgagaauaac agagcaguaa     3060

ggaccgucaa aaucaucacu cugaagucga cguuaguguc ccaguuucgu aaagauuucg     3120

aacucuauaa gguucgcgag auuaaugacu uucaccaugc ccacgaugcu uaccugaacg     3180

ccgugauagc aucugcccuu cugaagaaau aucccaaguu ggagccagag uucgucuacg     3240

gagacuaucc uaaauacaau agcuuucggg aaagaaaguc agcuacagaa aaaguguauu     3300

ucuacaguaa caucaugaau auuuucaaga aguccaucuc ucucgcggac ggcaggguaa     3360

uugagcgacc ccugaucgaa gugaacgagg agaccgggga aagcguuugg aauaaagagu     3420

ccgaucuagc cacuguccgg cgcgugcugu cauacccaca agugaacguc guuaagaaag     3480

uggaagaaca gaaccacgga cuugacagag guaagcccaa aggccucuuu aaugcuaacc     3540

ugagcaguaa gccuaagcca aauucuaacg aaaauuuggu gggggcaaaa gaguaucugg     3600

auccgaagaa auacggaggc uaugccggua uauccaacag cuucgcugua uuagucaagg     3660

ggaccaucga gaaaggcgcc aagaagaaaa uuacaaaugu gcucgaauuu cagggaaucu     3720

cgauucugga caggaucaac uaccggaagg auaaacuuaa uuuccuguug gagaagggcu     3780

auaaagacau agaacucauu aucgagcugc ccaaguacuc acuauuugag cugucugaug     3840

ggagucgaag aaugcuugca uccauccuca gcaccaacaa uaaacgcgga gaaauucaua     3900

agggcaacca aaucuuccug ucucagaagu uuguuaaauu guuauaucac gcgaagagga     3960

uaagcaauac uauuaacgag aaucaucgua aauacgugga aaaccacaag aaagaguucg     4020

aggaacuguu uuacuauauc cucgaguuca augaaaacua cgucggugcc aagaagaacg     4080

ggaaacugcu uaauuccgcu uuucagucau ggcagaacca caguauugac gagcugugua     4140

gcucuuucau cggaccuaca ggcuccgaac ggaaggguuu guuugagcua acgucaagag     4200

gcagugccgc agauuucgag uuccuggggg ugaaaauacc aagguaucgc gacuacaccc     4260

ccagcucccu ccugaaggau gcuacucuua uucaucaauc uguuacagga uuauaugaaa     4320

cccgaaucga ccuggccaaa cucggcgagg ggaagagacc ugcagccaca aagaaagcug     4380

gacaggcgaa gaaaaagaaa uaaggacuag uaauaaggaa acuguaugaa ugucuguggg     4440

caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug aauaauaaac     4500

uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   4607


<210>  3606
<211>  3683
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3606
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaug guacgccagc cugauguccg cgcaucaccu caggguugga auugacgugg      180

guacccauuc uguaggccuu gcuacacugc ggguggauga ccacgggacu ccaauagagu      240

uguuaucagc ccugagucau auccacgaua gcggagucgg caaggaaggu aaaaaggacc      300

acgauacgcg caagaaacua uccgggauug cacgacgugc cagaaggcuc cugcaucacc      360

ggcgcaccca gcuucaacag cuggacgagg uguugagaga ucucggauuc ccgaucccca      420

caccuggcga auuucuggac cugaacgagc agacugaucc auauaggguu uggcgggugc      480

gcgcucgacu cgucgaagag aaguuacccg aggaacugag aggcccugca auuucuaugg      540

ccgugaggca uaucgcucgg caccgugggu ggcgcaaucc auacucgaaa guagagucac      600

uuuugagccc cgccgaagag aguccuuuca ugaaggcgcu gagagagagg auacuagcaa      660

ccacaggaga aguucucgac gaugguauca ccccaggcca ggccauggcu caaguggccc      720

ugacucacaa cauuuccaug cgggggccgg agggaauccu uggcaaacug caucagucug      780

acaaugcaaa cgaaauucga aagaucugcg cucgccaggg ugucagcccc gaugugugua      840

agcaguugcu cagagccgug uuuaaagcgg acuccccuag ggggucagcc gucagucggg      900

uugcaccaga cccccugccu ggacaaggca gcuuccgcag agcuccaaag ugcgaucccg      960

aguuucagag guuccguaua auuucuaucg uggccaauuu acggauuucc gaaacaaaag     1020

gcgagaaccg accgcugacc gcugacgaga gaaggcacgu agugacguuu cucacugaag     1080

auucacaggc cgaccuuacc ugggucgaug uggcagagaa gcuggggguu caucgccggg     1140

acuugagagg aacagccgug cacacugaug acggugaaag gagcgcugcg cgcccuccca     1200

ucgaugcaac cgaccgaauc augcggcaga caaaaauuag uucucuaaag accugguggg     1260

aggaagccga uuccgagcaa cguggcgcua ugauaagaua ucuguacgag gacccaacgg     1320

acagcgaaug ugccgagauc auugcagaac ucccugagga agaucaggcc aaacuggacu     1380

cgcuucaucu gcccgcuggg agggccgcau auucucgcga guccuugacu gcgcucucag     1440

aucacaugcu ggcuacaacc gacgauuuac acgaggcccg gaagagacug uucggagucg     1500

acgauaguug ggcaccaccc gccgaagcua ucaaugcccc ugugggcaac ccaagcguag     1560

acaggacucu aaagauaguu ggucgauacc ucuccgcagu ggagucuaug ugggggacac     1620

cggaagucau ucauguggag cacguccgcg auggcuuuac cagcgagcgg auggcugacg     1680

aaagagauaa agccaauagg cgucgcuaua acgacaauca ggaggcgaug aagaaaaucc     1740

aacgggauua cggaaaggaa ggcuacaucu caagagggga cauugugagg cuggacgccc     1800

uugaguugca gggaugcgcu ugucuguauu gcgguacgac caucggcuac cauacauguc     1860

agcucgauca cauuguuccc caggcagggc cuggaaguaa caaucgacgg ggcaaccugg     1920

uggccguaug cgaacgcugu aauagaucca aaagcaacac uccauucgcu gucugggccc     1980

aaaagugcgg uauaccccac guggggguua aggaggcaau cggcagggug cggggauggc     2040

gaaaacagac cccuaacaca ucuucagagg accuuacuag acugaagaaa gaagucauug     2100

cgcgcuugag gcguacccag gaggauccag aaaucgacga gcggaguaug gaaagcgugg     2160

ccuggauggc uaaugagcuc caucacagaa ucgccgcagc uuaucccgag acaaccguaa     2220

ugguguacag gggcuccauu acugccgcag cccgcaaggc ugcggggaua gauucucgaa     2280

ucaaccugau uggagaaaaa gguagaaagg accggaucga uaggcgccau cacgccguug     2340

acgcaucggu cguggcuuua auggaggcca gcguggccaa gacgcuggcu gaacguuccu     2400

cacuaagagg cgagcagcgg cucacaggga aagaacaaac cuggaagcag uauacuggaa     2460

guaccgucgg cgcaagggag cauuuugaga uguggcgcgg ucacaugcug caccuuacag     2520

aauuguucaa ugagcgacug gccgaagaua aaguuuacgu gacccagaac auuagacucc     2580

ggcugucuga cgggaaugcg cauacuguga acccgagcaa guuaguaucc cacaggcugg     2640

gcgauggacu uacaguccag caaaucgacc gcgcuuguac gccugccuug uggugcgcac     2700

ucaccagaga gaaagauuuu gacgagaaga auggccugcc agccagggaa gaccgggcua     2760

uacgugugca ugggcacgag aucaagucaa gcgauuauau ucagguuuuc ucuaaacgca     2820

agaaaacuga cuccgaucga gacgaaacac ccuucggagc aaucgccgug agaggcgguu     2880

uugucgagau ugggccuagu auccaucacg cgaggauaua ccggguggaa ggaaagaaac     2940

ccguuuacgc uaugcuacgc guguucaccc acgaucugcu cagccagaga cauggcgacc     3000

uguuuucugc cguaauucca ccucagucca ucucaaugag gugcgcagag cccaaacuuc     3060

ggaaagccau uacaacuggu aacgcuaccu auuugggcug ggucguggug ggggaugagc     3120

uggaaaucaa ugucgacagu uucacgaagu acgccauagg acgauuuuua gaggauuucc     3180

caaacaccac aagauggcgc aucuguggcu augacacuaa uagcaagcuc acccugaaac     3240

cgauuguucu ugcagcugaa gggcuggaga acccuucguc cgccgugaau gaaaucguag     3300

aguugaaggg auggagggug gcgauuaacg uccucacaaa agugcacccc acuguugugc     3360

ggcgugaugc ccugggcaga ccaagguacu cuagccgcuc aaaucuaccc accaguugga     3420

cgaucgagaa gcggccugca gcuacaaaga aggccgguca agcuaagaaa aagaaauaag     3480

gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag auccuucugu     3540

aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau aaaucuggua     3600

gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaga auu                                             3683


<210>  3607
<211>  4544
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3607
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaac caagccauac agcauuggac uggacauagg uacaaacucc guuggcuggg      180

ccgugacuac ggauaauuau aaaguaccgu cuaagaagau gaaagugcuc gggaacaccu      240

caaagaaaua caucaagaaa aaucuucugg gagucuuguu auucgacagu ggcauuacag      300

cggaggguag gcggcugaag cgcacugcuc gacguagaua uaccaggcgg cgcaacagaa      360

uccuauaccu ccaggaaauu uuuagcacag agauggccac ccuggaugac gcauucuuuc      420

aaaggcuuga ugacuccuuc cuggugcccg augacaagcg ggauucuaaa uauccuaucu      480

uugggaauuu gguugaagag aaggccuacc augacgaguu cccaacuaua uaucaccucc      540

gcaaauaccu ggcugauucg acaaagaaag cagaccugcg acucguguac uuagcccugg      600

cucauaugau caaguauaga ggacacuuuc uuauugaggg cgaguucaac ucaaagaaua      660

acgauaucca gaagaauuuu caggacuucu uggacaccua caacgccauu uuugaaagcg      720

aucugagucu agaaaauucc aagcagcucg aagagaucgu caaagacaag auaucuaaac      780

uggagaagaa agauaggauu cuuaagcugu uccccggcga aaagaacagc gggaucuuuu      840

ccgaguucuu gaaacucauu gugggaaauc aagcggacuu ccggaagugc uuuaaccugg      900

augaaaaagc aucauuacau uucaguaagg agagcuauga cgaagaucug gagacgcucc      960

uugguuacau cggcgacgau uauucugacg uauuucugaa agccaagaag uuguacgaug     1020

cuauccuacu guccggguuc cucacuguua ccgacaauga gacagaagcc ccucugucaa     1080

gcgcaaugau uaaacguuau aacgagcaca aggaagaccu ugcucuguug aaagaguaca     1140

uacgcaacau cagucucaag acuuacaaug aaguguuuaa agaugacacc aagaacggau     1200

augccggcua cauugauggu aagacaaauc aggaggacuu cuaugucuac cugaaaaagu     1260

uacuggcgga guuugaaggg gccgauuauu uccuagagaa aaucgacaga gaagauuuuc     1320

ucaggaagca gcggaccuuc gacaacggau cuauaccaua ccagauucac cuucaagaga     1380

ugcgagcaau ccuugauaaa caggcuaagu uuuaucccuu cuuggccaaa aauaaggaac     1440

gcaucgagaa gauucugacg uuuagaaucc cuuacuacgu gggcccacuc gcuaggggca     1500

acuccgacuu cgccuggagc auucggaaac gcaaugagaa gauaacuccg uggaacuucg     1560

aagaugugau cgacaaagag ucgucugcag aagccuuuau uaauagaaug acauccuucg     1620

aucuguaucu ucccgaggag aagguccugc cuaaacauuc auugcucuac gaaaccuuua     1680

acguuuauaa ugagcugacu aaggugaggu ucaucgcuga aaguaugcgu gacuaccagu     1740

uuuuagacag caagcagaaa aaggauaucg uacggcugua uuucaaagac aagcgaaaag     1800

ugacagauaa ggacauuaua gaguaccuac acgcgaucua uggguacgau ggaauugaac     1860

ucaaggguau cgagaaacaa uuuaacuccu cucugagcac cuaccaugac cuuuugaaua     1920

uuaucaacga uaaggaguuc cuggacgauu caaguaauga agcaauaauc gaggaaauua     1980

uccacacgcu caccauuuuu gaggacagag aaaugaucaa acagaggcug uccaaguucg     2040

agaacauauu ugauaaaagc gucuuaaaga agcugucucg ccggcacuau acaggcuggg     2100

ggaaacuuuc agccaaguug auuaacggaa ucagagacga gaaaaguggc aauacuauuc     2160

ucgauuaccu gaucgacgac gguauaagca acaggaauuu caugcagcua auccaugaug     2220

acgcucuguc cuuuaagaaa aagauucaaa aagcccagau cauuggggau gaagacaagg     2280

gcaacaucaa ggaggugguu aaaucucucc caggaucgcc cgcaauaaag aaaggcauuc     2340

ugcagagcau caagaucgug gaugaacuug ucaaagugau gggagggcgc aagccugagu     2400

ccauuguagu ugaaauggcc cgagagaauc aguauaccaa ccaagguaag ucaaauaguc     2460

agcagcgguu gaaacgucug gagaagucuu uaaaagaacu cggcagcaag auccugaaag     2520

agaacauucc agcuaagcug uccaagauag acaauaacgc ccugcagaau gauagauugu     2580

accucuauua ccugcaaaac gggaaagaca uguauacagg agaugaccua gauaucgaca     2640

ggcugucaaa uuacgacauu gaucacauca uuccccaggc auuccucaag gacaacagca     2700

ucgauaauaa agugcugguc ucuuccgcga guaaccgcgg caagagcgac gaugugccgu     2760

cucuugaagu cgugaaaaag cggaagacuu ucugguacca guugcugaaa uccaaguuaa     2820

uaucacagag aaaauuugac aaccucacca aggcugagag gggugggcug aguccugagg     2880

auaaagccgg cuucauccaa cgacagcuug uugaaacacg ccagauuacc aagcaugugg     2940

cacggcugcu agacgagaag uuuaauaaca aaaaggauga aaauaacaga gccguaagga     3000

cugucaaaau cauuacgcuc aagagcacac uggugucgca guuccguaaa gacuuugagu     3060

uguauaaggu ucgcgaaauc aaugauuucc accaugcuca cgacgccuac cugaacgcag     3120

uggucgcuuc cgccuuacuc aaaaaguauc ccaagcugga gccagaguuu guguacggag     3180

auuauccuaa auacaauucu uuccgggaaa gaaagagcgc gaccgagaaa guauauuuuu     3240

acucaaacau aaugaauauu uucaagaaaa guaucucccu ugccgacggc agggugaucg     3300

aacgaccccu gauugagguu aacgaagaga cuggggaguc ugucuggaau aaggaaagcg     3360

acuuggcuac cgugcggcgc gugcucuccu acccacaagu caacguugug aagaaagugg     3420

aggaacagaa ucacggacug gauagaggua agcccaaagg ccuauuuaac gcaaaucugu     3480

caagcaagcc uaaaccaaac aguaacgaga aucuuguagg ggccaaggaa uaucucgacc     3540

cgaagaaaua cggaggcuau gcugguauau cuaacuccuu cacaguccug gugaagggga     3600

ccaucgagaa aggcgccaag aaaaagauua cuaauguuuu ggaguuccag ggaaucagca     3660

uucuggauag gaucaacuac cggaaggaca aauuaaauuu ucuccuggaa aagggcuaua     3720

aagauauaga gcuuaucauu gaacugccca aguacucguu guucgagcuc ucagacgggu     3780

cucgaagaau gcuggcaagu auccuaucca caaacaauaa acgcggagag auucauaagg     3840

gcaaccaaau cuuucugagc cagaaauucg ugaaacuucu cuaucacgcg aagaggauau     3900

cuaauacgau uaacgaaaau caucguaaau acgucgagaa ccacaagaaa gaauuugagg     3960

aacuguucua cuauaucuug gaguuuaaug agaacuacgu gggugccaag aaaaauggga     4020

aguuacugaa cagcgcuuuc caguccuggc agaaucacuc aaucgaugaa cucuguagua     4080

gcuuuauugg accuaccggc ucugagcgga agggucuguu cgaacuuacu uccagaggcu     4140

cagccgcaga cuuugaguuc cuggggguua aaaucccaag guaucgcgau uacacaccca     4200

guagcuugcu aaaggacgcu acccugauac aucaauccgu gacaggacuc uaugaaacuc     4260

gaauugaucu ggccaaacuu ggcgagggga agagaccugc agccaccaaa aaggcuggac     4320

aggcgaagaa aaagaaauaa ggacuaguaa uaaggaaacu guaugaaugu cugugggcag     4380

gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu     4440

uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4544


<210>  3608
<211>  3626
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3608
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc cgcguucaag ccaaacagca uuaauuacau acugggacuc gacaucggua      180

uugcuuccgu uggcugggcc augguggaga ucgaugaaga ggaaaacccg auuaggcuua      240

ucgaccuggg gguacgggug uuugagcgcg cagaaguccc caaaaccgga gauucuuugg      300

ccauggcucg acguuuagca agaucaguga ggcggcugac acgcagaagg gcccaucggc      360

uacuccgcac ucgaagacug cuuaagaggg agggcguucu gcaggcugcc aauuucgacg      420

agaacgguuu gauaaagagu cucccuaaua cgccauggca acugcgggcg gcagcccugg      480

aucguaaacu cacccccuua gaauggagcg cugugcugcu ucacuugauc aagcaucgcg      540

gguaucuguc ccagaggaaa aacgagggag aaacagccga caaggagcua ggcgcacucc      600

ugaaaggcgu cgcugggaau gcccacgcgc uucagacugg agauuuuagg accccugccg      660

agcuggcauu gaacaaguuc gaaaaggagu cuggucauau ucggaaucag cgaucggacu      720

acucacacac auuuagccgc aaagaucucc aagcugaacu gaucuuacug uucgagaagc      780

agaaagaauu uggcaaccca cacgugagug ggggacucaa ggagggcauu gagacccuuc      840

ugaugacuca gagacccgcc uuguccggug acgcuguaca gaaaaugcua gggcauugca      900

cauucgaacc ugccgagcca aaggcagcca aaaauaccua uacggcugaa agguuuaucu      960

ggcugacuaa gcucaacaau cugcggauac uugagcaagg aucugaacgc ccgcugaccg     1020

auacagagag agcgacuuug auggacgagc ccuacagaaa gagcaaacuc accuaugcac     1080

aggcccguaa gcuguuaggc cuggaagaua cagcuuucuu uaaaggccua cgguacggga     1140

aggacaacgc cgaggcaucc acccucaugg aaaugaaagc cuaucacgcu auuucacgag     1200

cccuggagaa ggaaggacuu aaggacaaaa agaguccuuu gaaucugagc ccagagcucc     1260

aggaugagau cgguacggca uucucucugu uuaaaacuga cgaagauauu acaggcagac     1320

uuaaggacag gauccagccc gagauccugg aagcguugcu caaacauauu uccuucgaua     1380

aguucguuca aauaucacug aaggcuuuac gccggaucgu gccucugaug gagcagggga     1440

aaagauacga cgaggccugu gcagaaauuu acggagauca cuauggcaag aaaaacaccg     1500

aggaaaagau cuaccuacca cccauaccgg ccgacgagau uaggaacccu gucgugcucc     1560

gcgcucugag ccaggcccga aaagugauca auggugucgu ucggcguuau gggagucccg     1620

caagaaucca cauugaaacu gcuagggagg ugggcaaguc uuuuaaggau cgcaaagaga     1680

ucgaaaagcg gcaagaggaa aacagaaaag acagggagaa ggccgcggcc aaauuccgag     1740

aauacuuucc aaauuucgua ggagagccua aguccaaaga uauucuuaag uugcgccugu     1800

augagcagca gcauggcaag ugccucuaca gcgggaaaga aauaaaccug ggacgguuaa     1860

augagaaggg uuauguggaa aucgaccacg cucugcccuu uucgagaaca ugggacgauu     1920

cuuucaacaa uaaaguccuu guguugggcu ccgagaacca gaauaagggg aaccaaaccc     1980

cauacgaaua cuuuaaugga aaagacaacu caagggagug gcaggaguuc aaggcacgug     2040

uugaaacgag ucgcuuuccc cggagcaaga aacagaggau ucuccugcag aaguucgaug     2100

aggacggcuu uaaagaaaga aaucuaaacg auacccgaua ugugaaucgg uuccuguguc     2160

aauuugucgc cgaccgcaug agacucacag guaaggggaa aaagagggug uucgcuucca     2220

acggccagau cacuaaccug cuucggggau ucuggggcuu gcgaaaggua agagccgaga     2280

augaucgcca ucacgcacug gacgcgguug uggucgccug cucuaccgug gcuaugcagc     2340

agaaaaucac aagguuuguc cguuacaagg agaugaacgc cuucgauggg aaaacuauug     2400

acaaggaaac cggagaggug uuacaucaga aaacacacuu uccucagcca ugggaguucu     2460

uugcacagga aguuaugaua cggguguucg guaagccgga uggcaagccc gaauuugagg     2520

aggcugacac ccucgaaaaa cugagaacuc uucuggccga gaaguugagc ucaaggccug     2580

aagcaguaca cgaguauguc acgccacucu ucgugagucg cgcccccaau cgaaaaaugu     2640

ccgggcaggg acauauggaa acaguuaaga gcgcuaaaag acuggaugag ggcgugucug     2700

uccuacgggu gccucugacc caacucaagc ugaaagaccu ugagaagaug guaaacaggg     2760

aacgcgagcc aaaguuguac gaagcgcuga aagcccguuu agaggcacac aaggacgauc     2820

ccgcuaaagc cuuugccgaa ccguucuaua aguacgacaa agcugguaau agaacucaac     2880

aggugaaggc aguucggguc gagcaagugc agaagaccgg gguguggguc aggaaccaua     2940

auggcaucgc cgauaacgcg acaaugguuc gcguggacgu guuugagaaa ggagauaagu     3000

auuaccucgu accuauuuac ucauggcagg ucgcuaaagg cauccugcca gaccgagccg     3060

ugguucaggg gaaggaugaa gaggacuggc aacuuauuga ugacaguuuc aauuuuaaau     3120

ucagccugca ccccaacgau cuaguggaag ucaucaccaa gaaggcaaga auguucggau     3180

auuuugccuc cugucaccgg ggcacuggua auauaaacau caggauucau gaccucgauc     3240

acaaaaucgg gaagaauggc auucuggagg gaaucggugu gaaaacagcu uugucuuucc     3300

agaaguacca gauagacgag cugggcaaag aaauucgccc uugcagauua aagaagaggc     3360

ccccaguucg gaaacguccu gcagccacga agaaagcggg gcaggcuaag aaaaagaagu     3420

aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa gagauccuuc     3480

uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau aauaaaucug     3540

guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa agaauu                                          3626


<210>  3609
<211>  4382
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3609
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaaaccauac accauuggac uggacauagg uacaaacagc guuggcuggg      180

ccgugcucac ugaucaguau gaccuuguaa agaggaagau gaaaaucgcg ggggauuccg      240

agaagaaaca aauuaagaaa aauuucuggg gagugcggcu guuugacgaa ggccagacgg      300

cugccgaucg ccgaauggca cguaccgcca gaaggcggau cgagcgcaga aggaaccgga      360

uuucuuacuu gcaggguauc uucgcugaag agaugucaaa gacagacgca aacuuuuucu      420

gccgcuuaag ugauagcuuu uaugucgaca augaaaagcg aaauuccaga cauccguucu      480

uugccacuau agaggaggaa guggaguacc acaaaaacua ucccaccauc uaccaucuga      540

gggaggagcu aguuaauucu ucggaaaagg cugaucuccg gcugguguau cuugcccugg      600

cgcacauuau caaauaccgu gggaacuucu ugauugaagg agcacucgac acacagaaua      660

ccucagucga uggcaucuac aagcaauuua uacagacuua uaaccaggug uucgccagcg      720

gcauugagga cgggagucug aaaaagcugg aagauaauaa agacguagcu aagauccucg      780

uugagaaggu gacacgcaaa gaaaaguuag agaggauucu gaaacuuuac ccuggagaga      840

aguccgccgg uauguuugca caguucaucu cuuugaucgu cggcagcaaa gggaacuuuc      900

aaaagccauu cgaccugauu gaaaaguccg auauagagug ugcuaaagac ucauaugaag      960

aggaucuaga aagucuccug gcccuuaucg gagacgagua cgcggagcug uucguggccg     1020

caaagaaugc uuauagcgcc guggucuugu cuuccauuau caccguugcu gaaacggaga     1080

cuaacgccaa acucucagca agcaugauag aaagguuuga uacccaugag gaagaccugg     1140

gcgaguuaaa ggccuucauu aaacugcacc uccccaagca cuacgaggaa aucuuuagua     1200

auacagagaa gcaugguuau gcuggguaca ucgauggaaa aacuaagcag gcggacuucu     1260

acaaauauau gaagaugacc cuugaaaaca uugagggcgc agauuacuuu aucgccaaaa     1320

uugagaagga aaacuuccug cggaagcagc gaacauuuga caauggcgcu auaccucacc     1380

aguugcaucu agaggaacug gaggccaucc uccaccaaca ggcaaaauau uacccauucc     1440

ugaaggaaaa cuaugauaaa auuaagucuc uugugaccuu ucgcaucccc uacuucguag     1500

ggccucuggc caauggacag uccgaguuug cuugguugac gagaaaagcc gacggugaga     1560

ucaggccaug gaacauugaa gagaaggugg acuucggcaa aagcgcaguc gauuuuauag     1620

aaaagaugac uaauaaggac acauaucucc cgaaagagaa cgugcugccc aagcacucgu     1680

uaugcuacca aaaauaccug guuuauaaug aacuaaccaa ggugcgguac aucaacgauc     1740

aggggaaaac uucuuauuuc uccggacagg agaaggagca gauuuucaau gaccucuuua     1800

agcaaaaacg caaggucaaa aagaaagauc uggaacuuuu cuugagaaac augucacaug     1860

uggagagucc uacaaucgaa ggccuggagg acagcuuuaa uuccucuuac agcaccuauc     1920

acgaucuccu gaagguaggu auuaagcagg aaauccuuga caacccaguu aauacggaga     1980

ugcuggagaa cauagugaaa aucuugaccg ucuucgaaga uaagaggaug auuaaagagc     2040

agcuccagca auuuucagac gugcuggaug gggucguguu aaagaaacug gaacgucggc     2100

auuacacagg cuggggacga cuaagugcga agcuccugau gggcaucaga gacaagcagu     2160

cccaccuuac uauuuuggau uaucugauga augacgacgg gcucaacagg aaccugaugc     2220

aguuaaucaa ugauagcaac cugucuuuca aaucaauaau ugagaaggag cagguuacca     2280

cagcugacaa agauauccaa aguauugugg ccgaccuugc aggaagcccc gccaucaaga     2340

aagguauauu gcagucccuc aagaucguag augaacuggu cucugugaug ggcuacccuc     2400

cacagacuau uguuguggag auggcucgcg aaaaucagac cacagggaaa ggaaagaaca     2460

auucgcggcc cagauacaag agccuagaga aagccaucaa ggaauuuggc ucccaaauuc     2520

ugaaagagca uccgaccgac aaccaggagc ucaggaauaa ccgccuguau cuuuacuauu     2580

ugcagaaugg uaaggauaug uacacugggc aagaccugga uauccacaac uuaucaaauu     2640

augacauaga ucacauuguc ccucagaguu ucaucacgga caacucuauc gauaaucucg     2700

ugcugacaag cuccgcaggc aaccgagaaa aaggagacga cguaccccca cuugagauug     2760

ugcggaagcg uaaggucuuu ugggaaaaac uguaccaggg caauuugaug ucaaagagaa     2820

aauucgauua ucucaccaag gcugagagag ggggacugac ugaagccgac aaagcgcgcu     2880

uuauccaucg gcagcuaguu gagaccagac aaauuacaaa gaacguggcc aacauacugc     2940

accagagguu caauuacgag aaggaugacc augguaacac caugaaacag gugcgaaucg     3000

ucacucucaa gagcgcucug guuucucagu uucgcaaaca auuccagcuu uacaaggugc     3060

gggaugugaa ugacuaucac cacgcacaug augccuacuu gaacggcgua gucgcuaaua     3120

cacuguuaaa aguguauccu cagcucgaac ccgaguucgu uuacggggac uaucaccagu     3180

uugauugguu caaggccaac aaggcaacgg cgaaaaagca auuuuacacc aauauuaugc     3240

uguucuuugc ccagaaagac agaaucauug augaaaacgg agagauccuu ugggacaaga     3300

aauaucugga cacugugaag aaggucaugu ccuacaggca gaugaauaua gugaaaaaga     3360

cagaaaucca aaaaggcgag uucaguaagg cuaccauuaa accaaagggu aacagcucua     3420

aacuaauccc ccguaagaca aauugggauc cuaugaagua cgggggccuc gacuccccaa     3480

acauggccua ugcaguugug auugaguacg cuaaaggaaa gaauaaacug guauuugaaa     3540

agaaaaucau acgcgucacu auuauggagc ggaaggccuu cgaaaaggau gagaaagcau     3600

uuuuggagga acagggcuau agacagccga aggugcuggc caaauuaccc aaguacaccc     3660

ucuaugagug ugaagagggg aggcgacgga ugcuggcuuc agcgaacgaa gcccagaaag     3720

gaaaucaaca ggugcuuccu aaccaucugg ucacguugcu ccaccaugca gcuaacugcg     3780

agguuaguga cggcaagagc cuggauuaca ucgagucgaa ucgcgaaaug uucgccgagc     3840

uacuggccca cguguccgaa uuugcuaaga gauauacccu ugcagaggcc aaccucaaua     3900

aaaucaacca gcuguucgaa cagaauaagg agggugacau uaaagcgaua gcucaaucuu     3960

ucguagauuu gauggccuuu aacgcaaugg gggccccagc uagcuucaag uuuuucgaga     4020

caacuaucga aaggaaacgg uacaauaacc ugaaggaguu acucaauuca accauuaucu     4080

accagaguau uacaggacug uaugaauccc gaaagagacu ugacgauaaa cgccccgcag     4140

ccacuaagaa agcgggccag gcuaagaaaa agaaauaagg acuaguaaua aggaaacugu     4200

augaaugucu gugggcagga agugaagaga uccuucugua aagguuuuug gaauuauguc     4260

ugcugaauaa uaaacuuuuu ugaaauaaua aaucugguag aaaaaugaga ucuaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     4380

uu                                                                    4382


<210>  3610
<211>  4415
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3610
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaaaccauac agcauuggac uggacauagg uaccaacucc guuggcuggg      180

ccgugguaac agaugacuau aaggugccgg cgaagaaaau gaagguccuc gggaauacug      240

auaaaucuca uaucgagaag aaccuucugg gagcuuuguu auucgacuca ggcaauacgg      300

ccgaagauag gcggcugaaa cgcaccgcac gacguagaua cacaaggcgg cgcaaccgga      360

uucuauaucu ccaggagauc uuuagugaag agauggguaa gguggacgau agcuucuuuc      420

acaggcugga agacuccuuc cuuguuacug aggauaagag aggggagcgc caucccauuu      480

uuggaaaucu ggaagaggaa gugaaauacc acgagaacuu cccuaccauc uaucauuugc      540

gacaauaccu cgccgacaau ccagagaagg ucgaucugag acugguguau cucgcuuuag      600

cacacauaau caaauuuagg ggccacuucc ugauugaagg caaguuugac acacggaaca      660

augauguaca gcgucuuuuc caggaguuuu uggccguuua cgacaacacc uucgaaaauu      720

cuucgcugca ggagcaaaac gugcaggucg aagagauccu aacugacaaa auuucaaaga      780

gcgcuaaaaa ggaucgcgug cucaagcugu uucccaauga gaaaaguaac gggagauucg      840

ccgaguuccu uaagcugauc gugggaaauc aggcggacuu uaaaaagcau uucgaguugg      900

aagagaaagc accucuccag uuuuccaagg auacauacga agaggagcug gaagucuuac      960

uggcccaaau aggugacaac uaugcugaac ucuuccuuuc ugccaagaaa cuguacgaua     1020

gcauuuugcu auccggcauc cugaccguua cggacguggg gacuaaggca ccacucucag     1080

cuaguaugau ucagagguau aacgaacacc agauggaucu ggcccagcuu aaacaauuua     1140

uccggcagaa gcugagcgac aaauacaaug agguauucuc ugaugugucc aaggacggau     1200

augcgggcua caucgauggu aagaccaacc aggaagccuu uuauaaauac uugaaggggc     1260

uccugaauaa aauugaggga ucaggcuacu ucuuagacaa gauagagcga gaagacuuuc     1320

ugcgcaaaca aagaacauuc gauaacggca gcauccccca ucagauucac cuacaggaga     1380

ugagggcaau cauacggcgc caggcugaau uuuauccuuu ccucgccgac aaucaagaua     1440

gaauugagaa gcugcuuacu uuuaggaucc cauacuaugu cgggccguug gcucguggaa     1500

agagugacuu cgccuggcug ucucggaaau ccgcagauaa gaucaccccc uggaacuucg     1560

acgagauugu ggauaaagaa agcucggccg aggcuuuuau caaucgaaug acaaacuacg     1620

accucuaucu gccuaaucag aagguucuuc caaaacacuc ucuguuguac gaaaaguuca     1680

ccguguauaa cgagcucacg aaagucaagu acaagacuga acaggguaaa acagcguuuu     1740

ucgaugcaaa uaugaagcag gagauuuuug acggcguguu caaaguauac agaaagguua     1800

ccaaagauaa gcugauggac uuuuuagaga aggaguucga ugaauuuagg auaguggacc     1860

ugacugggcu agacaaagaa aacaaggucu ucaaugccuc cuauggaaca uaccaugauc     1920

ucugcaaaau ccuggacaag gauuuucuug acaacaguaa aaaugagaag auuuuggaag     1980

auaucgugcu gacccucacg cuguucgagg accgcgagau gauccggaag agauuagaaa     2040

acuauucaga ucugcuuacc aaagagcaag ucaagaaauu ggaaaggcgc cacuacacag     2100

gcuggggucg acucagcgcu gagcugauuc augggauacg gaacaaggaa ucccguaaaa     2160

cuauccuaga cuaucugauu gaugacggca auucuaacag aaauuuuaug cagcucauca     2220

acgaugacgc ccugagcuuc aaggaggaga uugcaaaggc ccaggugauc ggagaaaccg     2280

auaaucuuaa ccagguugug ucagacauag cuggcagucc cgccaucaaa aaggggauuu     2340

ugcaaucccu gaaaaucgua gacgaguuag ucaagauuau gggacaccag ccugaaaaua     2400

ucgugguuga gauggcaagg gagaaccagu ucacaaauca gggucgccgg aacagccaac     2460

agagacucaa aggccugacu gauucuauaa aggaauuugg gucacagauu cuuaaagagc     2520

auccagugga aaauagucaa cugcagaacg acagguuguu ccucuacuau cugcagaaug     2580

gacgagauau guacaccggc gaggaacuag acaucgauua ccugagccag uaugacauug     2640

aucacaucau accccaagcg uuuaucaagg acaacuccau ugauaaucgc guccucacau     2700

cuucgaagga gaaccggggu aaaagcgacg augugccguc caaggacgua gugagaaaaa     2760

ugaagucaua cuggaguaaa cugcuuucug cuaaguugau cacccagagg aaguucgaca     2820

accugacuaa agccgagcgu gggggcuuaa cggaugacga uaaggcagga uuuauuaaac     2880

gccagcucgu ugaaacacgg cagaucacca agcacgucgc cagaauacug gacgagaggu     2940

ucaauacuga aaccgaugag aacaauaaaa agauucgaca agugaagauc gugacacuua     3000

aaagcaaccu ggucuccaau uuucggaagg aguucgaacu auauaaaguu cgcgagauca     3060

acgacuacca ucacgcucau gaugccuauc ucaaugcagu gauuggcaag gcucuguugg     3120

ggguguaccc ucagcuggaa cccgaguuug uauauggaga cuacccacac uuccacgguc     3180

auaaagaaaa caaggccacc gcgaagaaau uuuucuacuc aaauaucaug aacuucuuua     3240

agaaagauga cgucagaacu gauaagaaug gcgagauuau auggaaaaag gacgaacaca     3300

ucagcaacau uaagaaagug uuaucuuauc cucagguuaa uaucgugaag aaagucgagg     3360

agcagacagg gggauucucc aaggaaagua uucuccccaa aggcaacagc gauaagcuga     3420

ucccaaggaa aacgaagaag uuuuacuggg acaccaaaaa guaugguggg uucgacucuc     3480

ccauaguggc cuacuccauc cuuguuauug cugauaucga gaaaggcaag aguaaaaagc     3540

ugaagacugu gaaagcauug guaggaguca caauuaugga aaagaugacc uuugagcggg     3600

acccuguggc cuuccucgag cgaaaaggcu auagaaaugu gcaagaagag aacaucauaa     3660

agcugccaaa auacucacua uuuaagcugg aaaacgggcg caagaggcuu cucgcuagcg     3720

cccgugagcu gcagaaagga aaugaaauug ucuugccgaa ccaucugggc acauuacucu     3780

aucacgcaaa gaauauccau aaaguugaug agcccaagca ccuggacuac guggauaaac     3840

acaaggacga guucaaggaa cuucuggaug uagugucgaa cuuuuccaaa aaguacacuu     3900

uggcggaggg uaaucucgag aaaaucaagg aacuguaugc ccagaacaau ggggaagacc     3960

uaaaagagcu ggcuucuagc uucauuaacc uucucaccuu uacggccaua ggagcuccug     4020

caaccuucaa guuuuucgau aaaaauaucg accggaagag auacacauca acuaccgaga     4080

uucugaacgc cacauugauc caucaaagua uuacuggcuu auaugaaacc aggaucgauc     4140

ugaauaagcu cgguggcgac aaacgcccag cagccacgaa gaaagcuggg caggcgaaga     4200

aaaagaagua aggacuagua auaaggaaac uguaugaaug ucugugggca ggaagugaag     4260

agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua     4320

auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                4415


<210>  3611
<211>  3548
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3611
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaca gacaacaaac cugagcuaca uucucggacu ugaccugggu auagccuccg      180

uuggcugggc ggugguagag aucaaugaaa acgaggaucc aauuggguug aucgacgugg      240

gagucaggau uuucgaacgg gcugaggugc cgaagacugg cgaaucuuua gcccugucac      300

gccgacuagc acguaguacg agaaggcuca uccggcgcag agcccauagg cugcuucugg      360

cuaaacgguu uuugaagcgc gaggguauac ucagcaccau cgaucuggag aaggggcugc      420

ccaaucaagc augggaacuc cgaguugccg gauuagagag aaggcugucc gcuauugaau      480

ggggcgccgu gcuuuugcac cugaucaaac aucggggcua ucuaucuaag cguaaaaacg      540

agucgcagac aaauaacaag gagcucgggg cgcugcuuuc aggagucgca cagaaucacc      600

agcuguugca aagcgacgau uaccgcacuc cugccgaacu cgcucugaaa aaguucgcca      660

aggaggaagg ucauauuaga aaccagaggg gcgcauauac ccacacauuu aaucgguuag      720

accugcucgc ugagcuuaac cuguuguucg cccagcagca ccaauuuggg aauccacauu      780

gcaaagaaca cauccagcag uacaugaccg agcuacugau guggcagaag cccgcgcuca      840

guggagaggc cauacugaaa augcuuggca aguguacuca ugagaaaaac gaguucaagg      900

cagcuaaaca cacauauucc gccgaacgau uuguguggcu gaccaaguug aauaaccucc      960

gcauucugga ggauggugcu gaaagagccu uaaaugagga ggaaaggcaa cugcuaauca     1020

accacccuua cgagaagucu aaacucacgu augcacaggu acggaagcug cuuggguuga     1080

gcgaacaggc cauuuucaaa caucugcgcu acuccaagga gaacgcugaa ucagcgacuu     1140

uuauggagcu caaagcaugg cacgccauca gaaaggcucu ggagaaucaa ggacuuaagg     1200

acaccuggca ggaucuggcc aaaaagccag acuugcucga ugaaaucggc acagcauuca     1260

gucuguacaa aacugacgag gauauucagc aguauuuaac caacaagguu cccaauagcg     1320

ugauaaacgc ccugcuaguc ucucucaauu uugacaaauu caucgaacug ucccuuaagu     1380

cauugaggaa gauucugccu cucauggagc aaggcaaacg uuacgaccag gcuugccggg     1440

agaucuaugg gcaucacuac ggagaagcca accagaagac aagccagcug uuaccagcaa     1500

uaccggcgca agagauucga aaucccgugg ugcugagaac ccuuagucag gcuaggaaag     1560

ucaucaacgc caucauucgc caguaugguu cuccugcacg gguucauauc gaaacgggca     1620

gagaguuggg gaaguccuuu aaagaaaggc gcgagauuca gaagcaacag gaggauaauc     1680

gaacuaagcg ggaaagcgcc gugcagaaau ucaaggaacu cuucucggac uuuucuuccg     1740

aaccaaaauc aaaggauaua cugaaauucc gucuauacga gcagcaacac ggaaaguguc     1800

uguauagugg caaagaaauc aacauucaca gacucaauga gaaggguuac guagagaucg     1860

accaugcucu gcccuuuagc aggacauggg augacuccuu caacaauaag gugcuugucu     1920

uggccucuga aaaccagaau aaagggaacc agaccccuua cgaguggcug caaggcaaga     1980

ucaacagcga acgcuggaaa aauuuugugg cauuaguucu cggaucacag ugcagugcug     2040

ccaagaaaca gcggcugcuu acucagguga uugaugacaa caaguucaua gauagaaauc     2100

ugaacgacac aagguauauc gcgcgauuuu uguccaauua cauucaagag aaccuccugc     2160

uagucggcaa gaauaaaaag aacguguuca ccccaaaugg gcagaucacg gcccugcucc     2220

gcagccggug gggacugauu aaagcuagag aaaacaauaa caggcaccau gcacuugaug     2280

ccaucguagu ugcuugugcc acccccucua ugcagcagaa gauaacacgu uuuauccgcu     2340

ucaaagaggu gcacccguau aagauugaga aucgguacga aauggucgac caagagucag     2400

gugaaaucau uaguccucac uuucccgagc caugggcaua uuucagacag gaggugaaca     2460

ucagggucuu ugacaaucau ccugauacug uguugaagga aaugcugccc gaccgaccac     2520

aggcgaacca ccaguucguu caacccuuau ucgugagccg ggccccuacc cgcaaaaugu     2580

ccggccaggg gcauauggag acaauaaagu cugcuaaaag acucgccgaa ggaauuucgg     2640

uacugaggau cccacuuacu cagcugaagc cgaaccuacu cgagaauaug gucaacaaag     2700

aacgggagcc cgcacuguac gcuggcuuga aggcccgacu ggcagaguuu aaucaagauc     2760

cugccaaggc uuucgcgacc ccauuuuaua aacagggugg gcaacaggug aaggccauua     2820

gaguugaaca ggugcagaaa agcggcgucu uagugcgcga gaacaaugga guagcagaca     2880

acgcuuccau cgugaggaca gauguuuuca uaaagaauaa caaauuuuuc cucgucccca     2940

ucuacaccug gcaaguggcc aagggcauuc ugccuaauaa agccaucgug gcucacaaga     3000

acgaagacga gugggaagag auggaugagg gggcaaaguu uaaauucuca cuuuuuccaa     3060

augaccuggu cgaauugaag acuaaaaagg aguacuucuu uggauauuac auuggucucg     3120

aucgugccac gggcaacauc agucugaaag aacaugacgg ggagauaucu aagggaaagg     3180

auggcguuua ucgggugggu gugaaacuag cgcugagcuu cgaaaaguac cagguagacg     3240

agcuugggaa aaauagacag auuugcaggc cccagcagcg ccagccgguc cgaaagagac     3300

cugcugccac caaaaaggca ggccaagcca agaaaaagaa auaaggacua guaauaagga     3360

aacuguauga augucugugg gcaggaagug aagagauccu ucuguaaagg uuuuuggaau     3420

uaugucugcu gaauaauaaa cuuuuuugaa auaauaaauc ugguagaaaa augagaucua     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaagaauu                                                              3548


<210>  3612
<211>  3626
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3612
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc cgcguucaag ccaaacccga uuaauuacau acugggacuc gacaucggua      180

uugcuagcgu uggcugggcc augguggaga ucgaugaaga cgagaacccc auuugccuua      240

ucgaucuggg gguaagggug uuugaacggg cagagguccc uaaaaccgga gacuccuugg      300

ccauggcucg ccgauuagca cguucuguga gaaggcugac acggcgcaga gcccauaggc      360

uacuccgggc ucgccgacug cuuaagagag aaggcguucu gcaggccgcg gauuucgacg      420

agaaugguuu gauaaaguca cucccaaaca cucccuggca acugagggca gccgcucugg      480

aucggaaacu cacgccuuua gaguggagug ccgugcugcu ucacuugauc aagcaucgug      540

gguaucugag ccagcgcaaa aaugaaggag agaccgcaga caaggaacua ggcgcucucc      600

ugaaaggcgu cgccgauaac gcgcacgccc uucagacagg ggacuuuaga acuccagcag      660

agcuggcuuu gaauaaguuc gagaaggaau ccggacauau uaggaaccag cggggugauu      720

acucucacac cuuuucgcga aaagaccucc aagccgagcu gaucuuacug uucgaaaagc      780

agaaagaguu uggcaauccc cacgugucag ggggacucaa ggaaggcauu gagacacuuc      840

ugaugaccca gcgcccugcu uugagcggug augccguaca gaaaaugcua gggcauugua      900

cuuucgagcc agcagaaccg aaggccgcua aaaacacaua uaccgcggag agauuuaucu      960

ggcugacgaa gcucaauaac cugaggauac uugaacaagg aagugagcgg ccccugacug     1020

acaccgaacg cgcaacauug auggacgagc cuuacagaaa guccaaacuc acuuaugccc     1080

aggcuaggaa gcuguuaggc cuggaggaua ccgccuucuu uaaaggccua cguuacggga     1140

aggacaaugc agaagccucu acacucaugg agaugaaggc uuaucacgcc auuagccggg     1200

cacuggaaaa ggagggacuu aaggauaaaa aguccccauu gaaccuguca cccgaacucc     1260

aggacgagau cgguaccgcg uucagucugu uuaaaacgga ugaggacauu acuggccgac     1320

uuaaggauag aauccagccu gaaauccugg aggcuuugcu caaacauauu agcuucgaca     1380

aguucguuca aauaucucug aaggccuuaa ggcgcaucgu gccacugaug gaacagggga     1440

aacgguacga ugaggcaugc gccgagauuu acggagacca cuauggcaag aaaaauacag     1500

aagagaagau cuaccuaccc ccgauaccug cugaugaaau uagaaacccc gucgugcuca     1560

gggcccuguc ccaggcacgc aaagugauca acggugucgu ucgacgguau gggucaccag     1620

cucguaucca cauugagacc gccagagaag ugggcaagag cuuuaaggac aggaaagaga     1680

ucgagaagcg ccaagaagag aaucggaaag acagagaaaa ggcggccgcu aaauucaggg     1740

aguacuuucc uaacuucgua ggagaaccca agaguaaaga uauucuuaag uugcgacugu     1800

augagcagca gcauggcaag ugucucuacu cugggaaaga gauaaaucug ggacgcuuaa     1860

acgaaaaggg uuauguggag aucgaccacg cacugccauu uucccggacu ugggaugaca     1920

gcuucaauaa caaaguccuu guguugggcu cggaaaauca gaacaagggg aaucaaacac     1980

ccuacgagua cuuuaacgga aaagauaauu cuagagaaug gcaggaguuc aaggccaggg     2040

uugagaccuc ccguuuuccu cgcucaaaga aacagcggau ucuccugcag aaguucgacg     2100

aagauggcuu uaaagagaga aaccuaaaug acacgaggua ugugaaccga uuccugugcc     2160

aauuugucgc ugaucggaug cgccucaccg guaaggggaa aaagagagug uucgccagua     2220

auggccagau cacaaaccug cuuaggggau ucuggggcuu gcggaaggua cgagcagaaa     2280

acgacagaca ucacgcgcug gaugccguug uggucgcuug uagcacugug gccaugcagc     2340

agaaaaucac ccgcuuuguc agguacaagg agaugaaugc auucgacggg aaaacaauug     2400

auaaggagac uggagaagug uuacaucaga aaacccacuu uccacagccg ugggaguucu     2460

uugcucagga aguuaugaua cguguguucg guaagcccga cggcaagccu gaguuugaag     2520

aggccgacac accagagaaa cuccggaccc ugcuugcaga aaagcugucc ucuagacccg     2580

aggccguaca cgaauauguc acuccuuugu ucgugagcag ggcuccaaac cgcaaaaugu     2640

cagggcaggg acauauggag acgguuaaga gugcgaaacg acucgaugaa ggcguguccg     2700

uccugagagu gccccuaaca caacugaagc ucaaagaccu ggagaagaug guaaaucggg     2760

agagggaacc gaaacuuuac gaggccuuga aagcacgccu ggaagcucac aaggaugacc     2820

cugccaaagc cuuugcugag ccauucuaua aguacgauaa agcagguaac cguacccaac     2880

aggugaaggc cguuagaguc gaacaagugc agaagacugg gguguggguc cggaaucaua     2940

acggcaucgc ggacaaugcu accaugguua ggguggaugu guuugagaaa ggagacaagu     3000

auuacuuagu acccauuuac agcuggcagg ucgccaaagg cauccucccu gaucgcgcag     3060

ugguucaggg gaaggacgag gaagauuggc aacugauuga cgauucuuuc aacuuuaaau     3120

ucucacuuca ccccaaugac cugguggagg ucaucacaaa gaaggcccga auguucggau     3180

auuuugcuag uugccacaga ggcaccggua acauaaauau ccggauucau gaccuagauc     3240

acaaaaucgg gaagaacggc auucucgaag gaaucggugu gaaaacugca cugagcuucc     3300

agaaguacca gauagacgag uugggcaaag agauuaggcc augucgccug aagaagagac     3360

cucccguuag gaaacggcca gccgcgacaa agaaagcugg gcaggccaag aaaaagaagu     3420

aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa gagauccuuc     3480

uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau aauaaaucug     3540

guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa agaauu                                          3626


<210>  3613
<211>  3632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3613
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcacccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa guaccacguu ggaauugacg uggguaccuu cagcguaggc cuggccgcga      180

uagaggugga ugacgcuggg augccaauca aaacacucuc ccuugucucu cacauucaug      240

auucaggacu ggacccggau gaaaucaaga gugccgugac uagguuggca agcuccggca      300

uugcccggcg cacgcgacgu uuauauagaa ggaagcggcg cagacugcag caacuagaca      360

aauuuaucca gaggcagggu uggcccguua uagagcucga agauuacucu gacccucugu      420

auccauggaa ggugcgggcu gagcuugcag ccucguacau cgcugaugaa aaagagcgcg      480

gggagaagcu gucagucgcc uugcgacaca uugcgagaca uaggggaugg cggaaccccu      540

augcaaaagu gagcagucuc uaccugccug acggcccauc cgaugccuuc aaggcuaucc      600

gugaagagau uaagcgcgcc ucuggccagc ccguaccuga aaccgcaaca guugggcaaa      660

uggugacucu gugcgagcuc ggaaccuuaa aacugagagg ugagggcggg guccuuagcg      720

cuagguugca gcaguccgac uaugcccggg aaauccagga gauaugucga augcaagaaa      780

uuggacagga gcuguaccgc aagaucauug augugguguu ugcggccgaa ucaccaaaag      840

gcagugcaag cucuagaguc gguaaggacc cgcuacagcc cgggaaaaau agggcucuca      900

aggccuccga cgcuuuccag cgguaccgca ucgccgcacu gaucggaaac cuuagaguua      960

ggguggaugg cgagaaacgu auucugucag uagaggaaaa gaauuuggug uuugaccacc     1020

ucgucaaccu gacaccuaag aaagagccag aaugggugac cauagccgag aucuuaggca     1080

uugaucgggg gcaacugauc ggaacugcua caaugaccga cgauggugaa cgagcgggcg     1140

caagaccccc uacgcacgac acuaauagga gcauaguuaa cagucgcauu gccccacucg     1200

uggauuggug gaagaccgcu ucugcccuug agcagcaugc aauggucaaa gcccugucca     1260

augcugaggu ggacgauuuc gacagccccg aaggggccaa gguacaggca uuuuucgcgg     1320

auuuggacga cgauguucac gcuaaacuag acucgcugca ucucccggug ggacgggccg     1380

cauauucuga ggauacacug gucagacuua cuaggcgcau gcuguccgac ggcguggauu     1440

uguacaccgc ccgacuccaa gaauuuggua ucgagccuuc auggacaccc ccaaccccuc     1500

ggaucgggga acccgucggc aacccagcug uggaccgugu ucugaagacg gugaguagau     1560

gguuagagag cgccacuaag acauggggag cacccgagag gguaauuauc gaacacgucc     1620

gcgagggcuu cgugaccgaa aaacgggcua gagagaugga uggggacaug aggcgacgcg     1680

ccgcgcggaa ugccaagcug uuucaggaga ugcaggaaaa acuaaacguu cagggaaagc     1740

cuuccagagc ugaucucugg agguaucaau cugugcagcg ucagaauugc cagugugcau     1800

acugcgguag cccaauuacu uucucaaaca gugagaugga ccacauaguc ccgcgcgccg     1860

gccaaggguc cacaaauacc cgggaaaacc ugguggcugu augucauaga ugcaaucaga     1920

gcaaaggaaa cacgcccuuu gccaucuggg caaagaacac cucuauugag ggcgugucag     1980

uuaaggaagc ggucgagagg acacgacacu gggugacuga uaccgguaug cggaguacag     2040

acuucaaaaa guucacuaaa gccguggucg agcgcuuuca gagagcuacc auggaugaag     2100

agaucgacgc caggagcaug gaauccguug cauggauggc uaaugagcuu cggucucgag     2160

uggcccagca uuucgcaucg cacgggacaa ccgugagagu auaucgcggc agcuugacug     2220

ccgaagcuag gcgugcgucc ggaaucucag gcaagcugaa auuuuucgac ggggucggaa     2280

agagucggcu cgauagaagg caucacgcca uugacgcagc ugugauagcc uuuacgucug     2340

auuacguugc cgagacacug gcugugcgca gcaaccuuaa gcaaucccag gcacaccgac     2400

aggaggcccc ucaguggaga gaguucaccg guaaagacgc ggaacaucgg gcugccugga     2460

gggucuggug ucaaaagaug gaaaaacugu cagcauugcu cacugaggau cugcgcgacg     2520

aucguguggu ugugaugagc aauguaagau uacggcuggg caacgggucu gcccacaagg     2580

aaaccaucgg aaaacuaucc aaggucaaac ucaguagcca gcugucugug uccgacauug     2640

auaaggcuuc aagugaggca cuuuggugcg ccuugacaag ggagccaggc uuugacccca     2700

aggaaggucu gccugcgaau ccagagcgcc auauccgagu gaacgggacc cacgucuaug     2760

cuggcgauaa uauuggacuc uuccccguua gcgccggcuc gaucgcacug agagggggau     2820

acgccgaauu aggcuccucu uuucaccaug cucgggugua caaaauaacu agcgguaaga     2880

aaccggccuu cgcaaugcug aggguauaua caaucgaccu uuugccuuac cgcaaccagg     2940

aucucuuuuc aguggagcug aagccacaaa cgaugaguau gagacaggcu gagaaaaagc     3000

uaagggacgc ccuggcgacc gggaaugccg aauaucucgg auggcugguc guggacgaug     3060

agcuuguugu ggacacuucc aagauugcaa cagaucaggu caaagcugug gaagccgagu     3120

ugggcaccau ccggcguugg cgcguugacg guuucuuuuc ucccagcaag cugcgauuaa     3180

gaccucucca gauguccaaa gaaggcauua agaaagaguc agcucccgag cugagcaaga     3240

ucauagauag gccagggugg cuuccugcag ugaacaagcu guucagugac ggaaauguca     3300

caguagugcg gcgcgauucu uugggcagag uuaggcucga auccacugcc caucugcccg     3360

ugaccuggaa aguccaaaag cggccagccg cuacgaaaaa ggcggggcag gccaaaaaga     3420

agaaauaagg acuaguaaua aggaaacugu augaaugucu gugggcagga agugaagaga     3480

uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua     3540

aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3632


<210>  3614
<211>  3332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3614
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc caggauucug gcguucgaca uaggaaucag cuccauuggu ugggcuuuuu      180

cugagaacga ugaacucaag gacugcggcg uucggaucuu caccaaagug gagaauccaa      240

agacagggga aucacuugcc cugccgcgcc gauuggcacg uagugccaga aagagguuag      300

cucggcgcaa agcaagacug aaccaucuaa agcaccucau ugccaaugag uuuaaacuga      360

acuacgaaga uuaucagagc uucgacgagu cccuugcuaa ggccuacaaa ggaucucuga      420

ucucgcccua ugaguugagg uuucgggcgc ucaaugaacu gcugucaaag caagauuucg      480

cacgcguaau acuccauauc gccaagcgaa gaggcuacga cgauauuaaa aacagcgacg      540

auaaggagaa aggugcuauc uuaaaggcca uuaaacagaa ugaagagaag cuggcaaacu      600

aucagagugu gggggaguac cuuuauaaag aauacuuuca gaaguucaag gaaaauucca      660

aagaauuuac uaacgucagg aauaagaaag agucuuacga acgguguauc gcucagagcu      720

ucuugaagga cgagcugaaa cuaauauuua agaagcaacg ugaguucgga uuuuccuucu      780

cuaaaaaguu cgaagaggaa gugcucagug uugccuuuua uaaacgcgcg cugaaggauu      840

ucagccaccu ugugggcaac ugcucauuuu ucacggacga gaaaagagcc ccuaaaaauu      900

ccccacuggc auuuauguuc gucgcuuuga ccaggauuau caaccuccug aauaacuuaa      960

agaacacaga aggcauucug uacacuaaag augaccucaa ugcccuucug aacgaggugu     1020

ugaagaaugg gacccuaaca uauaaacaga ccaagaaacu gcucggacug ucagacgauu     1080

acgaguuuaa gggugaaaag ggcacuuauu ucaucgaguu uaaaaaguac aaagaguuca     1140

ucaaggcucu uggggagcau aaccugagcc aggacgauuu gaaugaaauu gccaaagaca     1200

uaacacucau caaggaugag auuaaacuga agaaggcauu agccaaauau gaccugaacc     1260

aaaaucagau cgauagucua ucuaagcucg aguuuaaaga ccaccugaac auauccuuca     1320

aggcucuuaa auugguaacu ccccugaugc ucgaaggaaa gaaguacgau gaggcgugua     1380

augaacugaa ccuuaaaguu gcaauuaaug aggacaagaa agauuuucug ccugccuuca     1440

acgagacgua cuauaaggac gaagugacua auccagucgu guugcgggcu aucaaagagu     1500

accgaaaggu gcucaacgcc cuguuaaaga aauauggcaa gguccacaaa aucaauauug     1560

aacuggcacg cgagguuggu aagaaccaua gccagagagc caaaaucgaa aaggagcaga     1620

acgagaauua caaggcuaaa aaggacgccg aacuagagug cgaaaaacuc gggcugaaga     1680

uuaacucgaa aaauauacuu aaguugaggc uguucaaaga gcaaaaggaa uuuugugcau     1740

auucuggaga gaagaucaaa auuuccgauc uccaggacga gaagaugcug gaaaucgauc     1800

acaucuaccc cuauucacgg aguuucgacg auagcuacau gaacaaagug uuaguauuua     1860

ccaagcagaa ucaagagaaa cugaaccaga caccuuucga agcguuuggc aaugacuccg     1920

cuaaguggca gaagauugag gugcuugcca aaaacuugcc aacuaagaaa cagaagcgca     1980

uacucgauaa aaauuacaag gacaaggaac aaaagaacuu caaggauaga aaucugaacg     2040

acaccaggua uaucgcacgu cuaguccuga auuacacaaa agauuaucuc gacuuucugc     2100

cgcuuucuga ugacgagaac accaaguuga augacacgca gaaaggcagc aaggugcaug     2160

uugaggccaa gucagggaug cugaccagug cuuuacggca cacaugggga uucuccgcca     2220

aagaucgaaa caaccaccuc caucacgcaa uugacgcugu gaucauugcc uacgcgaaua     2280

acagcaucgu caaggccuuu ucugauuuca aaaaggaaca ggagucaaau agugcugaac     2340

uguaugccaa aaagauaagc gagcuugacu acaagaacaa aagaaaguuu uucgagcccu     2400

uuuccgguuu caggcagaaa gugcuggaua agaucgacga aauuuucgua ucuaaaccug     2460

agcgcaagaa accaucgggc gcauugcaug aagagaccuu ucggaaggaa gaggaguucu     2520

aucaaagcua cgggggaaag gaaggcguuc ucaaagcucu ggagcuaggu aagaucagaa     2580

aagugaaugg gaagauuguc aaaaacggcg auauguuuag gguggacauc uucaagcaca     2640

agaaaacuaa uaaguuuuac gccgucccca uauauacaau ggauuucgca cugaaagugc     2700

ucccuaacaa ggcgguugcc cgcuccaaaa agggagaaau uaaggacugg auccugaugg     2760

augagaauua cgaauuuugc uucucacuuu auaaagacag uuugauucug auccagacca     2820

aggauaugca ggagccagag uuuguguacu auaacgcuuu cacgucuagc accguauccu     2880

uaauagucuc aaaacaugac aauaaguuug aaacacucag caaaaaccag aagauccugu     2940

ucaagaaugc caacgagaaa gaagugauug caaagucuau cggcauucaa aaucuuaaag     3000

uuuucgagaa guacaucgug uccgcucugg gggaagucac uaaagccgag uuucgacagc     3060

gggaggacuu caagaaaaag cgucccgcag ccaccaagaa agcuggacag gcgaagaaaa     3120

agaaauaagg acuaguaaua aggaaacugu augaaugucu gugggcagga agugaagaga     3180

uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua     3240

aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3300

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3332


<210>  3615
<211>  3743
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3615
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaag cgaccugguu cucggacuug auauugguau aggcuccgug gggguaggaa      180

uccugaacaa ggugaccggc gagauuaucc auaaaaauuc uaggauuuuc ccagccgcgc      240

aggcugaaaa caauuugguc cggcgcacaa accgacaagg ucguagauua gccaggcgga      300

agaagcaccg cagagugagg cugaaucggc uauuugagga aucagggcuc aucacugacu      360

ucacgaaaau aaguaucaac cugaauccgu accagcuucg cguuaaggga cugaccgaug      420

aguugagcaa cgaagaacuc uuuauugcac ugaaaaauau ggugaagcau cgaggcaucu      480

ccuaucugga cgaugccucu gacgauggca acucgucagu cggggacuac gcucagauug      540

ugaaagagaa uagcaagcag cucgaaacaa agacucccgg acaaauccag uuagagagau      600

aucagaccua cggucagcug aggggcgauu ucacaguaga aaaagacggg aagaaacacc      660

ggcuuauaaa cguuuuuccu accagugcau aucguuccga ggccuugcgc auucugcaaa      720

cucagcagga guucaaucca cagaucacag augaauuuau uaacagauac cuagagaucc      780

ucaccggaaa gaggaaauau uaccauggcc ccgguaauga aaagucucgg acggacuacg      840

ggcgauaucg cacuagcgga gagacccugg auaacaucuu cggcauucuu auaggcaaau      900

gcacauuuua cccugacgag uucagagcug ccaaggcguc cuauacugca caagaguuua      960

accuguugaa ugaccucaac aaucugaccg ugccaacaga gaccaagaaa uuaucaaagg     1020

aacagaaaaa ccagaucauu aauuacguca agaacgagaa agccaugggg cccgcuaagc     1080

uguucaagua uaucgccaaa cuccuuaguu gugauguggc agacauaaag ggauacagga     1140

uugauaaaag cgguaaggcu gaaauccaca cguuugaggc cuaucggaaa augaagacuc     1200

uggaaacauu ggacaucgag caaauggauc gcgagacccu agacaagcug gcguacgugc     1260

ucacucugaa uacagaaaga gagggcauuc aggaagcccu ugagcacgag uucgcagaug     1320

ggucuuucuc ccagaaacag gucgacgagc ugguucaauu uaggaaggcu aacucaagca     1380

ucuucggaaa aggcuggcau aauuuuagug ugaaguugau gauggagcuc auuccugaac     1440

uguacgagac cucugaagaa cagaugacga uauuaacccg ucuggguaaa cagaagacaa     1500

cuuccagcuc gaacaagacc aaauauaucg augagaagcu acucacagaa gagauuuaca     1560

auccaguagu ggccaaaucu guccggcagg cuaucaagau cgugaacgcc gcaauuaaag     1620

aauaugggga cuucgauaau auaguuaucg agauggcccg agaaacuaac gaggacgacg     1680

agaagaaagc uauucaaaag auccagaagg cgaauaaaga ugaaaaggac gcagccaugc     1740

ugaaagcugc caaccaguac aacggcaagg cagagcuucc gcacuccgug uuucauggac     1800

acaaacaguu ggccaccaag auuagacugu ggcaccaaca gggcgaaagg ugccucuaua     1860

cagggaagac caucucaaua caugaucuga ucaauaacag uaaucaguuc gaggucgacc     1920

acauucuucc ccugagcauc acuuuugaug acuccuuggc uaacaaagug cucguauacg     1980

ccacggcaaa ucaggaaaag ggacaacgca caccuuauca ggcgcuggau ucuauggacg     2040

augcuuggag cuuccgggag uuaaaagccu uuguuagaga gucaaagacc cugaguaaca     2100

aaaagaagga auaccuacuc acugaggaag acauuuccaa auucgaugug aggaagaaau     2160

uuaucgagcg caaucugguc gacacccgau acgcaagccg ggugguccuu aacgccuugc     2220

aggaacauuu ccgugcucac aagauagaua caaaaguguc uguugugaga ggucaauuua     2280

ccucacagcu gaggcgccau uggggcauug agaagacucg ggacacauau caccaccaug     2340

ccguagacgc acucaucauu gcugccagua gccagcugaa uuuauggaag aaacagaaga     2400

acacgcuggu cuccuacucu gaggaucaac uuuuggacau cgaaaccggg gagcucauau     2460

cggaugacga auauaaagag agcguguuca aggcgccaua ccagcacuuc guugauacuc     2520

ugaaauccaa ggaguuugaa gacucaaucc uauucaguua ucagguggau ucuaaauuua     2580

auagaaagau uagcgacgcc acaaucuacg cuaccaggca ggcaaagguc ggaaaagaua     2640

aggccgacga gacauaugug cugggcaaaa uuaaggauau cuacacucaa gacgguuacg     2700

augcuuucau gaaaauauau aagaaggaca aauccaaguu ucucauguac cgacaugacc     2760

cccagaccuu cgaaaaagua auugagccua uccuggaaaa cuauccaaau aagcagauca     2820

acgagaaagg gaaggaggug cccuguaauc cguuucuuaa guacaaagag gaacacggcu     2880

auauucgcaa guacagcaaa aagggaaacg gcccugaaau caaaucauug aaguauuacg     2940

auucuaagcu ggggaaccac auugacauaa cgcccaaaga uuccaauaac aagguugucu     3000

uacagagugu gagcccaugg cgggccgacg uguacuucaa uaaaaccaca ggaaaguaug     3060

agauccucgg ucugaaauac gcagaucuuc aauuugaaaa gggcacuggg accuauaaga     3120

uuucucagga gaaauacaac gacaucaaga aaaaggaggg agucgauucc gacucagagu     3180

ucaaauuuac acuguauaag aaugauuugc uccugguuaa agacacugaa accaaggaac     3240

agcaacuauu cagauuucug aguaggacga ugccuaagca gaaacauuac guggagcuca     3300

agcccuauga uaaacagaag uucgaaggcg gugaggcgcu gauuaaagug cuugggaacg     3360

uagccaauag cggccagugc aagaagggau ugggcaaauc gaacaucucc auauacaagg     3420

uccguacaga cgugcugggg aaucaacaca ucauuaaaaa cgagggagac aagccaaaau     3480

uagauuucaa gcgccccgcu gccaccaaga aagcaggcca ggcuaagaaa aagaaauaag     3540

gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag auccuucugu     3600

aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau aaaucuggua     3660

gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaga auu                                             3743


<210>  3616
<211>  4301
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3616
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaac ccaguucgag ggauuuacaa accuguacca aguuagcaag acucucaggu      180

ucgaacuuau uccacagggu aaaacgcuga agcauauaca ggagcagggc uuuaucgaag      240

aggacaaggc ccggaaugau cacuauaaag aauugaagcc gauuaucgac cgcauuuaca      300

aaaccuaugc ggaucaaugc uuacagcugg ugcagcuaga cugggagaac cucuccgcug      360

ccaucgauuc uuaccgaaag gagaaaacag aagagacucg uaaugcacug auagaggaac      420

aggccaccua uagaaacgcu auccaugacu acuucauugg gaggacagau aaucuuaccg      480

acgcaaucaa caagcggcac gccgagauuu auaagggacu guuuaaagcu gaauuguuca      540

auggcaaggu acucaaacaa cuggguacug ugacaaccac ggagcaugaa aacgcccugc      600

uccgcucauu ugauaaguuc acuaccuacu uuaguggguu cuacgagaau aggaaaaacg      660

ucuuuagcgc ggaagacauc uccacagcaa uaccccacag aauugugcag gauaauuucc      720

cuaaguuuaa agagaacugu cacaucuuca cucgguuaau uaccgccguu ccaucucugc      780

gcgagcauuu cgaaaaugug aagaaggcua ucggaaucuu ugucucgaca ucaauugagg      840

aaguguucag cuuucccuuc uauaaccagc uuuugaccca gacgcaaaua gaccuguaca      900

aucagcuacu cggcggcauc agucgagagg ccgggacuga gaaaauuaag ggacugaacg      960

aaguacuuaa ccuggcaauc cagaaaaaug augagacagc ucacauaauu gccuccuugc     1020

cucauagauu uaucccacuc uucaagcaaa uccugucuga caggaacacc cuuagcuuua     1080

uucuggaaga guucaaaucc gacgaagagg uuauccaguc auuuugcaag uauaagacuc     1140

uccuucggaa ugaaaacgug cuggagacag cggaggccuu guucaaugaa cuaaacagua     1200

uugaucugac ccacauauuu aucagccaca aaaagcucga gacgauuucu uccgcacugu     1260

gugaccauug ggauacccuu cguaaugcuc uguacgaacg cagaaucuca gaguugacag     1320

guaaaaucac uaagagcgcc aaagagaagg uccagaggag ucucaagcac gaagacauua     1380

accugcagga gauaaucucu gcugccggca aagaauuauc cgaggcauuc aagcagaaaa     1440

ccagcgaaau ucugucgcau gcccacgcug cgcuagauca accccucccu acaacucuga     1500

agaaacagga ggagaaggaa auccuuaagu cucaguugga cucccugcuc gggcuguauc     1560

aucuucugga uugguuugca guggacgagu caaaugaagu ggauccagag uucagugccc     1620

gguugaccgg aauuaaacuc gaaauggagc cgagccuguc cuucuacaac aaggcucgaa     1680

auuaugccac uaaaaagccc uacucugucg agaaauuuaa guuaaacuuc caaaugccua     1740

cccuggcaag cggcugggac guuaauaaag aaaagaacaa uggugccauc cuauuuguga     1800

agaacgggcu cuacuaucug ggaauaaugc caaaacagaa gggccgcuac aaagcucuuu     1860

cauucgagcc cacugaaaag acgagugagg gcuuugauaa aauguauuac gacuauuucc     1920

cugacgccgc aaagaugauc ccaaagugcu ccacacaguu gaaagcggua accgcucacu     1980

uucagacuca caccacaccc auucugcuca gcaacaauuu caucgaaccg cuggagauua     2040

ccaaggagau cuacgauuua aacaauccug aaaaagagcc caagaaauuu caaacugccu     2100

augcaaagaa gacaggggac cagaaaggau acagagaagc ccuguguaag uggauagauu     2160

ucacgaggga cuuucuuucu aaauacacca agacuacauc aauugauuug aguagccucc     2220

ggccauccuc ucaguauaaa gaccugggug aguacuaugc ugagcuaaac ccucugcucu     2280

accauaucuc guuccagcgc auugccgaaa aggagaucau ggaugcagug gaaaccggca     2340

agcuguaucu uuuucaaaua uacaauaaag acuucgcuaa ggggcaccau ggaaaaccca     2400

acuugcacac acuguauugg acuggcuuau ucagcccaga gaaucucgcc aagaccucca     2460

ucaaacugaa cggucaggcg gaauuauuuu acagacccaa gucaaggaug aaacguaugg     2520

cccaccggcu gggggagaag auguugaaua agaaacucaa ggaucagaaa acgccuauuc     2580

cagacacccu guaccaagag cuauaugauu acgucaacca ucgacugagu cacgaccucu     2640

cugaugaagc uagagcacug cuuccgaaug ugaucacaaa ggagguuagc caugaaauua     2700

ucaaagacag gcgcuucacu uccgacaagu uuuucuuuca cgugcccaua accuugaacu     2760

aucaggccgc uaauucaccu agcaaguuca accagcgggu caaugccuac cugaaagagc     2820

auccagaaac acccauuauc ggcaucgaua gaggagagag gaacuuaauu uauaucacug     2880

ugauugacuc uaccggcaag auacucgagc agcgcucccu gaacacgauc caacaguuug     2940

auuaccagaa aaagcuggac aaucgagaaa aagagcgggu agcagcgcgu caggccugga     3000

guguuguggg gacaauuaag gaucuuaagc aaggauaucu aagccagguc auccacgaaa     3060

uuguggaccu caugauccac uaccaggcug ucgugguucu ggagaacuug aauuucgguu     3120

uuaaaucuaa gagaaccggc auagccgaaa aagcagugua ccagcaauuc gagaagaugc     3180

ugaucgauaa auuaaacugc cucguacuga aggacuaucc ugcugagaag gucgggggag     3240

ugcuuaaucc auaccagcug acugaucagu uuacauccuu cgccaaaaug ggcacccaau     3300

cagguuucuu guuuuauguu cccgcaccgu acacaaguaa gauugacccu cucaccgggu     3360

ucguggaucc auuugucugg aaaacaauca agaaccauga aagcaggaaa cacuuccugg     3420

agggcuuuga cuuccuacau uaugauguga agacgggaga cuuuauucug cacuucaaga     3480

ugaaucgcaa ccuuucguuu cagcggggcc uccccggguu caugccugcc ugggacaucg     3540

uauuugagaa aaaugaaacc caguucgaug cuaagggaac ucccuuuaua gcggguaaaa     3600

gaauugugcc aguuaucgag aaccauaggu ucacaggccg auaccgcgac cuguauccug     3660

ccaaugaauu gaucgcacug uuagaggaaa aggggauugu cuuccgggau ggauccaaca     3720

uacuccccaa acugcuugag aaugacgauu cucacgcuau cgacaccaug guggcccuga     3780

uuagaagcgu guugcagaug aggaacucaa augccgcuac aggcgaagau uacaucaaca     3840

guccaguccg ugaccucaac gguguuuguu uugauucccg cuuccaaaau ccggaguggc     3900

cuauggacgc agaugccaac ggggcguacc acauugcucu gaagggccag cuacugcuua     3960

aucaucucaa agagucuaag gaccugaagu ugcagaacgg aaucagcaau caggacuggu     4020

uagccuauau acaagaacug cggaacaaaa gacccgcagc cacuaagaaa gcuggccagg     4080

caaagaaaaa gaaguaagga cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa     4140

gugaagagau ccuucuguaa agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu     4200

gaaauaauaa aucugguaga aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         4301


<210>  3617
<211>  4280
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3617
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaag cauuuaccag gaguucguua acaaguauuc ccugucuaaa acccucaggu      180

uugaacuuau accacaagga aagacacugg agaauaucaa ggcccggggu uugauuuuag      240

acgaugaaaa acgcgcgaag gacuacaaaa aggcuaaaca gaucauugau aaguaucauc      300

aguucuuuau cgaggaaaua cugucaagug ugugcaucag cgaggaccua cuccagaacu      360

acuccgaugu auauuucaag cugaaaaagu cugacgauga caaucuucaa aaagauuuua      420

agucggccaa agacacuauu aagaaacaga ucucagagua cauuaaggau agcgaaaagu      480

ucaaaaaccu guuuaaucag aacuugaucg acgcaaagaa aggccaggag agugaucuca      540

uacuguggcu gaagcaaucc aaagacaaug ggauugaacu cuucaaggcc aacucugaca      600

ucacggauau ugacgaggcu uuagagauca ucaagagcuu uaaaggaugg accacauauu      660

ucaagggcuu ucacgaaaau cguaaaaacg uguacuccuc aaaugauauu ccgacaagua      720

uaaucuaccg aauugucgac gauaaccugc ccaaguuccu ugagaauaaa gcaaaguaug      780

agagcuugaa ggacaaagcc ccugaggcua ucaacuacga acagauaaag aaagaucugg      840

ccgaggagcu aaccuuugac auugauuaua agacaucuga agugaaucag agaguuuucu      900

cccucgacga gguguucgaa aucgcgaacu uuaacaauua ccugaaccag ucagguauca      960

ccaaauucaa uacuauuauc gggggaaagu uugucaacgg cgagaauaca aagaggaaag     1020

gcauuaacga auauauaaau cuuuacagcc aacagaucaa cgauaagacc cugaaaaagu     1080

auaaaaugag uguguuguuc aagcagauuc ucucugacac ggaguccaag agcuuuguaa     1140

ucgacaaacu ggaggaugac ucggauguug ugacuaccau gcaaucuuuc uacgaacaga     1200

ucgcagccuu uaagacaguc gaggaaaaau ccauuaagga gacuuuauca cuccuguucg     1260

acgaucuuaa agcucagaag cuggacuuga guaaaauaua cuuuaagaau gauaagagcc     1320

uaaccgaccu gucccagcaa guguucgaug acuauucugu gaucgggaca gccguccucg     1380

aauacauuac ccagcagauc gcacccaaaa accuggauaa uccaagcaag aaagagcagg     1440

agcuuauugc uaagaaaacg gaaaaggcca aguaucuguc auuggagacu aucaaacucg     1500

cgcuggaaga guuuaacaag caucgggaca uagauaaaca augucgcuuc gaggaaaucu     1560

uagccaauuu ugcagcuauu ccuaugaucu ucgacgagau ugcccagaac aaggacaauc     1620

uggcucagau caguauaaaa uaccagaacc aaggaaagaa ggaucuacuc caggccuccg     1680

cagaagacga uguuaaagcc auuaaggacc ugcuugauca gacaaacaau uugcugcaca     1740

aacucaagau cuuccauauu agccagucug aggacaaagc uaacauccug gauaaggacg     1800

aacacuuuua ucuuguguuc gaggagugcu acuuugaacu ggcgaauaua guaccauugu     1860

auaacaaaau cagaaauuac auuacccaaa agcccuacuc agaugagaag uucaaacuca     1920

acuuugaaaa uaguacucug gcaaacgguu gggacaagaa uaaagagccu gauaacacag     1980

ccaucuuauu cauuaaggac gauaaauauu accugggcgu gaugaauaag aagaacaaua     2040

aaaucuuuga cgacaaggcu auaaaagaaa acaaggggga gggauauaaa aagauugucu     2100

acaagcuacu cccaggcgcc aauaaaaugc ugccgaaggu guucuuuagc gcaaaaucca     2160

ucaaguucua uaaccccucu gaggauaucc uuaggauucg gaaccacucg acccauacga     2220

aaaaugguag cccucagaag ggguacgaaa aguuugaguu caacaucgaa gacugucgca     2280

aauuuauuga uuucuauaag caguccauau caaaacaccc agaguggaag gacuucggau     2340

uucgauucag ugauacccaa agauacaauu cuaucgacga auuuuacagg gagguugaga     2400

accagggcua uaaauugaca uucgaaaaua uuagcgaguc cuacaucgau ucagugguca     2460

accagggcaa gcuguaucuc uuucagauuu acaauaagga cuucagcgcc uauucuaaag     2520

ggcggcccaa ccugcauacu uuauacugga aggcucuguu ugaugaacgu aaucuucaag     2580

acgugguaua uaaauugaac ggagaggccg aacucuucua ccgcaagcag uccaucccua     2640

aaaagauaac ccacccagca aaagaagcga ucgcuaauaa gaacaaggau aaucccaaaa     2700

aggagagugu uuuugaauac gaccugauua aagacaagag auucacagag gauaaauuuu     2760

ucuuccacug cccgaucacu auuaacuuua agagcucugg ugccaauaag uucaacgacg     2820

aaaucaaccu acugcucaaa gagaaggcaa augaugugca uauacugucc auugacaggg     2880

gcgagcggca ccuugccuau uacaccuugg ucgaugggaa aggaaacauc aucaagcagg     2940

acacauuuaa uauuauaggc aacgaucgaa ugaaaaccaa uuaucaugac aagcuggcug     3000

ccaucgaaaa ggaucgcgac ucagcaagaa aagauuggaa gaaaauuaac aauaucaagg     3060

agaugaaaga agguuacuua agucaggugg uccacgagau ugcuaagcuc gugaucgaau     3120

auaacgccau aguuguguuc gaggaccuga auuuuggguu caagaggggc cgguuuaaag     3180

uagagaagca agucuaccag aaacuugaaa agaugcugau cgagaaauug aacuaucucg     3240

uguucaagga uaaugaauuu gacaaaacug gaggcguucu gcgcgcguac cagcuaacgg     3300

ccccuuucga gacauuuaag aagaugggga aacagaccgg aauuaucuac uaugugcccg     3360

cugguuucac uagcaagauu uguccaguca ccggcuuugu gaaccaacug uacccuaaau     3420

augaaucggu auccaagucu caggaguucu ucagcaaauu ugacaagauc ugcuacaauc     3480

ucgauaaggg guauuucgag uuuucauucg acuacaaaaa cuuuggagau aaggcagcca     3540

aaggcaagug gacaauagcu aguuucgguu ccagacugau uaauuuuagg aacucugaca     3600

aaaaucauaa cugggauacc cgugaagugu aucccacuaa ggagcuugaa aaguugcuga     3660

aagacuacag caucgaguac gggcacggcg aguguaucaa ggccgcaauu ugcggagaau     3720

ccgauaaaaa guucuuugcg aaauuaacau caguucucaa cacgauccug cagaugcgga     3780

auagcaagac cggcacugag cuugacuauc ugauaagucc agucgccgau gugaacggga     3840

auuucuuuga cucucgacaa gcucccaaga acaugccuca ggaugccgac gcaaauggag     3900

cuuaccacau uggucuaaaa ggccucaugc uguuggggag aaucaagaac aaucaggaag     3960

gcaaaaagcu gaacuuagug auuaaaaaug aggaauauuu cgaguuuguc cagaacagga     4020

auaacaagcg cccagccgca acuaaaaagg ccggacaagc uaagaaaaag aaauaaggac     4080

uaguaauaag gaaacuguau gaaugucugu gggcaggaag ugaagagauc cuucuguaaa     4140

gguuuuugga auuaugucug cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa     4200

aaaugagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaagaauu                                                 4280


<210>  3618
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3618
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagacaa gaaauacagc      240

auuggacugg auauagguac aaacuccguu ggcugggccg ugaucacaga cgaguauaag      300

guaccaucua agaaauucaa ggugcucggg aauacugaua ggcauucaau uaaaaagaac      360

cuuaucggag cgcuguuguu ugacaguggc gaaacggcug aggccacccg guuaaaacgc      420

acagcacgac guagauacac uaggcggaag aaucgcauuu gcuaucugca ggaaaucuuc      480

agcaacgaga uggccaaggu cgaugacucc uuuuuccaca gacuagaaga gucuuuucuc      540

guggaggaag auaaaaagca ugagaggcac ccgauauucg guaauaucgu ugacgaagug      600

gcuuaccaug agaaauaucc caccauuuac caucugcgga agaaacuugu cgauucgaca      660

gacaaggcag aucugcgcuu gaucuaucuc gcccuggcuc acaugauuaa auuucgaggg      720

cacuuccuga ucgagggaga ccucaacccu gauaauucag acguggacaa guuauuuaua      780

caacugguac agaccuacaa ccagcuuuuc gaagagaauc caauuaacgc cagcggcguu      840

gaugcgaagg caaucuugag ugccagacug uccaaaucua ggcggcuaga aaaucucauu      900

gcucagcugc ccggcgagaa gaaaaacggg cuuuuuggaa aucugaucgc cuugagccuc      960

ggucugacuc cuaacuucaa guccaauuuu gacuuagcag aagaugcuaa acugcaacuc     1020

ucaaaggaca cauacgauga cgaucuugac aaccuguugg cccagaucgg cgaucaguau     1080

gcggaccuau uccuggccgc aaagaaccuc agugaugcua uucugcuuag cgacauacug     1140

cgugugaaua ccgagaucac gaaagcccca uugucugcuu ccaugauuaa gcgcuacgac     1200

gagcaccauc aggaucucac ucuguuaaaa gcccugguca gacaacagcu acccgaaaag     1260

uauaaagaga ucuucuuuga ccagucaaag aacggguacg caggauauau agauggcggu     1320

gccagccagg aagaguucua caaguuuauu aaaccuaucc ucgaaaagau ggacgggacc     1380

gaggagcugc uugugaaauu gaauagggaa gaucugcucc ggaagcaacg aacauucgac     1440

aacggaagua ucccacacca gauucaccug ggcgagcuuc augcuauccu gcgcagacag     1500

gaagauuuuu auccguucuu gaaagacaau agggagaaga uugaaaagau acucacuuuu     1560

cggauccccu acuacguggg cccucuggcg cgcgggaacu cuagauucgc auggaugacc     1620

aggaaauccg aggagacaau uaccccaugg aauuuugaag aggucguuga uaagggagcc     1680

agcgcucaau cguucaucga acguaugacg aacuuugaca aaaauuuacc caacgagaag     1740

gugcugccua aacacucucu acucuaugag uacuucacug uauauaauga acugacaaag     1800

gugaaauacg ucaccgaggg uaugcggaag ccagccuuuc uuuccggcga acagaagaaa     1860

gcaaucgugg auuugcuguu caagacuaac cgaaaaguua cagugaagca gcucaaagag     1920

gacuauuuca agaagauuga auguuuugau ucagucgaga uaaguggggu ggaggacaga     1980

uucaaugcca gccugggaac cuaccaugac uuacugaaaa ucauuaagga uaaagacuuu     2040

cuugauaacg aagagaauga agacaucuug gaggauauug uacucacgcu gacccuauuc     2100

gaagacaggg agaugaucga ggaacgccug aagacauaug cucaccucuu ugaugacaaa     2160

guuaugaagc agcugaagcg gagaagguac acuggcuggg gucgccuuuc ccgaaaauug     2220

auaaacggga uccgggauaa gcaaucuggc aaaaccauuc uggacuucuu aaagagcgau     2280

ggauuugcca accguaauuu caugcagcuc auccaugacg auucacugac auuuaaagag     2340

gacauucaga aggcacaggu gaguggccaa ggggacuccc uucacgaaca caucgcgaac     2400

cuggcuggaa gccccgccau aaagaaaggu auuuugcaga cugucaaggu ggucgaugag     2460

cucgugaaag uuaugggcag acauaagccg gaaaauaucg ugauugagau ggcaagggag     2520

aaccagacca cacagaaagg gcaaaagaac ucucgcgaac ggaugaaaag aaucgaggaa     2580

ggaauaaagg agcugggcuc acagauccua aaggagcacc cuguagaaaa uacccagcug     2640

cagaaugaga aacucuaccu guauuaccuu caaaacggua gggacaugua ugucgaucag     2700

gaauuggaca uuaaucgacu gagugauuac gacguggauc auaucguucc ccagagcuuc     2760

uuaaaggacg auuccauuga caacaaagug cucacucgcu cugauaagaa ucgggggaaa     2820

ucggacaacg ucccaagcga ggaaguggua aagaagauga aaaauuauug gagacaacug     2880

cuuaacgcca agcugaucac gcagaggaaa uuugacaauc uaacaaaggc ugagcguggc     2940

ggacucuccg agcuggauaa agccggcuuc auaaagcgcc aguuggugga aacccggcag     3000

auuacuaagc acguugcaca aauccuggac ucaagaauga acaccaaaua cgaugagaac     3060

gacaaguuaa ucagggaagu caaagugauu acacucaaga guaaacuggu gucugauuuu     3120

cgaaaggacu uccaguucua uaagguccgg gagaucaaua acuaccacca ugcucacgau     3180

gccuaccuua augcgguugu ggggaccgcc cugauuaaaa aguauccuaa auuggaaagc     3240

gaguuugugu acggagacua uaagguauac gauguccgca aaaugauagc uaaguccgag     3300

caggaaaucg guaaggcaac ugccaaauau uucuuuuacu caaacauuau gaauuucuuu     3360

aagacagaga ucacgcucgc uaacggcgaa auuagaaaaa ggccccugau cgagaccaau     3420

ggggaaacug gagagauagu gugggacaag ggccgggauu ucgccacagu ucgaaaagug     3480

cuaagcaugc cacaggucaa caucgugaag aaaaccgagg uucaaacagg uggguuuucu     3540

aaggaaucca uucugcccaa gagaaauagu gacaaacuua ucgcacgcaa gaaagauugg     3600

gacccuaaga aauauggcgg auucgacagc ccaacugugg cguacucugu acucgucgug     3660

gccaaggugg agaagggcaa auccaagaaa cugaagucag ucaaagaauu gcuggggauu     3720

accaucaugg agaggaguag cuuugagaag aacccgauag auuucuuaga agcuaaggga     3780

uacaaagagg uuaagaaaga ccucauuauc aagcugccca aauauucgcu uuuugaacug     3840

gagaauggcc guaagcggau guuggccucc gcuggugaac uccagaaggg gaacgagcug     3900

gcacuaccuu cuaaauacgu gaauuuccug uaucuugcca gccauuacga gaagcucaaa     3960

ggaucaccag aagauaacga gcagaagcag cuguucguag aacaacacaa acauuauuug     4020

gacgagauca uugaacagau aagugaguuu uccaagagag ugaucuuagc agaugccaau     4080

cuggacaaag uccucucugc uuacaacaag cacagggaua agcccauucg cgagcaggcg     4140

gaaaacauca uucaccuguu cacgcuuacc aaucugggcg ccccugcagc uuuuaaauau     4200

uucgacacaa cuaucgaucg aaagagauac accagcacaa aagagguguu ggacgccacu     4260

cuaauacauc aauccaucac cggucuguac gaaacgcgga uugaucucuc acagcugggc     4320

ggggacaaga ggccagccgc uaccaaaaag gcaggacagg ccaagaaaaa gaaauaagga     4380

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     4440

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     4500

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaagaau u                                               4581


<210>  3619
<211>  5364
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3619
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaacuu caagauucug      240

ccaauagcca ucgaccucgg aguuaaaaau accggugugu uuagcgcguu cuaccagaag      300

ggcacauccc uugagaggcu ggauaacaag aaugggaaag uauaugaauu gucuaaggac      360

ucauacacuu uacugaugaa caaucggacg gcucgccgac aucaacguag aggaauugau      420

aggaaacagc uagugaagcg gcucuuuaaa cugaucugga ccgagcagcu uaaccuggaa      480

ugggacaagg auacacagca agccauuagu uucuuguuua aucgcagagg cuucagcuuu      540

aucacugacg guuauucccc ggaguaccuc aacauagucc ccgaacaggu gaaggcaauc      600

cugauggaua uuuucgacga uuauaauggg gaggacgauc uggacucuua ccucaaauua      660

gccaccgagc aggaaucgaa gaucucagag auuuauaaca aacugaugca gaagauccuu      720

gaauuuaaau ugaugaagcu gugcacagau auaaaagacg acaagguuag caccaagacu      780

cuaaaagaga uuacaaguua cgaguucgaa cuccuggcug auuaccuugc aaauuauucc      840

gagucucuga agacccaaaa auuuagcuac acggacaagc agggaaacuu gaaagaacuc      900

uccuauuacc accaugauaa guauaauauc caggaguucc ugaagaggca cgccacuauu      960

aacgaccgga ucuuagauac ccugcucaca gacgaucuug acaucuggaa uuuuaacuuc     1020

gaaaaauucg auuuugacaa gaacgaggag aaacugcaga aucaagaaga uaaggaccau     1080

auucaggcuc acuugcacca uuucguguuu gccgucaaca aaauaaaguc agagauggcg     1140

aguggcggcc gccaccgaag ccaguacuuc caagaaauca cuaaugugcu agacgagaac     1200

aaucaucagg aaggguaucu gaagaacuuu ugugagaauc uccacaacaa aaaguacucu     1260

aaucuguccg uuaaaaaccu uguaaaucug auuggaaacu ugucaaaucu cgagcugaag     1320

ccuuuaagaa aauacuucaa cgauaagauc cacgcaaagg ccgaccauug ggaugaacag     1380

aaauuuaccg agacauauug ccacuggaua cugggugaau ggaggguggg cgucaaggac     1440

caggauaaaa aggacggggc uaaauacagc uauaaggauc uauguaauga gcucaaacaa     1500

aaggugacca aggccggacu gguggacuuc cuuuuggaac uggauccaug ccggacgauu     1560

cccccuuacc ucgacaacaa caaucguaaa ccacccaagu gucagagucu gauccuuaac     1620

ccuaaauuuc uggauaauca guauccaaac uggcagcaau acuugcagga gcucaagaaa     1680

cugcagucua uccagaauua uuuagacucc uucgagacug aucugaaggu ccuaaagagc     1740

ucgaaagacc aaccguacuu uguugaauac aagucuucca accagcagau ugcaucaggc     1800

cagcgcgacu auaaagaucu cgacgcuaga auccugcaau ucauuuuuga uagggugaag     1860

gccagugacg agcuuuugcu gaaugaaaua uacuuccagg cgaaaaagcu caagcagaaa     1920

gccagcuccg agcuggagaa guuagaaucu agcaaaaagc uggaugaggu aaucgcaaac     1980

ucacaacuua gucagauuuu gaaaucccag cauacaaaug guaucuucga acaggggacc     2040

uuucuccacc uggugugcaa guauuacaag caacggcagc gagcucgcga cagcagacua     2100

uauaucaugc ccgaguacag guaugauaaa aagcugcaua aauacaacaa uacuggacgg     2160

uucgacgaug acaaccagcu ccugacauac uguaaucaca agccucgcca gaaaagauau     2220

caacuuuuga acgaucuggc cggcgucuua caggugucuc caaauuuucu caaggacaaa     2280

auuggcucag augacgaccu guucauaagu aaguggcuug uugaacacau caggggguuu     2340

aagaaagcau gcgaggauag ccugaagauu cagaaagaca accguggauu gcucaaccau     2400

aagaucaaua uugcccggaa caccaaaggu aagugugaga aggaaaucuu caaucugaua     2460

ugcaaaaucg agggcuccga agauaagaaa gggaacuaca agcacggacu agcauaugag     2520

cugggcgugc uccuguuugg ugaacccaau gaggccucua aaccugaguu cgaccgaaag     2580

auuaagaaau uuaacucgau cuacagcuuc gcucagauuc aacagaucgc guuugcagaa     2640

agaaagggga augccaacac gugugcuguc ugcuccgccg auaaugcaca uaggaugcag     2700

caaauaaaaa uuaccgagcc aguggaagac aacaaggaua aaaucauucu uucagccaag     2760

gcucagcgcu ugcccgccau cccgacacgg auaguagacg gcgcaguuaa gaaaauggcg     2820

acuauccugg cuaagaauau uguggaugac aacuggcaga auaucaaaca ggucuuaagu     2880

gccaagcacc aacuccacau uccuaucaua accgagucua acgcauucga auuugagccc     2940

gcccuggcug augugaaagg aaagagccuu aaggacagaa ggaaaaaggc ccuggagcgc     3000

auuuccccag aaaauaucuu caaagacaag aacaaccgaa ucaaagaguu cgcaaagggc     3060

auuucagcuu auagcggggc caaucuaaca gauggagacu uugauggugc gaaagaagaa     3120

cucgaccaua ucauuccucg gucucacaag aaguacggca cucugaacga ugaggccaau     3180

uugauaugcg ucacccgugg ggacaacaaa aauaagggaa acagaaucuu cugucugagg     3240

gauuuagcug acaauuauaa acucaagcag uuugaaacaa ccgaugaccu ggagauugag     3300

aaaaagaucg cagauacuau uugggacgcc aacaagaaag auuucaaguu uggcaauuac     3360

cgcuccuuca ucaaccuuac gccccaggag cagaaggcuu uucggcaugc ccuguucuug     3420

gcagacgaaa auccaauaaa acaagcggug aucagagcca uuaacaauag gaaccgaaca     3480

uuuguuaaug guacccagcg cuacuucgcu gaggugcucg ccaacaauau cuaucugcgg     3540

gcuaaaaagg agaaccuaaa cacugacaag auuaguuuug auuacuucgg gauccccacc     3600

auaggcaaug gaagaggcau ugcugaaauc aggcagcugu augagaaagu agacagcgau     3660

auccaggccu acgcaaaggg ggacaaaccu caagccucuu auucccaccu uauugaugcu     3720

augcucgcgu ucugcauagc cgcagacgaa caucguaacg auggaucaau cggucuggag     3780

auugacaaga auuacaguuu guauccacug gauaaaaaca caggcgaagu cuuuaccaag     3840

gacaucuuca gccagauuaa gaucacugau aaugaguuuu cggacaaaaa guuagugcgc     3900

aaaaaggcua uagagggguu caacacacac cggcagauga cgagagaugg aaucuacgcc     3960

gaaaauuacc ucccgauucu gauccacaaa gagcuuaacg aaguuaggaa gggcuauacc     4020

uggaaaaauu ccgaggaaau uaagaucuuu aaggguaaaa aguacgacau acagcaacug     4080

aacaauuugg uguauugucu caaauucguc gacaagccca uuucuaucga uauccagauu     4140

agcacucugg aggagcuacg aaacauccug acaaccaaua acauagccgc uacagcagaa     4200

uacuauuaca uuaaucuuaa aacucagaag cuccaugagu auuacaucga aaacuacaac     4260

accgcccugg gguauaagaa auacucaaag gagauggagu uuuugcggag uuuagcguau     4320

cgcuccgaaa gagugaaaau uaagucuauc gacgauguaa aacaagugcu ggacaaggau     4380

agcaauuuca uaauuggcaa gaucacgcuc ccuuuuaaaa aggaguggca gaggcuguac     4440

cgggaauggc agaacaccac aaucaaagac gauuaugagu uccuuaaguc cuuuuucaau     4500

guuaaaucaa uuacuaagcu gcacaagaaa guccgaaagg acuuuagcuu gccaaucagu     4560

accaacgaag gaaaauuccu agugaagaga aaaacauggg auaauaacuu cauuuaccag     4620

auacugaaug acucugauuc ccgcgcugac ggcacuaagc ccuuuauccc ugccuucgac     4680

auuagcaaaa acgagaucgu ggaggcaauc auugauucgu uuaccucaaa gaauauauuc     4740

uggcucccaa agaacaucga acugcaaaaa gucgacaaua agaacauuuu ugccaucgau     4800

acgucuaaau gguucgaggu ugaaacaccc agugaccuua gggauauugg gaucgcuacc     4860

auacaguaua agaucgacaa uaacucccgu ccgaaagugc gggugaaguu agauuacgua     4920

auugacgaug acagcaagau caauuacuuu augaaccauu cucugcucaa aagcagauau     4980

ccugauaagg ucuuggagau ucugaaacag uccacuauca uagaguucga aucaagugga     5040

uuuaacaaga caauuaaaga gaugcucggc augaagcugg cagguaucua caaugaaacc     5100

agcaacaaua agaggccagc cgcgacuaaa aaggcugggc aggccaaaaa gaaaaaguaa     5160

ggacuaguaa uaaggaaacu guaugaaugu cugugggcag gaagugaaga gauccuucug     5220

uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu uuugaaauaa uaaaucuggu     5280

agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5340

aaaaaaaaaa aaaaaaaaag aauu                                            5364


<210>  3620
<211>  3636
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3620
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagag gaacuacauu      240

cugggacucg acauagguau cacaucaguu ggcuauggga uuaucgauua cgagacacgg      300

gacgugauug augccggagu acgccuuuuc aaagaagcga auguggagaa caaugaaggc      360

cgacguucca agagaggugc uaggcggcug aagcgcagaa ggcggcaucg cauccagcga      420

gucaaaaagu uguuauuuga cuauaaccug cuaacugauc acucugagcu cucagggaua      480

aauccauacg aagccagagu gaaaggacug agucaaaaac uuagcgagga ggaguucucc      540

gcagcccugu ugcaucucgc uaaaaggcgg ggcguucaca acgugaauga agucgaagag      600

gacacgggca acgagcuguc uaccaaggaa cagaucucgc guaauucaaa ggcacuggag      660

gaaaaauaug uggccgagcu ccaguuagaa cgccugaaga aagaugggga gguaagagga      720

agcauuaaca gguuuaagac aagugacuac guuaaagagg cuaagcagcu uuugaaagug      780

caaaaggccu aucaucagcu ggaucagucc uucaucgaca cuuacauuga ucuacucgaa      840

acccggcgaa cauacuauga ggguccgggc gaagggucuc ccuuuggaug gaaggacauc      900

aaagaguggu acgaaaugcu gaugggccac ugcaccuauu ucccugagga gcuucgcagc      960

gucaaguacg cguauaaugc agaucuguac aacgccuuga augaccucaa caaucuggug     1020

auaacuagag acgaaaacga gaaauuagaa uauuacgaga aguuucagau uaucgaaaac     1080

guguucaaac aaaagaagaa accaacacug aagcagauug cuaaagagau ccucgucaau     1140

gaggaagaua ucaaggguua caggguuacc uccacgggga aacccgaguu uacuaaccuu     1200

aagguguauc acgacauuaa ggauauaacc gcccggaaag aaaucauuga gaaugcagag     1260

cuguuggacc agaucgcuaa gauacuaaca auuuaccagu caagugaaga uauccaagag     1320

gaacugacua accucaauag cgagcugacc caggaagaga ucgagcagau uucuaaccuu     1380

aaaggauaua caggcaccca uaaucugucc uugaaggcca ucaaccucau ucuggacgaa     1440

uuauggcaca cgaaugauaa ccaaauagcg aucuucaauc gccugaaacu uguaccuaag     1500

aagguggacc ucucacagca gaaagagauu ccaacuacac uggucgauga cuuuauccuu     1560

agccccgugg uuaagagaag uuucauccag ucuauuaaag ugauaaacgc caucauuaag     1620

aaauacggcu ugccuaauga uaucauuauc gaacuggcaa gggagaagaa cuccaaagac     1680

gcucaaaaga ugauaaauga aaugcagaag cguaaccggc agaccaacga gcgaaucgag     1740

gaaauuauca gaacuacagg gaaagagaau gccaaguauc ucauugaaaa gaucaagcug     1800

caugacaugc aggagggaaa augucuuuac agccuggaag cuauaccauu ggaggaucuc     1860

cugaacaauc cguuuaacua ugaggucgac cacauuaucc ccaggucggu gucuuucgau     1920

aauuccuuca acaauaaggu auuaguuaag caagaagaga acucaaagaa ggguaaucgc     1980

accccuuuuc aguaccugag uagcuccgac ucuaaaauua gcuacgaaac guucaagaaa     2040

cacauccuaa accucgccaa gggcaagggg cggauaucaa aaaccaagaa agaguaucug     2100

cuugaggaaa gagauaucaa uagguuuagu gugcagaagg acuucauuaa ccgcaauuug     2160

gucgauacac gauacgcaac ucggggacug augaaccucc ugcguuccua uuuuagagug     2220

aauaacuuag acgucaaagu gaaaagcauc aacggcgguu ucaccucuuu ucugaggcgc     2280

aaguggaaau ucaagaaaga gcggaauaag ggguacaaac aucacgccga agaugcucuu     2340

auuaucgcga acgcagacuu uauauucaag gaguggaaaa aguuggauaa ggccaaaaag     2400

guuauggaaa aucagauguu ugaggagaaa caagcugaau caaugccaga gauugaaaca     2460

gagcaggaau auaaggagau cuucaucacu ccccaucaga uuaaacacau caaggacuuu     2520

aaggauuaca aauauaguca uagaguggac aagaaaccua acagggagcu cauuaaugac     2580

acccuguaca gcacacgaaa ggaugacaaa ggcaacaccc uaauaguaaa uaaccugaau     2640

ggacucuacg auaaggacaa cgauaagcug aaaaagcuaa ucaauaaauc cccagaaaag     2700

uugcugaugu aucaccacga cccccagacu uaccaaaaau uaaagcucau uauggagcag     2760

uauggcgaug aaaagaaccc gcuguacaaa uauuacgagg aaacggggaa uuaucuuaca     2820

aaguacucua aaaaggacaa cggaccuguc aucaaaaaga uuaaguacua ugguaauaaa     2880

cugaacgccc auuuggauau caccgacgau uaccccaacu cgcgcaauaa ggugguuaaa     2940

cucagccuga agccauaucg guucgacgug uaccuagaua acggcgucua uaaauucgug     3000

acuguaaaga aucuggacgu gaucaaaaag gagaacuacu augagguuaa uuccaagugc     3060

uacgaagagg ccaaaaagcu caaaaagaua ucaaaccagg cagaauuuau ugcuaguuuc     3120

uacaauaacg accugaucaa aauuaauggg gagcuuuaua gagucaucgg agugaacaau     3180

gauuugcuga acaggauaga ggugaauaug auugacauca ccuaccguga auauuuagag     3240

aacaugaaug auaagcgccc uccccggauc auuaagacaa uagccucuaa aacccaaagc     3300

aucaagaaau acuccacuga cauucucggc aaccuguaug aagucaagag caaaaagcac     3360

ccacagauca uuaagaaagg uaagagaccc gcagcgacca aaaaggcugg gcaggccaaa     3420

aagaagaaau aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     3480

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     3540

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3636


<210>  3621
<211>  4704
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3621
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcacuguu caacaagugc      240

auuauaauca gcauuaaucu cgacuuuucc aacaaagaga aguguaugac caagccauac      300

ucuaucggac uugauauugg uacaaauuca guuggcuggg ccgugaucac ugacaacuau      360

aaaguaccga guaagaaaau gaaggugcug gggaauacga gcaaaaagua cauaaagaaa      420

aacuuguuag gaguccugcu auucgauucc ggcaucaccg cggaagguag gcggcucaag      480

cgcacagcuc gacguagaua uacuaggcgg cgcaauagga uucuguaccu ucaggagauc      540

uuuucuaccg aaauggccac acuggacgau gcauucuuuc aaagauugga cgauucguuc      600

cucgugcccg acgauaaacg ggacucaaag uauccuauuu uugggaaccu gguugaggaa      660

aaaguguacc augaugaguu cccaaccauc uaucaucugc gcaaguaccu cgccgacagc      720

acuaaaaagg cugauuuacg acuggucuac cuugcauugg cccacaugau aaaguauaga      780

ggacacuuuc ugauugaggg cgaguucaau aguaaaaaca augacaucca gaagaacuuu      840

caggacuucc uagauacaua caaugcuauu uuugaguccg accucucucu ggaaaacagc      900

aaacagcuug aggagaucgu gaaggauaaa aucuccaagc uggaaaagaa agacaggauu      960

uugaaacucu uccccggcga gaaaaauuca gggauauuca gugaauuucu gaaguuaauc     1020

guaggaaacc aagccgauuu ccggaaaugc uuuaaccugg acgagaaggc gagccuccau     1080

uucucuaagg aauccuauga ugaggaccuu gagacccugu uggguuacau uggcgaugac     1140

uauucagaug uuuuucuaaa agcaaagaaa cuguacgacg ccauccuccu gagcggguuc     1200

cuuacgguga cugacaauga aaccgaggcu ccucugaguu cugccaugau aaagcguuau     1260

aacgaacaca aagaggauuu ggcacuccug aaggaauaca uucgcaauau cuccuuaaag     1320

acauacaacg aggucuuuaa agacgauacu aagaauggau augcuggcua caucgacggu     1380

aaaaccaacc aggaggauuu cuauguguac cugaagaauc uacucgccga auuugagggg     1440

gcggacuauu uccuggaaaa gauugauaga gaggacuuuc uuaggaagca gcggacauuc     1500

gauaacggaa gcaucccaua ccagauucac uugcaagaaa ugcgagccau acuggacaaa     1560

caggcaaagu uuuaucccuu ccucgcuaag aauaaagagc gcaucgagaa gauucugacc     1620

uucagaaucc cuuacuacgu gggcccacuu gccaggggca acucggauuu ugcuuggucu     1680

auccggaaac gcaaugaaaa gauuacgccg uggaacuucg aggacgucau agauaaagaa     1740

uccucagccg aggcauuuau caauagaaug acaaguuucg accuguauuu gcccgaggaa     1800

aagguucucc cuaagcauag ccuguuauac gagacuuuua acguguauaa ugaacugacc     1860

aaaguaaggu ucauugccga guccaugcgu gacuaccagu uucuagauuc uaagcagaaa     1920

aaggacaucg ugcggcucua uuucaaagau aagcgaaagg ucacugacaa agauauuauc     1980

gaauaccugc acgcuauaua uggguacgac ggaaucgagc uuaaggguau ugagaaacaa     2040

uuuaacagcu cauugaguac auaccaugau cugcucaaca ucauuaauga caaggaguuc     2100

cuggaugacu ccagcaacga agcgaucaua gaagagauua uccacaccuu aacgauuuuu     2160

gaagauagag agaugaucaa acagaggcug ucuaaguucg agaauauauu ugacaaguca     2220

gugcuuaaaa aguugagucg ccggcacuau accggcuggg ggaaacucag cgcaaagcug     2280

aucaacggaa uuagagauga aaaauccggc aauacaaucc uagacuaccu gauugacgau     2340

gguaucucua acaggaauuu caugcagcuc auacaugacg augcccuguc guucaagaaa     2400

aagauucaaa aggcucagau caucggggac gaggauaaag gcaacauuaa ggaaguugug     2460

aaaagccuuc caggaucccc cgccaucaag aaaggcauuu ugcagucaau aaagaucguc     2520

gacgagcugg ugaagguaau gggagggcgc aaaccugaaa guauuguugu ggagauggca     2580

cgagagaauc aguauacuaa ccaagguaag ucuaauagcc agcagcgguu aaaacgucuc     2640

gaaaaguccc ugaaagagcu uggcucaaag auccugaagg aaaacauucc agccaaauug     2700

agcaagaucg auaauaacgc ucuccagaau gacagacugu accuauauua ccugcaaaac     2760

gggaaagaua uguauaccgg agacgaucuc gacauagaca ggcugucuaa cuacgauauc     2820

gaccacauua ucccccaggc cuuucuuaag gauaauucca uugacaacaa agucuuggug     2880

aguagcgcau cuaaucgcgg caaguccgau gacuucccgu cacuggaggu cgugaagaaa     2940

cggaagacau uuugguacca guuacucaaa aguaagcuga ucagccagag aaaauucgau     3000

aaccuuacua aggcggagag gggugggcug cuaccugaag acaaggcugg cuuuauacaa     3060

cgacagcucg uugagacccg ccagauuaca aaacaugugg cccggcuguu ggaugaaaag     3120

uucaauaaca aaaaggacga gaauaacaga gcaguaagga ccgucaaaau caucacucug     3180

aagucgacgu uaguguccca guuucguaaa gauuucgaac ucuauaaggu ucgcgagauu     3240

aaugacuuuc accaugccca cgaugcuuac cugaacgccg ugauagcauc ugcccuucug     3300

aagaaauauc ccaaguugga gccagaguuc gucuacggag acuauccuaa auacaauagc     3360

uuucgggaaa gaaagucagc uacagaaaaa guguauuucu acaguaacau caugaauauu     3420

uucaagaagu ccaucucucu cgcggacggc aggguaauug agcgaccccu gaucgaagug     3480

aacgaggaga ccggggaaag cguuuggaau aaagaguccg aucuagccac uguccggcgc     3540

gugcugucau acccacaagu gaacgucguu aagaaagugg aagaacagaa ccacggacuu     3600

gacagaggua agcccaaagg ccucuuuaau gcuaaccuga gcaguaagcc uaagccaaau     3660

ucuaacgaaa auuugguggg ggcaaaagag uaucuggauc cgaagaaaua cggaggcuau     3720

gccgguauau ccaacagcuu cgcuguauua gucaagggga ccaucgagaa aggcgccaag     3780

aagaaaauua caaaugugcu cgaauuucag ggaaucucga uucuggacag gaucaacuac     3840

cggaaggaua aacuuaauuu ccuguuggag aagggcuaua aagacauaga acucauuauc     3900

gagcugccca aguacucacu auuugagcug ucugauggga gucgaagaau gcuugcaucc     3960

auccucagca ccaacaauaa acgcggagaa auucauaagg gcaaccaaau cuuccugucu     4020

cagaaguuug uuaaauuguu auaucacgcg aagaggauaa gcaauacuau uaacgagaau     4080

caucguaaau acguggaaaa ccacaagaaa gaguucgagg aacuguuuua cuauauccuc     4140

gaguucaaug aaaacuacgu cggugccaag aagaacggga aacugcuuaa uuccgcuuuu     4200

cagucauggc agaaccacag uauugacgag cuguguagcu cuuucaucgg accuacaggc     4260

uccgaacgga aggguuuguu ugagcuaacg ucaagaggca gugccgcaga uuucgaguuc     4320

cuggggguga aaauaccaag guaucgcgac uacaccccca gcucccuccu gaaggaugcu     4380

acucuuauuc aucaaucugu uacaggauua uaugaaaccc gaaucgaccu ggccaaacuc     4440

ggcgagggga agagaccugc agccacaaag aaagcuggac aggcgaagaa aaagaaauaa     4500

ggacuaguaa uaaggaaacu guaugaaugu cugugggcag gaagugaaga gauccuucug     4560

uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu uuugaaauaa uaaaucuggu     4620

agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaag aauu                                            4704


<210>  3622
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3622
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcauggua cgccagccug      240

auguccgcgc aucaccucag gguuggaauu gacgugggua cccauucugu aggccuugcu      300

acacugcggg uggaugacca cgggacucca auagaguugu uaucagcccu gagucauauc      360

cacgauagcg gagucggcaa ggaagguaaa aaggaccacg auacgcgcaa gaaacuaucc      420

gggauugcac gacgugccag aaggcuccug caucaccggc gcacccagcu ucaacagcug      480

gacgaggugu ugagagaucu cggauucccg auccccacac cuggcgaauu ucuggaccug      540

aacgagcaga cugauccaua uaggguuugg cgggugcgcg cucgacucgu cgaagagaag      600

uuacccgagg aacugagagg cccugcaauu ucuauggccg ugaggcauau cgcucggcac      660

cguggguggc gcaauccaua cucgaaagua gagucacuuu ugagccccgc cgaagagagu      720

ccuuucauga aggcgcugag agagaggaua cuagcaacca caggagaagu ucucgacgau      780

gguaucaccc caggccaggc cauggcucaa guggcccuga cucacaacau uuccaugcgg      840

gggccggagg gaauccuugg caaacugcau cagucugaca augcaaacga aauucgaaag      900

aucugcgcuc gccagggugu cagccccgau guguguaagc aguugcucag agccguguuu      960

aaagcggacu ccccuagggg gucagccguc agucggguug caccagaccc ccugccugga     1020

caaggcagcu uccgcagagc uccaaagugc gaucccgagu uucagagguu ccguauaauu     1080

ucuaucgugg ccaauuuacg gauuuccgaa acaaaaggcg agaaccgacc gcugaccgcu     1140

gacgagagaa ggcacguagu gacguuucuc acugaagauu cacaggccga ccuuaccugg     1200

gucgaugugg cagagaagcu ggggguucau cgccgggacu ugagaggaac agccgugcac     1260

acugaugacg gugaaaggag cgcugcgcgc ccucccaucg augcaaccga ccgaaucaug     1320

cggcagacaa aaauuaguuc ucuaaagacc uggugggagg aagccgauuc cgagcaacgu     1380

ggcgcuauga uaagauaucu guacgaggac ccaacggaca gcgaaugugc cgagaucauu     1440

gcagaacucc cugaggaaga ucaggccaaa cuggacucgc uucaucugcc cgcugggagg     1500

gccgcauauu cucgcgaguc cuugacugcg cucucagauc acaugcuggc uacaaccgac     1560

gauuuacacg aggcccggaa gagacuguuc ggagucgacg auaguugggc accacccgcc     1620

gaagcuauca augccccugu gggcaaccca agcguagaca ggacucuaaa gauaguuggu     1680

cgauaccucu ccgcagugga gucuaugugg gggacaccgg aagucauuca uguggagcac     1740

guccgcgaug gcuuuaccag cgagcggaug gcugacgaaa gagauaaagc caauaggcgu     1800

cgcuauaacg acaaucagga ggcgaugaag aaaauccaac gggauuacgg aaaggaaggc     1860

uacaucucaa gaggggacau ugugaggcug gacgcccuug aguugcaggg augcgcuugu     1920

cuguauugcg guacgaccau cggcuaccau acaugucagc ucgaucacau uguuccccag     1980

gcagggccug gaaguaacaa ucgacggggc aaccuggugg ccguaugcga acgcuguaau     2040

agauccaaaa gcaacacucc auucgcuguc ugggcccaaa agugcgguau accccacgug     2100

gggguuaagg aggcaaucgg cagggugcgg ggauggcgaa aacagacccc uaacacaucu     2160

ucagaggacc uuacuagacu gaagaaagaa gucauugcgc gcuugaggcg uacccaggag     2220

gauccagaaa ucgacgagcg gaguauggaa agcguggccu ggauggcuaa ugagcuccau     2280

cacagaaucg ccgcagcuua ucccgagaca accguaaugg uguacagggg cuccauuacu     2340

gccgcagccc gcaaggcugc ggggauagau ucucgaauca accugauugg agaaaaaggu     2400

agaaaggacc ggaucgauag gcgccaucac gccguugacg caucggucgu ggcuuuaaug     2460

gaggccagcg uggccaagac gcuggcugaa cguuccucac uaagaggcga gcagcggcuc     2520

acagggaaag aacaaaccug gaagcaguau acuggaagua ccgucggcgc aagggagcau     2580

uuugagaugu ggcgcgguca caugcugcac cuuacagaau uguucaauga gcgacuggcc     2640

gaagauaaag uuuacgugac ccagaacauu agacuccggc ugucugacgg gaaugcgcau     2700

acugugaacc cgagcaaguu aguaucccac aggcugggcg auggacuuac aguccagcaa     2760

aucgaccgcg cuuguacgcc ugccuugugg ugcgcacuca ccagagagaa agauuuugac     2820

gagaagaaug gccugccagc cagggaagac cgggcuauac gugugcaugg gcacgagauc     2880

aagucaagcg auuauauuca gguuuucucu aaacgcaaga aaacugacuc cgaucgagac     2940

gaaacacccu ucggagcaau cgccgugaga ggcgguuuug ucgagauugg gccuaguauc     3000

caucacgcga ggauauaccg gguggaagga aagaaacccg uuuacgcuau gcuacgcgug     3060

uucacccacg aucugcucag ccagagacau ggcgaccugu uuucugccgu aauuccaccu     3120

caguccaucu caaugaggug cgcagagccc aaacuucgga aagccauuac aacugguaac     3180

gcuaccuauu ugggcugggu cguggugggg gaugagcugg aaaucaaugu cgacaguuuc     3240

acgaaguacg ccauaggacg auuuuuagag gauuucccaa acaccacaag auggcgcauc     3300

uguggcuaug acacuaauag caagcucacc cugaaaccga uuguucuugc agcugaaggg     3360

cuggagaacc cuucguccgc cgugaaugaa aucguagagu ugaagggaug gaggguggcg     3420

auuaacgucc ucacaaaagu gcaccccacu guugugcggc gugaugcccu gggcagacca     3480

agguacucua gccgcucaaa ucuacccacc aguuggacga ucgagaagcg gccugcagcu     3540

acaaagaagg ccggucaagc uaagaaaaag aaauaaggac uaguaauaag gaaacuguau     3600

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     3660

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3780


<210>  3623
<211>  4641
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3623
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaccaa gccauacagc      240

auuggacugg acauagguac aaacuccguu ggcugggccg ugacuacgga uaauuauaaa      300

guaccgucua agaagaugaa agugcucggg aacaccucaa agaaauacau caagaaaaau      360

cuucugggag ucuuguuauu cgacaguggc auuacagcgg aggguaggcg gcugaagcgc      420

acugcucgac guagauauac caggcggcgc aacagaaucc uauaccucca ggaaauuuuu      480

agcacagaga uggccacccu ggaugacgca uucuuucaaa ggcuugauga cuccuuccug      540

gugcccgaug acaagcggga uucuaaauau ccuaucuuug ggaauuuggu ugaagagaag      600

gccuaccaug acgaguuccc aacuauauau caccuccgca aauaccuggc ugauucgaca      660

aagaaagcag accugcgacu cguguacuua gcccuggcuc auaugaucaa guauagagga      720

cacuuucuua uugagggcga guucaacuca aagaauaacg auauccagaa gaauuuucag      780

gacuucuugg acaccuacaa cgccauuuuu gaaagcgauc ugagucuaga aaauuccaag      840

cagcucgaag agaucgucaa agacaagaua ucuaaacugg agaagaaaga uaggauucuu      900

aagcuguucc ccggcgaaaa gaacagcggg aucuuuuccg aguucuugaa acucauugug      960

ggaaaucaag cggacuuccg gaagugcuuu aaccuggaug aaaaagcauc auuacauuuc     1020

aguaaggaga gcuaugacga agaucuggag acgcuccuug guuacaucgg cgacgauuau     1080

ucugacguau uucugaaagc caagaaguug uacgaugcua uccuacuguc cggguuccuc     1140

acuguuaccg acaaugagac agaagccccu cugucaagcg caaugauuaa acguuauaac     1200

gagcacaagg aagaccuugc ucuguugaaa gaguacauac gcaacaucag ucucaagacu     1260

uacaaugaag uguuuaaaga ugacaccaag aacggauaug ccggcuacau ugaugguaag     1320

acaaaucagg aggacuucua ugucuaccug aaaaaguuac uggcggaguu ugaaggggcc     1380

gauuauuucc uagagaaaau cgacagagaa gauuuucuca ggaagcagcg gaccuucgac     1440

aacggaucua uaccauacca gauucaccuu caagagaugc gagcaauccu ugauaaacag     1500

gcuaaguuuu aucccuucuu ggccaaaaau aaggaacgca ucgagaagau ucugacguuu     1560

agaaucccuu acuacguggg cccacucgcu aggggcaacu ccgacuucgc cuggagcauu     1620

cggaaacgca augagaagau aacuccgugg aacuucgaag augugaucga caaagagucg     1680

ucugcagaag ccuuuauuaa uagaaugaca uccuucgauc uguaucuucc cgaggagaag     1740

guccugccua aacauucauu gcucuacgaa accuuuaacg uuuauaauga gcugacuaag     1800

gugagguuca ucgcugaaag uaugcgugac uaccaguuuu uagacagcaa gcagaaaaag     1860

gauaucguac ggcuguauuu caaagacaag cgaaaaguga cagauaagga cauuauagag     1920

uaccuacacg cgaucuaugg guacgaugga auugaacuca aggguaucga gaaacaauuu     1980

aacuccucuc ugagcaccua ccaugaccuu uugaauauua ucaacgauaa ggaguuccug     2040

gacgauucaa guaaugaagc aauaaucgag gaaauuaucc acacgcucac cauuuuugag     2100

gacagagaaa ugaucaaaca gaggcugucc aaguucgaga acauauuuga uaaaagcguc     2160

uuaaagaagc ugucucgccg gcacuauaca ggcuggggga aacuuucagc caaguugauu     2220

aacggaauca gagacgagaa aaguggcaau acuauucucg auuaccugau cgacgacggu     2280

auaagcaaca ggaauuucau gcagcuaauc caugaugacg cucuguccuu uaagaaaaag     2340

auucaaaaag cccagaucau uggggaugaa gacaagggca acaucaagga ggugguuaaa     2400

ucucucccag gaucgcccgc aauaaagaaa ggcauucugc agagcaucaa gaucguggau     2460

gaacuuguca aagugauggg agggcgcaag ccugagucca uuguaguuga aauggcccga     2520

gagaaucagu auaccaacca agguaaguca aauagucagc agcgguugaa acgucuggag     2580

aagucuuuaa aagaacucgg cagcaagauc cugaaagaga acauuccagc uaagcugucc     2640

aagauagaca auaacgcccu gcagaaugau agauuguacc ucuauuaccu gcaaaacggg     2700

aaagacaugu auacaggaga ugaccuagau aucgacaggc ugucaaauua cgacauugau     2760

cacaucauuc cccaggcauu ccucaaggac aacagcaucg auaauaaagu gcuggucucu     2820

uccgcgagua accgcggcaa gagcgacgau gugccgucuc uugaagucgu gaaaaagcgg     2880

aagacuuucu gguaccaguu gcugaaaucc aaguuaauau cacagagaaa auuugacaac     2940

cucaccaagg cugagagggg ugggcugagu ccugaggaua aagccggcuu cauccaacga     3000

cagcuuguug aaacacgcca gauuaccaag cauguggcac ggcugcuaga cgagaaguuu     3060

aauaacaaaa aggaugaaaa uaacagagcc guaaggacug ucaaaaucau uacgcucaag     3120

agcacacugg ugucgcaguu ccguaaagac uuugaguugu auaagguucg cgaaaucaau     3180

gauuuccacc augcucacga cgccuaccug aacgcagugg ucgcuuccgc cuuacucaaa     3240

aaguauccca agcuggagcc agaguuugug uacggagauu auccuaaaua caauucuuuc     3300

cgggaaagaa agagcgcgac cgagaaagua uauuuuuacu caaacauaau gaauauuuuc     3360

aagaaaagua ucucccuugc cgacggcagg gugaucgaac gaccccugau ugagguuaac     3420

gaagagacug gggagucugu cuggaauaag gaaagcgacu uggcuaccgu gcggcgcgug     3480

cucuccuacc cacaagucaa cguugugaag aaaguggagg aacagaauca cggacuggau     3540

agagguaagc ccaaaggccu auuuaacgca aaucugucaa gcaagccuaa accaaacagu     3600

aacgagaauc uuguaggggc caaggaauau cucgacccga agaaauacgg aggcuaugcu     3660

gguauaucua acuccuucac aguccuggug aaggggacca ucgagaaagg cgccaagaaa     3720

aagauuacua auguuuugga guuccaggga aucagcauuc uggauaggau caacuaccgg     3780

aaggacaaau uaaauuuucu ccuggaaaag ggcuauaaag auauagagcu uaucauugaa     3840

cugcccaagu acucguuguu cgagcucuca gacgggucuc gaagaaugcu ggcaaguauc     3900

cuauccacaa acaauaaacg cggagagauu cauaagggca accaaaucuu ucugagccag     3960

aaauucguga aacuucucua ucacgcgaag aggauaucua auacgauuaa cgaaaaucau     4020

cguaaauacg ucgagaacca caagaaagaa uuugaggaac uguucuacua uaucuuggag     4080

uuuaaugaga acuacguggg ugccaagaaa aaugggaagu uacugaacag cgcuuuccag     4140

uccuggcaga aucacucaau cgaugaacuc uguaguagcu uuauuggacc uaccggcucu     4200

gagcggaagg gucuguucga acuuacuucc agaggcucag ccgcagacuu ugaguuccug     4260

gggguuaaaa ucccaaggua ucgcgauuac acacccagua gcuugcuaaa ggacgcuacc     4320

cugauacauc aauccgugac aggacucuau gaaacucgaa uugaucuggc caaacuuggc     4380

gaggggaaga gaccugcagc caccaaaaag gcuggacagg cgaagaaaaa gaaauaagga     4440

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     4500

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     4560

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaagaau u                                               4641


<210>  3624
<211>  3723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3624
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagccgc guucaagcca      240

aacagcauua auuacauacu gggacucgac aucgguauug cuuccguugg cugggccaug      300

guggagaucg augaagagga aaacccgauu aggcuuaucg accugggggu acggguguuu      360

gagcgcgcag aaguccccaa aaccggagau ucuuuggcca uggcucgacg uuuagcaaga      420

ucagugaggc ggcugacacg cagaagggcc caucggcuac uccgcacucg aagacugcuu      480

aagagggagg gcguucugca ggcugccaau uucgacgaga acgguuugau aaagagucuc      540

ccuaauacgc cauggcaacu gcgggcggca gcccuggauc guaaacucac ccccuuagaa      600

uggagcgcug ugcugcuuca cuugaucaag caucgcgggu aucuguccca gaggaaaaac      660

gagggagaaa cagccgacaa ggagcuaggc gcacuccuga aaggcgucgc ugggaaugcc      720

cacgcgcuuc agacuggaga uuuuaggacc ccugccgagc uggcauugaa caaguucgaa      780

aaggagucug gucauauucg gaaucagcga ucggacuacu cacacacauu uagccgcaaa      840

gaucuccaag cugaacugau cuuacuguuc gagaagcaga aagaauuugg caacccacac      900

gugagugggg gacucaagga gggcauugag acccuucuga ugacucagag acccgccuug      960

uccggugacg cuguacagaa aaugcuaggg cauugcacau ucgaaccugc cgagccaaag     1020

gcagccaaaa auaccuauac ggcugaaagg uuuaucuggc ugacuaagcu caacaaucug     1080

cggauacuug agcaaggauc ugaacgcccg cugaccgaua cagagagagc gacuuugaug     1140

gacgagcccu acagaaagag caaacucacc uaugcacagg cccguaagcu guuaggccug     1200

gaagauacag cuuucuuuaa aggccuacgg uacgggaagg acaacgccga ggcauccacc     1260

cucauggaaa ugaaagccua ucacgcuauu ucacgagccc uggagaagga aggacuuaag     1320

gacaaaaaga guccuuugaa ucugagccca gagcuccagg augagaucgg uacggcauuc     1380

ucucuguuua aaacugacga agauauuaca ggcagacuua aggacaggau ccagcccgag     1440

auccuggaag cguugcucaa acauauuucc uucgauaagu ucguucaaau aucacugaag     1500

gcuuuacgcc ggaucgugcc ucugauggag caggggaaaa gauacgacga ggccugugca     1560

gaaauuuacg gagaucacua uggcaagaaa aacaccgagg aaaagaucua ccuaccaccc     1620

auaccggccg acgagauuag gaacccuguc gugcuccgcg cucugagcca ggcccgaaaa     1680

gugaucaaug gugucguucg gcguuauggg agucccgcaa gaauccacau ugaaacugcu     1740

agggaggugg gcaagucuuu uaaggaucgc aaagagaucg aaaagcggca agaggaaaac     1800

agaaaagaca gggagaaggc cgcggccaaa uuccgagaau acuuuccaaa uuucguagga     1860

gagccuaagu ccaaagauau ucuuaaguug cgccuguaug agcagcagca uggcaagugc     1920

cucuacagcg ggaaagaaau aaaccuggga cgguuaaaug agaaggguua uguggaaauc     1980

gaccacgcuc ugcccuuuuc gagaacaugg gacgauucuu ucaacaauaa aguccuugug     2040

uugggcuccg agaaccagaa uaaggggaac caaaccccau acgaauacuu uaauggaaaa     2100

gacaacucaa gggaguggca ggaguucaag gcacguguug aaacgagucg cuuuccccgg     2160

agcaagaaac agaggauucu ccugcagaag uucgaugagg acggcuuuaa agaaagaaau     2220

cuaaacgaua cccgauaugu gaaucgguuc cugugucaau uugucgccga ccgcaugaga     2280

cucacaggua aggggaaaaa gaggguguuc gcuuccaacg gccagaucac uaaccugcuu     2340

cggggauucu ggggcuugcg aaagguaaga gccgagaaug aucgccauca cgcacuggac     2400

gcgguugugg ucgccugcuc uaccguggcu augcagcaga aaaucacaag guuuguccgu     2460

uacaaggaga ugaacgccuu cgaugggaaa acuauugaca aggaaaccgg agagguguua     2520

caucagaaaa cacacuuucc ucagccaugg gaguucuuug cacaggaagu uaugauacgg     2580

guguucggua agccggaugg caagcccgaa uuugaggagg cugacacccu cgaaaaacug     2640

agaacucuuc uggccgagaa guugagcuca aggccugaag caguacacga guaugucacg     2700

ccacucuucg ugagucgcgc ccccaaucga aaaauguccg ggcagggaca uauggaaaca     2760

guuaagagcg cuaaaagacu ggaugagggc gugucugucc uacgggugcc ucugacccaa     2820

cucaagcuga aagaccuuga gaagauggua aacagggaac gcgagccaaa guuguacgaa     2880

gcgcugaaag cccguuuaga ggcacacaag gacgaucccg cuaaagccuu ugccgaaccg     2940

uucuauaagu acgacaaagc ugguaauaga acucaacagg ugaaggcagu ucgggucgag     3000

caagugcaga agaccggggu gugggucagg aaccauaaug gcaucgccga uaacgcgaca     3060

augguucgcg uggacguguu ugagaaagga gauaaguauu accucguacc uauuuacuca     3120

uggcaggucg cuaaaggcau ccugccagac cgagccgugg uucaggggaa ggaugaagag     3180

gacuggcaac uuauugauga caguuucaau uuuaaauuca gccugcaccc caacgaucua     3240

guggaaguca ucaccaagaa ggcaagaaug uucggauauu uugccuccug ucaccggggc     3300

acugguaaua uaaacaucag gauucaugac cucgaucaca aaaucgggaa gaauggcauu     3360

cuggagggaa ucggugugaa aacagcuuug ucuuuccaga aguaccagau agacgagcug     3420

ggcaaagaaa uucgcccuug cagauuaaag aagaggcccc caguucggaa acguccugca     3480

gccacgaaga aagcggggca ggcuaagaaa aagaaguaag gacuaguaau aaggaaacug     3540

uaugaauguc ugugggcagg aagugaagag auccuucugu aaagguuuuu ggaauuaugu     3600

cugcugaaua auaaacuuuu uugaaauaau aaaucuggua gaaaaaugag aucuaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     3720

auu                                                                   3723


<210>  3625
<211>  4479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3625
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagaa accauacacc      240

auuggacugg acauagguac aaacagcguu ggcugggccg ugcucacuga ucaguaugac      300

cuuguaaaga ggaagaugaa aaucgcgggg gauuccgaga agaaacaaau uaagaaaaau      360

uucuggggag ugcggcuguu ugacgaaggc cagacggcug ccgaucgccg aauggcacgu      420

accgccagaa ggcggaucga gcgcagaagg aaccggauuu cuuacuugca ggguaucuuc      480

gcugaagaga ugucaaagac agacgcaaac uuuuucugcc gcuuaaguga uagcuuuuau      540

gucgacaaug aaaagcgaaa uuccagacau ccguucuuug ccacuauaga ggaggaagug      600

gaguaccaca aaaacuaucc caccaucuac caucugaggg aggagcuagu uaauucuucg      660

gaaaaggcug aucuccggcu gguguaucuu gcccuggcgc acauuaucaa auaccguggg      720

aacuucuuga uugaaggagc acucgacaca cagaauaccu cagucgaugg caucuacaag      780

caauuuauac agacuuauaa ccagguguuc gccagcggca uugaggacgg gagucugaaa      840

aagcuggaag auaauaaaga cguagcuaag auccucguug agaaggugac acgcaaagaa      900

aaguuagaga ggauucugaa acuuuacccu ggagagaagu ccgccgguau guuugcacag      960

uucaucucuu ugaucgucgg cagcaaaggg aacuuucaaa agccauucga ccugauugaa     1020

aaguccgaua uagagugugc uaaagacuca uaugaagagg aucuagaaag ucuccuggcc     1080

cuuaucggag acgaguacgc ggagcuguuc guggccgcaa agaaugcuua uagcgccgug     1140

gucuugucuu ccauuaucac cguugcugaa acggagacua acgccaaacu cucagcaagc     1200

augauagaaa gguuugauac ccaugaggaa gaccugggcg aguuaaaggc cuucauuaaa     1260

cugcaccucc ccaagcacua cgaggaaauc uuuaguaaua cagagaagca ugguuaugcu     1320

ggguacaucg auggaaaaac uaagcaggcg gacuucuaca aauauaugaa gaugacccuu     1380

gaaaacauug agggcgcaga uuacuuuauc gccaaaauug agaaggaaaa cuuccugcgg     1440

aagcagcgaa cauuugacaa uggcgcuaua ccucaccagu ugcaucuaga ggaacuggag     1500

gccauccucc accaacaggc aaaauauuac ccauuccuga aggaaaacua ugauaaaauu     1560

aagucucuug ugaccuuucg cauccccuac uucguagggc cucuggccaa uggacagucc     1620

gaguuugcuu gguugacgag aaaagccgac ggugagauca ggccauggaa cauugaagag     1680

aagguggacu ucggcaaaag cgcagucgau uuuauagaaa agaugacuaa uaaggacaca     1740

uaucucccga aagagaacgu gcugcccaag cacucguuau gcuaccaaaa auaccugguu     1800

uauaaugaac uaaccaaggu gcgguacauc aacgaucagg ggaaaacuuc uuauuucucc     1860

ggacaggaga aggagcagau uuucaaugac cucuuuaagc aaaaacgcaa ggucaaaaag     1920

aaagaucugg aacuuuucuu gagaaacaug ucacaugugg agaguccuac aaucgaaggc     1980

cuggaggaca gcuuuaauuc cucuuacagc accuaucacg aucuccugaa gguagguauu     2040

aagcaggaaa uccuugacaa cccaguuaau acggagaugc uggagaacau agugaaaauc     2100

uugaccgucu ucgaagauaa gaggaugauu aaagagcagc uccagcaauu uucagacgug     2160

cuggaugggg ucguguuaaa gaaacuggaa cgucggcauu acacaggcug gggacgacua     2220

agugcgaagc uccugauggg caucagagac aagcaguccc accuuacuau uuuggauuau     2280

cugaugaaug acgacgggcu caacaggaac cugaugcagu uaaucaauga uagcaaccug     2340

ucuuucaaau caauaauuga gaaggagcag guuaccacag cugacaaaga uauccaaagu     2400

auuguggccg accuugcagg aagccccgcc aucaagaaag guauauugca gucccucaag     2460

aucguagaug aacuggucuc ugugaugggc uacccuccac agacuauugu uguggagaug     2520

gcucgcgaaa aucagaccac agggaaagga aagaacaauu cgcggcccag auacaagagc     2580

cuagagaaag ccaucaagga auuuggcucc caaauucuga aagagcaucc gaccgacaac     2640

caggagcuca ggaauaaccg ccuguaucuu uacuauuugc agaaugguaa ggauauguac     2700

acugggcaag accuggauau ccacaacuua ucaaauuaug acauagauca cauugucccu     2760

cagaguuuca ucacggacaa cucuaucgau aaucucgugc ugacaagcuc cgcaggcaac     2820

cgagaaaaag gagacgacgu acccccacuu gagauugugc ggaagcguaa ggucuuuugg     2880

gaaaaacugu accagggcaa uuugauguca aagagaaaau ucgauuaucu caccaaggcu     2940

gagagagggg gacugacuga agccgacaaa gcgcgcuuua uccaucggca gcuaguugag     3000

accagacaaa uuacaaagaa cguggccaac auacugcacc agagguucaa uuacgagaag     3060

gaugaccaug guaacaccau gaaacaggug cgaaucguca cucucaagag cgcucugguu     3120

ucucaguuuc gcaaacaauu ccagcuuuac aaggugcggg augugaauga cuaucaccac     3180

gcacaugaug ccuacuugaa cggcguaguc gcuaauacac uguuaaaagu guauccucag     3240

cucgaacccg aguucguuua cggggacuau caccaguuug auugguucaa ggccaacaag     3300

gcaacggcga aaaagcaauu uuacaccaau auuaugcugu ucuuugccca gaaagacaga     3360

aucauugaug aaaacggaga gauccuuugg gacaagaaau aucuggacac ugugaagaag     3420

gucauguccu acaggcagau gaauauagug aaaaagacag aaauccaaaa aggcgaguuc     3480

aguaaggcua ccauuaaacc aaaggguaac agcucuaaac uaaucccccg uaagacaaau     3540

ugggauccua ugaaguacgg gggccucgac uccccaaaca uggccuaugc aguugugauu     3600

gaguacgcua aaggaaagaa uaaacuggua uuugaaaaga aaaucauacg cgucacuauu     3660

auggagcgga aggccuucga aaaggaugag aaagcauuuu uggaggaaca gggcuauaga     3720

cagccgaagg ugcuggccaa auuacccaag uacacccucu augaguguga agaggggagg     3780

cgacggaugc uggcuucagc gaacgaagcc cagaaaggaa aucaacaggu gcuuccuaac     3840

caucugguca cguugcucca ccaugcagcu aacugcgagg uuagugacgg caagagccug     3900

gauuacaucg agucgaaucg cgaaauguuc gccgagcuac uggcccacgu guccgaauuu     3960

gcuaagagau auacccuugc agaggccaac cucaauaaaa ucaaccagcu guucgaacag     4020

aauaaggagg gugacauuaa agcgauagcu caaucuuucg uagauuugau ggccuuuaac     4080

gcaauggggg ccccagcuag cuucaaguuu uucgagacaa cuaucgaaag gaaacgguac     4140

aauaaccuga aggaguuacu caauucaacc auuaucuacc agaguauuac aggacuguau     4200

gaaucccgaa agagacuuga cgauaaacgc cccgcagcca cuaagaaagc gggccaggcu     4260

aagaaaaaga aauaaggacu aguaauaagg aaacuguaug aaugucugug ggcaggaagu     4320

gaagagaucc uucuguaaag guuuuuggaa uuaugucugc ugaauaauaa acuuuuuuga     4380

aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            4479


<210>  3626
<211>  4512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3626
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagaa accauacagc      240

auuggacugg acauagguac caacuccguu ggcugggccg ugguaacaga ugacuauaag      300

gugccggcga agaaaaugaa gguccucggg aauacugaua aaucucauau cgagaagaac      360

cuucugggag cuuuguuauu cgacucaggc aauacggccg aagauaggcg gcugaaacgc      420

accgcacgac guagauacac aaggcggcgc aaccggauuc uauaucucca ggagaucuuu      480

agugaagaga uggguaaggu ggacgauagc uucuuucaca ggcuggaaga cuccuuccuu      540

guuacugagg auaagagagg ggagcgccau cccauuuuug gaaaucugga agaggaagug      600

aaauaccacg agaacuuccc uaccaucuau cauuugcgac aauaccucgc cgacaaucca      660

gagaaggucg aucugagacu gguguaucuc gcuuuagcac acauaaucaa auuuaggggc      720

cacuuccuga uugaaggcaa guuugacaca cggaacaaug auguacagcg ucuuuuccag      780

gaguuuuugg ccguuuacga caacaccuuc gaaaauucuu cgcugcagga gcaaaacgug      840

caggucgaag agauccuaac ugacaaaauu ucaaagagcg cuaaaaagga ucgcgugcuc      900

aagcuguuuc ccaaugagaa aaguaacggg agauucgccg aguuccuuaa gcugaucgug      960

ggaaaucagg cggacuuuaa aaagcauuuc gaguuggaag agaaagcacc ucuccaguuu     1020

uccaaggaua cauacgaaga ggagcuggaa gucuuacugg cccaaauagg ugacaacuau     1080

gcugaacucu uccuuucugc caagaaacug uacgauagca uuuugcuauc cggcauccug     1140

accguuacgg acguggggac uaaggcacca cucucagcua guaugauuca gagguauaac     1200

gaacaccaga uggaucuggc ccagcuuaaa caauuuaucc ggcagaagcu gagcgacaaa     1260

uacaaugagg uauucucuga uguguccaag gacggauaug cgggcuacau cgaugguaag     1320

accaaccagg aagccuuuua uaaauacuug aaggggcucc ugaauaaaau ugagggauca     1380

ggcuacuucu uagacaagau agagcgagaa gacuuucugc gcaaacaaag aacauucgau     1440

aacggcagca ucccccauca gauucaccua caggagauga gggcaaucau acggcgccag     1500

gcugaauuuu auccuuuccu cgccgacaau caagauagaa uugagaagcu gcuuacuuuu     1560

aggaucccau acuaugucgg gccguuggcu cguggaaaga gugacuucgc cuggcugucu     1620

cggaaauccg cagauaagau cacccccugg aacuucgacg agauugugga uaaagaaagc     1680

ucggccgagg cuuuuaucaa ucgaaugaca aacuacgacc ucuaucugcc uaaucagaag     1740

guucuuccaa aacacucucu guuguacgaa aaguucaccg uguauaacga gcucacgaaa     1800

gucaaguaca agacugaaca ggguaaaaca gcguuuuucg augcaaauau gaagcaggag     1860

auuuuugacg gcguguucaa aguauacaga aagguuacca aagauaagcu gauggacuuu     1920

uuagagaagg aguucgauga auuuaggaua guggaccuga cugggcuaga caaagaaaac     1980

aaggucuuca augccuccua uggaacauac caugaucucu gcaaaauccu ggacaaggau     2040

uuucuugaca acaguaaaaa ugagaagauu uuggaagaua ucgugcugac ccucacgcug     2100

uucgaggacc gcgagaugau ccggaagaga uuagaaaacu auucagaucu gcuuaccaaa     2160

gagcaaguca agaaauugga aaggcgccac uacacaggcu ggggucgacu cagcgcugag     2220

cugauucaug ggauacggaa caaggaaucc cguaaaacua uccuagacua ucugauugau     2280

gacggcaauu cuaacagaaa uuuuaugcag cucaucaacg augacgcccu gagcuucaag     2340

gaggagauug caaaggccca ggugaucgga gaaaccgaua aucuuaacca gguuguguca     2400

gacauagcug gcagucccgc caucaaaaag gggauuuugc aaucccugaa aaucguagac     2460

gaguuaguca agauuauggg acaccagccu gaaaauaucg ugguugagau ggcaagggag     2520

aaccaguuca caaaucaggg ucgccggaac agccaacaga gacucaaagg ccugacugau     2580

ucuauaaagg aauuuggguc acagauucuu aaagagcauc caguggaaaa uagucaacug     2640

cagaacgaca gguuguuccu cuacuaucug cagaauggac gagauaugua caccggcgag     2700

gaacuagaca ucgauuaccu gagccaguau gacauugauc acaucauacc ccaagcguuu     2760

aucaaggaca acuccauuga uaaucgcguc cucacaucuu cgaaggagaa ccgggguaaa     2820

agcgacgaug ugccguccaa ggacguagug agaaaaauga agucauacug gaguaaacug     2880

cuuucugcua aguugaucac ccagaggaag uucgacaacc ugacuaaagc cgagcguggg     2940

ggcuuaacgg augacgauaa ggcaggauuu auuaaacgcc agcucguuga aacacggcag     3000

aucaccaagc acgucgccag aauacuggac gagagguuca auacugaaac cgaugagaac     3060

aauaaaaaga uucgacaagu gaagaucgug acacuuaaaa gcaaccuggu cuccaauuuu     3120

cggaaggagu ucgaacuaua uaaaguucgc gagaucaacg acuaccauca cgcucaugau     3180

gccuaucuca augcagugau uggcaaggcu cuguuggggg uguacccuca gcuggaaccc     3240

gaguuuguau auggagacua cccacacuuc cacggucaua aagaaaacaa ggccaccgcg     3300

aagaaauuuu ucuacucaaa uaucaugaac uucuuuaaga aagaugacgu cagaacugau     3360

aagaauggcg agauuauaug gaaaaaggac gaacacauca gcaacauuaa gaaaguguua     3420

ucuuauccuc agguuaauau cgugaagaaa gucgaggagc agacaggggg auucuccaag     3480

gaaaguauuc uccccaaagg caacagcgau aagcugaucc caaggaaaac gaagaaguuu     3540

uacugggaca ccaaaaagua ugguggguuc gacucuccca uaguggccua cuccauccuu     3600

guuauugcug auaucgagaa aggcaagagu aaaaagcuga agacugugaa agcauuggua     3660

ggagucacaa uuauggaaaa gaugaccuuu gagcgggacc cuguggccuu ccucgagcga     3720

aaaggcuaua gaaaugugca agaagagaac aucauaaagc ugccaaaaua cucacuauuu     3780

aagcuggaaa acgggcgcaa gaggcuucuc gcuagcgccc gugagcugca gaaaggaaau     3840

gaaauugucu ugccgaacca ucugggcaca uuacucuauc acgcaaagaa uauccauaaa     3900

guugaugagc ccaagcaccu ggacuacgug gauaaacaca aggacgaguu caaggaacuu     3960

cuggauguag ugucgaacuu uuccaaaaag uacacuuugg cggaggguaa ucucgagaaa     4020

aucaaggaac uguaugccca gaacaauggg gaagaccuaa aagagcuggc uucuagcuuc     4080

auuaaccuuc ucaccuuuac ggccauagga gcuccugcaa ccuucaaguu uuucgauaaa     4140

aauaucgacc ggaagagaua cacaucaacu accgagauuc ugaacgccac auugauccau     4200

caaaguauua cuggcuuaua ugaaaccagg aucgaucuga auaagcucgg uggcgacaaa     4260

cgcccagcag ccacgaagaa agcugggcag gcgaagaaaa agaaguaagg acuaguaaua     4320

aggaaacugu augaaugucu gugggcagga agugaagaga uccuucugua aagguuuuug     4380

gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua aaucugguag aaaaaugaga     4440

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaagaa uu                                                         4512


<210>  3627
<211>  3645
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3627
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcacagac aacaaaccug      240

agcuacauuc ucggacuuga ccuggguaua gccuccguug gcugggcggu gguagagauc      300

aaugaaaacg aggauccaau uggguugauc gacgugggag ucaggauuuu cgaacgggcu      360

gaggugccga agacuggcga aucuuuagcc cugucacgcc gacuagcacg uaguacgaga      420

aggcucaucc ggcgcagagc ccauaggcug cuucuggcua aacgguuuuu gaagcgcgag      480

gguauacuca gcaccaucga ucuggagaag gggcugccca aucaagcaug ggaacuccga      540

guugccggau uagagagaag gcuguccgcu auugaauggg gcgccgugcu uuugcaccug      600

aucaaacauc ggggcuaucu aucuaagcgu aaaaacgagu cgcagacaaa uaacaaggag      660

cucggggcgc ugcuuucagg agucgcacag aaucaccagc uguugcaaag cgacgauuac      720

cgcacuccug ccgaacucgc ucugaaaaag uucgccaagg aggaagguca uauuagaaac      780

cagaggggcg cauauaccca cacauuuaau cgguuagacc ugcucgcuga gcuuaaccug      840

uuguucgccc agcagcacca auuugggaau ccacauugca aagaacacau ccagcaguac      900

augaccgagc uacugaugug gcagaagccc gcgcucagug gagaggccau acugaaaaug      960

cuuggcaagu guacucauga gaaaaacgag uucaaggcag cuaaacacac auauuccgcc     1020

gaacgauuug uguggcugac caaguugaau aaccuccgca uucuggagga uggugcugaa     1080

agagccuuaa augaggagga aaggcaacug cuaaucaacc acccuuacga gaagucuaaa     1140

cucacguaug cacagguacg gaagcugcuu ggguugagcg aacaggccau uuucaaacau     1200

cugcgcuacu ccaaggagaa cgcugaauca gcgacuuuua uggagcucaa agcauggcac     1260

gccaucagaa aggcucugga gaaucaagga cuuaaggaca ccuggcagga ucuggccaaa     1320

aagccagacu ugcucgauga aaucggcaca gcauucaguc uguacaaaac ugacgaggau     1380

auucagcagu auuuaaccaa caagguuccc aauagcguga uaaacgcccu gcuagucucu     1440

cucaauuuug acaaauucau cgaacugucc cuuaagucau ugaggaagau ucugccucuc     1500

auggagcaag gcaaacguua cgaccaggcu ugccgggaga ucuaugggca ucacuacgga     1560

gaagccaacc agaagacaag ccagcuguua ccagcaauac cggcgcaaga gauucgaaau     1620

cccguggugc ugagaacccu uagucaggcu aggaaaguca ucaacgccau cauucgccag     1680

uaugguucuc cugcacgggu ucauaucgaa acgggcagag aguuggggaa guccuuuaaa     1740

gaaaggcgcg agauucagaa gcaacaggag gauaaucgaa cuaagcggga aagcgccgug     1800

cagaaauuca aggaacucuu cucggacuuu ucuuccgaac caaaaucaaa ggauauacug     1860

aaauuccguc uauacgagca gcaacacgga aagugucugu auaguggcaa agaaaucaac     1920

auucacagac ucaaugagaa ggguuacgua gagaucgacc augcucugcc cuuuagcagg     1980

acaugggaug acuccuucaa caauaaggug cuugucuugg ccucugaaaa ccagaauaaa     2040

gggaaccaga ccccuuacga guggcugcaa ggcaagauca acagcgaacg cuggaaaaau     2100

uuuguggcau uaguucucgg aucacagugc agugcugcca agaaacagcg gcugcuuacu     2160

caggugauug augacaacaa guucauagau agaaaucuga acgacacaag guauaucgcg     2220

cgauuuuugu ccaauuacau ucaagagaac cuccugcuag ucggcaagaa uaaaaagaac     2280

guguucaccc caaaugggca gaucacggcc cugcuccgca gccggugggg acugauuaaa     2340

gcuagagaaa acaauaacag gcaccaugca cuugaugcca ucguaguugc uugugccacc     2400

cccucuaugc agcagaagau aacacguuuu auccgcuuca aagaggugca cccguauaag     2460

auugagaauc gguacgaaau ggucgaccaa gagucaggug aaaucauuag uccucacuuu     2520

cccgagccau gggcauauuu cagacaggag gugaacauca gggucuuuga caaucauccu     2580

gauacugugu ugaaggaaau gcugcccgac cgaccacagg cgaaccacca guucguucaa     2640

cccuuauucg ugagccgggc cccuacccgc aaaauguccg gccaggggca uauggagaca     2700

auaaagucug cuaaaagacu cgccgaagga auuucgguac ugaggauccc acuuacucag     2760

cugaagccga accuacucga gaauaugguc aacaaagaac gggagcccgc acuguacgcu     2820

ggcuugaagg cccgacuggc agaguuuaau caagauccug ccaaggcuuu cgcgacccca     2880

uuuuauaaac agggugggca acaggugaag gccauuagag uugaacaggu gcagaaaagc     2940

ggcgucuuag ugcgcgagaa caauggagua gcagacaacg cuuccaucgu gaggacagau     3000

guuuucauaa agaauaacaa auuuuuccuc guccccaucu acaccuggca aguggccaag     3060

ggcauucugc cuaauaaagc caucguggcu cacaagaacg aagacgagug ggaagagaug     3120

gaugaggggg caaaguuuaa auucucacuu uuuccaaaug accuggucga auugaagacu     3180

aaaaaggagu acuucuuugg auauuacauu ggucucgauc gugccacggg caacaucagu     3240

cugaaagaac augacgggga gauaucuaag ggaaaggaug gcguuuaucg ggugggugug     3300

aaacuagcgc ugagcuucga aaaguaccag guagacgagc uugggaaaaa uagacagauu     3360

ugcaggcccc agcagcgcca gccgguccga aagagaccug cugccaccaa aaaggcaggc     3420

caagccaaga aaaagaaaua aggacuagua auaaggaaac uguaugaaug ucugugggca     3480

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     3540

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3645


<210>  3628
<211>  3723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3628
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagccgc guucaagcca      240

aacccgauua auuacauacu gggacucgac aucgguauug cuagcguugg cugggccaug      300

guggagaucg augaagacga gaaccccauu ugccuuaucg aucugggggu aaggguguuu      360

gaacgggcag aggucccuaa aaccggagac uccuuggcca uggcucgccg auuagcacgu      420

ucugugagaa ggcugacacg gcgcagagcc cauaggcuac uccgggcucg ccgacugcuu      480

aagagagaag gcguucugca ggccgcggau uucgacgaga augguuugau aaagucacuc      540

ccaaacacuc ccuggcaacu gagggcagcc gcucuggauc ggaaacucac gccuuuagag      600

uggagugccg ugcugcuuca cuugaucaag caucgugggu aucugagcca gcgcaaaaau      660

gaaggagaga ccgcagacaa ggaacuaggc gcucuccuga aaggcgucgc cgauaacgcg      720

cacgcccuuc agacagggga cuuuagaacu ccagcagagc uggcuuugaa uaaguucgag      780

aaggaauccg gacauauuag gaaccagcgg ggugauuacu cucacaccuu uucgcgaaaa      840

gaccuccaag ccgagcugau cuuacuguuc gaaaagcaga aagaguuugg caauccccac      900

gugucagggg gacucaagga aggcauugag acacuucuga ugacccagcg cccugcuuug      960

agcggugaug ccguacagaa aaugcuaggg cauuguacuu ucgagccagc agaaccgaag     1020

gccgcuaaaa acacauauac cgcggagaga uuuaucuggc ugacgaagcu caauaaccug     1080

aggauacuug aacaaggaag ugagcggccc cugacugaca ccgaacgcgc aacauugaug     1140

gacgagccuu acagaaaguc caaacucacu uaugcccagg cuaggaagcu guuaggccug     1200

gaggauaccg ccuucuuuaa aggccuacgu uacgggaagg acaaugcaga agccucuaca     1260

cucauggaga ugaaggcuua ucacgccauu agccgggcac uggaaaagga gggacuuaag     1320

gauaaaaagu ccccauugaa ccugucaccc gaacuccagg acgagaucgg uaccgcguuc     1380

agucuguuua aaacggauga ggacauuacu ggccgacuua aggauagaau ccagccugaa     1440

auccuggagg cuuugcucaa acauauuagc uucgacaagu ucguucaaau aucucugaag     1500

gccuuaaggc gcaucgugcc acugauggaa caggggaaac gguacgauga ggcaugcgcc     1560

gagauuuacg gagaccacua uggcaagaaa aauacagaag agaagaucua ccuacccccg     1620

auaccugcug augaaauuag aaaccccguc gugcucaggg cccuguccca ggcacgcaaa     1680

gugaucaacg gugucguucg acgguauggg ucaccagcuc guauccacau ugagaccgcc     1740

agagaagugg gcaagagcuu uaaggacagg aaagagaucg agaagcgcca agaagagaau     1800

cggaaagaca gagaaaaggc ggccgcuaaa uucagggagu acuuuccuaa cuucguagga     1860

gaacccaaga guaaagauau ucuuaaguug cgacuguaug agcagcagca uggcaagugu     1920

cucuacucug ggaaagagau aaaucuggga cgcuuaaacg aaaaggguua uguggagauc     1980

gaccacgcac ugccauuuuc ccggacuugg gaugacagcu ucaauaacaa aguccuugug     2040

uugggcucgg aaaaucagaa caaggggaau caaacacccu acgaguacuu uaacggaaaa     2100

gauaauucua gagaauggca ggaguucaag gccaggguug agaccucccg uuuuccucgc     2160

ucaaagaaac agcggauucu ccugcagaag uucgacgaag auggcuuuaa agagagaaac     2220

cuaaaugaca cgagguaugu gaaccgauuc cugugccaau uugucgcuga ucggaugcgc     2280

cucaccggua aggggaaaaa gagaguguuc gccaguaaug gccagaucac aaaccugcuu     2340

aggggauucu ggggcuugcg gaagguacga gcagaaaacg acagacauca cgcgcuggau     2400

gccguugugg ucgcuuguag cacuguggcc augcagcaga aaaucacccg cuuugucagg     2460

uacaaggaga ugaaugcauu cgacgggaaa acaauugaua aggagacugg agaaguguua     2520

caucagaaaa cccacuuucc acagccgugg gaguucuuug cucaggaagu uaugauacgu     2580

guguucggua agcccgacgg caagccugag uuugaagagg ccgacacacc agagaaacuc     2640

cggacccugc uugcagaaaa gcuguccucu agacccgagg ccguacacga auaugucacu     2700

ccuuuguucg ugagcagggc uccaaaccgc aaaaugucag ggcagggaca uauggagacg     2760

guuaagagug cgaaacgacu cgaugaaggc guguccgucc ugagagugcc ccuaacacaa     2820

cugaagcuca aagaccugga gaagauggua aaucgggaga gggaaccgaa acuuuacgag     2880

gccuugaaag cacgccugga agcucacaag gaugacccug ccaaagccuu ugcugagcca     2940

uucuauaagu acgauaaagc agguaaccgu acccaacagg ugaaggccgu uagagucgaa     3000

caagugcaga agacuggggu guggguccgg aaucauaacg gcaucgcgga caaugcuacc     3060

augguuaggg uggauguguu ugagaaagga gacaaguauu acuuaguacc cauuuacagc     3120

uggcaggucg ccaaaggcau ccucccugau cgcgcagugg uucaggggaa ggacgaggaa     3180

gauuggcaac ugauugacga uucuuucaac uuuaaauucu cacuucaccc caaugaccug     3240

guggagguca ucacaaagaa ggcccgaaug uucggauauu uugcuaguug ccacagaggc     3300

accgguaaca uaaauauccg gauucaugac cuagaucaca aaaucgggaa gaacggcauu     3360

cucgaaggaa ucggugugaa aacugcacug agcuuccaga aguaccagau agacgaguug     3420

ggcaaagaga uuaggccaug ucgccugaag aagagaccuc ccguuaggaa acggccagcc     3480

gcgacaaaga aagcugggca ggccaagaaa aagaaguaag gacuaguaau aaggaaacug     3540

uaugaauguc ugugggcagg aagugaagag auccuucugu aaagguuuuu ggaauuaugu     3600

cugcugaaua auaaacuuuu uugaaauaau aaaucuggua gaaaaaugag aucuaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     3720

auu                                                                   3723


<210>  3629
<211>  3729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3629
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggca      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagua ccacguugga      240

auugacgugg guaccuucag cguaggccug gccgcgauag agguggauga cgcugggaug      300

ccaaucaaaa cacucucccu ugucucucac auucaugauu caggacugga cccggaugaa      360

aucaagagug ccgugacuag guuggcaagc uccggcauug cccggcgcac gcgacguuua      420

uauagaagga agcggcgcag acugcagcaa cuagacaaau uuauccagag gcaggguugg      480

cccguuauag agcucgaaga uuacucugac ccucuguauc cauggaaggu gcgggcugag      540

cuugcagccu cguacaucgc ugaugaaaaa gagcgcgggg agaagcuguc agucgccuug      600

cgacacauug cgagacauag gggauggcgg aaccccuaug caaaagugag cagucucuac      660

cugccugacg gcccauccga ugccuucaag gcuauccgug aagagauuaa gcgcgccucu      720

ggccagcccg uaccugaaac cgcaacaguu gggcaaaugg ugacucugug cgagcucgga      780

accuuaaaac ugagagguga gggcgggguc cuuagcgcua gguugcagca guccgacuau      840

gcccgggaaa uccaggagau augucgaaug caagaaauug gacaggagcu guaccgcaag      900

aucauugaug ugguguuugc ggccgaauca ccaaaaggca gugcaagcuc uagagucggu      960

aaggacccgc uacagcccgg gaaaaauagg gcucucaagg ccuccgacgc uuuccagcgg     1020

uaccgcaucg ccgcacugau cggaaaccuu agaguuaggg uggauggcga gaaacguauu     1080

cugucaguag aggaaaagaa uuugguguuu gaccaccucg ucaaccugac accuaagaaa     1140

gagccagaau gggugaccau agccgagauc uuaggcauug aucgggggca acugaucgga     1200

acugcuacaa ugaccgacga uggugaacga gcgggcgcaa gacccccuac gcacgacacu     1260

aauaggagca uaguuaacag ucgcauugcc ccacucgugg auugguggaa gaccgcuucu     1320

gcccuugagc agcaugcaau ggucaaagcc cuguccaaug cugaggugga cgauuucgac     1380

agccccgaag gggccaaggu acaggcauuu uucgcggauu uggacgacga uguucacgcu     1440

aaacuagacu cgcugcaucu cccgguggga cgggccgcau auucugagga uacacugguc     1500

agacuuacua ggcgcaugcu guccgacggc guggauuugu acaccgcccg acuccaagaa     1560

uuugguaucg agccuucaug gacaccccca accccucgga ucggggaacc cgucggcaac     1620

ccagcugugg accguguucu gaagacggug aguagauggu uagagagcgc cacuaagaca     1680

uggggagcac ccgagagggu aauuaucgaa cacguccgcg agggcuucgu gaccgaaaaa     1740

cgggcuagag agauggaugg ggacaugagg cgacgcgccg cgcggaaugc caagcuguuu     1800

caggagaugc aggaaaaacu aaacguucag ggaaagccuu ccagagcuga ucucuggagg     1860

uaucaaucug ugcagcguca gaauugccag ugugcauacu gcgguagccc aauuacuuuc     1920

ucaaacagug agauggacca cauagucccg cgcgccggcc aaggguccac aaauacccgg     1980

gaaaaccugg uggcuguaug ucauagaugc aaucagagca aaggaaacac gcccuuugcc     2040

aucugggcaa agaacaccuc uauugagggc gugucaguua aggaagcggu cgagaggaca     2100

cgacacuggg ugacugauac cgguaugcgg aguacagacu ucaaaaaguu cacuaaagcc     2160

guggucgagc gcuuucagag agcuaccaug gaugaagaga ucgacgccag gagcauggaa     2220

uccguugcau ggauggcuaa ugagcuucgg ucucgagugg cccagcauuu cgcaucgcac     2280

gggacaaccg ugagaguaua ucgcggcagc uugacugccg aagcuaggcg ugcguccgga     2340

aucucaggca agcugaaauu uuucgacggg gucggaaaga gucggcucga uagaaggcau     2400

cacgccauug acgcagcugu gauagccuuu acgucugauu acguugccga gacacuggcu     2460

gugcgcagca accuuaagca aucccaggca caccgacagg aggccccuca guggagagag     2520

uucaccggua aagacgcgga acaucgggcu gccuggaggg ucugguguca aaagauggaa     2580

aaacugucag cauugcucac ugaggaucug cgcgacgauc gugugguugu gaugagcaau     2640

guaagauuac ggcugggcaa cgggucugcc cacaaggaaa ccaucggaaa acuauccaag     2700

gucaaacuca guagccagcu gucugugucc gacauugaua aggcuucaag ugaggcacuu     2760

uggugcgccu ugacaaggga gccaggcuuu gaccccaagg aaggucugcc ugcgaaucca     2820

gagcgccaua uccgagugaa cgggacccac gucuaugcug gcgauaauau uggacucuuc     2880

cccguuagcg ccggcucgau cgcacugaga gggggauacg ccgaauuagg cuccucuuuu     2940

caccaugcuc ggguguacaa aauaacuagc gguaagaaac cggccuucgc aaugcugagg     3000

guauauacaa ucgaccuuuu gccuuaccgc aaccaggauc ucuuuucagu ggagcugaag     3060

ccacaaacga ugaguaugag acaggcugag aaaaagcuaa gggacgcccu ggcgaccggg     3120

aaugccgaau aucucggaug gcuggucgug gacgaugagc uuguugugga cacuuccaag     3180

auugcaacag aucaggucaa agcuguggaa gccgaguugg gcaccauccg gcguuggcgc     3240

guugacgguu ucuuuucucc cagcaagcug cgauuaagac cucuccagau guccaaagaa     3300

ggcauuaaga aagagucagc ucccgagcug agcaagauca uagauaggcc aggguggcuu     3360

ccugcaguga acaagcuguu cagugacgga aaugucacag uagugcggcg cgauucuuug     3420

ggcagaguua ggcucgaauc cacugcccau cugcccguga ccuggaaagu ccaaaagcgg     3480

ccagccgcua cgaaaaaggc ggggcaggcc aaaaagaaga aauaaggacu aguaauaagg     3540

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa     3600

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaagaauu                                                             3729


<210>  3630
<211>  3429
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3630
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagccag gauucuggcg      240

uucgacauag gaaucagcuc cauugguugg gcuuuuucug agaacgauga acucaaggac      300

ugcggcguuc ggaucuucac caaaguggag aauccaaaga caggggaauc acuugcccug      360

ccgcgccgau uggcacguag ugccagaaag agguuagcuc ggcgcaaagc aagacugaac      420

caucuaaagc accucauugc caaugaguuu aaacugaacu acgaagauua ucagagcuuc      480

gacgaguccc uugcuaaggc cuacaaagga ucucugaucu cgcccuauga guugagguuu      540

cgggcgcuca augaacugcu gucaaagcaa gauuucgcac gcguaauacu ccauaucgcc      600

aagcgaagag gcuacgacga uauuaaaaac agcgacgaua aggagaaagg ugcuaucuua      660

aaggccauua aacagaauga agagaagcug gcaaacuauc agaguguggg ggaguaccuu      720

uauaaagaau acuuucagaa guucaaggaa aauuccaaag aauuuacuaa cgucaggaau      780

aagaaagagu cuuacgaacg guguaucgcu cagagcuucu ugaaggacga gcugaaacua      840

auauuuaaga agcaacguga guucggauuu uccuucucua aaaaguucga agaggaagug      900

cucaguguug ccuuuuauaa acgcgcgcug aaggauuuca gccaccuugu gggcaacugc      960

ucauuuuuca cggacgagaa aagagccccu aaaaauuccc cacuggcauu uauguucguc     1020

gcuuugacca ggauuaucaa ccuccugaau aacuuaaaga acacagaagg cauucuguac     1080

acuaaagaug accucaaugc ccuucugaac gagguguuga agaaugggac ccuaacauau     1140

aaacagacca agaaacugcu cggacuguca gacgauuacg aguuuaaggg ugaaaagggc     1200

acuuauuuca ucgaguuuaa aaaguacaaa gaguucauca aggcucuugg ggagcauaac     1260

cugagccagg acgauuugaa ugaaauugcc aaagacauaa cacucaucaa ggaugagauu     1320

aaacugaaga aggcauuagc caaauaugac cugaaccaaa aucagaucga uagucuaucu     1380

aagcucgagu uuaaagacca ccugaacaua uccuucaagg cucuuaaauu gguaacuccc     1440

cugaugcucg aaggaaagaa guacgaugag gcguguaaug aacugaaccu uaaaguugca     1500

auuaaugagg acaagaaaga uuuucugccu gccuucaacg agacguacua uaaggacgaa     1560

gugacuaauc cagucguguu gcgggcuauc aaagaguacc gaaaggugcu caacgcccug     1620

uuaaagaaau auggcaaggu ccacaaaauc aauauugaac uggcacgcga gguugguaag     1680

aaccauagcc agagagccaa aaucgaaaag gagcagaacg agaauuacaa ggcuaaaaag     1740

gacgccgaac uagagugcga aaaacucggg cugaagauua acucgaaaaa uauacuuaag     1800

uugaggcugu ucaaagagca aaaggaauuu ugugcauauu cuggagagaa gaucaaaauu     1860

uccgaucucc aggacgagaa gaugcuggaa aucgaucaca ucuaccccua uucacggagu     1920

uucgacgaua gcuacaugaa caaaguguua guauuuacca agcagaauca agagaaacug     1980

aaccagacac cuuucgaagc guuuggcaau gacuccgcua aguggcagaa gauugaggug     2040

cuugccaaaa acuugccaac uaagaaacag aagcgcauac ucgauaaaaa uuacaaggac     2100

aaggaacaaa agaacuucaa ggauagaaau cugaacgaca ccagguauau cgcacgucua     2160

guccugaauu acacaaaaga uuaucucgac uuucugccgc uuucugauga cgagaacacc     2220

aaguugaaug acacgcagaa aggcagcaag gugcauguug aggccaaguc agggaugcug     2280

accagugcuu uacggcacac auggggauuc uccgccaaag aucgaaacaa ccaccuccau     2340

cacgcaauug acgcugugau cauugccuac gcgaauaaca gcaucgucaa ggccuuuucu     2400

gauuucaaaa aggaacagga gucaaauagu gcugaacugu augccaaaaa gauaagcgag     2460

cuugacuaca agaacaaaag aaaguuuuuc gagcccuuuu ccgguuucag gcagaaagug     2520

cuggauaaga ucgacgaaau uuucguaucu aaaccugagc gcaagaaacc aucgggcgca     2580

uugcaugaag agaccuuucg gaaggaagag gaguucuauc aaagcuacgg gggaaaggaa     2640

ggcguucuca aagcucugga gcuagguaag aucagaaaag ugaaugggaa gauugucaaa     2700

aacggcgaua uguuuagggu ggacaucuuc aagcacaaga aaacuaauaa guuuuacgcc     2760

guccccauau auacaaugga uuucgcacug aaagugcucc cuaacaaggc gguugcccgc     2820

uccaaaaagg gagaaauuaa ggacuggauc cugauggaug agaauuacga auuuugcuuc     2880

ucacuuuaua aagacaguuu gauucugauc cagaccaagg auaugcagga gccagaguuu     2940

guguacuaua acgcuuucac gucuagcacc guauccuuaa uagucucaaa acaugacaau     3000

aaguuugaaa cacucagcaa aaaccagaag auccuguuca agaaugccaa cgagaaagaa     3060

gugauugcaa agucuaucgg cauucaaaau cuuaaaguuu ucgagaagua caucgugucc     3120

gcucuggggg aagucacuaa agccgaguuu cgacagcggg aggacuucaa gaaaaagcgu     3180

cccgcagcca ccaagaaagc uggacaggcg aagaaaaaga aauaaggacu aguaauaagg     3240

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa     3300

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu     3360

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3420

aaaagaauu                                                             3429


<210>  3631
<211>  3840
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3631
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaagcga ccugguucuc      240

ggacuugaua uugguauagg cuccgugggg guaggaaucc ugaacaaggu gaccggcgag      300

auuauccaua aaaauucuag gauuuuccca gccgcgcagg cugaaaacaa uuugguccgg      360

cgcacaaacc gacaaggucg uagauuagcc aggcggaaga agcaccgcag agugaggcug      420

aaucggcuau uugaggaauc agggcucauc acugacuuca cgaaaauaag uaucaaccug      480

aauccguacc agcuucgcgu uaagggacug accgaugagu ugagcaacga agaacucuuu      540

auugcacuga aaaauauggu gaagcaucga ggcaucuccu aucuggacga ugccucugac      600

gauggcaacu cgucagucgg ggacuacgcu cagauuguga aagagaauag caagcagcuc      660

gaaacaaaga cucccggaca aauccaguua gagagauauc agaccuacgg ucagcugagg      720

ggcgauuuca caguagaaaa agacgggaag aaacaccggc uuauaaacgu uuuuccuacc      780

agugcauauc guuccgaggc cuugcgcauu cugcaaacuc agcaggaguu caauccacag      840

aucacagaug aauuuauuaa cagauaccua gagauccuca ccggaaagag gaaauauuac      900

cauggccccg guaaugaaaa gucucggacg gacuacgggc gauaucgcac uagcggagag      960

acccuggaua acaucuucgg cauucuuaua ggcaaaugca cauuuuaccc ugacgaguuc     1020

agagcugcca aggcguccua uacugcacaa gaguuuaacc uguugaauga ccucaacaau     1080

cugaccgugc caacagagac caagaaauua ucaaaggaac agaaaaacca gaucauuaau     1140

uacgucaaga acgagaaagc cauggggccc gcuaagcugu ucaaguauau cgccaaacuc     1200

cuuaguugug auguggcaga cauaaaggga uacaggauug auaaaagcgg uaaggcugaa     1260

auccacacgu uugaggccua ucggaaaaug aagacucugg aaacauugga caucgagcaa     1320

auggaucgcg agacccuaga caagcuggcg uacgugcuca cucugaauac agaaagagag     1380

ggcauucagg aagcccuuga gcacgaguuc gcagaugggu cuuucuccca gaaacagguc     1440

gacgagcugg uucaauuuag gaaggcuaac ucaagcaucu ucggaaaagg cuggcauaau     1500

uuuaguguga aguugaugau ggagcucauu ccugaacugu acgagaccuc ugaagaacag     1560

augacgauau uaacccgucu ggguaaacag aagacaacuu ccagcucgaa caagaccaaa     1620

uauaucgaug agaagcuacu cacagaagag auuuacaauc caguaguggc caaaucuguc     1680

cggcaggcua ucaagaucgu gaacgccgca auuaaagaau auggggacuu cgauaauaua     1740

guuaucgaga uggcccgaga aacuaacgag gacgacgaga agaaagcuau ucaaaagauc     1800

cagaaggcga auaaagauga aaaggacgca gccaugcuga aagcugccaa ccaguacaac     1860

ggcaaggcag agcuuccgca cuccguguuu cauggacaca aacaguuggc caccaagauu     1920

agacuguggc accaacaggg cgaaaggugc cucuauacag ggaagaccau cucaauacau     1980

gaucugauca auaacaguaa ucaguucgag gucgaccaca uucuuccccu gagcaucacu     2040

uuugaugacu ccuuggcuaa caaagugcuc guauacgcca cggcaaauca ggaaaaggga     2100

caacgcacac cuuaucaggc gcuggauucu auggacgaug cuuggagcuu ccgggaguua     2160

aaagccuuug uuagagaguc aaagacccug aguaacaaaa agaaggaaua ccuacucacu     2220

gaggaagaca uuuccaaauu cgaugugagg aagaaauuua ucgagcgcaa ucuggucgac     2280

acccgauacg caagccgggu gguccuuaac gccuugcagg aacauuuccg ugcucacaag     2340

auagauacaa aagugucugu ugugagaggu caauuuaccu cacagcugag gcgccauugg     2400

ggcauugaga agacucggga cacauaucac caccaugccg uagacgcacu caucauugcu     2460

gccaguagcc agcugaauuu auggaagaaa cagaagaaca cgcuggucuc cuacucugag     2520

gaucaacuuu uggacaucga aaccggggag cucauaucgg augacgaaua uaaagagagc     2580

guguucaagg cgccauacca gcacuucguu gauacucuga aauccaagga guuugaagac     2640

ucaauccuau ucaguuauca gguggauucu aaauuuaaua gaaagauuag cgacgccaca     2700

aucuacgcua ccaggcaggc aaaggucgga aaagauaagg ccgacgagac auaugugcug     2760

ggcaaaauua aggauaucua cacucaagac gguuacgaug cuuucaugaa aauauauaag     2820

aaggacaaau ccaaguuucu cauguaccga caugaccccc agaccuucga aaaaguaauu     2880

gagccuaucc uggaaaacua uccaaauaag cagaucaacg agaaagggaa ggaggugccc     2940

uguaauccgu uucuuaagua caaagaggaa cacggcuaua uucgcaagua cagcaaaaag     3000

ggaaacggcc cugaaaucaa aucauugaag uauuacgauu cuaagcuggg gaaccacauu     3060

gacauaacgc ccaaagauuc caauaacaag guugucuuac agagugugag cccauggcgg     3120

gccgacgugu acuucaauaa aaccacagga aaguaugaga uccucggucu gaaauacgca     3180

gaucuucaau uugaaaaggg cacugggacc uauaagauuu cucaggagaa auacaacgac     3240

aucaagaaaa aggagggagu cgauuccgac ucagaguuca aauuuacacu guauaagaau     3300

gauuugcucc ugguuaaaga cacugaaacc aaggaacagc aacuauucag auuucugagu     3360

aggacgaugc cuaagcagaa acauuacgug gagcucaagc ccuaugauaa acagaaguuc     3420

gaaggcggug aggcgcugau uaaagugcuu gggaacguag ccaauagcgg ccagugcaag     3480

aagggauugg gcaaaucgaa caucuccaua uacaaggucc guacagacgu gcuggggaau     3540

caacacauca uuaaaaacga gggagacaag ccaaaauuag auuucaagcg ccccgcugcc     3600

accaagaaag caggccaggc uaagaaaaag aaauaaggac uaguaauaag gaaacuguau     3660

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     3720

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     3780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3840


<210>  3632
<211>  4398
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3632
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaccca guucgaggga      240

uuuacaaacc uguaccaagu uagcaagacu cucagguucg aacuuauucc acaggguaaa      300

acgcugaagc auauacagga gcagggcuuu aucgaagagg acaaggcccg gaaugaucac      360

uauaaagaau ugaagccgau uaucgaccgc auuuacaaaa ccuaugcgga ucaaugcuua      420

cagcuggugc agcuagacug ggagaaccuc uccgcugcca ucgauucuua ccgaaaggag      480

aaaacagaag agacucguaa ugcacugaua gaggaacagg ccaccuauag aaacgcuauc      540

caugacuacu ucauugggag gacagauaau cuuaccgacg caaucaacaa gcggcacgcc      600

gagauuuaua agggacuguu uaaagcugaa uuguucaaug gcaagguacu caaacaacug      660

gguacuguga caaccacgga gcaugaaaac gcccugcucc gcucauuuga uaaguucacu      720

accuacuuua guggguucua cgagaauagg aaaaacgucu uuagcgcgga agacaucucc      780

acagcaauac cccacagaau ugugcaggau aauuucccua aguuuaaaga gaacugucac      840

aucuucacuc gguuaauuac cgccguucca ucucugcgcg agcauuucga aaaugugaag      900

aaggcuaucg gaaucuuugu cucgacauca auugaggaag uguucagcuu ucccuucuau      960

aaccagcuuu ugacccagac gcaaauagac cuguacaauc agcuacucgg cggcaucagu     1020

cgagaggccg ggacugagaa aauuaaggga cugaacgaag uacuuaaccu ggcaauccag     1080

aaaaaugaug agacagcuca cauaauugcc uccuugccuc auagauuuau cccacucuuc     1140

aagcaaaucc ugucugacag gaacacccuu agcuuuauuc uggaagaguu caaauccgac     1200

gaagagguua uccagucauu uugcaaguau aagacucucc uucggaauga aaacgugcug     1260

gagacagcgg aggccuuguu caaugaacua aacaguauug aucugaccca cauauuuauc     1320

agccacaaaa agcucgagac gauuucuucc gcacugugug accauuggga uacccuucgu     1380

aaugcucugu acgaacgcag aaucucagag uugacaggua aaaucacuaa gagcgccaaa     1440

gagaaggucc agaggagucu caagcacgaa gacauuaacc ugcaggagau aaucucugcu     1500

gccggcaaag aauuauccga ggcauucaag cagaaaacca gcgaaauucu gucgcaugcc     1560

cacgcugcgc uagaucaacc ccucccuaca acucugaaga aacaggagga gaaggaaauc     1620

cuuaagucuc aguuggacuc ccugcucggg cuguaucauc uucuggauug guuugcagug     1680

gacgagucaa augaagugga uccagaguuc agugcccggu ugaccggaau uaaacucgaa     1740

auggagccga gccuguccuu cuacaacaag gcucgaaauu augccacuaa aaagcccuac     1800

ucugucgaga aauuuaaguu aaacuuccaa augccuaccc uggcaagcgg cugggacguu     1860

aauaaagaaa agaacaaugg ugccauccua uuugugaaga acgggcucua cuaucuggga     1920

auaaugccaa aacagaaggg ccgcuacaaa gcucuuucau ucgagcccac ugaaaagacg     1980

agugagggcu uugauaaaau guauuacgac uauuucccug acgccgcaaa gaugauccca     2040

aagugcucca cacaguugaa agcgguaacc gcucacuuuc agacucacac cacacccauu     2100

cugcucagca acaauuucau cgaaccgcug gagauuacca aggagaucua cgauuuaaac     2160

aauccugaaa aagagcccaa gaaauuucaa acugccuaug caaagaagac aggggaccag     2220

aaaggauaca gagaagcccu guguaagugg auagauuuca cgagggacuu ucuuucuaaa     2280

uacaccaaga cuacaucaau ugauuugagu agccuccggc cauccucuca guauaaagac     2340

cugggugagu acuaugcuga gcuaaacccu cugcucuacc auaucucguu ccagcgcauu     2400

gccgaaaagg agaucaugga ugcaguggaa accggcaagc uguaucuuuu ucaaauauac     2460

aauaaagacu ucgcuaaggg gcaccaugga aaacccaacu ugcacacacu guauuggacu     2520

ggcuuauuca gcccagagaa ucucgccaag accuccauca aacugaacgg ucaggcggaa     2580

uuauuuuaca gacccaaguc aaggaugaaa cguauggccc accggcuggg ggagaagaug     2640

uugaauaaga aacucaagga ucagaaaacg ccuauuccag acacccugua ccaagagcua     2700

uaugauuacg ucaaccaucg acugagucac gaccucucug augaagcuag agcacugcuu     2760

ccgaauguga ucacaaagga gguuagccau gaaauuauca aagacaggcg cuucacuucc     2820

gacaaguuuu ucuuucacgu gcccauaacc uugaacuauc aggccgcuaa uucaccuagc     2880

aaguucaacc agcgggucaa ugccuaccug aaagagcauc cagaaacacc cauuaucggc     2940

aucgauagag gagagaggaa cuuaauuuau aucacuguga uugacucuac cggcaagaua     3000

cucgagcagc gcucccugaa cacgauccaa caguuugauu accagaaaaa gcuggacaau     3060

cgagaaaaag agcggguagc agcgcgucag gccuggagug uuguggggac aauuaaggau     3120

cuuaagcaag gauaucuaag ccaggucauc cacgaaauug uggaccucau gauccacuac     3180

caggcugucg ugguucugga gaacuugaau uucgguuuua aaucuaagag aaccggcaua     3240

gccgaaaaag caguguacca gcaauucgag aagaugcuga ucgauaaauu aaacugccuc     3300

guacugaagg acuauccugc ugagaagguc gggggagugc uuaauccaua ccagcugacu     3360

gaucaguuua cauccuucgc caaaaugggc acccaaucag guuucuuguu uuauguuccc     3420

gcaccguaca caaguaagau ugacccucuc accggguucg uggauccauu ugucuggaaa     3480

acaaucaaga accaugaaag caggaaacac uuccuggagg gcuuugacuu ccuacauuau     3540

gaugugaaga cgggagacuu uauucugcac uucaagauga aucgcaaccu uucguuucag     3600

cggggccucc ccggguucau gccugccugg gacaucguau uugagaaaaa ugaaacccag     3660

uucgaugcua agggaacucc cuuuauagcg gguaaaagaa uugugccagu uaucgagaac     3720

cauagguuca caggccgaua ccgcgaccug uauccugcca augaauugau cgcacuguua     3780

gaggaaaagg ggauugucuu ccgggaugga uccaacauac uccccaaacu gcuugagaau     3840

gacgauucuc acgcuaucga caccauggug gcccugauua gaagcguguu gcagaugagg     3900

aacucaaaug ccgcuacagg cgaagauuac aucaacaguc caguccguga ccucaacggu     3960

guuuguuuug auucccgcuu ccaaaauccg gaguggccua uggacgcaga ugccaacggg     4020

gcguaccaca uugcucugaa gggccagcua cugcuuaauc aucucaaaga gucuaaggac     4080

cugaaguugc agaacggaau cagcaaucag gacugguuag ccuauauaca agaacugcgg     4140

aacaaaagac ccgcagccac uaagaaagcu ggccaggcaa agaaaaagaa guaaggacua     4200

guaauaagga aacuguauga augucugugg gcaggaagug aagagauccu ucuguaaagg     4260

uuuuuggaau uaugucugcu gaauaauaaa cuuuuuugaa auaauaaauc ugguagaaaa     4320

augagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaagaauu                                                   4398


<210>  3633
<211>  4377
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3633
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaagcau uuaccaggag      240

uucguuaaca aguauucccu gucuaaaacc cucagguuug aacuuauacc acaaggaaag      300

acacuggaga auaucaaggc ccgggguuug auuuuagacg augaaaaacg cgcgaaggac      360

uacaaaaagg cuaaacagau cauugauaag uaucaucagu ucuuuaucga ggaaauacug      420

ucaagugugu gcaucagcga ggaccuacuc cagaacuacu ccgauguaua uuucaagcug      480

aaaaagucug acgaugacaa ucuucaaaaa gauuuuaagu cggccaaaga cacuauuaag      540

aaacagaucu cagaguacau uaaggauagc gaaaaguuca aaaaccuguu uaaucagaac      600

uugaucgacg caaagaaagg ccaggagagu gaucucauac uguggcugaa gcaauccaaa      660

gacaauggga uugaacucuu caaggccaac ucugacauca cggauauuga cgaggcuuua      720

gagaucauca agagcuuuaa aggauggacc acauauuuca agggcuuuca cgaaaaucgu      780

aaaaacgugu acuccucaaa ugauauuccg acaaguauaa ucuaccgaau ugucgacgau      840

aaccugccca aguuccuuga gaauaaagca aaguaugaga gcuugaagga caaagccccu      900

gaggcuauca acuacgaaca gauaaagaaa gaucuggccg aggagcuaac cuuugacauu      960

gauuauaaga caucugaagu gaaucagaga guuuucuccc ucgacgaggu guucgaaauc     1020

gcgaacuuua acaauuaccu gaaccaguca gguaucacca aauucaauac uauuaucggg     1080

ggaaaguuug ucaacggcga gaauacaaag aggaaaggca uuaacgaaua uauaaaucuu     1140

uacagccaac agaucaacga uaagacccug aaaaaguaua aaaugagugu guuguucaag     1200

cagauucucu cugacacgga guccaagagc uuuguaaucg acaaacugga ggaugacucg     1260

gauguuguga cuaccaugca aucuuucuac gaacagaucg cagccuuuaa gacagucgag     1320

gaaaaaucca uuaaggagac uuuaucacuc cuguucgacg aucuuaaagc ucagaagcug     1380

gacuugagua aaauauacuu uaagaaugau aagagccuaa ccgaccuguc ccagcaagug     1440

uucgaugacu auucugugau cgggacagcc guccucgaau acauuaccca gcagaucgca     1500

cccaaaaacc uggauaaucc aagcaagaaa gagcaggagc uuauugcuaa gaaaacggaa     1560

aaggccaagu aucugucauu ggagacuauc aaacucgcgc uggaagaguu uaacaagcau     1620

cgggacauag auaaacaaug ucgcuucgag gaaaucuuag ccaauuuugc agcuauuccu     1680

augaucuucg acgagauugc ccagaacaag gacaaucugg cucagaucag uauaaaauac     1740

cagaaccaag gaaagaagga ucuacuccag gccuccgcag aagacgaugu uaaagccauu     1800

aaggaccugc uugaucagac aaacaauuug cugcacaaac ucaagaucuu ccauauuagc     1860

cagucugagg acaaagcuaa cauccuggau aaggacgaac acuuuuaucu uguguucgag     1920

gagugcuacu uugaacuggc gaauauagua ccauuguaua acaaaaucag aaauuacauu     1980

acccaaaagc ccuacucaga ugagaaguuc aaacucaacu uugaaaauag uacucuggca     2040

aacgguuggg acaagaauaa agagccugau aacacagcca ucuuauucau uaaggacgau     2100

aaauauuacc ugggcgugau gaauaagaag aacaauaaaa ucuuugacga caaggcuaua     2160

aaagaaaaca agggggaggg auauaaaaag auugucuaca agcuacuccc aggcgccaau     2220

aaaaugcugc cgaagguguu cuuuagcgca aaauccauca aguucuauaa ccccucugag     2280

gauauccuua ggauucggaa ccacucgacc cauacgaaaa augguagccc ucagaagggg     2340

uacgaaaagu uugaguucaa caucgaagac ugucgcaaau uuauugauuu cuauaagcag     2400

uccauaucaa aacacccaga guggaaggac uucggauuuc gauucaguga uacccaaaga     2460

uacaauucua ucgacgaauu uuacagggag guugagaacc agggcuauaa auugacauuc     2520

gaaaauauua gcgaguccua caucgauuca guggucaacc agggcaagcu guaucucuuu     2580

cagauuuaca auaaggacuu cagcgccuau ucuaaagggc ggcccaaccu gcauacuuua     2640

uacuggaagg cucuguuuga ugaacguaau cuucaagacg ugguauauaa auugaacgga     2700

gaggccgaac ucuucuaccg caagcagucc aucccuaaaa agauaaccca cccagcaaaa     2760

gaagcgaucg cuaauaagaa caaggauaau cccaaaaagg agaguguuuu ugaauacgac     2820

cugauuaaag acaagagauu cacagaggau aaauuuuucu uccacugccc gaucacuauu     2880

aacuuuaaga gcucuggugc caauaaguuc aacgacgaaa ucaaccuacu gcucaaagag     2940

aaggcaaaug augugcauau acuguccauu gacaggggcg agcggcaccu ugccuauuac     3000

accuuggucg augggaaagg aaacaucauc aagcaggaca cauuuaauau uauaggcaac     3060

gaucgaauga aaaccaauua ucaugacaag cuggcugcca ucgaaaagga ucgcgacuca     3120

gcaagaaaag auuggaagaa aauuaacaau aucaaggaga ugaaagaagg uuacuuaagu     3180

cagguggucc acgagauugc uaagcucgug aucgaauaua acgccauagu uguguucgag     3240

gaccugaauu uuggguucaa gaggggccgg uuuaaaguag agaagcaagu cuaccagaaa     3300

cuugaaaaga ugcugaucga gaaauugaac uaucucgugu ucaaggauaa ugaauuugac     3360

aaaacuggag gcguucugcg cgcguaccag cuaacggccc cuuucgagac auuuaagaag     3420

auggggaaac agaccggaau uaucuacuau gugcccgcug guuucacuag caagauuugu     3480

ccagucaccg gcuuugugaa ccaacuguac ccuaaauaug aaucgguauc caagucucag     3540

gaguucuuca gcaaauuuga caagaucugc uacaaucucg auaaggggua uuucgaguuu     3600

ucauucgacu acaaaaacuu uggagauaag gcagccaaag gcaaguggac aauagcuagu     3660

uucgguucca gacugauuaa uuuuaggaac ucugacaaaa aucauaacug ggauacccgu     3720

gaaguguauc ccacuaagga gcuugaaaag uugcugaaag acuacagcau cgaguacggg     3780

cacggcgagu guaucaaggc cgcaauuugc ggagaauccg auaaaaaguu cuuugcgaaa     3840

uuaacaucag uucucaacac gauccugcag augcggaaua gcaagaccgg cacugagcuu     3900

gacuaucuga uaaguccagu cgccgaugug aacgggaauu ucuuugacuc ucgacaagcu     3960

cccaagaaca ugccucagga ugccgacgca aauggagcuu accacauugg ucuaaaaggc     4020

cucaugcugu uggggagaau caagaacaau caggaaggca aaaagcugaa cuuagugauu     4080

aaaaaugagg aauauuucga guuuguccag aacaggaaua acaagcgccc agccgcaacu     4140

aaaaaggccg gacaagcuaa gaaaaagaaa uaaggacuag uaauaaggaa acuguaugaa     4200

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4260

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4377


<210>  3634
<211>  4517
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3634
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagacaa gaaauacagc      240

auuggacugg auauagguac aaacuccguu ggcugggccg ugaucacaga cgaguauaag      300

guaccaucua agaaauucaa ggugcucggg aauacugaua ggcauucaau uaaaaagaac      360

cuuaucggag cgcuguuguu ugacaguggc gaaacggcug aggccacccg guuaaaacgc      420

acagcacgac guagauacac uaggcggaag aaucgcauuu gcuaucugca ggaaaucuuc      480

agcaacgaga uggccaaggu cgaugacucc uuuuuccaca gacuagaaga gucuuuucuc      540

guggaggaag auaaaaagca ugagaggcac ccgauauucg guaauaucgu ugacgaagug      600

gcuuaccaug agaaauaucc caccauuuac caucugcgga agaaacuugu cgauucgaca      660

gacaaggcag aucugcgcuu gaucuaucuc gcccuggcuc acaugauuaa auuucgaggg      720

cacuuccuga ucgagggaga ccucaacccu gauaauucag acguggacaa guuauuuaua      780

caacugguac agaccuacaa ccagcuuuuc gaagagaauc caauuaacgc cagcggcguu      840

gaugcgaagg caaucuugag ugccagacug uccaaaucua ggcggcuaga aaaucucauu      900

gcucagcugc ccggcgagaa gaaaaacggg cuuuuuggaa aucugaucgc cuugagccuc      960

ggucugacuc cuaacuucaa guccaauuuu gacuuagcag aagaugcuaa acugcaacuc     1020

ucaaaggaca cauacgauga cgaucuugac aaccuguugg cccagaucgg cgaucaguau     1080

gcggaccuau uccuggccgc aaagaaccuc agugaugcua uucugcuuag cgacauacug     1140

cgugugaaua ccgagaucac gaaagcccca uugucugcuu ccaugauuaa gcgcuacgac     1200

gagcaccauc aggaucucac ucuguuaaaa gcccugguca gacaacagcu acccgaaaag     1260

uauaaagaga ucuucuuuga ccagucaaag aacggguacg caggauauau agauggcggu     1320

gccagccagg aagaguucua caaguuuauu aaaccuaucc ucgaaaagau ggacgggacc     1380

gaggagcugc uugugaaauu gaauagggaa gaucugcucc ggaagcaacg aacauucgac     1440

aacggaagua ucccacacca gauucaccug ggcgagcuuc augcuauccu gcgcagacag     1500

gaagauuuuu auccguucuu gaaagacaau agggagaaga uugaaaagau acucacuuuu     1560

cggauccccu acuacguggg cccucuggcg cgcgggaacu cuagauucgc auggaugacc     1620

aggaaauccg aggagacaau uaccccaugg aauuuugaag aggucguuga uaagggagcc     1680

agcgcucaau cguucaucga acguaugacg aacuuugaca aaaauuuacc caacgagaag     1740

gugcugccua aacacucucu acucuaugag uacuucacug uauauaauga acugacaaag     1800

gugaaauacg ucaccgaggg uaugcggaag ccagccuuuc uuuccggcga acagaagaaa     1860

gcaaucgugg auuugcuguu caagacuaac cgaaaaguua cagugaagca gcucaaagag     1920

gacuauuuca agaagauuga auguuuugau ucagucgaga uaaguggggu ggaggacaga     1980

uucaaugcca gccugggaac cuaccaugac uuacugaaaa ucauuaagga uaaagacuuu     2040

cuugauaacg aagagaauga agacaucuug gaggauauug uacucacgcu gacccuauuc     2100

gaagacaggg agaugaucga ggaacgccug aagacauaug cucaccucuu ugaugacaaa     2160

guuaugaagc agcugaagcg gagaagguac acuggcuggg gucgccuuuc ccgaaaauug     2220

auaaacggga uccgggauaa gcaaucuggc aaaaccauuc uggacuucuu aaagagcgau     2280

ggauuugcca accguaauuu caugcagcuc auccaugacg auucacugac auuuaaagag     2340

gacauucaga aggcacaggu gaguggccaa ggggacuccc uucacgaaca caucgcgaac     2400

cuggcuggaa gccccgccau aaagaaaggu auuuugcaga cugucaaggu ggucgaugag     2460

cucgugaaag uuaugggcag acauaagccg gaaaauaucg ugauugagau ggcaagggag     2520

aaccagacca cacagaaagg gcaaaagaac ucucgcgaac ggaugaaaag aaucgaggaa     2580

ggaauaaagg agcugggcuc acagauccua aaggagcacc cuguagaaaa uacccagcug     2640

cagaaugaga aacucuaccu guauuaccuu caaaacggua gggacaugua ugucgaucag     2700

gaauuggaca uuaaucgacu gagugauuac gacguggauc auaucguucc ccagagcuuc     2760

uuaaaggacg auuccauuga caacaaagug cucacucgcu cugauaagaa ucgggggaaa     2820

ucggacaacg ucccaagcga ggaaguggua aagaagauga aaaauuauug gagacaacug     2880

cuuaacgcca agcugaucac gcagaggaaa uuugacaauc uaacaaaggc ugagcguggc     2940

ggacucuccg agcuggauaa agccggcuuc auaaagcgcc aguuggugga aacccggcag     3000

auuacuaagc acguugcaca aauccuggac ucaagaauga acaccaaaua cgaugagaac     3060

gacaaguuaa ucagggaagu caaagugauu acacucaaga guaaacuggu gucugauuuu     3120

cgaaaggacu uccaguucua uaagguccgg gagaucaaua acuaccacca ugcucacgau     3180

gccuaccuua augcgguugu ggggaccgcc cugauuaaaa aguauccuaa auuggaaagc     3240

gaguuugugu acggagacua uaagguauac gauguccgca aaaugauagc uaaguccgag     3300

caggaaaucg guaaggcaac ugccaaauau uucuuuuacu caaacauuau gaauuucuuu     3360

aagacagaga ucacgcucgc uaacggcgaa auuagaaaaa ggccccugau cgagaccaau     3420

ggggaaacug gagagauagu gugggacaag ggccgggauu ucgccacagu ucgaaaagug     3480

cuaagcaugc cacaggucaa caucgugaag aaaaccgagg uucaaacagg uggguuuucu     3540

aaggaaucca uucugcccaa gagaaauagu gacaaacuua ucgcacgcaa gaaagauugg     3600

gacccuaaga aauauggcgg auucgacagc ccaacugugg cguacucugu acucgucgug     3660

gccaaggugg agaagggcaa auccaagaaa cugaagucag ucaaagaauu gcuggggauu     3720

accaucaugg agaggaguag cuuugagaag aacccgauag auuucuuaga agcuaaggga     3780

uacaaagagg uuaagaaaga ccucauuauc aagcugccca aauauucgcu uuuugaacug     3840

gagaauggcc guaagcggau guuggccucc gcuggugaac uccagaaggg gaacgagcug     3900

gcacuaccuu cuaaauacgu gaauuuccug uaucuugcca gccauuacga gaagcucaaa     3960

ggaucaccag aagauaacga gcagaagcag cuguucguag aacaacacaa acauuauuug     4020

gacgagauca uugaacagau aagugaguuu uccaagagag ugaucuuagc agaugccaau     4080

cuggacaaag uccucucugc uuacaacaag cacagggaua agcccauucg cgagcaggcg     4140

gaaaacauca uucaccuguu cacgcuuacc aaucugggcg ccccugcagc uuuuaaauau     4200

uucgacacaa cuaucgaucg aaagagauac accagcacaa aagagguguu ggacgccacu     4260

cuaauacauc aauccaucac cggucuguac gaaacgcgga uugaucucuc acagcugggc     4320

ggggacaaga ggccagccgc uaccaaaaag gcaggacagg ccaagaaaaa gaaauaagga     4380

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4440

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aagaauu                                                    4517


<210>  3635
<211>  5300
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3635
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaacuu caagauucug      240

ccaauagcca ucgaccucgg aguuaaaaau accggugugu uuagcgcguu cuaccagaag      300

ggcacauccc uugagaggcu ggauaacaag aaugggaaag uauaugaauu gucuaaggac      360

ucauacacuu uacugaugaa caaucggacg gcucgccgac aucaacguag aggaauugau      420

aggaaacagc uagugaagcg gcucuuuaaa cugaucugga ccgagcagcu uaaccuggaa      480

ugggacaagg auacacagca agccauuagu uucuuguuua aucgcagagg cuucagcuuu      540

aucacugacg guuauucccc ggaguaccuc aacauagucc ccgaacaggu gaaggcaauc      600

cugauggaua uuuucgacga uuauaauggg gaggacgauc uggacucuua ccucaaauua      660

gccaccgagc aggaaucgaa gaucucagag auuuauaaca aacugaugca gaagauccuu      720

gaauuuaaau ugaugaagcu gugcacagau auaaaagacg acaagguuag caccaagacu      780

cuaaaagaga uuacaaguua cgaguucgaa cuccuggcug auuaccuugc aaauuauucc      840

gagucucuga agacccaaaa auuuagcuac acggacaagc agggaaacuu gaaagaacuc      900

uccuauuacc accaugauaa guauaauauc caggaguucc ugaagaggca cgccacuauu      960

aacgaccgga ucuuagauac ccugcucaca gacgaucuug acaucuggaa uuuuaacuuc     1020

gaaaaauucg auuuugacaa gaacgaggag aaacugcaga aucaagaaga uaaggaccau     1080

auucaggcuc acuugcacca uuucguguuu gccgucaaca aaauaaaguc agagauggcg     1140

aguggcggcc gccaccgaag ccaguacuuc caagaaauca cuaaugugcu agacgagaac     1200

aaucaucagg aaggguaucu gaagaacuuu ugugagaauc uccacaacaa aaaguacucu     1260

aaucuguccg uuaaaaaccu uguaaaucug auuggaaacu ugucaaaucu cgagcugaag     1320

ccuuuaagaa aauacuucaa cgauaagauc cacgcaaagg ccgaccauug ggaugaacag     1380

aaauuuaccg agacauauug ccacuggaua cugggugaau ggaggguggg cgucaaggac     1440

caggauaaaa aggacggggc uaaauacagc uauaaggauc uauguaauga gcucaaacaa     1500

aaggugacca aggccggacu gguggacuuc cuuuuggaac uggauccaug ccggacgauu     1560

cccccuuacc ucgacaacaa caaucguaaa ccacccaagu gucagagucu gauccuuaac     1620

ccuaaauuuc uggauaauca guauccaaac uggcagcaau acuugcagga gcucaagaaa     1680

cugcagucua uccagaauua uuuagacucc uucgagacug aucugaaggu ccuaaagagc     1740

ucgaaagacc aaccguacuu uguugaauac aagucuucca accagcagau ugcaucaggc     1800

cagcgcgacu auaaagaucu cgacgcuaga auccugcaau ucauuuuuga uagggugaag     1860

gccagugacg agcuuuugcu gaaugaaaua uacuuccagg cgaaaaagcu caagcagaaa     1920

gccagcuccg agcuggagaa guuagaaucu agcaaaaagc uggaugaggu aaucgcaaac     1980

ucacaacuua gucagauuuu gaaaucccag cauacaaaug guaucuucga acaggggacc     2040

uuucuccacc uggugugcaa guauuacaag caacggcagc gagcucgcga cagcagacua     2100

uauaucaugc ccgaguacag guaugauaaa aagcugcaua aauacaacaa uacuggacgg     2160

uucgacgaug acaaccagcu ccugacauac uguaaucaca agccucgcca gaaaagauau     2220

caacuuuuga acgaucuggc cggcgucuua caggugucuc caaauuuucu caaggacaaa     2280

auuggcucag augacgaccu guucauaagu aaguggcuug uugaacacau caggggguuu     2340

aagaaagcau gcgaggauag ccugaagauu cagaaagaca accguggauu gcucaaccau     2400

aagaucaaua uugcccggaa caccaaaggu aagugugaga aggaaaucuu caaucugaua     2460

ugcaaaaucg agggcuccga agauaagaaa gggaacuaca agcacggacu agcauaugag     2520

cugggcgugc uccuguuugg ugaacccaau gaggccucua aaccugaguu cgaccgaaag     2580

auuaagaaau uuaacucgau cuacagcuuc gcucagauuc aacagaucgc guuugcagaa     2640

agaaagggga augccaacac gugugcuguc ugcuccgccg auaaugcaca uaggaugcag     2700

caaauaaaaa uuaccgagcc aguggaagac aacaaggaua aaaucauucu uucagccaag     2760

gcucagcgcu ugcccgccau cccgacacgg auaguagacg gcgcaguuaa gaaaauggcg     2820

acuauccugg cuaagaauau uguggaugac aacuggcaga auaucaaaca ggucuuaagu     2880

gccaagcacc aacuccacau uccuaucaua accgagucua acgcauucga auuugagccc     2940

gcccuggcug augugaaagg aaagagccuu aaggacagaa ggaaaaaggc ccuggagcgc     3000

auuuccccag aaaauaucuu caaagacaag aacaaccgaa ucaaagaguu cgcaaagggc     3060

auuucagcuu auagcggggc caaucuaaca gauggagacu uugauggugc gaaagaagaa     3120

cucgaccaua ucauuccucg gucucacaag aaguacggca cucugaacga ugaggccaau     3180

uugauaugcg ucacccgugg ggacaacaaa aauaagggaa acagaaucuu cugucugagg     3240

gauuuagcug acaauuauaa acucaagcag uuugaaacaa ccgaugaccu ggagauugag     3300

aaaaagaucg cagauacuau uugggacgcc aacaagaaag auuucaaguu uggcaauuac     3360

cgcuccuuca ucaaccuuac gccccaggag cagaaggcuu uucggcaugc ccuguucuug     3420

gcagacgaaa auccaauaaa acaagcggug aucagagcca uuaacaauag gaaccgaaca     3480

uuuguuaaug guacccagcg cuacuucgcu gaggugcucg ccaacaauau cuaucugcgg     3540

gcuaaaaagg agaaccuaaa cacugacaag auuaguuuug auuacuucgg gauccccacc     3600

auaggcaaug gaagaggcau ugcugaaauc aggcagcugu augagaaagu agacagcgau     3660

auccaggccu acgcaaaggg ggacaaaccu caagccucuu auucccaccu uauugaugcu     3720

augcucgcgu ucugcauagc cgcagacgaa caucguaacg auggaucaau cggucuggag     3780

auugacaaga auuacaguuu guauccacug gauaaaaaca caggcgaagu cuuuaccaag     3840

gacaucuuca gccagauuaa gaucacugau aaugaguuuu cggacaaaaa guuagugcgc     3900

aaaaaggcua uagagggguu caacacacac cggcagauga cgagagaugg aaucuacgcc     3960

gaaaauuacc ucccgauucu gauccacaaa gagcuuaacg aaguuaggaa gggcuauacc     4020

uggaaaaauu ccgaggaaau uaagaucuuu aaggguaaaa aguacgacau acagcaacug     4080

aacaauuugg uguauugucu caaauucguc gacaagccca uuucuaucga uauccagauu     4140

agcacucugg aggagcuacg aaacauccug acaaccaaua acauagccgc uacagcagaa     4200

uacuauuaca uuaaucuuaa aacucagaag cuccaugagu auuacaucga aaacuacaac     4260

accgcccugg gguauaagaa auacucaaag gagauggagu uuuugcggag uuuagcguau     4320

cgcuccgaaa gagugaaaau uaagucuauc gacgauguaa aacaagugcu ggacaaggau     4380

agcaauuuca uaauuggcaa gaucacgcuc ccuuuuaaaa aggaguggca gaggcuguac     4440

cgggaauggc agaacaccac aaucaaagac gauuaugagu uccuuaaguc cuuuuucaau     4500

guuaaaucaa uuacuaagcu gcacaagaaa guccgaaagg acuuuagcuu gccaaucagu     4560

accaacgaag gaaaauuccu agugaagaga aaaacauggg auaauaacuu cauuuaccag     4620

auacugaaug acucugauuc ccgcgcugac ggcacuaagc ccuuuauccc ugccuucgac     4680

auuagcaaaa acgagaucgu ggaggcaauc auugauucgu uuaccucaaa gaauauauuc     4740

uggcucccaa agaacaucga acugcaaaaa gucgacaaua agaacauuuu ugccaucgau     4800

acgucuaaau gguucgaggu ugaaacaccc agugaccuua gggauauugg gaucgcuacc     4860

auacaguaua agaucgacaa uaacucccgu ccgaaagugc gggugaaguu agauuacgua     4920

auugacgaug acagcaagau caauuacuuu augaaccauu cucugcucaa aagcagauau     4980

ccugauaagg ucuuggagau ucugaaacag uccacuauca uagaguucga aucaagugga     5040

uuuaacaaga caauuaaaga gaugcucggc augaagcugg cagguaucua caaugaaacc     5100

agcaacaaua agaggccagc cgcgacuaaa aaggcugggc aggccaaaaa gaaaaaguaa     5160

ggacuagucc cuguucccag agcccacuuu uuuuucuuuu uuugaaauaa aauagccugu     5220

cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5280

aaaaaaaaaa aaaaagaauu                                                 5300


<210>  3636
<211>  3572
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3636
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagag gaacuacauu      240

cugggacucg acauagguau cacaucaguu ggcuauggga uuaucgauua cgagacacgg      300

gacgugauug augccggagu acgccuuuuc aaagaagcga auguggagaa caaugaaggc      360

cgacguucca agagaggugc uaggcggcug aagcgcagaa ggcggcaucg cauccagcga      420

gucaaaaagu uguuauuuga cuauaaccug cuaacugauc acucugagcu cucagggaua      480

aauccauacg aagccagagu gaaaggacug agucaaaaac uuagcgagga ggaguucucc      540

gcagcccugu ugcaucucgc uaaaaggcgg ggcguucaca acgugaauga agucgaagag      600

gacacgggca acgagcuguc uaccaaggaa cagaucucgc guaauucaaa ggcacuggag      660

gaaaaauaug uggccgagcu ccaguuagaa cgccugaaga aagaugggga gguaagagga      720

agcauuaaca gguuuaagac aagugacuac guuaaagagg cuaagcagcu uuugaaagug      780

caaaaggccu aucaucagcu ggaucagucc uucaucgaca cuuacauuga ucuacucgaa      840

acccggcgaa cauacuauga ggguccgggc gaagggucuc ccuuuggaug gaaggacauc      900

aaagaguggu acgaaaugcu gaugggccac ugcaccuauu ucccugagga gcuucgcagc      960

gucaaguacg cguauaaugc agaucuguac aacgccuuga augaccucaa caaucuggug     1020

auaacuagag acgaaaacga gaaauuagaa uauuacgaga aguuucagau uaucgaaaac     1080

guguucaaac aaaagaagaa accaacacug aagcagauug cuaaagagau ccucgucaau     1140

gaggaagaua ucaaggguua caggguuacc uccacgggga aacccgaguu uacuaaccuu     1200

aagguguauc acgacauuaa ggauauaacc gcccggaaag aaaucauuga gaaugcagag     1260

cuguuggacc agaucgcuaa gauacuaaca auuuaccagu caagugaaga uauccaagag     1320

gaacugacua accucaauag cgagcugacc caggaagaga ucgagcagau uucuaaccuu     1380

aaaggauaua caggcaccca uaaucugucc uugaaggcca ucaaccucau ucuggacgaa     1440

uuauggcaca cgaaugauaa ccaaauagcg aucuucaauc gccugaaacu uguaccuaag     1500

aagguggacc ucucacagca gaaagagauu ccaacuacac uggucgauga cuuuauccuu     1560

agccccgugg uuaagagaag uuucauccag ucuauuaaag ugauaaacgc caucauuaag     1620

aaauacggcu ugccuaauga uaucauuauc gaacuggcaa gggagaagaa cuccaaagac     1680

gcucaaaaga ugauaaauga aaugcagaag cguaaccggc agaccaacga gcgaaucgag     1740

gaaauuauca gaacuacagg gaaagagaau gccaaguauc ucauugaaaa gaucaagcug     1800

caugacaugc aggagggaaa augucuuuac agccuggaag cuauaccauu ggaggaucuc     1860

cugaacaauc cguuuaacua ugaggucgac cacauuaucc ccaggucggu gucuuucgau     1920

aauuccuuca acaauaaggu auuaguuaag caagaagaga acucaaagaa ggguaaucgc     1980

accccuuuuc aguaccugag uagcuccgac ucuaaaauua gcuacgaaac guucaagaaa     2040

cacauccuaa accucgccaa gggcaagggg cggauaucaa aaaccaagaa agaguaucug     2100

cuugaggaaa gagauaucaa uagguuuagu gugcagaagg acuucauuaa ccgcaauuug     2160

gucgauacac gauacgcaac ucggggacug augaaccucc ugcguuccua uuuuagagug     2220

aauaacuuag acgucaaagu gaaaagcauc aacggcgguu ucaccucuuu ucugaggcgc     2280

aaguggaaau ucaagaaaga gcggaauaag ggguacaaac aucacgccga agaugcucuu     2340

auuaucgcga acgcagacuu uauauucaag gaguggaaaa aguuggauaa ggccaaaaag     2400

guuauggaaa aucagauguu ugaggagaaa caagcugaau caaugccaga gauugaaaca     2460

gagcaggaau auaaggagau cuucaucacu ccccaucaga uuaaacacau caaggacuuu     2520

aaggauuaca aauauaguca uagaguggac aagaaaccua acagggagcu cauuaaugac     2580

acccuguaca gcacacgaaa ggaugacaaa ggcaacaccc uaauaguaaa uaaccugaau     2640

ggacucuacg auaaggacaa cgauaagcug aaaaagcuaa ucaauaaauc cccagaaaag     2700

uugcugaugu aucaccacga cccccagacu uaccaaaaau uaaagcucau uauggagcag     2760

uauggcgaug aaaagaaccc gcuguacaaa uauuacgagg aaacggggaa uuaucuuaca     2820

aaguacucua aaaaggacaa cggaccuguc aucaaaaaga uuaaguacua ugguaauaaa     2880

cugaacgccc auuuggauau caccgacgau uaccccaacu cgcgcaauaa ggugguuaaa     2940

cucagccuga agccauaucg guucgacgug uaccuagaua acggcgucua uaaauucgug     3000

acuguaaaga aucuggacgu gaucaaaaag gagaacuacu augagguuaa uuccaagugc     3060

uacgaagagg ccaaaaagcu caaaaagaua ucaaaccagg cagaauuuau ugcuaguuuc     3120

uacaauaacg accugaucaa aauuaauggg gagcuuuaua gagucaucgg agugaacaau     3180

gauuugcuga acaggauaga ggugaauaug auugacauca ccuaccguga auauuuagag     3240

aacaugaaug auaagcgccc uccccggauc auuaagacaa uagccucuaa aacccaaagc     3300

aucaagaaau acuccacuga cauucucggc aaccuguaug aagucaagag caaaaagcac     3360

ccacagauca uuaagaaagg uaagagaccc gcagcgacca aaaaggcugg gcaggccaaa     3420

aagaagaaau aaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau     3480

aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3572


<210>  3637
<211>  4640
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3637
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcacuguu caacaagugc      240

auuauaauca gcauuaaucu cgacuuuucc aacaaagaga aguguaugac caagccauac      300

ucuaucggac uugauauugg uacaaauuca guuggcuggg ccgugaucac ugacaacuau      360

aaaguaccga guaagaaaau gaaggugcug gggaauacga gcaaaaagua cauaaagaaa      420

aacuuguuag gaguccugcu auucgauucc ggcaucaccg cggaagguag gcggcucaag      480

cgcacagcuc gacguagaua uacuaggcgg cgcaauagga uucuguaccu ucaggagauc      540

uuuucuaccg aaauggccac acuggacgau gcauucuuuc aaagauugga cgauucguuc      600

cucgugcccg acgauaaacg ggacucaaag uauccuauuu uugggaaccu gguugaggaa      660

aaaguguacc augaugaguu cccaaccauc uaucaucugc gcaaguaccu cgccgacagc      720

acuaaaaagg cugauuuacg acuggucuac cuugcauugg cccacaugau aaaguauaga      780

ggacacuuuc ugauugaggg cgaguucaau aguaaaaaca augacaucca gaagaacuuu      840

caggacuucc uagauacaua caaugcuauu uuugaguccg accucucucu ggaaaacagc      900

aaacagcuug aggagaucgu gaaggauaaa aucuccaagc uggaaaagaa agacaggauu      960

uugaaacucu uccccggcga gaaaaauuca gggauauuca gugaauuucu gaaguuaauc     1020

guaggaaacc aagccgauuu ccggaaaugc uuuaaccugg acgagaaggc gagccuccau     1080

uucucuaagg aauccuauga ugaggaccuu gagacccugu uggguuacau uggcgaugac     1140

uauucagaug uuuuucuaaa agcaaagaaa cuguacgacg ccauccuccu gagcggguuc     1200

cuuacgguga cugacaauga aaccgaggcu ccucugaguu cugccaugau aaagcguuau     1260

aacgaacaca aagaggauuu ggcacuccug aaggaauaca uucgcaauau cuccuuaaag     1320

acauacaacg aggucuuuaa agacgauacu aagaauggau augcuggcua caucgacggu     1380

aaaaccaacc aggaggauuu cuauguguac cugaagaauc uacucgccga auuugagggg     1440

gcggacuauu uccuggaaaa gauugauaga gaggacuuuc uuaggaagca gcggacauuc     1500

gauaacggaa gcaucccaua ccagauucac uugcaagaaa ugcgagccau acuggacaaa     1560

caggcaaagu uuuaucccuu ccucgcuaag aauaaagagc gcaucgagaa gauucugacc     1620

uucagaaucc cuuacuacgu gggcccacuu gccaggggca acucggauuu ugcuuggucu     1680

auccggaaac gcaaugaaaa gauuacgccg uggaacuucg aggacgucau agauaaagaa     1740

uccucagccg aggcauuuau caauagaaug acaaguuucg accuguauuu gcccgaggaa     1800

aagguucucc cuaagcauag ccuguuauac gagacuuuua acguguauaa ugaacugacc     1860

aaaguaaggu ucauugccga guccaugcgu gacuaccagu uucuagauuc uaagcagaaa     1920

aaggacaucg ugcggcucua uuucaaagau aagcgaaagg ucacugacaa agauauuauc     1980

gaauaccugc acgcuauaua uggguacgac ggaaucgagc uuaaggguau ugagaaacaa     2040

uuuaacagcu cauugaguac auaccaugau cugcucaaca ucauuaauga caaggaguuc     2100

cuggaugacu ccagcaacga agcgaucaua gaagagauua uccacaccuu aacgauuuuu     2160

gaagauagag agaugaucaa acagaggcug ucuaaguucg agaauauauu ugacaaguca     2220

gugcuuaaaa aguugagucg ccggcacuau accggcuggg ggaaacucag cgcaaagcug     2280

aucaacggaa uuagagauga aaaauccggc aauacaaucc uagacuaccu gauugacgau     2340

gguaucucua acaggaauuu caugcagcuc auacaugacg augcccuguc guucaagaaa     2400

aagauucaaa aggcucagau caucggggac gaggauaaag gcaacauuaa ggaaguugug     2460

aaaagccuuc caggaucccc cgccaucaag aaaggcauuu ugcagucaau aaagaucguc     2520

gacgagcugg ugaagguaau gggagggcgc aaaccugaaa guauuguugu ggagauggca     2580

cgagagaauc aguauacuaa ccaagguaag ucuaauagcc agcagcgguu aaaacgucuc     2640

gaaaaguccc ugaaagagcu uggcucaaag auccugaagg aaaacauucc agccaaauug     2700

agcaagaucg auaauaacgc ucuccagaau gacagacugu accuauauua ccugcaaaac     2760

gggaaagaua uguauaccgg agacgaucuc gacauagaca ggcugucuaa cuacgauauc     2820

gaccacauua ucccccaggc cuuucuuaag gauaauucca uugacaacaa agucuuggug     2880

aguagcgcau cuaaucgcgg caaguccgau gacuucccgu cacuggaggu cgugaagaaa     2940

cggaagacau uuugguacca guuacucaaa aguaagcuga ucagccagag aaaauucgau     3000

aaccuuacua aggcggagag gggugggcug cuaccugaag acaaggcugg cuuuauacaa     3060

cgacagcucg uugagacccg ccagauuaca aaacaugugg cccggcuguu ggaugaaaag     3120

uucaauaaca aaaaggacga gaauaacaga gcaguaagga ccgucaaaau caucacucug     3180

aagucgacgu uaguguccca guuucguaaa gauuucgaac ucuauaaggu ucgcgagauu     3240

aaugacuuuc accaugccca cgaugcuuac cugaacgccg ugauagcauc ugcccuucug     3300

aagaaauauc ccaaguugga gccagaguuc gucuacggag acuauccuaa auacaauagc     3360

uuucgggaaa gaaagucagc uacagaaaaa guguauuucu acaguaacau caugaauauu     3420

uucaagaagu ccaucucucu cgcggacggc aggguaauug agcgaccccu gaucgaagug     3480

aacgaggaga ccggggaaag cguuuggaau aaagaguccg aucuagccac uguccggcgc     3540

gugcugucau acccacaagu gaacgucguu aagaaagugg aagaacagaa ccacggacuu     3600

gacagaggua agcccaaagg ccucuuuaau gcuaaccuga gcaguaagcc uaagccaaau     3660

ucuaacgaaa auuugguggg ggcaaaagag uaucuggauc cgaagaaaua cggaggcuau     3720

gccgguauau ccaacagcuu cgcuguauua gucaagggga ccaucgagaa aggcgccaag     3780

aagaaaauua caaaugugcu cgaauuucag ggaaucucga uucuggacag gaucaacuac     3840

cggaaggaua aacuuaauuu ccuguuggag aagggcuaua aagacauaga acucauuauc     3900

gagcugccca aguacucacu auuugagcug ucugauggga gucgaagaau gcuugcaucc     3960

auccucagca ccaacaauaa acgcggagaa auucauaagg gcaaccaaau cuuccugucu     4020

cagaaguuug uuaaauuguu auaucacgcg aagaggauaa gcaauacuau uaacgagaau     4080

caucguaaau acguggaaaa ccacaagaaa gaguucgagg aacuguuuua cuauauccuc     4140

gaguucaaug aaaacuacgu cggugccaag aagaacggga aacugcuuaa uuccgcuuuu     4200

cagucauggc agaaccacag uauugacgag cuguguagcu cuuucaucgg accuacaggc     4260

uccgaacgga aggguuuguu ugagcuaacg ucaagaggca gugccgcaga uuucgaguuc     4320

cuggggguga aaauaccaag guaucgcgac uacaccccca gcucccuccu gaaggaugcu     4380

acucuuauuc aucaaucugu uacaggauua uaugaaaccc gaaucgaccu ggccaaacuc     4440

ggcgagggga agagaccugc agccacaaag aaagcuggac aggcgaagaa aaagaaauaa     4500

ggacuagucc cuguucccag agcccacuuu uuuuucuuuu uuugaaauaa aauagccugu     4560

cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaagaauu                                                 4640


<210>  3638
<211>  3716
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3638
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcauggua cgccagccug      240

auguccgcgc aucaccucag gguuggaauu gacgugggua cccauucugu aggccuugcu      300

acacugcggg uggaugacca cgggacucca auagaguugu uaucagcccu gagucauauc      360

cacgauagcg gagucggcaa ggaagguaaa aaggaccacg auacgcgcaa gaaacuaucc      420

gggauugcac gacgugccag aaggcuccug caucaccggc gcacccagcu ucaacagcug      480

gacgaggugu ugagagaucu cggauucccg auccccacac cuggcgaauu ucuggaccug      540

aacgagcaga cugauccaua uaggguuugg cgggugcgcg cucgacucgu cgaagagaag      600

uuacccgagg aacugagagg cccugcaauu ucuauggccg ugaggcauau cgcucggcac      660

cguggguggc gcaauccaua cucgaaagua gagucacuuu ugagccccgc cgaagagagu      720

ccuuucauga aggcgcugag agagaggaua cuagcaacca caggagaagu ucucgacgau      780

gguaucaccc caggccaggc cauggcucaa guggcccuga cucacaacau uuccaugcgg      840

gggccggagg gaauccuugg caaacugcau cagucugaca augcaaacga aauucgaaag      900

aucugcgcuc gccagggugu cagccccgau guguguaagc aguugcucag agccguguuu      960

aaagcggacu ccccuagggg gucagccguc agucggguug caccagaccc ccugccugga     1020

caaggcagcu uccgcagagc uccaaagugc gaucccgagu uucagagguu ccguauaauu     1080

ucuaucgugg ccaauuuacg gauuuccgaa acaaaaggcg agaaccgacc gcugaccgcu     1140

gacgagagaa ggcacguagu gacguuucuc acugaagauu cacaggccga ccuuaccugg     1200

gucgaugugg cagagaagcu ggggguucau cgccgggacu ugagaggaac agccgugcac     1260

acugaugacg gugaaaggag cgcugcgcgc ccucccaucg augcaaccga ccgaaucaug     1320

cggcagacaa aaauuaguuc ucuaaagacc uggugggagg aagccgauuc cgagcaacgu     1380

ggcgcuauga uaagauaucu guacgaggac ccaacggaca gcgaaugugc cgagaucauu     1440

gcagaacucc cugaggaaga ucaggccaaa cuggacucgc uucaucugcc cgcugggagg     1500

gccgcauauu cucgcgaguc cuugacugcg cucucagauc acaugcuggc uacaaccgac     1560

gauuuacacg aggcccggaa gagacuguuc ggagucgacg auaguugggc accacccgcc     1620

gaagcuauca augccccugu gggcaaccca agcguagaca ggacucuaaa gauaguuggu     1680

cgauaccucu ccgcagugga gucuaugugg gggacaccgg aagucauuca uguggagcac     1740

guccgcgaug gcuuuaccag cgagcggaug gcugacgaaa gagauaaagc caauaggcgu     1800

cgcuauaacg acaaucagga ggcgaugaag aaaauccaac gggauuacgg aaaggaaggc     1860

uacaucucaa gaggggacau ugugaggcug gacgcccuug aguugcaggg augcgcuugu     1920

cuguauugcg guacgaccau cggcuaccau acaugucagc ucgaucacau uguuccccag     1980

gcagggccug gaaguaacaa ucgacggggc aaccuggugg ccguaugcga acgcuguaau     2040

agauccaaaa gcaacacucc auucgcuguc ugggcccaaa agugcgguau accccacgug     2100

gggguuaagg aggcaaucgg cagggugcgg ggauggcgaa aacagacccc uaacacaucu     2160

ucagaggacc uuacuagacu gaagaaagaa gucauugcgc gcuugaggcg uacccaggag     2220

gauccagaaa ucgacgagcg gaguauggaa agcguggccu ggauggcuaa ugagcuccau     2280

cacagaaucg ccgcagcuua ucccgagaca accguaaugg uguacagggg cuccauuacu     2340

gccgcagccc gcaaggcugc ggggauagau ucucgaauca accugauugg agaaaaaggu     2400

agaaaggacc ggaucgauag gcgccaucac gccguugacg caucggucgu ggcuuuaaug     2460

gaggccagcg uggccaagac gcuggcugaa cguuccucac uaagaggcga gcagcggcuc     2520

acagggaaag aacaaaccug gaagcaguau acuggaagua ccgucggcgc aagggagcau     2580

uuugagaugu ggcgcgguca caugcugcac cuuacagaau uguucaauga gcgacuggcc     2640

gaagauaaag uuuacgugac ccagaacauu agacuccggc ugucugacgg gaaugcgcau     2700

acugugaacc cgagcaaguu aguaucccac aggcugggcg auggacuuac aguccagcaa     2760

aucgaccgcg cuuguacgcc ugccuugugg ugcgcacuca ccagagagaa agauuuugac     2820

gagaagaaug gccugccagc cagggaagac cgggcuauac gugugcaugg gcacgagauc     2880

aagucaagcg auuauauuca gguuuucucu aaacgcaaga aaacugacuc cgaucgagac     2940

gaaacacccu ucggagcaau cgccgugaga ggcgguuuug ucgagauugg gccuaguauc     3000

caucacgcga ggauauaccg gguggaagga aagaaacccg uuuacgcuau gcuacgcgug     3060

uucacccacg aucugcucag ccagagacau ggcgaccugu uuucugccgu aauuccaccu     3120

caguccaucu caaugaggug cgcagagccc aaacuucgga aagccauuac aacugguaac     3180

gcuaccuauu ugggcugggu cguggugggg gaugagcugg aaaucaaugu cgacaguuuc     3240

acgaaguacg ccauaggacg auuuuuagag gauuucccaa acaccacaag auggcgcauc     3300

uguggcuaug acacuaauag caagcucacc cugaaaccga uuguucuugc agcugaaggg     3360

cuggagaacc cuucguccgc cgugaaugaa aucguagagu ugaagggaug gaggguggcg     3420

auuaacgucc ucacaaaagu gcaccccacu guugugcggc gugaugcccu gggcagacca     3480

agguacucua gccgcucaaa ucuacccacc aguuggacga ucgagaagcg gccugcagcu     3540

acaaagaagg ccggucaagc uaagaaaaag aaauaaggac uagucccugu ucccagagcc     3600

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         3716


<210>  3639
<211>  4577
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3639
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaccaa gccauacagc      240

auuggacugg acauagguac aaacuccguu ggcugggccg ugacuacgga uaauuauaaa      300

guaccgucua agaagaugaa agugcucggg aacaccucaa agaaauacau caagaaaaau      360

cuucugggag ucuuguuauu cgacaguggc auuacagcgg aggguaggcg gcugaagcgc      420

acugcucgac guagauauac caggcggcgc aacagaaucc uauaccucca ggaaauuuuu      480

agcacagaga uggccacccu ggaugacgca uucuuucaaa ggcuugauga cuccuuccug      540

gugcccgaug acaagcggga uucuaaauau ccuaucuuug ggaauuuggu ugaagagaag      600

gccuaccaug acgaguuccc aacuauauau caccuccgca aauaccuggc ugauucgaca      660

aagaaagcag accugcgacu cguguacuua gcccuggcuc auaugaucaa guauagagga      720

cacuuucuua uugagggcga guucaacuca aagaauaacg auauccagaa gaauuuucag      780

gacuucuugg acaccuacaa cgccauuuuu gaaagcgauc ugagucuaga aaauuccaag      840

cagcucgaag agaucgucaa agacaagaua ucuaaacugg agaagaaaga uaggauucuu      900

aagcuguucc ccggcgaaaa gaacagcggg aucuuuuccg aguucuugaa acucauugug      960

ggaaaucaag cggacuuccg gaagugcuuu aaccuggaug aaaaagcauc auuacauuuc     1020

aguaaggaga gcuaugacga agaucuggag acgcuccuug guuacaucgg cgacgauuau     1080

ucugacguau uucugaaagc caagaaguug uacgaugcua uccuacuguc cggguuccuc     1140

acuguuaccg acaaugagac agaagccccu cugucaagcg caaugauuaa acguuauaac     1200

gagcacaagg aagaccuugc ucuguugaaa gaguacauac gcaacaucag ucucaagacu     1260

uacaaugaag uguuuaaaga ugacaccaag aacggauaug ccggcuacau ugaugguaag     1320

acaaaucagg aggacuucua ugucuaccug aaaaaguuac uggcggaguu ugaaggggcc     1380

gauuauuucc uagagaaaau cgacagagaa gauuuucuca ggaagcagcg gaccuucgac     1440

aacggaucua uaccauacca gauucaccuu caagagaugc gagcaauccu ugauaaacag     1500

gcuaaguuuu aucccuucuu ggccaaaaau aaggaacgca ucgagaagau ucugacguuu     1560

agaaucccuu acuacguggg cccacucgcu aggggcaacu ccgacuucgc cuggagcauu     1620

cggaaacgca augagaagau aacuccgugg aacuucgaag augugaucga caaagagucg     1680

ucugcagaag ccuuuauuaa uagaaugaca uccuucgauc uguaucuucc cgaggagaag     1740

guccugccua aacauucauu gcucuacgaa accuuuaacg uuuauaauga gcugacuaag     1800

gugagguuca ucgcugaaag uaugcgugac uaccaguuuu uagacagcaa gcagaaaaag     1860

gauaucguac ggcuguauuu caaagacaag cgaaaaguga cagauaagga cauuauagag     1920

uaccuacacg cgaucuaugg guacgaugga auugaacuca aggguaucga gaaacaauuu     1980

aacuccucuc ugagcaccua ccaugaccuu uugaauauua ucaacgauaa ggaguuccug     2040

gacgauucaa guaaugaagc aauaaucgag gaaauuaucc acacgcucac cauuuuugag     2100

gacagagaaa ugaucaaaca gaggcugucc aaguucgaga acauauuuga uaaaagcguc     2160

uuaaagaagc ugucucgccg gcacuauaca ggcuggggga aacuuucagc caaguugauu     2220

aacggaauca gagacgagaa aaguggcaau acuauucucg auuaccugau cgacgacggu     2280

auaagcaaca ggaauuucau gcagcuaauc caugaugacg cucuguccuu uaagaaaaag     2340

auucaaaaag cccagaucau uggggaugaa gacaagggca acaucaagga ggugguuaaa     2400

ucucucccag gaucgcccgc aauaaagaaa ggcauucugc agagcaucaa gaucguggau     2460

gaacuuguca aagugauggg agggcgcaag ccugagucca uuguaguuga aauggcccga     2520

gagaaucagu auaccaacca agguaaguca aauagucagc agcgguugaa acgucuggag     2580

aagucuuuaa aagaacucgg cagcaagauc cugaaagaga acauuccagc uaagcugucc     2640

aagauagaca auaacgcccu gcagaaugau agauuguacc ucuauuaccu gcaaaacggg     2700

aaagacaugu auacaggaga ugaccuagau aucgacaggc ugucaaauua cgacauugau     2760

cacaucauuc cccaggcauu ccucaaggac aacagcaucg auaauaaagu gcuggucucu     2820

uccgcgagua accgcggcaa gagcgacgau gugccgucuc uugaagucgu gaaaaagcgg     2880

aagacuuucu gguaccaguu gcugaaaucc aaguuaauau cacagagaaa auuugacaac     2940

cucaccaagg cugagagggg ugggcugagu ccugaggaua aagccggcuu cauccaacga     3000

cagcuuguug aaacacgcca gauuaccaag cauguggcac ggcugcuaga cgagaaguuu     3060

aauaacaaaa aggaugaaaa uaacagagcc guaaggacug ucaaaaucau uacgcucaag     3120

agcacacugg ugucgcaguu ccguaaagac uuugaguugu auaagguucg cgaaaucaau     3180

gauuuccacc augcucacga cgccuaccug aacgcagugg ucgcuuccgc cuuacucaaa     3240

aaguauccca agcuggagcc agaguuugug uacggagauu auccuaaaua caauucuuuc     3300

cgggaaagaa agagcgcgac cgagaaagua uauuuuuacu caaacauaau gaauauuuuc     3360

aagaaaagua ucucccuugc cgacggcagg gugaucgaac gaccccugau ugagguuaac     3420

gaagagacug gggagucugu cuggaauaag gaaagcgacu uggcuaccgu gcggcgcgug     3480

cucuccuacc cacaagucaa cguugugaag aaaguggagg aacagaauca cggacuggau     3540

agagguaagc ccaaaggccu auuuaacgca aaucugucaa gcaagccuaa accaaacagu     3600

aacgagaauc uuguaggggc caaggaauau cucgacccga agaaauacgg aggcuaugcu     3660

gguauaucua acuccuucac aguccuggug aaggggacca ucgagaaagg cgccaagaaa     3720

aagauuacua auguuuugga guuccaggga aucagcauuc uggauaggau caacuaccgg     3780

aaggacaaau uaaauuuucu ccuggaaaag ggcuauaaag auauagagcu uaucauugaa     3840

cugcccaagu acucguuguu cgagcucuca gacgggucuc gaagaaugcu ggcaaguauc     3900

cuauccacaa acaauaaacg cggagagauu cauaagggca accaaaucuu ucugagccag     3960

aaauucguga aacuucucua ucacgcgaag aggauaucua auacgauuaa cgaaaaucau     4020

cguaaauacg ucgagaacca caagaaagaa uuugaggaac uguucuacua uaucuuggag     4080

uuuaaugaga acuacguggg ugccaagaaa aaugggaagu uacugaacag cgcuuuccag     4140

uccuggcaga aucacucaau cgaugaacuc uguaguagcu uuauuggacc uaccggcucu     4200

gagcggaagg gucuguucga acuuacuucc agaggcucag ccgcagacuu ugaguuccug     4260

gggguuaaaa ucccaaggua ucgcgauuac acacccagua gcuugcuaaa ggacgcuacc     4320

cugauacauc aauccgugac aggacucuau gaaacucgaa uugaucuggc caaacuuggc     4380

gaggggaaga gaccugcagc caccaaaaag gcuggacagg cgaagaaaaa gaaauaagga     4440

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4500

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aagaauu                                                    4577


<210>  3640
<211>  3659
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3640
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagccgc guucaagcca      240

aacagcauua auuacauacu gggacucgac aucgguauug cuuccguugg cugggccaug      300

guggagaucg augaagagga aaacccgauu aggcuuaucg accugggggu acggguguuu      360

gagcgcgcag aaguccccaa aaccggagau ucuuuggcca uggcucgacg uuuagcaaga      420

ucagugaggc ggcugacacg cagaagggcc caucggcuac uccgcacucg aagacugcuu      480

aagagggagg gcguucugca ggcugccaau uucgacgaga acgguuugau aaagagucuc      540

ccuaauacgc cauggcaacu gcgggcggca gcccuggauc guaaacucac ccccuuagaa      600

uggagcgcug ugcugcuuca cuugaucaag caucgcgggu aucuguccca gaggaaaaac      660

gagggagaaa cagccgacaa ggagcuaggc gcacuccuga aaggcgucgc ugggaaugcc      720

cacgcgcuuc agacuggaga uuuuaggacc ccugccgagc uggcauugaa caaguucgaa      780

aaggagucug gucauauucg gaaucagcga ucggacuacu cacacacauu uagccgcaaa      840

gaucuccaag cugaacugau cuuacuguuc gagaagcaga aagaauuugg caacccacac      900

gugagugggg gacucaagga gggcauugag acccuucuga ugacucagag acccgccuug      960

uccggugacg cuguacagaa aaugcuaggg cauugcacau ucgaaccugc cgagccaaag     1020

gcagccaaaa auaccuauac ggcugaaagg uuuaucuggc ugacuaagcu caacaaucug     1080

cggauacuug agcaaggauc ugaacgcccg cugaccgaua cagagagagc gacuuugaug     1140

gacgagcccu acagaaagag caaacucacc uaugcacagg cccguaagcu guuaggccug     1200

gaagauacag cuuucuuuaa aggccuacgg uacgggaagg acaacgccga ggcauccacc     1260

cucauggaaa ugaaagccua ucacgcuauu ucacgagccc uggagaagga aggacuuaag     1320

gacaaaaaga guccuuugaa ucugagccca gagcuccagg augagaucgg uacggcauuc     1380

ucucuguuua aaacugacga agauauuaca ggcagacuua aggacaggau ccagcccgag     1440

auccuggaag cguugcucaa acauauuucc uucgauaagu ucguucaaau aucacugaag     1500

gcuuuacgcc ggaucgugcc ucugauggag caggggaaaa gauacgacga ggccugugca     1560

gaaauuuacg gagaucacua uggcaagaaa aacaccgagg aaaagaucua ccuaccaccc     1620

auaccggccg acgagauuag gaacccuguc gugcuccgcg cucugagcca ggcccgaaaa     1680

gugaucaaug gugucguucg gcguuauggg agucccgcaa gaauccacau ugaaacugcu     1740

agggaggugg gcaagucuuu uaaggaucgc aaagagaucg aaaagcggca agaggaaaac     1800

agaaaagaca gggagaaggc cgcggccaaa uuccgagaau acuuuccaaa uuucguagga     1860

gagccuaagu ccaaagauau ucuuaaguug cgccuguaug agcagcagca uggcaagugc     1920

cucuacagcg ggaaagaaau aaaccuggga cgguuaaaug agaaggguua uguggaaauc     1980

gaccacgcuc ugcccuuuuc gagaacaugg gacgauucuu ucaacaauaa aguccuugug     2040

uugggcuccg agaaccagaa uaaggggaac caaaccccau acgaauacuu uaauggaaaa     2100

gacaacucaa gggaguggca ggaguucaag gcacguguug aaacgagucg cuuuccccgg     2160

agcaagaaac agaggauucu ccugcagaag uucgaugagg acggcuuuaa agaaagaaau     2220

cuaaacgaua cccgauaugu gaaucgguuc cugugucaau uugucgccga ccgcaugaga     2280

cucacaggua aggggaaaaa gaggguguuc gcuuccaacg gccagaucac uaaccugcuu     2340

cggggauucu ggggcuugcg aaagguaaga gccgagaaug aucgccauca cgcacuggac     2400

gcgguugugg ucgccugcuc uaccguggcu augcagcaga aaaucacaag guuuguccgu     2460

uacaaggaga ugaacgccuu cgaugggaaa acuauugaca aggaaaccgg agagguguua     2520

caucagaaaa cacacuuucc ucagccaugg gaguucuuug cacaggaagu uaugauacgg     2580

guguucggua agccggaugg caagcccgaa uuugaggagg cugacacccu cgaaaaacug     2640

agaacucuuc uggccgagaa guugagcuca aggccugaag caguacacga guaugucacg     2700

ccacucuucg ugagucgcgc ccccaaucga aaaauguccg ggcagggaca uauggaaaca     2760

guuaagagcg cuaaaagacu ggaugagggc gugucugucc uacgggugcc ucugacccaa     2820

cucaagcuga aagaccuuga gaagauggua aacagggaac gcgagccaaa guuguacgaa     2880

gcgcugaaag cccguuuaga ggcacacaag gacgaucccg cuaaagccuu ugccgaaccg     2940

uucuauaagu acgacaaagc ugguaauaga acucaacagg ugaaggcagu ucgggucgag     3000

caagugcaga agaccggggu gugggucagg aaccauaaug gcaucgccga uaacgcgaca     3060

augguucgcg uggacguguu ugagaaagga gauaaguauu accucguacc uauuuacuca     3120

uggcaggucg cuaaaggcau ccugccagac cgagccgugg uucaggggaa ggaugaagag     3180

gacuggcaac uuauugauga caguuucaau uuuaaauuca gccugcaccc caacgaucua     3240

guggaaguca ucaccaagaa ggcaagaaug uucggauauu uugccuccug ucaccggggc     3300

acugguaaua uaaacaucag gauucaugac cucgaucaca aaaucgggaa gaauggcauu     3360

cuggagggaa ucggugugaa aacagcuuug ucuuuccaga aguaccagau agacgagcug     3420

ggcaaagaaa uucgcccuug cagauuaaag aagaggcccc caguucggaa acguccugca     3480

gccacgaaga aagcggggca ggcuaagaaa aagaaguaag gacuaguccc uguucccaga     3540

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      3659


<210>  3641
<211>  4415
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3641
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagaa accauacacc      240

auuggacugg acauagguac aaacagcguu ggcugggccg ugcucacuga ucaguaugac      300

cuuguaaaga ggaagaugaa aaucgcgggg gauuccgaga agaaacaaau uaagaaaaau      360

uucuggggag ugcggcuguu ugacgaaggc cagacggcug ccgaucgccg aauggcacgu      420

accgccagaa ggcggaucga gcgcagaagg aaccggauuu cuuacuugca ggguaucuuc      480

gcugaagaga ugucaaagac agacgcaaac uuuuucugcc gcuuaaguga uagcuuuuau      540

gucgacaaug aaaagcgaaa uuccagacau ccguucuuug ccacuauaga ggaggaagug      600

gaguaccaca aaaacuaucc caccaucuac caucugaggg aggagcuagu uaauucuucg      660

gaaaaggcug aucuccggcu gguguaucuu gcccuggcgc acauuaucaa auaccguggg      720

aacuucuuga uugaaggagc acucgacaca cagaauaccu cagucgaugg caucuacaag      780

caauuuauac agacuuauaa ccagguguuc gccagcggca uugaggacgg gagucugaaa      840

aagcuggaag auaauaaaga cguagcuaag auccucguug agaaggugac acgcaaagaa      900

aaguuagaga ggauucugaa acuuuacccu ggagagaagu ccgccgguau guuugcacag      960

uucaucucuu ugaucgucgg cagcaaaggg aacuuucaaa agccauucga ccugauugaa     1020

aaguccgaua uagagugugc uaaagacuca uaugaagagg aucuagaaag ucuccuggcc     1080

cuuaucggag acgaguacgc ggagcuguuc guggccgcaa agaaugcuua uagcgccgug     1140

gucuugucuu ccauuaucac cguugcugaa acggagacua acgccaaacu cucagcaagc     1200

augauagaaa gguuugauac ccaugaggaa gaccugggcg aguuaaaggc cuucauuaaa     1260

cugcaccucc ccaagcacua cgaggaaauc uuuaguaaua cagagaagca ugguuaugcu     1320

ggguacaucg auggaaaaac uaagcaggcg gacuucuaca aauauaugaa gaugacccuu     1380

gaaaacauug agggcgcaga uuacuuuauc gccaaaauug agaaggaaaa cuuccugcgg     1440

aagcagcgaa cauuugacaa uggcgcuaua ccucaccagu ugcaucuaga ggaacuggag     1500

gccauccucc accaacaggc aaaauauuac ccauuccuga aggaaaacua ugauaaaauu     1560

aagucucuug ugaccuuucg cauccccuac uucguagggc cucuggccaa uggacagucc     1620

gaguuugcuu gguugacgag aaaagccgac ggugagauca ggccauggaa cauugaagag     1680

aagguggacu ucggcaaaag cgcagucgau uuuauagaaa agaugacuaa uaaggacaca     1740

uaucucccga aagagaacgu gcugcccaag cacucguuau gcuaccaaaa auaccugguu     1800

uauaaugaac uaaccaaggu gcgguacauc aacgaucagg ggaaaacuuc uuauuucucc     1860

ggacaggaga aggagcagau uuucaaugac cucuuuaagc aaaaacgcaa ggucaaaaag     1920

aaagaucugg aacuuuucuu gagaaacaug ucacaugugg agaguccuac aaucgaaggc     1980

cuggaggaca gcuuuaauuc cucuuacagc accuaucacg aucuccugaa gguagguauu     2040

aagcaggaaa uccuugacaa cccaguuaau acggagaugc uggagaacau agugaaaauc     2100

uugaccgucu ucgaagauaa gaggaugauu aaagagcagc uccagcaauu uucagacgug     2160

cuggaugggg ucguguuaaa gaaacuggaa cgucggcauu acacaggcug gggacgacua     2220

agugcgaagc uccugauggg caucagagac aagcaguccc accuuacuau uuuggauuau     2280

cugaugaaug acgacgggcu caacaggaac cugaugcagu uaaucaauga uagcaaccug     2340

ucuuucaaau caauaauuga gaaggagcag guuaccacag cugacaaaga uauccaaagu     2400

auuguggccg accuugcagg aagccccgcc aucaagaaag guauauugca gucccucaag     2460

aucguagaug aacuggucuc ugugaugggc uacccuccac agacuauugu uguggagaug     2520

gcucgcgaaa aucagaccac agggaaagga aagaacaauu cgcggcccag auacaagagc     2580

cuagagaaag ccaucaagga auuuggcucc caaauucuga aagagcaucc gaccgacaac     2640

caggagcuca ggaauaaccg ccuguaucuu uacuauuugc agaaugguaa ggauauguac     2700

acugggcaag accuggauau ccacaacuua ucaaauuaug acauagauca cauugucccu     2760

cagaguuuca ucacggacaa cucuaucgau aaucucgugc ugacaagcuc cgcaggcaac     2820

cgagaaaaag gagacgacgu acccccacuu gagauugugc ggaagcguaa ggucuuuugg     2880

gaaaaacugu accagggcaa uuugauguca aagagaaaau ucgauuaucu caccaaggcu     2940

gagagagggg gacugacuga agccgacaaa gcgcgcuuua uccaucggca gcuaguugag     3000

accagacaaa uuacaaagaa cguggccaac auacugcacc agagguucaa uuacgagaag     3060

gaugaccaug guaacaccau gaaacaggug cgaaucguca cucucaagag cgcucugguu     3120

ucucaguuuc gcaaacaauu ccagcuuuac aaggugcggg augugaauga cuaucaccac     3180

gcacaugaug ccuacuugaa cggcguaguc gcuaauacac uguuaaaagu guauccucag     3240

cucgaacccg aguucguuua cggggacuau caccaguuug auugguucaa ggccaacaag     3300

gcaacggcga aaaagcaauu uuacaccaau auuaugcugu ucuuugccca gaaagacaga     3360

aucauugaug aaaacggaga gauccuuugg gacaagaaau aucuggacac ugugaagaag     3420

gucauguccu acaggcagau gaauauagug aaaaagacag aaauccaaaa aggcgaguuc     3480

aguaaggcua ccauuaaacc aaaggguaac agcucuaaac uaaucccccg uaagacaaau     3540

ugggauccua ugaaguacgg gggccucgac uccccaaaca uggccuaugc aguugugauu     3600

gaguacgcua aaggaaagaa uaaacuggua uuugaaaaga aaaucauacg cgucacuauu     3660

auggagcgga aggccuucga aaaggaugag aaagcauuuu uggaggaaca gggcuauaga     3720

cagccgaagg ugcuggccaa auuacccaag uacacccucu augaguguga agaggggagg     3780

cgacggaugc uggcuucagc gaacgaagcc cagaaaggaa aucaacaggu gcuuccuaac     3840

caucugguca cguugcucca ccaugcagcu aacugcgagg uuagugacgg caagagccug     3900

gauuacaucg agucgaaucg cgaaauguuc gccgagcuac uggcccacgu guccgaauuu     3960

gcuaagagau auacccuugc agaggccaac cucaauaaaa ucaaccagcu guucgaacag     4020

aauaaggagg gugacauuaa agcgauagcu caaucuuucg uagauuugau ggccuuuaac     4080

gcaauggggg ccccagcuag cuucaaguuu uucgagacaa cuaucgaaag gaaacgguac     4140

aauaaccuga aggaguuacu caauucaacc auuaucuacc agaguauuac aggacuguau     4200

gaaucccgaa agagacuuga cgauaaacgc cccgcagcca cuaagaaagc gggccaggcu     4260

aagaaaaaga aauaaggacu agucccuguu cccagagccc acuuuuuuuu cuuuuuuuga     4320

aauaaaauag ccugucuuuc agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                4415


<210>  3642
<211>  4448
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3642
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagaa accauacagc      240

auuggacugg acauagguac caacuccguu ggcugggccg ugguaacaga ugacuauaag      300

gugccggcga agaaaaugaa gguccucggg aauacugaua aaucucauau cgagaagaac      360

cuucugggag cuuuguuauu cgacucaggc aauacggccg aagauaggcg gcugaaacgc      420

accgcacgac guagauacac aaggcggcgc aaccggauuc uauaucucca ggagaucuuu      480

agugaagaga uggguaaggu ggacgauagc uucuuucaca ggcuggaaga cuccuuccuu      540

guuacugagg auaagagagg ggagcgccau cccauuuuug gaaaucugga agaggaagug      600

aaauaccacg agaacuuccc uaccaucuau cauuugcgac aauaccucgc cgacaaucca      660

gagaaggucg aucugagacu gguguaucuc gcuuuagcac acauaaucaa auuuaggggc      720

cacuuccuga uugaaggcaa guuugacaca cggaacaaug auguacagcg ucuuuuccag      780

gaguuuuugg ccguuuacga caacaccuuc gaaaauucuu cgcugcagga gcaaaacgug      840

caggucgaag agauccuaac ugacaaaauu ucaaagagcg cuaaaaagga ucgcgugcuc      900

aagcuguuuc ccaaugagaa aaguaacggg agauucgccg aguuccuuaa gcugaucgug      960

ggaaaucagg cggacuuuaa aaagcauuuc gaguuggaag agaaagcacc ucuccaguuu     1020

uccaaggaua cauacgaaga ggagcuggaa gucuuacugg cccaaauagg ugacaacuau     1080

gcugaacucu uccuuucugc caagaaacug uacgauagca uuuugcuauc cggcauccug     1140

accguuacgg acguggggac uaaggcacca cucucagcua guaugauuca gagguauaac     1200

gaacaccaga uggaucuggc ccagcuuaaa caauuuaucc ggcagaagcu gagcgacaaa     1260

uacaaugagg uauucucuga uguguccaag gacggauaug cgggcuacau cgaugguaag     1320

accaaccagg aagccuuuua uaaauacuug aaggggcucc ugaauaaaau ugagggauca     1380

ggcuacuucu uagacaagau agagcgagaa gacuuucugc gcaaacaaag aacauucgau     1440

aacggcagca ucccccauca gauucaccua caggagauga gggcaaucau acggcgccag     1500

gcugaauuuu auccuuuccu cgccgacaau caagauagaa uugagaagcu gcuuacuuuu     1560

aggaucccau acuaugucgg gccguuggcu cguggaaaga gugacuucgc cuggcugucu     1620

cggaaauccg cagauaagau cacccccugg aacuucgacg agauugugga uaaagaaagc     1680

ucggccgagg cuuuuaucaa ucgaaugaca aacuacgacc ucuaucugcc uaaucagaag     1740

guucuuccaa aacacucucu guuguacgaa aaguucaccg uguauaacga gcucacgaaa     1800

gucaaguaca agacugaaca ggguaaaaca gcguuuuucg augcaaauau gaagcaggag     1860

auuuuugacg gcguguucaa aguauacaga aagguuacca aagauaagcu gauggacuuu     1920

uuagagaagg aguucgauga auuuaggaua guggaccuga cugggcuaga caaagaaaac     1980

aaggucuuca augccuccua uggaacauac caugaucucu gcaaaauccu ggacaaggau     2040

uuucuugaca acaguaaaaa ugagaagauu uuggaagaua ucgugcugac ccucacgcug     2100

uucgaggacc gcgagaugau ccggaagaga uuagaaaacu auucagaucu gcuuaccaaa     2160

gagcaaguca agaaauugga aaggcgccac uacacaggcu ggggucgacu cagcgcugag     2220

cugauucaug ggauacggaa caaggaaucc cguaaaacua uccuagacua ucugauugau     2280

gacggcaauu cuaacagaaa uuuuaugcag cucaucaacg augacgcccu gagcuucaag     2340

gaggagauug caaaggccca ggugaucgga gaaaccgaua aucuuaacca gguuguguca     2400

gacauagcug gcagucccgc caucaaaaag gggauuuugc aaucccugaa aaucguagac     2460

gaguuaguca agauuauggg acaccagccu gaaaauaucg ugguugagau ggcaagggag     2520

aaccaguuca caaaucaggg ucgccggaac agccaacaga gacucaaagg ccugacugau     2580

ucuauaaagg aauuuggguc acagauucuu aaagagcauc caguggaaaa uagucaacug     2640

cagaacgaca gguuguuccu cuacuaucug cagaauggac gagauaugua caccggcgag     2700

gaacuagaca ucgauuaccu gagccaguau gacauugauc acaucauacc ccaagcguuu     2760

aucaaggaca acuccauuga uaaucgcguc cucacaucuu cgaaggagaa ccgggguaaa     2820

agcgacgaug ugccguccaa ggacguagug agaaaaauga agucauacug gaguaaacug     2880

cuuucugcua aguugaucac ccagaggaag uucgacaacc ugacuaaagc cgagcguggg     2940

ggcuuaacgg augacgauaa ggcaggauuu auuaaacgcc agcucguuga aacacggcag     3000

aucaccaagc acgucgccag aauacuggac gagagguuca auacugaaac cgaugagaac     3060

aauaaaaaga uucgacaagu gaagaucgug acacuuaaaa gcaaccuggu cuccaauuuu     3120

cggaaggagu ucgaacuaua uaaaguucgc gagaucaacg acuaccauca cgcucaugau     3180

gccuaucuca augcagugau uggcaaggcu cuguuggggg uguacccuca gcuggaaccc     3240

gaguuuguau auggagacua cccacacuuc cacggucaua aagaaaacaa ggccaccgcg     3300

aagaaauuuu ucuacucaaa uaucaugaac uucuuuaaga aagaugacgu cagaacugau     3360

aagaauggcg agauuauaug gaaaaaggac gaacacauca gcaacauuaa gaaaguguua     3420

ucuuauccuc agguuaauau cgugaagaaa gucgaggagc agacaggggg auucuccaag     3480

gaaaguauuc uccccaaagg caacagcgau aagcugaucc caaggaaaac gaagaaguuu     3540

uacugggaca ccaaaaagua ugguggguuc gacucuccca uaguggccua cuccauccuu     3600

guuauugcug auaucgagaa aggcaagagu aaaaagcuga agacugugaa agcauuggua     3660

ggagucacaa uuauggaaaa gaugaccuuu gagcgggacc cuguggccuu ccucgagcga     3720

aaaggcuaua gaaaugugca agaagagaac aucauaaagc ugccaaaaua cucacuauuu     3780

aagcuggaaa acgggcgcaa gaggcuucuc gcuagcgccc gugagcugca gaaaggaaau     3840

gaaauugucu ugccgaacca ucugggcaca uuacucuauc acgcaaagaa uauccauaaa     3900

guugaugagc ccaagcaccu ggacuacgug gauaaacaca aggacgaguu caaggaacuu     3960

cuggauguag ugucgaacuu uuccaaaaag uacacuuugg cggaggguaa ucucgagaaa     4020

aucaaggaac uguaugccca gaacaauggg gaagaccuaa aagagcuggc uucuagcuuc     4080

auuaaccuuc ucaccuuuac ggccauagga gcuccugcaa ccuucaaguu uuucgauaaa     4140

aauaucgacc ggaagagaua cacaucaacu accgagauuc ugaacgccac auugauccau     4200

caaaguauua cuggcuuaua ugaaaccagg aucgaucuga auaagcucgg uggcgacaaa     4260

cgcccagcag ccacgaagaa agcugggcag gcgaagaaaa agaaguaagg acuagucccu     4320

guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaagaauu                                                              4448


<210>  3643
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3643
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcacagac aacaaaccug      240

agcuacauuc ucggacuuga ccuggguaua gccuccguug gcugggcggu gguagagauc      300

aaugaaaacg aggauccaau uggguugauc gacgugggag ucaggauuuu cgaacgggcu      360

gaggugccga agacuggcga aucuuuagcc cugucacgcc gacuagcacg uaguacgaga      420

aggcucaucc ggcgcagagc ccauaggcug cuucuggcua aacgguuuuu gaagcgcgag      480

gguauacuca gcaccaucga ucuggagaag gggcugccca aucaagcaug ggaacuccga      540

guugccggau uagagagaag gcuguccgcu auugaauggg gcgccgugcu uuugcaccug      600

aucaaacauc ggggcuaucu aucuaagcgu aaaaacgagu cgcagacaaa uaacaaggag      660

cucggggcgc ugcuuucagg agucgcacag aaucaccagc uguugcaaag cgacgauuac      720

cgcacuccug ccgaacucgc ucugaaaaag uucgccaagg aggaagguca uauuagaaac      780

cagaggggcg cauauaccca cacauuuaau cgguuagacc ugcucgcuga gcuuaaccug      840

uuguucgccc agcagcacca auuugggaau ccacauugca aagaacacau ccagcaguac      900

augaccgagc uacugaugug gcagaagccc gcgcucagug gagaggccau acugaaaaug      960

cuuggcaagu guacucauga gaaaaacgag uucaaggcag cuaaacacac auauuccgcc     1020

gaacgauuug uguggcugac caaguugaau aaccuccgca uucuggagga uggugcugaa     1080

agagccuuaa augaggagga aaggcaacug cuaaucaacc acccuuacga gaagucuaaa     1140

cucacguaug cacagguacg gaagcugcuu ggguugagcg aacaggccau uuucaaacau     1200

cugcgcuacu ccaaggagaa cgcugaauca gcgacuuuua uggagcucaa agcauggcac     1260

gccaucagaa aggcucugga gaaucaagga cuuaaggaca ccuggcagga ucuggccaaa     1320

aagccagacu ugcucgauga aaucggcaca gcauucaguc uguacaaaac ugacgaggau     1380

auucagcagu auuuaaccaa caagguuccc aauagcguga uaaacgcccu gcuagucucu     1440

cucaauuuug acaaauucau cgaacugucc cuuaagucau ugaggaagau ucugccucuc     1500

auggagcaag gcaaacguua cgaccaggcu ugccgggaga ucuaugggca ucacuacgga     1560

gaagccaacc agaagacaag ccagcuguua ccagcaauac cggcgcaaga gauucgaaau     1620

cccguggugc ugagaacccu uagucaggcu aggaaaguca ucaacgccau cauucgccag     1680

uaugguucuc cugcacgggu ucauaucgaa acgggcagag aguuggggaa guccuuuaaa     1740

gaaaggcgcg agauucagaa gcaacaggag gauaaucgaa cuaagcggga aagcgccgug     1800

cagaaauuca aggaacucuu cucggacuuu ucuuccgaac caaaaucaaa ggauauacug     1860

aaauuccguc uauacgagca gcaacacgga aagugucugu auaguggcaa agaaaucaac     1920

auucacagac ucaaugagaa ggguuacgua gagaucgacc augcucugcc cuuuagcagg     1980

acaugggaug acuccuucaa caauaaggug cuugucuugg ccucugaaaa ccagaauaaa     2040

gggaaccaga ccccuuacga guggcugcaa ggcaagauca acagcgaacg cuggaaaaau     2100

uuuguggcau uaguucucgg aucacagugc agugcugcca agaaacagcg gcugcuuacu     2160

caggugauug augacaacaa guucauagau agaaaucuga acgacacaag guauaucgcg     2220

cgauuuuugu ccaauuacau ucaagagaac cuccugcuag ucggcaagaa uaaaaagaac     2280

guguucaccc caaaugggca gaucacggcc cugcuccgca gccggugggg acugauuaaa     2340

gcuagagaaa acaauaacag gcaccaugca cuugaugcca ucguaguugc uugugccacc     2400

cccucuaugc agcagaagau aacacguuuu auccgcuuca aagaggugca cccguauaag     2460

auugagaauc gguacgaaau ggucgaccaa gagucaggug aaaucauuag uccucacuuu     2520

cccgagccau gggcauauuu cagacaggag gugaacauca gggucuuuga caaucauccu     2580

gauacugugu ugaaggaaau gcugcccgac cgaccacagg cgaaccacca guucguucaa     2640

cccuuauucg ugagccgggc cccuacccgc aaaauguccg gccaggggca uauggagaca     2700

auaaagucug cuaaaagacu cgccgaagga auuucgguac ugaggauccc acuuacucag     2760

cugaagccga accuacucga gaauaugguc aacaaagaac gggagcccgc acuguacgcu     2820

ggcuugaagg cccgacuggc agaguuuaau caagauccug ccaaggcuuu cgcgacccca     2880

uuuuauaaac agggugggca acaggugaag gccauuagag uugaacaggu gcagaaaagc     2940

ggcgucuuag ugcgcgagaa caauggagua gcagacaacg cuuccaucgu gaggacagau     3000

guuuucauaa agaauaacaa auuuuuccuc guccccaucu acaccuggca aguggccaag     3060

ggcauucugc cuaauaaagc caucguggcu cacaagaacg aagacgagug ggaagagaug     3120

gaugaggggg caaaguuuaa auucucacuu uuuccaaaug accuggucga auugaagacu     3180

aaaaaggagu acuucuuugg auauuacauu ggucucgauc gugccacggg caacaucagu     3240

cugaaagaac augacgggga gauaucuaag ggaaaggaug gcguuuaucg ggugggugug     3300

aaacuagcgc ugagcuucga aaaguaccag guagacgagc uugggaaaaa uagacagauu     3360

ugcaggcccc agcagcgcca gccgguccga aagagaccug cugccaccaa aaaggcaggc     3420

caagccaaga aaaagaaaua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3480

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  3644
<211>  3659
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3644
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagccgc guucaagcca      240

aacccgauua auuacauacu gggacucgac aucgguauug cuagcguugg cugggccaug      300

guggagaucg augaagacga gaaccccauu ugccuuaucg aucugggggu aaggguguuu      360

gaacgggcag aggucccuaa aaccggagac uccuuggcca uggcucgccg auuagcacgu      420

ucugugagaa ggcugacacg gcgcagagcc cauaggcuac uccgggcucg ccgacugcuu      480

aagagagaag gcguucugca ggccgcggau uucgacgaga augguuugau aaagucacuc      540

ccaaacacuc ccuggcaacu gagggcagcc gcucuggauc ggaaacucac gccuuuagag      600

uggagugccg ugcugcuuca cuugaucaag caucgugggu aucugagcca gcgcaaaaau      660

gaaggagaga ccgcagacaa ggaacuaggc gcucuccuga aaggcgucgc cgauaacgcg      720

cacgcccuuc agacagggga cuuuagaacu ccagcagagc uggcuuugaa uaaguucgag      780

aaggaauccg gacauauuag gaaccagcgg ggugauuacu cucacaccuu uucgcgaaaa      840

gaccuccaag ccgagcugau cuuacuguuc gaaaagcaga aagaguuugg caauccccac      900

gugucagggg gacucaagga aggcauugag acacuucuga ugacccagcg cccugcuuug      960

agcggugaug ccguacagaa aaugcuaggg cauuguacuu ucgagccagc agaaccgaag     1020

gccgcuaaaa acacauauac cgcggagaga uuuaucuggc ugacgaagcu caauaaccug     1080

aggauacuug aacaaggaag ugagcggccc cugacugaca ccgaacgcgc aacauugaug     1140

gacgagccuu acagaaaguc caaacucacu uaugcccagg cuaggaagcu guuaggccug     1200

gaggauaccg ccuucuuuaa aggccuacgu uacgggaagg acaaugcaga agccucuaca     1260

cucauggaga ugaaggcuua ucacgccauu agccgggcac uggaaaagga gggacuuaag     1320

gauaaaaagu ccccauugaa ccugucaccc gaacuccagg acgagaucgg uaccgcguuc     1380

agucuguuua aaacggauga ggacauuacu ggccgacuua aggauagaau ccagccugaa     1440

auccuggagg cuuugcucaa acauauuagc uucgacaagu ucguucaaau aucucugaag     1500

gccuuaaggc gcaucgugcc acugauggaa caggggaaac gguacgauga ggcaugcgcc     1560

gagauuuacg gagaccacua uggcaagaaa aauacagaag agaagaucua ccuacccccg     1620

auaccugcug augaaauuag aaaccccguc gugcucaggg cccuguccca ggcacgcaaa     1680

gugaucaacg gugucguucg acgguauggg ucaccagcuc guauccacau ugagaccgcc     1740

agagaagugg gcaagagcuu uaaggacagg aaagagaucg agaagcgcca agaagagaau     1800

cggaaagaca gagaaaaggc ggccgcuaaa uucagggagu acuuuccuaa cuucguagga     1860

gaacccaaga guaaagauau ucuuaaguug cgacuguaug agcagcagca uggcaagugu     1920

cucuacucug ggaaagagau aaaucuggga cgcuuaaacg aaaaggguua uguggagauc     1980

gaccacgcac ugccauuuuc ccggacuugg gaugacagcu ucaauaacaa aguccuugug     2040

uugggcucgg aaaaucagaa caaggggaau caaacacccu acgaguacuu uaacggaaaa     2100

gauaauucua gagaauggca ggaguucaag gccaggguug agaccucccg uuuuccucgc     2160

ucaaagaaac agcggauucu ccugcagaag uucgacgaag auggcuuuaa agagagaaac     2220

cuaaaugaca cgagguaugu gaaccgauuc cugugccaau uugucgcuga ucggaugcgc     2280

cucaccggua aggggaaaaa gagaguguuc gccaguaaug gccagaucac aaaccugcuu     2340

aggggauucu ggggcuugcg gaagguacga gcagaaaacg acagacauca cgcgcuggau     2400

gccguugugg ucgcuuguag cacuguggcc augcagcaga aaaucacccg cuuugucagg     2460

uacaaggaga ugaaugcauu cgacgggaaa acaauugaua aggagacugg agaaguguua     2520

caucagaaaa cccacuuucc acagccgugg gaguucuuug cucaggaagu uaugauacgu     2580

guguucggua agcccgacgg caagccugag uuugaagagg ccgacacacc agagaaacuc     2640

cggacccugc uugcagaaaa gcuguccucu agacccgagg ccguacacga auaugucacu     2700

ccuuuguucg ugagcagggc uccaaaccgc aaaaugucag ggcagggaca uauggagacg     2760

guuaagagug cgaaacgacu cgaugaaggc guguccgucc ugagagugcc ccuaacacaa     2820

cugaagcuca aagaccugga gaagauggua aaucgggaga gggaaccgaa acuuuacgag     2880

gccuugaaag cacgccugga agcucacaag gaugacccug ccaaagccuu ugcugagcca     2940

uucuauaagu acgauaaagc agguaaccgu acccaacagg ugaaggccgu uagagucgaa     3000

caagugcaga agacuggggu guggguccgg aaucauaacg gcaucgcgga caaugcuacc     3060

augguuaggg uggauguguu ugagaaagga gacaaguauu acuuaguacc cauuuacagc     3120

uggcaggucg ccaaaggcau ccucccugau cgcgcagugg uucaggggaa ggacgaggaa     3180

gauuggcaac ugauugacga uucuuucaac uuuaaauucu cacuucaccc caaugaccug     3240

guggagguca ucacaaagaa ggcccgaaug uucggauauu uugcuaguug ccacagaggc     3300

accgguaaca uaaauauccg gauucaugac cuagaucaca aaaucgggaa gaacggcauu     3360

cucgaaggaa ucggugugaa aacugcacug agcuuccaga aguaccagau agacgaguug     3420

ggcaaagaga uuaggccaug ucgccugaag aagagaccuc ccguuaggaa acggccagcc     3480

gcgacaaaga aagcugggca ggccaagaaa aagaaguaag gacuaguccc uguucccaga     3540

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      3659


<210>  3645
<211>  3665
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3645
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggca      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaagua ccacguugga      240

auugacgugg guaccuucag cguaggccug gccgcgauag agguggauga cgcugggaug      300

ccaaucaaaa cacucucccu ugucucucac auucaugauu caggacugga cccggaugaa      360

aucaagagug ccgugacuag guuggcaagc uccggcauug cccggcgcac gcgacguuua      420

uauagaagga agcggcgcag acugcagcaa cuagacaaau uuauccagag gcaggguugg      480

cccguuauag agcucgaaga uuacucugac ccucuguauc cauggaaggu gcgggcugag      540

cuugcagccu cguacaucgc ugaugaaaaa gagcgcgggg agaagcuguc agucgccuug      600

cgacacauug cgagacauag gggauggcgg aaccccuaug caaaagugag cagucucuac      660

cugccugacg gcccauccga ugccuucaag gcuauccgug aagagauuaa gcgcgccucu      720

ggccagcccg uaccugaaac cgcaacaguu gggcaaaugg ugacucugug cgagcucgga      780

accuuaaaac ugagagguga gggcgggguc cuuagcgcua gguugcagca guccgacuau      840

gcccgggaaa uccaggagau augucgaaug caagaaauug gacaggagcu guaccgcaag      900

aucauugaug ugguguuugc ggccgaauca ccaaaaggca gugcaagcuc uagagucggu      960

aaggacccgc uacagcccgg gaaaaauagg gcucucaagg ccuccgacgc uuuccagcgg     1020

uaccgcaucg ccgcacugau cggaaaccuu agaguuaggg uggauggcga gaaacguauu     1080

cugucaguag aggaaaagaa uuugguguuu gaccaccucg ucaaccugac accuaagaaa     1140

gagccagaau gggugaccau agccgagauc uuaggcauug aucgggggca acugaucgga     1200

acugcuacaa ugaccgacga uggugaacga gcgggcgcaa gacccccuac gcacgacacu     1260

aauaggagca uaguuaacag ucgcauugcc ccacucgugg auugguggaa gaccgcuucu     1320

gcccuugagc agcaugcaau ggucaaagcc cuguccaaug cugaggugga cgauuucgac     1380

agccccgaag gggccaaggu acaggcauuu uucgcggauu uggacgacga uguucacgcu     1440

aaacuagacu cgcugcaucu cccgguggga cgggccgcau auucugagga uacacugguc     1500

agacuuacua ggcgcaugcu guccgacggc guggauuugu acaccgcccg acuccaagaa     1560

uuugguaucg agccuucaug gacaccccca accccucgga ucggggaacc cgucggcaac     1620

ccagcugugg accguguucu gaagacggug aguagauggu uagagagcgc cacuaagaca     1680

uggggagcac ccgagagggu aauuaucgaa cacguccgcg agggcuucgu gaccgaaaaa     1740

cgggcuagag agauggaugg ggacaugagg cgacgcgccg cgcggaaugc caagcuguuu     1800

caggagaugc aggaaaaacu aaacguucag ggaaagccuu ccagagcuga ucucuggagg     1860

uaucaaucug ugcagcguca gaauugccag ugugcauacu gcgguagccc aauuacuuuc     1920

ucaaacagug agauggacca cauagucccg cgcgccggcc aaggguccac aaauacccgg     1980

gaaaaccugg uggcuguaug ucauagaugc aaucagagca aaggaaacac gcccuuugcc     2040

aucugggcaa agaacaccuc uauugagggc gugucaguua aggaagcggu cgagaggaca     2100

cgacacuggg ugacugauac cgguaugcgg aguacagacu ucaaaaaguu cacuaaagcc     2160

guggucgagc gcuuucagag agcuaccaug gaugaagaga ucgacgccag gagcauggaa     2220

uccguugcau ggauggcuaa ugagcuucgg ucucgagugg cccagcauuu cgcaucgcac     2280

gggacaaccg ugagaguaua ucgcggcagc uugacugccg aagcuaggcg ugcguccgga     2340

aucucaggca agcugaaauu uuucgacggg gucggaaaga gucggcucga uagaaggcau     2400

cacgccauug acgcagcugu gauagccuuu acgucugauu acguugccga gacacuggcu     2460

gugcgcagca accuuaagca aucccaggca caccgacagg aggccccuca guggagagag     2520

uucaccggua aagacgcgga acaucgggcu gccuggaggg ucugguguca aaagauggaa     2580

aaacugucag cauugcucac ugaggaucug cgcgacgauc gugugguugu gaugagcaau     2640

guaagauuac ggcugggcaa cgggucugcc cacaaggaaa ccaucggaaa acuauccaag     2700

gucaaacuca guagccagcu gucugugucc gacauugaua aggcuucaag ugaggcacuu     2760

uggugcgccu ugacaaggga gccaggcuuu gaccccaagg aaggucugcc ugcgaaucca     2820

gagcgccaua uccgagugaa cgggacccac gucuaugcug gcgauaauau uggacucuuc     2880

cccguuagcg ccggcucgau cgcacugaga gggggauacg ccgaauuagg cuccucuuuu     2940

caccaugcuc ggguguacaa aauaacuagc gguaagaaac cggccuucgc aaugcugagg     3000

guauauacaa ucgaccuuuu gccuuaccgc aaccaggauc ucuuuucagu ggagcugaag     3060

ccacaaacga ugaguaugag acaggcugag aaaaagcuaa gggacgcccu ggcgaccggg     3120

aaugccgaau aucucggaug gcuggucgug gacgaugagc uuguugugga cacuuccaag     3180

auugcaacag aucaggucaa agcuguggaa gccgaguugg gcaccauccg gcguuggcgc     3240

guugacgguu ucuuuucucc cagcaagcug cgauuaagac cucuccagau guccaaagaa     3300

ggcauuaaga aagagucagc ucccgagcug agcaagauca uagauaggcc aggguggcuu     3360

ccugcaguga acaagcuguu cagugacgga aaugucacag uagugcggcg cgauucuuug     3420

ggcagaguua ggcucgaauc cacugcccau cugcccguga ccuggaaagu ccaaaagcgg     3480

ccagccgcua cgaaaaaggc ggggcaggcc aaaaagaaga aauaaggacu agucccuguu     3540

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

gaauu                                                                 3665


<210>  3646
<211>  3365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3646
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcagccag gauucuggcg      240

uucgacauag gaaucagcuc cauugguugg gcuuuuucug agaacgauga acucaaggac      300

ugcggcguuc ggaucuucac caaaguggag aauccaaaga caggggaauc acuugcccug      360

ccgcgccgau uggcacguag ugccagaaag agguuagcuc ggcgcaaagc aagacugaac      420

caucuaaagc accucauugc caaugaguuu aaacugaacu acgaagauua ucagagcuuc      480

gacgaguccc uugcuaaggc cuacaaagga ucucugaucu cgcccuauga guugagguuu      540

cgggcgcuca augaacugcu gucaaagcaa gauuucgcac gcguaauacu ccauaucgcc      600

aagcgaagag gcuacgacga uauuaaaaac agcgacgaua aggagaaagg ugcuaucuua      660

aaggccauua aacagaauga agagaagcug gcaaacuauc agaguguggg ggaguaccuu      720

uauaaagaau acuuucagaa guucaaggaa aauuccaaag aauuuacuaa cgucaggaau      780

aagaaagagu cuuacgaacg guguaucgcu cagagcuucu ugaaggacga gcugaaacua      840

auauuuaaga agcaacguga guucggauuu uccuucucua aaaaguucga agaggaagug      900

cucaguguug ccuuuuauaa acgcgcgcug aaggauuuca gccaccuugu gggcaacugc      960

ucauuuuuca cggacgagaa aagagccccu aaaaauuccc cacuggcauu uauguucguc     1020

gcuuugacca ggauuaucaa ccuccugaau aacuuaaaga acacagaagg cauucuguac     1080

acuaaagaug accucaaugc ccuucugaac gagguguuga agaaugggac ccuaacauau     1140

aaacagacca agaaacugcu cggacuguca gacgauuacg aguuuaaggg ugaaaagggc     1200

acuuauuuca ucgaguuuaa aaaguacaaa gaguucauca aggcucuugg ggagcauaac     1260

cugagccagg acgauuugaa ugaaauugcc aaagacauaa cacucaucaa ggaugagauu     1320

aaacugaaga aggcauuagc caaauaugac cugaaccaaa aucagaucga uagucuaucu     1380

aagcucgagu uuaaagacca ccugaacaua uccuucaagg cucuuaaauu gguaacuccc     1440

cugaugcucg aaggaaagaa guacgaugag gcguguaaug aacugaaccu uaaaguugca     1500

auuaaugagg acaagaaaga uuuucugccu gccuucaacg agacguacua uaaggacgaa     1560

gugacuaauc cagucguguu gcgggcuauc aaagaguacc gaaaggugcu caacgcccug     1620

uuaaagaaau auggcaaggu ccacaaaauc aauauugaac uggcacgcga gguugguaag     1680

aaccauagcc agagagccaa aaucgaaaag gagcagaacg agaauuacaa ggcuaaaaag     1740

gacgccgaac uagagugcga aaaacucggg cugaagauua acucgaaaaa uauacuuaag     1800

uugaggcugu ucaaagagca aaaggaauuu ugugcauauu cuggagagaa gaucaaaauu     1860

uccgaucucc aggacgagaa gaugcuggaa aucgaucaca ucuaccccua uucacggagu     1920

uucgacgaua gcuacaugaa caaaguguua guauuuacca agcagaauca agagaaacug     1980

aaccagacac cuuucgaagc guuuggcaau gacuccgcua aguggcagaa gauugaggug     2040

cuugccaaaa acuugccaac uaagaaacag aagcgcauac ucgauaaaaa uuacaaggac     2100

aaggaacaaa agaacuucaa ggauagaaau cugaacgaca ccagguauau cgcacgucua     2160

guccugaauu acacaaaaga uuaucucgac uuucugccgc uuucugauga cgagaacacc     2220

aaguugaaug acacgcagaa aggcagcaag gugcauguug aggccaaguc agggaugcug     2280

accagugcuu uacggcacac auggggauuc uccgccaaag aucgaaacaa ccaccuccau     2340

cacgcaauug acgcugugau cauugccuac gcgaauaaca gcaucgucaa ggccuuuucu     2400

gauuucaaaa aggaacagga gucaaauagu gcugaacugu augccaaaaa gauaagcgag     2460

cuugacuaca agaacaaaag aaaguuuuuc gagcccuuuu ccgguuucag gcagaaagug     2520

cuggauaaga ucgacgaaau uuucguaucu aaaccugagc gcaagaaacc aucgggcgca     2580

uugcaugaag agaccuuucg gaaggaagag gaguucuauc aaagcuacgg gggaaaggaa     2640

ggcguucuca aagcucugga gcuagguaag aucagaaaag ugaaugggaa gauugucaaa     2700

aacggcgaua uguuuagggu ggacaucuuc aagcacaaga aaacuaauaa guuuuacgcc     2760

guccccauau auacaaugga uuucgcacug aaagugcucc cuaacaaggc gguugcccgc     2820

uccaaaaagg gagaaauuaa ggacuggauc cugauggaug agaauuacga auuuugcuuc     2880

ucacuuuaua aagacaguuu gauucugauc cagaccaagg auaugcagga gccagaguuu     2940

guguacuaua acgcuuucac gucuagcacc guauccuuaa uagucucaaa acaugacaau     3000

aaguuugaaa cacucagcaa aaaccagaag auccuguuca agaaugccaa cgagaaagaa     3060

gugauugcaa agucuaucgg cauucaaaau cuuaaaguuu ucgagaagua caucgugucc     3120

gcucuggggg aagucacuaa agccgaguuu cgacagcggg aggacuucaa gaaaaagcgu     3180

cccgcagcca ccaagaaagc uggacaggcg aagaaaaaga aauaaggacu agucccuguu     3240

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3300

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3360

gaauu                                                                 3365


<210>  3647
<211>  3776
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3647
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaagcga ccugguucuc      240

ggacuugaua uugguauagg cuccgugggg guaggaaucc ugaacaaggu gaccggcgag      300

auuauccaua aaaauucuag gauuuuccca gccgcgcagg cugaaaacaa uuugguccgg      360

cgcacaaacc gacaaggucg uagauuagcc aggcggaaga agcaccgcag agugaggcug      420

aaucggcuau uugaggaauc agggcucauc acugacuuca cgaaaauaag uaucaaccug      480

aauccguacc agcuucgcgu uaagggacug accgaugagu ugagcaacga agaacucuuu      540

auugcacuga aaaauauggu gaagcaucga ggcaucuccu aucuggacga ugccucugac      600

gauggcaacu cgucagucgg ggacuacgcu cagauuguga aagagaauag caagcagcuc      660

gaaacaaaga cucccggaca aauccaguua gagagauauc agaccuacgg ucagcugagg      720

ggcgauuuca caguagaaaa agacgggaag aaacaccggc uuauaaacgu uuuuccuacc      780

agugcauauc guuccgaggc cuugcgcauu cugcaaacuc agcaggaguu caauccacag      840

aucacagaug aauuuauuaa cagauaccua gagauccuca ccggaaagag gaaauauuac      900

cauggccccg guaaugaaaa gucucggacg gacuacgggc gauaucgcac uagcggagag      960

acccuggaua acaucuucgg cauucuuaua ggcaaaugca cauuuuaccc ugacgaguuc     1020

agagcugcca aggcguccua uacugcacaa gaguuuaacc uguugaauga ccucaacaau     1080

cugaccgugc caacagagac caagaaauua ucaaaggaac agaaaaacca gaucauuaau     1140

uacgucaaga acgagaaagc cauggggccc gcuaagcugu ucaaguauau cgccaaacuc     1200

cuuaguugug auguggcaga cauaaaggga uacaggauug auaaaagcgg uaaggcugaa     1260

auccacacgu uugaggccua ucggaaaaug aagacucugg aaacauugga caucgagcaa     1320

auggaucgcg agacccuaga caagcuggcg uacgugcuca cucugaauac agaaagagag     1380

ggcauucagg aagcccuuga gcacgaguuc gcagaugggu cuuucuccca gaaacagguc     1440

gacgagcugg uucaauuuag gaaggcuaac ucaagcaucu ucggaaaagg cuggcauaau     1500

uuuaguguga aguugaugau ggagcucauu ccugaacugu acgagaccuc ugaagaacag     1560

augacgauau uaacccgucu ggguaaacag aagacaacuu ccagcucgaa caagaccaaa     1620

uauaucgaug agaagcuacu cacagaagag auuuacaauc caguaguggc caaaucuguc     1680

cggcaggcua ucaagaucgu gaacgccgca auuaaagaau auggggacuu cgauaauaua     1740

guuaucgaga uggcccgaga aacuaacgag gacgacgaga agaaagcuau ucaaaagauc     1800

cagaaggcga auaaagauga aaaggacgca gccaugcuga aagcugccaa ccaguacaac     1860

ggcaaggcag agcuuccgca cuccguguuu cauggacaca aacaguuggc caccaagauu     1920

agacuguggc accaacaggg cgaaaggugc cucuauacag ggaagaccau cucaauacau     1980

gaucugauca auaacaguaa ucaguucgag gucgaccaca uucuuccccu gagcaucacu     2040

uuugaugacu ccuuggcuaa caaagugcuc guauacgcca cggcaaauca ggaaaaggga     2100

caacgcacac cuuaucaggc gcuggauucu auggacgaug cuuggagcuu ccgggaguua     2160

aaagccuuug uuagagaguc aaagacccug aguaacaaaa agaaggaaua ccuacucacu     2220

gaggaagaca uuuccaaauu cgaugugagg aagaaauuua ucgagcgcaa ucuggucgac     2280

acccgauacg caagccgggu gguccuuaac gccuugcagg aacauuuccg ugcucacaag     2340

auagauacaa aagugucugu ugugagaggu caauuuaccu cacagcugag gcgccauugg     2400

ggcauugaga agacucggga cacauaucac caccaugccg uagacgcacu caucauugcu     2460

gccaguagcc agcugaauuu auggaagaaa cagaagaaca cgcuggucuc cuacucugag     2520

gaucaacuuu uggacaucga aaccggggag cucauaucgg augacgaaua uaaagagagc     2580

guguucaagg cgccauacca gcacuucguu gauacucuga aauccaagga guuugaagac     2640

ucaauccuau ucaguuauca gguggauucu aaauuuaaua gaaagauuag cgacgccaca     2700

aucuacgcua ccaggcaggc aaaggucgga aaagauaagg ccgacgagac auaugugcug     2760

ggcaaaauua aggauaucua cacucaagac gguuacgaug cuuucaugaa aauauauaag     2820

aaggacaaau ccaaguuucu cauguaccga caugaccccc agaccuucga aaaaguaauu     2880

gagccuaucc uggaaaacua uccaaauaag cagaucaacg agaaagggaa ggaggugccc     2940

uguaauccgu uucuuaagua caaagaggaa cacggcuaua uucgcaagua cagcaaaaag     3000

ggaaacggcc cugaaaucaa aucauugaag uauuacgauu cuaagcuggg gaaccacauu     3060

gacauaacgc ccaaagauuc caauaacaag guugucuuac agagugugag cccauggcgg     3120

gccgacgugu acuucaauaa aaccacagga aaguaugaga uccucggucu gaaauacgca     3180

gaucuucaau uugaaaaggg cacugggacc uauaagauuu cucaggagaa auacaacgac     3240

aucaagaaaa aggagggagu cgauuccgac ucagaguuca aauuuacacu guauaagaau     3300

gauuugcucc ugguuaaaga cacugaaacc aaggaacagc aacuauucag auuucugagu     3360

aggacgaugc cuaagcagaa acauuacgug gagcucaagc ccuaugauaa acagaaguuc     3420

gaaggcggug aggcgcugau uaaagugcuu gggaacguag ccaauagcgg ccagugcaag     3480

aagggauugg gcaaaucgaa caucuccaua uacaaggucc guacagacgu gcuggggaau     3540

caacacauca uuaaaaacga gggagacaag ccaaaauuag auuucaagcg ccccgcugcc     3600

accaagaaag caggccaggc uaagaaaaag aaauaaggac uagucccugu ucccagagcc     3660

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         3776


<210>  3648
<211>  4334
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3648
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaaccca guucgaggga      240

uuuacaaacc uguaccaagu uagcaagacu cucagguucg aacuuauucc acaggguaaa      300

acgcugaagc auauacagga gcagggcuuu aucgaagagg acaaggcccg gaaugaucac      360

uauaaagaau ugaagccgau uaucgaccgc auuuacaaaa ccuaugcgga ucaaugcuua      420

cagcuggugc agcuagacug ggagaaccuc uccgcugcca ucgauucuua ccgaaaggag      480

aaaacagaag agacucguaa ugcacugaua gaggaacagg ccaccuauag aaacgcuauc      540

caugacuacu ucauugggag gacagauaau cuuaccgacg caaucaacaa gcggcacgcc      600

gagauuuaua agggacuguu uaaagcugaa uuguucaaug gcaagguacu caaacaacug      660

gguacuguga caaccacgga gcaugaaaac gcccugcucc gcucauuuga uaaguucacu      720

accuacuuua guggguucua cgagaauagg aaaaacgucu uuagcgcgga agacaucucc      780

acagcaauac cccacagaau ugugcaggau aauuucccua aguuuaaaga gaacugucac      840

aucuucacuc gguuaauuac cgccguucca ucucugcgcg agcauuucga aaaugugaag      900

aaggcuaucg gaaucuuugu cucgacauca auugaggaag uguucagcuu ucccuucuau      960

aaccagcuuu ugacccagac gcaaauagac cuguacaauc agcuacucgg cggcaucagu     1020

cgagaggccg ggacugagaa aauuaaggga cugaacgaag uacuuaaccu ggcaauccag     1080

aaaaaugaug agacagcuca cauaauugcc uccuugccuc auagauuuau cccacucuuc     1140

aagcaaaucc ugucugacag gaacacccuu agcuuuauuc uggaagaguu caaauccgac     1200

gaagagguua uccagucauu uugcaaguau aagacucucc uucggaauga aaacgugcug     1260

gagacagcgg aggccuuguu caaugaacua aacaguauug aucugaccca cauauuuauc     1320

agccacaaaa agcucgagac gauuucuucc gcacugugug accauuggga uacccuucgu     1380

aaugcucugu acgaacgcag aaucucagag uugacaggua aaaucacuaa gagcgccaaa     1440

gagaaggucc agaggagucu caagcacgaa gacauuaacc ugcaggagau aaucucugcu     1500

gccggcaaag aauuauccga ggcauucaag cagaaaacca gcgaaauucu gucgcaugcc     1560

cacgcugcgc uagaucaacc ccucccuaca acucugaaga aacaggagga gaaggaaauc     1620

cuuaagucuc aguuggacuc ccugcucggg cuguaucauc uucuggauug guuugcagug     1680

gacgagucaa augaagugga uccagaguuc agugcccggu ugaccggaau uaaacucgaa     1740

auggagccga gccuguccuu cuacaacaag gcucgaaauu augccacuaa aaagcccuac     1800

ucugucgaga aauuuaaguu aaacuuccaa augccuaccc uggcaagcgg cugggacguu     1860

aauaaagaaa agaacaaugg ugccauccua uuugugaaga acgggcucua cuaucuggga     1920

auaaugccaa aacagaaggg ccgcuacaaa gcucuuucau ucgagcccac ugaaaagacg     1980

agugagggcu uugauaaaau guauuacgac uauuucccug acgccgcaaa gaugauccca     2040

aagugcucca cacaguugaa agcgguaacc gcucacuuuc agacucacac cacacccauu     2100

cugcucagca acaauuucau cgaaccgcug gagauuacca aggagaucua cgauuuaaac     2160

aauccugaaa aagagcccaa gaaauuucaa acugccuaug caaagaagac aggggaccag     2220

aaaggauaca gagaagcccu guguaagugg auagauuuca cgagggacuu ucuuucuaaa     2280

uacaccaaga cuacaucaau ugauuugagu agccuccggc cauccucuca guauaaagac     2340

cugggugagu acuaugcuga gcuaaacccu cugcucuacc auaucucguu ccagcgcauu     2400

gccgaaaagg agaucaugga ugcaguggaa accggcaagc uguaucuuuu ucaaauauac     2460

aauaaagacu ucgcuaaggg gcaccaugga aaacccaacu ugcacacacu guauuggacu     2520

ggcuuauuca gcccagagaa ucucgccaag accuccauca aacugaacgg ucaggcggaa     2580

uuauuuuaca gacccaaguc aaggaugaaa cguauggccc accggcuggg ggagaagaug     2640

uugaauaaga aacucaagga ucagaaaacg ccuauuccag acacccugua ccaagagcua     2700

uaugauuacg ucaaccaucg acugagucac gaccucucug augaagcuag agcacugcuu     2760

ccgaauguga ucacaaagga gguuagccau gaaauuauca aagacaggcg cuucacuucc     2820

gacaaguuuu ucuuucacgu gcccauaacc uugaacuauc aggccgcuaa uucaccuagc     2880

aaguucaacc agcgggucaa ugccuaccug aaagagcauc cagaaacacc cauuaucggc     2940

aucgauagag gagagaggaa cuuaauuuau aucacuguga uugacucuac cggcaagaua     3000

cucgagcagc gcucccugaa cacgauccaa caguuugauu accagaaaaa gcuggacaau     3060

cgagaaaaag agcggguagc agcgcgucag gccuggagug uuguggggac aauuaaggau     3120

cuuaagcaag gauaucuaag ccaggucauc cacgaaauug uggaccucau gauccacuac     3180

caggcugucg ugguucugga gaacuugaau uucgguuuua aaucuaagag aaccggcaua     3240

gccgaaaaag caguguacca gcaauucgag aagaugcuga ucgauaaauu aaacugccuc     3300

guacugaagg acuauccugc ugagaagguc gggggagugc uuaauccaua ccagcugacu     3360

gaucaguuua cauccuucgc caaaaugggc acccaaucag guuucuuguu uuauguuccc     3420

gcaccguaca caaguaagau ugacccucuc accggguucg uggauccauu ugucuggaaa     3480

acaaucaaga accaugaaag caggaaacac uuccuggagg gcuuugacuu ccuacauuau     3540

gaugugaaga cgggagacuu uauucugcac uucaagauga aucgcaaccu uucguuucag     3600

cggggccucc ccggguucau gccugccugg gacaucguau uugagaaaaa ugaaacccag     3660

uucgaugcua agggaacucc cuuuauagcg gguaaaagaa uugugccagu uaucgagaac     3720

cauagguuca caggccgaua ccgcgaccug uauccugcca augaauugau cgcacuguua     3780

gaggaaaagg ggauugucuu ccgggaugga uccaacauac uccccaaacu gcuugagaau     3840

gacgauucuc acgcuaucga caccauggug gcccugauua gaagcguguu gcagaugagg     3900

aacucaaaug ccgcuacagg cgaagauuac aucaacaguc caguccguga ccucaacggu     3960

guuuguuuug auucccgcuu ccaaaauccg gaguggccua uggacgcaga ugccaacggg     4020

gcguaccaca uugcucugaa gggccagcua cugcuuaauc aucucaaaga gucuaaggac     4080

cugaaguugc agaacggaau cagcaaucag gacugguuag ccuauauaca agaacugcgg     4140

aacaaaagac ccgcagccac uaagaaagcu ggccaggcaa agaaaaagaa guaaggacua     4200

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     4260

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaag aauu                                                       4334


<210>  3649
<211>  4313
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3649
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcaagcau uuaccaggag      240

uucguuaaca aguauucccu gucuaaaacc cucagguuug aacuuauacc acaaggaaag      300

acacuggaga auaucaaggc ccgggguuug auuuuagacg augaaaaacg cgcgaaggac      360

uacaaaaagg cuaaacagau cauugauaag uaucaucagu ucuuuaucga ggaaauacug      420

ucaagugugu gcaucagcga ggaccuacuc cagaacuacu ccgauguaua uuucaagcug      480

aaaaagucug acgaugacaa ucuucaaaaa gauuuuaagu cggccaaaga cacuauuaag      540

aaacagaucu cagaguacau uaaggauagc gaaaaguuca aaaaccuguu uaaucagaac      600

uugaucgacg caaagaaagg ccaggagagu gaucucauac uguggcugaa gcaauccaaa      660

gacaauggga uugaacucuu caaggccaac ucugacauca cggauauuga cgaggcuuua      720

gagaucauca agagcuuuaa aggauggacc acauauuuca agggcuuuca cgaaaaucgu      780

aaaaacgugu acuccucaaa ugauauuccg acaaguauaa ucuaccgaau ugucgacgau      840

aaccugccca aguuccuuga gaauaaagca aaguaugaga gcuugaagga caaagccccu      900

gaggcuauca acuacgaaca gauaaagaaa gaucuggccg aggagcuaac cuuugacauu      960

gauuauaaga caucugaagu gaaucagaga guuuucuccc ucgacgaggu guucgaaauc     1020

gcgaacuuua acaauuaccu gaaccaguca gguaucacca aauucaauac uauuaucggg     1080

ggaaaguuug ucaacggcga gaauacaaag aggaaaggca uuaacgaaua uauaaaucuu     1140

uacagccaac agaucaacga uaagacccug aaaaaguaua aaaugagugu guuguucaag     1200

cagauucucu cugacacgga guccaagagc uuuguaaucg acaaacugga ggaugacucg     1260

gauguuguga cuaccaugca aucuuucuac gaacagaucg cagccuuuaa gacagucgag     1320

gaaaaaucca uuaaggagac uuuaucacuc cuguucgacg aucuuaaagc ucagaagcug     1380

gacuugagua aaauauacuu uaagaaugau aagagccuaa ccgaccuguc ccagcaagug     1440

uucgaugacu auucugugau cgggacagcc guccucgaau acauuaccca gcagaucgca     1500

cccaaaaacc uggauaaucc aagcaagaaa gagcaggagc uuauugcuaa gaaaacggaa     1560

aaggccaagu aucugucauu ggagacuauc aaacucgcgc uggaagaguu uaacaagcau     1620

cgggacauag auaaacaaug ucgcuucgag gaaaucuuag ccaauuuugc agcuauuccu     1680

augaucuucg acgagauugc ccagaacaag gacaaucugg cucagaucag uauaaaauac     1740

cagaaccaag gaaagaagga ucuacuccag gccuccgcag aagacgaugu uaaagccauu     1800

aaggaccugc uugaucagac aaacaauuug cugcacaaac ucaagaucuu ccauauuagc     1860

cagucugagg acaaagcuaa cauccuggau aaggacgaac acuuuuaucu uguguucgag     1920

gagugcuacu uugaacuggc gaauauagua ccauuguaua acaaaaucag aaauuacauu     1980

acccaaaagc ccuacucaga ugagaaguuc aaacucaacu uugaaaauag uacucuggca     2040

aacgguuggg acaagaauaa agagccugau aacacagcca ucuuauucau uaaggacgau     2100

aaauauuacc ugggcgugau gaauaagaag aacaauaaaa ucuuugacga caaggcuaua     2160

aaagaaaaca agggggaggg auauaaaaag auugucuaca agcuacuccc aggcgccaau     2220

aaaaugcugc cgaagguguu cuuuagcgca aaauccauca aguucuauaa ccccucugag     2280

gauauccuua ggauucggaa ccacucgacc cauacgaaaa augguagccc ucagaagggg     2340

uacgaaaagu uugaguucaa caucgaagac ugucgcaaau uuauugauuu cuauaagcag     2400

uccauaucaa aacacccaga guggaaggac uucggauuuc gauucaguga uacccaaaga     2460

uacaauucua ucgacgaauu uuacagggag guugagaacc agggcuauaa auugacauuc     2520

gaaaauauua gcgaguccua caucgauuca guggucaacc agggcaagcu guaucucuuu     2580

cagauuuaca auaaggacuu cagcgccuau ucuaaagggc ggcccaaccu gcauacuuua     2640

uacuggaagg cucuguuuga ugaacguaau cuucaagacg ugguauauaa auugaacgga     2700

gaggccgaac ucuucuaccg caagcagucc aucccuaaaa agauaaccca cccagcaaaa     2760

gaagcgaucg cuaauaagaa caaggauaau cccaaaaagg agaguguuuu ugaauacgac     2820

cugauuaaag acaagagauu cacagaggau aaauuuuucu uccacugccc gaucacuauu     2880

aacuuuaaga gcucuggugc caauaaguuc aacgacgaaa ucaaccuacu gcucaaagag     2940

aaggcaaaug augugcauau acuguccauu gacaggggcg agcggcaccu ugccuauuac     3000

accuuggucg augggaaagg aaacaucauc aagcaggaca cauuuaauau uauaggcaac     3060

gaucgaauga aaaccaauua ucaugacaag cuggcugcca ucgaaaagga ucgcgacuca     3120

gcaagaaaag auuggaagaa aauuaacaau aucaaggaga ugaaagaagg uuacuuaagu     3180

cagguggucc acgagauugc uaagcucgug aucgaauaua acgccauagu uguguucgag     3240

gaccugaauu uuggguucaa gaggggccgg uuuaaaguag agaagcaagu cuaccagaaa     3300

cuugaaaaga ugcugaucga gaaauugaac uaucucgugu ucaaggauaa ugaauuugac     3360

aaaacuggag gcguucugcg cgcguaccag cuaacggccc cuuucgagac auuuaagaag     3420

auggggaaac agaccggaau uaucuacuau gugcccgcug guuucacuag caagauuugu     3480

ccagucaccg gcuuugugaa ccaacuguac ccuaaauaug aaucgguauc caagucucag     3540

gaguucuuca gcaaauuuga caagaucugc uacaaucucg auaaggggua uuucgaguuu     3600

ucauucgacu acaaaaacuu uggagauaag gcagccaaag gcaaguggac aauagcuagu     3660

uucgguucca gacugauuaa uuuuaggaac ucugacaaaa aucauaacug ggauacccgu     3720

gaaguguauc ccacuaagga gcuugaaaag uugcugaaag acuacagcau cgaguacggg     3780

cacggcgagu guaucaaggc cgcaauuugc ggagaauccg auaaaaaguu cuuugcgaaa     3840

uuaacaucag uucucaacac gauccugcag augcggaaua gcaagaccgg cacugagcuu     3900

gacuaucuga uaaguccagu cgccgaugug aacgggaauu ucuuugacuc ucgacaagcu     3960

cccaagaaca ugccucagga ugccgacgca aauggagcuu accacauugg ucuaaaaggc     4020

cucaugcugu uggggagaau caagaacaau caggaaggca aaaagcugaa cuuagugauu     4080

aaaaaugagg aauauuucga guuuguccag aacaggaaua acaagcgccc agccgcaacu     4140

aaaaaggccg gacaagcuaa gaaaaagaaa uaaggacuag ucccuguucc cagagcccac     4200

uuuuuuuucu uuuuuugaaa uaaaauagcc ugucuuucag aucuaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4313


<210>  3650
<211>  4466
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3650
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcagacaag      180

aaauacagca uuggacugga uauagguaca aacuccguug gcugggccgu gaucacagac      240

gaguauaagg uaccaucuaa gaaauucaag gugcucggga auacugauag gcauucaauu      300

aaaaagaacc uuaucggagc gcuguuguuu gacaguggcg aaacggcuga ggccacccgg      360

uuaaaacgca cagcacgacg uagauacacu aggcggaaga aucgcauuug cuaucugcag      420

gaaaucuuca gcaacgagau ggccaagguc gaugacuccu uuuuccacag acuagaagag      480

ucuuuucucg uggaggaaga uaaaaagcau gagaggcacc cgauauucgg uaauaucguu      540

gacgaagugg cuuaccauga gaaauauccc accauuuacc aucugcggaa gaaacuuguc      600

gauucgacag acaaggcaga ucugcgcuug aucuaucucg cccuggcuca caugauuaaa      660

uuucgagggc acuuccugau cgagggagac cucaacccug auaauucaga cguggacaag      720

uuauuuauac aacugguaca gaccuacaac cagcuuuucg aagagaaucc aauuaacgcc      780

agcggcguug augcgaaggc aaucuugagu gccagacugu ccaaaucuag gcggcuagaa      840

aaucucauug cucagcugcc cggcgagaag aaaaacgggc uuuuuggaaa ucugaucgcc      900

uugagccucg gucugacucc uaacuucaag uccaauuuug acuuagcaga agaugcuaaa      960

cugcaacucu caaaggacac auacgaugac gaucuugaca accuguuggc ccagaucggc     1020

gaucaguaug cggaccuauu ccuggccgca aagaaccuca gugaugcuau ucugcuuagc     1080

gacauacugc gugugaauac cgagaucacg aaagccccau ugucugcuuc caugauuaag     1140

cgcuacgacg agcaccauca ggaucucacu cuguuaaaag cccuggucag acaacagcua     1200

cccgaaaagu auaaagagau cuucuuugac cagucaaaga acggguacgc aggauauaua     1260

gauggcggug ccagccagga agaguucuac aaguuuauua aaccuauccu cgaaaagaug     1320

gacgggaccg aggagcugcu ugugaaauug aauagggaag aucugcuccg gaagcaacga     1380

acauucgaca acggaaguau cccacaccag auucaccugg gcgagcuuca ugcuauccug     1440

cgcagacagg aagauuuuua uccguucuug aaagacaaua gggagaagau ugaaaagaua     1500

cucacuuuuc ggauccccua cuacgugggc ccucuggcgc gcgggaacuc uagauucgca     1560

uggaugacca ggaaauccga ggagacaauu accccaugga auuuugaaga ggucguugau     1620

aagggagcca gcgcucaauc guucaucgaa cguaugacga acuuugacaa aaauuuaccc     1680

aacgagaagg ugcugccuaa acacucucua cucuaugagu acuucacugu auauaaugaa     1740

cugacaaagg ugaaauacgu caccgagggu augcggaagc cagccuuucu uuccggcgaa     1800

cagaagaaag caaucgugga uuugcuguuc aagacuaacc gaaaaguuac agugaagcag     1860

cucaaagagg acuauuucaa gaagauugaa uguuuugauu cagucgagau aaguggggug     1920

gaggacagau ucaaugccag ccugggaacc uaccaugacu uacugaaaau cauuaaggau     1980

aaagacuuuc uugauaacga agagaaugaa gacaucuugg aggauauugu acucacgcug     2040

acccuauucg aagacaggga gaugaucgag gaacgccuga agacauaugc ucaccucuuu     2100

gaugacaaag uuaugaagca gcugaagcgg agaagguaca cuggcugggg ucgccuuucc     2160

cgaaaauuga uaaacgggau ccgggauaag caaucuggca aaaccauucu ggacuucuua     2220

aagagcgaug gauuugccaa ccguaauuuc augcagcuca uccaugacga uucacugaca     2280

uuuaaagagg acauucagaa ggcacaggug aguggccaag gggacucccu ucacgaacac     2340

aucgcgaacc uggcuggaag ccccgccaua aagaaaggua uuuugcagac ugucaaggug     2400

gucgaugagc ucgugaaagu uaugggcaga cauaagccgg aaaauaucgu gauugagaug     2460

gcaagggaga accagaccac acagaaaggg caaaagaacu cucgcgaacg gaugaaaaga     2520

aucgaggaag gaauaaagga gcugggcuca cagauccuaa aggagcaccc uguagaaaau     2580

acccagcugc agaaugagaa acucuaccug uauuaccuuc aaaacgguag ggacauguau     2640

gucgaucagg aauuggacau uaaucgacug agugauuacg acguggauca uaucguuccc     2700

cagagcuucu uaaaggacga uuccauugac aacaaagugc ucacucgcuc ugauaagaau     2760

cgggggaaau cggacaacgu cccaagcgag gaagugguaa agaagaugaa aaauuauugg     2820

agacaacugc uuaacgccaa gcugaucacg cagaggaaau uugacaaucu aacaaaggcu     2880

gagcguggcg gacucuccga gcuggauaaa gccggcuuca uaaagcgcca guugguggaa     2940

acccggcaga uuacuaagca cguugcacaa auccuggacu caagaaugaa caccaaauac     3000

gaugagaacg acaaguuaau cagggaaguc aaagugauua cacucaagag uaaacuggug     3060

ucugauuuuc gaaaggacuu ccaguucuau aagguccggg agaucaauaa cuaccaccau     3120

gcucacgaug ccuaccuuaa ugcgguugug gggaccgccc ugauuaaaaa guauccuaaa     3180

uuggaaagcg aguuugugua cggagacuau aagguauacg auguccgcaa aaugauagcu     3240

aaguccgagc aggaaaucgg uaaggcaacu gccaaauauu ucuuuuacuc aaacauuaug     3300

aauuucuuua agacagagau cacgcucgcu aacggcgaaa uuagaaaaag gccccugauc     3360

gagaccaaug gggaaacugg agagauagug ugggacaagg gccgggauuu cgccacaguu     3420

cgaaaagugc uaagcaugcc acaggucaac aucgugaaga aaaccgaggu ucaaacaggu     3480

ggguuuucua aggaauccau ucugcccaag agaaauagug acaaacuuau cgcacgcaag     3540

aaagauuggg acccuaagaa auauggcgga uucgacagcc caacuguggc guacucugua     3600

cucgucgugg ccaaggugga gaagggcaaa uccaagaaac ugaagucagu caaagaauug     3660

cuggggauua ccaucaugga gaggaguagc uuugagaaga acccgauaga uuucuuagaa     3720

gcuaagggau acaaagaggu uaagaaagac cucauuauca agcugcccaa auauucgcuu     3780

uuugaacugg agaauggccg uaagcggaug uuggccuccg cuggugaacu ccagaagggg     3840

aacgagcugg cacuaccuuc uaaauacgug aauuuccugu aucuugccag ccauuacgag     3900

aagcucaaag gaucaccaga agauaacgag cagaagcagc uguucguaga acaacacaaa     3960

cauuauuugg acgagaucau ugaacagaua agugaguuuu ccaagagagu gaucuuagca     4020

gaugccaauc uggacaaagu ccucucugcu uacaacaagc acagggauaa gcccauucgc     4080

gagcaggcgg aaaacaucau ucaccuguuc acgcuuacca aucugggcgc cccugcagcu     4140

uuuaaauauu ucgacacaac uaucgaucga aagagauaca ccagcacaaa agagguguug     4200

gacgccacuc uaauacauca auccaucacc ggucuguacg aaacgcggau ugaucucuca     4260

cagcugggcg gggacaagag gccagccgcu accaaaaagg caggacaggc caagaaaaag     4320

aaauaaggac uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua     4380

gccugucuuu cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa agaauu                                          4466


<210>  3651
<211>  5249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3651
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaacuuc      180

aagauucugc caauagccau cgaccucgga guuaaaaaua ccgguguguu uagcgcguuc      240

uaccagaagg gcacaucccu ugagaggcug gauaacaaga augggaaagu auaugaauug      300

ucuaaggacu cauacacuuu acugaugaac aaucggacgg cucgccgaca ucaacguaga      360

ggaauugaua ggaaacagcu agugaagcgg cucuuuaaac ugaucuggac cgagcagcuu      420

aaccuggaau gggacaagga uacacagcaa gccauuaguu ucuuguuuaa ucgcagaggc      480

uucagcuuua ucacugacgg uuauuccccg gaguaccuca acauaguccc cgaacaggug      540

aaggcaaucc ugauggauau uuucgacgau uauaaugggg aggacgaucu ggacucuuac      600

cucaaauuag ccaccgagca ggaaucgaag aucucagaga uuuauaacaa acugaugcag      660

aagauccuug aauuuaaauu gaugaagcug ugcacagaua uaaaagacga caagguuagc      720

accaagacuc uaaaagagau uacaaguuac gaguucgaac uccuggcuga uuaccuugca      780

aauuauuccg agucucugaa gacccaaaaa uuuagcuaca cggacaagca gggaaacuug      840

aaagaacucu ccuauuacca ccaugauaag uauaauaucc aggaguuccu gaagaggcac      900

gccacuauua acgaccggau cuuagauacc cugcucacag acgaucuuga caucuggaau      960

uuuaacuucg aaaaauucga uuuugacaag aacgaggaga aacugcagaa ucaagaagau     1020

aaggaccaua uucaggcuca cuugcaccau uucguguuug ccgucaacaa aauaaaguca     1080

gagauggcga guggcggccg ccaccgaagc caguacuucc aagaaaucac uaaugugcua     1140

gacgagaaca aucaucagga aggguaucug aagaacuuuu gugagaaucu ccacaacaaa     1200

aaguacucua aucuguccgu uaaaaaccuu guaaaucuga uuggaaacuu gucaaaucuc     1260

gagcugaagc cuuuaagaaa auacuucaac gauaagaucc acgcaaaggc cgaccauugg     1320

gaugaacaga aauuuaccga gacauauugc cacuggauac ugggugaaug gagggugggc     1380

gucaaggacc aggauaaaaa ggacggggcu aaauacagcu auaaggaucu auguaaugag     1440

cucaaacaaa aggugaccaa ggccggacug guggacuucc uuuuggaacu ggauccaugc     1500

cggacgauuc ccccuuaccu cgacaacaac aaucguaaac cacccaagug ucagagucug     1560

auccuuaacc cuaaauuucu ggauaaucag uauccaaacu ggcagcaaua cuugcaggag     1620

cucaagaaac ugcagucuau ccagaauuau uuagacuccu ucgagacuga ucugaagguc     1680

cuaaagagcu cgaaagacca accguacuuu guugaauaca agucuuccaa ccagcagauu     1740

gcaucaggcc agcgcgacua uaaagaucuc gacgcuagaa uccugcaauu cauuuuugau     1800

agggugaagg ccagugacga gcuuuugcug aaugaaauau acuuccaggc gaaaaagcuc     1860

aagcagaaag ccagcuccga gcuggagaag uuagaaucua gcaaaaagcu ggaugaggua     1920

aucgcaaacu cacaacuuag ucagauuuug aaaucccagc auacaaaugg uaucuucgaa     1980

caggggaccu uucuccaccu ggugugcaag uauuacaagc aacggcagcg agcucgcgac     2040

agcagacuau auaucaugcc cgaguacagg uaugauaaaa agcugcauaa auacaacaau     2100

acuggacggu ucgacgauga caaccagcuc cugacauacu guaaucacaa gccucgccag     2160

aaaagauauc aacuuuugaa cgaucuggcc ggcgucuuac aggugucucc aaauuuucuc     2220

aaggacaaaa uuggcucaga ugacgaccug uucauaagua aguggcuugu ugaacacauc     2280

aggggguuua agaaagcaug cgaggauagc cugaagauuc agaaagacaa ccguggauug     2340

cucaaccaua agaucaauau ugcccggaac accaaaggua agugugagaa ggaaaucuuc     2400

aaucugauau gcaaaaucga gggcuccgaa gauaagaaag ggaacuacaa gcacggacua     2460

gcauaugagc ugggcgugcu ccuguuuggu gaacccaaug aggccucuaa accugaguuc     2520

gaccgaaaga uuaagaaauu uaacucgauc uacagcuucg cucagauuca acagaucgcg     2580

uuugcagaaa gaaaggggaa ugccaacacg ugugcugucu gcuccgccga uaaugcacau     2640

aggaugcagc aaauaaaaau uaccgagcca guggaagaca acaaggauaa aaucauucuu     2700

ucagccaagg cucagcgcuu gcccgccauc ccgacacgga uaguagacgg cgcaguuaag     2760

aaaauggcga cuauccuggc uaagaauauu guggaugaca acuggcagaa uaucaaacag     2820

gucuuaagug ccaagcacca acuccacauu ccuaucauaa ccgagucuaa cgcauucgaa     2880

uuugagcccg cccuggcuga ugugaaagga aagagccuua aggacagaag gaaaaaggcc     2940

cuggagcgca uuuccccaga aaauaucuuc aaagacaaga acaaccgaau caaagaguuc     3000

gcaaagggca uuucagcuua uagcggggcc aaucuaacag auggagacuu ugauggugcg     3060

aaagaagaac ucgaccauau cauuccucgg ucucacaaga aguacggcac ucugaacgau     3120

gaggccaauu ugauaugcgu cacccguggg gacaacaaaa auaagggaaa cagaaucuuc     3180

ugucugaggg auuuagcuga caauuauaaa cucaagcagu uugaaacaac cgaugaccug     3240

gagauugaga aaaagaucgc agauacuauu ugggacgcca acaagaaaga uuucaaguuu     3300

ggcaauuacc gcuccuucau caaccuuacg ccccaggagc agaaggcuuu ucggcaugcc     3360

cuguucuugg cagacgaaaa uccaauaaaa caagcgguga ucagagccau uaacaauagg     3420

aaccgaacau uuguuaaugg uacccagcgc uacuucgcug aggugcucgc caacaauauc     3480

uaucugcggg cuaaaaagga gaaccuaaac acugacaaga uuaguuuuga uuacuucggg     3540

auccccacca uaggcaaugg aagaggcauu gcugaaauca ggcagcugua ugagaaagua     3600

gacagcgaua uccaggccua cgcaaagggg gacaaaccuc aagccucuua uucccaccuu     3660

auugaugcua ugcucgcguu cugcauagcc gcagacgaac aucguaacga uggaucaauc     3720

ggucuggaga uugacaagaa uuacaguuug uauccacugg auaaaaacac aggcgaaguc     3780

uuuaccaagg acaucuucag ccagauuaag aucacugaua augaguuuuc ggacaaaaag     3840

uuagugcgca aaaaggcuau agagggguuc aacacacacc ggcagaugac gagagaugga     3900

aucuacgccg aaaauuaccu cccgauucug auccacaaag agcuuaacga aguuaggaag     3960

ggcuauaccu ggaaaaauuc cgaggaaauu aagaucuuua aggguaaaaa guacgacaua     4020

cagcaacuga acaauuuggu guauugucuc aaauucgucg acaagcccau uucuaucgau     4080

auccagauua gcacucugga ggagcuacga aacauccuga caaccaauaa cauagccgcu     4140

acagcagaau acuauuacau uaaucuuaaa acucagaagc uccaugagua uuacaucgaa     4200

aacuacaaca ccgcccuggg guauaagaaa uacucaaagg agauggaguu uuugcggagu     4260

uuagcguauc gcuccgaaag agugaaaauu aagucuaucg acgauguaaa acaagugcug     4320

gacaaggaua gcaauuucau aauuggcaag aucacgcucc cuuuuaaaaa ggaguggcag     4380

aggcuguacc gggaauggca gaacaccaca aucaaagacg auuaugaguu ccuuaagucc     4440

uuuuucaaug uuaaaucaau uacuaagcug cacaagaaag uccgaaagga cuuuagcuug     4500

ccaaucagua ccaacgaagg aaaauuccua gugaagagaa aaacauggga uaauaacuuc     4560

auuuaccaga uacugaauga cucugauucc cgcgcugacg gcacuaagcc cuuuaucccu     4620

gccuucgaca uuagcaaaaa cgagaucgug gaggcaauca uugauucguu uaccucaaag     4680

aauauauucu ggcucccaaa gaacaucgaa cugcaaaaag ucgacaauaa gaacauuuuu     4740

gccaucgaua cgucuaaaug guucgagguu gaaacaccca gugaccuuag ggauauuggg     4800

aucgcuacca uacaguauaa gaucgacaau aacucccguc cgaaagugcg ggugaaguua     4860

gauuacguaa uugacgauga cagcaagauc aauuacuuua ugaaccauuc ucugcucaaa     4920

agcagauauc cugauaaggu cuuggagauu cugaaacagu ccacuaucau agaguucgaa     4980

ucaaguggau uuaacaagac aauuaaagag augcucggca ugaagcuggc agguaucuac     5040

aaugaaacca gcaacaauaa gaggccagcc gcgacuaaaa aggcugggca ggccaaaaag     5100

aaaaaguaag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     5160

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5220

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       5249


<210>  3652
<211>  3521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3652
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaagagg      180

aacuacauuc ugggacucga cauagguauc acaucaguug gcuaugggau uaucgauuac      240

gagacacggg acgugauuga ugccggagua cgccuuuuca aagaagcgaa uguggagaac      300

aaugaaggcc gacguuccaa gagaggugcu aggcggcuga agcgcagaag gcggcaucgc      360

auccagcgag ucaaaaaguu guuauuugac uauaaccugc uaacugauca cucugagcuc      420

ucagggauaa auccauacga agccagagug aaaggacuga gucaaaaacu uagcgaggag      480

gaguucuccg cagcccuguu gcaucucgcu aaaaggcggg gcguucacaa cgugaaugaa      540

gucgaagagg acacgggcaa cgagcugucu accaaggaac agaucucgcg uaauucaaag      600

gcacuggagg aaaaauaugu ggccgagcuc caguuagaac gccugaagaa agauggggag      660

guaagaggaa gcauuaacag guuuaagaca agugacuacg uuaaagaggc uaagcagcuu      720

uugaaagugc aaaaggccua ucaucagcug gaucaguccu ucaucgacac uuacauugau      780

cuacucgaaa cccggcgaac auacuaugag gguccgggcg aagggucucc cuuuggaugg      840

aaggacauca aagaguggua cgaaaugcug augggccacu gcaccuauuu cccugaggag      900

cuucgcagcg ucaaguacgc guauaaugca gaucuguaca acgccuugaa ugaccucaac      960

aaucugguga uaacuagaga cgaaaacgag aaauuagaau auuacgagaa guuucagauu     1020

aucgaaaacg uguucaaaca aaagaagaaa ccaacacuga agcagauugc uaaagagauc     1080

cucgucaaug aggaagauau caaggguuac aggguuaccu ccacggggaa acccgaguuu     1140

acuaaccuua agguguauca cgacauuaag gauauaaccg cccggaaaga aaucauugag     1200

aaugcagagc uguuggacca gaucgcuaag auacuaacaa uuuaccaguc aagugaagau     1260

auccaagagg aacugacuaa ccucaauagc gagcugaccc aggaagagau cgagcagauu     1320

ucuaaccuua aaggauauac aggcacccau aaucuguccu ugaaggccau caaccucauu     1380

cuggacgaau uauggcacac gaaugauaac caaauagcga ucuucaaucg ccugaaacuu     1440

guaccuaaga agguggaccu cucacagcag aaagagauuc caacuacacu ggucgaugac     1500

uuuauccuua gccccguggu uaagagaagu uucauccagu cuauuaaagu gauaaacgcc     1560

aucauuaaga aauacggcuu gccuaaugau aucauuaucg aacuggcaag ggagaagaac     1620

uccaaagacg cucaaaagau gauaaaugaa augcagaagc guaaccggca gaccaacgag     1680

cgaaucgagg aaauuaucag aacuacaggg aaagagaaug ccaaguaucu cauugaaaag     1740

aucaagcugc augacaugca ggagggaaaa ugucuuuaca gccuggaagc uauaccauug     1800

gaggaucucc ugaacaaucc guuuaacuau gaggucgacc acauuauccc caggucggug     1860

ucuuucgaua auuccuucaa caauaaggua uuaguuaagc aagaagagaa cucaaagaag     1920

gguaaucgca ccccuuuuca guaccugagu agcuccgacu cuaaaauuag cuacgaaacg     1980

uucaagaaac acauccuaaa ccucgccaag ggcaaggggc ggauaucaaa aaccaagaaa     2040

gaguaucugc uugaggaaag agauaucaau agguuuagug ugcagaagga cuucauuaac     2100

cgcaauuugg ucgauacacg auacgcaacu cggggacuga ugaaccuccu gcguuccuau     2160

uuuagaguga auaacuuaga cgucaaagug aaaagcauca acggcgguuu caccucuuuu     2220

cugaggcgca aguggaaauu caagaaagag cggaauaagg gguacaaaca ucacgccgaa     2280

gaugcucuua uuaucgcgaa cgcagacuuu auauucaagg aguggaaaaa guuggauaag     2340

gccaaaaagg uuauggaaaa ucagauguuu gaggagaaac aagcugaauc aaugccagag     2400

auugaaacag agcaggaaua uaaggagauc uucaucacuc cccaucagau uaaacacauc     2460

aaggacuuua aggauuacaa auauagucau agaguggaca agaaaccuaa cagggagcuc     2520

auuaaugaca cccuguacag cacacgaaag gaugacaaag gcaacacccu aauaguaaau     2580

aaccugaaug gacucuacga uaaggacaac gauaagcuga aaaagcuaau caauaaaucc     2640

ccagaaaagu ugcugaugua ucaccacgac ccccagacuu accaaaaauu aaagcucauu     2700

auggagcagu auggcgauga aaagaacccg cuguacaaau auuacgagga aacggggaau     2760

uaucuuacaa aguacucuaa aaaggacaac ggaccuguca ucaaaaagau uaaguacuau     2820

gguaauaaac ugaacgccca uuuggauauc accgacgauu accccaacuc gcgcaauaag     2880

gugguuaaac ucagccugaa gccauaucgg uucgacgugu accuagauaa cggcgucuau     2940

aaauucguga cuguaaagaa ucuggacgug aucaaaaagg agaacuacua ugagguuaau     3000

uccaagugcu acgaagaggc caaaaagcuc aaaaagauau caaaccaggc agaauuuauu     3060

gcuaguuucu acaauaacga ccugaucaaa auuaaugggg agcuuuauag agucaucgga     3120

gugaacaaug auuugcugaa caggauagag gugaauauga uugacaucac cuaccgugaa     3180

uauuuagaga acaugaauga uaagcgcccu ccccggauca uuaagacaau agccucuaaa     3240

acccaaagca ucaagaaaua cuccacugac auucucggca accuguauga agucaagagc     3300

aaaaagcacc cacagaucau uaagaaaggu aagagacccg cagcgaccaa aaaggcuggg     3360

caggccaaaa agaagaaaua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3420

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3521


<210>  3653
<211>  4589
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3653
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcacuguuc      180

aacaagugca uuauaaucag cauuaaucuc gacuuuucca acaaagagaa guguaugacc      240

aagccauacu cuaucggacu ugauauuggu acaaauucag uuggcugggc cgugaucacu      300

gacaacuaua aaguaccgag uaagaaaaug aaggugcugg ggaauacgag caaaaaguac      360

auaaagaaaa acuuguuagg aguccugcua uucgauuccg gcaucaccgc ggaagguagg      420

cggcucaagc gcacagcucg acguagauau acuaggcggc gcaauaggau ucuguaccuu      480

caggagaucu uuucuaccga aauggccaca cuggacgaug cauucuuuca aagauuggac      540

gauucguucc ucgugcccga cgauaaacgg gacucaaagu auccuauuuu ugggaaccug      600

guugaggaaa aaguguacca ugaugaguuc ccaaccaucu aucaucugcg caaguaccuc      660

gccgacagca cuaaaaaggc ugauuuacga cuggucuacc uugcauuggc ccacaugaua      720

aaguauagag gacacuuucu gauugagggc gaguucaaua guaaaaacaa ugacauccag      780

aagaacuuuc aggacuuccu agauacauac aaugcuauuu uugaguccga ccucucucug      840

gaaaacagca aacagcuuga ggagaucgug aaggauaaaa ucuccaagcu ggaaaagaaa      900

gacaggauuu ugaaacucuu ccccggcgag aaaaauucag ggauauucag ugaauuucug      960

aaguuaaucg uaggaaacca agccgauuuc cggaaaugcu uuaaccugga cgagaaggcg     1020

agccuccauu ucucuaagga auccuaugau gaggaccuug agacccuguu ggguuacauu     1080

ggcgaugacu auucagaugu uuuucuaaaa gcaaagaaac uguacgacgc cauccuccug     1140

agcggguucc uuacggugac ugacaaugaa accgaggcuc cucugaguuc ugccaugaua     1200

aagcguuaua acgaacacaa agaggauuug gcacuccuga aggaauacau ucgcaauauc     1260

uccuuaaaga cauacaacga ggucuuuaaa gacgauacua agaauggaua ugcuggcuac     1320

aucgacggua aaaccaacca ggaggauuuc uauguguacc ugaagaaucu acucgccgaa     1380

uuugaggggg cggacuauuu ccuggaaaag auugauagag aggacuuucu uaggaagcag     1440

cggacauucg auaacggaag caucccauac cagauucacu ugcaagaaau gcgagccaua     1500

cuggacaaac aggcaaaguu uuaucccuuc cucgcuaaga auaaagagcg caucgagaag     1560

auucugaccu ucagaauccc uuacuacgug ggcccacuug ccaggggcaa cucggauuuu     1620

gcuuggucua uccggaaacg caaugaaaag auuacgccgu ggaacuucga ggacgucaua     1680

gauaaagaau ccucagccga ggcauuuauc aauagaauga caaguuucga ccuguauuug     1740

cccgaggaaa agguucuccc uaagcauagc cuguuauacg agacuuuuaa cguguauaau     1800

gaacugacca aaguaagguu cauugccgag uccaugcgug acuaccaguu ucuagauucu     1860

aagcagaaaa aggacaucgu gcggcucuau uucaaagaua agcgaaaggu cacugacaaa     1920

gauauuaucg aauaccugca cgcuauauau ggguacgacg gaaucgagcu uaaggguauu     1980

gagaaacaau uuaacagcuc auugaguaca uaccaugauc ugcucaacau cauuaaugac     2040

aaggaguucc uggaugacuc cagcaacgaa gcgaucauag aagagauuau ccacaccuua     2100

acgauuuuug aagauagaga gaugaucaaa cagaggcugu cuaaguucga gaauauauuu     2160

gacaagucag ugcuuaaaaa guugagucgc cggcacuaua ccggcugggg gaaacucagc     2220

gcaaagcuga ucaacggaau uagagaugaa aaauccggca auacaauccu agacuaccug     2280

auugacgaug guaucucuaa caggaauuuc augcagcuca uacaugacga ugcccugucg     2340

uucaagaaaa agauucaaaa ggcucagauc aucggggacg aggauaaagg caacauuaag     2400

gaaguuguga aaagccuucc aggauccccc gccaucaaga aaggcauuuu gcagucaaua     2460

aagaucgucg acgagcuggu gaagguaaug ggagggcgca aaccugaaag uauuguugug     2520

gagauggcac gagagaauca guauacuaac caagguaagu cuaauagcca gcagcgguua     2580

aaacgucucg aaaagucccu gaaagagcuu ggcucaaaga uccugaagga aaacauucca     2640

gccaaauuga gcaagaucga uaauaacgcu cuccagaaug acagacugua ccuauauuac     2700

cugcaaaacg ggaaagauau guauaccgga gacgaucucg acauagacag gcugucuaac     2760

uacgauaucg accacauuau cccccaggcc uuucuuaagg auaauuccau ugacaacaaa     2820

gucuugguga guagcgcauc uaaucgcggc aaguccgaug acuucccguc acuggagguc     2880

gugaagaaac ggaagacauu uugguaccag uuacucaaaa guaagcugau cagccagaga     2940

aaauucgaua accuuacuaa ggcggagagg ggugggcugc uaccugaaga caaggcuggc     3000

uuuauacaac gacagcucgu ugagacccgc cagauuacaa aacauguggc ccggcuguug     3060

gaugaaaagu ucaauaacaa aaaggacgag aauaacagag caguaaggac cgucaaaauc     3120

aucacucuga agucgacguu agugucccag uuucguaaag auuucgaacu cuauaagguu     3180

cgcgagauua augacuuuca ccaugcccac gaugcuuacc ugaacgccgu gauagcaucu     3240

gcccuucuga agaaauaucc caaguuggag ccagaguucg ucuacggaga cuauccuaaa     3300

uacaauagcu uucgggaaag aaagucagcu acagaaaaag uguauuucua caguaacauc     3360

augaauauuu ucaagaaguc caucucucuc gcggacggca ggguaauuga gcgaccccug     3420

aucgaaguga acgaggagac cggggaaagc guuuggaaua aagaguccga ucuagccacu     3480

guccggcgcg ugcugucaua cccacaagug aacgucguua agaaagugga agaacagaac     3540

cacggacuug acagagguaa gcccaaaggc cucuuuaaug cuaaccugag caguaagccu     3600

aagccaaauu cuaacgaaaa uuuggugggg gcaaaagagu aucuggaucc gaagaaauac     3660

ggaggcuaug ccgguauauc caacagcuuc gcuguauuag ucaaggggac caucgagaaa     3720

ggcgccaaga agaaaauuac aaaugugcuc gaauuucagg gaaucucgau ucuggacagg     3780

aucaacuacc ggaaggauaa acuuaauuuc cuguuggaga agggcuauaa agacauagaa     3840

cucauuaucg agcugcccaa guacucacua uuugagcugu cugaugggag ucgaagaaug     3900

cuugcaucca uccucagcac caacaauaaa cgcggagaaa uucauaaggg caaccaaauc     3960

uuccugucuc agaaguuugu uaaauuguua uaucacgcga agaggauaag caauacuauu     4020

aacgagaauc aucguaaaua cguggaaaac cacaagaaag aguucgagga acuguuuuac     4080

uauauccucg aguucaauga aaacuacguc ggugccaaga agaacgggaa acugcuuaau     4140

uccgcuuuuc agucauggca gaaccacagu auugacgagc uguguagcuc uuucaucgga     4200

ccuacaggcu ccgaacggaa ggguuuguuu gagcuaacgu caagaggcag ugccgcagau     4260

uucgaguucc ugggggugaa aauaccaagg uaucgcgacu acacccccag cucccuccug     4320

aaggaugcua cucuuauuca ucaaucuguu acaggauuau augaaacccg aaucgaccug     4380

gccaaacucg gcgaggggaa gagaccugca gccacaaaga aagcuggaca ggcgaagaaa     4440

aagaaauaag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     4500

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       4589


<210>  3654
<211>  3665
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3654
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaugguac      180

gccagccuga uguccgcgca ucaccucagg guuggaauug acguggguac ccauucugua      240

ggccuugcua cacugcgggu ggaugaccac gggacuccaa uagaguuguu aucagcccug      300

agucauaucc acgauagcgg agucggcaag gaagguaaaa aggaccacga uacgcgcaag      360

aaacuauccg ggauugcacg acgugccaga aggcuccugc aucaccggcg cacccagcuu      420

caacagcugg acgagguguu gagagaucuc ggauucccga uccccacacc uggcgaauuu      480

cuggaccuga acgagcagac ugauccauau aggguuuggc gggugcgcgc ucgacucguc      540

gaagagaagu uacccgagga acugagaggc ccugcaauuu cuauggccgu gaggcauauc      600

gcucggcacc guggguggcg caauccauac ucgaaaguag agucacuuuu gagccccgcc      660

gaagagaguc cuuucaugaa ggcgcugaga gagaggauac uagcaaccac aggagaaguu      720

cucgacgaug guaucacccc aggccaggcc auggcucaag uggcccugac ucacaacauu      780

uccaugcggg ggccggaggg aauccuuggc aaacugcauc agucugacaa ugcaaacgaa      840

auucgaaaga ucugcgcucg ccaggguguc agccccgaug uguguaagca guugcucaga      900

gccguguuua aagcggacuc cccuaggggg ucagccguca gucggguugc accagacccc      960

cugccuggac aaggcagcuu ccgcagagcu ccaaagugcg aucccgaguu ucagagguuc     1020

cguauaauuu cuaucguggc caauuuacgg auuuccgaaa caaaaggcga gaaccgaccg     1080

cugaccgcug acgagagaag gcacguagug acguuucuca cugaagauuc acaggccgac     1140

cuuaccuggg ucgauguggc agagaagcug gggguucauc gccgggacuu gagaggaaca     1200

gccgugcaca cugaugacgg ugaaaggagc gcugcgcgcc cucccaucga ugcaaccgac     1260

cgaaucaugc ggcagacaaa aauuaguucu cuaaagaccu ggugggagga agccgauucc     1320

gagcaacgug gcgcuaugau aagauaucug uacgaggacc caacggacag cgaaugugcc     1380

gagaucauug cagaacuccc ugaggaagau caggccaaac uggacucgcu ucaucugccc     1440

gcugggaggg ccgcauauuc ucgcgagucc uugacugcgc ucucagauca caugcuggcu     1500

acaaccgacg auuuacacga ggcccggaag agacuguucg gagucgacga uaguugggca     1560

ccacccgccg aagcuaucaa ugccccugug ggcaacccaa gcguagacag gacucuaaag     1620

auaguugguc gauaccucuc cgcaguggag ucuauguggg ggacaccgga agucauucau     1680

guggagcacg uccgcgaugg cuuuaccagc gagcggaugg cugacgaaag agauaaagcc     1740

aauaggcguc gcuauaacga caaucaggag gcgaugaaga aaauccaacg ggauuacgga     1800

aaggaaggcu acaucucaag aggggacauu gugaggcugg acgcccuuga guugcaggga     1860

ugcgcuuguc uguauugcgg uacgaccauc ggcuaccaua caugucagcu cgaucacauu     1920

guuccccagg cagggccugg aaguaacaau cgacggggca accugguggc cguaugcgaa     1980

cgcuguaaua gauccaaaag caacacucca uucgcugucu gggcccaaaa gugcgguaua     2040

ccccacgugg ggguuaagga ggcaaucggc agggugcggg gauggcgaaa acagaccccu     2100

aacacaucuu cagaggaccu uacuagacug aagaaagaag ucauugcgcg cuugaggcgu     2160

acccaggagg auccagaaau cgacgagcgg aguauggaaa gcguggccug gauggcuaau     2220

gagcuccauc acagaaucgc cgcagcuuau cccgagacaa ccguaauggu guacaggggc     2280

uccauuacug ccgcagcccg caaggcugcg gggauagauu cucgaaucaa ccugauugga     2340

gaaaaaggua gaaaggaccg gaucgauagg cgccaucacg ccguugacgc aucggucgug     2400

gcuuuaaugg aggccagcgu ggccaagacg cuggcugaac guuccucacu aagaggcgag     2460

cagcggcuca cagggaaaga acaaaccugg aagcaguaua cuggaaguac cgucggcgca     2520

agggagcauu uugagaugug gcgcggucac augcugcacc uuacagaauu guucaaugag     2580

cgacuggccg aagauaaagu uuacgugacc cagaacauua gacuccggcu gucugacggg     2640

aaugcgcaua cugugaaccc gagcaaguua guaucccaca ggcugggcga uggacuuaca     2700

guccagcaaa ucgaccgcgc uuguacgccu gccuuguggu gcgcacucac cagagagaaa     2760

gauuuugacg agaagaaugg ccugccagcc agggaagacc gggcuauacg ugugcauggg     2820

cacgagauca agucaagcga uuauauucag guuuucucua aacgcaagaa aacugacucc     2880

gaucgagacg aaacacccuu cggagcaauc gccgugagag gcgguuuugu cgagauuggg     2940

ccuaguaucc aucacgcgag gauauaccgg guggaaggaa agaaacccgu uuacgcuaug     3000

cuacgcgugu ucacccacga ucugcucagc cagagacaug gcgaccuguu uucugccgua     3060

auuccaccuc aguccaucuc aaugaggugc gcagagccca aacuucggaa agccauuaca     3120

acugguaacg cuaccuauuu gggcuggguc gugguggggg augagcugga aaucaauguc     3180

gacaguuuca cgaaguacgc cauaggacga uuuuuagagg auuucccaaa caccacaaga     3240

uggcgcaucu guggcuauga cacuaauagc aagcucaccc ugaaaccgau uguucuugca     3300

gcugaagggc uggagaaccc uucguccgcc gugaaugaaa ucguagaguu gaagggaugg     3360

aggguggcga uuaacguccu cacaaaagug caccccacug uugugcggcg ugaugcccug     3420

ggcagaccaa gguacucuag ccgcucaaau cuacccacca guuggacgau cgagaagcgg     3480

ccugcagcua caaagaaggc cggucaagcu aagaaaaaga aauaaggacu agucccuguu     3540

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

gaauu                                                                 3665


<210>  3655
<211>  4526
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3655
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaccaag      180

ccauacagca uuggacugga cauagguaca aacuccguug gcugggccgu gacuacggau      240

aauuauaaag uaccgucuaa gaagaugaaa gugcucggga acaccucaaa gaaauacauc      300

aagaaaaauc uucugggagu cuuguuauuc gacaguggca uuacagcgga ggguaggcgg      360

cugaagcgca cugcucgacg uagauauacc aggcggcgca acagaauccu auaccuccag      420

gaaauuuuua gcacagagau ggccacccug gaugacgcau ucuuucaaag gcuugaugac      480

uccuuccugg ugcccgauga caagcgggau ucuaaauauc cuaucuuugg gaauuugguu      540

gaagagaagg ccuaccauga cgaguuccca acuauauauc accuccgcaa auaccuggcu      600

gauucgacaa agaaagcaga ccugcgacuc guguacuuag cccuggcuca uaugaucaag      660

uauagaggac acuuucuuau ugagggcgag uucaacucaa agaauaacga uauccagaag      720

aauuuucagg acuucuugga caccuacaac gccauuuuug aaagcgaucu gagucuagaa      780

aauuccaagc agcucgaaga gaucgucaaa gacaagauau cuaaacugga gaagaaagau      840

aggauucuua agcuguuccc cggcgaaaag aacagcggga ucuuuuccga guucuugaaa      900

cucauugugg gaaaucaagc ggacuuccgg aagugcuuua accuggauga aaaagcauca      960

uuacauuuca guaaggagag cuaugacgaa gaucuggaga cgcuccuugg uuacaucggc     1020

gacgauuauu cugacguauu ucugaaagcc aagaaguugu acgaugcuau ccuacugucc     1080

ggguuccuca cuguuaccga caaugagaca gaagccccuc ugucaagcgc aaugauuaaa     1140

cguuauaacg agcacaagga agaccuugcu cuguugaaag aguacauacg caacaucagu     1200

cucaagacuu acaaugaagu guuuaaagau gacaccaaga acggauaugc cggcuacauu     1260

gaugguaaga caaaucagga ggacuucuau gucuaccuga aaaaguuacu ggcggaguuu     1320

gaaggggccg auuauuuccu agagaaaauc gacagagaag auuuucucag gaagcagcgg     1380

accuucgaca acggaucuau accauaccag auucaccuuc aagagaugcg agcaauccuu     1440

gauaaacagg cuaaguuuua ucccuucuug gccaaaaaua aggaacgcau cgagaagauu     1500

cugacguuua gaaucccuua cuacgugggc ccacucgcua ggggcaacuc cgacuucgcc     1560

uggagcauuc ggaaacgcaa ugagaagaua acuccgugga acuucgaaga ugugaucgac     1620

aaagagucgu cugcagaagc cuuuauuaau agaaugacau ccuucgaucu guaucuuccc     1680

gaggagaagg uccugccuaa acauucauug cucuacgaaa ccuuuaacgu uuauaaugag     1740

cugacuaagg ugagguucau cgcugaaagu augcgugacu accaguuuuu agacagcaag     1800

cagaaaaagg auaucguacg gcuguauuuc aaagacaagc gaaaagugac agauaaggac     1860

auuauagagu accuacacgc gaucuauggg uacgauggaa uugaacucaa ggguaucgag     1920

aaacaauuua acuccucucu gagcaccuac caugaccuuu ugaauauuau caacgauaag     1980

gaguuccugg acgauucaag uaaugaagca auaaucgagg aaauuaucca cacgcucacc     2040

auuuuugagg acagagaaau gaucaaacag aggcugucca aguucgagaa cauauuugau     2100

aaaagcgucu uaaagaagcu gucucgccgg cacuauacag gcugggggaa acuuucagcc     2160

aaguugauua acggaaucag agacgagaaa aguggcaaua cuauucucga uuaccugauc     2220

gacgacggua uaagcaacag gaauuucaug cagcuaaucc augaugacgc ucuguccuuu     2280

aagaaaaaga uucaaaaagc ccagaucauu ggggaugaag acaagggcaa caucaaggag     2340

gugguuaaau cucucccagg aucgcccgca auaaagaaag gcauucugca gagcaucaag     2400

aucguggaug aacuugucaa agugauggga gggcgcaagc cugaguccau uguaguugaa     2460

auggcccgag agaaucagua uaccaaccaa gguaagucaa auagucagca gcgguugaaa     2520

cgucuggaga agucuuuaaa agaacucggc agcaagaucc ugaaagagaa cauuccagcu     2580

aagcugucca agauagacaa uaacgcccug cagaaugaua gauuguaccu cuauuaccug     2640

caaaacggga aagacaugua uacaggagau gaccuagaua ucgacaggcu gucaaauuac     2700

gacauugauc acaucauucc ccaggcauuc cucaaggaca acagcaucga uaauaaagug     2760

cuggucucuu ccgcgaguaa ccgcggcaag agcgacgaug ugccgucucu ugaagucgug     2820

aaaaagcgga agacuuucug guaccaguug cugaaaucca aguuaauauc acagagaaaa     2880

uuugacaacc ucaccaaggc ugagaggggu gggcugaguc cugaggauaa agccggcuuc     2940

auccaacgac agcuuguuga aacacgccag auuaccaagc auguggcacg gcugcuagac     3000

gagaaguuua auaacaaaaa ggaugaaaau aacagagccg uaaggacugu caaaaucauu     3060

acgcucaaga gcacacuggu gucgcaguuc cguaaagacu uugaguugua uaagguucgc     3120

gaaaucaaug auuuccacca ugcucacgac gccuaccuga acgcaguggu cgcuuccgcc     3180

uuacucaaaa aguaucccaa gcuggagcca gaguuugugu acggagauua uccuaaauac     3240

aauucuuucc gggaaagaaa gagcgcgacc gagaaaguau auuuuuacuc aaacauaaug     3300

aauauuuuca agaaaaguau cucccuugcc gacggcaggg ugaucgaacg accccugauu     3360

gagguuaacg aagagacugg ggagucuguc uggaauaagg aaagcgacuu ggcuaccgug     3420

cggcgcgugc ucuccuaccc acaagucaac guugugaaga aaguggagga acagaaucac     3480

ggacuggaua gagguaagcc caaaggccua uuuaacgcaa aucugucaag caagccuaaa     3540

ccaaacagua acgagaaucu uguaggggcc aaggaauauc ucgacccgaa gaaauacgga     3600

ggcuaugcug guauaucuaa cuccuucaca guccugguga aggggaccau cgagaaaggc     3660

gccaagaaaa agauuacuaa uguuuuggag uuccagggaa ucagcauucu ggauaggauc     3720

aacuaccgga aggacaaauu aaauuuucuc cuggaaaagg gcuauaaaga uauagagcuu     3780

aucauugaac ugcccaagua cucguuguuc gagcucucag acgggucucg aagaaugcug     3840

gcaaguaucc uauccacaaa caauaaacgc ggagagauuc auaagggcaa ccaaaucuuu     3900

cugagccaga aauucgugaa acuucucuau cacgcgaaga ggauaucuaa uacgauuaac     3960

gaaaaucauc guaaauacgu cgagaaccac aagaaagaau uugaggaacu guucuacuau     4020

aucuuggagu uuaaugagaa cuacgugggu gccaagaaaa augggaaguu acugaacagc     4080

gcuuuccagu ccuggcagaa ucacucaauc gaugaacucu guaguagcuu uauuggaccu     4140

accggcucug agcggaaggg ucuguucgaa cuuacuucca gaggcucagc cgcagacuuu     4200

gaguuccugg ggguuaaaau cccaagguau cgcgauuaca cacccaguag cuugcuaaag     4260

gacgcuaccc ugauacauca auccgugaca ggacucuaug aaacucgaau ugaucuggcc     4320

aaacuuggcg aggggaagag accugcagcc accaaaaagg cuggacaggc gaagaaaaag     4380

aaauaaggac uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua     4440

gccugucuuu cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa agaauu                                          4526


<210>  3656
<211>  3608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3656
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcagccgcg      180

uucaagccaa acagcauuaa uuacauacug ggacucgaca ucgguauugc uuccguuggc      240

ugggccaugg uggagaucga ugaagaggaa aacccgauua ggcuuaucga ccugggggua      300

cggguguuug agcgcgcaga aguccccaaa accggagauu cuuuggccau ggcucgacgu      360

uuagcaagau cagugaggcg gcugacacgc agaagggccc aucggcuacu ccgcacucga      420

agacugcuua agagggaggg cguucugcag gcugccaauu ucgacgagaa cgguuugaua      480

aagagucucc cuaauacgcc auggcaacug cgggcggcag cccuggaucg uaaacucacc      540

cccuuagaau ggagcgcugu gcugcuucac uugaucaagc aucgcgggua ucugucccag      600

aggaaaaacg agggagaaac agccgacaag gagcuaggcg cacuccugaa aggcgucgcu      660

gggaaugccc acgcgcuuca gacuggagau uuuaggaccc cugccgagcu ggcauugaac      720

aaguucgaaa aggagucugg ucauauucgg aaucagcgau cggacuacuc acacacauuu      780

agccgcaaag aucuccaagc ugaacugauc uuacuguucg agaagcagaa agaauuuggc      840

aacccacacg ugaguggggg acucaaggag ggcauugaga cccuucugau gacucagaga      900

cccgccuugu ccggugacgc uguacagaaa augcuagggc auugcacauu cgaaccugcc      960

gagccaaagg cagccaaaaa uaccuauacg gcugaaaggu uuaucuggcu gacuaagcuc     1020

aacaaucugc ggauacuuga gcaaggaucu gaacgcccgc ugaccgauac agagagagcg     1080

acuuugaugg acgagcccua cagaaagagc aaacucaccu augcacaggc ccguaagcug     1140

uuaggccugg aagauacagc uuucuuuaaa ggccuacggu acgggaagga caacgccgag     1200

gcauccaccc ucauggaaau gaaagccuau cacgcuauuu cacgagcccu ggagaaggaa     1260

ggacuuaagg acaaaaagag uccuuugaau cugagcccag agcuccagga ugagaucggu     1320

acggcauucu cucuguuuaa aacugacgaa gauauuacag gcagacuuaa ggacaggauc     1380

cagcccgaga uccuggaagc guugcucaaa cauauuuccu ucgauaaguu cguucaaaua     1440

ucacugaagg cuuuacgccg gaucgugccu cugauggagc aggggaaaag auacgacgag     1500

gccugugcag aaauuuacgg agaucacuau ggcaagaaaa acaccgagga aaagaucuac     1560

cuaccaccca uaccggccga cgagauuagg aacccugucg ugcuccgcgc ucugagccag     1620

gcccgaaaag ugaucaaugg ugucguucgg cguuauggga gucccgcaag aauccacauu     1680

gaaacugcua gggagguggg caagucuuuu aaggaucgca aagagaucga aaagcggcaa     1740

gaggaaaaca gaaaagacag ggagaaggcc gcggccaaau uccgagaaua cuuuccaaau     1800

uucguaggag agccuaaguc caaagauauu cuuaaguugc gccuguauga gcagcagcau     1860

ggcaagugcc ucuacagcgg gaaagaaaua aaccugggac gguuaaauga gaaggguuau     1920

guggaaaucg accacgcucu gcccuuuucg agaacauggg acgauucuuu caacaauaaa     1980

guccuugugu ugggcuccga gaaccagaau aaggggaacc aaaccccaua cgaauacuuu     2040

aauggaaaag acaacucaag ggaguggcag gaguucaagg cacguguuga aacgagucgc     2100

uuuccccgga gcaagaaaca gaggauucuc cugcagaagu ucgaugagga cggcuuuaaa     2160

gaaagaaauc uaaacgauac ccgauaugug aaucgguucc ugugucaauu ugucgccgac     2220

cgcaugagac ucacagguaa ggggaaaaag aggguguucg cuuccaacgg ccagaucacu     2280

aaccugcuuc ggggauucug gggcuugcga aagguaagag ccgagaauga ucgccaucac     2340

gcacuggacg cgguuguggu cgccugcucu accguggcua ugcagcagaa aaucacaagg     2400

uuuguccguu acaaggagau gaacgccuuc gaugggaaaa cuauugacaa ggaaaccgga     2460

gagguguuac aucagaaaac acacuuuccu cagccauggg aguucuuugc acaggaaguu     2520

augauacggg uguucgguaa gccggauggc aagcccgaau uugaggaggc ugacacccuc     2580

gaaaaacuga gaacucuucu ggccgagaag uugagcucaa ggccugaagc aguacacgag     2640

uaugucacgc cacucuucgu gagucgcgcc cccaaucgaa aaauguccgg gcagggacau     2700

auggaaacag uuaagagcgc uaaaagacug gaugagggcg ugucuguccu acgggugccu     2760

cugacccaac ucaagcugaa agaccuugag aagaugguaa acagggaacg cgagccaaag     2820

uuguacgaag cgcugaaagc ccguuuagag gcacacaagg acgaucccgc uaaagccuuu     2880

gccgaaccgu ucuauaagua cgacaaagcu gguaauagaa cucaacaggu gaaggcaguu     2940

cgggucgagc aagugcagaa gaccggggug ugggucagga accauaaugg caucgccgau     3000

aacgcgacaa ugguucgcgu ggacguguuu gagaaaggag auaaguauua ccucguaccu     3060

auuuacucau ggcaggucgc uaaaggcauc cugccagacc gagccguggu ucaggggaag     3120

gaugaagagg acuggcaacu uauugaugac aguuucaauu uuaaauucag ccugcacccc     3180

aacgaucuag uggaagucau caccaagaag gcaagaaugu ucggauauuu ugccuccugu     3240

caccggggca cugguaauau aaacaucagg auucaugacc ucgaucacaa aaucgggaag     3300

aauggcauuc uggagggaau cggugugaaa acagcuuugu cuuuccagaa guaccagaua     3360

gacgagcugg gcaaagaaau ucgcccuugc agauuaaaga agaggccccc aguucggaaa     3420

cguccugcag ccacgaagaa agcggggcag gcuaagaaaa agaaguaagg acuagucccu     3480

guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaagaauu                                                              3608


<210>  3657
<211>  4364
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3657
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaagaaa      180

ccauacacca uuggacugga cauagguaca aacagcguug gcugggccgu gcucacugau      240

caguaugacc uuguaaagag gaagaugaaa aucgcggggg auuccgagaa gaaacaaauu      300

aagaaaaauu ucuggggagu gcggcuguuu gacgaaggcc agacggcugc cgaucgccga      360

auggcacgua ccgccagaag gcggaucgag cgcagaagga accggauuuc uuacuugcag      420

gguaucuucg cugaagagau gucaaagaca gacgcaaacu uuuucugccg cuuaagugau      480

agcuuuuaug ucgacaauga aaagcgaaau uccagacauc cguucuuugc cacuauagag      540

gaggaagugg aguaccacaa aaacuauccc accaucuacc aucugaggga ggagcuaguu      600

aauucuucgg aaaaggcuga ucuccggcug guguaucuug cccuggcgca cauuaucaaa      660

uaccguggga acuucuugau ugaaggagca cucgacacac agaauaccuc agucgauggc      720

aucuacaagc aauuuauaca gacuuauaac cagguguucg ccagcggcau ugaggacggg      780

agucugaaaa agcuggaaga uaauaaagac guagcuaaga uccucguuga gaaggugaca      840

cgcaaagaaa aguuagagag gauucugaaa cuuuacccug gagagaaguc cgccgguaug      900

uuugcacagu ucaucucuuu gaucgucggc agcaaaggga acuuucaaaa gccauucgac      960

cugauugaaa aguccgauau agagugugcu aaagacucau augaagagga ucuagaaagu     1020

cuccuggccc uuaucggaga cgaguacgcg gagcuguucg uggccgcaaa gaaugcuuau     1080

agcgccgugg ucuugucuuc cauuaucacc guugcugaaa cggagacuaa cgccaaacuc     1140

ucagcaagca ugauagaaag guuugauacc caugaggaag accugggcga guuaaaggcc     1200

uucauuaaac ugcaccuccc caagcacuac gaggaaaucu uuaguaauac agagaagcau     1260

gguuaugcug gguacaucga uggaaaaacu aagcaggcgg acuucuacaa auauaugaag     1320

augacccuug aaaacauuga gggcgcagau uacuuuaucg ccaaaauuga gaaggaaaac     1380

uuccugcgga agcagcgaac auuugacaau ggcgcuauac cucaccaguu gcaucuagag     1440

gaacuggagg ccauccucca ccaacaggca aaauauuacc cauuccugaa ggaaaacuau     1500

gauaaaauua agucucuugu gaccuuucgc auccccuacu ucguagggcc ucuggccaau     1560

ggacaguccg aguuugcuug guugacgaga aaagccgacg gugagaucag gccauggaac     1620

auugaagaga agguggacuu cggcaaaagc gcagucgauu uuauagaaaa gaugacuaau     1680

aaggacacau aucucccgaa agagaacgug cugcccaagc acucguuaug cuaccaaaaa     1740

uaccugguuu auaaugaacu aaccaaggug cgguacauca acgaucaggg gaaaacuucu     1800

uauuucuccg gacaggagaa ggagcagauu uucaaugacc ucuuuaagca aaaacgcaag     1860

gucaaaaaga aagaucugga acuuuucuug agaaacaugu cacaugugga gaguccuaca     1920

aucgaaggcc uggaggacag cuuuaauucc ucuuacagca ccuaucacga ucuccugaag     1980

guagguauua agcaggaaau ccuugacaac ccaguuaaua cggagaugcu ggagaacaua     2040

gugaaaaucu ugaccgucuu cgaagauaag aggaugauua aagagcagcu ccagcaauuu     2100

ucagacgugc uggauggggu cguguuaaag aaacuggaac gucggcauua cacaggcugg     2160

ggacgacuaa gugcgaagcu ccugaugggc aucagagaca agcaguccca ccuuacuauu     2220

uuggauuauc ugaugaauga cgacgggcuc aacaggaacc ugaugcaguu aaucaaugau     2280

agcaaccugu cuuucaaauc aauaauugag aaggagcagg uuaccacagc ugacaaagau     2340

auccaaagua uuguggccga ccuugcagga agccccgcca ucaagaaagg uauauugcag     2400

ucccucaaga ucguagauga acuggucucu gugaugggcu acccuccaca gacuauuguu     2460

guggagaugg cucgcgaaaa ucagaccaca gggaaaggaa agaacaauuc gcggcccaga     2520

uacaagagcc uagagaaagc caucaaggaa uuuggcuccc aaauucugaa agagcauccg     2580

accgacaacc aggagcucag gaauaaccgc cuguaucuuu acuauuugca gaaugguaag     2640

gauauguaca cugggcaaga ccuggauauc cacaacuuau caaauuauga cauagaucac     2700

auugucccuc agaguuucau cacggacaac ucuaucgaua aucucgugcu gacaagcucc     2760

gcaggcaacc gagaaaaagg agacgacgua cccccacuug agauugugcg gaagcguaag     2820

gucuuuuggg aaaaacugua ccagggcaau uugaugucaa agagaaaauu cgauuaucuc     2880

accaaggcug agagaggggg acugacugaa gccgacaaag cgcgcuuuau ccaucggcag     2940

cuaguugaga ccagacaaau uacaaagaac guggccaaca uacugcacca gagguucaau     3000

uacgagaagg augaccaugg uaacaccaug aaacaggugc gaaucgucac ucucaagagc     3060

gcucugguuu cucaguuucg caaacaauuc cagcuuuaca aggugcggga ugugaaugac     3120

uaucaccacg cacaugaugc cuacuugaac ggcguagucg cuaauacacu guuaaaagug     3180

uauccucagc ucgaacccga guucguuuac ggggacuauc accaguuuga uugguucaag     3240

gccaacaagg caacggcgaa aaagcaauuu uacaccaaua uuaugcuguu cuuugcccag     3300

aaagacagaa ucauugauga aaacggagag auccuuuggg acaagaaaua ucuggacacu     3360

gugaagaagg ucauguccua caggcagaug aauauaguga aaaagacaga aauccaaaaa     3420

ggcgaguuca guaaggcuac cauuaaacca aaggguaaca gcucuaaacu aaucccccgu     3480

aagacaaauu gggauccuau gaaguacggg ggccucgacu ccccaaacau ggccuaugca     3540

guugugauug aguacgcuaa aggaaagaau aaacugguau uugaaaagaa aaucauacgc     3600

gucacuauua uggagcggaa ggccuucgaa aaggaugaga aagcauuuuu ggaggaacag     3660

ggcuauagac agccgaaggu gcuggccaaa uuacccaagu acacccucua ugagugugaa     3720

gaggggaggc gacggaugcu ggcuucagcg aacgaagccc agaaaggaaa ucaacaggug     3780

cuuccuaacc aucuggucac guugcuccac caugcagcua acugcgaggu uagugacggc     3840

aagagccugg auuacaucga gucgaaucgc gaaauguucg ccgagcuacu ggcccacgug     3900

uccgaauuug cuaagagaua uacccuugca gaggccaacc ucaauaaaau caaccagcug     3960

uucgaacaga auaaggaggg ugacauuaaa gcgauagcuc aaucuuucgu agauuugaug     4020

gccuuuaacg caaugggggc cccagcuagc uucaaguuuu ucgagacaac uaucgaaagg     4080

aaacgguaca auaaccugaa ggaguuacuc aauucaacca uuaucuacca gaguauuaca     4140

ggacuguaug aaucccgaaa gagacuugac gauaaacgcc ccgcagccac uaagaaagcg     4200

ggccaggcua agaaaaagaa auaaggacua gucccuguuc ccagagccca cuuuuuuuuc     4260

uuuuuuugaa auaaaauagc cugucuuuca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4364


<210>  3658
<211>  4397
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3658
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaagaaa      180

ccauacagca uuggacugga cauagguacc aacuccguug gcugggccgu gguaacagau      240

gacuauaagg ugccggcgaa gaaaaugaag guccucggga auacugauaa aucucauauc      300

gagaagaacc uucugggagc uuuguuauuc gacucaggca auacggccga agauaggcgg      360

cugaaacgca ccgcacgacg uagauacaca aggcggcgca accggauucu auaucuccag      420

gagaucuuua gugaagagau ggguaaggug gacgauagcu ucuuucacag gcuggaagac      480

uccuuccuug uuacugagga uaagagaggg gagcgccauc ccauuuuugg aaaucuggaa      540

gaggaaguga aauaccacga gaacuucccu accaucuauc auuugcgaca auaccucgcc      600

gacaauccag agaaggucga ucugagacug guguaucucg cuuuagcaca cauaaucaaa      660

uuuaggggcc acuuccugau ugaaggcaag uuugacacac ggaacaauga uguacagcgu      720

cuuuuccagg aguuuuuggc cguuuacgac aacaccuucg aaaauucuuc gcugcaggag      780

caaaacgugc aggucgaaga gauccuaacu gacaaaauuu caaagagcgc uaaaaaggau      840

cgcgugcuca agcuguuucc caaugagaaa aguaacggga gauucgccga guuccuuaag      900

cugaucgugg gaaaucaggc ggacuuuaaa aagcauuucg aguuggaaga gaaagcaccu      960

cuccaguuuu ccaaggauac auacgaagag gagcuggaag ucuuacuggc ccaaauaggu     1020

gacaacuaug cugaacucuu ccuuucugcc aagaaacugu acgauagcau uuugcuaucc     1080

ggcauccuga ccguuacgga cguggggacu aaggcaccac ucucagcuag uaugauucag     1140

agguauaacg aacaccagau ggaucuggcc cagcuuaaac aauuuauccg gcagaagcug     1200

agcgacaaau acaaugaggu auucucugau guguccaagg acggauaugc gggcuacauc     1260

gaugguaaga ccaaccagga agccuuuuau aaauacuuga aggggcuccu gaauaaaauu     1320

gagggaucag gcuacuucuu agacaagaua gagcgagaag acuuucugcg caaacaaaga     1380

acauucgaua acggcagcau cccccaucag auucaccuac aggagaugag ggcaaucaua     1440

cggcgccagg cugaauuuua uccuuuccuc gccgacaauc aagauagaau ugagaagcug     1500

cuuacuuuua ggaucccaua cuaugucggg ccguuggcuc guggaaagag ugacuucgcc     1560

uggcugucuc ggaaauccgc agauaagauc acccccugga acuucgacga gauuguggau     1620

aaagaaagcu cggccgaggc uuuuaucaau cgaaugacaa acuacgaccu cuaucugccu     1680

aaucagaagg uucuuccaaa acacucucug uuguacgaaa aguucaccgu guauaacgag     1740

cucacgaaag ucaaguacaa gacugaacag gguaaaacag cguuuuucga ugcaaauaug     1800

aagcaggaga uuuuugacgg cguguucaaa guauacagaa agguuaccaa agauaagcug     1860

auggacuuuu uagagaagga guucgaugaa uuuaggauag uggaccugac ugggcuagac     1920

aaagaaaaca aggucuucaa ugccuccuau ggaacauacc augaucucug caaaauccug     1980

gacaaggauu uucuugacaa caguaaaaau gagaagauuu uggaagauau cgugcugacc     2040

cucacgcugu ucgaggaccg cgagaugauc cggaagagau uagaaaacua uucagaucug     2100

cuuaccaaag agcaagucaa gaaauuggaa aggcgccacu acacaggcug gggucgacuc     2160

agcgcugagc ugauucaugg gauacggaac aaggaauccc guaaaacuau ccuagacuau     2220

cugauugaug acggcaauuc uaacagaaau uuuaugcagc ucaucaacga ugacgcccug     2280

agcuucaagg aggagauugc aaaggcccag gugaucggag aaaccgauaa ucuuaaccag     2340

guugugucag acauagcugg cagucccgcc aucaaaaagg ggauuuugca aucccugaaa     2400

aucguagacg aguuagucaa gauuauggga caccagccug aaaauaucgu gguugagaug     2460

gcaagggaga accaguucac aaaucagggu cgccggaaca gccaacagag acucaaaggc     2520

cugacugauu cuauaaagga auuuggguca cagauucuua aagagcaucc aguggaaaau     2580

agucaacugc agaacgacag guuguuccuc uacuaucugc agaauggacg agauauguac     2640

accggcgagg aacuagacau cgauuaccug agccaguaug acauugauca caucauaccc     2700

caagcguuua ucaaggacaa cuccauugau aaucgcgucc ucacaucuuc gaaggagaac     2760

cgggguaaaa gcgacgaugu gccguccaag gacguaguga gaaaaaugaa gucauacugg     2820

aguaaacugc uuucugcuaa guugaucacc cagaggaagu ucgacaaccu gacuaaagcc     2880

gagcgugggg gcuuaacgga ugacgauaag gcaggauuua uuaaacgcca gcucguugaa     2940

acacggcaga ucaccaagca cgucgccaga auacuggacg agagguucaa uacugaaacc     3000

gaugagaaca auaaaaagau ucgacaagug aagaucguga cacuuaaaag caaccugguc     3060

uccaauuuuc ggaaggaguu cgaacuauau aaaguucgcg agaucaacga cuaccaucac     3120

gcucaugaug ccuaucucaa ugcagugauu ggcaaggcuc uguugggggu guacccucag     3180

cuggaacccg aguuuguaua uggagacuac ccacacuucc acggucauaa agaaaacaag     3240

gccaccgcga agaaauuuuu cuacucaaau aucaugaacu ucuuuaagaa agaugacguc     3300

agaacugaua agaauggcga gauuauaugg aaaaaggacg aacacaucag caacauuaag     3360

aaaguguuau cuuauccuca gguuaauauc gugaagaaag ucgaggagca gacaggggga     3420

uucuccaagg aaaguauucu ccccaaaggc aacagcgaua agcugauccc aaggaaaacg     3480

aagaaguuuu acugggacac caaaaaguau gguggguucg acucucccau aguggccuac     3540

uccauccuug uuauugcuga uaucgagaaa ggcaagagua aaaagcugaa gacugugaaa     3600

gcauugguag gagucacaau uauggaaaag augaccuuug agcgggaccc uguggccuuc     3660

cucgagcgaa aaggcuauag aaaugugcaa gaagagaaca ucauaaagcu gccaaaauac     3720

ucacuauuua agcuggaaaa cgggcgcaag aggcuucucg cuagcgcccg ugagcugcag     3780

aaaggaaaug aaauugucuu gccgaaccau cugggcacau uacucuauca cgcaaagaau     3840

auccauaaag uugaugagcc caagcaccug gacuacgugg auaaacacaa ggacgaguuc     3900

aaggaacuuc uggauguagu gucgaacuuu uccaaaaagu acacuuuggc ggaggguaau     3960

cucgagaaaa ucaaggaacu guaugcccag aacaaugggg aagaccuaaa agagcuggcu     4020

ucuagcuuca uuaaccuucu caccuuuacg gccauaggag cuccugcaac cuucaaguuu     4080

uucgauaaaa auaucgaccg gaagagauac acaucaacua ccgagauucu gaacgccaca     4140

uugauccauc aaaguauuac uggcuuauau gaaaccagga ucgaucugaa uaagcucggu     4200

ggcgacaaac gcccagcagc cacgaagaaa gcugggcagg cgaagaaaaa gaaguaagga     4260

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4320

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aagaauu                                                    4397


<210>  3659
<211>  3530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3659
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcacagaca      180

acaaaccuga gcuacauucu cggacuugac cuggguauag ccuccguugg cugggcggug      240

guagagauca augaaaacga ggauccaauu ggguugaucg acgugggagu caggauuuuc      300

gaacgggcug aggugccgaa gacuggcgaa ucuuuagccc ugucacgccg acuagcacgu      360

aguacgagaa ggcucauccg gcgcagagcc cauaggcugc uucuggcuaa acgguuuuug      420

aagcgcgagg guauacucag caccaucgau cuggagaagg ggcugcccaa ucaagcaugg      480

gaacuccgag uugccggauu agagagaagg cuguccgcua uugaaugggg cgccgugcuu      540

uugcaccuga ucaaacaucg gggcuaucua ucuaagcgua aaaacgaguc gcagacaaau      600

aacaaggagc ucggggcgcu gcuuucagga gucgcacaga aucaccagcu guugcaaagc      660

gacgauuacc gcacuccugc cgaacucgcu cugaaaaagu ucgccaagga ggaaggucau      720

auuagaaacc agaggggcgc auauacccac acauuuaauc gguuagaccu gcucgcugag      780

cuuaaccugu uguucgccca gcagcaccaa uuugggaauc cacauugcaa agaacacauc      840

cagcaguaca ugaccgagcu acugaugugg cagaagcccg cgcucagugg agaggccaua      900

cugaaaaugc uuggcaagug uacucaugag aaaaacgagu ucaaggcagc uaaacacaca      960

uauuccgccg aacgauuugu guggcugacc aaguugaaua accuccgcau ucuggaggau     1020

ggugcugaaa gagccuuaaa ugaggaggaa aggcaacugc uaaucaacca cccuuacgag     1080

aagucuaaac ucacguaugc acagguacgg aagcugcuug gguugagcga acaggccauu     1140

uucaaacauc ugcgcuacuc caaggagaac gcugaaucag cgacuuuuau ggagcucaaa     1200

gcauggcacg ccaucagaaa ggcucuggag aaucaaggac uuaaggacac cuggcaggau     1260

cuggccaaaa agccagacuu gcucgaugaa aucggcacag cauucagucu guacaaaacu     1320

gacgaggaua uucagcagua uuuaaccaac aagguuccca auagcgugau aaacgcccug     1380

cuagucucuc ucaauuuuga caaauucauc gaacuguccc uuaagucauu gaggaagauu     1440

cugccucuca uggagcaagg caaacguuac gaccaggcuu gccgggagau cuaugggcau     1500

cacuacggag aagccaacca gaagacaagc cagcuguuac cagcaauacc ggcgcaagag     1560

auucgaaauc ccguggugcu gagaacccuu agucaggcua ggaaagucau caacgccauc     1620

auucgccagu augguucucc ugcacggguu cauaucgaaa cgggcagaga guuggggaag     1680

uccuuuaaag aaaggcgcga gauucagaag caacaggagg auaaucgaac uaagcgggaa     1740

agcgccgugc agaaauucaa ggaacucuuc ucggacuuuu cuuccgaacc aaaaucaaag     1800

gauauacuga aauuccgucu auacgagcag caacacggaa agugucugua uaguggcaaa     1860

gaaaucaaca uucacagacu caaugagaag gguuacguag agaucgacca ugcucugccc     1920

uuuagcagga caugggauga cuccuucaac aauaaggugc uugucuuggc cucugaaaac     1980

cagaauaaag ggaaccagac cccuuacgag uggcugcaag gcaagaucaa cagcgaacgc     2040

uggaaaaauu uuguggcauu aguucucgga ucacagugca gugcugccaa gaaacagcgg     2100

cugcuuacuc aggugauuga ugacaacaag uucauagaua gaaaucugaa cgacacaagg     2160

uauaucgcgc gauuuuuguc caauuacauu caagagaacc uccugcuagu cggcaagaau     2220

aaaaagaacg uguucacccc aaaugggcag aucacggccc ugcuccgcag ccggugggga     2280

cugauuaaag cuagagaaaa caauaacagg caccaugcac uugaugccau cguaguugcu     2340

ugugccaccc ccucuaugca gcagaagaua acacguuuua uccgcuucaa agaggugcac     2400

ccguauaaga uugagaaucg guacgaaaug gucgaccaag agucagguga aaucauuagu     2460

ccucacuuuc ccgagccaug ggcauauuuc agacaggagg ugaacaucag ggucuuugac     2520

aaucauccug auacuguguu gaaggaaaug cugcccgacc gaccacaggc gaaccaccag     2580

uucguucaac ccuuauucgu gagccgggcc ccuacccgca aaauguccgg ccaggggcau     2640

auggagacaa uaaagucugc uaaaagacuc gccgaaggaa uuucgguacu gaggauccca     2700

cuuacucagc ugaagccgaa ccuacucgag aauaugguca acaaagaacg ggagcccgca     2760

cuguacgcug gcuugaaggc ccgacuggca gaguuuaauc aagauccugc caaggcuuuc     2820

gcgaccccau uuuauaaaca gggugggcaa caggugaagg ccauuagagu ugaacaggug     2880

cagaaaagcg gcgucuuagu gcgcgagaac aauggaguag cagacaacgc uuccaucgug     2940

aggacagaug uuuucauaaa gaauaacaaa uuuuuccucg uccccaucua caccuggcaa     3000

guggccaagg gcauucugcc uaauaaagcc aucguggcuc acaagaacga agacgagugg     3060

gaagagaugg augagggggc aaaguuuaaa uucucacuuu uuccaaauga ccuggucgaa     3120

uugaagacua aaaaggagua cuucuuugga uauuacauug gucucgaucg ugccacgggc     3180

aacaucaguc ugaaagaaca ugacggggag auaucuaagg gaaaggaugg cguuuaucgg     3240

guggguguga aacuagcgcu gagcuucgaa aaguaccagg uagacgagcu ugggaaaaau     3300

agacagauuu gcaggcccca gcagcgccag ccgguccgaa agagaccugc ugccaccaaa     3360

aaggcaggcc aagccaagaa aaagaaauaa ggacuagucc cuguucccag agcccacuuu     3420

uuuuucuuuu uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                3530


<210>  3660
<211>  3608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3660
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcagccgcg      180

uucaagccaa acccgauuaa uuacauacug ggacucgaca ucgguauugc uagcguuggc      240

ugggccaugg uggagaucga ugaagacgag aaccccauuu gccuuaucga ucugggggua      300

aggguguuug aacgggcaga ggucccuaaa accggagacu ccuuggccau ggcucgccga      360

uuagcacguu cugugagaag gcugacacgg cgcagagccc auaggcuacu ccgggcucgc      420

cgacugcuua agagagaagg cguucugcag gccgcggauu ucgacgagaa ugguuugaua      480

aagucacucc caaacacucc cuggcaacug agggcagccg cucuggaucg gaaacucacg      540

ccuuuagagu ggagugccgu gcugcuucac uugaucaagc aucgugggua ucugagccag      600

cgcaaaaaug aaggagagac cgcagacaag gaacuaggcg cucuccugaa aggcgucgcc      660

gauaacgcgc acgcccuuca gacaggggac uuuagaacuc cagcagagcu ggcuuugaau      720

aaguucgaga aggaauccgg acauauuagg aaccagcggg gugauuacuc ucacaccuuu      780

ucgcgaaaag accuccaagc cgagcugauc uuacuguucg aaaagcagaa agaguuuggc      840

aauccccacg ugucaggggg acucaaggaa ggcauugaga cacuucugau gacccagcgc      900

ccugcuuuga gcggugaugc cguacagaaa augcuagggc auuguacuuu cgagccagca      960

gaaccgaagg ccgcuaaaaa cacauauacc gcggagagau uuaucuggcu gacgaagcuc     1020

aauaaccuga ggauacuuga acaaggaagu gagcggcccc ugacugacac cgaacgcgca     1080

acauugaugg acgagccuua cagaaagucc aaacucacuu augcccaggc uaggaagcug     1140

uuaggccugg aggauaccgc cuucuuuaaa ggccuacguu acgggaagga caaugcagaa     1200

gccucuacac ucauggagau gaaggcuuau cacgccauua gccgggcacu ggaaaaggag     1260

ggacuuaagg auaaaaaguc cccauugaac cugucacccg aacuccagga cgagaucggu     1320

accgcguuca gucuguuuaa aacggaugag gacauuacug gccgacuuaa ggauagaauc     1380

cagccugaaa uccuggaggc uuugcucaaa cauauuagcu ucgacaaguu cguucaaaua     1440

ucucugaagg ccuuaaggcg caucgugcca cugauggaac aggggaaacg guacgaugag     1500

gcaugcgccg agauuuacgg agaccacuau ggcaagaaaa auacagaaga gaagaucuac     1560

cuacccccga uaccugcuga ugaaauuaga aaccccgucg ugcucagggc ccugucccag     1620

gcacgcaaag ugaucaacgg ugucguucga cgguaugggu caccagcucg uauccacauu     1680

gagaccgcca gagaaguggg caagagcuuu aaggacagga aagagaucga gaagcgccaa     1740

gaagagaauc ggaaagacag agaaaaggcg gccgcuaaau ucagggagua cuuuccuaac     1800

uucguaggag aacccaagag uaaagauauu cuuaaguugc gacuguauga gcagcagcau     1860

ggcaaguguc ucuacucugg gaaagagaua aaucugggac gcuuaaacga aaaggguuau     1920

guggagaucg accacgcacu gccauuuucc cggacuuggg augacagcuu caauaacaaa     1980

guccuugugu ugggcucgga aaaucagaac aaggggaauc aaacacccua cgaguacuuu     2040

aacggaaaag auaauucuag agaauggcag gaguucaagg ccaggguuga gaccucccgu     2100

uuuccucgcu caaagaaaca gcggauucuc cugcagaagu ucgacgaaga uggcuuuaaa     2160

gagagaaacc uaaaugacac gagguaugug aaccgauucc ugugccaauu ugucgcugau     2220

cggaugcgcc ucaccgguaa ggggaaaaag agaguguucg ccaguaaugg ccagaucaca     2280

aaccugcuua ggggauucug gggcuugcgg aagguacgag cagaaaacga cagacaucac     2340

gcgcuggaug ccguuguggu cgcuuguagc acuguggcca ugcagcagaa aaucacccgc     2400

uuugucaggu acaaggagau gaaugcauuc gacgggaaaa caauugauaa ggagacugga     2460

gaaguguuac aucagaaaac ccacuuucca cagccguggg aguucuuugc ucaggaaguu     2520

augauacgug uguucgguaa gcccgacggc aagccugagu uugaagaggc cgacacacca     2580

gagaaacucc ggacccugcu ugcagaaaag cuguccucua gacccgaggc cguacacgaa     2640

uaugucacuc cuuuguucgu gagcagggcu ccaaaccgca aaaugucagg gcagggacau     2700

auggagacgg uuaagagugc gaaacgacuc gaugaaggcg uguccguccu gagagugccc     2760

cuaacacaac ugaagcucaa agaccuggag aagaugguaa aucgggagag ggaaccgaaa     2820

cuuuacgagg ccuugaaagc acgccuggaa gcucacaagg augacccugc caaagccuuu     2880

gcugagccau ucuauaagua cgauaaagca gguaaccgua cccaacaggu gaaggccguu     2940

agagucgaac aagugcagaa gacuggggug uggguccgga aucauaacgg caucgcggac     3000

aaugcuacca ugguuagggu ggauguguuu gagaaaggag acaaguauua cuuaguaccc     3060

auuuacagcu ggcaggucgc caaaggcauc cucccugauc gcgcaguggu ucaggggaag     3120

gacgaggaag auuggcaacu gauugacgau ucuuucaacu uuaaauucuc acuucacccc     3180

aaugaccugg uggaggucau cacaaagaag gcccgaaugu ucggauauuu ugcuaguugc     3240

cacagaggca ccgguaacau aaauauccgg auucaugacc uagaucacaa aaucgggaag     3300

aacggcauuc ucgaaggaau cggugugaaa acugcacuga gcuuccagaa guaccagaua     3360

gacgaguugg gcaaagagau uaggccaugu cgccugaaga agagaccucc cguuaggaaa     3420

cggccagccg cgacaaagaa agcugggcag gccaagaaaa agaaguaagg acuagucccu     3480

guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaagaauu                                                              3608


<210>  3661
<211>  3614
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3661
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggcac ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaaaguac      180

cacguuggaa uugacguggg uaccuucagc guaggccugg ccgcgauaga gguggaugac      240

gcugggaugc caaucaaaac acucucccuu gucucucaca uucaugauuc aggacuggac      300

ccggaugaaa ucaagagugc cgugacuagg uuggcaagcu ccggcauugc ccggcgcacg      360

cgacguuuau auagaaggaa gcggcgcaga cugcagcaac uagacaaauu uauccagagg      420

caggguuggc ccguuauaga gcucgaagau uacucugacc cucuguaucc auggaaggug      480

cgggcugagc uugcagccuc guacaucgcu gaugaaaaag agcgcgggga gaagcuguca      540

gucgccuugc gacacauugc gagacauagg ggauggcgga accccuaugc aaaagugagc      600

agucucuacc ugccugacgg cccauccgau gccuucaagg cuauccguga agagauuaag      660

cgcgccucug gccagcccgu accugaaacc gcaacaguug ggcaaauggu gacucugugc      720

gagcucggaa ccuuaaaacu gagaggugag ggcggggucc uuagcgcuag guugcagcag      780

uccgacuaug cccgggaaau ccaggagaua ugucgaaugc aagaaauugg acaggagcug      840

uaccgcaaga ucauugaugu gguguuugcg gccgaaucac caaaaggcag ugcaagcucu      900

agagucggua aggacccgcu acagcccggg aaaaauaggg cucucaaggc cuccgacgcu      960

uuccagcggu accgcaucgc cgcacugauc ggaaaccuua gaguuagggu ggauggcgag     1020

aaacguauuc ugucaguaga ggaaaagaau uugguguuug accaccucgu caaccugaca     1080

ccuaagaaag agccagaaug ggugaccaua gccgagaucu uaggcauuga ucgggggcaa     1140

cugaucggaa cugcuacaau gaccgacgau ggugaacgag cgggcgcaag acccccuacg     1200

cacgacacua auaggagcau aguuaacagu cgcauugccc cacucgugga uugguggaag     1260

accgcuucug cccuugagca gcaugcaaug gucaaagccc uguccaaugc ugagguggac     1320

gauuucgaca gccccgaagg ggccaaggua caggcauuuu ucgcggauuu ggacgacgau     1380

guucacgcua aacuagacuc gcugcaucuc ccggugggac gggccgcaua uucugaggau     1440

acacugguca gacuuacuag gcgcaugcug uccgacggcg uggauuugua caccgcccga     1500

cuccaagaau uugguaucga gccuucaugg acacccccaa ccccucggau cggggaaccc     1560

gucggcaacc cagcugugga ccguguucug aagacgguga guagaugguu agagagcgcc     1620

acuaagacau ggggagcacc cgagagggua auuaucgaac acguccgcga gggcuucgug     1680

accgaaaaac gggcuagaga gauggauggg gacaugaggc gacgcgccgc gcggaaugcc     1740

aagcuguuuc aggagaugca ggaaaaacua aacguucagg gaaagccuuc cagagcugau     1800

cucuggaggu aucaaucugu gcagcgucag aauugccagu gugcauacug cgguagccca     1860

auuacuuucu caaacaguga gauggaccac auagucccgc gcgccggcca aggguccaca     1920

aauacccggg aaaaccuggu ggcuguaugu cauagaugca aucagagcaa aggaaacacg     1980

cccuuugcca ucugggcaaa gaacaccucu auugagggcg ugucaguuaa ggaagcgguc     2040

gagaggacac gacacugggu gacugauacc gguaugcgga guacagacuu caaaaaguuc     2100

acuaaagccg uggucgagcg cuuucagaga gcuaccaugg augaagagau cgacgccagg     2160

agcauggaau ccguugcaug gauggcuaau gagcuucggu cucgaguggc ccagcauuuc     2220

gcaucgcacg ggacaaccgu gagaguauau cgcggcagcu ugacugccga agcuaggcgu     2280

gcguccggaa ucucaggcaa gcugaaauuu uucgacgggg ucggaaagag ucggcucgau     2340

agaaggcauc acgccauuga cgcagcugug auagccuuua cgucugauua cguugccgag     2400

acacuggcug ugcgcagcaa ccuuaagcaa ucccaggcac accgacagga ggccccucag     2460

uggagagagu ucaccgguaa agacgcggaa caucgggcug ccuggagggu cuggugucaa     2520

aagauggaaa aacugucagc auugcucacu gaggaucugc gcgacgaucg ugugguugug     2580

augagcaaug uaagauuacg gcugggcaac gggucugccc acaaggaaac caucggaaaa     2640

cuauccaagg ucaaacucag uagccagcug ucuguguccg acauugauaa ggcuucaagu     2700

gaggcacuuu ggugcgccuu gacaagggag ccaggcuuug accccaagga aggucugccu     2760

gcgaauccag agcgccauau ccgagugaac gggacccacg ucuaugcugg cgauaauauu     2820

ggacucuucc ccguuagcgc cggcucgauc gcacugagag ggggauacgc cgaauuaggc     2880

uccucuuuuc accaugcucg gguguacaaa auaacuagcg guaagaaacc ggccuucgca     2940

augcugaggg uauauacaau cgaccuuuug ccuuaccgca accaggaucu cuuuucagug     3000

gagcugaagc cacaaacgau gaguaugaga caggcugaga aaaagcuaag ggacgcccug     3060

gcgaccggga augccgaaua ucucggaugg cuggucgugg acgaugagcu uguuguggac     3120

acuuccaaga uugcaacaga ucaggucaaa gcuguggaag ccgaguuggg caccauccgg     3180

cguuggcgcg uugacgguuu cuuuucuccc agcaagcugc gauuaagacc ucuccagaug     3240

uccaaagaag gcauuaagaa agagucagcu cccgagcuga gcaagaucau agauaggcca     3300

ggguggcuuc cugcagugaa caagcuguuc agugacggaa augucacagu agugcggcgc     3360

gauucuuugg gcagaguuag gcucgaaucc acugcccauc ugcccgugac cuggaaaguc     3420

caaaagcggc cagccgcuac gaaaaaggcg gggcaggcca aaaagaagaa auaaggacua     3480

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     3540

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaag aauu                                                       3614


<210>  3662
<211>  3314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3662
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcagccagg      180

auucuggcgu ucgacauagg aaucagcucc auugguuggg cuuuuucuga gaacgaugaa      240

cucaaggacu gcggcguucg gaucuucacc aaaguggaga auccaaagac aggggaauca      300

cuugcccugc cgcgccgauu ggcacguagu gccagaaaga gguuagcucg gcgcaaagca      360

agacugaacc aucuaaagca ccucauugcc aaugaguuua aacugaacua cgaagauuau      420

cagagcuucg acgagucccu ugcuaaggcc uacaaaggau cucugaucuc gcccuaugag      480

uugagguuuc gggcgcucaa ugaacugcug ucaaagcaag auuucgcacg cguaauacuc      540

cauaucgcca agcgaagagg cuacgacgau auuaaaaaca gcgacgauaa ggagaaaggu      600

gcuaucuuaa aggccauuaa acagaaugaa gagaagcugg caaacuauca gagugugggg      660

gaguaccuuu auaaagaaua cuuucagaag uucaaggaaa auuccaaaga auuuacuaac      720

gucaggaaua agaaagaguc uuacgaacgg uguaucgcuc agagcuucuu gaaggacgag      780

cugaaacuaa uauuuaagaa gcaacgugag uucggauuuu ccuucucuaa aaaguucgaa      840

gaggaagugc ucaguguugc cuuuuauaaa cgcgcgcuga aggauuucag ccaccuugug      900

ggcaacugcu cauuuuucac ggacgagaaa agagccccua aaaauucccc acuggcauuu      960

auguucgucg cuuugaccag gauuaucaac cuccugaaua acuuaaagaa cacagaaggc     1020

auucuguaca cuaaagauga ccucaaugcc cuucugaacg agguguugaa gaaugggacc     1080

cuaacauaua aacagaccaa gaaacugcuc ggacugucag acgauuacga guuuaagggu     1140

gaaaagggca cuuauuucau cgaguuuaaa aaguacaaag aguucaucaa ggcucuuggg     1200

gagcauaacc ugagccagga cgauuugaau gaaauugcca aagacauaac acucaucaag     1260

gaugagauua aacugaagaa ggcauuagcc aaauaugacc ugaaccaaaa ucagaucgau     1320

agucuaucua agcucgaguu uaaagaccac cugaacauau ccuucaaggc ucuuaaauug     1380

guaacucccc ugaugcucga aggaaagaag uacgaugagg cguguaauga acugaaccuu     1440

aaaguugcaa uuaaugagga caagaaagau uuucugccug ccuucaacga gacguacuau     1500

aaggacgaag ugacuaaucc agucguguug cgggcuauca aagaguaccg aaaggugcuc     1560

aacgcccugu uaaagaaaua uggcaagguc cacaaaauca auauugaacu ggcacgcgag     1620

guugguaaga accauagcca gagagccaaa aucgaaaagg agcagaacga gaauuacaag     1680

gcuaaaaagg acgccgaacu agagugcgaa aaacucgggc ugaagauuaa cucgaaaaau     1740

auacuuaagu ugaggcuguu caaagagcaa aaggaauuuu gugcauauuc uggagagaag     1800

aucaaaauuu ccgaucucca ggacgagaag augcuggaaa ucgaucacau cuaccccuau     1860

ucacggaguu ucgacgauag cuacaugaac aaaguguuag uauuuaccaa gcagaaucaa     1920

gagaaacuga accagacacc uuucgaagcg uuuggcaaug acuccgcuaa guggcagaag     1980

auugaggugc uugccaaaaa cuugccaacu aagaaacaga agcgcauacu cgauaaaaau     2040

uacaaggaca aggaacaaaa gaacuucaag gauagaaauc ugaacgacac cagguauauc     2100

gcacgucuag uccugaauua cacaaaagau uaucucgacu uucugccgcu uucugaugac     2160

gagaacacca aguugaauga cacgcagaaa ggcagcaagg ugcauguuga ggccaaguca     2220

gggaugcuga ccagugcuuu acggcacaca uggggauucu ccgccaaaga ucgaaacaac     2280

caccuccauc acgcaauuga cgcugugauc auugccuacg cgaauaacag caucgucaag     2340

gccuuuucug auuucaaaaa ggaacaggag ucaaauagug cugaacugua ugccaaaaag     2400

auaagcgagc uugacuacaa gaacaaaaga aaguuuuucg agcccuuuuc cgguuucagg     2460

cagaaagugc uggauaagau cgacgaaauu uucguaucua aaccugagcg caagaaacca     2520

ucgggcgcau ugcaugaaga gaccuuucgg aaggaagagg aguucuauca aagcuacggg     2580

ggaaaggaag gcguucucaa agcucuggag cuagguaaga ucagaaaagu gaaugggaag     2640

auugucaaaa acggcgauau guuuagggug gacaucuuca agcacaagaa aacuaauaag     2700

uuuuacgccg uccccauaua uacaauggau uucgcacuga aagugcuccc uaacaaggcg     2760

guugcccgcu ccaaaaaggg agaaauuaag gacuggaucc ugauggauga gaauuacgaa     2820

uuuugcuucu cacuuuauaa agacaguuug auucugaucc agaccaagga uaugcaggag     2880

ccagaguuug uguacuauaa cgcuuucacg ucuagcaccg uauccuuaau agucucaaaa     2940

caugacaaua aguuugaaac acucagcaaa aaccagaaga uccuguucaa gaaugccaac     3000

gagaaagaag ugauugcaaa gucuaucggc auucaaaauc uuaaaguuuu cgagaaguac     3060

aucguguccg cucuggggga agucacuaaa gccgaguuuc gacagcggga ggacuucaag     3120

aaaaagcguc ccgcagccac caagaaagcu ggacaggcga agaaaaagaa auaaggacua     3180

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     3240

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3300

aaaaaaaaag aauu                                                       3314


<210>  3663
<211>  3725
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3663
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaagcgac      180

cugguucucg gacuugauau ugguauaggc uccguggggg uaggaauccu gaacaaggug      240

accggcgaga uuauccauaa aaauucuagg auuuucccag ccgcgcaggc ugaaaacaau      300

uugguccggc gcacaaaccg acaaggucgu agauuagcca ggcggaagaa gcaccgcaga      360

gugaggcuga aucggcuauu ugaggaauca gggcucauca cugacuucac gaaaauaagu      420

aucaaccuga auccguacca gcuucgcguu aagggacuga ccgaugaguu gagcaacgaa      480

gaacucuuua uugcacugaa aaauauggug aagcaucgag gcaucuccua ucuggacgau      540

gccucugacg auggcaacuc gucagucggg gacuacgcuc agauugugaa agagaauagc      600

aagcagcucg aaacaaagac ucccggacaa auccaguuag agagauauca gaccuacggu      660

cagcugaggg gcgauuucac aguagaaaaa gacgggaaga aacaccggcu uauaaacguu      720

uuuccuacca gugcauaucg uuccgaggcc uugcgcauuc ugcaaacuca gcaggaguuc      780

aauccacaga ucacagauga auuuauuaac agauaccuag agauccucac cggaaagagg      840

aaauauuacc auggccccgg uaaugaaaag ucucggacgg acuacgggcg auaucgcacu      900

agcggagaga cccuggauaa caucuucggc auucuuauag gcaaaugcac auuuuacccu      960

gacgaguuca gagcugccaa ggcguccuau acugcacaag aguuuaaccu guugaaugac     1020

cucaacaauc ugaccgugcc aacagagacc aagaaauuau caaaggaaca gaaaaaccag     1080

aucauuaauu acgucaagaa cgagaaagcc auggggcccg cuaagcuguu caaguauauc     1140

gccaaacucc uuaguuguga uguggcagac auaaagggau acaggauuga uaaaagcggu     1200

aaggcugaaa uccacacguu ugaggccuau cggaaaauga agacucugga aacauuggac     1260

aucgagcaaa uggaucgcga gacccuagac aagcuggcgu acgugcucac ucugaauaca     1320

gaaagagagg gcauucagga agcccuugag cacgaguucg cagauggguc uuucucccag     1380

aaacaggucg acgagcuggu ucaauuuagg aaggcuaacu caagcaucuu cggaaaaggc     1440

uggcauaauu uuagugugaa guugaugaug gagcucauuc cugaacugua cgagaccucu     1500

gaagaacaga ugacgauauu aacccgucug gguaaacaga agacaacuuc cagcucgaac     1560

aagaccaaau auaucgauga gaagcuacuc acagaagaga uuuacaaucc aguaguggcc     1620

aaaucugucc ggcaggcuau caagaucgug aacgccgcaa uuaaagaaua uggggacuuc     1680

gauaauauag uuaucgagau ggcccgagaa acuaacgagg acgacgagaa gaaagcuauu     1740

caaaagaucc agaaggcgaa uaaagaugaa aaggacgcag ccaugcugaa agcugccaac     1800

caguacaacg gcaaggcaga gcuuccgcac uccguguuuc auggacacaa acaguuggcc     1860

accaagauua gacuguggca ccaacagggc gaaaggugcc ucuauacagg gaagaccauc     1920

ucaauacaug aucugaucaa uaacaguaau caguucgagg ucgaccacau ucuuccccug     1980

agcaucacuu uugaugacuc cuuggcuaac aaagugcucg uauacgccac ggcaaaucag     2040

gaaaagggac aacgcacacc uuaucaggcg cuggauucua uggacgaugc uuggagcuuc     2100

cgggaguuaa aagccuuugu uagagaguca aagacccuga guaacaaaaa gaaggaauac     2160

cuacucacug aggaagacau uuccaaauuc gaugugagga agaaauuuau cgagcgcaau     2220

cuggucgaca cccgauacgc aagccgggug guccuuaacg ccuugcagga acauuuccgu     2280

gcucacaaga uagauacaaa agugucuguu gugagagguc aauuuaccuc acagcugagg     2340

cgccauuggg gcauugagaa gacucgggac acauaucacc accaugccgu agacgcacuc     2400

aucauugcug ccaguagcca gcugaauuua uggaagaaac agaagaacac gcuggucucc     2460

uacucugagg aucaacuuuu ggacaucgaa accggggagc ucauaucgga ugacgaauau     2520

aaagagagcg uguucaaggc gccauaccag cacuucguug auacucugaa auccaaggag     2580

uuugaagacu caauccuauu caguuaucag guggauucua aauuuaauag aaagauuagc     2640

gacgccacaa ucuacgcuac caggcaggca aaggucggaa aagauaaggc cgacgagaca     2700

uaugugcugg gcaaaauuaa ggauaucuac acucaagacg guuacgaugc uuucaugaaa     2760

auauauaaga aggacaaauc caaguuucuc auguaccgac augaccccca gaccuucgaa     2820

aaaguaauug agccuauccu ggaaaacuau ccaaauaagc agaucaacga gaaagggaag     2880

gaggugcccu guaauccguu ucuuaaguac aaagaggaac acggcuauau ucgcaaguac     2940

agcaaaaagg gaaacggccc ugaaaucaaa ucauugaagu auuacgauuc uaagcugggg     3000

aaccacauug acauaacgcc caaagauucc aauaacaagg uugucuuaca gagugugagc     3060

ccauggcggg ccgacgugua cuucaauaaa accacaggaa aguaugagau ccucggucug     3120

aaauacgcag aucuucaauu ugaaaagggc acugggaccu auaagauuuc ucaggagaaa     3180

uacaacgaca ucaagaaaaa ggagggaguc gauuccgacu cagaguucaa auuuacacug     3240

uauaagaaug auuugcuccu gguuaaagac acugaaacca aggaacagca acuauucaga     3300

uuucugagua ggacgaugcc uaagcagaaa cauuacgugg agcucaagcc cuaugauaaa     3360

cagaaguucg aaggcgguga ggcgcugauu aaagugcuug ggaacguagc caauagcggc     3420

cagugcaaga agggauuggg caaaucgaac aucuccauau acaagguccg uacagacgug     3480

cuggggaauc aacacaucau uaaaaacgag ggagacaagc caaaauuaga uuucaagcgc     3540

cccgcugcca ccaagaaagc aggccaggcu aagaaaaaga aauaaggacu agucccuguu     3600

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

gaauu                                                                 3725


<210>  3664
<211>  4283
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3664
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaacccag      180

uucgagggau uuacaaaccu guaccaaguu agcaagacuc ucagguucga acuuauucca      240

caggguaaaa cgcugaagca uauacaggag cagggcuuua ucgaagagga caaggcccgg      300

aaugaucacu auaaagaauu gaagccgauu aucgaccgca uuuacaaaac cuaugcggau      360

caaugcuuac agcuggugca gcuagacugg gagaaccucu ccgcugccau cgauucuuac      420

cgaaaggaga aaacagaaga gacucguaau gcacugauag aggaacaggc caccuauaga      480

aacgcuaucc augacuacuu cauugggagg acagauaauc uuaccgacgc aaucaacaag      540

cggcacgccg agauuuauaa gggacuguuu aaagcugaau uguucaaugg caagguacuc      600

aaacaacugg guacugugac aaccacggag caugaaaacg cccugcuccg cucauuugau      660

aaguucacua ccuacuuuag uggguucuac gagaauagga aaaacgucuu uagcgcggaa      720

gacaucucca cagcaauacc ccacagaauu gugcaggaua auuucccuaa guuuaaagag      780

aacugucaca ucuucacucg guuaauuacc gccguuccau cucugcgcga gcauuucgaa      840

aaugugaaga aggcuaucgg aaucuuuguc ucgacaucaa uugaggaagu guucagcuuu      900

cccuucuaua accagcuuuu gacccagacg caaauagacc uguacaauca gcuacucggc      960

ggcaucaguc gagaggccgg gacugagaaa auuaagggac ugaacgaagu acuuaaccug     1020

gcaauccaga aaaaugauga gacagcucac auaauugccu ccuugccuca uagauuuauc     1080

ccacucuuca agcaaauccu gucugacagg aacacccuua gcuuuauucu ggaagaguuc     1140

aaauccgacg aagagguuau ccagucauuu ugcaaguaua agacucuccu ucggaaugaa     1200

aacgugcugg agacagcgga ggccuuguuc aaugaacuaa acaguauuga ucugacccac     1260

auauuuauca gccacaaaaa gcucgagacg auuucuuccg cacuguguga ccauugggau     1320

acccuucgua augcucugua cgaacgcaga aucucagagu ugacagguaa aaucacuaag     1380

agcgccaaag agaaggucca gaggagucuc aagcacgaag acauuaaccu gcaggagaua     1440

aucucugcug ccggcaaaga auuauccgag gcauucaagc agaaaaccag cgaaauucug     1500

ucgcaugccc acgcugcgcu agaucaaccc cucccuacaa cucugaagaa acaggaggag     1560

aaggaaaucc uuaagucuca guuggacucc cugcucgggc uguaucaucu ucuggauugg     1620

uuugcagugg acgagucaaa ugaaguggau ccagaguuca gugcccgguu gaccggaauu     1680

aaacucgaaa uggagccgag ccuguccuuc uacaacaagg cucgaaauua ugccacuaaa     1740

aagcccuacu cugucgagaa auuuaaguua aacuuccaaa ugccuacccu ggcaagcggc     1800

ugggacguua auaaagaaaa gaacaauggu gccauccuau uugugaagaa cgggcucuac     1860

uaucugggaa uaaugccaaa acagaagggc cgcuacaaag cucuuucauu cgagcccacu     1920

gaaaagacga gugagggcuu ugauaaaaug uauuacgacu auuucccuga cgccgcaaag     1980

augaucccaa agugcuccac acaguugaaa gcgguaaccg cucacuuuca gacucacacc     2040

acacccauuc ugcucagcaa caauuucauc gaaccgcugg agauuaccaa ggagaucuac     2100

gauuuaaaca auccugaaaa agagcccaag aaauuucaaa cugccuaugc aaagaagaca     2160

ggggaccaga aaggauacag agaagcccug uguaagugga uagauuucac gagggacuuu     2220

cuuucuaaau acaccaagac uacaucaauu gauuugagua gccuccggcc auccucucag     2280

uauaaagacc ugggugagua cuaugcugag cuaaacccuc ugcucuacca uaucucguuc     2340

cagcgcauug ccgaaaagga gaucauggau gcaguggaaa ccggcaagcu guaucuuuuu     2400

caaauauaca auaaagacuu cgcuaagggg caccauggaa aacccaacuu gcacacacug     2460

uauuggacug gcuuauucag cccagagaau cucgccaaga ccuccaucaa acugaacggu     2520

caggcggaau uauuuuacag acccaaguca aggaugaaac guauggccca ccggcugggg     2580

gagaagaugu ugaauaagaa acucaaggau cagaaaacgc cuauuccaga cacccuguac     2640

caagagcuau augauuacgu caaccaucga cugagucacg accucucuga ugaagcuaga     2700

gcacugcuuc cgaaugugau cacaaaggag guuagccaug aaauuaucaa agacaggcgc     2760

uucacuuccg acaaguuuuu cuuucacgug cccauaaccu ugaacuauca ggccgcuaau     2820

ucaccuagca aguucaacca gcgggucaau gccuaccuga aagagcaucc agaaacaccc     2880

auuaucggca ucgauagagg agagaggaac uuaauuuaua ucacugugau ugacucuacc     2940

ggcaagauac ucgagcagcg cucccugaac acgauccaac aguuugauua ccagaaaaag     3000

cuggacaauc gagaaaaaga gcggguagca gcgcgucagg ccuggagugu uguggggaca     3060

auuaaggauc uuaagcaagg auaucuaagc caggucaucc acgaaauugu ggaccucaug     3120

auccacuacc aggcugucgu gguucuggag aacuugaauu ucgguuuuaa aucuaagaga     3180

accggcauag ccgaaaaagc aguguaccag caauucgaga agaugcugau cgauaaauua     3240

aacugccucg uacugaagga cuauccugcu gagaaggucg ggggagugcu uaauccauac     3300

cagcugacug aucaguuuac auccuucgcc aaaaugggca cccaaucagg uuucuuguuu     3360

uauguucccg caccguacac aaguaagauu gacccucuca ccggguucgu ggauccauuu     3420

gucuggaaaa caaucaagaa ccaugaaagc aggaaacacu uccuggaggg cuuugacuuc     3480

cuacauuaug augugaagac gggagacuuu auucugcacu ucaagaugaa ucgcaaccuu     3540

ucguuucagc ggggccuccc cggguucaug ccugccuggg acaucguauu ugagaaaaau     3600

gaaacccagu ucgaugcuaa gggaacuccc uuuauagcgg guaaaagaau ugugccaguu     3660

aucgagaacc auagguucac aggccgauac cgcgaccugu auccugccaa ugaauugauc     3720

gcacuguuag aggaaaaggg gauugucuuc cgggauggau ccaacauacu ccccaaacug     3780

cuugagaaug acgauucuca cgcuaucgac accauggugg cccugauuag aagcguguug     3840

cagaugagga acucaaaugc cgcuacaggc gaagauuaca ucaacagucc aguccgugac     3900

cucaacggug uuuguuuuga uucccgcuuc caaaauccgg aguggccuau ggacgcagau     3960

gccaacgggg cguaccacau ugcucugaag ggccagcuac ugcuuaauca ucucaaagag     4020

ucuaaggacc ugaaguugca gaacggaauc agcaaucagg acugguuagc cuauauacaa     4080

gaacugcgga acaaaagacc cgcagccacu aagaaagcug gccaggcaaa gaaaaagaag     4140

uaaggacuag ucccuguucc cagagcccac uuuuuuuucu uuuuuugaaa uaaaauagcc     4200

ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaga auu                                             4283


<210>  3665
<211>  4262
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3665
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcaagcauu      180

uaccaggagu ucguuaacaa guauucccug ucuaaaaccc ucagguuuga acuuauacca      240

caaggaaaga cacuggagaa uaucaaggcc cgggguuuga uuuuagacga ugaaaaacgc      300

gcgaaggacu acaaaaaggc uaaacagauc auugauaagu aucaucaguu cuuuaucgag      360

gaaauacugu caagugugug caucagcgag gaccuacucc agaacuacuc cgauguauau      420

uucaagcuga aaaagucuga cgaugacaau cuucaaaaag auuuuaaguc ggccaaagac      480

acuauuaaga aacagaucuc agaguacauu aaggauagcg aaaaguucaa aaaccuguuu      540

aaucagaacu ugaucgacgc aaagaaaggc caggagagug aucucauacu guggcugaag      600

caauccaaag acaaugggau ugaacucuuc aaggccaacu cugacaucac ggauauugac      660

gaggcuuuag agaucaucaa gagcuuuaaa ggauggacca cauauuucaa gggcuuucac      720

gaaaaucgua aaaacgugua cuccucaaau gauauuccga caaguauaau cuaccgaauu      780

gucgacgaua accugcccaa guuccuugag aauaaagcaa aguaugagag cuugaaggac      840

aaagccccug aggcuaucaa cuacgaacag auaaagaaag aucuggccga ggagcuaacc      900

uuugacauug auuauaagac aucugaagug aaucagagag uuuucucccu cgacgaggug      960

uucgaaaucg cgaacuuuaa caauuaccug aaccagucag guaucaccaa auucaauacu     1020

auuaucgggg gaaaguuugu caacggcgag aauacaaaga ggaaaggcau uaacgaauau     1080

auaaaucuuu acagccaaca gaucaacgau aagacccuga aaaaguauaa aaugagugug     1140

uuguucaagc agauucucuc ugacacggag uccaagagcu uuguaaucga caaacuggag     1200

gaugacucgg auguugugac uaccaugcaa ucuuucuacg aacagaucgc agccuuuaag     1260

acagucgagg aaaaauccau uaaggagacu uuaucacucc uguucgacga ucuuaaagcu     1320

cagaagcugg acuugaguaa aauauacuuu aagaaugaua agagccuaac cgaccugucc     1380

cagcaagugu ucgaugacua uucugugauc gggacagccg uccucgaaua cauuacccag     1440

cagaucgcac ccaaaaaccu ggauaaucca agcaagaaag agcaggagcu uauugcuaag     1500

aaaacggaaa aggccaagua ucugucauug gagacuauca aacucgcgcu ggaagaguuu     1560

aacaagcauc gggacauaga uaaacaaugu cgcuucgagg aaaucuuagc caauuuugca     1620

gcuauuccua ugaucuucga cgagauugcc cagaacaagg acaaucuggc ucagaucagu     1680

auaaaauacc agaaccaagg aaagaaggau cuacuccagg ccuccgcaga agacgauguu     1740

aaagccauua aggaccugcu ugaucagaca aacaauuugc ugcacaaacu caagaucuuc     1800

cauauuagcc agucugagga caaagcuaac auccuggaua aggacgaaca cuuuuaucuu     1860

guguucgagg agugcuacuu ugaacuggcg aauauaguac cauuguauaa caaaaucaga     1920

aauuacauua cccaaaagcc cuacucagau gagaaguuca aacucaacuu ugaaaauagu     1980

acucuggcaa acgguuggga caagaauaaa gagccugaua acacagccau cuuauucauu     2040

aaggacgaua aauauuaccu gggcgugaug aauaagaaga acaauaaaau cuuugacgac     2100

aaggcuauaa aagaaaacaa gggggaggga uauaaaaaga uugucuacaa gcuacuccca     2160

ggcgccaaua aaaugcugcc gaagguguuc uuuagcgcaa aauccaucaa guucuauaac     2220

cccucugagg auauccuuag gauucggaac cacucgaccc auacgaaaaa ugguagcccu     2280

cagaaggggu acgaaaaguu ugaguucaac aucgaagacu gucgcaaauu uauugauuuc     2340

uauaagcagu ccauaucaaa acacccagag uggaaggacu ucggauuucg auucagugau     2400

acccaaagau acaauucuau cgacgaauuu uacagggagg uugagaacca gggcuauaaa     2460

uugacauucg aaaauauuag cgaguccuac aucgauucag uggucaacca gggcaagcug     2520

uaucucuuuc agauuuacaa uaaggacuuc agcgccuauu cuaaagggcg gcccaaccug     2580

cauacuuuau acuggaaggc ucuguuugau gaacguaauc uucaagacgu gguauauaaa     2640

uugaacggag aggccgaacu cuucuaccgc aagcagucca ucccuaaaaa gauaacccac     2700

ccagcaaaag aagcgaucgc uaauaagaac aaggauaauc ccaaaaagga gaguguuuuu     2760

gaauacgacc ugauuaaaga caagagauuc acagaggaua aauuuuucuu ccacugcccg     2820

aucacuauua acuuuaagag cucuggugcc aauaaguuca acgacgaaau caaccuacug     2880

cucaaagaga aggcaaauga ugugcauaua cuguccauug acaggggcga gcggcaccuu     2940

gccuauuaca ccuuggucga ugggaaagga aacaucauca agcaggacac auuuaauauu     3000

auaggcaacg aucgaaugaa aaccaauuau caugacaagc uggcugccau cgaaaaggau     3060

cgcgacucag caagaaaaga uuggaagaaa auuaacaaua ucaaggagau gaaagaaggu     3120

uacuuaaguc agguggucca cgagauugcu aagcucguga ucgaauauaa cgccauaguu     3180

guguucgagg accugaauuu uggguucaag aggggccggu uuaaaguaga gaagcaaguc     3240

uaccagaaac uugaaaagau gcugaucgag aaauugaacu aucucguguu caaggauaau     3300

gaauuugaca aaacuggagg cguucugcgc gcguaccagc uaacggcccc uuucgagaca     3360

uuuaagaaga uggggaaaca gaccggaauu aucuacuaug ugcccgcugg uuucacuagc     3420

aagauuuguc cagucaccgg cuuugugaac caacuguacc cuaaauauga aucgguaucc     3480

aagucucagg aguucuucag caaauuugac aagaucugcu acaaucucga uaagggguau     3540

uucgaguuuu cauucgacua caaaaacuuu ggagauaagg cagccaaagg caaguggaca     3600

auagcuaguu ucgguuccag acugauuaau uuuaggaacu cugacaaaaa ucauaacugg     3660

gauacccgug aaguguaucc cacuaaggag cuugaaaagu ugcugaaaga cuacagcauc     3720

gaguacgggc acggcgagug uaucaaggcc gcaauuugcg gagaauccga uaaaaaguuc     3780

uuugcgaaau uaacaucagu ucucaacacg auccugcaga ugcggaauag caagaccggc     3840

acugagcuug acuaucugau aaguccaguc gccgauguga acgggaauuu cuuugacucu     3900

cgacaagcuc ccaagaacau gccucaggau gccgacgcaa auggagcuua ccacauuggu     3960

cuaaaaggcc ucaugcuguu ggggagaauc aagaacaauc aggaaggcaa aaagcugaac     4020

uuagugauua aaaaugagga auauuucgag uuuguccaga acaggaauaa caagcgccca     4080

gccgcaacua aaaaggccgg acaagcuaag aaaaagaaau aaggacuagu cccuguuccc     4140

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     4260

uu                                                                    4262


<210>  3666
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3666
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcagac aagaaauaca gcauuggacu ggauauaggu      180

acaaacuccg uuggcugggc cgugaucaca gacgaguaua agguaccauc uaagaaauuc      240

aaggugcucg ggaauacuga uaggcauuca auuaaaaaga accuuaucgg agcgcuguug      300

uuugacagug gcgaaacggc ugaggccacc cgguuaaaac gcacagcacg acguagauac      360

acuaggcgga agaaucgcau uugcuaucug caggaaaucu ucagcaacga gauggccaag      420

gucgaugacu ccuuuuucca cagacuagaa gagucuuuuc ucguggagga agauaaaaag      480

caugagaggc acccgauauu cgguaauauc guugacgaag uggcuuacca ugagaaauau      540

cccaccauuu accaucugcg gaagaaacuu gucgauucga cagacaaggc agaucugcgc      600

uugaucuauc ucgcccuggc ucacaugauu aaauuucgag ggcacuuccu gaucgaggga      660

gaccucaacc cugauaauuc agacguggac aaguuauuua uacaacuggu acagaccuac      720

aaccagcuuu ucgaagagaa uccaauuaac gccagcggcg uugaugcgaa ggcaaucuug      780

agugccagac uguccaaauc uaggcggcua gaaaaucuca uugcucagcu gcccggcgag      840

aagaaaaacg ggcuuuuugg aaaucugauc gccuugagcc ucggucugac uccuaacuuc      900

aaguccaauu uugacuuagc agaagaugcu aaacugcaac ucucaaagga cacauacgau      960

gacgaucuug acaaccuguu ggcccagauc ggcgaucagu augcggaccu auuccuggcc     1020

gcaaagaacc ucagugaugc uauucugcuu agcgacauac ugcgugugaa uaccgagauc     1080

acgaaagccc cauugucugc uuccaugauu aagcgcuacg acgagcacca ucaggaucuc     1140

acucuguuaa aagcccuggu cagacaacag cuacccgaaa aguauaaaga gaucuucuuu     1200

gaccagucaa agaacgggua cgcaggauau auagauggcg gugccagcca ggaagaguuc     1260

uacaaguuua uuaaaccuau ccucgaaaag auggacggga ccgaggagcu gcuugugaaa     1320

uugaauaggg aagaucugcu ccggaagcaa cgaacauucg acaacggaag uaucccacac     1380

cagauucacc ugggcgagcu ucaugcuauc cugcgcagac aggaagauuu uuauccguuc     1440

uugaaagaca auagggagaa gauugaaaag auacucacuu uucggauccc cuacuacgug     1500

ggcccucugg cgcgcgggaa cucuagauuc gcauggauga ccaggaaauc cgaggagaca     1560

auuaccccau ggaauuuuga agaggucguu gauaagggag ccagcgcuca aucguucauc     1620

gaacguauga cgaacuuuga caaaaauuua cccaacgaga aggugcugcc uaaacacucu     1680

cuacucuaug aguacuucac uguauauaau gaacugacaa aggugaaaua cgucaccgag     1740

gguaugcgga agccagccuu ucuuuccggc gaacagaaga aagcaaucgu ggauuugcug     1800

uucaagacua accgaaaagu uacagugaag cagcucaaag aggacuauuu caagaagauu     1860

gaauguuuug auucagucga gauaaguggg guggaggaca gauucaaugc cagccuggga     1920

accuaccaug acuuacugaa aaucauuaag gauaaagacu uucuugauaa cgaagagaau     1980

gaagacaucu uggaggauau uguacucacg cugacccuau ucgaagacag ggagaugauc     2040

gaggaacgcc ugaagacaua ugcucaccuc uuugaugaca aaguuaugaa gcagcugaag     2100

cggagaaggu acacuggcug gggucgccuu ucccgaaaau ugauaaacgg gauccgggau     2160

aagcaaucug gcaaaaccau ucuggacuuc uuaaagagcg auggauuugc caaccguaau     2220

uucaugcagc ucauccauga cgauucacug acauuuaaag aggacauuca gaaggcacag     2280

gugaguggcc aaggggacuc ccuucacgaa cacaucgcga accuggcugg aagccccgcc     2340

auaaagaaag guauuuugca gacugucaag guggucgaug agcucgugaa aguuaugggc     2400

agacauaagc cggaaaauau cgugauugag auggcaaggg agaaccagac cacacagaaa     2460

gggcaaaaga acucucgcga acggaugaaa agaaucgagg aaggaauaaa ggagcugggc     2520

ucacagaucc uaaaggagca cccuguagaa aauacccagc ugcagaauga gaaacucuac     2580

cuguauuacc uucaaaacgg uagggacaug uaugucgauc aggaauugga cauuaaucga     2640

cugagugauu acgacgugga ucauaucguu ccccagagcu ucuuaaagga cgauuccauu     2700

gacaacaaag ugcucacucg cucugauaag aaucggggga aaucggacaa cgucccaagc     2760

gaggaagugg uaaagaagau gaaaaauuau uggagacaac ugcuuaacgc caagcugauc     2820

acgcagagga aauuugacaa ucuaacaaag gcugagcgug gcggacucuc cgagcuggau     2880

aaagccggcu ucauaaagcg ccaguuggug gaaacccggc agauuacuaa gcacguugca     2940

caaauccugg acucaagaau gaacaccaaa uacgaugaga acgacaaguu aaucagggaa     3000

gucaaaguga uuacacucaa gaguaaacug gugucugauu uucgaaagga cuuccaguuc     3060

uauaaggucc gggagaucaa uaacuaccac caugcucacg augccuaccu uaaugcgguu     3120

guggggaccg cccugauuaa aaaguauccu aaauuggaaa gcgaguuugu guacggagac     3180

uauaagguau acgauguccg caaaaugaua gcuaaguccg agcaggaaau cgguaaggca     3240

acugccaaau auuucuuuua cucaaacauu augaauuucu uuaagacaga gaucacgcuc     3300

gcuaacggcg aaauuagaaa aaggccccug aucgagacca auggggaaac uggagagaua     3360

gugugggaca agggccggga uuucgccaca guucgaaaag ugcuaagcau gccacagguc     3420

aacaucguga agaaaaccga gguucaaaca gguggguuuu cuaaggaauc cauucugccc     3480

aagagaaaua gugacaaacu uaucgcacgc aagaaagauu gggacccuaa gaaauauggc     3540

ggauucgaca gcccaacugu ggcguacucu guacucgucg uggccaaggu ggagaagggc     3600

aaauccaaga aacugaaguc agucaaagaa uugcugggga uuaccaucau ggagaggagu     3660

agcuuugaga agaacccgau agauuucuua gaagcuaagg gauacaaaga gguuaagaaa     3720

gaccucauua ucaagcugcc caaauauucg cuuuuugaac uggagaaugg ccguaagcgg     3780

auguuggccu ccgcugguga acuccagaag gggaacgagc uggcacuacc uucuaaauac     3840

gugaauuucc uguaucuugc cagccauuac gagaagcuca aaggaucacc agaagauaac     3900

gagcagaagc agcuguucgu agaacaacac aaacauuauu uggacgagau cauugaacag     3960

auaagugagu uuuccaagag agugaucuua gcagaugcca aucuggacaa aguccucucu     4020

gcuuacaaca agcacaggga uaagcccauu cgcgagcagg cggaaaacau cauucaccug     4080

uucacgcuua ccaaucuggg cgccccugca gcuuuuaaau auuucgacac aacuaucgau     4140

cgaaagagau acaccagcac aaaagaggug uuggacgcca cucuaauaca ucaauccauc     4200

accggucugu acgaaacgcg gauugaucuc ucacagcugg gcggggacaa gaggccagcc     4260

gcuaccaaaa aggcaggaca ggccaagaaa aagaaauaag gacuaguguc caccuguccc     4320

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     4380

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaagaa uu                                                         4452


<210>  3667
<211>  5235
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3667
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaac uucaagauuc ugccaauagc caucgaccuc      180

ggaguuaaaa auaccggugu guuuagcgcg uucuaccaga agggcacauc ccuugagagg      240

cuggauaaca agaaugggaa aguauaugaa uugucuaagg acucauacac uuuacugaug      300

aacaaucgga cggcucgccg acaucaacgu agaggaauug auaggaaaca gcuagugaag      360

cggcucuuua aacugaucug gaccgagcag cuuaaccugg aaugggacaa ggauacacag      420

caagccauua guuucuuguu uaaucgcaga ggcuucagcu uuaucacuga cgguuauucc      480

ccggaguacc ucaacauagu ccccgaacag gugaaggcaa uccugaugga uauuuucgac      540

gauuauaaug gggaggacga ucuggacucu uaccucaaau uagccaccga gcaggaaucg      600

aagaucucag agauuuauaa caaacugaug cagaagaucc uugaauuuaa auugaugaag      660

cugugcacag auauaaaaga cgacaagguu agcaccaaga cucuaaaaga gauuacaagu      720

uacgaguucg aacuccuggc ugauuaccuu gcaaauuauu ccgagucucu gaagacccaa      780

aaauuuagcu acacggacaa gcagggaaac uugaaagaac ucuccuauua ccaccaugau      840

aaguauaaua uccaggaguu ccugaagagg cacgccacua uuaacgaccg gaucuuagau      900

acccugcuca cagacgaucu ugacaucugg aauuuuaacu ucgaaaaauu cgauuuugac      960

aagaacgagg agaaacugca gaaucaagaa gauaaggacc auauucaggc ucacuugcac     1020

cauuucgugu uugccgucaa caaaauaaag ucagagaugg cgaguggcgg ccgccaccga     1080

agccaguacu uccaagaaau cacuaaugug cuagacgaga acaaucauca ggaaggguau     1140

cugaagaacu uuugugagaa ucuccacaac aaaaaguacu cuaaucuguc cguuaaaaac     1200

cuuguaaauc ugauuggaaa cuugucaaau cucgagcuga agccuuuaag aaaauacuuc     1260

aacgauaaga uccacgcaaa ggccgaccau ugggaugaac agaaauuuac cgagacauau     1320

ugccacugga uacuggguga auggagggug ggcgucaagg accaggauaa aaaggacggg     1380

gcuaaauaca gcuauaagga ucuauguaau gagcucaaac aaaaggugac caaggccgga     1440

cugguggacu uccuuuugga acuggaucca ugccggacga uucccccuua ccucgacaac     1500

aacaaucgua aaccacccaa gugucagagu cugauccuua acccuaaauu ucuggauaau     1560

caguauccaa acuggcagca auacuugcag gagcucaaga aacugcaguc uauccagaau     1620

uauuuagacu ccuucgagac ugaucugaag guccuaaaga gcucgaaaga ccaaccguac     1680

uuuguugaau acaagucuuc caaccagcag auugcaucag gccagcgcga cuauaaagau     1740

cucgacgcua gaauccugca auucauuuuu gauaggguga aggccaguga cgagcuuuug     1800

cugaaugaaa uauacuucca ggcgaaaaag cucaagcaga aagccagcuc cgagcuggag     1860

aaguuagaau cuagcaaaaa gcuggaugag guaaucgcaa acucacaacu uagucagauu     1920

uugaaauccc agcauacaaa ugguaucuuc gaacagggga ccuuucucca ccuggugugc     1980

aaguauuaca agcaacggca gcgagcucgc gacagcagac uauauaucau gcccgaguac     2040

agguaugaua aaaagcugca uaaauacaac aauacuggac gguucgacga ugacaaccag     2100

cuccugacau acuguaauca caagccucgc cagaaaagau aucaacuuuu gaacgaucug     2160

gccggcgucu uacagguguc uccaaauuuu cucaaggaca aaauuggcuc agaugacgac     2220

cuguucauaa guaaguggcu uguugaacac aucagggggu uuaagaaagc augcgaggau     2280

agccugaaga uucagaaaga caaccgugga uugcucaacc auaagaucaa uauugcccgg     2340

aacaccaaag guaaguguga gaaggaaauc uucaaucuga uaugcaaaau cgagggcucc     2400

gaagauaaga aagggaacua caagcacgga cuagcauaug agcugggcgu gcuccuguuu     2460

ggugaaccca augaggccuc uaaaccugag uucgaccgaa agauuaagaa auuuaacucg     2520

aucuacagcu ucgcucagau ucaacagauc gcguuugcag aaagaaaggg gaaugccaac     2580

acgugugcug ucugcuccgc cgauaaugca cauaggaugc agcaaauaaa aauuaccgag     2640

ccaguggaag acaacaagga uaaaaucauu cuuucagcca aggcucagcg cuugcccgcc     2700

aucccgacac ggauaguaga cggcgcaguu aagaaaaugg cgacuauccu ggcuaagaau     2760

auuguggaug acaacuggca gaauaucaaa caggucuuaa gugccaagca ccaacuccac     2820

auuccuauca uaaccgaguc uaacgcauuc gaauuugagc ccgcccuggc ugaugugaaa     2880

ggaaagagcc uuaaggacag aaggaaaaag gcccuggagc gcauuucccc agaaaauauc     2940

uucaaagaca agaacaaccg aaucaaagag uucgcaaagg gcauuucagc uuauagcggg     3000

gccaaucuaa cagauggaga cuuugauggu gcgaaagaag aacucgacca uaucauuccu     3060

cggucucaca agaaguacgg cacucugaac gaugaggcca auuugauaug cgucacccgu     3120

ggggacaaca aaaauaaggg aaacagaauc uucugucuga gggauuuagc ugacaauuau     3180

aaacucaagc aguuugaaac aaccgaugac cuggagauug agaaaaagau cgcagauacu     3240

auuugggacg ccaacaagaa agauuucaag uuuggcaauu accgcuccuu caucaaccuu     3300

acgccccagg agcagaaggc uuuucggcau gcccuguucu uggcagacga aaauccaaua     3360

aaacaagcgg ugaucagagc cauuaacaau aggaaccgaa cauuuguuaa ugguacccag     3420

cgcuacuucg cugaggugcu cgccaacaau aucuaucugc gggcuaaaaa ggagaaccua     3480

aacacugaca agauuaguuu ugauuacuuc gggaucccca ccauaggcaa uggaagaggc     3540

auugcugaaa ucaggcagcu guaugagaaa guagacagcg auauccaggc cuacgcaaag     3600

ggggacaaac cucaagccuc uuauucccac cuuauugaug cuaugcucgc guucugcaua     3660

gccgcagacg aacaucguaa cgauggauca aucggucugg agauugacaa gaauuacagu     3720

uuguauccac uggauaaaaa cacaggcgaa gucuuuacca aggacaucuu cagccagauu     3780

aagaucacug auaaugaguu uucggacaaa aaguuagugc gcaaaaaggc uauagagggg     3840

uucaacacac accggcagau gacgagagau ggaaucuacg ccgaaaauua ccucccgauu     3900

cugauccaca aagagcuuaa cgaaguuagg aagggcuaua ccuggaaaaa uuccgaggaa     3960

auuaagaucu uuaaggguaa aaaguacgac auacagcaac ugaacaauuu gguguauugu     4020

cucaaauucg ucgacaagcc cauuucuauc gauauccaga uuagcacucu ggaggagcua     4080

cgaaacaucc ugacaaccaa uaacauagcc gcuacagcag aauacuauua cauuaaucuu     4140

aaaacucaga agcuccauga guauuacauc gaaaacuaca acaccgcccu gggguauaag     4200

aaauacucaa aggagaugga guuuuugcgg aguuuagcgu aucgcuccga aagagugaaa     4260

auuaagucua ucgacgaugu aaaacaagug cuggacaagg auagcaauuu cauaauuggc     4320

aagaucacgc ucccuuuuaa aaaggagugg cagaggcugu accgggaaug gcagaacacc     4380

acaaucaaag acgauuauga guuccuuaag uccuuuuuca auguuaaauc aauuacuaag     4440

cugcacaaga aaguccgaaa ggacuuuagc uugccaauca guaccaacga aggaaaauuc     4500

cuagugaaga gaaaaacaug ggauaauaac uucauuuacc agauacugaa ugacucugau     4560

ucccgcgcug acggcacuaa gcccuuuauc ccugccuucg acauuagcaa aaacgagauc     4620

guggaggcaa ucauugauuc guuuaccuca aagaauauau ucuggcuccc aaagaacauc     4680

gaacugcaaa aagucgacaa uaagaacauu uuugccaucg auacgucuaa augguucgag     4740

guugaaacac ccagugaccu uagggauauu gggaucgcua ccauacagua uaagaucgac     4800

aauaacuccc guccgaaagu gcgggugaag uuagauuacg uaauugacga ugacagcaag     4860

aucaauuacu uuaugaacca uucucugcuc aaaagcagau auccugauaa ggucuuggag     4920

auucugaaac aguccacuau cauagaguuc gaaucaagug gauuuaacaa gacaauuaaa     4980

gagaugcucg gcaugaagcu ggcagguauc uacaaugaaa ccagcaacaa uaagaggcca     5040

gccgcgacua aaaaggcugg gcaggccaaa aagaaaaagu aaggacuagu guccaccugu     5100

cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac     5160

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5220

aaaaaaaaaa gaauu                                                      5235


<210>  3668
<211>  3507
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3668
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaag aggaacuaca uucugggacu cgacauaggu      180

aucacaucag uuggcuaugg gauuaucgau uacgagacac gggacgugau ugaugccgga      240

guacgccuuu ucaaagaagc gaauguggag aacaaugaag gccgacguuc caagagaggu      300

gcuaggcggc ugaagcgcag aaggcggcau cgcauccagc gagucaaaaa guuguuauuu      360

gacuauaacc ugcuaacuga ucacucugag cucucaggga uaaauccaua cgaagccaga      420

gugaaaggac ugagucaaaa acuuagcgag gaggaguucu ccgcagcccu guugcaucuc      480

gcuaaaaggc ggggcguuca caacgugaau gaagucgaag aggacacggg caacgagcug      540

ucuaccaagg aacagaucuc gcguaauuca aaggcacugg aggaaaaaua uguggccgag      600

cuccaguuag aacgccugaa gaaagauggg gagguaagag gaagcauuaa cagguuuaag      660

acaagugacu acguuaaaga ggcuaagcag cuuuugaaag ugcaaaaggc cuaucaucag      720

cuggaucagu ccuucaucga cacuuacauu gaucuacucg aaacccggcg aacauacuau      780

gaggguccgg gcgaaggguc ucccuuugga uggaaggaca ucaaagagug guacgaaaug      840

cugaugggcc acugcaccua uuucccugag gagcuucgca gcgucaagua cgcguauaau      900

gcagaucugu acaacgccuu gaaugaccuc aacaaucugg ugauaacuag agacgaaaac      960

gagaaauuag aauauuacga gaaguuucag auuaucgaaa acguguucaa acaaaagaag     1020

aaaccaacac ugaagcagau ugcuaaagag auccucguca augaggaaga uaucaagggu     1080

uacaggguua ccuccacggg gaaacccgag uuuacuaacc uuaaggugua ucacgacauu     1140

aaggauauaa ccgcccggaa agaaaucauu gagaaugcag agcuguugga ccagaucgcu     1200

aagauacuaa caauuuacca gucaagugaa gauauccaag aggaacugac uaaccucaau     1260

agcgagcuga cccaggaaga gaucgagcag auuucuaacc uuaaaggaua uacaggcacc     1320

cauaaucugu ccuugaaggc caucaaccuc auucuggacg aauuauggca cacgaaugau     1380

aaccaaauag cgaucuucaa ucgccugaaa cuuguaccua agaaggugga ccucucacag     1440

cagaaagaga uuccaacuac acuggucgau gacuuuaucc uuagccccgu gguuaagaga     1500

aguuucaucc agucuauuaa agugauaaac gccaucauua agaaauacgg cuugccuaau     1560

gauaucauua ucgaacuggc aagggagaag aacuccaaag acgcucaaaa gaugauaaau     1620

gaaaugcaga agcguaaccg gcagaccaac gagcgaaucg aggaaauuau cagaacuaca     1680

gggaaagaga augccaagua ucucauugaa aagaucaagc ugcaugacau gcaggaggga     1740

aaaugucuuu acagccugga agcuauacca uuggaggauc uccugaacaa uccguuuaac     1800

uaugaggucg accacauuau ccccaggucg gugucuuucg auaauuccuu caacaauaag     1860

guauuaguua agcaagaaga gaacucaaag aaggguaauc gcaccccuuu ucaguaccug     1920

aguagcuccg acucuaaaau uagcuacgaa acguucaaga aacacauccu aaaccucgcc     1980

aagggcaagg ggcggauauc aaaaaccaag aaagaguauc ugcuugagga aagagauauc     2040

aauagguuua gugugcagaa ggacuucauu aaccgcaauu uggucgauac acgauacgca     2100

acucggggac ugaugaaccu ccugcguucc uauuuuagag ugaauaacuu agacgucaaa     2160

gugaaaagca ucaacggcgg uuucaccucu uuucugaggc gcaaguggaa auucaagaaa     2220

gagcggaaua agggguacaa acaucacgcc gaagaugcuc uuauuaucgc gaacgcagac     2280

uuuauauuca aggaguggaa aaaguuggau aaggccaaaa agguuaugga aaaucagaug     2340

uuugaggaga aacaagcuga aucaaugcca gagauugaaa cagagcagga auauaaggag     2400

aucuucauca cuccccauca gauuaaacac aucaaggacu uuaaggauua caaauauagu     2460

cauagagugg acaagaaacc uaacagggag cucauuaaug acacccugua cagcacacga     2520

aaggaugaca aaggcaacac ccuaauagua aauaaccuga auggacucua cgauaaggac     2580

aacgauaagc ugaaaaagcu aaucaauaaa uccccagaaa aguugcugau guaucaccac     2640

gacccccaga cuuaccaaaa auuaaagcuc auuauggagc aguauggcga ugaaaagaac     2700

ccgcuguaca aauauuacga ggaaacgggg aauuaucuua caaaguacuc uaaaaaggac     2760

aacggaccug ucaucaaaaa gauuaaguac uaugguaaua aacugaacgc ccauuuggau     2820

aucaccgacg auuaccccaa cucgcgcaau aaggugguua aacucagccu gaagccauau     2880

cgguucgacg uguaccuaga uaacggcguc uauaaauucg ugacuguaaa gaaucuggac     2940

gugaucaaaa aggagaacua cuaugagguu aauuccaagu gcuacgaaga ggccaaaaag     3000

cucaaaaaga uaucaaacca ggcagaauuu auugcuaguu ucuacaauaa cgaccugauc     3060

aaaauuaaug gggagcuuua uagagucauc ggagugaaca augauuugcu gaacaggaua     3120

gaggugaaua ugauugacau caccuaccgu gaauauuuag agaacaugaa ugauaagcgc     3180

ccuccccgga ucauuaagac aauagccucu aaaacccaaa gcaucaagaa auacuccacu     3240

gacauucucg gcaaccugua ugaagucaag agcaaaaagc acccacagau cauuaagaaa     3300

gguaagagac ccgcagcgac caaaaaggcu gggcaggcca aaaagaagaa auaaggacua     3360

guguccaccu gucccuccug ggcugcugga uugucucguu uuccugccaa auaaacagga     3420

ucagcgcuuu acagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aagaauu                                         3507


<210>  3669
<211>  4575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3669
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcacug uucaacaagu gcauuauaau cagcauuaau      180

cucgacuuuu ccaacaaaga gaaguguaug accaagccau acucuaucgg acuugauauu      240

gguacaaauu caguuggcug ggccgugauc acugacaacu auaaaguacc gaguaagaaa      300

augaaggugc uggggaauac gagcaaaaag uacauaaaga aaaacuuguu aggaguccug      360

cuauucgauu ccggcaucac cgcggaaggu aggcggcuca agcgcacagc ucgacguaga      420

uauacuaggc ggcgcaauag gauucuguac cuucaggaga ucuuuucuac cgaaauggcc      480

acacuggacg augcauucuu ucaaagauug gacgauucgu uccucgugcc cgacgauaaa      540

cgggacucaa aguauccuau uuuugggaac cugguugagg aaaaagugua ccaugaugag      600

uucccaacca ucuaucaucu gcgcaaguac cucgccgaca gcacuaaaaa ggcugauuua      660

cgacuggucu accuugcauu ggcccacaug auaaaguaua gaggacacuu ucugauugag      720

ggcgaguuca auaguaaaaa caaugacauc cagaagaacu uucaggacuu ccuagauaca      780

uacaaugcua uuuuugaguc cgaccucucu cuggaaaaca gcaaacagcu ugaggagauc      840

gugaaggaua aaaucuccaa gcuggaaaag aaagacagga uuuugaaacu cuuccccggc      900

gagaaaaauu cagggauauu cagugaauuu cugaaguuaa ucguaggaaa ccaagccgau      960

uuccggaaau gcuuuaaccu ggacgagaag gcgagccucc auuucucuaa ggaauccuau     1020

gaugaggacc uugagacccu guuggguuac auuggcgaug acuauucaga uguuuuucua     1080

aaagcaaaga aacuguacga cgccauccuc cugagcgggu uccuuacggu gacugacaau     1140

gaaaccgagg cuccucugag uucugccaug auaaagcguu auaacgaaca caaagaggau     1200

uuggcacucc ugaaggaaua cauucgcaau aucuccuuaa agacauacaa cgaggucuuu     1260

aaagacgaua cuaagaaugg auaugcuggc uacaucgacg guaaaaccaa ccaggaggau     1320

uucuaugugu accugaagaa ucuacucgcc gaauuugagg gggcggacua uuuccuggaa     1380

aagauugaua gagaggacuu ucuuaggaag cagcggacau ucgauaacgg aagcauccca     1440

uaccagauuc acuugcaaga aaugcgagcc auacuggaca aacaggcaaa guuuuauccc     1500

uuccucgcua agaauaaaga gcgcaucgag aagauucuga ccuucagaau cccuuacuac     1560

gugggcccac uugccagggg caacucggau uuugcuuggu cuauccggaa acgcaaugaa     1620

aagauuacgc cguggaacuu cgaggacguc auagauaaag aauccucagc cgaggcauuu     1680

aucaauagaa ugacaaguuu cgaccuguau uugcccgagg aaaagguucu cccuaagcau     1740

agccuguuau acgagacuuu uaacguguau aaugaacuga ccaaaguaag guucauugcc     1800

gaguccaugc gugacuacca guuucuagau ucuaagcaga aaaaggacau cgugcggcuc     1860

uauuucaaag auaagcgaaa ggucacugac aaagauauua ucgaauaccu gcacgcuaua     1920

uauggguacg acggaaucga gcuuaagggu auugagaaac aauuuaacag cucauugagu     1980

acauaccaug aucugcucaa caucauuaau gacaaggagu uccuggauga cuccagcaac     2040

gaagcgauca uagaagagau uauccacacc uuaacgauuu uugaagauag agagaugauc     2100

aaacagaggc ugucuaaguu cgagaauaua uuugacaagu cagugcuuaa aaaguugagu     2160

cgccggcacu auaccggcug ggggaaacuc agcgcaaagc ugaucaacgg aauuagagau     2220

gaaaaauccg gcaauacaau ccuagacuac cugauugacg augguaucuc uaacaggaau     2280

uucaugcagc ucauacauga cgaugcccug ucguucaaga aaaagauuca aaaggcucag     2340

aucaucgggg acgaggauaa aggcaacauu aaggaaguug ugaaaagccu uccaggaucc     2400

cccgccauca agaaaggcau uuugcaguca auaaagaucg ucgacgagcu ggugaaggua     2460

augggagggc gcaaaccuga aaguauuguu guggagaugg cacgagagaa ucaguauacu     2520

aaccaaggua agucuaauag ccagcagcgg uuaaaacguc ucgaaaaguc ccugaaagag     2580

cuuggcucaa agauccugaa ggaaaacauu ccagccaaau ugagcaagau cgauaauaac     2640

gcucuccaga augacagacu guaccuauau uaccugcaaa acgggaaaga uauguauacc     2700

ggagacgauc ucgacauaga caggcugucu aacuacgaua ucgaccacau uaucccccag     2760

gccuuucuua aggauaauuc cauugacaac aaagucuugg ugaguagcgc aucuaaucgc     2820

ggcaaguccg augacuuccc gucacuggag gucgugaaga aacggaagac auuuugguac     2880

caguuacuca aaaguaagcu gaucagccag agaaaauucg auaaccuuac uaaggcggag     2940

aggggugggc ugcuaccuga agacaaggcu ggcuuuauac aacgacagcu cguugagacc     3000

cgccagauua caaaacaugu ggcccggcug uuggaugaaa aguucaauaa caaaaaggac     3060

gagaauaaca gagcaguaag gaccgucaaa aucaucacuc ugaagucgac guuagugucc     3120

caguuucgua aagauuucga acucuauaag guucgcgaga uuaaugacuu ucaccaugcc     3180

cacgaugcuu accugaacgc cgugauagca ucugcccuuc ugaagaaaua ucccaaguug     3240

gagccagagu ucgucuacgg agacuauccu aaauacaaua gcuuucggga aagaaaguca     3300

gcuacagaaa aaguguauuu cuacaguaac aucaugaaua uuuucaagaa guccaucucu     3360

cucgcggacg gcaggguaau ugagcgaccc cugaucgaag ugaacgagga gaccggggaa     3420

agcguuugga auaaagaguc cgaucuagcc acuguccggc gcgugcuguc auacccacaa     3480

gugaacgucg uuaagaaagu ggaagaacag aaccacggac uugacagagg uaagcccaaa     3540

ggccucuuua augcuaaccu gagcaguaag ccuaagccaa auucuaacga aaauuuggug     3600

ggggcaaaag aguaucugga uccgaagaaa uacggaggcu augccgguau auccaacagc     3660

uucgcuguau uagucaaggg gaccaucgag aaaggcgcca agaagaaaau uacaaaugug     3720

cucgaauuuc agggaaucuc gauucuggac aggaucaacu accggaagga uaaacuuaau     3780

uuccuguugg agaagggcua uaaagacaua gaacucauua ucgagcugcc caaguacuca     3840

cuauuugagc ugucugaugg gagucgaaga augcuugcau ccauccucag caccaacaau     3900

aaacgcggag aaauucauaa gggcaaccaa aucuuccugu cucagaaguu uguuaaauug     3960

uuauaucacg cgaagaggau aagcaauacu auuaacgaga aucaucguaa auacguggaa     4020

aaccacaaga aagaguucga ggaacuguuu uacuauaucc ucgaguucaa ugaaaacuac     4080

gucggugcca agaagaacgg gaaacugcuu aauuccgcuu uucagucaug gcagaaccac     4140

aguauugacg agcuguguag cucuuucauc ggaccuacag gcuccgaacg gaaggguuug     4200

uuugagcuaa cgucaagagg cagugccgca gauuucgagu uccugggggu gaaaauacca     4260

agguaucgcg acuacacccc cagcucccuc cugaaggaug cuacucuuau ucaucaaucu     4320

guuacaggau uauaugaaac ccgaaucgac cuggccaaac ucggcgaggg gaagagaccu     4380

gcagccacaa agaaagcugg acaggcgaag aaaaagaaau aaggacuagu guccaccugu     4440

cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac     4500

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa gaauu                                                      4575


<210>  3670
<211>  3651
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3670
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaugg uacgccagcc ugauguccgc gcaucaccuc      180

aggguuggaa uugacguggg uacccauucu guaggccuug cuacacugcg gguggaugac      240

cacgggacuc caauagaguu guuaucagcc cugagucaua uccacgauag cggagucggc      300

aaggaaggua aaaaggacca cgauacgcgc aagaaacuau ccgggauugc acgacgugcc      360

agaaggcucc ugcaucaccg gcgcacccag cuucaacagc uggacgaggu guugagagau      420

cucggauucc cgauccccac accuggcgaa uuucuggacc ugaacgagca gacugaucca      480

uauaggguuu ggcgggugcg cgcucgacuc gucgaagaga aguuacccga ggaacugaga      540

ggcccugcaa uuucuauggc cgugaggcau aucgcucggc accgugggug gcgcaaucca      600

uacucgaaag uagagucacu uuugagcccc gccgaagaga guccuuucau gaaggcgcug      660

agagagagga uacuagcaac cacaggagaa guucucgacg augguaucac cccaggccag      720

gccauggcuc aaguggcccu gacucacaac auuuccaugc gggggccgga gggaauccuu      780

ggcaaacugc aucagucuga caaugcaaac gaaauucgaa agaucugcgc ucgccagggu      840

gucagccccg auguguguaa gcaguugcuc agagccgugu uuaaagcgga cuccccuagg      900

gggucagccg ucagucgggu ugcaccagac ccccugccug gacaaggcag cuuccgcaga      960

gcuccaaagu gcgaucccga guuucagagg uuccguauaa uuucuaucgu ggccaauuua     1020

cggauuuccg aaacaaaagg cgagaaccga ccgcugaccg cugacgagag aaggcacgua     1080

gugacguuuc ucacugaaga uucacaggcc gaccuuaccu gggucgaugu ggcagagaag     1140

cuggggguuc aucgccggga cuugagagga acagccgugc acacugauga cggugaaagg     1200

agcgcugcgc gcccucccau cgaugcaacc gaccgaauca ugcggcagac aaaaauuagu     1260

ucucuaaaga ccugguggga ggaagccgau uccgagcaac guggcgcuau gauaagauau     1320

cuguacgagg acccaacgga cagcgaaugu gccgagauca uugcagaacu cccugaggaa     1380

gaucaggcca aacuggacuc gcuucaucug cccgcuggga gggccgcaua uucucgcgag     1440

uccuugacug cgcucucaga ucacaugcug gcuacaaccg acgauuuaca cgaggcccgg     1500

aagagacugu ucggagucga cgauaguugg gcaccacccg ccgaagcuau caaugccccu     1560

gugggcaacc caagcguaga caggacucua aagauaguug gucgauaccu cuccgcagug     1620

gagucuaugu gggggacacc ggaagucauu cauguggagc acguccgcga uggcuuuacc     1680

agcgagcgga uggcugacga aagagauaaa gccaauaggc gucgcuauaa cgacaaucag     1740

gaggcgauga agaaaaucca acgggauuac ggaaaggaag gcuacaucuc aagaggggac     1800

auugugaggc uggacgcccu ugaguugcag ggaugcgcuu gucuguauug cgguacgacc     1860

aucggcuacc auacauguca gcucgaucac auuguucccc aggcagggcc uggaaguaac     1920

aaucgacggg gcaaccuggu ggccguaugc gaacgcugua auagauccaa aagcaacacu     1980

ccauucgcug ucugggccca aaagugcggu auaccccacg uggggguuaa ggaggcaauc     2040

ggcagggugc ggggauggcg aaaacagacc ccuaacacau cuucagagga ccuuacuaga     2100

cugaagaaag aagucauugc gcgcuugagg cguacccagg aggauccaga aaucgacgag     2160

cggaguaugg aaagcguggc cuggauggcu aaugagcucc aucacagaau cgccgcagcu     2220

uaucccgaga caaccguaau gguguacagg ggcuccauua cugccgcagc ccgcaaggcu     2280

gcggggauag auucucgaau caaccugauu ggagaaaaag guagaaagga ccggaucgau     2340

aggcgccauc acgccguuga cgcaucgguc guggcuuuaa uggaggccag cguggccaag     2400

acgcuggcug aacguuccuc acuaagaggc gagcagcggc ucacagggaa agaacaaacc     2460

uggaagcagu auacuggaag uaccgucggc gcaagggagc auuuugagau guggcgcggu     2520

cacaugcugc accuuacaga auuguucaau gagcgacugg ccgaagauaa aguuuacgug     2580

acccagaaca uuagacuccg gcugucugac gggaaugcgc auacugugaa cccgagcaag     2640

uuaguauccc acaggcuggg cgauggacuu acaguccagc aaaucgaccg cgcuuguacg     2700

ccugccuugu ggugcgcacu caccagagag aaagauuuug acgagaagaa uggccugcca     2760

gccagggaag accgggcuau acgugugcau gggcacgaga ucaagucaag cgauuauauu     2820

cagguuuucu cuaaacgcaa gaaaacugac uccgaucgag acgaaacacc cuucggagca     2880

aucgccguga gaggcgguuu ugucgagauu gggccuagua uccaucacgc gaggauauac     2940

cggguggaag gaaagaaacc cguuuacgcu augcuacgcg uguucaccca cgaucugcuc     3000

agccagagac auggcgaccu guuuucugcc guaauuccac cucaguccau cucaaugagg     3060

ugcgcagagc ccaaacuucg gaaagccauu acaacuggua acgcuaccua uuugggcugg     3120

gucguggugg gggaugagcu ggaaaucaau gucgacaguu ucacgaagua cgccauagga     3180

cgauuuuuag aggauuuccc aaacaccaca agauggcgca ucuguggcua ugacacuaau     3240

agcaagcuca cccugaaacc gauuguucuu gcagcugaag ggcuggagaa cccuucgucc     3300

gccgugaaug aaaucguaga guugaaggga uggagggugg cgauuaacgu ccucacaaaa     3360

gugcacccca cuguugugcg gcgugaugcc cugggcagac caagguacuc uagccgcuca     3420

aaucuaccca ccaguuggac gaucgagaag cggccugcag cuacaaagaa ggccggucaa     3480

gcuaagaaaa agaaauaagg acuagugucc accugucccu ccugggcugc uggauugucu     3540

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              3651


<210>  3671
<211>  4512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3671
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaacc aagccauaca gcauuggacu ggacauaggu      180

acaaacuccg uuggcugggc cgugacuacg gauaauuaua aaguaccguc uaagaagaug      240

aaagugcucg ggaacaccuc aaagaaauac aucaagaaaa aucuucuggg agucuuguua      300

uucgacagug gcauuacagc ggaggguagg cggcugaagc gcacugcucg acguagauau      360

accaggcggc gcaacagaau ccuauaccuc caggaaauuu uuagcacaga gauggccacc      420

cuggaugacg cauucuuuca aaggcuugau gacuccuucc uggugcccga ugacaagcgg      480

gauucuaaau auccuaucuu ugggaauuug guugaagaga aggccuacca ugacgaguuc      540

ccaacuauau aucaccuccg caaauaccug gcugauucga caaagaaagc agaccugcga      600

cucguguacu uagcccuggc ucauaugauc aaguauagag gacacuuucu uauugagggc      660

gaguucaacu caaagaauaa cgauauccag aagaauuuuc aggacuucuu ggacaccuac      720

aacgccauuu uugaaagcga ucugagucua gaaaauucca agcagcucga agagaucguc      780

aaagacaaga uaucuaaacu ggagaagaaa gauaggauuc uuaagcuguu ccccggcgaa      840

aagaacagcg ggaucuuuuc cgaguucuug aaacucauug ugggaaauca agcggacuuc      900

cggaagugcu uuaaccugga ugaaaaagca ucauuacauu ucaguaagga gagcuaugac      960

gaagaucugg agacgcuccu ugguuacauc ggcgacgauu auucugacgu auuucugaaa     1020

gccaagaagu uguacgaugc uauccuacug uccggguucc ucacuguuac cgacaaugag     1080

acagaagccc cucugucaag cgcaaugauu aaacguuaua acgagcacaa ggaagaccuu     1140

gcucuguuga aagaguacau acgcaacauc agucucaaga cuuacaauga aguguuuaaa     1200

gaugacacca agaacggaua ugccggcuac auugauggua agacaaauca ggaggacuuc     1260

uaugucuacc ugaaaaaguu acuggcggag uuugaagggg ccgauuauuu ccuagagaaa     1320

aucgacagag aagauuuucu caggaagcag cggaccuucg acaacggauc uauaccauac     1380

cagauucacc uucaagagau gcgagcaauc cuugauaaac aggcuaaguu uuaucccuuc     1440

uuggccaaaa auaaggaacg caucgagaag auucugacgu uuagaauccc uuacuacgug     1500

ggcccacucg cuaggggcaa cuccgacuuc gccuggagca uucggaaacg caaugagaag     1560

auaacuccgu ggaacuucga agaugugauc gacaaagagu cgucugcaga agccuuuauu     1620

aauagaauga cauccuucga ucuguaucuu cccgaggaga agguccugcc uaaacauuca     1680

uugcucuacg aaaccuuuaa cguuuauaau gagcugacua aggugagguu caucgcugaa     1740

aguaugcgug acuaccaguu uuuagacagc aagcagaaaa aggauaucgu acggcuguau     1800

uucaaagaca agcgaaaagu gacagauaag gacauuauag aguaccuaca cgcgaucuau     1860

ggguacgaug gaauugaacu caaggguauc gagaaacaau uuaacuccuc ucugagcacc     1920

uaccaugacc uuuugaauau uaucaacgau aaggaguucc uggacgauuc aaguaaugaa     1980

gcaauaaucg aggaaauuau ccacacgcuc accauuuuug aggacagaga aaugaucaaa     2040

cagaggcugu ccaaguucga gaacauauuu gauaaaagcg ucuuaaagaa gcugucucgc     2100

cggcacuaua caggcugggg gaaacuuuca gccaaguuga uuaacggaau cagagacgag     2160

aaaaguggca auacuauucu cgauuaccug aucgacgacg guauaagcaa caggaauuuc     2220

augcagcuaa uccaugauga cgcucugucc uuuaagaaaa agauucaaaa agcccagauc     2280

auuggggaug aagacaaggg caacaucaag gaggugguua aaucucuccc aggaucgccc     2340

gcaauaaaga aaggcauucu gcagagcauc aagaucgugg augaacuugu caaagugaug     2400

ggagggcgca agccugaguc cauuguaguu gaaauggccc gagagaauca guauaccaac     2460

caagguaagu caaauaguca gcagcgguug aaacgucugg agaagucuuu aaaagaacuc     2520

ggcagcaaga uccugaaaga gaacauucca gcuaagcugu ccaagauaga caauaacgcc     2580

cugcagaaug auagauugua ccucuauuac cugcaaaacg ggaaagacau guauacagga     2640

gaugaccuag auaucgacag gcugucaaau uacgacauug aucacaucau uccccaggca     2700

uuccucaagg acaacagcau cgauaauaaa gugcuggucu cuuccgcgag uaaccgcggc     2760

aagagcgacg augugccguc ucuugaaguc gugaaaaagc ggaagacuuu cugguaccag     2820

uugcugaaau ccaaguuaau aucacagaga aaauuugaca accucaccaa ggcugagagg     2880

ggugggcuga guccugagga uaaagccggc uucauccaac gacagcuugu ugaaacacgc     2940

cagauuacca agcauguggc acggcugcua gacgagaagu uuaauaacaa aaaggaugaa     3000

aauaacagag ccguaaggac ugucaaaauc auuacgcuca agagcacacu ggugucgcag     3060

uuccguaaag acuuugaguu guauaagguu cgcgaaauca augauuucca ccaugcucac     3120

gacgccuacc ugaacgcagu ggucgcuucc gccuuacuca aaaaguaucc caagcuggag     3180

ccagaguuug uguacggaga uuauccuaaa uacaauucuu uccgggaaag aaagagcgcg     3240

accgagaaag uauauuuuua cucaaacaua augaauauuu ucaagaaaag uaucucccuu     3300

gccgacggca gggugaucga acgaccccug auugagguua acgaagagac uggggagucu     3360

gucuggaaua aggaaagcga cuuggcuacc gugcggcgcg ugcucuccua cccacaaguc     3420

aacguuguga agaaagugga ggaacagaau cacggacugg auagagguaa gcccaaaggc     3480

cuauuuaacg caaaucuguc aagcaagccu aaaccaaaca guaacgagaa ucuuguaggg     3540

gccaaggaau aucucgaccc gaagaaauac ggaggcuaug cugguauauc uaacuccuuc     3600

acaguccugg ugaaggggac caucgagaaa ggcgccaaga aaaagauuac uaauguuuug     3660

gaguuccagg gaaucagcau ucuggauagg aucaacuacc ggaaggacaa auuaaauuuu     3720

cuccuggaaa agggcuauaa agauauagag cuuaucauug aacugcccaa guacucguug     3780

uucgagcucu cagacggguc ucgaagaaug cuggcaagua uccuauccac aaacaauaaa     3840

cgcggagaga uucauaaggg caaccaaauc uuucugagcc agaaauucgu gaaacuucuc     3900

uaucacgcga agaggauauc uaauacgauu aacgaaaauc aucguaaaua cgucgagaac     3960

cacaagaaag aauuugagga acuguucuac uauaucuugg aguuuaauga gaacuacgug     4020

ggugccaaga aaaaugggaa guuacugaac agcgcuuucc aguccuggca gaaucacuca     4080

aucgaugaac ucuguaguag cuuuauugga ccuaccggcu cugagcggaa gggucuguuc     4140

gaacuuacuu ccagaggcuc agccgcagac uuugaguucc uggggguuaa aaucccaagg     4200

uaucgcgauu acacacccag uagcuugcua aaggacgcua cccugauaca ucaauccgug     4260

acaggacucu augaaacucg aauugaucug gccaaacuug gcgaggggaa gagaccugca     4320

gccaccaaaa aggcuggaca ggcgaagaaa aagaaauaag gacuaguguc caccuguccc     4380

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     4440

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaagaa uu                                                         4512


<210>  3672
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3672
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcagcc gcguucaagc caaacagcau uaauuacaua      180

cugggacucg acaucgguau ugcuuccguu ggcugggcca ugguggagau cgaugaagag      240

gaaaacccga uuaggcuuau cgaccugggg guacgggugu uugagcgcgc agaagucccc      300

aaaaccggag auucuuuggc cauggcucga cguuuagcaa gaucagugag gcggcugaca      360

cgcagaaggg cccaucggcu acuccgcacu cgaagacugc uuaagaggga gggcguucug      420

caggcugcca auuucgacga gaacgguuug auaaagaguc ucccuaauac gccauggcaa      480

cugcgggcgg cagcccugga ucguaaacuc acccccuuag aauggagcgc ugugcugcuu      540

cacuugauca agcaucgcgg guaucugucc cagaggaaaa acgagggaga aacagccgac      600

aaggagcuag gcgcacuccu gaaaggcguc gcugggaaug cccacgcgcu ucagacugga      660

gauuuuagga ccccugccga gcuggcauug aacaaguucg aaaaggaguc uggucauauu      720

cggaaucagc gaucggacua cucacacaca uuuagccgca aagaucucca agcugaacug      780

aucuuacugu ucgagaagca gaaagaauuu ggcaacccac acgugagugg gggacucaag      840

gagggcauug agacccuucu gaugacucag agacccgccu uguccgguga cgcuguacag      900

aaaaugcuag ggcauugcac auucgaaccu gccgagccaa aggcagccaa aaauaccuau      960

acggcugaaa gguuuaucug gcugacuaag cucaacaauc ugcggauacu ugagcaagga     1020

ucugaacgcc cgcugaccga uacagagaga gcgacuuuga uggacgagcc cuacagaaag     1080

agcaaacuca ccuaugcaca ggcccguaag cuguuaggcc uggaagauac agcuuucuuu     1140

aaaggccuac gguacgggaa ggacaacgcc gaggcaucca cccucaugga aaugaaagcc     1200

uaucacgcua uuucacgagc ccuggagaag gaaggacuua aggacaaaaa gaguccuuug     1260

aaucugagcc cagagcucca ggaugagauc gguacggcau ucucucuguu uaaaacugac     1320

gaagauauua caggcagacu uaaggacagg auccagcccg agauccugga agcguugcuc     1380

aaacauauuu ccuucgauaa guucguucaa auaucacuga aggcuuuacg ccggaucgug     1440

ccucugaugg agcaggggaa aagauacgac gaggccugug cagaaauuua cggagaucac     1500

uauggcaaga aaaacaccga ggaaaagauc uaccuaccac ccauaccggc cgacgagauu     1560

aggaacccug ucgugcuccg cgcucugagc caggcccgaa aagugaucaa uggugucguu     1620

cggcguuaug ggagucccgc aagaauccac auugaaacug cuagggaggu gggcaagucu     1680

uuuaaggauc gcaaagagau cgaaaagcgg caagaggaaa acagaaaaga cagggagaag     1740

gccgcggcca aauuccgaga auacuuucca aauuucguag gagagccuaa guccaaagau     1800

auucuuaagu ugcgccugua ugagcagcag cauggcaagu gccucuacag cgggaaagaa     1860

auaaaccugg gacgguuaaa ugagaagggu uauguggaaa ucgaccacgc ucugcccuuu     1920

ucgagaacau gggacgauuc uuucaacaau aaaguccuug uguugggcuc cgagaaccag     1980

aauaagggga accaaacccc auacgaauac uuuaauggaa aagacaacuc aagggagugg     2040

caggaguuca aggcacgugu ugaaacgagu cgcuuucccc ggagcaagaa acagaggauu     2100

cuccugcaga aguucgauga ggacggcuuu aaagaaagaa aucuaaacga uacccgauau     2160

gugaaucggu uccuguguca auuugucgcc gaccgcauga gacucacagg uaaggggaaa     2220

aagagggugu ucgcuuccaa cggccagauc acuaaccugc uucggggauu cuggggcuug     2280

cgaaagguaa gagccgagaa ugaucgccau cacgcacugg acgcgguugu ggucgccugc     2340

ucuaccgugg cuaugcagca gaaaaucaca agguuugucc guuacaagga gaugaacgcc     2400

uucgauggga aaacuauuga caaggaaacc ggagaggugu uacaucagaa aacacacuuu     2460

ccucagccau gggaguucuu ugcacaggaa guuaugauac ggguguucgg uaagccggau     2520

ggcaagcccg aauuugagga ggcugacacc cucgaaaaac ugagaacucu ucuggccgag     2580

aaguugagcu caaggccuga agcaguacac gaguauguca cgccacucuu cgugagucgc     2640

gcccccaauc gaaaaauguc cgggcaggga cauauggaaa caguuaagag cgcuaaaaga     2700

cuggaugagg gcgugucugu ccuacgggug ccucugaccc aacucaagcu gaaagaccuu     2760

gagaagaugg uaaacaggga acgcgagcca aaguuguacg aagcgcugaa agcccguuua     2820

gaggcacaca aggacgaucc cgcuaaagcc uuugccgaac cguucuauaa guacgacaaa     2880

gcugguaaua gaacucaaca ggugaaggca guucgggucg agcaagugca gaagaccggg     2940

guguggguca ggaaccauaa uggcaucgcc gauaacgcga caaugguucg cguggacgug     3000

uuugagaaag gagauaagua uuaccucgua ccuauuuacu cauggcaggu cgcuaaaggc     3060

auccugccag accgagccgu gguucagggg aaggaugaag aggacuggca acuuauugau     3120

gacaguuuca auuuuaaauu cagccugcac cccaacgauc uaguggaagu caucaccaag     3180

aaggcaagaa uguucggaua uuuugccucc ugucaccggg gcacugguaa uauaaacauc     3240

aggauucaug accucgauca caaaaucggg aagaauggca uucuggaggg aaucggugug     3300

aaaacagcuu ugucuuucca gaaguaccag auagacgagc ugggcaaaga aauucgcccu     3360

ugcagauuaa agaagaggcc cccaguucgg aaacguccug cagccacgaa gaaagcgggg     3420

caggcuaaga aaaagaagua aggacuagug uccaccuguc ccuccugggc ugcuggauug     3480

ucucguuuuc cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           3594


<210>  3673
<211>  4350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3673
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaag aaaccauaca ccauuggacu ggacauaggu      180

acaaacagcg uuggcugggc cgugcucacu gaucaguaug accuuguaaa gaggaagaug      240

aaaaucgcgg gggauuccga gaagaaacaa auuaagaaaa auuucugggg agugcggcug      300

uuugacgaag gccagacggc ugccgaucgc cgaauggcac guaccgccag aaggcggauc      360

gagcgcagaa ggaaccggau uucuuacuug caggguaucu ucgcugaaga gaugucaaag      420

acagacgcaa acuuuuucug ccgcuuaagu gauagcuuuu augucgacaa ugaaaagcga      480

aauuccagac auccguucuu ugccacuaua gaggaggaag uggaguacca caaaaacuau      540

cccaccaucu accaucugag ggaggagcua guuaauucuu cggaaaaggc ugaucuccgg      600

cugguguauc uugcccuggc gcacauuauc aaauaccgug ggaacuucuu gauugaagga      660

gcacucgaca cacagaauac cucagucgau ggcaucuaca agcaauuuau acagacuuau      720

aaccaggugu ucgccagcgg cauugaggac gggagucuga aaaagcugga agauaauaaa      780

gacguagcua agauccucgu ugagaaggug acacgcaaag aaaaguuaga gaggauucug      840

aaacuuuacc cuggagagaa guccgccggu auguuugcac aguucaucuc uuugaucguc      900

ggcagcaaag ggaacuuuca aaagccauuc gaccugauug aaaaguccga uauagagugu      960

gcuaaagacu cauaugaaga ggaucuagaa agucuccugg cccuuaucgg agacgaguac     1020

gcggagcugu ucguggccgc aaagaaugcu uauagcgccg uggucuuguc uuccauuauc     1080

accguugcug aaacggagac uaacgccaaa cucucagcaa gcaugauaga aagguuugau     1140

acccaugagg aagaccuggg cgaguuaaag gccuucauua aacugcaccu ccccaagcac     1200

uacgaggaaa ucuuuaguaa uacagagaag caugguuaug cuggguacau cgauggaaaa     1260

acuaagcagg cggacuucua caaauauaug aagaugaccc uugaaaacau ugagggcgca     1320

gauuacuuua ucgccaaaau ugagaaggaa aacuuccugc ggaagcagcg aacauuugac     1380

aauggcgcua uaccucacca guugcaucua gaggaacugg aggccauccu ccaccaacag     1440

gcaaaauauu acccauuccu gaaggaaaac uaugauaaaa uuaagucucu ugugaccuuu     1500

cgcauccccu acuucguagg gccucuggcc aauggacagu ccgaguuugc uugguugacg     1560

agaaaagccg acggugagau caggccaugg aacauugaag agaaggugga cuucggcaaa     1620

agcgcagucg auuuuauaga aaagaugacu aauaaggaca cauaucuccc gaaagagaac     1680

gugcugccca agcacucguu augcuaccaa aaauaccugg uuuauaauga acuaaccaag     1740

gugcgguaca ucaacgauca ggggaaaacu ucuuauuucu ccggacagga gaaggagcag     1800

auuuucaaug accucuuuaa gcaaaaacgc aaggucaaaa agaaagaucu ggaacuuuuc     1860

uugagaaaca ugucacaugu ggagaguccu acaaucgaag gccuggagga cagcuuuaau     1920

uccucuuaca gcaccuauca cgaucuccug aagguaggua uuaagcagga aauccuugac     1980

aacccaguua auacggagau gcuggagaac auagugaaaa ucuugaccgu cuucgaagau     2040

aagaggauga uuaaagagca gcuccagcaa uuuucagacg ugcuggaugg ggucguguua     2100

aagaaacugg aacgucggca uuacacaggc uggggacgac uaagugcgaa gcuccugaug     2160

ggcaucagag acaagcaguc ccaccuuacu auuuuggauu aucugaugaa ugacgacggg     2220

cucaacagga accugaugca guuaaucaau gauagcaacc ugucuuucaa aucaauaauu     2280

gagaaggagc agguuaccac agcugacaaa gauauccaaa guauuguggc cgaccuugca     2340

ggaagccccg ccaucaagaa agguauauug cagucccuca agaucguaga ugaacugguc     2400

ucugugaugg gcuacccucc acagacuauu guuguggaga uggcucgcga aaaucagacc     2460

acagggaaag gaaagaacaa uucgcggccc agauacaaga gccuagagaa agccaucaag     2520

gaauuuggcu cccaaauucu gaaagagcau ccgaccgaca accaggagcu caggaauaac     2580

cgccuguauc uuuacuauuu gcagaauggu aaggauaugu acacugggca agaccuggau     2640

auccacaacu uaucaaauua ugacauagau cacauugucc cucagaguuu caucacggac     2700

aacucuaucg auaaucucgu gcugacaagc uccgcaggca accgagaaaa aggagacgac     2760

guacccccac uugagauugu gcggaagcgu aaggucuuuu gggaaaaacu guaccagggc     2820

aauuugaugu caaagagaaa auucgauuau cucaccaagg cugagagagg gggacugacu     2880

gaagccgaca aagcgcgcuu uauccaucgg cagcuaguug agaccagaca aauuacaaag     2940

aacguggcca acauacugca ccagagguuc aauuacgaga aggaugacca ugguaacacc     3000

augaaacagg ugcgaaucgu cacucucaag agcgcucugg uuucucaguu ucgcaaacaa     3060

uuccagcuuu acaaggugcg ggaugugaau gacuaucacc acgcacauga ugccuacuug     3120

aacggcguag ucgcuaauac acuguuaaaa guguauccuc agcucgaacc cgaguucguu     3180

uacggggacu aucaccaguu ugauugguuc aaggccaaca aggcaacggc gaaaaagcaa     3240

uuuuacacca auauuaugcu guucuuugcc cagaaagaca gaaucauuga ugaaaacgga     3300

gagauccuuu gggacaagaa auaucuggac acugugaaga aggucauguc cuacaggcag     3360

augaauauag ugaaaaagac agaaauccaa aaaggcgagu ucaguaaggc uaccauuaaa     3420

ccaaagggua acagcucuaa acuaaucccc cguaagacaa auugggaucc uaugaaguac     3480

gggggccucg acuccccaaa cauggccuau gcaguuguga uugaguacgc uaaaggaaag     3540

aauaaacugg uauuugaaaa gaaaaucaua cgcgucacua uuauggagcg gaaggccuuc     3600

gaaaaggaug agaaagcauu uuuggaggaa cagggcuaua gacagccgaa ggugcuggcc     3660

aaauuaccca aguacacccu cuaugagugu gaagagggga ggcgacggau gcuggcuuca     3720

gcgaacgaag cccagaaagg aaaucaacag gugcuuccua accaucuggu cacguugcuc     3780

caccaugcag cuaacugcga gguuagugac ggcaagagcc uggauuacau cgagucgaau     3840

cgcgaaaugu ucgccgagcu acuggcccac guguccgaau uugcuaagag auauacccuu     3900

gcagaggcca accucaauaa aaucaaccag cuguucgaac agaauaagga gggugacauu     3960

aaagcgauag cucaaucuuu cguagauuug auggccuuua acgcaauggg ggccccagcu     4020

agcuucaagu uuuucgagac aacuaucgaa aggaaacggu acaauaaccu gaaggaguua     4080

cucaauucaa ccauuaucua ccagaguauu acaggacugu augaaucccg aaagagacuu     4140

gacgauaaac gccccgcagc cacuaagaaa gcgggccagg cuaagaaaaa gaaauaagga     4200

cuagugucca ccugucccuc cugggcugcu ggauugucuc guuuuccugc caaauaaaca     4260

ggaucagcgc uuuacagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4350


<210>  3674
<211>  4383
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3674
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaag aaaccauaca gcauuggacu ggacauaggu      180

accaacuccg uuggcugggc cgugguaaca gaugacuaua aggugccggc gaagaaaaug      240

aagguccucg ggaauacuga uaaaucucau aucgagaaga accuucuggg agcuuuguua      300

uucgacucag gcaauacggc cgaagauagg cggcugaaac gcaccgcacg acguagauac      360

acaaggcggc gcaaccggau ucuauaucuc caggagaucu uuagugaaga gauggguaag      420

guggacgaua gcuucuuuca caggcuggaa gacuccuucc uuguuacuga ggauaagaga      480

ggggagcgcc aucccauuuu uggaaaucug gaagaggaag ugaaauacca cgagaacuuc      540

ccuaccaucu aucauuugcg acaauaccuc gccgacaauc cagagaaggu cgaucugaga      600

cugguguauc ucgcuuuagc acacauaauc aaauuuaggg gccacuuccu gauugaaggc      660

aaguuugaca cacggaacaa ugauguacag cgucuuuucc aggaguuuuu ggccguuuac      720

gacaacaccu ucgaaaauuc uucgcugcag gagcaaaacg ugcaggucga agagauccua      780

acugacaaaa uuucaaagag cgcuaaaaag gaucgcgugc ucaagcuguu ucccaaugag      840

aaaaguaacg ggagauucgc cgaguuccuu aagcugaucg ugggaaauca ggcggacuuu      900

aaaaagcauu ucgaguugga agagaaagca ccucuccagu uuuccaagga uacauacgaa      960

gaggagcugg aagucuuacu ggcccaaaua ggugacaacu augcugaacu cuuccuuucu     1020

gccaagaaac uguacgauag cauuuugcua uccggcaucc ugaccguuac ggacgugggg     1080

acuaaggcac cacucucagc uaguaugauu cagagguaua acgaacacca gauggaucug     1140

gcccagcuua aacaauuuau ccggcagaag cugagcgaca aauacaauga gguauucucu     1200

gaugugucca aggacggaua ugcgggcuac aucgauggua agaccaacca ggaagccuuu     1260

uauaaauacu ugaaggggcu ccugaauaaa auugagggau caggcuacuu cuuagacaag     1320

auagagcgag aagacuuucu gcgcaaacaa agaacauucg auaacggcag caucccccau     1380

cagauucacc uacaggagau gagggcaauc auacggcgcc aggcugaauu uuauccuuuc     1440

cucgccgaca aucaagauag aauugagaag cugcuuacuu uuaggauccc auacuauguc     1500

gggccguugg cucguggaaa gagugacuuc gccuggcugu cucggaaauc cgcagauaag     1560

aucacccccu ggaacuucga cgagauugug gauaaagaaa gcucggccga ggcuuuuauc     1620

aaucgaauga caaacuacga ccucuaucug ccuaaucaga agguucuucc aaaacacucu     1680

cuguuguacg aaaaguucac cguguauaac gagcucacga aagucaagua caagacugaa     1740

caggguaaaa cagcguuuuu cgaugcaaau augaagcagg agauuuuuga cggcguguuc     1800

aaaguauaca gaaagguuac caaagauaag cugauggacu uuuuagagaa ggaguucgau     1860

gaauuuagga uaguggaccu gacugggcua gacaaagaaa acaaggucuu caaugccucc     1920

uauggaacau accaugaucu cugcaaaauc cuggacaagg auuuucuuga caacaguaaa     1980

aaugagaaga uuuuggaaga uaucgugcug acccucacgc uguucgagga ccgcgagaug     2040

auccggaaga gauuagaaaa cuauucagau cugcuuacca aagagcaagu caagaaauug     2100

gaaaggcgcc acuacacagg cuggggucga cucagcgcug agcugauuca ugggauacgg     2160

aacaaggaau cccguaaaac uauccuagac uaucugauug augacggcaa uucuaacaga     2220

aauuuuaugc agcucaucaa cgaugacgcc cugagcuuca aggaggagau ugcaaaggcc     2280

caggugaucg gagaaaccga uaaucuuaac cagguugugu cagacauagc uggcaguccc     2340

gccaucaaaa aggggauuuu gcaaucccug aaaaucguag acgaguuagu caagauuaug     2400

ggacaccagc cugaaaauau cgugguugag auggcaaggg agaaccaguu cacaaaucag     2460

ggucgccgga acagccaaca gagacucaaa ggccugacug auucuauaaa ggaauuuggg     2520

ucacagauuc uuaaagagca uccaguggaa aauagucaac ugcagaacga cagguuguuc     2580

cucuacuauc ugcagaaugg acgagauaug uacaccggcg aggaacuaga caucgauuac     2640

cugagccagu augacauuga ucacaucaua ccccaagcgu uuaucaagga caacuccauu     2700

gauaaucgcg uccucacauc uucgaaggag aaccggggua aaagcgacga ugugccgucc     2760

aaggacguag ugagaaaaau gaagucauac uggaguaaac ugcuuucugc uaaguugauc     2820

acccagagga aguucgacaa ccugacuaaa gccgagcgug ggggcuuaac ggaugacgau     2880

aaggcaggau uuauuaaacg ccagcucguu gaaacacggc agaucaccaa gcacgucgcc     2940

agaauacugg acgagagguu caauacugaa accgaugaga acaauaaaaa gauucgacaa     3000

gugaagaucg ugacacuuaa aagcaaccug gucuccaauu uucggaagga guucgaacua     3060

uauaaaguuc gcgagaucaa cgacuaccau cacgcucaug augccuaucu caaugcagug     3120

auuggcaagg cucuguuggg gguguacccu cagcuggaac ccgaguuugu auauggagac     3180

uacccacacu uccacgguca uaaagaaaac aaggccaccg cgaagaaauu uuucuacuca     3240

aauaucauga acuucuuuaa gaaagaugac gucagaacug auaagaaugg cgagauuaua     3300

uggaaaaagg acgaacacau cagcaacauu aagaaagugu uaucuuaucc ucagguuaau     3360

aucgugaaga aagucgagga gcagacaggg ggauucucca aggaaaguau ucuccccaaa     3420

ggcaacagcg auaagcugau cccaaggaaa acgaagaagu uuuacuggga caccaaaaag     3480

uauggugggu ucgacucucc cauaguggcc uacuccaucc uuguuauugc ugauaucgag     3540

aaaggcaaga guaaaaagcu gaagacugug aaagcauugg uaggagucac aauuauggaa     3600

aagaugaccu uugagcggga cccuguggcc uuccucgagc gaaaaggcua uagaaaugug     3660

caagaagaga acaucauaaa gcugccaaaa uacucacuau uuaagcugga aaacgggcgc     3720

aagaggcuuc ucgcuagcgc ccgugagcug cagaaaggaa augaaauugu cuugccgaac     3780

caucugggca cauuacucua ucacgcaaag aauauccaua aaguugauga gcccaagcac     3840

cuggacuacg uggauaaaca caaggacgag uucaaggaac uucuggaugu agugucgaac     3900

uuuuccaaaa aguacacuuu ggcggagggu aaucucgaga aaaucaagga acuguaugcc     3960

cagaacaaug gggaagaccu aaaagagcug gcuucuagcu ucauuaaccu ucucaccuuu     4020

acggccauag gagcuccugc aaccuucaag uuuuucgaua aaaauaucga ccggaagaga     4080

uacacaucaa cuaccgagau ucugaacgcc acauugaucc aucaaaguau uacuggcuua     4140

uaugaaacca ggaucgaucu gaauaagcuc gguggcgaca aacgcccagc agccacgaag     4200

aaagcugggc aggcgaagaa aaagaaguaa ggacuagugu ccaccugucc cuccugggcu     4260

gcuggauugu cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     4380

auu                                                                   4383


<210>  3675
<211>  3516
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3675
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcacag acaacaaacc ugagcuacau ucucggacuu      180

gaccugggua uagccuccgu uggcugggcg gugguagaga ucaaugaaaa cgaggaucca      240

auuggguuga ucgacguggg agucaggauu uucgaacggg cugaggugcc gaagacuggc      300

gaaucuuuag cccugucacg ccgacuagca cguaguacga gaaggcucau ccggcgcaga      360

gcccauaggc ugcuucuggc uaaacgguuu uugaagcgcg aggguauacu cagcaccauc      420

gaucuggaga aggggcugcc caaucaagca ugggaacucc gaguugccgg auuagagaga      480

aggcuguccg cuauugaaug gggcgccgug cuuuugcacc ugaucaaaca ucggggcuau      540

cuaucuaagc guaaaaacga gucgcagaca aauaacaagg agcucggggc gcugcuuuca      600

ggagucgcac agaaucacca gcuguugcaa agcgacgauu accgcacucc ugccgaacuc      660

gcucugaaaa aguucgccaa ggaggaaggu cauauuagaa accagagggg cgcauauacc      720

cacacauuua aucgguuaga ccugcucgcu gagcuuaacc uguuguucgc ccagcagcac      780

caauuuggga auccacauug caaagaacac auccagcagu acaugaccga gcuacugaug      840

uggcagaagc ccgcgcucag uggagaggcc auacugaaaa ugcuuggcaa guguacucau      900

gagaaaaacg aguucaaggc agcuaaacac acauauuccg ccgaacgauu uguguggcug      960

accaaguuga auaaccuccg cauucuggag gauggugcug aaagagccuu aaaugaggag     1020

gaaaggcaac ugcuaaucaa ccacccuuac gagaagucua aacucacgua ugcacaggua     1080

cggaagcugc uuggguugag cgaacaggcc auuuucaaac aucugcgcua cuccaaggag     1140

aacgcugaau cagcgacuuu uauggagcuc aaagcauggc acgccaucag aaaggcucug     1200

gagaaucaag gacuuaagga caccuggcag gaucuggcca aaaagccaga cuugcucgau     1260

gaaaucggca cagcauucag ucuguacaaa acugacgagg auauucagca guauuuaacc     1320

aacaagguuc ccaauagcgu gauaaacgcc cugcuagucu cucucaauuu ugacaaauuc     1380

aucgaacugu cccuuaaguc auugaggaag auucugccuc ucauggagca aggcaaacgu     1440

uacgaccagg cuugccggga gaucuauggg caucacuacg gagaagccaa ccagaagaca     1500

agccagcugu uaccagcaau accggcgcaa gagauucgaa aucccguggu gcugagaacc     1560

cuuagucagg cuaggaaagu caucaacgcc aucauucgcc aguaugguuc uccugcacgg     1620

guucauaucg aaacgggcag agaguugggg aaguccuuua aagaaaggcg cgagauucag     1680

aagcaacagg aggauaaucg aacuaagcgg gaaagcgccg ugcagaaauu caaggaacuc     1740

uucucggacu uuucuuccga accaaaauca aaggauauac ugaaauuccg ucuauacgag     1800

cagcaacacg gaaagugucu guauaguggc aaagaaauca acauucacag acucaaugag     1860

aaggguuacg uagagaucga ccaugcucug cccuuuagca ggacauggga ugacuccuuc     1920

aacaauaagg ugcuugucuu ggccucugaa aaccagaaua aagggaacca gaccccuuac     1980

gaguggcugc aaggcaagau caacagcgaa cgcuggaaaa auuuuguggc auuaguucuc     2040

ggaucacagu gcagugcugc caagaaacag cggcugcuua cucaggugau ugaugacaac     2100

aaguucauag auagaaaucu gaacgacaca agguauaucg cgcgauuuuu guccaauuac     2160

auucaagaga accuccugcu agucggcaag aauaaaaaga acguguucac cccaaauggg     2220

cagaucacgg cccugcuccg cagccggugg ggacugauua aagcuagaga aaacaauaac     2280

aggcaccaug cacuugaugc caucguaguu gcuugugcca cccccucuau gcagcagaag     2340

auaacacguu uuauccgcuu caaagaggug cacccguaua agauugagaa ucgguacgaa     2400

auggucgacc aagagucagg ugaaaucauu aguccucacu uucccgagcc augggcauau     2460

uucagacagg aggugaacau cagggucuuu gacaaucauc cugauacugu guugaaggaa     2520

augcugcccg accgaccaca ggcgaaccac caguucguuc aacccuuauu cgugagccgg     2580

gccccuaccc gcaaaauguc cggccagggg cauauggaga caauaaaguc ugcuaaaaga     2640

cucgccgaag gaauuucggu acugaggauc ccacuuacuc agcugaagcc gaaccuacuc     2700

gagaauaugg ucaacaaaga acgggagccc gcacuguacg cuggcuugaa ggcccgacug     2760

gcagaguuua aucaagaucc ugccaaggcu uucgcgaccc cauuuuauaa acaggguggg     2820

caacagguga aggccauuag aguugaacag gugcagaaaa gcggcgucuu agugcgcgag     2880

aacaauggag uagcagacaa cgcuuccauc gugaggacag auguuuucau aaagaauaac     2940

aaauuuuucc ucguccccau cuacaccugg caaguggcca agggcauucu gccuaauaaa     3000

gccaucgugg cucacaagaa cgaagacgag ugggaagaga uggaugaggg ggcaaaguuu     3060

aaauucucac uuuuuccaaa ugaccugguc gaauugaaga cuaaaaagga guacuucuuu     3120

ggauauuaca uuggucucga ucgugccacg ggcaacauca gucugaaaga acaugacggg     3180

gagauaucua agggaaagga uggcguuuau cgggugggug ugaaacuagc gcugagcuuc     3240

gaaaaguacc agguagacga gcuugggaaa aauagacaga uuugcaggcc ccagcagcgc     3300

cagccggucc gaaagagacc ugcugccacc aaaaaggcag gccaagccaa gaaaaagaaa     3360

uaaggacuag uguccaccug ucccuccugg gcugcuggau ugucucguuu uccugccaaa     3420

uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3516


<210>  3676
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3676
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcagcc gcguucaagc caaacccgau uaauuacaua      180

cugggacucg acaucgguau ugcuagcguu ggcugggcca ugguggagau cgaugaagac      240

gagaacccca uuugccuuau cgaucugggg guaagggugu uugaacgggc agaggucccu      300

aaaaccggag acuccuuggc cauggcucgc cgauuagcac guucugugag aaggcugaca      360

cggcgcagag cccauaggcu acuccgggcu cgccgacugc uuaagagaga aggcguucug      420

caggccgcgg auuucgacga gaaugguuug auaaagucac ucccaaacac ucccuggcaa      480

cugagggcag ccgcucugga ucggaaacuc acgccuuuag aguggagugc cgugcugcuu      540

cacuugauca agcaucgugg guaucugagc cagcgcaaaa augaaggaga gaccgcagac      600

aaggaacuag gcgcucuccu gaaaggcguc gccgauaacg cgcacgcccu ucagacaggg      660

gacuuuagaa cuccagcaga gcuggcuuug aauaaguucg agaaggaauc cggacauauu      720

aggaaccagc ggggugauua cucucacacc uuuucgcgaa aagaccucca agccgagcug      780

aucuuacugu ucgaaaagca gaaagaguuu ggcaaucccc acgugucagg gggacucaag      840

gaaggcauug agacacuucu gaugacccag cgcccugcuu ugagcgguga ugccguacag      900

aaaaugcuag ggcauuguac uuucgagcca gcagaaccga aggccgcuaa aaacacauau      960

accgcggaga gauuuaucug gcugacgaag cucaauaacc ugaggauacu ugaacaagga     1020

agugagcggc cccugacuga caccgaacgc gcaacauuga uggacgagcc uuacagaaag     1080

uccaaacuca cuuaugccca ggcuaggaag cuguuaggcc uggaggauac cgccuucuuu     1140

aaaggccuac guuacgggaa ggacaaugca gaagccucua cacucaugga gaugaaggcu     1200

uaucacgcca uuagccgggc acuggaaaag gagggacuua aggauaaaaa guccccauug     1260

aaccugucac ccgaacucca ggacgagauc gguaccgcgu ucagucuguu uaaaacggau     1320

gaggacauua cuggccgacu uaaggauaga auccagccug aaauccugga ggcuuugcuc     1380

aaacauauua gcuucgacaa guucguucaa auaucucuga aggccuuaag gcgcaucgug     1440

ccacugaugg aacaggggaa acgguacgau gaggcaugcg ccgagauuua cggagaccac     1500

uauggcaaga aaaauacaga agagaagauc uaccuacccc cgauaccugc ugaugaaauu     1560

agaaaccccg ucgugcucag ggcccugucc caggcacgca aagugaucaa cggugucguu     1620

cgacgguaug ggucaccagc ucguauccac auugagaccg ccagagaagu gggcaagagc     1680

uuuaaggaca ggaaagagau cgagaagcgc caagaagaga aucggaaaga cagagaaaag     1740

gcggccgcua aauucaggga guacuuuccu aacuucguag gagaacccaa gaguaaagau     1800

auucuuaagu ugcgacugua ugagcagcag cauggcaagu gucucuacuc ugggaaagag     1860

auaaaucugg gacgcuuaaa cgaaaagggu uauguggaga ucgaccacgc acugccauuu     1920

ucccggacuu gggaugacag cuucaauaac aaaguccuug uguugggcuc ggaaaaucag     1980

aacaagggga aucaaacacc cuacgaguac uuuaacggaa aagauaauuc uagagaaugg     2040

caggaguuca aggccagggu ugagaccucc cguuuuccuc gcucaaagaa acagcggauu     2100

cuccugcaga aguucgacga agauggcuuu aaagagagaa accuaaauga cacgagguau     2160

gugaaccgau uccugugcca auuugucgcu gaucggaugc gccucaccgg uaaggggaaa     2220

aagagagugu ucgccaguaa uggccagauc acaaaccugc uuaggggauu cuggggcuug     2280

cggaagguac gagcagaaaa cgacagacau cacgcgcugg augccguugu ggucgcuugu     2340

agcacugugg ccaugcagca gaaaaucacc cgcuuuguca gguacaagga gaugaaugca     2400

uucgacggga aaacaauuga uaaggagacu ggagaagugu uacaucagaa aacccacuuu     2460

ccacagccgu gggaguucuu ugcucaggaa guuaugauac guguguucgg uaagcccgac     2520

ggcaagccug aguuugaaga ggccgacaca ccagagaaac uccggacccu gcuugcagaa     2580

aagcuguccu cuagacccga ggccguacac gaauauguca cuccuuuguu cgugagcagg     2640

gcuccaaacc gcaaaauguc agggcaggga cauauggaga cgguuaagag ugcgaaacga     2700

cucgaugaag gcguguccgu ccugagagug ccccuaacac aacugaagcu caaagaccug     2760

gagaagaugg uaaaucggga gagggaaccg aaacuuuacg aggccuugaa agcacgccug     2820

gaagcucaca aggaugaccc ugccaaagcc uuugcugagc cauucuauaa guacgauaaa     2880

gcagguaacc guacccaaca ggugaaggcc guuagagucg aacaagugca gaagacuggg     2940

gugugggucc ggaaucauaa cggcaucgcg gacaaugcua ccaugguuag gguggaugug     3000

uuugagaaag gagacaagua uuacuuagua cccauuuaca gcuggcaggu cgccaaaggc     3060

auccucccug aucgcgcagu gguucagggg aaggacgagg aagauuggca acugauugac     3120

gauucuuuca acuuuaaauu cucacuucac cccaaugacc ugguggaggu caucacaaag     3180

aaggcccgaa uguucggaua uuuugcuagu ugccacagag gcaccgguaa cauaaauauc     3240

cggauucaug accuagauca caaaaucggg aagaacggca uucucgaagg aaucggugug     3300

aaaacugcac ugagcuucca gaaguaccag auagacgagu ugggcaaaga gauuaggcca     3360

ugucgccuga agaagagacc ucccguuagg aaacggccag ccgcgacaaa gaaagcuggg     3420

caggccaaga aaaagaagua aggacuagug uccaccuguc ccuccugggc ugcuggauug     3480

ucucguuuuc cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           3594


<210>  3677
<211>  3600
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3677
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cacccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaaag uaccacguug gaauugacgu ggguaccuuc      180

agcguaggcc uggccgcgau agagguggau gacgcuggga ugccaaucaa aacacucucc      240

cuugucucuc acauucauga uucaggacug gacccggaug aaaucaagag ugccgugacu      300

agguuggcaa gcuccggcau ugcccggcgc acgcgacguu uauauagaag gaagcggcgc      360

agacugcagc aacuagacaa auuuauccag aggcaggguu ggcccguuau agagcucgaa      420

gauuacucug acccucugua uccauggaag gugcgggcug agcuugcagc cucguacauc      480

gcugaugaaa aagagcgcgg ggagaagcug ucagucgccu ugcgacacau ugcgagacau      540

aggggauggc ggaaccccua ugcaaaagug agcagucucu accugccuga cggcccaucc      600

gaugccuuca aggcuauccg ugaagagauu aagcgcgccu cuggccagcc cguaccugaa      660

accgcaacag uugggcaaau ggugacucug ugcgagcucg gaaccuuaaa acugagaggu      720

gagggcgggg uccuuagcgc uagguugcag caguccgacu augcccggga aauccaggag      780

auaugucgaa ugcaagaaau uggacaggag cuguaccgca agaucauuga ugugguguuu      840

gcggccgaau caccaaaagg cagugcaagc ucuagagucg guaaggaccc gcuacagccc      900

gggaaaaaua gggcucucaa ggccuccgac gcuuuccagc gguaccgcau cgccgcacug      960

aucggaaacc uuagaguuag gguggauggc gagaaacgua uucugucagu agaggaaaag     1020

aauuuggugu uugaccaccu cgucaaccug acaccuaaga aagagccaga augggugacc     1080

auagccgaga ucuuaggcau ugaucggggg caacugaucg gaacugcuac aaugaccgac     1140

gauggugaac gagcgggcgc aagacccccu acgcacgaca cuaauaggag cauaguuaac     1200

agucgcauug ccccacucgu ggauuggugg aagaccgcuu cugcccuuga gcagcaugca     1260

auggucaaag cccuguccaa ugcugaggug gacgauuucg acagccccga aggggccaag     1320

guacaggcau uuuucgcgga uuuggacgac gauguucacg cuaaacuaga cucgcugcau     1380

cucccggugg gacgggccgc auauucugag gauacacugg ucagacuuac uaggcgcaug     1440

cuguccgacg gcguggauuu guacaccgcc cgacuccaag aauuugguau cgagccuuca     1500

uggacacccc caaccccucg gaucggggaa cccgucggca acccagcugu ggaccguguu     1560

cugaagacgg ugaguagaug guuagagagc gccacuaaga cauggggagc acccgagagg     1620

guaauuaucg aacacguccg cgagggcuuc gugaccgaaa aacgggcuag agagauggau     1680

ggggacauga ggcgacgcgc cgcgcggaau gccaagcugu uucaggagau gcaggaaaaa     1740

cuaaacguuc agggaaagcc uuccagagcu gaucucugga gguaucaauc ugugcagcgu     1800

cagaauugcc agugugcaua cugcgguagc ccaauuacuu ucucaaacag ugagauggac     1860

cacauagucc cgcgcgccgg ccaagggucc acaaauaccc gggaaaaccu gguggcugua     1920

ugucauagau gcaaucagag caaaggaaac acgcccuuug ccaucugggc aaagaacacc     1980

ucuauugagg gcgugucagu uaaggaagcg gucgagagga cacgacacug ggugacugau     2040

accgguaugc ggaguacaga cuucaaaaag uucacuaaag ccguggucga gcgcuuucag     2100

agagcuacca uggaugaaga gaucgacgcc aggagcaugg aauccguugc auggauggcu     2160

aaugagcuuc ggucucgagu ggcccagcau uucgcaucgc acgggacaac cgugagagua     2220

uaucgcggca gcuugacugc cgaagcuagg cgugcguccg gaaucucagg caagcugaaa     2280

uuuuucgacg gggucggaaa gagucggcuc gauagaaggc aucacgccau ugacgcagcu     2340

gugauagccu uuacgucuga uuacguugcc gagacacugg cugugcgcag caaccuuaag     2400

caaucccagg cacaccgaca ggaggccccu caguggagag aguucaccgg uaaagacgcg     2460

gaacaucggg cugccuggag ggucuggugu caaaagaugg aaaaacuguc agcauugcuc     2520

acugaggauc ugcgcgacga ucgugugguu gugaugagca auguaagauu acggcugggc     2580

aacgggucug cccacaagga aaccaucgga aaacuaucca aggucaaacu caguagccag     2640

cugucugugu ccgacauuga uaaggcuuca agugaggcac uuuggugcgc cuugacaagg     2700

gagccaggcu uugaccccaa ggaaggucug ccugcgaauc cagagcgcca uauccgagug     2760

aacgggaccc acgucuaugc uggcgauaau auuggacucu uccccguuag cgccggcucg     2820

aucgcacuga gagggggaua cgccgaauua ggcuccucuu uucaccaugc ucggguguac     2880

aaaauaacua gcgguaagaa accggccuuc gcaaugcuga ggguauauac aaucgaccuu     2940

uugccuuacc gcaaccagga ucucuuuuca guggagcuga agccacaaac gaugaguaug     3000

agacaggcug agaaaaagcu aagggacgcc cuggcgaccg ggaaugccga auaucucgga     3060

uggcuggucg uggacgauga gcuuguugug gacacuucca agauugcaac agaucagguc     3120

aaagcugugg aagccgaguu gggcaccauc cggcguuggc gcguugacgg uuucuuuucu     3180

cccagcaagc ugcgauuaag accucuccag auguccaaag aaggcauuaa gaaagaguca     3240

gcucccgagc ugagcaagau cauagauagg ccaggguggc uuccugcagu gaacaagcug     3300

uucagugacg gaaaugucac aguagugcgg cgcgauucuu ugggcagagu uaggcucgaa     3360

uccacugccc aucugcccgu gaccuggaaa guccaaaagc ggccagccgc uacgaaaaag     3420

gcggggcagg ccaaaaagaa gaaauaagga cuagugucca ccugucccuc cugggcugcu     3480

ggauugucuc guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3600


<210>  3678
<211>  3300
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3678
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcagcc aggauucugg cguucgacau aggaaucagc      180

uccauugguu gggcuuuuuc ugagaacgau gaacucaagg acugcggcgu ucggaucuuc      240

accaaagugg agaauccaaa gacaggggaa ucacuugccc ugccgcgccg auuggcacgu      300

agugccagaa agagguuagc ucggcgcaaa gcaagacuga accaucuaaa gcaccucauu      360

gccaaugagu uuaaacugaa cuacgaagau uaucagagcu ucgacgaguc ccuugcuaag      420

gccuacaaag gaucucugau cucgcccuau gaguugaggu uucgggcgcu caaugaacug      480

cugucaaagc aagauuucgc acgcguaaua cuccauaucg ccaagcgaag aggcuacgac      540

gauauuaaaa acagcgacga uaaggagaaa ggugcuaucu uaaaggccau uaaacagaau      600

gaagagaagc uggcaaacua ucagagugug ggggaguacc uuuauaaaga auacuuucag      660

aaguucaagg aaaauuccaa agaauuuacu aacgucagga auaagaaaga gucuuacgaa      720

cgguguaucg cucagagcuu cuugaaggac gagcugaaac uaauauuuaa gaagcaacgu      780

gaguucggau uuuccuucuc uaaaaaguuc gaagaggaag ugcucagugu ugccuuuuau      840

aaacgcgcgc ugaaggauuu cagccaccuu gugggcaacu gcucauuuuu cacggacgag      900

aaaagagccc cuaaaaauuc cccacuggca uuuauguucg ucgcuuugac caggauuauc      960

aaccuccuga auaacuuaaa gaacacagaa ggcauucugu acacuaaaga ugaccucaau     1020

gcccuucuga acgagguguu gaagaauggg acccuaacau auaaacagac caagaaacug     1080

cucggacugu cagacgauua cgaguuuaag ggugaaaagg gcacuuauuu caucgaguuu     1140

aaaaaguaca aagaguucau caaggcucuu ggggagcaua accugagcca ggacgauuug     1200

aaugaaauug ccaaagacau aacacucauc aaggaugaga uuaaacugaa gaaggcauua     1260

gccaaauaug accugaacca aaaucagauc gauagucuau cuaagcucga guuuaaagac     1320

caccugaaca uauccuucaa ggcucuuaaa uugguaacuc cccugaugcu cgaaggaaag     1380

aaguacgaug aggcguguaa ugaacugaac cuuaaaguug caauuaauga ggacaagaaa     1440

gauuuucugc cugccuucaa cgagacguac uauaaggacg aagugacuaa uccagucgug     1500

uugcgggcua ucaaagagua ccgaaaggug cucaacgccc uguuaaagaa auauggcaag     1560

guccacaaaa ucaauauuga acuggcacgc gagguuggua agaaccauag ccagagagcc     1620

aaaaucgaaa aggagcagaa cgagaauuac aaggcuaaaa aggacgccga acuagagugc     1680

gaaaaacucg ggcugaagau uaacucgaaa aauauacuua aguugaggcu guucaaagag     1740

caaaaggaau uuugugcaua uucuggagag aagaucaaaa uuuccgaucu ccaggacgag     1800

aagaugcugg aaaucgauca caucuacccc uauucacgga guuucgacga uagcuacaug     1860

aacaaagugu uaguauuuac caagcagaau caagagaaac ugaaccagac accuuucgaa     1920

gcguuuggca augacuccgc uaaguggcag aagauugagg ugcuugccaa aaacuugcca     1980

acuaagaaac agaagcgcau acucgauaaa aauuacaagg acaaggaaca aaagaacuuc     2040

aaggauagaa aucugaacga caccagguau aucgcacguc uaguccugaa uuacacaaaa     2100

gauuaucucg acuuucugcc gcuuucugau gacgagaaca ccaaguugaa ugacacgcag     2160

aaaggcagca aggugcaugu ugaggccaag ucagggaugc ugaccagugc uuuacggcac     2220

acauggggau ucuccgccaa agaucgaaac aaccaccucc aucacgcaau ugacgcugug     2280

aucauugccu acgcgaauaa cagcaucguc aaggccuuuu cugauuucaa aaaggaacag     2340

gagucaaaua gugcugaacu guaugccaaa aagauaagcg agcuugacua caagaacaaa     2400

agaaaguuuu ucgagcccuu uuccgguuuc aggcagaaag ugcuggauaa gaucgacgaa     2460

auuuucguau cuaaaccuga gcgcaagaaa ccaucgggcg cauugcauga agagaccuuu     2520

cggaaggaag aggaguucua ucaaagcuac gggggaaagg aaggcguucu caaagcucug     2580

gagcuaggua agaucagaaa agugaauggg aagauuguca aaaacggcga uauguuuagg     2640

guggacaucu ucaagcacaa gaaaacuaau aaguuuuacg ccguccccau auauacaaug     2700

gauuucgcac ugaaagugcu cccuaacaag gcgguugccc gcuccaaaaa gggagaaauu     2760

aaggacugga uccugaugga ugagaauuac gaauuuugcu ucucacuuua uaaagacagu     2820

uugauucuga uccagaccaa ggauaugcag gagccagagu uuguguacua uaacgcuuuc     2880

acgucuagca ccguauccuu aauagucuca aaacaugaca auaaguuuga aacacucagc     2940

aaaaaccaga agauccuguu caagaaugcc aacgagaaag aagugauugc aaagucuauc     3000

ggcauucaaa aucuuaaagu uuucgagaag uacaucgugu ccgcucuggg ggaagucacu     3060

aaagccgagu uucgacagcg ggaggacuuc aagaaaaagc gucccgcagc caccaagaaa     3120

gcuggacagg cgaagaaaaa gaaauaagga cuagugucca ccugucccuc cugggcugcu     3180

ggauugucuc guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3300


<210>  3679
<211>  3711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3679
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaagc gaccugguuc ucggacuuga uauugguaua      180

ggcuccgugg ggguaggaau ccugaacaag gugaccggcg agauuaucca uaaaaauucu      240

aggauuuucc cagccgcgca ggcugaaaac aauuuggucc ggcgcacaaa ccgacaaggu      300

cguagauuag ccaggcggaa gaagcaccgc agagugaggc ugaaucggcu auuugaggaa      360

ucagggcuca ucacugacuu cacgaaaaua aguaucaacc ugaauccgua ccagcuucgc      420

guuaagggac ugaccgauga guugagcaac gaagaacucu uuauugcacu gaaaaauaug      480

gugaagcauc gaggcaucuc cuaucuggac gaugccucug acgauggcaa cucgucaguc      540

ggggacuacg cucagauugu gaaagagaau agcaagcagc ucgaaacaaa gacucccgga      600

caaauccagu uagagagaua ucagaccuac ggucagcuga ggggcgauuu cacaguagaa      660

aaagacggga agaaacaccg gcuuauaaac guuuuuccua ccagugcaua ucguuccgag      720

gccuugcgca uucugcaaac ucagcaggag uucaauccac agaucacaga ugaauuuauu      780

aacagauacc uagagauccu caccggaaag aggaaauauu accauggccc cgguaaugaa      840

aagucucgga cggacuacgg gcgauaucgc acuagcggag agacccugga uaacaucuuc      900

ggcauucuua uaggcaaaug cacauuuuac ccugacgagu ucagagcugc caaggcgucc      960

uauacugcac aagaguuuaa ccuguugaau gaccucaaca aucugaccgu gccaacagag     1020

accaagaaau uaucaaagga acagaaaaac cagaucauua auuacgucaa gaacgagaaa     1080

gccauggggc ccgcuaagcu guucaaguau aucgccaaac uccuuaguug ugauguggca     1140

gacauaaagg gauacaggau ugauaaaagc gguaaggcug aaauccacac guuugaggcc     1200

uaucggaaaa ugaagacucu ggaaacauug gacaucgagc aaauggaucg cgagacccua     1260

gacaagcugg cguacgugcu cacucugaau acagaaagag agggcauuca ggaagcccuu     1320

gagcacgagu ucgcagaugg gucuuucucc cagaaacagg ucgacgagcu gguucaauuu     1380

aggaaggcua acucaagcau cuucggaaaa ggcuggcaua auuuuagugu gaaguugaug     1440

auggagcuca uuccugaacu guacgagacc ucugaagaac agaugacgau auuaacccgu     1500

cuggguaaac agaagacaac uuccagcucg aacaagacca aauauaucga ugagaagcua     1560

cucacagaag agauuuacaa uccaguagug gccaaaucug uccggcaggc uaucaagauc     1620

gugaacgccg caauuaaaga auauggggac uucgauaaua uaguuaucga gauggcccga     1680

gaaacuaacg aggacgacga gaagaaagcu auucaaaaga uccagaaggc gaauaaagau     1740

gaaaaggacg cagccaugcu gaaagcugcc aaccaguaca acggcaaggc agagcuuccg     1800

cacuccgugu uucauggaca caaacaguug gccaccaaga uuagacugug gcaccaacag     1860

ggcgaaaggu gccucuauac agggaagacc aucucaauac augaucugau caauaacagu     1920

aaucaguucg aggucgacca cauucuuccc cugagcauca cuuuugauga cuccuuggcu     1980

aacaaagugc ucguauacgc cacggcaaau caggaaaagg gacaacgcac accuuaucag     2040

gcgcuggauu cuauggacga ugcuuggagc uuccgggagu uaaaagccuu uguuagagag     2100

ucaaagaccc ugaguaacaa aaagaaggaa uaccuacuca cugaggaaga cauuuccaaa     2160

uucgauguga ggaagaaauu uaucgagcgc aaucuggucg acacccgaua cgcaagccgg     2220

gugguccuua acgccuugca ggaacauuuc cgugcucaca agauagauac aaaagugucu     2280

guugugagag gucaauuuac cucacagcug aggcgccauu ggggcauuga gaagacucgg     2340

gacacauauc accaccaugc cguagacgca cucaucauug cugccaguag ccagcugaau     2400

uuauggaaga aacagaagaa cacgcugguc uccuacucug aggaucaacu uuuggacauc     2460

gaaaccgggg agcucauauc ggaugacgaa uauaaagaga gcguguucaa ggcgccauac     2520

cagcacuucg uugauacucu gaaauccaag gaguuugaag acucaauccu auucaguuau     2580

cagguggauu cuaaauuuaa uagaaagauu agcgacgcca caaucuacgc uaccaggcag     2640

gcaaaggucg gaaaagauaa ggccgacgag acauaugugc ugggcaaaau uaaggauauc     2700

uacacucaag acgguuacga ugcuuucaug aaaauauaua agaaggacaa auccaaguuu     2760

cucauguacc gacaugaccc ccagaccuuc gaaaaaguaa uugagccuau ccuggaaaac     2820

uauccaaaua agcagaucaa cgagaaaggg aaggaggugc ccuguaaucc guuucuuaag     2880

uacaaagagg aacacggcua uauucgcaag uacagcaaaa agggaaacgg cccugaaauc     2940

aaaucauuga aguauuacga uucuaagcug gggaaccaca uugacauaac gcccaaagau     3000

uccaauaaca agguugucuu acagagugug agcccauggc gggccgacgu guacuucaau     3060

aaaaccacag gaaaguauga gauccucggu cugaaauacg cagaucuuca auuugaaaag     3120

ggcacuggga ccuauaagau uucucaggag aaauacaacg acaucaagaa aaaggaggga     3180

gucgauuccg acucagaguu caaauuuaca cuguauaaga augauuugcu ccugguuaaa     3240

gacacugaaa ccaaggaaca gcaacuauuc agauuucuga guaggacgau gccuaagcag     3300

aaacauuacg uggagcucaa gcccuaugau aaacagaagu ucgaaggcgg ugaggcgcug     3360

auuaaagugc uugggaacgu agccaauagc ggccagugca agaagggauu gggcaaaucg     3420

aacaucucca uauacaaggu ccguacagac gugcugggga aucaacacau cauuaaaaac     3480

gagggagaca agccaaaauu agauuucaag cgccccgcug ccaccaagaa agcaggccag     3540

gcuaagaaaa agaaauaagg acuagugucc accugucccu ccugggcugc uggauugucu     3600

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              3711


<210>  3680
<211>  4269
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3680
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaacc caguucgagg gauuuacaaa ccuguaccaa      180

guuagcaaga cucucagguu cgaacuuauu ccacagggua aaacgcugaa gcauauacag      240

gagcagggcu uuaucgaaga ggacaaggcc cggaaugauc acuauaaaga auugaagccg      300

auuaucgacc gcauuuacaa aaccuaugcg gaucaaugcu uacagcuggu gcagcuagac      360

ugggagaacc ucuccgcugc caucgauucu uaccgaaagg agaaaacaga agagacucgu      420

aaugcacuga uagaggaaca ggccaccuau agaaacgcua uccaugacua cuucauuggg      480

aggacagaua aucuuaccga cgcaaucaac aagcggcacg ccgagauuua uaagggacug      540

uuuaaagcug aauuguucaa uggcaaggua cucaaacaac uggguacugu gacaaccacg      600

gagcaugaaa acgcccugcu ccgcucauuu gauaaguuca cuaccuacuu uaguggguuc      660

uacgagaaua ggaaaaacgu cuuuagcgcg gaagacaucu ccacagcaau accccacaga      720

auugugcagg auaauuuccc uaaguuuaaa gagaacuguc acaucuucac ucgguuaauu      780

accgccguuc caucucugcg cgagcauuuc gaaaauguga agaaggcuau cggaaucuuu      840

gucucgacau caauugagga aguguucagc uuucccuucu auaaccagcu uuugacccag      900

acgcaaauag accuguacaa ucagcuacuc ggcggcauca gucgagaggc cgggacugag      960

aaaauuaagg gacugaacga aguacuuaac cuggcaaucc agaaaaauga ugagacagcu     1020

cacauaauug ccuccuugcc ucauagauuu aucccacucu ucaagcaaau ccugucugac     1080

aggaacaccc uuagcuuuau ucuggaagag uucaaauccg acgaagaggu uauccaguca     1140

uuuugcaagu auaagacucu ccuucggaau gaaaacgugc uggagacagc ggaggccuug     1200

uucaaugaac uaaacaguau ugaucugacc cacauauuua ucagccacaa aaagcucgag     1260

acgauuucuu ccgcacugug ugaccauugg gauacccuuc guaaugcucu guacgaacgc     1320

agaaucucag aguugacagg uaaaaucacu aagagcgcca aagagaaggu ccagaggagu     1380

cucaagcacg aagacauuaa ccugcaggag auaaucucug cugccggcaa agaauuaucc     1440

gaggcauuca agcagaaaac cagcgaaauu cugucgcaug cccacgcugc gcuagaucaa     1500

ccccucccua caacucugaa gaaacaggag gagaaggaaa uccuuaaguc ucaguuggac     1560

ucccugcucg ggcuguauca ucuucuggau ugguuugcag uggacgaguc aaaugaagug     1620

gauccagagu ucagugcccg guugaccgga auuaaacucg aaauggagcc gagccugucc     1680

uucuacaaca aggcucgaaa uuaugccacu aaaaagcccu acucugucga gaaauuuaag     1740

uuaaacuucc aaaugccuac ccuggcaagc ggcugggacg uuaauaaaga aaagaacaau     1800

ggugccaucc uauuugugaa gaacgggcuc uacuaucugg gaauaaugcc aaaacagaag     1860

ggccgcuaca aagcucuuuc auucgagccc acugaaaaga cgagugaggg cuuugauaaa     1920

auguauuacg acuauuuccc ugacgccgca aagaugaucc caaagugcuc cacacaguug     1980

aaagcgguaa ccgcucacuu ucagacucac accacaccca uucugcucag caacaauuuc     2040

aucgaaccgc uggagauuac caaggagauc uacgauuuaa acaauccuga aaaagagccc     2100

aagaaauuuc aaacugccua ugcaaagaag acaggggacc agaaaggaua cagagaagcc     2160

cuguguaagu ggauagauuu cacgagggac uuucuuucua aauacaccaa gacuacauca     2220

auugauuuga guagccuccg gccauccucu caguauaaag accuggguga guacuaugcu     2280

gagcuaaacc cucugcucua ccauaucucg uuccagcgca uugccgaaaa ggagaucaug     2340

gaugcagugg aaaccggcaa gcuguaucuu uuucaaauau acaauaaaga cuucgcuaag     2400

gggcaccaug gaaaacccaa cuugcacaca cuguauugga cuggcuuauu cagcccagag     2460

aaucucgcca agaccuccau caaacugaac ggucaggcgg aauuauuuua cagacccaag     2520

ucaaggauga aacguauggc ccaccggcug ggggagaaga uguugaauaa gaaacucaag     2580

gaucagaaaa cgccuauucc agacacccug uaccaagagc uauaugauua cgucaaccau     2640

cgacugaguc acgaccucuc ugaugaagcu agagcacugc uuccgaaugu gaucacaaag     2700

gagguuagcc augaaauuau caaagacagg cgcuucacuu ccgacaaguu uuucuuucac     2760

gugcccauaa ccuugaacua ucaggccgcu aauucaccua gcaaguucaa ccagcggguc     2820

aaugccuacc ugaaagagca uccagaaaca cccauuaucg gcaucgauag aggagagagg     2880

aacuuaauuu auaucacugu gauugacucu accggcaaga uacucgagca gcgcucccug     2940

aacacgaucc aacaguuuga uuaccagaaa aagcuggaca aucgagaaaa agagcgggua     3000

gcagcgcguc aggccuggag uguugugggg acaauuaagg aucuuaagca aggauaucua     3060

agccagguca uccacgaaau uguggaccuc augauccacu accaggcugu cgugguucug     3120

gagaacuuga auuucgguuu uaaaucuaag agaaccggca uagccgaaaa agcaguguac     3180

cagcaauucg agaagaugcu gaucgauaaa uuaaacugcc ucguacugaa ggacuauccu     3240

gcugagaagg ucgggggagu gcuuaaucca uaccagcuga cugaucaguu uacauccuuc     3300

gccaaaaugg gcacccaauc agguuucuug uuuuauguuc ccgcaccgua cacaaguaag     3360

auugacccuc ucaccggguu cguggaucca uuugucugga aaacaaucaa gaaccaugaa     3420

agcaggaaac acuuccugga gggcuuugac uuccuacauu augaugugaa gacgggagac     3480

uuuauucugc acuucaagau gaaucgcaac cuuucguuuc agcggggccu ccccggguuc     3540

augccugccu gggacaucgu auuugagaaa aaugaaaccc aguucgaugc uaagggaacu     3600

cccuuuauag cggguaaaag aauugugcca guuaucgaga accauagguu cacaggccga     3660

uaccgcgacc uguauccugc caaugaauug aucgcacugu uagaggaaaa ggggauuguc     3720

uuccgggaug gauccaacau acuccccaaa cugcuugaga augacgauuc ucacgcuauc     3780

gacaccaugg uggcccugau uagaagcgug uugcagauga ggaacucaaa ugccgcuaca     3840

ggcgaagauu acaucaacag uccaguccgu gaccucaacg guguuuguuu ugauucccgc     3900

uuccaaaauc cggaguggcc uauggacgca gaugccaacg gggcguacca cauugcucug     3960

aagggccagc uacugcuuaa ucaucucaaa gagucuaagg accugaaguu gcagaacgga     4020

aucagcaauc aggacugguu agccuauaua caagaacugc ggaacaaaag acccgcagcc     4080

acuaagaaag cuggccaggc aaagaaaaag aaguaaggac uaguguccac cugucccucc     4140

ugggcugcug gauugucucg uuuuccugcc aaauaaacag gaucagcgcu uuacagaucu     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaagaauu                                                             4269


<210>  3681
<211>  4248
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3681
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcaagc auuuaccagg aguucguuaa caaguauucc      180

cugucuaaaa cccucagguu ugaacuuaua ccacaaggaa agacacugga gaauaucaag      240

gcccgggguu ugauuuuaga cgaugaaaaa cgcgcgaagg acuacaaaaa ggcuaaacag      300

aucauugaua aguaucauca guucuuuauc gaggaaauac ugucaagugu gugcaucagc      360

gaggaccuac uccagaacua cuccgaugua uauuucaagc ugaaaaaguc ugacgaugac      420

aaucuucaaa aagauuuuaa gucggccaaa gacacuauua agaaacagau cucagaguac      480

auuaaggaua gcgaaaaguu caaaaaccug uuuaaucaga acuugaucga cgcaaagaaa      540

ggccaggaga gugaucucau acuguggcug aagcaaucca aagacaaugg gauugaacuc      600

uucaaggcca acucugacau cacggauauu gacgaggcuu uagagaucau caagagcuuu      660

aaaggaugga ccacauauuu caagggcuuu cacgaaaauc guaaaaacgu guacuccuca      720

aaugauauuc cgacaaguau aaucuaccga auugucgacg auaaccugcc caaguuccuu      780

gagaauaaag caaaguauga gagcuugaag gacaaagccc cugaggcuau caacuacgaa      840

cagauaaaga aagaucuggc cgaggagcua accuuugaca uugauuauaa gacaucugaa      900

gugaaucaga gaguuuucuc ccucgacgag guguucgaaa ucgcgaacuu uaacaauuac      960

cugaaccagu cagguaucac caaauucaau acuauuaucg ggggaaaguu ugucaacggc     1020

gagaauacaa agaggaaagg cauuaacgaa uauauaaauc uuuacagcca acagaucaac     1080

gauaagaccc ugaaaaagua uaaaaugagu guguuguuca agcagauucu cucugacacg     1140

gaguccaaga gcuuuguaau cgacaaacug gaggaugacu cggauguugu gacuaccaug     1200

caaucuuucu acgaacagau cgcagccuuu aagacagucg aggaaaaauc cauuaaggag     1260

acuuuaucac uccuguucga cgaucuuaaa gcucagaagc uggacuugag uaaaauauac     1320

uuuaagaaug auaagagccu aaccgaccug ucccagcaag uguucgauga cuauucugug     1380

aucgggacag ccguccucga auacauuacc cagcagaucg cacccaaaaa ccuggauaau     1440

ccaagcaaga aagagcagga gcuuauugcu aagaaaacgg aaaaggccaa guaucuguca     1500

uuggagacua ucaaacucgc gcuggaagag uuuaacaagc aucgggacau agauaaacaa     1560

ugucgcuucg aggaaaucuu agccaauuuu gcagcuauuc cuaugaucuu cgacgagauu     1620

gcccagaaca aggacaaucu ggcucagauc aguauaaaau accagaacca aggaaagaag     1680

gaucuacucc aggccuccgc agaagacgau guuaaagcca uuaaggaccu gcuugaucag     1740

acaaacaauu ugcugcacaa acucaagauc uuccauauua gccagucuga ggacaaagcu     1800

aacauccugg auaaggacga acacuuuuau cuuguguucg aggagugcua cuuugaacug     1860

gcgaauauag uaccauugua uaacaaaauc agaaauuaca uuacccaaaa gcccuacuca     1920

gaugagaagu ucaaacucaa cuuugaaaau aguacucugg caaacgguug ggacaagaau     1980

aaagagccug auaacacagc caucuuauuc auuaaggacg auaaauauua ccugggcgug     2040

augaauaaga agaacaauaa aaucuuugac gacaaggcua uaaaagaaaa caagggggag     2100

ggauauaaaa agauugucua caagcuacuc ccaggcgcca auaaaaugcu gccgaaggug     2160

uucuuuagcg caaaauccau caaguucuau aaccccucug aggauauccu uaggauucgg     2220

aaccacucga cccauacgaa aaaugguagc ccucagaagg gguacgaaaa guuugaguuc     2280

aacaucgaag acugucgcaa auuuauugau uucuauaagc aguccauauc aaaacaccca     2340

gaguggaagg acuucggauu ucgauucagu gauacccaaa gauacaauuc uaucgacgaa     2400

uuuuacaggg agguugagaa ccagggcuau aaauugacau ucgaaaauau uagcgagucc     2460

uacaucgauu caguggucaa ccagggcaag cuguaucucu uucagauuua caauaaggac     2520

uucagcgccu auucuaaagg gcggcccaac cugcauacuu uauacuggaa ggcucuguuu     2580

gaugaacgua aucuucaaga cgugguauau aaauugaacg gagaggccga acucuucuac     2640

cgcaagcagu ccaucccuaa aaagauaacc cacccagcaa aagaagcgau cgcuaauaag     2700

aacaaggaua aucccaaaaa ggagaguguu uuugaauacg accugauuaa agacaagaga     2760

uucacagagg auaaauuuuu cuuccacugc ccgaucacua uuaacuuuaa gagcucuggu     2820

gccaauaagu ucaacgacga aaucaaccua cugcucaaag agaaggcaaa ugaugugcau     2880

auacugucca uugacagggg cgagcggcac cuugccuauu acaccuuggu cgaugggaaa     2940

ggaaacauca ucaagcagga cacauuuaau auuauaggca acgaucgaau gaaaaccaau     3000

uaucaugaca agcuggcugc caucgaaaag gaucgcgacu cagcaagaaa agauuggaag     3060

aaaauuaaca auaucaagga gaugaaagaa gguuacuuaa gucagguggu ccacgagauu     3120

gcuaagcucg ugaucgaaua uaacgccaua guuguguucg aggaccugaa uuuuggguuc     3180

aagaggggcc gguuuaaagu agagaagcaa gucuaccaga aacuugaaaa gaugcugauc     3240

gagaaauuga acuaucucgu guucaaggau aaugaauuug acaaaacugg aggcguucug     3300

cgcgcguacc agcuaacggc cccuuucgag acauuuaaga agauggggaa acagaccgga     3360

auuaucuacu augugcccgc ugguuucacu agcaagauuu guccagucac cggcuuugug     3420

aaccaacugu acccuaaaua ugaaucggua uccaagucuc aggaguucuu cagcaaauuu     3480

gacaagaucu gcuacaaucu cgauaagggg uauuucgagu uuucauucga cuacaaaaac     3540

uuuggagaua aggcagccaa aggcaagugg acaauagcua guuucgguuc cagacugauu     3600

aauuuuagga acucugacaa aaaucauaac ugggauaccc gugaagugua ucccacuaag     3660

gagcuugaaa aguugcugaa agacuacagc aucgaguacg ggcacggcga guguaucaag     3720

gccgcaauuu gcggagaauc cgauaaaaag uucuuugcga aauuaacauc aguucucaac     3780

acgauccugc agaugcggaa uagcaagacc ggcacugagc uugacuaucu gauaagucca     3840

gucgccgaug ugaacgggaa uuucuuugac ucucgacaag cucccaagaa caugccucag     3900

gaugccgacg caaauggagc uuaccacauu ggucuaaaag gccucaugcu guuggggaga     3960

aucaagaaca aucaggaagg caaaaagcug aacuuaguga uuaaaaauga ggaauauuuc     4020

gaguuugucc agaacaggaa uaacaagcgc ccagccgcaa cuaaaaaggc cggacaagcu     4080

aagaaaaaga aauaaggacu aguguccacc ugucccuccu gggcugcugg auugucucgu     4140

uuuccugcca aauaaacagg aucagcgcuu uacagaucua aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  4248


<210>  3682
<211>  4433
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3682
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaga caagaaauac agcauuggac uggauauagg uacaaacucc guuggcuggg      180

ccgugaucac agacgaguau aagguaccau cuaagaaauu caaggugcuc gggaauacug      240

auaggcauuc aauuaaaaag aaccuuaucg gagcgcuguu guuugacagu ggcgaaacgg      300

cugaggccac ccgguuaaaa cgcacagcac gacguagaua cacuaggcgg aagaaucgca      360

uuugcuaucu gcaggaaauc uucagcaacg agauggccaa ggucgaugac uccuuuuucc      420

acagacuaga agagucuuuu cucguggagg aagauaaaaa gcaugagagg cacccgauau      480

ucgguaauau cguugacgaa guggcuuacc augagaaaua ucccaccauu uaccaucugc      540

ggaagaaacu ugucgauucg acagacaagg cagaucugcg cuugaucuau cucgcccugg      600

cucacaugau uaaauuucga gggcacuucc ugaucgaggg agaccucaac ccugauaauu      660

cagacgugga caaguuauuu auacaacugg uacagaccua caaccagcuu uucgaagaga      720

auccaauuaa cgccagcggc guugaugcga aggcaaucuu gagugccaga cuguccaaau      780

cuaggcggcu agaaaaucuc auugcucagc ugcccggcga gaagaaaaac gggcuuuuug      840

gaaaucugau cgccuugagc cucggucuga cuccuaacuu caaguccaau uuugacuuag      900

cagaagaugc uaaacugcaa cucucaaagg acacauacga ugacgaucuu gacaaccugu      960

uggcccagau cggcgaucag uaugcggacc uauuccuggc cgcaaagaac cucagugaug     1020

cuauucugcu uagcgacaua cugcguguga auaccgagau cacgaaagcc ccauugucug     1080

cuuccaugau uaagcgcuac gacgagcacc aucaggaucu cacucuguua aaagcccugg     1140

ucagacaaca gcuacccgaa aaguauaaag agaucuucuu ugaccaguca aagaacgggu     1200

acgcaggaua uauagauggc ggugccagcc aggaagaguu cuacaaguuu auuaaaccua     1260

uccucgaaaa gauggacggg accgaggagc ugcuugugaa auugaauagg gaagaucugc     1320

uccggaagca acgaacauuc gacaacggaa guaucccaca ccagauucac cugggcgagc     1380

uucaugcuau ccugcgcaga caggaagauu uuuauccguu cuugaaagac aauagggaga     1440

agauugaaaa gauacucacu uuucggaucc ccuacuacgu gggcccucug gcgcgcggga     1500

acucuagauu cgcauggaug accaggaaau ccgaggagac aauuacccca uggaauuuug     1560

aagaggucgu ugauaaggga gccagcgcuc aaucguucau cgaacguaug acgaacuuug     1620

acaaaaauuu acccaacgag aaggugcugc cuaaacacuc ucuacucuau gaguacuuca     1680

cuguauauaa ugaacugaca aaggugaaau acgucaccga ggguaugcgg aagccagccu     1740

uucuuuccgg cgaacagaag aaagcaaucg uggauuugcu guucaagacu aaccgaaaag     1800

uuacagugaa gcagcucaaa gaggacuauu ucaagaagau ugaauguuuu gauucagucg     1860

agauaagugg gguggaggac agauucaaug ccagccuggg aaccuaccau gacuuacuga     1920

aaaucauuaa ggauaaagac uuucuugaua acgaagagaa ugaagacauc uuggaggaua     1980

uuguacucac gcugacccua uucgaagaca gggagaugau cgaggaacgc cugaagacau     2040

augcucaccu cuuugaugac aaaguuauga agcagcugaa gcggagaagg uacacuggcu     2100

ggggucgccu uucccgaaaa uugauaaacg ggauccggga uaagcaaucu ggcaaaacca     2160

uucuggacuu cuuaaagagc gauggauuug ccaaccguaa uuucaugcag cucauccaug     2220

acgauucacu gacauuuaaa gaggacauuc agaaggcaca ggugaguggc caaggggacu     2280

cccuucacga acacaucgcg aaccuggcug gaagccccgc cauaaagaaa gguauuuugc     2340

agacugucaa gguggucgau gagcucguga aaguuauggg cagacauaag ccggaaaaua     2400

ucgugauuga gauggcaagg gagaaccaga ccacacagaa agggcaaaag aacucucgcg     2460

aacggaugaa aagaaucgag gaaggaauaa aggagcuggg cucacagauc cuaaaggagc     2520

acccuguaga aaauacccag cugcagaaug agaaacucua ccuguauuac cuucaaaacg     2580

guagggacau guaugucgau caggaauugg acauuaaucg acugagugau uacgacgugg     2640

aucauaucgu uccccagagc uucuuaaagg acgauuccau ugacaacaaa gugcucacuc     2700

gcucugauaa gaaucggggg aaaucggaca acgucccaag cgaggaagug guaaagaaga     2760

ugaaaaauua uuggagacaa cugcuuaacg ccaagcugau cacgcagagg aaauuugaca     2820

aucuaacaaa ggcugagcgu ggcggacucu ccgagcugga uaaagccggc uucauaaagc     2880

gccaguuggu ggaaacccgg cagauuacua agcacguugc acaaauccug gacucaagaa     2940

ugaacaccaa auacgaugag aacgacaagu uaaucaggga agucaaagug auuacacuca     3000

agaguaaacu ggugucugau uuucgaaagg acuuccaguu cuauaagguc cgggagauca     3060

auaacuacca ccaugcucac gaugccuacc uuaaugcggu uguggggacc gcccugauua     3120

aaaaguaucc uaaauuggaa agcgaguuug uguacggaga cuauaaggua uacgaugucc     3180

gcaaaaugau agcuaagucc gagcaggaaa ucgguaaggc aacugccaaa uauuucuuuu     3240

acucaaacau uaugaauuuc uuuaagacag agaucacgcu cgcuaacggc gaaauuagaa     3300

aaaggccccu gaucgagacc aauggggaaa cuggagagau agugugggac aagggccggg     3360

auuucgccac aguucgaaaa gugcuaagca ugccacaggu caacaucgug aagaaaaccg     3420

agguucaaac agguggguuu ucuaaggaau ccauucugcc caagagaaau agugacaaac     3480

uuaucgcacg caagaaagau ugggacccua agaaauaugg cggauucgac agcccaacug     3540

uggcguacuc uguacucguc guggccaagg uggagaaggg caaauccaag aaacugaagu     3600

cagucaaaga auugcugggg auuaccauca uggagaggag uagcuuugag aagaacccga     3660

uagauuucuu agaagcuaag ggauacaaag agguuaagaa agaccucauu aucaagcugc     3720

ccaaauauuc gcuuuuugaa cuggagaaug gccguaagcg gauguuggcc uccgcuggug     3780

aacuccagaa ggggaacgag cuggcacuac cuucuaaaua cgugaauuuc cuguaucuug     3840

ccagccauua cgagaagcuc aaaggaucac cagaagauaa cgagcagaag cagcuguucg     3900

uagaacaaca caaacauuau uuggacgaga ucauugaaca gauaagugag uuuuccaaga     3960

gagugaucuu agcagaugcc aaucuggaca aaguccucuc ugcuuacaac aagcacaggg     4020

auaagcccau ucgcgagcag gcggaaaaca ucauucaccu guucacgcuu accaaucugg     4080

gcgccccugc agcuuuuaaa uauuucgaca caacuaucga ucgaaagaga uacaccagca     4140

caaaagaggu guuggacgcc acucuaauac aucaauccau caccggucug uacgaaacgc     4200

ggauugaucu cucacagcug ggcggggaca agaggccagc cgcuaccaaa aaggcaggac     4260

aggccaagaa aaagaaauaa ggacuagugu ccaccugucc cuccugggcu gcuggauugu     4320

cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4433


<210>  3683
<211>  5216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3683
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa cuucaagauu cugccaauag ccaucgaccu cggaguuaaa aauaccggug      180

uguuuagcgc guucuaccag aagggcacau cccuugagag gcuggauaac aagaauggga      240

aaguauauga auugucuaag gacucauaca cuuuacugau gaacaaucgg acggcucgcc      300

gacaucaacg uagaggaauu gauaggaaac agcuagugaa gcggcucuuu aaacugaucu      360

ggaccgagca gcuuaaccug gaaugggaca aggauacaca gcaagccauu aguuucuugu      420

uuaaucgcag aggcuucagc uuuaucacug acgguuauuc cccggaguac cucaacauag      480

uccccgaaca ggugaaggca auccugaugg auauuuucga cgauuauaau ggggaggacg      540

aucuggacuc uuaccucaaa uuagccaccg agcaggaauc gaagaucuca gagauuuaua      600

acaaacugau gcagaagauc cuugaauuua aauugaugaa gcugugcaca gauauaaaag      660

acgacaaggu uagcaccaag acucuaaaag agauuacaag uuacgaguuc gaacuccugg      720

cugauuaccu ugcaaauuau uccgagucuc ugaagaccca aaaauuuagc uacacggaca      780

agcagggaaa cuugaaagaa cucuccuauu accaccauga uaaguauaau auccaggagu      840

uccugaagag gcacgccacu auuaacgacc ggaucuuaga uacccugcuc acagacgauc      900

uugacaucug gaauuuuaac uucgaaaaau ucgauuuuga caagaacgag gagaaacugc      960

agaaucaaga agauaaggac cauauucagg cucacuugca ccauuucgug uuugccguca     1020

acaaaauaaa gucagagaug gcgaguggcg gccgccaccg aagccaguac uuccaagaaa     1080

ucacuaaugu gcuagacgag aacaaucauc aggaagggua ucugaagaac uuuugugaga     1140

aucuccacaa caaaaaguac ucuaaucugu ccguuaaaaa ccuuguaaau cugauuggaa     1200

acuugucaaa ucucgagcug aagccuuuaa gaaaauacuu caacgauaag auccacgcaa     1260

aggccgacca uugggaugaa cagaaauuua ccgagacaua uugccacugg auacugggug     1320

aauggagggu gggcgucaag gaccaggaua aaaaggacgg ggcuaaauac agcuauaagg     1380

aucuauguaa ugagcucaaa caaaagguga ccaaggccgg acugguggac uuccuuuugg     1440

aacuggaucc augccggacg auucccccuu accucgacaa caacaaucgu aaaccaccca     1500

agugucagag ucugauccuu aacccuaaau uucuggauaa ucaguaucca aacuggcagc     1560

aauacuugca ggagcucaag aaacugcagu cuauccagaa uuauuuagac uccuucgaga     1620

cugaucugaa gguccuaaag agcucgaaag accaaccgua cuuuguugaa uacaagucuu     1680

ccaaccagca gauugcauca ggccagcgcg acuauaaaga ucucgacgcu agaauccugc     1740

aauucauuuu ugauagggug aaggccagug acgagcuuuu gcugaaugaa auauacuucc     1800

aggcgaaaaa gcucaagcag aaagccagcu ccgagcugga gaaguuagaa ucuagcaaaa     1860

agcuggauga gguaaucgca aacucacaac uuagucagau uuugaaaucc cagcauacaa     1920

augguaucuu cgaacagggg accuuucucc accuggugug caaguauuac aagcaacggc     1980

agcgagcucg cgacagcaga cuauauauca ugcccgagua cagguaugau aaaaagcugc     2040

auaaauacaa caauacugga cgguucgacg augacaacca gcuccugaca uacuguaauc     2100

acaagccucg ccagaaaaga uaucaacuuu ugaacgaucu ggccggcguc uuacaggugu     2160

cuccaaauuu ucucaaggac aaaauuggcu cagaugacga ccuguucaua aguaaguggc     2220

uuguugaaca caucaggggg uuuaagaaag caugcgagga uagccugaag auucagaaag     2280

acaaccgugg auugcucaac cauaagauca auauugcccg gaacaccaaa gguaagugug     2340

agaaggaaau cuucaaucug auaugcaaaa ucgagggcuc cgaagauaag aaagggaacu     2400

acaagcacgg acuagcauau gagcugggcg ugcuccuguu uggugaaccc aaugaggccu     2460

cuaaaccuga guucgaccga aagauuaaga aauuuaacuc gaucuacagc uucgcucaga     2520

uucaacagau cgcguuugca gaaagaaagg ggaaugccaa cacgugugcu gucugcuccg     2580

ccgauaaugc acauaggaug cagcaaauaa aaauuaccga gccaguggaa gacaacaagg     2640

auaaaaucau ucuuucagcc aaggcucagc gcuugcccgc caucccgaca cggauaguag     2700

acggcgcagu uaagaaaaug gcgacuaucc uggcuaagaa uauuguggau gacaacuggc     2760

agaauaucaa acaggucuua agugccaagc accaacucca cauuccuauc auaaccgagu     2820

cuaacgcauu cgaauuugag cccgcccugg cugaugugaa aggaaagagc cuuaaggaca     2880

gaaggaaaaa ggcccuggag cgcauuuccc cagaaaauau cuucaaagac aagaacaacc     2940

gaaucaaaga guucgcaaag ggcauuucag cuuauagcgg ggccaaucua acagauggag     3000

acuuugaugg ugcgaaagaa gaacucgacc auaucauucc ucggucucac aagaaguacg     3060

gcacucugaa cgaugaggcc aauuugauau gcgucacccg uggggacaac aaaaauaagg     3120

gaaacagaau cuucugucug agggauuuag cugacaauua uaaacucaag caguuugaaa     3180

caaccgauga ccuggagauu gagaaaaaga ucgcagauac uauuugggac gccaacaaga     3240

aagauuucaa guuuggcaau uaccgcuccu ucaucaaccu uacgccccag gagcagaagg     3300

cuuuucggca ugcccuguuc uuggcagacg aaaauccaau aaaacaagcg gugaucagag     3360

ccauuaacaa uaggaaccga acauuuguua augguaccca gcgcuacuuc gcugaggugc     3420

ucgccaacaa uaucuaucug cgggcuaaaa aggagaaccu aaacacugac aagauuaguu     3480

uugauuacuu cgggaucccc accauaggca auggaagagg cauugcugaa aucaggcagc     3540

uguaugagaa aguagacagc gauauccagg ccuacgcaaa gggggacaaa ccucaagccu     3600

cuuauuccca ccuuauugau gcuaugcucg cguucugcau agccgcagac gaacaucgua     3660

acgauggauc aaucggucug gagauugaca agaauuacag uuuguaucca cuggauaaaa     3720

acacaggcga agucuuuacc aaggacaucu ucagccagau uaagaucacu gauaaugagu     3780

uuucggacaa aaaguuagug cgcaaaaagg cuauagaggg guucaacaca caccggcaga     3840

ugacgagaga uggaaucuac gccgaaaauu accucccgau ucugauccac aaagagcuua     3900

acgaaguuag gaagggcuau accuggaaaa auuccgagga aauuaagauc uuuaagggua     3960

aaaaguacga cauacagcaa cugaacaauu ugguguauug ucucaaauuc gucgacaagc     4020

ccauuucuau cgauauccag auuagcacuc uggaggagcu acgaaacauc cugacaacca     4080

auaacauagc cgcuacagca gaauacuauu acauuaaucu uaaaacucag aagcuccaug     4140

aguauuacau cgaaaacuac aacaccgccc ugggguauaa gaaauacuca aaggagaugg     4200

aguuuuugcg gaguuuagcg uaucgcuccg aaagagugaa aauuaagucu aucgacgaug     4260

uaaaacaagu gcuggacaag gauagcaauu ucauaauugg caagaucacg cucccuuuua     4320

aaaaggagug gcagaggcug uaccgggaau ggcagaacac cacaaucaaa gacgauuaug     4380

aguuccuuaa guccuuuuuc aauguuaaau caauuacuaa gcugcacaag aaaguccgaa     4440

aggacuuuag cuugccaauc aguaccaacg aaggaaaauu ccuagugaag agaaaaacau     4500

gggauaauaa cuucauuuac cagauacuga augacucuga uucccgcgcu gacggcacua     4560

agcccuuuau cccugccuuc gacauuagca aaaacgagau cguggaggca aucauugauu     4620

cguuuaccuc aaagaauaua uucuggcucc caaagaacau cgaacugcaa aaagucgaca     4680

auaagaacau uuuugccauc gauacgucua aaugguucga gguugaaaca cccagugacc     4740

uuagggauau ugggaucgcu accauacagu auaagaucga caauaacucc cguccgaaag     4800

ugcgggugaa guuagauuac guaauugacg augacagcaa gaucaauuac uuuaugaacc     4860

auucucugcu caaaagcaga uauccugaua aggucuugga gauucugaaa caguccacua     4920

ucauagaguu cgaaucaagu ggauuuaaca agacaauuaa agagaugcuc ggcaugaagc     4980

uggcagguau cuacaaugaa accagcaaca auaagaggcc agccgcgacu aaaaaggcug     5040

ggcaggccaa aaagaaaaag uaaggacuag uguccaccug ucccuccugg gcugcuggau     5100

ugucucguuu uccugccaaa uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa     5160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         5216


<210>  3684
<211>  3488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3684
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaggaacuac auucugggac ucgacauagg uaucacauca guuggcuaug      180

ggauuaucga uuacgagaca cgggacguga uugaugccgg aguacgccuu uucaaagaag      240

cgaaugugga gaacaaugaa ggccgacguu ccaagagagg ugcuaggcgg cugaagcgca      300

gaaggcggca ucgcauccag cgagucaaaa aguuguuauu ugacuauaac cugcuaacug      360

aucacucuga gcucucaggg auaaauccau acgaagccag agugaaagga cugagucaaa      420

aacuuagcga ggaggaguuc uccgcagccc uguugcaucu cgcuaaaagg cggggcguuc      480

acaacgugaa ugaagucgaa gaggacacgg gcaacgagcu gucuaccaag gaacagaucu      540

cgcguaauuc aaaggcacug gaggaaaaau auguggccga gcuccaguua gaacgccuga      600

agaaagaugg ggagguaaga ggaagcauua acagguuuaa gacaagugac uacguuaaag      660

aggcuaagca gcuuuugaaa gugcaaaagg ccuaucauca gcuggaucag uccuucaucg      720

acacuuacau ugaucuacuc gaaacccggc gaacauacua ugaggguccg ggcgaagggu      780

cucccuuugg auggaaggac aucaaagagu gguacgaaau gcugaugggc cacugcaccu      840

auuucccuga ggagcuucgc agcgucaagu acgcguauaa ugcagaucug uacaacgccu      900

ugaaugaccu caacaaucug gugauaacua gagacgaaaa cgagaaauua gaauauuacg      960

agaaguuuca gauuaucgaa aacguguuca aacaaaagaa gaaaccaaca cugaagcaga     1020

uugcuaaaga gauccucguc aaugaggaag auaucaaggg uuacaggguu accuccacgg     1080

ggaaacccga guuuacuaac cuuaaggugu aucacgacau uaaggauaua accgcccgga     1140

aagaaaucau ugagaaugca gagcuguugg accagaucgc uaagauacua acaauuuacc     1200

agucaaguga agauauccaa gaggaacuga cuaaccucaa uagcgagcug acccaggaag     1260

agaucgagca gauuucuaac cuuaaaggau auacaggcac ccauaaucug uccuugaagg     1320

ccaucaaccu cauucuggac gaauuauggc acacgaauga uaaccaaaua gcgaucuuca     1380

aucgccugaa acuuguaccu aagaaggugg accucucaca gcagaaagag auuccaacua     1440

cacuggucga ugacuuuauc cuuagccccg ugguuaagag aaguuucauc cagucuauua     1500

aagugauaaa cgccaucauu aagaaauacg gcuugccuaa ugauaucauu aucgaacugg     1560

caagggagaa gaacuccaaa gacgcucaaa agaugauaaa ugaaaugcag aagcguaacc     1620

ggcagaccaa cgagcgaauc gaggaaauua ucagaacuac agggaaagag aaugccaagu     1680

aucucauuga aaagaucaag cugcaugaca ugcaggaggg aaaaugucuu uacagccugg     1740

aagcuauacc auuggaggau cuccugaaca auccguuuaa cuaugagguc gaccacauua     1800

uccccagguc ggugucuuuc gauaauuccu ucaacaauaa gguauuaguu aagcaagaag     1860

agaacucaaa gaaggguaau cgcaccccuu uucaguaccu gaguagcucc gacucuaaaa     1920

uuagcuacga aacguucaag aaacacaucc uaaaccucgc caagggcaag gggcggauau     1980

caaaaaccaa gaaagaguau cugcuugagg aaagagauau caauagguuu agugugcaga     2040

aggacuucau uaaccgcaau uuggucgaua cacgauacgc aacucgggga cugaugaacc     2100

uccugcguuc cuauuuuaga gugaauaacu uagacgucaa agugaaaagc aucaacggcg     2160

guuucaccuc uuuucugagg cgcaagugga aauucaagaa agagcggaau aagggguaca     2220

aacaucacgc cgaagaugcu cuuauuaucg cgaacgcaga cuuuauauuc aaggagugga     2280

aaaaguugga uaaggccaaa aagguuaugg aaaaucagau guuugaggag aaacaagcug     2340

aaucaaugcc agagauugaa acagagcagg aauauaagga gaucuucauc acuccccauc     2400

agauuaaaca caucaaggac uuuaaggauu acaaauauag ucauagagug gacaagaaac     2460

cuaacaggga gcucauuaau gacacccugu acagcacacg aaaggaugac aaaggcaaca     2520

cccuaauagu aaauaaccug aauggacucu acgauaagga caacgauaag cugaaaaagc     2580

uaaucaauaa auccccagaa aaguugcuga uguaucacca cgacccccag acuuaccaaa     2640

aauuaaagcu cauuauggag caguauggcg augaaaagaa cccgcuguac aaauauuacg     2700

aggaaacggg gaauuaucuu acaaaguacu cuaaaaagga caacggaccu gucaucaaaa     2760

agauuaagua cuaugguaau aaacugaacg cccauuugga uaucaccgac gauuacccca     2820

acucgcgcaa uaaggugguu aaacucagcc ugaagccaua ucgguucgac guguaccuag     2880

auaacggcgu cuauaaauuc gugacuguaa agaaucugga cgugaucaaa aaggagaacu     2940

acuaugaggu uaauuccaag ugcuacgaag aggccaaaaa gcucaaaaag auaucaaacc     3000

aggcagaauu uauugcuagu uucuacaaua acgaccugau caaaauuaau ggggagcuuu     3060

auagagucau cggagugaac aaugauuugc ugaacaggau agaggugaau augauugaca     3120

ucaccuaccg ugaauauuua gagaacauga augauaagcg cccuccccgg aucauuaaga     3180

caauagccuc uaaaacccaa agcaucaaga aauacuccac ugacauucuc ggcaaccugu     3240

augaagucaa gagcaaaaag cacccacaga ucauuaagaa agguaagaga cccgcagcga     3300

ccaaaaaggc ugggcaggcc aaaaagaaga aauaaggacu aguguccacc ugucccuccu     3360

gggcugcugg auugucucgu uuuccugcca aauaaacagg aucagcgcuu uacagaucua     3420

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaagaauu                                                              3488


<210>  3685
<211>  4556
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3685
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcacu guucaacaag ugcauuauaa ucagcauuaa ucucgacuuu uccaacaaag      180

agaaguguau gaccaagcca uacucuaucg gacuugauau ugguacaaau ucaguuggcu      240

gggccgugau cacugacaac uauaaaguac cgaguaagaa aaugaaggug cuggggaaua      300

cgagcaaaaa guacauaaag aaaaacuugu uaggaguccu gcuauucgau uccggcauca      360

ccgcggaagg uaggcggcuc aagcgcacag cucgacguag auauacuagg cggcgcaaua      420

ggauucugua ccuucaggag aucuuuucua ccgaaauggc cacacuggac gaugcauucu      480

uucaaagauu ggacgauucg uuccucgugc ccgacgauaa acgggacuca aaguauccua      540

uuuuugggaa ccugguugag gaaaaagugu accaugauga guucccaacc aucuaucauc      600

ugcgcaagua ccucgccgac agcacuaaaa aggcugauuu acgacugguc uaccuugcau      660

uggcccacau gauaaaguau agaggacacu uucugauuga gggcgaguuc aauaguaaaa      720

acaaugacau ccagaagaac uuucaggacu uccuagauac auacaaugcu auuuuugagu      780

ccgaccucuc ucuggaaaac agcaaacagc uugaggagau cgugaaggau aaaaucucca      840

agcuggaaaa gaaagacagg auuuugaaac ucuuccccgg cgagaaaaau ucagggauau      900

ucagugaauu ucugaaguua aucguaggaa accaagccga uuuccggaaa ugcuuuaacc      960

uggacgagaa ggcgagccuc cauuucucua aggaauccua ugaugaggac cuugagaccc     1020

uguuggguua cauuggcgau gacuauucag auguuuuucu aaaagcaaag aaacuguacg     1080

acgccauccu ccugagcggg uuccuuacgg ugacugacaa ugaaaccgag gcuccucuga     1140

guucugccau gauaaagcgu uauaacgaac acaaagagga uuuggcacuc cugaaggaau     1200

acauucgcaa uaucuccuua aagacauaca acgaggucuu uaaagacgau acuaagaaug     1260

gauaugcugg cuacaucgac gguaaaacca accaggagga uuucuaugug uaccugaaga     1320

aucuacucgc cgaauuugag ggggcggacu auuuccugga aaagauugau agagaggacu     1380

uucuuaggaa gcagcggaca uucgauaacg gaagcauccc auaccagauu cacuugcaag     1440

aaaugcgagc cauacuggac aaacaggcaa aguuuuaucc cuuccucgcu aagaauaaag     1500

agcgcaucga gaagauucug accuucagaa ucccuuacua cgugggccca cuugccaggg     1560

gcaacucgga uuuugcuugg ucuauccgga aacgcaauga aaagauuacg ccguggaacu     1620

ucgaggacgu cauagauaaa gaauccucag ccgaggcauu uaucaauaga augacaaguu     1680

ucgaccugua uuugcccgag gaaaagguuc ucccuaagca uagccuguua uacgagacuu     1740

uuaacgugua uaaugaacug accaaaguaa gguucauugc cgaguccaug cgugacuacc     1800

aguuucuaga uucuaagcag aaaaaggaca ucgugcggcu cuauuucaaa gauaagcgaa     1860

aggucacuga caaagauauu aucgaauacc ugcacgcuau auauggguac gacggaaucg     1920

agcuuaaggg uauugagaaa caauuuaaca gcucauugag uacauaccau gaucugcuca     1980

acaucauuaa ugacaaggag uuccuggaug acuccagcaa cgaagcgauc auagaagaga     2040

uuauccacac cuuaacgauu uuugaagaua gagagaugau caaacagagg cugucuaagu     2100

ucgagaauau auuugacaag ucagugcuua aaaaguugag ucgccggcac uauaccggcu     2160

gggggaaacu cagcgcaaag cugaucaacg gaauuagaga ugaaaaaucc ggcaauacaa     2220

uccuagacua ccugauugac gaugguaucu cuaacaggaa uuucaugcag cucauacaug     2280

acgaugcccu gucguucaag aaaaagauuc aaaaggcuca gaucaucggg gacgaggaua     2340

aaggcaacau uaaggaaguu gugaaaagcc uuccaggauc ccccgccauc aagaaaggca     2400

uuuugcaguc aauaaagauc gucgacgagc uggugaaggu aaugggaggg cgcaaaccug     2460

aaaguauugu uguggagaug gcacgagaga aucaguauac uaaccaaggu aagucuaaua     2520

gccagcagcg guuaaaacgu cucgaaaagu cccugaaaga gcuuggcuca aagauccuga     2580

aggaaaacau uccagccaaa uugagcaaga ucgauaauaa cgcucuccag aaugacagac     2640

uguaccuaua uuaccugcaa aacgggaaag auauguauac cggagacgau cucgacauag     2700

acaggcuguc uaacuacgau aucgaccaca uuauccccca ggccuuucuu aaggauaauu     2760

ccauugacaa caaagucuug gugaguagcg caucuaaucg cggcaagucc gaugacuucc     2820

cgucacugga ggucgugaag aaacggaaga cauuuuggua ccaguuacuc aaaaguaagc     2880

ugaucagcca gagaaaauuc gauaaccuua cuaaggcgga gagggguggg cugcuaccug     2940

aagacaaggc uggcuuuaua caacgacagc ucguugagac ccgccagauu acaaaacaug     3000

uggcccggcu guuggaugaa aaguucaaua acaaaaagga cgagaauaac agagcaguaa     3060

ggaccgucaa aaucaucacu cugaagucga cguuaguguc ccaguuucgu aaagauuucg     3120

aacucuauaa gguucgcgag auuaaugacu uucaccaugc ccacgaugcu uaccugaacg     3180

ccgugauagc aucugcccuu cugaagaaau aucccaaguu ggagccagag uucgucuacg     3240

gagacuaucc uaaauacaau agcuuucggg aaagaaaguc agcuacagaa aaaguguauu     3300

ucuacaguaa caucaugaau auuuucaaga aguccaucuc ucucgcggac ggcaggguaa     3360

uugagcgacc ccugaucgaa gugaacgagg agaccgggga aagcguuugg aauaaagagu     3420

ccgaucuagc cacuguccgg cgcgugcugu cauacccaca agugaacguc guuaagaaag     3480

uggaagaaca gaaccacgga cuugacagag guaagcccaa aggccucuuu aaugcuaacc     3540

ugagcaguaa gccuaagcca aauucuaacg aaaauuuggu gggggcaaaa gaguaucugg     3600

auccgaagaa auacggaggc uaugccggua uauccaacag cuucgcugua uuagucaagg     3660

ggaccaucga gaaaggcgcc aagaagaaaa uuacaaaugu gcucgaauuu cagggaaucu     3720

cgauucugga caggaucaac uaccggaagg auaaacuuaa uuuccuguug gagaagggcu     3780

auaaagacau agaacucauu aucgagcugc ccaaguacuc acuauuugag cugucugaug     3840

ggagucgaag aaugcuugca uccauccuca gcaccaacaa uaaacgcgga gaaauucaua     3900

agggcaacca aaucuuccug ucucagaagu uuguuaaauu guuauaucac gcgaagagga     3960

uaagcaauac uauuaacgag aaucaucgua aauacgugga aaaccacaag aaagaguucg     4020

aggaacuguu uuacuauauc cucgaguuca augaaaacua cgucggugcc aagaagaacg     4080

ggaaacugcu uaauuccgcu uuucagucau ggcagaacca caguauugac gagcugugua     4140

gcucuuucau cggaccuaca ggcuccgaac ggaaggguuu guuugagcua acgucaagag     4200

gcagugccgc agauuucgag uuccuggggg ugaaaauacc aagguaucgc gacuacaccc     4260

ccagcucccu ccugaaggau gcuacucuua uucaucaauc uguuacagga uuauaugaaa     4320

cccgaaucga ccuggccaaa cucggcgagg ggaagagacc ugcagccaca aagaaagcug     4380

gacaggcgaa gaaaaagaaa uaaggacuag uguccaccug ucccuccugg gcugcuggau     4440

ugucucguuu uccugccaaa uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         4556


<210>  3686
<211>  3632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3686
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaug guacgccagc cugauguccg cgcaucaccu caggguugga auugacgugg      180

guacccauuc uguaggccuu gcuacacugc ggguggauga ccacgggacu ccaauagagu      240

uguuaucagc ccugagucau auccacgaua gcggagucgg caaggaaggu aaaaaggacc      300

acgauacgcg caagaaacua uccgggauug cacgacgugc cagaaggcuc cugcaucacc      360

ggcgcaccca gcuucaacag cuggacgagg uguugagaga ucucggauuc ccgaucccca      420

caccuggcga auuucuggac cugaacgagc agacugaucc auauaggguu uggcgggugc      480

gcgcucgacu cgucgaagag aaguuacccg aggaacugag aggcccugca auuucuaugg      540

ccgugaggca uaucgcucgg caccgugggu ggcgcaaucc auacucgaaa guagagucac      600

uuuugagccc cgccgaagag aguccuuuca ugaaggcgcu gagagagagg auacuagcaa      660

ccacaggaga aguucucgac gaugguauca ccccaggcca ggccauggcu caaguggccc      720

ugacucacaa cauuuccaug cgggggccgg agggaauccu uggcaaacug caucagucug      780

acaaugcaaa cgaaauucga aagaucugcg cucgccaggg ugucagcccc gaugugugua      840

agcaguugcu cagagccgug uuuaaagcgg acuccccuag ggggucagcc gucagucggg      900

uugcaccaga cccccugccu ggacaaggca gcuuccgcag agcuccaaag ugcgaucccg      960

aguuucagag guuccguaua auuucuaucg uggccaauuu acggauuucc gaaacaaaag     1020

gcgagaaccg accgcugacc gcugacgaga gaaggcacgu agugacguuu cucacugaag     1080

auucacaggc cgaccuuacc ugggucgaug uggcagagaa gcuggggguu caucgccggg     1140

acuugagagg aacagccgug cacacugaug acggugaaag gagcgcugcg cgcccuccca     1200

ucgaugcaac cgaccgaauc augcggcaga caaaaauuag uucucuaaag accugguggg     1260

aggaagccga uuccgagcaa cguggcgcua ugauaagaua ucuguacgag gacccaacgg     1320

acagcgaaug ugccgagauc auugcagaac ucccugagga agaucaggcc aaacuggacu     1380

cgcuucaucu gcccgcuggg agggccgcau auucucgcga guccuugacu gcgcucucag     1440

aucacaugcu ggcuacaacc gacgauuuac acgaggcccg gaagagacug uucggagucg     1500

acgauaguug ggcaccaccc gccgaagcua ucaaugcccc ugugggcaac ccaagcguag     1560

acaggacucu aaagauaguu ggucgauacc ucuccgcagu ggagucuaug ugggggacac     1620

cggaagucau ucauguggag cacguccgcg auggcuuuac cagcgagcgg auggcugacg     1680

aaagagauaa agccaauagg cgucgcuaua acgacaauca ggaggcgaug aagaaaaucc     1740

aacgggauua cggaaaggaa ggcuacaucu caagagggga cauugugagg cuggacgccc     1800

uugaguugca gggaugcgcu ugucuguauu gcgguacgac caucggcuac cauacauguc     1860

agcucgauca cauuguuccc caggcagggc cuggaaguaa caaucgacgg ggcaaccugg     1920

uggccguaug cgaacgcugu aauagaucca aaagcaacac uccauucgcu gucugggccc     1980

aaaagugcgg uauaccccac guggggguua aggaggcaau cggcagggug cggggauggc     2040

gaaaacagac cccuaacaca ucuucagagg accuuacuag acugaagaaa gaagucauug     2100

cgcgcuugag gcguacccag gaggauccag aaaucgacga gcggaguaug gaaagcgugg     2160

ccuggauggc uaaugagcuc caucacagaa ucgccgcagc uuaucccgag acaaccguaa     2220

ugguguacag gggcuccauu acugccgcag cccgcaaggc ugcggggaua gauucucgaa     2280

ucaaccugau uggagaaaaa gguagaaagg accggaucga uaggcgccau cacgccguug     2340

acgcaucggu cguggcuuua auggaggcca gcguggccaa gacgcuggcu gaacguuccu     2400

cacuaagagg cgagcagcgg cucacaggga aagaacaaac cuggaagcag uauacuggaa     2460

guaccgucgg cgcaagggag cauuuugaga uguggcgcgg ucacaugcug caccuuacag     2520

aauuguucaa ugagcgacug gccgaagaua aaguuuacgu gacccagaac auuagacucc     2580

ggcugucuga cgggaaugcg cauacuguga acccgagcaa guuaguaucc cacaggcugg     2640

gcgauggacu uacaguccag caaaucgacc gcgcuuguac gccugccuug uggugcgcac     2700

ucaccagaga gaaagauuuu gacgagaaga auggccugcc agccagggaa gaccgggcua     2760

uacgugugca ugggcacgag aucaagucaa gcgauuauau ucagguuuuc ucuaaacgca     2820

agaaaacuga cuccgaucga gacgaaacac ccuucggagc aaucgccgug agaggcgguu     2880

uugucgagau ugggccuagu auccaucacg cgaggauaua ccggguggaa ggaaagaaac     2940

ccguuuacgc uaugcuacgc guguucaccc acgaucugcu cagccagaga cauggcgacc     3000

uguuuucugc cguaauucca ccucagucca ucucaaugag gugcgcagag cccaaacuuc     3060

ggaaagccau uacaacuggu aacgcuaccu auuugggcug ggucguggug ggggaugagc     3120

uggaaaucaa ugucgacagu uucacgaagu acgccauagg acgauuuuua gaggauuucc     3180

caaacaccac aagauggcgc aucuguggcu augacacuaa uagcaagcuc acccugaaac     3240

cgauuguucu ugcagcugaa gggcuggaga acccuucguc cgccgugaau gaaaucguag     3300

aguugaaggg auggagggug gcgauuaacg uccucacaaa agugcacccc acuguugugc     3360

ggcgugaugc ccugggcaga ccaagguacu cuagccgcuc aaaucuaccc accaguugga     3420

cgaucgagaa gcggccugca gcuacaaaga aggccgguca agcuaagaaa aagaaauaag     3480

gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu gccaaauaaa     3540

caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3632


<210>  3687
<211>  4493
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3687
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaac caagccauac agcauuggac uggacauagg uacaaacucc guuggcuggg      180

ccgugacuac ggauaauuau aaaguaccgu cuaagaagau gaaagugcuc gggaacaccu      240

caaagaaaua caucaagaaa aaucuucugg gagucuuguu auucgacagu ggcauuacag      300

cggaggguag gcggcugaag cgcacugcuc gacguagaua uaccaggcgg cgcaacagaa      360

uccuauaccu ccaggaaauu uuuagcacag agauggccac ccuggaugac gcauucuuuc      420

aaaggcuuga ugacuccuuc cuggugcccg augacaagcg ggauucuaaa uauccuaucu      480

uugggaauuu gguugaagag aaggccuacc augacgaguu cccaacuaua uaucaccucc      540

gcaaauaccu ggcugauucg acaaagaaag cagaccugcg acucguguac uuagcccugg      600

cucauaugau caaguauaga ggacacuuuc uuauugaggg cgaguucaac ucaaagaaua      660

acgauaucca gaagaauuuu caggacuucu uggacaccua caacgccauu uuugaaagcg      720

aucugagucu agaaaauucc aagcagcucg aagagaucgu caaagacaag auaucuaaac      780

uggagaagaa agauaggauu cuuaagcugu uccccggcga aaagaacagc gggaucuuuu      840

ccgaguucuu gaaacucauu gugggaaauc aagcggacuu ccggaagugc uuuaaccugg      900

augaaaaagc aucauuacau uucaguaagg agagcuauga cgaagaucug gagacgcucc      960

uugguuacau cggcgacgau uauucugacg uauuucugaa agccaagaag uuguacgaug     1020

cuauccuacu guccggguuc cucacuguua ccgacaauga gacagaagcc ccucugucaa     1080

gcgcaaugau uaaacguuau aacgagcaca aggaagaccu ugcucuguug aaagaguaca     1140

uacgcaacau cagucucaag acuuacaaug aaguguuuaa agaugacacc aagaacggau     1200

augccggcua cauugauggu aagacaaauc aggaggacuu cuaugucuac cugaaaaagu     1260

uacuggcgga guuugaaggg gccgauuauu uccuagagaa aaucgacaga gaagauuuuc     1320

ucaggaagca gcggaccuuc gacaacggau cuauaccaua ccagauucac cuucaagaga     1380

ugcgagcaau ccuugauaaa caggcuaagu uuuaucccuu cuuggccaaa aauaaggaac     1440

gcaucgagaa gauucugacg uuuagaaucc cuuacuacgu gggcccacuc gcuaggggca     1500

acuccgacuu cgccuggagc auucggaaac gcaaugagaa gauaacuccg uggaacuucg     1560

aagaugugau cgacaaagag ucgucugcag aagccuuuau uaauagaaug acauccuucg     1620

aucuguaucu ucccgaggag aagguccugc cuaaacauuc auugcucuac gaaaccuuua     1680

acguuuauaa ugagcugacu aaggugaggu ucaucgcuga aaguaugcgu gacuaccagu     1740

uuuuagacag caagcagaaa aaggauaucg uacggcugua uuucaaagac aagcgaaaag     1800

ugacagauaa ggacauuaua gaguaccuac acgcgaucua uggguacgau ggaauugaac     1860

ucaaggguau cgagaaacaa uuuaacuccu cucugagcac cuaccaugac cuuuugaaua     1920

uuaucaacga uaaggaguuc cuggacgauu caaguaauga agcaauaauc gaggaaauua     1980

uccacacgcu caccauuuuu gaggacagag aaaugaucaa acagaggcug uccaaguucg     2040

agaacauauu ugauaaaagc gucuuaaaga agcugucucg ccggcacuau acaggcuggg     2100

ggaaacuuuc agccaaguug auuaacggaa ucagagacga gaaaaguggc aauacuauuc     2160

ucgauuaccu gaucgacgac gguauaagca acaggaauuu caugcagcua auccaugaug     2220

acgcucuguc cuuuaagaaa aagauucaaa aagcccagau cauuggggau gaagacaagg     2280

gcaacaucaa ggaggugguu aaaucucucc caggaucgcc cgcaauaaag aaaggcauuc     2340

ugcagagcau caagaucgug gaugaacuug ucaaagugau gggagggcgc aagccugagu     2400

ccauuguagu ugaaauggcc cgagagaauc aguauaccaa ccaagguaag ucaaauaguc     2460

agcagcgguu gaaacgucug gagaagucuu uaaaagaacu cggcagcaag auccugaaag     2520

agaacauucc agcuaagcug uccaagauag acaauaacgc ccugcagaau gauagauugu     2580

accucuauua ccugcaaaac gggaaagaca uguauacagg agaugaccua gauaucgaca     2640

ggcugucaaa uuacgacauu gaucacauca uuccccaggc auuccucaag gacaacagca     2700

ucgauaauaa agugcugguc ucuuccgcga guaaccgcgg caagagcgac gaugugccgu     2760

cucuugaagu cgugaaaaag cggaagacuu ucugguacca guugcugaaa uccaaguuaa     2820

uaucacagag aaaauuugac aaccucacca aggcugagag gggugggcug aguccugagg     2880

auaaagccgg cuucauccaa cgacagcuug uugaaacacg ccagauuacc aagcaugugg     2940

cacggcugcu agacgagaag uuuaauaaca aaaaggauga aaauaacaga gccguaagga     3000

cugucaaaau cauuacgcuc aagagcacac uggugucgca guuccguaaa gacuuugagu     3060

uguauaaggu ucgcgaaauc aaugauuucc accaugcuca cgacgccuac cugaacgcag     3120

uggucgcuuc cgccuuacuc aaaaaguauc ccaagcugga gccagaguuu guguacggag     3180

auuauccuaa auacaauucu uuccgggaaa gaaagagcgc gaccgagaaa guauauuuuu     3240

acucaaacau aaugaauauu uucaagaaaa guaucucccu ugccgacggc agggugaucg     3300

aacgaccccu gauugagguu aacgaagaga cuggggaguc ugucuggaau aaggaaagcg     3360

acuuggcuac cgugcggcgc gugcucuccu acccacaagu caacguugug aagaaagugg     3420

aggaacagaa ucacggacug gauagaggua agcccaaagg ccuauuuaac gcaaaucugu     3480

caagcaagcc uaaaccaaac aguaacgaga aucuuguagg ggccaaggaa uaucucgacc     3540

cgaagaaaua cggaggcuau gcugguauau cuaacuccuu cacaguccug gugaagggga     3600

ccaucgagaa aggcgccaag aaaaagauua cuaauguuuu ggaguuccag ggaaucagca     3660

uucuggauag gaucaacuac cggaaggaca aauuaaauuu ucuccuggaa aagggcuaua     3720

aagauauaga gcuuaucauu gaacugccca aguacucguu guucgagcuc ucagacgggu     3780

cucgaagaau gcuggcaagu auccuaucca caaacaauaa acgcggagag auucauaagg     3840

gcaaccaaau cuuucugagc cagaaauucg ugaaacuucu cuaucacgcg aagaggauau     3900

cuaauacgau uaacgaaaau caucguaaau acgucgagaa ccacaagaaa gaauuugagg     3960

aacuguucua cuauaucuug gaguuuaaug agaacuacgu gggugccaag aaaaauggga     4020

aguuacugaa cagcgcuuuc caguccuggc agaaucacuc aaucgaugaa cucuguagua     4080

gcuuuauugg accuaccggc ucugagcgga agggucuguu cgaacuuacu uccagaggcu     4140

cagccgcaga cuuugaguuc cuggggguua aaaucccaag guaucgcgau uacacaccca     4200

guagcuugcu aaaggacgcu acccugauac aucaauccgu gacaggacuc uaugaaacuc     4260

gaauugaucu ggccaaacuu ggcgagggga agagaccugc agccaccaaa aaggcuggac     4320

aggcgaagaa aaagaaauaa ggacuagugu ccaccugucc cuccugggcu gcuggauugu     4380

cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4493


<210>  3688
<211>  3575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3688
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc cgcguucaag ccaaacagca uuaauuacau acugggacuc gacaucggua      180

uugcuuccgu uggcugggcc augguggaga ucgaugaaga ggaaaacccg auuaggcuua      240

ucgaccuggg gguacgggug uuugagcgcg cagaaguccc caaaaccgga gauucuuugg      300

ccauggcucg acguuuagca agaucaguga ggcggcugac acgcagaagg gcccaucggc      360

uacuccgcac ucgaagacug cuuaagaggg agggcguucu gcaggcugcc aauuucgacg      420

agaacgguuu gauaaagagu cucccuaaua cgccauggca acugcgggcg gcagcccugg      480

aucguaaacu cacccccuua gaauggagcg cugugcugcu ucacuugauc aagcaucgcg      540

gguaucuguc ccagaggaaa aacgagggag aaacagccga caaggagcua ggcgcacucc      600

ugaaaggcgu cgcugggaau gcccacgcgc uucagacugg agauuuuagg accccugccg      660

agcuggcauu gaacaaguuc gaaaaggagu cuggucauau ucggaaucag cgaucggacu      720

acucacacac auuuagccgc aaagaucucc aagcugaacu gaucuuacug uucgagaagc      780

agaaagaauu uggcaaccca cacgugagug ggggacucaa ggagggcauu gagacccuuc      840

ugaugacuca gagacccgcc uuguccggug acgcuguaca gaaaaugcua gggcauugca      900

cauucgaacc ugccgagcca aaggcagcca aaaauaccua uacggcugaa agguuuaucu      960

ggcugacuaa gcucaacaau cugcggauac uugagcaagg aucugaacgc ccgcugaccg     1020

auacagagag agcgacuuug auggacgagc ccuacagaaa gagcaaacuc accuaugcac     1080

aggcccguaa gcuguuaggc cuggaagaua cagcuuucuu uaaaggccua cgguacggga     1140

aggacaacgc cgaggcaucc acccucaugg aaaugaaagc cuaucacgcu auuucacgag     1200

cccuggagaa ggaaggacuu aaggacaaaa agaguccuuu gaaucugagc ccagagcucc     1260

aggaugagau cgguacggca uucucucugu uuaaaacuga cgaagauauu acaggcagac     1320

uuaaggacag gauccagccc gagauccugg aagcguugcu caaacauauu uccuucgaua     1380

aguucguuca aauaucacug aaggcuuuac gccggaucgu gccucugaug gagcagggga     1440

aaagauacga cgaggccugu gcagaaauuu acggagauca cuauggcaag aaaaacaccg     1500

aggaaaagau cuaccuacca cccauaccgg ccgacgagau uaggaacccu gucgugcucc     1560

gcgcucugag ccaggcccga aaagugauca auggugucgu ucggcguuau gggagucccg     1620

caagaaucca cauugaaacu gcuagggagg ugggcaaguc uuuuaaggau cgcaaagaga     1680

ucgaaaagcg gcaagaggaa aacagaaaag acagggagaa ggccgcggcc aaauuccgag     1740

aauacuuucc aaauuucgua ggagagccua aguccaaaga uauucuuaag uugcgccugu     1800

augagcagca gcauggcaag ugccucuaca gcgggaaaga aauaaaccug ggacgguuaa     1860

augagaaggg uuauguggaa aucgaccacg cucugcccuu uucgagaaca ugggacgauu     1920

cuuucaacaa uaaaguccuu guguugggcu ccgagaacca gaauaagggg aaccaaaccc     1980

cauacgaaua cuuuaaugga aaagacaacu caagggagug gcaggaguuc aaggcacgug     2040

uugaaacgag ucgcuuuccc cggagcaaga aacagaggau ucuccugcag aaguucgaug     2100

aggacggcuu uaaagaaaga aaucuaaacg auacccgaua ugugaaucgg uuccuguguc     2160

aauuugucgc cgaccgcaug agacucacag guaaggggaa aaagagggug uucgcuucca     2220

acggccagau cacuaaccug cuucggggau ucuggggcuu gcgaaaggua agagccgaga     2280

augaucgcca ucacgcacug gacgcgguug uggucgccug cucuaccgug gcuaugcagc     2340

agaaaaucac aagguuuguc cguuacaagg agaugaacgc cuucgauggg aaaacuauug     2400

acaaggaaac cggagaggug uuacaucaga aaacacacuu uccucagcca ugggaguucu     2460

uugcacagga aguuaugaua cggguguucg guaagccgga uggcaagccc gaauuugagg     2520

aggcugacac ccucgaaaaa cugagaacuc uucuggccga gaaguugagc ucaaggccug     2580

aagcaguaca cgaguauguc acgccacucu ucgugagucg cgcccccaau cgaaaaaugu     2640

ccgggcaggg acauauggaa acaguuaaga gcgcuaaaag acuggaugag ggcgugucug     2700

uccuacgggu gccucugacc caacucaagc ugaaagaccu ugagaagaug guaaacaggg     2760

aacgcgagcc aaaguuguac gaagcgcuga aagcccguuu agaggcacac aaggacgauc     2820

ccgcuaaagc cuuugccgaa ccguucuaua aguacgacaa agcugguaau agaacucaac     2880

aggugaaggc aguucggguc gagcaagugc agaagaccgg gguguggguc aggaaccaua     2940

auggcaucgc cgauaacgcg acaaugguuc gcguggacgu guuugagaaa ggagauaagu     3000

auuaccucgu accuauuuac ucauggcagg ucgcuaaagg cauccugcca gaccgagccg     3060

ugguucaggg gaaggaugaa gaggacuggc aacuuauuga ugacaguuuc aauuuuaaau     3120

ucagccugca ccccaacgau cuaguggaag ucaucaccaa gaaggcaaga auguucggau     3180

auuuugccuc cugucaccgg ggcacuggua auauaaacau caggauucau gaccucgauc     3240

acaaaaucgg gaagaauggc auucuggagg gaaucggugu gaaaacagcu uugucuuucc     3300

agaaguacca gauagacgag cugggcaaag aaauucgccc uugcagauua aagaagaggc     3360

ccccaguucg gaaacguccu gcagccacga agaaagcggg gcaggcuaag aaaaagaagu     3420

aaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu ccugccaaau     3480

aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                3575


<210>  3689
<211>  4331
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3689
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaaaccauac accauuggac uggacauagg uacaaacagc guuggcuggg      180

ccgugcucac ugaucaguau gaccuuguaa agaggaagau gaaaaucgcg ggggauuccg      240

agaagaaaca aauuaagaaa aauuucuggg gagugcggcu guuugacgaa ggccagacgg      300

cugccgaucg ccgaauggca cguaccgcca gaaggcggau cgagcgcaga aggaaccgga      360

uuucuuacuu gcaggguauc uucgcugaag agaugucaaa gacagacgca aacuuuuucu      420

gccgcuuaag ugauagcuuu uaugucgaca augaaaagcg aaauuccaga cauccguucu      480

uugccacuau agaggaggaa guggaguacc acaaaaacua ucccaccauc uaccaucuga      540

gggaggagcu aguuaauucu ucggaaaagg cugaucuccg gcugguguau cuugcccugg      600

cgcacauuau caaauaccgu gggaacuucu ugauugaagg agcacucgac acacagaaua      660

ccucagucga uggcaucuac aagcaauuua uacagacuua uaaccaggug uucgccagcg      720

gcauugagga cgggagucug aaaaagcugg aagauaauaa agacguagcu aagauccucg      780

uugagaaggu gacacgcaaa gaaaaguuag agaggauucu gaaacuuuac ccuggagaga      840

aguccgccgg uauguuugca caguucaucu cuuugaucgu cggcagcaaa gggaacuuuc      900

aaaagccauu cgaccugauu gaaaaguccg auauagagug ugcuaaagac ucauaugaag      960

aggaucuaga aagucuccug gcccuuaucg gagacgagua cgcggagcug uucguggccg     1020

caaagaaugc uuauagcgcc guggucuugu cuuccauuau caccguugcu gaaacggaga     1080

cuaacgccaa acucucagca agcaugauag aaagguuuga uacccaugag gaagaccugg     1140

gcgaguuaaa ggccuucauu aaacugcacc uccccaagca cuacgaggaa aucuuuagua     1200

auacagagaa gcaugguuau gcuggguaca ucgauggaaa aacuaagcag gcggacuucu     1260

acaaauauau gaagaugacc cuugaaaaca uugagggcgc agauuacuuu aucgccaaaa     1320

uugagaagga aaacuuccug cggaagcagc gaacauuuga caauggcgcu auaccucacc     1380

aguugcaucu agaggaacug gaggccaucc uccaccaaca ggcaaaauau uacccauucc     1440

ugaaggaaaa cuaugauaaa auuaagucuc uugugaccuu ucgcaucccc uacuucguag     1500

ggccucuggc caauggacag uccgaguuug cuugguugac gagaaaagcc gacggugaga     1560

ucaggccaug gaacauugaa gagaaggugg acuucggcaa aagcgcaguc gauuuuauag     1620

aaaagaugac uaauaaggac acauaucucc cgaaagagaa cgugcugccc aagcacucgu     1680

uaugcuacca aaaauaccug guuuauaaug aacuaaccaa ggugcgguac aucaacgauc     1740

aggggaaaac uucuuauuuc uccggacagg agaaggagca gauuuucaau gaccucuuua     1800

agcaaaaacg caaggucaaa aagaaagauc uggaacuuuu cuugagaaac augucacaug     1860

uggagagucc uacaaucgaa ggccuggagg acagcuuuaa uuccucuuac agcaccuauc     1920

acgaucuccu gaagguaggu auuaagcagg aaauccuuga caacccaguu aauacggaga     1980

ugcuggagaa cauagugaaa aucuugaccg ucuucgaaga uaagaggaug auuaaagagc     2040

agcuccagca auuuucagac gugcuggaug gggucguguu aaagaaacug gaacgucggc     2100

auuacacagg cuggggacga cuaagugcga agcuccugau gggcaucaga gacaagcagu     2160

cccaccuuac uauuuuggau uaucugauga augacgacgg gcucaacagg aaccugaugc     2220

aguuaaucaa ugauagcaac cugucuuuca aaucaauaau ugagaaggag cagguuacca     2280

cagcugacaa agauauccaa aguauugugg ccgaccuugc aggaagcccc gccaucaaga     2340

aagguauauu gcagucccuc aagaucguag augaacuggu cucugugaug ggcuacccuc     2400

cacagacuau uguuguggag auggcucgcg aaaaucagac cacagggaaa ggaaagaaca     2460

auucgcggcc cagauacaag agccuagaga aagccaucaa ggaauuuggc ucccaaauuc     2520

ugaaagagca uccgaccgac aaccaggagc ucaggaauaa ccgccuguau cuuuacuauu     2580

ugcagaaugg uaaggauaug uacacugggc aagaccugga uauccacaac uuaucaaauu     2640

augacauaga ucacauuguc ccucagaguu ucaucacgga caacucuauc gauaaucucg     2700

ugcugacaag cuccgcaggc aaccgagaaa aaggagacga cguaccccca cuugagauug     2760

ugcggaagcg uaaggucuuu ugggaaaaac uguaccaggg caauuugaug ucaaagagaa     2820

aauucgauua ucucaccaag gcugagagag ggggacugac ugaagccgac aaagcgcgcu     2880

uuauccaucg gcagcuaguu gagaccagac aaauuacaaa gaacguggcc aacauacugc     2940

accagagguu caauuacgag aaggaugacc augguaacac caugaaacag gugcgaaucg     3000

ucacucucaa gagcgcucug guuucucagu uucgcaaaca auuccagcuu uacaaggugc     3060

gggaugugaa ugacuaucac cacgcacaug augccuacuu gaacggcgua gucgcuaaua     3120

cacuguuaaa aguguauccu cagcucgaac ccgaguucgu uuacggggac uaucaccagu     3180

uugauugguu caaggccaac aaggcaacgg cgaaaaagca auuuuacacc aauauuaugc     3240

uguucuuugc ccagaaagac agaaucauug augaaaacgg agagauccuu ugggacaaga     3300

aauaucugga cacugugaag aaggucaugu ccuacaggca gaugaauaua gugaaaaaga     3360

cagaaaucca aaaaggcgag uucaguaagg cuaccauuaa accaaagggu aacagcucua     3420

aacuaauccc ccguaagaca aauugggauc cuaugaagua cgggggccuc gacuccccaa     3480

acauggccua ugcaguugug auugaguacg cuaaaggaaa gaauaaacug guauuugaaa     3540

agaaaaucau acgcgucacu auuauggagc ggaaggccuu cgaaaaggau gagaaagcau     3600

uuuuggagga acagggcuau agacagccga aggugcuggc caaauuaccc aaguacaccc     3660

ucuaugagug ugaagagggg aggcgacgga ugcuggcuuc agcgaacgaa gcccagaaag     3720

gaaaucaaca ggugcuuccu aaccaucugg ucacguugcu ccaccaugca gcuaacugcg     3780

agguuaguga cggcaagagc cuggauuaca ucgagucgaa ucgcgaaaug uucgccgagc     3840

uacuggccca cguguccgaa uuugcuaaga gauauacccu ugcagaggcc aaccucaaua     3900

aaaucaacca gcuguucgaa cagaauaagg agggugacau uaaagcgaua gcucaaucuu     3960

ucguagauuu gauggccuuu aacgcaaugg gggccccagc uagcuucaag uuuuucgaga     4020

caacuaucga aaggaaacgg uacaauaacc ugaaggaguu acucaauuca accauuaucu     4080

accagaguau uacaggacug uaugaauccc gaaagagacu ugacgauaaa cgccccgcag     4140

ccacuaagaa agcgggccag gcuaagaaaa agaaauaagg acuagugucc accugucccu     4200

ccugggcugc uggauugucu cguuuuccug ccaaauaaac aggaucagcg cuuuacagau     4260

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaagaau u                                                          4331


<210>  3690
<211>  4364
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3690
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa gaaaccauac agcauuggac uggacauagg uaccaacucc guuggcuggg      180

ccgugguaac agaugacuau aaggugccgg cgaagaaaau gaagguccuc gggaauacug      240

auaaaucuca uaucgagaag aaccuucugg gagcuuuguu auucgacuca ggcaauacgg      300

ccgaagauag gcggcugaaa cgcaccgcac gacguagaua cacaaggcgg cgcaaccgga      360

uucuauaucu ccaggagauc uuuagugaag agauggguaa gguggacgau agcuucuuuc      420

acaggcugga agacuccuuc cuuguuacug aggauaagag aggggagcgc caucccauuu      480

uuggaaaucu ggaagaggaa gugaaauacc acgagaacuu cccuaccauc uaucauuugc      540

gacaauaccu cgccgacaau ccagagaagg ucgaucugag acugguguau cucgcuuuag      600

cacacauaau caaauuuagg ggccacuucc ugauugaagg caaguuugac acacggaaca      660

augauguaca gcgucuuuuc caggaguuuu uggccguuua cgacaacacc uucgaaaauu      720

cuucgcugca ggagcaaaac gugcaggucg aagagauccu aacugacaaa auuucaaaga      780

gcgcuaaaaa ggaucgcgug cucaagcugu uucccaauga gaaaaguaac gggagauucg      840

ccgaguuccu uaagcugauc gugggaaauc aggcggacuu uaaaaagcau uucgaguugg      900

aagagaaagc accucuccag uuuuccaagg auacauacga agaggagcug gaagucuuac      960

uggcccaaau aggugacaac uaugcugaac ucuuccuuuc ugccaagaaa cuguacgaua     1020

gcauuuugcu auccggcauc cugaccguua cggacguggg gacuaaggca ccacucucag     1080

cuaguaugau ucagagguau aacgaacacc agauggaucu ggcccagcuu aaacaauuua     1140

uccggcagaa gcugagcgac aaauacaaug agguauucuc ugaugugucc aaggacggau     1200

augcgggcua caucgauggu aagaccaacc aggaagccuu uuauaaauac uugaaggggc     1260

uccugaauaa aauugaggga ucaggcuacu ucuuagacaa gauagagcga gaagacuuuc     1320

ugcgcaaaca aagaacauuc gauaacggca gcauccccca ucagauucac cuacaggaga     1380

ugagggcaau cauacggcgc caggcugaau uuuauccuuu ccucgccgac aaucaagaua     1440

gaauugagaa gcugcuuacu uuuaggaucc cauacuaugu cgggccguug gcucguggaa     1500

agagugacuu cgccuggcug ucucggaaau ccgcagauaa gaucaccccc uggaacuucg     1560

acgagauugu ggauaaagaa agcucggccg aggcuuuuau caaucgaaug acaaacuacg     1620

accucuaucu gccuaaucag aagguucuuc caaaacacuc ucuguuguac gaaaaguuca     1680

ccguguauaa cgagcucacg aaagucaagu acaagacuga acaggguaaa acagcguuuu     1740

ucgaugcaaa uaugaagcag gagauuuuug acggcguguu caaaguauac agaaagguua     1800

ccaaagauaa gcugauggac uuuuuagaga aggaguucga ugaauuuagg auaguggacc     1860

ugacugggcu agacaaagaa aacaaggucu ucaaugccuc cuauggaaca uaccaugauc     1920

ucugcaaaau ccuggacaag gauuuucuug acaacaguaa aaaugagaag auuuuggaag     1980

auaucgugcu gacccucacg cuguucgagg accgcgagau gauccggaag agauuagaaa     2040

acuauucaga ucugcuuacc aaagagcaag ucaagaaauu ggaaaggcgc cacuacacag     2100

gcuggggucg acucagcgcu gagcugauuc augggauacg gaacaaggaa ucccguaaaa     2160

cuauccuaga cuaucugauu gaugacggca auucuaacag aaauuuuaug cagcucauca     2220

acgaugacgc ccugagcuuc aaggaggaga uugcaaaggc ccaggugauc ggagaaaccg     2280

auaaucuuaa ccagguugug ucagacauag cuggcagucc cgccaucaaa aaggggauuu     2340

ugcaaucccu gaaaaucgua gacgaguuag ucaagauuau gggacaccag ccugaaaaua     2400

ucgugguuga gauggcaagg gagaaccagu ucacaaauca gggucgccgg aacagccaac     2460

agagacucaa aggccugacu gauucuauaa aggaauuugg gucacagauu cuuaaagagc     2520

auccagugga aaauagucaa cugcagaacg acagguuguu ccucuacuau cugcagaaug     2580

gacgagauau guacaccggc gaggaacuag acaucgauua ccugagccag uaugacauug     2640

aucacaucau accccaagcg uuuaucaagg acaacuccau ugauaaucgc guccucacau     2700

cuucgaagga gaaccggggu aaaagcgacg augugccguc caaggacgua gugagaaaaa     2760

ugaagucaua cuggaguaaa cugcuuucug cuaaguugau cacccagagg aaguucgaca     2820

accugacuaa agccgagcgu gggggcuuaa cggaugacga uaaggcagga uuuauuaaac     2880

gccagcucgu ugaaacacgg cagaucacca agcacgucgc cagaauacug gacgagaggu     2940

ucaauacuga aaccgaugag aacaauaaaa agauucgaca agugaagauc gugacacuua     3000

aaagcaaccu ggucuccaau uuucggaagg aguucgaacu auauaaaguu cgcgagauca     3060

acgacuacca ucacgcucau gaugccuauc ucaaugcagu gauuggcaag gcucuguugg     3120

ggguguaccc ucagcuggaa cccgaguuug uauauggaga cuacccacac uuccacgguc     3180

auaaagaaaa caaggccacc gcgaagaaau uuuucuacuc aaauaucaug aacuucuuua     3240

agaaagauga cgucagaacu gauaagaaug gcgagauuau auggaaaaag gacgaacaca     3300

ucagcaacau uaagaaagug uuaucuuauc cucagguuaa uaucgugaag aaagucgagg     3360

agcagacagg gggauucucc aaggaaagua uucuccccaa aggcaacagc gauaagcuga     3420

ucccaaggaa aacgaagaag uuuuacuggg acaccaaaaa guaugguggg uucgacucuc     3480

ccauaguggc cuacuccauc cuuguuauug cugauaucga gaaaggcaag aguaaaaagc     3540

ugaagacugu gaaagcauug guaggaguca caauuaugga aaagaugacc uuugagcggg     3600

acccuguggc cuuccucgag cgaaaaggcu auagaaaugu gcaagaagag aacaucauaa     3660

agcugccaaa auacucacua uuuaagcugg aaaacgggcg caagaggcuu cucgcuagcg     3720

cccgugagcu gcagaaagga aaugaaauug ucuugccgaa ccaucugggc acauuacucu     3780

aucacgcaaa gaauauccau aaaguugaug agcccaagca ccuggacuac guggauaaac     3840

acaaggacga guucaaggaa cuucuggaug uagugucgaa cuuuuccaaa aaguacacuu     3900

uggcggaggg uaaucucgag aaaaucaagg aacuguaugc ccagaacaau ggggaagacc     3960

uaaaagagcu ggcuucuagc uucauuaacc uucucaccuu uacggccaua ggagcuccug     4020

caaccuucaa guuuuucgau aaaaauaucg accggaagag auacacauca acuaccgaga     4080

uucugaacgc cacauugauc caucaaagua uuacuggcuu auaugaaacc aggaucgauc     4140

ugaauaagcu cgguggcgac aaacgcccag cagccacgaa gaaagcuggg caggcgaaga     4200

aaaagaagua aggacuagug uccaccuguc ccuccugggc ugcuggauug ucucguuuuc     4260

cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4364


<210>  3691
<211>  3497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3691
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaca gacaacaaac cugagcuaca uucucggacu ugaccugggu auagccuccg      180

uuggcugggc ggugguagag aucaaugaaa acgaggaucc aauuggguug aucgacgugg      240

gagucaggau uuucgaacgg gcugaggugc cgaagacugg cgaaucuuua gcccugucac      300

gccgacuagc acguaguacg agaaggcuca uccggcgcag agcccauagg cugcuucugg      360

cuaaacgguu uuugaagcgc gaggguauac ucagcaccau cgaucuggag aaggggcugc      420

ccaaucaagc augggaacuc cgaguugccg gauuagagag aaggcugucc gcuauugaau      480

ggggcgccgu gcuuuugcac cugaucaaac aucggggcua ucuaucuaag cguaaaaacg      540

agucgcagac aaauaacaag gagcucgggg cgcugcuuuc aggagucgca cagaaucacc      600

agcuguugca aagcgacgau uaccgcacuc cugccgaacu cgcucugaaa aaguucgcca      660

aggaggaagg ucauauuaga aaccagaggg gcgcauauac ccacacauuu aaucgguuag      720

accugcucgc ugagcuuaac cuguuguucg cccagcagca ccaauuuggg aauccacauu      780

gcaaagaaca cauccagcag uacaugaccg agcuacugau guggcagaag cccgcgcuca      840

guggagaggc cauacugaaa augcuuggca aguguacuca ugagaaaaac gaguucaagg      900

cagcuaaaca cacauauucc gccgaacgau uuguguggcu gaccaaguug aauaaccucc      960

gcauucugga ggauggugcu gaaagagccu uaaaugagga ggaaaggcaa cugcuaauca     1020

accacccuua cgagaagucu aaacucacgu augcacaggu acggaagcug cuuggguuga     1080

gcgaacaggc cauuuucaaa caucugcgcu acuccaagga gaacgcugaa ucagcgacuu     1140

uuauggagcu caaagcaugg cacgccauca gaaaggcucu ggagaaucaa ggacuuaagg     1200

acaccuggca ggaucuggcc aaaaagccag acuugcucga ugaaaucggc acagcauuca     1260

gucuguacaa aacugacgag gauauucagc aguauuuaac caacaagguu cccaauagcg     1320

ugauaaacgc ccugcuaguc ucucucaauu uugacaaauu caucgaacug ucccuuaagu     1380

cauugaggaa gauucugccu cucauggagc aaggcaaacg uuacgaccag gcuugccggg     1440

agaucuaugg gcaucacuac ggagaagcca accagaagac aagccagcug uuaccagcaa     1500

uaccggcgca agagauucga aaucccgugg ugcugagaac ccuuagucag gcuaggaaag     1560

ucaucaacgc caucauucgc caguaugguu cuccugcacg gguucauauc gaaacgggca     1620

gagaguuggg gaaguccuuu aaagaaaggc gcgagauuca gaagcaacag gaggauaauc     1680

gaacuaagcg ggaaagcgcc gugcagaaau ucaaggaacu cuucucggac uuuucuuccg     1740

aaccaaaauc aaaggauaua cugaaauucc gucuauacga gcagcaacac ggaaaguguc     1800

uguauagugg caaagaaauc aacauucaca gacucaauga gaaggguuac guagagaucg     1860

accaugcucu gcccuuuagc aggacauggg augacuccuu caacaauaag gugcuugucu     1920

uggccucuga aaaccagaau aaagggaacc agaccccuua cgaguggcug caaggcaaga     1980

ucaacagcga acgcuggaaa aauuuugugg cauuaguucu cggaucacag ugcagugcug     2040

ccaagaaaca gcggcugcuu acucagguga uugaugacaa caaguucaua gauagaaauc     2100

ugaacgacac aagguauauc gcgcgauuuu uguccaauua cauucaagag aaccuccugc     2160

uagucggcaa gaauaaaaag aacguguuca ccccaaaugg gcagaucacg gcccugcucc     2220

gcagccggug gggacugauu aaagcuagag aaaacaauaa caggcaccau gcacuugaug     2280

ccaucguagu ugcuugugcc acccccucua ugcagcagaa gauaacacgu uuuauccgcu     2340

ucaaagaggu gcacccguau aagauugaga aucgguacga aauggucgac caagagucag     2400

gugaaaucau uaguccucac uuucccgagc caugggcaua uuucagacag gaggugaaca     2460

ucagggucuu ugacaaucau ccugauacug uguugaagga aaugcugccc gaccgaccac     2520

aggcgaacca ccaguucguu caacccuuau ucgugagccg ggccccuacc cgcaaaaugu     2580

ccggccaggg gcauauggag acaauaaagu cugcuaaaag acucgccgaa ggaauuucgg     2640

uacugaggau cccacuuacu cagcugaagc cgaaccuacu cgagaauaug gucaacaaag     2700

aacgggagcc cgcacuguac gcuggcuuga aggcccgacu ggcagaguuu aaucaagauc     2760

cugccaaggc uuucgcgacc ccauuuuaua aacagggugg gcaacaggug aaggccauua     2820

gaguugaaca ggugcagaaa agcggcgucu uagugcgcga gaacaaugga guagcagaca     2880

acgcuuccau cgugaggaca gauguuuuca uaaagaauaa caaauuuuuc cucgucccca     2940

ucuacaccug gcaaguggcc aagggcauuc ugccuaauaa agccaucgug gcucacaaga     3000

acgaagacga gugggaagag auggaugagg gggcaaaguu uaaauucuca cuuuuuccaa     3060

augaccuggu cgaauugaag acuaaaaagg aguacuucuu uggauauuac auuggucucg     3120

aucgugccac gggcaacauc agucugaaag aacaugacgg ggagauaucu aagggaaagg     3180

auggcguuua ucgggugggu gugaaacuag cgcugagcuu cgaaaaguac cagguagacg     3240

agcuugggaa aaauagacag auuugcaggc cccagcagcg ccagccgguc cgaaagagac     3300

cugcugccac caaaaaggca ggccaagcca agaaaaagaa auaaggacua guguccaccu     3360

gucccuccug ggcugcugga uugucucguu uuccugccaa auaaacagga ucagcgcuuu     3420

acagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aagaauu                                                    3497


<210>  3692
<211>  3575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3692
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc cgcguucaag ccaaacccga uuaauuacau acugggacuc gacaucggua      180

uugcuagcgu uggcugggcc augguggaga ucgaugaaga cgagaacccc auuugccuua      240

ucgaucuggg gguaagggug uuugaacggg cagagguccc uaaaaccgga gacuccuugg      300

ccauggcucg ccgauuagca cguucuguga gaaggcugac acggcgcaga gcccauaggc      360

uacuccgggc ucgccgacug cuuaagagag aaggcguucu gcaggccgcg gauuucgacg      420

agaaugguuu gauaaaguca cucccaaaca cucccuggca acugagggca gccgcucugg      480

aucggaaacu cacgccuuua gaguggagug ccgugcugcu ucacuugauc aagcaucgug      540

gguaucugag ccagcgcaaa aaugaaggag agaccgcaga caaggaacua ggcgcucucc      600

ugaaaggcgu cgccgauaac gcgcacgccc uucagacagg ggacuuuaga acuccagcag      660

agcuggcuuu gaauaaguuc gagaaggaau ccggacauau uaggaaccag cggggugauu      720

acucucacac cuuuucgcga aaagaccucc aagccgagcu gaucuuacug uucgaaaagc      780

agaaagaguu uggcaauccc cacgugucag ggggacucaa ggaaggcauu gagacacuuc      840

ugaugaccca gcgcccugcu uugagcggug augccguaca gaaaaugcua gggcauugua      900

cuuucgagcc agcagaaccg aaggccgcua aaaacacaua uaccgcggag agauuuaucu      960

ggcugacgaa gcucaauaac cugaggauac uugaacaagg aagugagcgg ccccugacug     1020

acaccgaacg cgcaacauug auggacgagc cuuacagaaa guccaaacuc acuuaugccc     1080

aggcuaggaa gcuguuaggc cuggaggaua ccgccuucuu uaaaggccua cguuacggga     1140

aggacaaugc agaagccucu acacucaugg agaugaaggc uuaucacgcc auuagccggg     1200

cacuggaaaa ggagggacuu aaggauaaaa aguccccauu gaaccuguca cccgaacucc     1260

aggacgagau cgguaccgcg uucagucugu uuaaaacgga ugaggacauu acuggccgac     1320

uuaaggauag aauccagccu gaaauccugg aggcuuugcu caaacauauu agcuucgaca     1380

aguucguuca aauaucucug aaggccuuaa ggcgcaucgu gccacugaug gaacagggga     1440

aacgguacga ugaggcaugc gccgagauuu acggagacca cuauggcaag aaaaauacag     1500

aagagaagau cuaccuaccc ccgauaccug cugaugaaau uagaaacccc gucgugcuca     1560

gggcccuguc ccaggcacgc aaagugauca acggugucgu ucgacgguau gggucaccag     1620

cucguaucca cauugagacc gccagagaag ugggcaagag cuuuaaggac aggaaagaga     1680

ucgagaagcg ccaagaagag aaucggaaag acagagaaaa ggcggccgcu aaauucaggg     1740

aguacuuucc uaacuucgua ggagaaccca agaguaaaga uauucuuaag uugcgacugu     1800

augagcagca gcauggcaag ugucucuacu cugggaaaga gauaaaucug ggacgcuuaa     1860

acgaaaaggg uuauguggag aucgaccacg cacugccauu uucccggacu ugggaugaca     1920

gcuucaauaa caaaguccuu guguugggcu cggaaaauca gaacaagggg aaucaaacac     1980

ccuacgagua cuuuaacgga aaagauaauu cuagagaaug gcaggaguuc aaggccaggg     2040

uugagaccuc ccguuuuccu cgcucaaaga aacagcggau ucuccugcag aaguucgacg     2100

aagauggcuu uaaagagaga aaccuaaaug acacgaggua ugugaaccga uuccugugcc     2160

aauuugucgc ugaucggaug cgccucaccg guaaggggaa aaagagagug uucgccagua     2220

auggccagau cacaaaccug cuuaggggau ucuggggcuu gcggaaggua cgagcagaaa     2280

acgacagaca ucacgcgcug gaugccguug uggucgcuug uagcacugug gccaugcagc     2340

agaaaaucac ccgcuuuguc agguacaagg agaugaaugc auucgacggg aaaacaauug     2400

auaaggagac uggagaagug uuacaucaga aaacccacuu uccacagccg ugggaguucu     2460

uugcucagga aguuaugaua cguguguucg guaagcccga cggcaagccu gaguuugaag     2520

aggccgacac accagagaaa cuccggaccc ugcuugcaga aaagcugucc ucuagacccg     2580

aggccguaca cgaauauguc acuccuuugu ucgugagcag ggcuccaaac cgcaaaaugu     2640

cagggcaggg acauauggag acgguuaaga gugcgaaacg acucgaugaa ggcguguccg     2700

uccugagagu gccccuaaca caacugaagc ucaaagaccu ggagaagaug guaaaucggg     2760

agagggaacc gaaacuuuac gaggccuuga aagcacgccu ggaagcucac aaggaugacc     2820

cugccaaagc cuuugcugag ccauucuaua aguacgauaa agcagguaac cguacccaac     2880

aggugaaggc cguuagaguc gaacaagugc agaagacugg gguguggguc cggaaucaua     2940

acggcaucgc ggacaaugcu accaugguua ggguggaugu guuugagaaa ggagacaagu     3000

auuacuuagu acccauuuac agcuggcagg ucgccaaagg cauccucccu gaucgcgcag     3060

ugguucaggg gaaggacgag gaagauuggc aacugauuga cgauucuuuc aacuuuaaau     3120

ucucacuuca ccccaaugac cugguggagg ucaucacaaa gaaggcccga auguucggau     3180

auuuugcuag uugccacaga ggcaccggua acauaaauau ccggauucau gaccuagauc     3240

acaaaaucgg gaagaacggc auucucgaag gaaucggugu gaaaacugca cugagcuucc     3300

agaaguacca gauagacgag uugggcaaag agauuaggcc augucgccug aagaagagac     3360

cucccguuag gaaacggcca gccgcgacaa agaaagcugg gcaggccaag aaaaagaagu     3420

aaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu ccugccaaau     3480

aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                3575


<210>  3693
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3693
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcacccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaaa guaccacguu ggaauugacg uggguaccuu cagcguaggc cuggccgcga      180

uagaggugga ugacgcuggg augccaauca aaacacucuc ccuugucucu cacauucaug      240

auucaggacu ggacccggau gaaaucaaga gugccgugac uagguuggca agcuccggca      300

uugcccggcg cacgcgacgu uuauauagaa ggaagcggcg cagacugcag caacuagaca      360

aauuuaucca gaggcagggu uggcccguua uagagcucga agauuacucu gacccucugu      420

auccauggaa ggugcgggcu gagcuugcag ccucguacau cgcugaugaa aaagagcgcg      480

gggagaagcu gucagucgcc uugcgacaca uugcgagaca uaggggaugg cggaaccccu      540

augcaaaagu gagcagucuc uaccugccug acggcccauc cgaugccuuc aaggcuaucc      600

gugaagagau uaagcgcgcc ucuggccagc ccguaccuga aaccgcaaca guugggcaaa      660

uggugacucu gugcgagcuc ggaaccuuaa aacugagagg ugagggcggg guccuuagcg      720

cuagguugca gcaguccgac uaugcccggg aaauccagga gauaugucga augcaagaaa      780

uuggacagga gcuguaccgc aagaucauug augugguguu ugcggccgaa ucaccaaaag      840

gcagugcaag cucuagaguc gguaaggacc cgcuacagcc cgggaaaaau agggcucuca      900

aggccuccga cgcuuuccag cgguaccgca ucgccgcacu gaucggaaac cuuagaguua      960

ggguggaugg cgagaaacgu auucugucag uagaggaaaa gaauuuggug uuugaccacc     1020

ucgucaaccu gacaccuaag aaagagccag aaugggugac cauagccgag aucuuaggca     1080

uugaucgggg gcaacugauc ggaacugcua caaugaccga cgauggugaa cgagcgggcg     1140

caagaccccc uacgcacgac acuaauagga gcauaguuaa cagucgcauu gccccacucg     1200

uggauuggug gaagaccgcu ucugcccuug agcagcaugc aauggucaaa gcccugucca     1260

augcugaggu ggacgauuuc gacagccccg aaggggccaa gguacaggca uuuuucgcgg     1320

auuuggacga cgauguucac gcuaaacuag acucgcugca ucucccggug ggacgggccg     1380

cauauucuga ggauacacug gucagacuua cuaggcgcau gcuguccgac ggcguggauu     1440

uguacaccgc ccgacuccaa gaauuuggua ucgagccuuc auggacaccc ccaaccccuc     1500

ggaucgggga acccgucggc aacccagcug uggaccgugu ucugaagacg gugaguagau     1560

gguuagagag cgccacuaag acauggggag cacccgagag gguaauuauc gaacacgucc     1620

gcgagggcuu cgugaccgaa aaacgggcua gagagaugga uggggacaug aggcgacgcg     1680

ccgcgcggaa ugccaagcug uuucaggaga ugcaggaaaa acuaaacguu cagggaaagc     1740

cuuccagagc ugaucucugg agguaucaau cugugcagcg ucagaauugc cagugugcau     1800

acugcgguag cccaauuacu uucucaaaca gugagaugga ccacauaguc ccgcgcgccg     1860

gccaaggguc cacaaauacc cgggaaaacc ugguggcugu augucauaga ugcaaucaga     1920

gcaaaggaaa cacgcccuuu gccaucuggg caaagaacac cucuauugag ggcgugucag     1980

uuaaggaagc ggucgagagg acacgacacu gggugacuga uaccgguaug cggaguacag     2040

acuucaaaaa guucacuaaa gccguggucg agcgcuuuca gagagcuacc auggaugaag     2100

agaucgacgc caggagcaug gaauccguug cauggauggc uaaugagcuu cggucucgag     2160

uggcccagca uuucgcaucg cacgggacaa ccgugagagu auaucgcggc agcuugacug     2220

ccgaagcuag gcgugcgucc ggaaucucag gcaagcugaa auuuuucgac ggggucggaa     2280

agagucggcu cgauagaagg caucacgcca uugacgcagc ugugauagcc uuuacgucug     2340

auuacguugc cgagacacug gcugugcgca gcaaccuuaa gcaaucccag gcacaccgac     2400

aggaggcccc ucaguggaga gaguucaccg guaaagacgc ggaacaucgg gcugccugga     2460

gggucuggug ucaaaagaug gaaaaacugu cagcauugcu cacugaggau cugcgcgacg     2520

aucguguggu ugugaugagc aauguaagau uacggcuggg caacgggucu gcccacaagg     2580

aaaccaucgg aaaacuaucc aaggucaaac ucaguagcca gcugucugug uccgacauug     2640

auaaggcuuc aagugaggca cuuuggugcg ccuugacaag ggagccaggc uuugacccca     2700

aggaaggucu gccugcgaau ccagagcgcc auauccgagu gaacgggacc cacgucuaug     2760

cuggcgauaa uauuggacuc uuccccguua gcgccggcuc gaucgcacug agagggggau     2820

acgccgaauu aggcuccucu uuucaccaug cucgggugua caaaauaacu agcgguaaga     2880

aaccggccuu cgcaaugcug aggguauaua caaucgaccu uuugccuuac cgcaaccagg     2940

aucucuuuuc aguggagcug aagccacaaa cgaugaguau gagacaggcu gagaaaaagc     3000

uaagggacgc ccuggcgacc gggaaugccg aauaucucgg auggcugguc guggacgaug     3060

agcuuguugu ggacacuucc aagauugcaa cagaucaggu caaagcugug gaagccgagu     3120

ugggcaccau ccggcguugg cgcguugacg guuucuuuuc ucccagcaag cugcgauuaa     3180

gaccucucca gauguccaaa gaaggcauua agaaagaguc agcucccgag cugagcaaga     3240

ucauagauag gccagggugg cuuccugcag ugaacaagcu guucagugac ggaaauguca     3300

caguagugcg gcgcgauucu uugggcagag uuaggcucga auccacugcc caucugcccg     3360

ugaccuggaa aguccaaaag cggccagccg cuacgaaaaa ggcggggcag gccaaaaaga     3420

agaaauaagg acuagugucc accugucccu ccugggcugc uggauugucu cguuuuccug     3480

ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  3694
<211>  3281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3694
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcagc caggauucug gcguucgaca uaggaaucag cuccauuggu ugggcuuuuu      180

cugagaacga ugaacucaag gacugcggcg uucggaucuu caccaaagug gagaauccaa      240

agacagggga aucacuugcc cugccgcgcc gauuggcacg uagugccaga aagagguuag      300

cucggcgcaa agcaagacug aaccaucuaa agcaccucau ugccaaugag uuuaaacuga      360

acuacgaaga uuaucagagc uucgacgagu cccuugcuaa ggccuacaaa ggaucucuga      420

ucucgcccua ugaguugagg uuucgggcgc ucaaugaacu gcugucaaag caagauuucg      480

cacgcguaau acuccauauc gccaagcgaa gaggcuacga cgauauuaaa aacagcgacg      540

auaaggagaa aggugcuauc uuaaaggcca uuaaacagaa ugaagagaag cuggcaaacu      600

aucagagugu gggggaguac cuuuauaaag aauacuuuca gaaguucaag gaaaauucca      660

aagaauuuac uaacgucagg aauaagaaag agucuuacga acgguguauc gcucagagcu      720

ucuugaagga cgagcugaaa cuaauauuua agaagcaacg ugaguucgga uuuuccuucu      780

cuaaaaaguu cgaagaggaa gugcucagug uugccuuuua uaaacgcgcg cugaaggauu      840

ucagccaccu ugugggcaac ugcucauuuu ucacggacga gaaaagagcc ccuaaaaauu      900

ccccacuggc auuuauguuc gucgcuuuga ccaggauuau caaccuccug aauaacuuaa      960

agaacacaga aggcauucug uacacuaaag augaccucaa ugcccuucug aacgaggugu     1020

ugaagaaugg gacccuaaca uauaaacaga ccaagaaacu gcucggacug ucagacgauu     1080

acgaguuuaa gggugaaaag ggcacuuauu ucaucgaguu uaaaaaguac aaagaguuca     1140

ucaaggcucu uggggagcau aaccugagcc aggacgauuu gaaugaaauu gccaaagaca     1200

uaacacucau caaggaugag auuaaacuga agaaggcauu agccaaauau gaccugaacc     1260

aaaaucagau cgauagucua ucuaagcucg aguuuaaaga ccaccugaac auauccuuca     1320

aggcucuuaa auugguaacu ccccugaugc ucgaaggaaa gaaguacgau gaggcgugua     1380

augaacugaa ccuuaaaguu gcaauuaaug aggacaagaa agauuuucug ccugccuuca     1440

acgagacgua cuauaaggac gaagugacua auccagucgu guugcgggcu aucaaagagu     1500

accgaaaggu gcucaacgcc cuguuaaaga aauauggcaa gguccacaaa aucaauauug     1560

aacuggcacg cgagguuggu aagaaccaua gccagagagc caaaaucgaa aaggagcaga     1620

acgagaauua caaggcuaaa aaggacgccg aacuagagug cgaaaaacuc gggcugaaga     1680

uuaacucgaa aaauauacuu aaguugaggc uguucaaaga gcaaaaggaa uuuugugcau     1740

auucuggaga gaagaucaaa auuuccgauc uccaggacga gaagaugcug gaaaucgauc     1800

acaucuaccc cuauucacgg aguuucgacg auagcuacau gaacaaagug uuaguauuua     1860

ccaagcagaa ucaagagaaa cugaaccaga caccuuucga agcguuuggc aaugacuccg     1920

cuaaguggca gaagauugag gugcuugcca aaaacuugcc aacuaagaaa cagaagcgca     1980

uacucgauaa aaauuacaag gacaaggaac aaaagaacuu caaggauaga aaucugaacg     2040

acaccaggua uaucgcacgu cuaguccuga auuacacaaa agauuaucuc gacuuucugc     2100

cgcuuucuga ugacgagaac accaaguuga augacacgca gaaaggcagc aaggugcaug     2160

uugaggccaa gucagggaug cugaccagug cuuuacggca cacaugggga uucuccgcca     2220

aagaucgaaa caaccaccuc caucacgcaa uugacgcugu gaucauugcc uacgcgaaua     2280

acagcaucgu caaggccuuu ucugauuuca aaaaggaaca ggagucaaau agugcugaac     2340

uguaugccaa aaagauaagc gagcuugacu acaagaacaa aagaaaguuu uucgagcccu     2400

uuuccgguuu caggcagaaa gugcuggaua agaucgacga aauuuucgua ucuaaaccug     2460

agcgcaagaa accaucgggc gcauugcaug aagagaccuu ucggaaggaa gaggaguucu     2520

aucaaagcua cgggggaaag gaaggcguuc ucaaagcucu ggagcuaggu aagaucagaa     2580

aagugaaugg gaagauuguc aaaaacggcg auauguuuag gguggacauc uucaagcaca     2640

agaaaacuaa uaaguuuuac gccgucccca uauauacaau ggauuucgca cugaaagugc     2700

ucccuaacaa ggcgguugcc cgcuccaaaa agggagaaau uaaggacugg auccugaugg     2760

augagaauua cgaauuuugc uucucacuuu auaaagacag uuugauucug auccagacca     2820

aggauaugca ggagccagag uuuguguacu auaacgcuuu cacgucuagc accguauccu     2880

uaauagucuc aaaacaugac aauaaguuug aaacacucag caaaaaccag aagauccugu     2940

ucaagaaugc caacgagaaa gaagugauug caaagucuau cggcauucaa aaucuuaaag     3000

uuuucgagaa guacaucgug uccgcucugg gggaagucac uaaagccgag uuucgacagc     3060

gggaggacuu caagaaaaag cgucccgcag ccaccaagaa agcuggacag gcgaagaaaa     3120

agaaauaagg acuagugucc accugucccu ccugggcugc uggauugucu cguuuuccug     3180

ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3281


<210>  3695
<211>  3692
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  3695
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaag cgaccugguu cucggacuug auauugguau aggcuccgug gggguaggaa      180

uccugaacaa ggugaccggc gagauuaucc auaaaaauuc uaggauuuuc ccagccgcgc      240

aggcugaaaa caauuugguc cggcgcacaa accgacaagg ucguagauua gccaggcgga      300

agaagcaccg cagagugagg cugaaucggc uauuugagga aucagggcuc aucacugacu      360

ucacgaaaau aaguaucaac cugaauccgu accagcuucg cguuaaggga cugaccgaug      420

aguugagcaa cgaagaacuc uuuauugcac ugaaaaauau ggugaagcau cgaggcaucu      480

ccuaucugga cgaugccucu gacgauggca acucgucagu cggggacuac gcucagauug      540

ugaaagagaa uagcaagcag cucgaaacaa agacucccgg acaaauccag uuagagagau      600

aucagaccua cggucagcug aggggcgauu ucacaguaga aaaagacggg aagaaacacc      660

ggcuuauaaa cguuuuuccu accagugcau aucguuccga ggccuugcgc auucugcaaa      720

cucagcagga guucaaucca cagaucacag augaauuuau uaacagauac cuagagaucc      780

ucaccggaaa gaggaaauau uaccauggcc ccgguaauga aaagucucgg acggacuacg      840

ggcgauaucg cacuagcgga gagacccugg auaacaucuu cggcauucuu auaggcaaau      900

gcacauuuua cccugacgag uucagagcug ccaaggcguc cuauacugca caagaguuua      960

accuguugaa ugaccucaac aaucugaccg ugccaacaga gaccaagaaa uuaucaaagg     1020

aacagaaaaa ccagaucauu aauuacguca agaacgagaa agccaugggg cccgcuaagc     1080

uguucaagua uaucgccaaa cuccuuaguu gugauguggc agacauaaag ggauacagga     1140

uugauaaaag cgguaaggcu gaaauccaca cguuugaggc cuaucggaaa augaagacuc     1200

uggaaacauu ggacaucgag caaauggauc gcgagacccu agacaagcug gcguacgugc     1260

ucacucugaa uacagaaaga gagggcauuc aggaagcccu ugagcacgag uucgcagaug     1320

ggucuuucuc ccagaaacag gucgacgagc ugguucaauu uaggaaggcu aacucaagca     1380

ucuucggaaa aggcuggcau aauuuuagug ugaaguugau gauggagcuc auuccugaac     1440

uguacgagac cucugaagaa cagaugacga uauuaacccg ucuggguaaa cagaagacaa     1500

cuuccagcuc gaacaagacc aaauauaucg augagaagcu acucacagaa gagauuuaca     1560

auccaguagu ggccaaaucu guccggcagg cuaucaagau cgugaacgcc gcaauuaaag     1620

aauaugggga cuucgauaau auaguuaucg agauggcccg agaaacuaac gaggacgacg     1680

agaagaaagc uauucaaaag auccagaagg cgaauaaaga ugaaaaggac gcagccaugc     1740

ugaaagcugc caaccaguac aacggcaagg cagagcuucc gcacuccgug uuucauggac     1800

acaaacaguu ggccaccaag auuagacugu ggcaccaaca gggcgaaagg ugccucuaua     1860

cagggaagac caucucaaua caugaucuga ucaauaacag uaaucaguuc gaggucgacc     1920

acauucuucc ccugagcauc acuuuugaug acuccuuggc uaacaaagug cucguauacg     1980

ccacggcaaa ucaggaaaag ggacaacgca caccuuauca ggcgcuggau ucuauggacg     2040

augcuuggag cuuccgggag uuaaaagccu uuguuagaga gucaaagacc cugaguaaca     2100

aaaagaagga auaccuacuc acugaggaag acauuuccaa auucgaugug aggaagaaau     2160

uuaucgagcg caaucugguc gacacccgau acgcaagccg ggugguccuu aacgccuugc     2220

aggaacauuu ccgugcucac aagauagaua caaaaguguc uguugugaga ggucaauuua     2280

ccucacagcu gaggcgccau uggggcauug agaagacucg ggacacauau caccaccaug     2340

ccguagacgc acucaucauu gcugccagua gccagcugaa uuuauggaag aaacagaaga     2400

acacgcuggu cuccuacucu gaggaucaac uuuuggacau cgaaaccggg gagcucauau     2460

cggaugacga auauaaagag agcguguuca aggcgccaua ccagcacuuc guugauacuc     2520

ugaaauccaa ggaguuugaa gacucaaucc uauucaguua ucagguggau ucuaaauuua     2580

auagaaagau uagcgacgcc acaaucuacg cuaccaggca ggcaaagguc ggaaaagaua     2640

aggccgacga gacauaugug cugggcaaaa uuaaggauau cuacacucaa gacgguuacg     2700

augcuuucau gaaaauauau aagaaggaca aauccaaguu ucucauguac cgacaugacc     2760

cccagaccuu cgaaaaagua auugagccua uccuggaaaa cuauccaaau aagcagauca     2820

acgagaaagg gaaggaggug cccuguaauc cguuucuuaa guacaaagag gaacacggcu     2880

auauucgcaa guacagcaaa aagggaaacg gcccugaaau caaaucauug aaguauuacg     2940

auucuaagcu ggggaaccac auugacauaa cgcccaaaga uuccaauaac aagguugucu     3000

uacagagugu gagcccaugg cgggccgacg uguacuucaa uaaaaccaca ggaaaguaug     3060

agauccucgg ucugaaauac gcagaucuuc aauuugaaaa gggcacuggg accuauaaga     3120

uuucucagga gaaauacaac gacaucaaga aaaaggaggg agucgauucc gacucagagu     3180

ucaaauuuac acuguauaag aaugauuugc uccugguuaa agacacugaa accaaggaac     3240

agcaacuauu cagauuucug aguaggacga ugccuaagca gaaacauuac guggagcuca     3300

agcccuauga uaaacagaag uucgaaggcg gugaggcgcu gauuaaagug cuugggaacg     3360

uagccaauag cggccagugc aagaagggau ugggcaaauc gaacaucucc auauacaagg     3420

uccguacaga cgugcugggg aaucaacaca ucauuaaaaa cgagggagac aagccaaaau     3480

uagauuucaa gcgccccgcu gccaccaaga aagcaggcca ggcuaagaaa aagaaauaag     3540

gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu gccaaauaaa     3600

caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3692


<210>  3696
<211>  4250
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3696
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaac ccaguucgag ggauuuacaa accuguacca aguuagcaag acucucaggu      180

ucgaacuuau uccacagggu aaaacgcuga agcauauaca ggagcagggc uuuaucgaag      240

aggacaaggc ccggaaugau cacuauaaag aauugaagcc gauuaucgac cgcauuuaca      300

aaaccuaugc ggaucaaugc uuacagcugg ugcagcuaga cugggagaac cucuccgcug      360

ccaucgauuc uuaccgaaag gagaaaacag aagagacucg uaaugcacug auagaggaac      420

aggccaccua uagaaacgcu auccaugacu acuucauugg gaggacagau aaucuuaccg      480

acgcaaucaa caagcggcac gccgagauuu auaagggacu guuuaaagcu gaauuguuca      540

auggcaaggu acucaaacaa cuggguacug ugacaaccac ggagcaugaa aacgcccugc      600

uccgcucauu ugauaaguuc acuaccuacu uuaguggguu cuacgagaau aggaaaaacg      660

ucuuuagcgc ggaagacauc uccacagcaa uaccccacag aauugugcag gauaauuucc      720

cuaaguuuaa agagaacugu cacaucuuca cucgguuaau uaccgccguu ccaucucugc      780

gcgagcauuu cgaaaaugug aagaaggcua ucggaaucuu ugucucgaca ucaauugagg      840

aaguguucag cuuucccuuc uauaaccagc uuuugaccca gacgcaaaua gaccuguaca      900

aucagcuacu cggcggcauc agucgagagg ccgggacuga gaaaauuaag ggacugaacg      960

aaguacuuaa ccuggcaauc cagaaaaaug augagacagc ucacauaauu gccuccuugc     1020

cucauagauu uaucccacuc uucaagcaaa uccugucuga caggaacacc cuuagcuuua     1080

uucuggaaga guucaaaucc gacgaagagg uuauccaguc auuuugcaag uauaagacuc     1140

uccuucggaa ugaaaacgug cuggagacag cggaggccuu guucaaugaa cuaaacagua     1200

uugaucugac ccacauauuu aucagccaca aaaagcucga gacgauuucu uccgcacugu     1260

gugaccauug ggauacccuu cguaaugcuc uguacgaacg cagaaucuca gaguugacag     1320

guaaaaucac uaagagcgcc aaagagaagg uccagaggag ucucaagcac gaagacauua     1380

accugcagga gauaaucucu gcugccggca aagaauuauc cgaggcauuc aagcagaaaa     1440

ccagcgaaau ucugucgcau gcccacgcug cgcuagauca accccucccu acaacucuga     1500

agaaacagga ggagaaggaa auccuuaagu cucaguugga cucccugcuc gggcuguauc     1560

aucuucugga uugguuugca guggacgagu caaaugaagu ggauccagag uucagugccc     1620

gguugaccgg aauuaaacuc gaaauggagc cgagccuguc cuucuacaac aaggcucgaa     1680

auuaugccac uaaaaagccc uacucugucg agaaauuuaa guuaaacuuc caaaugccua     1740

cccuggcaag cggcugggac guuaauaaag aaaagaacaa uggugccauc cuauuuguga     1800

agaacgggcu cuacuaucug ggaauaaugc caaaacagaa gggccgcuac aaagcucuuu     1860

cauucgagcc cacugaaaag acgagugagg gcuuugauaa aauguauuac gacuauuucc     1920

cugacgccgc aaagaugauc ccaaagugcu ccacacaguu gaaagcggua accgcucacu     1980

uucagacuca caccacaccc auucugcuca gcaacaauuu caucgaaccg cuggagauua     2040

ccaaggagau cuacgauuua aacaauccug aaaaagagcc caagaaauuu caaacugccu     2100

augcaaagaa gacaggggac cagaaaggau acagagaagc ccuguguaag uggauagauu     2160

ucacgaggga cuuucuuucu aaauacacca agacuacauc aauugauuug aguagccucc     2220

ggccauccuc ucaguauaaa gaccugggug aguacuaugc ugagcuaaac ccucugcucu     2280

accauaucuc guuccagcgc auugccgaaa aggagaucau ggaugcagug gaaaccggca     2340

agcuguaucu uuuucaaaua uacaauaaag acuucgcuaa ggggcaccau ggaaaaccca     2400

acuugcacac acuguauugg acuggcuuau ucagcccaga gaaucucgcc aagaccucca     2460

ucaaacugaa cggucaggcg gaauuauuuu acagacccaa gucaaggaug aaacguaugg     2520

cccaccggcu gggggagaag auguugaaua agaaacucaa ggaucagaaa acgccuauuc     2580

cagacacccu guaccaagag cuauaugauu acgucaacca ucgacugagu cacgaccucu     2640

cugaugaagc uagagcacug cuuccgaaug ugaucacaaa ggagguuagc caugaaauua     2700

ucaaagacag gcgcuucacu uccgacaagu uuuucuuuca cgugcccaua accuugaacu     2760

aucaggccgc uaauucaccu agcaaguuca accagcgggu caaugccuac cugaaagagc     2820

auccagaaac acccauuauc ggcaucgaua gaggagagag gaacuuaauu uauaucacug     2880

ugauugacuc uaccggcaag auacucgagc agcgcucccu gaacacgauc caacaguuug     2940

auuaccagaa aaagcuggac aaucgagaaa aagagcgggu agcagcgcgu caggccugga     3000

guguuguggg gacaauuaag gaucuuaagc aaggauaucu aagccagguc auccacgaaa     3060

uuguggaccu caugauccac uaccaggcug ucgugguucu ggagaacuug aauuucgguu     3120

uuaaaucuaa gagaaccggc auagccgaaa aagcagugua ccagcaauuc gagaagaugc     3180

ugaucgauaa auuaaacugc cucguacuga aggacuaucc ugcugagaag gucgggggag     3240

ugcuuaaucc auaccagcug acugaucagu uuacauccuu cgccaaaaug ggcacccaau     3300

cagguuucuu guuuuauguu cccgcaccgu acacaaguaa gauugacccu cucaccgggu     3360

ucguggaucc auuugucugg aaaacaauca agaaccauga aagcaggaaa cacuuccugg     3420

agggcuuuga cuuccuacau uaugauguga agacgggaga cuuuauucug cacuucaaga     3480

ugaaucgcaa ccuuucguuu cagcggggcc uccccggguu caugccugcc ugggacaucg     3540

uauuugagaa aaaugaaacc caguucgaug cuaagggaac ucccuuuaua gcggguaaaa     3600

gaauugugcc aguuaucgag aaccauaggu ucacaggccg auaccgcgac cuguauccug     3660

ccaaugaauu gaucgcacug uuagaggaaa aggggauugu cuuccgggau ggauccaaca     3720

uacuccccaa acugcuugag aaugacgauu cucacgcuau cgacaccaug guggcccuga     3780

uuagaagcgu guugcagaug aggaacucaa augccgcuac aggcgaagau uacaucaaca     3840

guccaguccg ugaccucaac gguguuuguu uugauucccg cuuccaaaau ccggaguggc     3900

cuauggacgc agaugccaac ggggcguacc acauugcucu gaagggccag cuacugcuua     3960

aucaucucaa agagucuaag gaccugaagu ugcagaacgg aaucagcaau caggacuggu     4020

uagccuauau acaagaacug cggaacaaaa gacccgcagc cacuaagaaa gcuggccagg     4080

caaagaaaaa gaaguaagga cuagugucca ccugucccuc cugggcugcu ggauugucuc     4140

guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                4250


<210>  3697
<211>  4229
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  3697
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcaag cauuuaccag gaguucguua acaaguauuc ccugucuaaa acccucaggu      180

uugaacuuau accacaagga aagacacugg agaauaucaa ggcccggggu uugauuuuag      240

acgaugaaaa acgcgcgaag gacuacaaaa aggcuaaaca gaucauugau aaguaucauc      300

aguucuuuau cgaggaaaua cugucaagug ugugcaucag cgaggaccua cuccagaacu      360

acuccgaugu auauuucaag cugaaaaagu cugacgauga caaucuucaa aaagauuuua      420

agucggccaa agacacuauu aagaaacaga ucucagagua cauuaaggau agcgaaaagu      480

ucaaaaaccu guuuaaucag aacuugaucg acgcaaagaa aggccaggag agugaucuca      540

uacuguggcu gaagcaaucc aaagacaaug ggauugaacu cuucaaggcc aacucugaca      600

ucacggauau ugacgaggcu uuagagauca ucaagagcuu uaaaggaugg accacauauu      660

ucaagggcuu ucacgaaaau cguaaaaacg uguacuccuc aaaugauauu ccgacaagua      720

uaaucuaccg aauugucgac gauaaccugc ccaaguuccu ugagaauaaa gcaaaguaug      780

agagcuugaa ggacaaagcc ccugaggcua ucaacuacga acagauaaag aaagaucugg      840

ccgaggagcu aaccuuugac auugauuaua agacaucuga agugaaucag agaguuuucu      900

cccucgacga gguguucgaa aucgcgaacu uuaacaauua ccugaaccag ucagguauca      960

ccaaauucaa uacuauuauc gggggaaagu uugucaacgg cgagaauaca aagaggaaag     1020

gcauuaacga auauauaaau cuuuacagcc aacagaucaa cgauaagacc cugaaaaagu     1080

auaaaaugag uguguuguuc aagcagauuc ucucugacac ggaguccaag agcuuuguaa     1140

ucgacaaacu ggaggaugac ucggauguug ugacuaccau gcaaucuuuc uacgaacaga     1200

ucgcagccuu uaagacaguc gaggaaaaau ccauuaagga gacuuuauca cuccuguucg     1260

acgaucuuaa agcucagaag cuggacuuga guaaaauaua cuuuaagaau gauaagagcc     1320

uaaccgaccu gucccagcaa guguucgaug acuauucugu gaucgggaca gccguccucg     1380

aauacauuac ccagcagauc gcacccaaaa accuggauaa uccaagcaag aaagagcagg     1440

agcuuauugc uaagaaaacg gaaaaggcca aguaucuguc auuggagacu aucaaacucg     1500

cgcuggaaga guuuaacaag caucgggaca uagauaaaca augucgcuuc gaggaaaucu     1560

uagccaauuu ugcagcuauu ccuaugaucu ucgacgagau ugcccagaac aaggacaauc     1620

uggcucagau caguauaaaa uaccagaacc aaggaaagaa ggaucuacuc caggccuccg     1680

cagaagacga uguuaaagcc auuaaggacc ugcuugauca gacaaacaau uugcugcaca     1740

aacucaagau cuuccauauu agccagucug aggacaaagc uaacauccug gauaaggacg     1800

aacacuuuua ucuuguguuc gaggagugcu acuuugaacu ggcgaauaua guaccauugu     1860

auaacaaaau cagaaauuac auuacccaaa agcccuacuc agaugagaag uucaaacuca     1920

acuuugaaaa uaguacucug gcaaacgguu gggacaagaa uaaagagccu gauaacacag     1980

ccaucuuauu cauuaaggac gauaaauauu accugggcgu gaugaauaag aagaacaaua     2040

aaaucuuuga cgacaaggcu auaaaagaaa acaaggggga gggauauaaa aagauugucu     2100

acaagcuacu cccaggcgcc aauaaaaugc ugccgaaggu guucuuuagc gcaaaaucca     2160

ucaaguucua uaaccccucu gaggauaucc uuaggauucg gaaccacucg acccauacga     2220

aaaaugguag cccucagaag ggguacgaaa aguuugaguu caacaucgaa gacugucgca     2280

aauuuauuga uuucuauaag caguccauau caaaacaccc agaguggaag gacuucggau     2340

uucgauucag ugauacccaa agauacaauu cuaucgacga auuuuacagg gagguugaga     2400

accagggcua uaaauugaca uucgaaaaua uuagcgaguc cuacaucgau ucagugguca     2460

accagggcaa gcuguaucuc uuucagauuu acaauaagga cuucagcgcc uauucuaaag     2520

ggcggcccaa ccugcauacu uuauacugga aggcucuguu ugaugaacgu aaucuucaag     2580

acgugguaua uaaauugaac ggagaggccg aacucuucua ccgcaagcag uccaucccua     2640

aaaagauaac ccacccagca aaagaagcga ucgcuaauaa gaacaaggau aaucccaaaa     2700

aggagagugu uuuugaauac gaccugauua aagacaagag auucacagag gauaaauuuu     2760

ucuuccacug cccgaucacu auuaacuuua agagcucugg ugccaauaag uucaacgacg     2820

aaaucaaccu acugcucaaa gagaaggcaa augaugugca uauacugucc auugacaggg     2880

gcgagcggca ccuugccuau uacaccuugg ucgaugggaa aggaaacauc aucaagcagg     2940

acacauuuaa uauuauaggc aacgaucgaa ugaaaaccaa uuaucaugac aagcuggcug     3000

ccaucgaaaa ggaucgcgac ucagcaagaa aagauuggaa gaaaauuaac aauaucaagg     3060

agaugaaaga agguuacuua agucaggugg uccacgagau ugcuaagcuc gugaucgaau     3120

auaacgccau aguuguguuc gaggaccuga auuuuggguu caagaggggc cgguuuaaag     3180

uagagaagca agucuaccag aaacuugaaa agaugcugau cgagaaauug aacuaucucg     3240

uguucaagga uaaugaauuu gacaaaacug gaggcguucu gcgcgcguac cagcuaacgg     3300

ccccuuucga gacauuuaag aagaugggga aacagaccgg aauuaucuac uaugugcccg     3360

cugguuucac uagcaagauu uguccaguca ccggcuuugu gaaccaacug uacccuaaau     3420

augaaucggu auccaagucu caggaguucu ucagcaaauu ugacaagauc ugcuacaauc     3480

ucgauaaggg guauuucgag uuuucauucg acuacaaaaa cuuuggagau aaggcagcca     3540

aaggcaagug gacaauagcu aguuucgguu ccagacugau uaauuuuagg aacucugaca     3600

aaaaucauaa cugggauacc cgugaagugu aucccacuaa ggagcuugaa aaguugcuga     3660

aagacuacag caucgaguac gggcacggcg aguguaucaa ggccgcaauu ugcggagaau     3720

ccgauaaaaa guucuuugcg aaauuaacau caguucucaa cacgauccug cagaugcgga     3780

auagcaagac cggcacugag cuugacuauc ugauaagucc agucgccgau gugaacggga     3840

auuucuuuga cucucgacaa gcucccaaga acaugccuca ggaugccgac gcaaauggag     3900

cuuaccacau uggucuaaaa ggccucaugc uguuggggag aaucaagaac aaucaggaag     3960

gcaaaaagcu gaacuuagug auuaaaaaug aggaauauuu cgaguuuguc cagaacagga     4020

auaacaagcg cccagccgca acuaaaaagg ccggacaagc uaagaaaaag aaauaaggac     4080

uaguguccac cugucccucc ugggcugcug gauugucucg uuuuccugcc aaauaaacag     4140

gaucagcgcu uuacagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       4229


<210>  3698
<211>  51
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS2 variant

<400>  3698
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc c                51


<210>  3699
<211>  48
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS4 variant

<400>  3699
aagagacccg ccgcuaccaa aaaggccggc caggcgaaaa agaaaaag                    48


<210>  3700
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3700
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg aguauaaggu ucccucuaaa aaguucaaag ugcugggcaa uacggauaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug acuccggaga aaccgccgag      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuugc      240

uaucugcagg aaaucuucuc uaacgagaug gccaaagugg augacagcuu uuuccauaga      300

cuugaagagu ccuuucuggu cgaggaagau aagaaacacg agcggcaucc uaucuucggc      360

aauauugugg acgaaguugc cuaccacgag aaguauccga ccaucuacca ucuccgcaag      420

aaacuggugg auucuacaga caaggcugau cugagacuca uuuaucuggc ccuugcucac      480

augaucaaau uucgggggca cuuccugauu gagggagacc ucaaccccga uaauagcgac      540

gucgacaagc uguuuaucca acuugugcag accuacaacc agcuguucga agagaauccu      600

auuaacgccu ccggcguuga ugcgaaagcc auccucagcg cucgccuguc uaaguccaga      660

cggcuggaaa aucucaucgc ccagcugccc ggcgagaaaa agaacgggcu uuuuggaaau      720

cugauugcuc ucagccuggg ccuuacgccu aacuucaagu cuaauuuuga ccuggccgaa      780

gaugcgaaac uccaacuguc caaggacacc uacgaugacg aucuggacaa ccuccuggcc      840

cagaucgggg aucaguaugc ugaccuuuuc cuggccgcua aaaaccucag cgaugccauu      900

cugcuuucug acauccugcg cgugaauaca gagauuacca aggcuccccu cuccgcuagc      960

augaucaaaa gauacgacga gcaucaccag gaucugacac ugcucaaggc gcugguccgg     1020

caacagcuuc cugaaaagua uaaagagauc uucuuugacc aguccaagaa cggauacgcc     1080

ggcuauauug augggggagc uucucaggaa gaguucuaca aauuuaucaa gccgauucug     1140

gaaaagaugg acggcaccga ggagcuccug gugaagcuga auagagaaga ucuucuccgc     1200

aagcaacgga cguucgacaa cggcagcauc ccccaucaga uucaccuggg ggagcugcac     1260

gccauccuca gacgccagga agauuuuuau ccuuuccuga aagacaaucg ggagaagauc     1320

gaaaagauuc uuacauuuag aauccccuac uacgugggac cucuggcccg cggcaacucc     1380

cgguucgcuu ggaugaccag aaagagcgag gagacaauua cccccuggaa uuuugaagag     1440

gucguugaua aaggggccuc ugcgcaaucc uucaucgaac gcaugaccaa cuuugacaag     1500

aaucucccga acgagaaggu gcugccuaaa cauagccugc uuuaugagua cuucacagug     1560

uauaaugaac ucacgaaggu caaauacgug accgagggaa ugcggaagcc cgcuuuucug     1620

ucuggcgaac agaaaaaggc cauuguugau cugcuguuca aaacaaacag aaaggugacc     1680

gucaagcagc ugaaagagga cuauuucaag aaaaucgaau guuuugauag cguggagauu     1740

uccgggguug aggaccgcuu caaugccucu cuuggaacau accacgaccu gcucaagauc     1800

aucaaagaua aggacuuucu ggauaacgaa gagaaugaag acauucugga ggauaucgug     1860

cuuacccuca cgcuguucga agaccgggag augauugagg aaagacugaa gacauaugcu     1920

caucucuuug augacaaagu caugaagcag cugaaacggc gcagauacac cggcuggggc     1980

cggcuuagca gaaagcugau caacgggauu cgcgauaaac aauccggaaa gaccauccuc     2040

gacuuccuca agagcgaugg cuuugccaac cggaauuuca ugcagcugau ccacgacgau     2100

ucucuuacau uuaaagagga cauucagaag gcucaggugu ccgggcaagg agacagccuc     2160

caugaacaca ucgccaaccu ggcgggcucu ccugccauua aaaaggggau ccugcagacc     2220

gucaaagugg uugaugagcu cgugaagguc augggcagac acaagcccga aaauauugug     2280

aucgagaugg cucgcgagaa ccagacaacg cagaaaggac aaaagaacuc cagagaacgg     2340

augaaacgca ucgaggaagg cauuaaggag cuggggagcc agauccuuaa agagcauccc     2400

guugaaaaua cccagcugca gaaugagaag cucuaccugu auuaccugca aaacggaaga     2460

gacauguaug uggaucagga acuugacauu aaucggcugu ccgauuacga cgucgaucac     2520

aucgugccuc agucuuuccu caaagacgau agcauugaca acaaggugcu gacacgcucc     2580

gauaagaaua gaggcaaauc ugacaacguu ccgagcgagg aagucgugaa gaaaaugaag     2640

aauuauuggc ggcaacuccu gaacgccaaa cuuaucaccc agcgcaaguu ugacaaucug     2700

acaaaggcug agagaggggg acucuccgag cuggauaaag ccggcuucau caagcggcag     2760

cugguggaaa cccgccagau uacgaaacau gucgcgcaaa uccuugacag cagaaugaac     2820

accaaguacg augagaacga caaacugauu cgggaaguga agguuaucac acucaagucu     2880

aaacuggugu ccgauuuuag aaaggacuuc caguucuaua aaguccgcga gauuaauaac     2940

uaccaccacg cccaugaugc uuaccucaau gccgugguug ggaccgcucu gaucaagaaa     3000

uaucccaaac uugaaagcga guuuguguac ggcgacuaua aggucuacga ugugcggaaa     3060

augauugcca agucugagca ggaaaucgga aaagccacag cuaaguauuu cuuuuacucc     3120

aacaucauga auuucuuuaa aaccgagauu acgcuggcga acggcgaaau cagaaagcgc     3180

ccucucauug agacaaaugg ggaaaccgga gagaucguuu gggacaaggg ccgggauuuc     3240

gccacaguga gaaaaguccu gagcaugccc caggugaaca uugugaagaa aaccgagguc     3300

caaaccgggg gauuuucuaa ggaaagcauc cugccuaaac gcaauuccga caaacuuauc     3360

gcucggaaaa aggauuggga ccccaagaaa uauggcgggu ucgacagccc gacaguugcc     3420

uacucugugc ugguggucgc caagguggag aaaggcaagu ccaaaaagcu caagagcguu     3480

aaagaacugc ucggaauuac gaucauggag agauccucuu uugagaagaa cccuauugau     3540

uuccuggaag cuaaaggcua caaggaggug aaaaaggacc uuaucauuaa gcugcccaaa     3600

uauagccugu uugaacucga gaaugggcgc aagcggaugc uggccuccgc gggagaacuu     3660

cagaaaggca acgagcuggc ucucccuucu aaguacguca auuuccugua ucucgccagc     3720

cacuacgaga aacugaaggg gucccccgaa gauaacgagc agaagcagcu uuucguggaa     3780

caacauaaac acuaucugga cgagaucauc gaacagauuu cugaguuuag caagagaguu     3840

auccuggccg augcuaaucu cgacaaagug cuguccgccu acaacaagca ucgggauaaa     3900

ccuauucgcg agcaggcgga aaacaucauu caccuuuuca cccugacaaa ucucggagcu     3960

cccgccgccu uuaaguauuu cgacaccaca aucgauagaa aacgguacac cagcacgaag     4020

gagguccugg acgcuacacu cauucaccaa ucuaucaccg gccuguacga aacccgcauc     4080

gaucuuuccc agcucggggg cgacuga                                         4107


<210>  3701
<211>  4890
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3701
augaacuuca agauccugcc cauugccauc gaccucggcg ugaaaaauac cggggucuuu       60

agcgcuuucu accagaaggg aacaucccug gagagacuug auaacaaaaa uggcaagguu      120

uaugaacugu cuaaagacag cuacacgcuc cugaugaaca aucggaccgc ccgcagacac      180

caacggcgcg ggauugauag aaagcagcuu gugaagcggc uguuuaaacu caucuggaca      240

gagcagcuga accuggaaug ggacaaggau acccagcaag cgauuuccuu ccucuuuaau      300

cgcagaggau ucucuuuuau cacagacggc uauagcccug aguaccugaa caucgugccg      360

gaacagguca aagcuauucu uauggauauc uucgacgauu auaaugggga ggacgaucug      420

gacuccuacc ucaagcuggc caccgagcag gaaucuaaaa uuagcgagau cuauaacaaa      480

cuuaugcaga agauucugga auuuaaacuc augaagcugu gcacagauau caaagacgac      540

aaggugucca ccaaaacgcu gaaggagauu accagcuacg aguucgaacu ccuggccgau      600

uaccuugcua auuauucuga gucccugaaa acacaaaagu uuagcuacac cgacaagcag      660

ggaaaccuca aagaacuguc uuauuaccau cacgauaagu auaauaucca ggaguuccuu      720

aaacggcaug ccacaaucaa cgaccgcauu cuggauaccc uccugacgga cgaucuggac      780

aucuggaauu uuaacuucga aaaguucgau uuugacaaaa acgaggagaa gcuccagaau      840

caagaagaua aggaccacau ucaggcucau cugcaccacu ucguuuuugc cgugaacaaa      900

aucaaguccg agauggcgag cggcggcaga caucggucuc aguacuucca agaaauuaca      960

aauguccuug acgagaacaa ucaccaggaa ggguaucuga aaaacuuuug ugagaaucuc     1020

cauaacaaga aauacuccaa ucugagcgug aagaaccuug uuaaucugau cggaaaccuc     1080

uccaaucugg agcugaagcc ccuccgcaaa uacuucaacg auaagaucca cgccaaagcu     1140

gaccacuggg augaacagaa guuuaccgag acauauugcc auuggauucu gggcgaaugg     1200

agaguggggg ucaaagacca ggauaagaag gacggagcca aauacucuua uaaggaucuu     1260

uguaaugagc ugaaacaaaa ggugaccaaa gcuggccucg uggacuuccu gcuggaacuu     1320

gauccuugcc ggaccauucc cccuuaccuc gacaacaaca aucgcaagcc cccuaaaugu     1380

cagagccuga uccugaaccc gaaguuucuc gauaaucagu aucccaacug gcagcaauac     1440

cugcaggagc uuaagaaacu gcaguccauc cagaauuauc ucgacagcuu cgagacagau     1500

cugaaggucc ugaaaucuuc caaggaccaa ccuuacuuug uugaauacaa aagcucuaac     1560

cagcagauug ccagcgggca gagagacuau aaggaucuug acgcgcggau ccuccaauuc     1620

aucuuugaua gagugaaggc cuccgacgag cugcugcuca augaaauuua cuuccaggcu     1680

aaaaagcuga aacagaaggc cucuagcgag cuugagaaac uggaauccag caagaagcuc     1740

gaugagguga ucgcuaacuc ucaacugucc cagauucuga aaagccagca cacgaaugga     1800

aucuucgaac agggcaccuu ucuucaucuc gucugcaagu auuacaaaca acgccagcgg     1860

gccagagacu cucgccugua uauuaugccc gaguaccggu augauaagaa acugcacaag     1920

uacaacaaua caggcagauu cgacgaugac aaccagcucc ugaccuacug uaaucauaag     1980

ccucgccaga aacgguauca acuucugaac gaucucgccg gggugcugca gguuuccccc     2040

aauuuucuga aggacaaaau cggaagcgau gacgaccuuu ucauuuccaa guggcucgug     2100

gaacacauca gaggcuuuaa aaaggcuugc gaggauucuc ugaaaaucca gaaggacaac     2160

cgcgggcugc ucaaccacaa gauuaauauc gcgcggaaca caaaaggaaa gugugagaaa     2220

gaaauuuuca aucugaucug caagauugag ggcagcgaag auaaaaaggg gaacuacaag     2280

cauggacuug ccuaugagcu gggcguccuc cuguuuggcg aaccgaauga ggcuuccaaa     2340

ccugaguucg acagaaagau caaaaaguuu aacucuaucu acagcuucgc ccagauucaa     2400

cagaucgccu uugcugaacg caaagggaau gccaacaccu gugcggugug cuccgcugau     2460

aaugcccacc ggaugcagca aauuaagauc acggagcccg uugaagacaa caaggauaaa     2520

auuauccuga gcgccaaggc ucagagacuu ccugccaucc ccacacggau uguggacgga     2580

gcugucaaaa agauggccac cauccuggcg aaaaauauug uggaugacaa cuggcagaau     2640

aucaagcagg uccucucugc caagcaucaa cugcacauuc ccaucaucac cgaguccaac     2700

gcuuucgaau uugagccugc ccucgcugau gugaaaggca agagccugaa agaccgcaga     2760

aagaaagccc uugagcggau uucuccggaa aauaucuuca aggacaagaa caacagaauu     2820

aaagaguucg cgaaggggau cuccgccuau agcggagcua aucugacaga uggcgacuuu     2880

gauggggcca aagaggaacu cgaccacauu aucccccgcu cucauaagaa auacggcacc     2940

cugaacgaug aggcuaaucu gauuugcguu acacggggag acaacaagaa uaaaggcaac     3000

agaaucuucu gucuucgcga ucuggccgac aauuauaagc ucaagcaguu ugaaacgacc     3060

gaugaccugg agaucgaaaa gaagauugcc gauacaaucu gggacgcuaa caaaaaggau     3120

uucaaauuug ggaauuacag aucuuucauu aaccucaccc cucaggagca gaaggcguuu     3180

cggcacgccc uguuccuugc ugacgaaaau cccaucaagc aagccgugau ucgcgccauc     3240

aacaauagaa accggacauu ugucaaugga acccagcgcu acuucgcuga ggugcuggcc     3300

aacaauaucu aucucagagc gaaaaaggag aaccugaaca cggacaaaau uuccuuugau     3360

uacuucggca ucccuaccau ugggaaugga cggggcaucg cugaaauucg ccagcuguau     3420

gagaagguug acagcgauau ccaggccuac gccaaagggg acaagcccca agcuagcuau     3480

ucccaucuua ucgaugccau gcuggcguuc ugcauugcug ccgacgaaca cagaaacgau     3540

ggcagcaucg gacucgagau ugacaagaau uacucccugu auccgcucga uaaaaacaca     3600

ggcgaagugu uuaccaagga caucuucucu cagauuaaaa ucacagauaa ugaguuuagc     3660

gacaagaaac ugguccggaa gaaggccauu gagggguuca acacccaucg ccagaugacg     3720

agagauggaa ucuacgcuga aaauuaccuu ccuauccuga uucacaaaga gcugaacgaa     3780

gugcggaagg gcuauacaug gaaaaauucc gaggaaauca agauuuuuaa agggaagaaa     3840

uacgacaucc agcaacucaa caaucuggug uauugucuua aguucguuga caagcccauu     3900

ucuaucgaua uccagauuag cacccuggag gagcucagaa acauccugac aaccaauaac     3960

auugccgcua ccgccgaaua cuauuacauc aaucucaaaa cacagaagcu gcacgaguau     4020

uacauugaaa acuacaacac ggcgcuugga uauaaaaagu acuccaaaga gauggaguuu     4080

cugcgcucuc uggccuaucg gagcgaaaga gucaagauca aguccaucga cgaugugaaa     4140

caagugcucg acaaggauag caauuucauu aucggcaaaa uuacccugcc uuuuaagaaa     4200

gaguggcagc gccuuuaccg ggaauggcag aacacaacca ucaaggacga uuaugaguuc     4260

cugaaguccu uuuucaaugu caaaucuauu acaaagcucc auaaaaaggu gagaaaagac     4320

uuuagccugc ccaucucuac caacgaaggg aaguuccucg uuaagcgcaa aacgugggau     4380

aauaacuuca uuuaccagau ccugaaugac uccgauagcc gggcugacgg cacaaagccu     4440

uuuauccccg ccuucgacau uucuaaaaac gagaucgugg aggcuauuau cgauuccuuu     4500

accagcaaga auauuuucug gcuuccgaaa aacaucgaac ugcaaaaggu cgacaauaaa     4560

aacaucuuug ccauugauac cuccaagugg uucgaggugg aaacaccuag cgaccugaga     4620

gauaucggaa uugccaccau ccaguauaag auugacaaua acucucgccc caaaguucgg     4680

gugaagcucg auuacgucau cgacgaugac agcaaaauca auuacuuuau gaaccacucc     4740

cugcuuaagu cuagauaucc cgauaaagug cuggagauuc ucaagcagag cacaaucauu     4800

gaguucgaau ccucuggcuu uaacaagacg aucaaagaga ugcuggggau gaagcucgcu     4860

ggaauuuaca augaaaccag caacaauuga                                      4890


<210>  3702
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3702
augaagagaa acuacauccu gggccucgac auugggauca ccagcguggg auauggcauu       60

aucgauuacg agacacggga cgucauugau gccgggguuc gccuguucaa agaagcuaau      120

guggagaaca augaaggaag acgguccaag cgcggcgcca gacggcuuaa acgcagacgg      180

cgccacagaa uccagcgggu gaagaaacug cucuuugacu auaaccugcu uacggaucau      240

ucugagcuga gcgggaucaa ucccuacgaa gcgcgcguca agggacucuc ccaaaagcug      300

ucugaggagg aguucagcgc ugcccugcuc caccuggcca aaagacgggg cgugcauaac      360

guuaaugaag uggaagagga caccggcaac gagcuuucca caaaggaaca gauuucucgc      420

aauagcaaag cucuggagga aaaguauguc gccgagcucc agcuggaaag acuuaaaaag      480

gauggggagg ugcggggauc caucaacaga uuuaagacca gcgacuacgu uaaagaggcu      540

aagcagcugc ucaaagugca aaaggccuau caccagcugg aucagucuuu cauugacaca      600

uacaucgauc ugcucgaaac ccgccggaca uacuaugagg gcccugggga aggauccccg      660

uuuggcugga aagacauuaa ggagugguac gaaaugcuga uggggcauug caccuauuuc      720

cccgaggagc uuagaagcgu caaauacgcg uauaaugccg aucuguacaa cgcucucaau      780

gaccugaaca aucuugugau cacgcgcgac gaaaacgaga agcuggaaua uuacgagaag      840

uuucagauua ucgaaaacgu guucaaacaa aagaaaaagc cuacccucaa acagaucgcc      900

aaggagauuc uggucaauga ggaagauauc aagggauacc ggguuacauc uaccggcaaa      960

cccgaguuua caaaccugaa gguguaucac gacauuaaag auaucaccgc uagaaaggaa     1020

auuaucgaga augccgagcu ccuggaccag aucgcgaaaa uucuuacgau cuaccagucc     1080

agcgaagaua uucaagagga acugacaaac cucaauucug agcugaccca ggaagagauc     1140

gagcagauuu ccaaccuuaa gggcuauaca gggacccaca aucugagccu caaggccauc     1200

aaccugaucc uggacgaacu cuggcauacc aaugauaacc aaauugcuau cuucaaucgc     1260

cugaaacuug ugccuaagaa agucgaccug ucccagcaga aggagauucc cacaacgcuc     1320

guggaugacu uuauccuguc uccuguugug aaacggagcu ucauucaguc caucaagguc     1380

auuaacgcca ucaucaagaa auacggacug ccgaaugaua uuaucauuga acuugcuaga     1440

gagaagaaca gcaaagacgc ccaaaagaug aucaaugaaa ugcagaaacg caaccggcag     1500

accaacgaga gaauugagga aaucauccgc acaaccggca aggagaaugc caaauaucuc     1560

auugaaaaga ucaagcugca cgacaugcag gaggggaaau gucuguacuc ucucgaagcu     1620

auuccccugg aggaucuucu gaacaauccu uuuaacuaug agguggacca uaucauuccc     1680

cgguccguua gcuucgauaa uucuuucaac aauaaggugc ucgucaaaca agaagagaac     1740

agcaagaaag gaaauagaac accuuuucag uaccuguccu cuagcgacuc caagaucagc     1800

uacgaaaccu ucaagaaaca cauccugaac cuugcgaagg gcaaagggcg gauuucuaag     1860

acgaaaaagg aguaucuccu ggaggaacgc gauaucaaua gauuuuccgu gcagaaggac     1920

uucauuaacc ggaaucuggu cgauacaaga uacgccaccc gcggacucau gaaccugcuu     1980

cggagcuauu uuagagugaa uaaccuggac guuaaaguga agucuaucaa cggcggcuuc     2040

accuccuuuc uccgcagaaa auggaaguuc aaaaaggagc ggaauaaggg guacaaacac     2100

caugcugaag augcccugau uaucgccaac gcugacuuua ucuucaagga guggaaaaag     2160

cuggauaaag ccaagaaagu cauggaaaau cagauguuug aggagaagca agcggaaagc     2220

augcccgaga uugaaacaga gcaggaauau aaggagaucu ucauuacccc gcaccagauc     2280

aaacauauua aggacuuuaa agauuacaag uauucccacc gcguggacaa aaagccuaac     2340

agagagcuua ucaaugacac acucuacucu acgcggaagg augacaaagg aaacacccug     2400

auuguuaaua accugaaugg ccucuacgau aaggacaacg auaaacugaa gaaacuuauc     2460

aauaagagcc ccgaaaagcu gcucauguau caucacgacc cucagacaua ccaaaaacug     2520

aagcugauca uggagcagua uggggaugag aaaaaccccc uuuacaagua uuacgaagaa     2580

accggaaauu aucucacaaa auacuccaag aaggacaacg gccccgugau uaaaaagauc     2640

aaauacuaug ggaauaagcu gaacgcucac cuggauauua ccgacgauua cccuaacucu     2700

cgcaauaaag ucgugaagcu cagccugaag ccguauagau ucgacgugua ccuugauaac     2760

ggcguuuaua aauucgucac ggugaagaau cuggacguga ucaaaaagga gaacuacuau     2820

gaggucaauu ccaaaugcua cgaagaggcc aagaaacuca agaagauuag caaccaggcc     2880

gaauuuaucg cuucuuucua caauaacgac cugaucaaaa uuaauggaga gcuguaucgg     2940

gugaucggcg uuaacaauga ucuucugaac cgcauugagg ugaauaugau cgacauuacc     3000

uacagagaau aucucgagaa caugaaugau aagcggcccc cucgcaucau caaaacaauu     3060

gccuccaaga cccaaagcau caaaaaguac ucuacagaca uucuggggaa ccucuaugaa     3120

gucaagucca aaaagcaucc ccagaucauu aaaaagggau ga                        3162


<210>  3703
<211>  4230
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3703
augcuguuca acaagugcau cauuaucagc auuaaucucg acuuuuccaa caaagagaag       60

uguaugacca aacccuacuc uaucggccug gauauuggga caaauagcgu gggaugggcc      120

gucaucacgg acaacuauaa gguuccuucc aaaaagauga aggugcuugg caauaccucu      180

aaaaaguaca ucaaaaagaa ccugcucggg gugcugcuuu ucgauagcgg aauuacagcu      240

gaaggcagac ggcugaaacg caccgccaga cggcgcuaua caagacggcg caauagaauc      300

cucuaccugc aggagauuuu uagcaccgaa auggcgacac uggacgaugc uuucuuucaa      360

cggcucgacg auucuuuccu ggucccggac gauaagcgcg acagcaagua ucccaucuuu      420

gggaaccuug uggaggaaaa aguuuaccac gaugaguucc cuaccauuua ucaucugaga      480

aaguaccucg ccgacuccac gaaaaaggcc gaucugcggc uuguguaccu ggcucucgcc      540

cacaugauca aauaucgcgg acauuuucug auugagggcg aguucaauag caagaacaau      600

gacauccaga aaaacuuuca ggacuuccug gauaccuaca augcuaucuu ugaaucugac      660

cucucccugg aaaacagcaa gcagcuugag gagauuguca aggauaaaau cucuaagcug      720

gaaaagaagg accggauucu caaacuguuc cccggcgaga aaaauuccgg gaucuucagc      780

gaauuucuua agcugauugu gggaaaccaa gccgauuuca gaaaaugcuu uaaccucgac      840

gagaaggcgu cucugcacuu cuccaaagaa agcuaugaug aggaccugga gacacuccug      900

ggcuacaucg gggaugacua uuccgauguu uuucuuaagg ccaaaaagcu guacgacgcu      960

auccuccugu cuggauuccu uaccgugaca gacaaugaaa ccgaggcccc ucugagcucc     1020

gcuaugauua agcgcuauaa cgaacauaaa gaggaucucg cccugcugaa ggaauacauc     1080

agaaauauua gccucaaaac guacaacgag gucuuuaagg acgauacaaa gaauggcuau     1140

gcgggguaca ucgacggaaa gaccaaccag gaggauuucu auguguaccu gaagaaucuu     1200

cuggccgaau uugagggcgc ugacuauuuc cucgaaaaga uugaucggga ggacuuucug     1260

agaaagcagc gcacauucga uaacggcucu auccccuacc agauccaucu gcaagaaaug     1320

cgggccauuc uugacaaaca ggcuaaguuu uauccuuucc ucgccaaaaa uaaggagaga     1380

aucgagaaaa uucugaccuu ccgcaucccg uacuacgugg ggccccuggc ccggggaaac     1440

uccgauuuug cuuggagcau uagaaagcgc aaugaaaaga ucaccccuug gaacuucgag     1500

gacgucauug auaaagaauc uagcgcggag gccuuuauca aucggaugac auccuucgac     1560

cucuaucugc ccgaggaaaa gguucuuccu aaacacucuc ugcucuacga gacguuuaac     1620

guguauaaug aacugaccaa ggugagauuc aucgcugaga gcaugcgcga cuaccaguuu     1680

cuggauucca aacagaagaa ggacauuguc cggcuuuauu ucaaagauaa gagaaaagug     1740

acagacaagg auaucauuga auaccuccac gccaucuaug gcuacgacgg gauugagcug     1800

aaaggaaucg agaagcaauu uaacagcucu cuguccaccu accacgaucu ccugaacauc     1860

auuaaugaca aggaguuccu ugaugacagc ucuaacgaag ccaucauuga agagaucauu     1920

cauacacuga ccaucuuuga agaucgcgag augaucaaac agcggcucuc caaguucgag     1980

aauauuuuug acaaaagcgu ucugaagaaa cuguccagac ggcacuauac gggcuggggg     2040

aaacuuucug cuaagcucau caacggcauu cgcgaugaaa aauccggaaa uacaauccug     2100

gacuaccuga uugacgaugg caucuccaac agaaauuuca ugcagcucau ccacgacgau     2160

gcccugucuu ucaagaaaaa gauucaaaaa gcgcagauca uuggggacga ggauaaggga     2220

aacaucaaag aaguggucaa gagccuuccc ggcuccccgg cuauuaagaa agggauccug     2280

cagagcauua agaucgugga cgagcucguu aaagugaugg gaggccggaa gccugaaucu     2340

aucgucgugg agauggccag agagaaucag uauaccaacc aagggaaauc caauagccag     2400

cagcgccuga agcggcugga aaagucucuu aaagagcucg gcuccaagau ucugaaagaa     2460

aacauccccg ccaagcugag caaaauugau aauaacgcuc uccagaauga cagacuguac     2520

cuuuauuacc ugcaaaacgg aaaggauaug uauaccggcg acgaucucga caucgaccgc     2580

cugucuaacu acgauauuga ccauaucauc ccucaggccu uucugaagga uaauagcauu     2640

gacaacaaag uccuuguguc cagcgcuucu aauagaggga aguccgauga cuuccccagc     2700

cuggagguug ugaaaaagcg gaaaacauuu ugguaccagc uccugaaguc caagcucauc     2760

ucucagcgca aauucgauaa ccugaccaag gccgagagag gaggccuucu gcccgaagac     2820

aaagcggggu uuauucaacg gcagcucguc gagacacgcc agaucacgaa gcacguggcc     2880

agacugcugg augaaaaauu caauaacaag aaggacgaga auaaccgggc uguucgcacc     2940

gugaaaauua ucacacuuaa gagcacccug gucucccagu uuagaaaaga uuucgaacuc     3000

uauaaggugc gggagaucaa ugacuuucau cacgcccaug augcuuaccu gaacgccgug     3060

auugcgucug cccuccugaa aaaguauccu aaacuugagc cggaguucgu uuacggagac     3120

uaucccaagu acaauagcuu ucgcgaaaga aaguccgcua cagaaaaagu cuauuucuac     3180

ucuaacauca ugaauauuuu caagaaaagc aucucccugg ccgacggccg ggugauugag     3240

agaccucuca ucgaagugaa cgaggagacc ggggaaagcg ucuggaauaa ggagucugau     3300

cuggcuacgg ugcgccgggu ucuguccuac ccccaaguga acgucgugaa aaagguugag     3360

gaacagaacc acggccuuga cagaggaaag ccuaaaggcc uguuuaaugc caaccucagc     3420

ucuaagccca aaccuaauuc caacgaaaau cugguggggg ccaaggagua ucucgauccg     3480

aaaaaguacg gaggcuaugc ugggauuagc aacucuuucg cgguccuggu gaagggaacc     3540

aucgagaaag gcgccaagaa aaagaucaca aauguucuug aauuucaggg gauuuccauc     3600

cuggaccgca uuaacuaccg gaaagauaag cugaauuucc uccuggagaa gggcuauaaa     3660

gacaucgaac uuauuaucga gcugcccaag uacagccucu uugagcuguc cgauggaagc     3720

agacgcaugc ucgcuucuau ccugagcacc aacaauaaac ggggcgaaau ucacaagggg     3780

aaccaaaucu uccuuuccca gaaauuugug aagcugcugu aucaugccaa aagaauuucu     3840

aauacaauca acgagaauca ccgcaaguac gucgaaaacc auaagaaaga guucgaggaa     3900

cucuuuuacu auauucugga guucaaugaa aacuacgugg gagccaagaa aaacggcaaa     3960

cuucugaaua gcgcuuuuca guccuggcag aaccacucua ucgacgagcu cuguagcucc     4020

uucaucgggc cuaccggauc ugaacggaaa ggccuguuug agcucacgag cagagggucc     4080

gccgcggauu ucgaguuccu gggcgugaag auuccccggu aucgcgacua cacaccuagc     4140

ucucuucuga aggaugcuac ccugauccac caauccguca caggacucua ugaaaccaga     4200

auugaccugg ccaaacuugg cgagggguga                                      4230


<210>  3704
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3704
augugguacg ccagccugau guccgcucac caucucagag ugggcaucga cgucgggacc       60

cacucuguug gacuggccac acuucgggug gaugaccaug gcacgcccau ugagcugcuc      120

agcgcgcugu cccacaucca ugauucuggg gugggaaagg aaggcaaaaa ggaccacgau      180

acccgcaaaa agcugagcgg gauugcuaga cgggcccgca gacugcucca ccaucggcgc      240

acacagcugc aacagcugga cgagguccuc agagaucugg gauucccuau cccgaccccc      300

ggcgaauuuc uugaccugaa cgagcagaca gauccuuauc ggguguggcg cguuagagcc      360

cggcucgugg aagagaaacu gcccgaggaa cuucgcggcc cugcuauuuc cauggccguc      420

agacacaucg cucggcaucg cggguggaga aaucccuacu cuaaggugga gagccugcuc      480

uccccugccg aagagagccc guucaugaag gcgcugcggg agagaauccu ggccaccaca      540

ggagaaguuc ucgacgaugg cauuaccccc gggcaggcua uggcccaagu ggcucugacg      600

cacaacaucu cuaugcgcgg accugagggc auucuuggga aacugcacca guccgacaau      660

gccaacgaaa uccggaagau uugcgcgaga cagggaguca gccccgaugu guguaaacag      720

cuccugcgcg ccguguuuaa ggcugacucu ccucggggcu ccgccgucag cagaguugcu      780

cccgacccgc uuccuggcca agggucuuuc cgccgggccc ccaaaugcga uccugaguuu      840

cagagauucc gcaucauuuc caucguggcc aaucugcgga ucagcgaaac caagggagag      900

aacagacccc ucacagcuga cgagcgccgg caugugguca ccuuucugac agaagauucc      960

caggcggacc ugaccugggu ggauguugcc gagaagcucg gcgugcacag acgggaccug     1020

cgcgggacgg cuguccauac agaugacgga gaaagaucug ccgcccggcc cccuauugau     1080

gcuaccgaca gaaucaugcg ccagacaaaa auuagcuccc uuaagaccug gugggaggaa     1140

gccgauagcg agcaacgggg cgcgaugauc agauaucugu acgaggaccc gaccgacucu     1200

gaaugugcug agauuaucgc cgaacucccc gaggaagauc aggccaaacu ggacucccuu     1260

caccuuccug cugggcgcgc cgcuuauagc agagagucuc ucacagcccu gagcgaucau     1320

augcuggcga cgaccgacga ucuccacgag gcccggaagc gccuguucgg aguggacgau     1380

uccugggcuc ccccugccga agcuaucaau gcccccguug gcaacccguc uguggacaga     1440

acacuuaaaa uugucggccg guaccugagc gcgguggagu ccaugugggg gaccccugaa     1500

gucauccacg uggagcaugu ucgcgaugga uuuacaagcg agagaauggc cgacgaacgg     1560

gauaaggcua aucgcagacg guauaacgac aaucaggagg ccaugaaaaa gauucaacgc     1620

gauuacggca aggaagggua caucucuaga ggagacauug ugcggcucga cgcucuggag     1680

cugcagggcu gcgccugucu uuauugcggg accacgaucg gcuaccacac augucagcuc     1740

gaucauaucg ucccccaggc cggaccuggc uccaacaaua gacgcgggaa ccugguggcu     1800

guuugcgaac gguguaauag aagcaaaucu aacacccccu ucgcggugug ggcccaaaag     1860

ugcggaaucc cucacgucgg cgugaaagag gcuaucgggc gcgugcgggg auggagaaag     1920

cagaccccca acacauccag cgaggaccug acccgccuca aaaaggaagu uauugcccgg     1980

cugagacgca cacaggagga uccggaaauc gacgagcggu ccauggaauc ugucgccugg     2040

auggcuaaug agcuucacca uagaauugcc gcggcuuauc cugagacgac cgugauggug     2100

uaccggggca gcaucacagc cgccgcucgc aaggccgcgg ggauugauuc cagaaucaac     2160

cugaucggcg aaaaaggacg gaaggaccgc auugauagac ggcaccaugc ugucgacgcc     2220

ucugugguug cccucaugga ggcuagcgug gccaaaaccc uggcugaaag auccagccug     2280

cgcggcgagc agcggcuuac agggaaggaa caaaccugga aacaguauac gggaucuacc     2340

gucggcgcca gagagcacuu ugagaugugg cgcgggcaua ugcuccaccu gacagaacug     2400

uucaaugagc ggcucgcgga agauaaggug uacguuaccc agaacaucag acugcgccuu     2460

uccgacggaa augcccacac agugaacccc agcaagcugg ucucucaucg gcucggcgau     2520

gggcugaccg ugcagcaaau ugacagagcu uguacgcccg cccuguggug cgcucuuaca     2580

cgcgagaaag auuuugacga gaagaauggc cucccugcca gagaagaccg ggccauccgc     2640

guucacggac augagauuaa auccagcgau uauauccagg uguucucuaa gagaaagaag     2700

accgacagcg aucgggacga aacaccguuc ggcgcuaucg cgguccgcgg gggauuugug     2760

gagauuggcc ccuccaucca ccaugccaga auuuaccggg uggaagggaa gaaaccuguc     2820

uacgcuaugc ugagaguuuu cacccacgau cugcucagcc agcgccacgg agaccuguuu     2880

ucugccguga uucccccuca guccauuagc augcggugcg ccgagcccaa acuuagaaaa     2940

gcuaucacca caggcaacgc cacguaucug gggugggugg ucgugggcga ugagcucgaa     3000

aucaauguug acuccuucac caaguacgcg auuggacgcu uucuggagga uuucccuaac     3060

acaacccggu ggagaaucug uggcuaugac acaaauucua aacugacccu uaagcccauu     3120

gugcucgcug ccgaagggcu ggagaacccg agcuccgccg ucaaugaaau cguggagcug     3180

aaaggauggc ggguugcuau uaacgugcuc acgaaggucc auccuacagu gguucgcaga     3240

gaugcccugg gccggccccg cuacucuagc agauccaauc uuccuaccuc uuggaccauc     3300

gaguga                                                                3306


<210>  3705
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3705
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

acgaccgaua auuauaaagu uccuucuaag aaaaugaagg ugcucggcaa cacaagcaaa      120

aaguacauca agaaaaaucu gcuuggggug cuccuguucg acuccggaau uaccgcugag      180

ggcagacggc ugaagcgcac agccagacgg cgcuauacca gacggcgcaa cagaauccuu      240

uaccugcagg aaauuuucuc uacagagaug gcgacccucg augacgcuuu cuuucaacgg      300

cuggaugaca gcuuccuggu cccggaugac aaacgcgauu ccaaguaucc caucuuuggg      360

aaucucgugg aagagaaagc cuaccacgac gaauuuccua cgaucuauca ucugagaaag      420

uaccuugccg auucuaccaa gaaagcugac cugcggcucg uuuaccuggc ccuugcucac      480

augauuaagu aucgcggaca uuuucugauc gagggcgagu ucaacagcaa aaauaacgau      540

auucagaaga auuuucagga cuuccucgac acauacaacg ccaucuuuga auccgaucug      600

agccuggaaa auucuaaaca gcucgaagag auugugaagg acaaaaucuc caagcuggag      660

aagaaagauc ggauucuuaa gcuguucccc ggcgagaaaa acagcgggau cuuuucugag      720

uuccucaagc ugaucgucgg aaaucaagcg gacuucagaa aaugcuuuaa ccuugaugaa      780

aaggccuccc ugcacuucag caaggagucu uaugacgaag aucucgagac ccugcugggc      840

uacauugggg acgauuauuc cgacguguuu cucaaagcua agaaacugua cgaugccauc      900

cuucugagcg gauuccucac aguuaccgac aaugagacgg aagcuccucu guccucugcc      960

augauuaagc gcuauaacga gcauaaagaa gaccuugcgc ugcucaagga guacaucaga     1020

aacauuagcc ugaagacaua caaugaagug uuuaaagaug acaccaagaa cggcuaugcc     1080

ggguacaucg auggaaaaac aaaucaggag gacuucuaug ucuaccugaa gaaacuccug     1140

gcugaguuug aaggcgccga uuauuuccuu gagaagaucg accgggaaga uuuucugaga     1200

aagcagcgca ccuucgacaa cggcuccauu cccuaccaga uccaccucca agagaugcgg     1260

gcuauucugg auaaacaggc caaguuuuau ccuuuccugg ccaaaaauaa ggaaagaauc     1320

gagaaaauuc uuaccuuucg caucccguac uacguggggc cccucgcucg gggaaacagc     1380

gacuucgcgu ggucuaucag aaagcgcaau gagaaaauua caccuuggaa cuucgaagau     1440

gugaucgaca aggaguccag cgccgaggcu uuuauuaauc ggaugacguc uuucgaucug     1500

uaucugcccg aagagaaggu ccucccuaaa cacagccugc uuuacgaaac cuuuaacguu     1560

uauaaugagc ugacaaaggu gagauucauc gccgaaucca ugcgcgacua ccaguuucuc     1620

gacucuaaac agaagaaaga uauugugcgg cuguauuuca aggacaagag aaaagucacc     1680

gauaaggaca ucauugagua ccugcaugcc aucuauggcu acgaugggau cgaacuuaaa     1740

ggaauugaga agcaauuuaa cagcucccuc agcacauacc acgaccugcu gaauaucauu     1800

aacgauaaag aguuccucga cgauucuucc aaugaagcua ucauugagga aaucauccau     1860

acccugacga uuuuugagga ccgcgaaaug aucaagcagc ggcuuagcaa guucgagaac     1920

auuuuugaua aaucugugcu gaagaaacuc uccagacggc acuauacagg cugggggaag     1980

cugagcgcca aacugaucaa cgggauucgc gacgagaagu ccggcaauac cauccuugau     2040

uaccucaucg acgacggcau uucuaacaga aauuucaugc agcugaucca cgaugacgcg     2100

cugagcuuua agaaaaagau ucaaaaagcu cagaucauug gggaugaaga caagggaaac     2160

aucaaagagg uugugaaguc ccuccccggc ucuccggcca ucaaaaaggg gauucugcag     2220

agcaucaaga uugucgauga acuugugaaa guuaugggag gccggaagcc ugaguccauc     2280

guggucgaaa uggccagaga gaaucaguau accaaccaag ggaaaagcaa uucucagcag     2340

cgccugaagc ggcucgagaa aucccugaag gaacugggca gcaagauucu uaaagagaac     2400

auccccgcua agcucucuaa aauugacaau aacgcccugc agaaugauag acuguaccuc     2460

uauuaccugc aaaacggaaa ggacauguau acaggcgaug accuugauau cgaccgccug     2520

uccaauuacg acaucgauca uauuaucccu caggcuuucc ucaaagacaa cagcauugau     2580

aauaaggugc uggucucuag cgccuccaac agaggaaaga gcgacgaugu gcccucucug     2640

gaaguuguga aaaagcggaa aaccuucugg uaccagcuuc ugaaguccaa acucaucagc     2700

cagcgcaagu uugacaaccu gacaaaggcg gagagaggag gccucucccc cgaggauaaa     2760

gccggguuca uucaacggca gcuggucgaa acgcgccaga ucaccaagca cguggcuaga     2820

cuucuggacg agaaauuuaa uaacaagaaa gaugaaaaua accgggccgu ucgcacagug     2880

aagaucauua cccucaaguc uacacugguc agccaguuca gaaaagacuu ugagcuguau     2940

aaggugcggg aaaucaauga uuuccaucac gcucaugacg ccuaccuuaa cgcggugguu     3000

gccuccgcuc ugcucaaaaa guauccuaaa cuggagccgg aguuugucua cggagauuau     3060

cccaaguaca auucuuuccg cgaaagaaaa agcgccaccg agaaggugua uuuuuacucc     3120

aacauuauga auaucuucaa gaaaucuauu agccucgcug acggccgggu gaucgaaaga     3180

ccucugaucg aggucaacga agagacgggg gaguccgugu ggaauaagga aagcgaccuu     3240

gccaccguuc gccgggugcu gucuuacccc caagucaacg ugguuaaaaa gguggaggaa     3300

cagaaucacg gccucgauag aggaaaaccu aagggccugu uuaacgccaa ucuguccagc     3360

aagcccaaac cgaacucuaa cgagaaucuu gucggggcua aggaauaucu ggacccuaaa     3420

aaguacggag gcuaugcggg gauuuccaac agcuucacag ugcucguuaa aggaaccauc     3480

gagaagggcg ccaagaaaaa gauuacaaau gugcuggagu uccaggggau cucuauucuc     3540

gaucgcauca acuaccggaa agacaagcug aauuuucuuc uggaaaaagg cuauaaggau     3600

auugagcuga ucaucgaacu ccccaaguac ucccuguucg agcuuagcga cggauccaga     3660

cgcaugcugg cuagcauucu cucuaccaac aauaaacggg gcgagaucca caaggggaac     3720

caaauuuuuc ugagccagaa auucgucaag cuccuguauc augccaaaag aaucuccaau     3780

acgauuaacg aaaaucaccg caaguacgug gagaaccaua aaaaggaauu ugaggaacuu     3840

uucuacuaua uccuggaguu uaaugagaac uacgugggag ccaagaaaaa uggcaagcug     3900

cucaacucug cuuuccagag cuggcagaau cacuccaucg augaacugug uucuagcuuu     3960

auugggccua caggauccga gcggaaaggc cuuuucgaac ugaccucuag agggagcgcc     4020

gcggacuuug aguuccucgg cgucaagauc ccccgguauc gcgauuacac accuuccagc     4080

cugcucaaag acgcuacccu gauucaccaa ucuguuaccg gacuuuauga aacaagaauc     4140

gaucuggcca agcugggcga gggguga                                         4167


<210>  3706
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3706
auggccgcuu ucaagcccaa cagcaucaau uacauucugg gccucgacau cgggauugcc       60

uccgugggau gggcgauggu cgagaucgau gaagaggaaa acccuauuag acugaucgac      120

cuuggcguuc ggguguuuga gcgcgcugaa gugccgaaaa ccggggauuc ucuggccaug      180

gccagacggc ucgcucgcag cgucagacgg cugacacgca gacgggccca ccgccuucug      240

agaacgcggc gccuccugaa gagagaggga gugcugcagg cugccaauuu cgacgagaac      300

ggccucauca aaucccugcc caauaccccu uggcaacuuc gggcggccgc ucuggaucgc      360

aagcucacac cccuggaaug gucugccguu cuucugcauc ucauuaaaca cagaggguau      420

cugagccagc ggaagaacga gggagaaacc gcugacaagg agcugggcgc ccuccugaaa      480

ggcguggcgg ggaaugccca ugcccuucag acaggagauu uuagaacccc ugcugagcug      540

gcucucaaca aguucgaaaa agaguccggc cacauccgca aucagcgguc ugacuacagc      600

cauacauuuu ccagaaagga ucugcaagcc gaacuuauuc uucucuucga gaaacagaag      660

gaauuuggga acccccacgu cagcggaggc cugaaggagg ggaucgagac ccugcucaug      720

acgcagcgcc cugcccuguc uggagacgcu gugcagaaaa ugcuuggcca cugcaccuuc      780

gaaccggcgg agcccaaggc cgcuaaaaau acauauaccg ccgaacgguu uauuuggcug      840

acaaagcuca acaaucugag aauccuugag caaggcuccg aacgcccucu gaccgauacg      900

gagcgggcca cacucaugga cgagcccuac agaaaaagca agcugaccua ugcucaggcc      960

cgcaaacugc ucgggcugga agauacagcg uucuuuaagg gacuucggua cggcaaggac     1020

aacgcugagg ccucuacccu gauggaaaug aaagccuauc augcuauuuc cagagcccuc     1080

gagaaggaag ggcugaaaga caagaaaagc ccucugaauc uuucucccga gcuccaggau     1140

gagaucggaa ccgcuuucuc ccuguuuaag acagacgaag auaucacggg ccgccugaag     1200

gaccggauuc agccggagau ccucgaagcc cugcuuaaac acauuagcuu cgauaaguuc     1260

guucaaaucu cccugaaagc gcucagacgg auugugccuc ugauggagca ggggaagcgc     1320

uacgacgagg ccugugcuga aaucuacgga gaucauuaug gcaaaaagaa caccgaggaa     1380

aagaucuacc ugcccccuau ucccgccgac gagaucagaa accccgucgu gcuucgggcu     1440

cucucucagg ccagaaaagu gauuaauggc gucguucgcc gguaugggag cccugcgaga     1500

auccacauug aaacagcccg cgagguggga aaguccuuua aagauagaaa ggagaucgaa     1560

aaacggcaag aggaaaaccg caaggacaga gagaaggcug ccgcuaaauu ccgggaauac     1620

uuuccgaauu ucgugggcga gcccaagagc aaagauaucc ugaagcugcg ccucuaugag     1680

cagcagcacg ggaaaugccu guacucugga aaggaaauua accuuggcag acugaaugag     1740

aaaggguaug ucgaaaucga ccaugcccuc ccuuuuuccc ggaccuggga cgauagcuuc     1800

aacaauaagg ugcugguucu gggcucugag aaccagaaua agggaaacca aacacccuac     1860

gaauacuuua auggcaaaga caacagccgc gaguggcagg aguucaaggc cagaguggaa     1920

accucccggu uuccucgcuc uaaaaagcag cggauucuuc uccagaaauu cgaugaggac     1980

ggguuuaagg aaagaaaucu gaacgauacg agauauguca aucgcuuccu gugucaauuu     2040

guggcugacc ggaugagacu cacaggaaag ggcaaaaagc gcguuuucgc gagcaacggg     2100

cagaucacca accugcuucg gggauucugg ggccugagaa aagugcgcgc cgagaaugau     2160

cggcaccaug cucucgacgc cgucgugguc gccugcucca ccguggcuau gcagcagaag     2220

auuacaagau uuguucgcua caaagagaug aacgccuucg augggaagac caucgacaag     2280

gaaacaggcg aggugcugca ccagaaaacg cauuuucccc agccguggga guucuuugcg     2340

caggaaguca ugauucgggu guucggaaag ccugauggca aacccgaauu ugaggaggcu     2400

gacacccugg aaaagcugag aacacuccug gccgagaaac ugagcucucg gccugaagcc     2460

guucacgagu augugacacc ccucuucguc ucccgcgcuc cuaauagaaa gaugagcggg     2520

cagggacaca uggaaaccgu gaagucugcc aaacggcugg augagggcgu guccguucuu     2580

cgcguccccc ugacccaacu caagcugaaa gaccuggaga agauggugaa cagagaacgg     2640

gagccgaaac uuuacgaagc gcugaaggcu agacucgagg cccauaaaga cgauccugcc     2700

aaggcuuuug ccgaacccuu cuauaaguac gacaaagcug ggaaucgcac gcaacaggug     2760

aaggccgucc ggguggagca aguucagaaa accggagugu gggucagaaa ccacaauggc     2820

aucgcggaua acgccacaau ggugcgcguu gacguguuug agaaggggga uaaauauuac     2880

cuggucccca ucuacagcug gcaaguggcu aagggcauuc ucccugaccg ggccguugug     2940

cagggaaagg augaagagga cuggcagcug aucgaugacu ccuucaauuu uaaauucucu     3000

cuucauccga acgaucuggu cgaagugauu accaagaaag cuagaauguu cggcuauuuu     3060

gccagcuguc accgcgggac aggaaauauc aacauucgga uccacgaccu cgaucauaag     3120

aucggcaaaa augggauucu ggagggaauc ggcgugaaga ccgcccuguc cuuccagaag     3180

uaccagauug acgagcuugg gaaagaaauc agacccugcc gccugaagaa aagaccuccc     3240

guccgguga                                                             3249


<210>  3707
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3707
augaagaaac ccuacaccau cggccuggac auugggacaa acagcguggg augggccguc       60

cucacggauc aguaugaccu gguuaagaga aaaaugaaga ucgcuggcga uuccgagaaa      120

aagcaaauua agaaaaauuu cuggggagug cggcuuuuug acgaagggca gaccgccgcg      180

gaucgcagaa uggcucggac agcccgcaga cggaucgagc gcagacggaa ccgcauuucu      240

uaccugcagg gcaucuucgc cgaagagaug agcaagaccg acgcuaauuu cuucugcaga      300

cucuccgauu cuuuuuaugu ggacaacgaa aaacggaaua gccgccaccc uuucuuugcc      360

acaaucgagg aggaagucga guaccauaag aacuauccga ccauuuacca ccugagagag      420

gaacuuguua auuccucuga gaaagcugau cugcggcucg uguaucuggc ccuggcgcau      480

aucauuaagu accgcgggaa cuuccucauc gaaggagccc uggacacaca gaauaccagc      540

guggauggca uuuacaagca auuuauccag acguauaacc aggucuucgc uuccggcauu      600

gaggacggga gccuuaaaaa gcuggaagau aauaaagacg uggccaagau ccucguugag      660

aaagugacca gaaaggaaaa acuggagcgg auccuuaagc uguaccccgg agagaagucu      720

gcuggcaugu uugcccaguu cauuucccuc aucgucggga gcaaaggaaa cuuucaaaag      780

ccuuucgacc ugauugaaaa aucugauauc gagugugcga aggacuccua ugaagaggau      840

cuggaaagcc uccuggcccu uauuggcgac gaguacgcug agcuguucgu ggccgcuaaa      900

aaugccuauu cugccguggu ccucuccagc aucaucacag uugcugaaac cgagacaaac      960

gcgaagcugu ccgccucuau gauugaaaga uuugauaccc acgaggaaga ccuuggggag     1020

cugaaggcuu ucaucaaacu ccaucugccc aagcacuacg aggaaauuuu uagcaauacg     1080

gagaaacacg gauaugccgg cuacaucgau ggcaagacaa aacaggccga cuucuacaag     1140

uauaugaaga ugacccugga aaacauugag ggggcugauu acuuuaucgc caaaaucgag     1200

aaggaaaacu uccuccgcaa acagcggaca uuugacaaug gagcgauucc ucaucagcug     1260

caccuugagg aacuggaggc uauccuccau caacaggcca aguauuaccc cuuccugaaa     1320

gaaaacuaug auaagauuaa gucccuugug accuuuagaa ucccuuacuu cgugggcccg     1380

cuggccaaug ggcagagcga guuugcuugg cucacccgca aagccgacgg agagauucgg     1440

cccuggaaca ucgaagagaa ggucgacuuc ggcaaaucug cuguggauuu uauugaaaag     1500

augacaaaua aagacacgua ucugccuaag gagaacguuc ugcccaaaca cucccucugc     1560

uaccaaaagu accuggugua uaaugaacuu accaagguca gauacaucaa cgaucagggg     1620

aaaacaagcu auuucucugg acaggagaag gagcagaucu ucaaugaccu guuuaaacaa     1680

aagcgcaaag ugaagaagaa agaucucgaa cuguuccugc ggaacaugag ccacguugag     1740

uccccuacca uugaaggccu ugaggacucu uuuaauagcu ccuacagcac auaucaugau     1800

cuccugaagg ugggcaucaa acaggaaauu cuggacaacc ccgucaauac cgagaugcuc     1860

gagaacaucg ugaagauucu gacggucuuc gaagauaaaa gaaugaucaa ggagcagcuu     1920

cagcaauuuu cugacgugcu ggaugggguu gugcucaaga aacuggaacg ccggcacuac     1980

acaggauggg gcagacuguc cgccaagcuc cucaugggga uccgcgacaa acagagccau     2040

cugaccauuc uggauuaucu caugaaugac gacggacuga accggaaccu uaugcagcug     2100

aucaaugauu cuaaccucuc cuucaagagc auuaucgaga aagagcaggu caccacagcg     2160

gacaaggaua uucaauccau cguggccgac cuggcuggcu cuccggccau caagaaaggg     2220

auucugcaga gccuuaagau cguugaugaa cucguguccg ucaugggcua cccuccccag     2280

accauugugg uggagauggc uagagaaaau cagacaacgg gaaaaggcaa gaacaauucu     2340

cggccucgcu acaaaagccu ggagaaggcc aucaaagaau uuggguccca aauucugaag     2400

gagcacccca ccgacaacca ggagcucaga aauaaccggc uguaucuuua cuaucugcag     2460

aauggaaagg auauguacac aggccaagac cucgauaucc auaaccugag caauuaugac     2520

aucgaucaca uuguucccca gucuuucauc accgacaacu ccauugauaa ucugguccuu     2580

acaagcucug cggggaacag agaaaaagga gacgacgugc cuccgcucga gaucgugcgc     2640

aagcggaaag ucuuuuggga aaagcuguac cagggcaauc ugauguccaa aagaaaguuc     2700

gauuaucuca ccaaggccga gcgcgggggc cugacggaag cugacaaagc cagauuuauu     2760

caccggcagc uuguggagac ccgccaaauc acaaagaacg uugcuaacau ucugcaucag     2820

agauucaauu acgagaaaga ugaccacgga aacaccauga aacaggugcg gaucgucaca     2880

cucaagagcg cccugguguc ucaguuucgc aagcaauucc agcuguacaa gguuagagau     2940

gugaaugacu aucaucacgc ccacgaugcu uaccuuaacg gcgucguggc gaauacccug     3000

cucaaaguuu auccccagcu ggaaccugag uucguguacg gggacuauca ucaguuugau     3060

ugguucaagg ccaacaaagc uacggccaag aaacaauuuu acacaaauau caugcuuuuc     3120

uuugcccaga aggaccggau uaucgaugaa aacggagaga uucuguggga caagaaauau     3180

cuugacaccg ucaagaaagu gaugagcuac cgccagauga auaucgugaa gaaaacagaa     3240

auucaaaagg gcgaguucuc caaggcuacc aucaaaccca aggggaacag cucuaaacug     3300

aucccuagaa agaccaauug ggaucccaug aaauacggag gccucgacuc cccgaacaug     3360

gccuaugcgg ucguuauuga guacgcuaag gggaaaaaua agcugguguu ugaaaagaaa     3420

aucauucggg ugacaaucau ggagcgcaag gccuucgaaa aagaugagaa ggccuuucug     3480

gaggaacagg gcuauagaca gccuaaaguc cuugcuaagc ugcccaagua cacgcucuau     3540

gagugugaag agggacggag acgcaugcug gccagcgcga acgaagcuca gaaaggcaau     3600

caacaggugc ucccuaacca ccugguuacc cuucugcauc acgccgccaa cugcgaggug     3660

uccgacggga agucucucga uuacauugag agcaaucggg aaauguucgc ugagcugcug     3720

gcccaugucu ccgaauuugc uaaaagauau acacuugccg aggcgaaccu gaauaagauc     3780

aaccagcucu uugaacagaa uaaagaggga gacaucaagg ccauugcuca aucuuucgug     3840

gaucugaugg ccuuuaacgc uaugggcgcc cccgccagcu ucaaguuuuu cgagaccaca     3900

aucgaacgca aacgguacaa uaaccucaag gagcugcuua auuccaccau uaucuaccag     3960

ucuauuacgg ggcuguauga aagcagaaaa cgccuggacg auuga                     4005


<210>  3708
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3708
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

guuacagaug acuauaaggu gccugcuaaa aagaugaaag ugcucggcaa uacggauaag      120

ucucacaucg agaagaaccu gcuuggggcc cuucucuucg acagcggaaa uaccgcggaa      180

gauagacggc ugaaacgcac agcuagacgg cgcuacacca gacggcgcaa ccggauucuu      240

uaucugcagg agaucuuuuc cgaagagaug ggcaaggucg acgauucuuu cuuucaucgg      300

cucgaagaca gcuuccuggu gacagaggau aaacgcgggg agagacaccc gauuuuugga      360

aaucuggaag aggaaguuaa guaccaugag aacuucccca ccaucuauca ccuccggcaa      420

uaccuggccg acaauccuga gaaaguggau cuucgccugg ucuaucucgc ccuggcucau      480

aucauuaagu uuagaggcca cuuccuuauc gaaggcaagu uugacacacg gaacaaugau      540

gugcagcgcc uguuccagga guuucucgcc guuuacgaca acaccuucga aaauuccucu      600

cugcaggagc aaaacgugca ggucgaagag auucugacgg acaaaaucag caaguccgcu      660

aaaaaggaua gagugcucaa acuguuuccc aaugaaaaga gcaacgggcg guucgccgag      720

uuccuuaaac ugauuguggg aaaucaggcg gacuuuaaga agcacuucga gcucgaagag      780

aaagccccuc ugcaguuuuc uaaggauacc uacgaagagg agcuugaagu ccugcucgcu      840

caaaucggcg acaacuaugc cgagcuguuc cuguccgcua aaaagcucua cgauagcauu      900

cugcuuucug ggauccugac aguuaccgac gugggaacaa aagcgccccu cuccgccagc      960

augauccaga gauauaacga acaucagaug gaucuggccc agcuuaagca auuuauucgc     1020

cagaagcugu cugacaaaua caaugaggug uucuccgaug ucagcaagga cggcuaugcu     1080

ggguacaucg auggaaaaac caaccaggaa gccuuuuaua aguaccucaa aggccugcug     1140

aauaagauug agggcuccgg guacuuccuc gacaagaucg agcgggaaga cuuucugaga     1200

aaacaacgca cguucgauaa cggaucuauu ccucaccaga uccaucuuca ggagaugcgg     1260

gcuaucauua gacgccaggc cgaauuuuau ccguuccugg ccgacaauca agaucggauc     1320

gagaagcucc ugacauuucg cauucccuac uaugugggcc cucuggcuag agggaaaagc     1380

gacuucgcgu ggcuuuccag aaagagcgcc gauaaaauca cccccuggaa cuucgacgag     1440

auuguugaua aggaaucuuc cgcugaggcc uuuaucaaua gaaugacaaa cuacgaccuc     1500

uaucugccua aucagaaggu gcugcccaaa cacagccucc uguacgaaaa guucaccguc     1560

uauaacgagc uuaccaaagu gaaguacaaa acagaacagg gaaagacggc cuuuuucgau     1620

gcuaauauga aacaggagau cuuugacggc guuuucaagg uguaccgcaa ggucaccaaa     1680

gauaagcuga uggacuuucu cgagaaagag uucgaugaau uucggauugu ggaccugaca     1740

gggcuggaca aggaaaacaa agucuucaau gccucuuaug gaaccuacca cgaucuuugc     1800

aagauccucg acaaggauuu ucuggacaac agcaaaaaug agaagauucu ggaagauauc     1860

gugcucacac ugacccuuuu cgaggacaga gagaugauuc ggaaacgccu ggaaaacuau     1920

uccgaucucc ugacgaagga gcaaguuaaa aagcuggaaa gacggcauua cacaggcugg     1980

ggcagacuuu cugcggagcu cauccacggg auucgcaaca aggaaagccg gaaaaccauc     2040

cuggacuauc ugaucgauga cggaaauucc aacagaaauu uuaugcagcu cauuaacgau     2100

gacgcucuga gcuucaagga ggagaucgcc aaagcccagg ugauuggcga aaccgauaau     2160

cuuaaccagg ucgugucuga caucgcuggg uccccggcca uuaagaaagg aauccugcaa     2220

agccucaaga ucguugacga gcuggugaag auuaugggcc aucagccuga aaauaucguc     2280

guggagaugg cucgcgagaa ccaguucaca aaucagggga gacggaacuc ucaacagcgc     2340

cugaaaggcc uuaccgauuc cauuaaggaa uuuggaagcc agauccucaa agagcacccc     2400

guggaaaauu cccaacugca gaacgacaga cuguuccucu acuaucugca gaauggccgg     2460

gauauguaca caggggagga acuugacauu gauuaccugu cucaguauga caucgaucau     2520

aucauuccuc aagccuuuau caaggacaac agcauugaua aucgcguucu cacguccucu     2580

aaagagaaca gaggaaagag cgacgauguc cccuccaaag acguggugcg gaagaugaaa     2640

agcuacuggu cuaaacugcu guccgcgaag cugaucaccc agcgcaaauu cgacaaccug     2700

acaaaggccg agagaggcgg gcucaccgau gacgauaaag cuggauuuau uaagcggcag     2760

cuggucgaaa cacgccagau caccaagcac guggccagaa uccucgacga gcgguucaau     2820

acggaaaccg augagaacaa uaaaaagauu agacaaguua aaaucgugac acuuaagagc     2880

aaccuggucu cuaauuuucg caaagaguuc gaacuguaua aggugcggga gauuaacgac     2940

uaccaccaug cucacgaugc cuaucucaau gcgguuaucg gcaaggcccu gcugggggug     3000

uacccccagc uugaaccuga guuugucuau ggcgacuacc cgcauuucca cggacacaaa     3060

gaaaacaagg cuaccgccaa aaaguuuuuc uacuccaaua uuaugaacuu cuuuaaaaag     3120

gaugacguga gaacagauaa gaauggcgag aucauuugga aaaaggacga acauaucagc     3180

aacaucaaaa agguucuguc uuauccccag gugaauauug ucaaaaaggu ggaggagcag     3240

accgggggau ucagcaagga auccauccuc ccuaaaggca acagcgauaa gcugauuccc     3300

cgcaaaacga agaaauuuua cugggacaca aagaaauaug ggggauucga cucuccuauc     3360

guggcuuacu ccauucucgu caucgccgau aucgagaagg gcaagagcaa aaagcugaaa     3420

accguuaagg cccuuguggg ggugacaauu auggaaaaga ugaccuuuga gcgggacccc     3480

gucgcuuucc uggagagaaa gggcuaucgc aaugugcaag aagagaacau cauuaagcuc     3540

ccgaaauacu cccuguuuaa gcuggaaaac ggacggaaaa gacuucuggc gucugcccgc     3600

gagcuccaga agggcaauga aaucguucug ccuaaccacc ucgggacccu gcuuuaucau     3660

gcuaaaaaua uucacaaggu ggaugagccc aagcaucugg acuacgucga uaaacacaag     3720

gacgaguuca aagaacugcu cgaugugguu agcaacuuuu ccaagaaaua cacacuggcc     3780

gagggaaauc uugaaaagau caaggagcug uaugcccaga acaauggcga agaccucaaa     3840

gagcuggcuu cuagcuucau caaccuccug acguuuaccg ccauuggggc gccugcuaca     3900

uucaaguuuu ucgauaaaaa uaucgaccgg aagagauaca ccuccacaac cgagauucuu     3960

aacgccacgc ugauccacca aucuauuaca ggacuguaug aaacccggau cgaucucaau     4020

aaacugggcg gggacuga                                                   4038


<210>  3709
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3709
augcagacca caaaccugag cuacauccuc ggccuggacc uugggauugc cuccguggga       60

ugggcugucg uugagaucaa ugaaaacgag gaucccauug gccugaucga cgugggggug      120

agaauuuucg aacgggccga ggucccuaag acgggagaau cucucgcgcu gagccgcaga      180

cuugcucggu ccacccgcag acugauccgg cgcagagccc accggcuccu gcuggccaaa      240

cgcuuucuca agagagaggg cauccugucu acaauugauc uugagaaagg gcugccgaau      300

caggcuuggg aacuccgggu ggccggacug gagcgcagac uuagcgcuau cgaauggggc      360

gccguucugc uccaucugau uaagcaccgg ggcuaucugu ccaaacgcaa gaacgagucu      420

cagaccaaua acaaggagcu cggggcgcug cuuagcggag uggcccagaa ucaucagcug      480

cuccaauccg acgauuacag aacacccgcu gaacuggccc uuaaaaaguu cgcuaaagag      540

gaaggccaca uccggaacca gagaggggcc uauacccaua cauuuaaucg ccuggaccuc      600

cuggcggagc ugaaccuccu guucgcccag cagcaccaau uuggaaaucc ucacugcaag      660

gaacauauuc agcaguacau gaccgagcuu cugauguggc agaaacccgc ucucagcggc      720

gaggccaucc ugaagaugcu ugggaagugu acgcacgaaa agaacgaguu caaagcugcc      780

aaacauaccu auucugccga acgguuuguc uggcugacaa agcucaauaa ccugcgcauu      840

cuggaggaug gagcugaaag agcgcucaau gaggaggaac ggcaacugcu uaucaaccac      900

ccuuacgaga aauccaagcu gaccuaugcc caggugagaa aacuccuggg ccugagcgaa      960

caggcuaucu ucaagcaccu ucgcuacucu aaggagaacg ccgaauccgc cacauuuaug     1020

gagcucaagg cuuggcaugc cauucggaaa gcgcuggaga aucaaggccu gaaagacacc     1080

uggcaggauc ucgcuaagaa acccgaccug cuugaugaaa ucgggacggc cuucagccug     1140

uacaagacag acgaggauau ucagcaguau cucaccaaca agguuccuaa uucugugauc     1200

aacgcccugc uggucucccu uaauuuugac aaauucauug aacucagccu gaagucccug     1260

agaaaaaucc ucccgcugau ggagcaagga aagcgcuacg accaggcuug ccgggagauc     1320

uauggccacc auuacgggga agccaaccag aaaacaucuc agcuucugcc cgcuauuccu     1380

gcccaagaga ucagaaaucc cguggugcuc cgcacccuga gccaggcgcg gaaggucauu     1440

aacgccauca uuagacagua uggauccccu gcucggguuc acaucgaaac cggccgcgag     1500

cuggggaagu ccuuuaaaga aagacgggag auccagaagc aacaggagga uaauagaaca     1560

aaacgcgaau cugccgugca gaaguucaaa gagcuuuucu ccgacuuuag cucugaaccc     1620

aagagcaagg auauucucaa auuccggcug uacgagcagc aacauggaaa gugucuguau     1680

uccggcaaag aaaucaacau ucacagacuc aaugagaagg gcuacgugga gaucgaccac     1740

gcucugccgu uuucucgcac gugggaugac agcuucaaca auaaaguccu ugugcuggcc     1800

uccgaaaacc agaauaaggg gaaccagacc ccuuacgagu ggcuccaagg aaaaauuaac     1860

agcgaaagau ggaagaauuu uguugcgcug gugcugggcu cucagugcuc cgccgcuaag     1920

aaacagcggc uucucacaca ggucaucgau gacaacaagu ucauugaucg caaucugaac     1980

gacaccagau auaucgcccg guuucugagc aauuacaucc aagagaaccu ccugcuugug     2040

gggaaaaaua agaaaaacgu uuucacaccc aauggacaga uuaccgcucu gcuccgcucu     2100

agauggggcc ugaucaaggc ccgggaaaac aauaaccgcc aucacgcccu ggaugcuauu     2160

guggucgcgu gugccacgcc uuccaugcag cagaagauca caagauuuau ucgguucaaa     2220

gaggugcauc ccuauaagau cgagaaucgc uacgaaaugg ucgaccaaga gagcggggaa     2280

aucauuagcc cccacuuucc ugagccgugg gcuuauuuca gacaggaggu gaacauccgg     2340

guuuuugaca aucaccccga uaccgugcuu aaagaaaugc ugccugacag accccaggcc     2400

aaccaucagu ucguccaacc ucucuucgug ucucgcgccc ccacccggaa gaugagcggc     2460

cagggacaca uggagacaau uaaauccgcu aagagacugg ccgaaggcau cucuguucuc     2520

cgcauuccgc ugacccagcu uaagccuaac cugcucgaga auauggugaa caaagaacgg     2580

gagcccgcgc uguacgcugg gcugaaggcc agacuugccg aguuuaauca agauccugcu     2640

aaagccuucg cgacacccuu uuauaagcag ggaggccagc aggucaaagc uauccgcgug     2700

gaacaagugc aaaagagcgg gguucugguc cgggagaaca auggaguggc cgacaacgcc     2760

uccaucguga gaacggaugu cuucauuaag aauaacaaau uuuuccucgu gccuaucuac     2820

accuggcagg uugcuaaggg cauucugccc aauaaagcca ucguggcuca uaagaacgaa     2880

gacgaguggg aagagaugga ugagggggcc aaauuuaagu ucagccucuu uccgaaugac     2940

cuggucgaac uuaaaacaaa gaaggaguac uucuuuggcu auuacauugg acuggaucgc     3000

gcgaccggca acaucucucu caaagaacac gacggggaga ucuccaaggg aaaagauggc     3060

guguaucggg uuggggugaa gcuggcccug agcuucgaaa aauaccaggu cgacgagcuu     3120

ggaaagaaua gacaaauuug ccggccucag cagcgccagc ccgugagaug a              3171


<210>  3710
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3710
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagacgaga acccgauuug ccugaucgau      120

cuuggcguua gaguguuuga acgggcugag gugcccaaaa ccggggacuc ccuggccaug      180

gcccgcagac ucgcucgguc uguccgcaga cugacacggc gcagagccca ccggcuucug      240

cgcgcuagac ggcuccugaa gcgcgaagga gugcugcagg ccgcggauuu cgacgagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuua gagccgcugc ccuggaucgg      360

aagcucaccc cucuggagug guccgcuguu cuucugcauc ucauuaaaca ccgcggguau      420

cugucucaga gaaagaauga aggagagaca gccgacaagg aacugggcgc gcuccugaaa      480

ggcguggccg auaacgcuca ugcccuucag accggggacu uucggacacc cgcugagcug      540

gcccucaaua aguucgagaa agaaagcgga cacaucagaa accagcgcgg cgauuacucc      600

cauaccuuuu cucggaagga ccugcaagcc gagcuuauuc uccuguucga aaaacagaag      660

gaguuuggga auccucacgu cagcggaggc cugaaggaag ggaucgagac acugcucaug      720

acccagagac cggcucuguc cggagaugcg gugcagaaaa ugcuuggcca cuguacguuc      780

gagcccgccg aaccuaaggc ugccaaaaac accuauacag ccgagcgcuu uauuuggcug      840

accaagcuca auaaccugcg gauccuugaa caaggcagcg agagaccccu gacagacacc      900

gaacgcgcua cgcucaugga cgagccuuac cggaaaucua agcugacaua ugcccaggcg      960

agaaaacugc ucgggcugga ggauaccgcu uucuuuaagg gacuucgcua cggcaaggac     1020

aaugccgaag ccuccacacu gauggagaug aaggcuuauc augccauuag ccgggcucuc     1080

gaaaaagagg ggcugaaaga uaagaaaucu ccccugaacc uuuccccgga acuccaggac     1140

gagaucggaa ccgccuucag ccuguuuaag accgaugagg acaucacagg cagacugaag     1200

gaucgcauuc agccugaaau ccucgaggcg cugcuuaaac acauuucuuu cgacaaguuc     1260

guucaaaucu cccugaaagc ccuccggaga auugugcccc ugauggaaca ggggaagcgg     1320

uacgaugagg cuugcgccga gaucuacgga gaccauuaug gcaaaaagaa uacggaagag     1380

aagaucuacc ugccucccau ucccgcugau gaaauccgca acccugucgu gcuuagagcc     1440

cucagccagg cgcggaaagu gauuaacggc gucguuagac gcuauggguc cccggcccgg     1500

auccacauug agaccgcuag agaaguggga aagucuuuua aagaccgcaa ggagaucgag     1560

aaaagacaag aagagaaucg gaaggaccgc gaaaaggccg cugccaaauu cagagaguac     1620

uuucccaacu ucgugggcga accuaagagc aaagauaucc ugaagcugcg gcucuaugag     1680

cagcagcacg ggaaaugucu guacuccgga aaggagauua aucuuggccg ccugaacgaa     1740

aaaggguaug ucgagaucga ccaugcccuc cccuuuagca gaacauggga ugacucuuuc     1800

aauaacaagg ugcugguucu gggcuccgaa aaucagaaca agggaaauca aaccccuuac     1860

gaguacuuua acggcaaaga uaauagccgg gaauggcagg aguucaaggc ucgcguggag     1920

acaucuagau uuccccggag caaaaagcag cgcauucuuc uccagaaauu cgacgaagau     1980

ggguuuaagg agagaaaccu gaaugacacc cgguauguca acagauuccu gugccaauuu     2040

guggcggauc gcaugcggcu cacgggaaag ggcaaaaaga gaguuuucgc cuccaauggg     2100

cagaucacaa accugcuucg cggauucugg ggccugcgga aagugagagc ugaaaacgac     2160

cgccaccaug cccucgaugc cgucgugguc gcuuguucua ccguggccau gcagcagaag     2220

auuacccggu uuguuagaua caaagagaug aaugcguucg acgggaagac aaucgauaag     2280

gagaccggcg aagugcugca ccagaaaaca cauuuuccgc agccuuggga guucuuugcu     2340

caggaaguca ugauucgcgu guucggaaag cccgacggca aaccugaguu ugaagaggcc     2400

gacacgcccg agaagcugcg gacccuucuc gccgaaaaac ugagcuccag accugaggcu     2460

guucacgaau augugacacc ccuguucguc agccgggccc cgaaccgcaa gaugucuggg     2520

cagggacaca uggagaccgu gaaguccgcg aaaagacucg augaaggcgu gagcguucug     2580

cgggucccuc uuacacaacu gaagcucaaa gaccuggaga agauggugaa ucgcgagaga     2640

gaacccaaac uguacgaggc ucuuaaggcc cggcuggaag cccauaaaga ugaccccgcu     2700

aaggccuuug cugagccuuu cuauaaguac gauaaagccg ggaacagaac ccaacaggug     2760

aaggcggucc gcguggaaca aguucagaaa acgggagugu ggguccggaa ucacaacggc     2820

aucgccgaca augcuaccau ggugagaguu gauguguuug agaaggggga caaauauuac     2880

cucgucccga ucuacucuug gcaaguggcc aagggcauuc ugcccgaucg cgcuguugug     2940

cagggaaagg acgaggaaga uuggcagcuc aucgacgauu ccuucaacuu uaaauucagc     3000

cugcauccua augaccuugu cgaggugauu acaaagaaag cccggauguu cggcuauuuu     3060

gccuccugcc acagagggac cggaaacauc aauauucgca uccacgaccu ggaucauaag     3120

aucggcaaaa acgggauucu cgaaggaauc ggcgugaaga cagcucuguc uuuccagaag     3180

uaccagauug acgagcuggg gaaagagauc cggcccugua gacuuaagaa acgcccuccc     3240

gucagauga                                                             3249


<210>  3711
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3711
augaaguacc acgugggcau cgacgucggg accuucagcg uuggacuggc cgcuauugag       60

guggaugacg ccggcaugcc caucaaaaca cucucccugg ugucucauau ucacgauagc      120

gggcuugacc cugaugaaau caaguccgcg gucacgagac uggcuucuag cggaauugcc      180

cggcgcacca gacggcucua ucgcagaaaa cggcgcagac ugcagcaacu ugacaaguuu      240

auccagcggc agggcuggcc ggugaucgag cuggaagauu acuccgaccc ccucuauccu      300

uggaaaguuc gcgccgagcu ggcugccucu uacauugcug augaaaagga gagaggggag      360

aagcugagcg uggcccuccg gcauaucgcg cgccacagag gauggcggaa ccccuaugcc      420

aaagucucca gccuguaccu uccugacggc cccucugaug cuuucaaggc cauucgcgaa      480

gagaucaaaa gagcuuccgg ccagccugug ccggaaacag ccaccguugg gcaaauggug      540

acacugugcg agcucggaac ccugaaacuu cggggcgaag ggggaguccu gagcgcgaga      600

cuccagcagu cugacuaugc ccgcgaaauu caggagaucu gucggaugca agaaauuggc      660

caggagcugu acagaaaaau caucgaugug guguuugcug ccgaaucccc caaggggagc      720

gcuucuuccc gcgucggaaa ggacccucug cagcccggca aaaaucgggc ccucaaggcc      780

agcgacgcuu uccagagaua ccgcauugcg gcccugaucg gcaaccuucg gguuagagug      840

gauggggaga aacgcauucu guccguggag gaaaagaauc ucgucuuuga ccaucuggug      900

aaccuuacac cuaaaaagga gcccgaaugg guuaccaucg cugagauucu gggaaucgau      960

cggggccaac ucaucgggac ggccaccaug acagacgaug gagaaagagc cggcgcucgc     1020

ccgccuaccc acgacacaaa ucggucuauu gugaacagca gaaucgcucc ccuggucgau     1080

ugguggaaaa ccgcguccgc ucuggagcag cacgccaugg ugaaggcccu cagcaaugcu     1140

gagguugacg auuucgacuc uccugaaggg gccaaggugc aggccuuuuu cgccgaucug     1200

gacgacgaug uccaugcgaa acuugacucc cugcaccucc ccgugggacg ggccgcuuau     1260

agcgaggaua cgcugguccg ccuuacaaga cggaugcugu cugacggcgu ggaucucuac     1320

accgccagac ugcaagaauu uggcauugag cccagcugga caccuccgac accccgcauc     1380

ggggaaccug uuggaaaccc cgcuguggac cggguccuga agaccguguc cagauggcuc     1440

gagucugcca ccaaaacgug gggcgcgccu gagcgcguua uuaucgaaca ugugagagag     1500

ggguucguca ccgaaaagcg ggcccgcgag auggauggag acaugagacg gcgcgcugcc     1560

agaaaugcua aacuguuuca ggagaugcag gaaaaacuua acgugcaggg caagcccagc     1620

cgggccgauc uguggcgcua ucaauccgug cagagacaga auugccagug ugccuacugc     1680

gggagcccga ucacauucuc uaacuccgag auggaccaca uuguuccucg ggcuggccaa     1740

ggaagcacca auacacgcga aaaccucguc gcgguguguc acagaugcaa ucagucuaaa     1800

ggcaacaccc ccuuugccau cugggcuaag aacacgucca uugagggggu gagcgucaaa     1860

gaagccgugg agcggacaag acauuggguu accgauaccg gaaugcgcuc cacagacuuc     1920

aagaaauuca ccaaggccgu ggucgagcgg uuucagagag cuacaaugga ugaagagauc     1980

gacgcccgcu cuauggaaag cguggcgugg auggcuaaug agcugcgguc cagaguugcc     2040

cagcacuucg ccucucaugg cacgaccgug cgcgucuauc gggggagccu gacagcugaa     2100

gccagacgcg cguccggaau uagcggcaaa cuuaaguuuu ucgacggggu gggcaagucu     2160

cggcucgaua gacggcacca ugcuaucgac gccgccguua uugcuuuuac cuccgauuac     2220

guggccgaga cacuggcugu ccgcagcaac cugaaacaau cucaggccca cagacaggag     2280

gcgccucagu ggcgggaguu caccggaaag gacgccgagc accgcgcugc cuggagagug     2340

uggugucaaa agauggaaaa gcucuccgcu cugcuuacgg aggaucugcg ggacgauaga     2400

guggucguua ugagcaaugu gcgccuccgg cugggcaacg ggucugccca uaaggaaacc     2460

aucggaaaac ugagcaaggu gaaacuuucc agccagcucu cugucuccga caucgauaag     2520

gccagcuccg aggcucugug gugcgcgcug acaagagagc ccggcuuuga cccuaaagaa     2580

gggcuccccg ccaauccgga gcgccacauu cgggugaacg gaacccaugu uuaugcuggc     2640

gauaauaucg ggcuguuccc ugugucugcc ggcagcauug cccuuagagg aggcuacgcu     2700

gaacuggggu ccucuuuuca ccacgcccgc gucuacaaga ucacaagcgg aaagaagccc     2760

gcguucgcua ugcuccgggu guauaccauu gaccugcugc ccuacagaaa ccaggaucuc     2820

uuuuccguug agcuuaagcc ucaaacgaug ucuaugcgcc aggccgagaa aaagcugaga     2880

gacgcccugg cuacaggcaa ugccgaauau cucggguggc ugguggucga cgaugagcuu     2940

gugguugaca ccagcaaaau cgcgacagau caggugaagg cugucgaagc cgagcuggga     3000

accauccggc gcuggagagu ggacggcuuc uuuuccccga gcaaacuccg gcugcgcccc     3060

cugcagaugu cuaaggaagg gauuaagaaa gaguccgccc cugagcuuag caagaucauu     3120

gauagacccg gcuggcuccc ugcugugaac aaacuguucu cugacggaaa ugucaccguu     3180

gugcggagag auucccuggg ccgcgugcgg cucgaaagca cagcccaucu gcccgucacg     3240

uggaaggugc aauga                                                      3255


<210>  3712
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3712
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagucgagaa ucccaagaca      120

ggcgaaagcc uggcgcuucc ucgcagacug gcucgguccg cccgcaaaag acucgcccgg      180

cgcaaggcua gacugaacca ccuuaaacau cugauugcca augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gcuaaggccu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcgcugaau gaacuucuga gcaagcaaga uuucgccaga      360

guuauccucc acauugcuaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg ccauucugaa ggcuaucaaa cagaaugaag agaaacuugc caacuaucag      480

agcguggggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugcgca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaaguccu cagcguggcc uuuuauaaga gagcucugaa ggauuucucu      720

caucuuguug gcaacugcuc cuuuuucacc gacgagaaac gggcccccaa gaauagcccu      780

cuggcuuuua uguucguggc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugccc ugcucaacga gguccugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac guauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcucuuggag agcacaaccu gucucaggac gaucucaaug aaauugcgaa agacaucacc     1080

cugauuaagg augagaucaa acugaagaaa gcccucgcua aguaugaccu gaaccaaaau     1140

cagaucgaua gccuuuccaa acuggaguuu aaggaccauc ucaacauuag cuucaaggcc     1200

cugaaacugg ugacaccccu uaugcucgaa ggcaagaaau acgaugaggc cuguaaugaa     1260

cugaaccuga agguugcuau caaugaggac aaaaaggauu uucucccugc cuucaacgag     1320

accuacuaua aggacgaagu gacaaauccc gucgugcugc gcgcgauuaa agaguaccgg     1380

aaggugcuua acgcucugcu caaaaaguau gggaaagucc acaagaucaa uauugaacug     1440

gccagagagg uuggaaagaa ccacucucag cgcgccaaaa ucgaaaagga gcagaacgag     1500

aauuacaaag cuaagaaaga cgccgaacug gagugcgaaa aacuuggccu caagaucaac     1560

uccaaaaaua uucugaagcu gagacucuuc aaagagcaaa aggaauuuug ugcuuauagc     1620

ggcgagaaaa ucaagauuuc ugaucugcag gacgagaaaa ugcuugaaau cgaucauauu     1680

uacccuuaua gccgguccuu cgacgauucu uacaugaaca aggugcuggu guuuaccaag     1740

cagaaucaag agaaacucaa ccagacgccg uucgaagccu uugggaauga cagcgcgaag     1800

uggcagaaaa ucgagguccu ggccaagaac cugcccacaa agaagcagaa gcgcauucuu     1860

gauaaaaauu acaaggacaa agaacaaaag aacuucaaag aucggaaucu caacgacacc     1920

agauauaucg cucgccuggu gcugaauuac acaaaggauu aucucgacuu ucugccucuu     1980

uccgaugacg agaacaccaa gcugaaugac acccagaaag gaagcaaggu ucacguggag     2040

gccaaaucug gcaugcucac auccgcucug cggcauacgu ggggguucag cgccaaggau     2100

agaaacaacc aucugcacca cgcgaucgac gccgucauua ucgcuuacgc caauaacucu     2160

auugugaagg cuuuuuccga uuucaagaag gaacaggaga gcaauuccgc cgaacuuuau     2220

gccaaaaaga ucucugagcu cgacuacaaa aacaagcgca aauuuuucga gcccuuuagc     2280

ggauuccggc agaagguucu ggauaagauu gacgaaaucu ucguguccaa accugagaga     2340

aagaaacccu cuggcgcucu gcacgaagag accuuucgga aggaagagga guucuaucaa     2400

agcuacgggg gaaaagaagg cguccucaag gcgcuggagc uuggcaaaau ccgcaaggug     2460

aaugggaaga uugucaaaaa cggagauaug uuuagagugg acaucuucaa gcauaaaaag     2520

acaaauaaau uuuacgccgu uccgauuuau accauggauu ucgcucugaa ggugcucccu     2580

aacaaggccg ucgcccgguc caaaaagggc gaaaucaaag acuggauucu gauggaugag     2640

aauuacgaau uuugcuucag ccuguauaag gacucucuua uccugaucca gacaaaagau     2700

augcaggagc ccgaguuugu guacuauaac gcuuucaccu ccagcacggu uucucucauu     2760

guguccaagc acgacaauaa guuugaaaca cugagcaaaa accagaagau ccucuucaaa     2820

aaugccaacg agaaggaagu cauugcgaaa ucuaucggga uucaaaaucu gaagguguuc     2880

gagaaguaca ucgugagcgc ucuuggagaa guuaccaaag ccgaguuuag acagcgcgag     2940

gacuucaaga aauga                                                      2955


<210>  3713
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3713
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggugcggcg cacaaacaga caaggccggc gccucgcgag acggaagaaa      180

caucgcagag uccggcugaa ucgccuuuuu gaggaaagcg ggcugaucac ggacuucacc      240

aagaucucca uuaaccucaa uccuuaccag cugagaguga aaggacugac agaugagcuc      300

ucuaacgagg agcuguuuau cgcucuuaag aauaugguua agcaccgggg cauuagcuau      360

cuggacgaug ccuccgacga uggcaacucu agcguggggg acuacgccca gaucgucaaa      420

gaaaauucca agcagcucga aaccaaaaca ccgggacaaa uucagcugga gcgcuaucag      480

accuacggcc agcuuagagg ggauuucaca guggaaaagg acggaaagaa gcaucggcug      540

aucaacguuu uucccaccag cgcuuaucgc ucugaggccc uccggauucu gcaaacgcag      600

caggaguuca auccucagau caccgaugaa uuuaucaaca gauaccugga gauucucaca      660

ggcaagagaa aauauuacca cgggcccgga aaugaaaagu cccgcaccga cuacggccgg      720

uauagaacaa gcggcgagac ccuggauaac aucuucggga uucuuaucgg aaaaugcacg      780

uuuuacccug acgaguuccg cgcugccaag gcgucuuaua cagcccaaga auuuaaccug      840

cucaaugacc ugaacaaucu uaccgugccc acagagacca aaaagcuguc caaagaacag      900

aagaaccaga uuaucaauua cgucaagaac gagaaagcua ugggcccugc caaacucuuc      960

aaauauaucg cuaagcugcu gagcugugau guggccgaca uuaaagggua ccggaucgau     1020

aagucuggaa aggcggaaau ucauaccuuu gaggccuaua gaaaaaugaa gacacucgaa     1080

acgcuggaca ucgagcaaau ggaucgcgag acccuugaca aacuggcuua cgugcucaca     1140

cugaauaccg aacgggaggg cauucaggaa gcccuugagc acgaguucgc ugaugggucc     1200

uucagccaga agcaggucga cgaacugguu caauuuagaa aagccaacuc cucuaucuuc     1260

ggaaagggcu ggcacaauuu uagcgugaag cucaugaugg agcugauccc ggaacuguac     1320

gagacauccg aggaacagau gaccauucuc acgcgccugg gcaaacagaa gacaaccucu     1380

agcuccaaca aaaccaagua uaucgaugag aaacuucuga cagaagagau uuacaauccc     1440

guggucgcca agagcgugcg gcaggcuauc aagauuguua acgcggccau caaagaauau     1500

ggggacuucg auaauauugu gaucgagaug gcuagagaaa ccaacgagga cgacgagaag     1560

aaagccaucc aaaagauuca gaaagccaau aaggaugaaa aagacgcugc caugcucaag     1620

gcggcuaacc aguacaacgg aaaggccgag cugccucauu cugucuuuca cggccauaaa     1680

cagcuggcca caaagauccg ccuuuggcac caacaggggg aacggugccu cuauacggga     1740

aaaaccauua gcauccacga ucugauuaau aacuccaauc aguucgaggu ggaccauauc     1800

cugccccucu cuaucacauu ugaugacagc cuggcuaaca agguucuugu guacgccacc     1860

gcuaaucagg aaaaaggcca aagaacaccu uaucaggccc uggauuccau ggacgaugcg     1920

uggagcuucc gggagcucaa ggccuuuguc cgcgagucua agacccuguc caacaaaaag     1980

aaagaauacc ugcuuacgga ggaagacauu agcaaguucg augugcggaa aaaguuuauc     2040

gagagaaauc ucgucgacac cagauacgcu ucucgcgugg uucugaacgc ccugcaggaa     2100

cacuuccggg cucauaagau ugauacaaaa guguccgucg ugagagggca auuuaccagc     2160

cagcuccgca gacacugggg caucgagaag acacgggaca ccuaucauca ccacgccguu     2220

gacgcgcuga uuaucgccgc uuccucucag cuuaaucugu ggaaaaagca gaaaaacacg     2280

cucgugagcu acuccgagga ucaacugcug gacaucgaaa caggagagcu uauuucugau     2340

gacgaauaua aggagagcgu cuucaaggcc cccuaccagc auuucgugga uacccucaaa     2400

uccaaggagu uugaagacag cauccuguuc ucuuaucagg uggauuccaa auuuaaucgc     2460

aagauuagcg acgcuacaau cuacgccacc agacaggcca aaguuggcaa ggauaaagcu     2520

gacgagaccu auguccuggg gaagauuaag gauaucuaca cacaagacgg auacgaugcg     2580

uucaugaaaa ucuauaagaa agacaagucu aaauuucuca uguaccggca cgacccgcag     2640

acguucgaaa aggugauuga gccuauccug gaaaacuauc ccaauaagca gauuaacgag     2700

aaaggcaagg aggugccuug uaaucccuuu cuuaaauaca aggaggaaca uggguauauc     2760

cgcaaauacu ccaagaaggg aaacggcccc gaaauuaaaa gccugaagua uuacgauucu     2820

aaacucggga accacaucga cauuaccccu aaggauagca auaacaaagu cgugcugcag     2880

uccguuagcc cguggagagc cgacguguac uucaauaaga caaccggcaa guaugagauc     2940

cugggacuua aauacgcuga ucuccaauuu gaaaagggca cagggaccua uaaaaucucu     3000

caggagaagu acaacgacau uaaaaagaag gagggagucg auuccgacag cgaguucaaa     3060

uuuacgcugu auaagaauga ucugcuccug gugaaagaca cagaaaccaa ggaacagcaa     3120

cuuuuccggu uucugucccg caccaugccc aaacagaagc acuacguuga gcucaaaccu     3180

uaugauaagc agaaguucga aggcggggag gcccugauca aagugcuggg aaacgucgcc     3240

aauucuggcc agugcaagaa agggcuuggc aagagcaaca uuuccaucua caaagugaga     3300

acagacguuc ugggaaauca acauauuauc aagaacgagg gcgacaagcc caaacucgau     3360

uucuga                                                                3366


<210>  3714
<211>  2556
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3714
augaccaacg gcaagauccu ggggcucgac auuggagugg ccagcgucgg cguugggauc       60

auugaggcua aaacaggaaa ggugauccac gccaauucca gacuguucuc ugcggcuaac      120

gccgaaaaua acgccgagcg gcgcggcuuu agaggggcuc ggcgccuuac gagacggaaa      180

aagcaucgcg ugaaaagagu ccgggaucug uucgaaaagu acgacauucu caccgauuuu      240

cgcaaucuga accuuaaucc cuaugagcug agagugaagg gacucacaga acagcugacc      300

aacgaggagc uguucgccgc ucuccggaca aucgccaaac gcagaggcau cagcuaccug      360

gacgaugcgg aagacgauuc caccggcucu agcgacuaug ccaaguccau ugaugagaau      420

cggcgccuuc ugaaaucuau gacaccuggg caaauccagc ucgaaagacu ggagaaguac      480

ggacagcuuc ggggcaacuu uaccguuuau gacgagaaug gggaagcuca cagacugauu      540

aacguguuca gcacguccga uuacaaaaau gaggcccgca agauccucga aacccagagc      600

aacuauaaua agcaaauuac agacgaguuu aucgaagauu acauugagau ccugacccag      660

aaacggaagu acuaucaugg accgggcaac gagaaaucua gaacagacua cgggcgcuuc      720

cggaccgacg gaacgacacu ggaaaacauc uuuggcauuc ucaucggcaa gugcuccuuc      780

uaucccgagg aauacagagc uagcaaagcc ucuuauaccg cgcaggaguu uaauuuccug      840

aacgaucuua auaaccugaa ggucccuaca gaaaccggga aacucuccac cgagcagaag      900

gaguaccugg ugaauuuugc caagaacagc aaaacacuug gagcuucuaa gcugcucaaa      960

gaaauugcca agcugguuga cggcgaugug aaagacaucu ccggguaucg cgaggauagc     1020

aagggaaagc ccgaccugca cacguucgaa ccuuaccgga aacucaaguu caaucugacc     1080

acagucgaua uugacaaccu uuccagagau auccuggaca aacucgcuga uauccugacc     1140

cuuaauacag agcgcgaagg cauugaggac gccaucaacc aaaaucugcc cgagcaguuu     1200

accaaggaac agauuucuga gaucgugcag auucggaaaa gccaauccag cgccuucaac     1260

aagggguggc auucuuuuuc cgcuaagcuc augaaugaac ugaucccuga gcuguacgcg     1320

acgagcgagg aacagaugac aauccucacc agacuggaga aauucaaggc caccaaaaag     1380

ucuagcaaaa acacaaagac cauugacgaa aaggagauca cagaugaaau uuauaauccg     1440

guggucgcua aauccguucg ccagacgauc aagauuauca acgccgccgu gaaaaaguac     1500

ggagacuuug auaaaauugu gaucgagaug ccccgggaca agaacgcuga ugacgagaaa     1560

aaguucaucg auaagaaaaa uaaggaaaac aaaaaggaga aagacgauuc ucuuaagaga     1620

gccgcguauc uguacaaugg caccgacaag cucccugaug acgucuuuca cggcaacaaa     1680

caacuggcua caaagauucg gcugugguau cagcaggggg aacgcugucu uuacagcgga     1740

aaacccaucc ucauucagga ucuggugcac aauuccaaca auuucgagau cgaccauauu     1800

cugccucuca gccugucuuu ugacgauucc cuugccaaca agguucuggu guaugccugg     1860

gcuaaucaag aaaaagagca gaagaccccc uaccagguca ucgacagcau ggaugccgcu     1920

uggucuuuca gagagaugaa ggacuacgug cucaaacaga agggcaucgg gaaaaagaaa     1980

cgggaauauc ugcugacaac cgagaacauu gauaagaucg aaguuaagaa aaaguuuauu     2040

gagagaaauc uuguggacac gcgcuacgcc ucccgggucg ugcucaacag ccugcaaacc     2100

gcgcugaaag aacucggaaa ggauacaaaa gucuccgugg uuagaggcca guucaccucu     2160

cagcugcgca gaaaguggaa aaucgacaag agccgggaga cauaucacca ucacgccgug     2220

gaugcucuua uuaucgccgc uuccucucag cugaagcucu ggcaaaaaca cgagaaucug     2280

auguuugaaa acuacgggga gaaucagguc gugaacaagg aaaccggcga gauccugagc     2340

auuuccgacg augaguauaa agaaagcguu uuccagccgc cuuaccaggg auucgugaac     2400

acgaucucuu ccaaggccuu ugaggacgaa auucuuuuca gcuaucaagu cgauucuaaa     2460

uuuaaucgca agguguccga cgcgacaauc uacagcacca gaaaggccaa acucggcaag     2520

gacaaaaagg aggaaacaua cgugcugggg aaauga                               2556


<210>  3715
<211>  2598
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3715
augagacaca agaucggccu ggacauuggg aucaccagcg ugggaugggc cgucauuaac       60

cucgauaucc cccggauuga ggaccugggc guucgcaucu ucgauagagc ugaaaauccu      120

cagacagggg agucccuugc ccugccgcgg cgccucgcga gaucugcucg gcgcagacug      180

cggcgcagaa aacaucggcu ugaacgcauc agacggcugc ucauucgcga gggaauccug      240

acgaaggagg aacuggacaa acucuuugag gaaaagcacg agauugaugu guggcaacug      300

agaguggaag cccuugaccg gaaacugaac aaugaugagc ucgccagagu ccugcuucau      360

cuggcuaagc gccggggcuu caagagcaac agaaaauccg agcgcaauaa caaggaaaau      420

ucuaccaugc ucaaacacau cgaggaaaac cgggcccugc ugagcgggua cagaacagug      480

gcugagauga uuguuaagga ccccaaauuu gccuuccaua agcgcaauaa gggagaaaac      540

uauaccaaua caaucgcgcg ggaugaccuc gagagagaga uuaaacugau cuuuaccaag      600

cagcgcgaau acggcaacau cgugugcaca gagaccuucg aaaaugagua uauuaugauc      660

ugggccuccc agcggccuuu ugcuucuaaa aacgauauug aaaagaaagu cggcuucugu      720

acguuugagc ccaaggagaa aagagccccu aaggcuaccu acacauucca gagcuuugug      780

gccugggaac acaucaauaa gcugcgccug guuuauccca ccgggacacg gggacucacc      840

gacgaggaaa gacggcugcu uuacaaacaa gcguuccaca agaacaaaau uacguaucau      900

gauauccgca cacugcucca ccuuccugac gacaccuacu uuaagggcau cguguacgau      960

agaggggccc cgcugaaaca guccgagaca auucgguucc ucgaacugga cgcuuaucau     1020

cagaucagaa aggccgucga uaagguguac ggaaaaggca agucuagcuc cuuccuuccc     1080

auugacuuug auaccuucgg guaugcucug acccucuuua aagacgaugc cgacauccgc     1140

agcuaccugc ggaacgagua ugagcagaau ggaaagagaa ugccuaaccu ugccaauaaa     1200

guguacgaua acgaacccau ugaggaacug cucaaucugu cuuucacaaa guuuggccac     1260

cugucccuca aggcucugcg caacauccuu ccuuauaugg agcaaggcga ggucuacagc     1320

guugcgugcg aaagagccgg guacacguuc accggaccca aaaagaaaca gaagacacug     1380

cuccugccga auaucccucc cauugcuaac ccugugguga ugcgggcccu gacccaggcc     1440

cgcaaagucg ugaaugcuau cauuaagaag uauggcucuc ccguuuccau ccacauugag     1500

cuugccagag accucagcca gacauuugau gaacggcgca aaaccaagag agagcaagac     1560

gaaaaccgga aaaagaauga gacggcgauc cgccagcuga uggaguacgg gcugacaauu     1620

aaccccaccg gacaugacau cgugaaauuc aagcucuggu ccgaacagaa uggcagaugu     1680

gcuuauucuc ugcaaccuau cgagauugaa cggcuucugg agccggggua cgucgaagug     1740

gaucacguua uccccuauag ccgcucccuc gacgauagcu acaccaacaa agugcugguc     1800

cugacaaagg agaauagaga gaagggaaac cggauuccug ccgaauaucu uggcgugggc     1860

accgagagau ggcagcaguu uaaaacauuc guccucacga acaagcaguu uucuaaaaag     1920

aaacgcgacc ggcugcugag acuccauuac gaugaaaaug aggaagccga guggaagaac     1980

cgcaaucuga acgacacccg guacaucucc agauucuuug cuaauuucau ucgcgagcac     2040

cuuaaguucg ccgaaagcga ugacaaacaa aagguguaua caguuaacgg gcgggugacc     2100

gcucaucuga gaucucgcug ggauuuuaau aaaaaccggg aggaaagcga ccuccaccac     2160

gccgucgaug cggccaucgu ggcuugcaca ucccccucug acaucgccag aguuaccgcu     2220

uucuaccagc ggcgcgagca guguaaggag agcgccaaaa aggcggaacc ucauuuuccc     2280

cagccguggc cucacuucgc cgaugagcug agagcucggc uguccaagaa ucccaaagaa     2340

agcauuaagg cccuuaaccu cggaaauuau gacgaccaaa aacuggaguc ucugcagccu     2400

guguuugucu cccgcaugcc caagagaagc gugacgggcg cugcccauca ggaaacccuc     2460

cggagauaca ucgggauuga ugagcgcucu ggaaaaaucc agacaguggu uaagaccaag     2520

cuguccgaga uuaaacuuga cgaaacaggc cacuucccua uguaugggaa ggagagcgau     2580

cccccggccu ccgcuuga                                                   2598


<210>  3716
<211>  2886
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3716
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcucgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacaa uggcgacgag      420

gaaaaaggga agauucugaa ggcuaucaaa cagaacgagg agaaacuugu uaauuaucag      480

uccgugggag aauaccugua uaaagaguac uuucagaagu ucaaagaaaa cagcaaggag      540

uuuauuaaug ugagaaacaa aaaggaaucu uacgagcggu guaucacgca auccuuccuc      600

aaguaugagc ugaaacugau uuuucagaag cagcgcgaau uuggcuuuag cuucucuaaa      660

aaguucgagg aagagguccu cuccguggcc uuuuacaaaa gagcgcugaa ggauuucagc      720

caucuuguug gcaauugcuc uuucuucucc gacgaaaagc gggcccccaa aaacagcccu      780

cuggcuuuua uguucguggc ccucaccaga aucaucaauc ugcuuaacaa ccugaagaau      840

acagagggga uucucuauac caaagaugac cugaacgcuc ugcucaauga gguccugaag      900

aacggaacac uuaccuacaa acagacaaag aagcugcucg gccuguccga ugacuaugaa      960

uuuaaagggg agaagggaac cuacuucauc gaauuuaaaa aguauaaaga guucauuaag     1020

gcccuuggcg aacacaaucu gucucaagac gaucucaacg agaucgccaa ggacauuacg     1080

cugaucaaag augagaucaa gcugaaaaag gcucucgcga aauacgaccu gaaucagaac     1140

cagauugaua gccuuuccaa gcuggaauuu aaagaccauc ucaauaucgg guucaaggcc     1200

cugaagcuga uuacaccccu uaugcucgag ggaaagaagu acgaugaggc uuguaacgaa     1260

cugaaucuga aaguggccau caacgaggac aagaaagauu uucucccugc cuucaaugaa     1320

accuauuaca aggacgaggu uaccaacccc gugguccugc gcgcuauuaa ggaauaucgg     1380

aaagugcuua augcccugcu caagaaauac ggcaaggugc acaaaaucaa caucgagcug     1440

gcgagagaag ucggcaagaa ccacagccag cgcgcuaaga uugagaaaga gcaaaaugaa     1500

aacuauaagg ccaaaaagga ugccgagcug gaaugcgaga aacuugggcu caagaucaau     1560

ucuaagaaca uucugaaacu gagacucuuc aaggaacaga aagaguuuug ugcuuacucc     1620

ggagagaaga ucaaacugag cgaccuucag gacgaaaaga ugcuggagau ugaucauauc     1680

uauccuuacu cucggagcuu cgacgauucc uacaugaaua aaguucucgu guuuacaaag     1740

caaaaccagg aaaagcugaa uaaaaccccg uucgaggccu uuggcaacga caaugugaag     1800

uggcagaaaa uugaaguccu ggcuaagaac cuucccgaga aaaagcagaa gcgcauccuc     1860

gauaaaaauu auaaggacaa agagcaaaag gauuucaaag accggaaccu gaaugauacg     1920

agauacaucg cccgccuggu gcucaacuau acaaaggacu accuggauuu ucuuccucug     1980

ucugacgaug aaaauaccaa gcucaaaggg agcaagguuc acguggaggc gaaauccgga     2040

augcugacaa gcgcccugcg gcauaccugg ggcuucucua ccaaggacag aaacaaccac     2100

cuucaccaug cuauugacgc cgcuaucauu gccuaugcga auaacuccau cgucaaagau     2160

uuuagcgacu ucaagaagga acaggagucu aauuccgccg agcucuacgc uaaaaagauu     2220

agcgaacugg auuauaaaaa caagcgcaaa uuuuucgagc ccuuuuccgg guuccggcag     2280

aaggugcugg acaagaucga ugaaaucuuc guuucuaaac cugagagaaa gaaacccagc     2340

ggagcccucc acgaagagac auuucggaag gaggaagagu ucuaccaguc cuacggcggc     2400

aaagaagggg ugcugaaggc ucuugagcug ggaaaaauuc gcaaggucaa uggcaagauc     2460

gugaaaaacg gggacauguu uagagucgau auuuucaagc auaaaaagac gaauaaauuu     2520

uaugccgugc cgaucuacac cauggacuuc gcccucaagg uucugccuaa caaggcugug     2580

gcgcggucua aaaagggaga aauuaaagau uggauccuga uggacgagaa uuaugaguuu     2640

ugcuucagcc uuuacaagga uucccugauc cucauucaaa caaaagacau gcaggaaccc     2700

gagcuggucu auuuuaacgc cuucaccagc ucuacagugu cccucaucgu uagcaagcac     2760

gauaauaagu uugaaacccu gucuaaaaac cuuaaggugu ucgagaaaua cauugucucc     2820

gcucugggcg aagugacgaa ggccgaguuc agacagcgcg aggacuuuaa aaaugaacuc     2880

cgguga                                                                2886


<210>  3717
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3717
auggccaaga uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaagacug cggagugaga aucuucacca aggcggagaa ucccaaaaca      120

ggcgaaagcc uggcccuucc ucggcgcauu gcuagauccg cccggaagcg ccuggcuaga      180

cggaaagccc gccucaacca ccugaagcau cuuaucgcua augaguuuaa gcugaacuac      240

gaagauuauc agucuuucga cgagauccuc gccaaagcgu acaaggggag ccugauuucc      300

ccguaugagc ugagauuucg ggcccucaau gaacugcuuu cuaaacaaga uuucgcucgc      360

gucauccugc acauugccaa gagacgggga uacgacgaua ucaaaaacaa uggcgacgag      420

gaaaagggga agauucucaa agcuaucaag cagaacgagg agaaacuggc caauuaucag      480

agcguuggag aauaccuuua uaaggaguac uuucagaaau ucaaggaaaa cuccaaagag      540

uuuauuaaug ugcgcaacaa gaaggaaagc uacgagagau guaucgcgca aucuuuccug      600

aaagaugagc ucaagcugau cuuuaaaaag cagcgggagu ucggcuuuuc cuucagcaaa      660

aaguucgaag aagaggugcu gucugucgcc uuuuauaagc gcgcucucaa agacuucucc      720

caucuggugg gcaauugcag cuuuuucacg gaugaaaaga gagcccccaa aaacucuccu      780

cuugcuuuua uguucguugc ccugacccgg auuaucaauc uccugaacaa ccuuaagaau      840

acacuguaca ccaaagacga ucucaacgcc cugcugaaug aggugcucaa gaacgggaca      900

cugaccuaua agcagacaaa gaaacuucug ggacucuccg acgacuacga guuuaaaggc      960

gaaaagggga auuauuucau ugaguuuaaa aaguacaagg aguucaucaa agcucuggga     1020

gaacacagcc uuucccagga ugaccugaac gaaauugcga aggauaucac ccucaucaaa     1080

gacgagauua agcugaaaaa ggcccuggcu aaauaugauc ucaaugagaa ccaaaucgac     1140

aaucugucua aacuugaauu uaaggaucau cugaacauua gcuucaaagc ccucaagcug     1200

aucacgcccc ugaugcuuga gggcaaaaag uacgacgaag ccuguaauga gcucacccug     1260

aaagucgcua uuaacgagga uaagaaggac uuucugccug ccuucaauga aacauacuau     1320

aaagaugagg ugaccaaccc cguugugcuc agagcgauca aggaauaccg caaaguccug     1380

aaugcccuuc ugaagaaaua ugggaaggug cacaagauca acauugagcu cgcucgggaa     1440

gugggaaaga accacuccca gagagccaag aucgagaaag agcagaauga aaacuacaag     1500

gcuaaaaagg acgccgagcu ggaaugcgag aagcugggcc uuaaaauuaa uagcaagaac     1560

auccucaaac ugcgccuguu caaggaacag aaagaguuuu gugucuauuc uggcgagaag     1620

auuaaacucu ccgaccugca agaugaaaag augcuugaga ucgaccauau uuacccuuau     1680

agccggucuu ucgaugacag cuacaugaau aagguucugg uguuuacaaa acagaaccag     1740

gaaaagcuca aucaaacccc guucgaggcu uuugggaacg auuccgccaa auggcagaag     1800

aucgaagugc uggcgaaaaa ucugcccacg aagaagcaga aaagaauccu ugacaagaac     1860

uacaaagaua aggagcagaa agacuucaag gaucgcaauc ucaacgacac acgguauauu     1920

gccagacugg uccugaauua caccaaggau uaucucgacu uucugccucu uucugaugac     1980

gagaacacaa aacugaauga cacccaaaag ggaagcaaag ugcacguuga agcuaagucc     2040

ggcaugcuca ccagcgcccu gcgccauaca uggggguucu cuacgaaaga ucggaacaac     2100

caucugcacc acgcuaucga cgccgugauu aucgcguacg ccaauaacuc cauugucaag     2160

gcuuuuagcg auuucaagaa agagcaggaa ucuaauaccg ccgagcuuua ugcuaagaaa     2220

aucuccgagc ucgacuacaa gaacaaaaga aaguuuuucg aacccuuuag cggauuccgc     2280

cagaaggugc uggauaaaau cgacgagauu uucguuucca agccugaacg gaaaaagccc     2340

ucuggcgccc ugcacgagga aacauuuaga aaagaggagg aguucuauca gagcuacggg     2400

ggaaaggaag gcgugcucaa agcccuggaa cuuggcaaga uccggaaggu caaugggaaa     2460

auugugaaga acggagauau guuucgcguc gacaucuuca aacauaagaa aaccaauaag     2520

uuuuacgcug ugccgauuua uacaauggau uucgcgcuga agguucuccc uaacaaagcc     2580

guggcuagau ccaagaaagg cgagaucaag gacuggaucc ugauggauga aaauuacgag     2640

uuuugcuucu cucuguauaa agacagccuu auucugaucc aaaccaagga uaugcaggag     2700

cccgaauuug ucuacuauaa cgccuucacg uccagcacag ugucucucau uguuuccaag     2760

cacgacaaua aauuugagac ccugagcaag aaccagaaaa uccucuucaa gaaugccaac     2820

gaaaaagagg ugauugcuaa gucuaucggg auccagaauc ugaaggucuu cgaaaaauac     2880

auuguguccg cccuuggaga ggugaccaag gcggaguuuc ggcaaagaga agaguucaaa     2940

aacgaacugc gcuga                                                      2955


<210>  3718
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3718
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcucgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaaccg gcuuaaacac cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgcuuuag agcccugaau gaacuucuga gcaagcaaga uuucgcucgg      360

gucauccucc auauugccaa acgcagagga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaaacuugc gaacuaucag      480

agcguugggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugcggaauaa aaaggagucc uacgaacgcu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caaagagagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcgucgcu uuuuauaagc gggcccugaa ggauuucucu      720

caccuugugg gcaacugcuc cuuuuucacc gacgagaaaa gagcucccaa gaauagcccu      780

cuggccuuua uguucguugc ccucacacgc auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugcuc ugcucaacga guuucugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uucaagggcg aaaaagggac guauuuuauu gaguucaaga aauacaagga auuuaucaag     1020

gcgcuuggag agcauaaccu gucucaggac gaucucaaug aaauugccaa agacaucacc     1080

cugauuaagg augagaucaa acugaagaaa gcucucgcca aguaugaccu gaaccaaaau     1140

cagaucgaua gccuuuccaa acuggaguuc aaggaccacc ucaacauuag cuuuaaggcc     1200

cugaaacuga ucacaccccu uaugcucgaa ggcaagaaau acgaugaggc uuguaaugaa     1260

cugaaccuga agguggccau uaaugaggac aaaaaggauu uccucccugc guucaacgag     1320

accuacuaua aggacgaagu cacaaauccc gugguucugc gggcuaucaa agaguacaga     1380

aaggugcuua acgcccugcu caaaaaguau gggaaagucc auaagauuaa uaucgaacug     1440

gcccgcgagg ugggaaagaa cuacucucag cgggcuaaaa ucgaaaagga gcagaacgag     1500

aauuauaaag ccaagaaaga cgcugaacug gagugcgaaa aacuuggccu caagauuaac     1560

uccaaaaaua uccugaagcu gagacucuuu aaagagcaaa aggaguucug ugccuacagc     1620

ggcgagaaaa uuaagaucuc ugaucugcag gacgagaaaa ugcuugaaau ugaucacauc     1680

uauccuuaca gccgcuccuu ugacgauucu uacaugaaca aggugcuggu cuucaccaag     1740

cagaaucaag agaaacucaa ccagacgccg uuugaagcgu ucgggaauga cagcgccaag     1800

uggcagaaaa uugagguucu ggcuaagaac cugcccacaa agaagcagaa gcggauccuu     1860

gauaaaaauu auaaggacaa agaacaaaag gauuuuaaag acagaaaccu caaugauacc     1920

cgcuacaucg cccggcuggu gcugaacuau acaaaggacu accucgauuu ccugccucuu     1980

uccgacgacg agaauaccaa gcugaacgau acccacaaag gaagcaaggu gcaugucgag     2040

gcuaaaucug gcaugcucac auccgcccug agacacacgu ggggguuuag caccaaggac     2100

cgcaauaacc aucugcggca cgcgauugau gccgugauca uugcuuaugc caacaauucu     2160

aucguuaagg cuuucuccga cuuuaagaag gaacaggaga gcaacuccgc cgaacuuuac     2220

gccaaaaaga uuucugagcu cgauuauaaa aauaagagaa aauucuuuga gcccuucagc     2280

ggauuccggc agaaggugcu ggacaagauc gaugaaaucu uugucuccaa accugagcgc     2340

aagaaacccu cuggcgcucu gcacgaagag acauucagaa aggaagagga guuuuaccag     2400

agcuacgggg gaaaagaagg cgugcucaag gcgcuggagc uuggcaaaau ucggaagguu     2460

aacgggaaga ucgugaaaaa uggagacaug uucagagucg auauuuuuaa gcauaaaaag     2520

accaacaaau ucuaugccgu gccgaucuac acaauggacu uugcucugaa gguccucccu     2580

aauaaggccg uggcccgcuc caaaaagggc gaaauuaaag auuggauccu gauggacgag     2640

aacuaugagu ucugcuuuag ccuguacaag gauucucuua uccugauuca aaccaaagac     2700

augcaggaac ccgaguucgu uuauuacaau gcuuuuacgu ccagcacagu gucucucauc     2760

gucuccaagc acgacaacaa guucgaaacc cugagcaaaa aucagaagau ucucuucaaa     2820

aacgccaaug agaaggaagu gaucgcgaaa ucuauuggga uccagaaccu gaagguuuuu     2880

gagaaguaua ucgugagcgc ucuuggagaa gucaccaaag ccgaguuccg gcaaagagag     2940

gauuuuaaga aauga                                                      2955


<210>  3719
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3719
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagucgagaa ucccaagaca      120

ggcgaaagcc uggcgcuucc ucgcagacug gcucgguccg cccgcaaaag acucgcccgg      180

cgcaaggcua gacugaacca ccuuaaacau cugauugcca augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gcuaaggccu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcgcugaau gaacuucuga gcaagcaaga uuucgccaga      360

guuauccucc acauugcuaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg ccauucugaa ggcuaucaaa cagaaugaag agaaacuugc caacuaucag      480

agcguggggg aguaccugua uaaggaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugcgca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaaguccu cagcguggcc uuuuauaaga gagcucugaa ggauuucucu      720

caucuuguug gcaacugcuc cuuuuucacc gacgagaaac gggcccccaa gaauagcccu      780

cuggcuuuua uguucgugac acucaccaga auuaucaacc ugcuuaauaa ccugaaaaac      840

acagaaggca uccucuacac caaggaugac cugaaugccc ugcucaacga gguccugaaa      900

aaugggacac uuaccuauaa gcagacgaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac cuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcccuuggag agcacaaccu gucucaggac gaucucaaug aaauugcuaa agacaucaca     1080

cugauuaagg augagaucaa acugaagaaa gcgcucgcca aguaugaccu gaaccaaaau     1140

cagaucgaua gccuuuccaa acuggaguuu aaggaccauc ucaacauuag cuucaaggcu     1200

cugaaacuga ucacaccccu uaugcucgaa ggcaagaaau acgaugaggc cuguaaugaa     1260

cugaaccuga agguggccau uaaugaggac aaaaaggauu uucucccugc uuucaacgag     1320

accuacuaua aggacgaagu uaccaauccc gugguccugc gcgccaucaa agaguaccgg     1380

aaggugcuua acgcgcugcu caaaaaguau gggaaagugc acaagauuaa uaucgaacug     1440

gcuagagagg ucggaaagaa ccacucucag cgcgccaaaa ucgaaaagga gcagaacgag     1500

aauuacaaag ccaagaaaga cgcugaacug gagugcgaaa aacuuggccu caagauuaac     1560

uccaagaaua uccugaagcu gagacucuuc aaagagcaaa aggaauuuug ugccuauagc     1620

ggcgagaaaa uuaagaucuc ugaucugcag gacgagaaaa ugcuugaaau ugaucauauc     1680

uacccuuaua gcagauccuu cgacgauucu uacaugaaca agguucuggu guuuacgaag     1740

cagaaucaag agaaacucaa caagacaccg uucgaggcuu uugggaauga cagcgccaaa     1800

uggcagaaga uugaagugcu ggcgaaaaac cugcccacca agaagcagaa acgcauccuu     1860

gauaagaauu acaaagacaa ggaacagaaa gauuucaagg accggaaccu caaugauaca     1920

agauauaucg cccgccuggu ccugaacuac accaaggacu aucucgauuu ucugccucuu     1980

uccgacgacg agaauaccaa acugaacgau acacaaaagg gaagcaaagu gcacguugag     2040

gcuaagucug gcaugcucac guccgcccug cggcauaccu ggggguucag cacaaaagac     2100

agaaauaacc aucugcacca cgcuauugau gccgugauca uugcguacgc caacaauucu     2160

aucgucaagg cuuuuuccga cuucaagaaa gaacaggaga gcaacuccgc cgaacuuuau     2220

gcuaagaaaa uuucugagcu cgauuacaag aauaaacgca aguuuuucga gcccuuuagc     2280

ggauuccggc agaaggugcu ggacaaaauc gaugaaaucu ucguuuccaa gccugagcgg     2340

aaaaagcccu cuggcgcccu gcacgaagag accuuucgga aagaagagga guucuaucag     2400

agcuacgggg gaaaggaagg cgugcucaaa gcccuggagc uuggcaagau ucgcaagguc     2460

aacgggaaaa ucgugaagaa uggagacaug uuuagagucg auauuuucaa acauaagaaa     2520

acaaacaagu uuuacgcugu gccgaucuau accauggacu ucgcgcugaa gguucucccu     2580

aauaaagccg uggcucgguc caagaaaggc gaaauuaagg auuggauccu gauggacgag     2640

aacuacgaau uuugcuucag ccuguauaaa gauucucuua uccugauuca aacgaaggac     2700

augcaggagc ccgaguuugu cuacuauaau gccuucacau ccagcaccgu gucucucauc     2760

guuuccaagc acgacaacaa auuugaaacc cugagcaaga aucagaaaau ucucuucaag     2820

aacgccaaug agaaagaagu gaucgcuaag ucuauuggga uccagaaccu gaaggucuuc     2880

gagaaauaca ucgugagcgc ccuuggagaa gugacaaagg cggaguuuag acaacgcgag     2940

gauuucaaaa aguga                                                      2955


<210>  3720
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3720
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcgcuucc ucgcagacug gcccgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaaacuugc gaacuaucag      480

agcguugggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcgucgcu uuuuauaaga gagcccugaa ggauuucucu      720

caucuugugg gcaacugcuc cuuuuucacc gacgagaaac gggcucccaa gaauagcccu      780

cuggccuuua uguucguugc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugcuc ugcucaacga ggugcugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac guauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcgcuuggag accacucucu gagccaggau gaccucaacg agauugccaa agauaucacc     1080

cugauuaagg acgaaaucaa acugaagaaa gcucucgcca aguaugaucu gaaucaaaac     1140

cagaucgacu cccuuagcaa acuggaguuu aaggaucauc ucaauauuuc uuucaaggcc     1200

cugaaacuga ucacaccccu uaugcucgag ggcaagaaau acgacgaggc uuguaacgaa     1260

cugaaucuga aggucgccau uaacgaagau aaaaaggacu uucucccugc guucaaugag     1320

accuacuaua aggacgaggu gacaaacccc guugugcugc gcgcuaucaa agaauaccgg     1380

aagauucuua augcccugcu caaaaaguau gggaaagucc acaagaucaa caucgagcug     1440

gccagagaag ugggaaagaa ucacucccag cgcgcuaaaa uugagaagga acagaacgag     1500

aacuacaaag ccaagaaaga ugcugagauc gaaugcgaga agcugggccu uaagauuaau     1560

agcaaaaaca uccucaagcu gcggcuguuc aaagaacaaa aggaguuuug ugccuauucu     1620

ggcgaaaaga uuaagcucag cgaccugcag gaugagaaaa ugcuugagau cgaccauauu     1680

uacccuuauu ccagaucuuu cgaugacagc uacaugaaua aggugcuggu cuuuaccaag     1740

cagaaccaag aaaaacucaa uaagacgccg uucgaggcgu uugggaacga uuccacaaaa     1800

uggcagaaga ucgaaguucu ggccaaaaau cugcccgaga agaagcagaa acgcauccuu     1860

gacaagaacu acaaagauaa ggaacagaaa gacuucaagg aucggaaucu caacgacacc     1920

agauauauug cucgccuggu gcugaauuac acaaaggauu aucucgacuu ucugccucuu     1980

agcgacgaug agaacaccaa acugaaugac acccaaaagg gaucuaaagu gcacgucgag     2040

gccaaguccg gcaugcucac aagcgcucug cggcauacgu ggggguucuc ugccaaagau     2100

agaaacaauc aucugcacca cgcgaucgac gccgcuauua ucgccuacgc uaacaacucc     2160

auugugaagg ccuuuagcga uuucaagaaa gaacaggagu ccaauucugu ugaacuuuau     2220

gccaagaaaa ucagcgagcu cgacuacaag aacaaacgca aguuuuucga gcccuuuucc     2280

ggauuccggc agaaggugcu ggauaaaauc gacgaaauuu ucgucucuaa gccugagcgg     2340

aaaaagccca gcggcgcucu gcacgaagag accuuuagaa aagaagagga guucuaucag     2400

uccuacgggg gaaaggaagg cgugcucaaa gcgcuggagc uuggcaagau ccgcaagguu     2460

aaugggaaaa uugugaagaa cggagauaug uuuagagucg acaucuucaa acauaagaaa     2520

acaaauaagu uuuacgccgu gccgauuuau accauggauu ucgcucugaa gguccucccu     2580

aacaaagccg uggcccggag caagaaaggc gaaaucaagg acuggauccu gauggaugag     2640

aauuacgaau uuugcuucuc ucuguauaaa gacucccuua uucugaucca aacaaaggac     2700

augcaggagc ccgagcucgu uuacuuuaac gcuuucacca gcucuacggu gucccugauu     2760

gucagcaagc acgauaauaa auuugaaaca cucucuaaga accagaaaau ccuguucaag     2820

aaugccaacg agaaagaagu gauugcuaag agcaucggga uccagaaucu uaagguuuuc     2880

gagaaauaua uuguguccgc ucugggagaa gucaccaagg ccgaguuuag acaacgcgag     2940

gacuucaaaa aguga                                                      2955


<210>  3721
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3721
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagucgagaa ucccaagaca      120

ggcgaaagcc uggcgcuucc ucgcagacug gcucgguccg cccgcaaaag acucgcccgg      180

cgcaaggcua gacugaacca ccuuaaacau cugauugcca augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gcuaaggccu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcgcugaau gaacuucuga gcaagcaaga uuucgccaga      360

guuauccucc acauugcuaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg ccauucugaa ggcuaucaaa cagaaugaag agaagcucgc caacuaucag      480

agcguggggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugcgca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaaguccu cagcguggcc uuuuauaaga gagcucugaa ggauuucucu      720

caucuuguug gcaacugcuc cuuuuucacc gacgagaaac gggcccccaa gaauagcccu      780

cuggcuuuua uguucguggc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugccc ugcucaacga gguccugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac guauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcucuuggag agcacaaccu gucucaggac gaucucaaug aaauugcgaa agacaucacc     1080

cugauuaagg augagaucaa acugaagaaa gcccucgcua aguaugaccu gaaccaaaau     1140

cagaucgaua gccuuuccaa acuggaguuu aaggaccauc ucaacauuag cuucaaggcc     1200

cugaaacuga ucacaccccu uaugcucgaa ggcaagaaau acgaugaggc cuguaaugaa     1260

cugaaccuga agguggcuau uaaugaggac aaaaaggauu uucucccugc cuucaacgag     1320

accuacuaua aggacgaagu uacaaauccc gugguccugc gcgcgaucaa agaguaccgg     1380

aaggugcuua acgcucugcu caaaaaguau gggaaagugc acaagauuaa uaucgaacug     1440

gccagagagg ucggaaagaa ccacucucag cgcgccaaaa ucgaaaagga gcagaacgag     1500

aauuacaaag cuaagaaaga cgccgaacug gagugcgaaa aacuuggccu caagauuaac     1560

uccaaaaaua uccugaagcu gcggcuuuuc aaagagcaaa aggaauuuug ugcuuauagc     1620

ggcgagaaaa uuaagaucuc ugaucugcag gacgagaaaa ugcuugaaau ugaucauauc     1680

uacccuuaua gcagauccuu cgacgauucu uacaugaaca agguucuggu guuuaccaag     1740

cagaaucaag agaaacucaa caagacgccg uucgaagccu uugggaauga cagcgcgaaa     1800

uggcagaaga uugaggugcu ggccaaaaac cugcccacaa agaagcagaa acgcauccuu     1860

gauaagaauu acaaagacaa ggaacagaaa gauuucaagg accggaaccu caaugauacc     1920

agauauaucg cucgccuggu ccugaacuac acaaaggacu aucucgauuu ucugccucuu     1980

uccgacgacg agaauaccaa acugaacgau acccaaaagg gaagcaaagu gcacguugag     2040

gccaagucug gcaugcucac auccgcucug cggcauacgu ggggguucag caccaaagac     2100

agaaauaacc aucugcacca cgccauugau gcggugauca uugccuacgc uaacaauucu     2160

aucgucaagg ccuuuuccga cuucaagaaa gaacaggaga gcaacuccgc ugaacuuuau     2220

gccaagaaaa uuucugagcu cgauuacaag aauaaacgca aguuuuucga gcccuuuagc     2280

ggauuccggc agaaggugcu ggacaaaauc gaugaaaucu ucguuuccaa gccugagaga     2340

aagaagcccu cuggcgcccu gcacgaagag acauuucgga aagaagagga guucuaucag     2400

agcuacgggg gaaaggaagg cgugcucaaa gcucuggagc uuggcaagau ucgcaagguc     2460

aacgggaaaa ucgugaagaa uggagacaug uuuagagucg auauuuucaa acauaagaaa     2520

accaacaagu uuuacgcggu gccgaucuau acaauggacu ucgcccugaa gguucucccu     2580

aauaaagcug uggcccgguc caagaaaggc gaaauuaagg auuggauccu gauggacgag     2640

aacuacgaau uuugcuucag ccuguauaaa gauucucuua uccugauuca aaccaaggac     2700

augcaggagc ccgaguuugu cuacuauaau gccuucacgu ccagcacagu gucucucauc     2760

guuuccaagc acgacaacaa auuugaaacc cugagcaaga aucagaaaau ucucuucaag     2820

aacgcuaaug agaaagaagu gaucgccaag ucuauuggga uccagaaccu gaaggucuuc     2880

gagaaauaca ucgugagcgc gcuuggagaa gugaccaagg cugaguuuag acaacgcgag     2940

gauuucaaaa aguga                                                      2955


<210>  3722
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3722
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcucgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaaacuugc gaacuaucag      480

agcguugggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcgucgcu uuuuauaaga gagcccugaa ggauuucucu      720

caucuugugg gcaacugcuc cuuuuucacc gacgagaaac gggcucccaa gaauagcccu      780

cuggccuuua uguucguugc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugcuc ugcucaacga ggugcugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac guauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcgcuuggag accacucucu gagccaggau gaccucaacg agauugccaa agauaucacc     1080

cugauuaagg acgaaaucaa acugaagaaa gcucucgcca aguaugaucu gaaucaaaac     1140

cagaucgacu cccuuagcaa acuggaguuu aaggaucauc ucaauauuuc uuucaaggcc     1200

cugaaacuga ucacaccccu uaugcucgag ggcaagaaau acgacgaggc uuguaacgaa     1260

cugaaucuga aggucgccau uaacgaagau aaaaaggacu uucucccugc guucaaugag     1320

accuacuaua aggacgaggu gacaaacccc guugugcugc gcgcuaucaa agaauaccgg     1380

aagguccuua augcccugcu caaaaaguau gggaaagugc acaagauuaa caucgagcug     1440

gccagagaag ugggaaagaa uuacucccag cgcgcuaaaa ucgagaagga acagaacgag     1500

aacuauaaag ccaagaaaga ugcugagcug gaaugcgaga aacuuggccu caagauuaau     1560

agcaaaaaca uccugaagcu gagacucuuc aaagaacaaa aggaguuuug ugccuacucu     1620

ggcgaaaaga uuaagaucag cgaccugcag gaugagaaaa ugcuugagau ugaccacauc     1680

uauccuuacu cccggucuuu cgaugacuuu uacaugaaua agguccuggu uuucaccaag     1740

cagaaccaag aaaaacucaa ucagacgccg uuugaggcgu ucgggaacga uagcgccaag     1800

uggcagaaaa uugaagugcu ggcuaagaau cugcccacaa agaagcagaa gcgcauccuu     1860

gacaaaaacu auaaggauaa agagcaaaag gacuuuaaag aucggaaucu caacgacacc     1920

agauacaucg cccgccuggu gcugaauuau acaaaggauu accucgacuu ccugccucuu     1980

uccgacgaug aaaacaccaa gcugaaugac acccagaaag gaagcaaggu ccauguggag     2040

gcuaaaucug gcaugcucac auccgcccug cggcacacgu ggggguuuag cgcgaaggau     2100

agaaacaauc aucugcacca cgccauugac gcuguuauca uugccuaugc uaacaacucu     2160

aucgugaaag ccuucuccga uuuuaagaag gagcaggaaa gcaauuccgc cgagcuuuac     2220

gcuaaaaaga uuucugaacu cgacuauaaa aacaagcgca aauucuuuga gcccuucagc     2280

ggauuccggc agaagguccu ggauaagauc gacgagaucu uuguguccaa accugaaaga     2340

aagaaacccu cuggcgcgcu gcaugaggaa accuuccgga aggaggaaga guuuuaccaa     2400

agcuacgggg gaaaagaggg cguucucaag gcccuggaac uuggcaaaau ucgcaaggug     2460

aaugggaaga ucgucaaaaa cggagauaug uucagagugg acauuuuuaa gcacaaaaag     2520

acaaauaaau ucuaugcugu cccgaucuac accauggauu uugcccugaa ggugcucccu     2580

aacaaggccg uugcucgguc caaaaagggc gagauuaaag acuggauccu gauggaugaa     2640

aauuaugagu ucugcuuuag ccuguacaag gacucucuua uccugauuca gacaaaagac     2700

augcaggaac ccgaguucgu guauuacaac gccuuuaccu ccagcacggu cucucucauc     2760

guguccaagc augauaauaa guucgagaca cugagcaaaa accagaagau ucucuucaaa     2820

aaugcgaacg aaaaggaggu uaucgcuaaa ucuauuggga uccaaaaucu gaagguguuu     2880

gaaaaguaua ucgucagcgc ccuuggagag gugaccaaag ccgaguucag acagcgcgaa     2940

gacuuuaaga aauga                                                      2955


<210>  3723
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3723
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcucgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaaacuugc gaacuaucag      480

agcguugggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcgucgcu uuuuauaaga gagcccugaa ggauuucucu      720

caucuugugg gcaacugcuc cuuuuucacc gacgagaaac gggcucccaa gaauagcccu      780

cuggccuuua uguucguugc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugcuc ugcucaacga guuucugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uucaagggcg aaaaagggac guauuuuauu gaguucaaga aauacaagga auuuaucaag     1020

gcgcuuggag agcacaaccu gucucaggac gaucucaaug aaauugccaa agacaucacc     1080

cugauuaagg augagaucaa acugaagaaa gcucucgcca aguaugaccu gaaccaaaau     1140

cagaucgaua gccuuuccaa acuggaguuc aaggaccauc ucaacauuag cuuuaaggcc     1200

cugaaacuga ucacaccccu uaugcucgaa ggcaagaaau acgaugaggc uuguaaugaa     1260

cugaaccuga agauugccau caaugaggac aaaaaggauu uccucccugc guucaacgag     1320

accuacuaua aggacgaagu gacaaauccc gucgugcugc gcgcuauuaa agaguaccgg     1380

aagguucuua acgcccugcu caaaaaguau gggaaagugc acaagaucaa uaucgaacug     1440

gccagagagg ucggaaagaa ccacucucag cgcgcuaaaa uugaaaagga gcagaacgag     1500

aauuacaaag ccaagaaaga cgcugaacug gagugcgaaa aacuuggccu caagaucaac     1560

uccaagaaua uucugaagcu gcggcucuuu aaagagcaaa aggaguucug ugccuauagc     1620

ggcgagaaaa ucaagauuuc ugaucugcag gacgagaaaa ugcuugaaau cgaucauauu     1680

uacccuuaua gcagauccuu ugacgauucu uacaugaaca aggugcuggu guucaccaag     1740

cagaaucaag agaaacucaa ccagacgccg uuugaagcgu ucgggaauga cagcgccaag     1800

uggcagaaaa ucgagguccu ggcuaagaac cugcccacaa agaagcagaa gcgcauccuu     1860

gauaaaaauu acaaggacaa agaacaaaag aacuuuaaag aucggaaucu caacgacacc     1920

agauauauug cccgccuggu ucugaauuac acaaaggauu aucucgacuu ccugccucuu     1980

uccgaugacg agaacaccaa gcugaaugac acccagaaag gaagcaaggu gcacguggag     2040

gcuaaaucug gcaugcucac auccgcccug cggcauacgu ggggguuuag cgcgaaggau     2100

agaaacaacc aucugcacca cgccaucgac gcugucauua ucgccuacgc uaauaacucu     2160

auugugaaag ccuucuccga uuuuaagaag gaacaggaga gcaauuccgc cgaacuuuau     2220

gcuaaaaaga ucucugagcu cgacuacaaa aacaagcgca aauucuuuga gcccuucagc     2280

ggauuccggc agaagguucu ggauaagauc gacgaaauuu uuguguccaa accugagaga     2340

aagaaacccu cuggcgcgcu gcacgaagag accuuccgga aggaagagga guuuuaucaa     2400

agcuacgggg gaaaagaagg cguccucaag gcccuggagc uuggcaaaau ccgcaaggug     2460

aaugggaaga uuguuaaaaa cggagauaug uucagagugg acaucuuuaa gcauaaaaag     2520

acaaauaaau ucuacgcugu cccgauuuau accauggauu uugcccugaa ggugcucccu     2580

aacaaggccg ucgcucgguc caaaaagggc gaaaucaaag acuggauccu gauggaugag     2640

aauuacgagu ucugcuuuag ccuguauaag gacucucuua uucugaucca gacaaaagau     2700

augcaggaac ccgaguucgu guacuauaac gccuuuaccu ccagcacggu uucucucauu     2760

guguccaagc acgacaauaa guucgaaaca cugagcaaaa accagaagau ccucuucaaa     2820

aaugcgaacg agaaggaagu cauugcuaaa ucuaucggga uccaaaaucu gaagguguuu     2880

gagaaguaca uuguuagcgc ccuuggagaa gugaccaaag ccgaguucag acagcgcgag     2940

gacuuuaaga aauga                                                      2955


<210>  3724
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3724
auggugagaa uccuggccuu cgacauuggc aucagcucca uugggugggc uuuuucugag       60

aacgaugaac ucaaggacug cggaguccgg aucuucacca aaguugagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcgcgguccg cucgcaaaag acucgcccgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcuu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcgaga      360

gugauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaagcucgc uaacuaucag      480

agcguggggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ucagaaauaa aaaggagucc uacgaacggu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcguugcg uuuuauaaga gagcccugaa ggauuucucu      720

caucuugugg gcaacugcuc cuuuuucacc gacgagaaac gggcucccaa gaauagcccu      780

cuggccuuua uguucgucgc ucucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugccc ugcucaacga ggugcugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac guauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcccuuggag agcacaaccu gucucaggac gaucucaaug aaauugcuaa agacaucacc     1080

cugauuaagg augagaucaa acugaagaaa gcgcucgcca aguaugaccu gaaccaaaau     1140

cagaucgaua gccuuuccaa acuggaguuu aaggaccauc ucaacauuag cuucaaggcu     1200

cugaaacuga ucacaccccu uaugcucgaa ggcaagaaau acgaugaggc cuguaaugaa     1260

cugaaccuga agguugccau uaaugaggac aaaaaggauu uucucccugc uuucaacgag     1320

accuacuaua aggacgaagu gacaaauccc gucgugcugc gcgccaucaa agaguaccgg     1380

aaggugcuua acgcgcugcu caaaaaguau gggaaagucc acaagauuaa uaucgaacug     1440

gcuagagagg uuggaaagaa ccacucucag cgcgccaaaa ucgaaaagga gcagaacgag     1500

aauuacaaag ccaagaaaga cgcugaacug gagugcgaaa aacuuggccu caagauuaac     1560

uccaaaaaua uccugaagcu gcggcuuuuc aaagagcaaa aggaauuuug ugccuauagc     1620

ggcgagaaaa uuaagaucuc ugaucugcag gacgagaaaa ugcuugaaau ugaucauauc     1680

uacccuuaua gcagauccuu cgacgauucu uacaugaaca aggugcuggu guuuaccaag     1740

cagaaucaag agaaacucaa caagacgccg uucgaggcuu uugggaauga cagcgccaaa     1800

uggcagaaga uugaaguccu ggcgaaaaac cugcccacaa agaagcagaa acgcauccuu     1860

gauaagaauu acaaagacaa ggaacagaaa gauuucaagg accggaaccu caaugauacc     1920

agauauaucg cccgccuggu gcugaacuac acaaaggacu aucucgauuu ucugccucuu     1980

uccgacgacg agaauaccaa acugaacgau acccaaaagg gaagcaaagu ucacguggag     2040

gcuaagucug gcaugcucac auccgcccug cggcauacgu ggggguucag caccaaagac     2100

agaaauaacc aucugcacca cgcuauugau gccgucauca uugcguacgc caacaauucu     2160

aucgugaagg cuuuuuccga cuucaagaaa gaacaggaga gcaacuccgc cgaacuuuau     2220

gcuaagaaaa uuucugagcu cgauuacaag aauaaacgca aguuuuucga gcccuuuagc     2280

ggauuccggc agaagguucu ggacaaaauc gaugaaaucu ucguguccaa gccugagaga     2340

aagaagcccu cuggcgcccu gcacgaagag acauuucgga aagaagagga guucuaucag     2400

agcuacgggg gaaaggaagg cguccucaaa gcccuggagc uuggcaagau ucgcaaggug     2460

aacgggaaaa ucgucaagaa uggagacaug uuuagagugg auauuuucaa acauaagaaa     2520

accaacaagu uuuacgcugu uccgaucuau acaauggacu ucgcgcugaa ggugcucccu     2580

aauaaagccg ucgcucgguc caagaaaggc gaaauuaagg auuggauccu gauggacgag     2640

aacuacgaau uuugcuucag ccuguauaaa gauucucuua uccugauuca aaccaaggac     2700

augcaggagc ccgaguuugu guacuauaau gccuucacgu ccagcacagu uucucucauc     2760

guguccaagc acgacaacaa auuugaaacc cugagcaaga aucagaaaau ucucuucaag     2820

aacgccaaug agaaagaagu caucgcuaag ucuauuggga uccagaaccu gaagguguuc     2880

gagaaauaca ucgugagcgc ccuuggagaa guuaccaagg cggaguuuag acaacgcgag     2940

gauuucaaaa aguga                                                      2955


<210>  3725
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3725
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcucgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaaacuugc gaacuaucag      480

agcguugggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcgucgcu uuuuauaaga gagcccugaa ggauuucucu      720

caucuugugg gcaacugcuc cuuuuucacc gacgagaaac gggcucccaa gaauagcccu      780

cuggccuuua uguucguugc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugcuc ugcucaacga ggugcugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac guauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcgcuuggag accacucucu gagccaggau gaccucaacg agauugccaa agauaucacc     1080

cugauuaagg acgaaaucaa acugaagaaa gcucucgcca aguaugaucu gaaucaaaac     1140

cagaucgacu cccuuagcaa acuggaguuu aaggaucauc ucaauauuuc uuucaaggcc     1200

cugaaacuga ucacaccccu uaugcucgag ggcaagaaau acgacgaggc uuguaacgaa     1260

cugaaucuga aggucgccau uaacgaagau aaaaaggacu uucucccugc guucaaugag     1320

accuacuaua aggacgaggu gacaaacccc guugugcugc gcgcuaucaa agaauaccgg     1380

aagguccuua augcccugcu caaaaaguau gggaaagugc acaagauuaa caucgagcug     1440

gccagagaag ugggaaagaa uuacucccag cgcgcuaaaa ucgagaagga acagaacgag     1500

aacuauaaag ccaagaaaga ugcugagcug gaaugcgaga aacuuggccu caagauuaau     1560

agcaaaaaca uccugaagcu gagacucuuc aaagaacaaa aggaguuuug ugccuacucu     1620

ggcgaaaaga uuaagaucag cgaccugcag gaugagaaaa ugcuugagau ugaccacauc     1680

uauccuuacu cccggucuuu cgaugacagc uacaugaaua agguccuggu uuuuaccaag     1740

cagaaccaag aaaaacucaa ucagacgccg uucgaggcgu uugggaacga uuccgccaag     1800

uggcagaaaa uugaagugcu ggcuaagaau cugcccacaa agaagcagaa gcgcauccuu     1860

gacaaaaacu auaaggauaa agagcaaaag gacuucaaag aucggaaucu caacgacacc     1920

agauacaucg cccgccuggu gcugaauuau acaaaggauu accucgacuu ucugccucuu     1980

agcgacgaug aaaacaccaa gcugaaugac acccagaaag gaucuaaggu ccauguggag     2040

gcuaaauccg gcaugcucac aagcgcccug cggcacacgu ggggguucuc ugcgaaggau     2100

agaaacaauc aucugcacca cgccauugac gcuguuauca uugccuaugc uaacaacucc     2160

aucgugaaag ccuuuagcga uuucaagaag gagcaggaau ccaauucugc cgagcuuuac     2220

gcuaaaaaga uuagcgaacu cgacuauaaa aacaagcgca aauuuuucga gcccuuuucc     2280

ggauuccggc agaagguccu ggauaagauc gacgagaucu ucgugucuaa accugaaaga     2340

aagaaaccca gcggcgcgcu gcaugaggaa accuuucgga aggaggaaga guucuaccaa     2400

uccuacgggg gaaaagaggg cguucucaag gcccuggaac uuggcaaaau ucgcaaggug     2460

aaugggaaga ucgucaaaaa cggagauaug uuuagagugg acauuuucaa gcacaaaaag     2520

acaaauaaau uuuaugcugu cccgaucuac accauggauu ucgcccugaa ggugcucccu     2580

aacaaggccg uugcucggag caaaaagggc gagauuaaag acuggauccu gauggaugaa     2640

aauuaugagu uuugcuucuc ucuguacaag gacucccuua uccugauuca gacaaaagac     2700

augcaggaac ccgaguuugu guauuacaac gccuucacca gcucuacggu cucccucauc     2760

gugagcaagc augauaauaa guuugagaca cugucuaaaa accagaagau ucucuucaaa     2820

aaugcgaacg aaaaggaggu uaucgcuaaa agcauuggga uccaaaaucu gaagguguuc     2880

gaaaaguaua ucgucuccgc ccuuggagag gugaccaaag ccgaauuuag acagcgcgag     2940

gacuucaaga aauga                                                      2955


<210>  3726
<211>  2958
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3726
auggccaaga uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuguggag       60

aacgaugaac ucaaagacug cggagucaga aucuucacca aggcggagaa ucccaaaaca      120

ggcgaaucuc uggcgcuucc ucggcgccug gccagaagcg cccggaagcg ccucgcuaga      180

cggaaagccc gccugaacca ccuuaagcau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc aguccuucga cgagucucug gccaaagcgu acaaggggac gcugaucagc      300

ccguaugagc ucagauuuaa agcccugaau gaacuucugu ccaagcaaga ucucgcucgg      360

guuauccugc acauugccaa acgcagagga uacgacaaca ucaaaaauuc ugaugacaag      420

gagaagggcg cuauucuuaa ggccgugaaa gaaaacgagg agaagcuggc gaauuauaaa      480

agcguggggg aauaccucua uaaggaguac uuccagaaau uuaaggaaaa cuccaaggag      540

uucaucaaug uccggaacaa aaaggaaagc uacgagcgcu guauugccca gucuuuucug      600

aaagaugagc ugaagcucau cuuccagaaa caaagagaau uuggauucaa uuuuuccaag      660

aaguucgagg aagaggugcu gagcguugcu uucuauaaac gggcccuuaa ggacuuuucu      720

caucuggugg gcaacugcuc cuucuuuguc gaugaaaaac gcgcucccaa gaauagcccu      780

cucgccuucg aguuuguggc ccugaccaga auuguuaacc uucugaacaa ucucaacaaa      840

aauaacucuu acaucaagga cgaucugaau gcucugcucu ccgaggugcu gaagaacggc      900

acacuuaccu auaaacagac aaagaaaauc cuggggcuca gcgacgacua cgaauuuaag      960

ggacagaaag gcaauuauuu cauugaguuc aagaaguaca aagaauuuau caaggcgcug     1020

ugugagcaca accuuaauca ggaugaccug aacgaaauug ccaaagauau cacccucguc     1080

aaggacgaga caaaacugaa gaaagcucug gccaaguaug aucucaauca aaaccagauu     1140

gagaaucugu ccaagaucga guucaaagac caucuuaaca ucucuuuuaa ggcccugaaa     1200

cucauugugc cccugaugcg ggaagggaag aaauacgaug aagugugcaa ugagcugaac     1260

cuuaagaccg cuaucaacga ggacaagaaa gaguuccucc cugccuuuaa ugaaacguac     1320

uauaaggaug aagucaacaa ucccguugug cugagagcga uuaaagagua ccgcaaggug     1380

cugaacagcc uccugaaaaa guauggaaag guccacaaaa ucaauauuga acuugcucgg     1440

gaggugggca agaaccacag agagcgcucc aaaaucgaaa aggagcagaa ugaaaacuuc     1500

aaagccaaga aggacgccga gcuggaaugu gagaaacucg ggcugaagau caauagcaaa     1560

aacauucuga agcuccggcu uuucaaagag cagaaggaau uuugcguuua cucuggagag     1620

aaaaucaaga uuuccgaucu gcaagacgaa aagaugcugg agaucgacca uauuuauccu     1680

uacagcagau cuuucgauga cagcuauaug aauaaagugc ucgucuuuac caagcagaac     1740

caggaaaaac ugaauaagac accguucgag gcuuuuggca aagaugacgu gaaguggcaa     1800

aagaucgaga cccuugccaa aaaccugccc gcuaagaaac agaagcgcau ucucgauaaa     1860

aauuacaagg acaaggaaca gaaagauuuc aaggaccgga accugaacga uacaagauac     1920

aucgcccgcc ugguucuuaa uuauaccaaa gacuaccucg aguuucugcc ucuguccgau     1980

gacgaaaaca ucaagcucaa ugauacgcag aaaggcucua aggugcacgu cgaggcuaag     2040

agcgggaugc ugacauccgu gcuucggcau accuggggau ucagcacaaa agacagaaac     2100

aaucaucugc accacgccau ugacgcuguc aucauugccu augcuaacgc cucugugguu     2160

aaggcguuuu ccgauuucaa aaaggagcaa gaaaauuaca ccgccgagcu cuaugcuaaa     2220

aagaucaacg aacuggacuu uaagaauaaa cgcaaguucu uugagcccuu cagcaacuuc     2280

cggcagaaag ugcuggaaaa gauugaugag aucuuugucu cuaaaccuga gagaaagaaa     2340

cccuccggcg cccuucacga agagaccuuc uacaaggaag aggaauuuua uaaggaguac     2400

gggggaaaag agggcgugcu caaggcucug gaacugggga aaauccggaa gguuaaugga     2460

aaaauuguga gcaacgccaa uauguuccgc gucgacaucu uuaaguacaa gaaaacaaac     2520

aaguucuaug ccgugccgau uuacacgaug gauuuugcuc ucggcauccu gccuaauaaa     2580

gcggugguug gcgggaagga caaacaggga auuaucaagg auuggcuucu gauggacgag     2640

aacuaugagu ucuguuuuuc ccucuacaag gauucucuga uccugcuuca gaccaaagac     2700

augcaagaac ccgaacuggu cuauuucaag agcuuuaaau ccucuacagc cggccucgug     2760

gugagcaagc augauaacaa auucgagaau cugaacgaga aucagaagaa acucuucaag     2820

uccgcuaacg aaaagaaagu cguggccgag gggauuggaa uccagaaucu gaagguuuuu     2880

gaaaaauaca uugugagcgc ccuuggcgag aucaccaagg cugagauuaa agaaagagag     2940

aacuucaaga agaaauga                                                   2958


<210>  3727
<211>  2961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3727
augcccagcg acccugccga ggcuaagccg cuggaacucc uggaucccua cgcccuuaga       60

gcgcagggcc uggaccaagc ucucccucug acccacuucg ggcgggcccu uuuucaucug      120

aaccagcgca gaggauucaa auccaaucgg aagacagauc gcggcgacaa cgagaauggg      180

aaaaucaagg augccucuca gagacucgac ugggcuaugc acgaagccaa agcucggacg      240

uauggagagu uucugcaucu gcgcagacag aaggcccccg auccucggca agugcccacc      300

guccgcacaa gacucagcgu uggcaagcgc gggggaccug acgcgaaaga agaggccggc      360

uacgauuucu auccggaccg gagacaccug gaggaagagu uucaugaacu uugguccgcu      420

caggcccagu uccaccccga gcugacccag gaucuccggg accugguguu ugagaagauu      480

uucuaccaac gcccucuuaa agaacccaag gugggccugu gccucuuuac aucugaggaa      540

agacuggcua aagcccaccc ucugacccag cggagagucc ucuaugagac agugaaccag      600

cugcgcauca ccgcggaugg gcggagcguu agaccccuuu cccgcgaaca gcgggaccaa      660

auuauccugg cccucgacac gaagaagccg accaaaucuc ugaauagcau gguggucaca      720

cuucaggcuc ugggaaaggu gcucaaacug agagagggcg agcgcuucac ccuggaaucc      780

gggguucggg aggccauugc uugugauccu gugagagccg ccaugcucgc uacauuugga      840

ccccgcugga gcgcgcugga cuggccugaa caguggcaag ucaucucucg ggugagaaaa      900

cuucaggaug acaaggauuu cgccgagcug guggaauggc ucaucgagcu guacggcguc      960

gacaaggagg cugccguuaa aauugccaac acccaucuuc ccgaagggua uggacgccug     1020

ggccucacgg cuacaacccg gauccuggau agacugaagu ccaauguguu uacauacgcc     1080

caggcggugg cugccugcgg cuggcaccau agcgaccagc ggaccgggga gugucucgau     1140

cgccugcccu acuauggagc cguccuugac agacacguga uuccuggcac cuacgaugaa     1200

aacgacgacg auaucacacg guucgggaga auuacgaauc cgaccguuca caucggacug     1260

aaccaacucc gccggcuggu gaauaagauu aucgaggucu auggcaaacc caaccagauc     1320

gugguugaac uggcuagaga gcuuaagcag ucugagcagc agaaaaauga cgccauuaag     1380

cucauccgcg auacaaccac agcugccaaa cagagauccg aaaagcugga ggaacugggg     1440

auuaccgaca acggacggaa ucgcaugcuc cugagacuuu gggaggaucu gaacccugac     1500

gaugcgaugc ggcgcuucug ccccuacacg ggccagagaa ucagcgccgc uaugauuuuu     1560

gacggcucuu gugaugugga ccauauccuc ccuuauuccc ggacacugga ugacggguuc     1620

gccaaccgca cccugugccu uagagaggcu aaucgggaaa agcgcaacca gacccccugg     1680

gaggccuggg gaggcacacc gaauugggaa gcgaucgaga ccaaccucaa aaaucugccu     1740

gaaaacaaga gauggcgguu ugcccccgau gcuauggcca gauucgaggg ggagaaugac     1800

uuucuggacc gcgcucucgu cgauacacaa uaccuggccc ggauuagcag aacguaucuu     1860

gacacccugu ucacagaagg aggccacgug ugggucgugc cugggcgccu caccgagaug     1920

cugcggagac auuggggccu gaacucccuu cucacagaua aagaccgcgg agccguuaag     1980

ucuaaaaauc ggaccgauca cagacaucac agcaucgacg cugcgguggu cgccgcuacg     2040

gaucgcuccc ugcugaaccg gauuagcaga gccgccggcc agggggaagu ggcuggacag     2100

ucugccgagc ucaucgcgcg ggaaacaccc ccucccuggg agggcuuucg caaugaccug     2160

aagucccagc uugggagaau uaucguuagc caccgggcug aucauggacg caucgacaga     2220

caagcccgga agcagggcag agauucuaca gccgggcagc ugcaccaaga gaccgcuuac     2280

agcauugugg acgauacacg cgucgccucc cggaucgacu uccucucugu gaaaccggcg     2340

cagcugcugg accagccugg cagaagcgga caggugcgcg aucuucaacu ccggaaggcu     2400

cugagaguug ccaccggcga ccugacgggg aaagauuacg aaaaggcccu ccgcgacuuu     2460

gcugccaaac ccggacccua uaagggcauu cggagagucc gcaucauuaa gccucugcag     2520

gagcaggcua gagugccggu gcccguccuu gauccuauca aagccuacca agggggaucc     2580

aaccaugugu ucgaaauuug gcaucugccc gacggcaagg uugagccuca agugaucacc     2640

agcuuugaag cgcacgcccu cgaggucgag aaacggcccc acccugcugc caagcgccug     2700

cugagagugc acaaagggga uaugguugcu cuuaagcggg aaggccgcac cguggucgga     2760

caugugcagc agaugcacau caaaaauggc cuguucauug uuccccauaa cgaggccaau     2820

gccgacgcua gaaacucuga ucggacagac ccguuuaagu ggauccagau ugcggcccaa     2880

ccugcuaucg ccgccgggau uagacgcgug gcugucgaug aaaucggacg gcucagagac     2940

ggcggggcca agaccaucug a                                               2961


<210>  3728
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3728
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug ucccggucug cucgcaaaag acucgcccgg      180

cgcaaggcua gacugaacca ccuuaaacau cugauugcca augaguuuaa gcucaacuac      240

gaagauuauc agagcuucga cgagucccug gcuaaggccu acaaaggguc ucugaucagc      300

ccguaugagc uccgguuucg cgcgcugaau gaguuccuuu ccaagcaaga uuuugccaga      360

gucauccugc acauugcuaa acggcgcgga uacgacgaua ucaagaacaa uggcgacgag      420

gaaaaagggg agauucucaa ggccaucaag cagaacgagg aaaaacuggu uagcuaucag      480

ucugugggag aguaccuuua uaaggaauac uuccagaaau uuaaggaaaa uuccaaagag      540

uucauuaacg ugagaaauaa gaaagaaagc uacgagcggu guaucgcuca aucuuuucug      600

aaggaugaac ucaagcugau uuuccagaaa cagcgcgagu uuggcuucuc cuucagcaag      660

aaauuugaag aggaaguccu gucuguggcc uucuauaaga gagcgcucaa agacuuuucc      720

caucugguug gcaacugcag cuucuuuacg gaugagaagc gggcccccaa aaauuccccu      780

cuugcuuuca uguuuguggc ccugaccaga aucaucaacc uccugaauaa ccuuaagaac      840

acagagggga uucuguacac caaggacgau cucaauacac ugcugaacga aguccucaaa      900

aauggaaccc ugacauauaa gcagaccaaa aagcugcuug gccucucuaa cgacuacgag      960

uucaaggggg aaaaaggaac guauuuuauc gaguuccaaa aguacaaaga auuugugaag     1020

gcucugggcg agcacaaucu uagccaggac gaucugaacg agauugccaa agacaucacc     1080

cucauuaagg augaaaucaa gcugaaaaag gcccuggcua aauaugaccu caaucagaac     1140

cagaucgauu cccugagcaa acuugaguuc aaggaccauc ugaauauuuc uuuuaaggcg     1200

cucaaacuga ucacaccccu gaugcuugaa gggaagaagu acgaugaggc cuguaacgag     1260

cucaaucuga aaguugcuau uaacgaagac aagaaagauu uccugccugc cuucaaugag     1320

accuacuaua aggacgaagu gacaaacccc gucgugcucc gcgccaucaa agaguaccgg     1380

aaggugcuga augcccuucu gaagaaauau ggaaaggucc acaaaauuaa caucgaacuc     1440

gcuagagagg uuggcaagaa ccacucccaa cgcgcgaaaa ucgagaagga acagaaugag     1500

aacuacaagg cuaaaaagga ugccgaacug gagugcgaaa aacugggccu uaagauuaau     1560

agcaaaaaca uccucaagcu gcggcuguuu aaggagcaga aagaguucug uguguauucu     1620

ggggaaaaga uuaaaaucag cgaccuccaa gacgagaaga ugcuggaaau ugaucauauc     1680

uacccuuauu ccagaucuuu ugacgauagc uacaugaaua aagugcuugu cuucaccaag     1740

cagaaccagg agaaacugaa uaagacgccg uuugaaacau ucggaaacga ccgcgugaag     1800

uggcagaaaa uugagguucu cgccaagaau cugcccacca aaaagcaaaa acggauccug     1860

gauaagaacu acaaggacaa agagcagaag gauuuuaaag acagaaaucu uaacgauaca     1920

cgcuauaucg cucggcucgu gcugaauuac accaaggacu aucuggauuu ccucccucug     1980

uccgacgaug aaaacaccaa acuuaaugac acacagaagg gcagcaaggu ccacguggag     2040

gccaaaucug ggaugcugac guccacccuc agacauacau ggggauuuag caccaaggac     2100

cgcgauaacc aucugcacca cgcuauugac gccgcgauca uugccuacgc uaacaauucu     2160

aucguuaaag ccuucuccga uuuuaagaaa gaacaggaga gcaacacagc ugagcuguau     2220

gccaagaaga uuuccgaacu ugacuacaaa aauaagcgga aauucuuuga gcccuucucu     2280

ggcuucagac aaaagcuccu ggauaaaauc gacgaaaucu uugugagcaa gccugagcgg     2340

aagaaacccu ccggggcgcu gcacgaagag accuuccgca aggaggaaga guuuuaucag     2400

ucuuacggag gcaaagaagg cguccucaag gcccuggagc uugggaaaau uagaaaggug     2460

aacggaaaaa uggucaagaa uggcgauaug uuccgggugg acaucuuuaa gcauaaaaag     2520

acgaacaaau ucuacgcugu uccgauuuau acaauggauu uugcccugaa ggugcucccu     2580

aauaaagcug ucgugcaggg gaaggacaag aaaagcggac ugaucaagga uuggauucug     2640

auggacgaaa acuacgaguu cugcuuuucc cuuuauaaag auagccugau ccucauccag     2700

accaaggaca ugcaagagcc cgaacugguu uacuaugaga gacucgacuc uuccaaugcc     2760

agccugaccg ugucuaaaca cgauaacaag uucgaaacac uuuccaagaa ucagaaaauu     2820

cuguuuaaga acgccaauga gaaagaaguc aucgcuaaga gcauuggcau ccagaaccuc     2880

aaaguguucg agaaguacau ugugucugcg cugggggagg uuaccaaggc cgaguuccgc     2940

cagcgggaag acuuuaaaaa guga                                            2964


<210>  3729
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3729
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcucgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaaacuugc gaacuaucag      480

agcguugggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcgucgcu uuuuauaaga gagcccugaa ggauuucucu      720

caucuugugg gcaacugcuc cuuuuucacc gacgagaaac gggcucccaa gaauagcccu      780

cuggccuuua uguucguugc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugcuc ugcucaacga ggugcugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac guauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcgcuuggag accacucucu gagccaggau gaccucaacg agauugccaa agauaucacc     1080

cugauuaagg acgaaaucaa acugaagaaa gcucucgcca aguaugaucu gaaucaaaac     1140

cagaucgacu cccuuagcaa acuggaguuu aaggaucauc ucaauauuuc uuucaaggcc     1200

cugaaacuga ucacaccccu uaugcucgag ggcaagaaau acgacgaggc uuguaacgaa     1260

cugaaucuga aggucgccau uaacgaagau aaaaaggacu uucucccugc guucaaugag     1320

accuacuaua aggacgaggu gacaaacccc guugugcugc gcgcuaucaa agaauaccgg     1380

aagguccuua augcccugcu caaaaaguau gggaaagugc acaagauuaa caucgagcug     1440

gccagagaag ugggaaagaa uuacucccag cgcgcuaaaa ucgagaagga acagaacgag     1500

aacuauaaag ccaagaaaga ugcugagcug gaaugcgaga aacuuggccu caagauuaau     1560

agcaaaaaca uccugaagcu gagacucuuc aaagaacaaa aggaguuuug ugccuacucu     1620

ggcgaaaaga uuaagaucag cgaccugcag gaugagaaaa ugcuugagau ugaccacauc     1680

uauccuuacu cccggucuuu cgaugacagc uacaugaaua agguccuggu uuuuaccaag     1740

cagaaccaag aaaaacucaa ucagacgccg uucgaggcgu uugggaacga uuccgccaag     1800

uggcagaaaa uugaagugcu ggcuaagaau cugcccacaa agaagcagaa gcgcauccuu     1860

gacaaaaacu auaaggauaa agagcaaaag gacuucaaag aucggaaucu caacgacacc     1920

agauacaucg cccgccuggu gcugaauuau acaaaggauu accucgacuu ucugccucuu     1980

agcgacgaug aaaacaccaa gcugaaugac acccagaaag gaucuaaggu ccauguggag     2040

gcuaaauccg gcaugcucac aagcgcccug cggcacacgu ggggguucuc ugcgaaggau     2100

agaaacaauc aucugcacca cgccauugac gcuguuauca uugccuaugc uaacaacucc     2160

aucgugaaag ccuuuagcga uuucaagaag gagcaggaau ccaauucugc cgagcuuuac     2220

gcuaaaaaga uuagcgaacu cgacuauaaa aacaagcgca aauuuuucga gcccuuuucc     2280

ggauuccggc agaagguccu ggauaagauc gacgagaucu ucgugucuaa accugaaaga     2340

aagaaaccca gcggcgcgcu gcaugaggaa accuuucgga aggaggaaga guucuaccaa     2400

uccuacgggg gaaaagaggg cguucucaag gcccuggaac uuggcaaaau ucgcaaggug     2460

aaugggaaga ucgucaaaaa cggagauaug uuuagagugg acauuuucaa gcacaaaaag     2520

acaaauaaau uuuaugcugu cccgaucuac accauggauu ucgcccugaa ggugcucccu     2580

aacaaggccg uugcucggag caaaaagggc gagauuaaag acuggauccu gauggaugaa     2640

aauuaugagu uuugcuucuc ucuguacaag gacucccuua uccugauuca gacaaaagac     2700

augcaggaac ccgagcucgu guauuuuaac gccuucacca gcucuacggu cucccugauc     2760

gugagcaagc augauaauaa guuugagaca cucucuaaaa accagaagau ucuguucaaa     2820

aaugcgaacg aaaaggaggu uaucgcuaaa agcauuggga uccaaaaucu uaagguguuc     2880

gaaaaguaca ucgucuccgc ccugggagag gugaccaaag ccgaauuuag acagcgcgag     2940

gacuucaaga acgagcuccg guga                                            2964


<210>  3730
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3730
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcucuccc ucgcagacug gcccgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacaa uggcgacgag      420

gaaaaauccg agauucugaa ggcuaucaag cagaacgagg aaaaacuugu uaauuaucag      480

agcguggggg aguaccugua uaaggaauac uuucagaaau ucaaggagaa cucuaaagaa      540

uuuauuaaug ugagaaacaa gaaagagucc uacgagcggu guaucgccca gagcuuccuc      600

aaggaugaac ugaagcugau uuuucaaaaa cagcgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaaguccu cagcguggcg uuuuauaaga gagcccugaa agacuucucu      720

caucuuguug gcaauugcuc cuuuuucacg gaugagaagc gggcucccaa gaacagcccu      780

cugguguuua uguucgucgc ccucaccaga aucaucaauc ugcuuaacaa ccugaaaaau      840

acagagggca uucucuacac caaggacgau cugaacgcuc ugcucaauga agugcugaaa      900

aacgggacac uuaccuauaa gcagaccaaa aagcugcucg gacuguccga cgacuacgag      960

uuuaagggcg aaaaagggac cuauuucauc gaguuuaaga aauacaagga guucauuaaa     1020

gcccuugaug aacacucucu gagccaagac gaucucaaug agaucgccaa ggacauuacg     1080

cugaucaagg augaaaucaa acugaagaaa gcucucgcga aguaugaccu gaaagagaac     1140

cagauugauu cccuuagcaa gcuggaauuu aaagaccauc ucaauaucuc uuucaaggcc     1200

cugaagcuga uuacaccccu uaugcucgag ggcaaaaagu acgaugaggc uuguaacgaa     1260

cugaaucuga aaguugccau caacgaggac aagaaagauu uucucccugc cuucaaugaa     1320

acauacuaua aggacgaggu gaccaacccc gucgugcugc gcgcuauuaa ggaauaccgg     1380

aaaauccuua augcccugcu caagaaauau ggaaaggugc acaaaaucaa cauugagcug     1440

gcgagagagg ucgggaagaa ucacucccag cgcgcuaaga ucgaaaaaga gcagaacgaa     1500

aacuacaagg ccaaaaagga cgccgagcug gaaugcgaga aacuuggacu caagauuaau     1560

agcaagaaca uccugaaacu gcggcuuuuc aaggagcaaa aagaauuuug uguuuauucu     1620

ggcgagaaga uuaaacugag cgaucuucag gacgaaaaga ugcuggagau cgaucauauu     1680

uacccuuauu ccagaucuuu cgacgauagc uacaugaaua aagugcucgu guuugcuaag     1740

cagaaccaag aaaagcugaa uaaaacaccg uucgaggccu uuggcaacga cuccaccaag     1800

uggcagaaaa ucgagguccu gacgaagaau cuucccacca aaaagcagaa gcgcauccuc     1860

gauaaaaacu acaaggacaa agaacagaag gauuucaaag accggaaucu gaacgauaca     1920

agauauauug cucgccuggu gcucaauuac acaaaggacu aucuggauuu ucuuccucug     1980

agcgacgacg agaacaccaa gcucaaugau acccaaaaag ggucuaaggu ucacguggaa     2040

gccaaauccg gaaugcugac aagcgcgcug cggcauacgu ggggcuucuc uaccaaggac     2100

agaaacaauc accuucacca ugccaucgau gcugucauua ucgccuacgc uaacaacucc     2160

auugugaaag ccuuuagcga cuucaagaag gagcaggagu ccaauucugc ggaacucuau     2220

gccaaaaaga ucagcgagcu ggauuacaaa aacaagcgca aauuuuucga acccuuuucc     2280

ggguuccggc agaagguucu ggacaagauc gaugagauuu ucgugucuaa accugaaaga     2340

aagaaaccca gcggagcucu ccacgaggag acauuucgga aggaagagga guucuaucag     2400

uccuacggcg ggaaagaagg aguccugaag gcccuugaac ugggcaaaau ccgcaaggug     2460

aauggcaaga uugucaaaaa cggggacaug uuuagagugg auaucuucaa gcauaaaaag     2520

accaauaaau uuuacgcugu uccgauuuau acaauggacu ucgcccucaa ggugcugccu     2580

aacaaggccg ucgcucggag caaaaaggga gagaucaaag auuggauccu gauggacgag     2640

aauuacgaau uuugcuucuc ucuuuauaag gauucccuga uucucaucca aaccaaagac     2700

augcaggagc ccgaacuggu guacuuuaac gcguucacga gcucuacagu uucccucauu     2760

gugagcaagc acgacaauaa guuugagacc cugucuaaaa accagaagau ccuuuucaaa     2820

aaugccaacg aaaaggaggu cauugcuaaa agcaucggca uccagaaucu gaagguguuc     2880

gagaaguaua uuguguccgc ccucggggaa guuaccaaag ccgaguuuag acaacgcgaa     2940

gauuucaaga acgagcugcg guga                                            2964


<210>  3731
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3731
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcucgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaaacuugc gaacuaucag      480

agcguugggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcgucgcu uuuuauaaga gagcccugaa ggauuucucu      720

caucuugugg gcaacugcuc cuuuuucacc gacgagaaac gggcucccaa gaauagcccu      780

cuggccuuua uguucguugc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaauaccc ugcucaacga ggugcugaaa      900

aaugggacac uuaccuauaa gcagacgaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac cuauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcucuuggag agcacaaccu gucucaggac gaucucaaug aaauugcgaa agacaucaca     1080

cugauuaagg augagaucaa acugaagaaa gcccucgcua aguaugaccu gaaccaaaau     1140

cagaucgaua gccuuuccaa acuggaguuu aaggaccauc ucaacauuag cuucaaggcc     1200

cugaaacugg ucacaccccu uaugcucgaa ggcaagaaau acgaugaggc cuguaaugaa     1260

cugaaccuga agguggcuau caaugaggac aaaaaggauu uucucccugc cuucaacgag     1320

accuacuaua aggacgaagu uaccaauccc gugguccugc gcgcgauuaa agaguaccgg     1380

aaggugcuua acgcucugcu caaaaaguau gggaaagugc acaagaucaa uauugaacug     1440

gccagagagg ucggaaagaa ccacucucag cgcgccaaaa ucgaaaagga gcagaacgag     1500

aauuacaaag cuaagaaaga cgccgaacug gagugcgaaa aacuuggccu caagaucaac     1560

uccaagaaua uucugaagcu gagacucuuc aaagagcaaa aggaauuuug ugcuuauagc     1620

ggcgagaaaa ucaagauuuc ugaucugcag gacgagaaaa ugcuugaaau cgaucauauu     1680

uacccuuaua gccgguccuu cgacgauucu uacaugaaca agguucuggu guuuacgaag     1740

cagaaucaag agaaacucaa ccagacaccg uucgaagccu uugggaauga cagcgcgaag     1800

uggcagaaaa ucgaggugcu ggccaagaac cugcccacca aaaagcagaa gcgcauucuu     1860

gauaaaaauu acaaggacaa agaacaaaag aacuucaaag aucggaaucu caacgacaca     1920

agauauaucg cucgccuggu ccugaauuac accaaggauu aucucgacuu ucugccucuu     1980

uccgaugacg agaacaccaa gcugaaugac acacagaaag gaagcaaggu gcacguugag     2040

gccaaaucug gcaugcucac guccgcucug cggcauaccu ggggguucag cgccaaggau     2100

agaaacaacc aucugcacca cgcgaucgac gccgugauua ucgcuuacgc caauaacucu     2160

auugucaagg cuuuuuccga uuucaagaag gaacaggaga gcaauuccgc cgaacuuuau     2220

gccaaaaaga ucucugagcu cgacuacaaa aacaagcgca aauuuuucga gcccuuuagc     2280

ggauuccggc agaaggugcu ggauaagauu gacgaaaucu ucguuuccaa accugagaga     2340

aagaaacccu cuggcgcucu gcacgaagag acauuucgga aggaagagga guucuaucaa     2400

agcuacgggg gaaaagaagg cgugcucaag gcgcuggagc uuggcaaaau ccgcaagguc     2460

aaugggaaga uugugaaaaa cggagauaug uuuagagucg acaucuucaa gcauaaaaag     2520

accaauaaau uuuacgccgu gccgauuuau acaauggauu ucgcucugaa gguucucccu     2580

aacaaggccg uggcccgguc caaaaagggc gaaaucaaag acuggauucu gauggaugag     2640

aauuacgaau uuugcuucag ccuguauaag gacucucuua uccugaucca gaccaaagau     2700

augcaggagc ccgagcucgu cuacuuuaac gcuuucacgu ccagcacagu gucucugauu     2760

guuuccaagc acgacaauaa guuugaaacc cucagcaaaa accagaagau ccuguucaaa     2820

aaugccaacg agaaggaagu gauugcgaaa ucuaucggga uucaaaaucu uaaggucuuc     2880

gagaaguaua ucgugagcgc ucugggagaa gugaccaaag ccgaguuuag acagcgcgag     2940

gacuucaaga acgaacuccg guga                                            2964


<210>  3732
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3732
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcucgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaaacuugc gaacuaucag      480

agcguugggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcgucgcu uuuuauaaga gagcccugaa ggauuucucu      720

caucuugugg gcaacugcuc cuuuuucacc gacgagaaac gggcucccaa gaauagcccu      780

cuggccuuua uguucguugc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugcuc ugcucaacga ggugcugaaa      900

aauuccaccc uuacauauaa gcagaccaag aaacugcucg ggcugucuga cgauuacgag      960

uuuaagggag aaaaaggcac guauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcgcuugggg accacagccu gucccaggau gaccucaacg agauugccaa agauaucacc     1080

cugauuaagg acgaaaucaa acugaagaaa gcucucgcca aguaugaucu gaaucaaaac     1140

cagaucgaca gccuuucuaa acuggaguuu aaggaucauc ucaauauuuc cuucaaggcc     1200

cugaaacuga ucacaccccu uaugcucgag ggaaagaaau acgacgaggc uuguaacgaa     1260

cugaaucuga aggucgccau uaacgaagau aaaaaggacu uucucccugc guucaaugag     1320

accuacuaua aggacgaggu gacaaacccc guugugcugc gcgcuaucaa agaauaccgg     1380

aagguccuua augcccugcu caaaaaguau ggcaaagugc acaagauuaa caucgagcug     1440

gccagagaag uggggaagaa uuacagccag cgcgcuaaaa ucgagaagga acagaacgag     1500

aacuauaaag ccaagaaaga ugcugagcug gaaugcgaga aacuuggacu caagauuaau     1560

ucuaaaaaca uccugaagcu gagacucuuc aaagaacaaa aggaguuuug ugccuacagc     1620

ggcgaaaaga uuaagaucuc cgaccugcag gaugagaaaa ugcuugagau ugaccacauc     1680

uauccuuacu cucggagcuu cgaugacucc uacaugaaua agguccuggu uuuuaccaag     1740

cagaaccaag aaaaacucaa uaagacgccg uucgaggcgu uuggcaacga uagcgccaaa     1800

uggcagaaga uugaagugcu ggcuaaaaau cugcccacaa agaagcagaa acgcauccuu     1860

gacaagaacu auaaagauaa ggagcagaaa gacuucaagg aucggaaucu caacgacacc     1920

agauacaucg cccgccuggu gcugaauuau acaaaggauu accucgacuu ucugccucuu     1980

ucugacgaug aaaacaccaa acugaaugac acccaaaagg gguccaaagu ccauguggag     2040

gcuaagagcg gaaugcucac aucugcccug cggcacacgu ggggcuucuc cgcgaaagau     2100

agaaacaauc aucugcacca cgccauugac gcuguuauca uugccuaugc uaacaacagc     2160

aucgugaagg ccuuuuccga uuucaagaaa gagcaggaau cuaauagcgc cgagcuuuac     2220

gcuaagaaaa uuuccgaacu cgacuauaag aacaaacgca aguuuuucga gcccuuuucu     2280

ggguuccggc agaagguccu ggauaaaauc gacgagaucu ucgugagcaa gccugaaaga     2340

aagaagcccu ccggagcgcu gcaugaggaa accuuucgga aagaggaaga guucuaccag     2400

agcuacggcg ggaaggaggg aguucucaaa gcccuggaac uuggcaagau ucgcaaggug     2460

aauggcaaaa ucgucaagaa cggggauaug uuuagagugg acauuuucaa acacaagaaa     2520

acaaauaagu uuuaugcugu cccgaucuac accauggauu ucgcccugaa ggugcucccu     2580

aacaaagccg uugcucgguc uaagaaagga gagauuaagg acuggauccu gauggaugaa     2640

aauuaugagu uuugcuucuc ccuguacaaa gacagccuua uccugauuca aacaaaggac     2700

augcaggaac ccgagcucgu guauuuuaac gccuucaccu cuuccacggu cagccugauc     2760

gugucuaagc augauaauaa auuugagaca cucagcaaga accagaaaau ucuguucaag     2820

aaugcgaacg aaaaagaggu uaucgcuaag uccauuggca uccagaaucu uaagguguuc     2880

gaaaaauaca ucgucagcgc ccugggggag gugaccaagg ccgaauuuag acaacgcgag     2940

gacuucaaaa acgagcuccg guga                                            2964


<210>  3733
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3733
auggccagaa uccuggcuuu cgacauuggc aucagcucca uugggugggc cuuuucugag       60

aacgaugaac ucaaggacug cggagugcgg aucuucacca aagcggagaa ucccaagaca      120

ggcgaaagcc uggcccuucc ucgcagacug gcucgguccg cccgcaaaag acucgcucgg      180

cgcaaggcca gacugaacca ccuuaaacau cugauugcua augaguuuaa gcucaacuac      240

gaagauuauc agucuuucga cgagagccug gccaaggcgu acaaaggguc ccugaucucu      300

ccguaugagc uccgguuucg cgcccugaau gaacuucuga gcaagcaaga uuucgcuaga      360

gucauccucc acauugccaa acggcgcgga uacgacgaua ucaagaacuc cgacgauaaa      420

gagaagggcg cuauucugaa ggccaucaaa cagaaugaag agaaacuugc gaacuaucag      480

agcguugggg aguaccugua uaaagaauac uuucagaagu ucaaagaaaa uucuaaggaa      540

uuuacgaacg ugagaaauaa aaaggagucc uacgaacggu guauugccca gagcuuccuc      600

aaggacgagc ugaaacugau cuuuaagaaa caacgcgagu ucggauuuuc uuucuccaag      660

aaauucgaag aggaagugcu cagcgucgcu uuuuauaaga gagcccugaa ggauuucucu      720

caucuugugg gcaacugcuc cuuuuucacc gacgagaaac gggcucccaa gaauagcccu      780

cuggccuuua uguucguugc ccucacaaga auuaucaacc ugcuuaauaa ccugaaaaac      840

accgaaggca uccucuacac aaaggaugac cugaaugcuc ugcucaacga ggugcugaaa      900

aaugggaccc uuacauauaa gcagaccaag aaacugcucg gacuguccga cgauuacgag      960

uuuaagggcg aaaaagggac guauuucauu gaguuuaaga aauacaagga guucaucaag     1020

gcgcuuggag accacucucu gagccaggau gaccucaacg agauugccaa agauaucacc     1080

cugauuaagg acgaaaucaa acugaagaaa gcucucgcca aguaugaucu gaaucaaaac     1140

cagaucgacu cccuuagcaa acuggaguuu aaggaucauc ucaauauuuc uuucaaggcc     1200

cugaaacuga ucacaccccu uaugcucgag ggcaagaaau acgacgaggc uuguaacgaa     1260

cugaaucuga aggucgccau uaacgaagau aaaaaggacu uucucccugc guucaaugag     1320

accuacuaua aggacgaggu gacaaacccc guugugcugc gcgcuaucaa agaauaccgg     1380

aagguccuua augcccugcu caaaaaguau gggaaagugc acaagauuaa caucgagcug     1440

gccagagaag ugggaaagaa uuacucccag cgcgcuaaaa ucgagaagga acagaacgag     1500

aacuauaaag ccaagaaaga ugcugagcug gaaugcgaga aacuuggccu caagauuaau     1560

agcaaaaaca uccugaagcu gagacucuuc aaagaacaaa aggaguuuug ugccuacucu     1620

ggcgaaaaga uuaagaucag cgaccugcag gaugagaaaa ugcuugagau ugaccacauc     1680

uauccuuacu cccggucuuu cgaugacagc uacaugaaua agguccuggu uuuuaccaag     1740

cagaaccaag aaaaacucaa ucagacgccg uucgaggcgu uugggaacga uuccgccaag     1800

uggcagaaaa uugaagugcu ggcuaagaau cugcccacaa agaagcagaa gcgcauccuu     1860

gacaaaaacu auaaggauaa agagcaaaag gacuucaaag aucggaaucu caacgacacc     1920

agauacaucg cccgccuggu gcugaauuau acaaaggauu accucgacuu ucugccucuu     1980

agcgacgaug aaaacaccaa gcugaaugac acccagaaag gaucuaaggu ccauguggag     2040

gcuaaauccg gcaugcucac aagcgcccug cggcacacgu ggggguucuc ugcgaaggau     2100

agaaacaauc aucugcacca cgccauugac gcuguuauca uugccuaugc uaacaacucc     2160

aucgugaaag ccuuuagcga uuucaagaag gagcaggaau ccaauucugc cgagcuuuac     2220

gcuaaaaaga uuagcgaacu cgacuauaaa aacaagcgca aauuuuucga gcccuuuucc     2280

ggauuccggc agaagguccu ggauaagauc gacgagaucu ucgugucuaa accugaaaga     2340

aagaaaccca gcggcgcgcu gcaugaggaa accuuucgga aggaggaaga guucuaccaa     2400

uccuacgggg gaaaagaggg cguucucaag gcccuggaac uuggcaaaau ucgcaaggug     2460

aaugggaaga ucgucaaaaa cggagauaug uuuagagugg acauuuucaa gcacaaaaag     2520

acaaauaaau uuuaugcugu cccgaucuac accauggauu ucgcccugaa ggugcucccu     2580

aacaaggccg uugcucggag caaaaagggc gagauuaaag acuggauccu gauggaugaa     2640

aauuaugagu uuugcuucuc ucuguacaag gacucccuua uccugauuca gacaaaagac     2700

augcaggaac ccgaguuugu guauuacaac gccuucacca gcucuacggu cucccucauc     2760

gugagcaagc augauaauaa guuugagaca cugucuaaaa accagaagau ucucuucaaa     2820

aaugcgaacg aaaaggaggu uaucgcuaaa agcauuggga uccaaaaucu gaagguguuc     2880

gaaaaguaua ucgucuccgc ccuuggagag gugaccaaag ccgaauuuag acagcgcgag     2940

gacuucaaga acgagcugcg guga                                            2964


<210>  3734
<211>  2979
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3734
augagaaucu ucagcgacgg ccggaacccc aaggaugggu ccucucuggc cgugacccgc       60

agagcugccc gggcgaugcg cagacggcgc gacagacucc ugaaacggaa gacacgcaug      120

caggcuaaac uugucgagca cggauuuuuc ccugccgaug ccggcaagag aaaagcucug      180

gaacaacuca auccguacgc ccugcgggcu aaggggcuuc aggaggcccu gcuccccgga      240

gaauuugcgc gcgcccuguu ccauauuaac cagagacggg gcuuuaagag caaucgcaaa      300

acggacaaga aagauaacga cuccgggguu cugaagaaag cuaucggaca gcucagacaa      360

cagauggccg agcagggcuc ucggaccgug ggcgaauauc uguggacaag acuucagcaa      420

gggcagggag ugcgcgcucg guacagagag aagccuuaua ccacagagga aggcaagaaa      480

cgcauugaua agagcuacga ccuguauauc gaucgggcca ugauugagca ggaguucgac      540

gcgcucuggg ccgcucaggc cgcuuuuaau cccacccugu uccacgaggc cgccagagcu      600

gaucuuaaag acacacugcu ccaucaacgc ccucugcggc ccgucaagcc ugggagaugc      660

acccugcucc cggaggaaga acgcgcgccc cuggcccuuc cuuccacgca gcgguuuaga      720

auccaccagg aagugaacca ucugcgccuc cuggaugaga aucuucggga aguugcucug      780

acccucgccc aaagagacgc cguggucaca gcucuggaga ccaaagccaa gcugucuuuc      840

gagcagaucc ggaaacuccu gaaacuuagc ggauccgugc aguuuaaccu ggaagacgcg      900

aagcgcacag agcucaaagg caaugcuacc agcgccgccc uggcuagaaa ggaacuuuuc      960

ggggccgcuu ggucuggauu ugaugaggcc cugcaggacg aaauuguuug gcaacucgug     1020

acggaggagg gcgaaggcgc gcugaucgcc uggcugcaga cacacaccgg ggucgaugag     1080

gcucgggccc aggcuauugu ggacgugucc cuccccgaag gauacggcaa ccugagcaga     1140

aaagcccuug cgcgcaucgu cccugcccug cgggcugccg uuauuacaua ugauaaggcu     1200

gugcaggccg ccggguucga ccaccauucu caacucggau ucgaguacga ugcuuccgaa     1260

guggaggacc ugguccaccc cgagaccggc gaaaucagaa gcguguuuaa acagcugccg     1320

uacuauggga aggcgcuuca gcgccauguu gccuucggau cuggcaagcc ugaggauccc     1380

gacgaaaaga gauacggcaa gauugcuaau ccuaccgugc acaucgggcu caaccagguc     1440

cggauggugg uuaaugcccu gauccgcaga uauggacggc ccacagaggu ggucauugag     1500

cuggcccgcg aucucaaaca auccagagaa cagaaggugg aggcucagcg gcgccaggcc     1560

gacaaccaaa gacggaaugc gcgcaucaga cggagcauug cugaaguccu gggcaucggg     1620

gaggaaagag ugcgcggauc cgacauucag aaauggaucu guugggagga gcuuucuuuu     1680

gaugccgccg accggagaug ccccuacagc ggcguucaga ucuccgcugc caugcugcuc     1740

agcgaugaag uggaggucga acacauucug ccuuucucua agacgcugga cgauucccuu     1800

aacaaccgca ccguggcgau gcggcaagcu aauagaauca agcgcaaccg gacaccgugg     1860

gacgccagag ccgaguuuga agcucagggg uggagcuaug aggauauucu ccagcgcgcc     1920

gagcggaugc cccugagaaa acgguaccgc uucgcuccug acggcuauga aagauggcug     1980

ggagaugaca aggauuuucu cgcccgggcg cugaaugaca cccgcuaccu uucuagaguu     2040

gccgcugagu accugcggcu cguguguccc ggcacaagag ucaucccugg gcagcugacc     2100

gcccugcuuc gcggaaaauu cggccucaac gaugugcugg ggcuggacgg agaaaagaau     2160

cggaacgacc auagacacca ugcuguggau gccugcguua uuggcgucac ggaccaaggg     2220

cucaugcagc gcuuugccac agcuagcgcg caggcccggg gcgauggacu gaccagacuu     2280

guggacggca ugcccaugcc guggccuacc uaucgcgauc acguggagcg ggcugucaga     2340

cauaucuggg ugucccaccg ccccgaccac ggguucgaag gagccaugau ggaggagaca     2400

ucuuacggca ucagaaaaga ugggagcauu aagcagcggc gcaaggccga cggauccgcu     2460

ggcagagaaa ucagcaaucu gauucggauc caugaggcca cccaaccucu ccgccacggg     2520

guuucugcgg auggccagcc ccuggcuuau aaaggauacg ugggcggguc caacuauugu     2580

auugaaauca cagucaauga caagggaaaa ugggagggcg aggugauuag cacguuuaga     2640

gccuacgggg uugugcgggc cggaggcaug gggagacugc gcaacccuca ugaaggccag     2700

aauggacgga aacuuaucau gagacucguc aucggcgauu cugugcgccu ggagguugac     2760

ggggcugaac ggaccaugag aauugugaag aucuccggaa gcaacggcca gauuuucaug     2820

gcccccaucc acgaggcgaa ugucgaugcu cggaacacag acaagcaaga cgccuuuacc     2880

uauacaucca aauacgccgg gucucugcag aaggcuaaga cccgcagagu gacgauuagc     2940

ccgaucggag aagugcggga uccuggcuuc aaaggcuga                            2979


<210>  3735
<211>  2985
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3735
augacccugg gccucgaccu ggggacaaac agcaucggau gggcccuuau ugauuucgac       60

gauaaucagc aacccagaaa ccugaucgcu ugcggcuccc ggauuuuuca ggaggccgug      120

gaagcgaaga cggggacccc uaaaaaucac gcucgcagaa cagcccgggc cgcucgcaag      180

cucgucgcca gacggaaaca gcgcagaacc aagcugcuua accugcucau ccggcaugga      240

cugcugccgc aggacguugc ugagcgcaau gcccuccugc uugauaacca agcgaauaac      300

cccuaccagc ugagaaaacg gggccucgac gaaaagcugg agccuuucgc cuuugggcgc      360

gcucuuuauc accuggccca uagacgggga uuccagucua aucgcaaggc ugccagcgau      420

gaagacggca aagugaagac agcgauuucc acccucagag ccgagaugca gggccggaca      480

cugggggcuu uucuggccga ucaacccacc cagagacgcc gguacacgga cagagcuaug      540

uauguggagg aguucgagca gcucuggucu aaacaggcca gcuuuaccca agaacagcug      600

acacaggccc uuaaggucgc uauccacaac uccaucuucu uucaacgccc ucugaaaucu      660

cagaagcauc ucgugggaaa guguaccuuc gagcccacac ggaaaagagc ggccaaggcu      720

auucugcuua cccagcgcuu ucggauccug caggauguua aucaccucgc cgugaaaaac      780

ccuauuacga gagaguuccg cccgcugaca caagacgaac ggaauaagcu ggccgaggcu      840

cucgauaaac aaaagagcau gaccuggggc gccgucagaa aagugaucgg guggaacaag      900

aaggacuccc ugcacgaagg agagacauuu aaucuugaag agggcaaaaa ggacaaacug      960

auugggaacc gcaccgcgug cgaucuccgg ggagcucugg gcgacagaug ggaugcccuu     1020

accgcccagc agcaagacga ucugguuaca gaccuccuga cgaucgauaa caccgacggc     1080

uuccugaagc ggaugaaaac acauugggag uucgaugacg cuaccgccga agugauugcu     1140

aagaucgagc ucgaacccgg guacgcccgc cugagcgcga aggccaugag agcuauccuu     1200

ccuuaucugg agcagggaau gauuuacucu gaugccugua aagcugccgg cuaugaccac     1260

uccaauccca accagcggac aaucaccgac aagcucgggc ugccuagcaa ugucagaaac     1320

cccguggugc agaaagcgcu guuugaaacg cgcaaggucg uuaaugccau ugugcggaaa     1380

uacggaaagc cggcugugau cagagucgag auggcccgcg auaugaagcu cucuaaaaga     1440

caaaaggagg aacuccagaa agagcagaac aagcagaaaa aggcuaauga acuggccgag     1500

ggcauucugc ggaaggaguu cgggauccaa cagccugccc gcgcugacau ccagaaauac     1560

aaccucuggc uggaaugcaa ggaggugugu cccuauacag gaaccaccau uuccagagaa     1620

augcuuuuua gcccugaggu ugauguggaa cauauccugc ccuacucucg gucccucgac     1680

gauagcuaua ugaauaaaac acugugcaug gcgaccuuca accgccaagu caagcugaau     1740

aacacacccu acgagauugg ccagggcgac ccucuugccu augcugagau cuuucagaga     1800

auuaaaacgc ucccguuccc caagcggcgc agauuugaac agaaagagau cgaaaccgau     1860

aaguucaucg agcggcaacu gaaugacaca cgcuacauuu guguggaagu uagagauuuu     1920

cugcagcagc ucggggcccc uguggagguc uccaagggag aggccaccgc uaaacugcgg     1980

ggcagaugga aacuuaacac acugcucucu cccgacggga gcaaugaaaa guaucgcgga     2040

gaucaccggc accaugccau cgacgcggug gucauugcuc ugaccaacag aucccuguuc     2100

cacaaacuua gccgccucuc ugcccagucc aucagccugg auaagucucg guuugacgug     2160

ccucccccgu gggacggcuu cuacgaugac guuuacgaga agauugauaa ggugaucguc     2220

agccaugccc cugcuagaaa aauugggggc gcccugcacg aagacacggc guauggacau     2280

uuuaaggagg aaguggcuga ucccaaaacc ggcaagguuu ccaucgagga ccgcuucgug     2340

uaccggaaac cucucgccac acugaccgcc aacgaggucg ggaagaucag agaugcuaag     2400

gugcgcgagu ucguggaagc ccggcuugcu caauuuggaa auaacucuaa aacagccuuc     2460

ggcgacccca auaacccucu gcuccacgcg gaugggaaga cccccauuag aacgguucgg     2520

cuggucacaa auuuuaacaa agccaccaca cacgcuaucc agaagggaca gggccgcgac     2580

uauaaauucu uuaaguacgg gaauaaccau cacguggaaa uuaucgagca uguggaaacc     2640

ggcaagagaa aaggaauuuu cgucaccgcc auggaggcug cccggcgcgu gagaggcguu     2700

gccaauacag aaaagagcga uaucgugcag cggaaccacg gggaagagug gagauuuguc     2760

augucccuga gcaucaauga ccuugugucu auucaagaag cugaucaggu ucgcaucaug     2820

cgggugcagc uuuucgacgc guccaacgga aauauuaucu uuagagacca gaaaagcgcc     2880

augcuggaug acaagcuguc ucgcuaucag acgaaagcuc accuccuggu cugccagaag     2940

auuaccacag auccgcuugg caaccuggcc ccuuguaaug acuga                     2985


<210>  3736
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3736
augagcaagg ugcugggccu cgacaucggg gccaacucca uuggaugggc ucugaucgau       60

gacgaggaaa augauauuau cggcgccggg gucagaguuu ucaaagaggg agugaaccuu      120

aagggcguga aagaagaguc uaagaauguc gaacggcgcg cggcuagacu ggcccggcgc      180

cagcacaaaa gacggagcca acgcagaaac cggcucgugg cccugcuuaa gacccuggag      240

auguuucccg agaauaacga agacauuucc gauuacuaug cucaggaccc uuacgagcuc      300

cgcaagaaag ggcuguauga aaagcugaca auguacgagu ucggaagagu ucuccugcau      360

aucugccggc gcagaggcuu uaaaucuagc cggaagacgg auaucuccga ggaaggcaaa      420

auuuucgagg ggaaggacgg aaucgugggc auuaaucaga cccgcgauuc uaucagagaa      480

gggggauuua agacaauugg cgaguaucuu gccagccugg acccgcacgu cauccggaga      540

cgcaaccggu ucacccucag agauauguac gaagaggagu uuaaucugau gugggaaaaa      600

cagcaauccu ucuauccccu ucagcugagc aacgaguuua aggaagcuau ugccgaggug      660

auccucucuc agcgcaaacu gaagucccag aaagcgcugg uugggaauug uggacucgaa      720

ccuaacaaga aaacagcccc caagagcucu ccugcuuucc aauacuuucg gauccuggag      780

cagcuuuccc gcauucugau cagcauuccc cauaccggca gauacaauga cccucucaac      840

gaggaggagc ggagaaugcu gaucgauaaa cuuucuguga cagaaaagcu gaaguucucc      900

gacaugcgca aaaugcucaa ucugccgggc gagagcgaca uuaacaucga agaggaaggg      960

ggacggcugc ucggcaacac cacguaugcc agacugauca aggucuuugg gaaaaagcgc     1020

ugggcuggau ucuccgaaga ggaaaaggag cagauuuggc acaccuucca uuuugccgau     1080

aaucacgagu ggcuuaaaga auacgcgcug aagaaauggu cuaucacacc cgacgagauu     1140

aaggaacucg gcaaaguggc ccuggagaag gaauaugcuc gguucagcaa gaaaucccuu     1200

aagaaaauca uuccucagcu ggaggagggg cucacccugg auaaggccaa aaucgaagcu     1260

ggauacacac ccgaguauga aaucgaaaag agccugucug ccuaccuccc ucugcccgag     1320

aacauuagaa auccgaucgu gcaacaggcc cuuuaugagc ugcggcacgu cguuaacgcu     1380

gugauucgca ccuacggcaa gccugacauc gcgagagugg agcucgcccg ggaaaugaaa     1440

aagcccaaac agaagagaga gaaaaugcgc uccgaacugc ggaagagaca aaugaaauuu     1500

gaggaaauua agagcauccu gaaggagcgc cuuggcuuca uugauccuaa aagagacgag     1560

auccauaagu acaugcucug ggaagagugc cgggaaacgu gucccuauac agggcgcaaa     1620

aucucucuga gcgcucugua cggaggcgau uuugaggucg agcacauuau ccccuauaag     1680

agaucccucg acgauucucu guucaauaaa accgugugcc uucgggaaga gaaccagauu     1740

aagggggacc gcacaccuua cgaagccuau agcggagaua gacagaagua cgaggaaauc     1800

auucugcggg uucagaaaac cuccaugccg cgcggcaaga aaaaucucuu ugccaucaag     1860

gagcugaaag acgacuucgc ucauagacaa cugagcgaua ccgccuauau cucucgggag     1920

acacgcaagu accuuggguc cacgauuaag aaagugcagg cggucaaggg cggaagcacc     1980

gcuaaacuca gauacuauug gggccugaac ucuauccugg gggugcggga cauuaagaug     2040

agagaugacc accgccacca ugccguugau gcccucguga ucgcuaauac aaccuacgcc     2100

uuugucaaca cacugucccg guaucacagc auggacgugg aaccccauga guccagauuc     2160

ccucuucccu gggaacaguu ucgcaaugau gcugagucug ucgugcggaa cauuuucguu     2220

agccacagac uggggaaaaa ggccaccggc cagcuccaug aagagacgua cuaugggaag     2280

auccgcaaua cagacggaga gccuuacuuu gugguccgga aaaagcuggg cacccugacc     2340

gcgaaacaaa ucgccaacau uguggauccc guugugaaga cacaggucuu cgaaagacuu     2400

gaggaaaaag ggaucgacau ugaguccucu aauaagaucc cggcugaugc cuucguggaa     2460

acccucuaua ugccugaccg caaaacaccc auuaagagcg ugcggguuaa ggucccuucc     2520

acgaccauga uccagcugua caaagauaag gagcuguacg uggaccccgg cagcaaccac     2580

cacaucguga uuuuugagaa cgaagaggga aaaagagacg gcaucgucgu gucucucuuc     2640

gaagcugccc agcggcugcg cagaaaugag ccuguuauuc aaaagacaug gaaacccgag     2700

uggcauuuug ugaugucccu uagcaucgau gaacugauuc uccuggacgu cgaugagaag     2760

aacaucgacu ggggagauac cggacuggcg cuucgguauu cuaaggcccu cuaccgcgug     2820

cagaaaaugg acguuaauaa gauuaucaca cugagacacc aucugguggc ucggcucaaa     2880

gaugacgaag gcaaggaguu cgggagagaa caccgcaucc cguccacccu gcggggacuu     2940

aaagucaaga uugaucacau cggcagacug cgccgggccu augacuga                  2988


<210>  3737
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3737
augaagguga ucggcuucga cauugggauc aacagcauug gaugggccuu ugucgagaau       60

gaugaacuga aagacugcgg cguuagaauc uucaccaagg cugagaaccc caaaaauaag      120

gaaucccucg cccugccucg gcgcaacgcg agaucuagcc ggaaacgccu uaagagacgg      180

aagcugcgcc ucauugcuau gaaacacauc cuggccaagg agcuuaaucu gaacuacaaa      240

gauuauaucg ccaaugacgg ggaacucccg aaggcuuacg agggaaaacu gauuuccccc      300

uaugagcuga gauacaaggc ccucaaccag aagcuggaag cuaaagaucu ugugcgggug      360

auccugcaua uugccaagca ccgcggcuau augaauaaaa acgacaagaa aucuaaugau      420

gcggagaagg ggaaaauccu cagcgcccug aagaacaaug cucuuaagcu ggaaaaauac      480

caauccgucg gagaguauuu uuacaaggag uucuuucaga aauacaagga aaacacaaaa      540

gacuucauua agaucagaaa uauuaaggau aacuauaaca auugugugcu cgccucugac      600

cuggagaaag aacugaagcu cauccuggag aaacagaagg aauuuggcua cagcuauucc      660

aacgauuuca ucgaggaggu uauucaggug gccuuuuucc aacggccucu uaaagacuuu      720

agccaucugg ucggcacgug caccuucuuu gaagaggaaa agcgcgcuug uaaaaauucu      780

uacuccgcgu gggaguucgu ggcccucaca aaaaucguua acgaacuuaa gagccuggag      840

aaaauuucug gggagcugau cuccacccag acaauuaaug aaauccucaa cuauauccug      900

gauaagggaa gcauuaccua caaaaaguuc agaucuuaua ucaaucugua cgaguccauu      960

aaguuuaaaa gccucaagua cgacaaagac aacgcugaau ccgccaagcu gaucgauuuc     1020

cggaaacuug uggaguuuaa gaaagcucug ggcgaacacu auuucaaucg ccaggagcuc     1080

gaccagauuu cuacauacau cacccugauc aaggauaacg ucaaacuuaa aacggcccug     1140

gagaaguaua aucucagcaa cgaacaaauu aauaaccugc uggagaucga auuuaaugac     1200

cauauuaacc ucuccuucaa agcccugagc augauccuuc cccugaugaa ggaggggaaa     1260

aaguacgaug aggcuugcga aauugcgaac cucaagccua aaaccacaaa ucagaagcag     1320

gacuuucugc ccgccuucug uaacucuauc uuugugcacg agcuguccaa uccuauugug     1380

aacagagcua ucagcgaaua ucggaaaguc cuuaaugccc uccugaagaa auacggaaag     1440

guucacaaga uccaucugga gcucgccaga gauauuggcc ugucuaaaaa ggcucgcgaa     1500

aagaucgaga aggagcagaa agaaaaccaa gccauuaaua ccugggcgcu uaacgagugc     1560

gaaaauaugg ggaucaaggc uaacgccaag aauauucuga aacucaagcu guggaaagag     1620

cagaaggaaa ucuguaucua uagcggaaag aagauuucca ucgagcaccu gaaagacgag     1680

aaggcccuug aaguggauca uauuuacccg uacucucgga gcuucgacga uuccuauaug     1740

aacaaggugc ucgucuuuac aaaagagaau caggaaaagc ugaacaaaac ccccuucgag     1800

gcuuuuggca agaauaucga aaaauggaac aagauucaaa cgcuggccca gauccucccu     1860

uacaagaaaa agaacaaaau ccuggacgag aauuucaagg acaaccagca gcaagauuuc     1920

auuagcagaa aucuuaacga cacacgcuau aucgcuaccc ugauugccaa auacacaaag     1980

gaguaucuca auuuucugcc ccugaacgaa aaugagaaca ucaaucuuaa gucuggcgaa     2040

aaagggucca agauucacau ccagaccgug aacggaaugc ucaccagcgu ucugcggcac     2100

acauggggcu ucgauaaaaa ggacagaaau aaccaucugc accaugcgcu cgaugccauc     2160

auuguggcuu acucuaccaa uucccugauc aaagccuuua gcgacuucaa gaagaaccag     2220

gagcuucuga aagcuaagcu cuaugccaaa gagcugacca augauaacua cauucaccag     2280

gcgaaguuuu ucgaaccuuu ugagggguuc cgcgagaaaa uccugaacaa gauugacgaa     2340

aucuuugucu ccaagccccc gcggaaaaga gcccgcggag cccuucauaa ggaaacauuc     2400

uacucugaga aaaagaugau uaaaaaguau aauagcaaag agggccucaa caucgcucug     2460

gauugcggga agauccggaa gauuggaacc aaauacgugg aaaaugacac aaugguuaga     2520

guggauaucu uuaagaaaca aaacaaguuc uaugcuauuc cuaucuacac cauggacuuu     2580

gcccugggca uucuccccaa uaaaaucguc gugaucggca aggauaagaa caauaacccu     2640

aaacaguggc aggagauuga cgaauccuau gaguucuguu ucucucugua caagaaugac     2700

cuuauccugc uccagaaaaa ggauaugcaa gaagccgagu uugcuuauua caacggguuc     2760

gacauugaua gcucccgcau caauauuaaa aagcacgaca acaaguuuga gaaucugacg     2820

agcaaccaga aacugcuuuu cacaaauaag gagaaaaacc ggaccggaau ccagaaucug     2880

aagaucuuug aaaaauacau ugucacgccc cucggcaaca agaucaaggc ggauuuccag     2940

cccagacaaa acauuucucu gaaauccagc aagaaaaaug ggcucuga                  2988


<210>  3738
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3738
augaagaucc ugggcuucga cauugggauc aacagcauug gaugggccuu uguggagaau       60

gaugaacuca aagacugcgg cgucagaauc uucaccaagg cugagaaccc caaaaauaag      120

gaaucccugg cccuuccucg gcgcaacgcg agaucuaauc ggcgcagacu gaaacggcgc      180

aagagcagac ucauugcuau caagcacauc cuggccaaag agcuuaaccu gaauuacaag      240

gauuauauug ccaacgacgg ggaacucccg aaggcuuacg agggaaagcu gacaaauguu      300

uaugagcugc gguacaaagc ccucaaccag aagcuggaag cuaaggaucu ugcccgcgug      360

auccugcaua uugcgaaaca cagaggcuau augaauaaga acgagaaaaa guccaaugac      420

ucugaaaaag ggaagauccu cagcgcccug aaaaacaaug cucuuaagcu ggagaaguac      480

caauccgugg gagaguauuu uuacaaagag uucuuucaga aguacaaaga aaacacgaag      540

aacuucauua aaauccggaa uaccaaggau aacuauaaua acuguguccu cgccucugac      600

cuggaaaagg agcugaaacu cauucuggaa aagcagaaag aguuuggcua cagcuauaau      660

gaugacuuca ucaaggagau cauuaacauc gcuuucuucc agcgcccccu uaaagauuuu      720

ucccaucugg ugggcagcug cacauucuuu gaagaggaaa agagagccug uaaaaauucu      780

uacuccgcgu gggaguucgu ugcccucagc aaaauuauca acgaacugaa gcggcuugag      840

aaaauuucug gggagcugau cuccgcucaa accaucaaug aaauucucaa ccacauccug      900

gacaagggaa auauuacaua uaaaaaguuc cgcagcugca ucaaccugca ugagucuauu      960

gaauuuaagu cccucaauua cgauaaagac aacgccgaga gcgcuaagcu gguggacuuc     1020

aaaaagcuga ucgaauuuaa aaaggcccug ggcgagcacu ccuucaauag acaggagcuc     1080

gaugaaaucg ccacccacau uacacugauc aaagacaacg auaaacuuaa gaccgcucug     1140

gagaaguaua accucaauaa cgaacagauu aaugcgcugc uggagaucga guuuaacgac     1200

cauauuaauc ucucuuucaa ggcccugaac cuuauccugc cucucaugcg ggaagggaaa     1260

agauacgaug aggcuuguga aauugucaau cuuaagagca aaacguccaa ccagaagcaa     1320

gaccugcuuc ccgccuuuug cgauagcauc uucucucagg agcucuccaa uccugugguu     1380

aaccgcgcca ucagcgaaua ccggaaggug cugaaugcuc ugcucaaaaa guauggaaaa     1440

guccacaaga uucaucugga gcuugccaga gacgugggcc ugucuaaaaa ggcgcgcgag     1500

aagaucgaaa aagagcagaa ggaaaacaaa gagauuaaug auugggcucu cgacgaaugu     1560

gagaaguuug ggcugaaagc caacaauaag aacauccuga aacuuaagcu cuggaaggag     1620

cagaaagaaa uuugcgccua cagcggaaag aaaaucucca ucgagcaccu gaaggaugaa     1680

aaagcucugg agguggacca cauuuauccc uacucucgga gcuucgacga uuccuauaug     1740

aacaaggucc ucguuuuuac caaggaaaau caagagaaac ugaacaagac accuuucgag     1800

gccuuuggca aaaauaucga aagauggaac aagauucagg cucuugccca gaaucugccg     1860

uacaaaaaga agaacaaaau ccucgacgag aauuucaagg auaaacaaca gcaggacuuc     1920

auuagccgca accugaauga uacccgguau aucgcgacac ugaucgccaa guacaccaaa     1980

gaauaccuua acuuucuccc ccugaaugag aacgagaaua cgaaccugaa gucuggcgaa     2040

aaggggucca aaauucaugu gcagacaauc aauggaaugc ucaccagcgu gcugagacac     2100

acauggggcu ucgacaagaa agaucgcaac aaccaucuuc accaugcucu ggacgccauu     2160

aucgucgcuu auucuaccaa uuccauuauc aaggccuuua gcgauuucaa aaagaaccaa     2220

gagcuccuga aggcgaaacu guacgccaag gaacuuaccu ccgacaauua uaaacaccag     2280

gcuaaguuuu ucgagccuuu ugaaggguuc cgggagaaaa uccucaacaa gauugaugag     2340

aucuuugugu cuaagccccc uagaaaacgc gcccggggaa cacugcacaa ggaaacguuc     2400

uaccccgagg aaaccaugau uaaaaaguau aauagcaaag agggccugaa caucgcucuc     2460

aauuguggga agauuagaaa aaucggaaca aaguacguug aaaacgacac cauggugcgg     2520

cuggauauuu uuaagaaaca gaauaaguuc uaugccaucc cgaucuacac aauggacuuu     2580

gcccuuggca uucugccuaa caaaaucguc auugcuggca aggacaaaaa uaacaauccc     2640

aagcaguggc aagagaucga ugaguccuac gaauuuugcu ucucucucua uaagaacgac     2700

cugauucugc uucagaaaaa ggauaugcag gaggcggaau uugccuacua uaauagcuuc     2760

gacaucgauu ccagccgcau caacauuaaa aagcaugaca auaaauuuga gaaccugacc     2820

gauaaccaga agcuccuguu cacgaaucaa gaaaagaaca gaacagggau ccagaaucuc     2880

aaaauuuuug agaaguacau cgugaccccu cugggagaca aaauuaaggc ugauuuccag     2940

ccccggcaga acaucucucu uaaaaccucc aagaaguaug gccuguga                  2988


<210>  3739
<211>  2991
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3739
augaugggca acuucacccu ggggcucgac cugggaacaa auagcaucgg cugggcccuu       60

auugauuuug acgauaacca gcaaccccag aaucugaucg cuugcggguc cagaauuuuc      120

caggaggccg uggaagcgaa gacgggaacc ccuaaaaacc acgcucggcg cgccgccaga      180

gcugcccgga agcucgucgc ucgcagaaaa cagcggcgca caaagcugcu uaaucugcuc      240

guuugucaug accugcugcc gcaagaugag gccgaaagaa acgcgcuccu gcuugacaau      300

caggccaaca aucccuacca gcugcggaaa cgcggccucg augagaagcu ggaaccuuuu      360

gcuuucggga gagcccuuua ucaccuggcu caucggcgcg gauuucaguc uaacagaaag      420

gccgcgagcg acgaggaugg caaagugaag accgccaucu ccgcucuccg ggccgagaug      480

caaggccaca cacugggggc uuuccuggcc gaccagccca cccagcgcag acgguacaca      540

gauagagcca uguaugcuga agaguuugaa cagcucuggu cuaaacaaac cauuuucgcg      600

ccugagcagc ugacgcaggc ccuuaaggug gagaucaaaa auagcaucuu uuuccaacgc      660

ccccugaagu cccagaagca ucucgucgga aaaugcaccu uugaaccuac acggaagaga      720

gcugccaaag ccauucugcu ugcucagcac uuccgcaucc ugcaggacgu gaaccaccuc      780

gccguuaaga auccgauuac ccgggaguuu agaccccuga cacaagauga acgcaacaaa      840

cuggcggagg cucucgacaa gcagaaaggc augaauuggu cugccgugcg gaaggucauc      900

ggguggaaca agaaagacag ccugcaugaa ggagagaccu ucaaccuuga ggaaggcaag      960

aaagauaagc ugauugggaa uagaacggcc ugugaccucc gcggagcucu gggcgaucgg     1020

ugggacgccu ucacagcucc ucagcaggau gaccuuguga ccgaucugcu cacaaucgac     1080

aaccccgaug gcuuucugaa aagaaugaag acccacuggg gguucgacga ugccaccgcg     1140

gaggcccugg cuacaacgga acucgagccu ggauacgccc gccuguccag caaggcuaug     1200

cgggccauuc uuccccaucu ggaacaaggc augaucuauu cugacgcgug caaagccgcu     1260

ggguacgacc acuccaaucc gaaccagaga accaucacag auaagcucgg acugccuagc     1320

aauguucgga accccguggu ccagaaagcc cuguuugaga cccgcaaggu ggugaaugcu     1380

auugucagaa aauauggcaa gccugccguu auccgggugg agauggccag agacaugaaa     1440

cuuucuaagc gccagaagga ggagcuccaa aaagaacaga acaagcagaa aaaggcuaau     1500

gagcuggcgg aagggauucu gcggaaggag uucggaaucc agcaacccgc cagaacagau     1560

auucagaaau acaaccucug gcuggagugu aaggaagugu gccccuacac cggcacgguc     1620

aucucccgcg agaugcuuua uagcccugaa guggacguug agcacauccu gccguacucu     1680

agaucccucg augacagcua uaugaauaaa acacugugua uggcuaccua caaccggcag     1740

accaagcuga auaacacacc cuaugagauu ggccaagggg auccuguggc cuacgccgaa     1800

aucuuucagc gcauuaaaac ccuucccuuc ccuaagagac ggcgcuuuga gcagaaagaa     1860

aucgagacag acaaguucau ugaaagacag cucaaugaua cgcgguauau cugcgucgag     1920

gugcgcgacu uucugcaaca gcugggagcu cccguugagg uguccaaggg cgaagccacc     1980

gcgaaacuca gagggcggug gaagcugaac acacuucugu cuccgggagg cgggaacgag     2040

aaaaaucgcg cugaucauag acaccaugcc aucgacgccg ucgugauugc ucucaccaac     2100

cggagccugu uccacaagcu guccagacuu agcgcccagu cuaucuccuu ugauaaaagc     2160

cgcuucgacg ucccuccccc uugggacgau uuuuacgacg auguguacga aaagauugac     2220

aagguuaucg ugucucaugc gcccacacgg aaaauuagcg gcgcucucca cgaggccacc     2280

gccuauggac acuucaagga agagaucgcu gauccuaaaa cgggcaaguu cuccauugag     2340

gacagauuug ucuaccgcaa accccugacc acacugaccg ccaaugaagu ggggaagauc     2400

cgggaugcua agguuagaga gcucguggaa gcccgccugg cgcaguucgg aaacaauucu     2460

aaagccgcuu uuggcgaccc gaacgagccu cuucugcaug ccgaugggaa gacacccauc     2520

cggaccauua gacucacgac aaauuucaac aaaaccacaa cccacgcuau ccaaaaggga     2580

caggaccgcg auuauaaauu uuucaaguac ggcaauaacc aucacgucga aauuaucgag     2640

cacguggaga ccgggaagcg gaaaggcauu uuugugacag ccauggaagc cgcuagacgg     2700

gcgcgcggag uugccaauac ggaaaagagc aacaucgucc agagaaauca uggcgacggg     2760

uggaaauucg ugaugucccu ggcugugaac gauauggucc ggaccgaaga cacacaggcc     2820

aucuaucgcg ugcaaaagcu ggacggaagc aauaacagau uuacccuucg gucucacaac     2880

gccgcuacac ucgaugacaa acagaccggc guugauaaga cgcccaaucu guucaaaggg     2940

acaaagauua ucguggaccc ucugggaaac cucacccagu guaaugauug a              2991


<210>  3740
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3740
augaaggugc uggucgacgc cggccucaug cccagcgauc cugcugccgc gaaagcucug       60

gagacccuug acccguacga acugagagcc acaggccucg auguuaugcu gccccuuacg      120

caccugggac gggccguguu ccaucucaac cagcgcagag gcuuuaaguc caaucggaaa      180

accgaccgcg gggauaacga gucuggaaag aucaaagacg cuacaaagag acugaccugg      240

gccaugcaag aagcuggcgc ccggacauau ggggaguucc ugcacaugcg cagagcgcgg      300

gccagcgauc cucgccaggu gcccuccguc agaacccggc ucucuguggc uagccgcgga      360

ggcccugacu ccaaggaaga ggccggcuac gauuuuuauc ccgagagagc ucaucuggaa      420

gacgaguucc acgcccuuug gucugcgcag gccgcuuuuc auccugaucu gacaugcgaa      480

cuccgggacg uuguguucga gaaaaucuuu uaccagcgcc cgcugaagga gcccaaaguc      540

gggcuuuguc uguucaccgc cgaagagaga cucccuaagg cucacccccu gacgggacgg      600

agagugcugu augaaaccgu uaaucaacuc cgcgugacag ccgauggccg gaccacaaga      660

ccucugaccc uugaggaacg cgacaugauu gucuacgccc uggacaacaa aaagcccguc      720

aagagccucu ccagcaugca ccugaaacuu guggcucugg ggaagcucgu gaaacugaag      780

gauggagagc gguuuacgcu ggagucuggc guuagagacg cgauugccug cgauccgauc      840

cgcgcuacaa uggggcaucc ugacggauuc ggccccgccu ggucccggcu cgauuggcag      900

gcccaguggg cucugauuga aaaacuuaga caggugcaaa gcgacgauga cuuugccucu      960

cugguggcgu ggcucggcgc uacccacaau cuguccgagg aucaugccaa gcgcgucgcc     1020

aaccuucauc ugccugaagg guauggacgg cucggccuga cagcuaccga ggccauccug     1080

uguaaucuca aagcugaagu gugcaccuac agccaggccg uugagagaug ggggaagcac     1140

cacucuaacc agcgcacagg agagugucug gaagcgcuuc ccuacuaugg cgaggugcug     1200

gaccggcacg ucauccccgg gacguacaau gaagaugacg acgaugugac cagauaugga     1260

cggauuacaa acccuaccgu ucauaucggc cucaaucagc ugcgcagacu ggugaaccgg     1320

auuaucgcca cauacggcaa gccggaccaa auugucgugg agcuugcuag agaacucaaa     1380

caguccgagg cccaaaagag cgaggcucug aaaaagaucc gcgauaccac ggccgacgcg     1440

auucggagag ggcgccagcu ggaagagauc ggacagaaaa auacaggcgc caacagacuc     1500

cugcuucggc ugugggaaga ucucgggccc gcugucggac cucgcugcug ucccuauacc     1560

ggcaagccua ucuccgugac caugcuguuc gacggggccu gcgauguuga ccacauucug     1620

cccuacucua gaacacuuga ugacagcuuu gcuaaucgga ccaucugucu ccgcgaggcc     1680

aacagacaga agggcgaucg gacaccgugg gaggccuggg gaggcuccga ccagugggau     1740

gcuauugaag cgaaccugaa agaccugccu gagaauaagc gcaaaagauu cgcccccgac     1800

gcuauggaac gguucgaggg ggaacgcgau uuucucgaca gagcccuggu ggauacgcaa     1860

uaucuugccc ggaucagcag agcuuaccug gacacccucu ucacagaggg aggccauguc     1920

ugggugguuc cugggcgcau gaccgagaug cugcggagac acuggggacu gaacgcccuu     1980

cucucugaua aggaccgcgg cgcggggaaa gugaagaauc ggacagauca cagacaucac     2040

gcuauugacg ccgccgucau cgcugccacc gaucauuccc uggugaaccg caucagcaag     2100

gcggcuggcc agggagaauc ugccggccag agcgccgagc ugauugcucg ggacacgccc     2160

gaaccuuggg agggguuuag aaaugauauc uccgcccaga uugcucgcau cgugguuucu     2220

caccgggccg accacggaag aauugauguc gaaggccgga aacaugggaa ggacagcacc     2280

gcgggacgcc uccacaacga cacagccuac ggcaucgugg augaccauac cguggucucc     2340

agaacacccc ugcuuagccu gaaaccgggg gauaucgagg ugaccacgaa gggcaaaaau     2400

auucgggaca cacaacucca gaaggcucug ucuguugcca ccacaggaaa ggauggcaaa     2460

ggguucgagg aagcucugcg caacuuugcc gagaaggaag gaccuuauca gggcaucaga     2520

cgggugagac uuauugagac ccuccagucc agcgcccgcg ucgaaguggg gucugacgag     2580

gauggacaac cccugaaggc uuacaagggc gacuccaauc acugcuauga ucuguggaaa     2640

cuccccgacg ggaagguugu gccucauguc gugagcaccu acgaggcgca cgccggcaca     2700

gauaaacggc cgcaccccgc ugccaagaga auccugcgcg uuuucaagaa agacauggug     2760

gccauugaac aggacgauca gacggcuauc uuuuaugucc agaaacuuga ccgggccaac     2820

ggacuguucc ucgcgccuca ucuggaggcu aaugccgaug ccagacaccg caaccccaau     2880

gacgcuuuua aauuccugca aaugggcgcc ggguccgugg ugcggaacaa acuuagacgc     2940

gucuacguug augaaauugg aagaaucaag gacccugggc ccccuaagca uuga           2994


<210>  3741
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3741
augaagaucc ugggcuucga cauugggauc aacagcauug gaugggccuu uguggagaau       60

gaugaacuca aagacugcgg cgucagaauc uucaccgagg cugaaaaccc cuccaauaag      120

gagucucugg cccuuccucg gcgcaacgcg agaagcuccc ggcgcagacu gaaacggcgc      180

aaggcuagac ucguugccau uaaacggauc cuggccaagg aacuuaagcu gaauuuuaaa      240

gauuacgugg cugccgacgg ggagcucccg aaggcuuaug agggaaaacu gacaucuauc      300

uacgaacugc gcuauaaggc ccucauucag caacuggaga cgaaagaucu ugcgagagug      360

auccugcaca uugccaagca ucggggcuac augaacaaga augagaaaaa gagcaacgac      420

accaaaaagg ggaaaauccu cuccgcucug aagaauaacg cccuuaaacu ggaaaauuau      480

cagucugucg gagaguacuu cuauaaggaa uuuuuccaga aguacaaaga gaacacaaag      540

aauuuuauua aaauccgcaa caccaaggau aauuacaaca acugugugcu cagcuccgac      600

cuggaaaaag agcugaagcu cauucuggaa aagcagaaag aguucggcua uaauuacagc      660

gaggauuuua ucaacgaaau ccuuaagguu gcuuucuuuc aaagaccccu gaaagacuuc      720

ucucaccucg ugggcgccug cacauuuuuc gaggaagaga agcgggcgug uaaaaauucc      780

uauagcgccu gggaguucgu cgcucugacc aagauuauca acgaaauuaa gucucuugag      840

aaaaucuccg gggaaauugu gccuacacag accaucaaug agauccugaa ccucauucug      900

gauaagggaa gcaucacgua caaaaaguuu cgcucuugca uuaaucugca ugaauccauc      960

agcuucaaau cccucaagua ugacaaggag aacgccgaaa augcuaaacu gauugauuuu     1020

agaaaacuug uugaguucaa aaaggcccug ggcgugcacu cucucagccg gcaggagcug     1080

gaccagaucu ccacccauau cacacuuauu aaagacaacg ucaagcugaa aaccgugcuc     1140

gaaaaguaca aucugagcaa cgagcaaauc aauaaccugc ucgaaauuga guuuaaugau     1200

uauaucaacc ugucuuucaa ggcccuuggg augauucugc cccucaugag agagggaaaa     1260

cgcuacuacg aggcuuguga aaucacaaac cugaagccua aaaccgugga cgaaaaggag     1320

gauuuucugc ccgcguucug cgacuccauu uuugcccacg aacuuagcaa uccugucguu     1380

aaccgggcua ucucugagua uagaaaagug cucaaugccc ugcugaagaa guacggcaaa     1440

gugcacaaga uccaucucga gcuggcccgc gaugucgggc uuagcaaaaa ggcucgggag     1500

aaaauugaag accagcagaa gaacaauaag gccaucaacg auugggcgcu gaaagaaugu     1560

gagaauauug gaaucaaggc uuccgccaaa aacauucuca agcugaaacu guggaaggaa     1620

cagaaggaga ucugcaucua uucuggcaau aaaauuagca ucgagcaccu uaaggacgaa     1680

aaagcccucg agguggauca uauuuacccg uauucccgca gcuucgacga uucuuuuauc     1740

aacaagguuc ugguguucac gaaagaaaau caagagaagc ugaacaaaac acccuuugag     1800

gcuuucggca agaauauuga aaaguggucc aaaauccaga cccucgccca gaaccugccu     1860

uacaagaaaa agaauaaaau ccuugacgag aacuucaagg acaagcaaca gcaggauuuu     1920

auuagcagaa accugaauga cacacgguau aucaccaccc ucauugcuaa auacacaaag     1980

gaauaccuga acuuccugcc ccuuucugag aaugaaaaca cgaaucucaa auccggggag     2040

aagggaagca aaauccacau ucagaccauc uccggcaugc ugacaucugu ccugagacac     2100

accugggggu uugauaagaa ggaccgcaac aaucaucucc accaugcccu ggaugcgauc     2160

auuguggccu auagcacauc cucuaucauu aaggcuuuca gcgacuuuaa gaaaaaccaa     2220

gagcuucuga aggcccgguu cuacgcuaaa gaacucaccu ccgauaauua uaagcaccag     2280

guuaaguuuu ucgagccuuu uaaaggauuc agagaaaaga uccugagcaa aauugacgag     2340

aucuuugugu cuacgccccc gcgcaagcgg gccagaggcg cgcugcauga agagacauuc     2400

uacuccgagg auaaaaugau uaagaaguau aacagcaaag aagggcuuca gaucgcccuc     2460

ucuuguggac ggguccgcaa gaucggcacc aaauacgugg agaaugacac cauggucaga     2520

guggauauuu uuaagaaaca gaacaaguuc uaugcuaucc cuauuuacgu uauggacuuc     2580

gcccugggca uccugcccaa uaaaauugug aucacaggga aggauaagaa caauaacccu     2640

aaacaauggc agaccaucga cgaauccuac gaguuuugcu ucagccucua uaagaacgac     2700

cugauucuuc ugcagaaaaa gaauaugcag gaacccgagu uugcuuacua uaacucuuuc     2760

gauaucgacc ggagcagaau uaaaaucaag aagcacgaua auaaauuuga gaaccucaca     2820

uccaaucaaa agcugcuguu cacgaacaaa gacaagggaa agaaucgcac cggcauucag     2880

aaccuuaaaa ucuuugaaaa guacaucguc acaccccugg gggauaaaau uaaggccgac     2940

uuccagccuc gggagaauau cagccucaaa accucuaaga aacacggacu guga           2994


<210>  3742
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3742
augggcaacu acacccuggg gcucgacauc ggaagcaauu ccauuggcug ggcccuggug       60

gaggaugcug ucgaagcccc cagaaucguu gacauggggg ugcggguguu cccugaggga      120

gucgaucgcg acacaaaggg ccuugaaaaa ucuaagaacg cgacgagacg ggaggcucgc      180

ggggccagac ggacccacca gcgcagacgg caacgcaaac agagacuggu gcgggcccuc      240

cgccagguug gacugcuucc gugcgauaag gcugaaguga gaagccuguu ucagacagac      300

cccuaugaga uucggaaacg cggccucgau cauagacugg agcuguacga aaucggccgg      360

gccuucuauc accucuccca aagacgcggg uuuaagucua auagaaagag cggagcucug      420

aaagaggacg gcaccgucaa gaaagaagcc ggggagcuuc agcggaagau ugaugaggcg      480

ggaugucgca caaucggcga auacuucgcc gggcuggacc cugagcagca gcggaucaga      540

ggacgcuaua ccuuucgguc cauguaccaa aaagaguucg aucuccugug ggcuaaacag      600

guggaguuuu aucccaaggu ucuuacagaa gaccugagau cuaaggugcg cgaugagauu      660

aucuucuuuc agcggccucu caagagcgcc aaagaccuga uuggcgacug cgagcuggaa      720

cccaccgaga agagaugucc ucgcgcugau ugguacgccc ggagauuccg gcuccugcag      780

gacaucaaca aucuuauuau ccaaaacccg gauggcacgg aacagaaacu gucccccgac      840

cagcgcgcgg cccuccuggg ggagcuugga caaagagaaa agcugaccuu ugauaauauu      900

cgggcuaaac ucggccugcu ggagacacag aaguucaacg ccgaguacag aaucgggcgc      960

aaggggaaaa aggucgaagc uaucaaaggc gacguguuug uguggaccau gcggggaaag     1020

agcgucuucg gaccuaaagc cugggaugcc auggcugacg agagaaaggu uucucucaau     1080

gaacuguucg uggagcuuga agaugacgau cuggcgcgca agcucgagac agaguauggc     1140

cugaccgaca aacaggugca ggccgcuaug aaggccuccc uuccccaagg cuacaugagc     1200

uuuucuagaa aagccguccu gaagcuccug ccuuucaugg aagcuggggc ccugacgcau     1260

gaggcgauug accaggcugg auaucggaaa gaagaaccca cacagaccga ugccaucgcc     1320

cgcguguccu uuccgccuga ccucagaaac cccauuguuc agaaggcucu guucgaggug     1380

cggaaggucg ugaaugcccu uguucgcgaa uacggcaaac cuacaagaau caagauugag     1440

auggcucggg augugcaagg gagcaaacgc cagagagagg aacugcacug gcggaacgaa     1500

gacaaucgca agaaaaacga ggaggucaga gaucggcuca gaaccgacau ccauaucgau     1560

gaccccucuc gcgaugacgu gauuaaguau aagcuguggc aggaauccgg aaaaaugugc     1620

cccuacaccg gccggcagau cugucaagag gcccuguuug ggccuaaucc ggaguuccag     1680

gucgaacaca uucuucccua ugauagaagc cucgacgauu ccuacaugaa ccugacacug     1740

ugcgaagugc acgagaacau ccauggcaag cgcaaucaga cgccuuuuga ggcguacgga     1800

ucuggcgccg aacgguauga gcagauuaaa caaagaauca agguucuccc cuggccuaaa     1860

cgccagaagu uccugcagca agaaaucaac cuugacagcc acauuucccg ggagcugaau     1920

gacaccagau acaucugucg cgaaacagug gcuuaucuca aacagcuggg gguccaugug     1980

cggggaacca gaggcaagau uacagccgag cugcggcacc agugggggcu ugauggaauc     2040

uuuagcgagc ucggcguucg cagagacgau gaccaccggc gccaugcugu ggaugcccug     2100

gucguggcgg ugaccgacaa cgaacaccug agacggcucg ccguuucuaa guacguccug     2160

gcugggccca gauucgagac gccguggucc gccuuucgcg augaagcucg gggcaaugug     2220

aaaagaauga acgugagcca ucgcgucugc cggaaggugu cuggagcccu ucacgaggag     2280

acaaauuaug gcccuaccgg gguuagagac caggccggac aagauauuua cgcuuaucgc     2340

aaaaagcugg aagaccucac cacacccaug guggagaaaa ucgucgaucc ugugguucgg     2400

gacauuguga gacagcgccu gcugaugaag ggcaucgauc ccgcggccgc ugacagaaag     2460

aucccuaaag acgucuggaa ggaaccccuu uacaugcggc gcaccaaaag cgauaagaug     2520

auucagauca gacgggugcg cauucaugac guuaaaaacu ccaucauugu gcucacagau     2580

gagucuggga gaccguaccg ggccgucgaa ccuggaggca aucaccacgu ggagguguuc     2640

gaguaugucc augggacgaa gaccggcaca agagaagccg agguugugac cauguuugaa     2700

gcugugcgcc ggagcagagc ccgcgcgccc gucgugcgga gagaccacgg acccggcaca     2760

cgguucguuu guucccuggc uaucaacgau auggugauga ugccugacaa gagcgggaaa     2820

auggaucugu uccgcaucca gaagaugucu gccuccaaac aagucuacuu uagacaucac     2880

accgccgcuc ggauugacag cgagcgcacg cucaucagaa agcaggccca ccuguucaau     2940

ggauauaaag ugucuguuga uccgaccggc gcgauuugcc gggcuaacga cuga           2994


<210>  3743
<211>  3000
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3743
augaagaucc ugggcuucga cauugggauc aacagcauug gaugggccuu uguggaggau       60

aaucagcucc aagacugcgg cgucagacug uucaccaaag cugaagaucc caagacaaaa      120

gagucccuug cccugccucg gcgcaacgcg agaucuaauc ggcgcagacu cgggcggcgc      180

agaagccggc ugaucgcucu uaagcacauu aucuccaaag gacugaagcu caacuaccag      240

gacuauaucg ccaaugaugg cgaacugccg aaggccuacg aggggcgccu gauuucuccc      300

uaugaacuca gauacaaagc ucugaacgag aagaucgagc cuaaagaccu ugcccggguu      360

auucugcaua ucgcuaagca ccgcggauau augaauaaaa acgaaaagaa aagcuccgau      420

aaugagaaag gcaagauucu cucugcccug aaaacgaacg cgcuuaagcu ggaaaaauac      480

cagagcgugg gcgaguauuu uuacaaggag uucuuucaga aauacagaga aaauaccaag      540

gacuucauca acauucggaa uaaggagggg uccuaugaaa acugugugcu cgccagcgau      600

cuggagaaag aacugaagcu cauccuggag aaacaaaagg aguggggaua cucuuauaau      660

gacaacuuua ucaaagaaau ucuuaagguc gcuuucuuuc agcgcccccu gaaggauuuc      720

uccuaccucg ugggcgccug cacauuuuuc gaggacgaaa agagagcuug uaagaacagc      780

uauucugccu gggaguuugu ugcgcugacc aaaaucauua augaacuuaa gucccuggag      840

aaagagagcg gggaacucgu gucuucccag aucauuaacg agauccugaa ucauauccug      900

gauaagggaa gcauuacaua caagaaauuc cgggaauaua ucaagcucca cgaguccaug      960

aaauucaagu cucugaaaua cgacaaggac aacgucgaaa gcaccaagcu gauugaguuu     1020

agaaaacugg uggaguucaa gaaagcccuc ggcgaacauu cccugagccg cgaggaacuu     1080

gaucagaucg cuacauacau uacccugauc aaggacaaug agaaacucaa gaucacgcug     1140

gagaaauauu cucugaacaa ugaacaaauu aagaaccucc uggagaucga uuuuaaugac     1200

cacauuaacc uuuccuucaa ggcccugaau cucauccugc cucugaugaa agaagggaag     1260

cgguacgaug aggcuugcaa acuucucaac cugaagacca aaagcaauaa ccagaaguuu     1320

gacuuccugc ccgccuuuug ugauucuauu uucgcccagg aacucacaaa uccuaucguu     1380

aacagagcua uuagcgagua ucgcaaggug cugaacgcgc uucugaaaaa guacggaaaa     1440

augcacaaga uccauaucga gcucgcccgg gacauuggcc uguccaaaaa gcugagagcu     1500

aagaucgaaa aagagcagaa ggaaaauuau gagaacaaua uuugggcccu uaacgaaugc     1560

gagaaauuug gccucaaggc caaugcuaaa aacauccuga agcugaagcu cuggaaagag     1620

caaaaggagu ucuguauuua cucugggaaa aagaucagca ucgaacaccu ucgcgaugaa     1680

aaagcccuug aggucgacca uauuuauccg uacucccgga gcuuugauga cucuuuccug     1740

aauaaggugc ucguguuuac caaagaaaac caggagaagc ugaauaagac acccuucgag     1800

gcguucggag cuaacgaaga gagauggucc aaaauccagg cccuggccca aaaucuuccu     1860

uacaagaaaa agaacaaaau ucucgacgag gcuuuuaagg gcaagcagca gcaggauuuc     1920

aucagccgca aucugaacga cacccgguau auuucuacgc ugaucgucaa auacacaaag     1980

gaauaucucg auuuucugcc ccuugacgag aaagaagaug uuaaucugaa guccggggag     2040

aaaggaagca agauccacgu gcaaaccauu aacggcaugc ucacauccgu gcugagacac     2100

accugggggu ucgcccagaa ggaccgcaac aaucaucugc accaugcucu ugaugccacc     2160

aucgucgcgu acucuacaaa cgccauuauc aaggcuuuua gcgacuucaa gaaagaacag     2220

gagcuccuga aggccaaacu guaugcuaag gaacucacgu ccgauucuua caagcaccag     2280

gccaaauuuu ucgagccuuu ugagggauuc cgggaacaaa uucugaauca gaucaacaaa     2340

cuuuuuguga gcaaaccccc gagaaagcgg gcgcgcggcg cccugcauaa agagaccuuc     2400

uauuccaagg acgaaaugau caagaaauac aauagccagg agggcguuga aauugcucuc     2460

aacugcggga agaucagaaa aauuggaaca aaguacgugg agaaugauac caugguccgg     2520

aucgacauuu uuaaaaagca gaacaaauuc uaugccaucc cuauuuacac aauggauuuc     2580

gcucugggcg ugcugcccaa uaagaucguu aucauuggga aggacaaaaa gggaaacccu     2640

aaacaauggc aggagaucga cgaaucuuau gaguuuuguu ucucccuuca caaggaugac     2700

cuggugcuca uucagaaaaa ggauaugcag gaacccgagu uugccuacua uaauggcuuc     2760

gacaucagca acucuuccau uugcgucgaa aagcacgaua auaaauuuga gaaccugacc     2820

gacaaucaaa gacuccuguu cacgaacgcc gaggggaacg ugaaggcuaa aaagaucggc     2880

auccagggac uucgcauuuu ugaaaaauac aucauuacac cccugggcga gaagaucaag     2940

gcggauuuca aaccucggga agacauugcc cucaagacca gcaaaaagca ugggcuguga     3000


<210>  3744
<211>  3003
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3744
augaagaucc ugggcuucga cauugggauc aacagcauug gaugggccuu uguggagaau       60

gaugaacuca aagacugcgg cgucagaauc uucaccaagg cugagaaccc caaaaauaag      120

gaaucccugg cccuuccucg gcgcaacgcg agaucuagcc ggcgcagacu gaaacggcgc      180

aaggcuagac ucauugccau caagcggauc cuggccaaag agcuuaagcu gaauuuuaaa      240

gauuacguug cugccgacgg ggaacucccg aaggcuuaug agggaucccu ggccucugug      300

uacgagcugc gcuauaaagc gcucacacag aagcuggaaa cgaaggaucu ugccagagug      360

auucugcaca ucgcuaaaca ucggggcuac augaacaaga augagaaaaa gagcaacgac      420

gccaaaaagg ggaaaauucu cuccgcucug aagaauaacg cccuuaagcu ggaaaauuau      480

caaucugucg gagaguacuu cuauaaagag uuuuuccaga aguacaaaga aaacaccaag      540

aauuuuauca aaauucgcaa cacaaaggau aauuacaaca acugugugcu cagcuccgac      600

cuggagaagg aacugaaauu cauccucgag aagcagaaag aauuuggcua uaaguacagc      660

gagggcuuca uuaaugagau ccugaaaguu gcguuuuucc agagaccccu uaaggauuuu      720

ucucaccugg ugggggccug caccuucuuc gaagaggaaa accgggcuug uaaaaauucc      780

uauagcgccu gggaguuugu cgcucucaca aaaaucauua acgaaaucaa gucucuggag      840

aaaauuuccg gagagaucgu gagcacccaa acaauuaaug aaguucuuaa ccugauccuc      900

gacaagggcu cuaucaccua caaaaaguuc cgcuccugca uuaaucugca ugagagcauc      960

uccuuuaagu cucugaaaua ugauaaggaa aacgccgaga augccaaacu cauugacuuc     1020

agaaagcugg uggaauuuaa aaaggcucuu gggguccaca gccugucccg gcaggagcuc     1080

gaucagauca gcacgcaugu gacccugauu aaagacaacg ugaaacuuaa gacaguccug     1140

gagaaguaca aucucucuaa cgaacagauc aauaaccugc uggagaucga guucaaugac     1200

uauauuaacc ucuccuuuaa ggcgcuggga augauccuuc cucugaugag agaaggcaaa     1260

cgcuacgaug aggccuguga aauuaccaac cucaagccca aaacaguuga cgagaagaag     1320

gauuuccugc cugcuuuuug cgacagcauc uucgcccacg aacugucuaa ucccguggug     1380

aaccgggcca uuagcgagua cagaaaaguc cuuaaugcuc uccugaagaa auaugauaag     1440

gugcacaaaa uccaucugga acucgcccgc gacguugggc uguccaagaa ggcgcgggag     1500

aaaauugaga aggaacaaaa agagaaccag gcugugaaug ccugggcccu uaaggaaugu     1560

gagaacaucg gacugaaggc uucugccaag aauauccuca agcugaaacu guggaaggaa     1620

cagaaagaga uuugcaucua cagcggcaac aagauuucca ucgagcaccu uaaagaugaa     1680

aaggcucucg aggucgacca uauuuauccu uacagcagau cuuuugauga cuccuucaug     1740

aauaaagugc ugguuuuuac caaggaaaac caggagaagc ugaauaaaac gccguucgaa     1800

gccuucggca agaacaucga gaaauggagc aagauccaaa cacucgcgca gaaucugccc     1860

uauaaaaaga agaacaaaau ucuugaugag aauuuuaagg acaaacagca agaagacuuc     1920

aucucucgca accugaacga uacccgguac auugccacac ucaucaccaa guauaccaaa     1980

gaguaccuga auuuucugcc ucuuaacgaa aaugagaaca caaaucucaa guccggggag     2040

aagggaagca aaauucacgu gcagacgguc aacggcaugc ugaccuccgu gcugagacac     2100

acaugggggu ucgacaagaa agaucgcaau aaccaucucc accaugcucu ggacgccauc     2160

aucgucgcuu acucuaccaa uagcauuauc aaggccuuuu ccgauuucaa aaagaaccag     2220

gaacuucuga aggcgcgguu uuaugccaaa gagcucacau cugacaauua caagcaccag     2280

gugaaauucu uugaacccuu caagggauuu agagagaaaa uucugagcaa gaucgaugaa     2340

auuuucguuu ccaagccucc ccgcaaacgg gcuagaggcg cccugcauaa ggagaccuuu     2400

uauagcgagg acgaaaugau caaaaaguac aacucuaaag aggggcuuca aaucgcucuc     2460

uccuguggac gggugcgcaa gauuggcacg aaauaugucg aaaaugauac aauggugaga     2520

guugacaucu ucaagaagca gaacaaauuc uacgccauuc cgaucuaugc cauggauuuu     2580

gcucugggca uucugccuaa uaagaucgug gucgugggga aagacaagaa caacaauccc     2640

aaacaguggc agaccauuga ugagagcuac gaguucugcu uuucucucua caagaacgac     2700

cugaucaugc uucaaaagaa agacaugcag gaagcggagu ucgccuauua caauggauuu     2760

gauaucucca ccagcucuau uuguguggaa aagcacgaca gcaaauucga gaaccugaca     2820

uccaaucaga agcuccuguu uagcaacgcu aaagaaggcu cuguuaaggu cgagaaucug     2880

gggauccaga accuuaagau uuucgaaaaa uauaucauua ccccucuggg agauaagauc     2940

aaagccgacu uucaaccccg ggagaauauc ucccucaaga caagcaaaaa gaacggccug     3000

uga                                                                   3003


<210>  3745
<211>  3003
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3745
auggccagag cucagcggcg ccugaucagc agacgggcca accgcaugaa uaagcucaga       60

cggcugcuua aacaagagaa cgugcugcag gcgaaugacu acgaugaaaa gggccucauu      120

aucgggcugc ccaaccaggc uugggagcuu cgcgcccagg gacuggacag aaaacucgaa      180

ccuaaggagu gggccgcugu ccugauuaau cugguuaaac accggggcua ucucucccaa      240

cgcaagaacg aaucucagac cgccgauaag gagcuggggg cucugcugag cggaaugaau      300

cauaaccacc agcuccugca gucccaaucu aaucaguaca gaacaccggc cgagcuugcg      360

aucaaacagu ucgccacgca ggacggccau auucggaacc aacgcggggc uuauacccac      420

acauuuaaua gacuggaucu ccagaacgaa cugaaucagc uguucgacgc ccaaacccgg      480

cucggaaacc cccaugugag cggcucccug aagacagcua ucaaugaucu ucugaugaac      540

cagaaagccg cgcucucugg cgacgccauc cuggagaugc uugggcgcug caccuuugaa      600

ccuagcgagu acaaggcugc caaaaacaca uauuccgcug agagauucgu guggcuggga      660

aagcucacca aucugcggau ucaggauaac ggccuggaaa gcgcccucuc uagagagcag      720

cgcgaacugc uucugaauga gcccuacaac aagacgaaac ucaccuauca acagguccgg      780

aagcugcuua gacugccuga aacagccuuu uucaaagggc uccgcuacgg aaaggacaau      840

gcugaguccg cgaccuuuau ggagaugaaa agcuaccacg ccauccggaa gucucuggaa      900

aacaaauccc ugaaaagcga gugggaaaag cucaaaacac agcccaagcu gcuugaucug      960

auuggcaccg cuuucucuau cuauaaaacg gacgaugaga uugccgggug ucucaagaau     1020

cagcugccug aaaacgugau cauugcccug caagagcagc uuaauuuuuc caacuucaug     1080

gagcucagcc ugaaagcucu ggaaaagcuc cugccgcuua uggagcaggg acugagauac     1140

gacgaagccu gcacacagau cuauggcaau cgccacgaga agaucgcgga caaaaccgaa     1200

aaguuucucc ccaacauucc ugcugauaaa cugcggaauc ccguugugcu gagaacacuu     1260

acccaagccc gcaaggucau caacgccauu auccggagau acggcucccc ugcucgcgug     1320

cauauugaga ccgggcggga gguuggaaaa ucuuucagcg acagagauaa gcucaagaaa     1380

cagcaggaag agaaucagga acaacggcgc agagccaucg acgaguggaa gcaguuuuuc     1440

cccaacgcug cccacgagcc gaaagcggug gauauccuga agcugcggcu cuaucagcug     1500

caacagggca aaugucuuua cuccgggcug ccuauugacg ucagacgccu ccccgaaaag     1560

ggauaugugg agaucgauca ugcccugccu uucagccgga caugggacga uucuuuuaac     1620

aauaagauuc uggugcuugc uuccgaaaac cagaauaaag gcaaccagac gcccuacgag     1680

uaccucagag gggccgacaa uagcgaaucu uggcgccugu ucgucgagaa cguucaaaau     1740

ugccaguuua gccccaacaa gaaacagaag aucauggcua aacagcugga ugagaaggga     1800

uuccucgaaa gaaaucugaa cgacacccgg uauguguccc gcuuucuuug ucaauucauu     1860

ucugagcacc uucaccucac aggcaaaggc aagaagagag uguuugccag caaugggcag     1920

aucaccgcgc ugcugcgggg acgcuggggc cuuuccaaaa acagagaaga gaaugaccgg     1980

caucacgccg ucgaugcugu gguuguggcc ugcagcacag ucgcuaugca gcagaagauc     2040

acccaauucg ugcgcuacca gaaagggaac guuuuuacgg gagaaagaau ugacaaggag     2100

acaggcgagg ugaucucuau ucauuucccu accccguggg aacacuuucg ggaggaaguc     2160

augauccgcg uguucgauga caauccccuc agaacccugc cugagaaacu gcccaaccgg     2220

ccugaagccc uccaugagua ugucaugccc cuguuugugu ccagaaugcc gacacgcaag     2280

augaccgggc agggccacaa ggagacaauu aaaagcgcca agcggcuuga ugaaaagauc     2340

ucuguucuga gagugccucu cacgcaacug aagcugaacg accuugagaa uauggucaac     2400

cgcgaacggg agcccgcucu guacgaggcg cucaaagcca gacuggaaca guucaagaau     2460

gauccugcua aggccuucgc cgagcccuuu uucaaacagg gaggccagca agugaaggcu     2520

guucgcgugg aaauugucca gaaauccggg augcuccugc ggaagaucaa cggaguggcc     2580

gacaauggca gcauggugag aguugauguc uuugagaaau ccgggaaguu cuaucuugug     2640

ccuaucuacg cguggcaagu ggcugaaggc auucugccca acuaugccgu cguggcccac     2700

aaggacgagu cugauuggau ccucauggac gagagcuacc auuuuaaauu cgcucuguac     2760

aagaaugauc ugcuugaagu ucagaccaaa aagggaggga uuaugggcua uuuugccucc     2820

cuggaccgcg cgacaggagc uaucaccauu cgggagcacg acagagauaa aaagaaaugu     2880

aaggacggcg aacaucgguc uaucggggug aagauggcca gcuccuucaa aaagcuccag     2940

gucgaugagc ugggcaaaaa cauccacgaa acacgcagca gaaagcggcc gaauauugga     3000

uga                                                                   3003


<210>  3746
<211>  3012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3746
augagaaucc ugggcuucga cauugggauc aacagcauug gaugggccuu uguggagaau       60

gaugaacuca aggacugcgg cguccggauc uucaccaaag cugagaaccc caagaauaaa      120

gaaucccugg cccuuccucg cagaaacgcg cggucuagcc gcagacggcu gaagcgcaga      180

aaagcucggc ucauugccau caagcgcauc cuggccaagg agcuuaaacu gaauuacaag      240

gauuauguug cugccgacgg ggaacucccg aaggcuuacg agggaucccu ggccucugug      300

uaugagcuga gauacaaggc gcucacacag aaccuggaaa cgaaagaucu ugcccgggug      360

auucugcaca ucgcuaagca ucgcggcuau augaauaaga acgagaaaaa gagcaaugac      420

gccaaaaagg ggaaaauucu cuccgcucug aagaacaaug cccuuaaacu ggaaaacuac      480

caaucugucg gagaguauuu uuacaaggag uucuuucaga aguacaaaaa gaauaccaaa      540

aacuucauca agauuagaaa uacaaaagau aacuauaaca auugugugcu cagcuccgac      600

cuggaaaagg agcugaagcu cauccuggaa aaacagaagg aguuuggcua caacuauagc      660

gaagauuuca uuaaugagau ccuuaaaguu gcguuuuucc agcggccccu gaaggacuuu      720

ucucaccucg ugggcgccug caccuucuuu gaggaagaga aacgcgcuug uaagaacucc      780

uacagcgccu gggaguucgu cgcucugaca aagaucauua augaaaucaa aucucuugaa      840

aagauuuccg gggagaucgu gccuacccaa acaauuaacg agguucugaa ucucauccug      900

gauaaaggaa gcaucaccua uaagaaauuc agaucuugca uuaaccugca ugaauccauc      960

agcuuuaagu cccucaagua cgacaaagag aaugccgaaa acgccaagcu gauugauuuc     1020

cggaaacuug uggaguuuaa gaaagcucug ggcguccacu cucucagcag acaggaacug     1080

gaccagaucu ccacgcauau uacccuuauc aaggacaaug ugaaacugaa gacagugcuc     1140

gagaaguaua accugagcaa ugagcagauc aacaaucugc ucgaaauuga guucaacgau     1200

uacaucaacc ugucuuuuaa agcgcuuggg augauucugc cccucaugcg cgaaggaaag     1260

cgguacgacg aggccuguga gaucgcuaau cugaaaccua agaccgucga ugagaaaaag     1320

gacuuccugc ccgccuuuug cgauuccauu uucgcccacg aacuuagcaa cccuguugug     1380

aauagagcua ucucugaaua ucgcaaggug cucaacgccc ugcugaaaaa guacggcaaa     1440

guccacaaga uucaucucga gcuggcgcgg gacguggggc uuagcaaaaa ggcuagagaa     1500

aagaucgaga aagagcaaaa ggaaaaucag gccguuaacg ccugggcucu gaaagagugu     1560

gaaaauaucg gacucaaggc cuccgcuaaa aacauucuga agcugaaacu uuggaaagag     1620

cagaaggaaa ucugcauuua uucuggcaau aaaaucagca uugagcaccu caaggaugag     1680

aaagcccugg aaguggacca uaucuacccg uauucccgca gcuuugauga cucuuucauc     1740

aacaaggucc ugguguuuac aaaagagaau caggaaaagc ucaacaagac ccccuucgag     1800

gcguuuggca aaaauauuga aaaguggucc aaaauccaaa cgcuggccca gaaccuuccu     1860

uacaagaaaa agaauaagau ucuggaugag aacuucaaag acaagcagca agaggacuuc     1920

aucagccgga accucaauga uacaagauau auugcuaccc ugaucgccaa auacacaaag     1980

gaauaccuga acuuucuucu ccugucugag aaugaaaacg cuaaucugaa auccggggag     2040

aagggaagca agauccacgu ucagaccauu uccggcaugc ucaccucugu gcugcgccac     2100

acaugggggu ucgacaaaaa ggaucggaac aaucaucuuc accaugcccu ggacgcgauc     2160

auugucgccu auagcacgaa cuccaucauu aaggcuuuuu cugauuucag aaagaaucag     2220

gagcuccuga aagcccgcuu uuacgcuaag gaacugacca gcgacaacua uaagcaccag     2280

gugaaauucu uugagcccuu caaguccuuu cgggagaaaa uccuuagcaa gaucgaugaa     2340

auuuucgucu cuaaaccucc cagaaagcgg gcccgcagag cccuccauaa ggacacauuu     2400

cacuccgaaa auaaaaucau ugauaagugu agcuacaacu cuaaagaggg acugcaaauc     2460

gcucuguccu gcggccgggu gagaaagauu gggaccaaau auguugagaa ugacacaauc     2520

gugcgcgucg auauuuucaa gaaacagaac aaguuuuacg cgaucccgau cuaugccaug     2580

gacuucgcuc ucggcauucu gccuaauaag aucgugauua ccggaaaaga uaagaacaac     2640

aaucccaaac aguggcagac gaucgacgaa agcuacgagu ucuguuuuuc ucuuuacaag     2700

aacgaccuga uucuccugca gaaaaagaau augcaagaac cugaguucgc cuauuacaac     2760

gauuuuagca ucuccacaag cucuaucugc guugaaaagc acgacaauaa auucgagaac     2820

cugaccucca aucagaaacu ucuguuuagc aacgccaaag agggcuccgu gaaggucgaa     2880

ucucucggga uucagaaucu gaaaguguuc gagaaguaua ucauuacacc ccucggagau     2940

aagaucaaag cugacuuuca accccgggaa aacauuagcc ugaagaccuc caaaaaguac     3000

ggccuuagau ga                                                         3012


<210>  3747
<211>  3012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3747
augaucgacg agagccugac cuucggcauu gaucucggga ucggauccug cggcugggcc       60

gugcugagac ggcccucugc uuuugggcgc aagggaguca uugaaggcau ggggagcugg      120

uguuucgacg uuccugagac auccaaagaa agaacgccga ccaaccagau ccggcgcucu      180

aauagacuuc ugcggcgcgu gauuagacgg cgcagaaacc ggauggccgc gauccgcaga      240

cuccugcacg cugccggacu ucugcccagc acagauuccg acgcccucaa gcggccuggc      300

caugaucccu gggagcugcg cgcuagaggc cuggacaaac cucucaagcc cguggaauuu      360

gccgucgugc uggggcacau cgcuaaacgg agaggauuca agucugccgc gaagcgcaaa      420

gccaccaaua uuagcuccga ugacaagaaa augcuuacag cucuggaggc cacccgggag      480

agacucggcc gcuaccggac aguuggggaa auguuugcua gagauccuga cuucgccagc      540

cgccggagaa accgcgaggg aaaguaugau cggaccacgg cgagagacga ucuggaacau      600

gaggugcacg cccuuuuugc ugcccaacgc cggcugggcc agggguucgc uucuccggag      660

cucgaagagg ccuuuaccgc cuccgcuuuc caucagagac ccaugcagga cagcgaacgg      720

cuggucggau uuugcccuuu cgagcgcaca gagaaaagag cggccaagcu gacccccucu      780

uuugaacggu ucagacuccu ggcucgccuu cugaaucucc ggaucacaac cccugacggc      840

gagagacccc ugacggugga ugaaauugcc cuuguuacac gcgaccuggg caagaccgcc      900

aaacucucca ucaagagagu gcggacacug auugggcugg aggauaacca acgcuucacc      960

accaucagac cggaagacga ggaucgggac auugucgcuc gcacaggagg cgccaugacg     1020

gggaccgcga cacucagaaa agcucuggga gaagcccuuu ggaccgauau gcaggagcgg     1080

ccugagcagc uggacgccau cguccaggug cucagcuuuu ucgaagcuaa ugagacaauc     1140

accgaaaagc ugcgcgagau uggccuuacg cuggccgugc ucgauguucu gcugacagcu     1200

cucgacgccg ggguguuugc gaaauucaag ggagccgcuc acaucucuac caaagccgcu     1260

agaaaccugc uuccccaccu ggaacaaggc cggcgcuacg augaggccug uaccauggcg     1320

ggcuaugacc augccgcuuc cagacucagc caccaugggc agauuguggc caagacacag     1380

uuuaaugcuc uggucaccga gaucggagaa uccauugcca acccuaucgc ccggaaggcu     1440

cugaucgagg gccuuaaaca gauuugggcg augagaaauc acugggggcu ccccggaucu     1500

auccacgugg aacuggcccg cgacguuggc aacagcauug agaagcggag agagaucgaa     1560

aaacauauug agaagaauac agcucugcgc gcccgggaaa gacgcgaggu gcacgaucuc     1620

cuggaccuug aagaugucaa cggggacacg cugcuccggu acagacugug gaaagagcaa     1680

ggcggaaagu gccuguauac cggcaaggcc auccauaucc gccagauugc ugccacagau     1740

aauuccgugc agguugacca cauccuuccc uggagccggu ucggggauga cucuuuuaac     1800

aacaaaaccc ucugucuggc guccgcuaau caacagaaga aaagaagcac accuuacgag     1860

uggcugucug gacagaccgg cgaugccugg aacgccuucg ugcagcggau ugaaacgaau     1920

aaggagcucc gcggguuuaa aaagagaaac uaucugcuua agaaugcuaa agaagccgag     1980

gaaaaguucc ggagccgcaa ccugaaugac accagauacg cggcucggcu cuuugccgag     2040

gccgucaaac ugcuguacgc uuucggagag agacaagaaa agggcgggaa ccgccgggug     2100

uuuacaagac cgggcgcccu uaccgcugcc cuccgccagg cguggggagu cgagucccug     2160

aaaaagcagg auggcaagcg gaucaaugac gauagacauc acgcccugga cgcucucaca     2220

guggccgcug uugacgaagc cgagauucag cgccugacca aaucuuucca cgaaugggag     2280

caacaggggc uuggacggcc ccugagacgc guggagccuc ccugggagag cuucagagcc     2340

gaugucgaag cuacguaucc ugaaguguuu guugcgcggc ccgagcgcag acgggcccgc     2400

ggcgaggggc augcugccac aaucagacag gugaaggaac gggagugcac cccgauuguc     2460

uucgaaagaa aagccguguc cagccucaag gaggcugacc uggaacgcau caaagaugga     2520

gagcggaacg aggccaucgu ggaagcgauu agaucuugga ucgcuacagg ccgcccugcc     2580

gacgcucccc cucggucccc cagaggggau auuaucacca agauucggcu ggccaccaca     2640

aucaaagccg cgguuccugu ccgcggcgga acggcuggca gaggggagau ggugcgggcc     2700

gacguguuua gcaagcccaa ucgcagagga aaggaugaau gguaccuugu cccgguguau     2760

ccucaccaga ucaugaaccg gaaagccugg cccaagcccc cuaugcgcuc uauuguugcu     2820

aauaaagacg aggaugaaug gaccgaggug ggcccggagc aucaauucag auuuucccuc     2880

uacccccgga gcaacaucga aauuauccgc ccuuccgggg aggucauuga aggauauuuc     2940

gugggccugc acagaaauac aggggcccug auccccaccc cuguuggccc cgacucuuac     3000

gugaucgcuu ga                                                         3012


<210>  3748
<211>  3024
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3748
augaaggugc ugggcuucga caucgggauu aacagcaucg gaugggccuu ugucgagaau       60

aacgaacuca aagauugcgg cguuagacug uucaccaagg cugagaaucc caaaacaaag      120

gaaucccuug cccugccucg gcgcaacgcg agaucuagcc gggugcgccu cagacggcgc      180

agaucccggc ugcuuucucu gaaacacauu aucagcaagg aguuugggcu ccauuacgaa      240

gacuauauug cucuggaugg aaaucugccg aaggccuaca cgggcaaaau cgaguccauc      300

uauaagcucc gcuacgaggc ccugucucag gcuauuagca aagaagaccu ugccagagug      360

auccugcaca uugcuaagca ucggggguau augaacaaaa aucaaaagac cucccaggau      420

agcgagaagg gagccauccu cucugcgauu aaagaaaacu ccaauaagau caaacacuac      480

cagacagccg gcgaguauuu cuacaaggag cuguuugaaa accuucgcga cggcaccaaa      540

gaguucaaga auauuagaaa caaagcuaug aagaaugaaa agacagggaa cauggagagc      600

accuacuaua auacaauccu ggccucugau cucgaaaaag agcugaagcu gauccucgag      660

aaacagcagg cuuaccauaa cuauuccgac accuuuauua aggaaguccu ggagaucgcc      720

uucuaccaac ggccccuuaa agauuuuagc caccuggugg gaucuuguac guucuaugaa      780

aacgagaagc gcgcgugcaa aaauuccuac agcgccuggg aauuuauugc ucucggcaaa      840

aucauuaaca cccuggaguc caucaagaaa gacacagggg agaucuucuc uaaugaaauu      900

aucacccaga uucuuaagga gguucuggau aaaggaagcc ucuccuauac aaagcugaga      960

aagaugauca accuggacga aaaagugaag uuuaaaggcc ucaaguacga uaaagagggg     1020

gucgaaaaga ccaaucugau ugaguucaag aaacuuaagg aguucaaaaa ggcccuggga     1080

gaacacaacc ucgagcggaa aacgcuggac gaaaucgcua cacauaucac ccuuacaaag     1140

gacgagaauc agcugaaaaa gcaacucgag caguacgccc ugagccagaa ccagauugau     1200

gcccuggcug aacucgacuu uaaugaucac aucaaccugu cuuucaaggc gcuuuaugag     1260

auucugccuc ucauggaaaa aggcaagaga uacgacgagg ccuguaauga acugggccug     1320

aaagcuaaga ccuccaacca aaagagcgag uuucuucccg ccuucugcga uucuaucuuu     1380

gcccaggagc ucaccaaucc uauugugaac cgcgcuauca gcgaauaucg gaagguucug     1440

aauagacugc ucaagaaaua cgggaagaug cauaaaauuc acaucgagcu ggcccgcgac     1500

gcgggacuuu ccaaggaagc ucggaacaaa aucgagaagg aacagaagga gaacaaagau     1560

auuaaugaca aggccgagaa aacauguaag gaguucggcc ugaaacccaa cgccaagaau     1620

auccucaaga uuaaacugug gagagaacaa ggggaaaucu gcgcuuauuc uggaaagaaa     1680

auuacgaucg augaccugaa ggaggauaaa gcccuugaag uggaccacau cuacccuuau     1740

agccgcuccu acgacgauag cuacaacaau aagguccucg uguuuauuaa agagaaccag     1800

cugaagcuga aucagacccc guaugaggcu uucggcucua accaggaaaa gugguccaaa     1860

auccaagugc ucgcccagaa gcugcccuac aaaaagaaaa aucggauucu ugacgagaac     1920

uuuaagaaua aggaucagau cgaguucauu agcagaaacc ugaaugacac acgcuaugcg     1980

accucucuca ucgccaaaua cacaaaggaa uaucuggagu uucugccucu uuccgaugac     2040

gaagauauca gccucaaauc cggggagaag ggaucuaaaa cccaugucca gacgaugaac     2100

ggcaugcuga caagcguucu gcggcacgcu uggggcuucu cccaaaagga cagaaauaac     2160

caucuccacc augcccugga ugcuauuauc auugccuacu cuaccaacag caucauuaag     2220

gcguuuuccg acuucaaaaa gaaucaggaa auccuuaaag ccaagcugua ugcuaaaaag     2280

cucaccagcg aggaauacaa gaaccagucu aaauucacag gguuugagaa guaccgcucc     2340

aaaauccugg agaagcugga uagcauuuuc gugucuaaac ccccuaauaa gaacaccaag     2400

ggagcccuuc acgaacagac auuuuauucc uaccaagaca uccucgagga auauaaaacg     2460

caggagggcc ugaagcgggc ucuggaaugu gggaaaguga gaaagauugg aaccaaauac     2520

gucgugaaug aucggauggu ucgccucgac aucuucaaga aagacaacaa guuuuauggc     2580

auucccaucu acacaaugga uuucgcccug ggggugcuuc cgaauaaggu cgugaccauu     2640

ggcacagaca aagauaagau caaaaaggag uggaaaacca ucgacgagac guaugaauuu     2700

ugcuucagcc uguacaaggg agaucucguu cugauucaga agaaagagau gcaggaaucu     2760

gaguuugccu acuauaacgg cuucgacauc agcuccgcua gcauuucucu ggaaaagcac     2820

gauaauaaau uugagaaccu uaccgagaau caaaagcucc uguucccuaa agcgaaggaa     2880

ggggacgugg aggucagagg aaucggcauu caggggcuga agguguuuaa aaaguacaug     2940

gucacacccc ucggagaaau ccaggagacc cggacagagg ccagacaaaa cauccagccu     3000

cgcaaaaagc auggccugcg guga                                            3024


<210>  3749
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3749
augagcgacu ccucuuacgu gcugggccuc gaucugggga ccaacagcau cggaugggcc       60

ugcgucagac uuaauaaggg caaacccgac gggcugcucg cucagggagu ucggauuuuc      120

gaggccggcc uggaaaucga ugcguauggg cgcggaacac cucuuaacac ggagagacgg      180

aaugcucgcc aaagacggcg ccugcacgac agacggaagc gccgcauucu caaagugcgg      240

cgcauccugc aggaagccgg caugcugccg gccaacaagc ccgaucauaa agacgcugag      300

uggaagaugc uccuggccag aaauccuuac cggcuucgcg cuaaggcccu ggaugaacag      360

auugaccuca aagagaucgg ccuguguauc uaucaguuug cgcacagacg gggguuccuu      420

uccaacucua agauucugaa aaaggaugac gauagaggaa aacucaagga cgccaccaag      480

gcuuuugccg aggauaugga acaaaaaggc gcucgcacau ggggagagua ccugagccau      540

cuggaccccc acgaacagcg gaucagaacc aauuauacac uccgcgagug guacaccucc      600

gaguucgacc ugauuuggga aaagcagcag ucuuuuuauc cucaugugcu uacagaugac      660

cuguacaaac gggucaagga ggcccucuuc uaccaaagac cccugaaaag ccaggcgcac      720

cuucuggggc agugcgaacu cgagccuggc cgcaagcggg ccccguuugc ucugcugaug      780

gcccaggagu ucagacuccu gcaaucccuu aaccaucugc gcgugguuag cucuaccggg      840

gaugaacggc uccugacgga cgaggaaaga aaacaggcuc uugcccugcu ccgcucccgg      900

ggaaaucaga aguuuaccac acugagaaag gugcugaacc uccggggcac caaauucaau      960

cuggagcgcg gcggggaaaa gacaauccaa ggaaacaaua ccgccgcugc gcuuggcaac     1020

cuguuugggg aggccuggga ucacuucagc ucugaacaga aaaaucagau ugucgaggac     1080

agacucacga ucggagauac agacgcucug auucggagaa ugaagaaaac cuggggccug     1140

gauuccguga cagccgagau guuuagcgaa accgagcuug aagacgggua uuguggauuc     1200

ucucgcaagg cccucgagaa gcugcugccc cuccuggaac ggggccacgc ucuuauggag     1260

gccguucagg cggcuuaccc ugauuaucaa aagaaccugc ccagaaccga cacacucccu     1320

ccccuggaga accugcgcaa uccgaucgug cagagaacgc uuuccgaacu ccggcgcguc     1380

gugaacgcca ucauuaagga guacggcaaa agcgacaaga ucagaauuga acuggcccgg     1440

gauaugaaac agaccgcuaa gaaucgcgag aaguccuggg acagaaugcg ggugcgcgag     1500

agacagcgga aagaagccgc uaagaaaauc cuggaggaaa cagggaaccc ugcccccaga     1560

agacaugaga uugaaaaggu ccuccuggcg gaggagugca auugggaaug uccuuauacc     1620

ggaaaacgga ucugcaugaa cacacuucug ggcgaugcuc cccaauucga caucgagcac     1680

auuauccccu uuucucgcag cuucgauaau uccuuuagca acaagacccu cugugacguu     1740

aauuacaaca gaaaugugaa ggggaacaaa acgccuuaug aaucugccuc cgagacagaa     1800

cacgaggaga uggugugccg ggucaagaga uucaccggag auuacaaaga aauuaagcug     1860

gaccgcuuua auacccggga gacagauagc agagccgacu ucaucgaaga guuuucuacc     1920

agccagcuga acgauacacg cuacgcuucc cggcuugccg gcgaauaucu cgggaaacug     1980

uacggcucug aguggagaaa gcauauucag aucgcgaaag gaggcacgac caaguaucug     2040

cgcgacgccu gggaucucaa ucggauccug agcgacgggg gaacaaaguc cagagacgau     2100

caccggcauc acgcugugga cgccguugug gucgcucuua ccacaccgca gaccgugaaa     2160

gcccugagcg augccaacca acgcgcuccc gagcauagac gguacgcguu cucugacaug     2220

ccucuccccu gggaaacguu ucgccaggau guugagcagg ccauuaacuc caucaccgug     2280

agccacaagg ucuguagaaa agcuaauggc cagcugcaca aggaaacauu cuaugggcug     2340

auuaaagacc ggaagaccgg agauacaaga accgugcuuc gcaagccucu ccaugagcug     2400

acggacaaag agaucaagcg ggccgaaauu gucgauucuu ccaucagaaa acugauugag     2460

caaaagcuca aagaccugga ucagcccccg aagacagccu uuaaggaccg caacaaucac     2520

ccuuucauca ucgauaaaaa cggcaauaag cgguuuauuc auaaagugag aguuuaccag     2580

gacgcuaagc ccgugaaaau cgccgaaaac cgccacguca aacuuggggg caaucaccau     2640

cuggagauuu ucgaagugau cgacaagaaa ggaaacccua ccuggaagug cgagguuauu     2700

agccucuucg auacacggca aagagugaaa gagaagcgcc ccgucaucgu gagacaggac     2760

cagaauggca acccucugaa auuuucccug ucuaucgggg aagcggugca gcuugauugg     2820

aauggaggcc gggagaccgc uguuguccaa aagaugagca aaacccccaa cacaguggaa     2880

uauguguucc gcaagcacaa ugacgccaga aaggccgcug auauuccgua caaagagacg     2940

aucaccguca aguccgacuc ugcccuguuu gcgagcaacc uccagaaaau ugugaucaca     3000

ccuaccgggc ugauucggcg cgcuaaugau uga                                  3033


<210>  3750
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3750
augaagaaca gaaaugugau cggcauugac gucgggacca acagcaucgg augggcccug       60

guugaggaaa augagaacgg ccucccccag ucccugaaag cugccggggu gcggauuuuc      120

caagaagcgg uggaggcuaa gacacgcacg ccuaaaaauc acaagagacg gcaggcccgc      180

cuugccagac ggguccuggc ucgcagagcc cggcgcaaac agagacucca gaaccugcuu      240

cugaagcaug gacuccugcc gggcggggug accgaucccg uucaacggga agcugccuuu      300

acaacccugg gagacccuua ccagcuccgc gcgaagggcc ugaaucuucc ccugggccuc      360

cacgaguucg ggagagugcu guuucagcuu ggagcccggc gcggcuuccu gucuaacaga      420

aaaacacggu augggcaucu caauaagcug gaugagcugg cugaccucau cgaagccgag      480

gaugcugccc cuaaaaccgg agacgcggaa gagggcauua ucagaaagga gaucggggaa      540

cugcgccagg ccaugagcgc ugccggaucc cggacacuug gcgaguaccu ggcuggccuc      600

gccaaagaag agagaaagcg cugccggcac accgaaagag ccaugcugga ggaggaauuu      660

gagcgccuuu ggauggucca agcucgguuc caucccgaac ugucugauga gcucaaggug      720

cagguucaca cggugaucuu uuuccagaga ccucugaaaa gcaaccgcgc gucccugggg      780

aaguguucuc ucgaaccgag ccggagaaaa gccgcucgcg ccuggcccga ggcccagcgg      840

uuuagacugc uucaagaccu gaauaaccuc gagauucugg auccucggac cggagaacuu      900

cgcagacugc gggacacaga gagagcuauu cuccugaaug cccuggaaca gcagaagucc      960

augaccugga acgcgguccg caaaaagcuc ggccugcacc aaggggagaa auucaaucuu     1020

gaaacaggag cuaaggccga gaccggccug aaggggcucc agacgcuggc cagccugcau     1080

gcuauccuuc agggaugguu ugaugagcuc ggcgacaaac gggaucugcu gauugaagac     1140

cucaucacaa uugaggacuc ugccggccug uauagacgcc uuagagaaca cuggggguuc     1200

gaucgggaga ccgcuuuucg ccuggccaca cucgagcugg aacccggaua cgcgucccug     1260

agccuuaagg ccaucagaaa acuccugccu cauaugggcg cugggcaccg guauucugag     1320

gcccgccagc aagugggaua cggcuaugaa accgggcaga gagaccugca ggagcggcuc     1380

ggcgaacccc cggauccucg caaccccgug gucgacaagg cucugcacga gcuuagacgg     1440

guuaucaacg cccugauuca ucgcuucgga agaccugaug uggugcgggu cgagcucgcg     1500

agagaccuga aacuggccaa gcgcgauaag gaacggcuug agaaacagaa uagagaaaac     1560

caacgccuca aucggaaggc ugaggccgcu cuggccgaac ugggccucgg gcagcccucc     1620

agagacgauc ugaucaaaua ccgccuuugg gaggagagca agggacugug ccccuacacc     1680

ggccgggaaa uugggcucuc uacacuguuc uccccugagg uggacguuga acacaucauu     1740

ccguauagca gaugucugga ugacuccuac augaacaaaa cgcuuugcau gaccgaggaa     1800

aaucgcaugc agaagggaaa ccggacaccc ugggaggccu auggcgggga cccugcuaga     1860

uacgagcaga uccuccaaug ugcgcgguau uuucccaugg ccaagcgcaa aagauuccug     1920

gaacagggcu cugauuuugu ggaccaguuc auccagcggc aacugaauga uacccgcuac     1980

auugcuaagc ucgccaaaga guaucuggcc gcugucugca caaacgugca gguuaccaag     2040

ggaggcacga cagccaugcu uagacaucac ugggggcuga aucggcuccu gggagaagac     2100

gauggccaga aagagagagg ggaccaucgc caccacgcgc uggaugcugc cgugaucgcc     2160

gucaccagcc ggucccuuua ccaaagacug gcucgcauug cccaucagcg gggacacgcg     2220

ccuagccaga gaggcuuuua caucgauccc ccguggcagg gguaucaagc ugaccucgcc     2280

cagacccugg ccggcauugu ggucucucau gcuacaaacc gcaagcucac cggagcccug     2340

cacgaggaaa cagcuuacgg ccacaucccu cauguggggu ucguuuaucg gaagacgcuu     2400

aauaaagacu uuaagcuggg agauaucgag gccauuuacg accccucccu ccaggaacug     2460

gugaaagcgc accuggagca ucacggcaac gauccuaaga gagccuucgc ugaagggauc     2520

caguguuauc auaaagacgg acacaccccc auuaagcgcg ucagaauccg ggccaaacaa     2580

gugagcgaga agggcacauu uucuauuagc gaccgcgcug ggagagccua caagcaccau     2640

aaaaccggcu ccaaucacca uguugagauc cuucggcacc gcgccacagg aaagauugaa     2700

ccuguguauc ugaccacgaa agaggcuacc gaaagagcgc ggagaggcgg ggagcccguc     2760

auccagcgcg aucauggacc ggaaugggag uucaucauuu cucucugcau caacgacaug     2820

gugcggcugg gcgaaggggg acagguggcc uacuuuagag uucagaagau ugaggcuaca     2880

aauaaccgcg ucacccuccg gagccaccug gccgccacac uugaugacga ggauaugaaa     2940

gugaccacgu ccgugccuaa gcuggcuguc augcccggcu uccaaaaggu gcucguuaca     3000

gccgugggcg aaguccugga gcugcacgac uga                                  3033


<210>  3751
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3751
augaagagaa uggccgcugu gggccugaug cccgccgacc cuaccgaggc gaaagcucuc       60

gaacugcuug auccguacgc ccugcgggcc gcugggcucg accaggcccu gccccuuaca      120

cacuucggac gcgcucuguu ucaucucaac caaagacggg gcuucaagag caaucgcaaa      180

acggauaagg gggacaacga guccggaaaa aucaaggaug ccaccaagag acuggacgaa      240

gagauggcga uuaaaaaugc ccggacauau ggcgaauuuc ugcacaugcg cagaaccucu      300

gcuacagauc cuaagaacgu ccccaccguu cggacacgcc ucagcaucgc cagacgggac      360

aaugcugaga aagaggaagc cggguacgau uucuauccug accgcagaca ucuggcggag      420

gaauuugaua agcuguggaa agcccaggcu ggauacuccc ccgacgugcu gaccgaugag      480

cuccgggacg agauuucugu gaucauuuuc caccagcgcc cucugaagac gccggaaguc      540

ggccuuugcc uguuuagcgg cucccauggg gugcccaaga aagacaagag aaucccuucu      600

gcccaccccc ucaaccagcg gagaauccug uucgagacca uuaauaaccu gcgcguugcu      660

gcccggggag aaccugccag aggccucaca cgcgaugagc gggacgcuau cgugcacgcg      720

cuggauaaua aagcccauac caagagccuu uccgggauga gcaugaaacu gaaggcucuc      780

ggaaaaguca uuaagcugag acccgaacaa ucuuuuacac uucagaccgc caaccgcgag      840

acgaucgccu gugacccggu gcgggcuucc cugagccacc cugauagauu cggcccccgc      900

uggucuaugc ucgacgccga ugcgcagugg gacguugugc agcggauuag agcuguccaa      960

uccgaggccg aacaugaugc ccuggugaag uggcugaugg cuacacacgg gcucgaccgc     1020

gaucacgccg agagcaccgc uaaagccccu cugcccgaag gauauggccg gcuuggccug     1080

acagcgacca agagaauccu cgccgcucug gaggccgacg ugauucccua cucugacgcu     1140

gucaaagccu gcggguggca ucacuccgau ggacggaccg gcgaaguucu uacagagcug     1200

ccuuauuacg ggcagauccu cgaccgccau gugaucccgg gaacguacaa ugagagcgau     1260

gacgauguga ccagauaugg ccggauuaca aaccccaccg uccacaucgg gcugaaucag     1320

cugagacgcc ucgugaacaa gauuaucaca guuuacggaa aaccugacga aauugugguc     1380

gagcuggcgc gggaucuuaa gcuguccgaa gaccagaaga gagaugugca acgcgacauc     1440

aaaaagaaua ccgaugccgc uaucgccaga ggcgcuaaac ucucugagau gggcguuccc     1500

gacacggggg ccaaccggau ggugcugcgc cugugggaaa gccuuggaga ugacgucaug     1560

acaagaaacu guccuuauuc cggcaagcgg auuagcgcca ccaugcucuu ugacgggucu     1620

ugcgaugugg accauauccu gcccuacucc cgcacccugg augacggauu cgcuaauaga     1680

acacucuguc ugaaagaguu uaaccggcag aagaccgaua agacaccgug ggaggcgugg     1740

ggcgacacgc cugccugggc uaccauugaa gccaaucuua aaaaccugcc cgccaauaag     1800

gcuuggcgcu ucgccccuga ugcgauggag agauucgaag gggagaacga cuuuacaagc     1860

cgggcucuca aagaugccca guaucugucu cgcaucgcca gaaccuaccu ggacgcucuu     1920

uaugauggcg ccgacggaaa gagccacguc ugggugguuc ccggccggcu cacagagaug     1980

cugagacgcc acugggggcu uaauucccuc cugccugaua aagacggagc ggugaaggcu     2040

aagaaccgga ccgaccauag acaccaugcc auugaugccg cugucguggc cgcuacggac     2100

cgcucucuua uccaacggau cagcaaaaug gccagaaagg augaaguuaa uggcgcggag     2160

gaaguggccc gcuccgugcc cccgccuugg gagggguucc ggaccgacau ugaaacacag     2220

cuggauagaa ucauuaucag ccaccgcgcu gaccacggac ggauugaucc cgccgcuaga     2280

aaagacggcc augauucuac cuccgggcag cuccacaacg acacagccua cggccuggga     2340

gagggcaaua gcgucguguc ucggaagccc cugcuuuccc ucaaaagcaa cgauaucgag     2400

guuaccacga agggggccaa uauucgcgac gucgaccugc agagacaucu ggaacggauc     2460

acacgcggac ucgauggcaa agcucuggag gcggcccuug cugaauuugc cgccaccgcu     2520

aagcugccug acgggacaga uaacccguac uauggacuca gacgggugag acuggaggaa     2580

acccugcaac agggcgcccg cguggagguc aaggaccgga augggaaacc cuucaaggcg     2640

uacaaaggcg auuccaacca cugcuaugag aucuggagaa ugccugacgg aaaggugguu     2700

ccccagguga uuaccacaua cgaagcucau cagaucgaug ucgagaaaaa gccucacccc     2760

gccgccaagc gccuucuccg gauuuuuaaa agagacaugg uggcuaucga acaagaugac     2820

aaggccauua ucggcuaugu ucagucuaug cacguggcga acgggcuguu caucgcuccu     2880

cauacggagg ccaaugccga ugcucgcagc cgggacaaau ccgauucuuu uagacugauu     2940

cagaugagcg ccggaccccu cgucaaggcu aacgcccgca gaguguacgu ugacgaaaug     3000

ggccggcugc gcgacccggg ggcgccuucc uga                                  3033


<210>  3752
<211>  3039
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3752
auggccaaca ugagauaccg gcuggcucuc gaccugggca gcaccucccu ugggugggcc       60

auggugcgcc uggaugcgca gaaugagaca ugcgcuguca ucaaggccgg aguuagaauu      120

uucucugacg gccggaaccc caaagauggg agcucccucg ccgugucucg cagagaagcu      180

cgggccaugc gcagacggcg cgacagacug cuuaagcgga aaacgcgcau gauggcuacc      240

cugaugaagc acggauuuuu cccucaagau gaggccgcga gacaggcccu cgugaauaaa      300

gaaccguuug cucugcgggc caagggccug gaccaugcuc uccugcccga ggaguucgcc      360

cgcgcgcuuu uucacaucaa ccagagacgg ggguucaaga gcaauagaaa aacagauaag      420

aaagagaccg acuccucugc ccugaagaaa gcuauuggac agcuccgcga ggccaugcaa      480

gcuagcggcu gucggacagu cggcgaaugg cugugcgcca gacaucagca ggggcuuucc      540

gugcgcgccc gguauagaga gaaccgcauc accaaggaug acggaaagac aaaaauugaa      600

aagagcuacg aucuguauau cgaccgggcu augaucgagg cggaguuuga ugcccucugg      660

gcuaaacagu cugaacugaa uccugagcug uuccacgaaa ccgccagagc cgagcucaag      720

gacugucugc uucaucaacg cccccugaaa ccuguuaagc ccggccggug cacgcuccug      780

ccugacgaag agagagcucc gcuugcccug cccucccagc agcgcuuucg gauuuaccag      840

gaggugaaca aucucagacu gcugcgggaa gggcucaacg aggaaccucu gacccuuaca      900

caacgcgauc agcugaucca ggcgcucgag accaauccca aaagauccuu cacacaaauu      960

cagaagcugc uuggauuuuc uggccaguuc aaccugcagg acgauaagcg gaccgaacuc     1020

aaagggaaua gcacgagcgc uaugcugucu aagaaagagu uuuucggauc ccaauggcug     1080

agcuucgacg agacacacca ggaugaaauc gucaugcagc ucguguccga ggaaaacgag     1140

gccagacuga uugccuggcu ucaggcugaa accggcguug acgagaucac agccgagcgc     1200

auugcuaaua ccccucuggc cgaaggcuau gggucucuca gcgcgaaggc ccuggagaaa     1260

auccugcccg cccuucgggc ugaugugauc accuacgaca aggcuguccu cgccgcggga     1320

uuugaucacc auucccacau uagccaugcc gcuacaggcg aaauccugcc ggaccugccu     1380

uauuacgggc aauaccucca gagacacgug ggauucggca cggggaagcc cgaggauccu     1440

gccgagaaac gcuauggaaa gauugcuaac cccaccgugc acaucggccu gaaccagguc     1500

agaacaguug ugaaugcccu uauuaaacgg uacggccauc ccucugaagu ggucguggag     1560

cuggcccgcg accucaagca gucccaagaa cagagaaaag aggaacaggc ucagcaagcg     1620

aagaaccagc ggcgcaauga gagaaugcgg caggacaucg ccgggcugcu gaacaccagc     1680

acagagcgcg uuagagcucu ugaucuccaa aagaugaucc ugugggaaga acugucuuuu     1740

aauguggccg accggcgcug uccuuauagc ggaguccaga uuuccgccca gaugcuuuuc     1800

ucugaucagg uggaaaucga gcacauucug ccguuuagcc aaacccuuga cgauucccug     1860

aacaauaaaa cgguugcuau gagacaggcc aaccggauca agggcaauag aacacccgcg     1920

gaagcugccg ccgacuucgc ugcccagggg uggccugugg aggauaugcu ccaucgcgcg     1980

agccugaugc ccaaaggaaa gcgguacaga uuugcucagg acggcauggc ccaauggcug     2040

cgcgaggaua aaggguuccu ugcccgggcu cucaacgaca ccagacaccu gucucgcguc     2100

gcccgggaau aucugucccu cgugugcccu cagggcacca gagucauucc cggacgcaug     2160

acagcuaugc ugcgggccaa guuuggccuu aaugauaucc uggggcucaa cggagagaag     2220

aauagaaacg accaucggca ccacgcggug gaugccugug uuaucgcugu gaccgaccag     2280

agcaugcugc agaaauucgc caaggcuucu gccuccgccc gcgaaaaaca acugaauaag     2340

cucgucgaga gcaugccgcu cccuugggac uccuacagag aucaugugca gcgggcuguu     2400

gaacgcauuu gggugucuca caaacccgac cauggccacg agggggcgau gcacaacgau     2460

acagccuaug gacugcuggg cgggggccau guccagguga gaaagcggau ggacggagug     2520

agaacgcgcg agaccgcugc ccucaaaguu aucgaauuug ccgaucggca agcucacccu     2580

agacauggcc ugcuucccaa cggggagccu augcccuaca agggauacaa gggcgacagc     2640

aauuauugca uggaaauugu ccgcaacgau aaagggaagu gggagggaga gguggugucc     2700

acauucgaag ccuaccagcu gguccggcag cacggccucg cgagacugcg ccauccggcu     2760

cugucuguga gcgggcggcc ucuuguuaug agacugcucc gcaaugacac ccugagagug     2820

auccacaacg gcgagcagcg gacaguccgc auuuguaaaa ugaccgccga aggagugauc     2880

gcccucgcug auacgaccga guccaauguu gacgccagaa cacggaccaa ggaucugagc     2940

uauauuuuua aaacagcggg cucucuucaa aaguccaagg ggcgcagagu ggcuaucagc     3000

cccucuggag aaaugaaaga ccccggcuuc uucgauuga                            3039


<210>  3753
<211>  3051
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3753
auggaggugu ucgucgacgu uagcgccaga cagcugggca ucgaucucgg gauuggaacc       60

ugcggcuggg cucugcuugc cgaagacucc cugaucgcgc ucgggacacg gacguuugau      120

gugcccgaga ccgacaagga acgcauuccu acaaaccaaa ucagacggca ggcucgcgga      180

augagacggg ugauugaucg cagacggcag cgcaugaaug ccgucagacg gcugcuugcc      240

gaccacggcc ugcucgcuga ugggggaaaa cugggccugg ccaccgugcg ccucgacccg      300

uggcaggcua gagccgaggg ccuggaucgg cuucugucug cgggggaacu cgccguugcu      360

cugggacaua ucgccaagca ccgcggcuuc agaagcaacu ccaaacggga cagaggggcu      420

aaugccccca aggagucuag cgcgaugcuu ggagccaucg cucaaacagg cgagcgccug      480

cagcauuggc ggaccguggg ggaaauguuu uggaaagaug aguucuacca gacaagacgc      540

cggaacagag acggagauua uucccgcacc guccagcggc ucgaccugga aacggaggug      600

cacagacugc ucgccgcuca acgccggcug ggcaauccuc uugccaccuu ugaccuggag      660

cagucuuuca uugauguugc cuuuuuccag agaccccucg cugacagcga ugacaaggug      720

ggcccuuguc ccuuugaacc uuccgagaag cgcgcggccc ggcauagccc gucuuucgaa      780

agauuucggc ugcuuucccg ccuggcugcc cucagacuga gcucuggggc cggagagcgg      840

ccccuguccg augcugaguu cgccgcggcu ugcgccgacu uuggcagacu ccagggguuc      900

agcuucaaac gccugcggca acaccuuaag cuggauccuu cuaucagauu ugccggaauu      960

gcucccgacg aggagggcaa acgcgauguc guggccagau ccggggaagc uacaccugga     1020

agcgccgcgc uccggcacug ucugggcgac accguguggg auaacaugcu uggcacacgc     1080

cccgcccugc ucgacgcuau cgccgcuauu cugaccuuca gagacgaugc cgaccggauc     1140

gcggaggggc uggccggacu cggcauugag gaaccgaucc uggaugcucu uauggacggg     1200

guccgcaaug gauccuuugg caaguucaaa gggccuggcc auaucucugc caaggcuugc     1260

agagcccugc ucccccaccu ggcccgggga cgcguuuaca gcgaugcuug ugcggacgcc     1320

ggcuaugauc auuccagaca gacggaaaca aaccuggacg auauucggaa uccuguggcu     1380

cgcaaggccc uuggggaggc ccucaaacag aucaagauua ucauucacac ccacggaaga     1440

cccacagcua uccaugugga gauggcccgg gacaucggca aaagcgcgga ugaaagagac     1500

gagauuaaga aagggaucga uaagcgcaac ucugaaaaag accgggcuag acaacaguuu     1560

cacgagaugg ucggaacccc cgccgccggc gcugaagacc ugcugcgcuu cgagcucugg     1620

aaggagcaga auggguggug ccuguacacc ggccgggaaa uuccuaucga ugcccuugug     1680

uccagcgaca accgcauuca gguugaucau auccugccgu ggucuagaag cggagacgau     1740

uccuauauca auaagacacu cuguuuuacg gaggcgaacc aacacaaaaa gggcaugacc     1800

cccugggaau ggcugcauga cgauccugag cggugggaga aauucgcuag aacaguggaa     1860

gggugcaaga ccaugaaagg acgcaagaag cggaaucugc uucucagaga cucuagccgg     1920

cuggagaaag aauuucgcuc cagaaaccug aacgauacac gguacgccug ucgccuccug     1980

cuuggcauuc ugaagcacac cuaucccgac aucgucgugg ccgcuagacc ugggccccuc     2040

acggaucggc ugagacgcgc cuggggacug cagagccuua aaaagcacga ggacggcaca     2100

cggaaagaug acgacagaca ucacgcucuc gaugccgcgg uuguggccgc uaccucucag     2160

uccaugcugc agcgccugac cacacucuuc caaaaggccg aacgggaagg gggcagacgc     2220

gacuuugucg gacuggagcc gccuuggccc ggcuuccggg cugaucuuga agccaagcug     2280

gagggggugg ucgugagcag agccgaacgc agacgggcuc gcggagcggg ccauaaagac     2340

accauuagag ccaucgggga gggagaugcu ggcccuguug uguacgaacg gaagcccguc     2400

gccgcccuca cagagaaaga ccuggaucgc gugaaggacc cugagagaaa uaaaguucug     2460

auugaaaacc uucggcagug gaucgcugcc gggaagccca aggauccgga caaagcgccu     2520

agaucuccca agggcgaucc caugacgaaa gugaagcucg ucaccacaga cgacgugcgc     2580

auucuggugc ggggaggcac cgcugauaga ggggacaugg cccgcguuga ugucuuucgg     2640

caggccgacc cuaaaggaag auggcgguuc uuccuggugc cgguguaccc ccaccagguc     2700

uuugauccua cagacuggcc ccaaccuccc aaucgcgcug ugguugccua uaagccugag     2760

aaagaaugga ccgugauggg ccccgaucac gaguuccucu ggucccugua cccguuuagc     2820

uggaucgcgg ucgaaaaggc ugacgggacg agaaucgagg gauauuuccg gggcaugcau     2880

cgcuccaccg gcgccauuuc uaucagcccu caccauucca agucucuugc cagcacaggg     2940

auuggagcua aaacccuggc cuccuuuaga aaguucgcua uggaucggau gggcaaccuc     3000

agcgagaucg aacgcgaggu gagaacaugg cacgggguug ccugcaccug a              3051


<210>  3754
<211>  3054
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3754
augagagugg acagccuggg ccggugccuc uccgaugagg aaugggcccg cgucaucuac       60

caccugugua agcauagagg guuccacugg auuucucggg cugaggccaa acagaccgaa      120

ggagacagca aguccgaggg cgggaaaguu aagcaaggac uugcgggcac agcuaaacug      180

auggaugaaa agggguaucg cucugccgcc gagauggugc ucgcugaguu ucccgaagcc      240

cagagaaaca agcagggaga guaccggaaa gcucugagcc gcgaacuucu ggccaaggag      300

cucgcgcugc uguucgccag acagcgggug uuuggcaauc gccaugcuac ggacacccuc      360

gagacagcca uccugggcaa cuccgauaaa aagucugggc uuuucuggca acagaaaccu      420

gcucuggccg gagcggaccu ccugaagaug cuugggaccu gcacauuuga aaagaaugag      480

uauagagccc cgaaagcuag cuucaccgcc gaacggcacg ucuggcugac acgccucaac      540

aaucugagaa uugugaccga ugggaucacg cggcagcuga ccgagcagga acgccaacuc      600

guucugcccc uuccuuacag acaggcugga gaccugacau auaagcagcu cggcauggcc      660

cugaccaaag ccgggcuucu ggaggcugga uguuuuaagu ucauuggccu cgcguacccc      720

acagaggccc agaaagcuga aggcaagacc aaagauccug aguccgccac gcuggugaag      780

auccccgccu ggcaagaacu gcggcagaca cucaacaagg cuagccugaa ugacgagugg      840

gaaaaacuuu cuggggaugc ccugugcgga cagccugaga ucuuugacaa gauugcgugg      900

auccucuccg ucuauaaaga ugacgacgag gugagaaccg aacuggcuaa acuuguucug      960

ccgaaccccg agggccucac agaagcccug cugaauauuc gcuucgauaa guuucaugcc     1020

cucagccuga aggcccuucg ggcuaucgug ccucacaugg agaaggggcu gagauacgac     1080

gaggcuugug uccaagccgg auaccaccau ucucagcucc acaaaauugg cgagggggag     1140

cauaaguauc ugcccccucu guacgaugga cgcgacggcg auggccggau gaucuucgcg     1200

gaagacgccg auauucccag aaacccggug gugcuucgcu cccucaauca ggcucggaaa     1260

gucguuaacg ccaucaucag acgcuauggg agcccuucug cugugcacau ugagauggcc     1320

cgggaccugu ccagacccuu ugaugaacgc aauaagauca aaaaggacca ggaugaguac     1380

caagcgcgga accagagaga ccgggagcag uucauugaac uguucggaca cccucccaag     1440

ggccucgagu uugaaaaaug gcgccuguau agagagcaga augggcaaug cgccuacagc     1500

cagaagcccc uugcuuccuc uggagacgug gccgaaaucu uccuggaugg cagcaccgag     1560

auugaccaug cucucccuua uucccggagc uuugaugacu cuaaaaacaa caagguccug     1620

gugcugacca gagagaaucg cgauaaaggg aaccggacac cguacgaaua ccuuggcgga     1680

gccgaaaauu ccgaaagaug gcagcgcuuc uuuagcuucg uugagucuaa caaggccuau     1740

agacucgcua agcggacgcg ccugcugaga aaagacuuug gcgcgaagga aagcgaggag     1800

uuccgggaac gcaaucucaa cgauaccaga uacguguguc gguuuuucaa aaauuauguc     1860

gagcagcacc ugaaacuugc cgaagggucc gaggcuaagc gcugcguggu ugugucugga     1920

caacugacaa acuuucucag aacccggugg ggccugcuga agauccgcag cgacuccgau     1980

agacaucacg cccuugacgc cgcugucgug gccgcgugua gccauucuau gguccagcgg     2040

cucuccaauu acagcagaac acgcgaacug gaacaggugc gggauggguu cguuaacauc     2100

gagaccggag aaauugugaa ucccgcuaug caccagcaac ugagagagca cuuuccugac     2160

cccuggccuu auuuccgcca ugaacucgag gcccggcuga aaaucgauuc ucccgcccuu     2220

cugagagagg aaauggcucg ccucuccagc uacacggagg aggagcuggc cgcgcugaag     2280

ccgcuuuucg ucucccgggc uccucagaga cgcaacggcg gggccgccca caaggacaca     2340

auuuauggcc agcccgaaaa acucaagaaa aauggaucug ugagccaaaa gguugcucug     2400

gccucccugg cucucaaaga caucgaucug cuugccgacc cucaucggaa cgcgaagcug     2460

uacggcgcca uuagagagcg gcucgaggcu cacgggggaa agggcgauaa agccuuuccc     2520

gcuaccaauc cucugcgcaa gcccgacaaa gaagggaacc cgaccggacc uaucgugaga     2580

acagucacca aggugaucga uaaacugucu ggcauucccg ugcggggcgg caucgccaag     2640

aacgacacaa ugcuucgcgu ugaugucuuc acgaaggccg gaagauuuca ccuggugccc     2700

guguacgucc aucaccgggu gaccggccuc ccuaaucagg cuauuacaag agggaaagac     2760

gagaaccagu gggcgaucau ugaugacacc uucgaauggc gcuuuagccu guauccgaau     2820

gaucucguuc agauccggcu gaaggacgag acacaucuug gauacuaugc cuccaccgau     2880

agaagcacgu cuuccauuag caucugggcu cacgaccgca accaaucugu gggcaaagac     2940

gggcugaucc gguccauugg agucaccaca gccaaggaua ucgaaaaauu ccauguggac     3000

acccucggca auguuuaccc cgccccuccc gagaagagac gcgggcuggc uuga           3054


<210>  3755
<211>  3066
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3755
augagacggc gcagagaccg guucaagcag cgccaagccg cucugcucaa acaccuggcc       60

ggcguggggc uuuuucccgc ggaugaggcu gccagacggg aacuggccgg acucgacccu      120

uacgagcugc gcgcuagagc ccuucaugaa gcucugccgc ucaaccacau cggccgggcc      180

cuguuccauc ugaaucagcg cagaggguuu aagagcaacc ggaaaaccga ucgcggaaag      240

gcggacgaug agggcaaaau uagacucggg gucgcccggc ugcaggaagc uaugcacgag      300

aagggauccg ccacacuugg cgaguuccug cauggccgca gagcugccgc gacggacccc      360

aaucagaucc cuucuguucg gaccagacuc cgcgccgaaa gcggggaggg agcucggggc      420

gauggguaug acuuuuaccc cgauagacgc augcuggaag acgaguucga ggccauuugg      480

gaaucccaac aggugcacca cccugcugug cuuacagaug agaucaagca gcgguuuuuc      540

gaagucaucu uuuaucagag accccugaaa caaccugaga uuggaaccug cacacucgug      600

gccggcgaac gccggcugcc gaaggcccau ccccuguucc agaaaagacg ccuccuggag      660

gagcuuaacg cucugcggau cguuuggccu ggggugcagg cggaacuccu gucuagagag      720

cagcgcgacc uugccuaccu gaagcucaaa gauaagcggg cugucagauu ugaaagccug      780

cggaaggccc ugaaacucga ccccgacgcc cgcuucaaua aggaguccga aaacagaucu      840

gagcugaaag gagaugaggu ggcugccgaa cuuggcgcua agagccgguu uggcgggaga      900

uggggacaca ccgcgccugc cgaccaaugg gagauuguuu cccgcaucca ggcccuggaa      960

agcgaugacg augaggcuca guucugugug uggcucucug aacgguaugg ccuggacucc     1020

gagcaagcca gagcugucgc cgcggcccgc cuuccccagg gguucggaag auuuggccug     1080

acagcuacca cgcggcucau uaaagcccug caggaugagg ugaucgugua cagcgaagcu     1140

guccagaaga ccgggcuggg acaucacucu gacuuccgca auggcgaggu ucucgccgaa     1200

cugccguauu acggcauugc gcuugagaga cacaucaugc cugggacagg agaucccggc     1260

gacuccgaug agaugcgggu ggggcgcgug accaacccua caguccauau uggacugaau     1320

caacucagac gggugguuaa ccgccugauc aaaagauacg gcccuccccg gcagaucgcc     1380

guggaacugg cucgcgagcu uaagcucgac gaccagcaca agagagaaau uaaugggcgg     1440

aacagacaga aucgcgaggc cgcugaacgg agaagccaaa aacuggucga uaucggcauu     1500

gccgacaagg gagccaaccg cgcucugcuc aaacuguggg aggagcuuaa ucccgauaac     1560

gcgcuggacc ggagaugcgu guauaccggc cgccagaucu cuauugaaau gcucuuuucc     1620

ggggccguug agguggauca uauccugccg uucgcugcca cgcuggacga uagcaacgcc     1680

aauaagcugc ucugucugcg ggaagcuaac agaaauaaac gccggagauc ccccuuugag     1740

gccuucggac agggcgcgga cugggaagag aucgcugccc gggccgcucg ccugccuaga     1800

gagaagcggu ggcgcuuuga acccgaugcc auggcgagau ucgacacaga ggaaaaguuu     1860

cucgcucggc agcuggucga uacccaauac cuuucuagac uggcccgcga guaucucggg     1920

gugcuguucc cugacaaagg agaaggcagc gggcgggucu gggugucccc cggcagacug     1980

acagagaugc uucgccggag cuggggacuc aaccaccuuc ugccggauca uaaucucggc     2040

gggggagccg accagccuaa gaacagacgc gaccaccggc accaugcuau ugaugccauc     2100

guuguggcug ucaccgacag acaucugcuu caggccauug cgcgcgaggc cggccagaga     2160

gggcacgaag cugccaaucg gaugaccgcu gaucugcccg ugccuuggcc cgacuuugau     2220

cgcgacguug gacaagugau cagacagguc guggugucuc accgggccga ucacggcaca     2280

guuuccaaag ccggggcugc gaagggccag gacagcacgg ccggacgccu ccauaacgau     2340

accgcuuacg gccugacagg gcaaaccgac gagaaaggaa auacaauugu cgugagacgg     2400

gugccucuga ccgcccuuaa gaagcccacg ucuaucgaag ccgucagaga ugacgagcuc     2460

cgcaaagcuc ugagcgugug gacacggggc uaugauggga aggccuucga ggcgagacug     2520

ucccgcuuuc cggaacucgg accucugcag uuccggggca ucagacgggu ucgcgugguc     2580

gagccccuua ccgugauucc caucagagac ucuagcgggc ggguuuacaa aggcuauaag     2640

ggagacucca acuaccgcua cgaugugugg gaacugaaag acggcaagug ggucgcugag     2700

gugguuagca uguucgaugc ccaccagucu uccuggacca gcccugugag acgggaaaau     2760

ccgacagccc gcaagguccu cucucugcag caaaacgaca uuguggcuau cgagcccgag     2820

gggaaagaaa gagagcugau gcggguggcc cagcuucgcc agggaggcca gauuacccug     2880

gcggcuugcc augaagccgg ggaucucaag agacgggacg ccgaguccaa ugaagucgau     2940

ccuuuuaaau auuucagccc cacagcugga ggccugaaga gaguucgcgc ccggcaagug     3000

agaguggacg agcucggccg cguccuggau ccuggguccc gggacagaga gcgcggagcu     3060

gccuga                                                                3066


<210>  3756
<211>  3066
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3756
augaaguaca cccugggccu cgacgugggg aucgccagcg ucggaugggc uguuauugau       60

aaagacaaca auaagaucau ugaucugggc gugagaugcu ucgacaaagc cgaggaaucc      120

aagacagggg agucucuugc gacggcucgg cgcaucgcca gaggaaugcg gcgcagaauu      180

agccggcgcu cccagagacu gcggcucgug aaaaagcugu uuguccaaua ugaaaucauc      240

aaggauucua gcgaguucaa ccgcauuuuu gacaccucca gagauggcug gaaagacccc      300

ugggaacuuc gguacaaugc ccugucucgc auccucaagc cuuaugagcu ggugcagguu      360

cugacacaca uuaccaaaag acggggguuc aagagcaacc gcaaagagga ucucuccaca      420

accaaggaag gaguggucau cacaagcauu aagaauaacu cugagaugcu gagaaccaaa      480

aauuaccgga cgaucggcga aaugauuuuu auggagaccc cggagaacuc caauaagaga      540

aacaaagugg acgaauauau ccauacaauc gcucgcgagg aucuucugaa ugaaauuaag      600

uacaucuuca gcauucagcg gaaacucggc ucucccuuug uuaccgagaa gcuggaacac      660

gacuuccuua acaucuggga guuucagaga ccuuucgccu ccggggauag cauucugucu      720

aaagugggaa aguguacacu ccugaaggag gaacugcgcg cucccaccuc cugcuauacg      780

agcgaguacu uuggccuccu gcaauccauc aauaaccuug uccuggugga agacaauaac      840

acacucaccc ugaacaauga ccagcgggcc aaaaucauug aguacgcgca uuucaagaac      900

gaaaucaaau auucugagau uagaaagcug cuggauaucg agccugaaau ucucuuuaaa      960

gcccacaauc ugacacauaa gaaccccagc gggaauaacg aguccaagaa auucuacgaa     1020

augaaaagcu aucacaagcu gaagucuacc cucccuaccg acaucugggg aaaacugcac     1080

uccaauaagg agagccuuga uaaccuguuc uacugucuca caguguauaa gaaugacaac     1140

gaaaucaaag auuaccugca ggcuaauaac cuugacuauc ugauugagua caucgccaag     1200

cucccgacgu uuaauaaauu caagcaucug ucucuggucg cuaugaaacg cauuaucccc     1260

uuuauggaga agggcuacaa guauagcgau gccugcaaca uggcggaacu cgacuucacc     1320

ggguccucua aacuggagaa guguaauaaa cuuacaguug aaccuauuau cgagaacgug     1380

accaaccccg uggucauucg ggcccugaca caggcuagaa aggugaucaa ugccaucauu     1440

caaaaauacg gacucccuua uaugguuaac aucgagcugg cucgcgaagc cggcaugacc     1500

cggcaggaua gagacaaucu gaagaaagag cacgaaaaca aucgcaaggc ccgggagaag     1560

auuagcgauc uuaucagaca gaacggccgg guggcuuccg ggcucgacau ucugaaaugg     1620

cgccuguggg aagaccaggg aggcagaugc gcguacagcg ggaagcccau cccggucugu     1680

gaucuccuga augacucucu uacgcaaauc gaucauauuu auccuuacuc ccggagcaug     1740

gacgauucuu auaugaacaa agugcugguu cucacagacg agaaucagaa caagagaucc     1800

uacacccccu acgaggugug gggaagcacc gaaaaauggg aggauuuuga agcccgcauc     1860

uauuccaugc accuuccuca gucuaaggag aagcggcugc uuaauagaaa cuucauuaca     1920

aaagaccucg auagcuuuau cucccgcaau cugaacgaca ccagauacau uucucgguuc     1980

cugaagaauu auaucgaaag cuaccuccaa uuuuccaacg auagccccaa aucuugcguc     2040

guguguguca acggccagug cacagcucag cugcgcucca gaugggggcu uaauaagaac     2100

cgggaggaga gcgaccugca ccaugcccuc gaugccgcug ugaucgccug ugcggaccgc     2160

aaaauuauca aggaaauuac gaauuauuac aacgagagag aaaaucacaa cuauaagguu     2220

aaauaccccc ugccuuggca uucuuuccgg caggaccuga uggagacccu ugcuggagug     2280

uuuaucuccc gcgccccgag acggaagauu acaggccccg cccacgauga aaccauccgc     2340

agcccuaaac auuucaauaa gggccucaca ucugucaaaa ucccccugac cacggugacc     2400

cuggagaagc ucgagacaau gguuaagaac accaaagggg gaauuagcga caaggcugug     2460

uacaaugucc ugaaaaacag acuuaucgaa cacaauaaca agccucugaa agccuuugcu     2520

gagaagauuu auaaaccccu caagaauggc acaaacgggg ccaucauucg guccaucaga     2580

guggaaaccc cgagcuacac gggaguguuc cgcaaugagg gcaaggggau uucugauaac     2640

ucccugaugg uucgggucga cguguucaaa aagaaagaua aguauuaccu ggugccuauc     2700

uaugucgcgc acaugaucaa aaaggagcuu cccagcaagg ccauugugcc ucucaaaccc     2760

gaaucccaau gggagcugau cgacucuaca caugaauuuc uguucagccu cuaccagaac     2820

gauuaucugg uuauuaagac caaaaagggc aucacagagg gauacuacag auccugccac     2880

cgcggcaccg ggucucuuag ccugaugccu cauuuugcua auaacaaaaa ugugaagauu     2940

gacaucggag uccggaccgc caucuccauu gaaaaguaua acguggauau ccucggcaau     3000

aaaucuauug uuaaggggga gcccagacgc ggaauggaga aauacaacag cuucaagucc     3060

aauuga                                                                3066


<210>  3757
<211>  3072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3757
augaagaucc ugggcuucga cauuggggug gccagcaucg gaugggcuuu ugucgagggc       60

gaugaaauua gagacugcgg gguucggauc uucaccaaag ccgagaaccc caagacagga      120

gaaucccucg cgcugccucg cagagaggcu cggggcgugc gcagacggcu ugcccgcaga      180

cgggugcgcc ugaauacgau uaaaaagauc aucuguaaag aauuugaucu cagacugucu      240

gacuacauug ccgaugacgg ggagcuuccg aaggcuuauc agaccaacaa ggauacaaaa      300

agccccuacg agcugcggua uaaggcccuc aaugaacgcc uguccaaaga ggaacuggcu      360

agagucaucc uccacauugc caagcaucgg ggauacggca acaaacacgc gaaggacggc      420

gaugacaagg auucugggaa agugaagaaa gccaucggag agaauaagac ccugcuugag      480

caaaaaggcu augaaagcgu uggggaguac ugcuuccgcg aauuuuucaa guccauuaaa      540

gacaaggaug gagagacaca uaacaucccu ucucagaaua uuagaaacaa gaaaggcaau      600

uaugaccggu gugugagcca gaagcagcug gaagacgagc ucaaacugau cuuugcuaag      660

caaagaaaac uuggguucaa caaggaguuu gaagaugcca uccuggaggu cgcuuucuac      720

cagcgccccc ucaaggacuu ugaugacaaa auuggagauu gcaucuucuu ugacaaugaa      780

aagcgggccc cuaaagaguc cauuagcgcg aucgaguucg uggcccugac cagaauuauc      840

aacacacuga aguaccucuc uaaaaccacg ggcgaaguuu augagaagga aaucauuaau      900

aagaucuggc agaaaauucu ggagaacggc aagaugaccu acgcugaacu ucgcaaaauc      960

cuggcccucg auuccagcgu guucuuuaag gacaaagauc uggacuauac acuugggauu     1020

aaggaggcug aaaagaucaa auucaucgag uuuaagaaac ugauuaaguu caaaaaggcc     1080

cucggaucuu uugauggccu gucccgggag auccuggaca auauugccau gcagaucgcu     1140

auuaccaaag acagacaacg ccuccuggaa aaacuugagg ucuucccccu gaacaaggaa     1200

cagaaagaga accucagcca gcugaauuuu ucuaaguaca ucgaucuuuc ccugaaagcg     1260

cucaaccaga uucugccuuu caugcgggag gggagacgcu augacgaagc caaggaugac     1320

gcuaaacugc aagagaaggc caagaauaca aagaaggaag aguuucuccc gccccugaaa     1380

gaguucgauc uuaaccugac caauccugug guggcccggg cucucgccga auuuagaaag     1440

guccugaacg cgcugcuuaa aaaguacgga aagguucaca aaauccaucu cgaguucgcu     1500

cgcgacaugc ggcuggauuu ugacgaaaga gagcggcuga ucagcauuca gaaugauaac     1560

uucaauaagc gccagcucgc cgaagacuuc uguaaagagc acggccugga acccuccaac     1620

gccaauaucc uuaagauuaa acuguggcaa gagcagcagu ggcacugcau guauucuggg     1680

gagcucaucg gcauuccuga ucugaaggac cccacgaagc ugcaagugga ucauaucuac     1740

ccguacagca gauccuuuga cgacagcuau acaaacaaag ugcuuguccg ggcugaaugg     1800

aaucaggaga agggaaacca caccccuuuc gaagccuuug gcggggauaa aaauaagugg     1860

gacaaaaucg uggcucucgc cgagaagcug cccccuucua aaaagcugaa cagauccgaa     1920

aagagcaaaa aggauaaacu ccuggacgag aaguucaacg auaaagagcu uggauuucug     1980

gcgcgcaaua uuguugacac aggcuacauc gcccggcucg uggcuaagua uauugauaug     2040

uaccuggagu uccugccccu ugaagaaaac gagaauaccu cucucgccag aggggagaag     2100

ggaagcaaaa agcauaucgg cauugucaac gggcgccuga ccuccgaagu gagacacaau     2160

uggggccugg accccaaaga ucgggacaac caccuccauc acgcuaucga ugccaucguu     2220

auugcguaua ugaaugacaa gcgcaucaaa gccuuugcug auuucagacg gcugaaggag     2280

cuuaagauua aacgcuacgg aagagaaauc acagaggacg aauaucagga gaagaaaaag     2340

gagcugucuu gugccccuuu ugacgauuuc cggaaccgca cgcucgaaaa uauugacaaa     2400

aucuuuguga gcagaccgcc ccggaagcgc gcuagaggca cccugcauga ggaaacauuc     2460

uacuccaucg augacaagaa acugcuuaag gccuacgggg gaaaagaggg caugcagcgg     2520

gcccucagcc uggggaagau uagacagauc ggaaccaaaa uugucaagaa cggcacaaug     2580

gugcgcgucg auaucuuuaa ggacaaacgg gggagauucu auggcgugcc uauuuacacc     2640

auggauuucg cucugggagu ucuccccaau aaggcggccg ugucuggcaa agacaagaac     2700

gggcugauca aagaaugggc ugagauggau uccggauaug aguuuugcuu cagccuuuuu     2760

aaggacgauc ugauucucgu ccaacgcaaa gaaaugcagg agccugaacu guguuacuuc     2820

gugucuuuug acuccagcaa ugcccagauc gacguucggu cccacaacaa ucauuucucu     2880

gugcugaacg agaaccagaa acuucuguuu aagagcgcca cgcaagcuga agucaucggc     2940

gaguccauug ggauccagaa ucucaaggug uucgagaagu ggcacguguc uccccuggga     3000

gaaguuagaa aagccgaguu uagcccgcgc ggcgaagcga agcucaaaac auccaagaag     3060

cggaaaaagu ga                                                         3072


<210>  3758
<211>  3072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3758
augaagaucu acagcuucga cauuggcgug gccuccaucg ggugggcuuu uauugagaac       60

gaaguccugc aggauugcgg aaucagaauu uucaccaaag ccgagaaucc caagaacggc      120

gcgucucucg cucugccucg gcgcgaagcc agagggguuc ggaaacgccu ugccagacgg      180

cgcggaagac ugaauacaau caagcaacuc cuguguaaag aguuuggccu ucagcuggaa      240

gacuaucuca gcaacgaugg ggagcugccg aaggcuuaca cggccuccaa ggcuaauccc      300

cugaaaucuc cuuaugagcu ccggacccug gcccuuagcc agaagcugga cuccaaagau      360

cucgugcgcg ugauccugca cauugcgaag cauagaggau acggcaacaa acacgccaag      420

gacucuaagg auacagaaag cggcaaaguc aagaaagcua ucgaguucaa ucggaagacc      480

cuugccgaaa aaggguaucg cuccguggga gaguaccugu auagagaguu uuuccagcaa      540

agccggauuu cucagaacaa uggcgcuaca gaauuuauca acguucgcaa uaaggccggg      600

aacuacgagc auugcguggc gcaggacaug cucaaagaag aacuggaauc cauucugaau      660

gcccagagac aauucggauu uagccucaag caggauuucg agucuaagcu gcuuaccaaa      720

aucuuugagc agcggccccu gaaguccuuc gcugacaaag ucggcaacug uugcuuuauu      780

gccggggaaa agagagcucc uaaagauagc cccucugcca ucgaguucau cgcccucucc      840

cgcacaauua auacccugau caaccuuagc aaggaauccg gagagauuua caaccgggcu      900

aucauucugc agaucgugga aucuacgcuc aagaaaggcg agaucagcua uaaggagcug      960

agagaaauua uccgccugga cgagcggcuc agauucaaag auccucgccu ggacuacucc     1020

cgggaaauua aggaggcgga aaaaguuaag uuucuugagu ucaagcaacu gaaaggcuuu     1080

aagaaagccc ucggggagag cuucaccaca cuggcuaucu cucagcuuga cgaaauugcc     1140

gagcacaucg cccugaucaa ggauagagug aaacucaaug aaaagcugga guccuaugcu     1200

cugaccaagg agcagcgcga agcccucagc aaccugucuu uuagcgcgca uauuaaucuu     1260

ucccugcaag cucucaaaca gauccugccg uucaugcggg gagacaacgg cgagcagugu     1320

cuggauuacg acgaaucugu cgagaaggcc gggcuuaaag cccucagcaa ggauacacug     1380

aaaggacaga ugcugccccc ucucaaugaa uuugagcccu aucuguccaa cccuguggug     1440

gcuagagccc uugcugagua ccgcaagguc cugaauguuc uccugaaaca auauggcagc     1500

ccccacaaga uucacaucga auacgcccgg gaggcgaagc ugucuuccac cgaaagacag     1560

aaauacgaga aggaacagaa agacaacuau gccgcuaauc aggccgcuca uaagaaaugc     1620

caagagcuug ggcucgagcc gagcucuacg aaccugcuga agcucaagcu guggcaggaa     1680

cagggaggcc uuugucugua cuccggcgcc aaaauuacaa ucagcuaucu ccaggauccu     1740

accgcgcugc aaguggacca cauuuacccc uauucccggu cuuucgauga cagcuacaug     1800

aauaagugcc uggugcuuac aaaagccaac caggagaagg ggaaucgcac cccuuuugag     1860

gcuuucggaa ccgauuccgc caaauggggc gucaucacaa cgcucgcuca gaagcugccc     1920

cauaagaaaa gacggagaau ccugaacacc gccuuuaaug acaaggaggc cggguucaaa     1980

cuccgcaacc ugaacgacac aucuuauauu gcucggcuug uggcggauua caccgaaaca     2040

uaccuggagu uucucccccu gguugagagc gaaaauacca cgcuguccaa gggacagaaa     2100

ggcagcaaga agcacaucgc cauugugaac gggguccuua caucuaccau gagauauuac     2160

cucggcuucg cuuccaaaga ccgcgauaau caucugcacc acgcccugga cgccgugauc     2220

auuggauuca ugaacgauag cguuaucaag gcuuuuucug acuucaaaaa gacccaagag     2280

acauccaaag ccgcguauua cgcucauacc cucagcaagc aggaauauaa aaagcagaga     2340

gccuuuaucc aacugccuuc uggcgagaau uuccggguga gcguccuuga gaagguggaa     2400

uccgucuuug ugagcaaacc gccccgcaag agaguucgcg gggcccugca cgaggcuaca     2460

uucuacucuc cuaacgaucc caaacuccug aagaauuaug gaggcacgaa agggguggaa     2520

cgggcccugg cgcuuggaaa gaugagaaag gucggcgcua aaauuguguc caacggggcc     2580

augguucggg uggacaucuu ucgccauaag agcuccggca aauucuacgg agucccugug     2640

uacaccaugg auuuugcccu gggcauucuc cccaauaagg cugugguugu cgggaaagac     2700

cagaacggag ugaucaagga uuggcuggag auggacucua gcuaugaauu uauuuucucc     2760

cucuacaagg augacgugau ccugguccag aaaaaggaga ugcagagacc ggagcuuugu     2820

uauuucgugu cuuuugacac aagcggcgcc uccaucaaag uugcuaagca cgauaauaac     2880

uucucuaccc ugacagaaaa ucaaaaacuc cuguucagca acgccaccaa ggaggaagug     2940

gucgggaagu ccauuggaau ccagaaucug aaaguguuug agaaguggca gguuagcgug     3000

cuuggcgaag ucaaacggac gcagucuuac ccucgcgaga acauuucccu ggcgagcaag     3060

gccaaaaagu ga                                                         3072


<210>  3759
<211>  3075
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3759
augaucagaa cccugggcau ugacaucggg auugccagca ucggaugggc ugugauugag       60

ggcgaauaca cagauaaggg gcucgagaac aaagaaaucg ucgccuccgg aguucgggug      120

uucacgaagg cggagaaucc caaaaacaag gaaucucugg cccuuccucg cacccuggcu      180

agaagcgccc ggcgcagaaa ugcucggaaa aagggccgca uccagcaagu gaagcacuau      240

cucuccaaag cccuggggcu ugaccuggag ugcuuugucc agggagagaa gcucgcuaca      300

cuguuccaga ccucuaaaga uuuucugagc ccgugggaac ucagagagcg ggcccuguac      360

cgcgugcuug acaaggaaga acuggcgaga guuauucucc auaucgccaa acggcgcggc      420

uaugaugaca uuacauacgg cguggaggau aacgacuccg ggaagaucaa gaaagcuauu      480

gccgaaaauu cuaagagaau caaagaggaa caguguaaga ccauuggaga gaugauguau      540

aaacuguacu uccaaaagag ccuuaacguc cggaauaaaa aggaauccua uaaccgcugc      600

gugggcagaa gcgagcugcg ggaggaacuc aagacaaucu uucagaucca gcaggagcug      660

aaaucucccu ggguuaauga ggagcugauu uacaagcucc uggggaaccc ugaugcucaa      720

uccaaacagg aaagagaggg acuuaucuuc uaccagcgcc cccugaaggg cuuuggggac      780

aaaauuggaa aguguagcca caucaagaaa ggcgaaaauu cuccuuaucg ggccugcaag      840

caugcgcccu ccgccgaaga guucguggcu cucaccaaaa gcauuaacuu ucugaagaau      900

cuuacgaaca gacacggccu guguuucucu caggaagaua ugugcgucua ccucgggaaa      960

auccugcaag aggcccagaa gaaugaaaag ggacugaccu auuccaaacu caagcugcuu     1020

cuggaccucc cuagcgauuu ugaguuccug ggccuugacu acuccgggaa aaacccggag     1080

aaggcugugu uucugucucu ccccagcaca uucaaacuga acaagaucac ccaggaccgc     1140

aagacacaag auaaaauugc caauauccug ggagccaaca aggacuggga agcuauucuc     1200

aaagagcugg aaucccuuca gcugagcaag gagcagaucc agaccauuaa agaugcgaag     1260

cucaauuucu cuaaacauau caaccugucc cuggaggccc uuuaucaccu ccugccucug     1320

augcgggaag gcaagagaua cgacgagggg guggaaaucc uccaagagcg cggaauuuuu     1380

agcaagcccc agccuaaaaa ucggcagcug cuucccccgc ugucugaacu cgcuaaggag     1440

gagagcuauu ucgauauccc uaaccccguc cugagacgcg cccuguccga auuucggaaa     1500

guugugaaug cccuucucga gaaguacggc ggcuuccacu acuuucauau ugaacugacg     1560

agagacgugu guaaagcuaa gucugcccgc augcagcugg agaagaucaa caaaaagaau     1620

aaaagcgaaa acgaugcggc uucccaacuc cuggaggucc uugggcugcc uaauacauau     1680

aacaaucggc ucaagugcaa acuguggaag cagcaggagg aauacugucu guauagcgga     1740

gagaagauua ccaucgacca ccuuaaagau cagagagccc uccaaauuga ccaugccuuc     1800

ccccugucuc ggucccugga ugacagccag ucuaacaagg ugcucugccu gacauccagc     1860

aaucaggaaa aauccaacaa gacccccuac gaguggcuug gcucugacga gaaaaagugg     1920

gauauguaug uugggcgcgu guacagcucc aacuuuucuc cuagcaagaa aagaaagcug     1980

acccagaaaa auuucaagga gcggaacgag gaagacuuuc ucgcuagaaa ucuggucgau     2040

acaggauaua ucggccgcgu gacgaaagag uacaucaagc acucccugag cuuccuuccg     2100

cuccccgacg ggaaaaagga acacauucgg aucauuucug gcuccaugac cagcacaaug     2160

agaucuuuuu ggggaguuca agagaagaac cgcgaucauc aucugcacca cgcccaggac     2220

gcuaucauua ucgccuguau cgagccuucc augauucaga aauacaccac auaucugaag     2280

gauaaagaaa cccauagacu caagagccac caaaaagcgc agauccugcg ggagggcgac     2340

cacaaacuuu cucugcgcug gcccaugagc aauuucaagg auaaaauuca ggaauccauc     2400

cagaacauua ucccuagcca ucacgugucu cauaagguca cgggggagcu ccaccaagaa     2460

acagugagaa ccaaagaguu uuacuaucag gccuucggag gcgaggaagg ggucaagaaa     2520

gcucugaagu ucggaaagau ccgggagauu aaucagggca ucguggacaa cggggccaug     2580

guucgcgugg auauuuuuaa auccaaggac aaaggcaagu ucuacgcugu ccccaucuau     2640

accuacgauu uugccauugg aaaacugccg aauaaggcga ucgugcaggg caagaaaaac     2700

gggaucauua aggacuggcu ugaaauggac gagaauuaug aguucugcuu uagccuguuc     2760

aaaaacgauu gcaucaagau ucaaacaaaa gaaaugcagg aggccguucu cgcuaucuac     2820

aaguccacca auucugccaa ggcuacaauu gaacuggagc accucagcaa auacgcccug     2880

aagaacgaag acgaggaaaa gauguuuacg gauaccgaca aggagaaaaa uaagacaaug     2940

accagagagu ccuguggaau ccagggccuu aaaguguucc agaaggucaa gcugucugug     3000

cucggggaag ugcuggagca uaaaccucgg aaccgccaaa acauugcccu gaagacaacc     3060

cccaaacacg uuuga                                                      3075


<210>  3760
<211>  3078
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3760
augggcgggu acaugcccca gcugaugaga uaucggcucg cccuggacau cggaagcacc       60

uccauuggcu gggcuaugau ccgccuuucu augcaaaacg agccuauugc caucauuaga      120

augggggugc ggaucuucag cgauggacgc aauccgaagg acggcuccuc ucuggcgguc      180

acaagacggg uugcucgcca gaugagacgg cgcagagauc ggcuccugaa acgcaagacg      240

agacuucuga acgcccucgu gcagaaaaag uuuuuccccg aagacgccga ugcucggaaa      300

gcccuggugg uccugaaucc uuacgagcuc cgcaaggaag ggcuguauag acagcuuagc      360

ggacccgagu uuggccgggc ucuguuccac aucucccaaa gacgcggcuu ucucucuaac      420

cggaagaccg acaaaaagga uaaugacagc uccgugcuga aaaaggccau uguugaucuu      480

agacagcagc uggaagugga gaacugccag acaaucgggg aguggcuguu caaacaacag      540

cagagcggaa agucuguccg cgcgcggcuc agaggcaaga cccagaaaga caaggccuac      600

gauuuuuaua uugaccgcgc uaugaucgcc caugaguucg auacacugug ggugucccaa      660

gcuaaauacg acccucuccu guuuaaugaa gacgcccggg cgauucuuag agauauccug      720

cucuaucagc gcccccugaa gccuguuaaa ccggggcggu guacccuuau ucccgacgaa      780

agcagagccc cucuggcucu cccccugaca cagcaagccc gcaucuacca ggagcugaac      840

caccuccgga ucaccacgcc ugcucuggga gcccaggccc uuaccguggc ucagagaaau      900

acaauugcgg cccugcucga gaagucugau gucaccuucg ugaaaaucgc uaaggcccug      960

ggccuuacau ccaccacgga auuuaaccug caagacauua agcgggaucg ccucaaaggg     1020

aaugccacag cuaucauucu ggccagaaac caguacuucg gacggagaug gcaugacuuu     1080

aaugcggauc ugcagaaccu caucguggac aagcugguca augagggcag cgaaaccgcu     1140

cuuaucgagu ggcugaaagc caacacagau guugacgaau cugccgcuga gcgcgugucc     1200

ggcgugaccc uccccgaggg guauggaagc cuguccgucg ccgcucugcg gagacuucuc     1260

ccggcccuga aggaagaugu gguuguguac ucugaggcgg ucaaacgcgc cggcuucgac     1320

agccacuccg cucugagccu cauugaacag accggggagc uguaucaaga acuuccuuac     1380

uauggagagg ugcugcagag acauguuggc uucgggucug gagaccccaa cgauuccccu     1440

gagcggcgcu uuggcaagau cgccaauccc acagugcaca uuggccucaa cgaacugaga     1500

aagaucauua augcucugau caaacgguac gggaaccccg ccgaggucgu ggucgaacuu     1560

gcgcgcgacc ucaagcugag cagagagaaa cggaaggaaa uccaggagga gcagaaacaa     1620

cgccaggaua gaaaugaaaa gcggauuaag gacgccugcg agauccuggg aauugauccu     1680

cucgugaucg accgcggcaa aagacgggaa auuucucaga agaugcagcu gugggaugag     1740

cuuaacccga aagacgcugc caauagacgc ugucccuaua cgggggauca aaucagcauu     1800

gagaagcugc ucuccgcuga aguugagauc gaacacaucc ugccuuucuc uaaaacccug     1860

gacgauagcc uuaacaauaa gacaguggcc augcggagag ccaaccgcga caagggaaau     1920

cggacccccu acgaggcuuu uggcgaaucc cucaacccug auuacgacua ugacgcgauu     1980

cugcagagag ccagccugau gccccgcgag aaacggaaga gauucgcuuc ugauggguac     2040

cagcaauggc uccgcgacga uaaagacuuu cugucccggg cccuuaauga uacagccuau     2100

cugagcagaa ucgcuaagga guaccucacc cugauuugcc cgccuaacuc uguccgggcc     2160

auccccggcc gcaugacggc gcagcugaga gcuauguucg gacuuaaucg gcuccugucc     2220

gaaggcggga uuaaaaaccg cgaggaccau agacaccaug ccaucgaugc cgcugugauc     2280

ggaguuacag accagggccu gcuccagaag uuuagccggg ccuccgcgag aacccgcgaa     2340

cugcaacuuu cucggcuggu ggaugacaug ccucuccccu gggauaccua uagagagcac     2400

gucacacgcg cucuggaaca ccugguggug agccauaaac cugaccacgg guaccaggga     2460

aagaugcaug aggauaccgc cuggggccuu cgggacaacg ggcagguuac aagacgcguc     2520

cgguccgagg acggcggacc cagacagcgc cugcucgaaa auaagaacgu ggugaaaauu     2580

aauucuacga gagauuucag ccggcacggc aucgacgagc aagggcaggu caacgccuau     2640

aagggauacg ugggcggguc caauuacugu auugaaaucu ggauugauga gaaaggaaag     2700

uggaacagcg acguuguguc uaccuuucag gcuuaucaga ucguccgcga acugggcgag     2760

aaagccgcuu ggcaaaagcu cagaaauccg cugcuuuccc ugagcgccaa gccucucgug     2820

augcggcuga ucauugauga cguugugaaa augcagaucg cggggcagaa caagauuaug     2880

cgcgucguga aaaucgccca aaauggccag auuuucuuug cugagcauaa cgaagccaau     2940

guugaugcua gagacaagga uaaaaacaau cccuucaagu acaucucuaa gacagccgga     3000

ucccugcaga aagccggcgg gauccacaug gcuauuagcc ccaucggaga cgcgcggccu     3060

gugagauaug gccgcuga                                                   3078


<210>  3761
<211>  3084
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3761
augaaguaca cccugggccu cgacgugggg acaaacagca ucggaugggc cguccuggag       60

cuuaauacga acggcgaacc ccagagaauu gagaaacugg ggucccggau cuucaccgau      120

ggacgccacc cuaaggacgg cacaacccuc gcuucuaaaa gacggcaaaa gcgccaugaa      180

agacggcgca gagaucggcu gcaccagcgc aaaaagcugg uucuuaauca gcucgugagc      240

auggggcugu uuccgcaguc cgagcaagaa cagcaggacc ugaagucucu cgaugugcug      300

acacuuagag ccaacggagu cagcaaacag cuguccgcgu augagcucgg ccggguguuc      360

uacuaucuga aucuuaagcg cggcuuuaaa ucuaacagaa aagggggaag cgaggaagac      420

aaagcuaaua agauuuccga gcggaucaag aaacugcaag aacagcucaa ggagaacgau      480

uucaaaaccg uuggccagua ccugcaugac cgcuauauua aggggcugag cacaaaagcc      540

accaucgaga augauuuuca ccuccugaga cagcuuaucg aagacgaguu cgauuuuauu      600

guggaaacgc aaucuaaguu cuuucccgag aucuccgacg aacaguggaa cacccugcgg      660

aauagaaugu uccaucagcg cccucucaaa cccgucgaga caggaaccug cagccuguuu      720

gaggauaaga agcggacaua caaauauaug ccuucuuucg aagaguacag auuucuuacc      780

gaacuguuca accucuccua ccaagacgag aauuuccaca gccacucucu guccagcgaa      840

cagauuuuua agguguucca ugagcugaaa aacacgcgcg aggccacaua uaccaagauc      900

aaaaagcucc ugaaacuucc cuccgaccug ucuuuuagca uugaacgguc caagagcaag      960

gauaaaauca agauuucuac aaccaaucac uucuuuucca aaagccuccu guucgcugag     1020

gaaugggccu cucuugaccu gaagcucaag gaugagguug cugaaaucua cuuuagcgac     1080

auguccgugg aggagaucaa aaagggccug cugcagcuca aaauugauuc ugaaaucauu     1140

gagaaccugc uugaagagaa ggucccuaac gugcaggagg ccaccuguag cuauuccaaa     1200

gaagcgcugc aaaagaucgu gagccucauu cuggaggaag agagacaucc gacacugauc     1260

gucgacgauc uuuucaagaa ugaaaaagag gagacgucuc ucaucgaccg ccuggauuac     1320

uauggggccu ccauucccga cagcaugcag ccuauccccc agcacauuaa ggauaacaau     1380

cugacccuca acgaagacga gaaacgguac ggaaagaucg cuaauccuac aguucacgcc     1440

gcucugaacc agauuagaca aguggugaau gaccuuaucg aacaguaugg caaacccacc     1500

aacauccaua uugaguuugc ccgcgaucug aagaaaucua aggaggaacg ggacuucgau     1560

aauagaaaga aaaagaaaaa cgaggaacuc aaugagcagg uccgcgacuu uaucgaaaag     1620

cacaaccaga aaccguccgc guucaauuuu gagcggguga agcugugguu cgaacuggau     1680

gccaugaaca aucaaguuug cgcuuacagc ggcaagacaa uuuccgccag aauggugcuu     1740

ucugacgagg uccaggugga ucauauccuc ccuuucagcc ggacccugga cgauucccug     1800

gcuaacaaag uucucgugcu ggccucugag aacgucaaga aacgcaauag aacgcccuac     1860

gaagccuuug ggcaggacac agagaagugg gcuagcauuc aggaacuugc gaaacggcug     1920

ccuuaucaca agcaauggag auucgccgag gaugcuauca agaaauuuga ggaaaaggac     1980

caguuccucc agcgccaucu gaacgauacc cgguacauuu ccaaaaucgc caagaaauau     2040

cugggaagca ucaccgaccc cuacaagauu guggccucua gaggccaaau gacauccauc     2100

auucgcggga aacuuggacu caaucaguuu auccagaagc ccgacggcac caagaacaga     2160

gaggaucacc ggcaccaugc uauugacgcc cugacagucg cgcugacgag ccgcucuuau     2220

cucaaaagaa ucuccgaugc uagcgcccgg aaucaggacc cuaaccgcau cccgauuccc     2280

gaaccuugga agggguucau caaagaugcc gacgugaagu uuaaugagau uaucguuucu     2340

cacaaagugg aucauggaaa gaacggcccc uucauggaag agaccuguuu uggccugauu     2400

aagaauccua acaaauacga ggaagagaau gacuucaaac uuaucacaac caagagccug     2460

gauuccguca aggaccccag cacaaucaga ucucaauccc uccagaaccu ggcugaaaag     2520

agcgggguga uuucccuggc caagucugga guuaagaaac uucgggugua ugaugucacc     2580

aaggagaauc cggcugacau cggccucauu gaaagcgggc uggccaaaau cacgcacgga     2640

aaggauaaac agcacgugaa gcauuaccag aagggcgaca uuaacuaccu ggcgaucugg     2700

ugccucccuc aaaauaucac ccugaaaucc gacaaccaca agaaacgcaa gacagauuau     2760

auuuuuaccg ccaucaaaac auucgaccuu aacucuaaga aucccaacga ucugagaccu     2820

caucccgcug ccaagcucgu gaccaaaguu uacaaggggg acacggucgc ucuggaggug     2880

aauaaccagc ugaauuauua cauugugaaa agcauccggg ccgcgaacaa gaaacuuuau     2940

uuucugaaga uuaaucaguc cucugagcuc gccaacgaaa cacaguucau cccugcuuac     3000

accaagcugg ucgauuauaa auuccgcaag gugagcguuu cccccacagg cgugauuacc     3060

gacaauggac cgcuccugaa auga                                            3084


<210>  3762
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3762
augagaagcc ugcgguaccg ccucgcccug gaccuuggcu ccaccucucu ggggugggcu       60

cucuucagac uggaugccug caaccggccc acagcgguga ucaaggcugg aguccgcauu      120

uuuagcgacg gcagaaaucc uaaagauggg uccucucuug ccguuacgcg gcgcgccgcu      180

agagccaugc ggcgcagacg ggaccgccug cucaagagaa aaacccggau gcaggcuaag      240

cugguggagc acggauucuu uccggccgau gcgggcaaac gcaaggcccu ggaacaacuc      300

aaccccuaug cucugagagc caaggggcuu caggaggcuc ugcucccugg agaguucgcc      360

cgggcgcugu uucauaucaa ucagagacgc ggcuucaaaa gcaaccggaa gacagacaaa      420

aaggauaaug acuccggcgu gcuuaaaaag gccauugggc agcugagaca acagauggcu      480

gaacagggau cucgcaccgu cggcgaauac cucuggacac ggcugcagca agggcaggga      540

gugagagccc gcuaucggga gaagcccuac accacagagg aaggcaaaaa gagaaucgau      600

aaaagcuaug accuguacau ugaucgcgcu augaucgagc aggaauuuga cgcccucugg      660

gccgcucagg cggccuucaa cccuacccug uuucacgagg cugcccgggc cgaucuuaag      720

gacacgcugc uccaucaaag accccugcgc ccuguuaaac cggggcggug uacccuucug      780

cccgaggaag agagagcucc ucucgcccug cccuccacac agcgguuccg cauccaccag      840

gaagugaauc aucugagacu ccuggacgag aaccuucggg aagucgcgcu gacccucgcu      900

caaagagaug ccgugguuac agcccuggag accaaggcua aacuuagcuu ugagcagauu      960

cgcaagcugc ucaagcuguc uggauccgug caguucaauc uggaagacgc caaacggacg     1020

gagcucaagg gcaacgcuac aagcgccgcg cuggccagaa aagaacuuuu uggcgcugcc     1080

uggucugggu ucgaugaggc ucugcaggac gaaaucgucu ggcaacucgu gaccgaggag     1140

ggagaaggcg cccugauugc guggcugcag acacacaccg ggguggauga ggcccgcgcu     1200

caggccaucg ucgacguuuc ccuuccugaa ggauauggca aucucagcag aaaggcucug     1260

gcccggauug ugcccgcccu gcgcgcugcg gugaucaccu acgauaaagc cguccaggcu     1320

gccggguucg accaccauuc ucaacucgga uuugaguacg augccuccga aguggaggac     1380

cugguucacc cggagacagg cgaaauuaga agcguguuca agcagcuucc uuauuacggc     1440

aaggcucugc agcggcaugu cgccuuuggg uccggaaaac ccgaggaucc ugacgaaaag     1500

cgcuauggca aaaucgcgaa ccccacggug cacaucgggc ucaaucaggu uagaauggug     1560

gucaacgcuc ugauucggcg cuacggaaga cccaccgagg uggucaucga gcuggcccgg     1620

gaccuuaagc aaucucgcga acagaaagug gaggcccaga gacggcaggc ugauaaucaa     1680

agacgcaacg cccggauuag acgcagcauc gcggaaguuc ucggcauugg ggaggaacgg     1740

gugagaggcu ccgacaucca gaaguggauc ugcugggagg agcugagcuu cgaugcugcc     1800

gaccgccggu guccuuauuc uggaguccag auuuccgccg cuaugcugcu cagcgaugaa     1860

guggagguug aacacauccu gccguuuucu aagacacuug acgauagccu gaacaauaga     1920

accguggcca ugcgccaagc uaaccggauu aaaagaaauc ggacacccug ggacgcccgc     1980

gcggaguucg aagcccaggg cugguccuac gaggauaucc uccagagagc ugagcggaug     2040

ccucugcgca agagauaucg guuugccccc gacggguacg aaagauggcu gggagaugac     2100

aaagauuucc uugcucgcgc ccucaacgac acccgguacc ugucuagagu cgccgcugag     2160

uaucugcgcc ucgugugccc uggcacgcgg gugauucccg ggcagcugac agcgcuucug     2220

agaggaaagu uuggccucaa ugacguucug gggcuggaug gcgagaaaaa ccgcaaugac     2280

caucggcacc augccgucga ugcuugugug aucggaguga ccgaccaagg ccuuaugcag     2340

agauucgcca ccgccagcgc ucaggcccgc ggggauggac ucacaagacu ggucgacggc     2400

augccgaugc cuuggcccac cuaccgggau cacguggaac gcgcgguuag acauaucugg     2460

gugucccacc ggccugacca cggguuugaa ggagcuauga uggaggagac aagcuauggc     2520

auucgcaagg augggucuau caagcagaga cggaaagccg acggcuccgc cggaagagaa     2580

auuagcaacc ugauccgcau ucaugaggcu acgcaacccc uccggcacgg cgucucugcc     2640

gaugggcagc cucuggcgua caagggauau gugggcgggu ccaauuacug caucgaaauu     2700

accguuaacg acaaaggaaa gugggagggc gaggugauca gcacauucag agcuuauggg     2760

gucgugcgcg ccggcggaau gggccggcuu agaaaucccc augaagggca gaacggacgg     2820

aaacugauca ugcgccucgu uauuggcgau uccgugagac uggaggucga cggggccgaa     2880

cggaccaugc gcaucgugaa gauuucugga agcaauggcc agaucuuuau ggcuccgauu     2940

cacgaggcca acguggacgc uagaaauaca gauaaacaag acgccuucac cuacacgucc     3000

aaguacgcgg gcucucugca gaaggccaaa acccggcgcg ucacaaucag cccuaucggg     3060

gaaguuagag aucccggauu caagggcuga                                      3090


<210>  3763
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3763
augaaguaca gacuggcccu cgaccugggc agcaccuccc uugggugggc uaucgugcag       60

cugaacgcca aagaugagcc cugcgcgauu guccgggcug gaguucgcau cuucccugac      120

ggcagaaauc cgaaggaugg gucuagccuc gccgugacac ggcgcaacgc cagagcuaug      180

cggcgcagac gggaccgccu gcuuaaaaga aaggcccgga ugcaacgccu gcucauugaa      240

cacgaucugu uucccgcuga cgaggccgcg agaaaagccc uggaaacgcu caauccuuau      300

gagcugcggg cuaagggacu ugaugccgcu cugacccccg ccgaguucgc gcgcgcccuc      360

uuucaucuga accagagacg gggcuucaag uccaauagac gcacagaccg gaaagauaac      420

gacucugggg cucuuaaggc cgcuaucgga ggccugagag cccagcucga uaccgccggc      480

gcugacgggc gcgcgcggac agugggagaa cugcugugga gacgcaugag cgauccuaau      540

guccucaccg ugcgggccag auaccgcgag cagcggauua caaaagacga cggcaagacc      600

agaguugaua aauccuauga ccuguacgug gaucgcgcua uggucgaagc cgaguuugac      660

gcccuuuggg cuaagcaagc cgaacugucu cccacgcagu ucggggaggc ggcucgggcc      720

acccucaagg auugucugcu ucaccagaga aaccugcggc cugugcgccc gggaagaugc      780

acacucgagc ccgacgaacc ucgggcuccc cuggcucugc cuagccagca aagauuucgc      840

auccuccagg agguuaauca ucugcggcuu cugggcggga gacuccugga agagucccuu      900

agcguggaac agcgcgauaa acuggucgcc gcucucgagg acggaucuaa guccuucaaa      960

accgccaucc ccaagcugcu gggccucccg ggcacagugc aguuuaaccu ggcggaugcc     1020

aaaagaaccg agcuuaaggg gaaugcuacg agcaugcugc ucggcaaaaa ggacuucuuu     1080

ggaccugccu ggcacggguu cucugaagcu cggcaagaug agauugugau gcgcauccag     1140

gaagaggaau ccgaggccag acuggucgac uggcuggcga caagcaccgg aguugacgag     1200

gcccaugcuc uuaagauugc caacgcugcc cuccccgaag gcuauggguc ucuguccgcc     1260

aaagcucugg cgaagauccu cccugccaug cgggcugagg ugauuacaua cgauaaagcc     1320

gugcuggccg cuggauauga ccaccacagc cauaucuccc acgccgcguc uaccggcgaa     1380

auucuucccg agcugcccua cuacggcgaa uaucuccagc gccaugucgg guuuggaacc     1440

ggcgagcuga augauacacc ugagaagaga uucgggcgga ucgcuaaccc gacggugcac     1500

aucggacuga aucaaguucg cguggucgug aacgcccuua uuaaaagaua cggccacccc     1560

accgaaguua ucguggaggu cgcccgggac cucaagcaga gccaggauca gcgcagagaa     1620

acacaagagc agcaggcuaa gaaccagaaa cggaaugaga gacugcgcaa ggacgccgcg     1680

gcuauucugg ggaucgccga ugaacgggug agacucgccg acauucaaaa acugauccuu     1740

ugggaggaac uguccuucga uaucgcugac cgccgguguc cuuauagcgg cguccagcuc     1800

ucugccgcua uggugcuguc cgagcagguu gaaauugagc auauccugcc cuuuagcaug     1860

acccuugaug acucucucaa caauaagaca guggccauga gacuggucaa ccgccugaaa     1920

ggaaaucgga ccccuuggga ugcgagagag gccuucgcug gccaggggug ggacuacgac     1980

ggcauucuca ugcgcgccga acggaugccc agagcuaagc ggcaacgcuu ugccccggau     2040

ggacuggaga aguggcuuaa agaagacaag gauuuccugg ccagagcucu caacgacacg     2100

cggcaccuga gccgcguggc gagagaguau cugucccuug uuugcccugc cucuacacgg     2160

gugauccccg ggcagaugac cgcuaugcuc agagccaaau uuggacugaa ugauauucug     2220

ggccucaacg gggaaaagaa ucgcaacgac caucggcacc augccgucga ugcuugugug     2280

guggccguua ccgaccaggg ccugcuucag agauucgcgc aagcuagcgc cgaugcccgc     2340

ggaaaaggcc uggaccggcu cgucgagaca augccugauc ccugggacuc cuacagagag     2400

cacgugcagc gcgcugugaa uagcaucugg gucucucaca agccugauca uggguuugaa     2460

ggagccaugc acgaggacac cacauaucgg cccccgcauc uggacccuaa gaacaugugg     2520

agaacgcgcg gcaucggggg aggcaaaccc aaugaaaagg aggcggauuc caaagcuaug     2580

gugggguuca ccggccccga acaucugcag agacaccacc gggacaaggc cggacaacag     2640

cuuccuuaca aaggcuacgu ugccagcccg aaguuuugca uggauauugu gcgcaacgac     2700

aaagggagau gggagggaga ggucgugucu acauucgaag ccuaucggcu gguuuccgag     2760

uuuggccgcg augccgcucu cgcccacauc cagagcaagu ccagaucuau uagcgggaag     2820

ccccugguga ugcggcucca gggaggcgac auggucagau ucgaugacga ugaagggcgc     2880

ccugugguuc ugcggguggu caauaugacc ggcaacggac aaaucuuuuu cgcgccccau     2940

aacgaggcca auggcgacgc uagaaaccgc gauaaacagg accaguucgu guaccuuucc     3000

aaguaugccg ggucucugca gaaagcucgg ggaagacggg ugacagucag cccugccggc     3060

gagcuccgcg accccggguu uaaggaauga                                      3090


<210>  3764
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3764
augagaagcc ugcgguaccg ccucgcccug gaccuuggcu ccaccucucu ggggugggcu       60

cucuucagac uggaugccug caaccggccc acagcgguga ucaaggcugg aguccgcauu      120

uuuagcgacg gcagaaaucc uaaagauggg uccucucuug ccguuacgcg gcgcgccgcu      180

agagccaugc ggcgcagacg ggaccgccug cucaagagaa aaacccggau gcaggcuaag      240

cugguggagc acggauucuu uccggccgau gcgggcaaac gcaaggcccu ggaacaacuc      300

aaccccuaug cucugagagc caaggggcuu caggaggcuc ugcucccugg agaguucgcc      360

cgggcgcugu uucauaucaa ucagagacgc ggcuucaaaa gcaaccggaa gacagacaaa      420

aaggauaaug acuccggcgc ccuuaaaaag gcuauugggc agcugagaca acagauggcc      480

gaacagggau cucgcaccgu gggcgaauac cucuggacac ggcugcagca agggcaggga      540

gucagagcuc gcuaucggga gaagcccuac accacagagg aaggcaaaaa gagaaucgau      600

aaaagcuaug accuguacau ugaucgcgcc augaucgagc aggaauuuga cgcccucugg      660

gcugcgcagg ccgcuuucaa cccuacccug uuucacgagg ccgcccgggc ugaucuuaag      720

gacacgcugc uccaucaaag accccugcgc ccugugaaac cggggcggug uacccuucug      780

cccgaggaag agagagcccc ucucgcgcug cccuccacac agcgguuccg cauccaccag      840

gaaguuaauc aucugagacu ccuggacgag aaccuucggg aaguggcucu gacccucgcc      900

caaagagaug ccgucgugac agcucuggag accaaggcca aacuuagcuu ugagcagauu      960

cgcaagcugc ucaagcuguc uggauccguu caguucaauc uggaagacgc uaaacggacg     1020

gagcucaagg gcaacgccac aagcgcggcc cuggcuagaa aagaacuuuu uggcgccgcu     1080

uggucugggu ucgaugaggc ccugcaggac gaaaucgugu ggcaacucgu caccgaggag     1140

ggagaaggcg cgcugauugc cuggcugcag acacacaccg ggguggauga ggcucgcgcc     1200

caggcuaucg uggacgucuc ccuuccugaa ggauauggca aucucagcag aaaggcccug     1260

gcccggauug uucccgcucu gcgcgcggcc gugaucaccu acgauaaagc ugugcaggcc     1320

gccggguucg accaccauuc ucaacucgga uuugaguacg augcuuccga agucgaggac     1380

cuggugcacc cggagacagg cgaaauuaga agcguuuuca agcagcuucc uuauuacggc     1440

aaggcccugc agcggcaugu ggcguuuggg uccggaaaac ccgaggaucc ugacgaaaag     1500

cgcuauggca aaaucgcuaa ccccacgguc cacaucgggc ucaaucaggu gagaaugguu     1560

gugaacgccc ugauucggcg cuacggaaga cccaccgagg ucgugaucga gcuggcccgg     1620

gaccuuaagc aaucucgcga acagaaaguc gaggcucaga gacggcaggc cgauaaucaa     1680

agacgcaacg cgcggauuag acgcagcauc gcugaagugc ucggcauugg ggaggaacgg     1740

guuagaggcu ccgacaucca gaaguggauc ugcugggagg agcugagcuu cgaugccgcc     1800

gaccgccggu guccuuauuc uggagugcag auuuccgcug ccaugcugcu cagcgaugaa     1860

gucgaggugg aacacauccu gccguuuucu aagacacuug acgauagccu gaacaauaga     1920

accguugcua ugcgccaagc caaccggauu aaaagaaauc ggacacccug ggacgcgcgc     1980

gccgaguucg aagcucaggg cugguccuac gaggauaucc uccagagagc cgagcggaug     2040

ccucugcgca agagauaucg guuugcuccc gacggguacg aaagauggcu gggagaugac     2100

aaagauuucc uugcccgcgc ccucaacgac acccgguacc ugucuagagu ggcugcggag     2160

uaucugcgcc ucgucugccc uggcacgcgg gugauucccg ggcagcugac agcccuucug     2220

agaggaaagu uuggccucaa ugacgugcug gggcuggaug gcgagaaaaa ccgcaaugac     2280

caucggcacc augcuguuga ugccuguguc aucggaguga ccgaccaagg ccuuaugcag     2340

agauucgcca ccgcuagcgc ccaggcgcgc ggggauggac ucacaagacu gguggacggc     2400

augccgaugc cuuggcccac cuaccgggau cacgucgaac gcgcugugag acauaucugg     2460

guuucccacc ggccugacca cggguuugaa ggagccauga uggaggagac aagcuauggc     2520

auucgcaagg augggucuau caagcagaga cggaaagccg acggcuccgc uggaagagaa     2580

auuagcaacc ugauccgcau ucaugaggcc acgcaacccc uccggcacgg cgugucugcg     2640

gaugggcagc cucuggcuua caagggauau gucggcgggu ccaauuacug caucgaaauu     2700

accgugaacg acaaaggaaa gugggagggc gagguuauca gcacauucag agccuauggg     2760

aucgugcgcg ccggcggaau gggccggcuu agaaaucccc augaagggca gaacggacgg     2820

aaacugauua ugcgccucgu caucggcgau uccgugagac uggagguuga cggggcugaa     2880

cggaccaugc gcauugugaa gaucucugga agcaauggcc agauuuuuau ggccccgauc     2940

cacgaggcua acgucgacgc cagaaauaca gauaaacaag acgcguucac cuacacgucc     3000

aaguacgccg gcucucugca gaaggcuaaa acccggcgcg ugacaaucag cccuauuggg     3060

gaagugagag aucccggauu caagggcuga                                      3090


<210>  3765
<211>  3096
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3765
augcccgcca ucuacagacu ggcucucgac cugggcagca ccucccuugg gugggccgug       60

cugcggcucg agccugcggc ucuggaaccg auugccauca uuaaggccgg aguccgcauc      120

uucucugaug gcagaaaccc caaagacggg gcuagccuug ccguuacacg gcgcgcugcc      180

agagcgaugc ggcgcagacg ggaucgccug cucaagagaa aagcccggau gcaggacaag      240

cuggugaaac acggauuuuu cccuuccgau auggacgagc gcaaggcucu ggaaagacuc      300

gaucccuaug cccugcgggc uaagggccuu cgcgaggccc ugacgccugg ggaauuugcg      360

agagcccucu uccauauuaa ucaacggaga ggauuuaaau cuaaccgcaa gaccgacaaa      420

aaggauaaug acagcggcgc ucugaaaaag gccaucuccc accuucggca gcagauggag      480

gcuggcgggc auagaacagu gggagaucac cuguggucuc gccuccagca aggccugggg      540

guccgggcca gauaccgcga gacccccuuc acaaacgacg aaggaaagaa acggaucgau      600

aagagcuaug accuguacau ugacagagcc augaucgagc aggaauuuga ugcucucugg      660

gcggcccagg cugccuucaa uccugcccug uuucaugagg uggcucgcgc cgaccuuaaa      720

gauacccugc uccaccagcg gagacugcgc ccgguuaagc ccggccggug cacacuucug      780

ccugaggaag agagagcgcc ccucgcucug ccuuccaccc aacgguuccg cauuuaucag      840

gaagugaacc accugagacu ccugggggag gaccuucggg aagucgcccu gacgcucgcc      900

cagagagaug cuguggccaa ugcucuggag agcaaagccc gccuuaccuu ugagcagauc      960

cggaagcugc ucaaacuguc uggauccguu caauucaacc uggaagacgc gaagagaaca     1020

gagcucaagg gcaaugccac cagcgcugcc cuggcucgca aagaacuuuu uggcgccgcg     1080

uggggagggu ucggcgaggc ccugcaggau gaaauugugu ggcagcucgu cacagaggag     1140

ggggaaggag cucugaucgc cuggcugcaa gcucauaccg gcguggacga ggccagagcc     1200

caggcuauug cggaugccuc ucuucccgaa ggguacggau cccucagccg gaaggcucug     1260

gcccgcaucg ugccggcccu gagagcuggc gucguuacgu augacaaagc cgugcaggcg     1320

gcuggcuucg aucaccauuc ucagcucggg uuugaguacg acgccggaga aguggaggac     1380

uggguccacc cugauacagg cgagauccgg cccguguuca agcaacugcc uuacuauggg     1440

aaaccccuuc agcgccacgu ugccuuugga uccggcaagc ccgaagaccc ugaugagaga     1500

cgguacggga agauugcuaa cccgaccgug cauaucggcc ugaaucaggu ccgcacagug     1560

guuaacgccc ucauuagacg guauggacgc cccaccgaag uggucaucga gcuggcgaga     1620

gaccugaaac agagccggga gcaaaaggug gaagcucaga gaaaacaggc cgagaaucag     1680

cgccggaacg cccgcauuag aaaguccauc gcugaagucc uuggcaucgg ggaggaacaa     1740

gugcggggag ccgauauuca gaaauggauc uguugggagg agcucucuuu cgacguugcu     1800

gaaagacgcu gcccuuacag cggcgugcag auuuccgccc ggaugcugcu gagcgaugag     1860

gucgaagugg agcacauccu ccccuuuucu aagacccugg acgauucccu uaauaacaga     1920

acaguugcga ugcgccaggc caaccggauu aagagaaauc ggacgccuug gaccgcucgc     1980

gacgauuucg aagcccaagg guggagcuau gagggaaucc ugcagagagc ugagcggaug     2040

ccccuccgca aaagauaccg guuugccccg gacggcuaug aaagauggcu gggggaugac     2100

aaggacuucc uggcccgcgc ucuuaacgau acacgguacc ucucuagagu ggcggccgag     2160

uaccugcgcc uggucugucc uggcagcgga acccggguga uccccggcca gaugacagcu     2220

agacuccgcg ggaaauuugg acugaauggc gugcuugggc cugacggaga aaagaaccgg     2280

aaugaucaua gacaccaugc cguugacgcc ugcgucauug gcgugaccga ucaagggcug     2340

augcagcgcu ucgcuaacgc cuccgcgcag gcuagagagg acggccucac gcggcuggug     2400

gaugacaugc cccgcccuug gcccacauau agagcccacg ucgaacgggc cgugcgccac     2460

aucuggguuu cucauagacc ggaucacgga uuugagggcg cuaugaugga ggaaaccagc     2520

uacgggauug gaaaagacgg cuccaucaag cagcggagaa aggccgaugg gagcccugga     2580

cgcgagauua ccaaucugau ccggauucau gaagcgacac aaccccagag acacggcgcu     2640

gggcccgacg gccagccucu uccguauaaa ggauacgugg gcgggucuaa cuauuguauc     2700

gagaucaccc gcaaugauaa gggaaaaugg gagggcgaag ucauuuccac auuccgggcc     2760

uacgggaucg ugagaacgag cggacucggc cagcugcggc acccccgcgu uggccaaaac     2820

gggagagagc uggugaugcg gcucgucauu ggagacucug ugcgccugga aaucgacggc     2880

auugagagaa ccaugcggau cguuaagauc uccgggagca auggacagau uuuuauggcc     2940

ccuauccaug aagcuaacgu ggaugcccgc aauuccgaca aacaggaugc uuucgccuau     3000

acaucuaagu acgcgggcag ccuucagaaa accaaggcca gauucgucac aauuuccccc     3060

aucggggagg ugcgggaccu gggauuuaag ggcuga                               3096


<210>  3766
<211>  3099
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3766
augaagaaaa gaauccuggg ccucgacauu gggaucgcca gcgugggaug ggcucugguc       60

gaguucgaug acgaauuuca ggauaacaag aaaaagggca ccauuaucaa auccgggguu      120

cggauuuuca cacgcgccga gacgcccaag gaaagagagu cucuugcgaa gccucggcgc      180

gaagcuagaa gccaacggcg cagaauccgg cgcagagugg agcggcugaa uagcauccgc      240

gagcucuuug ugaaaaacgg agucguggaa gaguggucug uuagcaagaa aaauccgaac      300

aauauuuaca ucacccugaa ccagauuaag acacccuggc agcuuagaau ggaaucccug      360

gaccggaaac ucaccaauga gcaguucgcc gugguccuga cacaccuggc caagcaucgc      420

ggcuauaagu cucucagaaa agaugacaag gcugggaaag ugcuggaggc caucagccaa      480

aacaagaaac ggcuuaauga uuccgacuac agaacccugg gagaaauguu uugcaagaac      540

gagagcuaua aagaacacaa gcgcaauaag ggcggcgagu acauuaacuc uguugcucgg      600

gaacucaucg aggaggaagu ggagaaaauu uucgucaagc agagagaaaa agggaauaac      660

uuugccgaug ugaauacaga gcgcgaauau cugaagaucg cguucuccca gaaaccugcc      720

ggaagcaucu cuaagauggu gggcuuuugu accuucgaga acggggagaa gcgggcuccc      780

agagacuccu acagcgccga acuuuuuaaa guccugugca cgauuaacaa uaccacauuc      840

accgauauga agacaggaga gauccgcucu uuuuccaaag aagagauugg caagacccuc      900

gaaacgguua aaaacgugaa ggacgugaag uaugcuaaaa uccggaagga ucuggggcug      960

gaggacagcg ucaaauucuc cggaguggac uacagagaga augccaaguc uaaaggcgaa     1020

ggcgagauuu ucguuagcau gaagacauac caugaaauca aguccaaaau cgagaagcuc     1080

agcaaagaga agugggauaa ccugaaaggg aauaccgaac uucuggacuc uauugugaac     1140

aucauugcga aucucaagga ugacgagucc aucaaaaagg aauuucagaa gcugaaaauu     1200

gaugaggaca ucaucgaugu ccuucugaac uucgaauuua agaaauucau uaaucucagc     1260

uuuaaggccc ugcgcaaaau cauuccuuuc caauuucagg gacagaagua uaucacagcu     1320

ucuaccagcg ccggcuacuc ccugaagcag gaggggauua aaaacaagcu cgugcccccu     1380

gcuaaagaga auaucaacgu ugccgugguc gcccgggcuu ucgcgcaaac cagaaaggug     1440

auuaaugccg ucauccgcaa auauggacag uuugaccaga ucaacauuga acuggcuaca     1500

gagcuuaaaa auucuaaguu cgaucggagc aagaucgaag agggccaaaa gaaauuucag     1560

ucccagaagg acaaaauuuu caaggaagug caggagcuga gagggggaau gacgccgucc     1620

ucuucccucc ugcuuaagua ccggcugugg cgcgagcaag augaaagaug uguuuauagc     1680

ggccggaaaa ucucuuuuga ggaccuccug gacaccggcu uccuggauau ugaccacauc     1740

auccccuacu ccaagagcau ggaugacucc uucaacaaca aagugcucug ccuggccgaa     1800

gagaauagaa acaagaaaaa ugauauuccu uuuaacuauu accagcgcau cgaccgggau     1860

uggaaucuuc ugauuucuau cauuacaagc augaagaaca ugaaaaucgc caagaagucc     1920

agacuccuga aacaggaacu gucugacauc gagggguuca uugagcgcaa uaucaacgau     1980

accagauacg cuacacggua uauuaaggac uuucuugaaa auaaccucga guucaaagaa     2040

aaugaguuua ucaaggucaa agugcaggcc cgcagcggag gccugaccuc cguucugagc     2100

uacaacuggg ggaugucuaa ggauagagag gaauccuauu uccaucacgc gaaggacgcu     2160

auuaucgugg ccuguagcac gcaaggaaug guccaguaca ucucuaaaau uucccagcag     2220

uaugagaaug auaaggacaa aaugaagaaa aucaaggaca agacagaaaa cugcauuccc     2280

aaaccuuggg auagcuuucg ggccgacgug gaggaaucua ucgagggcau uuucgugagc     2340

uuugcucccc gccacaaggu uaccgggucc gcccauaaag agaccaucua cagcaagaaa     2400

caccucgaaa aggagagaua ugucacagug aagaaaucuc uggauaagau uaaacuuucc     2460

gaccuggaaa auaucccgug uaacgagaac ugcggcauca uucgggugcu caaggaacgc     2520

cuggagaaau ucaagaauga uagccugaag gcuuuugccg acccugucca uaugcccacc     2580

aaauguccua acaagaaagg acccgugauc agauccguua agauuaaaga gucuaauaag     2640

acaggcaucg aagugcggaa agggcuugcg gagcgcggag aaauggucag aguggauguu     2700

uucauuaagg acaagaaaua cuaccucgug cccaucuaug ucagcgauuu uaagaacaaa     2760

gaccugccua auaaggccau cgcugcccug aaacucgaga acgaauggau uaugaucgau     2820

aagacguacu ccuucaaguu cucucuguuu aaagacgauc uuauuaagau caaaaagaaa     2880

aaggaggaga uuuucggcua uuuuggggga gugcaccgga gcaccggcac aaucaagauc     2940

aaauccagac acaagccgaa agaaauugac gaggggaucg gcucucgcug ccugcucgac     3000

uuccggaagu uucaaguuga ugugcuggga aauuacaccg aagucaaaca ugagaagaga     3060

augcccgcuu auauuacacg caaggacaaa aagcacuga                            3099


<210>  3767
<211>  3105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3767
augaagaucc ugggcuucga cauugggauc gccagcauug gaugggcuuu uguggagaac       60

ggcgaacuca gagauugcgg gguccggauc uucaccaaag ccgagaaucc caagacagga      120

gacucccugg cgaugccucg cagagaagcu cggucuguuc gcagacggcu ugcccgcaga      180

aaaggccggc uggagacgcu caagcgccug cuugccaaag aaugggaucu guguuacgag      240

gacuauauug cugccgaugg ggagcucccg aaggcuuuua ugggaaagaa ccugaccaau      300

cccuacgugc ugagauauga agcccuccag cggcugcuug gcaaagagga acuggcgcgc      360

gugauccucc acaucgccaa gcauagaggc uacgggaaca aaaaugcuaa gauuacaaaa      420

agcgaggagu ccaagcggga acaaggaaag auccugucug cccuugcuac caacgccagc      480

gucauugcgc gcuauagaac agugggcgag uacuucuaua aagaauuuug cgagguuauc      540

aagaauccuc aggggcugaa caccaaugaa aacuguacac agcccaaagu gcggguccuc      600

aagccuauua gaaauaaagg aggcgaguac accaacugca uccugcagga ggaccugcaa      660

cgcgaacucc gguguauuuu cgagcaccag aagggguuug gauuccccau cacgcaggaa      720

uuucaggaua aaauccugaa gauugccuuc uaccaaagau cccuuaagga cuuuucucau      780

cuggugggca aaugcaccuu cuauccugau gagccgcgcg cucccaaguu uagccucucc      840

gccaucgagu ucauuacaaa agcuaagacc aucaaucugc uuguuggcau ugugaaagag      900

agcggggagg ucugggacaa ggaacagugg cgggagagac uggauucugu guucuccgcc      960

gugugugaac gcggaaaccu cacauuugcc cagaugcgga agauccugaa aaucgacgag     1020

cgcauucagu ucaaggaagu cgauuacacc gcuaaaaauc cugagaacaa gaaauuuguu     1080

gaguucaaga accugaagaa auuuugcgaa auccucggcg cgauuguggg ggacagagcc     1140

cuggcugaca gcaucgcccg ggauaugacg cuuauuaagg acgaggaaga acuggcccaa     1200

aaacucagag gauauggcaa guucucucag gagcagauca cacaacuggg ggcucuuaau     1260

uuuucccacc auaucaaccu gagccucaaa gcccugucug aaauucugcc cuucaugcgc     1320

gagggaaugc gguacgauga agcgugucag gaggcuaagc uccaggccaa gcacaaugac     1380

aaaaagucca aauuucugcc ucccuucugc ggcagcaucu augccgauga acuuaccaac     1440

ccgguggucc acagagcuau ugccgaguac cggaagaucc ugaaugcucu cauugccaaa     1500

uauggcaacg ugcauaagau ccacauugag cugacacgcg acguugggaa aaauuuucag     1560

gaaagagaga aguacaagaa agaaaucgag uccaacuaca aggcgcgggu gcaagccaug     1620

caggaaugug agaaacuggg acuuacccuc ucugagggca auauccugaa gcugagacuc     1680

uucauggaac agaacgagcg cugcgucuau agcgggcgga aaauuacccu ggcuaaucuu     1740

aaggaacagg gagcccugga gaucgaucau auucucccuu acuccagaag cucugacgau     1800

uccuauauga acaaggugcu gguuuuuaca aaugaaaacc aaaauaaagg caacaagacg     1860

cccuacgagg cuuucggggg cgacagccag aaauggggag agaucgaauc ucuggcccuu     1920

cgcagcggcu auccuaagaa aaaggcgaag cggauucucg auaaaggguu cggagacaga     1980

gaggccggcu uuaagucccg caacaucgug gacucugggu acaucgcuag acugauugcc     2040

aauuauacca aagaauaccu gaaguuccuc ccccuggaua gccacgagaa cacagcucuu     2100

aucgccggag agaaagacuc caagauucac gucgaagccg ugaagggcau gcugaccgcu     2160

acaaugcggc auuuuugggg gcucggcagc aaaaaucgcu acgagcacac ccaucacgcg     2220

aucgaugcca uuaucaucgc uuauauuaac gccgccauga uccagagauu cucucaguuu     2280

cggcaaaauc aggaaucccu gaaggcucgc uucuacgcca aagagcuggc gaaggaagag     2340

uuuaaaacgc agagagcuuu cuuugagccc uucagcggau uucgggaaca gguccuugcc     2400

aaggugggcc aaauuuucgu uucucgcccu ccgagaaaac gggccagagg ggcucuccau     2460

gaggaaacau uuuauuccau cgacgauaag aagcugagcg agaccuacgg aggcaaagaa     2520

ggggugcagc gcgcccuguc ccucggaaag auucggcaga ucggcaccaa aaucgucgcg     2580

aacgggccca uggugagagu ugacauuuuc aagcacaaaa agucuggccg cuucuaugcu     2640

gugccuaucu acacaaugga uuuugcccug ggcauucuuc ccaauaaggc cgucgugagc     2700

ggaaaagaca acgaucggau cauuaaggac uggcuggaga uggauuccaa uuaugaguuc     2760

ugcuuuucuc ucuucaaaga cgaugugauc gaaguucaaa agaaagacau ggagaagccu     2820

gaacuggcuu acuuugucag ccugaacacc uccagcgccu cuauugcucu ugugaagcac     2880

gacaauaaau ucgagacacu cacguccaac caggccaagc uguuuagcaa ugcgaaagaa     2940

aacaauguga ucgccgaguc ucuggggauc cagaaccuca aggucuucaa aaaguacaag     3000

gugagccccc ugggagagau uaaagaagcu aaguaugagc cgagacagcc uaucgcccuu     3060

aaaaccuccc ccaagaaaca ucgcaagcaa aacaaugcuc aguga                     3105


<210>  3768
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3768
augagacggc ugggccucga ccuggggacc aacagcaucg gauggugccu ucuggaucuc       60

ggcgacgaug gggagcccgu guccauuuuc cgcacaggag ccagaaucuu uucugacggc      120

cgggauccua agagccuggg gucccuuaaa gcuacgcgca gagaagcccg gcugacccgc      180

agacggcgcg acagauucau ucagcggcaa aagaaucuca ucaacgcgcu ggucaaauac      240

ggacugaugc cggcugauga gauucagcgc caggcccucg ccuauaagga ccccuacccu      300

auccggaaaa aggcucugga ugaagccauc gaccccuaug agaugggcag agcuauuuuu      360

cacaucaauc agcgcagagg cuucaagucu aaccggaaaa gcgccgauaa ugaagcgggg      420

guugugaagc aauccauugc cgaccuugag augaaacugg gagaggcugg cgccagaaca      480

aucggggaau uucucgcuga ucgccaggcc accaacgaca cagugcgggc gagacgccug      540

ucuggaacca augcccuuua cgaguucuau ccugaccggu acaugcugga acaggaguuu      600

gauacacucu gggcuaagca ggccgcuuuc aaccccagcc uguauauuga ggccgccaga      660

gaacgccuga aagagaucgu cuuuuuccaa cggaagcuca agccucagga agugggcaga      720

uguauuuuuc uguccgacga ggaucgcauc agcaaagcgc uuccgucuuu ccagcgguuu      780

agaaucuacc aggaacuguc caaucucgcu uggauugacc augauggggu ugcccaccgc      840

aucaccgcua gccuggcccu ucgggaccau cuguucgaug agcucgagca caagaaaaag      900

cugacguuca aagccaugag agcuauucug cggaagcaag gaguggucga cuaccccgug      960

ggcuuuaacc ucgaaucuga uaaucgcgac caucugaucg gcaaccuuac cuccugcauu     1020

augagagaug ccaaaaagau gaucgggagc gcgugggacc ggcuggacga ggaggaacag     1080

gauucuuuca uccucaugcu gcaggacgau caaaagggag acgaugaagu uagauccauu     1140

cuuacacagc aguauggccu gagcgacgau guggcugagg acugucucga uguccgccug     1200

ccugacgggc acggaucccu gucuaaaaag gccaucgauc ggauucuccc cgugcugaga     1260

gaccagggcc uuaucuacua ugaugccgug aaagaggcug ggcugggaga agccaaucuc     1320

uacgacccuu augcugcccu gagcgacaag cuggauuacu auggcaaagc gcuugccggc     1380

cacgucaugg gggcuuccgg aaaguuugag gacagcgaug aaaagcgcua cggcaccauu     1440

ucuaacccca caguucauau cgcccucaau caagugagag cuguggucaa cgagcugauc     1500

cggcugcacg ggaaaccgga cgaagugguu auugagaucg gacgcgaucu cccuaugggc     1560

gccgacggga agagagagcu ggaacgguuc cagaaagagg gccgcgcgaa gaacgaaaga     1620

gcccgggaug agcuuaaaaa gcugggacau auugacuccc gcgagagcag acagaaguuu     1680

cagcucuggg aacaacuggc uaaagagccc guggaucggu gcuguccuuu caccggcaag     1740

augaugucua ucagcgaccu guuuuccgau aaagucgaaa uugagcaccu ucuccccuuc     1800

ucucugacgc uggacgauag cauggccaau aagacagugu gcuuucgcca ggcuaacaga     1860

gacaaaggga aucgggcccc cuucgacgcc uuuggaaacu ccccugcugg cuacgauugg     1920

caggaaaucc ucgggagaag ccagaaucug ccguaugcga agcgcuggcg guuccuuccc     1980

gacgccauga agagauuuga ggcugaugga ggcuuccugg agcgccaacu caacgacacc     2040

cgguacauuu cuagauauac aaccgaauac aucuccacca ucauuccuaa aaauaagauc     2100

uggguuguga cagggcgccu gacgagccug cuucggggcu ucuggggacu caacucuauu     2160

cugagaggcc acaauaccga ugacgggaca cccgccaaaa agucccgcga ugaccaucgg     2220

caccaugcca ucgaugcuau ugucguggga augaccagca gaggccugcu ccagaaaguc     2280

uccaaggccg cgcggcgcuc ugaggaccug gaucuuacaa gacuguuuga agggcggauc     2340

gacccuuggg acggauuccg cgaugaggug aaaaagcaca ucgacgcuau uaucguuagc     2400

cauagacccc ggaagaaauc ccagggcgcc cuccacaacg auaccgccua ugggauugug     2460

gaacacgcug agaauggcgc cucuacgguc gugcauagag uuccgaucac aagccuggga     2520

aagcaguccg acauugagaa agugcgcgau ccucugauca agagcgcgcu ucucaacgaa     2580

accgcuggcc ugucugggaa auccuuugag aaugccgucc aaaaguggug ugccgacaac     2640

agcaucaagu cucugcggau uguggaaacc guguccauca uucccaucac agauaaagag     2700

ggaguugcuu acaagggcua uaaaggggac ggaaaugccu acauggauau uuaccaggac     2760

ccuaccagcu cuaaguggaa aggcgaaauc gucagcagau ucgaugcuaa ccagaagggg     2820

uuuauccccu ccuggcaaag ccaguucccu acagcccgcc ucauuaugcg gcugagaauc     2880

aacgaccuuc ugaagcucca ggauggcgag auugaggaaa ucuaucgcgu gcagcggcug     2940

ucuggaucca aaauucugau ggcgccccac acggaggcca auguggacgc uagagaccgc     3000

gauaagaacg acaccuuuaa acuuacaagc aaguccccgg gcaaacugca aucugccagc     3060

gcuagaaagg uccauaucuc cccuaccggg cucauucggg aaggauga                  3108


<210>  3769
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3769
auggagcagc ugaacuacau ccucggccug gacauuggga ucgccagcgu gggaugggcu       60

gucguugaaa uugaucacaa ugaggacccc aucagacuua uugauguggg cgugcggacc      120

uucaacaagg ccgaagucgc gaaaacaggg gagucccugu cucucauccg cagacuggcu      180

cggggaagcc gcagacuugu gcggcgcaag aaaaugagac ugcucacggc caagcggcug      240

cugaaauccg aaggcauccu cucugaccug gauuuuaaua agggggccau ucuuaaccug      300

ccuaauaacu gcugggagcu ccgcguuaag ggacuggaca aaaagcugag cgauaaagag      360

ugggcugccg ugcugcucca uaucauuaag cacagaggcu aucuguccca gcggaaacau      420

gaccgcaaga ccgaugacaa ggaacugggc gcucuccugu cuggggucag caaaaaucac      480

ucccuucugg aggaaggaga guucagaaca gccgcggagc ucgccaucaa cguguuuugu      540

aauagcaagg gccauauucg gaaccagugg ggagauuacu cucacaccuu cucccgccug      600

gaccuucaga aagaaaucaa gcagcuguuu guuagccaaa gagagcucgg aaauccguau      660

acaucuucca gcuucgaaga gaacauuauc ucucugcuga ugaaacagaa ggaagcuauc      720

uccggcaaug auauucucaa aaugcugggg aagugcaccu acgagaagaa cgaguauaaa      780

gccgcuaaga auacauacag cgccgaacgg uuuguguggc uuaccaaacu gaacaaccuc      840

agaaucgucg acgacggagu ggagcgcgcg cuuaauuccu cugaacggaa acuucugauu      900

gaugagcccu auaagaaggc caagcucacg uacgcucagg ugagaaaaau ccuggaacug      960

ccugacagcg ccgucuucaa gggcguucgc uacuuugcug auaacgccga guccagcacc     1020

cucauggaga ugaaauucug gcacucuauu cggaaggccc uggaauccaa uaaccuuaaa     1080

gacgaguggc uggugcucaa gacaaagccc gaacugcugg augagaucgg caccggguuu     1140

agcaucuaua aaacagacga ugacauuacc ucuuaccuua gcaauaagcu cgauaaaaag     1200

gugcuggaca uccugcucgu cgaagugucc uucgauaaau uuauugagcu gucucuuaag     1260

ugucugagaa agaucauucc ucucauggag aaaggauauc gcuacgacga ggcuuauaag     1320

uacaucuaug gcgagguuaa caucgaauac aaucugaacg acauuaaaaa ucccgugguc     1380

cugcggagcu ucucccagag cagaaaggug aucaacgcgg uuauucgccu uuacgggucu     1440

ccugcccgga uccauauuga gacggcuaga gauaucggaa aauccuucaa ggagcgcaaa     1500

gccauugaaa agcaacagga gaagaaucgg cucgaaaaag ugagagccgc ugagcacuuu     1560

aagcagcugu ucccgaacua ugucggcgaa cccaaagcca aggacauccu gaaagugcgg     1620

cucuacgagc agcaacaggg gaagugccug uauagcggaa agauccuuga uauuaaacgc     1680

cugauggagc auggcuacgu cgaaaucgac cacgcgcucc cuuuuucuag aacaugggau     1740

gacuccuuca auaacaaggu gcugguucug gcuaaugaga accagaacaa aggcaauaag     1800

acccccuaug aaugguuuaa cgggaaagau aauagcgacu ccuggaagcg guuccuugcc     1860

cucgugaaca auucuggauu uagcgccguc aagaaacaac acauucugau gaaggagauc     1920

gaugaagagg gcuucaaaca gagaaaccug aaugacacac gcuacauuac ccgguuucuc     1980

uguaacuggg uggagaauaa caucccucug cuugggaagg gaguuaaaag aguguucgcu     2040

ccccagggca agaucaccgc ccugcuccgc uccaauuggg ggauuagaaa gguccgggaa     2100

gauaacgacc gccaucacgc uaucgaugcc auuguggugg cgugcacaac gguuucuauc     2160

cagcaaagaa uuaccaauua ugucaaaaac agcgagauca aucggaagau caaacgccag     2220

aacuaccuga aguuuccgga accuuggaaa uucuuuagag aggacauuuc cauccgggug     2280

uucagcaaca aucccauuga agaacugaag cagcagcuuu cugaccgccc ugaggccaac     2340

caucaauucg ugacaccccu cuuugucucc agagcucccg ugcggaaaau gaccggccag     2400

ggacacgaag auacaauccg cagcgccaag agaauuucug agggcaucuc cguucugcgg     2460

gugccucuga ccaagcucaa ugacaaaagc cuggaacuua uggucaacag agagcgcgag     2520

aaggaacugu acgaugcucu caaacagcgg cugcuggagu ucaagaauga cccggaaaaa     2580

gccuuugcgg agcccuucua uaagaaaggg ggacagcaag ugaagacggu uagaaucgaa     2640

cgcgugcaga agaacggcgu ccuugugaau aacaauacag ggauugccga uaacggaaau     2700

aucguucggg uggacgucuu ucugaaaaag aaaaaguacu uccucgugcc uauuuauucu     2760

uggcaagugg cuaagggcau ccugcccaac aaagccauug ucgcucauaa gaaugagugu     2820

gauugggccg acauggaugg gagcgcccag uuucucuucu cccuguacac caacgaccuu     2880

aucgagguug ugcugaaaga uagcaccauu cucggcuauu uuucuuccau cgacagagcu     2940

acagcggcca ucauuaucaa ggaaccugau auggagaaaa gcaagggaaa ggaaggcauu     3000

caccgcgugg gggucaaaac cgcucggucc uucaucaagu acaaugugga cguucugggg     3060

aaaaacauuu cuagaugccg caaggccaaa cggcugcccg ugagauga                  3108


<210>  3770
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3770
augcugagaa agaugcacua ccggcucgcc cuggaccuug gcagcaccuc ccuggggugg       60

gcuaugaucc gccucaacgc cggaaaugag cccugcgcgg ugauuaaagc uggcgucaga      120

aucuucucua acgggcggaa uccuaaggau ggaagcuccc uggccguuac acgcagagaa      180

gcccgggcua ugcgcagacg gcgcgacaga cuucugaaac ggaaggcccg caugauucag      240

acgcucaucg aguauggcuu uuucccggcu aaagaagauc aaagaaaggc ccuggagauu      300

augaaccccu uugcgcugcg ggccaagggg cucaaugaag cucugacccc ugccgaguuc      360

gcucgcgccc uuuuucauau caaccagaga cggggauuca aaucuaauag aaagacagac      420

aaaaaggaua acgacagcgg cgcgcugaaa accgccauca auaagcuccg cgagacacug      480

aagaccgaaa acugucggac agugggcgag uggcuuaaua aaagagaugu ggcugggcug      540

accguccgcg cccgguacca ccaggacaag acgauuaaag aagauggaaa guccaaaauc      600

aacaaguauu acgaccucua uauugauaga gcuaugaucg agcaugaguu ugacgaacug      660

uggaaaaagc aggccgagcu gaaucccacc cucuucacag aagccgcucg cgcggaucug      720

aaggacgugc uucugcacca acgggcccuc aaaccuguua agcccggcag augcaccuuu      780

augccugacg aggaacgcgc uccgcuggcc cuucccucua cacagcgguu cagaauguac      840

caggagguga acaaucugcg cauucuccgg gaugggcuga aagaggaaag ccugacccuc      900

aaccagagag acgaucugau cgccgcucuu gaggaaaaca auaagcggac guuuacacaa      960

auuaaaaagc ugcucggacu gggcgggguc gugaaguuca acuuugagga cccuaaacgc     1020

gaggaacuua agggaaauac cacauccgcc auccugggca aagaugagca uuucggcagc     1080

guuugguuca aguuugacga agcgaaacag gaugcuaucg ugaugcagcu uuucaaggag     1140

gaaaacgagg ccaagcugcu gagauggcuc cagaaugaaa ccgggauuga cgagaaacgg     1200

gccgagacca ucgcuaacgu cggacugccc gaaggcuaug ggucucuuug ugcccuggcu     1260

cucgcccgca uucugccuga gcugagacgg gauguggugc ccuacuccga cgcggccaag     1320

gcugccggau uucaccacag cgauacaucu aauaaccaag aagucccggg ccuuacguuc     1380

ccuaucgagc ucguugugac cgagacaggg gaaauuaaaa ccaagcauau cuuucacgag     1440

cugcccuacu auggagaaua ccugcagaga caugugggcu ucggcuccgg gaaaccugag     1500

gacagcgcug aaaagcgcua uggaaagauc gccaauccca caguccacau uggccucaac     1560

caggugagac ugguugugaa uacccuuauc aaacgguacg ggcaccccuc ugaggucauu     1620

guggagguug cgcgcgaccu gaagcaaucc aaagaucuca gagacgaaga gggaaagcgg     1680

caggccgaaa accagaaacg caauaagaga auccggacgg cuauugccgg cgugcugcag     1740

acaagcgagg aacgcgucag acgggaugac aucgagaaaa ugauccugug ggaggaacuu     1800

uccuuugauc cugcugaccg cagaugcccg uauucugggg ugcaaauuag cgccgccaug     1860

cuccuguccg augaggucga aaucgagcau auucugcccu ucagccagac ccucgacgau     1920

ucucugaaca auaagaccgu ggcucuccgg caggcgaaca gaguuaaggg caaccgcaca     1980

ccuugggaag ccuuuggagc ucaguccacc gccggcuucg acuacgccac aauccuggcu     2040

cgggccgagc acaugcccaa agggaagaga uaucgcuuug cggaggaugg auaccaacgg     2100

uggcucaaag acgaugcugg cuuccugccu agagcccuga augacacgcg ccauaugagc     2160

aagguggccc gggaauacau gucucuuauu ugucccaaca ccagagucau cccggggcgc     2220

augacagcua ugcuccgggg aaaauuuggc cugaaugacg ugcuggggcu caacggcgag     2280

aagaauagaa acgaucaccg gcaucacgcc guugacgcgu gcgugauugc ugucaccgau     2340

cagggacugc uucagcgcuu cgccaaggcc agcgcuuccg ccagagaaca gcaacugaau     2400

cggcucgugg agaacaugcc ucugcccugg gagucuuauc gcgaacacgu ggagagagcu     2460

aucgacgcca ucuggguuag ccauaaaccu gaucacuccc augaaggcgc gaugcacaau     2520

gacacagccu acgggcugcu uggagauggc aaggucagca uucacaaaau gguggagggg     2580

aagcgggaaa gaaucgagga gucucucaaa gugauugcuu ucaccaacgc caaggcuaau     2640

gaccgccaug gacugcugga uaacggcgaa ccccggccuu auaaggggua caaaggcgac     2700

uccaauuauu guaucgagau uaucagaaac gaaaagggaa aaugggaggg cgaagucauc     2760

agcacguuug augccuacca gcucgugcgc gagcacgggg uugcccggcu gagacauccc     2820

acccuuucug uguccggaaa gccgcugguc augcgccuca ugaaagacga gacaauucgg     2880

auggugacca augaaaagcc uaucacagcu agaguuugcu gggugcgcag cgauaccaga     2940

auugcguucg ccuccacguc ugaggcuaac gucgacgccc gggaccgcga uaagaacgac     3000

agcuuuuccu auaucgugaa aacagccucu guucugcaga agcugcaggc uagacggauu     3060

agcaucuccc ccaccggcga acuucgcgau cccggguuca aagaguga                  3108


<210>  3771
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3771
auggagcagc ugaacuacau ccucggccug gacauuggga ucgccagcgu gggaugggcu       60

gucguugaaa uugaucacaa ugaggacccc aucagacuua uugauguggg cgugcggacc      120

uucaacaagg ccgaagucgc gaaaacaggg gagucccugu cucucauccg cagacuggcu      180

cggggaagcc gcagacuugu gcggcgcaag aaaaugagac ugcucacggc caagcggcug      240

cugaaauccg aaggcauccu cucugaccug gauuuuaaua agggggccau ucuuaaccug      300

ccuaauaacu gcugggagcu ccgcguuaag ggacuggaca aaaagcugag cgauaaagag      360

ugggcugccg ugcugcucca uaucauuaag cacagaggcu aucuguccca gcggaaacau      420

gaccgcaaga ccgaugacaa ggaacugggc gcucuccugu cuggggucag caaaaaucac      480

ucccuucugg aggaaggaga guucagaaca gccgcggagc ucgccaucaa cguguuuugu      540

aauagcaagg gccauauucg gaaccagugg ggagauuacu cucacaccuu cucccgccug      600

gaccuucaga aagaaaucaa gcagcuguuu guuagccaaa gagagcucgg aaauccguau      660

acaucuucca gcuucgaaga gaacauuauc ucucugcuga ugaaacagaa ggaagcuauc      720

uccggcaaug auauucucaa aaugcugggg aagugcaccu acgagaagaa cgaguauaaa      780

gccgcuaaga auacauacag cgccgaacgg uuuguguggc uuaccaaacu gaacaaccuc      840

agaaucgucg acgacggagu ggagcgcgcg cuuaauuccu cugaacggaa acuucugauu      900

gaugagcccu auaagaaggc caagcucacg uacgcucagg ugagaaaaau ccuggaacug      960

ccugacagcg ccgucuucaa gggcguucgc uacuuugcug auaacgccga guccagcacc     1020

cucauggaga ugaaauucug gcacucuauu cggaaggccc uggaauccaa uaaccuuaaa     1080

gacgaguggc uggugcucaa gacaaagccc gaacugcugg augagaucgg caccggguuu     1140

agcaucuaua aaacagacga ugacauuacc ucuuaccuua gcaauaagcu cgauaaaaag     1200

gugcuggaca uccugcucgu cgaagugucc uucgauaaau uuauugagcu gucucuuaag     1260

ugucugagaa agaucauucc ucucauggag aaaggauauc gcuacgacga ggcuuauaag     1320

uacaucuaug gcgagguuaa caucgaauac aaucugaacg acauuaaaaa ucccgugguc     1380

cugcggagcu ucucccagag cagaaaggug aucaacgcgg uuauucgccu uuacgggucu     1440

ccugcccgga uccauauuga gacggcuaga gauaucggaa aauccuucaa ggagcgcaaa     1500

gccauugaaa agcaacagga gaagaaucgg cucgaaaaag ugagagccgc ugagcacuuu     1560

aagcagcugu ucccgaacua ugucggcgaa cccaaagcca aggacauccu gaaagugcgg     1620

cucuacgagc agcaacaggg gaagugccug uauagcggaa agauccuuga uauuaaacgc     1680

cugauggagc auggcuacgu cgaaaucgac cacgcgcucc cuuuuucuag aacaugggau     1740

gacuccuuca auaacaaggu gcugguucug gcuaaugaga accagaacaa aggcaauaag     1800

acccccuaug aaugguuuaa cgggaaagau aauagcgacu ccuggaagcg guuccuugcc     1860

cucgugaaca auucuggauu uagcgccguc aagaaacaac acauucugau gaaggagauc     1920

gaugaagagg gcuucaaaca gagaaaccug aaugacacac gcuacauuac ccgguuucuc     1980

uguaacuggg uggagaauaa caucccucug cuugggaagg gaguuaaaag aguguucgcu     2040

ccccagggca agaucaccgc ccugcuccgc uccaauuggg ggauuagaaa gguccgggaa     2100

gauaacgacc gccaucacgc uaucgaugcc auuguggugg cgugcacaac gguuucuauc     2160

cagcaaagaa uuaccaauua ugucaaaaac agcgagauca aucggaagau caaacgccag     2220

aacuaccuga aguuuccgga accuuggaaa uucuuuagag aggacauuuc cauccgggug     2280

uucagcaaca aucccauuga agaacugaag cagcagcuuu cugaccgccc ugaggccaac     2340

caucaauucg ugacaccccu cuuugucucc agagcucccg ugcggaaaau gaccggccag     2400

ggacacgaag auacaauccg cagcgccaag agaauuucug agggcaucuc cguucugcgg     2460

gugccucuga ccaagcucaa ugacaaaagc cuggaacuua uggucaacag agagcgcgag     2520

aaggaacugu acgaugcucu caaacagcgg cugcuggagu ucaagaauga cccggaaaaa     2580

gccuuugcgg agcccuucua uaagaaaggg ggacagcaag ugaagacggu uagaaucgaa     2640

cgcgugcaga agaacggcgu ccuugugaau aacaauacag ggauugccga uaacggaaau     2700

aucguucggg uggacgucuu ucugaaaaag aaaaaguacu uccucgugcc uauuuauucu     2760

uggcaagugg cuaagggcau ccugcccaac aaagccauug ucgcucauaa gaaugagugu     2820

gauugggccg acauggaugg gagcgcccag uuucucuucu cccuguacac caacgaccuu     2880

aucgagguug ugcugaaaga uagcaccauu cucggcuauu uuucuuccau cgacagagcu     2940

acagcggccc ugaucauuaa ggaaccugau auggagaaaa gcaagggaaa ggaaggcauc     3000

caccgcgugg gggucaaaac cgcucggucc uucauuaagu acaaugugga cguucugggg     3060

aaaaacaucu cuagaugccg caaggccaaa cggcuucccg ugagauga                  3108


<210>  3772
<211>  3111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3772
augagaaucu ucggcuuuga cauugggauc accagcauug gaugggccua cguggagucc       60

aacgaacuga aggauugcgg cguccggauc uucacaaaag cugagaaucc caagaacggg      120

aaaucucucg cccugccucg cagagaagcg cggggagcuc gcagacggcu ugcccgcaga      180

aaggcccggc ugaaugcuau uaaacgccuc cuguguaagg aguuuggccu uaacguuagc      240

gacuauuucg ccaaugaugg ggaacugccg aaggcuuacg agacgaccaa agacacaaag      300

ucccccuaug agcucagauc ucuggcccug gaacagaaac ucgagccuaa ggaacuggcg      360

cgggugaucc uucacaucgc caaacaucgc ggauacggca acaagcacgc uaagaccggc      420

agcgauaaag acuccgggaa ggugcugucu gccauuaaag ucaaugagac agcuaugaag      480

gauaaauauc aaagcguggg agaguaccuc uauaaggaau uuuaccagaa acugagacuu      540

gaguccgaaa agaagggcaa caauaucacc aaagaguuca agaacguucg gaauaaaggg      600

gaaaacuacg agcauugcgu ggcccaggag cagguccgcg cggaacugga gcucauuuuu      660

gccaagcaaa aagacuucgg agcuacauuu agcgaaaauu ucgagucuca gaucauugaa      720

accgccuuuu aucagagaga ucugaagucc uucgaggaca aggugggcaa auguguguuu      780

uacgagaacg aaaaucgggc ucccaaggau agccugucug ccauggaguu cguugcccuc      840

acgagaauca uuaacacccu gaaaaaccuu gaagagaagu ccaaaaaucu ggggaucgga      900

gaagucuaug gcaaggacaa gauccaggag auucucaaaa ucgugcugga uaagggcgag      960

augagcuaca aaaagcugcg cgaaauucug caccucgacg agcauauccu guucgcuaaa     1020

gacucuaagc uggauuauac acaaggggug caggaagcgg agaaggccaa auuuauugag     1080

cucaagaacc ugaaagccuu caagaaagcu augggaggcg acuuuuccaa uuucgcccgg     1140

gaagaacuug auaagaucgc uaccgacauc acacugguca aaagcaagga aaaccucgcc     1200

aagaaacugc agaauuacuc cgcgcuuucu aaggagcagg uugaagcucu gagcaaccuc     1260

uccuuugcca aacacauuga ucugagccug aaggcccucg agcaaauccu gccuuucaug     1320

agaggggaca aaaccggaaa gugccuucgc uaugaugagg cugugaagaa agccgaacug     1380

caggagcacc ggaagcauaa acagaagggc gaauuucuca uuccccugaa agacuacgag     1440

ccuuaccugg cuaauccggu ggucgccaga gcgcuuucug aauaucgcaa ggugcucaac     1500

gcccugcugc ggaaguacgg gaaaguucac aagauccauc ucgaguucac gagagaggcu     1560

aaacuguccg ccaaggaacg ccaaaaauau gagaaggaac agaaggagca cuuugaagcu     1620

aaucagcggg ccauucagca augugagauc cuuggacugc ccaucaacgg cacaaauauu     1680

cucaaaauga agcuguggcu ggagcagggc gaaugcugug cguacagcgg ggagaaaauc     1740

accagagaac accuucucga uucuaacgcc cuucaggugg accauauuua uccuuacagc     1800

cgcuccuucg augacucuua uaugaauaag guccuggugu uugcuaaaga gaaccaggac     1860

aagggaaaua aaacacccuu cgaggccuuu ggcggggaua aggaaaagug ggaccggauc     1920

cucagccugu ccgcuaaacu uccuaagcaa aaacagagac ggauuagcaa caccaacuuc     1980

aaggauaaag agggaggcuu ccuggcccgc aaucucaacg acaccgggua caucgccaag     2040

cuggcuucuc aguggacaaa ggauugccug gaauuugagg accuuagaga aaaugagguu     2100

acgauugcgg gcgagaaagg auccagcgug cacaucgaag ucgugucugg ccagcucacc     2160

uccaugcugc ggcauuacug ggggcuggga aacaaggaua gaaauaacca ccuccaucac     2220

gccgucgacg cugugauccu ggccuauaca aauaacgcca ugauuaaggc uuucgccaau     2280

uuucgcaaaa accaagagga aaauaaaaag aaguucuacg cgaaacagau cgcugagagc     2340

gaauauaagg ccaaaaaggc cuuuuucgag cccuguaaaa acuuucggca gagaguugag     2400

gaaaagauua aggagaucuu cguguccaaa ccgccucgca agagaguccg gggcgcccuu     2460

caccaggaaa ccuuuuaccc cuucgagaaa cugugcaaug auaaggaaua uucugagaaa     2520

cucaagaugg acuacggggg agagaagggc augcaaaaag cuauugcgcu ggggaagauc     2580

cgccagaucg gcacaaaaau ugugagcaac ggagcuaugg uuagagugga uaucuuuaag     2640

gacaaaaagg gcaaauucua ugccgucccu auuuacacca uggauuuugc ccuggggauc     2700

cuucccaaua aggcuguggc cauuggaaag gacaaagacg gcgugaucaa ggauuggauc     2760

gaaauggacu ccaacuacga uuucuguuuc ucucucuuua aagacgaucu gauucugguu     2820

cagaagaaag agauggaacg ggcugagcuc ugcuauuucg ucagcuuugg guccacgaac     2880

gcccaaauca auguggagaa gcauaacaau aaguucgaag gacugagcga gaacgagaaa     2940

auucuuuacu cuaaugcgac acagaaggag guggccggca aauccaucgg gauucagaac     3000

cugaagaccu uugaaaaaua ucaggucagc ccccucggcg aggugcaaaa ggcugaguuc     3060

gaaccucacc aggagaucaa gcugaaaucu uccccgaagc gcaaagacug a              3111


<210>  3773
<211>  3111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3773
augcugcaca agaugcauua cagacucgcc cuggaccuug gcagcaccuc ccuggggugg       60

gcuaugaucc ggcucgaugc cgacaaccag cccugcgcgg ugauuaaagc uggaguccgc      120

aucuucucua auggcagaaa cccuaaggau gggagcuccc uggccguuac acggcgcgag      180

gccagagcua ugcggcgcag acgggaccgc cuucugaaaa gaaaggcccg gaugaugcgc      240

acgcucauug aauauggauu uuucccggcu gaggccgcgc aaagaaaagc ccuggaaacc      300

aucaaucccu uugcucugcg ggccaagggc cucgaugagg cucugacccc ugccgaguuc      360

gggcgcgcgc uuuuucacau uaaccagaga cggggauuca agucuaauag aaaaacagac      420

aagaaagaua acgacagcgg cgcccugaag acagcuaucg gcaaagugcg cgccaccauc      480

gaggcugagg ggugucggac agugggagaa uggcuccaua agagagaugu cgccggccug      540

accgugcgcg cccgguacca ccaggacaag guuauuaaag aggaugggaa gacgaaaauc      600

gacaaguauu acgaucuuua uauugacaga gcuaugaucg aacaggaguu ugacgagcug      660

uggaaaaagc aagcggaacu caaucccgcc cuguucaccg aggcugcccg cgccgaucug      720

aaagacgugc uccugcauca gcggccucuu aagcccguca aguccggaag augcacauuu      780

augccugaug aagaacgcgc uccgcuggcc cuccccucua cccagcgguu cagaauguac      840

caggaaguga acaaucugcg cauucuucgg gacggccuga aagaggagcc ucucacacug      900

aagcaaagag augaccugau cgcggcucuc gaaaagaaca gcaagcacuc cuuuacccag      960

auuaaaacgc ugcuuggggu uggaggcgcc gugcaguuca auuuugagga ucugaagcgg     1020

caggaacuca agggcaacac aaccagcgcc auccugucua augacaaaca cuucgggaag     1080

gcuugguuca cauuugauga ggccaaacaa gacgcuaucg uccuucagcu ggugaaggaa     1140

gagaacgagg ccaaacucgu gcgcuggcug caggaugaaa ccggaauuga cgagaagaga     1200

gcggaagcca ucgcuaaugu cggccugccc gagggguaug gaucccucug ugccaaggcu     1260

cuggcccgga uucuuccuga acugagacgc gacguuauga ccuacgauaa agcggugcaa     1320

gccgcuggcu ucgagcauca cagccggcuc gggucuaaca cagagauccc cggacugacg     1380

uuuaagauug aauccaucga ccaggauagc ggcgagguga aacaguucca uauccacaag     1440

guccugccgu acuauggcga auaccuucag agacacgugg gguuuggauc uggcgagccu     1500

gaggaccccg ccgaaaaacg cuaugggaag auugcuaacc cuaccguuca uaucggacuc     1560

aaucaaauua gacugguggu caacgcccug cucaagcggu acggccaccc cuccgaggug     1620

aucguugaac uggcccgcga ucuuaaacag agcaaggacc agagagauga ggaauccaaa     1680

cggcaggcug agaaucaaaa gcgcaacaaa agacugcggg acgcgauugc cgggacacuc     1740

aagaucucug aggaacgcgu gagacgggau gacauccaga aaaugauucu gugggaggaa     1800

cugagcuucg aucccgcuga ccgcagaugc ccuuauuccg gcgcccagau cagcgccacc     1860

augcuucucu cugaugaggu cgaaguggag cauauucugc cguuuuccca gacacuggac     1920

gacagccuca auaacaagac cgucgcucug cggcaagcca auagaaucaa gggaaaccgc     1980

acgcccuggg augcguucgg cgcucaggcc guggccgggu uugacuacga gucuauucuu     2040

guucgggcug aaggaaugcc uaaagccaag agauaucgcu ucgcggagaa uggcuaccag     2100

cgguggcuga aagaugacgc uagcuuucuc gccagagccc ugaacgauac acgccacaug     2160

uccaaggugg cuuucgaaua ccugaaacuu aucuguccca auacccgggu cauuccuggg     2220

agaaugaccg ccaugcuccg cgcuaaguuu ggacugaacg acgugcuggg ccucaauggg     2280

gagaagaacc ggaaugauca uagacaccac gccguugacg cgugcgugau cgccgucaca     2340

gaucagggcc ugcuucaacg guucgcuaaa gccucugcua gcgcccgcga acagcagcug     2400

aacagacucg uggagaacau gccccugccg uggggauccu aucgggagca ugugcaacgc     2460

gccaucaaug cuauuuggau cagccacaag ccugaccauu cucacgaagg cgcgaugcac     2520

aacgauaccg ccuacgggcu gagaggagac ggcaaaguuu ccuuccauaa gauggucgag     2580

ggggugcgga cacacagcga agauaaucuu aaagugauug agaucucuaa cgcuaaggcc     2640

acggacagac auggacuccu gcccaauggc gagccucgcc ccuauaaggg guacaaaggc     2700

gacuccaacu auuguauuga aaucguccgg aaugagaagg gaaaauggga aggcgaggug     2760

aucagcaccu uugaagccua ccagcugguu agagagcacg ggauugcucg ccuccggcau     2820

uccacacugu cugugagcgg aaaacuucug gucaugagac ucaugaucga ugacgugguu     2880

cgccugauua ucgaugagca gacccggaca augagaauug ccacccuguc cggcaacggg     2940

cagaucuuca uggcggacac gucugaagcu aauguggaug cccgcaacag aagcaaagag     3000

gaaaccuuug ccuaugucuc caagauggcu ggaagccuuc aaaaggccaa ugcgcggcgc     3060

aucaacauuu cuccuaucgg cgagcugaga gaccccgggu ucaaagaaug a              3111


<210>  3774
<211>  3114
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3774
auggagagaa ucuucggcuu ugacauuggg accacaagca ucggauucuc cgugauugau       60

uacucuagca cgcaguccgc cggcaacauc caacggcugg ggguccgcau uuuucccgaa      120

gcuagagacc cugauggaac cccgcucaau cagcagcggc gccagaagag aaugaugcgg      180

cgccaacuga gacggcgcag aauccggcgc aaagcccuua acgagacacu gcacgaagcg      240

ggcuuccucc ccgcuuaugg gucugccgac uggccuguug ugauggccga ugagcccuac      300

gaacugagac ggcgcggacu ugaggagggc cugagcgcuu augaauuugg cagagccauc      360

uaccaucucg cucagcaccg gcauuucaag gggagagagc uggaagaguc cgacaccccu      420

gaucccgacg uggaugacga gaaagaagcc gcgaaugagc gcgccgcuac acugaaggcc      480

cucaaaaacg aacagaccac acugggagcu uggcuugccc ggagaccucc gucugaucgc      540

aagcggggca uucacgcgca uagaaauguc guggccgagg aauuugagcg ccugugggag      600

guucagagca aguuccaccc cgcucucaaa uccgaagaga ugcgggccag aaucagcgac      660

accauuuuug cucaacgccc uguguucugg cggaagaaca cgcuggggga augcagauuu      720

augcccggag agccucuuug ucccaaaggc ucuuggcugu cccagcagcg ccggaugcuc      780

gaaaagcuga auaaccuggc caucgccggg ggaaaugcua gaccgcucga ugcggaggag      840

cgggacgcca uucugagcaa acuucagcaa caggcuucua uguccuggcc uggcguccgc      900

agcgcccuga aggcccucua uaagcagaga ggcgaacccg gggcugagaa aucucugaag      960

uucaaccuug aacugggagg cgaguccaaa cuccugggga augcccugga agcgaagcuc     1020

gcugacaugu uuggaccuga uuggcccgcc cacccccgga aacaagagau cagacaugcc     1080

gugcacgagc gccugugggc ugccgacuac ggcgaaaccc cugauaagaa acggguuauu     1140

auccuuagcg agaaggacag aaaggcucau cgcgaagccg cggccaacuc cuucguggcu     1200

gauuucggga ucacaggaga gcaggccgcu cagcugcagg cccucaaacu gccgaccggc     1260

ugggagcccu auucuauucc ugcgcuuaau cuguuucucg ccgaacugga gaagggcgaa     1320

agauucgggg cucuggucaa cggacccgac ugggagggcu ggcggcgcac aaauuuuccu     1380

cacagaaacc aacccaccgg ggaaauccuc gauaaacugc cgagcccugc cuccaaggag     1440

gagcgggaac gcauuagcca gcuuagaaac cccacggugg ugcggacaca gaaugagcug     1500

cgcaaagucg uuaacaaucu caucggacug uacggcaagc cugaccggau uagaaucgaa     1560

guggggcgcg augugggcaa gucuaaaaga gagcgggaag agauccaguc cggaauuaga     1620

cgcaacgaga agcaacggaa aaaggcuacc gaagaccuga ucaaaaaugg cauugccaac     1680

cccagcagag augacgucga gaaguggauc cuuuggaagg aagggcagga gcgcugcccu     1740

uacacaggag accagauugg cuucaaugcc cucuuucggg aagggagaua ugagguggag     1800

cacaucuggc cccgcucucg gagcuucgau aacuccccga gaaauaaaac ccugugucgc     1860

aaggacguua acaucgaaaa aggaaaucgg augccuuuug aggcuuucgg ccaugaugaa     1920

gacagauggu cugcgauuca gauccggcug caagggaugg ugagcgccaa gggcggaacc     1980

ggcauguccc ccgggaaagu caagcgcuuu cucgcuaaga caaugcccga ggauuucgcc     2040

gccagacagc ugaacgacac gcgguacgcu gccaaacaga uucuugcgca gcugaagcgc     2100

cucuggccug auaugggacc ggaggcuccc gugaaaguug aagccgugac cggccaaguc     2160

acagcccagc ugagaaagcu guggacccuu aauaacaucc ucgcugacga uggggagaaa     2220

acacgggccg accacagaca ucacgcgauu gaugcucuga ccguggccug cacgcauccu     2280

ggaaugacaa auaagcugag ccgcuauugg cagcuccggg acgaucccag agccgaaaaa     2340

ccugcucuga ccccgccuug ggacaccauc cgcgccgacg cugagaaggc cgucucugaa     2400

auugugguuu cccaccgggu gagaaagaaa gucagcggcc cccuucacaa ggagacaacc     2460

uacggggaua caggcacgga caucaaaacc aagucuggaa cauaucgcca auuugugacc     2520

cggaaaaaga ucgagucccu gagcaagggc gaacucgaug agauuagaga cccgcgcauc     2580

aaagaaauug uugcggccca uguggcuggg agaggaggcg auccuaagaa agccuuuccc     2640

ccuuaccccu gugucucccc cgggggaccu gagauccgga aggugcgccu gacaucuaaa     2700

cagcagcuga accuuauggc ucagaccggc aacggguaug ccgaccucgg cagcaaucac     2760

cauauugcca ucuacagacu gcccgaugga aaggcugacu ucgaaaucgu gucccuguuc     2820

gaugcgucuc ggcgccucgc ccaaagaaac ccuauuguuc agcggacgag agcugacggc     2880

gccagcuucg ucaugucccu ggccgcuggg gaggccauca ugauucccga gggaagcaag     2940

aaaggcaucu ggauugugca gggggugugg gcgucuggac aggucgugcu ugaacgcgau     3000

accgacgcug aucacuccac aaccacacgg cccaugccua auccgauccu gaaggacgac     3060

gccaaaaagg uuagcaucga ucccauuggc agagugcgcc cuucuaacga cuga           3114


<210>  3775
<211>  3117
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3775
auggccaaac aggugagaua ccggcugagc cucgaccugg gcuccaccuc ucuugggugg       60

gcuaucuucc gccuggaugc cgacgcgcac cccacagcua uuaucaaggc cggagucaga      120

auuuuuagcg auggccggaa cgccaaggac ggguccucuc ucgcuguuac gcgcagagcc      180

gcucggagca ugcgcagacg gcgcgauaga cugcuucggc gcaaaaccag aaugcuggac      240

gcccuccugg aguauggauu cuuuccucaa gaugcggccc agcggaaggc ucuggaacgc      300

cucaauccgu acgcccugag agcugagggc cuugcccggc cccugacacc uggggaguuc      360

gcgagagcca ucuuucaucu caaccagcgc cggggauuca aauccaauag aaagaccgac      420

aagaacgaua augacucugg cgcucugaaa acagccacca gccagcuucg ccaagauauu      480

caggcuuccg gcugccggac agugggggaa uggcugugga cccagagacu ccagaagaaa      540

cccgaaggag ugccucacca aggcguccgc gcccgguaua gagagacgcc ccauaucaac      600

agcgagggga agaaacgcau cgaaaagaag uacgaccugu auauugaucg gucuaugauc      660

gaggccgaau uugacgcucu gugguccgcg caggccaccu ucaauccugu gguuuuucac      720

gcuacagccc augagaaacu caaggacauu cugcuuuacc agagaccgcu gcgccccgug      780

aaaccuggac gguguacccu ccugcccgag gaagagagag ccccucuugc ucugcccagc      840

acacagcggu uccgcaucua ucaagaacuc aacaaucuga gacugcucgu cgaucagcug      900

aacgagcagc cgcuuacccu ggcccaacgg gacacgauug cgacacucau ggaaggcaga      960

gcuaagaucu cuuuugauca gauucgcaaa gcccugaaac uuggguccau gaccaaguuc     1020

aaucuggagg acgauaagcg gaacgagcuc aagggaaaug ccacaggcag ccagcuggcu     1080

aaaaaggaau uuuucggccc ugccuggcac caguucgacg aggcucugca agaugacauc     1140

guguggcacc uccugucuga agagaacgaa uccgcccuua ucagcuggcu gcaggauaga     1200

accgggauug acgcggccac cgcucaggcc guuuccaaug cuucucuccc cgagggauac     1260

ggcagccugu cccgcaaagc ccuggaggug auccuuccua gacuccggag cgaagucauu     1320

acauacgaua aggcggugca ggccgcuggg uuuucucauc acucccaacu gggauucgag     1380

uaugaccccg acgaugugga agacuggauc gaucaugcca cgggcgaggu caaacccauu     1440

uuuaagcgcc ugccuuacua ugggaaggcu cuccagagac acguugccuu cggaagcggc     1500

aaaccggaag acucucagga gaagcgguac ggcaaaaucg ccaaccccac cgugcacauc     1560

gggcugaacc aggugcgcgu cguggcuaau gcgcuuauug ccagauaugg acggccuaca     1620

gagaucauua ucgaacuggc ucgcgaucuc aagcaaagca gagagcagcg gcagcaggac     1680

caacgcagac aggccgauaa ccagaaacgg aaugcccgca uuagaaacuc caucgcucaa     1740

gcccugggca ucgcggaagc ucggguuaga cgcgaggaca uucagaagug gauccugugg     1800

gaggaacuug aucccgggga cgccaugcau cggugcuguc cuuacucugg agugcccauu     1860

agcgccucca ugcuccugag cgagcagguc gaaaucgagc acauucugcc guuuucuaug     1920

acccucgaug acucccugaa uaacagaaca gugagccuuc gccaggcuaa ucggaucaag     1980

agaaaccgcu cuccuuggga ugcccgggaa gacuucgagg cgcaaggcug gcauuaugac     2040

gggauucugc agagagcugg ccucaugccc guucagaaac gguaccgcuu ugccgccgau     2100

ggauaccaga gauggcugcg ggacgaucaa gacuuccugg cucgcgcccu uaaugauacc     2160

agauaucucu cccggaucgc ugccgaguac cugagacugg ugugcccuca gggcacgcgc     2220

gucauccccg ggcagcucac agcgcugcuu cggagacgcu uuggacugaa cgacauucuc     2280

ggccuggaag gggagaagaa ucggaacgau cacagacauc acgccgugga cgcuuguguc     2340

aucggaauua ccgaucacgg ccugaugcag cgcuucgccc gggcuagcgc ccaagccaga     2400

gaacaggggc uuacccgccu cgcugaggaa augccucugc ccuggccgac auauagagac     2460

cauguggauc gggcgguuuc ccugaucugg gugucucaca aaccugacca uggccacgag     2520

ggagccauga uggaggaaac cgcuuacggc cucaugaagg augggacaau ucgccagaga     2580

cacaaagccg acggaacgaa gggccgggag gucgccaaug ugauccgcau cgcugaaccc     2640

gcccaaccca ccagacaugg gguugacgau cagggacggc cucugccgua uaagggcuac     2700

auugggggca gcaacuauug caucgagauu acaagaggag cgcagggcaa augggaaggg     2760

accgugaucu ccacaucuga cgcuuacgga auuguccgcg cccggggcau cgccgggcuu     2820

agaaauacca gccagggaca aaacggccac ccccugguga ugcgccucgu gaucggggau     2880

ucccugcggc ugagaauuaa cgacgcccuu cagacgaugc ggguuaccaa gaucagcggc     2940

aauggacagg ucuuuauggc uggcgugcau gaggcgaacg uggaugcucg caauagagac     3000

aaaucucagg ccuucgcccu ggucuccaag auggcuggga gccuccaagc cgcucaggcc     3060

cggcaggcga caauuucucc uaucggagag auucgcgauc ccggcuuucc uggguga        3117


<210>  3776
<211>  3120
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3776
augaagaaaa gaauccuggg ccucgacauu gggaucgcca gcgugggaug ggcucugauu       60

gaguucgaug acgaauuuca ggauaacaag aaaaagggca ccaucauuaa auccgggguc      120

cggaucuuca cacgcgccga gacgcccaag gaaagagagc cucuugcgaa gccgcggcgc      180

gaagcuagau cucaacggcg cagaaucagc ugccggguug agcgccugaa uuuugugaga      240

gaacucuucg ugaaaaacgg acuggucgcc aaggaauccg ugaauaaaaa caaucccaac      300

aauauuuaca ucacccuuaa ccagauuaag acaccuuggc agcugcggau ggaggcccuc      360

gaccgcaaac ugaccaauga agaguuugcu guugugcuga cacaccucgc caagcauaga      420

ggcuauaagu cucugcggaa aaccgauagc aaggacaaca aaacagggga gaugcuuaag      480

gcuaucaauc agaacaaaga acgccuggau aauuccgacu acagaacccu cggagagaug      540

uucuguaagc acgaaucuua uaaagagcau aagcggaaca aggauggcga auacauuaau      600

agcgucacga gagagcugau cgaggaagag gccgaaaaga uuuuugcgaa gcaacgcaaa      660

gagggcaaca acuucgccga cguggauauc gaacgggagu aucuuaagau cgcuuuuucc      720

cagaaaccca ccgggagcau uaauaagaug guuggauucu gcacauuuga gaacggcgaa      780

aagagagccc cucgcgacuc uuacuccgcu gagcuguuca aagugcucaa uaccaucaac      840

aauacaacca uuacggauau gaagacaggg gaaacccgga acuuuacagg agccgagauc      900

aaaaagauua ucgaaaucau uaaagacacc aagggcauca ccuaugucaa gauuaaaaag      960

gagcugaaac uggggagcuc ucaguucucc ggaguggacu acagagagaa ugcgaaaagc     1020

aaaggcgaag gcgagaucuu cgugucuaug aagacauacc acgaaauuaa guccaaaauc     1080

gagaagcuca gcaaagagaa guggaacaaa cugaaggaaa auacggagcu ucuggauucc     1140

aucgucaaca uuaucgccaa uuuuaaagac gaugaaucua uuaagaagga guuccagaaa     1200

cucaagaucg acgaagauau uaucgacguu cugcuuaacu uugaguucaa aaaguuuauu     1260

aaucugagcu ucaaagcucu ccgcaagauc auccccuuuc aauuccaggg gcagaaguau     1320

auuaccgccu ccaugagcgu gggauacucu cugaaacagg agggcaucaa gaacaaauuu     1380

cugcgggugc cuucccaaga agagaauguc aacgugccgg uuguggcuag agccuucgcc     1440

cagacacgca aggucauuaa cgcugugauc cgggaauaug ggcaguuuga ucaaauuaau     1500

aucgagcucg cgaccgaccu gaaaaacagc aaguucgaua gaucuaagau cgaaaaagga     1560

cagaaggagu uucagagcca gaaagacaag guuuuccaag aggugcagga ucuuacaggc     1620

gaaauuaucc ccuccucuag ccugcuccug aaauaccgcc uuuggcggga gcaggacgaa     1680

agauguauuu auuccgggcg gaagaucagc cgcaaggacc ugcucgauac cggauuccug     1740

gacauugauc auaucauccc uuacucuaaa uccauggacg auggcuuuaa uaacaagguc     1800

cugugccucg ccgaggaaaa uagaaacaaa aagaaugaca uucccuucaa cuauuaccag     1860

cggaucggca gagauuggaa uuuucugauu agcaucauug cuucuaugaa aaacaugaag     1920

uacgugaaaa agaagcgccu ucugaaacaa gagcucuccg acaucgagau cgagagcuuc     1980

auugagcgga auaucaacga uacgagauau gccacacgcu acauuaagaa uuuucuggaa     2040

aacaaucugg aguucaaaga gaacgaauuu aucaaggcca aaguccaggc uagacacggg     2100

ggacuuaccu ccgugcuccg guauaacugg ggcauuucua agaaucgcga ggaaagcuac     2160

cugcaccaug ccaaggacgc gaucaccguu gcuuguucca cacaggggau ggugcaguau     2220

auuucuaaca ucagccaaaa auacgagaau ggaaaguaua aaaugaagca gaucaaagaa     2280

agaaccgaga accggauucc uaagcccugg gauuccuuca aggacgaugu cgagcgcagc     2340

aucaaugaca uuuuuguguc uuucgccccg agacacaaag uuacaggcuc cgcccauaag     2400

gaaacgaucu acagcaaaaa gcaccuggag aaaggguacg ugaccauuaa gaagucucuc     2460

gacaaaauca agcuguccga ucuuaaaaac aucccuugcg acgaaaauug ugauauuauc     2520

cggguccuga aggagcgccu cgaaaaauuu aacgacgaua gccugaaggc uuucgccgac     2580

cccgugcaca ugccuacaaa gugcucuaau aaaaagggcc ccauuaucag aagcgugaaa     2640

auuaaggaga acaauaaaac cggaaucgag guucggaagg gccuggucga acgcggggau     2700

auggugagag uggacgucuu uaucaaagau aagaaguauu accuugugcc cauuuauguu     2760

uccgacuuca aaaacaagga ucucccuaau aaagcuaucg ugagcucuaa guccgagaac     2820

gaauggauua ugaucgacaa aacauacagc uucaaguuuu cccuguucaa ggaugaccug     2880

auuaaaauca agaauaaaaa gggagagauu uuuggcuauc ucgggggagu ccaucggcug     2940

accggcggga ucaacaucaa aucuuguuac aagccgaagg aaaaagacgg caacauugga     3000

agcaagugcc uucuggauuu cagaaaauuu caggugggca uccucgggga cuauaaugag     3060

guuaagcacg agaaacgcau gcccgccuac auuaacacga agaaggauaa agaacauuga     3120


<210>  3777
<211>  3123
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3777
auggacagac gggaugcccg cgcuaugaga cggcgcagag accgguucca gcaacgccag       60

agagcccugc ucaaguaccu gguggcggau ggccuuuuuc ccgaggacga aggggagcgg      120

aaagcucugg ccgcccucga uccuuaugcu cugcgcgcca gagcucuuga cgaagcccug      180

agccuccacg agcugggacg ggcgcuguuc caucucaacc agcgcagagg cuuuaagucc      240

aaucggaaag ccgaucgcaa ggcugacgau gacgccggga aaaucgcuau uggagucgau      300

agagcccggg aagcgauggc cgagaacggc gcuagaaccu acggggagug gcugcaccgc      360

cggagagccu cugcuacaga cgccaaugcc aucccgcccg uucgcacgcg gcuuagaccu      420

gaaagcggag aggaugcuaa gggcgacggc uaugacuucu accccgggcg cgaucugcuc      480

gaagaggagu uugcggccau uugggaagcu caggccgccc aucacccuga cgugcugacc      540

gaugacgugu augagcggcu uuucgaaauc gucuuuuacc aaagaccccu gaaggcuccu      600

cagauuggaa ugugcacacu caucgccggc gaucgccggc ugccgaaagc gcauccccug      660

uuccagagac gccggcuccu ggaggaacuu aacgcucuga ugaucgugag accuggggag      720

guugcccagc ggcucacccg cgaggaaaga gacacacugu uucuuaagcu gaaagaucgg      780

aauaaggugu ccuucgaguc ucucagaaaa acccugaagc uggaccccga ugcccgcuuu      840

aacaaggaaa gcgagaaucg gacagaacuc aaaggagacg aggucgcugc cgcuaugggc      900

uccaagacca gauucgggaa ccgcugggug caccugucua uugaggaaca augggccguu      960

aucgagagac uuggagaugu ggaaagcgac gcggaggacg ccgcuuuucg ggccuggcug     1020

gcugaacgcu augagcucac gccugagcag accagagccg ucacaaaugc gccccugccg     1080

cagggcuacg gccgguucgg ggaaaccgcc acacgcaaac uuauugaugc ucugagagac     1140

ggagagaagg auggccgggu gcucguguac uccgaagccg ucaccgcugc cgggcuggga     1200

caccauagcg accgcagaac gggcgagguu uucgaggaug aagaggggcg gccucagcug     1260

cccuauuacg gaguggcccu cgaacgccac aucaugccug gcacaggcga ccccucugau     1320

uccgacgaga ugagaguggg gcggcugacc aaccccacag uccauauugg acuuaaucaa     1380

cugagacgca ucgugaaccg gcucauuaga gcuuauggcc cuccggcgga aaucgccauc     1440

gagcuggcuc gcgagcugaa acuuaccgau gaagacaaga aacggauuaa uaaggagaac     1500

aauagcaaca gacucgaugc cgaacgccgg ucugagaagc ugcuggccau cgggaaaccc     1560

gacaccggag cuaacagagc ccuccugaaa cuuugggagg aacugaaucc ugcgaacguu     1620

cucgaucgcc gguguauuua cuccggcgug cagaucagca uugacaugcu guuuucugcu     1680

gccgucgagg uggaccacau ccugcccuuc acagccacgc uugaugacuc caaugggaac     1740

aagaucguuu gcaccagaga agcuaaucgg gagaagcgca aaagaagccc uuaugaagcc     1800

uggggccacu ccgcgcggug ggaggaaauu gcugcccgcg ccgcucagcu ccccagaaac     1860

aagcggugga gauuugagcc ggaugccaug gagaaguuca aagacgaugg aggcuuucaa     1920

gcucgccauc ugaucgacac acaguaccug gcccggcucu cuagagaaua ucuggcgacc     1980

cuuuacccug auaaggggga gggaagcggc cacauuuggg ugucccccgg gcgccugaca     2040

gaaauggucc ggagaaaacu cggacugaau gaccugcuuc cugaucauaa cuucggcggg     2100

ggcgccgacc aggcuaagaa ucgccucgau caccggcauc acgccaucga cgcugugguc     2160

guggccauug uugauagagg aaugcugaac cgcaucgcca gagcuagcgg ccaggagggg     2220

gcggagggac gggaacgcau cauuaucccc gagccuuggg aaggcuuuag agacgaccug     2280

cggaccgccg ugaaugcuau uacggucucu caccgcacag aucaugggac cgccuccaaa     2340

gccggacucc ccaagggcaa ggacgcuacc gccgggagac ugcacaacga uacagcguac     2400

ggcuucaccg gagagagcga cgcuaaaggc aauucuaucg ugguucggag agugccgcuu     2460

agcuccuuua agaaacuggc cgauauugac cgcguccggg auccugaacu caaggccgcu     2520

cuguaucaac aucuggacgg gcuugaggga aaagccuucg agaaggcgau cggcgaauuu     2580

gggucuagag aguggcaccc cuacggaggc gugcgccggg ugagaguuac agaaccccuc     2640

agcgucauuc cuauccggga ucgcaacggg cacaucuaua agggcuacaa aggagacucc     2700

aauuauagau acgaugugug ggagcugccg ggcgggaagu gggugaacga agucgugagc     2760

auguucgacg cucaucagcc cggauggcag ucugccauuc gggccgcuaa cccuacggcc     2820

cgcaaaguuc ugucccucca cagagacgau gugcuggcua ucgagcggaa uggcgccgag     2880

cgcgaacuug ucagaguggu uaaguuuagc gagaaacagu ucgcgguggc ucccccuaac     2940

gaagggggag cccugaaggc ccgggacgcu gauaaaggcg accccuucaa guauucuuac     3000

ccuuccccca auacccucaa ggcccugcgc gccagacaag uccggauuga ugagcugggc     3060

agagugcuug acccgggguu uccugcucgc acaaaacagc ggaagaccag aaaacccaac     3120

uga                                                                   3123


<210>  3778
<211>  3132
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3778
augcagagag acaacccccu gguguaccgg cucgcccugg aucuuggcag caccucccug       60

gggugggcug ucuuccgccu cgacgccgag ggaucuccuc uggcgaucau uaaggcuggc      120

gccagaaucu uuagcgaugg gcggaauccg aaagacggau ccucucuugc cguuacacgc      180

agagcugccc gggcuaugcg cagacggcgc gauagacugc ucaagcggaa agcccgcaug      240

cugggcgcgc uggaaagaca cggguucuuu ccccaagacg agagcgcccg gaaggugcuc      300

gaaacgcuga acccuuuccu ucugcgcgcu cagggacucg agguggcccu gaaacccgaa      360

gaguuuggca gagcucuuuu ccaucugaau cagcggagag gcuuucaguc caaccgcaag      420

accgaucgga aggacucuga uagcggggcc cucaaacaag cgauuggaac acugagagcc      480

cagaugcacg aguccggcug ccgcaccguc ggggaauggu ucuggaaggu gcggauggag      540

cauaaaccug aaggaguuaa gggccagggg gugagagcuc gcuaucggga gacacccuuu      600

accacagacg agggcaaaaa gagaaucgau aagcgcuacg accuguaugu cgaucgggcc      660

augguggcuc aggaguucga cgcccucugg gccgcucaag cggccuuuaa uccugcucug      720

uucaacgagg ccgccagagc ugaacuucgc gacauucugc uccaccagcg gaaacugaga      780

ccgguucagc ccggacggug uacccuugug ccugaggaac cccgcgcccu gcucgcgcug      840

agcucuacgc agagauuucg gaucuaccaa gaggucaauc aucugagagu gcuccacgau      900

gagcugcgcg aagagacccu uacacuggcu cagcgggacc agaucguggc ccuccuggaa      960

aagcaagcca aagucaccuu cacagcuaug agaaaggccc uuggcuucuc cgcucuguuu     1020

aaccucgagg augccaaacg cgacgggcug aagggaaaug cgaccgccgc ugcccugagc     1080

aaaaaggauc ucuucggccc ucugugggcu ggguuuggaa cggcccuuca ggacgaaauu     1140

guuacacagc ugaugaccga ggagucugaa cccagacuca ucgcguggcu gcaggagcac     1200

acaggcgugg augaagcccg ggcucaagcc auugcuaaca ccgggcugcc ggagggauau     1260

ggcucccuug gccgcaccgc ccucgccaga aucgugccug aacugcagcg ggacgucauu     1320

acauacgaua aggcugugca ggcggccggg uucgcucauc acagcgaccu gggauuuucu     1380

uucgagcaug gcgccgauga gguugaacgc aucggggaga gaacgauugc cuccaccgga     1440

gaaaucaaac ccguguuugc uuucaagcag cucccuuauu acggccgggc ccugcaacgc     1500

cacgucgcgu uugcuaaaga caaucccaga aacgacgagg agcgguacgg gaagaucgcc     1560

aaucccacag ugcacauugg ccuuaaccag guucgcgugg ucgugaaugc ccugaugaga     1620

cgguauggaa gaccugcuga agucgugauc gagcucgccc gcgaucugaa acagagccgg     1680

gaacagaagc uggaggcgca acagagacag gcugacaacc agaagcgcaa cgcccggauu     1740

agacgcucca ucgccgaagu ucuuggcauu ucugaggagc gggugagaac ccucgauauc     1800

caaaaaugga uccuguggga agaacugagc caugacgcug ccgaucgccg gugcccguac     1860

uccggggucc agauuagcgc uucuaugcuc cugggagaaa gaguggagau cgaacacauu     1920

cuucccuucu ccaugacacu ggacgauagc cucaauaacc ggaccguugc ccugcgcgag     1980

gcgaauagaa ucaagcggaa ccgcgccccu ugggaggcua gagccgacuu ugaagcucag     2040

ggcuggucuu augaugggau ucugcaacgg gccgagagaa ugccccuucg caaacgguac     2100

agauucgccc cugacggaua ugaacgcugg cucggcgagg auaaggacuu ucuggcucgg     2160

gcgcugaaug auacgagaua ccucagccgc guggccgcug aauaucugcg gcuugucugu     2220

cccggguccg ccacaagagu gaucccgggc cagaugaccg cccugcuccg cggaaaauuc     2280

ggccugaacg acgugcuggg gcuugacgga gagaagaaua gaaacgauca ucggcaccau     2340

gcuguugacg ccugcgucau cggcgugacc gaucagcgca ugaugcagag auucgcgcaa     2400

gcuaaugccc aggcccggga ggacgggcuc acacgccugg uggaagauau gccucugccc     2460

uggccuaccu acagagacca cgucgagcgg gcugugcacc auauuugggu uucucacaag     2520

cccgaucaug gauuugaagg cgccaugaug gaggagacaa gcuacgggau ccucagagac     2580

ggcuccauua aacagcgccg gaaggcggau ggaagcgcug gcagagaaau cucuaaccug     2640

auucgcaucg ccgagccugg gcagccccaa cggcacggag uggacgccga aggcagaccg     2700

cuuccuuaua aaggguacgu cggaggcucc aauuauugua uugagaucac ggugaacgau     2760

aaggggaaau gggaaggcca gguuaucagc accuucgacg cuuaccggau ugugcgcgag     2820

ggaggccuga gacagcuccg gcaccccaca caggggcaaa auggacgcgc ccuggcuaug     2880

agacugguca ucggcgauuc ugugcggcuu gaggccgggg aaggaucccu gcagacccuc     2940

cgcguugcga agaugagcgc cgcuggccag guguuuaugu ccgaggucaa ccaagccaau     3000

guggacgcua gaaaccggaa cggcgaccug cccuaugugu cuaagauggc cgggagcuuc     3060

cagaaagccc gcgcuagaca ggucacaauu uccccuaucg gagaacuccg ggauccgggc     3120

uuuaaggggu ga                                                         3132


<210>  3779
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3779
augaaguaca cccugggccu cgaccugggg uauaacagca ucggaugggc cauucuugag       60

aaugaugaaa acggcucccc caucaaacug uucaaugcug gggugcacau uuuugagccu      120

ucuaucgaag acauugagau cgauggaaag ggcaaaacaa agaacgucaa aagacgggaa      180

gcccgccuca gacggcgcca gcuguucaga aagacgaagc ggcuugagaa acugcucaac      240

uuuuucaucc aagagggguu uuuccagaau cagaagguuc ugauuaauga cccguuugaa      300

cgcgcggagu uccuguacca gcucgauaaa aagagcagaa accccuauau ccugcggcgc      360

gaagcucuug acaauagacu gucccucgag gagauugccc ggaucuuuua ccauauugcc      420

caacaccgcg gauucaaauc uaccaagaga gaacuggcug agaugaacaa ggaugaaaag      480

aagaaaaagg aggacggcau cauucagagc gaaaucaaua cacuuaaaga ggagaucaag      540

aaaaacuccu gccggacccu gggcgaauau uuugccaaac ucuucgagaa uggggaaaag      600

auucgcggaa gacauacaga ucggaaaaug uacgaggaag aguuucagaa gaucuucaac      660

aaacagaagg aguucuaucc uaauauuuuu accgacgagu ucaaaaagaa gcuguacaaa      720

cugaucuuuc accaaagaga acucaagucu cagaaagaua aggugggcuu cuguagccug      780

gaacgcggga aaaagcgggc uccccuugcc cugcucgugu cccagagaua ccgcuauaug      840

ugcaagguca acaaccugaa ugugauugac acagagaccg gagaaaaccg ggagcuuacg      900

guugaugaga aaaagaaacu guacgaaaag cucgagaaua gcgaaaaacu gucuuuuaag      960

gagaucaaga aaauccugaa ccucuccgag aagagauaua uuuucaaucu ggaaaagagc     1020

gagaaguaca ucauuggcaa caaaaccaau gugaagauga aaaacaucau uaaggacugg     1080

gacaauuuug augaagacaa gaaaaacuuc uuuaucaaug aggcgcgcac agucgugaag     1140

aucgaaaccc uugagcggag agggaaaaag cugggacucu cugaugagga auccaaagag     1200

uucagcaagg uuguguuuga agacggcuau cugaacuucu cuaagaaagc cgucgagaaa     1260

cuucugccuu accucaaaga aggcaagaaa gaguaugagg cuauuaagcu ggccuacccc     1320

gaaaauuccg ugcagaacga uaaucugaag gagcucgacu uucugccucc gaucaaaagc     1380

aaggaacuua aaaaguacuu cgaggugcgc aaccccauug ucgaaaaagc ucugacagag     1440

acccgguauc ucguuaacuu ccugaucaag aaguacggga aaccugagac gauuaagauc     1500

gaacuggcca gagagcuuaa acucuccaau aagcaaaaag aacgcaagca uaaagagaac     1560

caggagaauc ggaagaagag agaagaggug auugaaaaac ugaagaacga ucugaaaauc     1620

ucugagccca cagaaaccca gauccaaaag uaucuccugg cggaggagug ugacugcaaa     1680

uguccuuaca caggacggag cauuuccaug gauagccuuu uuggcgaaca cccccuguuc     1740

gacaucgagc acauuauccc guuugaucgc ucucucgaca auuccuuccu gaacaagacc     1800

cugugcuaua gcgaagagaa uagaaaggug aaacauaaca agaccccuua cgaagccuau     1860

uacgggacag agaaauggga ggcuauuauc gaacggguca agaaauuuaa gucuaaugug     1920

agaaacgaga aacuucgccg guuccagaug acgaaagaag acguugagaa uuacauccag     1980

aacuuuagca cccagaaucu caacgauaca aaguaugccu ccaaacuggc uaagaaauac     2040

cugggacucc uguauggcuc uauuaaugaa gacgggaucg auaagaacag aaagcgcaga     2100

auuuucgcca gcuccggaaa agugaccagc acacuucggu acgcccuggg ccucaacacc     2160

auccugaaug acgagaacau uaagacgcgc gaggaucaca gacaucacgc uaucgacgcg     2220

aucacaauug cccugaccga uucuuccaug aucaaaaaga uuagcgacgc uucuaaacgg     2280

gaaaagaaua ucaacucccu uuuuaaggau uuccaagccc ccugggagaa uuuucgcaaa     2340

gaagucgagg agauuaucgc caagaugaac aucagcauua gaaucaauaa aaaggugucc     2400

ggccagauuc acgacgaaac cuucuauaaa aagccuuuua aagaggaaga gaagucuuac     2460

acacaucagc ggaucaaccu cgaggaacug accauuaagg augaggacaa aaagaaacag     2520

agcuccaagc ugaucgauaa uauuguugac ucuaaaaucc gcaacaucau uaucaauagc     2580

cucaacgggg aaaagcccaa gauuagauuc aaagacccca aggaucaccc uuauaucauu     2640

caaaagaaug gaaagaaaau ccagauccau aaggugacaa uuaagacgaa acuggagacc     2700

uacaacgaaa ucggcccgga gaaggcuaaa cgguacguca aguccagcga cucucaccau     2760

auugaaaucu ucgaggaucu uaaaacaaag aagugggacg gguauguggu cucccugcuc     2820

gaggccuacg aacgcaaaaa gaaaaagcug cccauuguga auaaaaaccu gggagagggc     2880

aagaaauuuc uuuucagccu cgcgaagggg gaaaucaucg agcuggauac cgaaaacggc     2940

aagagagagc uguuuguugu gcggauuauc ucugcuucca auaaccagau uaaauucaca     3000

agaaucaaug acagccgcaa gauuaccgau aucccugcca cgggacucac cgccuguccc     3060

ucuagccuga aaaagcggaa cugcaaaaag gucgugauua auauccuugg cgagaagcac     3120

uauuccaacg acuga                                                      3135


<210>  3780
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3780
auggccgaga agaaauucaa guacagacug ggccucgacc uggggaccaa cagcaucgga       60

uggugcaugc uucggcugaa uaaagauaac gaacccgcug ccgugauuaa guccggcguc      120

cgcaucuuuc acgacgggag aaaugagaaa acaaaggaac cucucgcggu ugugcggcgc      180

aacgcuagau cuauucggcg caaucuggau agaaagauca gccggcgcaa ccaucuucug      240

aauacgcucg ugaaauauuu ccugcugccg uccaacgaga uugaaagaaa ggcccucgcc      300

guccugaauc ccuacgagcu ucggucucgc gcugugaacg agagacugga acucuuugag      360

cugggacggc uucugaugca ccucagcaaa cgcagaggcu ucaaguccaa ucggaaaguu      420

gacaaggaag auucuagcgg gaagaucaaa ccugccaucg agagacugaa gaacaaaaug      480

caggagacca auaagaaaac agcuggagaa uaucuguacu cccucaugca aaacggcaag      540

cccgugcgcg cccggcuggg cagaauugac gggagcaaug gauaugagau cuaccuugau      600

cgcgcgcuga uugaagagga auauaaccuc aucaugaacg agcagaaaaa gcaucacaag      660

gagcugaccg acgauauuau caaugaaauu uuuaacauca ucuucuacca gcggccucuu      720

aaaaagcaga uuaucggcaa auguucucug acaggggagg acaccaagcu caaaaaggcc      780

cauguccugg cucaagauuu uauucugcuc cagaaggugc aggaccugag aguuuucgau      840

gacaaucagc uugaauuucg cccccugucc aacgacgaga aagauaucau uaagcgggaa      900

cucagaaaga agaaagaggu gagcuacgag aauauccgca agcugcuuaa gaaauauuac      960

gaaaacguca aguauggaau guucucucac gagacacugu acaaaaagcu ccaaggcaau     1020

aaaaccaacg cccugauguc cgacaaggaa aucauugggg auagcuggua uucucugucc     1080

ccugaggugc aguacgacau cauugaagaa cucuauagcg auaaggacaa ugaugagcug     1140

uccaaacuuu acucugaaaa guuuaaccug aaugagagcc agaucaacgg acuccugaau     1200

aaagcuauuu accggcugga cuccggcuau aucagauacg gcaagacggc cgugaccaac     1260

cuuaauagca ucauggauaa agacaaccuc aaccugcacg augcgcgcga agaggccggg     1320

uaugagaagg aaaauaaaca ggacgcuucu gaguaccugg aauauuacgg aaagguccuc     1380

ccggacuccg uuguggaucc ccaaauugag aacccuaaga augacgagga gcgguauggc     1440

aaaaucgcca accccacagu gcaugcugcc cugaaucagc uuagaaagcu ggucaacgag     1500

cucauuaaac uguacgggaa gccugaacag aucgugauug agcuggcccg ggaucuuaaa     1560

aauagccgca aggcuaagga cguuaucaac aaaaagauug cggaaaauag aaaacucaac     1620

gaggaagugg auaagacccu gcaagaguac ucugagaaau auaauacaca caucgucaag     1680

aacaagcuga aucgggacaa agugaagcuc uggagagaac ugggaaaaga uccccuugag     1740

cgcaagugca ucuacaccgg cgaacagauu aacaucgcca aacuguucag cgacgagguu     1800

cagauugaac auaucgugcc guauuccaag acgcucgaug acucuuuuaa uaacaagaca     1860

cugagcauga aacaggcuaa cuacuauaag gggaauaaaa ccccuuacga ggccuucaag     1920

gauuccaaag acggauauaa cuacgaugac auuaucgccc gggcuaagcu gcuuaauaac     1980

gacaauaaau ucaagagauu uacaaaggau gccauggaca ucuacaaccg cgauggcaaa     2040

gacuucauug cgagacaacu cagcgauacc caguaucugu cuacccgggc ucugaucuac     2100

cuccaguccc uguaugagaa ggcccaccac agcucuauuu ggacaauccc cggccagcuu     2160

acggcccuga uucgcaauua ccucgggcug aacacccuga ucuccaauga caaaaacaag     2220

aauagaaacg aucaucggca ccaugcuguc gacgccuuug uggucgcugu gacaagccgc     2280

ucccuucucc agaaaaucuc uuaugccgcg agccugcaag aagaucugga caauagaaac     2340

guuuccucuu accggaauaa gcuccuggag aagaugccug aacccuuccc caacuaucgc     2400

aaaagccuug agcuggccau ugauaacauc aagaccuccc acaaagcuga cagaagcauu     2460

ucuggaaagc uccaugagga uacagccuac ggcaucgugg aaaaugacga caaauacaac     2520

gucgugaccc gguggacggu ugauaaguuu aaggagcaga aacacuucga aacaauucgc     2580

gacaaugagc ugaaggaaau cgcuuguucc gauaaagagc uguuugccaa gaucguggag     2640

accagaaaaa uuaagagcau ccggaaacuu gcgaaagaaa acccugucau uaagaucaaa     2700

gacaagucug ggagagauua uaaagccuuc gcuggaggca auaacauuug cguggagauc     2760

uacgaagugg acgggguucg caaguccgag gucauucagc uguuugaugc cgcucaaaag     2820

ggcuucaugc cggaauggau gaaaaaguau cccaauggaa aacuccugau gcggcucuuu     2880

aagggcgacg ugaucggguu caucgagaac agaaaauaca auuauuacac cauuaaggga     2940

aucagcauug ccgaucagaa ccugaaacuu cuggccauca auaaggcuga gggcauuuuu     3000

aagaaagggc ucaacauccu guucgacucu aauaacaaga auagccugaa cgcgaaacag     3060

uucaauaucu ccgugacagg aauugucacc aagaaaaaga cagccgaugu gaacuuuugg     3120

aagaaccgca aauga                                                      3135


<210>  3781
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3781
augcugcaca agaugagaua ccggcucgcc cuggaccuug gcagcaccuc ccuggggugg       60

gcuauggugc gccucaacgc caaucagcaa cccugcgcgg ucaucaaagc uggaguuaga      120

auuuucucua acggccggaa uccuaaggau gggagcuccc uggccgugac acgcagagag      180

gcccggucua ugcgcagacg gcgcgacaga cuucugaaac ggaaggcucg caugaugaga      240

acgcucaucg aauauggauu uuucccggcc aacgagaccc agcggaaagc ucuggaaaau      300

cugaaccccu acgcccuccg cgcgaagggc cuggaugagg cccuuacacc uagcgaauuu      360

gcuagagccc uguuccauau uaaucagcgg agaggguuua aguccaaccg caaaaccgac      420

aagaaagauu cugacagcgg agcucucaag acagccauca aacagcugca cuccgugcuu      480

gauccccaag gcaaugacgg caagccucgg accgucgggg agcugcucua uaagagauuc      540

acagaucuga gcaaacugcc caaggaccgc accgugcggg cgagauaccg ccaggauaaa      600

auuguuaagg acgauggaaa aacgaagauc gacaaauauu acgaccucua uaucgaucgg      660

gccaugauug agcaggaauu ugacgcucug uggaagaagc agucugagcu uaacccuauc      720

cuguucaccg aaaaugccag agcugaucuc aaagacgugc ugcuuuacca acgcucccug      780

aagccgguca aacccggccg guguacauuu augagcgagg aggaaagagc cccucucgcc      840

cugcccucua cccagcgcuu ccggauguac caggagguga acaaucugcg gauucucaga      900

gaagggcuga aggaggaauc ccuuacacug cagcaacgcg augaccucau caacgcucug      960

gagaaaaaua acagacggac cuuuacgcag auuaagaagc ugcugggagu uggcgggagc     1020

gugcaguuca acuuugagga uccuaaaaga gaggaacuca agggaaauac aaccucugcg     1080

auccugggca aauccgaaca uuucggcgag gccugguucg cuuuuaacga agccaagcaa     1140

gacgccauug uccugcagcu caucaaagag gagaaugaag cuaagcuggu gcgcuggcuu     1200

caggaugaga cagggaucga cgaaaagcgg gccgagguga uugcgaacgc uggacugccc     1260

gaaggcuaug ggagccucug caucgaggcc cuggcccgca uucugccgga gcuuagaaga     1320

gaugucguua ccuacgacaa agcugugcag gccgcuggau ucgaacacca uuccaagcuc     1380

aaucggaacg aggaaauccc ugacauuacc uuuaaaaucg agucuaucga ucgcaauagc     1440

ggcgagauua agcaauucca ccuucacaaa gaacugcccu auuacgggga guaucuccag     1500

agacaugugg gauuuggcuc cggcaagccu gaagacagca ucgagaagcg guacgggaaa     1560

auugccaacc ccacagucca caucggacug aaucaggugc gcguuguggu caacgcgcuu     1620

auuaagagau auggccaucc cucugaaauc aucguggagg uugcccggga ucugaaacag     1680

uccaaggacc gcagagauga ggaaaauaaa cggcaagcug agaaccagaa gcgcaaugaa     1740

agacuccgga aagacauugc cgauauccug gggauuagcg aggaaagagu gcgccgggac     1800

gauaucgaga agaugauucu gugggaggaa cuuucuuucg acccugcuga uagacgcugu     1860

ccguacagcg gaguccaggu guccacgguc augcuccugu cugacgaggu ggaaguugag     1920

cacauccugc ccuuuagcca gacccucgau gacucccuga acaauaagac aguggcccuu     1980

cggcaagcga acagaauuaa aggcaaccgc accccuuggg aagccuucgg gacaagcaau     2040

aucucuggcu uugacuacuc cggaauccug acccgggcug agcucaugcc caaggccaaa     2100

agauaucgcu ucgcugagga uggcuaccag cgguggcuga aggacgaugc cggguuucug     2160

gccagagcuc uuaacgacac gcggcaccuc agcaaaauug cgcgcgaaua uaugucucug     2220

aucugcccua auacaagagu cauucccgga cagaugaccg ccaugcugcg gcgcaacuuc     2280

ggccucaaug augugcuggg gcuuaacgga gagaagaaua gaaacgacca ucggcaccau     2340

gcuguugaug ccugugugau cgccgucacc gaccagggcc ugcuccaaag auuugcugcc     2400

gcguccgcua gcgcccgcga acggcagcug aauagacugg uggagaacau gccgcuuccu     2460

ugggaguccu accgcgaaca cgugcggaga gccauugaug ggaucugggu uucucaucgc     2520

cccgaccaca gccacgaggg cgcuaugcau aaugauacag ccuauggacu ccggggcaac     2580

gggagagucu ccuuccacaa gaugguggaa ggaaaacgcg aguacaucga agacaaucug     2640

aaggugauug agaucaccga uacaaaagcg auugacagac auggccugcu cccuacgggg     2700

aagccccggc cuuauaaagg auacaagggc gacucuaacu acugcaucga gauuguccgc     2760

aaugaaaagg ggagauggga gggcgaaauc aucagcaccu ucgaugcuua ucagcuggug     2820

cggaaauacg gaauuguuca acuucgcuau cccgugcugu ccgucagcgg caagccgcuc     2880

gugaugagac ugauccggaa cgacuuuguu agacuggagc acgaagggau uacacgcacc     2940

cuucgggugu guaaaaugag agaggaugga caggucgccc uggccgacgu gacagaggcu     3000

aacguugaug cccgcacccg gacgaaggaa aucugcuacg uguucaaaac cggcgggucu     3060

cuccagaagg cuaaggccag acggauuaca aucuccccua ucggagagcu gcgcgacccc     3120

ggcuuuaaag aauga                                                      3135


<210>  3782
<211>  3138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3782
augcagaagg agaaaccccu ggccuacaga cucgcucugg accuuggcag caccucccug       60

gggugggccg ugcuccggcu gucugcgccu ggagaaccga gcgcugucau ccgcgccggc      120

uccagaauuu ucucugaugg gcggaacccc aaggacggaa gcucccuugc cguuacacgc      180

agagcugccc gggcuaugcg cagacggcgc gauagacugc ucaaacggaa ggcccgcaug      240

cuggcggccc uggagagaca cggcuuuuuc ccucaagacg aagcugcccg gaaagcucuc      300

gagaagcuga aucccuaugg gcuucgcgcc agaggacugg aaucugcgcu caagccugag      360

gaguuuggcc gggcccuguu ccaucuuaac cagagacgcg gcuuucagag caaucggaaa      420

acggauagaa aggacuccga uagcggggcu cugaaacagg ccaucggaac ccuccgcgcu      480

caaaugcacg aaucuggcug ccggacagug ggggaguggu ucuggaaggu gagaauggaa      540

cgcaaacccg agggagucaa gggccagggg gugcgggcca gauaccgcga gaccccuuuu      600

acaaccgacg aaggaaagaa acggauugau aagagauaug accuguacgu ugaucgcgcc      660

augguggcuc aggaguucga cgcgcugugg gccgcucagg ccacauuugc cccggcucuc      720

uucaacgaag ccgcgcgggc ugagcugaga gauauccuuc ugcaucaacg gaaacuccgc      780

cccguccagc cuggcagaug uacccuggug cccgaagaac cucgggcccu ucuggcccuc      840

cccuccacgc agagauuucg cauuuaucag gagguuaauc aucugcgggu gcugcacgac      900

gaacucagag aggaagcucu gacccuugcc caacgcgacc agaucgucgc ucugcucgag      960

agacaggcca aggugacauu caccgcgaug cggaaagccc ugggcuuuag cgcucuuuuc     1020

aaccuggaag augccaagcg cgacgggcuc aaaggaaaug cuacaaccgc cgcgcugggc     1080

aagaaggauc uguucgggcc gcucugggcc ggauuugaca cggcucugca agaugagauc     1140

gugacacagc uuaugaccga ggaaucugag ccuagacuga uugccuggcu ccaggaacac     1200

acaggcgucg acgaggcucg ggcccaggcc aucgcuaacg cggggcugcc cgaaggauac     1260

ggcucccugg gccgcaccgc ccuugcuaga auuguuccug agcuccaacg ggaugugauc     1320

accuaugaca aagccgugca ggccgcuggg uucgcccacc auagcgaucu gggauuuucu     1380

uucgagcacg acgcggauga agucgagcgc auuggcgaaa gaacaaucgc uuccacgggg     1440

gagauuaagc ccguguuugc cuucaaacag cugcccuacu acggacgggc ccuccagcgc     1500

cauguugcuu uugccaagga gaauccuaga aacgacgagg agcgguaugg caaaaucgcg     1560

aauccgaccg ugcacaucgg gcugaaccaa gucagagugg uugugaaugc ucuuaugugc     1620

cgcuacggcc ggcccacaga agucgugauu gagcuggcca gagaccucaa gcagagccgc     1680

gaacagaagc uggaggccca gcaacggcag gcugauaacc agaaaagaaa ugcccgcauc     1740

cggagaucca uugcugaggu ccugggaauc ucugaggagc gcgugcggac ccuugacauu     1800

cagaagugga uccucuggga ggaacugagc cacgaugccg cggacagacg guguccuuau     1860

uccggcguuc aaaucagcgc cucuaugcug cucggggaac gcguggagau ugagcauauc     1920

cugcccuucu ccaugacacu ugaugacagc cugaacaaca gaaccgucgc ucuccgggaa     1980

gccaaucgca uuaaaagaaa ccggacgccu ugggaugcua gaacagacuu ugaggcccac     2040

ggauggucuu acgauggcau ccugcagcgc gccgaacgga ugccccagag aaagcgccau     2100

cgguucgcuc cggacgggua ugagagaugg cugggagaag auaaagacuu ucuugcgcgc     2160

gcccucaaug auacccggua ccugagcaga guggcugccg aguaucugcg ccucguuugc     2220

ccuggcuccg ccaccagagu gauucccggg caaaugacag cucugcuucg gggcaaguuc     2280

ggacugaacg acguccucgg ccuggacggg gagaagaauc gcaacgauca cagacaucac     2340

gccguggacg cgugugugau cggaguuacc gaucagcgga ugaugcagcg cuuugcucag     2400

gccaaugccc aagcuagaga agacggccug acacggcuug ucgaggauau gccucucccc     2460

uggccuacgu acagagccca cguggaacgc gcggugcggc auaucugggu cucucacaaa     2520

cccgaccaug gguucgaggg agcuaugaug gaggaaacca gcuacggcau ucugagagau     2580

gggacaauca agcagcgccg gaaagccgac ggcuccgccg gaagagagau uagccggcug     2640

auccgcauua ccgaaccguc ucagccucag agacacggcg uggaugcuga ggggcggccc     2700

cuccccuaua agggauacgu uggcgggucc aacuauugcc uggaaaucac agugaaugac     2760

aaaggaaagu gggagggcca agucauuagc accuucgaug ccuaccgcau cgugagagaa     2820

gggggccuuc ggcagcugcg ccacucuacg cagggacaga acggccaagc ucucgccaug     2880

agacugguua ucggggacuc cgugcggcug gaagucgaug agcaggaacg cgugaugaga     2940

auuguuaaga ucaauggaaa caauggccag guguuuaugg cgccugucca ugaggccaac     3000

guggacgcuc ggaauagcga caaaaacgaa gccuucgcuu auaccuccaa gauugccggg     3060

ucuuuucaga aaacaaaggc cagacaagug accaucagcc cgauuggaga gcuucgcgau     3120

cccggcuuca aaggcuga                                                   3138


<210>  3783
<211>  3138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3783
augaagccca gaaaccugaa uuacauccuc ggccuggacc uugggauugc cagcguggga       60

ugguccgucg uugagaucga ugaaaacgag uauccuauuc ggcugaucga cgugggcgug      120

cgcaccuucg aaagagcuga ggucccgaaa acaggggaau cucucgcccu ggcgcggcgc      180

cuugcuagaa gcacgcggcg ccugauuaga cggcgcgccu uuagacuccu gaaggccaaa      240

cggcugcuca agcaccauca gaucgugaau gcugaggagc ugacccaacu ucccaaccag      300

ugcugggaac ugcgcguuaa aggacucgau ucccugcuuu cuaauacaga gugggccgcu      360

gugcugcucc aucugcugaa gcacagaggc uaccucagcc agcggaagaa cgaagcccag      420

aaugcggaca aagagcuggg gaagcuccgc gagggaaugg auaacaauuc caaacugcuc      480

cuggaaaaca auuauagaac cccugccgac aucgcuguca agaaauucgc cguggaggaa      540

ggccacaugc ggaaccaaag aggcgcuuac acacauaccu uuaaucgccu ugauauucug      600

gccgagaugc agcuccuguu caagacacag cgggaacugg ggucuaccua ugcgaacgga      660

gagcucgagc aggccuuuug ugaacugcuu cuguggcaaa agcaggcucu caauagaagc      720

cagaugcugu cccuuguugg caaaugcacg uucgagaagg aagagaaacg cgccgcuaag      780

gccagcuacu cugccgaacg guuuguggcu auccagaaac ugcaaaaccu ccgcauucug      840

gagaacgggg aggaaagagg acuguucgac uccgaguuua gccuccugcu ugaaaaugcg      900

uauaaccuga agucuggccu caccuacaaa cagguccgga agauccuguc ccugagcgag      960

aaugccauuu ucaaggggcu ucccuaccuc ucugaugacc uggaaaaacc ugagaagaca     1020

caguuucugg cuuucaaauu uuaucaccaa cucgccgaua uccugaagaa caauggauuc     1080

uccgacgagu ggcagaaacu uagccaggaa cccacccugc ucgauaagcu gggcacagag     1140

cugucccuuu guaaggaaga gaacgaguuc auugcccagu uuaauggcga acucccugag     1200

gcuaugcugu cuacccuguu caaccacacg aauuuugaca aauucaucca uaucagccuc     1260

aaggcccuga acaauauucu uccgcugaug gaacaaggga acgacuacac aaaagcgugg     1320

agaaaggugu aucccgagcc uaccaaaaag gaugaaaaga cacuuccccc uauccccgcu     1380

gacgagauuc gcaauccggu gguccugcgg ucccugagcc aggccagaaa aguuaucaac     1440

gccgugauuc gccuuuacgg aucuccugcu cggauccaca ucgagaccgc cagagaacuc     1500

ggcaaguccu augaugaccg gcagaaaauu aagaaacagc aagauaagaa uagcgacgag     1560

cgcgaucagg cugugaagaa auuucuggaa gagugcccca acuucgccaa caaggucaaa     1620

gggaaggaca uccugaaaau uagacucuac aucaaucagg auggaaagug ucuguauucu     1680

ggcaaaccuc uugaccccca ucggcugcuc gaaauugggu acguggagau cgaucacgcg     1740

cugcccuuua gcagaaccug ggacgacucc cagaacaaua agguucuggu gcuugccaac     1800

gagaaucaaa acaagggaaa ucagacaccu uucgaauggc ucggcaaaga ugagcaccag     1860

ugggcucugu uugucgcccg cgugaacggc ugccgguucc cguacgcuaa gaaacagaga     1920

auccugacga agaaacucga cgaacaaggg uuucugaagc gcaaucuuaa cgauaccaga     1980

uauguuucug ccuaccugau gaagcauauu aaagagaauc uccaccuggu gggaaagggc     2040

agcgacaaag ucuucgcguc caacggacag gugacaaauu uucugcggcg cuguugggga     2100

cuugaaaaga aaagagagga gggcgaucgg caucacgccc uggacgcuau cgucguggcc     2160

ugcagcaccg cuucuaugcg ccagaagauu acacucuuca agaaauauca aagauggaac     2220

cugaagaccg ggaaacauau cgaucaggaa acgggcgaga uuaucccccu ccacuucccu     2280

gcccccuggg acuuuuuccg gcaggaaguu augauucgca ucuuuuccga gaugccucag     2340

gaggaucuga ucaugcaacu ucccgacaga ccgcaggcca aucacgaguu cgugcagccu     2400

cuguuuguca gccgggcucc cucucgcaag auguccggac agggccauga agcgaaacuc     2460

agaagcgccc ggaugcugga agcuacaggg aaguccauua agaaagaguu ccugaccgau     2520

cuuaccauca agaaccugga acacauggug aacaaagaga gagagccuga acucuaccaa     2580

gcccugguug agcauuuuaa gaaauaugga gacaagccca aagaaaaguu cuuuaagaaa     2640

ggcggggugg aggucaaguc uauucgcauc agcaaaacac agaauaaguc cgugaaccuc     2700

ggaaauaaaa ccauugccga uaacggcgac aucgugcgga cagaccuguu ccuuaagaau     2760

aagaaauacu auuuuguuaa caucuacgcu uggcaggucu cuaaggggau ucugccuaaa     2820

gaaacgacca caggcaauau ccucgauaac agcuacgagu uccaguuuuc ccuguucaag     2880

aaugaccugc uugagauucc ccacccgaaa aacgaaaaug auagcauccu ggccuauuuc     2940

auuagaccug acgaugagcg ccgguggauc cucaaguuuc acgacaacgc gaagaucccc     3000

gauauuuacg ggaaaaagga cgaaaccucu aucagacugu ccauucaagg ccagaaauuc     3060

aucaagaaau aucaggugga ugagcucggg aagaauauuc gccccugucg gccuacaaag     3120

agacaaggag ugcgcuga                                                   3138


<210>  3784
<211>  3141
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3784
augaagaucu acagcuucga cauuggcgug gccuccaucg ggugggcuuu uauugagaac       60

gaaguccugc aggauugcgg aaucagaauu uucaccaaag ccgagaaucc caagaacggc      120

gcgucucucg cucugccucg gcgcgaagcc agagggguuc ggaaacgccu ugccagacgg      180

cgcggaagac ugaauacaau caagcaacuc cuguguaaag aguuuggccu ucagcuggaa      240

gacuaucuca gcaacgaugg ggagcugccg aaggcuuaca cggccuccaa ggcuaauccc      300

cugaaaucuc cuuaugagcu ccggacccug gcccuuagcc agaagcugga cuccaaagau      360

cucgugcgcg ugauccugca cauugcgaag cauagaggau acggcaacaa acacgccaag      420

gacucuaagg auacagaaag cggcaaaguc aagaaagcua ucgaguucaa ucggaagacc      480

cuugccgaaa aaggguaucg cuccguggga gaguaccugu auagagaguu uuuccagcaa      540

agccggauuu cucagaacaa uggcgcuaca gaauuuguua acgugcgcaa uaagaccggg      600

aacuacgagc auugcgucgc ccaggacaug cucaaagaug aacuggagcu gaucuucaga      660

aagcagaagg aacucggacu gcaccuuucc gacggcuuug agaaacaacu gcucgauaag      720

auuuucgagc agcggccccu gaaauccuuu gcggacaagg uggggaaaug ucaguucauc      780

gccggagcua agagagcccc uaaggauucu auuuccgcua ucgaauuugu ugcccuuagc      840

cgcaucauua auacacuggc caaccucucu aaaaaguccg gcgagaugua cgacaaagcu      900

auggugcuga ccauccugga cagcguccuc gaaaagggcg agauguccua uuacgcgcug      960

cgggaagcca uuaaucuuga ugagaaaauc agauucgcug acucucgccu ggauuauagc     1020

aaggagauua aggaaacgga gaaagugaag uucguggaau gcaaaaaccu caaguccuuu     1080

aaaaaggucc uggggaacug uuacaagacc cuugagcgga aacauaucga cagcaucgcc     1140

gaaagaauug ccguuaucaa ggauguggag aaacugcaca aggagcucga aucuuauugc     1200

gcuacagagc agcugucccu gaccagcgaa caaauucagg cccucucuca ccugaauuuc     1260

agcucccaua ucucucuuag ccugaaagcg cucgagcaga uucugcccuu uaugcgcgga     1320

gagcgggaag cuagauccag cgacgccgau uacuguaucg gcaucgacga gucuggggaa     1380

ucccaaugcc ugcgcuauga ugagagcgug gaaaaggccg gacuuaaagc ucucucuaag     1440

gacacacuga agggccagau ucugccuccg cucaacgagu ucgagcccua ccuggccaau     1500

ccugucgugg cucgggcccu ugcggaauac agaaaaguuc ugaacgcccu ccugaagcag     1560

uaugggagcc cccacaaaau ccauauugag uacgcucgcg aagccaagcu gaauaccacg     1620

gagcggcaga aauaugaaaa ggagcaaaag gauaacuacg cugccaauca ggcggcccac     1680

aaaaaguguc aggagcuugg acucgaaccu agcuccacaa accugcugaa acucaagcug     1740

uggcaggagc aaggcggccu uugccuguau ucuggggcua aaaucaccau uagcuaccuc     1800

caggacccca cagcccuuca gguggaccac aucuauccgu acuccagauc uuuugaugac     1860

agcuacauga auaagugucu gguccugacc aaggcuaacc aggaaaaagg aaaucggacc     1920

ccuuucgagg ccuuuggcac agauuccgcc aaguggggag ugauuacgac ccucgcucaa     1980

aaacugcccc auaagaaacg cagacggauc cugaacacag cguucaauga caaggaagcc     2040

ggauuuaagc ucagaaaccu gaaugauacc agcuauaucg cucgccuugu ugccgacuac     2100

acagagaccu aucuggaguu ccucucucug guggaauccg agaacacgac acugggcaaa     2160

gggcagaagg gcagcaaaaa gcacauugcc aucgucaacg gagcucuuac cucuaccaug     2220

cgguacuauc ucggcuuugc cuccaaagau agagacaauc aucugcacca ugcgcuggau     2280

gcugugauua ucggguucau gaacgacagc gucauuaagg ccuuuucuga uuucaaaaag     2340

acacaggaaa ccagcaaggc cgcuuacuau gcccacacac ucuccaaaca ggaguacaag     2400

aaacaacgcg cguucaucca gcugccuagc ggagagaauu uuagagcuaa cgugcuugaa     2460

aaggccgagu cuguuuucgu guccaaaccu ccccggaagc gcgucagagg cgcccugcac     2520

gaagagagcu uuuacuccca gaaugacacg aagcuccuga aaucuuaugg gggagcugaa     2580

ggcaucgaga aggcccugca acuugggaaa auucggcaga ucggcaccaa gauugugagc     2640

aacggagcua ugguucgcgu ggauaucuuc aaacauagaa aguccggcaa guuuuacggg     2700

guccccgugu auacaaugga cuucgcccug gggauucucc cgaauaaagc ggugguucag     2760

ggcaaggaca aaaacgaugu caucaaggac uggcuggaga uggauucuag cuacgaauuu     2820

aucuucuccc ucuauaaaga cgaucugcuu cuggugcaga agaaggagau ggccgaaccc     2880

gagcucugcu acuuuguguc uuucgacacc agcggggcuu ccauuaaagu cgccaagcac     2940

gauaauaacu uuagcacacu gaccgaaaau caaaaacugc uuuucucuaa cgcuacgaag     3000

gaggaggugg uuggaaaauc caucggcauu cagaaucuga agguguuuga aaaguggcag     3060

gucagcgugc ucggggaggu uaaacggacc cagucuuauc cucgcgaaaa caucucccug     3120

gccagcaagg ccaaaaagug a                                               3141


<210>  3785
<211>  3144
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3785
augaagcugc ucagauacau ccugggccuu gaccugggga uugccagcgu gggaugggcu       60

aucgucgaga uugaugaaaa cgacuucccc auccggcuca uugauaucgg cguucgcacc      120

uuugacaaag ccgagguguc caagacaggg gaaucucugg cgcuugcuag acggcuggcc      180

cgcagcacga gacggcucau ccgcagacgg gccuuccgcc ugcugaaagg aaagagacuc      240

cugaaacagc acaaugugau uaacaaggag gcucuuacca cacugccuac cgauugcugg      300

gcccuccggg ucaagggccu ggacgaacgc cuuuccccga cagagugguc ugcugugcug      360

cuccaucugc ugaaacacag aggguaucuc agccaacgga agaaugaggc caaauccgcg      420

gauaaggaaa ugggagcccu gcuuucuggc guugcuagca accaucagcu gcucguggag      480

aaucaguuuc gcacccccgc cgaaaucgcu gucaaaaagu ucgcccugga ggagggccac      540

auuagaaacc agcuuggggc guacacacau accuuuaauc ggcuggaccu ccuggccgaa      600

augaagcugc uguuccaaac gcagagagag cugggaaacc cucacaccuc cggccagcuu      660

gaacaggagu uuugugaacu gcucaugugg caaaaacccg cucugagcgg ggaugccauc      720

cuuaagaugc ugggaaaaug cacauucgag accaaugagu auaaggcugc caaacacucu      780

uacacagccg aacgcuuugu guggcucacc aagcugaaca aucugcggau ucucgagcau      840

ggcuccgaaa gaguucugaa cacggacgag cgcagccuuc ugcucaauca gccuuaugaa      900

aaagcuaagc ugacauacac ccaggugcgg aagcugcuug ggcucucuga ggaggcgaga      960

uucaacggag uccgcuaugg caaagaaaag gccgaggaug cuacacugau cgaacugaaa     1020

gccuaccacg ccauucggaa ggcuguggag aaagccgaca aguccgcgug ggaaacccuc     1080

aagaaucaga ccgcucugcu ugaugagauc ggcacaacgu uuagcaucua caaaaccgac     1140

gacgauauua cagagcaacu gaacgggaag cucucugaca ccgugcugaa cgcccugcuu     1200

gaaaaucuca acuucgauaa auuuauccag cugucccuca agagccucua uaauauuguc     1260

ccccugaugg agcagggacu uagauacgac gaagccuguc aacagaucua uggcgaucau     1320

uucgggaaaa aguccggaga gauuaaccgc cugcucccuc cgauccccgc ugacgagauc     1380

cggaauccug uugugcugag aucucugaca caggcccgca aggugauuaa cgcugucauc     1440

cggcuuuacg gcagccccgc ccggauucac aucgaaaccg cgagagagcu cgggaaaucc     1500

uauaaggauc gcaaagaaau ugagaagaga caggaacaaa aucgggccga gagagagaaa     1560

gcuacggccc aguucaagac acuguuuccu accuucaucg gagaacccaa gggcaaagac     1620

auccugauga ugcgccucua cgagcagcag aacggccaau gccuguauag cgggaagccg     1680

cuuaaucuga accggcuccu ggaaaaagga uacguggaga uugaucacuc ucugccuuuu     1740

uccagaaccu gggacgauag ccagaauaac aagguucuug ugcucgcugg cgaaaaucag     1800

aacaaaggga aucagacacc cuucgagugg cugggaaagg acgagcauca augggcccug     1860

uuugucgcgc gcgugaacgg cuguagauuc ccuuacgcca aaaagcagcg gauccucacc     1920

cagaagcugg acgaacaggg guuuauugag cgcaaccuua augauacaag auauaucgcu     1980

cgguuccugg uuaacuuugu gcgcgaaaau cuccaccuga cgggcaaagg aaagaacaga     2040

gucuucgccu cuaauggcca aauuaccgcu uuucugcggg ggcgcugggg acuuagcaaa     2100

gugagagagg aauccgaccg gcaucacgcc cuggaugccg ucgugguugc uugcucuaca     2160

guggcgaugc agcagaagau cacccgcuuc gucaaauacc aagagggcaa ccuccagaca     2220

gccgagagaa uugaccggga aaccggggag aucauccccc ugcauuuucc cgauccuugg     2280

cacuucuuca gacaggaagu gaugauucgc aucuuuagcg agaauccgca ggaggaccuc     2340

auuauccaac ugcccgaucg gccugaagcu aaccacgagu ucguucagcc ccuuuuugug     2400

uccagaaugc cuacgcgcaa gaugagcgga cagggccaua uggaaaccau uaagucugcc     2460

aaacggcugg acgaggggau cuccgucagc aaggugcccc ucacacagcu gaaacugaag     2520

gaucuugaca auauggugaa cagagaacgc gagaaagccc uguaugaggc ucucaaggcc     2580

cggcugaaug cguucaacga ugacccgacc aaggcuuuug ccgaaccuuu cuacaaaaag     2640

ggcggacaac agguuaaagc cgucagagug gaacaggugc agaagucugg cgucgugguu     2700

cgcaauggga acggaguggc ugauaaugcc uccauggucc ggguggacgu uuuuaucaaa     2760

ggcgggaagu auuuccucgu gcccauuuac acauggcaag ucgcgaaggg aauccugccu     2820

gacagagcug ugguugccgc caaagaugag agcgaguggg aacuuaugga caagaccgcu     2880

caguuucugu ucuccauguu ucccaacgau cucauucagg ugaaaacgaa gaaagagaca     2940

uucuuuggcu auuacaacgg gcuggaccgg gccaccggcg cuaucaaucu gaaggagccu     3000

gauucuaaca uuaauaaagg aaagagcggc cuucaccgcu ccaucggggu caagacagcc     3060

cugucugugg aaaaauacca aguggacgag cucggaaaga acaucagacc cugucggccg     3120

agcaaacgcc aggcggucag auga                                            3144


<210>  3786
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3786
augaagagac ugggccucga caucgggacc aacagcauug gauggugccu gaucgaggau       60

gacggcgaug ggaaaggacg gauuaucgac auuggcgccc gcaucuucuc cgaugggaga      120

gaccccaagu cuggagcuag ccuugccgug gaucggcgcg cggcuagagc caugcggcgc      180

agacgggacc gcuaccuggg cagacggaca gccuuucuca aagcucugaa gcgcuucggc      240

cuuaugccug ccgaugaauc cgcugccaaa cugcucgcgg acagagaucc guaugagcug      300

cgggcccgcg cucuggacga aagacucgag ccccacgaaa ucgggcgggc ccuguuucau      360

cuuaaucaga gacgcggauu caagucuaac cggaaggcug agagaaaaac gaaggaugcc      420

gaggacggca aaauugcgaa uggggccaag gcucuggacc aagccauggc ugaaagcgga      480

gccgagaccc ucggccaguu ucuguccucu cgcgauacaa aacgggucag aauggggagc      540

gacgcccagg gauacgauuu cuauccucag cgccggcacg uugaauacga guuugacacc      600

aucuggucca gacaacagca gcaucacccc gcccuucuga cagaggaagc gcgcaagaag      660

cuccaugaua uucuguucuu ucagcggccu cugaaagagc aagaaguggg cguguguacc      720

uucguccaca acgagugcag acuccccaag gcccacccuc uguuucagga acgccggcuu      780

uaugaggagg ugaaucagcu ggaaaucaca accccgggcg cucccaacag aaaacucacg      840

auggacgagc gggaugcccu gauucuugaa cugcgcagca gaaaggagcg gucuuucgac      900

ucccucgcca aaagacugaa gcuggcuccu gggcagagcu uuaauaaagc cucugaaucc      960

cgcacccggc ucagaggaga ugagguuuac gcggugaugg cucauaagaa gcgcuucggc     1020

gacagauggc cccaccugga ucgggaccgc caaugggcca ucauuaaaag aaucaucgag     1080

gaagaggacc cugauaaacu ucaugccuuc cugacagggg aauggggacu caccgacgag     1140

caggcucugg ccacagcucg ggcuccccuu ccggaacugu auggccgccu cgggccuacc     1200

gcgacggccc ggauucugga ggagcugaag aaggauguca ucacauacga cgaagcugug     1260

cggcgcgccg gauggcacca cagcgaugac agaaccggcg agauucuccc ccagcugccu     1320

uacuauggcg aacuucugac acgggauauc ccucccggga cccaagaccc caacgauccg     1380

cccgagcagc gcuggggaaa aauuaccaau ccuacaguuc auaucggccu cagacagcug     1440

gagaagcugg ugaacgcuau cauuaagguc cacgggcggc ccgaccagau cgugguggaa     1500

cuugccagag agcucaaacu gaaugaaaag gauaaagagg aacauaaccg ccggauuaga     1560

cgcgacacgg cggccgcuca acugcggucu gccaagcucc aguccgaggg aaucccugau     1620

accggcgcua auagaaugcu gcuucgcauu ugggaggaac ugaaccccgg ggagguccuc     1680

gaccggagau guccguacug cggcgaaccu guuggaaugc gcaugcuguu ugccggcgag     1740

gccgacaucg aucacaucau ucccuauagc cggucccugg acgauucugc ugggaauaaa     1800

gugguggcgc auagaagcug uaaccgggcc aagggaaacc gcacaccuua cgaaaagugg     1860

guccacgacc ccgauagaug ggacgugauc uccacccagg uugcucggcu ucacaaaagc     1920

aagcaguggc gcuucggccc ugaugccaug gccagagugg agaaagacgg gggauuuauu     1980

gcucggcaac ucacagauac ccaguaucug ucuagacugg ccggccgcua ccucuccagc     2040

cuguauccca cgaaggacga ugggucuguc uacgugaucc cgggccggau gacagcgaug     2100

cuuagacgcc gguggggacu gaauagccuc cugccugacc auaacuucgu ugauaaugag     2160

cacuccaacg cucccaaaaa uagacuggac caucgccacc acgccauuga cgccgcugug     2220

gucgccguga ccacccgggc gcuuaugcag gaaaucgcua gaacagccgg ccgcgccgag     2280

gauaaggacc ucgauagacu guuugaagac cugccccagc cuugggaggg guuccgggag     2340

gaacucggag agaagcuggu ccgcgugacc guuucucaua aaccggauca cggcagaaag     2400

gggcuucccc cuaaaaaccg ggacgugaca gcuggacgcc ugcauaauga uacggccuac     2460

ggccucaccg gggacgucgc ugccgauggc aagacaccca uugugguuca cagagugccu     2520

cugaugagcc ugaaacccgc ggacaucucc gauccugacc ggauccccga cccggcccuu     2580

agaagcgcuc uccaucgcgc caccgaugga cugucuggca aggcuuauga cgccgcccug     2640

gcuggguuug cgaaaacaca cccucaauuc gccggcauuc ggagaguccg cgugcgggaa     2700

ccccucaccg ugaucccuau ugcugauaga gacggacggc ccuuuaaggc cuacaagggg     2760

gaugccaacu cccgcguuga caucuggaga augccggaug gaaaauggcu gagcgacguc     2820

gugacgaugu augaggcuca ccggcgcgau cauucugaca gacggccuca ccccgccgcg     2880

aagaaacuua ugucccugcg ccagaaugau augauugcug uggaaagaga cggcgccccu     2940

cggcagauca uggucgugaa gcagaucugg cccacccaaa uuaaccucgu ugagcauaaa     3000

gagacaggga aucugaagga ucgccacagc aacgaaaccg accccuucag auuuuucgcc     3060

ccuacagcug gcggacugaa gaaaaugaag gccagacagg ugagaaucga cccgcuuggc     3120

cgcauuuuug aucccgggcc ucgguga                                         3147


<210>  3787
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3787
auggccaaca ucgcugagaa ucugguguuc ggcauugacc ucgggaucgg aagcugcggc       60

ugggccgucc ugaaccagaa ggcgaaagau gaaaauauug uugcucuugg guccuggugu      120

uuugacgugc ccgagaccga uaaggaaaga aucccuacaa accaaauucg gcgcgccaau      180

agacugcucc ggcgcgugau cagacggcgc agaaaccgga uggccgagau ccgcagacug      240

uucgcuucua aaggaaugcu ugacacggcc gauccggacg cucuggccaa gauuagcgcg      300

gaucccuggg aaaugcgggc caaaggccuc gacuaccagc ugaccccuca ggaguuugcu      360

gucgcccugg ggcacaucgc uaagcgcaga ggauucaagu ccgccgcgaa acgggugucu      420

acaaaugccc ccagcgagga uuccaagaug cucgcugccc uggaaaagac caaggagcag      480

ucugcucaau aucgcacaau uggcgaaaug uuugccagag acccugccua cgcugaucgg      540

agacgcaacc gggacggcug cuucgauaga accaugagcc gcgaggaccu uuuuaaagag      600

acacagaucc uguucgaauc ccagcggaga cucgggaauu uuagcgcgac cucugagacg      660

gaacaggccu ucauugacau cgcuuuucgc caaaagccca ugcaggauuc cgagcggcug      720

guugaaaccu gucuuuucga gccugacgag aagagagcca gccgcuuugc cccgucuuuc      780

gaacgguuca gacugcucug ccgccuggug aaccugcggg ucacagaugg acucgaggac      840

agaccccuga ccccugaaga acuucggcug gcuugugaag augccggcaa aacacgcaag      900

cucuccguga aaaagguuag aagccugauu gggcuucggg acgaucagag auuuaccacg      960

auucccccug accaggagca acaugauauc gcggcucgca caggagacgc ccugaccggc     1020

acagccaccu uccgggcuau ccucggggag ucuuuuugga gacagcugcu gccccacccg     1080

gaacagcucg aucgcgccgc uuggaucauu uccuuccaug agcugaccga cacaaucauu     1140

agcaaacuug ccacgaucgg acugccuucc gaagugaugg agcucaucag aaaggcgcug     1200

gaagacggca aauuuucuaa guucaagggc gccgcuagcc uuuccgauaa agccgcucgg     1260

aagcugauuc ccuuucucca agaggggcug accuaugaca aagccugcca gaaggcggga     1320

uacaaucacg ccgcuagcaa auucacacau uaugauucua ucgacaccaa ggccaaguuu     1380

aacucccuga uggaugacgu ccgcgauagc auuacaaauc cuguggcuag aaaagccauc     1440

ucugagggca ugaaacaggu gugggccaug cggaaccagu gggggcuccc cggagcuauu     1500

uguaucgaac uggcgcgcga cgucggcaau agcgccgaga aaagacggga aaucgagucc     1560

gggauuaaga aaaacaccgc ucaacgcgag agagaacggc aggaggccaa ggaacaccuu     1620

aaccagucug auaucagcuc cgagacgcug cuccgcuaca gacuguggaa ggaacaggga     1680

ggccggugcu guuauacaga ccaacccauu accccuagcc agcugaucgc cucugauaau     1740

uccguucagg uggaccacau ucuuccgugg agcagauucg gcgaugacuc uuaccucaac     1800

aaagcucugu gcaccgccaa ggcgaaucag gagaaaaaga accgcacacc cugggagugg     1860

auccauaauc aaaaaggggu ggaggcuugg aaccacuuua ccgccgccgu cgagucccgg     1920

aaggaaacaa gaggacugaa gaaacgcaau uuccuccuua agagcuccaa agagacggag     1980

gaacgguuua gaucucgcaa ccugaaugac acccgguacg cugccagagu gauggcggag     2040

gcuguucgcc ugcucuaucc uagcggcaac cgggccgaaa aagggggagu gaagagaguc     2100

uucacacggc ccggcgcccu gaccgcugcc cugcgccaug cuuggggcgu ggagucccuu     2160

aagaaaauua augggaagag acucaacgau gaccggcacc acgcccugga ugcggccguu     2220

guggcugccg ucucugaaaa ggagauccag aaacugacaa agagcuucca gaucugugaa     2280

cagaaaggac ucgcucgccc uaugagagac gugcccaugc cgugggaggg cuuucggcau     2340

caacuggaga ccgccuacgc cgauauuaga gucgcucgcc cugaacggag acgcgcgcgg     2400

gggaagggac acgacgccac gaucagacag cuucgcgagg aaaaagaugg ccccgugguu     2460

uaugagcgga gagcuauuuc cgaccugaag cucaccgauc uggagcgcau caaggacccu     2520

gaaagaaaua gcgccauuau cucuucccug cggacaugga ucgagaaagg gaagcccgcc     2580

gauuacccuc cccagagccc gaaaggagac gaaauucgca agaucagagu gaugucucgg     2640

aacaaaccug cugucuccgu gcgcggcggg accgccgaua gaggcgagau gacacggguu     2700

gacguguuca ccaagauggg aaagaaaggc aagcccgaau gguuucuugu ccccauuuau     2760

agacaucaag ugaucgaccg cauucagugg ccucagccgc ccaaucaggc ggcugccaac     2820

gggaaaagcg agaagaacug gccuacgaug ggacucgagc acgaguuccg guuuucucug     2880

uaccccagaa gcuaugugca aaucguuaaa cgcgauggcg aaaucauuga aggguacuuc     2940

gccggacugg accggacagu cgcugccuuu ucccuccugu cuagcuccga uccuaccagc     3000

auccagagag gcauuggggc gaagacacuu gaguccaucc ggaaguacaa ugugaaccgc     3060

uucggcgaca uuucuauggu ggaacaggag accagaaccu ggcauggaga ugucugcauc     3120

agccccgacc agccugauuc cgcuuga                                         3147


<210>  3788
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3788
augguggaga agauccuggg cauugaccuc gggaucagcu cccugggaug ggccgucguu       60

gaauacgaua aagacaacga ugagaauaac aagauuaucg acugcggcgu gagacuuuuc      120

accgcugccg aaacacccaa agagaaggaa ucuccuaaua aagcgcggcg cgaugcuaga      180

gggcugcggc gcgugauuaa gagacggcgc gucagaauga acacgaucaa gaaucuccug      240

aucaccuaua aacuuauuga caagacacug cucgaugagg agaugggaau guuucacagc      300

caguccaacc ggguggacgu uuggaaacug cgccaugaug cccuguacca ccuccugucu      360

ggcgacgaac uugccagagu gcugauccau auugcuaagc accgggggua uaaauuccuc      420

ggagaugacg agagcgauga agaguccggc aaggucaaga aagccggcgc ugagcugaag      480

aaaaaguuuc uugaagccgg gugucaaucu gugggagagu ggcuguggaa agaacgcggc      540

cuccagggga agaaaagaaa uaagagcgga gacuacgaga ucuccauucc gcgggacuuc      600

cugguugaag agauccagcg cauuuuugag acccagcaaa aguucggcag cacauuugcg      660

accucugaac ugcagaaagc cuauacagau aucgcuuucu acgugaagcc caugcagucc      720

aucgaggaca uggucgggua uugcaccuuu uacccuaaaa uuaagagcaa aaaccaggau      780

ggagaaaaga gagcccccaa ggcuucuccu uccgccgagc aauucgugau ccucagcaaa      840

aucuuuucua cgauugugau ugacgaaaau aagcaggaga aaaagcugau cgagcuuaaa      900

uccaucgaac agcugauuca gaucgcgcgg agcaaggaga cccucaagua caaacaacug      960

agaaaggaac uuaaccuggc caaagauauu uccuucaagu cuaucagcga cgaggaaaag     1020

acauggauua aucucgucgg caacgcuaag uuuaagaaaa uccugggccu gaauuaugag     1080

accuuccuca agaacacaga gaucuccgau gaaauugcca aaauccugac cuacgacaag     1140

acguucgagc agaaagaaac aaaacuuaaa aaucugcucg uuaacauuga uuggaucgac     1200

aacaaucaua uugcugagcu ggccaaacuu agcuuuucuc aguucaacca acugucccuc     1260

aaggccauca aaauuaucag caagaucaug auugaggggu augcucgcua cgaugaagcg     1320

gugcaguaug ccuuugagaa uaaccugcug cccaaaccuu cucacgaaaa gagcauccuc     1380

cugccgcccc uuaaagagac caauauugcu auccugaacc cuacagugau ucgggccuuc     1440

gcccaguuua gacaggucgc uaaugcccuc guguccaagu acggaucuuu cgacaagguu     1500

cacuuugaac uggcgcgcga ggugaacacc aaagaggacc ggaagagaug ggaaaaagau     1560

cgcgacaaga augagaaaau gcaucggcaa aucaccgaaa agcuggucga ggaaggcgug     1620

aagcccagcu auaaaaacau ccuuaagucc aaacucagaa gcgagcagaa ggauacaugu     1680

ccuuacugcc agaaaaaucu gcacuacccc augauuuucg aggacgggua ugcugaaauc     1740

gaucauauuc ugccgcucuc ucagucccaa gacgauagcu acguuaacaa ggugcugguc     1800

cacucugccu guaaucagaa caagaaaaau cgcacgccuu uugagugguu ccaggacgaa     1860

aagaaagauu gggacaccuu uaaguccuau auccuuaugg agagcacacu gggagaaaag     1920

aagcggaacu accucauuaa agagaacuuc uccgaucccc agucuagaaa ggaguuuauc     1980

agccgcaauc ugaacgacac ccgguauaug uccaaggcca ucaaaacaua cugcgaaaau     2040

cacuggaagc ugucucauga ugacgacaaa cuucgcauuc aagugcggag cggcaagcuc     2100

accuccacgc ugcgccacca gugggggcug gauaacaaaa auagagagac acauacccac     2160

caugcuaugg acgccaucau gauugcuuuc agcacccagg gaauggucaa gaagcucucu     2220

gauuauuucg ccaaaaagga agcgaaagug gagaaggaca aaccuguucu gaucacaccc     2280

auuaagcagu uuaaggaggc cguggaacaa gcuaccacac uugagcggca ggaauccauc     2340

cagacgaaag ccggcgauac caucacacug aacagacucc ugauuagcag acccccucgg     2400

gcuucuguca ccggcgccgc gcacgagcaa acagccaagc cguaccccag aaucaaaccu     2460

auuaagaaua aauacaagcg cagacggauc cccauugacg aagauaaguu cgagcuguuu     2520

cgcaacgaca aaguggcuuc cgggaaugau aagaacuucu auaauagcuc uaccaucccu     2580

agaguugaca uuuacaaaaa ggaugacaaa uaucacgugg uccccaucua ccuuagcgau     2640

augacgaagg ccgaggugcc gaacaagucc cucggaacca auccugaagg cauggacgag     2700

aaauauuuuu guuucagcgu guuuaagaac gaccugaucg aacuggagac aaaagcuacc     2760

cccaagaaac cuucuaagaa acuccugggg uacuucaagc agcuuaaugg agccaacuuu     2820

auucugaacu ccauccauaa uggcauuauc gauggguucg uuugcagccc cauuacacuc     2880

uuuaagcagc agaaagacau guguaagaaa ugccugccug aagaucgggc caucggcaac     2940

uguucccaag agacccugga guucugggaa gcugagaaua ucaagguccc caaaaaggac     3000

uuugaaugcg aucagggaau uaaguucgcg aucgccgugc gcaaauauac gauugacccg     3060

cuuggcuacu accacgaggu gaagggggaa aaacuccugg gaacaauccc ucagggcgcu     3120

aagaaacauc ccaagagaca gaaguga                                         3147


<210>  3789
<211>  3150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3789
augaucaacg agagacugac cuucggcauu gaccucggga ucggaagcug cggcugggcc       60

gugcugcggc acgcugaugc cgcgggggaa cccggaguca uugagggccu uggguccugg      120

uguuuugacg uuccugaaac aucuaaggag cgcaucccga cgaaucaggu gagacggagc      180

aaccgccugc ucagacgggu gauucgcaga cggcgcaaua gaauggcuga aauccggcgc      240

cuguuccaug cccaaggacu ucugcccucc gccgaugagg acgcucucaa aagaccucgg      300

cuggaucccu gggagcugcg cgccagaggc cucgaccagu gccugucugc ugaagaguuu      360

gccgucgcgc uuggccacau cgcccggcau agaggguucc gcagcuccgc uaagcggaaa      420

gccgcuaaca ccucuggaga agauagcaag augcuguccg cccuccuggu gacaagagag      480

cgcaccgcgc gguacagaac aguuggcgag auguuugccc gcgacccuga auauagcgcu      540

cggagacgca aucgggaugg gauuuucgac agaaccgugg cccgcgauga ccuuauccac      600

gaggucggaa aacuguuuga agcucagcgg gccagaggcu cugccuucgc uucccccgau      660

cucgaggcgg acuuuaacgc cauugcuuuc cggcaggccg aaaugcaaga cagcgagcgc      720

cuggugggga auuguccuuu ugagagagau gaaaagcggg ccgcuagaug ggccccgucu      780

uucgagcgcu uucggcugcu cacaagacug aucaaccuuc gcauugcgac cgcugaagga      840

gagagacggu ucacgcccga agaccuggcc cgcguuuccg ccgagcucgg cgcuaccgcc      900

aagcugccug ugagcagagu ccgggcucuu aucggccugc uccccgauga gcgcuucaca      960

accauuagag uggaagacga ggaucgggac aucguggccc gcacagggga agcggccccu     1020

ggaaccugga gacugcggaa aacgcugggc gaggcucucu gggccgaaau gcaggcucgc     1080

cccgaucagc uggacgccau cgcgcauguc cuuagcuuuu ucgaggccac agcuaagauu     1140

agauccgagc uggaucggcu cgggcugccg gccgcuguua ucgaagcccu ugacagagga     1200

cuggaggaug gcgccuuugc uaaauucaag ggggcgggac acauuagcgc caaagcgugc     1260

cgcaaucucc ugccucaucu gcagcaaggc caccgguacg accaggccug uagacucgcc     1320

ggcuaugacc acgcugccuc cacccugucu cauacagcuc agguggugag caaggcuggg     1380

uuuaacgccc uugucaccga acugggagag uccaucgcca aucccauugc ucgcaaggcc     1440

cucaccgagg gccugaaaca gauccgggcg augugcgcua gaugggggcu gccuggagcc     1500

auccacauug aacuugcccg cgaggugggc aacagcaucg aaaagcggag agagcucgaa     1560

ucucaacgcg agacaacgac caaacggaga cagacagagc gggaugaaau ucgcgggcug     1620

cuggcucucg aggacguuga ugacgauacc cugcuuagau accggcugug gaaggaacag     1680

ggcggaaaau gucucuauac aggcaaggac auccacgugc gccaacugau ugccaccgau     1740

aauuccgucc agguggacca uauccugccc uggagcagau ucggggauga cucuuuuaac     1800

aauaaaacgc uuugccucgc uagcgccaac cagcggaaga gaggacagac acccugggag     1860

ugguucuccc ugaccggcac cccugaagcg ugggagguuu uugccucucg cguggagaca     1920

agcaaggauc ugcggggguu caaaaagaga aaccuccugc uuaaaaaugc ugacgaaguc     1980

gcccaacgcu uucgguccag aaaccugaau gauacccgcu acgcugcccg gcuccuggcc     2040

gaggcugcga agcuguucua uccggccgga gacagacagg aaaaagacgg ccgcagacgg     2100

gugcuuacac gccccgggcc ucucacggcu gcccugagac acgccugggg ccuggagagc     2160

cucaagaagg cugauggaaa acgggucucu gacgaucgcc aucacgcccu ggacgcgcuu     2220

gcuguggccg ccaucuccga aagagagguu cagcggcuga ccagaucuuu ucaggagugg     2280

gaacaacagg gccucgcucg cccccugcgg cauguggccc cucccuggcc gggguucaga     2340

ggagaugucc uggcggcuua cgcccaggcc uucguggcuc gcccugagcg gagacgggcc     2400

cgcggcaagg ggcacgacgc uucuauuaga cagguugugg gagaaggccc cgaggcccaa     2460

gucuuugagc ggcgcgggcu ugcggaucuc aaagaagccg accuggauag agugaaggac     2520

ccugagcgga accaggcuau cauugaagcc gugcgcgcuu ggauccuggc cggcagaccc     2580

gccgaugcuc cuccccgguc cccgcgcgga gacguuauug ucaaacucag aacagcgccu     2640

gccaagaaga ucaaacccgc ugugcccgug cggggcggga ccgccgauag aggagacaug     2700

guccgcauug acguguucac acggccuaau agaaagggcc gcgaugagug guaccugguu     2760

ccgaucuauc cccaccaggu gaugaaccgg aaagccuggc cucauccccc uaugagaagc     2820

gucguggcuu acaaggacga agccgggugg accgagaucg gagcggagca cuccuuucgc     2880

uucucucuuu auccccggag cuacguucag cuggugaaau ccaauggcga ugucauugaa     2940

ggguaucucg ugucuaugga cagaagcacg ggcgcuuuuc gcguuuucgu gcauaacaau     3000

ccugccgaga uggccaaggg aaucggcgcu aagacccugc ugaaccuucg gaaauuuaau     3060

gucgauagau ucggggugcg cacagaagug aaguccgagg uccggaccug gcacggagaa     3120

guuuguacaa gccccgugcc gccugacuga                                      3150


<210>  3790
<211>  3150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3790
augcagcuga gcaagcucaa cuacauccug ggccuugacc uggggauugc cuccguggga       60

uggucuguca ucgagauuga ugaaaccgag augcccauca gacucauuga cguuggcgug      120

cggacauucg aacgcgcuga ggugccuaaa acgggggaaa gccuggcccu ugcgagacgg      180

cuggcucgcu ccaccagacg gcucauccgc agacgggccu uucgccugcu gaaggccaaa      240

agacuccuga agaaucacca aaucgucucu gcugaugagc uuagcacacu gccgaaccau      300

ugcugggagc uccgggugaa aggacuggac gaacgccuua ccugucagga gugggccgcu      360

guucugcucc accuucugaa gcauagaggc uaucucuccc agcggaaguc ugaagcccag      420

aaugcggaua aagagauggg ggcccugcuu agcggagugg cuaacaauca caagcugcuc      480

uccgagaacc auuaccgcac acccgccgaa auugcuguca aaaaguucgc ccuggaggaa      540

ggccacauga gaaaucaacg gggcgcguau acccacacau uuaacagacu ugaccugcuc      600

gccgagaugc gccugcuguu ccagacccag cgggaacucg ggagcccuua cgcuucugga      660

gagcuggagc aggaauuuug caaacuucug auguggcaaa agcccgcccu cuccggcgau      720

gcuauccuga agaugcuugg gaaauguacg cuggagccua ccgaauauaa ggccgccaaa      780

aauacauaca ccgcugagag auucgugugg cucacaaagc ugaacaaucu gcgcauucuc      840

gaaaacggaa gcgagcgggc gcugaaugcc gacgagagag cucugcugcu caaccagccc      900

uaugaaaaag ccaaguuuac cuacgagcag gcccgcaaac ugcugcagcu uaaugaagcu      960

gcccgguuca acucuguuag auacggcaag gagaacgcgg aaaaguccac gcucauggag     1020

augaaggcuu aucaugccau ccgcaaagcc guggagacag cugauagcuc ugccugggcu     1080

acccugaaaa cacaaccucu gcuccuggac gaaauuggga ccgccuuuuc caucuacaag     1140

accgaugagg acauuaaaac acagcuucag ggaaagcugg cggauaaugu caucgacgug     1200

cuccuggaaa accugaauuu cgacaaguuu auccaacuua gccucaaagc ccuguauaag     1260

auucugccgc ucauggagca gggccugcgg uacgaugagg cuugcuccca gaucuauggc     1320

gaccacuucg ggaaaaagga acagaaaaac gauagacuuc ugccccaaau uccugccgac     1380

gagauccgca aucccguggu ccuccggacg cugucucagg cuagaaaggu uauuaacgcc     1440

gugauccggc uguacggaag cccugcgcgc auccauauug aaaccgccag agagcuuggc     1500

aaguccuaua aagaucggaa ggaaaucgag aaaagacagg agcagaaucg cgcugaacgg     1560

gagaaggccg uggacaaauu uaaggaacuc uuccccaacu ucauugggga gccgaaggcu     1620

aaagauaucc ugacaaugag acuguacgaa caacagaaug gacagugucu cuacagcggc     1680

aagucucugg accuucaccg ccugcucgag aaaggguaug ucgagauuga ucacgcccug     1740

ccuuuuucca gaaccuggga cgauagcuuc aacaauaagg ugcugguucu ugccggagaa     1800

aaccagaaua aaucuaacca aacacccuuu gaguggcucg gcaaggacga acagaaaugg     1860

gcucuguuca ccgcgcgggu gaauagcugc cgcuuuccuu acuccaagaa gcagagaauc     1920

cugacgaaaa agcucgacga ugagggcuuc auccagcgga accugaacga cacacgcuau     1980

auugccagau uucuugucaa uuucgugaac gauaaucugc aucucaccgg gaaaggaaag     2040

cggcgcguuu uugcuucuaa cggccaaauc accgccuucc ugagagggcg guggggacug     2100

acaaaagugc gcgaagagag cgacagacac caugcccuug augcugucgu ggucgccugu     2160

uccaccguga gcauucagca gaagaucaca cgguuuguua aguaccaaga gggcaaucug     2220

cagacggcgg aaagaauuga ccgcgagacc ggggaaauca uuccccucca cuuccccgau     2280

ccuugggccu uuuuccggga ggaggugaug aucagaaucu ucucugaaau gccgcaggag     2340

gaccugguca uuaaacuccc cgaucgcccu caggcuaacc augaauuugu gcaaccccug     2400

uucguuuccc ggaugccuac aagaaagaug agcggccagg gacacaugga gaccaucaaa     2460

ucugccaagc gccuugacga aggcauuucc gugagcaaag ucccccugac acagcucaag     2520

cugaaggauc uggagaauau ggugaaccgg gacagagaga ucgcucuuua ugaagcccug     2580

aaagcgcgcc ucaccgaguu uaaggaugac ccggcuaaag ccuucgccga accuuuuuac     2640

aagaaagggg gagugcaggu uaaguccguc cggguggagc aagugcagaa gucuggcguc     2700

gugguuagaa augggaacgg aguggcugac aaugccagca ugguccgggu ggauguuuuc     2760

auuaaaggcg ggaaguauuu ucuggugccc aucuacacgu ggcaggucgc uaaaggcauc     2820

cucccugacc gcgccgugau ugcguacacc cccgaaaacg agugggagcu gauggaugac     2880

uccgcccagu uccaauuuuc uaucuauccu aaugaucuua uuaagcugac aaccaaaaag     2940

gcuaucuucu uuggauacua uagcggcuuc aacagacuca caggggccau uaauaucaaa     3000

gaacacgacc ugcagaucuc caagggagau aagggcguuc aucuggggcu uggagugaaa     3060

cuggcucuca gccuggagaa guaccagguc gacgaacucg gcaaaaacau ucggcccugc     3120

cgcccgaccc aaagacagaa cgugcgguga                                      3150


<210>  3791
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3791
augagcuccu acagauucuc uuuugaccug ggcaccaaca gcaucgggug ggccguguuc       60

gagcucgauc ccaagacacg gccugucgcu cuggccgacu gcggaguucg cauuuuuccg      120

gauggcagaa aucccaaaga caagcagucc cuugcggugg cucggcgcga agccagagcc      180

gugcggcgca gacgggaucg cuaucugaaa agacggucug agcucaugga acgccugguc      240

gcuaaggggc uuaugccuga gagcagagac gaucaaaaag cccuggaagg acucgacccc      300

uacgcucugc gggccgcggg ccuggaugac gcccucccuc cccaccauuu cgggcgcgcu      360

cuguuucacc uuaaccagag acggggauuc aaguccaaua gaaagacgga ucgcggcgag      420

aacucugaga ccggcaucac aaaaauuggg augaagaaac ugcaugaagc ccuccugagc      480

gagggauguc ggacccuugg cgaacugcag cacagacauc acgaggcuaa ucagaccccu      540

gccauggaca ccaacccgaa guccgugcgc agcaaacucc ggggagaggg agcgaaggcc      600

guuuaugauu uuuaccccuc uagagacgcu guggaagacg aguucaauaa gcugugggau      660

ucccaaaaac gcuuucaccc ugaccugcuc acagauagcg cccgggacgc uaucagagau      720

gccauuuucu aucagcgcaa gcugaaaacc cccgacgccg gccggugcuc uuuuaacccu      780

gcugaagaga gagucgcgcg cgccaugccc aucgcucagc auuuccggau ccuucaguuu      840

gugggggauc ugagacucau ugacaaugau cugguucggc acccgcuuaa caaugaccaa      900

aagaaagccc uguaccgcga ucuccuggcc aagggaaaac ugacguucaa ggcuaugaga      960

aagaccaucg gcauuggcuc cgaauaugcc uucagccaug agucugagaa acaguccgaa     1020

uuuagagggg acgagacagc ggcucggcuc gccggaccua aguacuucgg ccccaaaugg     1080

cgcgaacuga gcuuuaacga ccaguccgag aucguggauc ggcuucugca cgaagagaau     1140

gaggaaaccc ucauucagua ccugcaagac agauucgggu uugaucacga ccgcugugag     1200

gucaucgccg augcuccucu ucccgacgga cauacaagac ugggcaagac cgcccucgaa     1260

acgcuggugg agauuaugcg ggaugaccac cuucccuaug aucaggcuug cacaaaagcc     1320

ggguacgaac augcgcuccc uccggacgga gugauccugg augaccuucc cuauuacggc     1380

aucgcccugg agcgccacgu cgcuuucggc gaugggaagu cugagaaccc ugaaaagaga     1440

uauggaaaaa uugccaaucc caccguucau aucgcucuca accagcugcg gaaggugauu     1500

aacgcccuga ucgcgcgcua cggccacccu gaccaagugg ucauggagau ugccagagac     1560

cuuaaacaga gcaaggcuaa acgggaugac aucuccaagg aacagaagaa aaaucaggau     1620

cgcaacgacc aaagacggaa gaaacuccag gaggcccaca ucgcugugaa ugccgagaac     1680

augcugcgca ugagacugug ggaggaacuc gaugaagaca augccgcugc gcgggccugu     1740

cccuuuacag gggagaccau uagcauggcu agacuguucu cuccggaagu ugagaucgag     1800

cauauucuuc cuuuuucccg cagccuggau aacucucccg ccaauaagac ccucugcaug     1860

gugugggcca accgggcuaa aggaaauaga acaccuuggg aagccuucgg ccgcgaccgg     1920

gagcccgggu uugauuggcc ugaaaucgcg gcuagagccg cccgccugcc caagaacaag     1980

agcuggcggu ucggcccgga cgcuauggag agacuggaac gcgagggaga uuuucuugac     2040

cggcagcuca cggauaccca guaucugucc agaauugccc gggaguacgu cacaaaaauc     2100

ugugacccua aucaagugug gguuaccccc ggccgccuga cagcguggcu cagacggaac     2160

ugggggcuga auggaauccu ucccaccggc aacgcuacga agaaucgcga cgaucacaga     2220

caucacgcca uugacgccau cguggucggg cugacagauc gggggauucu caacagagug     2280

ucucgcagcg cugcccuggc ugaagccgag gaccuggauc gguccaucgc ggaaaugccu     2340

gacccguucg auggcuucag agacaacauu cgccacaucc uugagcagau uaucgucagc     2400

cauaaacccg augccggguu ugugccuggc caccccaagg gaaauaccac cccuggcgcu     2460

cuccauaacg acacagccua cgggaucauu aaaaccgaug cuaagggaau gucugaaguu     2520

gugacacgga aagacguccu guccuucaag ggcacgaagg agaucgagag cauuagagac     2580

gccgccaccc gccagucucu gcucgaugcu guggcggggg uucccuccgg acuggccuuu     2640

aaagaagcuc ugcgggacug gagcgugaga acaggcacca agcgcgucag agugacauuc     2700

aauauguccg ugaucccuau ucaggaucgg gacgggaaag ccuauaaggg cuacaaagga     2760

gauggcaacc ugugcgccga gaucuaugaa cgccccgacg ggaagugggg agaugagauc     2820

auuucuagau uugacgcugu ucaaccgggc uucaccccuc aguggcagcg ggaucacccc     2880

gccgcgcauc gcgucaugag acucuuucgg gacgauauga ucauggcuga gauugacggg     2940

gugaugacgg uguguagaau cgucaagaug agcccuggaa aagugguuuu cacaccccac     3000

cacgaagcca auacagauuc ccgcaaccgg gacaaggacg auccguuuuc uuuccauacc     3060

ugcagcggcg agucccugag aaaacgcaag gcccagaaac uguuugugga cccucuuggg     3120

aagauucggg gcuacagagc uaaggaagga uga                                  3153


<210>  3792
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3792
augggcgaga acaugaucga cgaaagccug accuucggga uugaucucgg aaucggcucc       60

ugcggguggg ccgugcugag acggcccucu gcuuuuggac gcaagggcgu cauugagggg      120

augggaagcu gguguuucga cguuccugaa acauccaaag agagaacgcc gaccaaucag      180

auccggcgcu cuaacagacu ucugcggcgc gugauuagac ggcgcagaaa ucggauggcc      240

gcgauccgca gacuccugca cgcugccggc cuucugccca gcacagauuc cgacgcccuc      300

aagcggccug gccaugaucc cugggaacug cgcgcuagag ggcuggacaa accucucaag      360

cccguggagu uugccgucgu gcugggacac aucgcuaaac ggagaggcuu caagucugcc      420

gcgaagcgca aagccaccaa cauuagcucc gaugacaaga aaaugcuuac agcucuggag      480

gccacccggg aaagacucgg gcgcuaccgg acaguuggag agauguuugc uagagauccu      540

gacuucgcca gccgccggag aaaucgcgaa ggcaaguaug aucggaccac ggcgagagac      600

gaucuggagc augaggugca cgcccuuuuu gcugcccaac gccggcuggg gcagggauuc      660

gcuucuccgg aacucgagga agccuuuacc gccuccgcuu uccaucagag acccaugcag      720

gacagcgagc ggcuggucgg cuuuugcccu uucgaacgca cagagaaaag agcggccaag      780

cugacccccu cuuuugagcg guucagacuc cuggcucgcc uucugaaccu ccggaucaca      840

accccugacg gcgaaagacc ccugacggug gaugagauug cccuuguuac acgcgaccug      900

gggaagaccg ccaaacucuc caucaagaga gugcggacac ugauuggacu ggaagauaau      960

caacgcuuca ccaccaucag accggaggac gaagaucggg acauugucgc ucgcacaggc     1020

ggggccauga cgggaaccgc gacacucaga aaagcucugg gcgaggcccu uuggaccgau     1080

augcaggagc ggccugaaca gcuggacgcc aucguccagg ugcucagcuu uuucgaggcu     1140

aacgaaacaa ucaccgagaa gcugcgcgaa auugggcuua cgcuggccgu gcucgauguu     1200

cugcugacag cucucgacgc cggaguguuu gcgaaauuca agggcgccgc ucacaucucu     1260

accaaagccg cuagaaaucu gcuuccccac cuggagcaag gccggcgcua cgaugaggcc     1320

uguaccaugg cgggguauga ccaugccgcu uccagacuca gccaccaugg acagauugug     1380

gccaagacac aguuuaacgc ucuggucacc gaaaucggcg aguccauugc caauccuauc     1440

gcccggaagg cucugaucga agggcuuaaa cagauuuggg cgaugagaaa ccacugggga     1500

cuccccggcu cuauccacgu ggagcuggcc cgcgacguug ggaauagcau ugagaagcgg     1560

agagaaaucg agaaacauau ugaaaagaac acagcucugc gcgcccggga gagacgcgaa     1620

gugcacgauc uccuggaccu ugaggauguc aauggcgaca cgcugcuccg guacagacug     1680

uggaaagagc aaggaggcaa gugccuguau accgggaagg ccauccauau ccgccagauu     1740

gcugccacag auaacuccgu gcagguugac cacauccuuc ccuggagccg guucggagau     1800

gacucuuuua acaauaaaac ccucugucug gcguccgcua accaacagaa gaaaagaagc     1860

acaccuuacg aauggcuguc uggccagacc ggggaugccu ggaaugccuu cgugcagcgg     1920

auugagacga acaaggaacu ccgcggauuu aaaaagagaa auuaucugcu uaagaacgcu     1980

aaagaggccg aagagaaguu ccggagccgc aaucugaacg acaccagaua cgcggcucgg     2040

cucuuugccg aggccgucaa acugcuguac gcuuucggcg aaagacaaga gaaagggggc     2100

aaucgccggg uguuuacaag accgggagcc cuuaccgcug cccuccgcca ggcguggggc     2160

gucgaauccc ugaagaagca ggaugggaag cggaucaacg acgauagaca ucacgcccug     2220

gacgcucuca caguggccgc uguugacgag gccgaaauuc agcgccugac caaaucuuuc     2280

cacgaguggg agcaacaggg acuuggccgg ccccugagac gcguggaacc ucccugggag     2340

agcuucagag ccgaugucga agcuacguau ccugaggugu uuguugcgcg gcccgagcgc     2400

agacgggccc gcggggaagg acaugcugcc acaaucagac aggugaagga gcgggaaugc     2460

accccgauug ucuucgagag aaaagccgug uccagccuca aggaagcuga ccuggagcgc     2520

aucaaagaug gcgagcggaa ugaagccauc guggaggcga uuagaucuug gaucgcuaca     2580

gggcgcccug ccgacgcucc cccucggucc cccagaggcg auauuaucac caagauucgg     2640

cuggccacca caaucaaagc cgcgguuccu guccgcggag gcacggcugg gagaggagaa     2700

auggugcggg ccgacguguu uagcaagccc aaccgcagag gcaaggauga gugguaccuu     2760

gucccggugu auccucacca gaucaugaau cggaaagccu ggcccaagcc cccuaugcgc     2820

ucuauuguug cuaacaaaga cgaagaugag uggaccgagg uggggccgga acaucaauuc     2880

agauuuuccc ucuacccccg gagcaauauc gagauuaucc gcccuuccgg agaagucauu     2940

gagggcuauu ucguggggcu gcacagaaac acaggcgccc ugaccaucuc ugcucacaac     3000

gaccccaaga gcauccauuc cggaauuggc acaaaaaccc uccuggccau cucuaaguac     3060

cagguugauc gguuugggag aaagagcccu gugcgcaaag aaguccggac guggcacgga     3120

gaggcgugua uuucccccac accucccggc uga                                  3153


<210>  3793
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3793
augaagaucc ugggcuucga cauugggauc gccagcauug gaugggcuuu uguggagaac       60

ggcgaacuca aagauugcgg ggucagaauc uucaccaagg ccgagaaucc caaaacagga      120

gacucccugg cgaugccucg gcgcgaagcu agaucuguuc ggcgcagacu ugcccggcgc      180

aagggcagac uggagacgcu caaacggcug cuugccaagg aaugggaucu guguuacgag      240

gacuauauug cugccgaugg ggagcucccg aaggcuuuua ugggaaagaa ccugaauaac      300

cccuacgugc ugcgcuauga agcccuccag agacugcuua gcaaggagga acuggugcgg      360

gucgugcucc acaucgcgaa acaucgcggc uacggcaaua agaacgccaa aaucaccaag      420

uccgaggagu cuaagagaga acaagggaaa auucugagcg cucuugccac aaaugcuucc      480

gccaucgcgc gguaucgcac cguuggagag uacuucuaua aggaauuuug cgaggugauu      540

aaaaacccuc agggccuguc uacaaaugaa cacuacaccc agagcaaggu cagagugcuc      600

aaacccaucc ggaacaaagg gggagaguac acaaauugua uucugcagga ggaccugcaa      660

agagaacucc gcugcaucuu cgaguaucag aaaggcuuug gguucccuau uacccaggaa      720

uuucaggaua agauccugaa gaucgccuuc uaccaacggc cccuuaaaga cuuuucccau      780

cugguuggaa aguguacguu cuauccugau gaguuuagag cucucaaaug cagccugucu      840

gccguggagu ucauuacccu uuccaagaca aucaacgucc ugaccuacau uagcaaagaa      900

ucuggcgagg uguauuccaa gggccaguac gaacagaugc ucgacagcgu guucaaggcu      960

gucugugaga aagggucucu guccuuugcc cagcugcgca agauccucca cauugaugaa     1020

cggaucagau ucaaagaggu ugacuauaug gcccgcaaga gcaaaaauac auccggagau     1080

ucuacccaua agcaaaagac gagcaacccg gaaacgaaaa aguuuaucga gcacaaaaau     1140

cugaagaaau ucugcgagau ucuuuccgaa aucgcugagg accgggcgcu ggccgacagc     1200

auugcuagag auaugacccu caucaaggac gaagagaagc uggcccagaa acuucacggc     1260

uacgggaagu uuucucagga gcaaauuaca cagcuguccg cccucaacuu cagccaucac     1320

aucaaccugu cucugaaagc ucucagcgaa auccugcccu uuaugcgcga gggaaugcgg     1380

uacgaugaag ccuguaaacu ugcgggccug aaagcuagac auaaugacaa gaaauccaag     1440

uuccucccuc ccuuuugcga gucuauuuau gccgaugaac ugaccaaccc uguggugcac     1500

cgcgccaucg cugaguaccg gaagauucug aaugcccuua ucgcuaaaua ugggagcguc     1560

cacaagauuc auaucgagcu caccagagac gugggaaaga acuuccagga acgggagaag     1620

uacaaaaagg aaauugaguc caauuauaag gcccgcguuc aggcgaugca agaaugugag     1680

aaacugggcc ugacacucag cgagggcaac auccugaaac uuagacuguu ucgggaacag     1740

aaugagaucu gcguguacuc ugggagaaag auuacgcucg ccaaccugaa ggaacaggga     1800

gcucuggaga ucgaucacau ucuucccuau ucccgcagcu cugacgauuc cuacaugaau     1860

aaaguccucg uguucaccaa cgaaaaucag aacaagggca auaagacacc guacgaggcc     1920

uuugggggag acagccaaaa auggggcgag aucgaauccc uggcucugcg gucuggguau     1980

ccuaagaaaa aggccaaacg cauucucgau aagggcuucg gagacagaga ggcgggcuuc     2040

aagagcagaa acaucguuga cuccggguac aucgcccggc ugauugcuaa uuauaccaaa     2100

gaauaccuua aguuucugcc ccucgauucu caugagaaca cagcccugau cgcuggagag     2160

aaagacagca agauucacgu ggaagccguc aaaggcaugc ugaccgccac gaugcgccau     2220

uucugggggc uuggauccaa gaacagauau gagcacacac accaugcugu ggaugcgauc     2280

auuaucgccu acaucaaugc ugccaugauu cagcgguuua gccaguuccg ccagaaccaa     2340

gaaucucuca aagccagauu uuaugcuaag gagcuggcca aggaagaguu caaaacccag     2400

cgggcguuuu ucgagccuuu ucgcggcuuc agagaacagg uccuggcuaa gguggggcag     2460

aucuuuguuu cccggccucc ccgcaaaaga gcccggggcg cccuccacga ggaaaccuuc     2520

uacagcauug acgauaagaa acugucugag acauacggag gcaaggaagg ggugcaaaga     2580

gcucuuuccc ugggaaagau ccgccagauu ggcaccaaaa ucgucgccaa ugggcccaug     2640

gugcggguug acaucuuuaa gcauaaacag agcagacgcu ucuaugcggu gccgauuuac     2700

acaauggauu ucgcucucgg aauccugccc aacaaggccg ucgugucugg caaagacaca     2760

aagagcaagg ugauuaaaga uuggcuggag auggacuccu gcuaugaguu uuguuucagc     2820

cuuuuuaagg augaccucau cgaaguucaa aaaucugaua uggagaagcc ugaacuggcc     2880

uacuucgucu cccuggacgc uagcgacggg cggauuaaag ugagacacca cugcaaugau     2940

aucaccaaga uuaacgagaa ucagaagaaa cucuuuuccg aagccaucga gaaggaggug     3000

gucggccgcg gaucuaucca gaaccugaaa guguucaaga aauauaaggu uagcccccuu     3060

ggcgaaauua aggaggcuaa auacgaaccu cggcagccca ucgcccugaa gacauccccg     3120

aaaaagcaua gaaaacaaaa uaacgcgcag uga                                  3153


<210>  3794
<211>  3156
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3794
augaacaauu acauccuggg ccucgacauu gggaucacca gcgugggaua uggcauuguc       60

gauuccgaca caagagagau caaggaugcc gggguucggc uguuccccga agcuaacgug      120

gacaauaacg agggacgcag aucuaaacgg ggcgcccgca gacuuaagcg gcgccggauu      180

cacagacugg aucgcgugaa acaucuccug gcggaauacg accuucugga ucucacgaau      240

aucccuaaga gcaccaaccc guaucagaca agagucaaag ggcugaauga gaagcugucc      300

aaggacgaac ucgugaucgc ucugcuucac auugccaaac ggcgcggaau ccauaacguu      360

aaugugauga uggaugacaa cgauucuggc aaugagcuga gcaccaagga ccaacucaaa      420

aagaacgcca aagcucuguc cgauaaguac gucugcgagc uucagcugga aagauuugag      480

caggacuaua aggugcgggg cgagaaaaau cgcuucaaga cagaagacuu uguuagagag      540

gcccggaaac uccuggaaac ccagucuaag uucuuugaga uugaucaaac auucaucaug      600

agauacauug aacugaucga gacccgccgg gaauauuuug aggggcccgg aaaaggcagc      660

ccuuucgggu gggagggaaa cauuaagaaa ugguuugaac agaugauggg ccacuguacg      720

uacuuccccg aggaacucag auccgugaag uacagcuauu cugcugagcu guuuaaugcc      780

cuuaacgacc ugaacaaucu cgucaucacc cgcgaugaag acgcgaagcu gaacuacggg      840

gagaaauucc agaucauuga gaauguguuu aagcagaaaa agacaccuaa ccuuaaacaa      900

aucgccauug aaaucggagu gcaugagacc gaaauuaagg gcuaucgggu caauaagucc      960

ggcaaacccg aguucacaca guucaagcug uaccacgauc ucaaaaacau cuuuaaggac     1020

ccuaaauauc ugaaugauau ccagcugaug gacaacauug cugaaaucau uaccaucuac     1080

caggaugccg agagcauuau caaggagcuc aaucaacugc cggaacuucu gucugagaga     1140

gaaaaggaga aaaucuccgc ucucagcggg uauucuggaa cgcaccgccu gucccuuaag     1200

ugcauuaacc ugcuccugga cgaucugugg gagagcuccc ucaaucagau ggaacuguuc     1260

acaaaacuua accugaagcc caaaaagauc gaccucucuc agcaacauaa gauuccuagc     1320

aaacugguug acgauuuuau ccuguccccc guggugaagc gggccuucau ucagagcauc     1380

caggucguga augcgauuau cgacaaauac ggccuuccug aggauaucau uaucgaacuc     1440

gccagagaga acaauucuga cgaucgccgg aaguuucuga accagcugca aaaacagaac     1500

gaagagacca gaaagcaggu ugagaaagug cuccgcgaau acgggaauga caacgcuaag     1560

cggauugucc agaagaucaa acugcacaau augcaagagg gaaagugucu uuauucccug     1620

aaagauauuc cccucgaaga ccugcugaga aacccgcauc acuacgaggu ggaucacauc     1680

aucccucgga gcguugccuu cgacaauucu augcauaaca aggugcuugu ccgcgcugau     1740

gaaaauagca aaaagggcaa cagaacaccc uaucaguacc ucaauuccuc ugagagcucc     1800

cugagcuaua acgaguuuaa gcagcacauu cugaaucucu cuaaaaccaa ggaccggauc     1860

accaaaaaga aaagagaaua ccugcuugag gaacgcgaua uuaacaaguu cgacgugcag     1920

aaggaguuua ucaaucggaa ccuggucgac acaagauaug ccacgcgcga acucaccucc     1980

cugcugaaag ccuacuucag cgcuaauaac cuugauguga agguuaaaac aauuaacggg     2040

ucuuuuacca auuaccucag aaaggugugg aaauucgaca aggaucggaa caagggauau     2100

aaacaucacg cggaggacgc ccugaucauc gcuaaugccg auuuucuguu caagcauaac     2160

aaaaagcucc gcaauauuaa caaaguccug gacgccccuu ccaaggaggu ggauaaaaag     2220

agaguuacag ugcaaagcga agacgaguac aaucagaucu ucgaagauac ccagaaggcu     2280

caggccauua aaaaguuuga gauccggaaa uucucccacc gcgucgacaa gaaacccaac     2340

agacaacuua uuaaugauac gcuguauucu acacggaaca ucgacggcau cgaguacgug     2400

guggaaagca uuaaggauau cuauuccguu aauaacgaca aggucaaaac caaguuuaaa     2460

aaggaccccc accgccuccu gauguacaga aaugauccuc agaccuucga gaaauuugaa     2520

aagguguuca agcaguauga gucugagaaa aacccguuug cgaaguacua cgaagagaca     2580

ggcgagaaaa uucggaaguu cagcaaaacc gggcaaggac ccuauaucaa uaagauuaag     2640

uaccugcgcg aaagacuugg ccggcauugc gacgugacaa acaaauauau caacuccaga     2700

aauaagauug uccagcucaa aaucuacagc uaucgcuuug auaucuacca guaugggaac     2760

aauuacaaga ugauuacgau cucuuacauu gaccuggaac agaaauccaa cuauuacuau     2820

aucagccggg agaaguacga acaaaagaaa aaggauaaac agauugacga uucuuauaag     2880

uucaucggau ccuuuuacaa aaaugacauc auuaacuaua auggcgagau guacagagug     2940

aucgggguua acgauagcga gaagaauaaa auucagcugg acaugaucga uauuucuauc     3000

aaggacuaca uggaacucaa caauaucaag aaaaccggcg ugauuuauaa gacaaucgga     3060

aaaagcacca cacacauuga gaaguacacc acggacaucc ugggcaaccu uuauaaagcu     3120

gccccuccca agaagccuca gcugauuuuc aaauga                               3156


<210>  3795
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3795
auggagaaca agaaucugca cuacauccuc ggccuggacc uugggauugc cagcguggga       60

ugggcugucg uugaaaucga ugagaaagaa aacccccuga gacucauuga cgugggcgug      120

cggaccuucg agcgcgccga aacacagaag ggggaguccc uggcgcuuuc uagacggagc      180

gcucgcuccg ccagacggcu gacgcaacgc agagucgccc ggcucaaaaa ggcuaaacgc      240

cugcugaagu cugagaauau ccuccugagc accgaugaaa gacuuccuca ucaggugugg      300

cagcugcggg uugagggacu cgaccgcaag cuggaaagac aggagugggc cgcugugcuu      360

cugcaccuca uuaaacaucg gggcuaucug ucccaacgca agaacgaguc uaaaagcgaa      420

aauaaggagc ugggggcccu ccuguccgga gucgcgagca accacgaacu ucugcagcag      480

gccacauaca gaaccccggc ugagcucgcc gugaaaaagu uugaaguuga ggagggccau      540

auccggaauc agcaaggcgc uuauacacac accuucucua gacuggaucu ucuggccgaa      600

auggagcucc uguuuucccg ccagcagcac uucgggaacc ccuuugcgag cgaaaagcug      660

cucgagaauc ugacagcccu ucugaugugg cagaaaccug cucucucugg agaagacauc      720

cugaagaugc uuggcaaaug caccuucgag gaugaguaca aggccgcuaa aaacacguau      780

accgccgaac gguuugugug gauuacaaag cugaauaacc ucagaaucca agagaauggg      840

cuggaacgcg cccuguccga caacgagcgg cucaugcuga uugaacagcc cuacgagaaa      900

gcuaaacuua ccuaugcgca ggucagaagc auccugaauc ucucugauga cgccauuuuc      960

aagggagugc gcuacuccgg cgaggauaag aaggcuaucg aaacaaaaac cacgcugaug     1020

gagaugaagg ccuaccauca aauucggaaa gugcuggaag ggaacaaccu uaaggccgag     1080

ugggucgcuc ucaaggccaa uccuacacug cuggacgaga ucggaaccgc guuuagccuc     1140

uauaaaacag augaagacau cuccgcuuac cuggccggca aacuuucuca gcccguucug     1200

aacgcccucc uggagaaucu gagcuucgac aaguuuauuc agcuuucccu caaggcucug     1260

uauaaacugc ucccucugau gcagcaaggc cuuagauacg augaagccug ucgcgagauc     1320

uauggggacc acuacggaaa gaagaccgaa gagacccauc acuuccugcc gcagauuccc     1380

gcugaugaga uccggaaccc uguggugcuc agaacacuga cgcaggcccg caaagucauu     1440

aauggcgugg uucggcugua ugggagcccc gcgagaaucc acaucgaaac cggacgggag     1500

gugggcaagu cuuacaaaga ccgccaugaa cuuaagaaaa gacaggagga aaaccggaag     1560

caaagagaga agacaauuuc cgagaucaaa acccucuuuc cuaauuucag cggggaaccc     1620

aagggaaaag auauucugaa gaugcgccug uacuaucagc agaacgccaa augccucuac     1680

ucuggcaagc cgcuggagcu ucaccggcug uucgaacaga aauaugucga gguggaccau     1740

gcucucccuc ugagcagaac augggaugac uccuuuaaua acaagguucu ggugcuugcc     1800

aaugaaaacc aaaauaaggg caaccucacc cccuucgagu ggcuggaugg gaaaaauaac     1860

ucugagcgcu ggagaacguu uaaggcucug gucgaaacaa gcgccuuccc uuacgcgaaa     1920

aagcagcgga uccucuccca gaaacuggac gagaagggau uuauugaacg caaccuuaau     1980

gauaccagau auguggcccg guuccugugu aacuuuaucg cugacaauau gcaccucacc     2040

ggcgagggga agcgcaaagu cuucgccagc aacggacaga ucacagcucu gcugagacgg     2100

cgcuggggcc uugccaaguc uagagaggac aaugaucggc aucacgcccu ggacgcugug     2160

cucguugcgu gcuccaccgu ggccaugcaa cagaaaauua cacgcuuugu cagauucaag     2220

gcuggggaug uguuuacggg cgaacggauc gacagagaga ccggagaaau uaucccccug     2280

cacuucccca caccuuggca guuuuucaaa caagagguug aaauucgcau cuucagcgau     2340

aacccgaagc ucgagcugga gaaucggcuu cccgacagac cucaggccaa ccaugaauuu     2400

gugcagcccc uguucgucuc ucgcaugccu acccggaaga ugacaggcca ggggcacaug     2460

gagaccguga aauccgccaa gagacucaau gaaggaauua gcaugaucaa aaugccccug     2520

acgaagcuga aacuuaagga ucuggagcuc auggugaacc gcgaacggga gaaggaccug     2580

uacgaugcuc ucaaaaccag acuggaggcc uuuaaugacg auccggcgaa ggcuuucgcc     2640

gaaccuuuua ugaaaaaggg cggggccauc guuaaauccg uccgcgugga gcaagugcag     2700

aagucuggag uccuugugcg gcagggcaac gggguugcug acaaugccag cauggugaga     2760

gucgaugugu ucacaaaaga cggcaaguau uuucugguuc ccauuuacac cuggcaagug     2820

gcgaagggaa uccucccuaa caaagcuguc auucagggca aggacgaaga ggauugggaa     2880

gacaucgaug acgccacauu ccaguuuucc cugcauccca augaucugau uucugugaaa     2940

accaagaaag acgaguuccu uggguacuuu aacggacuga accggcacac gggcgggauc     3000

aauauccgca cacacgaucu cgaaaagagc aagggaaaac agggcauuuu cgaggggauc     3060

ggcguuaaga uugcccuguc cuuugagaaa uaucagaucg acgaacucgg aaagaacauu     3120

agacugugua aaccuagcaa gcggcaaccc gugcgcuga                            3159


<210>  3796
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3796
augaagaaca ccagccugaa uuacgugcuc ggccuggacc uugggaucuc cucugucgga       60

ugggccguug uggagauuga ugaaaaagag aacccccuga gccucaucga cgugggcguc      120

agaacauucg aacgggcuga gacgcagaag ggggaauccc uggcccuugc gcgcagacug      180

gcucggucug cccgcagacu cacccaacgg cgcguggcca gacugaaaaa ggcuaaacgg      240

cugcucaaga gcgagaauau ucugcuuucc acagaugagu gccugccuca ccagguuugg      300

cagcuccgcg uggaaggacu ggagagaaaa cuugaacggc aggagugggc cgcuguccug      360

cuccaucuga ucaaacaccg cggcuaucug ucucaaagaa agaacgagag caaauccgaa      420

aauaaggagc ucggggcccu gcuuagcgga guggcgaaca aucauaaacu gcuccagcag      480

accacauacc ggaccccggc cgaacuggcu guuaagaagu uugaggugga agagggccac      540

auucgcaacc agcaaggcuc uuauacacau accuucucca gacuugaccu gcucagcgag      600

auggaacugc uguuuucucg gcagcagcac uucgggaauc ccuuuacguc cgagaaacuc      660

cuggaaaacc uuaccgcccu gcucaugugg cagaagccug cucugagcgg agaugccauc      720

cuuaaaaugc ugggcaagug uacauucgag aaugaauaca aagcggccaa gcacaccuau      780

ucugcugaga gauuuaucug gauuacaaaa cucaacaauc ugcgcaucca agagaacggg      840

cuggaacggg cccucaauga caacgagaga cuggcucuua ugaagcagcc cuacgaaaag      900

gccaaacuga ccuaugccca gguccgcucc auucucaacc ugagcgauga ggcuaucuuc      960

aagggacugc gguacuccgg cgaagacaaa aagacgucug agacaaaaac cgugcuuaug     1020

gagaugaagg cguaccauca aauuagacuc gcccuggaaa agaaugaucu gaaaacagag     1080

ugggcugaac ucaaggugaa cagcucccug cuugacgaga ucgggaccgc cuuuagccug     1140

uauaaaaccg augaggacau uucuacaugc cucgccggaa agcuguccca gccuguccug     1200

aaugcucugc ucgaaaaccu gaauuucgau aaauuuaucc agcugagccu caaggcccug     1260

cacaagaucc uuccccugau gcagcaaggc cuccgcuacg acgaggcgug ugcugaaauu     1320

uauggcgacc auuacgggaa aaagacggag gaaaaccacc uucugcuucc ucagaucccg     1380

gccgaugaga uucggaaucc cguugugcuc agaacccuga cacaggcccg caaagugauc     1440

aacggagucg ugcggcugua uucuagcccu gcucgcauuc acaucgagac cggcagagaa     1500

guugggaagu ccuaccagga ccggagagag cucgaaaaac ggcaagagga aaaucgcaag     1560

cagagagaga aggccaucuc ugagauuaaa acacuguucc ccaacuuuag cggagaaccu     1620

aaggcuaaag auauccuuaa gaugcggcug uauuaccagc agaaugccaa augccucuac     1680

uccggcaagc cccuggagcu gcauagacuu cucgaacaag gguaugugga gauugaccac     1740

gcgcugccgu ucagccgcac cugggaugac ucuuucaaca auaaaguccu ggugcucgcc     1800

aacgaaaauc agaacaaggg aaaccugacg ccuuuugagu ggcuugaugg caagaauaac     1860

uccgagcggu ggaaggcuuu caaggcccug guugaaacaa gcgcuuuucc cuacaccaaa     1920

aagcagagaa uccucucuca gaaacuggac gagaagggcu ucauugaacg caaucugaac     1980

gauaccagau auguggcccg guuucuuugu aauuucaucg cggacaacau gcaucugaca     2040

ggggagggaa agcgcaaagu cuuugccucc aauggccaaa ucaccgcucu ccugagaggg     2100

cgguggggac ucgccaagag ccgcgaggau aacgacagac accaugcucu ggacgccgug     2160

gucguggccu gcuccacagu uucuaugcag cagaaaauua cgcgguucgu gcgcuuugaa     2220

gagggcaaug ucuucaccgg ggaaagaauc gaucgggaga caggcgaaau uaucccucuu     2280

cacuuucccg cucccuggaa guucuuucgc gaggaggugg aaauuagaau cuucagcgac     2340

aacccuaaac uggagcucga aaaucggcug auugcgagac cgcaagccaa ccacgaguuc     2400

guucagcccc uguuuguguc ccgcaugccu acccggaaga ugacaggaca gggccauaug     2460

gaaaccauca agucugcuaa aagacuuaau gaggggcuga gcgucaucaa gaugcuccug     2520

acgaaacucc gccugaagga ucuugagucc auggugaacc gggaaagaga gaaagaacug     2580

uacgaggccc ucaaggcccg ccuggaggcu uucaaugacg aacccgccaa ggcguuugcu     2640

gagccuuuca ugaaaaaggg aggcgccauu gugaaaagcg uucgggucga acaggugcaa     2700

aagucugggg ugcuggucag agauggaaac ggcguggccg acaacgcuuc caugguucgc     2760

guggaugucu uuaccaaagg gggcaaguau uuccuugugc ccaucuacac auggcagguu     2820

gccaaaggca uucugccgaa uaaggcggug auccagggaa agaacgagga agacugggag     2880

gucauggaug acgcuaccuu ucgguucagc cuccaccaga augaucuggu gaaacucguu     2940

acaaagaaaa agaccauuuu uggguauuuc aacggacuga auagagccac gggcaacauc     3000

gacaucaaag agcaugaucu ugaaaagucu aaggugaaag acgggauuca ccaauccauc     3060

ggaauuaagc uggcccugag cuuugagaaa uaccaggucg acgaacucgg caagaauauc     3120

cggcugugua aaucuagcaa gcgccaggcu gugagauga                            3159


<210>  3797
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3797
augaucggcg agcacgugag agggggaugc cuguucgacg aucauuggac ccccaacugg       60

ggcgccuuuc ggcucccuaa uacaguccgc acguucacca aggcugaaaa cccgaaagac      120

gggagcuccc uggccgagcc cagacggcag gcgcgcggac uuagacggcg ccugagacgg      180

aagacacaac gccucgaaga ucugagacgg cuucuggcua aagagggcgu ucucucucug      240

agcgaccugg aaacccucuu ucgcgagacc ccugccaagg aucccuacca gcugagagcc      300

gaggggcuug accggccucu guccuucccc gaaugggugc gcgugcucua ucacauuaca      360

aaacauagag gauuucaguc uaaucggaga aacccugucg aggauggcca ggaacgcagc      420

cggcaagagg aggaaggcaa gcugcuuucc ggggugggag agaaugaaag acugcuccgc      480

gagggcgggu accggacagc uggagaaaug cuggccagag acccgaaguu ccaggaucac      540

cgccggaaca gagcuggcga cuauucucau acccugagcc gcucccuccu gcuugaggag      600

gcccggagac uguuucagag ccagcgcacg cucgggaauc cccacgcguc uuccaaccug      660

gaagaggccu uccuucaccu gguugcuuuu caaaauccuu ucgccagcgg agaagauauc      720

cggaacaaag cuggccauug uucucucgag cccgaccaga uuagagcccc ucggcgcucc      780

gcgagcgccg aaaccuuuau gcugcugcag aagacaggca aucucagacu gauccaccgg      840

agaaccgggg aggagcgccc ccuuacagau aaagaacggg agcagauuca ucugcucgcu      900

uggaagcaag aaaaagugac ccacaagacg cugagacgcc accuugagau cccggaagag      960

uggcuguuca caggacuccc uuaccauaga ucuggcgaca aggccgagga aaaacuguuu     1020

guccaccugg cugggaucca ugagauucgg aaggcccucg acaaaggacc cgauccugcc     1080

gugugggaca cccugcgcuc cagacgggau cuucuggaca gcaucgcuga uacacucacc     1140

uucuauaaga acgaagacga gauucugccc cgccuggagu cccuuggccu cucucccgaa     1200

aaugcgagag cccuggcucc ucugagcuuc uccgggaccg cccaccucag ccugucugcc     1260

cuuggaaaac ugcucccgca ucuggaggaa ggcaaguccu acacacaggc ucgggccgau     1320

gcgggcuaug cugcuccccc ucccgaccgc cacccuaaac ugcccccgcu ugaggaagcc     1380

gauuggagaa acccuguugu guuucgggcu cucacgcaga cccgcaaggu cgugaaugcc     1440

cuggugagac gguacgggcc cccuuggugc auccaccugg agacagcuag agagcucagc     1500

caacccgcca agguccgccg gagaauugaa accgagcagc aggcgaacga gaaaaagaaa     1560

cagcaagccg aacgcgaguu ccuggacauc guuggaacag cuccuggccc cggggaucuu     1620

cugaagaugc ggcucuggag agaacaggga ggcuuuuguc cguacugcga ggaauaucug     1680

aacccuaccc gccuggccga gcccggguac gcugaaaugg accauauucu ucccuauucu     1740

cggagccucg acaauggcug gcacaacaga gugcuggugc auggaaaaga uaaucgcgac     1800

aagggcaacc ggacgccuuu cgaggccuuu gggggagaua cagcgagaug ggaccggcug     1860

gucgccuggg ugcaggcuuc ccaccucucu gccccgaaga aacgcaaucu gcuuagagaa     1920

gauuucggcg aggaggcuga acgggagcug aaggaccgca accucaccga uaccagauuu     1980

aucacaaaaa ccgccgccac acugcugcgg gacagacuua cguuccaccc cgaagcuccu     2040

aaggaucccg uuaugacccu caaugggcgc cugacagcgu uucugcggaa acagugggga     2100

cuccauaaga acagaaagaa uggcgaccug caccaugccc uugaugcugc cgugcuggcc     2160

gucgcuagcc gcuccuucgu guaccggcuc agcucucaca acgccgcgug gggagagcug     2220

ccuagaggcc gcgaggcuga aaauggauuu ucccugcccu auccugccuu ccggagcgag     2280

guucuugcca gacugugucc cacccgcgaa gagauccucc ugagacucga ccaaggcggg     2340

gugggauacg augaagccuu ucggaacggc cugcgcccgg ucuucguguc uagagccccu     2400

ucccggcgcc uuagagggaa agcgcauaug gagacacugc ggagccccaa guggaaagac     2460

cacccugagg gacccagaac cgcuucccgc auuccgcuca aggaccugaa ucuggaaaaa     2520

cuugagcgga uggucggcaa ggauagagac cgcaagcugu augaagcccu ccgggagaga     2580

cuggccgcuu ucgggggcaa cgggaaaaag gccuuugugg cuccuuuccg gaaacccugc     2640

cgcucuggcg aagggccucu cguuagaagc cugcggaucu uugauuccgg auacucuggc     2700

guggagcuuc gcgacggggg agaggucuac gccguggcgg aucacgaaag caugguuaga     2760

guggacgucu augccaagaa aaaucgguuc uaccuggugc ccguguaugu ugcugauguc     2820

gcccgcggca uugugaagaa cagagcuauc guggcccaua aauccgagga agagugggac     2880

cucgucgaug ggagcuuuga cuuccgguuu ucucuguucc ccggcgaucu gguggaaauu     2940

gagaagaagg acggagccua ccuuggcuau uacaaauccu gucaccgcgg ggauggaaga     3000

cugcuccugg accggcauga cagaaugccu cgcgagagcg auugcggcac guuuuauguu     3060

ucuacccgga aggacgugcu cuccaugagc aaauaccagg ucgauccgcu gggggaaauc     3120

agacuugugg gaucugagaa gcccccuuuc guucuguga                            3159


<210>  3798
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3798
augaagagaa acuacauccu gggccucgac auugggauca ccagcguggg auauggcauu       60

aucgauuacg agacacggga cgucauugau gccgggguuc gccuguucaa agaagcuaau      120

guggagaaca augaaggaag acgguccaag cgcggcgcca gacggcuuaa acgcagacgg      180

cgccacagaa uccagcgggu gaagaaacug cucuuugacu auaaccugcu uacggaucau      240

ucugagcuga gcgggaucaa ucccuacgaa gcgcgcguca agggacucuc ccaaaagcug      300

ucugaggagg aguucagcgc ugcccugcuc caccuggcca aaagacgggg cgugcauaac      360

guuaaugaag uggaagagga caccggcaac gagcuuucca caaaggaaca gauuucucgc      420

aauagcaaag cucuggagga aaaguauguc gccgagcucc agcuggaaag acuuaaaaag      480

gauggggagg ugcggggauc caucaacaga uuuaagacca gcgacuacgu uaaagaggcu      540

aagcagcugc ucaaagugca aaaggccuau caccagcugg aucagucuuu cauugacaca      600

uacaucgauc ugcucgaaac ccgccggaca uacuaugagg gcccugggga aggauccccg      660

uuuggcugga aagacauuaa ggagugguac gaaaugcuga uggggcauug caccuauuuc      720

cccgaggagc uuagaagcgu caaauacgcg uauaaugccg aucuguacaa cgcucucaau      780

gaccugaaca aucuugugau cacgcgcgac gaaaacgaga agcuggaaua uuacgagaag      840

uuucagauua ucgaaaacgu guucaaacaa aagaaaaagc cuacccucaa acagaucgcc      900

aaggagauuc uggucaauga ggaagauauc aagggauacc ggguuacauc uaccggcaaa      960

cccgaguuua caaaccugaa gguguaucac gacauuaaag auaucaccgc uagaaaggaa     1020

auuaucgaga augccgagcu ccuggaccag aucgcgaaaa uucuuacgau cuaccagucc     1080

agcgaagaua uucaagagga acugacaaac cucaauucug agcugaccca ggaagagauc     1140

gagcagauuu ccaaccuuaa gggcuauaca gggacccaca aucugagccu caaggccauc     1200

aaccugaucc uggacgaacu cuggcauacc aaugauaacc aaauugcuau cuucaaucgc     1260

cugaaacuug ugccuaagaa agucgaccug ucccagcaga aggagauucc cacaacgcuc     1320

guggaugacu uuauccuguc uccuguugug aaacggagcu ucauucaguc caucaagguc     1380

auuaacgcca ucaucaagaa auacggacug ccgaaugaua uuaucauuga acuugcuaga     1440

gagaagaaca gcaaagacgc ccaaaagaug aucaaugaaa ugcagaaacg caaccggcag     1500

accaacgaga gaauugagga aaucauccgc acaaccggca aggagaaugc caaauaucuc     1560

auugaaaaga ucaagcugca cgacaugcag gaggggaaau gucuguacuc ucucgaagcu     1620

auuccccugg aggaucuucu gaacaauccu uuuaacuaug agguggacca uaucauuccc     1680

cgguccguua gcuucgauaa uucuuucaac aauaaggugc ucgucaaaca agaagagaac     1740

agcaagaaag gaaauagaac accuuuucag uaccuguccu cuagcgacuc caagaucagc     1800

uacgaaaccu ucaagaaaca cauccugaac cuugcgaagg gcaaagggcg gauuucuaag     1860

acgaaaaagg aguaucuccu ggaggaacgc gauaucaaua gauuuuccgu gcagaaggac     1920

uucauuaacc ggaaucuggu cgauacaaga uacgccaccc gcggacucau gaaccugcuu     1980

cggagcuauu uuagagugaa uaaccuggac guuaaaguga agucuaucaa cggcggcuuc     2040

accuccuuuc uccgcagaaa auggaaguuc aaaaaggagc ggaauaaggg guacaaacac     2100

caugcugaag augcccugau uaucgccaac gcugacuuua ucuucaagga guggaaaaag     2160

cuggauaaag ccaagaaagu cauggaaaau cagauguuug aggagaagca agcggaaagc     2220

augcccgaga uugaaacaga gcaggaauau aaggagaucu ucauuacccc gcaccagauc     2280

aaacauauua aggacuuuaa agauuacaag uauucccacc gcguggacaa aaagccuaac     2340

agagagcuua ucaaugacac acucuacucu acgcggaagg augacaaagg aaacacccug     2400

auuguuaaua accugaaugg ccucuacgau aaggacaacg auaaacugaa gaaacuuauc     2460

aauaagagcc ccgaaaagcu gcucauguau caucacgacc cucagacaua ccaaaaacug     2520

aagcugauca uggagcagua uggggaugag aaaaaccccc uuuacaagua uuacgaagaa     2580

accggaaauu aucucacaaa auacuccaag aaggacaacg gccccgugau uaaaaagauc     2640

aaauacuaug ggaauaagcu gaacgcucac cuggauauua ccgacgauua cccuaacucu     2700

cgcaauaaag ucgugaagcu cagccugaag ccguauagau ucgacgugua ccuugauaac     2760

ggcguuuaua aauucgucac ggugaagaau cuggacguga ucaaaaagga gaacuacuau     2820

gaggucaauu ccaaaugcua cgaagaggcc aagaaacuca agaagauuag caaccaggcc     2880

gaauuuaucg cuucuuucua caauaacgac cugaucaaaa uuaauggaga gcuguaucgg     2940

gugaucggcg uuaacaauga ucuucugaac cgcauugagg ugaauaugau cgacauuacc     3000

uacagagaau aucucgagaa caugaaugau aagcggcccc cucgcaucau caaaacaauu     3060

gccuccaaga cccaaagcau caaaaaguac ucuacagaca uucuggggaa ccucuaugaa     3120

gucaagucca aaaagcaucc ccagaucauu aaaaagggau ga                        3162


<210>  3799
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3799
augaagagaa acuacauccu gggccucgac auugggauca ccagcguggg auauggcauu       60

aucgauuacg agacacggaa ugucauugac gccgggguuc gccuguucaa agaagcuaac      120

guggagaaua acgaaggaag acgguccaag cgcggcacga gacggcuuaa acgcagacgg      180

cgccacagac ugcagcgggu gaagaaacuc cuguuugauc auaauuuccu uaccgacaac      240

ucugagcuga gcgggaucuc ccccuaugaa gcccgcguca agggacucaa ucaaaagauc      300

ucugaggagg aauuuagcgu ggcgcugcug caccucgcua aaagacgggg cguucauaac      360

gugaaugagg ucgaagagga uacaggcaac gagcugucca ccaaggaaca gauuucucgc      420

aauagcaaag cccuugagga aaaguacgug gccgagcugc agcucgaaag acugaaaguu      480

gacggggagg ugcggggagc ugucaacaga uucaagacau ccgauuaugu gaaggaggcc      540

cagcaaaucc uuaaagugca gaaggcuuac caccaguuug accagagcuu cauugauacc      600

uauaucgacc ugcucgaaac acgccggacc uacuacgagg gcccugggga aggaucuccg      660

uuuggcugga aagauauuaa ggagugguau gaaaugcuga uggggcaaug cacguacuuc      720

cccgaggagc ugagauccgu caaauaugcc uacaaugcgg accucuauaa cgcccugaau      780

gaucuuaaca accugguuau cgcucgcgac gaaaaugaga agcucgaaua cuaugagaaa      840

uuucagauua ucgaaaacgu guucaagcag aagaaaaagc cuacccugaa acagaucgcc      900

aaggagauuc uugugaauga ggaagacauc aaaggauacc gggucacaag caccggcaag      960

cccgaguuua caaacuucaa gguguaccau gauauuaaau cuaucaccuc cagaaaggaa     1020

auucuggaga augcugagcu ccugaaccaa aucgccaaaa uccugacgau uuaucagagc     1080

ucugaagaca uccagggcga gcucgcgcgc cuguacuccg aacuuacaca agaggaaauu     1140

gagcagauca gcaagcuggc cggguauacc ggaacacacc ggcucucccu gaaagcuauu     1200

aaucuuauca ucgaugagcu guggcacacc ucugacaacc agauggaaau uuuuaauaga     1260

cucaagcugg uuccuaagaa aguggaucug agccagcaaa aggagauccc caccacacuc     1320

gucgacgauu ucauucuguc cccugugguu aaacgcagcu ucauccaguc uauuaaggug     1380

aucaacgcca uuaucaaaaa guacggcuuu ccgaaugaca ucauuaucga acuugcucgg     1440

gagaagaacu ccaaagaugc ccagaaguuc auuaaugaaa ugcagaaaag aaaccgccaa     1500

acgaaugagc ggaucgagga aauuaucaga accacaggga aggagaacgc caaauaucug     1560

aucgaaaaga uuaaacucca ugacaugcag gagggaaagu gucuguacag ccuggaagcu     1620

aucccccuug aggaucuccu gaacaauccu uuuaacuaug aggucgacca cauuaucccc     1680

cggucuguga gcuucgacaa uuccuuuaac aauaaggucc uggugaaaca ggaagagaac     1740

ucuaagaaag gcaaucgcac cccuuuccag uacuuuagcu ccagcgauuc uaagauuucc     1800

uacgaaaucu ucaaaaagca uauccucaac cuggcgaagg ggaaaggaag aauuagcaag     1860

aaaaagaaag aguaucuucu ggaggaacgg gacaucaaua gauuuucugu ucaaaaguac     1920

uucauuaacc gcaaucucgu ggauacacgg uaugccacca gagagcugau gaaccugcuu     1980

cgcuccuacu uuagagucaa ugaccucgau aucaagguga aaagcauuaa cggcggcuuc     2040

acguccuuuc ugcggaagaa auggaaguuc aaaaaggaac gcaauaaggg guauaaacac     2100

cacgcugagg acgcccugau caucgccaac gcugauuuca uuuuuaagga auggaaaaag     2160

cucgacaaaa caaacaaggu uauggaggau cagaucgugg aggaacagca ggccgagucu     2220

augcccgaaa uugagaccga acaagaguac aaagagaucu ucauuacccc gcaucagauc     2280

caggacauua aggauuuuaa ggacuauaaa uacagccaca gagucgauaa gaaaccuaau     2340

cgggaacugg ugaacgacac acuuuacucc acccgcaagg acgauaaagg aaauacacug     2400

aucgugaaca aucucaaggg ccuguaugac aaggauaacg acaaacugaa gaaacuuauc     2460

aauaagucuc ccgagaaacu ccugauguac caucacgauc cucaaacgua ucagaagcug     2520

aagguuauua uggaacagua cagcaacgag aaaaaucccc ucuauaagua ccaugaagag     2580

accgggaacu aucugacaaa auacuccaag aaagacaaug gacccgucau caagaagauu     2640

aaauacuaug gcaacaaacu uaauguguac cuggauauca ccagagacua ucucaagagc     2700

cggaacaaga uugugaaacu gucucugaag ccuuaccgcu ucgaugucua ucuugacaau     2760

gggguguaca aguuuguuac agugaaaaac cucgauauca ucaagaaaga ggacuauuac     2820

gaagucgauu ccgacagcua cgaggaagcg aagauuguga aaaagguuuc ugagcaggcu     2880

caauucgugg ccuccuuuua uaacaaugac cugaucaaag ucgauggcga gcuguucaga     2940

gugauuggag uuaacaauga ccuccugaac cggaucgaag ugaauauggu cgauauuacc     3000

uacaaggagu aucuugaaaa caucaaugac aagaaaagcu cucgcaucau uaagacgauc     3060

agcuccaaaa cccagagcau uaagaaauac ucuacagaua uccugggcaa ccucuaugag     3120

gccaagucca agcagagacc gcagaugacc augaaagggu ga                        3162


<210>  3800
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3800
augaagagaa acuacauccu gggccucgac auugggauca ccagcguggg auauggcauu       60

aucgauuacg agacacggga cgucauugau gccgggguuc gccuguucaa agaagcuaau      120

guggagaaca augaaggaag acgguccaag cgcggcgccg ggagacuuaa acggcgcaga      180

cggcaccgcc ugcagagagu gaagaaacuc cuguuugacu auaaccuucu gacggaucau      240

ucugagcuca gcggaaucaa ucccuacgaa gcgcggguca agggccuguc ccaaaagcug      300

ucugaggagg aguucagcgc ugcccuccug caccuugcca aacgcagagg cgugcauaac      360

guuaaugagg uggaagagga caccgggaac gagcugucca caaaggaaca gaucucucgg      420

aauagcaaag cucucgagga aaaguauguc gccgagcugc agcuugaacg ccugaaaacc      480

gauggagagg ugagaggcuc cauuaaccgg uuuaagacaa gcgacuacgu uaaggaggcu      540

aaacagcucc ugaaggugca aaaagaauau caccagcugg aucagucuuu caucgacacc      600

uacauugauc uccuggagac aagacgcacc uacuaugaag ggccuggaga gggcuccccg      660

uuugggugga aggacaucaa agaaugguac gagaugcuua ugggacauug cacguauuuc      720

cccgaggaac ugcggagcgu caaguacgcc uauaaugcgg aucucuacaa cgcccugaau      780

gaccuuaaca aucuggugau ugcuagagac gagaacgaaa aacucgagua uuacgaaaag      840

uuucagauca uugagaacgu guucaagcaa aagaagaaac cuacccugaa gcagaucgag      900

aaagaaaucc uggucaauga ggaagauauu aagggcuacc gcguuacauc uaccggcaag      960

cccgaguuuu ccaaccucaa aguguaucac gacaucaagg auauuacagc ccggaaagag     1020

aucauugaaa augcugagcu gcuugaccag aucgccgaaa uccugaccau uuaccagagc     1080

ucugaggaua uccaagaaga acucgugaag cugaacuccg agcuuacgca ggaagagauu     1140

gaacagauca gcaaucugaa aggguauaca ggaacccaca accucucccu gaaggcgauu     1200

aaucugaucc ucgacgagcu guggcauaca aacgauaauc aaauggccau cuucaacaga     1260

cuuaagcugg ucccuaaaaa gguggaccuc ucucagcaga aagaaauucc caccacccug     1320

guugaugacu uuauccugag cccugugguc aagcgcuccu ucauucagag caucaaagug     1380

auuaaugcua ucauuaagaa guacggccuu ccgaacgaua ucaucauuga gcucgcccgg     1440

gagaaaaauu cuaaggacgc ucagaaaaug aucaacgaaa ugcaaaagag aaaucgccag     1500

acaaacgagc ggauugaaga gaucauuaga acgaccggga aagaaaacgc caaguaucug     1560

aucgagaaaa ucaagcugca cgacaugcag gagggaaagu gucucuacuc ccuggaagcc     1620

auuccccuug aggaucugcu caauaacccu uuuaauuaug aaguugacca uaucauuccc     1680

cgcagcgugu cuuucgauaa cagcuucaau aacaaagucc uggugaagca agaggaaaau     1740

uccaaaaagg gcaaccggac accuuuucag uaccugucua gcuccgacag caaaaucucu     1800

uacgaaaccu ucaagaagca cauucuuaau cucgcuaaag ggaagggaag aaucuccaaa     1860

acaaagaaag aguaucugcu ggaggagcgg gauaucaacc gcuuuagcgu ccagaaggac     1920

uucauuaaua gaaaccucgu ggauacccgg uacgcgacga gagaacugau gaaucuucug     1980

cgcucuuauu uucggguuaa caaucucgac gugaagguca aauccaucaa cggcggcuuc     2040

accagcuuuc ugagacgcaa guggaaauuc aagaaagaga gaaacaaggg guacaagcac     2100

caugccgagg augcucugau uaucgccaau gccgacuuua uuuucaaaga auggaagaaa     2160

cuugauaagg cuaaaaaggu gauggagaac cagacaguug aagagaaaca agccgaaucc     2220

augucugaga ucgagaccga acaggaguau aaggaaaucu uuauuacacc ccaccagauc     2280

cagcauauua aggacuucaa agauuacaag uauagccacc ggguggacaa aaagccgaau     2340

cgcgagcuca ucaacgacac ccuguacucc acaagaaaag augacaaggg aaauacgcug     2400

auugucaaca aucucaacgg ccuguaccau aaggauaaug acaaacuuaa gaaacugaug     2460

aacaagucuc cugaaaaacu ccugauguau caccacgauc cccaaaccua ccagaagcug     2520

aaacuuauca uggagcagua uggggacgag aaaaauccuc ucuacaagua uuacgaagag     2580

acaggaaacu aucugaccaa auacagcaag aaagauaaug gccccgugau uaagaagauc     2640

aaauacuaug ggaacaagcu gaaugcgcau cucgacauca cagaugacua ccccaacucc     2700

cggaacaaag ugguuaagcu gagccuuaaa ccuuaucgcu uugaugucua ccuggacaau     2760

ggcguguaua aguucgugac cgucaagaac cucaauguga uuaaaaagga aaacuacuau     2820

gagguuaauu cuaaaugcua cgagaaggcu aaaaagcuga aaaagaucuc cgaucaagcc     2880

gaguucauug ccagcuuuua caacaaugac cugaucaaga uugacggaga acuuuauaga     2940

gugaucggcg ucaacacgga ucugaucaau cggauugaag ugaacauggu ugacaucacc     3000

uaccgcgagu aucucgaaaa uaugaacgau aaaagaucuc cgcggauuuu caagacaauc     3060

gcuuccaaaa cccagagcau uaagaaauac ucuacagaca uccuggggac ccucuaugag     3120

gugaauagca agaagcaccc ccagaugauc augaaaggau ga                        3162


<210>  3801
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3801
augagcggca ugguguucgg gaucgaccug ggaauugccu ccugcggcug ggcuguccuc       60

agacagcccc aacgggaugg ggacccugga gagaucguug aucugggcuc uuggauguuu      120

gacgugccgg aaaccgauaa ggagcgcaca cccacgaacc aggugagacg ggggaaucgc      180

cuucugagac gggucauucg cagacgggcc cagcgcaugg uggaaaucag acgccucuuc      240

cacgaccaug gacugcuugc gggccacgcu ccugaggccc ugaaacgggc cggccucgau      300

cccugggacc ugagagcucg gagccuggau aagguucucg aaccugccgc ucuggccgug      360

gcgcuugggc auauugccaa acgcagagga uuuaaguccg cugcccggag aaaagaggcu      420

aacaccgccg gcgacgauca gaagaugcug aaggcgcucg aggccacaca cgaacgccug      480

gggcgguaca gaaccaucgg agagauguuc gcucgcgacc ccgauuuuga aucucggaaa      540

agaaaucgcg acggcauguu cgaccggaca caagggagag augaccuucu gcaugagguc      600

ggagagaucu uuaaggccca gcgccggcuc ggcagcgcuc uggccaccgc cgaacuggag      660

caggcuuuca cagcgauugc cuuuagacag cucccuaucc aagauuccga acgccuggug      720

ggccuuuguc aguucgagcc gaaagaaaag cgggcugcca gauuugcccc cucuuucgag      780

cgguuucgcc ugcuccagag acugaccaac cuucggguug ugacggucga gggggaaaga      840

ccucugaccg cugacgagau ugccgcggcu gccgccgauc ucggacgcac agcuaaacug      900

agcgugaagg aagugcggaa gagaaucggc cuggccgcug accaccgcuu cgccgcguuc      960

aaagccgaug aggaagacag agauauuauc gcucggaccg gggaggcccu ccacggaaca     1020

uaucgccuga gaaaggcucu uggcgagggg cugugggccg aaaugcuccc cggacagcug     1080

gacgcgauug cccaugcucu uuccuuuuuc gagacccaag augucauccu gaaagaacuc     1140

gacaagcugg accugccugc cggcguucgg gaugcuaucg ccacgggccu cgacgccggg     1200

gcuuuugcgc gcuucaaagg aacaggccac auuagcgcca gagcugcccg ggcccugcuu     1260

ccccaucugg aggcugggcu ccgcuacgau caggccugca ccaaggcggg auaugaccac     1320

gcugccucua gaugggccaa acgggaacag guggcugaua aggccgcguu uaaucgccug     1380

gugacagaca ugggcgcuga gaucgccaac ccgauugcca gaaagucccu gaccgaggcu     1440

cuuaaacaac ucugggccau gcggaauaga ugggggcugc cugaugcuau ccacauugaa     1500

cuggcccgcg acgucggcaa cagccucgag aagcggagag aaaucgagcg cgcgaucgag     1560

aaaaauaccg ccgcucggga aagagagcgc ggagaagccc gggaucugcu uggcauugac     1620

gaugugucug gggacacacu gcucagauac cgccugugga aggagcaggc uggacggugu     1680

cccuauacgg augccccuau cccucccggc gccauuaucg cuaccgacaa cuccuuccag     1740

guugaccaua uucugcccug gagcagauuu ggggaugacu cuuucgcgaa uaaagugcuu     1800

ugcgccacag cugccaacca gcggaagaag ggacaaaccc cgugugaaug gaucacagcc     1860

gcucagggcg aggaggggug ggccaccuuu gucgcgcgca ucgaaggcaa ugcugccuuc     1920

agaggaccca aaaagcggaa cuacgugcuc aaaaaugcca aggaggcuga ggaacgcuuu     1980

agagcccgga accugaacga uacgagauau gcggcucgcc ugcucgccga agccguuaaa     2040

cuguucuacc cugagggcga gcggcaggac aaagggggag ugagacgcgu cuuuacacgg     2100

cccggcgggc uuaccgcugc ccugagacac gcuuggggag uggaagcccu caagaagcgc     2160

gauggcaagc gggucgacga ugcgagacau cacgcccugg acgcucuggu gguugccgcu     2220

auuggggagg gcgaagugca gcgccuuacc agauccuacc aagaguggga acagcaggga     2280

cucgcccggc cucugcgcag agucgauccc ccguggggcg acuuccauag cuuccggcgc     2340

gaggugaaag augccuauga cgggaucuuu guugcuagac cugagcggag acgcgcgcgg     2400

ggagaaggcc acgccgcuac aauuagacag gugcgcgagc gggauggggc cgccgucgug     2460

uucgaaagaa aggcuaucgc cgaccugucc gagaaacggc ucgcggacau uaaggauccc     2520

gagcgcaauc aagcuaucgu ggaagccauu agacagugga ucguugacgg acggccugcc     2580

gaucgccugc ccagaucucc ugcuggcgac gagauccgga agguccgccu uagaaccaaa     2640

gggaagcccg ccguccaggu gcggggcgga gcggccgauc gcggcgaaau ugugagagug     2700

gacguuuuua caaaaccgaa caagaaaggg aagaaugagu ucuaccuggu gccuaucuau     2760

ccccaccagg ucaugaacaa agcugaaugg cccacgccuc cgaugcgggc cgugguugcu     2820

uacaaggaug agagcgagug gacccuccug gacgaaaauu uuggauuccu guuuucccuu     2880

uuccccagaa gcuaugugga ggucacaaag ccuggcgggg aagugcucuc uggauacuuu     2940

caaggcaugg aucgcuccac cggcgccauu agccuguuca accaucggga cucuagaagc     3000

cugacagaug acuccggaaa uucuacccgc gggaucggcg cuaaaacgcu ccugaccaug     3060

aagaaauaua gcguugaccg guuugggaag agagccgagg ugaaauccga aguccgcaca     3120

uggcacggag uggcgugcac cuaccccaca ccucccggcu ga                        3162


<210>  3802
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3802
augaagcuga caccccucaa cuacauccug ggccuugacc uggggauugc cagcguggga       60

ugggcugucg uugagaucga ugaacaggag aauccucucg gccugauuga cgugggggug      120

agaaccuucg aucgggccga agucgcgaaa acgggagagu cccuugcucu ggcccgcaga      180

cucgcccggu cuagccgcag acuggugaag cggcgcgcug acagaaucaa aaagguuaaa      240

cggcugcuca aguccgaaaa cauucugcuu ucugccgaug agcaacugcc gaaugacgug      300

uggcagcucc gcgucaaggg ccuggaucag aaacuugaga gacaggaaug ggcugccgug      360

cugcuccacc uucugaagca ucggggguau cucagccaac gcaaaaacga guccaagucu      420

gaaaauaaag agcugggagc gcuuagaagc ggcguugccg cuaaccacca gauccugcag      480

uccgccgagu accggacccc cgcugaaauc gccgugaaga aguuucaugu cgaggaaggc      540

cacauucgca aucagagagg ggcguauaca cauaccuuca gccggcucga ccugcuugcc      600

gagaucgaac ugcucuuuac aagacaaacc gagcugggaa acccucacac aucugcuaaa      660

cugcucgaga aucugaccgc ccuucugaug uggcagaagc ccgcucucgc cggcgaagcc      720

auucugaaaa ugcuugggaa gugcacguuc gagccuaccg aauacaaagc ugcgaagaac      780

uccuauagcg ccgagcgcuu uguguggcug acaaaacuca auaaccugcg gauccuggaa      840

cagggagcug agagagcccu caccgauaau gagcgcuucg cccugcuuga ccagcccuac      900

gaaaaggcua aguuuacaua ugcccaagcg cggaccaugc uggcucuccc ugaugaggcc      960

auuuucaaag gcgugagaua ccagggggaa gacaagaaag ccgucgagac gaagacaauc     1020

cugauggaaa ugaaggcuua ccaccagauu cgcaaagccc uggagaacgc ugaucuuaag     1080

gccgagugga augaacucaa aaacaacucu gagcugcugg acgacaucgg aaccgcguuu     1140

ucccuccaua agacagauga agacaucugu cgguaucugg auggcaaacu uagcgagucu     1200

auucugaaug cccuccugga gaaccugaau uucgacaagu uuauccaacu uucccucaaa     1260

gcucugcagc agauucugcc gcucaugcug cagggccaaa gauacgauga agccguuagc     1320

gcuaucuaug gggaccacua cggaaagaag uccgccgaga uuaaccgccu ucugcccacc     1380

aucccugcgg augaaauccg gaaucccgug gugcucagaa cccugacaca ggcccgcaaa     1440

gucauuaacg cugugguucg gcuguauggc ucuccugcca gaauccauau ugagacgggg     1500

cgggaagugg gaaagagcua ccaggaccgc aaaaagcugg agaaacagca agaggauaau     1560

agaaagcacc gggaauccgc uguccagaag uucaaagagu auuuucccaa cuucgugggc     1620

gaaccgaagg ggaaagacau ccucaagaug agacuguacg agcugcagca ggccaaaugc     1680

cucuacagcg gaaagucucu ggaacuucac cgccugcucg agaaaggcua uguugaggug     1740

gaucaugccc ugccuuucuc ccggaccugg gacgacagcu uuaauaacaa gguccuggug     1800

cuugcuaaug aaaaccaaaa uaagggcaac cucacacccu acgaguggcu ggaugggaaa     1860

aauaacucug aaccuuggca gaacuucguc gcgagagugc agaccagcgg auuuucccac     1920

acaaagaaac agcgcauucu gucucauaag cucgacgaga aaggcuucau cgaaagaaau     1980

cugaacgaua cccgguaugu ugugcgcuuu cuuuguaauu ucaucgccga caacaugcug     2040

cucacgggga agggaaagag aaaagucuuu gcuagcaaug gccaaauuac agcccugcug     2100

cgggggcgcu ggggccuuca gaaggugaga gaugacaacg aucggcacca ugcccuggac     2160

gcuguugugg ucgccugcuc caccguggcg augcagcaga aaaucacccg cuucgugaga     2220

uacgaggagg gaaauguuuu uagcggcgaa cggauugauc gcgagacagg ggaaaucauu     2280

ccccuccacu uccccucucc uugggccuuu uucagagaga acgucgagau ccggaucuuu     2340

uccgaaaauc cgaagcugga gcucgaaaac agacugcccg acuauccuca auacaaucac     2400

gaguucgugc agccccuuuu cgugagccgc augccuaccc ggaaaaugac aggacagggc     2460

cauauggaaa cggucaaguc ugcuaagaga cuggaugagg ggcucuccgu gcugaaaguu     2520

ccccugaccc aacuuaagcu gaacgaccuc gagcgcaugg ugaaucggga aagagagaug     2580

gcccuguaug aaagccucaa aguccgccug gagcaguuug auaacgaccc ggcuaaggcc     2640

uucgcggaac cuuuuuacaa aaagggaggc gcucuuguga aggccguucg gguggagcag     2700

acacagaaau ccgggguccu ggugagagac ggcaauggag uugccgauaa cgcuucuaug     2760

gugcgcgucg acguguucac caaggccggc aaauacuuuc ucgugcccau uuauacaugg     2820

caagucgcua aggggauccu gccuaacaaa gccguuaccg cgaaugugga ugagauugac     2880

uggcuggaaa uggaugagag cuaccaguuc aucuuuacga uguaucccaa cgaccuugug     2940

aaggucaaac ugaagaagga agaguucuuu ggauacuaug gcgggcucga ucgggccacc     3000

ggagcuauug ugaucaaaga acacgaccug gagaagucca aaggcagaca ggggauuuac     3060

cggaucggcg uuaagcucgc ccugucuuuc gagaaauauc agguggauga acuuggaaag     3120

aauauccgcc cuuguagacc cacaaagcgg caacaugucc gcuga                     3165


<210>  3803
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3803
augagaaucc ugggcgugga ccucgggguc gccagcguug gaugggcucu ggugaacgau       60

aagaauaaca aaauuaucga cuccggcgug cggauuuuca cccagucuac aaaucgcgag      120

gggaagacgc uugccgcggu cagacggagc uuucgcggag gcagacgggu gcugucccgc      180

agaaaacaac ggcucaacca gcugaagaaa cuuuucaucg cucacaagcu gcucucuaag      240

agcgaauuua aacaucuguu caauuccaag aaccgccugg auauuuggca gcucagagcc      300

aaagggcugg agcagcaacu uaauaacaag gaaugggccc ggguucugua ccacaucgcu      360

aaacgcagag ccuaucaguc uaaucggaag uccgaggaau ccggaaacac cgacaagaaa      420

aaggugcuca gcggcaucuc uaaaaaugag aagaaaauga acgagcuggg cuacaagaca      480

auuggggaaa ugcuuuauac cgagaaucgc uaccccgaua acagaauccg gagaaauaaa      540

aaggaccagu auaagcauuc cauuagccgc gaucugcucc ggaaagaaau cgacguccug      600

uuugagucuc agagagauuu cggaaaccaa uuugcuacag acggccugaa ggagcacuac      660

aaagauauug ccuucaccca gcgcccuauc aaguuuaaag cggaccucgu ggggaagugc      720

acauucgaaa cccaggagua ucggggagcc aagaacuguu uuuccguuga aaagagcacg      780

cugcuuucua agauuaauaa caccgaucug aucgacaaau ccacaggcga gaucaagaga      840

cucuucgaaa uuagcgaguu ugacaaacug cuugauaucu ucuacuccau uaaggaggug      900

aaguacacca cacugcgcaa acagcucaag aucaccgacg cuuucaaauu uaagcaacug      960

gauuaucauc agaauuucga auacaaaacg aacauuaagg ccaagaucga ggacuuuuau     1020

aaacuggaag auuggcucac accggagcag aagguccugc uugaaaaaca agugcuggac     1080

gauaagaaag agaaguucaa augcuacaag uauucuagca uccgguccau ucucagccug     1140

ucugaguccc agaaguuuaa uggggugaaa uacgacacca aggucgaaaa cacaguuuuc     1200

ggaaaacuua auggcuauca cagcaucaag aaagagcuga acaaggaagu guuugaucuc     1260

cuguucauug acaaggauaa auuuaauaag aucgcugaga uucugucuua ccagaaagac     1320

gacaagagcg ccacccagga ucucaaaaag caaguguuca acgacuuuaa uauggauaag     1380

accaucguca acgaagcgau caauucccug cuuaacauuu cuuucagcgg cuuuaauucc     1440

cugagccuca aagccaucaa caagcugcug cccuaccuuu gucaggggcu caaauaucac     1500

gacgcugugg cccauucuga guacaagcac cauucccagu ucaaagcugc caacccucag     1560

caauaucuga gaccccugaa uaaggaggaa aacuaccaga uuacaagccc uacgguuaag     1620

cgcguguucu cucaguuucg gaaaguccuc aaugccguga ucagaaagca cggauccuuc     1680

gaugcuaugc acauugagau ggcgcgcgaa cugaaaaaca gcaagaaaau caaguuugag     1740

auucagaccg gccaaaaaga guaugcccag gaaaaggagg cuauccgggu uaaguuccuu     1800

gaaaucuuua aauacgaggg guccggaaga gaccugcuca agcugcggcu guaugaacag     1860

cagcauggca aaugcauuua cucuaagaaa gagaucgaga uuaauaaacu ucucgaagau     1920

ggguacgugg agaucgacca cauucugccc uggagccgca cauucgauaa cucccugaau     1980

aacaaggccc ucugucuguc ugccgaaaau caaaacaaag cuaaucagac cccuuuugag     2040

uaucuuacaa acagcgacaa gaauagcaaa gauagcaagg aauggcagga guacaaaaac     2100

uaugucucuu ccuucgccgg aaucaagcaa gcgaagaaac auaagcugcu caauaccacg     2160

cugccgccca gacggggcaa cgacuuuagc gaugacauca aaaacccuga gucuggcuuc     2220

cuggcuagaa aucuuaacga cacagccuac gccucccgcu uuauuaagaa uuucgugcag     2280

aaccaccucc aguuuagcca gaaugauacc aucaaacaaa aggucaaggu gcggucuggg     2340

gcucugacca accagcugag auauaauugg ggaauugaag agaaaaaccg cgacaauaac     2400

cuccaucacg ccgaagaugc uaucauucug gccuucgcga cacagggcga gguucagaga     2460

augagcacca ucuccgccaa gcgggaagac uucaaauacc aaacagcuaa ggagcgcaaa     2520

aucaaguuua cgcccccuuu cagcgauuuu aagucugugc uugacgaguc cgucggggau     2580

auuuucguga gcuuugcccc cagacggaaa aucuccggag cugcccacaa ggcgaccauu     2640

aaaccgaagg acacaggcgg gaaauauaag uucccuguua auaacggcau ggccgaaaau     2700

ggagugaucc agcgcgucga uguguuuaaa aacgcuaaga auaaguacca guucauugug     2760

uuuuaccccg ccgacuucua uaaagaugac uuuccugagc uggaucucaa gggcaacgaa     2820

aucgacaaaa acguucaguu ccuguucucu cuguuuaagg acgaucuuau cgaguucaaa     2880

accaagaaga caaaaaccaa ggacagcaaa caucuccugg cuuacuuuaa guauauuaga     2940

uccgauggga aaaucgccuu ccaacagaau uacaaggccg agauugaacg gaaggagucu     3000

gacaacaaaa agggauauaa agauauccug cucgcuacgg gcagcgggcu ggcgucccuu     3060

aagaaauacc aggucucugu gcugggagac auugccgaag ugaagaauga gaagcgccuc     3120

ccccugauca aacaaaugua uaagagcaaa cccaagaaaa aguga                     3165


<210>  3804
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3804
augaaccaga aguucauccu gggccucgac auugggauca ccagcguggg auacggccug       60

auugauuaug agacaaagaa uaucauugac gccgggguca gacuuuuucc cgaagcuaac      120

guugagaaua acgaaggacg gcgcuccaag agaggcucuc ggcgccugaa aagacggcgc      180

auccacagac ucgagcgggu gaagaaacug cuugaagauu acaaucugcu cgaccaaagc      240

cagaucccuc aguccacgaa cccguaugcc auucgcguga aggggcuguc ugaggcgcug      300

agcaaggaug agcucgucau cgcucugcuu cauauugcca aaagacgggg aauccacaag      360

auugacguga ucgauuccaa ugacgauguu ggcaacgaac ugucuaccaa agagcagcuc      420

aauaagaaca gcaaacugcu uaaggacaag uucgugugcc aaauucagcu ggaacgcaug      480

aaugagggcc aggucagagg ggagaaaaac cgguuuaaga cagccgauau caucaaagaa      540

auuauccagc uccugaaugu gcaaaagaac uuccaucagc uggacgagaa uuuuauuaac      600

aaauacaucg aacucguuga gaugcgcaga gaauauuucg agggacccgg caaggggucc      660

ccuuacggau gggagggcga ucccaaggcu ugguaugaaa cccugauggg gcacuguaca      720

uacuuuccug acgagcuucg gagcgugaag uacgccuauu cugcugaccu guucaacgcc      780

cucaaugauc ugaacaaucu ugucauucag agagacggac uguccaagcu cgaauaccau      840

gagaaauauc acaucaucga aaacguguuu aagcagaaaa agaaacccac ccugaagcaa      900

auugcgaaug agaucaacgu gaauccugag gauauuaagg gcuaccgcau cacaaaaagc      960

ggcaagccgc aguucaccga auuuaaacug uaucacgacc ucaagucugu ccuguucgau     1020

caguccauuc uugagaacga agacguucug gaucaaaucg ccgagauccu cacgauuuac     1080

caggacaaag auagcaucaa gucuaagcug accgaacuug acauucugcu caaugaggag     1140

gauaaagaaa acaucgcuca gcugacaggg uauaccggaa cacaucggcu gucccucaag     1200

ugcauuagac uggugcuuga ggaacagugg uacagcuccc gcaaucaaau ggagaucuuu     1260

acccaccuga acauuaaacc caagaaaauc aaucucacgg ccgcuaacaa gaucccuaag     1320

gccaugauug acgaguucau ccugucuccc guggucaaac ggacauucgg ccaggcgauu     1380

aaucugauca acaagauuau cgaaaaauac ggggugccug aggacaucau uaucgaacuu     1440

gccagagaga acaauagcaa ggauaaacag aaguuuauua acgaaaugca gaaaaagaau     1500

gagaacaccc gcaagcggau caaugagauu aucggaaaau auggcaacca aaaugcuaag     1560

agacucguug agaaaauccg ccugcaugac gaacaggaag ggaagugucu guacucccuc     1620

gagagcauuc cccuggaaga ucuucugaac aauccgaacc acuaugaggu ggaccacauc     1680

auuccucggu cugucuccuu cgauaauagc uaccauaaca aagugcucgu uaagcagucu     1740

gagaauagca agaaauccaa ccugacaccc uaucaguacu uuaauucugg aaagagcaaa     1800

cuguccuaua accaauucaa gcagcacauc cuuaaccuca gcaaaucuca ggacagaauu     1860

uccaagaaga aaaaggaaua ccugcuggag gaacgcgaua ucaauaaauu ugaggugcag     1920

aaggaguuca ucaaccggaa ucucgucgac accagauacg ccacccggga acugacaaac     1980

uaucuuaagg cuuacuuuag cgccaauaac augaauguga aggucaagac gauuaacggc     2040

ucuuucaccg auuaucugcg caaagugugg aaguuuaaaa aggagagaaa ucauggcuac     2100

aaacaccaug ccgaagacgc ucucaucauu gcgaacgccg auuuccuguu uaaggagaau     2160

aaaaagcuga aggcuguuaa cuccgugcuu gaaaaaccug agaucgagag caagcaacuc     2220

gacauucagg ucgauuccga agacaauuau ucugagaugu ucaucauucc caaacaggug     2280

caagacauca aggauuuucg gaacuucaaa uacagccaca gaguugacaa gaagcccaau     2340

cgccagcuga ucaacgauac acuguauucc acccggaaaa aggacaacuc uacauacauu     2400

gugcagacca ucaaagauau uuacgccaag gacaauacga cacucaaaaa gcaguucgau     2460

aagagcccug aaaaauuucu gauguaucaa cacgacccga gaaccuucga gaaacuugaa     2520

gucaucauga aacaguacgc caacgagaag aauccccugg cuaaauauca ugaggaaacc     2580

ggggaguacc ucacaaagua uuccaagaaa aacaauggac cuauugugaa aagccugaaa     2640

uacaucggca acaagcuggg gucucaccuu gaugugaccc aucaguuuaa auccagcaca     2700

aagaagcucg uuaaacuguc uaucaagccc uaucgcuucg acgucuaccu gacggauaaa     2760

ggauacaagu uuauuaccau cuccuaucuc gacgugcuga aaaaggauaa uuacuauuac     2820

auuccugaac agaaauauga caaacuuaag cugggcaaag ccaucgacaa gaacgcgaaa     2880

uucauugcua gcuuuuacaa gaaugaucuc aucaaacugg acggggagau cuauaagauu     2940

aucggcguga acucugauac aagaaauaug auugaacugg accuucccga uauccgguac     3000

aaggaguacu gcgagcugaa caauauuaaa ggagaaccgc gcaucaagaa aaccauuggc     3060

aagaaaguca acagcaucga gaagcucaca accgacgugc uggggaaugu uuucacgaac     3120

acccaauaua caaagccuca gcuccuguuu aaaagaggaa auuga                     3165


<210>  3805
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3805
augaagcuga caccccucaa cuacauccug ggccuugacc uggggauugc cagcguggga       60

ugggcugucg uugagaucga ugaacaggag aauccucucg gccugauuga cgugggggug      120

agaacauucg aucgggccga agucccgaaa acgggagagu cccuugcgcu ggcucgcaga      180

cucgcccggu cugcccgcag acuggugaag cggcgcgcug acagaaucaa aaaggccaaa      240

cggcugcuca aggcugaaaa cauucugcuu agcgccgaug agcaccuccc caaugacguu      300

uggcaacugc gcgugaaggg ccuggaucag aaacuugaga gacaggaaug ggcggccguc      360

cugcuccauc ugcugaagca ccggggguau cucucccagc gcaaaaacga gucuaagagc      420

gaaaauaaag agcugggagc ccuucugucc ggcguggaga ccaaccauca aauccuccag      480

ucugcugaau acagaacccc ugcugagauc gccguuaaga aguuucacgu ggaagacggc      540

cauauucgga aucagcgcgg ggcguauacc cacacauuca gcagacugga ucuucuggcc      600

gagauggaac uccuguuuca gcggcaaacc gaccugggaa acccccacac auccgcuaaa      660

cuccuggaga aucuuaccgc ccugcucaug uggcagaagc cugcucuggc cggcgaggcc      720

auccuuaaaa ugcuggggaa gugcacguuc gaacccaccg aguacaaagc ugcgaagaac      780

agcuauucug ccgaaagauu ugucuggcuc acaaaacuga auaaccugcg cauucucgag      840

cagggagcug aacgggcccu gaccgauaau gagagauucg cccuucugga ccagccuuac      900

gagaaggcua aguuuacaua ugcccaagcg cgcaccaugc ucgcucugcc ggaugaagcc      960

aucuucaaag gcgugcggua ccagggggag gacaagaaag ccguggaaac gaagacaauu     1020

cugauggaga ugaaggcuua ccaucagauc agaaaagccc uugaaaacgc ugaccucaag     1080

gccgagugga augagcugaa aaacaacucc gaacugcucg augacauugg aaccgcguuu     1140

agccugcaca agacagauga ggacaucugu cgcuaucuug auggcaaacu gucugaaucc     1200

auccucaaug cccugcugga gaaccuuaau uucgacaagu uuauucaacu cagccugaaa     1260

gcucugcagc agauccuccc ccugaugcuu cagggccaac gguacgauga ggccgucucu     1320

gcuauuuaug gggaccauua cggaaagaag uccgccgaaa ucaacagacu gcucccuacc     1380

auucccgcgg augagauccg caauccuguu gugcugcgga cccugacaca ggccagaaaa     1440

gugaucaacg cugucgugcg ccuuuauggc agccccgccc ggauucacau cgaaacgggg     1500

agagagguug gaaaguccua ccaggaccgg aaaaagcucg aaaaacagca agaggauaau     1560

cgcaagcaga gagagucugc ugugaagaaa uucaaggaau auuuuccgaa cuucgucggc     1620

gagccuaaag ggaaggacau ucugaaaaug cggcuguacg aacuccagca ggccaagugc     1680

cuguacagcg gaaaaucccu ugagcugcac agacuccugg aaaagggcua uguggagguu     1740

gaccaugccc ugcccuucag ccgcaccugg gaugacucuu uuaauaacaa ggugcuuguc     1800

cucgcuaaug agaaccaaaa uaaaggcaac cugacaccuu acgaauggcu ggaugggaag     1860

aauaacuccg agcacuggca gaacuucgug gcgcgggucc agaccagcgg auuuucucau     1920

accaaaaagc agagaauccu cagccacaaa cuggacgaaa agggcuucau ugagcgcaau     1980

cuuaacgaua caagauaugu ggcccgguuu cuguguaauu ucaucgcuga caacaugcuc     2040

cugacgggga agggaaaacg caagguuuuu gccuccaaug gccaaaucac agcccugcuu     2100

agagggcggu ggggccugca gaaagugcgc gaugacaacg auagacauca cgcucucgac     2160

gccgucgugg uugcgugcuc uaccgugguc augcagcaaa agauuacccg guucgugcgc     2220

uacgaagagg gaaauguguu uagcggcgag agaaucgauc gggaaacagg ggagauuauc     2280

ccccugcacu uccccucccc uugggcuuuc uuccgcgaaa acguugagau uagaaucuuu     2340

agcgagaauc cgaaacucga acuggagaac cggcuucccg acuauccuca guacaaucau     2400

gaauuugucc agccccuguu cgugucuaga augccuaccc gcaagaugac aggacagggc     2460

cacauggaga cggugaaguc cgccaaacgg cucgaugaag ggcugagcgu ccugaaggug     2520

ccccuuaccc aacugaaacu cucugaccug gagagaaugg uuaaccgcga gcgggaagug     2580

acacucuaug agucccugaa ggccagacuu gaacaguuug gaaaugaccc ggcuaaagcc     2640

uucgcggagc cuuuucauaa gaagggcggg gcugucguga aagccguucg cguggaacag     2700

acccaaaaga gcggcguccu ggugcgggau ggaaacggcg uugccgacaa ugcuuccaug     2760

gugagagucg auguguucac aaaaggggga aaguacuuuc ucgugcccau uuacaccugg     2820

caggucgcca aaggcauccu gccuaacaag gcuguuacgg ccaacgugga cgagaucgau     2880

uggcuggaga uggacgaauc uuaucaguuc auuuuuacca uguaccccaa ugaucuugug     2940

aaagucaagc ugaagaaaga ggaguucuuu ggguauuacg gaggccucga ccgcgcgaca     3000

ggggccaucg ugauuaagga acacgaucug gagaaaagca agggcaaaca gggaaucuau     3060

cggauuggcg uuaagcucgc ucuguccuuc gaaaaguacc agguggacga gcuugggaaa     3120

aacaucagac cuugucggcc caccaagcgc caacacguca gauga                     3165


<210>  3806
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3806
augaaggaga aauacauccu gggccucgac cuggggauua ccagcguggg auauggcauc       60

auuaacuucg aaacaaagaa aaucauugau gccgggguga gacuuuuucc cgaggcuaau      120

guugacaaca augaaggacg gcgcuccaag agaggcucuc ggcgccugaa aagacggcgc      180

auccacagac ucgagcgggu gaagcugcuu cugacggaau acgaucucau caacaaggag      240

cagauuccua ccagcaauaa cccguaucaa auccgcguga aagggcuguc cgagauucug      300

ucuaaggacg aacucgccau cgcgcugcuu caucuggcua aaagacgggg cauucacaau      360

aucaacguca gcuccgagga ugaagacgcc ucuaaugagc ucagcacaaa ggagcagauu      420

aaccgcaaua acaaacugcu uaaggauaag uacgugugcg aaguucagcu gcagagacuc      480

aaagagggac aaauccgggg cgaaaagaau cgcuucaaaa ccacagacau ccugaaggag      540

auugaucagc ugcucaaagu gcagaaggac uaucauaacc uggauaucga cuuuauuaau      600

caguacaaag aaaucgucga gaccagacgg gaguauuucg aagggcccgg acaaggcucc      660

ccuuuugggu ggaacggaga ucuuaagaag ugguacgaga ugcugauggg ccacuguaca      720

uacuuccccc aggaacucag aagcgugaaa uaugccuacu cugcugaccu guuuaacgcc      780

cuuaaugacc ugaacaaucu cauuauccag cgcgauaacu ccgagaagcu ggaauaucau      840

gagaaauacc acaucauuga gaauguuuuc aagcagaaaa agaagccuac ccugaaacaa      900

aucgcuaagg aaauuggggu gaaccccgag gacaucaaag gauaucggau uaccaagagc      960

ggcacgccuc aguuuacaga guucaaacuc uaccacgauc ugaagucuau cgucuuugac     1020

aaguccaucc uugaaaauga agccauucug gaucagaucg cggagauucu caccaucuau     1080

caagacgagc agagcauuaa agaggaacug aacaaacuuc cggaaauccu gaaugagcag     1140

gauaaagccg agauugcuaa gcucaucggc uacaacggga cacauagacu gucucugaaa     1200

ugcauccacc ucauuaauga ggaacugugg cagaccuccc gcaaccaaau ggaaaucuuc     1260

aauuaccuua acauuaagcc caauaaggug gaccugagcg agcagaacaa aaucccuaag     1320

gauaugguga acgacuuuau ucucuccccc gucguuaaac ggacguucau ccagucuauc     1380

aaugugauua acaaggugau cgaaaaauau ggcauuccug aggauaucau uaucgagcug     1440

gccagagaaa auaacagcga cgaccgcaag aaauucauca auaaccugca gaagaagaau     1500

gaggcuacac ggaaaagaau uaacgaaauc auuggacaaa ccgggaauca gaacgccaag     1560

cgcaucgucg agaaaauucg gcuucaugau cagcaggaag gaaagugucu cuacucccug     1620

gagagcaucg cgcugaugga ccuccugaau aacccccaaa auuaugaggu ggaucacauc     1680

auuccgagau cuguugccuu ugacaacucc auccacaaua aagugcuugu caagcagauu     1740

gaaaacagca agaaaggcaa ccgcacaccu uaccaguauc ucaauucuag cgaugcuaag     1800

cuguccuaca accaguucaa acaacauauc cugaaucugu cuaagagcaa agaccggauu     1860

uccaagaaga aaaaggauua ucuucucgag gaaagagaca ucaacaaauu ugaggugcag     1920

aaggaguuca uuaaucggaa ccugguugau acccgcuacg ccaccagaga acugacaagc     1980

uaccucaagg cuuauuuuuc ugccaauaac auggacguga aggucaagac gaucaauggg     2040

uccuucacca accaucugcg gaaagugugg agauuugaua aguaccgcaa ucacggauau     2100

aaacaccaug ccgaggacgc ucuuaucauu gcgaacgccg auuuccuguu uaaggaaaau     2160

aaaaagcucc agaacacaaa uaaaauccug gagaagccca ccauugaaaa caacacaaag     2220

aaagucaccg uggagaagga ggaagacuac aauaacguuu ucgagacgcc uaaacuggug     2280

gaagacauca agcaauaucg ggauuacaaa uuuagccaca gagucgacaa gaagcccaau     2340

cgccagcuua uuaacgauac acucuauucu accagaauga aagacgagca cgauuacauc     2400

gugcagacca ucacagacau uuacggcaag gauaauacca accugaaaaa gcaguucaau     2460

aaaaaccccg aaaaguuucu gauguaucaa aaugacccua agacauucga gaaacucucc     2520

aucauuauga agcaguacag cgaugagaaa aacccgcugg cuaaguauua cgaagagacg     2580

ggcgaauauc uuaccaaaua cuccaagaag aauaacgggc ccaucguuaa aaagauuaaa     2640

cugcucggaa auaagguggg caaccaucug gacgucacaa auaaauauga gaacucuacc     2700

aagaagcugg ugaaacuuag caucaagaau uaccgguucg auguguaccu cacagaaaaa     2760

ggguauaagu uuguuaccau cgccuaccug aacgucuuca aaaaggacaa cuauuacuau     2820

auuccuaaag auaaguacca ggagcugaag gagaaaaaga aaaucaagga cacggaccag     2880

uuuauugccu ccuucuauaa aaaugaucuc aucaagcuga acggagaccu uuacaagauu     2940

aucggcguca auucugauga ccgcaacauu aucgaacugg auuacuauga caucaaauac     3000

aaggauuauu gcgagauuaa uaacaucaaa ggggaaccca gaauuaagaa aaccaucggc     3060

aagaagacag agagcauuga aaaauuuacc acagacgugc ucggaaaucu guaccugcac     3120

uccaccgaga aggcuccuca acuuaucuuc aaacggggcc uguga                     3165


<210>  3807
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3807
auggagaaca agaaucugaa cuacauccuc ggccuggacc uugggauugc cagcguggga       60

ugggcugucg uugaaaucga ugagaaagaa aauccccuga gacucauuga cgugggcgug      120

cggaccuucg agcgcgccga agucccuaag acaggggagu cccuggcgcu uucuagacgg      180

cuggcucgca gcgccagacg gcucacgcag cgcagagugg cccggcugaa aaaggcuaaa      240

cgccugcuca aguccgagaa cauccugcuu ucuaccgaug aaagacugcc gcaccaaguu      300

uggcagcucc ggguggaggg acuggaccau aaacuugaac gccaggagug ggccgcuguc      360

cugcuccacc ugauuaagca uagaggcuau cugagccagc ggaagaauga guccaaaucu      420

gaaaacaagg agcucggggc ccugcuuagc ggaguggaua auaaccacaa acugcuccaa      480

caggcgacau accgcucccc cgccgaacug gcuguuaaga aguuugaggu ggaagagggc      540

cauaucagaa aucagcaggg cgccuauacc cacacauuca gccggcuuga ccugcucgcu      600

gagauggaac ugcuguuuuc uagacaacag cacuucggga acccuuuugc cuccgagaaa      660

cuccuggaaa aucuuaccgc gcugcucaug uggcagaagc ccgcccugag cggagaggcu      720

auccuuaaaa ugcugggcaa gugcacauuc gaagaugagu acaaagccgc uaagaacacc      780

uauucugccg agcgcuuugu cuggauuacg aaacucaaua accugcggau ccaggaaaau      840

gggcuggaga gagcccucaa cgacaacgaa cgccuggcuc uuauggagca accuuacgau      900

aagaaucggc uguucuauuc ccaggugaga agcauucuca agcugucuga cgaagcgauc      960

uuuaaaggac ugcgcuacuc cggcgaggau aagaaagcca uugagaccaa ggcugugcuu     1020

auggaaauga aagccuacca ucagauccgg aagguccucg aggggaacaa ucugaaggcc     1080

gaaugggcug agcugaaagc caaccccaca cuccuggacg agauuggaac cgcguucagc     1140

cuuuauaaga cagaugaaga caucuccgcu uaccuggccg gcaaacucuc ucaaccuguu     1200

cugaaugccc ugcuugagaa ccucagcuuu gacaaguuca uccagcuguc ccugaaagcu     1260

cucuauaagc ugcuuccgcu gaugcagcag ggccucagau acgaugaagc cugucgcgag     1320

auuuaugggg accacuacgg aaagaaaacc gaagagaauc aucacuuucu gccccaaauc     1380

ccugcugaug agauucggaa ccccguggug cugagaacgc uuacacaggc ccgcaagguc     1440

aucaauggcg ugguucggcu cuaugggagc ccugcgcgga uucacaucga aaccggaaga     1500

gaggugggca aaucuuacaa ggaccgcaga gaacuggaga aacggcagga agagaacaga     1560

aagcagcgcg agaaugccau caaggaguuc aaagaauacu ucccccauuu ugcuggggaa     1620

ccgaaggcca aagauauucu gaagaugcgg cucuauaaac aacagaacgc uaagugccug     1680

uacuccggaa aaccuaucga gcuucacaga cugcucgaaa agggcuaugu cgagguggac     1740

caugcccugc ccuucagccg cacaugggau gacucuuuua auaacaaggu ucuggugcuu     1800

gcgaaugaga accagaacaa aggcaaucuc accccuuucg aauggcugga ugggaagcac     1860

aacagcgaga gauggcgggc cuuuaaagcu cuggucgaaa ccuccgccuu ccccuacgcu     1920

aagaaacagc gcauucucuc ucaaaagcug gacgagaagg gauuuaucga gagaaaucuu     1980

aacgauacac gguauguggc ccgcuuccug uguaauuuua uugccgacaa caugcaucuc     2040

acgggcgaag ggaaaagaaa ggucuucgcu agcaauggac agaucaccgc gcugcugcgg     2100

ucccgcuggg gccuugccaa aagcagagag gacaacgauc ggcaccacgc ucuggacgcc     2160

gugguugugg ccugcucuac agucgcuaug cagcagaaga ucacccgcuu ugugagauuc     2220

gaagccgggg auguuuuuac aggcgagcgg auugacagag aaaccggaga gaucauuccc     2280

cuccauuucc cuacgccgug gcaauucuuu aaacaggagg uggaaauccg cauuuucucc     2340

gauaauccca agcuggagcu cgaaaaccgg cugccugaca gaccccaggc gaaucacgag     2400

uuuguccaac cucuuuucgu gagccgcaug cccacacgga agaugaccgg ccaggggcau     2460

auggaaaccg ugaaaucugc uaagagacug aacgagggaa ucuccguuau uaaaaugccg     2520

cucacaaagc ugaaacugaa ggaucuugag cugaugguca aucgcgaacg ggagaaggac     2580

cucuacgaua cccugaaagc cagacucgaa gccuuuaacg acgauccugc uaaggccuuc     2640

gcggagcccu uuaucaaaaa gggcggggcu aucgugaaaa gcgugcgcgu cgaacagauu     2700

cagaaguccg gagugcuggu ucgggagggc aauggggugg ccgacaacgc cucuaugguc     2760

agaguggaug uuuucacaaa aggcggaaag uauuuucuug ugccuaucua cacguggcaa     2820

gucgcuaagg gcauucugcc caacaaagcc gcuacccagu acaaggacga ggaagacugg     2880

gaggugaugg auaauagcgc cacauucaaa uuuucccucc acccuaacga ccugguuaag     2940

cuggugacca aaaagaagac aauccuuggg uauuucaaug gacugaaccg ggcgaccggc     3000

aauauugaua ucaaagaaca cgaccucgau aagucuaaag ggaagcaggg aaucuuugag     3060

ggcgucggga uuaaacuggc ccucagcuuc gagaaguacc agguggacga acugggcaag     3120

aacauccgcc uuuguaaacc cuccaagaga caaccggugc gguga                     3165


<210>  3808
<211>  3168
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3808
augaacaauu acauccuggg ccucgacauu gggaucacca gcgugggaua uggcauuguc       60

gauuccgaca caagagagau caaggaugcc gggguucggc uguuccccga agcuaacgug      120

gacaauaacg agggacgcag aucuaaacgg ggcgcccgca gacuuaagcg gcgccggauu      180

cacagacugg aucgcgugaa acaucuccug gcggaauaca aucuucugga ccucacgaac      240

aucccuaaga gcaccaaucc guaucagauc agagucaaag ggcugaacga gaagcugucc      300

aaggaugaac ucgugauugc ucugcuucac aucgccaaac ggcgcggcau ucauaauguu      360

aacgugauga uggacgauaa ugacucugga aacgagcuga gcacaaagga ucaacucaaa      420

aagaaugcca aagcucuguc cgacaaguac gucugcgagc uucagcugga aagauuugag      480

caggauuaua aggugcgggg cgaaaagaac cgcuucaaga ccgaggacuu uguuagagag      540

gcccggaaac uccuggaaac acagucuaag uucuuugaga ucgaccaaac cuucauuaug      600

agauacaucg aucuggugga aacacgccgg gaguauuuug aagggcccgg aaaaggcagc      660

ccuuucgggu gggagggaaa uauuaagaaa ugguuugagc agaugauggg ccacuguacc      720

uacuuccccg aagaacucag auccgucaag uacgcuuaua gcgccgaacu guuuaacgcg      780

cuuaacgacc ugaauaaccu cgugaucacg cgcgaugagg aagccaagcu gaauuacggg      840

gagaaauucc agaucauuga gaacguguuu aagcagaaaa agaccccuaa ucuuaaacaa      900

aucgcuaagg aaauuggagu cucugagaca gacaucaagg gcuaucgggu uaacaaaucc      960

ggcaagcccg aguucaccca guucaaacug uaccaugauc ucaagaauau uuuugaggac     1020

agcaaauauc ugaacgaugu gcagcugaug gacaauaucg ccgaaaucau uacaaucuac     1080

caggauccug agucuauuau caaggagcuc aaccugcuuc cggaacugcu cuccgagaag     1140

gaaaaagaga agauuagcgc ucugucuggg uaugccggaa cccacagacu uucccugaaa     1200

ugcaucaauc uccugcugga cgaucucugg gagagcuccc ugaaccaaau ggaacuuuuc     1260

acgaagcuga aucucaaacc caagaagauc gaccugucuc agcagcacaa aauuccuauc     1320

aagcuggugg acgauuuuau ucuuagcccc gucgugaaac gcgcguucau ccaauccauu     1380

cagguuguga acgccaucau ugacaaguac ggccucccug aggauaucau cauugaacug     1440

gcucgggaga auaacagcga cgauagacgc aaauuucuga accagcucca gaagcaaaau     1500

gccgaaacac ggaaacaggu cgagaaggug cugagagagu acgggaacga caaugcuaag     1560

cgcaucguuc agaaaauuaa acuucauaac augcaggaag gaaaaugucu guauucucuc     1620

aaggauaucc cccuggagga ccugcuuaaa aauccgaacc acuacgaagu ggaucauauu     1680

aucccucggu ccgucgccuu cgacaauagc augcacaaca aggugcucgu cagagccgag     1740

gaaaauucua agaaaggcaa ccggaccccc uaucaauacc ugaauagcuc cgagucuagc     1800

cuguccuaua acgaguuuaa gcagcacauc cucaaucuga gcaaaacaaa ggaucgcauu     1860

accaaaaaga agagagaaua ccuucuggag gaacgggaca ucaacaaaua ugaugugcag     1920

aaggaguuca uuaauagaaa ccucguugac acccgcuacg cuacacggga acugacgucu     1980

cugcuuaaag cguacuuuuc cgccaacaau cucgacguga aggucaaaac caucaacggg     2040

agcuucacaa auuaucugag aaaggugugg aaguuugaua aagaccgcaa caagggauac     2100

aaacaucacg cugaggaugc ccugauuauc gccaaugcug acuuccucuu uaagcauaac     2160

aaaaagcuga gaaauaucaa caaaguucuu gaugccccuu cuaaggagau ugacaagaaa     2220

cgggugaccg uccaguccga agaugaguau aaucaaaugu ucgaagacac acagaaggcg     2280

caggcuauca aaaaguucga gauucgcaaa uuuagccaca gaguggauaa gaagcccaac     2340

cggcagcuga ucaaagacac ccucuacucc acgcgcaaua uugauggcau cgaguaugug     2400

guugaaucua ucaaggacau uuacagcguc aacaaugaua aagugaagac aaaauucaag     2460

aaggaccccc auagacugcu gauguaucgg aacgacccuc aaaccuuuga gaaauucgaa     2520

aagguguuua aacaguacga guccgaaaag aauccguucg ccaaauacua ugaggagacc     2580

ggcgaaaaga uccgcaaguu uucuaaaaca gggcagggac ccuacauuaa caagaucaaa     2640

uaucuuagag agcggcucgg ccgccacugc gaugucacca acaaguacau uaauagcaga     2700

aacaaaaucg ugcaacugaa gauuuauucc uaccgguucg acaucuauca guacgggaau     2760

aacuacaaga ugaucacaau uagcuauauc gaucuggaac agaaaucuaa uuacuauuac     2820

auuuccagag agaaguauga acagaaaaag aaagacaagc aaaucgauga cagcuacaaa     2880

uuuauuggau cuuucuauaa gaacgauauc aucaauuaca acggcgagau guaccgcguu     2940

auugggguga augacuccga gaagaacaaa auccagcucg auaugauuga caucagcauu     3000

aaggauuaua uggaacugaa uaacaucaaa aagacgggcg ucaucuacaa aaccauugga     3060

aagucuacaa cccacaucga gaaguauaca accgacauuc uuggcaaucu guacaaagcc     3120

gcuccuccca agaaaccuca gcucaucuuu aagcggggga uggaauga                  3168


<210>  3809
<211>  3168
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3809
augaccccca ucagcgaguc ccuucucuuc ggccuggacg uggggauuuc uagcugcgga       60

ugggccgucc uuaagcacuc cgauacaacg ggccauaucg aagcucuggg gucuuggugu      120

uuuaacgccc cugagaccaa aacagaaaga accccgacaa aucagauucg gcgcagcucc      180

agacuccugc ggcgcguugu gagacggcgc agaaaccgga ugaccgagau ccgcagacuu      240

uucgcgcggc acggacugau ugacaaugau cgccccgacg cucucagaca accugccauc      300

gaucccuggg aaaugcgggg ccaggggcug gaccgccagc ugacaccuga ggaguuugcc      360

guggcucucg cccauaucgc uaagcacaga ggauucaaau cuagcgccaa gcgggucaaa      420

uccaacgcga gccccgauga cucuaagaug cuggccgcuc uugaaaagac caaagagcug      480

uccgcccagu acagaacgau uggcgaaaug uuugcucgcg auagcgccua ugugcaacgg      540

agacgcaauc gggacggccu cuucgauaga accaucucuc gcgacgaucu gauugacgag      600

gcgaagaucc uuuuugaccg gcagagaaaa cuggggaacc cuguugccuc cacagagauu      660

gaagaggcuu ucacccgcau cgccuuuugg cagaauccga ugcaggauag cggagugcuc      720

gucgcuucuu gccccuucga accuaaggag aaacggucca gcagauuuuc ccccucuuuc      780

gaacgcuuuc ggcugcugug uagacucauu aaccugcggg ugacagacgg cccugaggag      840

cgcccccuua ccgaagagga acugagacuc gccuacgagc augccgggaa gacgcggaag      900

cugacagcug cggccaucag aaaacuuauc ggacugcgcg augaccaaca cuucaccaca      960

auuccgccug aacaggagaa gcaggauauc accagccgga ccggcgagac auucgcuggg     1020

acggccaccu uuagauccgu ucucggagaa agccuguggc gccagcugcu cccccacccu     1080

gaguggcugg acaaugccgc uuggauuauc ucuuuccaug aacuuacaga gaccauuauc     1140

aaaaagcugg gcgaaaucgg ccuccccgug gaggccuaug agcugauuug cgaugcgcuu     1200

aaauccgaca agagccccuu ugcuaaauuc aagggggccu cuagccuguc cgauaaggcc     1260

gucagagcuc ucaucccuua ccugcaacag ggacugacau augacaaagc cugucagaag     1320

gcuggcuacg aucacgccgc gucucgguuu agcgggcgcg ccaccauuga cacgaaagcu     1380

aaguucaacg cccucauggc ugaagugaga gacuccaucg ccaauccgau ugcgcggaaa     1440

gcccugagcg agggaaugaa gcaacuuugg gcuaugcgca acagaugggg ccugcccggg     1500

gccaucugca ucgaacucgc ucgggaugug ggaaauucuc uggagaagcg caaagagauu     1560

gaaagaaacc ugaagacaac cgccaaacag cgggcccgcg agagagaaga ggcuugugaa     1620

cuucucaauc ugaaggaggu cuccagcgac auccugcucc gguauagacu guggaaagag     1680

cagggcggcc gcugcuguua cucugggaag uccauuaccc cugauaugcu uauggcgcag     1740

gacaaccgga uccaaauuga ucauauccug cccuggagca gauuuggaga cgauuccuac     1800

ucuaacaagg gccucugccu ggccaaagaa aaucaggcua agaaaaacca gacaccuuau     1860

gaguggauuc acgggcaggg aaccacagcc caaaaggaac agugggacaa auucguugcc     1920

gauguggaga gccgcaagaa uacgaagggc cugaaaaagc ggaacuuucu ucucaaaggg     1980

uccaaggaaa ucgaggagag auuccgcucu cggaaucuga acgacaccag auacgcugcc     2040

cgccugaugg uggaagcggc ucggcuccug uaucccgccg gccagagagg agagaaaggc     2100

gggacacggc gcgucuuuac cagaccggga gcccuuacag cugcccugcg gcacgcgugg     2160

ggcguggaaa gccucaagaa aaucgauggg gagcgccugc augacaauag acaccaugcu     2220

cuggaugccg ccauuguugc ugccgugucc gaagcugaga uccagaagcu gaccaagagc     2280

uuccaagagu gugaacagaa aggacucucu cggccuauga gagacgucgc gcccccuugg     2340

gacggcuuuc gcgaucagcu gaaggagaaa uacgaaacga uuuucguggc ccggcccgag     2400

agacagcgcg cccgggggca aggccacgag gcuaccauca gacagguucg cgaagaggaa     2460

acaggaccug ugauuuauga gcggaaggcc gucuccgcuc ugacccucgc cgaccuggcc     2520

aaaaucaagg aucccgacag aaacgcuggc cuuaucgcga gccugcgcca guggauugaa     2580

gccgggaagc cggcugauaa accucccaga acacccugcc aucaaucugc cgccggacac     2640

gagauccgga aggugcgccu cgcuaccauu aucacgccug ccgucccggu gagaggcggg     2700

auggcgaaac ggggagagau gacacgcguu gacauuuuca aggugaucgg caaaaagggg     2760

aagcccgaau gguacaugau cccuauuuau ccccaccaga ucaugaauag aucccagugg     2820

ccugcucccc cuaacaaagc cgucguggcc ggcaagagcg aggcugauug gcccauuaug     2880

ggagccgaag cgcaguuuca auucucucug uacccgugca gcuacguucg gcuguuuaaa     2940

gagggcaccg ugaucgaagg guauuucugu ggaugccaua gaucccgcgc uuacauuaca     3000

cuugcccuca gcaccggcgc cgcuccucug cagaagggga ucggaaucaa aacccaggag     3060

cacauucaga aguuuucugu caaucgguuc ggcgagcugu cccccgugaa gcaagaaaca     3120

agaacguggc augggguggc cuguaccagc ccugaccagc acgauuga                  3168


<210>  3810
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3810
augaaggccc ugaacuacau ccucggccug gaccuuggga uugcuagcgu gggaugggcc       60

gucguugaga ucgaucagaa ugaacugccc auuagacuca ucgaccuggg cgugcggacc      120

uucgagcgcg cggaagugcc uaaaacaggg gauucccuug cucuggccag acggcucgcc      180

cgcucuagca gacggcuggu ccgcagacgg gcugugcgcc ugcucaaggc caaaagacug      240

cuuaagcaag aggggauucu gcaggcugac gauuuucacu ccucucaucu caaaccgcug      300

cccaacaauc cuugggaacu ucggguuaag ggccugaacc accuccugga ccgcaaggag      360

ugggccgcgg ugcugcucca ucugcuuaaa cacagagggu aucugagcca gcggaagaau      420

gaguccaaaa cggauaacaa ggaacucgga gcccugcuua aaggcgucuc ugagaaucgc      480

cagcugcucc aagcucaggc cuucagcacc cccgcugaaa ucgccaucaa gaaguuucag      540

caggaggagg gccauauuag aaaccaacgg ggagcguacu cccacacauu cgacagaaau      600

gaucugcugg ccgaacuccg ccugcuuuuu cagaaacagc gggaguucaa caauccuuau      660

accagccagu cucuggaaca agcuaucgcc gagcuccuga uguggcagaa gcccgcucuu      720

uccggagaag ccauucugaa aaugcucggc aagugcacau uugagccuag cgaguacaaa      780

gccccgaaga acucuuauuc cgcugaauac uucguguggc ugaccaaacu gaauaaccuc      840

agaauccugg agaauggggc ggaccgcgga cuuacagccg aggaacggca gcugcucauu      900

aaccaacccu uugaaaaggc uaaguucacc uaugcccagg ccagaaaacu gcuggagcuu      960

agcgaucucg cuaucuuuaa gggcguucgc uacgggaaag acaacgccga gucugcgacg     1020

cugauggaaa ugaaggcuug gcacgccauu cggaaagccc uggaguccaa uggacucaag     1080

accgaaugga acggccugaa gacaaaaccu gagcuucugg auaagaucgg caccgcuuuc     1140

agcaucuaca aaacagacga agacauuucc caguaucuca auggggagcu gucucccgcc     1200

gugcugaacg cucuccucga gaaucugagc uuugauaagu ucaucagacu gucccuccag     1260

agccuggcca aaauucuucc ucugauggaa caaggacucc gcuacgacga ggcguguaag     1320

caggucuaug gcgaucauua cgggaagaaa gaacagcugg agcugcgguu ccuucccaag     1380

aucccggugg aggacauuag aaacccugug guccuccgca cccugucuca ggcccggaaa     1440

guuaucaaug gaguggugag acuguauggc ucccccgcuc ggauccacau ugaaacgggg     1500

cgcgaggucg gaaagagcuu uucugaaaga cgggauauca gcaaaagaca agaggaaaac     1560

cgcaaugagc ggcagagagc cguggagaag uucaaggaau uuuucccuca uuuuguuggc     1620

gagcccaaag cuaaggacau ucucaaaaug cgccuguacg aacagcagca cggcaagugc     1680

cuuuauuccg ggaaagagcu ggaucucaac agacugcugg aaaagggaua cguggagauc     1740

gaccacgccc uucccuucuc ucggacaugg gaugacagcu uuaauaacaa aguccucgug     1800

cuggcguccg agaaucaaaa caagggcaau cagaccccuu acgaauggcu gaacgggaag     1860

gagaacgccg aacgcuggaa acucuuccag gcucugguua auggaagcag auuuccguau     1920

ucuaagaaac agcggauucu uacaaaguac cuggaugagg acggcuuccg cgaaagaaac     1980

cucaaugaca cccgguaugu ggcccgcuuu cuguguaacu ucaucgagga uaauaugcaa     2040

cuggucgggc agggcaaaaa gagaguguuu gcuuccaacg gacagaucac cgcccuucug     2100

cggcgcagcu ggggcauuca aaaggucaga gaggacaaug aucggcauca cgccguggac     2160

gcuguugugg ucgcgugcuc uacaagagcc cuccagaaaa agaucacgga uuacgugcgc     2220

uauaaagaaa ugaacguuuu caccggggag aagauugacc aggaaacagg agagaucauu     2280

aaauuccauu uucccgagcc uuggaaguuc uuucggcagg aaguggagau cagagucuuc     2340

uccgaaaccc ccgcugagga uguggugauu gcccugccug accgccccca agccaaucac     2400

gaauuuaucc agccgcuuuu cguuagccgg gcuccuacaa gaaagaugac cggccagggg     2460

cauauggaga cgaucaaauc cgccaagcgc cuggcggagg gacuuucugu cagccgggug     2520

ccccugacac agcucaaacu gaaggaucug gaaaacaugg ugaauagaga gcgcgaaaaa     2580

gcucuuuacg aggcccugaa gguccggcuc gaaaaguuua acaaugaccc ugccaaagcc     2640

uucgcugauc ccuuuuauaa gaaaggcggg caacagguga aggcgguuag aguggagcag     2700

guccagaaau ccggcgugcu gguucggcaa gagaacggag uggcugacaa ugccucuaug     2760

guccgcgugg auguuuucau uaagggcggg aaauacuuuc ucgugccuau cuacaccugg     2820

caggucgcca agggcauucu gcccaacaga gcugccaccc agggaaagga agaggaauau     2880

ugggacauca uggacgagac agcuauuuuc caauuuagcc uuuaccagaa cgaucugauc     2940

aaacucguga ccaagaaaaa gacaaucuuc ggguauuuca auggacugaa ccgggccacg     3000

uccaauauua acaucaaaga acacgaccug gauaagagca agggcaaaga cgggauuuac     3060

cuugaggugg gcgucaagcu ggcgcucucu cuggagaaau aucagguuga ugaacucgga     3120

aagaauaucc agccgugucg cuccaugaaa aagagaccuc ccgugcggug a              3171


<210>  3811
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3811
augcccaagc agaaaagcac cagauaccgg cuggcccucg accugggcuc cacaucucuu       60

gggugggcug ugcugcgccu caacgagcaa cacgaaccua augccaucau uagagcggga      120

guccggaucu ucagcaacgg ccgcaauccg aaggaugggu ccucucuggc uguuacgaga      180

cgggccgccc gcgcuaugag acggcgcaga gaccggcuuc ugaaacgcaa ggccagaaug      240

augaacaaac ucguggagua uggauuuuuc cccgcugaug uggccgaacg gaagaagcug      300

gagcgcauua auccuuacga acugagagcg aaaggccucg acgcuccccu gaccccuggg      360

gaguuugccc gggcccuuuu ccauaucaac cagagacgcg gauuuaagag caaucggaaa      420

acagauaaga aagagaacga cuccggcgcu cugaaggccg cgauuucuca ccucagacag      480

cagcugcaug aacgcaaggc ccggaccguc ggcgaguggc uuuggcaaag acugcaggcu      540

gggcucggaa cacgcgcccg guauagagaa agccgcgugg cucaggccaa uggcaaaacc      600

cggaucgaga aguccuacga ucuguauguu gacagacaga ugaucgccga ggaguucgau      660

gcucuguggg cggcccaagc ugccuuuaac ccccagcagu ucagcgagca ggcccgcggg      720

gaacuccggg acgugcugcu uuuucaaaga gaucugcggc cuguccgccc gggaagaugc      780

acacuccugc ccgacgagga acgggcuccu cuugcccugc ccucugugca gagauuccgc      840

auucuccagg aggcgaauca ccuucggcug cuccauccug aucugagaga ggaaccccuu      900

ucccuggcuc aacgcgacgc cguuguggac gcccucgagc acaacagcaa aagaaccuuu      960

gaacagcugc ggaagcugcu gaaacucaau ggcacgguca aguucaaccu ggaggaugaa     1020

aaacgcaagg agcugaaggg gaaugcuacc ucugccacac ucuccaaaga cggacuguuu     1080

ggcgagaacu gguggaaccu ugaugaagcu cugcaggacg ccaucgugug gcagcucgug     1140

agcgagcaau cugaagagaa gcugcagaau uggcugcagg cgaacaccgg cgucaaugcc     1200

acacaggcug aaaccauugc caacgcuggg cuuccggagg gauacggcuc ccucagcuau     1260

aaagcccugg cgagaaucau uccugcccug cgggggacgg agguugcuaa uggaaucgau     1320

gugccccgca cauacgacca agccgugcag gcugccggcu ucgcccacca uuccgaucuc     1380

gacucugggu ucgauuuuga acacagccau gacgaggucg aaagaauugg agagcgggug     1440

aucgcuucca ccggcgaggu uaagccugug uucgcguuua aacagcugcc cuacuauggc     1500

aaggcccuuc gcagacacgu cgcuuucgcc aaggaacacc cccggaacga ggaagaacgc     1560

uacgggaaaa ucgccaaucc uacagugcau auuggacuga accagcucag aguugugguc     1620

aaugcucuga uccggagaua uggccgcccg accgaagugg ucguggagcu ggcccgggau     1680

cuuaagcaaa gcagagagca gcgccaggag gaacagaaac ggcaagcgga cuaccagaag     1740

agaaacgaac acauucgccg ggagaucgcu ucuaugcaug ggugugccga agagagaguu     1800

ucccacgccg acgugcagaa auggauucuc ugggaggaac ugagcuuuga ugucgcugac     1860

cgccggugcc ccuauucugg agugcaaauc agcgcccaga ugcugcucuc caaucagguu     1920

gagaucgaac auauucugcc uuucucucag acccuugaua acagccugaa uaacagaaca     1980

guggcgaugc ggcaagcuaa ucgcaucaag ggcaacagaa cgcccuggca ggcccgggag     2040

gacuuugaac gccaggggug guccuacgau agcauucucc agagagccga gcggaugccu     2100

aaggcuaaaa gauaucgcuu cucucgggac ggcuacgaga gauggcugaa ggaagauaaa     2160

gacuuucugg cccgcgcucu uaacgauacc cgguaccucu ccagagucgc caagacauau     2220

auggagcugg uguguccccu gggaacccgc gugaucccgg gccaaaugac agcgcuccug     2280

cgggccaaau ucgggcuuaa ugacguucug ggacucaacg gcgaaaagaa uagagaugac     2340

caccgccacc augcugucga ugccugcgug auugggguga ccgaccaggg acugcugcag     2400

agauuugcuc aggccagcgc cgacgcucgg ggcaacgggc uugagaagcu cgucgagaau     2460

augccugugc ccugggcgac guaccgcgau cacguuaaaa gagccaucca auguaucugg     2520

gugucucaua agccugacca cggcuaugaa ggacccauga uggaggaaac agcuuacggc     2580

auucggaaag auggguccau ccgccagaag accaaagccg acggaagcac cggcagagag     2640

auuacacacc ugauccggau uaccgaaccu acacagaacc ugagacaugg ggucgaugcc     2700

caaggacagc cccucgcuua uaagggcuac guugggggcu ccaaugacug caucgagauu     2760

acgcagaacg agcagggaaa augggaaggc aaugugauca ccacauucca agccuaugau     2820

aucgugcgcc ggcugggguc uaagcaggga ccgagacgcc uucggcaccc uacccuggcg     2880

cagacacucg acgucuacgg caagcccaaa aagcugguga ugagacugau gauugaugac     2940

uguguucggc uugagcugga ugggaacaaa aagacccucc gcguggucaa aaucacggga     3000

aauggccagg uguuuauggc ucccgugcau gaagccaacg ucgacaagag aaauagcgau     3060

aagaccgacc cuuucgccua cguuuccaaa uaugcugggu cuuuccaaaa ggccaaagcg     3120

cggcgcauua caaucagccc gauuggcgag cugcacgacc ccggauuuug a              3171


<210>  3812
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3812
augagcgaca aguccaaaau ccugggcuuc gauauuggga ucaccucuau uggaugggcc       60

uacguggagg gcgaagaacu caucgacugc ggggucagaa uuuuuacaaa ggcugaaaac      120

cccaaaacga aggagagccu ggcccuuccu cggcgcgaag cgagaggagu ucggcgcaga      180

cuggcucggc gcaaaggcag acucaaugcc aucaagcagc ugaucuguaa ggaguucggg      240

cuuaacuauc aagauuacau ugcccaggac ggagagcugc cgaaggcuua ugccggcaaa      300

cucaucaauc ccuacgaacu gcgguccaaa gcucugcgcg agaagcucga aaaagaggag      360

cuggccagag ugauucuuca caucgcgaag caucggggcu augggaacaa gcacgccaaa      420

gaaaccgaug acgcugaguc uggaaagauu aaaaaggcca ucagcaauaa cgagaaaauu      480

cugaaugaga agggcuacca guccgcuggg gaauaucucu accaggaguu uuuccaaaaa      540

gagcgccugc aggaagagaa gaagggaaac aauacaacca aagaauuuaa gaacgugaga      600

aauaaagccg gcgauuacga gcauugcauc gcgcagacac agaugcaaaa cgaacuucgg      660

cugaucuucg agaagcagcg cgaguuuggg cucaauuucu cugaagaguu uaaaaagaag      720

guccuggaaa ccgccuucuu ucagagaccu cugaaagacu ucagcgauaa gauuggagac      780

uguacauuua ucgagggcgc uaaacgggcc cccaaggauu cccucagcgc uauugaguuc      840

guggcccugg gcagaaucau uaacacccuu aaaaaucugc agaacacguc uggggaaguu      900

uauucccgcg acaagaucaa cgagauccuc caaauugugc uggauaaggg agaaaucagc      960

uacaaaaagc ugcgggagau ucuggacauc gaaucuuccg ucagauucaa agacagcaag     1020

cucgauuaua ccaaagagcu gaaggaggcc gaaaagacaa aacuguuuga gucuaagaau     1080

cuccagaaau ucaagaaagc ucugggcucc uuugaaggga uuagccgcga gauccuugac     1140

uccaucgcga aggaaauuau gaucaccaaa gauaaggaga agcuggccaa agagcucaag     1200

aaauucccuc uggacgaaac acagauucaa aaccuuucua aucugagcuu uucccagacc     1260

aucaaccuca gccuggaggc ucugucucag auucuccccu ucaugcgggg agaaagagag     1320

gauaagugcc ugcgcuacga cgaagccgug gagaaagccg gccuuaagga gguugcuaag     1380

aauaaaucca aggaugacuu ucugccuccg cucgaaaacu augagcccua ccuggccaau     1440

ccuguggucg cgcgggcucu gagcgaauau agaaaagugc uuaacgcccu ccugcgcaag     1500

uacgggaaaa ugcacaagau ccauauugag uucacgcggg aagugggaaa gucuaaagag     1560

gagagaaaua agaucgaaaa agagcagaag gaaaacuuug ccaaaaauca agaggcucag     1620

aagcagugug agauccuggg ccuccccauu aacggcgcca auauccugaa gaugaaacuu     1680

uggcgcgaac agcaagagag cugcgucuac uccgggaagg aaauugcuau cgaugaccug     1740

aaaaacccug ccauucucca gguugaucac aucuaucccu acucucggag cuucgacgac     1800

uccuauaaua acaaggugcu gguguuuagc aaagagaacc agaauaagcu gaacaaaaca     1860

ccguucgaag cguuuggaga gaauaaggau aagugggaga aaauccuuuc ucucgccucc     1920

aagcugccua aacccaagag acagcggauu uacaacaugc aauucaaaga caaggaacag     1980

ggcuuccuga cccgcaauau caacgauaca ggguauauug cuagacucgu cagccagugg     2040

accaagaauu gucugucuuu ugagaaacuu uccgaagacg aggugagcau cgccggagaa     2100

aagggcucca aaguucacgu ggaggucauu ucugggagcc ugaccgcuau gcuccggcau     2160

uacuggggcc ugggagauaa gaacagagac aaucaccuuc aucacgccgu ggaugcgauc     2220

aucauugccu auuccaacga gaaaguuauc aaggcuuucu cugacuacaa gaaacaccgc     2280

gaacagaaua aggccaaauu uuacgcuaag gagauugccg aaagcgagua uaaaaagcaa     2340

aagguguucu uugaaccuac aaaccauuuc cggcagaaag uccuugagaa gaucgauucc     2400

auuuuuguga gcaaaccucc cagaaagcgc gccagaggcg cucuccacga ggaaacguuc     2460

uacucuuuug agcggcugug caaugacaaa gaauaugcga agaaggagaa aauggauuac     2520

gggggaaagg ccggcaucca gaaagcuauc gcccugggga agauucgcca agucggaacc     2580

aaaaucgugu ccaacggcac aaugguuaga guggacauuu uccagcauaa gagcaauggg     2640

aaauuuuaug gcguccccau cuacaccaug gauuucgccc ucggagugcu gccgaacaag     2700

gcuguugugu cuggcaagga caaacagggg gucauuaagg acuggcuuga gauggauucc     2760

aacuaugaau uuuguuucag ccuguucaaa gacgaucuca uccuggugca gaagaaagag     2820

auggaaaagg ccgagcugug cuacuacaca gacuuuaauu cuaacaccgc gggaauuaag     2880

uucgaaaaac acaauaaccu uuaugugaau cucaacgaga augagcggaa gcuguuuacg     2940

agcaaagaag uugucaaggg cgggaaaaag aacgugugga aggauaaaac acccaaggag     3000

aucgaacaac uggagaaaaa ucgcaagauc auuggaggcu ucgugaccau cgaagaccuc     3060

aaaaccuuug agaaguacca ggucuccccu cugggggagg ugcagaaggc ugauuucgcc     3120

cccagacagg ccauuacacu uaaaagcucu ccuaagcauc ggaucaacaa auga           3174


<210>  3813
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3813
augagcaaca gagugcccua ccggcuggcc cucgaccugg gcaccaauuc caucgggugg       60

ugccuucugg aucucaacgg aaauggcgac ccuugugggg uccgcagaau gggaguucgg      120

auuuucccgg auggccgcga ccccaagucu aaagagagcc uggcugccga uagacggcuu      180

gcgcgcucca ugagacggcg cagagaccgg uaucugcgca gacggucugc ucucaugaac      240

gcccuggugg ccgcugggcu gaugccugcc gaucgcgcua ccagaaaggc ccucgaaagc      300

gaggaccccu gcgaacugcg ggcgagagcc cuuuuugagc cucugacggc uggacaggug      360

ggccgcgccc ucuuccaccu gaaucaacgg agaggcuuua aauccaaccg caaggcugcc      420

ggggaagaga augaggcggg aaaaaucaag gccggcauuu cugcucuuaa ggccgaaauc      480

gaggcuagcg gggaaaccac acugggagcc uggcucgccg cucggcauag aaaacgcgag      540

accguccggg cgagacugga uggcgccggg gcuaaggccu ccuacgaguu cuaucccgaa      600

cgcggacugg ugcacgacga guuucaggcc auucggacag cucaggcccu ccaucacccu      660

gcgguuaccg cugaacagug ggauagagug gagagcguca ucuuccgcca acggccgcug      720

agacccgugc ggccuggcaa auguacacuu uuucccgacc aggaacgcgc cccuugggcc      780

cugcccguug cucaggaguu cagaauccuc accgagauug gcaaccugaa acuuuaccgg      840

ccgggggaag aaccuagaga acugucugcc gaucagcaug cucaacucuu ugccaagcug      900

ugccgcuccg cggaccugac guucgagaag auccggaagg aacucaaacu ggaccccgga      960

cuuaagguga aucuggaggg cgagaaaaga gaucgccuca agggggacgc cgucaccagc     1020

gcucugucug ccaaaaagag auuuggaccu aaauggaacg cucuuacacc cgaugaccgg     1080

cgcgauauug ugggcagacu gaucgaagcc gaggacgaag cggagcucgc cggguggcug     1140

gaauccgugc acggacugcc cagcgagaau gcugcccaca ccgcugccuc uccuggccuc     1200

ccggauggcu auugucggcu guccgagaag gcccuuguca agcugguucc ccucaugcgc     1260

gaacagagac ugcgguacga cgaggcugug cuggcgcuua accuccauca cagcgauuuc     1320

cgcccugaag ggcugcugga cgagcucccc uauuacggag agccucugga aagauuuauu     1380

gccuucggcu ccggggagcc cucugauccg ccugaacagc aucuuggacg guuccccaau     1440

ccuacagugc acaucggccu gaacgagcuc cgcaaacugg ucaauagaau uguggaacgg     1500

ugggggaagc ccgcugacau cguuguggag cuggccagag accuuaaacg cagccgggau     1560

gagagagaca ccauccagcg cgaacaagcc cagaaccagc ggaagaacga ggauagauuu     1620

gcuaaaauua aggaacucgg caucgacccu cgccccgauu ccaugcugcg gcugagacuc     1680

ugggaggaac ugccgaaggu cggagugggc cacguuugcg uguacacggg ggagccuauu     1740

ggaauccgcc ggcuucugga gggcgacguc gauauugacc auauccuccc cuucagcaga     1800

acacugucug auggggccga caauaaaauc gugugugucc ggcgcgcgaa cagauuuaag     1860

cgggaucgcu cccccuggga ggcuuucgga gugaccgccg uugacggcua ugauugggcc     1920

gcuauugccc aaagagcgga ggaacugccu aaaaauaagc gguggagauu ucuuccggac     1980

gcuauggacc gcuucgaggg ggaacggggc uuucucgaua gacagcuggu ggagacacgc     2040

caccugagcc gggucgccag acaguaccuc gccucuaucg uggaccugaa caaaguucgc     2100

guggcucccg gaaaacuuac cgccaugcug cgggcuagau ggggccucaa uaagauucug     2160

agcgauggga accugaagaa ucgcuccgac cauagacacc augccaucga ugcggccguc     2220

cuuggaauga uugaccagcg gcucgugcaa cugguggcuc aggccgcugc ccgcgccgag     2280

gcuaacgaac uggagagacu cuucgauggc cugccugacc ccuuugaugg gcuugcggcc     2340

accucuuucg gagaccaggu ucgggaaaag auccgcggca ucacagucag ccacagagcu     2400

gagcacgggu ggcagggccg gaugcauaau gauacggccu auggaccugu ggaggccgug     2460

aacggcgucc acaccguggu uacaagaaaa ccccuguccg ggcucaccgc uaaggacuuu     2520

ccuaauauuc gcgaucgggu gcuggccaaa gcgcuggaag accaugucgc ugccgaggcc     2580

gcugccggac gcagcuucgg cgacgcgcuu gggucuuuua aguggggagg ccaacccgug     2640

agacggguua gaaugcugga ugugcucggg aacccgaucc cuauucagga ccccuccggc     2700

acagucuuca agguguacaa aggagauagc aaugcuuggu augaaauccu gcgggacccu     2760

cgcggcagau ggaccgggaa gauuguuucu acguucgagg ccuaccggcu ccaccgcgaa     2820

uggcuggccg agacaggagg cgauuccggg agaggaagcg gcguguuucu ugacugggcu     2880

gccaaacggg ucgugggcga ggcugggaca ccccuguucc gccucuuuaa gggagaucug     2940

cuggcccuug gcgaagcgcc ggacagacgg aucgugcgcg ucguuaaauu cuccgaggga     3000

cagguggccc uggcucucca ccuggaagcc gggaaccuca aggcuagaga ugccgacaaa     3060

ggcgauccuu uuaaguauca guacugcucu cccgacagcc ugcggaagca agccgcuaga     3120

ccugcggggg uggaccuucu gggauacguc caugauaaag gcccccucga guga           3174


<210>  3814
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3814
augagacggc ugggccucga caucgggacc aacagcauug gauggugccu gaucgaggau       60

gacaagaaaa uuguggauau cggcguccgc auuuucuccg acgggagaga ucccaagucu      120

ggagccagcc uugcuguuga ccggcgcgau gccagagcga ugcggcgcag acgggaccgc      180

uaccugggca gacgguccgc uuuucuccag gcccuggugg ugcacgggcu uaugccugcc      240

gaugcugacg aagccaaacu ggucgcugau cgcgacccgu aucaacucag agccaaggcg      300

cugcaugugg cccuggagcc cuucgaaauc ggacgggcuc ucuuucaccu gaaucagcgc      360

agaggcuuca aaucuaaccg gaaggccgau agaaaggcua augccggcga ggacgggaaa      420

aucgcggaag gagccaaggc ucuugaccag gccaugagcg aggcuggcgu ugauacacug      480

gggcaguuuc ucgcccugaa agagacgaag cgcgugcggc uuggauccga cggccaagcc      540

uacgauuucu auccucagag acgccauauu gaauacgagu uugaccggau cuggaaaguc      600

cagucugaau aucaccccgc ucugcucacc gaggcggcca gagcugcccu gcaucgcauu      660

cuguucuuuc agcggccucu caaggcccaa gagguggggu ucuguacauu ugcuggaguu      720

aacggcaucg ccgcgaauga aagacgccug cccaaggcuc acccucuuuu ccagcagcgg      780

agacuguacg aggaagugaa ccagcuccgc auugucagcg ccggcgccgc ugaucggccg      840

cugacccuug acgagagaga ucggcugauc cuccgccugc aagacagaaa gaaggugucc      900

uuugauacac uggccaaaaa ggaccuccgg cuggcugaug gggaaagauu caauaaagag      960

agcgagaacc gcaaggaacu uauuggagac gaggugcggg ccgaaauguc ugacaaaaag     1020

agauucggca cccgcugggu ccacuuuggg cuggaugagc agcucagaau ccuggcgaaa     1080

cuucaggacg aagagaaucc cgaggcccug cucgauuggc ugcgguccga auauggacug     1140

gacgaggcug ccgcugaaag cguugccaac gcgcgccucc cugagggcua cgggagauuc     1200

ggagaaacag ccacccggcu gcuucuggau gcucucaaag ccgaggugau cacguacgac     1260

aaggcugccc ugcgcgccuu uggccaucac ucugauuuca gaaccggcga ggugcuggac     1320

cggcuucccu auuacgggga acuccugaca cgcgagauug cuccuggaaa acccgaauau     1380

ggcgauccgc uggagagaca auacgggaag aucaccaauc cuacagucca uauuggacuc     1440

cggcagcugg agaaacuugu gaacgcgguu aucgccgugc acggccgccc cgaccagauu     1500

gucguggaac uggcuagaga gcucaagcug aaugaaaagg agaaagauga acacaagcgg     1560

agaaucaaac gcgacaccga ugccgccauc gcucggggga gaaagcuggc cuccgagggc     1620

uuuauugaca acggagcgaa ucgcaugcuu augcggaucu gggaggaacu caacccugcu     1680

aacccccugg acagacgcug ccccuauugu gccgagccua uuggcauccg ggcccuguuc     1740

agcggggaag cugauauuga ccauaucauc ccguacucua gaucccucga ugacaauguu     1800

ggaaacaaag uggucgccca ccgcagcugc aaucgggcga agggcaacaa gacgcccuau     1860

gagaaauggg ggccucauau ugaucccgaa agaugggagc ggaucuccga ccagguggcu     1920

cgccugcaca agucuaaaca auggagauuu ggaccugaug ccauggagcg gauugaccgc     1980

gauggcgggu uccuugccag acagcugaca gacacccagu accucagccg ggucgcuggc     2040

aaguaccuga gaucccugua ucccacagcc cagagcggac gcguggaugu uaucccgggc     2100

cggaugaccg cuaugcuuag acgcaaaugg gggcucaauu cucugcugcc ugaccauaac     2160

uacguggaaa augagcacuc caacgccccc aagaaucggc ucgaucacag acaucacgcg     2220

auugacgccg cugucgccgc ugugaccaca cugagccuua ugcaacagau cgccgacgcc     2280

gcuggacgcg cggaagauaa gaaccuggac cggcucuuug aggauauucc ucagcccugg     2340

ccuggcuuca gagaggaacu gggggaggcc cugcgcucua cgaccguuag ccauaaagcu     2400

gaccacggaa gaaagcggcg ccccuccaga gaucgggacg ugacagccgc ccgccuucac     2460

aaugauaccg cuuauggccu cacaggggac gucgccgcgg auggcaaaac cccgaucgug     2520

gugcauagag uuccucuggc uucucugaag cccgccgacc ucaucgaucc ccugcggauu     2580

ccugacccgg uccuuagaga cgcccuguac gcugccacgc gcgaucggac aggaaaagaa     2640

uuugagcagg cgcucaccca auucgcuaga caccaucccc uguuuaaggg cauccgccgg     2700

gugagagugc gggaaccucu gaacgucauu cccauccgcg acgccgccgg gagagcuuau     2760

aaagccuaca agggagaugc uaaugcccgg uucgacgugu ggcgccuucc uaacggcaag     2820

uggguuagcg auauuguguc cauguucgac gcgcacugua uggccgauaa aagcgacaga     2880

cggccccauc cugcugccaa gaaaguccug ucucuccgcc agaaugauau gguggcuauc     2940

gagagauccg ggguuaccca gaucgcccgg guggucaagu uuagcacaaa cggaucuauu     3000

gcccuggcug cggacaacga ggccggcgcu cucaaagccc gcgaugccgc ucccaaugac     3060

guggauccgu ucaaguauau cuuuuccagc gggggcggac ugaagaaccu uaaagccaga     3120

cagguucggg uggacccucu gggcagaguc uucgaccccg ggccucgcga auga           3174


<210>  3815
<211>  3177
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3815
augaagaaca gcuacauccu gggccucgac guggggauug ccuccgucgg auauggccug       60

auccacucug auagcaaauc cguuauugac gcugggguga gacuuuuccc cgaggccaau      120

guggaaaaca augagggacg gcgcgcgaag agaggcucuc ggcgccugaa aagacggcgc      180

auccauagac ucgaucgggu caaguaccug cuugcucagu aucaccugau uaucgacgau      240

aaaaucccua agagcaccaa cccguacgcc auucgcguga aggggcucuc cucugcccug      300

agcaaauccg aacugguuau cgcucuccug caucuugcca agagacgggg cauucacaau      360

gugaacgucg ugauggacga gaaugaaagc acaaacgagc ugucuacgaa agagcaacuc      420

aaggaaaaug cuaaacagcu ggagaaccgc uauguuugcg aacuucagcu ggauagacuc      480

aaugagcagu acaaggugcg gggagagcgc aacagauuua agaccgaaga cuucguccgg      540

gaggccagac aaauccugac aacccagcag aaauaucaug auauugacga acacuuuauc      600

gagcaguaca cacaccuucu cgaaacccgc cgggaguacu augagggccc cgggaaggga      660

ucccaauacg gcugggaagg ggaucugaaa cagugguaug agaugcuuau gggaagaugu      720

acauacuucc cugaagaacu gcgcagcgug aaguaugcgu acaccgccga ccucuuuaau      780

gcucugaacg aucuuaauaa ccuggugauu gcccgggacg aaaacgagaa acucgaguau      840

uucgaaaagu accauaucau cgagaauguc uuuaaacaga agaagucucc cacgcugaaa      900

cagauugcua aggaaaucga cguuaaagag uccgauauua agggcuacag aaucaccaaa      960

aacgggaagc cucaauucac cagcuuuaag cuguaucacg accucaauaa aauuuuccug     1020

gauaagaaaa accuugaaaa uaucgagcug cucgacgaga ucgccuuuau ucugacacuu     1080

uaccaggauc cccugucuau caaggaagcg cucgaccagc ugcaagaucu gcucacagag     1140

uccgaaaaag aggccauugc ucaucugaac ggauauaccg gcacgcaccg ccuuagccug     1200

aagugccucc aucugcugau cgacgagaug uggcagacau cccggaauca gauggaagug     1260

uucaccgccc uuaaccucaa gccucagaaa uaccaacugu cuaagcagaa acagauuccg     1320

aagaaaaugg uggaggaauu uauccugagc cccgucguga agagauccuu cauccagagc     1380

auugagaucg uuaauacaau uaucaagaaa uauggccucc cugaagagau uaucauugag     1440

cuggcucgcg aaaagaacuc ugaggaucgg agaaaauucc uuaaucgcau ccaaaaggaa     1500

aacgagaaua cccggaaaca gguggaagag gucaucagag aguacgggaa ccggaaugcc     1560

aagggacugg uggagaaaau uaagcuccac cagaugcagg agggcaagug ucuguauucc     1620

cugcaaaaca ucccccuuga cgaucuccug cgcaacccua gccacuacga aauugaccau     1680

aucauuccca gaucuguuag cuuugacaau uccuucaaca auaaagugcu ggucaagcag     1740

gaggaaaacu cuaaaaaggg gaaucggacc ccguaccagu aucucaacag caaugagucc     1800

gugcugagcu acguccaguu uaaacaacac auccuuaacc ugucuaaguc caaggagaga     1860

aucagcaaaa agaaacgcga auaucuccug gaggaacggg auauuaauaa guucgaggug     1920

cagaaagagu uuaucaacag aaaucugguu gacacacgcu acgccacgag agaacuugug     1980

cagcuccuga agucuuauuu cuccgcuaac gaucuggacg ucaaggugaa aaccauuaau     2040

ggcagcuuua caaacuaccu ccggaaggcg uggaaauucg auaagucccg caauaaagga     2100

uuuaagcauc acgccgagga cgcucugauc auugccaacg uuucuuaucu uuucaagcaa     2160

agcaaagaac ugaaggaggc caacgcuauc cucgaaaaua acaaucuguc ccugaacaaa     2220

aauaaggauu cuaaaaaccu ucucaccgag caggacuaca gaaagauguu ugagaucccu     2280

aaacaggugc aggauauuaa ggacuacaag gauuucaaau auagccaccg ggucgacaag     2340

aaacccaauc gccaacugau caacgauucc cuguacagca caagagaagu gaaugagaac     2400

cauuauauug ugcagaccgu uaaggacauc uacgccaaag acaauacgaa gauuaaggau     2460

cucuuucuga aaacaccuga aaagcugcug auguaucagc acgaccccca gaccuucgag     2520

aaacucaugc uggucaucaa gcaauacggg gaagagaaaa accccuucgc gaaguauuac     2580

gaggaaaaug gagaguaccu gaccaaguau gcuaaaaacg aaaagggccc uacaaucaaa     2640

cggcuuaagu acauuggcaa aaaggugggg acccaucugg auaucucuac aaauuuuucc     2700

ggagccaagc gcaaaguggu ccagcucagc auuaagccgu ucagauuuga cguguuccac     2760

ucuaaagccg gcuaucacau gcugacgcuc accuacaugg auaucaagaa aaaggagaag     2820

cauuaucaga uucugaaaaa cgaauaccag gcucuuaagg agcgguauaa aguucccucc     2880

gacgcccaau uuaucgggag cuucuacuac aacgaucuga uuucuaucaa ugacgagcuc     2940

uuucgcguga ucggagucaa caauagcaca agaaacgugg uugaacugaa uaugguggau     3000

auuaacuaua aggacuacug cgaucugaug ggcaucaaaa agaccccucg gauuuucaaa     3060

acaaucacca agaagacggg gaaaauugag aaguauucca ccgacauccu uggcaaucag     3120

uuuaaaguca agcccagcaa aucuccucag cugaucuuca agagaggagu gcucuga        3177


<210>  3816
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3816
augagacggc ugggccucga caucgggacc aacagcauug gauggugccu gacacagggc       60

cccggggaug aggccgacgg aaucguggau auuggcgucc gcaucuucuc cgacgggaga      120

gauccucaau cuggaacgag ccuugcuguu gaccggcgcg augccagagc ggcucggcgc      180

agacgggacc gcuacauugg cagacggucc gccuuucugg auacccucgu ggcccacggc      240

cugaugccgg cugacgccga ugaagcuaaa cuuguggccg cgcuggaccc cuauacacuc      300

cgcgccagag cucuggaggc cccucugaca ccccaugaaa ucgggcgggc ucucuuccac      360

cugaaucagc gcagaggauu uaagucuaac cggaaggccg agagaaaaac caaugaugcg      420

gacgacggca agaucgccac cgcugccuac gcucuugauc gcgccaugcu ggacggggga      480

uucgauacac ucggccaguu ucugaacgcc caggaaacca aacgggucag aauggacggg      540

gaggcucaag gauaugauuu cuacccucag cgccggcaug uggaguauga auuugacaga      600

auuuggacgg agcaggcgaa gcaccauccc gcccuucuga ccgaaacagc ucgcuacgcc      660

cuccaccgga uccuguucua ucagagaccu cugaaggccc aaguuguggg caaauguacc      720

uuuguccaca gcgaggaucg ccucgcuaag gcccauccgc uguuccagca gcggagacuu      780

uacgaggaag ugaaucagcu ggagguuaca acccccggcg aagcgaaccg caaacucacg      840

cuggacgagc gggauagacu ugugcgggaa cugucccaca agaaagcuau ugccuuuaca      900

ucucucgccg cuaagaucaa gcuggagccu gggcagagcu ucaauaaagc cuccgagacc      960

cgcacacaac uggcuggaga cgaagucaga gccgugcucg gcgauaagaa acgguuuggg     1020

cccgcguggg agcauuucga cagagaccgc caguggacca uuguggaacg gcugcuugag     1080

gaagaggauc cugacgcccu gcacgcuuuc cucacccaag auugcggacu ggacgaugag     1140

cacgccgcug ccacagcgaa gacgagccuu cccgaaggcu acgggagacu gggagccacc     1200

gcuacagccc gcauccucgc ugagcugcag gccgaaguca uuaccuauga cgaggccguu     1260

agacggugug gcuggcauca cucugaucau cgcacaggcg aaauccugcc gcagcucccu     1320

uacuaugggg agcugcuuac cagagacauu cccccuggaa cgcaggaucc caacgauccc     1380

ccugagaaac gguggggccg caucacaaau ccgaccgugc acaucgggcu gaaccaagug     1440

agacagcucg ucaaugcuau uguggcgguu cauggacggc ccgaccagau cguggucgaa     1500

cuggcccgcg agcugaaacu uaacgaaaag gacaaggagg agcacaauag acggauucgc     1560

agagauaccg cugccgccau caagcggucc gaaaaacuca cacaggaggg cuuuccugac     1620

accggggcua acagaaugcu gcugcgccuc ugggaagacc ugaaucccgc caacccucuu     1680

gaucggagau gccccuacug uggcgcgccg aucggaaugc gcgcucuguu cgccggcgag     1740

gccgacgugg aucacauuau cccuuauagc cggucucucg acgauuccgc ugggaacaag     1800

guuguggccc auagaagcug caaucgcgcg aaaggaaacc ggacacccua cgaaaagugg     1860

ucugacgauc cugacagaug ggaugcuauu acggcccaag ucgcccgccu gcaccggucc     1920

aaacagugga gauuuggccc cgacgcuaug gcccgggugg agaaggaugg gggauuccug     1980

agccgccagc uuaccgacac acaguaucuc uccagacugg cuggccggua ccugucuagc     2040

cucuacccua ccaaggagga aggguccguc uaugugaucc ccggccgcau gacagccaug     2100

cugagacggc uuuggggacu gaaugauauu cucagcgacc auaacaucgu ugagaauaaa     2160

cacucuaacg cgccgaagaa uagacuggac caccgccauc acgccaucga ugcugccgug     2220

gucgcugugu ccacccggag ccugaugcaa agaauugccc gcacggccgg ccaggcugaa     2280

gaccggaacc uugauagacu cuuugagggg cuggacccuc ccuggcccgg auuccgcgau     2340

cagcugcggg acagacuccu ggcgguuacc gugucucaua aagccgauca cggccgcaca     2400

gggaccccug ucccgggaaa ggacgcuaca gccggcagac uucauaauga uaccgccuac     2460

gggcugacgg gcgaaacaag cgcuaaagga cuccccaucg uggugcaccg gguuccucug     2520

accucccuga agcccgccga cauuacagau ccugaccgca uccccgaccc uguccuuaga     2580

gaugcgauug agcgggcuac cgccggcaag auggggaaag acuuuaccga ugcccucgcu     2640

aaguucggac gcucuggcgc ccaggagaca gacagaaaag gggaacggcu guuuagagga     2700

acgcgccggg ugagagugcg cgagccccug ggcgucaucc cgauugcgga ugcuagcggg     2760

cggguguaua aggccuacaa aggcgacucc aacgccagau ucgauguuug gcggcucccu     2820

gacggaaagu gggugagcga uaucgucucu auguuugacg cucaccgccc cgaucaaggc     2880

gacagacggc cucaucccgc cgcuaagaaa gugcuguccc uucgccagaa ugauaugauc     2940

gccauugaaa gagacggggc gccgcgggag aucguucgcg uggucaaguu cagcaccauu     3000

ggaucugugc agcuggccgc uacaaccgaa gccggcccuc ucaaagcuag agacgccucc     3060

ccucccgaug uugaccccuu ucgguauauc aacagcuccg ccgggggacu gaagaaaauu     3120

aaggcucgcc aagugagaau cgauccccug ggccggaucu ucgacccugg cccgagauga     3180


<210>  3817
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3817
augaucgagc ggauucuggg cguggaccuc gggaucagcu cccugggaug ggccauuguc       60

gaauacgaua aggacgauga ggcugccaac agaaucauug acugcggcgu ucgccuuuuc      120

accgcggcug aaacacccaa aaagaaagag ucuccuaaua aggccagacg ggaagcccgc      180

gggaucagac gggugcugaa ccgcagacgg gugcgcauga auaugaucaa aaagcucuuu      240

cugagagcug gacuuauuca ggaugucgac cuggauggcg aagggggaau guucuauagc      300

aaggccaacc gggcugacgu gugggagcuc cgccacgaug gccuguacag acugcucaaa      360

ggcgacgaac uggcccgggu ucuuauccau auugcgaagc accgcgggua uaaauuuauc      420

ggagaugacg aggccgauga agaguccggc aaggugaaaa aggcuggggu cgugcugaga      480

caaaauuucg aggccgcugg augucggacg guuggcgaau ggcucuggag agagcgcggg      540

gccaacggaa agaaacggaa uaagcauggc gacuacgaaa uuucuaucca cagagaccug      600

cuuguggagg aagucgaggc gauuuuugug gcccagcagg agaugcgcag caccaucgcu      660

acagaugccc ugaaagcugc cuaucgggaa aucgccuucu uugugagacc gaugcagcgc      720

auugagaaga uggucggcca uugcaccuac uuccccgagg agcggagagc uccuaaaucc      780

gcgcccacag ccgaaaaguu uaucgcuauu ucuaaauucu uuagcaccgu uaucauugac      840

aacgaggggu gggagcaaaa gaucaucgaa cgcaagacac ucgaggaacu gcuggauuuc      900

gccguguccc gggagaaagu ggaauuuaga caccuccgca aguuccugga ccuuagcgau      960

aaugagauuu ucaaaggacu gcacuauaag ggcaaaccua agaccgccaa gaaacgggag     1020

gcuacgcucu uugaccccaa cgaaccuacc gagcuggaau uugauaaggu cgaggccgag     1080

aaaaaggcgu ggaucucucu uagaggggcu gccaaacugc gggaagcccu cggaaaugag     1140

uuuuacggcc gcuucguggc ucuggggaag caugccgacg aagcuacaaa gauucugacc     1200

uacuauaaag augagggaca gaagagacgg gaacucacaa aacugccgcu ugaggccgag     1260

augguugaaa gacuggugaa gaucggcuuu uccgacuucc ucaaacugag ccuuaaggcg     1320

auucgcgaua uccugcccgc cauggagucu ggcgcucggu acgacgaagc cguccucaug     1380

cugggggugc cucacaagga gaaauccgcu auccugcccc cucucaacaa gaccgacauu     1440

gauauccuga aucccacggu uauuagagcc uuugcgcagu uccgcaaagu ggccaacgcu     1500

cuugucagaa aguauggagc cuuugaccgg gugcauuucg aacuggcucg cgagaucaau     1560

acaaaaggcg agauugaaga uaucaaggag agccagagaa agaacgaaaa ggagcggaag     1620

gaagccgccg acuggauugc ugagaccucu uuucaagucc cgcucacacg caaaaacauc     1680

cugaagaaaa gacuguacau ccagcaggau gggcggugug cguauaccgg agacgugauu     1740

gagcuugaac gccucuucga ugagggcuac ugcgaaaucg accacauucu gccuagaucc     1800

cggagcgccg augacuccuu ugcuaauaag guucuguguc ucgcccgcgc caaccagcaa     1860

aagaccgaua gaacacccua ugagugguuc gggcacgacg cugcccggug gaaugcguuc     1920

gagacgagaa ccucugcucc uagcaaccgc gugcggacag gcaagggaaa gaucgauaga     1980

cugcuuaaaa agaauuuuga cgaaaacucc gagauggccu ucaaagaccg caaucugaac     2040

gauacccggu acauggccag agcuauuaag acauacugcg aacaguauug ggucuuuaaa     2100

aauagccaua ccaaggcccc cgugcagguu cgcucuggca agcucacguc cgugcugcgg     2160

uaccaauggg ggcuggagag caaagacaga gaaucucaca cacaucacgc ggucgaugcu     2220

aucaucauug ccuucagcac ccagggaaug gugcagaaac uuuccgagua uuaccgcuuu     2280

aaagagaccc aucgggaaaa ggagagaccc aaacucgccg ugccucuggc uaacuuccgg     2340

gacgccguug aagaggcuac acgcaucgaa aauaccgaga cagucaagga gggcguggaa     2400

gugaagagac ugcucauuuc ucggccgccc cgcgccagag ucacggggca ggcgcacgag     2460

caaaccgcca aaccuuaucc ccggaucaaa caggugaaaa acaagaaaaa guggagacug     2520

gcuccuauug augaagagaa auuugagagc uucaaggccg accgcguugc uuccgccaau     2580

cagaagaacu uuuacgaaac aagcaccauc ccccgggugg augucuauca caaaaaggga     2640

aaauuccauc uugugccgau cuaccugcac gagaugguuc ucaaugaacu gccuaaccug     2700

ucucuuggca caaaccccga ggccauggac gaaaauuucu ucaaguuuuc cauuuucaaa     2760

gaugaccuca ucagcauuca gacccaaggg acgccuaaga agcccgcuaa aaucauuaug     2820

ggcuacuuca agaacaugca uggagcgaau auggugcugu cuuccaucaa caauagcccu     2880

ugugagggcu uuaccugcac acccgucucc auggauaaaa agcacaaaga caaguguaag     2940

cugugcccgg aggaaaacag aaucgccggg cgcugucucc agggauuccu ggauuauugg     3000

ucucaggagg gccuucggcc ucccagaaaa gaauuugagu gcgaccaggg ggugaaguuc     3060

gcucuggacg uuaaaaagua ccaaauugau ccccucggau auuacuauga agugaaacag     3120

gagaagcgcc ugggcaccau cccucagaug cggagcgcca agaaacuggu caagaaauga     3180


<210>  3818
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3818
augagcaaga gacugcggua ccgccucgcc cuggaccuug gcaccaacuc caucggguuc       60

ugccugcucg aucugaaugg agacggcaga cccugugggg ugcggcgcau gggagucaga      120

auuuuuccug auuucucuaa acuucagcaa agccuggcug ccgaccggcg ccucgcgaga      180

uccaugcggc gcagacggga ucgcuaucug agacggcgca aggcucugau gaacgcccuc      240

guugccgcug cccugaugcc ggcugacccc aaagccagaa aggcgcuuga ggcccuggau      300

ccuuacgaac uccgggcugc cgcuguggcc ggcccccugc cugcgcacca gcuugggaga      360

gcccuguuuc aucucaauca gcgccgggga uucaaaucua acagaaaggc ugccggcgac      420

gagaaugaag cuggcaaggu gaaaagcggg aucuccgccc ugaaggccga gcuggcugcg      480

ucuggagccg gcacacucgg gcaguggcug gcugcccgcc acgccaaccg gcaacccguc      540

agagcucgcc uuacgggaac cggcgccaaa gcgacauaua gcuuuuaccc ugaacgggcu      600

cuggugcaug aggaguucca ggccauuaga gccgcucagg ccccgcacca ucccgggguu      660

accgcugaac agugggaucg caucgagucc gugauuuuuc accaacggcc ucucaagccc      720

gucaaaccug gaaagugcac acuguauccc gaagaggaga gagccccgug ggcgcuuccu      780

aucgcccagc gcuuccggau ccugcacgaa cuccauaauc ugagaauugu gcggcccggc      840

cagggcgagg aagggcugag cccugcugac uuugaucgcu ucuuugacag acucugucgg      900

aagggagauc ugaccuucaa aacacuugcc aaggagcuga aacucccucc cgacgcuaga      960

aucaaccugg aagacgauaa gcgcgaccgg cuuaaaggcg augccaccgc ggacgcccug     1020

ucugcugaga aggggaaaag acgcagaauu ggagccgcuu ggcacuccuu ucccgccgau     1080

cggcagcgcg agaucguugg cagacucauu gacgccgaag augaggggga acuggcugcg     1140

uggcuggugg aggaacucgg acugccgugg gaggucgcca uccuuacggc ugagcggccc     1200

ggccugccug acggcuacug ccgccucagc gaaaaggccc uggagcaagu ggugccccug     1260

augcaugagg aacaccuucc uuaugaugaa gccgucagag cucucgggcu gcaccauucu     1320

gaccaccgga ccggagaguu ccuggaugag cuccccuacu acggccugcc gcuugaacgc     1380

uucauugccu uugggacagg agaggcggac gacccucccg aacugagaau cggccgguuc     1440

ccuaauccca ccguucaugu ggcucucaac gagcugcgca gaguggucaa ucggaucauu     1500

agacgcuggg ggaagccuga ggaaaucgug guugagcugg cccgggaucu uaaacagucc     1560

agagccgacc gcgauaagac acagcgggaa caagcugaca accagaaaaa gaauuggacc     1620

agaacgcagc agcuccgcga gcugggcaug gaacccacag gagaggcccu gcuccggcug     1680

agacuuuggg aggaacugcc gcgcauuggc agcgcgcacg ugugugucua uaccgggcaa     1740

ccuaucucua ugacacagcu ccugcaggga gauguggacg uugaucauau ucugcccuuu     1800

ucccggaccc uugcuaacgg cgccgacaau aaaaucgugu gccacagacg ggccaaccgc     1860

aucaagagaa accggacccc cugggaggcu uucggggucg ugagcgccga uggauacgac     1920

ugggcugcca uugcggaacg cgccgagucc cucccuaaga auaaaagaug gcgguuucug     1980

ccgaacgcua uggaaagauu cgagacagag cgcggcuuua ucgaucggca gcuggucgaa     2040

acgagacacc ucucucgcgu ggcccgggag uaucuggggu guauuguucc ccuugaaaag     2100

gugagagcug ccccuggccg ccugaccgcc augcuccggg gaagaugggg ccugaauagc     2160

auccuguccg acgcuaaccu uaaaaaucgc agcgaucaua gacaccaugc ggucgacgcc     2220

gcugugcucg ggguuauuga ccaacggcug gugcagcgcg ucgccgaugc cgcuaagaga     2280

gccgaggaaa aagagcugga gaaacucuuc gacggacugc ccgauccuuu ugaaggccuu     2340

cccgggccuu cuuuccggga ccaggugcag gagcgcgcgc aaggaaucau cgugucccac     2400

aagcccgaac acggcuggca ggggagaaug cauaacgaua cagcuuacgg cccgguugag     2460

cggcugccug acggaaccca cagagucgug acacgcaagc cccucggcag ccuggccgag     2520

aaggauuuug ggaauauucg ggacagagcc cuggcugccg cgcuugaacg ccauguggcu     2580

gagcaggccg cccagggacg gucucugggc gaagcucuca gcugcuucac cuggacaggg     2640

ggagagguca gacgggugcg caugcuggau aaacucggcu ccccuguugc cgugcaagac     2700

gaugacgggc acacguuuaa gcuguauaaa ggcgauucua acgcuuggua cgaaauccuu     2760

agagacggac ggggccgcug gaccgggaga auugucagca ccuaugaggc cuaccggcug     2820

caugcggagu ggcucgccga acgcggagac ggcgggagau ccgaugcugc cuuccccgac     2880

ugggcugccc ggcgccugug ugccggaauc ggcaagccgc uguucagacu uuuuaagggc     2940

gauaugcugg cuuucgggac agcgccuaau cgggagauua ugaaaguggu uaaguuuagc     3000

gaaggaagaa ucguguucgc cggccaccgc gaggcuggga accucaaaga acgggacgcc     3060

gauaaggacg aucccuuuaa auacagaacc ugcucuccug aggcccugcg caaggcucag     3120

gcccggcccg ucggagugga ccccgcgggc uucguucacg auagagggcc ucucgaguga     3180


<210>  3819
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3819
augggcauca gauucgccuu ugaccugggg accaacagca uuggaugggc uguguggcgg       60

acaggccccg gggucuucgg agaggauacg gccgcguccc ucgacggcuc ugggguucug      120

aucuuuaagg auggacgcaa uccuaaagac ggccagagcc uugcuaccau gagacgggug      180

ccgcgccaau ccagaaagcg gcgcgauaga uucgugcugc ggcgcagaga ccuccuggcc      240

gcccuucgga aagcuggccu guuucccguc gauguggaag aggggcgcag acucgccgcu      300

acagacccuu accaccuccg ggccaaggcg cuggaugaau cucucacacc ccaugagaug      360

ggacgcguua uuuuccaccu gaaccagaga cggggcuuua gaagcaaucg caaagccgac      420

cggcaggaua gagaaaaggg gaagaucgcu gagggaucca aacgccuugc cgagacacug      480

gcugccacca acugccggac acucggcgag uuccuguggu cuagacaucg cgggaccccu      540

cggacgagaa gccccacccg cauucggaug gagggagaag gcgcgaaggc ccuuuaugcu      600

uuuuacccua caagagagau ggugcgcgcc gaguucgaac ggcuguggac cgcucagucc      660

agauuugccc cggaccuccu gacacccgag cggcacgaag agaucgccgg cauccuguuc      720

cgccaaagag aucucgcucc ucccaaaauu ggguguugca ccuuugaacc uagcgagcgg      780

agacugcccc gcgcgcuucc gucugucgag gcccggggaa ucuaugaaag acuggcucau      840

cuccgcauua cgacaggccc uguguccgac agagggcuga cccggcccga gcgcgacguu      900

cuugccagcg cccugcucgc uggaaagucu cugacauuca aagccgugag aaagacccug      960

aagauccucc cucacgcgcu ggucaauuuc gaagaggcug gcgaaaaagg gcuugaugga     1020

gcccugaccg ccaagcuccu guccaaaccc gaccacuacg gcgcugccug gcauggccuu     1080

agcuuugcug agaaggauac auucgugggg aaacugcucg acgaggccga ugaagaacgg     1140

cugauucgca gacuggugac ggaaaaccgg cucucugagg acgcggcccg cagaugugcu     1200

uccauccccc uggccgaugg auauggccgg cuugggcgca ccgcuaauac agaaauucug     1260

gccgcgcucg ucgaggagac cgacgaaaca ggaaccguug ugacguacgc cgaggcugug     1320

agacgggccg gcgaaagaac agggcgcaac uggcaccaua gcgaugagcg ggacggcguc     1380

auccuggaua gacugccuua uuacggagag auccuucagc gccacguggu uccgggcucc     1440

ggggaacccg aggaaaagaa ugaggcugcc cgguggggaa gacucgccaa cccuaccgug     1500

cacauuggcc ugaaucagcu gcgcaaaguc gugaaccggc ucaucgcugc gcaugggaga     1560

cccgaccaga uuguugugga acuggcccgc gagcuuaagc ugaaucggga gcaaaaggaa     1620

agacucgacc gggagaaccg caaaaauaga gaagagaacg aacggcgcac cgcuauccug     1680

gccgagcacg gacagagaga uacagccgag aacaagauuc ggcugagacu uuuugaggaa     1740

caggcucgcg ccaaugcggg caucgcucuc ugcccuuaca ccgggcgggc caucggcauu     1800

gccgaacugu ucacaucuga ggucgaaauc gaccauauuc ugcccgugag ccucacgcug     1860

gaugacuccc uugcuaacag aguccugugu cgccgggagg ccaauagaga gaaacgccgg     1920

cagaccccgu uucaagcguu cggagcuacc ccugccugga acgauaucgu ggccagagcu     1980

gccaagcugc ccccuaauaa acgcuggaga uuugaccccg cugcccugga acgguucgag     2040

cgcgaaggcg gguuucuggg aagacagcuu aacgagacca aguaucucag ccggcuggcg     2100

aagauuuacc ugggcaaaau cugcgauccu gaccgcguuu augugacacc cgggacccuc     2160

acgggacugc uuagagcccg guggggccuu aauucuaucc ucuccgauag caacuucaag     2220

aauagaucug accaccgcca ucacgcuguc gaugccgugg uuauuggggu gcugacccgg     2280

ggcaugaucc agagaauugc ucacgacgcc gcccgcgcug aggaucaaga ccuggaccgg     2340

gucuuuagag augugccggu gccuuucgaa gacuuucggg aucauguucg cgagagaguc     2400

agcacaauca ccguggcggu gaaacccgaa cacggaaagg gcggggcccu ucaugaggac     2460

acauccuacg gacucguccc cgauaccgac ccuaacgcug cccugggcaa ucuggugguu     2520

cggaaaccga uucgcucucu cacggccggg gaaguggaua gaguccggga ccgcgcucug     2580

agagcccggc uuggagcgcu ggcugccccc uuccgcgaug agagcggcag agugcgggac     2640

gccaaggggc ucgcucaggc ccuggaggcg uucggcgcug aaaacggaau cagacgcguu     2700

cggauucuga agccugaugc cuccgugguc acaaucgccg acagacgcac cggcgugccc     2760

uaucgggcug uugccccugg ggagaaucac cacguggaua ucguccagau gagagacgga     2820

agcuggcgcg gcuuugcugc cucuguguuc gaagugaacc ggcccgggug gagaccugag     2880

ugggaaguca aaaagcuggg aggcaaacug guuaugcgcc uccauaaggg cgacauggug     2940

gagcuguccg auaaagacgg gcagcggaga gugaaggucg ugcaacagau ugagaucagc     3000

gcgaaucgcg uucggcucuc uccccacaac gauggaggca agcugcagga cagacaugcc     3060

gaugcugacg auccguuucg cugggaccuu gccacaauuc cucugcucaa agauagaggg     3120

uguguggcug uccgggugga ccccaucgga guugugaccc ugcgcagauc caacgucuga     3180


<210>  3820
<211>  3183
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3820
auggccguca agagccugaa cuacauccuc ggccuggacc uugggauugc uuccugcgga       60

ugggccgugg uugagaugga ugaacaggag aauccccuga gacucaucga cgugggcgug      120

cggaccuucg aagaggcgga aaacccuaaa gaugggucua gccuggcuga gacacgcaga      180

cuugcccggg cccaacgcag acugauuucc cggcgcgcua auagaaugaa caagcuccgg      240

cgccugcuga aacaggagaa uguccuccag gccaacgacu augaugaaaa gggacugauc      300

auuggccuuc cgaaucaggc uugggagcug agagcccaag ggcucgaccg gaaacuggaa      360

cccaaggagu gggcggccgu gcuuauccac cugguuaagc aucgcggaua ccucucucag      420

cggaaaaacg agagccagac ggcugauaag gaacugggcg cccugcucuc cggcaugaau      480

cacaaccauc agcugcuuca aucucagagc aaucaguaua gaaccccugc ugagcuggcc      540

aucaaacagu uugcgacaca agacgggcac auucgcaacc agagaggagc cuacacccau      600

acauucaauc ggcucgaucu gcagaacgaa cuuaaucaac uguuugacgc ucagaccaga      660

cucggcaacc cccacguguc cgggagccug aagacagcca ucaacgaucu gcucaugaau      720

cagaaagcug cccugucugg agacgccauu cuugagaugc ugggccgcug uaccuucgaa      780

ccuuccgagu auaaggcugc gaagaacacg uacagcgccg agcgguuugu cuggcucggg      840

aaacugacca aucuuagaau ccaggauaac ggacuggaau cugcucucuc ccgcgagcaa      900

cgggaacugc ugcucaauga gcccuauaac aagacaaaac ugaccuacca acaggugaga      960

aaacuucugc gccucccuga aacagccuuc uuuaagggcc ugcgguacgg caaggacaau     1020

gccgagagcg cuacccugau ggagaugaaa ucuuaucacg ccauuagaaa gucccuugaa     1080

aacaagagcc ucaaauccga gugggaaaag cugaaaacgc agccgaagcu gcucgaccug     1140

aucgggacag cguucucuau uuacaaaacc gaugacgaga ucgcuggaug ccuuaagaau     1200

cagcugcccg aaaacguuau cauugcccuc caggagcagc ugaauuuuag caacuucaug     1260

gagcuguccc uuaaggcccu cgaaaaacug cugccucuca uggagcaggg ccugcgcuau     1320

gaugaggcuu guacacaaau cuacgggaau cggcaugaaa agauugccga caaaaccgag     1380

aaguuucuuc ccaacauccc ugcugauaaa cugagaaauc ccgugguccu ccgcacccug     1440

acacaggccc ggaaggugau uaacgcgauc aucagacggu auggaagccc ggcccgcgug     1500

cacauugaaa cgggcagaga ggucgggaag ucuuucuccg accgggauaa acugaagaaa     1560

cagcaggaag agaaccaaga acagagacgc cgggcuaucg acgaguggaa gcaguuuuuc     1620

ccuaaugccg cucaugagcc caaagccguu gauauucuua agcucagacu guaccaacug     1680

cagcagggaa agugccucua uagcggccug ccuaucgacg ugcgcagacu ucccgaaaaa     1740

ggcuacgugg agauugauca cgcgcugccc uucucucgga ccugggacga cagcuuuaac     1800

aauaagaucc ucguccuggc cuccgaaaac cagaauaaag ggaaccaaac accuuacgag     1860

uaucugcgcg gagcugauaa uucugaaagc uggagacuuu ucguggagaa cguucagaau     1920

ugucaguuuu ccccgaacaa gaaacagaag aucauggcca aacaacucga cgagaagggc     1980

uuccuggaac ggaaucugaa cgauacccgc uacgugagca gauuucucug ccaguucauu     2040

ucugaguauc ugcaccuuac agggaagggg aaaaagcggg ucuuugcuuc caauggccag     2100

aucaccgccc ugcuccgcgg gagauggggc cugagcaaaa accgggaaga gaaugaccgc     2160

caucacgccg uggaugcugu uguggucgcg uguucuacgg uggccaugca gcaaaagauu     2220

acacaguucg ucagauacca gaaaggaaac guguuuaccg gcgaacggau cgacaaggag     2280

accggggagg uuauuuccau ccauuucccc acaccuuggg aacacuuuag agaggaagug     2340

augauucgcg ucuucgauga caacccccug cggacccuuc cugagaagcu gcccaauaga     2400

ccggaggcuc uccaugaaua ugugaugccu cuguuuguua gccgcaugcc cacacggaaa     2460

augacgggac aaggccacaa ggagaccauc aaauccgcca agagacucga ugagaaaauc     2520

ucugugcugc gcgucccucu uacacagcug aagcucaacg accuggaaaa uauggugaac     2580

cgggaaagag agcccgcccu guacgaggcu cuuaaggccc gccuggaaca guucaaaaau     2640

gauccugcga aggcuuucgc cgagcccuuu uucaaacagg gaggacaaca ggugaaggcc     2700

guucgggucg aaauugugca gaaaagcggc augcuccuga gaaagaucaa cgggguggcu     2760

gacaauggcu ccaugguccg gguggauguu uuugagaagu cuggaaaauu cuaucucgug     2820

ccgauuuacg ccuggcaggu cgcggaaggc auccugccua accacgcugu gguucauagc     2880

aaggacgagu ccgacuggau ucuuauggau gagagcuacc acuuuaaauu cgcccuguau     2940

aagaaugacc uccuggaagu gcaaaccaaa aaggggugca ucaugggaua cuuugccucu     3000

cuggaucgcg cuacaggcgc caucaccauu agagagcaug accgggauaa aaagaagugu     3060

aaagacgggg aacaccgcuc caucggaguc aagauggcua gcucuuucaa gaaacuucag     3120

guggaugagc ugggcaaaaa cauucacgaa acgagauccc ggaagagacc caauaucggg     3180

uga                                                                   3183


<210>  3821
<211>  3186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3821
augcacaccc ugcccuacac acucggccug gacaucggga uugccagcgu gggagcugcc       60

cuucuguccg augagucuau ccucggcaug caugucagaa cguucagcgc ggcugaaacc      120

gacaaggagg gggauccucu gaacaaaauu cggcgcgaag ccagauccgc ccggcgcaga      180

auccggcgca gagcucaccg gcuucugcgc cucagacggc ugcugaagcg cacaggacuc      240

auuucugaca ccagccugga gguucuuaaa acauccaaga ccccguggca gcugagaucu      300

gaaggccucg aucggcugcu uagccccucc gagcuggccg cugcccucua ucauaucgug      360

aaacaccgcg gguuucaaag caauagaaag gcggagcuga agacagacgc caaagcugga      420

cagaugcugu cuggcguguc cgccaaccag cagcucaugc gggauaagag cuacagaacc      480

aucgcugaaa uggucugcaa agacgaugcc uucaaggacc auaaacgcaa uaagggcggg      540

ucuuaucgga acacguuugc gagauccgau cuggccgacg agcuuaagac ccugcucaaa      600

aagcaacgcg aauacgauaa uccucuggcu acacccgagc uugaggaagc cgugcacacc      660

cugcucaugg cucggagacc uguuuucaaa gacgacgaua uugagaagcu ggugggauuu      720

uguacauucg aacacggcga gaaacgcgcc cccaaggccg cuuuuagcuc ugaacgguuc      780

aucuggcugg ggaaacucaa caaucugaga cuuauuuauc cuggagagca gcgcucccug      840

accccggagg aacggagcca gaucauuaac cuccccuuua cgcaggcguc cgucaaguuc      900

ucucaaauca gaaagaaacu ggaccuuccu gaguacgugc gcuuuaaucu gguucgguau      960

aagcccggcg aagagacaag caaauccgaa accgccacau ucuuugaggc uaaggccuuc     1020

caugagcuca gaaaaaccua ccgggaaagc gggauuucug agcuguggga acgcgaucag     1080

cacgacccuc agagacugga ucagaucgcc accgcuaucg ccguguaccg ggacgaugag     1140

agaauuagaa acgaacucag cccccauaca aucgagaaug aggucgugga agcggugcug     1200

aacugcgacu ucacgcaauu uauuaaucuu agccugaagg cucucaacaa gauccugccg     1260

uauauggagc agggacagcg guacgaugaa gccuguaccu cugccggcua ucaacaccac     1320

agcccucagc agaaugagga gaaauccggc cgcauucccc cucccgacaa gaacgucauc     1380

cgcaaccccg uuguguacag aucucugaau caggcucgga aacuuaucaa cgccauuaca     1440

aagcgcuaug gggcuccugc cgcgauccau cucgaaaugg ccagagaucu gagcaaaccg     1500

uucuccgagc ggaaguccau ugaacgcgag caagaaaagu acagaaaaca gaaggugcag     1560

cugcggaaug aguauaucga gcacuuugga accgaaccca acggccucaa ucugcuuaaa     1620

uuccgccugu acaaggagca ggacgcucaa ugcgccuaca cacagaaagc ucucgaccug     1680

ucuagacuga acgaagccgg guaugucgag guggaucaua uucuuccuua cucccggagc     1740

uaugacgauu cucuccugaa uaagacccug guucucacgg aagcgaaccg cgacaaggga     1800

aauagaacac ccuuugaguu caugggcggg gaugaccagu cccagcacug gcaaaaauuu     1860

gugcgguggg uccagaugaa cccuaagauc agacaggcca aacgcaaucg guaccugaga     1920

aaggauuuca gcgagaaagc ugcccgcgaa uuucgggaca gacaucuuaa cgauacccgc     1980

uauaucggac gggagcugaa ggcuaugauu gaaucccacg ugcaguggca ccccgacucu     2040

aaaagcaagg auuguugcgu ugugcucucc ggccaacuga ccgcccugcu uagaggccgc     2100

uggggguuca auaaggaccg ggccgcugga gaucuccauc acgcggucga cgccgcugug     2160

gucgccgccg cuacaagagg caugauccag cggauggccc gccauucuaa auuucacgag     2220

cugaagaacg ugagaaccag cuacgcggac ccggauacag gggaaguugu gaaucuggcu     2280

gccgcccggg accucgagaa acacuucccu cugcccuggg auaacuuucg caacgacacg     2340

cuugcuuauc ugaauccuuc cgccauuagc gcggaugagu cugcucagga aggacauaga     2400

ggcguccggg uguccagagc ccccacccgc cggaagcucg gggccgcuca ccaagagaca     2460

aucagaagca aaggcaagga caacauucug cuggaacagg gacuuucugc cguuaagacc     2520

ccucucucca aacugacacu gaagaauauc gagaacaugg ugggcuacga ggauccccgc     2580

aaugcugccc ucgucaaagu gcugcgggaa agacuugagg cguucggggg agacggcgcc     2640

aaggcuuucg ccccgaacca gccucccgug uacaaaccca gcaagccugg gaagcaggcu     2700

ccggaaauuc gcucuguuaa aauggccagc aaguccaaag gagcccugca uguccgggag     2760

ggccucgcug auagcgggag aaugauccgc aucgacauuu augaaaccaa gaauggcuac     2820

gugggagugc ccaucuaugu cucugauaaa gcgagaucca agcugccuaa ggccuacccc     2880

aaaaagggcg aggaccgggc uaacuggccu gagguggaua cgaaugacaa cuuucuguuc     2940

agccuuuauu ccaccgacug gauugaagcc aaaacaaaga aagagaccau cgccggguac     3000

guuaagucuu augauauuuu ugacggacgc cugcucgugu acgcucacga uaauaaaaag     3060

gucgugagaa agcugacacu cagcgcccug gcggaccuuc aaaaacugca uguuuccauc     3120

cucggcgauc uguacaaagg gggaaccccc aaguguucuc cucugcggaa gggccaggaa     3180

cuuuga                                                                3186


<210>  3822
<211>  3186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3822
augcugagac ucggccugga caucgggagc accuccauug gaugguuccu uuaccggauc       60

acaaacggca ugauugccga gaucauugau ugcggggugc gccuguuuuc ugacggaaga      120

gaugcuaaga cgggcgucag cuccgccguu gaacggcgca augcgagagc ucuccggcgc      180

agacgggacc gcuaucugag acggcgcgcc gcccuuauga gacggcuggc ugccgcuggg      240

cucaugcccg aggauccuuc ucaggccaaa ccgcuggaac ugcucgaccc cuaccgccug      300

agagcggaug cccuugaccg gagacuggag cucaccgaac ugggacgcgc ucuuuuccac      360

cugaaccaac ggagaggcuu uaagagcaau cgccagacag aucggggcaa caaugaaggg      420

ggaaaaauca ucgacggcuc cgccagacuc gaucaggcua ugauggccgu gggggcgcgc      480

accuauggag aguuccugca ucugcggaga ucucaggccg aagaccuccg ccgggugccu      540

agcgucagaa cacgccugac ccuucggucc guggauggcg ggaaggcuga gacaggauac      600

gacuuuuauc ccgacagacg gcaccuggaa gaggaguuca acaaaguuug ggccgcucaa      660

gccgcccauc acccugugcu caccgauagc cugcgccaug aagucuuuga gacgauuuuc      720

uuucagagac cccuuaaggc uccugcggug ggccgguguc uguucuuucc ggaacccaga      780

cucccucgcg cccacccccu gacccagcgg agaguucugu acgagacagu gaaugcucuc      840

cgcaucagaa ccgaaucugg cgccgccguc ccucugggga aggagcagcg ggacaaagug      900

auuaugcuuc uggauggaaa ggcucccgug aaagccaucg cguccgcuaa gcucagccug      960

aaagccauug ccaagcaccu uaagcugcgc gacggccaaa cauucacccu cgagaaaggg     1020

agagauuaua ucgcuugcga cccgguccgg gccucucugu cccauccuga ucgcuuugga     1080

cccagaugga gcguucugga agcugaggug caggccgaaa uugugggccg gauccgcgac     1140

gaugacgaua cgauggcgcu cgucgagugg cugaaacagg ugcacgggcu ugacgacgcc     1200

caugcuagag ccacagcuga ugccccucug cccgaaggau acggccggcu cggcgagacc     1260

gcgacacgca agcugcugga cugucuuaag gccgagguuc ucaccuacuc ugaagcugug     1320

gggaaagccc uggaucagca ccacuccgac agacggaccg gagagauccu ggaaagccuc     1380

cccuauuacg gcgagguccu gcaagcucau gugauuccug ggacaggaga accgcaccau     1440

gaugacauca cgagauucgg ccgcauuacc aaccccacag uucacaucgg gcuuaaucag     1500

cugcggagac ucgugaaccg cauuaucgcc cgguauggca agccugccga gaucgucgug     1560

gagcuggcua gagaucugaa acaguccacc ggacaaaaga aggcggccau ugcucuuaau     1620

cucaaaaacc gcaaggccgc cgaagcucgg ucugcccugc ugacagagug gggcgucccc     1680

gacaccgggg cgaauagaau guacgugcgc cucuggcagg aacugcauga ggauccuaug     1740

augcggagau gccccuauac gggaacaauc auuagcccgu cccuucuguu cagcggcgcu     1800

aucgacauug aucacauccu cccuuacucu cggacccugg acgauuccac cgccaaccgc     1860

acacugugua ugaaagaggc caauagacag aagcggaaca aaagccccuc ugaagcucgc     1920

agagacggga ugcggaucug ggcccuugag gacgauaacc ucagacacau uccugaauuu     1980

aagcgcuggc gguucgcgga ggacgcuaug gccagauuug aaggagagcg cgauuucgag     2040

gcccgggcuc ugguugacac ccaguaucug agcagaaucu ccaaggccua ccucgaaaca     2100

cuguacgaug gcgcugacgg gaaaucucau gugugggucg ugcccggccg ccuuacggag     2160

augcugcgga gacacugggg acucaauggc auucugaccg auccucugcg cgacguuaca     2220

aaggagaaaa acagaaugga ucaucggcac cacgccaucg acgcggccgu ggucgcugcc     2280

accgaucgcg ggcuuauuaa ggcuauggcc gagcaagcca gaacagacgu ggaucaggga     2340

cgggaagcuc ucgcgagcuc caucaugccc ccguggccug gcuuucgcga ggccguggcu     2400

gcccagguug acagaauuau cgucagccau cggcccgacc acgggagaau cgaucugacc     2460

acgcgcauga aaggacggga ccagaccaca ggcccccugc auagagauac cgccuauggg     2520

cucacaggcc aaucugagaa gggagugccu cuggugguca cccgcgaacc guucgacaau     2580

cuuacgccca caaugauugg caagauccgg gaugagccuc ugaaagcugc ccucgcggcu     2640

gugaccgccg ggaagaaaga cgaugccuuu gcugaagccc uggcgcacuu cagagaccgg     2700

cccggaccuu accauggcau ucgcagaguu cggcugaucg agcccauuuc cguggucgaa     2760

auccgcgaug cuagcgggag agccuauaag ggauacaaag gcaacucuaa ucacugcuuu     2820

gagguguggc ggcuuccugg gggcgccauc acagagcaca uuguuuccac cuucgacgcu     2880

caucagcccg gaccggaacc ucgcccccac ccugccgcua agagacugcu ccggcuguuu     2940

aagagcgaua uggugaaacu cgacgacucc aaguucggcc ccgucaucgc caccgugguu     3000

auguuugauc gcaacgggaa aauuucucug gugccgcaua cagcgagcaa ugccagaaag     3060

cggugugaga aauauaagga agaccuuuac cugagauuca cggcucgcac ccucaucgag     3120

gccggagcuc ggagaauuau cgucgaugag acaggccacu uccgcgaccc ugggcggaaa     3180

ggauga                                                                3186


<210>  3823
<211>  3189
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3823
auggccgaga agcagcacag auggggccug gacaucggga ccaacagcau uggaugggcu       60

gugaucgccc ucauugaagg ccggcccgcg gggcuggucg cuacaggauc ccgcaucuuc      120

ucugauggca gaaauccuaa agacgggagc ucccuugccg uugagcggcg cggaccgaga      180

caaaugcggc gcagacggga ucgcuaccug agacggcgcg acagauuuau gcaggcccuc      240

auuaacgugg gccugaugcc cggcgaugcu gccgcucgga aggcccuugu gacggaaaau      300

ccuuaugucc ugcgccagag agggcucgac caggcgcuga cccugcccga guucggacgg      360

gcccucuuuc aucugaacca aagacgcggc uuccagucua aucggaaaac agauagagcu      420

accgccaagg aaagcgggaa agugaagaac gcuaucgccg cguuucgcgc cggaaugggc      480

aaugcucgga caguugggga ggcccuugcu agacgccugg aggacggacg gccugugaga      540

gcccgcaugg ucggccaggg caaggaugaa cacuacgagc ucuauaucgc ccgggaaugg      600

auugcucagg aguucgacgc gcugugggcc ucccaacaga gauuucaugc ugaggugcuu      660

gccgaugccg cucgcgaccg gcugagagcc auccuccugu uccagcggaa acugcucccc      720

guuccugugg ggaagugcuu ucuggaaccg aaccagcccc gcgucgcggc ugcccuuccu      780

ucugcccaaa gauuccggcu gaugcaggag cucaaucacc ugagagugau gacccuugcu      840

gauaaacgcg aacggccccu gagcuuucag gagagaaacg accuccuggc ccaacuggug      900

gcucgcccua aguguggauu cgacaugcuc agaaaaauug ucuuuggcgc caauaaggaa      960

gcguaccggu ucacaaucga guccgagcgc agaaaggaac ugaaagggug cgauaccgcc     1020

gcuaaacuug ccaaaguuaa cgcucuggga acgcgguggc aggcccucag ccuggacgag     1080

caggaucgcc uugugugucu gcuccuggac ggcgaaaaug augcggugcu ggccgacgcu     1140

cucagagagc auuaugggcu gaccgaugcc cagauugaca cacuucuggg acucucuuuc     1200

gaagauggcc acaugcggcu gggccgcucc gcucugcuua gaguccucga cgcccuggag     1260

agcgggcggg augagcaagg acugccccuc ucuuacgaca aggccguggu ugcugcgggc     1320

uauccggccc acaccgcuga ccuggaaaac ggggagcgcg augcccuucc uuacuacgga     1380

gaacugcucu ggagauauac acaggacgcc cccaccgcua aaaaugaugc cgagcggaag     1440

uuuggcaaaa ucgcgaaccc uacggugcau auugggcuga accagcugag aaaacuuguc     1500

aaugcucuca uccagcgcua cggcaagccc gcccaaaucg ugguugaacu ggcccggaac     1560

cugaaggcug gacucgagga gaaggaaaga auuaaaaagc agcagacagc caaucuggag     1620

cgcaacgaac ggaucagaca gaaacuucaa gacgcgggcg ugcccgauaa ucgcgagaac     1680

cggcugagaa ugcgccucuu cgaggaacug gggcagggaa auggccuggg gaccccuugc     1740

auuuauuccg gacggcagau cagccuucag agacucuuuu cuaacgacgu ccaaguggau     1800

cacauucugc cguucuccaa gacacuggac gauagcuuug cuaauaaggu ccucgcccag     1860

caugacgcca accgguacaa aggcaaucgc gggcccuucg aggcuuuugg cgccaacaga     1920

gauggauaug cuugggacga uauccgggcc cgcgcggccg ugcugccuag aaauaagcgg     1980

aacagauucg cugaaaccgc caugcaggac uggcuucaca augagaccga cuuucuggcu     2040

cgccaacuca cagauacggc cuaccugucc cggguugcca gacaguaucu gaccgcuauc     2100

uguucuaaag acgaugugua cgucagcccc ggccgccuua cagcgaugcu ccgggccaag     2160

ugggggcuga acagagugcu ggacggaguu auggaggagc agggccgccc ugcugugaaa     2220

aaccgggaug accacagaca ucacgccauu gaugccgucg ugaucggggc uaccgaccgc     2280

gccaugcucc agcaaguggc gacacuggcu gccagagccc gggagcagga ugcugaacgc     2340

cuuauuggag acaugcccac cccguggccu aauuuccugg aggauguuag agccgcgguc     2400

gcucggugcg ugguguccca uaagcccgac cacggcccug aagggggccu ccauaacgau     2460

acggccuacg gaaucgucgc cggccccuuu gaggacgggc gcuauagagu gcggcacaga     2520

guuagccugu ucgaccugaa gccuggagau cuuucuaaug ugcgcuguga cgcuccccug     2580

caggccgaac ucgagccgau uuuugagcag gaugacgcuc gggccagaga agucgcgcug     2640

acagcccucg cugagcgcua ccggcaaaga aaaguguggc uggaggaacu uauguccguu     2700

cugccuaucc ggccccgcgg cgaagauggg aagacccucc ccgacagcgc cccuuauaag     2760

gcuuacaagg gagauucuaa cuauugcuac gagcuguuca ucaaugagag aggccggugg     2820

gacggggaac ugauuagcac cuuccgcgcc aaccaggccg cuuauagacg guuucgcaau     2880

gauccggcga gauuccggcg cuacacagcc ggcggaagac cccuucugau gcggcucugu     2940

aucaacgacu acauugcugu gggcaccgcc gccgagagaa caaucuuucg cgucgugaaa     3000

auguccgaaa auaagauuac gcuggcugag cacuucgaag ggggaacccu caagcagcgg     3060

gaugccgaca aagacgaucc uuuuaaguau cugacaaaau cucccggcgc gcuuagagac     3120

cugggggcuc gccggaucuu cguugaucuc auuggaagag ugcuggaccc uggcaucaag     3180

ggcgauuga                                                             3189


<210>  3824
<211>  3189
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3824
augaaccuga auuacauccu cggccuggac cuugggauug ccagcguggg augggcuguc       60

guugagaucg augaacagga gaacccccaa agacugauug acgugggcgu gcggaccuuc      120

gaucgcgccg aaguccucaa gacaggggag ucccuggcgc uugcuagacg gcuggcccgc      180

ucuagcagac ggcucgugaa acgcagagcc gaacggauca agaaagcuaa gcgccugcug      240

aaagccgaga aucuccugcu uuccgcugac gagauucugc cuaacgaagc cuggcagcuc      300

agaguuaagg gacuggauca gaagcuggag cggcaggaau gggcggccgu gcugcuccac      360

cuucugaaac aucgcggcua ucucucucaa agaaagaaug agagcaaauc cgagaacaag      420

gaacuggggg cccuucuguc uggagucgcu aucaaucacc agauccucca gagcgcugag      480

uaccggacgc cggccgaaau ugcggugaac aaauuucagg ccgaggaagg ccauauccgc      540

aaucaaagag gcgcuuauac ccacacauuc ucccggcugg accuucuggc cgagauggag      600

cuccuguuuc agagacaggc uacccugggg aacccccaua caagcaccaa gcuccuggaa      660

aaucuuacag cccugcucau guggcagaag ccugcccugg cuggagaggc gauucuuaaa      720

augcugggca agugcaccuu cgaacccacg gaguacaaag ccgcuaagaa cucuuauucc      780

gccgaacgcu uuguuuggcu caccaaacug aauaaccugc ggauccucga gcaaggggcc      840

gagagagcuc ugacagauaa ugaacgcuuc gcccuucugg accagccuua cgagaaggcg      900

aaacucaccu augcucaggu gcggacacug cugggacuua gcgaugaagc cauuuuuaag      960

ggccucagau accaagggga ggacaagaaa gccgucgaaa ccaagacgac acugauggag     1020

gugaaggcuu accaccagau ccgcaaggcc cuggagggac ucgaucugaa agcugaaugg     1080

aacgagcuua agaacaaucc cgcccugcuc gacgaaauug gcaccgcguu cucucuguau     1140

aagacagaug acgacaucca ccgguaccug gauggcaaac uuuccgagag cgugcucaac     1200

gcccugcugg agaaucucaa cuuugacaag uucauccagc ugucucuuaa agcucugcag     1260

caaauucucc cucugaugcu gcaggggcag agauaugaug aagccgucuc cgcuaucuac     1320

ggagaccauu auggcaagaa aagcgaugag auugaccgcc uucucccgaa uauccccacc     1380

gaugaaauuc ggaacccugu ugugcugaga acccugacac aggcccgcaa ggugaucaau     1440

gcggucgugc ggcucuacgg gucccccgcc agaauccaca uugagacggg acgggaaguu     1500

ggcaagucuu aucaagaccg caaaaagcug gagaaacagc aggaggaaaa cagaaagcag     1560

cgggagaaug cugugaaaaa guuuaaggaa uacuucccua acuuugucgg ggagcccaaa     1620

ggaaaggaua uccuuaaaau gagacuguac gaacuccaac aggccaagug ucuguauagc     1680

ggcaaauccc uggagcuuua ccgccuccug gagaagggcu auguggaagu ugaccaugcu     1740

cugccguuca gccggaccug ggaugacucu uucaauaaca aagugcucgu ccuggccaau     1800

gagaaccaga auaaggggaa cgccacaccu uacgaauggc uugacggaaa gaacaauucc     1860

gagggcuggc agcacuuugu ggcuagaguc caaaccagcg gguucucuua ugcgaaaaag     1920

cagcgcgugc ugauucagga acucgaugag aaauccuuua ucgagagaaa ccugaaugac     1980

acacgguacg uugcccgcuu ccugugcaac cacauugcug auaauaugcu ucugaccgga     2040

aagggcaaaa gaaagguguu ugccagcaac gggcaaauca cggcccuccu gcggggcugu     2100

uggggacucc agaagguccg cgaaaauaac gacagacauc acgcucugga ugccgugguu     2160

guggcgugcu cuacaaaaag caugcaggac aagaucacca aauucgucaa guauaaagag     2220

ggccggcuuu uuaaggauaa gaaagacggg guguacaagg gagaacgcau uaauagagag     2280

accggcgaga ucauuucccu gcauuucccc acaccuugga aauuuuucaa ggaaaacgug     2340

gagauccgga uuuuuagcga aaaucccaaa cucgagcugg aaaaccgccu gcccgauuac     2400

ccucaguaua aucacgaguu cguucaaccg cuuuuugucu cuagaaugcc cacccggaag     2460

augacagggc agggacauau ggagacggug aaguccgcua aaagacugga caagggccuc     2520

agcgugcuga aagucccucu cacccagcug aaacuuucug aucuggaacg cauggugaac     2580

cgggagagag aaauggcccu cuacgaggcc cugaaagaau gucuggagca guucgggaau     2640

gaccccgcua aggccuucgc ggagccuuuu uauaaaaagg gcggagcucu uguuaaggcc     2700

gugcgcgucg aacaaacaca gaaauccggc gugcugguuc ggaacgggaa cggaguggcc     2760

gauaaugcua ccggcagcau cgucagagug gacguuuuca caaaaggggg aaaguacuuu     2820

cucgugccca uuuauaccug gcaggucgcc aagggcaucc ugccgaacaa agcugugauc     2880

caggggaagg acgaguccga augggaggaa auggaugaga augccguguu ccaauuuucu     2940

augcacaaga acgaccucau uaaagucaag acgaaaaagg gcgagaucuu cggauacuuu     3000

aauggccugg aucgcgcgac cgggagcauu gaaaucgagg aacacgaccu ugagaaaucc     3060

aagggaaaga acggcucuca ucgggggcug ggaguuaaaa cagccuucuu ucuggagaag     3120

uaucaggugg augaacucgg caaaaauauu agaccuugcc gccccaccaa gcggcagcac     3180

gugagauga                                                             3189


<210>  3825
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3825
auggagaacc acaucaauua cagacugggc cucgacauug ggaucgccag cgugggaugg       60

uccgucauua accuggauaa gaaacggauc gaagaccuug gcguucgcau uuucaaugcu      120

gccgagcauc ccaaggaugg gucuagccug gcgcucccua gacggaucgc ucgcggaaga      180

cggcgccugc uuagacggaa agccuaucgc gugaagagag ugcggaaacu gaucgucgac      240

uacaacauuc ucaccaagga ucagcugaau gaccuguuua agcaaaaaug ccagaacucc      300

gugugggaaa uccgcguuaa ggcccucgau aauaaaguga caaacgagga augggcuaga      360

cugcuuauua aucuguguaa gcggcgcggc uucaaaucua acagaaagaa ugaggccgac      420

gauaaggaga cgaaaaaggu caucaacagc auuaaaucca auaucaaggg gaugaaagaa      480

ucuggaagcc ggaccauugg cgaguauauc uacacacaga cccucuccag cggcgacauc      540

ucuaaguccu uuagaaacaa agaugggcag uauaauaacu gcgugagccg caagaugauu      600

gaagaggaga ucaagauucu guucaaaaag caacgggaac uuggauguga guuugcuucu      660

gaagagaucg aagagaaaua ccuggccauu uucaacuccc agagaccguu uagcaaguuc      720

gaggaccucg aaaagauggu uggcuuuugc acauucgaga aggcgucuaa ggaacgccgg      780

gcccccaaau guuccaucag cgcugaggaa uuugugcugu augauaauau gaacaagcug      840

uccauccuca auaaagggga caccagaaag cugacaaacg aggagcgcaa aauuaucguc      900

gaaaaugccu ucaagaacaa ggagauuaaa uacucuaaac uucggaaaau ccugaaucuc      960

agcaacgacg aaagauuuuc cacccugagc uacucuaaua agacggagcu uuccaaaacc     1020

gaaaacagca aguucguggc ucugaaggga uaucacgaga uuaaaucugc caucgaaaag     1080

agcuucggca aagauacaug gaaucgcgug aagaacaauc ggaccaugcu caacgacauc     1140

gcguacgucc uguccauugc caaaacagau gaagacauca uuaagcagcu gucucucaga     1200

aauaucccuc gcgagauuau cgaugcuguu cuggggcuua gcuuuuccaa auucagcaac     1260

cugucuauua aggccaucga aaagauccuc cccuaucuga aagacggaca gcaauacaac     1320

gaggcuugca aguccgccgg cuaugauuuu aaagccaccu acaauggcga aaagagcuau     1380

aaacuuccuc ugauugacau cgaugaaauu gugaaccccg uggucaaucg ggcucucucu     1440

cagacgagaa aggugaucaa cgcgguuauu gaccgguacg gguccccuau cggaaucaau     1500

auugagcugg cccgcgaucu ggcuaagaac uucaaagaca gaagcaagau caaaaaggaa     1560

caggaggaaa aucgggccaa caaagagaag auuaagaaug agcucaaaau gcugaugaac     1620

aaggaaccga cauccucuga gauccuuaaa uacagacugu ggaaggaaca gcgcgaggaa     1680

ugugccuaua gccaagagcg gauuuacauc gaggaccucu uuaaaguggg ccaguaugaa     1740

aucgaucaua uuauccccuu cuccagaucu uuugacgaua gccugaauaa caagguccug     1800

gugaaggcug ccgagaauca gcgcaaagcg aaccggauuc cuuacgaaua uuucgggucc     1860

gacgaggaua gaugguaccg cuuugaaguu uggguggagc aaagcucucu uuccuucaag     1920

aaaaagaaua accuccugaa aaagagauuu agccgggagg acucuaucga guucaaagau     1980

cgcaaccugg ucgacaccca guauauuucc agauacauca gcaauuuuau caagucucgg     2040

cucauuuuca aggagagcga uuccaaacag aaggugauca caauuaacgg acgcgcuacc     2100

agcuaccuga gagccaaaug gggccuucug aagguccggg aaaaugggga caaacaccau     2160

gcccucgaug cuaccgugau cgccguugcu acacagggaa uggugcaaga gauuucuaag     2220

uauuccaagg cccacgagcu gaaauacauc cgccagggcg acgaguucau ugauaucgag     2280

acgggcgaaa ucgucaaggu gaaagaccac aagcaucuga gcaaagacag acuucccgag     2340

ccuuggcucg gguuuuccuc ugaacugcgg cugagacuca gcgagaaccc cauugaggaa     2400

cugaagaagu ccccgaucuc uaccuaugau gaggaguuca uucgcaaaac aguucggccu     2460

aucuuuguga gcagaguccc cuaccgcaag gugaccggac ggcuuuucga agaaacagug     2520

uauuccccua aagcguuuau ugacgagggc uucaucgcca agaaaaagcu gcaggaucuc     2580

acccugaagg acuuugagaa uaucuacaac uauaauucug auaaaaagcu guacgacgcu     2640

cuuagagaac gccucgagga auacggggga aacgccaaaa aggcuuucgc cgaggaauuu     2700

cggaaaccca cgaagacagg caagcugggg cccauuguua aaagcaucaa ggucaaaacc     2760

aagauuccuu ucaaagaugg caucgaccug aauggaggca aggugaaaaa ggaugggcuc     2820

gugagaauug acaucuauga gaaggaggga aaauacuaug ucgugccggu uuaccgccug     2880

gauauccagc aaggcaacau ucccaagaaa gcggccgugg cuaauaagga agagaaagac     2940

uggauccuua uggaugaauc cuauaacuuu aaguucagcc uguacaagaa ugaccucauu     3000

uauaucaaau acgacucuaa gggggaguac uuuggauauu acgauggcuu cgaccggcac     3060

accggggcca uuacacugga gagacaugau aacuccgaac gguaucgcgg caucggaauu     3120

aaaaccggcg ucaaggagau cuucaaauac aauguggacg uucuggggag auauuacaag     3180

guggcuaagu ga                                                         3192


<210>  3826
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3826
auggagaagu uccacuacgu gcugggccuc gaccugggga ucgccagcgu cggaugggcu       60

gccauugaaa ucgauaaaga gaccgaaaca uccauuggcc uucuggacug cgggguuaga      120

acguuugagc gggcggaagu gcccaagacc ggagauucuc ucgcuaaagc ccgcagagag      180

gcccggagca cacgcagacu gauccggcgc agaucccauc ggcuucugcg ccucaagaga      240

cugcugaaac gggagauuuu ccgccagccu gaaaccuuua aggaccuccc gaucaacgcu      300

uggcagcuga gagugaaggg ccuugauucu cggcugaaug aguaugaaug ggccgcuguc      360

cuccugcacc uugugaaaca ucgcggguac cugagccaga gaaaguccga gaugucugag      420

acagacagca aauccgaaau gggacggcuc cuggccggcg uugcggagaa ccaccagcug      480

cuccagcaag aacaguauag aacccccgcc gagcuggcuc uuaagaaauu cgugaagcau      540

uuucgcaaua agggcgggga uuacgcccac acauucaacc ggcuggaccu ccaggcugaa      600

cugcaccuuc uguuucagaa acaaagagag cucggaaauc cuuucaccag ccccgagcug      660

gaacgccagg ucgaugaccu gcucaugacg cagcggucug cccugcaggg cgaugcgauc      720

cuuaagaugc uggggcauug uggauuugag ccugaacaau ucaaagccgc uaagaacacc      780

uuuuccgccg agagauucau uuggcucaca aaacugaaua accuucgcau ccaggaccag      840

ggcaaggaac gggcucugac cgccgaugag agaacaaaac uccuggacga gcccuauaag      900

aaguccaaac ugaccuacgc ccaagugcgc aagcuccugu cucuuccuca gacggcuauu      960

uuuaaagggc ugcgguauga ccucgaacac gauaagaaag cggagaauag cacacugaug     1020

gaaaugaagu cuuaccauaa caucagacag acccuggaga agucuggacu uaaaacagaa     1080

uggcagucca uugccaccca accggagauc cucgacgcua uuggcaccgc cuucagcauc     1140

uacaagacag augaggacau cucccacgaa cugaaaacgu gccgccugcc cgagaauguu     1200

cucaacgaac ugcuuaagaa uauuaacuuc gauggcuuua uccagcuguc ucucaccgcc     1260

cugcggaaaa uucugccucu uauggagcag ggguauagau acgacgaggc uuguacacag     1320

aucuauggaa aucaccauag cggcucccuc caacaggaaa gcaagcaguu ccugccccac     1380

auuccuaucg augacgugcg gaaccccguc guguuucgca cccugacaca ggccagaaag     1440

guggucaacg cgaucauucg gagauacggg ucuccggcuc gcguucauau cgagauggcc     1500

cgggaacucg gaaaauccaa gagcgauaga gaccgcauug agaaacaaca gcagaagaau     1560

aaaaaggaaa gagagaacgc cguggcuaag uucaaagagg auuuuccuga cuucgugggc     1620

gaaccccggg ggaaggacau ccugaaaaug cgccuuuaug agcagcaaca cggaaagugc     1680

cuguacucug gccaugauau ugacaucaau agacucaacg aaaaaggcua ugucgagauc     1740

gaucacgccc ugccuuuuag ccggaccugg gacgauuccc agaauaacaa ggugcugguu     1800

cuugggucug aaaaucagaa caaacgcaau caaacgcccg acgaguaccu cgauggagcu     1860

aacaauagcc agagauggcu ggaguuccag gcccgggugc agacauguca cuuuuccuac     1920

ggcaagaagc aacgcauuca gcuggcgaaa cucgacgaug aaaccgagaa gggguuccug     1980

gaaagaaacc uuaaugacac ccgguauauc gcccgcuuua ugugccaguu cguccaggag     2040

aaccuguacc ucacaggaaa aggcaagaga cugguguuug cuagcaaugg gggcaugacc     2100

gccacacugc ggaaccuuug gggacucaga aaaguucgcg aagauaacga ccggcaucac     2160

gcucuggaug ccauuguggu cgcguguucu acggccucca ugcaacagaa gaucaccaag     2220

gcuuuccaga gacaugagag cauugaguau guggacacag aaaccggcga ggucaaauuc     2280

cgcauccccc agccuuggga cuuuuuccgg caagaaguga ugauuagagu uuuuagcgau     2340

cagccgugcg aggaccuggu ggagaagcuc uccgcccgcc ccgaagcucu gcacgauaau     2400

gucacaccuc uuuucgugag ccgggccccc aacagaaaaa uguccgggca gggacaccug     2460

gagaccauca agucugccaa acgccucagc gaagaaaauu ccaugguuaa gaagccucug     2520

acgacccuga aacuuaagga cauccccgag auugugggcu acccgucucg ggagccucaa     2580

cuguaugcug cgcucaaaac aagacuggag acccaugaug acgaucccau caaggccuuu     2640

gcuaaaccuu ucuacaagcc caacaagaau ggggaacucg gagcccuggu ccggagcgug     2700

cgcgugaaag gcguucagaa cacagggguc auggugcacg acggcaaggg aauugccgau     2760

aaugcuacca uggugagagu cgacguguau acgaaagccg gcaagaacua ccuuguuccu     2820

guguacgucu ggcaaguggc gcaggggauc cugcccaauc gggcuguuac auccggaaaa     2880

agcgaggccg auugggaccu cauugaugaa ucuuuugagu ucaaguuuuc ccugagccgc     2940

ggcgaccugg uggaaaugau cucuaacaaa gggagaaucu ucggauauua caauggccuu     3000

gaccgggcca acggguccau uggcaucaga gagcaugauc uggaaaagag caagggaaaa     3060

gacggcgucc accgcguggg gguuaagacc gcuacagccu uuaauaaaua ucauguggau     3120

ccgcucggaa aggagauuca ccgguguucu agcgagccua gacccacccu gaaaaucaag     3180

uccaagaaau ga                                                         3192


<210>  3827
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3827
auggagaagg acuacauccu gggccucgau auugggaucg gaagcguggg cuaugggcug       60

auugacuacg auaccaaauc caucauugac gccggaguca gacuuuuccc cgaagccaac      120

gccgauaaua accugggccg gcgcgcgaag agaggggcuc ggcgccucaa aagacggcgc      180

auccacagac uggagcgggu uaagucucuu cugagcgaau auaaaaucau uuccggacuc      240

gccccuacaa auaaccagcc guacaauauc cgcgugaagg gccugacgga gcaacugacc      300

aaggacgaac ucgccguggc ucugcuucau auugccaaaa gacggggcau ccacaacguc      360

gauguggcug ccgacaagga ggagacagcg ucugauagcc uguccaccaa agaccagauu      420

aauaagaacg ccaaauuucu cgaaucucgc uauguuugcg agcugcagaa ggaaagacuu      480

gagaaugagg ggcaugugcg gggagucgaa aaccgcuucc ugacaaagga uaucgugaga      540

gaggcuaaaa agauuaucga cacccagaug caauacuauc ccgaaaucga ugagacauuu      600

aaagaaaagu acauuagccu cguugagacc cggagagagu acuaugaagg cccugggaaa      660

ggaucccccu acggcuggga cgccgacgug aagaaauggu aucagcugau gauggggcac      720

uguacguacu ucccugucga guuucgcagc gugaaguaug cuuacaccgc cgaucuguau      780

aaugcgcuca acgaccugaa uaaccuuaca aucgcccggg augacaaucc caagcuggaa      840

uaccaugaga aauaccacau uaucgaaaac guguucaagc agaaaagaaa cccuacccuc      900

aagcagauug cuaaagagau cggagucaau gauaucaaca uuucuggcua ucgcguuaca      960

aaguccggca agccgcaauu uaccagcuuc aaacuguuuc acgaccuuaa gaaaguggug     1020

aaggaucaug ccauccugga cgauauugac cuccugaauc agaucgcuga gauucugacg     1080

aucuaccagg auaaagacuc uaucgucgcc gaacucgggc agcuggagua ucuuaugucc     1140

gaagccgaca agcaaagcau uucugagcug acaggauaca ccggcacaca cucccucagc     1200

cugaagugca ugaacaugau cauugaugaa cuuuggcauu ccucuaugaa ucagauggag     1260

guguucaccu aucugaacau gcggcccaaa aaguacgagc ucaaagggua ucagagaauc     1320

ccuaccgaca ugauugauga cgcuauccug agccccguug ugaagcgcuc cuuuaaacaa     1380

gcgauuggag ucgugaaugc caucaucaag aaguacggcc ugccuaaaga uauuaucauu     1440

gaacucgcuc gggagagcaa cucugccgaa aaguccagau accugcgcgc cauccagaaa     1500

aagaaugaga aaacacggga gagaauugaa gcuaucauca aggaguaugg gaacgaaaau     1560

gccaaaggac uuguucagaa gauuaagcug cacgacgcgc aggagggcaa augucucuac     1620

agccugaagg auaucccccu ggaagaccuu cuccgcaacc cgaauaacua ugauauugac     1680

cacaucauuc cucggucugu gagcuucgau gacuccaugc auaauaaagu ccuggugaga     1740

cgggagcaaa acgcuaagaa aaacaaucag acgcccuacc aguaucugac cucuggcuac     1800

gccgauauca aguauagcgu cuuuaagcag cacgugcuca accuggccga gaauaaagac     1860

cgcaugacaa agaaaaagag agaauaccuu cuggaggaac ggaacaucaa uaaauacgac     1920

guucaaaagg aguucauuaa cagaaaucuc guggauaccc gcuauacaac ccgggaacug     1980

acgacacugc uuaagaccua cuucaccauc aacaaucucg acgucaaagu gaagacaauu     2040

aacggguccu uuaccgauuu ccugagaaaa cgcuggggau uuaagaaaaa uagagacgag     2100

ggcuauaagc aucacgcuga ggaugcccug aucauugcua acgccgacua ccucuucaag     2160

gaacauaaac ugcuuaagga gaucaaagau guuagcgacc uggcggggga ugaacgcaau     2220

ucuaacguga aggacgagga ucaguaugaa gaggucuuug gaggcuacuu caaaaucgag     2280

gacauuaaga aauauaagau caagaaauuu ucccaccggg uggauaagaa accuaauaga     2340

cagcucauua acgacacaau cuacagcacg cgggugaagg acgauaaacg cuaccugauu     2400

aacacccuga agaaucuuua ugacaagucu aacggggauc ucaaagaaag aaugcagaag     2460

gaccccgagu cccugcugau guaccaccau gauccccaaa cauucgaaaa acucaagauc     2520

guuaugagcc aguaugagaa ugagaaaaac ccucuggcca aguacuuuga agagaccggc     2580

caguaucuua caaaguacgc uaaacacgac aauggaccgg ccauucauaa gaucaaauau     2640

uacggcaaca agcuggucga acaccucgau aucaccaaaa auuaccacaa cccccagaau     2700

aagguggugc aacuguccca gaagucuuuc cgguuugacg ucuaucagac ggauaaaggg     2760

uacaaguuca uuagcaucgc uuaucugacc cuuaaaaacg agaagaauua cuaugccauu     2820

ucccaagaaa aauacgacca gcucaagucu gagaagaaaa ucagcaacaa ugcgguguuu     2880

auuggauccu ucuauacaag cgauaucauc gagauuaaca augaaaaguu ccgcguuauc     2940

ggcgugaacu cugacaaaaa caaucugauu gaggucgaca gaaucgauau ucggcagaag     3000

gaauuuaucg agcuggaaga ggagaaacag gugaacuaca ugaacuccga cagcuaccuc     3060

aagaauaauc gcaucaaagu uaccauuggg agaaagacaa ccaacaucga aaaguuccau     3120

acggauauuc ugggaaauau guauaaaucu aagcggccua aagcucccca acuuguguuu     3180

aagaaaggcu ga                                                         3192


<210>  3828
<211>  3195
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3828
augagcgagu gcgugaccag acggauccug ggcauugacc ucgggaucgc cuccugugga       60

uggggcguca uugaaguugg ggaggcuucu ggaagcauca uugccuccgg cgugcgcugc      120

uucgaugcgc cccugaucga caagacaggg gaaccuaaau cugcuacgag acggaccgcc      180

cgcggacaga gacggaucau ucgcagacgg cgccaaagaa ugaacgccgu gcggcgccuu      240

cuggcugagu uuggcguccu cacaggcaga agcccggaug cccugcacca ggcccuucug      300

cggcucuccc agucuguggc ugggagccag guuacccccu ggacacugcg cgcggccgcu      360

caugaaagaa agcugaccaa ugacgagcuc gccguggucc ugggacacau cgcucggcau      420

agaggcuucc gcuccaacag caaaaaugau gggggagcca acgcggccga cgagacaucu      480

aagaugaaaa aggcuaugga aaccacgcgg gagggccuug ccagauacca cuccuuuggg      540

gcuaugauug ccagcgaucc uaaguucgcc gaccgcaaac ggaauagaga uaaggacuau      600

ucucauaccg cuaaacgcuc cgaucuggaa gacgaggugc ggacaaucuu uagaagccaa      660

acccgcuucg gaucucucgu ugcguccgag aagcugagcc aggccuuugc ugaugccgcc      720

uucuuucagc ggccccuuca ggacuccgaa gacauggugg gcucuugucc uuucgagccc      780

ggccagaaaa gaacagcucg ccgggccccu agcuuugaac uguucagauu ucucucccgg      840

cuggcgaacc ugaagcucac cgucgggcgc gcuccggaga gacggcugac gcccgaugaa      900

auugcccuug ccgcuaaggg auucggcgag accaaaaaga gcaucacauu caaaucucug      960

agagaggccc ucgaccugga uccuaaugcu cgcuuuuccg ggguggccaa ggaaaaagag     1020

agcacacuug acguggcggc ccggaccgga ggcgcugccu acgggaccaa gacacugaaa     1080

gaugcucucg gagacgcccc cuggagaucu cugagccgca ugccugaaaa gcuggauaga     1140

auugcggaga uccucuccuu ccgggaagac augaaggcca uccgcaacgg ccuggaggag     1200

gucggccuug augggcuggu uguggacgcu cucaugcaag ccacggcuaa uggagauuuu     1260

aaagacuuca ccagagccgc ccacauuucu gcucuggcgg cccggaacau cauucccggc     1320

cuucgcgaag ggcuggugua uagcgacgcu ugcacaagag ucggauacga ucacgccgcc     1380

cggccggcug ugccucucuc ccagaucggc agccccguua cccgcaaggc ccugucugag     1440

gcgcugaaac aggugagagc ugucgcccgg gaauaugggc cuauugacua cuuucauauc     1500

gagcucgccc gcuccaucgg caagagcgcu gaagagagaa agaaacugac agauggaauu     1560

gaggcccgga auguggaaaa ggagaaaaga cgcaaggaag cggcugagca ccuuggccgg     1620

gcccccucug acgaugagcu gcucagauau gaacuggcca aagagcaaaa cuucaagugu     1680

aucuacuccg gggaccccau ugauccugcu ggaaucagcg ccaaugacac ccgcuaccag     1740

guugaucaua uucugccgug gucccgguuu ggcgacgauu cuuaugugaa caaaacgcuu     1800

ugcacagcua gaagcaauca gaacaagcgc gggcggaccc ccuucgaaug guuugacgcc     1860

gauaagaccg aggcggaaug gauggaguac uccgccagag ucgaggaccu caaagaagug     1920

aagggacgca aaaagcggaa cuauucuauc aaagacgcug ccagcgucga ggauaaguuc     1980

aaggcuagaa aucugacaga cacccaaugg gccacacggc ugcucgccga ugaacugaaa     2040

cgcauguuuc cucccagaga gugugaacgg gugguuacgg ugcgcgcuga cggcgggaac     2100

gauggccuuu ccaucgucga ggagagacgg guguucacca gaccuggagc gauuacaagc     2160

aagcugcgcc gggccugggg ccucgaaggg cugaaaaagc aggacggaaa aagaguugag     2220

gaugaccgcc accacgcugu ggaugcccug guccuugccg cuaccacaga aucucuccug     2280

aaucggcuga ccguggaggu gcagcagaga gaacgcgagg gccggcaaga cgauaucuuu     2340

cauugcuccc agcccuggcc gggguucaga guugacgucc agcgcacggu guacggaagc     2400

gagaccaugc cuggcauuuu cgugucuaga gccgaacggc gcagagcgcg ggggaaggcu     2460

cacgaugcca cagucaaaca gauccgcgac auugacggcg agagaaucgu guuugaacgg     2520

aagcccauug agaagcucac cgauaaagac cuggagagaa ucccuguucc cgaaccuuau     2580

ggaaaggccg cugaucccaa aaagcugcgc gacgagcuug uggaaaaccu ccgggccugg     2640

aucgcggcug gcaaaccgaa ggauaagccu cccagauccc ccaaagggga cauuauccgc     2700

aagguccgga uugagacaaa agauaaggug gccguugaaa ucaauggagg caccguggac     2760

agaggggaua uggcccgggu cgacguguuc cgcaaaaaga acaagaaagg aguuugggag     2820

uuuuacguga uuccuaucua uccgcaucaa aucgucgcua gcgcccugcc cccuaauaga     2880

gcugugauug ccuacaaggc ggagucugaa uggacggcca ucgauggcug uuucgaguuu     2940

gcuuggagcc ugaaccccau guccuaccuu gaacucguga aaagcaaugg ggagcugauu     3000

gaaggcuauu uccggucuau ggaccgcaca accggagcca ucaaccuguc cccuaugagc     3060

acaaauuccg agaccauuag aucuaucggc gucaagaccc ucagcuccuu ucggaaauuc     3120

acaguugacc gccuggggag aaaguuugag aucccccggg aagugcgcac guggagagga     3180

gaggcuugca ccuga                                                      3195


<210>  3829
<211>  3195
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3829
augaagagaa ccagccugcg ggccuaccgc cucggcgugg accugggggc uaacucccuu       60

ggaugguucg ucguuuggcu ggaugaccac ggccagcccg aggggcucgg accuggcggg      120

gugagaaucu uuccggaugg acggaauccc caaucuaaac agagcaacgc cgcgggccgc      180

agacuggcuc gguccgcccg cagacggcgc gacagauauc uucagcggcg cggcaagcug      240

auggggcucc uggugaaaca uggacugaug ccugccgaug aacccgcuag aaagcggcuc      300

gagugccugg acccuuacgg ccuucgcgcc aaagcucugg augaaguccu cccccugcac      360

caugugggga gagcccuuuu ccaccugaau cagcggagag gacucuuugc gaaccgcgcc      420

auugagcaag gcgacaagga ugcuucugcc aucaaggcug ccgcggggcg gcugcagaca      480

agcaugcagg ccuguggagc uagaacgcug ggcgaauuuc ucaaucgccg gcaucagcug      540

agagccaccg uucgcgcucg guccccugug ggcggggacg uccaagccag auaugaguuu      600

uacccgacac gcgccauggu ggaugcugag uucgaagcga uuugggccgc ucaggcuccc      660

caccacccua ccaugacagc cgaggcucau gacaccaucc gggaagccau uuucucucag      720

agagcgauga aacgccccag caucggaaag ugcucccuug auccugcuac aagccaggac      780

gacguugaug gcuuccggug ugccuggucu cacccccugg cccaaagauu ucggaucugg      840

caggacgugc gcaaccucgc ugucguggag accgggccga cguccagcag acugggaaaa      900

gaggaucagg acaagguggc ccgggcccuu cugcaaaccg aucagcucuc uuucgacgaa      960

auuagaggcc ugcuggggcu cccuuccgau gcccgcuuua aucuggagag cgaccggaga     1020

gaucaucuua aaggagacgc gacaggcgcc auccugucug cucgcagaca cuucggcccc     1080

gccuggcacg aucggucccu cgaccgccag auugacaucg ucgcucugcu ugaaagcgcc     1140

cuggaugagg cggccauuau cgcuucccuc gggaccacac auucucugga cgaagccgcu     1200

gcccagagag cccugagcgc ucuccugccu gauggauauu gccggcuugg ccugcgcgcg     1260

auuaagagag uucucccccu gauggaggcc gggcggaccu acgcugaggc cgccuccgcu     1320

gccggauaug accacgcgcu gcuucccggc gggaagcuca gcccuacggg cuaccugccg     1380

uacuauggac aauggcugca gaacgaugug gugggcucug acgaugaacg cgacacaaau     1440

gagagacggu gggggcgccu ccccaacccu accguccaua ucggaaucgg ccagcugaga     1500

cgggugguua augaacuuau uagauggcac gggcccccug cugagaucac aguggaacug     1560

acccgcgauc ucaaacuguc ccccagaaga cuggccgagc uugagcgcga acaggccgag     1620

aaccaacgga agaaugacaa aagaaccagc cuccugcgca agcugggacu cccggcuucu     1680

acacauaacc ugcuuaaacu gcggcucugg gaugaacagg gcgacgucgc cagcgagugu     1740

ccuuacacgg gggaagcgau uggccuggag agacuggugu ccgaugacgu ugacaucgau     1800

caccuuauuc ccuuuucuau cagcugggac gauuccgcug ccaauaaggu ggucugcaug     1860

cgcuaugcca accgggagaa aggaaacaga accccuuucg aggcuuucgg ccaccggcag     1920

gggcgccccu acgacugggc cgauaucgcu gaaagagccg cgcggcuccc ucgcggaaag     1980

agauggcggu uuggccccgg ggccagagcu caguucgagg aacugggaga cuuucaagcc     2040

cgccugcuca augagacaag cuggcuggcu cggguggcca agcaguaucu ugccgcuguc     2100

acccauccgc accggauuca ugugcugccu ggccgccuca cagcgcugcu gcgggccacc     2160

ugggaacuua acgaucuccu gcccgggucu gacgauagag cugccaaauc ccgcaaggac     2220

caccggcacc augccaucga ugcucugguu gccgcgcuca cggaccaggc ucugcuuaga     2280

cgcauggcca augcccacga ugacacaaga cggaaaauug aggugcugcu ccccuggccu     2340

accuuccgca ucgaccugga aaccagacug aaggcuaugc uugucagcca uaaaccggau     2400

cacggccugc aggcccggcu ccaugaggac acagcguacg gaaccgugga acaccccgag     2460

acagaggaug gcgcuaaccu gguuuaucgc aagacguuug uggacauuuc ugaaaaggag     2520

aucgauagaa uccgggacag acgccuccgg gaucugguca gagcccacgu ggccggggaa     2580

cgccaacagg gaaaaacccu uaaggcugcc gugcuguccu ucgcucagcg gagagacauu     2640

gccggccauc cuaaugggau ccggcacguu cgccucacaa aaagcauuaa gcccgauuac     2700

cuggucccua ucagagacaa agcgggacgg auuuacaagu ccuauaacgc cggcgagaau     2760

gcuuuugugg auauccugca agccgaaucu gggcgcugga uugcuagagc caccacagug     2820

uuccaggcca accaggcuaa ugagagccau gacgcgcccg ccgcucagcc uaucaugcgg     2880

gucuuuaagg gcgacaugcu ucgcaucgau cacgccggag ccgagaaauu cgugaagauu     2940

guuagacugu cccccucuaa caaucuccug uaccucgugg aacaucacca agcuggcguc     3000

uuucagaccc ggcacgacga uccggaggac agcuuccgcu ggcuguuugc cuccuucgau     3060

aaacuuagag aauggaacgc ggagcuggug cggaucgaca cgcucgggca gccuuggaga     3120

cgcaagcggg gacuggaaac cggcagcgag gaugcuacaa gaauugggug gacccgcccc     3180

aaaaaguggc cuuga                                                      3195


<210>  3830
<211>  3198
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3830
augaacaagg acaucaauua cgcccugggc cucgauauug ggaucaccag cgugggaugg       60

gcugucauua accuggaccu uaauagaauc gaggaucugg gcguucggau uuucaacgcc      120

gcggaaaauc ucaaagacgg guccucucug gcccuucccc gcagacuggc ucggggacgc      180

agacggcucc ugcgcagaaa ggccuaucgg guggagcgcg ugagaaaacu gauccucaag      240

aacaauaucc ugacaaaaga ggagcuugaa ggccuguuua agaacaagga ggucauugau      300

gugugggagg cucggguuaa agggcucgac uacaagcuga gccgcgaaga gugggccaaa      360

auccuuauua auuucugcaa gagacgggga uuuaaaucca accgcaagaa ugaagcuaag      420

gauaaagagg ccggccagau ccugucuagc auuuccuaua acaucgagaa gaugaaagaa      480

acgaauagca gaaccauugg cgaguacauc uauaacgaag ugaagaauag cgaggacucc      540

uacgcgccuc uccggaacaa auucggggaa uauaagaugu gugucagcag agaugugauc      600

cgcgaggaga uucacacacu guuugaaaaa caacgggagc ugggaaacaa uuucgccucu      660

gacgaaaucg aggauaagua cuuugaaauu uucaacuccc agagaccguu uagcaauuuc      720

gaggaccucg agaagcuggu uggcuuuugc accuucgaac gcaaaaagca uaaacgggcu      780

cccaagcacu guaucucuuc cgaggaauuu acacuuuacg aggggauuaa caaacugagc      840

aucaucaagg auggagaaaa gagaaaacuc ucugaggagg aacgcacccu gguggucaau      900

gaggccuuca acaagaaaga aauuaaguau acaacccuuc ggaaacugcu cggccugucc      960

gaggaggaac uguucagcac gcucaccuac uccaucgaca aggacauuuc uaagacagag     1020

aauaccaaau uugugagccu gaagggguau caugaaauca aaagagcuau ugagaaggga     1080

guguccaaaa aggccuggca ggauaucgaa aacaaucgca accuucugaa ugacaucgcg     1140

uacguccuca cacugggcaa gaccgaugac gagauugaga aacagcuucg gcugagaaac     1200

guuccugaag aacucuucga aagccugcug gauaugucuu uuaauaaguu caacaaucuc     1260

uccaucuaug cccuggagaa aauucuuccc uuuaugaagg aaggcuacca auauaacgag     1320

gcuugcgaga aagccgggua caacuucaag gcuaucuacg aaggagugcg cacgaaaaag     1380

cugccuguga uugagaucga cgaaauuguc aaucccgugg uuaaccgggc ccucgcccag     1440

acaagaaagg ugaucaauag cgucaucgau aaauauggcu cuccugugcg gauuaacauc     1500

gagcuggcuc gcgaccuggc gaagaauuuu aaagauagaa aacuuauuga aaaggagcag     1560

aaggagaacc gggccaaucu cgacaagauc agagaaaacc ugcgcgagcu gauggauaaa     1620

gaaccgaccg uuguggaggu ccucaaguac cggcuguggg agcagcaaag aggggaaugu     1680

gcuuauacac agagccagau uccccuugag cgccuguucu ccccuggaua cugcgaaauc     1740

gaccacauca uucccuuuuc uagaucuuuc gacgauuccc ucagcaauaa aauccuggug     1800

cugggcucug agaaccagcg gaaggggaau cgcaccccuu augaauacuu uggagaggac     1860

uccgagagau ggaacagcuu cgaaauuugg gucaaaggcu cuuaucuuaa uuacaagaag     1920

aaagccaacc uccugaagaa aaaguuuucc gaggaggaac aacgggauug gaaagcccgc     1980

aaucugcagg acaccaagua caucuguaga uauauuagca acuucgugaa caaucggcuc     2040

gaguucaagg aauccgagaa caaacagaag guuaucacag ugaauggccg cgcuacggcc     2100

uaccugagau cucggugggg gcuuaacaaa guccgcgaag auggagacaa gcaucacgcg     2160

cuggaugcug ccgugguugg cgugaccaca caagggaugg uccagaaaau cagcaaguau     2220

uccaaagccc acgagcucua caccgugaga aagaaugacg aauuugugga uguugagaca     2280

ggagaaugcg ucgacgugga ugaguauaag gagauucgga aagacauccu gcccagaccg     2340

uggcgcggcu ucucugagga acugaaacuu cggcucagcg auaacccucu ggaggaacug     2400

aagaaguccc ccauugagac cuacaaugaa gaguuuauca aagaaaacgu gaagccuauu     2460

uucgucagca gagugcccuu ucgcaagauc ucugggaaac ucuucaagga gacgaucuau     2520

uccgaacggg cuuuuaaaga cggcuacuuc guuagcaaga aaucucuggu ggaccuuaag     2580

agaggagauc uggacaauuu uuacaacuau gagugugaua agaaacucua cgacgccauu     2640

uccgagcgca ugaaggaguu caauuauaac ggcaaaaagg cuuuugaaag ccccuuccgg     2700

aaaccuacaa agucugggaa ggaaggaccg gucgugagaa gcguuaaaau caaguccgcc     2760

gugcccuuua aagauggcau uccuaugaau gaggggcugg ucgcgaagga aggaauggug     2820

cgcaucgaca uuuacgagaa agagggcaag uauuucguug ugcccgucua ccgguaucag     2880

cuggccaagg ggaucgugcc uaaaagagcu gccauugcua agaaagauga acuuaagugg     2940

cccaucaugg accagaccua caacuucaaa uuuagcaucu acaagaauga ucucauugag     3000

gugaaauaug acaagaaggg cgucuacuuc ggauauuacg auggcuuuga ccggaacucu     3060

gccucccuga ccaucgaaaa acaugauaac agcgagcgcu auagauccau ugggcugaag     3120

guuggagugc ucgaaaucaa uaaauacgag guggacgucc ugggcaacua ucacaaggug     3180

aaacuugggg gaaauuga                                                   3198


<210>  3831
<211>  3198
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3831
augaacaagg acaucaauua cgcccugggc cucgauauug ggaucaccag cgugggaugg       60

gcugucauua accuggaccu uaauagaauc gaggaucugg gcguucggau uuucaacgcc      120

gcggaaaauc ccaaagacgg guccucucuc gcucugccuc gcagacuugc ccggggacgc      180

agacggcugc uccgcagaaa ggccuaucgg guggagcgcg ugagaaaacu gaucgucaag      240

aacaauaucc ugacaaaaga agaacucgaa ggccuguuua agaacaagga ggugauugau      300

guuugggagg cucgggugaa agggcuugac uacaagcuga gccgcgaaga gugggccaaa      360

auccucauua auuucugcaa gagacgggga uuuaaaucca accgcaagaa ugaaacgaag      420

gauaaagagg cuggccagau ccugucuagc auuuccuaua acaucgagaa gaugaaagaa      480

accaauucua gaacaauugg cgaguacauc uauaacgaag ucaagaauag cgacgauucc      540

uacgccccgc uucggaacaa auucggggag uauaagaugu gugugagcag agacguucug      600

cgcgaagaga uccacacccu cuuugagaaa caacgggaac ugggaaacaa uuucgcgucu      660

gaugagauug aagacaagua cuuugagauc uucaacuccc agagacccuu uagcaauuuc      720

gaagaucugg agaagcucgu gggcuuuugc acauucgagc gcaaaaagca uaaacgggcc      780

ccuaagcacu guauuucuuc cgaagaguuu acccuguacg aagggaucaa caaacuuagc      840

auuaucaagg acggagagaa gagaaaacug ucugaagagg agcgcacacu cgucgugaau      900

gaggcuuuca acaagaaaga gaucaaguau accacgcugc ggaaacuucu gggccucucc      960

gaagacgagc uguucagcac ccugacauac uccauugaua aggacaucuc uaagaccgag     1020

aauacaaaau uugugagccu caagggguau caugaaauua aaagagccau cgagaaggga     1080

gucuccaaaa aggcuuggca ggauauugaa aacaaucgca accugcuuaa ugacaucgcc     1140

uacguucuga cccucggcaa gacggaugac gagaucgaaa aacagcugcg gcuuagaaac     1200

gugcccgagg agcuguucga aagccuccug gauaugucuu uuuccaaguu caauaaccug     1260

agcauuuaug cgcucgagaa aauccugccu uuuaugaagg aaggcuacca auauaaugag     1320

gccugcgaaa aagcugggua caacuucaag gccauuuacg agggagugcg cacaaagaaa     1380

cuucccguca ucgagauuga cgaaaucgug aacccuguug ugaaucgggc ucuggcccag     1440

accagaaagg ucauuaacuc ugugaucgau aaauauggca gcccgguucg gaucaauauu     1500

gagcucgccc gcgaccuggc uaagaacuuu aaagauagaa agcugaucga aaaagagcag     1560

aaggaaaauc gggcgaaccu ugacaagauu agagagaauc uccgcgagcu gauggacaaa     1620

gaacccacag ccguggaggu ccugaaguac cggcucuggg aacagcaaag aggggagugu     1680

gcuuauaccc agucccagau cgcccuggag cgccuuuucu cuccuggaua cugcgaaauu     1740

gaucacauca uccccuuuag cagauccuuc gacgauagcc ugucuaacaa aauucucgug     1800

cugggcuccg agaaucagcg gaaggggaac aaaaucccuu augaauacuu uggagaggac     1860

agcgaacgcu ggaacucuuu cgagauuugg gucaagggcu ccuaucugaa uuacaagaaa     1920

aaggccaauc uucucaaaaa gaaauuuacc gaggaagaac aaagagauug gaaggcucgg     1980

aaccugcagg acacaaagua caucugucgc uauauuagca auuucgugaa caacagacug     2040

gaguucaaag aguccgaaaa uaagcagaaa guuaucacgg ugaacggccg ggccaccgcg     2100

uaccuccgcu cuagaugggg gcugaauaag guccgggagg auggagacaa acaucacgcu     2160

cuugaugccg ccgugguugg cgugacaacc caagggaugg uccagaagau cagcaaauau     2220

uccaaggcuc acgagcugua cacagugcgc aagaacgacg aauuugugga uguugagacc     2280

ggagaaugcg ucgacgugga ugaguauaaa gaaauuagaa aggacauccu cccccggccg     2340

uggagaggcu ucucugagga gcugaaacug cgccuuagcg acaauccucu cgaggaacug     2400

aagaaacugc ccauugaaac guacaacgag gaguuuauca aggaaaaugu gaagccuauu     2460

uucgucuccc gggugcccuu uagaaaaauc agcgggaaac ucuucaagga gacaaucuau     2520

ucugaacgcg ccuuuaagga uggcuacuuc guuuccaaaa agagccuggu ggaccuuaag     2580

cggggagauc uggacaacuu uuacaauuau gagugugaua aaaagcucua cgacgcuauu     2640

ucugaaagaa ugaaagaguu caacuauaau ggcaagaaag ccuuugaguc ccccuuccgc     2700

aagccuacca agagcgggaa agaaggaccg gucgugcggu cuguuaagau caaaagcgcg     2760

gcccccuuua aggauggcau uccuaugaac gaggggcugg uggcuaaaga aggaaugguc     2820

agaaucgaca uuuacgagaa ggaaggcaag uauuucgugg uucccgugua ccgcuaucag     2880

cuggccaaag ggaucguccc uaagcgggcu gccauugcgg gcaaauccga gcuuaagugg     2940

cccaucaugg aucagaccua caauuucaaa uuuagcaucu ucaagaacga ccucauugag     3000

aucaaauacg ccaagaagga uggauauuuu ggcuacuaug acggguucga uagacauaca     3060

gcuucucugg ugauugaaaa ucacgacaac uccgaccggu accgcggaau cggcgugaaa     3120

accggggucg uugaguuuaa caaguaugaa guggaugugc ugggaaauua ccauaaaguc     3180

aaccucggcg ggaauuga                                                   3198


<210>  3832
<211>  3201
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3832
augagcgaga gagugguccg gcgcauccug ggcauugacc ucgggaucgc cuccugcgga       60

uggggcguug uggauauuuc uggggcugga ggcgggauca uugccaccgg agugagaugu      120

uucgacgcgc cccugaucga uaagacaggc gaaccuaaaa gcgcuacgcg gcgcaccgcc      180

agaggccagc ggcgcaucgu cagacggcgc agacaacgga ugaacggggu gcgcagacuu      240

cugugcgagu uuggacuccu gccggacccc cggccugaug cccuuaauca ggcuaugcgc      300

agaauuucca cagccucugc ugccgcgcag guuacccccu ggacacugcg ggccgcugcc      360

caccagagac uccugagcaa cgaagaacug gcuguggucc ucggccauau cgcccgccac      420

cggggguuca gauccaaugc gaagaacgaa gccggagcua augccgcuga cgagaccucu      480

aaaaugaaga aagccaugga ggccacacgc gaaggccugg cuaaguacca ugcguuuggg      540

gauaugauug ccaacgaccc uaaguucgcu aaucggaaaa gaaaccgcga uaaggacuau      600

agccacaccg ccaaacgguc cgaucuugag gacgaaguga gagccauccu gcgcgcucaa      660

cuccgguuug gaagcgccgc ggcuacggag acccuggccc agacauucgc cgauguugcu      720

uucuuccaga gaccccuuca ggacucugag gaccgcgugg gcgauugucc uuuugaaccg      780

ggccaaaagc gggccgcuag acgggccccc uccuucgagc uguuucgcuu ccucagcaga      840

cuggcgaauc ugaaacucgc cgucgggcgg ucuccugaaa gacgccugac cgcugaggaa      900

auugcccuug cugccaaggg auuuggcgag acaaagaaaa ccaucacguu caagucccug      960

cgggaggcgc ucgaccugga ucccaacgcc agauucagcg ggauuggaaa agacaaggag     1020

gcuucucuug augugguggc ccgcacaggc ggggcugccu acggaaccaa aacacugaag     1080

gacgcccucg gcgaugcucc uuggagaucc cugagccgga cccccgagac ccuggaccgc     1140

aucgcggaaa uccucuccuu uagagaggau auuacaucua uccggaaugg ccuggaagac     1200

cuugggcugg auagccucgu cguugaggcc cugaugcagg cugccgccaa cggagacuuc     1260

aaagaguuua cgcgcgcugg ccauauuucc gccagagcgg cucggaauau cauuccgggg     1320

cuucgcgaag gacuggugua uagcgaggcc ugcgccagag ugggcuacga ccacgcugcc     1380

cggcucucug ucccucugga ucaggugggg ucccccguua cccgcaaggc gcugagcgaa     1440

gcucucaaac aggugagagc cgucgcccgg gaguauggcc cuaucgacua cuuccacauc     1500

gaacuggcua gaucuguggg aaaaagcgcc gaggagcgca agcaacuuac agauggcauu     1560

gaagcucgga acguugagaa agccaagaga cgcaaacagg cggaagaaca ucuggggcgg     1620

gcccccuccg acgaugagcu ccugagauau gaacuggcua aggagcagaa uuuuaagugu     1680

aucuacucug gagacgccau ugaucccgcu ggcguggccg ccaacgacac ccgcuaccaa     1740

gucgaucaca uccuuccuug gagccgguuc ggggacgauu ccuaucucaa caaaacacug     1800

ugcaccgcua gaagcaauca gaacaagcgc ggacggacgc cguuugaaug guucgaggcg     1860

gacaaaacag auguggaaug gauggaguac gucgccagag uggagaaucu gcaggaaguu     1920

aagggccgga aaaagcgcaa cuauucuauu aaggacgcug ccgccaucga ggacaaauuu     1980

aaggcuagaa aucucaccga uacccagugg gccacacggc ugcuugcgga cgaacugucc     2040

cgcauguuuc ccccuagaga gugugaacgg gcuaucugcg ggagagccga uggcggaaac     2100

gacggccuca ccauugugga ggagcgccgg gucuuuacaa gacccggggc caucacgagc     2160

aaacugcgcc gggcuugggg acuggaaggc cuuaagaaac aagaugggaa gagaguggag     2220

gacgaucgcc aucacgccgu ugacgcgcuc gugcuggcug ccaccacaga aucucugcuc     2280

cagcggcuga ccgucgaggu gcagagacgc gaaagagagg gacggccuga ugacauuuuc     2340

cacugugccg agcccuggcg cggcuuuaga gcugaugugc ggcgcacagu uuacgggucc     2400

gaaaccaugc cgggcaucuu cgucagcaga gccgagcgga gacgcgcucg gggaaaggcc     2460

caugacgcga cgauuaaaca gaucagagaa auugagggcg agcgccuugu guucgaacgg     2520

aagccugugg agaaacugac cgauaaggac cucgaaaaga uccccauccc uaagcccuau     2580

gggcaagucu ccgacccuaa aagacugcgg gaugagcugg uggaaucucu ucgcgccugg     2640

auugcugccg gaaagcccaa ggacagaccg ccucucagcc ccaaaggcga uguuauccgg     2700

aaggugcgca uucagacaaa ugacaaaguc uccguggaga ucaacggggg aaccguugau     2760

agaggcgaca uggcucgggu ggaugucuuu cgcaagaaaa auaagaaggc cgugugggag     2820

uacuauguug ugcccauuua cccucaccag aucgucgccc ugaacgaccc gcccgauaga     2880

gcugugaucg cguacgccga agacaaagau uggaaggaga uugacucuag cuaugaguuc     2940

cugugguccc ucuuugggcu gagcuacgug gagaucucua aagcuaaugg cgaaugcauu     3000

gacggauauu uccggggccu ucaucgcggg acaggagccg ccuccgucug uaagcacauc     3060

agccugggca aagaugcuac cguuucuggg auuggacuca agacgcuggc cuccuuuaag     3120

aaauucacaa ucgacagacu gggccggaag uuugagaucc cuagagaggu gcgcaccugg     3180

cggggcgaag cgugcacaug a                                               3201


<210>  3833
<211>  3201
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3833
augagccacc uggugcucgg ccuggaccuu gggcccaacu ccaucggaug ggcccugguc       60

aaugaugacc cugagcaucc gguugaaucu aagcucgugg aucugggcgu gagagucuuc      120

cccgaggggg uggacaacuu ugauaccagc aaagaacuuu cccggaauga ggaccgcaga      180

guugcucggg gaaugcgcag acagauucgg cgcagagccc ggcgcagaca acagcugaag      240

caggcgcuca ucgauucuaa acuguggccu agcgacccca acgaugaagu gguccuguac      300

aauacaaacc cuuaugagcu ccgggcucgc gcccugaagg agagacuuga accccacgag      360

gugggccggg uucugcucca ucugaaucag cgcagagggu uccuuuccaa ccggaaaaag      420

gacagaggag auaaggaagu gcaaggcaug cuggccgaga uuaaugagaa cgaacagcag      480

ugcaaagacg gcggguuuga uacgaucgga gcuuggcucg ccgacaagca ggcuaauauu      540

gaccacgcca accgcgagga aaaugaucau guccggaaca gacaccuggc gcgcucucaa      600

uacguggacg aguucgaagc caucuggagc gugcaggcua aacaccaucc uaagcugcuc      660

accgaggagc ugaaagucgg caaacuuggg aagcagacau auccgguuaa gcccaucccu      720

cgguggcacg aacauagaca gggacugagc ccccucgagu ccuuuggcau uuacgggcug      780

aucuucuuuc aacgcccuau guauuggccc aagucugugg ugggacuuug ugaacuggag      840

ccgaaagaac ggagaugcca gcgcuccgau cggagacagc aacgcuuccg gcuccugcag      900

gaggucaaua accugaagua cguggacccu gauacccacc ucgagcagaa acuggacgau      960

aagcagagac ggcuucugcu cgacaaacug agccacacaa aggaaaugac cuuugaucaa     1020

auucgcaagg cccuugacuu ccuggagucu guuaaauuua accucgaaag aggcgagcgg     1080

uccagacuga agggcgugcc caucgaugcu cuguucgccg cgaaagacgu ccucgggcag     1140

gaaugguaug accgcccuga gcagcagaag acagagauug uggccguucu gcuugauaau     1200

gaacgggacg augacagaau cauugcucgc gccguggcug aguggggaau gaccgccaaa     1260

gaagccgagg cuaugcuggg cgucgaucuc cccgcggggu acgccaaccu gagcuguaag     1320

gcuaucgaga agcugcuucc ccauauggaa agaggacucc uguacaugga cgaugacgag     1380

ucuaauuccg ccaugcacgc cgcuggcuau cugcggcgcg aucaacucag acggcgcauc     1440

uuugacgugc ugccugaucc ggccagaacg cccaacugcc cuauugggga cauccccaau     1500

ccugucguga aacggacccu uacagaaauu cgcaagguug ugaacgcgau cgucagagag     1560

uacggaaaac ccgaugcuau ucaucuggaa cucgcccgga gcgugcagau gggcaaggag     1620

aaacgcuccg aguauucuaa gaugaucaga gaccgggaag ccgagagaga cggcaucguu     1680

gaaaagcugc gcgagagcgg ggugcggguc accagagaua auauucugaa auaccagcuu     1740

ugggaacagc aaaaccagga guguaucuau uccggaaagc cgauuagcuu cgagaaacuc     1800

uucggcgaag ggggaggcgu ggagguggac cacauccugc cucgcucucg gucccuggau     1860

gacagccagg cuaauaagau uguuugccuc agaacagcca acgcugggaa aggcgaucag     1920

acccccuacg aauggcuggc cgacacgcaa ccuaagcagu augagcaggu cugucaacgc     1980

gcgggaaugc uuaugcgguc uggcagaaug cccuacgcca aguaccgccg guuuauccag     2040

agagaacugg agcucgauaa auucaucgcu cggcagcugg ccgacacagg guauauuacc     2100

aaggcuacaa uggaguaccu gcgcuaucuu uuugauaaag accaugaugu gcucggacug     2160

aagggccagc ugaccgccga acucagauac cacugggggc uggagaccau ccuugaggaa     2220

cugccugaca gccccgcgug gcacgaggcc gauuccggaa aacuccggcc gggcgaaaag     2280

aaucgcgcug accauagaca ccaugccauu gacgcugugg ucaucgcccu gacaaaccgg     2340

agacgccugg ugcaacuuuc ugauauuguu aaaagagggg gcgcccgggc ucacggagag     2400

auccucgaag acccuuggcc caauuuccgc gagaacgugg uccaggcgau uaagaagacg     2460

aaugugagcc accggguuga aagaaaagug gccggcaagc ugcaugagga gacccuguau     2520

gggcccacac gcaccccugg agaauggguc cuccggaaac cggugaucgc ucuguccgcc     2580

aacgagguug aaagaauccg cgaugccaca auuagacaga ucguggucag cgcucuugcc     2640

gacgcgggca uugauguggg gcggggaaag aaacccgacg cuaagaagau gaaagaggcc     2700

cugucuaagc ucaaaaugcc uuccggcgug cccauccgca aggucagacu gaccaaaccu     2760

gaacagacga uucaacccgu ucgggccggg cgcccugauc aggcuuuugu gaagcccggc     2820

agcacacacc aucugugcau cuucgaguuu accgagaacg gaaagaccaa aagagacgcc     2880

guguucguca caaugcuuga agcgaugaau cggcugaaga gacgcgagcc ggugauccag     2940

cggacccacc cugaaagacc cgauucucgc uuuguuaugu cccucagcgc ucgggagaug     3000

cugcucgcca acuggaaaga cggcgaaaga cugcuuacau ucaagacggc cuccucuacc     3060

caggggcaaa uuugguuugc ugagcauaca gaugcccgga aaagcgcuga cuacaagcag     3120

uucguggcca augcgaacac ccuggacgcc aagaaaguca caguggaucc ucucggacgc     3180

aucagauggg cuaaugacug a                                               3201


<210>  3834
<211>  3204
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3834
augggcacca gacuggggcu cgacaucgga acaaacagca uuggcuggug ccuguucaau       60

cuugaugggu ccggacggcc caucggcauu cgcgacaucg gggugagaau cuuuucugau      120

ggacgggacc cucgcagcgg cgccucccug gcugucgaga gacggguugc ccgcgcgaug      180

agacggcgca gagaucggua cauccgcaga cggucugcuc uccuggaagc ccuuaucaac      240

acgggccuga ugccggccga cgugcaggag agcaaggcuc ucaccgggug ugaucccuau      300

gugcugcgcg cccaagcucu ggacagagcc cucgaugcgc accuucuugg acggguccug      360

uuccaucuca aucagagacg cggcuuuaaa uccaaccgga aggccgaaag aaaagcuggg      420

gacgauaagg agggaggcaa aauugccgac ggggcuaagg cccuggaucu ugcgaugcac      480

gaagccggag cucgcacaua cggcgaguuc cuggccggcc gggacucuag acgcgugcgg      540

cucagagcug agggggacgg auaugauuuu uacccugaac gccggcaucu ggaggccgaa      600

uuugacgcca ucugggaugc ucaggcggcc uauaaucccg cucugcucac cgacguugug      660

agagaucgcc ugcggagaau uaucuucuuc cagcggccuc uuaaggcccc caaagucggc      720

gggugcacau uuuucaacga ggagccucgc cugccgaagg cccacccccu cuuucaagaa      780

agacggcugu acgaggaagu gaaucagcuu gagauuacca gagcuggagc cccuagccgc      840

aaacugacac ucgaccagcg ggauaagauc auuagaaaac ugaaguccgc gaagaccgcu      900

agcuucucug gccuggcccg ccuccugaaa cuugccgacg gggaauccuu uagcagaguu      960

acggagaacc ggaccggacu ggcuggcgau gacaucuuca caucuuucuc cgauaagcgc     1020

uguuuuggcg acagaugggc ccauuucgac cccggggugc aguggagcau cauugagacc     1080

gucacagaag aggaagaucc ugugcggcuc cgcgacuggc ugauggcuac ccacggagug     1140

acggucgagc aagccgaagc gaucggcaga guucggcuuc ccgaggggca cggacgccug     1200

ggcgagacag ccucuagacu cauucuggcu cagcugaaau ccgggagcaa ggauggccau     1260

ccgcucaccu auaaugaagc cguggaggcu gcccugggaa aaucccacuc ugacuuucgg     1320

acaggcgaaa uccuugagca gcugccuuac uacggggaaa uucucagccg cgagauuccc     1380

ccuggauccc uggaucccga cgaugacgau gagaucagau ggggcaagau uaccaacccc     1440

accgugcaua ucgggcugaa ucagcuucgg agacuccuga acgcgcugau uaaagcccac     1500

ggacgcccug acgaaaucgu cguggagcuc gcucgggaac ugaagcugag cgagaaggau     1560

aaagccgagc acaauagacg cauuggcgcu aacacacggg ccgccgaagc uagaucuaag     1620

aaacugcucg aguccgggca accggacacg ggcgcgaauc gcagccugcu gaaacuuugg     1680

gaagaucuca accccaacaa uccucuggac cggugcuguc ccuucugcgg aagacauauc     1740

ggccagaugg cccuguuuuc uggggagacc gaaaucgagc acauuauccc uuauagccgc     1800

ucccucgacg auucugcugc caacaagguu auugcccauc ggagcuguaa uagagagaag     1860

ggaaaccgga cacccuggga ggcuuggggc cacaccgaac gcugggccau cauugcggaa     1920

caggugucca gacugcauaa gagcaaacaa uggcgguucg cuccggacgc caucgagcgc     1980

uuuaaucagg aagggggauu cauugccaga cagcuuucug auacacagua ccugucccgg     2040

auggcuagaa ccuaucucag cucucuguac ccugacacgg ccggcgucua ugugaucccc     2100

gggcgcauga cagcgaugcu gcggagacuu uggggccuca acgagcugcu gccugaucac     2160

aauuacguug agaaccccca cuccggagcu ccuaagaauc gccucgacca ucggcaccau     2220

gccaucgaug ccgcuguggu cggcgugacc accagagggc ugcuuaacga aauuagccgc     2280

gccgcuggac gggccgagag acaagaccug gaucgccucu uuaucgaccu gcccaaaccg     2340

ugggaaacau ucagagagga ucugcacgaa ucccuugcgc gggucguggg cucucacaag     2400

gccgaccaug ggcgcaaagg aguuccugcu aagggcaagg augccaccgc ugccagacuc     2460

cacaaugaga cagccuaugg gcugacgggc cugaccagcg aguccggacu ccccauugug     2520

guccaucggg ugccccugcu uucucugaaa ccugacgacc ucagcaaccc gauccgcauu     2580

cccgaugcug cgcugcagag agcccugcgg gaagcuacag acggcaaguc cgggaaagau     2640

uuucaggagg cccuugccca guucagcaag ucugacccug uuuuuaaagg aaucagacac     2700

gugcgcgucc gggaaacccu gaaggugauc cccauuagag augcuuccgg ccaugccuuc     2760

aaggcguaca aaggggacgc uaaugcccgc uacgaugugu ggcggaugcc ugacggaaag     2820

uggguuagcu cuuggagaga ucgggacggc aaagcccacu ccggcaucau uacaauguuc     2880

gcugcccacc aagcggggca gcccguccag cgcccucauc ccgcugccaa gagagugcuc     2940

agccugcggc agaacgaucu cguggccguc gagccgcaag gacagccucg caaaaucaug     3000

agagugguua aguuuucuag cggcgggcag auuaccuucg cugagcacca ggaagccgga     3060

ccccugaaag cucgggacgc cacguccccu gacaucgauc ccuuuaagua uuucuacagc     3120

ucugcgggcg ggcuucaaaa ggcccgcgcu agacagaucc ggauugacga gcugggacgc     3180

aucuuugauc cgggcccuag auga                                            3204


<210>  3835
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3835
augaaguacg ugcugggccu cgacaucggg auugccagcu gcggaugggc ugucaucaac       60

caggagaaac acagaauuga agaucugggc guucggaucu ucgacaaggc cgagaauccc      120

aaagauggga agucccuugc gaccccucgc agagacgcuc ggucuacacg cagaacgcug      180

cggcgcaaaa agcauagaau gcaacggauu aagauccucc uggugaaaca cggacuucug      240

agcaagaccg aaaucgauca ucucuaugag uccgccacag aaauugacgu gugguaccug      300

cgccugaacg cccucgagag acggcugaau ccgaaagagu uugcucgcgu ccuuauccac      360

cugucuaaga gacggggcuu caaaagcaac cgcaaggaaa ccacacucuc cgagaauggg      420

cagauucugg aaaacaucuc ugagaaucuu cagauuaugg agcagaagaa cuauagaacc      480

gugggagaaa ugauccugaa agauaagcgg uuugagaauc auaaaagaaa caaggacggc      540

acauacauug gcaccguuac gcgccaacag cuccaggaag agauccagau gaucuucaau      600

gcccaacggc uguauaaaaa caaucacgcu accgaaaagu uugagagcuc cuaccuggag      660

auuugggcca gccagagacc cuaugcgucu aaagaucaga ucgaaaagau gauuggguac      720

uguacacucg agccuaagga aaaacgcgug cccaaggccu ccuggagcuu ccaguacuuu      780

gucgcucugc aaaccaucaa caaucuucgg cugauuucua aagacagaau cgaggaacuc      840

uccuucgagg agaagaaacg caucaugaac cuggcccuug aaaagagcau ugugaaguau      900

aucgauauuc ggaaacugcu cucuguuccu aaggaguuuc aguucaacuc ccugagcuac      960

uccgcugaca ucguggauac aaccgucgaa aauaaaaagu gcauugagcu gaaagaauau     1020

cacucuauca acaaggugua caagcagauc uauggacaaa augcccucaa ccugcuuccc     1080

aaagacuacg acacgauugc guauggccug acaaucuuua aggaugacaa agauauucag     1140

gaguaccucc agaauaagua cgugaacgcc aaagggaagc cuaucagcaa ucuggcuaac     1200

aagaccuaug acgauacauu cauucagguc cuucugaccc ucaauuuuuc caaaauggga     1260

caucugagcu ucaaggcccu gaaaaacauc auuccguucc ucgaggaagg ccugucuuac     1320

gacaaggcuu gugagaaagc cggguauaau uuuaagggaa ccuccuacac aaagaagacg     1380

aaguaucuuc ccguuauccc ucaaaacacc aaucccgugg ugcacagagc ccugagccag     1440

acacgcaaag ucaucaacgc uauuaucaag aaauacggcu cuccuaaugc gauucauauc     1500

gaaaccgccc gggagcucag caagacauuc caggagagaa aagaaauuga uuccauguau     1560

caggacaacu cuaagaagaa cgagcacgcu aucugcaaac ugaaggaacu gggcaauauc     1620

aaccccagcg ggauuaauau cgugaaauuu aaacuuugga acgagcaaga uggacgcugu     1680

auguacaccg gcaaauacau ugaaccgcac agacucuucg aggaggggua uacggaaguu     1740

gaccauaucc ugccuuacuc ccggagccug gaugacucuu auaauaacaa ggugcucaca     1800

cugggaaccg agaaucagcg gaagggcaac aaaacccccu acgaauauau gcgcaauaac     1860

uccauuuggg acgaguuuga aacaagaguc cagagcaaua agaaaaucaa caagaaaaag     1920

cagcaaaaac uucugcucca gaauuucucu cacgcccggg agcaggaguu uaucgaaaga     1980

aaccugaaug auacccgcua cgccacaauu uaucugucca gccuuaucca gcaacaucuc     2040

auuuucuccg agucuagccg ggaaaagaag gugcacacgg uuuccgggau cauuaccucu     2100

caucugagaa gccgcugggg auuuaacaaa gacagaaagg agggccacau ccaccaugcu     2160

cuggaugccg ugaucgucgc ggugacaucc gaccacauga uucagcgggu caccaaauac     2220

uacaagcuca aagaacugaa ccgcaauaga caggcuaagc ggaugcaauu cccugagccc     2280

ugggagggcu uucgccuuga acuggaggcc agaaucagcc ccaacacaca gcaguaucuc     2340

aaggggauuc gguucaaaaa uuacgccgau gugaaccugu cugaaaucaa gccuauuuuu     2400

guuucccgca ugccgaaaag aagcaucacc ggagagcugc aucaggagac gauucggaag     2460

uuccuuggcu auaaugaaaa agggaaggug cucaccgcua caaagaccaa acuggaggac     2520

auccccuucg augccaacgg agacuuuccu auguucggca aggaaacaga ucuguacacc     2580

uauaaugcua ucaaagagcg cuaccucucu cacaagaaag acaaguccaa gagcuuucaa     2640

gaaccccugu auaaaccuac gaagucuggg ggcauuggac cccuuaucaa aagcauuaag     2700

aucauggaua caagaaccau ugucaacccg gugaaucagg gcaaaggggu uguguacaac     2760

uccaagaucg cccggacaga cgucuucaag aaaggagaga aguacuaucu gaucccuauu     2820

uacaccaucg aucucaugaa aaauacccug ccccagaagg cgauuacagc cggcaaaggg     2880

uaugaggacu ggacgaccau cgacccuagc uuuacauucc uguuuucucu uuuccccaac     2940

gaucucauuc agaucgugcc uuccaagaau aagaccauca aagcuagaac aaccgugagc     3000

aagaaagaag uucugcugcc cucccucacg ggauacuuua agggcgucca cucugggaca     3060

gccggcauua ccguggagac ccaugacgga agcaucauug cuaacguggg cuccaaacaa     3120

cugcuuucuu ucgaaaagua ucaggucgau gugcuggggc acuacacaaa aaucaagaag     3180

gagaaacgca uuggaauggc caucuga                                         3207


<210>  3836
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3836
augaacaagg ccguggcuua cagacuggcc cucgaccugg gcagcaccuc ccuugggugg       60

gcgaucuucc ggcugaauga ggcucgcgaa cccacagcca uuaucagagc cggaguccgg      120

auuuucucug auggccgcaa cgcuaauagc gagccucucg ccguucacag acggguggcu      180

cgcgccauga gacggcgcag agaccggcug cuuaaacgca agaaaagaau gcaugaucag      240

cuggugcaac acggguucuu uccggcggaa gucgccgagc ggaaggaacu cgagcgccug      300

aaccccuauc agcugagagc uaaaggacuc caugaggccc ugacgccugg cgaauuugcu      360

cgggcccuuu uucacaucaa ucagagacgc ggguucaagu ccaaccggaa gaccgacaga      420

aaagauaaug acucuggagc gcugaagcag gccauuagcg agcuccgcaa acggauccaa      480

gauuccgacu gcgcuacagc cggcgaaugg uuuuggaagg agagaaugca gcagaaaccc      540

gagggcgugc gcgggcagug gguucgggcu agauaccgca agaccccuuc uacaaccgau      600

gaaggaaaga aacggaucgg cuaugaccug uacguggaua gagccauggu cgagcaagag      660

uucgacgccc uuugggcugc gcaggccgcu cugcagcccg ggcucuuuac agaagccgcc      720

cgcgcugaac ugaaggauac ccugcucuau cagcgggacc ugagaccugu gaaaccggga      780

cgguguacgc uucugcccgc cgaggagcgc gcgccucucg cucugcccag cacccaaaga      840

uuccggauuc uucaggaagu uaacaaucug agacuccugg acgaggcccu gcgcgaagug      900

ccucucaacc uggcccagcg ggaugcuguc guguccgccc uugagggcaa gaaagaacug      960

agcuuugcug ccaucagaaa gcuccugaca cuuucuggga aauucaaucu ggaggacgag     1020

aagcgcgcgg aacucaaggg aaacgccacc uccgugauuc uggcuagaaa agaucuguuu     1080

ggcgacgccu gggcuggcuu cgaugccgcg guccaagacg agaucguuug gcggcucgug     1140

agccaggaau cugagggggc ccugauugcu uggcuucagc agcauacagg aguggauggc     1200

gucugcgccg aagcuaucgu gaauacccgc cugcccgacg gguacggaag acucucccgg     1260

aaggcccugg agcgcauugu uccggcccug caaagagagg uguguacgua ugauaaagcu     1320

guccaggcgg ccggcuucgc ucaccacagc gaccuugggu uugauuucga cuacgccgaa     1380

gacgaggugc agcagguugg agaacauaca aucgccucua ccggcgaggu gcaagcucag     1440

uacgccuuua agcagcuccc uuauuacggc aaagcgcugc agcggcacgu cgcuuucggg     1500

uccggagaug ccaaggacca ugaggaaaag ugcuauggca aaaucgccaa ccccacagug     1560

cacauugggc ugaaucaagu ccgcaccgug guuaacgcuc ucaucagacg guacggacac     1620

ccuaccgagg uggucgugga acuggcccgc gaucuuaagc agagcagaga gcagaaacag     1680

caaacacagc gggaacaggc ggacaaccaa aagagaaaug aggauauucg caaacggauc     1740

gcucccauuc uggagacguc ccccgaaaga guucgcgacc gggauaucaa gaaguggauc     1800

cucugggagg aacugaacaa aaaggacauu gccgauagac auuguccuua uucuggcgag     1860

cgcaucagcg ccaccaugcu gcuuuccgaa gcuguggaga uugagcacau ccucccguuu     1920

agccggacac uggacgauuc ucugaauaac agaaccgucg ccaugcgccg ggcuaauaga     1980

auuaaagggg accggacacc cugggaagcc cgcgcggauu ucgaggccca gggcuggaga     2040

uacgaagcua uccuccagcg ggccgaggga augccucccc gcaagagaua ucgguuugcu     2100

gaagacggcu accagagaug gcuggggaaa gaccgcaacu uccuugcccg ggcccugaau     2160

gauaccuccu accucagcag acuggcugcg aacuaucugc gccuugugug cccucaagga     2220

gugcggguua uccccggcca gaugacggac aagcucagag ggaaguuugg acucaauucu     2280

guccugggcc uggaugggaa aaagaaccgc aaugaccauc ggcaccaugc cguggaugcu     2340

ugugugauug gcgucacaga ccagggacug augcagagau ucgccaacgc cagcaaacaa     2400

gcucgcgaga auggccuuac cagacuggug caggauaugc uccugccgug guggccuucc     2460

uacuaugacc acguugagcg ggccgugcgc cacaucagag ucucucaucg gcccgaucac     2520

ggguuugaag gagcgaugau gaagggcacc gcucauggga uucgcgagga cggaaucaga     2580

gaagauggcc ggauuaaaca gagaccuaag gccaaaggga gcgccgacca caagacaauc     2640

acccugauuc ccaucgauga gccucgccag cuugcucggc acggcgugga cgccgaggga     2700

aagccccucc cguacaaagg cuaugcgucc gggagcaacu acugcaucga aauuacaaag     2760

aauggaaaag gcaaguggga ggggcaaguu aucucuacgu ucgacgcuua uagaauuaaa     2820

gccgccgcug augccgcugc ccgcgcggga caggugaucu ccaccgucga agccgacagc     2880

auugugcgga agucuggcug ggagagacug cgcggggcuc agucccaaaa cggccagccu     2940

cugguuaugc ggcucgugau cggagauagc gucagaaugg aaguggacgg ccgggaugag     3000

gugaugcgcg ucguuaagau guccgggaaa gagauggugu uugcccccgu gagagaagcu     3060

aacgucgaca agcggaauaa caugcccgau gagcaggacc cuuucacaua caccuacaaa     3120

cgcgccgauc agcugagaaa ggccaaagcu cggcaaguga caaucucucc gauuggagaa     3180

cuucgcgacc ccggcuucaa gggguga                                         3207


<210>  3837
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3837
augcaguacg ugcugggccu cgacaucggg auugccagcu gcggaugggc ugucaucaac       60

caagagaagg aaagaauuga ggaucugggc guucggaucu ucgacaaagc cgaaaauccc      120

aaggauggga aaucccuugc ggcuccucgc agagacgccc ggucuacccg cagaacacug      180

cggcgcaaga aacacagaau gcuccggauu aagauccugc uugugaagca gggacugcuc      240

agcaaaaacg agcugaauca ucuguaugaa uccacgaccg agguggaugu cuggaaccuc      300

cgccuggacg cccuugagag aaagcugaau ccgaaagaau uuacacgggu gcucauccac      360

cuggcuaagc gcagaggcuu caaaucuaac cggaaggaga ccacacuuag cgaaaauggg      420

cagauucugg aguccaucuc ugagaaccag caaauuaugg aacagcagaa uuaccgcacc      480

guuggagaga ugauccucaa ggauaaaaag uuugaaaacc auaaaagaaa uaaggacggc      540

acauauauug gcaccgugac gcggcagcaa cugcaggagg aaauccagau gauuuucacc      600

gcccagagac uguacaaaaa cgauuaugcu acagaggagu uugaaaucag cuaccucgag      660

aucugggccu cccaacgccc cuaugcgagc aaggaccaga uugagaaaau gaucggguac      720

uguacccugg aaccuaagga aaagcggguc cccaaagccu cuugguccuu ccaguacuuu      780

guggcccuuc aaacaauuaa uaaccugaga cucaucagca aggaucgcau ugaggagcug      840

ucuuucaaag aaaagaaaca gaucaugaau cuugcucugg agaaguccau cguuaaguau      900

auugacaucc ggaaacuccu gagcauuccu aacgaauuuc aguucaacuc ucuguccuac      960

agcgcugaua ccguggacac gacacucgag aauaagagau gcaucgaacu gaaagaguau     1020

uacuccauua acaaggucua uaaacagauc uacgagaaga augcccuuaa ccugcucccc     1080

aucgacuaug auaccauugc guacggacug acaaucuuua aggacgauaa agacauucuu     1140

gaauaccugc aaaauaagua ugugaacgcc aaaggcaagc cuaucaauaa ccucgcuaaa     1200

aagaccuacg auuauaccuu cauucaggcc cugcugacac ucaauuuuac gaagaugggg     1260

caccugucuu ucaaagcucu uaagaacauc auuccguucc uggaggaagg acucagcuac     1320

gacaaagccu gugagaaggc cggcuauaau uuuaaaggga ccuccuacac agagaagacc     1380

aaauaucugc ccgugauccc ucagaacaca aaucccgucg uucaucgcgc ucugagccag     1440

acccggaagg ugaucaacgc gauuaucaag aaauacggau cuccuaaugc cauucacauc     1500

gaaacggcua gagagcuuuc caagacauuc caagaacgca aagagauuga uagcauguac     1560

caggacaacu cuaagaaaaa cgaacacgcc auccauaagc ucaaggagcu gggcaauauc     1620

aaccccagcg gcauuaauau cgugaaauuu aagcugugga acgagcagga ugggcggugc     1680

auguauuccg gaaaauacau ugaaccgcac agacucuucg aggaaggcua uaccgagguc     1740

gaccauaucc ugccuuacuc ucgcagccuu gaugacuccu auaauaacaa gacccugaca     1800

cucgggaccg aaaaucagcg gaaaggaaac aagacacccu acgaguauau gggcaauacg     1860

agcauuugga acgacuuuga gaccagagug cacucuaaua agaaaaucaa caagaaaaag     1920

caacagaaac ugcugcuuca gcacuucucc uacgcccggg aacaggaguu uaucgaacgc     1980

aaucucaacg auacaagaua cgcuaccauu uaucugagcu cucugaucca acagcaucuc     2040

auuuucuccg agagcucccg gaagaagaaa guucacacag ugucugggau cauuaccagc     2100

caucugagau cccgcugggg auuuaauaag gaccggaaag aaggccacau ccaucacgcc     2160

cuugaugcgg ucaucguggc uguuacgucu gaccacauga uucagagagu gaccaaguac     2220

uauaaacuga aggagcucaa ccgcaaccug caagccaaaa gaaugccuuu ccccgagccc     2280

ugggaaggcu uucggcugga gcuugaagcc cgcaucagcc cuaauacaca gcaguaccuc     2340

aagggguuca gauuuaagaa cuaugcugau gucaaucugu ccgagauuaa accgaucuuc     2400

gugagccgga ugcccaagcg cucuauuacc ggagagcugc aucaggaaac aaucagaaaa     2460

uucauuggcu acaacgagaa ggggaaaguc cucaccgcca ucaagacgaa gcuggaagac     2520

aucccuuuug augcuaaugg agacuucccc auguauggca aagagacaga ucuuuacacc     2580

uacaacgcca uuaaggaacg guuucugucc cacaaaaagg acaaacgcaa gagcuuccaa     2640

gagccucucu auaagcccac aaaaucuggg ggcaucggac cgcugauuaa guccaucaaa     2700

auuaucgaua ccagaaccau cgugaauccu guuaaccagg gcaagggggu ggucuacaau     2760

agcaaaauug cgcggacaga cguguuuaag aaggaugaga aauauuaccu gauccccauu     2820

uauacgaucg accuuaugaa gaacauucuc ccucagaaag ccaucaccgc uggaaagggc     2880

uacgaagacu ggacaaccau cgaucccucu uucacauuuc uguucagccu guuuccuaau     2940

gaccucauuc agaucguucc cuccaaaaac aagaccauua aggcccgcac gacagugagc     3000

aaaaaggagg uccugcuucc gucucugacc ggguauuuca aaggagugca uuccggcacc     3060

gcugggauca caguggaaac ccacgauggc agcguuauug ccaaugucgg auccaagcaa     3120

cuccugcuga ucgagaaaua ccagguggac auucuuggcc acuacacaaa gaucaaagaa     3180

gagaagagaa ucgggauggc gauuuga                                         3207


<210>  3838
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3838
augcccaaga gcgccaaacc ucagcaggcu uacacccugg gccucgacau cgggauugcc       60

uccgugggag cggcucugcu uggccaacag cacaucauug ggcugcaugu cagaacauuc      120

gaucgggccg agacggccaa ggaaggagag ccgcucaaca aaauccgcag agacgcucgg      180

cugacccgca gacggcuuca gcgcagagcc caccggcugc uccgccugag acggcuguuu      240

aagcgccaua aucucauucc cgauacagac accucuacau ucaucagcgg ccaauccccu      300

ugggaacuga gagcucaggg gcuugaucgg cagcugaccu cugccgagug gacaaccguu      360

cucuaucaca ucgugaaaca ucgcggauuu cacagcaaca gaaaguccga agugaagacg      420

gacgagaaag cgggccagau gcugucuggc gucaaucaaa accaggcccu ucugaagcag      480

agcgggcucc ggaccauggg agagcuggcu gccagacacg aggcuuuccg cgaagccaaa      540

cggaauaaga aaggcgcgua caaccauaca uuuuccagac acgaucugga agacgagcuc      600

caucugcuuu ucagccgcca gcgggagcug gggaaucccu augccacccc ugaacucgag      660

gaugcugugc acacacugcu uaugcaaaga cgccccgccc ugucuggaac caaccuccug      720

aagaugguug gccacugcac gcuggaaccu gacgaguuuc gggcuccgaa ggccuccuac      780

acagccgaaa gauucauuug gcucaccaaa cugaauaacc uucgcgugag cgggucugga      840

gagacacggc cccugaauga guccgaaaga caggcucucc ugagcaagcc uuuuucucag      900

uccaaacuua ccuauaagca aguccgcaaa auccuggagc uccccgauaa cauucgguuc      960

gugggccugc auuaccaccc ugcgcaggac aauggcaaga gccccgaaaa cagaguguuu     1020

uucgaggcca aggcuuuuca ugcccugcgg aauguuuaua aaaaggccgg gcucaaccug     1080

gaauggcagc gcgauuccca gaacccggag aaacuugaca auaucaccua cgcucugaca     1140

gucuucaagg aggacaaaga agccaagcaa uggcucauug agaaaggagu ggaagcgucu     1200

gugaucgagg cuguccugaa cgagagcuuc uccgaauuug uuagacugag cauuaaggcc     1260

cuucggaaga ucaucccuca cauggagauu ggccauagau acgaugaagc cgugaaacuc     1320

gcuggguauu cucaccacuc ccaucugccc cagccuagca agacgcccua caucccccgc     1380

uucucuaaac agagcauugc caauccugug gucucccggg cucugaacca ggccagaaag     1440

cucgugaaug cgaucguucg cgaguaugga ccgcccgacg ccgugcacau ugaacuggcu     1500

agagaucuua acaaaccuuu ugacgagcgg cgcaagaucg agaaggauca agaaaaauac     1560

cagaaggaca aagccagaga uaucgagacc uuccaggaaa auuuuggcuu cacacccaag     1620

gggcugcagc ucaccaaaug gcggcuguau cgcgagcaau cuggaaagug ugcuuacagc     1680

cugaagccuc uugacauuaa cagacucuuu gaagauggcu augucgagau cgaccaugcc     1740

cugcccuacu cccggagcuu caaugauggc augaacaaua aagugcugac acucaccacg     1800

gagaaccgca auaaggggaa ccagacaccg uacgaauauc uggacggagc gucuaauucc     1860

gcccagugga gaaacuuugu ugcuugggug aacagcaaua aaucuuaccg ggccuccaag     1920

cagcgcaacc uucugagaaa agacuucgcu gcccaagccg cuaccgaguu ucgggaaaga     1980

caccucaccg auacacgcua uauugcgcgg gaguucaaga gaauggucga aacccaccug     2040

aaacugaaua agccuaacca gcgcugcgug gucgugagcg gccagcuuac agccuaccuc     2100

cgggcuagau gggggcugau caagguucgc gagaauggag accugcauca cgcccucgau     2160

gccgcugugg ucgccgcgug uucccaugcu auggugaaac ggcuggccga cuauucuaga     2220

cgcggcgagc uucaauacgc ccgggggcac cauuuugauc ccgaaacggg cgagauuauc     2280

gacaucaacg cucugagaaa gcucgaugac cacuucccuc agcccuggac cgauuuccgg     2340

cgcgaacugc uggccuggcu uagcccugac cccgcgcagc agcucaaaaa ucuggcuuau     2400

uacacagaug agagacugaa gcaaguuaaa ccgauucggg ugucccgcgc cccuaccaga     2460

cggggacucg gcgccgcuca ccaggaaaca aucagaucug ccaaguaccu gggggagaac     2520

cgcagcaccg ucaagacgcc ccuugagaaa cugaagcuca aagacauucc caauaucgug     2580

ggauaugaag auccucggaa ccaucagcug auucaggcua ucucccaaag acugaccgag     2640

cacggcgacg acgggaagaa agccuuuaag gaaccgcuuu acaagcccgg aaauaacaaa     2700

agcacagcgc cugugguucg cucugucaag cuguucaccg ugcagaaauc cggcauuccc     2760

gugagagggg gcaucgccaa uaacggaaau augguccggg uggauaucuu uacaaagaaa     2820

gagcgcuauu ucgcuguucc uguguacguc agcgacgccg cucggaacga gcuccccaac     2880

aaggccgugg ccagaggcaa ggaugggugg auugacaucg augacucuuu uuccuuccug     2940

uuuagccucu auccuaauga uuggguuucu guggucguga aaccccauga agcuccgugc     3000

gagggauacu augcgggccu ggacauuagc accggggcca uccacauuug ggcucaugau     3060

cgcaacagau ccguuggaaa gaauggccuu augcggagca ucgggaucaa aacggcccac     3120

ucuguggaaa aguaccacgu cgaccugcuc ggcaaccugu accgcguggu gcaugagaca     3180

agaaaaccuc ugcgggccgg aaaguga                                         3207


<210>  3839
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3839
augcaguacg ugcugggccu cgacaucggg auugccagcu gcggaugggc ugucaucaac       60

caagagaagg aaagaauuga ggaucugggc guucggaucu ucgacaaagc cgaaaauccc      120

aaggauggga aauccauggc gaccccucgc agagacgcuc ggucuacacg cagaacgcuu      180

cggcgcaaga aacacagaau gcagcggauu aagaucaucc uggugaagca gggacuccug      240

agcaaaaccg agcuugauca ucuguaugaa uccgccacag agguggacgu cuggaaccuc      300

cgccuggaug cccuggagag aaagcucaau ccgaaagaau uuacccgggu gcugauucac      360

cuugcuaagc gcagaggcuu caaaucuaac cggaaggaga caacccugag cgaaaauggg      420

cagauccucg aguccauuuc ugagaaccaa cagaucaugg gacagaagaa uuaccgcaca      480

guuggcgaaa ugauucugca ggacaaaaag uuugagagcc auaaaagaaa caaggauggc      540

accuauaucg ggacggugac ccggcaacag cuucaggacg aaauucagau gaucuucaau      600

gcccaaagac uguacaaaaa caauuaugcu acagccgagu uugaaaacuc cuaccucgag      660

aucugggcga gccagcgccc cuaugccucu aaggaucaga uugagaaaau gaucggauac      720

uguacccugg aacaaaagga gaagcgggcu ccuaaagccu ccuggagcuu ccaguacuuu      780

gucgcucugc agacaauuaa uaaccucaga cugaucucua aggaccgcau ugaagaacuu      840

uccuucgaag agaaaaagca gaucaugaau cuggcccucg agaaaagcau cgugaaguau      900

auugauaucc ggaagcugcu uucuguuccc aacgaauuuc aauucaacuc ccugagcuac      960

uccgcggaca ccguggacau uacggucgag ucuagaaaau gcaucgaacu caaggaguau     1020

cacagcauua auaaagugua caagcagauc uaugagaaaa acgcccugaa ucugcucccu     1080

aaggauuacg acacaaucgc uuauggccug accauuuuua aggaugacaa agauauccuu     1140

gaguaccugc agaacaagua cgugaaugcc aaagggaagc ccauuuccaa cuucgcuaau     1200

aaaacauaug acgauaccuu uauccaggcc cuccugaacc uuaauuucac caagauggga     1260

caucugagcu ucaaggcccu caaaaacauu aucccuuuuc uggaggaagg ccugucuuac     1320

gacaaggcuu gugagaaagc gggguauaau uucaagggaa cauccuacac ggagaaaacc     1380

aaguaucucc cggucauucc ccaaaacaca aauccuguug ugcaccgcgc ccugagccag     1440

acccggaaag ugaucaacgc uaucauuaag aaguacggcu cucccaaugc cauccacauu     1500

gaaacagcca gagagcuuag caaaaccuuu caggaacgca aggagaucga uuccauguau     1560

caggacaacu cuaaaaagaa cgaacaugcu auuugcaaac ugaaggagcu cggcaauauc     1620

aacccuagcg ggaucaauau ugucaaguuc aaacugugga acgaacaaga uggacggugu     1680

auguacuccg gcaaguacau cgagccccac agacuguuug aggaagggua uacggaggug     1740

gaccauauuc uuccguacag ccgcucucuc gacgauuccu auaauaacaa aacacugacc     1800

cugggaaccg aaaaucagcg gaagggcaac aaaacaccuu acgaguauau ggggaauacc     1860

agcaucugga acgacuucga aacaagaguu cagucuaaua agaagauuac gaaaaagaaa     1920

cagcaaaagc uccugcuuca gcacuuuucc uacgcccggg agcaggaguu caucgaacgc     1980

aaccugaaug auaccagaua ugcgacaauc uaccucagcu cccugauuca acagcaccug     2040

aucuuuucug agagcucccg gaaaaagaag gugcauaccg ucucuggaau uaucacaagc     2100

caccuuagau cccgcugggg cuucaacaaa gaccggaagg aaggccauau ucaccaugcu     2160

cucgaugccg ugaucguugc cgugaccagc gaccacauga uccagagagu cacgaaauac     2220

uauaagcuga aagagcugaa ucgcaaccuc caggcuaaga gaaugcccuu uccugaaccc     2280

ugggaggggu uccggcugga gcuugaagcc cgcauuucuc ccaacaccca acaguaccug     2340

aaaggauuua gauucaagaa uuaugcugau gugaaccucu ccgagaucaa gccuauuuuc     2400

gucagccgga ugccgaaacg cucuaucaca ggcgaacugc accaggagac cauuagaaag     2460

uuuaucgggu acaaugagaa aggaaaggug cugacagcca uuaaaaccaa acuugaagac     2520

auccccuucg augcgaacgg cgacuuuccu auguauggga aggagacgga ucucuacaca     2580

uauaaugcca ucaaagaacg guuccugucc cauaagaaag acaagcgcaa gagcuuucag     2640

gagccccugu acaagccuac caagucuggc ggaauuggcc cccucaucaa aagcauuaag     2700

aucauugaua caagaaccau cguuaacccg gugaaucaag ggaaaggagu cguguacaac     2760

uccaagaucg cucggaccga cguuuucaaa aaggacgaaa aguauuaccu gauuccuauc     2820

uauacaauug aucuuaugaa aaauauccug ccccagaagg ccauuacggc uggcaaaggg     2880

uacgaggacu ggaccacaau cgauccuagc uuuaccuucc ucuuuucucu guuccccaac     2940

gaccugaucc agauugugcc uuccaagaau aaaacaauca aggcccgcac cacggucagc     3000

aagaaagagg ugcuucuccc cucccugaca ggauauuuua agggcgugca cucugggacc     3060

gcgggcauua ccguugaaac acaugaugga agcgucaucg ccaacguggg cuccaaacag     3120

cugcuccugu ucgagaagua ccaaguggac guccuugggc acuauaccaa aauuaaggaa     3180

gagaaaagaa ucggaauggc uauuuga                                         3207


<210>  3840
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3840
augaagaaac agccccaaag auacauccug ggccucgacc uggggauugc cagcguggga       60

ugggcucuua uccuggucga uggcgccgag aacccuguug ggcuccugga cugcggagug      120

cggaccuucg aacgcgcgga ggugccgaag acaggcgauu cccuugcucu ggccagacgg      180

caggcccgcu cugucagacg gcucauucgc agacgggcuc accgccugcu gagacuccgg      240

cgccugcuua aaagagaagg gauccugagc gccgcugacu uuaauuccga uggacucccc      300

aaccugccua uugacgccug gcagcuucgg guggcgggcc uggagcgcaa gcucacgaau      360

caggaauggg ccgcuguucu gcugcaucuc gugaaacaca gaggcuaucu gucucaaaga      420

aagagcgaga cccagacaga gaacaaggaa cuugggagac ugcucgccgg agucgcugaa      480

aauuccaaac ugcuuucuga gagcuccgag caguaccgca cccccgccga gcuggcggug      540

aagcucuuug ccgaacagag cggccggcug agaaacaaag ggggagauua uacacauacc      600

uuuaaucgcc uggaccucca aaaggagcug caccuucugu ucgaacggca gugugcucuc      660

ggcaacccuu uugccucugc ugagcugcag uccaaaguug augaacuucu gaugacacag      720

agaagcgccc uccaagggga ggccgugcug aagaugcugg gaaagugcac cuucgagccc      780

ucugaauaca aagcugcgaa gaauacguau uccgccgagc gcuuuaucug gcucaccaaa      840

cugaacaauc uucggauccu guacaacggc gaagacagag cucucgauga gggcgaacgc      900

aagcagcugc uggacgagcc uuauaaaaag gccaaacuua cauacaagca gguccggagc      960

auucucaauc ugccggaguc ugccguguuc aaggggcuga gauacggaaa agauuccgac     1020

ggccucaagg cugaaaacgg ggcccugaug gagaugaaag cguaucauaa gauccgcacc     1080

gugcuugaaa aagcuggacu gaagggcgag ugggaaaauc ggauuagaaa ccccgagauc     1140

cucgaccaaa uugggacagc cuuuagcguc uacaagaccg augaggacau caaagccgaa     1200

cuggcugccc ugcggcuucc ugagaacguu cucgaugcuc ugcuggccgg acucaauuuc     1260

gacaaguuua uucagcuguc ccuuaaagcg cugucuaaca uccucccccu gauggaacgc     1320

ggcaucagau augaugaggc cuguaaggag guguacggcg accaccgggc ucuggccgaa     1380

agcaaaaauc agaugcuucu cccucagauu cccgcuaacg agaucagaaa uccggugguc     1440

cugcgcacgc ugacacaagc ccggaaggug auuaacgcga ucauuagacg cuaugggucc     1500

ccucagagag uucacaucga aaccgcccgg gagcucggaa agagcuucaa agaucgcaag     1560

gaaaucgaga aaagacagga ggaaaaucgg aaggagcgcg aaagagcugc cgagcauuuu     1620

aaagaacugu ucccccgguu cacaggcgag ccuaagucug gggacauucu uaagcugcgc     1680

cucuaccagc aacagcaggg aaaaugccug uauuccggca uccccauuga ucuggggaga     1740

cuuaacgaga agggcuacgu ggaaaucgac cacgcucucc ccuuuagccg gaccugggau     1800

gacucuuuca auaacaaagu ccuggugcug ggaagcgaga aucagaagaa acgcaaccaa     1860

accccuuacg aauaucucga cggcgccaga gauucccagc gguggagaga guuucaggcc     1920

cgcguucagg ggugucgguu cccguacgcu aagaaacaac ggauucagac agcgaagcug     1980

gacucuaaga cggaacaggg auuucuugag cgcaaucuga acgauacccg guauaucgcc     2040

agauuccucu gcggcuuuau cgagaaaagc cugcaucugc aaggggcugg aaagcgccgg     2100

guguucgccu ccaauggcca gauuacagcc cuucugagag ggcucugggg ccugaaaaag     2160

guccgcgaag acaacgaucg gcaccacgcu cucgacgcca ucguggucgc guguagcacc     2220

gugucuaugc agcagaaaau uacaaaggcu augcaaagac gggagacccu ggaaacgauc     2280

aacacagaga ccggagaggu uaagacccgc auuccccagc cuugggauuu cuucagacag     2340

gaagugauga uccgggucuu uuccgacgcu ccccgcgagg aacuugccga gaaacugaau     2400

agcagaccug aagcucucca ugauuucgug ucuccccugu uuguuucccg ggccccgaac     2460

agaaagauga caggccaggg gcaccuggag accgugaaaa gcgcgaagcg ccuuuccgag     2520

aauauuucug ucgugaaaaa gccucugaca gcucucaagc ugaaagacau cgaaaacauc     2580

gugggauacc ccgaucggga gccugcccuc uauagagccc ugcaagaacg ccuucaggcu     2640

uacaaggacg aucccgccaa agccuucgcc gagccuuucu auaagcccgc gaaagacggc     2700

gggcagggaa gccugguuaa ggccguccgg auuaucgaaa auacgaagca uggccucaaa     2760

gugagaaacg ggauuaccga cacaaauggc gauauggugc gcgcugacgu cuaccagaag     2820

gccgauaaaa agggaaaacg gcaauacuuu aucgugccgg uuuaugcuug gcaaguggag     2880

aagaacgagc ugccuaccgu cgacugcaaa ggcuacgaaa uugaugacac auaugaguuc     2940

uguuuuuccc uguacaagua ugauaucauc gaaauucaga aucccgacaa caugucuaag     3000

gagcuuagag uguacuauau caauugcgau agcuccgacg ggcgcuucug gcuggcccac     3060

caugauaaaa gcgccaagga acagcaguuu agaauuucua cccagaacca gcccaucauu     3120

aaaaaguacc aaguugaccc ucucggaaaa gagauccggc uguguaaggc ugagaagcgc     3180

acggaacuca auggcaaaaa gaaaaaguga                                      3210


<210>  3841
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3841
augaacuacg acaucggccu gagaauuggg aucaccagcu gcggaugguc cauuaucaau       60

aaggaucucg agcggauuga agaccugggc gugcgcgucu ucgagaaagc cgaaaacccc      120

gaugggacag cuucugccgc gccuagacgg gaggcucgca agagcagacg gaaacaccgc      180

agaaagaaac aucggaucga acgcaucaag agacuuauug uucagcacga ccugcucucc      240

aagaaagagc uggauucucu uuaucugagc ccguuuaaua ucgacgugug gaaccuccgg      300

guggaggccc uggaacgcaa gcuggauaau agagaguucg cccggguccu cauucaucug      360

gugcaacgca gaggauuuca gacgauccgg aaauccguug aaauucagga ggagggcaaa      420

cuucuggaaa acgugucuga gaaugaaaga aucaugaagg ucaacgggua caagaccgug      480

ggagagaugu ucagcaagca cgaaaaauuu acacaucaca agcgcaauaa agacggcucc      540

uauaccagcg uugugacacg gucucuccug cuuaaggaga uuaaaaccau cuucaacucc      600

cagagaaucc ugggcaaugu cuuuagcaca caaaagcucg agucugauua ccuguauauu      660

uggggguccc agcgccccac ccugacguac gaacagcuca ugagcauggu gggaaacugu      720

aucuaugaga aaaaggaaaa gcgggcuccu aaaaccucuu gggccuucca guacuuuucc      780

cugcuucaaa agauuaauaa acugaaggug agcgacgaua acacacucag aaaacugucc      840

aaggaggaac gcgacaucgu cauugagcuu gcuuucaaga aaaagaaugu uucuuuugug      900

gauauccgga aagcccugaa gcucaacgac aacaccaaau ucaaucaccu gacauacagc      960

cauguggucg agaccaagaa gguggaaaaa gcgacguuua ucgagcugaa gggcuaucac     1020

cucauuaaaa agcagcugaa agacagaaac guugaacuua cacaggagcu gcagcccaag     1080

gauuacgacg ccaucgcugc cgcuuccacc uucuucaaga augauauuga auuucgcgac     1140

uaucuccgga accaauaccu ggauagcaau gggaaaagaa agucuaacau cgccaauaaa     1200

guguucgagg acaaggucau ugcggccgug uuugagcuua acuucuccaa aguuugccau     1260

cugagcuuua agucucucua uaaacugauc ccucaccugg aaaagggacu cgauuacaag     1320

gaggcuaucc ugugugccgg cuauaauuuc cgcgacgaaa acaaacggga uaagaaacuu     1380

uuucugcccg ugauucagca ggagacaaau ccugucgugc acagagcucu cacccaagcc     1440

cggaagguca ucaacgccau uauccgcgag uacgggagcc cgaauaccau uagauucgug     1500

guugcuaacg accuggcgaa aacauacaag cagcggaagg aacuggaguc cacgcuuaaa     1560

aagaauagag ccgaaaacga gaaaaucaag accaaacucc ugucuauggg aauuaucaac     1620

cccacaggca uggauaucgu gaaguauaag cugugguacg aacagagcgg cauuugccuc     1680

uauaccgggc gccagaugga gcuguccagc cuuuuuaaac cuggauacuc uaucgucaau     1740

cauauuaucc ccuauaaccg guccuucgac gacacauacc acaauagagu gcugacccuc     1800

acggagacaa aguaugaaaa aggcaacaag auuccuuuug aguacuucgg ggcugaagag     1860

accuguugga augaauacga gaaacgcguu agcaccucua agaagaucca ugauaaaaag     1920

agagagaaac ugcugauccg gaacuuugac auugaauaug agaaugaaau gaucuccaac     1980

aauguguucg auacacacua cauuggaagc uauuucgaga aguacaucga cgaacaucuu     2040

caauuuaaaa aguccaccaa aaagcagaag gucuucucua uucacggcaa caugguggcc     2100

caccuccaua gccgcugggg guuuaauaaa aacuccagag agaucuaucu gaaucacgcc     2160

guggaugcug ccaucguugc gguccugaca gaccagcuca uucggaacau cgcugauuac     2220

uaucgcagag acaauauucu ggagcagaag ggaucucuua aauuccccga accguggaag     2280

uccuuuagca ucgagcugga agcccggauu agcccucaag ugcgcgaucu caucaaaaag     2340

cugaaccugg acucuuaccu ugauguggac auccuccaua uuaagcccgu cuucaucucc     2400

aaaaagccua gacggaaagu gaacggcgag gcccacaaga gcacgauucg cucucugauc     2460

ggcauuaaug agaucgggaa aaccguuaca ugcauuaaga ccagacugga agauaucccc     2520

uuugacgaga acggagacuu ccccauguac ggcaaggaua cagacaaaua uaccuacacg     2580

gcuaucaagg aacgguaucu cgaguuuaau aaagauaagg aaaaagccuu cuccaccccu     2640

cuguacaaac uuaagagaaa cggggagccg ggaaaucaga uuaagagcgu gaagaucauu     2700

gacacacgca acaucgucaa uaaagugaac cagggcaagg ggauugcuua uaauucuaac     2760

aucauccggg uugauguguu uagcagagac cagaaauacu augucgugcc cauuuaccug     2820

cgcgauuuca ucaagggcuc ccucccuaau aagauuauca ccgccgcgaa aggauuuaug     2880

caauggcccg agacagacaa cagcuacacc uuccuguucu cuauuuuucc uaaugaucug     2940

aucaagguug ugcccaaaga aggcaaguuc aucaaagcca agaacgagaa uaaagaaaac     3000

auuaaccuua cggagauccu cggguauuuu aagggacugg acuccagcac aggcgcuuuc     3060

acccuggaau cccacgaugg gucucuccug gcccggggaa uuggcacaaa gaaucuucug     3120

cucaucgaga aauaccaggu cgacgugcug gggagauaua acaagguggg caaugaggaa     3180

cgccaggaua uuaaaaucca gcaaaacuga                                      3210


<210>  3842
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3842
augaacuacg acaucggccu gagaauuggg aucaccagcu gcggaugguc cauuaucaau       60

aaggaucucg agcggauuga agaccugggc gugcgcgucu ucgagaaagc cgaaaacccc      120

gaugggacag cuucugccgc gccuagacgg gaggcucgca agagcagacg gaaacaccgc      180

agaaagaaac aucggaucga acgcaucaag agacuuauug uucagcacga ccugcucucc      240

aagaaagagc uggauucucu uuaucugagc ccguuuaaua ucgacgugug gaaccuccaa      300

guggaggccc uggaacggaa gcuggauaau cgcgaguucg ccagaguccu cauucaucug      360

gugcagcggc gcggauuuca gacgaucaga aaauccguug aaauucagga ggagggcaaa      420

cuucuggaaa acgugucuga gaaugaacgg aucaugaagg ucaacgggua caagaccgug      480

ggagagaugu ucagcaagca cgaaaaauuu acacaucaca agagaaauaa agacggcucc      540

uauaccagcg uugugacacg cucucuccug cuuaaggaga uuaaaaccau cuucaacucc      600

caacggaucc ugggcaaugu cuuuagcaca cagaagcucg agucugauua ccuguauauu      660

uggggguccc agagacccac ccugacguac gaacagcuca ugagcauggu gggaaacugu      720

aucuaugaga aaaaggaaaa gcgcgcuccu aaaaccucuu gggccuucca auacuuuucc      780

cugcuucaga agauuaauaa acugaaggug agcgacgaua acacacuccg gaaacugucc      840

aaggaggaaa gagacaucgu cauugagcuu gcuuucaaga aaaagaaugu uucuuuugug      900

gauauccgca aagcccugaa gcucaacgac aacaccaaau ucaaucaccu gacauacagc      960

cauguggucg agaccaagaa gguggaaaaa gcgacguuua ucgagcugaa gggcuaucac     1020

cucauuaaaa agcagcugaa agaccggaac guugaacuua cacaggagcu gcaacccaag     1080

gauuacgacg ccaucgcugc cgcuuccacc uucuucaaga augauauuga auuuagagac     1140

uaucuccgca accaguaccu ggauagcaau gggaaacgga agucuaacau cgccaauaaa     1200

guguucgagg acaaggucau ugcggccgug uuugagcuua acuucuccaa aguuugccau     1260

cugagcuuua agucucucua uaaacugauc ccucaccugg aaaagggacu cgauuacaag     1320

gaggcuaucc ugugugccgg cuauaauuuc agagacgaaa acaaacgcga uaagaaacuu     1380

uuucugcccg ugauucagca agagacaaau ccugucgugc accgggcucu cacccaggcc     1440

agaaagguca ucaacgccau uauccgggag uacgggagcc cgaauaccau ucgcuucgug     1500

guugcuaacg accuggcgaa aacauacaag cagagaaagg aacuggaguc cacgcuuaaa     1560

aagaaucggg ccgaaaacga gaaaaucaag accaaacucc ugucuauggg aauuaucaac     1620

cccacaggca uggauaucgu gaaguauaag cugugguacg aacagagcgg cauuugccuc     1680

uauaccggga gacaaaugga gcuguccagc cuuuuuaaac cuggauacuc uaucgucaau     1740

cauauuaucc ccuauaaccg cuccuucgac gacacauacc acaaucgggu gcugacccuc     1800

acggagacaa aguaugaaaa aggcaacaag auuccuuuug aguacuucgg ggcugaagag     1860

accuguugga augaauacga gaaaagaguu agcaccucua agaagaucca ugauaaaaag     1920

cgcgagaaac ugcugaucag aaacuuugac auugaauaug agaaugaaau gaucuccaac     1980

aauguguucg auacacacua cauuggaagc uauuucgaga aguacaucga cgaacaucuu     2040

caguuuaaaa aguccaccaa aaagcagaag gucuucucua uucacggcaa caugguggcc     2100

caccuccaua gccggugggg guuuaauaaa aacucccgcg agaucuaucu gaaucacgcc     2160

guggaugcug ccaucguugc gguccugaca gaccagcuca uuagaaacau cgcugauuac     2220

uaucggcgcg acaauauucu ggagcaaaag ggaucucuua aauuccccga accguggaag     2280

uccuuuagca ucgagcugga agccagaauu agcccucagg ugcgggaucu caucaaaaag     2340

cugaaccugg acucuuaccu ugauguggac auccuccaua uuaagcccgu cuucaucucc     2400

aaaaagccuc gcagaaaagu gaacggcgag gcccacaaga gcacgauucg gucucugauc     2460

ggcauuaaug agaucgggaa aaccguuaca ugcauuaaga cccgccugga agauaucccc     2520

uuugacgaga acggagacuu ccccauguac ggcaaggaua cagacaaaua uaccuacacg     2580

gcuaucaagg aaagauaucu cgaguuuaau aaagauaagg aaaaagccuu cuccaccccu     2640

cuguacaaac uuaagcggaa cggggagccg ggaaaucaga uuaagagcgu gaagaucauu     2700

gacacaagaa acaucgucaa uaaagugaac cagggcaagg ggauugcuua uaauucuaac     2760

aucauccgcg uugauguguu uagccgggac caaaaauacu augucgugcc cauuuaccug     2820

agagauuuca ucaagggcuc ccucccuaau aagauuauca ccgccgcgaa aggauuuaug     2880

caguggcccg agacagacaa cagcuacacc uuccuguucu cuauuuuucc uaaugaucug     2940

aucaagguug ugcccaaaga aggcaaguuc aucaaagcca agaacgagaa uaaagaaaac     3000

auuaaccuua cggagauccu cggguauuuu aagggacugg acuccagcac aggcgcuuuc     3060

acccuggaau cccacgaugg gucucuccug gcccgcggaa uuggcacaaa gaaucuucug     3120

cucaucgaga aauaccaggu cgacgugcug gggcgguaua acaagguggg caaugaggaa     3180

agacaggaua uuaaaaucca acagaacuga                                      3210


<210>  3843
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3843
auggccacca gacugggccu cgacaucggg acaaacagca uuggauggug ccuguacgau       60

ggcgacucca uccgggauau uggggugcgc aucuuccuug acggaagaga gcccucuaag      120

cugacgguca gcggcuccgg gaccccucuc gcuguuaaac ggcgcgaugc cagaggagcg      180

cggcgcagac gggaccgcua uauuggcaga cggucugcua ugcugcgcga acuuagaaag      240

cacggccugc ucccggccga ucccaaacag gccaaggugc uggagacaca agacccuuac      300

gcucugcggg ugcgcgcccu cgaugaaaaa cuggagcccc aucagaucgg gagagcucuu      360

uuucaccuga aucagcggag aggauucaag agcaaccgca aggccgaacg ggcguccaaa      420

gccggcgagg acgggaagau cgcuaccgcc acaaaacucc uggaugagga aaugcagaga      480

cuuggagcuc gcacccuggg cgaguuucuc gucaagcggg acucugaguu cgauggggag      540

gccggaaaaa gagugcgcau gcgggcggac agcaaggccu augacuuuua cccugauaga      600

cgccauguuc uggacgaguu ccgggcuauu uggaaugccc aaaaggugug gcaccccgaa      660

cugcucacag agcaggcuug gaacgauauc uuuagaguca uugaauauca gcgcgagcug      720

aaaccucagg ugguuggcca uuguaccuuc gccggcauca auggggugcc ggccgacgaa      780

aagcggcuuc ccaaagcuca cccucuguuu caacagagac ggcucuacga ggaggucaac      840

cagcugaagg ugguggcgcc cggagaagcc ggccgcccuc uuacgagaga ugagcgggac      900

gcucugauuc ucaaacugca ggauaagaaa uccgucagcu ucgaaacccu ggggaagaca      960

cucaagcuga aagacggaga gagauuuaau aaggaaucug agaaccgcaa agcccuugau     1020

ggcgacgagg uucgggccgc ucuguccggg aagaaacucu ucggaaccag augguuucac     1080

uucagcgccg aagaacaaug gaugaucauu gaacgccugc uugaggaaga ggauucugag     1140

acacugcuca ccuggcugaa gaucggcuau agacuggacg acgaagcggc ucucgccgug     1200

gccaaugcua agcugcccga gggccauggg cgguucggac uuuccgccac gacagcucug     1260

cucaaagaac ugaaggccga ugugugcacc uacgcggagg ccgcuaaacg cgccggcuuu     1320

caccauagcg acuucagaac aggggagguc uuugaucagc ugccguacua uggagaaauc     1380

cuugcucggg agauugcccc uggcaagccc gaauacgggg acgagaacga acgcagauuc     1440

ggcaaaauca ccaauccuac cgugcacauu ggacucaacc agcugacaaa gcugguuaau     1500

gcgaucauuc ggcuccacgg caagcccgau uauaucuuug uggagcuggc ccgcgagcuu     1560

aaacugaacg aaaaggagaa aaucgcuuac aauagacaac ucaaggaaaa cacggacgcc     1620

gcucgggauc gcggggccaa acugggaacc augggcguca gagacacagg gcggaacaga     1680

cugauucuuc gccucuggac cgagcugaau cccggagaug cccugaaccg gagauguccu     1740

uucugcggcg aacagaucuc ucagcgcaug auucagaaug gggaggugga gaucgaacau     1800

auuaucccgu auucccaaug uuuugacgau ggcaucggaa acaagguucu cagccacgug     1860

cggugcaaua gacagaaggg cgaccagaca cccugggagc gcugggggca ugauccugac     1920

cgguggucca uuauccagga acaagucgcu caucugcaca gaucuaaaca guggcgcuuc     1980

ggacccgacg cgcgggauag auacgccgcu gaggaagaca gcuuucuucc ucggcagcug     2040

auugauaccc aguaucucac gcgcaucgcc gccaaguacc uggcuugucu guaccccgag     2100

agaggcgagc ggcacguguu cgccguccac gggggaauga cagcgaugcu ucgcagacuc     2160

ugggaccuga acuccauucu gcucgaucau aauugggugg aaaaccugaa uagcaacgcu     2220

ccgaaaaauc ggcuugacca cagacaucac gccaucgaug ccgcuguugu gggcauuaug     2280

accccugggc ugaucgccga gaucgcgaag gcugccgccc gcgcugaaaa acaaaaccuc     2340

gaccggcugu uugaggagcu gcccaugccu ugggcccagc accccaauga uagacgcuuc     2400

cgggacgaac uuagagagaa gauucucgcu gccaccgucu cucauaaagc ggaucacggc     2460

cgccagggaa agccugaacg gggcagagac aucacagccg ggaagcugca uaacgauacc     2520

gcuuauggau uuacaggccu gacguccgac accgggcucc ccgugguugu gcaccgcguc     2580

ccgcugacaa gccuuacccc uaaagacauu gccgaucccg acagaauccc cgauacaacc     2640

cugcggggau cucucuggca ggcuacgcgc gaccugagcg gcaaggaguu cgaaaaagcc     2700

cugcuugauu ucuccagaac ccaugcccaa uuuaagggga uucggcgcgu gagagugcgg     2760

gagggccuga cacucauccc uaucagaaau cacgcuggaa aaccguacaa gggcuaucug     2820

ggggacucua acgcgcgcua caacguuugg cagaugcccg auggacggug gguccaccag     2880

gugauuagca ccuucgaccu ccaucaaccu ggcuuuaucg agcagagacc ccacccugcc     2940

gcuaagaaag ugcugucccu ucgccagaau gaucugauug ccaucgaacg ggacggggcc     3000

cucagacaga uuaugcgggu ccaaaaguau ggacagaacg gccagaucuu ccugaucgag     3060

cauaaugaag cuggcaacau ggccgagcgc aauaaaaaca cagaugaccc cuuuaaguuc     3120

uacgggccug gagcgagcgc ucugagaaau augaaagccc gccaggugcg gauugacgaa     3180

cuuggcagaa ucuuugaucc cgggccgcgg uga                                  3213


<210>  3844
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3844
auggccaagg acuucgagua cauccugggc cucgaucugg gggaaaccag cauuggaugg       60

uccgugguca gacuuaacga gggcguuccc gaacaccugg agcggcucgg ggugcgcauc      120

ugcucugacg gaagagaugc ucagagccau gaaccucugu ccguggcccg gcgcauugcg      180

agaggccuuc ggcgcagacg ggaccgcuau auccacagac ggauucgccu gacagauguc      240

cucgugaaau acgggcugau gccggaggac aucaaggaga gaaagaagcu gcaagcucuc      300

aaucccuauc ggcugcgcuc ugaagcccuu gauagaaaac uggagccuua cgaacucgga      360

cggguucugu uucauaucaa ccagagacgc ggcuucaaga gcaauaugaa gacggaccgg      420

aaagauuccg agucuggcgc caugaagcag gcuauucuua aaaccagagc caagauggag      480

gcuagcgggg cccgcacacu gggagaauuu cucuauuucc ugaacaaaga caagaccucc      540

acacaggagu acgugcccgu ccggaugaga agccacucug aaaagaauaa aaccguguau      600

gagauguacc cugaucgcgc gauguacgaa gacgagguuc auaagaucuu ugagcaacag      660

gaacuguccc ccgaggccca gacagaaauu uucaacacca ucuuuuucca gcggccucuc      720

aaaccggugg agcugggcuu uugugaguuc gaagaugggg agagacgcgc uuauacggcc      780

agcgucgugu uucaaaaguu ccgggcccuu cagcagauua aucagaucua cgugcaagac      840

gacuaugaaa gacgcccccu gaccaaagag gaaaaggaua uucucaaaca cuuucugcuu      900

gaggacuucu cugcccugga uaagaacgga cuccugacau gggcgaaggu ccggacccug      960

cucaaacugc agaagaaaaa guucaaucuu gaauccgaga gacggaaagg ccugaacgcc     1020

gacguuacaa gcuggaagcu cucuaaugcu gccugcuuug ggcgcgagug guucuccuuu     1080

agcgaugcua agaaagaaga gaucguggcc cugaucaccg gagccgaaaa ggaggaggaa     1140

cuuauugcug agcugaaaac gaaguacggc cucuccgacg aaagagcgaa aaacacagcc     1200

aaugugucuc ugccugaugg cgucgcuagc cuguccgcca aggccaugaa gaaacucauc     1260

cccuuccugg aggaagggau gcuuuaugac gaugcuguga agcaggccgg auacguuuuu     1320

agcuccucuu ucaaaggcgu gcugccugag aaguaugaga ccuuuauuga acccgagagc     1380

ggggaauguu ucgacgagcu cccguacuac ggacaagucc ugcggagaua ugugcugggc     1440

gggguuccuu cugaagagaa caaacuuuuu cccgagaagu acuauggaaa gaucaauaac     1500

ccuacagugc acauuguccu caaccagcug cgcaaacuga ucaauagccu cguggcgaag     1560

uacggccauc cccggaaaau cgucguggaa auggcuagag aucugaaacu uuccaaaaag     1620

cagaaagacg ccauugccaa ggagcaggcu cugaacgaac gcgagaauaa gagaaucggc     1680

gccgaacucg agcggcugga cauucgcaac aauuaugaua acagaaugaa auacaagcug     1740

ugggaggacc uuucuaccga ucccaccaaa cggagcugcc cuuucugugg gaagcaaauc     1800

ucccagcagg acauuuuuag cccggaguuc gaaaucgaac accucauccc cuuuucucgc     1860

uccuucaugg auggaagaag caauaaaguu cugucuugcc ggcgcuguaa cagagagaag     1920

ggcaauaaga caccuuucga ggcuuuuggg aacgacucca cgcgguggaa ugccauuaug     1980

ggacgcgugg aaagccugcc cgagaacaaa agauggaagu ucgcguccga ugccaugucu     2040

cgguuugaaa gagacggcga ucuccuggcu cgcaugauga ccgacacaca guauauggcc     2100

cggaucgcua gacgcuaccu uugcugugcc gcgaauccua accaagucua cgggauuccc     2160

ggccagcuga ccgccaaacu ccgggagcau uggggacuga auagcauccu guccgaucag     2220

aaggaaaaag acagaucuga ucaccgccac caugcuauug acgccuucgu gguugcuugc     2280

acagaccggg gcacccuuca gaaguauagc acggagagac gcgauuacgc caagccggag     2340

augccuuccg aaaaagcucc ccacaucccu uuugcugacu uccggcucag cgauauuaag     2400

agaguguuug acgagaucau cgucucucau aaacccgauc gggggaacgc gcgcgccgcu     2460

auucugaagg gaggcacagu ggccaaacug cacgaagaga ccuauuacgg gaugggaggc     2520

gaagggucca agaagggcac cuggaaacuc auccaacgga uuccugugag cgagaucaag     2580

aaagccucug accuggauug ucuuauugac cagaagggaa cagcuaaccc ccugagaguu     2640

cuccuggucg gcgccuccga gagcgaacag gagcagauca ucgcgucuua uuucgcucgc     2700

agagggaccc auaaagugcg gacauacaga gaacgcaaua aggaugugau ucugccguuu     2760

aaagacaagg auggaaaacu uuaucgguac gccgucuacg gcagcaacgc cuaugcugag     2820

aucuacugcc cugacagagg gaagaaugcc ggaaaguggc aaguggaaau uauccccaac     2880

uauugcgcgc accagaaagg cuucauuccc aaguggcgcc acacggaugc ucaugccaaa     2940

cucaucaugc ggcuguacag aaaugacaug guugccuaug agaaggaggg gaagaccgug     3000

aucgcucgcg ucaaaaagau gggcggaaac ggcgugauug uuuuucggaa ucaccugauc     3060

gccaaagaag agggggacag acucuccugg caggcugugg ucagcggaau gcaaucugcc     3120

aaccugcgca agauuuacgu ggauguucuu ggcagaguga ucgacccuaa acggccgaug     3180

acacagaaug aaaccgaugg gcaggacaac uga                                  3213


<210>  3845
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3845
augagccacu ucagacugaa gcagcucaac uacauccugg gccuugaccu ggggauugcc       60

uccgugggau gggcugucgu ugagaucgau accgaagaga aucccacacg gcucauugac      120

gugggcgugc gcacguuuga aagagccgag gucccuaaaa ccggggauuc ucuggcggcu      180

gcccggcgcc aagccagaag cgcucggcgc cuuaucagac ggcgcacaca uagacugcuc      240

cgggcccgca gacugcugaa gcgggaaggc auucuccacg cugacgaucu gaacgccgag      300

ggacuuguga aagagcugcc gaauaccccc uggcagcucc gcguugacgg gcuggauaga      360

aaacuuaugc cuaaagaaug ggcggccgug cugcuccauc uggucaagca ccggggauau      420

cugucccaga gaaagaacga gucugaaggc gcugacaaag agcucggccg ccugcuugcc      480

ggggugcaug agaaucacca ggcucugcaa agcuccgaau accggacacc cgccgagcuc      540

gcgguuaaga aauuccaggc ccagucugga cacaucagaa accagcgcgg cgauuauacc      600

cauacauuug accggaagga ucugcaacag gaacuucacc agcuguucgc ugcccagcaa      660

aaauacggga auccuuuugu gagcgacgga cucaagcagg gcaucgagac ccugcugaug      720

cagcaaagac ccgcucucuc cggggaugcc guccagaaga ugcugggacg cugcacguuc      780

gaaaccgagg aguuuaaagc cgcuaagcau acauauagcg cggaacgguu cauuuggcuu      840

accaaacuga acaaucucag aauccaggag aacggcaaug aacgcugucu gacagcccag      900

gagcggggcc aaauuaucga ccuuccuuac aagaaagcua agcugaccua ugcccaggug      960

agaaaagaac ucucucugcc ggagaacgcc uuuuucaagg ggcugcgcua cggaaaggag     1020

aaugcugaaa cggccgcgcu cauggagaug aaggcuuacc acgccauucg gaaggcccug     1080

gaaaaagcug gccuuaagac agaguggcag gggcuggccu gcaaacccga acuccuggac     1140

gcuaucggaa ccguguuuuc ccuguauaag acagaugagg acauuagcac cagacuucag     1200

ggccaacucu cugaggaugu caucaacgcc cugcuggcgg ggcucaauuu cgacaaguuu     1260

auccagcugu cccuuaaagc ccugagcaag auucucccuc ugauggaaca gggacagcgg     1320

uacgaugagg cucacgccgc ucuguauguu cgcucuacac cccaaccuca gagacuucuc     1380

ccccagauca ccgccgacga aauuuguaac ccgguggugc ugcggacgcu gucccaggcg     1440

agaaaaguca ucaacgccau ugugcgccgg uacggcagcc cugcuagaau ccauaucgag     1500

accggccgcg agguugggaa guccuucaaa gauagacaag aaauucagaa gcggcaggag     1560

gacaaucaca aggaacgcaa acaagccgcu gagaaguucc ucgaauauuu ucccaacuuc     1620

gugggacagc cuaaagccaa ggauauccug aaacuuagac uguaccagca ucagcacggc     1680

caaugccucu auucugggaa gacacuggac auuugucggc ugaaugagaa gggauacgcc     1740

gagaucgauc augcgcuucc cuuuagccgc accugggacg acuccuucaa caauaaaguc     1800

cucgugcugg cuggcgaaaa ccagaauaag gggaaccaga cacccuacga guggcugggc     1860

ggagccagca auucuccuag auggcaggaa uuuguugcuc gggugggcuc cagccgcuuc     1920

caccacucua aaaagcaacg gauucuccug cggcagcuug augaggaagg guuuaaagag     1980

cgcaaccuga augacaccag auaugucgcc cgguuccucu gcaaguuuau cgaggaaaac     2040

augcugcugc uuggaagagg caaacgccgg guguucgcca gcaaugggca gaucacgucc     2100

uuucucagau cucgcugggg acugcggaag gucagagagg aaaacgaucg ccaucacgcu     2160

cuggacgccg ugguuguggc guguagcaca gucgcuauuc agcaacggau caccuacuuc     2220

gugagacagc gcgagaugaa uauuuuugau ggcgaacuca ucgacaagga gaccuccgag     2280

guuagccggc cguauuuccc ccagccuugg gauuucuuua gacaagaagu gaugaugcgg     2340

gucuucggga aacccgacgg cgauccuucu uuucccgagg ccgacacaaa ggaaacccug     2400

ugcacacuuc uggagacgcg ccucuccagc agaccgcagg ccgugcauga guacgugacc     2460

ccucuguucg uuucucgggc ucccacacgc aaaauguccg gacagggcca caaggaaacc     2520

gucaaaagcg ccaagcugcu ucacgagggg aaguccgugc ugagagugcc ucucacacag     2580

cugaaaccca accuccugga aaagaugguc aaccgggaua gagagccuca acuuuaugaa     2640

gcgcugaaag agcgccucgc ugccuacgga aaugaccccg ccaaggcuuu ugccaccgag     2700

uuccggaaaa agggcgggca gcuggugaag gcuguuagag uggaagauac gcagaaaucu     2760

ggaguccugc uucauaaggg caccggggug gccgacaacg cgacaauggu ucgcguggac     2820

gucuuugaga aaagcggcaa guauuaccug gugccgauuu acuccuggca gguugcccaa     2880

ggaauccucc cuaauaaagc ugugauucag ggcaaggaug aaaagaacug ggacgagaug     2940

gaugaaaccu auacauucaa auuuucuaug uaccccaaug accuggucga gaucaacacc     3000

gggaagcaga gacucuucgg auauuacauc ggccuggaua gagccacggg ggcuauuagc     3060

auccgcgagc acgaccuuga uaauuuuaaa ggaaagaacg gcauuuaucg ggugggggug     3120

aaaacagcca ucuccuucaa gaaauaccag accgacgaac ugggcaaggu cguugugccc     3180

uguagaccua agaaacgccu cccggugaga uga                                  3213


<210>  3846
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3846
augagacugg gccucgacau cgggaccaac agcauuggau gguggcugua ccagacagau       60

gacggcacga ucaccgaugu gcuugacggg ggaguccgga uuuucuccga uggccgcgac      120

ccccaaucua aggagagccu ggccguugau agacgggugg cucgcgccca gagacggcgc      180

agagaccggu aucuccgcag aaaagcggcu cugaugaagc ggauggccgg ggccggacuu      240

augccuacag aucaggcuca cgccaaaaag cuggaauccc ucgacccgua cgcucugcgc      300

gccgagggcc uggauugcga acucccccug acccaucuug gcagagcgcu guuucaccuc      360

aaucagcggc gcggguucaa aucuaacaga aagacagacc ggggagauaa ugagagcggc      420

aagaucaaag acgccaccgc uagacuggac ugggccaugc gcgaugcuaa ggcccggaca      480

uauggggaau uucuucauac cagacaacag aaagcggacg auccuaagcg caugcccacg      540

gugcggacca gacugacagu cgcccgccgg gacaacgcug agaaagagga agccggauac      600

gauuucuauc cugacagacg ccaccucucc gaggaauuug augcucugug ggccgcccag      660

gcugacuacc augaggcgcu gucugaugac cuccgggauc agauuaagac caucaucuuc      720

caccaaagac cccugaagac accugaggug ggccuuuguc uguuuaccga cgaacgccgg      780

auuccgagcg cccacccccu caaucagaga cggauccugc uugagacggu uaacgcucug      840

cgcauugugg ccagagggga agccgcucgg agacucacac uggaggaacg cgaccagauc      900

guccaugccc uggauaauaa aaaccacacc aagacacucu ccggaauggc gaugaaacug      960

aaggcucuug gcaaagugau ucggcugaga agcgaccaga ccuucacccu cgagacagcc     1020

aaucgcgaug ccaucgcuug cgaccugguu agagccucuc uuucccaucc ugaucggaug     1080

ggcggggugu ggacgacccu ggacgaggga gcucaauggg aagucgugca gcgcauuaga     1140

gccgugcaga gcgauucuga guuugacgcg cucgucguuu ggcuggaaca aaaccacggc     1200

cuggauaaug cccacgcuca ggccacagcu aacgcgcccc ucccugaggg guauggacgg     1260

augggccuga uggccacccg cagaauccuu gccgcucugc aguccgacgu gaugcccuac     1320

agcgaugccg uggcugccug uggguggucu cauuccggag gcccgacagg cgaaguccuc     1380

gagaagcugc cuuacuaugg gaaaauccug gaccggcacg ugauucccgg aaccuacgau     1440

gagaauaacg aagacguuaa gcgcuauggc agaaucacga auccuacagu gcauauuggg     1500

cuuaaccaga uccggcgccu cgucaacaga auuaucugca uccacggaaa gcccgacgag     1560

auugugguug aacuggcccg ggaucugaaa aauagcgagg accaaaagag agaaauccag     1620

aaaauuauca agaaaaacac cgaugcugcg auuaagcgcg gcgagaagcu caucaaugag     1680

cuggggcaga aagacaccgg cgccaaccgg cuugugcuga gacucuggga agaucuggga     1740

caugacguca ugacacgcaa uugucccuac uccggcaagc ggaucucugc ugggaugcug     1800

uucgauggaa gcugcgacgu ggaucacauu cuuccuuauu ccagaacccu cgacgauggc     1860

aucgccaaca aaacacugug ucugaaggag gccaaucgcc agaaaagcaa caauacgccg     1920

ugggaagucu ggggagagac cgagcaaugg gacacaauug cugccaaccu caagaaucug     1980

cccgauaaca aggcguggcg guuugcuucu gacgccaugc agagauucga aggggagaau     2040

gacuuuuccg cccgcgcucu uaaagauacc caguaccuga gccggaucgc cagagcguau     2100

cucgacgcuc uguacgaugg cggggacggc aacucucacg uguggguugu gccuggacgg     2160

cugacagaaa ugcuucgcag acauuggggc cucaaugggc uggaggcccu gaccgauagc     2220

gacgaaaaca cggucaaggc caaaaaccgg auggaucacc gccaucacgc uauugacgcc     2280

gcugugguug ccgcgaccga uagaggacuc auccagcgga uuuccaagau cgccaaaaga     2340

gacgagcaag cuggcgccga ggaaguggcu cgcucuguca gcccucccug ggauggguuc     2400

cgggacgaca ucgccgccca gcuguccaga auuaucguga gccaccgcgc ugaucaugga     2460

cggauugacu cucaggcgaa gcagcaaggc gaggaugcca cagcugggca gcuucacaug     2520

gacaccgccu acggcguggu ugaugccgga agagucgugu cccgcaagcc ccugcucagc     2580

cugucugaau ccgacaucaa aaagauucgg gauccggacc ugcagagaca ucuuacacgc     2640

gugaccaaag gccucgauaa gaaagaguuc gaacacgcuc ugagcgacuu ugccaaugcg     2700

agaaagcugc cugaucaauc cgagaacccc uauuucggga ugcggcgcgu cagacucauc     2760

gacgcucugc aggauucugc ccgggugagc guucgcgaca gagacggaca uaucuacaaa     2820

gccuauaagg cuggcuccaa ucacugcuac gagguguggc ggcuuccuga ugggaagauu     2880

aaacccuggg ccaucucuac guuugaagcg caccagacag gaaccgagag caagccucau     2940

cccgcugcca aaagacugcu ccgcgucuuc aagcgggaca ugguggccau ugaaagagau     3000

ggcgagacac ugauguguua uguucagaaa auggacaucg cuaacgggcu guuucuugug     3060

ccgcacaccg aaggcaaugc cgaugcucgc aaccgggaca aggaugacuc cuucaaguuu     3120

auucaaauga gcgccgucuc ucugaucaga gcgaaaaucc ggcgcauuca uguggaugag     3180

augggaagac uccgggacca gggcccuccc cgcuga                               3216


<210>  3847
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3847
augagauaca ccaucggccu ggacauuggg aucgccagcg ugggaugggc ugucauuaac       60

gaggauaagu cccggaucga agaccucggc guucgcgugu ucaaaaaggc cgaggaaccc      120

gaugggaaau cucugaaucu ugcgagacgg gaggcucgcg gaagcagacg gcgcauuaga      180

cggcgcgcca caagaaugca gaaggugaaa gaacugcucg ucaaguauaa ccuggugucc      240

gagaaggagc uugaaacgcu guacaucauc accgagacaa ccccugacgu uuaugaacuc      300

cgggccaaag gccuggaucg ccugcucucu agacaagagu uugcucgggu gcugacaaau      360

auugccaagc gcagagggua caaaagcaac cggaagaucg acgaggaaga uaaagagguc      420

ggaaaacuuu ccaagggcac cauugaaaau aaaagaaucc ugcaggagaa gggcuaucgc      480

acaauugggg aaauguucua caaagacgag aaguuugagu auaacaaacg gaauaaggcu      540

ggagaauaca acucuaccgu gcucagaagc augcugaucg augagguuca cacgcuuuuc      600

gaaaugcagc gcaaauuuaa guccgaguuc gccaaugaag aguuugagaa ggaauacgug      660

gaggucauga ccuaucagaa accguacauu acacccgaac ugcucgagaa gaugcugggc      720

aaaugcaccu ucgaaaaggg ggagccucgg gcgcccaaaa acagcuauac auuugagaga      780

uucaugcugc uccaaaaggu gaauaaccug aauaucuaca ucaacggaga aaagguggag      840

auuccucagg aaaagaagaa agagaucauu gagcuugccu auaagcaguc ugaaaucaaa      900

uacacccagc ugcgcaagaa gcucgagcug cccauugaag cucgguuugc cgaccuuaau      960

uaucaaauca aaagaacgga ggaugaaucc gaggaggcuc uggucaagaa aaucgaagac     1020

aagaaauucg uuaagcucga gggcuggcau aagauucgcc uggcccugaa agaaaacaag     1080

gauaaauuug agaagaucga gaaaaacccu gaacuccaga auauuaucgc ggacgcccug     1140

gugcggaaca agacagacga gaccauuaaa aauuaccuug aaaagagcga uaucgacgag     1200

gaaaucauua acgcuauccu gaauauuaac uucacaaagu ucgggcaccu cucuuacaaa     1260

gccaugaaua agaucauucc ggagcuggaa aaaggacuua ccuaugauaa ggcuugugag     1320

aacgugggcu acgaguuuaa aggcagaaag aaugaacugc aguauaagcu gcccccuguc     1380

accgagcucg aagacgagau ccugaacucc gugguucucc gcgccgugag ccagacacgg     1440

aaagucauua augccaucau cgauaaguac gggucuccca cggcuauuua uaucgagacc     1500

gcgagagaac uguccaaacc uuacgacgag cggaugaaga uugaaaaacg ccaaaaggag     1560

aacgccgcua auaacgauca gaucaaggaa uauauuaaag agacauucgg auuugacccc     1620

aagccguucg auaucgugaa aaugaaacuu uggagagagc agaauggcaa augcgccuac     1680

agcuccaagc cuauccccgc cgaacggcug uacgaggaaa acuuuguuca aguggaccau     1740

auuaucccuu ucucuagaug uuuugaugac agcuaucaga acaagguccu cgugcugacc     1800

gacgagaauc agaaaaagaa agaacgcaca cccuucgagu acuuugggga ggauaaggaa     1860

cgguggcacc aguucgagga auuugucaac cugaccuaua aauucaauac gagaaagaaa     1920

gagaaccuuc ucauuaagaa uuuuaacgaa gagaagucca aagaguggau ccaacgcaau     1980

auuaacgaca caaaguacau cagcaaauuc auguauaauu acaucgcuaa caaucugaag     2040

uucgccgauu cugaauccaa aagaaaggug uauaacauua auggacaggc gaccagcauc     2100

cugcggcauu acuggggccu caagaaagac cgcgaggaau cugauaagca ccacgcucag     2160

gacgccguua uuaucgccug cgcuaccaac aaaaauauua agaaagugag cgauuacucc     2220

agacggaagc ugcuuuauaa gaacaacgag aucaucgacg aggaaacagg ggagauuauc     2280

gauaaaaagu acaauauuga caucucuauu aaagaacccu ggccuaaguu ucgcgaggaa     2340

gucgaggcca gaauggagga ugcugacaac aauggacagc uguaugcccu caucaacggc     2400

aauuucacca acuacgauga cguggacauu acacauauca aaccgaucuu uacgagccgg     2460

augcccgaac gcaagauuac cggcagagcg cacaaggaua caaugcgguc ccaaaaauuc     2520

auccagcagg ggcagaauuu uaccguugug aagaaaccuc ugaaggccau uagcaaacaa     2580

gagaucgaag gaauuaucaa caaucccgac uucaagaacc uguaucuuuc ugauaagaaa     2640

cucuacgacg auaucuauca gaagaugaaa gaggcugacu uuaaggccga aaaggcuuuc     2700

gccaaugagu accgcaagua cuccaaaaac ggcaauagcc cuauugucag aucuaucaag     2760

guggcguccg ccggggcuac aggagugcag cugaagaacg gcgccauugc ugagaaugcc     2820

aacaugguuc gggucgaugu guuugaaaaa gacaacaaga auuaucuggu gcccaucuac     2880

gucagcgauu ucgccaaaaa cgagcucccg aauaaggcua uuugugcgaa caaagacgaa     2940

aaggagugga ucgaaaugac cgagaaguau aaauuuaagu ucucccugua cccuaaugau     3000

cuugugaaaa ucaagcgcaa aaacgagaag gaauuuuaug ccuacuauug cucuacgcau     3060

agagggaccg gcaauauuaa ccugcuccug guuaauggag aggcucggaa ggacggcgug     3120

gggaucaaaa acacagaaau uuucgagaag uaccaggucg augugcuggg aaauaucagc     3180

aaaguuaaca aggagaaaag agaaggcgug aaguga                               3216


<210>  3848
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3848
auggacuaca gaaucggccu ggauauuggg accaacagca ucggaugguc cauuaucgag       60

cucacaacgg ugcagucuaa gcuggacggc aaauaucggc uuaaggccgu cgggcugauu      120

gaugcuggag uucgcauguu cgacagagcc gaagugccca aaaccggcgc gagccucgcu      180

cugccucggc gccuugccag auccucucgg cgcagacuga cacggcgcag cgccagaaag      240

aaagcuaucc ggaaucuccu ggugaaggag gauauccuga accaaauuga acucgaccac      300

cuguaccgca ccuccgagcu ugucgauauc uggcagauua gaguggaagg gcuggagcgg      360

cgccucggaa gacaggagug ggcccggcug cuuauccauc ugggccagaa gagaggcuau      420

cgcucuacac ggaaagcuga agagggggcc gacaccgaag gcagagcggu ucucagcucc      480

auuaaggaga aucaagagac acugaaacag uaccgcaccg ugggagaaau gugggccacg      540

gaugacaagu uuaaagggcg ggucagaaac agcaccaaug aguucaucuu uaacgugucu      600

cgcgauaauc uggaagagga aauucgguuc cucuuucagc agcaaagaga gcugggaucc      660

auguaugcua gcaagcugcu ggaggaaucu uacauggagg uuuggggcca ccagcucccg      720

uucgccuccg gggaaagcau ccugaagaac gugggaacau gcucucuuua ugagaaagaa      780

aagcgcgcug ccaaagcgac cuacacauuu caguacuuca uggcccugga caagcucaau      840

aacguccgga uccugcccuc caugagaacc cugagcgagg aggaacgcgg ccucgcucug      900

aaagccaugu uugagcggga uaaacuuccu aaaaaucccc cuaaguucac guauucccau      960

cugagaaagg cuauuaaacu cgaagaggac gaaacauuuc agaccaucuc uuacgacgug     1020

gagaagacac uggccaccaa cgagagcgcc guguucguca aucuugcugc guauuaccaa     1080

auuaaaaagg uuugugauga acacaacgag aauuaugacu acguggauua uaacaccauc     1140

ggccaugccc ugacagugua caaaacggac gaagauauua acaaguaccu cgcuuccaag     1200

aauaacaucg ccaaacgggu cuaugccaau gacuuuaucg auagcgugcu gaagcacucu     1260

uacaccacau ucgggcaccu gucccucaaa gcucugagcc gcauuauccc ccuuauggag     1320

gagggagaag uuuucgccaa ggcgaaagag aaggcuggcu uuaaggaaga gucuuaucug     1380

gaaauuaaaa gcaagcuccu gaccaacauc ccugacgcca uuaaaaaucc gguggucaug     1440

agaucccuga cacaggcccg gaaggugauc aacgcuauca uuaaaagaau gggggagcag     1500

cccgccucua uccaaauuga gcuugcucgc gaacucagca aggauguuuc cgagcggaag     1560

aaaaucacca auggauucgc cgaaaacaga agccgcaauc aguuugcgau uaagacgcug     1620

gaggaaaacc auaucacaaa uauuagaggc caggacaucg ugaaauucaa gcuguggcag     1680

gagcaagggg gacggugcgc cuacucucuc aaaccuauca agcuggagga acuuauuucc     1740

uuucugaaaa aggagcgcag auaugaacac aaccucgaug ucgaccauau cauucccuac     1800

agcaagaccu ucgacgauuc uuauaccaau aaagugcugg ucuuuuccga cgagaaccgg     1860

aagaaaggcg aucagauccc ugcugaauac cugggcauug ggacaaagcg cugggagaaa     1920

uacaaggagu ucgugaucac cagcaaguuu cccgccucca aaaagaaaag acuucucaag     1980

gaacagcugg uugagcggga ggaacuguuc augaaagagc gccaccucaa ugacacaaga     2040

uaugcuucuc gguacuuuau gaacuuccug cgcgaaaacc uucugagaaa ggagacggug     2100

cuccacgaaa cccagugggu cacagccgug gcgggacggg uuaccagcca uaucagaaag     2160

cgccugggcu uugggaaaga ucgggaggaa acacaccuuc aucacgcccu ugacgcuauu     2220

aucguggccu guaccgauaa guggaugauu gacugcguca cgaauuauua caaauccaga     2280

gagggaggcu cuaucaagcg caaagagauu cgguucccga gcccuuggaa cggguucugg     2340

caugaugugc aaaccaagcu cgcucagcug ccccagccug aagugaucaa ugaguccauc     2400

agcaagggca accccgacgc cguugauuau augcuggucu cuagaaugcc ccgcaaaucc     2460

auuacaggag ccgcucacaa ggaaaccauc agcaaauggg gcggguacga cgauaaggga     2520

aaagcgcucc uggugaagaa aguggcccuu ucugagcuga aguuuaagaa ugacgacuuc     2580

ccuauguaua acaaagaaac agauccgcuc cuguacgagg cuauuaagaa ucgguacguc     2640

gaguccggca gagacgugaa acgcgccuuu gccacgcccc uguauaagcc uagcaaaucu     2700

gggaagcgga acgaaguuaa gagagugaaa gucaucgcug agaagaaagc cauggugcgg     2760

gaaguuaaug gaggcauugc ggagaacggg ggccuugugc gcagcgauau cuuccucaag     2820

gaaaagaagu acgucauggu gcccguuuau accuuugaca caaucaagca gcaacugccu     2880

aauaaauacg uggucuccgg aagaggcuau gagcaguggc ccgagcugac cgcugaacac     2940

guguuccagu uuagcgugua cccguaugag cucguccaug uugggacaaa caagggagau     3000

aauucuuucu acuacuuugc cucccugaac auuagcucca augugcuuaa auucucugaa     3060

uguaaccggc cuaccaagga cuauaucgag auuggccuga acagcaucau ucuccuggag     3120

aaauacgaaa ucgaugugcu ggggcgcauu accggacagu ccaagaaggg caucagacaa     3180

ucuuuuaaaa auaacaagag ccagaugaaa gaguga                               3216


<210>  3849
<211>  3219
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3849
augagacugg gccucgacau cgggaccaac agcgugggau gguggcugua caagacagau       60

ggcgaggccg cuuccgcccg gauugucgaa guuaucgacg ggggagugcg cauuuucucu      120

gauggcagag accccaaaag caagacgucc cuugcggugg aucggcgcgu cgcuagagcc      180

gcccggcgca gacgggaccg cuaucugaga cggcgcgcuu cucucaugaa acugcuugcc      240

agaaccgggc ugaugccugc ugauccggug gaggccaaga aacucgaacg gcuggacccc      300

uacgagcugc gcagcacagg acucgaugac gcgcugccuc uuacccacuu uggcagagcc      360

cuguuccauc ucaaucagcg gagaggcuuu aaguccaacc gcaagacaga ucggggagac      420

aaugaaaacg gaaaaaucag agaggcuacc gcccgccugg aucuugagau gaugacaucu      480

ggcgcucgga gcuaugggga guuccugcac augagacgcc aacgggccuc cgaccccaga      540

cauguuccua gcgugcgcac ccggcucucu gucgcggcca gaggaggccc cgacgcuaag      600

gccgaagcug gguacgauuu uuauccugac cgccaccauc uggaagagga guuccacgcc      660

cugugggccg cucaggcgac gcaccaucag gcccucaccg augaccugcg ggaucuugug      720

uuugagaaaa uuuucuacca gagaccgcug aaggaaccca aaaucggacu cugccuguuu      780

acagcugagg aacggaucgc caaggcccac ccucuuaccc aacgcagagu ucuguucgaa      840

acaauuaauc agcuccgggu gaccgcugac ggcagaucca cgcgcccccu gacacuggag      900

gagcgggaua cccucgucca ugcccuggac aacaagaaac cuacaaagag caugaccucu      960

augaauauga cccuuaaagc gcuggcuaag cuccugaaac uuagagaugg cgaacgcuuu     1020

acacuggaga cgggggugag agaaaacauc uccugugacc ccgugcgggc cugcauggcc     1080

cacccggcuc gcuucggacc ucaguggagc ucucucgauu gggaggccca gugggacguc     1140

auuuccagag uucggcaagu gcagagcgaa ggcgaguuua ucgagcugac cgacuggcug     1200

auucaggggc acggacucga ugcugaccau gccaaggcgg ugggcaaugc cccgcugccu     1260

gaaggguaug cucgccuugg auucuccgcc acagagagaa uccuggauca gcucaaagcu     1320

gaagucugua ccuacucuga cgccguggcg aagugcggcu ggcaccauag cgaucaacgg     1380

acaggcgccu gucuggaggu ucugcccuac uauggggaag ugcuugaccg ccacgucauu     1440

cccggaaccu acgaugcuac ggacgaugag aucacaagau auggccggau caccaacccu     1500

accgugcaua uugggcucaa ucagcugcgc agacugguua acaagaucau ugaggugcac     1560

ggaaaaccgg accagaucgu cguggaacuc gcccgggagc ugaagcaauc cgaaccccag     1620

aaaaaggaug cuaugaaaag acuucgcaau acaaccgccg acgccauucg gagaagcgag     1680

cagcuggagc agcucggcca accuaacaca gggcacaauc gcaugcugcu gcggcuuugg     1740

gaagaucucg gccccgcuau cggaccuaga ugcugucccu acacgggcaa gaccaucucu     1800

gucacaaugg uguucgacgg guccugcgac guugaucaua uucugccguu uagccgcacc     1860

cuggacgauu cuuucgcgaa ccggacacuc ugucugaagg aggccaacag agaaaagagc     1920

gaccgcaccc cuugggaggc uuggggaggc acggaucagu gggacgauau cgccgccaau     1980

cuuaagaacc ugcccgagaa aaagcggaaa agauuugggc cugacgcuau ggaaugguuc     2040

gagggagaaa augauuuucu cgaccgggcc cugguggaua cccaguaucu ggcgaagauu     2100

ucccgcaugu accuugacac acucuucacc aagggcgggc acgucugggu gguucccggc     2160

agaaugacag agaugcugcg gcgccauugg ggacugaacg cucuccugaa ugauaaagac     2220

agaggcgccg ugaaggccaa aaaccggacc gaccacagac accaugcuau cgaugccgcg     2280

gucguggcug ccacggacua ucgccuuaug cagaagauuu cucgggccgc ugggcaagga     2340

gaaaaugccg gccagagcgc ugagcugauc gccagagaua cagacgcgcc uugggaacac     2400

uuucgcgccg auaucgcuuc ccagcucgag aaaauuaucg ugagccaucg ggccgaccac     2460

gggagaauug auguugaggg acgcaagacc ggccgggacu cuacagcugg gagacugcau     2520

aacgauaccg ccuacggcgu caucgacgau cugaccgugg ccucccgcac accccuuaug     2580

agccucaagc cgucugacau ugcuaucacg gaaagaggga aaaauauucg ggacccucag     2640

cugcaaaacg cgcuguacgu ggccaccaag ggcaaagagg ggaagauguu cgaacaggcu     2700

cucgucgauu uugccuccaa agagggaccc uaucagggca uccgcagagu gcggcugguu     2760

gaaacacuuc agaaggccgc ucgcguggag aucgggaaaa ccgagagcgg acaaccccug     2820

aaggccuaca agggcgacuc caaucacugc uaugaacucu ggaaacugcc ugaugggaag     2880

auuguccccc aggugguuac aaccuacgag gcgcacgcug gcucugcccc cagaccucau     2940

cccgccgcua aacggauccu gagaguguuc aagaaagaca uggucaaacu uacgaaggau     3000

ggagaagaga uugugggcua uguucagagc augcacauca aaaacgggcu guuuguggcc     3060

caacaucucg aggcgaaugc ugacgcccgc aaccgggaua agacagaccc uuucaaauuu     3120

cugcagaugg gagccggcac ccucgucaag accgggcuga gacgcgugcu uguggaugaa     3180

auuggacggc ugagagaccc cggcccuccc aaacucuga                            3219


<210>  3850
<211>  3225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3850
augcagacca agaaccugcc cuacacacuc ggccuggaca ucgggauggc cagcguggga       60

gcugcccuuc ugcucccuga ucaagagcgg auucuggcgc uucacgucag aacguucgac      120

aaagcugaaa ccgccaagga gggcgaaucc cugaauaaaa uccgcagaga uucucggcuc      180

acacgcagac ggauucgcag acgggcccau cgccugguua gacuggcucg gcucaugaag      240

cgcguggggc ugaucgccga cgcuaacccg gaggccuuug cgcuucccgg agugagcccu      300

uggcaccuga gagccgaugg ccucgaccgg cugcuugaac ccaaagagug ggcugggguc      360

cuguaucauc ucgugaagca ccgcggauuc caguccaaua gaaagucuga ggccaaagcu      420

gaugaaaagg ccggccagau gcugagcggc guugggcgga accaggcgcu gcucaaagag      480

gccggauaca gaaccguggg cgaacuggcu gcccgccaug aggacuuuaa ggaggcuaaa      540

cggaauaaag ggggauccua uucucacaca uucgccagag ccgaucuuaa cgcugaacug      600

aaucuccugu uugagcgcca acgggcgcuu ggcagcagac uggccuccgc ugacuuccag      660

gaaaacgucc gcgaucuccu gauggcccgg agaccuaccc ugagcggggc caaucuccug      720

aagauggugg gaaagugcac auuugagaag ucugaagacc gcgcucccaa agccggcuac      780

cgggcggaga gauucguuug gcuuggcaag cugaacaauc ucaaaauugu gggguccgga      840

gaugcucgcc cucugaccga cgccgagcac cggaucguca ucgaccuucc guuuacgcag      900

gccaagcuga ccuucaaaca ggugagaaag gcucucgaac uggagacaca ucaacgguuu      960

aaccugcuca gcuaucgccc cgauccuaag ggcaaagaca aggaucccga agagucuacc     1020

uucuuugaag ccaaauccuu ccacacacug agaaaggcuu acgagggggc cggacuuaaa     1080

aaggaguggc agcgggacgc gcuggauccu gacagacucg augcccuggc uuaugcccug     1140

accuguuaca aggacgauac ggaaagccgc caguggcuug uggcucaggg cauugggaca     1200

cccaucauug aggccguccu cgcggaaucu uucgacaaau uuauccaucu gucccaaaag     1260

gcccugaaag cuauucuccc gcacauggag gccggacagc gguacgauga cgcugccaag     1320

agcgccggcu augaccacuc ccagccugau gcuggcaccg ggaaaagagc gacacugccc     1380

ccucccgaca aggauaccau ccgcaauccc guuguguaca gagcccuuaa ccaagcucgg     1440

aagcugguga augccauugu cgccgaauau ggaccuccgg cugccgugca uaucgagcuc     1500

gcgcgcgacc ugucuaaacc caucgaugag agacgggaca uugaaaaggg ccagaaagag     1560

uuccaggcuc ugaaggaaaa acuugccgcc gcuuuugagg agaccuucca ccgcaagccu     1620

agcgggcucg aucugcugaa guacagacuc uuucgggaac agcuggacca augccccuau     1680

ugucagaaag cccuuguugu ggaucgccug uucgagccug gauacgcgca gaucgaccau     1740

gcucuccccu auucccggag cuuugaugac ggcaugaaca acaaggucgu gguucacgug     1800

gccugcaauc aggauaaagg gaacagaaca ccguacgagu uccuggacgg cgccggagac     1860

ucugaacgcu ggcaaagauu ugucgcuugg gugcagggca auaaggccau ucggcaggcu     1920

aaaagaaacc gccugcuucg ggucaauuuc gggacggaug aagccaagga guuuuccaaa     1980

agaaaccuca gcgacacccg cuacaucugu cgggaguuca agagaacaau ugaaacccag     2040

uuugcguggc auucugaugc cgagggaaau gaacgcugcg ugguucugag cggccaacug     2100

acaucccucc ugcgggcuag augggggcuu aucaaggugc gcgaggacgg agaucugcac     2160

cacgcccucg acgucgcugu gaucgccgcc gcuucucgga gccugguuaa aagaauggcg     2220

gauuauuccc ggcgcaacga acuggagaug gugagaaauc gguacgucga cccugccacc     2280

ggcgaggugg uggauguugc ugcccuucgc cagauugaag accauuuccc caagccuugg     2340

ccccucuuca gagaggaacu gcuggcccgg cucgcuccug agcccaaacu gcagcuuacg     2400

ugugucagcu cuuaugauga agggcugaga gaguccaucc gcccgauucg ggugagcaga     2460

gccccuacac gccggggccu cggagcggcu caccaggaga ccaucagauc ucugcgcgac     2520

ggcucccaaa gcgccauuaa gacaccccug cgggaucuua accugaaaga ccucgaaaau     2580

aucguggggu uugagagaga caccgcccug aucgaugcua uucgcaccag acucgaagcc     2640

uucggaggcg aggggaagaa ggcguuugac gauaaacagc ccccucugcg gaagccgucc     2700

aaagaccccg auaaggcucc uaucauucgc ucugucaaac uucuggugac acaaaagagc     2760

ggaaucccca uuagaggcgg gaucgccaac aauggcgcca ugcuccgguc cgacauuuuc     2820

acgaaggcug gaaaauuuua cgccguuccu aucuaugugg cugaugccgu ccgcggcgag     2880

cugccccaua gagcgguggc ccaggcuaag ccugaacugg aguggcccau gauggacgaa     2940

ucuuaccagu uccuuuuuag ccugcacccg aacgauugga ugaccauccg gcucaaaaau     3000

gagguuagag aaggguauua cgccggacug gaccgcgcua caggcaacau uucccucugg     3060

cugcacgauc ggaaccagaa uguggggcag aagggacuuc ugaccggcaa cgggaucaaa     3120

acagcccucg ccgucgagaa guaccaugug gaccugcugg gcaaacuuca cagaguucau     3180

aaggaggaac gccaaccuau ucacaucaag cccggaaaac gguga                     3225


<210>  3851
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3851
augaaguaca gcaucggccu ggacauuggg aucgccuccg ugggaugggc ugucauuaac       60

gaggauaucg aaagaauuga ggaccucggc guucggaucu ucgauguggc cgaacagccc      120

aaaaccgggg cgucucugag cacaccucgc agacuugcuc gguccacgcg caagagacug      180

cggcgcagac ggcaccgcgu ggagagaguc cggaaauuua ugauccaaaa gggacuccug      240

acccaggugc agauggaaca gcuuuaugac uggaaagaug gcgaccugga uauuuggcuc      300

guucgcguga augcccugga gagaaagcug ucugaccggg aguucgcccg cauccucguc      360

cauuuugcua agaacagagg guuccaaagc aaucggaaau ccgaacugca ggaggaagau      420

aacggagccg ugcuuucugc uguucaggag aauaagagac ugauggagga aaaaggcuac      480

cagacaauua gcgagaugcu ccuguaugac gaaaaguuug agggcaccaa acgcaacaaa      540

gggggagaau acacacacgu ggucgcccgg uccgaucuua agagagagau ccaacugauu      600

uucgagaagc agcgcgcgua uggccaugcc uuugcuacca uggaaaauga ggacaaguuc      660

cuccagaucu ggagcucuca gcggccguuu gccacaaaag augacauugc uaagaaaauc      720

gggaacugca ccuucgaacc caaggagaaa agagccccua aguccacgua caccuuugaa      780

cgcuuccggg cgcuggacaa gcugaauaga cuccgcaucc ugagcggaac aucucccgcc      840

cggucccuua cccaagagga gagagaucug auucucaaca gccuguuuuc uaaaaaggaa      900

gugaaauaua aggagcuucg caaacugcuc aagcugaaug acgaacagcg guucaacgag      960

aucuucuacg auccuaauga aaagauugag aaaaacgaga auagaacauu ucugucccuc     1020

gaaagccagu acaagauccg gaaaauucug caaaagaucg agggcaaauc ccuuaacgac     1080

accuaucgcc ccguggaucu ggacacgauc gcuuacgccc ucacagucuu caaggaugac     1140

caggauauua gagacuaucu gcagaaccag uacguugcuu cuaaugggaa gaaagaacuu     1200

aaccuggcca aucggaagua uaacgaugag cucaucgagg aacugcugca ccucagcuuu     1260

accaaauucg gacaucuguc ccuuaaggcc cugagaaaua uucucccuuu uauggagcaa     1320

ggcaucccgu accaccaggc uugugaaaaa gcgggcuauc aguucaacaa gaaggugaau     1380

cgcgagaaac agaagcugcu gcccccugug cccgccaaag acauugucaa cccugugguu     1440

auccggagcc uuucucaagu gagaaagguc aucaauggga uuaucaaacg cuacggaucc     1500

cccagcucua uuuacaucga acucgcuaga gagaugggcc ggggguauaa ggagcgcaaa     1560

gaccuggaaa agcaguacaa caagaauaga cagaugaacg agggagccaa aaagcacauu     1620

cuggaacucc ggccggaguu uggcgauccu cgcgggcaug acauccugaa auauaaacuu     1680

uggcaggaac aagauugcag augugccuac agcuucaagu ccauucccau caagcagcug     1740

uuugagaucg gauaugcuga gguggaccac auuaucccuu acucucggag cuucgaugac     1800

uccaauaaca auaagguucu cgugcuggcc gcggaaaacc agaacaaaca gaaucgcaca     1860

cccuaugagu gguuuggcca ugaugaaacc aaguggcaag aguucaccag ccuggucaca     1920

acgaugaaag ugucuaagaa aaagaagucc cuucuccuga aaaagaacuu ugacgaagaa     1980

caggcugagg aguucagaag ccggcaccug aaugauaccc gcuacauuac aagauacauc     2040

aaaaccuuca uugaggacaa ccuccaguuu cggaucgaag agggcaagaa aacauauguc     2100

cacaccguga auggggccua cacgucucug augagaaagc gcuggggauu caacaagaau     2160

cggcaagaga acgcccuuca ucacgcucug gaugccguua ucguggcugu cucccucccc     2220

uuuaaacaua aggugagagc cuauuucaaa cgccgggaag aguacgcgca caaucagcug     2280

aagagagaag gcgaguuuuu cccugaaccg uggcccgggu uuauucauga gcuggagauc     2340

cgcaugaugc aggacagcga aaacauuaaa cuugccaugg agucccucaa ucugaagaca     2400

uaugaugaau cuuucaucaa agagcugaag ccuauuuuug uuagccggau gcccaagaga     2460

gcuaugaaag gacagaucca cgaagagacc cucaagcgcg ugcggggcag aaccgacgac     2520

ggguacaucc ggacagucaa aaaggugaaa cuggaucaaa uuccuuucga caagaacggc     2580

gauuuuccca uguucggaaa ggagaccgac augaaaacau augaagccau caagucccgc     2640

uaccuugagu cuggcaaaga uaagaaaaag gcuuuuaaua cgccgcugua caagccugcc     2700

aaaaaccccg aaaaugcgcc uauuaucaga agcauuaaga ucgaggaaaa agccaaccgg     2760

gugguuaugc ucgacgagaa gaccgcugcc gauaacgcuu ccaucguccg cacagaggug     2820

uucagacaca aagaaaccgg gaauuauuuc cugacacccg uguacgucgc cgacgugcug     2880

gccaagaagg uuccugaucg gcuuauuacc acgaaaaagc ccuauagcga guggagaaaa     2940

aucaccgacg aauacgaguu ucucuucucu cuguauccga acgaucugau ucgcgugaag     3000

augccuaaag agaaggaaac aaagacccau acaggagaga aacacgucug gcaggaaaau     3060

uuuauguacu ucaagggcgu gcauuccggg agcgcuggag uucggaugau cgaccacaug     3120

aacucuuuug aggaugaaau uggcgugaga aaacucgucg uguucgagaa guaucagguu     3180

gacccccugg ggcgcaugaa uaaagugcac ggcgagaagc ggcauggaau cuga           3234


<210>  3852
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3852
augaaguaca gcaucggccu ggacauuggg aucgccuccg ugggaugggc ugucauuaac       60

gaggauaucg aaagaauuga ggaccucggc guucggaucu ucgauguggc cgaacagccc      120

aaaaccgggg cgucucugag cacaccucgc agacuugcuc gguccacgcg caagagacug      180

cggcgcagac ggcaccgcgu ggagagaguc cggaaauuua ugauccaaaa gggacuccug      240

acccaggugc agauggaaca gcuuuaugac uggaaagaug gcgaccugga uauuuggcuc      300

guucgcguga augcccugga gagaaagcug ucugaccggg aguucgcccg cauccucguc      360

cauuuugcua agaacagagg guuccaaagc aaucggaaau ccgaacugca ggaggaagau      420

aacggagccg ugcuuucugc uguucaggag aauaagagac ugauggagga aaaaggcuac      480

cagacaauua gcgagaugcu ccuguaugac gaaaaguuug agggcaccaa acgcaacaaa      540

gggggagaau acacacacgu ggucgcccgg uccgaucuua agagagagau ccaacugauu      600

uucgagaagc agcgcgcgua uggccaugcc uuugcuacca uggaaaauga ggacaaguuc      660

cuccagaucu ggagcucuca gcggccguuu gccacaaaag augacauugc uaagaaaauc      720

gggaacugca ccuucgaacc caaggagaaa agagccccua aguccacgua caccuuugaa      780

cgcuuccggg cgcuggacaa gcugaauaga cuccgcaucc ugagcggaac aucucccgcc      840

cggucccuua cccaagagga gagagaucug auucucaaca gccuguuuuc uaaaaaggaa      900

gugaaauaua aggagcuucg caaacugcuc aagcugaaug acgaacagcg guucaacgag      960

aucuucuacg auccuaauga aaagauugag aaaaacgaga auagaacauu ucugucccuc     1020

gaaagccagu acaagauccg gaaaauucug caaaagaucg agggcaaauc ccuuaacgac     1080

accuaucgcc ccguggaucu ggacacgauc gcuuacgccc ucacagucuu caaggaugac     1140

caggauauua gagacuaucu gcagaaccag uacguugcuu cuaaugggaa gaaagaacuu     1200

aaccuggcca aucggaagua uaacgaugag cucaucgagg aacugcugca ccucagcuuu     1260

accaaauucg gacaucuguc ccuuaaggcc cugagaaaua uucucccuuu uauggagcaa     1320

ggcaucccgu accaccaggc uugugaaaaa gcgggcuauc aguucaacaa gaaggugaau     1380

cgcgagaaac agaagcugcu gcccccugug cccgccaaag acauugucaa cccugugguu     1440

auccggagcc uuucucaagu gagaaagguc aucaauggga uuaucaaacg cuacggaucc     1500

cccagcucua uuuacaucga acucgcuaga gagaugggcc ggggguauaa ggagcgcaaa     1560

gaccuggaaa agcaguacaa caagaauaga cagaugaacg agggagccaa aaagcacauu     1620

cuggaacucc ggccggaguu uggcgauccu cgcgggcaug acauccugaa auauaaacuu     1680

uggcaggaac aagauugcag augugccuac agcuucaagu ccauucccau caagcagcug     1740

uuugagaucg gauaugcuga gguggaccac auuaucccuu acucucggag cuucgaugac     1800

uccaauaaca auaagguucu cgugcuggcc gcggaaaacc agaacaaaca gaaucgcaca     1860

cccuaugagu gguuuggcca ugaugaaacc aaguggcaag aguucaccag ccuggucaca     1920

acgaugaaag ugucuaagaa aaagaagucc cuucuccuga aaaagaacuu ugacgaagaa     1980

caggcugagg aguucagaag ccggcaccug aaugauaccc gcuacauuac aagauacauc     2040

aaaaccuuca uugaggacaa ccuccaguuu cggauggaag agggcaagaa acaauauguc     2100

cacacaguga auggggccua caccucucug augagaaagc gcuggggauu caacaagaau     2160

cggcaggaga acgaucuuca ucacgcccug gacgcuguua ucguggccgu cucccucccc     2220

uuuaaacaua aggugagcgc uuauuucaaa agacgcgaag aguacgccca caaucagcug     2280

aagcgggaag gcgaguuuuu cccugaaccg uggcccgggu uuauccauga gcuggagauu     2340

agaaugaugc aggauuccga aaacaucaaa cuugcgaugg agucucucaa ucugaagacg     2400

uaugacgaga gcuucauuaa agaacugaag ccuaucuuug uuucccgcau gcccaagcgg     2460

gccaugagag gacaaauuca cgaagagaca cucaaacgcg ugcggggcag aaccgacgau     2520

ggguacaucc ggaccgucaa gaaagugaag cuggaccaga ucccuuucga uaaaaacggc     2580

gacuuuccca uguucggaaa ggagacagau augaagaccu augaagcuau uaaaucucgc     2640

uaccuugaga gcggcaagga caaaaagaaa gccuuuaaua caccgcugua caagccugcu     2700

aagaaccccg aaaaugcccc uaucauuaga uccaucaaaa uugaggaaaa ggcgaaccgg     2760

gugguuaugc ucgaugagaa aacggccgcu gacaacgcca gcaucguccg caccgaggug     2820

uucagacaca aggaaacagg gaauuauuuc cugacccccg uguacgucgc ugaugugcug     2880

gccaaaaagg uuccugaccg gcuuaucaca accaagaaac ccuauucuga guggagaaag     2940

auuacggaug aauacgaguu ucucuucucc cuguauccga acgaccugau ccgcgugaaa     3000

augccuaagg agaaaggaac caagacacau accggcgaaa agcacgucug gcaggagaau     3060

uuuauguacu ucaaaggggu gcauagcgga ucugccggcg uucggaugau ugaucacaug     3120

aacuccuuug aagacgagau cggggugaga aagcucgucg uguucgaaaa auaucagguu     3180

gacccccugg gccgcaugaa uaaggugcac ggagagaaac ggcauggcau uuga           3234


<210>  3853
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3853
augcagcaaa agccccagag auacauccug ggccucgacc uggggauugc cagcguggga       60

ugggcuugcg ucugggccga ugagaacgaa cacccuaccg gccuucugga cugugggguu      120

cggacauucg agcgcgcgga agugccgaaa acgggagauu cccucgcucu ggccagacgg      180

caggcccgcu cugugagacg gcuuauccgc agacgggcuc aucgccugcu cagacugcgg      240

cgccugcuca agagagaggg cguccugcuu agcgccgacu uuaaugaaaa cgggcuggug      300

cggggacucc ccauugaugc uugggcccug cgcguugcgg gccuugacag aaaacugucc      360

aauaaggagu gggccgcugu gcuccugcac cuggucaaac aucggggcua ucucucucag      420

agaaagaacg agacccaaag caaggauaaa gaacuggggc gccuucuggc cggagugaau      480

ggcaaccggg aggcucucca gaauagaucc gacgaauacc gcacagccgc ggagcuggcc      540

guuaagaaau ucgcugccga gaacaauggg ucucuucgga acaagggagg cgauuauagc      600

cacaccuuug acagaaaaga ucugcagcgc gaacuccauc ugcuguucga gcggcagaga      660

gcucucgaca auccuuuuac auccagcgaa auggaggccg ccguggauga acugcuuaug      720

aagcaacgcu cugcucugca gggggaggcg guccucaaga ugcugggacg gugcaccuuc      780

gagcccuccg aauacaaagc cgcuaagaac acauauagcg ccgagagauu uguguggcuu      840

accaaacuga auaaccuccg cauccaguac aauggcgaag aacgggcccu gucugaagcu      900

gagagacggc ugcuccugga cgagccuuau cagaaggcca aacuuacgua caagcaagug      960

cgcaccauuc uggaacuccc cgaguccgcg gucuucaaag gccugagaua cgggcacgga     1020

gaagacggcc ugaaggcuga gagcgggaca cuuauggaaa ugaaggccua ucacgccguu     1080

cggaaagcuc ucaaggaugc cggacuggag ggcgaguggg aaaacagaau ccgcaacccu     1140

gagcugcucg acgcuaucgg gaccgccuuu ucucuguaca aaacagauga agacauucgg     1200

gcgaaucuug ccgcuuucag acuguccgag aacgugcucg aggcccugcu ggcuggacuu     1260

aauuuugaua aguucgugca ucucagccug aaagcccugg cgaagauccu cccgcugaug     1320

gaacagggca ugcgcuauga cgaggccugu aaggaggcuu acggcgauca cuauggggcc     1380

aaagagcagg aagaccauag acuucugccc ccuuuugucg cugaugacau ucggaacccc     1440

gugguuuucc gcacccucac gcaagccaga aagaucguga augagauugu ccggcgcuac     1500

ggauccccuc ugagagugca caucgagaca ggccgggaag uugggaaauc uuucagcgau     1560

cgcaaggaga uugagaaaag acaggaagaa aaccggaagg agcgcgagag agccgcugcg     1620

cacuuuaagg aacuguuccc caauuuugcc ggagagccga aagcucagga cauccuuaag     1680

cuccggcugu augaacagca acauggcaaa ugccuguacu ccgggaagga gaucgaccuc     1740

agacgccugc cugaaaaagg cuacguggag auugaucacg cccuucccuu cagccggacc     1800

ugggacgauu cuuuuaacaa uaagguccug gugcucggaa aggagaacca ggacaaaaga     1860

aaucgcacac cuuaugaaua ccuggauggc aaggggaacu ccggagccug gcgggcuuuc     1920

gccgagagag ucagcggcug ucgcuuuccc uaugcgaaaa agcagcggcu gcuuacccag     1980

aaacucgacg aaaagaccga gcaaggguuc cuggagagaa aucugaacga uacacgcuac     2040

guggcucggu uucucugcca guucaucgaa gagaaaaugc aucuggaagg agccggcaag     2100

aagagaguuu uugccucuaa ugggcagauu acggcccuuc ugcggggcuu cuggggacuc     2160

aaaaaggugc gcgaggaaaa cgacagacac caugcucugg augcggucgu gguugcuugu     2220

agcuccgugu cuaugcagca gaaaaucacc cggcacuuuc gcuauagaga ggagcuggaa     2280

cacauugacc gggagacagg cgaagucauc cauggguucc cccaaccuug ggaguucuuu     2340

agacaggaag ugaugacccg cguguucagc gaugacccgg uuuccgagcu ugccgagaag     2400

cuggauagcc ggcccgaagc cguccacgag uuugugacac cucucuucgu gucuagagcu     2460

ccccaucgca aagucaccgg acagggccac cuggaaacga uccgguccgc caagagacuc     2520

gacgagaaga uuagcguggu uaaacugccu cuuacacaac ugaagaccaa agacaucgaa     2580

cgcauugugg gguaccccaa ccgggagccg gcucucuaug augcccuggc ggccagacug     2640

gcugccuuua aggacgaucc ugcuaaagcc uucucugagc ccuuuuacaa gaccggaaag     2700

gacggcaaau gcgggguccu ugugaaggcc guucgcgugg aaaaugucca gaaauccggc     2760

cugcucguga gaaaggauga gagcggaaaa augcagggcg uugcugacaa cgcgacaaug     2820

gugcgggucg auguguucgg gaagaaugga aagaacuacc uggugccugu cuaugccugg     2880

caaguugaaa aaggcgagcu ccccaaucgc gcuguggugg ccuacgccga cgaggaagau     2940

ugggacgaga uggaugacuc cuuugaguuc aaguuuucuc uguauccuaa cgaucuuguc     3000

gaagugguua ccaaaaagga caaauucuuu ggguacuaua gcggauucaa uagagcuaca     3060

ggcgccauca acauuaagga acacgaccug uccaaaucua aggugaagaa uggggucuac     3120

gagggcaucg gagugaaaac ggcgcagagc uuccagaagu aucagguuga uacccucggc     3180

aaaacaaucc ggcuguguaa gcccgagaaa cgcauggggu uuaagaagaa auga           3234


<210>  3854
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3854
augagauacg ugcugggccu cgacaucggg auugccagcu gcggaugggc ugucaucaac       60

caggagaagg aacggauuga ggaucugggc guucgcaucu ucgacaaagc cgaaaauccc      120

aaggauggga aaucccuugc ggcuccuaga cgggacgccc gcucuaccag acggacacug      180

cgcagaaaga aacaccggau gcaacgcauu aaggugcucc uggugaagca uggacuucug      240

agcaaaacgg agcucgauca ccuguaugaa uccgccaccg aggucgacgu guggaaccug      300

agacucgaug cucuggagcg gaaacuuuac ccgaaggaau uugcccgcau ccugauccau      360

cucgcuaaga gacggggcuu caaaucuaau cgcaaggaga caacccugag cgaaaacggg      420

cagauucuug aguccaucuc ugagaaucag cagauuaugg aaaagcaaaa cuauagaaca      480

guuggagaga ugauccugaa agacaagaaa uuugaaaauc acaagcggaa caaaaauggc      540

accuacgugg gcacagucac cagacagcag cuccaggaug agauucaaau gaucuuccau      600

gcccagcgcc uguauaagaa cgacuacgcg acggaagagu uugagagcuc cuaucuggaa      660

auuugggcca gccagcggcc cuacgcuucu aaagaucaga ucgagaagau gaucggguac      720

uguacccucg aaccuaagga gaaaagagcc cccaaggcuu ccuggagcuu ccaauauuuu      780

guggcccugc agacaauuaa uaaccuucgc cugaucucua aagaccggau ugaggagcuc      840

uccuucgagg aaaagaaaca gaucaugaau cuggcgcuug aaaagagcau uguuaaguac      900

aucgauauca gaaaacugcu cucugugccu aacgaauuuc aauucaauuc ccugagcuau      960

uccgccgaca ccgucgacac agcuguggag ucucgcaagu gcauugaacu gaaagaguac     1020

cacagcauca acaaggugua uaaacagauu uacggagaga acgcccucaa ucugcuuccc     1080

aaguauuacg auaccaucgc uuacggccug acgauuuuua aggacgauaa agacauccuc     1140

gaauaucugc agaacaagua cgucaaugcc aaagggaagc cuaucuccaa cuucgccaau     1200

aaaacauaug augacaccuu uauucaggcg cuucugaacc ucaauuucac aaagauggga     1260

caccugagcu ucaaggcucu gaaaaacauc auuccguuuc ucgaggaagg ccugucuuac     1320

gauaaggccu gugagaaagc uggguauaau uucaagggaa ccuccuacac cgagcaaaca     1380

aaauaucuuc ccguuauccc ucagaacacg aaucccgugg ugcaucgggc ccugagccag     1440

accagaaagg ucauuaacgc caucauuaaa aaguacggcu cuccuaaugc uauccacauc     1500

gaaacagccc gcgagcucag caagaccuuu caggaacgga aagagauuga cuccauguac     1560

caagauaacu cuaagaaaaa ugaacaugcg auccacaagc ugaaagagcu gggcaacauu     1620

aaccccagcg ggaucaauau ugugaaguuc aaacuuugga acgagcagga cggaagaugc     1680

auguauuccg gcaaguacau cgaaccgcac cgccucuuug aggaagggua uacagagguu     1740

gaucauaucc ugccuuacag ccggucucug gacgacuccu auaauaacaa gacccucacg     1800

cugggaacag aaaaucagag aaaaggcaac aagauucccu acgaguauau ggggaauacc     1860

agcaucugga acgauuucga gacccgggug cagucuaaua aaaagauuaa caagaaaaag     1920

caacagaaac uucugcucca gcacuuuucc uacgcucgcg aacaggaguu caucgaaaga     1980

aaucugaacg acacacggua cgccaccauu uaucugagcu cccuuaucca acagcaucuc     2040

aucuuuucug agagcuccag aaagaaaaag guccacacag ugucuggaau uaucacgagc     2100

caucugcgcu cccggugggg cuucaauaag gauagaaaag aaggccacau ucaccaugcc     2160

cuggacgcug uuaucguggc cgucaccagc gaucacauga uucagcgcgu gacaaaguac     2220

uauaaacuca aggagcugaa cagaaaucuu caagcuaaac ggaugccuuu ucccgagccc     2280

ugggaagggu uccgccugga gcucgaagcc agaaucucuc cuaacaccca gcaguaccug     2340

aagggacugc gguuuaaaaa cuaugcggac gucaaucuuu ccgagaucaa gagcauuuuc     2400

gugucucgca ugccgaagca uuccaucaca ggcgagcucc accaggaaac cauuagaaaa     2460

uucaucgggu acaacgagaa gggaaaaguu cugacggcca uuaagaccaa acuggaagau     2520

auccccuuug acgcuaaugg cgauuucccu auguauggga aggagagcga ccucuacaca     2580

uacaacgcca uuaaggaacg guaucugucu cacaaaaagg auaaacgcaa gagcuuucaa     2640

gagccccuuu acaaaccuac caaguccggc ggaaucggcc cccugaucaa gagcauuaaa     2700

aucauggaca caagaaccau ugugaauccg gucaaccagg ggaagggagu gguuuauaau     2760

ucuaaaaucg cucggacgga uguguucaag aaagaggaaa aguacuaucu cauuccuauc     2820

uacacaaucg accugaugaa gaacauucug ccccagaaag ccaucaccgc gggcaagggg     2880

uaugaggacu ggacaaccau ugauccuucc uuuaccuucc uuuuuagccu cuuccccaau     2940

gaccugaucc agauuguccc uuccaaaaac aagacaauca aagcccgcac gaccgugucu     3000

aagaaggaag ugcugcuccc cagccugaca ggauacuuua aaggcguuca uuccgggacc     3060

gcuggcauca cagucgagac ccacgaugga ucugugauug ccaauguggg cagcaagcaa     3120

cuucugcuca ucgaaaaaua ccaggucgac gugcugggga acuauacgaa gauuaaagag     3180

gagaagagaa ucggacugac aaccuacaau gauaacaaaa agauugcugc cuguuga        3237


<210>  3855
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3855
augaagcagc aaccccagag auacauccug ggccucgacc uggggauugc cagcguggga       60

ugggcucuua uccuggucga ugaggaaggc aacccuauug ggcuccugga cugcggaguu      120

cggaccuucg agcgcgccga agugccgaaa acaggcgauu cccuugcgcu ggcuagacgg      180

caggcccgcu cugugagacg gcucauccgc agacgggccc accgccugcu gagacuccgg      240

cgccugcuua agagagaggg gguccugagc gcugccgacu uuaauuccga uggacucccc      300

aaccugccua uugacacgug gcagcuucgg guggcuggcc uggaacgcaa acucaccaau      360

caagaguggg ccgcgguucu gcugcaucuc gugaagcaca gaggcuaucu gucucagcgg      420

aaaagcgaga cacagaccga aaacaaggag cuuggguguc ugcucgccuc cgucgcuaaa      480

aauucuaagc ugcuuagcga aaacgccaau caguacagaa cacccgcuga gcuggccgug      540

cgccucuucg cggagcaauc cggacaucug cggaacaagg gcggggauua uacccacaca      600

uuuaauagac uggaccucca gaaagaacug caucuucugu ucgagcagca gcgcaccuuu      660

ggaaacccuu ucgccagcgc ugaacuccaa ucuaagguug auggccugcu uaugaugcag      720

cgguccgccc ugcaggggga ggcugugcuc aaaaugcugg gaaagugcac guuugaaccc      780

agcgaguaca aggccgccaa acacaccuau ucugcugaga gauucaucug gcugacaaag      840

cucaauaacc ugcgcauccu ugaaaauggc accgagcggg cgcugaacga agccgagaga      900

aaacuccugc uggacgaacc uuacaagaaa gcuaagcuca cauauuccca agcccgcaaa      960

cuccuggaua ugagccacga cgccaccuuu aagggccugc gguacgggaa ggauucugag     1020

ggacucaaug cugagaacgg cgcccugaug gaaaugaaag cguaccauaa gauuagaacg     1080

guccuugaca aagcugggcu gaagggagag ugggaaaacc gcauccggaa uccggagauu     1140

cucgacaaaa ucggcacagc cuucucccug uauaagaccg augaagacau ucaggccgcu     1200

cuggccgauc uuagacuccc cgagaacgug cugaaugcuc ugcucgccgg gcugaacuuu     1260

gacaaguuca uccagcuuag ccugaaagcg cucuccaaua uuaugccucu gauggagcgg     1320

ggaauccgcu acgaugaagc cuguaaggag guguauggcg accacagagc ucuggccgaa     1380

ucuaaaaacc agaugcuucu ucccccuauc cccgcuaaug agauucggaa cccggucguu     1440

cugagaacac ugacccaaac ccgcaaggug aucaaugcca uuauccggag auacggcagc     1500

ccucagcgcg ugcauauuga gacagcgaga gaacucggga aauccuuuaa ggaucggaag     1560

gagaucgaaa aacgccagga ggaaaacaga aaggagcggg agcgcgccgc ugaacacuuc     1620

aaagagcugu ucccccacuu ugccggagaa ccuaagggcg gggacauccu uaaacugaga     1680

cucuaugagc agcaacaggg aaagugccug uacagcucua uucccaucga ucugggccgg     1740

cuuaaugaaa agggguaugu cgagauugac caugcucucc ccuucucccg cacgugggau     1800

gacagcuuua acaauaaagu gcugguucug ggcucugaga accagaagaa aagaaaucag     1860

accccuuacg aauaccucga cggagccagc aacucccaac gguggcgcga guuccaggcc     1920

agacugcagg gcugucgguu uucuuaugcu aagaaacaga gaauccaaac agcgaagcug     1980

gauagcaaaa ccgaacaggg guuccuggag cgcaaccuca augacacacg guacauugcc     2040

agauuucugu gcggauucau cgaagagaac cugcaccuuc agggcgaggg gaagcgccgg     2100

guguuugcuu ccaauggaca aaucaccgcc cugcucagag gccugugggg gcucaagaaa     2160

guccgcgaag agaacgaucg gcaucacgcc cuggacgcua uugugguugc cuguagcacg     2220

gugucuaugc agcagaagau cacaaaagcg augcagagac gcgaaacccu ugagaccguc     2280

gauacagaga ccggcgaagu gaagacacgg auuccgcaac ccugggacuu cuuuagacag     2340

gaggucauga ugcggguguu cuccgauacg ccucaugagg aacuggcuga aaaacucagc     2400

ggacgccccg aggcccugca cgacuuuguu ucuccucugu ucguguccag agcccccaau     2460

cggaagauga ccggccaggg gcaccuugag acagucaaga gcgcuaaacg ccuguccgaa     2520

aacaucucug ugguuagaaa gccgcucacc gcccugaaac ucaaggauau ugagaauauc     2580

gugggauauc cugaccggga acccgcgcug uaccaggcuc uuagagagcg ccugcaagcc     2640

uauaaagaug acccugucaa ggccuucgcu gaacccuuuu acaagccugc caaaaacggc     2700

gggcagggaa gccucgugaa ggcugugcgg guuaaagaug uccagaagac aggcgugaug     2760

gugagaaaag accgcggggg cauugcccgg ggaaucgcgg acaaugccac cauggucaga     2820

guggauguuu ucggcaagaa ggggaaaaac uaucuggugc ccgucuacgc uuggcaagug     2880

gccaagggaa uccugccgaa ucgcgcuguu guggccuacg ccgacgaggc ugauugggac     2940

gagauggaug acuccuauga auuuaaauuc acgcuuuacc cuaacgaucu ggucgagauu     3000

aucucuaaga aaggccggau uuuuggguau uacagcggac ucaauagagc gaccggcgcc     3060

aucgacauua aggaacauga ucuguccaaa gagaaggcua agaacgggau ccaccagagc     3120

gugggcguua aaacagugcu cucuuuccag aaguaucagg ucgacccccu gggaaaagaa     3180

aucugccuuu guaaggugga gaaacauccc gagcuuaauu ccaagaagaa aaaguga        3237


<210>  3856
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3856
augggcuacc ugaucgggcu cgacauugga aucaccagcg ugggcuaugc cguccugcag       60

acagauuuca aggggaaccc cuacaaaauu cuucugauga auacgguuau cuuuccugug      120

gcugaggccc cgaagaccgg agaaucccuc gcggcucccg ugagacaaaa acggucuagc      180

cgcagaacaa accggcgcac caaguucaga aaacugcggg uccagaaacu uuuugugcgc      240

uucggccugc ucgaguccgc cgacguugcc acauuucuga aucagaaggu gccucaccag      300

gauauuuggc aacugagaaa agacgcucuc aaccggaagc ugucuaaucg cgaacuuuuc      360

gagauccugu auuucuucgu cgggcauaga ggauuuaaaa gcaaccggcg cuccgaagug      420

accggcaagc agcccaaaga uaaucagguu gugcucacau cuauccacga cagaggcaac      480

acccugaaug auuucgacag ccuuggggag augaugcagg agcugccuga auuuaagacg      540

auuaagcaua acaaagccua ccggcccgau aauaccaucu auccucuccg cgcuuggauu      600

gagcacgaac uguccgccau cuucagcguc caacagaaga acaauagaca gauuacagac      660

aacuuuauca cccaguaccu ggauauuuuc cggucucaaa gagacuucga ucagggaccg      720

gcggcccccu cccgcuuugg cgggaauaug aucgagcgga uggugggacc ugacagccuc      780

gacgcucaug agaaaagagc cgcuaagcug acaaacaccu ucuuucgcuu caacuuucuu      840

ucuacgcuga auaaccucaa aauccagcug acacuuggcg ccgaauuuca cccccugacc      900

acaccucaac gggagauugu gcuccaggcg gccacccuga agucccugaa uuauaaacag      960

aucagacaca agcuccagcu gcccgauagc gcucgcuuua accuuguccc guaccaaucu     1020

aaugaagacc cuauuaagac cgaguccaaa cauuaccugu ucaagcggaa agcccucaca     1080

cagaucgaag cuguuacgga uaacccccag cugauugaca agaucgggac cauccuugcc     1140

uauuuuaaag gagauaagac aagacaggag aagcugagcg cccucaaucu gucccgcgcu     1200

gagauuaaac aacugcuccc uaucaaccag cccggcuucg gcaaucuguc ucuuaagacc     1260

augcacaaaa uucugcccua ccucgaacag gggcaucugu aucacgaggc ggcccagcug     1320

gcuggauacg acuuucaaaa caaucuugug gaucggcagu aucucagaga caaccugaag     1380

aauccugugg ucaaccgcgc caucggcaaa acaauuaagg ugguuaaugc caucaucaag     1440

aaauacgggg auccggacgc uauucacauc gaacugaccc gggagauuaa gcauagccac     1500

gaagagagac agaaaaucgc ccggcagaug aacacgaaca uggaucaaaa ugacaaggug     1560

gcggagcauc uccgcgaaaa cggcauuccc gucaauggac agaacaucau uaaauccaag     1620

cuguucuaug agcagaaggg gaucgaccuu uacagcggac aacagaucaa ugaacaggau     1680

cuguacacag aggaccacua ucagguggau cauauuaucc cuuacucuau uuccuuuaac     1740

gacagcuuca ccaauaaaac cguuacaaga accucuaaca aucaaaacaa ggcuaaucgg     1800

cucccccugg aauaucugag cgaggagaaa caggaugccu ucagagcccg cgugaugucc     1860

aacaucucua auuaccggaa gagacagaac cuucucaaag aaggcauuac agaggacaau     1920

cgccagcaau ggaagacgag aaacaucaac gauaccggcu auaucaaucg gcugcugagc     1980

caguaccucc gcaacaaugu ccaguuuaca aagaaguccc acaacccugu guucaccguc     2040

aauggggcua caaccgccua uauuagaaag cgguacggaa ucauuaaaaa ccgccaggcu     2100

acggaccugc accaugccgu ggaugcgguu aucauuggcu gcaucagcaa uaccuuugug     2160

ucuagacuug ccaaguacuc cgaagcucaa gaggcccggu aucacaaaca gcuguggcag     2220

aucacacaau accgccagga gcauaaggac cucagcacca uugaauauca ggagcugauc     2280

aaacaguucc cccugccgug gccugauuuu agacacgaac uuauggcucg gcucucuaca     2340

gaccccgcca agaugauggc gcaccauacc ugggagcacu acacggauga agagauuucc     2400

caacugaagc cugccuucgu cauccgcagc gcuaaccaua aagugacagg guccgcccac     2460

gacgacacca uuuauucuaa gaaacuguac gaucagagag gaguuggccu ccugcggaca     2520

agccuucaga aucugaagua ugacuccaaa aaggauggga ucaagaacua cgagagacuc     2580

aaagacggcu cuaauagccu gguguacgau ucccugcuuc gcgcucucaa ggaacaugac     2640

ggaaacgcca aaaaggccuu ucccgauaau cagcugcuga ucagcaccgg caaccaccaa     2700

cagauuauca cccacgucaa aacagugaag aagauuacgc auccuaucga ggugaaucgg     2760

gacaccgcua uugcgaucgg gggaagaaug auucgcguug auaucuucaa acaggccaag     2820

gcucagaaac ucaucggcau ucccgucuau gugaacgaca cagugaagcc gacccugccu     2880

aaccgggccu guacaucugg gaaaagauac uccgauuggu aucaacuuac ccgccaggac     2940

caguuucugu gcagccucgu cucuaaugau cuggugcugg uugaaacggc cagcccccuu     3000

aaggugacaa acaauaucac caacgaggaa guccagacca cacaacugcu cuguuacuuc     3060

uccucuuuua auauugcuaa cgcccagauc aauuuccaca gcauuaacaa ugacuccagc     3120

aucaacccca ucgugcugaa uaaaguuacc uaucucacaa aguaccagcu ggacuaucuu     3180

ggaaacuacu uuccugugaa gcaugagucu cggaaaacgu ucaauagaaa gcgcuga        3237


<210>  3857
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3857
augcacacca gacuggcccu cgacaucggc acaacgagca uuggguggau ccuguacgag       60

cuugugaacg gacagcccgc uuccauucug gauggcggga cccggaucuu cucugacgga      120

cgcgaagcca agacaggcca uagccucgcg gucgggagac ggaccgcucg cggaaugaga      180

cggcgcagag aucgguaucu gcgcagacgg gccgcccuua ugaaacgccu ggcugccauu      240

ggccucaugc cugagucccc ggaagcugcc gcgcaacugg ccacacugga ccccuacgag      300

cucagagcuc ggggccugga ucacccucuu cccccugaac aucuggggcg cgcccucuuu      360

caccugaauc agagacgggg auucaagucu aacagacgca ccgacaaagg caauaacgaa      420

gggggaauga ucgcugaugc cagcgcgcgg cuugagcagg ccaugauggc ugccggcgcu      480

agauccuaug gggaauuucu gcauaagcgc cgggccgagg ccacagcucc cagagcgacc      540

ccugccguuc gcacgcggau caauccgcac cucaccggag aagacggcaa agagcagccc      600

ggcuacgauu ucuauccuga cagaaagcac cuggaggaug aauuucaucg ccugugggag      660

gcucaagccc ggcacaaccc cgaccucaca cccgccaugg gggaucugcu uuuccagacc      720

auuuuucauc agagaccccu gaaggcuccg gccgugggac gcugccucuu ccuggacgaa      780

ccucggcuuc ccaaagcgca cccucugaca cagcaaagaa cccucuacga gacggugaau      840

augcugcgga ucgcuccucc cggccagccu aagcgcagac ugucucgggu ccagagagac      900

cagauugugc acgcccucaa caaucgcaaa auccuggcca gcccggggaa gaacguucuu      960

acacuggcug ccauuggaaa acuccugaaa cuucggcccg gcgaggcuuu uucccuggau     1020

accgccaaua gagacgcgau cgccugugau ccugugcgca ugagccucgc uuaugagggg     1080

agauucggac cccaaugguc ugcccugucc gucgagagcc agugggaacu gauucagcgg     1140

cuccgcgacg aucaagacgg cgcugcccug guggauugga ucauggacag auacggcuuu     1200

gaucgggacc gcgcgagagc caucgcugau gccccucuuc ccgagggguu cggacggcug     1260

ggcgaaacag cuacccgcgc cguucucgcc gugcugauug ggggaaccac agagcaggaa     1320

guccugacgu acucugaggc uguggcggcc ugcggcuggg accauuccag acuugggacc     1380

ggcgaggugc ucgaccggcu gccguauuac ggacaggucc uggaucgcca cguucucccu     1440

ggcacaggga gacccgaaga cgaugagauc acccgguuug gaagaauuac aaacccuacc     1500

gugcauaucg gccugggaca ggugaagcgc cuugucaaua aggugauuga cgcuugggga     1560

cggccccacg aaaugguugu ggagcuggcc agagaucuca agaugagcga agaccaaaaa     1620

cgcaaccugg ccaaggucaa ugcucagaac cagcgggccg cggagaugag aucugcuaaa     1680

cugaucggcg aucuuaagau cgaggacaac gggcgcaauc ggggcauucu cagagugugg     1740

gaagaguccg ccgccgaugu ucugcaucgc uguugcccuu auacgggagc ucagaucagc     1800

gccuccaugc uguucgacgg cucuugugau auugaccaca uccuccccua cagccggaca     1860

cuggaugacg gguucaacaa uagagugauu ugccuuaaau ccgcgaaccg ggaaaagcgc     1920

aaucugaccc cgugggaggc uuggggaaac accccuaagu gggccgccau cgaggcuaau     1980

cucaaaaacc ugccucccgc caaggcuugg agauuugccc ccgacgcgau gcugcgguau     2040

gaaggcgagc gcgauuucac agccagagcu cuugucgaca cccaauaccu cagccggcug     2100

gccagagaau aucuggagug ucucuacgcu gaagauaucg acccggggcc ccacucuggg     2160

aaaaagccuc gccggcccau uugggugguc acaggcaaac ugacggagcu ucugagacgc     2220

cauugggggc ucaauucccu gcugccugau gcccaccggg gcaccgccaa ggcuaagaac     2280

agacuugacc aucgccacca caugaucgau gcggccgugg uuggagcuac agaccaugcc     2340

accgugcagc ggauugccaa uauggcuaaa ggcaacgucg agaaugggcu cgaagaucug     2400

gccggaacaa uccugccccc uuggcccggc cucagagacg aucugaaggc gcgccuugag     2460

agaaccauug ugagccaccg ggggaaccau ggagcuaucg acccggccgc ccgcaaacag     2520

ggcagagaua cgaccucugg gcagcugcac aaugacacag cuuacggccu caccggagaa     2580

acacugaagg gcguuccucu gguggucaac cggaaaccca uugacaugcu uaccccugcc     2640

augaucgaua agauccgcga cccccaucug agcagauggc ucacggcggu gacaaagggg     2700

aaagagggaa aggauuuuaa agcugccgug gaagccuucc gggcucaccc gggcccuuau     2760

agagggauuc gccggguuag acugaucgag cccgucgagg ugauucgcau caaggaccgg     2820

aacggaagac cuuacaaagg cuauaagggc gauuccaauc aaugcuacga aguguggugu     2880

cuccccgacc ggacccugac acaccauguc gugucuaccu uugccgcuca ccaggggcag     2940

accggaaagc cccauccugc cgcgaaacgc cuugccagac uguauaaggg cgauaugguu     3000

cggcucgacc gcagcaaauu cggguccgug auugcuacag ucgagaaguu ugauagaaac     3060

ggacagcugg aacuggugcc gcacaaugag ggcaacgccg accaacggua ccgcaaagau     3120

aagagcgacu cuguuuucau cagacuucgg gcugccacgu uuaucgccuc cggggcucgc     3180

agagugauug ucgacgaaau gggacggauc agagaucccg gcccuggcaa acauuga        3237


<210>  3858
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3858
augaaguacg gcaucgggcu ggacugcgga auuaacagcg ugggcuauug uaucauggag       60

cucaauuccg augacgaacc caaaagaauu guccggcuga acucucgcau cuucaccgag      120

gccgaaaauc cuaaggaugg gagcucccuu gcugccccga gacgggaggc gcgcggacug      180

agacggcgca uuagacggca caaacaucgc cucgaaagaa uccgggcucu gcuuguucgc      240

gagaacaucg ugacacaggc cgagcuggac aauauguuuu cuggcacgcu cagcgauauu      300

uacgaacuga gaaccgccgc ucuggacgcu ccccucaaca auaucgaguu cgcccgggug      360

cugauuaacc uugcccaacg cagaggguuu aaguccaauc ggaaaucuga aaagagcucc      420

agcgauaagg agacaggaaa acugcucucu gcgaucaaga aaaacaagga guccauugaa      480

gccggcggcu auagaaccgu cggggagaug cuguucaaag acgaacgcua cgcuaagcac      540

aagcggaaua aaggagaggc cuaucuuaac acagugagca gagauaugau cgaagcugag      600

auucaugccg uuuuugcguc ucagcgcaau uucggcaugc cuuuugccaa cgagaagauc      660

gaaaaugagu acaccgacau cgcucugucc cagcggcccu ucgaucucgg gccuggagaa      720

ggccccgaga acugcaaaag cccuuaugcc gggaagcaga uuaaaaacau gaucggacaa      780

uguacauuuu ucccggaaga gaagagagcu gugaaagccu cuuacuccuu ucagcuguuc      840

agccugcucc agggcauuaa uaacaucacc cuggucuccu cugacggcaa uacguuuccc      900

cuuagccagg agcaacgcaa gacccugaag gauuacugcu ucaaaacagc caacgugacc      960

uaugcuucca uucggaaggu ucuccagauc ccucaggaau acacauucaa aaauaucacc     1020

uauacggacg acgggauuga aaagagcgaa aagaagacaa aguuucagca ccugaaaccc     1080

uaccaugaga ugaagaaagu gcuuggaucu gcgauccaaa cccugacacc ugaggagcuc     1140

gaugagauug gcgaaaucuu caccuuuuau aaggacgaga ccauuaucau caaacagcug     1200

gaagagacag agauugauaa gucccuguac gaccuccugc uucagcugcc cacguucgaa     1260

aaggccgggc acaucagcac caaggcuugu aagaugauua ucccguuucu cgaggaagga     1320

aaaacauaug auaaggccug cgagugugcc ggccugaacu ucaaagcuca caccaauaug     1380

caaaagcaga ugcuucugcc ugccaagucu gacgaacuca acgaugucgu gaaucccgug     1440

gucagacgcg cgauuagcca gacaaucaaa guugugaacg cuauuauccg ggagaugggg     1500

accuccccua cguacaucaa uauugagcug gccagagaac ugucuaagag caaaaaggag     1560

cgcgacgaaa ucaacaaaaa guacaaacuc aaucaggccg agaacgaacg gcugaagaau     1620

gagauuaucg auaacuuuaa gauuaaaccc aacggacaag acaucaucaa acuuaaacug     1680

uggcaugagc aggaugacgg cauuugcccc uauacaggcg ugagaaucaa guacgaaaau     1740

cucuucgacg auggguaugc ugagauugac cacaucauuc cuuacucccu gagcuuugau     1800

gacucuuuca acaauaaaau ccugaccacc gccacacaga accggcagaa gggaaaucgc     1860

aucccgcuug aauaucuccc cgaguccgaa aaggauggcu uuagagucug ggugaacagc     1920

aauauucgga accauagaaa acgccaaaau cugcugaagg agcacuucac cgaggcugac     1980

aaaaaggaau uuaaaucucg gaaccucaau gauacaagau accugaacaa acuucugcuc     2040

aauuauauca acgacaaucu gcuguucacg gauuucuaca acgggaagaa aaagcacguu     2100

accuccguga acggagccgu cacagcguac auucgcaaaa gauggggcau caauaagaac     2160

cgggaggacg gggaucuuca ucacgccgug gacgcugccg uuauugcuug ugugacccag     2220

ggcaucauca aucgcauuac agccuauagc gcgagaaacg aagccccuua cgaucucaau     2280

accgagacgg gagagaucaa agaacgguuu ccccugccuu aucccaacuu caagaaggag     2340

cuggacgcuc gcaccacaau ugaagacgag aaaagacuca agcggguccu gauguccuuu     2400

ucuaauuaca gcuaugauga cguggaaaaa gccaagccgu gcuucgucuc ccgcaccccu     2460

cauagaaaag ugacaggcgc ugcccacgag gagaccaucc ggucugggaa gguugaagga     2520

uuuacgguga gcaaaacaga gcuuaccaag cugaagcuca caaacggcga aauugagggg     2580

uacuaugaac ccgaguccga uacccugcug uacaaugccc uucuccagcg ccugaacgag     2640

uucggaggcg acgcuaaagc ggccuuugcu gaugaguucc auaagccuaa agccgacggg     2700

acccccggcc cugucgugaa gaaaguuaag acaaucaaga gaucuagcuc caaugugaaa     2760

gcccggaaca agaauggacu gggcgucgcu gauaacggga gcaugauuag aaucgacgug     2820

uuuuacgugg aaaaugaagg auauuacuuc guucccaucu augucgccga uacggugaaa     2880

gagaugccga acgaagcgug uccuuuuggc aaggacaaaa agaagauuau gaccgaugac     2940

gauuuccucu uuucucugua ccccaacgac cuuaucaaaa uuacagcuaa gaaagacaug     3000

aaguucuccc uggccaauaa agagagcggg cuccccaaga acaaguaugc caaugaggug     3060

cuggucuacu auucugcugc cgauaucagc aacgcgucca uuagcgugau caugcacgac     3120

aaugcuuaca aauuccgcgg augcggcauc aagucucugg uuaaacuuga aaaguacacc     3180

guggauccuc ucgggaacgu cuccaaagug aaucgggaga agagaaugua uuuuaaguga     3240


<210>  3859
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3859
augagacugg gccucgacau cgggaccagc uccauuggau gguggcugua cgagacagau       60

ggcgccgggu cugacgcucg gaucacggga guggucgaug gcgggguucg cauuuucagc      120

gacggaagag aucccaaguc cggcgccucu cuugcggugg accggcgcgc ugccagagcc      180

augcggcgca gacgggaucg cuaucugaga cggcgcgcua cccucaugaa agugcuggcc      240

gaaacaggcc uuaugccugc ugacccggcc gaggcgaagg cccuggaagc ucucgauccc      300

uuugcccuga gagcugccgg gcuggacgag ccucuccccc ugccucaccu uggacgggcg      360

cuguuccauc ucaaccagcg cagaggcuuu aaaagcaauc ggaagaccga uagaggggac      420

aacgaauccg gaaaaaucaa ggaugccaca gcucgccugg acauggagau gauggccaau      480

ggcgcucgga ccuacgggga guuccuucac aagagacgcc aaaaagccac agacccccgg      540

caugucccuu cugugagaac ccgccugagc auugccaacc ggggaggccc ggauggcaag      600

gaagaggcug gguaugacuu uuaccccgau agacgccacc ucgaagagga guuccauaaa      660

cugugggcgg cccagggagc ucaccacccu gaacugacgg agacccuccg ggaccugcuu      720

uuugaaaaga ucuucuuuca gagaccccug aaagagccug aaguuggccu cugccuguuc      780

uccgggcauc acggagugcc cccgaaggau ccucgccuuc ccaaggccca uccucugaca      840

cagcggagag uccucuauga gaccgugaau caacugcggg uuacagccga cggccgcgag      900

gcuagacccc ugacccggga agagagagau caggugaucc acgcccucga caacaaaaag      960

cccacgaaaa gccugucuuc caugguccuu aagcugccug cgcucgcuaa agugcugaaa     1020

cuucgcgaug gggaacgguu uacacuggag accggaguga gagacgccau ugccugugau     1080

ccgcuccgcg cuagccccgc ccacccugac agauucggcc cccggugguc uauccuggac     1140

gcugaugccc agugggaagu cauuucccgc aucagacggg uucagagcga cgcggagcau     1200

gccgcucugg uggauuggcu cacagaggcc cacggccugg accgcgcuca ugccgaagcg     1260

accgcccacg cuccucuucc cgauggguac ggaagacugg gccucaccgc cacaacgcgg     1320

auucuguauc aacugaccgc ugacgugguc acauacgccg augccgugaa ggcuugcggg     1380

uggcaucacu cugacggacg caccggcgag uguuuugaua gacuuccgua cuauggggaa     1440

guucucgagc ggcacgugau cccuggcucc uaccaucccg acgaugacga uauuacacgc     1500

uucggaagaa ucaccaaucc uacgguccac aucggccuga accagcugcg gagacucgug     1560

aaucgcauua ucgaaacaca ugggaagccc caccagauug uuguggagcu ggcgcgggac     1620

cuuaagaaaa gcgaggaaca gaagagagcc gacaucaaac gcauucggga uaccaccgag     1680

gcugccaaga aaagauccga aaagcuggag gagcucgaaa ucgaggacaa cggacgcaau     1740

cggaugcugc ugagacuuug ggaagaucuc aacccugacg augccaugcg ccgguucugc     1800

cccuauacag gcaccagaau cucugcugcc augauuuuug acgggagcug ugaugucgac     1860

cacauccugc cguacucccg gacacuggau gacagcuucc cuaaucgcac gcucugccug     1920

agagaggcga accggcaaaa gcgcaaucag acccccuggc aggcuugggg agauacaccc     1980

cauuggcacg ccauugccgc uaaccuuaaa aaccugccug aaaauaagag auggcgguuu     2040

gccccggacg cgaugaccag auucgagggc gagaacgggu uucucgaucg cgcucugaaa     2100

gacacacaau aucuggcccg gaucucuaga uccuaccuug acacccucuu cacgaagggc     2160

ggacaugugu gggucgugcc cggccgcuuu accgaaaugc ugcggagaca cugggggcug     2220

aauagccucc ugucugaugc cggacgcggc gcuguuaaag ccaagaaccg gacagaccau     2280

agacaccacg cuauugaugc cgcggugauc gccgcuaccg acccugggcu ucugaaucgc     2340

aucagcagag ccgcuggaca gggcgaggcc gccgggcagu ccgcugaacu cauugcgcgg     2400

gauacacccc cucccuggga gggcuuccgc gacgaucuga gaguccggcu ggaccgcauc     2460

auugugucuc auagagccga ucacggacgg aucgaccaug cugccagaaa gcagggccgc     2520

gauagcaccg ccgggcaacu ucaccaggaa acggcuuauu ccauuguuga cgacauccau     2580

guggccagcc ggacagaucu ccugucucug aaaccugcgc agcuccugga cgagcccgga     2640

agauccggcc agguccgcga uccgcaacuu cggaaggcuc ugagaguggc caccggggga     2700

aaaacaggca aggacuuuga gaacgcccuc cgguacuucg cuagcaaacc ugggcccuac     2760

caggccauuc gcagagugcg gaucaucaag ccucugcagg cgcaggcucg cguucccguc     2820

ccggcccaag auccuauuaa ggccuaucag ggcggaucua aucaccuguu ugaaaucugg     2880

agacuucccg acggcgagau ugaagcucag gugaucaccu ccuucgaggc ccacacccug     2940

gaaggggaga aacggccuca ucccgcugcc aagcgccucc ugagagugca caaaggagau     3000

auggucgcgc ucgagcggga cggccgcaga gugguugggc augugcagaa gauggauauu     3060

gccaacggac uguuuaucgu cccccacaau gaagcuaacg ccgacacacg gaauaacgau     3120

aaaagcgacc cuuucaagug gauccaaauu ggcgcuagac cggccaucgc cuccggcauu     3180

cgccgggugu cuguugauga gaucgggaga cuucgcgacg gaggcacgcg gcccauuuga     3240


<210>  3860
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3860
auggacgagg aagccaagau ccagcugagc aaagaguccu acgaagagga acucgagucu       60

cugcuugaaa agagcggcga agaguucaga gauguguuuc ugcaagcuaa aaaggucuau      120

gacgccauuc uccuguccga uauccuuucu accaaaaagc agaacagcaa ggcgaaacug      180

ucccucggga ugauugaacg guacgacucu cacaagaaag aucuggagga gcugaagcag      240

uucguuaaag cuaaucuccc cgaaaagaca gccaucuuuu ucaaggacag cuccaaaaac      300

ggauaugccg gcuacauuga ugggaagacg acccaggagg acuuuuauaa auuccugaag      360

aaagaacuua auggaaucgc uggcagcgag cgcuuuaugg aaaaggugga ucaagagaac      420

uuccugcuca aacagagaac aaccgccaau ggggugaucc cucaucaggu ccaccugaca      480

gagcuuaagg cuauuaucga acggcagaag ccguacuauc ccuuucugga ggaagcccgc      540

gacaaaauga uuagacuccu gaccuuccgg aucccuuacu acgugggacc ccuggcgcaa      600

ggcgaggaaa caucuuccuu ugccuggcuc gagcgcaaga ccccugagaa aguuacgccc      660

uggaacgcua ccgaagugau ugauuauagc gccucugcua ugaaguucau ccagagaaug      720

auuaauuacg acacauaucu gccuaccgag aaaguccuuc cgaagcauuc cauccuguac      780

cagaaguaua caaucuuuaa cgaacucacc aaaguggccu acaaggauga gcggggcauu      840

aaacaccagu ucagcucuaa ggaaaaacgc gagaucuuca aggagcuguu ucaaaagcag      900

agaaaaguua cggugaagaa acuucagcaa uuccuguccg cgaauuauaa cauugaagac      960

gccgagaucc ucggggucga caaggcuuuu aauagcuccu acgccacaua ccaugauuuc     1020

cuggaccugg cuaaacccaa caccgaacgg guggccgagc uccuggagca gccugaaaug     1080

aaugccaugu uugaggauau ugugaagauc cuuacaaucu ucgaagaccg cgagaugauu     1140

agaacccagc ugaagaaaua ucagucuguc cucggagaug gcuuuuucaa gaaacugguu     1200

aagaaacacu acaccgggug gggacggcuu agcgaacgcc ugaucaacgg cauuagagac     1260

aagaaaacaa auaagacgau ccucgauuau cugauugacg augacgauuu ucccuacaac     1320

cggaacagaa auuucaugca acugaucaac gacgacuccc ucagcuuuaa ggaggagcug     1380

gcuaaugaac uugcgcuggc cgggaaccag ucucuccugg agguggugga agcucugccu     1440

ggaucccccg ccaucaaaaa gggcauuugg cagacccuua aaaucgucga ggaacucauu     1500

gagaucauug gcuauaaucc gaagaacauc gugguugaga uggcccgcga aaaucagcgg     1560

acaaacagaa gcaaaccucg ccugaaggcu cuggaggaag cccucaaguc uuucgauagc     1620

ccccugcuua aagagcaacc uguggacaau caggcgcugc agaaggaucg gcucuaccug     1680

uauuaccugc agaacgggaa agacauguac accggagaag cucuugauau ugacagacuc     1740

uccgaguaug auaucgacca caucauuccc cgcucuuuua ucgucgauaa uagcauugac     1800

aacaaggugc ugguuuccag caaagagaau cggcugaaga uggaugacgu gcccgaucaa     1860

aaggucguga ucagaaugcg ccgguacugg gaaaaacucc ugagagccaa ccuuauuucu     1920

gagcgcaagu ucgccuaucu gacaaaacuc gaacugaccc cugaggacaa ggcucgguuc     1980

auccagagac agcuggucga gacgcggcag aucacaaaac auguggccgc uauucuugac     2040

caauacuuua aucagccgga agaguccaag aacaagggca uccgcauuau cacccucaaa     2100

agcucucugg uuucccaguu cagaaagacc uuugggauuc acaaagugcg ggaaaucaac     2160

aaucaucacc acgcccauga ugcguaucug aacggagucg uggccaucgc ucuccugaag     2220

aaauacccca aacuugagcc ugaguucguu uauggcaauu acacaaaguu uaaccuggcc     2280

accgagaaua aggcuacagc caagaaagag uucuacagca acauucucag auuuuucgaa     2340

aaggaggaau auuccuacga cgagaauggg gaaaucuuuu gggauaaagc gcgccacauu     2400

ccccaaauca agaaagugau uucuagccau caggucaaca ucgugaagaa gguggagguu     2460

cagacgggag gcuucuauaa agagaccguc aauccuaagg gcaaacccga caagcugauc     2520

cagcggaaag ccggguggga uguguccaag uacggaggcu uugggucucc gguggucgcu     2580

uaugccgugg cuuucauuua cgaaaaggga aaagccagaa agaaagccaa ggcuaucgag     2640

ggcauuacaa ucaugaaaca aagccuguuu gaacaggacc cuauuggguu ccuuuccaac     2700

aagggcuaua gcaauguuac caaguucauc aaacucucua aguacacacu guacgagcug     2760

gaaaacggac gcagacggau gguggcgucc cacaaagagg cccagaaggc uaauagcuuu     2820

auucuccccg agaaacuggu cacccuucug uucuaugccc agcauuacga ugaaaucgcc     2880

cacaaggagu cuuuugacua ugugaacgau caccucuccg aguuccgcga aauccuggac     2940

caaguuauug auuuuagcaa uagauacacg aucgcugcca agaacaccga gaaaauugcg     3000

gaacuguucg agcagaacca ggaaucuaca gugcagagcc uuucccagag cuuuaucaau     3060

cugaugcaac ucaacgcuau gggcgccccu gccgacuuca aguuuuucga ugucauuauc     3120

ccccggaaac gcuauccuuc ucugaccgag aucugggaau ccacaauuau cuaccagagc     3180

auuaccgggc ucagagagac gcggacacgc auggcuaccc ugugggacgg agagcaauga     3240


<210>  3861
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3861
auggacgagg aagccaagau ccagcugagc aaagaguccu acgaagagga acucgagucu       60

cugcuugaaa agagcggcga agaguucaga gauguguuuc ugcaagcuaa aaaggucuau      120

gacgccauuc uccuguccga uauccuuucu accaaaaagc agaacagcaa ggcgaaacug      180

ucccucggga ugauugaacg guacgacucu cacaagaaag aucuggagga gcugaagcag      240

uucguuaaag cuaaucuccc cgaaaagaca gccaucuuuu ucaaggacag cuccaaaaac      300

ggauaugccg gcuacauuga ugggaagacg acccaggagg acuuuuauaa auuccugaag      360

aaagaacuua auggaaucgc uggcagcgag cgcuuuaugg aaaaggugga ucaagagaac      420

uuccugcuca aacagagaac aaccgccaau ggggugaucc cucaucaggu ccaccugaca      480

gagcuuaagg cuauuaucga acggcagaag ccguacuauc ccucucugga ggaagcccgc      540

gacaaaauga uuagacuccu gaccuuucgg aucccuuacu acgugggacc ccuggcgcaa      600

ggcgaggaaa cauccagcuu cgccuggcuc gagcgcaaga ccccugagaa aguuacgccc      660

uggaacgcua ccgaagugau ugauuauucu gccuccgcua ugaaguuuau ccagagaaug      720

auuaauuacg acacauaucu gccuaccgag aaaguccuuc cgaagcauag cauccuguac      780

cagaaguaua caaucuucaa cgaacucacc aaaguggccu acaaggauga gcggggcauu      840

aaacaccagu uuucuuccaa ggaaaaacgc gagaucuuca aggagcuguu ccaaaagcag      900

agaaaaguua cggugaagaa acuucagcaa uuucugagcg cgaauuauaa cauugaagac      960

gccgagaucc ucggggucga caaggcuuuc aauuccucuu acgccacaua ccaugauuuu     1020

cuggaccugg cuaaacccaa caccgaacgg guggccgagc uccuggagca gccugaaaug     1080

aaugccaugu ucgaggauau ugugaagauc cuuacaaucu uugaagaccg cgagaugauu     1140

agaacccagc ugaagaaaua ucagagcguc cucggagaug gcuucuuuaa gaaacugguu     1200

aagaaacacu acaccgggug gggacggcuu uccgaacgcc ugaucaacgg cauuagagac     1260

aagaaaacaa auaagacgau ccucgauuau cugauugacg augacgauuu ccccuacaac     1320

cggaacagaa auuuuaugca acugaucaac gacgacagcc ucucuuucaa ggaggagcug     1380

gcuaaugaac uugcgcuggc cgggaaccag ucccuccugg agguggugga agcucugcuu     1440

ggaagcccug ccaucaaaaa gggcauuugg cagacccuca aaaucgucga ggaacugauu     1500

gagaucauug gcuauaaucc caagaacauc gugguugaga uggcccgcga aaaucagcgg     1560

acaaacagau cuaaaccgcg ccugaaggcu cucgaggaag cccuuaagag cuuugauucc     1620

ccucugcuga aagagcaacc cguggacaau caggcgcucc agaaggaucg gcuguaccug     1680

uauuaccuuc agaacgggaa agacauguac accggagaag cucucgauau ugacagacug     1740

ucugaguaug auaucgacca caucauuccu cgcagcuuca ucgucgauaa uuccauugac     1800

aacaaggugc ugguuagcuc uaaagagaau cggcucaaga uggaugacgu gcccgaucaa     1860

aaggucguga ucagaaugcg ccgguacugg gaaaaacugc uuagagccaa ccugauuucc     1920

gagcgcaagu uugccuaucu cacaaaacug gaacugaccc ccgaggacaa ggcucgguuc     1980

auccagagac agcuugucga gacgcggcag aucacaaaac auguggccgc uauucucgac     2040

caauacuuca aucagccuga agagagcaag aacaagggca uccgcauuau cacccugaaa     2100

ucuucccugg uuagccaguu uagaaagacc uucgggauua acaaagugcg ggaaaucaau     2160

aaccaccauc acgcccacga ugcguaucuc aauggagucg uggccaucgc ucugcuuaag     2220

aaauacccga agcuggagcc cgaauuuguu uauggcaacu acacaaaauu caaucucgcc     2280

accgagaaca aggcuacagc caagaaagag uuuuacucca auauucugag auucuuugaa     2340

aaggaggaau auucuuacga cgagaacggg gaaaucuucu gggauaaagc gcgccauauu     2400

ccucaaauca agaaagugau uagcucccac caggucaaua ucgugaagaa gguggagguu     2460

cagacgggag gcuuuuauaa agagaccguc aaccccaagg gcaaaccuga caagcugauc     2520

cagcggaaag ccggguggga ugugucuaag uacggaggcu ucgggagccc cguggucgcu     2580

uaugccgugg cuuuuauuua cgaaaaggga aaagccagaa agaaagccaa ggcuaucgag     2640

ggcauuacaa ucaugaaaca aucccuuuuc gaacaggacc cgauuggguu ucucagcaau     2700

aagggcuauu cuaacguuac caaguucauc aaacugucca aguacacacu guacgagcuc     2760

gaaaauggac gcagacggau gguggcgagc cauaaagagg cccagaaggc uaacucuuuc     2820

auucugccug agaaacuugu cacccugcuc uaucacgccc agcauuacga ugaaaucgcc     2880

cacaaggagu ccuuugacua ugugaaugau caccugagcg aguuccgcga gauccuggac     2940

caaguuauug auuuuucuaa cagauacacg aucgcugcca agaacaccga gaaaauugcg     3000

gaacuuuucg agcagaauca ggaaagcaca gugcaguccc ugagccaauc uuuuaucaac     3060

cucaugcagc ugaaugcuau gggcgccccc gccgacuuca aguuuuucga ugucauuauc     3120

ccucggaaac gcuaucccuc ccucaccgag aucugggaaa gcacaauuau cuaccagucc     3180

auuaccgggc ugagagagac gcggacacgc auggcuaccc uuugggacgg agagcaauga     3240


<210>  3862
<211>  3243
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3862
augaagacca gcuucgccuu ugacaucggc acaaacucca uuggguggug cgugcuggcu       60

cucgauaaug ccaaccugcc cuacagaauc cuugacgcgg gaguccggau uuucucugau      120

ggccgcgagc cuaaaagcgg gacgucccug gcugaaggaa gacggcucgc ccgcggcaug      180

ucuagacggc gcgacagaua uaagcggcgc agaaaagccg uuauccggac ccugauugag      240

uacgggcuua ugccggauga ccccgcugcc cgcagagcuc ugguggccga aacaaaugau      300

aaggcgccug gaaaaaucgc cagcgacgug uaugcucucc gggcccgcgc ucuggaugac      360

aagcugcccc uccaccauau cggcagagcc cuguuucaca uuaaccagcg gagaggcuuc      420

aaguccaauc gcaaaaccga uaagcgggac aacgaucaag ggaaaaucgc gucuggaauu      480

agcagacuuc gcgacaagcu ggccuccgag ggcaugcgga cacucgggga auaccugaau      540

cauagacacc aggcuggaaa cuggguccgc gugcggccua gcagaguucc cggcaaagac      600

gauaaggagg aggacgggua uggauuuuac ccugaacgcg cccuucugca ggaugaguuc      660

aaugcuaucu ggaccgccca ggccgcucau uauccggacg ugcucacaga agaacggaga      720

acccaccugg agacgauuau cuuuuuccaa cgcccccuga agccugucca ggugggcaaa      780

uguucuuuua accccggcga ggagcggcuc ccuagagcgc acccccuguu ccaggaauuu      840

cggcuuuaca aggagguuaa ugaacuggag cucguggauc gcaaccagga ccaucacaaa      900

cugucccuug aucaaagaga cgcccugauu gcucagcuca agacccagcg ggaggccgcc      960

uacagcgcuc ugagaaaaac agcccgcgcg ccgcugggga cccgguucaa uaaggaaaca     1020

gagucuagaa ccaaaaugaa gggagaugaa gucgcugccg agcucuccgc caagacgcgc     1080

uuuggccagg cuuggaucag caugccucug gaagagagau ggaucauugu ggacacacuu     1140

cggcgcgugg aggauccccu ggaacucacc gccuggcugg agcaacauua ugggcuugac     1200

ggagaacaca ucgacgcuau ucuggccgcg agacucccug agggcuacgg gcggcuggga     1260

gaaacagccc ugaccgagau ccucgucgag cugaaagcug ccguuauuuc ugaagcugag     1320

gccgcgcgca gagccggcua ugaucacgcc cuucugcagc ggggcgccgg gcucgaagcu     1380

cugcccaagu accaggaggu gaucgcccgc agaaucccuc ccggaaccgg caacccggac     1440

gaucccuaug acguggagaa agggcggauu acaaauccua cgguccauau cggacugaac     1500

caacuucaga aggugcucaa cgcccugauu cgcagauacg gcaaacccga acagaucgcu     1560

auugagcugg cgcgggaacu caagcugaau gaugagcaga agagagaaaa gaacaucgag     1620

aucgcccgca auacccgggc ugccgagcaa agauccgcca aacuuguuga acugggggug     1680

ccugacacag gcuauaaccg cguccuccug aaacuguggg aggaacuuaa ucuccgggcu     1740

cccgagaaca gagugugcau uuacugugga gaaccgauca auauugagac ccuguucagc     1800

uccgccguug auaucgacca cauucugccu uggucuaaga cacucgauga cagccaggcg     1860

aaccgccugg ugugccaugu cgcuuguaau cggcagaaag ccaacuacgc ccccgcugag     1920

gugccucagu ggcaaggcag auaugaugac auccuugccc gcgcgaauac ccugccccuc     1980

aacaagcggu ggagauucgg gcgggaugcu augaagaaau uugccgacga agagacguuc     2040

cuggcccgcc agcugacaga cacccaguac cuuuccagac ucgcucacgc cuauauggcu     2100

gcccuguacc cugaugagga acccgacgcg cagggagucc uggcccggaa gaaucaugug     2160

cgcguuauua ccggcagaau gacagaaaug cuccggagac aaugggggcu gaacagccuu     2220

cuggcugauc acaaucagaa aaaccgcaac gaccaccggc aucacgccau cgaugcugcc     2280

guggucggag ugaccacaag agcccuccug cagcgcaucg cuacgguugc gggcgggcgg     2340

gaggcccagg cucuggagga aaccauuggc aagaucgagc cgccuuggcc cggauuuaga     2400

gacgaacuuc aagccgugau uaaacgcauc gucgugucuc auaagcccga ucacggcaca     2460

cucccuaagc cggggcaggg aggccagacc gccgggcaac ugcacaauga cacagcuuau     2520

ggacugaccg gcgagacgga uucccggggg acccccauug ugguuagacg caaaccuuuc     2580

acaagccuca ccgagaagga ccugcccucu aucagagauc ggcagcuugc ccaggcgcug     2640

ggcagcgcua ucgccggacg cuccggcaaa gaacuccagg ccgcucugga gcaauuuaga     2700

gaccgggaug acuacaaguc uauucgccau gucagagugg uggaaccucu gaacgucauc     2760

cccauucggg acgggacagg cagagccuau aaagcguaca agggagaugc uaauuaccgc     2820

uaugacgugu gggagcuucc uaacgggaaa uggaugcacg aaguugugag cauguucgau     2880

gcccaugacc gguccuggac cagcgccguc cacauccauc accccgcugc cagacgcgug     2940

cugucucuca agcagaauga uaugguugcu uacgaccacc cgcaggaugg accuacgauu     3000

ggccgggugg ucaaguuuuc ccagagcggg caaaugacau ucgcucccca uaaagaggcg     3060

ggcgcccuga aggcuagaga cgccgauccu gaggaccggu uucgcuauuu cuacaaaucu     3120

ggauccgcua ugaaggauau cggccucaga caggugcggg uugacgaaau cgggcgcgug     3180

uuugaccccg gaccgagaga uaaagaggcc cggaacgccc gcaagaagaa aagcaccgac     3240

uga                                                                   3243


<210>  3863
<211>  3243
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3863
auggccaaga gcuucgcuuu ugacaugggc accaacucca ucgggugggc cgugcuggga       60

cucgauucug cgggcgaccc cgauagcauu gagggggcug gagucagaau cuucuccgac      120

ggccgggaac cuaaaucugg ggccagccug gccgagggac gcagaauugc ucggggcaug      180

ucccgcagac gggaacgcua caaggccaga cggaaagcuc uuauccgcac acugggcgau      240

uaugggcuca ugccgcugga gagagccgcg cggcgcgccc uuguugcuga aacgaaugac      300

agaucuuuug gacugguggc ccaggaugug uacgcucucc gggcccgcgc gcuggucgag      360

aagcugcccc cuuaucaccu cggcagagcc cuguuccauc uuaaccaacg gagaggguuu      420

aaaagcaauc gcaagaccga cggacgggau aacgagcugg gcaugauugc uacagggauc      480

gccgcucucg acgcccagcu gaauggaaga acccuuggcc aguaccugca cgaaaugcgc      540

ggcgccgauc cccggcagag agggcaagug cgcguucggc cucaucucga gaucaacgac      600

aagggaagag aaauugcugc guauggcuuc uacccccagc gcgccaugcu ggagcgggag      660

uuuaaugcua ucugggccgc ccaggcuccu caccauccgg aacugcucuc cgaggaaaga      720

cgcgcccacc uguuccaggu gaucuuuuau caacggcccc uuaaacagcc ugaucccggg      780

agaugcagcu ucaacccugc ggagccccgg cuggcuaagg cccacccgcu cuuucagcgc      840

uucagacugg ucaaagaaau uaaugagcuu cggauuguga caggaucuaa cucccagggc      900

guuccucuga cccucgagca aagagacauc cugcugacac uccugcgcga caagaaagcc      960

agcucuuuua agacccuucu ggcuaagauu aucaaagggc agcccgaagc ccgguucaau     1020

aaggcuuccg auagcagaga caaaaugaag ggagaugagc ucgccgcgac gaugucugac     1080

aaaacccgcu ucggccaggc cugguuugcu augccugaag ccgagcaaug gagaaucauu     1140

gcuaagcugc gggauaucga cgauccccuu gaacugaacg ccuggcucga guccgaguac     1200

ggccuggcgc ccgaacgcag agacgccgug gcuaaugccc ugcucccuca gggguacgga     1260

cggcugggcg agacagcucu uagaaccaug cuggauuauc ucgaagccgc cgucauuagc     1320

gaggcugaag cggccgcuca ggccggguac gaccaugccc uggcuagacg gggagccggc     1380

cuggaucagc uuccgaaaua ucaagaggug cucgggcgca gaaucccucc cggcuccgga     1440

gagcccaacg acccuuacga cauucggaug ggccgcaucu cuaaucccac agugcacauc     1500

gcgcugaacc agcugagacg ggucguuaac gcucucauua gaaaguaugg gaagccggcc     1560

gaaaucgcca uugagcuggc ucgcgaucuu aaacugagcg aagaacagaa gcgggaggug     1620

aaugccacca ucgcgaaaaa cacgagagac gcugaacgcc gggccgugag acucgccgau     1680

gagggaauug acaauacagg ccauaaccgc gcucugcugc gguucuggga ggagcuugcc     1740

gcugauccuc ucgacagagu cuguguguac ugcgggggag gcaucggggc cgcgucccug     1800

uuuaauggca gcguugauau cgaccacauu cugcccuggu cucgcacccu cgaugacucc     1860

cgggccaaca gaauccuggu gcacaauagc uguaaccggc uuaagcgcaa uuucgcuccu     1920

uuugaagucc ccgaguggag agagauuuau caggauaucc uggugcgggc cgcuaaacuc     1980

ccugccaaca agucuuggcg cuucgccccc gacgcuaugg auagauuuga aggagagaag     2040

gacuuccugg cgcggcaacu gacagacacc caguaccuug ccagaauggc ucaugaauac     2100

cucgccgccc uguauccgga ugaggaaccu gacgaguggg gcguuuuugc ucgcgggaau     2160

cacgugcggg ucgugcccgg aagacugacc gagaugcucc gccgggccug gggccugaac     2220

gcgauucuua gcgaugacaa ucgcaaaaac cgcaaugauc aucggcacca ugcuaucgac     2280

gccgucgugg uuggggugac aacgagagau augcugaaca agauuuccca ggccgcugga     2340

cgcggcgccg acaugggggc ggcugaugcc cucagacggu ucgauccccc uuggccgucu     2400

uuucaccagg ccguccgcgc ugccguggaa agaauccugg uuagccaccg ggcugaccau     2460

ggcacccugc ccggaacaga cggccaaggg uccaccgccg gacagcuuca caacgauaca     2520

gcguacggcc ucaccgggga gacgcgcaaa ggacuggccg uggucgugag acggaagccu     2580

uaucugagcc ucgaacccag agacaucgag acaauucgcg acccucagcu gcgggaucau     2640

cuugcuaccg aaaccuacgg ccuggccggg gcugaguuca cagccgcccu caaaagacug     2700

cagcaaaccg acaaguuuua ugcuggcauc cgccggguga gaguuacaga ggcgcugucu     2760

gucauuccca uccgggaugc cacgggacac gcuuacaagg ccuauaaagg cgacgccaau     2820

uuccgcuacg auguguggga acuuccuaac gggaaguggg ugcccgaggu cauuuccaug     2880

uucgacgcuc acgauagagg augggugagc cagguucaug ccgcgaaucc ggcugcccgg     2940

cgcgugcugu cucucagaca gaacgacaug gucgccuacc aacacccuga acagggccug     3000

guguaugggc ggguugugaa auuuggacag uccggccaga ugaccuucgc ugcccaucac     3060

gagggcggga gccucaaguc ccgcgaugcg gacagagaug accccuuuaa auacuucuau     3120

aagucugccg gaacaaugaa ggacaucggc cugcggcaaa uccgcgucga ugagauuggg     3180

agagugcagg acccuggacc ccaggaucgg gacagcaaaa gaguucgcaa guccgaucgg     3240

uga                                                                   3243


<210>  3864
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3864
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagacgaga acccgauuug ccugaucgau      120

cuuggcguua gaguguuuga acgggcugag gugcccaaaa ccggggacuc ccuggccaug      180

gcccgcagac ucgcucgguc uguccgcaga cugacacggc gcagagccca ccggcuucug      240

cgcgcuagac ggcuccugaa gcgcgaagga gugcugcagg ccgcggauuu cgacgagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuua gagccgcugc ccuggaucgg      360

aagcucaccc cucuggagug guccgcuguu cuucugcauc ucauuaaaca ccgcggguau      420

cugucucaga gaaagaauga aggagagaca gccgacaagg aacugggcgc gcuccugaaa      480

ggcguggccg auaacgcuca ugcccuucag accggggacu uucggacacc cgcuaagcug      540

gcccucaaua aauucgagaa ggagagcgga cacaucagaa accagcgcgg cgauuacucc      600

cauaccuuuu cucggaaaga ccugcaagcc gaacuuaauc uccuguucga gaagcagaag      660

gaauuuggga acccucacau uagcgaugga cugaaagagg gcaucgaaac acugcucaug      720

acccagagac cggcucuguc cggggacgcg guccagaaga ugcuuggaca cuguacguuc      780

gagcccaccg agccuaaagc cgcuaagaau acauauaccg ccgaacgcuu uguguggcug      840

acaaaacuca acaaucugcg gauucuugag caaggcagcg aacagccccu gaccgacacg      900

cagagagcca agcucaugga ugagccuuac cgcaaaucua agcugacaua ugcucaggcc      960

cggaagcugc ucggccugga agacaccgcg uucuuuaaag ggcuuagaua cggaaaggau     1020

aacgcugagg ccuccacacu gauggagaug aaagccuauc augcuaucag ccgcgcccuc     1080

gaaaaggagg gccugaaaga caagaagucu ccccugaauc uuuccccgga acuccaagau     1140

gagauuggga ccgcuuucag ccuguuuaaa accgacgaag auaucacagg acggcugaag     1200

gacagaguuc agccugagau ccucgaggcc cugcuuaaac acauuucuuu cgauaaguuc     1260

gugcagaucu cccugaaagc gcuccgccgg auuguccccc ugauggaaca aggcaagaga     1320

uacaacgagg ccugcgcuga aaucuacggg gaccauuaug gaaagaaaaa cacggaggag     1380

aagauuuacc ugccucccau ccccgccgau gaaauccgga auccuguggu gcuucgcgcu     1440

cucagccagg ccagaaaagu cauuaacggc guugugcgga gauauggcuc cccggcgcgc     1500

auccacauug agaccgcccg ggaagugggg aagucuuuua aagacagaaa ggagaucgaa     1560

aagcgccagg aggagaauag aaaagaccgg gaaaaggcug ccgcuaaauu ccaggaguac     1620

uuucccaacu ucgucggaga accuaagagc aaagauauuc ugaagcugcg ccucuaugag     1680

caacagcacg gcaaaugucu guacuccggg aaggaaauca aucuuggaag acugaacgag     1740

aagggcuaug uggagaucga ccaugcccuc cccuuuagcc ggacauggga ugacucuuuc     1800

aauaacaaag uucuggugcu gggguccgaa aaucagaaca agggcaauca gaccccuuac     1860

gaguacuuua acggaaaaga uaauagccgc gaauggcaag aguucaaggc cagagucgaa     1920

acaucucggu uuccccgcag caaaaagcag cggauucuuc uccagaaguu cgacgaggau     1980

ggcuuuaaag agagaaaccu gaaugacacc cgcuauguga acagauuccu gugccaguuu     2040

guugcugauc ggaugagacu cacggggaag ggcaaaaagc gcguguucgc guccaacgga     2100

caaaucacaa aucugcuucg gggguucugg ggacugagaa aaguccgcgc cgaaaacgac     2160

cggcaccaug cucucgaugc cguggucgug gccuguucua ccguugcuau gcagcagaag     2220

auuaccagau uugugcgcua caaggagaug aaugccuucg acggcaaaac aaucgauaag     2280

gaaaccgggg agguccugca ccagaaaaca cauuuuccgc aaccuuggga guucuuugcg     2340

caggaaguga ugauucgggu uuucggcaag cccgacggaa aaccugaguu ugaagaggcu     2400

gacacgcccg aaaagcugag aacccuucuc gccgagaagc ugagcucccg cccugaggcc     2460

gugcacgaau augucacacc ccuguucgug agccgggcuc cgaacagaaa aaugucuggc     2520

caggggcaca uggagaccgu gaaguccgcc aaacggcucg augaaggagu uagcguccug     2580

cgcgugccuc uuacacaacu gaagcucaaa gaccuggaga agauggugaa uagagaacgg     2640

gagcccaaac uguacgaggc gcuuaaggcu cgccuggaag cccauaagga ugaccccgcc     2700

aaggcuuuug ccgagccuuu cuauaaauac gauaaagcug gcaacagaac ccagcagguc     2760

aaggccgugc ggguugaaca ggugcagaaa acgggggucu gggugagaaa ucacaacgga     2820

aucgcggaca augccaccau gguucgcgug gaugucuuug agaagggcga caaguauuac     2880

cucgugccga uuuacucuug gcaaguugcu aaagggaucc ugcccgaucg ggccgugguc     2940

gcuuaugccg acgaagagga uuggacagug aucgacgagu ccuucagauu uaaguucgug     3000

cucuacagca acgaucugau uaaaguccag cuuaagaaag acuccuuccu gggcuauuuu     3060

ucuggacucg accgcgccac cggcgcuauc agccugcggg aacaugaucu ggagaagucc     3120

aaggggaaag acggaaugca cagaauuggc guuaagacag cgcuuucuuu ccagaaauac     3180

caaaucgaug aaauggggaa ggagauucgc ccuugccggc ugaaaaagag acccccugug     3240

cgcuga                                                                3246


<210>  3865
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3865
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagacgaga acccgauuug ccugaucgau      120

cuuggcguua gaguguuuga acgggcugag gugcccaaaa ccggggacuc ccuggccgcc      180

gcucgcagac ucgcccgguc uguccgcaga cugacacggc gcagagcuca ccggcuucug      240

cgcgccagac ggcuccugaa gcgcgaagga gugcugcagg cggccgauuu cgacgagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuua gauccgcugc ccuggaucgg      360

aagcucaccc cucuggagug gucugcuguu cuucugcauc ucauuaaaca ccgcggguau      420

cugagccaga gaaagaauga aggagagaca gccgacaagg aacugggcgc gcuccugaaa      480

ggcguggccg auaacgcuca ugcccuucag accggggacu uucggacacc cgcugagcug      540

gcccucaaua aguucgagaa agaauccgga cacaucagaa accgccgggg cgauuacucu      600

cauaccuuua gcagaaagga ccugcaggcc gagcuugauc uccuguucga aaaacaaaag      660

gaguuuggga auccucacau uuccgacgac cugaaggaag gaaucgagac acugcucaug      720

acccagcgcc cggcucugag cggcgaugcg guccagaaaa ugcuugggca cuguacguuc      780

gagcccaccg aaccuaaggc cgcuaaaaac acauauaccg ccgagcgguu uguguggcug      840

acaaagcuca auaaccugcg cauucuugaa cagggaucug agagaccccu gaccgacacg      900

gaacgggcca cacucaugga ugagccuuac agaaaaucca agcugaccua ugcucaagcc      960

cgcaaacugc ucgagcugga cgauacagcg uucuuuaagg gccuucggua cggcaaggac     1020

aaugcugaag ccagcacccu gauggagaug aaagccuauc augcuaucuc uagagcccuc     1080

gaaaaggagg ggcugaaaga uaagaaaagc ccccugaacc uuagcccgga acuccaggac     1140

gagauuggaa ccgcuuucuc ucuguuuaag acagaugagg acaucacggg ccgccugaag     1200

gaucggguuc agccugaaau ccucgaggug cugcuuaaac acauuuccuu cgacaaguuc     1260

guccagauca gccugaaagc ccucagacgg auugugcccc ugauggaaca agggaagcgc     1320

uacgacgagg cgugcgccga gaucuacgga gaucaugacg gcaaaaagaa uaccgaagag     1380

aagauuuauc ugccucccau ccccgcugau gaaaucagaa acccuguggu ccuucgggcc     1440

cucucccagg cuagaaaagu uauuaacgcc guggugcgcc gguacggguc uccggcgaga     1500

auccacauug agacagcccg cgaagucgga aagagcuuua aagacagaaa ggagaucgag     1560

aaacggcagg aagagaaucg caaggauaga gaaaaggcug ccgcuaaauu ccgggaguau     1620

uuucccaacu ucgugggcga accuaagucc aaagacauuc ugaagcugcg ccucuacgag     1680

caacagcacg gcaaaugucu guauagcggg aaggagauca aucuuggaag acugaacgaa     1740

aaaggcuacg uugagaucga ucaugcccuc cccuuuucuc ggaccuggga cgauuccuuc     1800

aauaacaagg ugcugguccu ggggagcgaa aaucagaaca agggaaauca gacaccuuau     1860

gaguacuuua acggcaaaga caauucucgc gaauggcaag aguucaaggc cagaguggag     1920

accagccggu uuccccgcuc caaaaagcag cggauucuuc uccagaaauu cgaugaagag     1980

ggguuuaagg aaagaaaccu gaaugacacg agauacguua accgcuuccu gugccaguuu     2040

guggcugauc acaugcuccu gacaggcaag ggaaaacgga gagucuucgc gucuaauggc     2100

caaaucacca accuucugcg cggguucugg ggacuccgga aggugagagc cgagaacaau     2160

cgccaucacg cucuggacgc cgucgugguu gccuguagca ccguggcuau gcagcagaaa     2220

auuacacggu uugucagaua uaaggagaug aacgccuucg acggcaaaac caucgauaag     2280

gaaacagggg aggugcugca ucagaagacg cacuuuccgc aaccuuggga guucuuugcg     2340

caggaaguua ugauucgcgu guucggaaaa cccgacggca agccugaauu ugaggaggcu     2400

gauacccccg aaaaacuucg gacacuccug gccgagaagc uguccagcag accugaagcc     2460

guccacgagu acgugacacc ccucuucgug ucucgggcuc cgaaucgcaa aauguccggg     2520

cagggccaua uggaaaccgu uaagagcgcc aagagacugg acgagggagu cucugugcuu     2580

cgggugccuc ugacccagcu caaacugaag gaucuggaga aaauggucaa ccgcgaaaga     2640

gagcccaaac uuuaugaagc gcugaaagcu cggcucgagg cccacaagga cgaucccgcc     2700

aaggcuuuug ccgaaccuuu cuacaaguau gacaaggcug gcaauagaac gcaacaggug     2760

aaagccguuc gcguggagca gguccaaaag accggggugu ggguucggaa ccauaaugga     2820

aucgcggaua acgccacaau ggugagaguc gacguguuug agaaagcugg caaguacuau     2880

cugguuccga uuuacuccug gcaaguggcc aaagggaucc uccccgaucg cgcugucgug     2940

gccuacgccg acgaagaggg auggaccgug aucgaugaau ccuuccgguu uaaguucguc     3000

cuguauucca augaccuuau uaagguucag cugaaaaagg acucuuuccu cggcuacuuu     3060

agcgggcugg auagagcuac aggcgcgauc ucccugcgcg agcacgaccu ugagaaaucu     3120

aagggaaaag auggcaugca ccggauuggg gugaagaccg cccugagcuu ccagaaguau     3180

cagaucgacg aaaugggaaa agagauuaga cucugccgcc ugaagaaaag accucccgug     3240

cgguga                                                                3246


<210>  3866
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3866
augaccgccu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcu       60

agcgugggau gggccauggu cgagaucgau gaagaggaaa acccgauuag acugaucgac      120

cuuggcguuc ggguguuuga gcgcgcggaa gugcccaaaa caggggauuc ccuggcugcc      180

gccagacggc ucgcucgcuc ugucagacgg cugacgcgca gacgggccca ccgccuucug      240

agagcucggc gccuccugaa gagagaggga gugcugcagg ccgcggacuu cgaugagaau      300

ggccucauca aaagccugcc uaacaccccc uggcaacuuc gggccgcugc ccuggaccgc      360

aagcucacac cucuggaaug guccgcuguu cuucugcauc ucauuaaaca cagaggguau      420

cugucucagc ggaagaauga gggagaaacc gccgauaagg agcugggcgc gcuccugaaa      480

ggcguggccg acaacgcuca ugcccuucag acaggggauu uuagaacccc cgcugagcug      540

gccgucaaua aguucgaaaa agagagcgga cacauccgca accagcgggg cgacuacucc      600

cauacauuuu cuagaaagga ucuccaagcc gaacuggugc uucuguucga gaaacagaag      660

gaauuuggga auccucacau uagcgacgga cucaaggagg gcaucgagac ccugcugaug      720

gcucagcgcc cggcgcucuc cggggaugcc guucagaaaa ugcugggaua uugcacguuc      780

gaacccaccg agccuaaggc ugccaaaaac acauacaccg ccgaacgguu uauuuggcuu      840

acaaagcuga auaaccucag aauccuggag caaggcagcg aacgcccccu uaccgacacg      900

gagcgggcua cacugauuga caugccuuau aaaaauaagc ucaccuacgc caaagcgaga      960

gagcugcugg gccucgauga cacagcuuuc uuuaaggggc ugcgcuaugg aaaggauaac     1020

gccgaaucug ccacccuuau ggagaugaag gcuuaccacg ccauccggag agcucuggaa     1080

aaagagggcc ucaaagacaa gaaaagcccc cugucccugu cuccggaacu ucaggaugag     1140

aucgggaccg ccuucucccu cuuuaagaca gacgaggaua uuacgggacg ccugaagggc     1200

ugucugcagc cugaaauccu cgacgcgcug cuuaaacaua uuaauuucga uaaguucgug     1260

cagaucagcc ugaaagcccu ccggagaauu gucccccuga uggagcaagg gaagcgguac     1320

gacgaggcuu gcgccgaaac cuauggagau cacuacggcg agaaaaacuc ugaagagaug     1380

cuguaucuuc cucccauccc cgcugacgaa auccgcaacc cuguggugcu cagagcccug     1440

ucccaggcgc ggaaggucau uaauggcguu gugagacgcu acgggagccc ggcccggauc     1500

cauauugaga cagcuagaga agugggaaag uccuuuaaag accgcaagga gaucgagaaa     1560

agacaggaag agaaccggaa ggaucgcgaa aaagccgcug ccaaguucag agaguauuuu     1620

cccaauuucg ucggcgaacc uaagucuaaa gacauucuga agcuccggcu guacgagcaa     1680

cagcacggga aaugucuuua uagcggaaag gccaucgaua uccgcagacu gaacggcaaa     1740

ggguacgugg agauugacca cgcucucccc uuuucccgga ccugggauga cagcuucaau     1800

aacaagguuc uggugcuggg cucugaaaau cagaacaaag gaaaucgcac accuuacgag     1860

uaucuuaacg gcaaggauaa uuccagagaa uggcucgagu uuaaggcgcg ggucgaaacc     1920

agccgcuucc cccauucuaa aaagcagaga gugcugcugc aaaaauuuga cgagaagggg     1980

uucaaagagc ggaaccucaa ugauacgaga uacguuaacc gcuuucugug ccaguucgug     2040

gccgaccaca ugcagcuuac aggaaagggc aagcggagag ucuuugcuag caaugggcag     2100

aucaccaacc ugcuccgcgg auucuggggc cugcauaaag ugcgggccga aaacgauaga     2160

caccaugccc uggacgcugu cgugguugcc uguuccaccg uggcgaugca acagaagauu     2220

acaugcuucg uccgcuauaa agagaugaau gcuuuugaug ggaagaccgu ggacaaagaa     2280

acaggcgagg uucuucacca gaagacgcac uucccgcagc cuugggaguu uuucgcccaa     2340

gaagugauga uccgggucuu uggaaagccc gacggcaaac cugaguucga agaggccgau     2400

acccccgaaa agcucagagc ucugcuggcc gagaaacucu cuagccgccc ugaggcggug     2460

caugaauacg ugacaccccu guuuguuucc cgggcuccga acagaaagau gagcggggcc     2520

cacaaagaca cccuucgguc ugccaagcgc uucgucaagc auaaugagaa aauuuccgug     2580

aagagagugu ggcugacaga aaucaaacuc gcugaucugg agaacauggu caauuauaag     2640

aacggacggg aaauugagcu guacgaggcc cuuaaagcuc gccugggcgc cuauggggga     2700

aaugcgaagc aggccuuuga cccuaaagau aaccccuucu acaagaaggg cgggcagcuc     2760

gugaaagcug uuagagugga aaagacccag gagagcggcg uccugcucaa uaaaaagaac     2820

gccuacacga ucgcugacaa uggagauaug gugcggguug acguguuuug uaaagucgau     2880

aagaagggca aaaaccaaua uuucaucgug cccauuuacg ccuggcaggu ugccgaaaau     2940

auccugccug acauugauug caagggguau agaaucgacg auucuuacac cuuuuguuuc     3000

ucccuucaca aauaugaccu gauugcuuuc cagaaggacg agaaaagcaa gguggaauuu     3060

gcguacuaua ucaacugcga uuccucuaau ggacgcuucu accucgccug gcacgacaag     3120

ggcagcaaag agcaacaguu ucggaucucc acacagaacc ugguccugau gcagaaguac     3180

caaauugaug agcuugggaa agaaaucaga ccgugucgcc ugaagaaacg gcccccugug     3240

agauga                                                                3246


<210>  3867
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3867
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagacgaga acccgauuug ccugaucgau      120

cuuggcguua gaguguuuga acgggcugag gugcccaaaa ccggggacuc ccuggccgcc      180

gcucgcagac ucgcccgguc uguccgcaga cugacacggc gcagagcuca ccggcuucug      240

cgcgccagac ggcuccugaa gcgcgaagga gugcugcagg cggccgauuu cgacgagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuua gauccgcugc ccuggaucgg      360

aagcucaccc cucuggagug gucugcuguu cuucugcauc ucauuaaaca ccgcggguau      420

cugagccaga gaaagaauga aggagagaca gccgacaagg aacugggcgc gcuccugaaa      480

ggcguggcca acaaugcuca ugcccuucag accggggauu uucggacacc cgcugagcug      540

gcccucaaca aguucgagaa agaauccgga cacaucagaa aucagcgcgg cgacuacucu      600

cauaccuuua gccggaagga ucugcaagcc gagcuuauuc uccuguucga aaaacagaag      660

gaguuuggga acccucacgu cuccggaggc cugaaggaag ggaucgagac acugcucaug      720

acccagagac cggcucugag cggagacgcg gugcagaaaa ugcuuggcca cuguacguuc      780

gagcccgccg aaccuaaggc ugccaaaaau accuauacag ccgagcgcuu uauuuggcug      840

accaagcuca acaaucugcg gauccuugaa caaggcucug agagaccccu gacagauacc      900

gaacgcgcua cgcucaugga cgagccuuac cggaaaucca agcugacaua ugcccaggcg      960

agaaaacugc ucgggcugga ggacaccgcu uucuuuaagg gacuucgcua cggcaaggau     1020

aacgccgaag ccagcacacu gauggagaug aaggcuuauc augccauuuc ucgggcucuc     1080

gaaaaggagg ggcugaaaga caagaaaagc ccccugaauc uuuccccgga acuccaggau     1140

gagaucggaa ccgccuucuc ucuguuuaag accgacgagg auaucacagg cagacugaag     1200

gaccgcauuc agccugaaau ccucgaggcg cugcuuaaac acauuuccuu cgauaaguuc     1260

guucaaauca gccugaaagc ccuccggaga auugugcccc ugauggaaca ggggaagcgg     1320

uacgacgagg cuugcgccga gaucuacgga gaucauuaug gcaaaaagaa cacggaagag     1380

aagaucuacc ugccucccau ucccgcugac gaaauccgca acccugucgu gcuuagagcc     1440

cucucccagg cgcggaaagu gauuaauggc gucguuagac gcuauggguc uccggcccgg     1500

auccacauug agaccgcuag agaaguggga aagagcuuua aagaucgcaa ggagaucgag     1560

aaaagacaag aagagaaccg gaaggaccgc gaaaaggccg cugccaaauu cagagaguac     1620

uuucccaauu ucgugggcga accuaagucc aaagacaucc ugaagcugcg gcucuaugag     1680

cagcagcacg ggaaaugucu guacagcgga aaggagauua accuuggccg ccugaaugaa     1740

aaaggguaug ucgagaucga ucaugcccuc cccuuuucua gaacauggga cgauuccuuc     1800

aacaauaagg ugcugguucu gggcagcgaa aaccagaaua agggaaacca aaccccuuac     1860

gaguacuuua auggcaaaga caacucucgg gaauggcagg aguucaaggc ucgcguggag     1920

acaagcagau uuccccgguc caaaaagcag cgcauucuuc uccagaaauu cgaugaagac     1980

ggguuuaagg agagaaaucu gaacgauacc cgguauguca auagauuccu gugccaauuu     2040

guggcggacc gcaugcggcu cacgggaaag ggcaaaaaga gaguuuucgc cucuaacggg     2100

cagaucacaa accugcuucg cggauucugg ggccugcgga aagugagagc ugaaaaugau     2160

cgccaccaug cccucgacgc cgucgugguc gcuuguagca ccguggccau gcagcagaag     2220

auuacccggu uuguuagaua caaagagaug aacgcguucg augggaagac aaucgacaag     2280

gagaccggcg aagugcugca ccagaaaaca cauuuuccgc agccuuggga guucuuugcu     2340

caggaaguca ugauucgcgu guucggaaag cccgauggca aaccugaguu ugaagaggcc     2400

gacacgcccg agaagcugcg gacccuucuc gccgaaaaac uguccagcag accugaggcu     2460

guucacgaau augugacacc ccuguucguc ucucgggccc cgaaucgcaa gauguccggg     2520

cagggacaca uggagaccgu gaaaagcgcg aaaagacucg acgaaggcgu gucuguucug     2580

cgggucccuc uuacacaacu gaagcucaaa gaucuggaga agauggugaa ccgcgagaga     2640

gaacccaaac uguacgaggc ucuuaaggcc cggcuggaag cccauaaaga cgaucccgcu     2700

aaggccuuug cugagccuuu cuauaaguac gacaaagccg ggaauagaac ccaacaggug     2760

aaggcggucc gcguggaaca aguucagaaa acgggagugu ggguccggaa ccacaauggc     2820

aucgccgaua acgcuaccau ggugagaguu gacguguuug agaaggccgg gaaauauuac     2880

cucgucccga ucuacuccug gcaaguggcu aagggcauuc ugcccgaucg cgccguugug     2940

gccuaugcug acgaggaagg auggacaguc aucgaugaga gcuuccgguu uaaguucgug     3000

cucuacucca augaccugau uaaagugcag cuuaagaaag auucuuuccu gggcuauuuu     3060

agcgggcucg acagagcgac cggagccauc ucccugcgcg aacaugaucu ggagaagucu     3120

aaaggcaagg acgggaugca ccggauugga gucaagacag cucuuagcuu ccagaaauac     3180

cagaucgacg agaugggcaa ggaaaucaga cugugccgcc ucaaaaagag accucccguu     3240

cgguga                                                                3246


<210>  3868
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3868
augcugaaga gagugcucgg caucgacguc gggauuagcu ccaucggaug ggcccugcuu       60

gaguuccagg gcgauauuga caacaaagau cugcccucug ggaagauccu cgcuagcgga      120

guuagagugu uuacccaagc cgaaaauccu aaaaacggcg agucccuggc gaagccgcgc      180

agagacgcuc ggcuugcccg cagacggaca cgcagacggg cccagcgccu gacggauauu      240

aaagaccucu ucuuuaagua caagcuggug accgggaucu cuagaaauaa aaucaacagc      300

auuuauaaga cagaagagaa uaaaaaggau cccuggaccc ugcggaaaga agcucucuac      360

cgcaagcugg acggagagga guucgccaga guccuuacac acaucgcuaa gcaucggggc      420

uuuaaaucca ugcgcaaguc ugaagaggaa ggcagcgaua ccaaagccaa ggagacacag      480

agagugcuga ccgagauugg gaaaaacaag gacacgcuca aaaccggaau cuggcagaca      540

gcgggcgaaa ugauugccuc ccggccuaag ggggaugcua gacgcaauaa gaccgacagc      600

uauaaaaacu cuaucucccg ggagcugcuu caagaugaaa caaagauuau cuucgagaaa      660

cagagaggaa ugaauagcca guuuaccucu gccgaccugg aaaaggagua cuccgacauc      720

gcuuuccacc aggcccccau ucaaagcaug cucaaaaugg uuggcgggug cacguuugag      780

aaggaugaag agggaaagcg ccugaaacgg gcgccuaaga acucuuauuc cgccgaagug      840

uucacagcuc ugaccaaaau caauaaccuc guccugaaga caaaaaccgc caauauuaac      900

gacagauuuc uuaccacaga ggaacgcaag aaggcucugg agcucuguca uagcaaugcc      960

aaagugacgu ucaagcagcu gcggggcgag cuuggccugc ccgaagauug gaucuuuaac     1020

accguugacu acgccaaaaa gccuaaaaag acagcuguga aggagaccga accggagucc     1080

gcgugcuucu uccagaugag aggguauaac gaaauucgca aaucugucgg aaaggaucuc     1140

uggacagaga ucagcaugaa aaaggagauc cucaauucca uugugaccac gcuggccaca     1200

gagaaaagcg acgaagcuau caaggcccag cugaaaaagu cugaagugcc cgaugagauu     1260

gccgagaagg uccugggccu uaacuuucgg ggguuccugc accucuccau caaagcucug     1320

gacaagauua ucccuuuuau gauggccgga gauaaauaca ccgacgcgug ugauaaggcu     1380

ggcuacaaag aacaaaucag cgggauuaag aagaucuucc uuagaaaacu gaaugacgag     1440

gaaaacgagc agauuaccaa ucccguugcc aagcgcgcca ucacacaguu ucggaaagug     1500

auuaacgcua ucauuagaga auauggaaag uucgaccaaa ugaauaucga gcucgcccgg     1560

gaucuggcua aaucuuacga cgaucgcaag aagaucaaag agggccagga agacuuuaga     1620

gccgagaagg aucgggcggc ccagagaugc aaagacaacg gccuggaucc uaauagcaag     1680

gacaaccucu ccaaauaucg ccugugggaa cagcaagaua auggguucug uauuuacugc     1740

ggaaagccca ucgagcuuca gcggcuggcu gaacagggcu auugugaggu ggaccauauu     1800

cucccguacu cuagaagccu ggaugacucc cagcacaaca agguccuggc ccacauccau     1860

gauaaucaac agaaaaagaa ccagauuccu uuugagcacu ucgggggaga cggccccaaa     1920

ugggaagagu uuguggggcg ccuuaauagc caguucaaga accucccuaa aucuaagcug     1980

aacagacugg uuaaacggaa guuugacgaa aaggauaugg agggcuucaa agaacgcaau     2040

aucaacgaca ccagauauau cucccgguuc auuaagaauu uuguggagaa ccaucucgag     2100

uucgcugaaa gcgcccaaaa acagaagguc uuugugcgcu cuggaucccu gacagcguuc     2160

cuuagacacc aguggggccu gaucaaaaag cgggaugaga gcgaccgcca ccaugcccuc     2220

gaugcucugg uuacggccug cgcuacccag gccaugguga aguccacagc cauuaccgcu     2280

cagaaaaugg aaucuuacaa uuggaucaac aaggagaaac ccagaaugcc ccugccuugg     2340

cuuaauuuuc gggacgaugu ccaaaagagc cucgacgcgg uguucgucuc ccgcgccccg     2400

agacacaaag uugggggagc ugcccauaag gagacaauuc ggucuaucag aaaguguggc     2460

gaagagaacc ugaucauuga gaaaacgccc cugguggaua ucgccaccaa gagcgggauu     2520

aaacucaagg acaucgaaaa acuguacaag aaggaacaga aucgcaaacu uuaugagacc     2580

cugaaggagc ggcucgaaca aaacggaaau gauccuguga aggcuuuugg caaccccgac     2640

gauccuguca gaaugcccac aaaugaccug accaagacag ggccgauuau caaauccauc     2700

aagcugauua ccaacaagaa aagcggccuu aaugugcgcg ccggaaucgc ggacaacggc     2760

gagauugcuc ggcucgaugu uuacgaaaga aaggggaaau ucuaucugau cccuaacuac     2820

guggccgacu uugccaauaa ggagcugccc aaaaaggcua uuaaagccaa cacgccugaa     2880

acagaguggc ccaucauuga ucgcaauuau aacuucaagu uuucucucuu caaggacgau     2940

cugaucucca ucaaacagaa acuugacagc ccugaggaau uuuacuauua caaaggaccc     3000

gaugucucua ccgcguccau uacaguggag agcguggacc ggaagagacu gugggaagag     3060

aaaaagaagg gcaaaaucga aaauguuuuc cacacccgca uugggaccaa gacacucaaa     3120

ucuuuccgga aguuuaaccu ggauauccug ggaacgaaag cuccggucga gauuaagaag     3180

gagaccuggg ugggcgugau ccggaaaaau ggggucgcca acaauacagg caacaaggcc     3240

auguga                                                                3246


<210>  3869
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3869
auggccgcuu ucaagcccaa cccuaugaau uacauccugg gccucgacau ugggaucgcc       60

agcgugggau gggcgauggu cgagauugau aaagaagaga acccgaucag acugauugac      120

cuuggcguuc ggguguuuga acgcgcugag gugcccaaga ccggggauuc ccuggccaug      180

gccagacggc ucgcucgcuc ugucagacgg cugacacgca gacgggccca ccgccuucug      240

agagcucggc gccuccugaa aagagaagga gugcugcagg acgccgauuu cgacgagaau      300

ggccucguua agagccugcc uaacacgccc uggcaacuuc gggcggccgc ucuggaucgc      360

aaacuuaccu gccuggagug guccgccgug cuucugcauc ucaucaagca cagaggguau      420

cugucucagc ggaagaauga aggagagaca gcugacaaag aacugggcgc ccuccugaag      480

ggcgucgcgg auaacgccca ugcucuccag accggggacu uuagaacccc ugccgagcug      540

gcucucaaua aauucgagaa ggaaagcgga cacauccgca accagcgggg cgauuacucc      600

cauacauuuu cuagaaaaga ccugcaagcc gagcuuaauc ugcuuuucga aaagcagaag      660

gaguuuagca acccccacgu guccgauagc cugaaagaag ggauugagac acugcucaug      720

gcccagcgcc cugcucuguc uggagacgcg guucagaaga ugcuuggcca cuguaccuuc      780

gagccggccg aacccaaagc ugccaagaau acguauaccg ccgagcgguu uaucuggcug      840

acaaaacuca acaaucugcg cauucuugaa caaggguccg agagaccucu gaccgacaca      900

gaacgggcua cccucaugga ugagcccuac agaaagagca aacugacgua ugcccaggcg      960

cgcaagcugc ucggacugga ggacacagcu uucuuuaagg gccuucggua cggcaaagau     1020

aacgccgaag ccccuacccu gauggagaug aaggcuuauc augccaucuc uagagcucuc     1080

gaaaaagagg ggcugaagga caaaaagagc ccccugaauc uuuccacaga acuccaggau     1140

gagauuggaa ccgccuucuc ucuguuuaag accgacaaag auaucacagg ccgccugaag     1200

gaccgggugc agccggagau ucucgaagcg cugcuuaaac acaucuccuu cgauaaguuc     1260

guccaaauca gccugaaagc ccucagacgg auugugccuc ugauggagca ggggaagcgc     1320

uacgacgagg cuugcgccga gaucuacgga gaucauuauu guaagaaaaa cgcugaggaa     1380

aagauuuacc ugcccccuau ccccgccgac gagauuagaa accccguggu ccuucgggcg     1440

cucucccagg ccagaaaagu uaucaauugc guggugcgcc gguauggcuc uccugcuaga     1500

auccacauug aaacggcccg cgaggucgga aagagcuuua aagacagaaa ggaaaucgag     1560

aagcggcaag aggaaaaccg caaagauaga gagaaggcug ccgccaaauu ccgggaauac     1620

uuuccgaauu ucguggggga gcccaagucc aaagacauuc ugaagcugcg ccucuaugaa     1680

cagcagcacg gcaaaugucu guacagcggc aaggagauca accuuguuag acugaaugag     1740

aagggguaug uggaaauuga ucaugcucuc ccuuuuucuc ggaccuggga cgauuccuuc     1800

aacaauaaag uccuggugcu gggaagcgag aaccagaaua agggcaacca aacacccuac     1860

gaauacuuua augggaaaga caacucucgc gaguggcagg aguucaaggc gagaguugaa     1920

accagccggu uuccucgcuc caaaaagcag agaauccuuc uccagaaguu cgaugaggaa     1980

ggauuuaaag agcggaaucu gaacgacaca agauauguga aucgcuuccu gugccaauuu     2040

gucgccgauc acauccuccu gaccggcaag gggaaacgga gaguguucgc uucuaacggc     2100

cagauuacga accuucugcg cggauucugg ggccuccgga aggucagagc cgaaaaugac     2160

cgccaucacg cccuggaugc ugugguugug gccuguagca cagucgcgau gcagcagaaa     2220

aucacccggu uugugagaua caaggagaug aacgcuuucg acgggaagac cauugauaaa     2280

gagacaggag aaguucugca ucaaaaggcc cacuuucccc agccguggga guucuuugcc     2340

caggaaguga ugauccgcgu cuucggcaaa ccugacggga agcccgaguu ugaagaggcu     2400

gacaccccug agaaacuucg gacacuccug gccgaaaagc uguccagcag acccgaggcg     2460

gugcacgaau augugacgcc ucucuucguu ucucgggcuc ccaaucgcaa gauguccgga     2520

cagggccaua uggagaccgu caaaagcgcc aagagacugg augaagggau uucugugcuu     2580

cgggugccgc ugacacaacu caaacugaag ggccuggaga aaauggucaa ccgcgagaga     2640

gaaccuaagc ucuacgacgc ccugaaagcu cagcucgaga cccacaagga uaaucccgcc     2700

aaggcuuuug ccgaacccuu cuauaaauac gacaaggcgg gaaaccggac acagcaagug     2760

aaagccguua gaaucgaaca ggugcagaag accggcgucu gggugcgcaa ucauaacggg     2820

auugcugaua augccacgau gguucgggug gacgucuuug aaaaaggagg caaguauuac     2880

cuggugccua ucuacuccug gcagguugcu aaggggaucc ucccggauag agccgugguc     2940

gccuucaaag acgaggagga uuggaccgug auggacgaua gcuuugaguu ccgcuucgug     3000

cuguaugcua acgaccuuau uaagcugaca gcgaaaaaga augaauuucu cggauacuuc     3060

gucucccuga accgggccac cggcgcuauc gauauuagaa cacacgacac cgacucuacg     3120

aaagggaaga auggcaucuu ucaaagcguu ggagugaaga cagcccuguc cuuccagaaa     3180

aaccagauug augaacuugg caaggagauc cgccccugcc ggcugaaaaa gagaccuccc     3240

gugcgcuga                                                             3249


<210>  3870
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3870
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagaggaaa acccgauuag acugaucgac      120

cuuggcguuc ggguguuuga gcgcgcugaa gugcccaaaa ccggggauuc ccuggccaug      180

gccagacggc ucgcucgcuc ugucagacgg cugacacgca gacgggccca ccgccuucug      240

agagcucggc gccuccugaa gagagaggga gugcugcagg ccgcggacuu cgaugagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuuc gggccgcugc ccuggaccgc      360

aagcucaccc cucuggaaug guccgcuguu cuucugcauc ucauuaaaca cagaggguau      420

cugucucagc ggaagaauga gggagaaaca gccgauaagg agcugggcgc gcuccugaaa      480

ggcguggcca acaaugcuca ugcccuucag accggggacu uuagaacacc cgcugagcug      540

gcccucaaca aguucgaaaa agagagcgga cacauccgca aucagcgggg cgauuacucc      600

cauaccuuuu cuagaaagga ccugcaagcc gaacuuauuc uccuguucga gaaacagaag      660

gaauuuggga acccucacgu cagcggaggc cugaaggagg ggaucgagac acugcucaug      720

acccagcgcc cggcucuguc cggagaugcg gugcagaaaa ugcuuggcca cugcacguuc      780

gaacccgccg agccuaaggc ugccaaaaau accuauacag ccgaacgguu uauuuggcug      840

accaagcuca acaaucugag aauccuugag caaggcagcg aacgcccccu gacagacacc      900

gagcgggcua cgcucaugga ugagccuuac agaaaaucua agcugacaua ugcccaggcg      960

cgcaaacugc ucgggcugga agacaccgcu uucuuuaagg gacuucggua cggcaaggac     1020

aacgccgagg ccuccacacu gauggaaaug aaggcuuauc augccauuag cagagcucuc     1080

gagaaggaag ggcugaaaga uaagaaaucu ccccugaauc uuuccagcga gcuccaggac     1140

gagaucggaa ccgccuucuc ucuguuuaag accgaugaag acaucacagg ccgccugaag     1200

gaucggguuc agccggagau ucucgaagcg cugcuuaaac acaucuccuu cgacaaguuc     1260

gugcaaauua gccugaaagc ccucagacgg aucgucccuc ugauggagca ggggaagcgc     1320

uacgaugagg cuugugccga aauuuacgga gaccauuaug gcaaaaagaa cacggaggaa     1380

aagaucuacc ugcccccuau ccccgcugau gagauuagaa accccguggu gcuucgggcc     1440

cucucccagg cgagaaaagu caucaauggc guugugcgcc gguauggguc uccugcccgc     1500

auucacaucg aaaccgcuag agagguggga aagagcuuua aagacagaaa ggagauugaa     1560

aaacggcaag aggaaaaccg caaggauaga gagaaggccg cugccaaauu ccgggaauac     1620

uuuccgaauu ucgucggcga gcccaagucc aaagacaucc ugaagcugcg ccucuaugag     1680

cagcagcacg ggaaaugccu guacagcgga aaggaaauca accuugugag acugaaugag     1740

aaaggcuaug uugaaauuga ccaugcccuc ccuuuuucuc ggacauggga ugacuccuuc     1800

aacaauaagg ugcugguccu ggggagcgag aaccagaaua agggcaacca aacccccuac     1860

gaauacuuua auggaaaaga uaacucucgc gaguggcagg aguucaaggc uagaguggaa     1920

acaagccggu uuccucgcuc caaaaagcag agaauccuuc uccagaaauu cgacgaggau     1980

ggcuuuaagg aauguaaucu gaacgacacc cgguauguua auagauuccu gugccaauuu     2040

guggcggauc acauucuccu gacggggaag ggaaaacgcc gggucuucgc cucuaacggc     2100

cagaucacaa accuucugag aggguucugg ggacuccgca aggugcgggc ugagaaugac     2160

agacaucacg cccuggaugc cgucgugguu gcuuguagca ccguggccau gcagcagaaa     2220

auuacccgcu uuguccggua caaggagaug aacgcguucg acggcaaaac aaucgauaag     2280

gaaaccggga aggugcugca ucagaaaaca cacuuucccc agccguggga guucuuugcu     2340

caggaaguua ugauuagagu guucggcaag ccugacggaa aacccgaguu ugaagaggcc     2400

gauacgccug agaaacuucg cacccuccug gccgaaaaac uguccagccg gcccgaggcu     2460

guccacgaau augugacacc ucucuucgug ucuagagccc ccaaucggaa gauguccggc     2520

caggggcaua uggagaccgu uaagagcgcg aaacgccugg acgaaggagu cucugugcuu     2580

agagugccgc ugacacaacu caagcugaaa gaccuggaga agauggucaa ccgggagcgc     2640

gaaccuaaac uuuacgaggc ucugaaggcc agacucgaag cccacaaaga ugaccccgcu     2700

aaggccuuug cugagcccuu cuauaaguac gauaaagccg gcaaucggac ccaacaggug     2760

aaggcgguua gaguggaaca aguccagaaa acgggggugu ggguucgcaa ccauaaugga     2820

aucgccgaca acgcuaccau ggugcggguc gauguguuug agaagggcga caaauauuac     2880

cugguuccua ucuacuccug gcaaguggcc aaggggauuc ucccggauag agcugucgug     2940

cagggcaagg acgaggaaga uuggcagcug aucgacgaua gcuucaauuu uaaauucucc     3000

cuucacccca acgaccuggu ggaggucauu acaaagaaag cccgcauguu cggauauuuu     3060

gccucuugcc accggggcac cgggaauauc aacauuagaa uccaugaucu cgaccacaag     3120

aucggaaaga auggcauucu ggaagggauc ggaguuaaaa cagcucugag cuuccagaag     3180

uaccagauug acgagcuugg caaagagauc cgcccuuguc ggcugaagaa aagacccccu     3240

gugcgcuga                                                             3249


<210>  3871
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3871
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagacgaga acccgauuug ccugaucgau      120

cuuggcguua gaguguuuga acgggcugag gugcccaaaa ccggggacuc ccuggccaug      180

gcccgcagac ucgcucgguc uguccgcaga cugacacggc gcagagccca ccggcuucug      240

cgcgcuagac ggcuccugaa gcgcgaagga gugcugcagg ccgcggauuu cgacgagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuua gagccgcugc ccuggaucgg      360

aagcucaccc cucuggagug guccgcuguu cuucugcauc ucauuaaaca ccgcggguau      420

cugucucaga gaaagaauga aggagagaca gccgacaagg aacugggcgc gcuccugaaa      480

ggcguggccg auaacgcuca ugcccuucag accggggacu uucggacacc cgcugagcug      540

gcccucaaua aguucgagaa agaaagcgga cacaucagaa accagcgcgg cgauuacucc      600

cauaccuuuu cucggaagga ccugcaagcc gagcuuauuc uccuguucga aaaacagaag      660

gaguuuggga auccucacgu cagcggaggc cugaaggaag ggaucgagac acugcucaug      720

acccagagac cggcucuguc cggagaugcg gugcagaaaa ugcuuggcca cuguacguuc      780

gagcccgccg aaccuaaggc ugccaaaaac accuauacag ccgagcgcuu uauuuggcug      840

accaagcuca auaaccugcg gauccuugaa caaggcagcg agagaccccu gacagacacc      900

gaacgcgcua cgcucaugga cgagccuuac cggaaaucua agcugacaua ugcccaggcg      960

agaaaacugc ucgggcugga ggauaccgcu uucuuuaagg gacuucgcua cggcaaggac     1020

aaugccgaag ccuccacacu gauggagaug aaggcuuauc augccauuag ccgggcucuc     1080

gaaaaagagg ggcugaaaga uaagaaaucu ccccugaacc uuuccccgga acuccaggac     1140

gagaucggaa ccgccuucag ccuguuuaag accgaugagg acaucacagg cagacugaag     1200

gaucgcauuc agccugaaau ccucgaggcg cugcuuaaac acauuucuuu cgacaaguuc     1260

guucaaaucu cccugaaagc ccuccggaga auugugcccc ugauggaaca ggggaagcgg     1320

uacgaugagg cuugcgccga gaucuacgga gaccauuaug gcaaaaagaa uacggaagag     1380

aagaucuacc ugccucccau ucccgcugau gaaauccgca acccugucgu gcuuagagcc     1440

cucagccagg cgcggaaagu gauuaacggc gucguuagac gcuauggguc cccggcccgg     1500

auccacauug agaccgcuag agaaguggga aagucuuuua aagaccgcaa ggagaucgag     1560

aaaagacaag aagagaaucg gaaggaccgc gaaaaggccg cugccaaauu cagagaguac     1620

uuucccaacu ucgugggcga accuaagagc aaagauaucc ugaagcugcg gcucuaugag     1680

cagcagcacg ggaaaugucu guacuccgga aaggagauua aucuuggccg ccugaacgaa     1740

aaaggguaug ucgagaucga ccaugcccuc cccuuuagca gaacauggga ugacucuuuc     1800

aauaacaagg ugcugguucu gggcuccgaa aaucagaaca agggaaauca aaccccuuac     1860

gaguacuuua acggcaaaga uaauagccgg gaauggcagg aguucaaggc ucgcguggag     1920

acaucuagau uuccccggag caaaaagcag cgcauucuuc uccagaaauu cgacgaagau     1980

ggguuuaagg agagaaaccu gaaugacacc cgguauguca acagauuccu gugccaauuu     2040

guggcggauc gcaugcggcu cacgggaaag ggcaaaaaga gaguuuucgc cuccaauggg     2100

cagaucacaa accugcuucg cggauucugg ggccugcgga aagugagagc ugaaaacgac     2160

cgccaccaug cccucgaugc cgucgugguc gcuuguucua ccguggccau gcagcagaag     2220

auuacccggu uuguuagaua caaagagaug aaugcguucg acgggaagac aaucgauaag     2280

gagaccggcg aagugcugca ccagaaaaca cauuuuccgc agccuuggga guucuuugcu     2340

caggaaguca ugauucgcgu guucggaaag cccgacggca aaccugaguu ugaagaggcc     2400

gacacgcccg agaagcugcg gacccuucuc gccgaaaaac ugagcuccag accugaggcu     2460

guucacgaau augugacacc ccuguucguc agccgggccc cgaaccgcaa gaugucuggg     2520

cagggacaca uggagaccgu gaaguccgcg aaaagacucg augaaggcgu gagcguucug     2580

cgggucccuc uuacacaacu gaagcucaaa gaccuggaga agauggugaa ucgcgagaga     2640

gaacccaaac uguacgaggc ucuuaaggcc cggcuggaag cccauaaaga ugaccccgcu     2700

aaggccuuug cugagccuuu cuauaaguac gauaaagccg ggaacagaac ccaacaggug     2760

aaggcggucc gcguggaaca aguucagaaa acgggagugu ggguccggaa ucacaacggc     2820

aucgccgaca augcuaccau ggugagaguu gauguguuug agaaggggga caaauauuac     2880

cucgucccga ucuacucuug gcaaguggcc aagggcauuc ugcccgaucg cgcuguugug     2940

cagggaaagg acgaggaaga uuggcagcuc aucgacgauu ccuucaacuu uaaauucagc     3000

cugcauccua augaccuugu cgaggugauu acaaagaaag cccggauguu cggcuauuuu     3060

gccuccugcc acagagggac cggaaacauc aauauucgca uccacgaccu ggaucauaag     3120

aucggcaaaa acgggauucu cgaaggaauc ggcgugaaga cagcucuguc uuuccagaag     3180

uaccagauug acgagcuggg ggaggaaauc cggcccugua gacuuaaaaa gcgcccuccc     3240

gucagauga                                                             3249


<210>  3872
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3872
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagacgaga acccgauuug ccugaucgau      120

cuuggcguua gaguguuuga acgggcugag gugcccaaaa ccggggacuc ccuggccaug      180

gcccgcagac ucgcucgguc uguccgcaga cugacacggc gcagagccca ccggcuucug      240

cgcgcuagac ggcuccugaa gcgcgaagga gugcugcagg ccgcggauuu cgacgagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuua gagccgcugc ccuggaucgg      360

aagcucaccc cucuggagug guccgcuguu cuucugcauc ucauuaaaca ccgcggguau      420

cugucucaga gaaagaauga aggagagaca gccgacaagg aacugggcgc gcuccugaaa      480

ggcguggccg auaacgcuca ugcccuucag accggggacu uucggacacc cgcugagcug      540

gcccucaaua aguucgagaa agaaagcgga cacaucagaa accagcgcgg cgauuacucc      600

cauaccuuuu cucggaagga ccugcaagcc gagcuuauuc uccuguucga aaaacagaag      660

gaguuuggga auccucacgu cagcggaggc cugaaggaag ggaucgagac acugcucaug      720

acccagagac cggcucuguc cggagaugcg gugcagaaaa ugcuuggcca cuguacguuc      780

gagcccgccg aaccuaaggc ugccaaaaac accuauacag ccgagcgcuu uauuuggcug      840

accaagcuca auaaccugcg gauccuugaa caaggcagcg agagaccccu gacagacacc      900

gaacgcgcua cgcucaugga cgagccuuac cggaaaucua agcugacaua ugcccaggcg      960

agaaaacugc ucgggcugga ggauaccgcu uucuuuaagg gacuucgcua cggcaaggac     1020

aaugccgaag ccuccacacu gauggagaug aaggcuuauc augccauuag ccgggcucuc     1080

gaaaaagagg ggcugaaaga uaagaaaucu ccccugaacc uuuccccgga acuccaggac     1140

gagaucggaa ccgccuucag ccuguuuaag accgaugagg acaucacagg cagacugaag     1200

gaucgcauuc agccugaaau ccucgaggcg cugcuuaaac acauuucuuu cgacaaguuc     1260

guucaaaucu cccugaaagc ccuccggaga auugugcccc ugauggaaca ggggaagcgg     1320

uacgaugagg cuugcgccga gaucuacgga gaccauuaug gcaaaaagaa uacggaagag     1380

aagaucuacc ugccucccau ucccgcugau gaaauccgca acccugucgu gcuuagagcc     1440

cucagccagg cgcggaaagu gauuaacggc gucguuagac gcuauggguc cccggcccgg     1500

auccacauug agaccgcuag agaaguggga aagucuuuua aagaccgcaa ggagaucgag     1560

aaaagacaag aagagaaucg gaaggaccgc gaaaaggccg cugccaaauu cagagaguac     1620

uuucccaacu ucgugggcga accuaagagc aaagauaucc ugaagcugcg gcucuaugag     1680

cagcagcacg ggaaaugucu guacuccgga aaggagauua aucuuggccg ccugaacgaa     1740

aaaggguaug ucgagaucga ccaugcccuc cccuuuagca gaacauggga ugacucuuuc     1800

aauaacaagg ugcugguucu gggcuccgaa aaucagaaca agggaaauca aaccccuuac     1860

gaguacuuua acggcaaaga uaauagccgg gaauggcagg aguucaaggc ucgcguggag     1920

acaucuagau uuccccggag caaaaagcag cgcauucuuc uccagaaauu cgacgaagau     1980

ggguuuaagg agagaaaccu gaaugacacc cgguauguca acagauuccu gugccaauuu     2040

guggcggauc gcaugcggcu cacgggaaag ggcaaaaaga gaguuuucgc cuccaauggg     2100

cagaucacaa accugcuucg cggauucugg ggccugcgga aagugagagc ugaaaacgac     2160

cgccaccaug cccucgaugc cgucgugguc gcuuguucua ccguggccau gcagcagaag     2220

auuacccggu uuguuagaua caaagagaug aaugcguucg acgggaagac aaucgauaag     2280

gagaccggcg aagugcugca ccagaaaaca cauuuuccgc agccuuggga guucuuugcu     2340

caggaaguca ugauucgcgu guucggaaag cccgacggca aaccugaguu ugaagaggcc     2400

gacacgcccg agaagcugcg gacccuucuc gccgaaaaac ugagcuccag accugaggcu     2460

guucacgaau augugacacc ccuguucguc agccgggccc cgaaccgcaa gaugucuggg     2520

cagggacaca uggagaccgu gaaguccgcg aaaagacucg augaaggcgu gagcguucug     2580

cgggucccuc uuacacaacu gaagcucaaa gaccuggaga agauggugaa ucgcgagaga     2640

gaacccaaac uguacgaggc ucuuaaggcc cggcuggaag cccauaaaga ugaccccgcu     2700

aaggccuuug cugagccuuu cuauaaguac gauaaagccg ggaacagaac ccaacaggug     2760

aaggcggucc gcguggaaca aguucagaaa acgggagugu ggguccggaa ucacaacggc     2820

aucgccgaca augcuaccau ggugagaguu gauguguuug agaaggggga caaauauuac     2880

cucgucccga ucuacucuug gcaaguggcc aagggcauuc ugcccgaucg cgcuguugug     2940

cagggaaagg acgaggaaga uuggcagcuc aucgacgauu ccuucaacuu uaaauucagc     3000

cugcauccua augaccuugu cgaggugauu acaaagaaag cccggauguu cggcuauuuu     3060

gccuccugcc acagagggac cggaaacauc aauauucgca uccacgaccu ggaucauaag     3120

aucggcaaaa acgggauucu cgaaggaauc ggcgugaaga cagcucuguc uuuccagaag     3180

uaccagauug acgagcuggg gaaagagauc cggcccugua gacuuaagaa acgcccuccc     3240

gucagauga                                                             3249


<210>  3873
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3873
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagacgaga acccgauuug ccugaucgau      120

cuuggcguua gaguguuuga acgggcugag gugcccaaaa ccggggacuc ccuggccgcc      180

gcucgcagac ucgcccgguc uguccgcaga cugacacggc gcagagcuca ccggcuucug      240

cgcgccagac ggcuccugaa gcgcgaagga gugcugcagg cggccgauuu cgacgagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuua gagcugccgc ucuggaucgg      360

aagcucaccc cucuggagug guccgccguu cuucugcauc ucauuaaaca ccgcggguau      420

cugucucaga gaaagaauga aggagagaca gcggacaagg aacugggcgc ccuccugaaa      480

ggcguggcug auaacgccca ugcucugcag accggggacu uucggacacc cgccgagcug      540

gcccucaaua aguucgagaa agaaagcgga cacaucagaa accagcgcgg cgauuacucc      600

cauaccuuuu cucggaagga ccugcaagcu gagcuuauuc uucucuucga aaaacagaag      660

gaguuuggga auccucacgu cagcggaggc cugaaggaag ggaucgagac acugcucaug      720

acccagagac cggcgcuguc cggagaugcc gugcagaaaa ugcuuggcca cuguacguuc      780

gagcccgcug aaccuaaggc cgccaaaaac accuauacag cugagcgcuu uauuuggcug      840

accaagcuca auaaccugcg gauccuugaa caaggcagcg agagaccccu gacagacacc      900

gaacgcgcca cgcucaugga cgagccuuac cggaaaucua agcugacaua ugcgcaggcu      960

agaaaacugc ucgggcugga ggauaccgcc uucuuuaagg gacuucgcua cggcaaggac     1020

aaugccgagg cuuccacacu gauggaaaug aaagccuauc augcuauuag ccgggcccuc     1080

gaaaaggagg ggcugaaaga uaagaaaucu ccccugaacc uuuccccgga acuccaggac     1140

gagaucggaa ccgcguucag ccuguuuaag accgaugagg acaucacagg cagacugaag     1200

gaucgcguuc agccugaaau ucucgaggcc cugcuuaaac acaucucuuu cgacaaguuc     1260

gugcaaauuu cccugaaagc ucuccggaga aucguccccc ugauggaaca ggggaagcgg     1320

uacgaugagg ccugcgcuga gauuuacgga gaccauuaug gcaaaaagaa uacggaagag     1380

aagaucuacc ugccucccau ccccgccgau gaaauucgca acccuguggu gcuuagagcg     1440

cucagccagg cccggaaagu caucaacggc guugugagac gcuauggguc cccggcucgg     1500

auucacaucg agaccgccag agaaguggga aagucuuuua aagaccgcaa ggagauugag     1560

aaaagacaag aagagaaucg gaaggaccgc gaaaaggcug ccgccaaauu cagagaguac     1620

uuucccaacu ucgucggcga accuaagagc aaagauaucc ugaagcugcg gcucuaugag     1680

cagcagcacg ggaaaugucu guacuccgga aaggagauca aucuuggccg ccugaacgaa     1740

aaaggguaug uggagauuga ccaugcucuc cccuuuagca gaacauggga ugacucuuuc     1800

aauaacaagg uucuggugcu gggcuccgaa aaucagaaca agggaaauca aaccccuuac     1860

gaguacuuua acggcaaaga uaauagccgg gaauggcagg aguucaaggc gcgcgucgag     1920

acaucuagau uuccccggag caaaaagcag cgcauccuuc uccagaaauu cgacgaagau     1980

ggguuuaagg agagaaaccu gaaugacacc cgguauguga acagauuccu gugccaauuu     2040

guugccgauc gcaugcggcu cacgggaaag ggcaaaaaga gaguguucgc uuccaauggg     2100

cagauuacaa accugcuucg cggauucugg ggccugcgga aagucagagc cgaaaacgac     2160

cgccaccaug cccucgaugc uguggucgug gccuguucua ccguugcgau gcagcagaag     2220

aucacccggu uugugagaua caaagagaug aaugcuuucg acgggaagac aauugauaag     2280

gagaccggcg aaguccugca ccagaaaaca cauuuuccgc aaccuuggga guucuuugcc     2340

caggaaguga ugauccgcgu uuucggaaag cccgacggca aaccugaguu ugaagaggcc     2400

gacacgcccg agaagcugcg gacccuucuc gcugaaaaac ugagcuccag accugaggcc     2460

gugcacgaau augucacacc ccuguucgug agccgggcgc cgaaccgcaa gaugucuggg     2520

cagggacaca uggagaccgu gaaguccgcu aaaagacucg augaaggcgu uagcguccug     2580

cgggugccuc uuacacaacu gaagcucaaa gaccuggaga agauggugaa ucgcgagaga     2640

gaacccaaac uguacgaggc ccuuaaggcc cggcuggaag cucauaaaga ugaccccgcc     2700

aaggcuuuug ccgagccuuu cuauaaguac gauaaagcgg ggaacagaac ccagcagguc     2760

aaggccgugc gcguugaaca agugcagaaa acgggagucu gggugcggaa ucacaacggc     2820

auugcugaca augccaccau gguuagagug gaugucuuug agaaggggga caaauauuac     2880

cucgugccga ucuacucuug gcagguugcu aagggcaucc ugcccgaucg cgccgugguc     2940

cagggaaagg acgaggaaga uuggcaacuc auugacgauu ccuucaacuu uaaauucagc     3000

cugcauccua augaccuugu ggaggugauc acaaagaaag cccggauguu cggcuauuuu     3060

gcuuccugcc acagagggac cggaaacauu aauauccgca uucacgaccu ggaucauaag     3120

aucggcaaaa acgggauccu cgaaggaauu ggcgucaaga cagcgcuguc uuuccagaag     3180

uaccagaucg acgagcuggg gaaagagauu cggcccugua gacuuaagaa acgcccuccc     3240

guuagauga                                                             3249


<210>  3874
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3874
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagaggaaa acccgauuag acugaucgac      120

cuuggcguuc ggguguuuga gcgcgcugaa gugcccaaaa ccggggauuc ccuggccaug      180

gccagacggc ucgcucgcuc ugucagacgg cugacacgca gacgggccca ccgccuucug      240

agaacgcggc gccuccugaa gagagaggga gugcugcagg cugccaauuu cgacgagaac      300

ggccucauca aaagccugcc uaauacccuu uggcaacugc gggcggccgc ucucgaucgc      360

aagcugacac cccuugaaug guccgccguu cugcuccauc ugauuaaaca cagaggguau      420

cugucucagc ggaagaacga gggagaaacc gcugacaagg agcucggcgc ccugcuuaaa      480

ggcguggcgg ggaaugccca ugcucugcag acaggagauu uuagaacccc ugccgagcuc      540

gcucugaaca aguucgaaaa agagagcggc cacauccgca aucagcgggg agacuacucc      600

cauacauuuu cuagaaagga ucuucaagcc gaacugaacc uccuguucga gaaacagaag      660

gaauuuggaa auccccacau uagcgacggc cugaaggagg ggaucgagac ccuccugaug      720

acgcagcgcc cugcccuuuc cggagaugcu guccagaaaa ugcugggcca cugcaccuuc      780

gaaccgacag agcccaaggc ggccaaaaac accuauacaa ccgaacgguu uauuuggcuc      840

acgaagcuga auaaccuuag aauccuggag caaggcagcg aacgcccucu cacagacacc      900

gagcgggcua cacugaugga ugagcccuac agaaaaucua agcugaccua ugcccaggcc      960

cgcaaacucc uggaacuuga cgacauugcu uucuuuaagg ggcugcggua cggaaaggau     1020

aaugccgagg cguccacccu cauggaaaug aaggcuuauc augccaucag cagagcccug     1080

gagaaggaag gccugaaaga caagaaaucu ccucuuaacc ucucccccga gcugcaggau     1140

gagaucggga cagcuuucag ccuguuuaag acggacgaag auauuaccgg acgccucaag     1200

gaccggaucc agccggagau ucuggaagcc cuucugaaac acaucaacuu cgauaaguuc     1260

gugcaaauuu cucucaaagc ucugagacgg aucguuccuc ugauggagca gggcaagcgc     1320

uacgacgagg ccugugcgga aaucuacggg gaucauuaug gaaagaaaaa uacagaggaa     1380

aagauugacc uucccccuau ccccgccgau gagauucaca accccguggu ccucagagcu     1440

cugucccagg cccggaaagu gaucaauggc guggucagac gcuacggcag cccugcucgg     1500

auucacaucg aaaccgccag agagguuggg aaguccuuua aagaccgcaa ggagaucgaa     1560

aaacgccaag aggaaaaccg gaaggaccgc gagaaggcgg ccgcuaaauu cagagaauau     1620

uuuccgaauu ucgugggaga gcccaagucu aaagauauuc ugaagcuccg gcuguacgag     1680

cagcagcaug gcaaaugccu uuauagcggg aaggaaauca accugggacg ccucaaugag     1740

aaaggcuacg uggaaauuga ccacgcccug ccuuuuucca gaacauggga ugacagcuuc     1800

aacaauaagg uccuggugcu ugggucugag aaccagaaua agggcaacca aacccccuau     1860

gaauacuuua auggaaaaga uaacucccgg gaguggcagg aguucaaggc ucgcguugaa     1920

acgagcagau uuccucgguc uaaaaagcag cgcauccucc ugcagaaauu cgacgaggau     1980

ggcuuuaagg aaagaaaucu gaacgacaca cgguacguga acagauuccu cugucaauuu     2040

gucgccgauc gcaugcggcu gaccgggaag ggaaagaaaa gaguguucgc cagcaauggc     2100

cagauuacca accuucugcg cggguucugg ggacuccgga aaguuagagc ugagaaugac     2160

cgccaucacg cgcuggaugc cguggucgug gcuugcucca cagucgccau gcagcagaag     2220

aucacccggu uugugagaua uaaagagaug aacgccuucg acggcaagac aauugauaag     2280

gaaacggggg agguucugca ucagaaaacc cacuuucccc aaccguggga guucuuugcu     2340

caggaaguga ugauccgcgu cuucggcaag ccugacggaa aacccgaauu ugaggaggcc     2400

gacacaccug aaaaacuucg gacccuccug acagagaaac ugucuagcag acccgaagcg     2460

gugcacgagu acguuacccc ucucuucgug ucccgggcuc ccaaucgcaa gaugagcggc     2520

caggggcaua uggaaacggu caagucugcc aaaagacugg augagggaau cuccgugcuu     2580

cgggugccgc ugacccaacu caagcugaaa gaccuggaga agaugguuaa ccgcgaaaga     2640

gagccuaaac uuuaugaagc ccugaaggcu cggcucgagg cccacaaaga ugaccccgcg     2700

aaggcuuuug ccgaacccuu cuacaaguau gauaaagccg gcaauagaac acagcagguc     2760

aaggcuaugc gcguggagca agugcagaaa accggggucu gggugcggaa ccauaaugga     2820

auugccgaca acgcuacaau gguuagagug gaugucuuug agaagggcga caaauacuau     2880

cuggugccua ucuacagcug gcagguugcc aaggggauuc ucccggaucg cgcggugguc     2940

cagggcaagg acgaagagga uuggcaacug aucgacgauu cuuucaauuu uaaauucucc     3000

cuucacccca acgaccuggu ggaaguuauu accaagaaag cccggauguu cggauacuuu     3060

gcuagcuguc acagaggcac ggggaauauc aacauccgca uucaugaccu cgaucacaag     3120

aucgggaaaa auggcauucu ggaggggauc ggagugaaga cagcccuguc cuuccagaag     3180

uaucagauug acgagcuugg caaagaaauc cggccuugca gacugaagaa aagacccccu     3240

gucagauga                                                             3249


<210>  3875
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3875
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagacgaga acccgauuug ccugaucgau      120

cuuggcguua gaguguuuga acgggcugag gugcccaaaa ccggggacuc ccuggccaug      180

gcccgcagac ucgcucgguc uguccgcaga cugacacggc gcagagccca ccggcuucug      240

cgcgcuagac ggcuccugaa gcgcgaagga gugcugcagg ccgcggauuu cgacgagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuua gagccgcugc ccuggaucgg      360

aagcucaccc cucuggagug guccgcuguu cuucugcauc ucauuaaaca ccgcggguau      420

cugucucaga gaaagaauga aggagagaca gccgacaagg aacugggcgc gcuccugaaa      480

ggcguggccg auaacgcuca ugcccuucag accggggacu uucggacacc cgcugagcug      540

gcccucaaua aguucgagaa agaaagcgga cacaucagaa accagcgcgg cgauuacucc      600

cauaccuuuu cucggaagga ccugcaagcc gagcuuaauc uccuguucga aaaacagaag      660

gaguuuggga acccucacau uagcgaugga cugaaggaag gcaucgagac acugcucaug      720

acccagagac cggcucuguc cggggacgcg guccagaaaa ugcuuggaca cuguacguuc      780

gagcccgccg aaccuaaggc ugccaaaaau accuauacag ccgagcgcuu uauuuggcug      840

accaagcuca acaaucugcg gauccuugaa caaggcagcg agagaccccu gacagacacc      900

gaacgcacga cacucauuga ugagccuuac cggaaaucua agcugaccua ugcucaggcc      960

agaaaacugc ucgagcugga caacacagcg uucuuuaagg gccuucgcua cgggaaggau     1020

aaugcugaag ccuccacccu gauggagaug aaagccuauc augcuaucag ccgggcccuc     1080

gaaaaggagg gacugaaaga caagaaaucu ccccugaacc uuuccccgga acuccaggau     1140

gagaucggca ccgcuuucag ccuguuuaag acagacgagg auauuacggg gagacugaag     1200

gaccgcgugc agccugaaau ccucgaggcc cugcuuaaac acauuucuuu cgauaaguuc     1260

guucaaaucu cccugaaagc gcuccggaga auugugcccc ugauggaaca gggaaagcgg     1320

uacgacgagg ccugcgcuga gaucuacggc gaucauuaug ggaaaaagaa caccgaagag     1380

aagaucgacc ugccucccau ucccgccgac gaaauccgca auccugucgu gcuuagagcu     1440

cucagccagg cccggaaagu gauuaacgga gucguuagac gcuacggcuc cccggcgcgg     1500

auccacauug agacagccag agaagugggc aagucuuuua aagaucgcaa ggagaucgag     1560

aaaagacaag aagagaaucg gaaggaccgc gaaaaggcug ccaccaaauu cagagaguau     1620

uuucccaacu ucguggggga accuaagagc aaagauaucc ugaagcugcg gcucuacgag     1680

cagcagcacg gaaaaugucu guauuccggc aaggagauua aucuugggcg ccugaacgaa     1740

aaaggauacg ucgagaucga ccaugcucuc cccuuuagca gaacauggga ugacucuuuc     1800

aauaacaagg ugcugguucu gggcuccgaa aaucagaaca aggggaauca aaccccuuau     1860

gaguacuuua acggcaaaga uaauagccgg gaauggcagg aguucaaggc ccgcguggag     1920

acgucuagau uuccccggag caaaaagcag cgcauucuuc uccagaaauu cgacgaagag     1980

ggauuuaagg aaagaaaccu gaaugauaca cgguacguca acagauuccu gugccaauuu     2040

guggccgacc acaugcuccu gaccggcaag gggaaacgcc ggguuuucgc uuccaacgga     2100

cagaucacca aucuucugag aggcuucugg gggcuccgca aggugcgggc ggagaacgau     2160

agacaucacg cccuggacgc ugucgugguc gccuguucua caguggccau gcagcagaaa     2220

auuacccgcu uuguucggua uaaggagaug aaugcuuucg auggaaaaac aaucgacaag     2280

gaaacgggcg aggugcugca ucaaaagacc cacuuuccgc agccuuggga guucuuugcc     2340

caggaaguca ugauuagagu guucgggaaa cccgacggca agccugaauu ugaggaggcg     2400

gauacacccg aaaaacuucg cacccuccug gcugagaagc ugagcucccg gccugaagcc     2460

guucacgagu acgugacacc ccucuucguc agcagagccc cgaaccggaa aaugucugga     2520

cagggccaua uggaaaccgu gaaguccgcu aagcgccugg acgagggggu gagcguucuu     2580

agagucccuc ugacgcaacu caaacugaag gaucuggaga aaauggugaa ucgggaacgc     2640

gagcccaaac uuuaugaagc ccugaaagcg agacucgagg cucacaagga cgaucccgcc     2700

aaagccuuug cugaaccuuu cuacaaguau gacaaggccg gaaaccggac ccaacaggug     2760

aaagcuguca gaguggagca gguucagaag acaggcgugu ggguccgcaa ucauaacggg     2820

aucgccgaua augcgaccau ggugcggguu gacguguuug agaaaggaga uaaguacuau     2880

cuggucccga ucuacucuug gcaaguggcc aaaggcauuc uccccgacag agcuguugug     2940

caggggaagg augaagagga cuggcagcug aucgaugacu ccuucaacuu uaaguucagc     3000

cuucacccua augaccuggu cgaagugauu acaaagaagg cccgcauguu cggcuacuuu     3060

gcuuccugcc accggggaac cggcaacauc aauauuagaa uccaugaucu cgaccacaaa     3120

aucgggaaga acggcauucu ggagggaauc ggggugaaaa cggcccuguc uuuccagaag     3180

uaucagauug augagcuugg aaaggaaauc cgccccuguc ggcugaaaaa gagaccuccc     3240

guccgcuga                                                             3249


<210>  3876
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3876
auggccgcuu ucaagcccaa cccuaugaau uacauccugg gccucgacau ugggaucgcc       60

agcgugggau gggcgauugu cgagaucgau gaagaggaaa acccgauuag acugaucgac      120

cuuggcguuc ggguguuuga gcgcgcugaa gugcccaaaa ccggggauuc ccuggccgcc      180

gcuagacggc ucgcccgcuc ugucagacgg cugacacgca gacgggcuca ccgccuucug      240

agagcccggc gccuccugaa gagagaggga gugcugcagg cggccgacuu cgaugagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuuc gggcugccgc ucuggaccgc      360

aagcucaccc cucuggaaug guccgccguu cuucugcauc ucauuaaaca cagaggguau      420

cugucucagc ggaagaauga gggagaaaca gcggauaagg agcugggcgc ccuccugaaa      480

ggcguggcug acaacaccca ugcccuucag acaggggauu uuagaacccc cgcugagcug      540

gcccucaaua aguucgaaaa agagagcgga cacauccgca accagcgggg cgacuacucc      600

cauacauuua auagaaagga ucugcaagcc gaacuuaacc uccuguucga gaaacagaag      660

gaauuuggga auccucacgu cucugacgga cugaaggagg gcauugagac ccugcucaug      720

acgcagcgcc cggcucugag cggggaugcg gugcagaaaa ugcuuggaca cugcaccuuc      780

gaacccacag agccuaaggc cgcuaaaaac accuauacag ccgaacgguu uguuuggcug      840

accaagcuca auaaccugag aauccuugag caaggcuccg aacgcccccu gacggacaca      900

gagcgggcca cccucaugga cgagccuuac agaaaaagca agcugacaua ugcucaggcc      960

cgcaaacugc ucgaucugga cgauaccgcg uucuuuaagg gccuucggua cgggaaggac     1020

aaugcugaag ccucuacccu gauggagaug aaagccuauc augcuauuuc cagagcccuc     1080

gaaaaggagg gacugaaaga uaagaaaagc ccccugaacc uuucuccgga acuccaggac     1140

gagaucggca cagcuuucuc ccuguuuaag acggaugagg acauuaccgg gcgccugaag     1200

gaucgggugc agccugaaau ccucgaggcc cugcuuaaac acaucagcuu cgacaaguuc     1260

guccaaauuu cucugaaagc gcucagacgg aucgugcccc ugauggaaca gggaaaccgc     1320

uacgaugagg ccuguacaga gauuuacggc gaccauuaug ggaagaaaaa uaccgaagag     1380

aagaucuacc ugccucccau ucccgcugac gaaaucagaa acccuguggu ccuucgggcc     1440

cucucccagg cuagaaaggu uaucaaugga guggugcgcc gguauggcag cccggcccgc     1500

auucacaucg agacagcgag agaagucggc aaauccuuua aggauagaaa agagauugag     1560

aagcggcaag aagagaaccg caaagacaga gaaaagucug ccgcuaaguu ccgggaguac     1620

uuucccaauu ucguggggga accuaaaagc aaggauaucc ugaaacugcg ccucuaugag     1680

cagcagcacg gaaagugccu guacuccggc aaagagauua accuugggag acugaaugaa     1740

aagggauaug uugagaucga ccaugcccuc cccuuuagcc ggaccuggga ugacucuuuc     1800

aacaauaaag ugcuggcucu gggcuccgaa aaccagaaua aggggaacca aacgccuuac     1860

gaguacuuua auggcaagga uaacagccgc gaauggcagg aguucaaagc cagagucgag     1920

acaucucggu uuccccgcag caagaaacag agaauccuuc uccagaaguu cgacgaagau     1980

ggauuuaaag agcggaaucu gaacgacacc agauauauua accgcuuccu gugucaauuu     2040

guggccgauc acaugcuccu gaccggcaag gggaagcgga gaguuuucgc uuccaaugga     2100

cagaucacaa accuucugcg cggcuucugg gggcuccgga aagugagagc ggaaaaugac     2160

cgccaucacg cccuggaugc ugucgugguc gccugcucua ccauugccau gcagcagaag     2220

aucacacggu uugugagaua caaagagaug aacgcuuucg acggaaagac gauugacaaa     2280

gagaccggcg aaguucugca ucaaaaggcc cacuuuccgc agccuuggga guucuuugcg     2340

caggaaguga ugauccgcgu cuucgggaag cccgauggca aaccugaguu ugaagaggcu     2400

gacacacccg agaaacuucg gacccuccug gccgaaaaac ugagcuccag accugaggcc     2460

gugcacaagu auguuacacc ccucuucauu agccgggcuc cgaaucgcaa aaugucugga     2520

cagggccaua uggaaaccgu gaaguccgcc aagagacugg augaggggau cagcguccuu     2580

cgggugccuc ugacgcaacu caaacugaag gaccuggaaa agauggugaa ccgcgagaga     2640

gagcccaaac uuuacgaagc gcugaaagcu cggcucgagg cccacaagga ugaccccgcc     2700

aaggcuuuug ccgaaccuuu cuauaaguac gauaaggcug gaaauagaac ccagcagguu     2760

aaagccgucc gcguggagca agugcagaag acaggcgucu gggugcauaa ccacaauggg     2820

aucgcggaca acgccaccau uguucgggug gaugucuuug aaaaaggagg caaguauuac     2880

cuggugccga ucuacucuug gcagguugcu aaagggauuc uccccgacag agccgugguc     2940

cagggcaagg augaggagga cuggacagug auggaugacu ccuucgaauu uaaguucguu     3000

cuguaugcua augaccuuau caaacugacc gccaagaaaa acgaguuccu cggauacuuu     3060

gugagccuga aucgcgccac gggcgcuauu gauauccgga cacacgacac cgauuccaca     3120

aaggggaaaa acggaaucuu ccaaucuguc ggcgugaaga ccgcgcugag cuuucagaag     3180

uaucagauug acgaacuugg gaaagagauc agaccuuguc gccugaagaa acggcccccu     3240

gugagauga                                                             3249


<210>  3877
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3877
auggccgcuu ucaagcccaa cagcaucaau uacauucugg gccucgacau cgggauugcc       60

uccgugggau gggcgauggu cgagaucgau aaagaagaga acccuauuag acugaucgac      120

cuuggcguuc ggguguuuga acgcgcugag gugccgaaga ccggggauuc ucuggccgcc      180

gcuagacggc ucgcccgcag cgucagacgg cugacacgca gacgggcuca ccgccuucug      240

agaacgcggc gccuccugaa aagagaagga gugcugcagg ccgcgaauuu cgacaagaac      300

ggccucauca aaucccugcc caauacccuu uggcaacugc gggccgcugc ccucgaucgc      360

aagcugacac cucuugagug gucugcuguu cugcuccauc ugauuaagca cagaggguau      420

cugagccagc ggaaaaacga gggagaaacc gccgacaagg agcucggcgc gcugcuuaaa      480

ggcguggccg ggaaugcuca ugcccugcag acaggagauu uuagaacccc cgcugaacuc      540

gcccugaaca aguucgagaa agaguccggc cacauccgca aucagagagg ggacuacucu      600

cauacauuua gcagaaagga ucuucaagcc gaacugaacc uccuguucga gaagcagaaa      660

gaauuuggaa auccucacau uuccggcggg cugaaggagg gaaucgaaac ccuccugaug      720

acgcagcgcc ccgcucuuag cggcgacgcg guccagaaaa ugcugggcca cugcaccuuc      780

gagccuacag agccgaaggc cgcuaaaaac accuauacaa ccgaacgguu uauuuggcuc      840

acgaagcuga auaaccuuag aauccuggag caagggucug aacgcccccu cacagauacc      900

gagcgggcca cacugaugga cgaaccuuac agaaaaucca agcugaccua ugcccaggcu      960

cgcaagcucc ugagccuuga ggauaccgcc uucuuuaaag gacugcggua cggcaaggac     1020

aaugcggagg cuucuacacu cauggaaaug aaagccuauc auacgauuuc cagagcccug     1080

gagaaggaag ggcugaaaga caaaaagagc ccccuuaacc ucucuccuga gcugcaggau     1140

gaaaucggaa ccgcuuucuc ccuguuuaaa acagacgagg auaucaccgg ccgccucaag     1200

gaccggauuc agcccgagau ccuggaagcc cuucugaaac acauuagcuu cgauaaguuc     1260

gugcaaaucu cccucaaagc ucugagacgg auuguuccgc ugauggagca ggggaagcgc     1320

uacgacgaag ccugugcgga gaucuacgga gaucauuaug gcaagaaaaa cacagaggaa     1380

aagaucuacc uuccucccau uccugccgac gagaucagaa aucccguggu ccuccgggcu     1440

cugucucagg ccagaaaagu gauuaacggc gugguccgcc gguaugggag ccccgcuaga     1500

auccacauug aaaccgcccg cgagguugga aaguccuuua aagauagaaa ggaaaucgag     1560

aagcggcaag aggaaaaucg caaagacaga gagaaggcgg ccgcuaaauu ccgggaauac     1620

uuuccuaacu ucgugggcga gcccaagagc aaagauaucc ugaagcuccg ccuguaugaa     1680

cagcagcacg ggaaaugccu uuacucugga aaggagauua aucuggugag acucaacgag     1740

aagggcuaug ucgaaaucga ccaugcccug cccuuuuccc ggacguggga cgauagcuuc     1800

aauaacaaag ugcugguucu ugggucugag aaucagaaca agggcaauca aacaccuuac     1860

gaauacuuua acggaaaaga caauagccgc gaguggcagg aguucaaggc uagaguggaa     1920

accucccggu uuccccgcuc uaaaaagcag cggauucucc ugcagaaguu cgaugaggac     1980

ggcuuuaaag aacggaaccu gaaugauacc agauauguca accgcuuccu cugucaauuu     2040

guggccgacc acauccugcu uacagggaag ggaaaacgga gaguuuucgc cagcaacggc     2100

cagauuacca aucugcuccg cggguucugg ggacugcgga aggugagagc ugagaacgau     2160

cgccaucacg cgcuggacgc cgucgugguc gcuugcucca caguggccau gcagcagaaa     2220

aucacgcggu uuguuagaua caaggaaaug aaugccuucg auggcaagac cauugacaaa     2280

gagacagggg aggugcuuca ucaaaagacc cacuuuccuc agcccuggga guucuuugcu     2340

caggaaguca ugauccgcgu guucggcaaa ccggauggaa agccugaauu ugaggaagcc     2400

gacacacucg agaaacugcg gacccugcuc gcggagaagc ugagcucuag acccgaagcu     2460

guucacgagu augugacgcc ucuuuucguc ucccgggccc ccaaccgcaa gaugagcggc     2520

caggggcaua uggaaaccgu gaaaucugcc aagagacugg augagggagu guccguucuc     2580

cgggucccuc ugacacaacu gaaacuuaag gaccuggaaa agauggugaa ucgcgagaga     2640

gagcccaagc ucuacgaagc ucugaaagcc cggcucgagg cgcacaagga cgauccggcu     2700

aaggccuuug ccgaaccuuu cuauaaauac gacaaggcug gcaacagaac ccaacaggug     2760

aaagccgucc gcguggagca gguucagaag acaggggugu ggguccggaa ucauaacgga     2820

aucgcugaua augccaccau ggugagaguu gacguguuug aaaaaggcga uaaguauuac     2880

cuggucccca uuuacagcug gcaaguggcg aaggggaucc uucccgaccg cgccguugug     2940

cagggcaaag augaggagga cuggcagcug auugaugacu ccuucaacuu uaaguucucu     3000

cuccacccua augaucuggu cgaagugauc acgaaaaagg cucggauguu cggauauuuu     3060

gccagcuguc acagaggcac agggaacauu aauauccgca uccaugaccu ggaucacaaa     3120

auuggaaaga acggcauccu ugaggggauu ggagugaaga ccgcucuguc cuuccagaaa     3180

uaccagaucg acgaacucgg caaggagauu cggccgugca gacugaaaaa gcgcccgccu     3240

gucagauga                                                             3249


<210>  3878
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3878
auggccgcuu ucaagcccaa cccuaucaau uacauucugg gccucgacau cgggauugcc       60

agcgugggau gggcgauggu cgagaucgau gaagaggaaa acccgauuag acugaucgac      120

cuuggcguuc ggguguuuga gcgcgcugaa gugcccaaaa ccggggauuc ccuggccaug      180

gucagacggc ucgcccgcuc ugugagacgg cugacacgca gacgggcuca ccgccuucug      240

agagcccggc gccuccugaa gagagaggga guucugcagg cugccgacuu cgaugagaau      300

ggccucauca aaagccugcc uaacacgccc uggcaacuuc gggcggccgc ucuggaccgc      360

aagcucaccc cucuggaaug guccgccgug cuucugcauc ucauuaaaca cagaggguau      420

cugucucagc ggaagaauga gggagaaaca gcugauaagg agcugggcgc ccuccugaaa      480

ggcgucgcgg acaacgccca ugcucugcag accggggauu uuagaacacc cgccgagcug      540

gcucucaaua aguucgaaaa agagagcgga cacauccgca accagcgggg cgacuacucc      600

cauaccuuuu cuagaaagga ucugcaagcc gaacuuauuc uucucuucga gaaacagaag      660

gaauuuggga auccucacau cagcggaggc cugaaggagg ggauugagac acugcucaug      720

acccagcgcc cggcccuguc cggagacgcu gugcagaaaa ugcuuggcca cugcacguuc      780

gaacccgcgg agccuaaggc cgcuaaaaac accuauacag ccgaacgguu uaucuggcug      840

accaagcuca auaaccugcg cauucuugag caaggcagcg aaagaccccu gacagauacc      900

gagcgggcca cgcucaugga cgagccuuac agaaaaucua agcugacaua ugcucaggcc      960

cgcaaacugc ucgggcugga agacaccgcg uucuuuaagg gacuucggua cggcaaggau     1020

aaugcugagg ccuccacacu gauggaaaug aaagccuauc augcuaucag cagagcccuc     1080

gagaaggaag ggcugaaaga caagaaaucu ccccugaacc uuuccccgga gcuccaggau     1140

gagaucggaa ccgcuuucag ccuguuuaag accgacgaag auauuacagg ccgccugaag     1200

gaccggaucc agccugagau ucucgaagcc cugcuuaaac acaucucuuu cgauaaguuc     1260

guucaaauuu cccugaaagc gcucagacgg aucgugcccc ugauggagca ggggaagcgc     1320

uacgacgagg ccugugcuga aaucuacgga gaucauuaug gcaaaaagaa uacggaggaa     1380

aagauuuacc ugccucccau ccccgccgac gagauuagaa acccugucgu gcuucgggcu     1440

cucagccagg ccagaaaagu gaucaacggc gucguucgcc gguauggguc cccggcgcgc     1500

auucacaucg aaaccgccag agagguggga aagucuuuua aagauagaaa ggagaucgaa     1560

aaacggcaag aggaaaaucg caaggacaga gagaaggcug ccgcuaaauu ccgggaauac     1620

uuucccaacu ucgugggcga gccuaagagc aaagacauuc ugaagcugcg ccucuaugag     1680

cagcagcacg ggaaaugccu guacuccgga aaggaaauca aucuuggcag acugaacgag     1740

aaaggguaug ucgaaauuga ucaugcccuc cccuuuagcc ggacauggga cgauucuuuc     1800

aauaacaagg ugcugguucu gggcuccgag aaucagaaca agggaaauca aaccccuuac     1860

gaauacuuua acggcaaaga caauagccgc gaguggcagg aguucaaggc cagaguggaa     1920

acaucucggu uuccccgcag caaaaagcag agaauccuuc uccagaaauu cgaugaggac     1980

ggguuuaagg aacggaaccu gaaugauacc agauauguca accgcuuccu gugucaauuu     2040

guggcugacc ggaugagacu cacgggaaag ggcaaaaagc gcguuuucgc guccaauggg     2100

cagauuacaa accugcuucg gggauucugg ggccugagaa aagugcgcgc cgagaacgau     2160

cggcaccaug cucucgacgc cgucgugguc gccugcucua ccguggcuau gcagcagaag     2220

aucaccagau uuguucgcua caaagagaug aaugccuucg augggaagac aauugacaag     2280

gaaaccggcg aggugcugca ccagaaaaca cauuuuccgc agccuuggga guucuuugcg     2340

caggaaguca ugauccgggu guucggaaag cccgauggca aaccugaauu ugaggaggcu     2400

gacacgcccg aaaagcugag aacccuucuc gccgagaaac ugagcucccg gccugaagcc     2460

guucacgagu augugacacc ccuguucguc agccgcgcuc cgaacagaaa gaugucuggg     2520

cagggacaca uggaaaccgu gaaguccgcc aaacggcucg acgagggcgu gagcguucug     2580

cgcgucccuc uuacacaacu gaagcucaaa gaucuggaga agauggugaa uagagaacgg     2640

gagcccaaac uguacgaagc gcuuaaggcu agacuggagg cccauaaaga cgaucccgcc     2700

aaggcuuuug ccgaaccuuu cuauaaguac gacaaagcug ggaaccgcac ccaacaggug     2760

aaggccgucc ggguggagca aguucagaaa acgggagugu gggucagaaa ucacaacggc     2820

aucgcggaua augccaccau ggugcgcguu gacguguuug agaaggggga uaaauauuac     2880

cucgucccga uuuacucuug gcaaguggcu aagggcaucc ugcccgaccg ggccguugug     2940

cagggaaagg augaagagga cuggcagcuc auugaugacu ccuucaacuu uaaauucagc     3000

cugcauccua augaucuugu cgaagugauc acaaagaaag cuagaauguu cggcuauuuu     3060

gccuccuguc accgcgggac cggaaacauu aauauccgga uccacgaccu ggaccauaag     3120

auuggcaaaa acgggauccu cgagggaauu ggcgugaaga cagcccuguc uuuccagaag     3180

uaccagaucg augagcuggg gaaagaaauu agacccugcc gccuuaagaa aagaccuccc     3240

guccgguga                                                             3249


<210>  3879
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3879
augagagugc ugggccucga cgccgggauc gcuagccugg gaugggcccu uauugagauc       60

gaagagucca accggggcga acugucucag gggaccauua ucggagcggg cacauggaug      120

uucgaugcuc ccgaggaaaa gacgcaagcc ggggccaaac ucaagagcga gcagcgcaga      180

accuuucggg gacagcgcag agucguucgg cgcagacggc agcgcaugaa ugaggugaga      240

cggauucugc acucccaugg ccuucugccu ucuagcgacc gcgaugcucu caaacaaccg      300

ggccuggacc ccuggagaau ccgggccgaa gcucuggauc gccuccuggg gccuguggag      360

cuugccgucg cgcugggaca caucgccaga caucggggcu ucaaguccaa cucuaaaggg      420

gcuaagacaa augaccccgc cgaugacacc agcaagauga aaagagcugu gaacgaaaca      480

cgcgagaagc ucgcccgguu uggauccgcg gccaaaaugc ugguugagga ugagagcuuc      540

gugcuuagac agaccccuac aaagaauggc gcuucugaaa uuguccgccg guuuagaaac      600

cgcgaagggg acuacucccg gagccugcuc agagaugacc uggccgcuga gaugcgcgcc      660

cuguucaccg cccaggcucg guuucagucu gcgaucgcca cggcugaucu ccaaaccgcc      720

uucacaaaag ccgcuuucuu ccagagaccc cugcaggacu ccgaaaagcu ggugggaccu      780

ugcccguuug agguugacga gaagcgcgcc cccaaacggg gcuauagcuu cgaacuguuu      840

agauuccucu cucggcugaa ucacgugacc cuucgcgaug gcaagcagga gagaacacug      900

acccgggacg aacucgcgcu ggcugccgcc gauuucgggg cugccgcuaa agucuccuuu      960

acggcccuga gaaagaaacu caagcugccu gagacaaccg uguucguggg agucaaagcg     1020

gacgaagaga gcaaacuuga uguuguggcc cgcuccggca aggcugccga ggggacagcu     1080

cggcugagau cugugauugu cgacgcccuc ggagaacugg cguggggcgc ccuucugugu     1140

agccccgaga agcucgauaa gaucgcugaa gugauuuccu uucgcagcga caucgggaga     1200

auuucugagg gacuggccca agcuggcugc aacgccccuc ugguugaugc ccucaccgcu     1260

gcggccuccg acggccgguu cgaucccuuu accggggcug gacauaucag cucuaaagcc     1320

gcccgcaaua uccugagcgg ccuuagacag gggaugacau acgacaaggc uuguugcgcc     1380

gcggacuaug aucacacggc uucccgggaa cgcggagccu ucgacguggg cgggcacggc     1440

agagaggccc ugaaacggau ucuccaggag gaacgcaucu cuagagagcu ggucggaagc     1500

ccgaccgcuc ggaaggcccu gauugaaucc aucaagcaag ugaaagcgau uguugagcgc     1560

uacggcgugc cugauagaau ccaugucgag cuugcucggg acgugggaaa gagcaucgaa     1620

gagagagaag agauuacacg cgggaucgaa aaacggaaca gacagaagga uaaacuccgc     1680

ggccuguuug agaaggaggu cgggcggccc ccucaggacg gagccagagg caaggaggaa     1740

cugcuccgcu ucgaacugug gucugagcag auggggcggu gucuuuauac cgaugacuac     1800

auuuccccca gccaacuggu ggccacagau gacgcuguuc agguggauca cauccucccc     1860

uggucuagau uugccgacga uuccuaugcu aauaaaaccc ugugcauggc caaggcgaac     1920

caggacaaaa agggccgcac gccuuacgaa ugguucaaag ccgagaagac agacaccgag     1980

ugggaugcuu uuauuguccg gguggaagcc cuggcugaca ugaagggauu caaaaagaga     2040

aauuacaaac uucggaacgc cgaggaagcc gcugcgaagu uucgcaauag aaaccucaac     2100

gauacccggu gggccugucg ccugcuggcu gaggcccuca aacagcugua uccgaagggc     2160

gaaaaagaca aggaugggaa ggagagacgg agaguuuuca gccgccccgg agcccuuaca     2220

gaccggcuga gacgcgcuug gggccuccaa uggaugaaaa aguccaccaa aggggaucgg     2280

aucccugacg auagacauca cgcccuggac gcgaucguga uugcugccac aacggagucu     2340

cugcuucagc gcgccacccg ggaaguccag gagaucgaag auaagggacu ccacuacgac     2400

cuggugaaaa augugacacc cccuuggccc ggcuucagag agcaggcugu ugaggccguc     2460

gaaaaggugu uuguggcgcg cgcugagaga cggcgcgcca gagggaaggc ccaugaugcu     2520

accauucggc acaucgccgu ccgcgaaggc gagcaaagag uguaugaacg gagaaaaguu     2580

gcugagcuga agcucgccga ccuggaccgc gugaaagaug cggagcggaa cgccagacuu     2640

auugaaaagc ugcgcaauug gaucgaggcu ggaagcccga aagacgaucc uccccucucc     2700

ccuaagggcg accccauuuu caagguccgg cuggugacaa aaucuaaggu uaacaucgcc     2760

cuggauaccg ggaauccuaa aagacccgga acgguggacc ggggcgaaau ggcucgcguc     2820

gauguguuua gaaaggccag caaaaagggg aaguacgagu auuaccuugu uccgaucuau     2880

ccucaugaca uugccaccau gaaaacaccu cccauccggg cugugcaggc guacaagccc     2940

gaagaugagu ggccggagau ggacuccagc uacgaauuuu gcuggucucu cguccccaug     3000

accuaucuuc aggugauuuc cagcaaagga gagaucuuug aaggcuacua ucgcgggaug     3060

aacagaucug ugggcgccau ucagcugucc gcucacagca auucuagcga ugucguucaa     3120

ggaaucggcg cccggacacu caccgaguuc aagaaauuua acguggaccg cuucgggaga     3180

aagcaugaag uggagcggga gcugcgcacg uggagaggag aaacauggcg gggcaaagcc     3240

uacaucuga                                                             3249


<210>  3880
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3880
augaagaaca cccuguacgg caucgggcuc gacauuggag uggccagcgu cggcugggcu       60

guuguggggc ugaauggaac aggcgagccc guggggcuuc acagacuggg aguccggauc      120

uucgauaaag ccgaacagcc uaagacgggc gagucccucg cggcuccgcg cagaauggcc      180

cggggcaugc gcagacggcu gcgcagaaaa gcccuucggc gcgcugacgu guaugcccug      240

cucgaaagau cugggcugag cacccgggag gcucuggccc auauguuuga agcgggaggc      300

cucgaggaua uuuacgcccu gcgcacaaga gcucuugacg agcccguugg gaaggccgag      360

uucucccgga uccugcucca ccuggcucaa cgcagaggau uuaaaucuaa ccggagaacc      420

gccagcgaug gcgaagacgg gcgccuucug gcggccguga augaaaaccg gagacgcaug      480

gcucagggag gcuggcggac agucggcgag augcucuaua gacaugaggc cuucgcucug      540

cacaagcgca auaaggccga ugaauaccug uccaccgugg ggcgggacau gguugccgag      600

gaggcuucuc uccuguuuca gagacagcgc gaacuuggau gcgcgugggc cacaccugaa      660

cugcaagcug aguaucucag cauucugcuu cggcagagau ccuucgauga gggccccggg      720

ggaaacagcc cuuacggcgg gaaucaggug gaaaagaugg ucggacgcug uaccuuugag      780

cccgacgaac cucgggccgc caaggcugcc uauucuuucg aguacuuuuc ccugcuccag      840

aaaaucaacc auaucagacu gguggaaaau ggcgagacgc ggccgcugac ccaacagcag      900

cgccagcaac uccugagccu ugcgcacaag acacccgaug ugucucuggc ucggauuaga      960

aaagagcucg cccugccuga aaccguccag uucaacggcg uuagaugccg cgccaaugag     1020

acacuugaag aguccgaaaa gaaggagaaa uuugcuuguc ugcccgccua ccacaagaug     1080

cggaaagcuc ucgacggggu ggugaaggga agaaucagcu cucuguccau uagccagcgc     1140

gaugccgcgg ccaccgcucu gucccucuau aaaaacgagg acacgcugag agccaaacuu     1200

acagaagcug gcuuccaagc cccugagauc gacgcgcugg ccgggcucac cggauuuucu     1260

aaauucggcc aucugagccu gaaggcuugc cggaaacuua uuccccaccu cgaacagggg     1320

cugacauacg aucaggccug uuccgcugcc ggcuaugacu ucaagggaca uggcgccggg     1380

gagcgcgcuu uuacccugcc ggcggccgcu ccugagaugg aacagaucac cagccccguc     1440

gugagacggg ccguugccca aacaauuaag guggucaaug gaaucauccg cgagauggau     1500

gcuucuccug ccugggugag aauugaacuc gcgcgggagc uguccaaaac guucggcgaa     1560

cgccaggaga uggacagaag caugcgggag aacgcugccc agaaugaacg ccuuaugcag     1620

gagcugagag auaccuuuca ccuccugucu cccacagggc aagaccuggu uaaguaccgg     1680

cuuuggaaag aacaggaugg agugugcgcc uauagccuca gacgccugga cgucgagcgg     1740

cuguucgaac ccggcuacgu ggaugucgac cacaucgugc cuuauucccu cucuuuugau     1800

gacagacgca gcaacaaggu ucuggugcuu uccagcgaga accggcagaa ggggaauaga     1860

cugccgcucc aguaccugca aggcaaacgc cgggaggacu ucauugucug gaccaacucu     1920

uccgugagag auuaccgcaa gcggcagaau cugcuuagag aaaaacucag cggagacgag     1980

gcugaaggcu uucggcagcg caaccugcag gauacacaac auauggccag auuccuguau     2040

aauuacaucu cugaccaccu cgcguuugcu caguccgagg cccuggggaa gaaacggguu     2100

uucgccguga gcggagcugu caccucccau cuucgcaaga gauggggccu gucuaaggug     2160

cgggccgaug gggaccucca ccaugcucug gaugccgcgg ugauugccug uacgacagac     2220

ggaaugauca gacgcaucag cggcuauuac gggcacauug aaggcgagua ucugcaggau     2280

gcugacggag ccggcuccca acacgcucgg accaaagaga gauuucccgc cccuuggccc     2340

cgcuuccggg augaacuuau cguuagacuc ucugagcagc cuggggaaca ucugcuggac     2400

auuaaccccg aguuuuacug cgaguaugga accgaacaca uccgcccggu cuucgugagc     2460

cggaugccua gacgcaaggu gacaggcccc gggcauaaag agaccauuaa gggagccgcu     2520

gcggccgaug aaggccuccu gacagucaga aaagcucuua cggagcugaa gcucgacaag     2580

gacggggaaa ucaaagauua cuacaugccu uccagcgaca cccugcugua ugaggcccuu     2640

aaggcccagc ugcggcgcuu cggcggagau ucuaaaaagg cuuuugccga gcccuucuac     2700

aaaccuaagg cggacggcac acccgggccg cucgugagaa aaguuaagac cacagaaaag     2760

gcuacccuga cggugccugu ccacggaggc gccgccucca augauaccau ggugcggguu     2820

gacguguuuc ucguccccgg ggauggauau uacugggugc ccguuuauac agcugacacc     2880

cugaaaccug agcuuccgaa ccgcgccgug guccagggca agcccuacag cgaauggaaa     2940

gagaugagag aagaggauuu caucuuuucu cuguauccua augaccucgu guacguggag     3000

cacaaguccg gccugaaauu cacacugcaa aacgcggaua gcacccuuga aaagacgcgg     3060

acacagaccu cugcuuuugu cuacuucguu gggggaagca uuuccacagc cagcaucacc     3120

auuagaaccc augacaaugc cuauggcauc cccucucugg gggugaagac acucaaaacg     3180

uuucacaagu accaggugga uguccuggga aacguguucc cuguucauaa agagacccgc     3240

cagaaguuuc gguga                                                      3255


<210>  3881
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3881
augaagaaca cccuguacgg caucgggcuc gacauuggag uggccagcgu cggcugggcu       60

guuguggggc ugaauggaac aggcgagccc guggggcuuc acagacuggg aguccggauc      120

uucgauaaag ccgaacagcc uaagacgggc gagucccucg cggcuccgcg cagaauggcc      180

cggggcaugc gcagacggcu gcgcagaaaa gcccuucggc gcgcugacgu guaugcccug      240

cucgaaagau cugggcugag cacccgggag gcucuggccc aaauguuuga agcgggaggc      300

cucgaggaua uuuacgcccu gcgcacaaga gcucuugacg agcccguugg gaaggccgag      360

uucucccgga uccugcucca ucuggcucag cgcagaggau uuaaaucuaa ccggagaacc      420

gccagcgaug gcgaagacgg gcgccuucug gcggccguga augaaaaccg gagacgcaug      480

gcucagggag gcuggcggac agucggcgag augcucuaua gacacgaggc cuucgcucug      540

cauaagcgca auaaggccga ugacuaccug uccaccgugg ggcgggauau gguugccgaa      600

gaggcuucuc uccuguuuca gagacaacgc gaacuuggau gcgcgugggc cacaccugag      660

cugcaggcug aauaucucag cauucugcuu cggcagagau ccuucgacga gggccccggg      720

ggagauagcc cuuacggcgg gaaccaggug gagaaaaugg ucggacgcug uaccuuugaa      780

cccgacgagc cucgggccgc caaggcugcc uauucuuucg aauacuuuuc ccugcuccaa      840

aagaucaauc acaucagacu ggcggagaac ggcgaaacgc ggccgcugac ccagccccag      900

cgccagcaac uccugagccu ugcucauaag acaccugaua ugucucuggc ccggauuaga      960

aaagagcucg cccugcccga gaccgugcag uucaauggcg ugagaugccg cgcuaacgaa     1020

acacuugagg aauccgagaa gaaggaaaaa uuugccuguc ugccugcuua ccacaagaug     1080

cggaaagccc ucgggggagu cguuaagggc agaaucagcu cucuguccau uagccagcgc     1140

gacgcggccg cuaccgcccu gucccucuau aaaaaugagg acacgcugag agcuaaacuu     1200

acagaggccg gguuccaagc gcccgaaauc gaugcccugg cuggacucac cggcuuuucu     1260

aaauucgggc accugagccu gaaggccugc cggaaacuua uuccgcaucu cgagcagggc     1320

cugacauacg accaggcuug uuccgccgcc ggauaugauu ucaagggcca cggggcugga     1380

gaacgcgcgu uuacccugcc ugccgcugcc cccgagaugg agcagaucac cagcccugug     1440

gugagacggg ccgucgcuca aacaauuaag gugguuaacg gcaucauccg cgaaauggac     1500

gccucucccg cgugggugag aauugagcuc gcucgggaac uguccaaaac guucggggag     1560

cgccaggaaa uggauagaag caugcgggag aaugccgcca agaacgagcg ccuuaugcag     1620

gaacugagag acaccuuuca ucuccugucu cccacaggac aggaucuggu caaauaccgg     1680

cuuuggaagg agcaagacgg cgugugcgcu uauagccucc agagacugga uguugaacag     1740

cuguucgagc cuggguacgu ggacgucgau cacaucgugc cguauucccu cucuuuugac     1800

gaccgccgga gcaauaaggu ccuggugcuu uccagcgaaa acagacagaa aggcaaccgc     1860

cugccccucc aauaccugca gggaaagcgg agagaggauu ucauuguuug gaccaauucu     1920

uccgugcacg acuaccgcaa acggcagaac cugcuuagag agaagcucag cgaugacgaa     1980

gccgagggcu uucgccagcg gaaucugcaa gauacacagc auauggcgag auuccuguau     2040

aacuacaucu cugaccaccu cgcuuuugcc caguccgaag cccuggggaa aaagcggguc     2100

uucgcuguga gcggagccgu uaccucccau cuucgcaaga gauggggccu gucuaaagug     2160

cgggcugaug gggaccucca ccaugcccug gaugcggccg ucguggcuug uacgacagac     2220

ggaaugauuc gcagaaucag cggcuauuac gggcacaucg agggcgaaua uuuucaagau     2280

gccgacggag cuggcuccca gcacgcccgg accaaggaga gauucccugc ccccuggccu     2340

cgcuuucggg augagcugau ugugagacuu ucugaacagc ccggggagcc gcuccuggac     2400

aucaauccug cuuucuacug cgaauaugga accgagcaua uucgccccgu uuucgucagc     2460

cggaugccua gacgcaaagu gacaggcccc ucccacaagg agaccgugaa aggggcggcc     2520

gcugccgacg aaggacugcu cacaguccgg aaggcccuga cggagcuuaa gcuggauaaa     2580

gacggcgaaa ucaaggauua cuacaugccu agcucugaca cccuccugua ugaggcucug     2640

aaagcccagc uuagacgcuu ugggggcgau ggaaagaaag cguucgcuga acccuuuuac     2700

aagccgaaag ccgacggcac accugggccc cuggugagaa agguuaagac cacagagaaa     2760

gccacccuca cggugcccgu ccauggaggc gcugccucca acgauaccau ggugcggauu     2820

gacguuuucu uuaugccugg ggauggauau uacugggugc cgcuguaugu cgcggacaca     2880

cucaagcccg agcugccuaa ucgcgcugug guugcccaca aacccuacag cgaauggcaa     2940

gagaugagag aagaggauuu cguguuuucu cuuuauccua acgaccuggu cuacguggaa     3000

cacaaguccg agcucaaauu caccgugcag aaugccgaua gcacacugga gaagacccgg     3060

acgcaggcuu cugccuuugu cuacuucguu agcggcggca ucuccggggg aacaaucacc     3120

gugcgcacac augacaacag cuauggcauu cccucucugg ggcuuaagac ccugaaaucc     3180

cucuguaagu accaggugga cguccuggga aauguguuuc cuguucacaa agaaaccaga     3240

caaaaguucc gguga                                                      3255


<210>  3882
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3882
augaagaaca cccuguacgg caucgggcuc gacauuggag uggccagcgu cggcugggcu       60

guuguggggc ugaauggaac aggcgagccc guggggcuuc acagacuggg aguccggauc      120

uucgauaaag ccgaacagcc uaagacgggc gagucccucg cggcuccgcg cagaauggcc      180

cggggcaugc gcagacggcu gcgcagaaaa gcccuucggc gcgcugacgu guaugcccug      240

cucgaaagau cugggcugag cacccgggag gaacuggcuc aaauguuuga ggccggaggc      300

cucgaggaua uuuacgcgcu gcgcacaaga gcccuugacg aacccguugg gaaggcugag      360

uucucccgga uccugcucca ucuggcccag cgcagaggau uuaaaucuaa ccggagaacc      420

gcuagcgaug gcgaagacgg gcgccuucug gccgcgguga augagaaccg gagacgcaug      480

gcccagggag gcuggcggac agucggcgag augcucuaua gacacgaggc uuucgcccug      540

cauaagcgca auaaggcuga ugacuaccug uccaccgugg ggcgggauau gguugccgaa      600

gaagccucua uucucuuuga gagacagcgc gcuaugggau gcagcugggc ggccccugaa      660

cugcaagcug aguaucuuuc cauccugcuc cggcagcgca gcuucgacga gggccccggg      720

ggaaacucuc cuuacggcgg gaaucaggug gagaaaaugg ucggaagaug uacauuugaa      780

cccgaugaac cucgggccgc caaggcugcc uauuccuucg aguacuuuag ccugcuucag      840

aaacugaacc acaucagacu cgcggaaaau ggcgagaccc ggccgcugac gcaaccccag      900

cgccagcagc ugcucucucu ggcucauaag accccugaca uuucccuugc cagaauccgg      960

aaagagcugg cccuccccga aacagugcaa uucaacggcg ugagaugccg cgcuaaugag     1020

acccuggaag agagcgaaaa gaaggagaaa uuugccuguc uuccugcuua ccacaagaug     1080

cggaaagccc uggauggggu cguuaaggga agaauuucuu cccucagcau cucccagcgc     1140

gacgcggccg cuacagcccu gucucuguau aaaaacgagg acacccucag agcuaagcug     1200

acggaagccg gcuuccaggc gcccgagauu gaugcccuug cugggcugac aggauuuagc     1260

aaauucggcc accucucccu gaaggccugc cggaagcuga ucccgcaucu ugaacaaggg     1320

cucaccuacg accaggcuug uacagccgcc ggcuaugauu ucaaaggaca cggcgcuggg     1380

gagcgcgcgu uuacccugcc ugccgcugcc cccgagaugg aacagauuac cagcccugug     1440

gugagacggg ccgucgcuca gacaaucaag gugguuaaug gaaucauucg cgagauggac     1500

gccucucccg cgugggugag aaucgaacug gcucgggagc ucuccaaaac guucggcgaa     1560

cgccaagaga uggauagaag caugcgggag aacgccgccc agaaugaacg ccugaugcag     1620

gagcuuagag acaccuuuca ucugcucucu cccacagggc aggaucuggu caaguaccgg     1680

cuguggaaag aacaagacgg agugugcgcu uauagccuua gacgccucga uguugagcgg     1740

cuguucgaac cuggcuacgu ggacgucgau cacauugugc cguauucccu gucuuuugac     1800

gacagacgca gcaacaaggu ccucgugcug uccagcgaga accggcagaa ggggaauaga     1860

cuuccccugc aguaccucca gggcaaacgc cgggaggauu ucaucguuug gaccaacucu     1920

uccgugcacg acuacagaaa gcgccaaaau cugcugcggg aaaaacuuag cggagaugag     1980

gccgaaggcu uuagacagcg gaaccuccag gacacacagc auauggcgcg cuuccuguau     2040

aauuacauuu cugaucaccu ggcuuuugcc caauccgagg cccucgggaa gaaaagaguc     2100

uucgcuguga gcggagccgu uaccucccau cugcggaagc gcuggggccu uucuaaggug     2160

agagcugacg gggaucugca ccaugcccuc gacgcggccg ucguggcuug uacgacagau     2220

ggaaugaucc ggagaaucag cggcuauuac gggcacauug aaggcgagua ucugcaggac     2280

gccgauggag cuggcuccca gcacgcccgc accaaagagc gguuuccugc ccccuggccu     2340

agauuccgcg acgaacugau cgugcggcuu ucugagcaac ccgcugaacc gcuccuggau     2400

auuaacccug aguuuuacug cgaguauggg accgaacaua ucagacccgu uuucgucagc     2460

cgcaugccuc ggagaaaggu gacaggaccc ggccacaaag agaccauuaa gggggcggcc     2520

gcugccgacg aaggacugcu cacagugcgc aaagcccuga cggagcuuaa gcuggacaag     2580

gauggcgaaa ucaaagacua cuacaugccu uccagcgaua cccuccugua ugaggcucug     2640

aaggcccagc uuagacgguu cgggggcgac ggcaaaaagg cguuugcuga gcccuucuac     2700

aaaccgaagg ccgauggaac accugggccc cugguccgca aagugaagac cacagaaaag     2760

gccacccuca cgguucccgu gcauggaggc gcugccucua augacaccau ggucagagug     2820

gauguuuuuc uggugccugg ggacggauau uacugggucc cgguguauac agcggauacc     2880

cucaaacccg agcugccuaa ccgggcuguu gugcagggca agcccuacuc cgaauggaaa     2940

gagaugcgcg aagaggacuu caucuuuagc cuuuauccua augaucuggu cuacguggag     3000

cacaagucug gccucaaauu cacacugcag aacgccgacu ccacccugga aaagacgaga     3060

acacaaacca gcgccuuugc uuacuucgug ucugggagca uuuccacagc cagcaucacc     3120

auucggaccc acgauaaugc uuauggaauc cccucucuug gcgucaagac acugaaaacg     3180

uuucauaagu accagguuga cgugcucggg aacguguucc cuguccacaa agagaccaga     3240

cagaaguuuc gcuga                                                      3255


<210>  3883
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3883
augagacugg gccucgacau cgggaccaac agcauuggau gguggcugua cggcacagaa       60

gggggacgga ucacggaggu ggucgauggc gggguucgca uuuucuccga cggaagagau      120

ccccagucua aggccagccu ugcuguggac cggcgcgccg cgagauccca acggcgcaga      180

cgggaucgcu aucugagacg gaaagcugcc cucaugaagc gcauggccca ggugggccug      240

augccugcug acccggccga ggcuaaaguc cuugaagugc ugaaucccua cgagcucaga      300

gccucuggcc uggaugggcc ucugccccuc acccacuuug gacgggcgcu guuccaucuu      360

aaccagcgca gaggcuuuaa gagcaaucgg aaaacagaca gaggggauaa cgaauccgga      420

aagaucaagg acgccaccgc ucgccuggau caggccauga uggcuaaagg cgcccggaca      480

uauggggagu uccuccacau gagacgcgcg gccgcucugg acccuaaguc uguucccagc      540

gugcggacca gacuuacagu cgcccgccgg gaugacgcug agaaagaaga ggccggauac      600

gacuuuuauc cugauagaaa gcaucugucc gaagaguucg acaaacucug ggccgcucaa      660

gcgcgccacg ccccggaugu gcugaccgag gaccugcggg augaaauugc uacgaucauc      720

uuucaucaga gaccccucaa gaccccugag guuggccugu gccuuuucac aggccagcac      780

ggggugcccg aacgcgacaa gcggauuccu agcgcccacc cccugaauca gagacggauc      840

cucauggaga ccgucaacaa ucugaaagug gccgcucaag gagaaccguc ucgcccucuu      900

acaagagagg agcgggauca gauugugcau gcccuggaca acaagaaaca caccaagucc      960

cucagcggca ugacgaugaa acugaaggcg cuggggaaag ucaucaagcu cagacccgaa     1020

cagggauuua cacuggagac cgcuaaucgc gaugccauug ccugugaccc uguucgggcu     1080

ucucuuuccc aucccgauag auucggcgcc cgcuggagca cacuggaauc ugcugggcag     1140

ugggacgugg ugcaaagaau ccgggccguc caguccgacg cggagcacgc cgaucucgug     1200

ggauggcuga uggacguuca cggccuugau cgcgaccaug cugaagagac cgccaacgcu     1260

ccccugccug agggcuacgg gagacucgga cugaccgcca caacgcggau ucuggcggcc     1320

cucgaagcug augugcugaa uuauagcgcc gcugucgccg ccugcggcug gcaccauucc     1380

gacgggcgca ccggagaggu gcuugaaucu cugccguacu acggcgagau ccucgaaaga     1440

cacguuaucc ccgggacagg cgauccuaac gacgaugacg ugaagcggua uggacgcauu     1500

accaauccca caguccauau cggccugaac cagcugagac ggcuugugaa ucgcauuauc     1560

accgucuacg ggagaccuga ugagauugug guugagcucg cucgggaccu gaaacugagc     1620

gaagaucaaa agaaagacgu gcagagagac aucaagaaaa acacggaggc ggccauccgc     1680

cggggaaaga agcucgaaga gaugggcguc cccaacacag gggcuaauag aauggugcug     1740

cgccuuuggg aggaucuggg agacgauguu augacccgga acuguccgua uaccggcaaa     1800

agaauuuccg ccgggaugcu cuuugacggc agcugcgaug uggaccacau ccugccuuac     1860

ucucgcacac uggaugacuc cuucgccaau cggacccuuu gucucaagga agcuaacaga     1920

cagaaaacag auaagacgcc cugggaggcc uggggagaaa ccgagcagug ggaagcgauu     1980

gcugccaauc ugaaaaaccu gccugccaau aaggcuuggc gcuuugcccc cgacgcgaug     2040

gagcgguucg agggcgaaaa ggauuucagc gcuagagccc ucgccgacac acaauaucug     2100

ucucggaucg cucgcgccua ccuugaugcu cuguaugacg gggacgaugg aaaaagccac     2160

gucugggugg ugccuggcag acucaccgag augcugcggc gccauugggg gcugaacgga     2220

guugccgcgc uuacagacuc cgauaagcag accgucaaag ccaagaauag aacggaccac     2280

cggcaucacg cuauugaugc cgcuguggug gccgccaccg acagaggccu caucaaacgc     2340

aucgcugaua uggcgaagcg ggacgaauuu aacggggccg aggaagucgc uagaucuauu     2400

cccccgccuu gggagggcuu ccgcgaugac gugagcgccc agguuacacg gaucauugug     2460

ucccacagag ccgaucaugg acgcaucgac cccgcugcca gaaagagcgg ccgggacucu     2520

accuccgggc agcugcacaa ugauacagcg uacggaauug gccgcgacgg gaccgucgug     2580

agcagaaaac cccuggcuuc ucucuccccu aacgauaucg uugugacgaa gcggggagcc     2640

aauauucgcg acgucgaucu gcaaaaacau cuugccagag ugacacacgg ccuggacggg     2700

aaggcuuuug aggccgcgcu ccuggcuuuc gccgccagcc cgauccugcc cgauaacacc     2760

gacaauccuu acuuuggccu ucggcgcguu agacucgaag agacacugca ggaauccgcu     2820

cggguggagg ucgccgauca ggaaggaaga cccuauaagg cuuacaaggg cgacucuaac     2880

cauugcuaug agaucuggug ucugccugau gggaaaauua agccccaggu ggugaccacc     2940

uacgaggccc accaaagcgg agcggaaaag aaaccucacc ccgccgcuaa gcgccucaug     3000

cggaucuuca aaagagacau ggucaugcug gagcgcgaug gcgaaacaau uaucggguau     3060

guucagaaga ugaaacagaa cggauccauu uuuguggccc cgcauacgga ggcuaaugcc     3120

gacgcccggg acagagaucc uaaggacgau uucaaacuua uccagcuggg cgcuggcucu     3180

cucgugaaag cgaaggcccg gcgcgucauc guggacgaga uggggagacu gcgggauccc     3240

ggaccucccc uguga                                                      3255


<210>  3884
<211>  3258
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3884
auggacaagg gcaccaacag caucgggugg gccgcucuga gacucgauga gcagggagaa       60

cccgugggca uuaucgaggc cgggguccgg auuuacgcgg acggacgcga accuaaaucc      120

ggcacaucuc uggcugaggg gagacggauc gcccgcggaa ugagcagacg gcgcgauaga      180

uauaagcggc gcagacggac gguucuuaau acccugacag aguucggccu caugccggcc      240

accaaagcug cccgcaaggc ucugguggcc gagacaaacg acagacgguu uggcgaggug      300

gcguccaaug ucuacgcccu ucgcgcuaga gcccuggaug aaaaacuccc ccuguaucaa      360

cuggggcggg cucucuucca ccugaaccag agacgcggau uuaagucuaa ucggaagacc      420

gacaaaagag auaacgacca gggcaagauu gccagcgcga ucucccgccu ugggcggaaa      480

cuggaugagg ccggagcuag aacacucggc gaguuccugc auaagcagca cgccgcuggg      540

aaaugggugc gcguucggcc uucuagagug cccggagaag acgauaagga ggaagacggc      600

uacggcuuuu auccugagcg cagcuuccuu gaacgggagu uugccgccau cugggaagcu      660

caagcggccu ucuaccccga gcugcucacc gaggaaagac gcgaucaucu gcacuggguc      720

auguuuuucc agcggccucu gaagagaccg acggugggga aaugcuccuu ucaucccgac      780

gaggaccggc ucccuaaggc ucacccccug uuccaggaau uucgccuuau uaaagagcug      840

aaugaacucg agguugugca cagaggagau cggagagaga agcugacccc ugaccagcgc      900

gaucggcuuu ucaaccaacu gagaacacag cgcagcucug ccuucaccgc ccucagaaaa      960

acaaucaagg cucccaccgg cacgcgguuu aauaaagaau ccgacgcccg cgauaagcug     1020

aagggggacg aggucgcggc ugaaaugagc cauaaagaua gauucggaca ggccuggauu     1080

ggcaugccgc uggccgagcg gugggcugug guggccaugc uccuggacac agaagauccu     1140

cuugagguca aggcuuggcu ggaggaacgc uauggguuug ccgcggacag aaucgaugcc     1200

auugcugccg cuaaacuccc caccggauac ggccggcugg gcgagacagc ccuucgcgac     1260

augcuggacg aacucagaaa cgggaccguu aaggaugacg cgggacggga uguggugcug     1320

accgagcgcc aggccaugga caauaucugg ggcgauaacc cugcucugca aagagccguc     1380

acagacgcuc gggagcuccc ucccuaccag gaagugcugg ggagacgcau ucccccggga     1440

acgggcaaac ccgccgaucc uuaugacguu cagaaggggc ggcuuaccaa ucccacagug     1500

cacaucggcc ugaaccaacu caagagaauc gucaaugugc ugaugcgccg gcauggaaga     1560

ccugagcaga uugcccugga acuugcucgc gaucucaaac ugaacgagga acagaaggcg     1620

gagaaaaacc gggagaucgc cagaaauacc aaggcugccg aagcccgcgg caaaaagcug     1680

gaggaacucg ggcacgcuaa caauggauac aaccggguuc ugcuuaagcu gugggaggac     1740

cucaaucuga aacagcccga aaacagagag uguauuuauu gcggcaaggc caucgggauu     1800

ggaacacugc uuucuaauca aguggauauc gaccacaucc ucccgugguc caaaacccug     1860

gaugacagcc aggcgaaccg gauugucugu cauguggagu gcaaucgcca gaagggcaac     1920

cacgcuccug ccgaaguccc ccaguggcau gacagauacg augacauccu ggcccgggcu     1980

gagcgccucc cuagaaauaa agccuggcgg uucgggagag aagcgaugaa gaaguuuaaa     2040

gaugacgagu cuuuccuggc ucgccaacuu acggauacac aguaucuguc ccggcucgcc     2100

cacgaauacc ugggcgcccu guaucccgac gaggagccug augaaugggg aggccccgcu     2160

agacgcaacc augugcgggu uauuccgggg agaaugaccg agaugcuucg ccggcagugg     2220

ggacucaaug ccaucauuag cgaccacaac cagaagaaua gaaacgauca ccgccaucac     2280

gcuaucgacg ccaucguggu cggcgugacc acaagacggc ugcugcaacg cauugcgacc     2340

guugccgggg gaagagaagc ucucgcccug gaggagacaa ucggcaaaau ugaaccuccc     2400

uggcccgggu uuuacgauga cgugaaggcu gccaucgauc ggauugucgu gucccauaaa     2460

ccugaccacg gcucucuucc gaagcccgga aaaccuggcc gcacggccgg gcagcugcac     2520

aacgacaccg cuuacggauu cacaggcgag gcgagaaaug gggugcccau caugguucau     2580

cgggucccuu uugccagccu caccgaaaag gauauucccu ccaucagaga ccgccagcug     2640

gcugaugccc uguaugccgc uaucagcgga auuucuggca aggccgagcu ugcggcugcc     2700

cucgcccggu ucuccgaaag cgggccuuac aaaggcauca gacacgugcg cguggucgag     2760

cccgugucug uuauugcuau caaggaccag gccggaaaag cguauaaggg cuacaaaggg     2820

gaugcuaacu aucgguacga cgugugggag cugccgaaug gaaaguggac acaugaaguc     2880

gugagcaugu uuaacgccca cagaccugau uggcaauccg agauucgguc uaagaauccc     2940

gccgcucgca aaguucugag ccuccaucag aacgacaugg uggccuauga acacccucag     3000

gauggcuaca ccaucgggag agucgugaag uucuccgcug gaggccaaau ggcccuggcg     3060

ggcaaucggg aagggggagc ccuuaaaagc cgcgacgcug aucccgaaga cagauuucgg     3120

uacuucuaua agucuggcgg ggccaugcgc gacguucagc ugagacaggu gcgggucgau     3180

gagacgggac gcguguucga cccgggcccu caggauagag agucccggaa cgcuagaaaa     3240

caaaaugacg ccggguga                                                   3258


<210>  3885
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3885
augagauaca agaucggccu ggacauuggg aucaccagcg ugggaugggc cgucaugaac       60

cucgauauuc cccggaucga ggaccugggc guucgcauuu ucgauagagc ugaaaauccu      120

cagacagggg agucccuugc ccugccgcgg cgccucgcga gaucugcucg gcgcagacug      180

cggcgcagaa aacaccggcu ugaacgcauc agacggcugg ugauccgcga gggcauucuc      240

acgaaggagg aacuggacaa acuguuugag gaaaagcaug agaucgaugu guggcaacuc      300

agagucgaag cccuggaccg gaaacuuaac aaugaugagc uggccagagu gcuccugcac      360

cuugcuaagc gccggggauu caagagcaac agaaaauccg agcgcucuaa uaaggaaaac      420

agcaccaugc ugaaacauau ugaggaaaau cgggccaucc ucuccucuua uagaacaguu      480

ggggagauga uugugaagga ccccaaauuu gcucugcaca agcgcaacaa gggagaaaau      540

uacaccaaca caaucgcccg ggaugaccug gagagagaga uucgccucau cuucagcaaa      600

cagcgggaau uuggcaauau guccugcacc gaggaguucg aaaacgaaua uaucacaauu      660

ugggcgagcc agagaccugu cgccucuaag gaugacaucg agaaaaaggu gggcuuuugu      720

accuucgagc ccaaagaaaa gcgcgcuccu aaagccacgu acaccuuuca guccuucauu      780

gcuugggagc auaucaauaa gcugcggcuu auuagccccu cuggggccag aggacugaca      840

gaugaggaac ggcgccuccu guaugaacaa gcguuucaga agaacaaaau caccuaccac      900

gacaucagaa cacuucugca ccucccugac gauaccuauu ucaagggcau uguuuacgac      960

agaggggagu ccagaaaaca gaaugagaac auccgcuuuc uggaacugga ugccuaccau     1020

cagauucgga aggcugugga caaagucuau ggaaagggca agucuagcuc cuuccucccg     1080

aucgauuucg acacguuugg guacgcccug acacuuuuca aagaugacgc ugauauucac     1140

agcuaucuga gaaacgagua cgaacaaaau ggaaagcgca ugcccaaccu cgccaauaaa     1200

guguaugaca acgagcugau cgaggaacuu cugaaucucu ccuuuaccaa guucggccau     1260

cugucucuga aagcccucag aagcauccug ccuuacaugg agcagggcga aguguauucc     1320

agcgcuugcg agcgggcggg guacacauuu accggaccca agaagaaaca gaagaccaug     1380

cuucugccua acauuccccc gaucgccaau ccugucguua ugcgcgcucu cacacaggcc     1440

agaaaagugg ugaacgccau uaucaagaaa uacggcucuc ccgucuccau ucacaucgaa     1500

cuggcucggg aucugagcca aacguucgac gagcgcagaa agaccaagaa agagcaggac     1560

gaaaaucgga agaaaaacga gacagccauu cgccagcuua uggaauaugg gcucacccug     1620

aauccuacag gacacgauau cgugaaguuu aaacuguggu cugagcaaaa cggcagaugu     1680

gcguacagcc uccagcccau cgaaauugag cggcugcuug agcccgggua uguugaagug     1740

gaccauguca ucccuuacuc ccgcucucug gaugacagcu auaccaauaa ggugcucguu     1800

cugacgagag agaaccggga aaaaggaaau cggauuccgg cugaguaccu gggcgugggc     1860

acagaacgcu ggcagcaguu cgagaccuuu guccuuacca acaagcaauu cuccaagaaa     1920

aagcgggaua gacuccugcg ccugcacuau gacgagaacg aagagacaga auuuaaaaau     1980

cggaaccuca augauaccag auacaucagc cgcuucuuug ccaacuucau ucgggagcau     2040

cugaaguucg ccgagucuga cgauaaacag aagguguaca cagucaaugg gagagugacg     2100

gcucaccuuc gcucccggug ggaauuuaac aagaauagag aggaaagcga ccugcaucac     2160

gccguugaug cugugaucgu cgccugcacc acacccucug acaucgcgaa agugaccgcc     2220

uucuaucagc ggcgcgagca gaacaaggaa cucgcuaaaa agaaagagcc ucacuuuccc     2280

caaccuuggc cccauuucgc cgaugagcug agagcucggc uguccaagca cccgaaggaa     2340

agcauuaaag cccuuaaucu cggaaacuac gacgaccaga agcuggaguc ccugcagccu     2400

guuuuugugu cucgcaugcc caaaagaagc gucacaggcg cggcccauca ggaaacccuc     2460

cggagauaug uggggaucga ugagcgcucc ggaaagauuc aaacgguggu uaaaaccaag     2520

cugucugaaa ucaaacuuga cgcuagcggc cacuucccua uguacgggaa agaguccgau     2580

ccccggacau augaggccau uagacagcgc cugcucgaac acaauaacga cccuaagaag     2640

gcuuuucagg agccccugua caagccgaaa aagaauggcg aaccuggacc cgucauccgg     2700

accgugaaaa ucauugauac aaagaaccag gugauccccc ugaaugacgg caagaccguc     2760

gccuauaaca gcaacauugu gagaguugau guguucgaga aagacgggaa guacuacugu     2820

gucccugugu auacgaugga uaucaugaaa ggcauucuuc cgaauaaggc caucgaaccc     2880

aacaaaccuu acucugagug gaaggagaug acagaagacu auaccuuucg cuucucccuc     2940

uaccccaaug aucugauccg gauugagcug ccuagagaaa agacaguuaa aaccgcugcg     3000

ggagaggaga ucaacgugaa ggacgucuuu guguauuaca aaaccauuga uagcgccaau     3060

gggggacucg aacugaucuc ucaugaccac cgcuucuccc uuagaggcgu ugggagccgg     3120

acacugaagc gcuuugagaa auaucaagug gacguccucg gcaacauuua caaggugaga     3180

ggagaaaagc gggugggccu ggcuucuagc gcccauucca aacccgggaa gacgauccgc     3240

ccucugcaga gcaccagaga uuga                                            3264


<210>  3886
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3886
augcugagaa ugaaguacgc caucggccuc gacauuggga ucgcuagcgu gggauaugcc       60

guccuggcgc uugaucacga ggaaaacccc uggggcauua uccggcuggg gucccgcauu      120

uucgacguug cugagaaucc uaaagaugga gccucucucg cccugccgag acgggaagcu      180

cgcagcguga gacggcgccu uagacggcau caccaucgcc uggagagaau caagaaccuc      240

cugaucaaua ccgaauuuau uacaaaagac gagcugcucc accuguacga uggcaaccuu      300

uccgacgugu augagcugcg ggucaaggcc cucgauuacu cugugacgaa ugcugaacug      360

acccgcaucc uucugcaucu cgcccagaga cggggguuca aaagcaaccg caaguccgac      420

gcgaaugaua aggaggccgg acaacugcug gaagcuguuu cugccaacgc uaaaagaaug      480

caggagaauc acuaucggac agugggcgag auguuuuaca aggacgaucu cuucagcaaa      540

uauaagagaa acaaaggcgg gaccuaccug acaaccgucc accgcgacau gauugccgcg      600

gaagcccgga caauccuuga gaagcaguuu gcucugggaa auaacauuug cacccaggau      660

uucaucgaca aguaccucuc cauucugcuu agccaaagac aguuugacga aggccccggg      720

gagccuucuc ccuaugccgg aaaucagauc gcuaacauga ucggcaaaug uacguucgaa      780

ccuaaugagu accgcgccgc caaggcuucc uauagcuuug agcgguucaa ccugcuccag      840

aaagugaacc aucugagacu gcuccuggaa gggaagucua uugcgcuuga uaaugagcaa      900

cgcaaaaaga ucauugcccu ggcucacgac aaagccgauc uccgguacuc ccauaucaga      960

aaggcccugg gacuugacga aaagguucug uuuaacacca uuacauauaa ugaugacgug     1020

agcgucaucg agaaaaagac caaauucaac uuucuccagg cuuaccacca gaucaagaaa     1080

gugcuggccg aagauaugca gaagcugaca accgagcaac ucgacaauau uggccagauc     1140

cugucuacgu auaaguccga uaacaaacgc acagaggaac uuagcgcgcu gggccucgag     1200

aagaaaauua ucgacgcucu gcuggggauu aauggacuuu ccaaguucgc ccaccucucu     1260

cugaaagccc ugcggaagau caaccccuac cucgaagagg gcaagaucua caaugaggcu     1320

ugcaccgccg cuggguauga uuucaaagcc caugcgaaca cacagaagac cgagcugcuu     1380

ccugccuaca aagaggaaau ggacgacauu accagcccgg uggcuagacg cgccaucgcu     1440

caauccauua aggucaucaa ugccauuauc cgggagcaga aaggaagccc ccuguauauu     1500

aacaucgaac ucgcgagaga gauggccaag ggcuuugaug agcggacaca gaucgacaag     1560

gcuaauaaag aaaaccaggc caagaaugag cgcauuaugg aaagaauccg gacggaguuc     1620

cacaaagcua acccuaccgg gauggaucug auuaagcuga aacuuuggga agaacaagac     1680

ggaagaucuc ccuacuccca gaaggccauc agcauuaacc gccucuuuga gccuggcuau     1740

guugauaucg accauaucgu gcccuacucu auuagcuucg augacuccuu uaaaaauaag     1800

gugcuggucu ucucugauga aaaccgggac aagggcaaua gacugccgau cgccuaucuc     1860

cagagcaaau uuggggcuaa ggcggccgag aacuucauga uuugggugca guccaauauc     1920

aaagauuaca agaaacgcca aaagcugcuu aagagagaaa uuacagagga agacaucaac     1980

aaauuuaagg agcggaaucu gcaggauacc aaaacaauca gccgcuuccu cuacaacuau     2040

auuaaugacu accugcuguu ugcuccuucu gacaccggaa agaaaaagag aguuacggcc     2100

gugaacggca caaucaccgc cuaucuucgg aagcgcuggg ggauuaauaa aaucagagcu     2160

aacggagaua agcaccaugc cgucgacgcg gugguuauug cuuguaccac agauaaaaug     2220

aucaaggacc ucuccagcuu cucucgguac cacgagcugg aauauaccca cacagauacg     2280

gaguccuuuc uggugaauag ccucaccggc gaaauccuga aacgcuuccc cuacccuugg     2340

gaggacuuca gacccgaacu uauggcccgg cuguccgaua accccgccga cgcucuccgc     2400

aagcugaauc ugaucuuuua ucaugggauu gaucuuucua caauuaagcc uaucuucguc     2460

agcagaaugc cgcggcacaa agugaccggc gccgcgcaua aggcuacaau uaaauccgcc     2520

agaugccucg acaacggaau cgucauuugu aagaccucuc ugcagaaccu gaaacucgau     2580

aaggagggcg agaucgccaa uuacuacgcu cccgaaagcg acacgauccu guauaacgcc     2640

cuuaaagagc gccugcgguc cuacgauaau aagccugcua aggccuuugc ggaacccuuc     2700

uauaaaccua aggccgacgg gacccccgga ccgcucguga aaaagguuaa aguguacgag     2760

aagucuacac ugaacgucgc ugugcagcaa aauaccgccg uugcugacaa cgauucuaug     2820

gugagagucg acguguuuca cgugaagggc aauggguauu accugguucc uauuuauauc     2880

gccgauacac uuaaaaagga gcugcccaac aaagccauug ucgcuuacaa gccuuacgcg     2940

gacuggcccg ugauggauga cuccaauuuc aucuuuagcc ucuauccuaa cgaucugauu     3000

aaauucgaac acaagaauag cgugaaguuu gccaaaguca acaaggaguc cacccucaau     3060

gacuucuaca gcacgaaauc ugaaaucgug uauuuuaagg gaugcaacau caauacaggc     3120

gcuauuacca ucauuaacca ugagaacaca uacguuguga gcucccuggg ggucaaaacc     3180

cuucccagcc uggaaaagua ucaggccgau gugcucggca auuacaccaa gguuaaaaag     3240

gaggugcgca aaccguuccg guga                                            3264


<210>  3887
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3887
augagauaca agaucggccu ggacauuggg aucaccagcg ugggaugggc cgucauuaac       60

cucgauaucc cccggauuga ggaccugggc guucgcaucu ucgauagagc ugaaaauccu      120

cagacagggg agucccuugc ccugccgcgg cgccucgcga gaucugcucg gcgcagacug      180

cggcgcagaa aacaccggcu ugaacgcauc agacggcuga uuauccgcga gggcauucuc      240

acgaaggaag aacuggacaa acuguuugag gaaaagcaug agaucgaugu guggcaacuc      300

agaguggaag cccuggaccg gaaacuuaac aaugaugagc uggccagagu ccuccugcac      360

cuugcuaagc gccggggauu caagagcaac agaaaauccg agcgcucuaa uaaggaaaac      420

agcaccaugc ugaaacauau ugaggaaaau cgggccaucc ucuccucuua uagaacagug      480

ggggagauga uuguuaagga ccccaaauuu gcucugcaca agcgcaacaa gggagaaaau      540

uacaccaaca caaucgcccg ggaugaccug gagagagaga uccgccucau uuucagcaaa      600

cagcgggaau uuggcaauau guccugcacc gaggaguucg agaacgaaua uaucgcgauu      660

ugggccagcc agagaccugu ggcuucuaag gaugacaucg agaaaaaggu cggcuuuugu      720

acauucgagc ccaaagaaaa gcgcgccccu aaagcuaccu acacguuuca guccuucauu      780

gccugggagc auaucaauaa gcugcggcuu aucagccccu cugggaccag aggacugaca      840

gaugaggaac ggcgccuccu guaugaacaa gcguuucaga agaacaaaau uaccuaccac      900

gacaucagaa cacuucugca ccucccugac gauaccuauu ucaagggcau uguguacgac      960

agaggggagu ccagaaaaca gaaugagaac auccgcuuuc uggaacugga ugccuaccau     1020

cagauucgga aggcuguuga caaaguguau ggaaagggca agucuagcuc cuuccucccg     1080

aucgauuucg acacguuugg guacgcccug acacuuuuca aagaugacgc ugauaucaga     1140

agcuaucugc gcaacgagua cgaacaaaau ggaaagagaa ugcccaaccu cgccaauaaa     1200

gucuaugaca acgagcugau ugaggaacuu cugaaucucu ccuuuaccaa guucggccac     1260

cugucucuga aagcccuccg gagcauccug ccuuacaugg agcagggcga aguguauucc     1320

agcgcuugcg agcgcgcggg guacacauuu accggaccca agaagaaaca gaagaccaug     1380

cuucugccua acauuccccc gaucgccaau ccugugguca ugagagcucu cacacaggcc     1440

cggaaaguug ugaacgccau uaucaagaaa uacggcucuc ccguguccau ucauaucgaa     1500

cuggcucgcg aucugagcca aacguucgac gagagacgga agaccaagaa agagcaggac     1560

gaaaaucgca agaaaaacga gacagccauc agacagcuua uggaauaugg gcucacccug     1620

aauccuacag gacacgauau ugucaaguuu aaacuguggu cugagcaaaa cggccggugu     1680

gcguacagcc uccagcccau cgaaauugag cgccugcuug agcccgggua uaccgaagug     1740

gaccacguua ucccuuacuc cagaucucug gaugacagcu auacgaauaa ggugcucguc     1800

cugacaaaag agaaccggga aaagggaaau cggauuccgg cugaguaccu gggcgugggc     1860

accgaacgcu ggcagcaguu cgagaccuuu guucuuacaa acaagcaauu cuccaaaaag     1920

aaacgggaua gacuccugcg ccugcauuau gacgagaacg aagagaccga auuuaagaau     1980

cggaaccuca augauacaag auacaucagc cgcuucuuug ccaacuucau ccgggagcac     2040

cugaaauucg ccgagucuga cgauaagcag aagguguaca cggucaaugg gagagugacc     2100

gcucaucuuc gcucccggug ggaauuuaac aaaaauagag aggaaagcga ccugcaccau     2160

gccgucgaug cugugauugu ugccugcaca acccccucug acaucgcgaa ggugacagcc     2220

uucuaucagc ggcgcgagca gaacaaagaa cucgcuaaga aaaccgagcc ucacuuuccc     2280

caaccuuggc cccacuucgc cgaugagcug agagcucggc uguccaagca uccgaaggaa     2340

agcauuaaag cccuuaaucu cggaaacuac gacgaccaga agcuggaguc ccugcagccu     2400

gucuuugugu cucgcaugcc caaaagaagc guuacgggcg cggcccacca ggaaacccuc     2460

cggagauaug uggggaucga ugagcgcucc ggaaagauuc aaacagucgu gaaaaccaag     2520

cugucugaaa ucaaacuuga cgcuagcggc cauuucccua uguacgggaa agaguccgau     2580

ccccggacau augaggccau cagacagcgc cugcucgaac acaauaacga cccuaagaag     2640

gcuuuucagg agccccugua caagccgaaa aagaauggcg aaccuggacc cgugauucgg     2700

accguuaaaa ucauugauac gaagaaccag gucauccccc ugaaugacgg caagacagug     2760

gccuauaaca gcaacauugu gagagucgau guguucgaga aagacgggaa guacuacugu     2820

guuccugugu auaccaugga uaucaugaaa ggaauccuuc cgaauaaggc cauugaaccc     2880

aacaaaccuu acucugagug gaaggagaug acagaagacu auaccuuucg cuucucccuc     2940

uaccccaaug aucugauccg gauugagcug ccuagagaaa agaucauuaa aaccgcuggc     3000

ggggaggaga ucaagauuaa agaccucuuu gcguauuaca agacaaucca cagcggaacg     3060

gccggccugg aacuugucuc ucaugauugc uccuucagcc ugucuggggu gggcagccgc     3120

acccucaaaa gauuugagaa guaucaaguu gacgugcugg gaaacaucua caagguccgg     3180

ggcgaaaaac gcguggggcu ggcuuccagc gcccacucua agacaggaga gaccauuaga     3240

ccccuucagu ccacacggga cuga                                            3264


<210>  3888
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3888
augagauaca agaucggccu ggacauuggg aucaccagcg ugggaugggc cgucauuaac       60

cucgaucugc cccggaucga ggaccuuggc guucgcauuu ucgauagagc ugaaaauccu      120

cagacagggg agucccuggc ccucccgcgg cgccuggcga gaucugcucg gcgcagacuu      180

cggcgcagac ggcaccgccu ggaaagaauc cggcgccucc ugacgagaga gaacgugcug      240

acccaagagg aacucgacaa acuguuugag aagaaacaug aaaucgaugu guggcagcuu      300

cgggucgagg cccuggacag aaagcucaau aacgaugaac uggcccgcgu gcuucugcac      360

cucgcuaaac ggagaggauu caagagcaau cgcaaguccg agcggucuaa caaagagaau      420

agcacaaugc ugaagcauau ugaagagaac agauccaucc ugaccggcua ucgcacaguu      480

ggggaaauga uugugcggga ccccaaauuu guccuccaca agagaaauaa aggagagaac      540

uacaccaaua caaucgcccg cgaugaccug gaacgggaga uucagcuuau cuucgcuaga      600

cagcgcgagu uuggcaacau gauuugcacc gaagccuucg agaaugaaua uaucgcgauc      660

uggucuagcc aacggccugu ggccuccaag gaugacauug agaagaaagu uggcuuuugu      720

acguucgaac ccaaggagaa aagagcuccu aaggccaccu acacauuuca gagcuucauc      780

gcuugggagc auauuaacaa acugcggcuc gugucucccu ccgggacccg cgaacugaca      840

gaugaggaaa gacggcuucu guaugagcag gccuuucaga agaauaaacu caccuaccac      900

gacaucagaa cgcugcugca ccucccugac gauacauauu ucaagggaau ugucuacgac      960

uggggcgaac cgcugaagca aaacgagaac auccgcuuuc uugagcugga ugcguaccau     1020

cagauccgga aaaccgugga caagguguau gggagaggaa aaucuagcuc cuuccucccc     1080

auugauuucg acacauuugg cuacgcccug acccuuuuca aggaugacgc ucacauccgc     1140

agcuaucuga gaaaugaaua cgagcagaac gggaaacgga uugccaaucu cgcuaacaag     1200

gucuaugaua augaacugau cgaggagcug cucaaccugu cuuuuaccaa guucggacau     1260

cuuucccuga aagcccuccg caauauucug ccuuacaugg aacagggcga gguuuauagc     1320

uccgccugcg aaagagcugg cuacacauuu acggggccca ccaagaaaca aaagacagug     1380

cugcuuccua ccauuccccc gaucgcgaac ccugugguca ugcgggcccu cacacaggcu     1440

cgcaaagugg uuaaugccau caucaagaag uacggaucuc ccgugagcau ucacaucgag     1500

cuggccagag accuguccca gaccuucgau gaacggaaaa agacgaaaaa ggagcaagac     1560

gagaaccgca aaagaaauga aacagcuauu cggcagcuca ucgaguaugg ccugacccgc     1620

aacccuaccg ggcacgacau ugucaaguuu aagcugugga gcgaacagaa uggaagaugu     1680

gccuacucuc ugcagcccau cgagaucaaa cggcuccugg aacccggcua uguggagguu     1740

gaucauguga uuccuuacuc cagaagccug gacgauucuu auacaaacaa gguccuugug     1800

cucaccaaag agaaucgcga aaaggggaac cggaucccgg cggaguaccu gggagucggc     1860

acagaaagau ggcaacaguu cgagacguuu gugcugacca acaaacaguu cagcaagaaa     1920

aagcgcgacc ggcuccugag acuucacuau gaugaaaaug aggaggcuga auggaagaac     1980

cgcucccuga augacacacg guacauuucu agauuuuucg ccaacuuuau ccgcgagcau     2040

cucaaauucg ccgauagcga cgauaagcag aaaguuuaca ccgugaaugg cggggucaca     2100

gcucaccuuc gguccagaug ggacuucaac aagaaucggg aagagagcga ucugcaucac     2160

gccguggacg cugccauugu ugcgugcacc acgcuuucug auaucgcccg cgugaccgcu     2220

uuuuaucaaa gacgggagca ggccaaagaa cucgcuaaga agacagagcc ccacuucccu     2280

cagcccuggc cucauuuugc cgacgaacug cgcgcgagac uguccaaaaa ccccaaggag     2340

agcaucaaag cccucaaucu gggaaacuac gacaaugaaa aacuugaguc ucugcagccg     2400

gucuucgugu cccggaugcc uaagagaagc gugaccggcg cugcccacca agagacacuc     2460

cgccgguaua uugggaucga ugaaagaucc ggaaagauuc agaccguugu caagacgaaa     2520

cugucugaga ucaagcugga cgcuagcggc cauuuuccca uguacgggaa agaauccgau     2580

ccucgcacau augaggccau ucggcuuaga cuccuggaac acaacaauga cccccgcaag     2640

gccuuccagg agccucugua caaacccaag aagaacggcg agcucggacc gaucauccgg     2700

accgugaaaa uuaucgauac aaagaaucag gugauuccuc ugaacgacgg caaaaccguc     2760

gcuuauaacu cuaauaucgu gagaguugau guguuugaaa aggacgggaa auacuacugu     2820

guccccgugu auaccaugga uauuaugaag aaaguucuuc ccaacaaggc gaucgagccu     2880

aauaagccgu acagcgaaug gaaagagaug acagaagacu auacguuccg cuuuucccug     2940

uaccccaacg aucucaucag aauugagcug ccucgggaga agaccgugaa aacaaccgcc     3000

ggacacgaaa ucaauauuaa ggacgucuuc guguauuaca aaacaaucga uagcgcuacc     3060

ggcgggcugg agcuuauuuc ucaugacaac uccuuuagcc ugcgcggaau cggcucuaga     3120

acgcucaagc gguucgaaaa guaucaaguu gacgugcugg ggaauauuua caaaguccgc     3180

ggcgagaaga gagugggacu cgccuccagc gcccaccccg agacacggga aaccaucaga     3240

ccucugcagu cuacccgcga uuga                                            3264


<210>  3889
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3889
augaagaaac uggugcucgg ccuggacauc ggggucgcca gcguuggaug gggcauuauc       60

gaugcugaga acggggacgu gguggauacc ggagucagac uuuucuccuc ucgggacgcc      120

agcgaaaaug agucccgcag aacaucucgg cagagccgca gacugucccg gcgcaagaaa      180

cacagacggg aacgcauuaa gcaacuccug cuugcgcaug auauggacau cugcgauauu      240

gugaacgaga aucccuacga acugagaguu aaaggccuca acgagaagcu ggaggugucu      300

gaaaucuaug ucgcucugac gcaccucgcc aagcggcgcg gggugagcua ccuggacgau      360

guuguggagg aaggaggcac caucaaaucc gagcaucuua auaagaacau ggaggaaauu      420

aaaagcggca agaugccuug ugagauccag cuggaaagau uugagaaaua ugggcagguc      480

cggggaauga uugaaaucaa ucaggacaca gaggagucuu ccuuccucgu gaacauuuuu      540

cgcaccagcg auuacaucaa ggaagccaag acaauucugg cuacccaaca gaaauuccac      600

aagcagauca caaaugaguu uaucgacgaa uauauucaua ucauggccuc uaaaagagau      660

uacuucauug gcccggggaa cgagaagucc cggaccgacu auggagugua cagaacgaau      720

ggcgacaccu gggauaaccu uuuugaaauc cugauuggga aaugcagcgu cuuccccgag      780

gagacacgcg cugcccgguc uuccuacguu gcgcaggaau uuaaucuccu gaacgaccug      840

aauaaccuca agaucagcaa ccuggagucc ggaaaacuuu cugccagcca aaagcacgau      900

aucguggaaa aggugaaaaa uuccaagaaa uucggcacca aggaccugcu caaauauauu      960

gcuagcgucu gugagugcaa gaucgaagau auuggcgggu guagagugga caagaaccgc     1020

aaaccuauca ugcacacauu ugagaauuac cggaagguuc auaacgcccu gaaugaggcu     1080

ggaguggauc uuagacuguc ugaaaccgac uaugaugagc ucaugaaaau uaugacgcug     1140

aacuccgaaa cacuggagau caagaaacag ugcaaggaga agcuccccca gcugaccgaa     1200

gacuaccuua augucugucu ggugcucaac aaaaagggcg ccuucagcgg guggcacgcg     1260

cugucucuua aagccaugaa gcugaugaug ccugagcucu auggaacaag caaaaaucag     1320

augcaaauca uucauaccau gcagcugggc aaggcuuacc ugaagccccu caaccagcaa     1380

aaauaucuua auuccgaagc cauccuugag gaaauuuaca acccuaucgc ugcccgcucu     1440

auucggcaga ccaucaaggu uacaaaugcc auucugaaaa aguacgggca ccucgauagc     1500

gugaucaucg agaugccgag agacuaucag acguccgagg acgaacgcaa aaugauugag     1560

aagaugcaga aagaaaacga gaaggaaaag aaagcugcgc ugaagcgggc cagagaggag     1620

uaccccuuuc cugaugaggc uuuccgcaau cacaaagaac uggccaccaa gcugcggcuc     1680

ugguaucaac aggaccaguu cugcgucuac agcggaagaa aaaucucugu ggucgaucug     1740

gugcggaacc ccaaccaguu ugaaauugac cauaucauuc cucaauccgu uagccuggau     1800

gacucucuca auaacaaggu gcuguguuuc uccgaggaaa aucagaagaa aggccagcag     1860

acacccuuuu auuaccacaa gggcaaaagc gggaacugga ccuaugagca auacaaacuu     1920

uucgucgaga aacuguuuga uaagaagaaa aucuccaaua agaaacgcga acuccugcug     1980

uucgaggaag acaucaacaa gugggaugug agacggggau uuauugccag aaaucuuguu     2040

gacacacgcu acgcuucucg ggugauccuc aacagcuauc agcaguacuu cggcaaucau     2100

gagaugggga ccaaagucaa gguggugaga ggaaaguuua cgucccagcu gcgcaaaaag     2160

uggagaauuu ucaaagaucg gaacgagucu cacagccauc acgccaucga cgcgcugauu     2220

guugcugcca cagccaaucu ccgccugugg aagaaaaaca cccuuggcgu cuccgaugaa     2280

gggaaggugu augacaaaga gaccggcgaa auccugccgu uugagaagcu cacagaaagc     2340

gaguacaaug agaugaugaa caaggaaccu uaucugaauu ucaaaaccaa gcugcaaaac     2400

aucgagacaa aagugaagua cucuuauaga gucuccaaaa aggugaaccg gaagauuagc     2460

gacgcuacga ucuacucuac ccgcgaaaga gauggaauug acuacaucgu uggcaaaauu     2520

aagaauaucu augaugugaa aacauacaag gaccaguuuc uuaaacggua uaagaagaac     2580

aaagagcagu uccucaugua ccaacacgau ccccagaccu ucgccauccu ggaaaauauu     2640

auggcuacau aucaggacgc cgagaacccu uuucuggcgu acaagaaaga guaugggguc     2700

auccgcaagu acuccaaaaa gggaggcggg agccccguga ccucucucaa guucauugau     2760

aaaguucugg gacagaagau cgaccuuacg gccaaaaauc aagcugcccu gcccaagaac     2820

aaaaaggugg uccuccagag cauuaccccu uacagagcug auguguauua cgacgaaauc     2880

gauaagcggu aucaucugau uggcguuaaa uacucccugu uuagcuucuc uaagcagggg     2940

uaugugauca auccgaucgc cuaccagaaa cuucucgaga aggaacaaau ucacuccgag     3000

augaaauuuu gcuucagccu guaucgcgaa gacaucauug aguuuggcga ggaucccgac     3060

ucccugacaa gauaccgguu cuggucuaag aacgaaagcg gaaagaauaa gaucgagguc     3120

aagccuauug aaaaacccaa cuuugagaag cagaccgugc ucacaaucac caaaaagauc     3180

aaaauucuga auaaguacca gacggacgug cuuggcaacu ccuucaaggc gcaggaggaa     3240

aaagauccua gaaauaucga guga                                            3264


<210>  3890
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3890
augagauaca agaucggccu ggacauuggg aucaccagcg ugggaugggc cgucaugaac       60

cucgauauuc cccggaucga ggaccugggc guucgcauuu ucgauagagc ugaaaauccu      120

cagacagggg agucccuugc ccugccgcgg cgccucgcga gaucugcucg gcgcagacug      180

cggcgcagaa aacaccggcu ugaacgcauc agacggcugg ugauccgcga gggcauucuc      240

acgaaggaag aacuggacaa acuguuugag gaaaagcaug agaucgaugu guggcaacuc      300

agagucgaag cccuggaccg gaaacuuaac aaugaugagc uggccagagu gcuccugcac      360

cuugcuaagc gccggggauu caagagcaac agaaaauccg agcgcucuaa uaaggaaaac      420

agcaccaugc ugaaacauau ugaggaaaau cgggccaucc ucuccucuua uagaacaguu      480

ggggagauga uugugaagga ccccaaauuu gcucugcaca agcgcaacaa gggagaaaau      540

uacaccaaca caaucgcccg ggaugaccug gagagagaga uucgccucau cuucagcaaa      600

cagcgggaau uuggcaauau guccugcacc gaggaguucg agaacgaaua uaucacaauu      660

ugggcgagcc agagaccugu cgccucuaag gaugacaucg agaaaaaggu gggcuuuugu      720

accuucgagc ccaaagaaaa gcgcgcuccu aaagccacgu acaccuuuca guccuucauu      780

gcuugggagc auaucaauaa gcugcggcuu auuagccccu cuggggccag aggacugaca      840

gaugaggaac ggcgccuccu guaugaacaa gcguuucaga agaacaaaau caccuaccac      900

gacaucagaa cacuucugca ccucccugac gauaccuauu ucaagggcau uguuuacgac      960

agaggggagu ccagaaaaca gaaugagaac auccgcuuuc uggaacugga ugccuaccau     1020

cagauucgga aggcugugga caaagucuau ggaaagggca agucuagcuc cuuccucccg     1080

aucgauuucg acacguuugg guacgcccug acacuuuuca aagaugacgc ugauauucac     1140

agcuaucuga gaaacgagua cgaacaaaau ggaaagcgca ugcccaaccu cgccaauaaa     1200

guguaugaca acgagcugau cgaggaacuu cugaaucucu ccuuuaccaa guucggccau     1260

cugucucuga aagcccucag aagcauccug ccuuacaugg agcagggcga aguguauucc     1320

agcgcuugcg agcgggcggg guacacauuu accggaccca agaagaaaca gaagaccaug     1380

cuucugccua acauuccccc gaucgccaau ccugucguua ugcgcgcucu cacacaggcc     1440

agaaaagugg ugaacgccau uaucaagaaa uacggcucuc ccgucuccau ucacaucgaa     1500

cuggcucggg aucugagcca aacguucgac gagcgcagaa agaccaagaa agagcaggac     1560

gaaaaucgga agaaaaacga gacagccauu cgccagcuua uggaauaugg gcucacccug     1620

aauccuacag gacacgauau cgugaaguuu aaacuguggu cugagcaaaa cggcagaugu     1680

gcguacagcc uccagcccau cgaaauugag cggcugcuug agcccgggua uguugaagug     1740

gaccauguca ucccuuacuc ccgcucucug gaugacagcu auaccaauaa ggugcucguu     1800

cugacgagag agaaccggga aaaaggaaau cggauuccgg cugaguaccu gggcgugggc     1860

acagaacgcu ggcagcaguu cgagaccuuu guccuuacca acaagcaauu cuccaagaaa     1920

aagcgggaua gacuccugcg ccugcacuau gacgagaacg aagagacaga auuuaaaaau     1980

cggaaccuca augauaccag auacaucagc cgcuucuuug ccaacuucau ucgggagcau     2040

cugaaguucg ccgagucuga cgauaaacag aagguguaca cagucaaugg gagagugacg     2100

gcucaccuuc gcucccggug ggaauuuaac aagaauagag aggaaagcga ccugcaucac     2160

gccguugaug cugccaucgu ggcgugcacc acacccucug acaucgccaa agucaccgcu     2220

uucuaucagc ggcgcgagca gaacaaggaa cucgccaaaa agacagagcc ucacuuuccc     2280

caaccuuggc cccauuucgc ugaugagcug agagcccggc uguccaaaca cccgaaggaa     2340

agcauuaagg cgcuuaaucu cggaaacuac gacgaccaga aacuggaguc ccugcagccu     2400

guguuuguuu cucgcaugcc caagagaagc gugaccggcg ccgcucauca ggaaacgcuc     2460

cggagauaug ucgggaucga ugagcgcucc ggaaaaauuc aaaccguggu gaagacaaaa     2520

cugucugaaa ucaaacuuga cgccagcggc cacuucccua uguacgggaa ggaguccgau     2580

ccccggaccu augaggcuau uagacagcgc cugcucgaac acaauaacga cccuaagaag     2640

gccuuucagg agccccugua caaaccgaag aaaaauggcg aaccuggacc cguuauccgg     2700

acagucaaga ucauugauac caaaaaccag gugauccccc ugaaugacgg caagacggug     2760

gccuauaaca gcaacauugu cagaguggau guuuucgaga aggacgggaa auacuacugu     2820

gugccugucu auacaaugga uaucaugaag ggcauucuuc cgaauaaagc uaucgaaccc     2880

aacaagccuu acucugagug gaaagagaug accgaagacu auacauuucg cuucucccuc     2940

uaccccaaug aucugauccg gauugagcug ccuagagaaa agaccgugaa gaccgcggcc     3000

ggagaggaga ucaacguuaa agacguguuu gucuauuaca agacaauuga uagcgcuaau     3060

gggggacucg aacugaucuc ucaugaccac cgcuucuccc uuagaggcgu ggggagccgg     3120

acgcugaaac gcuuugagaa guaucaaguu gacgugcucg gcaacauuua caaagucaga     3180

ggagaaaagc gggugggccu ggccucuagc gcccauucca agaccgggga gacagugcgc     3240

ccccugcaga gcaccagaga uuga                                            3264


<210>  3891
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3891
augagauaca agaucggccu ggacauuggg aucaccagcg ugggaugggc cgucaugaac       60

cucgauauuc cccggaucga ggaccugggc guucgcauuu ucgauagagc ugaaaauccu      120

cagacagggg agucccuugc ccugccgcgg cgccucgcga gaucugcucg gcgcagacug      180

cggcgcagaa aacaccggcu ugaacgcauc agacggcugg ugauccgcga gggcauucuc      240

acgaaggaag aacuggacaa acuguuugag gaaaagcaug agaucgaugu guggcaacuc      300

agagucgaag cccuggaccg gaaacuuaac aaugaugagc uggccagagu gcuccugcac      360

cuugcuaagc gccggggauu caagagcaac agaaaauccg agcgcucuaa uaaggaaaac      420

agcaccaugc ugaaacauau ugaggaaaau cgggccaucc ucuccucuua uagaacaguu      480

ggggagauga uugugaagga ccccaaauuu gcucugcaca agcgcaacaa gggagaaaau      540

uacaccaaca caaucgcccg ggaugaccug gagagagaga uucgccucau cuucagcaaa      600

cagcgggaau uuggcaauau guccugcacc gaggaguucg agaacgaaua uaucacaauu      660

ugggcgagcc agagaccugu cgccucuaag gaugacaucg agaaaaaggu gggcuuuugu      720

accuucgagc ccaaagaaaa gcgcgcuccu aaagccacgu acaccuuuca guccuucauu      780

gcuugggagc auaucaauaa gcugcggcuu auuagccccu cuggggccag aggacugaca      840

gaugaggaac ggcgccuccu guaugaacaa gcguuucaga agaacaaaau caccuaccac      900

gacaucagaa cacuucugca ccucccugac gauaccuauu ucaagggcau uguuuacgac      960

agaggggagu ccagaaaaca gaaugagaac auccgcuuuc uggaacugga ugccuaccau     1020

cagauucgga aggcugugga caaagucuau ggaaagggca agucuagcuc cuuccucccg     1080

aucgauuucg acacguuugg guacgcccug acacuuuuca aagaugacgc ugauauucac     1140

agcuaucuga gaaacgagua cgaacaaaau ggaaagcgca ugcccaaccu cgccaauaaa     1200

guguaugaca acgagcugau cgaggaacuu cugaaucucu ccuuuaccaa guucggccau     1260

cugucucuga aagcccucag aagcauccug ccuuacaugg agcagggcga aguguauucc     1320

agcgcuugcg agcgggcggg guacacauuu accggaccca agaagaaaca gaagaccaug     1380

cuucugccua acauuccccc gaucgccaau ccugucguua ugcgcgcucu cacacaggcc     1440

agaaaagugg ugaacgccau uaucaagaaa uacggcucuc ccgucuccau ucacaucgaa     1500

cuggcucggg aucugagcca aacguucgac gagcgcagaa agaccaagaa agagcaggac     1560

gaaaaucgga agaaaaacga gacagccauu cgccagcuua uggaauaugg gcucacccug     1620

aauccuacag gacacgauau cgugaaguuu aaacuguggu cugagcaaaa cggcagaugu     1680

gcguacagcc uccagcccau cgaaauugag cggcugcuug agcccgggua uguugaagug     1740

gaccauguca ucccuuacuc ccgcucucug gaugacagcu auaccaauaa ggugcucguu     1800

cugacgagag agaaccggga aaaaggaaau cggauuccgg cugaguaccu gggcgugggc     1860

acagaacgcu ggcagcaguu cgagaccuuu guccuuacca acaagcaauu cuccaagaaa     1920

aagcgggaua gacuccugcg ccugcacuau gacgagaacg aagagacaga auuuaaaaau     1980

cggaaccuca augauaccag auacaucagc cgcuucuuug ccaacuucau ucgggagcau     2040

cugaaguucg ccgagucuga cgauaaacag aagguguaca cagucaaugg gagagugacg     2100

gcucaccuuc gcucccggug ggaauuuaac aagaauagag aggaaagcga ccugcaucac     2160

gccguugaug cugugaucgu cgccugcacc acacccucug acaucgcgaa agugaccgcc     2220

uucuaucagc ggcgcgagca gaacaaggaa cucgcuaaaa agacagagcc ucacuuuccc     2280

caaccuuggc cccauuucgc cgaugagcug agagcucggc uguccaaaca cccgaaggaa     2340

agcauuaagg cccuuaaucu cggaaacuac gacgaccaga aacuggaguc ccugcagccu     2400

guuuuugugu cucgcaugcc caagagaagc gucaccggcg cggcccauca ggaaacgcuc     2460

cggagauaug uggggaucga ugagcgcucc ggaaaaauuc aaaccguggu uaagacaaaa     2520

cugucugaaa ucaaacuuga cgcuagcggc cacuucccua uguacgggaa ggaguccgau     2580

ccccggaccu augaggccau uagacagcgc cugcucgaac acaauaacga cccuaagaag     2640

gcuuuucagg agccccugua caaaccgaag aaaaauggcg aaccuggacc cgucauccgg     2700

acagugaaga ucauugauac caaaaaccag gugauccccc ugaaugacgg caagacgguc     2760

gccuauaaca gcaacauugu gagaguugau guguucgaga aggacgggaa auacuacugu     2820

gucccugugu auacaaugga uaucaugaag ggcauucuuc cgaauaaagc caucgaaccc     2880

aacaagccuu acucugagug gaaagagaug accgaagacu auacauuucg cuucucccuc     2940

uaccccaaug aucugauccg gauugagcug ccuagagaaa agaccguuaa gaccgcugcg     3000

ggagaggaga ucaacgugaa agacgucuuu guguauuaca agacaauuga uagcgccaau     3060

gggggacucg aacugaucuc ucaugaccac cgcuucuccc uuagaggcgu ugggagccgg     3120

acgcugaaac gcuuugagaa guaucaagug gacguccucg gcaacauuua caaagugaga     3180

ggagaaaagc gggugggccu ggcuucuagc gcccauucca agcccgggaa aaccauccgc     3240

ccucugcaga gcacaagaga uuga                                            3264


<210>  3892
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3892
augagauaca agaucggccu ggacauuggg aucaccagcg ugggaugggc cgucauuaac       60

cucgauaucc cccggauuga ggaccugggc guucgcaucu ucgauagagc ugaaaauccu      120

cagacagggg agucccuugc ccugccgcgg cgccucgcga gaucugcucg gcgcagacug      180

cggcgcagaa aacaccggcu ugaacgcauc agacggcuga uuauccgcga gggcauucuc      240

acgaaggagg aacuggacaa acuguuugag gaaaagcaug agaucgaugu guggcaacuc      300

agaguggaag cccuggaccg gaaacuuaac aaugaugagc uggccagagu ccuccugcac      360

cuugcuaagc gccggggauu caagagcaac agaaaauccg agcgcucuaa uaaggaaaac      420

agcaccaugc ugaaacauau ugaggaaaau cgggccaucc ucuccgggua uagaacagug      480

ggagagauga uuguuaagga ccccaaauuu gcucugcaca agcgcaacaa gggcgaaaau      540

uacaccaaca caaucgcccg ggaugaccug gagcaugaga ucagacucau uuucucuaaa      600

cagcgcgaau uuggcaauau gagcugcacc gagaaguucg aaaacgagua uaucacaauu      660

ugggcguccc agcggccugu ggccagcaaa gaugacaucg aaaagaaagu cggguuuugu      720

accuucgagc ccaaggagaa aagagcuccu aaggccacgu acaccuuuca gucuuucauu      780

gcuugggaac acaucaauaa gcugcgccuu aucuuucccu ccggagcccg gggccugaca      840

gaugaggaaa gacggcuccu guaugagcaa gcguuccaga aaaacaagau uaccuaccac      900

gacauccgca cacuucugca ucucccugac gauaccuauu uuaaagggau uguguacgac      960

agaggagaaa gccggaagca gaaugagaac aucagauucc uggagcugga ugccuaccac     1020

cagauucgca aagcuguuga caagguguau ggcaagggga aaucuuccag cuuccucccg     1080

aucgauuuug acacguucgg auacgcccug acacuuuuua aggaugacgc ugauauccau     1140

ucuuaucugc ggaacgaaua cgagcaaaau ggcaaaagaa ugcccaaccu cgccaauaag     1200

gucuaugaca acgaacugau ugaggagcuu cugaaucucu ccuucaccaa auuuggccac     1260

cugagccuga aggcccuccg cuccauccug ccuuacaugg aacaggggga gguguauucu     1320

agcgcuugcg aaagagcggg auacacauuc accggcccca agaaaaagca gaaaaccaug     1380

cuucugccua acauuccccc gaucgccaau ccugugguca ugcgggcucu cacacaggcc     1440

cgcaagguug ugaacgccau uaucaaaaag uacggguccc ccgugagcau ucacaucgag     1500

cuggcuagag aucugucuca aacguuugac gaacggcgca agaccaaaaa ggagcaggac     1560

gagaaucgga aaaagaacga aacagccauc agacagcuua uggaguaugg acucacccug     1620

aauccuacag gccaugauau ugucaaauuc aagcuguggu ccgaacaaaa cgggcgcugu     1680

gcguacagcc uccagcccau cgagauugaa agacugcuug agcccggaua uguggagguu     1740

gaccacguga ucccuuacuc ucggagccug gaugacuccu auaccaauaa aguccucgug     1800

cugacgcgcg aaaacagaga gaagggcaau cggauuccgg cugaauaccu gggcguuggg     1860

acagagagau ggcagcaguu ugaaaccuuc gugcuuacca acaagcaauu uucuaaaaag     1920

aaacgcgauc ggcuccugag acugcauuau gacgagaacg aggaaacaga guucaagaau     1980

cgcaaccuca augauacccg guacaucagc agauuuuucg ccaacuucau ccgcgaacac     2040

cugaaauuug ccgaguccga cgauaagcag aaggucuaca cagugaaugg acgggucacg     2100

agccaucuua gaucucgcug ggacuucaac aaaaaucggg aggaauccga ucugcaccac     2160

gcuguggacg ccgcuauugu ugccugcacc acacccagcg auaucgcgaa ggugaccgcc     2220

uuuuaucaga gacgggagca gaacaaagaa cucgcuaaga aaacagagcc ucauuucccc     2280

caaccuuggc cccacuuugc cgacgaacug cgcgcuagac ugucuaagca uccgaaggag     2340

uccauuaaag cccuuaaucu cggcaacuac gacgaucaga agcuggagag ccugcagccu     2400

gucuucgugu cccggaugcc caaacgcucu guuaccgggg cggcccacca ggaaacgcuc     2460

agacgguaua ucggaauuga cgagagaagc ggcaagaucc aaaccguggu caaaacaaag     2520

cuguccgaaa ucaaacuuga ugcuucuggg cacuuuccua uguacggcaa agagagcgac     2580

ccccgcaccu augaagccau ucggcagaga cugcucgagc auaauaacga uccuaagaag     2640

gcuuuccagg agccccugua caagccgaaa aagaauggag aaccuggccc caucauucgc     2700

acagugaaaa ucauugacac caagaaccag gugauccccc ugaaugaugg gaagacgguu     2760

gccuauaacu ccaacaucgu ccggguggac guguuugaga aagauggaaa guacuacugu     2820

gucccugugu auacaaugga cauuaugaaa ggcauccuuc cgaauaaggc cauugaaccc     2880

aacaaaccuu acagcgagug gaaggaaaug accgaggauu auacauucag auuuucucuc     2940

uaccccaaug accugauccg cauugagcug ccucgggaaa agaccguuaa aaccacaacg     3000

ggggaggaaa ucaacgugaa ggaugucuuc guguauuaca aaaccauuga cuccgcuaca     3060

ggaggccucg agcugaucag ccacgacaau agauuuucuc uucgcggggu uggcuccaga     3120

acccugaagc gguucgagaa auaucaagug gauguccucg gaaacaucua caaggugcgc     3180

ggcgaaaaga gaguugggcu ggcgagcucu gcccauagca aaacaggaga gaccauucgg     3240

ccccugcagu ccacgcgcga cuga                                            3264


<210>  3893
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3893
augagauaca agaucggccu ggacauuggg aucaccagcg ugggaugggc cgucauuaac       60

cucgauaucc cccggauuga ggaccugggc guucgcaucu ucgauagagc ugaaaauccu      120

cagacagggg agucccuugc ccugccgcgg cgccucgcga gaucugcucg gcgcagacug      180

cggcgcagaa aacaccggcu ugaacgcauc agacggcugc ucauucgcga gggagugcug      240

acgaaggaag aacuggacaa acucuuugag gaaaagcaug agaucgaugu guggcaacug      300

agagucgaag cccuugaccg gaaacugaac aaugaugagc ucgugagagu ucugcuucac      360

cuggccaagc gccggggcuu caagagcaac agacaguccg agcgcucuaa uaaagaaaac      420

agcaccaugc ucaagcauau ugaggaaaau cgggcuaucc uguccgggua uagaacagug      480

ggagagauga uugucaaaga ccccaaguuu gcccugcaca aacgcaacaa gggcgaaaau      540

uacaccaaca caaucgcucg ggaugaccuc gagagagaga uucgccugau cuucucuaag      600

cagcgggaau uuggcaauau gagcugcacc gaggaguucg agaacgaaua uaucgccauu      660

ugggcguccc agagaccugu ggccagcaaa gaugacaucg agaagaaagu uggguuuugu      720

acauucgagc ccaaggaaaa acgcgcuccu aaggccaccu acacguuuca aucuuucauu      780

gcuugggagc auaucaauaa acuucggcug auuuccccca gcggaaccag aggccucaca      840

gaugaggaac ggcgccugcu uuaugaacag gccuuucaga agaacaagau caccuaccac      900

gacaucagaa cacugcucca ucugccugac gauaccuauu ucaaagggau uguguacgac      960

uggggagagu cucugaagca gaaugagaac auccgguuuc ucgaacugga ugcguaccac     1020

caaauuagaa aagccaucga caaggucuau ggcaaaggga aguccagcuc uuuccuuccg     1080

auugauuucg acacguuugg auacgcucug acacucuuca aggaugacgc cgauauccgc     1140

uccuaucugc ggaacgagua cgaacagaau ggcaaaagaa ucgcuaaccu ugccaauaag     1200

guguaugaca acgagcugau ugaggaacuc cugaaucuga gcuuuaccaa auucggccac     1260

cucucccuga aggcccuucg caacauccug cccuacaugg agcaggggga aguguauucu     1320

agcgcuugcg agaaagcggg auacacauuu accggcccua agaagaaaca gaagaccguc     1380

cuccugccca caauuccucc caucguuaau ccggugguga ugagagcccu gacgcaagcu     1440

cggaaagucg ugaacgccau uaucaagaaa uacggguccc cuguuagcau ucauaucgaa     1500

cuugcccgcg aucucucuca gaccuucgac gagagacgga agacaaagcg cgagcaggac     1560

gaaaauagaa aacggaacga gaccgcuauc cgccaacuga uggaauaugg acugacacuc     1620

aaucccaccg gccuggauau ugugaaguuu aaacuuuggu ccgagcagaa cgggagaugu     1680

gccuacagcc ugcagccuau cgaaauugag cggcuccugg agcccggaua ugucgaagug     1740

gaccacguua uccccuacuc ucgcagccug gaugacuccu auacgaauaa ggugcuuguc     1800

cucacaaaag agaacagaga aaagggcaau cggauuccug cggaguaccu gggcgugggg     1860

accgaaagau ggcagcaauu cgagaccuuu guccugacaa acaaacaguu cucuaagaag     1920

aaacgcgauc ggcuccugag acuucacuau gacgagaacg aagagaugga auuuaagagc     1980

cgcaaucuga acgauacccg guacaucucc agauucuuug cuaauuucau ccgcgagcau     2040

cucaaauucg ccgagagcga cgauaagcag aaaguguaca caguuaacgg acgggugacg     2100

ucucaccuga gaucccgcug ggacuuuaau aagaaccggg aagagagcga ucugcaucac     2160

gccgucgacg cugccauugu ggcuugcacc acaccgucug auaucgccaa gguuaccgcg     2220

uucuaucaga gacgggaaca aaauaaagag cuugccaaga aaacagaacc ccaguuuccu     2280

cagcccuggc cucauuucgc ugacgagcuc cgcgccagac uguccaagca ccccaaagag     2340

agcauuaagg cucugaaccu cggcaauuac gacgaucaga agcuggaauc ccuucaaccg     2400

guguuugucu cucggaugcc uaaacgcagc gugaccgggg ccgcgcacca ggagacgcug     2460

agacgguaua ucggaauuga cgaaagaucc ggcaagaucc agaccguggu uaaaacaaag     2520

cucucugaga ucaaacugga ugccagcggg cauuucccca uguacggcaa ggaauccgac     2580

ccucgcaccu augaggcuau ucggcagaga cugcuugagc acaacaauga ucccaagaaa     2640

gccuuucaag aaccucucua caagcccaaa aagaacggag agccgggccc uaucauucgc     2700

acagucaaaa ucauugacac caagaaucag gugauccccc ugaacgaugg gaaaacggug     2760

gcuuauaaua gcaacaucgu ccggauugac guguucgaaa aggauggaaa guacuacugu     2820

guucccgugu auacaaugga caucaugaaa ggcguccugc cuaacaaggc cauugagccg     2880

aauaaacccu acucugaaug gaaggagaug accgaggauu auacauuuag auucucccuc     2940

uacccuaacg accugauccg cauugaacuu ccccgggaga aaaccgugaa gaccgccgcu     3000

ggggaagaga ucaauauuaa ggauauguuu guuuauuaca aaacaaucga cagcgcgaac     3060

ggaggccugg agcucaucuc ucaugacaau uccuucagcc ugagaggggu gggcucucgc     3120

acgcugaaga gauuugaaaa auaucagguc gaugugcuug gaaacauuua caagguucgg     3180

ggcgagaaac gcguggggcu ggccagcucc gcucacagca agaccggaga aacaaucaga     3240

ccucuccaau cuacccggga cuga                                            3264


<210>  3894
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3894
augcccaagg aguucgaccc ucugaaaccg gccaaguaca gacucgcucu ggauaucggc       60

agcaaaucca uuggguuugc caucauuuuc cuuaaggaag acgugaacaa acugcucaag      120

cccuaugcga ucguccgguc uggaguucgc auuuuuggcg augggagaac cccugcugcc      180

cccggacagc ugggcagcuc ccuugccgcu gagcggcgcg ugaagagauc uauccggcgc      240

caauacgcca gaaaacggaa gcgccugcag agacuccugc ggaaucugaa agaacucggg      300

uucuuuccug acgauagcaa gcugugcaaa uccuucgagg accuuaaccc cuaugaacug      360

cgccacaagg gacucacaaa ggagcugaaa ccucaugagu uuggcagacu ucugaugcac      420

auggcuaaga aucggggcuu ccagucuaac cgcaaagugg aucuccuggc cgacaauauc      480

gauagccagg cgaaggccga aacguccgcu augaaaagcg ccauugcuac ccugcaaucu      540

agacucaacg gggaguuuga aacacuggga gccuaccuuu augagcgguu caagaaaggc      600

gagaccauca aguccagcaa gacacagaaa gggcagaagg cggaauacga guuuucuauu      660

uccagacgca uggucaaaca ggaguucgac cggcuguggg ccgcucaaga gcaguuuaau      720

ccggaacucu ucaacgagca ggccaagaau acccugaaag auacaaucuu cuuucagaga      780

aaccuucugc cccaaaaggu gggaaagugu accuuccucc cugagcauga acgcgcuagc      840

aaagcccugc ccucuuccca guauuuucgg auuaucagcg agcugaauaa gauuuccuuc      900

acggacgccg aaggccucau ccugccuauc ucucuugagc agagaaacaa acugcucaag      960

agccugcgca uuaaacaaaa acuuuccuuu gaucggauca gaaccgaacu gaaucucgac     1020

agcacaguuc ugauuaaccu ggagcacgcu ucuaaggacu ccaucgaggg ggauaagacc     1080

ggauaccucc ugagcaagaa agaauacuuc ggccccgcgu ggcgcgaccu uucucuggag     1140

acacaggaug ccauugugac cgcucuccug acgcaugaaa augaggccga acugguccag     1200

aaacuugugg acgauuuuaa caucgcccac gacguugcua aggcccucgc gaacgcucgg     1260

cuggagagcc aguauaugag acuguccaag gaggccaucg aacgccucau uccggagcug     1320

gaaaagggcg uggauucuca agacggguac aaagucccug ugaaguauca cgaggccaca     1380

gaggcugccg gauucggcca ucacagcgcc cuucaggaaa auugggcuga uuuucccacc     1440

cuggaccuca aacagcugca uuccgaugac aacgacaagu acacaguggc ggagcugccu     1500

cuucccuauu acgggaaagu caucacccgg cagguugccu ucggaaccgg cgaugugaau     1560

gaccccgugg aaaagagauu ugggaaaauu gcuaacccua caguccacau cugccucaau     1620

caagugcggg uucugcugga ugagauuauc gccaaguaug gauacccgua cgaagugaac     1680

aucgagcucg cucgcgagcu gaagcagaau cacgaacaga gacagcggag cauuaaacaa     1740

caggccgcga acaagaaaga gaaugaagag aucagacgcg aaauuaagca ggccuauaaa     1800

gucgagaagg cucggaucau uacggacucu gagaucucca gagccgauau ugaaaagugg     1860

cgccuuugga gacggagcaa aucuaagcug uguguguacu ccggcaaaaa gaucugcgag     1920

accaugaucc ucaacaauga aguugaggug gaccauauuc ugccccgcag cagaacaggc     1980

gauaacuccu aucagaauaa aguccuggug cuuaacaagg cuaaucggau caagucuaac     2040

cgcaccccuu gggaagccaa agaacucuuc gaggcccaag gguggagcua cgaagacauu     2100

cugaacagaa ugcgggcucu ggauaagcac cagcucuuuc gcuucgcgga gaauagcaug     2160

gacaucuggg auaaaaacua uagaucuuuc gaagcccggg cucugaauga cacacgcuac     2220

auuagcagag ucgugaagga uuaucuuaaa guuguguguc auaaggaccg gaucgucgug     2280

uccaacggag cccacaccgc ccugaucaga cgcgaguuug gccucaauga ccugcugggg     2340

aagaaaggag agaagaaccg ggaugaccau agacaccacg cuauugaugc cugcguuauc     2400

ggcgcgcuug accgcagcau gcuccggaaa cugcagauug cuauggaagu ggccgagaag     2460

caaaauucug ccagaaacuu cgucgggcug caggaacccu gguuuaauuu cuccacacag     2520

gugcgccgga gcaucggcca gauuuacgug ucucauaaac cuaaccacaa uuacgaggga     2580

gaaaucuuug aggcuuccau cuauggccuc agacugauua gcgauaagcg cacggagaaa     2640

uacguuggga ucaagcggaa ccccgaaacc ggaaagaccg acaaaccgaa gaaaucuguc     2700

guggugauua gcgauccuaa gacacaaaau agauauggcc uucggcauaa auuccacucc     2760

gaggggcugc ccaagaagua cuauggacuc uccucuucca gcgucuuuug ucuggaaauc     2820

uaccgcgacg agaaaggcaa cuggcaggcc uauaccauuc cuagauacga agcguaccgg     2880

aucgugaagc gcagaggggu uaaugcucug uaugauaaca aacgguccaa cuucgagggc     2940

agaccccuug ugaugcgccu gaugcgggac gauugcaucg ccuuugagaa gaauggaguc     3000

aaagaagugu accgcauuca gaagaucaac ggccucaaga aaaauauuac acugacgaga     3060

cacuucgacg ccaacguuga ugaccgguau agacgcaugc ucaaggcuca gaaagccgag     3120

aaggcugggg aagacuuuga uucugaggac cugugugaug accuuguguu cagcuccuuu     3180

ggagccucua gccuguacuc caauaaagcg cggaagguca ccaucucucc uaugggcgug     3240

cucagagauc ccggguucaa gggauga                                         3267


<210>  3895
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3895
augaagaaac uggugcucgg ccuggacauc ggggucgcca gcguuggaug gggcauuauc       60

gaugcugaga acggggacgu gcuugaugcc ggagugagac uguucuccuc ucgggacgcg      120

agcgaaaaug agucccgcag aaccucucgg cgccagagca gacggcucuc ccgcagaaag      180

aaacaccggc gcgaaagaau uaagcaacug cuucuggcuc augauaugga cgucugcgau      240

aucgugaacg agaaucccua ccagcuccgg guuaaaggcc ugaacgaaaa gcuggagauu      300

ucugagaucu auguggcccu cacacaccug gccaagcgca gaggggucag cuaccuugac      360

gaugugguug aagagggagg cgcuaucaaa uccgaacauc ugaauaagaa cauggaggag      420

auuaaaagcg gcaagaugcc uugugaaauc cagcucgagc gguuugaaaa auauggacag      480

gugcgcggga ugauugagau caaucaagac acggaagagu cuuccuuccu ggucaacauu      540

uuuagaacca gcgauuacau caaggaggcc aagacaauuc uugcuaccca gcagaaauuc      600

cacaagcaga ucacaaauga auuuaucgac gaguauauua acaucauggc cucuaaacgg      660

gauuacuuca uuggcccggg gaaugaaaag uccagaaccg acuauggagu guaccgcaca      720

aacggcgaca ccugggauaa ucuguuugag auccucauug ggaaaugcag cguguucccc      780

gaagagacgc gggcggccag aucuuccuac gucgcucaag aguuuaaccu gcugaaugac      840

cucaacaacc ugaagaucag cucccuugaa ucuggaaaac ugagcaccuc ccagaagcau      900

gauaucguug agaaggugaa aaauagcaag aaauucggca caaaggaccu ccugaaauau      960

auugccucug ugugugaaug caagaucgag aacauugaug gcugucgcaa ggacaaaaau     1020

cggaagccua ucaugcacac cuuugaaaac uacagaaaag uccacaaugc ucuuaacgag     1080

gccggggugg aucugcgccu cuccgaggcg gacuaugaug aacugaugaa gauuaugaca     1140

cugaauagcg agacccucga aaucaaaaag cagugcaagg agaaacugcc ccaucuuacg     1200

gaggacuacc ugaacguuug ucucgugcug aauaagaaag gagccuucuc uggcuggcac     1260

gcucuuagcc ugaaggccau gaaacucaug augccugaac uguaugggac auccaagaac     1320

cagaugcaaa ucauucauac caugcagcug ggaaaggcuu acaucaaacc ccucaaucag     1380

caaaaguauc ugaacucuga ggccauucuu gaagagaucu acaauccuau ugccgcucgg     1440

agcaucagac agacaauuaa agucaccaac gcgauccuga agaaauacgg cccgcucgau     1500

uccgugauca uugaaaugcc ccgcgacuau cagaccagcg aggacgagcg gaagaugauc     1560

gaaaagaugc agaaggagaa ugaaaaggag aaaaaggccg cucugaaaag agcccgcgaa     1620

gaguacccuu uucccgauga ggccuuccgg aaccacaagg aacuggcuac aaaacuuaga     1680

cucugguauc aacaggacca guucugcguu uacucugggc ggaagauuuc cguggucgau     1740

cuggugcgca acccuaauca guuugagauc gaccacauua ucccccaaag cgucucucug     1800

gaugacuccc ucaacaauaa ggugcugugu uucagcgaag agaaccagaa aaagggacag     1860

cagacgccgu uuuauuacca uaaaggcaag uccggcaauu ggaccuauga acaauacaaa     1920

cuuuucguug agaagcuguu ugauaagaaa aagaucucua acaaaaagag agagcuccug     1980

cuguucgaag aggacauuaa uaaaugggau gugcggagag gguuuaucgc ccgcaaccuu     2040

gucgacacac gguacgcgag cagagugauu cucaauuccu aucagcagua cuucggaaac     2100

cacgaaaugg gcaccaaggu uaaggugguc cgcgggaaau uuacaucuca gcugagaaag     2160

aaauggaaga ucuucaaaga ucggaaugag agccauuccc accaugcuau ugacgcccug     2220

aucguggccg cuaccgccaa ccuccgccug uggaagaaaa auaaacuugg aaucagcgau     2280

gagggcaagg uguaugacaa agaaacgggg gagauucugc cuuuugaaaa gcucacagcu     2340

ucugaguaca acgaaaugau gaauaaagag cccuaucuga acuucaagac caaacugcaa     2400

aacaucgaga ccaagguuaa guacuccuau aaagucagca agaaagugaa uagaaagauu     2460

ucuaacgaca caaucuacuc cacccgggaa cgcgauggca uugacuacau cgugggaaaa     2520

aucaagaaua uuuaugaugu caagacauac aaagaccagu uucuuaagag auauaaaaag     2580

aacaaagagc aguuccucau guaccaacac gauccucaga cguucgccau ccuggaaagc     2640

auuauggcga ccuaucccga cgccgagaau cccuuucugg cuuacaagaa ggaacacggc     2700

gugauccgga aauaugccaa gaacaaagcu ggggccccug uuacaucucu caaguucauu     2760

gauggagcgc ugggccagaa aaucgacgug accuacaaga auaaggccgc ccuuccgaaa     2820

aacaagaaag ucgugcugca gagcauuaag cccuaccgcg cugauguuua uuacgacgag     2880

gggaauaaaa gauaucaucu caucggagug aaguacuccc uguuuagcuu cucuaaacaa     2940

ggcuauguca ucaacccuau ugcuuaccag aagcugcuug agaaggaaca gauccacucc     3000

gagaugaaau uuugcuucag ccucuaucgg gaagauauua ucgaguccgg ggaagacccc     3060

gauucucuga cacgcuacag auuuuggagc aagaaugagu ccggcaaaaa caagauugag     3120

gugaaaccua ucgaaaagcc caauuucgag gaccggaagg uuaucaccau uacgaaaaag     3180

aucaaaauuc ugaacaagua ccagaccgac gugcucggaa auucuuuuaa agcccaagaa     3240

gagaaggauc cgagaagcau cgaguga                                         3267


<210>  3896
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3896
augaacuaca agaugggccu ggacaucggg auugccagcg ugggaugggc ugucaucaau       60

cucgaucuga aaagaauuga ggaccuuggc guucggaucu ucgauaaggc cgaacacccc      120

cagaacgggg agucccuggc gcucccucgc agaauugcuc ggucugcccg cagacggcug      180

cgcagacgga aacaucgccu ugaaagaauc cggcgccugc ucgugagcga gaaugugcug      240

accaaggaag agaugaaccu gcucuuuaaa caaaagaagc agaucgacgu cuggcagcug      300

agaguggaug cccuugagcg gaaacugaau aacgacgaac ucgcucgcgu ucugcuucac      360

cuggccaaga gacggggauu caaauccaau agaaagucug agcgcaacag caaagaaucc      420

ucugaguuuc ucaagaauau ugaggaaaac cagagcaucc uggcucaaua ucgguccgug      480

ggcgagauga uugucaagga uagcaaauuc gccuaccaua agagaaauaa acuggacucu      540

uauuccaaca ugaucgcgcg cgaugaccuc gaacgggaga uuaagcugau cuuugaaaaa      600

cagagagagu ucaauaaccc ggugugcaca gagcgccuug aagagaagua ccugaauauu      660

uggagcucuc agcggcccuu ugccuccaaa gaagauaucg agaagaaggu uggguucugu      720

acguuugaac cuaaagagaa gagagcuccc aaagccaccu auacauucca gagcuuuauc      780

gugugggagc acauuaacaa gcuccgccug gucucuccug acgaaacccg ggcucuuaca      840

gagaucgaaa gaaaccugcu cuacaaacaa gccuucucca agaauaagau gaccuauuac      900

gauauucgca aacugcugaa ccucagcgac gacauccauu uuaagggacu gcuuuacgau      960

cccaaauccu cucugaagca gauugagaau auccgguucc ucgaacugga cagcuaucac     1020

aaaaucagaa agugcauuga gaacguguac ggcaaggaug gcauccggau guucaaugag     1080

acagacauug auaccuuugg guaugcgcuu acgaucuuca aagacgauga agacauugug     1140

gccuaccugc agaacgagua uaucaccaag aauggaaaac gcgucuccaa ccucgcuaau     1200

aagguuuacg auaaaagccu gaucgacgaa cugcucaacc ugucuuuuuc caaguucgcc     1260

caccuuagca ugaaggcuau uagaaauauc cugccuuaua uggagcaggg cgagauuuac     1320

ucuaaagccu gugaacucgc cggguacaac uuuacaggac cgaagaaaaa ggagaaagcu     1380

cugcugcuuc ccgugauccc uaauauugcg aaccccgugg ucaugcgggc ccucacccaa     1440

agcagaaagg ugguuaaugc uaucauuaaa aaguauggcu ccccuguguc uauccauauc     1500

gaacuggccc gcgaucugag ccacuccuuc gacgagcgga agaaaauuca gaaggaccag     1560

acagaaaaca gaaagaagaa cgagaccgcc aucaaacaac ucauugagua cgaacugacg     1620

aagaauccca cagggcuuga uaucgucaag uuuaaacugu ggagcgagca gcagggacgc     1680

ugcauguauu cucucaagcc gauugaacug gagagacugc uugaaccugg cuacguggag     1740

guugaccaua uccuccccua uucccggagc cuggaugacu cuuacgcuaa caaagugcug     1800

guccucacca aggagaaucg cgaaaaaggc aaccacacac cuguggagua ucuggggcuu     1860

ggauccgaaa gauggaagaa guucgagaaa uuuguccugg ccaauaagca guucagcaaa     1920

aagaaaaagc aaaaccuccu gcggcugcgc uacgaagaga ccgaggaaaa ggaguuuaaa     1980

gaaagaaauc uuaacgauac ccgguacauc uccaaguucu uugcgaauuu cauuaaagag     2040

caccucaagu ucgcugacgg cgauggggga cagaaagugu auacaaucaa cggcaagauu     2100

acggcccauc ugcgcucuag augggacuuu aauaaaaacc gggaggaaag cgaucugcac     2160

caugccguug acgcugugau cgucgccugu gcuacccagg ggaugauuaa gaagaucaca     2220

gaguucuaca aagcccgcga acagaauaag gaguccgcga aaaagaaaga acccaucuuu     2280

ccccaaccuu ggccgcacuu cgccgaugag cucaaggcua gacugucuaa guuuccccag     2340

gagagcauug aagccuucgc ucuuggaaac uaugaccgga aaaagcugga gucccucaga     2400

ccuguguuug uuagccgcau gcccaaacgg ucugugaccg gcgccgcgca ucaggaaaca     2460

cugagacgcu gcgucggcau cgaugagcag uccgggaaga uucaaaccgc cgugaaaacg     2520

aagcugagcg acaucaaacu ugacaaagau ggacacuucc cuauguacca gaaggaaucu     2580

gacccccgga cauaugaggc uauuagacag cgccuccugg agcacaauaa cgauccgaaa     2640

aaggccuuuc aggaaccucu guacaaaccc aagaagaacg gcgagccugg gcccgugauc     2700

cggaccguua aaaucauuga caccaagaau aaagucgugc aucucgaugg auccaagaca     2760

guggcuuaua acagcaauau cgucagaacc gacguguucg aaaaagaugg caaguacuac     2820

uguguuccug uguauacaau ggacauuaug aaggggacgc ugcccaacaa agccaucgag     2880

gccaauaagc cguacucuga auggaaagag augaccgagg aauauacauu ucaauucagc     2940

cuuuuuccua acgaucuggu ccgcauugug cucccccggg agaagaccau caaaacaucc     3000

accaaugaag agauuaucau caaggacauu uucgcuuacu auaaaacgau cgauagcgcg     3060

accggcggac uggagcugau uucucacgac agaaacuucu cccuucgggg cguugggagc     3120

aagacacuga agcgcuuuga aaaauaccag guggaugucc ucggaaauau ccauaaggug     3180

aaaggcgaga agagaguugg gcuggccgcu cccaccaacc agaaaaaggg aaagacagug     3240

gacucccucc aaucugucag cgacuga                                         3267


<210>  3897
<211>  3270
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3897
augaccgccu ucaagcccaa cccucugaau uacauccucg gccuggacau ugggaucgcu       60

agcgugggau gggccauggu cgagauugau gacaaagaaa acccgguuag acuuaucgau      120

cugggcgugc ggguguuuga gcgcgcggaa augcccaaga caggggacuc ccucgcugcc      180

gccagacggc uggcucgcuc ugucagacgg cuuacgcgca gacgggccca ccgccugcuc      240

agagcucggc gccugcugaa acaugaggga gugcucaagc cugaagauuu cgacgagaau      300

ggccugguua aaccccuucc uuauauuccc aagcagccua gaaacacccc guggcaacug      360

cgggccgcgg cccucgaucg caagcugaca ccccuugagu gggcugccgu gcugcuccac      420

cuggucaaac auagagggua ccugagccag cggaagaaug aaggagagac cgacgauaaa      480

gaacucggcg cucugcuuaa gggcguggcc gacaacacac acgcgcugca ggccgggaau      540

uuuagaaccc cugcugagcu cgcccugaac aaauucgaga aggaauccgg acauauccgc      600

aaucagcggg gcgauuauuc ucacacauuu agcagaaagg accuucaagc ugagcugguu      660

cuccuguucg aaaaacagaa ggaguuuggg aaccccuacg uguccgauga ccugaaaaau      720

ggaaucgaaa accuccugau gacccagcgc ccugcccuuu cuggcgacgc cauucagaag      780

augcuggggc acugcacguu cgagcccgcu gagccgaaag cggccaagaa uaccuauaca      840

gcugaacggu uuaucuggcu caccaaacug aacaaucuuc gcauucugaa ccaaggaaac      900

gagcgcccuc ucacagaugc cgaacggacc acgcugaugg acgagcccua caaaaagagc      960

aaacugacau augcccaggc uagaaagcuc cuggaucuug acgauaccgc cuucuuuaaa     1020

ggccugcgcu acggcaagga caaugcggaa uccgcuacac ucauggagau gaaagccuac     1080

caugccauca gccgggcucu ggagaaggaa gggcugaagg auaaaaaguc uccucuuucc     1140

cucagccccg agcugcagga cgaaauugga accgccuucu cucuguuuaa aaccgaugag     1200

gaaaucacag gcagacucaa ggggugucug cagcccgaga uucuugacgc ucugcucaaa     1260

cacaucuccu ucgauaaguu cguccaaauc agccugaagg cccugcgccg gauugugccu     1320

cuuauggagc agggaaaaag auaugacgaa gcgugcgccg agaucuacgg cgaccaucac     1380

gggcggcgca acucugaaga gacgcucuau cugccgccca uuccugcuga ugagaucaga     1440

aaucccgugg uccugcgggc ccucucccag gcuagaaagg uuauuaacgg agugggccgc     1500

cgguacggca gcccugccag aauccacauc gaaaccgcgc gcgagguggg gaaauccuuu     1560

aaggacagaa aagaaauuga gaagcggcaa gaagagaauc gcaaggauag agagaaagcc     1620

gcugccgagu uccaggaaua uuuucccaac uucgucggag aaccgaaguc uaaagacauc     1680

cugaaacuuc ggcuguaccg ccagcagaau uguaagugcc ucuauagcgg caaggagauu     1740

aaccugggga gacugaauga aaaaggauac guggagaucg aucaugcccu uccuuuuucc     1800

cggacauggg acgauagcuu caacaauaag guucucgugc ugggcucuga gaaccaaaau     1860

aaggggaacc gcacccccua cgaauaucug aauggcaaag acaacuccag agaguggcag     1920

gaauuuaagg cucgggucga gacaagccgc uucccucacu cuaaaaagca gcggauucuc     1980

cugcagaaau uugaugaaga gggauucaag gagagaaacc uuaaugacac cagauacgug     2040

aaccgcuuuc uguguaaguu cguugccgau cauaugcaac ucacgggcaa agggaagcgg     2100

agaguguuug cuagcaaugg acagaucaca aaccugcugc gcggcuucug ggggcuucgg     2160

aaagucagag cggaaaauga ccgccaccau gcccucgaug cuguggucgu ggccugcucc     2220

accguugcca ugcagcagcg gaucaccaga uucgugcgcu auaaggagau gaacgcuuuu     2280

gacggaaaaa cagucgacaa ggaaaccggc gaggugcugc accaaaagac acacuucccc     2340

cagccuuggg aguuuuucgc ccaggaaguu augauucggg uguuugggaa acccgauggc     2400

aagccggagc uggaagaggc ggacacgccu gaaaaacuca gagcucugcu ugccgagaag     2460

cugucuagcc ggcccgaggc cguccaugaa uacguggcuc cccucuucgu gucccgcgcc     2520

ccuaauagaa aaaugagcgg acagggccac auggagacca ucaagucucc gaagcggcug     2580

gaugaaggga uuuccguugu ccgcgugccc cugacacaac uuaaacugaa ggaccucgag     2640

aaaaugguga acagagaacg ggagccuaag cuguaugagg cgcucaaagc uagacuggaa     2700

gcccauaagg augaccccgc caaggcuuuu gccgagccuu ucuacaagca ggauaaggcu     2760

ggaaaucgca cccagcaagu caaagccgug cggaucgaac agguucagaa gacaggcgug     2820

ugggucagaa accagaaugg gauugcggac aacgccacca uggugcgcgu ugauguguuu     2880

gagaaaggag gcaaguauua ccuugucccc aucuacagcu ggcaaguggc uaaagggauu     2940

cugccugaca aggccaucau cgcuuucaag gaugaagagg aguggcagcu cauugacgau     3000

ucuuuuaauu ucaaauuucc ccugcacccg aacgaccugg uugaagugau cacgaagaaa     3060

gcccggauuu ucggcuauuu cgccuccugu cacagaggaa ccggcaauau caacauucgc     3120

auccaugacc uugauaacaa ggucgggaaa aauggaaucc uggagggcau uggggugaag     3180

acagcucuca gcuuucagaa guaccagauc gacgaacugg gaaaagagau ucggccuugc     3240

agacucaaga aaagaccccc ugugagauga                                      3270


<210>  3898
<211>  3270
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3898
augaacguga gauacaagau cggccuggac auugggauca ccagcgucgg augggccguu       60

gugaaucucg auauuccccg gaucgaggac cugggcgugc gcauuuucga uagagcugaa      120

aacccucaga caggggaguc ccuugcccug ccgcggcgcc ucgcgagauc ugcucggcgc      180

agacugcggc gcagaaaaca ccggcuugaa cgcaucagac ggcugcucau ccgcgagggc      240

auucugauga aggaagaacu ggacaaacuc uuugaggaaa gacaugagau cgaugucugg      300

caacugcggg uggaagcccu ugacagaaag cugaauagcg augagcucgc ccgcguucug      360

cuucaccugg cuaaacggag aggauucaag uccaaccgca agucugagca uaauagccgg      420

gaaaacucca cgaugcucaa acacauugag gaaaauagag ccauccuguc uagcuaucgc      480

accguggcug agaugauugu caaggacccc aaauuugccc ugcauaagcg gaacaaaggg      540

gaacacuaca caaauaccau cgcgagagau gaccucgagc gcgagauuca gcugaucuuc      600

acaaagcagc gggaauuugg aaacaugaau ugcaccaagg aguucgaaaa cgaguauauc      660

gccauuuggg cuucccagag accuuuugcc agcaaagaug acaucgaaaa gaaagugggc      720

uucuguacau uugagcccaa ggagaaacgc gcuccuaagg ccaccuacac guuccaaucu      780

uuuauugcgu gggaacacau caauaaacuu cggcugguuu cccccgccgg caccagaggg      840

cucacagaug aggaacggcg ccugcuuuau gagcaggcuu uccagaagaa caagauuacc      900

uaccaugaca ucagaacacu gcuccggcug ccugacgaua ccuauuuuaa aggagugguc      960

uacgacagaa auaugccgcu gaagcagaac gaaacgaucc gcuuccucga gcuggaugcc     1020

uaccaccaaa uucggaaagu gaucgacaag gcuuauggca aagggaaguc uagcuccuuc     1080

cuucccauug auuuugacac auucggauac gcccugaccc ucuuuaagga ugacgccgau     1140

gugagaagcu aucugcagaa cgaguacgaa cagaauggca aaccuaugcc caaccuugcu     1200

aauaagacau augacaacga gcugaucgag gagcuccuga aucugucuuu caccaaauuu     1260

gggcaucucu cccugaaggc gcuucacaac auucugccuu acauggagca gggagaaguc     1320

uauagcuccg ccugcgagcg cgcuggcuac aaauucaccg gccccagaaa gaagcagaaa     1380

acaaugcucc ugccgaauau cccuucuauc gccaaccccg uugugaugcg ggcccugacg     1440

caagcucgca agguggucaa ugccauuauc aaaaaguacg ggagcccugu guccauucac     1500

aucgaacuug cgagagaucu cagccagacc uuugacgagc ggcgcaaaac aaagagagaa     1560

caagacgaga accggaagaa aaaugagacc gcuauucgcc agcugaugga auauggacug     1620

acacucaacc ccaccggcca ugauaucguu aaguucaaac uguggucuga gcagaauggg     1680

agaugugccu acucccuuca gcccauugaa aucgagcggc ugcucgaacc uggauauacg     1740

gagguggacc acgucauccc guacagccgc ucucuggaug acagcuauac aaacaaggug     1800

cugguucuua ccaaagagaa uagagaaaag ggcaaccgga cccccgccga guaccucggg     1860

gugggaacag aaagauggca ucaauuugag accuucgucc ugacaaacaa acaguuuucc     1920

aagaagaaac gcgaucggcu gcucagacug cacuaugacg aaaaugagga gacggaguuc     1980

aagaaccgca aucuuaacga uacccgguac auuucuagau uuuucgcuaa uuucauccgc     2040

gaacaucuga aauuugccga aagcgacgau aagcagaaag uguacacagu caacggccgg     2100

auuaccgcuc accucagauc ccgcugggag uucaauaaga accgggagga aagcgaccug     2160

caccaugccg uggaugcggc caucguugcu ugcacaaccc cuucugacau ugccaagauc     2220

acggcuuuuu aucagagacg ggagcaaaau aaagaacugg ccaagaaaac cgagccccac     2280

uucccucagc ccuggccgca uuuugcggau gaacuucgcg ccagacucuc caagcacccu     2340

aaagagagca ucaaggcucu gaaccugggc aauuacgaca acgagaagcu cgaaucucug     2400

caccccgugu ucgucucccg gaugccuaaa cgcagcguga caggggccgc ucaucaggag     2460

acccuuagac gguauguugg aauugacgaa agauccggca agauccagac aguggucaaa     2520

accaagcugu cugagauuaa acucgaugcc agcgggcacu uucccaugua cggaaaggaa     2580

uccgacccuc gcacguauga ggccauccgg caaagacugc uggagcauaa uaacgauccc     2640

aagaaggcuu uccaggaacc gcuuuacaaa ccuaaaaaga auggcgagcc cgggcccauu     2700

auccgcacag ugaaaaucau ugacaccaag aaccaggugg uuccucucaa cgauggcaaa     2760

acagucgcgu auaauucuaa caucgugcgg guggacgucu uugaaaagga uggaaaguac     2820

uacugugugc cgguuuauac cauggacauu augaaaggcg ugcugcccaa uaaggccauc     2880

gagccuaaca aacccuacag cgaauggaag gagaugaccg aggauuauac auuccaguuu     2940

ucccuguacc cuaaugaccu cauuagaauc gaacugcccc gcgagaaagc uauccggacg     3000

agcaccgggg aagagauuaa gaucaaggau uucuuugccu auuacaaaac aauucacucu     3060

ggaaccggcg ggauggagcu uaucucccau gaccgcagcu ucucucuguc cggagucggc     3120

agcagaacac ucaagagauu cgaaaaauau caaguggaug uucuggggaa cauuuacaag     3180

gugcggggcg agaaacgcgu cggacuggcc ucuagcgcuc acuccaagac cggcaagacg     3240

gugagaccuc uucagagcac acgggacuga                                      3270


<210>  3899
<211>  3273
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3899
augagauggc ggcugggccu cgacaugggg gccggaagcc ugggcuggug cgcugugggg       60

cuugaugacc agggauaccc cgauuccauc cuggccgcgg gcucucacau uuucggggac      120

ggacgcgcuc cuagcagagg cgaucagcag ggcaccccgc ucggggucga gcggcgcgcc      180

gccaaggcua ugagacggcg cagagaccgg uuucugggac gccaggccaa acuucuggcu      240

agacucacac uggccggccu guuccccgcg gauccuguuc gggccaaggc ucucgaacug      300

cuugaccccu augugcugcg cgccagagcu cucgaugagg cccugccucc ccaucagcuu      360

gggcgggcgu uuuuccaccu gaaccaacgc agaggauuua aauccaaucg gaagacggac      420

agaggcgugg augacgaagc cgggaaaauc gcuauuggaa ucgaucgccu ccgggccgcu      480

auugccgccg cugaggcgag aaccaugggc gaauuucugc auaagcgccg gcagucugcc      540

ccugacguca accagauccc gagcgugaga acacgccugc ggcccgagac cggcgaggaa      600

gcuaaggggg acggauacga uuuuuauccu ggcagagagc ucguugaaga ggaguucgac      660

gccaucuggg augcccaggc uccccaccau ccuucccugc uuacacccga caaucgcgcc      720

cugcuccacg aagcgauuuu cuaccaacgg ccgcugaaag cuccuaagau cgggaaaugu      780

acccuucuga acaaugagcc cagacucccu aaggcccacc cccuguucca gcgccggaga      840

cugcucgaag aggugaacgc ccugcggauu guccgccccg gagaaguggc uacaaaacuu      900

accugcgagc agagagaucu gcucaugcug aagcugaagg acaaagccaa gguuucuuuu      960

gagacgcugc ggagcaaagu gcucaagcug gaaccugagg cuagauucaa uaaagaaggc     1020

gagaaccgca ccgaacuggu cggggaugag gugugugccc agauguccgc gaagacacgg     1080

uuuggaaaag ccugggcuca uuucaccgac gagaagagau gggccauccu cagccgccug     1140

auugaagagg aagaugagga aagacuugac acauggcugc ggaccgagca cggccucgau     1200

gcugagcaag cccgcgcggu ggccggcgcu agacugccgg ccggguucgg acgguuuggc     1260

gaaacggcua cacgcagacu gaucgccgcc cuucaggcug aggucauugu uuauucugaa     1320

gcgguggugg ccgcugggcu cggacaucac uccgacaugc ggaccggcga gguccuggcc     1380

gagcugcccu acuaugggau gacacucgaa cgccaccuuc uuccuggcac cggagacaag     1440

gccgaucccg aggaacugag aaucggccgg cucaccaauc cuacagugca uaucgcucug     1500

aaccgccuga gacggauugu uaauagacuu aucaaaacgu acgggcgccc cgagcagauu     1560

gccaucgaac ucggacggga gcugaagcug accgacgagg auaaacaaag acucagccgc     1620

cagaacaauc ggaacagagc ggacgcugaa gcccgcucua agaaacuggc cgagcuuggc     1680

caggcugaua auggggccaa ccgggcgaga cugaagcucu gggaggaacu gaaccgcgaa     1740

aaugcucugg accggagaug cauuuacucc ggacagcaaa ucagcaucgc caugcuuuuc     1800

ucugaugccg uggaggucga ccacauucuc ccguuugaug cuacacugga cgauuccaac     1860

aauaaccgga uccugugucu ccgcgagugg aauagacgga agcgcaaaca gagcccuuau     1920

gaagccuggg gccauaccga cuacuggccc gauauugcug ccagagcggc ccggcugccu     1980

aaggagaaaa gauggcgcuu cgaacccgac gcuaugcagc gguuuuccgc cgaggacaga     2040

uucaucgcuc gccaccuugu ggauacacag uauaugucuc ggcuggccag agaauaccuc     2100

gaggcccugu auccugagaa gggggaaggc agcggacaug uuuggguguc ccccggccgc     2160

cugaccgagc uuauucggag aaaacucggg cugaacgaac ugcucccgga ccacaauuuu     2220

gcuggaguca agaaccgccu ggaucacaga caucacacga ucgacgcguu cguggucgcc     2280

gugacagauc ggagccuucu gcaaggcauc ucucgcgcuu ccgcccucga gggggaggaa     2340

ggaagagagc ggguuauugu gccugaaccc ugggacggcu uucgcgaggc ccugagaacc     2400

acccugaaua caaucaccgu cuggcaucgg caggaucacg ggacagcuag caaggccggc     2460

cuugcgcagg gaagagacca gacggcuggc aaacugcaca acgauaccgc cuacgggcuc     2520

acaggacgcc aagacaccaa gggcaaugau cuggugguuu cucggaaacc ccucaugagc     2580

cugaagccug ccgacauuga accggugccc gaugucgcuc cuaucacaaa aggggugaga     2640

gaccgcgacc uucggaaggc ccuggcgcau uucacccagg cuacggaggg aaagggcuuu     2700

cuccaggccc uggagagauu cacccggcug gggccccuuc acuuucgcgg cauuagacgg     2760

gugcgccuga uccagccucu cgccguuauu cccaucaaag gaccuggcgg gguccccuau     2820

aagggauacc ugggcgggga aaacuucaga ugcgaugugu gggagaugcc ggacggagcu     2880

uggaaaccug aagugacauc cauguuccaa gcccaucagc ccggcuggac cucugcuauc     2940

cgggccgcgu guccuacagc ccgcaagguc cucagccugc acaaaagaga uaugauugcu     3000

aucgagcggg aagagggcgu gcgcacccuu augguugugg cccagauuug guccaauggg     3060

caagucacgc ugguggagcc ccaugaagcu ggagaccuca acaagagaaa uaaaagcgag     3120

gaugacccgu uuggcuacuu caugccuaca gccgggggac ugaagcggcu gaaggccaga     3180

cagguucgcg uggaugaacu uggccggguc caggaccccg gguuuccugc uagaaaagcg     3240

cgccggagaa cccgccccaa gcaaguggau uga                                  3273


<210>  3900
<211>  3273
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3900
auggacugga gacugggccu cgaucugggg gccaacagcc uuggaugggc ugcccugcgg       60

cucgaccgcg cgggcgaucc caucgggauu cuggcuaugg gauccagaau cuucucugac      120

ggccgggauc cuaagagcgg ggccucccuu gccguggacc gcagagcugc ccgggcuaug      180

cgcagacggc gcgauagauu uaaacagcgg caagccgcgc ugcucaagua ccugauucgc      240

gacggacugu ucccggccga ucaggcugac agacagcggc ucgccgauga ggaccccuau      300

gcucugcgcg ccagagcgcu ugaugaagaa cugucucucu uugaaaucgg ccgggcccug      360

uuccaccuua aucagagacg cggcuuucaa agcaaccgga aagcugacag agggcaggac      420

gaugaggccg gaaagauugc ugucggcguu gaccgccuga aucagcagau ggaggagcaa      480

ggggcccgga cccucggaga guuccugcau cagagacgcc gggccgcuac agaugcgaac      540

cacaucccuu ccgugagaac gcgccugcgg cccgaaaccg gcgagggggc caaaggagac      600

ggcuacgauu uuuauccuuc uagagcucuc aucgccgaag aguucgagga aauuugggcc      660

gcucaggcuc cccauuaccc ugacacacug acccagcaag ugcacgaucg ccuuuaugag      720

aucauuuuuc aucagcggcc gcugagagcg ccccaggucg gccaaugcac acucguggcu      780

ggggaaaccc ggcugccuaa ggcccacccc cuuuuccaga agcgcagacu gcucgaggaa      840

aucaaugccc ugaugauugu ucggccugga gagaucgcuc agagacugac acccgaggaa      900

cgcgacaccc ucuaccugaa acuuaaggau aaacggacgg ugagcuuugc cacccugaga      960

aagacacuca aacuggacuc cgcugcccgc uucaacaagg agagcgaaaa uagaaccgag     1020

cuuaagggcg augaagucgc ggccgagcug ggggcuaaaa cacggcucgg aacccgcugg     1080

cagcaccugu cuggcgacca acagugggag gugguucaua gacugauggc ccucgaaucc     1140

caggcugacg aggccgcguu ucgggauugg auuagccaga cguaugaccu gcuuccggaa     1200

caagccgcug ccgugcuggg ggcucgccuc ccucagggac acggcagauu cggccccaca     1260

gccaccacaa cccugaucga uucucugcuu caugaccgga ccgaggaugg gcgcgucgug     1320

guguacuccg aagccgucca gagaagcgga cucggccacc acucugaccu gcggcaugau     1380

cgcgacgaug aggacguuac agagcugccu uacuaugggg uggcucucga aagacacauc     1440

augcccggaa cggcggagcc caccgauccu gaagagaugc ggauuggcag acugacaaac     1500

ccgaccgugc auaucgggcu uaaucagcug cgccacgucg ugaaccggcu cauuagagcc     1560

uacggacccc cuaaggagau cgcuauugaa cuggcccgcg agcugaaacu uuccgaagac     1620

gagaagaaaa agcggaauag cgaaaacaga gauaaucgcc ucgccgcuga gggccggucc     1680

aaaaagcugg aggaaaucag acaacgcgac acaggcgcca accgggcgcu gcucaagcug     1740

ugggaggaac uuaaucccgg gaacguucug gacagacggu guaucuauac cggagagcag     1800

auuucuaucg auaugcuguu cagcgaccgc guggaagucg aucauauucu cccuuuugcu     1860

cagacgcugg acgauuccaa cggcaauaaa cuucucugcc ugagagaggc caaccgggag     1920

aagcgcagac ggagccccua cgaagccugg gggcacacag acagauggcc ggauaucgcu     1980

gagcgcgccu cucggcugcc uaaacccaag agauggcgcu ucgaaccuga cgcgaugcag     2040

cgguuugcua ccgaggaugg auuccucgcc agacaccugg uggacaccca auaucuuggc     2100

cgccuggccc gggaauaccu cuccuggcug uaucccggga gaggcgaggg aagcggccau     2160

guuugggugu cuccugggcg ccugacagag auggucagac ggaaacuugg acucaaugau     2220

cugcugcccg accacaacuu uggcggggga gcugaucagc cgaagaaucg ccucgaccau     2280

agacaccacg ccauugacgc ucuggugguc gccaucgugg aucggggcau gcuucaggcg     2340

aucgcccgcg cuagcgggca agacggcucc gaaggaagag agcggauuca uaucccugcc     2400

cccugggaug gcuucagaga cgaucugcgc guugcuguga acgggauuau cgucucucac     2460

cggcccgacc auggaaccgu ggccaaggcc ggccucgcua gagggcgcga ucagacagcg     2520

ggacggcugc acaaugacac ggccuacggc cugaccgggg aaacagaugc uaaaggcaac     2580

agcauuguug ugagacggau cccucuuucc gccauuaaga aaccggccga caucgcucgc     2640

gucagagauc cccugcuccg gaaugcccug gcggaguuua ccaagggacu cgacggcaaa     2700

gccuucgagg cccgcaucgg gagauuucau gaacugggac cucuucugua ccggggcauu     2760

cgcagagugc gggugguuga gacacucacc gucaucccca uucgcgacgc cgaagggaga     2820

gcuuauaagg gauacaaggg cgauagcaac uaucgguacg acguguggga gaugccugau     2880

ggcaaaugga gaugucaggu ggucucuaug uucgacgccc accaguccac ggaacaagcg     2940

cgcccccacc cugcugcccg gaagauccug agccugcaga gaaaugauau ucuugccauc     3000

gagcgcaccg agggggaccg ggaacuggug agaguuguga aauuuucuga gaagcaguuc     3060

gcugucgcuc ccccgcauga aggaggcucc cucaaaacac gcgaugggaa cgccgacgau     3120

ccuuuucggu aucuguaccc cagcccuaau acccucaagg gauggagagc ccgccaggug     3180

cggaucgacg agcugggcag aguucuugau cccggguucc cggcgcgcaa guccgugcgg     3240

agaacacgcc cuagagccgc ucccgucgac uga                                  3273


<210>  3901
<211>  3276
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3901
auguacagca accuccucuu cuccuuugac aucggcacca auucuauugg guggugcgug       60

uucgcccugg augaggcugg agaacccuau agaaucgucg accuuggcgc ccggauuuac      120

gcggaugggc gcgacccuca gagcaagaca ucccuggcug uugccagacg ggaggcccgc      180

gcuaugucua gacggcgcga uagauaucuc cggcgcagaa aagccacgcu gcggaccaug      240

aucgaauacg gacuuaugcc gggcgacaag gcugggcaag aggccgugcu gcgcgaaaca      300

ggagauagag agggcggcga cgagagcuuu aaccccuaug cgcuccgggc ccgcgcucug      360

uccgaaaaac ugccucccgc cuacauuggg agagugcugu uccaccuggg acagcggaga      420

ggcuuuaagu cuaaucgcaa aaccgaucgg aaggacaacg auaaggggaa aaucgcucuu      480

ggaaucgacg agcugacagc cgcgaugcau agaagcggcu ccccuacccu cggggccugg      540

cuggcuaugc gccgggccga aggacacagc gucagacuuc gcgcuggcuc ugagguguuc      600

gaugccgagg gcuaugccuu uuaccccgaa cggucccugc ucgaggacga guucagacag      660

auuugggacg cucaggcggu ucaucacccu gauauccuga caccggaacg ccgggcccau      720

cuguuuagag ugauguucua ccaacgcccc cucaagaaac cucuggucgg gcgguguagc      780

uuuaaucccg cugaauuuag acuuucucgg gcccacccuc uguuccagga guuccgccuc      840

uauaaggaga uuaacgaccu ggaagugguu cuucccgauc agucccacag aaaacugacc      900

cucgacgagc ggaaugcccu gguggcuaag cugaagacga gcagaaaagc cucuuucucc      960

gcgcuccgca agacccugaa acuuacaccg gaucuggucu uuaacaagga aagcgaggcu     1020

cggaaagaac uccugggaga cgagaucuac uccgugcuug ccgaugccaa gauguucggc     1080

gcuagauggg gaggguuuuc ucgcgccaga cagugggaga uuaucacccg gcugaaagaa     1140

gaggaagacc cugcuagccu cuccagcugg cugaagucug aguucggccu ggaugacgaa     1200

cgcgugacag ccauugcgaa ugccucccuc cccgaugggu auggaagacu gggcgagacc     1260

gcucuugcca gcaugcugga ggaacucaag acggcuguca ucccugaguc ugaagccgcg     1320

cggcugugcg gcuacgacca uagcaaccug gccaaagagc aagacgaggg gcuugauaca     1380

cuccccgaau aucaggagau ccuggaacgc cacauucccc cuggaaccgg cgacccggau     1440

gacaucuacg aucugagaaa ggggcggauu acaaauccca ccguucauau cggacucaac     1500

cagcugcgca gaguggugaa ugcucuuauu caccgguuug gccgcccuca gagaaucguc     1560

guggagcugg cccgggaccu ccaacugucc gaaaaacaga aggaugacgu uaacagacgc     1620

aucgcuaaaa auacccggga ggccgagggg agaucucaga agcugauuga aaugggccaa     1680

cuugauacag gauauaaccg ccuccugcug aagcucuggg aggaacugaa ccuugacaaa     1740

cccgaggauc ggguguguau cuacagcggc aagccuauug ggaucgccau gcuguucucc     1800

ggagaagucg acguggauca cauucucccc uggagcaaaa cgcuggacga cucucaggcu     1860

aauaagcugc uuugccucaa auccgcgaac agacagaagc gcaaucggac cccgagcgau     1920

guuccugagu ggagagaccg cuacgaugag gugcuggccc gggcugccag acugccccgg     1980

aacaagcgcu ggagauuugc cgcugacgcc augcagcaau ucgaagcgga gggcggguuu     2040

cucgcucggc agcugacaga uacccaguau cuuucucgca uggcccugga auaccucucc     2100

gcccuguauc cuagcgagga agcugacaaa uccggagagc ugagacagcg gaagagaguc     2160

cauguggucc ccggccgccu uacagagcuc cugcggagaa auugggggcu gaacacccuc     2220

cugccugauc acaaucuugg cgaaauggcc caagagaaaa accgccggga ccauagacac     2280

caugcgaucg augcugccgu gaucggaacg acaucucgca gccugcucca gcggaugucc     2340

ggcgccgcug ccagacugga cgauguugcu uucgacgauc uggugcgcac cgucgugaag     2400

gacaaucccc cguggccugg guuuagagag gaacuucugg ccgcgauugg acggguuacc     2460

gugucucaca aacccgacca cggcacaguc agccgcgccg cuuacgccca ggggaaggga     2520

cagaccgcug gcaagcucca uaacgauaca gccuaugggc ugacgggccu cguggacgcc     2580

aaaggaagcc cccugguggu uagacggaag ccuuucaugg cucuugaggu caaagauauc     2640

gcguccauuc gcgacaccga acugcaaucu gcccucuacg gcgcuaucgg gucccugaca     2700

gagaagaaag cccugcagga agcccuuauu agauuucggg auacccaccc gcaguucaag     2760

ggaaucagac gcgugcgggu gcuggagaca cucagcguca uucccaucgu ggacagagau     2820

ggcaaggcuu auaaagggua caagggagac gccaauuacc gcuaugaagu uugggagacc     2880

cuggauggca aauggcauac ggaggugguc ucuauguucg acgcgcacca gccugggugg     2940

caaucccccu uucacaagca gaacccugcu gcccggagag ugcucaaacu gcagcaaaau     3000

gauaugguug ccuacgaaca ucccgcugac ggcuauacca ucgcccgggu ggucaaguuc     3060

agcaacgaca aacgcauuua cuuugcgucu cacagagaga gcggaucccu uaaggcucgg     3120

gaugccgaca agggcgaucc uuucagcuau uuugccaaag cuaauaacgg gcugcgcgac     3180

aucaagugua gacaggugcg gauugaugcc gcuggacgca ucuucgaccc cggcccgcag     3240

gauagagaaa cacggcaggc caacaaaggg guuuga                               3276


<210>  3902
<211>  3279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3902
auggccuaca ccaugggcau cgacgugggg auugcuagcu gcggaugggc caucgucgau       60

cuggagagac agcggauuau cgacauuggc guucgcacau ucgaaaaggc ggagaacccc      120

aaaaaugggg aagcucucgc cgugccuaga cgggaggccc gcuccucuag acggcgccug      180

agacggaaga aacaccgcau cgaaagacuu aaguauaugu uugugcggaa cggacuggcu      240

gucgauaucc aacaucucga gcagacgcug cgcagccaga augagauuga cguguggcag      300

cuuagaguug auggccugga ccggaugcuc acccaaaaag aauggcugcg cgugcugauc      360

caccucgccc agagacgggg guuccagucc aacagaaaga cagauggauc uagcgaggac      420

ggccaggucc uggugaaugu uaccgaaaac gaucgccuua uggaggagaa ggacuaccgg      480

acaguggcug aaaugauggu caaagaugag aaguuuuccg accauaaaag aaauaagaac      540

ggcaauuauc acgggguggu gucucgcgcc agccugcucg ucgaaauuca uacccuguuc      600

gagucccaac ggcagcacca caacuuucuu gcgagcaaag auuucgaacu ggaguacauc      660

aauauuuggu cuucccagag accgguugcc acaaaggacc agaucgagaa gaugauugga      720

accuguacgu uucuccccaa agaaaagcgc gcuccuaaag ccagcuggca uuuccaauau      780

uuuaugcugc ugcagaccau caaccacauc cggauuacaa augcucaggg caccagaccc      840

cucaacaagg aggaaaucga gcaaguggug aauauggccc ugacaaaauc uaaggucucc      900

uaccaugaca uucgcaaagu gcuugaucug agcgaagagu aucaguucgu ugggcucgac      960

uacggaaagg aggaugaaaa gaaaaaggug gagucuaaag aaaccaucau uaagcuggac     1020

gauuaccaca aacuuaacaa gaucuuuaac gaggucgaac uggcgaaggg cgagacgugg     1080

gaggccgugg acuaugauac aguugcuuac gcccucaccu ucuuuaaaga cgaugaagac     1140

auccgggauu aucugcagaa uaaguacaaa gacuccaaga acagacuggu gaaaaauguc     1200

gcuaacaagg aguauacaaa ugaacucauu gagaagguga gcacccuguc cuuccgcaaa     1260

gucgggcacc uuucucugaa ggcccuccgg aaaaucauuc cuuuccugga gcagggaaug     1320

accuacgaca aggccugcca agcugcgggc uuuaacuucc agggcaucag caaaaagaag     1380

agauccgugg uucuucccgu gauugaucag aucagcaauc cugucgugaa ccgggcccug     1440

acacagacgc gcaaaguuau caaugcucuc auuaagaaau augggucucc ggaaaccauc     1500

cauauugaga cagccagaga acuguccaag cgguuugacg agagaaagaa caucaccaag     1560

gauuacaaag aaaaucgcga caagaacgag cgggccaaga aacaccugag cgagcucgga     1620

auuaucaauc ccacaggccu ggauauugug aaguacaaac uuugguguga acaacagggg     1680

agaugcaugu auucuaacca gccuaucagc uucgagcgcc ugagagaauc cggauacacc     1740

gaggucgacc auaucauucc cuauucucgg agcaugaaug auuccuacaa caaccgcgug     1800

cucgugauga cgagagaaaa ucgggaaaag agcaaccaga caccuuauga guacaugggc     1860

aaugacaccc aacgcuggga ugaguuugag cagagaguua ccacaaaccc ccagaucaaa     1920

aaggaaaagc ggcagaaucu gcugcuuaaa ggguucacca accgcagaga gcucgaaaug     1980

cuggagcgga aucugaacga cacacgccac auuacgaagu aucucucuca uuuuaucucc     2040

accaaucugg aguucagccc gucugauaaa aagaaaaagg ucgugaacac auccggaaga     2100

auuaccagcc accuucgguc cagauggggc cuggaaaaga aucgcgagca aaacgaccuc     2160

caccaugcua uggaugccgu ggucaucgcg gugacaucug acagcuuuau ccagcagguu     2220

accaauuacu acaaacggaa ggaaagacgc gagcugaacg gcgacgauaa auucccucug     2280

cccuggaagu uuuuccggga ggaagugauu gcuagacuuu ccccuaaucc caaagagcaa     2340

aucgaagccc uccccaacca cuuuuauucu gaggacgaac uggccgaucu gcagccuauu     2400

uucgucagcc gcaugccgaa gcgguccauc acgggggagg cucaucaggc ccaguucaga     2460

cgcgugguug gaaagaccaa agagggcaag aacauuacag cuaaaaagac cgcccucgug     2520

gacaucagcu acgauaaaaa uggggacuuu aacauguaug gacgggaaac agaucccgcg     2580

accuacgagg ccaucaagga aagauaucug gaguucggcg ggaaugcuaa aaaggccuuu     2640

ucuacggacc uucacaagcc uaaaaaggau ggcacaaaag gaccccugau uaaguccguc     2700

cggaucaugg aaaacaaaac ccucgugcac ccuguuaaua agggcaaggg gguggucuac     2760

aacagcucua uugugcgcac agacguguuc caaagaaaag agaaguauua ccugcugccc     2820

gucuacguua ccgaugugac caaaggaaaa cuuccgaaua aagugaucgc ugccaagaag     2880

ggcuaugacg auuggauuga ggucgacgac uccuuuacau uccucuacag ccuguauccu     2940

aacgaucuga ucuuuauccg gcagaauccc aaaaagaaaa uuucucucaa gaaacgcauc     3000

gaaagccauu ccauuagcga cucuaaggag gugcaggaaa uccugacgua cuauaagggg     3060

guugauucca gcaccgcggc cauugaguuc aucacacacg acggauccua cuaugcuaaa     3120

ggcguggggg uccagaaccu ugauuguuuu gaaaaguacc aaguggacau ucugggcaau     3180

uacucuaaag uuaagggaga gaaaagacuc gagcuggaaa ccagcgauaa caaucauaag     3240

ggcaaggacg ugaacuccau caaaucuaca agccgguga                            3279


<210>  3903
<211>  3279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3903
augaaguaca gcaucggccu ggacauuggg aucgccuccg ugggaugguc ugucauuaac       60

aaagauaagg agagaaucga agacaugggc guucggauuu uccagaaagc ugagaauccc      120

aaggauggga gcucccucgc cucuagccgc agagaaaaac ggggaucccg cagacggaac      180

cgcagaaaga agcaccggcu ggaccgcauc aaaaauaucc uuugcgaguc uggccuggug      240

aagaaaaacg aaauugagaa gaucuauaaa aaugcguacc ucaagagccc uugggagcug      300

agagcuaagu cccuugaagc caaaauuagc aacaaggaga ucgcccaaau ucugcuccau      360

aucgcuaaac ggcgcggguu uaagucuuuc agaaaaaccg aucggaaugc cgacgauaca      420

ggaaagcugc uguccggcau ucaggaaaac aaaaagauca uggaggagaa gggcuaucuc      480

acgaucgggg acaugguggc uaaagauccg aaguuuaaua cccacguccg caacaaagcc      540

ggaagcuacc uguucucuuu uuccagaaaa cuucuggaag acgaggugcg gaaaauucag      600

gcgaagcaga aggaacucgg caauacacau uucaccgaug acguucugga gaaauauauc      660

gaaguguuua acagccaaag aaauuucgau gaggggcccu cuaagccuuc ccccuacuau      720

agcgagauug gacagaucgc caaaaugauu ggcaacugua cauuugaauc cucugagaag      780

cgcaccgcua aaaauacaug gagcggggaa cgguucgucu uucuucagaa gcugaacaac      840

uucagaaucg ugggacucuc cggcaagcgc ccucugaccg aggaagaacg ggacaucguu      900

gagaaagaag uguaccugaa gaaagagguc agauacgaaa agcuccgcaa aauucuguau      960

cuuaaggagg aggagcgguu uggcgaccug aauuacagca aggaugagaa acaggacaag     1020

aaaacggaaa agaccaaauu caucucucuc auugggaacu auacaaucaa gaaacugaau     1080

cuuuccgaga agcucaagag cgaaauugag gaggauaaau cuaagcugga caaaaucauc     1140

gaaauucuga ccuucaacaa gagcgauaaa acaaucgagu ccaaucugaa gaagcucgaa     1200

cugucuagag aggacauuga aauccuucug agcgaggagu uuuccggaac ccucaaccug     1260

agccuuaaag ccauuaagaa aauccugccc uaccucgaaa agggccuguc uuauaaugag     1320

gcuugcgaaa aagccgauua cgacuauaag aacaauggga uuaaguucaa acgcggagag     1380

cugcucccug ugguggauaa ggaccugauc gcgaacccgg ucguucuucg ggccaucucc     1440

caaacgagaa aaguggugaa ugcuauuauc cgcaaguacg gcacacccca caccauucau     1500

gucgaagugg cccgggaucu ggcuaaaagc uacgacgaca gacagacaau cauuaaggag     1560

aacaagaaac gggagcucga aaaugagaag accaaaaagu uuaucucuga agaguucggg     1620

aucaaaaacg uuaagggaaa acugcugcuu aaguaucgcc ucuaccagga acaggagggc     1680

agaugugccu auucccggaa ggagcugagc cuguccgaag ugauucucga ugagucuaug     1740

accgacaucg aucacauuau cccuuacagc agauccaugg acgauucuua uagcaauaaa     1800

guccuggugc uuuccggcga aaaccgcaag aaaagcaacc ugcuccccaa ggaguacuuu     1860

gaccggcaag ggagagauug ggacacauuc guucugaaug ugaaagccau gaagauucac     1920

ccucgcaaga aaucuaaccu gcuuaaggag aaauuuacga gagaagauaa uaaggacugg     1980

aaaucccggg cucucaacga uacccgcuau aucagcagau ucgucgcgaa uuaccuggag     2040

aacgcccugg aauaccggga cgauucuccc aagaagcgcg uguuuaugau cccgggacag     2100

cucacagcuc agcugagagc ccgguggcgc cuuaauaaag ucagagagaa cggcgaccug     2160

caucacgccc ucgacgcugc cgugguugcg gugaccgauc aaaaggcuau uaauaacauc     2220

uccaauauua gccgguauaa agaacugaag aacugcaaag acgucauccc uucuauugag     2280

uaccaugccg augaggaaac aggggaggug uauuucgaag agguuaagga cacccgcuuu     2340

cccaugccuu ggagcggauu cgaucuggaa cuucagaaga gacucgaguc cgagaauccc     2400

cgggaagagu uuuacaaccu gcugagcgac aaaagauauc ucggcugguu caauuacgaa     2460

gagggguuca ucgaaaagcu gcgccccgug uuugucucuc ggaugccuaa cagaggcgug     2520

aaaggacagg cccaccagga gacgauccgc uccagcaaga aaauuuccaa ccaaaucgcu     2580

gugucuaaga agccgcuuaa uagcauuaaa cugaaggauc ucgagaaaau gcagggccgg     2640

gacacagaua gaaagcugua ugaagcccug aaaaaccgcc uugaggaaua cgacgauaag     2700

cccgagaagg cuuucgccga gccuuuuuac aagcccacca auuccgggaa gcggggaccu     2760

cucguuagag gcaucaaagu cgaagagaag cagaacgugg ggguguaugu caauggaggc     2820

caggcgucua acgggagcau ggugcgcauu gacguuuucc ggaaaaaugg caaguuuuac     2880

accgugccca ucuaugucca ccaaacacug cugaaagaac ucccgaacag agccauuaau     2940

ggaaagccuu acaaggauug ggaccugauc gacggcuccu ucgaguuucu uuauucuuuc     3000

uaccccaacg aucugaucga aauugaguuu gggaaaagca aguccaucaa aaaugacaac     3060

aagcucacca aaacagagau uccugaagug aaucugagcg agguucuggg auauuaccgg     3120

ggcauggaua cgucuaccgg ggcugccaca aucgacaccc aggauggaaa gauucagaug     3180

cgcaucggca ucaagacagu gaaaaacauu aagaaauacc aggucgacgu gcuugggaau     3240

guuuauaagg ugaaaagaga aaagcggcaa accuucuga                            3279


<210>  3904
<211>  3285
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3904
augaaaagca cccuggccau cgcuuccauu ucuagccucg gcugggugau cgagcugggg       60

gaaaacagaa uuaugcggcu uggacuggac aucggcucca caucuauugg gugguggauc      120

uacgagacga ccgauggaca gaucacaagc gucauugacg gcgggguucg caucuucucc      180

gauggaagag accccaaauc uaagggcagc cucgccgugg aucggcgcgu ggcgagagcu      240

caacggcgca gacgggaccg cuaucugaga cggaaagccg cccuuaugaa gcgcauggcu      300

gaagucggcc ugaugccuuc cgauccgaaa caggccaagc agcucgaggc ucuggacccc      360

uacgaacuga gagcccgggg gcucgaugag acccuggagc uuacagaacu gggacgcgcg      420

cucuuucacc ugaaucagag acggggcuuc aagagcaaca gaaaaaccga cgccggggau      480

aaugagucug gaaagauuau cgacgcuaca gcccgccuug auugggcuau gcgggaccau      540

agagcccgca ccuauggcga auuucugcac augcggagac aaaaagcgga cacgccucgc      600

accacacccg ccgugcggac cagacucaca auugcugccc gcgauggggc ugagaaggag      660

gaauccggau acgacuucua uccugaucgg cagcaucuga gagacgaguu ugaugcccug      720

ugggaagccc aggcugagua ccacggcguu cucaccgaag accugcgcga ugagaucggc      780

acgauuaucu uccaucagcg gccccuuaaa acaccuaagg uggggaagug ccuguuuacc      840

aacgaggaaa gagucgcgag cgcccacccg cucaaucaac ggcgcauccu gguggagaca      900

guuaacgcuc uuagaguggc ccagcuggga gaagucgccc gggcucucac ccuggaggaa      960

agagacacca uucugcacgc ccucgauaau aaagcgcaua caaagucucu guccggcaug     1020

agcaucaaac uuaaggcucu ggggaaagug auuaagcucc gcucuggaca guauuucacg     1080

cuggagaccg ccaaccggga cgccaucgcu ugugaucccg ugagagccuc ccuuagcaaa     1140

ccugaccgca uuggcuccua cuggucuaca cuggaccaca augcucagug ggaggucguu     1200

aacagaaucc gggccgugca aagcgaugcg gaccaucgcg aacucguggc cuggcugguc     1260

ucccagcaca gacuggcugc cgagcacgcu gaaggggccg cguacgcucc ccucccugau     1320

ggauauagcc ggcugggcau caccgccaca aagcgcauuc uugccgcucu ggaggccaau     1380

gugauccccu acucuaaggc cguugaaucc ugcggcuggc aucacagcga cgagagaacc     1440

ggggaggugc ucgaucggcu gccguauuac ggagaaauuc uggaccgcca ugucaucccu     1500

ggcacguaug augacucuga ugacgauauu acaagauuug ggcggaucac caaccccacc     1560

gugcacaucg gacuuaauca gcuccgcaga cugguuaacc ggauuaucga ggugcauggc     1620

aaaccugacg aaauugucgu ggagcuggcu agagaucuca agcugagcca agaccagaaa     1680

gcggacgucg agcgcacaau caagaaaaau acccaggccg cuauucggag aggggaaaaa     1740

cuugaggaaa ugggcgugcc caacacagga ggcaaccgca ugguucugcg gcucugggag     1800

gaucugaaug acgaugacgu gaugaagaga uucugucccu acacggggca gcgcaucucc     1860

gccggaaugc uguuugaugg cucuugcgac gucgaucaca uucuuccuuu cagccggacc     1920

cucgacgaug gguuuacaaa caagacccug ugucugaagg aagccaauag aaagaagcgc     1980

aacuaugcuc cgcacgaggc cgcggagaaa ggacuccugg accgggaugc uauggacucc     2040

guggccaagu accuucccga uaauaaggcc uggagauucg cuccugacgc caucgaacgg     2100

uucgagggcg aacgcgacuu uacagcgaga gcucugaaag auacccaaua ccucagccgg     2160

cuggcccgca cguaucugga cgcccuuuac gaugggggcg acgauaaguc ucauguuugg     2220

gugguccccg gaagacucac cgagaugcug cggagacacu ggggccugaa cgggcucgaa     2280

gcucuggccg acuccgauga gaaaacagug aagcgcaaaa aucggaccga ccauagacac     2340

cacgcuaucg augccgcggu gguugccgcu acagaccgca gccuuauuca gaagaucucu     2400

aagauugccg cuuccgauga gcaggccgga gccgaagaca cccugaaaag cacgccuccc     2460

ccgugggacu ccuuccggga ugacaucgcu gcgcagcucc aaaacauuac agucucucau     2520

agagccgauc acggccgcac cgacagccug gcuaaggggc ggggagagga uacaaccucc     2580

ggcgcccugc augaagccac cgcucuuggg aucaucgacg agacacacgu gacggugcgc     2640

auuccuaucg aaucucucaa aagcggccau cugauugagg gauccagaaa uggcuauauc     2700

agagaucccu accugcggga ccagcucacc cuggccacaa agaacgggag cgauaaagac     2760

uuuaccacag uccuucagaa guucuuugau cgcucuaaaa gauauggagg cacccggcgc     2820

gugagaauua ucaagacgcu gcaggacaca gcgcggaucu ccguuccuca caaugcuccc     2880

auuaaggccu acaaaggggg aagcaaccac gccuucgaga ucuggagacu cccuaauggc     2940

aagauugaau cucaagugau caccaccugg gagcaucaca accugacaga acccaaacgc     3000

ccgcauccug cugccaagcg gauuaucaga cugcacaaaa ccgauauggu cgcgaucgag     3060

cgcgacggga agacagugau uugcuuuguu cagaagacgg accccuccaa uggcgcuuuc     3120

cuugugcccc auuuugaggc caacgccgau ggacggaaaa gaaagaacga accugucgag     3180

ugguaucaga ugagcgcuaa uucucugauc aaagccgacc uccggcgcgu gggcguugau     3240

gaaaugggga gagugcggga cccgggaccc ccucccauuc gcuga                     3285


<210>  3905
<211>  3285
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3905
augaaguaca gagugggcuu cgaccugggg accaacagcc ucggaugggc cgucguucgg       60

cugaaugcuu ccggcgagcc cgaucgcauc gaaguggccg ggucuagaau uuuuagcgac      120

ggacgggagc cuaaauccgg cgcgucucuu gcuggggccc gcagagaugc ccgggcuaug      180

cgcagacggc gcgacagauu caagcagcgg caagccgcuc ugaucaaaua ucucacacag      240

gccggauuuu ucccggcgga ugaaagcgag cgcaaggccc uguccgcucu ugaccccuac      300

gaacugagag cccgggcucu cgaugaggcc cugccucugg cgcaccucgg ccgcgcccug      360

uuucaucuua accagagacg gggcuucaaa ucuaauagaa agacggaccg cgggaagaac      420

gaugacaccg gaaaaauucg gaucggcgug gcuagacugu augagcagau gcgcgaugcc      480

gacgcucgga cacucgggga auuucugcac aagagacgcg ccgccgcuag cgaucccaau      540

gcgauuccuc auguccggac cagaaucgug acacuugccg cugaggacgg aaaagccucc      600

gacguuuacg auuucuaucc cgaccgccaa acccuggaag aggaguuuga agccaucugg      660

gaggcucaga caagccacca uccugaagcc cucaccgagg aacggagagc gcaguucuac      720

gagauuaucu uucaccagcg cccgcugaag caacccaaaa uuggcuucug cacgcugcuc      780

ccucccgaga agcggcugcc uaaggcucac ccccuuuuuc agagacggcg ccugcucgaa      840

gaggugaacg cccugagaau cguccuuccg gggaaagaaa cccgggcccu ggagaaggcu      900

gaaagagaug cccuccuguc uaaacugaau cagcgcaaga aagcugccua ugagucccuc      960

cggaaggcgc ugaaacuuga cccugaugcc agauucaaca aggagagcga caaucgcgaa     1020

gauaugauug gagacgagau cgcugccgaa auggcucaua agucuagauu cggcaaagug     1080

uggcugggca uguccgccga ggcgcaggcc caaguuauug aacggcuccg cgggcuggag     1140

agcgaugcug acgccgauga cuuugaggcu uggcuuagag acgaucacgg acuggcccag     1200

gaacaggcca aggcugugac agcggccaac cuccccgagg gcuacgggcg guucggagaa     1260

accgcuacac gcaaacugau cgccgcccug gagcaagagg ucaucgugua cucugaagcu     1320

gugcagagag ccggccucgg gcaucacucc gaccugcggg auggacaggu cuggaccgac     1380

aagaaaggca auccugcgcu ucccuauuac ggcgcuguuc uggagcgcca cauugugccc     1440

gggacggccg auccuaacga aacagacgau gcccucagaa ucggacggcu gaccaauccg     1500

acagugcaua uuggccugaa ccagcuucgc agagucguga augcucucau gcggagauau     1560

gggcaacccc aggagaucgc cauugaacug gcgcgcgagc ugaagcucag cgacgaucgg     1620

aagaaagaga ucaacaccag aaaccgcgaa aaucggaagg cugccgagaa aagaucccag     1680

aagcugcgca acaucggaca ggccgacaaa ggcgggaauc gggcccuucu gaagcucugg     1740

gaggaacuga acccugaaaa uguucuggau agacgcugug uguacaccgg ccgggucauu     1800

ucuaucgaca ugcuuuuuag cggagcugcu gagguggauc acauucuccc cuucuccguu     1860

agccuggacg acucuaacgg caauagaauc cugugccucc gggaggugaa ccgcauuaag     1920

agacaacgga caccuuggga agcccgcgau gaccugagag cgcauuuugg gcccgaugcc     1980

ggaugggagg cuaucgccgc ucgggccgcc agacuuccgc gcgaaaaacg guggagauuc     2040

gagccugacg cugcgaaucg cuuugaugcc cagggcgggu uccuggcucg gcaccucacg     2100

gacacccagc aucuguccag acuggcccgc agcuaucuuu cugcccucua ccccgaaaca     2160

ggagagggca gcgggcacgu cugggugucc ccuggccggc ugaccgagau gcugagacgc     2220

gcuuggucuc ucaaccacca ucugcccgau cacaauaucg ccggagcggc uaaccagccu     2280

aagaauagaa aagaccaucg gcaccacgcc auugaugcca ucgucguggc ucuugccgac     2340

cgcgcgaugc ugaacagaau ugcucgggaa agcggcaagg aggggcauga agccgccgga     2400

cgccucguug cuuccauccc cgagccgugg gagggcuuua gcgaugaagu ggggagagcc     2460

guccgggcua uuacaguguc ucacagaccu gaccauggaa ccgccuccaa agcgggccgc     2520

gccaaggaua aggacgcuac ggccgggcgg cugcacaaug agacagcuua uggccugacc     2580

ggagaaaaag acgccagagg ccuugauauc guugugaccc gcaagccccu cagcgccuuc     2640

aaaaagucug cugaccugga ugcgauucgg gacgaugacc ugaaacaaaa gcucaaagcc     2700

ugggcugccg ggaaggaggg aggcgccuuu gaacaggcua ugagauccuu cggggauccc     2760

gagcggggac cucgcagcca uccgggccug agacggaucc gcgucacaga gccccuuucc     2820

gugaucccua uuagagaccg ggaugggcgc cccuacaagg ccuacaaagg cgacucuaac     2880

uauagauuug auaucuggga acugaagacc ggaaaaugga aggacgaggu gguuagcaug     2940

uucgaagcgc accggccuaa cuggacaucc cccauucgcg cuggcaaucc uacggccaga     3000

aaaguccucu cugugcggca gaacgacgug aucgccauug agagagaaaa ugggcccgag     3060

cugaugcgcg ucgugaaguu cagcgcugga ucccuggccc uugcgccgcc ucaggaggcc     3120

ggcagccuga aggcucggga ugccgacaaa aacgaucccu uuaaguacgu uuaugggucu     3180

ccuuccagcc uccaaagaug gaaagcucgc caggugcgga ucgacgaacu gggaagaguc     3240

uucgaucccg gcuuugccgu gcgcucucgg agauccagcg aguga                     3285


<210>  3906
<211>  3291
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3906
augaagaaca ucuucagcuu ugacauuggc accaauucca ucgggugggc cgugcuggcu       60

cucgauggaa acggcgaccc cgucagaauu gccgaugcgg ggguucggau cuucucugac      120

ggacgcgauc cuaaaagcaa ggcuucccug gccgaggcca gacggauggc ucgcgccaug      180

ucuagacggc gcgacagaac acuucggcgc aaaagagcua cgcugcggac ccucgccgaa      240

auuggccuga ugccggauac aagcuccgcg cgccaggccc aacuuagaga gaccucugac      300

cccaagagcg gguccggaca gcugacacgg aauccuuacg cucuccgcgc ccuggcucug      360

gaagagaaac ucccccuguu ucacaucggc agagcccuuu aucaucuggg ccagcggaga      420

ggguuccaga gcaaccgcaa gaccgaucgg aaggacaaug auaaaggaaa gaucgcgcuc      480

ggcauugaag aacugcacgc cgcuaugaga caagacgggg cccagacaua cggacagugg      540

cuugcucgcc ggcaugcccu gggccaggcc gugagacucc gcucuggggc ucuggaucug      600

gagggagaag gcuaugcguu uuacccugag cggaaacucc uggaagacga guuccacaga      660

aucugggagg cccaagcucg ccauuauccc gacauucuua ccccugaacg gagagaucac      720

cuguuuacgg ugauguucuu ucagcgcccg cucaagaaac ccaaggucgg caagugcucc      780

uucaacaauc aggaggaacg gcugagcaaa gcccacccuc uuuuucagga guucagacug      840

uacaaggaag ugaacgagcu cgagaucguu gaacccgacc ugacccauaa aaagcugaca      900

cucgaucaac ggaaugcccu ggugaaaaag cugcaggucu cucgcgaggc uuccuuugcc      960

gcgcugagaa aagugcucaa gcugcggccu gaccagagac uuaacaagga aagcgagucu     1020

cgcaccaaac ugaaggggaa ugaaguuuau gcugugcucu ccgauaaaac acgguucgga     1080

cccagauggg gcaccuucag ccaagccgag cagugggcca uuaucgagcg ccugaaggaa     1140

gaggaagacc cggggagacu guuugcuugg cuccgguccg ccuucggaau ugagggcgaa     1200

caggcugccg auaucgcgaa cguccaucug ccugaggggc acggacgccu uggcaaaacg     1260

gcccuggagg cuaugcucga ggagcugaag gccgaaguga ucacagaggc ugaggccgcg     1320

aagagaugug gcuacgacca cgcccggcuu gggggaggcu cugaugaagg ggagcccgaa     1380

cuuccucccu accaggagau ucuccaacac cgcauucccc cuggaaccaa uaacccggac     1440

gaucccuaug acauugaaaa aggcagauuu acaaauccua ccgugcauau cgcucugaac     1500

cagcugcggc acgucaugaa cacccucauu cgcagauacg ggaagcccgc ccagaucgcu     1560

guugagcugg cccgggaccu uaaacugagc gagcgccaga aggaagaggu gaauagaaca     1620

aucgcccgga acacgagaga ugcugaacgc cggggcaaaa cccucgagga aaauagaauc     1680

ccuaacacag gauauaaucg cacccugcug cggcuuuggg aggagcucaa ccccggcaag     1740

guggaagaca gaugcuguau cuacacaggg aagccgauuu ccaucagcau gcuguucucu     1800

ggagaggucg auauugacca uauccugccu uggucccgca cccucgauga cagccacggc     1860

aauaaacugc uuugccugcg ggaagcgaac agagugaagc gcaaucgggc ccccgcugau     1920

guuacggagu ggagagacaa cuaugaugaa auccucucuc gggccgcccg ccugccucaa     1980

aauaaaagag accgguuugc ucccgaugcc auggagcgcu ucgagaaggc ugggggauuu     2040

cuggcgagac agcuuacaga cacccaguac cucagccgga uggcccugga auaucuggcc     2100

ugucucuucc cugcugagga aauggauggc uuuggggagc ugagacgcca ucgguacauu     2160

aacgugugcc ccggcagacu uaccgagaug cugcgccgga auuggggacu caacuccauc     2220

cugucugaca gccagcuggg cgaaacagcc caagagaaaa auagaaagga ccaccgccac     2280

caugcgauug augcuaucgu cauuggggug accggacggu cccuucucaa cagaaucagc     2340

uacgccgccg gccgccugga agcucagcag cuggagacac ucguuaagga agccgcugcc     2400

acgaacaaac cgugggacgg guucagagag gauguggcga aggucguggg aggcauuauc     2460

gucucucaca aaccugacca ugggaccgcc ggccggaagg cuaaagaucc cgcccgccag     2520

caaacagcug gaagacugca caaugacacc gccuauggcc uuacagggga gaccgaugcc     2580

cggggaaaca cgaucguggu ucgcagaaag cccuuucugu cccucaccag caaagacauu     2640

ccuucuaucc gggaugaagc ucugagagcg gugcuggagc agcaccuucg cggcauuccg     2700

gacgggaagg ccuuccaggc ugcccuggau gccuuucgga agcaaaaagg auuccaucac     2760

uaccauggca ucagacacgu ccgccucaca gaaccccugu ccgugauugg gaucaaggac     2820

cgggauggca aaguuuauaa gggauacaaa ggcgacgcua aucagagaua ugacgugugg     2880

gagacccucg augggaagug gcaugccgaa gucgugagca uguucgacgc gcaccagccu     2940

ggauggcagu cccgguuuca cgcugagaac cccacagccc gcagagugcu gcggcuucaa     3000

caggaugaca ugguugcccu gaaugcuccu gauggcgggc gccuccuggu cagaguggug     3060

aaguuccggc agaacggaca gaucaccuuu gccggccaua augagucugg caaccugaaa     3120

gcgcgcgacg cugccagcaa ugaacaagau cccuucaagu acauuuccag aauggccucu     3180

agccuuaaag acugugggug ccggcagguc cgcaucgaug cuacgggaca gguguuugac     3240

ccuggccccc aggauagagc cucccggcug caaaagcaga gcaaaagaug a              3291


<210>  3907
<211>  3294
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3907
augaagaaac agccccaaag auacauccug ggccucgacc uggggauugc cagcguggga       60

ugggcucuua uccuggucga uggcgccgag aacccuguug ggcuccugga cugcggagug      120

cggaccuucg aacgcgcgga ggugccgaag acaggcgauu cccuugcucu ggccagacgg      180

caggcccgcu cugucagacg gcucauucgc agacgggcuc accgccugcu gagacuccgg      240

cgccugcuua aaagagaagg gauccugagc gccgcugacu uuaauuccga uggacucccc      300

aaccugccua uugacgccug gcagcuucgg guggcgggcc uggagcgcaa gcucacgaau      360

caggaauggg ccgcuguucu gcugcaucuc gugaaacaca gaggcuaucu gucucaacgg      420

aaaagcgaga cccagacaga gaacaaggaa cuugggagac ugcucgccgg agucgcugaa      480

aauuccaaac ugcuuucuga aagcuccgag caguaccgca cccccgccga gcuggcggug      540

aagcuguucg ccgaacagag cggccaucug cggaacaaag ggggagagua uacacacacc      600

uuuaauagac uggaucucca aaaggaacug caucuucugu ucgagcgcca guacgcucuc      660

ggcaacccuu uugccucugc ugaacugcag uccaaaguug acgagcuucu gaugacacag      720

cggagcgccc uccaagggga ggccgugcug aagaugcugg gaaaguguac cuucgaaccc      780

ucugaguaua aagcugcgaa gaauacguac uccgccgaaa gauuuaucug gcucaccaaa      840

cugaacaauc uucgcauccu guauaacggc gaggaacggg cucucgauga gggcgacaga      900

aagaaacugc uggaugagcc uuacaagaaa gccaaacuua cauacaagca gguccgcagc      960

auucucaauc ugccggaauc ugccguguuc aaagggcugc gguauggaaa ggacuccgau     1020

ggccucaaag cugagaacgg gacccugaug gaaaugaagg ccuaccacaa aaucagaaca     1080

auucuugaca aggcgggacu gaagggcgag ugggaaaauc gcauccggaa ccccgagauu     1140

cucgacaaaa ucgggaccgc uuuuagccug uauaagacgg augaggacau ucaggccgcc     1200

cuggcugauc uuagacuccc ugaaaacgug cuggacgccc ugcucgcugg acugaauuuc     1260

gauaaauuua uccaacuuuc ccugaaggcc cuccggaaca uccugccccu gauggagcgc     1320

ggcauuagau acgacgaagc gugcaaagag gucuauggcg aucaccgggc ccuugcugag     1380

ucuaagaauc agguucuccu gccucccauc ccggccgacg aaauuagaaa cccuguggug     1440

cugcgcacac ucacccaggc ucggaagguc aucaaugcca uuaucagacg cuacgggagc     1500

ccccagagag ugcauaucga gacagcgcgg gaacugggaa aauccaagca agagcgcgau     1560

aaaauugaca gaaagaacaa agaaaaucag aacgaucgga aucgccuugc cagcgacauc     1620

agaaagcacu ucaacuuuga gccuaccccc aaggacauuc ugaaacuccg gcuguaugag     1680

cagcagcaug gcaagugucu guacucugau cuucgcgaag agaaagggga augccucuac     1740

accggaaaug uuaucgagcu gcacagacug uucgaagacg gcuaugugga gauugaucac     1800

gcucuccccc ugucccggac augggacaac agccuuaaua acaagguccu ggugcucaaa     1860

ucuagcaauc aaaacaaggg gaacaaaacg ccuuacgagu ggcuggaugg cgccaauaac     1920

ucccgcagau ggcgggaguu ccaggcuaga guucagggau gucauuuucc guaugugaag     1980

aagcagcgca uccaaaccgc caaacuggac ucuaaggccg agcagggcuu ccuugaacgg     2040

aaucugaacg auacaagaua caucagcaaa uuuaugugcc aguucauuac cgcuaaucuc     2100

cgccuggcgg agacauccaa cgcccucaug gaagaggucg cuggggagaa ucccuauugg     2160

aaggaacgga aaagacagcg cguguuugcc ccuagcggau cuaucauugc ccugcuucgg     2220

ggccuguggg ggcucaagaa ggucagagag gacaacgauc gccaccaugc ucuggacgcc     2280

aucgugguug cguguuccac cgugagcaug caacagaaaa uuacgaaggc uaugcagcgg     2340

agagaaacac uggagaccgu cgauucugag accggagaag ugaaaacacg gaucccccaa     2400

ccuugggacu ucuuucgcca ggagguuaug auuagagugu ucuccgauag cccccgggag     2460

gaacuugccg aaaagcucaa uucccgcccg gaggcccucc acgacuuugu cucuccucug     2520

uucgugagca gagcucccaa ccggaaaaug accggccagg ggcaucucga gacagugaag     2580

uccgccaaga gacugucuga aaauaucagc guuguccgca aaccucuuac ggcgcugaag     2640

cucaaagaca ucgagaacau ugugggcuac cccgaucggg aaccugcucu guaucaggcc     2700

cugagagagc gccuucuggc ccacaaggac gaucccguga aggcuuuugc cgacaagccg     2760

uucuacaagc cugcuaaaga uggaggccaa gggucccucg ucaaggccgu gcggaucauu     2820

gaaaauacca aacacggacu gaagaucaga aacggcauua cagacaccaa uggggauaug     2880

guucgcacag acguguacca gaaaaccgau aagaaaggaa agcggcagua uuuuaucguc     2940

cccguguacg cguggcaggu ugagaagaac gagcucccca cgguggacug caaaggcuau     3000

gaaaucgaug acaacuacga guucuguuuc agccuguaua aguacgaucu uauugaaauc     3060

caaaauccua acgacauuaa ucaggagcug agagccuauu acaucaacug cgacucuucc     3120

gaugggcgcu uuuggcuggc uuaccaugac aaaagcgcca aggaacagca guucagaauu     3180

ucuacccaaa aucagccgau caucaaaaag uaucaggucg auacacucgg caaggagauu     3240

cggcugugua aagcugagaa gcgcaccgcc cuuaacggaa agaagaaaaa guga           3294


<210>  3908
<211>  3294
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3908
augggcaagg gggacauccu guucagcuuu gauauuggaa ccaacuccau cggcuggugc       60

guguucgccc ucucugacaa uggggauccc uguagaaucc uggacgcuaa cgcccggauc      120

uuugcggaug gacgcgagcc uaaaagcggc aauucccuug cugaagggag acgggccgcc      180

cgcgcuaugg ccagacggcg cgacagauac aagcggcgca gaaaagcugu ccugcggaca      240

cucacggagu auggacugau gccggccgau cccaaggcgc gccaggcccu uguugcuaaa      300

accggcgaca gaggcccuaa gucugaagag gcccuggcug auguguacag ccuccgggcc      360

cgcgcgcuga uggaaaagcu gccccuccac cauauuggga gagcccuguu ccaccuuaac      420

caacggagag gauuuaaauc caaucgcaag acagaccgga aaucuaacga gaagggcgcu      480

aucgagaccg gggugaauag acugcucgaa gagaugcgcg auacaggagc cccuacccug      540

ggcgcuuauc uugccaaacg gagaagcgac gggcgccccg uccgggugag agccggagcu      600

uccgaacagg aggcggagga uggcaagccu gccaagacag cuuacgacuu cuauccgguu      660

cgccggcugc ucgaagagga auuugacgcc aucugggaga aacaguacag cuaucauccc      720

gaagugcuga ccgaggagag acgcggccac cuguuccggg ucauguuuca ucagagaccu      780

cucaagcccg ccauuguggg gcggugcucu uucaacuccg cugaaacgcg ccuggccaaa      840

gcgcacccuc uuuuucaaga guucagacug uacaaggaag uuaaugagcu cgaagugguc      900

augcccgauc agagccaccg gaaacugacc cuuccggaga gagaccgccu ggugcucaag      960

cugcggggac agagaaaagc uacauacgcc gcccugcgca aggagcucag acugcgggau     1020

cuucgcuuua acaaggaaac cgagaauaga acagacauga ugggcgauga agugaccgcu     1080

gcccuggcug acaaagccgu cuucgggaac cgguggucug gauucuccac ggaugagaag     1140

uggcgcaucg uugaaagacu ccgggaggag gaagacccuu uugcgcugca ugaguggcuu     1200

gugagcgaac acggccuguc ugaugccgag gcugagcagg ccgcuaaagu gcaucucccc     1260

gacggguaug gacgccuggg cccuacagcc gucuccacca ugcuggaaga acucaaggcg     1320

gaugugauua cagaagccga cgcugccaga cuggcuggcu acgaccacag ccuugcccgg     1380

gccgaugagg acgggcugga ucgccucccc aaauaucaag aaguucugga gagacggauu     1440

cccccuggaa ccaaugaccc ggaugacccc uacgauauug cuaagggcag aaucaccaac     1500

ccuacagugc acauugcgcu gaaucaguuc cgccaugucg ugaacgcccu ugcucggaga     1560

uuugggaagc ccgaccagau cgccaucgag cucgcccgcg aacugaaacu gaacgaucgg     1620

cagaaggacg agguuaauag agugauugga cgcaacacgc gggcugccgc gcucagaucc     1680

caaaaacugc gcgauaucgg ccagaaggac aauggguaua accgggcccu ucugaaacuc     1740

ugggaggagc ugaauccuga uaagcccgaa gacagagucu guguguacac cggccggccg     1800

auuugcaucu cuaugcuguu cagcggagag gucgacgugg aucacauucu uccuuggucc     1860

cgcacaaugg acgauagcca ggcuaacaga auccucugug uuguggaggc caaucggguc     1920

aaggcuaacc gcgcccccgc ggaaguggcu gaguggggcg ggagauauga cgaaauccug     1980

gagcgggccg ccagacugcc ucgcaauaaa cgguggagau uugcucgcga ugccauggaa     2040

aaguacaaag agaacgaggg auuccucgcu cggcaacuga ccgacacaca guaccuuucu     2100

agacuggccc gcgaauaugc ggaggcccuc uaccccaccg aggaaccuga ucggcugggc     2160

gagcugagac gcagaaauca uguucgggug auucccgggc gccuuacgga aaugcucaga     2220

cggggauggg gccugaacgc ucugcucccg gaccacaauu uuggggccgg ccaggcuaag     2280

aaccgcaaag aucauagaca ccacgccauc gacgccgcug ucgugggagu gacaacccgg     2340

ggccugcuuc agagaauuuc caccgaggcg gggcugcgcg aaaagcucgc ccuggaggaa     2400

acaaucgcug auauuccuau gcccuggccc ggauuccggg aggagcugaa ggccgccauc     2460

gacagagcug uugucagcca uaaaccugau cacggcaccc uuccgaagcc cggggaacau     2520

ggacgcacag ccggccaacu ccacaaugac acggcguaug ggaucaccgg cgagacagac     2580

gcucggggaa acccuauugu ggugagacgg aaacccuuuc ugucucugac cgauaaggac     2640

aucccuagca uucgcgauac agaacucaga ggccgggucu acuccgccac cucugggcug     2700

agcggacgcg agcuuuccgc cgcucuggac uauuucagaa aaacggauaa gcaguuucgg     2760

ggcauccgcc acgugagagu uaccgaaagc cucucuguga uucccauccg ggaccgcgau     2820

gggaacauuu acaaaggaua uaagggcgac gccaauuuca gauacgaugu cugggagcug     2880

ccugacggga aguggaaagc ggaugugguu acaauguuug acgcucaucg gcccgacaga     2940

gccgccgagg aacgccggcc gcacccugcu gccagaaagg ugcugucccu uaaacagaac     3000

gauauggucg cuugcgagca ucccaaggac ggcuacacca ucggacgcgu gguuaaauuc     3060

aauacagccg gccagaucua uuucgcggcc cacaacgaag ggggaagccu gaaggcucgg     3120

gaugccgaca gagcugaucc uuuuaaguac uucucuaaau ccgccagcgg ccucaaggag     3180

auugccgcuc gccaagugcg gaucgacgcg accgggcagg uccuggaucc cggaccgcag     3240

gacagagaau cccgccucgc ccggaauaaa agacuggagc gcgagcgggc uuga           3294


<210>  3909
<211>  3303
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3909
augaaguaca ccaucggccu ggacauuggg acaagcuccg ugggaugggg cgucaucgau       60

acggagagaa accggauuca ggaccucggg guucgccugu ucgaaucugc cgagaaucaa      120

aaagauggag aaagccuugc uaagcccaga cgggacgccc gcuccaugag acggcgccug      180

gcgagacggg gcucucgccu cgagagagug aaaagcaucu uugaaaaggc uaaauuccug      240

acccgggggc agaugggaau uacacacgcc cgcccuaacg auccguauag acuucgggcc      300

gagggccugg accgccuccu guccaaugag gaucuguuua ucgcugugua ccaucucgcc      360

aagagacggg gcuauaaguc uaacagaaag aauguccugg aaggggacgg agaggaugug      420

cgcgaaggca aggcuguucu ugacggggug gcccagaaca aaaagcugau ggcguuccgg      480

ggauacagaa ccgucggcga gauguuuuuc aaagauagcg uguuuacaga ggccaagcgc      540

aauaaggcug gguccuauaa acacugcguu cuccgggaaa ugcuggagga cgaacuugga      600

aagauccugc augcccagag aagccucggc aacacccugg ugacagauuc uuucauuucc      660

aaacugcucg agaagaaaac cggcgucuuu guguaccaac gccccuucgc uagcgggaau      720

cagauccuga aacuuguggg accuuguacg uuugaacccg aggagauucg ggccgcgaaa      780

gccaccuauu cuuuccagua cuuuaacguc cugcagagac ucaauaaccu gacaaucaag      840

aauaccgaca caggcaccga acgcaaacuu acguccgagg aacgggcuaa gaugauugcc      900

uucaucuuca gccacaagga cguucaauac gcucagauua gacuggagcu caaacugucu      960

gauguggacc gcuuuaacau ggugaauuau uucguccagc ggaaggaacu ggagacaaaa     1020

accgccgagg aacucagacg ggagaucgaa aagaagacaa aauuuccuuc ccugagcgau     1080

uacuauacca uccgcaagac cguguccgag auugacucug ccuucuggau ccagauugaa     1140

aaacaacaug auguuaucga cgcuauuggg gagaucuuua cacuuuacaa gacggaugag     1200

gacaucauug cgcagcugaa caccagaauc cccagcauuc cucggccggu ggucuccgcc     1260

cuccugggac uuagcuucac aaaauuuggc caccugucuc ucaaggcucu gagaaagauc     1320

auuccccacc uggaaaacgg ggauaccuau gacguggccu gcgccaaagc ugauccuaag     1380

uacacagacc gcgccaaaac ccgggcgaga aagcuccgcc cccuggacaa agaggaucau     1440

uccaucacga auccuguugc uagacgggcc aucgcccaga caauuaaggu ggucaacgcu     1500

aucauugaca aguaugauag ccccucugaa gugcacaucg agcuuggacg cgaccuggcc     1560

aaaaauuuca aggagagaau gaaaauucaa aaggaacaga aacagaacca ggagggcaau     1620

gaacgggcua ucgccaagau ucaugagcuc uacaaacugg aaaacccgcg cggccaagau     1680

aucaucaagu uuagacugug gaaugagcag aacgggcagu guauguacag cggagagcgg     1740

auugacgaaa uccgccuuuu cgauaauggc uauaccgaga uugaccacau cauuccuuuu     1800

uccagaucuu ucaacgauag cuacuccaau aagguccucg ugcugaaaau gcggaaccag     1860

gaaaagauga auaaaacccc cuaugaguac augggaggag acgcggauac auggcaacgc     1920

uuugaaaacc ugguuaauag caugcacaac aucggcccua gaaagaaaca gaaccuccug     1980

cuuaagaagu auguggccga cgaucugacc cuccggacac ugaaugacac gagauucauc     2040

gcucgcuaca ugaaaaacua uauugagaau acccugcuuu ucacagaggg gccccagaag     2100

cagcgggucu acaccgugaa cggccaagcc acagcuuacc ucagaaaacg cuggguucug     2160

cauaaggacc ggaccgaaaa uaacagacac caugcccagg augcgguggu cguggccgug     2220

gcuacgcccu cucugguuca gggaaucaug cgccacgcca aaaugggcga gauugucgac     2280

uaucuccauu cccagaaguu ugcuccuaau gugaccgaua ucgaaacagg gaagccguuc     2340

gagagcgccg acauucaauc ugccaaagaa cggaucaucg cuaagcacga gcacagacug     2400

cgcauucccg agccuuggga aggauuuucc gaugagcuug acgcgcggau gggcgaugac     2460

guggucgaaa aacugagccu cagaaagucc aacauccaug gguacaaaac cacaucugau     2520

uucagccaga uucaccccau cuuugugucc cggaugccua agcgcaagac cacggggaga     2580

gcccaucagg agacacugcg gucuccccgc agauucaaag aguuucaaga aagcguugug     2640

cggaccccgc uguccagcau uaaucuuaag aaacucgagg aaaugucugg caagaacucc     2700

gacaaacugc uguaugauau ccucagaaag cgccugcuug cucacgggga cgauccuaaa     2760

aaggccuuca cagagcccgu ccacaagccu cggaaagacg gcacccaggg accccuggug     2820

agaagcauca agaugguuuc ugaaggccag cgcuccggga uucucgugaa uaacgagcag     2880

gcccuggucg accaagauag caugauccgg auugacaucu uuucuaaacc uaauaagaaa     2940

ggaaaggaug aguacuauau uauccccgug uacgcuuauc auuucgccca gaaggaacug     3000

ccgaacaaag uuauuaccgc gaaggcugag gccgacugga gaacaaucga ugacacguuu     3060

gccuucgaau uuagccuuua cccuaaugau cucgugaaac ugaccaaggg cgacgauauc     3120

cgcuucgggu acuauuccgg augcaaccgg gcucuggccg cgauucacau ccucccccau     3180

gaccagaaca ugccccuggu caauaacggc auuaaaacac uugauaagau cgacaaguac     3240

gugguggaca uucuggggga guaucacaaa gucggcaagg aaaccagaca gcaaauccag     3300

uga                                                                   3303


<210>  3910
<211>  3303
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3910
augggcgcca ccaucgugac agacauuagc agauccgcuu ucgccuuuga uaucgggacg       60

aagucuauug gaagcgcggu ccugcggcuc gacgaucgcg gcgagcccau cgggauuauc      120

aacgcugccg uuagaaucua cgccgacgga cgggaaccua aauccggcac cucucuggcu      180

gaggggcgca gacuugcccg gggaaugagc cgcagacggg aucgcuauaa gagacggcgc      240

agaacacugc ucaauacccu gacagaguuc ggccuuaugc cgcccaccga cgaggcucgg      300

aaagcccugc ucgcggaaac aaacgaucgc agauuuggcg ccguggcugg gaauguguac      360

gcccugcggg cuagagcccu ggacgagaag cucccucugc accaucuugg acgcgcgcug      420

uuccaccuca accagcggag aggcuuuaaa uccaaucgca agaccgauaa gcgggacaac      480

gaucaaggga aaauugccuc uggaaucagc agacugggcc agaaacuuga cgaggcuggg      540

gcccgcacgc ugggagaguu ccuccaucgg cagcacuccg cuggcaaaug ggucagagug      600

cgccccagcc ggguuccuuc uaaggaugac aaagaggaag acggcuaugg guuuuacccc      660

gagagauccu uccuggagcg cgaauuugcc gccauuugga aggcucaggc ggccuucuau      720

ccugagcugc ucaccgaaga acggggagau caucugcaca gagugauguu uuuccaacgg      780

ccgcuuaagc gccccaaagu cggcaagugc agcuuuuacc cugacgaaga uagacugccc      840

aaagcucacc cucucuucca ggaguuccgg cuggugaagg agcuuaauga acuggagguu      900

gugcauagag gggaccgccg ggaaaaacuc acacuggagc agagagauac ccuguuuaac      960

cagcuccgca cacaaagauc uuccgccuuc accgcccugc ggaagacgcu uaaagcuccc     1020

acaggaaccc gcuuuaauaa ggaaagcgac gccagagaua agcugaaagg cgacgagguc     1080

gcggcugaga ugucucacaa ggaucgguuc gggcaggccu ggaucggaau gccgcucgcc     1140

gaacgcuggg cugugauuga gaugcugcuu gacacagaag auccucugga ggugaaagcc     1200

uggcucgaag aacauuaugg cuuugcugcc gacagaaucg acgcgauugc cgcugccaag     1260

cugcccccug gcuacgggcg gcugggagag accgcucucc gcgauaugcu ggacgaacuu     1320

aaaaacggca ccgucaagga ugacuccggg agagauguug ugcugacaga gcggcaggcc     1380

auggacaaua ucuggaagga uaaccccgcg cuccagcgcg uggucgccga cgcuagagaa     1440

cugcccccuu accaagaggu gcugggacgg agaaucccgc ccggcacggg gaaaccugag     1500

gaucccuaug acguucagaa gggccgccuu accaauccua cagugcacau uggacucaac     1560

cagcugaaac gggucgugaa ugcccugguu agacgccacg gccggcccga ucaaaucgcu     1620

cucgaacugg ccagagagcu uaagcugaac gaggaacaga aagccgaaaa ggaccgcgag     1680

auugcucgga acaccaaggc ggcccaggcu agaagcgaga aacuccgcca gcuggauauc     1740

gacgacaaug gguacaaccg ggugcugcuu aagcucuggg aagaucugaa ucugagacaa     1800

ccggagaacc gggaauguau uuauugcgga aaagccaucg gcaucgggac acucuucucc     1860

aaucaggucg acauugauca uauccugccu uggucuaaga cccuugacga uagccaggcc     1920

aaccgcauug ugugucacgu cgagugcaau agacagaaag gaaaccaugc ucccgccgaa     1980

gugccucaau ggcacgaccg guacgaugac auccuggcgc gcgcugagag acucccccgg     2040

aauaaggccu ggagauuugg ccgcgaggcc augaagaaau ucaaggauga cgaauccuuu     2100

cuggcucggc agcugacgga uacacaguau cuuagcagaa uggcccauga guacguugcg     2160

gcucucuauc cugacgagga acccgaugaa uggggcgugu ucacccgccg gaaccacguc     2220

agagugauuc cggggcgcau gaccgagaug cugcggagac aguggggacu gaaugccauc     2280

aucucugacc acgaccaaaa gaaccgcaau gaucauagac accaugccau ugacgcuauc     2340

guugugggcg ucacaacccg gcgccuccug cagagaauug ccacagcugc cgggggacgg     2400

gaggcgcuug cccuggaaga gacgaucggc aagauugaac cucccuggcc cggguuuuac     2460

gaugacguga aagaggcuau cgaucgcauu gugguuuccc acaagccuga ccacggaagc     2520

cucccgaaac ccggcgagcc ugccagaacc gcugggcagc ugcauaacga uacagccuac     2580

ggcuucaccg gagaggaacg gaagggcguc cccaucaugg ugcacagagu gccuuuugcc     2640

acacugaccg aaaaggacau ccccucuauu cgcgaucggc agcuugcuga ggcgcucuau     2700

ggggccaucg cuggaauuag cggcaaagcc gaacuggccg cugcccuggc gagauucucc     2760

gcuucugggc cuuacaaggg aauccgccau guccgggugg uugagcccgu gagcgucauu     2820

gccaucaaag acagagaugg cacgccguau aagggguaca aaggcgacgc caauuaucgg     2880

uacgacgugu gggagcuccc uaacggaaag uggacccacg aaguuguguc cauguucgau     2940

gcucaucgcc ccgacuggca aagcgagauc agaaacaaga auccugccgc gcggaaaguc     3000

cugucucuuc accagaacga uaugguggcu uaugaacacc cccaggacgg cuacacaauu     3060

gggcgcguug ugaaguuugg auccggcggg caaauggccc uggccggaaa uagagagggc     3120

ggcgcucuca aaagccggga ugccgacccg gaagaucgcu ucagauacuu uuauaagucu     3180

gggggagcua ugcgggacgc ccgccugaga cagguccggg uggaugagac cggcagagug     3240

uucgacccug ggccccagga ucgcgagucc cggaacgcga gaaaacagag cgccgcugga     3300

uga                                                                   3303


<210>  3911
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3911
augaagaccc ugggccucga ccuggggaca aacagccuug gaugggccau cguggagacg       60

agagaugaac ccaccaaaua cuccgagggc ucuauugucg cuagcggggu ucggaucuuc      120

ucccagucug aaauggccgg acgcgacccu caaagcaagg cgucccuggc uguggccaga      180

cggcaggccc gcggcucuag acggcgcaga gaucgguauc ucaaacgcag acagcggcug      240

guggaccuuc uggucgaucu cgggcugaug ccggcugaca aggccgcucg cgccgagcug      300

gugaaaaauc acggagaugg caagacaggc gaccucagcu ccagcguuua cgcgcugaga      360

gcccgggcuc uugaugaagc ccugucuccc uaugagauug ggcgcgcuau cuuucaucuc      420

aaccagagac ggggauucaa guccaauaga aaagccgaca gcaacgaucc ugagcaaggc      480

gugauuucug aagggaugcg cgcgcuggac gagaagaugg ccgaagcugg agcccggacc      540

aucggcgagu ggcuucacau gagacgccug caggggcucc ccguccgggc uagaaucgcc      600

ccugauggag agggcuacgc cuuuuauccc ucccgcggcg cucuggaacg ggaguucgac      660

agaauuaugg cgcgccagac acuguuucau accgaccucc ugacagauac cgccauccgg      720

gaacuuagag aggugguuuu ccaccagcgc ccucugcggc cggugaaacc cgggaagugc      780

agcuacaauc cucucgaaga gagacuggcu aaagcccauc cccuuuuuca aaaguuccgg      840

cugcucaagg agcugaacga acuggagauu gucggagaag accagaaaca caucaagcuc      900

acgccugagc agcgcaaugc ccuggcucuc cagcugagaa ccucucucac aaaacaaggc      960

aaggugcccu uuuccaaacu gcggagcacc cuuaagcugg ggagagaagu gcgcuucaac     1020

aaagagacag auaaucgguc cgaccucgag ggagauguca uuuauuuuag aaugucucgc     1080

ccggaauguu ucggcaacag augggccgac cugccuguug agaugcaggc ggcuaucacc     1140

gaacggcugc gcacggagcc cgauuacaag acacucauga auuggcugaa ggccgaaacc     1200

gggcuugacg aggugcacgc cagagcugug gccgauacac cuguccccga cggauuuggc     1260

cggcugggcc agagcgcucu caccgcccug auugaugcgc uggagaacga agugggggag     1320

ggaggcuucg uuaucaccga agccgaggcu gccaaacgcg uguacgggag aacaaauucc     1380

gaggcugacc ccgaccggaa gggagucgau gaacuuccua aauaucaaga ggugcucgcc     1440

cgccauaucc cgcccggcac gggggaaccu gacgaucccu acgacgauuu caugggcaga     1500

auuaccaacc cuacaguuca caucgcgcug aaucagcugc ggcgcguggu caacgcccuc     1560

auuagaaccu auggaaagcc cgaccggauc gcuauugagg ugggcagaga ucugaaaaug     1620

aacgaccgcg aacgggauga ggucaauaga cgcaucgggc ggaacacaaa ggccgcuauc     1680

gagagaggag ugaaacuuac cgaaacguuu cgccagcugg acacaggcua caaucggcuc     1740

agacuggagc ugugggaaga ucuuaaccag aaaccggaga aucgcguuug cauuuauugu     1800

gggaaggcca ucgccggaca uaugcuuuuc aacggcgaaa ccgacauuga ucacauccug     1860

ccuuacagca aaacccugga cgacucuaag gcuaaucgga uucucugcug uacauccugc     1920

aacagagaga aaaagaaucg gcccccuagc cgcgugcccc aauggagauc ucgguauggg     1980

gagauccugg cgcgcgccac cacacuuccu aagaacaaac aguggagauu ugcugaagau     2040

gccaugacgc gguucggcga ggaagaggga uuucuggcca gacagcucac cgacaugcag     2100

uacaucagcc gccuggcucu gacauaccuu gcccaccucu augauuacga agaggucgac     2160

cuggauggca uuuauaagcg gcaugcgaga gugcgcgcuc ugcccgggcg gaugaccgag     2220

augcucagac gccaaugggg acugaaugaa cuucugcacg accauaaccu caucggcggg     2280

gauguugcca aaccgaagaa ucggcuggac cacagacauc acgccauuga ugcuaucgug     2340

auugccugua caucccgcgc gcugauccag agacuugcua ccgccucugc cgcugccgag     2400

gaacggggag cugagcgcgu cguggagaga auugacccuc ccuggagcgg cuuccgggaa     2460

gaugugcgcg ccgcgguugg ggccaucguc gugucccaca aacccgacca uggcacggug     2520

ggaagagagg gcuacgcuaa ggggcgggga cagaccgccg gcaagcucca caacgauaca     2580

gcuuaugggc auaccggaga aaaagacgcc agcggcaaug acaucgucgu gagacgcauu     2640

gccguuacag auauccgguc uccugcugac auuagaaaga uccgcaccaa cgcgcacggg     2700

cacuccgagc ugcgggauag acuguacgaa gccacgcggg gccucgaggg aaaggcuuuu     2760

gagcaggccg ugcuggacuu cgucaagggc gaugccaaau uucgcgggau uagacaugug     2820

cggaucacag aaguugcuaa cccggugugg aucacccacg gagacggccg ccauaagaaa     2880

ggguaucuuc ccggaggcaa ugauagauuc gacgucuggg agcuggccga uggccggugg     2940

gacgcggaag ugguuacaac cuuugaugcu caccgcccug gguucgugcc cagaaugcgg     3000

cucgagaacc auaaugcccg caagauuaug agccugaaga aaggagacau gaucgccuac     3060

aacgacccug ucucuggcca aagaauuauc gcuauugugc ggaaguuuga ucagagaaau     3120

aaacagcugu accuugaccc ccacaacgaa gccgggaauc uggaucgccg ggagaaggag     3180

aaaaccuaua agccucucag acccaugccg aacccucuga agaaauaccg ccccagacag     3240

gugcggaucg acgaaaucgg acaagucuuc gauccuggcc ccugguggga gcgccaggac     3300

ggguga                                                                3306


<210>  3912
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3912
auggagcaga ccagccugga ccucucccaa gccaugaaga aaguggcuca gccccagaag       60

ccuuacagac ugggcgucga uguuggggug gccucuaucg gagcggcugc cgugacagac      120

acggaaauuc uuggcaucca cguccggacc uucgagaaag gggaaauuaa cggaaagagc      180

ggccgcaaac cgcugaaugc cgauuauaga cggcagaagg ggcaacgcaa gagacuccgg      240

cgcaaaaaga acagacugcu uucccugcgg cgccucuuua gacggguggg aaugaucgag      300

ucugcugcca uugaggcuuu ccccaucagc uccacaucuc cuuggcagcu gcgcaccgaa      360

ggccuggaca gacggcucac aaaugaggaa ugggcccagg uucuguacca uaucgcgaaa      420

caccgcggcu uucagagcaa cagacgggug gauauggacg aggccaccaa acuuuccgau      480

gcugccgggg acagccccga aacaaccacg aauaccgagu cugauuccga cacaaagaag      540

augcuggcug gagucgccga uaacaagaga cuccuggcgg agaaaggcua ucgcaccauu      600

ggggaagccg uggcucggga cccuaaguuc agccauagaa uccgcaauaa agaggaaucu      660

uacucccaca caauuggacg ggaugagauc aacgccgaac uuaauugccu guuucaagcu      720

cagaaccauu auggcaaucc ccacgccacc gaggagcuca gcgaacagau uaucgaccug      780

auuaacugga gacgcaagac gguuacaggg gacgauauca aacggauggu gggaagaugu      840

acccuggagc cuaaggaaua ccgcgccgcu auccacucug ucuccgcgga ggaguuccgg      900

uggcucagca cacuguccag acuucgcauu gugacccagg gcgaaucucg gccgcugacc      960

aaaugcgagu gggacaaggu ggucgaucuc cccuggagac aagaagccau cacauauacg     1020

agcauucgga augcuaucgg ccugcgccau gacauuagau cccgguuuaa caucagauac     1080

gagaccgggg gaaagaaaac aaagaccgau accaaaaaug aaaccacgaa gggccagagc     1140

gccgacgccg uuacaucuga ggugaaaacc gcugaaaagg agaccgccuu caaggcgaaa     1200

ggguaccaca uccuucgcaa ggcuuauaaa gccuuuggau acgaugagca gugggaccgg     1260

gauagauuca acacagaacu gcucgacacc cuggccacag cugugacguu uuauaaggau     1320

gacuccgaga uucaagccca ucuggaaacc aacgacacac cugaguacgc uauccagauu     1380

gcccuccgcc ugcccacccu uacaaaauuc agcaagcugu cuaauaaggc gauccucaaa     1440

auucugccuc acaugagaga gcggcagagc uauuccaacg ccugucuucu ggcuggcuac     1500

aagaaucacc auaaacagua ugaugacaca ccccaaaacg ggaagauccc gcgcucugcc     1560

aucgaacaca uuaaaaauac ggucguguac agaagccuca accaggcucg gcgcguuauc     1620

aaugccaugg ugaaggagua cggagcgccu caggaagucc auauugagcu ggccagagau     1680

auggaaaagu ccgcuaaaaa gaaagacgag aucgaucugu ggaugaagaa acggaagaaa     1740

aagaacgacg agcuccgcga aagauuuggc aagaaucuga gccgggauga gcuucugaaa     1800

uauaagcucu acgaggaaca gaacggccaa ugcgccuauu cucugcagcc cauucagcug     1860

gggcgccuuc ucgaaccuaa uuacguggag guugaccacg cucugcccag aucccggagc     1920

auggauaacu cuuauaauaa caaagugcug guccucacca agcagaauag agacaaagga     1980

gaucgcacac ccuucgagua cuucggcgaa gaccaagagu ccguggauug gaagcgguuu     2040

accaacuggg ucgaaagcaa cccucagaug ccgcaggcca agagacgcaa acugcuuaca     2100

aagucccugc agaaagggau ccucgacaau uucucugccc ggaaccugag cgacaccaga     2160

uauauugcuc gcgagcugaa gacgcuuauc gaacauuaca uccucaauac acccgagcgg     2220

gagaaaacca aguacgaaag augugugguu auuuccggaa agcugacagc gcaaaugcgc     2280

cagcuguggg gcaucucuaa agcccgggau gugagcgaca gacaccacgg guccgaugcu     2340

gccgucauug ccugcaucac cccuagcaug gugcggcgca uuaccagaua ucuccagcug     2400

caagagcggc gcgcuaugcc cucugccccu ggcaagggag acgaacaucc cgcgguugug     2460

gcuagagcca ucccgcacgc cccugucccc uggccuacau uugagcauga ucuugaggcu     2520

gcccuggaaa aagugcucgu gucccggccc cagagcagaa aguggucugg cccuauccac     2580

gcggacacga uuuacgggga gcacaaagau caggaaacca acacaguugc ucccacccag     2640

cgcaucaagc ugacacaacu gaccaagucc aauuauaaaa acauugucgg auacgacgag     2700

gaacggaaua aggauuucau cgaccagauu gaggagagac uugaauguca uaacuuuaau     2760

ggcaaacagg ccuucgggac gagccagucu ccgaucuauc gcaucaagaa aaacggagag     2820

cucggcccug aagugaagaa agugacaauu aagcugccca ucaagaauag cgucuuucgg     2880

auuagacgcg gguacgugaa aaacgccaaa aauuccacca ucuauauuaa caucuaccaa     2940

aauaccaaga caggcaaguu cgagaugcac cccguuuacg cuaaagaucu gaccagcucu     3000

gccaacgcuu ccaagagcuu ugaauauucc ccugaccucc agcuggugga uacaauccgg     3060

agaggagacu acauugagau cacgcgcaga aacggcgagg ucauuucugg guauuacgcc     3120

acccauaaua ucagcacauc cgugcuugaa cugcagccgc acgacaccac agauucucgg     3180

aacaaucgca cccccgcgau uaagacgagc cucaacaucg agagauucca uauugacauc     3240

cugggacgga aacgcagagu ugugcggaga gccaagaaug gccuggcuga ugaccgcgau     3300

aaauga                                                                3306


<210>  3913
<211>  3309
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3913
augggcuaca gaaucgggcu ggacauugga aucaccagcg ugggcuaugc cauucucaag       60

acagaugaga aagggucccc cuacaagauc gaacaccuga cgucugucau uuucccuauc      120

gcugagaacc cgaaagacgg aaagagccuu gccaugcccc ggcgcgaaca gagaggcgcg      180

cggcgcagaa aucggcgcac caaauuuaga aagcaucgga caaagaugcu guucguucgc      240

uccgagcucc ugaccaucaa acaaauugaa gcuaucuauu cugagaagca gaaacuuagc      300

uccauuuacg agcugagaac aaaggcccuc aacgaacggc ugaccaauga ggaacuguuu      360

cgcauccucu auuucuuuuc ugggcacaga ggauucaaaa gcaaccggaa guccgagcug      420

acagagaagg auaugggccc ugugcuuacc gccauuaaag acaucgaaga gaagcuggcu      480

gauggcacgu accgcacccu cggggaauau auggagcugg acgaaaaaua ccaggagcau      540

aagagaaaua aagauggaau ugaccgguac cuuggcacag ccaagagaag ccugaucgag      600

gaugaaauca aacagauuau cauugcucaa cagcaguuug ggaacaccaa gcucacaucu      660

ggauuccagg uggccuuuau cggcgacgag gaacaaaccg ggauuuucaa ugaucagcgc      720

gaguuugaag acggacccgg caagcagucc ccuuaugcgg gcaaccagau cgagaaaaug      780

aucggggccu gcacguucga ggaaaaugag aagcgggcuc ccaaagccag cuacacauuu      840

caauauuucg auuuccugug uaagcugaac aaucucaaau acagagaaac cauuggaggc      900

gaguaucgcu uucugucucu ugaacagaag aacguccuga aggagaaagu gcucacaacc      960

cagaagauca ccuucuccgg gguuaaaaag cugcuugacc ugccugagca cgcucaauuu     1020

aaucucguga acuacggaac aaaaagcacg gucaaggaaa ccgagacaaa gucuguguuc     1080

uaugccauga uuuccuacca uaaacugaag aaagcgaucc cggcccccau uuuuagcaau     1140

cuguccaccg cugaacucga cgagaucggc cggauccuga cacuuuacuc uagcgauaag     1200

gggcgcaaaa acgaguucua uuccagacug gccauuccug aagagguugu ggaagcucuc     1260

cugcccauca acuuuagcaa guucggaaau cuuucucuga aggccaugcg gaaaauuauc     1320

ccuuaccucg agaugggccu ggucuauucc gaagccgcua cccaggcggg cuacgacuuu     1380

agacacaaga aaauugauca ggccuauauc cacgagaacg ugacgaaucc cguggucaag     1440

cgcgcugcca gccagacaau uaaaaucguu aacaauauca uucggaagua cggggagccg     1500

gacggaauca acauugaacu ggccagagag cucucuaaaa auuucagcga acgcaagaag     1560

accgugaaau aucaagagga aaaccgggcu agaaaugagc ggaucgccga uaagcugcgc     1620

gagugcgaac uuccugugaa cggcgcgaau auuaccagac ugaaacucuu ugaggaacag     1680

ggggaguucg accccuacac aggacugaag aucccuuuug aucgggcuuu cuccgaagag     1740

uacgaggucg accauaucau ucccuauucu aaaacccugg augacagcua cggcaacaag     1800

auccuugugu ccgccaaggc caaucaggaa aaagggaacc gcauucccau ggaguaucuc     1860

cagggcaagc cugaaagaau caaaaagcug gagagcauua cagcuacgau caaaaauccg     1920

aagaagcggg agaaacugcu caagcaacag cugaccaaag aagauguuga cggauggaag     1980

acaagaaacc uuaacgacac ccgcuacauc ucuucccugc uccacgauua uuucaaucag     2040

aacauuagcu uugccaaacu ggagucugaa aagaagaaaa gagugaucgc ugucaauggc     2100

gccauuacau ccaagcugcg ggcgcgcugg ggguucaaca aaaucagaga gaauggagac     2160

gaacaucacg cccuugaugc ucucauugug gccuguguua ccgacaagua caucaaagag     2220

aucacgagcu uuuccaagcg gaaagaggug cgcuucaacg aaaaucugug gaagagagag     2280

aagacacccc aggaaauuga gcaagaaguc gugcacuuua aaaaggauua ugaccggauc     2340

uucaaccagg cuuuuccucu gcccuggacc aauuuccgcg aggaggucuc uugcagaaug     2400

agcgaaaacc cugccgagcu caugaaaaau cauaccuggg aaacauacac cgaugacgag     2460

auugcggccc ugcggcccau cuuuauugug cgcaugccga acaugaagac guccggcccu     2520

gcucaccagg aaacaaucag aucugggaaa cuugaggaga agggacugaa uauuagccgg     2580

guuuccaucg aaaagcucaa acuggauaag gagggcacca ucaaaagcgg gaacgcccag     2640

uucuacaagg acucuggcaa uggauggcaa cugcuucaug auaaacucaa gguggcucug     2700

gaaaagcacg acggcgaugg guccaaagcc uuucccgacg gagucuucaa guaucaguac     2760

cuggacaaag agcauacagu gagaaagguu cgccucgugg ccaaaagcac ccugcagacc     2820

ucucuuaacg agaagaaguc cgucgcugau aacggcagca ugauucggau cgacguguuu     2880

agaaaugaac aagggaaaua ucaguucgug ccuauuuacg cgaaggaucg cguugccaaa     2940

aagcugccca accaggcugu cgccguggga aaaaucgcca aggaguggcc ucucauugaa     3000

gagucugacu ucaaguuuag ccuguauucc aaugaucuga uccgggugaa acacaagaaa     3060

ggcgucaagc acgugagcaa agacgggaac aaggaagaga aaucuaagac agauuucuuu     3120

ggcuacuaua cgggagcuga caucgccacc gcguccauua gcggcaucuc ccccaauaac     3180

uucuuugagu ucagaucuau ggggcuugcu acacucauua gcaucgaaaa guacaaaguu     3240

gauuacuuug gaaauuauca ucaggugaac gagaaggucc gccaaaaauu cccgaagaau     3300

cggauguga                                                             3309


<210>  3914
<211>  3312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3914
augaaguacg acaucggcau ugauaucggg guggccagcg ucggagaggc uguuauugac       60

caggaaggca acauccugga ggccugcucc aaucucuucg augaagcgga cgcugccucu      120

aacguggaua gacggaauuu ucgcgagggg agacggaaca aacgcagaga acggacccgc      180

gugaaugacu ucaagaaacu guggacaaag uuuggauucg agauucccaa aaacgucaug      240

aaugauacga uccuucugag aaacaagggc aucaagugug agcucgaccu gaccgaacuu      300

uauagcgugc ugcucuacau gcugaaacac cgggggauuu ccuaucugga ggaugccauc      360

gacgaagcua agggaucuaa uuacgccaaa ggcauugcuc ucaaccaaaa ggagcugaaa      420

gagaagcucc cuugcgaaau ccagcuggag cgccucaaga uuuauggcag cuacagaggg      480

gauuguaucg uuaaaaagga agacgaggau gaauaucauu ccaauguguu uacaauuucu      540

gccuacaaaa aggagcugga gauccuuuuc cggaaccaga aacugccgga agaguuuauc      600

cagggauaca ugaagauuuu cgaacgcaaa agagaguauu acaucggccc cgggaaugaa      660

aagagccgga ccgacuaugg agucuacaca accaacaagg acgaggaggg ccacuauauu      720

acagauaaaa auaucuuuga aaagcucauu gggaaaugcu ccguguacaa ggacgaguuc      780

cgcgcggccg gagcuagcua uaccgcccaa gaauuuaacg uucugaacga ucugaauaac      840

cucacgguga auaacagaaa acugaccgag aaugaaaaga agaaaaucgu cgagaagauc      900

aaaucuucca acgcugugaa uaugcggagc auuaucaaga aagugcuugg cgaggacauu      960

gaacaguucg gcggggccag aaaggauaag gcggacaaag agaucuacca uacauuugaa     1020

gucuaccgca agaugaaaaa ggaguucgag aaaaacggaa uugauaucgc cgacuucucu     1080

cgggaaaagc uggaugagau cggcaauauu cucacccuga acacagaaac cgaggggauc     1140

aagggagccc uucacgaagc uaaaaauucc uuuacguaua gcgagauuga gacacugauc     1200

gacauuagaa agaaaaacuc uucccucuuc agcaaguggc agucccuguc ucugaaaauc     1260

augaaugaac ucauuccuca gauguacgcu caacccaagg agcagaugca gcugcuuacc     1320

gaaaugggcg uuuuuaaauu caagagcgug aaguuuaaaa acuccaagua uaucccugug     1380

gaggaaauca uuaaagauau cuacaauccc gucgugguuc gcagcgugcg gacaaccauu     1440

aagaugauca acgagaccau uagaaaauau ggguacccug acaacaucau cauugagaug     1500

ccgcgcgaca aaaauucuac agaagagaag gaacggauca agaaggagca guccaaaaac     1560

gaaaaggagu augagggcau ucugaaaaga aucaaggaug aauacgacau ugagaucaau     1620

cgcgaugacu accggaagca agaaaaacuc gcccugaaac uuaagcugug gaacgagcag     1680

gagggaagau gucccuauag cggcaagaug aucucuauuu acgggcuccu ggaagauccu     1740

cagcuguucg agaucgacca uauuaucccc aaaagcauuu ccuuugauga cucucgcaau     1800

aacaaggucc ucguguaucg gagcgaaaau caaaagaaag gaaacaauac gccuuacaug     1860

uaucugucca agggcgauaa aaacuggaau uacgaccagu auaagagcua cguucuuggg     1920

cugaaaaaca agggaaauau cucugauaaa aaggugaaca auaugcuuuu cauggaggac     1980

aucaacaaga uugauguccu gaaaggcuuu aucaccagaa accugaauga cacacgguac     2040

gccucccgcg ugguccuuaa caugcuccag agcuucuuua augaaaagga cgggugcccc     2100

accaaaguga agguuauuag aggcucuuuc acacaccaga ugcgggugaa ccugagaauc     2160

aauaaagauc gcgacgaguc cuauagccac caugcugucg augcgaugcu gauuguguac     2220

ucccaacggg gauaugaggc cuacagaaag auccaggagg cuuguguuga cuuugaaacc     2280

ggcgaaauua ucgaugagga acgcuggcag cagugcaucu gggggaagga cuaugaucaa     2340

gugauguacg aggccaaaug gucuaagauu agagagaaaa ucauugaagc cgagaagaac     2400

gucaaauauc accaucgggu ggacaagaag gugaaucgcg gacucuguaa ccagacgauc     2460

uacggcacaa gaauuaaaga ugggaagauc uacaaaauca gcuccuauaa uauuuacgac     2520

aacaaggaau gcgcuucucu gcuuaaaaug aucaagggag gcaaggagag caaaauucug     2580

auguauaauu acgauuccaa gaccuaucgg gacaugauca aaauuaucga ugaauacagc     2640

gacgagaaga acccguucgu ugccuauaug aaagagaccg gggacuacuu ccgcaaguac     2700

gcgaagaaac acaauggccc uaagaucgaa aaagucaagu auuacaaaga ggaagcugga     2760

ucuuguauug auaucuccca caaguauggc uacgagaaag ggagcaagaa ggugauucuc     2820

ggaucucuga accccuauag aacagacgug uuuuucaaug agcggcugaa aaaguacuau     2880

cuuaucggca uuaaauacaa ccauaucaag ugcgaaggga acaauuacgu cauugaugag     2940

gacgccuaua acggacuccu gcgcgaugcc ggcgugaucu cccacgaaaa uaccaucaac     3000

gaccuggaga aaguuggggu gaaguaccuc uuuagccugu auaagaauga uauuaucaaa     3060

uacgaaaaga acggcgagau guucgucgag agauuucuuu cucggacaau ggaauaugcu     3120

aauaacuaca uugagacgaa accuauccau aagcccuucu uucagaaaaa ggacaagaaa     3180

ggaaauauua agaacagcca gaaucuggug ggccucucca aaaccaagua uaucgggaaa     3240

cugguuacag augcccuggg aaacaccuac augguggaaa aggagaaguu cagccuuauc     3300

gucgacauuu ga                                                         3312


<210>  3915
<211>  3315
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3915
augaagcuga uccucgcccu ggacauuggc aucgcuagcg uggggugggc cguccuugau       60

aaaaaguccg agaccguucu ggaagcggga ucuaacauuu uccccgaggc uagcgccgcc      120

ggcaaucagg ugagacggga aaugcgccaa gcuagacgga ucaaacgcag acagcggaca      180

cgccucgacg auuuuaacaa gcuguggaga aagaauaacc uuuccauucc ucaguacaag      240

ucuacggaga ucgugaaucu gaaggucaaa gcccuccggg aagagaucag ccuggacgag      300

cuguaucuca uucuguacuc cuaucuuaag caccgcggga ucucuuaccu ggaagaugcu      360

caggacgaua gccucuccag cucuaacgcc uauaccaaug gacugagaga gaacgcgaaa      420

gcccuugaaa agcauuaccc gugcgagauu caacgggacc gccugcagac aaucggcaaa      480

uauagagggc agacccagau uacagaugac aauaacgaga agaucgaucu cuccaaugug      540

uucaccauug gagcuuaccg gaaggaaauc aaaaagguuc uggagacaca aaaacuguac      600

cacaacgaac ucaccgacga gcugugugau gacuauaucc uuauuuuuaa ucgcaagaga      660

aaauacuaug aaggccccgg caacgaaaag agccggacgg acuacgggag auucaccaca      720

cgccuggauc agaauggaaa cuauaucgac gaggaaaaca uuuuugagaa acucaucggc      780

aagugcucug uguacccuga ugaacugaga ggggccgcug ccuccuauac cgcgcaggag      840

uucaaucuuc ugaacgaccu caauaaccug acaauuaaug gaagaaagcu ggaagagagc      900

gagaaacaug ccaucgucca gcgcaucaag accucuaaca cggugaauau gcggaaaauu      960

aucgcugauu guaugggcga aacaauugag aacuuuuccg gggccagaau cgacaagagc     1020

ggaaaagaac aauuccacaa guuugagacc uacaauaaga ugcgcaaauc ccucucugaa     1080

auuggcacag auaucgacga guucagccgg gaugagcugg acaagaucgg cuacauuaug     1140

accaucaaca ccgauaaaga agcucuuaug gaggccuuua augaauccgg gcugaaccuc     1200

gacgacagcg uuaaggagug ccugauuucu uucagaaaag ccaauuccag cucuuuuagc     1260

aaguggcagu ccuucucucu uaagaucaug aacgagcuga uucccgaaau guauauccag     1320

ccuaaagagc aaaugacacu ccugacggaa augggagugg cuaagggcag cuccggggau     1380

uucgcgggac ugaauuacau ucccgucgac gugaucagcg augagaucua uaacccugug     1440

guccgccggu cuguuagaau uuccuuuaaa auccucaacg ccgugcugaa gaaauacaag     1500

gugcuugaca ccauugucau cgaaaugccg cgcgaucgga auagcgacga ggagagaaag     1560

cggauuacag auucucagaa acugaacgaa aaggagaugg aguucaucga gggcaaacuc     1620

gcucagaucu auaauauuaa gcuguccagc uccgacuuuu cuagccagaa acaacugucc     1680

cuuaagcuca aacuguggaa cgaacaggau gggaucuguc uguacucugg acgcaccauu     1740

gaccccgagg auaucauuaa gaauccugac cucuucgaga ucgaucauau cauucccaga     1800

agcaucuccu uugacgacag ccggucuaac aaggugcugg uuuauagauc cgaaaaucag     1860

aaaaagggca accagacacc uuacuucuac auuacccaca gcgauggcgc cuggacguau     1920

gagcaauuua aagccaaugu gaugaaccuu ucuaagaaaa aggaauacgc uaucucccgc     1980

aagaaagucc agaaucugcu cuauagcgag gacauuacaa agcgggaugu gcugaaaggg     2040

uucaucaaca gaaauaucaa cgacaccgga uacgccucuc gcguugugcu gaauaccguc     2100

cagaacuuuu ucaaggcgaa agaagcugau acaaagguga aggucauuaa aggcagcuau     2160

acccaccaga ugagauccaa caugaaacuu gacaaaaauc gggaugagag cuacucucau     2220

cacgccgugg acgccaugcu caugggguuu uccgagaugg gauaugaagc cuaccgcaga     2280

cugcaaggcg aguucaucga uuuugaaaca ggggagauuc ugaagaacga cauguggaau     2340

gagaaaauga gcgaugacgu uuacguggau uaucucuacg gccggaagug guccgcuauc     2400

cgcaacgaaa uugucaaggc ugagaaagac gugaaguucu ggcauuucau cguuagaaaa     2460

ucuaaucagg gacugugcaa ccagacgauu cggggcaccc gcgaauauga cgggaagaaa     2520

uacaagauca auaaauuuga uaucagaaca aaggagggag ugaaacuuuu caagaagcug     2580

gccuuuagcg aaaaagaguc cgaccgggag aagcuccugg ucuaucugaa cgaucgccag     2640

accuucaaua accuugugaa aauuuuugaa gacuacaagg augcgcccaa uccguucgug     2700

caauaugaga aggaaacagg cgacguuguc agaaaauacu cuaagcggca caacgggccu     2760

agaaucgaua aacucaagua caaagacgga gaggugggcg ccuguauuga uaucagccac     2820

aaguaugggc uggaaaagga cucccgcaaa gugauucugg agucucucgu ccccuaccgg     2880

auggaugugu auuacaagga gagcgacaaa auguauuacc ugguuggcgu gaagcagucc     2940

gacaucaaaa gccagaaugg agcuaacguc aucgaugaag accuuuauac ccggauucug     3000

aucaaugaga agaugauuga accuggccaa ucucgcgaug accucgagca gcugggguac     3060

gaauuucggu ucuccuuuua caagaacgau aucauugagu augagaaaga aggaaaggug     3120

uucguugaga gauuugugag ccgcacgaug cccaaagucc ggaacuacau cgaaaccaag     3180

cccaucaaua aagccaacuu cgagaagcag aauccugugg gccuggggaa gacaaaaucu     3240

auuaagaaau auagaaccga cauccuugga aaccgcuacc ucugcgagaa ggaaaaauuu     3300

acaaaguucu guuga                                                      3315


<210>  3916
<211>  3315
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3916
augaaccacu acgugcuggg ccucgacauc gggauuacca gcgucggaua uggcguuauc       60

gaucuggaca auuaccaguu cguggauuau ggggugagac uuuuuaagga gggaacagcc      120

gaagacaacg ucaaucggcg cacgaaaaga ggcucccggc gccugaagag acggcgccaa      180

accagacucg augacaugaa agagcugcuu cagaaggaaa agauuauguc ugagacauac      240

cauagcuccu augaucccua cgacaugcgg aucaaggggc ugucucagaa gcucagcaac      300

gaugaacuga ccugcgcuau ucugcacauc acaaaauguc gcggaaccac acucgaggcc      360

cuggacuccg cggaugagga cucugaaggc accaagagca uccuuuccaa aaaugaucag      420

gaccugaaga aauaucauua cgugugccuc guucaaaagg agagacugaa gcagagcggc      480

aaaauucggg ggacggaaaa caauuucaag accgacgauu acaucaaaga gcuugacgag      540

auucugucuc accaggaucu cuccgaagac cugugugaga agauccgcac aauugugagc      600

agacggcgcc aguauuacga aggaccuggc ucugagaaau ccccgacccc cuaugggaga      660

uggaucgcuc aaggagucgc cccuauugau cugaucgaaa agaugcgggg caaaugcagc      720

guguuucccg accagcucag agccgcuaag aacucuuaca cagccgagcu guucaaucuu      780

cugaacgauc ucaauaaccu ggagugugaa ggggagaaac uuaccacguc ccagaaacag      840

gacguuaucg auuuugugaa ugaaaaggga ggcauuacac cuaaacaacu ggcuaagcuc      900

cugaacgucg accugaauca gaucaaaggc uuccgcauug auaagaacga caagccccuc      960

cugaccgagu uuaaagggua uaaggccuuc aaaaagaucu uugacacaaa caauugcgau     1020

cuuuacaaaa aggaccugag cauucucgau ggacuggcgg aaaucgugac cggcaagaaa     1080

gggaucgagg agcggaagca ggccauucaa caggcuuauc cugaaaucga cgaggccauu     1140

guggaacagu gugcugucau gaaaggaaug acccaguacc auucccuuuc ucugaaggcc     1200

augcaccuca ucaaccaaga gaugcugcag agcccgagaa aucagaugca gguucugcac     1260

gaaucccaug uguucgauaa aaaccgcgug agccacaagg gccaaaagua cauugaggcg     1320

gacgaugagg ccauccucuc ucccgucgug aaacgggcuc agagagaaac auuuaagguu     1380

gugaaugccc ugcgcgagaa auauggggag uucgaaucca ucgucgugga gacgacccgg     1440

gacagaaaca gccaggaaca gaagaaacgc cuuaaugauu cucaaaagua cuaugagcag     1500

gaaaaccgga aaguugacga gcugcucagc caugaaggau acgauccuuc caagauuaau     1560

ggcaagacaa aacugaaggu gagacuguau cuucagcagg agggcaaaac cgcuuacaca     1620

aacgccccca ucgaccucaa ucugcugauu aaggaugcca ccgcuuauga ggucgaccac     1680

aucauuccuc ucucuaucag ccuggacgau uccauuaaca auaaagcgcu uaucaaccgc     1740

acggaaaauc aaaacaagag caacaugaca cccaucuacg ccauucugcg ggggaagcuc     1800

aauaucucuc uggacaccua caaaaccgug gucaagaaca auagaaacua uacaggcaaa     1860

aagcgguccu accugcuuua ugauaaagac auuaccaagu uuagcaauau gcaggaguuc     1920

aucgcucgca accucgugga uacaucuuac gccugcagag uugugauguc cacgcugagc     1980

gacuauuuca aggauaauga aauuuccacc cacguccaua cagugcgggg aagaguuacc     2040

aacauguuuc gcaaucggau caacaugcag aaagagagag aaaaggacua ccugcaccau     2100

gccguggaug cucucaucgu cgccucuauu aaaaagcuga aucuucugaa cacauaucuc     2160

cugaaauaca auuucaacga ccuguacaau gaggagaccg gggaaguguu ugcgguggga     2220

gaugaccaac agguucuuga uccgaaguau auccgcuuca uuacgcagcu caagaccauc     2280

uacgacgaga gcaacaugua uuacaacggc cugauugaaa gaaaugucau gcaguuuccu     2340

cccaucaaaa ucucccacaa gauugacaca aaaccuaacc ggcaaaucuc ugaugagacc     2400

auuuauagca cacgcaccgu ggggaaugaa gaguacgugg ucaagaaaua uuccgacauc     2460

uacgauccca aguucacgca gcugacagac gauauuauca acgacagacc ccucgaucgg     2520

uggcugaugu accgcaauga caaccagacc uuuaaaauca uucagagcau cauucuugau     2580

cauuuccaca ucuuuaagga cgaugagaag cacuauucuu guaccaaaaa gaaaggccaa     2640

auucaguacg cucugaaggg caaaaauccu cucuaucugu acaagcagga acauggaaag     2700

gugagaaaau augccaagaa agggaacgga ccggagauca caaugaugaa guacuucgac     2760

ggcaaacugg ggaauaacau ugacaucucc aaucacuauc auguucacaa caagaaggug     2820

guccuucucc agaucagccc cuaccggacc gauuuuuacg ugucuccuga aggaaaauau     2880

aagaugcuga caguucgcua cgccaaugug uucuauaaag aguccaagaa aaaguacguc     2940

auugacgaga agugguaugc uagcgagaaa aagcggaaaa acaucacgga ugacgauauu     3000

uuugugugcu cucugcacca ugacgaacuc aucggcauuc aaaagaaaga uggggccaag     3060

uacguuuaug accugagcac cgaagauggc ggaaagccca gacuuuacca cggcgagaug     3120

gaaauccuga aauucacagc uaccaauaac gacuccaaga acaucauuga ggugaaaccu     3180

aucuacacau auuguaagaa acagcucaug auuagcaucg ggcccauuac caagcuggcc     3240

aaauacgcga cggauguccu gggaaaugug uaugagguga agaagaacau caucgaccau     3300

cuuucucacc uguga                                                      3315


<210>  3917
<211>  3318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3917
augggcauca gaauugggcu ggacaucgga auugccagcg ugggcugggc ugucguugau       60

gacgauuaca acaucauuga guccggggcc aauaucuuca acgaagcgaa ggcugccucu      120

aauguggacc ggcgcaccuu uagacagaca aaacggcucc ugcgcagaca aaagacgcgg      180

cuucgcgauu ucgacauccu guggauuaac gccggacucg auauccccaa agagaaggug      240

aauaaacagc ugcaguuuag aguccagggc augaccaugg aacuuagcga gaacgaacug      300

uaugcugccc ucaagaauau gcugaagcac cgggggauuu ccuaccugga ggacgcucuc      360

gaugaggaac aaucuggaaa aagcacauau gagaagggcc uccaggugaa ccagaaagcc      420

cuuaagugcg acaaaaugcc uugugaaauc caguacgagc gccugaugaa guauggcucc      480

uacagagggc auauugaggu uaaggaugaa ucuggagagg aaaugauccu cagcaauauu      540

uucacccugg acgcguauug caaagaggcc aagaaaaucc uugaauguca aaagcaguac      600

cacuccuuua ucacagaggc uuucgaggau gacuaccugg cccuccugcg gcgcaaaaga      660

aaguauuacg aaggcccggg gaacgagcag agccggaccg acuauggaaa auacacaacc      720

aagcugaauc ccgaaacggg cgaguaugug accgaagaga acauuuuuga gaagcucauc      780

gggaaaugcu cugucuaccc uaaggaaaug cgcgcuuccg ccgcgagcua uacagcccag      840

auuuacaaua ugcugaacga ucuuaauaac cugaccauca acggaagaaa acucgaguac      900

gaagagaaga aaucuauugu ggaagagguu caaaaggcuu ccagcgugaa uaugcugaag      960

aucaucaaaa aggucauuaa cgaggacauc acaaccauug aaggcgcccg gaaagauaag     1020

aaugacaaag agaucuucca uaagauggaa acguauaaca agaugagaca cuacuuugag     1080

aaaaauggcc uugaguucaa gaaaauuucu acagaugacc uggaugaaau cgggaacauc     1140

cucacccuga auacagagaa ggaagcuauu cuggaggccu uuaacaaauc ccagcucgaa     1200

cugagccagg aggagaagga agccuucauc gagauucgca agaaaaaugg aucccuuuuu     1260

aacaaguggc auucuuucag ccugaaaauc augaaggaac ucauucccga gcuguaucuu     1320

caauccaaaa aucagaugca gcugcucacc gacaugggcg uguucaagaa aaagaccgaa     1380

aucuuucaga acuacgauaa gauuccuaca gacgugauca ucgaggauau uuauaauccc     1440

gucguuaaac ggagcaucag aauuacggug aagaucguga acgcucugau uaaaaaguac     1500

ggguauccuc gcgaggucgu gaucgaaaug ccgcgggaca gaaauucuga cgaggagaaa     1560

aagcgcaucc aagaugaaca gaagaaaaac gacaccgaac ugaagaaaau uaucgagaag     1620

guugaguccg aauacggaau uaaaaucaca gauacccacu uccggaacca caagaaucuc     1680

agaaugaagc ugcgccuuug gcaugagcag gacggccggu gucuguacag cgggaaagug     1740

auugauaucu cugaacuccu gaacgacugc agcaaguuug agaucgauca cgucauuccc     1800

aaauccaucu cuuucgacga uagcagaucc aauaaggugc ugguuuauag cacagagaac     1860

cagaaaaaga ccaaucaaac gccuuaccag uaucuugaag gaaacggcaa ggaguggucu     1920

uuugacgaau acaugaauca ggugauugag cucaaagauc gcaagcugau cuccaaagcg     1980

aagaugaaca aacugcuguu ccgggaagac auuacaaaga ucgagguccu gaaaggcuuu     2040

aucgccagaa auauuaacga uacccgguau gcuagccgcg ugguccuuaa uucuuuccag     2100

aaguacuuug aguccaagca aagcgacacc aaagugaagg uuaucagagg guccuucaca     2160

caucagaugc ggaaaaagcu gcagcucucu aaaaacagag acgaaagcua ugcccaccac     2220

gccguggaug cuaugcugau uuguuacucc cagaugggau acgaggccua ucgccaacug     2280

caggaagacc uuaucgaucc cgacucugag aagauucggg aucucggccu guggaaggaa     2340

accaucacag aggcgaaaua cgacgagguc cuguauacgg cuaaguggcu cgaaaauaga     2400

gggaaaauuc aggaggccga aaagaaagug aaguuuuggc auaagguuga uagcaaagcc     2460

aaccgcgcuc ugugcaauca aaccaucaga ggaacacggg ugaucgacgg cuccauucag     2520

aagaucaaca aaauuaacau cuacagcguc gauggguaua agaccuucaa aaauaugauu     2580

cagaagggca aggagaaaga cuuucuuaug uaccgcaacg auagacgguc uugggacgau     2640

cugaugaagg uguaucgcga auacuccgac gccaaaaaug cuuucgccgc guaccaggag     2700

gagacaggag acuaugugag aaaguacgcc aaaaagcaca acggcagcaa gaucaccaau     2760

cucaaauauu gugcuaagga acuggggucu ugcaucgaua uuucccauaa auacggacug     2820

gagaaggaca gccggaaagu uauccuugaa ucucucagcc cuuaucgcuc cgaugucuac     2880

uauaacgccg gccugaagca auacuacauu guggggauca aaaauagcga ccuguguuuc     2940

cucaagggag aguauuuuau ugauucuaag agauaccugc agauccuucg ggaggaaggc     3000

gugcugacgg agcagcacac ccucaacgac cuggcugcca ucgggacaga guuccaguuu     3060

ucccuguaug aaaaugauua ccuugaauau gagaaaaacg gcgagauuaa gaccgaacgc     3120

uuccucagca gaacaaaacc caagcagaaa aauuacaucg agaccaaguc cauugacgcg     3180

ccgaagauca aaaacaagaa acaaaaucug gucggacugg ccaagucuaa aagcauuaag     3240

aagauccggg uggauauucu cggcaacaga uauuacgcuu ccaaagaaaa guuuucucug     3300

augguugaca aauucuga                                                   3318


<210>  3918
<211>  3318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3918
auggacaugg ccuggagacu gggccucgau cuggggacca acagccuugg augggcugcc       60

cugucccucg acgcggcugg cgcccccgau gccauccugg cugccggguc ucggauuuuc      120

ggagacggcc gcgauccuca gagcgggaca ucccuugcug uggacagacg ggccgcgcgc      180

gccgcuagac ggcgcagaga ucgguuuaag caacgccaga gagcccugcu caaacaccug      240

gaggcugacg gacuguuccc ggccgauccc gaaguccagc aggcgcucgc cgcucuggac      300

ccuuacgccc uucgggcucg cgcccuggau gaggcccucu cucugcauga aaucggcaga      360

gcucuuuuuc accugaauca acggcgcggc uuccagagca acagaaaggc ggaccggggg      420

aaagaugagg acgccggaaa gauugcuauc ggcguugaca gacucaaaga ugccauugcc      480

gcugccgggg cgcgcacguu uggagaguuc cugcggcaga gacgcgcugg cgccaccggg      540

gagaaucaga uccccuccgu gcggacaaga cugcgcgccg agaccggaga aggcgcuaag      600

ggcucugggu augacuuuua cccuagccgg gcccuccuga aggaugaguu cgacgcuauc      660

uggcaugccc aagcggaaca ccaucccaaa gugcuuacag augaggccua ucacagacug      720

cacgagauug ucuuucgcca gcggccucuc agagcuccga aggugggagc cugcacccug      780

guucccggcg aagcucgccu uccuaaagcc cauccccugu uccagcggag acggcuccug      840

gaggaacuga acgcgcucau gaucgugcgc gccggggcug ucgccgagag acugacaccu      900

gaacagcggg accuucugcu ccugaaacuu aaggauaagg gaaaggugac cuuuaaaucc      960

cugagagaga agguucucaa acuggacggc gaugcucgcu ucaauaagga gagcgaacac     1020

cggacggagc ugaaagggga cgaaguggcc gccgagaugg gaggcaagac cagauuuggc     1080

ucucucuggc cccaucuguc cacagaacaa caguggaccg ucauugagcg ccagcaagcu     1140

cuugagagcg augcggacga agccgcuuuc agaacauggc ugguggagac ccacaaacuc     1200

acggccgaac aggcccgggc uguggccggg gcgcgccugc cggcuggaca cggcagauuc     1260

gggcugacag ccaccgccgc ccuucucgcu gcgcugcggg acggacgcac accugagggc     1320

agagggccca acgcuccugc caccgaagcc ggccgggucg uuguguacuc ugaggcugcc     1380

gcuaucgccg gauaucauca cuccgaucau cgcagcggcg agguguuuac cgacgcgaag     1440

gggagacccg cccugcccua cuauggaguc ccucucgauc ggcacauugu gccgggcaca     1500

gcugaccccg augaaccuga cgaggccgcu cgcaucggga gacugacgaa ucccaccguu     1560

cauauugccc uuaaccagcu gcggagagug gucaaucgcc ucauccgggu guacggaccu     1620

cccgccgaag uugcucugga gcuggcgaga gagcuuaagc ucucugaaga ugagaaaaag     1680

gaacgcaacc gggaaaauuc cagaaaccgc cuggacgccg aaaaacggag cuccaagcug     1740

gcugagcucg gccagagaga uaaugggggc aaccgcgccc ugcuuaaacu gugggaggaa     1800

cucaauccgg agaacauccu ggaccggaga uguauuuacu cuggacggca aaucagcauu     1860

ggcaugaucu ucuccggggc cgcugaagug gaucacauuc ugccuuuuga cgccacacuu     1920

gaugacagca acgcgaauaa gauccucugc cugcgcgagg cuaacagaga caagcggaaa     1980

cgcucucccu ucgaagccug gggaggcacc ccucaguggg aggagaucgc cgaaagagcu     2040

ucccggcugc ccagaaauaa gcgcuggcgg uuugagccug augccaugga aagauucgcg     2100

gaggaagggg gauuucucgc ucgccaccug gucgacacac aguaucuugg ccggauugcc     2160

caugauuacc ugagaugucu cuaucccgac aaaggggaug gcagcucuca cguguggguc     2220

agcccgggac gccugaccga gauggugcgg agaaagcugg gccuuaacgg gcuccuggga     2280

gaccauaauc ugggcgccga gcagccuaaa aaccgcaagg aucacagaca ccaugcuauc     2340

gacgccguug ugacggcuau ucucgaucgg uccaugcugc aacgcaucca gagagccucu     2400

ggggaaggag cgagcgacgc cgagcuucug cggauuaucg uccccgaacc cuggacaggc     2460

uuccgcgaug accuccagag agcuguggau cggaucguug uggcccacag agcugaccau     2520

gggaccgucg ccaaggccgc uacccgcggc cgggaccaga cagcggccag acugcacaau     2580

gauaccgcuu acggauuuac aggcgaggcc gacgccaaag ggacgccuau uguggugcau     2640

cgcgcuccgc ugggagcccu uaagaaaccc gagcacaucg aucaaguucg ggacccucug     2700

cucagagcgg cucugcacga uuucaccgcc ggccucuccg ggaagcccuu ugaagaccgg     2760

auugccgcuu ucccucgccu gggaccccuu gauuauagag gcauccggcg cgucagagug     2820

guggagccuc ugcaggucau ucccauccgg gacgccgcgg gccgcgccua caaaggguau     2880

aagggagaua gcaacuacag auacgacgug ugggaacucc cggauggcaa gugggagcag     2940

cggguucugc agauguauua cgcucaucaa ucuggggaac cuccccgccc ucaccccgcc     3000

gcuagaaaag ugcugucccu ucaucgggac gacguccugg ccauugagag aggagagggc     3060

gggcgcgaac ucgugcgggu ugugaaguuu agcacaaaug aguucacccu ggcuccgcuc     3120

aacgaaggag gcgcccugaa aucuagacac gcggauaagg gcgacccuuu ccgcuaucuu     3180

uaccccuccc cuagcacacu gaaagccugg cgggcuagag agguccgcgu ggaugaacuc     3240

gggcggguuc uggaccccgg auuucccgcc agaaagcgcc ggagagugac ccgcccuggc     3300

cgggcugaug ccgacuga                                                   3318


<210>  3919
<211>  3321
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3919
augcaguaca ucguggccau ugacaucggc gucgcuagcg uuggguaugc cgugauugau       60

aagaccuccg agacagugau cgaagcggga ucuaacauuu uccccgaggc uagcgccacg      120

gacaaucaag aaagacgggg caugcgccag gccagacggc ugaaacgcag agagcggacc      180

cgccucgaug acuuuacaaa gcugugggaa uaccacgggc uugcuauccc ucaggucaaa      240

aagaacgaua ucgugggacu gaaaguuaag gcccucuaug aguccauuuc ucuggacgag      300

guguaccuua uccuguauag cuaccucaag cauagaggca uuuccuaucu ggaagauguc      360

gaggacucua gcguguccgg gagcaccgcu uacgccaaug gacugaaacu caacgcgaag      420

gaacuggaga aaaauuuccc gugcgagauc cagaaguacc ggcuugaaaa cauuggcaaa      480

uaucgcggcc aaauccagau ugaggaugac cagggggaaa agcuggaucu cucuaauguu      540

uuuacaaucg gagccuacac caaggagauc cagcaaauuc uggacacaca gaaaaaguau      600

cacgcugaaa ucgaggauga guucugugaa aaauaccuua agauuuuuac cagaaaacgg      660

aaguauuacg agggccccgg gaacgaaaaa ucccgcacgg acuauggaaa guacaccaca      720

agacugaagg agaauggcga auacaucacc gagaaaaaca uuuucgagaa gcucaucggg      780

aaaugcagcg uguauaagga cgaacugcgg gccgcuucug ccuccuacac agcgcaggag      840

uuuaaucugc ucaacgaucu gaauaaccuu accgucaaug gacgcaaacu ggaagagaac      900

gaaaagcauc ucaucgugga caaggugaaa acgagcaaca caauuaauau gagaaagauc      960

auugccgaug cuaugggcga gaaaaucgag gaguucuaug gcgcccggau ugacaagucu     1020

gaaaaagaaa ucuuucacaa guucgagguc uacaacaaga ugagaaaagc ucuggccgcc     1080

auccauguua auauugagga auuuucccgc gaugaccuug augagaucgg guauauucug     1140

accaucaaca cagacaagga agcuaugaug gaggcguucg auaauagccg gcucgaacug     1200

ccuggagaga uuaaaaacug ucugaucucc cucagaaaga ccaauggcac ccuguacucu     1260

aaauggcaca gcuuuucccu uaagauuaug aacgagcuga uccccgaaau guaucgccag     1320

ccuaaggagc aaaugacacu ccugacggaa auggggguga ugaaaggaca gaccgacgag     1380

uucgaaaaga auaaauacau ccccguggau gucgccagcg aggacauuuu caacccugug     1440

guucggagau cugugcgcau cuccuuuaag auucuuaaug cucugaugaa aaaguacggc     1500

acacucgacg aggucgugau cgaaaugccg cgggauagaa acagcgacga gcagaaaaag     1560

ugggaaaagg agacccaaaa gaagaaugaa aaagagcagg aguauauuga aaacaagcug     1620

gccacaguuu acgauauccg ccugucuagc gccgacuucu ccucucagaa acagcucaau     1680

cugaagcuga agcuguggaa cgagcaagau gggaaaugcc ucuauagcgg aaagaucauu     1740

gaacccgagg accugaucaa acacuccgag cuguuugaaa uugaucauau cauuccucgg     1800

agcaucucuu ucgacgauuc cagaagcaac aaggugcuug ucuaccgcac cgagaaucag     1860

aacaaaggca aucagacgcc cuauuacuau cucacacaca ccaacucuaa gugguccuuu     1920

gaacaguaca aggcucuggu gauggaccug agcaaaaaga aagaguacgg ccucuccaag     1980

aaaaaggucc ggaaucugcu uuauucugaa gauaucacca aggccgacgu gcugagaggg     2040

uucauuaacc ggaauaucaa cgauacaagc uacgcggcuc gcguugugcu caauaccguc     2100

caaggauauu uuagagccaa agagaugggc acaaagguga aaguuauucg gggguccuac     2160

acgcaucaga ugagagugaa caugaagcug gacaaagacc gcgagaaguc uuaugcccac     2220

cacgcugucg augccaugcu gaucgcuuuc agccagcuug gauacgaagc cuaucggaaa     2280

cuccagggcg aguuuauuga cuucgaaacc ggggagaucc uuaauuccga aaaguggaag     2340

gaucaaauga gcgacgaggu guacaaacag uaccuguaua gaaagaaaug gucuaacauc     2400

cgcaaugaga uuacaagagc ggaaaagaac gugaaauacu gguauuacgu ugaucauaag     2460

uccaaucggg gccucuguaa ccagaagauc cgcggaacca gagucuauga cggcaaaauu     2520

uacaagauca acaaacugga uauucggaag aaagaggggg ccgcucuuuu uaagaagcug     2580

gccuucagcg aaaaagaguc ugaccgcgaa aagcuccugg uguauaaaaa ugauaagaaa     2640

acauacgagg accuggugag aaucaugcag gauuacuccg acagcaagaa cccuuuuguc     2700

caauaugaga aggaaaccgg agauuacgug cggaaauaug cuaagaaaca caauggcccc     2760

cgcauugaca aacuuaaaua caucgacggg gagguuggau cuugcaucga uauuagccau     2820

aaguauggcu acgaaaaggg guccagaaaa gugguccucg agagccuggu gccguaucgg     2880

auggacguuu acuacaagaa agaggaacag acguauuacc uggugggcau caagcagucu     2940

gaugucaaau gucaaaaggg agaguaugug auugacgaag augccuacgc gaucaaccuc     3000

guuaaagaga agaugauuca gcagggccag aagcgcuccg accuggaaag ccuuggguuc     3060

gaguuccaac uguccuuuua uaaaaaugau aucaucgagu acgaaaagga cggaaaaauu     3120

gugagagagc gguuccucuc uagaaccaug ccuaagguca aaaacuauau cgaaacaaag     3180

cccauugauc gcgccaagua cgaguggcac gaccggaaac uggugggccu ggggaagacc     3240

aaaagaaucc gcaaguaccg gauggauauu cuuggaaauu auuaccucug caacaaagaa     3300

gaguuuagca aguauuguug a                                               3321


<210>  3920
<211>  3324
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3920
augggcuaca ccguggggcu ggacaucgga gucgccagcg uuggcguggc ugugcucgau       60

gagaacgaca auauugucga agccgugucc aacaucuucg augaggcgga cacaucuaau      120

aacaagguua gacggacgcu gcgcgaaggg agacggacca aacgcagaca gaagacacgg      180

auugaggauu uuaaacaacu uugggaaacc agcggauaua ucauucccca caagcugcau      240

cucaauauca ucgagcugcg caacaaaggc cuuacagagc ugcucucccu ggacgaacug      300

uacugcgugc uccugucuau gcuuaagcac agagggauua gcuaucugga ggaugcugac      360

gauggagaaa agggcaaugc cuacaaaaag ggccucgccu ucaacgagaa acagcugaag      420

gagaaaaugc cuugugaaau ccagcuugag cggaugaaga aguaugggaa auaccaugga      480

gaauuuauua ucgagauuaa ugacgaaaag gaguaucagu ccaacgucuu caccacaaag      540

gcuuacaaga aagagcugga aaagaucuuu gagacccaac gcugcaaugg caacaaaauu      600

aauacgaagu ucaucaagaa auacauggaa aucuaugaga gaaagcggga auacuauauu      660

gggccgggaa acgagaaauc ucgcaccgau uacggcaucu auacaaccag aacagacgag      720

gaagggaauu uuauugauga gaagaacauc uucggaaaac ucauuggcaa guguagcgug      780

uaccccgaag aguaucgggc cuccagcgcu ucuuacaccg cccaggaauu uaaccugcug      840

aaugaccuca acaaucugaa gaucaacaau gagaaacuua cggaguucca gaagaaagaa      900

aucguugaga uuaucaagga cgcguccagc gugaacaugc gcaaaauuau caagaagguc      960

auugaugaag acaucgagca guacucuggc gccagaaucg auaaaaaggg gaaagagauu     1020

uaucacacau uugaaaucua ccggaagcug aaaaaggagc ucaagaccau uaauguggac     1080

aucgauuccu ucacaagaga agaacuggac aaaaccaugg auauucuuac ccugaacaca     1140

gaacgcgaga gcaucgugaa ggcuuuugac gagcaaaaau ucgucuauga agagaaucuc     1200

auuaagaaac ugaucgaguu ccggaagaac aaucagagac uguuuucugg auggcauucc     1260

uucagcuaca aagccaugcu ccagcugauc ccuguuaugu auaaggagcc caaggaacag     1320

augcaacuuc ugacggagau gaacguguuu aaauccaaga aagagaagua cgugaauuau     1380

aaauacauuc cugaaaacga ggucgugaag gaaaucuaca aucccguugu ggucaagucu     1440

auucgcacca cagugaaaau ccucaacgcu cugauuaaga aauauggcua cccugagagc     1500

guugugaucg aaaugccgcg ggauaagaac uccgacgaug agaaagagaa gaucgacaug     1560

aaucagaaga aaaaccagga agaguaugaa aagauucuua auaaaaucua cgacgagaag     1620

gggauugaga ucaccaacaa agauuauaag aagcaaaaga aacugguccu caaacugaaa     1680

cuguggaaug aacaggaggg acucugccug uacagcggca aaaagauugc caucgaagac     1740

cuucugaacc accccgaguu cuuugaaauc gaucacauua ucccuaaauc uauuucccuc     1800

gacgauagca gaucuaauaa ggugcugguc uauaagacag agaacagcau caaagagaau     1860

gacacccccu accauuaccu gacgcgcauu aacgggaagu ggggauucga ugaauauaaa     1920

gcgaaugugc uugagcuccg gagacgcggc aagaucgacg auaaaaaggu uaacaaucug     1980

cuguguaugg aagacaucac aaagauugau guggucaaag gcuuuaucaa ccggaaucuc     2040

aacgacacca gauacgccuc ccgcgugguu cugaacgaga ugcagucuuu cuuugaaagc     2100

cggaaguauu gcaauaccaa agugaagguc auuagagggu cccuuacaua ccagaugcgg     2160

caagaucugc accucaaaaa gaaccgcgag gagagcuauu cucaucacgc uguggacgcc     2220

augcugaucg cuuucuccca gaagggauac gaagccuaua gaaaaauuca gaaggacugu     2280

uacgauuuug agaccggcga aauccuggac aaagagaagu ggaauaaaua cauugaugac     2340

gaugaguucg acgauauccu uuauaaggaa cggaugaacg agaucagaaa gaaaauuauc     2400

gaagccgagg aaaaggugaa auacaauuau aagauugaca aaaagugcaa ccgcgggcuc     2460

uguaaucaga caaucuacgg aacgcgggag aaggauggca aaauucacaa gaucagcucu     2520

uauaacaucu acgacgauaa agagugcaau ucccugaaga aaaugauuaa cagcgggaag     2580

ggcuccgacc ugcucaugua uaauaacgau ccuaaaaccu acagagacau gcugaagauc     2640

cuugaaacau acucuagcga gaagaauccc uuuguugcuu auaacaaaga aaccggagac     2700

uacuuccgca aguauuccaa aaaucauaac ggcccgaagg ucgagaaagu gaaguacuau     2760

ucugggcaaa uuaacagcug uaucgauauu ucccacaagu acggacaugc gaaaaauagc     2820

aagaaagugg uccugguguc ucucaacccu uauagaacag acguuuacua cgauaaugac     2880

accggcaagu auuaccuggu gggggucaaa uauaaccaca ucaagugcgu gggaaauaag     2940

uacguuaucg auuccgaaac guauaacgag cugcuucgga aagagggcgu gcucaauagc     3000

gacgaaaacc uggaggaucu caauucuaag aacauuaccu acaaauucuc ccuguauaag     3060

aaugacauca uucaguacga aaaagggggc gaguacuaua cagaacgcuu ucugagcaga     3120

aucaaggagc agaagaaccu uauugagacc aaacccauca auaagccuaa cuuccagcgg     3180

aaaaauaaga aaggagaaug ggagaacaca cgcaaucaaa uugcccuggc uaagaccaaa     3240

uacgucggca agcucgugac ggauguucug gggaacugcu auaucgugaa cauggaaaag     3300

uuuucucugg ucguggacaa auga                                            3324


<210>  3921
<211>  3324
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3921
augggcuaca cccuggggcu cgacaucgga auugccagcg ugggcuucgg gcugaucgau       60

aacgucgacg gaaagauucu ugaguccggc guuagacugu uuucuagcgc ugaucccgac      120

aauaaccaga cacggcgcuc cucuagacaa gcccggcgcc ucaauagacg gaaagugcac      180

cgccuggaaa gaguggcgca ucuucuggag gaagcugggu ucgagaaggg agaaguccuc      240

gccccuacgc cguaucaccu gagagugaaa ggccugaccg agaagcucag caaacccgag      300

guuuacgccg cucuguauaa ccuuaugaag caucgcggca ucuccuaccu ggaagaugug      360

gagcucgacg aagccgauuc uagcuccguc agccagaaug uggagcugcu uaaggaguau      420

uacccuugcg aaauucagcu ggagagauau gaagaguacg ggcagauccg gggaaucauu      480

gaaacauaca acaaagacgg cgagaaggag ucucucguua auaucuuucc caccggggcu      540

uauaaaaagg aagccgaggc gauuauccug aaacaaggac aguucuaccc ugaacuggau      600

aagguguuuc ucgagaaguu cacagaaauu cugaccguca aacgccccua uuacgugggc      660

ccugggaaca agaaauccag aacagacuau ggaaucuacc gggagaaugg cgagacccuu      720

gaaaaccugu uugauauucu caucggcaag uguacgaucu auccgcagga ggcccgcgcu      780

gccaaauuca gcuacaccgc ucagcuguuu gacuuccuua augauaugaa caauauuucu      840

aucgccgggg aaaagcugac agaggugcag aaaagagaaa uuuaccacca aaucaugucc      900

agcaagaccg ucaacaugau gaagauuauc acaaaaguua ccggaugccu caaggaggag      960

gugacgggcu aucgggugga cucuaaauac aaggcggaau uucauuccuu cgccagcuau     1020

agacaguuuc gcaauaaaau gcuggacuac ggguucgaug uccagacacu gaacguggag     1080

auguucgaca agcucgcugc caagcugacc cuuaacacag aauaucacca aaucauuacc     1140

cagcuggagu cccucaaucu gggacuuucu aaagagcuga ucgaaaagau ugcugauuuu     1200

cggaaaaaga acagaagccu cuucuccaaa uggcacagcc ugucucugaa ggccaugaau     1260

gagguugugg acgaacucug guccaccagc aagaaccaga ugcaaaucuu uucugagcug     1320

ggcggguuca aaagcaauau ugaagucuuu aagggacuua aauacauccc ccuggaugcc     1380

augguggacg agaucuauaa cccuguugug cgcuguucca uucgggaguc uaucaagauu     1440

aucaaugcuc uccugaaaaa guacggcgcg cuggauaagg ucgugauuga aaugcccaga     1500

gacagcaacg agaaagaaga gaaggaucgc aucacaaaac uuaauaagga aaacgacaau     1560

aaaaagaaac aggccauuga gaaggcucgg aacgaguacg gcuucgggga agaggccuau     1620

agacagcaga augaucucaa cacgaagcug cgccuguggu acgaacaaaa acagcggugc     1680

cucuauuccg gagagaucau caagauuuuu gaccugauca aaaauccuca ucuuuucgac     1740

auugaucaca ucauucccaa gagcaucucu uacgacgauu cccugagcaa caaaguccuc     1800

uguuaugcca ccgaaaacag agagaagggc aaucugacac cguaccgcua uuuuaagcgg     1860

aaggcuucuc ugaccuggaa cuacgaggcc uacaaauccu augugaucga acuuuacaaa     1920

gacgggaagg gagaucucag cagaacaaaa cuggagcugc uccuguucga agaggacauu     1980

aauaaguaug agguucggca gcgcuuuauc aacagaaauc uuguggauac ccgguacgcg     2040

uccaaggucg ugcugaacac gcuccaggac uucaugaaag aaaauuaucc uacaaccacc     2100

guugaggugg ucagaggcaa guuuacagcu caacugcgcc ggcauugggg gauucggaaa     2160

aaccgcgaug aaaaucacgc ccaccaugcc aucgacgcuc ugauuaucgc cugcgugccc     2220

caccuuucuc ucuggaagga gcagaaaaag guguucagca ccgaugaguu cagaguugac     2280

gaaacaacgg gcgagaucau ugaacagucc uacaaccagc ugaccuauca agagccuuuu     2340

ucucaguucu uuuaccagcu ggaaaagguc agcaucgauu gucgguacuc cuauaaagug     2400

gaccgcaagu ucaauagaca gguggcugac gccacaauuu acagcacccg gcaaggaauc     2460

gucaaacaga caucuggcga uaaguccuuu acccucgaca gcaacucuaa agagcauuau     2520

gugauugcga agaucaaaaa uaucuacgaa gauguugacg ccaagcgcuu cauggauaga     2580

uauaagaaag acaagacgca guuucugaug uaccaccaug auccccaaac cuucuccauu     2640

cuugaggacg ugaugaaaac auaucccgau aagaaaaacc cuuuucaggc uuaccggcug     2700

gaguacgggc cgauccgcaa guauagcaag aaaggaaaug gcccccagau ucagucuauc     2760

aaguacuaug acagcaaacu cgggcaacac auugaacuga agcaggccaa ccaguccacc     2820

aaaaaggucg ugcugcagag ccuuaacccu uggagaugcg auaucuacua uaauaagaaa     2880

gacaagcggu acauugcugc cggacucaaa uaugcggauc ugucuuacca aaagggcaca     2940

gggcuguacg gcaucucccu ccagcgcuau cagcagaucc ugaacagcga gaaaauugaa     3000

cuuuccaucg agcaaauuga acugcuccug gagggaagac ugaaugaguc uaacuucgaa     3060

uuuuguuuca gccuuuacaa gaaugacuuu auccagugga acgacgagaa uaaccagacc     3120

uuccaauauc gguuccuggc cagaaaucuc uccucuccca accgcauuga aguuaagccu     3180

aucaauaaaa gcaaguacga uaaacaggcu gagggccuga agacgcucaa aaaggggcug     3240

aaccgguuuu auaagaucuc cguggacguc cuuggauacc agcacuucau uaaaaaggag     3300

aaacugaagc ucacauuuaa auga                                            3324


<210>  3922
<211>  3330
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3922
augagccacu acgugcuggg ccucgacauc gggauuaccu ccgucggaua uggcaucauu       60

gaucuggaca caaacgauuu caucgacuac gggguuagac uuuuuaagga aggaacggcc      120

gcuaauaacg aagaucggcg caauucuaga agccggcgca gacugcucuc ccggaaacau      180

acccgccuga uggacaugca gaaggugcuu aaagaggccg gcauuaugac agaaucuuau      240

agcccccugu ccaacgugua cgagaucaga aagaaagggc ucaccaagga acugacaaau      300

gaugagcuga ccgcggcuau ccuccacauu acaaagcauc ggggaucugu caucgagacc      360

guggaagacg augaggccgc cgcuaaagaa acgggcgagc uuaagagcac ccuucacgag      420

aacgcccugc uccuggcuaa uaacaaauuc guuugcgaaa uucaacuuga gcgccugaau      480

acagaaaaca agaucagaaa ucauaaaaac aauuuuaaga ccaaggacua uguggccgag      540

cucaaagaaa uucugaacca ccagaaucug uccgaggagg ucaagaacaa aaucauucag      600

aucguggaac ggcgcagagc guacuaugag ggcccuggga gcuucaacuc uccgacaccc      660

uacggacggu uuaucgaagu uaauggccag gugcaagaga uugaucucau cgaaaagaug      720

cgcgggaaau guuccgucua cccugacgag uucagagccc ccaagaugag cauuucugcu      780

gaucuguuua acauccuuaa ugacuucaac aaucugcucg ugcagggaga gaaacugcag      840

gaagaggaca agaagaaccu ucugaaaauu aucaaugaua agggcuccau cacccuccgg      900

cagauuagca aaaagcugga agugaacgag gacgaaauca aaggguauag aauugauaag      960

aagggaaaag cccuguuuac ggaguucaag ggcuacaaaa uccucaagaa acuguuucuu     1020

aauugcggcc aaucuauuuc ccuggaggac uaugaaaugc ucgaugagau caucgaaauu     1080

gucacaaaga agaaagggau cgacgagcgc aaggaugcuc ugaaagagcu uaagugggac     1140

cugagcgaug acauuaucaa cucccucucu gaaguuaccg gaauuguggg cuaccacagc     1200

cugucccuga aagccauccg ggagcucgau agcgaacugu auaagacauc ucuuaaucag     1260

augcagcugc uccaugagcu gaagauguuc gacaaaaaca gaccuuccua caaggggaaa     1320

aagaauauug uggcggaccc cgaagccauc cugagcccug ucgcuaaacg cgcccagaac     1380

gagaccuuua aggugaucaa ugcucugcgg aaaaaguaug gagaguucga uucuauuguu     1440

guggaaauga ccagagacaa gaacagccaa gagcagaaaa agcgcaucaa cgauucccag     1500

aaagcccucg aaucucugaa uaaggagguc gaccaacugc uccagcagaa aggcuacgau     1560

ccgcagaagg ugaaggggaa aacaaagcaa aaaguucggc uguacaugau gcaggaagga     1620

aagagcgccu auacgcuuca ggagcuggac cuccacagag ugauuaaaga ugacaaguac     1680

accgagaucg aucauauuau ccccaucucc auuagccugg acgauucuca guccaacaag     1740

guccuggcgc uucacagcga aaaucaacag aaaggcaacc ucacaccuuu cauggcuuuu     1800

cacaccggcc gcuucgccga cauggggugu acauggaaua aguuuaaaga gaacgugcug     1860

ucuaauaaga acuauaaaca uaagaagaaa aauaaccugc ucgaugaacg ggacaucaau     1920

aaguucuccg ucauucagga guuuaucaac agaaaucugg uggacaccag cuacgcuaac     1980

cgcguugugc uuaauacgcu gacagauuau uucaaagaca acgaaauuga uaccaagguc     2040

cacaccguga acggaaaacu cacagaccug uuucggaaga gaaucaaucu gaaaaaggau     2100

cggaacgagg acuaccuuca ucacgccauc gaugcccuca uuguugcuuc caucaagaaa     2160

cugcgccugc ucgacggcua ucuggccaag uacggguucg agacccuuua cgaugaaaug     2220

acaggagaga uuaaagaaau ccccgaggac aaggcguuuu ucgaugacaa auauauuaga     2280

uacauccuga aucucaagac gaucuaugag ucuagcaaca uguacuaucg gggcauucug     2340

gaaagagagg aacugaucua ccagccuauu aagaucuccc auaaaauuga uaccaagccc     2400

aaucgcaaag ugucugacga gacaaucuau agcacccgga uuaucgacaa cgaagaucac     2460

cuuaucgaga aguacuccga cauuuacgau ccgaaauuua agaaucucac agaggacauc     2520

cugaacgggg aaaagaauaa auaucugaug gagaagaacg auccucaaac cuucaauauu     2580

cucaaacaga ucauuuuuaa ccacuacaau acguauaagg acagcaaaga auacuauaag     2640

aucgagaaga aaaaggaaaa agagaucauu aagcuggucg gcaucaaccc ccuuucucug     2700

uacaaagagg aaaauggacc uauuaagaag uauuccaaaa agggcaacgg gcccgugauc     2760

augagcauga aauacgauuc ugagaagcuc ggaaaccaua uugacaucac ccccgauucc     2820

gcuaaaaaua agaaagugau ccugaagcag auuagcccuu acagaacaga cuucuauaag     2880

ugcucugaug aaaaauuccg cuuuguuacc gucagauaca aggacgugca cuaucagaaa     2940

agcaucgaca aguacuguau ugauaaagag ugguaucaaa acgaaaagaa gaauaaaaag     3000

aucgacgaga aagccgaguu ccuguuuucc augcaucacg augaacuuau uggcaucaca     3060

cggaaggaga gcgaacaguu cauuuacgac gagucuaaag agaaccacgg ggauccgcgc     3120

uaucaugacg gagugaagcc cgaaauccug aaguuuaccg ccacgaauaa cgauacaaaa     3180

ggcaucauug aggucaagcc ucucuacacc uacuccgcuu auagacugaa acccacagug     3240

cucaauguua agaacaucaa aaaguacgcc accgacgugc uggggaaucu uuaugaaguc     3300

aagcagaacg agcugaaacu cgaguucuga                                      3330


<210>  3923
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3923
auggccgagg gcaagaaaag aaucuggcgg cugggggcug accucggagc caacagccug       60

ggcugggcgg cuauugaacu uuccgccccc gugaauggga agccuaucgc cauucuggcu      120

gccggaucuc gcaucuucag cgauggcaga gacccgaaau ccggggcuuc ucucgccguc      180

gaucggcgcg acgcgagagc caugcggcgc agacgggauc gcuuuaagca gagacaaaaa      240

gcucugauua aguaccuuau cgccgacgga cuguuccccg cugaugaggc cgcgcggaac      300

gccauggcug ccaucgaccc uuaugcuguu cgcgccagag cccucgauga agcucugagc      360

cugcccgagc ucggccgggc gcuguuucac cuuaaucagc gcagaggcuu caaguccaac      420

cggaaagccg acagaaaggc ugaugacgau gccgggaaaa uugccaucgg aauugaccgc      480

acccgggacg cuaucgccga agaugaggcg gacgcuggcg ccgagccugg gucuuggaca      540

uuuggaaagu ggcugcauga aagacgccuc aaaggccaga gcgugcggac gagacugcgc      600

cccgaguccg gggaagaugc caagggagac ggcuacgauu ucuauccuga ccgggcucuu      660

auggaggagg aauuugaugc cauuugggcu gcccaggcgc cgcaccaucc cgacgugcug      720

accaaugaug uccacgacag acuccaugag aucauuuucu accaacgccc ucugaaggcu      780

ccccaggugg gcaugugcag ccugaucccu ggggaucgga gacuccccaa agcucacccg      840

cuguuucagc gccggagacu ucuggaggaa cucaacgccc ugaugaucgu ucggccugga      900

gaaguggcuc agcgccuuac aagagaggag cgggacauuc ugcuccugaa gcugaaagac      960

aagaauaaag ucucuuucgc cacccucaga aaggcgcuga aacuugaucc cgacgcccgc     1020

uuuaacaagg aauccgagga ucggacagac cugaagggcg augaaguggc ugccguuaug     1080

gggagcaaaa ccagauucgg aaaucucugg gugcaccugu cuaucgaggc ucaaugggac     1140

guccuugccc gccuggaugc cguggaaucc gacgcugagg augcggccuu uugugcuugg     1200

cucguggaga cauaugcccu gaccccugaa caggccagag cugucgccaa cgcgccccug     1260

cccccuggcu acgggcgguu cggagagacg gcuacccgca agcucauuga agcccugaga     1320

gacggcaaaa aggaugaccg gguucuugug uauagcgagg ccgugacaga agcuggccug     1380

gggcaucacu cugaucgcag aaccggagag gucuucgagg acgccaaagg ccggccgcag     1440

cuccccuacu acgggguggc gcuggaacgc cauaucaugc cuggaacagg cgaccccucc     1500

gauccugagg aaaugagagu ugggcggcug accaauccca cggugcacau uggccuuaac     1560

cagcuccgca gaauccugaa uguccugauc cgggcuuaug gaccgccugc cgagauugcc     1620

aucgagcucg cuagagaacu gaaacuuaca gacgaggaua agaaacgcau uaaccgggaa     1680

aauaacgaca acagaaucgc cgcugagcgc cggagcgaaa agcugcucgc cauuggccaa     1740

gcggauuccg gggccaauag agcucugcug aaacuuuggg aggagcucaa ccccgccaau     1800

gugcuggacc gccggugcau cuacucugga agacagauca gcauugauau gcuguuuucc     1860

gcugccguug aaguggacca cauccucccu uucgccgcua cccuggauga cagcaacggc     1920

aauaagauug ucugucuucg cgaggcgaac cgggaaaaga gaaagcgguc uccccaugau     1980

gccugggggc acuccgcucg cugggacgag aucgccgcca gagcugccca gcugccucgg     2040

aauaagcgcu ggagauuuga acccgaugcg auggacaaau uccgggacga gggaggcuuu     2100

caagcuagac aucucgugga uacacaguau cuggcccgcc ugagccggga guaccuugcc     2160

acccucuauc cggaaaaggg ggagggcucu ggacacauuu gggucagccc uggcagacug     2220

accgaaaugg ugcgccggaa acuggggcuc aacgaccugc uucccgauca uaauuucgga     2280

ggcggggcug accaggccaa gaacagacug gaucaccgcc accaugcgau cgacgcuguu     2340

guggucgcca uuguggaucg gggaaugcuc aaugccaucg cuagagccuc cggccaggag     2400

ggggcugaag gccgcgagag aaucauuauc cccgagccuu gggacggauu ucgggaugcc     2460

cugcgcgaaa caguuaacag aauuacggug ucucaccgga ccgaccaugg cacagcgagc     2520

aaagccgggc ugccgaaggg aaaggaugcu accgccggcc gccuucacaa ugacacagcu     2580

uacggguuca ccggagacac ggaugccaaa ggcaacucca ucgucgugag acgggugccc     2640

cucagcucuu uuaagaaacu ggccgaccug gaucgcauua gagacgcuga ucucaaggcg     2700

gcccuguauc aacaccuuaa ugggcuggag ggcaaagccu ucgaaaaggc ccucgccgag     2760

uuucauaaac uggaguggca caacuucgga ggcguucgga gaguccgcgu gacagaaccu     2820

cugaccguga uccccaucaa ggaccggaac gggagaauuu acaagggaua caaaggcgau     2880

uccaauuauc gguacgacgu cugggagcuu ccugauggga agugggugac caacuggaaa     2940

aaugaagcug gagaggccau gagcucuguu aucuccaugu uugacgcgca ucagagcucc     3000

gaacccccuc gcccccaccc ggcugccaga aagauucugu cucuccauca gaacgaucug     3060

cucgccaucg agcgggaggg cgaagaccgc aaacugauga gaguggucaa guucggccag     3120

agcgggcaaa uuacacuugc ugagcaccag caggccggag aucugaagaa acgggacgcg     3180

guguccaaug acgaugaccc uuucaaguau uuugccccca ccucuggcgg gcucaaaaag     3240

guuaaagcuc gccaggugag aaucgaugaa aucggacggg uccuggaccc uggcuucccc     3300

gcccgcacag cuagacggaa gacgagaggg uga                                  3333


<210>  3924
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3924
augaccgccc ggacacacaa gaucuggcgg cugggcgcug accucggggc caacagccug       60

ggaugggcgg cuauugagcu uuccgccccc guggauggca aaccuaucgc cauucugcau      120

gcugggucuc gcaucuucag cgacggaaga gauccgaagu ccggcgccuc ucucgcuguc      180

gaccggcgcg augccagagc gaugcggcgc agacgggacc gccugcagca aagacagcgg      240

gcccuuauua aauaccugau cgcugauggg cucuuucccg ccgacgaaaa ugagcgcaag      300

gcuauggcca cgaucgaucc uuaugcgguu agagcccggg cucuggacga agcccugacc      360

cuccccgagg ugggacgcgc ucuguuccac cuuaaccaga gacggggcuu uaaaagcaau      420

agaaaggccg aucgcaaggc cgacgaagau gcuggcaaaa uugcgaucgg gauugaccgg      480

acaagagacg ccauccgcca ggcugaggag gaagccggag ccgagccugg cuccuggacc      540

uacgggaagu ggcuguauga acggagagcu ucugccacag augcgaacgc uauucccagc      600

gugcgcaccc ggcucagacc ugagacagac gagcaugcca aaggagaugg cuacgacuuc      660

uauccggggc gcgcccugau ggaagcugag uuugccgcua ucugggaagc ccaagcggcc      720

caccaucccg augcucuuac caaugagguc cacgaccggc ugcacgaaau uaucuucuac      780

cagagaccuc ucaaggcucc ccagaucgga gugugcacgc ugauucgcgg cgaucagcgg      840

cugccuaaag cucauccccu cuuucaaaag agacggcugc uugaggagcu gaacgcccuc      900

accaucguuc gcccgggcga aauggcgcag agacugacac gggaggaaag agacacccuu      960

uuccugaagc ucaaagauaa ggccaaagug uccuuuguca gccugcgcaa gacacugaaa     1020

cucgacccug augcucgguu caauaaggag ucugaaaaca gauccgaccu gaaaggggac     1080

gagguggccg cugccauggg agccaagacc cgcuuuggca auagaugggu ucaccuuagc     1140

auugaggaac agugggaugu gaucgcucgg cuggaggaag ucgagucuga cgcggaagau     1200

gccgcuuguc gcgccuggcu cgccgcuacg uucgaccuga cacccgagca gauuagagcc     1260

gugaccaacg cgccucuucc ucccggguau ucccgguuug gaaagguggc uacacaacgc     1320

cugaucgaug cccucagaga cggcgagaaa gaugggcggg uccugguuua cagcgaagcc     1380

guggcugccg cgggacuggg ccaucacucu gaccuccgca ccggcgagau cuucgaagau     1440

gcucagggga gaccccagcu gccguacuau ggaguggccc uugagcggca cauuaugccc     1500

ggcaccggga auccugacca uuccgaugag augcgcaucg gaagacugac aaaccccacg     1560

guccacauug gccucaauca acugcggaga aucgugaaca cccugauucg cgccuacggg     1620

ccucccgcug aaaucgccau cgagcuugcu cgggaacuca agcugacaga cgaugacaaa     1680

aagagaauua auaaagagaa caacgaaaau cgccuggccg cggagaagcg gagcgagaag     1740

cuccugacca ucggcagacc ggacacagga gccaaccgcg cccuucugaa acucugggag     1800

gaacugaauc cugccaacgu ucuggaucgg agaugcauuu auaccggcac gcagaucucc     1860

guggacaugc uuuucucugc ugccgucgaa guggaucaua uucuccccuu uacagccacc     1920

cuggacgaua gcaaugggaa caagaucguu uguaugcgcg aggcuaaucg gaccaaaaga     1980

aagcaguccc cuuacgaagc guggggacac acagaccggu gggaggagau ugccgcucgc     2040

gccgcccagc ugcccagaaa caaacggugg cgcuucgaac cugaugcuau ggagaaguuu     2100

aaggacgaaa gcggcuucca agccagacau cucguggaua cccaguaucu ggcgcggcuu     2160

ucuagagagu accuggaaac acucuauccc ggguccggag agggcagcuc ucacaucugg     2220

gucagcccgg ggcgccugac ggagauggug cggagaaaac ugggccuuaa ugaccuccug     2280

ccugaucaca acuuuggagg cggggcugac caggccaaga aucgccugga ucaucggcac     2340

caugccaucg acgcugucgu gguugccauu guggacagau ccaugcucac cgaaaucucu     2400

cgcgucagcg gacuggaggg cgcggaaggg cgggagagaa uuaucauucc cccucccugg     2460

gaugaguucc gcaacgaccu uagaacagcu gugaaugcca ucaccguuuc ccaccggaca     2520

gaucacggaa ccaucagcaa agccggccug ccgaagggga aagacgcuac ggccggccgc     2580

cuccauaacg auaccgcuua cggauuuaca ggcgaaaccg acgccaaggg aaauucuauu     2640

guggucagac gggugccccu ggcguccuuc aagaaacugg ccgaucuuga ccgcaucaga     2700

gauacagacc ucaaggcugc ccuguaccag caccuggagg ggcucgaagg caaggcuuuu     2760

gagaaagccg ugauggauuu cccuaaccug gaauggcauc ccuuugggaa cguucggaga     2820

guccgcguga ccgaggugcu uagcgucauu ccuaucucug accaaaaugg acggauuuau     2880

aaaggcuaca agggggacuc caacuauaga uacgaugugu gggagcugcc cggcggaaaa     2940

ugggaagccg agguugugag cacguucgac gcucaccagc cugauugggu cuccaagauc     3000

cgcucucgga gccccacagc gagaaaggug cucucccugc agcagaauga ccugaucgcc     3060

guugaacggg cucaugagac ccgcgaacuu augagagugg ucaaauuucg gcaaaacggc     3120

gagauuacau ucgccccgcc ucaggaggcc gggucucuga agcgcagaga ugcuaccccc     3180

aaugaagacg auccuuucaa auauuuugcc guggcggcua gcucccucca gaaggccaaa     3240

gcccggcagg uucgcaucga cgagcuggga agagugcucg aucccggcuu cccggcucgg     3300

accgcccgca gaaagacaag aaagagcacg uga                                  3333


<210>  3925
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3925
augagaaucc ugggcuucga cauugggauc accagcauug gaugggccua cguggagggc       60

gaagaacuca aggauugcgg gguccggauc uuuacaaaag cugaaaaccc ccagaaugga      120

gacucccugg ccgcgccucg cagagaggcu cggggcguuc gcagacggcu ugcccgcaga      180

aaggugcggc ugaacgugau uaaacgccuc cuguguaagg aguucgggcu uaaucugucu      240

gauuauuuug ccaacgacgg agagcucccg aaagccuacg agacgaccaa ggauacaaag      300

agccccuaug aacugagauc ccuggcccuc gagcaaaaac uggaaccuaa ggagcuugcu      360

cgggucaucc ugcacaucgc caaacaucgc ggcuacggca auaagcacgc gaaaagagac      420

cucgaggccg aagagaaggc uaagaaagcc gcugaaaagg cuccccagga gaaagaaucu      480

accacaacca acggggauag caagaaagag caggagaagg ugcugaaagc gcuuuaucag      540

aaugaaacac ugcucaagaa guaccaaacc aacuaucaga cgaauaacaa aaccaaugac      600

gagacaaaca auaagaccau uuuccugguu ggaaaauacc uguacaagga auuucaggau      660

aaaggccagc ggauccgcaa cacaaccaau aacuaucaac auacgaugag acaggagugg      720

cucaaggacg aacuggaguu cauuuuuaag aaacagaagg auuucggggc cacauuuucc      780

gagaacuucg aaucucagau ccuugagacc gcuuuuuacc aacgggaccu gaaauccuuc      840

gaagauaagg ugggaaaaug cgucuuuuau gagaaugaaa acagagcccc uaaggacagc      900

cucagcgcua uggaguucgu ggcccugaca cgcauuauca auacccuuaa gaaccuggag      960

aaaaagucua aaaaucucgg cauuggggaa accuacggaa aggacaaaau ccaggagauc     1020

cugaagauug uucuggauaa gggcgaaaug uccuauaaag agcuccggaa gauccugaaa     1080

cuuaacgaga gcauucuguu cgccaaggac ucuaaacucg auuacacaaa gggcaugcag     1140

gaagcugaga aagcgaaguu uaucgaacug aagaaucuua aagccuucaa gaaagcuaug     1200

ggaggggacu uuuccaaguu ccccagagag gaacuggaua aaauugccac ggacaucgug     1260

cucaucaaga gcaaggagaa ccuggccaaa aagcugcaaa auuauucugc ucucuccaaa     1320

gagcaggucg aagcccugag caaccuuaau uuugcgaagc acauugaucu gucccucaaa     1380

gcucuggaca agauccugcc uuucaugcgc gagggccuuu cuuacgauga aguggugaag     1440

aaagucgggc uccaggagca ucggaaguac aaacagaagg gagaauuucu gauuccgcug     1500

aaagaguaug agcccuaccu cgccaacccu guuguggcca gagcucugag cgaauaucgc     1560

aaggugcuua augcccugcu ccggaaguac ggcaacgucc acaaaaucca ccuggaguuc     1620

accagagaag cugggaagaa uuaugacgag cgcaaaaaga uugaaaaaga gcaacgggag     1680

cauuuugaag ccaaccagaa ggcgaucaaa cagagagaga uccugggacu ucccauuaac     1740

ggcgccaaua uccucaagau gaagcugugg auugaacagg gcgaguguug cguguacucc     1800

ggggagaaaa ucacaagcaa gcacuucuuu gauucuaacg cucugcaagu ugaccauauu     1860

uauccuuacu cccgcagcuu cgacgauucu uacaugaaua aagugcucgu cuuugccaag     1920

gaaaaccaga auaaacugaa caagaccccc uucgaggcuu ucggaagcga caaggaaaaa     1980

ugggaucgga uccuuucccu gucugccaag cucccgaaac agaagcagag acggauuagc     2040

aauaaagacu uuaaggauaa ggagcugggc uucaucgcgc gcaacaucaa ugacacaggg     2100

uauauugcca gacuggcuuc ccaauggacc aacaauuguc uuaaauuuga agaucuccgg     2160

gagaacgagg ugacgaucgc cggagaaaag ggcagcaaaa aucaccugga gguuauuucu     2220

gggcagcuga cauccaugcu cagacacuac uggggccugg gagacaagga ucgcaacaau     2280

caucuucacc augcugugga cgccgucauc auugccuaua ccaacgcuaa aaccaucaag     2340

gcguucgccg auuuuaagaa aaaccaggaa cagaaucggg cuaaguucua cgccaaacaa     2400

aucagcgagc aggaauauaa gagacagcgc aacccuuuug agcccugcgc caauuucaga     2460

caacgggugg aggagaaaau uaacaagauc uuugucucua agccuccccg caaaagagcu     2520

cggggcgccc ugcacaagga aacauucggg uccauuuguc ucgacggaaa uaacaauugg     2580

guggcgcgcu uuaaaaucaa gagcaaaaag gaacaggagg auauuaccaa agaaaaggcu     2640

acaaaggcca aauacaagga cgaguucaaa ggcgaggaaa acaucagaaa ggcccugaaa     2700

cuggggaaga uccggcgcau uaauaacacg cagaaggaca ucgcuauugg agauaaaauc     2760

guuaagaaug agaccauggu gagagucgac auuuuuaaag auaagaaagg caaguucuau     2820

gccgugccca ucuacacaau ggacuucgcg cuugggaucc ucccuaacaa ggcuguugcc     2880

accggcgaag augaggacgg agugauuaaa gauuggaucg agauggacaa gacaaaauac     2940

gaauuuugcu ucucccuguu uaaggaugac cugauuaaag uccggaagaa gaaaaugaag     3000

gaggccgaac ucuguuauuu cgugggcuuu gauaccgacg ggucucgcau cauuguggcu     3060

aaacauaaua acgauuacca gacgaugacc gagaaugaau gcaagcuguu cacacuuaaa     3120

gagaagaaaa agaagcagaa aacccugaac gacgaggaag acaaccaaga gagaaucgaa     3180

acagaguaua agauuaucgg aggccggacc gggauccaga aucucgaaac guuugagaaa     3240

caccagguua gcccgcuggg agagguccaa aaggccgagu ucgaagauag acaggaaauu     3300

aaacugaagu ccucucccaa gggcaaagac uga                                  3333


<210>  3926
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3926
augagcgaga aguacagacu gggccucgac guggggacca acucccuggg augggccguc       60

aucggccuua aagaagaugu ucccucuagc gugcuggcua caggggugcg gauuuucucc      120

gagggacgcg acgccaagac gaaaucuacc cucaaggcga gcagaacaga aaaacggucc      180

gcucgcagac ggcgcgauag auuucugcag cggcaaaccu uccuuaucuc ugagaugguc      240

aagagcggcc uguuuccuaa ggacauugug gaacagaaaa aucucgagcu gcuggauccg      300

uaugagaucc gcuucaaggc ccucacagaa cagguucccc ugcaccaucu ugggagagcc      360

cuguuucaca uuaaccagcg gcgcggauuc aaauccagca gaaaggacaa aucugaggaa      420

accacauccg gcguggucag caagucugug cgggcucucu acgaggagau gcaacugauc      480

aauccugccc ccguuaucga ugacccuaag gcucuuucca aagaagaacg cagagaaauu      540

cgggugcagg aggccagcuc uagagaagag gcgcugguca cccucgccga gcagaagggc      600

gugacguuug gggcuuuccu guaucgccgg cugcaguccg gaaaaagcgu gagagcccgc      660

aaggaugcug acucccaacu cuacgauguc uaucccaccc gggaacugcu uaaagacgag      720

uacucuaagc ugaugcagca ucaggcccag uauuacccua acguuaucaa ugaagcgcuc      780

auuagcagac ugcacgaugu gaucuuuuuc caacgcaaac uuaaggagca ggaacggggc      840

uuuugcacau acuauccguc cgagagacgc accuuccggu guaugcccag cuuucagcaa      900

uacagaaugg ugcaggagcu gaacgcccuc gaaugggucg acgcugccgg gaagcauugc      960

cugcgcgacu auccugaguc ucgggaucug auucucuccg aacuggagaa uaucagcaca     1020

aaagcuggag aguucaccuu cggcaacaug aagaaaauuc uuaagaaaau gggggugauc     1080

gacggagagg uuucuuuuaa uuucgagggc cccaagagaa agggcuuuca ggggaacgug     1140

acgagccggg aaaugcgcca cgaggauugu uucggaacaa gaugggacga acugucccuc     1200

gaugagaaag acgauaucau ugacaagauc aaugaugacc accuggauga cgaggacaug     1260

gaacgguacc uuauugauac cuauggccug ucuagcuuuu ccgucgggaa cgccaucaau     1320

gccagacucc uggagggaac agcuaacgug agccgcaaag cggcccggac ccuguacgaa     1380

accaugcucu cugcuaauug ccugcagucc gacgccguua uugccgugag cgagcaagau     1440

gacacauucg ucaacccuua uucuagaacg ggccgcgggg aacuucuguc cgagcucccc     1500

uacuacggag aggcuuuuca ggauggcaga cauaucaucc cgggcaagcg ggaaccuaaa     1560

gugagccacg accagcugaa guucuauggg ggagucucca accccaccgu gcauauugcc     1620

cugaaucaga uccgccuugu ugugaacgag auuaucaaga gauacggccu cccuucuagc     1680

auuuccaucg aacuggcgcg gaaucugccc gaggggcaag auggacgcag aaaaaucgaa     1740

aagaugcagu cugagaacca gaaaaauaac gagcgguaug accagauucu cacagaaaau     1800

ggccaaagcc ccaaccgcga uaauagacug cggcuugcuc ugugggagga acucggggag     1860

ggcccuaccg accgccugug ugucuuuaca ggaaagcuga ucgaacuugc cgaucucuuc     1920

aacggcuccg uggagauuga gcaccugauc ccguuuagcg uuucucugga cgauuccaaa     1980

gccaauaaga ccgugugcug gaaggaagcu aacagauuca aagggaauaa gacgcccuac     2040

gaggccuuug cuaacaauag cggagacuau ucuugggaag agauuaugga gcgggucaga     2100

gcccucccua acuccaaaca guggcgcuuc caggaagacg cgcugaagau cuggaacagc     2160

gaucacucug acuucagcgg ccggcaucuu aaugauacaa gauacauuuc ccgccuggcc     2220

cgggaguauc ucgaaaacau cugucccuuu aauaaaaucg acguggugac cgggagacug     2280

accgcucugc uucgcggaca cuggggccuc aacaauauuc ugcgggggca uaacgagccu     2340

gaaacagcca cccccaagaa aaugagagau gaccaccgcc aucacgcuau cgaugccguu     2400

gucauugcga ugacaagccu gacgauccuc cagaaggugu cuuccgccgc uggcaaggcc     2460

gaggagcugg acgugggaaa acuuuucgcu aaggaugaaa gcggccgguc cgucauugac     2520

ccgugggaug gguuuagaga cgagguggcc gaaaaaguuc ggaauaucau cgugucucac     2580

aagaccaaac gcaagacaau uggaaacagc ggcaagucca ccgauagagc ccugcauaau     2640

gagacagcuu acgggcucau cgacggaccu gacgaaaaag gccuguacuc ugucgugacc     2700

acgaagccca uuagcuauuu cgauaaccgg gacaaaaucg agaagauucg cgaugagaaa     2760

cugagagacg auuuucuuac cgcgugggac ggggaaggcg auaauaaggc cucccaacuc     2820

gcuguucugg acaaggccuc uggacugaac gugaaaaagu gccgguacgu cgagcgcaaa     2880

aaggugaucc cuaucaaugg ccccgauggg cagccuuaua aagccuacga cggaaacagc     2940

aauuggggca uggaaauuua ugaguucccc aacgggcacc cgaaggaaaa gaaauggaac     3000

ggaguuguga ucuccacaua cgaugcuaau aagagcgagu uuaaaccugg cgagaccuuc     3060

agaccccauc ccgccgcgaa gcucgucaug cggcugcaca uuaacgacau cguggaagug     3120

cagaaugacg gggagccuau ucuuuauaga guccaacuga ucucugcugg cacacucacc     3180

guuuccccgc ugcacgaagc caacguggau agccgcaauc gggacaaaca ugaugaguuu     3240

aaguacaaau cucuguccgu gacgcccaug cagaagaugg gagccaagaa agucaacauc     3300

agcccuacag gccuuaagaa uuacucuaga uga                                  3333


<210>  3927
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3927
augggcauca uuaucgggcu ggacauugga aucgccagcg ugggcauugc ugucguugau       60

aaggacaccc ucgauaucaa augcguggug uccgaccugu ucgauucugc cgacgcgagc      120

aagaacaucg agagacggaa uaaccgccag gcuagacggc uuaaucgcag acggaaaaca      180

cgcauugggg auuuuaacac gcuguggguc uccauguucg gagaacuccc cgaggaagug      240

gacucuaaug uucugcuucu gagaaacgcc ggccucgaug agcaacugac ccuggacgaa      300

aucuacugug ugcucaagua uaugcugaaa caccggggga uuagcuaccu ugaggaugcc      360

cugaaugagg aaaacgucac aggauccuau cagaagggca ucgcuauuaa ucagcgcgag      420

ucugaauaca ugcucccuug cgagauccag cuggagagau uucggaagua uggccaauac      480

cgcggggaau gugaggccga aaacgagaau ggagaaaaga uuacccuuag caacguguuc      540

augacaaauu ccuauagaaa ggagaucgac aaauuucuga acacccaggg caaguacggg      600

auccucaauc agaaauucau ugaugaguac cugaagaucu uuaaccggaa gcgcaaauau      660

uacgaaggac cgggcaacga aaagagcaga acagacuaug ggaaauacac cacgggaaag      720

gaccuggaug gcaaauauau uaccgaaaag aauaucuucg agaaacucau uggcaagugc      780

ucuguuuaca aggaggagcu gcgggcugcc ggggcguccu auacagccca ggaguuuaac      840

auccuuaaug accugaacaa uaucaccgug aacaauaaaa agcucacaac ccaacagaaa      900

aaggauguca uugaaaucgu gcagaacagc gacagaauua auauggagaa aaucauuucu      960

uccuguaucg gagaaaagau cgagaagauu gaaggcgcuc gcaucgauaa aaacgagaag     1020

aauauuuacc auacguucga gcaguacaac aaaaugcgga agaucuuugc cgagaaaaau     1080

uaugacauua aggaacugac aaucgaacaa cuugaugaga uugggagaau ccugaccauc     1140

aacacagagc gcgaaggaau uaaggacgcu aucauggaga gcgaacuccu gcaccugaau     1200

aacgagcaga uuaaccuccu gaucgaacuu cgguacaaaa auucuucccu guucaccaag     1260

uggcagagcc ucuccauuaa agccaugaga gagaucaucc ccgaucugua ugaccgcaac     1320

gugaaucaaa ugaacauucu ugcggagcgg ggcgucuuua aggauaaaau caaucuguuc     1380

gaaaacucug uuuaccuccc uaaggagccc gugcuggaag agauuuauaa uccugugguc     1440

agacgcagca ucuccaugac cauugacgcc cugaacgcuc ucaucaagaa auacggguau     1500

cccgaagaca ucgugauuga gaugccucgg gauagaaaug acgccgagca gaaggaucgc     1560

aucaaaaagu uccagaaaac acgggaaaac gagcugaaga auauuaaaaa gaagaucaaa     1620

gaagaguacg gaauugaaau cacggacgag uacuuuagaa agcauaaagg ccuugagcug     1680

gaacucaagc uguggguuga gcagaacgaa aaaugcccgu auagcggcaa guacaucaau     1740

gugcaagaua uucuguauca ccaugagcuu uucgagaucg accacauuau cccccucucu     1800

auuuccuacg augacagccg gaacaauaag guccuggugu auaaagaaga gaaccaggau     1860

aaggggaacc gcaccccucu gauguaccuc ucuacaguua auagacagua ugaccagcac     1920

gaauuuauga acuacgugcu gagcaccaaa acacuucccc ggaagaaaag agagcugcuc     1980

cugaauuacg aagauaucac caagauugac guccugaagg gauucaucaa ccgcaaucuu     2040

aacgauacgc gguaugcuuc cagaucugug cucaaugcca uccaacuguu uuucggcggg     2100

aaagagugug acacaaaggu ccgcgugauu agaggagccg uuaccaacca gcugcggauc     2160

aaacucaauc ugccuaagga ccgcgagaaa agcuacuccc aucacgcuau ugaugcggcc     2220

aucaugugcu auagccagau gggccuuaug aacuuuagaa auauucagaa caaguucauc     2280

gacuuugaaa augagaccua ccugucugau gaacgggacu ccuguuucga uaacaaguau     2340

uacgacgagg cucucuuugg gcaagugcug aaaaugcgca agaaucugcu uaaagccgaa     2400

ggcaagcccc agagaagcgu ccagucuaaa ggaguguucg augaggagga aaagaacaac     2460

ggcaucguuc gguaucauca caaaguggac aagaagccga aucgcgggcu cuccaacgcc     2520

agcauuuacg gaacaagaaa aaucgaugac aagauuuaca aaaucgucgg cuaugauauu     2580

cggaauaaca aggacgcuga ggccaucauc aauauuauca aaaaggggaa guccgauaac     2640

uuucugaugg cgaaacacga cccugaaacc uucaagaauc ugauggagau uuacgaacag     2700

uauaaagagg agaagaaccc cuuccucgcu uacgaacaag agacaggaga cuauauccgc     2760

aaauacgcca agaaucauaa cggcccuauu aucaagaaaa uuaaguaugu ggauucugaa     2820

guggggagcu gcaucgacau cuccgccaau uacggcuaca aaaacggaga uaagagaguu     2880

auuauggaca cgcugacccc cuaucgggcu gaugucuacu auaauuacaa agacggcaca     2940

uauagaauca uuggggugaa guacgccgau uuuaaguauu cuaacgcuaa auacgugcuu     3000

gacgaggagg ccuacgcgcu gauccucauu aaggaaggac ugcugaaaau cggcgauagc     3060

cuugagaaua ucuccagcuc uggguaugaa uaccgccucu ccuucuauaa gaacgacauu     3120

aucgaguaca ccaaaaauga uauugucuau aaggaacggu uucugagcag aacaaugccc     3180

aaggugaaaa acuacaucga gaccaagccu auuucugccg cucuguauga gaaacgcaac     3240

ccgaucggac ucacgaaugc caccaacauc uguaagauuu acacagacgu ucugggcaau     3300

gaaaccaaaa ucuacaagga gaaauucaaa cuuuccgugg acuga                     3345


<210>  3928
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3928
augaacuaca ccgagaagga aaaacuguuc augaaguaua uccucgcccu ggacauuggc       60

aucgcuagcg uggggugggc cauucuugau aaagaguccg aaacagucau cgaggcggga      120

ucuaauauuu uucccgaagc uagcgccgcc gacaaccagc ugagacggga uaugcgcggc      180

gcuaagagaa auaaccggcg ccucaaaacg agaaucaaug acuucaucaa gcugugggag      240

aacaauaacc uuuccauucc ucaauuuaag ucuaccgaga ucguugggcu gaaagugcgg      300

gccauuacag aagagaucac ccucgaugaa cuguaccuga uucucuauag cuaccugaag      360

caccgcggaa ucuccuaucu ugaggacgcu cuggaugaca cagugucugg cagcuccgcc      420

uacgcgaaug ggcucaaacu gaacgccaag gagcuugaaa cccauuaucc gugcgagauu      480

cagcaggaaa gacugaauac aaucggaaaa uaccggggcc agagccaaau cauuaacgag      540

aauggcgaag uccucgaucu gucuaacgug uucaccaucg gggcuuaccg caaggagauu      600

cagagaguuu uugagaucca gaagaaauau caccccgaac ugacggacga guucugugau      660

ggauacaugc ucauuuuuaa ucggaagcgc aaauauuacg aaggcccugg gaacgagaag      720

uccagaaccg acuauggacg guucacaacc aaacuggaug ccaacggcaa uuacaucaca      780

gaagacaaca ucuuugagaa acuuauuggg aagugcagcg uguaucccga cgagcugcgc      840

gcugccgcgg ccucuuacac cgcucaggaa uacaaugucc ucaacgaucu gaauaaccuu      900

acgaucaaug gaagaaagcu ggaggaaaac gagaagcaug aaauugugga gcggaucaaa      960

uccagcaaua caauuaacau gagaaagauc aucucugacu guaugggcga gaauauugau     1020

gacuucgccg gcgcucgcau cgauaaaucc gggaaggaaa uuuuucacaa auucgagguu     1080

uauaacaaga ugcggaaggc ccuccuggaa aucggaauug acaucagcaa uuacuccaga     1140

gaggagcugg augaaauugg cuauaucaug accaucaaca cagacaaaga ggccaugaug     1200

gaggcuuuuc aaaagucuug gauugaucuc agcgacgaug ugaaacagug ccugaucaau     1260

augcgcaaga ccaacggggc gcuuuucaac aaauggcagu ccuucagccu gaagauuaug     1320

aaugagcuca ucccugaaau guacgcccag cccaaggagc aaaugacccu gcuuacagag     1380

augggaguca cgaaaggcac ccaggaagag uuugcugggc ugaaguauau uccuguggac     1440

guggucucug aagacaucuu caacccgguu gugcggagau ccgugcgcau cagcuuuaaa     1500

auucucaaug ccguccugaa gaaauacaag gcccuggaua caaucgugau ugagaugccc     1560

cgggacagaa acucugaaga acagaagaaa cgcaucaaug auagccaaaa gcucaacgag     1620

aaagaaaugg aguauauuga aaagaaacug gcuguuaccu acggaaucaa acuuuccccu     1680

ucugacuuca gcucccagaa gcagcugagc cucaagcuga agcuguggaa ugagcaggau     1740

ggcaucuguc uuuacucugg caaaacaauu gaccccaacg auaucauuaa uaacccucaa     1800

cucuuugaaa ucgaccauau uaucccccgg uccaucagcu ucgaugacgc cagaucuaau     1860

aaggugcugg ucuaucgcuc cgagaaccag aaaaagggga aucagacccc guacuauuac     1920

cugacgcaca gccacuccga guggucuuuu gaacaguaua aagcgacagu gaugaaccuc     1980

agcaagaaga aagaguacgc uauuucccgg aagaaaaucc aaaaucugcu uuauucugaa     2040

gauauuacca agauggacgu ucugaaagga uucaucaaca gaaacauuaa ugauaccagc     2100

uacgccuccc ggcucgugcu gaacacaauc cagaacuuuu ucauggccaa ugaggcugac     2160

accaagguca aggugauuaa aggcagcuac acacaucaga ugcgcugcaa ucugaaacuu     2220

gacaagaaca gagaugaguc uuauucccac caugccgucg acgcuaugcu caucggguac     2280

agcgaacugg gauaugaggc cuaccacaag cugcagggcg aauuuaucga uuucgagacg     2340

ggggaaauuc uccggaaaga caugugggau gagaauaugu cugacgaggu guaugcggau     2400

uaccuguaug gcaagaagug ggccaacauc agaaaugaag uugugaaagc ugagaagaac     2460

gucaaauacu ggcacuacgu gaugcgcaag uccaaucggg gacuuuguaa ccaaaccauu     2520

agaggcacac gcgaauauga cgggaaacag uacaagauca auaaacugga uauuagaacc     2580

aaggagggaa ucaagguuuu ugccaaacuc gcuuucagca agaaagacuc ugaucgggaa     2640

cgccugcugg uguaucuuaa cgacagacgg acauucgaug accucugcaa gaucuacgag     2700

gacuauagcg augccgcgaa uccuuuuguc caguacgaga aagaaaccgg cgacauuauc     2760

cgcaaguauu ccaagaaaca uaacgggccc agaauugaua agcugaaaua caaggacgga     2820

gaggugggcg ccuguaucga uauuagccac aaauacgggu ucgaaaaggg cucuaagaaa     2880

gugauccugg agucccucgu uccuuaucgg auggacgucu acuauaagga agagaaccau     2940

agcuacuauc uggugggagu gaaacagucc gauaucaagu uugagaaagg ccgcaauguc     3000

auugacgaag aggcuuacgc cagaauccuu gugaacgaaa agaugauuca acccgggcag     3060

ucucgggcug aucuggagaa ucucggauuc aaguuuaaac ugagcuucua uaagaacgac     3120

aucauugagu acgaaaaaga uggcaagauc uacacggagc gccugguuuc cagaaccaug     3180

cccaaacagc ggaauuauau ugaaacaaag ccuaucgaca aggccaaauu ugagaagcaa     3240

aaccuugugg ggcucggaaa aaccaaguuc aucaaaaagu acagauauga cauucugggc     3300

aauaaauacu cuugcagcga agagaaguuu acauccuucu guuga                     3345


<210>  3929
<211>  3348
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3929
augagcgcca ccuacacaau cggccuggac auugggaucg cuuccguggg auggugcgcc       60

uucggcgaga cgcacauucu cgaucugggg gucagagcgu uugacaaggc ugaaaccgcc      120

aaagagggag aaucucuuaa ccuggcccgg cgcaaugcua gacucaugcg gcgcagacug      180

uuccggcgca cauggagacu uaagaaacug gcccggcagc ucaagagcga gggccugauc      240

gcugauacca aacuguuuaa gcccgaacuc ccuuucucca agucucugug gcaacuucgc      300

guugacgggc uggauagacg gcucaacgac gaggaguggg cccgcgugau uuaucaucug      360

uguaaacaca gaggauuuca uuggaucagc cgggcggaag ccaagcaggc ugagggcgau      420

uccaaaucug aaggcgggaa ggugaaacag ggacuugccg gcacagcuaa gcugauggac      480

gagaaggggu accgcagcgc cgcggagaug guccucgccg aguucccgca ggcucaaaga      540

aauaaacagg gagaguauca gaaggcccug ucccgggaac ugcucgcuaa agagcuggcc      600

cuucuguuug cccagcaaag agaauacggc aaccuccaca cccccggggc ucuggagaca      660

gcgauccuug gaaauggcga uaagaaaagc ggccuguucu ggcagcagaa gccugcccuc      720

gcugggaccg accugcugaa aaugcucgga acgugcaccu uugagaaggg cgaauaucgc      780

gcccccaagg ccucuuucac agcugagcgg cauguguggc ugaccagacu uaacaaucug      840

cgcauuguua cagauggggu gacccggcag cucacggaac aagagagaca gcugugucuu      900

ccucugcccu accgccaggc cggagaccuc acauauaaac aacugggcuc cgcgcuggcu      960

aaggccgggc uccuggaagc cggaggcuuu aaauucaccg gccuugcuua cccuacagag     1020

gcccagaagg cugaggggaa agccaaggau ccggaaagcg cgacccuggu caagaucccc     1080

gccuggcacg agcuccggca gacccugaca aaaggaggcc ugaacgacga augggagaaa     1140

cuugcugggg acgcccucug cggacagccu gaaauuuuug auaagaucgc uugggugcug     1200

ucuguuuaca aggacgauga cgaggugaga gccgagcugg cgaaacucgc ccugcccaau     1260

ccucaagcuc uuacggaagc ccugcucaac auucgcuucg auaaguucca cucccugagc     1320

cugaaggccc uucgggccau cgucccccau auggagcagg gccucagaua ugacgaagcc     1380

ugugugcagg cuggguacca ccauucucag cugcugaaag ugggcgaggg agagcacaag     1440

uaucucccgc cucuguacga uggccgcgac ggggauggac ggaugauuuu ugcggaagac     1500

gccgauaucc ccagaaaucc ugucguucuu cggucccuga accaagcucg caaaguggug     1560

aaugccauca uuagacggua uggcagcccc uccgccgucc acaucgagau ggcuagagac     1620

cucucucgcc ccuucgacga acggaacaag auuaaaaagg aucaggacga guaccaggcc     1680

agaaaucagc gcgauagaga acaauuuagc gagcuguucg ggcauccucc gaagggacug     1740

gaguuugaaa aauggcggcu uuaucgcgag cagaacggcc agugcgcgua cucccagaga     1800

ccccucaccg cuagcgggaa cguggccgaa aucuuccugg acggcucuac agagauugau     1860

cacgcccugc cuuacucccg gagcuuugac gauucuaaga auaacaaagu ucucgugcug     1920

acccgcgaaa auagagacaa gggaaaccgg acacccuaug aguaccuugg cggggcugag     1980

aauagcgaau ccuggcaacg cuucgucucu uuuguggaga gcaacaaagc cuauagacug     2040

gcgaagcgga cccgccuccu gagaaaagau uucggagcua aggaauccga ggaauuucgg     2100

gagagaaauc ugaacgacac gcgcuacguu ugucgguucu uuaagaauua uguggagcag     2160

caucuuaaac ucgccgaagg cagcgagaca aagagaugcg ucguggucuc ugggcagcug     2220

accaacuucc ugcgcgcccg guggggacuc cugaaaguga gauccgauag cgaccgccac     2280

caugcucuug augccgcugu uguggccgcg uguucucacu ccauggucca acggcugagc     2340

aauuacucca gaacccgcga acucgaacag gugcgggacg gcuucguuga caucgagaca     2400

ggggaaaucg ugaacccugc caugcaccag cagcugagag agcauuuucc cgauccgugg     2460

ccuuauuucc ggcacgaacu ggaggcucgc cuuaagauug acucucccga acugcucaga     2520

gaggagaugg cccggcuggg cagcuacucc gaagaggaac ucaaaguccu gaagccucuu     2580

uuugugucuc gcgcucccca aagacggaau ggaggcgccg cccauaagga uaccaucuac     2640

gggcagccug agaaacugaa gaaaaacgga agcgugacac agaagguugc ucucacgucc     2700

cugacccuga aagacauuga ucuucuggcg gacccccaca gaaaugccaa gcucuauggc     2760

gcuauccgcg aacggcugga ggcccacggg ggaaagggcg auaaagccuu cccggcuaca     2820

aacccucuca gaaagcccga caaagagggg aaccccaccg gcccuauugu ccgcacagug     2880

accaagguga ucgauaaacu gagcggaauc ccgguccggg gcgggauugc caagaaugac     2940

acgaugcuua gaguggaugu uuuuaccaag ggaggcaaau uccaucuggu gcccgucuac     3000

gugcaccauc gcguuacagg gcucccuaac cgggcgauca uugcuuuuaa ggacgaagau     3060

gaguggaccc ugaucgacga cucuuucgau uggugcuuuu cccuguaucc caaugaccuu     3120

cugagaauua aacucaagaa agaaagccac ucuggauacu auuccagcug uaaccgcucu     3180

acaggcgccg ugaaucugug ggcccaugau agaaacguca augugggcaa ggacggguuc     3240

aucgagagca uuggaguuaa aauggcugug uccaucgaaa agcuccacgu cgauacccug     3300

ggcaacaucu acccugcucc cccugagaag cggcgcgggc uugcguga                  3348


<210>  3930
<211>  3351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3930
augagccaga accagaaucc caacacccag ucccaccuga auccuacaac gcagucuagc       60

acccagucca acccgcaacc cacaacccag gagucucuca agauccuggg cuucgacauu      120

gggguggcca gcaucggaug ggcuuuuguc gaaggcgggg agcuuaaaga uugcggaguu      180

agaauuuuca caaaggccga aaauccuaaa gacggcuccu cucuggcgcu cccccggcgc      240

gaggcuagag gggugcggcg cagacuggcc cggcgcaagg gaagacuuaa cgcccugaaa      300

gugcucaucu guaaggaauu uggccugaau cggagcgauu accuggcuac cgccggcgag      360

cucccuaagg cuuaugccac auccaaagag accaagagcc ccuaccagcu gcgcgcggaa      420

ucucuugaga gaaaacuguc cgccgacgag uucgcucggg ucauucucca uaucgccaag      480

caccgcgggu auggaaacaa acaugcuaag gaaagcucug augacgauuc cggcaaggug      540

aaaaaggcca ucagcgagaa ucagaaaguu augucugaaa agggguacac gaccgcggga      600

caauaucugu acggcgaguu uuaucagaaa gccagaaacu ucuccgaagc ugagccuaag      660

agcccgaaaa augcccgggg gacacaggaa uuuaagaacg ugcgcaauaa guccgagaac      720

uacgcuagau gccuugccca gucugagcug caaaaagaac ucgagauuau cuucgccaag      780

cagcgggaau uuggauucca guuuagcgac aaaaagcaca aaaccauuaa ugcuaacggc      840

aagcugaagg agcuggauuu cgcggacgcc guccucgaaa ucgcuuuucu gcaaagaccc      900

cuuaaauccu ucgccgauaa ggugggcaaa uguacauucu acgaggacaa gccucgcgcc      960

cccaaagaua gccugucugc uauugaguuu guugcccuca cccggaucau uaacacgcug     1020

gcgaauauca caaagagauc cggggaagug uauagcgcug acaagguccg cgagauccuu     1080

ucuauugugc uggacaaagg agaaaucacc uacaagaacc uccggaaagu gauugcccug     1140

gaugagagac ugcaguuccc uaaggacagc aaacucgauu augccaagga agacgcugag     1200

aaggccaaag cuaucgaguu uuccaagcug aaagccuucc gcaaggcgcu uggcgggucu     1260

uuugaaggau ucauucgggc cgagcuggau agcaucgcua cagacaucgc ccucauuaaa     1320

uccaaggaug aacugaaagc uaaacuugcc aauuaccaga gccugaacga cgagcagaga     1380

gagaagcucu cugaacuguc cuuugauaag uucaucgacc ugagcuuuaa ggcgcucgag     1440

gccauucugc cccuuaugcg cggcgauaaa gacgacaaga ucuaucggua cgaugaggcu     1500

uggagagaag ccgggcuggu cgaaguugcu aaaaagaccc aaaagggaga cucucucccg     1560

ccucugauug aguaugagaa agaucuggcc aaucccgugg uggcccgcgc ucuugcggaa     1620

uaccggaagg uccucaacgc ccugcugaaa aagcauggca aagugcacaa gauccaccuc     1680

gaguucacca gacaggcugg cguuuccagc aaggaacggg gagagaucau ugccgaacag     1740

aauaaacgcc ugaaggccaa cgaggaggcu agaaaacggu gcgaagagua cggacuuauc     1800

ccuuccggca agaauauucu gaaacucaag cuguggcagg aacaagggga guuuugugug     1860

uauucuggaa agaaaaucac acagagcgac cugagagaug aauccacgcu ucagauugac     1920

cauaucuacc ccuauucucg cagcuacgau gacucccaga acaauaaggu ccucguguuc     1980

accgaugcca accaaaauaa acugaaccag acacccuaug aggcguuugg caaggacauu     2040

agcaaaugga aucagauccu ggggcggauc gagaagcucc cuaaagcuaa gaagaacaga     2100

auuaccaaca aaaauuucgc caacaaggaa gccggcuuca ucgcucgcaa uauuguugau     2160

acaucuuaca ucgccagacu guccgcgagc uauaccgacg cuuuuauuga guucugcccg     2220

cuugccgaaa acgaggauac gacacuggga cggggcgaga aagggucuaa gaaacacauc     2280

uccgugguca auggaagccu caccgccacc augcgccauu acuggggccu gaacacacug     2340

cuugacgggg aaaauucuga gagcaaggac agauccaacc accuccacca ugcucuggau     2400

gccaucauua ucgcuuacgu gaaugacagc gucauuaagg ccuuuucuga uuucaaaaag     2460

acccaggaac aaaacaaagc gcggcugauc gccaaggaga uugaauccgc ugaguauaaa     2520

aucagccgca gauuuuucuc ccccucugga uuugagaaua acgaagccuu ccggcaggcu     2580

aucaagcgca agauucucgg ccagagccug gaguccagag aaaaugacau gacaaucaac     2640

ucugaggccc uuggggaaaa agugggcgga auuuuuguua gcaagccucc ccggaaacgc     2700

gccagaggcg cucugcacaa ggagacguuc uccucuaucg augacaaaga ucuccugaag     2760

accuacgggg gaaaggaggg cgugaaucgg gcgauuaaac uggggaaggu cagacaaauc     2820

ggaagcaaaa ucgccgacaa cggcacaaug gugcgcguug auauuuuucg gcauaaggug     2880

uccgggaaau ucuauggcgu cccuaucuac accauggacu uugcucuugg gauucucccc     2940

aacaaggccg uggugggcgg gaaggauaaa agcggaguua ucaaggacug gcugccgaug     3000

gauucuaauu augaguucug uuucucccug uuuaaagacg accucauucu gguccagaag     3060

aaagaaauga gcaaggccga acuuugcuac uucgcuggcu uugauacauc ugggggacag     3120

aucaaagugg agaagcacga caaccauuuc gccacccuga cggauaauca gagacuccug     3180

uuuaacauug cgaccccuaa ggaaguggcu ggcaaaucca ucgggaucca aaaucugaag     3240

gucuucgaga aauggcaagu gagcccccuu ggcgagguuu cuaaggccga auuuaaagag     3300

cgcgaaccua uugcccugaa guccacaccc aagcgguucc uccagggaug a              3351


<210>  3931
<211>  3354
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3931
augaagcaca uccugggccu cgaccugggg agcaacucca uuggaugggc cuucgugcag       60

caagauuuug agaauaaaca gggcaagauc auugcuaccg ggucuagaau cauucccaug      120

gaccagggaa uccuuggcga uuucgaacgg gggaacacag ucagccagac ggccgagcgc      180

accacauaca gauccaugcg gcgccugaga gaacggcauc uccugcgcag agagcggcuu      240

caccgcguuc ugcaucuccu gggauuucug ccuccgcacu augacgcgca aaucgauuuc      300

accaaaagau acggcaaguu uauugacaau gcugaaccca aaaucgccua uaacaauggc      360

aacuucauuu uuaugaauag cuucaacgag augguggagg auuuuaagaa gcaucagccu      420

caacucuucu acaaaaagag caauggggaa gagucccgga uccccuauga cuggacaauu      480

uacuaucugc gcaaaaaggc ccuuucucag aaaaucaccc agcaggaacu ggcuuggcuc      540

auucugcacu uuaaccagaa gagaggauac uaccagcuuc ggggcgagga ggaaacagag      600

aauccuaaca aggaaguggc cuuccacagc cugaaagucg uggauguuga ggcugaagcc      660

cccaauaaga aaggggagau cugguauacc auccgccucg agaacggaug gauuuacaga      720

cggacgucca agaauccucu ggacgauugg aaaggcaaga ccagagacuu uaucgugaca      780

accgaucuga acgacgacgg gacaauuaaa cucgauaagg aaggaaagga gaaacgcagc      840

uuccgggcgc cggacgaaaa cgauuggaau cugcuuaaga aaaagaccga gcaugacauc      900

gauuucucug gcaaacccgu cggcacguau auuuacgacc aucugcucca aaacccuaau      960

cagaagauca aggggaaacu ggugagaaca aucgaacgca aguauuacaa agaugagcuu     1020

aaggccauuc uggcuaaaca gaucgcccuc caacccgagc uguuuaccga aacacugcuc     1080

gcugacugca uucgggagcu guauagaaag aacgaaaccc agcagcagaa ucuucugucc     1140

aaggauuucc uccaccuguu uguugaggac aucauuuucu accaacgccc ucuuuguagc     1200

aaaaagucua ccaucgccaa cugcacacug gaaaaacggu ccuacaucga uaaggagacg     1260

aaugagagaa aagacgcgcc cauuaaggug ugugccaaga gcaacccgua uuaccaggaa     1320

uuucgcgucc uccaguggcu gcagaaucug cggaucuaug agauugaaac cgacagagag     1380

gugacagcug aguucaucga aaccccucaa gauuacgagg cccucuuuaa cuuccugaug     1440

ucucagaaag aaauugacaa ugagggacuu cugaaguauu uucucuucgc uaaaaacccc     1500

cagaucaagg auaaagccgc caaguccgaa cugaaaaagu ggcugacaac cuaccgcugg     1560

aauuaugugu acgacgcugc ggauaacagc uccaagaaau acccuaugaa ugagacgggc     1620

uaugagcuuc agcgguaccu ccaaaagguc gaagggcugc ccgccaacuu ucugacauuc     1680

gacauuaccu aucagcucug gcaucugcuu uacucuguua aagauaaggu ggaguuugaa     1740

aaagcucuga agaaguucgc caaaaagaaa cagcucgaca agaccagcuu cguggauucc     1800

uuuaaaaacu ucaagcccua uccuaaugag uacggaucuu uuucugaaaa ggccaucaaa     1860

aagcugcugc cgcuuaugag auucggcaac cacugggacu uuaaucggau cgaugucucc     1920

acacagaaac gcauugacga ucucaucaac gggguggcua augaggacau uagaaccauc     1980

auucgggaga aggccgaaaa auaucaacug aacagcgaga caugcuucca ggaccugccc     2040

cucuggcugg cgcaguacgu uguguacaau agacacgcug aagccacgga uaucgagaag     2100

uggaccucug ucagcgaccu ugaagccuau cuggaggauu uuaagcaaca cucccuccgc     2160

aacccuaucg uggagcaggu ugugacagaa acccugcggg ucgugagaga cauuuggcag     2220

caguacucug gaggccaagc uaauuucuuu gaugagaucc auauugaacu gggccgcgag     2280

cuuaaaaaga cagccgagga aagaaaacag cucagcgaca agaaccagga gaaugaaaac     2340

accaaucugc ggaucaaaaa gcugcucgcu gagcugaaag aagaugccuc cauucagaac     2400

guccgccccu auagccuugu gcaacaggag cugcucaaga ucuacgagga agggguuuuc     2460

ucuuccacgg acgagaucga agaggauauu cagaagauca gaaagaaggu ggaaccuaca     2520

cccccggaga uucagcggua uaaacugugg cuggagcaaa aguacaaaag cccuuauacc     2580

ggagaaguca ucucucuuac caagcucuuu acagaggaau acgagauuga acacaucauc     2640

ccccaguccc gcuacuauga cgauagccug uccaauaagg ugauuuguga gucugcgguu     2700

aacaaacuga agaccaacca gcucggccug gaguucaucc aaaaucauca cggggaaaaa     2760

gugagaauua cggccaacaa ggaggucaca aucuuuaccg aggaacagua caaagaguuc     2820

gugaagaagc acuaugcuaa uaacagcgcc aaacagcaga aacuucugcu caaugaaauu     2880

ccugagaaaa ugguggcucg gcaacugaac gacacacgcu acaucuccaa gugcauuucu     2940

gaaauccuga gcaaaaucgu uagauccgau gagaaggacg aaggagucaa uagcaagaac     3000

auuauccccu guaccggcaa aauuaccaca gcccuuaagc aggacugggg gcugaaugau     3060

guguggaacg agcucauccu gccucgguuc gagcgcauga aucagcucac gcagacccaa     3120

cuguuuacau cuuauaacga cagacuucag aaaaaucugc ccaccgugcc gauugaauac     3180

ucccagggcu uccagaagaa acggaucgau cauagacacc augcgaugga cgcccucguc     3240

aucgcuugcg ccacacgcga gcacauuaac uauaugaaua accaacacgc ucuggauaag     3300

agcaaggaca aaaaggaaaa acagcagucu cggaucgauc ugcauccucu uuga           3354


<210>  3932
<211>  3354
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3932
augcagacca agaaccugcu cuacacacug ggccuugaca ucgggauugc cagcguggga       60

uggugcgucc ugggcgagaa uagaaucauu gaucucgggg uucgguguuu cgacaaagcu      120

gaaacgcccg aucgcggaga gucccugaac aaggugagac gggacgcccg ccuuaugaga      180

cggcgccuga gacggcgcgc guggagacuc accaaacugg cucggcugcu caagcacgcc      240

ggccugaucg auucuacacg cauugugaga gaccggcaug auggggccgu cagcccuugg      300

caacuucgcg uggaaggacu ggacagaaaa cucgcuccgg aggaaugggc ccggguuauc      360

uaucacaucg ugaagcauag aggcuuucac uggauuuccc gcgcugagga gcggaaggcc      420

gaaucugaua aaggcgaagg gggaaagguc aaacagggcc ugagcgcgac caagcagcuu      480

augguggaaa agaauuacag auccgccgcu gagaugguuc ugacagaguu ccccggggcc      540

cagcgcaaca agcggggaga auauaccaag gcucucucua gaauccugcu ggccgcggag      600

cucacaaccc uguuugaacg ccaacaggag cuuggcaauc cucaugccgg gcccgaccug      660

cagacgaaaa uucucggaaa cggcgauaga aagagcggcc ugcuuuggau gcagaaaccu      720

ccccuguccg ggagcgcucu ccugaagaug cugggaaaau gcaccuucga acgggaggag      780

uaccgcgccc cuaaggcuuc uuuuacagcc gaacggcacg uguggcucac caaacugaau      840

aaccuuagag uccuggugga cggcgugaca cgcccgcucu ccgaucccga gcggagacug      900

gcccuuagcc ugccuuauca acaggcuucu gacuucaccu acaagcagcu ccgggcggcc      960

cuguguaaga augggcugcu ccccgacucc uuucaguuca gcggacuggc uuauccuacg     1020

ggcgcccaac agcccgaugg gaaagccaag aacccggaaa augaccgccu ugucaaacug     1080

ccugcuuggc aggaguuuag aaagacacuc cgggaugccg gacuggaaac cgagugggag     1140

ucuaucgcgg gcauggcugc cggcgggcaa cccgaccugc uugaucagau uggauggguu     1200

cucuccgugu ucaaagacgg cgaugaagug cugagagagc ugcgcaagau cccucucccc     1260

gggggagaaa agauggucca ggcccugagc ggcguguccu uugacacauu ccacaaccuu     1320

ucucugaaag cucuccggaa gauuguuccc cauauggagg ccgggcugag auacgaugag     1380

gcuugccugg ccgcgggcua ccaccauagc gaccuucgca aagauaccga cgagagaucc     1440

cucuaucugc cuccgcugua cagcggacgg accacagacg gccgcauggu guuuagagau     1500

gacaucgaug ucccccggaa uccugugguu cuccgcgcca ucaaccaggc uagaacggug     1560

gucaauucug ugguccggcg cuaugggucc cccagcucua ugcacauuga gauggccaga     1620

gaccugagcc ggccuuggga ugaacgcaac aaggugaaaa gagcucaaga ggaauaccgg     1680

gacagaaacg agaaacuuaa gaucgauuuc gccgcggagu auggaauuga acccaaaucc     1740

cgcgaguucg aaaaguuucu gcucuaccgg gagcagggcg ggaaaugugc cuauucucug     1800

aagccgcugg accuugauag aguucuccgc gaccugggau acgcugaagu ggaucacgcc     1860

cugccuuaca gccgguccuu cgacgauagc aaaaauaaca agguccucgu gcuggcugag     1920

gagaauagaa acaagggcaa ucgcaccccc uaugaauacc uugacgggac aucugagucc     1980

ccucggugga gagaauuuaa agccuucguu gagaccaaca aggccuaucg ccuggcuaaa     2040

cgggcgagac uccugcggaa ggacuuuagc ggcaaaggau cugaagaguu ccgcgauaga     2100

aaucugaacg acacacggua caucucccgc uuuuucaaga auuaugugga gagauuucuu     2160

aaacucgccg aagagagcga ggaacggacc ugcgucgugc uguccggcca gcugacggcu     2220

uuccucagag cccgcugggg gcuggccaag gugcggggag auucugacag acaucacgcu     2280

cuugaugccg cgguuauugc ugccuguagc cgcuccaugg ucaagcggcu gucugacuac     2340

gccagacgcc augaacucga gaaagugcgg acaggcuuug uggauuucga gaccggggaa     2400

aucgucgacg cuggaaagua uagacagauc gagcgcgauu uucccagccc uuggcccuac     2460

uucagagacg aacugcugac ccggcuuaac acagaugacc cggccgagcu gcgcgagaga     2520

augcaauccc ucggcagcua cccucccgaa gcgcuggaga cccuccggcc cauguucgug     2580

ucucgcgcuc cucagagacg gaaugggggc gccgcccaca aagauacaau uuauagacag     2640

acggaacgcc ugaagaaagu uggauccgug acccagaagg ucccgcuugc ugcccugaac     2700

cuccgggacc uggagaaucu gguggacccc cauagaaacg aaaaacuuua cgcugccauc     2760

cgcgcgcggc uggaggccca cggcgggaag ggagagaagg cuuuuccucc caauaacccu     2820

cucagaaaac ccgaucggga cggcaacccu acagggccca uuguucgcac cgugacacug     2880

gucaucgaua agaugagcgg cauuccggug agaggagggg uugccaaaaa ugacaccaug     2940

cuccgggugg auaucuuccg cucuaagaag gacggcaaau uucaccuggu cccucuuuau     3000

gugcaucacg cuguggccaa gcaacugccc acgagagccg ucaucgcuua caaagaugaa     3060

ggagagugga ccccuauuaa caaucaugac uucgauuuuc ucuucgcgcu guaucccaac     3120

gaccugguuc ggaucacacu uaagaaagaa cgccacuuug gcuacuaugc cgggugcaau     3180

agagcuaccg gagccguguc cauuugggcc cacgaucgga accagcgcau cggcaaggac     3240

ggguucauug acggaaucgg cgugaagaca gcucugagcg ucgagaaauu ucauguggau     3300

acccucggca auguuuaccc ggccccuccc gaaacgagac gggggcuggc guga           3354


<210>  3933
<211>  3357
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3933
augggcacca gauacagcuu cgacaucggg acaaacucca uuggaugggc cguguggcgg       60

acgggccgcg aucccaaggg gaucuuugga gagaaagacg cuccucugga acuccugggc      120

gccgggguca gacuuuucaa ggauggacgg aauccgaaag acggccaguc ucuggcgacc      180

augcgccgga uucccagaca agcucgcaag agacgggauc gcuuuguucu cagacgggag      240

gcccuggugg ccgcucuuau cgaagccggc cugaugccug cuagcgagaa agaacgcaag      300

gcccucgcgg accuggaucc cuaucagcug agagccgcug ggcucgacaa ggcccuggcu      360

ccucacgagc uuggacgggc cauuuuccau cugcaccagc gcagaggcuu uaaauccaac      420

cggaagacag auagaaaauc ugacgaucgc gacaagggga aaaucgcgac cgccagcgcu      480

gcccucgcua agaaguucga ggaugaaaau gcccggacau uuggagaguu ccuguggaga      540

cgccaucggg accccgacau gaaaagacag gccgugcuua aggcucacga uccugcgaaa      600

ggcaagccgg accugcgcgc cgcuaaacuc cggcaaccca ccagaauccg ccuggaaggg      660

cagggagcca cagcccugua cgaguuuuau ccuacccggg agaugcucug ccaugaguuc      720

gaugcucugu gggaagccca ggcgagacac caccccaugc uucugacgcg cgaagcuggc      780

gacaagauuc gguccauccu cuuuagacag cgggagcuga aaccuccccu uauuggccug      840

uguaccuucg aauacgccgc cggggagaag gagucucgcc ucccgaaggc ucugccuagc      900

guccaagcca gagaaaucua ugagcggcug ggacauauua gacucuccac aggcagcggg      960

accgaucgcc cccugaaauc uuccgaacgg gacgaucuug cuacagcccu gcuccacugc     1020

ggacugacca agaucaaagu gagcaagacg aaaggccuug cguuuaagac agaguucaga     1080

aaggcccugc gccucggggg agaaguuaga auuaacuucg aggagauggg cgaagagggc     1140

cugguggacc ugcggaccaa ucgccuccug guccaggaug accauuacgg gccuagaugg     1200

cgggcucuuu cuugguccgg aaaagaugcc uuugugaaga aacugcucga agcuacagac     1260

gacgauaagc ugguugcccg ccugaugacc gaggacggcc uuagcgcggu gcaggccaga     1320

aacgcugccu cuaucccccu ccccgauggg uauucccggc ugggaccuac cgcuaaugcc     1380

gccauccugc aggcucucau ugacagccgc gaugaaaacg gcuucgucgu gacauacgac     1440

aaagcgguga gacgggccgg ggagcgccug ggcaaggauu ggcaccacuc cgacgagaga     1500

gauggagaaa uccuugacgc ucugccguac uauggcaagg uccuccaacg gcauauucug     1560

cccgggauga uggauccugc cgacagaaaa gaugaggcca aguuuuacgg acgcaucaug     1620

aaucccucug uucacauugg ccugaaccag cuucggagac ucgugaaugc ucugaucgaa     1680

cgcuucgggc cucccgacca gaucgugguc gagcuggccc gggaccucaa aaugagcgcg     1740

agagagaagg aacaagagca gcgccggaac agagauaauc gcgaagcuaa cgagaaacgg     1800

acgaagaagc ugguggaacu uggacaggac aauaacggcg agaacagagc ccggcugaaa     1860

cucuaugaug agcagcaacg cgccgggaau ggcguugcuc auuguccguu uacccuggac     1920

ccuauuggaa ucacacagcu guucuccccc gaaauugaga ucgaacacgu gcuuccuaga     1980

agccggaccc ucgaugacuc ugccgcgaac aagguccugu gcuuucgcac aauuaauaga     2040

aucaaacggg gcaagacccc cugggaggcu uuccaugccg auccuaaaug gcccgacauc     2100

gccgcuaacg ccgaaaagcu gccgccuaau aaaagauggc gcuuucggga ggaugcuaug     2160

gacguguucg aggccagaac guccgggaag auggauaaga caacccucga acagauggga     2220

cugaccacag gcuuucaggc gcgccaccuu aacgagacca aacacaugag ccggcuggcc     2280

aaggcuuacc ucgggaaaau uugugacccc gaugccguuu augugacacc cggagcucug     2340

acgucucugc uuagaggcaa gugggggcuc aauagcaucc uguccgacga caaccugaaa     2400

gaucgcaccg accaucggca ccaugccauu gaugccauca caauuggcgc uaugaccaga     2460

ucucuccugc uugaacugaa uacacucgcg ggaaaggccg agcaaagcga cuacgaugac     2520

gucgugggca aggucccuug gccguucgau aacuuucgcg acgcugugag auccguugug     2580

gaucgggucg ugguuagcaa uaaacccgaa cacgggaagc agggagcccu gcacgaggac     2640

accgccuaug gcaucguggg ggaugaggcu gaagccgagg aaauuggaaa ccugguccgc     2700

agaaaacuuc ugguggaccu cacgccuggc gagguggacc ggauccgcga ugcggggcug     2760

agaaaggcua ugcaggccgc cguugcuccc ucucgggacg ccagaggcaa agucuccgau     2820

gcgaagggau ucgcugccgc ccucgacacc uucgcuaagg cccagggcau gcgccggauc     2880

agagugggga aagaggauau gagcgcugug acaauucaug accgccaaac cgauaaggcc     2940

uacaaagcgg ucgccccugg agaaaaucac cauguggacg uugugcagau gcgggauggc     3000

gcuuggaagg gguuugccgu cacaguguuc gaggcuaacc agaaagacuu uagaccccag     3060

ugggaacggg agaagcuggg aggcaaacuu guuaugcgcc ugcacaaggg cgaugccgug     3120

gaacucgagu cuuccggggu cagacuggug aaguucguuc aacagaucug gaugaaaagc     3180

aaucuggugc uucugaccga gcauaacgaa ggaggcgacc uccagaagcg gcaccaagau     3240

gccgacgacc cuuuucgcug ggauuucgcu aacauuggga aacugaagga gagaggaugc     3300

gucgcgguga aaguggacga acucggccgg gucacgaagc gcaucuccaa uacauga        3357


<210>  3934
<211>  3360
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3934
augggcuaca gaaucgggcu ggacguggga auuaccagca caggcuaugc cguccucaag       60

acggauaaaa acgggcugcc cuacaagauc cuuacccugg acuccguuau uuauccucgg      120

gcugagaauc cgcagacagg agccucucuc gcggaacccc gcagaaucaa acggggccug      180

cgcagacgga cccgcagaac aaaguuccgg aaacaacgca cccagcagcu uuuuauucac      240

agcgggcugc ucuccaagcc ugagaucgaa cagauccugg cuacacccca agccaaguac      300

ucuguguaug agcugagagu ggccggacuc gaucggcgcc ugaccaacag cgaacuuuuc      360

agaguccugu acuuuuucau uggccaucgg ggcuuuaaau ccaaucgcaa ggcugagcuc      420

aacccugaga augaagccga caaaaagcag auggggcagc ugcuuaacuc uaucgaggaa      480

auuagaaaag cuaucgccga gaagggauau cggacggugg gcgagcugua ccucaaggau      540

cccaaauaca augaccacaa gagaaacaaa ggguauauug auggauaccu gagcaccccu      600

aaucgccaga ugcugguuga cgaaaucaag caaauucucg auaaacagcg ggagcugggc      660

aacgaaaaac uuacagacga guucuaugcg accuaccugc ucggggauga aaauagagcc      720

ggaaucuuuc aggcucagcg cgacuucgau gagggcccgg gcgccgggcc cuaugcugga      780

gaccaaauca aaaagauggu gggcaaggac auuuuugagc cuacagaaga ucgggccgcg      840

aaagccaccu acacguucca guauuuuaac cugcuucaga agaugacauc ccugaauuac      900

caaaacacca caggggacac cuggcauacc cucaauggac uggauagaca ggcuaucauu      960

gacgccgucu ucgcuaaagc cgagaagccc acaaaaacgu acaagccuac cgauuuuggc     1020

gaacugcgca agcuccugaa acuucccgac gaugcccggu ucaaccuggu gaacuauggg     1080

agccuccaga cacagaagga gaucgaaacc guugagaaaa agacaagauu cguggacuuu     1140

aaggcuuacc acgaucuggu caaggugcuu ccggaggaaa uguggcaauc ucgccagcug     1200

cucgaccaua uuggaaccgc gcugacgcug uauuccagcg auaagcggag acgccgguac     1260

uucgccgagg aacucaaucu gccugcugag cuuaucgaaa aacugcuccc ccugaacuuu     1320

ucuaaguucg gccaccuguc caucaaaagc augcagaaua uuaucccuua ucuugagaug     1380

ggccaggugu acucugaggc cacaaccaac accggguaug acuuuagaaa gaaacaaauu     1440

uccaaggaca caauccggga agagauuacc aaucccgucg uucgcagagc cgugacaaag     1500

acgaucaaaa ucguggaaca gauuauccgg agauacggaa agcccgaugg cauuaacauc     1560

gagcucgcuc gcgagcuggg gcggaauuuc aaagaaagag gagacauuca gaagcgccag     1620

gauaaaaaca gacaaaccaa ugacaagauc gccgcggagc ugacagaacu cggcauuccu     1680

gucaacgggc agaauaucau ccgguacaaa cugcacaagg agcagaacgg cguggauccg     1740

uauaccggag accagauucc cuuugaacgc gcuuucagcg agggcuacga gguugaucau     1800

aucauuccuu auuccaucuc uugggacgau agcuacacaa auaaggugcu uaccuccgcc     1860

aaaugcaaca gagaaaaggg gaaucggauu cccauggucu aucuggccaa caacgagcaa     1920

cgccucaaug cucugacgaa caucgccgac aauaucauua gaaacagccg gaaacgccag     1980

aagcugcuua aacagaagcu cucugaugag gagcugaagg acuggaaaca aagaaauauc     2040

aacgauaccc gguuuauuac acgcgugcug uacaauuauu ucagacaggc uaucgaauuu     2100

aacccugagc ucgagaagaa acagcggguu cugccccuua auggagaagu gaccuccaag     2160

auuagaagcc gcuggggcuu ccugaaaguc cgggaggacg gggaccucca ccaugccauc     2220

gaugcgacag ugaucgccgc uauuaccccg aaguuuaucc agcaagucac gaaguacucu     2280

cagcaccagg aagugaaaaa caaucaggcc cuguggcacg acgcugagau uaaggaugcc     2340

gaauacgcgg ccgaggcuca aagaauggac gccgaucugu ucaacaaaau cuucaaugga     2400

uuuccucuuc ccuggccuga guuccucgac gaacugcugg cucgcauuag cgauaacccc     2460

guugagauga ugaagucccg gucuuggaau acauauaccc cuaucgaaau cgccaaacuc     2520

aagcccgugu uugucgugag acuggccaac cauaagauua gcggcccggc ucaccuugac     2580

acaauccgcu ccgcgaaacu guucgaugag aaggggauug uucucagccg ggugucuauc     2640

accaaacuga agauuaacaa aaagggccag gucgccaccg gagacggcau cuacgauccu     2700

gaaaauucca acaaugggga caaaguggug uauagcgcua uuagacaggc ccuggaagcc     2760

cauaacggau cuggcgagcu ugcuuuuccc gauggguacc ucgaauaugu ugaccacgga     2820

acaaagaagc ugguccgcaa agugcgggug gccaagaaag ucucccugcc cgugagacuc     2880

aagaauaaag cggcugccga caacggcagc augguucgga ucgauguguu caauacgggg     2940

aagaaguuug ucuucgugcc uaucuacauu aaagacaccg uugaacaggu gcugccgaac     3000

aaggccaucg cucgcggcaa aucccuuugg uaucaaauua cagagucuga ucaguuuugu     3060

uucagccugu accccggaga caugguccau aucgagucca agaccggcau uaaaccuaag     3120

uacucuaaua aggagaacaa uacaagcgug guucccauca aaaacuuuua uggguacuuc     3180

gauggagccg acaucgcgac cgcuuccauu cucgugagag cccacgauag cucuuauacg     3240

gcccggucca ucggcauugc ugggcugcug aaguuugaaa aauaccaggu cgacuauuuc     3300

ggacgcuacc acaaggugca ugagaaaaag agacaacuuu ucgugaagcg ggaugaauga     3360


<210>  3935
<211>  3360
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3935
augaacgaca agaaaaaucu gggcccccuc accuucgggu uugauaucgg aauugccagc       60

gugggcuggg cuguccuguc cccuacaaga aucguugacc uuggggugcg gugcuucgau      120

aaggccgaga cggcgaaaga aggagagucu cugaaccucg cucgcagaag cgcccggcug      180

augcgcagac ggcuucgcag acgggccugg cgccugauua agcucgcuag acugcugaaa      240

cacgaaggcc ucaucgccga caccaagaca uuuauucagc cguccgcuuc ucaugccagc      300

cccuggcaac ugcggaagga ugggcuugac cgcgcgcugu ccccugagga augggccaga      360

gugcucuacc accuguguaa acaucgggga uuccacuggg ucucucgcgc ugaggccaga      420

aaggcugaug gcgacaccaa aggcgaaggg ggaaagguga aacagggccu ugccagcaca      480

accaagcuga ugaaugauaa guccuaucgg acagcggccg aaaugguucu cgcugaguuu      540

cccagcgccc agagaaacaa acgcggggaa uacaccaagg cucugucucg ggagcugcuc      600

gacaaugagc ugaaacagcu uuucgaaaga caacgcgagu auggaaaccc ucaugccgau      660

acgaaucugg aagccaagau ccggggcucc ggggaccaca aaagcggacu cuuuugggag      720

cagaagcccg cucugucugg caacgaucuu cugaaaaugc ucggcaagug caccuucgaa      780

aagcaggagu acagagcgcc uaaagccucc uuuacagcug agcgccacgu guggcugacc      840

cggcugaaua accucagaau cauugucgac gggcgcacac ggccgcuggc cgacgccgaa      900

agaaccaugg cucuuagccu gcccuaugaa caggugggag auuucacgua caagcaacuc      960

cgcgccgcgc ugguuaaagc uggccuuaug ccugacucuu uuaaguucgc cucccuggcc     1020

uaucccagcg aguuucagaa agcugagggg aaggccaagg auccugaauc cgcugcccuc     1080

acaaaacugg cggccuggca ggagcugcgg gcuacccuca agaaagccga ccuggagucu     1140

gaauggcagc aaaucagcug ugcugcccuu gagggacagc ccgaaacacu ggaugagauu     1200

gcgggcgugc ucuccgucuu caaggacgau gacgaagugc ugaaugagcu gcagaaacuu     1260

gggcucccgg gaggcgccaa gaccgagaac gcucugcugg auaucagauu cgacaaguuu     1320

caugcccuca gccugaaagc ccuucggaag auugugccuu acauggaacg cggccugaga     1380

uacgaugagg cuugcgaacg ggccgaguau caccauucuc aacuccacaa agucggggag     1440

ggaaauagaa aguaccugcc cccucuguau aaaucccgcg acaaggacgg ccggaugguu     1500

uucaacgaug aacuugggga cauccccaga aaucccgugg ugcuccgcag ccugaaccag     1560

gcuagaaagg uccugaacgc gcucauugcc cgguacggau cuccuagcuc cgugcacauc     1620

gagauggcuc gcgaacuguc uaaaccguuu gaugagagaa ugaagguugg caaagaacag     1680

gaggccuauc gggagcgcaa ugaaaaggac aaacagaccu ucguggccga guuugggauc     1740

gucggcgcug ccaagggagg cgaguucgag aaauggaaac uuuacagaga acaacagggg     1800

aagugugcgu auagcauuca gggaaucgau cugaaccggc uccuggagcc cggcuacgug     1860

gagauugacc augcucugcc uuacucccgc agcuuugaug acucuaaguc caauaagguu     1920

cuugugcucg ccgccgaaaa cagaaauaaa gggaaccgga cacccuauga guaccuggau     1980

ggagaacaca auagcgagcg cuggagacag uucgucggcu uuguggaauc uaacaagcug     2040

uaucggcaag cuaaaagauc ccgccuccug cggaaggacu ucgggaagga gcaggccgau     2100

gaguuuagag aacgcaaucu uaacgacacg cgguacgucu gcagauucuu uaaaaaucag     2160

guggagcagu uccugaagcu cgcggaaggc agcgagucca aacgcugugu ugugcugucu     2220

ggacaacuga ccgcuuuccu ucgggccaga uggggccuca ucaagguccg cgaugagagc     2280

gaccggcauc acgcccugga cgcugugguu guggccgcuu gcacacaugg gauggucaaa     2340

agaauguccg auuaugcccg gcgcaaggaa cuggaccagg uggcggaggg auuuguggau     2400

guugaaaccg gcgagauuau caacgccgcu auguuccagc agcuccacga acacuuuccu     2460

gaccccuggc cgcauuucag agaugagguc caaaugcggc ugaagaucga cgauccugcc     2520

cagcuucgcu cugaccugcu cagacugggg aauuacgcug aggaagaugu gcacgccgug     2580

cggccccugu uugucagcag agccccucag cgccggaacu ccggagcugc gcauaaagag     2640

acaauuuaug gccaacccga aagacuuaag gcccaagggg gcgugaccga gaaaguugcu     2700

cugacggccc ucacaaugaa ggaccuggau cgccucaucg acccucaccg gaaugaaaaa     2760

cuguacgccg cuauuagaaa gcgccuugag gaguucggag gcaaggccga uaaagcguuu     2820

gcugccagca acccccugca caagccggac aaagaaggga acccuaccgg acccaucgug     2880

cggaccguca cacucgagau ugacaagcug ucuggcgugc ccguuagagg gggaaucgcc     2940

aaaaaugaua ccaugcugcg cguggacguc uucacaaagg cuggcaaauu ucaucuugug     3000

ccuguuuacg ugcaccauaa ggucacgggg cugccgaaca gagccaucgu ggcguccaag     3060

gaugaagacg aguggaccgc uauugaugac agcuucgaau uucucuucuc ucuguauccc     3120

aaugaucucg ugacaaucac ccagaaaggc aaggccccug uccggggaua cuaugccucc     3180

ugucaccgcg gcacaggggc uuuuagccug ugggcccaug acagagcuuc uagcguugga     3240

aaaggcgggc agauugaggg aaucggcauu aagaccgccc uugcggugca gaaauacaac     3300

guggaugucc ugggcacgac cuaugccgcu ccccucgagc agcggcgcgg gcuggccuga     3360


<210>  3936
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3936
augagaaagg ugaccaucgg cuucgacauu ggggucagcu ccguuggaug gucuguggug       60

gauaugaaca auggcgccau cgucgagaca ggggugcggc uguuuagcgc uuccucugcc      120

gaaaacaaug uugagcgcag aaacaaucgg cagacgcgca gacucaaccg gcgcagaaag      180

aaucggcuga aggacgugaa auaccuucug gaaaccaacc acuucacauu ugaucaagag      240

cagggaacca aucccuauga acuccgcguc gcgggccugc auagaaagcu cacacggagc      300

gagcuggcuc gcgcccucua ccaccuggug aaaagacggg ggauuuccua ugaccugacc      360

gauaucgagg aagaggaaau uguuacaacc ucugccuaca agacggcucu cggaaagaac      420

cagagccagc uggccgagaa aaccccuggc gagauccaau ucgcucgccu ugaaaauuau      480

ggcaagguga gaggguccau cgagauuaac gaacaggaca uccugcucaa uauuuuuccg      540

acaagcgccu accugaacga ggcgaaacgg auccuugcca cccagagaaa guucuauccc      600

gaaauuacag aggcuuuuau cgagaaauac ugcaccauuc ugacgcgcaa gcgggaauac      660

uaugucggac cuggcacaga gaagaauaga accgauuacg ggaucuaucg cacaaacgga      720

gaaacccucg acaaccuguu cgagauccug auuggcaaag auaaguuuua ccccaaugaa      780

gaacgggccg cugggaacuc uuauaccgcc cagcuuuuca auuuugugaa cgaccugaau      840

aaccuuaaaa uccaaacaau ugaggaucag aagcugacgc cuaaacagaa ggaagagauc      900

auugaacagg ugaaaaccgc ggagaagaag gucucccuca uccaacugau cagcaaaauu      960

acaaagaccc ccaaagacca gaucacagga uacagaaccg auaagaaugg caaaccugac     1020

cuucauucua uggccguuuu ccgcaaggug cggaagcagu uucugacgau ggaaauugac     1080

auccuccacu ggccggauga gcugcugaac gaccucggca gacugcuuac acugaauacc     1140

gagaaagggg aaauucgcaa gcaacucacc aaagauuuca ugaagcggua ucccauccug     1200

aacgagcagc uuguggacca gaucauugaa aauagacaua ucuucgcugu cuccggaaac     1260

cacaaauggc accgcuuuag ccugcggaca augaaugagc uccugccuga gaugcuggcc     1320

accucuaagg aacagaugac acuccugucc gagagaggca ugauuaagca acauaaacgg     1380

gauuaccuua agaugaacga acuggacgcu cgccucauca gcgaggaaau uuacaauccc     1440

gugguuggga aauccgugag agaggccauc aagcuguuca acgagcugau gaaaaaguau     1500

caggaagucc gguacguggu ucuugagaug ccuagagaug acaacgaaga gaaggccaaa     1560

aaggaacucc agaaauauca gaaggagaau gagaacgaaa aagagauugc ucugucugaa     1620

uuucaacgca agggauucuu uagcgaugcg cagcuggagc gggcccucau caaagacaga     1680

aagcugaaga cgaaaauccg cuucuggcac cagcaggaag gcaagugucu uuacuccggg     1740

caaauuauca cccccgugga gcugcucaca aauagcuuuu cuuucgauau ugaccauauc     1800

auuccgcagu ccgucagcuu ugacgauucu cugaacaaua aagugcugug caccaaggag     1860

augaacgacc agaaaggaaa gcagacaccu uauggcuucc uucucaaugg caacgggcaa     1920

ggauuugaua gauucaaggc uauggucaau gccaaccggc ugaugagcaa aggcaagaaa     1980

aagaaucugc ucuuuaugga agaccugaac aauaucgaga cccgcaaaag auucaucgcc     2040

cggaaccuug uggauacgcg cuacgcuucc agaguugugc ugaaugaaau ucagcaguau     2100

cuccgggcca agaacaagga cacccaaguc gcggugguuc gcgggaaauu cacagcuacc     2160

cugagaaagc acuggaacau caauaaaucu cgggagacac accaucacca ugcccuggau     2220

gccagcauua ucgcuauuac gccccuucuc uccgugugga agaaaaagca gacccugauc     2280

ccuaagacac ugagcgagga aaaacucgag uuugaauacg acgauaucgu cgaggacacc     2340

auuuucgaaa aggcccugua ccagcuuccc aaagagaacu uuaaggagca gcugaauaac     2400

ucucaaccca auaucaaauu cuccuaucag guggauaaga aaaugaaccg caaggugagc     2460

gacgcuacaa uuuacucugu uaagcaggcc aaacucucca gcgauaagca guccgaagag     2520

uauguccugg cgaaaaucaa ggacauuuac ucuguggaag gauauaaaac cuuugccaag     2580

aucuacgaga aggacaaaag caaguuccug auggcucaaa augauccuaa aaccuuugaa     2640

aaacuugagg agaucauuca guccuauccg ucuacaagag aaaucauugu gggcgagaaa     2700

gucaaggugg uugacgugag ccccuucgaa cucuaccgga aggaguacgg guauaucacg     2760

aaauacgcca agaaaaacaa uggcccuauu aucagacagc ugaaguauua cgaucacaaa     2820

auuggaaugc aucuggacau caccaaccag aagaauaacg gcaaggggaa aaaggucauc     2880

cuccaacagc ugaaauccug gcgcacagau guguauuaca aucacgaaaa gcaggaguau     2940

gagauuaugg gacuuaaaua cagcgaccug cgguuuaaga aggaucaaua cggcaucccc     3000

aaagaaagau aucaggaggu uaagcagaug gagaaaauug cuccucaguc ugaguucugu     3060

uuuucccucu accgcaacga ccggaucaag gugcugaaua aagagaccga ugaagagguc     3120

gaacugcuuu ucggggccag aaacuauaca agcgcgggau acguggaccu gaagcccauu     3180

gaucgcguug aguuuggcaa aaaggaaugg aucccggugu aucaauacgc caccucugac     3240

gggaagugca aaaagaaauu cgcuaagaaa ggcuaugaga ucuacaaggu caacacgaca     3300

auucucggag aguccuacua uaucaagaaa gaacggaugc ugccuaagaa uauucucgau     3360

uga                                                                   3363


<210>  3937
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3937
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggugcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcagag uccggcugaa ucgccuuuuu gaggaaaucg ggcugaucac cgacuucaca      240

aagauuagca ucaaccucaa uccuuaccag cugagaguga aaggacugac cgaugagcuc      300

uccaacgaag aacuguuuau ugcgcuuaag aauaugguua agcaccgggg caucucuuau      360

cuggacgaug cuagcgacga uggcaacucc ucuguggggg acuacgccca gauugucaaa      420

gagaauagca agcagcucga aacaaaaacc ccgggacaaa uccagcugga gcgcuaucag      480

acauacggcc agcuuagagg ggauuucacc guggaaaagg acggaaagaa gcaucggcug      540

auuaacguuu uucccacguc cgccuaucgc agcgaggcuc ucagaauccu gcaaacccag      600

caggaguuca auccucagau cacagaugaa uuuauuaacc gguaccugga gauccucacc      660

ggcaagagaa aauauuacca cgggcccgga aaugaaaagu cucgcacaga cuacggccgg      720

uauagaaccu ccggcgagac gcuggauaac auuuucggga uccuuauugg aaaaugcaca      780

uuuuacccug acgaguuccg cgccgcuaag gccagcuaua ccgcgcaaga guuuaaccug      840

cucaaugacc ugaacaaucu uacagugccc accgagacca aaaagcuguc uaaagaacag      900

aagaaccaga ucaucaauua cgucaagaac gagaaagcca ugggcccugc uaaacucuuc      960

aaauauauug ccaagcugcu guccugugau guggcugaca ucaaagggua ccggauugau     1020

aagagcggaa aggccgaaau ccauacauuu gaggcguaua gaaaaaugaa gacgcucgaa     1080

acccuggaca uugagcaaau ggaucgcgag acacuugaca aacuggccua cgugcucacc     1140

cugaauacag aacgggaggg cauccaggaa gcucuugagc acgaguucgc cgaugggucu     1200

uucucccaga agcaggucga cgaacugguu caauuuagaa aagcuaacag cuccaucuuc     1260

ggaaagggcu ggcacaauuu uucugugaag cucaugaugg agcugauucc ggaacuguac     1320

gagaccagcg aggaacagau gacgauccuc acacgccugg gcaaacagaa gaccaccucc     1380

agcucuaaca aaacaaagua uauugaugag aaacuucuga ccgaagagau cuacaauccc     1440

guggucgcca aguccgugcg gcaggccauu aagaucguua acgcugcgau uaaagaauau     1500

ggggacuucg auaauaucgu gaucgagaug gccagagaaa caaacgagga cgacgagaag     1560

aaagcuauuc aaaagaucca gaaagccaau aaggaugaaa aagacgccgc uaugcucaag     1620

gccgcgaacc aguacaacgg aaaggcugag cugccucaua gcgucuuuca cggccauaaa     1680

cagcuggcca cgaagauucg ccuuuggcac caacaggggg aacggugccu cuauaccgga     1740

aaaacaaucu cuauucacga ucugaucaau aaccccaauc aguucgaggu ggaccauauc     1800

cugccucuca gcauuaccuu ugaugacucc cuggccaaca agguucuugu guacgcuaca     1860

gccaaucagg aaaaaggcca aagaaccccc uaucaggcuc uggauucuau ggacgaugcc     1920

uggagcuucc gggagcucaa ggcguuuguc cgcgagucca agacgcugag caacaaaaag     1980

aaagaauacc ugcuuaccga ggaagacauc ucuaaguucg augugcggaa aaaguuuauu     2040

gagagaaauc ucgucgacac aagauacgcc ucccgcgugg uucugaacgc ucugcaggaa     2100

cacuuccggg cccauaagau cgauaccaaa gugagcgucg ugagagggca auuuacaucu     2160

cagcuccgca gacacugggg cauugagaag acccgggaca cguaucauca ccacgcuguu     2220

gacgcccuga ucaucgcggc cuccagccag cuuaaucugu ggaaaaagca gaaaaacaca     2280

cucguguccu acucugagga ucaacugcug gacauugaaa ccggagagcu uaucagcgau     2340

gacgaauaua aggaguccgu cuucaaggcu ccguaccagc auuucgugga uacacucaaa     2400

ucuaaggagu uugaagacag cauucuguuc uccuaucagg uggauagcaa auuuaaucgc     2460

aagaucucug acgccaccau uuacgcuacc agacaggcca aaguuggcaa ggauaaagcc     2520

gacgagacau auguccuggg gaagaucaag gauaucuaca cgcaagacgg auacgaugcu     2580

uucaugaaaa uuuauaagaa agacaagucc aaauuucuca uguaccggca cgacccucag     2640

accuucgaaa aggugaucga gcccauucug gaaaacuauc cuaauaagca gaugaacgag     2700

aaaggcaagg aggugcccug uaaucccuuu cuuaaauaca aggaggaaca uggguauauc     2760

cgcaaauaca gcaagaaggg aaacggcccu gaaauuaaau cucugaagua uuacgauucc     2820

aaacucggga accacaucga cauuacaccg aaggauagca auaacaaagu cgugcugcag     2880

ucucugaagc ccuggagaac cgacguuuac uucaauaaga acacaggcaa auaugagauc     2940

cuuggacuca aguacagcga uaugcaauuu gaaaaaggca ccgggaagua uuccaucagc     3000

aaagagcagu acgagaauau uaaggugcgg gaaggagucg acgagaacuc ugaguucaag     3060

uuuacgcugu auaaaaauga ucugcuccug cuuaaggacu ccgaaaacgg cgaacagauc     3120

cugcuccgcu ucacaagcag aaaugauacc uccaaacacu acguggagcu gaagccuuau     3180

aaccggcaaa aauuugaggg gucugaauac cugauuaaga gccuuggaac cguugugaaa     3240

ggcgggcgcu gcaucaaggg ccugggaaag uccaauauuu cuaucuacaa agucagaaca     3300

gacgugcucg gcaaccagca uaucauuaag aaugaggggg auaaacccaa gcuggacuuc     3360

uga                                                                   3363


<210>  3938
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3938
augggccugg acaucggggu ggccagcguc ggauucuccg uucucgauau ugacaagggc       60

aaagugaucg agcugggggc uagacucuuu ucugccaccg uggcggcugg aaaccaggau      120

cggcgcgaca ugagaggcgc ccggcgccug cucaauagaa acaagcaacg gcgccaggau      180

acagggaaac uguucaagaa auuuggacuu auugacgauu ucgacaaggg cagcuuuuac      240

gauaauuuca accagaaucu gaaccccuau gaacucagag ucaagggccu gacggagcag      300

cugaccaaag aagaacucgc cgaaucccug uaccaaaucg ugaagcaccg ggggauuucu      360

uaugcucuua aagacgccga uguugacgag ggaggcacag auuacagcgu gucccugaag      420

aucaauucuc aggagcucgc ugagaaaacc ccugcccaga uccagcugca acgccuuaac      480

gacuauggga aggucagagg acaggugguu auuggcgacg auccggacaa ucagaaggug      540

cugcucaacg ucuuucccac aagcgcguac gagaaagaag ccaagcagau cauugcuacc      600

caacagcagu ucuauccuga gucccugaca gauaaauuua ccgaggaaua cugccaaauc      660

cugacgcgga agcgcgacua cuucgugggg cccggaaaug agaaaagcag aaccgauuau      720

ggcauuuaca agacagacgg ccggacccuc gauaaccugu uugaggaacu uaucgggcau      780

gacaaaauuu auccugagga acugcgcgcc ucugcugccu ccuacacagc gcagcuuuuc      840

aaugugcuga acgaucuuaa uaaccugaga auccucaacu augaggacgg aaagcugacc      900

aaggaagaua aagagaagau cauugccgaa aucaaaaaua acacgacaac cauuaauaug      960

cugaacguca ucaagaaagu ugcuggcugu agcaaggacg acauuaaggg guuucgggug     1020

aaugagaaag auaagcccga aaucucuucc augccugugu acagaaaaau ccacaaggac     1080

cuccugaaag ccggagucga uauuagcgac uggccggugg aguucaucga ugagcuuucu     1140

uucauucuga cacucaacac cgaaaauggc gagauccgca agcagcugaa caaucggcuu     1200

gcucccaagu uugacuuccu gaacgccgau cucauucagc ugaucaucga caauaaagau     1260

uccuuugaaa uuaagaccaa caauaaaugg cauagauuca gcguuaagac aaugaacaaa     1320

cugaucccug agaugaugga acgccccgug gagcaaauga cgcuccugaa ugagaugggg     1380

cuugucaaga aggacaaaaa gcgguuugaa aacaacaaau aucugccuua caaggagauu     1440

gccaaagaua ucuucaaucc cguggcuucc aagucuguua gagaagcgcu caaaauugug     1500

aacgccgucc ugaagaagua cggacacauc gacuaucugg uggucgagau gccgcgcgau     1560

aaaaaucuua aggaggaaca ggacaacauu aaagaguuuc agaauaagaa caaaaaggcu     1620

aaggacgccg ccuucgaggc uuuugugaaa agcguuggcu ccgaacagcg ggugaaggaa     1680

gcccucagca aaaauagaaa gcugcaaaug aaaaugcgcc ugugguacca gcagcaggag     1740

aucgauccuu auaacggcaa gaccaucgac gcgacagauc ucauuaauaa ccccgacaag     1800

uucgagaucg aucauauuau cccucaaucu auuuccuuuu acgacagcau caauaacaaa     1860

acccugugcu ucgcuucuau gaaucagguc aaggggcaga aaacacccua ugaauuuaug     1920

cuugagggac acggccagag cuacgauaag uucaaagcca ccgugauggc caacaagaau     1980

uucgggaagg cuaaacgggc caacuaucug uuugaagaga acguuuccga caucgaaacg     2040

agaaagcgcu uccucucucg gaaucuggug gauacaagau acagcucccg ggucgugcug     2100

aacagccuuc aagacuuuuu ccgcgagaaa ucugcugaua ccaaggugac cguuauuaga     2160

ggaaaauuua cauccaauau gcggaagcac uggcauaucg acaaaaccag agagacauuc     2220

caccaucacg ccauugaugc gagcaucauu gccgcuacgc ccuuucuccg cauguggaag     2280

aagggcggga ccaucuuccc ugucaaagug ggcgaagagu cuaucgacau ugaaacagga     2340

gagauccugg acgauaagaa cuuugacaaa gccauguacg aggaaccgua uuccggcuuc     2400

gugagcgaga uuaugaaugc ugaugaccgg aucaaguuuu cccaccaggu cgauaaaaag     2460

augaacagaa aggugucuga cgccaccauu uacagcacac gcaccgggaa acuggcgaag     2520

gauaaaaagg acgccgaaua uaucguugcu aaagugaagg auaucuacuc cgucgacgga     2580

uucaagaaau uuaagaaagu guaugauaag gacaaaacga aguuccuccu guacaaguau     2640

gaccccagaa ccuucucuaa acuugagcgg auuaucagcg auuguccuga caagguugaa     2700

aaagugcaga caaauggcaa ggucaaagcc guggauauuu cccccuuuga gauguaccgc     2760

agagaccaug ggaugaucaa gaaguacagc aaaaagggaa acggcccugc uauuaaacaa     2820

cugaaguauc ucgauaaaaa gcuggggucu cacaucgaca uuacccccgc caaugccaac     2880

ggcaaacaug uuauccugca gucccuuaag ccguggcgga cagaugugua ccucaaucac     2940

gagaccggag aauaugagau caugggcauu aaguacagcg accugaaauu caacaagaau     3000

gaaggguaug gaaucaaaaa ggauaaauac cuggagauua agaaggucga agaggugucu     3060

gagaaauccg aauuuauguu cagccucuau cgcaaggaca gagugaaagu ccaggauaug     3120

aagacgggcg agucuguuga acugcuguuu uggagccgga acuucuccaa uaaaaaguac     3180

gcugagcuua agccuaucag ccaggcggaa aacgacaaaa agcuccccgu guacgggaaa     3240

ggacgccuga uuaagagacu gaucccuaaa aacugcaaga ucuggaaggu gaauacaacc     3300

auucuuggcg aucccuauua ccucgagaaa gagucugacu ccccuaagga cauccuggau     3360

uga                                                                   3363


<210>  3939
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3939
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggugcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcagag uccggcugaa ucgccuuuuu gaggaaagcg ggcugaucac cgacuucaca      240

aagaucucca uuaaccucaa uccuuaccag cugagaguga aaggacugac cgaugagcuc      300

ucuaacgaag aacuguuuau cgcgcuuaag aauaugguua agcaccgggg cauuagcuau      360

cuggacgaug cuuccgacga uggcaacucu agcaucgggg acuacgccca gauugugaaa      420

gaaaauucca agcagcucga aacaaaaacc ccgggacaaa uccagcugga gcgcuaucag      480

acauacggcc agcuuagagg ggauuucacc gucgaaaagg acggaaagaa gcaucggcug      540

auuaacgugu uucccacgag cgccuaucgc ucugaggcuc ucagaauccu gcaaacccag      600

caggaguuca auccucagau cacagaugaa uuuguuaacc gguaccugga gauucucacc      660

ggcaagagaa aauauuacca cgggcccgga aaugaaaagu cccgcacaga cuacggccgg      720

uauagaacca acggcgagac gcuggauaac aucuucggga uucuuaucgg aaaaugcaca      780

uuuuacccug acgaguuccg cgccgcuaag gccagcuaua ccgcgcaaga guuuaaucug      840

cucaacgacc ugaauaaccu uacagugccc accgagacca aaaagcuguc uaaagaacag      900

aagaaucaga uuaucaacua cgucaagaau gagaaagcca ugggcccugc uaaacucuuc      960

aaauauaucg ccaagcugcu guccugugau guggcugaca uuaaagggua ccggaucgau     1020

aagagcggaa aggccgaaau ucauacauuu gaggcguaua gaaaaaugaa gacgcucgaa     1080

acccuggaca ucgagcaaau ggaucgcgag acacuugaca aacuggccua cgugcucacc     1140

cugaacacag aacgggaggg cauucaggaa gcucuugagc acgaguucgc cgaugggucu     1200

uucucccaga agcaggucga cgagcugguu caauuuagaa aagcuaauag cuccaucuuc     1260

ggaaagggcu ggcacaacuu uucugugaag cucaugaugg agcugauccc ggaacuguac     1320

gagaccagcg aggaacagau gacgauucuc acacgccugg gcaaacagaa gaccaccucc     1380

agcucuaaua aaacaaagua uaucgaugag aaacuucuga ccgaagagau uuacaacccc     1440

guggucgcca aguccgugcg gcaggccauc aagauuguua augcugcgau caaagaauau     1500

ggggacuucg auaacauugu gaucgagaug gccagagaaa caaaugagga cgacgagaag     1560

aaagcuaucc aaaagauuca gaaagccaac aaggaugaaa aagacgccgc uaugcucaag     1620

gccgcgaacc aguacaaugg aaaggcugag cugccucaua gcgucuuuca cggccauaaa     1680

cagcuggcca cgaagauccg ccuuuggcac caacaggggg aacggugccu cuauaccgga     1740

aaaacaauuu cuauccacga ucugauuaac aauagcaacc aguucgaggu ggaccauauc     1800

cugccccucu ccaucaccuu ugaugacucu cuggccaaua agguucuugu guacgcuaca     1860

gccaaccagg aaaaaggcca aagaaccccu uaucaggcuc uggauagcau ggacgaugcc     1920

ugguccuucc gggagcucaa ggcguuuguc cgcgagagca agacgcuguc uaauaaaaag     1980

aaagaauacc ugcuuaccga ggaagacauu uccaaguucg augugcggaa aaaguuuauc     2040

gagagaaacc ucgucgacac aagauacgcc agccgcgugg uucugaaugc ucugcaggaa     2100

cacuuccggg cccauaagau ugauaccaaa gugucugucg ugagagggca auuuacaucc     2160

cagcuccgca gacacugggg caucgagaag acccgggaca cguaucauca ccacgcuguu     2220

gacgcccuga uuaucgcggc cagcucccag cuuaaccugu ggaaaaagca gaaaaauaca     2280

cucgugucuu acagcgagga ucaacugcug gacaucgaaa ccggagagcu uauuuccgau     2340

gacgaauaua aggaguucgu cuucaaggcu cccuaccagc auuuugugga uacacucaaa     2400

ucuaaggagu ucgaagacag cauccuguuu uccuaucagg uggauagcaa auucaaccgc     2460

aagauuucug acgccaccau cuacgcuacc agacaggcca aaguuggcaa ggauaaagcc     2520

gacgagacau auguccuggg gaagauuaag gauaucuaca cgcaagacgg auacgaugcu     2580

uuuaugaaaa ucuauaagaa agacaagucc aaauuccuca uguaccggca cgacccgcag     2640

accuuugaaa aggugauuga gccuauccug gaaaauuauc ccaacaagca gaugaaugag     2700

aaaggcaagg aggugccuug uaaccccuuc cuuaaauaca aggaggaaca uggguauauu     2760

cgcaaauaca gcaagaaggg aaacggcccc gaaaucaaau cucugaagua uuacgauucc     2820

aaacucggga aucacauuga caucacaccu aaggauagca acaauaaagu cgugcugcag     2880

ucuguuagcc cguggagagc ggacguguac uuuaacaaua ccacaggcaa guaugagauu     2940

cugggacuua aguacgccga ucuccaauuc gaaaaaggca ccgggacgua uaagaucucc     3000

caggagaaau acaacgacau caagaaaaag gagggagucg auagcgacuc ugaauuuaag     3060

uucacacugu auaaaaauga ucugcuccug gugaaggaga ccgaaaccaa agagcagcaa     3120

cuuuuucggu uccugucccg cacaaugccc aagcagaaac acuacguuga acucaagccu     3180

uaugacaaac agaaguucga gggcggggag gcucugauua aggugcuggg aaacgucgcc     3240

aauggcgggc agugcaucaa aggccuugga aagagcaaca uuuccaucua caaagugaga     3300

accgauguuc ugggcaauca acauauuauc aagaacgaag gggacaaacc caagcucgac     3360

uuuuga                                                                3366


<210>  3940
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3940
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaaaauu ccggaaaaau uauccacgcu aacucuagac uguuccccgc cgcgaccgcu      120

gauaauaacg uggaacggcg caagaauaga caggcccggc gccucaacag acggaaaaag      180

caucgcggcg ugagauuuca agaccuguuc gaggauuacg ggcuucugac agacuuuagc      240

aaagucucca uuaaucucaa cccuuaucgg cugcgcgugg auggacugaa uaagcagcuc      300

acgaacgaag aacuguucau cgcccuuaag aauaucguua aaagacgggg cauuucuuac      360

cuggacgaug cuagcgaaga cggcgggacc guguccucug auuauggaaa ggccgucgag      420

gagaaccgca aacuccuggc ugaacagaca ccgggccaga uccaacuuga gagauuugaa      480

aaguacgggc agcugcgggg agacuucacc gugguugaga auggcgagaa augccgccuc      540

auuaacgugu uuagcacauc cgccuauaag aaggaagcgg agagaauccu gcggaaacag      600

caggaguuca auagcaagau uaccgaugag uuuaucgaag acuaccugaa aauucucaca      660

gggaagagaa aauauuacca cggacccggc aacgagaagu cucgcaccga uuacggccgg      720

uucagaaaag acgggacgac ccuggacaac aucuuuggaa uccuuauugg caaguguaca      780

uucuauccua augaguaccg cgccuccaag gcuagcuaua ccgcccaaga auuuaaccug      840

cucaaugauc ugaacaaucu uucugucccc acagagacca aaaagcuguc cgaggaacag      900

aaaaagacga ucauugaaua cgcuaaaagc gccaagacac ucggggcguc uacccugcug      960

aaguauaucg ccaaaaugau ugacacaucc guggaucaga uccggggaua cagaguggac     1020

gucaacaaua agccugagau gcauaccuuc gagguuuauc gcaaaaugca gagccucgaa     1080

accaucuccg ugggcgagcu gucucggaac gugcuugaug aacuggcuca cauucucaca     1140

cugaauacgg agagagaagg gaucgaggag gccauuaaca ccaaacuuaa ggacagcuuc     1200

ucccaagauc agguccugga acucgugcag uuucgcaaga auaacagcuc ucuguucucc     1260

aagggauggc auaauuuuag ccugaaacuc augauggagc ugauccccga acuuuacgag     1320

acaucugagg aacaaaugac cauucugaca cggcucggca agcagaaaag caaggagacc     1380

uccaaaagau cuaaguacau cgacgaaaag gagcugacgg aagagaucua uaacccuguu     1440

guggcuaaaa gcguccgcca ggccauuaag aucauuaaug aggccacaaa gaaguacggc     1500

aucuucgaua acauugugau cgaaauggcu cgggagaacg acgaagagga ugcgaaaaag     1560

gacuauauua aaagacagaa ggccaaucaa gacgaaaaga acgcugccau ggagaaagcc     1620

gcuuuucagu acaaugggaa gaaagagcug ccggauaaca ucuuccacgg acacaaggaa     1680

cuugccacca aaauccggcu cuggcaucag cagggcgaga agugccugua uaccgggaag     1740

acaauuccca ucuccgaccu gauucacaau caauacaaau augaaaucga ucauguucuc     1800

ccucugagcc uuucuuuuga cgauucccug agcaacaagg ugcucguccu ggcgaccgcu     1860

aaucaggaga aaggacagcg cacacccuuc caggcccugg acucuaugga ugacggcugg     1920

uccuacagag aauuuaagag cuacgugaaa gaguccaaac uucucgggaa caagaagaag     1980

gaguaucugc ugacggaaga ggauauuucu aaaaucgaag ucaagcaaaa auucaucgag     2040

cggaaucucg uggacaacag auacagcucc cgcguugugc ugaaugcccu ucaggauuuu     2100

uauaaggcuc acaaguucga caccacaauu ucugucgugc ggggccaguu uaccagccag     2160

cugagacgca aauggggacu cgagaagucc agagaaacau accaccauca cgccguugau     2220

gcucugauca uugccgcgag cucucaacug cggcuuugga aaaagcauaa caauccucuc     2280

aucuccuaua aagagggccg cuucauugac agcgaaaccg gggagaucau cucucugucc     2340

gacgaugaau acaaggagcu gguguucaaa gcccccuaug accacuuugu cgauacgcuc     2400

agaagcaaga ccuucgagga cucuauucug uuuagcuacc agguggauuc caaguacaac     2460

cggaaaauca gcgacgcuac aauuuaugcc acccgcaagg cuaaacuuga uaaggacaaa     2520

ucugaagaga cauacgugcu gggaaagauc aaggauauuu auucccaggc cggcuacgac     2580

gcguucauca aaaucuauaa uaaggauaaa agcaaguuuc ucauguacca uaaagacccg     2640

caaaccuucg aaaagguuau ugaggaaauc cugagaacgu auccuuccaa ggagcugaac     2700

gauaaaaaca aggagauucc cuguaauccu uuugaaaaau accggcagga gaacgggccc     2760

auccgcaagu acucuaaaaa gggaaauggc cccgaaauua agagcaugaa auauuacgac     2820

aagaacaucc cugaguccuu cauuaauauc acaccgucua acagcaauaa caaaguccuu     2880

cucagacagc uguaucccug gcggaccgac guguacuuua auccucagac agauaaguau     2940

gaacugcucg ggcugaaaua cgccgaccuu caauucgaga aggcuaccgg caaguauucc     3000

aucaccauug auaaauacaa cgagaucaag gaaaaagagg gagugucuaa ggaaagcgag     3060

uuuaaauuca cacuguacaa gaaugaccuc cugcugauua aagauacgaa gaacaaugag     3120

gaacagaucu uucgcuuccu uuccagaacc augcccaacc agaaucacua ugucgagcug     3180

aagccuuacg acaaaggcaa cuucgaaggg aaggagaaaa uuaagguguu uaaaaugaca     3240

agcaacggac ggugcaucaa gggccucggg aagaaauauc ugucuaucua caagguuaaa     3300

accgauauuc ucggaaauaa gcacuucauc aaaaaggaag gcgacgagcc caagcugaaa     3360

uuuuga                                                                3366


<210>  3941
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3941
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaagaaua gcggaaaaau uauccacgcu aacucuagac uguuccccgc cgcgaccgcu      120

gauaauaacg uggaacggcg caagaauaga caggcccggc gccucaacag acggaaaaag      180

caucgcggcg ugagauuuca agaccuguuc gaggauuacg ggcuucugac agacuuuagc      240

aaagucucca uuaaucucaa cccuuaucgg cugcgcgugg auggacugaa ucagcagcuc      300

acgaacgaag aacuguucau cgcccuuaag aauaucguua agagacgggg cauuucuuac      360

cuggacgaug cuagcgaaga cggcggggcc guguccucug auuauggaaa agcugucgag      420

gagaaccgca agcuccuggc cgaacagacc ccgggccaaa uccagcuuga gagauuugaa      480

aaauacggac aggugcgggg agacuucaca guuguggaga auggcgagaa gcgcagacug      540

auuaacgucu uuagcaccuc cgcguaucgg aaagaagccg agaagauccu cagaaagcag      600

caagaguuca auaaccagau uacagaugag uuuaucgaag acuaccugaa aauucugacc      660

gggaagcgca aauauuacca cggacccggc aacgagaagu cccggacaga uuacggcaga      720

uuccgcaccg acgggacgac ccucgacaau aucuuuggag ugcugaucgg caaaugcaca      780

uucuauccua acgaguaccg ggcuucuaag uccagcuaua ccgcccagga auuuaaucuu      840

cugaacgauc ucaauaaccu gacagugccc accgagacga aaaagcuguc ugaagaacag      900

aagaaaguca uuguugaaua cgcuaaggag gccaaaacac uuggggcguc cacccuccug      960

aaguauaucg ugaaaaugau ugacgccagc guggaucaaa ucucuggaua cagagucgac     1020

gugaauaaca agccugagau gcauacauuc gaaguuuauc gcaagaugca gucccuggag     1080

accauuagcg ugggcgaacu cucccggaau guccuggaug agcuugcuca cauccugacc     1140

cucaacacag aaagagaggg gaucgaggaa gccauuaauu cuaaacugau gggaguguuc     1200

agccaggacc aaguucuuga gcuggugcag uuucgcaaga acaauuccag ccucuucucu     1260

aaaggcuggc auaacuuuuc ccugaagcug augauggaac ucauccccga gcuguacgag     1320

acgagcgagg agcagaugac cauucuuaca cggcugggca aacagaaguc uaaggaaacc     1380

agcaaaagaa caaaguacau cgaugagaaa gaacucaccg aggagauuua uaauccuguc     1440

guggcuaagu ccguccgcca agccaucaaa aucauuaacg aagccacgaa gaagcacggg     1500

aucuucgaca acauugugau cgagauggcu cgggaaaaua acgaggaaga ugcgaaaaag     1560

gacuacauua aaagacagaa ggccaaucag gaugagaaaa acgcugccau ggagaaggcc     1620

gcuuuucagu auaaugggaa aaaggaacug ccgggcaaca ucuucaaugg gcacaaggag     1680

cuggccacaa aaauucggcu uuggcaucaa cagggagaaa agugucucua caccggcaaa     1740

accaucccca ucucugaccu gauucacaac caguauaagu acgagaucga ccauauucug     1800

ccucucagcc uguccuuuga ugacagccuu ucuaauaaag uucuggugca cgcgacagcu     1860

aaccaggaaa aggggcaacg cacccccuuc caggcccucg auuccaugga cgaugccugg     1920

agcuauagag aguuuaaguc uuacgucaaa gaccugaagu cccugagcaa uaaaaagaaa     1980

gauuaccuuc ucacagagga agacaucucc aagauugagg ugaagcagaa auucaucgaa     2040

cggaaccugg uugauacgag auauucuagc cgcguggucc ugaaugcucu ccaggacuuu     2100

uacaaguccc accaauucga uaccacaauc ucuguggugc ggggccaguu uaccagccag     2160

cugagacgca aauggggacu ugagaagucc agagagacau aucaucacca ugccguugac     2220

gcucugauua ucgccgcgag cucucaacuc cggcugugga aaaagcagaa caauccucug     2280

auuaccuaca aggaaggcca guucaucgau ucccagacgg gggagauuau cagccuuacc     2340

gacgacgaau auaaagagcu cgucuucaag gcccccuacg aucacuuugu ggacacacug     2400

cauucuaaaa ccuucgaaga uggaauccug uuuuccuauc aaguggacag caaguacaac     2460

cgcaaaauuu cugaugcuac aaucuacgcc accagaaagg cuaaacucga caaggauaag     2520

agcgaggaga cguauguccu gggcaaaauu aaggacaucu acucccaggc cggguaugau     2580

gcguucauua aaaucuacaa caaggacaaa cggaaguuuc uuauguauca caaggauccg     2640

cagacauucg aaaaagugau cgaggaaauu cugcgcaccu acccuagcaa ggagagaaau     2700

gacaaaaaca aggaaguucc cugcaauccu uuugagaaau aucggcagga gaacggaccc     2760

auccgcaagu acucuaagaa aggcaauggg cccgaaauua agugucucaa auacuaugau     2820

aacaagcugg gcaauuacau cgacauuucc ccugacggaa gcgauaacca agugguccug     2880

cagucccuua aaccguggag aacagacgug uauuucaauc accagaccaa gaaguacgag     2940

cucaugggcc ugaaauauuc ugaucugagc uuugaaaagg gguccggaaa auacucuauc     3000

agcaacgaga aguauaauuc cauuaaacgg aucgaaggcg uuaacgagca gucugaguuc     3060

aaguuuaccc ucuacaagaa ugaccugauc cuuauuaaag auagcgaaaa caaugagcaa     3120

aagcuguucc gcuuuaacuc cagaaacgac acagccaaac auuacgugga acucaagccc     3180

uaugauaaag cucgguucga cgggcagcag gagcugauca cgauucuggg aaaugucgcc     3240

aagggcgggc aaugccuuaa aggccugaac aagagcaaua ucucuauuuu caaggugaaa     3300

accgauguuc ucggaaagaa acacaucauc aagaaagagg gcgacgaacc uaagcugaag     3360

uuuuga                                                                3366


<210>  3942
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3942
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggugcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcaucg ucagacugaa ucggcuuuuu gaggaaagcg ggcugaucac cgacuucaca      240

aagauuucca ucaaccucaa uccuuaccag cugcgcguga aaggacugac cgaugagcuc      300

ucuaacgaag aacuguuuau ugcgcuuaag aauaugguua agcacagagg caucagcuau      360

cuggacgaug cuuccgacga uggcaacucu agcguggggg acuacgccca gauugucaaa      420

gaaaauucca agcagcucga aacaaaaacc ccgggacaaa uccagcugga gcgguaucag      480

acauacggcc agcuucgcgg ggauuucacc guggaaaagg acggaaagaa gcauagacug      540

auuaacguuu uucccacgag cgccuaucgg ucugaggcuc uccgcauccu gaagacccaa      600

caggaguuca auccucagau cacagaugaa uuuauuaaca gauaccugga gauccucacc      660

ggcaaacgga aguauuacca cgggcccgga aaugaaaaau ccagaacaga cuacggccgc      720

uaucggacca acggcgagac gcuggauaac auuuucggga uccuuauugg aaagugcaca      780

uuuuacccug aagaguucag agccgcuaaa gccagcuaua ccgcgcagga guuuaaucug      840

cucaacgacc ugaauaaccu uacagugccc accgaaacca agaaacuguc uaaggagcaa      900

aagaaucaga ucaucaacua cgucaaaaau gaaaaggcca ugggcccugc uaaacucuuc      960

aaguauauug ccaaacugcu guccugugac guggcugaua ucaaggggua ccgcauugac     1020

aagagcggaa aagccgagau ccauacauuu gaagcguauc ggaagaugaa aacgcucgag     1080

acccuggaua uugagcagau ggacagagaa acacuugaua agcuggccua cgugcucacc     1140

cugaacacag agcgcgaagg cauccaagag gcucuugaac acgaguucgc cgacgggucu     1200

uucucccaga aacaggucga ugagcugguu caguuucgga aggcuaauag cuccaucuuc     1260

ggaaagggcu ggcacaacuu uucugugaaa cucaugaugg aacugauucc ggagcuguac     1320

gaaaccagcg aggagcaaau gacgauccuc acaagacugg gcaagcagaa aaccaccucc     1380

agcucuaaua agacaaaaua uauugacgaa aaacuucuga ccgaggaaau cuacaacccc     1440

guggucgcca aguccgugcg ccaggccauu aaaaucguua augcugcgau uaaggaguau     1500

ggggauuucg acaacaucgu gaucgaaaug gcccgggaga caaaugagga ugacgagaaa     1560

aaggcuauuc agaaaaucca aaaggccaac aaagacgaga aggaugccgc uaugcucaag     1620

gccgcgaacc aguacaaugg aaaagcugaa cugccucaua gcgucuuuca cggccauaag     1680

cagcuggcca cgaaaauuag acuuuggcac cagcaagggg agcgcugccu cuauaccgga     1740

aagacaaucu cuauucacga ccugaucaac aaucccaacc aguucgaagu ggaucauauc     1800

cugccucuca gcauuaccuu ugacgauucc cuggccaaua aaguucuugu guacgcuaca     1860

gccaaccagg agaagggcca gcggaccccc uaucaagcuc uggacucuau ggaugacgcc     1920

uggagcuuca gagagcucaa ggcguuuguc cgggaaucca aaacgcugag caauaagaaa     1980

aaggaguacc ugcuuaccga agaggauauc ucuaaauucg acgugcagaa gaaguuuauu     2040

gaacgcaacc ucgucgauac aagauacgcc ucccgggugg uucugaaugc ucugcaggag     2100

cacuucagag cccauaaaau cgacaccaag gugagcgucg ugcgcgggca auuuacaucu     2160

cagcuccgga gacacugggg cauugagaaa acccgcgaua cguaucauca ccacgcuguu     2220

gacgcccuga ucaucgcggc cuccagccag cuuaaccugu ggaagaaaca gaagaauaca     2280

cucguguccu acucugaaga ccaacugcug gauauugaga ccggagaacu uaucagcgac     2340

gaugaguaua aggaguccgu cuucaaagcu ccguaccagc auuucgugga cacacucaag     2400

ucuaaagaau uugaggauag cauucuguuc uccuaucagg uggacagcaa guuuaacaga     2460

aaaaucucug augccaccau uuacgcuacc cggcaggcca agguuggcaa agacaagaag     2520

gaugaaacau auguccuggg gaaaaucaag gacaucuaca cgcaagaugg auacgacgcc     2580

uucaugaaaa uuuauaagaa agauaagucc aaguuucuca uguaccgcca cgacccucag     2640

accuucgaga aagugaucga acccauucug gagaauuauc cuaacaagga gaugaaugaa     2700

aaaggcaagg aggugcccug uaaccccuuu cuuaaauaca aggaggagca uggguauauc     2760

agaaaguaca gcaaaaaggg aaacggcccu gaaauuaaau cucugaagua uuacgacucc     2820

aaacucggga aucacaucga uauuacaccg aagaacagca auaacaaggu cgugcugcag     2880

ucuguuagcc ccuggcgggc ugacguguac uucaauaaaa ccacaggcaa guaugagauc     2940

cugggacuua aauacgcgga ucuccaguuu gagaagggca ccgggacgua uaaaaucucc     3000

caagaaaagu acaacgacau uaagaaaaag gagggagucg auagcgacuc ugaguucaaa     3060

uuuacacugu auaagaauga ucugcuccug gugaaagaca ccgaaaccaa ggaacagcag     3120

cuuuuccgcu uucuguccag aacaaugccu aaaaagaagc acuacguuga gcucaaaccc     3180

uaugauaagc aaaaauucga gggcggggaa gcccugauca aggugcuggg aaacgucgcu     3240

aauagcggcc agugcauuaa agggcuugcc aaguccaaca ucucuauuua caaggugcgg     3300

accgacguuc ugggcaauca gcauaucauc aaaaacgagg gagauaagcc uaaacucgac     3360

uucuga                                                                3366


<210>  3943
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3943
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggugcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcagag uccggcugaa ucgccuuuuu gaggaaagcg ggcugaucac cgacuucaca      240

aagaucucca uuaaccucaa uccuuaccag cugagaguga aaggacugac cgaugagcuc      300

ucuaacgagg agcuguuuau cgcgcuuaag aauaugguua agcaccgggg cauuagcuau      360

cuggacgaug cuuccgacga uggcaacucu agcaucgggg acuacgccca gauugugaaa      420

gaaaauucca agcagcucga aacaaaaacc ccgggacaaa uccagcugga gcgcuaucag      480

acauacggcc agcuuagagg ggauuucacc gucgaaaagg acggaaagaa gcaucggcug      540

auuaacgugu uucccacgag cgccuaucgc ucugaggcuc ucagaauccu gcaaacccag      600

caggaguuca auccucagau cacagaugaa uuuauuaacc gguaccugga gauccucacc      660

ggcaagagaa aauauuacca cgggcccgga aaugaaaagu cccgcacaga cuacggccgg      720

uauagaacca gcggcgagac gcuggauaac auuuucggga uccuuauugg aaaaugcaca      780

uuuuacccug acgaguuccg cgccgcuaag gccucuuaua ccgcgcaaga auuuaaccug      840

cucaaugacc ugaacaaucu uacaguuccc accgagacca aaaagcuguc caaagaacag      900

aagaaccaga ucaucaauua cgugaagaac gagaaagcca ugggcccugc uaaacucuuc      960

aaauauauug ccaagcugcu gagcugugau gucgcugaca ucaaagggua ccggauugau     1020

aagucuggaa aggccgaaau ccauacauuu gaggcguaua gaaaaaugaa gacgcucgaa     1080

acccuggaca uugagcaaau ggaucgcgag acacuugaca aacuggccua cgugcucacc     1140

cugaauacag aacgggaggg cauccaggaa gcucuugagc acgaguucgc cgaugggucc     1200

uucagccaga aacaggugga cgaacugguc caauuuagaa aagcuaacuc cucuaucuuc     1260

ggaaagggcu ggcacaauuu uagcguuaag cucaugaugg agcugauucc ggaacuguac     1320

gagaccuccg aggaacagau gacgauccuc acacgccugg gcaaacagaa gaccaccucu     1380

agcuccaaca aaacaaagua uauugaugag aaacuucuga ccgaagagau cuacaauccc     1440

gugguggcca agagcguccg gcaggccauu aagaucguga acgcugcgau uaaagaauau     1500

ggggacuucg auaauaucgu uaucgagaug gccagagaaa caaacgagga cgacgagaag     1560

aaagcuauuc aaaagaucca gaaagccaau aaggaugaaa aagacgccgc uaugcucaag     1620

gccgcgaacc aguacaacgg aaaggcugag cugccucauu cuguguuuca cggccauaaa     1680

cagcuggcca cgaagauucg ccuuuggcac caacaggggg aacggugccu cuauaccgga     1740

aaaacaauca gcauucacga ucugaucaau aacuccaauc aguucgaggu cgaccauauc     1800

cugccccucu cuauuaccuu ugaugacagc cuggccaaca aggugcuugu uuacgcuaca     1860

gccaaucagg aaaaaggcca aagaaccccu uaucaggcuc uggauuccau ggacgaugcc     1920

uggagcuucc gggagcucaa ggcguuugug cgcgagucua agacgcuguc caacaaaaag     1980

aaagaauacc ugcuuaccga ggaagacauc agcaaguucg augucagaaa gaaguuuauu     2040

gagcggaauc ucguggacac aagauacgcc ucucgcgucg ugcugaacgc ucugcaggaa     2100

cacuuccggg cccauaagau cgauaccaaa guuuccgugg ucagagggca auuuacaagc     2160

cagcuccgca gacacugggg cauugagaag acccgggaca cguaucauca ccacgcugug     2220

gacgcccuga ucaucgcggc cuccucucag cuuaaucugu ggaaaaagca gaaaaacaca     2280

cucguuagcu acuccgagga ucaacugcug gacauugaaa ccggagagcu uaucucugau     2340

gacgaauaua aggagagcgu guucaaggcu cccuaccagc auuucgucga uacacucaaa     2400

uccaaggagu uugaagacag cauucuguuc ucuuaucagg uggauuccaa auuuaaucgc     2460

aagaucagcg acgccaccau uuacgcuacc agacaggcca aagugggcaa ggauaaagcc     2520

gacgagacau auguucuggg gaagaucaag gauaucuaca cgcaagacgg auacgaugcu     2580

uucaugaaaa uuuauaagaa agacaagucu aaauuucuca uguaccggca cgacccgcag     2640

accuucgaaa aggucaucga gccuauucug gaaaacuauc ccaauaagca gaucaacgag     2700

aaaggcaagg aggugccuug uaaucccuuu cuuaaauaca aggaggaaca uggguauauu     2760

cgcaaauacu ccaagaaggg aaacggcccc gaaaucaaaa gccugaagua uuacgauucu     2820

aaacucggga accacauuga caucacaccu aaggauagca auaacaaagu gguccugcag     2880

uccgugagcc cguggagagc ggacguuuac uucaauaaga ccacaggcaa guaugagauc     2940

cugggacuua aauacgccga ucuccaauuu gaaaagggca ccgggacgua uaaaauuucu     3000

caggagaagu acaacgacau caaaaagaag gagggagugg auuccgacag cgaguucaaa     3060

uuuacacugu auaagaauga ucugcuccug gucaaagaca ccgaaaccaa ggaacagcaa     3120

cuuuuccggu uucugucccg cacaaugccc aaacagaagc acuacgugga gcucaaaccu     3180

uaugauaagc agaaguucga aggcggggag gcucugauua aaguucuggg aaacguggcc     3240

aauucuggcc agugcaagaa agggcuuggc aagagcaaca ucuccauuua caaagucaga     3300

accgacgugc ugggaaauca acauaucauc aagaacgagg gcgacaagcc caaacucgau     3360

uucuga                                                                3366


<210>  3944
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3944
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggugcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcagag uccggcugaa ucgccuuuuu gaggaaagcg ggcugaucac cgacuucaca      240

aagaucucca uuaaccucaa uccuuaccag cugagaguga aaggacugac cgaugagcuc      300

ucuaacgagg agcuguuuau cgcgcuuaag aauaugguua agcaccgggg cauuagcuau      360

cuggacgaug cuuccgacga uggcaacucu agcguggggg acuacgccca gaucgucaaa      420

gaaaauucca agcagcucga aacaaaaacc ccgggacaaa uucagcugga gcgcuaucag      480

acauacggcc agcuuagagg ggauuucacc guggaaaagg acggaaagaa gcaucggcug      540

aucaacguuu uucccacgag cgccuaucgc ucugaggcuc uccggauucu gcaaacccag      600

caggaguuca auccucagau cacagaugaa uuugugaaca gauaccugga gauccucacc      660

ggcaagagaa aauauuacca cgggcccgga aaugaaaagu cccgcacaga cuacggccgg      720

uauagaacca acggcgagac gcuggauaac auuuucggga uccuuauugg aaaaugcaca      780

uuuuacccug aagaguuccg cgccgcuaag gccagcuaua ccgcgcaaga guuuaaucug      840

cucaacgacc ugaauaaccu uacagucccc accgaaacca aaaagcuguc uaaagagcag      900

aagaaucaga ucauuaacua cgugaagaau gaaaaagcca ugggcccugc uaaacucuuc      960

aaauauaucg ccaagcugcu guccugugac guggcugaua ucaaagggua ccggauugac     1020

aagagcggaa aggccgagau ccauacauuu gaagcguaua gaaaaaugaa gacgcucgag     1080

acccuggaua uugagcaaau ggaccgcgaa acacuugaua aacuggccua cguccucacc     1140

cugaacacag agcgggaagg cauccaggag gcucuugaac acgaguucgc cgacgggucu     1200

uucucccaga agcagguuga ugagcuggug caauuuagaa aagcuaauag cuccauuuuc     1260

ggaaagggcu ggcacaacuu uucugugaag cucaugaugg aacugauccc ggagcuguac     1320

gaaaccagcg aggagcagau gacgauccuc aaacgccugg gcaagcagaa aacaaccucc     1380

agcucuaaua agaccaaaua uauugacgaa aaacuucuga cagaggaaau cuacaacccc     1440

gucguggcca aguccauucg gcaggccauc aaaauuguua augcugcgau caaggaguau     1500

ggggauuucg acaacauugu gaucgaaaug gccagagaga ccaaugagga ugacgaaaag     1560

aaggcuaucc agaaaauuca aaaggccaac aaagacgaga aggaugccgc uaugcucaag     1620

gccgcgaacc aguacaaugg aaaagcugaa cugccucaua gcgucuuuca cggccauaag     1680

cagcuggcca caaaaauccg ccuuuggcac caacaggggg agcggugccu cuauacggga     1740

aagaccauuu cuauccacga ccugauuaac aaucccaacc aguucgaagu ggaucauauc     1800

cugccucuca gcaucacauu ugacgauucc cuggccaaua aaguucuugu guacgcuacc     1860

gccaaccagg agaagggcca aagaacaccc uaucaggcuc uggacucuau ggaugacgcc     1920

uggagcuucc gggagcucaa ggcguuuguc cgcgaaucca aaacccugag caauaagaaa     1980

aaggaguacc ugcuuacgga agaggauauu ucuaaauucg acgugagaaa gaaguuuauc     2040

gaacggaacc ucgucgauac cagauacgcc ucccgcgugg uucugaaugc ucugcaggag     2100

cacuuccggg cccauaaaau ugacacaaag gugagcgucg ugagagggca auuuaccucu     2160

cagcuccgca gacacugggg caucgagaaa acacgggaua ccuaucauca ccacgcuguu     2220

gacgcccuga uuaucgcggc cuccagccag cuuaaccugu ggaagaaaca gaagaauacg     2280

cucguguccu acucugaaga ccaacugcug gauaucgaga caggagaacu uauuagcgac     2340

gaugaguaua aggaguccgu cuucaaagcu ccguaccagc auuucgugga cacccucaag     2400

ucuaaagaau uugaggauag cauccuguuc uccuaucagg uggacagcaa guuuaaccgc     2460

aaaauuucug augccacaau cuacgcuacc agacaggcca agguuggcaa agacaagaag     2520

gaugaaaccu auguccuggg gaaaauuaag gacaucuaca cacaagaugg auacgacgcc     2580

uucaugaaaa ucuauaagaa agauaagucc aaguuucuca uguaccggca cgacccucag     2640

acguucgaga aagugauuga acccauccug gagaauuauc cuaacaagga gaugaaugaa     2700

aaaggcaagg aggugcccug uaaccccuuu cuuaaauaca aggaggaaca uggguauauu     2760

cgcaaguaca gcaaaaaggg aaacgacccu gaaaucaaau cucugaagua uuacgauucc     2820

aaacucggca aucacauuga caucaccccg aaggauagca acaauaaggu cgugcugcag     2880

ucuguuagcc ccuggagagc ugacguguac uucaacaaaa caaccgggaa guaugagauu     2940

cugggccuua aauacgcgga ucuccaguuu gagaagggaa caggcaccua uaaaaucucc     3000

caagaaaagu acaaugacau caagaaaaag gagggggucg auagcgacuc ugaguucaaa     3060

uuuacgcugu auaagaacga ucugcuccug gugaaagaca cagaaaccaa ggaacagcag     3120

cuuuuccggu uucugucccg caccaugccu aaacaaaagc acuacguuga gcucaagccc     3180

uaugauaaac agaaguucga gggaggcgaa gcccugauua aagugcuggg gaaugucgcu     3240

aacagcggac agugcaagaa aggccuuggg aaguccaaua ucucuauuua caaggugaga     3300

acagacguuc ugggcaacca gcauaucauu aaaaaugagg gagacaagcc uaaacucgau     3360

uucuga                                                                3366


<210>  3945
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3945
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaaaauu ccggaaaaau uauccacgcu aacucuagac uguuccccgc cgcgaccgcu      120

gauaauaacg uggaacggcg caagaauaga caggcccggc gccuccauag acggaaaaag      180

caccgcggcg ugagauuuca agaccuguuc gaggauuacg ggcuucugac agacuuuagc      240

aaagucucca uuaaccucaa uccuuaucgg cugcgcgugg auggacugaa ccagcagcuc      300

acgaaugaag aacuguucau cgcccuuaag aacaucguua agagacgggg cauuucuuac      360

cuggacgaug cuagcgaaga cggcgggacc guguccucug auuauggaaa agccgucgag      420

gagaaucgca agcuccuggc ugaacagaca ccgggccaaa uccagcuuga gagauuugaa      480

aaauacggac aggugcgggg agacuucacc guuguggaga acggcgagaa gcgcagacug      540

auuaaugucu uuagcacauc cgccuaucgg aaagaagcgg agagaauccu ccgcaagcag      600

caagaguuca acagcaagau uaccgaugag uuuaucgaag acugccugaa aauucugaca      660

gggaagcgga aauacuauca uggacccggc aaugagaagu cuagaaccga uuacggccgc      720

uuccggacgg acgggaccac acucgacaac aucuuuggaa uccugauugg caaauguacc      780

uucuacccug augaguauag agccuccaag gcuagcuaca cagcccagga auuuaaccuu      840

cugaaugacc ucaacaaucu gaccgugccc acggagacaa agaaacuuuc ugaggaacag      900

aagaaaguga ucgucgaaua ugcuaaggag gccaaaaccc ugggggcguc cacacuccug      960

aaguacauug ccaaaaugau cgaugcuagc guugaccaga uucgcggaua ucggguggau     1020

gugaacaaua agccugagau ccacaccuuc gaagccuaca gaaagaugca aucucuggag     1080

accaucuccg ucggcgaacu cagccgcaac gugcuggacg agcuugcuca uauucugaca     1140

cucaauacgg aacgggaggg gaucgaggaa gccauuaaca ccaaacugaa ggauuccuuc     1200

ucucaggacc agguucuuga gcuggugcaa uuuagaaaga auaacagcuc ccucuucagc     1260

aagggauggc acaauuuuuc ucugaaacug augauggaac ucauccccga gcuguaugag     1320

acauccgagg aacagaugac cauucuuaca cgccugggca agcaaaagag caaagaaauc     1380

ucuaagcgga ccaaauacau cgaugagaag gaacucacgg aggagauuua caacccuguc     1440

guggccaaaa gcguuagaca ggcuaucaag auuaucaaug aagcgacaaa gaaacacggc     1500

auuuucgaca acaucgugau ugagauggcc cgggaaaaua acgaggaaga ugcuaagaaa     1560

gacuauauca agcgccagaa agccaaccag gacgagaaga augccgcuau ggagaaagcc     1620

gcguuucagu acaacgggaa gaaggaacug ccggauucca ucuuccaugg acagaaagag     1680

cuggcuacca agauuagacu uuggcaccaa cagggcgaaa aaugccucua uaccgggaag     1740

aauaucccca uuucugaccu gauccauaac caguacaaau augagauuga ucacauccug     1800

ccucucagcc uguccuuuga cgauagccuu ucuaauaagg uccuggugcu cgccacagcc     1860

aaccaggaaa agggacaacg gacccccuuc caggcucugg acuccaugga ugacgccugg     1920

agcuacagag aguuuaaauc uuaugucaag gaguccaaac ugcuuggcaa uaagaaaaag     1980

gaauaccucc ugacagagga agauaucagc aagauugagg ugaaacagaa guucaucgag     2040

cgcaaccuga uugacacgcg guacuccucu agaguugugc ucaaugcucu gcaggauuuu     2100

uauaaagccc acaaguucga caccacaauc agcgucgugc gcgggcaauu uaccucccag     2160

cuuagacgga aauggggccu ggaaaagucu cgcgagacau accaucacca ugcgguugau     2220

gcccucauua ucgcugccag cucccagcug agacugugga agaaacaaaa caauccucuu     2280

aucgcuuaua aggaaggaca guucguggac agcgagaccg gcgaaauuau cucucucucc     2340

gacgaugagu acaaagagcu ggucuucaag gcccccuaug accacuuugu ggauacgcug     2400

agcucuaaaa ccuucgaaga cuccauucuc uuuagcuacc agguggauuc uaaguucaac     2460

cggaaaauca gcgacgcgac aauuuaugcc acccgcaagg cuaagcugga uaaagagaag     2520

uccgaagaga cauacguucu ugggaaaauc aaggacaucu acagccaggc cggauaugau     2580

gcuuuuauua aaaucuacaa uaaggacaag ucuaaauucc ugauguauca uaaggauccg     2640

caaaccuuug aaaaagucau ugaggagauc cucagaacgu acccuuccaa ggaacugaac     2700

gacaaaaaua aggagauucc cuguaacccu uucgaaaagu aucggcagga gaacggcccc     2760

auccgcaaau acagcaagaa auccaauggg cccgaaauua agucucugaa auauuacgau     2820

aacaaacuug gaaaucacau cgacaucaca ccugagagcu ccgacaacca gguggugcuc     2880

cagucucuga agccguggag aaccgauguc uacuuuaauc accaaacaaa gaaguaugag     2940

cugaugggcc ucaaauacag cgaccugucc uucgaaaagg ggucuggcaa auauagcauu     3000

uccaaccaga aguacaauag caucaagcgg auugagggaa ucgaugaaca gucugaguuu     3060

aaauucaccc uuuauaagaa cgaccugauu cucaucaaag auuccgagaa uaacgaacag     3120

aagcuguuuc gcuucaauag cagaaacgac accgccaaac auuacaucga gcugaagccc     3180

uaugauaaag ccaaguucga cggcgaacaa gagcuuauua caauccuggg gaauguggcu     3240

aagggaggcc ggugccucaa agggcugaac aagucuaaua uuuccaucuu uaaaguuaag     3300

acggaugugc ucgggaaaaa gcacauuauc aagaaagaag gcgacgagcc uaagcugaaa     3360

uucuga                                                                3366


<210>  3946
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3946
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

aauaaggaua ccggagagau uauccacaca aacuccagaa uuuuccccgc ugccacggcg      120

gacaauaacg uggaacggcg caauucuaga caggaucggc gccugaacag acggaaaaag      180

caucgcggcg ugagacucca cgaccuguuu gaggauuacg accuucugac cgauuucagc      240

aaagucucca ucaaucucaa cccuuaucgg cugcgcgugc aagggcugga caauucucuc      300

acaaccgaag aacuguuuau cgcucuuaag aacauuguua aaagacgggg aaucagcuac      360

cuggaugacg ccuccgaaga uggcggcgug gucucuagcg acuaugggaa ggccguggag      420

gagaaucgca agcuccuggc ugaacagaca ccgggacaga uucagcuuga gagauucaaa      480

aaguacggcc aaguuagagg ggauuuuacc guggucgaaa acggagagaa acgcagacug      540

aucaaugugu ucuccacaag cgccuaucgg aaggaggcug aacggauucu ccgcaaacag      600

caggaguuua acaaucagau caccgacaag uucauugaag acuaccugaa gauccugacg      660

gagaaacgga aguauuacca uggccccggg aacgaaaaau cuagaaccga uuacggacgc      720

uuucggacag acggcaccac acucgauaac aucuucggca uucugaucgg gaagugcacc      780

uuuuauccua augaguacag agccuccaaa gcgagcuaua cggcccaaga guucaaccuu      840

cugaaugacc ucaacaaucu gacagugccc accgaaacaa agaaacuuuc ugaggaacag      900

aagaagacca uugucgagua cgcuaaaucc gccaagaccc ugggagcuag cacacuccug      960

aaauauaucg ccaagaugau ugaugcgucu guugaccaga uccgcggcua ccggguggau     1020

gugaacaaua aaccugaaau gcacacguuu gaggucuaua gaaagaugca gucccuggag     1080

accaucagcg ugugggaacu cucccgcaac auucuggacg agcuugccca uauccugaca     1140

cucaauaccg aacgggaggg gauugaagag gcuaucaaca caaagcugau ggauguuuuc     1200

ucucaagacc aggugcuuga gcugguccag uuccgcaaaa auaacagcuc ccucuuuagc     1260

aagggauggc acaauuucuc uagaaaacug augauggaac ugauucccga gcucuacgaa     1320

accuccgagg agcaaaugac gauccugaca cggcuuggca agcagaaaag caaggaaacc     1380

ucuaagagaa ccaaauacau cgaugagaag gaagugacag aggaaauuua uaacccuguu     1440

guggccaaaa gcguccggca ggcuaucaag auuaucaaug aggccaccaa aaaguacggg     1500

auuuuugaca acaucgugau ugagauggcc cgcgaaaaca augaggaaga cgcuaagaaa     1560

gauuauauca agagacagaa agcgaaccaa gacgagaaga augucgccau cgaaaaagcu     1620

gccuuccagu acaacggaaa gaaagagcug ccggauucca uuuuucacgg ccauaaggag     1680

cucgccacaa agauccggcu guggcaccag cagggcgaaa aaugucugua uacggggaag     1740

aauauuccca ucucugaccu uauucauaac caauacaaau augagaucga ucacauccuc     1800

ccucugagcc uguccuucga cgauagccuc ucuaauaagg ugcugguucu ugcuaccgcc     1860

aaccaggaaa aaggacagag aacacccuuu caggcgcugg acuccaugga ugacgcuugg     1920

agcuaccgcg aguucaaguc uuacgugaag gauuccaaac uccugggcaa uaagaaaaag     1980

gacuaucugc uuaccgaaga ggauauuagc aaaaucgagg ucaagcaaaa guuuauugaa     2040

cggaaccucg uggacacaag auacuccucu cgcguugugc ugaauacccu gcaggacuuc     2100

uauaaagccc acaaguuuga uacgaccauc agcgucguga gagggcaguu cacaucccag     2160

cuccggcgca aauggggacu ggagaagucu agagaaaccu accaucacca ugccguggac     2220

gcucuuauua ucgccgcuag cucccaacug cggcucugga aaaagcaggc caacccucug     2280

aucagcuaua aggaggauca guuuguugac ucugagacag gcgaaauuau cucccugacc     2340

gaugacaaau acaaggagcu ugucuucaaa gcgcccuaug cccacuucgu ggauacgcuc     2400

agcucuaaga aauuugaaga cuccauucug uucagcuacc aaguggauuc uaaguacaau     2460

cgcaaaauca gcgacgcuac aauuuaugcc accagaaagg ucaagcugga uaaagacaag     2520

uccgaggaaa cauacgugcu cgggaaaauc aaggauaucu auagccaggc uggcuacgac     2580

gccuuuauua aaaucuauaa caaggacaag ucuaaauucc ugauguacca uaaggauccg     2640

cagaccuuug agaaaguuau ugaggaaauc cuucggauuu auccuuccaa ggaguucaau     2700

gagaaaaaca aggaggugcc cugcaacccu uuugaaaagu accgccagga gaauggaccc     2760

aucuguaaau acagcaagaa aggcaacggg cccgagauua agucccugaa auauuacgac     2820

aagaauaucc cugaaucuuu caucgauauu accccgagca acuccaagaa uaaagucauc     2880

cucagacaac ugaaccccug gcggacagac guguauuuua aucaccagac ggagaaguac     2940

gaacugcuug gacucaaaua ugcggaucug caguucgaga agaaaaccga aacauacucu     3000

auuaccaucg acaaguauaa cgagauuaag gagaaagaag gcguugggga gcacagcgaa     3060

uuuaaguuca cacuguacaa aaaugaucuc cugcuuauca aggacaugga gaucaaagag     3120

gaacagauuu uucgcuuccu guccagaacc augccuaaca agaaacauua cguggagcuc     3180

aagcccuauu acaagggaaa uuucgaaggc aaagagcgga ucaaggucuu uaacacgaca     3240

ucuaauggga gaugccugaa aggccuggga aagaaaggcc uuagcauuua uaagaucaag     3300

accgaugugc uggggaacaa acacuucauu aagaaagaag gagagaagcc uaaacucaag     3360

uuuuga                                                                3366


<210>  3947
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3947
auggacagaa ccgaggugaa caucggcuuc gauauuggga ucagcuccgu cggauggucu       60

guuguggcca caaagagcgg ccggauucug gaauccgggg ugucucucuu uagcggagcu      120

uccgccgcga aaaaugagga acgcagaaac uuccggcagu cucgcagacu gcuucggcgc      180

agaaagaauc ggaucagcga ccugaaaaag uaccucaaag agcaauccuu uacguauaac      240

ggcgaacugc uuaaugagaa ccccuacgag auucgcguca aggggcugac cgaaaagcuc      300

agcaaagagg aaaucgcucu ggcccugcuc cacaucguga agagacgggg aguuucuuau      360

gaucugggcg acauugagga ggaaggcaca accggguccu acaaagaggg agugaagaaa      420

aaucagaaac uucugaagga aaagaccccu gccgagaucc aguuccagcg ccucaagaaa      480

uuucauaagg ucagaggcca aauugcuauc gaugaaaacg acgugcugcu uaauguuuuc      540

ccgaaccacg ccuaugugga ugaggcucag cagauucugu acacacagca agaguuuuau      600

cccgaaauca augagacauu cauuaaccag gucaccgacu uuaucagccg gaaacgcgau      660

uacuucaucg ggccuggauc ugaaaagucc agaacggacu acggcauuua ucggaccgau      720

gggacaaccc uccagaaucu guuuagcauc cugauuggaa aagacaagau cuuccccgau      780

gaguuuagag ccgcgggcaa cucuuacaca gcccaggugu auaaucuccu gaacgaccuu      840

aacaaucugg cuauugacga uaccgaaacg ggcaagcuca caaaacaaca gaaggagaaa      900

aucaucaugg agcugaagac cacagaaaaa uccgugaaca ugaugaaacu uauugccaag      960

accgcugggg ucaccguuga gcagaucagc cgcuaccgga uuaauagaga cggaaagucc     1020

gaaauccaua acauggccgu guaucgcaag auucggaaag uguuccugga gcucggcuac     1080

gaaaucaaug aguggccuuc ucaacugcug gaugagcucg gggacgcgcu gagccuuaac     1140

acagaaaaug gagagaucag acgcauucug acggauaacc ucgccaauaa guauaaaauc     1200

cuggaccagc agcuuauuaa ccugaucauu gaaaauaaga acgcuuucac ccucuccaca     1260

aaucagaagu ggcaccgguu uagcuacaaa accaugaaga uccugauucc cgagcugcuc     1320

gagacaaaca aagaacaaau gaccauccug acggagaugg gccuuaugaa gaaagaucag     1380

agaaauuacg ucgggaagaa caagaucgac ucugaagccc ugcucgagaa ccuguauaau     1440

ccugugguuc gcaaauccac acggcagacc augaagcugu ucaacacccu ucucaaaaag     1500

uacccgaaua uugcuuaugu ggucaucgaa augcccagag aggaugacga ggauaaagcc     1560

cgccgggacu acgugaaguu ucagaaagac aacgaaaagg agaaggaugc cgcucugaaa     1620

aauuucauua uggaaagcgg aaagucugag gcggaaguua ucgccaaaua uagacaaagc     1680

aagaaauuuc gguccaaggu gcgccugugg uaccagcaga ugggcagaug ccucuauucu     1740

ggcaaggcua ugccugccga gcgggucuuc agagacgauc auuuuacaga cauugaucac     1800

gugauccccc ugagcgucuc cuucgacgau gggaugaaca auaaagugcu uugucuggcc     1860

gagaugaacc gccagaagga caaacaaacc ccuuacgccu uuuucaaugc uggacacggc     1920

cagggguuuc aggcgaugaa cgcuaugcuc aagaauaaca aucggaucag cucugccaaa     1980

aagagaaacc ugcuguucac agaaaaucuu aacgaucucg agguucgcaa gagauuuauu     2040

gaacggaacc ugguggacac gcgcuacgcc uccagagucg ugcugaauga gcuccagcaa     2100

uucguuaaag cuaagcagcu ggauaccaaa gugacaguca uccggggaaa guucaccagc     2160

aaacuucgcg aaaaguggaa cauuaauaag ucuagagaca cacaucacca ucacgccgug     2220

gaugcugccg ugaucgcggu uucccccaug cugaaccucu gggagaaagg cgccagcauu     2280

aucccgaaua aggucgggga gaacgugauc gacauuaaaa ccggcgaaau cauuacggac     2340

cacgauuaug aggcugacgu guacgaacug ccuuaugcca aguuucugga gcagaucccc     2400

aaagucaaga aagauauuaa guuccggcau caaguggaca agaaaaagaa ucgcaaaguu     2460

uccgaugcua ccaucuacac aaccagaaag gccaaagugg gaaaggacaa ggaggaaaca     2520

gauuaugucc uuggcaccau caaagacauu uacucucucg agggguaugu gagcuuuaag     2580

aaaaucuaca acaaggauaa agcgaaguuc cugauggccc ggauugaccc uaagacguuu     2640

gaaaaacugg agaagaucgu ugcugaauac cccgagucca uugagguggu ccaggauaau     2700

ggaaaagugg acguucagaa gaucucuccc uucgaacucu aucgcaaaga uaacggcuuu     2760

gugacaaagu ucgccaagaa aaauaacggg ccugcuguca agagccugaa auacuaugac     2820

uccaaacuug gaagcucuau ugacgugacc ccggauaaug ccaagaacaa gaaggugauc     2880

cugcaguccc ucaaacccug gagaacagac gucuacuuua augaggaaaa ccaagaguau     2940

gaaaucaugg gccugaagua cagcgaucug aaauucggga agaacggcaa auauggaguu     3000

ccuaaggugc gguacgagga gauuaaacag gaagaggaaa ucucugaggg cuccaaguuu     3060

cuuuucagcc ucuacagagg ggaccgcauu aaggugacca augaacuggg agagaaccug     3120

gaucuccugu uuucuagccg gacccuuccc gcccagaaag gcuaugucga gcugaagccu     3180

aucgacaaaa caaaguucga ugggaaagaa aagguguccu ucuacggaac gguuaccccc     3240

aauggccagu uugugaagaa auucgcuaag aaaggguaua agauucucaa acugaacaca     3300

gacgagcugg gcaauaccua caucgaaaac ggagagaagg agccgagcga uauccuugac     3360

auguga                                                                3366


<210>  3948
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3948
augaacggcc uggugcucgg gcuggacauc ggaauuggca gcgucggggu uggaauccuu       60

aauaaggaua ccggcgagau uauccacgcc aacuccagaa uuuuccccgc ugccacagcg      120

gacaauaacg uggugcggcg cucuagcaga caggggcggc gccugaauca ucggaaaaag      180

cacagauccg uccgccucaa agaucuguuu gaauacuaug accuucugac ggauuucucu      240

aaggugagca ucaaccucaa uccuuacaga cugcggguua acggacugaa ucaacagcuc      300

accaacgagg aacuguuuau cgcucuuaaa aauauuguga agcgcagagg caucuccuau      360

cuggacgaug ccucugagga cggcggggcc gucagcuccg auuacggaaa ggcuguggaa      420

gagaaccgga aacuccuggc cgagcagaca ccgggccaga uucaacuuga acgcuucgag      480

aaguaugggc agguuagagg agacuuuacc guggucgaaa auggcgagaa acggcgccug      540

aucaacgugu ucagcacauc ugcuuacaag aaagaggccg aacggauucu cagaaagcag      600

caggaguaua auaaccaaau cauggaugaa uuuauugcgg acuaccugac cauccugaca      660

gggaagagaa aauacuauca uggacccggc aacgagaagu cccgcaccga uuacggccgg      720

uucagaacgg acgggaccac acucgacaau aucuuuggca uucugaucgg aaaaugcacc      780

uucuauccug aaaaguaccg cgccagcaaa gccucuuaua cagcucagga guuuaaccuu      840

cugaaugauc ucaacaaucu gaccgugccc acggagacaa agaaacuuuc cgaagaacag      900

aagaagacca uugucgaaua cgcuaaaagc gccaagacac ugggggcguc uacccuccug      960

aaauauaucg ccaagaugau ugacgcuucc guugaucaga uccggggaua cagaguggac     1020

gugaaaaaca agccugagau gcacaccuuc gaagucuacc gcaagaugca aagccuggag     1080

acaaucuccg ugggcgaguu uucucgggaa guucucgaug agcuggccca ugugcuuacg     1140

cugaauaccg aaagagaggg gauugaagag gcuaucaaca caaaacuccg cgacagcuuc     1200

ucccaggauc agauucugga gcuuguccaa uuccggaaga auaacagcuc ucuguuuucc     1260

aaaggauggc acaauuucag ccucaagcug augauggaac ugauccccga gcucuaugaa     1320

accucugagg agcagauggc cauucugaca agacuuggca aacagaaguu uaaggaaacc     1380

agcaaacgca cgaaguacau cgacgagcag gaagugacag aggaaaucua uaacccuguu     1440

guggccaaau ccguccggca agcuauuaag aucauuaaug aggcgaccaa aaaguacggc     1500

aucuucgaua acauugugau cgagauggcc agagaaaaua acgaggaaga ggcuaagaaa     1560

gacuauauua agcggcagaa agccaaccac gaugaaaaga augccgcuau ggagaaagcc     1620

gcguuucagu acaacgggga gaaggaacug ccggacucua ucuuccaugg acacaaagag     1680

cucgcuacca agguccgccu guggcaucag caaggcgaaa agugucugua uacagggaaa     1740

aauaucccca uuagcgaccu uauccacaac caguacaagu acgagauuga ucacauccuc     1800

ccucuguccc ugagcuuuga cgauucucuc uccaauaaag ugcugguucu ugccaccgcc     1860

aaccaggaaa agggacagag aacacccuuc caagcucugg acagcaugga ugacgccugg     1920

ucuuaucggg aguuuaaauc cuacgugaag gauagcaagu cccucggcaa uaaaaagaaa     1980

gacuaucugc ugacggagga agauauuucu aagaucgagg ucaaacagaa guucaucgaa     2040

agaaaccuug uggacacccg cuacagcucc cggguugugc ucaauacacu gcaggauuuu     2100

uauaaggcuc auaaauucga caccacaauu ucugucguga gagggcaguu uaccagccaa     2160

cugcgcagaa aguggggccu cgagaaaucc cgggagacgu accaccauca cgccguggau     2220

gcgcugauca uugccgcuag cucucagcuu cgccugugga agaaacagaa caauccucuc     2280

aucuccuaua aggaaggaca auucguugac agcgagaccg gcgaaauuau cucucugaca     2340

gacgaugagu acaaggaacu ggucuucaaa gcccccuacg accauuuugu ggauacccuu     2400

agauccaaga aauucgagga cagcauccuc uuuucuuauc agguggauag caaguucaac     2460

cggaaaauuu ccgacgcuac aaucuacgcc acccgcaaga cgaaacugga uaaggacaag     2520

agcgaggaaa cauauguccu ggggaaaauu aaggauaucu acucucaguc cggauaugac     2580

gcguuuauua aaaucuacaa uaaggauaaa agcaaguucc ucauguauca caaggacccg     2640

cagaccuuug agaaagugau cgaagagauu cugagaacau acccuuccaa ggaguucaac     2700

gagaaaaaua aggagguuuc uugcaacccc uuugaaaaau acaagcaaga gaacggcccu     2760

auccggaagu auagcaaaaa ggggaaugga cccgaaauua aaucccuuaa guacuaugau     2820

aaaaacaucc ccgagucuuu cauuaauauc accccuagca acuccaagaa uaaggugauu     2880

cugcgccagc ucaacccgug gagaaccgac gucuacuuua aucaccagac agaaaaauau     2940

gagcugcugg gccuuaagua cgccgaccuc caguucgaga aaaagacggg gaccuauucu     3000

aucacaaucg auaaauacaa cgaaauuaag gagaaggaag gcgugagcga gcauuccgag     3060

uuuaaauuca cccuguacaa gaaugaccug cuccugauca aagauacaga aauuaaggag     3120

gaacaaaucu uccgguuucu uagccgcacc augcccaacc agaaacacua uguugagcug     3180

aagccuuacu auaaaggaaa uuucgaaggc aaggagagaa uuaagguguu uaacacgaca     3240

ucuaaugggc ggugucucaa aggacugaga aagaaaggcc uguccaucua caagaucaaa     3300

accgacgucc uugggaacaa gcauuucauu aagaaagagg gagaaaagcc caaacugaag     3360

uuuuga                                                                3366


<210>  3949
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3949
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggugcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcaucg ucagacugaa ucggcuuuuu gaggaaagcg ggcugaucac cgacuucaca      240

aagauuucca ucaaccucaa uccuuaccag cugcgcguga aaggacugac cgaugagcuc      300

ucuaacgaag aacuguuuau ugcgcuuaag aauaugguua agcacagagg caucagcuau      360

cuggacgaug cuuccgacga uggcaacucu agcguggggg acuacgccca gauugucaaa      420

gagaauagca agcagcucga aacaaaaacc ccgggacaaa uccagcugga gcgguaucag      480

acauacggcc agcuucgcgg ggauuucacc guggaaaagg acggaaagaa gcauagacug      540

auuaacguuu uucccacgag cgccuaucgg ucugaggcuc uccgcauccu gcaaacccag      600

caggaguuca auccucagau cacagaugaa uuuauuaaca gauaccugga gauccucacc      660

ggcaagcgga aauauuacca cgggcccgga aaugaaaagu ccagaacaga cuacggccgc      720

uaucggacca gcggcgagac gcuggauaac auuuucggga uccuuauugg aaaaugcaca      780

uuuuacccug acgaguucag agccgcuaag gccucuuaua ccgcgcaaga guuuaaccug      840

cucaaugacc ugaacaaucu uacagugccc accgagacca aaaagcuguc caaagaacag      900

aagaaccaga ucaucaauua cgucaagaac gagaaagcca ugggcccugc uaaacucuuc      960

aaauauauug ccaagcugcu gagcugugau guggcugaca ucaaagaaua ccgcauugau     1020

aagucuggga aggccgagau ccauacauuu gaagcguauc ggaaaaugaa gacgcucgag     1080

acccuggaca uugagcaaau ggauagagaa acacuugaca aacuggccua cgugcucacc     1140

cugaauacag agcgcgaagg aauccaggag gcucuugaac acgaguucgc cgauggcucc     1200

uucagccaga agcaggucga cgagcugguu caauuucgga aagcuaacuc cucuaucuuc     1260

gggaagggau ggcacaauuu uagcgugaag cucaugaugg aacugauucc ggagcuguac     1320

gaaaccuccg aggagcagau gacgauccuc acaagacugg gcaaacagaa gaccaccucc     1380

ucuuccaaca aaacaaagua uauugaugaa aaacuucuga ccgaggaaau cuacaauccc     1440

guggucgcca agagcgugcg ccaggccauu aagaucguua acgcugcgau uaaagaguau     1500

ggcgacuucg auaauaucgu gaucgaaaug gcccgggaga caaacgagga cgacgaaaag     1560

aaagcuauuc aaaagaucca gaaagccaau aaggaugaga aagacgccgc uaugcucaag     1620

gccgcgaacc aguacaacgg gaaggcugaa cugccucauu cugucuuuca cggacauaaa     1680

cagcuggcca cgaagauuag acuuuggcac caacagggcg agcgcugccu cuauaccggg     1740

aaaacaauca gcauucacga ucugaucaau aacuccaauc aguucgaagu ggaccauauc     1800

cugccccucu cuauuaccuu ugaugacagc cuggccaaca agguucuugu guacgcuaca     1860

gccaaucagg agaaaggaca acggaccccu uaucaggcuc uggauuccau ggacgaugcc     1920

uggagcuuca gagagcucaa ggcguuuguc cgggaaucua agacgcuguc caacaaaaag     1980

aaagaguacc ugcuuaccga agaggacauc agcaaguucg augugcgcaa aaaguuuauu     2040

gaaagaaauc ucgucgacac acgguacgcc ucuagagugg uucugaacgc ucugcaggag     2100

cacuuccgcg cccauaagau cgauaccaaa guguccgucg ugcggggcca auuuacaagc     2160

cagcucagac gccacugggg gauugagaag accagagaca cguaucauca ccacgcuguu     2220

gacgcccuga ucaucgcggc cuccauucag cuuaaucugu ggaaaaagca gaaaaacaca     2280

cucgugucuu acagcgaaga ucaacugcug gacaucgaga ccggcgaacu uauuuccgau     2340

gacgaguaua aggagucugu cuucaaggcu cccuaccagc auuucgugga uacacucaaa     2400

agcaaggaau uugaggacuc cauccuguuc agcuaucagg uggauucuaa auuuaaucgg     2460

aagauuuccg acgccaccau cuacgcuacc cgccaggcca aaguuggaaa ggauaaagcc     2520

gacgaaacau auguccuggg caagaucaag gauauuuaca cgcaagacgg guacgaugcu     2580

uucaugaaaa ucuauaagaa agacaagagc aaauuucuca uguacagaca cgacccgcag     2640

accuucgaga aggugauuga accuauccug gagaacuauc ccaauaagca gauuaacgag     2700

aaaggaaagg aagugccugu caaucccuuu cuuaaauaca aggaggaaca uggcuauauc     2760

cggaaauacu cuaagaaggg gaacggaccc gagauuaaau cccugaagua uuacgauagc     2820

aaacucggca accacaucga caucacaccu aaggauucua auaacaaagu gguucugcag     2880

agcguguccc cguggcgcgc ggacgucuac uucaauaaga ccacagggaa guaugaaauu     2940

cugggccuua aauacgccga ucuccaauuu gagaagggaa ccggcacgua uaaaaucagc     3000

caggagaagu acaacgacau uaaaaagaag gaaggggugg auucugacuc cgaguucaaa     3060

uuuacacugu auaagaauga ucugcuccug guuaaagaca ccgaaaccaa ggagcagcaa     3120

cuuuucagau uucugagccg gacaaugccc aaacagaagc acuacgugga acucaaaccu     3180

uaugauaagc agaaguucga gggaggcgag gcucugauca aaguccuggg gaacguggcc     3240

aauuccggac aguguaagaa aggccuuggg aagucuaaca uuagcaucua caaaguucgc     3300

accgacgugc ugggcaauca acauaucauu aagaacgaag gagacaagcc caaacucgau     3360

uucuga                                                                3366


<210>  3950
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3950
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaaaauu ccggaaaaau uauccacgcu aacucuagac uguuccccgc cgcgaccgcu      120

gauaauaacg uggaacggcg caagaauaga caggcccggc gccucaacag acggaaaaag      180

caucgcggcg ugagauuuca agaccuguuc gaggauuacg ggcuucugac agacuuuagc      240

aaagucucca uuaaucucaa cccuuaucgg cugcgcgugg auggacugaa ucagcagcuc      300

acgaacgaag aacuguucau cgcccuuaag aauaucguua agagacgggg cauuucuuac      360

cuggacgaug cuagcgaaga cggcggggcc guguccucug auuauggaaa agcugucgag      420

gagaaccgca agcuccuggc cgaacagacc ccgggccaaa uccagcuuga gagauuugaa      480

aaauacggac aggugcgggg agacuucaca guuguggaga auggcgagaa gugccgccug      540

auuaacgucu uuagcaccuc cgcguauaga aaagaagccg agaagauccu ccggaagcag      600

caagaguuca auaaccagau uacagaugag uuuaucgaag acuaccugaa aauucugacc      660

gggaagagaa aauauuacca cggacccggc aacgaaaaga gccgcacaga uuacggccgg      720

uucagaaccg acgggacgac ccucgacaau aucuuuggag ugcugaucgg caaauguaca      780

uucuauccua acgaguaccg cgcuucuaag uccagcuaua ccgcccagga auuuaaucuu      840

cugaacgauc ucaauaaccu gacagugccc accgagacga aaaagcuguc ugaagaacag      900

aagaaaguca uuguugaaua cgcuaaggag gccaaaacac uuggggcguc cacccuccug      960

aaguauaucg ugaaaaugau ugacgccagc guggaucaaa ucucuggaua ccgggucgac     1020

gugaauaaca agccugagau gcauacauuc gaaguuuaua gaaagaugca gucccuggag     1080

accauuagcg ugggcgaacu cucccgcaau guccuggaug agcuugcuca cauccugacc     1140

cucaacacag aacgggaggg gaucgaggaa gccauuaauu cuaaacugau gggaguguuc     1200

agccaggacc aaguucuuga gcuggugcag uuuagaaaga acaauuccag ccucuucucu     1260

aaaggcuggc auaacuuuuc ccugaagcug augauggaac ucauccccga gcuguacgag     1320

acgagcgagg agcagaugac cauucuuaca cgccugggca aacagaaguc uaaggaaacc     1380

agcaaacgga caaaguacau cgaugagaaa gaacucaccg aggagauuua uaauccuguc     1440

guggcuaagu ccgucagaca agccaucaaa aucauuaacg aagccacgaa gaagcacggg     1500

aucuucgaca acauugugau cgagauggcu cgcgaaaaua acgaggaaga ugcgaaaaag     1560

gacuacauua aacggcagaa ggccaaucag gaugagaaaa acgcugccau ggagaaggcc     1620

gcuuuucagu auaauggaaa gaaggaacug ccgggcaaca ucuucaaugg gcacaaggag     1680

cuggccacaa aaauuagacu uuggcaucaa cagggagaaa agugccucua caccggcaaa     1740

accaucccca ucucugaccu gauucacaac caguauaagu acgagaucga ccauauucug     1800

ccucucagcc uguccuuuga ugacagccuu ucuaauaaag uucuggugca cgcgacagcu     1860

aaccaggaaa aggggcaacg gacccccuuc caggcccucg auuccaugga cgaugccugg     1920

agcuaucgcg aguuuaaguc uuacgucaaa gaccugaagu cccugagcaa uaaaaagaaa     1980

gauuaccuuc ucacagagga agacaucucc aagauugagg ugaagcagaa auucaucgaa     2040

agaaaccugg uugauacgcg guauucuagc agaguggucc ugaaugcucu ccaggacuuu     2100

uacaaguccc accaauucga uaccacaauc ucuguggugc gcggccaguu uaccagccag     2160

cugcggagaa aauggggacu ugagaagucc cgcgagacau aucaucacca ugccguugac     2220

gcucugauua ucgccgcgag cucucaacuc agacugugga aaaagcagaa caauccucug     2280

auuaccuaca aggaaggcca guucgucgau ucccagacgg gggagaucau uagccuuacc     2340

gacgacgaau auaaagagcu cguguucaag gcccccuacg aucacuuugu ggacacacug     2400

cauucuaaaa ccuucgaaga uggaauccug uuuuccuauc aagucgacag caaguacaac     2460

cggaaaaucu cugaugcuac aauuuacgcc acccgcaagg cuaaacucga caaggauaag     2520

agcgaggaga cguaugugcu gggcaaaauc aaggacauuu acucccaggc cggguaugau     2580

gcguucauca aaauuuacaa caaggacaaa agaaaguuuc uuauguauca caaggauccg     2640

cagacauucg aaaaaguuau cgaggaaauc cugcggaccu acccuagcaa ggagcgcaau     2700

gacaaaaaca aggaagugcc cuguaauccu uuugagaaau auagacagga gaacggaccc     2760

auucggaagu acucuaagaa aggcaauggg cccgaaauca agugccucaa auacuaugau     2820

aacaagcugg gcaauuacau ugacaucucc ccugacggaa gcgauaacca agucgugcug     2880

cagucccuua aaccguggcg cacagacguu uauuucaauc accagaccaa ggaguacgaa     2940

cucaugggcc ugaaguauuc ugaucugagc uuugagaaag gguccggaaa guacucuauu     3000

agcaacgaaa aauauaauuc caucaagaga auugagggcg ugaacgagca gucugaguuc     3060

aaauuuaccc ucuacaagaa ugaccugauc cuuaucaagg auagcgagaa caaugaacaa     3120

aaacuguucc gguuuaacuc ccgcaacgac acagccaagc auuacgucga gcucaaaccc     3180

uaugauaagg cuagauucga cgggcagcag gagcugauua cgauccuggg aaauguggcc     3240

aaaggcgggc aaugucuuaa gggccugaac aaaagcaaua uuucuaucuu caagguuaag     3300

accgaugugc ucgggaaaaa gcacauuauc aaaaaggaag gcgacgagcc uaaacugaag     3360

uuuuga                                                                3366


<210>  3951
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3951
auggccgaca agacccuggg cgcucucaca uucggguuug auaucggaau ugccagcgug       60

ggcuggugcg uccuggggga gaacagaauc guugaccuug gagugcgggc guucgauaaa      120

gcugaaacgg ccaaggaggg cgaaucccug aaucucgccc gcagaaccgc ucggcugaug      180

cgcagacggc uucgcagacg ggccuggcgc cugacaaaac ucgcuagacu gcugaagcgg      240

gaggggcuca uugccgacgc guuucagaug caccaacccu cugccaccag cccuuggaaa      300

cugcgcgugg aagcucuuga uagacggcug acagacgaug agugggcccg cgucaucuac      360

caucucugua agcacagagg auuccauugg auuucccggg cugaggaaaa gaaagccgag      420

ggcgacgcga agggcgaauc ugggagagug aaaaagggac ugagcgauac ccagcgccgg      480

augggcgaga aaggguauag auccgccgcu gagaugguuc uugccgaauu uccggaggcu      540

cagcgcaaca agcagggaga cuacggcaag gcccugucuc gggugcuccu gagcgaagaa      600

cuggcccucc uguucagacg ccaacgggaa cuugggcaca cacaugcuuc caaagagcug      660

gaggcggcca uccucggaaa uggcgauaga aagggcgggc uguuuugggc ucagaaaccc      720

ccucuugccg gagccgaccu gcucaagaug cugggcaaau gcaccuucga aaaggaugag      780

uaucgcgcuc ccaaagccag cuuuacggcg gaacggcacg ucuggcugac cagacucaac      840

aaucugcgcg ugguugugga cgggacaacc cggccucuua cagaggacga aagacggguc      900

gcucugcccc ucccuuacgg auaugccgau gccaagcuga ccuacaagca gcuucgcgcu      960

gcccugucua aagcuggccu cuccaacuuc agcuuugccg ggaucgcgga cgauaaggag     1020

aaagacacgc ugacaaagcu gccggccugg gagacccucu cuaaaacacu gaagggaaag     1080

ggccuugaaa ccgaguggga aggcauggcu ggggccgcua ccggaggcga ucccacacug     1140

cucgacgaga uuuccagagu gcugagcgug uauaaagaug gggacgaagu cgauguugag     1200

cugcggaagc ucccucuccc cggaggcgag aaaauggugg aaacgcuguc ugagaucucc     1260

uucgacaagu uucacgcccu gagccucaaa gcgcugagaa agauugugcc ucauauggaa     1320

accgggcuuc gcuacgauga ggccugugcu gccgcuggcu accaccauuc ccagcugcac     1380

gaggccggag ccggcgggca aaaguaucuu cccccguucu acacacggag agacaaagac     1440

ggacgcaugg ucuucgauga ggagcuggac gugccuagaa aucccguugu gcugcgggcu     1500

cuuaaccagg cgcgcaaggu ccucaaugcc cuggugagag cuuauggcuc uccuagcgcc     1560

guucacaucg aaauggcccg ggaucugucc aaaccccucg uggggcaucc caccccuaac     1620

ggaggcuaca uugaggggcg cagagacauc gaaaagcugc agaaagagua ucgggagcgc     1680

aaugaaaagg auaaggcugc cuuugcggcu gagcacggca uugccgccaa aagcagagag     1740

uucgaaaagu uucagcuuua ccgggaacaa cagggaaaau gcgcuuauuc ucugaagccg     1800

cucgaccugc aucgcguccu gcacgauauc ggcuacgccg agguggacca ugcgcuuccc     1860

uacuccagaa gcuaugauga cgggaaaaac aauaaggucc ucgugcugac agaggaaaac     1920

cggaauaaag gaaacagaac cgcuuacgag uaucugugug ccuucccugg cggggaaaac     1980

ggagagcgcu ggcggaauuu uguugccuac guggaaucua acaagagcua uagacucgcu     2040

aagcgcacgc ggcugcuuag aaaagauuuc ggcgggaagg ccgcugagga guuucgcgaa     2100

cggaaucuga acgacacaag auacaucugc aaauucuuua agaauuucgu cgagcgcuuu     2160

cuccagcuga aagugggcgc cgauggaacc gcggccaagc gguguguugu gcuguccggc     2220

caaaccacag cuuuccuuag agcccggugg gggcuccuga agguccgcgg agcuucugac     2280

agacaccacg cccuggacgc cgcuguggug gcggccugca gccauggcau gguuaaacgg     2340

cucgcugauu auucccgcac caaggaacug gccaaaguca gagacggguu ucccgauccu     2400

gagacaggag agauugugga ccccgccaug uuccagcagc uucacgcuca uuucccggau     2460

ccuuggcccc acuuucgggg cgaacuggag gccagacucc agcuggacga uagcgcggaa     2520

cugcgcgcuc aacuugagau guucgggacg uacccugccg aagcccugga gacccuccgg     2580

ccccuguuug ugucuagagc uccucagcgc cggaacggcg gagccgcgca caaggagaca     2640

aucuacgcuc agcccgaaag acucaaagag cagggcgggg ucacccaaaa ggugucccug     2700

gccagccuua agccgacaga cauugauaaa cugaucgacc cucaucgcaa ugaaaagcuc     2760

uaugccgcua uucgggccag acuggaggcu cacggaggca aaucugauaa ggccuucccc     2820

gcggacaacc cccugcgcaa accugauaga gacgggaauc cgaccggacc caucguucgg     2880

gccgugacga uggucaucga caaacuuucc ggcauucaga uccgcggcgg gauugcuaag     2940

aacgauacca ugcugagagu ggacguuuuu cggcauaaga aggauggaaa auuccaccuc     3000

gugccucugu acguccauca cgccguggcu caggaacucc ccaaucgcgc caucguugcc     3060

uuuaaggacg aggaugagug gacacugauu gacgauaacu uccacuuucu uuucagcuuu     3120

cauccuaaug acuucaucag aaucacccag aaaaacaagg cucccauucu gggcuauuac     3180

gcguccuguc accgggggac aggaaaucuc aaccuguggg cccaugauag aaaccaaaaa     3240

gugggcaagg acgggcagau cgaaggaauu ggcgucaaga ccgcuguggc ccuggagaaa     3300

uuucacgugg auguccuugg caauaucuau ccugcucccc cggaacagcg ccaugggcug     3360

gccuga                                                                3366


<210>  3952
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3952
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggugcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcagag uccggcugaa ucgccuuuuu gaggaaagcg ggcugaucac cgacuucaca      240

aagaucucca uuaaccucaa uccuuaccag cugagaguga aaggacugac cgaugagcuc      300

ucuaacgagg agcuguuuau cgcgcuuaag aauaugguua agcaccgggg cauuagcuau      360

cuggacgaug cuuccgacga uggcaacucu agcguggggg acuacgccca gaucgucaaa      420

gaaaauucca agcagcucga aacaaaaacc ccgggacaaa uucagcugga gcgcuaucag      480

acauacggcc agcuuagagg ggauuucacc guggaaaagg acggaaagaa gcaucggcug      540

aucaacguuu uucccacgag cgccuaucgc ucugaggcuc uccggauucu gcaaacccag      600

caggaguuca auccucagau cacagaugaa uuuaucaaca gauaccugga gauucucacc      660

ggcaagagaa aauauuacca cgggcccgga aaugaaaagu cccgcacaga cuacggccgg      720

uauagaacca gcggcgagac gcuggauaac aucuucggga uucuuaucgg aaaaugcaca      780

uuuuacccug acgaguuccg cgccgcuaag gccucuuaua ccgcgcaaga auuuaaccug      840

cucaaugacc ugaacaaucu uacagugccc accgagacca aaaagcuguc caaagaacag      900

aagaaccaga uuaucaauua cgucaagaac gagaaagcca ugggcccugc uaaacucuuc      960

aaauauaucg ccaagcugcu gagcugugau guggcugaca uuaaagggua ccggaucgau     1020

aagucuggaa aggccgaaau ucauacauuu gaggcguaua gaaaaaugaa gacgcucgaa     1080

acccuggaca ucgagcaaau ggaucgcgag acacuugaca aacuggccua cgugcucacc     1140

cugaauacag aacgggaggg cauucaggaa gcucuugagc acgaguucgc cgaugggucc     1200

uucagccaga agcaggucga cgaacugguu caauuuagaa aagcuaacuc cucuaucuuc     1260

ggaaagggcu ggcacaauuu uagcgugaag cucaugaugg agcugauccc ggaacuguac     1320

gagaccuccg aggaacagau gacgauucuc acacgccugg gcaaacagaa gaccaccucu     1380

agcuccaaca aaacaaagua uaucgaugag aaacuucuga ccgaagagau uuacaauccc     1440

guggucgcca agagcgugcg gcaggccauc aagauuguua acgcugcgau caaagaauau     1500

ggggacuucg auaauauugu gaucgagaug gccagagaaa caaacgagga cgacgagaag     1560

aaagcuaucc aaaagauuca gaaagccaau aaggaugaaa aagacgccgc uaugcucaag     1620

gccgcgaacc aguacaacgg aaaggcugag cugccucauu cugucuuuca cggccauaaa     1680

cagcuggcca cgaagauccg ccuuuggcac caacaggggg aacggugccu cuauaccgga     1740

aaaacaauua gcauccacga ucugauuaau aacuccaauc aguucgaggu ggaccauauc     1800

cugccccucu cuaucaccuu ugaugacagc cuggccaaca agguucuugu guacgcuaca     1860

gccaaucagg aaaaaggcca aagaaccccu uaucaggcuc uggauuccau ggacgaugcc     1920

uggagcuucc gggagcucaa ggcguuuguc cgcgagucua agacgcuguc caacaaaaag     1980

aaagaauacc ugcuuaccga ggaagacauu agcaaguucg augugcggaa aaaguuuauc     2040

gagagaaauc ucgucgacac aagauacgcc ucucgcgugg uucugaacgc ucugcaggaa     2100

cacuuccggg cccauaagau ugauaccaaa guguccgucg ugagagggca auuuacaagc     2160

cagcuccgca gacacugggg caucgagaag acccgggaca cguaucauca ccacgcuguu     2220

gacgcccuga uuaucgcggc cuccucucag cuuaaucugu ggaaaaagca gaaaaacaca     2280

cucgugagcu acuccgagga ucaacugcug gacaucgaaa ccggagagcu uauuucugau     2340

gacgaauaua aggagagcgu cuucaaggcu cccuaccagc auuucgugga uacacucaaa     2400

uccaaggagu uugaagacag cauccuguuc ucuuaucagg uggauuccaa auuuaaucgc     2460

aagauuagcg acgccaccau cuacgcuacc agacaggcca aaguuggcaa ggauaaagcc     2520

gacgagacau auguccuggg gaagauuaag gauaucuaca cgcaagacgg auacgaugcu     2580

uucaugaaaa ucuauaagaa agacaagucu aaauuucuca uguaccggca cgacccgcag     2640

accuucgaaa aggugauuga gccuauccug gaaaacuauc ccaauaagca gauuaacgag     2700

aaaggcaagg aggugccuug uaaucccuuu cuuaaauaca aggaggaaca uggguauauc     2760

cgcaaauacu ccaagaaggg aaacggcccc gaaauuaaaa gccugaagua uuacgauucu     2820

aaacucggga accacaucga cauuacaccu aaggauagca auaacaaagu cgugcugcag     2880

uccguuagcc cguggagagc ggacguguac uucaauaaga ccacaggcaa guaugagauc     2940

cugggacuua aauacgccga ucuccaauuu gaaaagggca ccgggacgua uaaaaucucu     3000

caggagaagu acaacgacau uaaaaagaag gagggagucg auuccgacag cgaguucaaa     3060

uuuacacugu auaagaauga ucugcuccug gugaaagaca ccgaaaccaa ggaacagcaa     3120

cuuuuccggu uucugucccg cacaaugccc aaacagaagc acuacguuga gcucaaaccu     3180

uaugauaagc agaaguucga aggcggggag gcucugauca aagugcuggg aaacgucgcc     3240

aauucuggcc agugcaagaa agggcuuggc aagagcaaca uuuccaucua caaagugaga     3300

accgacguuc ugggaaauca acauauuauc aagaacgagg gcgacaagcc caaacucgau     3360

uucuga                                                                3366


<210>  3953
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3953
augaacggcc uggugcucgg gcuggacauc ggaauuggca gcgucggggu uggaauccuu       60

aauaaggaua ccggcgagau uauccacgcc aacuccagaa uuuuccccgc ugccacagcg      120

gacaauaacg uggugcggcg cucuagcaga caggggcggc gccugaauca ucggaaaaag      180

cacagauccg uccgccucaa agaucuguuu gaauacuaug accuucugac ggauuucucu      240

aaggugagca ucaaccucaa uccuuacaga cugcggguug acggacugaa ccaacagcuc      300

accaaugagg aacuguuuau cgcucuuaaa aacauuguga agcgcagagg caucuccuau      360

cuggaugacg ccucugagga uggcggggcc gucagcuccg acuacggaaa ggcuguggaa      420

gagaaucgga aacuccuggc cgagcagaca ccgggccaga uucaacuuga acgcuucgag      480

aaguaugggc agguuagagg agauuuuacc guggucgaaa acggcgagaa acggcgccug      540

aucaaugugu ucagcacauc ugcuuacaag aaagaggccg aacggauucu cagaaagcag      600

caggaguaua acaaucaaau cauggacgaa uuuauugcgg auuaccugac cauccugaca      660

gggaagagaa aauacuauca uggacccggc aacgagaagu cccgcaccga cuacggccgg      720

uucagaacgg acgggaccac acucgauaac aucuuuggca uucugaucgg aaaaugcacc      780

uucuauccug aagaguaccg cgccagcaag gcuucuuaua cagcccagga guuuaaucuu      840

cugaacgacc ucaauaaccu gaccgugccc acggaaacaa agaaacuuuc cgaggaacag      900

aaaaagacca uugucgagua cgcuaagagc gccaaaacac ugggggcguc uacccuccug      960

aaguauaucg ccaaaaugau ugaugcuucc guugaccaga uccggggaua cagaguggau     1020

gugaauaaca agccugaaau gcacaccuuc gaggucuacc gcaaaaugca acugcucgag     1080

acaauccccg ugggcgaauu uagccgggag guucuggacg aacuugccca uauucugacg     1140

cucaauaccg agagagaagg gaucgaggag gcuauuaaca caaagcugau ggauguguuc     1200

ucccaggacc agauccuuga acugguccaa uuccgcaaga auaacucuag ccucuuuucc     1260

aaaggauggc acaauuucag ccugaagcug augauugagc ucaucccuga acuguaugag     1320

accucugagg aacagauggc cauccuuaca cggcugggca aacagaaguu uaaagagacc     1380

uccaagagaa cgaaguacau ugaugaacag gaggugacag aagagaucua uaacccgguu     1440

guggccaaaa gcguccgcca agcuauuaag aucauuaaug aggcgaccaa aaaguacggc     1500

aucuucgaca acauugugau cgaaauggcc cgggagaaua acgaagagga agcuaaaaag     1560

gauuauauca agagacagaa agccaaccac gacgagaaga augccgcuau ggagaaagcc     1620

gcguuucagu acaacgggga aaaggagcuc cccgacucua uuuuccaugg acacaaagaa     1680

cuggcuacca agguccggcu guggcaucag caaggcgaga aaugucuuua uacagggaag     1740

aauaucccua uuagcgaucu cauccacaac caguacaagu acgaaauuga ccacauccug     1800

ccccuguccc ucucuuuuga ugacagccug uccaauaaag ugcuuguucu ggccaccgcc     1860

aaccaggaga agggacgcag aacaccuuuc caggcucucg auagcaugga cgaugccugg     1920

ucuuaucggg aguuuaaauc cuacgugaag gacagcaaau cucugggcaa uaagaagaaa     1980

gauuaucugc uuacggaaga ggacaucucc aagauugaag ucaaacaaaa guucaucgag     2040

agaaaccucg uggauacccg cuacagcucc cggguugugc ugaauacacu gcaggacuuu     2100

uauaaagcuc auaaguucga uaccacaauu ucugucguga gagggcaguu uaccagccag     2160

cuccgcagaa aguggggccu ggagaaaucc cgggaaacgu accaccauca cgccguggac     2220

gcgcuuauca uugccgcuuc uagccaacug cgccucugga agaaacagaa caauccccug     2280

aucuccuaua aggagggaca guucguugac agcgaaaccg gcgagaucau uucucugaca     2340

gaugacgaau acaaagagcu ugucuucaag gccccguacg aucauuuugu ggacacccuc     2400

agauccaaga aauucgagga uagcauccug uuuucuuauc aaguggacuc caaguucaac     2460

cggaaaauua gcgaugcuac aaucuacgcc acccgcaagg cgaaacugga caaggauaaa     2520

ucugaagaga cguauguccu cgggaagauu aaggacaucu acagccaguc cggauaugau     2580

gccuuuauca aaauuuacaa uaaggacaaa agcaaguucc ugauguauca caaagauccu     2640

cagacauuug aaaaggugau cgaggaaauu cuuagaaccu accccucuaa ggaguucaac     2700

gagaaaaaua aggaaguuuc cugcaacccu uuugagaaau acaagcagga aaacggcccc     2760

auccggaaau auagcaagaa ggggaaugga cccgagauua aaucccugaa guacuaugac     2820

aaaaacaucc cugaaucuuu cauuaauauc acaccgagca acuccaagaa uaaagugauc     2880

cuccgccaac ugaaccccug gagaaccgac gucuacuuua aucaccagac cgagaaguau     2940

gagcugcuug gccucaagua cgcugaucug caguucgaga aaaagacagg gacguauucu     3000

auuaccaucg acaaauacaa cgagauuaag gaaaaagagg gcgugagcga gcauuccgaa     3060

uuuaaguuca cacuguacaa gaaugaucuc cugcuuauca aagacaccga gauuaaggaa     3120

gaacagaucu uccgguuucu gucucgcaca augccuaacc aaaaacacua uguugaacuc     3180

aagcccuacu auaaaggaaa uuucgagggc aaggagagaa ucaaaguguu uaacaccacg     3240

agcaaugggc ggugucugaa gggacugaga aagaaaggcc uuuccauuua caagaucaaa     3300

acagaugucc uggggaacaa gcauuucauu aaaaaggaag gagagaagcc uaaacucaag     3360

uuuuga                                                                3366


<210>  3954
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3954
augaacggca agauccuggg gcucgacauu ggaguggcca gcgucggcgu ugggauccug       60

gauaaaaaga ccggagaaau uauccacgcu uccucuagaa uuuuccccgc cgcgacagcu      120

gacagcaaug uggaacggcg cggcuuuaga caggggcggc gccuuggaag acggaaaaag      180

caucgcaaag ugagacuggc cgaucucuuc uccgacacgg gccugaucac cgauuuuucu      240

aaggucagca ucaaccuuaa uccuuacgag cugcggauua agggccucaa cgaaaaacug      300

acaaaugagg aacuguucau cgcccucaag aacauuguga aacgcagagg gaucuccuau      360

cuggacgaug cuaaugagga cggagagucu agcuccagcg aauacggcaa ggccguugag      420

gaaaaccgga aacuucuggc ugauaagacc ccggggcaaa uucagcucga gcgcuuugag      480

aaguauggac aggugagagg cgacuucaca aucgaagaga auggggaaaa acaccggcug      540

cuuaacgucu uuucuaccuc cgccuacaag aaagaggcgg aacgcauucu gacaaagcag      600

caagauuaua aucaggacau caccgaugag uucauccagg ccuaccucac gauucugacc      660

ggaaaaagaa aguauuacca uggccccggc aacgagaaaa gccggacaga cuacgggaga      720

uuucgcaccg acggaacaac ccuggauaau aucuucggca uucucaucgg gaagugcacg      780

uuuuauccug aagaguaccg ggcugccaag gcuucuuaua cagcccagga guucaaccug      840

cuuaaugacc ugaacaaccu caccgugccc acagagacca aaaagcuguc cgaagaacaa      900

aagagacaga uuaucgagua cgcgaaggga gccaaaaccc uuggcgcugc cacacugcuc      960

aaguauaucg cuaagcuggu ugaugggagc guggaagaca uuaaaggaua ccgcaucgau     1020

aagucugaga aaccugaaau gcacacguuu gacauuuauc ggaagaugca gacccuggag     1080

acagucgaug uggaaaaacu cuccagagag gugcuggacg agcuugccca uauccugacc     1140

cucaauacag aacgcgaggg cauugaagag gccaucaagg ucagcuucau caagcgggag     1200

uucgaacagg aucaaauugc ugagcugguu uccuuuagaa aaucuaacag cucccuuuuc     1260

ggcaaggggu ggcacaauuu uagcaucaaa cugaugaccg aacucauucc cgagcuguac     1320

gaaacgucug aggagcagau gacaauccug acccgccucg gaaagcagaa aaccaaggcg     1380

cgguccaaga gaacaaaaua cauugacgaa aaggagcuga ccgaugaaau cuauaacccu     1440

gugguggcca aaagcguccg ccaagcuauu aagaucauca aucuugccac gaaaaaguac     1500

ggcguguucg acaacauugu uaucgagaug gcccgggaaa auaacgagga ggacgcuaaa     1560

aaggauuaug ugaagagaca gaaagccaau gaagacgaga agaacgcggc uauggaaaaa     1620

gccgcccacc aguacaaugg gaagaaagag cugccggaua acgucuuuca uggacacaag     1680

gaacucgcua caaagauucg gcuguggcau cagcaaggcg agaaaugucu guauaccggg     1740

aagaauaucc ccauuucuga ccuuauccac aaccaguaca aauaugagau cgaucacauu     1800

cucccucuga gccuguccuu cgacgauucu cucgccaaua aggugcuggu ucuugcuaca     1860

gccaaccagg aaaaaggcca gcgcaccccc uuucaagcgc uggacagcau ggaugacgcc     1920

ugguccuaca gagaguucaa ggcuuacgug cggggagcca gagcucucag caacaagaaa     1980

aaggauuauc ugcugaauga agaggacauc aacaaaauug aggucaagca gaaauuuauc     2040

gaacgcaauc uuguggauac acgguacucu uccagagucg ugcucaacgc ccugcaggac     2100

uucuauaagc ugaaugacuu ugauaccaag auuagcguug ugcgcggcca guucacgucu     2160

caacucagac ggaaauggcg caucgacaag uccagagaga ccuaccauca ccaugcgguc     2220

gaugcccuga ucauugcugc cagcucccag cuucggcugu ggaaaaagca ggggaacccu     2280

cucaucucuu auaaagaaaa ucaguuugug gacagcgaga caggagaaau uaucucccug     2340

accgaugacg aguacaagga gcugguuuuc cgcgcucccu augaucacuu cguggacaca     2400

gucucuagca aaaaguuuga agaucggauu cuuuucuccu accaagugga cagcaaguac     2460

aacagaaaaa ucucugaugc caccaucuau uccacgcgca aggccaaacu cggcaaggac     2520

aaaagcgagg aaacauacgu gcuggggaag auuaaggaua ucuauaccca gacaggauac     2580

gacgcuuuua uuaaacugua uaagaaagac aagucuaaau uccucaugua ccauaaggau     2640

ccgaucaccu uugagaaggu uauugaagag auccugaaaa ccuauccuga caaggagauu     2700

aaugaaaaag gcaaggaggu cgcgugcaac cccuucgaaa aauacagaca ggagaauggg     2760

ccucuucgga aguacuccaa gaaaggcaag ggacccgaaa ucaaaagccu gaaguauuac     2820

gauaacaaac ucggcaauca caucgacauu acacccgaua acucugagaa ucaagugauc     2880

cugcagagcc ugaagccuug gcgcacggac guguauuuua accacaagac caaaauuuac     2940

gagcuuaugg ggcucaagua uuccgaucug agcuucgaaa aaggaucugg caaguacaga     3000

aucucccugg acaaauauaa cgucauuaag aaaaaggagg gggugcauaa ggaaagcgag     3060

uuuaaauuca cacucuacaa gaaugaucug auccuuauca aagaccugga gaaguccgaa     3120

cagcagcucu uucgguacaa cucuagaaau gauaccagca aacacuaugu ugagcugaag     3180

ccguacgaca aggccaaauu cgaaggaaac caaccccuga uggcucuuuu uggcaaugug     3240

gccaaagggg gccagugucu gaaaggacuc aacaaggcca auauuuccau cuauaaaguc     3300

cagacagacg ugcugggcaa caagcgcuuc auuaagaaag agggggaugc uccuaagcuc     3360

gaguucuga                                                             3369


<210>  3955
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3955
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggugcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcaucg ucagacugaa ucggcuuuuu gaggaaagcg ggcugaucac cgacuucaca      240

aagauuucca ucaaccucaa uccuuaccag cugcgcguga aaggacugac cgaugagcuc      300

ucuaacgagg agcuguuuau ugcgcuuaag aauaugguua agcacagagg caucagcuau      360

cuggacgaug cuuccgacga uggcaacucu agcguggggg acuacgccca gauugucaaa      420

gaaaauucca agcagcucga aacaaaaacc ccgggacaaa uccagcugga gcgguaucag      480

acauacggcc agcuucgcgg ggauuucacc guggaaaagg acggaaagaa gcauagacug      540

auuaacguuu uucccacgag cgccuaucgg ucugaggcuc uccgcauccu gcaaacccag      600

caggaguuca auucccagau cacagaugaa uuuauuaaca gauaccugga gauccucacc      660

ggcaagcgga aauauuacca cgggccugga aaugaaaaga gcagaacaga cuacggccgc      720

uaucggacca acggcgagac gcuggauaac auuuucggga uccuuauugg aaaaugcaca      780

uuuuaccccg acgaguucag agccgcuaag gccucuuaua ccgcgcaaga auuuaaucug      840

cucaacgacc ugaauaaccu uacagugccu accgagacca aaaagcuguc caaagaacag      900

aagaaucaga ucaucaacua cgucaagaau gagaaaguga ugggccccgc caaacucuuc      960

aaauauauug cuaagcugcu gagcugugau guggccgaca ucaaagggca ucgcauugau     1020

aagucuggaa aggcugaaau ccacacauuu gaggccuacc ggaaaaugaa gacgcucgaa     1080

acccuggaca uugagcaaau ggauagagag acacuugaca aacuggcgua uguccucacc     1140

cugaacacag aacgcgaggg cauccaggaa gcccuugagc acgaguucgc ugaugggucc     1200

uucagccaga agcagguuga cgaacuggug caauuucgga aagccaauuc cucuaucuuc     1260

ggaaagggcu ggcauaacuu uagcgugaag cucaugaugg agcugauucc ugaacuguac     1320

gagaccuccg aggaacagau gacgauccuc acaagacugg gcaaacagaa gaccaccucu     1380

agcuccaaua aaacaaagua cauugaugag aaacuucuga ccgaagagau cuauaacccg     1440

gucguggcua agagcguucg ccaggccauu aagaucguga augccgcuau uaaagaauac     1500

ggggacuucg auaacaucgu caucgagaug gcgcgggaaa caaaugagga cgacgagaag     1560

aaagccauuc aaaagaucca gaaagcuaac aaggaugaaa aagacgccgc caugcucaag     1620

gcugccaacc aguauaaugg aaaggcggag cugccccacu cuguguuuca uggccacaaa     1680

cagcuggcua cgaagauuag acuuuggcac caacaggggg aacgcugccu cuacaccgga     1740

aaaacaauca gcauucauga ucugaucaac aauccuaacc aguucgaggu ugaccacauc     1800

cugccccucu ccauuaccuu ugaugacucu cuggccaaua aggugcuugu cuaugccaca     1860

gcuaaccagg aaaaaggcca acggaccccu uaccaggccc uggauagcau ggacgaugcu     1920

ugguccuuca gagagcucaa ggccuuugug cgggagagca agacgcuguc uaauaaaaag     1980

aaagaauauc ugcuuaccga ggaagacauc uccaaguucg auguccgcaa aaaguuuauu     2040

gagagaaacc ucguggacac acgguacgcg agcagaguug ugcugaaugc ccugcaggaa     2100

cauuuccgcg cucacaagau cgauaccaaa gucucugugg uucgggggca auuuacaucc     2160

cagcucagac gccauugggg cauugagaag accagagaca cguaccacca ccaugccgug     2220

gacgcucuga ucaucgccgc gagcucccag cuuaaccugu ggaaaaagca gaaaaauaca     2280

cucgucucuu auagcgagga acaacugcug gauauugaga ccggagaacu uaucuccgac     2340

gaugaguaca aggagucugu guucaaggcc cccuaucagc acuucgugga cacacucaaa     2400

agcaaggaau uugaggauuc cauucuguuc agcuaccagg uugacucuaa auuuaaccgg     2460

aagaucuccg augcuaccau uuaugccacc cgccaggcua aagucggcaa ggacaaaaag     2520

gaugaaacau acgugcuggg gaagaucaaa gacaucuaua cgcaagaugg auacgacgcc     2580

uucaugaaga uuuacaaaaa ggauaaaagc aaguuucuca uguauagaca ugacccgcag     2640

accuucgaga aggugaucga accuauucug gagaauuacc ccaacaaaga gaugaaugaa     2700

aagggcaaag aggucccuug uaaccccuuu cuuaaguaua aagaggaaca cggguacauc     2760

cggaaguauu cuaagaaagg aaacggcccc gaaauuaagu cccugaaaua cuaugacagc     2820

aagcuccugg ggaauccuau cgauauuaca ccggagaacu cuaaaaauaa ggugguucug     2880

cagagccuua agcccuggcg caccgacgug uacuucaaca aaaauacagg caaguacgag     2940

auccucggac ugaaauaugc cgaucugcag uuugaaaaga aaaccggcac guacaagauc     3000

ucccaagaga aguauaacgg gauuaugaaa gaagagggag ucgacagcga uucugaguuc     3060

aaguuuacac ucuacaaaaa ugaccugcuu cuggugaagg auaccgaaac caaagagcag     3120

caacucuuca gauuucuguc ccggacaaug ccuaacguua aguauuacgu ggaacugaaa     3180

cccuauagca aggacaaguu cgagaaaaau gaaucccuua ucgagauucu gggcucugcu     3240

gauaagagcg ggcgcugcau caaaggacuc ggcaagucca acauuucuau cuacaaaguc     3300

agaaccgacg ugcuggggaa ucagcacauc auuaagaacg agggcgauaa gccuaaacuc     3360

gacuucuga                                                             3369


<210>  3956
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3956
augagcaacg gcaagauccu ggggcucgac auuggaaucg ccuccguggg cgucgggguu       60

auugaugcuc agaccggaga gaucauucac gccucuagca gaaucuuccc cuccgcgaau      120

gcugccaaca augccgaacg gcgcacauuu agaggcucuc ggcgccugau cagacggaaa      180

aagcaucgca uuaaaagacu ugacgaucug uucaacgacu uucacaucaa ucucgauggg      240

gagaugagca cggacaaccc uuacgugcug cgggugaagg gacuuuccca aaaacugacc      300

gucgaagaac ucuauauuuc uaucaagaau auuaugaagc gcagaggcau cagcuaccug      360

gaugacgcug aauccgauaa cgaggccggc cggagcgacu augcuaaagc cauugagcgc      420

aauagacagc ugcucacauc uaagaccccg ggggaaaucc agcuggagcg gcuugaaaaa      480

uacggacagc ugcgcggcaa cuucacaauc auugaugagg aggggcaauc ccagcagauc      540

auuaaugugu uuagcaccuc ugacuauguu aaggaagugg agaaaauccu cgauugccag      600

aagauguacc auaaguucau uuccgacgaa uuuugugaca aacugaucga gcuucugaga      660

gaaaagcgga aauauuacgu cggacccggc aacgaaaaga gcagaacaga uuacgggauc      720

uaucgcaccg acggaacgac ccucgagaau cuguucggca uucugaucgg caaaugcaca      780

uuuuacccug aucaauaucg gucuuccaga gcgagcuaca ccgcccagga guucaacuuu      840

cucaaugacc ugaacaaccu uacagugccc accgagacga agaaacuguc ccaggaacag      900

aaggaguucc ucguuaauua ugcuaaggaa acaucugugc ugggggccgg aaaaauucuu      960

caacagaucg cuaagcuggc cgauuguaaa gucgaggaca uucgcggcua ccggcucgau     1020

aacaaggaca aaccugagcu gcacaucuuu gaaaccuaua gagcgaugaa ggggcuggug     1080

ccccucgugg auaucggagu ccugagccgc gagcagcuug acauucuggc cgauauccuc     1140

acacugaaua ccgacuucga aggcauucgg gaggcucuua agaaacaacu gccuaacguu     1200

uucgacgaaa aacaggugaa ggggcucgcc uccuuuagaa agagcaaauc ucagcuguuc     1260

gcuaagggau ggcauaaucu gucccagaag aucaugcucg aggugauucc ggagcuguac     1320

gccaccagcg augaacaaau gacaauccuu acgcgccugg gcaaauuuga gaagucuagc     1380

gucgccgaau accccuccuc uauuaacgug gacgagauca ccgaugagau cuauaauccu     1440

guuguggcua aaagcauucg gcagacaauc aagauuauca acgcguccau uaaaaagugg     1500

ggcgaguucg accagaucgu caucgagaug cccagagauc gcaaugaaga cgaggaaaag     1560

aaacggauug ccgaugggca gaaggcuaac gccaaagaga aggccgacag cauccucaga     1620

gcugccgagc uguacugcgc gggaaaagug cugccugauu auguuuacaa uggccacaac     1680

caacuugcua ccaagauucg gcucugguau cagcaggggg aacgcuguau cuacacagga     1740

cagcccauuu cuauccacga ccugauccau aaucaaaacc aguaugagau ugaucacauc     1800

cugccgcucu cccugaccuu ugacgauagc cuuucuaaua aagugcuggu ccucgccacg     1860

gccaaccagg aaaaggcuca gagaacaccu uacaacuacc ugaaguccgc caccagcgcu     1920

ugguccuauc gggaguucaa agacuacgug accaagagaa aaggcauugg gaagaaaaag     1980

ugcgaauauc ugacauuuga ggaggacauc aauggcuucg aaguccgcuc uaaguuuauu     2040

caacggaacc uuguggauac cagauacgcc agcaaaguua uccucaaugc gcugcaggac     2100

uauuucaaga ucuccggcau ucagacaaaa gugucugucg ugcgcggaca auuuacgagc     2160

cagcugagac auaagugggg gaucgagaaa acccgggaaa cauaccacca ccaugccguu     2220

gaugcucuca uuaucgccgc uuccagccag cugcgccuuu ggaagaagca ggagucuccc     2280

cugguggucg acuaucaaga aggaagacag guggaucucg agaccggcga gauucuggaa     2340

cugacagacg agcaguacaa agaacuugug uaccagccuc ccuaucaagg guucguuaac     2400

accaucucca gcucugccuu ugauaaugag auucucuucu ccuaccaggu cgacagcaag     2460

gugaaccgga aaaucucuga ugcgacgauc uaugccaccc gcaaugcuca gcugggaaag     2520

gacaaaacag agggcauuua cgugcugggg aagaucaagg auauuuauac ccaggccggc     2580

uacgaggcuu uccucaaaag auauacaaag gacaaaacca gcuuucugau guaccacaag     2640

gaucuugaca cgugggagaa agucaucgaa auuauccugc gggacuaccg cgaguaugau     2700

gaaaagggaa aagagaucgg caaccccuuc gagagauacc ggcgcgaaaa uggguaugug     2760

aagaaguacu ccagaaaagg aaacggcaca gccauuaaga gccucaaaua uuacgacaau     2820

aagcugggga accauaucga uauuaccccu gaaaauucuc ggaacgccgu ugugcugcaa     2880

ucccuuaaac cguggagaac agacgucuau uuuaauaagg aaaccggaaa guacgaguuc     2940

cucggcauca aauacagcga ucuguccuuu gaaaagggga ccggcgagua uggaauuucu     3000

caggagaaau acgacagcau caagaucgcu gaaggcgugg cgaaaaaguc cauuuucaag     3060

uuuacacugu auaaacagga ucuccuguuc aucaaggaca uugagaacaa cuuugggaaa     3120

cuucugcgcu ucacgucuaa gaaugauacc agcaaacacu acguugaacu caagcccuau     3180

gacaagaaca aauuuggaac agaggaaccu cugcugcccg ugcuuggcaa ugucgccaag     3240

uccgggcaau guaucaaagg acugaacaag ucuaauauua gcaucuacaa agugcggacc     3300

gauauucucg gcuauagaca uuucaucaag caggaggggg agcacccuca gcugaaguuu     3360

aaaaaguga                                                             3369


<210>  3957
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3957
augagcaacg gcaagauccu ggggcucgac auuggaaucg ccuccguggg cgucgggguu       60

auugaugcuc agaccggaga gaucauucac gccucuagca gaaucuuccc cuccgcgaau      120

gcugccaaca augccgaacg gcgcacauuu agaggcucuc ggcgccugau cagacggaaa      180

aagcaucgca uuaaaagacu ugacgaucug uucaacgacu uucacaucaa ucucgauggg      240

gagaugagca cggacaaccc uuacgugcug cgggugaagg gacuuuccca aaaacugacc      300

gucgaagaac ucuauauuuc uaucaagaau auuaugaagc gcagaggcau cagcuaccug      360

gaugacgcug aauccgauaa cgaggccggc cggagcgacu augcuaaagc cauugagcgc      420

aauagacagc ugcucacauc uaagaccccg ggggaaaucc agcuggagcg gcuugaaaaa      480

uacggacagc ugcgcggcaa cuucacaauc auugaugagg aggggcaauc ccagcagauc      540

auuaaugugu uuagcaccuc ugacuauguu aaggaagugg agaaaauccu cgauugccag      600

aagauguacc auaaguucau uuccgacgaa uuuugugaca aacugaucga gcuucugaga      660

gaaaagcgga aauauuacgu cggacccggc aacgaaaaga gcagaacaga uuacgggauc      720

uaucgcaccg acggaacgac ccucgagaau cuguucggca uucugaucgg caaaugcaca      780

uuuuacccug aucaauaucg gucuuccaga gcgagcuaca ccgcccagga guucaacuuu      840

cucaaugacc ugaacaaccu uacagugccc accgagacga agaaacuguc ccaggaacag      900

aaggaguucc ucguuaauua ugcuaaggaa acaucugugc ugggggccgg aaaaauucuu      960

caacagaucg cuaagcuggc cgauuguaaa gucgaggaca uucgcggcua ccggcucgau     1020

aacaaggaca aaccugagcu gcacaccuuu gaaacauaua gagcgaugaa ggggcuggug     1080

ccccucgugg auaucggagu ccugagccgc gagcagcuug acauccuggc cgauauucuc     1140

acccugaaua ccgacuucga aggcauccgg gaggcucuua agaaacaacu gccuaacguu     1200

uucgacgaaa aacaggugaa ggggcucgcc uccuuuagaa agagcaaauc ucagcuguuc     1260

gcuaagggau ggcauaaucu gucccagaag auuaugcucg aggugauccc ggagcuguac     1320

gccacaagcg augaacaaau gacgauucuu acccgccugg gcaaauuuga gaagucuagc     1380

gucgccgaau accccuccuc uaucaacgug gacgagauua cagaugagau cuauaauccu     1440

guuguggcua aaagcauccg gcagaccauu aagaucauua acgcguccau caaaaagugg     1500

ggcgaguucg accagauugu caucgagaug cccagagauc gcaaugaaga cgaggaaaag     1560

aaacggaucg ccgaugggca gaaggcuaac gccaaagaga aggccgacag cauucucaga     1620

gcugccgagc uguacugcgc gggaaaagug cugccugauu auguuuacaa uggccacaac     1680

caacuugcua caaagauccg gcucugguau cagcaggggg aacgcuguau uuacaccgga     1740

cagcccaucu cuauucacga ccugauccau aaucaaaacc aguaugagau cgaucacauu     1800

cugccgcucu cccugacguu ugacgauagc cuuucuaaua aagugcuggu ccucgccaca     1860

gccaaccagg aaaaggcuca gagaaccccu uacaacuacc ugaaguccgc caccagcgcu     1920

ugguccuauc gggaguucaa agacuacgug acaaagagaa aaggcaucgg gaagaaaaag     1980

ugcgaauauc ugaccuuuga ggaggacauu aauggcuucg aaguccgcuc uaaguuuauc     2040

caacggaacc uuguggauac aagauacgcc agcaaaguua uucucaaugc gcugcaggac     2100

uauuucaaga ucuccggaau ccagacgaaa gugucugucg ugcgcggcca auuuaccagc     2160

cagcugagac auaagugggg gauugagaaa acacgggaaa ccuaccacca ccaugccguu     2220

gaugcucuca ucauugccgc uuccagccag cugcgccuuu ggaagaagca ggagucuccc     2280

cugguggucg acuaucaaga aggaagacag guggaucucg agacaggcga gauccuggaa     2340

cugaccgacg agcaguacaa agaacuugug uaccagccuc ccuaucaagg guucguuaac     2400

acgauuucca gcucugccuu ugauaaugag auccucuucu ccuaccaggu cgacagcaag     2460

gugaaccgga aaauuucuga ugcgaccauc uaugccacac gcaaugcuca gcugggaaag     2520

gacaaaaccg agggcaucua cgugcugggg aagauuaagg auaucuauac acaggccggc     2580

uacgaggcuu uccucaaaag auauaccaag gacaaaacga gcuuucugau guaccacaag     2640

gaucuugaca caugggagaa agucauugaa aucauucugc gggacuaccg cgaguaugau     2700

gaaaagggaa aagagaucgg caaccccuuc gagagauacc ggcgcgaaaa uggguaugug     2760

aagaaguacu ccagaaaagg aaacggcacc gccaucaaga gccucaaaua uuacgacaau     2820

aagcugggga accauauuga uaucacaccu gaaaauucuc ggaacgccgu ugugcugcaa     2880

ucccuuaaac cguggagaac cgacgucuau uuuaauaagg aaaccggaaa guacgaguuc     2940

cucggcauua aauacagcga ucuguccuuu gaaaagggga caggcgagua uggaaucucu     3000

caggagaaau acgacagcau uaagaucgcu gaaggcgugg cgaaaaaguc caucuucaag     3060

uuuacgcugu auaaacagga ucuccuguuc auuaaggaca ucgagaacaa cuuugggaaa     3120

cuucugcgcu ucaccucuaa gaaugauaca agcaaacacu acguugaacu caagcccuau     3180

gacaagaaca aauuuggaac cgaggaaccu cugcugcccg ugcuuggcaa ugucgccaag     3240

uccgggcaau guauuaaagg acugaacaag ucuaauauca gcauuuacaa agugcggaca     3300

gauauccucg gcuauagaca uuucauuaag caggaggggg agcacccuca gcugaaguuu     3360

aaaaaguga                                                             3369


<210>  3958
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3958
auggccaaga uccugggccu cgaccugggg accaacagca uuggaugggc ucuuaucgau       60

gacgugcaga auaaaauucu gggcgucggg uccagaaucu uccccauggg aguugagaac      120

cucggcgaug gggacggaga aauuucuaag aaugccagcc ggacaggcac gcgcggcgug      180

agacggcaau uuuuccgcag acggcugcgc aaaaaggugc uucugaaagc gcucuccgag      240

aacaauaugu gcccuaugau caccucugau uuugacaacu ggaagaagac aaaacaguuc      300

ccgagcgaaa agcuggcuuc cugguuugcc cugaaucccu acgagcucag aaacaaagcc      360

cugucugaaa aacuuacccu ggaggagauc gggcggauuu ucuaucaccu cauccagcgc      420

agaggauuuc ugagcaauuc ccggaaaggc gggacagaug acggagcuau uuucaagggc      480

aacccuaagg aagggaaaau cggaauuacc gauacacagg agagcaucca agacaagacc      540

cuuggcucuu accuguuuga aauuuauccc aaagagaauc agccuuucca gaacggccuc      600

gagcgcauca gaaaucggua cacgguccgc aagauguaug uggaugaauu ugagaugauc      660

ugggaaaaac aggcccaaua cuauuccaac cugaaugagg accugaagac ccucuucggg      720

ggaagaaaac uggauggcua caaggaagac gggauucuuu uucaucagcg gccccugaga      780

agccagaagc accucguugg aaacugugug uucgagccua acaaaacaaa gugcccgauc      840

ucugcuauuc ccuucgagca guuucgcguc uggcaauggg ugaauaccgu ugaauacaac      900

ggcaaaaaga ucucccaggu ggagaaagaa aagauugucg aguuccugua ugccaaugac      960

aagacagauu uuaaaaagau ccggaaagcg aucgggaagg aaagcaccga guucaaauuu     1020

aacuacaagg acgaugacaa gauuguggga acguauacag ugucucaucu uuccaauaaa     1080

aaguacuucg gcaaaaagug guuugaguuc agcgaaaaag agcaggaaga uaucuggcac     1140

auucuguacu uuuucgacuc caagucuaac cucaaggagu augccaucaa aaauuggaac     1200

uuuaccgagg aacaagcuga gagcauugcc aaguucaaug ucaaagaugg cuauuccagc     1260

cugucuagaa aggcuaucuc caacauccug ccuuuucuca aacugggguu cacauacgac     1320

guugccgugg ugcuuggagg cauuaagaau gucuucgggc augaauggga gaaacugagc     1380

gaugaaaagc gcaacuuucu caucgacaau guggagggaa uuguucgguc uaagaccaaa     1440

ggcggguuca ucgaugugau uaaggacauc cugagaaacg acuacaauau uagcgauaac     1500

cagcuucgca aacuguauca ccacuccgcg accaucuacg ucgccgagcu ccuggaaaag     1560

cugcccgugg gcaaagaggc ugacaaggaa auccagucua uucggaaccc uaucguuauu     1620

acagcccucu uugagcugag aaaacuugug aaugaacuga ucgaugacuu cggaagcauu     1680

gaugagauca aagucgagau ggcucgcgac cucaagaucu ccaaaagcca gcggaacaag     1740

auuagacgcg aacaaaaacg gcuggagaga gaaaaugauc gggugaaggc ccgccugcuu     1800

gaggaaggcc agagaaucac gcaugacaac auucuccugu auaagcugug ggaggagugu     1860

aaaaagaccu gccccuacac agggaauccg aucccucucu auaaacuguu uaccggagaa     1920

auucagaucg agcacaucca ucccuggucu cggucccuua acgauagcuu cgccaauaag     1980

acacuguguu acgcugacga aaacagaaag aagggcgaua agaccccuua ugaguuuuac     2040

gggaaugacg aaucuaacug guccgcgauu aaagagcgcg cccucaagcu guucagcgau     2100

acgaaagagu accccaaugc uuaucagaag uuuaaacggu ucguccaaca caaguuugac     2160

gacgauuucu ccucuagaca gcugaacgac acacgcuaca ucagcaaaga agccaagaau     2220

uaucuuucca agauuugcaa aaagguggcc guuucucccg gacaggcuac cagcaaccuc     2280

agacagaaau ggggcaugaa uaacauccug aacgaugaga augaaaagac ccgggaggac     2340

caccgccauc acgccauuga ugcgcuggug auggcuugua caaaaaucuc cuacguccaa     2400

gagcucagca aguggaacag auauaaucgg aacgccgaac ugaagaaauu uccucuuccg     2460

ugggagaccu ucuggucuga cgccaaucgc gcuguggaua agauccugau uucccauaaa     2520

aagguuagca acgacaucac agggagaacg cacauuucug aaaagaaugg caagaaguac     2580

aaaaacgugg gagucgccgc ucggggccag cuccauaagg agaccgugua ugggaaacgc     2640

acauucaaug gagaagaggc cuuucacgug agaaagacca ucgauucccu gacaaccgag     2700

aaacagcugg aaaagguugu cgacgagagc auuaaacuuc ucaucaagaa gcgggugcag     2760

gaacugggcg gguucgugaa aggagcgauu cccgccaaca cguuuuucgu cguggaugag     2820

aauggcauca agcaaccuca gaucuuucug cccaacaaaa auggggcucc uguucccgug     2880

cucaaggucc gcaugaagga aaacauuggc ggagccgaga aacugaagga gaaugugaac     2940

caguggguua acccgagaaa uaaccaccau gugcuuaucu acaaagacga aaagggcaau     3000

cugaaagagg aagucgugac cuucuggaca guuguggagc ggaagagaac cgggcaaucu     3060

gcuuaccagc ucccuuuuga uggaaaagaa auugucacaa cccugcacau caacgacaug     3120

uuccugcuug gccucaauga ggaggaaauu aacugggaga aucccgacua ugaagugcug     3180

aaggagcauc uguaccgcgu gcagaaguuu acgagcgggg auuauuacuu ccggaucgcc     3240

aaaacaucca gcauccagaa cgcgauugag aagcaacaga ucaauucuuu uggauucggc     3300

aaaaacgggu gguccaccca caauccuauu aaggucaaaa ucagcguuuc cggcaagauu     3360

ucuaaggugu ga                                                         3372


<210>  3959
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3959
augaacggcc uggugcucgg gcuggacauc ggaauuggca gcgucggggu uggaauccuu       60

aauaaggaua ccggcgagau uauccacaca aacuccagaa uuuuccccgc cgcuacggcc      120

gacaauaacg uggaacggcg caauucuaga caggcgcggc gccugaacag acggaaaaag      180

caucgcgggg cuagacucca cgaucuguuu gaggacuacg aucuucugau cgacuucagc      240

aaagugucca ucaaucucaa cccuuaucgg cugcgcgucc aaggacugga uaauccgcuc      300

accacagaag aacuguuuau ugcccuuaag aacaucguga aaagacgggg cauuucuuac      360

cuggacgaug ccagcgaaaa uggcggggcu guuuccucug acuauggaaa ggccguggag      420

gagaaccgca agcuccuggc ugaacagacc cccggccaga uccagcuuga gagauucgaa      480

aaauacgggc aaguccgggg agauuuuaca guggaggaga agggcgacaa acaucgccug      540

auuaauguuu ucagcaccuc cgccuauaga aaggaagcgg agcggauccu cagaaaacag      600

caggaauuua acaaucagau uacagauaag uucaucgagg acuaccugaa gauccugacc      660

gaaaaacgca aguauuacca cgggccugga aacgagaaaa gccggacgga cuacggcaga      720

uuucgcaccg auggcacaac ccucgacaac auuuucggga uccugauugg aaagugcaca      780

uuuuauccca augaguaccg ggccucuaaa gccuccuaua ccgcucaaga guucaaccuu      840

cugaaugauc ucaacaaucu gacggugccu acagagacca agaaacuuag cgaagaacag      900

aagaagacaa ucgucgaaua cgcuaaaucu gccaagaccc ugggcgcguc cacccuccug      960

aaauauauug ccaagaugau cgacgcuagc guggaucaga ucagagggua ccgcguggac     1020

gucaacaaua aacccgagau gcauacauuu gagguuuauc ggaagaugca gucucuggaa     1080

acgauuccug ugggagaguu cuccagagaa gugcucgaug agcuggccca cauccuuacc     1140

cugaacacag aacgcgaggg cauugaggaa gcuaucaaua ccaagcucau ggacgucuuc     1200

agccaagauc agauucugga gcuugugcag uuucggaaaa acaauuccuc ucuguucagc     1260

aagggguggc acaacuuuuc ccucaaacug augauggaac ugaucccgga gcucuacgag     1320

acaagcgagg agcaaauggc cauccugacc agacuuggaa agcagaaauu caaggaaacg     1380

ucuaagcgca caaaauacau ugacgagaag gaaguuaccg aggagaucua uaaucccgug     1440

gucgccaaau ccgugcggca ggcuauuaag aucauuaacg aagcgaccaa aaaguacggc     1500

aucuuugaua auauuguuau cgagauggcc agagaaaaca acgaggaaga cgcuaagaaa     1560

gacuauauca agcggcagaa agccaaucau gaugagaaga acgccgcuau ggagaaagcc     1620

gcguuccaau acaauggcaa gaaagaacug ccugacagca uuuuucacgg gcauaaggag     1680

cucgcuacaa agauccgccu guggcaccag cagggagaaa aaugucugua uaccggcaag     1740

aacauuccca ucucugaucu uauucacaau caguacaaau augagaucga ccauauccuc     1800

ccucugagcc uguccuucga ugacucucuc agcaacaagg ugcugguccu ugccacagcc     1860

aaucaagaaa aagggcagag aacgcccuuu caggcucugg auuccaugga cgaugccugg     1920

agcuaccggg aguucaaguc uuacgugaag gacuccaaac uccugggaaa caagaaaaag     1980

gauuaucugc uuaccgagga agacauuagc aaaaucgagg ucaagcagaa guuuauugaa     2040

agaaaucucg uggauacacg cuacucuucc cggguugugc ugaacacccu gcaagacuuc     2100

uauaaagcuc acaaguuuga cacaaccauc agcgucguga gaggccaguu cacgucccag     2160

cuccgcagaa aaugggggcu ggagaagucu cgggagaccu accaucacca ugccguugau     2220

gcgcuuauua ucgccgcuag cucccagcug cgccucugga aaaagcaagc caauccgcug     2280

aucucuuaua aggaagacca guucguggau agcgagacag gcgaaauuau cucccugacc     2340

gacgauaaau acaaggagcu ugucuuuaaa gcuccuuaug cccacuucgu ggacacacuc     2400

agcucuaaga aauuugaaga uuccauucug uucagcuacc agguggacuc uaaguacaac     2460

agaaaaaucu ccgaugcgac cauuuaugcc acgcggaagg uuaagcugga caaagauaag     2520

agcgaggaga cauacguccu cggaaaaauc aaggacaucu auucucaagc uggcuacgau     2580

gccuuuauua aaaucuauaa uaaggacaag agcaaauucc ugauguacca caaggacccc     2640

cagaccuuug aaaaagugau ugaggaaauc cuucgcacau auccuuccaa ggaguucaac     2700

gagaaaaaua aggaagugcc cugcaacccc uuugagaagu acagacagga aaacgggccu     2760

auucggaaau acagcaagaa aggaaauggc ccggagauca agucucugaa auauuacgau     2820

aagaacauuc ccgaauccuu caucaauauc accccuagca acuccaagaa uaaagucauu     2880

cuccgccagc ugaaccccug gagaaccgac guguauuuua aucaucaaac agagaaguac     2940

gaacugcuug ggcucaaaua ugcugaucug caguucgaga agaaaacggg aaccuacucu     3000

aucacaauug acaaguauaa cgagaucaag gaaaaagagg gcguugggga acagagcgag     3060

uuuaaguuca cccuguacaa aaaugaucuc cugcuuauua aggacaccaa aaacaaugag     3120

aagcagaucu uccgguuucu gucccgcaca augccuaacg ugaaacacua cgucgaacuc     3180

aagcccuaug auaaggccaa auucgacggc aaucaaccgc ugaucacgau uuuuggaaac     3240

guggccaagg gcgggcagug ucugaaagga cuuaauaagu cuaacaucag cauuuucaaa     3300

guuaagacag augugcuggg caacaagcau uuuaucacca aagaggggga aaagccuaaa     3360

cucaaguucu ga                                                         3372


<210>  3960
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3960
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

aauaaggaga ccggagaaau uauccacgug aacuccagaa uuuuccccgc ugccacagcg      120

gauucuaaug uggagcggcg cggcuuuaga caggggcggc gccugggaag acggaaaaag      180

caucgcagcg cuagacucaa cgaccuguuc gaagaguuug gcuucaucac ggauuuuucc      240

gccgucccuc uuaaucugaa cccguacgcc cuccggguga aaggccuguc ugaagaacug      300

accaaugagg aacucuucau cgcucugaag aacauuauca aacgcagagg gauuagcuau      360

cuugacgaug ccuccgagga cggagaaaca gcuucuaaug aguacggcaa ggccguugag      420

gaaaaccgga agcugcucgc ggauaaaacc cccgggcaaa uccagcugga gcgcuuugaa      480

aaguauggac aggugagagg cgacuucaca gucguggaga auggggaaaa ccaccggcuu      540

auuaauguuu uuagcaccuc cgccuacaaa aaggaggcug agcgcauccu gagacggcag      600

caagaguuca acgugagaau uagcgaugag uuuaucgaag ccuaucucac aauccugacc      660

ggaaaacgca aguacuauca uggcccuggc aaugagaagu cucggacgga cuacgggaga      720

uuccgcaccg auggaacaac ccuggacaac auuuuuggca uccucauugg gaaaugcaca      780

uucuaccccg augaauaucg ggcugccaag gcguccuaca ccgcccagga guucaaccug      840

cuuaaugacc ugaacaaucu cacggucccu acagagacca aaaagcugag acccgaacag      900

aaacgccaga ucguggagua ugcucggaca gccaagaccc uuggaacccc uacacugcuc      960

aaauacauug cuaagcuggu ggacggcagc aucgaugaca ucaaggggua uagaauugau     1020

aaaucugaca agccggaaau gcacacguuu gaugccuacc gcaaaaugcg gacccuggag     1080

cucgucgacg uugauauccu guccagagaa acacuugacg aucuggccua uauucucacc     1140

cugaacacag agagcgaggg aauccuugaa gcucucaauu cuaagaugcc cggcaccuuc     1200

acgaaagagc aaauugacga acugauccag uuucgcaaga agaacuccgc gguguucggc     1260

aaaggguggc auaauuuuag ccugaagcug augaacgagc ucaucccuga gcuguacgaa     1320

acauccgagg aacagaugac cauucuuacc cggcugggaa aacagagauc ucgggagauc     1380

agcaagcgca caaaauacau ugaugaaaag gagcucaccg acgagaucua uaaucccgug     1440

gucgccaagu ccgugagaca agcuauuaaa aucauuaacc uggccacaaa gaaauacggc     1500

aucuucgaca auaucguuau ugaaauggcc cgggagagca acgaagauga cgagaagaaa     1560

gcuauccaga augugcagaa ggccaacgaa gaugagaaga aagcggcuau ggagaaggcc     1620

gccgaccugu auaaugggaa aaaggaacuu ccugauucua uuuuucacgg acacaaagag     1680

cucgcuacga agaucagacu guggcaucaa cagggcgaac gcugucugua caccgggaaa     1740

aacauuucca uccacgaccu cauccauaac ccccaccagu augagauuga ucacauccug     1800

ccgcuuagcc ugucuuucga cgauggccuc gccaauaagg uccuggugcu ggcuacagcc     1860

aaccaggaaa agggacaacg gaccccuuuu caggcgcuug acagcaugga ugacgccugg     1920

uccuacauug aguucaaaca guauguuaga aauucuaaga gccucuccaa caaaaagaaa     1980

gauuaccugc ugacagagga agacaucagc aagauugagg ugaagcagaa auuuaucgaa     2040

cgcaaucucg ucgacaccag auacucuucc cggguggucc ugaacacgcu ucaagaguuc     2100

uauaagacca augauuuuga cacaaaaauc agcgugguuc gcggccaguu caccucucag     2160

cugagacgga aguggaaaau ugagaagucc cgcgauacau accaucacca ugcuguggac     2220

gcccucauca uugcugccag cucccagcug agacugugga agaaacaaaa caauccccuu     2280

aucucuuauc gggaagggca gcucgucgau ccugagaccg gagaaauucu gagccugacg     2340

gacgaugagu acaaggaacu cgugcugcgc ccucccuaug acuacuucgu ugauacacuu     2400

aaauccaagu cuuuugagga cagcauccug uucuccuauc agguggauag caaauacaac     2460

agaaagaucu cugacgcgac cauuuacggc acacggaagg cccagcucgg gaaagauaag     2520

caagaggaaa ccuauguccu gggaaaaauc aaggacauuu acucccagaa aggcuaugag     2580

gacuuuauca agcgcuacaa aaaggauacc acacaguucc ugauguauca caaggacccc     2640

caaacguuug cuaaagugau ugaagagauc cuuaagaccu acccggauaa agaacucaau     2700

gagaagggga aagagauucc cugcaacccu uucgaaaagu auagacagga gaauggcccc     2760

auccggaagu acagcaaaaa gggaaaaggc ccugaaauca agucucugaa auacuaugac     2820

aacaagcugg ggaaucauau ugauaucaca cccgugaacu cccagaacca gguuguccuc     2880

caaagccuga agccguggag aaccgacgug uacuuuaauc cucagacauc uaaauaugag     2940

cuuaugggac ugaaguacag cgaucuccgc uucgaaaaag gcuccgggag cuauggaauu     3000

ucucccgaga aguacaacaa agugaaggcc aaggagggcg ucgacgaaga uuccgaguuu     3060

aaauucaccc uguauaagaa ugaccugauc cuuauuaaag auacggaaac aggggagcag     3120

cagcuguuuc gguacggcag cagaaacgac accuccaagc acuacgugga gcucaaaccu     3180

uaugaaaagg cuaaguucga gggaaaucaa cagcugauga accuccuggg caccguugcc     3240

aaagggggac agugucuuaa gggcaucaau aaacccaacc ugucuaucua caaggugaaa     3300

acagacgucc ucgggaauaa guauuuuauu aaaaaggaag gagaucagcc ucaacugaac     3360

uucaagaaaa agaucuga                                                   3378


<210>  3961
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3961
augaccagca agcaggagaa ccugggcgcc cucacauucg ggcuggacau cggaauugcu       60

uccgugggcu gggccguccu uggggaaaau agaaucauug aucugggagu ucggugcuuu      120

gacaaagcgg agacggcuaa ggaaggcgag ucucucaacc uggcccgcag aauggcccgg      180

cuucugcgca gacggcuccg cagacgggcu uggcgccuga ccaaacuggc cagacuccug      240

aagcgggaag ggcuuaucgc ugaugccaau cuguucaaaa acaagauuga gaagaauaca      300

cccgugccua aaagcuccug gcaacuccgc guggacgcgc uggauagaaa gcugaacgcc      360

gaggaauggg cucgggucau cuaccaccug uguaaacauc gcggauuuca cuggaucucu      420

agagccgagg aaaaggcugc cgagagcgac uccaaaggcg agucuggcaa ggugaagcag      480

gggcucgcgg gaaccaaaaa gcugaugcag gaaaagaauu aucggacagc cgcugagaug      540

guucuggccg aguucccgga agcucagaga aacaagcaag gcgauuacag caaagcccuc      600

ucccgcauuc ugcuugggga cgaacugaag acccucuuug agcaucagcg gaaacuggau      660

agccccuucg ccacagagaa acuugaagcu gcgauccugg gaaauggcga cagaaagucu      720

gggcucuuuu gggcccagaa cccugcucug gccggauccg aucugcucaa aaugcugggc      780

uauugcaccu ucgagaagac ggaauaccgc gcccccaaag cuagcuuuac cgccgagcgg      840

cacguguggc uuacaagacu gaauaaccuc cgcauugucg uggacggcac cacacggccu      900

cugaaugaag cggagagacg caucgcccuu cugaugcccu aucagcaagc cggggauuuc      960

accuacaagc agcuccggac ggcccugaca aaagcuggau uucugccuga gucuuucaga     1020

uauaccggcc ucuccuacgc cagcgggcag aaggcuggag aaaagacaaa agacccggag     1080

ucugccgcgc ugguuaaguu ucccgccugg caggaacuuc gcaaaacccu ggagaagaaa     1140

gaacucgcuu ccgaguggca aaagauuagc accgacgccc uggauggcaa gccucagcug     1200

cuugaccaga ucgcuugggu gcucuccguc uacaaagaug acgaugaggu gaaggccgaa     1260

cugucuaaac ugaaccuccc caaugcggag aagaugauug acgcccugcu ugauauccgg     1320

uucgacaaau ucagcaaccu gucccucaag gcucugugua agaucgugcc ucugauggaa     1380

caagggcaga gauaugauga ggccauugcu aacauccccg aguacggaca ucacagccag     1440

cuucacaaag ucggcgccgg cgaacauaag uaucucccgc cuuuuuacaa agagcgggac     1500

aagacagggc gcaugguuuu caaugaugac auggacauuc ccagaaaccc uguggugcug     1560

cgggcccuga aucaggcuag aaaagucgug aacgcgcuca ucaagaaaua ugauucuccc     1620

cacgaaguuc auauugagau ggcccgcgac cugucccggc cccuugauga aagacgcgag     1680

guggagaagc ugcaaaagga auuuagagac aauaacgaga aaggaaaggc ucaguucgcc     1740

caggauuuua auaucgucgg cgccgugaaa gggaaggagu ucgaaaaaua ccagcucuau     1800

cgggaacaac agggaaagug cgcuuacagc aucgagccua uggacauuca ccgccuguuu     1860

gagcagggcu acguugaaau cgaucacgcc cugccguauu cucggagcuu cgacgauucc     1920

aagaacaaua gagugcuugu ccucgcgaaa gagaaccgcg acaaggggaa uagaacgccc     1980

uuugaauacc ugggcggaaa agauaacucu gagccuuggc ggcaguucgu ggcuuuuguc     2040

gaaggcaaua aggccuaucg ccuggccaaa agaagccggc uccugcgcaa ggacuucggg     2100

gauaaggagg cuaaagaguu uagagaacgg aaccuuaaug acaccagaua cauuuguaag     2160

uucuucaaaa acuaugugga gcaauaccug cagcuccaug aagacuccga ggccaagcgc     2220

ugcguugugc ugagcggaca gaugacaucu uuucugcggg cgagaugggg ccuugucaaa     2280

gugcgcgggg auuccgaccg gcaccaugcu cucgaugccg ccguuguggc ugccuguagc     2340

cacggaaugg ucaagagacu gucugacuau ucccgccgga aggagcugga acaggugaga     2400

gagggcuucg uggauguuga caccggggaa aucgucaacc ccgcuauguu ucaacaggug     2460

caggagcauu ucccuaaucc cuggccgcac uuucgcgaug aagugaaacu ccggcugagc     2520

auggacgauc cugcccuucu gagauccgag auggagaagu ucggcacaua cacccccgaa     2580

gcgcuccaag cccugcaccc ucuguuuguc ucucgggcuc cccagcgcag aaacagcgga     2640

gccgcucaua aagacacgau uuacgcccag ccuaagcggc uuguggccca gggcgggguu     2700

acacaaaaag uggcucugac cucccucacc cugaaggauc ucgacaaacu gaucgauccc     2760

caccgcaaug acaaacuuua uacagcgauc agagcccggc uggagcagca uggaggcaag     2820

ggggacaagg cuuucccguc uagcaacccu cucagaaagc ccgauuccga aggaaauccc     2880

accggcccua uuguccgcgg ggugacaaug guuaucgaca aacugagcgg cauuccgauc     2940

cggggaggca uugccaagaa cgauucuaug gugagagucg acguguuuac gaaagccaau     3000

aaguuccacc ugguucccgu guacguccac cauaccgugg cuaaggcccu uccuaaccgc     3060

gcgguggucg cuuuuaaaga ugaggacgaa uggacacuga ucgaugacuc cuucgauuuu     3120

ugcuucagcc uccaccccaa ugaccugguu caggugcagc aaaaggggaa agccggaaug     3180

auuggcuauu acucuuccug ucaucggggg accggaaaca ucaaucucug ggcccacgau     3240

agaaaccuuc aggugggcaa ggacgggaug aucgagggca uuggagucaa aacagcucuu     3300

agccuggaga aguucaaugu ggacguucuc ggcaaccgcu auccugcucc cccugaaaag     3360

cggagagauc uggcguga                                                   3378


<210>  3962
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3962
augaccgaca gaaucagccu gggccucgau auuggggugg ccuccgucgg auucucuguu       60

cuggacaucg auaagggcaa agugauugag cuuggggcuc ggcuguuuaa cgccacagug      120

gcggcuggaa aucaggaccg cagagauaug cggggcagcc gcagacuccu gaaccggaau      180

aagcaacgca gaaaagacgu cuccaagcug uuccaggaau acgggcugcu cgauaacuuu      240

gaccgggaua auuuccacac ggccuuuaac aauaacugga aucccuauga gcugcgcgug      300

aaaggacuga ccaagcagcu caacaaggaa gaacuggccg acucucuuua ccagaucauu      360

aaaagacggg gcaucagcua ugcucugaag gaugccgacg cuaaugaaga uggcacagac      420

uacuccucua gccucaaaau caacucccaa gagcugauug agaagacccc ugcccagauc      480

cagcuucagc gccugaauga cuaugggaaa guuagaggaa aggugauugu cggcgaugac      540

aucgaugacc aaaaggugcu ccugaacguu uucccgacaa augcguacga aaaagaggcc      600

cggcagauua ucgcuaccca gcagcaauuu uaucccgaaa uucugacagg ggaguucgug      660

aaggaguacu gccagauccu cacccgcaaa agagauuacu uugucggacc uggcaacgaa      720

aagagccgga cggacuaugg gaucuacaaa accgauggaa gaacacugga caaccuuuuc      780

gaggaacuga uuggccauga uaagaucuau cccgacgagc uccgcgccuc ugcugccucc      840

uacaccgcgc agcuguuuaa ugugcuuaac gaucugaaua accuccggau ucugagcuau      900

gaagacggca aacugacaca aaucgacaag gagaagauua ucgccgagau caaaaauaac      960

accacggugg ucaauaugcu caacguuauu aagaaaguga caggguguca gaaggaugac     1020

aucaaaggau ucagacugaa ugaaaaggau aagccugaga uuucuuccau gcccaucuac     1080

cgcaaagcuc acaaggacuu ucuuaaagug ggcauugaua ucaccgacug gccuaucgau     1140

uucauugacc ggcugagcuu cauccucaca cugaacaccg aaaaugggga gauuagaaag     1200

cagaucaaca aacgccuugu cccggauuuu gacuuccuga augaugaccu cgugcagcug     1260

auuaucgaua acaaggacuc uuuugacauu aagaccaaua acaaauggca ucgguucucc     1320

cugaagacaa ugaacaaacu cauccccacg augaucgaaa gaccuguuga gcaaaugacc     1380

cugcuuacag agaugggacu gauuaagaaa gauaccaagc gcuuugaagg caauaaguau     1440

cuccccuaca aagagaucgc caacgacauu uucaauccug uggcuagcaa guccguccgg     1500

gaagcccuga aaaucgugaa cgccguucug aagaaauacg ggcacauuga guauaucgug     1560

aucgaaaugc ccagagauaa gaaucuugac gaggagcgca aacagauugc ugauuuucag     1620

aagaagaaca aaaagaucaa agacgcggcc uucaaugcuu uugucaaggc cgugggaucu     1680

aaaaagaacg ucaagauugc ccucagcaaa aaucggaagc ugcagauggg caucagacug     1740

uggcaucaac agcagggcau ugauccguac aacggggaag agaucaaugc uaacgaccuc     1800

aucuccaauc cugauaaauu cgaaauugac cacaucauuc cccagagcau cucuuuugau     1860

gacggaauua acaauaagac acugugcuuc gccuccauga accaagugaa aggccagaag     1920

accccuuaug aguuucuuaa cgaggggcac ggacagggcu acgcgaaguu caaagcuauc     1980

guuagcaaga auaaaaacuu cucuaaggcc aaacgggaua auuaucuguu ugaagagaac     2040

gugagcaaua ucgaaacgcg caagagauuc cucucccgga accuggucga cacaagauac     2100

ucuagccgcg ugguucuuaa uucccuucag gaguuuuucc augaaaagag cgacgauacc     2160

aaagugaccg ucauucgggg gaaguuuaca ucuaacauga gaaaacacug gcauaucgac     2220

aagacccgcg auacauucca ccaccaugcc auugacgcuu ccaucauugc cgcuacgccc     2280

uuucucagaa uguggaaaaa gggcagcacc aucuucccca caaaaauuaa ugagaccucu     2340

aucgauaucg agacaggaga aauucuggac gauaagaacu uugacaaggu gauguaugag     2400

gaaccuuacu ccggcuucau cagcgagauu augaaugccg augaccggau caaauuuucc     2460

caccaagugg auaagaaaau gaaccgcaag guuucugacg cgaccauuua cagcacgaga     2520

aucgggaaac uguccaagga caagaaagau gccgaauaug ucguggcuaa ggugaaagac     2580

aucuacucug ucgauggaua ugagaauuuc aagaaagugu acaagaagga caaaaccaag     2640

uuucuccugu auaaauacga uccgcggaca uucagcgagc uugaaaagau uguuaucgac     2700

uaucccgaua aaauugagaa ggugcagacc aacggcaaga ucaaagccgu cgacauuucc     2760

ccuuucgaac uguaccgcag agauaauggg auggugcgga aguacagcaa aaagacagga     2820

cccgcuauca aacagcucaa guaucuggac aagaaacugg gcucucauau cgauauuacc     2880

ccuaagaacg ccaacaauaa acacguugug cuucaauccc ucaagcccug gcgcacggac     2940

gucuaccuga accaugagac aggggaauau gagaucaugg gcauuaaaua cagcgaucug     3000

aaguuuaaua aaaacgaggg auauggcauc aagaaggaca aauaccucaa gauuaaaaau     3060

aucgaagggg ugucugagaa cuccgaguuc auguuuagcc uguauagaaa ggaccggauu     3120

aaaguucagg auauggagac caaugagucu guggaacuuc uguucuggag ccgcaacuuu     3180

uccaauaaga aguacgcgga gcucaaaccg aucagccaga ucgacaacga uaagguccug     3240

ccuauuuacg gaagaggccg gcugaucaaa agacuuauuc ccaagaauug uaaaaucugg     3300

aaggugaaca caucuauucu cggggacacc uauuaccugg aaaaggaguc caauuauccu     3360

cagaaaaucc uggauuga                                                   3378


<210>  3963
<211>  3381
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3963
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

aauaaggaga ccggagaaau uauccacgcu aacuccagaa uuuuccccgc cgcgacagcu      120

gauucuaaug uggagcggcg cggcuuuaga caggggcggc gccugggaag acggaaaaag      180

caucgcagcg ccagacucaa caaucuguuc gaagaguuug gcuucaucac ggacuuuucc      240

gccaucccuc uuaaccugaa uccguacgcu cuccggguga aaggccuguc ugaagaacug      300

accaacgagg aacucuucau ugcccugaag aauaucauua aacgcagagg gaucagcuau      360

cuugaugacg cuuccgagga uggagaaaca gccucuaacg aguacggcaa ggcggucgag      420

gaaaaucgga agcugcucgc cgacaaaacc cccgggcaaa uucagcugga gcgcuuugaa      480

aaguauggac aggugagagg cgauuucaca guuguggaga acggggaaaa ucaccggcuu      540

aucaacgucu uuagcaccuc cgcuuacaaa aaggaggccg agcgcauucu gagacggcag      600

caagaguuca augugagaau cagcgacgag uuuaucgagg cuuaucucac aauucugacc      660

ggaaaacgca aguacuauca uggcccuggc aacgagaagu cucggacgga uuacgggaga      720

uuccgcaccg acggaacaac ccuggauaac aucuuuggca uucucaucgg gaaaugcaca      780

uucuaccccg acgaauaucg ggccgcgaag gccuccuaca ccgcucagga guucaaucug      840

cuuaacgauc ugaauaaccu cacgguuccu acagagacca aaaagcugag ccccgaacag      900

aaaagacaga uuguggagua ugcccgcaca gcuaagaccc uuggaacccc uacacugcuc      960

aaauacaucg ccaagcuggu cgacggcucu aucgacgaua uuaaggggua ucggaucgac     1020

aaauccgaua agccggaaau gaugcacacg uuugacgccu acagaaaaau gcgcacccug     1080

gaucucguga auauugacgc ucugagccgg gagacacuug augaccuggc gcauauccuc     1140

acccugaaca cagaaucuga gggcauucuu gaggcccuca auuccaagau gcccagcacc     1200

uucacgaaag aacaaaucga ugagcugauc caguuuagaa agaagaacuc cgcuguguuc     1260

ggaaaaggcu ggcacaauuu uucucugaag cugaugaacg aacucauuag cgagcuguau     1320

gagacauccg aagaacagau gaccauccuu acccgccugg ggaaacagcg gagcagagaa     1380

auuucuaagc ggacaaaaua caucgacgag aaggaacuca ccgaggagau uuacaauccu     1440

gucguugcca aguccgugcg ccaagccauc aaaauuauca acgaagcuac aaagagauau     1500

ggaaucuucg auaauauugu gaucgagaug gcccgggaaa acaaugagga agacgcgaaa     1560

aaggacuaca uuaaaagaca gaaggcuaac caggaugaga agaacgccag cauggagaaa     1620

gccgcuuuuc aauauaaugg caagaaagaa cugcccgacu cuaucuucca cgggcauaag     1680

gagcuggcca cgaaaauucg ccuuuggcac cagcagggag aacggugucu cuacaccggc     1740

aagaacauca gcaucagaga ucugauucau aauccucacc aguaugagau cgaccacauu     1800

cugccccucu cccugucuuu ugaugacggg cuugcuaaca aaguccuggu gcucgccaca     1860

gcgaaucaag aaaagggcca gcgcaccccg uuccaggcca ucgauagcau ggacgaugcu     1920

ugguccuaca gagaguuuaa gcaguauguu cggaacagca aaucucuguc caauaagaaa     1980

aaggacuacc ugcuuacaga ggaagauauu agcaaaaucg aggugaagca aaaguucauc     2040

gaacgcaacc ucgucgacac cagauacucu ucccgggugg uucugaauac gcugcaggag     2100

uuuuauaaaa ccaacgauuu cgacacaaag auuagcgugg uccgcggaca guucaccucc     2160

cagcucagac ggaaauggaa gaucgagaaa ucucgcgaca cauaccauca ccaugccgug     2220

gaugcucuga uuaucgccgc gagcucccaa cuuagacugu ggaagaagca gaauaacccu     2280

cucauuucuu auaaagaagg ccaguuuguc gaccccgaga ccggggaaau ccugagccug     2340

acggaugacg aguacaagga acuuguguuc cggccucccu augauuacuu uguugacaca     2400

cucaaaucca agagcuucga ggauucuauc cuguuuuccu aucaggugga cagcaaauac     2460

aaucgcaaga uuucugaugc caccaucuac ggaacaagaa aggcucaacu gggcaaagac     2520

aagcaggagg aaaccuaugu ccucgggaaa auuaaggaua ucuacuccca gaaaggauau     2580

gaggacuuca uuaagcggua caacaaagau gaaacccaau uucugaucua ucacaaggac     2640

ccccagacau ucgagaaggu gauugaagag auccuuaaaa cguacccuga caaggagcug     2700

aaugaaaaag gcaaggagau cccgugcaac cccuuugaaa aauauagaca ggagaacggg     2760

ccuauucgca aguacagcaa gaaaggcaag ggacccgaaa ucaaaauucu caaguacuau     2820

gauaauaaac ugggcaacca uaucgacauu accccuguua auucucagaa ccaagugguc     2880

cugcagagcc uuaagcccug gcggacagau guguacuuca auccgagaac cuccaaguau     2940

gagcucaugg ggcugaaaua cagcgaccug cgcuuugaga agggaucugg cuccuauggg     3000

aucagcccug aaaaauacaa caaggugaaa gccaaggagg gaguuaauga agauuccgag     3060

uucaaguuua cacucuauaa aaacgaccug auccuuauua aggauaccga gacgggcgaa     3120

cagcagcugu uccgguacgg gucuagaaau gacacaagca aacacuacgu cgagcucaag     3180

cccuaugaaa aagcuaaguu cgagggcaac caacagcuga ugaaucugcu uggaaccgug     3240

gccaagggcg ggcagugucu gaaaggaauc aacaagccua aucucuccau uuacaaagug     3300

aagaccgaug uccugggcaa caaauauuuu aucaagaaag aaggggacca gccccaacuc     3360

aauuucaaga agaaauuuug a                                               3381


<210>  3964
<211>  3381
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3964
auggccagca agcacgagaa ccugggcgcu cucaccuucg ggcuggacau cggaauugcc       60

uccgugggcu gggcgguccu uggggaaaau agaaucauug aucugggagu ucggugcuuu      120

gacaaagcug agacagccaa ggaaggcgag ucucucaacc uggcccgcag aacggcucgg      180

cuucugcgca gacggcuccg cagacgggcc uggcgccuga ccaaacuggc uagacuccug      240

aagcgggaag ggcuuaucgc cgauacaaau guguuccaga aacccuuuaa ccugaaggac      300

gcgaaugaga agggacucag cuucuggcag cugcgcgugg augcccuuga cagaaaacug      360

uccgcugagg aaugggcccg ggucauuuac caucucugua agcaccgcgg cuuucauugg      420

aucucuagag cugaggaaaa agccgcggag ggcgauagca aguccgaagg gggaaaagug      480

aagcagggcc uggccgggac caagaaacug augcaggaaa agaacuaucg gacagcugcc      540

gagaugguuc ucaccgaguu cccugaggcu cagagaaaua aacaaggaga cuacucuaag      600

gcccugagcc gcauccuucu gggcgaugaa augacacagc ucuuuaaaca ccagcgggag      660

cuggggaacc cgcaugccac cgagaaacuu gaagcugcga uucugggaaa uggcgaccac      720

aaauccggcc ucuucugggc ccagaacccc gcucuggccg ggaaugaucu gcucaagaug      780

cugggacacu gcacguuuga gaaggccgaa uauagagugc cuaaagcuag cuucaccgcc      840

gagcgccaug ucuggcuuac acggcugaac aaucucagaa ucgugguuga cggcaccaca      900

cgcccccuga acgaagcgga gcggcaccuu gcucugcuca ugccuuacca acaggccggg      960

gaucugaccu auaagcagcu gagaacggcc cucacaaaag cuggauaccu gcccgagucu     1020

uuucgcuaua ccggccuugc cuacgcuucc gggcagaagg ccgacgaaaa agcgaaggac     1080

ccugagagcg ccccgcuggu gaagcucccc gcuuggcaug aacugcggaa aacacuggag     1140

aagaaagaac uugccucuga guggcaaaag auuuccagcg augcucucga cggaaaaccu     1200

cagcugcugg aucagaucgc cuggguccuc ucuguguaca aggacgauac cgagguggaa     1260

gcggagcugu guaaacuuaa ucugcccaac gccgaaaaga ugauugacgc ucuccuggau     1320

aucagauucg acaaguuuuc caaccugagc cuuaaggccc uccgccaaau ugucccucug     1380

auggagcagg gccagcggua ugaugaggcu guugccaaua uccccgaaua cggccaccac     1440

ucccagcugc augagguggg ggccggagaa cacaaguauc ucccgccuuu cuacaaagag     1500

agagacaaga ccggccggau gguguucaac gaugacaugg acaucccccg caauccuguc     1560

gugcugagag cucuuaacca agcgcggaag guugugaaug cccugauuaa agaauauggg     1620

ucuccccaug agguccacau cgagauggcu agagaucuca gccgcccccu ggacgaacgg     1680

agagaugugg agaagcugca gaaagaauuu cgcgacagaa acgagaagga uaaagcccuu     1740

uucgcccagg acuuuaauau uguuggagcu gugaagggca aagaguucga aaaguaccag     1800

cucuaucggg agcaacaggg gaagugcgcc uacuccaucg aaccuaugga uauucaucgc     1860

cuguuugagc agggcuacgu cgaaaucgac cacgcgcugc cguauagcag aucuuucgau     1920

gacuccaaaa acaaucgggu gcucguccug gcuaaggaga accgcgauaa aggaaauaga     1980

acacccuuug aauaccuugg cgggaaggac aacagcgagc gguggcgcca guucguggcc     2040

uuuguugagg gaaauaaauc uuauagacug gccaagcgga gccgccuccu gagaaaggau     2100

uucggcaaag acgaagcuaa gggguuucgg gagagaaacc ugaaugacac gcgcuacauc     2160

ugucgguucu uuaaaaacua uguggaacaa uaccuucagc uccacgagga uuccgacgcc     2220

aagagaugcg ucgugcuguc uggacagaug accagcuucc ugcgcgcgcg guggggccuc     2280

guuaaaguga gaggggauuc cgaccgccau cacgcucugg auggcgccau ugucgccgcu     2340

uguagccauu cuauggugaa gcggcuuucc gacuauagca gacgcaagga gcuggaacag     2400

gugcgggccg gauucguuga ugucgacaca ggcgagaucg ugaacccugc uauguuucaa     2460

caggugcagg aacacuuccc caauccuugg ccccacuuua gagaugaggu caaacuccgg     2520

cugaacgugg acgauccggc ccugcuucau ucugaaauga aucgcuucgg gaccuacucc     2580

ccugaggcgc ugaaggcccu cagaccccug uuuauuagcc gggcuccuca acgcagaaac     2640

uccggagccg cucacaaaga cacaaucuac gcccagcccg agcggcucgu ugcccagggc     2700

ggggugaccc agaagguccc ucugacgucu cuuacacuga aagaucucga caagcugauu     2760

gacccccaua gaaaugaaaa gcuguauagc gcuauccgca uccggcuuga gcaacacgga     2820

ggcaaagggg auaaggcguu cccggccucc aacccucuga gaaaacccga cucugaaggc     2880

aaucccaccg gaccuauugu gcgcggcguu accaugguga ucgauaagcu cagcgggauu     2940

ccgauccggg gaggcauugc uaaaaacgac uccaugguca gaguggaugu uuuuacaaag     3000

gccgggaaau uccaucuggu gcccgucuac gugcaccacg ccguggcuaa gguccucccu     3060

aaucgcgccg uuguggcgua uaaggacgag accgaaugga cacugaucga ugacggauuu     3120

gauuucugcu uuagcaugua ccccaacgac cuucugcgga uuaaacucag aaaugcuucu     3180

caucugggcu auuacgccuc cuguaaccgc agcacggggg ccaucaaucu guggucucac     3240

gauagaaacc aacagguggg caaggacgga augaucgagu ccauuggcgu caaaaccgcu     3300

cuuaaugugg agaagcugaa caucgacguu cucgggaacc gguauccugc gcccccugaa     3360

aaacgcagag aucuggccug a                                               3381


<210>  3965
<211>  3384
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3965
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaaug uggagcggcg caucaacaga caaggccggc gccugacaag acggaagaaa      180

caucgcagag uccggcucaa ucaccuguuu gaagagagcg ggcuuaucac ggacuucacc      240

aacgugucca uuaaucugaa cccuuaccag cuccgcguua agggacugac agaugaacug      300

ucuaaugagg agcucuuuau cgcgcugaaa aacaugguga agcauagagg cauuagcuau      360

cuugacgaug cuuccgacga uggcaauucu agcgucgggg acuacgccca gaucgugaag      420

gaaaacucca aacagcugga gaccaagaca ccgggacaaa uucagcucga acgguaucag      480

aaauacggcc agcugcgcgg ggauuucacc guugaggagg acggaaagaa acacagacuu      540

aucaaugugu uucccacaag cgccuaucgg gcugaagccc ugcgcauucu ccaaacccag      600

caggaguuca acccucagau cacggaugaa uuuaucaauu cuuaccugca aauucugacc      660

ggcaagagaa aguauuacca ugggcccgga aacgagaaau cccggacaga cuacggcaga      720

uauaccacaa agaaagauag cgaagacgag uacaucaccc ucgacaacau uuucggcauc      780

cugauuggga agugcacguu uuauccugag gaauaccgcg cugccaaagc gucuuauaca      840

gcccaggagu ucaaucuucu gaacgaucuc aauaaccuga ccguccccac agaaaccaag      900

aaacuuuccg aggaacagaa gaaucaaauc aucaccaacg ugaagaauga gaaagcuaug      960

ggaccugcca agcuguuuaa auacauugcu aagcuccuga gcugugacgu ggccgauauc     1020

aaaggcuauc ggauugacaa gucugauaag gcggagaucc acacauucga agccuacaga     1080

aaaaugaaga cgcuggcuac cauugacauc gagcagaugg aacgcgagaa ccucgauaaa     1140

cuggccuacg uccuuacacu gaauaccgaa aaggagggga uccaggaggc ucucgaacac     1200

gaguuugccg acggaacauu cucccaggaa caaauugaug agcugguuca guuccggaaa     1260

gccaacagcu ccaucuuugg caaggggugg cauucuuuca gcgugaaacu uaugauggag     1320

cugauuccgg aacucuaugc uaccuccgag gaacagauga cgauccugac aagacuggga     1380

aagcagaaga ccaccucuag cuccaauaaa acaaaguaca uugacgagaa acaacucacc     1440

gaagagaucu auaaccccgu ggcggccaag agcguccgcc aggcuauuaa gaucgugaau     1500

gccgccauca aagaguacgg cgauuuugac aacauuguua ucgaaauggc ucgggagaca     1560

aaugaagacg augagaagaa agccauucag aagauccaga aagcgaacaa ggacgaaaaa     1620

gaugcugcca ugcugaaggc cgcuaaccaa uauaauggca aggccgagcu uccucacucu     1680

guguuccaug ggcacaaaca gcuggcuacg aagauuagac ucuggcacca gcagggagag     1740

cgcugccugu acaccggcaa aacaaucagc auccaugacc ugauuaacaa uuccaaccaa     1800

uuugaaaucg aucacauucu uccccucucu aucaccuucg acgauagccu ggccaauaag     1860

guccuggugu augcgacagc caaccaggag aaagggcagc ggaccccuua ccaagcucuc     1920

gacuccaugg augacgccug gagcuuuaga gaacugaagg cuuucguucg cgauucuaag     1980

gcccuuucca auaaaaagaa agaguaccug cucacggaag aggacauuag caaguuugau     2040

gugcggaaaa aguucaucga gagaaaccug gucgacaccc gguaugcguc ucgcgugguc     2100

cugaaugccc uucaggaaca uuuuagagug cacaagacag acaccaaagu uuccgugguc     2160

cggggacagu ucacaagcca gcucagacgc cauuggggca ucgagaagac ccgggauacg     2220

uaccaccacc augcugugga cgcccugauu aucgcugccu ccucucaacu gaaccucugg     2280

aaaaagcaga aaaauacacu gguuagcuau uccgaagauc agcuucugga cauugagacc     2340

ggggagcuca ucucugauga cgaauacaag gagagcgugu uuaaggcccc cuaucagcac     2400

uucgucgaua cacugaaauc caaggaguuc gaagacagca uucuguuuuc uuaccaagug     2460

gauuccaaau ucaacagaaa gaucagcgac gcuaccaucu augcgacccg ccaggccaaa     2520

gugggcaagg auaaaaagga cgaaacauac guucuuggaa agauuaaaga uaucuacucu     2580

cagacgggcu augacgcuuu uauuaagauc uacaaaaagg acaaauccaa guuccucaug     2640

uauagacaug auccgcagac cuuugagaag gucauugagc cuauccugga aaauuacccc     2700

aacaaagagc ugaaugaaaa ggggaaagag gugccuugua accccuuccu caaguauaaa     2760

gaagaccacg gauacauucg gaaguauagc aagaaaggca acgggcccga gaucaagucu     2820

cugaaauacu acgauagcaa acuuggaaau cacaucgaca uuacaccuaa gaacuccaau     2880

aacaaggugg uccugcaaag cgugucuccg uggcgcgccg auguuuauuu uaauaagacc     2940

acaggcaaau acgagauccu cgggcugaag uaugccgacc ugaaauucga aaagggcacc     3000

ggaacguaca aaauuuccga ggaaaaguau aacgauauca agauuaaaga gggcguggac     3060

agcgauuccg aauuuaaguu cacacuuuac aaaaaugacc uccugcugau caaggauacc     3120

gagaccaaag agcagcagcu cuuuagauuc cugucucgga caaugcccaa cgucaagcau     3180

uauguggaac uuaaaccuua cgacaagcaa aaguuugacg auaaugagga acugaucaaa     3240

auucucggga ucguugcuaa gggaggccag ugcaaaaagg gggugagcaa acccaacauu     3300

uccaucuaca agauucgcac cgacguccug ggaaaucagc acaucaucaa gaacgagggc     3360

gauaaaccua agcuggacuu cuga                                            3384


<210>  3966
<211>  3384
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3966
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaggauu ccggaaaaau uauccacgcu aauucuagaa uuuuccccgc cgcgaccgcu      120

gacaacaaug uggaacggcg cacaagcaga caggcccggc gccugaacag acggaagaaa      180

caucgcuccg ugagacucca cgaucuguuu gaggaauacg gccuucugac ggacuucucu      240

aaggucagca ucaaucucaa cccuuaucaa auccgggugc aggggcugga uaaacgccug      300

accaaugagg aacucuuuau ugcccugaag aacaucguua agagacgggg aauuuccuac      360

cuugacgaug cuucugagga cggcggcgcc gugagcuccg auuaugggaa agcugucgag      420

gaaaaucgca agcugcucgc ccagcagaca ccgggacaaa uccagcugga cagauucgag      480

aaauacggcc aggugcgggg agauuuuacc guuguggaaa acggagagaa gcgcagacuu      540

auuaaugucu ucagcacauc ugcguaucgg aaagaggccg aaagaauccu gcgcaagcag      600

caagaguuua acaauaagau uaccgaaggc uucaucgagg acuaccucac aauccugacc      660

gggaaacgga aguauuacca uggacccggc aacgaaaaau ccagaacgga uuacggccgc      720

uauaccacaa agaaagacag ccagggggag uacauuaccc uggacaauau cuuugggauu      780

cucaucggca agugcacauu cuauccugau gaguaccggg cuucuaaagc cuccuauacc      840

gcucaggaau uuaaccugcu uaacgaccug aauaaccuca cggugcccac agagaccaag      900

aagcugagcg uggaacagaa aaagacaauu gucgaguacg ccaaaaccgc gaagacccuu      960

ggggccucua cacugcucaa auauaucgcu aagcugaucg gaguuuccgu ggaucaaauu     1020

cacggcuaca gaaucgaccc uaaugaaaag cccgagaugc auacguucga gguguaccgc     1080

aaaaugcaga gccuggaaac cauucggguc gaggaacucc cuagaaaggu gaucgaugag     1140

cuggcccaca uucuuacacu gaacaccgaa cgcgagggga ucgaggaagc uaucaaugcc     1200

aaacucaagg acaucuuuuc ccaggaucaa guucuggagc uugugcaguu ccggaaaaac     1260

aauucuagcc uguucuccaa gggauggcac aacuuuagcc ucaagcugau gauggaacug     1320

auuccggagc ucuaugagac aucugaagaa cagaugacca uucugacgag acuuggcaaa     1380

cagaagucca aagaaacaag caagcgcacc aaauacaucg acgagaagga acugaccgag     1440

gagauuuaua aucccgucgu ggccaagucu guucggcaag cuaucaaaau uaucaacgaa     1500

gcgacaaaga gauacggcau cuucgauaau auugugaucg agauggcccg ggaaaacaau     1560

gaggaagacg cuaaaaagga guauauuaaa cgccagaagg ccaaccucga ugagaaaaau     1620

gccgcuaugg aaaaggccgc guuucaguac aacgggaaga aagagcugcc ugacaacguc     1680

uuccauggac acaaggaacu ggcuaccaaa aucagacuuu ggcaucagca aggcgagaag     1740

ugucucuaua cagggaaaaa uauucccauc agcgaccuga uccagaaccg guacaaguac     1800

gaaauugauc acauccugcc ucucucccug ucuuuugacg auagccuuuc caauaaggug     1860

cugguccucg ccacggccaa ccaggagaaa ggacagagaa cccccuucca agcucuggac     1920

agcauggauu augccugguc uuaccgcgag uuuaaguccu augugaaaga cagcaagcug     1980

cuuucuaaua aaaagaagga uuaccuccug acagaagagg acauuuccaa aaucgaaguu     2040

aagcagaaau ucauugagcg gaaccuggug gauaccagau auagcucccg cgucgugcuc     2100

aaugcucugc aggacuuuua caaggagcac caguucgaua caaccaucuc uguugugaga     2160

ggccaauuua cgagccagcu ucggcgcaaa ugggggaucg aaaaguccag agagaccuau     2220

caucaccaug ccgucgacgc gcugauuauc gccgcuucua gccagcuccg gcuguggaag     2280

aaacacaaca auccgcugau uuccuacaag gaggagcaau ucguggauag cgaaacaggc     2340

gagaucauuu cuauguccga cgacgaguac aaagaacuug uguuuaaggc cccuuaugau     2400

cauuucguug acacccucaa aagcaagaaa uucgaggauu cuauccuguu uuccuaccag     2460

gucgacagca aguauaaccg caagaucucu gaugcuacaa uuuacgccac cagaaaagcg     2520

aagcuggaca aagauaagag cgaagagacg uaugugcucg gaaaaaucaa ggacauuuac     2580

ucccaggccg gcuaugauac auucaucaag auuuacaaua aagacaagag ccgguuucug     2640

auguaccaca aagaucccca gaccuucgaa aaggugaucg aggagauucu ucgcacauau     2700

ccuucuaaag aacugaacga gaagaauaag gaaguccccu gcaaccccuu ugagaaauac     2760

agacaagaaa augggccuau ccggaaguau ucccgcaaag gaaacggccc ggagaucaag     2820

agccucaaau acuaugacaa caagcugggg aaucacauug auaucacccc caacgagucc     2880

augaaucagg ugguucugca gucucuuaag ccuuggagaa ccgacguguu cuuuaaccau     2940

cagacagaaa aauacgagcu caugggacug aaguauagcg accugaauuu cgaaaaaggc     3000

uccgggaagu acucuauuag ccucgagaaa uacaacucca ucaagaugaa ugagggcguc     3060

ucugaagaga gcgaauuuaa guucacgcug uauaaaaacg aucuuauucu gaucaaggac     3120

gcucucacca aagaggccca aaucuuucgg uacggaucca gaaaugauac aagcaagcac     3180

uauguggaac ugaaacccua cgacaugucu aaguucgagg gcgaugagcg ccugauuaaa     3240

guucuuggga ccguggcuaa gggaggccgg uguaucaagg ggcugaacaa aaaggacauu     3300

uccaucuuua aagucaagac agaugugcuc ggaaauaaac auuucauuaa gaaggaaggc     3360

gaggaaccua aacugaaguu cuga                                            3384


<210>  3967
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3967
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uuggcagcaa cuccaucggg       60

uucgccgcug ucgaugaaaa uucucaccuc auuagacuga agggaaaaac aguuaucggc      120

gcccggcuuu uugaagaggg gaaggcggcu gccaaacgca gagccggacg gacgacccgc      180

agacggcuga gccgcaacag auggcggcuc uccuuccugc gcgacuuuuu cgaaucucau      240

auuacaccua ccgauccgaa cuuuuucaug agacagaagu auagcgagau cucccccaag      300

gacaaagcuc gguacaagua ugaaaaacgc cuuuuuaaug auagaacaga cgccgaguuc      360

uaccaacagu auucuaccau guaccaucug cggaaucgcc ucaugacaga uccuagcaga      420

gcugacgugc gggagaucua uuuugccauu caccacaucc ugaaguccag aggccauuuc      480

cugacccccg gcgaugcgaa agacuuuaac acgaaugaug uggcccucga cgaaauuuuc      540

ccugcucugc aggaugccua cgcucagguc uaccccgacc uugggaucac cuuugacgag      600

gauaaggcca acgaguucaa gacaauucug cucaaugaga ucgcgaccag cauugacaca      660

caacgcgccc uggugaaacu ucugcucgcu gaggaagaug acaaggauau ccugaaacag      720

cagaagcagg uucugaccga guucgccaaa gcuguggucg gacucaagac gaaacugaac      780

cuugcccugg gcacagaagu ggacucuucc gaggccaccg cuuggaauuu uagccucggg      840

caacuggaug acaagugggc gggaaucgaa ucugccauga cagaugaggg caccgagauu      900

cuugaccaga uccgggaucu guauagagcu cgccuccuga acgggauugu uccuaccgga      960

aagacacugu cccaggccaa aguggacgac uacacgcagc accacgaaga ucuccaacug     1020

uucaaggccu aucuuaaaca gcuggaggac gauggcaccg cuaaggccau ccggcagcuc     1080

uacgacagau auauugaugg cgacgaugcg gcuccguuuc ugcgcgaaaa uuucgucaaa     1140

gcccuuacaa aggacgugac cgcccauccc aacacaaaga gcccugagcu gcucgaacgg     1200

cuggcucaac ccgauuuuau gcugaaacag agaaauaagg ccaacggggc uaucccugug     1260

cagaugcagc aacgcgagcu cgaccagauc auuaaaaacc aggccgucua cuaugauugg     1320

cuggcggccc ccaauccggu ugagaagcac cggaaaucua ugccuuacca gcuugacgaa     1380

cugcucaacu ucagaauccc cuacuaugug ggaccucugg ugaccgcuaa ggagcaaaag     1440

gccgcucagg gcggggucuu ugccuggaug gugcgcaaag aucccgaagg aaauauuacg     1500

cccuacaacu ucgacgagaa gguugaccgg gaagcguccg ccaauacauu uauccagaga     1560

augaaaacca ccgauacaua ucugauuggc gaggacgugc uuccuaagca gagccuccug     1620

uaccaacggu augagguccu gaacgaacuc aauaacgugc gcguuaauaa cgagaaacug     1680

uccaucgaac agaagcaaca ggugaucaga gagcuuuucg agcggcauaa uucugucacc     1740

auuaagcaau uugcugaaaa ccugagagcc cacggggauu acgcucacau cccggagauu     1800

cgcggacucg ccgacgaaaa acgguuccug agcucccuga gcacauauag acagcuuaag     1860

ucucuccugc ccgaggccau cgaugacccu gcuaaacagg cggauauuga aaauaucauu     1920

gccuggucca cgguguuuga ggacgcugcc aucuucaaga ccaaacugaa ggagaucagc     1980

uggcucggcu cugaagccau uacaaaacug agcaacaucc gcuacagagg cugggggcaa     2040

uucucccgga aguuucuuaa uggacugacc cucggcaacg ggcauacaau uauccaggag     2100

cugcugcuuu cuaccaacaa ucucaugcag auucugacgg augaaacccu gcagaagaaa     2160

augacagagc ucaacgcuga caagcugaaa accgccaaua ucaacgaugc gaucgacaau     2220

gcuuacacaa gccccuccaa caagaaagcc cuucgccaag uccugagagu gguugaugac     2280

auuaagcggg ccgcugacgg acaggauccu agcuggcucu auguggaaac cgccgacggc     2340

gggggcacgc ccggaaagcg cacaagagcg cggcagcacc agcugcaaga gaucuacgcu     2400

aaugccgccc augagcugau ugauaccgcu guccgcggcg aacuugagga caaaaucucu     2460

gauaaggccg acuucaacga uagacucgug cuguauuuua ugcaaggggg acgggacauu     2520

uacacaggcg cuccgcugaa uaucgaucag cucuccagcu augacaucga ucacauucug     2580

ccucagucuc uuaucaaaga caacucccug gacaaucgcg uucucgugca ugccauuauc     2640

aacagagaaa agaaugcgac cuucgccagc accauuuacg cucagaaaau gaacgccaca     2700

uggcggcagu ggcacgaggc ugggcugauc uccggaagaa agcugcgcaa ucuucagaug     2760

cggcccgauc agaucgacaa guaugccucu ggcuuugucg ccagacagcu gguggaaacg     2820

cgccagauua ucaaacucac cgagcagauu guggcugcgc aauacccuga uacaaagauc     2880

auugccguua aagcugggcu gagccaccag cuccgggagg aacuggaguu ccccaagaac     2940

agagacguca accauuacca ccaugccuuu gaugccuucc uugcugcccg caucggcacc     3000

uaucugcuca aacgguuucc uaaucugcag gcguucuuua cauacggaaa guucaccaag     3060

gcugacguga aaaagcugag aggcuuuaac uucauucgcg auaucacgca cgccgaagac     3120

aaaaucgugg ccaaggauac aggggagguc gugugggaca aacagcggga uguugacgag     3180

cuugauagaa uuuauaauuu caagcggaug cugaucaccc acgaagugcg cuuugagacc     3240

gcugaccucu ucaaacaaac agucuacgga gccagagacu ccaaggaagc gggcgggucu     3300

aagcagcuga uucccaaaaa gaaagauauc cagcucauuu uuaugggagc uaucagcgag     3360

cggacccgcc cgauccugcc ucuuuga                                         3387


<210>  3968
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3968
auggacgaga agauggauua cagaaucggc cuggacauug ggaucgccag cgugggaugg       60

gcuguccucc agaacaauuc cgaugacgaa cccguucgga uuguggaucu gggcgugcgc      120

aucuucgaca ccgccgagau uccuaaaaca ggggaaucuc uugcgggacc gagacgggcu      180

gcccgcacga ccagacggcg ccugagacgg cgcaagcaca gacucgaucg gaucaaaugg      240

cuguuugaga accaaggccu uaucaauauu gacgauuucc ugaagcgcua uaacauggcc      300

gggcuccccg acgucuacca gcugagauau gaagcucugg aucggaaacu cacagacgag      360

gagcuggccc aggugcuucu gcauaucgcu aagcaccgcg gauuuagaag cacccggaag      420

gccgaaacag cggccaaaga gaauggcgcu guucucaagg ccaccgauga aaaccagaaa      480

agaaugcaag agaagggcua ccgcacagug ggggagauga uuuaucugga cgaggcuuuc      540

cggaucggau gcuccugguc ugagaaaggc uacauucuua ccccuagaaa uaaggccgaa      600

aacuaucagc auacgaugcu gcgcgcgaug cucgucgagg aagugaagga gaucuuuagc      660

ucccagcgga gacuggggaa uaaaaaggcc accgaggaac uggaggaaaa auaccucgag      720

auuaugacau cucagcgcag cuucgaccug ggacccggca ugcaaccuga ugggaagccc      780

uccccuuacg cuauggaagg auuuagcgac cggguuggca aauguaccuu ccuuggcgau      840

cagggggagc ugagaggagc caagggcaca uauaccgcug aguacuuugu ggcccuccag      900

aaaaucaacc acacgaagcu ggucaaucag gacggggaaa cacgcaacuu caccgaggaa      960

gaacggagag cccuuacacu gcuccuguuu acccaaaagg aagugaaaua ugcugcggug     1020

cgcaagaaac ugggacuccc ggaggauauc cuguucuaca aucuuaacua uaagaaagcc     1080

gcuaccaagg aggaacagca gaaggagaau caaaacacag aaaaagccaa guuuauuggc     1140

augcccuacu aucaugacua caaaaagugc cuggaggagc gggucaaaua ccucacggaa     1200

aacgagguua gagaucuguu cgacgaaauc gggaugauuc uuaccuguua uaagaaugau     1260

gacucucgga cagagcgccu ggccaagcuc ggacuggugc cuaucgaaau ggagggccug     1320

cucgcuuaca cccccacaaa auuccagcac cuguccauga aggccaugag aaacauuauc     1380

ccuuuucuug agaaaggcau gaccuaugau aaggcgugcg aagaggcugg guacgacuuc     1440

aaagccgaca gcaagggaac gaagcagaaa cugcucacag gcgaaaaugu gaaccagacc     1500

aucaaugaga uuaccaaccc cgucgugaag cggucuguuu cccaaacagu gaaagucauc     1560

aaugccauua ucagaaccua ugggagcccg caggcuauua acaucgaacu ggcccgcgag     1620

augucuaaga cauuugagga acggagaaaa aucaagggag auauggagaa acgccagaag     1680

aauaacgaag acgugaagaa acagauucaa gagcugggca aacuuucccc uacggggcag     1740

gauauccuca aguacagacu guggcaggaa cagcaaggca uuuguaugua uagcggaaag     1800

accauccccc uggaggaacu cuucaaaccu ggcuacgaca uugaucacau ccugcccuac     1860

uccauuacau uugacgauuc uuuccggaau aagguucuug ugaccagcca ggaaaaccgc     1920

cagaagggga auagaacacc cuaugaguac augggaaacg acgaacagcg guggaaugag     1980

uuugagaccc gcgucaaaac gaccaucaga gauuauaaga aacaacagaa gcugcucaaa     2040

aagcauuucu ccgaagagga acggagcgag uuuaaggaac gcaaccugac agacaccaaa     2100

uacaucacaa ccgugauuua uaacaugauc agacagaauc uggagauggc uccucuuaac     2160

cggccggaga agaaaaagca aguccgcgcc gugaauggcg cgauuacggc cuaccucaga     2220

aaacgguggg ggcugcccca gaagaacaga gaaacagaua cccaccaugc uauggacgcc     2280

guugugaucg cuugcuguac agauggaaug auucagaaga ucucucgcua uaccaaaguc     2340

cgggagagau gcuacuccaa gggcaccgag uucguggacg ccgagacagg ggaaaucuuu     2400

cgcccugagg acuacagccg ggcggagugg gaugaaauuu ucggcguuca cauccccaaa     2460

ccuugggaga cguuuagagc cgaacuggac gugcgcaugg gagaugaccc caagggcuuc     2520

cucgauaccc acucugacgu cgcucuggag cuugauuauc cggaauacau uuaugagaau     2580

cugcggccua ucuucgugag cagaaugccc aaccauaaag ugacaggggc cgcucacgcc     2640

gacaccauuc gcuccccucg gcauuuuaag gaugagggaa ucguucucac aaaaaccgcc     2700

cugacggacc ugaaacuuga uaaggacggc gaaaucgaug gguacuauaa uccccagucu     2760

gaccuccugc uguacgaggc ucucaagaag caacugcuuc uguauggaaa cgacgcgaaa     2820

aaggccuucg cucaggauuu ucacaaaccu aaggccgacg gcacagaagg gcccgucgug     2880

agaaagguga aaauucagaa gaaacagacc augggcgucu ucguggauag cggaaauggc     2940

aucgccgaga acgggggaau gguucggauu gacguguuuc gcgucaaugg caaguacuac     3000

uucgugccgg uuuauaccgc ugaugugguc aaaaaggugc ucccuaacag agccuccaca     3060

gcgaauaagc ccuacagcga guggcggacc auggaagaca aagauuuucu guucucucug     3120

uauucccgcg accuuguuca uaucaagagc aaaaagggga uucccaucaa aaacguggau     3180

ggaucucucg gcgaguccaa ggaagucuac gcuuauuaca ucggggccga cauuagcggc     3240

gccaagaucg agggaaaagc ucacgauucc agauauacau uucggggcau uggggugcaa     3300

aagcuggaac ugaucgagaa augucaggug gacauucucg gacacaucuc uacggucaag     3360

aaugagaaaa gaaugggcuu cagcuga                                         3387


<210>  3969
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3969
augaacgaca agaaucagaa auacagacug ggccucgauc uggggaccaa cagcaucgga       60

uggugcauuu auucccuuga cgugaauaac aagcccgaga cacugaucga uaugggcguc      120

cggauuuucu cugacgggcg cgauccuaaa acgaaggaac cgcucgccgu ugagagacgg      180

accgcucgcg gacugagacg gauccuucac cgcagaaaac ugcggcgcag acaaauguuu      240

cggcuccugc aggaacaggg ccugaugccc aaugacgcca gcgaggcggc ugcccucaag      300

ucccugaacc cuuacgaacu ucgcgugaag gcccuggaua caaaacucga gcccuaugag      360

cuggggagag cucuuuucaa ucugucugug cggcgcggau uuaagagcaa cagaaaagaa      420

gccguccagg agaaggaaaa agacucccaa aaugaugcua agucucagaa cgagaagugu      480

gagcaucucg ccaaagcgau uaaggacagc ggcugccgga cccugggcga guuccugugg      540

aaacacaaga auacagagac ccagcagaca gaaaacgaug ccagaaauau cgggaugcgc      600

uuugugccug gacgguccaa cuacuauccc accagacgca uguacguuga ggaguucgac      660

gcuauccgga aagcccaaga accuuauuac agcggcguga auugggaugc ucuccaugag      720

aagcuguuuu accagagacc gcuucgcgcc caggaacggg ggaaauguag auauaugccc      780

gacaaggagc gcacguucaa ggcgaugccu ugcucucaga aaauucggau ccugcaagaa      840

gucuacaaca uggacuauau ugaugaguac ggaaagacca ucccccucga agaggcccag      900

acagacuccc ugauuagccu ucuggauaaa agagagaagc ucucuuuuaa agcuaugaag      960

aaggaacugg gcaucgacga guccuguacc uucaaccugg aacgcggggg acgggaguau     1020

cuccagggca augccgcugc cgugaaacug agaaacaaga aacgguuugg cgaacuuugg     1080

gauagccugu cucucgccaa gcaagacgcu auuguugaga aacugaucac agcggaugag     1140

gacgccgaaa ucauuuccau gcuugcucag uacaaucuga ccgaugagca gaagaagaac     1200

aucgccguga cgguccuccc uagcgggaca gccaugcugu gcaaagaagu gaccgagaug     1260

cuggugcaga agauggagca aaauaaaauu ccccucacag ucgcuguuca gaugcuggga     1320

uauaccuacg ccgaccaguc cgugcgcgaa gaggauaccc uuccguacua uggcgcgauc     1380

cugacagggu cuacgauggg agcucacccu gaagccgacg agagcgaacc cgagcucaag     1440

uacggcaaaa uuuccaaccc uaccgugcau gucgcccuga aucagacaag aaccgugguu     1500

aacgcucuga ucaaggccua ugggaagccc caacagaucg uggucgagcu uagccgggac     1560

cucaaggcuu cuagagaagc caaagcgcgc auucagaaaa caaucaccau gaaucagaag     1620

cggaacgaga gagauaauaa aaacauuacg gacaucacaa agauucccua cccuaaucgc     1680

aacgauagac ugaaauaucg gcugugggaa gaacucggau ccggcagcaa uucuuucagc     1740

cgcaaguguc uguacugcgg caagccgauc uccgggucug aaaucuuuac cgagaacauu     1800

gagaucgaac acauucuucc cuucggaaga acccuguucg acggcgagac aaacaaaacc     1860

guggcccaca cagauuguaa ucgggacaag ggggaucgca gcccuuuuga ggcuuucgga     1920

uccagcccca acggcuauaa uuggcaugaa aucugcgcca gagcuaacuc ucucaaaaau     1980

ccugccaagc ggacgcuguu ugcggaaaac gccauggaga aauucgagaa ggacaauucc     2040

uuuauucaac gccagcugac cgauaacgcu uaccuuagca gaucugcccu ccgguaccug     2100

cgcuguaucu gcaaggacgu uugguccauu aaugggggca ugacaaaacu gcucagagau     2160

aaguggaaca ucgacagcau ccugaaacgg aagauugaug accccgaaau cgcucacuuc     2220

gagcuuaaaa augaccagau uggacaguau aagaagaaca gauacgauca ucgccaccac     2280

gcccuggacg ccuccgugau cgcucucauu gaucgggcga ugguccaaga aaucucucag     2340

cugaauaaaa ucagacugaa gaaccgcauu gaggccccgc agaugccugu gcuucggagc     2400

gagcucaucg aaaaagucaa gcauauuacc guguccuuua aacccgacca cggcagacaa     2460

gggaagcugu cuaaggagac acugcucgga aaaaucaaga aaauugaaaa gguugauauc     2520

gacagcaucc agaccgauga cauugcuucc aucaaagaug acgccauucg cacgcgguuc     2580

gagaccaaga gacgcgaacu gggcgauauc acaaaagcca aggcugcccu uaagaccgag     2640

uuuccuaaac uggacguguu caagacauau uacgucagcc ggacccccau ugugucucuc     2700

acggagaaaa auaucaacuc caucguugau gaaaagauua aagagaagcu gaagacauuu     2760

aucaaagaac augcggaccu gagcuucgcu gagcagcuuc agaaguuuuc ugaaaccgag     2820

uggauuggga aaccuuccgc cgccggaaag cccggcaaaa aguauagaau cgagaaggug     2880

cggugcauua acugggggca aacaccgauc accauuacca gcucugcugu cccucgcuac     2940

cucugucccg aagacuaucu ggccgcggug guguggcaga uccccagcaa aaagaaagau     3000

acagagccug aauacaaggc uacguuccug agacgggacg aguucgaugu uaauaacaaa     3060

ccgaagaagc ucgaaaaacc ccacgacgcc gcccaguaca ucugcaugcu guauaaggau     3120

gacuaccuug aguuuaccga guccggcgcu acacaucugu gucgcauugc cggacucccu     3180

gcuagccaga auaacaaaau cgauauuaga cccaucuaug ccgucucuga cugcaaggau     3240

uggauuaucg cgaccaauga aaacaugcug gagccuuguu ggaaacccca aaaggaagag     3300

aagcggaucu ccgugaaugu gcuguucggc aaacacaagg ccagaagcgu cacagugaac     3360

ccuauugggc gcguuuuucg gaaauga                                         3387


<210>  3970
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3970
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaaug uggagcggcg cacaaacaga caaggccggc gccugacgag acggaagaaa      180

caucgcagag uccggcucaa ucaccuguuu gaagagagcg ggcuuaucac cgacuucaca      240

aaggugucca ucaaccugaa uccuuaccag cuccgcguua aaggacugau ugaugaacug      300

ucuaacgagg agcucuuuau cgcgcugaag aauaugguga agcauagagg cauuagcuau      360

cuugacgaug cuuccgacga uggcaacucu agcgucgggg acuacgccca gaucgugaaa      420

gaaaauucca agcagcugga gaccaaaaca ccgggacaaa uucagcucga acgguaucag      480

aaguacggcc agcugcgcgg ggauuucacc guugaggagg acggaaagaa gcacagacuu      540

aucaacgugu uucccacaag cgccuaucgg ucugaagcuc ugcgcauucu ccaaacccag      600

aaggaguuca auccucagau cacggaugaa uuuaucaaca gauaccugga gauucugacc      660

ggcaaacgga aguauuacca ugggcccgga aaugaaaaau ccagaacaga cuacggccgc      720

uauaccacaa agaaagauag cgagaacgag uacaucaccc ucgacaacau uuucggcauc      780

cugauuggga agugcacguu uuauccugaa gaguaccggg ccgcuaaagc cucuuauaca      840

gcgcaggagu ucaaucuucu gaacgaccuc aauaaccuga ccguccccac agaaaccaag      900

aaacuuuccg aggaacagaa aaaucagauc aucaacuacg ugaagaauga gaaagccaug      960

ggaccugcua agcuguuuaa auauauugcc aagcuccuga gcugugaugu ggcugacauc     1020

aagggcuaca gaauugauaa aucugacaag gccgaaaucc acaccuucga ggcguaccgc     1080

aaaaugaaga cacuggaaac gauugauuuu gagaaaaugu cccgggacca gcucgauaag     1140

cuggccuaug uccuuacccu gaacacagaa agagagggga uccaagaggc ucucgaccac     1200

gaguucgccg auggaaauuu cagccaggaa cagaucgacg aacugguuca guuucgcaag     1260

gcuaacuccu cuauuuucgg caaagggugg cauaauuuua gcgugaaacu uaugauggag     1320

cugaucccgg agcucuacgc caccuccgag gaacaaauga caauucugac ccggcuggga     1380

aaacagaaga cgacaucuag cuccaacaaa accaaguaua ucgaugaaaa gcuccugacc     1440

gaggaaauuu acaaucccgu ggucgccaaa agcgugagac aggcuaucaa gauuguuaac     1500

gcggccauca aagaguaugg cgacuucgac aauaucguga uugagauggc ucgcgaaaca     1560

aacgaggaug acgaaaagaa agccauccag aagauucaaa aagccaacaa ggaugagaag     1620

gacgcugcca ugcuuaaagc ggcuaaucag uacaacggcc gggccgaacu gccucacucu     1680

gucuuucaug ggcacaagca gcucgccacc aaaaucagac uguggcacca gcaaggagag     1740

cgcugccugu auacaggcaa gacgauuagc auccaugauc uuaucaauaa ccccaaucag     1800

uucgagauug accacauccu cccucugucc auuaccuuug augacucucu ggcuaacaaa     1860

gugcucguuu acgccacagc uaaucaggaa aaggggcagc ggacccccua ccaagcccug     1920

gauagcaugg acgaugcgug guccuucaga gagcuuaagg ccuuugugcg ggaaagcaaa     1980

ucucugucca acaagaaaaa ggaguaucuc cugacagaag aggacaucag ccgcuucgau     2040

gucagaaaga aguuuauuga gcggaaucug guggacacca gauacgcuuc ucgcgucgug     2100

cuuaacgccc uccaggaaca uuuccgggcu cacaagacgg acaccaaagu uuccgugguc     2160

agaggacagu uuacaagcca acugcgcaga cauuggggca ucgagaagac ccgggauaca     2220

uaucaccacc augccgugga cgcgcugauc auugccgcuu ccucucagcu caaucugugg     2280

aaaaagcaga aaaacacccu uguuaauuac agcgaaaacc agcugcucga uaucgagacg     2340

ggggagcuga uuuccgacga ugaauauaag gagucugugu ucaaggcccc guaccaacac     2400

uucgucgaca cacugaaaag caaggaauuu gaggauucca uccuuuucag cuaucaggug     2460

gacucuaaau uuaaucgcaa gauuuccgau gcuaccaucu acgccacaag acaggccaaa     2520

gugggcaagg acaaaaagga ugaaaccuac guucucggaa agaucaaaga cauuuauagc     2580

cagaccggcu acgaugcuuu caucaagauu uaucaaaaag acaagucuaa auuucugaug     2640

uaccggcaug acccucagac auucgagaag gucaucgagc ccauucugga aaacuauccu     2700

aacaaggaga ugaaugaaaa agggaaggag gugcccugua accccuuucu caaauacaag     2760

gaggaacacg gagauuauau ccgcaaauac uccaagaagg gcaaugggcc ugagauuaaa     2820

agccugaagu acuaugacuc uaaacuugga aaccacaucg auaucacgcc gaaggacagc     2880

aauaacaaag ugguccugca guccgugagc cccuggagag cggauguuua cuucaauaag     2940

accacaggca aguaugaaau ucucgggcug aaauacgccg accugcaguu ugagaagggc     3000

accggaacau auaaaaucuc ucaagaaaag uacaacguga uuaaaaagaa ggagggcguc     3060

gauuccgaca gcgaguucaa auuuacccuu uauaagaaug aucuccugcu gaucaaagac     3120

acggaaacaa aggagcagca acucuuccgg uuucuguccc gcaccaaacc uaacgugaag     3180

cauuacguug aacuuaaacc cuacgauaag caaaaguucg aggggaauga aucucugauu     3240

aacgugcucg gagcugucgc caaaggcggg cagugccaga agggaaucaa uaaaccuaac     3300

aucagcauuu auaaggugag aaccgacguu cugggcaauc agcacaucau uaaaaacgag     3360

ggggacaagc ccaagcugga uuucuga                                         3387


<210>  3971
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3971
auggacgaga agauggauua cagaaucggc cuggacauug ggaucgccag cgugggaugg       60

gcuguccucc agaacaauuc cgaugacgaa cccguucgga uuguggaucu gggcgugcgc      120

aucuucgaca ccgccgagau uccuaaaaca ggggaaucuc uugcgggacc gagacgggcu      180

gcccgcacga ccagacggcg ccugagacgg cgcaagcaca gacucgaucg gaucaaaugg      240

cuguuugaga accaaggccu uaucaauauu gacgauuucc ugaagcgcua uaacauggcc      300

gggcuccccg acgucuacca gcugagauau gaagcucugg aucggaaacu cacagacgag      360

gagcuggccc aggugcuucu gcauaucgcu aagcaccgcg gauuuagaag cacccggaag      420

gccgaaacag cggccaaaga gaauggcgcu guucucaagg ccaccgauga aaaccagaaa      480

agaaugcaag agaagggcua ccgcacagug ggggagauga uuuaucugga cgaggcuuuc      540

cggaccggau gcuccugguc ugagaaaggc uacauccuua cgccuagaaa uaaggccgaa      600

aacuaucagc auaccaugcu gcgcgcgaug cucgucgagg aagugaagga gauuuuuagc      660

ucccagcgga gacuggggaa ugagaaagcc acagaagaac uggaagagaa guaccucgaa      720

aucaugaccu cucagcgcag cuucgaccug ggacccggca ugcaaccuga ugggaaaccc      780

uccccuuacg cuauggaggg auuuagcgac cggguuggca aguguacauu ccuuggcgau      840

cagggggagc ugagaggagc caaaggcacc uauacggcug aauacuuugu ggcccuccag      900

aagauuaacc acacaaaacu ggucaaucag gacggggaga cccgcaacuu cacagaagag      960

gaacggagag cccuuacccu gcuccuguuu acccaaaagg aggugaagua ugcugcggug     1020

cgcaaaaagc ugggacuccc ggaggauauc cuguucuaca aucuuaacua uaaaaaggcc     1080

gcuacaaaag aagaacagca gaaggaaaau caaaacacgg agaaggccaa auuuaucggc     1140

augcccuacu aucaugacua caagaaaugc cuggaggaac gggucaagua ccucaccgag     1200

aacgaaguua gagaucuguu cgacgagauu gggacaaucc uuaccuguua uaaaaaugau     1260

gacucucgga cagaacgccu ggccaagcuc ggacuggugc cuauugagau ggagggccug     1320

cucgcuuaca cccccacgaa guuccagcac cuguccauga aagccaugag aaacaucauu     1380

ccuuuucuug aaaagggcau gacauaugau aaagcgugcg aggaagcugg guacgacuuc     1440

aaggccgaca gcaaaggaau caagcagaag cugcucaccg gcgagaaagu gaaucagacc     1500

aucaacgaaa uuacaaaucc cgucgugaag cggucuguuu cccaaaccgu gaaagucauc     1560

aacgccauua ucagaacaua ugggagcccg caggcuauua auaucgagcu ggcccgcgag     1620

augucuaaga cguuugagga gcggagaaaa aucaagggag auauggaaaa acgccagaag     1680

aacaaugagg acgugaagaa acagauucaa gaacugggca aacuuucccc uaccgggcag     1740

gauauccuca aguacagacu guggcaggag cagcaaggca uuuguaugua uagcggaaag     1800

acaauccccc uggaggaacu cuucaaaccu ggcuacgaca uugaucacau ccugcccuac     1860

uccauuaccu uugacgauuc uuuccggaac aagguucuug ugacaagcca ggagaaucgc     1920

cagaagggga acagaacccc cuaugaauac augggaaaug acgagcagcg guggaacgag     1980

uuugaaacgc gcgucaaaac cacaaucaga gauuauaaga aacaacagaa gcugcucaaa     2040

aagcauuucu ccgaggagga acggagcgaa uuuaaggagc gcaaucugac cgacacaaaa     2100

uacaucacca cggugauuua uaacaugauc agacagaacc uggagauggc uccucuuaau     2160

cggccggaaa agaaaaagca aguccgcgcc gugaacggcg cgauuacagc cuaccucaga     2220

aaacgguggg ggcugcccca gaagaauaga gagaccgaua cacaccaugc uauggacgcc     2280

guugugaucg cuugcuguac cgauggaaug auucagaaga ucucucgcua uaccaaaguc     2340

cgggaaagau gcuacuccaa gggcacagag uucguggacg ccgaaacggg ggagaucuuu     2400

cgcccugagg acuacagccg ggcggaaugg gaugagauuu ucggcguuca cauccccaaa     2460

ccuugggaaa ccuuuagagc cgagcuggac gugcgcaugg gagaugaccc caagggcuuc     2520

cucgauacac acucugacgu cgcucuggaa cuugauuauc cggaguacau uuaugagaac     2580

cugcggccua ucuucgugag cagaaugccc aaucauaaag ugaccggggc cgcucacgcc     2640

gacacaauuc gcuccccucg gcauuuuaag gaugaaggaa ucguucucac caaaacggcc     2700

cugacagacc ugaaacuuga uaaggacggc gagaucgaug gguacuauaa cccccagucu     2760

gaccuccugc uguacgaagc ucucaagaag caacugcuuc uguauggaaa ugacgcgaaa     2820

aaggccuucg cucaggauuu ucacaaaccu aaggccgacg gcaccgaggg gcccgucgug     2880

agaaagguga aaauucagaa gaaacagacc augggcgucu ucguggauag cggaaacggc     2940

aucgccgaga augggggaau gguucggauu gacguguuuc augucaacgg caaguacuac     3000

uucgugccgg uuuauacagc ugaugugguc aaaaaggugc ucccuaaucg cgccgcgacc     3060

gcucacaagc ccuacgggga auggaaaguu auggaggaca aggauuuucu guucucccug     3120

uauagcagag accuuaucca cauuaaaucu aagaaagaua uccccaucaa gauggugaac     3180

ggaggcaugg aagggauuaa ggagacauac gccuauuaca ucggcgccga cauuuccgcu     3240

gccaauaucc aaggaauugc gcaugauagc cgguauaaau uucgcggccu cgggauccag     3300

ucucuggacg uccuggaaaa gugucagauu gacgugcucg gacacguguc cgucguuaga     3360

agcgagaaac ggaugggcuu cuccuga                                         3387


<210>  3972
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3972
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau ugugcacaaa aaugccagaa ucuuccccgc ugcccaggcg      120

gaaaacaaug ucgagcggcg cacaaacaga caaggccggc gccugacgag acggaagaaa      180

caucgcagag ugcggcucaa ucacauuuuu gaagagucug ggcugaucac cgacuucaca      240

aagguuagca ucaaccuuaa uccuuaccag cugcgcguga aaggacucac cgaugaacug      300

uccaacgagg agcuguuuau ugcucucaag aauaugguca agcauagagg caucucuuau      360

cuggacgaug ccagcgacga uggcaacucc ucuguggggg acuacgccca gauuguuaaa      420

gaaaauagca agcagcuuga gacaaaaacc ccgggacaaa uccagcugga acgguaucag      480

aaguacggcc agcuccgcgg ggauuucaca auugaggagg acggcaaaaa gcacagacug      540

aucaacgugu uucccaccuc cgcuuaucgg gccgaagccc uucgcauucu gcaaacgcag      600

caggaguuca auccucagau caccgaugaa uuuaucaaca gcuaccucca aauucugaca      660

ggaaagagaa aauauuacca ugggcccgga aaugagaagu cucggaccga cuacggcaga      720

uauacaacca aaaaggauuc cgaagacgag uacaucacgc uggacaacau uuucggcauc      780

cucauuggga aaugcacauu uuauccugag gaauaccgcg cugcgaaggc cagcuauacc      840

gcucaggagu ucaaccugcu uaaugaucug aacaaucuca caguccccac cgaaaccaaa      900

aagcugucug aggaacagaa gaaccaaauc aucacauacg ugaaaaauga gaaggccaug      960

ggaccugcua aacuuuuuaa guauauugcc aaacugcucg cgugugacgu ggccgauauc     1020

aagggcuacc ggauugacaa guccgauaaa ccggagaucc acacguucga ggcuuacaga     1080

aagaugaaaa cccuggagac aauugacguc gaacagaugg agcgcgaaaa gcuggauaaa     1140

cucgccuaug uucugacccu uaacacagag cgggagggga uccaggaagc uaucgagcac     1200

gaauuuagcg acggaaccuu cucucaggag caaguggaug agcuggugca guucagaaag     1260

uccaauagcu ccauuuuugg caaggggugg cauaacuucu cugucaaacu caugauggaa     1320

cugauccccg agcuuuacgc cacgagcgaa gaacagauga caauucugac ccgccucgga     1380

aagcagaaac ggaccuccag cucuaauaag acaaaauaua ucgacgaaaa gagacugacc     1440

gaggagauuu acaacccugu gguugccaag uccgugcgcc aagcuaucaa aauugucaau     1500

gcggccauca aggaauaugg cgauuuugac aacaucguga uugagauggc ucgggaaaug     1560

aaugaggacg augagaaaaa ggccaucaaa aagauucagg aagccaacaa aagcgagaag     1620

gacgcugcca ugagaaaggc ggcuaaucag uacaacggca aagccgaacu gccccacucu     1680

guuuuccaug ggcacaagca gcucgccaca aaaauccgcc uguggcacca acagggagag     1740

cggugccuuu auacgggcaa gaccauuagc auccaugauc ugaucaacaa uccuaaccag     1800

uuugaaauug accacauccu cccccugucc auuacauucg augacucucu ggcuaauaaa     1860

gugcuugucu acgccaccgc uaaccaggag aaggggcaaa gaacaccgua ccaggcccuc     1920

gauagcaugg acgaugcgug guccuuucgg gaacugaagg ccuucgugcg cgagagcaaa     1980

acccugucua auaagaaaaa ggaguaucuc cugacggaag aggacaucuc caaauuugau     2040

gucagaaaga aguucauuga acggaaccuu guggacacca gauacgcuag ccgcguugug     2100

cugaaugccc uccaggagca uuuucgggcu agaaaaaucg auacaaaggu cucugugguu     2160

cgcggacaau ucaccuccca gcugagacgg cacuggggca ucgacaaaac acgcgacacc     2220

uaucaucacc acgccgugga ugcgcugauu aucgccgcua gcucccagcu uaaccucugg     2280

aagaaacaga agaauacgcu ggucucuuac agcgaggacc aacugcucga uauugaaaca     2340

ggggagcuga ucuccgacga ugaauauaag gagucugugu uuaaagcccc uuaccagcau     2400

uucguggaca cccuuaagag caaagaguuc gaagauucca uucuguuuag cuaucagguu     2460

gacucuaagu ucaacagaaa aaucuccgau gcuacaaucu acgccacccg gcaggccaag     2520

gucggcaaag acaagaagga ugagaccuac gugcucggaa aaauuaagga caucuauagc     2580

caagcuggcu acgaugcguu uauuaaaauc uaucagaagg acaaaucuaa guuccugaug     2640

uaccgccacg acccccagac auuugaaaag gugauugagc cuauccugga aaauuauccc     2700

aacaaagaga ugaaugaaaa ggggaaagag guccccugua acccuuuccu uaaguacaaa     2760

gaggaacaug gagauuauau uagaaaguac uccaagaaag gcaaugggcc ggagaucaag     2820

agccucaaau acuaugacuc uaagcuggga aaccacaucg auauuacgcc caaaaacagc     2880

aauaacaagg ugguucugca guccgugagc ccuuggcggg ccgacgucua cuuuaauaag     2940

accacaggca aauaugaaau ccucgggcug aaguacgcug aucuucaauu cgagaaaggc     3000

accggaacau auaagauuuc ucaggaaaaa uacaacgaca ucaagaagaa agagggcgug     3060

gauuccgaca gcgaguuuaa guucacccug uauaaaaaug aucuccugcu gauuaaggac     3120

acggaaacaa aagagcagca acuuuuucgc uuccucucca gaucuaugcc caacaagaaa     3180

cacuacguug aacugaagcc uuacagcaag gauaaauuug agaagaauga gacccugauc     3240

gaaauccucg ggcccaccga caaauccgga cggugcauua agggccuggc caaaucuaac     3300

cuuagcaucu ucaaggugcg cacagacauu cuggggaauc agcauaucau uaagaacgag     3360

ggagauaaac cgaagcucga cuucuga                                         3387


<210>  3973
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3973
augaccaaga uccugggccu cgaccugggg acaaacagca uuggaugggc cguggucgau       60

gagaaaaagg agaaaauccu uggcaugggg uccagaauuu uccccgaggg aguugugaau      120

aucggcgaag gggagggacg ggaaacgucu aagaacgcua gccgcaccga cgccagaggc      180

acacggcgcc aguuuuucag acggcgccug agaaaacggu accuccugcg cgagcuugcg      240

aagaagggcc ugugcccuau uaauuauacc cucgugaaag aguggaacga agcucaaauc      300

uuuaagaaua aacagcugca ggagugguuc aagcugaacc cguacgaacu cagauccaaa      360

gccauccagg agaagauuuc ucuggaggaa cuugggcgga ucuuuuauca cuucauucaa      420

cgcagaggau uucagagcaa uucccggucu gccggcgucg auaacaucga aaagagcgug      480

auuuucaaag gggacacaaa gaccggaaaa aucggcauuu ccgaaacacu ggagagcauc      540

aagaauacca aaacgcucgg gucuuaccug cauaccaucu aucccaagca gaacaaaccu      600

uacuccggac acaaggaacg cauuagaaau cgguauacaa ccagacagau guacaucuac      660

gaguuugaga caauuuggga auaucaaaag caguucuaca gcgagcuuuc uaacgaacug      720

aaaaccguuc ucggcggccg caagaaagau ggguauaagg aggacggaau ccuguuucau      780

cagcggcccc ugagauccca gaaacaccuc gugggcuacu guagcuucga accuaagaag      840

acgaaaugcc aaauuaauac aauccccuuu gagcuguucc gcaucuauca gugggucaac      900

accguggauu guaaugagaa gguuacaucu gaagaccggg ggaaaauggu ggagcuucug      960

cucuccaagg aaaaagucag cuucaaggag auuagaaagg ugaucaacaa auccgaugga     1020

uacuaucagu uuaacuacaa gaaugacgau aaaauugugg gcucuuacac caucagcaac     1080

cuguccaaua agaaauucuu ugggaagaag ugguucgacu uuagcgauaa agaacaagag     1140

gacauuuggc auguccuuuc uuucuuugac gaucgcgaca agcugaaaca guaugcuauc     1200

aaguacuggg gauucaacga ggaaaaagcc guuaagaucu ccaaauuuaa ucucaaggag     1260

ggcuauagca accugucucg gaaggcuauu cacaauaucc ugccuuuccu caaacugggc     1320

uuuauguacg auguggccgu ggggcuugga ggcgucaaga acgcgcuggc cgacaaaugg     1380

auugaaaaug agcaguucau ccucgauaac gugccgagcg uugugagauc caauuuuaaa     1440

gggggauaua uugagccccu gaaacagaug cuuaaguacg aguucgacgu cucugauaag     1500

caacugcuca aacuguauca ccauagcucc gcuaucgaaa ccacagaacu gcucaagaaa     1560

cugccuauua acagcgaggc cgacaaggaa auccagucua ucaaaaaucc cgugguuauu     1620

acggcucuuu ucgagauccg caagcuggug aacgagauua ucgauaccua cggcaaguuu     1680

gacgaaauua aagucgagcu cgcccgggau cugaaggcgu ccaaaagcaa gagauacgac     1740

acacgccggg aacagcugag acuugagaaa gaaaaugacc ggaucaagau cgagcucgag     1800

aaccuggggc agcgcauuaa ccacaucacc auucugaagu auaaacucug ggaagagugc     1860

gauaagagau guccuuucac aggcaaaaau aucgaaguga acgagcuguu uucuggagaa     1920

guccaaauug agcauaucuu ccccuggucc cggagccuua augacuccuu uaugaacaag     1980

acccugugcu ucgccgauga gaauagagcu aaaggcaaca agacgccgua cgaauuuuau     2040

ucugagcagg gggaacagaa augggaggag aucaagauuc aggcccucag cuguuucaag     2100

aauaaaccua acuaccccaa ugcuuaucaa aaguuuaaac aguucgugaa gaaaaagcac     2160

gacgaagauu uuaucucccg ccagcugaac gacacacggu acauuucuaa ggaggccaaa     2220

aauuaucuga gcaagaucug caacaauguu cagguggccc cuggacaaau gaccgcuacc     2280

cuuagacaca aauggggccu caacacaauu cugaauaagg augacgauac caaaacacgc     2340

gacgaucaua gacaccaugc gaucgacgcc cuggucaugg cuuguuccac gcggagccac     2400

cuccaggaac ugucuaagcg caacagauac cagaaggauu acgaguucaa agacuuuccc     2460

augcccuggc aauucuuccg gcaggacgcc gaaaaguccg uggagcagau ccuuguuagc     2520

cauaaaaagc ugaacaccau ucucacagug cgcacccaca aaacaaagaa gaaugggguc     2580

guguauaaaa acaucggagu ggccgcuaga ggccagcugc acaaggagac cguuuuuggg     2640

aaacggacgg ccccugaugg cauugaagcg uaccaugucc gcaagccgau cgagucucug     2700

uccacccaca aacauauuaa uaagguggug gacgaaacaa ucagacuucu cauucugaag     2760

caagucaaca aacugggagg cuucgugaag gggaaaaucc ccgagggaac cuuuuauaau     2820

acagaugaaa acggcaauaa gcagccucag aucuuccucc ccaacaaaaa ugggaacccu     2880

guucccauua agaaaauccg gcugcgcgag aauauuggag gcgcugagca gcuuaagagc     2940

accgugaacc aacacgucaa uccgagaucu aaccaccaug ugcugaucua caaggacuuu     3000

gaugacaauc ucaaagaaaa gguugugacg uucuggacag ccgucgagcg gaaaaagcag     3060

aaacaggccg uguuucagcu gccugaaaac gggaaggaga uuguuaccac acugcaaauc     3120

aacgauaugu uccuucucgg ccugaaggac gaugaaauca auugggagca ccccaaauuu     3180

acccugcuca aggagcaucu guauagagug cagaaauuca ccagcggaga cuacuauuuu     3240

cgcaagcaca uugaauccaa ccuuaauggc gagcugggga aggcuuacca guacaucaag     3300

ggauucggcg aagggaagac aggauggaaa acguucaacc cuauuaagau caaagucagc     3360

gugucuggca agauugagaa aguguga                                         3387


<210>  3974
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3974
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgc ugggauccuu       60

aagaaagaua ccggagagau uauccacgcc aauuccagac uguuccccgc ggcuacagcc      120

gacaacaaug uucuccggcg cucuagcaga caggcccggc gcuccacgag acggaagcgc      180

cauagagcug ugcggcugca cgaucuuuuu gaagacuacg gccugcucac cgauuucucu      240

aaagugagca uuaaccugaa uccuuauaag cugcgcgucg aggggaugga cauccaacuc      300

acaaccgaag aacuguuuau cgcccuuaaa aacauuauca agagacgggg aauuuccuac      360

cuggaugacg cuucugaaga uggcgugaca guuagcuccg acuauggcaa ggccguggag      420

gagaaucgca aacuccugag cgaacagacc ccggggcaga uccagcuuga gagauucgaa      480

aaguacggac aaguccgggg cgauuuuaca guggaggaga acggggaaaa acgcagacug      540

auuaauguuu ucucuaccuc cacguaucgg aaggaggcgg aaagaauccu ccgcaaacag      600

caggaguuua acaauaagau uaccgacgau uucaucgacg acuaccugac aauccugacc      660

ggaaagcgga aauauuacca uggccccggg aacaagaaaa gcagaacaga uuacggacgc      720

uauaccacga agaaagacuc ugaaggcaag uacauuacac ucgauaauau cuuuggcgug      780

cugauuggga aaugcaccuu cuauccugac gaguaccggg ccuccaaggc uagcuauaca      840

gcccaggagu uuaaccuucu gaaugaucuc aacaaccuga ccguccccac cgaaacaaag      900

aaacuuucug agaagcaaaa gaagacgauc guggaauacg cuaaaaccgc caagacacug      960

ggagcgucca cccuccugaa guauauugcc aaaauggugg acgcuagcgu cgaucagauc     1020

cacggcuaca gaguugacgu gaauaagaaa ccugagaugc auacauucga uguguaccgc     1080

aagaugcagu cccuggaaac cuuuaacguc aaugagaugc cccgggaggu gcucgacgaa     1140

cuggcccaca uccuuacgcu gaacacagag agagaaggga uugaggaagc uaucaauacc     1200

aaacucaagg gaaccuucaa ccaggaucaa guucuggagc uugugcaguu ccgcaagaau     1260

aacucuagcc uguuuuccaa aggcuggcac aauuucagcc ucaagcugau gauggagcug     1320

auuccugaac ucuaugccac aucugaggaa cagaugacca uccugacacg gcuugggaaa     1380

caaaagucca aagagauuag caagagaacg aaguacaucg acgagaaaga aaucaccgag     1440

gaaauuuaua acccggucgu ggccaagucu guucgccagg cuaucaaaau uaucaaugag     1500

gcgacaaagc aguacggaau uuuugaccau aucgugauug aaauggcccg gcugaccaac     1560

gaggaggaug cuaaaaagga cuauaucaaa agacagaagg ccaaucucga ugaaaagaac     1620

gccgcuaugg agaaagccgc guuccaauac aauggcaaga aagaacugcc cgacagcauc     1680

uuucacggcc auaaggagcu ggcuacaaaa auucggcuuu ggcaccagca gggggaaaag     1740

ugucucuaua ccggaaagaa caucccuauu uccgaucuga uccagaacca auacaaauac     1800

gagauugacc acauccugcc ccucucucug agcuucgaug acucccuuag caauaagguc     1860

cuggugcucg ccacggccaa ccaggagaaa ggccagcgca ccccuuuuca ggcucuggau     1920

agaauggacg augccugguc uuaucgggag uucaaguccu acgucaaaga cagcaagcug     1980

cuugggaaua agaaaaagga uuaucuccug acagaggaag acaucucuaa aauugaggug     2040

aagcaaaaau uuaucgaaag aaaccugguu gacacccgcu acuccagccg ggugguccuc     2100

aaugcucugc aggauuucua uaagaaccau cacuuugaca caaccauuuc cgugguuaga     2160

ggacaguuca cgucucagcu ucgcagaaag uggggccugg agaaaagccg ggagacauac     2220

caucaccaug ccguggaugc gcucaucauu gccgcuuccu cucaacugcg ccuguggaag     2280

aaacagaaua acccccuuau cuuuuauaag gaagggcagu ucgucgacag cgagaccggc     2340

gaaauccucu cccugaauga ugacgaguac aaagagcugg uguucaaggc cccguacgau     2400

cacuuugugg acacacucag aagcaaaacc uucgaagauu cuauucuguu uuccuaucaa     2460

guugacagca aguacaaccg gaagaucucu gaugcuacca uuuaugccac acgcaaagcg     2520

aaacuugaca aagauaaguc caaagagacg uacguccuga gcaagaucaa ggacauuuau     2580

ucucaggccg gauacgacgc uuucaucaaa aucuauaaua aggauaaaag caaguuucuc     2640

auguaccaca aagacuccca gaccuucgaa aaggugauug aggaaauccu gagaacauac     2700

ccuagcaagg aguuuaacga uaaaaauaag gaggugcccu gcaacccuuu cgaaaaauau     2760

cggcaggaga auggccccau ucgcaaguac ucuaaaaagg ggaacggacc cgaaaucaag     2820

ucccugaaau auuacgacaa gaacauuccu gagagcuuua ucaauauuac cccguccaac     2880

ucuaaaaaua aggucauccu uagacaacuc aaccccuggc ggacagaugu guauuucaau     2940

caucagaccg aaaaauacga gcugcugggc cucaaguaug ccgaccugca guuugagaag     3000

aaaacgggga cauacagcau caccauugau aaguacaacg aaaucaaaga gaaggaagga     3060

guuggcgagc aguccgaguu caaauuuacc cuuuauaaga augaccugcu ccugauuaaa     3120

gauacaaaga acaaugaaaa gcaaaucuuc cgcuuccugu cuagaaccau gccuaacgug     3180

aaacacuacg ucgagcuuaa gcccuauaau aaagcuaagu uugacgggaa ccagccucug     3240

auuacaaucu ucggcaaugu ggccaaagga ggccaguguc ucaaggggcu gaacaagccc     3300

aauaucagca uuuacaaaau caagacggau guucucggaa acaaauauuu uauuaagaaa     3360

gaaggcgaca agccgaagcu gaaauucuga                                      3390


<210>  3975
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3975
auggccgaca gaaucagccu gggccucgau auuggggugg cuuccgucgg auucucuguu       60

cuggacaucg auaagggcaa agugauugag cuugggagcc ggcuguuuaa cgccaccgug      120

gcggaaggaa aucaggaccg cagauccaug cggggcgcuc gcagacuccu gaaccggaau      180

aagcaacgca gacaggauac agggaaacuu uuccagaagu uuggacugcu cgacaacuuc      240

aauaaagaua acuuuuacga cacguucaau cagaacugga aucccuauga gcugcggguc      300

aagggccuga ccgaacaccu cacaaagaaa gagcuggccg aaucucuuua ccaaaucgug      360

aagcaucgcg gcauuagcua ugcccugaaa gaugcugacg ccgaugaagg gggaaccgac      420

uacuccgcuu cucucaagau caacagccag gagcuggccg agaaaacccc ugcgcagauc      480

cagcuucaaa gacugaauga uuauggcaag guucggggac agguggucau uggggacgac      540

ccggauaacc agaaggugcu ccugaauguu uuucccacau ccgccuacga gaaagaagcu      600

aagcagauca uugccacaca aaaacaguuc uauccugaga uccugaccga cgaguuuacg      660

aaggaauacu gcaaaauucu cacccgcaag agagauuacu ucgugggccc cgggaacgag      720

aaaagccgga cagacuaugg aaucuacaag accgauggcc gcacacugga caaucuuuuu      780

gaggaacuga uuggccacga uaagaucuau ccugacgaac ucagagcuuc ugccgcgucc      840

uacaccgccc agcuguucaa cguccuuaac gaucugaaua accuccggau ccugagcuau      900

gaggacggga aacugacgaa ggaggauaaa gaaaagauua ucgcugagau uaaaucuaau      960

acaaccacaa ucaacaugcu caaugugauu aagaaggugg ccggauguuc caaagaagac     1020

aucaagggcu uuagagucaa cgagaaagac aagcccgaaa ucagcucuau ggcuguuuac     1080

cgcaaagugc auaaggaucu gcuuaaggug ggggucgaca uuuccgauug gccuguggag     1140

uucaucgaca aacugagcuu cauucucacc cugaauaccg agaacggaga aauccggaag     1200

caacuuaaua acagacuggu uccggaguuu gauuuccuca augacgaucu gauucagcug     1260

aucaucgaca acaaagauuc cuuugaaauu aagacaaaua acaaauggca ccgcuucucu     1320

cucaagacga ugaauaagcu gaugcccgag augcuugaac ggccugugga gcagaugacc     1380

cugcucaacg agaugggccu ggucaaaaag gacaaaaaga gauuugaaaa caauaaauau     1440

cugcccuaca aggagaucgc caaagauauu uucaacccug uggccagcaa guccguucgc     1500

gaagcucuua agaucgugaa ugcgguccuc aaaaaguacg ggcauauuga cuaucuggug     1560

aucgagaugc cccgggauaa aaaccugaag gaggaacagg acaauauuaa agaguuucaa     1620

aacaagaaua agaaagccaa ggacgcugcc uucgaagccu uugucaaaag cgugggaucu     1680

gagcagagag uuaaggaagc ucucuccaaa aaccgcaagc ugcagaugaa gaugcggcuu     1740

ugguaccagc aacaggagau cgauccguau aauggcgaga caaucgacgc caccgaucug     1800

auuaacaauc cugacaaauu cgaaaucgau cacauuaucc cccagagcau uucuuuugac     1860

gauagcauca acaauaagac acucugcuuc gcguccauga accaggugaa aggccaaaag     1920

accccuuacg aguuuaugcu ggaagggcac ggacagggcu augacaaauu caaggcuacg     1980

guccuggaua auaagaacuu cgacagaaac aaacgcaaga auuaccuuuu ugaggaaaac     2040

gugucugaua ucgagacacg gaaaagauuc cucagccgga aucugguuga cacccgcuau     2100

uccagcagag ugguccugaa cucucuccag gacuuuuucc augagaaguc cgccgauacc     2160

aaagugacag ugauucgggg gaaguuuacc agcaauauga gaaaacacug gcauaucgac     2220

aagacacgcg aaacguucca ccaccaugcc auugaugcuu cuaucauugc cgcuaccccc     2280

uuucugcgga uguggaagaa aggaggcaca aucuuccccg uuaaggucgg ggaggaaucc     2340

aucgacauug agaccggcga aauccuugau gacaaaaagu uugauaaagu gauguacgag     2400

gagccuuaca acggauucgu gagcgaaauu augaaugccg acgauagaau caaguuuucc     2460

caccaggucg acaagaaaau gaaccgcaag gugucugaug cgacaauuua uagcaccaga     2520

acgggcaaac uggccaagga caaaaaggau gcugaguaca ucguugccaa ggugaaagac     2580

aucuauuccg ucgacggguu caagaaauuu aagaaagugu acgauaagga caagucuaaa     2640

uuccuccugu auaaguacga uccgcggacc uucagcgaac uggagcgcau uaucuccgac     2700

ugucccgaua aaguugagaa ggugcaaaca aauggaaaag ucaaggcugu ggacguuagc     2760

ccuuuugaaa uguauagacg guaccauggc augauuaaga aauacucuaa gaaagggaac     2820

ggacccgcca ucaagcagcu uaaauaucuc gauaagaaac ugggcuccca cauugacauc     2880

accccugcga augccaacgg gaagcaugug auucugcaga gccucaagcc cuggcgcaca     2940

gaugucuacc ugaaucacga gaccggcgaa uaugagauca ugggaaucaa auacucugac     3000

cuuaaguuca acaaaaaugu gggcuauggg auuaagaaag auaaguaccu ggaaaucaag     3060

aaaaacgagg agguguccga aaagagcgag uuuauguucu cucucuauag aaaagaccgg     3120

guccgcauuc aaaacaugaa gacgggagaa agcguugagc ugcuguuuug guccagaaau     3180

uucagcaaca aaaaguacgc ugaacuuaag ccgaucuuua aauucgugac agaggaugac     3240

gacuggccug uguacggcca cgccaagacc cagauucuca aacggcuggu ccccaaggau     3300

ugcaaaaucu ggaaggugaa uacaaccauc cugggggacc cuuauuaccu cgagaaggaa     3360

ucugauaacc ccaaagacau ucuggauuga                                      3390


<210>  3976
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3976
augaccaacg gcaagauccu ggggcucgac auuggaaucg ccagcguggg cgucgggauu       60

aucgaggcua aaacaggaaa gguugugcac gccaauucca gacuguucuc ugcggcuaac      120

gccgaaaaua acgccgagcg gcgcggcuuu agagggagcc ggcgccuuaa uagacggaaa      180

aagcaucgcg ugaaaagagu ccgggaucug uucgaaaagu acggaauugu gacggacuuu      240

cgcaaccuca aucugaaccc cuaugagcuu agaguuaagg gccugaccga acagcucaca      300

aaugaggagc uguucgcugc ccugcggacc aucuccaaac gcagaggcau cucuuaccuc      360

gaugacgcug aagaugacag cacagggucc accgauuaug ccaagucuau ugacgagaac      420

cggcgccugc uuaaaacaaa gaccccugga caaauccagc uggaaagacu cgagaaauac      480

ggccagcugc gggggaauuu uacgguguau gaugagaacg gagaagcgca cagacuuauu      540

aaugucuuca gcaccuccga cuacgagaag gaagcccgca agauccugga gacacaggcu      600

gauuauaaca aaaagauuac cgccgaauuu aucgacgauu acguggagau ucucacacaa      660

aaacggaagu acuaucaugg cccggggaau gagaaaagca gaaccgacua cggacgcuuc      720

cggacggacg gcacaacccu ggaaaacauc uuuggcaucc ugauugggaa gugcucuuuc      780

uaucccgaug aguacagagc uuccaaagcc agcuauacag cgcaggaaua caacuuucuc      840

aaugaccuga acaaucuuaa gguuccuacc gagaccggaa agcugucuac agaacagaaa      900

gaggcccucg uggaguucgc uaaguccacg gccacccugg gccccgcuaa acuucugaag      960

gaaaucgcca aaauucucga uuguaagguc gacgagauca agggguaucg cgaagaugac     1020

aaaggaaagc cugaucugca cacauuugag cccuaccgga aacugaaguu caaccucgac     1080

agcgugaaua uugaugaccu gucuagagaa gugcuugaua aacuggccga cauccucacc     1140

cugaacacag agcgcgaggg caucgaagac gcuauuaagc ggaaucuucc uaaccaguuc     1200

accgaggaac aaaucuccga gauuaucaau gucagaaaga gccaguccac ggcguuuaac     1260

aaaggguggc auucuuucag cgccaagcug augaaugagc ucauuccgga acuguacgcu     1320

acauccgaug agcagaugac cauccugacc cgccucgaaa aauuuaaggu uaaaaagaaa     1380

agcucuaaaa acaucaagac aauugacgag aaagaaauca ccgaugagau uuauaauccc     1440

gugguggcca aguccguccg gcagacaauc aaaauuauca acgccgcugu gaagaaauac     1500

ggagacuucg auaagauugu uaucgagaug ccuagagaca agaaugccga ugacgaaaag     1560

aaguuuaucg auaaacggaa caaggagaac aaaaaggaaa aggacgaugc gcugaaacgc     1620

gcugccuauc uuuacaaugg cacggacaag cugcccgaug agguguucca cgaaaacaaa     1680

caacucgaga ccaagauuag acugugguau cagcagggcg agcggugccu guacagcggg     1740

aaaccuaucc ccauucaaga acuuguccac aauucuaaca auuuugagau cgaccauauu     1800

cucccgcuga gccuguccuu cgacgauucu cucgccaaca aggugcuggu uuauagcugg     1860

acaaaucagg aaaagggaca gagaaccccu uaccagguga ucgacuccau ggaugcugcc     1920

uggagcuuuc gcgagaugaa agacuacguc cuuaagcaaa aaggccuggg gaagaaaaag     1980

cgggauuauc uccugacaac cgaaaacauc gacaagauug aggugaaaaa gaaauucauc     2040

gagagaaauc uggucgauac gcgcuacgcu ucuagagugg uucuuaacuc ccuccagagc     2100

gcccugcggg aacugggaaa ggacaccaaa gugucugucg ugcgcggcca guuuacaucc     2160

cagcucagac ggaaguggaa aauugauaag agccgcgaga ccuaucacca ucacgcgguu     2220

gacgcccuga ucauugcugc cuccucucaa cuuaagcugu gggaaaaaca ggauaauccc     2280

auguucgugg acuacgggaa caaucagguc guggauaagc agacaggcga gauccucagc     2340

guguccgacg acgaguauaa agaacugguu uuucaaccuc ccuaccaggg auucgucaac     2400

accaucucua gcaagggcuu cgaggaugaa auucuguuuu ccuaucaggu ggacagcaaa     2460

uacaauagaa aggugucuga ugcuacgauc uacuccacac ggaaggccaa aauugggaag     2520

gacaaaaagg aggaaaccua uguccuugga aaaaucaagg auauuuacag ccagaacggc     2580

uucgacacau uugugaagaa auauaacaag gauaaaaccc aauuccucau guaccagaag     2640

gacccccuga ccugggagaa uguuaucgag gugauccugc gcgauuaucc uacaacgaaa     2700

aagucugaag acgggaagaa cgaugucaaa uguaauccgu uugaggaaua cagacgggag     2760

aacggacuca uuugcaagua uuccaaaaag ggcaaaggga cccccaucaa gagccugaag     2820

uacuacgaca aaaagcuggg caauuguauu gacaucacac cucaggaauc uaaaaaccgc     2880

gugguucuua gacaaauuag ccccuggcgg gcggauaucu auuucaaucc ugagacccuc     2940

aaguacgagc ugaugggacu gaaauauucc gaccuuagcu uugaaaaggg cacagggaaa     3000

uaccacauuu cucaggagaa guaugaugcc aucaaggaaa aagagggagu gggcaaggac     3060

uccgaguuca aauuuacccu cuacagaaac gaucugaucc ugauuaagga cacgcucaau     3120

aacugcgaac gcaugcugcg guucgggagc aaaaaugaua cauccaagca uuaugucgag     3180

cuuaagcccc uggaaaaagg aaccuuugac ucugaggaag agauccuccc ggugcugggc     3240

aagguugcua aaagcgggca guucauuaag ggccugaaca aaccuaauau cuccauuuac     3300

aaggugagaa ccgauguccu uggaaacaag uacuuuguga aaaaggaggg cgacaaaccc     3360

aagcuggauu ucaaaaauaa caagaaguga                                      3390


<210>  3977
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3977
augagcaacg gcaagauccu ggggcucgac auuggagugg ccuccgucgg cguugggauc       60

auugauucua aaaccggaaa ugugauccac gcuaacagca gacuguucuc cgccgcgaau      120

gcugagaaca augccgaacg gcgcggcuuu agaggggccc ggcgccuuac aagacggaag      180

aaacaucgcg ugaagagagu ccgggaccug uucgagaaau acgauauuuc uacggacuuu      240

cgcaaccuca aucugaaccc cuaugaacuu agagugaagg gacugaccga gcagcucaca      300

aaugaggaac uguucgcugc ccugcggacc aucgcuaagc gcagaggcau cagcuaccuc      360

gaugacgccg aggaugacuc cacaggcucu agcgauuaug cgaaauccau ugacgaaaac      420

cggcgccugc uuaagaccaa aacaccuggg caaauccagc uggagagacu cgaaaaguac      480

ggacagcugc ggggcaauuu uaccguuuau gaugagaacg gggaggccca cagacuuauu      540

aauguguuca gcacgucuga cuacaaaaac gaagcucgca agauccugga gacccagucc      600

aauuauaaca agcaaauuac agaugaauuu aucgaggacu acauugaaau ccucacccag      660

aaacggaagu acuaucaugg accgggcaac gagaaaagca gaacagacua cgggcgcuuc      720

cggaccgaug gaacgacacu ggagaauauc uuuggcauuc ugaucggcaa gugcucuuuc      780

uaucccgaag aguacagagc cuccaaagcu agcuauaccg cccaggaauu uaacuuccuc      840

aaugaccuga acaaucuuaa ggucccuaca gagaccggga aacugucuac cgaacagaag      900

gaguaccucg uggauuuugc gaagaaaucc aaggcccugg gagcuagcaa acuucugaag      960

gagauugcca aaaucguuga cuguuccgug gaugacauua agggcuaucg cgucgauaac     1020

aaggacaaac ccgaucucca cacauucgaa ccuuaccgga agcugaaauu caaucugucu     1080

agcaucgaca ucgaugagcu cuccagagaa acgcuggaca aacuugcuga cauucugacc     1140

cucaacacag agcgcgaagg gaucgaggau gccauuaagc ggaaucugcc cagccaauuu     1200

accgaggaac agaucucuga gauugugcag aucagaaaga accaguccag cgccuucaau     1260

aagggauggc auucuuuuag cgcuaaacuu augaacgaac ugaucccuga gcucuacgug     1320

acauccgagg aacaaaugac cauucugacg cgccuggaga aguucaaagu caauaagaaa     1380

ucuagcaaga acacaaagac caucgacgaa aaagagauua ccgaugaaau cuauaauccg     1440

guuguggcga aguccgugcg gcagacaauu aaaaucauua acgccgcugu caagaaauac     1500

ggcgacuuug auaagaucgu gaucgagaug cccagagaca aaaacgccga ggaugaaaag     1560

aaguucauug acaaaaagca gaaagagaau aagaaagaaa aggaugacag ccucaagcgg     1620

gccgcuuuuc uguauaacgg gaccgauaau cuuccugacg gaguuuucca cggcaacaaa     1680

gagcugaaga caaaaauccg ccucugguac caacagggcg aaagaugccu guauucuggg     1740

aagcugauuu ccauccacga ucuugugcau aauagcaaca aauuugagau ugaccacauc     1800

cucccccugu cucuguccuu cgacgauagc cucgccaaua agguccuggu guacgcgugg     1860

acgaaccagg agaagggaca gaaaaccccu uaucaaguua ucgacuccau ggauacagcu     1920

uggucuuuuc gggaaaugaa ggacuacgug cuuaaacaga agggccuggg gaaaaagaag     1980

ugugaguacc uccugaccac agaaaauauu gauaaaaucg aggucaagaa aaaguucauu     2040

gaaagaaacc ugguggacac ccgcuaugcc agccgggucg ugcuuaauuc ccuccagacg     2100

gcccugaaag agcugggaaa ggauaccaaa guuucugugg ucagaggcca guuuacaagc     2160

caacuccgca gaaaguggaa caucgacaag ucccgggaga ccuaccauca ccacgcugug     2220

gaugcccuga uuaucgcugc cagcucucag cuuaaacugu ggcagaagca ggaaaauccc     2280

auguucgagu ccuaugggga aaaccaaguu gugaauaaag agacaggcga gauccucagc     2340

auuucugacg auaaguacaa agaacugguc uuccagccgc cuuaucaggg auuugugaac     2400

accaucucca gcaagggcuu cgaggacgaa auucuguuuu cuuaccaggu ggauagcaag     2460

uucaaccgca aaguuuccga cgcgacgauc uauagcacaa gaaaggccaa acuugggaag     2520

gacaaaaagg augagaccua cguccucgga aagauuaaag acaucuacuc ucaagauggc     2580

uuugacacau ucaucaagcg guauaaaaag gauaaaaccc aguuucugau guaccagaag     2640

gacccccuga ccugggaaaa ugugauugag gugauccucc gcgauuaucc uuccgagaag     2700

cugagcgaag acgggaaaaa gacagucaaa ugcaaccccu ucgaggaaua cagacgggag     2760

aauggacuua uuuguaagua uuccaaaaag ggcaacggga cgcccaucaa gucucugaaa     2820

uacuaugaua agaaacucgg caauugcauu gacaucaccc cugaaaagag caaaaaccgc     2880

gugguucuga gacaaauuuc cccguggcgg gcugauaucu acuuuaaucu ggagacacuu     2940

aaguacgagc ucaugggacu gaaauauucu gaccugagcu ucgaaaaggg caccgggaag     3000

uaccauaucu cccaggagaa auaugacgcc auucgcgaaa aggagggaau cggcaaaaag     3060

ucugaauuua aauucacacu cuacagaaac gaucugauuc uuaucaagga cacccugaau     3120

aacugugagc ggaugcucag auuugggagc aagaaugaua cguccaaaca cuauguggag     3180

cugaagcccc uggaaaaagg aacauucgac agcgaggaag agauucuucc uguccugggc     3240

aagguggcua aaucugggca guucaucaag ggccucaaca agcccaauau cuccauuuac     3300

aaaguucgca ccgaugugcu gggaaacaag uuuuucauca aaaaggaggg cgacaaaccu     3360

aagcucgauu uuaagaauaa caacaaauga                                      3390


<210>  3978
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3978
augagcaacg gcaagauccu ggggcucgac auuggagugg ccuccgucgg cguugggauc       60

auugaugcua aaaccggaaa ugugauccac gccaacucua gacuguucag cgcggcuaau      120

gccgagaaca augccgaacg gcgcggcuuu agaggggcuc ggcgccuuac aagacggaag      180

aaacaucgcg ugaagagagu ccgggaccug uucgagaaau acgauauuuc cacggacuuu      240

cgcaaccuca aucugaaccc cuaugaacuu agagugaagg gacugaccga gcagcucaca      300

aaugaggaac uguucgccgc ucugcggacc aucgccaagc gcagaggcau cucuuaccuc      360

gaugacgcgg aggaugacag cacaggcucc ucugauuaug ccaaaagcau ugacgaaaac      420

cggcgccugc uuaagaccaa aacaccuggg caaauccagc uggagagacu cgaaaaguac      480

ggacagcugc ggggcaauuu uaccguuuau gaugagaacg gggaggcuca cagacuuauu      540

aauguguucu ccacgagcga cuacaaaaac gaagcccgca agauccugga gacccagucu      600

aauuauaaca agcaaauuac agaugaauuu aucgaggacu acauugaaau ccucacccag      660

aaacggaagu acuaucaugg accgggcaac gagaaaucca gaacagacua cgggcgcuuc      720

cggaccgaug gaacgacacu ggagaauauc uuuggcauuc ugaucggcaa gugcagcuuc      780

uaucccgaag aguacagagc uucuaaagcc uccuauaccg cgcaggaauu uaacuuccuc      840

aaugaccuga acaaucuuaa ggucccuaca gagaccggga aacugagcac cgaacagaag      900

gaguaccucg uggauuuugc caagaaaucu aaggcucugg gagccuccaa acuucugaag      960

gagauugcua aaaucguuga cuguagcgug gaugacauua agggcuaucg cgucgauaac     1020

aaggacaaac ccgaucucca cacauucgaa ccuuaccgga agcugaaauu caaucugucc     1080

ucuaucgaca ucgaugagcu cagcagagaa acgcuggaca aacuugccga cauucugacc     1140

cucaacacag agcgcgaagg gaucgaggau accauuaagc ggaaucugcc cucccaauuu     1200

acagaggaac agaucagcga gauugugcag aucagaaaga accagucuuc cgccuucaau     1260

aagggauggc auagcuuuuc ugcuaaacuu augaacgaac ugaucccuga gcucuacgug     1320

accagcgagg aacaaaugac gauucugaca cgccuggaga aguucaaagu caauaagaaa     1380

uccucuaaga acaccaagac caucgacgaa aaagagauua cagaugaaau cuauaauccg     1440

guuguggcca agagcgugcg gcagaccauu aaaaucauua acgcggcugu caagaaauac     1500

ggcgacuuug auaagaucgu gaucgagaug cccagagaca aaaacgccga ggaugaaaag     1560

aaguucauug acaaaaagga gaaagaaaau aagaaagaga aggaugacuc ccucaagcgg     1620

gccgcuuuuc uguauaacgg gacagauaau cuuccugacg gaguuuucca cggcaacaaa     1680

gaacugaaga cgaaaauccg ccucugguac cagcaaggcg agagaugccu guauagcggg     1740

aagcugauuu cuauccacga ucuugugcau aauuccaaca aauuugagau ugaccacauc     1800

cucccccuga gccugucuuu cgacgauucc cucgccaaua agguccuggu guacgcgugg     1860

accaaccagg aaaagggaca gaaaacaccu uaucagguua ucgacagcau ggaugcugcc     1920

ugguccuuuc gggagaugaa ggacuacgug cuuaaacaaa agggcauugg gaaaaagaag     1980

agagaauacc ugcucaccac agagaauauc gauaaaauug aagucaagaa aaaguucauc     2040

gagcgcaacc ugguggacac ccgguaugcc ucuagagucg ugcugaauag ccuucagacg     2100

gcucucaaag agcugggaaa ggauaccaaa guuuccgugg uccgcggcca guuuacaucu     2160

cagcugagac ggaaguggaa gauugacaaa agccgcgaaa ccuaccauca ccacgccgug     2220

gaugcucuca ucaucgccgc guccgggcaa cugaaacuuu ggcagaagca ugagaaccug     2280

auguucgaaa auuauggcga gaaccagguu gugaauaagg agacaggaga aauucucagc     2340

aucucugacg augaguacaa agaacugguc uuccagcccc cguaucaagg cuuugugaac     2400

accauuucca gcaagacguu cgaggacgaa auccuguuuu cuuaccaggu ggauuccaag     2460

uucaacagaa aaguuagcga cgccacaauu uauucuaccc ggaaggcuaa acuugggaag     2520

gacaaaaagg aggagacaua cguccucgga aagaucaaag auaucuacac ccaggacggc     2580

uuugauaccu ucauuaagcg cuauaaaaag gacaaaacac aguuucugau guaccaaaag     2640

gauccucuga cgugggaaaa ugugaucgag gugauucuca gagacuaucc cagcgaaaag     2700

cuguccgagg augggaaaaa gaccgucaaa uguaacccuu ucgaagagua ccggcgcgag     2760

aauggacuua ucugcaagua uagcaaaaag ggcaacggga cacccauuaa gucucugaaa     2820

uacuaugaca agaaacucgg caauuguauc gauauuaccc ccgaaaaguc caaaaacaga     2880

gugguucugc ggcagaucag cccuuggcgc gccgacaucu acuuuaaucu ggagacacuu     2940

aaguacgaac ucaugggacu gaaauauucc gaucugucuu ucgagaaggg caccgggaag     3000

uaccacauua gccaggaaaa auaugacgcu aucagagaga aggagggaau uggcaaaaag     3060

uccgaauuua aauucacgcu cuaccggaac gaccugaucc uuauuaagga uacacugaau     3120

aacugcgaga gaaugcuccg cuuugggucu aagaaugaca ccagcaaaca uuauguggaa     3180

cugaagccgc uggagaaagg aaccuucgau uccgaggaag agauccuucc cguccugggc     3240

aagguggcca aaucugggca auucaucaag ggccucaaca agccuaauau uagcaucuac     3300

aaaguucgga cagacgugcu gggaaacaag uuuuucauua aaaaggaagg cgauaaaccc     3360

aagcucgacu uuaagaauaa caacaaauga                                      3390


<210>  3979
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3979
augaacaaga uccugggccu cgaccugggg accaauagca uuggaugggc ccuuaucgag       60

aacaauauua uccugggcau gggguccaga guguuccccg aaggagucgu uaaccucggc      120

gagggggaag gaagagagac aucuaaaaau gcuagccgca cggaagccag aggcgugcgg      180

cgccagauuu uuagacggcg ccugagaaag cgguaccuuc ugaaagagcu cgcgaagcac      240

aaccugugcc cuaucucccc gacccugauc aaagauugga auggcacaga cauuuucaag      300

aacgagcuca ucauucaaug guuucagcug aaucccuaug aacuucgcgc uaaggccauc      360

caggaggaaa uuacccugau cgagcucggg agaauuuucu accauaugau ccagcggcgc      420

ggauuucugu cuaacagcag auccgccggc gcugauguga aagagacauc uaccaucuuc      480

aagggggacg ccaaaauugg aaagaucggc auuagcgaua cacuuaaauc caucgaacaa      540

aauaagaccc uggggagcuu ucuccacucu auucagccua aggagaacga acccuucucc      600

ggagagcugg aacggauccg caauagauau acgacccggc agauguacau cgacgaguuu      660

gagacaauuu gggaguucca gaaacaauau cauagcgagc ugaacucuaa ucucaagacc      720

auccugggcg gccgcaaaaa ggauaacuac aaagaagacg ggguccuuuu ucaccagaga      780

ccucugcggu cccagaagca ucucguggga uucuguagcu uugagcccaa aaagacaaag      840

ugcaaacugu cugcuauucc uuucgaacuu uucagaaucu aucagugggu uaacacccug      900

uccuacgaug ugaauggcgu ccgccaaaag auuacgcagg aggagcggaa aaagaucauc      960

gaccuccugc ugagcaaaga aaagccgucc uuuaaggaga uuagaaaagc caucgggaag     1020

cucgauggau cuuaccaguu caacuauaaa gacgaugaca agauuguggg cagccacaca     1080

aucuccaauc ugagcaacaa aaaguuuuuc gggaauaagu gguuugacuu cucugaaaaa     1140

gagcaagaaa acauuuggca cauccuuucc uuuuucgaug accgcgagaa gcugaaagag     1200

uacgcgauua agaauuggag cuuugaugaa gaacgggccg uuaaaaucuc uaaguucaac     1260

cucaaggacg gauauagcaa ucuguccaga aaagcuaucc uuaacauucu gcccuuucuc     1320

cagaagggcu ucauguacga uguggccguc ggccuggcug gggugaaaaa uacccugucu     1380

gugaacuggg aagaacauga gcaguuuguc cucgacaaug uuccugaaau cgugcgcagc     1440

aaccugaagg gaggcuauau ugagccccuu aaaaauaugc ugaagaacga guucaacugu     1500

aaugauaaac agcucgagaa gcuguaccac cauuccagcg ccaucgaaac agagaacauu     1560

cuggaccggc uuccugugaa ucuccacgcg gauagagaga uccaaaagau caaaaacccc     1620

gucgugauua ccgcccuguu cgaaauccgc aagcugguua augaggugau ugacaccuau     1680

gggaaaccga acgaaaucaa ggucgagcuc gcucgggauc ugaaagccuc uaaguccaau     1740

agacuugaag cucggaagcg ccagaaagac cuggagaagc agaacgacag agugaaacuc     1800

gagcuggaaa aucugaacca gcggagcaau cacauuaaca uccuuaagua caaacucugg     1860

gaggaaugca aucauacaug uccuuuuacg ggacaaauca uuaccaucuc ucagcuguuc     1920

acaggcgagg uucagauuga acacaucuuu cccuggucca gaagccugaa cgauuccuuc     1980

aauaacaaga cccucugcuu ugccgacgag aaccgcgcca aggggaauaa aacaccuuac     2040

gagcuguauu cugaacaggg cgaucaaaag ugggaggcua uuaaacuuca ggcgcugagc     2100

uguuucaaga ccaaagccaa cuaccccaau ugcuauaaca aguuuaagca uuucgugaaa     2160

aagaaacacg acgaagauuu uaucucccgg cagcucaaug acacgagaua caucucuaag     2220

gaggcuaaaa acuaucugag caagauuugu uccaaugucc ugguggcccc cggacagaug     2280

acagccaccc uucgccauca cuggggccuc aacagcaucc ugaaucugga ggaugacacc     2340

aaaacaagag augaccaccg gcaucacgcu auugaugccc ucgucauggc gugcucuacc     2400

cgcucccauc ugcaagaacu uagcaagaga aaccgguaca auaagaacua ugaccugaaa     2460

gauuucccug acccguggca gaauuuucgc caggacgcug agaacgccgu ggcccaaauc     2520

cucguuucuc acaagaaauc caagagccug cugacaguga gaacgcacaa aaccauuaag     2580

aagggggucg aacauaaaaa uaucggagug gcugcccggg gccagcuuca caaggagucu     2640

guuuacgggc uccgcaaagc ucccaacagc aacaaggccu uccauauuag aaaaccucug     2700

gaaucccuga caaccgagaa gcagcucgag aagaucgugg augacagcau ccggcugcuu     2760

auucugaaac gcaucaauau uaucggaggc uuuauuaagg ggaaaauccc caaggaaaca     2820

uucuucgucg uggaugagaa cggcaucaaa cagccucaaa uuuuucuccc caauagaaac     2880

ggaaauccgg ugccuaucaa gaagguucgg auuagagaaa acaucggcgg ggcggagaaa     2940

cugaaggaaa auaccaacca guacgucaau ccccgcucua accaccaugu gauuguguau     3000

gaggacucca auggaggccu gaaagaugac gucgugacgu ucuggaccgc cguugagcgg     3060

aagagacagg ggcaggaugu guuuaaacuu ccuaacgacg gagaaaagau cgucagcaca     3120

cugcaaauca augauauguu ccuccugggc cucaacgacg augacauuau cacccugaca     3180

uccaaggagc uuaaaaagca ccuguuuaaa gugcagaccc ucucuagcuc cuucuacgaa     3240

uuucgccugu cuacggauag cacacagaau aagaacacca auuccacagu uuuccggcgg     3300

auucagucuu uugggauggg caaaaccgga uggaaggcuu ucaaccccau caaagugaag     3360

cugaacagca uuggcgaggu caagaucauu uga                                  3393


<210>  3980
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3980
augaccaacg gcaagauccu ggggcucgac auuggaaucg ccagcguggg cgucgggauu       60

aucgaggcua aaacaggaaa gguugugcac gccaauucca gacuguucuc ugcggcuaac      120

gccgaaaaua acgccgagcg gcgcggcuuu agagggagcc ggcgccuuaa uagacggaaa      180

aagcaucgcg ugaaaagagu ccgggaucug uucgaaaagu acggaauugu gacggacuuu      240

cgcaaccuca aucugaaccc cuaugagcuu agaguuaagg gccugaccga acagcucaca      300

aaugaggagc uguucgcugc ccugcggacc aucuccaaac gcagaggcau cucuuaccuc      360

gaugacgcug aagaugacag cacagggucc accgauuaug ccaagucuau ugacgagaac      420

cggcgccugc uuaaaacaaa gaccccugga caaauccagc uggaaagacu cgagaaauac      480

ggccagcugc gggggaauuu uacgguguau gaugagaacg gagaagcgca cagacuuauu      540

aaugucuuca gcaccuccga cuacgagaag gaagcccgca agauccugga gacacaggcu      600

gauuauaaca aaaagauuac cgccgaauuu aucgacgauu acguggagau ucucacacaa      660

aaacggaagu acuaucaugg cccggggaau gagaaaagca gaaccgacua cggacgcuuc      720

cggacggacg gcacaacccu ggaaaacauc uuuggcaucc ugauugggaa gugcucuuuc      780

uaucccgaug aguacagagc uuccaaagcc agcuauacag cgcaggaaua caacuuucuc      840

aaugaccuga acaaucuuaa gguuccuacc gagaccggaa agcugucuac agaacagaaa      900

gaggcccucg uggaguucgc uaaguccacg gccacccugg gccccgcuaa acuucugaag      960

gaaaucgcca aaauucucga uuguaagguc gacgagauca agggguaucg cgaagaugac     1020

aaaggaaagc cugaucugca cacauuugag cccuaccgga aacugaaguu caaccucgac     1080

agcgugaaua uugaugaccu gucuagagaa gugcuugaua aacuggccga cauccucacc     1140

cugaacacag agcgcgaggg caucgaagac gcuauuaagc ggaaucuucc uaaccaguuc     1200

accgaggaac aaaucuccga gauuaucaau gucagaaaga gccaguccac ggcguuuaac     1260

aaaggguggc auucuuucag cgccaagcug augaaugagc ucauuccgga acuguacgcu     1320

acauccgaug agcagaugac cauccugacc cgccucgaaa aauuuaaggu uaaaaagaaa     1380

agcucuaaaa acaucaagac aauugacgag aaagaaauca ccgaugagau uuauaauccc     1440

gugguggcca aguccguccg gcagacaauc aaaauuauca acgccgcugu gaagaaauac     1500

ggagacuucg auaagauugu uaucgagaug ccuagagaca agaaugccga ugacgaaaag     1560

aaguuuaucg auaaacggaa caaggagaac aaaaaggaaa aggacgaugc gcugaaacgc     1620

gcugccuauc uuuacaaugg cacggacaag cugcccgaug agguguucca cggcaacaaa     1680

caacucgaaa ccaagauuag acugugguau cagcaggggg agcggugccu guacagcgga     1740

aaaccuaucc ccauucaaga gcuuguccac aauucuaaca auuuugaaau cgaccauauu     1800

cucccgcuga gccuguccuu cgacgauucu cucgccaaca aggugcuggu uuauagcugg     1860

acaaaucagg agaagggcca gagaaccccu uaccagguga ucgacuccau ggaugcugcc     1920

uggagcuuuc gcgaaaugaa agacuacguc cuuaagcaaa aagggcuggg aaagaaaaag     1980

cgggauuauc uccugacaac cgagaacauc gacaagauug aagugaaaaa gaaauucauc     2040

gagagaaauc uggucgauac gcgcuacgcu ucuagagugg uucuuaacuc ccuccagagc     2100

gcccugcggg agcugggcaa ggacaccaaa gugucugucg ugcgcgggca guuuacaucc     2160

cagcucagac ggaaguggaa aauugauaag agccgcgaaa ccuaucacca ucacgcgguu     2220

gacgcccuga ucauugcugc cuccucucaa cuuaagcugu gggagaaaca ggauaauccc     2280

auguucgugg acuacggcaa caaucagguc guggauaagc agacaggaga aauccucagc     2340

guguccgaca acgaguauaa agagcugguu uuucaaccuc ccuaccaggg cuucgucaau     2400

accaucucua gcaagggguu cgaagacgag auucuguuuu ccuaucaggu ggauagcaaa     2460

uacaacagaa aggugucuga cgcuacgauc uacuccacac ggaaggccaa aauuggaaag     2520

gauaaaaagg aagagaccua uguccuuggc aaaaucaagg acauuuacag ccagaacggg     2580

uucgauacau uugugaagaa auauaauaag gacaaaaccc aauuccucau guaccagaag     2640

gauccccuga ccugggaaaa cguuaucgag gugauccugg gagacuaucc uacaacgaaa     2700

aagucugagg auggcaagaa ugacgucaaa uguaacccgu uugaagagua ccgcagagaa     2760

aaugggcuca uuugcaagua uuccaaaaag ggcaaaggaa cccccaucaa gagccugaag     2820

uacuacgaua aaaagcuggg caacuguauu gacaucacac cugaggaauc ucggaauaaa     2880

gugauucuuc agagcaucaa ccccuggcgc gcggacguuu auuucaaucc ugagacccuc     2940

aaguacgagc ugauggggcu gaaauauucc gaucuuagcu uugaaaaggg aacaggcaac     3000

uaccacauuu cucaagagaa auaugacgcc aucaaggaaa aggaggggau cggcaaaaag     3060

uccgaguuca aauuuacccu cuacagaaau gaucugauuc ugaucaagga cauugcuagc     3120

ggagaacagg agaucuaucg guuccucucc agaacgaugc ccaacgugaa ucauuacguc     3180

gaacugaaac cguacgauaa ggagaaguuu gacaacgugc aggaacuugu ugaggcccug     3240

ggggaggcug auaaaguggg ccgcugcauu aagggacuca auaaaccuaa caucucuauc     3300

uauaaggucc ggacagacgu gcugggcaac aaauacuucg ugaagaagga aggggauaaa     3360

cccaagcugg acuuuaaaaa uaacaagaaa uga                                  3393


<210>  3981
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3981
augaccaacg gcaagauccu ggggcucgac auuggaaucg ccagcguggg cgucgggauu       60

aucgaggcua aaacaggaaa gguugugcac gccaauucca gacuguucuc ugcggcuaac      120

gccgaaaaua acgccgagcg gcgcggcuuu agagggagcc ggcgccuuaa uagacggaaa      180

aagcaucgcg ugaaaagagu ccgggaucug uucgaaaagu acgagauugu gacggacuuu      240

cgcaaccuca aucugaaccc cuaugaacuu agaguuaagg gacugaccga gcagcucaca      300

aaugaggaac uguucgcugc ccugcggacc aucuccaaac gcagaggcau cucuuaccuc      360

gaugacgcug aggaugacag cacaggcucc accgauuaug ccaagucuau ugacgaaaac      420

cggcgccugc uuaaaacaaa gaccccuggg caaauccagc uggagagacu cgagaaauac      480

ggacagcugc ggggcaauuu uacgguguau gaugaaaacg gggaggcgca cagacuuauu      540

aaugucuuca gcaccuccga cuacgaaaag gaggcccgca agauccugga aacacaggcu      600

gauuauaaca aaaagauuac cgccgaguuu aucgacgauu acguggagau ucucacacaa      660

aaacggaagu acuaucaugg accgggcaau gagaaaagca gaaccgacua cgggcgcuuc      720

cggacggacg gaacaacccu ggaaaacauc uuuggcaucc ugauuggcaa gugcucuuuc      780

uaucccgaug aauacagagc uuccaaagcc agcuauacag cgcaggagua caacuuucuc      840

aaugaccuga acaaucuuaa gguuccuacc gaaaccggga agcugucuac agagcagaaa      900

gaggcccucg uggaguucgc uaaguccacg gccacccugg gacccgcuaa acuucugaag      960

gaaaucgcca aaauucucga uuguaagguc gacgaaauca agggcuaucg cgaggaugac     1020

aaagggaagc cugaucugca cacauuugaa cccuaccgga aacugaaguu caaccucgac     1080

agcgugaaua uugaugaccu gucuagagag gugcuugaua aacuggccga cauccucacc     1140

cugaacacag agcgcgaagg aaucgaggac gcuauucggc auaaucuucc uaaccaguuc     1200

accgaaggcc aaaucuccga gauuaucaag gucagaaaga gccaguccac ggcguuuaau     1260

aaaggguggc acucuuucag cgccaagcug augaacgagc ucauuccgga acuguacgcu     1320

acauccgaug agcagaugac cauccugacc cgccucgaaa aauuuaaggu uaauaaaaag     1380

agcucuaaga acacaaaaac caucgacgag aaggaaguga cagaugagau uuauaauccc     1440

guggucgcca aauccgugcg gcagacgauc aagauuauca acgccgcugu uaaaaaguac     1500

ggagacuucg auaaaauugu gaucgagaug ccuagagaca agaaugccga ugacgaaaag     1560

aaauuuauug auaagcggaa caaagagaac aagaaagaaa aggacgaugc gcugaagcgc     1620

gcugccuauc uuuacaaugg caccgacaaa cugcccgaug aggucuucca cggcaacaag     1680

caacucgaaa caaaaaucag acugugguau cagcaggggg agcggugccu guacagcgga     1740

aagccuaucc ccauucaaga gcuugugcau aauucuaaca auuuugaaau cgaccacauu     1800

cucccgcuga gccuguccuu cgacgauucu cucgccaaca aaguucuggu guaugcuugg     1860

accaaucagg agaagggcca gaagacaccu uaccagguca ucgacagcau ggaugccgcu     1920

ugguccuuua gagaaaugaa agacuacgug cuuaagcaaa aagggcuggg aaagaaaaag     1980

cgcgauuauc uccugaccac ggagaacauu gacaagaucg aagucaaaaa gaaauucauc     2040

gagcggaauc ugguggauac cagauacgcc agccgcguug ugcuuaacuc ucuccagucc     2100

gcgcugagag agcugggcaa ggacacaaaa auuagcguca uccgggggca guuuaccucu     2160

cagcuccgca gaaaguggaa aauugauaag ucccgggaaa cauaucauca ccacgccgug     2220

gacgcucuga ucauugccgc uagcucccaa cuuaagcugu gggagaaaca ggauaauccc     2280

auguucguug acuacggcaa caaucaggug gucgauaagc agaccggaga aauccucucu     2340

gugagcgacg acgaguauaa agagcuggug uuucaaccuc ccuaccaggg cuucguuaac     2400

augaucuccu cuaagggguu cgaagaugag auucuguuua gcuaucaggu cgacuccaaa     2460

uacaaucgca aggugagcga ugccacgauc uacucuacaa gaaaggcgaa aauuggaaag     2520

gacaaaaagg aagagaccua ugugcuuggc aaaaucaagg auauuuacuc ccagaacggg     2580

uucgacacau uuaucaagaa auauaacaag gauaaaaccc aauuccucau guaccagaag     2640

gacccccuga ccugggaaaa ugucaucgag gugauucugc gggauuaucc uacaacgaaa     2700

aagagcgagg acggaaagaa cgauguuaaa uguaauccgu uugaagagua ccgcagagaa     2760

aacggccucg ugugcaagua uucuaaaaag gggaaaggca cccccaucaa gucccugaag     2820

uacuacgaca aaaagcuggg aaauuguauu gacaucacac cugagggcag caaaaacgaa     2880

gucgugcuuc agucucucaa ucccuggcgg gccgauguuu auuucaaccc ugagacccug     2940

aaguacgagc ugcuugggcu caaauauagc gaccuguccu uugaaaaggg aacaggcaaa     3000

uaccauauua gccaagagaa guaugaugug aucaaggaaa aagaggggau uggaaagaaa     3060

ucugaguuca aguuuacccu guaccgcaau gaccucaucc ugaucaaaga uacggcuucc     3120

ggcgaacagg agauuuauag auuccuuagc cggacaaugc ccaacgucaa gcacuacgcc     3180

gaacugaagc cguacgacaa agagaaguuu gauaaugugc aggaacucgu ugaggcucug     3240

ggggaggccg acaaaguggg cagaugcauc aagggacuga acaaauccaa ucuuucuauu     3300

uauaaggucc gcaccgaugu gcugggcaac aaguacuucg ugaaaaagga aggggacaaa     3360

ccuaagcucg auuuuaaaaa uaacaagaag uga                                  3393


<210>  3982
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3982
augaccaacg gcaagauccu ggggcucgac auuggaaucg ccagcguggg cgucgggauu       60

aucgaggcua aaacaggaaa gguugugcac gccaauucca gacuguucuc ugcggcuaac      120

gccgaaaaua acgccgagcg gcgcggcuuu agagggagcc ggcgccuuaa uagacggaaa      180

aagcaucgcg ugaaaagagu ccgggaucug uucgaaaagu acggaauugu gacggacuuu      240

cgcaaccuca aucugaaccc cuaugagcuu agaguuaagg gccugaccga acagcucaca      300

aaugaggagc uguucgcugc ccugcggacc aucuccaaac gcagaggcau cucuuaccuc      360

gaugacgcug aagaugacag cacagggucc accgauuaug ccaagucuau ugacgagaac      420

cggcgccugc uuaaaacaaa gaccccugga caaauccagc uggaaagacu cgagaaauac      480

ggccagcugc gggggaauuu uacgguguau gaugagaacg gagaagcgca cagacuuauu      540

aaugucuuca gcaccuccga cuacgagaag gaagcccgca agauccugga gacacaggcu      600

gauuauaaca aaaagauuac cgccgaauuu aucgacgauu acguggagau ucucacacaa      660

aaacggaagu acuaucaugg cccggggaau gagaaaagca gaaccgacua cggacgcuuc      720

cggacggacg gcacaacccu ggaaaacauc uuuggcaucc ugauugggaa gugcaacuuc      780

uaucccgaug aguacagagc uucuaaagcc uccuauacag cgcaggaaua caauuuucuc      840

aacgaccuga auaaccuuaa gguuccuacc gagaccggaa agcugagcac agaacagaaa      900

gagucucucg uggaguucgc caagaauacg gcuauccugg gccccgccaa acuucugaag      960

gaaauugcua aaauccucga uuguaagguc gacgagauua agggguaucg cgaagaugac     1020

aaaggaaagc cugaucugca caccuuugag cccuaccgga aacugaaguu caaccucgac     1080

uccgugaaua ucgauuaccu gagcagagaa gugcuugaca aacuggccga uauccucaca     1140

cugaacaccg agcgcgaggg cauugaagac gccaucaagc ggaaucuucc uaaccaguuc     1200

acagaggaac aaauuucuga gaucauuaau gucagaaagu cccagagcac cgcuuuuaac     1260

aaaggguggc auuccuucuc ugcgaagcug augaaugagc ucaucccgga acuguaugcc     1320

acgagcgacg agcagaugac aauccugacc cgccucgaaa aauuuaaggu uaaaaagaag     1380

uccagcaaaa acauuaagac caucgaugag aaagaaauua cagacgagau cuacaauccc     1440

gugguggcua agucuguccg gcagaccauu aaaaucauua acgccgccgu gaagaaauau     1500

ggagauuucg acaagaucgu uaucgagaug ccuagagaua agaacgcuga cgaugaaaag     1560

aaguuuauua auaaacggaa caaggagaau aaaaaggaaa aggacgaugc ccugaaacgc     1620

gcggcuuacc uuuauaacgg cacagacaag cugcccgaug agguguucca cggcaauaaa     1680

caacucgaaa cgaagaucag acugugguac cagcaggggg agcggugccu guauuccgga     1740

aaaccuauuc ccauccaaga gcuuguccac aacagcaaua acuuugaaau ugaccauauc     1800

cucccgcugu cucugagcuu cgaugacucc cucgccaaua aggugcuggu uuacucuugg     1860

accaaccagg agaagggcca gaaaacaccu uaccagguga ucgacagcau ggaugccgcu     1920

ugguccuuua gagaaaugaa ggaguauguc cuuaaacaaa aggggcuggg caaaaagaag     1980

cgcgacuacc uccugaccac agaaaauauu gauaaaaucg aggugaagaa aaaguucauu     2040

gagcggaacc uggucgacac cagauaugcc agccgcgugg uucuuaauuc ucuccagucc     2100

gcucugagag aacugggaaa agauacgaag gugagcgucg ugcgggggca guuuaccucu     2160

cagcuccgca gaaaauggaa gaucgacaag ucccgggaga cauaccacca ucacgccguu     2220

gaugcgcuga uuaucgccgc uagcucccaa cuuaaacugu gggaaaagca ggacaacccc     2280

auguucgugg auuauggcaa uaaccagguc guggacaaac agaccggaga gauccucucu     2340

gugagcgaua acgaguacaa ggaacugguu uuucaaccuc ccuaucaggg cuucgucaau     2400

acaauuuccu cuaaaggguu cgaggacgaa auccuguuua gcuaccaggu ggauuccaag     2460

uacaaccgca aggugagcga cgccaccauu uauucuacga gaaaagcuaa gaucggaaaa     2520

gacaagaaag aggaaacaua cguccuuggc aagauuaagg auaucuauuc ccagaauggg     2580

uucgacaccu uugugaaaaa guacaacaaa gauaagacac aauuccucau guaucagaaa     2640

gacccccuga ccugggagaa uguuaucgag gugauucugg gagauuaccc uaccacaaag     2700

aaaagcgaag acggcaaaaa cgaugucaag uguaauccgu uugaggaaua ucggcgcgag     2760

aacgggcuca ucugcaaaua cucuaagaaa ggcaagggaa cgcccauuaa gucccugaaa     2820

uacuaugaca agaaacuugg caauuguauc gauauuaccc cugaagagag cagaaacaag     2880

gugauccugc agucuauuaa ucccuggcgg gccgacguuu acuucaaccc ugagacacuc     2940

aaauaugaac ugauggggcu gaaguacagc gaucuuuccu uugagaaagg aaccggcaau     3000

uaucacauca gccaagaaaa guacgacgcg aucaaggaga aagaagggau uggaaagaaa     3060

ucugaguuca aguuuacacu cuaucgcaac gaccugaucc ugauuaaaga uaucgccucc     3120

ggcgagcagg aaauuuacag auuccucagc cggaccaugc ccaaugugaa ccauuacguc     3180

gagcugaagc cguaugacaa ggaaaaauuu gauaacgugc aggagcuugu ugaggcucug     3240

ggggaagccg acaagguggg cagaugcauc aaaucccuca auaagccuaa caucucuauu     3300

uacaaagucc gcacggaugu gcugggaaau aaguauuucg ugaagaaaga gggcgacaag     3360

cccaaacugg auuuuaagaa caauaaaaag uga                                  3393


<210>  3983
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3983
augagcaacg gcaagauccu ggggcucgac auuggagugg ccuccgucgg cguugggauc       60

auugaugcua aaaccggaaa ggugauccac gccucuagca gacuguucuc cgcggcuaau      120

gccgagaaca augccgaacg gcgcggcuuu agaggggcuc ggcgccuuac aagacggaaa      180

aagcaucgca uuaaaagagu gcgggaccug uucgagaagu acgaaaucgu cacggauuuu      240

ucuaaccuca aucugaaccc cuaugagcuu cgcgugaagg gacugaccga acagcucaca      300

aaugaggagc uguucgccgc ucugagaacc aucgccaaac ggcgcggcau uagcuaccuc      360

gacgaugcgg aagacgauuc cacaggcucu agcgacuaug ccaaguccau cgaggaaaac      420

agacggcugc uuaaaaauau gaccccuggg caaauucagc uggagcgccu cgagaaguac      480

ggacagcuga gaggcaacuu uacaguuuau gaugaaaaug gggaggcuca ccggcuuauc      540

aacguguuca gcaccucuga cuacaaaaau gaagccagaa agauucugga gacgcagucc      600

aacuauaaua agcaaaucac cgaugaauuu auugaggacu acaucgagau ccucacacag      660

aaacgcaagu acuaucaugg accgggcaac gaaaagagcc ggaccgauua cgggagauau      720

cgcacagacg gaaccacgcu ggagaacauu uucggcaucc ugauuggcaa gugcacauuu      780

uaccccgacg aauaucggac cucuaaggcu uccuacacag cccaggagua uaauuuccuc      840

aacgaucuga auaaccuuaa ggucccuacc gaaaccggga aacugagcac agagcagaag      900

cacuaccucg uggacuuugc gaagucuucc aaagcccugg gaguugcuaa acuucugaaa      960

gagaucgcca agauugugga auguagcguc gaugacauca aaggcuacag agaggauaau     1020

aaggggaagc ccgaccucca uacguucgaa ccuuaucgca aacugaaguu uaaccugucc     1080

cucaucgaua uugacgagcu gucucgggaa acccuugaua aacuggcuga cauccucaca     1140

cugaauaccg agagagaggg aauugaagau gccaucaagc gcaaccuucc caaucaauuc     1200

acagaggaac agauugugga gcuggugaac guccggaaaa aucagagcuc cgccuucaac     1260

aagggcuggc acagcuuuuc uguuaagcuc augaaugagc ugaucccuga acuguacgcu     1320

uccagcgagg aacagaugac cauccucacg agacuggaga aauucaaggu gaacaaaaag     1380

ucuagcgaaa auacaaaaac cauuaacgag aaggagauca ccgacgaaau uuauaacccg     1440

guggucgcga aguccguguc ucaaacaguu aaaaucauua augccgcugu gaagaaauac     1500

ggggacuuug auaagaucgu cauugagaug ccccgcgaca aaaacgccga ugacgaaaag     1560

aaauucaucg auaagaagca gaaagagaau gccaaggaag uggaggacgc ucuuaaacgg     1620

gccgcguauc uguacaacgg aaccgauaag cucccugacg gcguuuuuca cggcaauaaa     1680

cagcugaaga caaagaucag acugugguau caacaggggg agcggugccu uuacagcgga     1740

aaacccauuu ccauccagga ucucgugcau aacagcaaua acuucgaaau ugaccacauc     1800

cugccucugu cucucuccuu ugaugacagc cuggcuaaua agguccuugu guacgccugg     1860

acgaaccagg agaaaggcca aaagaccccc uaucagguca uugauucuau ggacgccgcu     1920

ugguccuucc gcgaaaugaa agacuacgug cugaaucaga aggggaucgg aaagaaaaag     1980

agagaguauc uccugacaac cgaaaacauc gauaaaauug agguuaagaa aaaguuuauc     2040

gagcggaauc ugguggacac aagauacgcc agccgcgucg ugcuuaacuc cauucaggcu     2100

gcccucaagg aacuggauaa agacaccaag guuucuguga uccggggcca auucacgagc     2160

cagcugagac gcaaauggaa gauugauaaa uccagagaga ccuaucauca ccacgcgguc     2220

gacgcccuca ucaucgcugc cucuagccag cugaaacuuu ggcagaaaca agaaaauccg     2280

auguuugugg auuacgggga gaaccaggug guuaacaagg agacaggcga aauucugucc     2340

gucagcgacg augacuauaa ggagcucgug uuccagccuc ccuaccaggg auuugugaau     2400

accaucucuu ccaaggcuuu cgaagaugag auucuguuca gcuaccaagu cgacucuaag     2460

uauaaccgga aaguguccga ugccacaauc uacagcaccc gcaaggcgaa acugggcaag     2520

gacaagaaag aagagacgua uguucuuggg aagauuaaag acaucuacuc ucaggaugga     2580

uuugacgccu ucaucaagag auauaaaaag gauaagacac aguuucucau guaccaaaaa     2640

gacccuguga ccugggagaa ugucauugaa gugauccugc gggauuaucc cagcgagaag     2700

cuguccgaag acggcaaaaa gaacguuaaa uguaaucccu ucgaggaaua ccgcagagag     2760

aacgggcuca uuugcaagua cagcaagaaa ggaaagggca caccuaucaa aucucugaag     2820

uauuacgaua aaaagcuggg gaauuguauu gacaucaccc cgaagggcuc caaaaacgag     2880

gugguccuuc agagccucaa ucccuggcgg gcugaucugu auuuuaaccc ugacacccug     2940

aaguacgaac uuaugggacu caaauauucc gaucugucuu ucgagaaggg cacagggaaa     3000

uaccauauua gccaggaacg cuaugacgag aucaagguga aagaaggaau cggcaaaaau     3060

uccgaguuua aguucacgcu guacagaaac gaccucauuc ugaucaaaga uaccgagucu     3120

ggggaagagg aacuuuaccg guuucugagc cgcacaaugc ccaauguuaa gcacuaugug     3180

gagcucaaac cuuacgacaa ggagagauau gauggaggcc aggaacugau gaaagucuuc     3240

gggaacgugg ccaauggcgg acaaugccug aagucccuua acaagucuaa ccugagcauu     3300

uacaaagugc ggaccgacgu ccucggcaau aaguuuuuca ucaaaaagga gggggauaaa     3360

cccaagcugg acuuuaagaa caauaaaaag uga                                  3393


<210>  3984
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3984
augaccaacg gcaagauccu ggggcucgac auuggaaucg ccagcguggg cgucgggauu       60

aucgaggcua aaacaggaaa gguugugcac gccaauucca gacuguucuc ugcggcuaac      120

gccgaaaaua acgccgagcg gcgcggcuuu agagggagcc ggcgccuuaa uagacggaaa      180

aagcaucgcg ugaaaagagu ccgggaucug uucgaaaagu acggaauugu gacggacuuu      240

cgcaaccuca aucugaaccc cuaugagcuu agaguuaagg gccugaccga acagcucaaa      300

aaugaggagc uguucgcugc ccugcggaca aucuccaagc gcagaggcau cucuuaccuc      360

gaugacgcug aagaugacag caccgggucc acagauuaug ccaaaucuau ugacgagaac      420

cggcgccugc uuaagaauaa aaccccugga caaauccagc uggaaagacu cgagaaguac      480

ggccagcugc gggggaacuu uacaguguau gaugagaaug gagaagcgca cagacuuauu      540

aacgucuuca gcaccuccga cuacgagaag gaagcccgca aaauccugga gacgcaggcu      600

gauuauaaua agaaaauuac cgccgaauuu aucgacgauu acguggagau ucucacacaa      660

aagcggaaau acuaucaugg cccggggaac gaaaagagca gaaccgacua cggacgcuuc      720

cggacagacg gcaccacgcu ggaaaacauc uuuggcaucc ugauugggaa augcaauuuc      780

uaucccgaug aguacagagc uucuaaggcc uccuauacag cgcaggaaua caacuuucuc      840

aaugaccuga acaaucuuaa gguuagcacc gagacaggaa aacugucuac cgaacagaag      900

gagucccucg uggaguucgc caaaaacacc gcuacacugg gcccugccaa gcugcugaaa      960

gaaaucgcua agauucucga uuguaagguc gacgagauca aaggguaucg cgaagaugac     1020

aagggaaaac ccgaucugca cacguuugag ccuuaccgga agcugaaauu caaucucgaa     1080

agcauuaaca ucgacgaucu gucuagagag gugaucgaca aacuugccga uauucugacc     1140

cucaauacag agcgcgaagg caucgaggac gccauuaagc ggaaccugcc caaucaguuc     1200

accgaggagc aaaucuccga gauuaucaag gugagaaaga gccaguccac agcuuuuaac     1260

aaaggguggc auucuuucag cgcgaaacuu augaaugaac ugaucccuga gcucuacgcc     1320

accuccgacg aacagaugac gauucugaca cgccuggaga aguuuaaggu caacaagaaa     1380

agcucuaaga auaccaaaac caucgaugaa aaggagguua cagacgagau uuauaacccg     1440

gugguggcua aauccguccg gcagaccauc aagauuauca acgccgccgu gaaaaaguac     1500

ggagauuucg acaagauugu uaucgaaaug cccagagaua aaaaugcuga cgaugagaag     1560

aaauuuaucg acaagcggaa caaagaaaau aagaaggaga aagaugacgc ccucaagcgc     1620

gcggcuuauc uguacaacag cucugauaaa cuuccugacg aaguguucca cggcaauaag     1680

caacuggaga caaaaauuag acucugguau cagcagggcg agcggugccu guacagcggg     1740

aagcccaucu ccauucaaga acugguccac aacucuaaua acuuugagau cgaucauauu     1800

cuuccucuca gccuguccuu cgacgacagc cuggccaaua aggugcucgu uuaugccugg     1860

acgaaccagg aaaaaggaca gaagaccccc uaccagguga ucgauucuau ggacgcugcc     1920

ugguccuuua gagagaugaa agauuacguc cugaagcaaa aaggccuugg gaagaagaaa     1980

cgcgacuauc ugcucacaac cgaaaauauc gauaagauug aggugaaaaa gaaauucauc     2040

gagcggaacc uggucgacac aagauacgcu agccgcgugg uucuuaauuc ucuucagucc     2100

gcccucagag aacugggaaa ggauaccaaa gugagcgucg ugcggggcca guuuacgucc     2160

cagcugcgca gaaaguggaa gauugacaaa ucucgggaga ccuaucacca ucacgcgguu     2220

gaugcccuca ucauugcugc cagcucccaa cugaaacuuu gggaaaagca ggacaacccg     2280

auguucgugg auuacgggaa gaaucagguc guggacaaac agacaggcga gauccugucu     2340

gugagcgaug acgaguauaa ggaacucguu uuucaaccuc ccuaccaggg auucgucaac     2400

accaucucca gcaagggcuu cgaggacgaa auucuguuuu cuuaucaggu ggauuccaaa     2460

uacaaccgca aggugagcga cgcuacaauc uacucuacca gaaaagccaa gauugggaaa     2520

gauaagaagg aggaaacgua uguccuggga aaaaucaagg acauuuacuc ccagaauggc     2580

uucgauacau uuaucaaaaa guauaacaaa gacaagaccc aauuccuuau guaccagaag     2640

gauagccuca caugggagaa ugugaucgag guuauucugc gggacuaucc uaccaccaaa     2700

aagucugaag augggaaaaa cgacgugaag uguaaucccu uugaggaaua ccgcagagag     2760

aacggacuga ucugcaaaua uagcaagaag ggcaaaggga cacccauuaa gucccucaaa     2820

uacuacgaua agaaacuggg caauuguauc gacauuacgc cugaagagag ccggaacaag     2880

gucauccuuc agucuauuaa uccguggcgc gcggacgugu auuucaaccc cgagacccug     2940

aaauacgaac ucaugggacu gaaguauucc gaucugagcu uugagaaggg cacagggaau     3000

uaccacaucu cccaagaaaa auaugacgcc aucaaggaga aagaaggaau uggcaagaaa     3060

ucugaguuca aguuuacccu uuacagaaac gaucucaucc ugauuaagga caucgcuagc     3120

ggggagcagg aaauuuaucg guuccugucc agaacaaugc cuaauguuaa ccauuacgug     3180

gagcucaaac ccuacgauaa ggaaaaauuu gacaaugucc aggagcuggu ggaggcccuu     3240

ggagaagcug auaagguugg ccgcugcauc aaagggcuga acaagccuaa caucucuauu     3300

uauaaggugc ggaccgacgu ccucggcaau aaauacuucg ugaagaaaaa gggagauaaa     3360

cccaagcugg acuuuaagaa caauaaaaag uga                                  3393


<210>  3985
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3985
augaccaacg gcaagauccu ggggcucgac auuggaaucg ccagcguggg cgucgggauu       60

aucgaggcua aaacaggaaa gguugugcac gccaauucca gacuguucuc ugcggcuaac      120

gccgaaaaua acgccgagcg gcgcggcuuu agagggagcc ggcgccuuaa uagacggaaa      180

aagcaucgcg ugaaaagagu ccgggaucug uucgaaaagu acggaauugu gacggacuuu      240

cgcaaccuca aucugaaccc cuaugagcuu agaguuaagg gccugaccga acagcucaca      300

aaugaggagc uguucgcugc ccugcggacc aucuccaaac gcagaggcau cucuuaccuc      360

gaugacgcug aagaugacag cacagggucc accgauuaug ccaagucuau ugacgagaac      420

cggcgccugc uuaaaacaaa gaccccugga caaauccagc uggaaagacu cgagaaauac      480

ggccagcugc gggggaauuu uacgguguau gaugagaacg gagaagcgca cagacuuauu      540

aaugucuuca gcaccuccga cuacgagaag gaagcccgca agauccugga gacacaggcu      600

gauuauaaca aaaagauuac cgccgaauuu aucgacgauu acguggagau ucucacacaa      660

aaacggaagu acuaucaugg cccggggaau gagaaaagca gaaccgacua cggacgcuuc      720

cggacggacg gcacaacccu ggaaaacauc uuuggcaucc ugauugggaa gugcucuuuc      780

uaucccgaug aguacagagc uuccaaagcc agcuauacag cgcaggaaua caacuuucuc      840

aaugaccuga acaaucuuaa gguuccuacc gagaccggaa agcugucuac agaacagaaa      900

gaggcccucg uggaguucgc uaaguccacg gccacccugg gccccgcuaa acuucugaag      960

gaaaucgcca aaauucucga uuguaagguc gacgagauca agggguaucg cgaagaugac     1020

aaaggaaagc cugaucugca cacauuugag cccuaccgga aacugaaguu caaccucgac     1080

agcgugaaua uugauuaccu gucuagagaa gugcuugaca aacuggccgg cauccucacc     1140

cugaacacag agcgcgaggg gaucgaagau gcuauuaagc ggaaucuucc uaaccaguuc     1200

accgaggaac aaaucuccga gauuaucaau gucagaaaga gccaguccac ggcguuuaac     1260

aaaggauggc auucuuucag cgccaagcug augaaugagc ucauuccgga acuguaugcu     1320

acauccgacg agcagaugac cauccugacc cgccucgaaa aauuuaaggu uaaaaagaaa     1380

agcucuaaaa acaucaagac aauugacgag aaagaaauca ccgaugagau uuacaauccc     1440

gugguggcca aguccguccg gcagacaauc aaaauuauca acgccgcugu gaagaaauau     1500

ggcgacuucg auaagauugu uaucgagaug ccuagagaca agaaugccga ugacgaaaag     1560

aaguuuauca acaaacggaa caaggagaau aaaaaggaaa aggaugacgc gcugaaacgc     1620

gcugccuacc uuuauaacgg cacggauaag cugcccgacg agguguucca cgggaauaaa     1680

caacucgaaa ccaagauuag acugugguac cagcagggag agcggugccu guauagcggc     1740

aaaccuaucc ccauucaaga gcuuguccac aacucuaaua acuuugaaau cgaucauauu     1800

cucccgcuga gccuguccuu cgacgauucu cucgccaaua aggugcuggu uuacagcugg     1860

acaaaccagg agaaggggca gaaaaccccu uaccagguga ucgacuccau ggacgcugcc     1920

uggagcuuua gagaaaugaa ggaguauguc cuuaaacaaa agggacuggg caaaaagaag     1980

cgcgauuacc uccugacaac cgaaaauauc gacaaaauug aggugaagaa aaaguucauc     2040

gagcggaacc uggucgauac gagauaugcu ucucgcgugg uucuuaauuc ccuccagagc     2100

gcccugagag aacuggggaa agacaccaag gugucugucg ugcggggcca guuuacaucc     2160

cagcuccgca gaaaauggaa gauugauaag agccgggaga ccuaccacca ucacgcgguu     2220

gacgcccuga ucauugcugc cuccucucaa cuuaaacugu gggaaaagca ggauaacccc     2280

auguucgugg acuauggaaa uaaccagguc guggauaaac agacaggcga gauccucagc     2340

guguccgaca augaguacaa ggaacugguu uuucaaccuc ccuaucaggg guucgucaac     2400

accaucucua gcaaaggauu cgaggaugaa auucuguuuu ccuaccaggu ggacagcaag     2460

uacaaccgca aggugucuga ugcuacgauc uauuccacaa gaaaagccaa gauuggcaaa     2520

gacaagaaag aggaaaccua cguccuuggg aagaucaagg acauuuauag ccagaaugga     2580

uucgauacau uugugaaaaa guacaacaaa gacaagaccc aauuccucau guaucagaaa     2640

gauccccuga ccugggagaa uguuaucgag gugauccugg gcgacuaccc uacaacgaag     2700

aagucugaag augggaaaaa cgacgucaag uguaauccgu uugaggaaua ucggcgcgag     2760

aacggccuca uuugcaaaua cuccaagaaa ggaaagggca cccccaucaa gagccugaaa     2820

uacuaugaua agaaacuugg gaauuguauu gacaucacac cugaagaguc uagaaacaag     2880

gugauucugc agagcaucaa ucccuggcgg gcggauguuu acuucaaccc ugagacccuc     2940

aaauaugaac ugaugggacu gaaguacucc gaccuuagcu uugagaaagg cacagggaau     3000

uaucacauuu cucaagaaaa guacgaugcc aucaaggaga aagaaggaau cggcaagaaa     3060

uccgaguuca aguuuacccu cuaucgcaac gaccugauuc ugaucaaaga cauugcuagc     3120

ggggagcagg aaaucuacag auuccucucc cggacgaugc ccaaugugaa ccauuacguc     3180

gagcugaagc cguaugauaa ggaaaaauuu gacaaugugc aggagcuugu ugaggcccug     3240

ggcgaagcug auaagguggg aagaugcauu aaaggccuca acaagccuaa caucucuauc     3300

uacaaagucc gcacagacgu gcuggggaau aaguauuucg ugaagaaaga gggagauaag     3360

cccaaacugg acuuuaagaa caauaaaaag uga                                  3393


<210>  3986
<211>  3396
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3986
auggcccagc acguguucgg ccuggacauc gggauugcua gcgucggaug ggccauccuc       60

ggcgagcaaa gaauuaucga ucuggggguu cggugcuuug acaaggcgga aaccgcuaaa      120

gagggagauc cccuuaaccu gacacgcaga caggcccggc uccugcgcag acggcuuuac      180

cgcagagccu ggcggcugac gcagcucucc cgccugcuga agagaaaagg ccucauugcu      240

gacgccaagc uguucgcuaa agccccuucu uauggggaua gcgcguggga acuucggcgc      300

cagggacugg acagacuccu gaccccgcuu gagugggccc gggugaucua ccaucaaugu      360

aagcaccgcg gcuuucauug gacauccaag gcugaagagg ccaaagcuga uucugacgcc      420

gagggcggga gagugaagca gggacuggcg cacaccaaag cccucaugca ggcuaagaau      480

uaucggagcg ccgcugaaau gguccuggcc gaguuccccg augcccagag aaacaaacgc      540

ggccaauacg acaaggcucu gucccgggug cuccuggggg aagaacuugc gcugcucuuu      600

gccacacaga gacgccuggg aaauccucau gcuucugauu ucuuugagaa gcugauccug      660

ggcgacgggg aucggaaaag cggacucuuc uggcagcaga agcccgcccu guccggcgcc      720

gaccugcuca aaaugcuggg caagugcacc uuugaaaaag gggaguauag agcuccuaag      780

gccagcuucu cuguugaacg ccacgugugg cuuacacggc ugaacaaucu cagaauuguc      840

guggacggac gcucccggcc ccugaacgag gcggaaagac aagcugcccu ucugcucccu      900

uaccagaccg agacgagcaa auauaagacc cugaagaaug ccuuuaucaa agcuggccug      960

uggggagaug ggguucgcuu cggcgggcuc gccuacccgu cucaggcuca aauugacgcc     1020

gagaagacaa aagaucccga agaccaguuu cuggugaaac uuccugcgug gcacgagcug     1080

cggaaggccu ucaaggcugc cggacaugaa gcucucuggc agcagaucuc cacccccgcc     1140

cuggauggcg acccuacacu gcuugaucaa auugcgaccg uccucagcgu guacaaggac     1200

ggcgccgagg ugguccagca gcugagacag cuggcucucc ccgaaccggc cgcuucuauc     1260

gccguucugg agaaaauuuc cuucgauaag uuuagcuccc uuucucugaa agcccuccgg     1320

cgcaucgugc cucugaugca aagcgggcug agauaugacg aggcuguggc gcagaucccc     1380

gaauacggac accauuccca gcggauugag ccuggcgccg cuaagcaccu uuaucucccu     1440

cccuucuacg aagcccagag aaaauaugcc gggaagggag aucacaucgg cagcaugcaa     1500

uuucgcgacg acgcugauau uccccggaac ccggucgugc ugagagcccu gaaucaggcg     1560

cgcaagguug ugaacgcucu caucagagag uacgggucuc ccauugccgu caauaucgaa     1620

auggcccggg accugucccg cccucuugau gagagaaaca aagugaagcg ggcucaggag     1680

gaguuccgcg acagaaauga ucgggcccgc agcgaguuug aaagagacuu cggcuauaaa     1740

cccaaggcgg cugccuuuga gaaauggaug cuguaccggg agcagcucgg acaaugugcc     1800

uacucucagc agccucugga uauccaacgc guucuggacg aucauaacua ugcucaggug     1860

gaccacgccc uucccuacag cagauccuau gaugacucua agaauaacaa gguccucgug     1920

cugacgcaug aaaaccagaa uaaaggcaac cggacagcuu ucgaguaccu gaccagcuuu     1980

ccggaugggg aagacggaga gagauggcgc acauucgucg ccugggugca gggcaauaag     2040

gcguaucgga uggccaaaag aaaccgccuc cugcggaaga auuacggggu ugacgaaucc     2100

aaaggauuua uugauagaaa ccuuaaugac acccgcuaua ucugcaaguu cuuuaaaaac     2160

uacguggagg agcaccuuca acucgcugcc cgggcugaug gcgacaccgc cagacgcugu     2220

gucgugguua augggcagcu gacagccuuc cugcgggcua gauggggccu uacgaaggug     2280

cggggagaua gcgaccgcca ucacgcgcuc gaugccgcug ucguggccgc cugcacccac     2340

ggcaugguga aggcucuggc cgacuacucu agacggaaag aaauuuccuu ccugcaggag     2400

ggguuuccug aucccgaaac aggagagauc cucaacccug cggcuuucga ccgcgccaga     2460

cagcauuuuc ccgaaccuug gacccacuuc gcccaugagc ugaaggcucg gcuuuuuaca     2520

gaugaccugg ccgcgcucag agaggauaug caacgccugg gcagcuauac cacggaagac     2580

cuggggcggc uuagaacacu guucguuucu cgcgcucccc agcggagauc cggaggcgcc     2640

guccacaaag agaccauuua cgcccagccg gaaagccuca agcagcaagg gggcgugauc     2700

gagaaaaucc ugcucaccuc ccugaaacuu caggacuuug auaagcugcu caauccugaa     2760

ucuaacgacc acuucgugga gccccaucgc aaugagcggc uguaugcugc cauuagacag     2820

cgccuggaac aauuuggagg cagagcugau aaggccuucg ggcccgacaa ccuuuuucac     2880

aagccugaua aaaauaacca gccgacagga cccgucgugc ggagcaucaa gcugguucgc     2940

ggcaaacaga ccgggauucc uaucagagga ggccucgcga agaacgacuc caugcugcgg     3000

guggauauuu ucacaaaggc cgggaaauuu caucucgucc ccguguacgu ucaccaucgc     3060

gugacgggcc ugccuaauag agcuaucguc gccuucaagg acgaggauga auggacccuu     3120

auugacgagu cuuucgcuuu ucuguucagc guguauccca acgauuacgu uaaagugaca     3180

cucaagaaag agcagcaauc cggauauuac agcggcgccg accggucuac cggggccaug     3240

aaucuguggg cucacgacag agcggccucc gucggaaagg auggccugau ccgcgggauc     3300

ggagugaaga cagcucuuag cguggaaaaa uuuaacgucg acguucuggg ccggauuuac     3360

cucgccccuc ccgagaccag aucuggccug gccuga                               3396


<210>  3987
<211>  3399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3987
auggcccagu acguguucgg ccuggacauc gggauugcua gcgucggaug ggccguucuc       60

gaugcgcaaa gaaucgugga ccugggcgug cggugcuuug auaaggcuga gaccgccaaa      120

gaaggggacc cccuuaaccu gacacgcaga caggcccggc uccugcgcag acggcuuuau      180

cgcagagcuu ggcggcugac gcagcucgcc cgccuguuca agagagaggg acugauugau      240

gacgcuaaac ucuuugccaa ggcgccuucc uggaccgaaa augccuggga gcugcggcuu      300

cgcggccugg aucaccuccu gacaccgcuu gaaugggcua gagucaucua ccaucugugu      360

aaacaccggg gguuccauug gaccucuaag gccgaggagg cuaaggccga aagcgacgcg      420

gagggaggcc gcgugaaaca gggccucgcc cacacaaagg cucugaugca aguuaaaaac      480

uauagaucca ccgccgaaau ggugcuggcu gaguuucccg gggcccagcg gaauaagaga      540

ggacaguacg auaaagcccu cucucgcguc cugcuuggcg aggaacuggc ucuccuguuc      600

gcgacacagc ggagacuugg gaacccucau gccagcgagc acuuugaaca acugauucuc      660

ggacacggcg accgcaaguc cgggcuguuc uggcagcaga agcccgcucu gucuggagcc      720

gaucuccuga aaaugcuugg caagugcacc uuugagaaaa cggaauaucg ggccccuaag      780

gcuagcuucu ccgccgagag acaugugugg cugacccgcc ucaauaaccu gcggaucguu      840

guggacggca gagcgcgccc ccuuaaugau gcugagcggc aggccgcccu gcuccugccu      900

uaccaaacag aaaccuuuaa auauaagaca cugaaagcug ccuucgucaa gguggggcuc      960

uggggagacg gcgugagauu ugggggacug gcuuacccga gccaggccca ggcggaggcc     1020

gaaaaggcua aagaccccga ggaucaauuc cuugucaagc ugccugccug gcacgaacuc     1080

cgcaaggcuu ucaaagccgc gggccaugag gcccuguggc agcagaucuc uaugcccgcu     1140

cuggaggaag acccuacccu ucucgaucag auugccacgg uucuguccgu guacaaagac     1200

gggacagagg ugguccaaca gcugcggcag cucgcucugc ccgaaccgga ugccagcauc     1260

gccgugcuug agaagauuag auuugacaag uucucuuccc ugagccucaa agcucugcgg     1320

cgcaucguuc cucugaugca gggaggccuu agauaugaug aagcgguggc ccaaauuccc     1380

gaguacggcc accacucuca gcggaucgac ccuggggcua ccagacaucu cuaucugccu     1440

cccuuuuacg aggcccagcg caaguaugcc ggaaaaggcg aucggauugg guccaugcag     1500

uucagagaag acgcugaugu cccccgcaac ccggugguuc ugagagcccu caaucaagcg     1560

cggaaggugg ucaacgcucu gauccgcgag uacggaagcc ccguggccgu caauaucgaa     1620

auggccagag accuuucccg gccucuggac gagcgcaaca aagugaagag agcucaggag     1680

gaauuucggg aucgcaauga caaggccaga ucugaguucg aacgggauuu uggcuauaaa     1740

cccaaggggg cgguuuucga gaaauggaug cucuaccgcg aacagcuggg ccagugugcu     1800

uacagccaac agccucugga cauucagaga gugcuugaug accccaacua ugcccaaguc     1860

gaucacgccc ucccguacuc ccggagcuau gacgauucua agaauaacaa agugcugguu     1920

cugacaagag agaaccagaa uaagggaaac cgcaccccuu uugaguaccu caccuccuuc     1980

cccggcgggg aagacggaga gcgguggaga gcuuuugugg ccugggucca gggcaauaag     2040

gcuuaucgca uggccaaacg gaacagacug cuucgcaaga auuacgggac agaugaaagc     2100

cggggauuca ucgacagaaa ccugaaugau acgcgcuaua uuugcaaauu uuucaagaac     2160

uacguggagg aacaucucca gcuggcggcc cggaccgacg gcgacacagc uagacggugu     2220

gugguuguca augggcaacu gaccgccuuc cuucgcgcua gauggggccu cgccaaagug     2280

cggggagauu cugaccgcca ccaugcccug gaugcugcgg uggucgccgc uugcacacac     2340

ggcaugguga aggcccuggc cgacuacagc agacggaaag agaucgcuuu ucuccaggag     2400

ggguucccug aucccgaaac cggagagauu cugaaccuug ccgcguuuga cagagcucgc     2460

gaacacuucc cugagcccug gccgcauuuu gcccacgaac ugaaggccag acucuucauc     2520

gaugaccugg uugcucugcg ggaggauaug cagcaucuug gcuccuaugc ccaggacgau     2580

cuggaccgcc uccggacgcu guuugugucu agagcgccuc aacgccggaa ugggggagcu     2640

guccacaagg gcacaaucua cgcccagccc gagaaccuca aacagcaggg gagcaccauu     2700

gaaaagaucc ugcuuaccgg ccugaaacuc caagauuucg acaagcugcu gaaucccgag     2760

uccagcgacc acuuugugga accucauaga aacgagcgcc uuuaugccgc uauuagacgg     2820

cgccuggagg aguucggagg caaagccgau aaggcuuuug ggccggacaa gcccuucuac     2880

aaaccugaua agaauggaca gcccacaggc ccuaucguua gaucuauuaa acucgugcgg     2940

gggaagcaga ccggaauccc cauccgcggc gggcuggcca aaaacgacuc caugcucaga     3000

gucgaugugu uuacaaaggc gggcaaguuc caccugguuc cuguguaugu ccaucaccgg     3060

gugacgggac uucccgacag agccauugug gcuuucaaag augaagaaga guggacccug     3120

aucgacgaaa gcuacgccuu ucucuucucu gucuauccga acgauuuugu uugugugcaa     3180

cagcgcggca agccucugau ugagggguac uacgcuggac uggaccgggc caauggcaac     3240

aucaaugugu gggcccauga uagagcugcg uccgucggga aagacggacu uauucgcggc     3300

aucggcguga agacagcccu gagccucgag aaacugaacg uugacgugcu cgggcggauu     3360

uaucccgcuc cucccgaaac cagacgggga cuggccuga                            3399


<210>  3988
<211>  3399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3988
augaagaaaa uccugggcgu ggaccucggg auuaccagcu ucggauacgc cauccugcag       60

gagacaggca aggaucuuua uagaugccug gacaacuccg ucguuaugcg gaauaacccc      120

uacgaugaaa aaucugggga gagcucccaa ucuauucgca gcacgcagaa guccaugaga      180

cggcucaucg aaaaacgcaa gaagcggauu agaugugugg cucagaccau ggagcgcuau      240

ggaauccugg acuacucuga aacaaugaaa aucaaugauc cuaagaacaa uccgauuaaa      300

aacagauggc agcuucgggc cguggacgcg uggaagcgcc cccugagccc ucaagagcuc      360

uuugcuaucu ucgcccacau ggccaaacau agaggcuaua aguccauugc uaccgaggau      420

cugaucuacg aacuggagcu cgaacugggg cuuaaugacc ccgagaagga gagcgagaaa      480

aaggccgaug agcggcgcca ggucuauaac gcucugagac accucgaaga acugcggaaa      540

aaguacggag gcgaaacaau ugcccagacc auccaucgcg cgguggaggc cggcgaccuu      600

agaucuuacc ggaaucacga ugacuaugag aaaaugauua gacgcgaaga uaucgaggaa      660

gagaucgaaa agguucugcu ccggcaggcu gagcuggggg cccugggacu cccugaggaa      720

caaguguccg agcugauuga cgaacuuaaa gcgugcauca cagaccagga gaugcccacc      780

auugaugaaa gccuguuugg caaguguacg uucuacaagg acgagcucgc cgcgccugcc      840

uauucuuacc uguaugaucu uuacagacug uauaaaaagc ucgcugaccu gaacaucgau      900

ggguacgagg ucacccagga agaccgcgag aaagugauug aauggguuga gaagaaaauc      960

gcccagggaa agaaucugaa gaaaaucaca cauaaggauc uccggaaaau ucugggccuu     1020

gcuccggagc aaaagaucuu ugggguggaa gacgagagaa uugucaaagg aaagaaggaa     1080

ccccgcaccu ucgugcccuu uuucuuucug gccgauaucg ccaaauucaa ggagcucuuu     1140

gcuuccauuc agaaacaccc ugacgcgcug cagaucuucc gggaacuugc cgagauccug     1200

caaagaagca agacaccuca ggaggcucuc gaucgccugc gggcccugau ggccggcaaa     1260

ggcauugaca ccgacgauag agaacuccug gagcuuuuua agaacaagcg cucugggacg     1320

cgggaacugu cccacagaua cauccucgag gcucugcccc uguuccuuga aggauaugac     1380

gagaaagagg ugcagcggau ucucggcuuc gaugaccgcg aagauuacag cagauauccg     1440

aagucccugc ggcaucugca ccucagagag gggaaucugu uugaaaaaga ggaaaacccu     1500

aucaauaacc augccgucaa gucucuugcg agcugggcuc ugggacucau ugccgaccug     1560

uccuggcgcu acggccccuu cgaugagauc auucuggaga caacccggga cgcccuuccu     1620

gagaaaauca gaaaggaaau cgauaaagcu augcgcgaaa gagagaaggc ccucgacaag     1680

auuaucggga aauauaagaa agaguuuccc agcauugaua agcggcuggc ucgcaaaauc     1740

cagcuguggg aaagacaaaa gggacucgac cuguacucug gcaagguuau uaaccuuucc     1800

cagcugcucg auggcagcgc cgacaucgag cacaucgugc cccagucucu ugggggacug     1860

agcacagacu auaauaccau ugugacccug aaauccguca acgcggccaa gggcaaucgg     1920

cucccugggg auuggcuggc uggaaacccg gacuaccgcg aaagaaucgg caugcugucu     1980

gagaaagggc ucauugauug gaagaaacgg aagaaucugc uugcccagag ccuggacgaa     2040

aucuacacag agaacacgca cuccaagggc auucgcgcua ccagcuaucu cgaggcccug     2100

guggcgcaag uucugaaaag auacuauccc uucccugauc ccgaacuucg gaagaaugga     2160

aucggcgugc gcaugauccc ugggaaaguc acaucuaaga ccagaucccu ccugggaauu     2220

aaaagcaagu cucgggagac aaacuuucau cacgccgaag acgcucugau ccucuccacc     2280

cugacgagag gcuggcagaa ucgccuucau cggaugcuga gagauaacua cggaaagagc     2340

gaggccgaac ucaaagagcu guggaagaaa uauaugcccc acauugaggg gcugacacuu     2400

gcugacuaca ucgaugaagc cuuccgccgg uuuaugucca agggcgagga aucucuguuc     2460

uauagagaca uguuugauac cauucgcagc aucuccuacu ggguggacaa aaagccgcuc     2520

ucugccagcu cccauaaaga gaccguuuac agcucucggc acgaagugcc uacacugaga     2580

aagaauaucc ucgaggcuuu cgauucccug aacgucauua aggaccgcca caaacuuacc     2640

acagaggaau uuaugaagcg guaugacaaa gagaucagac agaagcugug gcuccaucgg     2700

auugggaaua cgaacgauga aagcuaccgc gcgguggagg agagagccac ccagaucgcu     2760

caaauucuga cacgguauca gcugauggac gcccagaaug auaaagaaau cgacgagaag     2820

uuccagcaag cccuuaagga acugauuacc ucucccaucg aggucacagg caaacuccug     2880

cgcaagauga gauuugugua cgauaaacuc aacgcuaugc agaucgaccg gggacugguu     2940

gaaaccgaua agaacaugcu uggcauucac aucuccaaag ggccuaauga gaagcugauu     3000

uucagacgca uggacgugaa caaugcccau gagcuccaga aggaacggag cggaauccug     3060

ugcuaucuga acgagaugcu uuucauuuuu aauaaaaagg gccugaucca cuacgggugu     3120

cucagaucuu aucuggaaaa aggacaaggc agcaaguaca ucgcucucuu caacccccgc     3180

uuuccugcua aucccaaagc ccagccgucc aaguucacga gcgauucuaa gauuaaacag     3240

gucgggaucg gcuccgccac cggaauuauc aaggcucacc uggaccuuga uggccaugug     3300

cggagcuacg agguuuuugg gacacugccu gaaggaucca uugagugguu caaagaggaa     3360

ucuggcuaug ggagagugga ggacgacccc caccauuga                            3399


<210>  3989
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3989
auggaccuga acuacagaau cggccucgau auugggaucg ccagcguggg augggcuguc       60

cuggagaaua acuccaagga cgaacccguu cggauugugg aucuuggcgu gcgccuguuc      120

gaggccgcgg aagucccuaa aaccggggcu gcccucgccg agccgagacg ggaagcucgc      180

acaacgagac ggcgccugag acggcgcaag cacagacuug agcggaugaa auggcugcuc      240

caggaguaug gacugaucca aaccgaagag uuuaugaagc gcuaccauuc ugccggccug      300

cccgacgugu auagacuccg guacgaaggg cuggagcgca aacuuaagga ugaggaacug      360

gcucagauuc ucauccacau cggaaagcau agaggcuucc ggagcacaag aaaagccgag      420

cugaaggaca aaaaugauaa ggaaaccggc gcgguucuuu ccgccacacg cgagaacgaa      480

aaacugaugg aggagcacgg guaucggacc gcuggagaaa ugauuuacaa ggacgauaag      540

uuuagaacag gcugcaccug gaaagaggaa ggguaucgcc ucacgccucg gaauaaggag      600

ggagacuaca aaaacaccau gcugagagcc cugcucgaag augaggugaa ggucaucuuc      660

gacugucagc gcaaauuugg caaucagaag gcuucugagg aacuggagaa agccuaccuu      720

gcgauuauga caagccaacg guccuucgau auggggcccg gaaagcagcc ugacggcucu      780

gccagccccu augcuaugga aggcuuuggg gacagagugg gacugugcgc ccucgagccu      840

cgcgaaaagc aggagccgcg ggcucccaaa gccgccuaca ccuccgagcu guucguugcg      900

cuucagaaga ucaaccaucu gagaauuaca gagaaaaaug gcaaggggcg cggacucacc      960

agcgaggaac gggagagacu gucugaucug cuccacaaaa agaaggagcu gacguauggc     1020

gcugugcgga aagaacuuca acuggaccuc cgcuacagau uuaacacacu gaauuauucc     1080

agcaagaaaa ugaccgagga agagaagauc aaagaugccg aaaaggcuaa guucgucucu     1140

augguguccc acuacgagua ucggaaaaga augagcggga ugcuugagca gcugucuccu     1200

gaagaacagg ccgaccucau ggaugaagug ggaaagauuc ugacacugua caaaaacgac     1260

gauacccgcu cccgggaguu ucagaagcuc ggccuggaca gcgaaaucac cgaggagcuu     1320

cuggaacuca aucccagagg cuuccaacau gucucccuga aagccaugaa gaagcugaug     1380

ccuuaccuua aagaggggcu cacauaugau aaggcuugug ccgaagcggg auacgacuuu     1440

cagggcucug uucagaaaga gcgcagcaga cugcugaagg ggaaagaaau cacggauauu     1500

aucaacgaca uuaauaaccc cguggugaag cgguccguca gccaaaccgu gaaaguuauc     1560

aacgcuauua uccagaagua uggaucuccg caggccguga auaucgagcu cgcccgcgag     1620

auguccagaa acuacgacga acggcgcaag auggagaaac agaugaagga uagacaaagc     1680

gaaaaugagc gggcuaaaaa ggagauucag gaacugggca acgccuuccc uacagggcag     1740

gacauccuua aauaucgccu guggcacgau caggacggca uuugcaugua cucugcuaag     1800

agaauccccc ucgccgagcu guucaccgga gcguaugaua uugaccauau ccugccuuac     1860

agcaucacau uugaugacuc cuaccggaau aagguccuug ugaccucuga agagaaccgc     1920

caaaaaggca aucggauucc cuaugaauac uucgggagcg auuccgagag auggagcaag     1980

uuugagacgc ucguuaacuc uagaauccgc gacuaucgga aacagcagaa ucugcugaag     2040

caguccuuca cagaagagga aagaaaagag uuuaagcaac gcaaccucaa ugauaccaag     2100

uacauuaccc gggugaucua uaacaugauu agagaaaauc uggcccuugc ucccuacaac     2160

agcccuggca aaaagaaaca ggucuucgcc gugaauggag gggucacagc uuaucugcgc     2220

aagcgguggg gaaugucugc caaagacaga uccaucgaua cccaccacgc gcgcgacgcc     2280

gugguuauug cuuguugcac agacggcaug auccagaaga ucagccggaa cauucagggg     2340

agagagcucc uguacgcccg gggcuuuacg gcuguggaug ucgagaccgg agaaaaguuc     2400

acacgcgacg auuuuaccag agaccaaugg gaugagaucu ucggcgugca gguuccgaaa     2460

cccugggaau acuuuaacga ggaacuggac auucggcuug gggagaaucc uguggaguuc     2520

aucgaaacac auuccgaugu cucucgcaag cucgacuauc ccgccugguu uuacgagaac     2580

aauauuauca gaccuguguu cgugagccgg augcccaaca gaaaaguuac cggagccgcu     2640

cacgcggaua cgauccgcuc cccgcggcau uaucaggaaa ccggcucugu ccugacaaag     2700

accgcccuga uugaccucaa acugaauaag aaaacagggg agaucgagaa cuacuauaag     2760

ccugaaagcg auacccuucu guacaaugcu cucaaggaga gacuggaacu guauggaggc     2820

gacgggaaaa aggccuucga ggaacccuuu uacaaaccua aggccgaugg cucccgcgga     2880

aaccuugugc ggaaagugaa gacguacgac aagcucacac ugggcgucua ugugaauagc     2940

caacacggga uugcugccaa cgcgaaugga ucuaugguua gaguggacgu cuuccgcgug     3000

uccggcaaau acuauuuugu ucccguguac aucgcugaug ucgugcggaa gagacugccu     3060

augaaagccg uugugcaggg gaagcaguau auugacugga aagagaugga ggauaaggac     3120

uucaucuuua gccucuacac ccgcgaucug aucucuuuca aguccaaaaa cggaaagaaa     3180

gucacaugug uggacggcac cagcaaggcc gugaaugaag agauugucua uuacuacggg     3240

gcuaacaucu cuaccgccag cuuuuccggc aaaagccaua auggaucuuu cgaauuuaca     3300

ucccuuggca uucaggggcu ggaguaucuc aagaaguacc aaguugaugu gcugggaaac     3360

gugagcgaag ucaaaagaga gaagcggaug ggcuuccgcu ga                        3402


<210>  3990
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3990
augggcgggu ucgaggugga cagaaagauc agccugggac ucgauauugg cgucuccucu       60

guuggguaca gcgugaucga cguggauacc ggaaaagucc uggaacuugg cucccggcug      120

auuaacggga agaucggaac agagaaucag gcccgcagag aaaugcgggg cacgcgcaga      180

cucauuaacc ggaaaaagca acgcagaaaa gacaccguga agcuguuuaa ugaguucaaa      240

cuuaucgauu cuaaaaagga cgaacacuau uuugaucauu ucgcuaacaa uagcgacccc      300

uacgagcugc ggguuaaagg ccucuccgag aagcugucuc ugagcgaaau cgccgauggg      360

cucuaucaca uugugaaacg cagaggaauc uccuacgacc ugaaggaugc ggacgaggaa      420

agcgagggcu cugaguauga aaacgggcuu aagauuaaua acuccgagcu gaaaaaucag      480

accccugcuc agauccagcu caagcggcug aacgaauacg gagccauucg cggcaaaguc      540

gugaucgaug aggaaaagau ucuucugaau guuuuuccga caaaagccua ucucgacgag      600

gcuaagagaa ucaucgagaa acaacgggag uucuaccccg ggauucugac agaggaauuu      660

gaggaaaagu acugccagau ccugacccgc aagagagacu auuucguggg accuggcagc      720

gagaaaucuc ggacagauua cggcauuuau aaggaggacg ggagaacccu caacaaucug      780

uuugaagaac uuaucggaca ugauaaaauu uaccccgagg aacugcgcgc cuccggcgcu      840

agcuucacgg cccagcucuu uaacguccug aaugaccuua acaaccugca caucucuucc      900

uaugaagauc agaagcucac cacagagaau aaagagcaaa ucauuagcga acugaagucc      960

accacaucua acgugaauau gaugaagcug auccagaaaa uugcggggug ugaggaaagc     1020

gacaucuccg gauaccggac cgauaagaac uauaaaccug agauuagcuc uaugagaauc     1080

uaccgcaagg ugcauaaaaa gcuccuggag uccgaaaucg acauuacgaa uuggcccgau     1140

gacuucauca acaaacuuag cuuuauucug acacucaaua ccgagaacgg cgaaauccgg     1200

aaucagcuga agaacagacu ucagccugag uacgauuucc ugacagacga acucauucaa     1260

accaucauug acaauaagga gucuuucgau aucaaaagca acaauaagug gcaccgcuuu     1320

uccaucaaaa ccauggagcu gcugauuccg gaaaugauca agcggcccgu cgagcagaug     1380

acacucauua acgaaauggg gcugaucaaa aauucuggaa agaaguucag caacuauaag     1440

uaccuuccuu auaagaaaau ugccgaggac aucuacaacc ccguugcuuc caagagcgug     1500

agagaagccc ugaaaaucgu gaaugcuauu cucaaggagu auggccacau cgaguacauu     1560

gucguggaaa ugccucgcga uaagaacgag gacgaagcca aagaucagau cgagaaguuu     1620

caaaaagcga auggccggcu gaaggacgcc gcucuggaag aguucacgga ggccauuggg     1680

gauaaaucua ccguugacaa gaaacuugga gugcauagau ccaagcuccu guuuaagauc     1740

cgccuguggu aucagcagcg gggcauggau cucuacgaag ggaaacccau cagcgcuguc     1800

gaccugcuua acaauccgga uucuuuccag auugaccaca ucauuccuca auccgugagc     1860

uuugaugacu ccuucaacaa uaagacacug ugcaacucug gacucaauca gaucaaagac     1920

aagaagaccc ccuuugagua caugaacgaa ggcuaugggc agggcuucga uagcaugaaa     1980

gccaugguua ugaagaaucc uagaauugac cauaacaaac ggaagaauua ccuguuuucc     2040

gagaacgugu cugauaucga gacacgcaaa agauucauca gccggaaucu ggucgacacc     2100

agauaugccu cccgcguggu ccuuaacaac cuccaggaau uuuucaagga gaaggaaagc     2160

gauacgaaaa uuucuaucau ucggggaaag uucacaucca aucugagaaa acacuggaag     2220

aucaacaaaa aucgcgacac cuaccaccau cacgcuauug augcgaccau cauugccgcu     2280

acacccuuuc ugaacaucug gaagaaaggc agcucuaucu uccccuccaa uguggagagc     2340

gaagacuuuu ucgauauuaa gaccgggaag aucauuggaa acgacgaguu uaauaaauau     2400

gccuacgaug agccuuauaa cggcuucuac aaugaacuca gaaacgccac agaccggauc     2460

aaguuuucuc aucaaauuga uaaaaagaug aaucgcaaag uuagcgacuc cacgaucuac     2520

agcaccagag uggcuaaguc uccgaagaac aaaaaggagu ccgaauauau cgucgccaaa     2580

auuaagaaua ucuacgacgc ggaugaguau gcuaaauuca agcggauuua cgacaaggau     2640

aaaacaaagu uucugcuuaa caaauaugac ccccgcagcu ucaaucaccu ggaaaagauc     2700

augaacaccu acccugauuc cauugagaga acaaugucua acaauaaagu gaagguugug     2760

cggaucagcc ccuuugagcu cuaucgccag gaccaugggc cugucaagaa auacuccaag     2820

aaaaacaaug gacccgaaau caagcagcug aaauaccugg auaagaaggu gggcucuagc     2880

auugacguga ccccggauaa cgccaaaaau aagcacguua uccuuaaauc ccucaagccu     2940

uggagaacgg acauuuaucu gaaccacgag accggggaau acgagaucau gggcauuaaa     3000

uauucugauc ugaaguucaa uaaaggagaa ggcuacggga ucaagaaggc caaauaucuc     3060

gagauuaagc aucgggagaa agucagcgac gacuccgaau uuauguucac acuguacaag     3120

aaaaaccgca ucaaggugga gaauauggaa accgaugaga gcguggagau gcuuuucugg     3180

ucuagaacaa uggacuccca gaagggauau gcugaacuga aacccaucua caaguacaaa     3240

accgaggaug acaacuggcc uauuuauggc aaugccaaga aacaaaucau uaagaagcuc     3300

auccccaaaa acuguaagau uuggaaaguc aauacggaug ugcuggggaa cccuuuuuac     3360

guugaaaagg agggagaaua ucccaaagac gugcuggauu ga                        3402


<210>  3991
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3991
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaaaauu ccggaaaaau ugugcacgcu ucuagcagaa ucuuccccgc cgcgaccgcu      120

gauaacaaug uggaacggcg caagaacaga caggcccggc gccugcauag acggaaaaag      180

caccgcggcg ccagacucaa agaccuguuu gaguacuaug ggcuucugac agauuucucc      240

aaggucucua uuaaucucaa cccuuaccgg cugcgcgugg acggacugga ucaacagcuc      300

acgaaugagg aacuguuuau cgcucuuaag aacaucguua aaagacgggg cauuagcuau      360

cuggacgaug ccuccgaaga cggcgggacc gugucuagcg auuacggaaa ggcugucgag      420

gagaaucgca aacuccuggc cgaacagaca ccgggccaga uccaacuuga gagauucgaa      480

aaguaugggc agcugcgggg agacuuuacc gugguugaga acuccgagaa augccgccuc      540

auuaaugugu ucagcacauc ugcguacaag aaggaagccg agagaauccu gcggaaacag      600

caggaauuua acaaucaaau uaccgaugag uucaucgaag acuaucugaa gauucucaca      660

ggcaaaagaa aguacuacca ugggcccgga aacgagaaau cccgcaccga uuauggccgg      720

uuuagaacgg acggcgcuac ccuggacaau aucuucggga uccuuauugg aaaguguaca      780

uuuuacccua acgaguaucg cgccagcaag gcuucuuaca ccgcccagga guucaaccug      840

cucaaugauc ugaacaaucu uacagucccc accgaaacga agaagcuguc cgaggaacag      900

aaaaagacaa ucauugaaua ugcgaaaagc gccaagaccc ucggcgcuuc uacacugcug      960

aaauacaucg ccaagaugau ugacgcuucc guggaucaga uccgggggua uagaguggac     1020

gucaacaaua agccugagau gcacaccuuu gagguuuacc gcaaaaugca aagccucgaa     1080

accaucuccg ugggagagcu gucucggaac auucuugaug aacuggccca uauccucaca     1140

cugaauacgg agagagaagg cauugaggag gccaucaaca ccaaacuucg cgacagcuuc     1200

ucccaggauc aggugcugga acucguccaa uuccggaaaa auaacagcuc ucuguuuucc     1260

aagggguggc acaauuucag ccugaaacuc augauggagc ugauucccga acuuuacgag     1320

acaucugagg aacagaugac cauccugaca agacucggaa agcaaaagag caaagagacc     1380

uccaagcgca cgaaauauau cgacgaaaag gaggugacag aagagauuua caacccuguu     1440

guggcuaaau cuguccggca ggcgaucaag auuaucaaug aggccaccaa gaaacacggc     1500

auuuuugaua acaucgugau ugaaauggcu agagagaaua acgaagagga cgccaagaaa     1560

gauuauauca agcggcagaa agccaaccag gacgaaaagu acgcugccau ggagaaagcg     1620

gcuuuccagu auaauggcaa gaaggagcug ccggacaaca ucuuucaugg gcacaaagaa     1680

cuggccacca agauucgccu uuggcaucag caaggagaga aaugccucua cacaggcaag     1740

agcaucccca uuuccgaucu gauccacaau caguauaaau acgaaauuga ccacauccug     1800

ccucucagcc ugucuuucga ugacucccuu agcaacaagg uucuggugcu cgccaccgcu     1860

aaucaggaga aggggcagag aacacccuuu caagcccugg auucuaugga cgaugcuugg     1920

uccuaccggg aguucaaaag cuaugucaag gaauccaaac ugcuuggaaa caagaaaaag     1980

gaguaccucc ugacggaaga ggacaucucu aagauugaag ugaaacagaa guuuaucgag     2040

agaaaucugg ucgauacccg cuauagcucc cgggugguuc ucaacgcccu gcaggacuuc     2100

uacaaaaauc auaacuuuga uacaaccauu ucugugguca gaggccaguu cacaagccaa     2160

cuucgcagaa agugggggcu ggagaaaucc cgggaaaccu aucaccauca cgcgguggac     2220

gcccucauca uugcugccag cucucagcug cgccugugga agaagcagaa uaacccucuu     2280

aucgcuuaca aagagggcca auuuguugau ucccagacgg gagaaaucau uagccucacc     2340

gacgacgagu auaaggaacu gguguucaaa gcccccuacg aucauuucgu cgacacacug     2400

ucuuccaaga ccuuugagga uagcauccuc uucucuuacc agguggacag caaauuuaau     2460

agaaagauuu ccgaugcgac aaucuaugcc acccggaaag cuaagcugga caaggagaaa     2520

aaggaauaca cguauacacu uggcaaaauu aaggauaucu acagccuggg gaccaaaaca     2580

ccgucuaaga ccggauucua uaaguuucuc gaccuguaca acaaagauaa gucccaguuc     2640

cugauguuuc aaaaagaccg caagaccugg gaugagguga ucgaaaagau uauggagcag     2700

uauagaccuu ucaaggaaua cgacaaggcc ggcaaacuug uugauuuuaa ucccuucgag     2760

aaguaccggc aggagaacgg gccuauccgc aaauauagca agaaaggaaa cggccccgaa     2820

auuaaguccc ucaaguacua ugacauccug cuggggaaac acaagaauau uacacccgag     2880

ggcucuagaa acacggucgc ucuccugagc cuuaauccuu ggcggaccga cguguacuau     2940

aacauggaaa ccaaaaagua cgaguuucug ggacucaaau augccgaucu gccguucgaa     3000

gagggcgggg ccuacggaau cuccacagag acauacaaug aacugcgcga gaaggaaggc     3060

auugggaaga acucugaguu uaaauucacc cuuuauaaga augaccugau ccucaucaaa     3120

gauacggaga caaacuguca gcaauucuuu agauucugga gccggaccgg aaaggacaau     3180

cccaaauccu uugaaaagca caaaauugag cugaagccuu acgaaaaggc uaaauucgag     3240

aagggcgagg aacucgaggu gcuggggaaa gugcccccuu cuagcaacca guuucagaag     3300

aauaugcaga ucgaaaaccu uuccauuuau aaagucaaga ccgauguucu gggcaauaag     3360

cauuucauca aaaaggaggg agacaaaccc aagcucaaau uuuga                     3405


<210>  3992
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3992
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaaaauu ccggaaaaau uauccacgcu aacucuagac uguuccccgc cgcgaccgcu      120

gauaauaacg uggaacggcg caagaauaga caggcccggc gccucaacag acggaaaaag      180

caucgcggcg ugagauuuca agaccuguuc gaggauuacg ggcuucugac agacuuuagc      240

aaagucucca uuaaucucaa cccuuaucgg cugcgcgugg auggacugaa ucagcagcuc      300

acgaacgagg agcuguucau cgcccuuaag aauaucguua agagacgggg cauuucuuac      360

cuggacgaug cuagcgaaga cggcggggcc guguccucug auuauggaaa agcugucgag      420

gagaaccgca agcuccuggc cgaacagacc ccgggccaaa uccagcuuga gagauuugaa      480

aaauacgggc aagugcgggg agacuucaca guuguggaga auggcgagaa gcugcgccuc      540

auuaacgucu uuagcaccuc cgcguauaaa aaggaagccg agagaauccu gcggaagcag      600

caggaguuca auaaccagau uacagaugag uuuaucgaag acuaccugaa aauucucacc      660

gggaagagaa aauauuacca cggacccggc aacgaaaaga gccgcacaga uuacggccgg      720

uucagaaccg acgggacgac ccuggacaau aucuuuggaa uccuuauugg caaaugcaca      780

uucuauccug aggaauaccg cgcuucuaag gccuccuaua ccgcucagga guuuaaccug      840

cucaaugauc ugaacaaucu uacagugccc accgaaacga aaaagcugag cgaggaacag      900

aagaaaguga ucgucgagua cgccaaggag gcgaaaacac ucggggccuc uacccugcug      960

aaguauauug cuaaaaugau cgacgccucc guugaucaaa uucggggaua cagaguggac     1020

gugaacaaua agccugaaau gcauacauuc gaggucuauc gcaagaugca gagccucgaa     1080

accaucucug ugggcgagcu gucccggaac guucuugaug aacuggcuca cauccucacc     1140

cugaauacag agagagaggg gauggaagag gccauuaaca cgaaacuuaa ggacagcuuc     1200

ucccaggauc aagugcugga acucguccag uuucgcaaaa auaacucuag ccuguucucc     1260

aagggauggc auaauuuuag ccugaaacuc augauggagc ugauccccga gcuuuacgaa     1320

accucugagg aacagaugac aauucugacc cggcucggca agcagaaguc caaagagaca     1380

agcaagagaa ccaaauacau cgacgaaaag gagcugacgg aggaaauuua uaacccugug     1440

guugccaaau cugugcgcca agcuaucaag aucauuaaug aggcgacaaa gaaacacggc     1500

aucuucgaua acauugucau cgaaauggcc cgggagaaca augaagagga cgcuaagaaa     1560

gauuacauua agagacagaa agccaaccag gacgagaaga augccgcuau ggaaaaagcc     1620

gcguuucagu auaacgggaa gaaggagcug ccggacaaua ucuuccacgg acauaaagaa     1680

cuugcuacca agauucggcu cuggcaccaa cagggcgaga aaugucugua caccgggaag     1740

acaaucccca ucagcgaucu gauucauaac caguauaaau acgaaaucga ccacauucuc     1800

ccucuguccc uuucuuuuga ugacagccug uccaauaagg ugcucguccu ggccaccgcc     1860

aaccaggaga agggacaacg cacacccuuc caggcucugg auagcaugga cgaugccugg     1920

ucuuauagag aguuuaaauc cuacgugaag gaaagcaaac uucucggcaa uaagaaaaag     1980

gaguaccugc ugacggaaga ggacaucucu aagauugagg uuaaacagaa guucaucgaa     2040

cggaaccuca ucgauaccag auauuccagc cgcguggucc ugaaugcccu ucaggacuuu     2100

uacaaagcuc acaaguucga uacaaccauu uccgugguuc gggggcaauu uacaucucag     2160

cugagacgca aauggggccu cgaaaagagc agagaaaccu aucaucacca ugcgguggac     2220

gcccugauca uugcugccuc cucucagcug cggcuuugga agaaacaaaa caauagccuc     2280

aucuccuaca aggagggaca guucgucgau agcgaaacgg gcgagauuau cucucugacc     2340

gacgacgagu auaaagaacu gguguucaag gcuccuuacg aucacuuugu ggacacacuc     2400

cgcuccaaaa aguucgagga uagcauccug uuuucuuauc agguugacuc caaauucaac     2460

agaaagauua gcgaugccac caucuacgcg acacggaagg ccaaacuuga caaggaaaag     2520

aaggaguaca ccuauacgcu ggggaaaauu aaggauaucu acucucucgg aacaaaggcu     2580

cccagcaaaa ccggcuuuua uaaguuccug gaccuguaca auaaagauaa gucccaguuu     2640

cuuauguucc aaaaagaccg caagacaugg gaugaaguca uugagaagau cauggagcag     2700

uauagaccgu uuaaagaaua cgacaagacc gggaaacucg uggauuucaa cccuuuugag     2760

aaguaucggc aggaaaacgg acccauccgc aaauacagca agaagggcaa ugggccugag     2820

auuaaaucuc ugaaguacua ugacauccug cucggcaaac auaagaacau uacccccgaa     2880

ggauccagaa auacaguggc ccugcuuagc cugaaccccu ggcggacgga cgucuacuau     2940

aauauggaga ccaaaaagua cgaguuccuc ggccugaagu augcugaucu gccuuuugaa     3000

gaagggggag ccuacggcau cuccacagaa accuauaacg agcuucgcga gaaagaaggg     3060

auuggaaaga acucugaguu caaauucaca cuguacaaga augaccucau ccugauuaaa     3120

gauaccgaaa cgaauugcca gcaaaucuuu agauucugga gccggacagg caaggacaac     3180

ccgaaauccu uugagaagca caagaucgaa cucaaacccu acgagaaggc cagauucgag     3240

aaaggggagg agcugaaggu gcuuggcaaa guuccucccu cuagcaauca gcugcagaag     3300

aacaugcaga uugaaaaucu cuccaucuau aaggugaaaa ccgauguccu gggaaacaag     3360

cacuuuauua aaaaggaggg cgacgaaccu aaacugaagu ucuga                     3405


<210>  3993
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3993
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaaaauu ccggaaaaau uauccacgaa aacucuagac uguuccccgc ugccaccgcg      120

gauaauaacg uggagcggcg caagaauaga caggcucggc gccucaacag acggaaaaag      180

caucgcggcg ugagauuuca agaccuguuc gaagauuacg ggcuucugac agacuuuagc      240

aaagucucca uuaaucucaa cccuuaucgg cugcgcgugg auggacugaa ucagcagcuc      300

acgaacgagg aacuguucau cgcccuuaag aauaucguua agagacgggg cauuucuuac      360

cuggacgaug ccagcgagga cggcgggacc gugccguccg auuauggaaa agcugucaag      420

gagaaccgca aacuccuggc cgaaaagaca cccggccaga uccaacuuga gagauuugaa      480

aaauacgggc agcugcgggg agacuucacc guggaggaga auggcgaaaa gugccgccuc      540

auuaacgugu uuucuacaag cgcuuauaag aaagaggccg aaagaauccu gcggaagcag      600

caggaguuca auaaccaaau uaccaacgaa uuuaucgagg auuaccugaa aauucucaca      660

gggaagagaa aauauuacca cggaccuggc aaugagaagu cccgcaccga cuacggccgg      720

uucagaacgg augggaccac acuggacaac aucuuuggaa uccuuauugg caaauguacc      780

uucuaucccg aagaguaccg cguuagcaag gcgucuuaua cagcccagga auuuaauauc      840

cugaacgacc ucaauaaccu gaccgucccu acggagacaa agaaacuuuc cgaagaacag      900

aagaaagaua uuguggagua cgcuaaggaa accaaaacac ugggggccag cacccuccug      960

aaguauaucg cuaagaugau ugacgccucu guggaucaga uccggggaua cagagucgac     1020

guuaauaaca aacccgagau gcauaccuuc gaaguguauc gcaagaugca aucccuggag     1080

acaaucagcg ugggcgaacu cucucggaau guccuggaug agcuugcgca cauucugacg     1140

cucaacaccg agagagaagg gaucgaggaa gccauuaaua caaaacugau gggaguguuc     1200

ucccaggacc agguucuuga gcuggugcaa uuucgcaaga acaauagcuc ccucuucucu     1260

aaaggcuggc auaacuuuag ccugaagcug augauggagc ucaucccuga acuguacgag     1320

accuccgagg agcagaugac aauucuuacc cggcugggca agcagaaaag caaggaaacg     1380

ucuaaaagaa caaaguacau cgaugagaaa gaggucaccg aagagaucua uaauccggug     1440

guugcuaagu ccgugcgcca ggccauuaag aucauuaacg aagcuaccaa aaaguacggg     1500

aucuucgaca acauugucau cgagauggcc cgggaaaaua acgaggagga ugccaaaaag     1560

gacuauauua aaagacaaaa ggcuaaucag gaugaaaaga acgcggccau ggagaaggcu     1620

gccuuucagu acaauggaaa gaaagaacuc cccgacagca ucuuccacgg ccacaaggag     1680

cuggccacaa aaauccggcu guggcaucag caaggggaaa agugccuuua uaccggcaaa     1740

aacauuccua ucucugaccu cauucacaau caguacaagu augagaucga ucauauucug     1800

ccccugagcc ucuccuuuga cgauucucug agcaacaagg ugcuuguccu ggcuacagcc     1860

aaucaggaga aaggacagcg cacgccuuuc caagcgcucg acuccaugga ugacgcuugg     1920

agcuacagag aauuuaaguc uuacgugaaa gauuccaagc ugcugagcaa caaaaagaag     1980

gacuaucuuc ucaccgagga agauaucucu aaaaucgagg uuaagcagaa auucauugag     2040

cggaaucugg uggacacaag auacuccagc cgcgucgugc ugaacgcccu ccaggauuuu     2100

uauaaggccc acaaauucga caccacaauc uccguugugc gggggcaguu uaccucucaa     2160

cugagacgca aguggggccu ugaaaagagc agagagacgu accaccauca cgcugucgau     2220

gcccugauua ucgcugccuc cucucagcuc cggcugugga aaaagcagaa uaacccccug     2280

auuaccuaua aagaaggaca auucguggac agcgagacag gcgaaaucau cucccuuacc     2340

gacgaugagu acaaggagcu cguguucaaa gcgccguaug accauuuugu ugauacacug     2400

cgcagcaaga ccuucgaaga cucuauucug uuuuccuacc aggucgauag caaauucaau     2460

agaaagaucu cugacgccac gauuuacgcu acacggaagg ccaaacucga uaaggagaaa     2520

aaggaauaua ccuacacacu ggggaaaauc aaggacauuu auucccuugg aaccaagacc     2580

ccuagcaaaa caggcuuuua caaguuccug gaucucuaua acaaagacaa gucucaguuu     2640

cugauguucc agaaagaucg caagacgugg gacgagguga ucgaaaagau cauggagcaa     2700

uacagacccu uuaaagagua ugauaagacc gggaaacugg uggacuucaa cccuuuugaa     2760

aaguaccggc aggagaaugg acccauuugu aaauacagca agaagggcaa cgggcccgaa     2820

aucaaauccc uuaaguauua cgacauucuc cugggcaaac acaagaauau cacaccugag     2880

ggaagccgca acaccgucgc ucugcucucu cugaauccgu ggagaacaga uguguauuac     2940

aacauggaaa ccaaaaagua ugaguuccuu ggccugaagu acgccgaccu ccccuuugag     3000

aaagggggag cguauggcau uuccacggaa acauacaaua agcugcggga gaaagaaggg     3060

aucggaaaga acagcgaguu caaauucacc cuguacaaga augaucuuau ucugaucaaa     3120

gacaccgaga caaacugcca gcagaucuuu cgcuucuggu ccagaaccgg caaggauaau     3180

ccuaagucuu uugaaaaaca uaagauugag cucaaacccu augaaaaggc ccgguucgag     3240

aaaggggagg aacuggaggu ucucggcaag gugccuccca gcuccaacca auuucagaag     3300

aauaugcaga ucgaaaaccu gucuauuuac aaagucaaga cagacgugcu uggcaaaaag     3360

cacaucauua aaaaggaggg agaugaaccg aagcugaaau ucuga                     3405


<210>  3994
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3994
augaaguaca ucgagagcgu guccauuggc uucgacaucg gggucggauc uguuggcuau       60

gccgugauua acagcaaaac caagcagauc cuggaauccg gggugucuau uuuucccgcu      120

gccgucgcgc agaaaaaugu ggagagacgg gaaagccgcc aguccagacg gcuccgcaga      180

cggcgcguua acagacugaa ugaucuuaag aaacugcuca agaagaacgg auucaucccu      240

ucuccgaaua aaaaccccua cgagauccgg gugcgcggcc uggaacacca gcuuagcaga      300

gacgagauug cuaucgccgu ccugcauauu gccaagcggc gcgggaucuc cuauagccuc      360

gaugacgugg uugaugagaa ugugacagac uuuaaaggaa agauugcuaa aaaccaggaa      420

gcccugaaga auaagacgcc uggcgagauc caauacgaaa gacugcucaa auauggcaag      480

guccggggga ccgugacaaa cuacguggag uaugaugagc agcccgaaua cuuccugaau      540

gucuuuccua acucugcuua cguugacgag gccaaacgca uucuuaccug ccagaagcag      600

uauuaccccg aaaucacaga ugaccugauc gagaauauug ugaaaaucau uuccagaaag      660

cgggcguauu acaucggacc uggcaacgag aaaagcagaa ccgauuaugg gauuuaccgc      720

acaaauggag aaacccucga gaacaucuuc gaaauccuga uuggcaagga cucugugaac      780

ccggaugagc uucgggcccc cgcuaauucc cugacggccc aagucuauaa ccuccugaau      840

gaccugacca acaucagaua caaugggagc gaacgccuca caccugagca gaagcaggac      900

auucugucug aacuucugac cacagagaaa accgugcaga ugcucaacgu uaucgcuaag      960

aaagugaaug ucaagaaaga ggaaauuucc ggauuucgga cgaagaagga ugacaaaccc     1020

gauuuccaca cacuggaggu guacagaaag guucgcaaac auaugcuuaa caagggcauc     1080

gacaucaaug aauggccuac cgaguguuuu gaugaacugg ccuucauuuu uaaccucaau     1140

acagagaucg gcgagauucg gaacagaauc agcgaaaaag ugguccccaa guaugacuuc     1200

auuaccaauc aaaucgugga ugaccugcug gaggauccga agauuuuugc gaucuccacc     1260

ucucagaaau ggcaccgcuu cagcaucaag acaaugaacg uccucauucc ugaacugcuu     1320

aauggguaca acgagcagau gacgauccug acccggaugg gacuccugga ggacuccaaa     1380

aacgauuaua gcaauauuga caagaucgac auuaaaaacg ugacagaaaa uaucuacaac     1440

cccguuguga gaaagucuau caagaaaugc cuuaagauuu ucaaugagcu guauaaaaag     1500

uacccuaaca ucuccgaugu cgugauugaa cucccccgcg aggacaaucc cgaagaugcc     1560

cggaaacuga ucaagaaaau gcaaaaggag aaccugguug agaaggacac cagccucgag     1620

gcuuuuagaa aagaagccaa ucugucugaa aacggccuua uugauuuccu gcggaagggg     1680

aaaaaucgcg cucucaagau cagacugugg uaucagcagg agggaaaaug ucuguacaca     1740

ggcgaaccua uccuugccga ggaccucugc aagaacgagg ccaauuacga uauugaccau     1800

aucauuccga ucagcguguc cuuugaugac ucuauuaaca auaagguccu guguagcucc     1860

cacuucaacc gguuugaaaa agagaagaaa acccccuucg aauauuuuag caacaagucu     1920

ucccaccugg ggagauacag caauuucgau gaguuucgcg cuucugugaa cgcgaauaaa     1980

cggaucuccg acgugaagag aaagaaccuc cugagcaaag aacuucuguc cgaucucgac     2040

acgcgcaaga aauucaucgc cagaaaucug guugacacac gguaugcuuc ucgcgucgug     2100

uuuaacgagc ugacccaguu cuuugccagc aagaaagccg auaccaaggu ggcugucauu     2160

agaggaaagu ucacauccca acuucggcgc gccuggcagc ucaauaaauc uagagacacc     2220

uaccaucacc augcggugga ugcugcccug aucggcguua caccuuuccu gaagauuugg     2280

cggagcggca aaacgaucua uccccgcaag augaccgagg cccagacaga cuuugaacuc     2340

aacgagauua ccacaaauga ugaguucaaa gaauccuacu aucagcugcc uugcccccaa     2400

uuucuugacu accugaccca gaugcaggag aaccucaaga ucaauuucga aguggauaaa     2460

aagaugaaca gaaagauuag cgacgcuaug aucuacucuu cccggaaaaa gaaacugagc     2520

aaggauaaaa aggagaagga auauucucug gggacgauca augacauuua cuccgaugcc     2580

ggauauagca aauuuaagaa caucuacaaa aaggacaaag agaaguuccu uauguaucac     2640

cucgaucaca agucuuuuga aauucuggag gacaucauua gccaguaccc ggacaaagag     2700

gaauauaucg auuccgaggg caaggucaaa caacgcgcug ugagcccuuu cgaaaucuac     2760

agacggaagu acggguauau uagaaaauac ucuaagaaga auaacggacc caucauuaaa     2820

aacaugaagu auuacaauga gaaccugccu aaaaucuaua uugacaucac ccccaagcag     2880

uccaucgaua aaaagguuua ccucacaaau auuagcccuu ggcgcaccga cguguauuac     2940

aaccgguccc aaaauucuua ccauaucaug ggcauuaagu auagcgaucu gagauacauc     3000

gacgggaaau auggcauucc caagaacguc uacgaaaaga ucaaacuucg cgagaaaauu     3060

ccguccgagu cugaauuuug uuucagccug uauaagaaug aucgggugaa aaucaucgac     3120

ccuaacaaga augaggaaau ugagcucaga uuuaacuccc gcacacugcc cucuguucug     3180

aauuacgugg aguucaagcc ccuuuuucag gaaagcuaua acucccucac ccugcugccu     3240

gucuucagca aaacgacauc ugagggacgg aucauuaaga aauucaccag agaagauaau     3300

aucguguaca aggugaacac agacgagcuc ggcaauccgu acuauauucu gaaagaaucc     3360

caaaacccca agaauaucau cgauaacagc aacaagaccg aguga                     3405


<210>  3995
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3995
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgagggcgu cgggauccuu       60

gaaaagaacu ccggaaaaau uauccacgcu aauucuagac uguuccccgc cgcgaccgcu      120

gauaauaacg uugagcggcg caagaauaga caggcccggc gccucaacag acggaaaaag      180

caucgcggcg ugagauuuca agaccuguuc gaagauuacg ggcuucugac agacuuuagc      240

aaagugucca uuaaucucaa cccuuaucgg cugcgcgucg auggacugaa ucagcagcuc      300

acgaacgagg aacuguucau cgcccuuaag aauaucguga agagacgggg cauuucuuac      360

cuggacgaug cuagcgagga cggcggggcc guuuccucug auuauggaaa agcuguggag      420

gaaaaccgca agcuccuggc cgagcagacc ccgggccaaa uccagcuuga aagauuugag      480

aaauacgggc agguccgggg agacuucaca gugguugaga auggcgaaaa gcugcgccuc      540

auuaacgugu uuagcaccuc cgcguauaaa aaggaggccg aaagaauccu gcggaagcag      600

caagaguuca auaaccagau uacagaugaa uuuaucgagg acuaccugaa aauucucacc      660

gggaagagaa aauauuacca cggacccggc aacgaaaaga gccgcacaga uuacggccgg      720

uucagaaccg acgggacgac ccuggacaau aucuuuggaa uccuuauugg caaaugcaca      780

uucuauccug aagaguaccg cgcuucuaag gccuccuaua ccgcucagga auuuaaccug      840

cucaaugauc ugaacaaucu uacagucccc accgagacga aaaagcugag cgaggagcag      900

aagaaaguga ucguggagua cgccaaggaa gcgaaaacac ucggggccuc uacccugcug      960

aaguauauug cuaaaaugau cgacgccucc gucgaucaaa uucggggaua cagaguugac     1020

gugaacaaua agccugagau gcauacauuc gaaguguauc gcaagaugca gagccucgag     1080

accaucucug ucggcgaacu gucccggaac gugcuugaug agcuggcuca cauccucacc     1140

cugaauacag agagagaagg gauggaggaa gccauuaaca cgaaacuuaa ggacagcuuc     1200

ucccaggauc aaguucugga gcucgugcag uuucgcaaaa auaacucuag ccuguucucc     1260

aagggauggc auaauuuuag ccugaaacuc augauggagc ugauccccga acuuuacgag     1320

accucugagg aacagaugac aauucugacc cggcucggca agcagaaguc caaagaaaca     1380

agcaagagaa ccaaauacau cgacgagaag gagcugacgg aagagauuua uaacccuguc     1440

guggccaaau cuguucgcca agcuaucaag aucauuaaug aagcgacaaa gaaacacggc     1500

aucuucgaua acauugugau cgagauggcc cgggaaaaca augaggagga cgcuaagaaa     1560

gauuacauua agagacagaa agccaaccag gacgaaaaga augccgcuau ggagaaagcc     1620

gcguuucagu auaacgggaa gaaggaacug ccggacaaua ucuuccacgg acauaaagag     1680

cuugcuacca agauucggcu cuggcaccaa cagggcgaaa aaugucugua caccgggaag     1740

acaaucccca ucagcgaucu gauuuauaac caguacaaau augagaucga ccauauucuc     1800

ccucuguccc uuucuuuuga ugacagccug uccaauaagg uccucgcccu ggccaccgcu     1860

aaccaggaga agggacaacg cacacccuuc caggcccugg auagcaugga cgaugcuugg     1920

ucuuacagag aauuuaaauc cuacgugaag gaccuuaaaa gccucucuaa uaagaaaaag     1980

gauuaucugc ugacggagga agacaucucc aagauugagg ucaaacagaa guucaucgag     2040

cggaaccucg uggauaccag auacagcucc cgcguugugc ugaaugcccu ucaggacuuu     2100

uauaaaucuc accaauucga uacaaccauc agcgucgugc ggggccaguu uacaucccag     2160

cugagacgca agugggggcu cgaaaaaucu agagagaccu accaccauca cgcgguugac     2220

gcccugauua ucgcugccag cucccaacug cggcuuugga agaagcagaa caauccucuc     2280

auuacguaua aagaaggcca guucguggac agccagaccg gagagaucau uucucugaca     2340

gaugacgaau acaaggagcu ggucuucaaa gcucccuaug aucauuuugu ggacacccuc     2400

uccagcaaga cauucgagga uucuauccug uuuuccuacc aaguggacag caaauucaac     2460

cgcaagaucu cugaugccac cauuuacgcg acgagaaaag ccaaacuuga caaggaaaag     2520

aaggaguaua cauacacccu gggcaaaauc aaggauauuu auagccucgg gacaaaaacc     2580

ccguccaaga ccggauuuua caaguuccug gaccuguaua auaaagauaa gagccaguuu     2640

cuuauguucc agaaagaccg gaagacaugg gaugaaguua ucgagaaaau uauggaacag     2700

uaccgcccuu uuaaggagua ugacaagacg ggcaaacucg ucgacuucaa ccccuuugag     2760

aaguacagac aagaaaacga gccuauccgg aaauacucua agaaagggaa uggacccgaa     2820

aucaaguccc ugaaguauua cgauauucug cucggcaaac acaagaacau cacccccgag     2880

gggagccgca auacaguggc ucugcuuucc cugaacccuu ggagaaccga cguguauuac     2940

aauauggaaa ccaaaaagua ugaguuccuc ggccugaaau acgccgaucu gccguuugag     3000

gaaggaggcg cuuaugggau uucuacagag acguacaacg aacuucggga gaaggaggga     3060

aucggcaaga auagcgaguu caaauucaca cuguacaaga acgaccucau ucugaucaaa     3120

gauaccgaaa ccaauugcca gcagauuuuu cgcuucuggu ccagaacagg gaaggacaac     3180

cccaaaucuu uugaaaagca uaaaaucgag cucaagccuu augaaaaggc ccgguucgag     3240

aaaggagagg aacuggaggu ccuuggcaag gugcccccua gcuccaauca gcugcagaaa     3300

aacaugcaaa ucgaaaaucu cucuauuuac aagguuaaaa ccgaugugcu ggggaacaag     3360

cacuuuauca agaaagaggg cgacaagccc aaacugaagu ucuga                     3405


<210>  3996
<211>  3408
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3996
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggagcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcagag ugcggcugaa ucaccuuuuu gaagagagcg ggcugaucac cgacuucaca      240

aaggucucca ucaaccucaa uccuuaccag cugcgcguga aaggacugac cgcggaacuc      300

ucuaacgagg agcuguuuau ugcucuuaag aauaugguua agcauagagg caucagcuau      360

cuggaugacg ccuccgauga cggcaacucu agcguggggg auuacgccca gauugucaaa      420

gaaaauucca agcagcucga gacaaaaacc ccgggacaaa uccagcugga acgguaucag      480

aaguacggcc agcuucgcgg ggacuucaca guggaggagg auggaagaaa acaccggcug      540

auuaacguuu uucccaccag cgcuuaucau gccgaagcuc uccgcauccu gcaaacgcag      600

caggaguuca auccucagau uaccgacgaa uuuaucaacu cuuaccugga gauccucaca      660

ggcaagagaa aguauuacca cgggcccgga aaugaaaaau cccggaccga uuacggcaag      720

uauacaacca aaaaggacgc ccaaggccag uacauuacgc ugaacaacau cuucgggauu      780

cuuaucggaa aaugcacauu uuauccugag gaguacagag cggccaaggc uagcuauacc      840

gcccaggagu ucaaucugcu caacgaucug aauaaccuua cagugcccac cgagaccaaa      900

aagcugucug aagaacaaaa guaccagauu aucacauaug ucaaaaauga aaaggcuaug      960

ggcccugcca aacucuuuaa guacaucgcg aaacugcugg ccugugacau ugcugacauc     1020

aagggguacc gcauugauaa guccgacaaa gccgagaucc acacguucga ggcuuaucgg     1080

aagaugaaaa cccucgaaac aauugauauc gagcagaucg aaagagagaa gcuggacaaa     1140

cuugccuacg ugcugacccu caacacagaa cgcgagggca uucaggaggc ccuggaucau     1200

gaauuugcug acggaaccuu cagccaagaa cagguggaug aacuugucca guuccggaag     1260

gccaauucuu ccaucuuugg gaagggaugg cacagcuucu ccguuaaacu gaugauggag     1320

cucauuccgg agcuguaugc gacgucugag gagcagauga caauccugac cagacucggc     1380

aagcaaaaga ccacaagcuc cagcaacaag accaaauaca uugacgaaaa gcagcugaca     1440

gaggaaaucu auaaucccgu gguggcuaag ucuguccgcc aggccauuaa aaucgugaac     1500

gccgcuauca agaaauacgg cgauuuugac aauauuguua ucgagauggc ccgggagacg     1560

aacgaagaug acgagaagaa agcgauucag aagauccaaa aagcuaauaa ggccgaaaag     1620

gacgccgcua ugagaaaagc cgcuaaccag uauaauggga aggccgagcu uccucauucc     1680

guguuccacg gacacaaaca gcuggcgacc aagauucgcc ucuggcauca gcaaggcgaa     1740

cggugccugu acacagggaa aaccaucagc auccacgauc ugauuaacaa ccccaaucag     1800

uuugagaucg accauauucu uccucucucu aucacauucg augacagccu ggccaacaag     1860

guccuggugu acgcuaccgc caaucaggag aagggacaaa gaacgcccua ucaggcucuc     1920

gauuccaugg acgaugccug gucuuuucgc gaacugaaag cguucguucg ggagagcaag     1980

gcccuuucca acaaaaagaa agaauaccug cucaccgagg aagacauuag caaguuugau     2040

gugagaaaga aauucaucga gcggaaucug gucgacacac gcuaugcuuc uagagugguc     2100

cugaacgccc uucaggagca cuuuaaggcu cauaaaaucg auaccaaggu guccguugug     2160

cggggccagu ucacaagcca acucagacgc cacuggggga uugacaaaac ccgggauacg     2220

uaccaccauc acgccgucga cgcccugauc auugcugcgu cuucccagcu gaaucucugg     2280

aagaagcaga aaaacacacu ggugagcuau uccgaagacc agcuucugga uaucgagacc     2340

ggcgaacuca uuucugacga ugaguacaag gagagcguuu uuaaagcccc guaucaacau     2400

uucguggaca cacugaaguc caaagaguuc gaagauucua uccuguuuag cuaccagguc     2460

gacuccaagu ucaauagaaa aaucagcgau gcuaccauuu acgccacccg ccaggccaag     2520

gugggaaagg acaaaaagga ugaaacauau gugcuuggca aaaucaagga cauuuacgcu     2580

cucgggacga aaaccccuuc uaagacagga uuuuauaagu uccuggaucu guacaacaaa     2640

gacaaguccc aguuucucgu uuaucaaaaa gauagaaaga ccugggacga ggucaucgag     2700

aaaauuaugg aacaguaccg gcccuucaag gagagcgaca agaauggcaa agaaguggau     2760

uuuaacccuu ucgagaagua ucgcaucgaa aaugggccca uuagaaaaua cucucggaag     2820

ggaaacggcc ccgagaucaa aucccugaag uacuaugcca gcaaacuugg gaaucacauc     2880

gacauuacac cuaaagauuc uaacaacaag gugguccugc agagcguguc cccguggcgc     2940

gcggacguuu acuuuaauaa aaccacgggc agcuaugaaa uccucggacu gaaguacgcu     3000

gaucugcaau ucgagaaagg cacagggacc uauagaauuu cugaggaaaa guacaacgac     3060

aucaagaaaa aggagggagu ggauuccgac agcgaauuua aauucacccu uuauaagaau     3120

gaucuccugc ugauuaaaga cacagagacc aaggaacagc ggcucuuucg cuuccugucc     3180

agaucuaugc ccaacaaaaa gcacuacguc gagcuuaagc cuuacagcaa agauaaguuc     3240

gagaaaaaug aaucccugau cgagauccuc ggcucugccg acaagagcgg gcgguguauu     3300

aaaggacugg ccaaguccaa ccugucuauc uuuaaggugc gcacagacgu ucuuggcaau     3360

cagcauauua ucaaaaacga aggggauaag cccaaacugg acuucuga                  3408


<210>  3997
<211>  3408
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3997
augggcguga ucuuccugua cgagaacauu gucaagcccu uuccuagcag auaugacaaa       60

gccaucauga agcggcucaa agggcgccag accaugggau acauucuguc ccuugauauc      120

ggcauugcuu cuugcgggua uagcguucug gaaacagacg cccucggagu gccguacaag      180

aucaaaacgc uuaauuccau cauuuucacc agagcggaga accccaagac aggcgcuucu      240

cuggccgaac cucggcgcaa guauagaggg gcccggcgca gaaaacggcg caccagcuuu      300

agaaagcacc ggacacgcca acuguucauc accuccggac uccugucuaa acaggauauu      360

gcuagauacc ugaaucagca ggagccccau gacgauaucu gggaacuccg ggugaagggc      420

cuggacgagc cucuuacaaa ccugcaacuc uuucaggucc uguauuacuu cgugacccac      480

cgcggcuuua aaagcaauag aaaggccgag cuuaaggaua aagacauugg gcccguucug      540

aacuccauca augcuuucaa ggaagagaug cagagccaug auuaccggac gauuggagaa      600

cucuaugcca acgacccuag auuucgcgau cggaaacaca auaaggacua caccaacggc      660

uuccugggga caauccagag aucuuggcug guggaugaga ucaaaaugcu ccugaccaau      720

caacaguccu uuggaaacac acagcuuacc agcgaguuca uugaacugua ucucaauauc      780

uuuacgucuc agcgcaacuu cgacgacggc ccggcguccg ggcccuacag cggagaucug      840

auugacaaga uggucggccc ugauacauuc aaucggucua gaaaacgcgc ccccagagcu      900

accuauacau uucaauacuu cuccuuucuu agcaagcuga acucccucag aaucgccccu      960

ucuauuagcg cucccuucga gacccugacc guggaccagc gcgcccugau cacaaagcgg     1020

cucuggggcc cggauguuaa auccgcggac cugaaguuua aacagaucag aauggugcuu     1080

agccugccug aaacggcccg cuucaaccuc gucaauuaug ggaacacccc cgagaaggcu     1140

cuggaaauug aggauaaaac aaccuuuauc ucuauuaagc cucuucggga gaucgaaaag     1200

gcccugacag gaaccacgcu ccccgugucc caugaccaac uggaugcuau ugccagaauc     1260

cugacagugu acaaaagcga cucuaaucgg aagaccgccu uccacaacca gaccguccuc     1320

agcgaugagg uuaucgaaca gcugcuuccc cugaauuuuu ccaaauucgg ccaccucucu     1380

guggagacaa ugcaggaaau uaucccuuac cuggagcaag ggauggugua uagcgacgcu     1440

gcgaccgccg cuggauacga cuuucgcaac aaucagauug auauggagua uaucagagac     1500

aacgucacaa auccgguggu ucggagagcc guguccgucg ccaacaagcu ggugaaagcu     1560

guugugcgcc gguacggcaa gcccgauagc auucauaucg aacuugccag agaccucucu     1620

cagaauuucc aggagcgcag acaagaugaa guccggcagc agaagaacgc gcgcgacaau     1680

gagaaaauug cuaagagacu ggaagaacug gggaucccug ugaacggagu caauaucacg     1740

aaaaugaagc ucuaugauga gcagcacggc auugaccugu acgaucuuuc caccaacgcc     1800

agcauggccg ugacagacau cuuuucugau gaccgguaug aaauugauca uaucauuccc     1860

uacuccguua gcuucgacga cuccuacacc aacaaagugc uggucucugc uaaggccaau     1920

cgcgagaaga gagaucggau cccuauggaa guguucggca acgacagcga gcgcagacgg     1980

caauuugaga aucgcguuaa cacaaauauc accaaccucc ggaaaaagcg gaaucugcug     2040

aaagaaacgu ucuccgauga ggacaaggcu aaauggaagu cuagaaacau uaaugauacc     2100

cgcuaucuua accggauccu caugaauuac cugagccaga acguggaauu uacagacguc     2160

cuguccgaug ccaaacacgu gauugcgcuc aauggggccg ugaccgcuaa gaucagaagc     2220

cgcuggggaa uuacaaaggu ucgggccgcu ggcgaccugc accaugccgu cgaugcggug     2280

gugaucgggu gugucaccga cgcccuuauc caggcuguga cgagauaugc caaauaccag     2340

gagcgccggg auaacaaggc ugccgccgaa caagaccugg cugagaauaa ccccgaggaa     2400

cucgaugcga aacaguucag aaagguuuuu aacgacuauc cgcugccuug gcccgaguuc     2460

cgcgaggagc ugaugggacg ggugucuucc gacccuagcc agauuauggc cggcagacaa     2520

uggucucacu acacagcuga agagaucucc gagcuuaaac agcccuuugu cgugcggguu     2580

ccuaaucauc gcauuguggg gcccgccacc cucgauacag ucuauggcca gccgaacccu     2640

gacgauggac ugaucaccca gagagugcgg auuaccgacc ugaagcccca aaagaauaaa     2700

gauggcgaag acaucaucaa ggccggggcu aacguuuacg agcucgccgc ggauggaggc     2760

aauaaacugg uguacgaaag cauucuugac gcucugcagg ucgccggggg agauggcaag     2820

aaagccuucc ccacagggcg ccuccuggug agaucuccua uccaggacau uccgguggag     2880

cgggucagag uuacgagcaa ggcuacccug guggugcccg ucggcccuaa guccguggcc     2940

gcgaacggaa gcaugguucg caucgaugug uauaaaacac ccaagaaaua cgucuuugug     3000

ccuauuuaug uucgggacac cgugaaggcu gaacuucccg acagagccgu cgccccugug     3060

aaacccuacu cucaguggua ucaacuccag ccgaaggauc aguuccuguu uucccuguuc     3120

gcuaaugacc ucauccgcuu uaagcacccu cggggcguua gcacaaaaga gauucugacc     3180

gauggguccc aggagaaggg aaaaagaacg gacguggucg gcuacuucac aggggccgau     3240

aucgcuaccg ccucuaucgc gauugaagga cccgaccgca gcuucuccgc ccggggcauc     3300

ggguuugcuu cucuuaagaa cguggagaaa cugaccguga auuauuucgg cgaacuccau     3360

agcguccacg agacacaacc uaugcaguuu agaggacagc gcggcuga                  3408


<210>  3998
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3998
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaggaua ccggaaaaau uauccacgcu uccucuagac uguuccccgc cgcgacagcu      120

gacaauaacg uggaacggcg cagcaauaga cagggccggc gccucaacag acggaagaaa      180

caucgcuccg ugagacugca agaucuuuuu gagucuuacg ggcugcucac ggacuucagc      240

aagcugucca uuaaucugaa cccuuaucag cuccgggucc agggaaugga aaaucagcug      300

accaacgagg aacuuuuuau cgcccugaaa aauaucguga agcgcagagg cauuucuuac      360

cucgaugacg ccagcgagga uggcgggaca guuuccagcg acuauggaaa ggcuguggag      420

gaaaaccggc gccugcuuac cgagaaaaca ccgggccaaa uccagcugga aagauucgag      480

aaguacgggc agcuccgggg agauuuuacc gucgaggaaa auggcgagaa acgcagacug      540

auuaacgugu ucucuacauc cgccuaucgg aaggaagcug agagaauccu gcgcaaacag      600

caagaauuua auaacaagau uaccgacgag uucaucgagg auuaccucac gauucugacc      660

gggaagcgga aauauuacca cggacccggc aaugaaaaga gcagaacaga cuacggccgc      720

uuucggaccg augggacaac ccuugacaac aucuucggaa uccugauugg caaaugcacg      780

uuuuauccug aggaauacag agccucuaag gcguccuaua cagcccagga guucaaccuc      840

cugaaugacc uuaacaaucu gaccguuccc acagaaacca aaaagcucag cgaggagcag      900

aaaaagacca ucauugaaua cgcuaagucu gccaaaacac ugggggcuuc cacgcugcuc      960

aaguauaucg ccaaaaugau ugaugcgagc guggaccaga uccgcggaua ccgggucgau     1020

gugaacaaua agccugagau gcauaccuuu gaaguguaua gaaaaaugca aucccuggag     1080

acaaucaagg ucgaagaacu uccccgcaag guucuggacg agcucgccca cauucugacc     1140

cuuaacacag aacgggaggg caucgaagag gcuauuaauu cuaaacugaa ggauaccuuc     1200

aacagagacc aggugcucga gcuggugcag uuccgcaaaa auaacagcuc ccuguuuagc     1260

aagggguggc auaauuucuc ucucaaacug augauggaac uuaucccuga gcuguacgaa     1320

acguccgagg aacaaaugac aauucucacc cggcugggaa agcagagaag caaggagacc     1380

ucuaaacgga caaaguacau cgaugagaaa gaacugaccg aggaaaucua uaacccgguc     1440

guggccaaga gcguucgcca ggcuauuaaa aucauuaaug aggccacaaa gaaguacggc     1500

aucuuugaca acauugugau cgaaauggcc agagagaaua acgaggaaga ugcuaaaaag     1560

aacuauauua aacagcaaaa ggcgaaucag gacgagaaaa acgccgcuau ggaaaaggcc     1620

gccuuccagu acaauggcaa aaaggagcuu cccgauaaca ucuuucacgg gcacauggaa     1680

cucgcuacga agauccggcu guggcaucag caaggagaga aaugucugua uaccggcaag     1740

aauauucaga ucuccgaccu cauucacaac caguacaaau augagaucga ccauauucug     1800

ccucuuucuc ugagcuucga ugacucccuc agcaauaagg uccuggugcu ggccacagcg     1860

aaccaggaaa aagggcaaag aacccccuuu caggcucuug auucuaugga cgaugccugg     1920

uccuaccgcg aguucaagag cuacguuaag gaaucuaaac uccugggaaa uaagaaaaag     1980

gacuaucugc ucacagagga ggauaucucc aaaaucgaag ugaagcagaa guuuauugag     2040

aaaaaccugg ucgacacccg guacagcucc agaguggucc uuaaugcccu gcaggauuuc     2100

uauaagaaac accaauuuga cacgaccauc ucugugguuc gcggccaguu cacaagccag     2160

cucagacgga aguggaaacu ggaaaagucc cgcgauaccu accaccauca cgcuguggac     2220

gcccugauua ucgcugccuc uagccaacuu agacucugga agaaacauaa caauccucug     2280

auuuccuaua aggaugggca guuugucgac agcgagacag gcgaaaucgu gucucugucc     2340

aacgaggagu acaaagaacu cguuuucaag gcgcccuaug accacuucgu ggauacccug     2400

cggagcaaaa aguuugagga cucuauccuu uucuccuacc aggucgauag caaauacaau     2460

cgcaagauuu cugacgccac gaucuaugcu acaagaaagg ccaaacugga uaaggacaaa     2520

aaggaauaca ccuauacacu cggaaaaauu aaggauaucu acgcucuggg caccaagacc     2580

ccgagcaaaa caggguuuua uaaguuccug gaccuuuaca aaacggauaa gucccaguuu     2640

cucauguauc aaaaagaccg gaagaccugg gaugagguga uugaaaagau caucgagcag     2700

uaccgcccuu ucaaagagua cgacaagaac ggaaaagaag uggauuuuaa ccccuucgag     2760

aaguauagaa uugaaaaugg cccuauccgg aaauacagcc gcaaggggaa cggaucuguu     2820

auuaagucca ugaaauauua cgacaaucug cugggcaagu uugucgacau cacacccagc     2880

gaguccaaaa accccguggc ccuccugucu cuuaauccuu ggagaaccga uguguauuac     2940

aacccggaaa cacggaagua ugaguuccug gggcucaaau acgcggaccu gugcuuuggc     3000

aagggaggca gcuacgggau uuccgagguc aaguauaaua aaaucagaga aaaggaggga     3060

auuggcaaag aaucugaguu caaguuuacc cuguacaaaa acgaucuuau ccugaucaag     3120

gacacggaga caaauuguca gcaguucuuc cgcuuuugga gccggaccgg gaaagauaac     3180

cccaaguccu ucgaaaagca uaaaauugag cucaagccuu augaaaaagc caaguuugag     3240

aaaggagagg agcuggaggu gcucggcaag guucccccuu cuagcaauca auuccagaag     3300

aacaugcaga ucgaaaaucu guccauuuac aaagugaaga ccgacguccu ugggaacaaa     3360

cacuuuauca agaaagaggg cgaugaaccc aagcugaagu ucaaaaagug a              3411


<210>  3999
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  3999
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

aagaaagaua ccggagagau uauccacaca aauuccagac uguucucugc ugccacggcg      120

gacagcaaca uugaacggcg cagacaucgg ggcgggaagc gccucaccag acggaaaaag      180

caccgcucca ucagacugca ugaucuuuuu gaggacucug gacugcucac agauuucagc      240

aaagugucca ucaaucugaa ccccuaccag cugcgggugc aaggccucga caaucagcug      300

accaacgaag aacuuuuuau ugcucugaag aauaucguca agcgcagagg cauuucuuau      360

cucgaugacg ccagcgaaga ugggggaaca guguccagcg acuacggcaa agccguugag      420

gagaaccgga agcugcuugc ugaacagacc ccugggcaga uccaacugga ucgcuucgag      480

aaauauggac aggugagagg cgacuuuaca gucguggaaa auggggagaa gcggcgccuc      540

auuaacguuu ucaccacguc ugccuacucc aaagaggcug aaagaauccu gcggaagcag      600

caggaguuua auaagaaaau uaccgaugag uucaucgaga acuaucugac aauccucacc      660

ggaaagagaa aauacuauca cggcccgggc aaugaaaaga gccgcacaga cuacgggcgg      720

uacaccacga aaaaggaccc cgagggaaaa uauauuacac uggauaacau cuuuggcauu      780

cuuaucggga agugcaccuu cuacccugac gaguauagag ccucuaaggc guccuacaca      840

gcccaagaau uuaaucugcu caacgaucug aacaaucuua ccgugcccac cgagacgaaa      900

aagcugagcg aggaacagaa aaagacaauu aucaaauaug cuaagaccgc caagacacuc      960

ggagcuucua cccugcugaa auacaucgcc aagcucauug gcgcguccgu cgaccagauc     1020

cauggguauc gcauugaucc uaacaaaaag cccgaaaugc acacauucga gaccuaccgg     1080

aaaaugcaga gccuggagac gaucuccgug gaagagauuc cuagaaaggu gcuugacgaa     1140

cuggcccaca uccucacacu gaauaccgag cgcgaaggaa ucgaggaggc uauuaacgcc     1200

acccuuaagg auacauuuuc ucaagaccag guccuggaac ucguucaguu ccggaaaaau     1260

aacagcuccc uguucagcaa gggcuggcau aauuuuucuc ugaaacucau gauggagcug     1320

aucccggaac uuuacgagac cuccgaggaa caaaugacaa uucugacgag acucggcaag     1380

cagaaaagca aggagaccuc uaagcgcaca aaauauaucg augaaaagga gcugaccgaa     1440

gagauuuaca accccguggu ggcuaaaagc guccggcagg ccaucaagau uaucaaugag     1500

gccaccaaaa aguaugggau cuucgacaac auugugaucg aaauggcuag agagaauaac     1560

gaagaggaug cgaaaaaggg auacaaugaa cgccagaagg ccaaccugga cgagaaaaag     1620

uccgcuaugg agaaagccgc cuuucaauau aauggcaaga aagaacuucc ugacaacguu     1680

uuccacgggc auaaggagcu cgcuacaaag auucggcugu ggcaccagca gggagaaaaa     1740

ugucuguaca cgggcaagaa cauccccauu ucugaucuca uccagaauca auauaaauac     1800

gagaucgacc acauucugcc ucuuagccug uccuuugaug acagccucuc uaacaaggug     1860

cugguccugg ccaccgcgaa ucaggaaaaa gggcagagaa cacccuucca ggcucuugau     1920

uccauggacg augccuggag cuaccgggag uuuaagucuu augugaagga ccucaaacug     1980

cuguccaaca agaaaaagga uuaccuccug accgaggaag acaucagcaa aauugagguu     2040

aagcaaaagu ucaucgaacg caaucuugug gauacaagau auuccucucg ggucgugcug     2100

aacgcccucc aggacuuuua caaagagcau caguucgaua ccacgauuag cgucgugaga     2160

ggccaguuua caucccaacu gcgccggaag uggggacugg agaaaucuag agaaaccuau     2220

caccaucacg cuguugacgc ccuuaucauc gcugccagcu cccagcuccg ccuguggaag     2280

aaacagaaua acccgcugau uagcuacaag gagggccaau ucguggacca ggaaacaggg     2340

gagaucauuu cucucuccga ugacgaauau aaagagcugg ucuuuaaggc gccuuacgau     2400

cauuucgugg acacccuuaa gagcaaaaag uucgaggauu cuauccuguu uuccuaccag     2460

guugacagca aauauaauag aaagauuucu gaugccacca ucuacgcuac acggaaagcc     2520

aagcucgaca aggagaaaaa ggaauauacg uacacccugg gaaaaaucaa ggauauuuau     2580

gcucugggca gcgaaaaacu uaagacaggg uucuacaagu uucuccaccu guauaaaaag     2640

gacaaauccc aguuccugau guaccaaaag gaucgcaaaa ccugggacga ggugaucgaa     2700

aagauucucg agcaguacag acccuuuaag gagaaagaua agaacggaaa agaagucgac     2760

uucaauccuu uugagaagua ucggaucgaa aacggcccca uucacaaaua cagccgcaag     2820

gggaauggcc ccgugaucaa gucucugaaa uauuacgaca agaacauucc ugaguccuuc     2880

aucaauauca caccgagcaa cuccaaaaac aaggugauuc uuagacagcu gaaucccugg     2940

cggaccgaug uuuauuuuaa ccaucagacg gaaaaauacg agcuccuggg acugaaguau     3000

gcccaccuuc aauucgagaa aaagacaggc aauuacucua ucaccauuga caaguacaac     3060

gaaaucaaag agaaggaagg ggucagcgag caguccgagu uuaaauucac ccugcauaag     3120

aaugaucucc ugcucauuaa agacacagaa accaaggagc agcagcuguu ucgcuuccuu     3180

ucuagaacaa agccuaacgu gaaacacuau guggaacuga agcccuacag ccgggaugag     3240

uucgagaaaa augaacgccu caucgagauc cugggauccg ucgacaaguc uggcagauuu     3300

gugaaagggc ugggaaagag caaccuuucc auuuauaagg uucggacgga ugugcugggc     3360

aaucaacaca ucauuaaaaa ggaaggggag gaaccuaaac ucaaguucug a              3411


<210>  4000
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4000
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaggaua ccggaaaaau uauccacgcu uccucuagac uguuccccgc cgcgacagcu      120

gacaauaacg uggaacggcg cagcaauaga cagggccggc gccucaacag acggaagaaa      180

caucgcuccg ugagacugca agaucuuuuu gagggguacg gacugcucac ggacuucucu      240

aaggucagca ugaaucugaa cccuuaucag cugcgggugc agggcaugga aaaucagcuc      300

accaacgagg aacuguuugu ugcccuuaaa aauauuguga agcgcagagg caucuccuac      360

cuggaugacg ccucugagga ugggggaaca gucagcuccg acuauggcaa ggcuguggag      420

gaaaaccgga aacuccuggc cgagaagacc ccggggcaaa uccagcuuga acgcuucgag      480

aaauacggac agcugagagg cgauuuuaca guugaggaaa auggggagaa gcaccggcuc      540

auuaacgugu ucagcaccuc ugcuuaucgc aaagaagccg agagaauccu gcggaagcag      600

caagaauuua auuccaagau uacagacgag uucaucgagg auuaccugau uauccucacc      660

ggaaaaagaa aguauuacca uggccccggc aacgaaaaga gccgcacgga cuacgggcgg      720

uuuagaaccg auggaacaac ccuggacaau auuuucggca uccuuaucgg gaagugcaca      780

uuuuauaccg aggaauaccg cgcgucuaaa gccuccuaua cggcucagga guucaaccug      840

cucaacgacc ugaauaaccu uacagucccu accgaaacaa agaaacugag cgaggagcag      900

aagaagcuca uuaucgaaua cgccaaaucu gcuaagaccc ugggagccuc cacccugcuc      960

aaauauauug cgaagaugau cgaugccagc guggaccaga uucggggcua cagaguggau     1020

gucaauaaca aacccgagau gcacacauuu gaaguuuauc gcaagaugca aucccuggag     1080

acgaucaagg uggaggagcu uccucggaaa gugcuggacg agcucgcuca uauccugacc     1140

cuuaauacag aaagagaggg gauugaagag gccaucaacu cuaagcugaa agauauuuuc     1200

aaucgcgacc agguccucga gcuggugcag uuccggaaga acaauagcuc ccuguuuagc     1260

aaaggauggc acaacuucuc uaucaagcuc augauggaac ugauucccga gcuuuacgaa     1320

accuccgagg aacaaaugac aauccugacc agacucggca agcagcgcag caaagagacg     1380

ucuaagcgga caaaauacau cgaugagaag gaacugaccg aggaaauuua uaauccuguu     1440

guggcuaaaa gcgucagaca ggccaucaag auuaucaacg aggccaccaa gaaauacggc     1500

auuuuugaca auaucgugau ugaaauggcu cgggagaaca augaggaaga ugcgaagaaa     1560

gacuauauca agcgccagaa agccaaccaa gaugagaaga acgcugccau ggaaaaagcc     1620

gcuuuccagu acaaugggaa gaaggagcug ccggacaaca ucuuucacgg acauaaagaa     1680

cuuacaacca agauuagacu cuggcaccag cagggcgaga aaugucugua uacagggaag     1740

aauaucccca uuuccgaccu gauccauaac caauacaaau augagauuga ucacauccuc     1800

ccucugucuc uuagcuucga cgauucccug agcaauaagg uucucgugcu ggccacggcg     1860

aaccaggaaa agggacagcg gacccccuuu caggcucugg acucuaugga ugacgccugg     1920

uccuacagag aguucaaaag cuacgucaag gauucuaaac uucucuccaa uaagaaaaag     1980

gacuaucugc ugacagaaga ggauaucagc aagauugagg ugaaacaaaa guuuaucgaa     2040

cgcaaccucg ucgacacccg guacuccucu agagugguuc ugaaugcccu ucaggauuuc     2100

uauaaaagcc accagcugga cacaaccauu uccguggucc gcggccaguu uacgucucaa     2160

cucagacgga agugggggau cgagaaaagc cgcgaaaccu accaucacca ugcuguggau     2220

gcccugauua ucgcugccuc cagccagcug agacuuugga agaagcacuc uaacccucuc     2280

aucgcguaua aagagggcca guucguugac uccgaaacag gagagauugu gagccugucu     2340

gacgaggaau acaaggagcu ggucuuuaaa gcccccuaug aucauuucgu ggacacccuc     2400

cgguccaaga aauucgaaga uagcauccug uuuucuuacc aaguggacag caaguacaau     2460

cgcaaaauuu ccgaugcuac aaucuaugcc accagaaagg cuaaacuuga caaagagaag     2520

aaagaauaca cguauacacu gggcaagauu aaagauaucu acgcccucgg gaccaagaca     2580

ccgagcaaga ccggauucua uaaauuucug gaccuguaca agaccgauaa aucucaguuc     2640

cuuauguauc agaaggaccg gaaaacaugg gaugagguua ucgagaagau uaucgaacag     2700

uaccgcccuu uuaaggagua cgacaaaaac ggcaaggaag ucgauuucaa ucccuuugag     2760

aaauauagaa uugggaacgg accuauccgg aaguacucca aaaagggcaa ugggcccgaa     2820

auuaagagcc ucaaauauua cgacauccug cugggcaagc acaaaaacau uacgcccgac     2880

ggaucccgca acaccguggc gcuccugucu cuuaauccuu ggagaacaga uguguauuac     2940

aacagcgaga ccaagaaaua ugaguuccug ggccucaagu acgccgaccu gugcuuugaa     3000

gaagggggag cuuacggcau cuccgaaguc aaguauaaaa agauccggga gaaagagggg     3060

auuggaaaga acucugaguu caaauuuaca cuguacaaga augaucuuau ccugauuaaa     3120

gacaccgaaa cgaauuguca acaguucuuu agauucugga gccgcacagg caaggauaac     3180

ccgaaguccu ucgaaaaaca caagaucgag cucaaacccu augaaaaggc caaauuugag     3240

aagggggagg aacugaaggu gcucggcaaa guuccucccu cuagcaauca guuccagaag     3300

aacaugcaaa uugagaaucu guccaucuac aaagugaaga ccgacauccu uggaaacaaa     3360

cauuuuauua agaaggaagg cgaugagccu aaacugaagu ucaaaaagug a              3411


<210>  4001
<211>  3420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4001
augagcgacc uggugcucgg ccuggauauc gggauuggau ccgucggcgu ugggauccuu       60

aacaagguga ccggagagau uauccacaaa aauucuagaa uuuuccccgc cgcucaggcc      120

gaaaacaauc uggagcggcg cacaaacaga caaggccggc gccucacgag acggaagaaa      180

caucgcagag ugcggcugaa ucaccuuuuu gaagagagcg ggcugaucac cgacuucaca      240

aaggucucca ucaaccucaa uccuuaccag cugcgcguga aaggacugac cgcggaacuc      300

ucuaacgagg agcuguuuau ugcucuuaag aauaugguua agcauagagg caucagcuau      360

cuggaugacg ccuccgauga cggcaacucu agcguggggg auuacgccca gauugucaaa      420

gaaaauucca agcagcucga gacaaaaacc ccgggacaaa uccagcugga acgguaucag      480

aaguacggcc agcuucgcgg ggacuucaca guggaggagg auggaagaaa acaccggcug      540

auuaacguuu uucccaccag cgcuuaucau gccgaagcuc uccgcauccu gcaaacgcag      600

caggaguuca auccucagau uaccgacgaa uuuaucaacu cuuaccugga gauccucaca      660

ggcaagagaa aguauuacca cgggcccgga aaugaaaaau cccggaccga uuacggcaag      720

uauacaacca aaaaggacgc ccaaggccag uacauuacgc ugaacaacau cuucgggauu      780

cuuaucggaa aaugcacauu uuauccugag gaguacagag cggccaaggc uagcuauacc      840

gcccaggaau uuaaucugcu caacgaucug aauaaccuua cagugcccac cgagaccaaa      900

aagcugucug aggaacaaaa guaccagauu aucacauaug ucaaaaauga aaaggcuaug      960

ggcccugcca aacucuuuaa guacaucgcg aaacugcugu ccugugacgu ggccgacauu     1020

aagggguacc gcaucgauaa gagcgacaaa gcugagauuc acacguucga ggccuaucgg     1080

aagaugaaaa cccucgaaac acuggaugug aagaaaaugg cuagagagga acuugacaag     1140

cuggccuacg uccucacccu gaacacagag cgcgaaggaa uccaggaggc ccuugaucau     1200

gaguuugcug acggcaccuu cucucaggaa caaguugaug agcuggugca guuccggaag     1260

gcuaauucca gcauuuuugg gaaaggaugg cacuccuucu cugugaagcu caugauggaa     1320

cugaucccgg agcuguaugc cacgagcgag gaacagauga caauccucac cagacugggc     1380

aaacagaaga ccacauccag cucuaacaaa accaaguaca uugacgagaa gcaacuuaca     1440

gaagagaucu auaaucccgu cguggcuaaa uccguucgcc aggccauuaa gaucgugaac     1500

gccgcuauua aaaaguacgg cgauuuugac aauaucguca uugaaauggc ccgggagacg     1560

aacgaggaug acgagaaaaa ggcgauccag aaaauccaga aggcuaauaa ggccgaaaaa     1620

gacgccgcua ugagaaaggc cgcuaaccaa uauaauggga aagccgaacu gccucauagc     1680

guguuccacg gacacaagca gcucgcgacc aaaauucgcc uguggcauca gcagggcgag     1740

cggugccugu acacagggaa gaccaucucu auucacgauc uuaucaacaa ccccaaucaa     1800

uuugaaauug accauauccu cccucugagc aucacauucg augacucccu ggccaacaag     1860

guucucgugu acgcuaccgc caaucaggag aaaggacaga gaacgcccua ucaagcucug     1920

gauucuaugg acgaugccug gagcuuucgc gagcuuaagg cguucguccg ggaauccaaa     1980

gcccugagca acaagaaaaa ggaguaccuc cugaccgaag aggacauuuc uaaguuugau     2040

gugcggaaaa aguucaucga aagaaaucug gucgacacac gcuaugcuuc cagagugguu     2100

cuuaacgccc uccaggagca cuuucggacc cauaaaauug auacaaaggu gagcgucgug     2160

agaggccagu ucaccucuca gcugcgccgg cacuggggga ucgagaaaac gagagacaca     2220

uaccaccauc acgcuguuga ugcccugauu aucgccgcuu ccagccaacu caaucugugg     2280

aagaagcaga aaaacacccu uguguccuau ucugaagacc agcugcucga caucgagaca     2340

ggcgaacuga uuagcgauga cgaguacaag gaguccgucu uuaaagcgcc guaucagcau     2400

uucguggaua cccugaaguc uaaagaguuc gaagacagca uccuuuucuc cuaccaagug     2460

gauagcaagu ucaaucgcaa aauuucugac gccaccaucu acgcuacaag aaaggccaag     2520

cucgauaaag aaaagaaaga guauacguac acccugggaa agauuaaaga caucuaugcc     2580

cugggcacaa agaccccuuc caagacaggg uuuuacaaau uccucgaucu guauaagaaa     2640

gacaagagcc aguuucuuau guaccagaaa gaucggcgca ccugggacga aguuaucgag     2700

aagauucugg agcaguauag acccuucaag gaaaaagaua agaacggaaa agaggucgac     2760

uuuaauccuu ucgaaaagua ccggaucgag aacggcccca uucgcaaaua cucuagaaag     2820

gggaauggac ccgaaaucaa gucccucaaa uauuacgaca accugcuggg ccgguuugug     2880

gauauuacgc cuagcgaguc uaagaauccg guggcgcuuc ucagccugaa ccccuggcgc     2940

acagacgucu auuacaacac cgagaccaga aaauaugagu uccuggggcu caaguacgcc     3000

gaucuguguu uugaaaaagg cggauccuau ggcaucagca aggugaagua caauaaaauu     3060

cgggaaaagg aggggaucgg caaaaacucu gaguucaagu uuacacuuua caaaaaugac     3120

cugauccuca uuaaggauac cgaaacaaac cgccaacaga ucuucagauu cuggucccgg     3180

acgggaaaag acaauccuaa gagcuuugag aagcacaaac uggaacugaa gcccuaugag     3240

aaaaccagau ucgaaaaggg ggaggagcuu aaaguucugg gaaaggugcc ucccuccucu     3300

aaccgccucc agaagaauau gcaaauugaa aaccugagca ucuacaaagu ccggacagau     3360

gugcucggca aucagcacau uaucaagaac gagggggaca aaccgaagcu ggauuuuuga     3420


<210>  4002
<211>  3420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4002
augaacuacu auaauggcuu caucauuacc gagaacgcca uggcuagcaa gcacgaaaau       60

cugggggccc ucacauuugg acuggacauc ggcauugcgu ccguggggug ggcuguccuu      120

ggagagaaca gaaucauuga ucugggcguu cggugcuucg acaaagccga aacggccaag      180

gagggggaau cucucaaucu ggcucgcaga accgcccggc uucugcgcag acggcuccgc      240

agacgggcuu ggcgccugac aaaacuggcc agacuccuga agcgggaggg acuuaucgcg      300

gauaccaacg uguuucagaa acccuucaau cugaaggacg ccaacgagaa gggccucagc      360

uuuuggcagc ugcgcgugga ugcucuugac agaaaacugu ccgccgaaga gugggcucgg      420

gucaucuacc aucucuguaa gcaccgcggc uuccauugga uuucuagagc cgaagagaaa      480

gcggccgagg gggauagcaa guccgaagga ggcaaaguga agcaggggcu ggcuggaaca      540

aagaaacuga ugcaggagaa gaauuaucgg accgccgcug aaaugguucu cacagaguuu      600

ccugaagccc agagaaacaa acaaggcgac uacucuaagg cccugagccg cauccuucug      660

ggggaugaga ugacccagcu uuucaaacac cagcgggagc ugggaaaucc gcaugcuacg      720

gaaaagcucg aggcggccau ucugggcaac ggcgaccaca aauccgggcu cuuuugggcu      780

cagaaucccg cccuggccgg aaacgaucug cucaagaugc ugggccacug caccuucgaa      840

aaggcugagu auagagugcc uaaagccagc uuuacagcgg aacgccaugu cuggcuuacc      900

cggcugaaua accucagaau cgugguugac gggacaaccc gcccccugaa cgaggcugag      960

cggcaccuug cccugcucau gccuuaccaa caggccggag aucugacgua uaagcagcug     1020

agaacagcuc ucaccaaagc cggcuaccug cccgaaucuu uccgcuacac agggcuugcu     1080

uaugccuccg gacagaaggc ggacgagaaa gccaaggacc cugaaagcgc uccgcuggug     1140

aagcuccccg ccuggcauga gcugcggaaa acccuggaaa agaaagagcu ugcuucugag     1200

uggcaaaaga uuuccagcga ugcccucgac ggcaaaccuc agcugcugga ucagaucgcg     1260

uggguccucu cuguguacaa ggacgauacc gaaguggagg ccgaacugug uaaacuuaau     1320

cugcccaacg cugagaagau gauugacgcc cuccuggaua ucagauuuga caaguucucc     1380

aaucugagcc uuaaagcucu ccgccaaauc gucccucuga uggagcaggg ccagcgguau     1440

gaugaagccg uugccaacau ucccgaguac gggcaccacu cccagcugca ugaaguggga     1500

gcuggcgagc acaaguaucu cccgccuuuc uacaaagaaa gagacaagac agggcggaug     1560

guguuuaaug augacaugga caucccccgc aacccugucg ugcugagagc gcuuaaucaa     1620

gcccggaagg uugugaacgc ucugauuaaa gaguauggau cuccccauga gguccacauc     1680

gaaauggcca gagaucucag ccgcccccug gacgagcgga gagaugugga aaagcugcag     1740

aaagaguucc gcgacagaaa ugaaaaggau aaagcccuuu uugcucagga cuucaacauu     1800

guuggcgccg ugaaggggaa agaguuugag aaguaccagc ucuaccggga acaacagggc     1860

aagugcgcgu auuccaucga gccuauggau auccaucgcc uguucgaaca gggauacguc     1920

gagauugacc acgcucugcc guauagcaga ucuuuugaug acuccaaaaa uaaccgggug     1980

cucguccugg ccaaggaaaa ucgcgauaaa ggcaacagaa cgcccuucga guaccuuggg     2040

ggaaaggaca auagcgagcg guggcgccag uuuguggccu ucguugaagg caacaaaucu     2100

uauagacugg cuaagcggag ccgccuccug agaaaggauu uugggaaaga cgaggccaag     2160

ggauuccggg aaagaaaccu gaaugacacc cgcuacaucu gucgguuuuu caaaaacuau     2220

guggagcaau accuucagcu ccacgaggau uccgacgcga agagaugcgu cgugcugucu     2280

ggccagauga ccagcuuccu gcgcgcucgg ugggggcucg uuaaagugag aggcgauucc     2340

gaccgccauc acgcccugga uggagccauu gucgcugccu guagccauuc uauggugaag     2400

cggcuuuccg acuacagcag acgcaaggaa cuggaacagg ugcgggcugg cuuuguugau     2460

gucgacacag gggagaucgu gaauccugcc auguuccaac aggugcagga gcacuuuccc     2520

aacccuuggc cccacuucag agaugaaguc aaacuccggc ugaaugugga cgauccggcg     2580

cugcuucauu cugagaugaa ccgcuuugga acauauuccc cugaggcccu gaaggcucuc     2640

agaccccugu ucauuagccg ggccccucaa cgcagaaauu ccggcgcugc ccacaaagac     2700

accaucuacg cccagcccga acggcucguu gcucagggag gagugacgca gaaggucccu     2760

cugacaucuc uuacccugaa agaucucgac aagcugaucg acccccauag aaacgagaag     2820

cuguauagcg cgauucgcau ccggcuugaa caacacggcg ggaaaggcga uaaggccuuu     2880

ccggcuucca auccucugag aaaacccgac ucugagggaa accccaccgg cccuauugug     2940

cgcgggguua caauggugau cgauaagcuc agcgggauuc cgauccgggg cgggauugcc     3000

aaaaaugacu ccauggucag aguggauguu uucaccaagg ccggaaaauu ucaucuggug     3060

cccgucuacg ugcaccacgc uguggccaag guccucccua accgcgcggu uguggcuuau     3120

aaggacgaaa cagaguggac gcugaucgau gacggcuucg auuuuugcuu cagcauguac     3180

cccaaugacc uucugcggau caaacucaga aacgccucuc aucuggggua uuacgccucc     3240

uguaaucgca gcaccggcgc uauuaaccug uggucucacg auagaaacca acagguggga     3300

aaggacggca ugaucgaguc cauugggguc aaaacagccc uuaaugugga aaagcugaac     3360

aucgacguuc ucggaaaucg guacccugcg cccccugaga aacgcagaga ucuggcuuga     3420


<210>  4003
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4003
augaagaaca ugaccaaagu gaaugucggc cuggacguug ggaucagcuc cgccggaugg       60

ucugugauua acgcugagac aaaucagauc cucgaaagcg gcgugagacu guucuccggg      120

ucugaugccg acgagaacgu caaucggcgc aaccaaagac agggacggcg ccuuuuuaga      180

cggcgcaagc ugcggauuca ggaagcgaaa aaguaccucg agaauguguu ccagcugaaa      240

gauaucgaac ccaaggacga guggaacccu uaugagauuc ggguucgcgc ucuuaaugaa      300

aaggugagca aagaggaacu guccaucgcc cuccugaaca ucgucaagca cagaggcauu      360

ucuuacgagc uggaggaugu gacggacgaa gaggggacca auacacagag cuuucaaucc      420

ggacucgauu auaaccggcg ccugcuuaaa accaagacag uuggcgaaau ccaguacgac      480

agacugcaga aauauggcca agugcggggg cagauucaga ccgaggaugg acuccugauc      540

aauauuuucc cgacaaagaa cuacgucgaa gagguggagc gcauccuuga aacccagaag      600

aaauaucauc ccgagauuga cgauguguuu aucgaaaaga ucauuagcuu cgucacgaga      660

aaacgggagu acuuuguugg cccugggaau gaaaagucua gaaccgacua cggaaucuau      720

cgcgagaacg gcgagacacu ggauaaccuc uucgaaauuc ugaucgggaa agacaaguuu      780

uaccccgagg aauaucgggc cgcuggaucc agcuacaccg cccaaauuuu caaucugcuc      840

aacgaccuga auaaccuuag aaucccuaca accgaggauc accaucugac gaaagcucag      900

aaggaagaga ucauuacaga gcucaagaau accucucgcc uguccggcgg caugcuuaaa      960

cugaucagca aggugacaaa agcccccaag gaagagauuu cuggguaucg gaaagacgaa     1020

aaggauaagc cugaccucca cucccuggcg uacuauagaa aacagaagaa caugcuggag     1080

aaagagggaa uccaaauugc cgauuuuagc gaggaacucc uggacgaacu ugcuaccaug     1140

augacccuga auacagagcc gggcgaaauc cgcaagcagc ucaacaaaau cacgaaucag     1200

uacgauauuc ugacccccga ggugaucgag guccuuguga agaaccauac agccuucguu     1260

gcuaccucca aucacaagug gcaccgguuu ucucugaaac ucaugaagga guucauuccu     1320

gaacugcugg aaacaagcaa agagcagucc accauccucu gcgaaagagg guucauucaa     1380

aaccgcgcca gcuaccugga cgagaaucau cacuuuaacu augcggagcu ucgggaaaau     1440

cuguacaacc ccguggucag aaagucuacg ugugagaugc ucaaaguguu cacagccauc     1500

caggaaaagu auccuaacau ucagaauguu aucguggagc ugccccgcga aaccaacgag     1560

gaugagcaga agcggaaaau caagaaaauu aaugacaaca augcuaagag aaagaaggaa     1620

auccugaaag aggcccaagc ucaguccgga cuuagcgaag aggcccucga ggaaaagcug     1680

cugaagacca aaggccucua ccugaaacuu augcuguggu augagcagga agggaaaugc     1740

gucuacucug gacaggagau uccgcucagc gaucugcugc aaaacaaggg cggcuaugaa     1800

guggaccaua ucauuccuau cuccaucucu uuugacgauc ggcuuuguaa uaaaguccuc     1860

gugcgccagc agcugaacag ccagaagucc aaucaaacac ccuucgagua caugaccaca     1920

cacacggacg ggcaccugag cuuucaugag cucaaggaac ugguuggaaa gaacaagaga     1980

uucucuaaag agaagcggga acuucugcuc gccgagauuu acuccaauga uaucgaaacc     2040

agaaaacgcu uuauuaaccg gaaucuggug gacacaagcu auaccgcuag aaucguccug     2100

aacgcgcuuc agcaguacaa gaaggagaau caacucucua ugaacguguc cguugugcgc     2160

ggcgccauga caaacauucu gcggaaaaag gucaaacugg auaagaccag agagacguuc     2220

caccaucacg cuaucgacgc cagccucaug gccgugaucc cuaauauuga auuuuucaaa     2280

gagcgcaagg aauggcugcc caaguccgcu aacgaggaag ugguugaggu cgaggugucu     2340

gaagaggaaa ccaugcccga gaaagaguuu aaggugaaaa ugagacuucc ugagagcuuc     2400

auccaggaug uccagauucu gcagagaaau uccuuugcca aggaagaagu gucugggccg     2460

caaacacgcg agagaaaccu caaaaucaag uucagccauc agguugacca gaaaaugaau     2520

cggaaggugg cggaugacac cauuuaugga acacgccagg cucaacuggg caaggauaaa     2580

aaggaagaga ccuacgucau cgggaaaauc aaggacauuu auaacuacga ugaguaugug     2640

agauucaaaa agcgguacga caagaauaaa gaauccuuuc ugauguacca ccacgacccc     2700

cagacguucg agaaacuuga agagauccuc gcccaguauc cugaauacau ugaggaugag     2760

aaaggcaaga aaaagaaggu uagccccuuu gcccuguauc gcgaagagaa cggaccugug     2820

acaaaauacg cuaagaaaaa uaacggcccc gucaucaagc agcugaaaua uuacgacucu     2880

aagcucaaua gaaagauuga uaucucccau aaauauagca ccaagaaccg gcaagugguu     2940

cugcagucuc uuaaaccgua cagaacagac guguaccgca aucaggauac cggggaauau     3000

acccuggucg gaauugacca aaacgauggc aaguucguga augggcacua cggaaucccu     3060

aaaucccggu augaggaaau caaggccaac aacaagcuca gcgagcauga cgaguuugug     3120

uucucucugu acaaaaauag cauggucaag guuauugaug aaaacggcga guccaucgug     3180

gugagauauc ugagccgcuc uaugcccaau cggaaaaaca augcugaaau uaagccuguc     3240

gagaaauacc aguuugagga cggggaagug guucccgugu auggcaagug cagaaaacag     3300

augcagacag ucuucgugaa gaagggaugg acgcuucuga aaggcaacac cgauauccuc     3360

gggaauacau acuuuguuga gaaggaaucc gagaacccua aaaagaugau ugacgauaaa     3420

uga                                                                   3423


<210>  4004
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4004
augagagaga acggcagcga cgaacggcgc agaaauaugg augagaagau ggacuaccgg       60

aucgggcugg auauuggaau cgccuccgug ggcugggcug uccuccagaa caauucugac      120

gaugaacccg uucgcauugu ggaccugggg gugagaaucu ucgauaccgc cgagauuccu      180

aaaacaggag aaagccuugc gggcccgcgg cgcgcugcca gaacgacccg gcgcagacug      240

cggcgcagaa agcaccggcu cgaccgcauc aaauggcugu uugagaacca agggcuuauc      300

aauauugaug acuuccugaa gagauauaac auggccggac uccccgaugu cuaccagcug      360

cgguaugagg cucuggacag aaaacucaca gaugaagaac uggcccaggu gcuucugcau      420

aucgcuaagc accgcggcuu ucgguccacc agaaaggccg aaacagcggc caaagagaau      480

ggcgcuguuc ucaaggccac cgacgagaac cagaaacgca ugcaagaaaa gggguaccgg      540

acagugggag agaugauuua ucuggacgag gcuuucagaa ccggcugcuc uuggagcgag      600

aaaggguaca uccuuacgcc ucgcaauaag gccgaaaacu aucagcauac caugcugcgg      660

gcgaugcucg ucgaggaggu gaaggaaauc uuuuccucuc agagacgccu gggaaaugag      720

aaagccacag aagaacugga agagaaguac cucgagauua ugaccagcca gcgguccuuc      780

gaucugggcc ccgggaugca accugacgga aaacccagcc cuuacgcuau ggaaggcuuu      840

ucugauagag uuggcaagug uacauuccuu ggggaccagg gagagcugcg cggcgccaaa      900

gggaccuaua cggcugaaua cuuuguggcc cuccagaaga uuaaccacac aaaacugguc      960

aaucaggaug gagagacccg gaacuucaca gaagaggaga gacgggcccu uacccugcuc     1020

cuguuuaccc aaaaggaagu gaaguaugcu gcggugcgca aaaagcuggg ccucccggag     1080

gacauccugu ucuacaaucu uaacuauaaa aaggccgcua caaaagaaga acagcagaag     1140

gagaaccaaa auacggaaaa ggccaaauuu aucgggaugc ccuacuauca ugauuacaag     1200

aaaugccugg aggaaagagu caaguaccuc accgagaacg aaguucggga ccuguucgau     1260

gagauuggaa ugauccuuac auguuauaaa aaugacgauu ccagaaccga gcgccuggcc     1320

aagcucggcc uggugccuau ugaaauggag ggccugcucg cuuacacacc caccaaguuc     1380

cagcaccuga gcaugaaagc caugcggaac aucauuccuu uucuugaaaa ggggaugacg     1440

uaugacaaag cgugcgagga agcuggauac gacuucaagg ccgauucuaa aggcacaaag     1500

cagaagcugc ucaccgggga gaaugugaac cagaccauca augagaucac aaaccccguc     1560

gugaaaagau ccguuagcca aaccgugaag gucauuaaug ccaucauucg cacauaugga     1620

ucuccgcagg cuaucaacau ugaacuggcc agagagaugu ccaaaacguu ugaggagcgg     1680

cgcaagauca aaggcgacau ggaaaagaga cagaaaaaua acgaggaugu gaagaagcag     1740

auccaagagc uggggaaacu uagcccuacc ggccaggaca uucucaagua ccggcugugg     1800

caggaacagc aaggaaucug uauguauucc ggcaaaacaa uuccccugga ggaacucuuc     1860

aagccugggu acgauaucga ccacauucug cccuacucua ucaccuuuga ugacagcuuc     1920

cgcaauaaag uucuugugac aucccaggag aacagacaga agggaaaucg gacccccuau     1980

gaguacaugg gcaacgauga acagcgcugg aacgaguuug aaacgagagu caagaccaca     2040

auucgggacu auaaaaagca acagaaacug cucaagaaac auuucagcga ggaggagcgc     2100

ucugaguuua aggaaagaaa ucugaccgau acaaaguaca ucaccacggu gaucuauaac     2160

augauucggc agaaucugga gauggcuccu cuuaacagac cggagaaaaa gaaacaaguc     2220

cgcgccguga auggggcgau cacagccuac cuccggaaga gauggggacu gccccagaaa     2280

aaccgcgaaa ccgacacaca ccaugcuaug gaugccguug ugauugcuug cuguaccgac     2340

ggcaugaucc agaagauuuc ccgguauacc aaggucagag aacgcugcua cagcaaaggg     2400

acagaguucg uggacgccga gacgggcgaa aucuuucggc cugaggauua cucuagagcg     2460

gaaugggacg agaucuucgg aguucacauu cccaagccuu gggaaaccuu ucgcgccgag     2520

cuggaugugc ggaugggcga cgaucccaaa ggguuccucg acacacacag cgaugucgcu     2580

cuggagcuug acuauccgga auacaucuau gagaaucuga gaccuauuuu cgugucccgg     2640

augcccaacc auaaggugac cggagccgcu cacgccgaua caauccgcuc uccuagacau     2700

uuuaaagacg aaggcauugu ucucaccaag acggcccuga cagaucugaa acuugacaag     2760

gauggggaga ucgacggaua cuauaauccc cagagcgacc uccugcugua cgaggcucuc     2820

aagaaacaac ugcuucugua uggcaacgau gcgaagaaag ccuucgcuca ggacuuucac     2880

aagccuaaag ccgaugggac cgaaggcccc gucgugcgga aggugaagau ccagaaaaag     2940

cagaccaugg gagucuucgu ggacuccggc aaugggauug ccgagaacgg aggcaugguu     3000

cgcaucgaug uguuuagagu caaugggaaa uacuacuucg ugccgguuua uacagcugac     3060

guggucaaga aagugcuccc uaaccgggcc agcaccgcgc auaagcccua cggagaaugg     3120

aagguuaugg aggauaaaga cuuucuguuc ucucuguauu ccagagaucu uauucacauc     3180

aagagcaaaa aggacauucc caucaaaaug gugaauggcg ggauggaagg caucaaggag     3240

acauacgcuu auuacauugg agccgauauc ucugccgcua acauucaagg caucgcccac     3300

gacucccgcu auaaguuucg ggggcucggc auucagagcc uggacguccu ggagaaaugu     3360

cagaucgaug ugcucggaca uguguccguc guuagaucug aaaagcgcau gggguucagc     3420

uga                                                                   3423


<210>  4005
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4005
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

gagaaaaauu ccggaaaaau ugugcacgcu ucuagcagaa ucuuccccgc cgcgaccgcu      120

gauaacaaug uggaacggcg caagaacaga caggcccggc gccugaauag acggaaaaag      180

caucgcggcg ucagauuuca agaccucuuc gaggauuacg ggcugcuuac agacuuuucc      240

aaagugucua uuaaccugaa uccuuaucgg cuccgcguug auggacugaa ccagcagcug      300

acgaaugaag aacucuucau cgcccugaag aacaucguga agagacgggg cauuagcuac      360

cuugacgaug cuuccgaaga cggcgggacc gucucuagcg auuauggaaa agccguggag      420

gagaaucgca agcugcucgc ugaaaagaca ccgggccaga uccaacugga gagauuugaa      480

aaguacgggc agcuucgggg agacuucacc guuguggaga acggcgagaa augccgccug      540

auuaaugucu uuuccacaag cgccuauaag aaggaagcgg agagaauccu ccggaaacag      600

caggauuuca acaaucaaau uaccgacgaa uuuaucgagg auuaccugaa gauucugaca      660

gggaaaagaa aguauuacca cggacccggc aacgaaaaau cucgcaccga cuacggccgg      720

uauacgaccc auaaggacuc caaaggggag uacgugacac ucgauaacau cuucggagug      780

cugaucggca aguguaccuu uuauccuaau gaguacagag ccagcaaggc uucuuauaca      840

gcccaggagu ucaaccuucu gaaugaccuc aacaaucuga ccguccccac ggagacaaag      900

aaacuuuccg aggagcagaa aaagaccauu aucgaauacg cuaaaagcgc caagacacug      960

ggggcgucua cccuccugaa guauauugcc aaaaugaucg auaccuccgu ugaccagauu     1020

cgcggauacc ggguggaugu gaacaauaag ccugagaucc acacauuuga ggucuacaga     1080

aaaaugcaaa gccuggaaac gaucuccgug ggcgagcucu cucgcaacgu ucuggacgaa     1140

cuugcucaua uucugacccu caauacagag cgggaaggga ucgaggaggc cauuaacacc     1200

aagcugaaag auagcuucuc ccaggaccag gugcuugaac ugguccaauu cagaaagaau     1260

aacagcucuc ucuuuuccaa gggauggcac aauuucagcc ugaaacugau gauggagcuc     1320

auccccgaac uguaugagac aucugaggaa cagaugacca uucuuacgcg ccugggcaag     1380

cagaaaagca aggagacauc caaacggacc aaguacaucg augaaaagga gcucaccgaa     1440

gagaucuaua acccuguggu ugcuaaaucu gugagacagg ccauuaagau cauuaaugag     1500

gccacaaaga agcacggcau cuuugacaac auugucaucg aaauggcucg cgagaacaau     1560

gaagaggaug cgaaaaagga cuacauuaaa cggcaaaagg ccaaccagga cgaaaaguau     1620

gcugccaugg agaaagccgc uuuccaguac aaugggaaga aagagcugcc ggauaacauc     1680

uuucauggac acaaggaacu ggccaccaaa aucagacuuu ggcaucagca aggcgagaag     1740

ugccucuaua caggaaagag cauucccauc uccgaccuga uucacaauca guacaaauac     1800

gaaaucgauc acauucugcc ucucagccug ucuuucgacg auucccuuag caacaaggug     1860

cugguccucg cgacggcuaa ucaggagaaa ggacagcgga cccccuuuca agcccuggac     1920

ucuauggaug acgccugguc cuaucgcgag uucaagagcu acgugaaaga auccaagcug     1980

cuuggcaaca agaaaaagga guaucuccug acagaagagg auaucucuaa aaucgaaguu     2040

aagcagaaau uuauugagag aaaucuggug gacacccggu acagcuccag agucgugcuc     2100

aacgcucugc aggauuucua uaagaaucau aacuuugaca caaccaucuc uguugugcgc     2160

gggcaguuca cgagccaacu ucggagaaag uggggccugg agaaaucccg cgaaaccuac     2220

caccaucacg ccgucgaugc ucucauuauc gccgcgagcu cucagcugag acuguggaag     2280

aaacagaaua acccucuuau ugccuauaag gagggacaau uuguggacuc ccagacaggc     2340

gaaaucauca gccucaccga cgaugaguac aaagaacugg uguucaaggc ucccuacgac     2400

cauuucguug auacacuguc uuccaaaacc uuugaggaca gcauucucuu cucuuaucag     2460

gucgauagca aguuuaaucg gaagaucucc gacgccacga uuuacgcuac acgcaaagcc     2520

aagcuggaua aagagaagaa agaauauacc uacacacuug ggaagaucaa ggacauuuau     2580

agccugggaa ccaaaacccc gucuaagaca ggcuucuaca aauuucucga ucuguauaac     2640

aaggacaaau cccaguuccu gauguuucaa aaggauagaa agacguggga cgaggugauc     2700

gagaaaauca uggaacagua ccggccuuuc aaggaauacg auaaagcggg gaaacuugug     2760

gacuuuaacc ccuucgagaa auaucgccag gagaauggac cuauuagaaa guacagcaag     2820

aaaggcaacg ggcccgaaau caagucccuc aaauauuacg acauucugcu gggcaagcac     2880

aaaaauauca cccccgaggg aucucggaac acagucgccc uccugagccu uaauccuugg     2940

cgcaccgaug uguauuacaa cauggaaaca aagaaguaug aguuucuggg ccucaaauac     3000

gcugaccugc cguucgaaga agggggagcc uacggcauuu ccaccgagac guauaaugaa     3060

cugagagaga aggaagggau cgggaaaaac ucugaguuua aguucacacu uuacaaaaau     3120

gaucugauuc ucaucaagga caccgagacc aacugucagc aauucuuucg guucuggagc     3180

cgcacaggca aagauaaucc caaguccuuu gaaaagcaca aaaucgagcu gaagccuuau     3240

gaaaaagcua aguucgagaa aggggaggaa cucgagguuc ugggcaaggu gcccccuucu     3300

agcaaccagu uucagaagaa uaugcagauu gaaaaccuuu ccaucuacaa agucaagacc     3360

gacgugcugg gaaauaaaca uuucauuaag aaagagggcg auaagcccaa gcucaaauuu     3420

uga                                                                   3423


<210>  4006
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4006
augaacggcc uggugcucgg gcuggacauc ggaauugcca gcgucggcgu ugggauccuu       60

aagaaagaua uuggagagau cauucacacc aauuccagac uguucucugc ugccacagcg      120

gacagcaaca ucgaacggcg cggccauaga gggggaaagc ggcucacgcg ccggaaaaag      180

cacagaucca uccgccugca ugaucuuuuu gaggacuucg gccugcucac cgauuuuucu      240

aaagugagca uuaaucugaa ccccuaccag cugagagugc aaggccucga caaucagcug      300

acaaacgaag aacuuuucau cgcucugaag aauauuguca agcggcgcgg gaucuccuau      360

cucgaugacg ccucugaaga uggaggcacc gugagcuccg acuacgggaa agccguugag      420

gagaacagaa agcugcuugc ugaacagaca ccuggacaga uucaacugga ucgguuugag      480

aaauauggcc aggugcgcgg ggacuucaau gucguggaaa acggagagaa gagacggcuc      540

aucaauguuu uuaccacaag cgccuacucu aaagaggcug aacgcauucu gagaaagcag      600

caggaguuca acaagaaaau caccgaugaa uuuaucgagg acuaucugac gauucucacc      660

ggcaagcgga aauacuauca cggcccgggg aaugaaaagu ccagaacaga cuacggacgc      720

uacaccacca aaaaggaucc cgagggcaaa uauaucacac uggacaacau uuucgggauc      780

cuuauuggaa agugcacguu uuacccugau gaguaucggg ccagcaaggc gucuuacaca      840

gcccaagagu ucaaucugcu caacgaccug aacaaucuua ccgugcccac agagaccaaa      900

aagcuguccg aagaacagaa aaagaccauc aucaaauaug cuaagacagc caagacgcuc      960

ggcgcuagca cccugcugaa auacauugcc aagcucaucg gggcgucugu cgaucagauu     1020

cauggauaua gaaucgaccc uaacaaaaag cccgaaaugc acacauuuga gaccuaccgc     1080

aaaaugcagu cccuggagac aauuagcgug gaggagcuuc cucggaaggu gcuggaugaa     1140

cucgcccaca uccugacccu uaauacggag agagaaggca ucgaggaggc uauuaacgcc     1200

acacugaagg acaccuucuc ccaagaucag guccucgaac ugguucaguu ccgcaaaaau     1260

aacucuagcc uguuuuccaa gggcuggcau agcuucucuc ucaaacugau gauggagcuu     1320

aucccggaac uguacgagac cuccgaggaa caaaugacaa uucucacccg gcuggggaag     1380

cagaaaagca aggagacauc uaagagaacg aaauauaucg acgaaaagga gcugaccgaa     1440

gagauuuaca aucccguggu ggcuaaaagc guccgccagg ccaucaagau uaucaacgag     1500

gccaccaaaa aguauggaau cuuugauaau auugugaucg aaauggcucg ggagaacaau     1560

gaagaggacg cgaaaaagga auacauuaag agacagaaag ccaaccuuga cgagaagaau     1620

gcugccaugg agaaagccgc uuuccaauau aacggcaaga aagaacuccc ugauaauguu     1680

uuucacgggc auaaggagcu ggccacaaag auccgccugu ggcaccagca gggagaaaaa     1740

ugucucuaca caggcaagaa cauucccauc uccgaccuga uccagaacca auauaaauac     1800

gagauugauc acauccuucc ucugucucuc agcuucgacg auucccugag caauaaggug     1860

cugguccuug cgaccgcuaa ccaggaaaaa gggcagcgga cgcccuuuca ggcccucgac     1920

ucuauggaug acgccugguc cuacagagag uucaagagcu augugaagga uucuaaacug     1980

cugggcaaua agaaaaagga guaccuccug accgaagagg acauuuccaa aaucgaaguu     2040

aagcaaaagu uuauugagcg gaaccuugug gauacacgcu auagcuccag agucgugcug     2100

aaugcucucc aggacuucua caaagagcau caguuugaua ccacaaucuc ugucgugcgg     2160

ggacaguuca ccagccaacu gagacgcaag uggggccugg aaaaaucccg ggagacguau     2220

caccaucacg ccguugacgc ucuuaucauu gccgcgucua gccagcucag acuguggaag     2280

aaacagaaca auccgcugau cuccuacaca gaagggcaau uuguggacca ggucaccgga     2340

gagauuauca gccucucuga ugacgaauau aaggagcugg uguucaaagc cccuuacgau     2400

cauuucguug acacacuuaa guccaagaaa uuugaggaua gcauucuguu cucuuaccag     2460

guggacucca aguauaaccg caaaaucagc gaugcuacca ucuacgccac cagaaaggcu     2520

aaacucgaca aggaaaauaa ggaguauaca uacacgcugg gcaaaauuaa ggauaucuau     2580

gcccugggga ccaaaucucc cagcaagaca ggauuuuaca aauuccuuga ccucuauaac     2640

aaggauaagu cccaguuucu gauguuccaa aaagaccgga agaccuggga ugaagucauu     2700

gagaaaauca uugaacagua ccgcccuuuu aaggaguacg acgagaaugg caaagaagug     2760

gacuucaacc ccuuugagaa guauagaauc gaaaaugggc ccauucggaa guacagcaaa     2820

aagggcaacg gaccugagau caaaucucug aaguauuacg auaaucuccu gggcaaauuc     2880

guggacauca caccguccga aagcaagaac cccguugcgc uucugucccu caacccuugg     2940

cgcaccgaug ucuauuacaa uacggagaca ucuaaguaug aguuucuggg gcugaaauac     3000

gccgaccuuu gcuucgaaaa gggaggcgcu uacgggauua gcgaggugaa auauaacaag     3060

aucagagaaa aagagggaau uggcaaggag uccgaauuua aauucacccu guacaagaau     3120

gaucucaucc ugauuaagga caccgagaca aacugucagc agaucuuccg guuuuggucu     3180

cgcaccggga aagauaaucc caagagcuuc gaaaaacaca agaucgagcu caaaccuuau     3240

gaaaaggcca gauuugagaa gggcgaggaa cuggaggugc uuggaaaagu gcccccgucc     3300

ucuaaccaac ugcagaagaa uaugcagauu gaaaaccuca gcaucuacaa agucaagaca     3360

gacguucugg gcaauaaaca cuucauuaag aaggaggggg aggaaccuaa acugaaguuu     3420

uga                                                                   3423


<210>  4007
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4007
auggaggaaa acuuccagua cagaaucggc cuggacauug ggaucaccag cgugggaugg       60

gccguccucc aaaauaacuc ccaggaugag cccguucgga uuacagaccu gggcgugcgc      120

aucuuugaug uggcugaaaa uccuaagaac ggggacgccc uugcggcucc gagacgggau      180

gcccgcacga ccagacggcg ccugagacgg cgcagacacc ggcucgagcg cauuaaauuc      240

cugcuucagg aaaauggacu gaucgagaug gacucuuuua uggagagaua uuacaagggc      300

aaccuccccg augucuauca gcugcgguac gaagggcugg accgcaaacu caaggaugag      360

gaacuggccc aagugcuuau ccauauugcu aaacacagag gauuccggag cacaagaaag      420

gccgagacca agggcaaaga gggcggggcu guucugaagg ccacaaccga aaaucagaaa      480

aucaugcagg agaagggaua ucgcacagug ggcgaaaugc ucuaccugga cgaggcguuu      540

cauaccgaau gccuuuggaa cgagaaaggg uauguccuga cgccucggaa uagacccgau      600

gacuacaagc acaccauucu ccgcuccaug cugguggagg aaguucaugc caucuucgcu      660

gcccagcggg cucugggaaa cgagaaggcc acagaggaac ucgaaaaugc guacguggag      720

auuaugaccu cucaaagauc cuuugauaug ggcccugggc ugcagcccga cggaaaaccu      780

uccccguaug ccauggaggg cuucggcgac cgcgucggga aguguacauu ugaaaaagau      840

gaguaccggg cucccaaggc caccuauacg gcugaacuuu ucguggcccu gcagaaaauc      900

aaccacacaa agcucauuga cgaguuugga accggcagau ucuuuacaga agaggagcgc      960

aaaaccauca ucgaucugcu ucugucuggg aaggaacuca aguacggaac cauucggaaa     1020

aagcugaaua ucgacccuag ccugaaauuc aacucccuca auuauagcgc caagaaagag     1080

ggcgaaacag aggaggaaag agugcgcgau acggagaagg cuaaguucgc gucuauguuu     1140

uggaccuacg aauauuccaa augccugaag gaccggaacg aggaaaugac agucgggcag     1200

aaagccgauc uucuggacag aauuggagag auccucaccg cuuacaagaa cgaugacagc     1260

cggucuuccc gccuggagga ucuuggccug agcagagaag agauugacgg ccuccuggaa     1320

cugucucccg ccaaauacca acggguuucc cucaaggcca ugagaaagau gcagccuuau     1380

cuggaggacg ggcuuaucua cgauaaggcu ugugaagccg cgggauauga cuuccgcgcu     1440

cugaaugaug gcaacaagaa acaccuccug aagggggagg agaucaaugc cauugugaac     1500

gacaucacaa aucccguggu caaacggagc gugucccaga ccauuaaggu uaucaacgcc     1560

auuauccaaa aguacggauc uccgcaggcu gugaauaucg aacuggccag agagaugagc     1620

aaaaacuuuc aggaucgcac gaaucuugaa aaggagauga aaaagagaca gcaagaaaac     1680

gagcgggcua aacagcagau uaucgagcuc ggcaagcaga auccuacagg gcaagacauu     1740

cugaaauauc gccuguggaa cgaucagggc ggauacugcc ucuauuccgg caagaagauc     1800

ccccuggagg aacuuuucgg gggaggcuac gacauugauc auauccugcc uuacagcauu     1860

accuuugacg auucuuauag aaauaaaguc cucgugaccg cccaggaaaa ccggcagaag     1920

gggaaucgca cacccuacga guauuucgga gcggacgaga aacacuggga agauuacgag     1980

gccagaguuc ggcugcuggu gcgcgacuau aagaaacaac agaaacuucu caagaaaaac     2040

uuuaccgaag aggaaagaaa ggaguucaaa gagcggaacc ugaaugacac aaaguacauc     2100

acgcgcgucg uguauaacau gaucagacag aaucuggaac ucgagcccuu uaaccauccu     2160

gagaaaaaga agcaggucug ggcugugaau ggcgccguua ccuccuaccu gcggaaaaga     2220

ugggggcuua ugcaaaagga ucgcagcaca gaccggcacc acgcuaugga ugccgugguc     2280

auugcguguu gcaccgacgg caugauccau aaaauuucua gauacaugca gggacgcgaa     2340

cuggccuaua gccggaacuu caaguuuccg gaugaagaga caggcgagau ccucaauaga     2400

gacaacuuca cccgcgaaca gugggaugag aaauuugggg ugaagguucc ccugccuugg     2460

aauagcuucc gggacgaacu ggauauuaga cuucucaacg aggaccccaa gaauuuccug     2520

cugacgcacg cugaugugca acgcgaacuc gacuacccug gauggaugua uggcgaggag     2580

gaaucuccca ucgaggaagg gcgguacauc aacuauauua gaccgcuguu ugucagccgg     2640

augccuaauc auaaagugac cggauccgcc cacgaugcua caauccgcag cgccagagac     2700

uacgagaccc ggggcguggu uauuacaaag gucccccuua ccgaccugaa acucaacaag     2760

gauaaugaga ucgaagggua uuacgacaaa gauucugacc gccugcugua ccaggcccuu     2820

gugagacagc uccugcugca uggcaacgau ggaaagaagg cuuucgcgga ggacuuucac     2880

aaaccuaagg ccgauggcac ggaagggccc gugguccgga aagugaagau ugagaaaaag     2940

cagacauccg gaguuauggu gagaggcggg accggaaucg cugccaacgg cgaaaugguc     3000

cgcaucgacg uguuccggga gaaugggaaa uauuacuuug uuccugugua uacagccgau     3060

gucgugagaa agguucuccc caaccgcgcu gccaugaaug gcaagauugc gagcgacugg     3120

aaaaccaugg aggauaacaa uuucguguuu ucucuguacu cccgggaccu uauccacguc     3180

aagcgcaaaa aggcuauucc gaccauccug agcuccggag ccacauucga acaagaggaa     3240

uuuuaugccu acuucacggg cgcugacauu ucuaccguga gcaucgccgg gaucuccaac     3300

gauaauucuu uuaaauucag aggacucggc auucaggggc uggagaucuu ugaaaagugu     3360

cagguggaca uucugggaaa caucagcguc guucggcaug agaauagaca gggcuuccac     3420

uga                                                                   3423


<210>  4008
<211>  3426
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4008
auguacagca ucggccugga cauugggauc gccuccgugg gauauucugu cauugagacc       60

aacagcggca gaaucguuga acucggggcu cggauuuucg aggcccgcaa uuccgauaac      120

aauaaggaca gacgggauuu ucgcggaggc agacggcuga uccgcagacg gaaaacacgc      180

cuugacgaug cgaagagaua ccuggacgau cagggguuca aaaacgacaa gucucucauc      240

aaugugugcc ccuauagccu gcgggugaaa ggacuuacgg aggaacuguc caagucugaa      300

auucgcaagg ucauccucca cauucugaaa aagagaggcg ugagcuaccu guccagcgag      360

gaucucgaca ccucuuccgu ugauaacaaa gacagcuuua agcaagcugu gaccaaaaau      420

cugcggcagc uugaugacaa gacaccuggc cagauccagu augagcgccu gcaagaaucu      480

gggagagucc ggacaggaau uaaccagaau ggccaguacc agcucaacgu guucgacguu      540

gccuccuaug ccaaggagcu gaaacuuauc cuggaaaccc aacagcagua ccaucaggag      600

auugaugacc acuuucucga uuucuuuaca aaggacgggu acagagagga ugcuggacug      660

aucuaucgca aacggccgua cuaugacggc cccgggaaug ccgcucaaaa cagcucuuac      720

ggaagauggg ccaauuauca gguggauggc aagccugcgg aaaacaucuu cgaccagcug      780

auuggcaaag auuuuaccgg gaagauccgc gccuccaauc ucggacccag cgcucaaaaa      840

uucaaccugc uuaacgaccu gaauaaccuc cggauuacga ccagagagga agagaagcug      900

acauuugaag agaagcagga gauccuugaa guccuggaga augaucgccc uaaaaccuuc      960

ggcccccgga acauugccaa gaugcucaaa guggacaugg acgauaucaa gggguggaga     1020

cucaauuccu cugacaaacc ugaaauccau acaaugaagg aguacaacga uaugcgccug     1080

gugcuggaag cuggaggcuu ugacauuaag gccaucgauc uggaccacau ugaggagauc     1140

gcgagccuua uuacccugaa uacggaacgg gaggccuucg aacucucccu gagcaacgag     1200

aucgauaaua accuuaaaca guuccugguc gaaaauuuua acgcucucag aaaguguaaa     1260

ccgaauaacu cuuggcauuc cuucagcaag gagaaucugc agcgccugau cacagagcuc     1320

gaacacaccu cugacgagca aaacacaauu cuggaacggc uugggcugaa auuugaucuc     1380

agaagcaagu auuccgaccg ggauacccug cccauccacg agguucugga ggaaauuuac     1440

aacccuaccg ugucuaagag cgugcgccag acauucagag uccuuaaugc ccucgugaaa     1500

aaguacggaa aagagaacau cgacuauguu acgauugaaa ugccccggga uaagaaugag     1560

gacgaccaga aaaagaccau cagagaaauu cagaagguga acaaaaaucg caaggauaaa     1620

uccaaggagu acuuucugaa aaagagcggc uggucugagg aaaaauucga ggaaaagcug     1680

cggacaucca gacgcuuugc uagcaagcuc uauuacuucu augagcaaga cggccugugc     1740

gccuacaccg ggcagcagau ccagccugaa gaucuucuga cagagagagu cgagguggac     1800

cauaucauuc cccucucugu uucccuggau gacagcauca acaauaaagu gcuggucacc     1860

uccgaagcca accaaaagaa aggacagcgg ucuccguaug augcuuuuau gagcggcgac     1920

uucuccaagu accgcacgua cgaugaguau aaagcguggg ugcuuucuaa uaaguacaag     1980

aaauauaaga aagcccuccu gcuggaagag agaaacauuu uugacccuga acucgcuaag     2040

aaauuccggc agcgcaaucu gaacgauaca agauacgcca gccggaucgu ccuuaaugcc     2100

gugcaguccu uuuucuauaa gaccgacacc aagacacgcg uugugaacgg ggcuuucacc     2160

cacacacugc ggaaaaagug gaaucucgcc gauaaagaca gagagacgca ucaccaccau     2220

gcgauugacg cuacccuggu cgccgugaca cccuuuguua aggccgagcg cuacgaauau     2280

uacaaagaua gcgagggaaa ggcuucuaug guggacaccg ucacaggcac caucaucccu     2340

uacuccgaau auaagaaaaa gaaccuguac gauagaagca acuaucuucu cauguucggg     2400

aaugagucua cgauuaacca agagaaagaa gacaaguccg ccgaguuuau ccggaaucug     2460

auucccgcua aaaucuaccc cgaaauuaag uucagccacc agguggauag aaaaucuaac     2520

cgccggcuga gcgacgccac caucuauucc acaagaagcu acaccgugga gaagggccgc     2580

ggaaagaaua aacgggaagu uaucgagaga gauauuaucg gcaagauuaa agacaucuau     2640

acagcggaug gguacgccga guacgucaag uauaaagacu cucuccuggu gaaggaaauu     2700

gaugagaaga ccuuugaaaa acuugaggcu aucaucgaag aguucuccga cacgguggag     2760

gucacagacc agcaaggaaa ggugaaaaag guuagcaaau ccccuuuucu gcaguacugu     2820

gaaaaguaug augugccggg cgucaccaag uacucuaaaa agaacaaugg gcccgugauu     2880

cgcagccuca aauaucggua cacaaagcug agaucccaua ucgacauuuc uccuaaaaac     2940

agcaagaaua ccguugcccu gcuuacccuc gcucccuggc gcacagaugu guauuacucc     3000

caggagacgg ccucuuacca ucugcuggga cucaaguaua ucgaccugag cuuccgggau     3060

ggcggguacg gcgucuccag aaaacgguau gaagagauua agaaaaacga acgcaucccu     3120

gaggacggag aguuuaaguu cagccuuuac aaaaaggauu auauugaaau cgaguuugac     3180

aagaaaaucc ugcccggccu cuaccagucu agagaugugu ccgcgcggaa uaaguucagc     3240

augaaaccga uugacaaggc ccaauggacc ucuaaagaag auguucugau guuugggaac     3300

gugaaggcuu ccggagucuu cgagaaaaca cugaagaaga auuaugugcu ucgcaaauac     3360

cacguggacg uccugggcaa caaguucgag guuaaagaug aaauccucaa ggacauucug     3420

gaguga                                                                3426


<210>  4009
<211>  3429
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4009
auguacagcg ugggccugga caucgggauu gccuccugcg gauauucuau cauuaacgau       60

gucaauggca acaucguuga gcucgggacc cugcuuuuca agagcagaaa uuccgacaac      120

aaugaagaac ggcgcacaaa cagaggaucu cggcgccuga uuaauagacg gcgcacgaga      180

cucgaugacg gcaaaaagcu gcuugcugaa aaaggguuua agcaggauag caaacugaag      240

cacguguguc ccuacgagcu ccgggugaag ggacugcaug aaaaacugac ccaaggcgag      300

aucuauaagg ucgugcucca cauccugaaa aagcgcggca uuuccuaccu ugacgagucu      360

gaugaaaaca gcuccgggau cgacggagcc agcuauaaag aucagguuaa ucugaaccuc      420

aaucugcaga aggagcuuac accuggccag auucaacuga agagacucaa agaaaacggg      480

cggaucaagu cuggagugac cuucgagaau ggcuaccagc ugaacgucuu uuccgugagc      540

gcguaugcua augagcugaa caaaauucuc acaaagcagu cugaauacua cuccgagauc      600

gaccagauuu ggaucgaucg cuucaccaaa aacggguaua gaaaggaagc cggacugguu      660

uaccggaaac gcccguauua cgacggcccc ggcaaugaga agaacaauag caucuauggg      720

agaugggcca acuuuaagga agauggacau ccuaaagaga auauuuucga ccaacuuauc      780

ggccuggacu cuguggauuc cacacagguc cgggcuagcg ccuccacccu cacggcucag      840

aaguacaacc ugcuuaauga ccugaacaau cucaccauua ucgaggauga cagaaaauuu      900

acaaccgaug aaaagguguc uauuaucaac uaucugauga cacaggacau cacccgcuuc      960

gggcccaaag agcuggccaa guuuuaccaa cucgaagugg aucagauucg gggauggaga     1020

aucgacaaua agggcaaccc ugagauucac agccugaaag ucuaccgcga uuggaagaaa     1080

aucuucaaua cguccagcau ugaccuuaca ucuauccaga ccuggguugu ggaugaacug     1140

gcgaacguga ucacacucaa uaccgagauu cccuccaccc uggccacacu uaagaucaaa     1200

auuccugaca ucgaccaaca gauucugaac uuugucgaga acaauuucua ugaacucaag     1260

aagaaaacga gcaagaacuc uuggcauagc uuuucccuga aaacccugaa uauccucauu     1320

ccggagcugc uuaacacauc ugaagaacag aauaccaucc uggaacggcu caaccugaag     1380

uucgaucuga aaaauaagua cgcugagaag aaccagaucc ccgugaaaga gcuucucgaa     1440

caaauuuaua auccuacagu uaccaagagc gugcaccagu ccaucaaacu guucaacgag     1500

auuaucgaaa aguacgggaa aaauaacauu agcuauguca cgaucgagau gcccagagac     1560

aagaaugauc aggaccagaa agauacaauc aagcgcauua acaagcucaa uucugagcgg     1620

aaaaacgacu ccgaaaagua cuuucuggag agaagcggau gggauaccau ccgcuucgaa     1680

ucugcccuga aaaagccucg guuugcugag aaacuucugu ucuauuacga acaaaagggc     1740

aagugcgccu acuccggcca ggacauucuc gcggagagcc ugcugaacga guccugugaa     1800

guggaucaca ucauuccccu uucuaucagc uuugacgauu ccauccauaa uaaaguucuc     1860

gugaccgccu cuagcaacca ggagaagggg cagagaacac cguauauggc uuucauugac     1920

ggagccaaac ugggccaauc cugggaugac uuugucgcuu gggugaauag cugcggguuc     1980

caccgcaaga aaguccagaa ccugcucuac gaagagaaua ucuuuaaccc ugacgugcag     2040

cggggauucg uuucuagaaa ucugaacgau acccgguaug ccucccgcau ugugcuuaau     2100

gccauccaga gcuuuuucua caagcaaaag gaccugacaa aagucaaggu gguuaacggc     2160

ucuuuuacgc auacccuccg gaaaaagugg ggguccagcc uggauaaaaa uagagacaca     2220

uaucaccacc augcugugga ugcgacccug ugugccguca cacccuucau uaacaucgug     2280

aaguacgagu ucuuugagga ggacggcauc aaguauaugg uggaucuuac cacgggagaa     2340

aaaguugcuu acucugacua caagaaaaug aaguucuaug auaaaagagc cuacgucccu     2400

auuugggacg gcuuuaucaa gcagcucacc cccgccaauc uguaucccaa aauuaaguuc     2460

agccaccagg uggauuccaa guggaaccgc aaaaucagcg acgcuacaau uuacucuacc     2520

cggaagaucg uggagacaaa aauuaauaag aaaggggucg ccaaggaaac cgugaaggau     2580

aucguuaucg acaaaauuaa ggacaucuau acgccugagg gauggagaaa auuuaaggaa     2640

aaacaagaua agcugcucau gaagaacuuc gacgagaaaa cauacaacaa gcugauuaaa     2700

aucgcgaagg aguauccgga gaaaaaggag gugcagaccu ccaauggcaa ggucagccuu     2760

guggaaguuu cccccuuuaa aauguacugc gaggagcaga acgugccugc uauucgcaag     2820

uacucuaaaa aggggaaugg acccuauauc agaagccuga aauucuacga uaaggaacuc     2880

ggcaagcaua ucaacauuac aaaagacucc uaugggaaug auaucgagcg gaccauucgc     2940

aacaagaaaa ucauucugaa gaaucagaac ucuuggagaa ccgacgucua cuauaauagc     3000

gugaacaauu guuacgaacu gcuuggcauc aaauauugcc accucaaguu uuaccgggga     3060

aaauacggca ucccuaaguc cauuuaugau gagcugaaga aagaagacga gaucucuagc     3120

gaguccguuu ucaaguuuag ccuguaccgc caugauagag ugcgguucga aaaagacggg     3180

gaguauauug aagcccucuu uggaucuaag aacgagucca auaaaaacua ccuggaacuu     3240

agccccaucg auaagaauaa guggcugucu aaagagaacg ucaaguucuu cggcgaggug     3300

gccgcuuccg ggcgcuuuau uaaaggacuc aagccgggca ucaaagugau uaagcaaagc     3360

acagacuaua uggggcacgu cuacgccauc caccaggaaa agcugaaauu cguucugucu     3420

gacgcuuga                                                             3429


<210>  4010
<211>  3435
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4010
augaccagca agcaggagaa acacggcgcc cugacauucg ggcucgacau cggaauugcu       60

uccgugggcu gggccguccu gggggaaucu agaaucguug aucuuggagu gcggugcuuu      120

gacaaggcgg agacggcuaa agaaggcgag agccugaacc ucacccgcag aauggcccgg      180

cugcuucgca gacggcugcg cagacgggcc uggcgccuca caaagcuggc uagacugcuc      240

aaacgggaag ggcugauugc cgaugcuaau cuuuucaagc auaagaucga gaaaaacacc      300

cccgugccua aguccucuug gcaacugcgc gucgacggac ucgauagaca gcugaaugcc      360

gaggaauggg cgcgggugau uuaccaccuu uguaaacauc gcggcuuuca cuggaucagc      420

agagccgagg aaaaggcugc cgagggcgac uccaaagggg agggaggcaa gguuaagcag      480

gggcuggcug gaacgaaaaa gcucaugcag gaaaaaucuu aucggaccgc cgcggagaug      540

gugcuggccg aguucccgga ugcucaaaga aacaagcagg gcgacuacag caaagcccug      600

ucccgcaucc uccuggggga ugaacuuaag gcucuguuug aacaucagcg gaaacucgga      660

aaucccgucg ccacagagaa gcuggaggcc gcuauucuug gcaacggcga caagaaaugc      720

gggcuguucu gggcgcagaa uccugcccuc gcuggaaaca agcugcugga aaugcucggc      780

agauguaccu uugagaaaac agggggagau gacggcaagg gggaauaucg cgcccccaaa      840

gccagcuucu cugcugagcg gcacgugugg cugacccacc uuaauaaccu gagaaucguu      900

guggauggaa caacccgccc ucucaaugaa gccgagcgga gagucgcgcu gcccaugccu      960

uaccaacagg cuggcgaccu uuccuauaag cagcugggcg gggcccucau uaaggaggga     1020

uuucugcaga aagacggcuu caaguuugcc gggcugccgu accccacaaa acaagcuagc     1080

gaagagaagg ccaaagaucc ugaaaccucu gugcucugca agcugcccgc uuggcauacg     1140

cuucgcaaga cacuggagaa acacggacuc gaauccgagu ggcagaagcu gagcaccgac     1200

gcccuggaug gcaaaccuca gcuucucgac aacaucgcgu gggugcuguc ugucuauaag     1260

gaugacgaug agguuaaaac agaacugggg aagcucaauc ugcccaacac cgagaagaug     1320

auugacgccc uucuggauau ccgguucgac aaauuuucca aucucagccu gaaggcucug     1380

uccaaaauug ugccguucau ggaacaaggc cagagauacg augaggccgu ggcuaacauc     1440

ccugaguacg gacaucacuc ucagcuucac aaggucggcg ccggggaaca uaaauaucug     1500

cccccuuucu acaaggagcg cgacaaaacc ggacggaugg uguuuaacga cgauauggac     1560

auccccagaa aucccguugu gcuccgggcg cugaaccagg cccgcaaggu cgugaaugcu     1620

cucauuaagg aauauggcag cccucacgag guucauaucg aaauggccag agaucugucc     1680

cggccgcuug acgagagacg cgauauugag aaacugcaaa aggaguucgg ggaacggaac     1740

aaaaaggaca aagaacucuu ugcucaggau uucaauaucg ugggaacagu caagggcaag     1800

caguuugaga aauaccagcu guauagagaa caacagggga aguguauuua ccccagcaug     1860

gacgagaaac agggcaucug cuguuauucu cugaagccuc uugaucucca ccgcgugcug     1920

uacgaggaag gauacgccga gaucgaccau gcccugcccu auucccggag cuucgaugac     1980

ucuaaaaaca auagaguccu cgugcuggcu aaggaaaacc gcgauaaggg caauagaacg     2040

ccuuuugagu accuugggga caaagacaac agcgaacccu ggcggcaguu cguugcguuu     2100

guggagggaa auaaggccua ucgccuggcu aaaagauccc ggcuccugcg caaggauuuc     2160

ggcgacaaag aggccaagga auuuagagag cggaaccuga augauacccg cuacauuugc     2220

aaguucuuua aaaacuaugu cgaacaauac cuucagcucc acgaggacuc ugaggccaag     2280

agaugugugg uucugagcgg gcagaugaca uccuuccugc gggcuagaug gggacucgug     2340

aaaguccgcg gcgauagcga ccggcaccau gcccuggaug cggcuguggu ggccgccugc     2400

ucucacggga ugguuaagag acuuuccgac uauagccgcc ggaaagaacu ggagcagguc     2460

agagaaggcu ucguggaugu ggacaccgga gagaucguca auccggcuau guuucaacag     2520

gugcaggaac auuucccuaa ccccuggccu cacuuucgcg augagguuaa gcuccggcug     2580

ucuauugacg aucccacacu gcuuagaucc gagauggaaa aauucggcac cuacacgccu     2640

gagagccugg aagcccuccg cccccuguuu gugucccggg cgccgcaaag acggaacucu     2700

ggggcugccc acaaggacac aaucuacgcc cagccuaagc gccucgucgc ucagggaggc     2760

gugacccaga aaguugcccu gagcucccuu acccugaagg accucgauaa acugauugac     2820

ccccauagaa augagcggcu guaugcugcc auccgcgcga gacuugaaca acacggggga     2880

aagggcgaua aagccuuccc cgcuucuaac ccucugcgga agccggacag cgaggggaau     2940

cccacaggcc cuaucgugag aggagucacc auggugauug auaagcucuc cggcaucccc     3000

auucgcgggg gaaucgccaa aaacgacagc augguucggg uggaugucuu uacaaaggcu     3060

aauaaauucc aucuggugcc uguguacguc caccauucug uucucggccu gcccaaccag     3120

gccauugucg ggggaaagga ggaauccgag uggacgcaga ucgacgauag cuuugacugg     3180

cacuucucua uuuauccuaa ugaucuuauc agagugaccc ugaaacagga aggccgcuuu     3240

ggcuacuaug ccggguguga ccgguccaca gcugcgaucc accucugggc ccaugacaga     3300

aaccaacagg ugggaaagga uggccugauu cgcagcaucg gggugaagac cgcucugucu     3360

auugagaaau ucaauguuga cgugcuugga aaccgguacc ccgccccgcc ugaaaagaga     3420

cgcgaucugg ccuga                                                      3435


<210>  4011
<211>  3435
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4011
augaccagca agcaggaaaa ccugggcgcc cucacauucg ggcuggacau cggaauugcu       60

uccgugggcu gggccguccu uggggaaaau agaaucauug aucugggagu ucggugcuuu      120

gacaaagcgg agacggcuaa ggaaggcgag ucucucaacc ugacccgcag aauggcccgg      180

cuucugcgca gacggcuccg cagacgggcc uggcgccuga caaaacuggc uagacuccug      240

aagcgggaag ggcuuaucgc cgaugcuaau cuguucaaac acaagauuga gaagaacacc      300

cccgugccua aaagcuccug gcaacuccgc guggacggac uggauagaca gcuuaaugcc      360

gaggaauggg cgcgggucau cuaccaucug uguaagcacc gcggcuuuca uuggaucucu      420

agagccgagg aaaaagcugc cgagggcgac agcaaggggg agggaggcaa agugaagcag      480

gggcucgcug gaacaaagaa acugaugcag gaaaaguccu aucggaccgc cgcggagaug      540

guucuggccg aguucccgga ugcucaaaga aacaaacagg gcgacuacuc uaaggcccuc      600

agccgcauuc ugcuugggga ugaacugaaa gcucucuuug aacaccagcg gaagcuggga      660

aaucccgugg ccacagagaa acuugaggcc gcuauccugg gcaacggcga caagaagugc      720

ggacucuucu gggcgcagaa uccugcccug gcugggaaca aacugcucga aaugcugggc      780

agauguaccu uugagaagac agggggagau gacggcaaag gggaauaucg cgcccccaag      840

gccuccuuca gcgcugagcg gcaugucugg cuuacccacc ugaauaaccu cagaauugug      900

guugauggaa caacccgccc ucugaaugaa gccgagcgga gaguggcgcu ucccaugccu      960

uaccaacagg cuggcgaccu gucuuauaaa cagcucggcg gggcccugau caaggaggga     1020

uuucugcaga aggacggcuu caaauuugcc gggcucccgu accccacaaa gcaggcuucc     1080

gaagagaaag ccaaggaucc ugaaaccagc guccugugca aacuucccgc uuggcacacg     1140

cugcgcaaga cacucgagaa gcauggacug gaaucugagu ggcaaaaacu guccaccgac     1200

gcccuugaug gcaagccuca gcuccuggac aacauugcgu gggugcugag cguguauaaa     1260

gaugacgaug aggucaagac agaacucggg aaacugaauc uucccaacac cgagaagaug     1320

aucgacgccc ugcucgauau ucgguucgac aaguuuucua accugucccu gaaagcucuu     1380

agcaagaucg uuccguucau ggaacaaggc cagagauacg augaggccgu ggcuaauauc     1440

ccugaguacg gacaccauuc ccagcuccac aaagugggcg ccggggaaca caaguaucug     1500

cccccuuucu acaaagagcg cgacaagacc ggacggaugg ucuuuaacga cgauauggac     1560

auucccagaa aucccguggu ucugcgggcg cucaaccagg cccgcaaagu ggucaaugcu     1620

cugaucaagg aauauggcuc uccucaugag gugcacauug aaauggccag agaucuuagc     1680

cggccgcugg acgagagacg cgauaucgag aagcuccaaa aagaguucgg ggaacggaac     1740

aagaaagaca aggaacuguu ugcucaggau uucaauauug uuggaacagu gaaaggcaag     1800

caguuugaga aguaccagcu guauagagaa caacagggga aauguaucua ccccuccaug     1860

gacgagaagc agggcaucug cuguuauagc cuuaaaccuc ucgaucugca ucgcguccug     1920

uacgaggaag gauacgccga gauugaccac gcccuccccu auucuagauc cuucgaugac     1980

agcaagaaca aucgggugcu gguccuugcu aaagaaaacc gcgauaaggg caauagaacg     2040

ccuuuugagu accuggggga caaggacaac ucugaacccu ggcggcaguu cguggcguuu     2100

guugagggaa auaaagccua ucgccucgcu aagagaagcc ggcugcugcg caaagauuuc     2160

ggcgacaagg aggccaaaga auuuagagag cggaaccuua augauacccg cuacaucugc     2220

aaguucuuua agaacuaugu ggaacaauac cuccagcugc augaggacuc cgaggccaaa     2280

agaugugucg ugcugucugg gcagaugacg agcuuccucc gggcuagaug gggacugguu     2340

aaggugcgcg gcgauuccga ccggcaccac gcccuugaug cggcugucgu ggccgccugc     2400

agccauggga uggugaaaag acugucugac uauucccgcc ggaaggaacu cgagcagguu     2460

agagaaggcu ucgucgaugu ggacaccgga gagauuguga acccggcuau guuucaacag     2520

guccaggaac acuucccuaa ucccuggccu cauuuucgcg augaggugaa acugcggcug     2580

agcaucgacg aucccacacu ucugagaucu gagauggaaa aguucggcac cuacacgccu     2640

gagucccucg aagcccugcg cccccucuuu guuagccggg cgccgcaaag acggaacucc     2700

ggggcugccc acaaagacac aauuuacgcc cagccuaagc gccugguggc ucagggaggc     2760

gucacccaga agguggcccu uucuagccug acccucaaag accuggauaa gcugaucgac     2820

ccccacagaa augagcggcu uuaugcugcc auccgcgcga gacuggaaca acauggggga     2880

aaaggcgaua aggccuuccc cgcuuccaac ccucuccgga aaccggacuc ugaggggaau     2940

cccacaggcc cuauuguuag aggagugacc auggucaucg auaagcugag cggcauuccc     3000

auccgcgggg gaauugccaa gaacgacucc auggugcggg uugauguguu uacaaaagcu     3060

aauaaguucc accucguccc uguguacgug caucacagcg uccugggccu ucccaaccag     3120

gccaucguug ggggaaaaga ggaaucugag uggacgcaga uugacgauuc cuuugacugg     3180

cauuucagca ucuauccuaa ugaucugauc agagugaccc ucaagcagga aggccgcuuu     3240

ggcuacuaug ccggguguga ccggucuaca gcugcgauuc accugugggc ccacgacaga     3300

aaccaacagg ugggaaaaga uggccugauc cgcuccauug gggucaagac cgcucuuagc     3360

aucgagaagu ucaaugugga cguucuggga aaccgguacc ccgccccgcc ugagaaaaga     3420

cgcgaucucg ccuga                                                      3435


<210>  4012
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4012
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc ccuugugaaa       60

gagacggaaa auuccaacga gaagucugaa aucauuaaac ugggcgucag aguuaauccc      120

cucaccgugg augagaagac aaacuucgaa gcugggcggc cucugagcac caaugccgac      180

cgcacagcga aaagauccgc ucggcgcaac cuucagagau acaagcugcg gcgcaagaau      240

cucaucgauc ugcugauuaa acacagacuc aucgacaagg auaccccgcu gacagagauc      300

gggaaaaaca ccacgcauca aacccuugag cugcgggcca aggccgcucg cgaaagaauu      360

gagcucgaag accuggcccg gguguuucuu gcuaucaaua aaaagcgcgg cuauagaucu      420

agccggaagg ucaacaauga ggaggaagga caggugguug auggaauggc cguggcgaaa      480

aagcuguacg acgagaaccu cacacccggc caguaugccu acgaacugcu guccaaaggc      540

aagaaauaug ucccugauuu cuaccgcucu gaccuccagg cugaguuuga uagcauuugg      600

gaauaucaaa agcaguucua cgccgacauc cuggaugacg agcuuuacaa ugcucugaaa      660

gggcagggac agcaaaacuc cagaaagcgg uuucucgcca uuaagggcgu guauaccgcg      720

gagaauaaag ggaagagaga cgaaguuaaa cugcagcacu acaaguggcg cagcgaggcc      780

aucacacaga aacuuucuau ugaagaggug gcuuaugucc ugguggaaau caacaacgau      840

cucaauaagu ccagcggaua ccugggcgcc aucucugacc gguccaagga gcuguauuuc      900

aacaaagaga ccguggggga aaaucucugg aagcagauuc agaaaaaccc ccauacgagc      960

cugaagaauc aagucuuuua cagacaggau uaucuugacg aguucgaaca aaucugggag     1020

acacaggcuc aguuucaccc ucagcugacc cucgcccuga aagaacaaau ucgcgauguu     1080

gugaucuucu accagcggaa acuuaagucu cagaaaggac ugcucuccuu uugccaguuc     1140

gagagcuggg agauugaaag aaaggacgag aacggcaaug ugauccugaa caaaacaacc     1200

caacugccca agcgccagac cgucggccgg agaguggccc cuaaauccuc uccgcucuuu     1260

caggaguuca agaucuggca gaauauuaac aaucuggaaa ucgcuaagau uagcgauggg     1320

aacuccaaaa auaagaaaga gacagaagcg cuuagcgacg augagcgcaa gcuccuguuc     1380

gaggaacuga accugcgggg aaaucuuucu caaaaagaag uucuccagau ccugggccug     1440

aaggacaaag aguacaagac gaacuuuccc gaggggcucg aaggaaacag aaccaaugcc     1500

gcucuguuca acauuuauca gcagaucgcc gagaaugaag gcuacgggga uuggacaaag     1560

aaauccgccc aagagaucaa ggaggaacuu aaagcugugu uuccucagau uggaauccag     1620

gccgacauuc uggacuucaa cgcggagcuc gauggcaagg aauuugagaa ucaagcuagc     1680

uaucagcugu ggcaucugcu uuacucugcc gaagaggacg auaaaaucaa cgaagaggac     1740

cagauuaucu auggcaauag cgccgucucc cucaagaaga aacuguguga gaaguucggg     1800

uuuacccccg aauacgcuaa auggauugcc aacgugucuc ugcaggauga cuauggaaau     1860

cucagcacaa aggcuaugcg caaaaucauc ccuuaccuga uugauggcaa cgacuacucc     1920

gaggccugcg cgcuugccgg guauaaucac agcaacucuc ugaccaagga agagaaugau     1980

aaccgggaga gacucaauaa gcuggaacug cuucccaaaa acucccuccg gaacccgguu     2040

guggagaaga uccugaauca aauggucaac guggucaauc aggugauuga aacguacgga     2100

aaaccugacg agguucgcau cgaacuggcu agagagcuca agaaaagcgc cgaggaacgg     2160

gcugagauga caaagggcau uaacgaagcc accagacgca augaggauau caagaaacug     2220

aucaccaaga acuucgggau ucccaauccu accaaaaacg acgugguccg guauagacuu     2280

ugggaggaac uggcgccccu cgccuacaag gauguguuua caggccgcca gaucaaaaag     2340

gaggaccugu ucucuuccaa aauugacauc gaacacauua uccccaaggc ucugcuuuuu     2400

gaugacagcu ucuccaauaa gacacucgcc uuuagagaga cgaaccugaa aaaggcugau     2460

cggaccgccu auggauucau cgaaucugac uacaaugcca cacuggauga cuauauucag     2520

cgcguugaga cccucuacaa caaugcuaaa ggcacaauca gcaaggggaa aagaaacaag     2580

cugcuuaugg cgcaaaagaa ucugccugau ggauucauug aacgggaccu ccgcaacucc     2640

caguacaucg ccaaaaaggc uaaaucuaug auugagaagg uguuuaacga ugucaaugug     2700

accagcggcu ccaucacgga cagacugcgg gaggauuggg accugaucaa cgugaugaaa     2760

gaacuuaauu ucccgaagua uaaggcccug gggcucaccg agauugagga acgcuacgau     2820

aucggacagg agaaaacaaa gaaaguugaa gucauuaccg acuggacaaa gagaaacgac     2880

caucggcacc augccaugga ugcucugacc guggccuuua cgacaaaaag ccacgugcag     2940

uaucucaaua accugaauac cauccacucu cuuaagggcg acgaggucga ucuggaacuc     3000

uccaagcugu acgggcugaa aaacacaauu accgaggugg uuaauaagaa acgcaaguuc     3060

auucccccua ugcccaacuu uagagaagag gcgaaaaagc auaucgagac cauccuugug     3120

agcaucaaga auaaaaacaa ggucuauaca aagaauauua acaagacgaa aaaggaaucu     3180

ggcuaccaca ccaaagagca acugacaccu cggggacagc uccauaagga aaccguguau     3240

ggcaagucca aaagacccau gaauaagccg acaaaaauca acaagaauuu cagccuggag     3300

caggcucaac ucauuaucaa cguucaggaa aaagagcugg ugcuucgcca ccuggcccag     3360

uuugacaaca auccugccgu cgcuuucgau accaagacgc ucaagaaaug ucauuaccug     3420

aagaugguga accuguga                                                   3438


<210>  4013
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4013
augagcggca acacccacgg gccccugaca uucggauuug acaucggcau ugccuccgug       60

ggguggugcg uccucggaga gaauagaauc guuaaccugg gcgugcggug uuucgauaag      120

gcugaaacgg ccaaagaggg ggaaucucuu aaucuggcgc gcagaaccgc ucggcucaug      180

cgcagacggc ugcgcagacg ggccuggcgc cuuacaaagc uggccagacu ccugaaacgg      240

gagggacuga uugcugaagc cagccgcauc cauauuccuu ccccgguguc ucccuggaga      300

cuccgggucg agggcaucga ccgcagacug accgaugacg agugggcucg ggugaucuac      360

caccuuugca agcauagagg cuuucacugg auuagccgcg ccgaagaacg gaaagcggaa      420

uccgaugcca aggaaggggg aagaguuaag aaaggccugu cugacacaca gcgccggaug      480

accgagaagg gguauagaag cgcugccgaa auggugcucg cugaguuccc ugaugcccaa      540

cgcaacaaac agggagacua cggcaaggcg cugucccgga uccuucugag cgaugaacuc      600

gcccugcugu uuagacgcca gcgggagcuc gggaauccca acgcuggaaa ugaacuggag      660

gccacaauuc uuggcaacgg caauagaaaa ucugggcugu ucugggagca gaagccuccc      720

cucgcuggag ccgaccugcu uaagaugcug ggccgcugua ccuuugagaa aaacgaguau      780

cgggccccua aggcuuccuu cacggcggaa agacaugucu ggcucacccg gcugaauaac      840

cugcgcgugg uuguggaugg gacaaccaga agccucgacg augccgagca ccggcugguc      900

cuuccgcugc ccuacaaaca agcuggagac uuuacauaua agcagcucag aaccgcccug      960

acgaaagccg gccuucugcc ugaaggguuc aaguuugcug gacucucuua ccccacaggc     1020

cgccagcugg acgauccuaa agccaaggac cccgaguccg agaagcuggu gcggcucccg     1080

gcguggcagg aacugagaaa aacccuuaag gauaaaaauc uggagacaga augggagggc     1140

auggcugggg ccgccaccgg aggcaacccu gaacuccugg acgagaucgc ucgcgugcug     1200

agcgucuuca aggaugggga cgagguuacc gccgaacuuu ggaaacuccc ccugccugga     1260

ggcaaggcua ugauugaugc ccugucugag aucuccuuug acaaguucca cgcgcucagc     1320

cugaaagccc uuagaaagau ugugccccau auggaauccg ggcugcggua ugaugaggcu     1380

ugcgccgugg cuggcuacca ccauucucaa cuccacgccg cgggagccgg cgagcacaaa     1440

uuccugcccc cuuuuuacag ccgcagagac aaggaugggc ggauggucuu caaugaggag     1500

cuggacgugc cgcgcaaccc cguugugcuu agagcucuca accaggcccg gaaaguccug     1560

aaugcucugg ugcgcacaua uggauccccu acggccguuc auaucgaaau ggccagagac     1620

cucagccggc cccuggauga gcgcaacaag gugaagaaag cucaggacga auacagagag     1680

cggaaugaga aggauaaagc ggccuuugcu gccgaauaug gcaucgcccc uaagucuaga     1740

gaguucgaaa aauuucagcu uuaccgcgag caacagggga agugugcuua uucccucaag     1800

agccuggacc ugcaccgggu ccugcaugau auuggauacg ccgaagugga ccacgcgcuu     1860

cccuauucua gaagcuacga ugacuccaaa aacaauaagg uccucgcucu gaccgaggag     1920

aaccgcaaua aaggcaaccg gacagccuac gaauaucuga ccucuuuccc gggaggcgag     1980

aauggagaaa gauggcgcaa cuuuagcgug gccguugaga caaauaaggc uuaccggcuc     2040

gccaaaagaa cccgccugcu ucggaaggau uucuccggca gagcggcuga agaguuucgg     2100

gagcgcaacc ugaaugacac gagauauauc ugccgguucu uuaaaaacac aguggaacgc     2160

uuccucaagc uggaugccaa ggucggggcc ggagacaccg cugagauuag cgccaaaaga     2220

ugugugguuc ugucuggcca gaccacagcu uuucuucggg ccagaugggg gcuccugaag     2280

gugcgcggaa auuccgaucg gcaucacgcg cuggacgccg cugucguggc cgcuugcagc     2340

cacggcaugg ugaaaagacu cggggacuac ucucgcacca aggaacuggc caaaguucau     2400

gauggcuucc cugaccccga gacaggagag aucauugauc cugucauguu ccaacagcuu     2460

cacgcccauu uucccgaccc uuggccccac uuccgggcug aacuggaggc gagacucaac     2520

guggaugaca cggccgaucu gcgcgcucgg auggaacugu uuggcaccua ugccccggag     2580

gcccuugaaa cacugagacc ucucuucgug ucccgcgcuc cccagagacg gaacagcggg     2640

gccgcgcaca aggacaccau cuacgcucag cccgagcgcc ugaaggagca aggaggcguc     2700

acacagaaag ugccucucac cucccugacg cuuaaggauc uggacaaacu caucgauccg     2760

cauagaaaug aaaagcugua ugccgccauu cgggcucgcc uggaggccca cgggggaaaa     2820

gcggacaagg cuuuucccgc cggcaacccu cuuagaaagc ccgaucggga agggaauccu     2880

accggccccg uugugagaac agucaccaug gugaucgaca aacugucugg gauuccuguu     2940

cgcggcggga ucgccaagaa cgacacaaug cuccgggugg auauuuucag acauaaaaag     3000

gacggaaaau uucaccuggu ccccguguac guuuaccaug cuguggccaa ggaguucccg     3060

aaucgcgcua ucguccaggc caaggaugaa gacgagugga cccucaucga ugacaaauuu     3120

gauuucuguu uuagcgcgca cccuaacgac cugcuuaaga uuacgcagaa auccggcgag     3180

acaaucuucg gguauuaccg gggaugccac ucuggcaccg ccgcuauuaa uaucgcccug     3240

caugauagau augcuuacgc cgaaaagaca accagccucg ccuuuuccaa aaagaacccc     3300

ggcaaaccua uucccaauga caagcugggg cugaucgagg gaauuggcgu gaagauggcu     3360

gcgaacaugg aaaaauucca cguggauacc cuugggaaua ucuauccggc cccucccgag     3420

caacggcgcg gacuggcuug a                                               3441


<210>  4014
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4014
auggccuaca gacugggccu cgacaucggg auuaccagcg ugggaugggc ugucguugcc       60

cuggagaagg augaauccgg ccuuaaaccc gugcggaucc aggaccuggg ggugcgcauu      120

uucgauaagg cggaggacuc uaaaacagga gcuagccucg cccugccuag acgggaagcc      180

cgcuccgcua gacggcgcac gagacggcgc agacaccggc uuuggcgcgu caagagacug      240

cucgagcaac auggcauccu gucuauggaa cagauugagg cccuguaugc ucagcggacc      300

agcuccccgg auguguacgc ccuccgcguu gcggggcugg acagaugccu uaucgccgag      360

gaaaucgcuc gggugcugau ucacaucgcc cauagacgcg gauuucaguc uaaccggaaa      420

agcgagauua aggauuccga cgcuggcaag cuccugaaag ccguccaaga aaaugagaac      480

cuuaugcaga gcaagggcua uagaacagug gcggagaugc ugguuucuga agccaccaaa      540

acagaugcug aggggaagcu cgugcacgga aagaagcaug gcuacgucuc caaugugcgc      600

aacaaggccg gggaauaucg gcacaccgug agcagacagg cuaucgucga cgagguucgc      660

aaaauuuucg ccgcccagcg ggcucuggga aaugauguga ugucugaaga acuggaggac      720

uccuaccuca agauccugug uagccaaaga aacuuugaug acggccccgg gggagacucu      780

ccuuauggcc acggcuccgu gagccccgau gggguccgcc aguccauuua cgaacggaug      840

gugggaucuu gcacauucga gaccggcgag aaaagagcgc cucgcagcuc cuacagcuuu      900

gagcgguucc agcuucugac gaagguugug aaucucagaa ucuaucggca gcaagaagac      960

gggggacgcu accccuguga gcugacccag acagagagag cccgggucau cgauugcgcu     1020

uaugaacaga ccaaaauuac auacggcaaa cuuagaaaac ugcucgacau gaaggauacc     1080

gagucuuuug ccgggcugac guauggacug aaccgcuccc ggaauaagac agaagacacc     1140

guguucguug agaugaaauu uuaccaugaa gugagaaagg cccuccaacg cgcuggcguc     1200

uucauccagg aucugagcau ugagacacuu gaccagaucg gcuggauucu gucugugugg     1260

aaaagcgaug acaacagacg gaagaaacuc uccacccugg ggcuuucuga uaaugucauc     1320

gaggaacugc ucccucugaa cggaagcaag uuuggccacc ugucccucaa ggccauccgc     1380

aaaauucugc cguuccuuga ggacggguau agcuacgacg uggcguguga acuggcugga     1440

uaccaguuuc aaggcaagac cgaguauguu aaacagagac uccugccccc ucugggggag     1500

ggcgaaguga caaaucccgu cgugcggcgc gcccuuucuc aggccaucaa gguugugaac     1560

gcugucauua gaaaacaugg auccccugag agcauccaca uugaacucgc ccgggagcug     1620

ucuaagaauc uggaugaacg cagaaagauc gagaaagcuc agaaggagaa ccagaaaaac     1680

aaugaacaaa ucaaggacga gauucgggaa auccucggcu ccgcccacgu gacggggcgc     1740

gauauuguga aauacaagcu guucaagcag cagcaagagu ucugcaugua uagcggagaa     1800

aaacuugacg uuaccagacu guuugagccc ggcuacgcgg aggucgauca uaucauuccg     1860

uaugggaucu ccuucgacga uucuuacgac aacaaggugc ucgugaaaac agaacagaau     1920

cggcagaagg gaaacagaac cccucuggag uaucugcgcg auaaacccga acagaaggcc     1980

aaauuuauug cucuugucga gagcaucccu cucucccaaa agaagaaaaa ucaucugcug     2040

auggacaagc gggccaucga ucucgaacag gagggcuuca gagagcgcaa ccugucugac     2100

acacgguaca uuaccagagc ucuuaugaau cacauccagg ccuggcugcu cuuugaugaa     2160

acggcgagca cacgcuccaa acgggugguu ugugugaacg gggccgucac cgcuuacaug     2220

agagcccgcu ggggccugac caaggaccgg gacgcuggag auaaacacca ugccgccgac     2280

gcugugguug uggcgugcau uggcgauagc cugauccaaa gagucacaaa guaugacaag     2340

uucaaacgga augcccuugc ugaucgcaac agauacgugc agcagguuuc uaaguccgag     2400

gggauuaccc aguaugugga caaagaaaca ggagaggucu uuacguggga gagcuucgau     2460

gaacggaagu uucuccccaa ugagccccug gaaccuuggc cguucuuucg cgacgagcug     2520

cucgccagac ugucugauga ccccuccaaa aacauccggg ccaucggccu ucugaccuac     2580

agcgaaacag agcaaauuga uccuaucuuc gugucuagaa ugcccacccg caaggugaca     2640

ggggcugccc acaaggagac cauucggagc ccuagaaucg ucaaaguuga cgauaauaag     2700

ggaacggaaa uucagguggu ggucuccaaa guggcgcuca ccgagcugaa gcugacaaaa     2760

gacggcgaaa ucaaggacua uuuucgcccc gaggaugacc cgcggcuuua caacacccuc     2820

agagaacgcc ugguucaguu cgggggcgau gcuaaggccg ccuucaaaga gccuguguau     2880

aagaucagca aagacggauc uguccggaca cccgugagaa agguuaaaau ucaggagaag     2940

cugacccucg gcgugccugu ccacggggga cgcggcaucg cugaaaaugg gggaauggug     3000

agaauugaug uuuuugccaa aggcgggaag uacuacuucg ugcccaucua ugucgcggac     3060

gugcugaagc gggagcuucc uaaccgccug gcuacggccc auaaacccua cuccgaaugg     3120

agaguggucg augacagcua ucaauuuaag uucucccucu acccgaacga ugccguuaug     3180

auuaaaccuu cucgggaggu ggacaucaca uauaaggauc gcaaagaacc cgugggcugu     3240

agaaucaugu acuuugucag cgcuaauauu gccuccgcuu cuaucagccu gcggacccac     3300

gacaacuccg gagagcugga gggccuuggg auucagggac uggaaguguu cgagaaguau     3360

guugugggcc cccucgggga cacacauccu gucuacaagg aaagacgcau gccguuucgg     3420

guggagagaa aaaugaauug a                                               3441


<210>  4015
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4015
auggccuaca gacugggccu cgacaucggg auuaccagcg ugggaugggc ugucguugcc       60

cuggagaagg augaauccgg ccuuaaaccc gugcggaucc aggaccuggg ggugcgcauu      120

uucgauaagg cggaggacuc uaaaacagga gcuagccucg cccugccuag acgggaagcc      180

cgcuccgcua gacggcgcac gagacggcgc agacaccggc uuuggcgcgu caagagacug      240

cucgagcaac auggcauccu gucuauggaa cagauugagg cccuguaugc ucagcggacc      300

agcuccccgg auguguacgc ccuccgcguu gcggggcugg acagaugccu uaucgccgag      360

gaaaucgcuc gggugcugau ucacaucgcc cauagacgcg gauuucaguc uaaccggaaa      420

agcgagauua aggauuccga cgcuggcaag cuccugaaag ccguccaaga aaaugagaac      480

cuuaugcaga gcaagggcua uagaacagug gcggagaugc ugguuucuga agccaccaaa      540

acagaugcug aggggaagcu cgugcacgga aagaagcaug gcuacgucuc caaugugcgc      600

aacaaggccg gggaauaucg gcacaccgug agcagacagg cuaucgucga cgagguucgc      660

aaaauuuucg ccgcccagcg ggcucuggga aaugauguga ugucugaaga acuggaggac      720

uccuaccuca agauccugug uagccaaaga aacuuugaug acggccccgg gggagacucu      780

ccuuauggcc acggcuccgu gagccccgau gggguccgcc aguccauuua cgaacggaug      840

gugggaucuu gcacauucga gaccggcgag aaaagagcgc cucgcagcuc cuacagcuuu      900

gagcgguucc agcuucugac gaagguugug aaucucagaa ucuaucggca gcaagaagac      960

gggggacgcu accccuguga gcugacccag acagagagag cccgggucau cgauugcgcu     1020

uaugaacaga ccaaaauuac auacggcaaa cuuagaaaac ugcucgacau gaaggauacc     1080

gagucuuuug ccgggcugac guauggacug aaccgcuccc ggaauaagac agaagacacc     1140

guguucguug agaugaaauu uuaccaugaa gugagaaagg cccuccaacg cgcuggcguc     1200

uucauccagg aucugagcau ugagacacuu gaccagaucg gcuggauucu gucugugugg     1260

aaaagcgaug acaacagacg gaagaaacuc uccacccugg ggcuuucuga uaaugucauc     1320

gaggaacugc ucccucugaa cggaagcaag uuuggccacc ugucccucaa ggccauccgc     1380

aaaauucugc cguuccuuga ggacggguau agcuacgacg uggcguguga acuggcugga     1440

uaccaguuuc aaggcaagac cgaguauguu aaacagagac uccugccccc ucugggggag     1500

ggcgaaguga caaaucccgu cgugcggcgc gcccuuucuc aggccaucaa gguugugaac     1560

gcugucauua gaaaacaugg auccccugag agcauccaca uugaacucgc ccgggagcug     1620

ucuaagaauc uggaugaacg cagaaagauc gagaaagcuc agaaggagaa ccagaaaaac     1680

aaugaacaaa ucaaggacga gauucgggaa auccucggcu ccgcccacgu gacggggcgc     1740

gauauuguga aauacaagcu guucaagcag cagcaagagu ucugcaugua uagcggagaa     1800

aaacuugacg uuaccagacu guuugagccc ggcuacgcgg aggucgauca uaucauuccg     1860

uaugggaucu ccuucgacga uucuuacgac aacaaggugc ucgugaaaac agaacagaau     1920

cggcagaagg gaaacagaac cccucuggag uaucugcgcg auaaacccga acagaaggcc     1980

aaauuuauug cucuugucga gagcaucccu cucucccaaa agaagaaaaa ucaucugcug     2040

auggacaagc gggccaucga ucucgaacag gagggcuuca gagagcgcaa ccugucugac     2100

acacgguaca uuaccagagc ucuuaugaau cacauccagg ccuggcugcu cuuugaugaa     2160

acggcgagca cacgcuccaa acgggugguu ugugugaacg gggccgucac cgcuuacaug     2220

agagcccgcu ggggccugac caaggaccgg aaugcuggag acaaacacca ugccgccgau     2280

gcugugguug uggcgugcau uggcgacagc cugauccaaa gagucacaaa guaugauaag     2340

uucaaacgga acgcccuugc ugaccgcaau agauacgugc agcagguuuc uaaguccgag     2400

gggauuaccc aguaugugga uaaagaaaca ggagaggucu uuacguggga gagcuucgac     2460

gaacggaagu uucuccccaa cgagccccug gaaccuuggc cguucuuucg cgaugagcug     2520

cucgccagac ugucugacga ucccuccaaa aauauccggg ccaucggccu ucugaccuac     2580

agcgaaacag agcaaauuga cccuaucuuc gugucuagaa ugcccacccg caaggugaca     2640

ggggcugccc acaaggagac cauucggagc ccuagaaucg ucaaaguuga ugacaacaag     2700

ggaacggaaa uucagguggu ggucuccaaa guggcgcuca ccgagcugaa gcugacaaaa     2760

gauggcgaaa ucaaggacua uuuucgcccc gaggacgauc cgcggcuuua caauacccuc     2820

agagaacgcc ugguucaguu cgggggcgac gcuaaggccg ccuucaaaga gccuguguau     2880

aagaucagca aagauggauc uguccggaca cccgugagaa agguuaaaau ucaggagaag     2940

cugacccucg gcgugccugu ccacggggga cgcggcaucg cugaaaacgg gggaauggug     3000

agaauugacg uuuuugccaa agauggcaag uacuacuucg ugcccaucua ugucgcggac     3060

gugcugaagc gggagcuucc uaaccgccug gcuacggccc auaaacccua cuccgaaugg     3120

agaguggucg augacagcua ucaauuuaag uucucccucu acccgaauga ugccguuaug     3180

auuaaaccuu cucgggaggu ggacaucaca uauaaggauc gcaaagaacc cguggggcug     3240

agacugaugu acuuugcugg cgccgacauc agcggagcua cccuuacacu gcggacccac     3300

gacaacuccg gcgagcucaa ggggcugggc auucagggac ucgaggucuu cgaaaaguau     3360

gugguugggc cccugggaga uacccauccu guguacaaag agagacgcau gccguuucgg     3420

gucgaaagaa agaugaauug a                                               3441


<210>  4016
<211>  3447
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4016
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccaucggc       60

uucgccgugg ucgaugcuaa cucucaccuu cuuagacuga aaggggagac agccauugga      120

gcgcggcucu uucgcgaagg ccaaagcgcu gccgacagac gggggucccg cacgaccaga      180

cggcgccugu cuagaacacg guggcgccuu agcuuccuga gagauuucuu cgccccucau      240

aucaccaaga uugacccgga cuuuuuccuc cggcagaaau auuccgagau cucucccaag      300

gauaaagauc gcuuuaagua cgaaaagaga cuguucaaug accggacaga ugcugaguuu      360

uaugaagccu acccuagcau guaucaucug cgccucagac ugaugaccga cgcuucccag      420

gccgaucccc gggagaucuu ccuugcgauu caccacaucc ugaaaagcag aggacauuuu      480

cucacaccug gcgccgcuaa ggacuucaac accgauaaag uugaccugga ggacauuuuu      540

cccgcccuua cggaagccua cgcccaggug uauccugauc uggagcucac cuucgaccug      600

gcgaaggccg augacuucaa agcuaagcug cucgaugaac aagccacacc gucugacacc      660

cagaaggcuc uggugaaucu ucugcucucc agcgauggcg agaaagagau cgucaagaaa      720

cgcaaacagg ugcugacaga auuugccaag gccauuaccg ggcuuaagac gaaauucaac      780

cuggcucucg gaacagaggu ugacgaagcg gaugccucua auuggcaguu uuccaugggc      840

caacuggacg auaaguggag caacaucgag accucuauga cagaccaggg gaccgaaauu      900

uucgagcaga uccaggagcu guaccgggcu agacuccuga auggaaucgu gcccgccggc      960

augucccuua gccaagccaa ggucgcugac uacgggcagc acaacgaaga ucuggagcuc     1020

uuuaaaaccu aucugaagaa acuuaaugac cacgaacugg ccaagacaau ucgcgugauc     1080

uacgaucggu auauuaacgg agacgaugcg aaaccuuucc ucagagagga cuuuguuaag     1140

gcucugacga aggaagugac cgcccauccc aaugaggucu ccgagcagcu gcucaaccgc     1200

augggccaag ccaauuucau gcugaaacag cggacaaagg cuaacggcgc caucccugug     1260

cagcuucagc aaagagaacu ggaucagauu aucgcuaacc agucuaaaua cuaugacugg     1320

cucgccaagc ccaauccggu ggagaaacac cgcgcgagca agccuuacca gcuggaugaa     1380

cugcuuaccu uucggauccc cuauuacguc gggccucuca uuacacccaa ggagcaagcc     1440

aaauccggag aaaacguuuu cgcuuggaug gugagaaagg accccagcgg caauaucacc     1500

ccuuacaacu uugaugagaa aguggaccgc gaggccucug cuaaugucuu cauucagcgg     1560

augaagacga cagauaccua ucugaucggg gaagacgugc ugccgaaaca gucccuccug     1620

uaccagaagu augagguucu uaacgaacug aauaacguga gagucaauaa cgaggcccuc     1680

ggaaccgacc aaaagcagcg gcugauucgc gagguguuug aaagacauag cucuguuaca     1740

aucaaacagg uggcggauaa ucuggucgcc cacggcgacu ucgcucagcg gcccgagauc     1800

agagggcuug ccgaugaaaa gcgcuuccuc agcucccugu cuaccuacca ccaacugaaa     1860

gagauucucc auggagcuau cgacgauagc acaaagcugc acgacauuga aaacaucauu     1920

acguggucca ccguguuuga ggaugccgcc aucuucaaaa caaagcuggc ugagauugac     1980

uggcuggaug cggccaccau caaaaagcuc agcaauaucc gguauagagg cuggggccag     2040

uuuucucgca agcugcugga cgggcuuaaa cucggaaacg gccagacagu cauucaagaa     2100

cugaugcugu ccaaucauaa ccucaugcag auccugaccg augagacgcu uaagaccaca     2160

auggcugaac ugaaccagga caaacucaag gccgaugaca uugaggacgu gaucaaugau     2220

gccuacacca gcccuucuaa caaaaaggcu cugagacagg uucugcgggu ggucgaagac     2280

auuaagcacg ccgcgaaugg gcaagauccc uccuggcuuu auaucgagac agcugacgga     2340

ccuggcaccg ccgggaaacg cacgcagagc agacagaagc agauccaaac aguguacgcc     2400

aacgcugccc aggagcucau ugauuccgcg guucggggcg aacuggagga caaaaucgcu     2460

gauaaggccu cuuucaccga ccgccuggug cucuacuuua ugcagggagg cagagauauu     2520

uauacagggg cuccccugaa uaucgaccag cuuagccauu acgauauuga ccacauccug     2580

ccgcaauccc ucaucaaaga cgauucucug gacaaccggg uccuggugaa ugcuaccauu     2640

aaccgcgaaa agaauaacgu guucaccagc acacuuuuug ccuccaagau ggcugccacg     2700

uggagaaaau ggcacgaggc gggacugauc agcggccgga agcuccgcaa ucugaugcuc     2760

agaccugaug aaauugacaa auucgcccau ggguuuguug cucggcagcu ggucgagacc     2820

agacagauca uuaaacuuac agagcaaauc gccgcugccc aguaucccaa caccaagauc     2880

auugccguga aggcuggacu gucucaccag cuccgcgagg aacuggauuu cccuaagaau     2940

cgggacguga accauuacca ccacgcguuu gaugccuucc uggcugccag aaucggcaca     3000

uaucuucuga aacgcuacgc caagcucgcu cccuuuuuca ccuaugggga auuugccaaa     3060

gucgacguga agaaauuucg ggaguucaau uuuauuggcg cgcugaagca ugcuaaaaag     3120

aacaucguug ccaaggauac gggagagauu gugugggaca aagaaagaga uauccaggag     3180

cucgaccgca uuuacaacuu caagcggaug cugaucacac acgaagucua cuuugagacc     3240

gccgaucuuu ucaaacaaac caucuaugcu gccaaggacu ccaaagaaag aggcgggagc     3300

aagcagcuga uuccuaagaa acgcggauac cccacacagg uguauggcgg gauccaugga     3360

aagauucggc ucaccuggag auggcugaaa cugcuucggc gcaagaaaau gucuaucgag     3420

cuguccgagc uccagcugcu caaguga                                         3447


<210>  4017
<211>  3450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4017
auggagagau accacaucgg ccuggacauu gggaccagcu ccaucggaug ggccgugauu       60

ggcgaugacu ucaagaucaa acggaagaaa gggaagaacc ucauuggagu ccgccuguuu      120

aaagaaggcg auacagcugc cgagagacgg ggguuccgca cgcaaagacg gcgccuuaau      180

agacggaagu ggcgccugaa gcuccuggaa gagaucuuug accccuauau ggcggaaguu      240

gauaaauacu ucuuugcuag acuuaaggag ucuaaccuga gcccuaaaga cuccaauaag      300

aaauaucucg gaucucugcu guucccggau aucagcgacu ccaacuuuua cgauaaguau      360

cccaccauuu accaucuccg gcgcgaccug auggagaagg auaaaaaguu cgaccuuaga      420

gaaaucuauc uggccauuca ccauaucgug aaauaccggg gcaauuuucu cgagaaggug      480

ccugccaaaa acuacaaaaa uucuggcgcu agcauugggu uccugcuuga agaggucaac      540

gaucuguaug gaaauaucau uggcaacgag gacguggcca uccucgacaa ugauaaauuu      600

gaagacguug agaagaucau ucugaacgau gaaauccgca auauugacaa gcaaaagaac      660

guggggagac ugcucgucaa ggauaaaaag gagaaaaaua ucgugacagc uuucuccaag      720

gccauuuuug gauacaaguu caaccuggaa gaccuucugc ucaucgagag cgaugagaaa      780

aacaagcuga ccuucaauga cgaaaacauc gaugacauuu uuaaugagcu uucucacucc      840

cugaacgaua aucagaugga ccuccugaca aaaacccggg aaaucuauuu caaguuuaaa      900

cugaacauga uuguucccac aggcuacacc aucagcgagu cuaugauuga aaaguaugag      960

augcauaagg cgcaccucaa aauguacaag gaguucauca auacgcugaa cgccaaagac     1020

agaaagaucc uuaaaaaugc uuauuccgau uacauuaaca augaaaaggc caaggcugcc     1080

aacgcgcagg agaauuuuua caaaaccgug aagaaaacaa ucaaggaaaa cgacagcgau     1140

accgccaaaa agauuaucgg gcugauugac gagggaaauu ucaugccuaa acagcgcaca     1200

ggcgaaaacg gcgucauccc ccaccaacuc caucagauug agcuggaucg gaucaucgag     1260

aaccaggcua aguauuaccc uuggcuugcc gaagagaauc cgguggaaaa gaacagaaaa     1320

uuugcuaagu auaaacugga cgagcucgug accuuccgcg uccccuacua uguugggccu     1380

cugauugaua agacggaguc uaauaaaaac gaaaaggaga caaaguuugc cuggauggug     1440

cggaaagcca agggaaccau cacacccugg aauuucgaaa accuggugga cagaaccgag     1500

uccgcuaauc gcuuuauuaa acggaugacc agcaaggaua cauacaucau uggcgaagac     1560

guccucccug cguccucucu gcuuuaugag aaauacaagg ugcugaacga gcucaauaac     1620

aucaagguua auaaaaagaa acuggaugug gaacagaagc aacacgucua ccuggaccuu     1680

uucacgaccc gcaaaaacgu gacaaaggau gaccucgcca ccagccugaa uugcgacguu     1740

gaguccauca cagggcugac cgauaacaag aaauuuaaua gcucucucuc cagcuauauu     1800

gaccugaagg cuauccuugg aaacauugug gaugacuacu cuaaaaacga agaucuggag     1860

aagaucauug aauauagcac gaucuucgag gacggcaaua ucuacaaaga gaagcucucc     1920

gaaauuucuu ggcugacaga ugagcagauc gaaaaacuga gcaacauuca uuucaagggg     1980

uggggaagac uuuccaagaa acuccugacc cagaucacca augagaacgg cgaacggauu     2040

aucgacacac uguggaauac cagcaacaau uuuauccagg ucauuucuga ugaguccauc     2100

caagccaagc ucgccgagau uaacggcgaa uaugcuaaua aauacaaccu ggaggacauc     2160

cuugaugaag ccuauacaag cccccagaau aagaaagcga uuagacaggu gaugaagguc     2220

guggaggaca ucgagaaggc uaugaaaugu gaaccgacgu cuauugccau cgaguucacc     2280

cgcgaaaagc ggaaauccaa gcugacaaac accagauaca aaaagaucag cgagacauac     2340

gaaaagauua ccgaugagcu caucuccgag uaugaacugg ggaaacugca aucugagcuu     2400

gacagcaagg uuaauaacau gcgggaccgc uacuaucucu acuuuaugca gcugggaaga     2460

gauauguaua cgggcgagaa aaugaauauu gacgaacugc accagaagua cgauaucgac     2520

cacauucucc cucaguccuu caucaaagau gacucucuga acaaccgggu gcuuaccagc     2580

aaguccguca auaucaagga gaaaagcgau aagacagccg cugaccugua ugccgcuaaa     2640

augggggauu uuuggagaaa gcuccgcaaa caaggacuga ugaccgaaca gaaguacaag     2700

aaccugcuua cacggaccga cucuauuaau aaauacacga agcaguccuu caucaaaaga     2760

cagcucgugg agacaagcca aguugugaag cuggccgcga acauucugca ggauaaauau     2820

cgcaauacca agaucauuga aaucagagcc cggcucaacu cugaccugcg caaaaaguac     2880

gagcuuauca agaauagaga agucaacgac uaucaucacg cuauugaugg cuaccugaca     2940

accuuugugg ggcaguaucu cuacaaagug uaucccaagc ugcgguccua cuucguuuac     3000

gacgauuuua aaaagcugga cagcaauuau cuuaaacaua uggauaaguu caacuuuauc     3060

uggaagcugg aggacaaaaa ggccgaagau gucuacgaca aagugaauga ugaguucgug     3120

cucaacgucc cugagaugaa ggaauauauu cgcaaaaucu acaauuauaa guacaugcug     3180

gugucuaagg agguuaccac gaaaaacggc gcuuuuuaug accagacaaa guacaaugcc     3240

aaaaccauua accucauccc cauuaagaaa gauaagccca caaauaucua cggaggcuau     3300

aaggggaaag ugagcuccua caugaugcug gucaagaucc agaaaaagaa agaagugauu     3360

uauaaguucg uuggagugcc uagacuuugg accgacgagc uggaucggcu caucgacaca     3420

gacgagaaaa aggcgcugcu gaagaaauga                                      3450


<210>  4018
<211>  3459
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4018
augaacgccg agcacggcaa ggaagggcug cucaucaugg aggaaaauuu ccaguacaga       60

auuggacugg acaucggcau uaccagcgug gggugggcug uccuucaaaa caauucccag      120

gaugagcccg uucggaucac agaccuggga gugcgcauuu uugauguggc cgaaaacccu      180

aaaaauggcg acgcgcucgc ugccccgaga cgggaugccc gcacgaccag acggcgccug      240

agacggcgca gacaucggcu ugagcgcauc aaguuccugc uccaggagaa cgggcugauc      300

gaaauggacu cuuuuaugga gagauauuac aaaggaaauc ugcccgaugu cuaucagcuc      360

cgguacgaag gccuggaccg caagcugaaa gaugaggagc uggcucaagu gcucauucac      420

aucgccaagc auagaggcuu ccggagcaca agaaaggcug aaaccaaaga gaaggaaggg      480

ggagccguuc ugaaagcgac aaccgagaac cagaagauua ugcaggaaaa aggcuaucgc      540

acaguggggg agaugcuuua ccuggacgag gccuuucaca ccgaaugccu cuggaaugag      600

aagggauaug uccugacgcc ucggaacaga cccgaugacu acaagcauac cauccugcgc      660

uccaugcucg uggaagaggu ucacgcuauu uucgccgcuc agcgggccca cggcaaucaa      720

aaagcgacag aagggcugga ggaggccuac guggaaauca ugaccucuca gcgcagcuuu      780

gauaugggac cuggccuuca gcccgacggc aagccuuccc cguaugcuau ggagggguuc      840

ggagacagag ucggcaaaug uacauuugaa aaggaugagu accgggcccc caaagcuacc      900

uauacggccg aacuguucgu ggcccuccag aagauuaacc auacaaaacu gaucgacgag      960

uuugggaccg gaagauucuu uucugaggaa gaacgcaaga caaucauugg ccuucugcuc     1020

agcuccaagg aacugaaaua cgggaccauc cggaagaaac ugaauauuga uccuagccuc     1080

aaguucaacu cucugaauua uuccgcuaaa aaggagggag agaccgaaga ggaaagagug     1140

cuugacacag agaaggcgaa auucgccagc auguuuugga cguacgaaua uucuaagugc     1200

cugaaagauc gcaccgagga gaugcccguc ggcgaaaagg cugaccucuu cgaucggauc     1260

ggcgagauuc ugacagccua caaaaacgac gauuccagaa gcucucggcu uaaggaacug     1320

gggcucuccg gagaggaaau cgacggccug cuggaucuca gcccugccaa guaccaacgc     1380

guuucccuga aagcuaugag aaagaugcag cccuaucuug aggacgggcu gaucuacgac     1440

aaagccugug aggcggcugg auaugauuuu cgggcccuca acgacggcaa uaagaaacac     1500

cuucugaagg gggaagagau uaacgccauc gugaaugaua uuaccaaccc gguggucaag     1560

agaucuguga gccagacaau caaaguuauu aaugcuauca uccaaaagua cggcuccccu     1620

caggccguga acauugaacu ugcucgcgag augagcaaaa auuuccagga ccggaccaac     1680

cucgaaaagg agaugaaaaa gagacagcaa gagaaugaac gcgccaaaca gcagaucauu     1740

gagcugggaa agcagaaccc cacgggccaa gauauccuga aguauagacu cuggaaugac     1800

caagggggau acugccugua uucuggcaaa aagauuccuc uugaggagcu guuugauggg     1860

ggauacgaca ucgaucauau ucuccccuac uccaucacau ucgacgauag cuaucggaac     1920

aaaguccugg ugaccgcgca ggagaaucgc cagaagggca acagaacccc cuacgaauau     1980

uuuggggccg acgagaaacg gugggaagau uacgaggcuu cuguucgccu gcuugugaga     2040

gacuauaaga agcagcagaa acuccugaag aaaaauuuca cagaagagga gcggaaggaa     2100

uuuaaagagc gcaaccugaa cgacaccaag uacaucacaa gagucgugua uaauaugauu     2160

cggcagaacc ucgaacugga gccuuucaau cacccggaaa agaaaaagca ggucugggcc     2220

gugaacggcg cuguuacgag cuaccuucgc aaaagauggg gacugaugca aaaggaucgg     2280

uccaccgaca gacaccaugc cauggaugcg guggucaucg ccuguugcac agacggcaug     2340

auucacaaaa ucucucgcua caugcagggg cgggagcucg cuuauagcag aaauuuuaag     2400

uuccccgaug aggaaaccgg agagauucug aaccgcgaca auuuuacacg ggaacagugg     2460

gaugagaagu ucggcgugaa aguuccucug cccuggaacu ccuucagaga cgaacuugau     2520

auccgccucc ugaaugagga cccuaagaac uuucugcuca cccaugccga ugugcaacgg     2580

gagcuggacu accccgggug gauguaugga gaagaggaaa gcccgaucga ggagggcaga     2640

uacauuaauu auauccgccc ucuuuucguc ucucggaugc ccaaccacaa agugacgggg     2700

uccgcucaug augccaccau uagaagcgcc cgggacuacg aaacacgcgg cgugguuauc     2760

accaaggucc cucugacaga ccucaaacug aauaaggaua acgagauuga aggauauuac     2820

gacaaagauu cugacagacu gcuuuaccag gcucucgugc ggcagcugcu gcuccacggc     2880

aaugauggga agaaggcguu ugccgaggac uuccacaaac ccaaggcuga uggaaccgaa     2940

ggcccugugg uccgcaaagu gaagaucgag aaaaagcaga cguccggggu uauggugaga     3000

ggaggcacag ggaucgccgc caacggcgag augguccgga uugacguguu uagagaaaac     3060

ggaaaguauu acuucguucc cguguauacc gcugaugucg ugcgcaaagu ucugccgaau     3120

cgggccgcga cacauaccaa gccuuacagc gaguggagag ugauggacga ugcuaacuuu     3180

gucuucuccc uuuauucucg cgaccugauc cacgugaaaa gcaagaaaga cauuaagacc     3240

aaucucguga acggcgggcu gcugcuucaa aaggaaaucu uugccuacua uacaggagcc     3300

gauauugcua cggccuccau cgcgggcuuc gcuaaugacu cuaacuuuaa auuccggggg     3360

cugggaaucc agagccucga gauuuuugaa aagugucagg ucgauauccu gggcaauauu     3420

uccguuguga gacaugagaa ccgccaggag uuccacuga                            3459


<210>  4019
<211>  3462
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4019
augaagaaca uccugggccu cgaccugggg cuuagcucca uuggaugguc ugugaucaga       60

gagaauagcg aagaacagga acuggucgcc augggcuccc ggguuguguc ucucaccgcu      120

gccgagcuga gcuccuucac acaagggaac ggagugucua uuaauagcca gcgcacgcag      180

aaaagaaccc agcggaaggg cuacgaucgc uaucaacuua gacggacacu gcuccgcaac      240

aaacuggaca cccuggggau gcuccccgau gacucccuga gcuaccuucc uaagcugcag      300

cucuggggac ugagagcgaa agcugucaca cagcggaucg aacuuaauga gcugggccgc      360

gugcuccugc accugaacca gaagagaggc uauaagucua uuaaauccga uuuuagcggg      420

gacaagaaaa ucaccgauua cguuaagaca gugaaaaccc gguaugacga gcucaaggaa      480

augcgccuga cgaucggaga gcuuuucuuu agacggcuga ccgaaaaugc cuucuuucgc      540

ugcaaggagc aagucuaccc gagacaggcc uauguggagg aguucgauug uauuaugaac      600

ugccagcgga aauuuuaccc cgacauccuc acagaugaga ccauuagaug uauccgcgac      660

gaaauuaucu acuaucagcg gccucugaag ucuugcaaau accuuguuuc cagaugugag      720

uucgaaaagc gcuuuuaucu gaaugcugcc ggcaaaaaga cagaggcugg gcccaaagug      780

agcccucgga ccucuccccu cuuccaaguc ugcagacugu gggaguccau uaacaauauc      840

guggugaagg aucgccggaa cgaaaucguc uuuauuagcg ccgagcagag agcggcccug      900

uucgacuuuc ucaauacgca ugaaaagcug aaaggauccg accuucugaa gcuccugggc      960

cuuucuaaaa cauacgggua ucgccuggga gagcaguuca agaccggcau ccaaggcaac     1020

aaaacacggg uugaaauuga gagagcucuc gggaauuacc cugauaagaa gcgccugcug     1080

caguucaacc uccaggagga aagcuccagc auggugaaca ccgagaccgg agaaaucauu     1140

ccgaugaucu cucuguccuu ugagcaggaa ccccuuuauc ggcuguggca cgugcucuac     1200

agcaucgacg auagagagca acugcagucu guccugcggc agaaauucgg cauugacgau     1260

gacgaggugc uugaacgccu cagcgccauc gaucugguua aggcuggguu uggaaauaaa     1320

uccucuaagg ccauuagacg gauccugccu uuccuccagc ugggcaugaa cuacgcggag     1380

gccugugaag cugccgggua uaaucauagc aacaauuaca caaaagcuga gaacgaggcc     1440

agagcccuuc uggaccgccu ccccgcuauu aagaagaaug aacugcggca accugugguc     1500

gagaaaaucc ugaaccagau ggugaauguu gugaacgcgc uuauggaaaa guauggaaga     1560

uuugaugaga uccgcgucga acucgccaga gagcugaaac aguccaagga ggaacggagc     1620

aauacguaca aaucuauuaa caagaaucag cgcgagaacg aacaaaucgc uaagagaauu     1680

guggaguaug gcguccccac ccggucccgc auccagaaau acaagaugug ggaagagagc     1740

aaacacugcu guauuuauug cggccagccg guggacguug gggauuuccu gagaggauuu     1800

gacguggagg ucgaacauau cauuccuaag ucucucuacu ucgacgauuc cuuugccaau     1860

aaagugugua gcugccgguc cugcaacaag gagaaaaaca aucgcacagc cuacgacuau     1920

augaagucua agggcgaaaa agcucugagc gauuacguug agagagugaa caccauguau     1980

acaaauaacc agaucuccaa gaccaaaugg caaaaucuuc ugacgcccgu cgacaagauc     2040

ucuauugauu ucaucgaccg gcagcuccgc gaaagccagu acauugccag aaaagcgaag     2100

gagauccuga cauccauuug uuauaacgug accgcuacca gcgggucugu gacauccuuu     2160

cugcggcacg uuuggggaug ggauaccguc cuucacgacc ucaauuucga uagauacaag     2220

aaagugggcc ugacagaggu gaucgaaguc aaccaucgcg ggagcgugau ccggagagag     2280

caaauuaagg acuggucuaa acgcuuugau caccggcauc acgccaucga cgcccugacg     2340

auugcuugca ccaagcaggc cuauauccag agacucaaua accugcgcgc ugaagagggc     2400

ccugauuuca auaaaauguc ccuugagcgg uacauucaga gccaacccca cuucucuguu     2460

gcccagguga gagaagcggu cgaccgcauc cuggugagcu uucgggccgg aaagagagcu     2520

guuacacccg gcaagcggua cauccgcaaa aacagaaagc ggauuuccgu gcagagcguc     2580

cucaucccuc gcggggcccu gucugaggaa uccguguaug gaguuauuca ugugugggag     2640

aaagacgaac agggccacgu cauccaaaag cagagagcug ugaugaaaua cccgauuacc     2700

agcaucaauc gggagaugcu ggauaaggag aagguggucg acaaaagaau ccaucgcauu     2760

cuuuccgggc ggcucgccca guauaacgau aaucccaagg aagcguucgc caaaccuguu     2820

uacaucgaca aggagugucg cauucccauc agaacagugc ggugcuuugc uaaaccugcc     2880

auuaacaccc uggugccccu gaagaaagau gacaagggaa acccggucgc uugggugaau     2940

ccuggcaaca aucaccaugu ugccaucuau agagaugaag acgggaagua caaagagcgc     3000

acggugaccu ucugggaagc cgucgaucgg uguagagugg gcauucccgc uaucguuaca     3060

cagccugaca ccaucuggga uaacauucuc caacgcaaug acaucucuga gaacgugcug     3120

gagagccuuc ccgaugucaa guggcaguuu gugcuguccc uccagcaaaa ugaaauguuc     3180

auucugggaa ugaacgagga agacuauaga uacgcgaugg accagcagga uuacgcccug     3240

cuuaauaaau aucuguaccg gguucagaag cucucuaaaa gcgacuauuc cuuucgcuac     3300

cacacagaga ccucugugga agauaaguau gacggcaagc cuaaccugaa acucagcaug     3360

caaaugggga agcugaaaag agucuccauc aagagccuuc ugggacucaa uccccacaaa     3420

gugcauauuu cugugcuggg cgagaucaag gagaucuccu ga                        3462


<210>  4020
<211>  3474
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4020
augaacuacu auaauggcuu caucauuacc gagaacgcca ugacaagcaa gcaggaaaaa       60

cacggggcuc ugacguuugg acucgacauc ggcauugccu ccguggggug ggcgguccug      120

ggagagucua gaaucguuga ucuuggcgug cggugcuucg acaaggcuga aaccgccaaa      180

gagggggaaa gccugaaucu cacacgcaga auggcccggc ugcuucgcag acggcugcgc      240

agacgggcuu ggcgccucac caagcuggcc agacugcuca aacgggaggg acugauugcu      300

gaugccaacc uuuuuaagca uaagaucgag aaaaauacac ccgugccuaa guccucuugg      360

caacugcgcg ucgacggccu cgauagacag cugaacgcgg aagagugggc ccgggugauc      420

uaccaccuuu guaaacaucg cggcuuccac uggauuagca gagcugaaga gaaggccgcu      480

gagggggacu ccaaaggaga aggcgggaag guuaagcagg gacuggccgg caccaaaaag      540

cucaugcagg agaaaucuua ucggacagcg gccgaaaugg ugcuggcuga guuuccggau      600

gcccaaagaa auaagcaggg ggacuacagc aaagcucugu cccgcauccu ccugggagau      660

gaacuuaagg cccuguucga gcaucagcgg aaacucggca accccgucgc caccgagaag      720

cuggaagcug cgauucuugg caauggggac aagaaaugcg gacuguuuug ggcccagaac      780

ccugcucucg ccggcaauaa gcugcuggag augcucggga gauguacguu cgagaaaacc      840

ggaggcgaug acgggaaggg agaguaucgc gcccccaaag cuagcuuuuc ugccgaacgg      900

cacguguggc ugacacaccu uaacaaucug agaaucguug uggauggcac cacacgcccu      960

cucaacgagg cggagcggag agucgcucug cccaugccuu accaacaggc cggcgaccuu     1020

uccuauaagc agcucggggg agcccucauu aaggaaggcu uccugcagaa agacggguuu     1080

aaguucgcug gacugccgua ccccaccaaa caagccagcg aggaaaaggc uaaagauccu     1140

gagacgucug ugcucugcaa gcugcccgcc uggcauacac uucgcaagac ccuggaaaaa     1200

cacggccucg aguccgagug gcagaagcug agcacagacg cgcuggaugg gaaaccucag     1260

cuucucgaca auaucgccug ggugcugucu gucuacaagg augacgauga aguuaaaacc     1320

gagcugggca agcucaaccu gcccaacacc gaaaagauga uugacgcccu ucuggauauc     1380

cgguuugaca aauucuccaa ucucagccug aaggcucugu ccaaaaucgu gccguucaug     1440

gagcaaggac agagauauga ugaggcugug gccaacauuc cugaauacgg ccaucacucu     1500

cagcuucaca aggucggggc gggagagcau aaauaucugc ccccuuuuua caaggaacgc     1560

gacaaaacag gccggauggu guucaaugac gauauggaca uccccagaaa ccccguugug     1620

cugcgggccc ugaaucaggc ucgcaagguc gugaacgccc ucauuaagga guaugggagc     1680

ccucacgaag uucauaucga gauggcuaga gaucuguccc ggccgcuuga cgagagacgc     1740

gauauugaaa aacugcaaaa ggaguuugga gaacggaaua aaaaggacaa agaacucuuc     1800

gcccaggauu uuaacaucgu gggcacgguc aaggggaagc aguucgaaaa auaccagcug     1860

uauagagagc aacagggcaa guguaucuac cccagcaugg acgagaaaca gggaauuugc     1920

uguuacucuc ugaagccucu ugaucuccac cgcgugcugu augaagaggg cuacgccgaa     1980

aucgaccaug cucugcccua uucccggagc uuugaugacu cuaaaaauaa ccggguccuc     2040

gugcuggcga aggagaaucg cgauaagggg aacagaaccc cuuucgaaua ccuuggagac     2100

aaagacaaua gcgagcccug gcggcaguuu guugccuucg uggagggcaa caaggcuuau     2160

cgccuggcca aaagaucccg gcuccugcgc aaggauuuug gggacaaaga agccaaggag     2220

uucagagaac ggaaucugaa cgauacacgc uacauuugca aguuuuucaa aaacuauguc     2280

gagcaauacc uucagcucca cgaggacucu gaagcuaaga gauguguggu ucugagcgga     2340

cagaugaccu ccuuccugcg ggccagaugg ggccucguga aaguccgcgg ggauagcgac     2400

cggcaccaug cgcuggaugc ugccguggug gccgcuugcu cucacggcau gguuaagaga     2460

cuuuccgacu acagccgccg gaaagagcug gaacagguca gagagggauu uguggaugug     2520

gacacaggcg aaaucgucaa uccggccaug uuccaacagg ugcaggagca uuuuccuaac     2580

cccuggccuc acuuccgcga ugagguuaag cuccggcugu cuauugacga ucccacccug     2640

cuuagauccg aaauggagaa auuugggacg uauacaccug aaagccugga ggcgcuccgc     2700

ccccuguucg ugucccgggc uccgcaaaga agaaauucug gagccgccca caaggacacc     2760

aucuacgcuc agccuaagcg ccucgucgcc cagggcgggg ugacccagaa aguugcucug     2820

agcucccuua cacugaagga ccucgauaaa cugaucgacc cccauagaaa cgaacggcug     2880

uaugccgcga uucgcgccag acuugagcaa cacggaggca agggggauaa ggcuuuuccc     2940

gccucuaauc cucugcggaa accggacagc gagggcaacc ccaccggacc uaucgugaga     3000

ggcgucacaa uggugauuga uaagcucucc gggaucccca uucgcggagg caucgcuaaa     3060

aaugacagca ugguucgggu ggaugucuuc acgaaggcca acaaauuuca ucuggugccu     3120

guguacgucc accauucugu ucucgggcug cccaaucagg ccauuguggg aggcaaggaa     3180

gaguccgaau ggacccagau cgacgauagc uucgacuggc acuuuucuau cuauccuaac     3240

gaucuuauua gagugacacu gaaacaggag ggccgcuucg gguacuaugc uggaugugac     3300

cgguccaccg cggccaucca ccucugggcu caugacagaa aucaacaggu cggcaaggau     3360

gggcugauuc gcagcaucgg agugaagaca gcccugucua uugaaaaauu uaacguugac     3420

gugcuuggca aucgguaccc cgccccgccu gagaagagac gcgaucuggc uuga           3474


<210>  4021
<211>  3486
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4021
augaagaaaa uccugggccu cgaccugggg agcgccucca uuggaugggc ucuuguggag       60

gaaaccgagc agaugcaaua caacaucguc ucucugggca gcagaauuau ccccuauaau      120

gggacagaag gacaggaguu cgaaaagggc acgggggagu cccggaacuc ucuccgcacc      180

aaagccagaa cagcgcggaa gggauacgau cgcuaucagc ugagacggaa aaaucuugag      240

aagauucugc uccagaacgg cauguacccu aaugaacaag uucgcgcucu gcagaagcug      300

gagcucugga aacugagagc cgacgccccg aacgugcaga ucagccuucc cgaacugggc      360

cggcuccugu uuuggcuuaa ucagaagcgc ggguauaaau ccucuagaag cgaugcuaac      420

cuggacaaga aagauaccga guacguggcc acagucaagu cccggcacca aaagaucaaa      480

gacgcuggac agaccguggg ccaguauuuc uacgggcaga uucucgagaa ugaauaccau      540

cgcaucaagg agcaaguuuu uccuagagaa gccuauauug aggaguucga ugcgaucgug      600

gcccggcaga aagaauacua ucccacaauu cugaagcagg agcugaucga caaaauucgc      660

aacgaaguca ucuacuuuca aagaggacuc aagagccaga aaggccuggu gucuguuugc      720

gaguucgaag ggcgggaguu uaauggacac uucggcggcc cuaagguggc ucccaagucc      780

agcccuaucu uucagcuugg gaaaauuugg gaaaacauca auaacauuaa gaucgccgau      840

auugagcuga ccgcugagca gaaacucguc uucuuuaauu aucugaacug ucaugaaaga      900

aucacggagg ccaagauccg cgaacuucug aaacucucua aggacucccg gcugaacgga      960

caacugaaga auggccucac cgggaacaaa acauacagcc agauugcguc uugcuucgga     1020

aauaagaucg gcgaguauga aagacuguuu aaauucgagc uugaggugau ugaauccgag     1080

aagcuggaau acaccuacca cccgaaaagc cauauccccg ugggggagaa gcgccggaag     1140

uauauuuccg agaacgucga aaaagagccu uacuauagac ucuggcacac aaucuacucu     1200

aucaaggaua aagacgaaug ucgcgccgcu cugauuaaga auuucggaau cgaggaagag     1260

auugccgaga aacuugcuag ccuggauuuu uccgccggcg gcuucgggaa caagugcgcc     1320

aagguuaucc ggaaaauucu ccccuaucug auggaaggag acaaguacaa uguggcuaug     1380

gacuaugcgg gcuacaacca cagcuuuucu cagaccaaag augagaaucu gcagagagug     1440

cuccuggacu ccaucaacca ucuuccuaag aauagccugc gccaacccau ugucgaaaag     1500

auccucaacc agaugaucaa ucuggugaac gagguuauua agcagugggg gcggccggau     1560

gaaaucagag uggagcuggc ccgcaaacuu aagcagucua agacggagcg gaaugacgau     1620

gacaacuuca ugagaaaacg ggaacgcgag aacgaaauua ucaaggcuga ucucgccaaa     1680

uacggacuga aggccacaag aaauaacguc auuaaauggc ggcuguauca cgagaucgac     1740

gauaaggaca agaaacucaa ugcuagaugu aucuacugcg gccaaccuau uagccugacc     1800

gaagccaucc uugggaacaa uguggauguu gagcauauua uccccaagga caaacuguuu     1860

gacgauuccc agucuaacaa gacacucgcg caccgcagcu guaauuccgg caaaggaaac     1920

aagaccgcuu augacuucau gaaggauaaa ccucaguuug aggaauacgu ggcccggguc     1980

gaggaacugu auaagaaaca ccugauuagc cuuaccaaga aaagaaaguu ccucaugucu     2040

gaggacaaga uccccuccga uuuuaucgac cgccagcuga gagagagcca auacauugcc     2100

cggaaaucuc gcgaaauccu gcagacagug ugcagaaaug ucugguccac ggauggcggg     2160

gugaccgcuc aucuccggaa gcugugggga ugggacgaug uuacaaugaa ccuucagaug     2220

cccaaauaua agggcauugg gaugaccgag aucguggaau gggagagcaa ucacggcaaa     2280

aaccaacaua cacaggaagu cauuaaggac ugguccaaac gcgaugacca cagacaucac     2340

gccaucgaug cucugguggu ugccuguacc cggcagggau acauccagcg cauuaauacg     2400

cucaacucua gcgagacaaa ggacgagaug cacagaaagg uggcgggguc ccuggucgag     2460

uucaaucaaa aacauacccu gcuuaacaag uaccucauca ccgaaaaacc uuuuacaacc     2520

ucugaagugg aggacguggu uagcaagauu cuggucuccu ucaaagccgg caagaaggcu     2580

gccacaccgg gaaaacggaa ggugggcaaa cgcgggaaca aacagguggu ccaggaugga     2640

aucauuaucc ccagaggcgc ucugcacgcc gacagcgugu cuggguccau uaaacaguau     2700

gauccuacgg gaaauuuuga aaaccauaau guuaucaagu acaagguggg cgucgggaaa     2760

ggcuaucucu ucgacggaaa ggaugacaaa aagaaaaucg auaagaccau ugacugcauc     2820

guggauaaga aaauucggga ccugaucaag caaagagcgg aggagagcaa cuacucucuu     2880

aaaaagaugu ucgaaacacc cauucuguuu aaucagcgcg ccguuuucuc cgugcggugu     2940

aaaaccggca acaaggcugu cgugccuauc agaaaugggu auguugugcc cgauaacaau     3000

caccacacag ccaucuacaa guaugacgga ggccucaaag agcagugcgu cagccugaug     3060

caggcuauug aacgcaagcg guacaaccug ccgaucauua ucaaaaaucc uaaggagauu     3120

uacgauaaaa ucaccgaaau cgccaacauu aaucaacuuc ucuuugacuc ucugcccaag     3180

cuggacuggg aguucgugac gacccuccag aaaaacgaua uguuuauccu ggggauggac     3240

uauagcgcca cagaggaagc ucuugcggau ggaaauuaca gacugcucuc cgaguaucug     3300

uacaaggugc agaacauuag cgacggcauc uaucgccugu gccaucacac cauuacaaag     3360

uucgaucuuu cuaaagccaa caagccugac aaacgguuuu acaauaucca aucccugaag     3420

gcuuggaaca cccucaaucc ccagaaaguc aagauuaaca uccuggggca gaucgaaaga     3480

caguga                                                                3486


<210>  4022
<211>  3501
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4022
augcacgugg agaucgacuu cccccauuuu agcagaggcg auucccaccu ggccaugaac       60

aagaaugaaa uucuccgggg gucuagcguc cuguaccgcc uuggacugga ccucggcucc      120

aacucucugg ggugguucgu uacccaucuu gagaaaagag gagaucggca cgaaccugug      180

gcucugggcc ccgggggagu gcgcaucuuu cccgacggca gagauccuca gagcggcaca      240

uccaaugccg ucgaccggcg cauggcgaga ggggcucgga agcgcagaga ucgguucgug      300

gagcgcagaa aagaacucau ugccgcccug aucaaguaua accugcuccc cgacgaugcu      360

cgggagcgca gagcccugga gguucuugac ccuuacgcuc ugcggaaaac ggcccucacc      420

gauacacugc ccgcgcauca cgugggcaga gcccuuuuuc accugaauca acgccggggc      480

uuccagucua acagaaagac cgacagcaag caguccgaag auggggcuau uaaacaggcc      540

gcuagccgcc ucgccacaga caagggaaau gagacccugg gcgucuuuuu cgcggacaug      600

caucugcgga aaucuuauga agauagacaa acagccaucc gcgcugagcu cgugcggcug      660

gggaaggacc accuuaccgg aaacgccaga aagaagaucu gggcuaaggu ucgcaaacgg      720

cuguuuggcg augaggugcu cccuagagcc gacgccccgc auggcguccg cgcucgggcg      780

acgauuaccg ggacaaaggc cuccuacgau uauuacccca ccagagacau gcugcgggau      840

gaguucaaug cuaucugggc cggacagagc gcccaccacg cuacaauuac cgacgaagcc      900

cgcacggaaa ucgagcauau uaucuuuuau cagagaccuc uuaaacccgc gauugugggc      960

aagugcacac uggauccugc uacccggccc uucaaagaag acccggaggg guacagagcc     1020

ccuuggucuc acccccucgc ccagcgcuuu cggauccugu ccgaggcuag aaaccuggaa     1080

auccgcgaua caggaaaggg cagcagacgg cucaccaaag agcaaucuga ccuggugguc     1140

gccgcccuuc uggcuaaucg cgaaguuaag uucgacaagc ucagaacccu gcuuaaacug     1200

ccugcggagg cccgguucaa ccucgaaucc gaucgcagag cugcccugga cggggaucag     1260

acagcugccc ggcugagcga caagaaagga uuuaauaagg cguggcgcgg cuucccgccc     1320

gagagacaga uugccaucgu ggcucggcuc gaggaaacgg aggaugaaaa cgagcugauu     1380

gccuggcuug aaaaagagug ugcucuggac ggcgccgccg cugcgcgcgu ggccaauacc     1440

acacucccug augggcauug cagacuggga cugcgggcua ucaagaagau ugucccgauc     1500

augcaggacg gccuugauga ggacggggug gccggagccg gcuaccacau cgcugccaaa     1560

agagcggggu augaucaugc uaagcucccc accggcgaac aacugggacg ccugccuuac     1620

uauggccagu ggcuccagga cgccguugug ggguccggag augcccggga ccaaaaagag     1680

aagcaguacg gccaguuucc caacccuaca guccacauug ggcugggaca gcuuagacgc     1740

gugguuaacg accugaucga uaaauaugga cccccgaccg aaauuucuau cgaguucacg     1800

cgggcucuca agcugagcga gcaacagaag gccgaaagac agcgcgagca gcggagaaau     1860

caagacaaaa acaaggcgcg cgcugaggaa cuggccaaau uugggcggcc ugccaauccc     1920

agaaaccuuc ucaagaugcg gcugugggag gaacuggcuc acgauccucu cgaccgcaaa     1980

uguguguaca caggcgagca gauuuccauc gaaagacugc uuagcgauga ggucgacauc     2040

gaucauauuc ugcccguggc caugacccuc gacgauucuc cugcuaauaa gaucauuugc     2100

augcgguaug ccaaccgcca caagagaaaa cagacccccu ccgaagcguu cggaagcucu     2160

ccgacacugc agggccaucg guacaauugg gacgauaucg ccgcuagagc caccgggcug     2220

ccucgcaaca agcgguggag auuugacgcu aaugcccgcg aggaguucga uaaacgggga     2280

ggcuuucuug ccagacaacu caacgaaaca ggguggcugg cucgccuggc gaagcaguac     2340

cucggagccg ucacggaccc caaucagauu ugggugguuc ccggccggcu gaccagcaug     2400

cuuagaggga aauggggccu gaacggacuc cugccuuccg acaauuaugc uggcgugcag     2460

gauaaggccg aggaguuccu ggccucuaca gacgauaugg aauuuagcgg ggucaaaaac     2520

cgcgcugacc acagacacca ugccaucgau ggacuuguga ccgcgcucac agaccggucc     2580

cugcugugga agauggcuaa ugccuacgau gaggaacacg aaaaguucgu uauugagccg     2640

cccuggccua ccaugcgcga cgaucucaaa gccgcucugg agaagauggu ggucagccau     2700

aaacccgacc acggcaucga agggaaacuu caugaggauu cugccuaugg auuugugaaa     2760

ccucuggacg cgacgggccu caaggaagag gaggcuggga accuggugua cagaaaggcc     2820

aucgaauccc ugaacgagaa ugaaguugac cggauucgcg auauccaacu uagaaccauu     2880

guccgggacc acgugaacgu ggagaaaaca aagggcgucg cccuggcuga ugcccucaga     2940

cagcugcagg cucccagcga cgauuauccu caauucaaac acggacuccg ccaugugcgg     3000

auccugaaga aagaaaaggg cgacuaccuu guucccauug ccaauagagc gucuggggug     3060

gccuauaagg cuuacuccgc cggagagaac uucugugucg agguguuuga aaccgcuggc     3120

gggaaauggg auggagaggc cguucgccgg uucgacgcca auaagaaaaa cgcuggcccg     3180

aagaucgcgc acgccccuca guggagagau gcuaaugaag gcgccaaacu ggugaugcgg     3240

auccauaagg gggaccucau ucgccuggau cacgagggca gagcccggau cauggucgug     3300

caucgccugg acgcugccgc gggaagauuu aaacuugcug aucacaacga aacagggaau     3360

cuggacaagc ggcacgccac caacaaugac auugaucccu uccgcuggcu cauggccagc     3420

uacaacacgc ugaagaaacu cgcugccguu ccugugagag ucgacgagcu gggacgggug     3480

uggcgcguga ugcccaauug a                                               3501


<210>  4023
<211>  3507
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4023
auggccagac ccgcuuuccg ggccccucgc agagagcacg ugaacggcug gaccccggac       60

ccccaucgga ucagcaagcc cuuuuucauu cuggucuccu ggcaccuccu gucucgcguu      120

gugaucgaua gcuccucugg gugcuuuccc ggaacaagca gagaccauac ggauaaauuc      180

gcggaauggg agugugcugu gcagccuuac cggcuuuccu uugaccuggg caccaauucu      240

auuggguggg gacuccugaa ccuugaucgc caaggcaagc ccagagaaau ccgggcccug      300

gggagccgca uuuucuccga cggaagagau ccucaggaca aagccagccu cgcugucgcc      360

cggcgccugg cuagacagau gcggcgcaga cgggauagau aucugacacg ccggaccaga      420

cucaugggcg cccuggugcg cuuuggccuu augccggcgg accccgccgc ucggaagaga      480

cuggagguug ccguggaucc uuaccucgcu cgcgaacggg ccacaagaga gcgccuggag      540

cccuucgaaa ucgggcgggc gcuuuuucac cugaaucaga gacgcggaua uaaaccuguc      600

cggaccgcca caaagcccga cgaggaagcu ggcaagguga aagaggccgu ugagagacuc      660

gaagcugcca ucgccgcugc gggggccccg acccugggag cuugguucgc cuggcggaag      720

acgcgcggcg agacccugag agcccggcuc gcugggaaag gaaaggaagc cgcguacccu      780

uuuuaucccg cuagacgcau gcuggaggcc gaguucgaua cacuuugggc cgagcaagcu      840

cggcaucacc cugaccugcu caccgccgag gcuagagaaa uucugcgcca cagaaucuuu      900

caucagcggc cccuuaaacc cccuccggug ggccgcugca cacuguaccc cgacgauggc      960

agagccccuc gggcgcuccc cucugcccag cgccugagac uguuccagga gcucgcuucc     1020

cugcggguca uucaccuuga ccugagcgaa cgcccucuca cccccgccga gagagaucgg     1080

aucguggcuu ucgugcaagg gcgcccgccu aaggccggaa gaaagcccgg caaaguccag     1140

aagucuguuc cuuuugaaaa acugcggggg cuucuggagc ucccuccugg aacgggcuuc     1200

ucccuggaga gcgacaagag acccgaacug cucggggaug agacaggagc gcgcauugcc     1260

ccggcuuuug gcccuggcug gaccgcccug ccccuugaag aacaggacgc ucugguggaa     1320

cuccugcuga cagaggccga gcccgaacgg gccaucgcug cgcuuaccgc cagaugggcu     1380

cucgaugagg ccaccgccgc uaaacuggcc ggggcgacac ugccugacuu ccauggacgc     1440

uauggccgga gagcuguggc cgaacuccug ccgguccuug agcgcgagac gagaggggau     1500

cccgacggac gggugcgccc uauucggcug gaugaagccg uuaagcuccu gagaggcggg     1560

aaagaccacu cugacuuuuc ccgggagggc gcucugcuug augcccuccc cuacuacgga     1620

gcggugcugg aacgccacgu cgcuuucggc accgggaacc cugccgaccc cgaggaaaag     1680

agagugggac ggguugccaa uccuacagug cauaucgcuc ugaaccagcu ccgccaccug     1740

gucaaugcca uccuugcuag acauggccgg cccgaggaga uugugaucga acuggccaga     1800

gaucucaagc gcagcgcgga ggaccggaga cgcgaagaua aacggcaagc cgacaaccag     1860

aagagaaaug aggaacgcaa acggcugauu cugucccuug gggagagacc gaccccucgc     1920

aaccuccuga agcugagacu cugggaggaa cagggacccg ucgagaaucg gcgcuguccu     1980

uauucuggcg aaacaaucag caugagaaug cugcuuuccg agcaagugga uauugaccac     2040

auccugcccu uuagcguuuc ucucgaugac uccgcugcca acaaaguggu cugccugcgg     2100

gaagcuaauc gcaucaagag aaaccggagc ccgugggagg ccuucgggca ugauucugag     2160

agaugggccg gcauucuggc ucgcgcggaa gcccuuccua agaauaaacg guggagauuu     2220

gcucccgacg cccucgagaa gcuggaagga gagggcgggc ugcgcgcccg gcaccucaac     2280

gauaccagac accugagccg gcuugcugug gaguaccugc gcuguguuug cccuaaagug     2340

agagucuccc ccggacggcu cacggcccug cugcgcagac gguggggcau cgacgcgauu     2400

cuugcugaag ccgacgggcc cccuccggag gugcccgccg aaacacugga uccuucuccc     2460

gcugagaaga accgcgccga ccauagacac caugcgcucg augcuguggu uaucggaugu     2520

auugaccgga gcauggucca gcgcgugcag cuggccgccg cuuccgccga aagagaggcu     2580

gccgcgcggg aggauaauau cagacgcgug cucgaaggcu ucaaagagga accuugggac     2640

ggguuucggg ccgagcugga aagacgcgcu cggaccaucg ucgugagcca cagacccgag     2700

cacggcauug gaggcgcccu ucauaaggag accgcuuaug ggccuguuga uccgcccgaa     2760

gagggauuca accugguggu ccgcaagccu aucgacggcc ucucuaaaga ugaaauuaau     2820

uccgugcggg accccagacu gcgccgggcc cugaucgaua gacuugccau ucgccggaga     2880

gacgcuaacg auccugcgac agcccuggcu aaggccgccg aggaccucgc ugcccagccc     2940

gcgagccgcg ggaucagacg gguucgcgug cugaaaaagg aaucuaaucc gauuagaguc     3000

gagcacggag gcaacccuuc cgggccccgg agcggcggac ccuuucauaa acuccugcuu     3060

gcuggcgagg ugcaccaugu ugacguggcc cugagagccg augggcggcg cugggucgga     3120

cacuggguga cccucuucga agcucacggc gggagaggag ccgacggcgc ggccgcuccu     3180

ccccggcugg gcgaugggga gcgcuuccug augagacuuc auaagggaga cugccugaag     3240

cucgaacaca aaggccgggu gcgcgucaug caaguuguga agcuggagcc uuccucuaau     3300

agcguggucg ugguugagcc gcaucaggug aaaacagaua gauccaagca cgucaaaauc     3360

ucuugugacc agcuccgggc ccgcggggcu agacggguga cgguugaucc ccugggacgc     3420

gugagagucc augccccugg cgcucgggug gggaucggag gcgacgccgg ccgcaccgcg     3480

auggaacccg ccgaggauau uagcuga                                         3507


<210>  4024
<211>  3513
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4024
augagcaagu acaacguggg ccuggacauc gggauugccu ccgucggaug gucuaucguu       60

gaugcugaca aaaagaaaau uaucgaucuc ggcagcagaa uuuucuccuc ugggaaugcc      120

gcggcuaacc aggagcggcg cggauuuaga ggcacccggc gccugaucag acggcgcaag      180

aauagacuua cagacgugau caacuucuuu ggggaaaaga aguucauuga uacgaaggug      240

gucgacaaaa auggacacgu gcauuauaag uuuagcguua acaauaccga gucccccuac      300

gaacugcggg ugaaaggccu cucugagauc cugacaaagc aagaacuugu cauugcccug      360

uaucacaucg ugaaacaucg cggcauuagc uacgaucucg gggaccugga ggaugacgga      420

accuccggcg uuacagauua uaagaccagc aucaacauua auagacagcu gcucaacgag      480

aagacagugg ggcagaucca gcuggaacgg cuuaaugagu ucggaaaagu ccgcggccaa      540

guggaagaga acgagaaugu gacccugcuc aacgucuuuc cuucugccgc uuacguuaag      600

gaagccacgc agauccugac caaacagaga gaguucuauc cggaaauuac agacgauuuu      660

aucgcuaagu ucaugcuuuu uauuacccgg aaacgcgacu acuucguggg gcccggaaau      720

gagaagucca gaacagacua cggcaucuau aaaaaggaug gccggacccu ggacaaccuc      780

uuugaugaac ugauugggau cgacaagauc aacggagaga aaagagccag cgcgucuucc      840

cugacggccc agauuuacaa uaugcucaac gaucugaaua accuuacagu gccuaauacc      900

gaggacggcc gccugacaau ccaaaccaag aaagaaauuc ucgaggcugc caagaccaca      960

aacgggcugu ucggaaucgc ucagauuugc aaaaucaucg gcuguaagaa ggaugacauu     1020

aaaggguucc ggaucgauaa gagcgagaaa aagauuaugc acacgcuuuc uggauauaga     1080

aaauuucgca aggaacuggc cgacgucaau aucgaagcga acgaucucaa uauuaaccug     1140

gugaauaaga ucgccgacau ucugacccuc aacacagaga aagcugagau ccggaagcag     1200

cuggacaaau ccaauaugga uaucagcaac gaaaugaagg agauuguuau caauaaaaac     1260

aaggaguucc uuguggacgg cagagccacc uggcauuccu uuucuuacaa gacacugaac     1320

cuccugauuc ccgaacuucu gaauaccccu gaggaacaga ugacgauccu cacacgccug     1380

ggccugguca aacccaacaa uaagaaauau gaggggcuca agaacauucc ugcucggaga     1440

aucaccgaag agaucuacaa uccggugguu gcccgcgcca cccgggaagc ugugaacguc     1500

uucaaugcga uuaccaaaaa ggugggaaga gauaacauca aagacgucau uaucgagcug     1560

ccccgcgagg auaaugaagu ggacauuaag aagggcaucg uuaaacggca aaagcagaac     1620

gagguggaaa aagagacagc cgaugcucuu gucaagcagc acaugacaau cagcgacucc     1680

gcccugauug cccaauauag aaaaauccgg gggcucagcc agaaggugcg cuacugguac     1740

cagcaggaua cgauuugccc uuauugugga aagaaaaucg aagcuguuca acugauuaau     1800

aacaaugacu cuuuugaggu ggaucacauc guccccaucu ccgugagcuu cgacgauucu     1860

cagaacaaua aggugcuggu ucauagccag ugcaaccagg ccaaagagaa gcaaaccccu     1920

cuuggcuggc ucaacaaugg gggcggauuu ggccagucca aagcggacua cauugcuaga     1980

gucaagucua acaagaauua ugccaaaaac aagaucgaca aucugcugaa cgaaacagau     2040

cucaaugaca uugccaccca gcggggguuc auccagagaa accugaauga uacacgcuac     2100

gcuagccgga uugugcuuaa cgaguuuuau uccuucuuua gaagcaauaa ccugucuacc     2160

aaagugaagg ucgugcgcgg aaaauggacg ucccaaauga gaaagaagug gaauggcguu     2220

gggggacuca gcaaaacccg ggacacauac caccaucacg ccaucgaugc uuguauuauc     2280

gccugcuucc cccugcugaa ggcguuugac aaagccauca aacuuauuga uguggacggc     2340

gaaaccgggg agauccucca ggauaaaaag gcuauuaaac uggcccagga agcugagauc     2400

gucgagaaga uuucugugau caagaacaaa aaguucgaaa auaugguuaa cgagaucuau     2460

aacuucccgc uguuuaaaca gguggaacuc gccaaugaca uuacaaaccu ggagaauccu     2520

gucaaguucu cccaccaugu ggauaaaaag gcgaaccgcg ccguugcuaa ucaaaccauc     2580

uacggcacga gaauuaagga aaaagaggug acaaagcggg gcaaaaagga gaaaauccag     2640

gaacgcuaca cccuuggaac aauuaagaac aucuaugaca ucgauggguu uaugaauuuc     2700

aagaaacugu acgacaagau uagcaaaaac gacgauguca aguuucucau gcagaaaaau     2760

gaccccaaga ccugggagaa gcugaucgau auuuucaacu ccuauccuga cucugaagag     2820

gugacccaag uggauggaaa agucaagaga guugccguga gccccuuuaa acuguacuau     2880

cgggagaaug gcuucaucac aaaguacucc aaacacaacg acgggcccaa gguggucucu     2940

auuaaguauu acgauagcaa agugggaucc cauaucgaca uuacgccuaa ggaugcucgc     3000

aauaaaguua uccuucagag ccucaagccg uggagaaccg acguguacuu uaacucuauc     3060

acacagcagu augaacugcu gggccucaaa uacuccgauc ugaaguucac caaugggguc     3120

uauggcauua cacgggacgc cuacgagaaa cuuaagcacg gagugagcga agacggcguu     3180

accgccugga agcccaucuc uaaaaacucc gaguuucgcu ucagccugua uagaaaugau     3240

cgggugagag ucauugacga aaagggggau ucuaucgagc uccuguuugc uagccgcacg     3300

acauccaacg agggauacgu ggaacugaaa ccuauuuaua aggcgaaauu cgacagcaag     3360

gaggaaguug gcuucuacgg gcuugugucu aacggacggc ucaucaagaa aauguccaag     3420

ggcggguaca aacuguauaa ggucaauacc gauauccugg gcaaacccua cuaugugacc     3480

agagagagcg acgaaccuaa gcucucccug uga                                  3513


<210>  4025
<211>  3516
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4025
auggccaagu acagcguggg ccuggacauc gggauuuccu cugucggaug ggcuaucauu       60

gauaucgaga ccaaacagau uguuaaccuc ggcagcagaa ucuuccccuc cgggaaugcc      120

gcgggaaacc aagaacggcg cggcuuuaga gggauccggc gccugauuag acggcgcaag      180

aauagacuua cagaccugaa aaagcuccug ggagagacgu ucuuuucuga ucuuaaaaag      240

aucgacagca acggcuauga auacuaugau auugacuucc agaucaaugc uaacccuuac      300

gagcugcggg ugaagggcau uaccgaaaaa cucuccaaga aagagcuggc cgugguccug      360

caccauauca uuaagcaccg cgggaucucu uaugaucucg gagaccugga ggaugacggc      420

acaagcgggg uguccgauua caaaaauagc aucaacauua auagacagcu ucugcgggaa      480

aagaccguug gacagaucca acucgagcgc cugauugaau uuaaccaggu gcauggccag      540

gucaagaaag ccgaggacgu gucucuucug aauguuuucc cguccagcgc uuauagagau      600

gaggccaaca agaucaugau uaaacagcaa gaauuuuacu cugagaucac aaauuccuuc      660

auggaacagg uggaccagcu cgucagccgg aagcgcgacu acuuuaucgg gcccggaaac      720

gagaaaucua gaaccgauua uggcauuuac aaguccgacg gccggacacu ggauaaucug      780

uucgcugaac ucaucgggac cgacaaaauu aacggagaga agagagccag cgcguccucu      840

cugacggccc agguguauaa ccuucugaau gaucucaaca aucugcacgu gagcaacacc      900

gaggacggca agcucacaac cuccacaaag aaggaaauca uuaaagcugc caagaccacg      960

gauggggucu uuggaaucag ccaaaucugc aaaauuaucg gcuguaagaa ggaggaaauu     1020

acaggguucc gccaggacaa aucugagaag aaaguuaucc auacccuggc uggauaccgg     1080

aagauuagaa aagcccucgc gacaaucaau auugaagcca acgagcugaa uccuaccgug     1140

uuugaucaga ucgcugacau ccugacccuc aacacagaga aggccgaaau ucgcaagcaa     1200

cuggauaaag ugcagcuuga caucacggac gagaugaagg aacuggucau ugauaaaaau     1260

aaggacuucc ucguggaugg ccgggcuacc uggcacuccu ucagcuauca gacacugaac     1320

augcuuaucc ccgagcugcu caauaccucu gaggaacaga ugacaauucu gaccagaaug     1380

ggccugauga aaagcgacaa cgagaaguac cgcgggcuca agaaaauccc ugccgaaaag     1440

aucgccuccg agauuuuuaa ucccguugug gcuaaaucua cgcgggaagc gaucaagguc     1500

uucaacgcca uuagcaaaga gaucggaaag aauaacauug agcacguggu uaucgaacug     1560

ccuagagaug acaacgagga agaugagaaa cgcaagaucc aaaaacuuca gaaagcuaau     1620

gaaacagaga aggaggccgc cgacaaccug gugaaacaua agcucggcau uuccgauagc     1680

gcucugaucg cccacuaucg gaaauuuaag gggcugucuc agaaggucag auacugguac     1740

cagcaagaaa ccuauugccc guacugugga aaaauuaucg aggcgaccca gcuuauuaau     1800

aacaaugaca acuucgaagu ggaucauauc auccccauuu ccgucagcua ugacgauucu     1860

cagaauaaca aggugcucgu ucacucccag ugcaaucaag cuaaagagaa gcagacaccu     1920

cugggcugga ucaacaaugg gggcggauuu ggccagagca aaaacgaaua caccaaucgc     1980

gugaagaaca auaagaaaua uuccaagaac aaagucgaca accugcucaa ugagacagac     2040

cugacggaua uuaucaccca gcggucuuuc auucaaagaa accuuaauga cacacgguac     2100

gccgcccgca ccgugcugga ugaguuuuau agcuucuuua aguccaacga ccuccccaca     2160

aaaguuaagg uggucagagg gaaguggacc ucucagaugc ggagaaaaug gaauaacgug     2220

ggaggccugc gcaagacgcg ggauacccac caucaccaug cuaucgacgc cagcauuauc     2280

gcuuccuucc cucugcuuag agccuuugau cgcgcgauca aacucauuga cguggauaag     2340

gaaacagggg agaucguuca guacggaaaa gccagccuga auggcgacgu cauugaagag     2400

aagucuguga uccaagcucc cauuaucaaa aacgaaaagu ucgagaagcu gguggaugag     2460

aucuacaauu uuccgcuuuu caaacagguc gaacuggcca acaauauuga gaacccugaa     2520

aaucccguga aguucuccca ccauguugac aaaaaggcua acagaaccgu ggccaaucag     2580

acaaucuaua gcacccggau uaaaaagacg gagaagaucg uccgcaaaaa ggugacagcg     2640

aaagaggaag aguacguuau ugggaccauc aaggauaucu augacgugac aaacuuugaa     2700

aaauucaaga aacuuuacga cgagucuucc aaaaaggaug acauuaaauu ucugaugcag     2760

aagaacgauc cuaaaaccug ggaaaagcuc gucgagauca ugaauaccua ucccgacuac     2820

gaggaaauua gccaacugga uggcaaggug aaaagaguua aggugucucc cuucaaacug     2880

uauuuucggg agaacggauu caucacaaag uacgccaaac acaauaacgg cccuaagguc     2940

gugagcauua aguacuauga cuccaaacuu gggaaucaca ucgauauuac gccggugaac     3000

gcuaauaaca aggucauccu ccagagccug aaacccuggc gcaccgacgu uuacuauaau     3060

aacgaaacac agcaguacga gcugcucgga cugaaguauu cugaucuuaa auuuaccaag     3120

ggcguguacg ggaucacaag agaagccuau gagaaacuga aguacggaua caccgacggc     3180

gugccugcuu ggaagcccau uuccaaagag agcgaguuca aguuuucccu cuaucggaau     3240

gaucgcguca gaaucauuaa cgaagccggg gaaaagaucg accugcuguu cggcucucgg     3300

aaugagacga gcgaaggaua cguggagaug aagccuauuu uuaaaacaaa cuucgacgau     3360

gccgaggagg cuuccuuuua uggcaagacc ucuaauaagc gccuuaucaa aaagauggcg     3420

agaaaagggu accaaaucua uaagguuaac accgacauuc ucggaaaucu guacuauaca     3480

gccaaagaaa gcaacgaacc caagcuggau cucuga                               3516


<210>  4026
<211>  3519
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4026
augagaaagc ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

aucacagaug acuauaaagu uccuucuaag aaaaugcgga uucagggcac gaccgaucgc      120

acaagcauca agaaaaaucu cauuggggcu cugcuuuucg acaacggaga gaccgccgaa      180

gcgacaagac ugaagcggac cacacgcaga cgguacaccc gcagaaagua ucggaucaaa      240

gagcuccaaa agaucuuuuc cucugaaaug aaugagcugg auauugcuuu cuuuccgcgc      300

cuuagcgaau ccuuccuggu gucugacgau aaagaguuug agaaccaccc caucuucggc      360

aaucucaagg acgaaauuac guaccauaac gauuauccua ccaucuacca ccugagacag      420

acacuggccg acagcgauca gaaagccgac cuccggcuga uuuaucuugc ucuggcccau      480

aucauuaagu uucgcgggca cuuccucauc gagggaaauc uggauuccga aaacaccgac      540

gugcaugucc uuuuucugaa ucucgugaac aucuacaaua accuguucga ggaggacauu      600

guugaaacag cuagcaucga ugccgagaag auucugaccu cuaaaacguc caagagcaga      660

cggcucgaaa aucugaucgc ggagauuccc aaccagaaac gcaauaugcu uuuuggcaac      720

cuggugucuc ucgcccuggg ccuuacaccu aacuucaaga ccaauuuuga acugcucgag      780

gacgcuaaac ugcaaaucuc caaggauagc uacgaggaag accuggauaa ccuccuggcc      840

cagaucgggg accaguaugc ugaucuuuuc auugccgcga aaaagcuguc ugacgccauc      900

cuccuguccg auauuaucac agucaaggga gcuagcacca aagcuccccu uuccgccucu      960

auggugcaga gauacgagga acaccaacag gaccuggccc uccugaagaa ucugguuaaa     1020

aagcagauuc cugagaaaua uaaggaaauc uucgauaaca aggagaaaaa uggcuacgcc     1080

ggguauaucg acggaaagac cagccaggag gaauuuuaca aauauauuaa gccgauccuc     1140

cugaaacuug acggcacaga gaagcugauu uccaagcucg aacgggagga uuuccugaga     1200

aaacaacgca cguuugacaa cgggagcauc ccccaccaga uucaucugaa ugaacuuaag     1260

gcuaucauuc ggagacagga gaaauucuac ccuuuucuca aggagaacca aaagaagauc     1320

gaaaagcugu ucaccuuuaa aauccccuac uaugugggac cucuggcgaa uggccagucu     1380

uccuucgccu ggcucaagcg ccagagcaac gagucuauua cacccuggaa uuuugaagag     1440

gucguggauc aggaggcuag cgcccgggcc uucaucgaaa gaaugaccaa cuuugacaca     1500

uaccugccgg aggaaaaagu gcuuccuaag cacucucccc uguaugagau guucaugguc     1560

uacaaugaac ucaccaaagu uaaguaucaa acggagggca ugaaacgccc uguguuucug     1620

uccagcgagg auaaggaaga gauugugaac cugcuuuuca agaaagaacg gaaggucaca     1680

gugaaacagc ucaaggagga auacuucucc aaaaugaagu gcuuucauac cguuaccauc     1740

cugggggugg aggacagauu caaugcuagc cugggaacau aucacgaucu ccugaagauu     1800

uuuaaagaca aggccuuccu ugaugacgag gcgaaccagg auauccugga agagaucguc     1860

uggacccuca cacuguuuga agaccaggcu augauugagc gccggcuggu gaaauacgcc     1920

gauguuuucg aaaagucugu gcuuaaaaag cucaagaaaa gacacuacac gggcuggggg     1980

cgccuguccc aaaagcugau caacggaauu aaagacaagc agaccggcaa aacaauccuc     2040

ggguuucuga aggacgaugg agucgccaau cggaacuuca ugcagcuuau uaaugacagc     2100

ucucuggauu uugcuaagau caucaaaaac gagcaggaga agaccauuaa aaaugaaucc     2160

cucgaggaaa caaucgccaa ccuggcuggc agccccgcca uuaagaaagg cauccugcaa     2220

uccauuaaga ucguggacga gaucgucaaa auuauggggc agaaucccga uaacaucgug     2280

auugagaugg cgagagaaaa ucagucuacc augcagggaa ucaagaacag ccggcaacgc     2340

cuuagaaagc ucgaggaagu ucauaaaaau acgggcucca agauucugaa agaguauaac     2400

gugucuaaua cccagcugca gagcgaccgg cucuaccugu aucuucugca agaugggaag     2460

gacauguaca caggaaaaga acucgauuau gacaaccugu cccaguacga uaucgaccac     2520

auuaucccuc agagcuucau caaggauaau ucuauugaca acaccguccu gacaacccag     2580

gccuccaaca gaggcaagag cgacaaugug ccgaacaucg agacgguuaa uaaaaugaag     2640

ucuuuuuggu auaaacaacu uaaguccggg gcuauuagcc agcgcaaauu cgaucaucug     2700

acaaaggccg agcggggcgc ucucucugac uuugauaagg ccggauucau caaaagacag     2760

cugguggaaa cccgccagau uacaaagcac gucgcgcaaa uccucgacag cagauucaac     2820

uccaaucuga ccgaggauag caaaucuaac cggaauguga agaucauuac ccuuaaaucc     2880

aagaugguga gcgacuuucg caaggauuuc ggcuuuuaca aacugagaga aguuaacgac     2940

uaccaucacg cccaggaugc uuaucucaau gccgucgugg ggacagcucu gcugaagaaa     3000

uaccccaaac uugaggccga auuuguguau ggagacuaca aacacuauga ucuggccaag     3060

cucaugaucc agccugacuc cucucugggc aaggcuacga cccggauguu uuucuacagc     3120

aaccucauga auuucuucaa aaaggagauu aaacuggcgg acgauacaau cuuuacccgc     3180

ccccagauug aggucaacac agaaaccggg gagaucgugu gggacaaggu uaaagauaug     3240

caaacgauca gaaaggugau guccuauccu caggucaaua uugugaugaa aacagaaguu     3300

cagaccggag gcuucucuaa ggagagcauc uggcccaagg gggacuccga uaaacuuauu     3360

gcccggaaga aaucuuggga cccgaagaaa uacggcggau uugauagccc uaucauugcu     3420

uacuccgugc uggucguggc caagaacugu aagcgcaaaa auagcaagac caaaaacuau     3480

caaagaacau gcggcaacaa gaauaacggg acccgguga                            3519


<210>  4027
<211>  3522
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4027
augagaccca ucgagccuug gauucugggc cucgacaucg ggaccgauag ccugggaugg       60

gccguguucu ccugcgaaga gaagggcccg cccacagcua aagaacuucu cgggggaggc      120

guccggcugu uugacucugg gcgcgaugcc aaggaccaca cgagcagaca ggcggagcgg      180

ggagcuuucc gcagagcccg gcgccaaacc agaacauggc cuuggcggcg cgauagacug      240

auugcccuuu uucaggcugc cggccugaca ccucccgcug ccgaaacccg gcagaucgcg      300

cucgcccugc gcagagaggc uguuucccgg ccccuggccc ccgacgcucu cugggccgcg      360

cugcuucauc uggcccacca ucgcggcuuc agaucuaacc ggauugauaa aagagagcgc      420

gcugccgcua aggcccucgc caaagcuaag ccggcgaagg ccaccgcuaa agccacagcc      480

cccgcuaagg aagccgacga ugaggcgggg uuuugggaag gagcugaggc cgcccugcgg      540

cagagaaugg cugccagcgg cgcuccuacc gugggggccc uucuggcgga cgaucucgac      600

cgcggacaac ccgugcggau gagauacaau caguccgauc gcgacggcgu cguggccccu      660

acgcgggcuc ugaucgccga ggaacuggcu gagaucguug ccagacaguc uagcgcguau      720

cccgggcucg acuggccggc cgugacccgc cugguccuug aucagcggcc ucugagaucc      780

aaaggagcug gccccugugc cuuccucccu ggcgaagacc gcgcucugcg ggcccuuccc      840

acagugcaag auuuuauuau cagacagacc cuggccaacc uccggcugcc cagcacaucu      900

gcugacgagc cucgcccgcu gaccgaugag gaacacgcga aggcccucgc ucugcuuucc      960

acggccagau ucguugagug gcccgcccug cggagagcuc ucgggcugaa acgcggagug     1020

aaguuuacag ccgaaaccga gcggaauggc gcgaagcagg cugccagagg gacagccgga     1080

aaccuuaccg aagcuauucu ggccccucuc auccccggcu ggagcgggug ggaccuggau     1140

cgcaaagaca gagucuucuc ugaucugugg gcggcucggc aggaccgcuc cgcccuccug     1200

gcccuuauug gagauccuag aggccccacc cgggugacag aggacgaaac ggcugaggcc     1260

guggcugacg ccauccaaau uguccugccg accggccgcg cgagccucuc ugccaaggcu     1320

gccagagcua ucgcccaggc cauggcuccu gggaucggau acgaugaggc gguuacacug     1380

gcccugggcc uucaucacuc ccaccggccc cgccaggaaa gacucgcucg gcugccuuau     1440

uacgccgccg cucugcccga cguggggcuc gauggagacc cugugggacc cccgccugcc     1500

gaggaugacg gggcggcugc cgaagccuau uacggccgca uuggaaauau cagcguccau     1560

auugcucuga acgagaccag aaaaaucgug aaugcccuuc ugcaccggca uggccccauu     1620

cucagacugg uuauggugga aacaacccgc gagcugaagg cgggggcuga ugagcggaaa     1680

agaaugaucg ccgaacaagc cgagcgcgaa cgggagaacg cugagaucga cgucgaacuu     1740

agaaaguccg aucgcuggau ggccaaugcu cgggagagac gccagcgggu gagacucgcc     1800

cggcgccaga acaaucugug ccccuauacg ucuacaccua uuggacacgc ggaccugcuc     1860

ggcgaugccu acgacaucga ucacguuauu ccgcuggcua gagggggacg ggacagccuu     1920

gacaacaugg ugcuguguca guccgaugcc aauaaaacca agggcgacaa gacccccugg     1980

gaggcuuuuc augauaaacc uggguggauc gcccaacgcg acgauuuccu cgccagacug     2040

gacccccaga cagcuaaggc gcuggccugg cgguuugcug augacgccgg cgaaagaguc     2100

gcccgcaaaa gcgcugaaga ugaggaccag ggauuccuuc cucggcagcu caccgauaca     2160

ggcuacauug ccagaguggc gcugcgcuau cugucucucg ucacgaacga gcccaaugcu     2220

gugguugcca ccaacgggcg gcugacagga cuucugagac ucgccuggga caucaccccu     2280

ggccccgcuc cgcgcgaucu gcugccuaca ccccgggacg cccuuagaga cgauaccgcg     2340

gcccgcagau uccucgacgg gcugacgccu cccccgcugg cuaaggccgu ggaaggagcu     2400

guccaagccc ggcucgcugc ccugggccgc uccagagugg cggaugccgg gcuugcugac     2460

gcccugggcc ucacccuggc uagccuggga ggcgggggca aaaaccgggc cgaucaccgc     2520

caucacuuua ucgacgccgc uaugauugcg guuacaacca gaggccuuau caaucagauu     2580

aaccaggccu cuggggcugg acggauccug gaucucagaa aguggccucg cacaaauuuc     2640

gagcccccuu accccaccuu ucgggccgaa gugaugaagc agugggacca uauucacccc     2700

agcaucagac cugcccaccg cgauggcggg ucccugcaug cugccacggu cuucggcgug     2760

cggaacagac cggacgcgcg ggugcucguu caacgcaaac ccgucgagaa gcuguuucuu     2820

gaugccaaug cuaaaccucu gcccgccgac aagauugcug aaaucaucga cggauucgcc     2880

ucuccuagaa uggcgaaacg guuuaaggcc cuccuggcuc gcuaucaggc cgcucacccc     2940

gaggugccuc cggcccuggc ugcccuugcg guggccagag aucccgcuuu cggcccucgg     3000

gggaugacag ccaacaccgu cauugcugga cgcagcgacg gcgaugggga ggacgccgga     3060

cugaucacac ccuuuagagc caauccuaag gcggcugugc ggaccauggg caacgccguu     3120

uacgaagugu gggagauuca ggucaaaggc cgccccagau ggacccaucg ggugcucaca     3180

agauucgauc gcacgcagcc ggcuccuccu cccccucccg aaaaugcccg gcugguuaug     3240

agacuccgca gaggggaccu gguguauugg ccucuugagu ccggagaucg gcuguuucuc     3300

gucaagaaaa uggccgugga cggccgccug gcucuguggc cggcccggcu ugcgaccggg     3360

aaggccacag cucuguacgc ccaacucagc ugccccaaca ucaaucugaa cggagaucag     3420

ggcuauugug uucagucugc ugaagggauu agaaaagaga agauccgcac cacauccugc     3480

accgcccucg gacggcugag acuuagcaag aaagcgacgu ga                        3522


<210>  4028
<211>  3525
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4028
augggcaagu acagcaucgg gcuggacauu ggaaucuccu cugugggcug gagcauuauc       60

gauauugaga aaaagaaaau cgucgaccuc ggguccagaa ucuuccccuc uggaaacgcc      120

gcugccaauc aggaucggcg cggcuauaga gggacccggc gccugauuag acggcgcaag      180

aacagacuug cggacguuau caauuuucug ggagaagaga acuucauuga uacaaaagug      240

guggaccaaa auggcaacau ccacuacaag cuccugguca auagcacgga auccccuuau      300

aaacuucggg ugaagggccu gucuaagaug cucaccaaac aggagcuggu uauugcucug      360

uaccauaucg ugaagcaccg cgggauuagc uaugaucucg gagaccugga agaugacggc      420

acauccgggg ucaccgauua caaaacaagc aucaacauca auagacagcu ucugauuaag      480

aagaccgugg gacagaucca acucgagcgg cugaaagagu uuggcaaggu ucgcgggcau      540

gugaaaaagg acgaaaacau uacacuucug aaugucuucc cgucuuccgc cuaugugaaa      600

gaggccaccc agauccucac gaagcagaga gaauuuuacc ccgagauuac cgaugacuuc      660

aucacaaaau uuaucccuuu cauuagccgg aagcgcgacu acuuuguggg acccggcaac      720

gagaagucua gaaccgauua uggcaucuac aaaaaggacg ggcggacacu ggauaaucug      780

uucgacgaac ucauuggaau cgauaaaauu uccggcgaga agagagcuag cgccucuucc      840

gucaccgcuc agaucuauaa caugcugaau gaccuuaaca accugacggu uacaaauacc      900

gaagauggga aacucacaac ccaaaccaag cgcgagauca uugaagccga caagacaacg      960

aacggacugu uuggcaucac ccagauuugc aaaaucauug gguguaagaa agaugacauc     1020

aagggauucc ggauugauaa aguggagaag ccuaucaugc acacacuuag cggcuacaga     1080

cgcuuucgga aggagcuggc gaaaaucaau auugaagcca acgaccucga caucaaucug     1140

gugaacaaga uugcugauau ccugacccuc aauacagaga aagccgaaau uagaaagcag     1200

cugaacaaau ccaauaucga cgucucuaac gagaucaagg aacuuauuau caauaagaac     1260

aaagauuucc ugguggacgg ccgcgcuacc uggcauagcu ucuccuauaa gacgcucaau     1320

cugcuuauuc ccgagcugau gaacacaagc gaggaacaga ucaccauucu cacccggcug     1380

gggcugguua aaucuaauuc cgagaaguac aaaggacuca agaacauccc ugccagacgc     1440

aucacagaag gcauuuauaa cccggugguc gcgaaagcca cccgggaggc ugugaauguu     1500

uuuaacgcca ucgcuaagaa gauugggaga gauaauauca gcgacguggu cauugaacug     1560

ccccgcgagg auaacgaggc cgacaucaaa aagggaaucg ugaaacggca aaagcagaau     1620

gaacuugaga aagaagccgc ugaugcgcug gucaagcagc aaaugacaau uucugacagc     1680

gcccucgugg cucacuacag aaagguucgg ggccuguccc agaaagugcg cuacugguau     1740

cagcagggga cgugcugucc uuacugcggc aagaacaucg aggccgucca acugauuaau     1800

aacaaugauu cuuucgaggu ggaccacauc auucccauca gcguuuccuu ugaugacagc     1860

cagaacaaua aagugcuugu ccauucucag uguaaccagg ccaagggaaa acaaaccccu     1920

cucggcuggc ugaauaacgg gggaggcuuc gggcagaaua agacagaaua uguggcuaag     1980

gugaaaacca acaagaacua cuccaaaaau aagauggaca accugcucaa ugauauggac     2040

cugagcgaua uuguuacaca gagaggauuu auccagcgga accuuaauga caccagauau     2100

gccucucgca ucguccugga ugaguucuac uccuuuuuca aaagcaacaa ucucuccacg     2160

aaggugaaga uugugcgggg caaauggacc ucucaaauga gaaagaaaug gaacgggguc     2220

ggcggacuga agaaaacacg cgacaccuau caccaucacg cgaucgaugc uagcauuauc     2280

gccuccuuuc cccugcuuaa gguguucgac aaagccauua agcucaucga uaucgacaga     2340

gaaacaggcg agauucugca ggauaaggaa gcuaucaaaa ugucucagga ggccaagcug     2400

cucgagaaaa uuccgguuau ccagaaugaa aaguuugagg aacugauuaa cgagcuuuac     2460

gacuucccuc uguuuaaaca aguggaacuc gcuaaugaua ucaccaaccc cgagaauccu     2520

gucaaguuca gccaccaugu ggacaagaaa gccaaccggu ccguugcgaa ucagacgauc     2580

uacgggacac gcauuaagga gaaagaaauc aacaagagag gaaaagugga gaagguccag     2640

gaacgguaua uucugggcac caucaagaac auuuacgaca ucgauagcuu cacaucuuuu     2700

aaaaagauuu augacaaaau guccaagacc ggggauguga aauuccugau gcaaaagaag     2760

gaccccaaaa ccuggggaaa acuuguugag auccucaaua gcuaccccga uucugaagag     2820

aucacacagg uggacggcaa ggucaagcgc gugaaagugu ccccuuuuga gcuguauaga     2880

cgggaaaacg auuucauuac gaaguacgcc aagcacaaug acgggccgaa aaucgucagc     2940

auuaaguauu acgauucuaa aaucggcagc cauauugaca ucacccccau caacgcuaag     3000

aauaaaguug ugcugcaguc ccucaagccu uggcgcacag auguguacuu uaacagcgag     3060

accagacagu augaacugcu uggacugaaa uacucugacc ucaaguucac aaauggcguc     3120

uaugggauua ccuccgaggc cuacgagaag cugaaacacg gaaucagcga agacggcacg     3180

aagacaugga aacccauugg gaagaacucc ggauuucggu ucucucugua ucgcaauaac     3240

agagugaaaa ucguugauga gaagggcgaa agccuugagc uccuguuugc uucccggacc     3300

accucuaaug aaggguacgu ggagcugaag ccuauuuaua aaagcaaguu cgacuccaaa     3360

gaggaugucg gcuuuuacgg acaaaugaca cccucuggcc aguucgugaa gaaaauggcc     3420

agaguugggu acaagcucua uaaggugaac accgacgucc ugggaaaucc guacuauauc     3480

acaaaagaaa gcgaugaccc ugagcuuucc cuguacaaga aauga                     3525


<210>  4029
<211>  3528
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4029
auggaggaaa ugaagcagac cggcagaaag aacgaggccc ccaagcugac agaccucaau       60

uaccggaucg ggcuggauau uggaaucgcu agcgugggcu gggccguccu ugaaaacaau      120

ucccacgacg agccuuuccg cauuaucgau cuggggguua gaauuuuuga cacggcggaa      180

caaccgaaaa ccggagcuuc ucucgcccug ccccggcgcg aggccagaag cgcucggcgc      240

agaauccggc gcagacggga ucgcaucgag agaauuaaga aacuuuucca ggccgaaggc      300

cugaucaaca uugacgaguu ugaagcucgg uauaagaucg ccgggcucuc cgauguguac      360

aagcugcgcu augagggacu ggacagaaaa cucacagaug acgagcuggc gcagguucuu      420

cugcauauug ccaagcaccg gggcuucaaa ucuaccagca aggcugaaga ggccgaaaau      480

gagaacgaug cugccaaaaa gaaggaagcg ggcgcccuca aaaaggggau cuccaauaac      540

aaaaagcuga uggaggagaa aggauacaga acagucggcg aaaugaugua ucuugacgag      600

aauuuucgca ccgauugccc uuggaacgaa aauggguaca uucugacacc ccggaacaag      660

ucuggagacu auaaacauac cgugcucaga gacaugcugg uugaugaggu gcgcgaaauc      720

uucaagagcc agcggucccu gggcaauauc cacgcuagcg aggagcucga aaacgccuac      780

cuggacauua ugcuuucuca aagauccuuu gauauggggc cuggagcucc cagcaaguac      840

gccauggagg gcuucgaaga ccgcgucggc cuguguacgu uugaggggaa aaauggagaa      900

aagcgggccg cuaaagcggc cuauaccucu gaacucuucg uggcucugga gaagaucaac      960

cauacaaaaa uuguuuccga ugacggcgcc accagauuuc uuacaaccua cgaacgcaag     1020

agcaucauug agcugaugca caagcagcag acggugaaau augccgcugu ccggaaggcc     1080

aucgggcucg cggaagagga uaaauucuac aaccugaauu auucuccuaa ggcuggaucc     1140

aaaaagagcc cggaggacac agauuuugug aagcuggaaa acuaccacaa aaucagaaag     1200

gcccucggcg aggacauugu guccgaacau cugcccgccg agaaaaucaa acuuuaugau     1260

ucuauugcua ccauccugac acucuucaaa aaugacgaca gccgcauuaa gcgguucauc     1320

gaacugggga ucggagagga gugcuuugau ggccuucugu cccucagccc uucuaaguuc     1380

cacaaccugu cccuggucgc caugggcaaa auuauccccu uucuccugga cgggaauacc     1440

uacgauaagg cuugugaacu ugccggauac gacuucaaag cggauaacaa gggcgagaaa     1500

agcguucugc ucaaggggaa aaauauuacc gacaugguga acgauaucac aaauccugug     1560

gucagacggu cugugagcca gacgauuaag guuaucaacg ccauuauccu ggaauaugga     1620

ucuccccaag cugugaauau cgagcuggcc cgcgaaaugu ccaagaacuu ugacgagaga     1680

cggaaacuua agagcgauau ggaguccaga gaaaaagaga auaacaaugu cauuaagcgc     1740

gugaaagaau acaagcucaa cccgaccggc caggacaucg uuaaguuccg gcugugggag     1800

gaacaggaua gacgcugccu guauagcggg gagaaaauug acaucaacga ucucuuuucu     1860

agagacggcg gauacgacgu ggaucauauu cugccuuauu ccaucacauu cgacgauacc     1920

uaccggaaua agguccuugu gaaagcuagc gagaaccgcc agaagggcaa uagaacaccc     1980

uaugaauacu uuauggccga gaaagacgau gacgaaugga acgucuacga gacccuggug     2040

cggaauacgg uuaaggauua uaagaaagug cucaagcugc ugaaaaagca caucacagag     2100

gacgaaaaau cugaguuccg cgaaagaaac cuuaccgaua ccaaguacau uacacggauc     2160

auuuauaaua ugauccgcca aaaccucguc cugaagccuu accugucccu cgagaaagaa     2220

agaaagaaac aggugauggc gguuaauggg agcauuaccc acuaucuguc caagcggugg     2280

ggacuugagu cuaaagaccg cagaacagau acgcaucacg cccaggacgc uguggucauc     2340

gccuguacca cagauggcau gaucaacaag auuagccggu acuccaaagg gagagagcug     2400

cucuauucuc gcggauuuaa guucguggac gaagagaccg gcgaaauccu gagccgggac     2460

aauuuuacaa gagaggaaug ggaugagaag uacgggguga aaauucccau ccccugggag     2520

cauuuccgcg acgaacugga uauucggaug uccagauaca acggcaccgg agacgauagc     2580

aucgacaaga cgaccccucg cgauuucauc cuuucucacc gggacgcuca uaaauggauu     2640

aauuauccgg aguggauguu uguugaaggc agacccgggc gccggggagu ccucgauggc     2700

aucuucgugu ccagaaugcc uaagcacaaa gugagcgggc aagcccacga ggaaacaguc     2760

cggucugcca aguacuauaa cgacggaggc uccguguuug agagcggggg cuacguugug     2820

accaagaaau cuauucagga uaucaagcug aaagacggag agauuaagga guucuuuaau     2880

cccgacagcg aucgccugcu cuauaacgcu cuggucgaca gacuuaacaa auucgagaau     2940

gauccuaaga aggcguuugc cgaacccuuc uacaaaccga aggcugacgg cacacagggg     3000

ccuaucguga aaaagauuaa aguuuuugag aagcagucca gcggaguguu cguccgggau     3060

ggcaagggga ucgccgccaa cggagcuaug gugcgcaucg acguguuuug cgaggaagau     3120

aaaggcaaga aaaaguauua cuucgugccc auuuacaccg ccgacgucgu gaaaaagaag     3180

cugccugaua gagcggcuac ggccaauaaa cucaucucug aguggcgggu gaugaaggaa     3240

gagaacuuua uuuucucccu guauagcaga gaccugaucu acauugaauc cgauaaaggg     3300

guccaaguua aggacaucga uggcaaaauc ucuaagcccg ugaaucuuuu cgccuauuac     3360

cucggagcug acacaagcac cgccuccauu ucuggcaaac ugcaugacag cuccgcgaac     3420

uuucgcucua ucgggauuca gggacugcgg agauucgaua agugucaggu ggacguccuc     3480

ggcaauauca gcuuugugaa caaggagaaa cgcaugccuu ucaauuga                  3528


<210>  4030
<211>  3531
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4030
augaacaagg ugcaggagga cugcuacaaa aagaucaaag aaaagcuggc cgauagagag       60

uaucggauug gccucgaccu gggggucgga agcaucggcu acgcuguugu gucccuuaaa      120

ggggauaugc ccgaagacgu gauucuggcc ggaucucgca ucuucaagag cuccauuggc      180

gcggcugaga gacggcacuu ucgcgcccaa agaaauaccc aucggcacca ucgcgaaaga      240

augcgguauc ucuggaagcu gcuugccgag aaaaaccugg cucuccagcu gccugagaau      300

cugucuaaga aagaaaacuu cacagauuuu gacacgagcc gcaagagauu cccgaucgag      360

guccuccgga augaucccua cauccugcgc uauaaagccc uugacgaaaa gcugucccuc      420

uuucagcugg gguacgcucu uuaucacauu gccaaccaua gaggaaccgc gucugugcgg      480

agcuuccugg aggaugacga ggccaagguu aaagaaaaua aggagacagc uaaacucgcc      540

aagaacacca aaaagaucau guacgagaaa aaguaccgca cauuuggcga auaucuguac      600

agagaacgga uugagaaguu cagacagaau gagcgcauga aagugaccaa caagaaugaa      660

aacaaagagu uugcugucac acgggauauc auugaaaagg agguggaaau gauccugggc      720

gcccagaaaa gauauaauac cgcgguucuc gccgacgauu acauugagcg caucaagaag      780

gcuaucuucu augagacgga aaaacugauu ccugagcccg aauacugucc uuauuuuaag      840

gacgacaaac ggcuucccag aucccacaag cugaacgagu accgccggau cuacgaagcc      900

cucaauaaca ccagauauua cauuccugag aucgaggaga aaacagggga aauucugagc      960

cgcaaggaaa aggagcaacu uucuaaagag gaaaaggaga aacuguucaa cuuucuccug     1020

aagaauaaag aacugaccga ggcuaaggug aagaaacucc ugaaccuuuc cgaaauggcc     1080

aagguccagc ugcagggacg ggauaaaaag aaucagaaaa ucaagggcua ugcccucauc     1140

acacuggaga gccuuccguu cuggucuaaa cugcccgagc aacagcagga cgaguuccuc     1200

uacgauugga acaagcugcc ugacgaaaag cugaaagaua gacuccgcaa uuauuacaag     1260

cugaccgacg augaaauuga cgagacguuu caaaacaucg ugcuucccuc caauuaugcu     1320

ccucugggga aaaaggcgau ggaaauucuc cuggaguaca ucaaaaacgg acugagcuac     1380

auugaggccc uugauaaggc ugccgacgaa aauaagauca aaguggagaa gcacgaaauc     1440

aacgaucucc ugcccuauua cggcaaaauu cugcaggagu cuacacagau gccgaucggg     1500

aaggccuucu ccaaacaauu ugcugccaag aauuauaaga aaccugacau uaacaagaau     1560

gaacaggagu ucggacggau cgcgaacccc gucguucauc agacccucaa ugagcugaga     1620

aaacuuauua acgaaaucau uacaguguuu ggcaagaaac cucuggagau cagccucgaa     1680

accucccggg agcugaagaa gucucagaaa gagcgcgaac ugaucgcuaa gcaacaggac     1740

auugaugagg agaaaagaaa gaaaaucugg aagaccuaca uugaacccca ccuucagcag     1800

aucaaaagcc gguccgacaa gcucaagaaa cuguucgugu uugaacugug ggaggaucaa     1860

aagcauaacu gccccuucug ucucgagaaa auuaaugucg acgauaucua uagcagaggc     1920

guggacaagg aacaccuguu uccucuuaaa aagucugagg augacuccga aaacaaucug     1980

guuaucgccc acaacaagug caaugcccgc aaagcugagc gggccccguu cgaugcuuuu     2040

gggcaugaaa gcauuuggaa cgacaucuuc ucuaaugccg agaagaaccu ccccagaaaa     2100

cuguggcgcu uuagacaggg cagcuucgag aaguuccugg aaaaaccucc caugcuuaag     2160

cgguuugaga cagauaauuc cuacauuucu aagaucagcg cgcgcuaucu cgccugucug     2220

uucgacaaac cuuccaagau cuuuugcgug aaacccuucc ugacggcuca gcucagaauu     2280

gccuggcagc uggaugaaau caugauucgg cuugcugaga acaucauucu ggacagcgaa     2340

aaggacucuu uuaauaaagg agggcuccaa aagaagaaac gcaucgauaa cagacaccau     2400

gcccuggacg cggucgugau ugccuacgcu ucccggggau accacaauuu ccugaaccgc     2460

guucaugcca agggcuauaa aaucaauuac gaugagaaga ccuggcuuag cgagacacuc     2520

agaccgccug ggaacaaaaa ccuggaagac uuuaagucug gaaucauuaa ugugaugauc     2580

aacgcuaaua uuuccaucaa gcacgaucac aacaauaaag gccagcuggu caagagcaaa     2640

gccuauaagc ucuacuauuc cgagauuggc aaauacauca uuaccacacu gaagucugug     2700

agcgaaaucc uuuucaaaca gaaggagaag ccccaggaca cccugcggcg cgcccucugu     2760

aaauuugagg auagacuguc cgacaucaag gauaaaaagc ugcggaaaaa gcuggaagac     2820

aacauuacga ucuauaaguc uauugagaau gaaagcgcuc uuuccaaagc gaagcaagag     2880

cucgaagagu ucgaucugaa agccaagaaa gaggggaaga agggauacga gaaaaucaca     2940

gacauucuca ucuacaagaa ggcuugcagc augcauggcg ggaaauauau ccacgucaaa     3000

aacagaauug aaaauaaguu uuucgcccug aaggaaccua ccgagaucga aaccggauuu     3060

ggcuacgaua cagccgacaa ccuuucucug gaccucuauc augauaauaa cgggaaacug     3120

uguggcgagg ugauucgcaa gaucgacuuc aaucggaaca gaguucccaa uuacaaaaag     3180

gagggauaua uucugcuuga acgcaucuac cagggcgaua cccuggaggu ggacaucucc     3240

gaugacaaaa uuagccucaa gaacaaaguc gggucugcuc cugauaagcg gacauucgug     3300

aagguucuga cguuuaccga auccgacaga uaucucaaaa acaagcgcga ucagauccaa     3360

auuuucuuuu gcaaucugcu uaaaagcaca cugaagcacg acgauucuag caaacacgac     3420

gacuccuucu acgugagcuc uaugcagaag uacaacgucc ggaaggugau ccucaccucc     3480

cugggaauua ucaaauauag aagcaagauu cugaaaaaua aggagaucug a              3531


<210>  4031
<211>  3531
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4031
augcuguucc ccaagaaacu cagcaaggag gaaguggccu acguccuggu ugagaucaac       60

uccaaucuua acaauucuag caacuaucug ggcaauauuu ccgacagauc uaaagaacuc      120

uacuuuaaca agcagaccau cgggcaauuc cuguauacaa aacuuaagga ggaucggcac      180

uacagccuga agaauaaagu guuuuaucgc caggacuacc ucgaugaauu ugagagaauu      240

ugggagacgc aggcuaucuu ucauaaggaa cugaccgagg accugaaaaa gcagauucgg      300

gauacaauca ucuucuauca acgcagacuc aaaucccaga agggacugau uucucuuugc      360

gaauuugaga acaaggagau caccauugaa aaagagggca agacagugac caaacagauc      420

gggguccggg ugauuccuaa gagcuccccg cuguuccagg aauuuaaaau cuggcaaauu      480

cucaauaacg uuaucuguaa gaaaaaggga acacgcaaga aaaaggccgu gacccccgcg      540

ccucugcuuu ucgagcuguu uggcacgccc agcgaaaccu ucuuuucucu cgacauggag      600

ugcaaacagc ugcuguucga ggaacucaau cugaagggga accuuaaagc ugauaagguc      660

aucagacugc ucggauacau uccucaggag ugggaaauca auuacaacca gcuggagggc      720

aaucggacac aaaagucccu uuaugaagcc uaccugaaaa uuaucgagau ugagggcuau      780

gacaucaaga ccuuccuccg caucaaaagc gauaaggaag acugggaucu ggaggaaucu      840

ccccuguccg ccaaagagau uagagaaaac auccagcaca uuuuugagac acucgggauc      900

agcaccgcua uucuguacuu cgacgccacg cuuccuauca aggauuuuga gaagcaggcu      960

ucuuaucaac uguggcaucu ccuguacucc uaugaaagcg acaacucccc gucuggaaau     1020

gagacacuuu acaaacugcu ccaggacaag uucggcuuua aacgggaaca cgccguguuc     1080

cugagcaaca ucuccuuuca ggaugacuac gggagccugu cugcgaaggc cauucgcaaa     1140

aucuaucccu acauuaagga gaaucaguau uccaccgcuu gugagcucgc cggauacaga     1200

cauagcaagc ugucucuuac aaaagaagaa cugaacaauc ggccucucaa ggaucugauc     1260

agcccccuga aaaagaacuc ccuucgcaau ccuguugugg aaaaaggccu caaccaaaug     1320

auuaaugucg ugaacgcucu gaucgagaaa aauucugaaa aggacgccga ggggaacauu     1380

aucaaguauu uccacuuuga ugagaucaga auugaacugg cgcgggagcu caagaaaaau     1440

gcccaggaaa gagcugagau gacccugaac aucaagaaug ccaccauuga acaggagaaa     1500

aucaagagca uucuuaucuc cgacuucgga aucaaaaacc ccacaaagaa cgauauuacg     1560

cgcuaccggc uguaucagga gcucgcucac aaugacuaca aggaucugua caccaacaca     1620

gacaucccga aagaacugcu uuuuagcaag aauauugaua ucgagcauau ugugccuaaa     1680

gccagagucu ucgacgacuc uuuuuccaac aagacccucg ccuaucgcga uuucaaucug     1740

aaaaagggcg aaaacacagc uuucgacuuu aucgauagcu cucugggcga ggaggagcuc     1800

aaucaauaca ucgcgcgggu ugagaacgcc uucaagacca aaaauauuuc cgcuacgaag     1860

uauagaaacc ugcuuaaaaa ggccacagaa aucaaugacg gguuuauuaa ccgcgaucug     1920

cgggagaccc aguacaucgc caaaaaggcu aaggaccucc uguuccagau uaccagaagc     1980

guggugucca caucuggaag caucaccgau cgccugcggg aagacugggg ccuuaucgau     2040

auuaugaaag agcucaauau gcccaaguau agagaccugg ggcugacaga gaucauugaa     2100

cgccgggaug gaagaaaagu cgaggugauc aaggacugga cgaaacggaa cgaucaccgc     2160

caucacgcca uggacgcgcu caccguugcu uuuacaaagc cuuccuacau ucaguaucug     2220

aaucaccuua acgccagaaa agacgaaaac aauaagcugu acgccgauau ccucggcauu     2280

gagcggaagg aaaccacaaa aauccaugac gagaacggga augagaagag aguguucauc     2340

gaacccauuc ccggauuucg ccaaaucgcu aaaaaguacc uggaggaaau ucugaucucu     2400

cacaaaacca agaacaaggu cgugacgaaa agcauuaaua agaucgccgg caccaacaaa     2460

aagcaggaga cacuuacccc ucggggccag cuccauaaag aaacaaucua ugggaaguac     2520

cacuauuacu ccaauaagga ggagaaaauu ggaaccaagu ucgauaaaga aacgaucagc     2580

aagguuucua acccgauuua uagauacgcu cugcagaaac gccuggaggc caaugaaaac     2640

aaucccaaga aggcguuuac aggcaaaaac gcccucucca agaauccuau cuaucuggac     2700

gaugcuaaaa ccaagauucu ucccgagaaa gugaaccuga ucuaccucga agaggacuuc     2760

agcaucagaa agaagauugu cucugagaau ccuacaucca aagaauuugu gcccaacagc     2820

aucucuauug agaagaucgu cgaugaaggg auuaaacgga uccugaagga gcgccuggag     2880

accuacaaca augacccgaa aaaggccuuc gcugaucuug acaaaaaccc uauuuggcug     2940

aauaaggaaa agggaaucag caucaaaaga gugaccauuu ccggcguuaa gaacacacaa     3000

gcccuccaua gcaaaaagga ucaccugggg aaacccaucc ucgacaagga gggccaggaa     3060

auuccugugg auuuugucuc uacgggaaau aaccaccaug uggcgaucua ucgggaccag     3120

aagggcaauc ugcaagagca gauuguuucc uucuucgaag ccgugguccg cgcucagcag     3180

gggcuuccca ucaucgacaa aaccuacaag caagaggagg gauggcaguu ucuguucaca     3240

augaaacaga acgaaauguu uguguucccu aaugagauua ccggcuuuaa ccccagcgaa     3300

aucgaucucc uggacccgaa gaauaaaaag cugauuuccc cuaaccuuuu cagagugcag     3360

aagaucucua uuaaagauua uuucuuccgg caccaucugg agacaaaugu ugaaaccaac     3420

aauauccuca aggacaucac guggaaacgc gggggacuga gccuccugaa gggcauuauc     3480

aaagucagaa uuaaccaccu ugggaauauc guguccauug gcgaguacug a              3531


<210>  4032
<211>  3534
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4032
augaacaagg ugcaggagau cgaauacaaa aagauuaaag agaagcuggc cgacagagaa       60

uaucggaucg gccucgaucu gggggucgga agcauuggcu acgcuguugu gucccuuaaa      120

aaguaugacg aucugucuua ccuccccgag gacaucauuc uggccgggag ccgcaucuuc      180

gcguccucua ucggagcugu ggaaagacgg gaguuucgcc uucaaagaaa uagccaccgg      240

caucgcagag agcggaugcg cuuccugugg aagcuccugg ccgaaaaaca gcuggcccuc      300

cagccuucca aggaucugga gaaaaaggaa aacucugcug acugcgagac cagccagaaa      360

agauuuccga uuaauauccu uaagaacgau cccuauauuc ugcgguacaa ggcccucgac      420

gagaaacugu cccuuuucca acugggcuau guccucuacc acguggcuaa ucaucgcggg      480

acagauagca ucagaucuuu ucuggaagac acggaugaca agauuaaaga gaacaccaag      540

acagccaaac uggcgaccga aacaaagaaa aucauugauu ccaagaagua ccggaccuuc      600

ggagaguauc ucuacaaaga acgcauccag cagaaugaga gaaagaaagu uagcaaccgg      660

aagacagccg gcaauaaaaa caaggaguuu gcugugacca gagacaugau cuucaaggaa      720

acggagaaaa uucuggccuc ucagaagcaa uauuacacca auguccuuac agacgaauau      780

aucaaagaga uugaaggcgc uaucuuuuac cagaccgaga agcugauucc ugaguccggg      840

uauugucccu acuucaaaga ugaaaagcgc cucccuugca gccacaagcu gaacgaguac      900

cggagaaucu augaagcccu uaauaacgcg cgcuacucug cccccgugcu ggacgagaaa      960

acaggagaaa uccucuccua ucaggagggc gaggaauuua gcuccgagga aaagaaaauu     1020

cuguucgaug agguucugcu caaggggaaa aagcugaccg agucugaaau caagaaacuu     1080

cugcaucucc cugacggaug ugagauucag cugcaaggcc gggauaagag aacgcagaaa     1140

aucaaggggu acgcucuuac agcccuggaa gcucucccgu uuuggagcaa acugucccag     1200

caacagcagg acgaguuccu guaugauugg aauggacucc ccgaagagaa gcugaaaccu     1260

aaacuuauug acugcuacag ccugaccgag aacgaagugg augagacauu ccagaagauc     1320

cgccuccccu cuaacuaugc cccugucggc aaaaaggcca uggaaauccu gcugaccuac     1380

auuaaagacg gcuacuccua uaccgaggcu cuugauaagg cggugagcga agggaaauuu     1440

aagguggaca agcaaacaac gaucuacgac ucucuccccu auuacggaaa aauucugcag     1500

gagagcaccc agaagcugau cggcaaagcc uucuccaagc aguuugcuaa acggaauuau     1560

aagaagccga acacaaauag aaacgagcgc gaguucggga aaauugccaa uccugucguu     1620

caccaaucuc ucaacgagcu gcggaaacuu aucaaugaaa uuaucaccgu guuuggaaag     1680

aagccccugg agaucggccu cgaaacagcc agagagcuga aaaagagcuc caaggaucgc     1740

gagagccugg ugaaacagca gaacgaaaau gaguacaacc ggaauaagau uuauaaagac     1800

uacgucgaac cucaccuuuc ucagaucaag uccagacaag agaaccccag ccgguacauu     1860

uucaaauuug agcuguucga agaacagaag ucuaucuguc ccuuuugccu gaaagaaauu     1920

accccugaug acaucaucaa uuccaaggug gauauugagc auaucuuccc gauugaagag     1980

agcgaggacg augcuaagaa caaucugguu aucgcccaca acgaauguaa cgcggacaaa     2040

gggaagaaau cccccuuuga ugcuuucgga gacaagacgu cuggcaaaua uguguggaau     2100

gauauucucc auaacgccaa gaagaaucug ccucacaaaa ucuggcgcuu uuaccagggc     2160

agccuugaga aguuccugga aaacaaaccc augcucaaga gauucgggac agacaauucc     2220

uauaucucua aaauugccgc uaaguaccug gccugccugu uuaacaauag cuccaagauc     2280

uucuguauua aaagcucucu uaccgcucag cuccggaucg ccugggagcu gaacuccauu     2340

augaucccuc uggcgaagaa uauccucagc gaaaaagagc uggaggaauu uaagacccuu     2400

aaaaacaaga agauuagaau ggauaaucgc caccaugccc uggacgcuau cacaauugcc     2460

uaugcuaacc ggggauacca caauuuccuc aacaaaaucc augccaaggg cuauaaaauu     2520

gacuacagag agaagaauug gcugucuaaa auccugcuuc ccccgcucaa ccgcgaucug     2580

gaagacuuua agauuuccgu caagaacagc aucgagaaag cgaauguguc uaucaagcac     2640

gaucauaaaa ccaacgggag ccugguuaag ggaacagccu acaaagugua uuacuccggc     2700

acggggaagu auauuaucac cacacucaaa aaugucgaag agauuagcuu caaggagaag     2760

gaagacccug aggauacccu gagacgggcu cuuugcggcu uugaagacuc uaacaucaaa     2820

gaugagaagc ugauuaaaaa gcucaaauac aauuccgacc uguauaagaa gauccugaac     2880

acaaaaccca aggccaaaca agagcuugaa caggcuaauc aggagaccaa ggaagaggga     2940

aagaagacgc aaaagaucac cgaugugcug auuuacaaaa aggccuguag ccucgucggc     3000

gaacaguaua uccagugcgc cucccgccug gagaacaaau ucuuugcugu gaaggagccu     3060

acagaaauua gaaccgggau cggauacgac acaggcgaua aucucuuccu ggaccuuuac     3120

uaugauaaaa cccggaagcu gugcggggag auuauccgca agaucgacuu uaaccagaau     3180

aaaauuccca acuaccaaaa ggaaggauau auccucuucg agagaauuua ccagggcgac     3240

acgcuggaag cggauacauc ugaggacaaa aucagccuga agaauaaaac cggguccgcc     3300

cuugauaacc ggacauuugu uaaggugcug accuucaccg agucugacaa auauuucgau     3360

ggcaagaagg acaaaauuca gaucuuuuuc ggaaaucucc ucaagagcaa cuacaaacag     3420

gaugacuccu uuuauauuuc uagcaugcaa aaguacaacg uccgcaaagu gauccucaca     3480

ucccugggca ucauuaagua cagaagccag auccuuaaga auaaagaagu uuga           3534


<210>  4033
<211>  3540
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4033
augagcaaga aaguguccag acgguacgag gaacaggccc aagagaucug ccagcgccug       60

ggcucuagac ccuauagcau ugggcucgac cugggagucg gcuccaucgg gguugcugug      120

gccgcguacg auccuauuaa gaaacagccg ucugaccuug uguucgucag cucccggauc      180

uuuauucccu cuaccggagc ugccgaacgc agacagaagc ggggccaacg caacagccug      240

agacaccggg ccaaucgccu caaauuccug uggaaacuuc uggcugagag aaaccucaug      300

cuguccuaua gcgaacagga ugugccugac cccgcccggc ugcgcuuuga ggaugcuguu      360

gugagagcca auccuuacga gcuccggcug aaggggcuua acgaacagcu gacacucucu      420

gagcugggau augcgcuuua ccauaucgcc aaucaccgcg gcuccagcuc ugucagaacg      480

uuccuggacg aagagaaauc cagcgaugac aagaaacucg aggaacagca agcuaugacc      540

gagcagcugg ccaaggaaaa aggcaucucu acauuuauug aggugcugac cgcuuucaac      600

acaaaugggc ucaucggaua ucggaacucc gaaagcguua aguccaaggg cgugcccguc      660

ccuaccagag auauuaucuc uaaugagauu gacgugcugc uucagacaca gaaacaauuu      720

uaccaggaga uccugagcga ugaauacugu gaccgcauug uguccgccau ccucuucgag      780

aacgaaaaga ucgucccgga ggcggggugc ugucccuauu uuccugauga aaagaagcug      840

ccccggugcc auuuccuuaa ugaggagaga cgccuguggg aagccauuaa caaugcucgg      900

aucaaaaugc cuaugcagga gggagccgcu aagagauacc aaagcgccuc uuuuuccgac      960

gaacagcgcc acauucucuu ccauaucgcc cggagcggca ccgacauuac gcccaaacug     1020

guucagaagg aguuuccggc ucugaagacc ucuaucaucg ugcuccaggg gaaagaaaag     1080

gcgauucaga aaaucgccgg auucagauuc cgccggcugg aggaaaagag cuuuuggaaa     1140

agacuuuccg aggagcaaaa ggaugacuuc uuuucugcuu ggacaaacac cccugaugac     1200

aagcgccuga gcaaauaucu caugaagcau cugcuucuga cagaaaauga gguggucgau     1260

gcccucaaaa ccgugucccu gauuggcgac uacggcccca ucgggaagac ggccacacag     1320

cugcucauga aacaccugga ggauggacuu accuauacag aagcucugga gcggggcaug     1380

gaaaccgggg aguuccagga acucagcguu ugggagcaac agucucugcu gccuuacuau     1440

ggacagauuc uuaccggcuc cacacaggcc cucaugggga aguacuggca cagcgcguuu     1500

aaggagaaaa gagacucuga aggauucuuu aagcccaaca cgaacuccga ugaggaaaaa     1560

uacggccgga ucgcuaaucc cgugguccac caaacccuga acgagcugcg caagcucaug     1620

aaugaacuga ucacaauucu uggcgccaaa ccucaggaga ucaccgugga gcuggccaga     1680

gaacucaagg uuggggcuga gaagcgggaa gacauuauca aacagcagac aaagcaagag     1740

aaagaagccg ugcuggcuua uagcaaguac ugugagccga acaaucugga uaaaagauau     1800

auugagcgcu uccggcuucu cgaagaccag gccuuugucu gccccuacug ucuggagcau     1860

aucuccgugg cggacauugc cgcuggcaga gccgaugucg accacaucuu cccucgcgau     1920

gacaccgcug auaacucuua uggaaauaag gugguugccc acagacagug caacgacauc     1980

aaagggaagc ggacgcccua cgcggccuuu agcaauacau ccgcuugggg accuauuaug     2040

cauuaucugg augaaacccc cggcaugugg cgcaagagac ggaaauucga gaccaacgag     2100

gaagaguacg ccaaguaccu ccaaucuaaa ggguuuguga gccgcuucga auccgacaau     2160

agcuauaucg cuaaggccgc caaagaguac cugagauguc uuuucaaccc gaauaacguc     2220

acagcugugg gcucucugaa gggaauggaa accuccauuc uccggaaggc guggaaucug     2280

cagggcaucg augaccugcu ugggagccgc cacuggucua aagaugccga cacauccccu     2340

acgaugagaa agaaccggga ugacaaccgc caucacggac ucgacgcuau uguugcccug     2400

uauugcagca gaucucuggu gcagaugauc aauaccauga gcgagcaggg caaacgggcc     2460

gucgagaucg aagcuaugau ucccaucccu ggguacgccu ccgagcccaa ccucagcuuu     2520

gaagcgcaaa gagagcuguu ccgcaagaaa auucuugaau uuauggaucu gcaugcuuuc     2580

gugucuauga agacagacaa ugaugccaac ggagcccucc ugaaggacac cguguauucc     2640

auccugggcg cugauacaca gggggaggac cuuguuuuug ucgugaaaaa gaaaauuaag     2700

gauaucggcg ugaaaaucgg agacuacgag gaagucgcca gcgcgauucg gggcagaauc     2760

accgauaagc agccuaagug guaucccaug gagaugaaag acaagauuga acagcuccaa     2820

uccaaaaaug aggcugcccu gcagaaguac aaagaaucuc uggugcaggc cgcugccguu     2880

cucgaggaga gcaaccgcaa gcugaucgaa uccgggaaaa agccgauuca gcuuucugag     2940

aagacgauca gcaaaaaggc ucuggaacuc gugggaggcu acuauuaccu gaucuccaau     3000

aacaaacgga ccaagacauu cgucgugaaa gagccuucua augagguuaa gggguuugcc     3060

uucgauaccg gaagcaaccu gugucuugac uuuuaucacg augcgcaagg caagcugugc     3120

ggggaaauua ucagaaaaau ucaggccaug aaucccuccu acaagcccgc uuauaugaaa     3180

cagggcuaca gccucuaugu gcgccuguac caaggagacg ucugcgagcu ccgggccucu     3240

gaccugacag aagcugaguc caaccuugcc aagaccacgc acgugagacu gccuaaugcc     3300

aaaccgggcc gcacauucgu uaucauuauc accuuuacag aaauggggag cggauaccag     3360

auuuauuucu cuaaccucgc uaaguccaag aaaggccagg auaccagcuu uacccugaca     3420

acgaucaaga auuacgacgu gcggaaaguc cagcugucuu ccgcggggcu ugugagauau     3480

gugagccccc ugcucgucga uaagaucgag aaagacgagg uugcccugug uggagaauga     3540


<210>  4034
<211>  3567
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4034
augaaguaca uccugggccu cgacauuggg gugagcuccg ucggaaucgc cguuauuucu       60

gaggaaaacc agaaaaagac caucgagaau cuggcuguga gaauugugcc cgaagauccu      120

gacuuccacg gcaaauuuua uagcgggaac acagccucca agaaucuuga gcggacggcg      180

aaacgcggaa acagaaagaa uaaccaacgg uucaaggcuc gcagagauaa acuguaccag      240

ucucucaagg aaaauaacau guuuccgacc agcaaucugu ucgaccuuuc cgccgucgag      300

cuguauggcc uccgggccaa agcugugucu gagcagguua gccugcagga acuggggcgc      360

auccucaucc ugcuuaacca aagacgggga uuucugucca aucgcaagag cgugucugag      420

gaaaacucca cagaguacaa agagagaauu gccggccucg aaaaggcucu gggcaauaag      480

accaucgggc agaaacuuua uagcgagcug caggaaaaca auaacgucuu cgaggugcuc      540

cugcgggaac gcacauacca gagaucuucc uauaccgagg aguuugaucg gauuugggac      600

gaacaaaaga aauacuaccu gauccucaca ggaagcucua acgaggauga caauaagggc      660

acccuguaug aucuuauucg caacagaauc auuuacuauc agcggccccu gaaaucccag      720

aaggggcuca ucagcgaaug ccuguucgag aaacaucaca aggccguugc gaaguccucu      780

ccuuacuuug aacuuuucag aaucuggcag aaaauuaaug accugagcug gaagacgccc      840

aacggagagc ucuuuaaacc uaccgaugag caaaagcaga aacugaagaa ugcccugugg      900

aacggcgaca aucucaaccc caagcauaaa cugacagugu ccaagaucaa agaaauucuu      960

ggguauacca gccgcgagaa gaucuaccug aauuucacag aacucgaugg aucucggacc     1020

uauuccauuc ugagaaacgc ucuuaucgag gccggcaucg aaaaaccuga gcaguaccug     1080

uuuuucaaua uggagacgaa cgacgaaaag ggcgggcucu uugagcugug gcacauuaca     1140

uacagccugc cgaucgaaaa ggaggucauu aaugcucuca aaaaccgcuu uggauucgac     1200

aagaaacaag ccgagaucau ugcgcggaga gugggcuauu cuagcgauua cgggucccug     1260

ucuacccgcg ccauccggaa acuucuggug caucucgaaa aaggacuggg cuauacagac     1320

gcuugugaua auaucgggua cgaccacagc ggauauaaga ccaagauuga ucugcagccc     1380

aaacuuaagc agcucaaaag aaacgagcug cgcaacccug ucguugaaca gauccugaau     1440

caagugguga acgccgucaa ucucauuauc gagaaguacg gcaaaauuga cgaaauccgg     1500

guggagcugg cuagagagcu ucgcaacaau gccaagaccc ggaaaaacau uucccugcuc     1560

aauagcaaga acaagaaaaa uaacgauauc aucagaaaga aacugcagga agaguauggc     1620

uuuaaguucg uuaaugggcg ggacgugaca cgcuacauuc ugugggaaga gaacucuaaa     1680

gaaugccuuu acuguaauaa gacgaucaac ggauccgaua ugcucaccgg caaugccgac     1740

auugagcaca uccugcccaa gagcagaucu uuuaacaacg cuaugaauaa cuauauuauc     1800

acacaucgga aaugcaauuc cgauaagggg cagaaaaccg cguacgacuu caugagcucc     1860

aagggagagg ucauccugca gcaauauauu gaaaaaguga acgaccucua caaggauggc     1920

aaggggucua ucagcaaaac aaaguuugag aaucugcuuu guaaaggcga agacauuccu     1980

uccgauuucg uugagagaau gaagaaagac ucucaguaua ucgccaagga agcugugaag     2040

augcugaaaa ccgucugccc caacguguac acgacaaccg gacaggucac cgauuuucuc     2100

cgcgaggagu ggaagcugaa aaaucugcuu caggaacuca cauucgagaa guauaaagcc     2160

auuggccaag ugaaggaaaa gaucaucaag accaacaaug ggcagacaaa gacguuuaaa     2220

guuauugccg acuggagcaa gcgggaugac cacagacauc acgcuaucga ugcccugauu     2280

ugugcgcuga ccgaccagaa aaucauuuuc aagcucaaca aucugaacaa aaucuaccag     2340

uacgagcgca augcucuuuc ccaaaaggag aucgaagaga uugaagaguu ucuggaagga     2400

aaguucagca ucaaagaguu cucugcccag cagggcaaca uuaucgagug cuccauuccg     2460

aauaucagaa aggaugccca aaaacaccuc gaaaacauuc ugaucagcuu uaagaaagag     2520

aacucuaagg ugcugacaau gaaucggaac cagccuaaga auuguaaaga acagaucacc     2580

uggguccccc gcgcuagacu ucaugaggac acaauuaugg ggcgggugaa gcgcaucccu     2640

gaaaagaaga ugaaacucaa cgauaagguu uccuucgagc agauuaagau gaucgugaau     2700

cccgagauua aagaaauccu gcuguauaga cucgccaagu uuggagacaa ccccaaaguc     2760

gcuuucgcca ccaaggcgcu gaaaaaggag ccuaucuuuu accgggaaaa ugagcuuaag     2820

gaagugagca uuuaugagug ggugaacacg aaacgcguug cccugaauga gaacaucacc     2880

gacucucaaa uugaaaagau cauugaucag gcuagcaaaa gagccgucaa ggagcggcuc     2940

aauggcggga aaaucaagga aguguucaaa aaccugacag agaauccgau ccagcugaag     3000

aacggcauga uucuuaagaa ugugaccguc acagacgagu ccaaagugga aaagauccgc     3060

aacggauuug cuauuaccgg caaaaaucac caugcccugg uuuacaagga ugagaacggg     3120

aaauauaacg acaagguggu cagcuucugg gaagcggugg ccaucggacu cagaaauguu     3180

acggaguacg gcaaccccua uccuauuauc aaucggaacc acaaugauga acugggggag     3240

uuuuacuucu cuaugcagau uaacgaccuc uuuguguucg aucugaagca uuccgagaau     3300

ccccaagaag agaacgaaau caauuucuuc gacgucgaga acagaaaaca gaucagcgau     3360

aaacuuuucc gcgugcagaa aauguccaag aaaucuaaug gacaguuugu uagcgacuuc     3420

cggcaccacc uggaaacauc cgugauuaga aacgauauga uccucaagaa uauuaccugg     3480

aucucuauua gcaagaacuc cgaccuggag cgccugacaa aaauccgggu caaucaucuu     3540

ggcgagauca uuaagguggg ggaauga                                         3567


<210>  4035
<211>  3585
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4035
auggacuuca ccgccgaugu gagcgagcug aacgaauccu uuaugcagau ccacacagag       60

ucuaaguucg aaauugacac ggcuaaaguc caagagauca gccagcuccu gcuugauaau      120

aagauuacca gaauggaccg gcagaaacag guugccaacg ugcuggcgaa gaauagcggc      180

gauaaagaag cugacaagau caacaagaaa auugccacag aguuuugcaa ggccgugcuc      240

gggaauaaag cuaaguucaa cgucauccug ggagaggaug ccguggacuc ugauuggagc      300

uuuaaaaugg acgaugaaaa uuccgacgag gaaaucgaga uucuuauggg ccaaaccaac      360

gggcagcagc agaauguucu gcgcaucauu caucaauggu acucucagau cacacucaac      420

ggaauugugc ccgauggcca gaugaucagc gaguccauga uugacaagua ugaacugcac      480

agaaagcauc ugcuccugcu uaaacagcaa aucaagguca ccaaagacaa gaaaaaggcu      540

cagcagcugc acgaggccua cgaacaauau gugcauggga caggagcgaa aaagaagcuc      600

acccaggaug acuucuacag caaaguuacg aagaaucugg agaccucuga uuuuuccgac      660

gaaaucaaaa agcagauuaa cgccgagaca uucaugccua aacagcggac cagcgcuaau      720

ggcgugaucc cgcaccaacu ucaccagcag gagcuggaua agauuaucga aaagcaggcc      780

aaauauuacc ccuuucucgc ugagcugaac ccugacacaa agcgccaaaa uauugccaaa      840

uauaagcugg augaacucau cgcguucaaa auccccuacu acgucggccc ucugauuacc      900

acggaggacc agaagaagac aucugggaaa uccuuugccu ggauggugag aaaggaaccc      960

ggaaaaauca ccccuuggaa cuucgaucag aagguggaca aaauggcuag cgccgaucag     1020

uuuauucggc gcaugacaac caaggacucu uaucuuauca ccgaggacgu ccugccggcu     1080

gagucccuca uuuaccaaaa guauaaaguu cugaacgaac uuaauaugau cagagugaac     1140

ggcaauaagc ugacaaaaca ugauaagcag aacaucuacc aacagguguu cgagaaauau     1200

aagacgguca augugaaaaa gguucaaaac uacuuccuca ccgacgggcg guaucagcac     1260

gcccccaaga uuacaggacu gagcgaugac aauaaauuua acuccaaucu gucuaccuac     1320

aucgauuuca agaaaauuuu ucaggacgug gucgaugacc cucaaaagca gaaagauuuc     1380

gaacgcauca uugaguggag cacaaucuuu gaagacagac auauuuacga ggugaagcuc     1440

aaggagaucu ccuggcugaa cgaugaacag cggaaagagc uuaagaaaaa gcgccaccag     1500

ggcuggggga gacugagcgc caaacuccug accggaaucu ucgacgaaaa uggcuauucu     1560

auuauggauc aacuuuggaa cacgcagaag aauuuuaugc agauccaggc ugaggagggc     1620

uucaacaaga aaauucaagc ggaaaauucc aagcaccugg agaaaaacga cuuugaagac     1680

auccucgagg augccuacac aagcccccag aacaagaaag cuauucggca gguuaucaag     1740

guggucgaag acauccagaa gaccaugggg accgccccuc agaaaauuuc uaucgaguuc     1800

acacgcagcg ccgauaagaa ucccagacgg uccauugaga gacagcgcca gcuggaagag     1860

aucuauuaca acgacgcuaa agaaauugug ucugagggaa ugcaagauaa ucugcagaac     1920

uuuaccgaca auaagcagac acucacggau aaauauuucc uguacuuuac ccagcuuggc     1980

aucgacaugu auacagggaa gaaaaucaac auugaugaaa ucggaaauua ccaaauugac     2040

cauauccugc cgcaggccuu cauuaaggau gacagcaucg auaacaaggu ccucgugucc     2100

agcgcgauca auaacggcaa aucugacaau guuccucugu ccauguacgc uagcgugcgg     2160

auuaagucug agguccagaa caaauggaag aauuucgccg agcacgggcu gaucucccag     2220

agaaaacuua acaaucucau gaccgacccc gaaagcauug auaaguauaa ggcccgcgga     2280

uuuaucaaac ggcaacuggu ggagacaucc cagguuauua gacugguggc ugaaauccuc     2340

aacgagaagu accagggcga aaccgagauu aucgaggucc gcgccaaaau gaacucucag     2400

cugcggaagg acuucgaucu ucugaaaaau agaaacguga augacuauca ucacgcucuc     2460

gaugccuacc ugacgaccuu uaucggcaac uaucuguacc gccgguaucc uaagcugaga     2520

agcuacuuca cauacgggca uuuuaagaaa auugacgaug cgaagcucaa acaauucaau     2580

uuucugcacg acaucgaaaa cgauaaggac cccaaauaug ugaaugauaa cggagagcug     2640

cucugggacc gcgaaaagga cauugccuac uucaagaaaa ucuauaauuu uaaguacaug     2700

cugguuuccc acgaggucuc uacccggcag ggcgcuaugu ucaaacagac aauuuuuccc     2760

gccgcugccg augccaagag caaacuuauc ccuaucaaga acgacaagcc gaccgcgauu     2820

uaugggggau acacgaauaa caaugaugcu uaucuggcca ucauuaaacu cgcuaagaca     2880

ggccaguuca aagugguggg ggucccccaa agagccaucg ugaagcugaa acaggccuac     2940

gacaaggaga ccggcgauua cuccaacguu cuguauaaag ugcuugaacc uaaguuuguc     3000

aagaaaaagg ugaaucggaa aacaggagag auuaccuacc agguugaaaa guucaaaauc     3060

guggucccca aggugccuua ucgccaacuc guuaucgacg gcgaucagaa guucaugcug     3120

gggagcucua ccuacaaaua uaacgcuaag cagcuggugc ucuccgagaa cgcgauuaaa     3180

auccuggaca agaaauccuu ugaucagucu ggauacaagu ccagcgaccu ugauacaguc     3240

uaugacgaaa uucugucuca aguggauaga uacuucagca uguacgacaa gaauaaauuu     3300

cgggagggcc uccauaacgg gaagaaauau uuccagagac ugcccaugau cgacaaugug     3360

gagaagggaa aaauuaagga aaacggcaag uuuaaacugc uuaaugagau ccucauuggg     3420

cugcacgcca acccguccuu cggcaaucuu aagagccucg gaaucucuac gccuuuuggc     3480

augaugcagu ucgcuuccgg gaucagccug uccgaaaacg ccauucuuug uuaccagucu     3540

cccaccggaa ucuuugagcg ccgggucgcc cugaaagauc ucuga                     3585


<210>  4036
<211>  3588
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4036
augcagaaga acaucaauac caaacaaaac cacauuuaca ucaagcaggc ccagaaaauu       60

aaggagaaac ugggcgacaa gcccuauaga aucgggcucg aucugggagu gggcagcauu      120

ggguucgcua ucgucuccau ggaagagaau gacggaaacg uucuucugcc uaaggaaauc      180

auuauggugg gcucucggau cuuuaaagcc agcgcggggg cugccgaucg caagcucucc      240

agaggacagc ggaauaacca ucgccacaca agagagcgga ugcgcuaccu guggaaagug      300

cuugccgaac aaaagcuggc ucucccgguc cccgccgacc uggauagaaa agaaaauucu      360

agcgagggcg aaacguccgc uaagcgguuc cugggcgacg ugcuccagaa ggauauuuau      420

gagcugcgcg uuaaaucucu ugacgaaaga cugagccucc aggagcuggg guacgugcuu      480

uaucauaucg ccggacaccg gggcuccagc gcgauucgca ccuuugagaa cgauucugaa      540

gaggcccaga aggaaaauac agagaacaaa aagaucgcug ggaauauuaa aagacugaug      600

gccaagaaaa acuaccggac cuauggagaa uaccucuaca aggaguucuu ugagaauaag      660

gaaaaacaua agcgcgagaa aaucuccaac gcugccaaua accacaaguu cagcccuaca      720

agagaccugg ucaucaaaga agcggaggcc auucugaaga agcaagcugg caaagauggg      780

uuucacaagg aacucaccga ggaguauauc gaaaaacuga caaaggccau uggauacgag      840

ucugaaaaac uuauccccga guccggcuuc ugcccuuauc ugaaggacga aaagcggcuc      900

cccgcuagcc auaaacugaa cgaggagaga cgccuuuggg aaacccugaa uaacgcccgg      960

uacucugauc cuauugugga caucguuacg ggcgagauca ccggguauua cgaaaagcag     1020

uuuacaaaag agcagaagca gaaacucuuc gacuaucugc ugaccggauc cgagcucaca     1080

ccggcccaaa ccaagaagcu gcuuggccug aaaaauacga acuuugaaga uauuauccuc     1140

caggggagag acaagaaagc ucagaagauu aaaggauaca agcugaucaa acuugagagc     1200

augcccuucu gggcgcgccu guccgaagcc caacaggauu cuuuucucua cgacuggaau     1260

agcuguccug augagaagcu gcugacagaa aagcucucca acgaguauca ccugaccgag     1320

gaagagauug acaaugcuuu caacgaaauc gugcuuuccu cuuccuacgc uccccugggc     1380

aaaagcgcca ugcucaucau ucuggagaag aucaaaaaug aucugucuua uacagaagcc     1440

gucgaggagg cccuuaagga agggaaacuc accaaggaga agcaggcgau uaaagaccgg     1500

cugccuuacu auggagcugu gcugcaggaa agcacccaaa agaucauugc caaaggcuuc     1560

agcccccagu uuaaggauaa aggcuacaag acaccgcaua cgaacaagua ugagcucgaa     1620

uacgggagaa ucgccaaucc ugugguccac cagacccuga acgagcuucg caaacugguu     1680

aaugagauua ucgacauccu cggaaagaaa cccugcgaaa uuggccugga gacagcucgg     1740

gaacugaaga aaucugccga ggauagaagc aaacuuuccc gcgagcagaa cgacaaugaa     1800

agcaaccgga acagaaucua cgagauuuau auccgcccuc aacaacaggu gauuaucacc     1860

cggagagaaa auccccggaa cuacauccuc aaguucgagc ugcuggagga gcagaaaucu     1920

cagugucccu uuugcggggg acagauuucc ccuaaugaca ucauuaacaa ucaggcugau     1980

aucgagcacc ucuucccgau ugccgaaagc gaggacaacg gccgcaauaa ccuggugauc     2040

ucucauuccg cguguaaugc cgauaaggcu aaaagaagcc ccugggccgc uuuugccucc     2100

gcggccaagg acucuaaaua ugauuacaac cggauccuua gcaaugucaa ggaaaacauu     2160

ccucacaagg cuuggagauu caaucagggg gccuuugaga aguucaucga aaacaagccc     2220

auggcugccc gcuuuaaaac agacaauucc uauauuucua agguggccca uaaauaccug     2280

gcuugccucu ucgagaagcc uaacaucauu uguguuaaag gcagccugac cgcgcaacug     2340

cggauggccu ggggacuuca gggccucaug auccccuuug cuaagcagcu gaucacggag     2400

aaggaauccg agagcuucaa caaagauguc aauucuaaca agaaaauuag acuggacaau     2460

cgccaccaug cccucgaugc caucgugauu gcuuaugccu ccagagggua cggaaaccug     2520

cuuaauaaga uggcgggcaa agacuacaag aucaacuaua gcgaacggaa uuggcugucu     2580

aagauucucc ugccgccuaa caauaucgug ugggagaaca uugaugcuga ccuggaaucc     2640

uuugagagcu cuguuaaaac agcccuuaag aaugccuuca ucagcgugaa acacgaucac     2700

uccgacaacg gggagcucgu caagggaacc auguacaaaa ucuucuauag cgaacgcggc     2760

uacacccuga caaccuauaa gaagcugucu gcucucaaac ugacagaccc ccaaaagaaa     2820

aagacgccua aagauuuucu ugagaccgcc cugcucaagu ucaaggggag agaauccgag     2880

augaaaaaug agaagauuaa aagcgcgauc gaaaacaaua agcggcuguu ugacgugauu     2940

caggauaacc uggagaaagc uaagaaacuu cuggaagagg aaaacgagaa guccaaggcc     3000

gagggcaaaa aggaaaagaa uaucaacgac gccucuauuu accagaaggc uaucagccuc     3060

uccggagaua aauaugucca gcugucuaag aaggagcccg gcaaauucuu ugccaucagc     3120

aagccuacac ccaccacaac cggguacgga uacgacacgg gcgauucccu cugcguggac     3180

cuguauuacg auaauaaagg gaaacuuugu ggagaaauua uccgcaaaau ugacgcucaa     3240

cagaagaacc cgcugaagua uaaagagcag ggcuucaccc ucuuugaaag aaucuacggg     3300

ggcgauauuc uggagguuga cuucgauauc cauucugaca agaauagcuu ucggaacaau     3360

acaggauccg ccccugagaa ccgcguguuc aucaaagucg gcaccuuuac agaaauuacc     3420

aauaacaaua uccagauuug guucgggaac aucauuaaga gcacgggagg ccaagacgau     3480

ucuuuuacaa ucaauuccau gcagcaguau aaccccagaa aacugauucu uagcucuugc     3540

ggguucauca aguaccgguc ccccauccug aagaauaaag agggauga                  3588


<210>  4037
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4037
augagcaagc acgugcuggg ccucgaccug ggggucggau ccaucggcug gugccuuauu       60

gcccuggaug agcaggacca acccgcugaa auccucggga ugggaucuag aauuguuccu      120

cugaccacag augaccuuaa cgaguucagc cagggcaaag ccaucuccaa gaaucagaaa      180

cggacgaccc agcgcacaau uagaaagggg cuggaucggu accaacuccg cagaaaagug      240

cugucugaaa agcuggcggg acauggcaug cucccggaca aggaucugau gcagcuuccc      300

gugcuggacc ucugggagcu gcgggcucgc gccgccagcc cuggcgaaaa acuuucccug      360

cccgagaucg ggagaguccu cuaucacauc aaccagaagc ggggauacaa acaugcuaag      420

ucugaugagg ccgaagaggu gaccaaacgc ggcaaggaac agggguaugu ugcuacagug      480

aagggaagau acgccgagcu gcaacgggag ggcgcgaccg ucgggcagca cuuugccugu      540

aaacugaagg aacaucgcca ggagagcccu uccggaggcu ucuuuuauaa uuacagaauu      600

aaagaccagg uguauccccg ggaggcuuac aucgaagaau uugaucgcau uauggagguu      660

caaagcgugu uuuacccuga gguccucaca ccggccuuca uccaggaacu gagagacaag      720

gugauuuucu uccagcggcc ccuuaaaucu ugcaagggcc ugguguccuu uugugaguuc      780

gaaaaacaag agagagucgu ucagcucaag cgcgugaacc gggaagggaa ggaggagcug      840

caggaaagaa aaaucaccau uggaccuaag guggcuccca aaagcucucc uaucgcccag      900

cuuugcaagc ugugggaguc cauccacaau auucgcaucu auuaccccga uggcaaagaa      960

cggccgauua cgcucgagga aaagagaaag cugcuggcgu aucuccaaac ccacgacaaa     1020

cugacauuua ccgcccuuaa gaaagagcug aagaucagcg acaaagagcg ccucuggugu     1080

gauaagcugc uuaagggagg aauuaaaggc gacacaaccc ggguccugcu cagaaaggcu     1140

cuggccgggu acaaacgcua ugaaccucug cuccggaugg aucuggagcu uagagacuuc     1200

caggaaaucg ugucuggaaa gcgccugauc cggcccauug uugaugaguc cuaccucagc     1260

cagccucugu auagacugug gcauauccuu uacuccauug aggaacggga ggcuaugcgc     1320

agagcccuca ucgaccagcu gggcuuucug gaagaggauc ucgacggcgg gcugcuugau     1380

aaacuguacc aaauugacuu cgccaagcag ggauauucua acaagagcgc uaaauucauc     1440

ugcaagcucc ugccccaucu gcagggcggg cacggauacu cccaggcgug ugaacuugcc     1500

ggcaaacggc acagcgcugg gucucucacg aaggaggagc uggaagagag accccugcuc     1560

gaacacaucc cucugcuuca augcaaugag cugcgccagc cgauugugga aaagauccuc     1620

aaccagauga uuaaucuggu caacgcccug cggcagaagu auggcuccau cgaugaggug     1680

agaguugagc uugcccgcga acucaaaaug agcagagagg aacgggagga aacaucuaag     1740

cgcauuagaa gccgggagaa ggagaauaaa gaaaucgcug agcgcauuaa ggaauacgag     1800

cuguauccca ccaaauccag aauccaaaag uaccggcugu ggcaggaggc cggagaacag     1860

ugucucuauu gcggccgcuc uaucgacgug guccaauuuc ugagagggga cggaguggag     1920

auugaacaua ucauuccuaa aagcauccuu uacgaugacu ccagcgcgaa caagacaugu     1980

gcuugcucuc ggugcaauaa ggauaaaggc aaccgcaccg ccuacgagua uauucuggcc     2040

gaagggagag aggcugagua ccucgaccgg cuggaaaccc uguuccacga uggaaaacuu     2100

uccuauagca aaagacgccg gcuccagugg aagaaagccg acauccccgu cgauuuuauc     2160

gagagacagc ugcgccugac acaguacauu gcucggcaag cccaggaaau ccucagacag     2220

ggcauucgcc gggugggggc gucugagggc uccguuacgg ccaagcugag acaucuuugg     2280

ggauaugacg auauccugcg cgaacucaau uucgagcggu accagagcau gggcgagacc     2340

gaaauugugu cccaagagga aaaggacggg ucuaucacaa aaagcgagag aaucaaggau     2400

ugguccaaac ggaaggacca ccgccaucac gcuauugaug cccuggucgu ggcuuguacc     2460

agacagggau auauccagcg gcugaacucu cuuaacagcg aagaggacuc ccugcagaug     2520

caagaggaca gcaaacagau gcgcgaaaag augggcgggc agaagagacc uacagccgau     2580

aaacacucua uucucgagaa uuggcugauc cuccaacccc auuuuuccca ccaggaaguu     2640

aagagcaaag uggcggagau ucuggucucu uuccggccgg gaaagagaac cguguccacg     2700

ggccgcaaca cauaccuucg gagcgggaaa aaggugaccc agucuggcgu ucugaucccu     2760

agaggacccc ucagcgagga aucccuguac ggccagaucc ugcgcaaugg ggagccuacc     2820

uaugucaaaa agguggcccu ugaaagccug ucugaggcuc aaauugaaca gaucguggac     2880

cccaagcucc ggagagagau ggucgcccgc acacagccuu ccgagggagc uaaacugaac     2940

cccaagcucc ugcuugccac cccguuuuac agcgaugccg cuggcacaca gcgggugaga     3000

uccguucgcg uggcgauuga aaaugggaaa aacucuaucg ucccucugag auauaaugag     3060

agcggaaagg ccauuacguu cgugaacccc ggcaauaacc aucacguugg gaucuaccgg     3120

accuccgaag gcaaacucgu ggagucuugc gucacauuuu ggcaagcugu ggaccgcguu     3180

agauauggga uucccaccau cauccggaag ccucgcgaag ugcuggauca gcugcuucug     3240

agagacgaua uuccggaggc ccuccuggcc caccuccccg cuagcgacug ggaguucguc     3300

gaauaccugc aggccaauga gauguuucuu aucggccuga cagaugaaga gauucagacc     3360

gcgcuggcug aauccgaccu ccugacgcuu ucuaaccauc uguaucgggu gcaaaagaug     3420

agcuccgagu acuacguguu cagauaucac cucgagaccg aagucgccga uaauaaaaac     3480

aauacaggga ucauuccuaa guuuuaccgc cugcggggac ccaacgacac cuaucucaaa     3540

gccaacccua gaaagguuaa agugaaucug cuuggcgaga ucagccugcc cuga           3594


<210>  4038
<211>  3615
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4038
auggagagaa uccugggcau ugacaccggg acaaacagcc ucggaugggc caucguggau       60

aaggacgaaa aucaguacca ccuggucgcu aaaggcacga acauuuucuc cgaggggguu      120

aagaucgaaa aaggaauuga gucuagcaag gccgcggaac ggaccgagca ucgcuccgug      180

agaaaacacu auuggcggcg caagaucaga aagauccggc uucugacaau ucucucugau      240

aaugaccugu gcccccgccu uaaacaugag caacugagac aguggcggcu caagaaagug      300

uacccuaccg augacacauu uauggaaugg cagcgcaccg aggauaaggc ucaggucaac      360

ccguauaaau ucagauacau cugucugaca caaaagcugg accucagcga uauuacccag      420

cgguuuaucc ugggccgcgc ccuuuaucac cugaaucaga gacggggguu ccucuccaac      480

cgcaaggaag ccacgaaaga gucugaggga accgugaagg aaggcauuag cucccugagc      540

aaagacauga aggauucugg cugcgaguac cuuggggaau auuuuuacca gcuguaccaa      600

cagggaaaga agaucagaaa ucauuauaca gcucggaaag agcacuaccu caaggaguuc      660

aaggccauuu guuccaaaca ggaacuggac gaugcucuga ucgagaagcu cgaaaaagcc      720

aucuuugacc agagaccccu gaaaagccaa aaaggccagg uugggaccug cacauucgag      780

aagggaaagu cucgcugucc uuccagccac ccccuuuaug aagacuuucg gauguacucu      840

uucauuaaca auaucaaagu gcaggucgau acccuguaug acaagggcga gagaucccuc      900

acggaugaag agaaacaacu gauuaucccu cuuuuuaagc gcaaaagcaa gcggacauuc      960

cccuuugagg acauugcgaa gaaaaucgcc gggaagaaca auuacugcua uaucaaaucc     1020

aacgaagaga aggcuuacaa auucaauuac cagauggaua cccagguguc uggaagcccu     1080

guuaacgccc agcuggaaga gauuuucggc gaagacuggc ucuccagcgu gugugagguc     1140

uauacacugg cuaauaacaa gucuagauuu caaaucauga acgaugugug gcaugugcug     1200

uucuuuuacg acgaugagga caagcucaaa gaguucgcca aggaucgccu gcagcuugac     1260

gacgaacugg cggaaaaauu uuccaagauu agcaucccga augauuaugc cgcucucucu     1320

cugaaagcca uucggaagau caugcccuac cuuagagacu auggccugau cuacagcgag     1380

gcugucuucc ucgccaaccu gguugaugug cugccucagc acgugugggg gauuaaagag     1440

augcgcgaag ccgcuaucga gaaugucauu gacgugaugc auuccuauga uaagaacaau     1500

aucgacggaa uuacccucga aggcugcguu aagaccuuuc ugaaagagcg guaccaggug     1560

ucugaugaca accuuaagaa acuguaccac cccagcaugc ucgauccuua ucccagacaa     1620

cgcccgaaug acgaaggggu cuuccagcug ggauccccuc ggaucagcuc ugugaagaac     1680

cccauggcga ugcacucccu guuuagacuu cggaaaguug ugaaucucau gcugaagaag     1740

ggcuacauua caccugauac gaaaguccau aucgaguuca gccgcgaccu gaacgaugcc     1800

aauaagagau gggcuaucca gaccuggcag cgggagaacg aaaaagagag agaacgcugu     1860

cggaaggaga uugaaaaaua uaucgccucu cccuccgaca cagacauucu caaguaccaa     1920

cugugggagg agcagaauca caagugccuu uauaccgggg augaaaucag aauuacagac     1980

uuucugggag auaaccccaa auacgacauc gagcauacca ucccucgcag cgccggcggc     2040

gauuccacga agaugaaucu cacacugugu ucuagcagau ucaaccggga cgaaaagaag     2100

accaaacugc cggcugagcu ugugaaccac gaugaaauuc ucgagcgcau cuccaauugg     2160

aaggagaagu augaagacau ugauaaacag aucagaaaga uuucuaccaa aggggccaca     2220

agcaaggagg acaaagauuc caagauccgg cgcagacauu accugauccu gcagcgggac     2280

uacuggcgcg gaaaauauca aagauuuacc augacagaag uccccgaggg cuucagccgg     2340

cgccagggga cggauauuuc ugugaucucc agauacgcgc ggauguaucu caagagcguu     2400

uucaaacagg uguacauugu caaaggaauc gcuaccucug acuuuagaaa gauguggggc     2460

auucaagacg aguaugccaa aaaggaacgc gugaaccacg uucaccauug caucgaugcc     2520

aucacaauug cuugcaucgg gaaagccgag uacgacaagc uggcugauua ucggcaccag     2580

uacgacgauu accuuuucca gggcaagacc aaaccuacau uugacaagcc cuggccuucc     2640

uucgugauug auaucaaaca uauucaggac gaacugcuca ucgcccacua ugcgaaggau     2700

aaucuggaga aauuuaccaa gaagaaccug agaggaaaag acggccgcau uauccgggau     2760

aaguucggga auaaacuuca aaugaaggga gacacgagca gaaacucucu ccacaaugac     2820

aaauuuuacg gcgccaucaa ccaugaaggg gagauuaagu augucuaccg caccaauauc     2880

gaggcucugg augccaagag cauuggaaac aucguggacc ccacagugcg guccauuauc     2940

gaaagcgcua ucgccauuca cggcucucug aaagcggcca ucaauaaggg gauuuggaug     3000

aacgaggaaa aacauauccc gauuaagaaa guuagagucu uccgcaauga ugugaagaag     3060

ccuaucgagc ucaaagacca gcgggaucug uccgacaagg auuauaaaca gcacuacuau     3120

gugaccaacg acggcaauua caugaugcuu aucuuuagca agcugaacaa caaaggaaga     3180

gaaauucggc agcaugucgu gaucaauucc cucgaggcua cagagguuau uaagucucac     3240

aaacgcagcc aagugggcaa ggucgugugg gaauccuacu auucuaagga gggguucgau     3300

cugaucuaca aacuuaagag cggaaccaug auucugcucu augaaaacaa uagagacgag     3360

guuugggauc uggacauccg gaaccugcag aaacgccucu acauuaucac gaagaugaaa     3420

uaugauggca gaaucuacau gaugcaccau caggaagccc gggagaagaa ggaccuggag     3480

gaaaaauccg ggucuuuuaa gauuaaugag gacuauagac cccuuaucgu gaacagcuac     3540

aaucaguuca acgcucuggu ccaagaucuc gacuuugaaa uuaaugaucu gggagagauc     3600

cgccggcuga uuuga                                                      3615


<210>  4039
<211>  3630
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4039
augaagaaag acuacauccu gggccucgau auugggauca gcuccguggg auggggccug       60

cuugagcugg acgauaacaa uaaccccuau aagauuaucg acgucggguc uagaauuuuc      120

accccuggag aaguugagaa aacaggcgau agccgggcca aggaacgcag agagaaacgc      180

ggggcucgga gaaucgcccg gcgcagagaa uuucggcucg accgcgugaa gaaucugcuu      240

uacgagaacg gauaucugaa gggcaauauc aacuccaaua uugugucuga gaaaaacgaa      300

gaacucagca ucauuuucaa uaacaugauc aauaacuacu auaauggcaa gaacacgaau      360

ccguacauuc ugaaagucga agcgcuggau agaaagcuca ccgacgagga gcuguccauc      420

auucuugugc acuaugcuaa aaagcggggg uacaagucua accgcgaaga ggccagcgau      480

aaagaauccg gaaagguucu gacagccauc aaagagaacg aaaagaucau gaaagacaau      540

aacuacagaa ccauuagcga gauguauauc aaggaugaca aauuuaagaa uaagauuaaa      600

aacucuccca augauuacaa gaucuccgug acaaacgaga uguaucucga agagauuaau      660

aaaguccugg acagccagau caaguucggc cuuaucaccg acgauuuuaa aaaccaauac      720

cuggaaauuu auaauucucg gcgcuccuac gcuaaggggc cuggaggcaa cagccccuau      780

gggggagacc ucaucgagag aaugacaggc aagugcaauu ucgauggcaa cccucgggcc      840

cccaaaagag cuuuuucuuc cgaaauuuac guggcccuga ccaagcuggu uaaucucaaa      900

uacaagguga acgacgggga guauaucaaa cugagcgaga aggaaauuaa gaauaucauc      960

gagaaagcga aggagaaaaa guccauuucu uacaaagauc uuacgaagau gaucggaaac     1020

aacauugacu ucaaggaucu gacccucagc aaaaaggagu auaucaaacu gauugaagaa     1080

cuuaagaaaa agcugaauau caaaaaggac acaaagauua acaucaauga gcucuccagc     1140

gaagauaaag agcuguacaa caagcugugu aaugacaaac uccugucuaa gacccuuauc     1200

gaacugaaag gcuaucauau ucucaaggau uccgucauca aggccuacag caaagacauu     1260

ugggacgagg ugaaggauaa cauguaucug cuugacgauc uggcucucua cugcacaaau     1320

uacaaaguga acgaggacau ccugaauaag auuaaagaau cugagaacau cgauaauauc     1380

uuuaacgaca uucacuucgu cgaacugcuc ccuaauuuua aggaucaucu gaugcuuagc     1440

accgagcuga uccgcaagcu cauuccgauc auguaugggg gaaacacgua cgacaaagcc     1500

augaauauuc ugggcuauaa ccacuccgau aucaacgggg acaaggagaa aaaggaucug     1560

cuuguuccca ucugugugga cgauaauauu acaaaccagc gggugaucag aucucucacc     1620

cagacacgca aagucauuaa uagcaucauu aagaaguacg gaaugccuaa aaucaucaac     1680

auugagaccg cucgggagcu ggccaagucc agacaggaac gcaaugagau cgcgaaaaac     1740

aauguggaaa agcaagccga gaacgagaaa auuaagaauc agcugguuga acucggccug     1800

uucgugagca ccgacaaaau cucuuccaca gaccuucuga aguauaagcu cuggaaagag     1860

cagaacgaau uuugcggcua cagcaugaag aaaauuucua ucgaggaucu guucgggaau     1920

aacauugucc agaucgacca uauccugccc uauucccgga cguacaauga uaacuaccuu     1980

aauaagaccc ucguguauac aaaagaaaac caagagaagg gaaacagaac cccuuacgaa     2040

ugguucggca agacagacaa augggaugag uaugagaccu acauuaauaa ccugacgauc     2100

agccagaaga aaaaggacaa uuaucugcuc aaaaagcugg auuuugacac agaacgcgag     2160

augcgguccc agaaccuuaa ugauaccaag uacauuucua aagaacugag cucccucauc     2220

aaggcuuauc ugaacguuga gaaagugaau auguaccccg gggccauuac cgcuaagcug     2280

agagcccgcu ggggauucaa ccggcuuaca cacucuuacc ucagcaaaaa uuauauccug     2340

ccgaagaaca ucaaggagga cauuaauaaa gauagagaca accaucugca ccacgccaug     2400

gaugcucucg ucaucgcguc cauuaccccu agccugcagc aaaagaucac acuuuacgaa     2460

aaauuuucuc gguauauuga cggccugacg gagcuccaga ucaagaauau cuccuacauu     2520

aaaaucgaag acagcgaggg gcaguauuac aacgaugaca ccgaugaagu ggagacaauu     2580

gacauccgcg aguauauuaa gcaacagcug gccgaaaaua acaucuacua caauagacau     2640

aacauuucua agcuggaguu cccccuuccu uaugaaaacu uugauaaaga ggcuaagcuc     2700

cgggucuacg aacaggaccu ggauacccug aaauggaauc ucaaguccua uaacacauac     2760

acccagaaag acaucgauaa ugugcacacg cugaucccca gcauugccaa gaccaaaguu     2820

ucuggcaaga ugcacgagga gacauauuac ggaaucaagg aaauuggcaa agacgaggau     2880

aagaaaaucu auaagacccu uagaacaccc auugaaaaag ugaagcgcaa ggaccuggac     2940

aacaucccug aucggcucgg gggaagcaaa gacgucuacg agucccuggu ggaaugguuc     3000

ggcgauuacg agguugggga ggaagugcug aaggccaaug gaaacaaaua uccggucagc     3060

aagucugaca aagagcauaa ggaaaucaag aaacuuaagc uguacuccga guauaaaaau     3120

accggccaca ugauuaacaa gagcaaugug gagaaagggg gcaucuacag aauugaugug     3180

uuuaagucca aggacaaaga agaugacaag cucuauuucg cugccuacga uauccuggag     3240

auuaaaaaga ucaacacgcu caaagaaaag aaucgcguug acgauaacuu uaauaucaaa     3300

cuggacuaug gacaaggcaa gaacucuaag auuaugagcu acacagauau cauuaauaac     3360

uacgaccuuu auaucucccu gaacaaaaau gaccucauua agaucaccac aaacgauggg     3420

aaagagucug ucgcguacgu ggugggaugu agcuccggca ugcuggaagu caagucuaaa     3480

cugggggacg gauaugauau uaucaagacc gaccuuaaug agaacaauau cuucagcaag     3540

cagagagcuc aguaccagau uaccgugucc acaaucaaag agauuaagaa acugagcauc     3600

ucuguucucg gcgaaauuuc cgggcuguga                                      3630


<210>  4040
<211>  3633
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4040
augagaacca uccugggccu cgaccugggg acaaacagca uuggaugggc cgugaucaau       60

uccgucauua aggagcagac ggaacggauc uggauugaga uggcuggcuc ucgcaucauc      120

cccauggaug ccgcgauucu uggggacuuc gauaaaggaa acagcaucuc ccaaaccgcu      180

gaaagaacac gguuucgcgg cguuagacgg cugcgcgaga gacagcuccu gcggcgcgaa      240

agacuucaca aggugcugaa aauucucggg uuccugccug agcauuaccu gaagggaauc      300

gacuuugaga aacacaccgg caaguuccuc ucuggcagcg aaccgaagcu gcccugggug      360

aaagaucggu gggggaagua uuccuuucuu uuccagacag ccuuuaauga gaugcuggcc      420

gacuucgcua aacaucagcc uucucucguc uuugauggaa agaaaauucc cuacgacugg      480

accguguauu accugcgcaa gaaggcccuu acagaaauga ucagcaaaga ggagcuggcu      540

uggauucucc ugaacuucaa ucaaaagaga ggcuauuacc agcugcgcgg ggaagaggaa      600

gaggauaacg ccggaaaauc cguugaauuu uaugcgcuca aggugcugag cgucgaggcc      660

accgacgaga aaaagggccg ggauaucugg uacaaugugc accuugaaaa cggguggguu      720

uacagacggu cuuccaaugu gccucuggac ugggagggaa gagucaaaga guucaucgug      780

acgaccgauc ucaaccccga cggcacaccu aagcuggaca aguauggcga ggugaaacgc      840

agcuuucggg cuccgaagga agaugacugg augcuuauua aaaagagaac cgaggccgau      900

aucacaggga cccauaaaac gauuggaucu uacaucuaug acgcucugcu ccaggagccc      960

cagcaaaaga uuaucggcaa gcuggucaaa acaguugaac gcaaguucua caaagaggaa     1020

cugaauuuca uccuccagaa gcagugcgcc uuucacgcgg agcugcagga ucgggaccuu     1080

uauaugagau guauugaagc ccuguacccu gggaacgagg ugcaacgccg gaauaucgcu     1140

aacagagauu ucauuuaucu ccugaucgac gaugugcugu uuuaccagcg cccccugaaa     1200

accaagaagu cccucauugc caauugcccu uacgaggaaa acgaguaugu caaccggaaa     1260

acaggagaaa ucaagaccgc uccccugaaa uguaucgcca agagccaccc gcuguuccag     1320

gaguuuagac ucugguacuu ccugucuaau auucgcgugu aucaaaaaga gcgggaaguu     1380

aacggcaccc uucaucugga cguggauguc acaacggagu uucucaagac cgaagacgac     1440

uacguggccc uguucgauug gcuuaauucc agaaaggaga ucgaccagaa agcguuucuu     1500

cgguauccug cuuucgggcu caagaaagaa auuaacaauu accgcuggaa cuauguugag     1560

gauaaguuuu accccugcaa ugagacaaga cacgccaucc ugagccggcu ggaaaaagcu     1620

ggaauuggca ucgacuuccu cuccgaugag aaggaaaccg cccuguggca uauucuuuac     1680

ucugugagcg acaaacagga gaucgaaaag gcccuggcua cauuugccgu caagaacggc     1740

cucaaugagu ccuucgugga ggucuucaaa aaguuuccuc ccuucaaaag cgaauauggg     1800

gcguacucug cuaaggccau caaaaagcug cugccccuua ugagaugugg aaaguauugg     1860

gauauguccc ucauugacgg caccacgaaa gagcgcaucg aacggauuau cgccggggag     1920

ugcgaugaaa gcauuggagc uaaggugaga gagaaagcca ugaaccuguc ugacaucagc     1980

uguuuuaagc gccugccucu cuggcuggcu ugcuacguug uguauaauag acacuccgag     2040

gauaaagaaa ucacaaagug ggagaccccg gccgacauug augcguaccu ugccgcuuuc     2100

aagcagcacu cucugcggaa ccccaucguc gaacaaguga uuaccgagac acuccgcguu     2160

gugagagaca ucuggaaaca ggucggcaag auugacgaga uccggaucga acuggggcgc     2220

gagaugaaaa auccugccga uaagagaaaa cggaugacca cacagauucu ggaaaacgag     2280

aaugcuaacc uucgcaucaa ggcccuccug gcggaauuug ugaaccccga guauggcgug     2340

gagaauguua gaccuuacag ccccucccag caagaaauuc ugcggaucua cgaggacgcc     2400

guccucaagg gagaagaaca gauuccggaa gauaucgacg ugauccugaa aaaguucaac     2460

acgagcaaac uuccuaccaa gucugaguuu cugcgcuaua aacucuggcu ggagcagaag     2520

uacagauccc ccuauacagg cgaacugauu ccucuugcua aacucuucac cgccgcuuac     2580

gagaucgaac augugauucc ccagagccgg uauuuugaug acucuuucuc caauaagguc     2640

aucugugaga gcgccgugaa caagcugaaa gauaaucaac ugggguacga auuuauuaag     2700

aaccaccaug gacagaaagu ugaggugggc uucgggaaga cagucgagau cuuuuccgug     2760

gacucuuaug aaagauucgu uaaagagcag uacgccaaga gcggaauuaa gaugaaaaag     2820

cuccugaugg augacauccc ugaacaguuc aucgagcgcc aacuuaauga uucccgguac     2880

auuucuaaag uggucaaggg ccugcucagc aacaucguga gagagaaaaa ugacuccggg     2940

gaauaugagc ccgaagcugu uagcaagaac auucuggugu gcaccggcuc ugucacggau     3000

cgccugaaga aagacugggg aaugaaugac guguggaacu ccaucgugag cccgcgguuu     3060

gagagacuua augcgcugac cgaaacacag uguuucggcc acugggagaa caaggauggg     3120

aaaaaggucu uucagaccgc cguuccucuc gaguaccaaa aaggauucuc uaagaagcgc     3180

auugaccauc ggcaccacgc uauggaugcc aucgugaucg ccugcgcuac aagaaaucau     3240

gugaacuauc ugaacaauga auccgccagc cgcaacgcga aaauuucucg guacgaccuc     3300

cagagacugc uuugcgauaa gagccggggc gacgggaaug gaaacuaucg cuggaucauu     3360

aaaaagcccu ggaccacguu uacacaggau gcuagagagg cccuguccgg caucauuguc     3420

agccucaaac agaaucugcg gaucaucaac aagaccacaa auauuuacca acacuucgac     3480

gccaacggga cccgcgugua ugaaaagcag aaaaucggcg auucuugggc uauuagaaag     3540

cccaugcaua aagacaccgu uuuuggagcc gugaaucugc ggaaggcgcu uaucccucag     3600

cacuacaacc ugacacucuu cauuuccagc uga                                  3633


<210>  4041
<211>  3642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4041
auggagaaga gacugggcau cgacgugggg accaacagcc ucggaugggc cauugucgaa       60

guugaugacg agggcugcac acugcuugau agaggggugg acaucuucca ggaaggagug      120

gcucgcgaga aaaaucaaga aaagcccgcc guccaggaua gaacggacgc gcgggcucug      180

cgcagacacu acuuucggcg cagacuccgg aaaauugccc ugcuucgcgu gcugguuaga      240

caugaucucu guccugcccu guccgaggag cagcugaacg cuuggcaggg caaggggauc      300

uauccgcucg acgaugccuu cauucggugg caacgcaccg acgauaaugc ugacagaaac      360

cccuaccggg aucgcuuuag augccugaca gaagaacuug accuggccug ucgggaagag      420

agauaugugc ucggacgcgc gcuguaccac cuuugccagc ggagaggcuu ccugucuaau      480

cgcaaagacg ccggcaacga ggcugaagag gggaagguca agcaggaaau caaaacccuc      540

agcgccgaua uggcugccgc gggaugugag uaucugggcg aauacuuuua ucggcuguac      600

cagagaaagg agaaaauccg caagcauuac acagcccgga augagcacua ucucgcugag      660

uucagagcca uuugccgcaa acaacggcug gacgaggaac uuagacaggc ucuggagaag      720

gccaucuuuu accagcgccc ucucaagucc cagaaagggc uggugggaaa guguaccuuc      780

gaaaaagaua agucucggug ccccguuagc cauccuagau uugaggaguu ccggaugcuu      840

caauuuauua acaaugugcg cgucacaggc cccggggacg ccgaaccuag accgcugucc      900

cccgaggaag uggagaucgu gcggccucuc uuccugagaa aaucuaagcc ccacuuugau      960

uucgaagaca uugcuaaaaa gaucgcggga aagggcaacu augccugucg cggcgagcug     1020

cuccaggcuc cuuaccgguu caauugcgcc gggaaaagca ccgucacggg aucccccguu     1080

agcgcccugu uugcuucugu guucggcgau gacuggcaga gagagauuug uucccuuuau     1140

acccuggggg ccggaaagaa cgaagaacaa auccucaaug auguguggca cguccuguuu     1200

gcguucgaag acgaggagcg ccuucaggcu uggcugcagg aaaaacucca gcugagcgcc     1260

gaugacgcca agacacuggc ugccuuuaga cucccgcaag gcuacgcuuc ucugucccuu     1320

aacgugauca auaaaauucu gccuuaucuc cggcgcgggu acagauacga cgaggccguu     1380

uucuuugcga accugggaaa ggugcugccc gccgaagucu augcugaucc ugcccggcgc     1440

agagaggugg aacagggcau cagcgcucag auggacgauc uuggcgacgg ggagaagacc     1500

cggaaagagg aacgcauuag agauuaccuc cggcgcuauu ggggaguuga ggaaaagaga     1560

cuggagcggc uguaccaucc cucuaugauc gaagccuauc ccuccgcgag cccuaacggc     1620

caggggcucc ugcaacuugg auccccgcgg auucccgccg ugcgcaaucc uauggcuaug     1680

cgggcccugu ucagacuccg cguccugcug aacgagcuuc uccgggaggg caaaaucgac     1740

agagggacaa agaucaauau ugaacuggcu cgcgagcuga acgaugccaa uaaacggaag     1800

gccaucgaaa gauaccagcg cgagcgggaa gcugagaaca gaaaguaugc ggaggaaauu     1860

cggcagcagu acguggaggc caccggccac ucuaucgaac ccagcgagga ggacauucuc     1920

aaauaccgcc ugugggucga acaaggacau ugcucccuuu auacgggcaa gacaaucggg     1980

aucagcgagu uucugggaga uggcagacgg uacgacauug aacacaccgu gccucgcucu     2040

agagggggag aggaugcuca guauaauaaa acacucugcg aagaggaguu caaccgggac     2100

aucaagagag auaaacugcc cuccgaacug gccaaucaca ccgccauucu ugcuuguguu     2160

gagagcugcg gcugguuuga acgcaucgag gggcucgaac aucgggugug gggcgccgac     2220

agacgcgcga agucugcuac cacaaagagc gccaaagacu ucgccauuca ggaucggcac     2280

uacuggagaa ugcaucugga cuauuggcgc cggaagugug aggcuuuuac gaugaccgag     2340

gucccggaag gauucuccaa cagacagggc guggauguug ggaucauugg acgcuacgcc     2400

agacuguauc uccaaacagu guucgaccgg gucuacaccg ugaaaggcgc gacaaccgcu     2460

gccuuucgca gaaugugggg gcugcaggag gaauacgccg cuaaggagcg ggugaaucac     2520

guucaucacu gcaucgaugc caucacgauu gcuuguaucg gaucucgcga auaugcccuu     2580

ugggcgcagu acgucagaca agacgagcug uaucgguggg gcgaggccga uaaaccuaga     2640

ugcgggaagc ccuggccuac auucaccgaa gacgugcgcg cuaccgccga ugagcuccug     2700

auuagccacc auacacccga caacaugccu aaacaguccc ggaagagacu gcgcgugcgg     2760

ggcaaggucc agagaaauga ucggggagaa gugaaauaca ugcagggcga caccgcucgc     2820

ggggcccuuc accaacagac auuuuaugga gccaucgaga gagauggcga gauucgguac     2880

guugugcgca agagccucga ccagcugcug cccgcugacg ucgagaaaau cguggaugag     2940

gcgguuagac agaaggugcg ggaagccguc gaggcuuaug gguucaaaga agccacggac     3000

ccgaccaagc auacagugug gaugaacgag gagaagcaaa ucccuauucg caaaguuaga     3060

auccucaccg gagugaagaa ucccauucug cuuaaacccc accgggaugu cucugugaag     3120

gaauacaaac gcuacuauca cguggucaac gacggcaacu acuguauggc caucuaugag     3180

gggagagauc ggcagggcag acugaagcgc acauuugaaa uuguuaauaa gcucgaggcu     3240

gcccgguacu ucaaaagauc cgcggaccgc gaaagccggc cugaucuggu gccgcugucu     3300

gacgugaacg gauauucccu uaaguaccug cucaaaaccg gcacgauggu ccuguuuuau     3360

gagaauagcc ccgaugagcu cuacgaaugc uccguggggg agcuggcuaa gagacuuuac     3420

aaaguugugg gaauggccca ggacggccgc guccaguucc uguuucauca agaagcccgg     3480

gaucagaagg cugugaccgc cgaguguggg augggaaucu cuguuuucga cgcgagacac     3540

ccugcuccca agcuccgcau ccgggugagc aacuuuaaaa uguucgucga aggcuaugac     3600

uuugagcuga cagugaccgg cgagguuaag uucaaaagau ga                        3642


<210>  4042
<211>  3642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4042
auggagaaga gacugggcau cgacgugggg accaacagcc ucggaugggc cauugucgaa       60

guugaugacg agggcugcac acugcuugau agaggggugg acaucuucca ggaaggagug      120

gcucgcgaga aaaaucaaga aaagcccgcc guccaggaua gaacggacgc gcgggcucug      180

cgcagacacu acuuucggcg cagacuccgg aaaauugccc ugcuucgcgu gcugguuaga      240

caugaucucu guccugcccu guccgaggag cagcugaacg cuuggcaggg caaggggauc      300

uauccgcucg acgaugccuu cauucggugg caacgcaccg acgauaaugc ugacagaaac      360

cccuaccggg aucgcuuuag augccugaca gaagaacuug accuggccug ucgggaagag      420

agauaugugc ucggacgcgc gcuguaccac cuuugccagc ggagaggcuu ccugucuaau      480

cgcaaagacg ccggcaacga ggcugaagag gggaagguca agcaggaaau caaaacccuc      540

agcgccgaua uggcugccgc gggaugugag uaucugggcg aauacuuuua ucggcuguac      600

cagagaaagg agaaaauccg caagcauuac acagcccgga augagcacua ucucgcugag      660

uucagagcca uuugccgcaa acaacggcug gacgaggaac uuagacaggc ucuggagaag      720

gccaucuuuu accagcgccc ucucaagucc cagaaagggc uggugggaaa guguaccuuc      780

gaaaaagaua agucucggug ccccguuagc cauccuagau uugaggaguu ccggaugcuu      840

caauuuauua acaaugugcg cgucacaggc cccggggacg ccgaaccuag accgcugucc      900

cccgaggaag uggagaucgu gcggccucuc uuccugagaa aaucuaagcu gcacuuugau      960

uucgaagaca uugcuaaaaa gaucgcggga aagggcaacu augccugucg cggcgagcuc     1020

cugcaggcuc ccuaccgguu caauugcgcc gggaaaagca ccgucacggg auccccuguu     1080

agcgcccuuu uugcuucugu guucggcgau gacuggcaga gagagauuug uucccuguau     1140

acccucgggg ccggaaagaa cgaagaacaa auccugaaug auguguggca cguccuuuuu     1200

gcguucgaag acgaggagcg ccugcagaca uggcuccagg aaaaacugca gcugagcgcu     1260

gaugacgcca agacccucgc cgcuuuuaga cugccccaag gcuacgccuc ucuuucccug     1320

aacgugauca auaaaauucu cccguaucug cggcgcgggu acagauacga cgaggcuguu     1380

uucuuugcca accugggaaa ggugcuuccu gcggaagucu augccgaucc cgcucggcgc     1440

agagaggugg aacagggcau cagcgcccag auggacgauc ucggcgacgg ggagcagaca     1500

cggaaggagg aacgcauuag agauuaccug cggcgcuauu ggggaguuga ggagaaaaga     1560

cuggagcggc ucuaccaucc uucuaugauc gaggcuuauc ccuccgccag ccccaacggc     1620

caagggcugc uucagcuggg auccccucgg auuccggcgg ugcgcaaucc cauggccaug     1680

cgggcucucu ucagacugcg cguccugcuu aacgaacucc ugcgggaggg caagaucgac     1740

agagggacca aaaucaauau ugaacuggcc cgcgagcuca acgaugcuaa uaagcggaaa     1800

gccaucgaaa gauaccagcg cgagcgggaa gccgagaaca gaaaguaugc ugaggaaauu     1860

cggcagcaau acguggaggc gacgggccac ucuaucgaac cuagcgagga ggacauucug     1920

aaguaccgcc uuugggucga acagggacau ugcucccugu auacaggcaa aaccaucggg     1980

aucagcgagu uucucggaga uggcagacgg uacgacauug aacacacagu gccccgcucu     2040

agagggggag aggaugccca guauaauaag acccugugcg aagaggaguu caaccgggac     2100

aucaaaagag auaagcugcc uuccgaacuu gcuaaucaca ccgccauucu cgccuguguu     2160

gagagcugcg gcugguuuga acgcaucgag gggcuggaac aucgggugug gggcgcugac     2220

agacgcgcca aaucugcgac aaccaagagc gcuaaggacu ucgccauuca ggaucggcac     2280

uacuggagaa ugcaucugga cuauuggcgc cggaaaugug aggccuuuac gaugacagag     2340

guccccgaag gauucuccaa cagacaaggc guggauguug ggaucauugg acgcuacgcu     2400

agacucuauc ugcagaccgu guucgaccgg gucuacacag ugaagggcgc caccacggcg     2460

gcuuuucgca gaaugugggg gcuucaggag gaauacgccg ccaaagagcg ggugaaucac     2520

guucaucacu gcaucgaugc uaucacaauu gccuguaucg gaucucgcga auaugcucug     2580

ugggcccaau acgucagaca ggacgagcuc uaucggagag gcgaggcgga uaagccgcgc     2640

ugcgggaaac cuuggcccac cuucaccgaa gacgugcggg ccacagcuga ugagcugcug     2700

auuagccacc auaccccuga caacaugccc aagcagucca gaaaacgccu ucgggugaga     2760

ggcaaggucc agcggaauga ucgcggagaa gugaaguaca ugcaaggcga cacagccaga     2820

ggggcucucc accagcagac guuuuaugga gccaucgagc gggauggcga gauucgcuac     2880

guugugagaa aaagccugga ccagcugcuc ccugccgacg ucgaaaagau cguggaugag     2940

gcuguucggc aaaaagugcg cgaagcgguc gaggccuaug gguucaagga agcuaccgac     3000

cccacaaaac auaccgugug gaugaacgag gagaagcaga ucccgauuag aaagguucgg     3060

auccugacag gagugaaaaa uccuauucuu cugaagcccc accgcgaugu cucugugaaa     3120

gaauacaaga gauacuauca cguggucaac gacggcaacu acuguauggc caucuaugag     3180

gggcgggaua gacagggccg ccucaaacgg accuuugaaa uuguuaauaa gcuggaggcc     3240

gcuagauacu ucaagcgcuc cgccgaccgg gaaagcagac ccgaucuggu gccucuuucu     3300

gacgugaacg gauauucccu gaaauaccuc cugaagacgg gcaccauggu ccucuuuuau     3360

gagaauagcc cggaugagcu guacgaaugc uccguggggg agcuugcgaa acgccuguac     3420

aagguugugg gaauggcuca ggacggccgg guccaauucc ucuuucauca ggaagccaga     3480

gaucagaaag ccgugacagc ugaguguggg augggaaucu cuguuuucga cgcccgccac     3540

cccgcgccua agcugcggau cagagugagc aacuuuaaga uguucgucga aggcuaugac     3600

uuugagcuga ccgugacagg cgagguuaaa uucaagcgcu ga                        3642


<210>  4043
<211>  3651
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4043
augaccaaga uccugggcau ugacacaggg acgaacagcc ucggaugggc caucguggau       60

aaaaccgcug acaauuacaa ccugcuugau aagggcguca auauuuucca ggaggggguu      120

aaaaucgaaa agggaauuga guccucuaaa gccgcggaaa gaacagagca ccgggcuacc      180

cgcgugagau auuaccggau caagcugcgc aagaucagac uccugcggau ucuuagcgac      240

aaccaucugu gucccccucu cucccaagaa gaacugucua cauggcgccu gaaaaaggug      300

uauccgaaaa augagcucuu uaugcagugg cagagaaccg augacgauac agaaaagacc      360

cccuacgccu aucggcacaa augcauccau gagaagcugg accuuagcga ugucacggac      420

cgcuacauuc ugggcagagc cuucuaucac aucauucagc ggcgcggguu ucucuccaac      480

agaaaggaac aaggaggcga ggauucuggc aaagugaagg aggggaucag cacccugaca      540

caugaaauga augaggcugg auacgaauac cuuggcgacu auuucuacgc ccuguauaac      600

aaaggggaga agauucggaa acacuacacc gcucgcaaug aacacuuucu cuccgaguuc      660

aaggccaucu gcgagaaaca ggaucuggaa aaggagcugg gaagcgaagu ugugagaaac      720

aucgagaagg cgauuuuuga ccagcggccu cucaaaucuc agaagggcca agucgggaaa      780

uguguguucg aaaagaauaa aacaaagugc cccuccagcc auccuaugua ugaggaguuu      840

agaaugcuga ccuucaucaa caauauuaag aucaaaacgc ccaacgacga uguucuucgc      900

ccucugaaua caggagaacg ggagaagauu augccgcucu uuuucagaaa aucuaagaaa      960

caguuugacu ucgaagauau caccaagaag cuggccccca aaaagaugua cggcuucuau     1020

aaaaaggagg aagacgcuga gaugccuuuu cuuuucaacu accccaugga uacauccgug     1080

agcggcuguc cugucaccgc cgcucugaaa gacguguuug gggaugacug gaucaccgcc     1140

auuugcgaga cauacacgcu cggagaaggc aagucucgcu ucgauaucgu gaacgacauu     1200

uggcacgcgc uguauuuuua cuccgauaag gacaaacugg ccgaguucgc uaucaaucgg     1260

cuccagcuga gcgaagacga ggccaagauc uuuuccgaga uuucucuucc cgcugauuau     1320

gccagccugu cccucaaagc caucuguaag auucugccgu accuuagacg cgggcugauc     1380

uauagccaug cugucuuccu cggaaaccug ugcgaaguua ugccuaaaua cgaguggucu     1440

auuaaggaaa ugcgggagac caucauugac aauaucauua uggaaaugga uaaaugugac     1500

acaaaggaua agagaaccuu ugaggugugc aucaaagacu aucugaaggg ccaguacaac     1560

guguccgaua auagccucaa aaagcuguac caccccucua ugaucgagac auauccucgc     1620

guccaauaca ccaacgaaca cgggguguau cagcuuggaa gcccccggau ugacuccguu     1680

agaaauccca uggcgaugcg cucuauguuc cggcugagaa aacucgugaa ccggcugcug     1740

gucgagggca agaucgauca gaauacggaa gugcauauug aguuugcccg cgaacuuaac     1800

gacgcuaaua agagaaacgc caucgcccuc uucguuaaag agaaucaaag cgcuaacgau     1860

gaggcccgga agcagauuau cacacuguuu aaaaaggaaa ccggcaccga caucgagccu     1920

acagauaaag acauucugaa guacgugcuc ugggaggagc agaaucacau cugucuguau     1980

accgggaagc agauuagaau cuccgacuuc auuggagcga acccgaaauu cgauaucgaa     2040

cauacaaucc cccgcagcgu cggcggggac ucuacgaaga ugaaccuuac ccugugcgau     2100

ucccgguuua auagagaggu gaaaaagaca aaacucccug ucgagcugcc caaccacgac     2160

gaaauuaugg agcgcaucaa ggaauggaag aaaaauuacg agcugcuuga uauucaaauc     2220

agaaagaaca aaaagcucag caaaggagcu accacaaagg agcagaaaga caccauuauc     2280

cggaagcgcc aucugcugga acucagacgg gauuauuggc gcggcaagua cgagagauuc     2340

acgauggaau cugugccuga gggguuuucc cggcgccagg gcaccgacau cagcguuauu     2400

uccaaauacg ccagacugua ucuuaagucu cuguucaauc ggguguacac agucaaagga     2460

aucgccacca gcgauuuucg caagauuugg ggcauccagg acauuuauuc caaaaaggaa     2520

agaaucaacc acgugcacca uugcauugau gcuaucguua ucgccuguau ugggcucgac     2580

gaguacaaua agcugggagc uuauuaccac gaugaggaaa acaauaaaug guauggcaug     2640

ucuaaggcca gcuucaaaaa gcccugggcg acauuugugg aggacaucaa aaagauucaa     2700

gacgaaaucc uggucuacca uuacaccccg gauaacaugc cuaagcaggg gcggagacgc     2760

auuaaaaucg acggaaagaa aauccuuugc aagggcgaug ccgcucgggg gucccuccac     2820

aaugacacgu auuacggcgc cauugagaac gauggagcug ugaaauaugu gaagagaauc     2880

gaccuggcca gccuggaaga gaaggauguu aaaaauauug ucgacgauac agugcgcucu     2940

aucauugagg cggccaucaa ggaaaaaggc uucaaggacg cuauggccuc caccaucugg     3000

augaacgagg aaaaacgggu gcccauuaag aaggucagau guuuuacacc uagcaucacc     3060

aaaccccuca auauucgcaa gcagcgggau gugucuauca aagaguacaa gcagcaauau     3120

cacguugcua acgacuccaa cuaccugcuu gcccuguaua uugggaccga caauaaagga     3180

aaggaaaaga gagaguucga gaucgugaac auucuccagg ccgcucagua cuaccgcaca     3240

agcaaugaua aagaagucgu ggaccggcau aucguuccua ucaagucuga gcacgauuau     3300

cccuuugcgu acacgcugaa aauuggcacc auggugcugc uuuaugaaaa gagcccgaac     3360

gaggucuggg acgccacaau caaagagaga aaucggcgcc ucuacaaggu gaccgggcug     3420

uccacaauga gaaugaaagg acggaacggc gaauaugcuu acgccaccgu uaagcugauu     3480

cauaaugagg aagcccgccc uagcaaggau aucaaagcua agaacgggga guaugaacag     3540

ggcgaggagu ucagacccgc cauuaucaug cuccacacgc aacugaaugc gcuugugcag     3600

ggauacgacu uugaaaucaa cgagcugggc gaaauucgga gacuccgcug a              3651


<210>  4044
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4044
auggagaagc agagaauccu gggccucgac cccgggacca acagccuggg augggccgug       60

cuugauuucg acaauuccaa acaagaauac aaccuggucc ggaagggcga gguuauuuuu      120

gaagaggggg ugggaaaauc ugaacgcggc gaguucagca aggcugccua uagaacauuu      180

uacaaaucca cgcggaagca cuauuuccgc agacggcucc gcaagaucga ggugcugaaa      240

guccuuguga aguacgauuu uugcccuuau cugucugacg aucuccugaa acaguggcau      300

gaaaagaaaa uuuacccggc gguuaaggag uucauggacu ggcagagaac cagcgaugcu      360

gaagggaaga aucccuauua cgaccggcac uguugccuga acgagaaacu cgaucugucc      420

cuuaagucug accgcuacau ccugggcaga gcccucuauc aucugacaca gcggcgcgga      480

uuuaaaagca auagacuuau ucaaaacaau gaugagaacg aagagggcaa ggugaaaaag      540

gggaucuccg aacugagcac cgagaugcag gaagccaauu guaacuaccu cggagacuau      600

uucuacaaac uguauacaga gaagggcaau accguccaga uccggaagcg cuacacagau      660

agagagaaac acuauaagaa cgaauuuaau gcuauuugcg agaaacagga acugaacgag      720

accaucguga aggaacucga caaagccauu uucuuucaac ggaagcugaa gccucagaga      780

caugggguug gaaaauguac guucgagccc aauaaccgcc ggugcgcuau cucucacccu      840

gacuuugaga gauuccgcau gcuuuguuuc auuaacaaua ucaagauuaa aacccccuuu      900

gaagagaacc ugcggccucu caauaacgaa gagaaggccc ugaucgaacc gcuuuucuac      960

agaaaaucca ucaagaauuu ugauuucgag gagauugcga agaaacuggc cggcaagaac     1020

aauuacugcu auaucaaaga cgauagcucu aagcaguaca aauuuaacua ucgcaugucc     1080

cagggggcua gcacauguca aaccauugcc aagcucaagu cuaucuucgg agaagagugg     1140

aaaaauggca uugcugaaac auacaccaac cggagaaaga aagagggccu gaagaaacag     1200

gccgagaugg uggacgauau cuggaauguc cuguauuccg ugagcgaaug cgacgugcuc     1260

aaggauuuug cgaucaacaa acugcagcuu gacgagaaga cggccgauaa uuucuccaag     1320

acaaaacugg cucaaggguu ugccacccuc ucucugaagg cuauucgcaa aauccuuccc     1380

uuccuggaac agggauuugg cuacacagag gccgucuucc ucgccaacau uccuaccguu     1440

guggggaagg aauauuggaa uaacgaggac auccggaacg auauuaugga ggaaaucaaa     1500

accaucuuuu accacaauac acugaagacg aacgacagcc auaauaccau ucuguucugu     1560

aucaaggaca uucucuccaa caauuucgau cuggcucccg gagcgaucga caaacuuuac     1620

cacccuagca ugauuaacgu guauaaggau gucaucccca augaggacgg cauucugcag     1680

cucgggucuc cgaaaaucga ugccgugaag aacccuaugg cuaugagauc ccugcauauc     1740

auucgcaaag uugugaauga acugcuucgg gagggaauca uuaacagcga cacagaaguc     1800

agaaucgagu acgcccggga gcucaaugau gccaacaagc gccacgcuau ugccgacugg     1860

cagaagaaac aauauggcaa gcaccagaaa uacagaaagg aaaucaaaga gcuguuuaag     1920

gcggaaaccg gcuucgagau cgaacccaau gagacagaua uucugaaguu ugagcucugg     1980

gaggaacaga accauaucug ccuguauacc gggcaggaaa uuggaaucuc ugaguucgug     2040

ggcgagaauc cuaaauacga cauugaacac acguuucccc ggagccuugg gggagauucc     2100

acaaagauga acaugacccu gugcgagaac aaauucaaua gagaaaacaa gcagaaaaag     2160

cuccccucug agcuggcuaa ucauagcgaa auccuggagc gcaucgacga uuggaaaaag     2220

aagauuuaua uccuucagaa aaaguacgac uccauucgga cccacaacgg cauggacaaa     2280

gagaauaagg auaaaaagau cguuagcaga cauaagauua aacucgaacu ggacuacugg     2340

aaggggaaau auaaccgcuu uacaaugacc gagaucccug aaggcuucuc uagacggcag     2400

ggaaugggca ucgggcugau uucccgcuac gccggacucu aucugaagac aaucuuucac     2460

aaaaaggaua auccgcaaaa gagcaacgug uacauuguca aaggcucuau cacggccgag     2520

uucagacgga uuuggaauau ccaagacgau uaugaaaaga aaugucgcga caaccacauu     2580

caucacugca ucgaugcuau cguggucggg uguauuggaa agagagagua cgaccuuaug     2640

gcccaguuuu aucgggauga ggaaaaauac cgcugggaga agaagaaaaa gcccuucuuu     2700

aaaaagccuu gggaaaccuu cacacaggac augcuguccc ucaaagauga ggugcugacc     2760

guucauuuca aucccagcaa cuuuacaaag aaggcucaca aaaagguguu caccccuaaa     2820

ggcaucuuug ucgcccaggg ggacugcgcg agagugaagc ugcacaaagg cuccuacuuc     2880

ggagccauug aacaaaaggg cgagaucaaa uauguuaugc ggaaggagcu uucugcucuc     2940

aagauugaag auaucaaaaa uaucguggac gccgucguga aggagaaagu gcuggcuguu     3000

gucaagaaaa agggguuuaa gcaggccaug gcggaaccca uuuacaugaa cgaggacaaa     3060

cgcauccuga uuaagaaagu gagacucuuc gugagccagg ccaauccgcu gaucgucaag     3120

aaacaucggg aucuuuccuc uaaggaguau aagcaaaacu acuaugugga caacgaagga     3180

aaucugauga uugcuaugua cgagggcguu aaaaagaacg ggaaaaucga uagagaaauc     3240

acggugguca auaaccucga ggccgcuaag uuuuuccgcc agagccagaa aaauaacgcc     3300

gaaaagcagc ugauuuccca ccugagcccu aagaauggau auccccuuaa aaccgugcuc     3360

acacaaaagc agcugguucu gauguacgag gacucuccua aagagaucaa gcuccgggau     3420

accaaaaaca uggugaagag acuguaccag gucuuuggca uugaaaaaga cgggcgcgug     3480

aaacuuaagu uccaucagga ggcccggucc gaaggccuga auaucaacag cgcugcguuu     3540

aagauucaag auacacccga gucucucuau agacacacca aguccaaucu gaaaguucug     3600

gugaacggag ucgacuucaa gaucaauauu cuuggcgaaa ucagccugau cuga           3654


<210>  4045
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4045
auggagaagc agagaauccu gggccucgac cccgggacca acagccuggg augggccgug       60

cuugauuucg acaauuccaa acaagaauac aaccuggucc ggaagggcga gguuauuuuu      120

gaagaggggg ugggaaaauc ugaacgcggc gaguucagca aggcugccua uagaacauuu      180

uacaaaucca cgcggaagca cuauuuccgc agacggcucc gcaagaucga ggugcugaaa      240

guccuuguga aguacgauuu uugcccuuau cugucugacg aucuccugaa acaguggcau      300

gaaaagaaaa uuuacccggc gguuaaggag uucauggacu ggcagagaac cagcgaugcu      360

gaagggaaga aucccuauua cgaccggcac uguugccuga acgagaaacu cgaucugucc      420

cuuaagucug accgcuacgu gcugggcaga gcccucuauc aucugacaca gcggcgcgga      480

uuuaaaagca auagacuuau ccaaaacaau gaugagaacg aagagggcaa ggucaaaaag      540

gggauuuccg aacugagcac cgagaugcag gaagccaauu guaacuaccu cggagacuau      600

uucuacaaac uguauacaga gaagggcaau accgugcaga uccggaagcg cuacacagau      660

agagagaaac acuauaagaa cgaauuuaau gcuaucugcg agaaacagga acugaacgag      720

accauuguua aggaacucga caaagccauc uucuuucaac ggaagcugaa gccucagaga      780

cauggggugg gaaaauguac guucgagccc aauaaccgcc ggugcgcuau uucucacccu      840

gacuuugaga gauuccgcau gcuuuguuuc aucaacaaua uuaagaucaa aacccccuuu      900

gaagagaacc ugcggccucu caauaacgaa gagaaggccc ugauugaacc gcuuuucuac      960

agaaaaucca ucaagaauuu ugauuucgag gagaucgcga agaaacuggc cggcaagaac     1020

aauuacugcu auauuaaaga cgauagcucu aagcaguaca aauuuaacua ucgcaugucc     1080

cagggggcua gcacauguca aaccaucgcc aagcucaagu cuauuuucgg agaagagugg     1140

aaaaauggca ucgcugaaac auacaccaac cggagaaaga aagagggccu gaagaaucag     1200

gccgagaugg ucgacgauau uuggaacgug cuguauuccg ugagcgaaug cgacguccuc     1260

aaagauuuug cgaucaauaa gcugcagcuu gacgagaaaa cggccgauaa cuucuccaag     1320

acaaagcugg cucaaggguu ugccacccuc ucucugaaag cuauccgcaa gauucuuccc     1380

uuccuggaac agggauuugg cuacacagag gccguuuucc ucgccaauau cccuaccgug     1440

guggggaaag aauauuggaa caacgaggac auucggaaug auaucaugga ggaaauuaag     1500

accaucuuuu accacaacac acugaaaacg aaugacagcc auaacaccau ccuguucugu     1560

auuaaggaca uccucuccaa uaacuucgau cuggcucccg gagcgauuga caaacuuuac     1620

cacccuagca ugaucaaugu cuauaaagau gugauuccca acgaggacgg cauccugcag     1680

cucgggucuc cgaagauuga ugccguuaaa aauccuaugg cuaugagauc ccugcauauc     1740

auccgcaagg uggucaacga acugcuucgg gagggaauua ucaauagcga cacagaagug     1800

agaauugagu acgcccggga gcucaacgau gccaauaaac gccacgcuau cgccgacugg     1860

cagaagaagc aauauggcaa acaccagaag uacagaaaag aaauuaagga gcuguuuaaa     1920

gcggaaaccg gcuucgagau cgaacccaac gagacagaua uccugaaguu ugagcucugg     1980

gaagaacaga aucauauuug ccuguauacc gggcaggaaa ucggaauuuc ugaguucguu     2040

ggcgagaacc cuaaguacga caucgaacac acgauucccc ggagccuugg gggagauucc     2100

acaaaaauga acaugacccu gugcgagaau aaguuuaaca gagaaaauaa acaaaagaaa     2160

cuccccucug agcuggcuaa ccauagcgaa auccuggagc gcaucgacga uuggaagaaa     2220

aagauuuaua uccuucagaa gaaauacgac uccauucgga cccacaaugg cauggacaag     2280

gagaacaaag auaagaaaau cgugagcaga cauaagauua agcucgaacu ggacuacugg     2340

aaagggaagu auaaucgcuu cacaaugacc gagaucccug aaggcuuuuc uagacggcag     2400

ggaaugggca ucgggcugau uucccgcuac gccggacucu aucugaaaac aaucuuccac     2460

aagaaagaua acccgcaaaa gagcaauguc uacauuguga agggcucuau cacggccgag     2520

uuuagacgga uuuggaacau ccaggacgau uaugagaaaa agugucgcga caaucacauu     2580

caucacugca ucgaugcuau cgucgugggg uguauuggaa aaagagagua cgaccuuaug     2640

gcccaguucu aucgggauga ggaaaaguac cgcugggaga aaaagaagaa acccuuuuuc     2700

aagaaaccuu gggaaaccuu uacacaggac augcuguccc ucaaggauga gguucugacc     2760

gugcauuuca accccagcaa uuucacaaag aaggcucaca agaaagucuu uaccccuaag     2820

ggcaucuucg uggcccaggg ggacugcgcg agaguuaaac ugcacaaggg cuccuacuuu     2880

ggagccauug aacaaaaagg cgagaucaag uaugugaugc ggaaaaagcu cucugcucuu     2940

aagauugagg auaucgaaca uaucgucgac gccgugguga aagagaaggu ucuggcuguc     3000

gugaaaaaga aaggguucaa gcaggccaug gcggaaccca uuuacaugaa cgaggacaag     3060

cgcauccuga uuaaaaaggu gagacucuuu gucagccaga cgaauccgcu gaucgugaaa     3120

aagcaccggg aucuuuccuc uaaagaguau aagcaaaacu acuauguuga caacgaagga     3180

aaucugauga uugccaugua cgagggcgug aagaaaaacg ggaagaucga uagagaaauc     3240

accgucguga auaaccucga ggcugccaaa uucuuucgcc agagccagaa gaauaacgcu     3300

gaaaaacagc ugauuuccca ucugagcccu aagaauggau auccccuuaa gacaguucuc     3360

acccaaaaac agcuggugcu gauguacgag gacucuccua aggagaucaa acuccgggau     3420

acaaagaaca uggucaaaag acuguaccag guguucggca uugaaaagga cgggcgcguu     3480

gagcuuaaau uucaccagga agcccggucc gagggccuga auaagaacag cgccgcuuuc     3540

aagauccaag auacccccga aucucucuau agacauacga aauccaaucu gaaggugcug     3600

gucaacggag uggacuuuaa aauuaauauc cuuggcgaga uuagccugau cuga           3654


<210>  4046
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4046
augaaguaca acuaugugcu gggccucgac aucgggauua gcuccgucgg auggggccug       60

cuugcccugg augagaauaa caaucccuac aaaaucauug acguuggguc uagaaucuuc      120

agcccuggag aaguggagaa gaccggcgau uccaaagcua aggaacggcg cgagaaaaga      180

gggacacggc gcgugauuag acggcgcgaa uuuagacucg accggguccg caaccugcuu      240

uaugagaagg gauaccugaa gggcaauauc acgucuaaca ucgugagcgu uaaaaaugag      300

gaacucacca uuaucuucaa cgauauggug aauaacuauu acaaguccaa agacacaaau      360

ccguauaagc ugaaagucga ggcccuggau agaaagcuca ccaacgacga acugucuauu      420

auccuugugc acuacgcgaa gaaacggggc uacaagagca aucgcgagga guccagcgau      480

aacgacaaug ggaaaguuaa ggaagcuauu aaagagaaca caaaccugau ggaagagaag      540

aaauauagaa ccaucucuga aauguacauu aagaaugaga aguuuaaaaa caagaucaaa      600

aauggacccg augacuauaa gcucuccgug acaaacgaga uguaccugga agagaucaau      660

aaaguccuug auagccagau uggcuucggg cugaucgacg acaacuuuaa gaaugaauau      720

cucgagauuu acaacucucg gcgccauuau uccgaaggac cuggcuacua cuaugccaag      780

gaugccaaug ggaacaaagu gaaggugaga agccccuacg gaggcgaccu gaucaaaaag      840

augaccggcg ucugcucuuu cgauaaaaag gcucgggccc cuaaguaugc uuuuuccagc      900

gagcuguucg uuuccauuuc uaaacucgug aaucugagau acaagaucga cgaugacagc      960

uauaaacaac uuacgaagga gcagaucaac gaacugauug augaggccaa aaauaagaac     1020

aaggugaccu acaaaucccu caucaagauu cuggggauca aaaauauuaa ggucaaagac     1080

cuuaaccuga gcaagucuga auauaucaaa gugaucgaug agcucaagaa gaaacugaac     1140

auuuccaagg aagagaaaau cgacauuuac agccuggaug aggaaaauaa gaaaaucuac     1200

gacgacauuu auaacgagaa gcucuuuucu aagacacuga ucgaguucaa aggauacaau     1260

cuucugaaga aagcgauuac ccucagcuau aacuccggca cauggaauga uguuaaggac     1320

aacaucgaau uucuggauga gcuugcccug uacuguaccg acuauaaagu gaauaacgaa     1380

auccaggagc acauucauaa uucucucauc auugauagcc gcuucgacga uuccaacuuu     1440

gucgaaaauc ugcccaacuu caaggaccac cugaugcuca gcacggauau cauucggaag     1500

cugaucccua ucaugcuuga ggggauuaca uacgacaaag cuauggauaa gaucggauau     1560

aaucauucua accuguacaa agacaccauu aaggaagauc uccuggugcc gguuuacgug     1620

uccaaaaaua ucacaaacca gagagucauu cgcagccuga cccaagcccg gaaggugauc     1680

aacgcuauca uuaagaaaua uggcucuccc aagaucauua auaucgagac cgccagagag     1740

cuugcgaaaa cacagaagga acgccggauu aucgagaaaa accagcagga caagcaauac     1800

gaaaaugaga gaaucaaaaa gcaccucguc gaacuggagc uguucuccga cgagagcaag     1860

auuacgggga acgaucuccu gaaauauaaa cuuuggcagg aacagaaaga guuuugcgga     1920

uacuccauga agaaaaucaa gcuggaagac cucuucucua auaacauugu gcagaucgau     1980

cacauucugc cuuauagccg caccuacaau gacaacuauc ugaauaagac acuuguuuac     2040

accaaauaca accaagauaa gggcaauaaa acacccuaug agugguuugg caagaccgac     2100

aaauggcagg aauacgaggc cuucaucaac ucccucucua uuagccagaa uaagaaggau     2160

aacuaucugc ugaaaaaucu cgaccaagau gcugagcggg aaaugagaga gcagaaccug     2220

aacgacacga aguacaucuc ccgcgaacuu cugagccuca ucaaagccua ucugaaugug     2280

gaugagguca acgcuuuucc uggggccauu acagccaagc ugcgggcuag auggggauuc     2340

aaucggcuua cccauucuua caucuccaaa accuauauua ugcccgagga caugaagaag     2400

ggcaucaaca aagaucgcga caaucaccuc caucacgcga uggacgcccu ggugauugcu     2460

agcaucacaa agucucugca gcagaaaauc acccucuacg aaaaguuuuc cagauacguu     2520

gaugggauua caaagaacga gcugggaaag aucagcacgg gcaauauuaa cgacgaucuu     2580

uauaauuacu augacgaaga gaccggggaa aucaaggagc uggagcuccg gcaauacauu     2640

aaaaaccagg uggcccagaa uaacaucuau uucucuaagc acaaugucag caaacugauc     2700

uuuccgacac cuuacaacaa uuucguggaa gaggccaagc ugagagugua ugaacgcgau     2760

cuugagguuc ucaaauggaa ccugaagaau cugggcaccu acacauccag cgaacucaac     2820

cggauucaug uccugauccc cucuguggcu aaaaacaaga uuuccggaag acuucacgag     2880

gagaccuacu auggccugaa ggaagugggg aucggagagg acaaagucac guacaagacc     2940

cucaaaacac cuauuagcaa ggugaaaaag aaggaucugg acaauauccc cgauuguaaa     3000

ggcgggucca aggacauuua uaacgcccug guuaaauggu uuggaaauug cgaaaccggc     3060

gaggaagcgc uuaagaugca ugaugggaaa uaccccguga acccuaauga caaggagcag     3120

aaggagaucc gcaaaaucaa ggucuauaca aaauacaacg cuaccggcca cgugguuaau     3180

aacggaaaug uggaaaaggg cgggauuuau agaaucgacg ucuucaaauc uaagaacaag     3240

gaggauaaua aacuguacuu uacgagcuac gacauuuucg aaaucgagca aauuaaggcc     3300

uaugagaaag gaaagaacau caauuucucc aucaaacucg aauacggcca ggggaagaac     3360

aauaaaauuc ugcucuauaa ggaugugaag aacgaguacg accugaucau gucuauuuau     3420

aaaaacgauc uuauccggau uaagacaaaa accggaaaua agagcacagc cuacguugug     3480

ggcuuuuccg ggggcaaacu ggaaaucaag ucuaccaucg gagacggcua ugauauuauc     3540

gggaacaaua acauuuucga caagagcaug gagcgcuaca gagucaccuc cagcacaauc     3600

gugaaaauug auaagcucuc uaucuccauu cugggagaag ugagcggccu guga           3654


<210>  4047
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4047
augaacaaga gaauccuggg ccucgacacc gggacaaaua gccugggaug ggccgugguc       60

gauugggacg agcacgcuca guccuacgaa cuuauuaaau auggcgaugu uaucuuccaa      120

gaggggguga agauugaaaa aggaaucgag ucuagcaagg ccgcggaacg guccggcuac      180

aaagcuauuc gcaagcagua uuuuagacgg cgccugagaa agauccaggu gcucaaaguc      240

cuggugaagu accaucuuug ccccuaucug ucugacgaug accuccggca guggcaucug      300

caaaaacagu acccuaagag cgaugagcug augcucuggc agcgcacguc cgacgaggaa      360

gggaaaaacc cguauuacga uagacaccgg ugucugcacg agaagcugga ccugaccguu      420

gaagccgauc gcuacacacu cgggagagcc cuguaucauc uuacccagcg gcgcggcuuc      480

cugucuaaua gacucgacac aagcgcugau aacaaggagg acggcguggu caaauccggg      540

aucagccaac ugucuaccga gauggaagag gccggaugcg aauaccuggg cgacuauuuu      600

uacaagcucu aucacgagca ggggaauaaa gugcggauuc gccagagaua cacagaucgg      660

aagaaacacu aucagcauga guucgacgcu aucugugaaa agcaagagcu guccagcgaa      720

cuuauugagg aucugcagag agccaucuuu uuccagcucc cccugaaauc ucaacgccac      780

ggaguuggcc ggugcaccuu ugaaagaggg aagccucgcu gugcggacuc ccaucccgau      840

uacgaggagu uccggaugcu uugcuuugug aacaauauuc aggucaaggg accucacgac      900

cuggaacuca gaccccugac guacgaggaa cgcgagaaaa ucgaacaccu guucuuuaga      960

aagagcaaac cuaacuucga uuuugaggac auugccaagg aacucgcugg caaaaagaau     1020

uaugccugga uccaugauaa ggaggagcgg gcuuacaaau ucaacuaucg caugacccag     1080

ggcgugccgg ggugucccac aaccgcccag cugaagucua ucuucggaga cgauuggaaa     1140

acaggcauug cggaaaccua cacgcuuauc caaaagaaaa auggguccaa gagccugcag     1200

gagauggugg acgacgucug gaacguucuc uauuccuuuu cuagcgugga aaagcugaaa     1260

gaguucgccc accauaaacu ucagcuggau gaggaaaaug cugagaaauu ugccaagauu     1320

aaacucuccc acagcuucgc ugcccugucu cugaaggcca uccggaaguu ucucccuuuc     1380

cugagaaaag gaauguacua uacacaugcu uccuuuuucg cgaacauucc caccaucgug     1440

ggcaaggaaa ucuggaauaa cgagcagagc cgcaaauaca uuauggaaaa cgucggggag     1500

cuuguguuua auuaccaacc uaagcaccgg gacguucagg gaacaaucga gaugcugauu     1560

aaagauuucc ucgccaacaa uuuugaacug cccgcuggcg ccaccgacaa gcuguaucac     1620

ccgucuauga ucgagaccua cccucaugcc cuuagaaacg aguucggcau ucuccagcug     1680

gggagccccc ggacaaaugc uauccgcaac ccuauggcca ugagaucccu gcacgugcuc     1740

cggagagucg ugaaucagcu gcuuaaagaa ucuaucauug augaaaacac ggagguucau     1800

guggaguaug cgcgcgaacu gaaugacgcu aacaagcgga gagccaucgc cgaucgccaa     1860

aaggagcagg acaaacagca caagaaauac ggagaugaaa uuagaaagcu cuauaaagag     1920

gaaaccggca aggacaucga gcccacacag accgaugucc ugaaguuuca acugugggag     1980

gaacagaauc acuacugccu uuauacaggg gagcagauug gaaucaccga cuucauuggc     2040

agcaacccca aauucgauau cgaacauacg aucccucaau ccgugggagg cgacagcaca     2100

cagaugaauc ucacccugug ugacaaccgg uuuaaucgcg aggucaagaa agcuaagcug     2160

ccgaccgagc ucgccaacca cgaagagauu cugacaagaa ucgaacccug gaaaaacaag     2220

uacgagcagc uugugaagga acgggauaaa cagcgcaccu ucgcuggaau ggacaaggcc     2280

guuaaagaua uuagaaucca aaagcggcau aaacugcaga uggagauuga cuacuggcgc     2340

ggcaaguaug agagauuuac aaugacggaa gugccugagg gguucucucg gcgccaggga     2400

accggcaucg ggcucaucuc cagauacgcg ggacuguauc uuaagagccu uuuucaccag     2460

gccgauucuc ggaauaaauc caacgucuac gugguuaagg gcguggcuac agccgaguuc     2520

agaaaaaugu gggggcucca aagcgaauau gaaaagaaau gccgcgacaa ucauucccac     2580

cacuguaugg augcuauuac caucgccugc auuggcaagc gggaguacga ccugauggcg     2640

gaguauuacc auauggaaga gacauuuaga cagggacgcg gcucuaaacc caaguucagc     2700

aagccuuggg ccaccuuuac ggaagauguc cugaacaucu acaaaaaucu ccugguggug     2760

cacgacaccc ccaacaauau gccgaagcau acaaagaagu auguucagac cuccaaaggg     2820

aagguccuug cucagggaga uacagcccgg ggcucucugc accucgacac cuacuauggg     2880

gcuauugaga gagauggaga aauccgcuac guggugcgga gaccucugag cuccuucacg     2940

aaccccaagg agcuggagaa uaucgucgac gaaacaguga aacgcaccau uaaggaggcc     3000

aucgccgaca aaaacuuuaa gcaagcuauu gcggaaccua ucuauaugaa ugaggauaaa     3060

ggcauucuua ucaagaaggu ucggugcuuc gccaacagcg ugaaacagcc caucaauauu     3120

agacagcauc gggaccucuc uaagaaagag uacaagcaac aguaucacgu caugaacgaa     3180

aauaacuacc ugcuggcuau cuacgagggg cucgugaaaa acaagguugu gcgcgaauuu     3240

gagauugucu ccuauaucga agccgccaag uacuauaaaa gaagccagga ucggaauauu     3300

uucucuucca ucgugccuac acacagcacc aaguacggcc ugccccuuaa aaccaagcug     3360

cucaugggac agcaaguucu gauguucgag gagaacccgg acgaaauuca gguggauaau     3420

acaaaagacc uggucaagcg ccuuuauaag guggugggca ucgagaaaga ugggagaauc     3480

aaguuuaaac aucaccagga agcucggaag gagggacuga cgauuuacuc uaccccuuau     3540

aaaaacaaug acgauuacgu ccccaucuuc agacagagca uuaagaacau caauauucuc     3600

guugacggca ucgauuuuaa caucgacauu cuggggaaag ugacacucaa ggaauga        3657


<210>  4048
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4048
augaacaaga gaauccuggg ccucgacacc gggacaaaua gccugggaug ggccgugguc       60

gauugggacg agcacgcuca guccuacgaa cuuauuaaau auggcgaugu uaucuuccaa      120

gaggggguga agauugaaaa aggaaucgag ucuagcaagg ccgcggaacg guccggcuac      180

aaagcuauuc gcaagcagua uuuuagacgg cgccugagaa agauccaggu gcucaaaguc      240

cuggugaagu accaucuuug ccccuaucug ucugacgaug accuccggca guggcaccuu      300

caaaaacagu acccuaagag cgaugagcug augcucuggc agcgcacguc cgacgaggaa      360

gggaaaaacc cguauuacga uagacaucgg ugucugcacg agaagcugga ccugaccguu      420

gaagccgauc gcuacacacu cggcagagcc cuguaucauc uuacccagcg gcgcggauuc      480

cugucuaaua gacucgacac aagcgcugau aacaaggagg acggcguggu caaauccggg      540

aucagccaac ugucuaccga gauggaagag gccggaugcg aauaccuggg cgacuauuuu      600

uacaagcucu augaugcuca ggggaauaaa gugcggauuc gccagagaua cacagaccgg      660

aacaagcacu aucagcacga guucgaugcc aucugugaaa aacaagagcu guccagcgag      720

cuuauugaag accugcagag agcgaucuuu uuccagcucc cccugaaguc ucaacgccau      780

ggaguuggcc ggugcaccuu ugagagaggg aaaccucgcu gugccgauuc ccaccccgac      840

uacgaagagu uccggaugcu uugcuuugug aauaacauuc aggucaaggg accucaugau      900

cuggaacuca gaccccugac guacgaggag cgcgaaaaga ucgagccucu guucuuucgg      960

aaaagcaagc cgaauuucga cuuugaagau auugcuaaag cccucgcugg caagaaaaac     1020

uaugccugga uccacgacaa ggaggaaaga gcguacaagu ucaauuaucg caugacccag     1080

ggcgugcccg gguguccuac aaucgcccag cugaaaucua uuuucggaga ugacuggaag     1140

accggcaucg cugagacaua cacccuuauu caaaagaaga acggguccaa aagccugcag     1200

gagauggugg acgaugucug gaauguucuc uauuccuuuu cuagcgugga aaagcugaag     1260

gaguucgccc accauaaacu ucagcuggac gaagaguccg cugagaaguu ugccaaaauc     1320

aagcucagcc acucuuucgc cgcucugucc cugaaagcga uucggaaguu ucuccccuuc     1380

cugagaaagg gaauguacua uacgcaugcc agcuuuuucg cuaacauccc uacaaucgug     1440

ggcaaagaaa uuuggaauaa ggagcagaac cgcaaauaca ucauggaaaa cgucggggag     1500

cuuguguuua auuaccaacc caagcaccgg gaaguucagg gaaccauuga gaugcugauc     1560

aaagauuucc ucgccaacaa uuuugagcug ccggccggcg cuacagacaa gcuguaucau     1620

ccuucuauga uugaaaccua ccccaacgcc cagagaaaug aguucggcau ccuucagcuc     1680

gggagcccuc ggaccaacgc gauccgcaau cccauggcua ugagaucccu gcacauucug     1740

cggagagugg ucaaccaacu ccugaaagaa ucuaucauug augagaauac agaagugcac     1800

guugaguaug cccgcgagcu uaacgacgcc aauaagcgga gagcuaucgc cgaucgccag     1860

aaggaacagg acaaacagca uaagaaauac ggagaugaga uuagaaagcu guauaaagaa     1920

gagacgggca aggacaucga acccacccaa acagaugugc ucaaguuuca gcugugggag     1980

gagcagaacc accauugccu guacaccggg gaacaaauug gaaucacaga cuucaucggc     2040

agcaauccua aauucgauau ugagcacacc aucccgcagu ccgucggggg cgacagcacg     2100

cagaugaacc uuacacucug ugacaaucgg uuuaaccgcg aagugaagaa agcuaagcug     2160

cccaccgagc uggccaacca cgaggaaauu cucaccagaa ucgagccuug gaaaaauaag     2220

uaugaacagc uggucaagga gcgggauaaa caacgcacau ucgcgggaau ggacaaggcc     2280

gugaaagaua uuagaaucca gaagcggcau aaacuucaga uggaaaucga cuacuggcgc     2340

ggcaaguacg agagauuuac caugacagag guucccgaag gguucucucg gcgccaggga     2400

acgggcauug ggcugaucuc cagauaugcu ggacucuacc ugaaaagccu guuucaccaa     2460

gccgauucuc ggaacaaauc caauguguau gucgugaagg gcguugcuac cgccgaguuc     2520

agaaaaaugu gggggcuuca gagcgaauac gagaagaaau gccgcgacaa ccauucccac     2580

cauuguaugg augcgauuac aaucgccugc auuggcaagc gggaauauga ccucauggcu     2640

gaguacuaua gaauggagga aaccuuuaaa cagggacgcg gcucuaagcc uaaguucagc     2700

aaacccuggg ccacauuuac cgaggaugug cugaauaucu acaagaaccu gcucgucgug     2760

cacgacacgc cgaauaacau gccuaaacac accaagaaau acgugcagac auccaucggg     2820

aagguucugg cucaaggaga uaccgcccgg ggcucucuuc aucuggacac auauuacggg     2880

gccauugaaa gagauggaga gauccgcuau gucgugcgga gaccccucag cuccuucacc     2940

aagccugagg aacuggagaa uauuguggac gaaacgguca aacgcacaau caaggaggcu     3000

auugcggaca aaaacuuuaa gcaggccauc gcugaaccca ucuacaugaa ugaggagaaa     3060

ggcauucuga ucaagaaggu gcggugcuuc gccaaaagcg uuaagcagcc uauuaacauc     3120

agacaacacc gggaucuuuc uaaaaaggaa uauaaacagc aguaccaugu gaugaaugag     3180

aacaauuauc uccuggccau uuacgaaggg cuggucaaga acaagguggu ucgcgaguuu     3240

gaaaucgugu ccuacauuga ggcugccaaa uauuacaaga gaagccagga ccggaacauc     3300

uucucuucca ucguccccac ccacagcaca aaauauggcc ucccgcugaa gaccaaacuu     3360

cugaugggac aacucgugcu gauguucgag gaaaauccug augagauuca gguugacaac     3420

accaaggauc uggugaagcg ccuuuacaaa gucgugggca ucgaaaagga cgggagaauu     3480

aaauuuaagu aucaucagga ggcgcggaaa gaaggacugc ccaucuucuc uacacccuac     3540

aagaauaacg augacuaugc uccuauuuuu agacagagca ucaauaacau caauauucuc     3600

guggauggca ucgacuucac gauugauauc cuggggaaag ucacccucaa ggaguga        3657


<210>  4049
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4049
augaacaaga gaauccuggg ccucgacacc gggacaaaua gccugggaug ggccgugguc       60

gauugggacg agcacgcuca guccuacgag uucauuaaau auggcgaugu uaucuuucaa      120

gaggggguga agauugaaaa aggaaucgag ucuagcaagg ccgcggaacg guccggcuac      180

aaagcuauuc gcaagcagua uuucagacgg cgccuuagaa agauccaggu gcugaaaguc      240

cucgugaagu accaucugug ccccuaucuu ucugacgaug accugcggca guggcaccuc      300

caaaaacagu acccuaagag cgaugagcug augcuguggc agcgcacguc cgacaaaaag      360

gggaagaacc cguauuacga uagacaucgg ugucuccacg agaaacugga ccuuaccguu      420

gaagccgauc gcuacacacu gggcagagcc cucuaucauc ugacccagcg gcgcggauuu      480

cuuucuaaua gacuggacac aagcgcugau aacaaggagg acggcguggu caaauccggg      540

aucagccaac ucucuaccga aauggaggag gccggaugcg aauaccuggg cgacuauuuc      600

uacaagcugu augaugagca ggggaauaaa gugcggauuc gccagagaua cacagaccgg      660

aacaagcacu aucagcacga auuugaugcu aucugugaga aacaagaacu cuccagcgag      720

cugauugagg accuucagag agccaucuuc uuucagcugc cccucaaguc ucaacgccau      780

ggaguuggcc ggugcaccuu cgaaagaggg aagccucgcu gugcggauuc ccaccccgac      840

uacgaggaau uucggaugcu gugcuucgug aauaacauuc aggucaaagg accucaugau      900

cuugagcuga gaccccucac guacgaagaa cgcgagaaga ucgaaccucu guuuuuccgg      960

aaaagcaagc cgaauuuuga cuucgaggau auugccaaag cucuggccgg caagaagaac     1020

uaugcuugga uccacgacaa agaagagaga gccuacaagu ucaauuaucg caugacccag     1080

ggcgugcccg gguguccuac aaccgcgcag cucaaaucua ucuuuggaga ugacuggaag     1140

acaggcauug ccgaaaccua cacgcugauc cagaaaaaga acggguccaa gagccuucaa     1200

gagauggagg acgaugugug gaauguccug uauuccuucu cuagcguuga aaaacucaag     1260

gaguuugcuc accauaaacu gcagcuugac gaagaguccg ccgagaaguu cgcuaaaauu     1320

aagcugagcc acucuuuugc cgcccucucc cugaaggcua uccggaaauu ccugcccuuu     1380

cucagaaagg gaauguacua uacacaugcg agcuucuuug ccaacauucc uaccaucgug     1440

ggcaaagaaa ucuggaauaa cgagcagaac cgcaaguaca uuauggaaaa ugugggggag     1500

cuggucuuca acuaccaacc caaacaccgg gaagugcagg gaacaaucga gaugcuuauu     1560

aaggauuuuc uggcuaauaa cuucgagcuc ccggccggcg ccaccgacaa acuguaucau     1620

ccuucuauga ucgaaaccua cccccacgcu cagagaaaug aguuuggcau ucugcagcuu     1680

gggagcccuc ggacaaacgc cauccgcaau cccauggcga ugagaucccu ccacauccug     1740

cggagaguug ugaaccaacu gcucaaggaa ucuauuaucg augagaauac ggaaguccau     1800

guggaguaug cucgcgagcu gaacgacgcc aauaagcgga gagccauugc uggauggcag     1860

aaagaacagg auaagcagca caaaaaguac guugacgaga uccgcaaacu uuauaaggaa     1920

gagaccggca aggauauuga acccacacaa accgacgugc ugaaauucca gcucugggag     1980

gagcaggauc aucacugccu guacacaggg gaacaaaucg gaauuaccga cuucaucggc     2040

agcaacccua aguuugauau cgagcacacg auuccgcagu ccgucggggg cgacagcaca     2100

cagaugaauc ugacccuuug ugacaacaga uucaaucggg aaaucaaaaa ggccaaacuc     2160

cccgcucagc ugucuaacca ugaggagauu cuggcccgca ucgaaccuug gaagaacaag     2220

uaugagcaga aaauuaagga aagagauaaa caacggaccu uugcgggaau ggacaaggcc     2280

gugaaagauc uccgcaucca gaagagacac aagcugcaga uggacaucga uuacuggcgg     2340

ggcaaauacg agcgcuucac aaugaccgaa guccccgagg gguuuuccag acggcaagga     2400

acaggcauug ggcuuaucag ccgcuaugcu ggacuguacc ucaagucucu guuccaucag     2460

gccgacucca gaaauaaaag caacguguau guugugaagg gcgucgcuac ggccgaguuu     2520

cggaaaaugu gggggcugca guccgaauac gagaagaaau gcagagauaa ucacucucau     2580

cacuguaugg acgcgauuac caucgccugc auuggcaagc gcgaauauga ucuuauggcu     2640

gaguacuauc ggauggaaga gacauucaga cagggacgcg gcagcaagcc uaaauuuucc     2700

aagcccuggg ccaccuucac agaggacgug cucaacaucu acaaaaaucu gcugguugug     2760

cacgauaccc cgaacaauau gccuaagcau acgaaaaagu acguccaaac cucuaagggg     2820

aaagugcucg cucagggaga cacagcccgg ggcagccugc accuugauac cuauuacggg     2880

gccaucgaaa gagacggaga gauucgcuau gugguucgga gaccccuguc cagcuuuaca     2940

aagccuaaag aacucgagaa caucgucgac gaaaccguga agcgcacgau uaaagaggcu     3000

aucgcggaua agaauuucaa gcaggccauu gcugagccca ucuacaugaa cgaagacaaa     3060

ggcauccuga uuaagaaagu gcggugcuuu gccaagucug ucaaacaacc uaucaauauu     3120

agacagcauc gggaucuguc caagaaggag uauaaacagc aguaccacgu gguuaacgaa     3180

aauaacuaua ugcuugugau cuacgagggg cucgucaaga auaaaguggu ucgcgaguuc     3240

gaaauuguga gcuacaucga ggccgcuaag uauuacaaaa gaucucaaga ccggaacauu     3300

uucuccagca ucguccccac acauucugcc aaguauggcc ugccgcugaa gaccaaacuc     3360

cugaugggac agcuugugcu gauguuugaa gagaacccug augaaaucca gguugacaau     3420

acaaaggauc ucgugaaacg ccuguacaag gucgugggca uuaaaaagga cgggagagug     3480

aaauucaagu uucaccagga ggcgcggaag gauggacugc uuaucuucag cacccccuau     3540

aaaaacaaua acggcuacgu ccccauuuuu agacacucca agaaaaauau caacauucug     3600

guugacggga aggauuucac caucgacauc cucggaaaag ugacacugaa ggaguga        3657


<210>  4050
<211>  3660
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4050
augaaggagg aagugagagg caaaaaccgg agccagaccu gggccauccu ggcuaaguuc       60

uuugucuggu ccgagaaaga aucuguuugg aaugagaagg aagcccgcac agaggagaga      120

gugaaagaau acaagcucgu ggggcugaag cgggacacga aaggaaacga gcuuaagcug      180

gaaaauuauc gcuggagagu ccaagcgcuc agcgagcagc ugaaccccga gauuguggcu      240

aucguucuuc aggaaauuaa uggccagauc uccgccucua gcggguaccu gggagccauu      300

uccgaucggu cuaaacaacu cuauuucaac cgccagaccg ugggccagua ccugaugagc      360

gagcuggaaa aggacccuaa ugucucccuc agaaaccggg uguuuuaucg ccaggauuac      420

cuggacgagu ucgauagaau cugggaaaaa caagcugagu uucacgagga acuuacaguu      480

gagcugaaga aggaaauccg ggacauuauc auuuucuauc agcgcagacu caaaagccag      540

aaggggcuga ucucuuuuug cgaguucgaa aaacgggagg uggucgugga ggaagaugga      600

cgcaagaaaa auaagaccgu gggcuguaga gucauuccgc ggucccaucc ccuuuuucag      660

gaguucaaaa ucuggcaaac acugaacgac auugaaguuu uugccuggga uaagcagagc      720

aagagaaaga aggcugacaa aucuuccauc cucuucgaua auagcgagga cgcccugcug      780

guggaaggca agcgcuuucu cuaccaggag gagaaagaac ugcuugcgaa ggagcuguuc      840

gugaaggaau cuaugaaaaa ggccgagguc cucaaacugc uuuucgagaa cuaccaagaa      900

cuggaucuca auuuuaagca gaucgacggg aaccacaccg gauucgcucu guuuuccgcc      960

uauagcaaaa ugauugagaa guacggcuau gaaccugugg acuucaagcg guccgcugau     1020

gagaucauug aaaaacugga gacaaucuuu accaaucucg gguggaacac ggagcugcuu     1080

ucuauugacc ugagcaagga agauaaagag cucgaaagac aguccuacuu ccgccugugg     1140

caucuucugu auagcuuuga gggagacaac acccccacag gcaaugggaa gcuccuggag     1200

aaaaucaugc agcugugcga uguugagaag gaguacgccg uggaacucgc gaacgucucu     1260

uuccaagagg acuauggauc ccugagcgcc aaggcuauca aaaagauucu uccuuaccug     1320

aaagaaggca aucaguacga uguggccugu gacuaugcug gcuaccggca cucuagaagc     1380

ucccucacca aggaugagau cgaaaacaaa auucugaagg acaaacugga gauccuuccc     1440

aaaaauucuc uccgcaaccc uguuguggag aagauucuga aucagauggu caaccugauc     1500

aauacacuca ucgauaccua ugggaaaccg gacgaaauuc ggguggagcu ggccagagaa     1560

cuuaagaaaa acgccaagga gcgcgaggaa cugacgaaaa gcaucgcucg gaauacaaga     1620

gagcaugacg aaauucgcca gcuccugcgg accgaguuug gaaugaugaa cgucuccaga     1680

aaugauauca uucgguacaa gcuguaugaa gaacuuaagg acaacggcua caaaacacuc     1740

uauagcaaug aguacauccc ccgcgaaaag auuuucucua aagagaucga uaucgaacac     1800

gugauuccuc aagcgagacu guuugacgau ucccugagca acaagacccu cgaguaccgg     1860

gccaucaaca uugaaaaagg gaauaagacc gcuuaugacu ucguuaagga gaaauacgga     1920

aacgauggcc uggagaaguu ucuuaauaga ugcgaaacac uguucaaaga caagcgcacg     1980

aaacuccgga agcugaagau ggaggaaaaa gauauccccg agggguucau ugacagagau     2040

cugcgcaaca cccaguauau cucuaagaaa gccuuugcca ugcuuaauga aaucuccaga     2100

cggguggucg cuacaaccgg aagcauuaca gacaagcucc gcgaggauug gcagcuggug     2160

gaccugauga aagagcucaa ccugccuaag uacgaaaaac uuggcauggu ugagauguuc     2220

gaagacaagg auggcagaaa gaucaaaaag auuaccgacu ggacgaaacg gaaugaucac     2280

cgccaucacg ccguggacgc gcugacaguc gcuuuuacca aggauguguu cauccaguuu     2340

uauaacaaua aaaacgccgc cuggaugccc gcuuccaagg agcaugccaa uauuaccggg     2400

aucaaggcua gauacuucga gaacggaaaa gcccucgcgc cgaugccucu ggcccaauuu     2460

cgggcugaag ccaagcgcca ccuggagaau cuucucgugu cuaucaaagc uaagaacaaa     2520

guuauuacaa gcaaugucaa cagaaccaag aacaagaaug gcgaaaaccg gaaacugcag     2580

cagacacccc gcgggcagcu gcaccucgag acgguguaug gaucccauag acaauacgcc     2640

accaagaucg aaaaagugaa ugcggccuuc gacgucacaa agauugcuac cgugucuaaa     2700

cuggccuacc ggaacgcccu ucugaagaga cucgaggcuu uuggcaauga uccuaagaaa     2760

gccuucacag ggaagaacac ccuggagaaa aauccccugu aucuugacga aaaccagacg     2820

gagaugguuc ccgaaaaggu gcagaccguc gaguuugaaa caguguacac cauccgcaaa     2880

ccuauugauc cgaaucugag caucgacaag guuguggaug ucaagauccg guccgugcuc     2940

gagaaaagac ugaaggagua uggcggagac gcuaaaaagg cguucguuaa ccucgaugaa     3000

aaucccauuu ggcugaacga ggccaaaggc aucagcauua agcgcguguc uauccggggg     3060

auuuccaaug cucaaagccu ucacgagaaa aaggacaagg acggaaaccu gauccucggc     3120

aaagaaggga gaaagauucc ugucgauuuu gugaauacag gaaacaacca ucacguggcc     3180

aucuacaaaa agcccguccu ggacaaaguu ggccaggugg ugcuggauga agaggggaau     3240

ccuaaguaug agcuugacga agucgugguu ucuuucuucg augccgugac ccgcgcuaac     3300

cugggccagc ccaucauuga caaggauuac cggagauccg agggauggca guuucucuuc     3360

agcaugaaac aaaaugaaua uuuugucuuc ccgaacgaca agacgggcuu uaauccuaaa     3420

gagguggauc ugcucaaccc cgagaauuac gcccugaucu cuccuaaccu uuuccgcguu     3480

cagaaguuug cguacaaaaa uuauguguuc cggcaucacc uggaaacaac cauuaaggac     3540

acaagcucca cccucaaggg gaucaccugg acagauuuua gaagcucuaa aggacuggac     3600

aagauuguca aagugcgggu ugaccacauc ggccagaucg uguccauugg ggaguacuga     3660


<210>  4051
<211>  3663
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4051
augaagagaa uccugggccu cgaccugggg agcgccucca uuggaugggc ucuuaucgag       60

gaagaugaga aaggcauucg gaucauugcc cuggggucuc gcaucauccc cuacaacgga      120

accgaaggcc aggacuucgc gaagggggcu ggagagagca gaaauucccu ccggacacaa      180

gcccgcacga ccagaaaagg cuaugaucgg uaccagcugc gcagaaagua ucuuauuaac      240

gugcuggaga aaaaucucau gaugccuucu gaagaccugc ggaagaugcc gaagaugaac      300

cuguggcagc uccgcagcaa agccgucaca gaguacaucu ccagacagga acugggccgg      360

cuucugcucu ggcugaauca aaagcgcggg uauaaaucua gcagauccga ugcuaaccuu      420

gacaagaaag auaccgacua cguuaaggag gucaagagcc gguuugauaa aauuaaggag      480

aaaaaucaga caaucggaca guauuucuac gaaucucugc agugcgacga guacuuucgc      540

gugaaggaaa acguguuccc cagagaggcc uauauugcug aauuugaugc caucuguucc      600

cggcaaaaag accaccucgg ccugagcgag gaacugauug cggagaucag aaaugagauc      660

auuuacuauc agcgcccucu caagucucag aaagggcugg uguccguuua cgauuucgaa      720

ggaaagugga ugaaccagaa uggcaaggag cuuuuuaucg ggcccaaagu ggcccauaag      780

agcucuccuc uguuccaacu cgcuaaaauu ugggaauccg ucaacaauau caagcugagc      840

accaaauaug gagacacaau ugagcuuacc cuggaagaga agaagcagcu cguggaguuu      900

cuggacaaca augaaaaacu gacgaccgcc gcucucuuca agauccugcg gaaagauaag      960

aaagaguaca uccuucccaa gcagcuggaa aagggccucc aaggcaacau uacaaaaugc     1020

aagaucgccg acauucuggg guccaaauau aaccagcuuc ugcagcucaa ucuggagaag     1080

caggaaguug gaaccccuug uuaccuguac acaaaaucua ccggcgaggu gcucggggag     1140

aagccgcugc aauauaucga uaagagcguc gaaaaagagc uguuuuacca gcuuuggcac     1200

acgauuuauu ccaucagcga cgaagaugag ugcuguaagg cgcuccagaa aagauucgac     1260

aucgaugccg agacagcugg acgccuggcc aagauugacu uuucuaaaua cggcuucggg     1320

aacaaguccg cuaaagccau ccggaagauu cugcccuauc uuauggaagg agauaaguac     1380

agcgacgcca ugugcuaugc uggcuacaau cauucuaaca gccucaccaa agaggaaaau     1440

cacgagagaa agcugaacga uaaacugaag cuccugacca aaaauucccu ucgccagccu     1500

aucguggaaa agauuaugaa ccaaaugauc cagcugguga auacaauuau cgacaaguac     1560

ggcaaacccg acgagauccg gcucgagcug gcgagagaac ugaagcaguc uaaagaggaa     1620

cgcaacgaug ccgacaaggc uaugagcaaa cggcagagag agaauaagga agccgagaag     1680

aaacuugagg aacaugggau ucgcgccacc cacaacaaua uccucaagua ucggcuguuc     1740

gaggaaaugg gagagaaaaa gcacgaagcu auuuguaucu acugcggcaa cccuauuucc     1800

uuuagcgccg cgaugaaugg ggaugagguc gacguugagc auaucauccc cagaucuaaa     1860

cuguucgaug acucccaaag caacaagaca cucgcucacc ggaaguguaa cgccaauaaa     1920

aacgauauga cggccuauga cuuuaugaag accaaaucua agcaggauuu cgacaaauac     1980

guggaacgcg ugaaucagcu guaugcugau agaauuaucu cccggagcaa guacaacaaa     2040

cuucugaugu ccgaggacga uauuccgucu aauuuuaucg acagacagcu ccgcgaaagc     2100

caauauauug ccacaaaggc ucgggagauc cugcagacca ucugcuacaa cgucuggucc     2160

acaucuggaa ccgugacggc cgagcugaga caucuuuggg gcugggacga uauuacaaug     2220

aaucuccaga ugccuaagua caaagaacug gggcugaccg agaugguuga auggaccagc     2280

gaccacggca agaacgugca cuccaaagag gucaucaagg gauggacaaa acgcgaugac     2340

cauagacacc augcgauuga ugcccucacc aucgcuugua caaagcaagg cuucauucag     2400

cgguuuaaua cgcugucuag cuccaagacc cgcgacgaua ugcagaaaga acuugagaag     2460

ugcagcaucc aguucagaga gaaacggucu cugcucgaaa aguauaucau uucccaacgc     2520

cccaucagcg ugucugacgu ugccgaggcu gugagccgga uucugaucuc cuuuaaagcc     2580

gggaagaagg ucgcgacaau uggaaaaaga aaggugaaag gcaaggucgu gcagcagggg     2640

aucguugugc cucgcggagc ccugagcgaa ggcucugucu acgggaaaau uagaaccauc     2700

gugcggaaga ugcccguuaa guaucuuuuc gagaaccccg aucucaucuu uaaaccuuac     2760

auuaaggcuc uggugaaaga acgccuggcc gaguucgaca augauaacaa gcaggcucuc     2820

gccucccuga aaaagaaucc ggucuaucuu gacaaggaga aaacaaagcu gcucgaauac     2880

ggaaccugcu uuaaagauga guaugugaag aaauacgacg ugaaccccaa uuucacgaag     2940

aucgccgacg uuauugaugg caaagucaag caaauccugg aagacagacu gagcaaguuc     3000

ggcgggaaac cuaaggaggc uuuuaaagau guggugaagg acggaaaaac ccuuaagugg     3060

uacgaagaug agggccugga gcggcccauu agauccgucc gcugucucac agggcugucu     3120

gcggugguuc cugugaagaa agacgaaaac ggaauggaua ucggcuucgu caagcccggg     3180

aacaaucacc auguggccau cuauguuggc gcugacggaa aaggccagga gcacgugugc     3240

accuuuugga acgccgucga acggaagaaa uacgggcucc cgauuaucau uaaggaugcc     3300

aaugaggugu gggacaagau cacaauggcu ggagaugacg uuuauccuca gagcuuccug     3360

ucccuucugc ccaaaccuga cuggacccuc aagcugucua ugcagcaaaa cgagauguuu     3420

auucugggca uggaagagga ugaagugaaa uucgccaucg agagcaagga cuacaaacuu     3480

cuguccgauc accucuauag aguccagaag cugucuagcu ccgacuacaa uuuuauccau     3540

caccucgaaa cggaugugca gaagaaaaca augaccuauc gcgugcggag ccugucuggg     3600

cuuuucgcgc ugagacccuu uaagguccgc auugacuguc ucggaaacau cauuccuucc     3660

uga                                                                   3663


<210>  4052
<211>  3669
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4052
augaagaaaa gaauccuggg ccucgacagc gggaccaacu cccugggaug ggccgugguc       60

gaucgggacg agaauaacca guacucucuu auuaaguaug gcgaucugau cuucgaagag      120

gggguuaaaa uugaaaaggg aguggagagc uccaaagcug ccgaacgcac aucucacaga      180

gcgcuccgga agcuguacuu ucgcagacgg cuucgcaaga ucgaggugcu gaaaguccuc      240

gugaaguaua aucugugccc ccaaguuagc gaggaggaac ugcaucucug gcacacgaga      300

aaacuguacc cucagaacaa ggaccuucug caguggcagc ggaccgauga aaaagcuggc      360

aagaauccgu auauuuaccg cuauagaugu cuccaugagg agcuggacau guccgugucu      420

gccaaccgga ugacagucgg gcgcgccaug uaccaccuug cucaaagacg gggauuccug      480

agcaaucgcc ucgauaacga agaggacgaa aaucagaucg gcgccgugaa gugcggcauc      540

uccaaccuga ccgaggaaau ucagagcucu ggguguaaau accugggaga guauuuuuac      600

aagcucuaua augagcaugg caacacaguu agacugcgga ccagauacac aucccgcgag      660

uuccacuaua aaaaggaauu uuacgcuauc ugcgaaaaac agaagcugga uaaaaagcug      720

auugaggaac ucgccagcgc gcuguauuuc caacggcccc uuaagucuca gagacacacc      780

guggggcgcu guacguuuga gccuaauaaa ccccggugcg ccgacuccca uccugcuuac      840

gaggaguuca gaaugcugag cuuuaucaac accauuaagg uccagggacc cuacgauaca      900

gaacuccgcg ugcugaauac cgaggaacgg gacucuauca caaaacuguu cuguagaaag      960

uccaaaccua acuuugauuu cgaagacauu gccaaggagc ucgcuggccg caacaauuau     1020

ugcuacaucg gggacguggu cgauaaggcc uauaaauuua acuaccggau gagccaggga     1080

guuuccggcu guccgaccac gacauaucug aucaaugugu ucggccaaga cuggcagacc     1140

gggauugcgg agucuuacca ggccaucgau acaaaacuug gacaaaagag cguggacgcu     1200

augauugaug acgucuggaa cgugcuguau uccuucagcu cuaaggauaa acucagagac     1260

uuuggccgca agaagcugca gcuuggggau aaagaagccg acaccuucgc uaauaucaag     1320

cugacccaca aauuugccuc ccucagccug aaggccauuc ggaaaaucaa caucuuccug     1380

agaaugggac ucguuuacuc ucaugcugug acacuggcga auauucccac gaucgucggc     1440

accgauauuu ggcagcggaa gcgcaacuuu auccuugccg agcugcuccc ucugauugaa     1500

aauuucaacc ccaaggagac aaaacugcag gagacccuug acuuuugcgu gaaggacuuc     1560

cuccaaaaua acuuugaacu gaaaaauggg gcuguugaua agcuguacca cccuagcaug     1620

aucgaggccu aucccgacgc cggacucaac uccaauggca uccugcagcu uggcucuccg     1680

agaacaaacg cuauucggaa cccuauggcc augagaagcc ugcacgaaau ccgcaaagug     1740

auuaaucagc uccugaagga uaaaaucauu aaccaggaca ccgagaucca ugucgaauac     1800

gcgcgggagc ugaaugaugc uaacaagaga aaggccauuu ccaucuggca aaagagccag     1860

gagaaacuuc acaaaaagua uguggaagag aucacgaauc ucuacaaaga agagacaggg     1920

aaggccauug uccccaccga caccgauauc cugaaguuca aacuguggga ggaacagaac     1980

cauauuuguc ucuauacagg acagcaaauc ggcauugcug aguuucuggg ggccaccccu     2040

aaguacgaca ucgaacacac aaucccccag ucugugggag gcgauuccac ggacaugaau     2100

cuuacccugu gcgauagccg cuucaacaga gagauuaaaa aggcucagcu ccccacacag     2160

cuggggaauc augccgacau ccugguucgg auugcgggcu ggaaaaacuc uuaugaaaaa     2220

cuuuccaagg auaucgaccg cauuagaacc cacagcggaa uggccaagga caucaaggau     2280

cggcucaucc aaaaacgcca ucugcugaag cucgagagag acuacuggcg gggcaaauac     2340

gaacgcuuua caaugaauga gauuccugag ggguucucca gacggcaggg agugggcauc     2400

gggcugauuu cuaaguaugc uggacuuuac cuuaagagcc ucuuccacga uaaaaacgaa     2460

cgcaauaagg gccacgucuu ugugguuaaa gggaccacga ccgccgaguu cagaaagaug     2520

uggggccugc agaacgaaua ugagaaaaag ucccggaaua accaugugca ccacugcauc     2580

gacgcuauua caaucgccug uaucggaaaa caugaguacg cgcaaauggc ccaguauuac     2640

cacgaugaag cugaguaucu guaccaucac ggcauuaagc cgaucuuucc caagccuugg     2700

aaaaccuuca cagaagaccu ucucgaucug gagcauucuc uguuugucgu gcaccacagc     2760

cccaacaaua uggccaagca ggcuagaaaa cgcauuauca ccuccacggg gcagcauugg     2820

gcccaaggag acagcgccca cggcucucuc cauaacgaua cauacuaugg ggcuauugaa     2880

cgggacggag agaucaagua cgugguuaga cgcgcggucu ccagcuucga gcagaugucu     2940

gauguggacu ccauugugga ugacgucgug aaagaaaccg uucggaaggc ccuggagggc     3000

aagaauuuua aagaagcugu gaacgccccu aucuauauga auaaggagaa agggguccuu     3060

aucaagaaag ugagaugcua cgccaagagc auuaagaaac ccaucgacau ucgcacacac     3120

cgggauucua gcuccaagga auauaaaaga aaguuccaug uuagcaacga caucaauuac     3180

cugauugcua ucuaugaggg caccgugaaa ggaaagguca agcgcgauuu ugaggugguu     3240

aaaaacaucg aagccgcgaa uuucuuuaag cggucucagg acagacggga uuacccgacc     3300

auugugccug agaaauccau caagcacggc uucccccuca aaacaacgcu gaccaacggg     3360

acaaugguca uucuguacga agagaauaag gaagagaucg acuuuaccaa gaaagaggau     3420

auuugucgca gacuuuauaa gcugguggga augagcucca ugaucguguc uggcugcgaa     3480

uacggggucc ucagccugcg gcaccaucag gaggcucgcc cuuccaaaga caucaagucu     3540

aaaaacggag ccuucaagaa uggcgaagcc cucagacccg cuauuacaau gcugcacacc     3600

caauucaacg cccuuguuga gaauaucgau uuuaacauug acgugacggg gaacaucgag     3660

cugauuuga                                                             3669


<210>  4053
<211>  3669
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4053
augaagaaaa gaauccuggg ccucgacagc gggaccaacu cccugggaug ggccgugguc       60

gaucgggacg agaauaacca guacucucuu auuaaguaug gcgaucugau cuucgaagag      120

gggguuaaaa uugaaaaggg aguggagagc uccaaagcug ccgaacgcac aucucacaga      180

gcgcuccgga agcuguacuu ucgcagacgg cuucgcaaga ucgaggugcu gaaaguccuc      240

gugaaguaua aucugugccc ccaaguuagc gaggaggaac ugcaucucug gcacacgaga      300

aaacuguacc cucagaacaa ggaccuucug caguggcagc ggaccgauga aaaagagggc      360

aagaauccgu auauuuaccg cuauagaugu cuccaugagg aacuggacau guccgugucu      420

gcuaaccgga ugacagucgg gcgcgccaug uaccaccuug cccaaagacg gggauuccug      480

agcaaucgcc ucgauaacga ggaagacgag aaucagaucg gcgcugugaa gugcggcauc      540

uccaaccuga ccgaagagau ucagagcucu ggguguaaau accugggaga guauuuuuac      600

aagcucuaua augaacaugg caacacaguu agacugcgga ccagauacac aucccgcgag      660

uuccacuaua aaaaggaauu uuacgccauc ugcgagaaac agaagcugga uaaaaagcug      720

auugaggaac ucgcuagcgc ccuguauuuc caacggcccc uuaagucuca gagacacacc      780

guggggcgcu guacguuuga gccuaauaaa ccccggugcg cggacuccca uccugccuac      840

gaagaguuca gaaugcugag cuuuaucaac accauuaagg uccagggacc cuacgauaca      900

gaacuccgcg ugcugaauac cgaggaacgg gacucuauca caaaacuguu cuguagaaag      960

uccaaaccua acuuugauuu cgaggacauu gcuaaggagc ucgccggccg caacaauuau     1020

ugcuacaucg gggacguggu cgauaaggcu uauaaauuua acuaccggau gagccaggga     1080

guuuccggcu guccgaccac gacauaucug aucaaugugu ucggccaaga cuggcagacc     1140

gggauugccg aaucuuacca ggcgaucgau acaaaacuug gacaaaagag cguggacgcc     1200

augauugaug acgucuggaa cgugcuguau uccuucagcu cuaaggauaa acucagagac     1260

uuuggccgca agaagcugca gcuuggggau aaagaggcug acaccuucgc caauaucaag     1320

cugacccaca aauuugcuuc ccucagccug aaggccauuc ggaaaaucaa caucuuccug     1380

agaaugggac ucguuuacuc ucaugccgug acacuggcua auauucccac gaucgucggc     1440

accgauauuu ggcagcggaa gcgcaacuuu auccuugcgg aacugcuccc ucugauugag     1500

aauuucaacc ccaaggagac aaaacugcag gaaacccuug acuuuugcgu gaaggacuuc     1560

cuccaaaaua acuuugagcu gaaaaauggg gccguugaua agcuguacca cccuagcaug     1620

aucgaggcuu aucccgacgc cggacucaac uccaauggca uccugcagcu uggcucuccg     1680

agaacaaacg ccauucggaa cccuauggcu augagaagcc ugcacgaaau ccgcaaagug     1740

auuaaucagc uccugaagga uaaaaucauu aaccaggaca ccgaaaucca ugucgaguac     1800

gcccgggagc ugaaugaugc gaacaagaga aaggcuauuu ccaucuggca aaagagccag     1860

gaaaaacuuc acaaaaagua uguggaggaa aucacgaauc ucuacaaaga ggaaacaggg     1920

aaggccauug uccccaccga caccgauauc cugaaguuca aacuguggga ggagcagaac     1980

cauauuuguc ucuauacagg acagcaaauc ggcauugccg aauuucuggg ggcuaccccu     2040

aaguacgaca ucgagcacac aaucccccag ucugugggag gcgauuccac ggacaugaau     2100

cuuacccugu gcgauagccg cuucaacaga gaaauuaaaa aggcccagcu ccccacacag     2160

cuggggaauc augcugacau ccugguucgg auugccggcu ggaaaaacuc uuaugagaaa     2220

cuuuccaagg auaucgaccg cauuagaacc cacagcggaa uggcgaagga caucaaggau     2280

cggcucaucc aaaaacgcca ucugcugaag cucgaaagag acuacuggcg gggcaaauac     2340

gagcgcuuua caaugaauga gauuccugaa ggguucucca gacggcaggg agugggcauc     2400

gggcugauuu cuaaguaugc cggacuuuac cuuaagagcc ucuuccacga uaaaaacgag     2460

cgcaauaagg gccacgucuu ugugguuaaa gggaccacga ccgcugaguu cagaaagaug     2520

uggggccugc agaacgaaua ugagaaaaag ucccggaaua accaugugca ccacugcauc     2580

gacgccauua caaucgcuug uaucggaaaa caugaauacg cccaaauggc gcaguauuac     2640

cacgaugagg ccgaauaucu guaccaucac ggcauuaagc cgaucuuucc caagccuugg     2700

aaaaccuuca cagaggaccu ucucgaucug gaacauucuc uguuugucgu gcaccacagc     2760

cccaacaaua uggcuaagca ggccagaaaa cgcauuauca ccuccacggg gcagcauugg     2820

gcucaaggag acagcgccca cggcucucuc cauaacgaua cauacuaugg ggccauugag     2880

cgggacggag agaucaagua cgugguuaga cgcgcugucu ccagcuucga acagaugucu     2940

gauguggacu ccauugugga ugacgucgug aaagagaccg uucggaaggc gcuggaaggc     3000

aagaauuuua aagaggccgu gaacgcuccu aucuauauga auaaggaaaa agggguccuu     3060

aucaagaaag ugagaugcua cgccaagagc auuaagaaac ccaucgacau ucgcacacac     3120

cgggauucua gcuccaagga guauaaaaga aaguuccaug uuagcaacga caucaauuac     3180

cugauugcca ucuaugaggg caccgugaaa ggaaagguca agcgcgauuu ugaagugguu     3240

aaaaacaucg aggcugccaa uuucuuuaag cggucucagg acagacggga uuacccgacc     3300

auugugccug aaaaauccau caagcacggc uucccccuca aaacaacgcu gaccaacggg     3360

acaaugguca uucuguacga ggaaaauaag gaggagaucg acuuuaccaa gaaagaagau     3420

auuugucgca gacuuuauaa gcugguggga augagcucca ugaucguguc uggcugcgag     3480

uacggggucc ucagccugcg gcaccaucag gaagcgcgcc cuuccaaaga caucaagucu     3540

aaaaacggag cuuucaagaa uggcgaggcc cucagacccg ccauuacaau gcugcacacc     3600

caauucaacg cucuuguuga gaauaucgau uuuaacauug acgugacggg gaacaucgaa     3660

cugauuuga                                                             3669


<210>  4054
<211>  3678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4054
augagcaaga gaauccuggg cauugacgug gggaccaacu cccucggaug ggccgucguu       60

gaucggacag acugcgauaa uuacacgcug gugaaacgcg gcgacgugau cuuccaggag      120

ggggucaaca uugaaaaggg aaauaucgag aaaucuagag cuagcgaacg gaccggccac      180

cgcuccacaa gaaagcauua uuuucggcgc agaaugcuua aaauuaaggu gcuggagauc      240

cucguugaac accaacugug ucccuaccuu ucucaugagg agcugcacca guggaugacc      300

cggaaggugu auccuacaaa cgaagauuuc cgccucuggc ugagaaccaa ugacgccaac      360

gaucagaauc cguaccauga ccggcacguc ugccugcacg agcgccucga ucugagacuu      420

caggaaaacc gguaucgccu ggggagagcg cucuaccauc uggcucaacg gagaggauuu      480

cuuagcaauc gccuggagca gaacuccaaa acagacgaga ccggcgccgu gaagucugau      540

aucagccagc ucaaugaaga gaugaaaaag gccggcuccc aguaucuggg ggacuacuuc      600

uacuggcugu aucaagaaua cggaaaccag guuacgcucc ggauuagaua uaccgaucgc      660

aaacagcacu accagaagga guuugaagcu aucugucaac ggcagcagcu gagcgacgac      720

cuuguggaua agcugagacg cgccauuuau uuccaacggu cucucaaauc ccagagagcu      780

ggcgucggga agugcacauu ugagcccgac aaaccucgcu gugcccugag ccauccccgg      840

uacgaggagu ucagaaugcu uucuuuucug aauaacguga aggugaaagg accuuccgau      900

gacagccucc gcccccuguc uccuuccgag aaggaaaaga ucaagccgcu guuccucaga      960

aagagcaaag cgaauuuuga uuucgaggac auugccaagg cuaucgccgg caaagggaag     1020

uaugcuugca uucgggauaa agaaggagcc cagaagcccu accaguuuaa cuacgcgcug     1080

ucccaaggcg ucucuggcug uccuguuacc gccaaacuua ucgcuguguu cggggagagc     1140

ugggagcgga accuggugaa ugccuaucgc uccagaaacg acaugcggaa aagcgaugaa     1200

gagaucguca augaagugug gaacguucuc uacucuuucu ccagcaagga gaaacugaga     1260

gcuuggggaa uggaaaaacu uggccuggac gaggccugcg ccggggaguu ugcugauauu     1320

acacucccca ccgcguucgc cgcucugucu cugaaggccg ugaauaagau ccucccuuuu     1380

cugcgccagg gauucauuua uagccacgcc gucaugcuug cuaacauccc cuacauuauc     1440

ggcauggacc gguggaauga accgagacag cgccaguuua ucauggacaa ccugcaagag     1500

cuccuggaua auuucacggc cuccgacaag gcgcugcagc agacacuuga uuuuuguauu     1560

aaagacuucc uccugaacaa uuaugaacug gcuccuggga aggccgauag acucuaccac     1620

cccucuauga ucgagaccua uccugacgcc aaagcugaug gacagggcau uuaccaacug     1680

ggcagccccg ccacaaacgc uauccggaau cccauggcca ugcgcucccu ucauauucug     1740

agacgggugg uuaaccagcu ccugaaggag aaaaccaucg acagcgaaac cgaggugcac     1800

aucgaauacg cgcgcgagcu gaaugaugcc aacagacggu gggcuauuaa ccaguggcag     1860

cgcaagcaag aaacacagag aaaggacuau gccgagaaaa uccggaaggu cuacgcugau     1920

gaguuugaaa aagugauuga gccuacgcag gaagaacuuc ucaaguauca acugugggag     1980

gaacagcagc aucugugccu cuacaccggg gagcagaucg gacugacaga cuucauuggc     2040

ccgaaucccc gcuaugauau cgaacacacc auuccucaau cuaugggagg ggacuccaca     2100

agaaugaacc uuacccugug ugagcggacg uacaauagaa cagucaaagg caaccgcaug     2160

cccagccagc ucgccgaaca ugagucuauc cugacccgga ucaagccuug gaaagacaag     2220

uaugaggaac ugaccgugaa gauuaauaaa cuuagaacac accccgggau ggauaaggcg     2280

uccaaagaca acaucauucg caagcggcac cuccuggagc uggaaagaga uuacugguuu     2340

cagaaauacc agcgcuucac caugacagag guuccggaag gcuuuagccu ccggcaagga     2400

acgggcaucg ggcugauugg aaaguaugcc ggccuuuucc ugaagucccu cuuccaucac     2460

gugaaagaga gaaaucgcaa gcaggucuac gugaucaaag ggccuaucac cgcugaguuu     2520

cggagacugu ggggacugca gaaccuuuau gaacagaagu cucgggacaa ucaugcccac     2580

cacugcauug augcuaucgu uauugccugc aucaaccccg ccgaguacgc ucgcauggcg     2640

agcuauuaca gacaagaaga ggacuuccgg cucaauaaag gcaacaagcc uguguuuccc     2700

aagccuugga caaccuucac acaggaucug caggcccugg aagcugagcu ccuggucgug     2760

caugagaccc gcaauaaccu uacgaaaaag gccagaaaac gggugagaac caaagggggc     2820

aauguucugu ccgaaucuga cagcgcccgc ggaucccucc acaacgauac auauuacggc     2880

gcuauucaaa aggacggggu cauccgguac guggugagaa aggcccugag cucucugucc     2940

gagaaggaug ucaaagacau cguggauaag accguucgcg aaaaagugca ggcggcuauu     3000

caggagaagg gagccaaaga agcccuugcu ggcacaaucu auaugaacga ggagaagggg     3060

auuaccauca aaaagguccg guguuuuacg aagauugcca gcccccuggc gaucagacgc     3120

caucgggacg cuucugccaa agaauacaag agaccguauu acgugacaaa ugaguccaac     3180

uauguuaugg gaaucuacga aggcugcauu aaagagaaga ccaaacgcga guucauccuc     3240

gugaauaacc uggaagccgu cagacagcuc aaggcugccc ggaagcaagg ggugguuagc     3300

ucuauucugc cucccgugag cgcuaaaucc caguuucccc uuaaguaucg ccuguacaca     3360

ggcacccucg uccugcugua cgagaauagc ccugaagaga ucgacuucac caaccaggug     3420

gauauuugua aaagacuuua uaaggugaca ggacugucuu ccaugacggu caccggcaau     3480

caguacggga caauccaacu ccgguaucac caggaagccc gccaggcgaa agaccugaag     3540

gcccagaacg gagcuuacaa ggagggcgag gaacauagac cggccauuau gaugcuccac     3600

acccaauuua aagcucuggu ugaggggaca gauuuccgga ugaauauccu uggagaaauc     3660

gagccccuga gaaaguga                                                   3678


<210>  4055
<211>  3690
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4055
augggcgaca gaaaguacaa ccuggggcuc gauaucggaa ccagcuccau uggcuucgcc       60

gcuguggacg agaauaacca gcccauccgg gucaaaggga agacagccau uggaguucgc      120

cuguuugaag agggcaaaac ggcggcugau agacgggggu uccgcaccac aagacggcgc      180

cuuucuagac ggcgcuggag aaucaaucug cucaacgaaa uuuuugacgc ccaccuggcc      240

gagguggauc cuaccuuccu ugcucggcug aaggaaagca aucgcuccaa ccucgacccg      300

aaaaagucuu uucaaggaag ccugcuguuc cccgagagaa aggauuauca guuuuacgag      360

gaauauccua caaucuacca ucuccggaaa gcccugaugg agaaggaccg caaauucgau      420

aucagagaaa uuuaucuugc ugugcaccau aucauuaagu accggggcaa uuuucugaac      480

ggcaccccca ugagauccuu caaagucgag aauaucgagc ucaacacacu guuugaccag      540

cuuaaucagc uguaugccga aauugugccu gauaacgagc ucgcguucga ccuggcccaa      600

guugcugaug ugaaggacgu ccugucuagc accacgaucu acaagaugga caaaaagaaa      660

cagcucguga agaugaugcu gcuucccgcc uccaauaaag cucugcagag cgaaaacaag      720

aaaauuguua cccaguuugu gaaugccauc cucaacuaca aguucaagcu ggauguccuu      780

cugcaagugg agacagacgc ggauuggucu cucaaacuga acgacgaagg ggccgaugac      840

aagcuggagg aguucaccgg agaucucgac gaaaaucgcc uggagaucau ugaucuucug      900

cagcggcucc acaacugguu uucccugaau gaaaucacaa aagacggcaa cagccuuucu      960

gcugccaugg uggagaagua ugaaaaucau caccaccauc uggggcuccu gaaaaagguc     1020

auugagaacc acccugaugc uaagaaagcc aaggcccuga aagagaccua cacggcuuau     1080

guuggaaaga cagacgacaa aacccagaau caggaugacu ucuacaaggc gguggaaaag     1140

aaccucgaug acuccccgga ugccaaaggc aucaagagac ugauucaacu ugaccaguuu     1200

augcccaaac agcgcacagg gcaaaaugga gcuaucccuc aucagcugca ccagcaggag     1260

cucgaucaaa ucauugaaaa gcagagcaaa uauuaccccu uccuggccga gccuaacccc     1320

aaugugaagc ggagaaagga cgccccguau aaacuggaug aacuuaucgc uuuuaagauu     1380

ccuuacuacg ucggcccccu cgugaccccu gaggagcagg cccagaacaa agaaaauguu     1440

uucgcgugga ugaagcgcaa agcugccggc cccaucaccc ccuggaacuu ugacgagaag     1500

guggaucgga uggaaucugc caauagauuc auucgccgga ugacaacgaa agacaccuau     1560

cuguuugggg aggauguccu gccugcugag uccaugaucu accaaaaguu cgugguucuc     1620

aacgaacuga acaaucuuaa gaucaacgga agacaccuga gccucaaaga caagcaggac     1680

guguauaaug aucuguuuaa acagcagaag acagucucca uuaaagcccu gcaaaacuac     1740

uaugugacca agaagaaagc ugccacagcg ccgaccgucg gcgggcuugc cgaccccaag     1800

aaauuccucu cuagccuguc cacguacauc gauuuuaaga auauguucgg agagcgcgug     1860

aacgacccuc aguuccagga agaucuggag cagauuguug aauggagcac aaucuuugag     1920

gaccggggca uuuucaaagc uaagcuccaa gcccuggggu ggcuuucuga gaagcagauc     1980

cagcaacugg uggcuaaaag auauaaggga uggggccggc ucuccaaaaa gcugcugacc     2040

ggccuuaaaa augccgaagg guacagcauu cucgaugaga uguggcgcuc uaccgaaaac     2100

uuuaugcaga uccagagcag acccgaguuc gcggcccuga uccagcaagc uaaugaaaag     2160

caguuugagg gaaacgaccc ugauaauguc ugggagaaca uugaaaauau ccugggcgac     2220

gccuacacau cuccccagaa caagaaagcu auuagacagg ugguuaaggu gguccaagau     2280

aucgagaaag ccguggggaa uccgccugaa aagauugcca ucgaguucac ccgggaagcu     2340

gcggccaacc cccagcgcac acagucccgg cucagaacgc uggagaaacu uuaugagagc     2400

gcugaagagg ugguugacgc cggacugacc gccgaacucg cugaguuuaa ggagaacaaa     2460

cauguccugu ccgauaagua cuaucuguac uucacacagc uuggccgcga cguguauacc     2520

ggggacacaa ucagccucga uaagcugaau gacuacgaug uggaccacau ucugccucaa     2580

ucuuuuauca aagaugacuc ccucgauaac agaguccuga ccauucgggc cgugaauaac     2640

ggcaagagcg acaauguucc cgcgaaaaug uucggaaaga aaaugggcuc uuuuuggcgc     2700

caucuucugg auaacgggau gaucuccaag agaaaguaua auaaccucau uacggacccu     2760

gauaauauca gcaaauacgc ucagaaggga uucaucaacc ggcagcuggu ggaaaccucc     2820

caagucauua aacugacagc caauauccuu aacggcauuu acgacaagga caccgagauc     2880

auugaagugc ccgccaaaau gaacucucag augcgcaaga uguuugaucu gguuaaggug     2940

agagagguca augacuauca ccaugcuuuc gaugccuacc ucacaaucuu caucgggaau     3000

uaucuguaca aaugcuaucc gaagcuccgg ccuuacuuug uguaugacaa cuucaaaaag     3060

uuuggaaaca aagaagauau uggccacaag cgcuucaauu uucuggggaa gaucgagaga     3120

gagaaaaagg uuguggcgcc cgaaaccggc gagauucuuu ggagcaacgu cgcucccaau     3180

gaaacgauca aacagauuaa gaaaguguac aacuacaagu ucaugaucgu gucccgggag     3240

auuacaaccc gcagagccga acuguuuaau cagacagucu auccuaagaa cuaccgggga     3300

aaacucaucc cgaucaagga ggacagaccc accgaucugu auggcgggua cucuggaaau     3360

accgacgccu aucuggcuau uguugcccuu gaggauaaaa agaaaggcaa guacuucaaa     3420

guggugggga ucccuacacg cgcugccgcg aagcuggaaa agcucaaaca acaggacagc     3480

cagcaguauc ugcaagcccu ccacgagguc auugcucccc aguuuacgaa guccaccaaa     3540

aagggaauca aaaagacaga guucgaaauu gugcuggaua agguucauua ccggcagcuu     3600

gugcaggacg gcccugucaa aaugaugcug gggagcucua ccuacaagua uaacgccaaa     3660

caacucgugc uguccgaaaa ggcucuguga                                      3690


<210>  4056
<211>  3696
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4056
augaucaccc ggauucuggg ccucgacaca gggacgaaca gccuggccug ggcugugguc       60

gauagagacg ccaauggaaa cuacacccuu guuaagcgcg gcgaugugau cuucacagag      120

ggggugaaaa uugaaaaggg aaucgagucc ucuaaagcgg cugaaagaac cgcccacaag      180

gcccugcggg uccauuauag ccgcagacgg cuccacaaaa uugacacacu gaaggugcuu      240

guugagcugg aucucugccc cuaccugucc aaugaacagc ugaaccucug gaaggcuaaa      300

aagaccuauc cucugacaga cgauuuuaug cuuuggcaac gcucuggcag cgccgaggag      360

aauaacccgu acuauuguag acaucggugc cugacccaga aacucgaccu gacggaucag      420

ucccagcgcu uccuucuggg gagagcuuuu uaccaccucg cccaacggcg cggauuccug      480

ucuaauagac uggacaccag cgcggauucc gacgaaagcg gcaaggugaa aucuggcauc      540

gccgagcucu ccgucgaaau ggagaaggcu gggugugagu uucugggaga auauuucuac      600

cagcuuuaca agcagcaugg caacacaauc aaacugcgga gccgcuauac cgauagagag      660

gaacacuaca agaaagaguu ucucgccauu ugcgaaaagc aggagcugga ccccaaaugg      720

guggaggcuc uccaacgggc caucuauuuc cagagaccuc ugaagucuca gcgcaaaggg      780

guuggaaagu guacauuuga acccaagaaa ccucggugcg cggaguccca ccccgacuac      840

gaagccuuca gaaugcucug uuuucugaau aacgugaaga uucagacccc uaaugauacg      900

agccugcgcc cgcucaacga ggacgaacgg gagaaaaucu ggccccuguu cuuuagaaag      960

ucuaaaccua auuucgauuu cgaggacauu gcuaaggccc uugcuggcaa gaacaauuau     1020

gccugguaca aagaugccgg ggaaaagccc uuuaaauuca acuaucgcau gcaacaggga     1080

gucuccggcu gcccugugac agcucagcug aucgacauuu uuggcagcga uuggcaaacc     1140

gcgaucgccg agacauacac cgcuguggaa aagaaaaacg ggaccaagac aaucgacgag     1200

auggccgaug acgucuggaa uguucucuac uccuucucua gcaaggauaa acugaaggcc     1260

uuugcuacgg aaaaacuuca gcuggacgag gccuccgcga ccaaguucgc uaacgccaaa     1320

cucccccaug gauuugccag ccugucucug aaggcuauuc ggaauauccu cccguuccug     1380

agacagggcu uuauuuaugc ccacgcugug caucuugcca agaucccuga cauugugaca     1440

aaagcggucu ggaacgaugc cgacaagcgc gagaugaucc uguccgggcu cauggaaauc     1500

auugagaauu acaaccccaa agaucgggga guggaaggca ccaucgacuu cuguauuaag     1560

agccaucugc aggauguuau cgaccugaga ccuggggcug ccgaugcucu uuaucacccc     1620

ucuaugauug agacauaccc cgacgccaaa cucaauaaga ugggaguguu ucaacugggc     1680

agcccucgca ccaacgcgau ccggaauccg auggccauga gaucccugca cauccuccgg     1740

ucugucguga accagcugcu ucgcgauggc auuaucgaca gaaauacgga gguucacgug     1800

gaauaugcuc gggagcugaa cgaugccaau aaaagaaagg cuauugccga cuaccagcgc     1860

gaacaggaga agaaacggaa gcaauauaga gaugacauca uugcccucua caaaagcgaa     1920

ugccauaagg agaucgagcc cacagacacc gauauucuga aauuccagcu gugggaagaa     1980

caggaccacc uuugucucua caccgggaca cagaucggaa ucugcgauuu ucugggcgac     2040

aacccuaagu ucgauauuga acauaccguc ccccgcuccg ugggaggaga cagcacacaa     2100

augaaucuga cgcucugcga ugcuaccuuc aacagacagg ucaagggcgc gcagcugccu     2160

ucucagcuuu ccaaccacgc cgacauccug gugcggauug aucacuggaa aagcaagugu     2220

gaggaccucc gcaaacaacu ggauaagauc agaacauaug ggggcaugga aaaagcuaug     2280

aaggaccggc ugauucagaa gcgccauaga cuuaaaaugg aucucgacua cuggcaggga     2340

aaguauucuc gguuuaccau gacagagguu cccgagggcu ucucccgcag acaaggggcc     2400

ggaaucggcc uggugagcaa auacgccggg cuguuucuca agucccuguu ccacaaugaa     2460

cagaacccga auaaaucuaa cgucuaugug guuaagggag ugaccacggc ugaguuucgg     2520

cgcauguggg gccuucaggg ggaauacgag aagaaaagca gacugaauca uugccaccac     2580

uguaucgacg ccauuaccau cgcgugcauu ggcaaggcug aauaugauaa aauggcccag     2640

uacuaccaug acgaggagcu uuuccaaugg cggggaacaa ccacaaagcc ucacuuuaac     2700

aaacccuggc cuaccuucac ggaagauguc cuggccaucg agcagucccu gcuugugaag     2760

caucucacaa aagacaauau gcccaagcag gcuaagaaaa gagugcgcac cgccacaggc     2820

aaguaucugg cgcaggggga uaccgcucgg ggaucucugc acaaagacac cuacuauggc     2880

gccauugaac aagaugggga gaucagauac guucuccgca agccucugaa caaauuugag     2940

aaugaagccg agcuugaaaa gaucgucgac caggcuguga aggagaaaau uaaggaagcc     3000

aucgcuggga aaaacuucaa gacagccauu acggagccca ucuuuaugaa uaaagagaag     3060

ggcauucgga ucaacaaggu gagauauuac gucaaagcgc aggaacgccu gaagauccgg     3120

cagcaaagag aucucagcuc caaagaguau aagcaguucu uuugugugga aaaugagagc     3180

aacuaccugc uggccauuua cgaaggggag cuuaaaggca agaaggagcg cgacuucugc     3240

guugugaaua accuggaauc ugcugccugu cucaaacggu ccaacaaggg aaaagcugcc     3300

ccgcuggucc cugagagaag cgaggagcgg aaucuccccc ugcuugccgu gcugaagcgc     3360

ggcacccagg uucucuucua ugaaaacucu cccgaggaga uccuguuuga uaauccucaa     3420

gaccugguga gacggcuuua caaagucaca gggauuaaca ccaagaagga cgugaaaaau     3480

ggaaagacau aucuguacgg caacaucgaa cuccgcuucc aucagaaugc ucagaaaaag     3540

gaugacguua aaaugcaguc cgggguguau aagaacaaug agguccugag acaaggaauu     3600

gugcucagcc acaaacaguu uaaggcgcug guggaaggcg ucgauuucga guuuaacguu     3660

cuuggggaga ucaagaaaaa ggaacggcac gccuga                               3696


<210>  4057
<211>  3699
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4057
augaccaaga caagaauccu gggccucgac acggggacca acagccuggg augggccauu       60

guggaucggg acgaggucuc cggccaguac gaacuuguug aucgcgggga ccugaucuuc      120

acagagggag ugaaaauuga aaagggcauc gagucuagca aggcuuccga aagaacccaa      180

caccgggccc uccgcaagca guauuuuaga cggcgccuga gaaaaauuga ggugcuucgg      240

guccugacau ucuaccaucu cugucccccu gugucugagg aagaacugca ccuguggcau      300

guuaagaaga ucuauccggu ggaaaaucac gaguuuauga ucuggcagcg caccaacgau      360

aaugagggga aaaaccccua cuauuacaga cauauuugcc uccacacaaa gcugaaucuu      420

acccugauga aagaccggua uauccucgga cgcgcgcugu accaccuuag ccagagacgg      480

ggcuuccugu ccaaccgccu cgaucaaucu caggacgaag augacaaggg cacggucaaa      540

accgggauua gcucccugag caaggauaug aaugacgcug gaugcacaua ccugggcgau      600

uauuuuuaca agcucuauuc ugagaacggg aauagagugc ggaucagaaa ccauuacacc      660

gcccgcaaug aacacuaucu ggccgaguuc gaagcuauuu gucagguuca gaaacuugag      720

ccugagcugg uggaagcccu ccggagagcu aucuuuuucc aacgcccccu gaagucccag      780

cggaaaggag ucggcaagug cacauuugag ccuagaaaac cccgcugugc cgacagccau      840

ccugacuacg aagaguuccg gaaacuuugc uuuauuaaca auaucaaaau ccagaccucu      900

gaugacgaua cgaugagacc gcugaacaag gaagaacgcc agcggauuaa uucccucuuc      960

uauagaguga gcaaggcgaa cuuuuacuuc gaggaaaucg ccaaacugcu cgggggacgc     1020

aacaauuacu guuauuucaa ggacgaggug caaaaagauu uuaaguucaa cuaccggaug     1080

ucucaguccg ucagcggcug ccccacaacc gcucagcuga gaaauauuuu uggcgaagac     1140

uggaaaacag ggaucgccga gaccuaucaa cacuccgcua agaaggaugg aucucugaaa     1200

accguugacg aaauggccga ugacauuugg aacgugcuuu acagcuucuc cagcaaggau     1260

ugucuguaua auuuugcggu gaacaaacuc cagcuggagg aggacaaggc caaaaaguuc     1320

ucuaagauca aacuuuccca cggcuuugcu agcaucucuc ugaaugccau ucauaagauc     1380

cucccuuucc ugcggauggg gaugauuuac agccacgcug uccuguuugc caacaucccc     1440

acaauugugg gaauggacgu uuggaaugau gaauccaaac gccagcauau caugacggag     1500

cucucugacc ugguguucaa cuaugauccu aagaauagag aagucgaggg caccaucgag     1560

augugcauua aagacuuucu ucugaacaau uacgaucucc ccgccggggc ugugugucgg     1620

cuguaccacc cgagcaugau cgacacauau ccugaugcga aggccaacaa guacggaguu     1680

cugcagcuug gcucccccgc uaccaaugcc auuagaaacc cuauggccau gcgcagccuc     1740

caugaacugc ggcacguggu caaccugcuc cugagaaaag gcaagaucga ccaaaauaca     1800

gaggugcacg ucgaauaugc ucgcgagcuu aacgaugcca auaaaagaaa ggcgauugcu     1860

gaccggcagc gcgaacugga uaaacuccau aagaaauacc acgacgauau cauuacccug     1920

uauaaggagg agacggggaa ggacaucaac cccacacagg ccgacauccu gaaauuccag     1980

cuuugggagg agcaagaaca ugugugccuc uacaccggaa gacagauugg caucucuaag     2040

uuucuggggc ccgauccuga guucgacauu gaacacaccg uuccgcgguc cgugggaggc     2100

gauagcacac agaugaauau gacccugugc gaccgcagau ucaaccggga ugucaaaaag     2160

gccaugcucc ccucucagcu gccuaauuau caagagaucc uuccccgcau ugagaaaugg     2220

aagaagaaau acgaaaagcu gacaaaagac aucgaucacu guaucacgca uuccgggaug     2280

accaaggagg cuaaagacag cauuauccag aagagacacc ggcucacacu ggaacaugau     2340

uacuggcgca acaaguauuc cagauuuacc augacagagg ugccugaagg cuucucucgg     2400

agacagggag ccggcauugg gcugguuagc aaauacgcug gacuuuuucu caagucccug     2460

uuccacgacc ccaccgaucg ccggagaucu cauguguaug ucgugaaagg cacgaccaca     2520

gccgaguuuc gcaagaugug ggggcugcag agcgaguacg agaaaaaguc ccgggacaau     2580

cacgugcacc auugcguuga ugcgaucacc auugccugua ucggaaagaa cgaguauaau     2640

aaaauggcua gcuucuacca cgacgaagag gccuuugaga gaggcgaaug cgagaagccg     2700

cauuucgaca aaccuuggcc cacauuuacc caagaugucc ucgggcugga aaagcgccuu     2760

aucguggugc acucuacgca ggacaacaug ccuaaacaug cuaagaaaua uguccggaca     2820

cccagaggca aguucauugc caagggagau ugugcgcgcg gcucccugca ccucgacacc     2880

uacuacgggg ccaucaaaca caaggaugac acagagccuc gguaugugcu gagacgggaa     2940

cugagcucuu uugauuccgu uaaugaccuu gauauuaucg uggacgaugc uguccgcgag     3000

aaaauuaagg aggccgugaa aggaaagagc ggcaaggcuu ucaaagaagc ccucgccaac     3060

guuuacauga auaaggagaa agacaucaga aucaagaaag ugcggugcua uguccccucu     3120

gugaagcgcc cgauugacau cagacaucag cgggauauga gcagauuuga cuacaagcgc     3180

caauuccacg uugcuaacga ugggaacuau augauggcga uuuacgaagg aguggucaaa     3240

ggcaagcuga aacgggacuu cgagcuggug aagaauaucg aagccgcuuc cuauuuuaaa     3300

gugagccagg auaacgaguc uuucuccagc auugucccug agaaguccuc uaauggguuu     3360

ccccucaagg ccguucugaa aaucggcacc caugugcuuc uguacgaaaa caccgccagc     3420

gagauugacu ucuccgaucc cguggaccuc acaagacgcc uguacaaggu cgugggacug     3480

ucuagcgcua ucaucaauaa cuguuccuau ggcuuuauua cgcuucgguu ccaccaggaa     3540

gccagacagg cgaaaaaucu gaagcucacc aaagggguuu uuaagaacgu ggauaauuac     3600

cgcggagcua ucauucugua ucacacacaa cucaaggccc uggucgaggg cuacgacuuc     3660

gauaucaaca uucuugggga aaucaccucu aaacaguga                            3699


<210>  4058
<211>  3705
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4058
augcccaacu ucuggagcau cuuuaccgag aaucugcaga cauaccucau gaagacgcgc       60

auucugggcc uugacaccgg gacaaacucc cugggauggg ccguggucga ucgggacgaa      120

aauaaccaau auaccccuau cugccgcggc gaucucauuu ucacagaggg gguuaaacag      180

gaaaagggaa ucgaguuuuc ucuggcugcc gaaagaacca gcuaccgggc gucccgcaga      240

cacuauuucc ggcgcagacu ucggaaagug gaggugcuga agguccucgu gaaacauaag      300

cuguguccgc cccugucuga cgaggcucuc aaucgcuggc acacaaagaa agaauacccu      360

cuggaugagg acuuuauucu uuggcagaga accaacgaag ccgauaauaa gaaccccuau      420

caugaccggc acaucugccu gcaucgcaaa cucaaucuga aggauagcca gacggacaga      480

uacacccuug gccgggcccu guaucaccuc acacaacgca gaggguuccu guccaaccgg      540

cuggaucaga augaggaggc ugaagagacc ggaaaaguua agucugccau cagcgaacuc      600

uccaaggaga ugaaagaagc uggcugugag uuucugggcg acuacuucua ccuucuguau      660

agaacagagg ggaaccgcgu gcggauuaga agccgcuaca ccgaucggga aaagcacuau      720

cuccaggagu uuaaagaaau cugcagaguc caggagcugg acgaagaccu ugccaaggcg      780

cuggagaaaa cgauuuucuu ucaacgcccu cucaagucuc agcggcaggg agugggcaaa      840

uguacaaugg agaaggggca uuccagaugc gccguuagcc acccccugua cgaagaguau      900

cgccugcucu guuaccugaa uaacauccgg auuaagaccc cuucugauga cagacuucgc      960

ccgcugaaug cugaagaacg ggagaaaauc acaccccucu ucuauaagga gaaaaacuuu     1020

gauuucgaga agauugccaa aaagcuggcu ggaaagaccc cuuucggcug gaucagagac     1080

gccgaagauu ggcccuacca guuuaacuac cggaugaccc aagaggcgcc ugccugcccc     1140

acaacggcuc agcuuaucaa aguguucggg gaagacugga aggccgcucu ggccgagacc     1200

uauacaaugu ccgccaaaca ggauggaacc cucaagacag aggacgaaau uuguaccgau     1260

aucuggaaug uccuguacag cuuuucuucc aaagagaagc ugaaggaauu uggccucaaa     1320

aagcugcaac uugacgaggu gcaggcuaaa gcguuuagcg aaauucgccu gacgagagau     1380

uucgccuccc ucucucugaa ggcuauccgg aaaauucugu uuuuccuuag aacaggcgag     1440

gacuauagca aggccaucuu ucucgccaac aucccgacca uuguggggga ggaaaucugg     1500

aaugacauug agcgccgguc cagacugcug uacaaggucg aucucgcucu gcgcccucag     1560

gaagagagag acgccgagga acaucggacc aucgcggagc gcauuaaagc ugccguugcc     1620

gcugaagugc ccgaugcccc uaucgaccuu cuguaucacc ccagcaugau cgaggcuuac     1680

cccgaugcca agagaaacga acgguucgac ugcuaucagc ucggaucucc uaaaacaaau     1740

gcggugcgca acccgauggc caugagaucc cugcacaugg uccggaaggu gauuaaucau     1800

cugcuucgca aacacaucau ugaugagaag accgaaaucc auauugagua cgcuagagag     1860

cucaacgacg ccaauaaacg gcaagcuauc gccgauuggc agcgcgaacu gagcaagaga     1920

cacacagcgu acgcccagaa cauucggcag cuguauaagg cugagacggg ccucguuauc     1980

gaacccacca aagccgacau ccugaaguac caacuuuggg aggagcagca gcaugugugu     2040

cuguauacag ggaaaaagau uggaaucgcu gauuuucucg gcgccaaucc uaaauucgac     2100

auugaacaca ccauccccag aucucgcggg ggagauagca cacaggagaa ccugacccug     2160

ugcgaaucca aguuuaaucg ggacauuaag agagacaaac uuccuacgga gcucgccaac     2220

uacgaagaga uccuggcucg caucgagcac uggaagaaac aaugcuaugc gcugaagcuc     2280

aaacgggaug gcauuagaac ccauucuggc cuggaaaaga gcaucaagga cucccgcauu     2340

cggagacgcc accuugucgg gcuggaguac ggauaucuca aaggcaagua cgaucgguuc     2400

acaaugaaag aagugcccga gggguucagc agacggcagg gagccggcau cgggcugauu     2460

ucuaaguacg cuggccuguu ucuuaaaucc cucuucaagg acccgaagaa uccuaaccgc     2520

agcaacguuu auguggucaa aggagccauc accgccgaau uuagaaagcu guggggccug     2580

cagcaggcug augaggccaa aucucgggag aaucauacac accacuguau cgacgcguac     2640

accauugcuu gcaucgggcc cugugaauau gccgcccucg cugccuacua ucgcuccgau     2700

gaggaguuca aguacggaag acggagagaa aaaccucaau uugaaaagcc cuggccuacg     2760

uucacagagg accugcuuaa acugcaggag gaacuccuga uugugcauca gaccacagau     2820

aagcugggca agcgcgaccg gagaaaaguc aagaccccca gagggaaauu ucuuaccgga     2880

ggcgauagcg cgcgggggag acugcaccaa gagacauauu acggcgcuau caacuacgac     2940

ggaaauauua aguauguggu ucgcaaaccg cucgauuccc ugacggaaaa ggaccucgac     3000

aagaucgugg augagaccgu caugggcauc gugaaagggg ccguugaggc caagggaucu     3060

cugaaagaag cucuugccga cggcauuuac augaacaagg agaaagggau ccugauuaag     3120

cgggugagaa ucuucagcga ugucuccgaa ccuauuaauc ucaagcagcu gcgcgacgug     3180

ucuacaaaag aguauaagag acauauccau cugcggaaag augaaaacua ccuuuuugcu     3240

auuuaugagg gaaucgacaa gaaaaagaau aagaaacaga ccgagacagc caccguggau     3300

uaccuggacg cggccaaguu cuauaaagau agcaagcgca gagacaaagc uaacucccuc     3360

guuggcgauu acagcaagaa gaauaaacug ccccucaagu gcauccugaa aaagcggcag     3420

augguccuuu ucuacgaaca cucucccgag gaaauucgcu ucaaagacaa gaaagaccug     3480

uccagacggc ucuauaucgu gacgagcauu gccaaggaug ggagagugaa guuucugcac     3540

caccaagcug ccacagccac cggcauccag ucuuccccua gcccguucac caacgacgga     3600

gagcaucagc ccuacuaucg ccugucugcu agcaauauua aaguccuugu ggcgggcauc     3660

gauuucgaca ucaacauucu gggggaaauc acaccuaagc ucuga                     3705


<210>  4059
<211>  3711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4059
auguacuucu auaagaacaa agagaauaag cugaacaaaa aggugguccu cggccuggac       60

cuugggaucg ccagcguugg auggugccug accgauauuu cccagaaaga agacaauaag      120

uuucccauca uucuccacgg cgugagacug uucgagacag uggaugacuc ugaugacaag      180

cuccugaacg aaacgcggcg caaaaagaga gggcaacggc gcagaaaucg gcgccucuuu      240

accagaaaac gggauuucau caaguaccug auugacaaca auaucaucga gcuggaauuu      300

gauaaaaacc cuaagauucu cguccgcaau uucaucgaga aguauauuaa cccguuuagc      360

aaaaaucugg agcuuaagua caaauccgug acaaaccugc ccaucggauu ccauaaucuc      420

agaaaggcug ccauuaacga aaaauauaag cuggacaaau cugagcuuau cguucugcuc      480

uacuuuuauc ugagccugcg gggcgcguuc uuugauaauc cugaagacac caaguccaag      540

gagaugaaca aaaacgagau ugaaaucuuc gauaagaaug agucuaucaa aaacgcugaa      600

uuucccauug acaagaucau ugaguucuac aaaaucagcg ggaagauucg cuccacaauc      660

aaucucaagu ucggacacca ggacuaccug aaagaaauca aacagguguu ugagaaacag      720

aacauugauu ucaugaauua ugagaaguuu gccauggaag agaaaucuuu cuuuagcaga      780

auccggaacu acuccgaagg cccuggcaau gaaaagagcu ucucuaagua ugggcuuuac      840

gccaacgaaa auggaaaccc cgagcugauu aucaaugaga aaggccaaaa gauuuauacc      900

aaaaucuuua agacacucug ggaauccaaa aucgggaagu guagcuacga caagaaacug      960

uaucgcgcuc cuaagaacuc uuucuccgcc aaagucuuug auauuaccaa uaaacuuacg     1020

gacuggaagc auaagaacga guacaucagc gaaagacuga aacggaagau ucuccugucc     1080

agauuccuga auaaggauuc uaaaagcgcu guggagaaga uccucaaaga agagaacauu     1140

aaguuugaga accuguccga aaucgccuac aauaaagacg auaacaagau uaaucuuccg     1200

aucaucaacg cguaucacag ccugaccaca auuuucaaga aacaucucau caauuuugag     1260

aacuaccuga uuucuaauga aaacgaccuu uccaagcuga ugagcuucua uaaacagcag     1320

ucugagaaac ucuucguucc caaugagaag ggaagcuacg aaaucaacca gaauaacaau     1380

gugcugcaca uuuuugaugc caucuccaac auccugaaua aguucucuac cauucaagac     1440

cgcauccgga uucucgaggg cuauuuugag uucagcaacc ugaagaaaga ugucaagucc     1500

agcgagaucu acucugaaau ugcuaaacuu agagaguuuu ccgggacaag cucucugucc     1560

uucggagccu auuacaaguu uaucccuaac cucaucagcg agggcuccaa aaauuacucu     1620

accauuagcu augaagagaa ggcucugcag aaccagaaga auaacuucuc ccacucuaau     1680

cuguuugaaa agacgugggu ggaggaccuu aucgccagcc ccacagugaa gcgcucccuc     1740

cggcagacca ugaaccugcu gaaagaaauu uucaaguaca gcgagaaaaa uaaccucgag     1800

aucgaaaaga uugucguuga ggugacaaga ucuuccaaua acaaacauga acgcaagaag     1860

aucgagggca ucaauaaaua ucggaaggaa aaauacgagg agcugaagaa aguguaugac     1920

cuuccuaacg aaaauaccac ccugcucaag aagcuguggc ugcuuagaca acagcagggg     1980

uacgaugcgu auagccuccg caaaauugag gccaacgacg ucaucaauaa gcccuggaac     2040

uacgauauug accacaucgu gccgcggucu auuuccuuug augacagcuu cucuaaccug     2100

guuaucguga auaaacugga uaacgcuaag aaaagcaaug accucuccgc caagcaauuu     2160

auugaaaaga ucuacggaau cgagaaacug aaggaggcua aagaaaacug gggcaauugg     2220

uaucuuagaa acgccaaugg gaaggccuuc aacgauaaag gaaaguucau uaagcuguac     2280

acaaucgaca aucucgauga guuugacaac agcgauuuca uuaaucgcaa ccugucugac     2340

acguccuaua ucaccaaugc ucuggucaac caucuuacau uuagcaauuc caaauacaag     2400

uauucugugg uuagcgugaa cggcaaacag accuccaauc uccggaacca gauugcguuc     2460

gucgggauca agaacaauaa agaaacagag agagaaugga agcggccuga gggauuuaag     2520

ucuaucaaca gcaaugacuu ccugauucgc gaggaaggca aaaacgaugu gaaggacgau     2580

guccugauca aagacagauc cuuuaauggc caccacgccg aggaugcuua cuucauuacc     2640

aucauuucuc aguauuuucg gagcuucaag agaaucgaac gccucaacgu gaauuaccgg     2700

aaagagacga gagaacugga cgaucuugag aagaacaaua ucaaauuuaa ggagaaggcc     2760

uccuucgaca acuuccugcu cauuaaugcc cuggaugaac ugaacgagaa acuuaaucaa     2820

augcgcuuua gcagaauggu uaucacaaag aaaaacaccc agcuuuucaa ugaaacccug     2880

uacucuggga aguaugacaa aggaaagaac acaauuaaga aaguggagaa gcugaaccuc     2940

cuggauaauc ggaccgacaa aaucaagaaa auugaagagu uuuucgauga ggacaaacuu     3000

aaggaaaacg agcugacaaa gcuccauauc uuuaaucacg acaagaaccu guacgaaacg     3060

cugaaaauua ucuggaauga ggucaagauc gagauuaaaa acaagaaucu uaacgaaaag     3120

aauuauuuca aauacuuugu gaacaagaaa cugcaggagg gcaagaucuc cuucaaugaa     3180

uggguuccca uucucgauaa cgacuuuaaa aucauucgca agaucagaua uaucaaauuc     3240

agcucugagg aaaaggagac cgaugagauu aucuuuuccc agagcaauuu ccugaagauu     3300

gaccaacggc agaacuuuuc uuuccauaau acacucuacu gggugcagau cugggucuau     3360

aaaaaccaga aggaucaaua cugcuucauu uccaucgacg cucgcaacag caaauuugaa     3420

aaggaugaga ucaaaauuaa uuacgaaaag cugaagaccc agaaagagaa acuucagauc     3480

auuaacgagg aaccuauccu gaagauuaau aagggggacc ucuucgagaa cgaagagaaa     3540

gaacuguuuu auaucguggg aagagaugag aagccccaga agcuggaaau uaaauacauc     3600

cuuggcaaga aaaucaagga ccaaaaacag auucagaagc ccgugaagaa auauuucccu     3660

aauuggaaga aaguuaaccu gacauacaug ggggagaucu uuaagaaaug a              3711


<210>  4060
<211>  3717
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4060
augaccaagg ugcugggccu cgaccugggg gccgcuagcg ucggaugggc caugaucgag       60

gaagaggaaa aaucccacaa gauucuuggc cuggggucua gaaucauucc cuacgaggga      120

acagaaggca acgauuucgc gaaagggacg ggagagagcc ggaaugcucu ccgcaccaag      180

gccagaacag cccggaaagg cuaugaccgc uaccagcuga gacggaagua ucuuguugau      240

acccugguga agaacgagau gaugccugac gaauaccucc gcaaacugcc gaagaugcaa      300

cugugggagc ucagauccaa agcugugaca accgaaauca caaagcagga gcugggccgg      360

cuuuuucugu ggcucaauca gaaacgcggg uauaagucua gcagauccga ugccaaccug      420

gacaagaaag auaccgagua cgucguggaa guuaagucuc ggcaugagaa aauuaaggaa      480

agcggauuca cgaucggcca guauuuuuac uccgagcuua aaagcaauga auacuuccgc      540

gugaaggaga acaucuuucc cagagaggcu uauauugaag aguucgacgc caucugcaaa      600

caacagaagc ggcaccugac cgauaagcuc auuucucgca ucagaaauga aauuaucuac      660

uaucagcggc cucugaaauc ccagaagggg cuggucagcg uguguagauu ugagggauuc      720

uuuaaaaagg gcauuaacgg gaaagaauac uucguuggac ccaagguggc gccuaagucu      780

agcccccucu uucaaacagu caaaaucugg gagaauauca acuccauucg ccugucugac      840

cggaauggca aggagaucau gcuuucccug gaacagaaaa agugcauuuu caaccaucuc      900

gauaauaacg agaaacugac cccuaaggac cuuuuuauca uuaucggcgu gaagaaagaa      960

aauuguuucg ugaacaagca gcuggagaaa gggauccagg gaaacgucac aaagagcacc     1020

auucucaaau gcuucggcga aaauaacgau uaugacaagu ggcugaagcu ggaccucaau     1080

aucauuguua aaaaggauac guuuggguac cuguaugaca aaaagacagg agagaucauu     1140

ggcgagaaac uuaucaagca cguggauucc gccguggaaa gagagccgcu guaccaacuc     1200

uggcauacca ucuacucuau uaacaauaca gacgaaugua gcgcugcccu gcagaacaaa     1260

uucggguuug auuccgagac cgcugacaaa cuuagcaaua ucgauuucgg aaaggccggc     1320

uuuggcaaca agucugcgaa ggucauucgc aaaauccugc ccuaucucau ggagggggac     1380

gauuacuccc agagcauguc uuaugccgga uucaaucaca gcgacucccu gaccaaggau     1440

gaaaaccuga aacggaagcu ccuggacaag cugagaccua uugagaaaaa uucucuucgc     1500

caacccaucg uggaaaagau ucucaaccag augguuaaug uggucaacgc uaucaucgac     1560

aaauacggca agccugauga gauucggauc gaacuggcca gagagcugaa acagaauaag     1620

gaggaacgca acaagacaga caaagauaug agcaagcggc agagagagaa cggggaaauu     1680

uccaaacgcc uugcugagua uggacuccgg gccacgagaa auaacaucau uaaguggcgg     1740

cuguacgaag agaucgacaa ucaagauaaa aagcugaacg ccaucugcgu guauuguggc     1800

cagcccauua gccucaccga ggcuaucaag gggaaugaag uugacgugga gcacauuauc     1860

ccgaaaucua agcuguuuga ugacucccag agcaacaaaa cacuugcgca ucgcagaugc     1920

aauucuacca agggcgauau gacagccuac gacuucaugg aaaacaaacc ugcucagguc     1980

uuuaaugccu acauugagcg ggugaacaug cuguaugaua auaagaucau cagaaaaguc     2040

aagcgcgaca agcuccugau gcccgaaucc aaaauuccug auaacuucau cgaccggcaa     2100

cugagagaga gccaguacau ugccuccaag gcucgcgaga uccuucagac cauuuguuau     2160

aauguguggu cuacgagcgg aacaguuacc gccagacucc ggcaccugug gggcugggac     2220

gaugcgacca ugucccugca gcuccccaaa uaccgcgaac uggggcuuac agagauggug     2280

gaaugggagu cugagcaugg aaagcaaaaa cacagcaagg aagucaucuc cgacuggagc     2340

aagagagaug accaccggca ucacgcugug gaugcccuga ccaucgccug cacaaaacag     2400

ggcuuuauuc agcgcuucaa cacgcucaau ucuuccaaga ccaaagagga cauggaauuu     2460

gagaucgcua aguguagcau ugaauauaga gagaaccugu cuaaacugga gaaguacauc     2520

auugcccuuc ggcccauccc uguuuccgaa guggaggaug cugucgccaa cauucucauc     2580

agcuucaagu cugggaaaaa gguggcggug aaaggaaagc gcaaaaucgg caagagaggg     2640

aauaagaaaa uuauccagac agacauuauc auuccgcaug gcagccuguc cgaagagagc     2700

guuuauggaa agaucaaaac caucgaacgg gagaagcccc ugaaauaccu cuuccaaaac     2760

ucucagcuga uuuuuaauaa caagaucaag auucuugucg aggaacgccu ggccacauuc     2820

gagaaugaua ccaaaaaggc ucucgccucc cugaaaaagg ugccugugua ccuggacgaa     2880

gagaaaacga ucguccuuga auaugcuacc ugcuuuaagg aggaguacgu gaaaaaguau     2940

gauguugaca caaacuucag caagaccgau aaagugauug acgacaagau caaagaaauc     3000

cuccagcaca gacugggcaa guuuggggga aaagccaagg agaaggaagc guucaaagau     3060

guccaacagg gcgacaagac aauuaaaugg uacgaggaug aggggcugga acggcccauc     3120

cauuccguga gaugcuuuac cggacucucu gccauuguuc cugugaagaa agacgagaau     3180

ggcaucgaua uuggguucgu caagcccggc aacaaucacc acaucgcuau uuauagcgac     3240

aaggauggaa accgcuacga acauaucugu acguuuuggc acgccgugga gcggaaaaag     3300

uaugacaucc cgguugugau uaaagauucc aaugaaaucu ggaacaagau uuggucuaaa     3360

ccugagggca gcuaccccca guccuuccug gacaaacuuc cucaggagaa ucugacacuc     3420

gaucugucua ugcaacagaa cgaaaugcug auccuuggga ugagcuacga ggacauuaag     3480

accgcuaucg acaauuccga uuauaagcag cugagcucua accucuacag acugcagaag     3540

cucgccaacg ccaauuauau guuucgccau caccuggaaa cacaaaucau ugacgauaac     3600

aauuccaaag cuagccggag auucaucaac auucacucuc uuaaggcgcu guuugccugg     3660

aaucccuaca aagucaacau caauugccuc ggagacauuc agaccaacuc ccgcuga        3717


<210>  4061
<211>  3720
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4061
augaccgaga agagaaaauu cgacauccgg gaaguguacc uggccauuca ccauaucguc       60

aaguaucgcg gcaacuuucu caauagcgcu acaguugauu ccuucaaaac gucuaacauu      120

gacuuuacca gccaguucga uagacugaau gagcuuuacc ggcaagugau ccuggaggaa      180

cccuuucaga uuaacaugaa ucagguggac aagaugacaa acaaacuccu ggauaaugac      240

gcccuuaagc uggauaccca gaagcaaguc gcgaaacucc ugccugugau cuauaacgac      300

aagccgguug auaaacagua cacacgccug gcuaccgagu ucuccaaggc cauccucggg      360

uauaaaacaa agcuggacgu gauucuuaau cuggauaccc agaacgccaa ggacugggcu      420

aucagacucg augacgaaga cauugaugac aaacugcccg cccuugucga gaaucuggau      480

gaaucucggc aggagaucgu gacgauuauc cgcgaccucu acgcucaaau uacccugaac      540

gccaucguuc cuaagggaaa aagccugucc gaaucuaugg uggauaagua uaaugaccac      600

aaagaucauc ucgaccugcu uaugggccug auccacgagc ucggggauga cagcgcgaag      660

ggaauuaaac ugagagaggc cuacacacag uacgucggca aguccgggga uaagacccuu      720

aaccaggacg acuuuuaugc ugccaucaaa aagaaucugg augaagacag caaacggucu      780

cccaagauuc agcgccucau cgagcaggcu uccuucaugc cuaaacaaag aacaagcgcc      840

aacggaguga uuccccauca gcugcaccaa cuggaacucg aucggaucau cgagaaccag      900

ggccgcuacu auccuuuucu gaaggaaccc aauucuaaca cccacaagcc caauggcggg      960

aaauacaaac uugacgagcu gguggcguuc cggauuccuu auuacgucgg accccucauc     1020

acgaaacagg aucaggagaa gacauccggc gccgacuuug cuuggaugau uagaaaaccu     1080

gggcaaggag gcgaaaucac ccccuggaac uucgaucagg agguugaccg cauggccagc     1140

gcuaauacau uuauuagacg gaugaccacc aaggauucuu aucugguggc cgaagacgug     1200

cuuccggauu ccagccugau cuacgagaag uucaaagucc ucaacgaacu gaauaugcug     1260

aaggugaacg acagacgccu cuccaucucu cagaaacagg aucuguucaa uaaccuuuuu     1320

aagaaacaaa agacaauucg gacgaagaaa cugcagaccu acaucagaac aaagugggag     1380

cucccuagcg uuaugauuuc cgggcugagc gaccccgaca aauucaauuc uucccuggcc     1440

accuauaucg auuuucgcga cguguucgga gagaaggucg augacccuaa ccggcaggcu     1500

gauuacgaaa agauuaucga guggagcaca guguuugaag acagaaagau uuaucaggcg     1560

aaacuuaauc aacucgauug gcugaccgag ucucagagca aggcccugcu cacgaagcau     1620

uacacaggcu ggggccgccu guccaaaaag cugcuuaccg ggcucaaaga ccagaacgga     1680

gauucuaucc uggaccagcu guggaagacc aaugauaacu ucaugcaaau ccagagccgg     1740

gacgaauuug cuaaacagau ucacgaugag aacgccaagc aauuccauga ggccaauaac     1800

guugacgaca uccuugauga cgcuuuuaca uccccgcaga auaagaaagc cauuagacag     1860

guggauaagg ucgugcagga cgucgugaaa gcgguuggcu augcucccga uaagaucgcc     1920

auugaguuca cccgcagccc ucaagaccgg ccgcagagaa cauuuucucg ccagcggcag     1980

cuccaaucca cguaccugaa cguggccaaa gcucugauga aaagcaaucu caaccaggaa     2040

cugaagucug ucaucgauuc ccagcagacc cuuacagaca agcuguaucu cuacuucacc     2100

caacuggggc aggauaugua cacaggaaag gaaaucaauu uugacgagcu ggugaacuau     2160

cagauugauc acauccuucc ccaagccuuc auuaaagacg acagccucga uaauagaguu     2220

cugguguccg cuccuaucaa caaugccaag ucugacaacg uccccgugaa aaaguucggc     2280

gcgaagaugg gguacuuuug gaaacagcug gccgagaauc accucauuag caagcggaaa     2340

cugaacaauc uuaccacgga uccugacaag aucggaaaau ucaccgcuca gggcuuuauc     2400

caucgccagc ugguggaaac aucccaaguu auuagacucg ucgccaacau ccugggcaac     2460

gaguaugggc acgauggaac cacaauuauc gaagugaccg cuaagaugaa ucaucagaug     2520

cggaaagacu ucgaucugau uaagaucaga gacgugaacg auuaccacca ugccauggac     2580

gcguaucuua cggccuacgu cggcgauuau cucuaccugc gcuaccccaa gcugcggucu     2640

uauuuugugu acggggacuu caaaaagcuc aaagaucaga gccugaagau cagaaauuuu     2700

aacuuccuuc acgaccugac aaaugacgag gcuggagaua aaauuguuga ccaggaaacc     2760

ggcgagauca uuugggauaa gcacgagucc gugaagcaac ucaaaaaggu cuaucauuac     2820

aaauuuaugc uggugagcca ggaaguuuau acacgccagg acgcccuguu caaccagacc     2880

aucuacccgg cuucugaugc cgacaagaga aaacuuauuc cuaucaagaa uaacaagccc     2940

guggaugucu augggggcua cuccggaaau guggacgccu acauggcuau uguucggauc     3000

cacggcaaaa aggaggauaa auauaaggug gucggggugc cuaugcgcgc gguggccgac     3060

cugaaaaagg cugaaagcaa gggaagagau aacuaccuca augccgucca ugagguucug     3120

aaaccccaau uuaccaagaa aaagaaagac cggaagacag gcgaaaucac ggagaugaug     3180

gaugacuucg acgugcuccu ggggaaggug uauuaccgcc agcuuauugu cgauggaaac     3240

aaaaaguuua ugcugggguc uuccaccuau caguacaaug ccaaacagcu cgugcugagc     3300

gacaaggcua ugcaaguucu gucuaaagau gacaaacuua agaaccagga ugagaaucag     3360

aaccugaucg acguguauga ugaaauucuc gagaaggucg accaauacuu cgaacuguac     3420

gauaucaaca aguucagaca gaaacuccac gacgggcgga agaaauuuau ugagcugccu     3480

guggauaaug acuucaacgg caagaaacuu aucagcuaug gaaagcgcgc cacaaucauu     3540

uccauccuga auggccucca cgcgaacgcu accaugagca aucugaagua ccuggggauu     3600

ucuucccccu uuggaaugcu ucagguuccg aacggcauca uucugagccc ugccuccaga     3660

aucugcuauc agucucccac aggccucuuc gaacggaaag ugaagcugag cgaccucuga     3720


<210>  4062
<211>  3738
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4062
augaucauuu acuucaucuu ugagaagauu gugaacacca uccuggucau gaaaaagauu       60

guuggccucg accuggggac aaauagcauc ggaugggccc uuaucaacgc uuauauugau      120

gaauacaaua aagagcaccu guauggcauc gaagccugcg gguccagaau uauccccaug      180

gacgcggcua uucucggaaa cuucgauaag ggcaauucua ucagccagac ggccgaccgg      240

accuccuacc gcgggauuag acggcugcgc gagagacauc uucugcggcg cgaaagacuc      300

caccggaucc uggaucugcu cggauuucug ccuaaacauu auucugacag ccuuaaccgc      360

uacggcaagu uccugaauga uaucgagugu aagcucccgu gggugaaaga cgagacaggc      420

uccuauaagu uuauuuucca agaaucuuuu aaagagaugc uggccgauuu caccgaacac      480

caucccaaac uuaucgcuaa caauaagaag gugccuuacg acuggacaau uuacuaucug      540

agaaagaaag cccucaccca gaagaucagc aaagaggagc uggcuuggau ucugcucaac      600

uuuaaucaga agcgggggua cuaucagcug cgcggagaag aggaagagac acccaacaaa      660

cuugucgaau acuauucccu gaagguggag aaaguugagg auagcggcga aagaaagggg      720

aaggacaccu gguacaaugu gcaccucgag aacggaauga ucuaucggcg cacgucuaau      780

aucccucugg auugggaagg caaaaccaag gaguucauug ucacaaccga ccuugaagcc      840

gacggguccc ccaaaaagga uaaagaggga aacaucaagc ggagcuuucg ggcgccuaag      900

gacgaugacu ggacacugau uaaaaagaaa accgaggccg auaucgacaa gauuaaaaug      960

acggugggcg cuuacaucua cgauacacuc cugcaaaagc cggaccagaa gaucagaggc     1020

aaacugguuc gcaccauuga acggaaguau uacaaaaacg agcucuauca gauccugaag     1080

acacagucug aguuccacga agaacuuaga gauaaacaac uguacauugc cugccucaau     1140

gagcuguauc ccaacaauga gccucgccgg aacuccauca gcaccagaga cuuuugucau     1200

cuuuucauug aagauaucau uuucuaccag cgcccccuga agucuaagaa aucccucauc     1260

gacaauugcc cuuaugagga aaaccgguac aucgacaagg agagcgggga gauuaaacac     1320

gcuuccauca aguguauugc caaaucucau ccccuguacc aggaauuuag acuguggcag     1380

uucaucguga aucuccgcau uuaucggaag gagaccgaug ucgacgugac acaagaacug     1440

cuuccgacgg aggcggauua cgugacccug uuugaauggc ucaacgagaa aaaggagauc     1500

gaccagaagg ccuucuuuaa auauccuccc uucggauuua agaaaacaac cagcaauuac     1560

agauggaacu augucgaaga uaagccuuac cccugcaaug agacacacgc ucagaucauu     1620

acccgccugg gcaaagccca cauccccaag gcuuuccugu ccaaggaaaa agaggaaacg     1680

cuuuggcaua uucucuauag caucgaggac aagcaagaga uugaaaaagc ccugcacucu     1740

uuugccaaca agaauaaccu guccgaggag uucaucgaac aguuuaaaaa uuucccuccg     1800

uuuaagaagg aauacgggag cuacucugcu aaagcgauca agaaacuccu gccccuuaug     1860

cggaugggaa aguauuggag cauugagaac aucgauaacg gcacaagaau uagaaucaau     1920

aaaauuaucg acggggagua cgaugaaaac auccgcgaga gaguucggca gaaggccauu     1980

aaucugaccg acaucaccca uuucagagcu cucccucugu ggcuggccug uuaucuugug     2040

uacgaucgcc acuccgaagu gaaggacauu gucaaaugga agacacccaa agauaucgac     2100

cucuaucuga agucuuucaa acagcauagc cugcggaacc cuauugugga gcaaguuauc     2160

accgagacac ucagaacggu gcgcgacauu uggcagcagg ucggacacau cgaugaaauc     2220

cacauugagc ugggcagaga aaugaagaau cccgccgaca aacgggcucg caugucccag     2280

caaaugauca agaacgagaa uaccaaccuu agaauuaagg cccugcucac agaauuucug     2340

aauccggagu ucggcaucga gaacgugcgg ccuuacagcc ccucucagca ggaucugcuu     2400

cgcauuuaug aagagggggu ucucaauucc auccuggaac ugccugagga caucggaauu     2460

auccucggca aauuuaacca gaccgauaca cugaagagac ccacccggag cgaaauucuu     2520

cgcuacaaac uguggcucga gcaaaaguac agaucuccuu auacggggga gaugaucccc     2580

cuguccaaac uguucacccc ggcguacgaa auugagcaua ucaucccuca gagccgguau     2640

uuugacgauu cccuuucuaa uaaggugauu ugcgaaagcg agaucaacaa gcucaaagac     2700

cgcucccugg gauacgaguu cauuaagaau caccauggcg aaaaagucga gcuggcuuuu     2760

gauaagcccg uggaaguccu cucuguggag gccuaugaaa aacugguuca cgagagcuac     2820

ucccacaaca gaagcaagau gaagaaacuu cugauggagg acauccccga ucaguucauu     2880

gaacggcagc ucaacgacuc uagauauauc uccaaggugg ucaaaagccu gcugucuaau     2940

auugugcgcg aggaaaacga gcaagaagcc aucuccaaga auguuauccc uuguacaggg     3000

ggcauuaccg aucggcuuaa aaaggacugg ggaaucaacg acguguggaa uaagauuguc     3060

cugccgagau uuauccgccu caacgagcug acagagagca cccgguucac gucuauuaau     3120

acaaacaaua ccaugauccc cagcaugccu cucgaacugc agaaaggcuu uaacaagaaa     3180

agaaucgauc aucgccacca ugcuauggac gccauuauca uugcuugcgc caaucggaac     3240

aucgugaauu accuuaacaa uguguccgcg agcaagaaca caaaaauuac cagacgcgau     3300

cugcagaccc uccuguguca caaggacaag acagauaaca augggaacua caaauggguu     3360

aucgacaagc ccugggagac guucacccaa gauacacuga ccgcccuuca gaaaaucaca     3420

gucucuuuua agcagaaucu gcgggugauu aacaaaacca cgaaucauua ucagcacuac     3480

gaaaacggaa agaaaaucgu guccaaucaa agcaagggcg acuccugggc uauuagaaag     3540

ucuaugcaca aagagacagu ccauggggaa gugaaccucc ggaugaucaa gaccguuagc     3600

cugaaugagg cccucaaaaa gccucaggcu auuguggaga uggaucugaa aaagaagauc     3660

cuugccaugc uggaacucgg auaugacacc aaacgcauua agaacuacuu cgaggaaaau     3720

aaagauacau ggcaguga                                                   3738


<210>  4063
<211>  3741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4063
augagacugg uguaccucgc ccuggcucac augaucaagu uccggggcca uuuucuuuau       60

gagggggacc ugaaagccga aaacaccgau guccaggcuc ucuucaagga cuuuguugag      120

gaauacgaua aaacaauuga ggaaagccac cuguccgaga ucacggugga cgcucuuucu      180

auucugaccg agaaggugag caaauccucu cgccucgaaa aucugaucgc ccauuauccc      240

acagagaaga agaacacccu guucggaaau cucauugccc ugagccuugg ccugcaagcu      300

aacuuuaaaa caaauuucca gcucuccgaa gaugccaagc ugcaguuuuc uaaagacacc      360

uacgaggagg aucuugaagg gcugcucgga gaggucggcg acgaauaugc ugaucuguuc      420

gugagcgcca agaaccugua cgacgcgauc cuccuguccg ggauccuuac aguugaugac      480

aauagcacca aagccccucu gucugcuucc auggugaaga gauauaagga gcacaaagag      540

gagcucgccg cuuuuaagcg guucauuaaa gagaagcugc cgaaaaagua cgaagagauc      600

uuuaaagaug acacgaagaa cggauacgcc ggcuaugucg gcgcggacaa gaaacuucgc      660

aagagaagcg ggaaacuggc caccgaagag gaauuuuaca aguaugugaa aggcauucuc      720

aauaagguug agggagcuga uuacuuucug gacaagaucg aucgggagga cuuccugcgc      780

aaacagagaa cauucgauaa cgggucuauu ccccaucaaa uccaccucca ggaaaugcac      840

gccauucugc ggcgccaggg agagcauuau ccuuuucuua aggaaaauca ggacaaaauc      900

gagaagaucc ugaccuuccg gauucccuac uaugugggcc cucucgcuag aaaagggucc      960

cgcuuugccu gggccgaaua caaggcugau gagaagauca cacccuggaa cuucgacgau     1020

auucuggaca aagaaaagag cgcggaaaaa uuuaucacca gaaugacgcu uaaugaucug     1080

uaccucccug aggaaaaggu ccugccgaaa cacucucccc uguaugagac auucaccgug     1140

uacaacgagc ucacaaaggu gaaguauguc aaugaacaag gagaggccaa auuuuucgac     1200

accaacauga agcaggaaau uuuugaccau guuuucaaag agaaccggaa ggugaccaaa     1260

gauaagcugc uuaauuaccu gaacaaagaa uuugaggagu uccgcaucgu gaaucucaca     1320

ggccuggaca aggaaaacaa ggcuuuuaau gccucccugg gcacguauca cgaucuuaga     1380

aaaauccucg acaagagcuu ccuggaugac aaagucaacg agaagauuau cgaagauauu     1440

auccagaccc ugacacucuu cgaggaccgg gaaaugauua gacagcgccu gcaaaaauac     1500

uccgauaucu uuaccacaca gcagcuuaag gagcuggagc ggagacacua uaccgggugg     1560

ggacgccucu cuuacaagcu gaucaauggc auucggaaca aagaaaauaa gaaaacgauc     1620

cuggacuacc uuauugauga cggguaugcc aacagaaauu ucaugcaacu caucaacgau     1680

gacgcucuga gcuuuaagga ggaaauugcc cgcgcgcaga ucauuggaga cguggaugac     1740

aucgcuaaug uugugcauga ucugccuggc ucccccgcca ucaaaaaggg gauucuccag     1800

agcgucaaga ucguggacga gcugguuaaa gugaugggac acaacccuac aaauauuauc     1860

auugaaaugg cccgggagaa ccagaccacc gauaagggca gacgcaacuc ucaacagcgg     1920

cuuaaacugc uccaggacuc ccugaagaau cuggauaacc ccgucaauau caaaaacgug     1980

gagaaucagc aacuucagaa cgacagacuc uuccuguacu auauccagaa uggcaaggau     2040

auguacacag gggaaacccu ggacauuaac aaucucagcc aguaugauau cgaccauauu     2100

aucccgcaag cguuuauuaa ggauaacucu cuggacaauc gcguccuuac acggagcgac     2160

aaaaacagag gaaaguccga ugacgugccu ucuaucgagg uugugcacga aaugaaaucc     2220

uucuggagca agcugcucag cgucaaacug aucacgcagc ggaaguuuga uaaucugacc     2280

aaagccgagc gcggcgggcu uacagagaac gacaaggcug gauucauuaa gagacagcuc     2340

guggaaaccc ggcagaucac aaaacauguu gcccaagugc uggaugcgag auuuaaugcc     2400

aagcacgacg agaacaaaaa ggucauucgc gaugugaaaa ucauuacccu gaagucuaac     2460

cucguguccc aguuccggaa ggacuuuaaa uucuacaagg uuagagaaau caaugauuau     2520

caccaugcuc acgacgccua ccugaacgcu gucaucggca cggcccuucu gaaaaaguac     2580

cccaaacucg cgagcgaguu uguguauggg gaguucaaga aguacgaugu gcgcaaauuc     2640

auugccaagu cugacaaaga aaucggcaag gcuaccgcca aauauuucuu cuacuccaau     2700

cugaugaacu uuuucaagaa ggaggucaaa uuugcugacg gaacaguggu ugaaagaccu     2760

gauauugaga ccagcgaaga cggcgagauc gccuggaaua agcagacaga uuucaaaauu     2820

gugcggaagg uccuguccua uccccaagug aacaucguua aaaagaccga agugcagacg     2880

caugggcuug accgcggaaa gcccagaggc cuguuuaaug ccaacccuuc uccgaaaccc     2940

aagccugaua gcuccgagaa ucucgucggg auuaaacgga accuggaccc caagaaauac     3000

ggaggcuaug cugggaucuc uaauagcuac gcggugcucg uuaaggccau cauugagaaa     3060

ggcgugaaga agaaagaaac aaugguccug gaguuccagg gaaucuccau ucuugaucgc     3120

aucaccuuug aaaaggacaa aagagcuuuc cugcucggca agggguacaa agauauuaag     3180

aagaucaucg agcugccuaa auauagccug uuugaacuua aggacggauc ucggcgcaug     3240

cuggccucca uucucagcac aaacaauaaa agaggcgaga uccacaaggg gaacgagcug     3300

uucgugcccc agaaauucac cacccuccug uaccacgcca agcggauuaa uaacccgauc     3360

aacaaggauc auauugaaua ugugaaaaag caccgcgacg auuuuaaaga gcuucugaau     3420

uacguccucg aguucaacga aaaguauguu ggagcuacaa agaauggcga gagacugaaa     3480

gaagccgugg cggacuuuga cucuaagucc aacgaggaaa ucugcacgag cuuccugggg     3540

gcugucaauu cuaaaaacgc cggccuuuuu gagcugacca gccucggauc cgccagcgau     3600

uucgaauuuc ugggcgugaa gaucccucgg uacagagacu auacacccuc uucccuccug     3660

aaagauagca uucuuaucca ucaauccauu accgggcugu acgagacacg caucgaccug     3720

ucuaagcucg gagaggauug a                                               3741


<210>  4064
<211>  3747
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4064
augaagaaaa uccugggccu cgaccugggg agcgccucca uuggaugggc ugugaucacc       60

gagaacgucg aaaauggcau uacaaagugc gauauccuug ggaugggauc uagaauuauc      120

cccuacgagg gcacggaagg gaaagacuuc accaagggaa caggcgagag ccggaacucc      180

cugcgcacca aagccaagac agcgagaaag ggcuaugauc gguaccagcu ccgcagaaaa      240

uaucugguug acacccuuau caagaaucaa auguacccug augaagcuac acggaaccug      300

ccgaaaauga agcucuggga gcugcgcgac aaagccgugc acucucccgu gagccugaug      360

gagcucggga gacugcuucu guggcucaau cagaagcggg gauauaaguc cucucgcagc      420

gaugccaacc ugggcaaaaa ggacaccgaa uacgucgcua cggugaaauc cagacaugag      480

aagaugaaag aacuugaugu uaccauuggg caguauuuuu accgggagcu gagcgccaau      540

gaguucuuua aggugaaaga aaacaucuuc ccucgcgagg cuuacauuga agaguuugac      600

gccaucugua agacacagca aauugcgcac cccaugaucc ucagaucuga acugauugau      660

aagauccgga augaggucau cuauuaccag cgcccucuga aaucccagaa gggacucgug      720

agcguuugcg aguucgaagg cuuuuggaga auugggaaag acggaaagga guauuucgug      780

ggccccaaag ucgccccuaa gucuuccccg cuguuucagc uugcuaagau gugggaaaac      840

aucaauaaca uuaaaaucgg cagccggacc ggggaggauc ugaagcucac aaccgaacaa      900

aaaugggcca uuuuccagca ucuggacaau aacgagaaac uuacgcccac agaccugcuc      960

aaaauccuga aucugaagaa ggagaacugu uacgugaaca aacagauggg aaagggccuc     1020

aaugggaaca ucaccaaaca cgcuauucug cagugccuug gaggcucuga uaaguaugaa     1080

aaucugcuca aacuggagcu uaagauggug gacggggaua aggaaggaua ccuguaugac     1140

agaaaaacag gcgagguccu caacuccaag augaucaaau uuauugaucc uaugguugaa     1200

caagagcccu ucuaccagcu guggcauauc auuuacagca uccacgacca ggaggaaugu     1260

ucccgcgugc ugcaagccaa guuugauauc gagcagccua uugcggacaa acucgccgcu     1320

aucgauuucu cuaagcacgc cuucggcagc aaauccgcua aggugauucg gaagauccug     1380

cccuaucuua uggacgggga uggauacagc gaagccaugu cuuaugccgg cuacgaccau     1440

uccaauagcc agaccaaaga ggagaaccug cagagaaagc uccuggaccu gcuuaaaccg     1500

auuccuaaaa auucucuccg ccaacccauc gucgaaaaga uucugaacca gauggugaau     1560

guugugaacg cuaucaucga gaaguauggg aauccugaug aaauucgggu cgagcuggcg     1620

agagaacuca agcagagcaa ggaggagcgc aacgacgccg auaaggcuau guccaaacgg     1680

cagagagaaa augacgccau caccaagcgc cuggaggaau uuggacuucg ggccacaaga     1740

aacaauauua ucaaauggcg ccuguacgag gaaauugaua accaagagaa gaagcucaac     1800

gcuaugugcg uguauugugg ccagcccauc ucucugacgg aggcccugcg ggggaaugac     1860

guugauaucg aacacauuau ccccaaaagc aaacuuuucg acgauuccca gagcaacaag     1920

acccucgcgc auagacacug caauaagaac aaaaaugacc ugacagcuua cgauuuuaug     1980

aagggcaagc cugaggccgu guucaacgaa uacgucgagc gggugaaucu gcucuaugac     2040

aaccacauua uccgcaaagc caagagagau aaacugcuua ugaccgaauc uaagauuccg     2100

gacaauuuua ucgaccggca gcugagagag ucccaauaca ucgcuaaaaa ggcccgcgag     2160

auucuccaga caaucuguca ugaugucugg agcaccucug gaacggugac agcugaacug     2220

cggcaccugu ggggcuggga cgauguuacc augaaccuuc agauggccaa auauagagag     2280

cucgggcuga cccaucaggu ggaaugggag uccgagcacg gaaagaauaa gcaugaaaaa     2340

gaggucauua cagacuggac caagcgcgau gaccacagac accaugcggu ggaugcccug     2400

acaaucgcuu gcacgaaaca aggcuucauu cagcgguuua acacccucag cgccgcuaag     2460

acacgcgaag acaugauguu cgauaucgag cagagauccc acgaauuuaa agagaagcgg     2520

ucucugcuug agaaguacau cauuagcgaa caaccccuga aaguugacga gguggaaaag     2580

gccgucucca auauccucau uucuuucaaa agcgggaaga aaguggcggu guggggaacc     2640

cgcaagaucg gcaagagagg guccaaaaag guuauucaga aaaagaucau ugucccucgg     2700

ggcagccugu cugaggaauc cguguaugga aaaaucaaga agcaggagga gaaagauuac     2760

aaguaucugu uugaaaaccc ccagcuuauc uucaauccua caauuaaagc caagguggag     2820

gaacgccucc ugauguuuga cggcgauguc aaaaaggcua ucaccagccu gaagaaaauu     2880

cccaucuacc ucgacaacaa caagacggug guucuggaga aagccauuug ucuuaaggac     2940

gaguucguga ucaaauacuc ugucgauauc aauuucaaca agguggacaa aguuguggau     3000

ccgaagauua aggaaauccu gcaaaauaga cucaaaaagu uugggggaaa agagaaggag     3060

gcuuucaaag acguccagcu ggaagauaag augauuauca agugguauga agacgagggc     3120

cuggagcggc cuauuaaauc cgugcgcugc uuuaccgggc uuagcgccgu ugugcccguc     3180

aagaaagaug aaaacggaaa ugacaucggc uucgugaagc cugggaacaa ucaucacauc     3240

gccauuuaca cagauaccga ggaaaacaaa cagcagcacg ugugcacauu uuggcaugcu     3300

gucgagagaa agaaguauga cauccccguu auuaucaaaa auaccaacga agugugggau     3360

aagauucaau cucagccuga cggcacguac cccgacagcu uccuggagau gcucccgccu     3420

cccaaucuga accuccuggu gaauaugcag cagaacgaga uguuuauccu uggaacaacc     3480

acagaagaua uuaaacuggc gaucgacaag cgggauuaua aaaucauugg cgacaagcuc     3540

uacagacugc aaaagauguc caucaaaccc agcucugggc agauugauau cgucuuccgc     3600

caccaucugg agacccagcu uauugacgau gaaaauucca agaaaagcaa gcgguuuuau     3660

aacgugcaau cccugggagc ccucuucgcu cugaacccuu uuaaaguuag aaucgacaau     3720

cucggcaacc uggugaagga gaaauga                                         3747


<210>  4065
<211>  3762
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4065
augaaggcca gaggcaacuu ccugcaccag gacacccccg ggcucacagc ugccaaugcg       60

aacaugggag augcugugga gaaacugugc gucgcccuug aagacuggug uacgcugcgg      120

aaucucgagu gcaacauccg caagaaucag aaaaacauuc aaaaucugcu ugaaggcaag      180

accguugagg ugagcgccga aaacgcucug aaagaguccu uugauucuag cagagccucc      240

cggaaggaca aggcuacaau ccucucuacc uggauugcca uccagccugg ggaguaccug      300

gaugcgaaac aggccaagga cgcuagcaaa gaaauugcca augugcuguu cggacuccaa      360

ggcaacauga agaaaaucuu uaucgaguuc gaagaggauc agaagauucg ccuggacaau      420

gaugagcagg ucgaggcuuu uuauucccuu uguccggacg augggguggc ccuguucgaa      480

ucuuuucaga gcaucuacuc cgcguauauu cucaugggaa uccugaagaa cgccaauggc      540

ggcagcauuu cuuccugcaa aguugcugcc uacgaccaau auaaggaaga ucuuaaacug      600

cucaaggacc uggugagaga guucgcuagc gaaaaauacg acgaguuuuu ccggggagau      660

aucuuugacu cuuccaaggg auuccgcgcc agaaacuaua aucccgccac agcuaaaggc      720

uacaccaagu acaacagcgu cagaggggcg ucuuaugaug acuuuaagaa agagguggaa      780

aagauuuuca aaggaacagu ugccgaggaa gauauccgcu acguggacau gaugcagaga      840

uucgcugagg gcaccuuucu gcggcgccuc aagacguccg auaacgggag caucccuuau      900

cagcugcauc uugaagagau ggacgccauu gucgagaauc agaaacaauu cuaccccuuu      960

cuggaauccg agaaggaaaa gcucgagucu cuggugaccu ucagaaucag cuauuacgug     1020

ggaccucuua cacagaaaaa cgcccggcug gauaaucaca acaaugcucg cuuugccugg     1080

ucccagagaa aggaaggcaa agagcaugcg cgggucuauc ccuggaacug ggaggaaguu     1140

auugacagaa agcaaagcgc ugccaaauuc auggagcgcc ucaccggcac auguaccuac     1200

auccuggaug aaccugugcu gccgcggugc ucucuccugu acgaggagua uugucuucug     1260

aaugaacuca acggguccaa guggagagac augggaggcg acuggcaccg cuuugauuac     1320

gagacgcgga uggaaauuau cgagaaucug uucaagaaaa agaaagggag cgugaaguau     1380

gccgcugucg aagacuggcu uaaaugcgag aagcauaagu cuugugccga ggugaaagga     1440

ggccaggggg aaacaggauu ugagagcaag cuguccucuu acguuuucuu uaaagaagug     1500

cuccacgucg augagaucga cgaagcugau auucccauga ucgaggagcu gauuuggugg     1560

aacacccugu ucgaagacag aagcauccuc aaggagucca uugaagauaa auauggcgac     1620

cgccugacag augaccagau caaggccauc ugcaaaaagc gguuuaccgg cugggggaga     1680

cuuagcaagg agcugcucug uggaauuaaa acccacacag auaauggccc uuacucuauc     1740

auggacgugc ugcgcgaagg gaaccccaau aacaaugcgc gguccgagac gaugguuuuc     1800

auggagauuc ugagagauga caaguucgga uuugacaaac uuaucgaaga ggaaaacaug     1860

cgccggguga gcgaggucga uaacuucgac gugaaugauc ucccuggcuc ucccgccguc     1920

agacggaccg ugaaccaggc uaagcgcauu guugacgaga ucguggggau ugccggcugc     1980

gaaccgcaaa auaucuuuau cgaggucaca agagaugaag acggacggaa caaaggcaga     2040

cgcaccaagc ggagauauga uaauauuaag aacgcuaucg ccgcgcugaa aaaggagcag     2100

aaagaauacu ucaauaagga cguguccgau agccugaaca aaacaaaucc uuccgaccuc     2160

gauaacgagc gccuggcccu uuacuuuaug cagaauggga agugcaugua uuguggaaag     2220

gaccuggaua uuaaacagcu cgacaaguac caaguugacc auauccugcc ccagucuuau     2280

gugaaagaug acagcuucga gaacaaggcu cugguccaca uggaagauaa ucagagaaaa     2340

uccgccucua ugcuucucga cgauagcauu auccggaaga ugaagccuua cugggaggcu     2400

cugcgcgccg ccggccugau cggggagaaa aaguuuaaaa accucaccag agugcgggug     2460

uccgacaagg aacugggagg cuucauugcu cgccagaugg uugaaacgag ccaaucuaug     2520

aaacuuacau cccugguccu cgagaagacc uaucccaaua ccaacgugag acccgugaaa     2580

gcgggguuua gcucugaucu gcggaagcgc cuggaccuua ucaagugcag agagauuaac     2640

gauuaccauc acgcccacga cgcuuaucuc gccugugaaa ucggccaguu cauuacaaug     2700

cggcauaaua aacuguacga gaacccuauc uccuacagcc acgccgucaa ggauuuuauc     2760

gcuaaacgcg ccaaguaugc gaaugaccau ggaaccccga ugucuacaua cggcaugccc     2820

gggagcucca gcuucgugau uucuuccuuu cugacgagcg gauucgacga agagaccggc     2880

gaaaucuuca aagaugcuug guccgccgag guugccgcug ugcggauucg ggagucucuc     2940

aacagaaagg acugcuauau cagccgcaug ccugaagaga cauccggggc cuuuuggaag     3000

gaaacccuga uuucuccccg ggauccuagc cuugggaaaa aucugucccu ccccaucaag     3060

aaaggccugg acccgagaaa guacgggggc uauaacgagc agaaauucgc guacuuuuuc     3120

gucuaugagg cugugaagau caagaaaaag ggagaaguua aagaguacgu guuugccgcc     3180

gucccugugc agguugcugc cauuguggcu cgcgauccca augcccugac aggcuucgcg     3240

aagaaagacg ccgaagcucg gaagaucaag uuucuuagaa uuauccgcga gaaagucuac     3300

aaguaucaac ucauugaagu gaacggggag cggcuguaca ucaccggacu gaaaucugug     3360

agaaaugccc gccagauugc ucucagccag gauaugacga ccgucuuccu gaaggccauc     3420

aacgacaaag auguugccga ggacgaacuu aagucccugu uugaggaaau cgugcaacgg     3480

uauagacucu gugcuaaucg gcugggcaac cagcugaaga uuuucagccu uucugauucc     3540

uuuaauagcg cgaacucuga guccaaaaag cgcgcccugg ugagccucgu cgcuaucauu     3600

gacgccaaaa caaauaagau cgaucugucu gggauuggau ccggcaaagc cguggggaac     3660

augagacuca gcuucuccaa ggaaaugagc aacucuaaaa aucacuucgc uuuuaucgac     3720

cagagcguua ccggcauguu cgagcggaag acauaccugu ga                        3762


<210>  4066
<211>  3777
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4066
augaccgagg aaaaccccga caucagaaag guguuccugg ccauucacca uauccucaaa       60

aaucggggcc acuuucugcu ucaggggcaa uccuucaagg agggaaaccu gaauaaccuc      120

auuaaagaac ugcuuaaucu ggauauccuc cauauuucca ucgagaucac agaagagaac      180

auuaauaaga ucacggagau ugcucuggag aaaaagaccc ugacagacaa ggucaacgau      240

cucaaaaaga ucuacccuaa agagaagcag cugcuugaag ccuuucgccu gauuuucggc      300

gggaaaaccu cucucgacaa gcuguuugcg aucgaugagu auaaggagcu ugacgcuagc      360

guuaaauccg ugucuuucaa ggagaaaauu uacgaagaag ugagacacga uuaugagcag      420

guccugagca auuacaucga acuccuggac cuggccaagc ucguguauga uuccaucauu      480

cugucugaca ucaaaaagga gggaaaaaca cuuagcgagu ccaagguuga acuguaccau      540

aagcaccggg aggaucucac caaacugaag acacuuguga aaaacgacag caagcugucu      600

gaagauaaaa aggucgagcu cuauuccgcc auuuuuaagg aagacaaaga caagggcacc      660

aauuacguga acuacauccg caaaagcgag gaggggaagg gaugcaauua ugaagauuuc      720

aaaaaguuuc ugguuaagga gcuggcuaaa cucgaagagu cuuccgccaa ggaagagauu      780

cugagagacc uugagcugga acucuuccug ccgcuucagc ggacgaaaga uaacagcgug      840

guccccuacc aaauccacaa ggaggaacug gugaaaaucc ucgacaaugc ugccaaguau      900

cauaaguuuc ugaacgagaa agaugacucu ggcuacucca uuaucgagaa ggugauucag      960

cugcucgagu uccgcauccc uuauuacguc ggcccccuga acagcuccaa aaaggcgaaa     1020

guugaugggu uugccugguc ugugagaaau aagggauaug aaaacacccc ugugacaccc     1080

uggaauuacg aaaaagucau ugacgagagc gcuuccgccg aaaaguucau caccaaccuu     1140

acaaauaagu guaccuaccu gaaaggcgag gaugugcucc cuaagagcuc ucugcuguau     1200

uccgaguucg cccuucucaa cgaacugaau gcucugaaau acgacgggaa ccggaucagc     1260

cucgaggcgc gcaauguuau uaucgaaaag cuguuuaaag agcagggaaa gaaggugacg     1320

aaaacaucua uuaagaaccu ucugaaagcc gaaggcuaua ucgaugggaa gggagagauu     1380

accggcaucg acauuacagu caaaaaugac cucaagagcu acagagauuu caagaaaauc     1440

cugggcgaca aguuuaacac cgagcacgug gaaaauauca uucuguggau cacccuuuau     1500

ggggaguccc ggaaacucau uaaggcuaaa aucgaagccg uuuacggaga uauuuauucu     1560

aaggaggaaa ucgcuaagau gagccgccug guguacaaag acuggggcag auucucccgg     1620

aagcugcuca cagagcuggu cagcaaaaag cuguacaacg aggaaacggg ggagugccuu     1680

aauaucauug gagccaugcg ccagaacaac auccucuuua uggaacugcu ggccgauaga     1740

uucgacuauu cucaacagau uaucgauuac aauaaagagc uucagaagga ggugaccgaa     1800

auuacaccgg agauccucga cgaucuguau gucuccccca gcgugaaacg gucuaucugg     1860

cagaccguua gaauugugga agaacugaag aagaucauug gcugugcucc ugcgaaaauc     1920

uuugucgaaa caacccgcuc caaccaagac aagaaaaagc ccacggauag ccggaaaaag     1980

cagcucgagc uguucuacaa ggccgugaaa aaggacguua aagaucuuga gaaggaaauu     2040

gggucccuga auuuugacaa acucaacgag agacugucua gcguggaacc uuccaagcug     2100

aaggcuaaaa agcucuaucu uuacuauaca cagcugggac gcugcaugua cucuggcgag     2160

accaucgaca ucacccaacu gaauacaacc gccuacgaua uugaccauau cuauccccag     2220

agcaaaguca aggaugacuc cuucacaaac acggugcucg ugaaacggga auacaaugcc     2280

gcuaagaccg auagauaucc gcugggcagc gacauucaga caccuucuaa ccgccgguuu     2340

uggaaguucc uuaaagagaa ggggcugauc accgaugaga aauacaauag acucguucgc     2400

acagaagagu uuuccgacga ggaacugacc ggauucauug cccggcagcu ggucgaaacg     2460

agccaaucug ugaaggcggu ggcuggcauc cuuggggagc ugaaccccaa aaccuccauu     2520

uguuauagca aggccgagaa ugucucuagc uucagacaga acuuuggaaa gaucaaagaa     2580

ggcaaucgca aguccgauaa cacagagcag cucaucaaag ugcgggaaau uaaugacuac     2640

caccacgcca aggaugcuua ucugaacguu guggucggga acguguacga caccaaauuc     2700

acaagaaaug uuuuuaacuu caucaaggau aaaaaggacg gccggaagua cagccucaau     2760

aacguguuuu augagaaugu cucuaacucc aauaacgugg ccugggagcu gaauaaaacc     2820

guucaucagg uggaaaaggu caugaacaau aacaauauuc uugugacgcg cagaacaagc     2880

gagcaaaaag gaggccuguu cgacgcuacc guguacaagg ccaaagucgc ggccaaggcu     2940

aaggaagggg uuuauuaccc ucucaaaaca uccgaucccg uggugaacga cgucaccaag     3000

uauggaggcu uuaccaaagu gaagaucgcc uacuauucua uuuucgagua cacacugguu     3060

aauaaaaagg gggaagagaa gaucacgcgg aucauuccca ucccuauuua cgugagccag     3120

aacaucaaag augacuccac ccugcuugag uuuggaaaga cacagauuaa caucaaaucu     3180

ggcgaagagg ucaaggauuu caaacugaag uauagaaagc ucugcaucgg ggaccuggug     3240

aaaauuaaua acuuuuucaa uuuuaucggc ggaaagacca acgauagcuu cguuuacgac     3300

ggcgcugugc aggucacacu cgauaccgaa uccgagcgcu auauuaaaac gcugucuaag     3360

uaccaaaauu ggaaaaagga aaacaaagac ggggagcuuu gggagagcau cacaaaggaa     3420

aagaauaccg agcuguauaa ccuccuggug gagaaaauga auagcggaau uuucaucaag     3480

aaaaagccga acaaauuuaa ugaacugaac agcgaagaga uucggaauaa cuucucuucc     3540

aucagccuug aggaacaggc caagguucug cucgaggugc ugaaucuccu gaccaacaag     3600

aaaucuaucu uugaucuuaa guccauuaau aucacagcgg ccagaggcaa aauuagcuuc     3660

aaccugucua accuguccca guuuagcauc auugaacagu ccgucgcugg guucuacgag     3720

aaggagauca ccaucauugg agacaaagaa aaugacaugg agaacaauaa cuguuga        3777


<210>  4067
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4067
augaaguaca uccugggccu cgaccugggg guggccagca uuggaugggc ugucaccgag       60

aucaacgaaa aaggcgagcc cguugggcuu auugaugcca auuccgugau cuucaagccu      120

cuggacaacg auaaaggaaa gcucuauaau guggaaagac gggacaaacg cggcucuaga      180

cggauucugc gcagaaagaa gcaccggguc gagcgcacaa aaaugcuucu ggugaacagc      240

uccuuucuca cgaaugaaga gaucgauaac cuguacguug ggaagcugga caauaucuau      300

gaggugagac ucaaaggacu gaaggaacag cuuucuaaaa acgagauugc gcggcugaug      360

aucuacuauu gcaagaaucg cggcuucaag agcaacagaa aaaccgaaga ucucgagauu      420

cugaaguccc uuggcgagaa aaucucugaa aaggacagcg augagaaaaa gcugaaaccg      480

auuaucgcua agaauacaga acucauugag uccaaggggc ugacccccau cgaagucauc      540

uacaugauuc gggagaacga caauagcauc cugggauuua aaaacaagga gggcaauuau      600

aaauucgggu uuaagcgcaa ccaagugauu gaugaaguua gaaaaauccu cggaacacag      660

cagauucugu cuaaggagau cguggacgaa uacaucgaua uucuuuccag ccagcgggac      720

uucucugacg gcccuggggg agauuccaag uacaaaaucg acuauaccaa gcuggccggc      780

aaauguaagu acacaggcga gguccgcgcc gugaaaagcg cucccucuua ugaaauuuuu      840

accaugcucc aaaagcugaa cgauaucaga uacgugaagu ucacguccga ggacaaaauu      900

gagaagaaaa agcugagcaa agaagucauc cauaagcugu augaucucgu uguggagaag      960

aauaaaaccc ugacauacga ccugaucgaa aaguccauug augaggaaaa caucaaacuc     1020

cugaauauuc cuaaacuuuc uaagagcaag uauguggagc ugcggaaauc cuacuuuaag     1080

gagagaaacg acaaugaaaa ccucaccgag gaagagauca augccuucaa cgagaagcug     1140

aauaacgaac gcaugaaaca ggagcuuauu cggaauaacc ugaaggcuua caaagaacuc     1200

aagagcaaau uuaagaagaa uaacaucgau gagaauagaa uuaaagaacu ugggggaauc     1260

uauuuccugg accucgucgc cgagcugcuu acauacucua agaccgauga gaaacuggaa     1320

uauuuugugg agaacguuga acgcuacucc cucuucaagg agcagcggga caucguggaa     1380

auuaucaaaa cguuccccaa cuauacaaag aauggcaacc ugagccuguc ucuugucaga     1440

gagcucaaua agcugcugau ggaggggcac gacuacgaaa gcucccucuc uagccugaac     1500

uauuacauug auaccgacgu ggauugggag aaauuuccua caaucuccga aauugaggac     1560

agccuuucua ccaagaucac caauccgaac guuaaacaua uccuggugau ucuccgcaag     1620

cuguacaaua cacugcuuuu caaauaugga cggcccgaaa agguccaccu cgagcugucc     1680

agagauuuua gcaacgacuu cucuacgcgc aauaagaucc agaaagagca acuggaaaac     1740

aaggugcgga gagaggucgc ggccuuugaa auguacggcg cuaauaaaga uauuguggcc     1800

gggaaggacc gccucuccaa cgaugacuuc guucggauca aacuguggga ggagcagaau     1860

aaggugugca uguauagcgg aagaaccauu gaaaaauacc agcuuacauc cgcugagguc     1920

cagaucgauc auauccugcc uuauucuaag agcuuugaca acuccuacuc uaauaaggug     1980

cucguuuuca gcaacgaaaa ccaagauaaa aaggagcgga cccccuauca guggcugaaa     2040

ggcacagaaa aguggaauga guuuaaacag cgcgugagac ugaaccuuaa uauuuccaac     2100

aagaagaaag agaaucuccu guucgaagac gaggucguga acaaugaauu ucuggagcgg     2160

gaacuccacg ccaccagcua cucuuccaga cuggcgcuua acaucuucca acgccugauu     2220

ccugugagcg acgaggauaa guacgacgag cacggcaaug aaaaaguuaa guaucucuac     2280

aaccggaaug ucaucgccuu ucaggggaaa augacgucua ugcugagaaa ccuguauucc     2340

cuuaauaagu acacacauag cuucgagucu gauacccucg acauugauaa caaggcuuuc     2400

auccugaaag aauuugagau caauaaggac aaccugaaaa ugaccgccua uaacgugaau     2460

acaggacucg aaauuaccag cgagacaaag guggagaaaa acaaguccgg cgaguucaag     2520

acgaucaaag augaacugcu uaagaaagaa cuggacaaga aagagaaucu cauugagauc     2580

agcgauuacu uuaaggacaa gguccuguuc gaucugaaaa uuguggacuu ugaagagauc     2640

gauaccguug aacccgagau uaucucuauc cuuuauaaga ugauuacagc ucugaaagaa     2700

gagguguacu ccaagaaccg cgagaaucac cuccaucaca gccuggacgc cuuccuccug     2760

accaucauga acagauccau gcagaugaaa cuuauuaagu acaaucagcu gaucucuguc     2820

cucaaaaaca aggaaauuga gaucuuuaau gaagagaagg gggaauauau cgacagcaaa     2880

gaguucgccg aggaacugca aaagcagaaa auuaucgaua uugacggaaa cgagcggggc     2940

aucaaguuua augugaacgg gaaaacacac cgccugaauc uuguuaagcc guacgaaaac     3000

uuccuggaug acauuaagaa uaaaaucuuu gauaagagag agaacaauaa acucccuuau     3060

caugugguca aguccaaagu gucuggcgcu cugcacgcgg aaaccauccu cggagagagc     3120

aagggcgaga uuacgaagcg gaucuccguu uucgacauua acuacaaaaa ccuggggaag     3180

aucuuugaua aagacggauc ucagaaggaa auuuaugaga cccuugugaa auggcuggaa     3240

gccaagagca aggauuaccc caaacucaag aauggcaaca ucauuaaaaa ggucaaaauc     3300

guggacggga auaaggauaa acugaucaag cugggaaaca agcgcuaugu ggagaugggc     3360

agaacaaccg ucaaaauucu uguucugaag aaagaggcug aagaggggcu caaguucgcc     3420

uccaucggcc gguacaaaua caaucugcuc aagagcgaaa aggacgugaa cauuucuauc     3480

uggaaagacg ccauucauuu cuguaaggug cgcuauaaua aacugaagga gaacggauac     3540

agacuuaucc acgaacugau cccuggcgag acaauugagc uggaacucaa uaaagggaac     3600

aucuccaagu gccuggucgu gggauuuagc ggcgggaaga uugagaucuc uuccguuauu     3660

ggagaugcuc uugaccugau caaugauaaa aucagcaccc ggauuaagga acgcuauuac     3720

aucacggugu cuacaauuaa auccaucaag aaaauuaaca agaauauccu cggcgacuga     3780


<210>  4068
<211>  3792
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4068
augcacaaga acaaucccga caucagagag cuguuccucg ccauucauca cauccugaaa       60

uaccggggcc auuuucuuuu cgcugggcag caauuuaagg gaagcgaaau uaaagauaac      120

auccgcgagc ugcuccagua uaauucccug aacauuaaga ucgacaucac cgaugaacuu      180

auuaauaaca ucgccgagau ucugcucaaa aagaaaaauu cuacagacaa aaucauugaa      240

uuuaagaaac uguuugcgga ugagaagcag cugaucgagg cucucagacu ggcccuuggc      300

aacaaaacgc agcuggccaa acucuucgac auugaggcuu acaaggaacu ggauaucagc      360

aucaagacca uuuccuuuaa ggaaaagauc uaugaggacg ugcggucuaa uuacgaggaa      420

auucuugggg aguauaucau uguccuggau acagccaagc ucaucuacga cgcuaucauu      480

cugagcgaaa ucaaaauuaa gaacaugacc aucuccgagu cuaaaguuau uagcuaugaa      540

aagcacggaa aggaucugaa acaacucaag cagcugauca aagaggacuc caagcugagc      600

aaaaaggaga agcgccagcu guucgaaaau aucuuuguga aagaggauga aaaggaggcc      660

aacuacguga auuacauugg caaaaacaau gucgggucuu ccaagagcuc uugcuccuau      720

gaagacuuca aaaagagccu caucaaggag cugaaauacu gugaggaauc uaaacuuaag      780

caggacauuc uggcggagcu cgaacuggag acauuucugc cuagacaacg gaccaaggau      840

aacuccguga ucccguauca gauucauaaa gaggaacucg agaagauccu gaacaaugcc      900

guucaguacc accccuuccu uaacaauaca gacgaauaug gaauuagcau caaggagaaa      960

aucauucagc ugcucgaauu ucgcaucccu uacuaugugg gcucccugaa caccucucac     1020

aaggucggca augggggauu cagcuggucc gugagaaaca aaggcuacga gcggacggcu     1080

guuacccccu ggaauuucaa caaggugauu aauaaagagg aaagcgccaa gaacuuuauc     1140

gagaaucuua caaaccaaug caccuaccug cucuaugaag auguccugcc uaaacauucu     1200

cugcucuacu ccgaguucac acugcuuaau gaacugaaca gcauuaauua ugggggaaag     1260

cgccucaccg uggagcacag aaaccugauc aucgagcggu uuuucaagca gucugcuccc     1320

aaagccacgg gcaaggaacu gaaaaaguuu cuuaugagcg agggggugau ugacggaaaa     1380

gaugaacucu ccggcuucga caucucuauu aagaacgauc uuaagagcua ccgcgacuuu     1440

aaaaagaucc ugggcgagaa auucgauucc gaaaaggucg agaaaaugau uagauggauc     1500

acacucuuug gggagagcaa gcagcugcuu guugacaaga ucaccaaaga auauggagau     1560

auuuacucuu ccgaggaaau caagaaaauu agcggccuga cauauaagga cugggggcgg     1620

cucucugaga aacugcugac cgaaaucgug uccagccaac uuuacgacga guccaccgga     1680

gaguguuuca auauuaucaa cgcgaugcgc aauaacacac uccuguuuau cgaacugcuc     1740

ggcacgaauu acgauuauuu cgagaagauu aacaccuaca augggaagcu gagaaaagaa     1800

aucacagaga uuaccccuca uauccuugac gaucuguaug ugucuccggc cgucaagcgg     1860

agcauuuggc aagugguuag aaucguggag gaacucaaaa aggucauugg auccgagccc     1920

aaacgcaucu uuguggaaac aacccggucu aacaaggaga aaaagggcga cgauggcaga     1980

aaggacagcc gcaaaaaggc ucugcuggaa cuuuacaaaa agaucaaaac guccgauauu     2040

gccgagcucg agaaggcucu ggggcacaca guugacuucg auaagaaaaa gcaggaccug     2100

accgauaucg acaauaacaa acucaaggcc cggaaacugu aucuuuacua uacccagcug     2160

ggaagaugcc ucuacacagg caaggccauu gaucugaaua accuguucgg gagcaauucu     2220

gaauacgaca ucgaccacau uuauccucag uccaagacca aagauggaag cuggaacaau     2280

cuugugcucg ucaacaagac agagaacucu aaaaagucca auggccugau cagcgaaucu     2340

auccagcgca aaaacaagca uuuuuggcgg uuccugaagg cuaaaaaucu cauuagcgag     2400

gaaaaguaca aaagacugac gcgcaccgag gacuuuacag auggggagcu uuccggcuuc     2460

aucgcgcggc agcugauuga aaccagccag ucugugaagg ucguguccaa aauccuccaa     2520

aaccugaauc ccacaaccga gcuguguuuu agcaaggccg aaaacguuuc cgacuucaga     2580

caggaguuug gaaagauuaa agugggcaag cgcaaaaauc uuacggaaga ggagcagaag     2640

aaagcuaagg aagggcagcu gaucaaaguc cgggaucuca acgaccugca ccaugccaag     2700

gaugccuauc ucaauaucgu gguuggaaac guguaccaca uuaaguucac cagaaauccu     2760

aaaaacuuua uccggaagaa uuucaaaaac cgcgaguaua cacugaauac ccuuuucuac     2820

ucugacaagg ucgaaagaaa cggcgaggug gcuugggaga aagggaagac agaagagauu     2880

gugagcuccg uuaugugcaa caaucugauc aacgucaccc ggagaacguc ugaacaaaag     2940

ggagcccucu augaggcgac aauuuacaaa gcuaaugccg ccaaggaagg cagcuacuau     3000

cccgugaaaa ccaacaauaa gaaccugaaa aaugugacac gcuacggggg cuucaccucc     3060

aucaagauug cuuauuacuc uaucuuuaag uaugauaucc ggaacaaaga caaggucaaa     3120

gagaauauua agaucauucc gaucccuauu uacaucagcc agaacaucaa aaccgaucug     3180

gaccuuauua auuucgccga gaagcagauc aagcugaaaa agggagaaaa cauugagaau     3240

cuccagcuga ucuauaaaaa gcucaacauu ggcgcucugg ugaaaaucga ucauuacccc     3300

uacuauaucg ggggaaagac agacgacaau uucuacguug auggcgccgu gcaaguccuu     3360

cuguccagcg acauugaaag auaucucaaa gaggugguua aguuugugaa cuuccagcgc     3420

gaaaauaagg aggcgaaagu cucuaaggag cacaucuccc uggaagagaa cauuaaacug     3480

uacaacacgc uuguguguaa gauggccacc aaaaucuuua cacacaagaa gaauaacaaa     3540

uauaaugauc ugacccagga caaggcuaaa aacacauuca auaagcucag ccuggaggaa     3600

caaugcaaag uucuccugga aauucuuaac cugcugacca auaagaagac gacauuugau     3660

cucucucuga ucaacgagaa aaccuccaga gugaagugug gguucaaucu ucugaaccag     3720

aaacaguuua gcauuaucga gcagucuguc accggacucu acgaaaagga gaucaaaauu     3780

aucgaagacu ga                                                         3792


<210>  4069
<211>  3795
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4069
augaagaacg ugcugggccu cgaccugggg accaauagca ucggaugggc ccuuauugag       60

gucgaugaaa acaaucugcc caucagaauu aucgcuaugg gcucccggau uaucccucuc      120

ucuacaagcg acaaagagga guuccagcgc gggcugucca ucacgaagaa ccaaucuaga      180

accguuagcc ggacacagcg caaaggauac gauagaaagc agcuuaaaaa guccgaccug      240

aagaaaauuc ucaauaagua uaacaucuuu ccgucugagg aacugcugcg gcuccccaug      300

cuggaucuuu ggaaacugcg cagcgacucc gccaauccug augagaacau uagcgcggag      360

cagcucggca gaauccugua caugcuuaau caaaagcggg gguauaaauc ugcucgcucc      420

gaagccaacg ccgacaagaa ggauagcgac uacgugcagg cugugaaagg aagauaugau      480

cagcugaagg accggaaaca gaccauuggc caauacuucu augaugagcu cuacaaugcc      540

aacgcuaaua acucuuacuu ucgcaucaag gaagaggucu aucccagaga ggccuacauu      600

gaagaguucg acacaaucau cuccgcgcag cgggaaaaac acgacuuucu gaccgaugag      660

gugguucauc agcugcgcaa ugaaauuauc uauuuccaga gaaagcucaa aagccaaaag      720

ggccuggugu cuauuugcga guuugagggg uucgaaaaga cagucuuuga caaagagaag      780

aacaaaaaca aggugguuuu cgugggaccu aaagucgccc ccaagaccuc cccucuuuuu      840

cagcuguguc ggaucuggga aguggugaau acgauuaccc ucaagaucaa aaacccggag      900

ggcagcaagu acaaaugggg agaaaagauc cccgauauua aagagaagga gauccuggcu      960

gacuaucuuu ucaagaauga aucccugucu uuuagcaaac uccuggagau ucuggaacuc     1020

aagaaagaga acgucuacgu uaauaagcag auccugaaag ggauuaaggg caacgaaaca     1080

uauuccgccc uucacagcau ccugggggau aaugagcucc ugaacuucga cgugucuauc     1140

augaccucca aucaaacagc ccuucuggug gauagaaaga ccggagagau ucucgaggag     1200

cgcgcuggcc uggaacugga cgcgagccuc gagaaagagc cucuguacca gcuuuggcau     1260

acgaucuacu cucugaagga ucucgacgaa ugcaaaaacg cccugauuaa gcgguucgau     1320

uuugacgacg agacagcuga aaaacugagc agaaucgauu ucaauaagca ggccuuuggc     1380

aacaaaucca auaagucuau gcgcaagauu cuucccuucc ucauggaggg guaugacuac     1440

agccagucca gcucucuggc uggauauaac cacuccaaua gccugaccaa aaacgaacgg     1500

gagcaacaga ucacacucga uagacuggag cuucugccua agaacucucu ccgccagccc     1560

auugucgaga aaauccugaa ucagaugauc aacgugguua augccauucu ggacgaauac     1620

ggcaagccgu ccgaaauccg gguggagcuu gccagagaac ucaaacaaag caaggaugag     1680

cgcaacgaga ccgaccugca aaauuccaag aacaaaaagc ugaaugauga aauugggaaa     1740

cggcucgagg aacugggacu uccugucacc aagagauaua uccagaaaua caaguuuauu     1800

uuccccucuc ugagcaagaa ccucaaagag gcuaagguga auaaccagug uaucuauugc     1860

ggcgaaacau uuaaucugac ggaggcgcug uccggggacg ccuacgaugu ugaccauauc     1920

gugccuaaag cccuucucuu cgaugacucu cagaccaaca agguccuggu gcaccgcaaa     1980

uguaauagca caaagaccaa ccagacagcu uacgauuaua uugccaccaa gggagacgag     2040

caccugaaac ucuacuccga acggcuggac gauugguuug agagaggcau cauuagcuau     2100

ucuaagaugc agcggcuuaa agucucccau aaggaauacc uggagcgcaa aaagcucggc     2160

aaagagacgg aaacagacaa gaagcugugg gagaauuuca ucgauagaca gcugcgggac     2220

acccaauaua uuagcagaaa aucucgcgaa auccuucaga agaucugcaa cgaugugaaa     2280

accacagaag ggggaguuac cgcuaagcuc cggaaccugu ggggcuggga cgaugugcug     2340

augaaucucc agaugcccaa auacaaggaa cuggggcaaa caauuacgaa ggaguggacc     2400

uccgagcacg gaaaaagaaa gcaucagaaa gaagagaucg acaacuggac aaagcgcgau     2460

gaccacagac accaugccau ugaugcgcuu gucaucgcuu guaccaaaca gggcuuuauu     2520

cagcggauca auacacugaa cagcucugac accaaggauc ucaugaucaa ggaaguggag     2580

gaagccaaaa uugaguucaa ugagaagacg acccugcugg aaaaauaucu uaagacacaa     2640

aaacccuuua gcacccagga gguuaugaag gaagccgaca agauccuggu guccuucaaa     2700

gcugggaaga aagucgccac aauuaccaag uucaaagcua cgggcaagaa caaggagaca     2760

ggagugaucg ugccucgcgg cgcccuccac gaacagagcg uuuacgggaa aauuaagguc     2820

aucgagaaag acaagccgcu gaaauaccuc cuggaaaauu cugauaagau ugugaacccc     2880

agaaucaaaa aucuuaucga aauuaagcug uccgagaaca agaaugaccc uaaaaaggcg     2940

cuccuggccc ugaaaaagau ccccauuuuu cuugaugagg aacagagcau cacccuggag     3000

aaagcuagcu gcuauaacga cgccacagug cucaaguaca agcugcaaaa ucucaaagcu     3060

gcccaggucg augacauugu ggaugaaaag guuaaaaaga ucgugcggga gcgccugucu     3120

caguucggaa acaaagaaaa ggaggcguuu aaggacaccc uuugguucaa ugaagagaaa     3180

aagaucccua uuaucaccgu cagacuguuu gcccggcccg augcuaacaa ccuccagaca     3240

auuaaaaagg acgagaaugg caacgggauc ggauucgugg uuacgggcaa uaaccaucac     3300

auugccaucu augaagauaa agacaauaag ccggugcaac auagcuguac cuuuuggcac     3360

gcugucgagc gcaagaaaua caacaucccu gccgugauua gagacuccuc ugaaguuugg     3420

aaugaucugc ugcggaagga gcuucccgaa uccuuccuga acaaacuccc ugccgacaau     3480

cugaaccuca aguuuuccau gcagcagaau gagauguuca uccuggggcu uucucaagau     3540

gaguuuguga aagcuauugc gaacaaugac aagagccugc uguccaaaca ccucuaucug     3600

gucuggagca ucucugaaaa caauuacugg uuccgccauc accuugagac aaagaacucc     3660

gaucugaaga aaaccgacgg cgugaaggaa agcaaaagac ucuauaauau ucggucucug     3720

ggaucccucu uuagccugaa ccccaucaag gugagacuua acuaccuggg cgagauuaca     3780

aaaaucgggg aauga                                                      3795


<210>  4070
<211>  3801
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4070
auggccaagu acauccuggg ccucgaccug gggaccaaca gcauuggaug ggcucuuauc       60

gagguggaug aaaauaacau ucccaucaga auuaucguca ugggcucccg gaucauuccu      120

cugucuagca augaccgcga ggaguuccag agagggcucu ccaucacaaa gaaccaaaau      180

cggacgauuu cucgcaccca gagaaaggga uaugaucgga aacagcugaa gcgcaacgac      240

cuuaauaaag uucugaacaa guacaauauc uuuccgagca agaugcuccu gaaccugccc      300

augcucgauc uguggaaacu uagauccgac ucugccaaua gcaacaagga uauuuccgcg      360

gaacagcugg gccggauccu cuauaugcug aaucaaaaac gcggguacaa gagcgugaga      420

ucugaagcua acuccgacaa aaaggauaca gacuaugugc aggccgucaa gggacgguac      480

gcccagcuua aagaucgcaa ucagaccauu ggccaauauu ucuacgagga gcuguacaac      540

gcuaacguga auaacagcua uuuuaagauc aaagaagagg uuuacccuag agaagccuau      600

aucgaggagu ucgacacaau uaucaauucu cagaagacca aacacgaauu ucucacagau      660

caguucgugc aucagcugcg gaacgagauu aucuacuuuc aacgcaagcu gaaaucccag      720

aagggccuca uuagcaucug cgaguucgaa ggguuugaaa agaccgucuu cgacaaagag      780

aagaauaaac acaagaucgu guuuguugga cccaaagugg cuccuagauc ugcgccccug      840

uuccagcuuu gucgggucug ggaggugauu aacacgaucu ccauuaaggu gaagaauccu      900

gaaggcagca aauauaagug gggagagaga aucccgucca uuaaagaaaa ggagauccug      960

gccgacuacc ucuuuaaaaa cgaaguccug ucuuucacca aacuucugga gauccucggg     1020

cugaauaaga acgauguuua ugugaauaaa caaauucuga agggcaucaa agggaacgag     1080

acauacgcca cccucuacaa uauucuggga gaaaacgagu uccuuaauuu ugacgugagc     1140

aucauucccu ccaaccauac aagcaugcug gucgauaaga aaaccggcga aauccucgag     1200

gaacgcgcug gccuggagcu ugacgugucu cuggagaagc agccuuucua ucagcucugg     1260

cacacgauuu acucccugaa ggaucuggac gaaugcaaaa acgcccucau caagcgguuu     1320

gauuucgacg augagacagc ugaaaaucug agcagaaucg acuuuaacaa acaggccuuc     1380

gggaauaagu cuaacaaaag caugcgcaag auucuucccu uucugaugga gggauaugau     1440

uacucccaau cuuguagccu cgcgggcuau aaucauucca acagccugac caaagacgag     1500

cgggaacagc agguuacacu ggacagacuu gagcuccugc cuaaaaauuc ucugcgccag     1560

cccaucgugg aaaagauucu caaccaaaug aucaaucugg ucaacgccau ucuugaucag     1620

uacggggagc uguccgaaau ccggguggag cucgcuagag agcugaaaca gagcaaggac     1680

gaacgcaaug augccgaccu gcagaacacc aaaaauaaga aacuuaacga ugagaucgga     1740

aagcggcucg cugaacuggg ccugccgauu accaagagau auauccaaaa auacaaguuc     1800

auuuuuccuu cucucuccaa aaaucugaag gacgcccacg uuaacaacca gugcaucuac     1860

uguggggaga cauucaaucu uagcgaagcc cuguccggag augcuuauga cguggaucac     1920

auugucccca aagcgcuccu guuugacgau ucucagacga acaaggugcu gguccaucgc     1980

aagugcaaua gcaccaaaac aaaccagacc gccuacgacu auaucuccac aaagggcgau     2040

gagcaagugc ggaucuacuu cgagagaguu gacgacuggu uuaaaucugg cauuaucagc     2100

uauuccaaga ugcagcggcu uaaagugagc cacaaggaau accucgagcg caagaaaauu     2160

gggaaggaaa ccgagacgga uaaaaagcug ugggaaaauu ucaucgacag acagcugcgg     2220

gauacacaau auauuucuag aaaaucccgc gagauccucc agaaggucug uaacaaugug     2280

accaccacag agggaggcgu uaccgcuaaa cugcggaacc uuugggggug ggacgaugug     2340

cugaugaauc uccagaugcc uaaguacaga gaacugggac agacagucau caaggagugg     2400

acgagcgaac auggcaaacg caagcaccaa aaagaggagg uggaaaacug gaccaagaga     2460

gacgaucacc ggcaucacgc cauugacgcc cuggugaucg cuugcacaaa acagggguuc     2520

auucagcgca ucaauacccu uaacucuucc gauacaaagg accucaugcu gaaggagguu     2580

gaagaggcca aagucaaguu uagcgagaaa accacgcugc ucgaaaagua ccugagaacc     2640

caggagcccu ucucuacaca agaagugaug cgggaggcgg auaaagugcu ugucagcuuu     2700

aaggcuggca agaaaguggc caccauuaca aaguucaaag ccaucggaaa gaauaaagac     2760

uccggcguua uugugccccg cggggcucug caugaacaga gcgucuaugg aaagaucaag     2820

gugaucgaga aagauaagcc ucucaaauac cuguuugaaa acucugacag aauuguugac     2880

ccgaagguga aaaagcuggu cgaggugcgg cuuuccgaga augaaaacaa cagcaagacc     2940

gccaucucca cgcugaaaaa ggcucccauu uuccucaaug aggagaaaac agaaguucug     3000

gaaaaggccu cuuguuauaa cgaugcgacc gugcucaaau acaagcugca gagcaucaaa     3060

gccucccagg cugacgauau ugucgacgag cgcgugaagu uucuugugaa ggagagacug     3120

gcccaauucg gcaauaaaga aaaggaggcu uuuaaagaua uccucugguu caacgaagag     3180

aagcagaucc cuauuaucuc uguccggcug uuugcccgcc ccgacgcgaa uaaccugcag     3240

guuauuaaaa aggaugagaa uggaaagagc aucggguucg ugcuuacagg caacaaucac     3300

cauauugcca ucuaugaaga caaagauaac aagcugaucc aacacuccug caccuuuugg     3360

cacgcugugg agcggaaaaa guacaauauu ccugucguga ucaaaaacuc uagcgacguu     3420

uggucccagc uccuggauaa gcagcucccc gaaagccugc uuaauaagcu gccgaccgac     3480

aaacucaacc ugaaguucuc uaugcagcaa aaugagaugu ucauucuggg gcuuucccag     3540

aaagaauuug acgaggccau caagaacaau cauaagagcu uccugucuaa guaucucuac     3600

cugguguggu ccauuagcaa caacgauuac uauuuuagac accaucucga gacaaagaau     3660

acggaacuga aaaacaucuc uaccgcuaag gaguccaaaa gauacuuccg cuuuaagagc     3720

gucggcgccc uugugggaac aaauccuauu aaaauccggc ugaaccaccu gggcgaaauc     3780

aauaagguug gggaguauug a                                               3801


<210>  4071
<211>  3807
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4071
augaccaaga acauccuggg ccucgaccug gggacaaaua gcauuggaug ggcccuuauc       60

gagguggaug aaaacaauac gcccauuaga aucauugcua ugggcucccg gaucaucccu      120

cugucuacca gcgacaaaga ggaguuccag cgcgggcucu ccauuacaaa gaaccaaucu      180

agaaccguca gccggacaca gcgcaaagga uacgauagaa agcagcugaa aaaguccgac      240

cuuaagaaaa uccugaauaa guauaacauu uuuccgucug aagaacuccu gaaacugccc      300

augcucgauc uguggaaacu ucggagcgac uccgccaauc cuaacgagaa uaucagcgcg      360

gagcagcugg gccgcauucu cuacaugcug aaccagaaaa gaggguauaa gucugcucgg      420

uccgaagcca augccgauaa gaaagacacc gauuacguuc aggcugugaa gggacgcuau      480

gcccagcuua aagagagaaa ccagacaauc ggccaauacu ucuaugagga acuguacaau      540

gcuaacacca auaacagcua cuuucggauu aaggaggaag uguauccccg cgaggccuac      600

acggaagagu ucgacaccau caucaacgcg cagaaaaaua agcacgauuu ucucacagac      660

gaagucgugc aucagcugag aaacgagauu aucuauuucc agcggaaacu gaagucucaa      720

aagggccucg uuuccauuug cgaguuugaa ggguucgaga aaaccguguu ugauaaggaa      780

aagaauaagg acaaagucgu guucguugga ccuaaggugg cccccaagac aagcccucug      840

uuucagcuuu gucgcaucug ggaggucgug aacaccauuu cucugaaaau caagaauccg      900

gaaggcucca aauacaagug gggagagaaa auccccaacc ucaaggagaa gcagauucug      960

gcugacuauc uuuucaaaaa ugauagccug uccuuuacga agcuccugga aauccuggac     1020

cucaacaaaa augaugugua cgucaacaag caaauucuga aagggaucaa gggcaaugag     1080

acauucucua cccuucacac aguucugggg gacaacgaac uccugaauuu cgaugugagc     1140

auuaucccuu ccaaccauac cagcaugcuu guggacaaga aaaccggaga gauccuggaa     1200

gagagagccg gccucgagcu ggaugcuucu cuggaaaagg agccccucua ucagcugugg     1260

cacacaauuu acucccuuaa agaccuggau gaaugcaaga augcccucau caaacgguuu     1320

ggcuucgacg augagacggc ggagaagcug agccauauug acuuuaacaa acaggccuuc     1380

gggaacaagu cuaauaagag caugagaaaa auccugccuu uucuuaugga aggauacgac     1440

uauucccagu cuuguagccu cgcuggcuac aaccacucca auagccugac caaggaugag     1500

cgcgaacaac agauuacacu ggaccggcuc gagcugcuuu cuaaaaacuc ccugagacag     1560

cccaucgucg aaaagauucu caaucagaug aucaacgugg uuaaugccau ccuggagcaa     1620

uaugggaaac ugagcgagau ucgcguggaa cuugcucggg agcucaagca gucuaaggau     1680

gaaagaaacg acgccgaucu gcaaaauucc aaaaacaaga aacugaauga ggaaaucgga     1740

aagcgccucg ccgagcuggg ccuuccgguc accaaacggu acauucagaa guauaaguuc     1800

aucuuuccua gccuguccaa aaaccucaag gacgcucacg ugaauaacca augcauuuac     1860

uguggggaga cauucaaucu gaccgaagcg cugucuggag augccuuuga cguugaucau     1920

aucgugccca aagcccuucu cuucgacgau agccagacga acaagguccu ggugcacaga     1980

aaaugcaacu ccacaaagac caaucagacc gcuuaugacu acaucggcac aaagggcgag     2040

gaacaggucc gcguguacuc ugagcggauu gaugacuggu uuaaaagagg gaucauuagc     2100

uauuccaaga ugcaacgccu gaaaguuagc cauaaggaau accucgagcg gaaaaagcug     2160

ggaaaagaga ccgaaacaga caagaaacuu ugggagaacu ucaucgauag acagcugcgg     2220

gacacgcagu auauuucucg caaggccaga gaaauccucc aaaaggugug uaauaacguc     2280

accacaaccg agggcggggu gacagcuaaa cugcggaauc uguggggaug ggaugacguu     2340

cuuaugaacc uccagaugcc uaaguacaaa gagcugggcc agaccgugau caaggaaugg     2400

acguccgagc acgggaagag aaaacaccag aaggaagaga uugaaaauug gaccaaacgc     2460

gaugaccauc ggcaccaugc caucgaugcg cuggucgugg cuugcacaaa gcaaggcuuu     2520

auucagagaa ucaacacccu caauagcucu gacacaaaag aucugaugca gcgcgagauu     2580

aaggaggcca agaaacuucu gggagaaaac uauaaugaga aggaguucga aaacgacaau     2640

gaguucguga aaaaccacaa ggaaagacuc ucccagcugg augacuaccu gcggcuucaa     2700

aaacccguua aguuugauac caaguauguc gagcaggaag ccgacaaagu gcuggugagc     2760

uucaaggcug gcaaaaaggu cgccacgauc acaaaauuua aggcuaccgg gaagaauaaa     2820

gacacaggag ugaucguucc ccgcggcgcc cuccaugagg cggggcugua cggaauuaga     2880

accaucuacu cuaagggcgg gauugccgaa aaacacauca uuaagaaaua uaaggugggc     2940

gucagcgcuc aggaucacau cuucaccgga aaagagacau acuccgugaa gaaggacaaa     3000

aacggcaacg aaaagguuga ugacaaaauu aagaucgugc ucgauagcau cgucgacaau     3060

aaagugaagg agguugugcu gaaccggcuu aaucgcgggu uuaacaauga ggaagauuau     3120

agaauugacg ucaagaaagc ccugaacaau uucaagaacc ucgaugagga cccucugugg     3180

uacaaugaau cuaaaggaau caagauucug uccaucaaac uuaagacggg ccugaaggau     3240

agcguggugc cgcuccggcu gaacgagaaa aaugagguca uuacccucgu uaagacaggg     3300

aacaaucauc acaucgcuau cuaugaagac aacgacggaa aacugauuca guacuccugu     3360

accuuuuggc augccgugga gcgcaagaaa uauaauaucc ccacagugau uaagaacucu     3420

agcgaagucu ggaaucaagu gcuugauaag aaacugccug aguccuuccu caacagacug     3480

cccgcggaca agcugaaccu uaaauuuucu augcagcaga augaaauguu cauccugggc     3540

cuccuggagg aggaauuuga uaaggccauu aaagacaacg auaagagccu ccuguccaaa     3600

uaccuuuacc ugguuugguc uaucgcugag ggggacuauu gguuccggca ccaccuggaa     3660

accaagaaua cggagcucaa gaaaacaaac ggcgccaagg aaagcaaacg cuacuuuaga     3720

cuguccacca agggacuuau cgaucugaau ccugugaaag uccggcucaa ccaucugggc     3780

gagauuacca agaucgggga guauuga                                         3807


<210>  4072
<211>  3810
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4072
augaacaaua gcaucaaguc caaacccgag gugaccauug gccuggaccu cggggucgga       60

ucuguuggcu gggccaucgu ggauaacgaa acaaauauua uccaccaucu ggggagcaga      120

cuuuucuccc aggcuaagac ggccgaggac cggcgcucuu uuagaggagc gcggcgccug      180

auuagacggc gcaaauacaa gcucaaaaga uucaucaacc ugaucuggaa guauaauagc      240

uacuuuggcu ucaagaacaa agaagauauu cuuaauaacu aucaagagca gcagaagcug      300

cacaauaccg ugcucaaccu gaaacuggaa gcucucaaug ccaagaucga cccuaaagcc      360

cuguccugga uucuucauga uuaccugaag aaccgggggc acuuuuauga ggacaaucgc      420

gauuucaacg ucuacccgac agaggaacuc gcuaauuauu uuggagagua cggcuacuau      480

aagggcauca uugacucuaa agaagauaac gacgauaagc uggaggaggg gcuuaccaaa      540

uacaaguuca gcaaccagca uuggcuggaa gagaucaaaa aggugcucuc caaucaaaca      600

ggacugcccg aaaaauuuaa ggaggaauau gagagccugu ucucuuacgu uagaaacuau      660

uccgagggcc cugggagcau uaauucugug ucccccuacg gaaucuauca ccucgacgaa      720

aaggagggca aagucgugca gaaguacaac aauaucuggg auaaaaccau ugggaagugc      780

agcaucuuuc cugacgaaua ccgggccccc aaaaacucuc cuauugcuau gaucuucaau      840

gagauuaacg aacuguccac aauccgcagc uauuccaucu accuuaccgg augguuuauu      900

aaucaggagu ucaagaaggc cuaucugaac aaacuccugg accuucugau caagaccaau      960

ucugagaaac cgauugaugc gagacaguuc aagaaacucc gggaagagac gaucgccgaa     1020

agcauuggca aggagacacu gaaggacguu gaacgcgagg agaaacugga aaaggaggau     1080

cacaaaugga agcucaaagg ccugaaacuu aacaccaaug ggaagaucca auacaacgac     1140

cuguccagcc ucgcuaaauu ugugcauaag cugaaacagc accuuaagcu ggauuuccuc     1200

cuggaagacc aguauacaac ccuggauaaa aucaauuuuc uccagucucu guucgucuac     1260

cuuggaaagc aucugagaua uuccaaccgg guggacagcg ccaaccucaa agaguuuucu     1320

gauagcaaua agcuguucga gagagugcug caagaacaga aggacgaucu uuuuaaacuc     1380

uucgagcaga cggacaagga cgaugaaaag auucuggcuc aaacacacuc ccugucuacc     1440

aaggccaugc uccuggcgau cacacgcaug accaaccuug acaaugauga ggacaaccag     1500

aaaaauaacg auaagggcug gaauuuugaa gccauuaaga acuucgacca gaaauuuauc     1560

gauauuacca agaaaaauaa caaucugagc cucaagcagg acaaacggua ccuggaugac     1620

agauucauca acgaugcuau ucuguccccc ggggucaagc gcauccuucg ggaggccaca     1680

aagguuuuua augcuauccu caaacaauuc agcgaggaau acgacgugac gaaggugguc     1740

auugagcugg ccagagaacu gucugaggaa aaagagcucg agaacaccaa gaauuaugaa     1800

aaacugauca agaagaacuc cgauaaaauu agcgagcgcc uugaagcccu ggacaucgcu     1860

gaggacaaga uugaagauau ccucaaaucu ccuacaaagu ccuacaaagu gcugcugugg     1920

cuucagcagg accacaucga ucccuauagc cagaaggaga uugcguucga ggacauccuc     1980

accaaaacag aaaagaccga gauugaucau aucauuccuu acuccaucuc uuuugacgau     2040

agcuccucua acaagcugcu gguucucgcc gaaagcaauc aagcuaaauc caaccagacg     2100

cccuaugagu ucaucacaag cggaaaugcc ggcauuaagu gggaggacua cgaagccuau     2160

ugucggaaau uuaaggaugg ggacaccucu cugcuugauu ccacccagag aagcaaaaag     2220

uucgcuaaga ugaugaaaac agacaccucu uccaaguacg auaucggauu ucuggcccgc     2280

aaccucaaug acacacggua cgcgacgauu guguucagag acgcucugaa agauuaugcc     2340

aacaaucacc uggucgagga caagccgaug uuuaaagugg uuugcaucaa cggcggcgug     2400

accagcuucc uucgcaagaa uuuugauaag ucuugguacg ccaaaaagga ccgggauaaa     2460

aacauucauc acgcugucga cgccagcauc aucuccauuu ucagcaauga aacaaagacc     2520

cucuuuaacc agcugacaca auucgcugau uauaaacugu uuaagaauac cgacgggucu     2580

uggaagaaaa ucgauccuaa gacgggagug gucaccgagg ugacagacga aaacuggaaa     2640

cagauuagag uucggaauca gguguccgag aucgccaagg ucauuaacaa auguauccag     2700

gauagcaaca uugagcgcaa ggcgagauac ucccggaaga ucgaaaauaa aaccaacauc     2760

ucucucuuca augacacagu guauagcgcc aagaaaguug gcuacgagga ccaaauuaag     2820

agaaagaacc ugaagacccu ugauauccau gaauccgcug aggaaaauaa aaacucuaag     2880

gugaagaaac aguucgucua ucgcaagcug gugaauguga gccuccugaa caaugacaaa     2940

cuggccgauc uuuuugcuga gaaggaggac auucugaugu accgggccaa ccccuggguu     3000

aucaaucucg cggaacagau uuucaacgag uacacggaaa auaaaaagau caagucccaa     3060

aacgucuuug agaaauauau gcuggaucuc acaaaggagu ucccugaaaa auuuucugag     3120

gccuucguca agagcaugcu gagaaauaaa accgcuauca uuuacaacgu ggaaaagaag     3180

guggugcacc gcaucaaaag acuuaagauu cuguccagcg agcucaaaga aaauaagcug     3240

ucuaacguua ucauucgguc caaaaacgag agcgggacaa agcugucuua ucaggacacc     3300

aucaauucca ucgcccuuau gauuaugcgc agcaucgauc ccaccgcuaa gaaacaguac     3360

auuagagugc cccugaacac acucaaucug caccucggag accaggauuu ugaccugcau     3420

aacaucgaug ccuaucuuaa gaaaccuaag uucgucaaau accugaaggc caaugagauu     3480

ggcgacgaau auaaaccgug gcgggugcuc acgucuggga cccugcugau ccacaagaag     3540

gauaaaaagc ugauguacau uuccagcuuu caaaaccuua aagacguuau cgagaucaag     3600

aaucucauug aaacagagua caaagaaaac gacgauuccg accccaagaa gaaaaagaaa     3660

gcuaaucgcu uccugaugac ccucagcaca auccugaacg auuauauucu ucuggacgcg     3720

aaggauaauu uugacauccu gggccucucu aaaaacagaa uugaugagau ccugaauagc     3780

aaacuuggac uggacaagau caccaaauga                                      3810


<210>  4073
<211>  3810
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4073
augaacaaua gcaucaaguc caaacccgag gugaccauug gccuggaccu cggggucgga       60

ucuguuggcu gggccaucgu ggauaacgaa acaaauauua uccaccaucu ggggagcaga      120

cuuuucuccc aggcuaagac ggccgaggac cggcgcucuu uuagaggagu gcggcgccug      180

auuagacggc gcaaauacaa gcucaaaaga uucgucaacc ugaucuggaa guauaauagc      240

uacuuuggcu ucaagaacaa agaagauauc cuuaauaacu aucaagagca gcagaagcug      300

cacaauaccg ugcucaaccu gaaauccgaa gcgcugaaug cuaagauuga cccuaaagcc      360

cucucuugga uccugcauga uuaccuuaag aaccgggggc acuuuuauga ggacaaucgc      420

gauuucaacg uuuacccgac aaaggagcug gccaaauauu uugacaagua cggauacuau      480

aaaggcauua ucgauagcaa ggaagacaau gauaacaaac ucgaggagga acugaccaag      540

uacaaauucu ccaacaagca uuggcuugag gaagugaaga aaguccugag caaucagaca      600

ggccuccccg agaaguuuaa agaagaguau gaaucucugu ucuccuacgu gagaaacuau      660

agcgaggggc cuggaucuau uaauuccguu agccccuacg gcaucuauca ccuggacgag      720

aaggaaggga aaguggucca aaaguacaac aauauuuggg auaagaccau cggaaaaugc      780

aacaucuuuc cugacgagua ccgggcuccc aaaaauucuc cuauugccau gaucuucaac      840

gaaauuaaug agcucuccac aauccgcagc uauuccauuu accugaccgg cugguuuauc      900

aaccaggagu ucaaaaaggc uuaucuuaau aaacugcucg accugcuuau caagacgaac      960

ggggaaaagc cgauugaugc cagacaguuc aaaaagcugc gggaggaaac caucgcggag     1020

ucuauuggaa aagaaacacu caaggacgug gagaaugaag agaaacugga gaaggaagau     1080

cacaagugga aacugaaggg ccucaaacug aacaccaaug gcaagaucca guacaacgac     1140

cuuagcuccc uggccaaauu ugugcauaag cucaaacaac accugaagcu ggauuuccug     1200

cucgaggacc aguaugcuac acuggauaag auuaacuuuc ugcagagccu cuucgucuac     1260

cuggggaaac aucuucgcua uaauaacaga guugacucug ccaaucugaa ggaauuuucc     1320

gauagcaaca gacucuucga gagagugcug caggagcaaa aagacggacu guuuaaacuu     1380

uucgaacaga ccgauaagga cgacgagaag auccucgcuc agacgcacuc ucugagcaca     1440

aaggccaugc ugcucgcgau cacccgcaug acaaaucugg auaacgacga agauaaucaa     1500

aagaacaaug acaagggcug gaacuuugag gccauuaaaa auuucgauca gaaguuuauc     1560

gacauuacca aaaccaacaa uaaccuuucc cugaagcagg auaagcggua ccucgacgau     1620

agauucauca augacgcuau ucugucuccc ggggugaaac gcauccugcg ggaagccaca     1680

aaggucuuua acgcuauucu uaaacaguuc agccaagagu acgaugugac gaagguugug     1740

aucgagcucg ccagagaacu guccgaggaa aaagagcugg aaaacaauaa gaacuauaag     1800

aaacucauca agaaaaauag cgacaagauu ucugagggac ugaaagcccu ugauaucgcu     1860

gaggacaaga uugaagacau ccugaaaucc ccuaccaaga gcuacaaggu ccuccugugg     1920

cugcagcagg aucacauuga ccccuauucu cagaaagaga ucgcguucga agauauccuu     1980

acaaagaccg agaaaacaga aauugaccau aucauuccuu acuccaucag cuuugaugac     2040

uccucuagca acaagcuccu ggugcuggcc gaguccaauc aagcuaaauc uaaccagacc     2100

cccuaugagu ucauuacgag cggcaaugcc gggaucaagu gggaagauua cgaggccuau     2160

ugucgcaagu uuaaagacgg agauacaucc cuccuggaca gcacccagcg gucuaagaaa     2220

uucgcuaaga ugaugaaaac cgauacaucc agcaaguacg acaucggcuu ucuugccaga     2280

aaccugaaug auacccgcua cgcgacaauu guuuuccggg acgcucucaa ggacuaugcc     2340

aacaaucacc ugguggaaga uaaaccgaug uuuaaggucg ugugcaucaa cggcgggguc     2400

acgucuuucc ugcggaaaaa uuuugacaag uccugguacg ccaaaaagga ucgcgacaag     2460

aacauucauc acgcugugga ugccagcauc auuucuaucu ucagcaauaa aaccaagaca     2520

cuuuuugacc agcucaccca auucgcugau uauaaacugu uuaagaacac agacggaucc     2580

uggaaaaaga ucgauccuaa gaccggcguu gugacggagg ucaccgacga gaacuggaaa     2640

cagauucggg ugagaaauca gguuagcaag aucgccgaag agauugauaa auguauccag     2700

gacucuaaca uugaacggaa ggcgcgcuac uccagaaaaa ucgagaauaa gacaaacauu     2760

agccuguuca augacaccgu guauuccgcc aaaaaggucg gguacgauga ccaaaucaag     2820

cggaaaaacc ucaagacacu ggauaucgac gaaucugugg aggagaauaa aaacagcaag     2880

gugaaaaagc aguucguuua uagaaaacuu gucaaugugu cccugcucaa caaugauaag     2940

cuggcugacc uguuugccga aaaggaggau auucuuaugu accgcgcuaa ccccugggug     3000

aucaaucugg ccgaacagau uuucaacgag uacaccgaaa aucggaaaau caagucucaa     3060

aacgucuuug gaaaauauau gcucgaccug acgaaggagu ucccugagaa guuuagcgaa     3120

gcguucguga aauccaugcu cagaaauaag acagccauua ucuacaacgu ugagaaaaag     3180

gugguccauc gcaucaaaag acugaagauu cuuucuagcg aacugaagga gaacaaacuc     3240

uccaauguga ucauucggag caagaacgaa ucuggcacca aacuguccua ucaggauaca     3300

aucaauagcg uugcucugau gauuaugcgc ucuaucgacc ccaccgccaa gaaacaguac     3360

aucagagugc cccuuaacac ccugaaucuc caccuggggg aucacgacuu ugaucuccau     3420

aacauugacg cuuaucugaa gaaaccuaag uucgucaagu accuuaaagc caaugagauc     3480

ggcgacgagu auaagccgug gcgggugcug auuuccggaa gccuccugau ccacaaacgc     3540

gauaagaaac ugauguacau uucuuccuuu cagaaccuua augaccugau cgaaauuaag     3600

aaccucaucg agacagaaua caaggagaau guugauagcg accccaaaaa gaaaaagaaa     3660

gccucccaaa uccugagaag ccucucuacg auucugaacg auuauauccu ucuggacgcu     3720

aaggauaauu ucgacauucu gggccucagc aagaaccgga ucgaugagau ucuuaauucc     3780

aaacuugacc uggauaagau cgcgaaauga                                      3810


<210>  4074
<211>  3822
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4074
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuaucgag       60

guggaugaaa auaacuuccc cauuagaauc auugcuaugg gcucccggau caucccucug      120

ucuagcaaug acaaggauga guuucagcgc gggcucucca uuacaaagaa ccaaucuaga      180

acggucagcc ggacccagcg caagggauac gacagaaaac agcugaagaa guccgaugac      240

uucaaauauu cucuucggcg cauccugaau aaguacaaca uuuuuccgag cgaggagcuc      300

cugaaacugc ccaugcucga ucuguggaaa cuuagauccg acgccguuaa uccuaacgaa      360

aauaucagcg cggagcagcu gggccggauu cucuauaugc ugaaccaaaa acgcggguac      420

aagucugcua gauccgaggc caaugccgau aagaaagaca cagauuaugu gcuugcugug      480

aagggacggu acgcccagcu gaaagaccgc aaccagacca ucggccagua uuucuacgag      540

gaacuccaca augcuaagac aaccaacagc uacuuuagaa caaaagaaga ggucuauccc      600

cgggaggccu acauugaaga guucgauacc aucaucaaug cgcaaaaguc uaaacaugac      660

uuucugacgg acgaaguggu ucaccagcug cgcaacgaga uuaucuauuu ccagagaaag      720

cucaaguccc agaaaggccu ggugagcauu ugcgaauuug agggguucga gaagaccauc      780

uuugauaaag aaaaggacaa aaacaagguc guguucguug gaccuaaggu ggcccccaaa      840

acaucuccuc uuuuucaacu gugucggauu ugggaggucg ugaauaccau cacacucaag      900

aucaaaaacc cggaaggcuc caaguacaaa uggggagaga agauucccaa uaucaaggaa      960

aaagaggcuc uggccgauua ucuuuucaag aacgagaccc ugagcuucaa uaaacuccug     1020

gacauucugg aacucaagaa agagaacgug uacgucaaua agcagaucau uaaggggauc     1080

aaaggcaacg aaacguauuc cgcucugcau agaauccuug gggauaauga gcugcucaag     1140

uuugacguuu cuauuauccc uagcaaccac acauccaugc ugguggauaa aaagaccgga     1200

gaaauucuug aggagcgcgc cggccuggaa cucgacguga gccuggagaa agaaccccug     1260

uaccagcucu ggcauacaau cuacucuauu aaggaucugg acgagugcaa aaaugcgcuu     1320

aucaagcggu ucggcuuuga ugacgagauu gccgaaaagc ugucccaccu cgacuucaac     1380

aaacaggcuu uugggaauaa gagcaacaaa ucuaugagaa agauccugcc uuuccugaug     1440

gagggauaua auuacagcca guccuguacc cuugccggcu auaaccacuc uaacagccuc     1500

accaaagaug aacgcgagca gcaggucaca cuggacaagc uggaacuccu gcccaaaaau     1560

ucccuucggc aaccgaucgu ggagaagauu cugaaccaga ugaucaaugu ugugaacgcu     1620

auucucgagg aauacgggaa gccuagcgag aucagaauug aacuggcccg cgagcugaaa     1680

cagucuaagg augaacggca ugacgccgau cuucaaaauu ccaaaaacaa gaagcucaau     1740

gaggagaucg gaaaaagacu gacggaacug ggccuccccg ucaccaagcg cuauauccaa     1800

aaauacaagu uuauuuuccc uagccugucu aaaucccuua aggacgcuca ggugaacaau     1860

cagugcaucu auugugggga gacauuuaac cugaccgaag cgcucagcgg agaugccuac     1920

gacguugauc acauugugcc caagucccug cuguucgacg auucucagac aaauaaaguc     1980

cuugugcauc ggaagugcaa cagcaccaaa acgaaucaaa cagcuuacga cuauaucgcc     2040

uccaagggcg aggaagaggu ccagauuuac ucugccagag uggaugacug guuuaaacgc     2100

ggcaucauca gcuauuccaa gaugcagcgg cucaaaguua gccacaagga guaccuggaa     2160

agaaagucuc ugaacaaaga gaccgaaacc gacaagaaac ucugggagaa uuucauugau     2220

cggcagcugc gcgacacaca auauaucucc agaaagagca aagagauucu ucagaaggug     2280

uguaacaacg ucaccacaac ggaaggggga gugaccgcua agcugcggga ucucuggggc     2340

ugggacgaua uccugaugaa ucugcagaug cccaaauaca aggagcuuag acaaacaguu     2400

auuaaagaau ggaccucuga gcacgggaag cgcaaacauc agaaggaaga gaucgagaac     2460

uggacaaagc gggacgauca cagacaucac gccaucgacg cgcucgugau ugcuugcacc     2520

aaacagggau uuauccagcg cauuaauacg cugaacucca gcgaaaccaa ggaucugaug     2580

caacaggaga ucaaugaagc caaaaagcuc cugggcgaga acuauaaaga caaggaguuc     2640

gaaaauucua acgaacuugu caagaaucau aaagggagac ugagcaagcu cgaugacuac     2700

cugcggcugc agaaaccuau uaaguucaac acaaaauacg uggaaaagga ggccgauaag     2760

auccuuauuu ccuuuaaagc uggcaagaaa guggccacca ucacaaaguu caaagcuacc     2820

ggaaagaaua aagacacggg cguuaucguc ccgcgcgggg cccugcacga agcgggacuc     2880

uauggcguga gaacaauuua cagcaaaggg ggaaucgccg agaagcacau uaucaagaag     2940

uauaaagugg gcgucucugc ucaggaccau auuuuuaccg ggaaggaauc cuacagcgug     3000

aaaaaggaua agaacggcaa ugagaaaguu gacgauaaga ucaaaguggu ccuggacucc     3060

aucguggauu cuaagguuaa agagauugug cucaaccggc ugaauuucgg auuuagcgac     3120

ggcgaagauu auaagaacaa cgucaagaaa gcccuuaaua acuucaagaa ucuggacgag     3180

uacccccucu gguauaacga aaauaaaggg aucaagauuc uguccaucaa acugaagaca     3240

ggacuuaagg auucuguggu uccucugcgc cucaacgaga gcaaugaggu gauuacccug     3300

gucaaaaccg gcaacaauca ccauaucgcu aucuacgaag acaagaacuc caaacccgug     3360

caacacucuu guacauuuug gcacgccgug gagagaaaga aauacaauau uccugcgguc     3420

aucaagaaaa gcuccgaugu uuggaaccag cuccuggaca aggaacuucc cgagagcuuc     3480

cugaauaacc ucccggaaga caaucugaac cugaaguuuu cuaugcagca gaaugagaug     3540

uucgugcuug ggcuguccca agaggaauuu aaagaggcca uugaaaacaa cgauaagagc     3600

cuccugucua aauaucucua ccuggugugg uccgucagcg agaauaacua uugguuccgg     3660

caucaccuug aaacgaaaaa uucugaccug aagaagaccg agggagcuaa ggaguccaaa     3720

cgccuguaca acaucagaag cguguccagc cucuuuucuc ugaauccugu uaaggugcgg     3780

cuuaaccauc ugggcgaaau uacaaaaauc ggggaguauu ga                        3822


<210>  4075
<211>  3825
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4075
augaccaagc acauccuggg ccucgaccug gggacaaaca gcauuggaug ggccaucauu       60

cagguggaua auaacaauaa cguccccauc caaauuaucg cuaugggcuc cagaaucauu      120

ccucuugacu cuaaugaucg ggaccaguuc cagaaagggc aggccaucag caagaacaaa      180

gaucgcacga ccgcgagaac acaacggaag ggauacgacc gcaaacagcu gaagaagucc      240

gaugacuuua aauauucucu caagaaaauu cuggagaaac uugauaucuu cccgaccgag      300

gaacugauga aacuccccac acuggaccug uggaagcuca gaagcgaugc uguuuccaau      360

auugaagaca ucaccccuaa gcagcugggc cggauucuuu acaugcugaa ccagaaacgc      420

ggguauaagu cugccagaag cgaggccaau gcugauaaaa aggacacaga cuacguggcc      480

gaagugaaag gacgguauac ccaacucaag gauaaaggcc agacgcuggg ccaguacuuu      540

uauaaggagc uuuccgacgc uaaccagaau aacaccuacu accgcgucaa ggagaaagug      600

uaucccagag aagccuacau cgaggaguuc gaugcgauca uuaauguuca aaagagcaaa      660

cauucuuuuc ugacagacga agugauccac ucccuccgga acgagauuau cuauuaccag      720

cgcaagcuga aaagccagaa ggggcugguc ucuauuugcg aguucgaagg auuugaaacc      780

acauauuucg auaagaaaac ccaacaggac aagacgaucu uuacaggccc uaaaguggcc      840

cccagaaccu ccccucucuu ccaguuuugu aagaucuggg agguugugaa uaacauuagc      900

cugaaaacaa agaacccgga agggucuaag uacaaauggu ccgaucggau ccccaccauu      960

gaggagaagc agaccaucgc uaauuaucuu caagaaaacg agaaucugag cuucauugaa     1020

cuccugaaaa uccuucagcu gaagaaagag caggucuacg ccaacaagca gauccucaag     1080

ggcauucaag gaaauacaac guuuuccgcu auccauaaaa uuaucgggaa cucugaacac     1140

cugaaguucg acauugagac caucccuagc aaacauuucg ccgugcuggu ggauaagaaa     1200

acaggagaga uucucgacga acgcgauucc cuggagcuua auagcgcgcu ggaacaggag     1260

cccuuuuacc agcucuggca caccaucuau ucuaucaagg accuggacga augcaaaaag     1320

gcccuuauua agagauucaa cuuugaggag gaaaucgcug agaaacuguc caagauugau     1380

uucaauaaac aggccuuugg caacaagagc aauaaagcua ugcggaagau gcucccuuac     1440

cugaugcugg gcuauaacca aucugaagcc gagagcuucg ccggguacaa uagacgccuc     1500

acaaaggagg aaaaauccaa gaacgucucu gacgagcccc ugcagcuucu ggcuaaaaau     1560

agccuccggc agccgguugu ggaaaagauc cugaaccaga ugauuaacgu ggucaaugcg     1620

aucaucgaga aauauggaaa gccugaagag auuagagugg agcuggcccg cgaacuuaag     1680

caauccaaag augagcggga agacgcugau aagcagaacg gcuuuaauaa aaagcucaac     1740

gagcugguug ccaccaaacu gacggaacuc gggcugccca caaccaagca cuacauccag     1800

aaguauaaau ucauuuuucc ugccaaggac aaaaauugga aggaggcuca aguggccaac     1860

caguguaucu acugcggaga uaccuucaau cuuacagagg cgcugagcgg cgacaacuuu     1920

gaugucgacc auauugugcc caaagcucuc cuguucgaug acucucaggc caauaagguu     1980

cuggugcaca gauccuguaa cagcaccaaa acaaauaaca cggccuacga uuauaucacc     2040

aagaaggggu cucaggcucu uaaugacuac gucgcccgcg uggacgauug guuuaaacgg     2100

ggaaucauuu ccuauggcaa gaugcaaaga cucaaaguca gcuucgaaga guaccaggaa     2160

cgcaagaaaa ucggcaagga gacagaagcu gacaagcgga uuugggagaa cuucaucgau     2220

agacagcugc gcgagaccgc cuauauugcg aaaaaggcca aagaaauccu ggagaaggug     2280

ugccauaaug uuacauccac cgaagggaac gugacggcua aacuccggca gcugugggga     2340

ugggacaacg uccuuaugaa ucugcaacuc cccaaguaca aggagcugga gaaaaagacc     2400

aaacagacau uuacccagcu gaaggaaugg acaucugauc acggcaacag aaaacaccag     2460

aaggaggaaa ucauuaauug gaccaagcgg gacgaucauc gccaccaugc caucgacgcu     2520

cuugugauug ccuguacgca acagggguuc auccagagaa uuaacacacu cagcuccucu     2580

gauguuaaag acgagaugaa gaaagaacug gaggaggaua agaccgugua uaaugaacgg     2640

cugacacucc uggagaacua ccuucuggaa aagaagccuu uuagcaccga ggaaaucgag     2700

aaggaggcgg acaaaauucu cgucuccuuc aaggccggca aaaagguggc uacccugagc     2760

aaauacaagg ccacaggaau caaugaaauc aaaggggugc ugguuccgag aggcccccuu     2820

cacgagcaau cugucuaugg aaagauuaag gugaucgaaa aagauaagcc ucucaaauac     2880

cuguuugaga acuccgacaa gauugugaau ccccugauca aacaucucgu caagacgcgc     2940

cugcuugaaa acgagaauaa cgcucaggcc gcccugguga cccucaagaa uaaaccuauu     3000

cugcugaaca auaagcagac agagauccuu gaaaaagcua gcugcuauaa cgaggcgacc     3060

guucugaagu acaaacucca gucucugaag gccucccaaa ucgacgauau uguggacgaa     3120

aagaucaaau uccucauuaa ggagcggcug agcaaauuug gcaacaagga gaaagaggcu     3180

uucaaggaua uccuuugguu uaaugaaaag aaacagauuc ccaucacauc uaucagacug     3240

uucgcccgcc cggacgccaa caaucuccag gucauuaaga aacacgaaaa ggggaaaaac     3300

aucggauuug ugcugagcgg caauaaccac cauauugcua ucuaugagga uaagaauaac     3360

aaacugauuc agcacaucug ugacuucugg caugccguug aaagaaagcg gaauaacauu     3420

ccugugcuua ucgaggauac cuccacgauc uggaaucacc ugauuaacga ggacuuuagc     3480

gaaucuuucc ucaauaagcu gcccaacgau ucccucaaac ugaaguucag ccuucaacag     3540

aaugagaugu uuauccuggg gcucccuaaa gaacaguccg aggaagcgau uaagucuaac     3600

aauaaaagcc ugcuguccaa gcaccuuuac cuggucuggu cuaucacaga cggagauuau     3660

uucuuucgcc aucaccucga gaccaagaac accgagcuga aaaagauuga cggcagcaaa     3720

gaauccaaga gauaccuccg gcugucuaca aaaagccuug uggaccugaa ccccaucaag     3780

guucgccuga aucaucucgg ggagaucacc aagauuggcg aauga                     3825


<210>  4076
<211>  3846
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4076
auggaggacg auagccagca cgacaucaga gaaauuuacc uggccaucca ucacauggug       60

aaguaucggg gcaacuuccu cgucgagggg acccuggaau ccucuaaugc uuuuaaagag      120

gaugaacuuc ugaagcuccu gggacgcauu acaagauacg agaugagcga gggcgaacaa      180

aacuccgaca ucgagcagga ugacgaaaau aaacuuguug cccccgcgaa cgggcagcug      240

gcugaugccc ucugcgccac gcggggaucu cgcagcauga gaguggacaa ugcucuggag      300

gcccuguccg cugugaacga ucucucucgg gagcagcgcg ccauugucaa ggcgaucuuc      360

gccggccugg aagggaauaa acuugaccug gcuaagaucu uugugagcaa ggaguucucc      420

agcgaaaaca aaaagauucu cggaaucuau uuuaauaaau cugauuacga ggaaaagugu      480

guucaaauug uggacuccgg ccugcuugau gacgaggaga gagaguuccu ggaccggaug      540

cagggccagu auaacgccau cgcucucaaa cagcugcugg ggcgcagcac cucugucucc      600

gauagcaagu gcgccucuua cgacgcgcau agagccaauu ggaaccucau uaagcugcaa      660

cuucggacaa aagagaauga aaaggauauc aacgagaauu auggcauucu ggugggaugg      720

aaaaucgacu ccgggcagcg caagagcguu agaggagaau ccgcuuacga gaacaugcgg      780

aaaaaggcca acguguuuuu caaaaagaug aucgagaccu cugaucucag cgaaacagac      840

aagaaucgcc ugauucacga uaucgaggaa gacaaacuuu uuccuauuca gagagauucc      900

gacaacggcg ucaucccgca ucagcugcac caaaaugagc ucaagcagau uaucaaaaag      960

caggggaaau acuaucccuu ccugcuggau gcuuuugaaa aggacggaaa gcaaaucaac     1020

aaaauugagg gccuccuggc cuuccgggug ccuuacuuug ugggcccccu ugucguuccu     1080

gaggaccugc agaaaagcga uaauucugaa aaccacugga uggugagaaa gaagaaaggg     1140

gagaucaccc ccuggaauuu cgacgaaaug guggauaagg acgccuccgg acgcaaguuc     1200

auugagcggc ucgucggcac agauagcuau cugcuugggg agccuacccu gccgaaaaac     1260

ucucuccugu accaggaaua ugaggugcug aaugaacuca acaauguuag acugagcgug     1320

cgcacgggaa accauuggaa ugacaagcgg agaaugcgcc uuggccggga ggagaaaacc     1380

cugcucuguc agagacuguu uaugaagggg caaacaguca ccaaacgcac agcugaaaac     1440

cugcuucgga aggaguacgg aagaaccuau gaacucuccg gccugucuga ugagagcaag     1500

uucacgucca gccugucuac auacggcaaa augugccgca ucuuugggga aaaguacgug     1560

aaugagcacc gggaccucau ggagaaaauu guugaacugc agaccguguu cgaggauaag     1620

gaaacacuuc ugcaucagcu cagacagcug gagggaaucu ccgaagcgga cugugcccug     1680

cuugucaaca cccacuauac cggcuggggg cggcucagcc gcaaacugcu gacaacgaag     1740

gcuggagagu gcaaaaucuc ugaugacuuu gcccccagaa agcacuccau uaucgaaauu     1800

augcgggccg aggauagaaa ccucauggag aucauuaccg acaagcaacu gggcuucagc     1860

gacuggaucg aacaggagaa ucuuggggcu gaaaacggcu ccucucugau ggaggugguc     1920

gaugaccucc gcgugagccc uaaaguuaag cggggaauua uccaguccau cagacugauu     1980

gaugacaucu cuaaagccgu gggcaagcgc cccagcagaa uuuuucugga gcuugcggau     2040

gacauccagc cuuccgggcg gacaauuagc cgcaaaucua gacuccaaga ucuguaccgg     2100

aaugcuaacc ugggaaagga guucaagggc aucgccgacg aacucaaugc cuguuccgau     2160

aaagaccugc aggaugaccg ccuuuuucug uauuacaccc agcucgggaa ggauauguau     2220

acaggagaag aacuggaccu ggacagacuu agcucugcuu acgauaucga ccauauuauc     2280

ccccaggccg ucacccaaaa cgauuccauu gacaaucggg ugcucguugc ucgcgccgaa     2340

aacgcgagaa aaacggauag cuucacauau augccgcaga ucgccgaccg gaugcgcaau     2400

uuuuggcaga uucugcugga uaacggccuc aucucuagag ugaaguucga gcggcugacc     2460

agacaaaaug aguuuagcga acgcgagaaa gaacgguucg uccagagauc ccuuguggag     2520

acccgccaga ucaugaagaa cguggcuaca cugaugcggc agagauacgg gaauagcgcc     2580

gcuguuauug gccucaacgc cgaacugacc aaggagaugc accgcuaccu gggauucucu     2640

cauaaaaauc gggacaucaa cgauuaucac caugcgcaag acgcccuuug cgucggcauu     2700

gcugggcagu uugccgcuaa cagaggauuc uuugccgaug gcgagguguc cgacggggcc     2760

cagaauagcu acaaccagua ucugcgcgac uaccuccggg gauauagaga aaagcugucc     2820

gcugaggauc ggaaacaagg ccgcgcguuc ggguuuaucg ugggcucuau gagaagccag     2880

gacgaacaga agcgggucaa uccucgcaca ggagaggugg uuugguccga agaggauaaa     2940

gacuaccuca gaaaggugau gaacuaucgg aagaugcugg ucacgcagaa agugggcgau     3000

gacuucgggg cccuuuacga ugagaccaga uacgcugcca cagaccccaa gggaauuaaa     3060

ggcaucccuu uugauggggc caagcaagac accucucugu auggaggcuu cagcuccgcu     3120

aaacccgccu acgcgguucu caucgaaucu aaggggaaaa cacgccuggu gaaugucacc     3180

augcaggagu auagccugcu uggcgaucgg cccuccgacg augaacugag aaaggugcuc     3240

gcuaagaaaa agagcgagua cgccaaagcc aacauucugc uccgccacgu uccuaagaug     3300

cagcugaucc gguauggagg cgggcuuaug gugauuaaau cugcuggaga gcugaauaac     3360

gcccaacagc ucuggcugcc guacgaagag uauuguuacu uugacgaccu gucccagggc     3420

aaggggagcc uugaaaagga ugaccugaaa aagcuccugg auucuauccu cggauccguc     3480

cagugccugu accccuggca cagauucacg gaggaggaac uugcggaccu gcauguggcu     3540

uuugauaaac ugccugagga cgaaaagaaa aaugugauua ccggcaucgu cagcgcccuc     3600

cacgccgaug cuaagacagc caaccugucu auuguuggca ugaccggguc cuggcgccgg     3660

augaauaaca agagcggaua uacauucucc gacgaggaug aauuuaucuu ccaaagcccc     3720

ucuggccuuu ucgagaaaag agugaccgug ggggaacuga agcgcaaagc uaagaaagag     3780

gucaauagca aguacagaac gaacgagaag cggcucccua cacuguccgg agccucucag     3840

cccuga                                                                3846


<210>  4077
<211>  3861
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4077
augaccaaga acauccuggg ccucgaccug gggacaaaua gcauuggaug ggcccuuauc       60

gagguggaug aaaacaauac gcccauuaga aucauugcua ugggcucccg gaucaucccu      120

cugucuacca gcgacaaaga ggaguuccag cgcgggcucu ccauuacaaa gaaccaaucu      180

agaaccguca gccggacaca gcgcaaagga uacgauagaa agcagcugaa aaaguccgac      240

cuuaagaaaa uccugaauaa guauaacauu uuuccgucug aggaacuccu gaaacugccc      300

augcucgauc uguggaaacu ucggagcgac uccgccaauc cuaacgagaa uaucagcgcg      360

gagcagcugg gccgcauucu cuacaugcug aaccaaaaga gaggguauaa gucugcucgg      420

uccgaagcca augccgauaa gaaagacacc gauuacguuc aggcugugaa gggacgcuau      480

gcccagcuua aagagagaaa ccagacaauc ggccaauacu ucuaugagga acuguacaau      540

gcuaacacca auaacagcua cuuucggauu aaggaggaag uguauccccg cgaggccuac      600

acggaagagu ucgacaccau caucaacgcg cagaaaaaua agcacgauuu ucucacagac      660

gaagucgugc aucagcugag aaacgagauu aucuauuucc agcggaaacu gaagucucaa      720

aagggccucg uuuccauuug cgaguuugaa ggguucgaga aaaccguguu ugauaaggaa      780

aagaauaagg acaaagucgu guucguugga agcaaggugg ccccuaagac aucuccccug      840

uuucagcuuu gucgcaucug ggaggucgug aacaccauuu cccugaaaau caagaauccu      900

gaaggcagca aauacaagug gggagagaaa acgccgaacc ucaaggagaa ggaaauccug      960

gcugacuauc uuuucaaaaa ugauucccug ucuuuuacaa agcuccugga gauucuggac     1020

cucaacaaaa augaugugua cgucaacaag cagauccuga aagggauuaa gggcaaugaa     1080

accuucagcg cccuucacac aguucugggg gacaacgagc uccugaauuu cgaugugucc     1140

aucauuccca gcaaccauac cucuaugcuu guggacaaga aaaccggaga aauccuggag     1200

gagagagcug gccucgaacu ggaugccucc cuggagaagg aaccucucua ucaacugugg     1260

cacacaaucu acagccuuaa aguccuggac gagugcaaga augcgcucau uaaacgguuu     1320

ggcuucgaug acgagacggc cgaaaagcug ucucauaucg auuuuaacaa acaggcuuuc     1380

gggaacaaga gcaauaaguc caugagaaaa auucugcccu uucuuaugga gggauacgac     1440

uauucucaga gcuguucccu cgccggcuac aaccacagca auucucugac caaggacgaa     1500

cgcgagcagc aaaucacacu ggaucggcuc gaacugcuuu ccaaaaacag ccugagacag     1560

ccuauugugg agaagauccu caaucagaug auuaacguug ugaaugcuau ccuggagcag     1620

uaugggaaac ugucugaaau ccgcgucgag cuugcccggg aacucaagca auccaaggac     1680

gagagaaacg augccgaccu gcagaauagc aaaaacaaga aacugaauga agagauugga     1740

aagcgccucg cugagcuggg ccuucccgug accaaacggu acauccagaa guauaaguuc     1800

auuuuuccgu cccugucuaa aaaccucaag gaugcgcacg uuaauaacca gugcaucuac     1860

uguggggaaa cauucaaucu gaccgaggcc cugagcggag acgcuuuuga uguggaccau     1920

auugucccua aagcccuucu cuucgaugac ucccaaacga acaaggugcu gguccacaga     1980

aaaugcaacu cuacaaagac caaucagacc gccuaugauu acaucggcac aaagggcgaa     2040

gaacaggugc gcaucuacag cgaacggguu gacgauuggu uuaaaagagg gauuaucucc     2100

uauagcaaga ugcagcgccu gaaagugucu cauaaggagu accucgagcg gaaaaagcug     2160

ggaaaagaaa ccgagacaga caagaaacuu ugggaaaacu ucauugacag acaacugcgg     2220

gauacgcagu auaucucccg caaagcuaga gagauucucc agaaggucug uaauaacgug     2280

accacaaccg agggcgggau cacagccaaa cugcggaauc uguggggaug ggacgauguu     2340

cuuaugaacc uccaaaugcc caaguacaaa gaacugggcc agaccgugau caaggagugg     2400

acgagcgaac acgggaagag aaaacaccag aaggaggaaa uugagaauug gaccaaacgc     2460

gacgaucauc ggcaccaugc gaucgacgcu cuggucgugg ccugcacaaa gcagggcuuu     2520

auucaaagaa ucaacacccu caauucuucc gauacaaaag accugaugca gcgcgagauu     2580

aaggaagcca agaaacuucu gggagagaac uauaaugaaa aggaguucga aaacgauaau     2640

gaguucguga aaaaccacaa ggagagacuc agccagcugg acgauuaccu gcggcuucag     2700

aaaccuguua aguuugacac caaguauguc gaacaagagg cugauaaagu gcuggugucu     2760

uucaaggccg gcaaaaaggu cgcuacgauc acaaaauuua aggccaccgg gaagaauaaa     2820

gacacaggag ugaucguucu ccgcggcgcg cugcaugaac agagcgugua cgggaagauu     2880

aaagucaucg agaaggacaa accccucaag uaccuguucg aaaacuccaa aaagauugug     2940

aaccccaaga ucaaaguucu uguggaggag agacuggccg aaaaugagaa caauagcaag     3000

cucucucugu ccacccugaa aaagaacccu auuuuucuua augaagagaa aaccgagauc     3060

cuggaaaagg cuagcuguua uaacgaugcc acaguccuca aguacaaacu gcagucccuc     3120

aaggcuucuc aggccgacga uauuguggac gaucggguua aauuccuggu gaaggagcgc     3180

cuugcgcaau uuggaaauaa agaaaaggag gccuucaagg acacgcugug gcucaacgaa     3240

gagaaacaga ucccgaucau uaccgucaga cuguuugcuc ggcccgaugc caauaaccug     3300

caggcuauca agaaagacaa gaauggccgc gauauugggu ucgugcuuac aggcaacaau     3360

caccacaucg ccauuuauga ggacaaagau aacaagcugg ugcaacauag cugcaccuuu     3420

uggcacgccg ucgaaagaaa gaaaaauaac guuccugcug ugaucaaaaa uuccucugac     3480

guguggaacc agcuccugga caaggagcuc cccgagagcu uccugaacaa acuuccuauc     3540

gauaagcuga agcugaaauu uucccuccag cagaaugaaa uguucguccu gggacuucug     3600

caagaagacu ucgaggcggc cauugagaac aaugauaaga cacuccuguc uaagcaucuc     3660

uaccuggugu ggagcaucac cgacggcgau uacuuuuucc ggcaccaccu ugaaacgaaa     3720

aacacagagc ugaagaaaac cgaaggggcu aaggagucca aacgcuauuu uagacugagc     3780

accaagggac ucauugaccu gaaucccguu aaggugcggc uuaaccaucu gggcgaaauc     3840

acaaaaauug gggaguauug a                                               3861


<210>  4078
<211>  3864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4078
augaagaccg ugcugggccu cgaccugggg acaaacagca ucggaugggc ccuuauugag       60

cuggaugaaa auaacauccc cauuagaauc gucgcuaugg gcucccggau uaucccucuc      120

ucuacgagcg acaaagagga guuccagcgc gggcugucca ucaccaagaa ucaaucuaga      180

acaguuagcc ggacccagcg caaaggauac gauagaaagc agcuuaaaaa guccuaucug      240

aagaaaauuc ucaacaagua caauaucuuu ccgucugacg aacugcugaa acuccccaug      300

cuggaucuuu ggaagcugcg gagcgacucc gccaacccua augaaaacau uagcgcggag      360

cagcucggcc gcauccugua uaugcuuaau cagaaaagag gguacaaguc ugcucggucc      420

gaggccaacg ccgauaagaa agacacagau uauauucaag cugugaaggg acgcuacgcc      480

cagcugaaag acagaaauca gaccaucggc caauauuucu acggcgaacu caacgaugcu      540

aaugagaaca auagcuacuu ucggauuaag gaagaggugu auccccgcga ggccuacaca      600

gaagaguucg acaccaucau caacgcgcag aaagauaagc acgacuuucu gacggacgaa      660

gucauucauc agcugagaaa cgagaucauu uauuuccagc ggaaacucaa gucucaaaag      720

gggcuggugu ccaucugcga auuugaggga uucgagaaaa ccguuuuuga uaaggagaaa      780

aauaaggaca aaguggucuu cgugggcccu aagguugccc ccaagacaag cccucuuuuu      840

cagcuguguc gcauuuggga aguggucaac accaucacac ucaaaaucaa gaauccggaa      900

gggucuaaau acaagugggg agagaaaauu cccgauguga aggaaaagga gauccuggcu      960

gacuaucuuu ucaaaaacga gucccugagc uuuaccaagc uccuggaaau ucugaaacuc     1020

aauaagaaca auguguacuu caacaaacag auccugaagg gcauuaaggg gaaugagacg     1080

uauuccaucc uucacucuau ccugggagau agcgaacucc ugaacuucga cgucuccauu     1140

aucccuagca aucauacauc uaugcuuauu gauaaaaaga ccggcgagau ccuggaagag     1200

agagccggcc ucgagcugga cacaucccug gaaaaagagc cccucaacca acuguggcac     1260

accauuuaca gcaucaagga ucuugacgaa ugcaaaucug cucugauuaa gcgguuuggg     1320

uucgaugacg agaccgccga caaacucagc agaaucgauu uuaauaagca ggcguucgga     1380

aacaagucca auaaaucuau gcgcaagauc cugccuuuuc ugauggaggg cuacgacuau     1440

agccaguccu guagccuugc cggguacaac cauucuaacu ccuucacaaa agaugaacgg     1500

gagcagcaaa uuacgcucga caagcuggaa cugcucccca aaaauagccu gagacagccg     1560

aucguugaga agauucuuaa ccagaugauc aaugugguga acgcuauucu ggaacgcuau     1620

ggaaagccuu cugagauccg ggucgagcuc gccagagaac ugaaacaguc caaggaugag     1680

cgcaaugaca ccgaucugca aaacagcaaa aauaagaaac uuaacgaaga gaucggcaag     1740

cggcucgcug aacuggggcu gccccucaca aagagauaca uucagaaaua uaaguuuauc     1800

uucccuuccc ugucuaaaaa ucuuaaggac gcccacguga acaaucagug cauuuacugu     1860

ggagagaccu uuaaccugac agaggcccuc agcggcgaug cuuucgacgu ugaucacauc     1920

gugcccaaag cgcugcuguu ugacgauucc cagaccaaua agguccuugu gcaucgcaag     1980

ugcaacucua cgaaaacaaa ucaaaccgcc uaugacuaca uugcuaccaa gggcgacgaa     2040

cagguucggg uguacagcga gagagucgau gacugguuca aacgcgggau caucuccuau     2100

agcaagaugc agcggcucaa agugucucac aaggaauacc uggagcggaa aaagcuggga     2160

aaggaaacag agaccgauaa aaagcucugg gagaacuuua uugacagaca gcugcgcgau     2220

acacaauaua ucuccagaaa aagccgggaa auucuucaga aggucuguaa caauguggcc     2280

acgaccgagg gcgggguuac agccaaacug agaaaccucu ggggauggga cgaugugcug     2340

augaaucugc agaugcccaa guacaaggaa cuuggccaaa ccgucgugaa agaguggaca     2400

ucugagcaug ggaagcgcaa acaccagaag gaagagaucg aaaacuggac caaacgggac     2460

gaucacagac aucacgcuau ugacgcccuc guuguggcgu gcacgaagca gggcuucauc     2520

cagcgcauca auacccugaa cuccagcgag acaaaggauc ugaugcaaca ggaaauuaaa     2580

gaggcuaaga aacuccuggg agagaauuau aacgaaaagg aguucgaaaa uaacaaugag     2640

ucugucaaaa accauaagga aagacuuagc cagcuggacg auuaccuccg gcugcagaag     2700

ccugugaaau uugacaccaa guauguggag caagaggccg acaaaguucu ggucuccuuc     2760

aagagcggca aaaagguggc cacaaucacc aaguuuaaag cuacggggaa gaauaaagau     2820

acaggaguga uugucccgcg cggcgcccuu cacgaacaga acguguacgg gaagaucaaa     2880

guuauugaga aggacaaauc ucugaaguac cucuucgaaa auuccgagaa gaucgugaac     2940

cccaaaauua agaacaucgu cgaaaucauu cugagcgaga augagaacaa uuccaaaaug     3000

gcucucucua cccugaagaa aaacccuauc uuucuuaaug aagagaagac agaucugcuc     3060

gaaaaggcca gcuguuauga guacgugacc guuagaaaau auccccugaa caccauuaag     3120

gcgucccagg ccgacgauau cguggacgag cgggucaaau uccuggugaa ggaacgccuu     3180

acacaguuug gaaauaaaga gaaggaggcu uucaaggaua cgcugugguu uaacgaagaa     3240

aaacaaauuc cuaucaucac cguuagacuc uucgcccggc ccgacgcuaa uaaccugcag     3300

gccauuaaga aagaugaguc uggcaaggag aucggguuug uggucauggg caauaaccau     3360

cacauugcga ucuacgaaga caaagaugga aagcucgugc agcacagcug cacauucugg     3420

caugccgugg agcgcaagaa auauaauauu uccaccguca ucaagaacuc uagcgacgcu     3480

uggaaucaau uucuggacaa cgaacuuccg gagccuuucc ugaauaaacu ccccgccgau     3540

aaccugaacc ugaaguucuc ccuucagcag aaugaaaugu uuauccuggg ccucagccag     3600

gaggaguucg acaaagcucu gaagucuaac gauaaauccc uccugagcaa gcaccuuuac     3660

cugguuuggu cuauuuccga aaauaacuau ugguuuagac aucaccugga gacaaagaau     3720

agcgaccuca aaaagaccaa cggggugaaa gaaucuaagc ggcuguacaa uauccgcucc     3780

cuuggaagcc uguucucccu caacccuauu aaaaucagac ugaaucaccu cggcgagauu     3840

acgaagaucg gggaauacag cuga                                            3864


<210>  4079
<211>  3867
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4079
augagacugu ucgaggaagg ccacaccgcc gaggaccggc gccucaagag aacagcucgg       60

cgcagaauca gccggcgcag aaaccggcug cgcuaccuuc aggccuuuuu cgaagaggcg      120

augacggauc uggacgaaaa uuucuucgcu agacuccaag aguccuuucu ggugcccgag      180

gauaaaaagu ggcaucggca cccuauuuuc gccaaacuug aagacgaggu cgccuaucau      240

gaaaccuacc cgacaaucua ucaucugcgc aagaaacucg cugauucuag cgagcaggcc      300

gaccugagac ugauuuaccu cgcucuggcc cacaucguua aguaucgggg gcacuuucuu      360

auugagggaa agcuguccac cgaaaacaca ucugugaaag aucaguucca gcaauuuaug      420

gugaucuaca aucagaccuu cgucaacggc gagagccgcc ucguguccgc gccccugccu      480

gaaucuguuc uuaucgagga agaacugaca gagaaggcca gcagaaccaa aaaguccgaa      540

aaagugcucc agcaguuucc ccaagagaag gcuaaugggc uguucggaca guuucugaag      600

cucauggucg gcaacaaagc cgacuucaag aaaguguucg ggcuggaaga ggaagcuaag      660

auuacguaug ccagcgaguc uuacgaggaa gaucuugagg gaauccuggc gaaaguuggc      720

gacgaauacu ccgauguguu ucucgccgcu aagaaugucu augacgccgu ggagcugagc      780

accauucuug cugauucuga caaaaagucc cacgccaagc ugucuagcuc caugaucgug      840

cgguucacag aacaucagga ggaccucaaa aaguuuaaaa gauucauucg cgagaacugc      900

ccugaugaau acgacaaucu guuuaagaac gagcagaaag auggcuaugc cggguacauc      960

gcucacgcgg gaaaggucag ccaacugaag uucuaucagu acguuaaaaa gauuauccag     1020

gacaucgccg gcgcugaaua uuuucucgag aaaauugccc aagagaauuu ccugcggaag     1080

cagagaaccu uugauaacgg ggugaucccc caucagauuc accuugccga acugcaggcu     1140

aucauucacc gccaagccgc guacuacccu uuccucaaag agaaucagga aaagaucgag     1200

cagcugguga cauuucggau cccguauuac gucggacccc uuuccaaggg cgacgcuucu     1260

accuucgccu ggcugaaaag acagagcgag gagccuauuc gccccuggaa ccuccaagag     1320

acgguggauc uggaccaguc cgccacagcu uuuaucgaac ggaugaccaa uuucgauaca     1380

uaucugccua gcgagaaggu ucuccccaaa cauucucugc uuuacgaaaa guucauggug     1440

uuuaacgagc ugaccaaaau uuccuauacc gacgauagag ggaucaaggc caacuucagc     1500

ggaaaggaaa aagagaagau uuuugacuac cucuucaaaa cacgccggaa ggucaaaaag     1560

aaagacauca uccaguuuua uagaaaugag uacaacacgg aaauugugac ccugucuggc     1620

cuggaggaag aucaguucaa ugcuagcuuu uccacauacc aagaccuucu caaguguggc     1680

cugacccgcg ccgagcugga ucacccggac aacgcggaaa agcucgagga uaucauuaaa     1740

auccugacaa uuuucgagga ccggcagaga auccggaccc agcuuucuac guuuaagggg     1800

caguucagcg ccgaaguucu gaaaaagcuc gagcgcaaac auuauacagg auggggcaga     1860

cuguccaaga agcugauuaa ugggaucuac gauaaagaaa gcggaaagac cauccuugac     1920

uaucucguga aagaugacgg cgucucuaag cacuacaacc ggaauuuuau gcaacugauu     1980

aacgauuccc agcugagcuu caaaaaugcu auccagaagg cccaaucuuc cgagcaugag     2040

gaaacccuca gcgagacagu gaacgaacug gcuggguccc cugccauuaa gaaaggaauc     2100

uaucagucuc uuaagauugu cgacgagcug guggcgauca ugggcuacgc ccccaaaaga     2160

aucguugugg aaauggcucg cgagaaucag accacaagca cgggcaagcg gagauccauu     2220

cagcgccuca aaaucgucga gaaggccaug gcugaaauug ggucuaaccu gcugaaggag     2280

caaccuacca caaaugaaca gcuuagagau acccggcucu uucuguauua caugcagaac     2340

ggaaaagaca uguacacagg cgacgagcug agccuccacc gccuguccca cuaugauauc     2400

gaccauauua ucccccagag cuucaugaag gaugacucuc uugauaaucu ggugcucguu     2460

ggguccaccg aaaacagagg aaaaagcgac gaugugcccu cuaaggaggu cgugaaagac     2520

augaaggccu acugggagaa acuguaugcc gcuggccuga ucucccaacg gaaguuccag     2580

cgccuuacga agggggaaca gggcggacuc acccuggagg auaaagcgca cuuuauucag     2640

agacaacugg uggaaacacg gcagaucacc aagaacguug ccggcauucu cgaccagcgc     2700

uacaaugcua aaagcaagga gaaaaagguc caaaucauua cacugaaggc cucucuuacc     2760

agccaguuca gauccaucuu ugggcuguau aaagugcggg aagugaacga uuaccaucac     2820

ggacaggacg ccuaucucaa uugcgucgug gcuacgacac ugcugaaggu uuacccuaac     2880

cuugccccgg aguucgugua cggcgaguau cccaaauuuc agaccuucaa ggaaaauaaa     2940

gcgacagcua aggccaucau uuacaccaac cugcuccgcu uuuucaccga ggaugaaccu     3000

agauuuacaa aggacgggga gauccugugg agcaauucuu aucucaaaac gauuaagaaa     3060

gagcugaacu accaccaaau gaauaucguc aagaaagugg aaguucagaa gggaggcuuc     3120

uccaaggaga gcauuaaacc caaggggccu uccaacaaac uuauccccgu gaagaauggc     3180

cuggacccgc agaaauaugg aggcuuugau ucuccugucg uggccuacac cguucucuuc     3240

acacaugaaa aggggaagaa accccugauu aagcaggaga uccugggaau caccauuaug     3300

gagaaaacac gguucgaaca aaacccuauc cuuuuucugg aggaaaaggg cuuccucaga     3360

ccccgcgugc ugaugaaacu cccuaaguau acccuguacg aguuucccga agggcggaga     3420

cgccuucugg cuagcgccaa agaggcgcag aagggaaauc agaugguccu cccggaacau     3480

cugcugacgc uucuguacca cgcuaagcag ugucuccugc cuaaccaauc cgagucucuc     3540

gccuaugugg agcagcacca gcccgaauuu caggagauuc uggaacgggu ggucgacuuu     3600

gccgagguuc auacacuugc uaaaagcaag gugcaacaga ucgugaaacu guucgaagcc     3660

aaucagaccg cugaugucaa ggagauugcc gcguccuuua uccaacugau gcaguucaac     3720

gccaugggcg cucccucuac cuuuaaauuc uuucagaagg acaucgagag agcccgcuac     3780

acaagcauua aggaaaucuu cgaugcuacc auuaucuauc aguccccuac agggcucuac     3840

gagacgcgga gaaaaguggu ugacuga                                         3867


<210>  4080
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4080
auguggggcg ugagccuguu cgaggccggg aagaccgcug ccgaaagacg gggauaccgc       60

uccacaagac ggcgccucaa ccacagaaaa uuucggcugc gccuucugga ggacauguuc      120

gaaaaggaga uccucucuaa agaucccagc uuuuucauua gacugaagga agcguuucuu      180

uccccuaaag acgagcagaa gcaauucaag ggcaauaucc uguuuaacga uaaagacuau      240

acggaugcug acuacuauga gcaguacaag accauuuauc aucuccggua cgaucugauc      300

ucucagcacc gccaguucga cauuagagaa gucuaucugg ccauccauca ccucaucaaa      360

uaccgggggc auuuuauuua cgaggaucaa acauucacca cagacggaaa ucagcugcag      420

caccacauca aggccauuau caccaugauu aacagcacac uuaaucgcau cauuaucccg      480

gaaaccaucg auauuaacgu uuuugagaaa auccugcucg acagaaugau gaaucggucc      540

ucuaagguga aguuccugau ugagcuuacg ggcgaagaua aacaggacaa gccccugcuc      600

aaagagcugu uuaaccugau cguggggcuc aaggcuaaac cugccagcau uuucgaacaa      660

gagaaucugg cuaccaucgu cgaaacaaug aacaugucca ccgaacaggu gcagcuugac      720

cugcucacac uggccgaugu ucuugcggac gaggaauaug aucugcuccu gaccgcccag      780

aagaucuaca gcgcuauuau ccuggacgag ucuauggaug gauaugaaua cuucgccgag      840

gcuaaaaagg aauccuaucg caagcaucaa gaggagcucg ugcuggucaa aaagaugcuu      900

aaaagcaaug ccauuacgaa cgacgaaaga gcgaaguuug aguacuucua uacagauuac      960

aucggcgcca aaucuuccaa uuacgaagag agcaagaaca uuaagaaagg ccugucugcu     1020

gccuauggga aguacuccaa agaagagaga cucuuuaagc acaucgagcu gcuucuggcu     1080

aaagaaaaug ugcucaccac aguugagcau gcccugcugg aaaagaacau caccuucgcc     1140

agccuccugc cccuucagag auccucucgc aacgcuguga uuccuuauca gguccacgag     1200

aaggagcugg uggcgauccu cgaaaaucaa gccaccuacu aucccuuucu gcuggagcag     1260

aaagacaaca uucacaaacu ucucacauuc cggaucccuu acuauguggg accgcuggcu     1320

gaucagaagg acagcgaauu ugccuggaug gucagaaagc aggccggcaa aauuacgccc     1380

uucaauuuug aggaaauggu ugauaucgac gcuuccagcg aggccuucau uaagcgcaug     1440

accaacaagu gcacauaccu gauccaugag gacgugaucc cuaaacacuc uuuuuccuac     1500

gcgaaguucg aagugcucaa ugagcugaac aaaauucggc uugaugggaa gcccaucgac     1560

auuccucuga aaaagagaau cuuugaagga cucuuccugg agaaaaccaa ggucacacaa     1620

accagccuga agaaauggcu ugcugaacau gagcacauga cggugucugu ugugcagggc     1680

acacagaagg agaccgaguu cgccaccagc cuccaggccu uucaucgcuu cgucaaaauu     1740

uuugaucggg agacaguguc caaucccgcu aacgaagaga uguucgaaaa gaucaucuau     1800

uggucuaccg uuuuugagga caaaaagauu augagacgca agcugagcga guacccgcaa     1860

cugacagaac agcaacaggu gcagcucgcc cagguccggu ucagagggug gggacgccug     1920

ucccagcggc uuaucaauag aauuaaaacg ccugugagcg gcgaugagga ccacaagcug     1980

ucuaucaacg aaauucucug gcagaccaau gagaacuuua ugcaaaucau ccggaauaaa     2040

gauuaucugu ucaagaaaau uaucgaggaa caguuugaga acgaaacagc ucugcuuaau     2100

aagcagcgca uugacgagcu cgccgcgucc cccgccaaca agaaaggcau cuggcaagcu     2160

auuaagaucg ucaaagaacu ggagaaggug cugcagcagc cugccgagaa caucuucauu     2220

gaauuugcua gaagcgauga ggaaucuaaa cgguccaccc ccagagacaa guucaucgag     2280

aaggccuacg cgcagcucaa aaaggaaaca gauaccuuua aucuggagca ccuuaaagag     2340

cugaagcaac gcagcaaaca gcucuccucu cagcggcugu uccuguauuu cauucagaac     2400

gggaagugua uguacagcgg agaacaucuu gacaucgaga gacucgauuc cuaugaaguu     2460

gaccacauuc ugccccaauc uuacaucaaa gacaauagca uugagaaccu ggcccucgug     2520

aagaaggucg aaaaucagcg caaaaaggau ucccugcuuc ugaacagcuc uaucaucaau     2580

cagaacuacu ccagauggga gcagcucaaa aaugcuggcc ugauugggga gaagaaauuu     2640

cggaaccuga cgcgcaccaa gaucacagac agagauaagg aaggauucau ugcccggcaa     2700

cuuguggaga cccgccagau cacaaaacau guuacccagc ugcuccaaca ggaauauaag     2760

gacacgacaa aaguguuugc uauuaaggcc acccugguca gcggccucag acggaaauuc     2820

gaguuuauca agaaucgcaa cgugaaugau uaccaccacg cccaggacgc uuuccuggug     2880

gcguuucuug ggacaaacau caccucuaau uauccuaaga uugagaugga auaccuguuc     2940

aaaggauauc agcauuaccu caacgagguu ggcaaguccg ccgcuaaacc gaaguuuacc     3000

uucaucgucg aaaaccugag caaacaacag uauaauucua caacgggcga ggugaagugg     3060

aaccccgaag uggauauugc caagcugaaa agaauccuua auuuuaagca gugcaacauu     3120

guccggaaag uggaggagca gagcggggcc cuguucaagg aaaccaucua cccuguugag     3180

gaauccagcu cuaaaacaau cccccucaag aagcaccugg acaccgcuau uuacggaggc     3240

uauacagccg ugaauuacgc guccuaugcu cucauccaau ucaaaaaggg gcgcaaacug     3300

aagagagaga uuaucggcau uccucuugcc guccagaccc ggaucgauaa cagcgaaacg     3360

ucccugcagg ccuacauugc ugagcagauc aaaucugagg uggaaauccu caauggacgg     3420

auucugaagu aucaacugau cagcaacaau gggaaccgcc uuuacauugc cggccccucc     3480

gagcggcaua augcgagaca gcugaucguu ucugacgaag cugccaaagu gauuuggcuc     3540

aucagcacaa agcaggccga ugaggcuaug uuucugaagu auuaccgccu cgaacaccug     3600

gaggccgucu ucgaggaacu uauccggaaa caggcugccg acuaccaaau uuuugagaag     3660

cugaucaaaa agauugaagu gaacaaaguu uauuucuacu ccuguaccua uaaugagaag     3720

gugaagguca ucgaggaacu gcucaaaauu acccaggcga acgccacaaa uggagaucug     3780

aaacuucuga agaugucuaa cagagagggc cgccucggga gcguguccgu ggcucuggac     3840

uuuaagauca ucaaccagag cgucaccgga cucuaccaau cuauugaaga cuauaauaac     3900

uga                                                                   3903


<210>  4081
<211>  3930
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4081
augggcagcg ugggguucgc cggaaucgac aaccaguaca aucuggucag aaccaagggc       60

aaaaacguua uuggggugcg gcucuuugau gaggcugacu ccgccgcgga acgcagagga      120

caccggacaa aucgcagacg gcugcaacgc agacgguggc gccuuagacu gcucgaugac      180

aucuucgcua agccccugca ggccguggau ccuaacuuuc uugcccggcu gaaauauucu      240

uacgucaaua agaaagacca gggccagcaa gaucauuauu acgggggaua uguguucggc      300

agcacggcug ccgaccaggc uuaccaccag gccuauccga ccauuuacca ucuccgcaag      360

agacugaugg aggaugacca gaagcacgau cugcgggaag uuuaccucgc gauccaucac      420

auugugaaau aucgcggcaa cuuucugaau ccccaauccu cucuugacau cgaucagcag      480

uucgacguca cagacuuugc ccaggcucug gccagauucg cugaucacca agcccucagc      540

ugggcgcugg aggccccuau ccgguuucuu gaagcugagc uggccaccgg gcucuccaac      600

ucugcuagag uggacgccgc cauugaggcu uucagcuuug auacaaaggu ugaccgcgcg      660

gccaucaaag aaaugcugaa gggacugucc ggcaaucaga uugauuucac caaacucuuu      720

gugaacgucg acagcgcuga uugggaccag gaggaacgga agcaauggaa gaugaaacug      780

ucugaggagg auuucgacga acaggcccuu cccauccugg agagacucuc ccaggaugaa      840

acagaguucu uucuggccau uaagcgcgcu uacgacggga ucgcccuuau gcgguuccug      900

ggagacgaac agagccucuc uuccgcgaug auuaaggcuu augaggauca uagacgcgac      960

cugaccuuuc ugaaaacgca agugcggacc ccucagaaua gacaggcccu cagcgagggc     1020

uacacaaacu aucugucugu ggaugacaaa aagcacaaac gcggggccaa ggaacuugcu     1080

cagcugaucg aggccuccga ugcuagcgaa caagacaagg ccaccaugcu cgaucggauc     1140

gcgaaugacc aguucgcccc caaacagaga acaaaggcua acggacugau uccuuaccag     1200

cuucaucugg ccgagcucaa aaagauccug gcuaaacaag gccaguauua cccguuucug     1260

cucgauaccu ucgccaagca gggccagucc gucaauaaga uugaggagcu gguucaauuu     1320

cgcgugcccu acuauguggg gccuaugguc cccaaaucug agacggcggg aaacgccgaa     1380

aaccacuggg uggagaagaa ugacggccag acaaaaguua gcgugacccc uuggaacuuc     1440

gaucaggucu uuaaucggga cagagcugcc aaguccuuca ucgaucggcu uacagggacc     1500

gacaccuacc ugauuggaga acccacacuc ccgcgccaca gccugacgua ugagaccuuu     1560

acagugcuga acgaacuuaa uaacaucaga aucgacggca aacggcuccc uguugagacc     1620

aagcaagcua uuguggagga ucuguucaag aaauacagac uggucacaaa gaaacgccuc     1680

caggacuauu uugccucuuu cgggaagcgg gaaguggagc ugaccggacu ugccgaugaa     1740

uccagauuca cgagcucucu gacaagcuac caugaccucc agggccugcu gggcaccgau     1800

uuuaucacca auccccagaa ccacucccuu cucgagaaaa uugucgagau ccaaacagug     1860

uucgaagacu cugauauugc ugagcgcgaa cuggggaagc ugggacucga gcagaagcug     1920

aucccuagac uugcgaaaaa gcauuauacc ggcuggggga aucugagccg gaaacuccug     1980

gacacauccu uuauccacga ucccgaacgc cccgaggagc cuguuagcau uauggaccug     2040

cuuuacacga ccaacaagaa uuucauggaa auccuccaug auucugagua cggaguggaa     2100

gaguggcuga aaucccagaa caugauugac gaucagaagg acauccaaau gagaauugac     2160

gaacugacaa ccagcccggc caauaaacgg ggcaucaagc aggcuuuuaa cguccucgau     2220

gacauuacac aggccauggg ggaggagccc gccuaugugu accuggaguu cgcucgcgaa     2280

aagcaggccu cuagacggac cguuucccgc aaaaagagac uugaaacgcu guauaaaaau     2340

gcggcucuca agaccgaguu uaaagccauc aaggaagccc uggcugagga gagcgaugac     2400

cggaugcaag augaccgccu guaccuuuau uacgcccagc ucggcagaga uauguauaca     2460

ggacagucca ucucuauuga ccaacugagc ucccacuacg auaucgacca cauugugccu     2520

cgggcguuca ucaaggauga cucucuggaa aacaaagucc ucgugaacag aaccgacaau     2580

gcucgcaaga cagauagcgc caccuuuacg gccgacguga aagcuaaggc cuucccccug     2640

uggcagcagc uuaaaaagcu gggccucauu uccgcuaaga aauuucggcu gcugacaaga     2700

accggggauu ucacagagau ggaacgcgag cgguuuaucg ccagacagcu uguugagacc     2760

cgccaaauca uuaagaacgu cgcggcccug aucgaaggac auuucagcca gacccaggcu     2820

guggccauuc gggcugaggu gacaggcgaa cucagacagc ugacgcaaau caaaaaggac     2880

cgcgauauua augacuacca ccaugcccag gaugcccucc uggucgcuac cgcggggaca     2940

uaucuucacc ggcacuuccc uaaaagagac gcccgguuua ucuacaacga guucgauuau     3000

uacacccagc auuggcugaa gaaucaggga gaaaaccgca gacggcaccc cuauucuuuu     3060

gugguuggca caauguccaa ggggaaugag gacuggaccc cggauaaccu caauuaccug     3120

cgcaaaguga ugcaauauaa gacgaugcug augacaagaa aaccugucgg ccccgaggga     3180

gcucuuuaca aggaaacccu gaucgccgcc gacccuaaaa agcggcucgu gggcgcuagc     3240

aaagagagac aggaucccac cauuuacggg ggauauacaa aggaaucuuc cgccuacaug     3300

agccugguuc gcgcgggcgg gaagaaccag cucgugaaaa ucccuguccg gauugcuaau     3360

gagauccauu cuggacagag aaagcuggac gacuaugugc aagccaaagu uaagaaauuc     3420

gaacgcauuc uucugcccaa gaucagccuc ggccagcugg uggaggauga ggggcagcgg     3480

uuuuaccugg ccaccaacga aaugaagcac aaugcuaaac aacuuuggcu ggaccagaag     3540

gucgugacaa cguauaaaag acucaccgcc gaguccccgg uggaagauuu ccugacaguc     3600

uuugacgcgc ucaccagcuc ugcuacaauc caucacuuca aguuuuacca gcgcgaucug     3660

gagcuucugc gggacaacag agccggcuuc caggaucugg ccaaagcuac ccaacucaag     3720

guucugaagg acgugcuuua ugaacugcac gauaaugccg gauggcgcga cccuauuaaa     3780

caguacuuua aggagaucgg ccucaaagug agaaugugga cgaagcugca gaaagaaggg     3840

ggaauuaagc ucaccgauca ggcugaacug aucuaccaau cccccagcgg ccuuuucgag     3900

aagcggcgca gaguccagga ccugcuguga                                      3930


<210>  4082
<211>  3939
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4082
augcagaacg gcuuccuggg gaucaccgug agcuccgagc aagucggaug ggccguuaca       60

aaucccaagu acgaacucga gagagcuucu cggaaagacc uguggggcgu gcgccuuuuu      120

gauaaggccg aaacggcgga ggacagacgg auguuccgca ccaacagacg gcugaaucag      180

cgcaaaaaga accggauuca cuaucucaga gauaucuuuc augaagaggu gaaucagaaa      240

gacccuaacu ucuuucagca acuggaugag agcaauuucu gcgaagacga ucgcacaguc      300

gaguuuaacu ucgacaccaa ucuuuacaag aaccaguuuc cgacagugua ucaccugaga      360

aaguaccuca uggaaaccaa agauaagccc gacauucggc ugguuuaucu ggcuuucucc      420

aaauuuauga agaaucgcgg gcauuuccuc uacaaaggaa accugggcga ggugauggau      480

uuugaaaauu cuaugaaggg guucugugaa agccuugaga aguucaacau cgacuuuccu      540

acacuguccg augaacaggu caaagaggug agagacauuc ucugcgacca caagaucgcc      600

aaaaccguua agaaaaagaa uaucauuacg aucaccaaag ugaagucuaa gacagccaag      660

gcuuggauug gacuguucug uggcugcagc guccccguga aggugcuuuu ucaggauauc      720

gacgaagaga uugucaccga uccugagaaa aucuccuucg aagacgccag cuaugaugac      780

uacauugcua acaucgagaa gggcguuggg aucuacuaug aagccauugu gucugcgaaa      840

augcuguuug auugguccau caugaacgag auucucggag accaucaacu gcugagcgau      900

gccaugaucg cugaauacaa uaagcaccac gacgaucuca agcggcugca gaaaauuauc      960

aagggcacag ggucucgcga gcuuuaucag gacaucuuca uuaacgacgu guccggaaau     1020

uacgucuguu augugggcca ugccaaaacc augagcucug cugaucagaa gcaauuuuac     1080

acguuccuga aaaacagacu caagaauguu aacgggaucu ccagcgagga cgccgaaugg     1140

auugauacag agaucaagaa uggaacccug cuucccaaac agacaaagcg ggacaacucc     1200

gugauuccuc accagcugca acucagagaa uuugagcuga uccuggauaa uaugcaggaa     1260

auguauccgu uccucaaaga gaaccgcgag aagcugcuua aaaucuuuaa uuucgucauu     1320

cccuacuacg ugggcccucu gaagggcguu gugcggaagg gggaaucuac caacuggaug     1380

guccccaaaa aggacggagu gauccauccu uggaauuuug augagauggu cgacaaagaa     1440

gcgagcgccg agugcuucau uuccagaaug accggcaacu guagcuaucu cuucaaugag     1500

aaggugcugc ccaaaaacuc ucuucuguac gaaacauuug agguucucaa cgaacugaau     1560

ccgcugaaga ucaacgggga gccuauuucc guggaacuca aacagcgcau cuaugagcag     1620

cuguuccuua cgggaaagaa ggucaccaaa aagagccuga caaaauaccu cauuaagaau     1680

ggcuaugaug aggacaucga acugucuggg aucgauaacg aguuucacag caaucugaaa     1740

ucccacauug acuucgaaga uuacgacaac cuuucugacg aggaagugga gcggaucauu     1800

cucagaauca ccguuuuuga ggauaagcaa cugcugaagg acuaucucaa ucgcgaguuc     1860

gugaaacuga gcgaggauga acggaagcag auuugcuccc uuagcuacaa aggauggggc     1920

aaccugucug agaugcuccu gaauggcauc acagucaccg acuccaacgg gguggaagug     1980

agcguuaugg auaugcugug gaauacgaac cuuaaucuca ugcagauccu gucuaagaaa     2040

uacggauaua aggcugagau ugagagauac aacaaggaac augagaaaaa uaucuauaac     2100

cgcgaagacc ugauggauua ccucaacauu cccccugccc agcggagaaa ggucaaucaa     2160

cugaucacaa uugugaaauc ccuuaagaaa cgcuguggcg ugcccaacaa gaucuuuuuc     2220

aaggucagcc gggagcacca ggacgauccc aaaagaaccu ccucucggaa ggagcagcug     2280

aaauaucucu acaagagccu gaaauccgaa gacgagaagc aucugaugaa agaacuugau     2340

gagcucaaug ggcacgaacu gucuaacgac aagguguauc uguacuuucu ccagaaggga     2400

cgcugcaucu acagcggcaa aaagcugaau cuuuccagac ugcggaaaag caacuaucaa     2460

aaugauauug acuacaucua uccucucucu gcuguuaacg acagauccau gaauaacaag     2520

gugcugaccg ggauucagga gaaucgcgcc gauaaauaca cauauuuccc ggucgacccc     2580

gagauccaga agaagaugaa aggcuuuugg auggaacugg ugcuucaggg auucaugagc     2640

aaggagaaau acuuucggcu cucuagagaa aacgauuucu ccgagaauga acugguuagc     2700

uucauugagc gcgagaucuc ugacaaccaa cagagcggca gaaugaucgc cuccgugcug     2760

caguauuacu uuccugaaag caagauuguc uucgugaaag agaagcucau cucuuccuuu     2820

aagcgggauu uccaucugau uagcuccuac gggcacaauc accuucaagc ugcgaaagac     2880

gccuauauca ccauuguugu gggaaacguc uaccauacaa aguuuacgau ggaucccgcu     2940

aucuauuuca aaaaccacaa gcgcaaagac uacgaucuga auagacucuu ucuggaaaac     3000

auuucucggg acggccagau cgccugggag agcgggccuu auggauccau ccagaccgug     3060

cgcaaggaau acgcccagaa ucauauugcu gugacaaaga gagucguuga ggugaaaggc     3120

gggcuguuca agcaaaugcc ccuuaaaaag ggccacggag aguauccgcu gaaaaccaac     3180

gauccucggu uuggcaauau cgcccaguac gggggauaca caaacgugac cggcucuuau     3240

uucguccucg uggaaagcau ggagaagggg aagaaacgca uuucccugga auacguuccc     3300

guguaucucc acgagagacu ggaggacgac ccuggacaua aacuucugaa agaauaccuc     3360

gucgaucacc ggaagcugaa ucaucccaaa auccugcuug cgaaggugcg caaaaacucu     3420

cugcucaaga uugacggcuu uuauuacaga cugaaugggc ggagcggcaa cgcucucauc     3480

cugacgaaug ccguugagcu uaucauggau gacuggcaga ccaagacagc caacaaaauu     3540

uccggauaua ugaagagacg cgcuaucgau aaaaaggccc ggguguacca gaaugaguuc     3600

cacauucaag aacuggaaca gcucuacgac uucuaucugg auaaacugga gaacggcguc     3660

uacaagaaua gaaagaacaa ucaggcugag cuuauccaua acgaaaaaga gcaguuuaug     3720

gaacugaaga ccgaggacca augugugcuc cugacagaaa uuaaaaagcu guucguuugc     3780

agcccuaugc aggccgaucu cacccuuauc gggggaucua aacacacggg caugaucgcg     3840

auguccagca acgugacaaa ggccgacuuu ucuauuaucg cugaggaucc ccuggggcug     3900

cgcaauaagg ucauuuauuc ccacauggga gagaaauga                            3939


<210>  4083
<211>  3939
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4083
augcagaacg gcuuccuggg gaucaccgug agcuccgagc aagucggaug ggccguuaca       60

aaucccaagu acgaacucga gagagcuucu cggaaagacc uguggggcgu gcgccuuuuu      120

gauaaggccg aaacggcgga ggacagacgg auguuccgca ccaacagacg gcugaaucag      180

cgcaaaaaga accggauuca cuaucucaga gauaucuuuc augaagaggu gaaucagaaa      240

gacccuaacu ucuuucagca acuggaugag agcaauuucu gcgaagacga ucgcacaguc      300

gaguuuaacu ucgacaccaa ucuuuacaag aaccaguuuc cgacagugua ucaccugaga      360

aaguaccuca uggaaaccaa agauaagccc gacauucggc ugguuuaucu ggcuuucucc      420

aaauuuauga agaaucgcgg gcauuuccuc uacaaaggaa accugggcga ggugauggau      480

uuugaaaauu cuaugaaggg guucugugag agccuugaga aguucaacau cgacuuuccu      540

acacuguccg augaacaggu caaagaggug agagacauuc ucugcgacca caagaucgcc      600

aaaaccguua agaaaaagaa uaucauuacg aucaccaaag ugaagucuaa gacagccaag      660

gcuuggauug gacuguucug uggcugcagc guccccguga aggugcuuuu ucaggauauc      720

gacgaagaga uugucaccga uccugagaaa aucuccuucg aagacgccag cuaugaugac      780

uacauugcua acaucgagaa gggcguuggg aucuacuaug aagccauugu gucugcgaaa      840

augcuguuug auugguccau ccucaacgag auucugggag accaucaacu gcucagcgau      900

gccaugaucg cugaauacaa uaagcaccac gacgaucuga agcggcuuca gaaaauuauc      960

aagggcacag ggucucgcga gcuguaucag gacaucuuca uuaacgacgu guccggaaau     1020

uacgucuguu augugggcca ugccaaaacc augagcucug cugaucagaa gcaauuuuac     1080

acguuccuca aaaacagacu gaagaauguu aacgggaucu ccagcgagga cgccgaaugg     1140

auugauacag agaucaagaa uggaacccuu cugcccaaac agacaaagcg ggacaacucc     1200

gugauuccuc accagcucca acugagagaa uuugagcuga uccucgauaa uaugcaggaa     1260

auguauccgu uccugaaaga gaaccgcgag aaacuucuga aaaucuuuaa uuucgucauu     1320

cccuacuacg ugggcccucu caagggcguu gugcggaagg gggaaucuac caacuggaug     1380

guccccaaaa aggacggagu gauccauccu uggaauuuug augagauggu cgacaaagaa     1440

gcgagcgccg agugcuucau uuccagaaug accggcaacu guagcuaucu guucaaugag     1500

aaggugcuuc ccaaaaacuc ucugcucuac gaaacauuug agguucugaa cgaacugaau     1560

ccgcucaaga ucaacgggga guccauuagc guggaacuga aacagcgcau cuaugagcag     1620

cuuuuccuga cgggaaagaa ggucaccaaa aagucucuca caaaauaccu gauuaagaau     1680

ggcuaugaua aagacaucga gcugagcggg aucgauaacg aauuucacuc caaucuuaag     1740

ucucacauug acuucgagga uuacgacaac cucagcgacg aagaggugga acaaaucauu     1800

cugcggauca ccguuuuuga ggauaagcag cugcucaaag acuaucugaa uagagaguuc     1860

gugaaacuuu ccgaagauga gcgcaaacag auuugcagcc ugucuuacaa gggauggggc     1920

aaccucuccg aaaugcugcu gaauggcauc acagucaccg acagcaacgg gguggaggug     1980

ucuguuaugg auaugcuuug gaauacgaac cucaaucuga ugcagauccu guccaaaaag     2040

uacggauaua aggcugaaau ugagcauuac aacaaagagc acgaaaagac aaucuauaau     2100

cgggaggacc ucauggauua ccugaacauu ccucccgccc aaagacgcaa agucaaccag     2160

cuuaucacca uugucaagag ccugaaaaag accuauggcg ugccuaauaa gaucuuuuuc     2220

aaaguguccc gggaacauca ggacgauccc aagagaacau cuagccgcaa agagcagcuc     2280

aaguaccugu auaaaucccu gaagucugag gacgaaaaac accuuaugaa ggagcucgau     2340

gaacugaacg ggcaugagcu gagcaaugac aagguguacc ucuacuuucu gcaaaaagga     2400

cgguguaucu auuccggcaa gaaacuuaac cugagcagac uccggaaguc uaauuaccag     2460

aacgauauug acuauaucua cccccugucc gcuguuaaug accgcagcau gaacaauaaa     2520

gugcugaccg ggauucagga aaacagagcc gauaaguaua cauacuuccc ugucgacccg     2580

gagauccaga agaaaaugaa gggcuuuugg auggagcuug ugcuccaagg auucaugucu     2640

aaagaaaagu auuuucggcu guccagagag aaugauuuca gcgaaaacga gcugguuucu     2700

uucauugaac gcgagaucag cgacaaucag caguccggcc ggaugaucgc cagcgugcuc     2760

caauacuacu uucccgaguc uaaaauuguc uucgugaagg aaaagcugau cuccagcuuu     2820

aaaagagauu uccaccuuau uuccucuuau gggcacaacc aucugcaggc ugcgaaggac     2880

gccuacauca cgauuguugu gggaaauguc uaucacgcua aauuuaccau ggauccugcc     2940

aucuacuuca agaaccauaa acgcaaggac uaugaucuca acagacuguu ucuggagaau     3000

auuagccggg acggccagau cgccugggaa uccgggcccu acggaucuau ccagacagug     3060

cgcaaggagu augcucaaaa ccacauugcc gugaccaaaa gagucguuga agugaagggc     3120

gggcuuuuca aacagaugcc ucugaagaaa ggccacggag aguacccccu caagacaaau     3180

gauccgcggu uuggcaacau cgcgcaguac gggggauaua ccaaugugac gggcagcuac     3240

uucguccugg uggaguccau ggaaaagggg aaaaagcgca uuucucucga guauguuccu     3300

guguaccugc augaaagacu ugaggacgac cccggacaca aacugcucaa ggaguaucug     3360

gucgaucauc ggaaacugaa ccacccuaag auccuucugg cuaaagugcg caagaauagc     3420

cuccugaaga uugacggcuu uuacuauaga cucaacgggc gguccggcaa ugcccugauc     3480

cuuaccaacg ccguugaacu gaucauggau gacuggcaga caaaaaccgc uaauaagauu     3540

agcggauaca ugaaacgcag agccaucgau aagaaagcuc ggguguacca aaacgaguuc     3600

cauauucagg aacucgagca gcuguaugac uucuaccugg auaaacuuga aaauggcguc     3660

uauaagaaca gaaagaauaa ccaggccgag cugauccaca augagaagga acaauuuaug     3720

gagcucaaga cagaagacca gugcgugcug cucaccgaga uuaagaaacu guucguuugu     3780

ucucccaugc aggcggaucu uacgcugauc gggggaucca agcacacagg caugaucgcc     3840

augagcucua acgugaccaa ggcugacuuu gccgucauug cugaagaucc ucuggggcuc     3900

cgcaacaaag ugaucuacuc ccauauggga gagaaguga                            3939


<210>  4084
<211>  3942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4084
augcacuacc uggacgagau cuucgcuccc caucuccagg aaguggauga gaacuuucug       60

agacggcuua agcaaagcaa uauucacccu gaagacccgg cuaaaaacca ggccuucauc      120

ggcaagcugc ucuuucccga ucugcuuaaa aagaaugagc gcggguaucc uacccugauu      180

aaaaugagag acgaacuccc cgucgagcag cgggcgcauu acccuguuac aaacaucuau      240

aagcugcgcg aggcuaugau uaaugaagau agacaguucg accugcggga gguguaccuc      300

gccgugcacc auaucaugaa guaucgcgga cacuuucuga acaaugccuc cgucgauaaa      360

uucaaggugg gcagaaucga cuuuuacaaa ucuuucaacg uucuuaauga agccuaugag      420

gagcugcaaa acggggaagg gagcuuuacg auugagcccu ccaaggugga aaagaucggc      480

cagcuccugc uugauaccaa gaugcggaag cugaaucgcc agaaagccgu cgcuaagcuc      540

cuggagguga aaguugccga caaggaagag acaaaaagaa acaagcagau ugcgaccgcc      600

augucuaaac uggugcucgg guacaaggcu gauuucgcca cagucgcuau ggccaaugga      660

aacgagugga agaucgaccu gagcuccgaa accucugagg augaaauuga gaaauuucgg      720

gaggaacuua gcgacgcgca aaaugacauc cugacagaga uuaccucccu cuucagccag      780

aucaugcuga acgaaaucgu gccuaacggc augucuauuu ccgagagcau gauggaucgc      840

uacuggacgc acgaaagaca gcuugccgag gugaaggaau aucuggcuac ccagccggcc      900

ucugcucgga aagaguuuga ccaagucuac aauaaguaua ucggccaggc ccccaaagag      960

cgcggguucg aucucgaaaa gggacugaag aaaauucugu ccaagaaaga gaacuggaag     1020

gaaaucgacg agcuccugaa agccggcgau uuccuuccua agcagagaac aagcgcuaau     1080

gggguuauuc cccaucaaau gcaccagcag gagcuggacc ggaucaucga aaagcaggcg     1140

aaauacuauc cuuggcucgc caccgagaac cccgcuacag gagaaagaga ucgccaucaa     1200

gccaaguacg agcuggacca gcuugugucu uuucggauuc cguauuacgu gggcccucug     1260

gucacccccg aagugcagaa agccacgucc ggggcuaagu ucgccugggc gaaaagaaag     1320

gaggauggag agaucacacc uuggaaucuc ugggacaaga uugaucgcgc ugaaagcgcc     1380

gaggccuuua ucaaacggau gaccguuaag gacacauacc ugcugaacga agacgugcuc     1440

cccgcuaauu cccugcuuua ucagaaauac aacguccuga augagcucaa caaugugaga     1500

guuaacggcc gccggcuguc ugugggcauu aagcaagaua ucuauaccga acuguucaaa     1560

aagaaaaaga ccgucaaggu gagcgacguc gccucccuug ugauggcuaa aacaagaggg     1620

guuaauaagc ccagcgugga gggacucucu gauccuaaaa aguuuaacuc caaucuggcc     1680

acguaccugg accucaaaag caucgucggc gauaaggugg acgauaaccg cuaucagacc     1740

gaccuggaga acauuaucga auggcggucu guuuucgagg auggggaaau uuuugcggac     1800

aaacuuacag agguggaaug gcugaccgau cagcagagaa gcgcccucgu caaaaagcgc     1860

uacaaaggau ggggccggcu guccaagaaa cugcuuacag ggaucgugga cgagaaugga     1920

caaagaauua ucgaucucau guggaacacc gaccagaauu ucaugcagau ugugaaccag     1980

ccgguuuuua aggagcaaau cgaccagcug aaucagaagg cuaucacgaa cgauggcaug     2040

acacugcggg aaugggucga gucugugcuc gacggcgccu auaccagccc ccaaaauaaa     2100

aaggcuauuu ggcaaguggu ccgcgugguu gaagauaucg ugaaagccgu cgggaacgcg     2160

ccuaagucca uuagcaucga guucgccaga aaugagggaa acaaaggcga aauuacccgg     2220

ucuagacgca cacagcugca gaaacuuuuu gaggaccagg cucacgaacu ggugaaggau     2280

accucccuca cagaggaacu ggagaaggcc cccgaccuga gcgaucggua cuaccuuuau     2340

uucacgcagg gaggaaagga cauguacacc ggcgauccua ucaauuuuga cgagaucucu     2400

acaaaauaug auauugacca cauccucccc caguccuucg uuaaggauga cagccuggac     2460

aacagagugc uggucucccg cgcugaaaau aacaaaaagu cugauagagu gccggccaaa     2520

cucuacgccg cuaagaugaa gccuuauugg aaucagcugc uuaaacaagg gcugauuacc     2580

cagcggaagu ucgagaaccu cacaauggac guugaucaga acaucaaaua ccgcagccug     2640

ggcuuuguga agagacagcu ggucgaaacc cggcaaguga uuaaacuuac ggcgaauauc     2700

cucggaucca uguaucagga ggccggcacc gacaucauug aaacacgcgc ugggcugacc     2760

aagcagcuga gagaggaguu cgaucucccc aaggugcggg aagucaacga cuaccaucac     2820

gccguugaug ccuaccugac aaccuuugcu ggacaauauc uuaaucgcag auacccuaaa     2880

cugcggucuu ucuuugugua uggcgaguac augaaguuca aacaugggag cgaccucaag     2940

cugcgcaacu uuaauuucuu ucacgaacug auggagggag auaaauccca gggcaaggug     3000

gucgaccagc agacggggga acuuaucaca accagagaug agguggccaa gagcuucgac     3060

cggcugcuca acaugaaaua uaugcugguu ucuaaggagg ugcaugacag auccgaucaa     3120

cucuacggcg cgacaauugu caccgcuaaa gaaagcggaa agcugaccuc ucccaucgag     3180

auuaaaaaga agcgcccugu ggaccuuuau ggcgccuaca caaaugggac guccgccuuu     3240

augaccauca ucaaauucac aggaaacaag cccaaauaca agguuauugg cgugccgacc     3300

acaagcgcug ucgggcugaa acggguggga aagccuggcu cugaaagcua uaaucaggag     3360

cuccacagaa ucauuaaguc caaccccaaa guuaagaaag auuuugagau cguggucccc     3420

cacgugagcu acucucagcu gauuguggac gaugacugca aguucacccu ggccuccgau     3480

acguaucagc auccugcgac acaacuuguc cugagcaaag aauccaugga gaucauugcu     3540

gacgauuuca aguuuguuaa agaaaauacc gccaccgccg acgagcagcu cgugcgcgug     3600

uucgaugaaa ucgucaacca gaugaaucac uacuuuacaa ucuucgacca gcggucuaac     3660

agacaaaagg ugaccaaggc ucgcgauaaa uuuguuagcc ugccgacaga guccgaguau     3720

gaaggggcca agaaaaccca ggugggcaag acggagguca uuacaaaucu ccugauggga     3780

cuucaugcua acucugccca gggcgaccug aaagugcucg ggcugagcac cuucggauuu     3840

uuccaguccu cuggcgggcu gaaccuuagc gaagacgcga ugaucguuua ccaauccccc     3900

acaggacugu uugagcggag aauuugucuc aaggauaucu ga                        3942


<210>  4085
<211>  3942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4085
augagcuuca aggagaacuc caaaaucuac uauggccugg acauugggac cgauucugug       60

ggauggagca caacgaauga aaaguacgag cuccugagau auaaaaacaa ucuuaugugg      120

ggcgucuccc uguuugaagc cgcuucucuc gccgaggaac ggcgcgggca cagaacccag      180

cggcgcagau acgaccggcg ccaacagaga guugcgcugc uuggagagcu guucgcuaag      240

gagauccuca aaacagaucc cgacuuuuuc cugcggcuga aggaaagcuc ccucuauccu      300

gaggaucgca ccucuaagaa cgugaauaca uacuuugacg augccgacuu caaagauagc      360

gacuauuuua agauguaccc gaccgugcau caccugauua aagaacuuuc cgagagcaac      420

aagccccaug augucagacu gguguaccuc gccugcgcuu ucaucguugc ccaccggggc      480

cauuuucuga auggggcuga cgagaacaau gugcaggccg uccuugauuu cgacucuucc      540

uauugugaau uuacagacug guucaaaagc aacgauauug aggacaaucc uuuuucugaa      600

uccgugaaga acgaguucag cguuauccug cgcaagaaaa ucggaauuac cgcgaaggaa      660

agagagauca aaaaucuccu guucggcacg accaagacac ccgauugcua caaagacgag      720

gaauauccua uugauaucga cgugcugauu aaguuuaucu cuggcgggaa aaccaaccuc      780

gccaagcugu uccggaaucc cuccuacgau gagcuugaca uucagacagu cgaaguggga      840

aaggcugauu uugccgacac caucgaucug cucgcuagcu ccauggagga cacggacgug      900

ccucugcuuu cugccgucaa agcgauguau gauuggagcc ugcucaucga cguucugaag      960

ggccaaaaga caauuuccga ugccaaggug ugugaauacg agcagcacaa aagcgaccug     1020

aaggcucuca aguauaucgu gcgcaaauac cuggauagag cccaguacga cgagauuuuc     1080

cggaccgcug gggaaaagcc gaacuauguc ucuuacuccu auaacgugac agauguuaaa     1140

cuuaagcagc ugcccagcaa uuuuaaaaag aagaacucug aggaguucug caaauacauc     1200

aagagcaaac ucgaaaagau uaaaccugaa uccgacgaug aggccgugua uaaugagcug     1260

aucgaaaagu guaacucuaa gacccuuugc cccaaacaag ucaccgacga gaauagagug     1320

aucccuuacc agcuguauua ccacgaacuc agcaugauuc uggauaaggc cuccgcuuac     1380

cuggacuuuc ucaacaaaac agaggaugga aucagcguua agcagaaaau ucugacgcuu     1440

augaaguucc gcauccccua uuuugugggc ccgcugguca aacggaauga gaccgacaac     1500

guguggauug ucagaaaggc ggaagggcgc aucuacccuu ggaauuucga gaacauggug     1560

gacuuugaua agucugaaga cggauucauc cggagaauga cauguaaaug caccuaucuc     1620

gccggcgagg auguucugcc caaguacucc cugcuuuaua gcaaauacac agugcucaau     1680

gaaauuaaca auaucaaggu caacggcgag aaaauuucuc cugagcugaa gcaggacauc     1740

uuuaaugaac uguucaugaa gaccucccgc gugacgguua aaaagauuac agagcuccug     1800

aaacggaagg gggcuuucag cgaagagaaa ggagauuccc uuucuggcgu ggacaucaac     1860

auuaagagcu cccugaaguc uuaucucgau uuuagacgcc ugcuggaaaa cgggagccuu     1920

uccgagaaug acgucgagcg gaucaucgaa agaauuaccg ugaccacaga uaaaccccgc     1980

cucgugagcu ggcugaaggc cgaguacucu gaccugcccg ccgaagauau ccgguacauu     2040

uccagacuca gcuauaaaga cuacggacgg cugucugcua aacuucugac cggcuguuau     2100

gagcucgaua caaacacggg ggaaaucgga ggccgcucca uuaucgacuu caugugggcc     2160

gagaauauca accugaugca aauucugagc gacucuuacg gcuauaagag cuuuaucgag     2220

gaagagaaua agaaguacua uaugauuaac ccuaccgggu ccaucgcgca gacacuuaga     2280

gaaauguacg uuagcccguc ugucucccgg gcuauuauca gaaccaugga gaucgugaaa     2340

gagcuccgca agauuaccaa aaaggauccc gacaaaaucu ucguggaaau ggcccgggga     2400

ggcaagccug aggaaaaagg gaagagaaca agcucccgca gagagcagau ugaaaagcug     2460

uacgauucug ccaaggcuuu ugucagcgac gaggauaucu cccauaugcg gucucaacug     2520

ggaagccucu ccgacgagca gcugcgcaga gaaaaguauu accuuuauuu cauucaguuu     2580

ggcaaaugca uguacucugg ggaggccauc gauuucagcc ggcugggcga caaucacugu     2640

uaugauaucg accauauuuu uccccagucc aagaucaacg augacagccu ccacaauaaa     2700

gugcugguua agucucaacu gaacggagaa aaguccgacg auuacccuau uaaagcugag     2760

auccgcaaua agaugcaccc ccuuuggaaa aaccucuucu auagagaccc gaagaauccu     2820

gccgauaaag ugaaguacga acggcugacg cgcagcaccc ccuuuacaga ggacgagcug     2880

gcgggcuuca uugaaagaca gcucgucgag acccggcagu cuacaaaggc cguggcuacc     2940

cugcuuaaag aaauguuucc ugauuccaag aucguuuacg ugaaagccgg gcaggucagc     3000

gaguuccgcc augacuucga uaugcugaag ugcagagaaa uuaacgaccu ccaccaugcu     3060

aaagaugccu aucugaauau cgugaucgga aacguucacg acgugaaguu uacgucuaac     3120

cccaugaauu ucgucaagaa cgcggauaaa cauuacacaa uuaaggugaa agagacccug     3180

aagcacaaag ugucccggaa uggcgagaca gccuggaacc cugaaaccga cuuugauacc     3240

gucaagagaa ugaugagcaa aaauuccguu cgcuaugugc gguacugcua uaaaagaaaa     3300

gggggauucu uuaaccaaaa ucccgaccgc gcuggcaacc cggaccuugc ccggcugaaa     3360

aaggaucucg acccugugaa auacgggggc uauaauagca agucuaucag cuguuucucc     3420

cugauuaaau gcacaggagu cggcgcugug aucauucccg uugagcuccu gugugaaaag     3480

agauacuuuu cugaugacag cuucgccucc gaguacgccu auucugugcu uaagaacgcu     3540

cugcccgcga aaaauaucgc caagaucagc auugaugaca ucuccuuucc ucucaaacgc     3600

cggccgauua agaucaacac gcuguucgag uuugaugggu acagagucaa uauucgcucu     3660

aaagacagcu aucacuacuu caccaucucc agcgcuaugg ccgccaucua uucuaaggau     3720

acauccgacu acauuaaggc ugugagcucu uauaucgaua aauccgacaa gggaagcaaa     3780

uucaagcccg gcgaagccuu ugauguucug uccaaccuua aaguguacga cgagauugcg     3840

aagaagugca ucucugaacc uuucagcaaa auuuccaagc uggcugaggc cgggaaacgg     3900

uggaagaaag aagagaucag ccuccagaau ugucugcucu ga                        3942


<210>  4086
<211>  3945
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4086
augcugaaga aagacuacag caucggccuc gauauuggga ccaacuccgu gggacacgcc       60

gucguuacag acgauuauaa ggugcccacg aaaaagauga aaguguucgg cgacaccucu      120

aagaagacaa ucaaaaagaa uaugcugggg guccuucugu uuaacgaggg acagaccgcu      180

gccgauacaa gacucaaacg gggcgcgcgc agacgguaca cccgcagaaa gaaucggcug      240

cgcuaucuuc aagaaauuuu cgcuccugcc cuggccaaag uggacccgaa cuuuuucuac      300

agacucgagg aaagcucccu gguugcugag gauaagaagu augacgugua ccccaucuuu      360

gggaaacggg aagaggagcu gcucuaucau gauacacaca agaccauuua ccaucugcgc      420

ucugaacuug ccaauaacga cagaccugcu gaucugcggc ucgucuaccu ggcccuugcg      480

cacaucauca aauaucgcgg aaauuuccug cucgagggcg aaauugaccu gagaacgacc      540

gauaucaaca agguguuugc cgaguucagc gagacacuga augaaaacuc cgacgagaau      600

cucggcaaac uggacguugc ugauauuuuu aaggacaaca ccuucucuaa aacaaagaaa      660

agcgaggagc uucugaaacu cuccggggcc aagaaaaauc agcuggcuca ucagcuuuuu      720

aagaugaugg ugggaaacau gggcagcuuc aaaaaggucc uggggaccga ugaggagcac      780

aagcucucuu uuggaaaaga cacguacgag gaugaccuga augaucugcu cgccgaagcg      840

ggcgaccagu aucuggauau cuucguggcc gcuaagaaag uguacgacgc cgcuauucuu      900

gccuccaucc uggaugucaa ggacacacaa accaaaacag uuuucagcca ggccaugauu      960

gagcgguaug aggaacacca gaaggaccuc aucgaacuga agcgcguguu uaaaaaguac     1020

cuucccgaga aaugccauga uuucuuuucu gagccuaaga ucuccgggua ugcuggauac     1080

auugacggca aagugagcga agaggauuuc uacaaguaua ccaagaaaac ccugaagggc     1140

auccccgaaa cagaggaaau ucuccagaaa aucgacgcga acaacuaccu gagaaagcaa     1200

cggacguuug auaauggggc cauuccucac cagguccauc ugaaagagcu cguggcuauc     1260

guugagaacc agggaaagua uuacccguuc cugagagaaa auaaagacaa guuugagaag     1320

auccuuaacu uccgcauucc cuauuacgug ggcccucugg cccggggaaa uucuaaauuu     1380

gccuggcuca ccagagcugg ggaaggcaag aucacacccu auaacuucga ugagaugauu     1440

gacaaagaga ccuccgccga agauuuuauc aagcgcauga caauuaauga ccuguaccug     1500

ccuaccgagc ccguccuucc gaaacacagc cuccuguacg aacgguauac gaucuucaac     1560

gagcuggcgg gggugagaua cguuacagaa aauggagagg cuaaguauuu ugaugcccag     1620

accaagcgcu ccaucuucga acucuucaaa cuggaccgga aggugucuga aaagaugguc     1680

auuaagcacc uuaaaguggu caugccugcc auccgcauuc aagcucugaa gggccucgau     1740

aacggcaagu uuaaugccag cuacgggacc uauaaagacc ugguggacau gggaguugcu     1800

cccgagcugc uuaacgauga agucaauucc gagaaguggg aagacaucau uaaaacacuc     1860

accaucuucg agggcagaaa gcugauuaaa cggagacugg agaacuaccg cgauuuucuc     1920

ggggaagaca uccugcggaa acuuagcaga aagaaauaua caggaugggg ccggcugucu     1980

gccaagcucc uggaugggau cuacgacaaa aagacgcaua aaaccauucu ggauugucuu     2040

augacagagg acuacuccca gaauuucaug cagcucauca acgaugacac cuauagcuuu     2100

aaggaaacaa uuaaaaacgc gcaagugauc gagaaggaag agacccuggc caagacggug     2160

caggagcugc cuggcucucc cgcuauuaaa aagggaaucc uccagagccu ggaaaucguu     2220

gaugagauua ucaaagugau gggcuacaag cccaaaucca uugucgugga aauggcccgc     2280

gagacccaga agacacacgg gaccagaaag cgggaggaca gagugcaaca gaucguuaaa     2340

aaucuuaagg augcuaacga acugccuaaa aagcucccgu cuaaugccga gcugagcgac     2400

gaacgcaaau aucuguacug ccuucagaac ggacgggaca uguauacagg cgcgccccuc     2460

gauuacgacc aucugcaguu cuaugauguc gaccacauua ucccucaauc cuuucugaag     2520

gaugacagca ucgagaauaa ggugcucacc auuaaaaagg aaaacgugag aaaaacgaau     2580

gggcugcccu cugaggccgu cauccagaag augggauccu ucuggaaaaa gcugcuugau     2640

gcuggcgcca ugacaaacaa gaaauacgac aaucuccgca gaaaccugca ugggggccug     2700

aaugagaaac uuaaggaacg guuuauugag cgccagcugg uggaaaccag acagaucaca     2760

aaguacguug cucaacuccu ggaucagcgg cucaacuaug acggaaaugg cguggagcug     2820

gaugaaaaga uugccaucgu cacccuuaag gcccagcugg cuagccaguu ccgcucugag     2880

uuuaagcuca gaaaagugcg ggcgcugaac aaucugcacc acgcccauga cgcuuaccuu     2940

aacgccguug uggccaaccu gaucauggcu aaguauccug agcucgaacc cgaguucguc     3000

uacgggaaau aucgcaagac caaauuuaag ggacugggca aagccacagc gaagaauacg     3060

cucuacgcua acgugcugua uuuccuuaag gaaaaugagg uuuacccguu cugggacaaa     3120

gccagagauc ugccuaccau uaagcgguac cucuaucgcg cccaagugaa caaagucaga     3180

aaggcugaac ggcagacagg gggauuuucc gacgagaugc uggugcccaa aagcgauucc     3240

ggcaagcugc uuccuagaaa ggaggggcug gaccccguga aauacggcgg auaugccaag     3300

gcggucgaau cuuacgcugu ucucaucacc gccgaugagg ugaaaaaggg caaaacaaag     3360

aaggugaaaa cccuggucaa uauuccuauc auugacagca agaaauauga agccgauccc     3420

acggcuuacc ucgccucccg cggguauaca aacgugacca auucuuucau ccugccgaag     3480

uacagccuuc uggaggaccc ugagggacgg agacgcuacc uggcuuccuu uaaagaguuc     3540

cagaaggcca acgaacucau ucugccccaa caccuuguug aacugcucua uugggugaau     3600

gcgaaggaug gcgagcagaa acuggaagac cauaaggccg aguuuaaaga acucuucgau     3660

aagaucaugg aauuugcuga caaauacguc guggccccca agaacagcga gaaaauccgg     3720

agacuguaug aagagaauca ggaugcuacc ccuauggaac uugggaagaa cuucguugag     3780

cugcugcgcu acacagccga cggagccgcu ucugacuuua aguucuuugg cgagaauauu     3840

ccgcggaaaa gauauaacuc cgcggggagc cuccugaaug gcacccuuau cuaccagucu     3900

aagacaggac uguaugaaac gcgcauugau cucggcaaac uguga                     3945


<210>  4087
<211>  3948
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4087
augggcuaca gcguggggcu ggacaucgga accggcuccg ucgggugggu ugugacaaac       60

gaggauggaa agcucgccag agcuaaaggc aagaaucuga uuggggugcg gcuuuucaaa      120

acggccgaac ccgcggcuga ccgcagaacc aaccggacaa cccgcagacg gcugucucgc      180

agaaaguggc ggcuccgccu gcuugaugag cuguuugcca augaacucgg aaaaaucgac      240

agcaacuucc ugacaagacu gaaguauucc uacguccacc cuaaggauga cgccaaucgg      300

gcuaacuauu acggcggcua ucucuuuccg acccaggagg aaacaaagaa guuccaugcc      360

aaauacccca ccauuuauca ucugcgccuu gcucugauga cggagucuag acaauuugau      420

cuccgggaga ucuaccuggc caugcaccag auugugaagu accgcgggca cuuccuuaau      480

agccagucca aaaugaccau cggaaacaca uauaauccuc aggaccugca aaccgcgaug      540

gaaaacuacg ccgaggcuaa gggccucucu aucgaacuga agcagcccga ggagaugguu      600

gccauucuga caggggaagc uggauuuggc cucagcaaaa aggccaaagc ggagaagcug      660

cuuaauaacu ucuuuuucga uaccaaagaa gagaaggcca cggugcaggc uauccuggcc      720

gggauugucg gaaauacaau cgacuuuacc aaaguguuca acagacagcu cacaggcgau      780

acccugaagg cuuggaaacu uaaacuggac uccgaaaccu uugauguuga cagcgagccu      840

auucucucug accuggauga cgaugagaca gaacuguucu ucgccauccg gcaagccuuu      900

gacggcuuca cgcucaugaa ucugcuuggg gaucagaccu ccauuagcgc ugcgauggug      960

gagagauaua cacagcauca cgacgaucug cucaccguca aacagguggc caagaagcaa     1020

ggacugucuc acaaagaauu uuccaacauc uacagcgcuu uccuuaagga cgauacagac     1080

aagggcauga aaaagcugcu cgaggaaucu aaccugucca ccgaggaugu gaaugggauc     1140

cagcugcgca uggacagcca ugacuuucuc ccccagcaac ggacgaaagc caacuccguc     1200

auuccucacc agcugcaucu ugccgagcug gaagcuauca uugagaauca gggacaguau     1260

uacccguucc uccuggaaac auuugagaag gaaggcaccg ccgcgaacaa aaucgcugag     1320

cugcuuaagu uccgcauucc cuauuacguu gggccuaugg ugaccaagaa agauguggag     1380

aaugccggag gcgacgccac aaaccacugg gucaccagaa augaaggcua cgagaagucu     1440

gcugugacag ccuggaacuu ugaucaaguu uucaaucggg accaggcugc ccucgauuuu     1500

auccagagac ugacggggac cgacacauau cugaucggag aacccacccu cccucagaac     1560

agccugaaau accaaauguu cacagugcuu aaugagcuga acaaugucaa ggugaacggc     1620

cgccggauug auaaucagac caaacacgac cucauccagg aucuguuuaa guccaagaaa     1680

acgguuagcc ugaaggcguu ccaggacuau uaccaaucuc aggggaaauc cgauauucag     1740

guggucggac uugccgacaa gaccaaauuc aacagcaacc ucucuacaua uaucgaucug     1800

agcaagaccu uuuccgcuga ggaaauggag aauccccaga accaagaccu gcucgaacag     1860

auuguggaga uccagacagu cuucgaagac ucuaaaaucg ccgagagaga gcugagcaaa     1920

cuugaacuga ccccggagca agugacgaag cucgcuaaga cacauuacac cggcuggggg     1980

aaucugucca acaagcugcu uacaaccccu auuguugaug ugagcggcaa aaaggucucu     2040

auccucgacc agcuggccaa aaccuccaag aauuuuauga gcauuaucuc uaacgaacag     2100

uauggagugc aggauuggau uaaggagcaa aauacaacgg aaaccacagg cacccuggag     2160

gacaaacucg cggaucugac aaccuccccc gccaacaagc gcgggaucaa acaggcuuuc     2220

aauguucuuu uugacauuca gaaggccaug aaaagcgagc cugaucgggu guaccuggag     2280

uucgcuagag aaacgcagac auccguccgc accaugucuc gguacaacua ucucaaggga     2340

cuguaccaaa gcaagucccu ggacgccgaa gccaaaaauc uuaagaaaga gcucgcugaa     2400

accgaucaga agaaauuuga caacgauaga cuguaucugu acuuccagca gcaaggcaag     2460

gacauguaua cagggcagcc caucaauauc gauaagcucu cuagcgacua cgacauugau     2520

cacaucauuc cccaggcguu uaccaaagac gauuccaucg acaaccgggu gcuggugagc     2580

cgcccugaga augccagaaa gucugauucc ggaacauaua cgaccgaggu ucaagcugcc     2640

gccagcggcc uuuggaaaac acugguccag ucuggguuca uuucccggaa gaaauacgac     2700

agacucaccc augcuggcga uuacagcgaa ggacagaaga caggcuuuau cgcccgccag     2760

cugguggaga cccggcaaau cauuaaaaac guggcggcuc ugaucgaauc ugaguucgac     2820

cagacgcagg ccgucgccau uagagcugag aucaccgggg auaugcgcag acuuguggcc     2880

auuaagaagc accgggaaau caacagcuuu caucacgcgu ucgacgcucu gcucaucaca     2940

gccgccggac aguauaugca aaaacgcuac ccggauagag acuccaauaa cguuuauaau     3000

gaguucgacc gguacaccaa cgauuaucug aagaaacucc gcgaaucuag cuccaaggac     3060

gaggugagaa aacugaagcc cuuuggcuuc auugucggga caauggcuaa gggaaaugaa     3120

gauuggacca cagagcagac cgaguaccuu cggcacauca ugaacuuuaa aaauauucug     3180

acgacauaua agaccggccc ugaccgcggg ccccucagca aagaaaccau cuacgccgug     3240

gauccuaagg cuaaacugau uggcacaaac aagcauagac aggacguuuc ccuguacgga     3300

ggcuauaccu accccuauuc ugccuacaug acgcuugugc gggcgaaugg gaagaaccag     3360

cuggucaaag ugagccgcuc cgccgcuucu cucaugaaaa gcggacaacu gaagcucgag     3420

gauuauguuc agcagcagcc ggaagugaag aaauucgaga agauccugcu ugaacaucug     3480

ccucucggcc aacuggucga caaugauggg aagcugaugu accuuacauc cuaugaguuu     3540

uaccacaacg ccaaacagcu guggcucucu accgagaaug cugaccugau cagcucccuc     3600

agcauugauu cugacgauca gaagcugauc aaagccuucg acauucuuac agccgacucc     3660

gugaucgaaa gauuucccuu cuacgagauu gaucugaaga aacugaucga acuccgggac     3720

aaguuugugc ugauugauaa caaacuugac guccugagag uugugcucaa ggcucugcaa     3780

cucgauagcg cgcagcagaa gccucugaaa cuuaucgaca aggccgcuac cgauuggaaa     3840

aauuaucgcc aggccggagg caucaagcug ucugacaacg ccgagauuau cuaccaaucc     3900

acaaccggga uuuucgaaaa acggaucaag auuagcgauc ugcucuga                  3948


<210>  4088
<211>  3951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4088
augcugcaga agaacuacac caucggccuc gacauuggga caaauagcgu gggauaugcc       60

gucaucaacg auaauuacaa aguucccgcu aagaaaauga aggugcuggg caacacggac      120

aaaaagacca uuaagaaaaa ucuuaugggg gugcuccugu ucgaugccgg agagacagcg      180

gcuaccacaa gacugaagcg gggcacccgc agacgguaua cacgcagacg gaaccgccuu      240

agauaccugc aagaaauccu cgggccugag cuggccaaag ucgacgccaa uuuucugcac      300

cggcucgcug aauccucucu ggugaccgag gauaaggagu ucagcgccua uccgauuuuu      360

ggagaccgcg aggaggaacu ucuguaccau aaaacguaua agaccaucua ccaccucaga      420

uccgcucugg ccaacaagga ugagaaagcg gaccuucggc ugaucuaccu cgcccuggcu      480

cauauuauca aguaucgcgg caauuuccug auugaaggcg agaucgagcu cucugauaca      540

gacauugaua aaguuuacgc cgacuuuugc cggagcuuca acgaacuguu ugauaccgag      600

gugcuucagg aaaucgaggu cgaagcuauu uucaaggaca aaacacuguc caagaccaaa      660

aaggccgagg agcucgugaa gguuucuggg gugaaaaaga aucaggucgc gcaccaguuu      720

cugaaaauga uggugggaaa cacggugaau uucaagaaag ucuuuaaccu uacagaagac      780

caaaagcuga gcuucggcac cgauuccuau gaggacgaac ucaaugaucu gcuggccaag      840

agcgacgaua gauaccucga cguuuuugug gcugccaaaa agguguauga uacagcuauc      900

cuggccaaca uccuuaacac caaaaauucu gucgccacca agacaauuuu cucccagagc      960

augaucgagc gcuucaaaga ccaucagaag gaucugaagg aacucaaaca gcuguuuaag     1020

acguaccuuc ccgagaaaua uccugacuuc uuugggaacu cuaaggagga uggauacgcu     1080

ggcuacauug aagggaaaac caaugaggaa aaguucuaua cacacaccaa gaaaauccug     1140

aagaacauuc cccaagcggc cuccgugcuc gcucagaucg acguuggaaa uuaccugaga     1200

aaacagcgga cauuugacaa cggcgugauc ccucaccaga uucaacugaa ggagcuccug     1260

gccaucauug aaaaucaggg caaauauuac cccuuccuuc uggagaacaa ggagaaaauc     1320

gccaagauuc ucaccuuuag aaucccuuau uacgucgggc cgcuggcucg cggaaauagc     1380

cgguucgccu ggcugaagag agugggcaaa gaaaagauca cgcccuuuaa cuucgagaau     1440

guuguggauc uugaaucuuc cgcggagcgc uuuauugagc ggaugacacu caacgaccug     1500

uaucugccug aagagaaagu ccuccccaag cauagcaugc uguacgaaaa auucuccauc     1560

uuuaaugagc uugcugggau uagauacauc accgauaacg gagaaaugaa guuccuggac     1620

gcccagacca agcaaucuau uuucgagcuc uuuaaacagg agcgcacagu gaaggaaaag     1680

aagguccugg aucugcuuaa aacccucaug ccuagcgagc gggugguugc caucaagggc     1740

auugacaaug ggaaguucaa cgcuuccuuu ggaacauauc acgaucugag caaaaugggc     1800

uucaagacgg agaaaaucgc cucugaaucc aaccaggcua gaugggaaga gaucauuaag     1860

acccugacaa ucuuugagga ccgcaaacuc auuaagagcc ggcugagaaa guacgaugac     1920

uuccuuaaug aaaaagagau cggcgcccug ucuaagcgca aauauaccgg guggggacgg     1980

cucagcgcga agcugauuga uggcaucuac gacaaaaaga cacaucagac cauccuggau     2040

uuucuuauga acgaagacua uaaucaaaac uucaugcagc ucauuuccaa ugacaacuac     2100

ucuuuuaaaa agaucauuga ggauagccag gugaucaagg aagagggguc ccugacggag     2160

gucauucagg aacugcccgg aagcccggcc aucaaaaagg gcauccucca aucuauugag     2220

aucguggacg aacugguuaa agugaugggc gggaauccua agaaaauugu cguggagaug     2280

gcuagagaga cccagaagac acacggaacc acacggcgcg aagccagaau caaggagauu     2340

aucaaaaacc uuaauaagcu gaaacucaac gaacugccca aggaucugcc uuccaaugag     2400

gaacuuagcg acgagaaagu guaucucuac ugucugcaga acggccggga uauguacacc     2460

ggggcucccc uggaccucga uaaucugucu caguaugagg uugaccacau cauuccccaa     2520

uccuuccuua aggaugacag caucgaaaac aagguccuga cggccaaaau ugaaaauucc     2580

agaaagacaa acggacuccc uucuguggaa aucgcgaaaa agaugggcgc cuuuuggcgc     2640

agccugcuga aagugggggc uauuuccgag aagaaguacc ggaaccuuag acgcucucug     2700

cauggaggca ugaccgaaaa acucaaggag acauucguca aaagacagcu gguggagacc     2760

cggcagauca ccaaguaugu ugcccagauu cucaauagcc gcuuuaacga agagaaaaau     2820

gaagauaagg uggcuaucgu cacacugaag gcccaacuua cgucccaguu cagaaaagac     2880

uucgagcugu acaaggugcg ggaaaucaac aaucuccacc augcccacga ugcuuaucug     2940

aacgcgguuc uggccacccu ucugaugacc agcuuuccgg agcucgagcc cgaguucgug     3000

uacgggaaau aucgcaagac aaaauuuaag ggccugggaa aagcuacagc caagaauaac     3060

cucuacacca auauucugca gcuucugaag gaagaaucug cccaauacug ggacacgaaa     3120

gagagagacc ucgcuacaau caagaaaaac gucuaucagg cccaggugaa uaagguucgg     3180

aaaguggagc gccagaccgg cuccuucuac gaugaaaccg ucgcgaaaaa gagcgcuucu     3240

caaaaacugu uuccuagaaa gaacgggcug gaccccguga aauauggagg cuaccagcgg     3300

gccaaugagg gguauucccu ucuguuccgc gccgauaugc ucaacaagag aggaaaaaca     3360

aagagcguga agacccuggu caaaauuccu aucauugaau cugcucagua ccucgccgac     3420

cccacagagu aucuggcgaa gcggggcuac uacaauguua gcgaacauuc ccuucugcug     3480

ccgaaauuua gccuccugga gaacaacaau gggcuuagac gcuaucuggc uucuuccaac     3540

gaguuccaga aggccaauga acucauccug ccuaaacacc ucgugcggcu gcuuuacugg     3600

agccaaucca agcagagaaa ggaggaacug aacaaacacc gcgccgaguu ugcugaacug     3660

uucacguaua ucauggauuu ugccgacaag uacguggucg cucccaaaaa uucugagaag     3720

auuaaaaaca ucuaugagaa uaacaaggau agcuccgugg aaguucucgg caaaaauuuc     3780

auugagcugc uuaaguacac cgccgacgga gcggccucug auuucacauu uuucgacacc     3840

aagaucccuc ggaaaagaua uaacagcgcu ggcgaucugc ucgacgggac acugauuuac     3900

caguccaaga ccggacucua cgaaacgcgc aucgaccugg gcgccaucug a              3951


<210>  4089
<211>  3957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4089
augaugauca agcugaacaa uaugaaaaag auucucggcc uggaccuugg gaccaacagc       60

aucggauggg ccguggucga gacagaagau aauaaaaagu ucgagcugcu cgacaaaggc      120

guuagaauuu uucaggaagg ggugaagauc gagaagggaa uugaauccuc uaaagcugcc      180

gagcggacgg gcuaccgcag cgcgagacgg aucaaguucc gcagacggcu gcgcaaaauc      240

gagacccuua aggugcugau ugaaaacaau augugccccc ucuccaugga ugagcugauc      300

gucuggaaag aaaacaagaa uaacuauccu aagaauaaaa ucuuuuacaa cuggcugaag      360

acaaaagacc cgaaggaggg gaccaaugga acauauauua accccuacua uuucagaacc      420

gaggcuauua aacggaagcu cgaaaauaca aacgaugugg gccgcgcccu guaccaccuu      480

gcccaaagac ggggcuuuaa aucuaaucgc cuggagagca ccgacgaaaa guccagaggg      540

aucguuaaga cggagauuaa caaacucucu agcgaacuga acggacggua ucuuggcgag      600

uacuucuacg agcuguaucu caagggggaa aagauccgca agagacauau cucccgggag      660

gaacacuacc ugaaugaguu uaaaaagauu ugugaagugc agaacuucaa uaaggagcug      720

gcugagaaac ucgaaagagc caucuuuuau cagcgcccuc uuaagagcca gaaaggacug      780

gucggcaagu gcacccugca aaacaauaaa caucgguguc ccauuucuag accugaguuc      840

gaagaguuuc gcauguggca guucaucaac uccauuaagu acaagaauag cgaugggaaa      900

uuugacuucc ucaacaagga acagcgggag aauaucaucc ccgcuuucgu gucuaacaaa      960

aaugccacau uugaguucuc caagauugag aaaauccuga acaagggagu ucacauuuau     1020

caguuuaauu acaagagcaa ccauaccauc ggcucuaauc aaacaaugau uaacuucaug     1080

gauguguuug acaucgauag aaaacuuacc aacgcguggc ugcucugcgu caauaagauc     1140

uaugaacugu acacggccaa aacaaagaaa gacggcuccc ucaagagcaa auccgaagug     1200

auuaccgaug uguggcacgu ccuguucaca uuugacaacg aggagaaucu gaaggaguuc     1260

gcucgcaaca aacuucugcu cgacgaaucu agcgcccaga aauuuuccaa gaucaaucug     1320

aaacaggauu acagccagcu uucucugaag gcuauuaaca aaauccuccc uuaucugcaa     1380

gaagggcuga uuuacuccua ugccguuuuc aucgcgaaua uugagaacgu gaucggaaag     1440

agcaucugga auauuaagga aaaccagcag aucauucgga ccucuaucaa aaagauuauc     1500

gacaaucaca aaaccgagcu cgauaucaac acaauuacga auagccugau caacgacuuu     1560

aaggugaauu acaacaacgc ccauaaagag uauaaggucg augacauuga ucuuaagaau     1620

aucagagaga aaauuaucaa guacuauggc aacaaaaccu ggaauugucu gccggaagac     1680

gaaaagcucu acgugaaaga gaagguugaa aagcuguacg agaaacaacu gaaguccggg     1740

aaacgcggaa ucuucauuga gaagauccgg cuugaugaca gaauugaaaa cuuucucaaa     1800

gauaauuucg acucuagcga gcugaaccug aagaaacucu aucaccccuc cgacgugagc     1860

gucuacaagu cugugaagau cacagaucug ggccuucgcc ugcucggguc cccuaugauu     1920

cccagcauca aaaauccuau ggcuaugcgg gccaugcauc agcugagaaa gcugaucaac     1980

caccuuauua ucgaaggagu uauugacgau ucuaccauca uuaaugugga gaugucccgg     2040

gaacucaacg acgcuaauau gcgcaaagcc aucgagagau aucagcggca gagagccaca     2100

gagaacgaag aguucaagac caaaauuaag gaacuguaca agaaagagug cggcacggau     2160

uuugagccca auaacgacga uaucaugaag uaucgccugu gggaggaaca aaaucacaaa     2220

ugucucuaca caggcaagag caucgggauu ugcgacuuca ucggauccaa cccggcuuuu     2280

gaaauugagc auaccauccc ucggucucug agcuaugaua acucccuuga aaauaaaacc     2340

cugugugaga aggaguucaa ccagcagguc aagaaaaauc gcauucccuu ugaacucucu     2400

aacuacaagg agaucaucag cagaauuaau aacugggaaa ucaaagcgga ggaacuguuc     2460

aagcagauuc aaaaagugaa gauccagucc agaauggccg cuacaaagga gauuaaagac     2520

aaugccaucc agcggaagca ucugcuucag auggaguuua acuacuggaa uggcaaacuc     2580

agccgcuuca ccagagaaga ugucccugag ggguuuaaga acucucaacu gguggacauc     2640

ggaauuauca caaaauaugc ccgggcuuac cugaagacgg uuuucaauaa cgugaauacc     2700

gucaagggcu ccacaguggc cgaauuucgc aacauuuggg ggcuccagga guauuucgag     2760

aaaaaggaua gaagcaauca cauccaccau ugcauugacg cgaucaccau ugcuugucug     2820

acaaaagaca aguacgauuu ucuugcccac cuguaucacc ucgacgaaca gaacaaucgg     2880

gaugccguuc aucgcgcuau gaucaaaucu aagcccuggg agaacuucgu gcaggacguc     2940

aaagaaaucg agaaccaaau ucuggugucc cacuacaccc ccgauaaucu gccuaagcag     3000

agcaagaaaa uccuuagaaa gcggggcaaa auucagaaga acgaaaaugg agaccucauc     3060

uaugagaaag gcgaugccgu gcgcgggucu cugcauaagg agagcuucua cggagcuauu     3120

aagagagaaa ucaucaacaa aaagggcgag acggaagaga aaguucugua cgucaagcgg     3180

aaaccgcucg aauuuuccga ccugggguuc aagaccauua gcgagcuuga gaauaucgug     3240

gaugacgccg ugagagauaa gguccugaac gcggugaaag acaagggacu caaacaagcc     3300

cugaaugaac ccauuuggau gaacgaggaa aagcagaucc cuauuaaaaa gguucgcugc     3360

uauacacccu cugugaccaa uccuaucaac cugaagaaac accgggauaa guccaaacac     3420

gaguacaagc agagcuauca ugucacaaau gacggcaacu accuuauggu gaucuaugaa     3480

gggaaagacu cccaggagaa gguuaaaaga gauuacgaga uugugucuaa ucugcaagcu     3540

ggcgaauauu uuaagcagag cguccacgga gugcucaagg cccagggcau caacaaucug     3600

gaggggcuca uucccaaauu caaggaaucc ggcaaaaaca uccugccgcu uaaguacauu     3660

cugaaaaccg gaacgauggu uaucuuuugg gagaacuacc cugaagagau uugggacauc     3720

gauaagaauc agcucuauaa gcgccuguuc aaagugacac aauuugaguc ugacgggcgg     3780

auccaggcua gauuccauca gauugccaag cccgauaacg aacugaccaa agucagcgag     3840

cuugacuuuu ccaauaucgc ggaaaagauu cgccugucua aaggaaaccu caauaugcug     3900

gugaacggcu acgauuucaa gaucaaugug cucgggcaca uugagaagcu gagcuga        3957


<210>  4090
<211>  3957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4090
augggcagca acaugcugaa gaaagacuac uccaucgggc ucgauauugg aaccaauucu       60

gugggccacg ccgucguuac agacgauuau aaggugccca cgaaaaagau gaaaguguuc      120

ggggacacca gcaagaagac aaucaaaaag aacaugcugg gaguccuucu guuuaaugag      180

ggccagaccg cugccgauac aagacucaaa cgggggaccc gcagacggua cacacgcaga      240

aagaaccggc ugcgcuaucu ucaagaaauu uucgcgccug agcuggcuaa aguggacccg      300

aauuuucucu acagacugga agaguccucu cugguugccg aagauaagaa guaugacgug      360

uaccccaucu ucggaaaacg ggaggaggaa cuccuguauc augauaccua caagacgauu      420

uaccaccuuc gcagcgcccu ggcuaacaau gaccagccug ccgaucucag acuggucuau      480

cuugcucugg cccauaucau caaauaccgg ggcaacuuuc uccuggaggg cgaaauugac      540

cugcgcacca cagauaucaa uaagguguuc gcggaguuuu ccgagacccu caacgaaaau      600

ucugacgaga accuggggaa acuugacguu gccgauauuu ucaagaauaa cacauuuagc      660

aaaaccaaga aguccgagga acugcucaaa cugagcggag cuaagaaaaa ucagcuugcc      720

caccagcugu ucaagaugau ggugggcaac auggggucuu uuaaaaaggu ccucggaacg      780

gacgaggaac auaagcuguc cuucggcaaa gauacauaug aggacgaucu gaacgaccuc      840

cuggcugaag ccggggauca auaccuugac aucuuugugg cggccaagaa aguguaugau      900

gcugccauuc uggcuagcau ccucgacguc aaggauaccc agacaaaaac cguuuucucu      960

caggccauga uugagagaua cgaggaacac cagaaggacc ugaucgaacu uaagcgggug     1020

uucaaaaagu aucugcccga gaaaugccac gacuuuuucu ccgagccuaa gaucagcgga     1080

uacgccggcu acauugaugg caaagugucu gaagaggacu uuuauaagua caccaagaaa     1140

acacucaagg ggauccccga aacggaggaa auucugcaaa agaucgaugc uaauaacuau     1200

cugcgcaagc agagaaccuu cgacaaugga gcgauuccuc aucaggucca ccucaaagag     1260

cugguggcca ucguugagaa ccagggcaag uacuauccgu uucuucggga aaauaaagau     1320

aaguucgaga agauccugaa cuuucgcauu cccuacuaug uggggccucu cgcuagagga     1380

aauuccaaau ucgccuggcu gacacgggcc ggcgaaggga agaucacccc cuacaacuuu     1440

gacgagauga uugauaaaga gacaagcgcu gaagacuuca ucaagcauau gaccauuaau     1500

gaucuguacc uuccuacgga gcccguccuc ccgaaacacu cccugcugua ugaaagauac     1560

acaaucuuua acgagcucgc cggagugcgc uauguuaccg aaaauggcga ggcgaaguac     1620

uucgacgcuc aaaccaagcg gucuaucuuu gagcuguuca aacuugauag aaaggugagc     1680

gaaaagaugg ucauuaagca ccugaaagug gucaugccug ccauccgcau ucaggcccuc     1740

aagggccugg acaacgggaa guucaaugcu uccuauggaa cauacaaaga ucugguggac     1800

augggcguug cccccgagcu ucucaacgac gaagugaaua gcgagaagug ggaagauauc     1860

auuaaaaccc ugacaaucuu ugaggggcgg aagcugauua aaagacgccu cgagaacuau     1920

cgggacuucc ugggagaaga uauccuuaga aagcugucuc gcaagaaaua cacgggcugg     1980

gggcggcucu ccgcuaagcu gcuggacggc aucuacgaua aaaagaccca uaaaacaauu     2040

cuugacuguc ucaugaccga ggauuauagc cagaacuuua ugcaacugau caaugacgau     2100

aacuacucuu ucaaggaaac aauuaaaaau gcccagguca ucgagaagga agagacccug     2160

gcgaagacgg ugcaggagcu cccuggaagc cccgccauua aaaagggcau ccugcagucc     2220

cuugaaaucg uugacgagau uaucaaagug augggguaua agcccaaauc uauugucgug     2280

gaaauggcua gagagaccca aaagacacac ggaacccgga agcgcgagga uagagugcag     2340

cagaucguug aaaaccugaa agacgccaau gagcucccug aaaagcugcc gagcaacgcu     2400

gagcuguccg augaacggaa auaccuuuau ugccuccaga auggcagaga cauguacaca     2460

ggggcgcccc uggacuauga ucaucugcaa uuuuacgacg ucgaucacau uaucccucag     2520

agcuuccuca aggacgauuc uaucgagaac aaagugcuga ccauuaagaa ggagaaugug     2580

cgcaaaacga acggacuucc cuccgaagcc gucauccaga agaugggcag cuuuuggaaa     2640

aagcugcucg acgccggggc uaugacaaau aaaaaguacg auaaccugcg gagaaaucug     2700

cauggcggac uuaacgagaa gcugaaagaa cgcuucauug agagacagcu cguggaaacc     2760

cggcaaauca caaaguaugu ugcccagcug cucgaccagc gccugaauua cgauggcaac     2820

gggguggagc uugacgagaa aauugcuauc gucacccuga aggcccagcu cgccucucaa     2880

uuuagauccg aguucaaacu gcggaaggug cgcgcucuga acaaucuuca ccacgcgcau     2940

gaugccuauc ugaacgcuau cguugccaau cucauuaugg ccaaguaccc ugaacuggaa     3000

cccgaguuug uguauggaaa auacagaaag gcuaaauuca agggccucgg gaaagccacc     3060

gcgaagaaca cacuguaugc uaauguccuu uacuuucuga aagaaaacga gguguacccg     3120

uucugggaca aggcccggga ccucccuacg aucaagcgcu aucuguacag agcccagguu     3180

aauaaagugc ggaaggcuga gcgccagauu ggaggcuuca gcgaugaaau gcuggucccc     3240

aaauccgacu cugggaaacu ucugccuaga aaagagggcc ucgaucccgu gaaguaugga     3300

ggcuacgcca aggcggugga aagcuaugcu guccugauca ccgccgacga gguuaaaaag     3360

gggaaaacaa agaaagugaa gacccucgug aacauuccua ucauugauuc caagaaauac     3420

gaggccgacc ccacagcuua ucuggccucu cggggauaca ccaaugucac gaacagcuuu     3480

auccuuccga aguacucccu gcuggaagau ccugagggca gacgccggua ucucgcuagc     3540

uucaaagaau uucaaaaggc caaugagcug auccuucccc agcaccuggu ggaacuccug     3600

uacuggguua acgcgaaaga cggggagcag aagcucgagg aucauaaggc cgaguucaaa     3660

gaacuguuug acaagauuau ggaauuugcu gauaaauaug uggucgcccc caaaaauucu     3720

gagaaaauca gacgccuuua cgaagagaac caggacgcua caccuaugga gcugggaaag     3780

aauuucgugg aacugcuccg guauaccgcc gauggcgccg cuuccgacuu caaauuuuuc     3840

ggggagaaca uuccgagaaa gcgcuacaac agcgcgggcu cucugcuuaa uggaacccug     3900

aucuaucaau ccaagacagg ccucuacgaa acccggauug accuggggaa acucuga        3957


<210>  4091
<211>  3960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4091
auguucagcc agagaaacaa ucccuacugc gagcugcucu uuaagaucau ugugaccaac       60

aucgaagaga aaaaucugga agucauuaug ggcauccgga acuccgacau uuucuuuauc      120

ucuaauuuca ugugguauca aaaacuuauc augaugauua aaaacauccu ggggcucgau      180

cugggaacaa auagcauugg cugggcccuu aucgagguug acgaaaacaa uauuccuauc      240

cgcauuaucg cuaugggguc cagaaucauu ccgcugucua cgagcgauaa ggaggaguuu      300

cagcggggac ucuccaucac caaaaaccag aaucgcacag ugucuagaac ccagcggaag      360

ggcuacgacc gcaagcaacu gagacggagc gaucugaaaa agauucucaa aaaguauaac      420

aucuuccccu ccgaggagcu gcuuaaacug ccuaugcucg accuguggaa acuucgcagc      480

gauucugcca aucccaacga agacauuucc gcggagcagc uggggagaau ccucuacaug      540

cugaaucaga agcggggaua uaaaagcgcu cgcucugagg ccaacuccga uaagaaagac      600

acagauuacg ugcaggccgu caagggcaga uaugcucaac ugaaagaccg gcgccagacc      660

aucggccagu acuuuuacga ggaacucagc acagccuucu cugauaagaa caaucagcaa      720

uauuuuagag ugaaagaaga gguuuacccu cgggaggcuu auauugaaga guucgacacc      780

aucauuaacg cccagaagga caagcacggg uuucugacgg auaaagugau ccaucagcuu      840

agaaaugaaa uuaucuacuu ccaacgcaag cugaaauccc agaagggacu cgucagcauc      900

ugugagcugg aaggcuuuga gaaaaccgug uaugacaagg aaaagaacaa aaauaagguu      960

guguucgucg ggcccaaagu ggcgccuaag acauccccgc uuuuccagcu gugccggauu     1020

ugggaggugg ucaacaccau cucucucaaa auuaagaauc ccgagggaag caaguacaaa     1080

uggggcgaaa agguuccuaa ccugaaacag aaggagaucc uggccgauua ucucuuuaaa     1140

aaugaaaacc uguccuucag cacacuucug gagauucucg aacugaagaa ggaggacgug     1200

uacgugaaua aacaaauccu uaaggggauu aaaggaaacg aggcuuacgc cgcucugcac     1260

aauguccucg gcgaucauga acugcugaac uuugacgugu cuaucaucac cuccgaucac     1320

acggccauuc ucguugacaa gaaaacaggc gagauccugg aagagagagc ggggcuugag     1380

cuggaugcca gccucgaacg cgagccccug uaucaguucu ggcauaccau uuacucucug     1440

aaggaccuug augaauguaa aaaugcucuc aucaagcggu uuggacugga cgaggacauu     1500

gccgaaaagc ugagccacau cgauuucaac aaacaggcuu uuggcuccaa gucuaacaaa     1560

agcaugagaa agauccuccc uuuccugaug gagggguaua auuacuccga gagcugcucu     1620

cuugccggau auaaccacuc caauagccug acaaaagacg aacgcgaguc ucagauuacc     1680

cucgaacggc uggagcugcu ucccaagaac aaucucagac aaccgaucgu ggaaaagauu     1740

cugaaccaga ugaucaaugu cgugaacacc auucuggaga aauacggcaa gccuuccgag     1800

auccgcguug aacucgcccg ggagcugaaa cagagcaagg augaaagaaa ugacacagau     1860

cuucagaacu cccgcaauaa aaagcugaac gaggaaaucg ggaagcggcu cgcugagcug     1920

ggacugcccg ugacgaaaag auauauucaa aaguacaaau uuaucuuccc uucucuuagc     1980

aagaaucuca aagacgcgca ugucaacaau caguguauuu acugcggcga gaccuuuaac     2040

cugacagaag cccugaacgg cgaugcuuau gacguggauc acaucguccc caaggcccuc     2100

cuguucgacg auucccagac caauaaggug cuuguucauc ggaaauguaa cucuacaaag     2160

accaaucaga cggccuacga cuauauuaca gcuaaagggg aggaagaggu gcgcaucuac     2220

agcgagagag ucgaugacug guuuaagaaa ggaaucauuu ccuauagcaa gaugcaacgg     2280

cugaaagugu cucacaagga auaccucgag agaaagaaac ugggcaagga aaccgagacc     2340

gacaaaaagc ugugggaaaa cuucaucgau cgccagcuuc gggacacaca guauauuucc     2400

agaaaaagcc gcgagauccu ccaaaagguu ugcaauaacg ugaccacaac ggaaggggga     2460

gucaccgcca agcugagaaa ucuguggggc ugggaugaca uucucaugaa ccugcagaug     2520

cccaaauaca aggaacuugg gcagacaauc accaaagagu ggacaucuga acacggcaag     2580

cggaaacauc agaaggagga aauugagaau uggaccaagc gcgaugacca cagacaucac     2640

gcgaucgaug cucugguggu ggccuguacg aaacaaggau ucauccagcg gauuaacacc     2700

cucaauucca gcgagacaaa ggaccugaug aucaaagaag uugaggaagc caagauugag     2760

uuuaacgaaa agaccacacu gcuugagaag uaccugaaga cccagaaacc uuucacgaca     2820

cgcgagguca ugaaggaagc ugauaaaauc cucauuucuu uuaaggccgg caaaaaggug     2880

gcuaccauca caaaguucaa agccaccggg aagaauaaag agaccggagu gaucgucccg     2940

agaggcgcgc ugcaugaaca gagcguguau gggaagauua aaguuaucga gaaggacaaa     3000

ccccucaagu accuguuuga gaacccugau aagauuguca auucccggau ccgcgaccuu     3060

guggaaacaa gacugagcga gaacgaaaac aauucuaaaa aggcccucuc cacggugaaa     3120

aagaacccca uuuucuuuaa ugaggaaaag aacgagaucc uggagaaggc uagcugcuau     3180

aaugaugcca ccguucugaa guacaaacuu caaaaccuga aggcuaauca gguggacgac     3240

aucgucgaug aacgggugaa auuccucguu aaggagcgcc ugacacaguu uggauccaaa     3300

gaaaaggagg ccuucaagga cauucucugg uuuaaccagg aaaaacaaau cccuauuauc     3360

accgugagac uguucgcgcg gcccgaugcc aauaaccuuc agauuaucaa gaaagacaag     3420

aauggcaaag auauuggguu uguccugaca ggcaacaauc accacaucgc uaucuaugag     3480

gacaaggauu cuaagcucgu gcagcauagc ugugccuucu ggcacgcugu ggagcgcaaa     3540

aaguacaaca uuccggccgu caucaaaaau uccgccgacg uuuggucucu gcugcuugau     3600

aaggaacugc cuguccucuu ccuggacaaa cuccccgcug augagcugaa ccuuaaguuu     3660

agcaugcaac agaaugaaau guucauucug ggacuguccc aggagggcuu ugacgaagcg     3720

aucaaaaaca acgacaaguc ucuccugagc aagcaucuuu aucuggugug guccauuagc     3780

aauaacgauu acuacuucag acaccaccuc gagaccaaaa auacggagcu gaagaacauc     3840

ccuacagcca aagacucuaa gcgguauuuu agauucaaau ccgugggggc ucucguggga     3900

aucaauccca uuaagguucg ccugaaccau cuuggcgaaa ucaccaagau uggggaguga     3960


<210>  4092
<211>  3960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4092
augggcuaca acaucgggcu ggacauugga accggcagcg uggggugggc cgcucucaca       60

gaugagggaa agcuggccag agcgaaaggc aagaaucuua ucgggguccg gcuguucgac      120

uccgcucagu cugccgccca acgcagaagc uaucggacga cccgcagacg gcucucccgc      180

agaaaauggc ggcugcgccu ucuggaaaac auuuucucug augagauggg aaugaucgac      240

gaaaauuucu uugcuagacu caaguacagc uauguucacc ccaaagauga ggugaacaau      300

gcccauuacu auggcggcua ccuguucccu acacagcagg aaacccacga cuuucaugag      360

aaguuccaga caauuuauca ucugcggcuc aagcugauga ucgaggauug caaauuugac      420

cuucgcgaaa ucuaccuggc uaugcaccac auugugaagu acagagggca cuuccucaac      480

ucccaaucua aaaugaccau cggagauagc uauaauccgc gggacuuuca gcaggccauu      540

cagaacuacg cggaggccaa gggccugauc uggucccuua augaugcuca agaaaugaca      600

gacguccugg uggggcaggc cggauucggc cucagcaaaa aggcuaaggc cgagcgccug      660

cugucugcgu uuuccuucga uaccaaagag gacaagaaag ccauucaggc uauccucgcc      720

gggauuguug gaaacacgac cgacuuuaca aagaucuuca auagagaacg gagcggcgau      780

gagcugaaaa aguggaaacu uaagcuggac ucugaagcgu ucgaugagca gucccaagcc      840

aucguggacg aacucgauga cgaugagaug gagcuguuua acgccauuag acaggcuuuc      900

gacggcuuua cccuuaugga ucugcucggg gaccagacaa gcaucucugc ggccaugguc      960

aagcgcuauc aacagcauca cgaugaccug aaaaugguga aggaaauugc uaaaaagcag     1020

ggacuguccc aucaggacuu cagcaaaauc uacaccgccu uucucaagga ugacacggau     1080

aagggcauga aagcccugcu ugacaaggcu gaucuggccg acgauguucu cguggagauu     1140

caacagcgga ucgaauccca cgacuuccug cccaaacaga gaacaaaggc uaauucuguc     1200

aucccuuauc agcuucaccu ggcugagcuc gagaaaauua ucgaaaacca agggaaguac     1260

uaucccuuuc ugcuggauac cuucacaaau aaggccggag agaccauuaa caaacucgug     1320

gaacugguga aguuucgcgu cccuuacuac guuggcccca uggugaccgc cgcugacgug     1380

gagaaagccg ggggagaugc uacaaaccau ugggucaagc ggaaugaagg cuaugagaaa     1440

agcccuguga cgccguggaa cuucgaccag guuuuuaaua gagaucaggc cgcgcaggac     1500

uucaucgacc gccuuaccgg cacagauacc uaccugauug gggaacccac acuccugaag     1560

aacucccuga aguaucaacu uuuuaccgug cucaaugagc ugaacaaugu caaaaucaac     1620

ggacacaaga ucgacgagaa aacgaagcau gugcugauuc aggaucucuu caaaagcaag     1680

aaaacaguuu cugaaaaggc caucaaggac uacuaucugu cccagggcau gggggagauu     1740

cagaucgugg gacuugcuga uaaaaccaag uucaauagca accugucuag cuacauugac     1800

cucuccaaaa ccuuugaugc cgaauuuaug gagaauccug cuaaccaaga gcugcuggaa     1860

aauaucauuc agauccagac agucuuugag gacgugaaga ucgccgaacg ggagcuucaa     1920

aaacucgcgc ugcccgauga acagguccag cagcuggcca agacccacua uacaggcugg     1980

gggaaccucu cugacaagcu gcuuagcacg ccuauuaucc aagagggcuc ccagaaagug     2040

agcauucuga auaagcucca gaccacaucu aaaaacuuca uguccaucau uaccgauaac     2100

aaguuuggag uucagcaaug gauccaggag cagaauacag cugaaaccgc cgacagcauc     2160

caggauagaa uugacgagcu gacgaccgcu cccgccaaca aacgcggcau caagcaagcc     2220

uucaaugugc uguuugacau ucagaaggcu augggggagg aaccgaaccg ggucuaccuu     2280

gaguucgcga aagaaacaca aaauucugug agaaccaacu cccgcuacaa ucggcucaag     2340

gaucuguaua aaagcaagac acuguccgac gauguuaaag cccucaagga ggaacuggag     2400

ucucaaaaga gcucccuuca gucugaaaga aucggagacc ggcuguaccu cuauuuucug     2460

cagcagggca aagauaugua caccgggcaa ccuauuaaca ucgacaagcu gagcacggau     2520

uaugacaucg aucauauuau cccccaggcu uacacaaaag acgauuccau ugacaaucgc     2580

gugcuuguca gcagaccuga gaacgcccgg aagucugacu ccgccaccua uacaaccgaa     2640

gugcagcaga gcgcuggagg ccucuggaaa ucucugaaga augccggguu caucucccag     2700

aaaaaguacg auagacugac caagggcgga gacuacagca aaggccagaa gacaggguuu     2760

auugcgcgcc agcucgugga gacgcggcag aucaucaaaa acguugcuuc ucugauugag     2820

agcgaguucu cccaaaccaa ggccgucgcc aucagaagcg aaauuacagc ugauaugcgc     2880

agacuugugg ccaucaaaaa gcaccgggaa auuaauucuu uucaccaugc guucgacgcu     2940

cugcucauca ccgccgccgg acaguauaug caggcucgcu accccgauag agacggcgcc     3000

aacguguaua augaguucga uuacuauaca aacaccuacc ugaaggagcu gcggcagucc     3060

agcuccucua gccaaguccg cagacuuaaa cccuuugggu ucgugguugg aacgauggcu     3120

aagggcaacg aaaauugguc cgaggacgau acacaguauc ugcggcacgu gaugaacuuu     3180

aaaaauauuc ucaccacccg cagaaacgac aaggauaaug gggcccugaa caaagaaaca     3240

aucuacgcgg ucgacccuaa ggccaagcuc aucggcacca auaaaaagcg gcaggaugug     3300

ucucuguacg gaggcuauau uuacccguau agcgcuuaca ugacacuugu ucgcgccaac     3360

gggaaaaauc ugcucgugaa ggucacgauc uccgcugccg agaaaauuaa gucuggacaa     3420

aucgaacuga gcgaguaugu gcagcagaga cccgagguua agaaauucga aaagauucug     3480

aucaacaaac uugccaucgg ccagcuggug aauaacgacg ggaaucucau uuaccugacc     3540

uccuaugagu uuuaccauaa cgcuaagcaa cucuggcugc cuacagaaga ggcggaccuu     3600

aucagccagc ugaauaaaga uucuuccgac gaagaucuca uuaagggauu cgacauccug     3660

accagccccg ccauucugaa gcgguuuccu uucuacgagc uugaucugaa aaagcucguc     3720

aacaucagag acaaauuuau cgcuguggag aacaaguucg auauucugau ggugauccuc     3780

aaagcccugc agcuugacgc cgcucagcaa aagcccguca aaaugauuga uaagaagucu     3840

gccgacugga aagauuaucg ccagcggggc gggaucaagc uguccgacac aagcgaaauu     3900

aucuaccagu cuaccacggg cauuuuugag aaaagaguua agaucuccaa ucugcucuga     3960


<210>  4093
<211>  3963
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4093
augaccgagc agcaaagacc cuacaacauc ggccuggaca uugggacaag cuccaucgga       60

ugggccguga cggaucagaa ugacaagcuc cugcacauuc ggggccauaa cgggaucgga      120

guccgccuuu ucaaagaagg cgagucugcu gccgaaagac ggggguuucg caccacaaga      180

cggcgccuga gcagacggcg cuggagacuc cgguuccugg aucgcauuuu ugacgcggag      240

cuugcuaagg uugauccuaa uuuccuggcc agacucaaac aguccaacau cucuccgcug      300

gaccccaaua agcagauguu uggaaacauc cuguucgaug acgcccaauu ugaugaccag      360

aaauuccacc agcaguaucc uaccauuuac caucuccgga gcgcucuggc ccaacccgau      420

gcuccucagg ccgacgugcg ccuuaucuau cuggcgcucc accauaugau uaaguuuaga      480

ggccacuucc ugaauggcgc cgcuguggaa aaguuucacg ggggacagcu ucaucuggag      540

gccgaguucc aggaacucaa ccaaaucuuu uccaaucagc agcgggauca cguccaacug      600

cagacaucua gccuggacga gguggcuacc auucucaaag acaaccagag aacacccucc      660

gaucgccagc gggaacugag cgccagacuu uucguuccug uggacaagac caucgauaaa      720

gagaauaaga aacuggcgac ggagcuccug aaggccgucc uuggccugaa ggcuaaauuc      780

gacgugauuc ucgggguuga agccaccgau ccgcaggcuu uuaagcugca auucucuucc      840

gacgauuuug acgauaaaau cgccgagcug gccagcgaac ucacagacga ggcuaacgaa      900

auccugaccg ugcuucagcg ccuguacuuc ucucucaauc uggcggauau ucuuacaaac      960

cggcagacca agcaagucgc cgacuccauc agcacggcua ugauuaauag auaugacgau     1020

cauaaacacc accugaagau gcucaaagag cuggagacac aggugaccca ggaacagcgc     1080

gacggacuga agaaggccua cgaugaguau aucgacggcg auaaacccac aauugacacc     1140

uuuuacacca acgugaagaa aaaccucgau gacucugaac uggccaauca aaucaagucc     1200

cuuaucgauc ugcaggcuuu caugccuaaa cagcggagca aggagaacgg guccauuccc     1260

caucagcucc accaaagaga acuggaccag cugaucgagg uccagggaaa guacuauccu     1320

uggcuucucg ccgcgaaaga uaagcuggac gcucugauug ccuuucgcgu ucccuacuau     1380

gugggcccga ugaucgaucc uaaugagucu gacgaccccc aaaacacaaa auucgccugg     1440

auggugcgga aggaagcugg cgagauuacg ccuuggaauu uugaagagaa agucgauaag     1500

accgggagcg ccacaaacuu caucaagaga augaccacaa ccgacacgua ccuccuggga     1560

gaacccgugc uucccaaaaa uucccugcuc uaucagcagu uuacaguucu gaacgagcug     1620

aauaaacuua aaguggaugg caaggcucuc accgucgacc agaaaaagua ccuguaugag     1680

caugugugca agaaacaacg ccggguuacc cugaagaaac ucagcgauug ucugauggcc     1740

agaggggacu ucccuauggg agcgaucuuu acaggcacca cagaucagaa gaacaugaau     1800

aacacgcuua aaaccuacca ggacuuccag gaaauuuucg gggaugagau cgacaauccg     1860

gaucggcaag uggacuuuga aaagaugauu gaguggucua caaucuucga ggaucgcgaa     1920

auuuuuaaac ugaagcucgc cgagcugaac uggcugaccu ccgaacagcu ugagagaguc     1980

agcaagaaac gguaccaggg auggggcaga cucucucaga agcugcugac acacaucgug     2040

aaugaaaacg gcgagcgcau uaucgaccaa cucuggaaca ccaaucagaa cuucauggag     2100

aucauggggc aggacggagu caaacaagcu auucaggaac auaauggcgc caacguggcu     2160

cagacgggga ucaaugagac cauugaugaa cuguauacaa gcccccagaa caagaaagcc     2220

auccggcaaa uucuucuggu uguggacgau aucaagaagg cggucggaua cgccccugcu     2280

aauaucauga uggaguuugc cagagaagac cgcgaugacc acagacucau ugcuucccgg     2340

gcccgccagc uggagaaaac cuaucaggag aucucuaaga acugguucga uaaugaaagc     2400

gugacacagg agcugaaaga ccaaauuaag aacaaaacca aguuuacgga uagacuuuac     2460

cucuauuuca uccagggcgg gauugaccug uacacaggcc acccccugaa uaucgaucag     2520

cucuccagcu augacaucga ccauauucug ccucagucuu uucuucugga uaacucccuc     2580

aauaaccggg uucugaccag ccaaucugaa aaucgcgugc acaaggagga cagacugccc     2640

ggagaaacag ucgccaacaa aaugcggccg accuggaaga ugaugcgcga gcagggccuu     2700

augacccaga gaaaauacaa caaucugaca cucaagccug aucagaucuc caaauucaac     2760

aaucgggggc aauuuauuaa ccgccagcug guggagacga gacaggugau caagcucgcu     2820

gcggacauua ugaaucaaaa guaccaggcc gaucagacca aaguugucac agugaaggcu     2880

aaccugaccc aucacaugcg ggaaaaauuc aauuuuuaua agagcagaaa cgugaaugac     2940

uaccaccaug ccuucgaugc cuaucuuaca gucuucgugg gaaacuggcu gcucaaagag     3000

uaccccaagc ugcagccuua uuuuguuuac ggcgacuucg cuaaaaccgg gacgaauaac     3060

cugaaguccu uuaauuuccu uuaucgcuuu gaacgggauc acaagcugaa cgacaaagga     3120

gccaucgaua cagacaagga ucaacagcuc ggcuacauga aaagaaucua ccgcuucaag     3180

aaaaugcugg ugaccaagca gcucgagacc aaugaggggg cgcuguauaa gcagacaauu     3240

uacaaagcuc ccgccaacga aaagaacacc aaaaagaauc ggucucuuau caacacaaag     3300

aagaauagac cuagcgacau uuauggcgga uacucccaau cugucacggc cuauaugagc     3360

cugguguuuu ucaagaacac cuuuaaacuc aucaagaugc cccugguugc ugccaaugcg     3420

auuaaaaacu cccugagcuc uuaccaugac acaauccuua agauucuggg cuccaaugag     3480

uucaaagugc ucaucccgaa ggucaagaug aaccagcuga ucaaagaagg ggauagccuc     3540

uauaccgugg uuggagugua caagcacaau gcucagcagc ugauucuuuc ugacaacucc     3600

augggcauca uuacaaaacc uauccgcgag auuugcgaua gcgaccugga uucugucuuu     3660

aaugaacugg uggacaacau cgauaaguac cucaaucugu auaaaaucga ggacaaacuu     3720

accauuaagg agaaguucua cgcccugaag aacaaagaaa gcaaguucaa auguaucucc     3780

gauauucucg uggggacgca uugcaaugcc ggacggagca accugaagau cauuggccuc     3840

ucuuccuauu gggggauguu uagcggcaau aacgacggac ugaaaaucaa cgaaaauucc     3900

caacuuaucu accagucucc caccggccug uucgagcaca gagucaaggu uagcgaucug     3960

uga                                                                   3963


<210>  4094
<211>  3972
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4094
augaaggacu ucuacaucgg ccuggauaug gggaccgaca gcgugggaug ggccgucgcu       60

gaugaggacu auaaccucug caaauuuaag ggcaaagcca uguggggagu uagacuguuc      120

gaugaauccg cgacagcugc cgagcggcgc ggauuuagaa cggcccggcg cagagcucag      180

cggaagcgcc aacggauucu uuggcugcag augcuuuuca augaagagau cucuaaaaag      240

gacauugccu uuuuccagag acugaacgaa agcaagcucu accuggagga uaaauccacc      300

aaugugcccu augcuguguu ugccgacgag ucuuucacag augucgacua ccacaagcuc      360

uauccuacca ucuaccaucu gcgcaaagaa cuggugcaga gcgaugagcc gcacgacguu      420

agacucgugu aucuggcgcu ucaucaccug auuaagaacc ggggccauuu ucucuucgaa      480

aaugucgggu ccgauuuuga gaaagagucu agcuucgaag ugcuguuuaa gaaccuucaa      540

aauuacaucc ugggagagua cgaacucgcc cuggagugua ucagcccccu gcagauugcu      600

gacauccuca aggacaaauc caagcgcaaa acaaccaagu cuuccgaaau uugcaaacug      660

cuuggcauca gcaagaaagg ccagccuaga cugaacgcca uuguucuccu gauguguggg      720

cagucuguga aacuugcuga ucuguaugga gacgagaauu acaacaagga ggaaaaaucc      780

agcaucucuu ucgagaaggg cuaugaagau aaugaggaaa cauaccggga gcuccugcug      840

gagcgcuucg aacucauuga gaaaaucaag gcccuguaug acugggcgau ccuugccgau      900

auucugaaag gggaaaccua caucuccuuu gcuaagguca agacguauga cgagcacaga      960

cacgaucucc aacagcugaa agccuucgug aaggaacggu gcagaagcga cuacaacgag     1020

auuuuucgcg agaccuccga uaaacuuaau aacuacacag cuuauugugg aaaguucaaa     1080

gaaaauggcc ggaacggggu gauccugcau ucugccaacc agaccgacuu uugcaaguac     1140

cucaagagca aauucgauaa gcuggacaaa uccggauaug aggacauguu ugaaaagauu     1200

gagaauggca gcuucaugcc caaaaucguc guuaaggaua acggcgugau cccuaugcaa     1260

gugaaugagu cugaagucag agccauucug aagaacgcuu ccggguaccu cgacuuucug     1320

aauaaccagg auaaagacgg aaucacagug gcggauaaga uucuucagau cuucgaguuu     1380

cgcauucccu auuacguugg cccucugaau gugcacagcg acaacucuug gcucguccgg     1440

aaagauggga agaucuaucc guggaauuuc aacaaaaucg uggacguuga uaagagcgcc     1500

gaagaguuua uuaauaaacu gaccgcuaag uguacguacc ugcccgacag agaugugauc     1560

ccuaagaacu ccauucuuua cucucgcuau augguccuca augaacugaa caaucugaaa     1620

cucgacggaa agaaacccga ugugagcuuc aagcaggccg ucuucaacga ccuguuuaug     1680

caucggagaa aagugacaca aaaggcccuu cgcgaguaca ucaaggcuca gaccggcaca     1740

gagccugaaa uuaccgggau cgacggagau uucaaagcca acaugcggcc cgcgauugag     1800

cuggcuggcu auaaucucuc cgaaccggac aaggaggaaa ucaucaaagc cauuaccauc     1860

uuuggcgaug acaagaaacu gcugaagaga cgcauuaagg auaaauacag cgagaugcuu     1920

ucugaggacg aaaucaagaa aauuugcaag cucaaauaua aggauugggg gcggcugucc     1980

agagaguucc ugacagaaau cuuugacguu gauacggcca ccggagagug uaaggacaac     2040

aucauuaaua cacucuggaa caccaaugau aaccugaugg aacuucuggg cgcuaaauac     2100

aauuucacac agagcaucga gcaggccauu ucuaagucca cccacaaagc uagcaucgag     2160

aagauggugg aagacaugua ugucuccccu aaagugaagc ggacgguuuu ucaaucucuc     2220

aaaauuauga aggagaucgu gaagaucaaa gggcacgccc ccaagaaagu cuucguggaa     2280

augacacgcc augauggaac caagggcgac aacgggagaa ccaaaagccg gaagaagcag     2340

cuggaggagc uguacaaaaa gugcaaagaa gacuccacag agcuuuggga ugaacucgag     2400

aagaccgacg augacgaauu uagacaggau aaacuguucc uguacuauac acagaugggc     2460

cgcuguaugu acucuggaga gcggauugag cucagagacc uguuuaauaa gaaccuuuau     2520

gauaucgacc acauuuuccc ucgcagcaag gugaaagaug acuccaucga uaauagaguu     2580

cuggucaaga aacaaauuaa cagccauaag gacaaugacu acccccucga uucuacggug     2640

cgggaaaaga ugcgcggcca cuggcaguau cugcugagac auggguuuau cuccaagaag     2700

aaauacgacc ggcuuacccg cgugacaccc cucaccgaug acgagcuggc ggauuucauc     2760

gccagacagc uggucgaaac aagccagucu accaaggcug uggccacgcu ccugaaaacc     2820

cuuuauccua caaccgagau uguuuacgug aaggcugaac uggucucccg guuccgcaca     2880

gaguacaaau uuaucaagug cagagaggug aacgaccucc accacgccaa ggaugcguau     2940

cugaauauug uugugggaaa cgucuacaac gugcgguaua cccauagcaa agccaucuuc     3000

auuaagggcc ugcaaacgaa aaaguacucu cuuaaucgca uguuugcuuu cgacacaccg     3060

ggggccugga acaucgaaaa ugagaaaagc cuguccauug uuaagaaaac cgugaugaag     3120

aacaauaucc ucuauacaag auacagcuau augaagaaag gaggccuguu uaagauccag     3180

ccccucaaaa aggggaacgg ccaggcuccu cugaaaaagg aucuuccccu gucugacauu     3240

ggaaaguacg gcggguacga uaaaccuacc gccuccuauu ucagcuacau caaguaugaa     3300

gggaaaaagg gcaaagagug ccggcagcuc gucgccauug acaauuaccu gaagaaguau     3360

uacgaagaga accccaucga guaucugacc aauagacuug ggcugaaaaa cccggcugug     3420

cucauuccug ugaucaagua caauuccugu aucgaaauug auggauuucg caugaacauc     3480

ucuagcaaau ccaaugaggg caagacaauu gucuacaaac ccgcgguuca acuggugcuc     3540

ggggccgaaa aggaggcuua ugugaagaaa aucucuaagg uccugagcaa accugaggaa     3600

cagaagauua cguccuacga cggcgugucu gccgaggaaa acaucgagcu uuucgacgcc     3660

cugaccgcua aaaugacaaa uaccaucuuu aagauuaaac ucgaagauau cggaaacaag     3720

auuaagagcg gccgggacaa auucguuaca cuggauguga agaaacagug cuaugucauc     3780

aacgagauuc ugaagaucau gcacuguaau gugguuaccg gggaccuuuc ccugauugga     3840

gccgcgaaaa aggcuggcau cgugagcacg aacucugccc ucuccgagau caagaaaguc     3900

agcucuauua ugcugaucaa ucaauccgug acagggcucu uugaaaccaa gauugaucug     3960

cuuaacaucu ga                                                         3972


<210>  4095
<211>  3975
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4095
augagagcca agggcaaaac ccugaucggg acaagccucu uccgggaggg aaacacggcu       60

gccgaacgca gaggcuaccg gaccacacag cgcagacuga agcggcgcaa aagacggcuu      120

aagcugcucg aggcguuuuu cgaccccuau auuuuugaag uggaugagaa uuuccuggcu      180

cgccuucacg aagccgacau cuccccuaaa gaugagaaca agagauuuug ggggucucug      240

cuguucccga augagaagga cgccaacuuu uaccggaaau aucccaccau uuaccaucug      300

cgcaaggcuc ugauggaaga ggauagaaaa uucgacgucc gggccaucuu ucucgcuauu      360

caccauauca ugaaguaucg cggaaauuuc cugagcccuc uuuccguuga uucuuuuaaa      420

caagaaggcc uggagcucaa ggagcagcug gacagacagc uuaagcgggu ggccgaacug      480

uacaacgagc ucaauacaga ugugaaaauc aacgucagcg acguggaaac cauucaggau      540

aucauuaucg acaagaauaa auccagacgc gauaaggcgg gggcccugaa aaagcugcuc      600

gcuguuucua gcgaccggaa agagauuaac aagcugaaug ccucccuugc uaaggaacug      660

agcaacgcca uccucggaau gaaagcgcaa uucgccaauc ugcuucuggc ugacgaucuc      720

gagauugaca agaacaaaaa gaauuuuucu cugcuggaua caaacgccga ggaaaacgug      780

uccggccucc uggagcuugu caaugaaaaa caggcugagc uguucgacgc caucaagacc      840

auccacgauc agauuauccu caacgaaauu gugcccgacg gcaagagcau cucucagucc      900

augguugaua aauaugaggc ccauagagag caacuccagg ugcuuaaggc ucuguacgaa      960

aagauggacg cgaagcagau uaaagccauc aagaaggcuu acagccucua uaucgauggg     1020

aaagacccua aggccaaaaa ggucgauaaa cgcgcccacc agaaucgggc ugacuucuac     1080

aaggagguga agaaagugcu ggucgaacug gacgauucua gagccgagaa gcuccuggga     1140

cuuauugaaa ugggccaauu ucugcccaaa cagcgcacgg cgaugaacgg gguuaucccu     1200

aaucagcucc accaagugga gcuggacaag auuaucgaga ugcaguccaa auauuacccg     1260

uggcuggcug aaaagaaccc cgugaaaagc caucagaagc gggccccuua uaaacuugau     1320

gagcuccuga ccuucagagu ccccuacuau gugggaccua ugguuacauc caaaaagcag     1380

caacagaccu cuaauagcaa cuuugccugg auggugcgca aacaggaagg cgagauuaca     1440

cccuggaauu ucgaggaaaa ggucgacacc aucgcuuccg ccaacgauuu uaucaaacgg     1500

augacggcua aggacacaua ccuggugggg gaggauguuc ucccggccaa uagccugcuu     1560

uaccagagau auaaggugcu gaacgaacuc aauaacauua aagucaaugg acgccggcug     1620

ccuguggagc ugaagagaga agcguacguc gcccuuuucg agaacggcga cucuaaaacc     1680

guguccgcua agugcuuuaa aaacuggcuc aauguuaagc ugaagaaccu gaauagccuc     1740

aaacuggagg gccuuucuac agggaccaag uucgauaaca aucugagcac cuaucacgac     1800

cucaaaaaga ucuuuggaaa agccguggau gacgcuggcc ggcaaaagga uuucgaacag     1860

auuaucgagu ggcugacaau uuuugaagac aagaaaaucc ugcgcauuaa acuuaaagag     1920

aucgaauggc ucacggagga gaacaucacc cagcugacaa gacugaauua caacgggugg     1980

ggacggcucu ccagacgccu gcuuaccggc auuugugacu cuaaugggca gcggaucaug     2040

gauuggcugu gggacacaac caacaauuuc augcaaauuc agagccagcc cgauuuucag     2100

aaggccaucg ucgaauauaa cuccgacaaa cucgagcaag gagauauuga acagauccug     2160

ggcgaggcgu acacgagccc ucagaauaag aaagccauca gaaaggugau uaagguugug     2220

caagacgucg aaaaagcuau gggcgccaag cccacaucua ucuccauuga guucacccgc     2280

agcgauggga gaucugagcu gaccaacucc cggaugacac agcuucagaa aacccucaug     2340

agccuguccg aagagaugac agaccaggcu cugcucgccg aguucaagga uuuucaagcc     2400

ucuaaaaaga agcugacgaa uaaauauuac cuuuacuuca cccagcuggg acgcgacaug     2460

uauacaggcg aaccccucaa caucgaucag cugagccugu acuccgacgg ggaucauauu     2520

cuuccuagau cuuuuaucaa ggacgacagc cuggauaacc gggugcucac caaacgcgcu     2580

gugaauaacg agaaguccga cacaguuccg gucugcgaau auggagcgaa aaugaaggau     2640

uucuggaaug accuguacga uaacggccuc aucagcaagc agaaacuggc caaucuucug     2700

accgaccccg augagauugg gccuuauaag gcuaacggcu uuaucagacg gcaacucgug     2760

gaaacgucuc aggugauuaa acuguccgcc gagauccugc agacccuuua ccaggacaca     2820

aagauuaucg aaguccgcca aaaauauacc cacgagcuga gagagcacuu caauuuugug     2880

aagauccggc agcucaacga uuaccaucac gccuucgacg cuuaccugac agccuuuagc     2940

ggacgcuauc ucuaccagag auaucccaag cugcggggcu acuucguuua uggggauuac     3000

aauaaauuug acgcgaagaa agacagaaac uaucuuaagu cuuacaauuu ccuguacgau     3060

cucgaagagg gaaaaaccga ccaaauugug gauaacgucg aacauaaggu guuuaugaau     3120

cgccaggacg cuaucaacca gcugaagaaa gccuaugagu acaaguauau gcugguuucc     3180

caggaaacgu acacaaauag cggcgccaug uucaaagaga ccauuuaucc ugugaacccc     3240

aagcaaaaac uuauccagaa gaaaaacgau aagccggucg acauuuacgg gggauauucu     3300

aauaacuacg augcuuaccu gagcaucgug cggcucgagg acaagaaagg cauuaaguuc     3360

agaguugugg ggaucccuau gcgcaauaaa gccgaacugg auaaccucgc gaaggaggac     3420

cacaauggcu auaugcagcg gcugaacgaa aucgucgaug agcagauuaa uuccagcgga     3480

aagaagaaga cauuuagaau cauucuuccc aaagugcauu ucaagcaacu gguggaagac     3540

gauucuggca aauuuaugcu gggguccgcu gccuacccuu auaacgccaa gcagcucguc     3600

cugagcguua aauccaucga gucucuugcu aauaaggacg ccagcgaccg cgagcuggug     3660

gcuguguacg aagagauucu caacgucgug aaugauaagu ucacccugua ugacgcccgg     3720

caguuuagaa acaaacucac cuccucuaag gauaaauucg uugaacugaa uacagcggac     3780

aaggugacgg agcuucugga uauccuggac ggacuccacg ccaaccccgu cgugaaaaag     3840

cugaagauca uuagcggcuc cccuuuuggg uuccuucagc gccccucugg aaucguucug     3900

accgagaaug cuaaacucau uuaccaaagc ccgacaggca ucuuugaacg gguggucuau     3960

cugagagauc ucuga                                                      3975


<210>  4096
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4096
auggagggcc agaugaagaa caaugggaac aaucugcaac agggaaacua cuaucucggc       60

cuggacgugg ggaccagcuc cgucggaugg gccguuacag auacggacua caaugugcuu      120

aaauucagag gcaagucuau guggggagcu cggcuguuug augaagccag caccgcggag      180

gaacgcagaa cacaccgggg gaaccgcaga cggcucgcuc gcagaaaaua ucggcugcuu      240

cugcucgagc agcuguucga aaaggagauc cgcaaaauug acgauaauuu cuucgugaga      300

cugcaugagu ccaaccucug ggccgacgau aagucuaagc ccagcaaauu ucugcuuuuc      360

aaugacacca acuuuacaga uaaggacuac cugaaaaagu auccuaccau cuaccaccuc      420

cgguccgauc ugauucauaa uucuacagaa cacgacaucc gccuugucuu ccuggcccuc      480

caucaccuga uuaaauauag aggccacuuu aucuacgaua acagcgcuaa uggcgacgug      540

aagacccugg acgaggccgu uuccgauuuc gaagaguacc ucaacgagaa ugacaucgaa      600

uuuaacauug agaacaagaa agaauuuauc aaugugcuga gcgauaagca ucuuacgaaa      660

aaggagaaaa agauuucucu gaaaaagcuc uauggggaca ucaccgauuc cgaaaacauu      720

aauaucagcg uccugauuga gaugcuuucu ggauccagca ucucucuguc caaccucuuu      780

aaggacaucg aguucgaugg caaacagaau cugagccugg acuccgauau ugaagagaca      840

cucaacgacg ugguugauau ccugggggac aauauugacc uucugaucca cgcuaaggaa      900

guguacgaua uugccguccu caccucuagc cugggaaaac auaaguaucu uugcgacgcg      960

aaaguggagc uguucgaaaa gaacaagaaa gaucucauga uccugaagaa auacaucaag     1020

aaaaaucacc cggaggacua uaagaagauc uuuuccagcc ccacagagaa aaagaacuac     1080

gccgcuuauu cucaaaccaa uuccaaaaac guguguagcc aggaagaguu cugccuguuu     1140

auuaagccuu acauucggga uauggucaaa ucugaaaaug aggacgaagu ucgcaucgcc     1200

aaggaggugg aggauaagag cuuccucacg aaacugaagg gcacaaacaa uuccgugguc     1260

cccuaccaga uucacgaaag agagcuuaac cagauccuga aaaacaucgu ggcuuaucuc     1320

ccuuuuauga augacgaaca agaggauauu ucuguugugg acaagaucaa acugauuuuc     1380

aaguuuaaaa uccccuacua ugucgggccu cugaacacca agagcacacg guccugggug     1440

uacagaagcg augagaagau uuauccgugg aauuucucua acguuaucga ccuugauaaa     1500

accgcccaug aauuuaugaa ucgccucauu ggacggugua ccuacacaaa cgaccccgug     1560

cugccuaugg acucccugcu cuauagcaag uacaaugucc ugaacgagau caaucccauc     1620

aaagugaacg gcaaggcgau uccugucgaa gugaaacagg ccaucuacac ggaucuuuuc     1680

gaaaauucua agaagaaagu uaccagaaag uccauuuaua ucuaccugcu caaaaacggc     1740

uauauugaaa aggaggacau cgugagcggg aucgauauug agaucaaauc caagcugaag     1800

ucucaccaug acuuuacaca gauuguccag gaaaauaaau gcacccccga ggaaaucgag     1860

cgcauuauca agggaauccu gguguacagc gaugacaaau ccaugcuucg gagauggcuc     1920

aagaacaaua uuaaaggccu gucugaaaac gauguuaagu aucuggcuaa gcucaacuac     1980

aaagaguggg ggcgccugag caagacacuu cugaccgaca ucuauacgau uaauccggag     2040

gauggagaag ccuguuccau ccucgacauu auguggaaca caaaugcuac ccugauggag     2100

auccugagca acgaaaaaua ccaauucaag cagaauaucg agaacuacaa agccgaaaau     2160

uaugaugaga agcagaaccu ucacgaggaa cucgacgaua uguacauuuc uccugccgcu     2220

cggagaucca ucuggcaagc gcugcgcauu guggacgaga ucgucgacau uaaaaagagc     2280

gcccccaaga aaaucuuuau ugaaauggcu cgggagaaga aaucugccau gaagaaaaag     2340

agaaccgagu cccgcaagga uacacugcuc gaacuguaua aaagcugcaa gucucaggcc     2400

gacggcuucu acgaugagga acuuuucgag aaacugagca augaauccaa cagccggcuc     2460

agacgggacc agcuguaucu guacuauacc cagauggggc gcucuaugua cacaggaaag     2520

agaaucgauu uugacaaacu uaucaaugau aagaacacgu acgacauuga ucauaucuau     2580

ccucggucca agauuaaaga cgauagcauc accaauagag ugcucgugga gaaggacauu     2640

aacggcgaga aaacagacau cuaccccauc uccgaagaua uucgccaaaa gaugcagccc     2700

uucuggaaaa uccugaagga gaagggccug auuaaugaag agaaauauaa gcggcucacc     2760

agaaacuacg aacugacaga cgaggagcuu ucuagcuuug uugcucgcca gcuggucgaa     2820

acccagcaau ccacgaaagc ccucgcgacc cugcugaaga aagaguaucc uucugcuaag     2880

aucguguaca gcaaggccgg gaacgugucc gaguucagaa aucggaaaga uaaggagcuu     2940

ccgaaauuuc gcgaaauuaa cgaccugcac cacgccaagg augcuuaucu caauaucguc     3000

gugggaaacg uuuacgacac aaaauucacc gagaaguuuu ucaauaacau cagaaaugag     3060

aacuacucuc ugaaacgggu guuugauuuc agcguccccg gcgccuggga cgcuaagggg     3120

uccacauuua auaccauuaa gaaauauaug gccaagaaca auccuaucau ugcguucgcc     3180

cccuacgaag ugaaaggaga gcucuuugau cagcagaucg uuccuaaggg caaagggcag     3240

uuccccauua agcaaggcaa ggacaucgaa aaauauggag gcuacaacaa gcugagcucu     3300

gcuuuccuuu uugccgugga guauaaaggg aagaaagcuc gcgagagauc ccuggaaacg     3360

gucuacauua aggaugugga gcucuaucug caggacccga ucaaguacug ugaaagcguu     3420

cugggacuua aagagccuca gaucauuaag cccaaaaucc ugaugggcuc ucucuucucc     3480

auuaauaaca agaaacuggu ggucacaggg cggagcggaa agcaguacgu gugccaucac     3540

aucuaucaac ucucuauuaa ugaugaagac ucccaguacc ugaagaacau cgccaaauau     3600

cuucaggagg agccugacgg caauaucgaa cgccaaaaca uucugaacau caccagcgug     3660

aauaacauua agcucuuuga uguccugugu acaaaauuca auuccaacac cuacgagauc     3720

auucugaaua gccuuaagaa cgacguuaau gaagggagag agaaauuuuc ugaacuggau     3780

auccucgagc agugcaacau ccugcuccag cugcuuaagg cguucaaaug uaaucgggag     3840

agcuccaacc uggaaaagcu gaauaacaag aaacaggccg gcgugauugu gaucccccau     3900

cucuuuacca agugcucugu cuucaaagug auucaccaaa gcaucacagg acuguuugag     3960

aaggaaaugg accuucugaa auga                                            3984


<210>  4097
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4097
augaagaacu acagcaucgg ccuggacauu gggaccggau ccgugggcug ggucugcauc       60

gaugagaauu aucagauucu cagauacaac aaucgggccg cuuucggggu ucacgaauuu      120

gagacagccc auguggcgga agaacgcaga aucaagcggg gagcucgcag acgguauaac      180

cgcagaaaga aacggauuca acugcuucag ucucuguucg ugccccacau gcagcagaag      240

gccuuuuucg ccccuacgau caccgaacau uacuggaaaa augacaacca auuugagaau      300

cgcacacuca gcgagguccu gaugucccuu ggcaugaacc cgaauaccua ucccacaauc      360

uuccaccuga gacaugaacu cauugagucu agcgugaaga aggaugcucg gcugaucuac      420

cuggcccucc acaaccuggu uaaauuucgc gggcacuucc uucaggaagg auccaccugg      480

aaugcuaaca ugucugacgc caaucugagc gagcaguuug agcagcuccu gcauauuuau      540

acagaaaugg aggaacuucc ucaagugucc cugaccgcgc agcagagcac ggaugucgag      600

cacauccucc ugaacaauca uauuucuaag gccgaaagag cucggcagcu gaccgcccuc      660

uucgagcgca cauacgagcc cuuuuucaaa cugaucgugg gccuugacau ugcugccgcg      720

caacuguuua agcaguccga acagcaacuc cuguacaaag agagcaagac caagguuaac      780

cuuacagccg aaacccugcc ugaggugcuc cagacgaugc uggaacccga ggucgcuuuc      840

aucgaggccg ugcagccuuu cuaucagcaa acagugcugu uugaucuccu gaaaggccag      900

ucuuacgucg cugccgccaa gguugaggcu uucaaccagu augagagaga ccuugcgcug      960

cucaaauccg ugaucaauga aguggggagc gagaagcagu accgggccuu uuucauuacc     1020

ucuaaacaaa agaaaagaca guuugaacag aagccgaaca uggagcugcu uugucaccug     1080

gaucgcuuca aucgggagaa gaaauaugaa gagacauuuu acaaggaagu cuccaaacuc     1140

cugaccgcuc ugaucaagga ggccaccgga gaggugaaag aacacauuga gcagaucauu     1200

caagccaugg aaagccaguc cuuccucguu aagcagaagg gcauccagaa cgcuucuauc     1260

cccuaucaaa auagccugua cgaggccgaa augauucuua gaaaccagca ggcguacuau     1320

ccuuggauca cagacgaguu uauugagaaa gugaagcaac ugaucgcuuu ccgcauuccc     1380

uacuauaucg ggccucucgu ccagggagau gaacagcccu uuggcuggcu gcagcggcaa     1440

gccgacggga auaucacgcc guggaacuuc gaugagaaag ugaauaaggc cgcuuccgcc     1500

gagagcuuua uuaccagaau gacaaagaac ugcaccuacc ugaaggagga aaagguucuu     1560

ccuaaacagu cucucaugua ugagaaguuc acacugcuga augagcucaa cuccgugcag     1620

auccgcagcu cucuggaaca gccccauaaa aagcaccggc uuugugagga caugaaacaa     1680

uggauuaucg aacaugucuu caagaagacc aaaacgguga cacacaagac ccugauucag     1740

gcucucaaaa agagcccuua caaagacgag cuguaugaug aauccaccgg aaauuggaag     1800

gacgucuacg gcacacagaa agaggauaga uuugccucua gccuguccac cuacaucagc     1860

cuuaaggaga uugugggcga caucguugau aacgcguuca uggaagagau cauuuauugg     1920

cucacagugu uuacggaccg cgauaucauu acccugaaga ugaaugacaa auaccccuuc     1980

aucacagaac agcaaauuca gcggcugcuc ucucugaaca ucuccgggug gggacagcuu     2040

agccaaagac ugcuccaguc uauccccguc gaugacauga accgcuccgu gauugaguau     2100

auggagaagc augccguuaa uuuuauggaa cugcugagcc cucggcaguc cgaucuugcu     2160

uacaaaaucg aacaggugaa caagcaacac ucuaaaaaga uucacaagau cagauaugaa     2220

gacauugagc agcucaccgg cagcccggcc cugaaacggg ggaucuggcg cuccgucaag     2280

aucauugagg aacugacagc uaucuucgga gagcccaaaa auauuaugau cgaaguggcc     2340

agagaggacc aggcgucuca ucggaccaag agcagaaaag aacaguggga guccauuggc     2400

aagagccucg ccaaggaaga gaaagagcug aaggccuuuu ucaaagaaau ggcucaauac     2460

ccuaagcagc aguuugaucg cacgcgguuc uggcuuuaug ugacccagca agggaaaugc     2520

cuguacacag gaaagacccu cagacuggag gaccugucuu ccuacgaaau cgaucacauc     2580

cuuccccgca acuuuguuaa ggacgauagc auugacaauc ucgcucuggu cuauccuaug     2640

gccaaccagg agaaaaauaa ggccggcaac aauaaaaugc cccuggaaau cauugaugcu     2700

aagcagcagc cggcgaugau ggcccauugg aaaagacuca accaacugaa acuuaucucu     2760

gacuccaaac ugcacaagcu caugaagccu agcuucaaug auguggacaa agagaacuuu     2820

auugcucggc agcuggugga gacacgccag aucauuaagc acgucagaga ucugcuucaa     2880

gaacgguucg agcauaccga cauccacgug guuaaagcca agaugguguc ucaguuccgc     2940

aaauuuagca gaauccccaa gauucgggau uacaauaaca agcaucacgc cauggacgcu     3000

cuguucacga caguccucau ccaguuuauu cugcagaaau auggcaagaa uuuccuccaa     3060

uuugaccugc agcauaaaga uaucuccaag aaauggagca agauugccca gucuaccaag     3120

cacuucuuuc uuuuccagca auuugcggaa cagcagauca aauccccugu gacaaaggag     3180

cugguuagcg gggugcagua cuucgagcac aucuuuuaug aacaauccug gcagaccacc     3240

aaaaugacac agacgauuga cucuaacuuc uacaaggaga ccaucuucag ccccaaacuc     3300

aagacagcua aguauaccuc cgccaaagcc gaaaaguuug uccaugaugc uauugugaaa     3360

uacgccguuu guggaaucca auacacaucu cugaagaaaa acaaggagca gcaccaugug     3420

cugcuuaccg acauuaaugu caucgaacac uaucagcuga aggaggcgag ccucaacgag     3480

cuggcucucc aucuggccaa acgcgaaucc aaggaugacg ugaucgaugc ccagaugcuu     3540

guggucauuc cuaaauacaa gaaaaucaug cugaauggcu cucccuauua cugcauuagc     3600

uccgacgaga gacacaucgc uacgcaauuc guucuccgga aggaucugcu ggaagccgug     3660

cuucacguga acgacgagac aagcgucgau gaacugcgca cccucuuuag aucuauugug     3720

gaggagauga ugacccagua uaaaguuuac aauaaggaca caauccggaa caagauugau     3780

gcuuucaucg aaaccgagcu gaucgacgug gaaacauuuc aggucggguu cacggagauu     3840

aaaaagaugg uggccgcgaa ugcccagaga uccgacaaau uuggaggccg ccuccggcaa     3900

guuaugaagg uggaagauau cuauauuauc gacgagagca uuaccgggcu gagauaccgc     3960

aaaccgcgga aacuugucaa guga                                            3984


<210>  4098
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4098
augaagaaaa acuacaccau cggccuggac auugggacag ccagcguggg augggcuguc       60

cucacggagg auuaugaacu gaucaagaga aaaaugaaga uuuccggcaa uacccagaaa      120

aaggccguua agaaaaacuu cuggggagug cggcuuuuug agcaaggaga aacagcggag      180

ggccgcagac ugaagcggac cacacgcaga cggaucgcuc gcagacggca gcgcauucag      240

uaccucagaa ccaucuucga cgaagggaug aaucaggugg augccaacuu uuucgcccgg      300

cuggacgagu cuuuuagcau cauugaugag aaagaaaaug agcgccaccc caucuucgga      360

aacgucgcug aagaggccgc uuaucaugag caauuuccua caauuuacca ccuuagagaa      420

caucuggcca auuccucuga gcaggcggac cuccggcugg uguaucuggc ccucgcucac      480

aucauuaagu uccgcggcca uuuucugauc gaaggcgagc uuaacaccga aaauagcucc      540

guuucuggga cguucgagca guuuauuaaa guguacaacg agaccuucaa ugucgaaaag      600

gcccuggauc ucacagugga ccuggaugga aucgcugagc agaagaucag cagaaugaaa      660

cgggccgaac uuauucuguc ccucuuuccg gaggaaaaga gcaccggcga cuucgcgcaa      720

uucaucaaaa ugauuguugg gaaccaggga aaugugaaga aaacauuuuc ucugaacgag      780

gaugccaaga uccaguucuc caaagaggaa uaugaggaaa aucuggagac ccuccuggcu      840

gaaauuggcg aggacuuuag aagcgucuuc gaugccgcua agucugugua cgacgccauc      900

ucccuugcca acauccugaa ggugacggac gcuacaaccc gcgcgaaacu cucuagcucc      960

auggucguuc gguuuacaga ucacaaggag gaccugaaaa cccuuaagag auucgugcgc     1020

gaaaaccugc ccgaugagua cgacgaucuc uuuaaaaaua agaagguggc cggguaugcu     1080

ggauacauug acggcaacga aacccaggag gccuucuaua aauaccugaa gaaaacacug     1140

gccaaggcua cgggcgccga gggguuucuc gcgaaaaugg aacaagagga uuuccugcgg     1200

aagcagagaa ccuuugacaa uggagucauc ccuuaucagc uucaccugga ggaacucaag     1260

gcuauuauca aaaaccaaaa gagcuacuau uccuuccugg augaagagaa aauuucucag     1320

cuuaugacau uucgcauccc cuacuacgug ggcccucugg ccaaggagaa agggcaguuc     1380

gccuggcuca cccggaagga aaugggaaag aucacacccu ggaaucugaa cgagaaaguu     1440

gacauugaaa agagcgcuac cgauuucgug gagagaauga cgaauaacga cuccuaucug     1500

ccuauggaaa aaguccuccc gaagcauagc cugcuuuacg agacauuuac cguguauaau     1560

gagcugacca aaguucgcua caucgacgau aacggccggg cccagaauuu cucuuccaag     1620

gagaaaagac aaauuaucaa cgaacucuuu aagcagcagc gcaaggugaa aaaggaaaug     1680

cuggaggcuu uccugaaaaa ugaauauggg auugagaacc ccaaggucga gggaaucgaa     1740

aagagcuuua augccugccu uggcacauac cacgaccuca uuaaacuggg cauccggccu     1800

gagcuguucg aacagcccga gcaugagcaa caguuugaac agaucgugaa gauucucacc     1860

gucuucgagg auagaaaaau gcggcgcaag cagcuggaaa aauuuucuaa cauccuuaca     1920

gaggaggaac aaaagcagcu ggagagaaaa cacuauaagg gguggggacg gcucagcgcg     1980

aagcugauuc acggcaucgu ggaccagaaa acgcagaaga ccauucugga uuaccuuauc     2040

gacgaugacg aucucccuaa aaacagaaau cgcaacuuca ugcaacugau caaugacgaa     2100

aaccuguccu uuaaggagga aauugagaaa aucgccuucg auaaugacaa gucuacagaa     2160

gagauuguuc aggagcucgc ugggagcccc gccaucaaga aagggauucu gcagucccuu     2220

aagaucgugg aagagaucau ugauaucaug ggcgaacugc cgaccaacau ugucguggag     2280

auggcucggg aaaaucaaac aaccgcccag gggaacagag cgagcaaagc ccgcaugaag     2340

uaccucgagg agucuaucaa aaagcugggc uccagcauuc uggaagacga gccuaucucu     2400

aaagacgcua aucuucucag aaacgaucgg cuguuucugu auuaccucca gaauggacgc     2460

gacauguaua cgggcaacga acuggauauc aauaaccuuu ccagcuacga cauugaucau     2520

aucguucccc aguccuucgu gaaggacgau ucuauugaca auagaguccu gaccacaagc     2580

uccaugaacc gggggaaguc uaacaccgug ccugccgaga gcgugguuaa aaagaugcgc     2640

cccacauggg agagacuccu ggcuuccgga cugaucagca aaaagaaauu cucuuaucuu     2700

accaaggcca cgaauggcgg gcucacagaa gaggauaagg cccacuuuau ucaacggcag     2760

cuggucgaaa cccgccagau caccaaaaac guggcucaga uucugcauca aaaguacaau     2820

aacgagcagc ucuccgaaaa acccgugaga gucaucacac uuaagagcgc gcuggccucu     2880

caguuccgga aagacuuuuc ccuguauaag auccgcgagc ucaaugauua ccaccaugcu     2940

caggacgccu accugaacgg agugauugcc caagcucugc uuaagguuua uccuaaacug     3000

gagccggagu ucguguacgg cgaauaucag aaggucagca ucagagcccu caauaaagcg     3060

accgcuaaga aagaauuuua cucuaacauu augaauuucu uuacaagcga ugagauggug     3120

gccaacgaag agacggggga gguucugugg aaucggcaga gagacaucaa gaccauuaag     3180

aaagugauga acuaucacca aaugaauauc gucaagaaag uggaaaucca gacaggcgcc     3240

uucaccaagg aguccauucu ccccaaagga ccuagcaaga agcugaucgc ucgcaaaaac     3300

aauugggccc ccguuaacua cggcggggug gacucuccua cagucgcgua uuccgugauu     3360

aucacccacg aaaagggaaa agcugccaag gugguccagc agcuuguugg cauuaaaauc     3420

cuggagcggc aagccuuuga acaggaugag guggcuuucc ucgaggaaaa gggguuuauc     3480

caucccaaag ugcagcugaa gcugccgaag uacagccuuu accaguucgc cgacggaaga     3540

cgccggcugc ucgcuuccgc cgaggaaucu caaaaaggca accagauggu ccugccugug     3600

caccucauug agcugcuuua ucaugcgaag cacguuagcg auuccucugg gaaaagccug     3660

gaguaccuca augaacacag acaugaguuc gccgaacugc uggaggcuau ccuucaguuu     3720

acggaacagu auauugacgc cggcaagaac cagaaaaagg ugcgcgaucu guacgagaag     3780

aaucaagacg cugauaugcg ggagcucgcc uccucuuuca uccagcugcu ccagcugaac     3840

aaacaaggag cccccgcuga cuuuaaguuc uuuggcgaaa caauuccuag acgccgguau     3900

aaaaauaccg cggagaucgu cgaugccacc uucauuaacc agagcaucac agggcuuuac     3960

gaaacccaga agagacuggu guga                                            3984


<210>  4099
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4099
augaccgagc agcaacagcc cuacaacauc ggccuggaca uugggacaag cuccaucgga       60

ugggccguga cggaucagaa ugacaagcuc cugcacauua gaggccauaa cgggaucgga      120

guccggcuuu ucaaagaagg cgagucugcu gccgaacgca gaggguuucg gaccacacgc      180

agacggcuga gccgcagacg guggcgccuc agauuccugg aucggauuuu ugacgcggag      240

cuugcuaagg uugauccuaa uuuccuggcc cgccucaaac aguccaacau cucuccgcug      300

gaccccaaua agcaaauguu uggaaacauc cuguucgaug acccucaguu ugaugaccag      360

aaauuccacc agcaauaucc caccauuuac caucucagaa gcgcccuggc ucagccugau      420

gcuccccagg ccgacgugcg gcuuaucuau cuggcccucc accauaugau uaaguuucgc      480

ggccacuucc ugaauggcgc ggccguggaa aaguuucacg ggggacagcu ucaucuggag      540

uccgaguucc aagaacucaa ccagaucuuu ucuaaucagc aaagagauca cguccagcug      600

cagacaagcu cccuggacga gguggcuacc auucucaaag acaaccagcg gacaccuagc      660

gauagacaac gcgaacuguc ugcccggcuu uucguuccgg uggacaagac caucgauaaa      720

gagaauaaga aacuggcuac ggagcuccug aaggccgucc uuggccugaa ggcgaaauuc      780

gacgugauuc ucgggguuga agccaccgau ccccaggcuu uuaagcugca guucuccagc      840

gacgauuuug acgauaaaau cgccgagcug gcuucugaac ucacagacga ggccaacgaa      900

auccugaccg ugcuucagag acuguacuuc ucccucaauc uggccgauau ucuuacaaac      960

cgccaaacca agcaggucgc ugacagcauc ucuacggcga ugauuaaucg guaugacgau     1020

cauaaacacc accugaagau gcucaaagag cuggaggccc aggugacaca ggaacaaaga     1080

gacggacuga agaaggcuua caacgaguau aucgauggcg acaaaccuac cauugauaca     1140

uuuuacacca acgugaagaa aaaucucgac gauuccgaac uggccaacca gaucaagagc     1200

cuuaucgacc ugcaggccuu caugcccaaa cagcgcucca aggagaaugg gucuauuccu     1260

caucaacucc accagcggga acuggaucag cugaucgagg uccaaggaaa guacuauccc     1320

uggcuucucg cugccaaaga caagcuggau gcgcugauug cuuuuagagu uccguacuau     1380

gugggcccua ugaucgaccc caaccagagc gaugacccuc agaauaccaa auucgccugg     1440

auggugcgca aggaggccgg cgaaauuaca cccuggaacu uugaggaaaa agucgacaag     1500

acguccagcg cuaccaauuu caucaagcgg augacaacca cagauaccua ccuccugggg     1560

gagcccgugc uuccuaaaaa cucucugcuc uaucagcaau uuacgguucu gaaugaacug     1620

aacaaacuua aggugaaugg aaaggcccuc acagucgacc agaaaaagua ccuguaugau     1680

caugugugca agaaacagag acgcguuacc cugaagaaac ucuccgacug ucugguggcu     1740

cggggcgauu ucccgauggg ggccaucuuu accggaacaa ccgaccagaa gaacaugaau     1800

aacacacuua aaacguacca agauuuccag gagauuuucg gcgacgagau cgauaauccc     1860

gacagacagg ucgauuuuga aaagaugauu gaguggagca ccaucuucga agaccgggag     1920

auuuuuaaac ugaagcucgc ggagcugaac uggcugacau cugaacagcu ugagcgcgug     1980

agcaagaaaa gauaccaagg guggggacgg cucucccaga agcugcugac ccacaucguc     2040

aacgaaaaug gcgagagaau uaucgaucag cucuggaaca caaaucaaaa cuucauggaa     2100

aucaugggcc aggacucugu gaaacaggcc auucaggagc auaauggggc uaacguugcc     2160

caaaccggaa ucaaugagac gauugacgaa cuguauacca gcccucagaa caagaaagcu     2220

auccgccaga uucuucuggu ggucgaugac aucaagaagg ccgugggcua cgcgcccgcc     2280

aauaucauga uggaguuugc ucgggaagau agagacgauc accgccucau ugccuccaga     2340

gcucggcagc uggagaaaac auaucaagaa aucagcaaga acugguucga caaugagucu     2400

guuacccagg agcugaaaga ucagauuaag aacaaaacaa aguuuaccga ccgccuuuac     2460

cucuauuuca uccagggagg aauugaucug uacacgggcc acccucugaa uaucgaccaa     2520

cucuccagcu augauaucga ccauauucug ccccagucuu uucuucugga caacucccuc     2580

aauaacagag ugcugacaag ccaggccgaa aaccgggucc acaaggagga ucgccugccg     2640

ggggaaaccg uggccaauaa aaugagaccu acauggaaga ugaugcggga gcagggccuu     2700

augacccaac gcaaauacaa caaucugacc cucaagcccg accagaucuc caaauucaac     2760

aauagaggac aguuuauuaa ccggcaacug guggaaacac gccagguuau caagcucgcu     2820

gcggauauua ugucucagaa guaccaggcc gaccaaacga aagucgugac cgugaaggcu     2880

aaucugacac aucacaugag agagaaauuc aacuuuuaua agagccggaa ugucaacgau     2940

uaccaccaug ccuucgacgc cuaucuuacc cuguucgugg gcaauuggcu ccugaaagag     3000

uacccuaagc ugcagcccua uuuuguuuac ggggauuucg cuaaaacagg aaccaacaau     3060

cuuaaguccu uuaacuuccu guauagauuu gaacgcgacc acaagcucaa ugauaaaggc     3120

gccaucgaca cggauaagga ccagcagcug ggguacauga aacggaucua cagauucaag     3180

aaaaugcucg ugacaaagca acuggagacc aacgaaggag cgcuuuaugg ccagacccug     3240

uacccugcuc ccaagaacga uaaaaagaca cgcaaucuca uuaacucuaa aaagaaucgg     3300

ccgagcgaca ucuauggggg cuacuccaac gagaauggga gcuuucugac ccuggucaaa     3360

cuuaagaagg gcucuaaaua ugacuacaga cugguggccg uuccuguguc caagauuagc     3420

gagaucaaag aaucugagcu cgccacaauu gaaaaggcua ucgcgcccca guuccugaaa     3480

uccagcggga aguaucgcga uuuucagguc gugcucucua agauucugau caaccaaaaa     3540

guuaucgacg auggaaugau uuucacgauc ggcagcaagu ccuacaaaca uaaugccaaa     3600

cagguggucc uuagcgacaa cucuauucag aucgugucca cccugagccg gauuuccgau     3660

aaggagaagc ugaucacagu guuugacgaa aucgucgaua uuaccucuaa guaccucccc     3720

ugguauaaag uuaaugacgu ggagaagaca aaaaugcugu ucagacaaau gcaggauauc     3780

gacgagauga agaaaauuau cgauaacagc cuuauugggc ugcacugcaa ugcuuccauc     3840

accgaccucg gaaagguggg ccugucuaaa uacugggggc gcuucguccu cacggccggc     3900

aucagccuga ccguggaugc ccagcuuauu uaucaguccc cuacaggacu guuugaacau     3960

cggguuaagg ugucugaccu guga                                            3984


<210>  4100
<211>  3990
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4100
augaacaagc agcccuacaa uaucggccug gacauuggga ccagcuccgu gggauggucu       60

gccacagagc ucgauaacag acugcuucac aucaaaggcc auaaugggau uggaguccgg      120

cuguucaagg aaggcgagag cgcugccgac cgcagagggu uucggacgac ccgcagacgg      180

cucgcgcgca gaaaauggcg gcugcgccuu cugaacgaaa ucuucgcuac agagauugcc      240

aagguugauc cuuccuuuuu cgccagacuc aaacaaucua augugagccc gaaggacccc      300

aacaagacca uguuuggaaa uauccuguuc gaugacgaaa accuggauga ccagaaauuu      360

caccaugauu auaagacaau cuaccaccuc cggcaggcua uuaucaauca uccugagcag      420

aaauucgaca uucgccugau cuaucuugcc augcaccaca uuaucaagua cagaggccau      480

uuucugaacc aagcuaaugu gcaggauuuc aaaggcggga agauugaccu cgagaagucc      540

uuuaaagccc ugaacgauau cuucgaaaau cagggacggg accuucgccu ggucaccgac      600

gaggcgaacc aguaugugaa gguucucucu gauaaugcca aaacaagaag cgaccggaag      660

aaagaacugu ccaagcugcu cuacgugccc aucgauaaag acaccgauaa gaacagcaag      720

aaagucacgg gcgagauucu gaaggcuauc cuugggaaca aagccaaguu ugacgugauu      780

uucggaaucg agguugaaaa uccuaaagag ugggugcuga ccuucaacuc ugaugacuuu      840

gaugacaaga uugaaggccu cuccagccaa augacagaug aggacucuga aauccugcuu      900

auccugaagg agcucuauuu cgcucugaau cuguccgaca uucucaacaa uagcgucacc      960

gauaaaaacg ccaagacacu gucugaggcg augaucaaua gauacgacga ucacaaaaac     1020

cagcuugcca ugcugaagaa agugauugac uccagcgaua agaagcaugc uaaagaacuc     1080

aaggaggccu acuccgcuua uaucgacggg gaaaauaaca aaaagauuuc uuuugaggau     1140

cugaaaaagc gcauccagaa gaaucuugug gacagcgaac ugucccagaa aaucaacgau     1200

cuccuggacc acgauuacuu ccugcccaag caacggagca aagagaaugg agccauuccu     1260

caccagcucc agcaacagga gcuggaccag aucauugaau cucagaagca auauuacccg     1320

uuucuugccg agcugaaccc caaugaaaag agaucccgcc aggcuaagua uaaacucgau     1380

gagcuggucg cguuccgggu uagcuacuau gugggcccua ugguggaagu caccgacucu     1440

aaccaggaga acaauggcaa guuugccugg augaucagaa aggagcaggg ggaaauuacg     1500

cccuggaacu ucgacgagaa aguggaucgc acagaaaccg cuacaaaguu uaucacccgg     1560

augaccacaa cggacaccua ccugauugga gagccuguuc uucccaaaaa uagccuccug     1620

uaccaaaagu ucgugguccu gaacgagcuc aauaaccuga aaaucaauca ggaaacaauc     1680

accccgggcc agaagcagua ucuuuacgag caugugauga agaaaaacaa gagaguuaca     1740

cugaaaaaga uugccgaugc ccucgugauc aaaggggacu auccuuacgc ucccgaaguc     1800

aagggaacca cggaucaaaa gaaucugaac aauggccuga caaccuauau ugaccuugcc     1860

aaaaucuuug ggaacgagcu cgaugacgug gaucgcgaag cggacuucga gaagauuauc     1920

gaguggucca ccaucuuuga agaucagcac auuuucgucc ugaaacugca ggagcucaag     1980

uggcugacag aaaaacagcg gaaucaagug guuaaacuua gauaccaggg auggggcaag     2040

cugucuaaag agcuccuggc uggccuggug gacgauaacg ggcagcgcau cauugaccuu     2100

cucuggagcg acaaucaaaa cuucaugcag aucgucaacc agaaguccuu uaaagaaagc     2160

auugccgauc auaauggagc ccaccuggag gagaagaacc ugaaagacgu gaucaaugau     2220

cucuauaccu cuccucagaa caagaaagcu auccggcaag uuauucuggu ggucgacgau     2280

aucauuaaug ccgugggcua cgaacccgcu aacaucauga uggaguucgc cagagaagac     2340

uccaaugauc aucggcuuac aaacagccgc ucuagacagc uggagaaugu guacaaggac     2400

auuacgaacu ccugguuuga uaaugacagc guuaaggaug aacucgagga caaaaucaag     2460

aacaaagaca aguucaccga ucggcuguau cuguacuuua cacaaggggg aaaagaccuu     2520

uauaccggcg agccccucaa uaucgaugac cuguccucuu acgauauuga ccacauccug     2580

ccucagagcu ucauucucga uaacucccug gacaauagag uccuuacauc ucagcgcaac     2640

aaccggguga gcaaggaaga ucagcugccg ggggagcucu uuggcaccaa gaugcacgcg     2700

uucuggaaaa gacugaaggc cgcucaucug aucuccaaua aaaaguauua caaccuuacg     2760

cugaccccca auagcauuuc uaaauuuaac aaucagggac gcuucaucaa cagacagcuc     2820

guggaaacac ggcaagucau uaagcuggcc gcugagaucc uccacaaucg cuauuccgag     2880

gaaaagggca ccaacaucgu gacaauuaaa gccaaucuga cccaucacau gagagagaag     2940

uuuaacuucu acaaaaaucg gaacguuaau gacuaccacc augcguuuga ugccuaucuu     3000

acggcuuucg uggggaacug gcugcuccag caguacccua agcugaaacc cuauuucguc     3060

uacggagacu uugccaagac agaaaucaau aaccucaaga gcuucaauau gcuuuauaaa     3120

uuugagcaag cucaccagcu gaacgaaaag gauggcaaaa uugccaagga caccgacgcc     3180

cuccuggggu acaugaaaaa cguguaucau uucaagaaga ugcucguuac aaaagagcug     3240

gagaccaauc acggagcucu uuuuaaccag acccuguacc cuucucccgc gcaugauauc     3300

aagaaacgcc agcucauuaa gccgaaagac aauagaccua cagauaucua cggcggguau     3360

acgcacgagg aacggaagua caugucccug gugaaaguca ucggcaagaa ggacaacgaa     3420

uaucgcaugg ccaaugugcc ccugcuuaaa auuaacaaga auaacgauau caaagagauu     3480

cucaagagcc aauucucuaa uaaaaaguuu gaaaucguuc ugccuaaggu gcugaaaaac     3540

cagcuguuca gcgagaaugg agcucuguuu gucauuggcu ccaaggggua caaauauaac     3600

gccaaucagc uugugcugag cgaccuggau augaagcucc ugaacgaccu uucuaauaac     3660

aaauccaucg augaggugac caucaaccag cuguucaaga auauugucga cagcacaaac     3720

aaguacaugc cccucuauga aaucaaaaau cugaacgagg uuguggaaaa guuuaauaac     3780

cucgaguuca augauaaaaa gaaaaccauu acagacuucc ugcuugccac ccacgcuaac     3840

ccuacgaugu ccaaucugaa gaacaucgga cugcccuacu uuggcagauu cauucggaau     3900

acagggauca agcucucuga aaacgccaau gugauuuacc aaagcccgac cggacuguuu     3960

ucccgcaaaa ucaagaucuc ugauuucuga                                      3990


<210>  4101
<211>  3993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4101
augaagaacg acuacaccau cggccuggau auugggacaa auagcguggg auauuccguc       60

guuacggacg auuacaaagu gaucucuaag aaaaugaacg uguucggcaa uaccgagaag      120

aaaagcauua agaagaacuu uuggggaguc agacucuucg aauccggaca gacagcccaa      180

gaggcucgga ugaaacgcac cucuagacgg cgcaucgcca gacggaagaa ucgcauuugc      240

uaucugcagg aaaucuuuca gcccgagaug aaccaccuug acaauaacuu cuuuuacaga      300

cugaaugaau ccuuccucgu ggcggaugac gcuaaauaug auaagcaucc uaucuuuggc      360

acacuggacg aggagauuca cuuccaugaa caguuuccga ccaucuacca ccuucggaaa      420

uaucuggccg auggggacga gaaggccgau cuccgccugg uuuaccuggc uauugcccau      480

aucauuaagu ucagaggaaa cuuucucauc gaaggcgagc ugaauacaga gaacaauucc      540

gugauugaac uuucuaaagu cuucgugcaa cuguacaacc agacccucag cgagcuggaa      600

ggcuuucagu ucaucgacga guccaucgau uucagcgaag uucuuacgca gcaacugucu      660

aaguccgagc gggcugacaa ugugcucaaa cuguuucccg acgagaaggg gaccggaauu      720

uucgcccagu uuaucaaacu gauugucggc aaccagggga acuucaagaa aguguuucag      780

cucgaagagg aucaaaagcu gcagcuuagc acagacgauu augaagagaa uaucgaaaac      840

cugcucgcga uuaucggaga cgaguacggc gauaucuucg uggccgcuca gaaucuguau      900

caagccauuc uucuggcugg gauccucacc ucuacagaga agacccgcgc caaacugucc      960

gcgagcauga uucagagaua cgaagaacac gccaaggacc ugaaacuccu gaagcgguuu     1020

gucaaagaac acaucccuga uaaguaugcu gagauuuuca acgacgccac gaagaaugga     1080

uacgcuggcu auaucgaugg caaaacaaag gaagaggagu uuuacaaaua ccuuaagacc     1140

acacugguuc agaaaucugg guaucaguac uucaucgaaa agauugagca agaaaacuuu     1200

cuccgcaagc agagaaucua ugacaaugga gugauucccc aucaggugca cgccgaggag     1260

cugcgggcca uccuuagaaa acaggaaaag uacuauuccu uccugaaaga gaaccaugaa     1320

aagauugagc aaaucuuuaa aguccgcauu ccuuacuaug ugggcccccu cgcuaagcac     1380

aaugaacaga gccgguucgc guggaacauc agaaaguccg augagccuau ccgcccgugg     1440

aauaugaacg acguugugga cgagaaugcc ucugcugucg ccuucauuga acggaugacc     1500

aucaaagaua uuuaccugaa cgagaaugug cugcccagac acagccucau cuacgaaaag     1560

uuuaccguuu ucaacgagcu gacaaaagug cuuuaugccg acgaucgcgg ggucuuucag     1620

cgguucuccg cugaagagaa ggaggacauu uuugaaaaac uguucaagag cgagagaaaa     1680

gugacgaaga agaaacucga aaacuaccug cgcaucgagc ugucuaucuc cagcccuucu     1740

gucaagggaa uugaggagca guuuaaugcc aacuucggca ccuaucuuga ucucaaaaag     1800

uuugacgagc ugcaucccua ccuggaugac gaaaaauauc aagauacacu cgaggaagug     1860

aucaagguuc ugaccguguu cgaggaccgg agcaugauuc agaaucagcu ugagcagcug     1920

ccucucaacc uguccacaaa gaccaucaaa gcgcugucua gacgcaagua cacggggugg     1980

ggacggcuua gcgcuagacu cauugauggc auccacgaca aaaauuccgg caagacaauc     2040

cuggauuauc ugauugaaga cgagagcgac ucuuacaucg ucaaccggaa uuuuaugcaa     2100

cucauuaacg augaccaucu guccuucaaa aagaucauug aagauagcca gcccuacaag     2160

gagcagcaau cugccgaaga gaucgugucc gagcuuagcg gguccccggc caucaaaaag     2220

gggauucugc agucucucaa aaucguugac gaacuggugg cuauuauggg cuauaagccu     2280

aaaaauaucg ucguggagau ggcccgcgaa aaccagacca ccgggagagg aaagcagaau     2340

agcaagcccc ggcugaaagg cauugagaac gggcuuaagg aauuuuccga uucugugcuc     2400

aaaggcagcu ccaucgacaa uaagcaacug cagaacgaua gacuguaccu cuauuaccug     2460

cagaauggaa aagacaugua uacaggccac gagcuugaua uugaccaucu gagcaccuac     2520

gauaucgacc acaucauucc ucagucuuuc cucacagaua acuccaucga caaccgcguu     2580

cugacgacca gcaagucuaa ucgggggaaa uccgacaacg uccccagcga ggaaguggug     2640

agaaagaugg aucgcuucug gagaaagcug cuuaaugcua aacucauuuc ugagcggaag     2700

uauacaaacc ugaccaaaaa ggaacugaca gagagcgaca aagccggauu ucucaagcgc     2760

caacuggucg aaaccagaca gaucacgaag cacguggcga ccauucuuga uuccaaauuc     2820

aaugaggaca gcaacaaucg ggauguucag aucaucacac ugaagucugc ccucgugucc     2880

gaguuucgca aaaccuucaa ccuguacaag gucagagaaa uuaaugaccu gcaucacgcu     2940

caugaugccu accuuaacgc ugugguugcc cugagccucc ugcgggugua uccccagcuc     3000

aaaccugagu uugucuacgg cgaauauggg aagaauagca uccacgacca aaacaaggcg     3060

acaauuaaaa agcaguucua cucuaauauc acccgcuauu uugccagcaa agauuacauu     3120

aucaacgacg auggagagau ccuguggaau aagcaggaga cgauugcuca agugaucaaa     3180

acacuuggca ugcaccaggu uaacgugguc aagaaggugg aaauucagaa agggggcuuc     3240

uccaaggagu cuauccagcc gaaaggagaa agccaaaagc ugauuagacg gaaacagcag     3300

uggaacacca agaaguaugg cggguuugac ucccccgugg ucgccuacgc uauccuccug     3360

ucuuucgaca aaggaaagcg caaagcccgg agcuuuaaga uuguuggcau cacaauccag     3420

gauagagagu ccuucgaagg gaauccuauu cuguaccuua gcaaaaagga cuaucauaac     3480

cccaaagugg aggccauccu gccuaaguac ucucucuucg aguuugaaaa uggaagacgc     3540

cggauggugg cuuccgcgag cgagacccaa aagggcaacc agcugauuau ccccgggcac     3600

cucauggaac ugcuuuauca uucuaaaaag auuaucaaug gcaaaaacuc cgauagcguc     3660

ucuuacaucc agaauaacaa ggagaaauuc agagaaauuu uugaguauau cguggacuuc     3720

uccagcaagu acauuuccgc cgaugcuaau cugaacaaga ucgagaaaau uuuugaaaau     3780

aacuuccaca aggccagcga gcaggaaauc gccaaaucuu uuaucaaucu ccugaccuuc     3840

acagcuaugg gagccccggc ggacuuugag uucuuuggcg aaaagauucc ucgcaaacgg     3900

uauguuagca ucuccgagau uaucgaugcu guguucauuc aucaaucuau cacggggcug     3960

uacgagacca gaguccgccu uacagaagug uga                                  3993


<210>  4102
<211>  3993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4102
auggagaagg aauacaccau cggccuggac auugggacaa acagcguggg augggccguc       60

cucacggaug acuauagacu gguugcucgg aaaaugucca uccagggcga uucuaaucgc      120

aagaaaauua agaaaaacuu cuggggagcc agacuuuuug aggaagggaa gaccgcgcaa      180

uuccggcgca ucaagagaac aaaucggcgc agaauugcuc ggcgcagaca gcgggugcug      240

gcccuccagg acaucuuugc cgaggaaauc cacaaaaagg aucccaacuu cuuugcucgc      300

cuggaggagg gcgacagagu ggaagccgau aaacgguucg cuaaguuucc ugucuucgcc      360

acccuuagcg aggagaaaaa uuaccaucgc caguauccga caauuuacca ccugagacau      420

gaccucgcga acuccaagga gcaagccgau auccggcugg uguaucuggc uauugcccac      480

ugccucaagu acagagggca uuuucuguuc gagggagaac uugacaccga gaauacaucu      540

guuaccgaaa acuaucagca guuucugcag gcuuaccaac aguucuuucc cgagccuauc      600

ggcgaucucg acgaugccgu gcccauucug acggaacgcc uuagcaaagc gaagcggguc      660

gagaaagugc uggccuacua uccuuccgag aagucuaccg gcaauuucgc ucaguuccuc      720

aaacugaugg uugggaacca ggccaauuuu aagaaaacau ucgaccugga agaggaaaug      780

aagcucaacu uuaccagaga cuguuacgag gaagaucuga augagcuucu ggagaagaca      840

agcgacgauu augcugaacu cuuccugaaa gccaagggag uguacgacgc cauccuucug      900

ucccaaauuc ucagcaaauc ugaugacgag accaaggcua aacuguccgc gaacaugaag      960

cugcgcuuug aagaacacca gcgggaucuc aagcagcuga aagaacuugu cagacgcgac     1020

cugcccaaga aauaugauga cuucuuuaag aaucggagca aaaacggcua cgccggguau     1080

gugaagggaa aggcuacgca agaggauuuc uacaaauuuc ucagaacaga gcuggccggc     1140

cuugaagagu cucaguccau cauggaaaag aucgaccugg agauuuaccu ccugaaacag     1200

cgcaccuucg ccaacggggu gaucccucac cagauucauc uggucgagau gcgggaaauc     1260

auggacagac aaaagcgcuu uuauccguuc cucaaaggag cucagggcaa gauugagaag     1320

cugcuuacau uucggauccc cuacuauguu ggcccucugg cccaggaagg gcagagcccc     1380

uucgcgugga ucaaaagaaa gucuccuucc caaauuaccc ccuggaauuu cgcugaggug     1440

guggauaaag aaaacagcgc caucgaguuu auugagcgga ugaccaauca ggacacauac     1500

cucccgaagg aaaaaguccu gccuaagcag ucccugaucu aucagcgcuu caugauuuuu     1560

aacgagcuua cgaaaguguc uuacaccgau gaaagaggaa agagccacua uuucuccagc     1620

gagcaaaagc ggaaaaucuu uaaugaacuc uucaagcagc aucccagagu uacagagaaa     1680

cagcugcgca aguuucugga gcucaacgaa cagaucgacu cuaccgagau uaaaggcauc     1740

gaaacauccu ucaaugccag cuacucuacc uaccacgauc ugcuuaagcu gagcgaccaa     1800

auggauacgc uccuggacga uccugacaug acaaccaugu uugaggaaau uaucaagauu     1860

cugaccaucu ucgaggaucg ggagaugauu agagaacagc uuaaacccua ugagacagug     1920

cucgggcugc ccgcuaucaa gaaacuggcc aagaaacacu acaccggaug gggccgccuc     1980

uccgaaaaga ugauccaggg gaugagagag aagcaaucuc ggaaaacaau ucuggacuau     2040

cuuaucgaug acgacgauuu uccuugcaac cgcaauagaa acuucaugca gcugauuaau     2100

gacgaucauc ucagcuuuaa ggagacgauc gcuaacgaac ugauuauguc cgacagcaau     2160

guccugcuug aucaggugaa agccaucccg ggaucucccg cgguuaagaa aggcaucugg     2220

caguccauua agaucgugga ggaaauuauc ggcauuaucg ggaaggcccc uaaaaacauc     2280

gucauugaga uggcucggga aaaucaacgc accagcagau cucggccccg ccucaaggcc     2340

cuggaggagg cucugaaaaa caucgacucc ccucuccuga aggauuaccc cacagacaac     2400

caggcccuuc agaaagauag acuguaucuc uacuaucugc agaauggaaa ggacauguac     2460

accggcgaac cgcuggagau ucaccggcuu agcgaauacg auaucgacca uauuaucccu     2520

cgcuccuuca ucguggauaa cucucucgac aauaaagucc uggugagcuc caagguuaac     2580

agagggaagc uggacaaugc gcccgauccu cucgugguca aacggaugag aucucacugg     2640

gagaagcugc aucaagccaa acuuauuagc gacaagaaac uggcuaaccu cacaaagcag     2700

aaucugaccg aagccgauaa ggcucgcuuc auccagcggc agcuggugga gacgagacaa     2760

auuaccaaac acguugccaa ccuucugcac cagcauuuua aucuccccga ggaagugucc     2820

gccacagaga agaccagcau cauuacacug aaaucuaccc ucacguccca guuccgccaa     2880

auguuugaca ucuauaaggu ccgggaaauu aaccaccauc accacgcuca ugaugcguac     2940

cugaauggag ugguggccau gacacuucug aaaaaguauc cuaagcucgc ucccgaguuc     3000

guuuacggca gcuauaucaa aggggacauc aaccagauua auaaggccac cgccaaaaag     3060

gaauuuuacu cuaacaucau gaaauucuuu gaguccgagg aaauuaucug ugaugagcag     3120

ggcgaaguca uuuggaauaa gaagagagac cugagcacaa ucaaaaagac caucggagcu     3180

caccagguga acauugugaa aaaggucgag aaacaaaagg gcggguucua uaaggagacc     3240

aucaacucua aagccaaucc ggaaaagcug auuccucgca aagcgagccu ugauccccug     3300

aaguacggag gcuacgggag cccccucgug gcuuauacag uuaucuuuau uuucgagaaa     3360

ggaaagcaga aaaaggugac gaagggcauc gaagggauca ccgucaugga gcagcugcgg     3420

uuugaacagg acccuagaga guuccucaaa acaaagggcu acgagggagu gaaacaaugg     3480

cugauucuuc cgaaguauau ccuguucgaa gcccagggcg gguaccgccg gaugauugcc     3540

ucccaucagg agacccagaa agcuaacucu cucauccugc ccgaaaaucu gguuacacuu     3600

cuguaucacg ccagacauua cgaugagauu aaccacaagg uguccuuuga cuaugucaau     3660

gcgcacaagg aaggauucaa cgacaucuuu gauuucauua gcgacuuugg cgugcgguac     3720

auccucgcuc cucaacaucu ggagaaaauc aagguugccu acgagaaaaa uaaggaagug     3780

gaucucaaag agaugauuga cgccauccug ucccuucuga aguucacccu guuuggggcu     3840

ucugucgagu ucaaguuuuu cgauauuaaa auccucaagc gcuauaaaag ccugacggac     3900

auuugggaag ccacaaucau cuaccagucc gugaccggac uuuaugaaag acggguggag     3960

guccgcaagc ugugggaugg cgagagacuc uga                                  3993


<210>  4103
<211>  3996
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4103
augaacaagc agcccuacaa uaucggccug gacauuggga ccagcuccgu gggauggucu       60

gccacagagc ucgauaacag acugcuucac aucaaaggcc auaaugggau uggaguccgg      120

cuguucaagg aaggcgagag cgcugccgac cgcagagggu uucggacgac ccgcagacgg      180

cucgcgcgca gaaaauggcg gcugcgccuu cugaacgaaa ucuucgcuac agagauugcc      240

aagguugauc cuuccuuuuu cgccagacuc aaacaaucua augugagccc gaaggacccc      300

aacaagacca uguuugaaaa uauccuguuc gaugacgaga accuggauga ccagaaauuu      360

caccaugauu auaagacaau cuaccaccuc cggcaggcua uuaucaauca uccugagcag      420

aaauucgaca uucgccugau cuaucuugcc augcaccaca uuaucaagua cagaggacau      480

uuucugaacc aagcuaaugu gcaggauuuc aaaggcggca agauugaccu cgaaaagucc      540

uuuaaagccc ugaacgauau cuucgagaau caggggcggg accuucgccu ggucaccgac      600

aaggcgaacc aguaugugaa aguucucucu gauaaugcca agacaagaag cgaccggaaa      660

aaggaacugu ccaaacugcu cuacgugccc aucgauaagg acaccgauaa gaacagcaaa      720

aaggucacgg gagagauucu gaaagcuauc cuuggcaaca aggccaaauu ugacgugauu      780

uucgggaucg agguugaaaa uccuaaggag ugggugcuga ccuucaacuc ugaugacuuu      840

gaugacaaga uugaaggacu cuccagccaa augacagaug aggacucuga aauccugcuu      900

auccugaaag agcucuauuu cgcucugaau cuguccgaca uucucaacaa uagcgucacc      960

gauaagaacg ccaaaacacu gucugaggcg augaucaaua gauacgacga ucacaagaac     1020

cagcuugcca ugcugaaaaa ggugauugac uccagcgaua agaaacaugc uaaggaacuc     1080

aaagaggccu acuccgcuua uaucgacggc gaaaauaaca agaaaauuuc uuuugaggau     1140

cugaagaagc gcauccagaa aaaucuugug gacagcgaac ugucccagaa gaucaacgau     1200

cuccuggacc acgauuacuu ccugcccaaa caacggagca aggagaaugg ggccauuccu     1260

caccagcucc agcaacagga gcuggaccag aucauugaau cucagaaaca auauuacccg     1320

uuucuugccg agcugaaccc caaugaaaag agaucccgcc aggcuaaaua uaagcucgau     1380

gagcuggucg cguuccgggu uccuuacuau gugggaccca ugguggaagu caccgacagc     1440

aaccaggaga acaauggcaa guuugccugg augaucagaa aagagcaggg cgaaauuacg     1500

ccuuggaacu ucgacgagaa gguggaucgc acagaaaccg cuacaaaauu uaucacccgg     1560

augaccacaa cggacaccua ccugauuggg gagcccguuc uuccgaagaa uagccuccug     1620

uaccaaaaau ucgugguccu gaacgagcuc aauaaccuga agaucaauca ggaaaucauu     1680

acaccuggac agaagcagua ucuuuacgag caugugauga aaaagaacaa aagaguuacc     1740

cugaagaaaa ucgccgaugc ccucgugauu aagggcgacu aucccuacgc uccugaaguc     1800

aaggggacaa ccgaucagaa aaaucugaac aauggacuga cgacauauau cgaccuugcc     1860

aagauuuuug gcaacgagcu cgaugacgug gaucgcgaag cggacuucga gaaaaucauc     1920

gaguggagca ccauuuuuga agaucagcac aucuucgcuc ugaagcugca ggagcucaaa     1980

uggcugaccg aaaagcagcg gaaucaaguc gugaaacuua gauaccaggg guggggaaag     2040

cuguccaagg agcuccuggc cggccugguu gacgauaacg gccagcgcau uaucgaccuu     2100

cucuggucug acaaucaaaa cuucaugcag auugugaacc agaaaucuuu uaaggaaucc     2160

aucgccgauc auaauggggc ucaccuggag gagaaaaacc ugaaggacgu caucaaugau     2220

cucuauacaa gcccccagaa caaaaaggcc auucggcaag ugauccuggu uguggacgau     2280

auuaucaaug cugucggaua cgaacccgcc aacauuauga uggaguucgc gagagaagac     2340

ucuaaugauc aucggcuuac caacucccgc agcagacagc uggagaaugu guacaaggac     2400

aucacaaacu cuugguuuga uaaugacucc gugaaagaug aacucgagga caagaucaaa     2460

aacaaggaca aauucacgga ucggcuguau cuguacuuua cccagggcgg gaaggaccuu     2520

uauacaggag agccucucaa uauugaugac cugagcuccu acgauaucga ccacauucug     2580

ccgcagucuu ucauccucga uaacagccug gacaauagag uucuuaccuc ccaacgcaac     2640

aaccgggucu cuaaggaaga ucagcugccc ggcgagcucu uugggacaaa aaugcacgcc     2700

uucuggaaga gacugaaagc ugcccaucug auuagcaaua agaaauauua caaccuuacc     2760

cugacgccua auuccaucag caaguuuaac aaucagggcc gcuucauuaa cagacagcuc     2820

guggaaaccc ggcaagugau caagcuggcu gccgagaucc uccacaaucg cuauucugag     2880

gaaaaaggaa caaacauugu caccaucaag gcgaaucuga cacaucacau gagagagaaa     2940

uuuaacuucu acaagaaucg gaacgugaau gacuaccacc augccuuuga ugcuuaucuu     3000

accgccuucg uuggcaacug gcugcuccag caguacccca aacugaagcc uuauuucgug     3060

uacggggacu uugcuaagac ggaaauuaau aaccugaaau ccuucaauau gcuuuauaag     3120

uuugagcaag cccaccagcu gaacgaaaaa gauggaaaga ucgccaaaga cgcugacgcg     3180

cuccugggcu acaugaagaa cgucuaucau uucaagaaaa ugcucgugac aaagaaacug     3240

gagaccaauc acggggcccu uuuuaagcag acacuguacc ccagcccggc ucaugauauu     3300

aaaaagcgcc agcucaucaa accuaaggac aacagaccca ccgauaucua cggaggcuau     3360

acccacgagg aacggaagua caugucucug guuaaaguga uugggaagaa agacaaugag     3420

uaucgcaugg ccaacguccc ucugcuuaag aucaauaaaa acaaugauau uaaggaaauc     3480

cugaaguccc aauucagcaa caaaaaguuu gagauugugc uccccaaagu ucugaagaau     3540

caacucuucu cugaaaacgg cgcccuguuu gugaucggaa gcaaaggcua caaguauaau     3600

gcuaaccagc uuguccuguc cgaccuggau augaagcucc ugaaugaccu uagcaacaau     3660

aaaucuaucg augaggugac aauuaaccag cuguucaaga acaucgugga cuccacgaau     3720

aaauacaugc cucucuauga gauuaagaac cugaaugaag ucguugagaa auuuaacaau     3780

cucgaguuca acgauaagaa gaaaaccauc acagacuucc ugcuugccac ccacgcgaau     3840

cccacaauga gcaaccugaa gaauauuggg cugccguccu acuuuggaag auucaccucu     3900

ggcaacgcug ggaucaaacu cagcgaaaau gccaacguga uuuaccaauc cccuacggga     3960

cuguuuucuc ggaaggugaa aaucagcgau uucuga                               3996


<210>  4104
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4104
augaagaaca gagagaauau caaauacuau auuggcgugg acaucgggac caacagcguc       60

ggaugggccg uuauugauga aaauggcaac cugcucaaga aagggaagca ccaucugugg      120

ggaucccggc uuuucgacca ggcucaaaca gcccagaauc gcagaaacua ccggucuagc      180

cgcagacggu auaauaaacg cagacagcgg aucggccugc uccgccugau uauguccgau      240

auggugcuug agguggaccc cagcuuuuuc aucagacugg aaaagacgac cuuucucgau      300

aaggaggaca aaaaggcgau ccugaaagau aacuacaaga ugaauuauaa ccuguucugc      360

gacgaagauu acaaugacaa aaaguauuuu aaggauuacc cuacaauuua ccaccuccgg      420

aaaaagcugu gugagagcga cgagaaagcu gauccgcgcc uuaucuaucu ggcccuccau      480

cacauuguca aguacagagg gaacuuccug uaugaaggac aggagcuuca ucuggaaccc      540

uccaauaaag aggaggaccu caagauccug uuugacauuc ugggcaaaaa caaugauacc      600

guguacgaca ucucugaaga acaaauucag uucauccuca agacaguugu ggaaaacauc      660

agcaagaccg ccaaagucga ugagugcaug ucccagcuga agcugaacag cgaagacaaa      720

aagauuguga aagaguuuau gcggggccug guugggaaua aguucaacgu gucuaagcuc      780

uauaugcacg aggaucugca guuugacgau gaagaccuua aacugcaauu cuccgauaag      840

agcuacgagg aaaagaucac agaguaugaa aauguccucg aggagaagau ggaauuuauu      900

gaccugaugc agcgcuucua cucuuggauc gagcugucca aaauuguggg aagcgauucu      960

cagcacgcuu ccaucagcgg cgccauggug aacaucuacg aaucccauag agaggaccuc     1020

cggacccuga aggaagucau gcuuaagauu gggaaaaagg aguaugacga gauguucaaa     1080

ccuacgucua agaauguugu gaacuacuau aauuacguga accccgucgc uuguagcgga     1140

gauaaaaccg acggcuuuua uaaguacgug aagaaagcca ucgaaaaguc cgaugacagc     1200

agaaaagaug agauucugca gaagaucgcg aaugaaacau auaugcucaa acaaaccucu     1260

aagaacaaug ccuacauucc uuaccagaug cagaaagacg agcugaucaa gauccuugau     1320

caccaagaga aguauuaccc cguucugaaa gaaaaccgcg acaagauuau cuccauucuc     1380

gaguuccgga ucccuuauua cuaugggccg cuggauggaa auaaacaguu uggcuggcug     1440

auuaagaaaa agggcaagga aaacgagaga auccuccccu ggaaucauca ggaaauugug     1500

gacguccagg agacagcugc ccaauucauc aaaaagcuga ccaacuacug cacguaucuu     1560

ccuaucgaga aagugaugcc ccagaaaagc cugacauguu cuauguacga aguucucagc     1620

gaggugaaca aaauucgcau cgaugggaag cugcugccua uugacaccaa gaaucggcuu     1680

aucgaagauc ucuuuuucaa aagaaagaca gucaaagaga aggaccugau uaacuggcug     1740

aaacagaauc agcucaccgu gggagaaauc accggcuacc aaaaggagaa ggcuuuuucc     1800

ucuagccugg cccccuggau cgacuucaaa gagauuuuug augaaaucaa cgacuccaau     1860

uaugaucuua uugagaagau cauugaagac augacaaucu ucaacgaggg gagcauccug     1920

aaagaacgcc ucaagaaagc guacaaucug gaucagaaca aggucaaaaa gauuaugaag     1980

cugaaauauu cuggaugguc ccggcuuagc aagaaacuca ucaauggcau uagagccgac     2040

aacaaguuug ggucuuccgu gagcauccug gauguuauga aagaguccca cauggcucug     2100

auggagauua ucaaugacca ggaucucgga uucaagcaga ucauugaaaa ggagaacuuu     2160

aaaaaugaaa cgggcucuuu cgaguacgag gacaucgaaa accuggccgg cagcccggcu     2220

cuuaagcgcg ggauuuggca aacccuucag gugaucgagg aaauuaaaaa uuauauggga     2280

cacgagccua agaacaucua cauugaguuc gcccgggagg agcaggaaaa agucagaaca     2340

accacacgcg ugaagaagcu caaauccauc uauaacgaua ucaagaauca gcuggacguu     2400

cauggcaaag agguguacuc uaaccugaau aagcaagaug aaaaaucuuc cauugagaag     2460

cggcuuuacc ucuauuacac ccagcugggg aagugcaugu auagcggaga aucucuggac     2520

aucgacaaac ucuccaacua cgagauugau cacaucuuuc ccagaacgcu gacaagcgac     2580

gauucucuug acaaucuggu ccucgugaag aaaaaggaga accagcggaa acuggaugac     2640

cuggugcuuc cucucgaaau ucgcaauaag auggagguuu ucuggaagaa acuguaugau     2700

aacggccuga ucacccaaac caaguacuau agacucaugc gggacgaauu uagacgcgau     2760

cagaucgaca aauucauuaa ucggcagcug gucgagacaa gacagaucau uaagcaugug     2820

gccaacauca uugaaaauca cuacgaggau accaaagugu uuacaguccg cgcuaaccuu     2880

ucccaugagu ucagagaaaa guacgggauc uauaaaaauc ggaacgugaa ugacuuucac     2940

cacgcgcaug acgccuacau cgcuuguauu aucggccgcu auauucaaau cagauucccc     3000

ggacuggagg ccaaguacgu uuauggccag uacaugcaga acgugaagaa aacgaagaac     3060

aauauugaua aagaaaacca cggguuuauc auuaauagca ugaaguauga gugcaucgac     3120

gaggauaccg gagaagucau cuggaacccc gagcggauuc ucgacuacau caaauguuuc     3180

aauuaccgcg auguguauau uacaaagaag cuggacacca acaauagaga acuguuuaac     3240

guuacaaucc uuccuaauga uaaaaacucu gagaagggca aaaccaaggc cacgauuccg     3300

gugaauaaac ugcggagcaa cguccgcaag uacgggggau ucuccggccu ccagagcgac     3360

aucguggcua ucgaagggaa gaaaggcaag aaaauugaua gacggcugau caaucucccc     3420

auucugcuuc gcuauguuuc uaucgaggac caaugcaagu acauuaugga gaacaauaaa     3480

uauuccgaug ugaagaucau caagaaaauu aagaaaaacc agcugaucga aauugacgga     3540

aucuguuacu aucucaagag cgccgaugag cuggacaccg cguaccagcu gauucuuucc     3600

agagacgaua augaaaugau cuaccggauu gacagagcua uccagaacaa cgauuuuucu     3660

uauuucgaca auaaaaagga gaacaucgau gaacuguacc gccucauuug ccauaaaaug     3720

aucgagaauu auccuaagua caagaaaaag gucuaugagu ucauuaaaga acgggaggaa     3780

gaguuuuucg aacuggacaa cagccaaaag ugcaucguga uuuuugagau gaucagagug     3840

augaaacgcg gcgagcagua caucaauauu cccuucgaac cuuauaaccu caagaaugau     3900

cggaagggga gacugaacug ucaguccauc cuucuggaca auacauacuu uuacgauguc     3960

ucuauuaccg gaaucuauag caaaaaguac aaacucuga                            3999


<210>  4105
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4105
augcagaaga acuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

augaaagaug acuauacgcu caucagaaag cggaugaaag uucugggcaa caccgauauu      120

aagaaaauca agaagaauuu cuggggagug cgccuuuuug acgagggaga aacagcuaaa      180

gagaccagac ugaagcgggg cacacgcaga cgguaccaac gcagacggaa ccgccucauu      240

uaucugcagg auaucuucca gcagcccaug cuugccaucg acgaaaauuu cuuccacaga      300

cuggaugacu ccuuuuucgu gccugaugac aaaucuuacg aucggcaucc gauuuuuggg      360

agccucgagg aagaggucgc guaucacaac accuacccca caaucuauca ucugcgcaag      420

caccuggcug acaauccuga gaaagccgau cucagacugg uguacaccgc ccuugcucau      480

auuguuaagu accggggaca cuuccugauc gaaggcgagc ucaacacgga aaauaccucc      540

auuucugaga cauuugagca auuccuggac accuauagcg acaucuuuaa ggaacagcuu      600

gugggcgaua uuuccaaagu cgaggaaauc cugucuagca agcagucccg cagcagaaaa      660

cacgagcaga ucauggcccu cuuccccaac gaaaauaagc uggggaacuu uggacgguuc      720

augaugcuga uugugggcaa uacaucuaac uucaaaccug uuuuugaccu cgaugacgag      780

uacaagcuga aacuuuccga ugagaccuau gaagaggacc uggauacgcu ccuggggaug      840

acagacgaug uguuccuuga cgucuuuaug gcugccaaga auguguacga ugcgguggaa      900

augagcgcca ucauuucuac cgacacagga aacuccaagg cuguccugag caaccaaaug      960

aucaauuucu augacgagca uaaaguugau cucgcccagc ugaagcaguu uuucaaaacc     1020

caccuucccg acaaguacua ugaaugcuuu ucugauccuu ccaaaaacgg cuacgcuggg     1080

uacauugacg gaaagaccaa ucaggaggau uucuauaagu acaucgagaa agugaugaag     1140

acaaucaaaa gcgacaagaa agauuauuuu cucgacaaga uugauagaga aguguuccug     1200

cgcaagcaac gguccuuuua caacucuguc aucccgcauc agauucaccu ucaggagaug     1260

caagccaucc uggacagaca gagccaguau uaccccuucc ucgcggaaaa ucgcgauaaa     1320

auugaguccc uggugacguu ucggaucccu uauuacguug gcccccuuac cgugagcgac     1380

cagucugaau uugccuggau ggagagacaa uccgaugagc cuauccgccc cuggaacuuc     1440

gacgaaauug ucaauaagga gcggagcgcu gaaaaauuua ucgagagaau gacaaacaug     1500

gacaccuacc ugcucgagga aaaggugcug ccgaaacgcu cucugcucua ucagacauuc     1560

gagguuuaca augaacugac caaggugcgg uauacgaacg agcagggcaa gacagaaaaa     1620

cuuaauagac agcaaaaggc cgagauuauc gagacccugu uuaaacagaa gaaccgcguc     1680

cgggaaaaag auauugcuaa uuaccucgag caguaugggu acguggacgg aaccgauauc     1740

aagggcgucg aagacaaauu caacgccagc cuguccacau auaaugaucu ggccaagauu     1800

gacggggcua aggcguaccu ugaugacccu gaguacgccg auguguggga agacaucauc     1860

aaaauucuca ccaucuuuga ggauaaggcu augcggaaaa agcagcugca aacauauucu     1920

gacacgcuga gccccgagau ucucaaaaag cuggaaagaa agcacuacac cggauggggc     1980

cgcuucucca aaaagcugau caacgggcug agagaugagg gaagcaacaa aacaauucuc     2040

gacuaucuga agucugacga aggcuccagc ggcccuacca aucggaacuu uaugcagcug     2100

aucagagaua auacacuuuc uuucaaaaag aagaucgagg acgcccagac cauugaagau     2160

acgacccaua ucuacgacac aguugccgag cuccccgggu cccccgcuau uaaaaaggga     2220

auccgccagg cccugaaaau uguggaggaa aucaucgaua uuaucggcua ugagccugaa     2280

aacauugucg uggagauggc gcgggagagc caaaccacaa agaaagggaa ggaccugucc     2340

aaggaaagac ucgagaaacu gaccgaagcu aucaaggagu uugauggacc gucugacguu     2400

aaagugaagg aucuuaaaaa ugaaaaccug cgcaaugaca gacucuaccu guauuaccug     2460

cagaacggcc gggauaugua cacgaaugag ccccuugaca uuaacaaucu cagcaaguau     2520

gacaucgauc acaucauucc ucaguccuuc acaaccgaca acucuaucga uaauaaaguc     2580

cuggugagcc gcacaaagaa ccaagggaau aaguccgacg augugcccag cauuaacauc     2640

guucauaaaa ugaagccuuu uuggagacag cugcacaaag ccggccucau uucugaccgg     2700

aaguucaaaa accugaccaa ggccgagcau ggaggccuua ccgaagcuga ucgcgcccac     2760

uuucugaaua gacagcucgu cgagacacgg cagaucacga agcacguggc uaaccugcug     2820

gacucccaau acaauaccgc cgaagaacag cgcauuaaca ucgugcuucu caaaagcucu     2880

augacaucca gauuccggaa ggaguucaaa cuguauaagg uccgcgagau caaugauuac     2940

caucacgggc augacgcgua ucugaacgcc gugguugcua ccacaauuau gaaaguguac     3000

cccaaucuca agccgcaguu ugucuaugga caguacaaga aaaccagcau guucaaggag     3060

gaaaaagcca cggcuagaaa gcacuuuuac ucuaacauca caaaauucuu uaagaaggag     3120

aaagugguua augaagagac cggcgaaauu cugugggaua ccgagcggca ccuuagcaca     3180

aucaagagag ugcuguccug gaaacaaaug aacauuguca agaaagugga gaagcagaag     3240

gggcagcucu ggaaagaaac caucuauccu aagggagaca gcucuaaacu gauccccguu     3300

aaggagggca uggauccuca gaaauacggg ggccuguccc aagugagcga agccuucgcg     3360

gucgugauua cacaugagaa ggggaaaaag aagcagcuua aauccgaccu gaucucuauu     3420

cccaucgugg accagaaggc cuaugagcaa cacccuacgg cuuaccucga agaggccggc     3480

uauaauaacc ccaccguccu gcaugaacuc uuuaaauacc agcuguucga gcuugaagau     3540

gggagccgcc ggaugauugc uuccgccaag gaguuucaga aaggaaauca gaugguucug     3600

ccgcucgagc ugguggaacu gcuuuaucac gccaacagau acgacaaggu gaaguucccu     3660

gauucuaucg aguacgucca ugacaaucug gcuaaauuug augaccuccu ggaauaugug     3720

aucgauuuca gcaacaagua cauuaacgcg gacaaaaaug uucaaaagau ccagaaaauu     3780

uauaaggagc acggcacaga agauguggag cucaccgucg aguccuucgu gaaccugaug     3840

acauuuaccg ccaugggggc ucccgccacg uucaaguuuu acggagaauc uaucacccgc     3900

agccgguaua cauccauuac cgaguucaga ggcagcacac uuaucuuuca gucuauuacc     3960

gggcuguacg aaacacgcua uaaacucgag gacaauuga                            3999


<210>  4106
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4106
augaugaagg aguacagcau cggccuggac auuggguccu cuagcguggg auucgucgcc       60

guugaugacc aguauagagu ggugcgggcu aaaggcaaga acgucaucgg gacccgccuc      120

uuugaugaag gaaaaacagc cgcggacaga cggggccugc gcacgaccag acggcgccuu      180

uccagacgga aguggcgccu gagacuccug cgggggauuu ucgagcccca caucaaugaa      240

auugaugagu cuuucuucau gcgccuuaaa gaaagcaaca ucuccccuaa ggacucuaau      300

aagcauuuuc cgggagauau ccuguucaac gacagaagcg aucgggaguu uuacgagaau      360

uaucccacaa uuuaccaccu ccgccaagcu cugaaaaccg aaaagagaca guucgacauc      420

cgggagauuu aucuggccau gcaucacauc gugaaauaca gaggccauuu ucucgaaggc      480

gggacaguua ccgauuucga ggugggagag cuggaccuug ccggcaaguu uucccgccug      540

aacgaacucu ucgagagcau uaaucuggaa gucccuuuug ugcuucggcu ggauaaccuc      600

gaggacguua aagcugugcu gaucgacucu cagagauccg cccaggaucg ccaacggaca      660

cugacccccg acauuuaugu cccuagcacg gauaagauca ccgaaaagag auucaaaggg      720

aucgcuacag agcuccugaa ggccauugug ggaaauaaag cgaaguucga cgugcuugcc      780

ggccuggagc ucgauaaaga agaggcuaag cugugggccc uuaccuuuga cucugaaaca      840

uucgaugagg acaucgaaca gcuggagggg gauaugaccg aggcuacgca ggaccucauu      900

gaaauccuga aaucccugcg cagccucauu cggcuggccg ucaacaugcc cgagggaguu      960

ucucuuuccg aaaugaugau ccagagauac gaggaucacg cggaccaccu gaagcuccug     1020

cgcgacauca uucauaauac agaugccgaa aaggcugccg gccuucaagc ugccuacgac     1080

ggcuauaucg auggggugaa aggaaagaaa accccugugg acgauuuuua caaggccauu     1140

aaaaagaacc uggacgauag cgaggcugcg caccagaucc ucgcccugau ugacaagaau     1200

gaguucaugc cgaaacagcg guccaagucu aacggcugga ucccccauca gauccaacag     1260

aaagaacugg auagaauuau cgagaaucag aagcaauauu acccuuggcu cgcugacguc     1320

aaccccaacg aaagccgccg gaaaguggcc ccuuauaaga uugaugagcu gguuacauuu     1380

agagugcccu acuaugucgg gccgcuugug accuccgagg aacagaagaa auacggaagc     1440

aaguucgccu ggaugguucg gaaagaggaa aagggcgaga ucaccccuug gaauuuugac     1500

cagaaagugg aucgcgcugc cucugcgaca gaauuuauua acagaaugac gaccauggac     1560

acauaccugc ucaaugagga cguccugccc gcucacuccc ugaucuauca gaaguacgag     1620

gugcuuaacg aacucaauaa ggucaaaauu aacggggaug ccaucacccc ugagcugaag     1680

caaaagaucu aucacgaccu guuugaaaag cagacaaccg ugagcauuaa aaaucuccag     1740

acguaccugg uuucugagag cgaagccucc gaggugcccg agaucaaggg acuugcugau     1800

gagaaaaagu uccugucugg ccucacaacc uaucaugacc ugaugaagau uuuugaucgg     1860

aacgucguga augaccccga gagagccgaa gauuucgaga aaaucauucg cuggagcacc     1920

guuuuugaag acaagcacau cuaccgggag aaacuggauu cccuuagcug gcucacaacc     1980

gaccagaagg auagacuggc ugugaaacgc uauagaggcu gggggcggcu gucugagaag     2040

cuccugaucg gacuucgcga cgaaggccau agauccauua uggauuaccu gugggagaca     2100

acgagcaacu ucaugcaaau ccaggcccag aaugacuucu cugaaaagau ugcggccgag     2160

aacaaagagu uucucgacaa uacccagucc guccaaggga ucauuaacga acuguacaca     2220

agcccucaga auaagaaagc uauccggcag acccugcuug ugguggagga uaucaagaaa     2280

gccuuccacg gagaagcucc gaagcgcauu uuuguugagu ucgccagaga aaacgagucc     2340

acaccccagc ggucuaucca acgccagaga caggucgaga acgcguauga aaaggugacc     2400

caaaaucucc ugguggacga uaaaauucgg gccgagcuga aggacagcau caacgaaaaa     2460

cgccucuuuc augauagacu guuccuuuac uuuaugcagg gcgggauuga cauguauucc     2520

ggcaagaaaa ucaauaucga uaaccugucu gcuuacgaca uugaucacau ccucccucag     2580

agcuucacgg ucgacaauuc ccuggauaac cgggugcuga ccaaucagaa ggacaacaga     2640

gagaagggaa auagccuucc cucugaacuc uaucgcggcu ccaugaaaga gacaugggag     2700

augaugcgga accaagggcu gcugagcaag ggaaaauuuc acagacucau gaugaccccu     2760

aauucuauga cagccuacga ccagcagggc uucauuaacc gccagcuggu ugaaacccgg     2820

caagugauca aacuugcugu cgagauucug gccuccgaau uugaucaggc cgacgagaaa     2880

guggaaaucg uuagcgugaa ggcugggcuc acgcaucaga ugagacagga uuucucucug     2940

auuaaaaauc gcgacgucaa cgauuaucac caugcguuug acgccuaccu gacagcuuuc     3000

gugggaaccu accuucugaa gcgguauccc aagcucagac cguacuucgu uuauggcgag     3060

uuuaaaaagu ucagccaaaa acaggccaau cugacaaacu uuaacuuccu cugggcccug     3120

aagcacaaag aucagguggc ugucgaggac accggggaaa agaucuggaa uaagccucaa     3180

gaucuugccu acauccgcca ccuguuugcg uucaaaaaga uucucaucuc ccgggaggug     3240

agcgaaagac auggcgcucu guuugaccag acccuguaua aagccucuga ugacaaggcc     3300

uccggacagg gcagcaaaaa gcugauuccc gcuaaggaua accggccuac agagcuuuac     3360

gggggauauu ccggccagac gucugccuuc auggcgaucg ugaaagucac caaggggaaa     3420

aagacaaaau accucguugu gggagugccc accgcugcca uuagccaacu gcagggcaug     3480

gacgucaagu ccucucagua cacagagaag aucuauaaua uccucagccc uaaacugcuu     3540

uccaagaacg acgauuuuga agugauuguu cccaaagugc gcuacagaca gcugauccuc     3600

gacaauaacc aacuguucac ccuggggucu gauacguaua aguacaaugc ccggcagcuu     3660

guccugagca acgaggugcu cgcuguuauu aaucgcgaca aauccuuuau cgauaagaag     3720

cugagcgaag aggccgaaaa ccaggcucuc aaugacgccu ucgaugagau uguggucgcg     3780

auggacaaac acuucgaucu guaugacaag aacggcuuua gagagaaagu gaaggccgga     3840

aaacagcggu ucgaagaacu uccgguuucu cuguuuuccg aagauaagga gaaugaaggc     3900

aucacaaaaa gaaccgugcu gaagcgcauc cuccaagggc ugcaugcuaa cgccaccaca     3960

agcgaccuua agguccugga uggaaaauuc uacaauuga                            3999


<210>  4107
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4107
augaagaacu acaccaucgg ccuggacauu gggguggcca gcgucggaug gguuugcauc       60

gaugagaauu auaaaauucu caacuacaau aacagacacg cuuucggcgu gcaugaauuu      120

gaguccgccg aaucugcggc ugggcggcgc cugaagagag gaaugcggcg cagauauaau      180

cggcgcaaaa agagacuuca gcugcuccaa agccuguucg acuccuacau cacagauucu      240

ggcuuuuuca gcaaaacgga cucccagcac uuuuggaaga acaauaacga guucgaaaau      300

cggucucuua ccgaggugcu gagcucccuc cgcauuagcu cuagaaagua ucccacaauc      360

uaccaucugc gguccgaucu gaucgagagc aacaaaaaga uggaccuccg ccuggucuau      420

cuugcccugc acaaucucgu gaaauacaga gggcauuuuc ugcaggaagg aaacuggucu      480

gaggccgcuu ccgccgaagg cauggaugac cagcuucugg agcucguuac ccgguacgcu      540

gagcuggaaa aucugagccc ucucgaucug ucugaguccc aauggaaggc cgcggaaaca      600

cuucugcuca accgcaaucu gaccaaaaca gaccagagca aggagcuuac cgccauguuc      660

ggcaaggaau augagccguu uuguaaacug guggcugggc ucggagucuc ccugcaccag      720

cuguuccccu cuagcgagca ggcccucgcu uacaaggaaa cgaaaaccaa ggugcaacug      780

uccaacgaga acguugaaga ggugauggaa cuucugcucg aggaggaaag cgcccugcuu      840

gaggcggucc agccuuuuua ucaacaggug gugcuguacg aacuccugaa aggcgagaca      900

uaugucgcca aggcuaaagu uucugccuuc aagcaauacc agaaggauau ggcuucccug      960

aaaaaucucc uggacaagac cuuuggggaa aaaguguaua gaagcuacuu cauuucugau     1020

aagaacagcc agcgggagua ccaaaaaucc cacaaggugg agguccuuug caagcuggac     1080

caguucaaua aagaagccaa guuugccgag acauucuaua aagaccucaa gaaacugcug     1140

gaagauaagu cuaagaccag caucggaacg acagagaaag acgagaugcu uagaauuauc     1200

aaggcuauug auuccaacca guuucuccag aaacaaaagg gcauccagaa ugcggccauu     1260

ccccaucaga acagccugua cgaagcugag aaaauccugc gcaaucagca agcccacuau     1320

ccuuucauca ccacagaaug gauugagaag gugaagcaga uccucgccuu ucggauuccc     1380

uacuauaucg ggccucuggu uaaagacacc acccagucuc cguucuccug gguggaaaga     1440

aagggagaug cucccauuac accuuggaac uuugacgagc agaucgauaa agccgcgagc     1500

gcugaggccu ucaucucucg caugcggaag acguguaccu accuuaaagg ccaagaaguc     1560

cugcccaagu ccagccucac auaugagaga uuugaagugc ugaaugagcu gaacggcauu     1620

cagcuucgca ccacaggggc cgaauccgac uuccggcaua gacucucuua cgagaugaaa     1680

ugcuggauca uugauaaugu uuuuaagcag uacaagaccg ugagcacgaa acgccugcug     1740

caggagcuca agaaaucccc uuaugcugac gaacuguacg augagcacac aggagaaauc     1800

aaggaggucu ucggcaccca aaaagaaaac gccuuugcua ccucucuuag cggguauauu     1860

uccaugaaaa gcauccuggg agccgugguc gacgacaauc ccgcgaugac agaggagcuc     1920

aucuacugga uugccguguu cgaagaucgg gagauccugc accugaagau ucaggaaaaa     1980

uauccgucua ucaccgacgu ucagagacaa aaacuugcuc ucgugaaacu gccuggcugg     2040

gggcgcuucu cccggcugcu cauugaugga cugccccuug acgagcaggg ccagagcguc     2100

cuggaucaua uggagcagua cucuuccgug uuuauggaag uucucaagaa caaaggcuuc     2160

gggcuggaga agaagaucca gaaaaugaau cagcaccagg uggacggaac aaagaaaauu     2220

agauaugaag auaucgagga acuggccggc agcccugcuc uuaagcgggg gaucuggcgc     2280

ucugucaaaa uuguggagga gcucgugagc aucuuuggag aacccgccaa cauuguucug     2340

gaggucgcga gagaagacgg cgagaagaag cggacgaaau ccagaaagga ucagugggag     2400

gaacugacca aaacaacccu caagaacgac cccgaucuga aaucuuucau cggggagauu     2460

aagagccaag gcgaccagcg cuuuaaugaa cagcgguucu ggcuuuacgu gacacagcaa     2520

ggaaaauguc uguacaccgg caaggcccuc gauauccaga accuguccau guaugaggug     2580

gaccauaucc ugccucagaa uuuugucaag gacgauagcc uugacaaccu ggcucucgug     2640

augccggaag ccaaucaaag aaagaaccag guugggcaga auaagaugcc ccuggagauu     2700

aucgaagcua accagcaaua cgccaugcgc acgcucuggg agagacugca cgagcuuaaa     2760

cugauuuccu cuggaaagcu cggccggcug aaaaagccua gcuucgauga aguggacaag     2820

gauaaauuua ucgcccgcca gcuggucgag accagacaga uuaucaagca ugugcgggac     2880

cuucuggaug aacgcuucuc caaaucugac auccaccucg uuaaggcugg gauugugagc     2940

aaauuuagac gguucuccga gauccccaag auucgcgauu auaauaacaa gcaccaugcg     3000

auggacgccc uguuugcugc cgcccucauc cagucuauuc ugggaaaaua cggcaagaau     3060

uuccuugcuu ucgaucugag caaaaaggac agacaaaaac aguggcgguc cgucaagggg     3120

agcaacaagg aguuuuuccu cuuuaaaaau uucggcaacc ugcgccugca gucuccugug     3180

acaggagaag agguuuccgg cguggaauau augaagcacg ucuacuuuga gcuucccugg     3240

cagaccacaa aaaugaccca aacgggggau ggaauguucu auaaggaaag caucuuuucu     3300

ccgaaaguga agcaggccaa guacgugucc ccuaaaacag agaaguucgu ccaugacgag     3360

guuaaaaauc acagcaucug ccugguggaa uuuaccuuca ugaagaaaga gaaggaagug     3420

caggagacaa aauuuauuga ccucaagguc aucgaacacc aucaguuccu gaaggagccc     3480

gagucucaac ucgcgaaauu ccuggcugaa aaggagacca acuccccuau uauccacgcc     3540

agaauuaucc ggaccauucc caaauaccag aagaucugga ucgaacauuu uccuuauuac     3600

uucauuagca caagagagcu ucacaacgcc cgccaguuug aaaucuccua ugagcugaug     3660

gagaaaguga agcaacugag cgaacggucu agcguugagg aacucaagau uguguucggc     3720

cugcuuaucg aucagaugaa ugacaacuac cccauuuaua cgaaauccuc uauccaggau     3780

agaguccaga aguuugugga cacccaacug uacgauuuca agagcuuuga gaucggguuc     3840

gaggaacuca agaaagcugu ugccgcgaau gcucagcgcu ccgacacauu uggaucucgg     3900

auuagcaaga agccgaaacc ugaggaagug gccaucggcu augaguccau uaccgggcug     3960

aaguacagaa aaccccgcuc ugucgugggc acaaagcggu ga                        4002


<210>  4108
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4108
augagccaga acaucgugga cuacugcauu ggccuggauc ucgggaccgg auccgucggc       60

ugggccguug uggacaugaa ucacagacug augaagcgga acgggaaaca ucuuugggga      120

ucucgccugu ucagcaaugc ugagacagcc gcgaacagac gggcuucccg cucuaucaga      180

cggcgcuaua auaagagacg ggaacgcauu agacuccugc gggccauccu ucaagauaug      240

gugcuggaga acgaccccac guuuuucauu cgccucgaac acaccagcuu ucuggaugag      300

gaagacaaag ccaauuaccu gggcgcugau uauaaggaca acuacaaucu cuucaucgau      360

gaggacuuua acgauuauac auacuaucau aaauacccua ccaucuacca ccugagaaag      420

gcccuuugug aguccacaga aaaggcugac ccgcggcuga uuuaucucgc ccugcaucac      480

aucgucaaau accgcgggaa uuuccuuuau gagggacaga aguuuaacau ggaugcgucu      540

aauauugaag acagacugag cgacguguuc acccaguuug ccgauuucaa caauaucccc      600

uacgaggacg augagaaaaa gaaccucgaa auucuggaga uccugaaaaa gccucucucc      660

aagaaagcua agguugacga agugauggcc cugauugcuc ccgagaaaga uuuuaagagc      720

gccuauaaag aacuugucac aggcaucgcg ggcaacaaga ugaaugugac caaaaugauc      780

cugugcgagc cuauuaagca gggggacucu gagaucaagc ucaaauucuc cgauagcaac      840

uacgacgauc aauuuucuga aguugagaau gaccugggag aauaugugga guucauugau      900

ucccuucaca acaucuacag cugggucgaa cugcagacga uuaugggcgc cacccauaca      960

uacaaugcuu cuaucuccga ggccauggug agccgguaua acaagcacca ugaggaccuc     1020

cagcugcuga aaaaguguau caaagacaau gugcccaaga aguacuucga uauguuuaga     1080

aacgacuccg aaaaacucaa gggguauuac aauuauauua accacccuuc uaaagcuccg     1140

gucgaugagu ucuacaagua uguuaaaaag ugcaucgaaa agguggacac ccccgaggcc     1200

aaacagauuc ugcacgauau cgaacuugag aauuuucugc ucaagcaaaa cagccgcaca     1260

aauggauccg ugccuuacca gaugcagcug gacgagauga uuaaaaucau cgauaaccag     1320

gccaaguacu aucccguccu uaaagaaaag cgggagcaac ugcucagcau ucugaccuuc     1380

aagaucccuu acuauuuugg cccccugaau gaaacgucug agcaugcuug gauuaaaaga     1440

cucgagggga aggaaaacca gcgcauccug ccguggaacu accaggacau uguggauguu     1500

gacgcgacag ccgagggauu caucaaacgg augagauccu auuguaccua cuuuccugau     1560

gaagaggugc uucccaagaa uagccugauu gucucuaaau augaagugua caacgagcuc     1620

aauaagaucc gcguugacga uaaacugcug gagguggaca ucaagaacga cauuuacaau     1680

gaacuuuuca ugaacaauaa gacagucacc gagaaaaagc ucaaaaacug gcuggugaau     1740

aaccaaugcu guaauaagaa cgcugaaauc aaaggcuuuc agaaggagaa ucaguucagc     1800

accucccuga caccuuggau ugauuuuacg aacaucuucg gcgaaauuaa ucagucuaac     1860

uuugaccuca ucgaggauau cauuuaugac cugaccgucu ucgaggauaa gaaaaucaug     1920

aagcggagac uuaaaaagaa auacgcccug cccgacgaua agauuaagca aauccucaaa     1980

cugaaguaua aagacuggag ccgccugucc aagaaacuuc ucgaugggau uguggccgac     2040

aacaaguucg gaagcucugu uacagugcug gauguccugg aaaugucccg gcucaaucug     2100

auggagauca ucaacgacag agaucuuggc uacgcucaga ugauugaagc cgcggcuagc     2160

ugccccgagg acgggaaguu uaccuauaaa gaagugcagc gccuggccgg aucuccugcc     2220

cucaagcggg gcaucuggca gucccugcaa auuguugagg agaucacaaa agugaugaag     2280

uguagaccga aauacauuua uaucgaguuc gagcggagcg aagagaccaa ggagcgcacg     2340

gaauccaaaa ucaagaagcu ggagaauguc uacaaagacc uugaugaaca gacaaaggug     2400

gaguacaaaa ccgugcucga agaacugaag ggguuugaca acaccaaaaa gauuucuagc     2460

gauucccugu uccucuauuu uacacagcug ggaaagugca uguacucugg caaaaagcug     2520

gacaucgaua gccuugacaa auaucagauu gaucacaucg uuccccaauc ccucgucaag     2580

gacgauagcu ucgacaauag agugcuggug gucccuucug agaaccagcg gaaacuggau     2640

gaccuugugg uucccuccga cauuagagug aagaugaaua gcuuuuggaa gcuccuguuc     2700

gaucaugaac ugaucucucc uaaaaaguuu uacucccuca uuaaaaccga guauacagaa     2760

cgcgacgagg aacgguucau caacagacag cuggucgaga cgcgccagau caccaagaau     2820

gugacacaaa uuaucgagga ucacuacagc accacaaaag uugcugccau uagagcuaac     2880

cuuucucaug aauuucgggu gaagaaucac aucuauaaga accgcgacau uaaugauuac     2940

caccaugccc acgacgcgua caucgucgcc cugaucggcg gguucaugag agaucgguau     3000

cccaacaugc augacagcaa agcuguguac uccgaguaua ugaagauguu ucgcaaaaau     3060

aagaacgaua aaaagagaug gaaggacgga uucguuauua acagcaugaa uuacccguau     3120

gaaguggaug gcgagcucau cuggaacccu gacauuauca augaaauucg gaaauguuuc     3180

uacuauaagg auugcuacug caccacgaaa cuggaccaga agucugggca gauguuuaac     3240

cugaccgucc uucccaauga cgcccacucc ccuaaaggaa caaccgaggc ugugaucccc     3300

gugaacaaga aucgcaaaga ugucaacaag uacggcgggu ucagcggccu gcaauauguu     3360

aucguggcca uugagggaaa gaaaaagaga ggcaaaaaga caaaacucgu gaagaagauc     3420

uccggggucc cucugcaccu caaagcggcc ucucuggacg aaaagauuaa auacaucgag     3480

gaaaaggaga aucuuaccga ugugaaaauu aucaaggaca gcauucccgu uaaccagaug     3540

aucgagaugg auggaggcga auaucugcuc acguccccga ucgaguuugu gaaugggcgg     3600

cagcuggucc ugaacgaaaa gcagugugcu cuuauugccg acaucuacaa ugcuauuuau     3660

aaacaagauu gcgacaaccu ggaugacgug cucaugaucc agcuguacau ugagcucauc     3720

aauaagauga aagcccugua uccugccuac cagucuaucg cugaaaaguu cgagagcaug     3780

acagaggauu acguugcggu guccaaagaa gagaaggccg acauuaucaa gcagaugcuu     3840

auuaucaugc aucgcggacc cagaaacggc aaaauucaau augcugauuu uaaugucggg     3900

gaccggaucg gcagaaagaa caaaaugucu cuggaccucg aacgcgugac cuucguuagc     3960

caguccccca caggaaucua caccaagaaa uauaagcugu ga                        4002


<210>  4109
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4109
augggcaagg aguacuaucu ggggcucgac aucggaaccg auagcguggg cugggccguc       60

ugcgacacaa acuacaaaau ucccaaguuc aaagggaaug cuaugugggg aaucagacug      120

uuugaaaagu ccaacucugc cgaggaacgg cgcggcuuca gaagcucccg gcgcagaacg      180

cagcggaaac gcgagagacu uaagcugcuc gaaaugcugu uugaugagga gauuucuaag      240

aucgacccug cguucuuuau uaaacuucgg gaaagcaauc uguaucucga ggauaagucc      300

accaaaaucc cguacugugu uuucucugac gaaaacuaua cagauaagga cuuucaccgc      360

gauuacccca ccaucuauca ucugagaaau gagcugauua aaagcaacaa gccucacgac      420

gugcggcucg uguaccuggc ccuucaucac aucauuaaga aucgcgggca uuuccuguuu      480

gauuacuccu auaaaaacga caagagcgga gauuucaugc ccaucuacaa ugaccucaaa      540

aacuauuuuu acgacaauua ugagauugaa cuggauugcu cugacgucga ugaguucgcu      600

aacacacuua aggacaaauc caagggcaaa accaagaaga augccgaaau cgcugagcug      660

uuuggcguga gcaaaaagua cgauaaaguu cucuauucug cccugucccu gcucagcggg      720

ucuuccguga agcuguucga cauuuucgau gacgaagcuc uuaaagaugu cgagaaaaag      780

agcguguccu uuucuagcgg auucgacgau aacgccaaug acuuucaauc cuaccugggc      840

gagagauucg aacuccugga gaaacuuaag gcgguuuacg acugggccgu gcuggcugau      900

auccucgacg ggaaagaaua uaucagcgag gccaaggucg cuauuuacga aaaacacaag      960

cgggaucuga agacacugaa aaaguauauc aaaauuuaca ugccugagaa gaaagccgag     1020

aucuuuaaca uuucuuccaa ggacaacaau uauguggcgu acagcaagca caucaaaacc     1080

ucuaacggaa cgggcgugcu caucagcacc uguacacagg aagaguucug cgccuaucug     1140

aagaaaaccc uugggauuug uaaggaugcu uccuacgaag agauguuuaa agaaaucgaa     1200

aauucuacau ucaugcccaa gcaggucacc aaagacaacg gaguugugcc uaugcaagug     1260

aaugagaagg aacuggucgc cauucucgau aacgcuaagg aguaccugcc guuucuucug     1320

aauaaagaca acgauggcaa gacgguguau gacaaaauca aggcccucuu cgaauaccgc     1380

auucccuauu acaucggccc ucugaauaca cauagcacca aauccuggcu gguuagaagc     1440

aacgagaaga ucuaucccug gaauauugag gauguggucg acuuugauaa gucugccgaa     1500

aaauucaucg agaaccucac auccaagugc accuaccugc cucaguauga cgugauuccc     1560

aaacacagcc uucuguacaa uaaguuuugu guucucaacg aacugaauaa ccugaaaauc     1620

gacggggaga agauuucugu gcagcuuaag caggauaucu acaacgaccu cuucaaaaag     1680

uauuacaaag ucaccaauaa gaaacugugc gauuaucuga agucccggaa cauugcuuuc     1740

gacgaaauca gcggaaucga uaaugacuuu aaguccucuc ucaaaagcua caaggaucug     1800

guggcguaua accuuacaga cgagcagaaa gaggaaauug ucaaggccau cacgauuuuc     1860

ggcgaugaca aaaagcugcu caaaaagaga cugaagaaug aguuuggggg augucugucc     1920

gaugacgaaa ucaaaaagau uugcaaacuu aaguacaccg gcuggucuaa acucagccgc     1980

gaguuccuga cagaugugac cgcuguuuau aaggaaacag gggagcugau caacaucauu     2040

ugugcccucu gggagaccaa uuacaaccug acgucccuuc uguacagcga cgaauuuugc     2100

ugcgacgaua aggagaauaa aacauucaac gacaagauca augaacucaa cucuuuugga     2160

aaugauaaaa cccugaacca aauuguggag gaacuguaug ucuccccgcu ugugaagcgg     2220

ccuaucuacc agagccuccu gauuaccaaa gagcuggaga agaucaugaa gugucccccu     2280

aaaaagaucu ucguugaagu ggccagaggc gcugaggaaa agaagcgcac agucucucgg     2340

agaaauaaac uccuggaccu uuauaagucc ugcaagaacg auuacccccu gcucuuugac     2400

gagcugcaga acaauaccac agaugacgag cugaaaagcg auaaacuuua ucucuacuuc     2460

acgcaguuug gcaaauguau guauaccggg gaaaacauug agcugggaga ccuguucaau     2520

aagaaccucu acgauaucga ccauauuuac ccccaaucua aaaucaagga cgauagccug     2580

gacaaucgcg ugcuugugaa gaaaacaacc aacgccaaga aagauaauga auauccucug     2640

uccagccaga uucgggagaa gaugacaccg cacuggaaag cgcuccugga caagggccug     2700

aucaacaaaa agaaguacga aagacuuguu cgcaauuauc cccugucuga ugaggagcuc     2760

ucccaguuua ucagccggca acuggucgaa accagacagu ccacgaaagc uguggccaac     2820

auucucgagc agcuguaccc ucgccccgac accgaaaucg uguaugucaa ggccgcucuu     2880

gccucugagu uccggcacca guacgauaug cugaaaugca gagaagugaa ugaccuccau     2940

cacgcuaagg augccuaucu gaacauuguu guggggaaug cguacaacgg aaaauucaca     3000

cauaauaagg ccaacuuuau caagggccug caaaacaaug acaaggcuua caccgucaaa     3060

cuugauagca ugcugaagca caacauugac ggggccugga ucgcugauaa uaacgagucc     3120

cucaauauug ugaagucuac aaugaacaaa aauaacaucc gguauacccg cuacgccuuc     3180

gagaagaagg gcggacuguu uaaaguuacg auccucaaga aaggcaaugg gcaggcgagc     3240

auuaagaaaa acgacaagag aaauuccauc gaaaaguaug gaggcuacaa ccaggugucu     3300

agcacauauu ucuccugugu cgagcauggg aaagacaaua accggaugau uagcauguuu     3360

ucugugaauc uguacgauaa gaacaaauau gaagagaauc cuaucgccua ccuuauugaa     3420

aacuacggac ugaucaaccc caucauuaag guuaaaaagg ugaaaaucca guccugcauu     3480

agcuucgaca auaugcucug ucaccugggc gggaaggauu cuggcggauc ccaaaucagc     3540

uauagaccgg gcguccagcu ggugcuuggg uacgacaacg agcgcuauau ucggaaugug     3600

aucagaugcg cuggaggcga ucugcaccuc cagaccucua agaacgagaa agacaucguc     3660

acagccgaaa agaauauuga gcuguuuaac cuccugaucg agaaaaugaa gaauaacauu     3720

uacgggguuc uuuauuccaa ucugggagau aaacuguacu guagcaagga gaaguucgag     3780

aaacucgaca ucguggaaca gugcaacgug auuaagaaug uccugaccau ccuuaaaaac     3840

aaugcuucca agggcgaucu gaaaagcauu gggaucaagg ccaacggcgc cauccgccuc     3900

aauaacaaca uuucugagau caagaaaguu aagagcauua aacugaucaa ucaauccgug     3960

accggacucu uugaacagga gauugaacug cuuaaccugu ga                        4002


<210>  4110
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4110
augagccaga acaucgugga cuacugcauu ggccuggauc ucgggacagg auccgucggc       60

ugggccguug uggacaugaa ucacagacug augaagcgga acgggaaaca ucuuugggga      120

ucucgccugu ucagcaaugc ugagacagcc gcgacgagac gguccucucg cagcaucaga      180

cggcgcuaua acaagagacg ggaacgcauu agacuccugc gggcuauccu ucaagauaug      240

gugcuggaga aagaccccac cuuuuucauu cgccucgaac acacauccuu ucuggaugag      300

gaagacaagg ccaaauaccu gggcaccgau uauaaggaca auuacaaccu cuucaucgau      360

gaggacuuua augauuauac auacuaucau aaguacccua ccaucuacca ccugagaaaa      420

gcccuuugug agucuacaga aaaggcugac ccgcggcuga uuuaucucgc ccugcaucac      480

aucgucaaau accgcgggaa cuuccuuuau gagggacaga aguuuaauau ggaugcuagc      540

aacauugaag acaaacuguc cgacguguuc acccaguuug ccgauuucaa uaacaucccc      600

uacgaggacg augagaagaa gaaucucgaa auucuggaga uccugaaaaa gccucucagc      660

aaaaaggcga aaguugacga agugauggcc cugauuucuc ccgagaagga auuuaaaucc      720

gcuuauaagg agcuugucac gggcaucgcc ggcaacaaga ugaaugugac caaaaugauc      780

cugugcgaga gcauuaagca gggggauucu gaaaucaaac ucaaguucuc cgacagcaac      840

uacgaugacc aauuuucuga gguugaaaac gaucugggag aguaugugga guucauugac      900

ucccuucaca auaucuacag cugggucgaa cugcagacaa uuaugggcgc uacccauaca      960

gauaacgccu ccaucucuga ggcgauggug agcagauaca auaaacacca ugaagaccuc     1020

cagcugcuga agaaguguau caaagacaac gugccuaaga aauauuucga uauguuucgg     1080

aaugacuccg agaaggucaa aggguacuau aacuacauua auagacccaa caaggccccu     1140

guugaugagu ucuauaaguu ugugaaaaag ugcaucgaaa aaguggacac cccggaagcu     1200

aagcagauuc uccacgauau cgagcuggag aauuuccuuc ugaaacaaaa cagccgcacg     1260

aauggaucug uccccuacca gaugcagcuc gacgaaauga uuaagaucau cgauaaccag     1320

gccaaguauu acccugugcu gaaagagaag cgggaacaac uucuguccau ucucacauuu     1380

agaauccccu acuauuucgg cccucugaau gagaccagcg agcacgcuug gauuaaacgc     1440

cuggaaggga aggagaacca gcggauccuc cccuggaauu accaggacac aguugaugug     1500

gacgccaccg ccgaaggauu uauuaaaaga augcgcucuu auuguaccua cuucccggau     1560

gaggaagucc ugccuaagaa cagccuuauc guguccaaau augagguuua caacgagcug     1620

aauaagauuc ggguggacga uaagcuccug gaagucgacg ugaaaaacga caucuauaau     1680

gagcuguuua ugaagaacaa aacagucacg gaaaagaaac uuaagaauug gcucgugaac     1740

aaucaaugcu guagaaagga ugcugagauc aaaggcuucc agaaggaaaa ccaguuuucu     1800

accagccuga cacccuggau ugacuucacc aauaucuuug gcaaaauuga ucaguccaac     1860

uucgaccuga ucgagaagau uaucuacgau cucacaguuu ucgaggacaa aaagaucaug     1920

aagcgccggc ugaaaaagaa auacgcgcuu ccugaugaca agauuaaaca aauccugaag     1980

cucaaguaua aagauuggag cagacugucu aagaaacugc uugacgggau uguggccgau     2040

aaucgcuuug gauccagcgu caccgugcuc gacguucugg aaaugucucg gcugaaccuc     2100

auggagauca uuaaugauaa ggaacugggc uacgcucaga ugaucgagga agccuccagc     2160

ugccccaaag acgggaaguu cacguaugag gagguggcca aacuugcugg aucccccgcc     2220

cugaagagag gcaucuggca gucucuccag auugucgaag agaucacaaa ggugaugaaa     2280

ugucggccua aguacauuua uaucgaauuu gagcgcagcg aggaagcgaa agagagaacc     2340

gaauccaaga uuaaaaagcu ggagaacgug uacaaggacc uggaugaaca aaccaaaaag     2400

gaguauaaau cuguucuuga ggaacucaag ggguucgaca auacaaagaa gaucagcucc     2460

gauagccugu uucuguacuu cacccagcuc ggaaagugca uguacucugg caaaaagcug     2520

gacaucgauu cccuugacaa auaucagauu gaucauaucg ucccgcagag ccuggugaag     2580

gacgauucuu uugacaaccg ggugcucguc cugcccuccg agaaccaaag aaaacuggau     2640

gaccuugugg uuccuuucga cauucgcgau aagauguacc gguuuuggaa gcuccuguuc     2700

gaccacgaac ugaucagccc caaaaaguuu uauucucuca uuaaaacaga guacacggaa     2760

agagaugagg agcgcuucau caauagacag cugguggaaa cccggcagau uacaaagaac     2820

gucacccaga ucaucgagga ccauuauagc acaaccaaag uggcugccau ucgcgccaau     2880

cuuucccacg aauuuagagu uaagaaccau aucuacaaga aucgggauau uaacgacuau     2940

caccacgcuc augaugccua caucguggcu cugauuggcg gguucaugcg cgacagauac     3000

ccuaauaugc acgauagcaa agccgucuau ucugaguaca ugaagauguu ccggaaaaac     3060

aagaaugacc agaaaaagug gaaagaugga uuugugauca acuccaugaa uuaucccuac     3120

gaaguugacg gcaagcucau cuggaacccg gaucugauua augagaucaa gaaauguuuc     3180

uauuacaagg acugcuauug cacgaccaaa cuggaccaaa agagcgggca gauguuuaac     3240

cuuacagugc ugccuaauga ugcgcauucc gccaaaggaa ccacagaggc ugucauuccc     3300

gugaacaaga accgcaagga cgugaauaaa uacggcgggu ucucuggccu ccaauacguc     3360

aucgccgcua uugaaggaac caagaaaaag ggcaaaaagc ugguuaaggu gagaaaacuc     3420

agcgggaucc cucuguaucu uaagcaggcc gauaucaaag agcagauuga auacguggag     3480

aaggaggaaa aacuguccga cgucaagauc auuaagaaca auaucccccu caaccagcug     3540

auugagaucg auggacggca auaucugcuu acgucuccua cagaaugugu gaaugcgaug     3600

cagcugguuc ucaacgagga acagugcaaa cugauugccg acaucuacaa ugcuaucuau     3660

aagcaggauu uugacggccu cgauaacaug cugaugauuc aacuuuaccu gcagcucauc     3720

gacaaacuga agacccugua ucccauuuac auggggaucg uggagaaauu cgagaaacuu     3780

acagaagauu uugucagcau uuccaaagag gaaaaggcca augugaucaa gcagaugcug     3840

aucauuaugc acaaaggacc gcagaacggc aauaucauuu acgacgauuu caacguuggg     3900

aagcgcaucg gcagacucaa uggacggacc uuuuaucugg acaacauuga guucaucucu     3960

caaagcccua ccggcaucua caccaaaaag uauaaacucu ga                        4002


<210>  4111
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4111
auguacaccu auuacuucaa ggagagcgaa augguguaug acgucggccu ggauaucggg       60

acaggauccg uuggcugggu ggcccucgac gagaacggga aacuggcuag agccaaggga      120

aagaaucuug ugggcguccg gcuguuugau acggcgcaga ccgcugccga ccgcagaggg      180

uuccggacaa cccgcagacg gcucucucgc agaaaguggc ggcugcgccu ucuggaugaa      240

cucuuuagcg ccgagauuaa cgaaaucgac uccucuuucu uucaaagacu gaaauacagc      300

uaugugcacc ccaaggauga ggagaauaag gcucauuacu auggaggcua ccuguucccu      360

acagaagagg aaaccaaaaa guuucaccgg uccuacccga caauuuauca ucuccgccag      420

gagcugaugg cccagcccaa caaaagauuc gacauccggg agauuuaccu ugcuauccac      480

caucugguua aguaucgcgg ccacuuucuc ucuagccagg aaaagaucac cauugggucc      540

acguacaauc cugaggaucu ggccaacgcg aucgaagugu augccgacga gaagggacuu      600

agcugggaac ugaauaaccc cgagcaacuc accgagauua ucucuggcga agcuggguac      660

ggacugaaua aguccaugaa agccgaugag gcucugaaac ucuucgaauu ugacaacaau      720

caggauaaag ucgccauuaa gacacugcuu gcgggccuga ccgggaacca gaucgacuuc      780

gccaaacucu uuggaaagga cauuagcgau aaagacgagg cuaagcugug gaaacuuaaa      840

cuggaugacg aagcccucga ggagaagucu cagacaaucc ugucccaacu gaccgaugaa      900

gagaucgaac ucuuccacgc ugugguucag gccuaugacg gcuucgugcu gauuggccuu      960

cugaaugggg ccgauagcgu cucugcugcg auggugcagc ucuacgacca gcauagagag     1020

gaucggaaac ugcuuaaguc ccuggcccaa aaagcuggac ucaagcacaa gagauuuagc     1080

gaaaucuacg agcagcuggc ccuggccacg gacgaggcua caauuaaaaa cggcaucucc     1140

accgcccgcg aacucgugga ggaaucuaau cugagcaagg aggucaaaga ggauacacuu     1200

cggagacugg acgaaaacga guuccucccu aagcagcgca ccaaagcgaa cuccguuauu     1260

ccccaucaac ugcaccuggc ugaacuucag aagauccucc agaaucaggg gcaauauuac     1320

ccuuuucugc uggacaccuu cgagaaggaa gauggacagg acaacaaaau cgaggagcuc     1380

cugcgguuuc gcauuccgua uuacgugggc ccccuuguga caaagaaaga ugucgaacac     1440

gccgggggag acgccgauaa ucauugggug gagagaaacg aaggcuucga aaagagccgg     1500

guuacgccuu ggaauuuuga caaaguguuc aacagagaua aggcugcccg cgacuuuauc     1560

gaacggcuga ccggcaauga uacauaucuc auuggggaga agacccugcc ccagaacucu     1620

cugagauacc agcuuuucac aguccucaau gagcugaaca augugcgcgu uaacggcaaa     1680

aaguuugacu ccaaaaccaa ggcugaucug aucaaugacc ucuucaaagc ccggaagacg     1740

gugagccugu cugcgcuuaa agauuaucug aaggcccaag gaaaggggga cgucacaauu     1800

accggacucg cugacgaaag caaauuuaac uccucucuga gcuccuacaa ugaucugaag     1860

aaaaccuucg acgccgagua ccuugaaaac gaggauaacc aggaaacacu cgagaagauc     1920

aucgagauuc agaccguguu cgaagacagc aaaaucgcuu cuagagagcu guccaagcug     1980

ccucucgaug acgaucaggu caagaaacug agccaaacac acuauacggg cugggggcgg     2040

cuuucugaaa agcugcucga cuccaaaauu aucgaugagc gcggccagaa ggugagcauu     2100

cuggacaaac ugaaguccac cucucagaau uuuaugagca ucauuaacaa ugauaaguac     2160

ggaguucagg ccuggaucac agagcaaaac accggcuccu cuaaacuuac auucgacgaa     2220

aaggugaaug agcucaccac gagccccgcg aacaaaagag ggaucaagca guccuuugcc     2280

guccugaaug auauuaaaaa ggcuaugaag gaggagccgc ggagagugua ucuggaguuc     2340

gcccgcgaag accagaccag cguucggucu gugccuagau acaaccaacu caaagagaag     2400

uaucagucca aaagccuguc ugaagaggcu aagguccuua aaaagacacu ggacggaaau     2460

aaaaacaaga uguccgauga ccgcuacuuu cucuauuucc agcagcaagg caaggauaug     2520

uacaccggga gacccaucaa uuuugaacgg cugagccagg acuacgauau ugaccauauc     2580

auuccucagg ccuucacaaa agaugacucu cuggauaacc gcgugcuugu gagcagaccc     2640

gagaaugccc ggaaguccga cagcuuugcu uauaccgaug aaguucagaa acaagacgga     2700

ucucugugga cgucccuccu caagagcggc uucaucaacc gcaaaaagua cgagagacug     2760

acaaaggcgg ggaaauaucu ggacggccag aagaccggau uuaucgcccg gcagcuuguc     2820

gagacacgcc agauuaucaa aaauguggcu ucccugauug aaggcgagua cgaaaacucu     2880

aaggccgugg ccaucagaag cgagauuacc gcugauaugc ggcuccuggu cgggaucaaa     2940

aagcaccgcg aaaucaacuc cuuccaucac gccuuugacg cgcugcuuau uaccgcugcc     3000

ggacaauaua ugcagaauag auaccccgau cgggacucua caaacgugua uaaugaguuc     3060

gauagauaca cgaacgacua ccugaagaau cuccgccagc ugagcucccg ggaugagguu     3120

agacgccuca aaucuuucgg cuuugugguc gggaccaugc ggaagggaaa cgaagacugg     3180

agcgaggaaa auacauccua ucugagaaaa gugaugaugu ucaagaacau ccuuaccacc     3240

aaaaagacag agaaggaucg cggcccucug aauaaagaga cgauuuuuag cccgaagucu     3300

gggaaaaagc ucaucccccu gaacuccaaa cggagcgaca cagcccugua cggcggauau     3360

ucuaauguuu acuccgcuua uaugacccuu gugagagcca acggcaagaa ucugcucauu     3420

aagaucccua ucagcauugc gaaccaaauc gaagucggga aucugaaaau uaacgauuac     3480

aucgugaaca aucccgcuau uaagaaauuc gagaagaucc ucauuucuaa acugccucuu     3540

ggacagcugg uuaacgaaga cggcaaucug aucuaucucg ccuccaacga guaccgccac     3600

aaugccaagc agcuguggcu uagcaccaca gaugcugaca aaaucgccuc cauuagcgaa     3660

aacucuagcg acgaggagcu gcucgaggcu uacgauaucc ugaccuccga aaaugugaag     3720

aaccgguuuc ccuucuuuaa gaaagacauu gauaagcucu cucaggucag agacgaguuc     3780

cuggauagcg acaaacggau cgccgugauu caaacaaucc uucgcgggcu gcagaucgau     3840

gcggccuauc aggcuccggu gaagauuauc uccaaaaagg ucucugacug gcauaagcug     3900

cagcaaagcg gaggcauuaa acucuccgau aauucugaaa ugaucuacca gagcgccacg     3960

gggauuuuug aaaccagagu uaagaucucc gaccugcuuu ga                        4002


<210>  4112
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4112
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacgaacc aguaugaucu gguuaaaaga aagaugaaag uggcuggcaa uuccgacaag      120

aaacaaauca agaagaacuu cuggggagug cggcuuuuug augacgggca gaccgccguc      180

gaucgcagaa ugaaucggac agcgcgcaga cggauugagc gcagacggaa ccgcaucucu      240

uaccugcagg aaauuuucgc uguggagaug gccaauaucg acgccaacuu uuucugcaga      300

cucaaugaua gcuuuuaugu ugacuccgaa aaacggaacu cucgccaccc uuucuuugcu      360

accaucgagg aagagguggc cuaccauaag aauuauagaa caauuuacca ucugcgggag      420

gaacuuguca acagcuccga gaaagcugau cugcgccucg uguaucuggc ccuggcgcac      480

aucauuaagu acagaggcaa uuuccucauc gaaggggccc uggacaccaa aaacacaucu      540

guugauggag uguacgagca guuuauucaa accuauaauc aggucuucau gagcaacauc      600

gaggaaggca cgcuugcuaa gguggaggaa aacauugagg uggccaauau ccuggcuggc      660

aaguuuaccc ggagagaaaa auucgagcgc auccuccagc uguacccggg ggagaagucc      720

acaggaaugu uugcccaguu cauuagccuu aucgucggcu cuaaagggaa cuuccaaaag      780

guuuuugacc ugauugaaaa gaccgauauc gagugugcga aggacuccua ugaagaggac      840

cucgaagccc ugcuggcuau uaucggagau gaguacgccg aacucuucgu ggcugccaaa      900

aauacauaua acgccguggu ccugagcucu aucauuaccg ugacggcuac agaaaccaau      960

gcgaagcucu ccgccagcau gaucgagcgg uuugacgcuc acgaaaagga ucugggcgag     1020

cucaaagccu ucauuaagcu gcaucuuccc aaacaguacc aggaaaucuu uaacaaugcc     1080

gcuauugacg gguaugccgg auacaucgau ggcaagacaa aacagguuga cuucuacaag     1140

uaucugaaga ccauccucga gaacauugag ggcgcggauu acuuuaucgc uaaaauugaa     1200

gaggaaaauu uccugagaaa gcaacgcacc uuugacaacg gggccauccc ucaccagcug     1260

caccucgagg agcuggaagc cauuauccau cagcaagcua aauauuaccc cuuccuucgg     1320

gaggauuaug aaaagauuaa aucucuggug acauuuagaa ucccuuacuu cgucggaccc     1380

cucgccaagg gccaguccga guuugcuugg cugacgcgca aggccgacgg ggaaauccgg     1440

ccuuggaaua uugaggagaa aguggauuuc ggaaagagcg cgguugacuu caucgagaaa     1500

augaccaaca aggacacaua ucuuccgaaa gagaaugugc ugcccaagca cucccucugc     1560

uaccagaagu acauggucua uaacgaacug accaaaguga gauacauuga ugaccagggc     1620

aagacaaauu auuuuucugg gcaagagaaa cagcagaucu ucaacgaucu guuuaagcag     1680

aaacgcaagg ucaaaaagaa ggaccucgaa cuguuccuuc ggaacauuaa ucauaucgag     1740

agcccuacca ucgagggacu ggaagauucc uuuaacgcca gcuacgcuac guaucacgac     1800

cuccugaaag ugggcaugaa gcaagagauu cuggauaauc cccuuaacac agaaaugcuc     1860

gaggacaucg uuaaaauucu gaccguguuc gaagauaagc cuaugaucaa agagcagcug     1920

cagcaguuuu cugacguccu cgauggcggg gugcugaaga aacuugagag acgccacuac     1980

accggauggg gccggcuguc cgccaaacuc cugguuggga uuagagaaaa gcaaagccau     2040

cugacaaucc uugacuaucu caugaaugau gacggacuga accggaaucu gaugcagcuc     2100

aucaacgacu cuaaucugag cuucaaaucc auuaucgaga aggaacaggu gucuaccaca     2160

gauaaagacc uucagagcau ugucgcugau cuggccggcu cccccgcgau caagaagggg     2220

auucuccaaa gccugaaaau cguggacgag cuggugucua ucaugggcua cccgccucag     2280

acgauuguug ucgagauggc ccgcgaaaac cagaccacag gaaagggcaa aaauaacucc     2340

aagcccagau acaaaagccu ugagaaggcu aucaaggaau uugggucuaa aauucucaag     2400

gagcacccua ccgauaauca agaacugaaa aacaaucggc uguaucucua cuaucugcag     2460

aacggaaagg acauguacac aggccaggag cuugauaucc auaaccuguc caauuaugac     2520

auugaucaca ucgugcccca gagcuucauu accgacaacu ccaucgauaa ucucgugcug     2580

acgucuagcg ccgggaacag agagaaagga ggcgacguuc ccccucugga aaucgugcgc     2640

aagcggaaag ucuuuuggga gaaacuuuac caagggaauc ucauguccaa gagaaaguuc     2700

gauuaucuga ccaaggcuga acgcggcgga cugacagacg ccgacaaagc cagauuuauu     2760

caucggcagc ucguggagac ccgccagauc acaaagaacg ucgcuaauau ucugcaccag     2820

agauucaaca augaaaccga uaaccacggc aauacgaugg agcaagugcg gaucguuaca     2880

cuuaaaucug cgcuggugag ccaguuccgc aagcaguuuc agcucuacaa ggucagagag     2940

gugaacgacu accaucacgc ccaugaugcu uaucugaaug ggguuguggc caacacccug     3000

cuuaaagucu acccgcaacu ggaacccgag uucguguaug gagaauacca ccaguuugac     3060

ugguucaagg ccaauaaagc uacagccaag aaacaguuuu auaccaacau uaugcuuuuc     3120

uuuggccaaa aggagcggau caucgaugaa aacggggaga uucuguggga caagaaauac     3180

cucgagacca ucaagaaagu gcuggauuau cgccagauga auauugucaa gaaaacagaa     3240

auccagaagg gagaguucuc caaggcgacg auuaaaccua agggcaacag cucuaaacuu     3300

auccccagaa aggaaaauug ggacccuaug aaauacgggg gccuggauuc ccccaacaug     3360

gcuuacgccg uuaucauuga gcacgccaag ggaaagaaaa agcucaucuu ugagaaaaag     3420

auuauccgga uuaccaucau ggaacgcaaa auguucgaga aggacgaaga ggcuuuucug     3480

gaagagaaag gcuauagaca uccgaaggug cugacaaaac uuccuaagua cacccuguau     3540

gagugugaaa aggggcggag acgcaugcuc gccagcgcga augaggcuca gaaaggaaac     3600

caacuggugc ucucuaauca ccuggucucc cuucuguacc augccaagaa cugcgaagcc     3660

agcgauggca aaucucucaa guauauugag gcucaccggg aaacauucag cgagcugcug     3720

gcccaggugu ccgaguucgc uaccagauac acgcuugccg acgcgaaucu gagcaagauc     3780

aacaaucucu uugaacagaa caaagagggg gacauccagg ccauugcuca aucuuucguu     3840

gaucugaugg ccuuuaaugc uaugggagcc cccgccuccu ucaaguuuuu cgaagcuaca     3900

aucgaccgca aacgguauac caaccucaag gagcugcuua gcuccaccau uaucuaccag     3960

ucuauuacag gccuguacga aagcagaaaa cgccuggaug acuga                     4005


<210>  4113
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4113
augaagaaac ccuacaccau cggccuggac auugggacaa acagcguggg augggccgcu       60

cucacggauc aguaugaccu ggucaagaga aaaaugaagg uugccggcaa uuccgagaaa      120

aagcaaauca agaaaaaccu uuggggagug cggcuggugg augaagggaa gaccgcggcu      180

caccgcagag ucaaucggac aacccgcaga cggauugagc gcagacggaa ccgcaucucu      240

uaccuccagg aaauuuucac agccgagaug uuugaagugg acgccaauuu cuuuuauaga      300

cuggaggaua gcuucuacau cgaguccgaa aaacggcagu cucgccaucc uuucuucgcu      360

accaucgagg aagagguugc cuaucacgag aacuacagaa caauuuauca ucuucgggaa      420

aagcugguga auagcuccga caaagcugau cuccgccugg ucuaccuggc ccucgcgcac      480

aucauuaagu acagaggcaa cuuucugauc gaggggaagc ucgacaccaa aaauacgucu      540

guggaugaag uuuucaagca guuuauuaaa accuauaacc aaguguucgc cagcgacauc      600

gaggaaggau cccugacacg gauugaggag aauaacgaag ucgcuaagau cuuuagcgag      660

aaacucacca agagagaaaa acuggauaag auccuuaauc uguacccgaa cgagaagucu      720

acagaccucu ucgcccaguu uauuucccug aucauuggca gcaaaggcaa uuucaagaaa      780

uucuuuaacc ugaccgaaaa gacggauauc gagugcgcua aagacucuua ugaggaagac      840

cucgaggugc ugcuugcccg cgugggggau gaauacgcgg agauuuucgu cgccgcuaag      900

aacgccuaua augcuguugu gcuguccagc aucaucacag ugucuaacac cgaaacaaag      960

gccaaacucu ccgccagcau gauugagcgg uuugacaagc augauaaaga ccugaagaga     1020

augaaggcuu ucuuuaaggu ccgccuuccc gagaauuuca acgaaguguu uaaugauguu     1080

gagaaggacg gauacgcggg cuauaucgaa gggaaaacca agcaggaggc cuucuacaaa     1140

uacaugaaga aaaugcugga gcacguggaa ggagcugauu auuuuauuaa ccagaucgag     1200

gaagagaauu uccuccggaa gcaaagaacc uuugacaacg gcgccauucc ucaccagcug     1260

caucuggagg aacucgaggc cauccugcac cagcaggcua aguacuaucc cuuccuuaaa     1320

gucgauuacg aaaagaucaa aucccuggug acauuucgca uuccuuauuu cguugggccc     1380

cucgccaaug gacaaucuga guucagcugg cugacgcgga aggcggacgg cgaaaucaga     1440

ccuuggaaca uugaggaaaa aguggauuuu ggcaaguccg cuaucgacuu cauugagaaa     1500

augaccaaua aggacacaua ccuuccgaag gagaacgucc ugcccaaaca uagcaugugu     1560

uaucagaagu acauggugua caaugaacuc accaaaaucc gcuauacaga ugaccagggg     1620

aagacccacu acuuuucugg acaagagaaa cagcagauuu ucaacgaucu guuuaagcag     1680

aagcggaaag ucaagaaaaa ggaccuggaa cucuuccugu auaauaugaa ccacguggag     1740

uccccuacgg uugaaggcgu ggaggaugcc uuuaacagcu cuuucacaac cuaccaugac     1800

cuucaaaaag ucggggugcc ccaggagauc cuggaugacc cucucaauac cgaaaugcug     1860

gaggaaauca uuaagauccu gacaguuuuu gaggauaaga gaaugauuaa cgagcgccuu     1920

caggaguuca gcaaugugcu cgacgaagcc guccugaaaa agcuggaacg gagacacuau     1980

accggauggg gccggcucuc cgcuaaacug cuuaucggga uucgcgauaa ggagucucau     2040

cugacaaucc ucgacuaccu gaugaacgau gacaaacaca auagaaaccu gaugcagcuu     2100

aucaaugaca gcaaccucuc cuuuaagagc auuaucgaaa aggagcaagu gucuacggcc     2160

gauaaagaca uucaguccau cguggcugau cuggccggaa gccccgcgau uaagaaaggc     2220

auccugcagu cucucaagau cguugacgag cuggucggca uuauggggua uccgccucag     2280

accaucgugg uggaaauggc ccgggagaau caaacaaccg gaaaaggcaa gaacaauucc     2340

aaacccagau ucacaagccu ugaaaaggcu auuaaggagc ugggguccca gauccucaaa     2400

gaacauccua ccgauaacca gggacugaag aaugaccgcc uguaccuuua cuaucuccag     2460

aacggcaaag auauguacac ggggcaagag cuggacauuc acaaucuguc uaacuaugau     2520

aucgaccacg ucgugcccca gagcuuuauc accgauaacu ccauugacaa ucggguucuc     2580

gccucuagcg cugccaacag agagaagggc gauaaugugc ccucccugga agucgugcgc     2640

aaaagaaagg uuuacuggga gaaacuuuau caggcgaaac ugaugagcaa gcggaaauuc     2700

gacuaccuca caaaggccga acgcggaggc cugaccgagg cugacaaagc cagauucauc     2760

caucggcaac ugguggaaac acgccagauu accaagaacg ucgcuaauau ccuucaccag     2820

agauuuaacu gcaagaaaga ugagucuggg aaugugauug agcagguucg gaucgugacg     2880

cucaaggccg cccuggucuc ccaauuccgc aaacaguuuc agcuguauaa ggugagagaa     2940

gugaacgacu accaucacgc ucacgaugcg uaccucaauu gugucguugc caacacacug     3000

cuuaaagugu auccucagcu ggagccggag uucguguacg gagacuauca ucaauuugau     3060

ugguucaagg cuaauaaggc caccgccaaa aagcaguuuu acacaaacau uaugcuuuuc     3120

uuugcuaaaa aggaccggau caucgaugaa aauggcgaga uucuguggga caaaaaguau     3180

cuggauacca ucaagaaagu ccuuaacuac cgccagauga auauugugaa gaaaaccgaa     3240

auccagaagg gggaguucag caacgccaca gcgaacccca aaggaaauuc uuccaagcug     3300

auuccuagaa aagcugacug ggaucccauc aaguauggcg gguuugacgg cagcaacaug     3360

gccuacgcca ucguuauuga acacgagaag cggaaaaaga aaacggugau caagaaagaa     3420

cucauucaaa ucaauauuau ggagagaacc gcuuucgaga aggaccagaa ggaguuccug     3480

gaaggaaaag gcuaccgcaa cccuaagguc aucacaaaaa uccccaagua uacccucuac     3540

gaaugcgaga augggcggag acgcaugcug ggaucugcca acgaagcgca gaaaggcaau     3600

caaauggugc uuccgaacca ucugaugaca cuccuguauc acgcuaagaa uugugaggcc     3660

agcgauggga agucccuggc cuacauugag agccaucggg aaauguuugc ugagcuucug     3720

gacucuaucu ccgaauuugc cagcagauau acccucgcug augccaaccu ggagaaaauu     3780

aauacgaucu uugaacagaa caaguccgga gacguuaaag ugauugcgca gucuuucguc     3840

aaucuccugg aguuuaacgc caugggcgcu ccugccagcu ucaaguacuu ugagacaaau     3900

aucgaacgca aacgguauaa caaucuuaag gagcugcuca acgcuaccau uaucuaccag     3960

uccaucaccg ggcuguacga agccagaaag cgccuggaug acuga                     4005


<210>  4114
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4114
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacggauc aguaugaccu gguuaaaaga aagaugaaag uggcuggcaa cuccgauaag      120

aaacaaauca agaagaauuu cuggggagug cggcuuuuug acgauggaca gaccgccguc      180

gaccgcagaa ugaaccggac agcgcgcaga cggauugagc gcagacggaa ucgcaucucu      240

uaccugcagg aaauuuucgc uguggagaug gccaacaucg augccaacuu uuucugcaga      300

cucaaugaca gcuuuuaugu ugauuccgaa aaacgcaauu cucggcaccc uuucuuugcu      360

accaucgagg aagagguggc cuaccauaag aacuauagaa caauuuacca ucugcgggag      420

gaacuuguca auagcuccga gaaagcugac cugcgccucg uguaucuggc ccuggcgcac      480

aucauuaagu acagaggcaa cuuccucauc gaaggggccc uggauaccaa aaauacaucu      540

guugacgagg uguacaagca guuuauucaa accuauaacc aggucuucau gagcaauauc      600

gaggaaggag cucuugccaa gguggaggaa aacacggagg uggcuuccau ucuggccggc      660

aaauuuaccc ggagagaaaa guucgagcgc auccuccggc uguacccggg cgagaaaagc      720

acagggaugu uugcgcaguu caucucucuu auugucggaa acaagggcaa uuuccagaaa      780

guuuuuaacc ugguggaaaa gaccgauauc gagugugcca aagacuccua ugaagaggac      840

cucgaagcuc ugcuggccau uaucggggau gaguacgcug aacucuucgu ggccgccaag      900

aauacauaua acgcugucgu gcugagcucu auuaucaccg uuacggcgac agaaaccaau      960

gccaaacuuu ccgcuagcau gaucgagaga uuugacgccc acgaaaaaga gcugggagaa     1020

cucaaggccu ucauuaaacu gcaucuuccc aagcaauacc aggagaucuu uaacaaugcu     1080

gagauugaug gcuaugccgg guacaucgac ggaaaaacaa aacaggugga uuucuacaag     1140

uaucugaaga ccacccucga aaacgucgag ggcgcggacu acuuuauuac aaagaucgaa     1200

gaggagaauu uccugcgcaa acagcggacg uuugauaacg gcgugauccc ucaccaacug     1260

caccucgagg agcuggaagc uauucuucau cagcaggcca aguauuaccc cuuccugaga     1320

gagggguaug acaaaaucaa gucucucguu accuuucgca uuccuuacuu cgugggaccc     1380

cuggccaaug gccaauccga auuugcuugg cuuacacgga aggaugacgg ggagaucaga     1440

ccuuggaaca uugaggaaaa agucgauuuc ggaaagagcg ccguggacuu caucgagaaa     1500

augaccaaua aggacacaua ucugccgaaa gaaaacgucc uccccaagca cucccugugc     1560

uaccagaagu acauggugua uaaugagcug accaaaauuc gcuacaucga ugaccagggc     1620

aagacgaacu auuuuucugg gcgggaaaaa cagcaaaucu ucaacgaucu cuuuaagcag     1680

aaaagaaagg uuaaaaagaa ggaccuggag cuuuuccugc gcaauauuaa ccauaucgag     1740

agcccuacaa uugaaggacu cgaggauucc uuuaaugcua gcuacgccac cuaucacgac     1800

cugcugaaag ugggccuuaa gcaggaaauc cucgauaacc cccugaauac cgagauucug     1860

gaagacaucg ucaaaauccu cacaguguuc gaggauaagc ggaugauuaa agagcagcug     1920

gaacaauuuu cugacguucu ugauggcgug guccugaaga agcucgagag acggcacuac     1980

accggguggg gacgccuguc cgcgaaacug cuugugggca ucagagacaa gcagagccau     2040

cucacaauuc uggauuaucu gaugaacgac gacgggcuca aucggaaccu gaugcagcuu     2100

aucaaugauu cuaaccugag cuucaaaucc auuaucgaaa aggaacaggu gucuacgacc     2160

gacaaagauc uccaaagcau cguugccgac cuggcuggau ccccugccau uaagaagggc     2220

auccugcaga gccuuaaaau ugucgaugag cucgugucua ucauggggua ucccccgcag     2280

acaauugugg ucgaaauggc uagagagaau cagaccacag gcaaggggaa aaacaauucc     2340

aagccucgcu acaaaagccu ggaaaaggcc aucaaggacu uuggcucuca aauccugaaa     2400

gagcacccca ccgauaacca ggaacucaag aauaaccggc uguaccuuua cuaucugcag     2460

aacgggaaag acauuuacac gggacaagag cucgauaucc auaaucuguc caacuaugac     2520

auugaucaca ucgugccuca gagcuucauu accgacaauu ccaucgauaa ccugguucuu     2580

acaucuagcg cgggcaauag agagaaaggg ggagacgugc cuccccucga aauuguccgc     2640

aagagaaagg uguuuuggga gaaacuguac cagggcaacc ugauguccaa gcggaaguuc     2700

gacuaucuca ccaaagccga acgcgggggc cugacagagg cugauaaggc cagauuuauc     2760

caucggcagc uuguugaaac ccgccaaauc acgaaaaaug uggcuaacau ucugcaccag     2820

agauucaaua aggagacaga caaccacgga aauaccaugg agcagguccg gaucgugaca     2880

cucaaaucug cccugguuag ccaguuccgc aagcaauuuc agcuguacaa ggugagagaa     2940

gucaacgauu accaucacgc ccaugacgcu uaucuuaaug gcgugguggc gaacacccug     3000

cucaaagucu acccccagcu ggagccggag uucguuuaug gggaguacca ccaguuugau     3060

ugguucaagg ccaauaaagc uaccgccaag aaacaauuuu auacaaacau uaugcuguuc     3120

uuugcccaga aggaacggau cauugacgag aacggagaga uccucuggga uaagaaauac     3180

cuugaaacga ucaagaaagu gcuggacuau cgccagauua auaucgugaa gaaaaccgag     3240

auucaaaagg gcgaguucuc caaggcuaca aucaaaccca aggggaacag cucuaaacuc     3300

auuccuagaa aggaaaauug ggaucccguc aaauacggag gccuggacuc cccuaacaug     3360

gccuacgcgg ugaucaucga gcacgcuaag gggaagaaaa agauuguuau cgaaaagaag     3420

cugauucaga ucaauauuau ggagcggaaa auguuugaaa aggaugagga agccuuccuu     3480

gaggagaaag gcuaucauca gcccaaggug cugaccaagc ucccgaaaua cacacuguau     3540

gaaugugaga agggaagacg ccggaugcuc agcucugcca acgaagcuca gaaaggcaau     3600

caacuggucc uuuccaacca ccuggugagc cuccuguacc augccaagaa uugcgaggcg     3660

ucugacggga aaagccugaa guauaucgaa gcucacagag agaccuuuuc cgagcuucug     3720

gcccagguua gcgaguucgc cacgaaguac acacucgcug acgccaaccu gucuaaaauu     3780

aauaaccucu ucgaacagaa uaaggaagga gauaucaaag cuaucgccca guccuuugug     3840

gaccugaugg cguucaacgc caugggcgcu ccugccagcu uuaaguucuu ugaggcuacc     3900

auugaucgca aacgguauac caaucuuaag gaacugcugu ccucuacaau cauuuaccaa     3960

agcaucaccg ggcucuacga guccagaaag cgccuggacg auuga                     4005


<210>  4115
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4115
augggcagaa agcccuacau ccugagccuc gacauuggga ccggauccgu gggcuaugcc       60

ugcauggaua aaggguucaa cguccugaag uaccacgaca aagaugcucu uggaguuuau      120

cuguuugacg gcgcccucac agcgcaggag cggcgccaau ucagaacguc ucggcgcaga      180

aagaaucggc gcaucaaaag acuggggcuu cugcaggaac uccuggcucc ucuggugcag      240

aacccgaauu uuuaccaguu ccaacggcag uuugccugga agaacgauaa uauggacuuc      300

aagaacaaaa gccucuccga ggugcugucu uuucuuggau augaaagcaa gaaauacccc      360

accauuuauc aucugcagga ggcccuccug cuuaaggaug aaaaauucga cccugagcug      420

aucuacaugg cucucuacca ccuggucaag uaucgcggcc auuuucuguu cgaucaccuc      480

aagauugaga aucugacaaa caaugacaac augcaugauu uuguggaacu uaucgagacc      540

uacgaaaauc ugaacaauau caaacucaac cuggacuaug agaagacaaa aguuauuuac      600

gagauccuua aggauaauga aaugaccaaa aacgacagag ccaagcgggu gaaaaacaug      660

cagaagaagc uggagcaauu cuccauuaug cuccugggcc ugaaauuuaa ugaagggaag      720

cuguucaacc acgcugacaa ugccgaggaa cugaaaggag cgaaccaguc ucacacauuc      780

gccgauaauu augaggagaa ccuuaccccc uuucugacgg ucgaacagag cgaguucauc      840

gaacgcgcua auaagauuua ccucucccug acccuucagg acauccugaa aggcaaaaag      900

agcauggcca ugucuaaggu ggcugccuau gauaaguuua gaaacgagcu caaacaaguu      960

aaggacauug uguacaaagc ggauuccaca cggacccagu ucaagaagau cuuugucagc     1020

ucuaaaaagu cccugaaaca guacgacgcc acaccuaaug aucaaaccuu cagcucucug     1080

ugucucuuug accaguaucu gauccgcccc aagaaacagu acucccuucu gauuaaggaa     1140

cucaagaaaa ucauuccuca ggauagcgag cuguauuucg aggcugaaaa cgacacgcug     1200

cuuaaggugc ucaauacaac cgauaacgcc uccaucccga ugcaaauuaa ucuguacgag     1260

gcugaaacaa uccugagaaa ccagcagaaa uaucaugccg agaucaccga cgaaaugauu     1320

gagaaggugc ucucucugau ccaguuucgg auucccuacu augucgggcc ucuuguuaau     1380

gaccacaccg ccagcaaauu cggauggaug gagagaaagu ccaacgaaag caucaaaccc     1440

uggaacuuug augagguggu ggaccgcucu aaguccgcua cacaauucau ucggagaaug     1500

acgaauaagu gcagcuaccu gaucaacgaa gauguccucc cuaaaaauuc ucugcuguac     1560

caggagaugg aggugcuuaa cgaacucaau gcgacccaga uccgccugca gacagacccc     1620

aagaaccgga aauauagaau gaugccgcaa auuaagcugu uugccguuga gcauaucuuc     1680

aaaaaguaca agaccgugag ccacuccaaa uuccucgaaa uuaugcucaa uucuaaccac     1740

cgcgagaauu uuaugaacca uggcgaaaag cugagcaucu ucgggacaca ggaugacaaa     1800

aaguuugcuu ccaaacugag cucuuaucag gauaugacca agauuuucgg agacaucgag     1860

ggcaagcggg cccagauuga ggaaaucauc caauggauua cgaucuuuga ggauaaaaag     1920

auucucgucc agaaacugaa ggaauguuac ccugagcuga cauccaaaca gaucaaucaa     1980

cuuaagaagc ucaacuauag cggcuggggg agacugucug aaaaacugcu cacccacgcc     2040

uaccagggac auuccauuau cgagcugcuu cgccacagcg acgagaauuu cauggaaauc     2100

cugaccaacg auguguaugg cuuucagaau uucauuaagg aggaaaacca gguucaaucc     2160

aacaaaaucc agcaucagga cauugcuaau cucacaaccu cucccgcccu gaagaaaggg     2220

aucuggagca caauuaagcu ggugcgggag cuuacgucca ucuuuggaga accugagaaa     2280

aucauuaugg aguucgcgac cgaagaucag caaaagggca aggagcagaa aucuagaaag     2340

cagcucuggg acgacaacau caaaaagaau aaacuuaaga gcgucgauga auacaaguac     2400

auuaucgacg uggcuaacaa acugaauaac gagcagcucc aacaggagaa gcuguggcuu     2460

uaucuguccc agaaugggaa augcauguac agcggacaau cuauugaucu cgacgcccug     2520

cuguccccca acgccacaaa gcacuaugaa gucgaucaca ucuuuccccg cagcuucauc     2580

aaagacgauu cuauugacaa uaaggugcuu guuaucaaga aaaugaacca gaccaagggc     2640

gaucaggugc cucugcaguu uauucaacag ccguacgagc ggaucgcuua uuggaaaucc     2700

cucaauaagg ccggccugau uagcgacucu aaacuccaua agcugaugaa gcccgaguuc     2760

acagcuaugg auaaagaagg guucauccag agacagcuug ucgaaacccg gcaaauuagc     2820

gugcacguuc gcgacuuucu gaaggaggag uacccuaaca cgaaagugau ccccaugaag     2880

gccaaaaugg ucuccgaguu cagaaagaag uuugauaucc cuaaaauucg gcagaugaau     2940

gacgcgcauc acgccaucga cgcuuaucuc aacggagugg uguaccacgg cgcccagcug     3000

gcuuacccca auguugaucu guucgacuuu aacuucaagu gggaaaaagu cagagaaaag     3060

uggaaagccc uuggggaguu uaacaccaag cagaaaagcc gcgaacuguu cuuuuucaag     3120

aagcucgaga aaauggaggu gucucaagga gaacggcuga uuuccaagau caaacucgau     3180

augaaucauu uuaagauuaa cuauagcaga aaacuggcga auaucccgca gcaguucuac     3240

aaccagacag ccgugucccc uaagaccgcu gagcuuaagu augaaucuaa uaaaagcaac     3300

gaggucgugu acaagggccu gacacccuau caaaccuacg uuguggccau caaauccguc     3360

aauaagaaag ggaaggaaaa gauggaguau cagaugauug accacuacgu guucgauuuu     3420

uacaaauucc agaacggcaa ugagaaggaa cucgcucugu aucuggccca acgcgagaac     3480

aaagacgaag uucuugaugc ccagaucgug uacucucuga auaagggaga ccuccuguau     3540

auuaacaauc auccuuguua cuuugucagc agaaaagagg ugaucaacgc uaagcaguuc     3600

gagcucacgg uugaacagca acugucccuu uauaauguga ugaacaauaa ggagacaaac     3660

gucgaaaaac ugcugauuga guacgauuuu aucgcggaaa aggugaucaa cgaguaucac     3720

cauuaccuca auucuaaacu gaaggagaaa cgggugcgca ccuucuuuag cgaauccaac     3780

cagacacacg aggacuucau uaaggcccuu gaugaacugu uuaaagucgu uaccgcuagc     3840

gccaccagau cugacaagau cggcucccgg aagaauagca ugacacaccg cgccuuccuc     3900

gggaaaggaa aggacgugaa aauugcuuac acgucuaucu ccggccugaa gaccacaaaa     3960

cccaagagcc ucuuuaagcu ggccgagucu agaaacgaac uuuga                     4005


<210>  4116
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4116
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacggauc aguaugaccu gguuaaaaga aagaugaaag uggcuggcaa cuccgauaag      120

aaacaaauca agaagaauuu cuggggagug cggcuuuuug acgagggaga aaccgccgcg      180

gaucgcagaa ugaaccggac agcucgcaga cggauugagc gcagacggaa ucgcaucucu      240

uaccugcagg aaauuuucgc ccucgagaug gccaacaucg acgcuaacuu uuucugcaga      300

cugaaugaua gcuuuuaugu cgacuccgaa aaacgcaauu cucggcaccc uuucuuugcc      360

accaucgagg aggaaguggc uuaccauaag aacuauagaa caauuuacca ccuucgggag      420

gaacugguua auagcuccga gaaagccgau cuccgccugg uguaucuggc gcucgcccau      480

aucauuaagu acagaggcaa cuuccugauc gagggggcuc uugacaccaa aaauacaucu      540

gucgauggag uguacaagca guuuauucag accuauaacc aaguuuucau cagcaauauu      600

gaagagggca cgcuggccaa gauggaagag aacaccacag uggcugacau ccucgccggc      660

aaauuuaccc ggaaggaaaa acuggagaga auccuucagc uguacccggg ggagaagucc      720

acaggaaugu ucgcgcaguu uauuagccuc aucgucggcu cuaaagggaa cuuccagaag      780

guguucgauc ugguggaaaa gaccgacauu gagugugcca aggacuccua ugaagaggau      840

cuggaagcuc uccuggccau cauuggagac gaguacgcug agcuuuuugu cgccgccaag      900

aauacguaua acgcuguugu gcugagcucu aucaucacag ugaccgauac agaaaccaau      960

gcgaaacucu ccgccagcau gauugagcgc uucgacgcuc acgaaaagga ucugcgggag     1020

cuuaaagccu uuaucaagcu gcaucucccc aaacaauacg aagagauuuu cucuaacguc     1080

gccaucgacg gcuaugcugg guacauugau ggaaagacca aacaggugga cuuuuacaaa     1140

uaucugaaga cacugcucga gaauaucgaa ggcgccgauu acuucaucgc gaaaauugag     1200

gaagagaacu uucugagaaa gcagcgcacg uucgacaaug gcgcuauccc ucaccagcuu     1260

caccuggagg aacucgaggc cauucugcau caacaggcca aauauuaccc cuuuuucaag     1320

gaggcuuaug auaagaucaa aucccuuguu accuuucgga uuccuuacuu cguggggccc     1380

cuggccaacg gacagagcga cuuugcuugg cucacaagaa aggccgacgg cgaaauccgc     1440

ccuuggaaua uugaagagaa agucgauuuc gggaaguccg cgguggacuu caucgagaaa     1500

augaccaaca aggauacaua ucugccgaag gaaaauguuc ugcccaaaca cucucucuac     1560

uaccaaaagu auauggugua caacgagcug accaaagucc gguauaucga cgaucaggga     1620

aagacgaauu acuuuagcgg ccaggaaaaa cagcaaauuu ucaacgacua uuuuaagaaa     1680

aagagaaagg uguccaaaaa ggaucuugag caguuccugc gcaauaugag ccauaucgaa     1740

ucuccuacaa uugaggggcu cgaggacucc uuuaacagcu cuuacgccac cuaucacgau     1800

cugcugaaag ucggaaucaa gcaggaagug cuugagaacc cccucaauac cgaaaugcug     1860

gaggacauug uuaaaauccu gacaguguuc gaagauaagc ggaugaucaa ggagcagcuc     1920

caacaguuua gcgacguccu ggauggcgcu gugcuuaaaa agcuggagag acgccacuac     1980

accggcuggg ggcggcucuc cgccaaacug cugguuggaa uuagagacaa gcagucucau     2040

cuuacaaucc ucgacuaccu gaugaacgau gacggccuga aucggaaccu caugcagcug     2100

auuaaugaua gcaaccuuuc cuucaaaagc aucauugaaa aggagcaagu gucuacgacc     2160

gacaaggauc ugcaguccau cgucgcugac cucgccggga gcccugcgau caaaaagggg     2220

auucugcagu cucugaaaau cguggaugaa cuugugucca uuaugggcua ucccccgcag     2280

acaaucguug ucgagauggc ccgcgagaau caaaccacag ugaaggggaa aaacaauagc     2340

agaccucggu acaagucccu cgaaaaggcu auuaaagagu uuggcucuca gauccugaag     2400

gaacacccca ccgacaacca ggagcugaga aauaaccgcc ucuaucugua cuaucuucaa     2460

aauggaaaag auauguacac gggccaggaa cuggacaucc auaaccucag caauuaugau     2520

auugaccaca ucgugccuca guccuucauu accgauaacu cuaucgacaa ccugguccug     2580

acaagcuccg ccgggaaucg ggagaaggga gacgaugugc cuccccuuga gauuguuaga     2640

aaacgcaagg uguuuuggga aaaacucuuc cagggcaacc ugaugagcaa gagaaaguuu     2700

gacuaccuga ccaaagcuga gcgcggggga cucacagaag ccgauaaggc caccuucauc     2760

caucggcaac uggucgagac gagacagauu acaaagaaug uggcuaacau ccuucaccag     2820

cgguucaaua acgaaaccga caaucacggc aacaauaugg agcagguucg caucgugaug     2880

cugaagucug cgcucgucuc ccaauuuaga aaacaguucc agcuguacaa ggugcgggag     2940

guuaacgauu aucaucacgc ccaugacgcu uaccugaaug ggguggucgc caacacacuu     3000

cugaaggugu auccccagcu cgaaccggag uuuguguacg gcgaauauca ccaauucgau     3060

ugguuuaaag ccaauaaggc uaccgccaaa aagcaguucu acaccaacau uaugcuguuu     3120

uucgcgcaga aagagcgcau cauugacgaa aauggagaga uccucuggga uaagaaguau     3180

cuggagacaa uuaaaaaggu ccuugacuac agacaaauga acaucguuaa aaagacggaa     3240

auccagaaag gcgaguuuag caaggcuacc auuaagccca aagggaacuc uuccaagcug     3300

aucccucgga aagaaaauug ggaucccaug aaguacggag gccucgacag cccuaacaug     3360

gccuaugccg ugauuaucga gcacgcuaaa gggaagaaga aaguggucuu cgagaagaaa     3420

auuauccgca ucacaauuau ggaaagaaag gccuuugaga aagacgaaaa gucuuuccug     3480

gagaaacagg gauaccggca gcccaaggug cugaccaaac uuccgaaaua uacacuguac     3540

gaaugcgaga auggcagacg ccggaugcuc gcgagcgcua acgaggccca aaaggggaau     3600

cagcagguuc ugaaaggcca gcucaucacc cugcuucauc acgccaagaa cugugaggcu     3660

uccgauggaa aaagccugga cuauauugaa ucuaauagag aaauguucgg cgagcuccug     3720

gcccaugugu ccgaauuugc uaagcgcuac acgcuggccg augcgaaccu uagcaagauc     3780

aaucaacugu ucgagcagaa caaagacaau gauauuaagg ucaucgccca guccuuugug     3840

aaccucaugg cuuucaaugc caugggggcu ccugccucuu uuaaauucuu ugaggccaca     3900

auugaacgga agagauauac caaccugaaa gagcuccuga gcgcuaccau caucuaccag     3960

uccauuacag gacuuuacga agcgcgcaag cggcuggacg gcuga                     4005


<210>  4117
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4117
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacgaacc aguaugaucu gguuaaaaga aagaugaaag uggcuggcaa uuccgacaag      120

aaacaaauca agaagaacuu cuggggagug cggcuuuuug augacggaca gaccgccguc      180

gaucgcagaa ugaaucggac agcgcgcaga cggauugagc gcagacggaa ccgcaucucu      240

uaccugcagg aaauuuucgc uguggagaug gccaauaucg acgccaacuu uuucugcaga      300

cucaaugaua gcuuuuaugu ugacuccgaa aaacggaacu cucgccaccc uuucuuugcu      360

accaucgagg aagagguggc cuaccaugau aauuauagaa caauuuacca ucugcgggag      420

gaacuuguca acagcuccga gaaggcugac cugcgccucg uguaucuggc ccuggcgcac      480

aucauuaaau acagaggcaa uuuccucauc gaaggggccc uggauaccaa gaacacaucu      540

guugacgagg uguacaaaca guuuauucaa accuauaauc aggucuucau gagcaacauc      600

gaggaaggag cucuugccaa gguggaggaa aaccuggagg uggcuuccau ucucgccggc      660

aaguuuacgc ggagagaaaa auucgagcgc auccugcggc uuuacccggg cgaaaagagc      720

accgggaugu uugcgcaguu caucucucug auugucggaa auaaaggcaa cuuccagaag      780

guuuuuaauc ucguggaaaa gacagauauc gagugugcca aggacuccua ugaagaggac      840

cuggaagcuc ugcucgccau uaucggggau gaguacgcug agcuguucgu ggccgccaaa      900

aacaccuaua augcugucgu gcuuagcucu auuaucacag uuaccgcgac ggaaacaaac      960

gccaagcugu ccgcuagcau gaucgagaga uuugacgccc acgaaaagga ucucggagag     1020

cugaaagccu ucauuaaacu ucaucugccc aaacaauacc aggaaaucuu uaauaacgcu     1080

gccauugacg gcuaugcggg guacaucgau ggaaagacca aacaggugga cuucuacaag     1140

uaucucaaga caacccugga gaaugucgag ggcgcugauu acuuuauuac caaaaucgaa     1200

gaggaaaacu uccugcgcaa gcagcggaca uuugacaaug gcgugauccc ucaccaacuc     1260

caccuggagg agcuugaagc cauucugcau cagcaggcca aauauuaccc cuuccucaga     1320

gaggauuaug aaaagaucaa aucucugguu acguuucgca uuccuuacuu cguggggccc     1380

cuugcuaagg gacaauccga guuugccugg cugacccgga aggcugacgg cgaaaucaga     1440

ccuuggaaca uugaggagaa agucgauuuc ggaaagagcg ccguggacuu caucgagaaa     1500

augacaaaua aggacaccua ucucccgaaa gagaacgucc ugcccaagca cucccugugc     1560

uaccagaagu acauggugua uaaugaacuc acaaaaguuc gcuacauuga ugaccagggg     1620

aagaccaacu auuuuucugg ccaggagaaa caacagaucu ucaacgaucu guuuaagcag     1680

aaacggaagg ugaaaaagaa ggaccuugaa cuguuccuca gaaauaucaa ccauauugag     1740

agcccuacga ucgaggggcu ggaagauucc uuuaaugcga gcuacgccac auaucacgac     1800

cugcuuaaag ucggaaugaa gcaggagucu cucgauagcc cccugaacgc ugaaaugcug     1860

gaggacauug ugaaaauccu caccguuuuc gaagauaagc gcaugauuaa agagcaacug     1920

cagcaguuuu ccgacgugcu ugauggcguc gugcugaaga agcucgagcg gagacacuac     1980

accggcuggg ggcggcuguc ugccaaacug cuugugggaa uccgcgacaa gcagagccau     2040

cucacaaucc uggauuaucu gaugaaugac gacggccuca acagaaaucu gaugcaacuu     2100

auuaacgauu ccaaucuguc uuucaaaagc aucauugaaa aggagcaggu uuccaccaca     2160

gacaaagauc uccagagcau cgucgcugac cuggccgggu cuccugcgau uaagaaggga     2220

auccugcagu cccuuaaaau cguggaugaa cucgugagca uuaugggcua ucccccgcaa     2280

acgaucgucg uggagauggc ccgggagaac cagaccacag uuaaggggaa aaauaacucu     2340

agaccucgcu acaagucccu ggaaaaagcu auuaaggagu uuggcagcca gauccugaag     2400

gaacacccca ccgacaauca agagcucaaa aacaaucggc uguaccuuua cuaucugcag     2460

aacggaaagg auauuuacac aggccaggaa cucgacaucc auaaccuguc caauuaugau     2520

aucgaccaca uugugccuca gucuuucauc accgauaaca gcauugacaa ucugguccuu     2580

acguccucug ccgggaacag agagaaagga ggcgaugugc cuccccucga gaucguucgc     2640

aagagaaaag uguuuuggga aaagcuguac caagggaauc ugaugagcaa acggaaguuc     2700

gacuaucuca ccaaggcuga gcgcggaggc cugacagacg ccgauaaagc cagauuuauu     2760

caucggcagc uugucgaaac ccgccagauc acaaagaacg uggcuaauau ucugcaccag     2820

agauucaaca augagaccga caacuacggg aauacgaugg aacaaguucg gaucgugaca     2880

cucaaauccg cgcuggucag ccaguuccgc aagcaguuuc agcuguacaa agugagagag     2940

gugaacgauu aucaccaugc ccacgacgcu uaccuuaaug gcgucguugc caacacccug     3000

cucaaggugu auccccaacu ggagccggag uucguguacg gagaauauca ucaguuugau     3060

ugguucaagg ccaauaaagc uacagccaag aaacaguuuu acaccaacau caugcuguuc     3120

uuugcgcaaa aggaacggau uaucgacgag aacggcgaaa uucucuggga uaaaaaguau     3180

cuugagacca ucaagaaagu ccuggacuac cgccagauga auauugugaa gaaaacagag     3240

auccagaagg gggaguucuc uaaagcuacg aucaagccca agggaaacuc cagcaaacuc     3300

auuccuagaa aggaaaauug ggaucccaug aaauacggcg ggcuggacuc uccuaacaug     3360

gccuaugccg uuaucauuga acacgcuaag gggaaaaaga agaucgugau ugagaaaaag     3420

cugauccaga ucaauauuau ggagcggaaa auguuugaaa aggaugagga agccuuccuu     3480

gaggaaaaag gcuacagaca ccccaagguc cugaccaaac ucccgaagua uacacuguac     3540

gagugugaga aggggcgccg gagaaugcuc gcguccgcua acgaagccca aaaaggcaau     3600

cagcuggugc uuagcaacca ucugguuucu cuccuguauc acgccaagaa uugcgaggcu     3660

agcgacggaa aaucccugaa guacaucgaa gcccaucgcg aggcuuuuag cgaacuucug     3720

gcccaggugu cugaguucgc gacccgguau acacucgccg acgcuaaccu guccaaaauu     3780

aauaaccucu ucgagcagaa uaaggaaggc gauaucaagg ccauugcuca gagcuuuguc     3840

gaccugaugg uguucaacgc caugggggcc ccugcuuccu uuaaauucuu ugaggcgacc     3900

aucgauagaa agcgcuacac gaaucuuaaa gaacugcugu cuagcacaau uaucuaccaa     3960

uccaucaccg gacucuauga gucucggaag agacuggacg auuga                     4005


<210>  4118
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4118
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacggauc aguaugaccu gguuaaaaga aagaugaaag uggcuggcaa cuccgauaag      120

aaacaaauca agaagaauuu cuggggagug cggcuuuuug acgagggaga aaccgccgcg      180

gaucgcagaa ugaaccggac agcucgcaga cggauugagc gcagacggaa ucgcaucucu      240

uaccugcagg aaauuuucgc ccucgagaug gccaacaucg acgcuaacuu uuucugcaga      300

cugaaugaua gcuuuuaugu cgacuccgaa aaacgcaauu cucggcaccc uuucuuugcc      360

accaucgagg aggaaguggc uuaccauaag aacuauagaa caauuuacca ccuucgggag      420

gaacugguua auagcuccga gaaagccgau cuccgccugg uguaucuggc gcucgcccau      480

aucauuaagu acagaggcaa cuuccugauc gagggggcuc uugacaccaa aaauacaucu      540

gucgauggag uguacaagca guuuauucag accuauaacc aaguuuucau cagcaauauu      600

gaagagggca cgcuggccaa gauggaagag aacaccacag uggcuaacau ccucgccggc      660

aaauuuaccc ggaaggaaaa acuggagaga auccuucagc uguacccggg ggagaagucc      720

acaggaaugu ucgcgcaguu uauuagccuc aucgucggcu cuaaagggaa uuuccagaag      780

guguucgacc ugguggagaa aaccgauauu gagugugcca aggacuccua ugaagaggac      840

cuggaagcuc uccuggccau cauuggagau gaguacgcug agcuuuuugu cgccgccaag      900

aacacguaua augcuguugu gcugagcucu aucaucacag ugaccgacac agaaaccaac      960

gcgaaacucu ccgccagcau gauugagcgc uucgaugcuc acgaaaagga ccugucugag     1020

cuuaaagccu uuaucaagcu gcaucucccc aaacaauacg aagagauuuu cuccaauguc     1080

gccaucgaug gcuaugcugg guacauugac ggaaagacca aacaggugga uuuuuacaaa     1140

uaucugaaga cacugcucga gaacaucgaa ggcgccgacu acuucaucgc gaaaauugag     1200

gaagagaauu uucugcggaa gcagagaacg uucgauaacg gcgcuauccc ucaccagcuu     1260

caccuggagg aacucgaggc cauucugcau caacaggcca aauauuaccc cuuucuuaag     1320

gaggcuuaug acaagaucaa aagccugguu accuuccgca uuccuuacuu uguggggccc     1380

cucgccaaug gacaguccga uuucgcuugg cugacacgga aggccgacgg cgaaaucaga     1440

ccuuggaaca uugaagagaa agucgacuuu gggaagucug cgguggauuu caucgagaaa     1500

augaccaaua aggacacaua ucugccgaag gaaaacguuc uccccaaaca cagccuguac     1560

uaccaaaagu auauggugua caaugagcuu accaaagucc gcuauaucga ugaccaggga     1620

aagacgaacu acuucuccgg ccaggaaaaa cagcaaauuu uuaaugauua uuucaagcag     1680

aaacggaagg ugagcaagaa agaccuggag caguuucuca gaaacauguc ucauaucgaa     1740

uccccuacaa uugaggggcu ggaggauagc uucaacucua gcuacgccac cuaucacgac     1800

cugcuuaagg ucggaaucaa acaggaagug cucgagaauc cccugaacau ggaaaugcug     1860

gaggauauug uuaagauccu caccguguuu gaagacaaac gcaugaucaa ggagcaacug     1920

cagcaguucu ccggcguccu ugauggcgcu gugcugaaga aacucgagcg gagacacuac     1980

acaggguggg gacgccuguc ugccaagcug cuuguuggca uucgggacaa acagagccau     2040

cucaccaucc uggauuaccu gaugaaugac gacgggcuca acagaaaucu gaugcaacuu     2100

auuaacgauu ccaaucugag cuuuaagucu aucauugaaa aagaacaggu guccacaacg     2160

gacaaggauc uccagagcau cgucgcugac cuggccggau cuccugcgau caagaaaggc     2220

auucugcagu cccuuaagau cguggaugaa cucgugagca uuauggggua ucccccgcaa     2280

accaucguug ucgagauggc ccgggagaac cagacaaccg ugaaaggcaa gaauaacucc     2340

cgcccuagau acaaaucucu ggaaaaggcu auuaaggagu ucggaagcca gauccugaaa     2400

gaacacccca cagacaauca agagcuccgg aacaauagac uguaucuuua cuaucugcag     2460

aacggcaagg auauguacac cgggcaggaa cucgacaucc auaaucuguc caacuaugau     2520

auugaccaca ucgugccuca gucuuuuauu acggauaaca gcaucgacaa ucugguccuu     2580

accuccagcg ccggaaaccg cgagaaaggc gaugacgugc cuccccucga gauuguucgg     2640

aagagaaaag uguucuggga aaagcuguuu caagggaauc ugaugucuaa acgcaaguuc     2700

gacuaccuca caaaggcuga gagaggaggc cugaccgaag ccgauaaagc cacauuuauc     2760

caucggcagc uugucgagac ccgccagauu acgaagaacg uggcuaauau ccugcaccag     2820

agauucaaca augaaacaga caaccacggg aauaacaugg agcaaguucg gaucgugaug     2880

cucaaauccg cgcuggucag ccaguuccgc aagcaguuuc agcuguacaa agugagagag     2940

guuaaugauu aucaucacgc ccaugacgcu uaccuuaacg gcguggucgc caauacccug     3000

cucaaggugu auccccaacu ggaaccggag uucguguacg gagaauauca ccaguuugau     3060

ugguucaagg ccaacaaagc uacagccaag aaacaguuuu acaccaauau uaugcuguuc     3120

uuugcgcaaa aggagcggau cauugacgaa aacggcgaga uccucuggga uaaaaaguau     3180

cuugagacca uuaagaaagu ccuggacuac cgccagauga acaucguuaa gaaaacagaa     3240

auccagaagg gggaguucuc uaaagcuacg auuaagccca agggaaauuc cagcaaacuc     3300

aucccuagaa aggaaaacug ggaucccaug aaauacggcg ggcuggacuc uccuaauaug     3360

gccuaugccg ugauuaucga gcacgcuaag gggaaaaaga agauuguguu ugagaaaaag     3420

aucauccgga uuaccaucau ggaacgcaaa gccuucgaga aggaugaaaa gagcuuucug     3480

gagaagcagg gcuacagaca acccaaaguc cuuacaaagc ugccgaagua uacccucuac     3540

gaaugcgaga acgggcggag acgcaugcug gcguccgcua augaggccca gaaaggcaac     3600

caacaggugc ucaagggaca gcugauuaca cuucugcauc acgccaaaaa uugugaagcu     3660

agcgacggca agucucucga cuauaucgag uccaaccggg aaauguucgg ggagcugcug     3720

gcccauguua gcgaguucgc uaaaagauac acccuugccg augcgaaucu guccaagauu     3780

aaccagcucu uugaacagaa uaaggacaac gauaucaaag ugauugccca gucuuucguc     3840

aaucugaugg cuuuuaacgc caugggagcu ccugccagcu ucaaguuuuu cgaggccacg     3900

aucgaacgca aacgguauac aaaucucaag gagcugcuua gagcuaccau cauuuaccaa     3960

uccaucaccg gccuguacga agcgcgcaaa cggcuggacg gguga                     4005


<210>  4119
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4119
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacggauc aguaugaccu gguuaaaaga aagaugaaag uggcuggcaa cuccgauaag      120

aaacaaauca agaagaauuu cuggggagug cggcuuuuug acgagggaga aaccgccgcg      180

gaucgcagaa ugaaccggac agcucgcaga cggauugagc gcagacggaa ucgcaucucu      240

uaccugcagg aaauuuucgc ccucgagaug gccaacaucg acgcuaacuu uuucugcaga      300

cugaaugaua gcuuuuaugu cgacuccgaa aaacgcaauu cucggcaccc uuucuuugcc      360

accaucgagg aggaaguggc uuaccauaag aacuauagaa caauuuacca ccuucgggag      420

gaacugguua auagcuccga gaaagccgau cuccgccugg uguaucuggc gcucgcccau      480

aucauuaagu acagaggcaa cuuccugauc gagggggcuc uugacaccaa aaauacaucu      540

gucgauggag uguacaagca guuuauucag accuauaacc aaguuuucau cagcaauauu      600

gaagagggca cgcuggccaa gauggaagag aacaccacag uggcugacau ccucgccggc      660

aaauuuaccc ggaaggaaaa acuggagaga auccuucagc uguacccggg ggagaagucc      720

acaggaaugu ucgcgcaguu uauuagccuc aucgucggcu cuaaagggaa cuuccagaag      780

guguucgauc ugguggagaa aaccgacauu gagugugcca aggacuccua ugaagaggau      840

cuggaagcuc uccuggccau cauuggagac gaguacgcug agcuuuuugu cgccgccaag      900

aauacguaua acgcuguugu gcugagcucu aucaucacag ugaccgauac agaaaccaau      960

gcgaaacucu ccgccagcau gauugagcgc uucgacgcuc acgaaaagga ucugucugag     1020

cuuaaagccu uuaucaagcu gcaucucccc aaacaauacg aagagauuuu cuccaacguc     1080

gccaucgacg gcuaugcugg guacauugau ggaaagacca aacaggugga cuuuuacaaa     1140

uaucugaaga cacugcucga gaauaucgaa ggcgccgauu acuucaucgc gaaaauugag     1200

gaagagaacu uucugcggaa gcagagaacg uucgacaaug gcgcuauccc ucaccagcuu     1260

caccuggagg aacucgaggc cauucugcau caacaggcca aauauuaccc cuuucuuaag     1320

gaggcuuaug auaagaucaa aagccugguu accuuccgca uuccuuacuu uguggggccc     1380

cucgccaacg gacaguccga cuucgcuugg cugacacgga aggccgacgg cgaaaucaga     1440

ccuuggaaua uugaagagaa agucgauuuu gggaagucug cgguggacuu caucgagaaa     1500

augaccaaca aggauacaua ucugccgaag gaaaauguuc uccccaaaca cagccuguac     1560

uaccaaaagu auauggugua caacgagcuu accaaagucc gcuauaucga cgaucaggga     1620

aagacgaauu acuucuccgg ccaggaaaaa cagcaaauuu uuaacgacua uuucaagcag     1680

aaacggaagg ugagcaagaa agaucuggag caguuucuca gaaauauguc ucauaucgaa     1740

uccccuacaa uugaggggcu ggaggacagc uucaacucua gcuacgccac cuaucacgau     1800

cugcuuaagg ucggaaucaa acaggaagug cucgagaacc cccugaauac cgaaaugcug     1860

gaggacauug uuaagauccu cacaguguuu gaagauaaac gcaugaucaa ggagcaacug     1920

cagcaguucu ccgacguccu ugauggcgcu gugcugaaga aacucgagcg gagacacuac     1980

accggcuggg ggcgccuguc ugccaagcug cuuguuggga uucgggacaa acagagccau     2040

cucacaaucc uggacuaccu gaugaacgau gacggccuca auagaaaccu gaugcaacuu     2100

auuaaugauu ccaaccugag cuuuaagucu aucauugaaa aagaacaggu guccacgacc     2160

gacaaggauc uccagagcau cgucgcugac cuggccgggu cuccugcgau caagaaaggc     2220

auucugcagu cccuuaagau cguggaugaa cucgugagca uuaugggaua ucccccgcaa     2280

acaaucguug ucgagauggc ccgggagaau cagaccacag ugaaagggaa gaacaauucc     2340

cgcccuagau acaaaucucu ggaaaaggcu auuaaggagu ucggcagcca gauccugaaa     2400

gaacacccca ccgacaacca agagcuccgg aauaacagac uguaucuuua cuaucugcag     2460

aauggaaagg auauguacac gggccaggaa cucgacaucc auaaccuguc caauuaugau     2520

auugaccaca ucgugccuca gucuuuuauu accgauaaca gcaucgacaa ccugguccuu     2580

acauccagcg ccgggaaucg cgagaaagga gacgaugugc cuccccucga gauuguucgg     2640

aagagaaaag uguucuggga aaagcuguuu caaggcaacc ugaugucuaa acgcaaguuc     2700

gacuaccuca ccaaggcuga gagaggggga cugacagaag ccgauaaagc caccuuuauc     2760

caucggcagc uugucgagac gcgccagauu acaaagaaug uggcuaacau ccugcaccag     2820

agauucaaua acgaaaccga caaucacggc aacaauaugg agcaaguucg gaucgugaug     2880

cucaaauccg cgcuggucag ccaguuccgc aagcaguuuc agcuguacaa agugagagag     2940

guuaacgauu aucaucacgc ccaugacgcu uaccuuaaug ggguggucgc caacacacug     3000

cucaaggugu auccccaacu ggaaccggag uucguguacg gcgaauauca ccaguuugau     3060

ugguucaagg ccaauaaagc uaccgccaag aaacaguuuu acaccaacau uaugcuguuc     3120

uuugcgcaaa aggagcggau cauugacgaa aauggagaga uccucuggga uaaaaaguau     3180

cuugagacaa uuaagaaagu ccuggacuac cgccagauga acaucguuaa gaaaacggaa     3240

auccagaagg gcgaguucuc uaaagcuacc auuaagccca aggggaacuc cagcaaacuc     3300

aucccuagaa aggaaaauug ggaucccaug aaauacggag gccuggacuc uccuaacaug     3360

gccuaugccg ugauuaucga gcacgcuaag gggaaaaaga agguggucuu ugagaaaaag     3420

auuauccgga ucacaauuau ggaacgcaaa gccuucgaga aggacgaaaa gagcuuucug     3480

gagaagcagg gauacagaca acccaaagug cuuaccaagc ugccgaagua uacacucuac     3540

gaaugcgaga auggccggag acgcaugcug gcguccgcua acgaggccca gaaagggaau     3600

cagcagguuc ucaagggcca acugaucacc cuucugcauc acgccaaaaa cugugaagcu     3660

agcgauggaa agucucucga cuauauugag uccaaucggg aaauguucgg cgagcugcug     3720

gcccauguga gcgaguucgc uaaaagauac acgcuugccg augcgaaccu guccaagauc     3780

aaucagcucu uugaacagaa caaggacaau gauauuaaag ucaucgccca gucuuucgug     3840

aaccugaugg cuuuuaaugc caugggggcu ccugccagcu ucaaguuuuu cgaggccaca     3900

auugaacgca aacgguauac caaccucaag gagcugcuuu ccgcuaccau caucuaccaa     3960

ucuauuacag gacuguacga agcgagaaaa cgccuggacg gcuga                     4005


<210>  4120
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4120
augaagaacc ccuacaccau cggccuggac auugggacaa auagcguggg augggccguc       60

cucacgaacc aguaugaucu gguuaaaaga aagaugaaag uggcuggcaa uuccgacaag      120

aaacaaauca agaagaacuu cuggggagug cggcuuuuug augacggaca gaccgccguc      180

gaucgcagaa ugaaucggac agcgcgcaga cggauugagc gcagacggaa ccgcaucucu      240

uaccugcagg aaauuuucgc uguggagaug gccaauaucg acgccaacuu uuucugcaga      300

cucaaugaua gcuuuuaugu ugacuccgaa aaacggaacu cucgccaccc uuucuuugcu      360

accaucgagg aagagguggc cuaccaugau aauuauagaa caauuuacca ucugcgggag      420

gaacuuguca acagcuccga gaaggcugac cugcgccucg uguaucuggc ccuggcgcac      480

aucauuaaau acagaggcaa uuuccucauc gaaggggccc uggauaccaa gaacacaucu      540

guugacgagg uguacaaaca guuuauucaa accuauaauc aggucuucau gagcaacauc      600

gaggaaggag cucuugccaa gguggaggaa aaccuggagg uggcuuccau ucucgccggc      660

aaguuuacgc ggagagaaaa auucgagcgc auccugcggc uuuacccggg cgaaaagagc      720

accgggaugu uugcgcaguu caucucucug auugucggaa auaaaggcaa cuuccagaag      780

guuuuuaauc ucguggaaaa gacagauauc gagugugcca aggacuccua ugaagaggac      840

cuggaagcuc ugcucgccau uaucggggau gaguacgcug agcuguucgu ggccgccaaa      900

aacaccuaua augcugucgu gcuuagcucu auuaucacag uuaccgcgac ggaaacaaac      960

gccaagcugu ccgcuagcau gaucgagaga uuugacgccc acgaaaagga ucucggagag     1020

cugaaagccu ucauuaaacu ucaucugccc aaacaauacc aggaaaucuu uaauaacgcu     1080

gccauugacg gcuaugcggg guacaucgau ggaaagacca aacaggugga cuucuacaag     1140

uaucucaaga caacccugga gaaugucgag ggcgcugauu acuuuauuac caaaaucgaa     1200

gaggaaaacu uccugcgcaa gcagcggaca uuugacaaug gcgugauccc ucaccaacuc     1260

caccuggagg agcuugaagc cauucugcau cagcaggcca aauauuaccc cuuccucaga     1320

gaggauuaug aaaagaucaa aucucugguu acguuucgca uuccuuacuu cguggggccc     1380

cuugcuaagg gacaauccga guuugccugg cugacccgga aggcugacgg cgaaaucaga     1440

ccuuggaaca uugaggagaa agucgauuuc ggaaagagcg ccguggacuu caucgagaaa     1500

augacaaaua aggacaccua ucucccgaaa gagaacgucc ugcccaagca cucccugugc     1560

uaccagaagu acauggugua uaaugaacuc acaaaaguuc gcuacauuga ugaccagggg     1620

aagaccaacu auuuuucugg ccaggagaaa caacagaucu ucaacgaucu guuuaagcag     1680

aaacggaagg ugaaaaagaa ggaccuugaa cuguuccuca gaaauaucaa ccauauugag     1740

agcccuacga ucgaggggcu ggaagauucc uuuaaugcga gcuacgccac auaucacgac     1800

cugcuuaaag ucggaaugaa gcaggagucu cucgauagcc cccugaacgc ugaaaugcug     1860

gaggacauug ugaaaauccu caccguuuuc gaagauaagc gcaugauuaa agagcaacug     1920

cagcaguuuu ccgacgugcu ugauggcguc gugcugaaga agcucgagcg gagacacuac     1980

accggcuggg ggcggcuguc ugccaaacug cuugugggaa uccgcgacaa gcagagccau     2040

cucacaaucc uggauuaucu gaugaaugac gacggccuca acagaaaucu gaugcaacuu     2100

auuaacgauu ccaaucuguc uuucaaaagc aucauugaaa aggagcaggu uuccaccaca     2160

gacaaagauc uccagagcau cgucgcugac cuggccgggu cuccugcgau uaagaaggga     2220

auccugcagu cccuuaaaau cguggaugaa cucgugagca uuaugggcua ucccccgcaa     2280

acgaucgucg uggagauggc ccgggagaac cagaccacag uuaaggggaa aaauaacucu     2340

agaccucgcu acaagucccu ggaaaaagcu auuaaggagu uuggcagcca gauccugaag     2400

gaacacccca ccgacaauca agagcucaaa aacaaucggc uguaccuuua cuaucugcag     2460

aacggaaagg auauuuacac aggccaggaa cucgacaucc auaaccuguc caauuaugau     2520

aucgaccaca uugugccuca gucuuucauc accgauaaca gcauugacaa ucugguccuu     2580

acguccucug ccgggaacag agagaaagga ggcgaugugc cuccccucga gaucguucgc     2640

aagagaaaag uguuuuggga aaagcuguac caagggaauc ugaugagcaa acggaaguuc     2700

gacuaucuca ccaaggcuga gcgcggaggc cugacagacg ccgauaaagc cagauuuauu     2760

caucggcagc uugucgaaac ccgccagauc acaaagaacg ugaccaauau ucugcaccag     2820

agauucaaca augagacgga caacuacggg aauacaaugg aacaaguucg gaucgugacc     2880

cucaaauccg cucuggucag ccaguuccgc aagcaguuuc agcuguacaa agugagagag     2940

gugaacgauu aucaccaugc gcacgacgcc uaccuuaaug gcgucguugc uaacacacug     3000

cucaaggugu auccccaacu ggagccggag uucguguacg gagaauauca ucaguuugau     3060

ugguucaagg ccaauaaagc caccgcuaag aaacaguuuu acaccaacau caugcuguuc     3120

uuugcccaaa aggaacggau uaucgacgag aacggcgaaa uucucuggga uaaaaaguau     3180

cuugagacaa ucaagaaagu ccuggacuac cgccagauga auauugugaa gaaaacggag     3240

auccagaagg gggaguucuc uaaagcgacc aucaagccca agggaaacuc cagcaaacuc     3300

auuccuagaa aggaaaauug ggaucccaug aaauacggcg ggcuggacuc uccuaacaug     3360

gcuuaugccg uuaucauuga acacgccaag gggaaaaaga agaucgugau ugagaaaaag     3420

cugauccaga ucaauauuau ggagcggaaa auguuugaaa aggaugagga ggcuuuccuu     3480

gaagaaaaag gcuacagaca ccccaagguc cugacaaaac ucccgaagua uacccuguac     3540

gagugugaga aggggcgccg gagaaugcuc gccuccgcga acgaagcuca aaaaggcaau     3600

cagcuggugc uuagcaacca ucugguuucu cuccuguauc acgccaagaa uugcgaggcc     3660

agcgacggaa aaucccugaa guacaucgaa gcucaucgcg aggccuuuag cgaacuucug     3720

gcucaggugu cugaguucgc cacacgguau acccucgcgg acgccaaccu guccaaaauu     3780

aauaaccucu ucgagcagaa uaaggaaggc gauaucaagg cuauugccca gagcuuuguc     3840

gaccugaugg uguucaacgc uaugggggcc ccugccuccu uuaaauucuu ugaggcuacg     3900

aucgauagaa agcgcuacac aaaucuuaaa gaacugcugu cuagcaccau uaucuaccaa     3960

uccaucaccg gacucuauga gucucggaag agacuggacg auuga                     4005


<210>  4121
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4121
augaagaaag aguacaccau cggccuggac auugggacaa acagcguggg augggccguc       60

cucacggaaa auuaugaucu gguuaagaaa aagaugaaag uguacggcaa caccgagaca      120

aaguaucuua agaaaaaucu guggggagug agacucuucg acgaagggga gaccgcugcc      180

gaucggcgcc ugaagagaac aacccggcgc agauacuccc ggcgcagaaa ccggaucugc      240

cgccuucagg accuguuuac agaagagaug aaucaagucg augcgaacuu cuuucacaga      300

cuccaggagu cuuuccuggu gcccgacgaa aaagaguuug aacggcaugc uauuuucggc      360

aagauggagg aggaaguuag cuauuaccgc gaguuuccua ccaucuauca ccugagaaaa      420

caucucgccg auacguccga acaggccgac cugcggcuug uguaccuggc ucucgcccac      480

auugucaagu accgcgggca uuuccugauc gagggagaac uuaauaccga gaacucuagc      540

guguccgaga cauuuagaac cuucauccag guuuauaauc aaauuuuucg ggaaaacgag      600

gugccgcugg cuguccccga uaauaucgaa gaacucuucu cugaaaaggu gagcagagcc      660

cgcaaagugg aggcgauucu guccgucuac agcgaggaaa agucuacagg cacccuggcc      720

caguuucuca aacugauggu uggcaaccag gggcgguuca agaaaacguu cgaccuugag      780

gaagauggaa ucauucagau cccuaaggag gaauaugagg aggaacugga gacacuccug      840

gcuauuaucg gcgacgaaua cgccgagauc uuuuccgcua ccaaaagcgu guaugaugcc      900

guggcgcuuu cugggauucu guccgucaca gacggagaca ccaaggccaa gcucagcgcu      960

ucuauggugg agagauacga agcccaccaa aaagaucugg uucaguucaa gcaguuuauc     1020

cgcaaagagc ugcccgaaau guaugcuccu auuuuccggg acaauuccgu gagcggcuac     1080

gccggguacg ucgagaacuc caaggugguu acccaggccg aauuuuauaa auacaucaag     1140

aaggcuauug agaaagugcc cggagcggag uauuuccucg aaaagaucga gcaagaaaca     1200

uuucuggaua aacagagaac guucaauaac ggcgucaucc cucaccagau ucaucuugag     1260

gaacuggagg ccaucauuca aaagcaggcu accuacuauc cguuucucgc cgacaacaaa     1320

gaggaaauga agcagcuggu gacauuccgc auccccuacu augucggccc ucuugccgau     1380

ggaaauucuc ccuuugcuug gcuggagcgg auuagcuccg aaccuaucag acccgggaac     1440

cucgccgagg ugguugacau caagaaaagc gcgaccaagu ucauugaacg caugacaaau     1500

uuugauaccu accugccgac ggagaaagug cugccuaagc acucuaugau cuacgagaaa     1560

uauauggucu acaacgaacu cacaaaggug uccuauguug acgagcgggg caugaaucag     1620

agauucagcg gggaagagaa gaaacaaauu guggaggaac uguucaagca gucucgcaaa     1680

gucaccaaga aacuucugga gaaguuucuc agcaacgagu ucggacuggu ggauguggcu     1740

aucaaaggca uugagaccuc cuuuaaugcc ggguacggaa cauaucauga cuuccugaag     1800

aucggcauua cccgggaaca gcuugauaag gaggagaacu cugaaacacu cgaggaaauc     1860

guuaaaaucc ugacggucuu ugaggacaga aagaugauuc gggaacagcu gaaaaaguac     1920

accuaucucu ucgacgagga ggugcugaaa aagcuggaac gcagacacua cacaggcugg     1980

gggcggcuua gcgccaagcu ccugaucgga auuaaagaga agagaaccca caaaacaauc     2040

cuggauuacc uuauugaaga cgauggcggg aagcaaccca ucaaucgcaa ccucaugcag     2100

cugaucaaug acuccgaucu gagcuuuaaa ucugagauug cugaagccca guccgacaug     2160

aacaccgagg aucuccauga ggugguccag aaucuggcug gaagcccugc caucaagaag     2220

ggcauucuuc aaucucugaa aaucguggac gaacucguug auauuauggg gucccugccc     2280

aagaacaucg uggucgagau ggcgcgggaa aaucagacga ccagcagagg ccgcacaaac     2340

uccaacccca gaaugaaagc ccuggaggag gcuaugcgga aucuucgcuc uaaccuccug     2400

aaggaauauc cuaccgacaa ucaggcccug cagaacgaua gacucuaccu guauuaccuu     2460

caaaauggaa aagacaugua uacaggccug gaucucagcc ugcacaaccu guccucuuac     2520

gacaucgacc auauugugcc gcagagcuuc accacggaua auucccuuga caaccggguu     2580

cucgugagcu cuaaggagaa ucgcgggaag aaagaugacg uccccuccaa ggaagugguu     2640

cagaaaaaca ucacacugug ggagacccug aagaauagca accucauuuc ucaaaagaag     2700

uaugauaauc ugacaaaagg acuuagaggc gggcugaccg aagacgaucg ggcucacuuu     2760

aucaagcgcc agcucgugga gaccagacag auuacaaagc augucgcccg gauccuggac     2820

cagcgcuuca acucccaaaa agaugaggaa ggaaagacga ucagagcggu gcgggugguc     2880

acccugaaaa gcucucuuac aagccaguuu agaaagcagu ucgccauuca caaaguucgc     2940

gagaucaaug acuaccacca uggccacgau gcuuaccuga acgggguggu ggccaacucc     3000

cuccugcggg ucuauccuca gcuccaaccc gaauuugugu acggcgacua uccuaaguuc     3060

aaugcuuaca aggccaacaa agccaccgcu aagaaacagc uguauacaaa uauuaugaag     3120

uucuuugcgg aggaugccgu uaucauugac gaaaacggag agauccuuug ggacaaaaag     3180

aauauugcua ccgugaagaa agucaugagc uacccccaga ugaacaucgu gaagaaaccg     3240

gagauccaga cgggcucuuu cuccaaggaa acaauuaaac cuaaggggga uagcgacaag     3300

cugaucucca gaaaaaccaa uuggucuccc aagcucuaug gaggcuuuga uagcccucaa     3360

guugccuacu ccgugauuau caccuacgag aaagggaaga aaaagguccg cgccaaggcu     3420

auugugggaa ucacaaucau ggaacagucu cuguucaaaa aggaccccgu uagccugcuu     3480

gaggaaaaag gcuaugccaa cccugaggug cugauucauc uccccaagua cacccuguau     3540

gagcucgaaa augggcggag acgccugcuu gcguccgcua acgaggccca gaaaggcaau     3600

caacuggucc ucccggccuc ucuggugaca cugcuuuacc acgcuaaaca gguggaugaa     3660

gacagcggaa aauccgagga guauguccgg gaacacagag ccgaguuugc ggaaauccug     3720

aacuacguuc aggcuuucag cgagacgaag auucucgcca auaagaaccu gcagaccauc     3780

cucaaacugu augaagagaa uaaggaggcc gauauuaaag aaaucgcuga gucuuuugug     3840

aaccuuauga aguucuccgc cuacggcgcu ccuauggacu uuaaauucuu ugggaagaca     3900

aucccccgca gccgguacac cucuguggga gaacugcugu ccgccacaau uaucaaccaa     3960

agcauuaccg gccucuauga gacgagacgg aagcuggucg auuga                     4005


<210>  4122
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4122
augagaaagc ccuacaccau cggccuggac auugggacaa acagcguggg augggccguc       60

cucacggauc aguauaaucu gguuaaacgg aagaugaaag uggcuggcuc cgccgagaag      120

aaacaaauca agaagaacuu cuggggagug cgccuuuuug acgaaggaga ggucgcggcu      180

ggcagacgga ugaaucgcac cacaagacgg cgcauugaaa gacggcgcaa cagaaucgcc      240

uaccugcagg agauuuucgc cgcugaaaug gccgaggugg augcuaauuu cuucuaucgg      300

cucgaggacu cuuuuuacau cgaaagcgag aaacgccacu ccagacaucc uuucuuugcc      360

accaucgaag aggagguugc guaucacgaa gaguacaaga caauuuauca ucugcgggaa      420

aaacuuguga acucuagcga uaaggccgac cugcgccucg ucuaccuggc ucuggcccac      480

aucauuaaau acagagggaa uuuccucauc gagggaaugc uggauaccaa gaacacaucc      540

guggacgaag uuuuuaagca guucauucag accuauaauc aaaucuuugc uucugauauu      600

gaggagggca gccuuacgcg gcuggaagag aacaaagaag uggccgagau ccucuccgaa      660

aagcugacca gacgcgagaa acuugacaag auccugaaac ucuacacagg cgaaaagagc      720

accgggaugu ucgcgcgguu uauuaaucug aucauuggau cuaaaggcga uuucaagaag      780

gucuucgacc uggaugaaaa agccgagauc gaaugcgcua aggacacaua ugaggaagac      840

cucgaggccc ugcuugcuaa aauuggggau gaguacgccg aaaucuuugu ggccgcuaag      900

uccaccuaua acgcgguggu ccugagcaau aucauuacgg uuacagacac cgagacaaaa      960

gccaagcucu cugcuuccau gaucgaaaga uucgauaagc augccaaaga ccugaagcgc     1020

cuuaaagccu uuuucaagau gcagcugccg gagaaauuua acgagguguu caaugauauu     1080

gaaaaggacg gauacgcugg cuauaucgau gggaagacca cccaggagaa auuuuacaag     1140

uacaugaaaa agaugcucgc caacauugac ggagcggauu auuucaucga ccagaucgaa     1200

gaggaaaacu uucugcggaa acaaagaaca uucgauaaug gcacgauucc ccaccagcug     1260

caccucgagg agcuggaagc uauccuucau cagcaggcca aguacuaucc uuuucugcgc     1320

aaggacuacg agaaaauucg gagccucgug accuucagaa uccccuauuu uauuggcccu     1380

cuggccaacg ggcaaucuga cuucgcuugg cuuacacgca aggccgaugg agaaauccgg     1440

cccuggaaua uugaggaaaa agucgacuuc ggcaaguccg cuaucgauuu uaucgagaaa     1500

augaccaaca aggacacaua ccugccuaaa gagaaugugc ucccgaagca cagccugugu     1560

uaucagaagu acaugguuua caacgaacug accaaaauua gauauaucga ugaccagggg     1620

aagacgcauc acuucuccgg acaagagaaa cagcagauuu uuaauggccu cuucaagcag     1680

caacgcaaag ugaagaagaa agaucuggaa cgguuucuuu acacaaucaa ccacauugag     1740

ucucccacca ucgaaggggu cgaggacgcc uucaauagcu ccuuugcgac cuaucaugau     1800

cugcagaagg gaggcgugac acaggagauc cucgacaacc cucugaaugc cgauaugcug     1860

gaagagauug uuaaaauccu uaccguguuc gaagacaaga gaaugauuaa agagcagcuc     1920

cagagcuuuu cugauguccu ggacggcaca auccugaaga agcucgagcg gcgccacuac     1980

acgggguggg gaagacuguc cgcuaaacuu cugaccggca uucgggacaa gcauagccac     2040

cucacaaucc uggauuaucu gaugaacgac gaugggcuua auagaaaccu caugcaacug     2100

aucaaugacu cuaaccugag cuucaaaucc auuaucgaaa aggaacaggu gucuaccgcc     2160

gauaaaggga uucagagcau cgucgcugaa cucgccggcu cccccgcgau uaagaagggg     2220

auccugcaaa gccuuaaaau cguggacgag cugguuggca uuaugggaua cccuccccag     2280

acaaucgugg ucgaaauggc ccgcgagaac cagaccacgg gcaaggggaa aaauaacucu     2340

aagccgcggu uuauuucccu cgagaaagcu aucaaggaau uuggaagcca gauucugaaa     2400

gagcauccua ccgauaauca augccugaag aacgacagac uuuaucucua cuaccugcag     2460

aauggcaagg auauguauac agggaaagaa cuggacaucc acaaccucuc uaauuacgau     2520

auugaccaca ucauccccca guccuuuauu accgauaaca gcaucgacaa ucgcgugcug     2580

guuuccucua cagccaacag agagaaggga gacaaugugc cucuucugga agucgugcgg     2640

aaacgcaagg cuuucuggga gaaacucuau caagccaagc ugaugagcaa gagaaaauuc     2700

gauuaccuga ccaaggccga gcggggcggg cuuacggaag cugacaaagc gaacuuuauu     2760

cagcgccagc ucguggagac aagacagauc accaagaaug uugccaacau ucuguaucaa     2820

cgguucaauu guaaacagga ugaaaacggc aacgaggucg aacaggugcg caucgugaca     2880

cugaagucca cccucgucuc ucaguuuaga aagcaauucc agcuguacaa agugcgggag     2940

guuaaugacu aucaucacgc ucaugaugcc uaccuuaacg gaguggucgc caauacccug     3000

cucaaggugu acccccagcu ggagcccgaa uuuguuuaug gcgacuacca ccaguucgau     3060

ugguuuaaag cuaacaaggc cacagcgaaa aagcaauucu auacgaauau caugcgcuuu     3120

uucgcuaagg agaaccagau uaucgacaaa aauggggaaa uucuguggga uaacagauac     3180

cuugacacca ucaagaaagu gcugagcuau cggcagauga auauugucaa gaaaacagag     3240

auccaaaagg gagaguuuuc caacgccacc gugaauccua agggcaacag cucuaaacuc     3300

aucuccagaa aggccgauug gaauccgauu aaauacgggg gauucgacgg cagcaacaug     3360

gcuuauucua ucguuauuga auacgagaag cgcaaaaaga aaacagugau caagaaggaa     3420

cugauucaga ucaauauuau ggagcggguc gccuucgaaa aagaccagaa ggcguuucuc     3480

gaggagaaag gguacuauuc ccccaaggug cugaccaaaa ucccuaagua cacgcuuuau     3540

gaaugcgaga acggcagacg ccggaugcug ggaagcgcua acgaagccca gaagggcaau     3600

caaauggugc uccccaacca ccugaugaca cugcuuuacc augccaaaaa uugugaggcu     3660

aacgaugggg aaucucuggc cuauaucgag augcacagag agauguucgc ugaacuccug     3720

gccuacauua gcgaguuugc gaagcgcuau acccucgcca augaccggcu ggagaaaauc     3780

aacauguucu uugagcagaa uaagaaagga gauauuaagg ucaucgcuaa guccuucgac     3840

aaacuuaagg uuuuuaacgc cuucggcgcu ccuagagauu uugaguucuu ugagaccaca     3900

auuaaacgca agcgguacua caauaucaaa gagcugcuca acgccaccau cauuuaucag     3960

agcaucacag ggcuguacga agccagaaag cggcuggagg acuga                     4005


<210>  4123
<211>  4008
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4123
augaagaacu acaccaucgg ccuggacauu gggacaaaua gcgugggaug ggccgucauc       60

aaagaugacc ucacgcuggu uagaaagaaa auuaagaucu ccggcaacac cgauaaaaag      120

gaggugaaga aaaaucuuug ggggucuuuc cuguuugaac agggagacac agcucaagau      180

acccggguga agcgcauugc cagacggcgc uaugagagac ggcgcuucag aauccgggaa      240

cuccagaaaa ucuuugacaa gagcaugggc gaggucgauu ccaacuucuu ucaccgccug      300

gacgaaucuu uccuugugga ggaggauaaa gaauacagca aguaucccau uuuuagcaau      360

gagaaggaag acaaaaacua cuaugauaag uacccuacaa ucuaucaucu gagaaaagac      420

cucgcggauu cuaaucagaa ggcugaccug cggcugauuu accucgcccu ggcccacaug      480

aucaaauacc gcgggcauuu ccuuauugag ggagaccuga agauggaugg caucagcauu      540

uccgagagcu uucaggaguu caucgacucu uauaacgaag uuugcgcucu cgaagaugag      600

aaucuggaaa ucauuuacaa cgacgagcuu cugacccaaa ucgagaauau uuuuaaacag      660

gauaucucca gaagcaagaa gcucgaccag gccauugcuc uguuccaggg cgugaaacgg      720

caaucucugu uugggaucuu ccucacacug aucgucggaa acaaggccaa uuuccagaaa      780

auuuuuaacc uugaagauga caucaagcug gaucucaaag aggaagacua ugaugagaau      840

cuggaggaac uucuguccaa cauugacgag ggcuaccgcg acguguuccu ccaggcgaag      900

aacguuuaua augccaucga acugagcaag auucugaaaa ccgaugggaa ggagacgaaa      960

gcuccgcucu cugcccagau gguggaacug uacaaccaac acagagagga ccuuaagaaa     1020

uauaaggauu acaucaaggc uuaccugccc gaaaaauaug gagagaccuu uaaggacgcc     1080

accaaaaaug gcuacgcggg guauaucgau ggaaagacau cccaggagga cuucuacaaa     1140

uuugucaagg cccagcucaa gggcgaagag aacggcgaau auuuccugga ggcuauugag     1200

aaugaaaacu uucuucggaa acaaagaucc uucuacaaug gggugauccc uuaucagauu     1260

caucugcagg agcucacagc cgugcuggau cagcaagaaa agcacuacuc cuuucugaaa     1320

gagaaccgcg acaagaucau uucucuccug accuuccgga uccccuacua ugucggaccu     1380

cuugcuaaag gcgaaagcag auuugccugg cuggagcgcu ccaauagcga ggaaaagauu     1440

aaacccugga acuucgauaa gaucguugac aucgauaagu cugccgagcu cuuuauugaa     1500

aaucugacgu cccgggacac auaccugccu gaugagccgg ugcuucccaa aagaagccuc     1560

aucuaucaga aguucaccau uuucaacgaa cugacaaaaa ucucuuacau ugacgagcgc     1620

gggauccugc agaauuuuag cucccgggag aagaucgcua uuuucaacga ccucuuuaaa     1680

aauaagucua aggugaccaa aaaccagcug gucaaguaua ucgaaaacaa agagcaaauu     1740

aucgcgccug aaauuaaggg aaucgaggau agcuucaauu ccaacuacag caccuauauc     1800

gaccuuucua aaauucccga uaugaagaag cugcucgaaa aagacgagga ugagauccug     1860

gaagagauua ucaagauucu gacaaucuuu gaagacagaa aaaugcgcaa gcggcagcuu     1920

augaaauuca aggauaagcu cuccgagaaa gccaucaauc agcugagcaa gaaacacuac     1980

acgggcuggg ggcagcuguc ugagaagcuc auuaacggaa ucagagacga acaauccaau     2040

aaaaccauuc uggauuaccu uaucgaggac aacggcuguc cuaagaauau gaaccgcaau     2100

uuuaugcagc ugauuaacga ugacacacuc agcuucaaag aaaagauccg gaaggcucag     2160

gauauuaauc aggugaacga caucaaagag aucguuaagg accugcccgg cuccccggcc     2220

auuaaaaagg ggaucuauca aucuauuaga aucguggaug aaauuauccg gaaaaugaag     2280

gaccgcccua agaauaucgu cauugagaug gccagagaga accagaccac acaggaaggc     2340

aaaaauaaga gcaaagcucg gcugaagaaa auccaagagg gacuugaaaa ccucgauucc     2400

gugcauguug agaagcaggc ccuggacgaa gagaugcuga agucucccaa auacuaucuc     2460

uacugccugc agaacgggaa ggauauuuau accggaaaag accuugauau cggccagcug     2520

caaacguacg acauugauca caucaucccu cgcagcuuua uuacagacaa uuccuucgau     2580

aaccucgugc ugaccagcuc uaccgucaau agagggaagc uggacaacgu gcccuccccc     2640

gacaucgucc ggcagcagaa aggcuuuugg aagcagcuuc ucagagcggg acugaugagc     2700

caacgcaagu ucaauaaccu gacaaaaggc aagcucaccg auagagaccg gcagcaguuu     2760

auuaaucgcc agcuggugga gacaagacaa aucacgaaac auguugcuaa ccuucugucu     2820

caccaccuca augaaaagaa agaggugggg gaaauuaaca ucguccugcu gaaguccgcc     2880

cuuaccagcc aguuccggaa gaaauucgau uuuuauaagg ugcgcgaggu uaaugacuac     2940

caucacgccc augaugcuua ccugaacgga gugauugccc ucaaacugcu cgaacuguau     3000

ccuuacaugg cuaaggaccu uaucuauggc aaauacucuu aucacagaaa gaucgagggg     3060

gauaaagcca cacaggcgaa guacaagaug agcaauauua ucgagcgguu cucccaagac     3120

cugcucgcca acccggaugg agaaauugcu ugggagaaag acaaggaucu gaauaccauc     3180

cgcaaagucc ugagcucuaa gcagauuaac aucaucaaaa aggccgaaga gggcaagggg     3240

agacuuuuua aagagacaau uaacucccgg cccagcaaga aaaccgaaaa gcgcaucccu     3300

auuaaaaaua accuggaccc caauaucuau ggcggauaca uugaggaaaa gauggcuuac     3360

uauaucgcca ucaacuaccu cgagaauggc aagacgaaaa aggcgauugu ggggaucucc     3420

auuaaagaua agaaagacuu cgaaggacag accacagagu aucugggcaa gaucggguuu     3480

aacaaggccu cuauuaucaa uagcuucaaa aacuacaccc ucuuugagcu ggaaaaugga     3540

uccagacgga ugauuguggg cgcuucuaag gagaacgaca gcaaagggga acuucagaag     3600

ggcaaucaaa uguaucugcc ucagaaccuc cuggaguucg uuuaccaucu gaaacacuau     3660

aaugaagaug agacauccca caaguuuauc gucgagcaua aagccuacuu cgacgaacuu     3720

cugaacuaca ucguggaguu ugcuaauaag uaucucgaac uggagaacuc uauugaaaag     3780

aucaaagauc ucuaccacgg aaagggcccc gacguggagg agaaagaacu ggucgagagc     3840

uucauuaauc uucuggccau caccaagugu gggccggccg cugauauuac guuccuggga     3900

gagaaaaucu ccagaaagcg cuaucggagc acaaacugcc ucuggggcuc ugaagugauc     3960

uuucaguccc cuaccgggcu guacgagaca agacuucgcc uggaauga                  4008


<210>  4124
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4124
auggacgaua acuaccagcu gaugcacgcc aagggcaaau auggguacgg aaccagaauc       60

uauagcgagg gccaaacagc ugccgaccgg cgcggguuca gaacgacccg gcgcagacuc      120

aaucggcgca aguggagacu gcggcuucug caggacuuuu ucgaacccua cauucucccu      180

caggaugagg cguuuuucau ccgccagaaa caauccaacc uggugccgca ggauacaaag      240

aaacaguauc ccgaaucugu ccuuuuugac gauaccagcg acaaggauuu cuacacagag      300

uauccuacca uuuaccaucu gagacaggcu cucaugacag aaaaucggca auuugacauc      360

cgcgagguuu accuggccau ucaccauauc gugaaguaua gaggacacuu ccugaacaau      420

gcccccguga aaaccuuuaa guccggcaac cucgaucugg cuaucaaauu caaugagcuu      480

aacgaagagu uuaagucugu cuacccugac cuggaacucc aguucaaucu ggaucagauu      540

gacgagauca aaaagauucu ucuggacagc acgaaguccc gguuugaucg ccagggggag      600

gccgugaaac ucaucuauac cguuucugaa gugaagacaa uugagaaacu gcaaaacaag      660

aucgucaaac aguuccugag cggcauucuc ggacugaaua ccagauucga cgugcuuaca      720

aacaccgaau ccgaugacag cauguggaag uuuucucugg auuccgagag cucugacgau      780

gcucucgacg aaaucaucgc ccagcuggau gaccccgcga uucauguugu ggagcuuguc      840

aaugagaucc acuccagcau uacgcuggcc ggcaucgugc cugaagggca aggacucucu      900

gaguccaugg uggaaaaaua cgaggcucac aaggcccauc ugcagcugcu caaggcuguc      960

gaaaaacugg uugauaagaa agccgcggac aaacuugagg uggccuauga ccaguacauu     1020

aacggcaaag auagcaagca guucaucaag gaggacuuuu auaaagcugu gcaauccgaa     1080

aucaaggggg ucgccggaga ugagguggcu gaaauuaauc agcugaucga gcucgaccag     1140

uucaugccga aacagcggac aaaggaaaac ggcgccauuc cccaccaacu gcaucagcac     1200

gagcuugaug uuaucauuga gaaccagucu aaauacuacg acuggcuggg ggaaaaggug     1260

ggaaaugagu auaagcucga uaaacugguc agcuuuagag ugccuuacua uguuggcccc     1320

cugguggaca ccgccuccaa ggaaaagaag gaugagacac gcuucgcuug gaugguccgg     1380

aaaaacccug agagcaccga agcgaucacc cccuggaauu uugaggacau cguggauaag     1440

aacgccucug cugaaaauuu cauuaagaga augacaacga aagacaagua ccuccugaac     1500

gaggaugucc uuccgaaaug cucccugcuc acccaggacu uugaagugua uaaugagcug     1560

aacaauaucc gcguuaacgg cgagaaguuc acagccgaac aaaaacagcu gauucacgac     1620

gagcuuuuua ccaagcaugc cagcgugucu aucaaacagc ucaaggauuu ccuggucagc     1680

gaacugaugu acgugcggga caaucucaag guugaggggc uggcugauga aaagaaacuu     1740

auguccucuc ugagcacaca guuugacuau caaaagauua ucccugauca gauuaacgac     1800

gugaaguaca gagaggauau cgagcagauc auugaauggu ccaccaucuu cgaggacgcc     1860

gcgauuuuca aaacgaagcu caaggaaauc gauuggcuga cagacgagca acgcaauaaa     1920

cuggcuaaca agcgguacag aggauggggc agccuuucua aacgccuccu ggccugucug     1980

gucgaugaaa augggcagcg gauuaucgac auccucagag auuccagcga caacuuuaug     2040

augauuguga accgccccga guucaagcag gccaucuuug acgagaaugc uaaccugacc     2100

gaaaccaauu cuaaaaagga uauguucgag augauugaca acuuuuauac auccccucag     2160

aauaagaaag ccauccggca agugcuucug guuguccagg auauucagaa cgcuauggga     2220

ggcguggcgc cccagaagau cuucaucgaa uuugccagag aggacgaggu gaaugggcgg     2280

cgcaccaaca gcagauacuc ccaaauucgg gaaauguauu cuaaagucac agaugacgug     2340

aucaauaaga cgguuagagg agagcucgaa gccaacaaag gcagccugaa uaaggagaag     2400

cuguuccuuu acuuucucca gggcgggauu gaucuguauu ccggaaaaac caucaacauu     2460

gacaauaucu cuagcuacga uguggaccac guccugcccc aguccuucau caaggaugac     2520

agcauuaaca auaaagugcu cguuaacgcu aagggcaacc gcgugaaaaa ugacaacguc     2580

ccuauggaac uguuuggggc caagauguau ccgacaugga aacggauggu ggauaccgga     2640

aucaugucua aggcuaaguu caaaaaucuu acacugaacc ccgacaaugu ugauaaguuu     2700

aaagccgagg gcuucauuaa cagacagcuc guggagaccc gccaaaucau uaagcugguc     2760

gcgcagaucc ugaaugacaa acuuggggau gacacgaaga uuguguccgu gaaggccaac     2820

cucacccaug auaugagagu cuucuuugac uucccuaaaa aucggaacgu uaauaacuac     2880

caccaugcuu uugaugccua ccugagcgcu uucgugggca cauaucugua caagcgcuau     2940

cccaaacucc agccuuacuu uguguaugga gaguucaaga aaauggacaa gcugaagaau     3000

augaccucuu uugauuuccu uagagaccug aaacacacag auaaggucgu ggacaaagac     3060

accggcgaaa ucaucgccga uaagcucgcc guuauuacga acaugaauaa aaucuacgac     3120

uuuaagaagg ugcuggucac acacgaagug caugagaacu ccggggcuau guaugaucaa     3180

acccuguacc ccgcgaaacu ugacaagagc cugggacgga aaggcgccaa gcagcucauu     3240

ccgaaaaagg augacaugcc uacagcucug uacgggggau auaccggcuc uaaggaugcc     3300

uucaugagca ucguucgccu caaaaagaaa gacgaagugu acuauaaggu cauugggauc     3360

cccgugagaa ucgccgauuc cguucagcac ggcaaagagg uggacgucga ggcucugacc     3420

caguuuauug aaacaaaguu cacgaaagag aaccuuaaua agaagaccgg agaaaucaca     3480

cggaaagugg agugguucga acuggugcuc ccuaaggucc uguuuggcca agccguuuuc     3540

gaugggggac agcccuuuau gcugggcagc ucuaccuacc cucauaaccu ucgcgagcug     3600

gugcucacaa aagacuccgu gaagaaaauu gcggggaagg agagcaauaa guccuaucug     3660

gaugcuuacg acgaaaucau ggaggccaug aacaaauauu ucccccucua cgacaccaau     3720

aaguuuagag aaaaagucau ugagggacgg ucuaaguuca ucgaucugcc ggacaaagau     3780

gaguacgaaa acaauaaacu ugugcagguu ggcaagagca cgaucauuga ccgcauguuu     3840

aucgggcugc acgccaacgc ugccagaaca gaucucaaaa auauuggcau caccaccccu     3900

uucggauuua ugcagcaagc guccggcauu aagcugacaa aagacaccgu gguccuguuc     3960

cagucuagca cagggcuuuu ugagcggaag guggcucuga aagaucucug a              4011


<210>  4125
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4125
augaccaagg acuacacaau cggccuggau auugggacga acagcguggg augggccguc       60

cucaccgacg auuaucagcu gaugaaaaga aagauguccg uucacggcaa uacagagaaa      120

aagaaaauca agaagaacuu cuggggagcu cggcuuuuug acgaagggca aaccgccgag      180

uuccgcagaa caaaacggac caaucgcaga cggcuggcgc gcagaaagua ccggcucucu      240

aaacugcagg aucuuuuugc ugaggaacug ugcaagcagg acgauuguuu cuuugugcgc      300

cucgaagaga gcuuccuggu gcccgaggaa aaacaguaua agccugccuc cauuuuuccg      360

acacuggagg aagagaagga guacuaucaa aaauacccca ccaucuauca ucucagacag      420

aagcuggucg acucuacgga aaaagaggau cuucggcugg uguaccucgc ccuggcucac      480

cuucugaagu accgcggcca uuuccucuuu gaaggguauc uggacaccga gaacacaagc      540

auugaagagu ccuucagagu uuuucuggag caguacggaa aacagucuga ucaaccucuc      600

aucgugcacc agcccguccu gaccauccuu acagacaagc ugagcaaaac caagaaggug      660

gaagagauuc ucaaauauua cccuacggaa aagaucaauu ccuucuuugc ccagugccug      720

aaacuuauug uuggcaacca ggcuaauuuc aagcggaucu ucgaucugga ggccgaagug      780

aaacuccaau uuagcaagga gacauaugag gaagaccugg agucucugcu cgaaaagauu      840

ggcgacgagu accuggauau cuuccuucag gcgaaaaagg uccaugacgc cauucugcuc      900

uccgaaauca ucagcucuac cgugaaacac acacaggcua agcuguccag cgggauggug      960

gagcgcuaug agagacacaa agccgaucuu gcuaaguuua agcaauucgu caaagaaaac     1020

guuccccaga aggccaccgu guuuuucaaa gacaccacaa agaauggaua cgcgggcuac     1080

auuaaaggga agacgaccca ggaggaauuu uauaaguucg ugaaaaagga gcugucugga     1140

gucgugggcu ccgagccuuu ucucgaaaag aucgaucagg agacauuccu gcugaagcaa     1200

cggaccuaca caaacggggu uauuccgcau caggugcacc ucaucgaacu gaaagccauu     1260

aucgaccagc agaagcaaca uuaucccuuu cuugaggaag cuggaccuaa aaucauugcc     1320

cuguucaagu uuagaauccc cuacuauguc ggcccucucg cuaaggagca ggaggccagc     1380

uccuucgccu ggauugaacg caaaaccgcu gagaagaucc accccuggaa uuucucugaa     1440

gugguugaua uugagaaaag cgcgaugcgg uuuauccaga gaaugacgaa gcaggacaca     1500

uaccugccga ccgaaaaagu gcugccuaag aacucccuuc ucuaucaaaa guacaugauc     1560

uucaaugagc ugaccaaagu cagcuacaag gaugagcgcg gcgugaaaca guauuuuucu     1620

ggggacgaaa agcagcagau uuucaaacaa cuguuucaga aggagcgggg aaagaucaca     1680

gucaaaaagc uccagaacuu ccuguacacc cacuaucaua uugaaaaugc ccacaucuuu     1740

ggcauugaga aggcuuucaa cgccuccuac agcacauauc augauuuuau gaaacuugcc     1800

aaaacgaacc aaaagucuau gcaggaaugg cuggagcagc ccgagaugga accuaucuuc     1860

gaggacauug ugaagauccu caccaucuuu gaagacagac agaugauuaa acaccaacug     1920

agcaaguacc aggagguuuu cggggagaaa cugcuuaagg aauuugcucg caaacauuau     1980

acaggauggg gccgguucuc cgccaagcuc auccacggga uuagagaucg caaaaccaau     2040

aagacaaucc uggacuaccu gauuaacgau gacgaugugc ccgcgaaucg gaacagaaau     2100

cucaugcagc ugaucaacga cgagcaccuu ucuuucaagg aagagaucgc uaaagccacc     2160

gucuuuagca agcauaaauc ccugguggau guuauucagg accuccccgg aagcccugcc     2220

aucaagaaag gcauuuggca aucucugaag aucguggagg aacugauugc uaucaucggc     2280

uacaagccga aaaauauugu caucgagaug gcccgcgaaa accagaagac gcaccggacc     2340

aaacccagac uuaaggcucu cgagaauggg cugaaacaga uuggauccac acugcucaag     2400

gaacagccua ccgauaacaa ggcccugcaa aaagagcggc uuuaucugua cuaucuccag     2460

aauggccgcg acaucuacac aggggaaccc cuggauauug agaaccugca ucaguaugag     2520

guggaccaca ucaucccucg gagcuucauu guggauaauu cuaucgacaa caagguucuu     2580

gucgcgucca aacaaaauca gaagaaaaga gacgaugugc ccaacaagca gauugugaac     2640

gaacagagaa ucuuuuggaa ucaacucaag gaggccaaac ugauuagccc gaagaaauac     2700

gcuuaucuga ccaagaucga acucacgccu gaggacaaag cccgcuucau ucagcggcag     2760

cuggucgaaa caagacagau caccaagcac guggcuaaca uccuucauca auccuuucac     2820

caggaggagg aaggaacaga uugugacggc guucagauua ucacccugaa ggccacccuc     2880

acaucucagu uccgccaaac guuugggcug uacaaaguga gagagauuaa uccccaucac     2940

caugcgcacg augccuaccu gaacggcuuc aucgcuaaug uccuucugaa gcgguauccu     3000

aaacucgccc ccgaauuugu guacggaaag uauguuaaau acagccuggc ucgcgagaac     3060

aaggccaccg ccaaaaagga guucuauucc aauauucuca aguuucugga aucugacgag     3120

ccuuucugcg augaaaacgg cgagaucuac ugggagaaaa gcuaucaccu ucccagaauc     3180

aagaaagugc uguccagcca ucaggucaau gugguuaaga aaguggaaca gcagaaaggg     3240

ggauucuaca aggagacagu caacucuaaa gaaaagccgg acaaacucau ugagcggaag     3300

aauaacuggg augugaccaa auacggcggg uuuggauccc cugugaucgc uuaugcgauu     3360

gccuucgucu acgcuaaggg caagacacag aaaaagaccc gcgccaucga agggauuacg     3420

aucauggagc aagccgcuuu ugaaaaagac cccacaaccu uccugaagga uaaaggcuuu     3480

ccccagguua ccgaguucau caagcugccu aaguauacac uuuuugaguu cgacaaugga     3540

agacggcgcu uucuggccag ccacaaagaa ucucaaaagg gcaacccguu cauucucucc     3600

gaucagcugg ugacccuccu guaccaugcg cagcacuaug acaaaaucac auaccaggag     3660

agcuuugacu augugaauac gcaucuuucu gauuucagcg cuauucugac cgaaguccuc     3720

gccuuugccg agaaguacac acuggcugac aaaaacaucg aacggauuca agagcuguac     3780

gaggaaaaca aguaugggga gaucuccaug auugcccaga gcuuccuuca gcugcuccag     3840

uucaaugcga ucggagcucc cgccgauuuu aaauucuuug gcgugaccau cccuagaaag     3900

cgcuacacau cucugaccga aauuugggac gccacgauca uuuaucaauc cguuaccggg     3960

cucuacgaga caagaauccg gaugggagau cugugggcug gcgagcagug a              4011


<210>  4126
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4126
augaccaagg acuacacaau cggccuggau auugggacga acagcguggg augggccguc       60

cucaccgacg auuaucagcu gaugaaaaga aagauguccg uucacggcaa uacagagaaa      120

aagaaaauca agaagaacuu cuggggagcu cggcuuuuug acgaagggca aaccgccgag      180

uuccgcagaa caaaacggac caaucgcaga cggcuggcgc gcagaaagua ccggcucucu      240

aaacugcagg aucuuuuugc ugaagaacug ugcaagcagg acgauuguuu cuuugugcgc      300

cucgaagaga gcuuccuggu gcccgaggaa aaacaguaua agccugccuc cauuuuuccg      360

acacuggagg aagagaagga guacuaucaa aaauacccca ccaucuauca ucucagacag      420

aagcuggucg acucuacgga aaaagaggau cuucggcugg uguaccucgc ccuggcucac      480

cuucugaagu accgcggcca uuuccucuuu gaaggggacc uggauaccga gaacacaagc      540

auugaagagu ccuucagagu uuuucuggag caguauucua aacagagcga ccaaccucuc      600

aucgugcacc agcccguccu gaccauccuu acagauaagc uguccaaaac caagaaggug      660

gaagagauuc ucaaauacua uccuacggaa aagaucaaua gcuucuuugc ccagugccug      720

aaacuuauug uuggaaacca ggcuaauuuc aagcggaucu ucgaccugga ggccgaagug      780

aaacuccaau uuucuaagga gacauacgag gaagaccugg agucccugcu cgaaaagauu      840

ggcgaugagu aucuggacau cuuccuucag gcgaaaaagg uccaugaugc cauucugcuc      900

agcgaaauca ucucuuccac cgugaaacac acaaaggcua aacugagcuc uggcauggug      960

gagcgcuacg agagacacaa ggccgaccuu gcuaaguuua aacaguucgu caaggaaaac     1020

guuccccaaa aagccaccgu guuuuucaag gauaccacga aaaaugggua ugcgggauac     1080

auuaagggca agacaaccca ggaggaauuu uacaaauucg ugaagaaaga gcuguccggg     1140

gucgugggaa gcgagccuuu ucucgaaaag aucgaccagg agacauuccu gcugaaacag     1200

cggaccuaua caaacggcgu uauuccgcau caagugcacc ucaucgaacu gaaggccauu     1260

aucgaucagc agaaacagca uuaccccuuu cuugaggaag cugggccuaa gaucauugcc     1320

cuguucaagu uuagaauccc cuauuacguc ggaccucucg cuaaagagca agaggccucc     1380

ucuuucgccu ggauugaacg caagaccgcu gagaaaauca aucccuggaa cuucagcgaa     1440

gugguugaca uugagaaguc cgcgaugcgg uuuauccaga gaaugacgaa acaggauaca     1500

uaucugccga ccgaaaaggu gcugccuaag aauagccuuc ucuaccagaa auauaugauc     1560

uucaacgagc ugaccaaggu cucuuacaaa gacgagcgcg gcgugaagca auacuuuucc     1620

ggcgaugaaa aacagcagau uuucaagcag cuguuucaaa aggagcgggg gaaaaucaca     1680

gucaagaaac uccagaauuu ccuguauacc cacuaccaca uugaaaacgc ccagaucuuu     1740

ggaauugaga aggcuuucaa cgccagcuau ucuacauacc augacuuuau gaaacuugcc     1800

aagacgaauc aaaaggcuau gcaggaaugg cuggagcagc ccgagaugga accuaucuuc     1860

gaggacauug ugaaaauccu caccaucuuu gaagauagac agaugauuaa gcaccaacug     1920

agcaaauauc aggagguuuu cggcgagaag cugcuuaaag aauuugcccg caagcauuac     1980

acaggguggg gacgguucuc cgcgaaacuc auccacggca uuagagaccg caagaccaac     2040

aagacaaucc uggauuaucu gauuaaugac gaugacgugc ccgcuaaccg gaauagaaac     2100

cucaugcagc ugaucaauga ugagcaucuu ucuuucaaag aagagaucgc caaggccacc     2160

gucuuuagca aacacaaguc ccugguggac guuauucagg aucuccccgg gagcccugcu     2220

aucaaaaagg gaauuuggca aucucugaag aucguggagg agcugauugc caucaucggc     2280

uacaaaccga agaacauugu caucgagaug gcucgcgaaa aucagaaaac gcaccggacc     2340

ucccccagac uuaaggcccu cgagaacggc cugaaacaga uugggagcac acugcucaag     2400

gaacagccua ccgacaauaa ggcgcugcaa aaagagcggc uuuaccugua uuaccuccag     2460

aacggacgcg auauguauac aggcgaaccc cuggagaucg agaaucugca ucaguacgaa     2520

guggaccaca uuaucccuag aucuuucauc guggauaacu ccauugacaa uaagguucuu     2580

gucgccagca aacaaaacca gaagaaacgg gacgaugugc ccaagaagca gaucgugaac     2640

gagcagagaa uuuucuggaa ucaacucaaa gaagcuaagc ugaucucccc gaaaaaguau     2700

gccuaccuga ccaaaauuga gcucacgccu gaagacaagg cucgcuucau ccagcggcag     2760

cuggucgaga caagacagau uaccaagcau guggccaaca uccuucacca aucuuuuaau     2820

caggaggaag aggggacaga uugugacgga guucagauca uuacccugaa agcgacccuc     2880

acaagccagu uccgccaaac guuuggccug uauaagguga gagaaaucaa cccccaccau     2940

cacgcccaug augcuuaccu gaauggguuc auugccaacg uccuucugaa acgguacccu     3000

aagcucgcuc ccgaguuugu guauggcaaa uacguuaagu auucccuggc ccgcgagaau     3060

aaagccaccg cuaagaagga guucuacucu aacauccuca aauuucugga aagcgacgaa     3120

ccuuucugcg augagaaugg agaaauuuau ugggagaagu cccaccaucu ucccagaauc     3180

aaaaaggugc ugagcucuca ccaggucaac gugguuaaaa agguggagca gcagaagggc     3240

ggguucuaca aagaaacagu caauuccaag gagaaaccgg acaagcucau cgaacggaaa     3300

aacaauuggg augugaccaa guauggaggc uuugggagcc cugugauugc guacgccauc     3360

gcuuucgucu acgccaaggg aaaaacacaa aagaaaaccc gcgccauuga gggcaucacg     3420

auuauggaac aggcugccuu ugagaaggac cccacaaccu uccugaaaga gaagggguuu     3480

ccccagguua ccgaauuuau caagcugccu aaauauacac uuuuugaguu cgauaacggc     3540

agacggcgcu uucuggcguc ucacaaggaa ucccaaaaag gaaauccguu cauccucagc     3600

gaccagcugg ugacccuccu guaccaugcu cagcacuaug auaagauuac auaccaggag     3660

ucuuuugacu augugaacac gcaucuuagc gacuucuccg ccauccugac cgaaguccuc     3720

gccuuugcug agaaauacac acuggccgau aagaacauug agagaaucca agaacuguau     3780

gaggaaaaua aauacggcga gaccagcaug auugcgcagu cuuuccuuca gcugcuccag     3840

uucaacgcua ucggggcccc cgccgacuuu aaguucuuug gagugacaau uccucggaag     3900

cgcuacaccu cccugacgga gaucugggau gcuaccauca uuuaucaaag cguuacaggc     3960

cucuacgaaa ccagaauccg gaugggggac cugugggccg gagagcagug a              4011


<210>  4127
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4127
augaagaaca ugaaaaagcc cuacaccauc ggccuggaca uugggacaaa uagcguggga       60

ugggccgucc ucacggauca guaugaccug guuaaaagaa agaugaaaau cgcuggcgau      120

uccgagaaga agcaaauuaa aaagaacuuc uggggagugc ggcuuuuuga cgaagggcag      180

accgccgcgg aucgcagaau ggcucggaca gcccgcagac ggaucgagcg cagacggaau      240

cgcauuucuu accugcaggg caucuucgcc gaagagauga gcaaaaccga cgcuaacuuu      300

uucugcagac ucuccgauuc uuuuuaugug gacaaugaaa agcggaacag ccgccacccu      360

uucuuugcca caaucgagga ggaagucgag uaccauaaaa auuauccgac cauuuaccac      420

cugagagagg aacuugugaa cuccucugag aaggcugauc ugcggcucgu uuaucuggcc      480

cuggcgcaua ucauuaagua ccgcgggaau uuccucaucg aaggagcccu ggacacacag      540

aacaccagcg uggauggcau uuacaaacaa uuuauccaga cguauaauca ggucuucgcu      600

uccggcauug aggacgggag ccuuaagaaa cuggaagaua acaaggacgu ggccaaaauc      660

cucguugaga aggugaccag aaaagaaaag cuggagcgga uccuuaagcu guaccccgga      720

gagaaaucug cuggcauguu ugcccaguuc auuucccuca ucgucgggag caagggaaau      780

uuucaaaaac cuuucgaccu gauugaaaag ucugauaucg agugugcgaa agacuccuau      840

gaagaggauc uggaaagccu ccuggcccuu auuggcgacg aguacgcuga gcuguucgug      900

gccgcuaaga acgccuauuc ugccgugguc cucuccagca ucaucacagu ugcugaaacc      960

gagacaaaug cgaagcuguc cgccucuaug auugaaagau uugauaccca cgaggaagac     1020

cuuggggagc ugaaggcuuu caucaaacuc caucugccca aacacuacga ggaaauuuuu     1080

agcaacacgg agaagcacgg auaugccggc uacaucgaug gcaaaacaaa gcaggccgac     1140

uucuacaagu auaugaaaau gacccuggaa aacauugagg gggcugauua cuuuaucgcc     1200

aagaucgaga aagaaaauuu ccuccgcaag cagcggacau uugacaacgg agcgauuccu     1260

caucagcugc accuugagga acuggaggcu auccuccauc aacaggccaa auauuacccc     1320

uuccugaagg aaaauuauga uaagauuaaa ucccuuguga ccuuuagaau cccuuacuuc     1380

gugggcccgc uggccaacgg gcagagcgag uuugcuuggc ucacccgcaa ggccgacgga     1440

gagauucggc ccuggaauau cgaagagaaa gucgacuucg gcaagucugc uguggauuuu     1500

auugagaaaa ugacaaacaa ggacacguau cugccuaaag aaaauguucu gcccaagcac     1560

ucccucugcu accaaaagua ccugguguau aacgaacuua ccaaagucag auacaucaau     1620

gaucagggga agacaagcua uuucucugga caggagaaag agcagaucuu caacgaccug     1680

uuuaagcaaa aacgcaaggu gaagaaaaag gaucucgaac uguuccugcg gaauaugagc     1740

cacguugagu ccccuaccau ugaaggccuu gaggacucuu uuaacagcuc cuacagcaca     1800

uaucaugauc uccugaaagu gggcaucaag caggaaauuc uggacaaucc cgucaacacc     1860

gagaugcucg agaauaucgu gaaaauucug acggucuucg aagauaagag aaugaucaag     1920

gagcagcuuc agcaauuuuc ugacgugcug gaugggguug ugcucaaaaa gcuggaacgc     1980

cggcacuaca caggaugggg cagacugucc gccaaacuuc ucauggggau ccgcgacaag     2040

cagagccauc ugaccauucu ggauuaucuc augaacgacg acggacugaa ccggaaucuu     2100

augcagcuga ucaacgauuc uaaucucucc uucaaaagca uuaucgagaa ggagcagguc     2160

accacagcgg acaaggauau ucaauccauc guggccgacc uggcuggcuc uccggccauc     2220

aaaaagggga uucugcagag ccuuaaaauc guugaugaac ucguguccgu caugggcuac     2280

ccuccccaga ccauuguggu ggagauggcu agagaaaacc agacaacggg aaagggcaaa     2340

aauaacucuc ggccucgcua caagagccug gagaaagcca ucaaggaauu ugggucccaa     2400

auucugaagg agcaccccac cgacaaucag gagcucagaa acaaucggcu guaucuuuac     2460

uaucugcaga acggaaaaga uauguacaca ggccaagacc ucgauaucca uaaucugagc     2520

aacuaugaca ucgaucacau uguuccccag ucuuucauca ccgacaauuc cauugauaac     2580

cugguccuua caagcucugc ggggaauaga gaaaagggag acgacgugcc uccgcucgag     2640

aucgugcgca aacggaaggu cuuuugggaa aaacuguacc agggcaaccu gauguccaag     2700

agaaaguucg auuaucucac caaagccgag cgcgggggcc ugacggaagc ugacaaggcc     2760

agauuuauuc accggcagcu uguggagacc cgccaaauca ccaaaaacgu ugcuaauauu     2820

cugcaucaga gauucaacua cgagaaggau gaccacggaa auacaaugaa acaggugcgg     2880

aucgucacac ucaagagcgc ccuggugucu caguuucgca agcaauucca gcuguacaaa     2940

guuagagaug ugaacgacua ucaucacgcc cacgaugcuu accuuaaugg cgucguggcg     3000

aacacccugc ucaagguuua uccccagcug gaaccugagu ucguguacgg ggacuaucau     3060

caguuugauu gguucaaagc caauaaggcu acggccaaaa agcaauuuua cacaaacauc     3120

augcuguucu uugcccagaa ggaccggauu aucgaugaaa auggagagau ucucugggac     3180

aaaaaguauc uugacaccgu caaaaaggug augagcuacc gccagaugaa caucgugaaa     3240

aagacagaaa uucaaaaggg cgaguucucc aaagcuacca ucaagcccaa agggaauagc     3300

ucuaagcuga ucuccagaaa aaccaacugg gauccuauga aguacggagg ccucgacagc     3360

cccaauaugg ccuaugcggu cguuauugag uacgcuaaag ggaagaacaa gcugguguuu     3420

gagaaaaaga ucauucgggu gacaaucaug gaacgcaaag ccuucgaaaa ggaugagaaa     3480

gccuuucugg aggaacaggg cuauagacag ccgaaggucc uugcuaagcu gccuaaauac     3540

acgcucuaug agugugaaga gggacggaga cgcaugcugg ccuccgcgaa ugaagcucag     3600

aagggcaacc aacaggugcu ccccaaccac cugguuaccc uucugcauca cguggccaau     3660

ugcgaggucu cugacgggaa aagccucgau uacauugagu ccaaccggga aauguucgcc     3720

gagcugcugg cucauguguc ugaauuugcc aagagauaua cacuugcuga ggccaaucug     3780

aacaaaauca aucaacucuu cgaacagaac aaggagggag acaucaaggc gauugcccag     3840

agcuucguug aucugauggc uuuuaaugcc augggcgcuc cugccuccuu caaauuuuuc     3900

gagaccacaa ucgaacgcaa gcgguacaac aaucucaaag agcugcuuaa cucuaccauu     3960

aucuaccaga gcauuacggg gcuguaugaa uccagaaagc gccuggacga uuga           4014


<210>  4128
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4128
augaagaaau ucgagaacua cuaucugggc cucgacaucg ggaccagcuc cauuggaugg       60

gccgugacaa auucucagua cgauauccug aaguuuaacg gcaaauauau gugggggacg      120

agacuuuucc ccgaagcuaa uaccgcccaa gagcggcgca uucacagaag cucccggcgc      180

agacugaagc ggcgcaaaga aagaauccag auucuccaga ugcuguuuga caaggagauc      240

gcgaagaucg auucuggauu cuuucagcgg cuuaaagaca gcaaguacua uaaagaagau      300

aagacagaga aacaaaccaa cuccauuuuc caugacaagg auuacucuga caaggaguau      360

caccaggauu uuccuacaau cuaccaucug cgcaaauucc uccuggaagg caauaagccg      420

aaagacauua gauuugucua ccuggcucuc caccauaucc ugacccaccg ggggcacuuc      480

cuuuuucccg auauggaggu uagcaacgug acagaguucu ccaauauuuu uagcgaacug      540

aagcaguauc ucuacgacga gauggaucug gacuucgaau ggaaaaacgg aaaacuuaau      600

gagguggaaa acauccugaa agaccgcaau cucgccaagu cugagaagga aaagaagcug      660

ugcggcauua ucgagcugga gggcaaaauc gauaaccaga gaaaggccau gauugggaug      720

auguguggau gcaaaaagaa guucuccgac cucuuucaaa augaaaacua ucgggagcug      780

gaaaucagcu cuuucuccuu ugcugauagc ucuuacgagg aaaaacgcga cgagcuugag      840

ggcauucugg ccgaaaauau ccucugucug gauuaucuua agaccauuua cgacuggggg      900

aaacuguccg auauccucca gggagaggaa agcaucuccg ucgcuaaggu gaaaucuuau      960

gaggaacauc agcuggaccu gaaguaccuc aagaacauuc ugaaacuuua cagccacaag     1020

gagaaagaga acguuuucag aaagaaagaa ggcaaguauc cuaaguacau cuccgggaaa     1080

cugagccagg aggaauuuaa uaagucuauu aaaaagaucc ucgaggagau uaaauccgug     1140

aagcaagaag aucggaaagu cuucgacacg cugcuuaaga gagccagcaa caaucugcuc     1200

ugccccaaac aggugaucaa agagaacgga guuaucccuu aucagauuca uaaguuugaa     1260

cuggagaaaa uccugcgcaa uauggcggau uucuuuccca ugcucaagga aaagaaggac     1320

ggcaagaccc ugucugagaa aauuaucagc guguucacau uucggauucc uuacuauguc     1380

ggcccgcuua accaaaauuc cgacagagcc uggcugguga agaacaaaga ugagaagauc     1440

uaccccugga auuucgaaga gauugugaac cucgaagagu cugcugaaaa auuuauccag     1500

aaucugacca acaaguguac auaucugguc cuugaggacg uucucccuaa gagcuccauc     1560

cuguacagca aauucauggu gcugaaugag cucaacaauc ugaagauuga uggggaagcc     1620

aucucugugg accuuaaaca gaagauuuac cugaaccucu uccagaaaua uaagaagguc     1680

acccugaaaa agcugaaagg auaccuuaag uccgagaaca uccucauuga uacgagcaca     1740

caaaucaccg gcaucgacgg ggauuuuaaa ucuucccugg gaagcuaucu ggacuucuac     1800

aauauucucg gcgauaaggu gaaaacagac uuugggaaga agcugaucga aaacugcauu     1860

cuuuggauca cccuguauac cggagagaaa aagcuccuga aaaauaagau uaucgcuaac     1920

uacaaaggcg aacuguccga ggaggaaauc aagaagauug uuaaucuuaa auauaaggau     1980

uggggccgcc ucucuuacgc cuuccuggag gaaauccaga gcgcgucccu ggagacaggg     2040

gagcuccgga acauuaucca gaugaugugg gaaacgaaua acaaucugau ggagcuucug     2100

ucuagcaacu accaguuucu cuccgaaauu gagaaaagaa auagcguggu cgccaucgga     2160

aaggaguuca acuaugaaac cauccugggc gacucuuacg cuucccccag cgugaaacgc     2220

augauuuggc aaucucuguc cguuguggau gagaucaaga agauuaugaa aaaggccccu     2280

aaaaagaucu uuauugaaau ggcucggcag gaggacauga aaaaggaaag aaaggagagc     2340

cgcaaaucua cauuccuuac ccucuauaag agcaucaaag aagaggggcg ggacuggauu     2400

aaggagaucg aaaauugguc cgauagcgag uuuagaucua aaaagcugua ccuguauuac     2460

acacagaugg gaaaguguau guauaccggc gaaaagaucu cccucgacca gcuguucaac     2520

aagaauauuu acgauaucga ccacauuuac ccccgcagca aaacgaagga ugacuccauc     2580

gagaacauug uccuugugaa acggaacauc aaugccaaga aaacagauga auauccgcug     2640

gagagaaaca uccaacagaa gcagcacgac uuuuggaaga ugcuccauuc uaaaaagcug     2700

auuggggaua aaaaguacga gcggcugacc cgcaccacag aguucaccga cgaagaacuu     2760

agcgauuuua ucgccagaca gcuggucgag acacggcaau ccacgaaaau uguggcugac     2820

auccucaaga aucuguuccc ugagaccaag auuguuuaug ugaaagcgaa ccucacaucu     2880

gauuucagaa agaauuuuaa aauccuuaag agccgcgaca ucaacgacua ccaccaugcc     2940

cacgaugcuu aucugaauau ugucaccggc aacguguaca auaucaaauu cacagacaac     3000

ccccggaauu uuauuaagga uaagaaacug gaaggaaaga aauauaaccu caagguugag     3060

aaaaucuucg aauacagaaa ggagaaugaa uccuuuugga acccuaagac cauggugacg     3120

aaaauugaga auuacaucuu caagaaacgc ccccaguuua ccagauauuc uuucgagcag     3180

cauggcgggu uuuucgacca aaacauuguc gauaaggaaa gcugcaaaaa gggaacaggc     3240

uacaucccca ucaaauccag caacgccauu cuguauaaua ccucuaagua cgggggauau     3300

ggcuccguga cagggaccua cuuuuuccug guggagcaca cgaugaaggg caaacggauc     3360

cgcacaauug aaaugaugcc ucuuuaucug agcaagaaaa uccacucuaa ggaggaacuc     3420

gagaaauacu guaaggagaa gcuggaacuc cagaaaccgu ccguuagacu ggcccggauu     3480

aaguacaaca gccuucugaa aaucaaugga uuccccuauc auaucaccgg caagacaaac     3540

gacucuuacu ggauuauguc cgcuauccag cuccugcuga gcaaaaauua uuacgaguau     3600

cuucgcaagc uguacaucuu uugcaaggaa gagagacucg aagaggagau ugucggggag     3660

aaaaacauua agcuguauac cuguauccuc gaaaaacugg aaaaguccau cuacagccac     3720

aaacuucuga auauuaacua caauggaaag ucuaagaacc ugaaagauau ccucgagagc     3780

gagaaggaaa auuuccugcu ucuguccgag aaaaagcagg cccaaauucu caucgaaauu     3840

uuuacccugc ucgagucuaa caauuucggc gcgaaccugg agagcuuugg gugcgggaaa     3900

aagugcggca ucacaaaaau uaauaagaac aucgacaaac uugaagaggu gcugcugauc     3960

aaucaguccc cuacgggacu cuucgaaaac ucuauugauc ugaaaaaggu guga           4014


<210>  4129
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4129
augaagaaag acuacgugau cggccuggau auugggacca acagcgucgg augggccguu       60

augacagagg acuaucagcu cgugaagaaa aagaugccca ucuacggcaa uacggagaaa      120

aagaagauua aaaagaacuu cuggggagug agacuguuug aggaagggca caccgcugag      180

gaucggcgcc uuaaaagaac agcccggcgc agaaucuccc ggcgcagaaa ucggcugcgc      240

uaucuccaag cguucuuuga agaggcuaug accgaccugg augagaacuu cuuugccaga      300

cuucaggaau cuuuccuggu cccugaggac aagaaauggc aucggcaccc gauuuuugcc      360

aagcucgaag augagguggc uuaccaugag acauauccca ccaucuacca ucugcgcaag      420

aaacuggccg acagcuccga acaggcugau cucagacuga ucuaucuugc ccuggcgcac      480

auuguuaagu accggggcca cuuccucauc gaggggaaac ugucuacaga aaauauuagc      540

gugaaggagc aguuucaaca guucaugauc auuuacaacc agaccuuugu caauggagaa      600

ucccgccuug ugucugcccc ucugcccgag agcguucuca ucgaggaaga acugacggaa      660

aaagccucca gaaccaagaa aagcgagaag gugcugcagc aauucccuca ggaaaaggcu      720

aacggccucu uuggccaguu ccugaaacuu auggucggga auaaggcuga cuucaaaaag      780

guguuuggac uggaggagga agccaaaauu acauaugcgu cugaguccua cgaagaggau      840

cucgaaggca uccuggccaa ggugggggac gaguauagcg augucuuccu ugcugccaag      900

aacguuuacg acgcugugga gcugucuacc auccucgccg acuccgauaa aaagagccac      960

gccaaacugu cuuccagcau gauugugcgg uuuacagaac aucaggagga ccugaagaaa     1020

uucaagagau uuauccgcga aaauugcccc gaugaguaug acaaccucuu caagaaugag     1080

caaaaagaug gauacgcugg cuauauugcg cacgccggga aggucuccca gcugaaauuu     1140

uaccaguacg ugaagaaaau cauucaagac aucgcuggag ccgaauauuu ccuugagaag     1200

aucgcccagg aaaacuuucu gcggaagcag agaaccuucg auaauggcgu uauuccucau     1260

cagauccacc ucgcugagcu gcaagccauu auccaccgcc aggcggcuua cuauccguuu     1320

cuuaaagaaa accaggagaa gauugagcag cuggugacgu uccggauccc cuacuauguc     1380

ggcccucucu cuaaagggga cgccagcaca uuugccuggc ugaagagaca auccgaggaa     1440

cccauccgcc cuuggaaccu gcaggaaacc guggaucucg accagagcgc uacagccuuc     1500

auugagcgga ugaccaauuu cgauaccuac cugcccucug aaaaaguucu uccgaagcau     1560

ucccugcucu augagaaauu uaugguguuc aacgagcuga caaagaucag cuacacggac     1620

gacagaggaa uuaaggcuaa uuuuucuggc aaagaaaagg agaaaaucuu cgauuaccug     1680

uuuaagaccc gccggaaagu caagaagaaa gacauuaucc aguucuauag aaacgaauac     1740

aauacagaga uugugacccu uagcgggcuc gaagaggauc aauuuaacgc cuccuucucu     1800

acauaucagg accugcugaa guguggacuc acccgcgcgg agcuggauca cccugacaau     1860

gccgaaaaac uugaggauau caucaagauu cugacgaucu uugaagaccg gcagcggauu     1920

agaacacagc ucagcaccuu caaaggccaa uuuuccgcug agguccugaa gaagcuggag     1980

cgcaaacauu acaccgggug gggaagacuu agcaagaaac ucaucaacgg cauuuaugau     2040

aaggaaucug gcaaaacaau ccugggguac cugaucaagg acgauggagu guccaagcac     2100

uauaaucgga acuucaugca gcucauuaau gacagccagc ugucuuuuaa aaacgccauc     2160

caaaaggcuc aguccagcga gcaugaagag acccuuuccg aaacaguuaa ugagcuggcc     2220

ggcucucccg cgauuaaaaa ggggaucuac cagagccuca aaauugugga ugagcugguc     2280

gccaucaugg gauacgcucc uaagagaauc gugguugaaa uggcccgcga gaaccagacg     2340

accuccacag gcaaacggag aucuauucaa cgccugaaga ucguggaaaa ggcuauggcc     2400

gagauuggga gcaaucuucu caaagaacag cccaccacaa acgagcagcu gagagacacc     2460

cggcuguucc ucuauuacau gcagaacggc aaggacaugu auacgggaga ugagcugucc     2520

cuucaccgcc ugagccacua cgacaucgau cauauuaucc cccaaucuuu caugaaagac     2580

gauucccucg acaaucuggu ccuggugggc agcaccgaaa acagagggaa gucugaugac     2640

gugccuucca aagagguugu caaggauaug aaggccuauu gggaaaaacu uuacgcugcg     2700

ggacucauca gccagcggaa guuucagcgc cugacaaaag gcgagcaagg gggacugacc     2760

cucgaagaca aggcccacuu cauucagaga cagcuggugg agacacggca gaucaccaaa     2820

aauguggcug gcauucuuga ucaacgcuau aacgccaauu cuaaggagaa gaaaguccag     2880

aucauuacgc ugaaggccag ccucacaucc caguuuagaa gcaucuucgg gcuguacaaa     2940

gugcgggaag uuaacgacua ccaucacggc caggaugcuu aucugaauug cguggucgcc     3000

accacacuuc ugaaggugua cccgaaccuc gcgcccgagu uuguuuaugg agaauacccu     3060

aaauuccaaa ccuuuaagga gaauaaggcu accgccaaag ccauuaucua uacaaaccug     3120

cuccgcuucu uuacggagga cgaacccaga uucaccaagg acggcgagau ccuguggucu     3180

aauuccuacc uuaaaacaau uaagaaagaa cugaacuauc accagaugaa uaucgugaag     3240

aaggucgagg ugcagaaagg gggauuuagc aaggaaucca uuaaaccuaa gggccccucu     3300

aacaaacuca ucccgguuaa gaaugggcug gauccucaga aauacggagg cuucgacagc     3360

cccauugugg cuuacaccgu ccuguucaca caugagaagg ggaagaaacc ucuuaucaag     3420

caagagaucc ugggcauuac caucauggag aaaacgcggu uugaacagaa ccccauucuc     3480

uuccuggaag agaagggauu ucucagaccu cgcgugcuga ugaaacuucc caaguauaca     3540

cuguacgagu ucccggaagg ccggagacgc cuccuggccu ccgcgaagga ggcucagaaa     3600

gggaaccaga uggugcugcc ugaacaccuu cugacccucc uguaucaugc caagcaaugu     3660

cuccugccca aucagucuga gagccuuacc uacgucgaac agcaccagcc cgaguuucaa     3720

gaaauccugg agcggguugu ggauuucgcc gaggugcaua cacuggcuaa auccaagguc     3780

cagcagauug ugaaacucuu ugaagccaac caaaccgcug acguuaagga gaucgccgcg     3840

ucuuucaucc agcugaugca guuuaaugcc augggagcuc cuagcacauu caaguuuuuc     3900

cagaaagaua uugaaagagc ccgcuauacg uccaucaagg agauuuuuga cgcuaccauc     3960

auuuaccaaa gcacaaccgg ccuuuaugag acacggagaa aaguggucga uuga           4014


<210>  4130
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4130
augaagaaag acuacgugau cggccuggau auugggacca acagcgucgg augggccguu       60

augacagagg acuaucagcu cgugaagaaa aagaugccca ucuacggcaa uacggaaaag      120

aagaagauua aaaagaacuu cuggggagug agacuguuug aggaaggaca caccgcugag      180

gaucggcgcc uuaaaagaac agcccggcgc agaaucuccc ggcgcagaaa ucggcugcgc      240

uaucuccaag cguucuuuga agaggcuaug accgaccugg augagaacuu cuuugccaga      300

cuucaggaau cuuuccuggu cccugaggac aagaaauggc aucggcaccc gauuuuugcc      360

aagcucgaag augagguggc uuaccaugag acauauccca ccaucuacca ucugcgcaag      420

aaacuggccg acagcuccga acaggcugau cucagacuga ucuaucuugc ccuggcgcac      480

auuguuaagu accggggcca cuuccucauc gaggggaaac ugucuacaga aaauaccagc      540

gugaaggacc aguuucaaca guucaugguc auuuacaacc agacguuugu gaauggagag      600

ucccgccuug uuucugcccc ucugcccgaa agcgugcuca ucgaggagga acugaccgag      660

aaagcuagca gaacaaagaa aagcgaaaag guccugcagc aauucccuca ggagaaggcc      720

aacggccucu uuggccaguu ccugaaacuu auggugggga auaaggcuga uuucaaaaag      780

guguuuggac uggaagagga ggccaaaauu accuaugcgu cugaauccua cgaggaagac      840

cucgagggca uccuggccaa ggucggggau gaauauagcg acguuuuccu ugcugccaag      900

aacguguacg acgcugugga gcugucuaca auucucgccg auuccgacaa aaagagccac      960

gccaaacugu cuuccagcau gaucguccgg uuuaccgagc aucaggaaga ucugaagaaa     1020

uucaagagau uuauccgcga gaauugcccc gacgaauaug auaaccucuu caagaaugag     1080

caaaaagacg gauacgcugg cuauauugcg cacgccggga agguguccca gcugaaauuu     1140

uaccaguacg uuaagaaaau cauucaagau aucgcuggag ccgaguauuu ccuugaaaag     1200

auugcccagg agaacuuucu gcggaagcag agaacguucg acaauggcgu gaucccucau     1260

cagauccacc ucgcugaacu gcaagccauu auccaccgcc aggcggcuua cuauccguuu     1320

cuuaaagaga accaggaaaa gauugagcag cuggucacau uccggauccc cuacuaugug     1380

ggcccucucu cuaaagggga ugccagcacc uuugccuggc ugaagagaca auccgaggaa     1440

cccauucgcc cuuggaaccu gcaggagaca guugaccucg aucagagcgc uaccgccuuc     1500

aucgaacgga ugaccaauuu cgacacauac cugcccucug agaaagugcu uccgaagcau     1560

ucccugcucu augaaaaauu uauggucuuc aacgagcuga cgaagaucag cuacaccgac     1620

gauagaggaa uuaaggcuaa uuuuucuggc aaagagaagg agaaaaucuu cgacuaccug     1680

uuuaagacac gccggaaagu gaagaagaaa gauauuaucc aguucuauag aaacgaauac     1740

aauaccgaaa uugucacacu uagcgggcuc gaggaagacc aauuuaacgc cuccuucucu     1800

accuaucagg aucugcugaa guguggacuc acgcgcgcgg agcuggacca cccugauaau     1860

gccgagaaac uugaagacau cauuaagauc cugacaaucu uugaggaucg gcagcggauu     1920

agaacccagc ucagcaccuu caaaggccaa uuuuccgcug aagugcugaa gaagcuggag     1980

cgcaaacauu acacagggug gggaagacuu agcaagaaac ucaucaacgg cauuuaugac     2040

aaggagucug gcaaaaccau ccuggauuac cugauuaagg acgauggggu uuccaagcac     2100

uauaaucgga acuucaugca gcucaucaau gacagccagc ugucuuuuaa aaacgccauc     2160

caaaaggcuc aguccugcga acaugaggaa acacuuagcg agacggugaa ugaacuggcc     2220

ggaucccccg cgauuaaaaa gggcaucuac cagucucuca aaauugucga cgagcuggug     2280

gccaucaugg gguacgcucc uaagagaauu guuguggaga uggcccgcga aaaccagacc     2340

acaagcaccg gaaaacggag auccauccaa cgccugaaga ucgucgagaa ggcuauggcc     2400

gaaauuggcu cuaaucuucu caaagagcag cccacaacca acgaacagcu gagagauacg     2460

cggcuguucc ucuauuacau gcagaacggg aaggacaugu auaccggcga ugagcugagc     2520

cuucaccgcc ugucccacua cgacaucgau cauauuaucc cccaauccuu caugaaagac     2580

gauucucucg acaaucuggu gcugguggga uccacagaga acagaggcaa gagcgaugac     2640

guuccuucua aaaaggucgu gaaggauaug aaagccuauu gggaaaaacu uuacgcugcg     2700

gggcucauuu cccagcggaa auuucagcgc cugaccaagg gagagcaggg cgggcugaca     2760

cucgaagaca aagcccacuu cauccaaaga cagcuggugg agacccggca gaucacgaag     2820

aaugucgcug ggauucuuga ucaacgcuau aacgccaaga gcaaagaaaa gaaagugcag     2880

aucauuacac ugaaggccuc ucucaccagc caguuuagau ccaucuucgg ccuguacaaa     2940

guucgggagg ugaaugacua ccaucacggg caggacgcuu aucugaacug ugucguggcc     3000

acaacccuuc ugaagguuua cccgaaucuc gcgcccgagu uuguguaugg cgaauacccu     3060

aaguuccagg cuuuuaaaga gaacaaggcc accgccaaaa caauuaucua uacgaaucug     3120

augcgcuucu uuaccgaaga ugagcccaga uucacaaagg acagcgagau ucucuggucu     3180

aacuccuacc ugaaaaccau caagaaggaa cuuaauuauc accaaaugaa caucgucaaa     3240

aagguggagg uucagaaagg aggcuuuagc aaggaaucca uuaaaccuaa ggggcccucu     3300

aauaaacuga ucccggugaa gaacggacuc gauccucaga aguacggcgg guucgacagc     3360

cccgucgugg cuuacacagu gcuguucacc caugagaaag gaaagaaacc ucugauuaag     3420

caagaaaucc uuggcauuac gaucauggag aaaacacggu uugagcagaa ucccauccug     3480

uuccucgaag agaagggguu ucugagaccu cgcguccuca ugaagcugcc caaauauacc     3540

cuuuacgagu ucccggaagg ccggagacgc cugcucgccu ccgcgaagga agcucagaaa     3600

ggaaaccaga ugguucugcc ugagcaucug cuuacccugc ucuaucacgc caagcaaugc     3660

cugcucccca accagucuga gagccuggcc uacguggaac agcaccagcc cgaguuucaa     3720

gaaauucuug agcggguggu cgauuucgcu gaagugcaua cacuggccaa auccaagguu     3780

cagcagaucg ugaagcuguu ugaggcuaau caaaccgccg acgucaaaga gauugcggcc     3840

ucuuucaucc agcucaugca guuuaacgcu augggcgccc cuagcacauu caaguuuuuc     3900

cagaaagaua uugaaagagc ucgcuauacg uccaucaagg agaucuuuga cgccaccauu     3960

aucuaccaaa gcacaaccgg gcuguaugaa acacggagaa aagugguuga uuga           4014


<210>  4131
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4131
augaagaaag acuacgugau cggccuggau auugggacca acagcgucgg augggccguu       60

augacagagg acuaucagcu cgugaagaaa aagaugccca ucuacggcaa uacggaaaag      120

aagaagauua aaaagaacuu cuggggagug agacuguuug aggaaggaca caccgcugag      180

gaucggcgcc uuaaaagaac agcccggcgc agaaucuccc ggcgcagaaa ucggcugcgc      240

uaucuccaag cguucuuuga agaggcuaug accgcccugg acgagaacuu cuuugccaga      300

cuucaggaau cuuuccuggu cccugaggau aagaaauggc aucggcaccc gauuuuugcu      360

aagcucgaag acgagguggc cuaccaugag acauauccca ccaucuacca ucugcgcaag      420

aaacuggcug auagcuccga acaggccgac cucagacuga ucuaucuugc gcuggcccac      480

auuguuaagu accggggcca cuuccucauc gaggggaaac ugucuacaga aaauauuagc      540

gugaaggagc aguuucaaca guucaugauc auuuacaacc agaccuuugu caauggagaa      600

ucccgccuug ugucugcucc ucugcccgag agcguucuca ucgaggagga acugacggaa      660

aaagccucca gaaccaagaa aagcgagaag gugcugcagc aauucccuca ggaaaaggcu      720

aacggccucu uuggccaguu ccugaaacuu auggucggga auaaggccga uuucaaaaag      780

guguuuggac uggaggagga agcgaaaauu acauaugccu cugaguccua cgaagaggac      840

cucgaaggca uccuggcuaa ggugggggau gaguauagcg acgucuuccu ugccgcuaag      900

aacguuuacg augccgugga gcugucuacc auccucgccg acuccgacaa aaagagccac      960

gcuaaacugu cuuccagcau gauugugcgg uuuacagaac aucaggagga ucugaagaau     1020

uucaaaagau uuauccgcga aaacugcccc gacgaguaug auaaucucuu caagaacgag     1080

caaaaggacg gauacgcggg cuauauuacc cacgccggga aagucuccca gcugaaguuu     1140

uaccaguacg ugaaaaagau cauucaagau aucgcuggag ccgaauauuu ccuugagaaa     1200

aucgcccagg aaaauuuucu gcggaagcag agaacguucg acaacggcgu uauuccucau     1260

cagauccacc ucgcugagcu gcaagccauu auccaccgcc aggcggcuua cuauccguuu     1320

cuuaaggaaa accaggagaa aauugagcag cuggugacau uccggauccc cuacuauguc     1380

ggcccucucu cuaaggggga ugccaauacc uuugccuggc ugaaaagaca aagcgaggaa     1440

cccauccgcc cuuggaaccu gcaggaaaca guggaccucg aucaguccgc uaccgccuuc     1500

auugagcgga ugaccaauuu cgacacauac cugcccagcg aaaagguucu uccgaaacau     1560

ucucugcucu augagaaguu uaugguguuc aacgagcuga cgaaaaucuc cuacaccgau     1620

gacagaggaa uuaaggcuaa uuuuagcggc aaggaaaaag agaagaucuu cgacuaccug     1680

uuuaaaacac gccggaaggu caaaaagaag gauauuaucc aguucuauag aaacgaauac     1740

aauaccgaga uugugacacu uucugggcuc gaagaggacc aauuuaacgc cagcuucucc     1800

accuaucagg aucugcugaa auguggacuc acgcgcgcgg agcuggacca cccugauaau     1860

gccgaaaaac uugaggacau caucaaaauu cugacaaucu uugaagaucg gcagcggauu     1920

agaacccagc ucucuaccuu caagggccaa uuuagcgcug agguccugaa aaagcuggag     1980

cgcaagcauu acacagggug gggaagacuu uccaaaaagc ucaucaacgg cauuuaugac     2040

aaagaaagcg gcaagaccau ccuggauuac cugaucaaag acgauggggu gucuaagcac     2100

uauaaucgga acuucaugca gcucauuaau gacucccagc ugagcuuuaa gaacgccauc     2160

caaaaagcuc agucuuccga gcaugaagag acacuuagcg aaacgguuaa ugagcuggcc     2220

ggaucccccg cgauuaagaa aggcaucuac cagucucuca agauugugga ugagcugguc     2280

gccaucaugg gguacgcucc uaaaagaauc gugguugaaa uggcccgcga gaaccagacc     2340

acaagcaccg gaaagcggag auccauucaa cgccugaaaa ucguggaaaa ggcuauggcc     2400

gagauuggcu cuaaccuucu caaggaacag cccacaacca augagcagcu gagagacacg     2460

cggcuguucc ucuauuacau gcagaacggg aaagacaugu auaccggcga ugagcugagc     2520

cuucaccgcc ugucccacua cgacaucgau cauauuaucc cccagagcuu caugaaggac     2580

gauucucucg acaaucuggu ccugguggga uccacagaaa acagaggcaa aagcgaugac     2640

gugccuucua aggagguugu caaagauaug aaggccuauu gggaaaaacu uuacgcugcg     2700

gggcucaucu cccaacggaa auuucagcgc cugaccaagg gagagcaggg cgggcugaca     2760

cucgaagaca aagcccacuu cauucaaaga cagcuggugg agacccggca gaucacgaag     2820

aauguggcug ggauucuuga ucaacgcuau aacgccaaaa gcaaggagaa gaaaguccag     2880

aucauuacac ugaaggccuc ucucaccagc caguuuagau ccaucuucgg ccuguacaaa     2940

gugcgggaag uuaaugacua ccaucacggg caggaugcuu aucugaacug cguggucgcc     3000

acaacccuuc ugaaggugua cccgaaucuc gcgcccgagu uuguuuaugg cgaauacccu     3060

aaauuccaaa ccuuuaagga gaacaaggcu acagccaaag ccauuaucua uacgaaucug     3120

cuccgcuucu uuaccgagga cgaacccaga uucacaaagg acggagagau ccuguggagc     3180

aacucuuacc uuaaaaccau uaagaaagaa cugaauuauc accagaugaa caucgugaag     3240

aaggucgagg ugcagaaagg cggguuuucc aaggaaagca uuaaaccuaa gggacccucc     3300

aauaaacuca ucccgguuaa gaacggccug gauccucaga aauacggggg auucgacucu     3360

cccauugugg cuuacacagu ccuguucacc caugagaagg gcaagaaacc ucuuaucaag     3420

caagagaucc uggggauuac gaucauggaa aagacacggu uugagcagaa ccccauucuc     3480

uuccuggaag agaagggcuu ucucagaccu cgcgugcuga ugaaacuucc caaguauacc     3540

cuguaccagu ucccggaagg acggagacgc cuccuggcca gcgcgaagga ggcucagaaa     3600

ggcaaucaaa uggugcugcc ugagcaccuu cugacccucc uguaucaugc caagcagugu     3660

cuccugccca accaguccga aucucuugcc uacgucgagc agcaccaacc cgaauuucag     3720

gagauccugg aacggguugu ggauuucgcu gaggugcaua cacuggccaa aagcaagguc     3780

cagcaaauug ugaaacucuu ugaggcuaau cagaccgccg acguuaagga aaucgcggcc     3840

uccuucaucc agcugaugca guuuaacgcu augggggccc cuucuacauu caaguuuuuc     3900

caaaaagaua uugagagagc ucgcuauacg agcaucaagg aaauuuuuga cgccaccauc     3960

auuuaccagu ccacaaccgg acuuuaugag acacggagaa aaguggucga uuga           4014


<210>  4132
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4132
augaagaaag acuacgugau cggccuggau auugggacca acagcgucgg augggccguu       60

augacagagg acuaucagcu cgugaagaaa aagaugccca ucuacggcaa uacggagaaa      120

aagaagauua aaaagaacuu cuggggagug agacuguuug aggaagggca caccgcugag      180

gaucggcgcc uuaaaagaac agcccggcgc agaaucuccc ggcgcagaaa ucggcugcgc      240

uaucuccaag cguucuuuga agaggcuaug accgaccugg augagaacuu cuuugccaga      300

cuucaggaau cuuuccuggu cccugaggac aagaaauggc aucggcaccc gauuuuugcc      360

aagcucgaag augagguggc uuaccaugag acauauccca ccaucuacca ucugcgcaag      420

aaacuggccg acagcuccga acaggcugau cucagacuga ucuaucuugc ccuggcgcac      480

auuguuaagu accggggcca cuuccucauc gaggggaaac ugucuacaga aaauauuagc      540

gugaaggagc aguuucaaca guucaugauc auuuacaacc agaccuuugu caauggagaa      600

ucccgccuug ugucugcccc ucugcccgag agcguucuca ucgaggaaga acugacggaa      660

aaagccucca gaaccaagaa aagcgagaag gugcugcagc aauucccuca ggaaaaggcu      720

aacggccucu uuggccaguu ccugaaacuu auggucggga auaaggcuga cuucaaaaag      780

guguuuggac uggaggagga agccaaaauu acauaugcgu cugaguccua cgaagaggau      840

cucgaaggca uccuggccaa ggugggggac gaguauagcg augucuuccu ugcugccaag      900

aacguuuacg acgcugugga gcugucuacc auccucgccg acuccgauaa aaagagccac      960

gccaaacugu cuuccagcau gauugugcgg uuuacagaac aucaggagga ccugaagaaa     1020

uucaagagau uuauccgcga aaauugcccc gaugaguaug acaaccucuu caagaaugag     1080

caaaaagaug gauacgcugg cuauauugcg cacgccggga aggucuccca gcugaaauuu     1140

uaccaguacg ugaagaaaau cauucaagac aucgcuggag ccgaauauuu ccuugagaag     1200

aucgcccagg aaaacuuucu gcggaagcag agaaccuucg auaauggcgu uauuccucau     1260

cagauccacc ucgcugagcu gcaagccauu auccaccgcc aggcggcuua cuauccguuu     1320

cuuaaagaaa accaggagaa gauugagcag cuggugacgu uccggauccc cuacuauguc     1380

ggcccucucu cuaaagggga cgccagcaca uuugccuggc ugaagagaca auccgaggaa     1440

cccauccgcc cuuggaaccu gcaggaaacc guggaucucg accagagcgc uacagccuuc     1500

auugagcgga ugaccaauuu cgauaccuac cugcccucug aaaaaguucu uccgaagcau     1560

ucccugcucu augagaaauu uaugguguuc aacgagcuga caaagaucag cuacacggac     1620

gacagaggaa uuaaggcuaa uuuuucuggc aaagaaaagg agaaaaucuu cgauuaccug     1680

uuuaagaccc gccggaaagu caagaagaaa gacauuaucc aguucuauag aaacgaauac     1740

aauacagaga uugugacccu uagcgggcuc gaagaggauc aauuuaacgc cuccuucucu     1800

acauaucagg accugcugaa guguggacuc acccgcgcgg agcuggauca cccugacaau     1860

gccgaaaaac uugaggauau caucaagauu cugacgaucu uugaagaccg gcagcggauu     1920

agaacacagc ucagcaccuu caaaggccaa uuuuccgcug agguccugaa gaagcuggag     1980

cgcaaacauu acaccgggug gggaagacuu agcaagaaac ucaucaacgg cauuuaugau     2040

aaggaaucug gcaaaacaau ccugggguac cugaucaagg acgauggagu guccaagcac     2100

uauaaucgga acuucaugca gcucauuaau gacagccagc ugucuuuuaa aaacgccauc     2160

caaaaggcuc aguccagcga gcaugaagag acccuuuccg aaacaguuaa ugagcuggcc     2220

ggcucucccg cgauuaaaaa ggggaucuac cagagccuca aaauugugga ugagcugguc     2280

gccaucaugg gauacgcucc uaagagaauc gugguugaaa uggcccgcga gaaccagacg     2340

accuccacag gcaaacggag aucuauucaa cgccugaaga ucguggaaaa ggcuauggcc     2400

gagauuggga gcaaucuucu caaagaacag cccaccacaa acgagcagcu gagagacacc     2460

cggcuguucc ucuauuacau gcagaacggc aaggacaugu auacgggaga ugagcugucc     2520

cuucaccgcc ugagccacua cgacaucgau cauauuaucc cccaaucuuu caugaaagac     2580

gauucccucg acaaucuggu ccuggugggc agcaccgaaa acagagggaa gucugaugac     2640

gugccuucca aagagguugu caaggauaug aaggccuauu gggaaaaacu uuacgcugcg     2700

ggacucauca gccagcggaa guuucagcgc cugacaaaag gcgagcaagg gggacugacc     2760

cucgaagaca aggcccacuu cauucagaga cagcuggugg agacacggca gaucaccaaa     2820

aauguggcug gcauucuuga ucaacgcuau aacgccaauu cuaaggagaa gaaaguccag     2880

aucauuacgc ugaaggccag ccucacaucc caguuuagaa gcaucuucgg gcuguacaaa     2940

gugcgggaag uuaacgacua ccaucacggc caggaugcuu aucugaauug cguggucgcc     3000

accacacuuc ugaaggugua cccgaaccuc gcgcccgagu uuguuuaugg agaauacccu     3060

aaauuccaaa ccuuuaagga gaauaaggcu accgccaaag ccauuaucua uacaaaccug     3120

cuccgcuucu uuacggagga cgaacccaga uucaccaagg acggcgagau ccuguggucu     3180

aauuccuacc uuaaaacaau uaagaaagaa cugaacuauc accagaugaa uaucgugaag     3240

aaggucgagg ugcagaaagg gggauuuagc aaggaaucca uuaaaccuaa gggccccucu     3300

aacaaacuca ucccgguuaa gaaugggcug gauccucaga aauacggagg cuucgacagc     3360

cccauugugg cuuacaccgu ccuguucaca caugagaagg ggaagaaacc ucuuaucaag     3420

caagagaucc ugggcauuac caucauggag aaaacgcggu uugaacagaa ccccauucuc     3480

uuccuggaag agaagggauu ucucagaccu cgcgugcuga ugaaacuucc caaguauaca     3540

cuguacgagu ucccggaagg ccggagacgc cuccuggccu ccgcgaagga ggcucagaaa     3600

gggaaccaga uggugcugcc ugaacaccuu cugacccucc uguaucaugc caagcaaugu     3660

cuccugccca aucagucuga gagccuugcc uacgucgaac agcaccagcc cgaguuucaa     3720

gaaauccugg agcggguugu ggauuucgcu gaggugcaua cccuggccaa auccaagguc     3780

cagcagauug ugaaacucuu ugaagcuaac caaacagccg acguuaagga gaucgcggcc     3840

ucuuucaucc agcugaugca guuuaaugcu augggagccc cuagcaccuu caaguuuuuc     3900

cagaaagaua uugaaagagc ucgcuauaca uccaucaagg agauuuuuga cgccacgauc     3960

auuuaccaaa gcaccacagg ccuuuaugag acccggagaa aaguggucga uuga           4014


<210>  4133
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4133
augcaggacu acuaucuggg ccucgauauc gggaccaaca gcgugggaug ggccgucaca       60

gacgagaauu acaaccugcu uagaaagaaa ggcaaggauc uguggggagu uagacucuuc      120

aaagaagcuc aacccgccac ggagcgcaga gggcaccggu ccucucgcag acggaccauu      180

cgcaagagcc ugagaaaaca gcuucugcgg gaacucuuug aggaagagau caaugcgauu      240

gacaaggauu ucuuucugcg ccuggacgag uccaaguauu ggcucgaaga uaaaaagauc      300

gagggcaaau acucucuguu caacgacgaa aauuuuacag auaaggacua uuacgagaaa      360

uucccuacca uuuuucaucu uagaaaggcu cugaucgaga gcgaugacaa ggaagauauc      420

cggcucuauu uccuggccau uaaccagaug augaaacgca gagggcacuu ucuuaucgac      480

ggaaagcugu ccaaugugga ggacauuaca ccguucaaag aaugcaucca gaaccucauu      540

aaugaggaac uggauaccga gaucgagaca gaauuuuacg acaaggccau uaaaaucgug      600

ucugauuaca gccugaagaa aaccgagaag aagaaccuca ucaaagaccu ggucaaggaa      660

acggauucca uugaggacag caaaaagauc auuaauaucu ucaaccuucu gauuucuggc      720

aaauccaagg ugaauggcau cuuugaugac gaagagaucc ucgagaagau uaaagaagau      780

aacaaggagg acuucuaucu gaccagcgaa aaauacgagg aaaaucuuga guauuucucu      840

caacugcucg auuaccgguu ugagcugauc uccagccuga agucuauuua ugacuacauc      900

auucuccagg aaguucugaa agggaagaag uaucuuuccu acgcucaggu ggagcgcuac      960

aaaaaccaua aggaagaccu gagaaaacuc aagcagcuga ucaaaaagua ugauaagaac     1020

ggaaaaaucu acuaucaaau uuuccgggac gagaauuuua agaaaggcua cgucgccuau     1080

cuuggguacu auaagaaaaa caagaaaaau gugcccguua agaaguguga uuacgacaca     1140

uucagcaaaa acaucaaguc cacccuggau aaaauuugcg acaaggagga uccucucuac     1200

aucgaacuga uugacaaaau cgagaaggga ggcuuucugc ucaagcagau ugugucuaca     1260

aauagcguca ucccccacca ggugcaugaa cuggagcuug auaaaauccu ggaaaaccuc     1320

gagaagaauu auccuuccuu caaagagauu agcgacgggg ugucuaagau cgauaaaauu     1380

aagaugaucu uuaacuuccg gauucccuac uaugucggac cucugaauga cuaccacaag     1440

gauaaaguua acuccaauac cuggaucgug aagaacgaag gcuuugagaa ugaaaacauc     1500

cgcccgugga auuucagcaa aauuguggac gagcacgcuu gugaagagga guuuaucaag     1560

cggaugauua acgaaugcuc uuauaucccc ggcgagaaag uccugccuaa gagcucccuu     1620

cucuacucug aauauauggu gcugaacgag cugaauaacc ucagaguuaa uggggacaag     1680

cugaacccca aaauuaagaa agaaaucgug gagaauaucu acaagaaaga gcgcaagguc     1740

acgcuuaaaa agauucggaa cuuccugauc auuaauaacu acguggauag caagguugaa     1800

uuuuccggac ucagcgacgg caucaaagcc aauaugucuu ccuauauuga uuucgcggag     1860

auccuggacg auaacuucga caauaacaug guggaagauc ugaucgagca gauuacaauc     1920

caugucggga augugaaacu ucucaccgag aaaauuagca acaaguaccc uaaucuggaa     1980

aagaagcaaa ucaacaagau ugucaaccug aaauuuaagg acugggccag auucucucgc     2040

aaacuccugg ugggaaucga gggcacagaa aaggagaccg gggaaauuaa uaccaucauc     2100

ggauuucuuc gggagacaaa cgagaauuuc auggaacuga uguccaacag auauacguuu     2160

agcgaggaaa uuuccgagau caaaaagaag uacauuccca auaaacucuc uaagcagauc     2220

guugaugacc uguaugugag cccggcuguc aaaaagauga uuuggcaagu gcugaacauc     2280

aaugaugaac uuaucaaagu ucucggcaag cagccuaaga aaauuuucau cgagauggcc     2340

cgcggcaagg aggaaaaacc cgaggugaag gacucccgga aaaacaagcu gauugaacug     2400

uacaagaauc ucgggaaaga gggaaaggaa uggcugaaca gaaucgagga uaaaaccgag     2460

gcugacuuuc gcaaucgggc ccuuuaucug uacuacacac agaugggcag auguauguau     2520

accggggaaa uuaucgacau cgagcggcuc uacgauaagg aacuguauga ccuggaucac     2580

auuaucccuc gcaaaaugaa gaaagacgau ucuauucaua agaaccuugu ccucgugaca     2640

agaaauagca accaguacga gaagaaagac aauuaucccg ugcccgaggu uaucaagucc     2700

cgggaaaacg ucagacaccu gugguacgcg cugaaaaaga aaggacucau uaccgaggaa     2760

aaguauaacc gccugacgcg gaucaauccu cuuagcgaua aggagcuguc ugaguucauu     2820

gaaagacagc ucguggagac acgccaaacc accaaagugg ucaaggaccu guuugaaaac     2880

uacuacacag auuccaaaau cgugaccguu aaggccggcc uggugagcga gcuuagacgg     2940

gacuucgaag uccugaaaug ccgcgagauc aaugaucuca accacgcuca ugacgccuuu     3000

cugaauauua ucguggggga cguuuggucu aagaaguuca cauccaaccc guauaauuuu     3060

gugaaaaaga acagagaaaa auacacgcuc aauaacauuu ucaccaagaa ucagaaagau     3120

cugcggggca acgagaucug gaauugugcu gagggaaaga agcgcauuau caaaacacuu     3180

aacaagccca gcguccuguu cucuguggaa aauuuugagc agaccggcca acucuucgac     3240

cagaacauca uugggaaaca ggauuauaag gaaaagacaa gcuacguucc uaucaaggga     3300

gacgagagac ugcaggauau uuccaaauau ggcggguacc ggggaaucag cggcgccuac     3360

uuuuucauug uggaacacac cgagaagaau aagcgcguca aaacgaucga gucucugccc     3420

aucuauauga aguccuacgu ggaagagaac gggcuucugg acuuuugcac caacgaaaug     3480

aaacucgugg auccuaagau uaucgucaaa aagcugaauu auaagagcga gauuaucguu     3540

gacggcuuca aauaccucau uuccggaaag acagagaaca gacugucucu ugaaaccaau     3600

gaguaucugu acuggaacca uacacagacc acggauauca aaaagauucu caauaucaac     3660

gagaaaaucg aacuggaaaa guauaauggc gacauugaga gcgagcggga aaacaucauu     3720

aaguacaucc aaauuauccu ggagaaauuu aaacuuaaac ccuucaucaa gcgcagaaau     3780

cugccgaaca uugaaaucaa uuuugccgau uaugagacag aaaaacuccu gcagcuccug     3840

gugaaccuua uuccucugac cacaaaguac gugucccccg ucgaccugac cgugaucucu     3900

gggagcaagu acgcuggaaa aguucucauu ccuaagaaaa ucuccaaucu gaacgaggug     3960

aucuucauuu cuaagagcau caccggcauu uaugaguccc ggagacgcau cauuuga        4017


<210>  4134
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4134
augaagcagg aguacuuccu gggccucgac auggggaccg gaagccuggg cugggccgug       60

acagauucca cguaucaagu caugagaaaa cacgggaagg cucuuugggg aacccggcug      120

uuugaaucug ccagcacagc ggaggaacgc agaauguucc ggaccgcucg cagacggcuc      180

gaccgcagaa acuggcggau ccagguucug caggagauuu ucuccgaaga gaucucuaaa      240

guggaucccg gcuucuuucu ucgcaugaag gagagcaaau acuauccuga agacaagaga      300

gaugccgagg ggaauugccc ggaacugccc uacgcccucu ucguggacga uaacuauaca      360

gacaagaauu accauaaaga uuauccuacc auuuaccauc ugcggaagau gcugauggag      420

acaaccgaga uccccgacau ucgccucguc uaccuggugc uucaccacau gaugaaacau      480

agaggacacu uucugcucuc cggcgauauc ucucagauca aggaauuuaa aagcacguuu      540

gagcaacuga uucagaacau ccaggacgaa gaacuugaau ggcacauuuc ccuggaugac      600

gcugccaucc aguucguuga gcaugugcuc aaggaccgga aucugacccg cagcacaaag      660

aaaucuagac ugauuaagca acucaacgcu aaauccgccu gugagaaggc gauccugaau      720

cuucugagcg gcgggaccgu caaacucucu gauauuuuua acaauaagga acuggacgag      780

uccgaacggc cuaaagugag cuucgccgau ucuggauaug acgauuacau cggcaucguu      840

gaggcugaac uugccgagca guauuacauu aucgcuuccg ccaaggcggu guaugacugg      900

agcguccugg uggagauucu cgggaacucc gugucuauca gcgaagccaa gauuaaaguc      960

uaccagaagc accaggcuga ucugaaaaca cugaagaaaa ucguuagaca auauaugacc     1020

aaggaggacu acaagcgcgu guuuguggau acggaagaga aacucaauaa cuacuccgcc     1080

uauaucggaa ugacaaagaa aaauggcaag aaagucgacc uuaagagcaa gcagugcacc     1140

caggcugauu ucuacgacuu ccugaaaaag aacgugauua aaguuaucga ccauaaggaa     1200

auuacacaag agaucgaguc ugaaauugag aaagaaaauu uucugcccaa acaggugacc     1260

aaggauaacg gggucauccc uuaucaggug cacgacuacg agcucaaaaa gauccuggau     1320

aaccuuggaa cccggaugcc guucauuaaa gagaaugccg aaaagaucca gcaacuguuu     1380

gaguuccgca uucccuauua cguuggcccu cucaacagag uggacgaugg caaagacggg     1440

aaguuuacau gguccguccg gaaaagcgau gccagaaucu aucccuggaa uuucacggaa     1500

gugauugacg ucgaggcuuc ugcggaaaag uuuauccgcc ggaugaccaa caaguguaca     1560

uaccuggugg gagaggaugu ucugccuaaa gacagccucg uguacuccaa guucaugguc     1620

cugaaugagc uuaacaaucu gagacucaac ggcgaaaaga uuucugugga gcugaagcag     1680

cgcaucuaug aggagcuguu uugcaaauac cggaagguua ccagaaagaa acuugaacgc     1740

uaucucguga ucgaggggau ugccaagaaa ggagucgaga ucacaggcau ugauggggac     1800

uucaaggcua gccugaccgc cuaccacgau uuuaaagaac ggcugacgga cgugcagcuc     1860

ucccagagag ccaaggaggc uaucgugcug aauguugucc uuuucggaga cgauaagaaa     1920

cugcucaagc aacggcugag caaaauguau cccaaccuga caaccggcca gcuuaagggc     1980

auuuguucuc ucuccuacca ggggugggga cgccugagca aaaccuuucu ggaagagauc     2040

acagugccgg ccccuggcac cggggaagug uggaauauca ugacagcgcu cuggcagacg     2100

aacgacaauc ugaugcaacu ucugucuaga aacuauggau ucaccaauga ggucgaggag     2160

uucaacacac ucaagaagga aaccgaucug uccuacaaaa caguggacga acuguacguu     2220

agccccgcug ugaagcggca gauuuggcag acccuuaaag ucgugaagga gauccagaaa     2280

guuaugggca acgccccuaa gagaguguuu gucgagaugg cccgcgaaaa acaagagggg     2340

aagcgguccg auucuagaaa gaaacagcuc guggaacugu aucgcgcuug caagaaugag     2400

gaaagagacu ggauuacgga gcugaacgcc cagagcgauc aacagcuccg guccgacaaa     2460

cuguuccuuu acuauaucca gaagggccgc uguauguacu cuggagagac cauucagcug     2520

gaugaacucu gggacaauac aaaauaugau aucgaccaua ucuaccccca aagcaagacc     2580

auggaugacu cccugaacaa uagaguucug gugaagaaaa acuauaaugc uauuaagagc     2640

gacacauacc cccuuucucu cgauauccag aaaaagauga uguccuuuug gaaaaugcug     2700

cagcagcaag gcuucauuac caaggagaag uacguccggc uggugcgcag cgacgaacuc     2760

ucugccgaug agcuggcggg guuuaucgaa agacagauug uggagacgcg gcaguccaca     2820

aaagccgucg cuaccauccu uaaggaggcc cugccugaca cagaaaucgu uuaugugaaa     2880

gcuggaaacg ugagcaauuu ccgccagacc uacgagcucc ugaaggucag agaaaugaac     2940

gaucugcacc augccaaaga cgcguaucuu aauauugugg uuggcaacgc cuacuuugug     3000

aaguucacca agaaugcugc cugguuuauc cggaacaauc cggggcgcuc uuauaaccug     3060

aaaagaaugu ucgaguuuga uauugaacgg agcggagaga ucgcuuggaa ggccggcaac     3120

aaagggucca uugucacagu gaagaaaguu augcaaaaga auaacauccu cgugacgaga     3180

aaggccuacg aggucaaagg cggacuguuc gaccagcaga uuaugaagaa aggcaagggg     3240

caagugccca ucaaaggaaa ugaugaacgc cucgcugaca ucgagaagua uggcggguac     3300

aacaaagcgg ccggaaccua cuuuaugcug guuaagagcc uugauaagaa aggcaaggaa     3360

auucggacaa ucgaguucgu gccucuguau cucaaaaauc agauugagau caaccacgaa     3420

ucugcuauuc aguaccuggc ccaggagaga gggcuuaauu cccccgaaau ccugcugagc     3480

aagaucaaaa uugacacccu cuucaagguc gacggcuuua agauguggcu guccggacgc     3540

acaggcaacc aacucaucuu caaaggggcc aaucagcuga uucuuucuca ucaggaggcu     3600

gccauccuga agggaguggu gaaauauguc aaccggaaga augaaaacaa agaugcgaag     3660

cucagcgaga gagacggcau gaccgaggaa aagcugcugc agcuuuacga uacguuucug     3720

gacaaacucu ccaauacagu uuauucuauu cgccugagcg cucaaaucaa gacccucacc     3780

gagaaacggg ccaaguucau cgggcugucc aacgaagauc agugcauugu gcuuaaugag     3840

auccugcaca uguuucagug ucagucugga agcgccaacc ugaaacucau uggcgggccu     3900

ggcuccgcug gaauccuggu gaugaacaau aacauuacag ccugcaagca aaucagcguc     3960

auuaaucagu cucccaccgg caucuacgaa aaggagaucg accuuauuaa acuguga        4017


<210>  4135
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4135
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

guuacggaug gcuauaaagu gccugcuaag aaaaugaagg ugcucgggaa uaccgacaaa      120

ucucacauca agaagaaccu gcuuggagcc cuucucuucg auagcggcaa uacagcgaaa      180

gacagacggc ugaagcgcac cgcuagacgg cgcuacacaa gacggcgcaa ccggauucuu      240

uaucugcagg agaucuuugc cgaagagaug gccaaagcug augaauccuu cuuucaaaga      300

cucaaugagu cuuuccugac caacgacgau aaggaauuug acagccaucc gauuuucggg      360

aauaaagccg aggaggaagc ucaccaucac aaguuuccca caaucuucca ucugcgcaag      420

caccucgccg auuccaccga gaaaucugac cugagacuua ucuaccuggc gcucgcccac      480

augauuaagu uucggggaca uuuccugauc gaaggccagc uuaaagcuga gaacacgaau      540

guccagaccc uguuugauga cuucguggag guuuaugaua agacagugga agagagccac      600

cucuccgaaa uuagcgucuc uuccauccug accgagaaaa uuagcaaguc ucgcagacug      660

gaaaaccuca ucaaauacua uccuacagag aagaagaaua cccuguuugg caaccuuauu      720

gcccuguccc ucgggcugca gcccaauuuc aaaacgaacu ucaagcugag cgaggacgcu      780

aaacugcaau uuucuaagga cacauacgaa gaggaucucg gagaacugcu gggcaaaauc      840

ggggacaauu acgccgaucu cuucaucucc gcgaagaacc uguaugacgc cauucuucug      900

agcggaaucc ucaccauuga ugacaacaca accaaggcuc cucuguccgc cucuaugauc      960

aaacgguacg aggaacauca ggaggaucuu gcucagcuga agaaauuuau ucgccagaau     1020

cuccccgacc aauauagcga gguguucucc gauaagacca aagacggcua cgccggguau     1080

aucgauggaa agacaaacca ggaagccuuu uacaaguaua ucaaaaauau gcugagcaag     1140

acggagggcg cugacuacuu ccuggacaaa auugauagag aagacuuucu ccggaagcag     1200

agaaccuucg auaacggcuc uguuccucac cagauccauc ugcaagagau gcacgcgauu     1260

cuucgccggc agggggaaua cuauccguuu cugaaagaga aucaggacaa gaucgagaag     1320

auucucacau ucagaauccc cuacuaugug ggaccucugg cccgcaaagg cucccgguuu     1380

gcuugggccg aauacaaggc cgauaaaaag gucacccccu ggaacuucga cgauauccug     1440

gacaaagaaa agagcgcuga ggaguuuauu acaagaauga cccuuaauga ucucuaucug     1500

ccugaggaaa aagugcugcc caagcacucu cucguguacg agacguucaa cgucuauaau     1560

gaacugacaa agguuaaaua cgugaacgag caagggaagg ccaucuucuu ugacgcgaau     1620

augaaacagg aaauuuucga ucauguguuu aaggagaacc gcaaagucac caaggacaaa     1680

cuucugaauu accucaacaa agaguucgaa gaguuucgga ucgugaaucu gaccggacug     1740

gauaaggaaa acaaggcuuu caacagcucc cuuggcacau aucacgaccu cagaaagauu     1800

cuggacaaau cuuuucugga ugacaagguu aaugagaaga ucauugaaga uaucauccag     1860

acccucacac uguucgagga ccgcgagaug auucggcaga gacuucaaaa auacagcgau     1920

aucuuuacga cccagcagcu gaagaaacuc gaacgccggc auuauacagg guggggaaga     1980

cuguccgcca agcugauuaa cggcauccgg gacaaacaga gcaauaagac cauucuuggc     2040

uaccucaucg augacgggua uucuaaccgc aauuucaugc aacugaucaa cgaugacucc     2100

cugccguuua aggaggaaau ugccagagcu caggugaucg gagagacaga ugaccucaau     2160

cagcugguca gcgauauugc cggcucuccu gcuaucaaaa aggggauucu ucagucccug     2220

aaaaucgugg acgaacucgu uaaggugaug ggacacaacc ccgccaauau cgucauugag     2280

auggcgcggg agaaccaaac cacggccaaa ggcagacgca gcucccagca gcgguacaag     2340

agacuggaag aggcuaucaa aaaucuggac cacgaucuua accauaagau ucucaaggaa     2400

cacccuaccg acaaucaggc ccugcaaaac gaucgccugu uccucuauua ccugcagaau     2460

gggagagaca uguacacaga ggauccccuu gacaucaacc ggcugucuga uuaugacauu     2520

gaucauauca ucccccagag cuuuauuaaa gacgauucca ucgacaacaa ggugcucguc     2580

ucuagcgcua aaaaucgcgg caaguccgac aacgugccua gcgaggaugu ugugaauaga     2640

augcggccgu ucuggaacaa acugcugucu ugcggacuua uuucccaacg caaguacagc     2700

aaucugacca agaaagaacu caagcccgac gauaaagccg gcuucaucaa gagacagcug     2760

gucgagacac ggcagauuac caaacacgug gcgcagaucc ucgacgcccg cuuuaacacg     2820

aagagagaug aaaauaagaa aguuauucgg gacgugaaga ucaucacacu gaaaucuaac     2880

cuugucuccc aauuccgcaa ggauuuuaaa uucuauaagg ugagagagau uaaugacuac     2940

caccaugcuc acgaugccua ucugaacgcu gugaucggga aggcccuccu ggacguuuac     3000

ccucagcugg aacccgaguu ugucuaugga gaguacccuc auuuccacgg cuacaaagaa     3060

aauaaggcca ccgcuaaaaa guuuuucuau agcaacauua ugaacuuuuu caaaaaggau     3120

gacauccgga cagaugagaa uggggaaauu guguggaaga aagacgagca uaucucuaau     3180

aucaagagag ugcuuagcua cccccagguc aacauuguga aaaagguuga aauccagacc     3240

gugggacaaa acggcgggcu guuugacgau aauccgaaau ccccucucga ggucaccccc     3300

agcaagcugg ugccucucaa aaaggagcug aaccccaaga aauauggcgg auaccagaag     3360

ccuacaacgg cguaucccgu ucuucugauu accgacacaa aacagcucau cccgauuucu     3420

gugaugaaua agaaacaguu cgaacaaaac ccugucaagu uccugcgcga ucggggcuac     3480

caacaggugg ggaagaauga cuuuaucaaa cugcccaagu auacccuugu ugauaucgga     3540

gacggcauua aaagacugug ggccuccagc aaggagaucc acaaagggaa ccagcucgug     3600

gucuccaaga agucucagau ucugcucuac cacgcucauc accuggauag cgaccuuucc     3660

aaugauuacc ugcagaacca uaaucagcaa uucgacgugc uguuuaacga aaucauuucu     3720

uucagcaaaa aguguaaacu cggaaaggag cacauccaga aaauugaaaa uguguauucc     3780

aacaagaaaa auucugccag caucgaggag cuggccgaau ccuuuaucaa acuucugggc     3840

uucacacagc ucggggcuac cagccccuuu aacuuccugg gcgucaagcu caaucagaaa     3900

caauacaagg gaaagaagga uuauauucug ccuugcacgg agggcacacu uauccgccag     3960

ucuauuaccg ggcuguacga aacccggguu gaccugucca aaaucggaga ggauuga        4017


<210>  4136
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4136
augagagacu acuauaucgg ccuggaucuc gggaccggaa gccugggcug ggccgugaca       60

gaccgggagu acgaaauuau gcgcgcucac gggaaggccc uuuggggagu cagacuguuc      120

gauuccgcga acacggcuga ggaacggcgc ggcuuuagaa ccgcccggcg cagacucgac      180

cggcgcaauu ggagaaucga gcugcuucag gaacuguucg gggaggauau uggaaaaguu      240

gacucuggcu uuuuccuccg gaugaaggag agcaaauaua ugcccgaaga uaagcgcgac      300

gugaacggca auugcccuaa acugccguac gcccuguuug uggaggaugg guauacagac      360

aaggauuacc auagacaauu ccccaccauc uaucaccucc ggaaguggcu gauggaaaca      420

gaggagaccc cugacauucg ccuugucuac cuggcucucc aucacaugau gaaacauaga      480

ggacacuuuc uguucuccgg caacaucgaa aagaucaaag aguuucagga aacauuccgg      540

caguacauug ggaagaucag agaggaagaa cuugauuuuc accuguguau ugagggagaa      600

gaacuccgcg aaaccgagaa uauccugaaa gacaagaacc ugacgcgguc ugccaagaaa      660

accagacuca uuaagcugcu uggcgcucau acagccugcg aaaaagcggc ccugaaucuc      720

guggcugggg gaaccguuaa gcugagcgac aucuucggca acuccgagcu ugaugccugu      780

gagaaaccca agcugucuuu ugcugacgcc ggcuaugaug acuacgcggg gaugauugaa      840

gaugagcucg gagaacagca cgugaucauc gagacagcca aagcugucua ugacuggagc      900

gugcuggccg auauucuggg cgacuaccgc ugcaucuccg aagcuaaggc cgccguuuau      960

gagaagcauc aaaaagaucu ccggcaccug aaggagcuug ugaaagaaaa ucuggggaga     1020

gacgucuaca aggagguguu cgugaaaacc aacgaaaagc ucccuaauua uagcgcuuac     1080

auuggaauga cgaagaaaaa cggcgucaag ucugagaugg aggggaaacg cugugaucgg     1140

aaggcguucu acgacuaucu gaaaaagaca guugugaaug ccauccccga cgaauccaag     1200

accgaguacc uuagaaaaga aauggagaca gaaaccuuuc ugccucgcca ggugaccaag     1260

gauaacggag ucauuccgca ccaggugcau cuccaggagc uggacgcuau ccuggagaau     1320

cucagcggcc ggauccccgc ccugaaagaa aacggcucua agauuagaga uaucuucaca     1380

uuucgcauuc cuuauuacgu ugggccccuu aacggaaucg ugaaaggcgg ggagcggacg     1440

aauuggguca gacggaagaa ggccggacgc auuugcccuu ggaacuucga cgaaauggug     1500

gauaccggcg cuuccgccga ggaauuuauc agacggauga caagcaaaug uaccuaucug     1560

auccacgagg acguucuccc caaaaauucu augcuguacu ccaaauucau ggugcugaac     1620

gagcuuaaua acgucagacu caauggggaa ccgauuagcg uggagcugaa gcaaaagaca     1680

uaugaagauc uguuucagcg ccaucggaag gucaccagac gcagacucac ggacuacauc     1740

cggcgcgagg gcauugcggg aagagaugcu gacaucacag gcauugaugg ggacuucaaa     1800

ggaucccuga ccgccuacca cgauuuuaag gaaaaacuua ccggcugcga gcugucucag     1860

gccgacaaag agaacaucau ucucaauauc acacuguucg gggaagacaa ggcucugcuu     1920

aaaaagcggc ucggagcccu guauccugcu cugaccgagc cccagaaaaa ggccaucugu     1980

gcgcucagcu acaagggcug ggggcgccug ucccaaagac uucuggaagg cauuacagcc     2040

ccugcucccg agacgggaga gaucuggacc gugauucggg ccauguggga aacaaacgau     2100

aaucucaugc agguucugag cgagaaauau ugcuuugcug ccgcgaucga cgaagagaac     2160

gccggcgagg agcugaagga gauuaccuac aaaacagugg aacagaugaa ugucucuccc     2220

gcugugcgca gacaaaucug gcagucccuu cagguuauca aggagauuug uaaagugaug     2280

ggagggccuc cgaagcgggu cuucguggaa auggcccgcg agaagaugga aagcaaaaga     2340

accgagucuc ggaagaaaag acucaucgau cuguauaaga aaugccgcga ggaggagcgg     2400

gacuggauug aggaacuggg caacacggaa gagaccagac uccgcagcga uaagcuguac     2460

cuuuauuaca cacagaaggg gcggugcaug uacuccggag aggugaucga acuggaggaa     2520

cucugggaca auagaaaaua ugauauugac cauaucuacc cccaaucuaa gguuauggau     2580

gacagccugg auaaccgcgu ccuggugaaa aaggaguaua acgcugacaa aaccgaugag     2640

uacccuaucc gggccgacau uagaggcaag augcgcgccu uuuggcggau ccuuagagaa     2700

gagggguuca uuuccaaaga aaaguauaau cggcucacac gcggcaccgg auucgagccc     2760

agcgaacugg cuggcuuuau cgcgagacag cugguggaga cgcggcaggg gacaaaggcc     2820

gucgcuucug ugcucaaaca gguuuucccu gagaccgaca uuguguacgc caaggcccgc     2880

gucgcuuccc aauuuagaca ggaguucgau cugaucaaag ugcgggaaau gaacgaccuu     2940

caccacgcca aggaugcgua uguuaauauc guggucggaa acguguacua cacaaaauuu     3000

accagcaaug cugccuggua uguuaaggaa caucccggca gaucuuacaa ccugaagaaa     3060

auguucaccu ccgagcgcga cguggcccgg aauggggaaa cagcuuggag agccggaaac     3120

agcggcacga uugcgaccgu caagcgcgug auggggaaaa auaacauccu cgugacacgg     3180

agauccuaug aggucaaggg cggacuguuu gaucagcagc ugaugaaaaa gggcaagggg     3240

caaguuccga uuaaaggacg cgacgagcgg cuugcugaua ucgacaagua cggcggguau     3300

aauaaagccg ccggaaccua cuucaugcug gcugaaucug aggauaagaa aggcgccaag     3360

auuagaagcg uggaauaugu gccucucuac cuguguaacu gcaucgagaa ggacgaagag     3420

gcugccaaaa aguaccucca gaaagagcgc ggccugaaga aucccagagu ccuuauugcg     3480

aaaaucaaga ucgauacacu guuuaaggug gacggguucu auauguggcu cuccggacgg     3540

accggcaacc agcugauuuu uaaaggggcc aaucagcuga uccuuucuga accugacaug     3600

cgcauucuga agaaaguucu caaguacgug aacagaaaga aggagaacaa aaaugcuguc     3660

cugggagaac acgaucaacu ccccgagacg gaccugaucc ggcuuuauga uguguuccug     3720

gacaagauug agaacacagu uuaccaugug cgccucagcg cccagcaggg cacccugacc     3780

aagaauaaag auacauuuug ugaacugucc aacgaggaca agugcaucgu ccuuagcgaa     3840

auccugcaca uguuccaaug ucagucuggg uccgcuaauc ucaaacugau uaagggaccu     3900

ggcagcgccg gcauccucgu gcugaacaau auuaucucua aaugcaacca gguuuccauu     3960

auccaccaga gccccaccgg gaucuaugag caagaaauug aucuuaagaa gaucuga        4017


<210>  4137
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4137
augaacaagg ccuacacccu gggccucgac aucgggacaa auagcguggg augggcuguc       60

guuacggaug acuauagacu gauggccaaa aagaugcccg ugcacuccaa aauggagaag      120

aaaaagauua agaaaaacuu cuggggcgcg cggcuuuuug augaagggca gaccgcugag      180

gaacgcagaa auaagcgggc cacacgcaga cggcugcgca gacggaaaua ccgcauccuc      240

gagcugcaaa agauuuucuc ugaagagauc cuuaaaaagg acagccacuu uuucgccaga      300

cuggaugagu ccuuucucau uccugaagac aagcaguaug cucgguuccc gaucuuuccc      360

acccugcugg aggaaaaagc cuacuaucag aacuacccua caaucuauca ccuccgccag      420

aagcuggcug auucuaccga gaaagccgac auuagacuug uguaccuggc gcucgcccau      480

augaucaagu accggggaca cuuccuguuu gagggcgaac uugauacaga gaauaccagc      540

gucgaagaga cguucaaaga auuuauugac aucuauaacg agcaauucga ggaagggauu      600

aucuuuuaca aggauauucc ccugauccuc accgacaaac uguccaaguc uaagaaagug      660

gagaagaucc ugcaguauua cccuaaagaa aagacaaccg gaugccucgc ucaguuccug      720

aaacuuauug uuggcaauca gggcaacuuc aagcaagccu uucaucugga ugaggaagug      780

aagauccaga uuagcaaaga gacauaugag gaagaccucg agaagcugcu ucgcaaaucc      840

aaugaagaga ugaucgacgu cuuccuccag gugaagaaag uuuacgaugc uauucugcug      900

agcgacaucc ugucuaccaa gaugaaggau acgaaagcca agcucuccgc ggggaugauc      960

gaaagauauc agaaccacaa aaaggaccug gaggagcuua aacaauuugu gcgggcccau     1020

cugcaugaaa aggucacagu guucuuuaag gauagcucua aagacggaua cgcuggcuac     1080

auugauggga agaccacaca ggccgacuuc uauaaauuuc ucaagaaaga gcugaccgga     1140

gugcccggcu ccgaaccuau gcuugcuaag aucgaucagg agaauuuccu gcucaaacaa     1200

agaaccccga caaacggggu cauuccccac cagguucauc ugacggaguu uaaggccauc     1260

auugaccagc agaagcaaua cuauccuuuc cuggaaaaga gcaaggagaa aaugauccag     1320

cuccugaccu uucgcauccc cuacuaugug ggaccucuug cccaggauaa ggaaacaucu     1380

uccuucgcuu ggcuggagcg gaaaaccaca gaaaagauua agcccuggaa ugcgaaagac     1440

gugaucgacu acggcgccag cgcuaccaag uuuauucaga gaaugaucaa cuaugauacg     1500

uaccucccga cagagaaagu ccugccuaag uacuccaugc uguaucaaaa auacaccauu     1560

uucaaugagc uuaccaaggu ggccuauaag gacgaucgcg gcaucaaaca ccaguucucu     1620

agcgaagaga agcuccggau uuuucaggaa cuguucaaaa agcagagacg cguuacaaag     1680

aagaagcugc aacacuuucu cuccgccaac uacaacaucg aggacgcuga aauccugggg     1740

guggauaaag ucuucaauag cucuuaugcc accuaccaug acuuucuuga gcuggcgaag     1800

cccuauacag agaaaauuau cgaacuccug gagcagccug aaauggagga aauguucgag     1860

gauauuguga agaucauuac gaucuuugag gaccgggaaa ugguuagaac ccagcugaaa     1920

aaguaccagc gcauccuugg agaggaaauu uucaaaaagc ucgugaagaa aaaguacaca     1980

ggcugggggc ggcuguccaa aagacugauc aacggcauuc gcgaucaaaa gaccaauaaa     2040

acaauccucg acuaucugau uaacgaugac gauuuucccu acaaucggaa cagaaauuuc     2100

augcagcuua ucaacgacga ccaccugagc uuuaaggagg agaucgcuaa ggaacucacc     2160

cugucugaua aacagagccu gcuugagguc guggaagcca uucccggauc cccugccauc     2220

aagaaaggga uuuggcaaac gcucaagauc gucgaggaac ugauugcuau caucggauau     2280

aaaccgaaga auauugugau cgagauggcc cgggagaacc agaccacaac cggcggcaag     2340

aaucgcucua aacccagacu caagagccug gaagaggcuc ugaaaaacuu cgacucccag     2400

cuucugaagg aacggccugu ugauaaucag agccuccaaa aagacagacu guaccuguau     2460

uaccuucaga acgggaagga uauguauaca ggagagucuc ucgacauuga ucgccugucc     2520

gaauacgaca ucgaucauau uaucccccgg agcuuuauug uggaccacuc ucuggauaau     2580

aagguccucg uguccagcaa agagaacaga cugaagaaag acgauguucc ugacuccaag     2640

guggucaaac gcaugaaggc cuacugggag aaacuucuga gagcgaaccu caucucugaa     2700

cggaaauuca gcuaucugac caagcuggag cuuacggacg augacaaagc ccgcuucauc     2760

cagagacagc ugguggaaac acggcaaauu accaagcaug uggcugccau ccuccaccag     2820

uacuuuaauc agacacagga gcuggaaaaa gagaaggaua uucgcaucau uacccucaaa     2880

uccucucugg uuagccaauu cagacagguc uuuggcaucc acaaggugcg ggagaucaac     2940

caucaccauc acgcucacga cgccuaucuu aaugcggugg ucgcccuggc ucuccugaag     3000

aaauaccccc gccuggcccc ggaguucgug uauggguccu uugcuaaguu ccaucuuguu     3060

aaagaaaaca aggccaccgc caaaaaggaa uuuuacagca auauucugaa guucuuugag     3120

aaagaggaac aguucuguga ugagaacgga gaaaucuuuu gggacaagag aaaacacauu     3180

caacagauca agaaagugau uucuucccau caggucaaua ucgugaagaa gguugaggug     3240

cagacaggca gcuucuauaa agaaacgguc aacaccaagg agaaaccuga uaagcucauc     3300

aaacggaaga auaacuggga cgugacaaag uacgggggcu ucggaucucc cguuguggcu     3360

uacgcggucg uguuuaccua ugagaaaggc aagaaucaca aaaaggccaa agcuauugaa     3420

gggaucacaa uuauggagca agcccuguuc gaaaaggauc cuaucuccuu ucucauugag     3480

aaaggauaca gcaacgugaa ucaguucauc aagcugccca aguauacccu uuuugaacug     3540

gccaacggcc agcgcagaau gcucgcuucu caucaggagc ugcaaaaagc caauagcuuc     3600

auucugccug agaaacuugu cacgcugcuc uaccacgcga accacuauga cgaaaucgcu     3660

uacaaagauu ccuaugacua cguuaacgag cauuuuagca auuuccagga cauccuggau     3720

aaggugauua ucuuugccga aaaauacaca ucugcucccc agaagcucaa ccagauuauc     3780

gccaccuaug agaagaauca agaagccgac cggaaaauua uggcgcacuc cuucgugaac     3840

cugaugcagu uuaaugcucu uggggccccg gccgauuuca aguuuuucga caccacaauc     3900

accagaaaac gcuacacaag ccugacggag aucuggcagu ccaccauuau cuaucagucu     3960

gucacaggac uguacgagac ccggagacgc auggcugauc ucugggacgg cgugcaauga     4020


<210>  4138
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4138
auggagagau accacaucgg ccuggacauu gggaccagcu ccaucggaug ggccgugauu       60

ggcgaugacu ucaagaucaa aaagaaaaag gggaaaaacc ucauuggaac acggcuguuu      120

aaugaaggcg cuacggccgc ggagcgcaga ggguuccgga cccagcgcag acggcuuaac      180

cgcagaaagu ggcggcugaa gcuccuggaa gagaucuuug aucccuauau ggcugaaguc      240

gacgaguacu ucuuugcccg ccuuaaaucu agcaaucugu ccccuaagga uucuaacaaa      300

aaguaucucg gaagccugcu guucccggac aaauccgauu cuaauuuuua cgacaaguau      360

cccacaaucu accaucucag acgggaucug auggagaagg acgaaaaauu cgaucuucgc      420

gagauuuauc uggccaucca ccauauuguu aaguacagag gcaacuuucu cgaaaaagug      480

ccugcuaaga auuacaaaaa cagcggcgcc uccaucgggu uccugcuuga ggaggugaau      540

agccuguaua aggacauuau cggagaugaa ucuauugcua uccucaacuc cgagaaauuu      600

gaagacgucg agaagaucau ucuggacgaa gagacccgga aucuggauaa gcaaaagagc      660

gugggcaagc uccugguuga ggacaaaaag aaaaagaaca ucgugacagc cuucucuaag      720

gcgauuuuug gguacaaauu caauaucgaa gaucuucugc ucauugagaa cgacgaaaag      780

aauaaacuga ccuucaacga ugagaacauc gacgauaucu uuaaugaacu uucccacagc      840

cuggacgaua agcagaugga ccuccugaca aaaacccgcg agauuuauuu caaguuuaag      900

cugaacauga ucguccccac gggauacacc cucucugagu ccaugauuga aaaauaugag      960

augcauaagg cccaccugaa aauguacaag gaguucauca auacacuuaa caccaaagau     1020

agaaagauuc ugaagaaugc uuauagcgac uacaucaaca cagagaaagc caaggcugcc     1080

aaugcgcagg aaaacuuuua caaaaccgug aagaaaauca uuaaggacaa uaacuccgau     1140

acggccaaaa agaucauugu ucucaucgac gagggcaauu ucaugccuaa gcagcggaca     1200

ggggagaacg gagugauucc ccaccaacug caucagaucg aacuugauag aauuaucgag     1260

aaucaggcua aauauuaccc uuggcuggcc gaagcuaacc cggucgagaa gaaucgcaaa     1320

uuugccaagu auaaacucga cgagcuggug accuuccggg ugcccuacua ugucggcccu     1380

cugaucgaua agacagaauc uaacaagaac gagaaagaaa ccaaguuugc cuggaugguu     1440

agaaaagcua agggcaccau uacacccugg aauuucgaga accucgugga ccgcacggaa     1500

agcgcgaauc gguuuaucaa aagaaugacc uccaaggaua cauacauuau cggggaggac     1560

gugcugccug ccagcucucu ucuguaugag aaguucaaag uccucaacga acugaauaac     1620

auuaagguga augagaaaaa gcuggauauc gaacagaaac aagacaucua ccuuaaccuc     1680

uuuaccacag cuaagaaugu uaccaagaaa ucccuggcca cguaccugaa cugcagcgcc     1740

gauucuauua gcggacucuc cgacggcgag aaguucaauu cuagccuguc cagcuauauc     1800

gaucuuaaag cuauucuggg gaacaucgug gacgacugua auaagaacga agaucucgag     1860

aaaauuaucg aguacucuac agucuucgaa gacggaaaua ucuauaagga gaaacugucc     1920

gaaauuagcu ggcugaccga ugagcagauc gaaaaacuuu cuaacauuca cuuuaagggc     1980

ugggggcgcc ucuccaaaaa gcugcugacc cagaucacaa acgagaaugg agagcggauu     2040

aucgacgccc ucuggaacac cagcaauaac uucaugcaga ucauuaauga ugaauccauc     2100

caagcgaaac uggcugagau uaacggcaag uacgccggca aaucugaccu ugaagauauc     2160

cugagcgagg ccuauacauc uccccagaau aagaaggcua uuagacaggu gaugaaaguu     2220

guggaggaca ucgaaaaggc caugcaaugc gagccgacgu ccauuagcau cgaauuuacc     2280

cgcaaaaagc agaaaucccg gcucacaaac accagauaca agaagaucag cgaugucuau     2340

gagaaaauug cugacgaagu gaucucugag uacgagcugc gcaagcugca guccgaacuu     2400

gauagcaaug ccaacaauau gcgggacaga uacuaucucu acuucaugca gcuggggcgg     2460

gauauguaua caggagagaa aauuaacauc gacgaacugc aucaacgcua cgacauugau     2520

cacauccucc cucagucuuu uaucaaggac gauuccauua auaacagagu ccugacccgg     2580

aaagacguga auaacaagga aaagagcgau aaaacggcgg ccgaccuuua ugcuguugaa     2640

augucugauu ucuggagaaa gcugcgcaaa cagggccuca ucaccgagca gaaguacaaa     2700

aaccugcuga caaagagcga cuccauugau aaguaugcca aaaagggguu uaucaaacgg     2760

caacuugugg agaccagcca ggucgugaag cucguugcua auauucugca ggacaaauac     2820

ucugauacaa agaucaucga agugagagcc aaacugaacu ccaaucuccg cgaggaauac     2880

aagcugauua agaacagagc ggucaaugac uaucaccaug ccaucgacgg auaccuuacc     2940

acguucauug gccaguaucu guacaaaaca uaucccaagc uccggagcua cuuuguguau     3000

aacgauuuca aaaagcugga cuccaauuac cugaaacaca ugaagaaguu uaacuuccuu     3060

uggcaacugc aggaugacaa aaccaucgau auuuacgaca augugaacaa uaaguuuguu     3120

cucaacgucc cugagaugaa aaaguauauc cagaaaauuu acaauuauaa guacaugcug     3180

gugucuaagg aagugacaac caaaaacggg gcuuucuaug aucagaccaa guacaaugcc     3240

aaaacaguca accucauccc caucaagaaa aauaagccca cgaacauuua uggcggauac     3300

aagggcaaag ugagcuccua caugaugcug guuaagaucc gcaaaaagaa agaggugauu     3360

uauaaguucg ucggggugcc uagacuuugg accgacgagc uggaucggcu caaagacaca     3420

accgaacgca agauccugcu gaacgagauu gcuaaaaauu cucuuagcaa gacagaacaa     3480

gauuuugagg uuauccugga caagguguac uauggacagc ucaucauuga uggcaaccag     3540

aaauacaccc ugggguccuc ugaguauaag uacaaugcca ugcaacucca ucugagcaaa     3600

cacgcccuug aaguccuggc uaaggagaac gugaaggacg cggaaaucac ggacaaagau     3660

cucguuuccg uguaugagga aauucugagc gucgugaaua aguacuucga gcuguacgac     3720

aucaacaaau uuagacagaa acuuaaugag ggacuggaaa uuuuccggaa gcucccggug     3780

uauaacgucu acgagucuaa uaagaucaag cagguuggca aauuugaagu gcugaaccgc     3840

auccucaugg ggcugcaugc caaugcuaug auuacagauc uuaaggugcu gggcaucaaa     3900

accaagcugg gacagaugca agucaacggc gggauuaaac ucucccccga cgccaagcug     3960

aucuaucaga gcccuaccgg aauuuucucu agagccgugc ggguuaagga ucuuggcuga     4020


<210>  4139
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4139
augagccuga acaccaauaa gaaauacaac aucggccucg acauugggac aacguccgug       60

ggaugggccg ucaccgaugc ugacggccag aagcugcuua aagccaagaa uaaaaaccug      120

uggggaguuu cucucuucga ggcggggaag acagcugccg aaagacgggg cuaucgcagc      180

accagacggc gccugaauca cagaaaguuu cggcuucgcc ugcucgagga uauguucgaa      240

aaagagaucc uguccaagga ccccucuuuc uucauuagac ugaaagaagc cuuucucagc      300

ccuaaggaug agcaaaaaca guucaagggg aacauccugu uuaaugacaa ggauuacaca      360

gacgcugauu auuacgagca guauaaaacc auuuaccauc uucgguauga ccugaucucc      420

cagcaccgcc aauucgauau cagagaagug uaccucgcca uucaucaccu gaucaaguac      480

cggggacauu uuauuuauga ggaccagaca uucaccacgg auggcaacca gcuucagcac      540

cacaucaaag cuauuaucac caugauuaau ucuauccuga accgcaucau uaucccggaa      600

acaauugaca ucaauguguu ugagaagacc cuccuggaca gaacaaugaa ccggagcucc      660

aaagucaagu uccugauuga gcucaccggc gaagauaaac aagacaagcc ccugcuuaag      720

gagcuguuua aucucaucgu ggggcugaaa gccaagccug cgagcaucuu cgaacaggag      780

aaccuugcca cgauuguuga aacaaugaau augucuaccg aacaggugca gcuggaucuc      840

cugacacugg cugacguccu cgccgaugag gaauacgacc ugcuucugac cgcucagaaa      900

aucuauuccg ccauuauccu cgaugagagc auggacggau acgaauauuu cgcggaggcc      960

aagaaagaau cuuaccgcaa gcaucaggag gagcuggugc uuguuaagaa aaugcugaag     1020

uccaacgcua uuaccaacga ugaaagagcc aaauuugagu auuucuacac agacuacauc     1080

ggcgcuaaga gcucuaauua ugaagagucc aaaaucauua agaaggggcu cagcgccgcc     1140

uacggaaaau auuccaagga agaacggcug uuuaaacaca ucgagcugcu ccuggcuaag     1200

gaaaacgugc uuacgaccgu cgagcaugau cugcucgaga aaaauauuac auucgcgucu     1260

cugcugcccc uucagagaag cucccgcaac gccgugaucc cuuaccaagu gcacgagaag     1320

gagcucgucg cuauucugga aaaucaggcc accuauuacc ccuuucugcu cgagcagaag     1380

gacaacaucc acaaacugcu uacauuccgg auuccuuauu acguuggccc gcuggccgac     1440

cagaaggaua gcgaauuugc uuggauggug agaaaacaag ccgggaagau cacccccuuc     1500

aauuuugagg aaauggugga caucgaugcg ucuuccgagg cuuucauuaa acgcaugacg     1560

aacaagugca cauaccucau ccaugaggac gucauuccua aacacagcuu uucuuaugcc     1620

aaguucgaag ugcugaauga gcugaacaag auccggcuug auggaaaacc cauugacauc     1680

ccucucaaga aaagaaucuu ugaaggccug uuccuggaga agaccaaagu uacccauaca     1740

agccucaaga aguggcuggc cgaauacgag cacaugaccg uguccgucgu gcagggcaca     1800

cagaaagaga cggaauuugc uaccucucuu caggccuuuc aucgcuucgu uaagauuuuu     1860

gaucgggaga cagugagcaa ucccgcuaac gaagagaugu ucgagaaaau cauuuauugg     1920

uccaccgucu uugaggacaa gaaaaucaug agacgcaagc ugagcgagua cccgcaacuc     1980

acagaacagc aacaggugca acuggcccag guccgguuca gagggugggg acgccugucu     2040

cagcggcuua uuaauagaau caagaccccu guguccggcg augaggacca caaacucagc     2100

aucaacgaaa uucuguggca gacgaaugag aacuuuaugc aaaucauucg gaacaaggau     2160

uaucuguuca aaaagaucau ugaggaacag uuugagaaug aaaccgcgcu ccugaacaaa     2220

cagcgcaucg acgagcuugc cacaucuccc gcuaauaaga aggggaucug gcaagccauu     2280

aaaaucguua aggaacugga gaaagugcuc cagcagccug cugagaacau uuucaucgaa     2340

uuugccagau ccgaugagga aagcaagcgg uccaccccca gagacaaauu cauugagaag     2400

gcguacgccc agcugaaaaa ggaaacagac accuuuaauc uggagcaccu uaaggagcuc     2460

aaacaacgcu cuaagcagcu gagcucccag cggcuguucc ucuauuucau ccagaacgga     2520

aaauguaugu acucuggcga acaucuggau auugagagac uugacagcua cgaagucgau     2580

cacauccugc cccaauccua uaucaaggac aauagcauug agaaccucgc ucuggugaaa     2640

aagguugaaa aucagcgcaa gaaagauucu cugcuucuga acuccagcau cauuaaucag     2700

aacuacucua gaugggagca gcucaagaau gccgggcuga ucggagagaa aaaguuucgg     2760

aaccucacgc gcacaaaaau uaccgacaga gauaaggaag gcuucaucgc ucggcaacug     2820

guggagacac gccagaucac caagcauguc acccagcuuc ugcaacagga auauaaagac     2880

acaacgaagg uguuugccau uaaagccacc cucguguccg aucugagacg gaaguucgag     2940

uuuaucaaaa aucgcaacgu uaacgacuac caccacgcuc aggaugcguu ccuggucgcc     3000

uuucuuggca caaauauuac cagcaacuau ccuaagaucg agauggaaua ccuguucaag     3060

ggguaucagc auuaccucaa ugagguggga aaaucugcug ccaagccgaa auuuacauuc     3120

auuguggaaa accugagcaa gcaacaguac aauuccacca cgggcgaggu caaauggaac     3180

cccgaagugg acaucgccaa gcucaagaga auccugaauu uuaaacagug caacauuguu     3240

cggaaggugg aggagcagag cggggcucuu uucaaagaaa caaucuaucc ugucgaggaa     3300

ucuuccagca agaccauucc ccugaaaaag caccucgaua ccgccaucua cggaggcuau     3360

acagcgguga auuacgcuuc cuaugcccug auucaauuca aaaaggggcg caagcugaaa     3420

agagagauca uuggcauccc ucuugccguu cagacccgga ucgacaacuc ugaaacaagc     3480

cugcagacgu acauugcuga gcagaucaag uccgaggugg aaauucucaa uggaagaauc     3540

cugaaauauc aacucauuuc uaacaauggc aaccgccugu acaucgccgg gccccgggag     3600

agacauaaug cgcgccagcu uaucgucagc gacgaagcug ccaaggugau uuggcugauc     3660

uccaccaaac aggccguuga ggcuacauuu cugaagcacu acacccucga acaucuggag     3720

gccguguucg aggaacuuau ucggaagcag gcugccgauu aucaaaucuu ugagaaccug     3780

auuaaaaaga ucgaagucaa caaagcguac uucuauucuu guacauacaa ugagaaggug     3840

aaagugaucg aggaacuccu gaagauuacc caggccaacg cuacgaaugg agaccucaag     3900

cugcuuaaaa ugaccaacag agagggccgc cuggggagcg ucuccguugc ccuggauuuu     3960

aagaucauua aucagagcgu gacaggacuc uaucaaucua ucgaagacua caacaauuga     4020


<210>  4140
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4140
augagccuga acaccaauaa gaaauacaac aucggccucg acauugggac aacguccgug       60

ggaugggccg ucaccgaugc ugacggccag aagcugcuua aagccaagaa uaaaaaccug      120

uggggaguuu cucucuucga ggcggggaag acagcugccg aaagacgggg cuaucgcagc      180

accagacggc gccugaauca cagaaaguuu cggcuucgcc ugcucgagga uauguucgaa      240

aaagagaucc uguccaagga ccccucuuuc uucauuagac ugaaagaagc cuuucucagc      300

ccuaaggaug agcaaaaaca guucaagggg aacauccugu uuaaugacaa ggauuacaca      360

gacgcugauu auuacgagca guauaaaacc auuuaccauc uucgguauga ccugaucucc      420

cagcaccgcc aauucgauau cagagaagug uaccucgcca uucaucaccu gaucaaguac      480

cggggacauu uuauuuauga ggaccagaca uucaccacgg auggcaacca gcuucagcac      540

cacaucaaag cuauuaucac caugauuaau ucuauccuga accgcaucau uaucccggaa      600

acaauugaca ucaauguguu ugagaagacc cuccuggaca gaacaaugaa ccggagcucc      660

aaagucaagu uccugauuga gcucaccggc gaagauaaac aagacaagcc ccugcuuaag      720

gagcuguuua aucucaucgu ggggcugaaa gccaagccug cgagcaucuu cgaacaggag      780

aaccuugcca cgauuguuga aacaaugaau augucuaccg aacaggugca gcuggaucuc      840

cugacacugg cugacguccu cgccgaugag gaauacgacc ugcuucugac cgcucagaaa      900

aucuauuccg ccauuauccu cgaugagagc auggacggau acgaauauuu cgcggaggcc      960

aagaaagaau cuuaccgcaa gcaucaggag gagcuggugc uuguuaagaa aaugcugaag     1020

uccaacgcua uuaccaacga ugaaagagcc aaauuugagu auuucuacac agacuacauc     1080

ggcgcuaaga gcucuaauua ugaagagucc aaaaucauua agaaggggcu cagcgccgcc     1140

uacggaaaau auuccaagga agaacggcug uuuaaacaca ucgagcugcu ccuggcuaag     1200

gaaaacgugc uuacgaccgu cgagcaugau cugcucgaga aaaauauuac auucgcgucu     1260

cugcugcccc uucagagaag cucccgcaac gccgugaucc cuuaccaagu gcacgagaag     1320

gagcucgucg cuauucugga aaaucaggcc accuauuacc ccuuucugcu cgagcagaag     1380

gacaacaucc acaaacugcu uacauuccgg auuccuuauu acguuggccc gcuggccgac     1440

cagaaggaua gcgaauuugc uuggauggug agaaaacaag ccgggaagau cacccccuuc     1500

aauuuugagg aaauggugga caucgaugcg ucuuccgagg cuuucauuaa acgcaugacg     1560

aacaagugca cauaccucau ccaugaggac gucauuccua aacacagcuu uucuuaugcc     1620

aaguucgaag ugcugaauga gcugaacaag auccggcuug auggaaaacc cauugacauc     1680

ccucucaaga aaagaaucuu ugaaggccug uuccuggaga agaccaaagu uacccauaca     1740

agccucaaga aguggcuggc cgaacacgag cauaugaccg uguccgucgu gcagggcaca     1800

cagaaagaga cggaauuugc uaccucucuu caggccuuuc accgcuucgu uaagauuuuu     1860

gaucgggaga cagugagcaa ucccgcuaac gaagagaugu ucgagaaaau cauuuacugg     1920

uccaccgucu uugaggacaa gaaaaucaug agacgcaagc ugagcgagua uccgcaacuc     1980

acagaacagc aacaggugca acuggcccag guccgguuca gagggugggg acgccugucu     2040

cagcggcuua uuaauagaau caagaccccu guguccggcg augaggacca caaacucagc     2100

aucaacgaaa uucuguggca gacgaaugag aacuuuaugc aaaucauucg gaacaaggau     2160

uaccuguuca aaaagaucau ugaggaacag uuugagaaug aaaccgcgcu ccugaacaaa     2220

cagcgcaucg acgagcuugc cacaucuccc gcuaauaaga aggggaucug gcaagccauu     2280

aaaaucguua aggaacugga gaaagugcuc cagcagccug cugagaacau uuucaucgaa     2340

uuugccagau ccgaugagga aagcaagcgg uccaccccca gagacaaauu cauugagaag     2400

gcguaugccc agcugaaaaa ggaaacagac accuuuaauc uggagcaucu uaaggagcuc     2460

aaacaacgcu cuaagcagcu gagcucccag cggcuguucc ucuacuucau ccagaacgga     2520

aaauguaugu auucuggcga acaccuggau auugagagac uugacagcua cgaagucgau     2580

cauauccugc cccaauccua caucaaggac aauagcauug agaaccucgc ucuggugaaa     2640

aagguugaaa aucagcgcaa gaaagauucu cugcuucuga acuccagcau cauuaaucag     2700

aacuauucua gaugggagca gcucaagaau gccgggcuga ucggagagaa aaaguuucgg     2760

aaccucacgc gcacaaaaau uaccgacaga gauaaggaag gcuucaucgc ucggcaacug     2820

guggagacac gccagaucac caagcacguc acccagcuuc ugcaacagga auacaaagac     2880

acaacgaagg uguuugccau uaaagccacc cucguguccg aucugagacg gaaguucgag     2940

uuuaucaaaa aucgcaacgu uaacgacuau caccaugcuc aggaugcguu ccuggucgcc     3000

uuucuuggca caaauauuac cagcaacuac ccuaagaucg agauggaaua ucuguucaag     3060

ggguaccagc acuaucucaa ugagguggga aaaucugcug ccaagccgaa auuuacauuc     3120

auuguggaaa accugagcaa gcaacaguac aauuccacca cgggcgaggu caaauggaac     3180

cccgaagugg acaucgccaa gcucaagaga auccugaauu uuaaacagug caacauuguu     3240

cggaaggugg aggagcagag cggggcucuu uucaaagaaa caaucuacuc ugucgaggaa     3300

uccagcucca agaccauucc ucugaaaaag caucucgaua ccgccaucua uggaggcuac     3360

acagcgguga auuaugcuuc uuacgcccug auucaauuca aaaaggggcg caagcugaaa     3420

agagagauca uuggcauccc ccuugccguu cagacccgga ucgacaacag cgaaacaucc     3480

cugcagacgu auauugcuga gcagaucaag ucugaggugg aaauucucaa uggaagaauc     3540

cugaaauacc aacucauuag caacaauggc aaccgccugu auaucgccgg gccucgggag     3600

agacacaaug cgcgccagcu uaucgucucc gacgaagcug ccaaggugau uuggcugauc     3660

ucuaccaaac aggccguuga ggcuacauuu cugaagcauu acacccucga acaccuggag     3720

gccguguucg aggaacuuau ucggaagcag gcugccgauu accaaaucuu ugagaaccug     3780

auuaaaaaga ucgaagucaa caaagcguau uucuacagcu guacauauaa ugagaaggug     3840

aaagugaucg aggaacuccu gaagauuacc caggccaacg cuacgaaugg agaccucaag     3900

cugcuuaaaa ugaccaacag agagggccgc cugggguccg ucagcguugc ccuggauuuu     3960

aagaucauua aucagucugu gacaggacuc uaccaaucca ucgaagacua uaacaauuga     4020


<210>  4141
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4141
augaagaaag aguacaccau cggccuggac auugggacaa acagcguggg augguccguc       60

cucacggaug acuauagacu gguuucuaag aaaaugaagg uggccggcaa uaccgaaaag      120

agcuccacaa agaagaacuu cuggggagug cggcuuuuug augagggaca gaccgcugaa      180

gcccgcagau cuaaacggac agcgcgcaga cggcuggcuc gcagacggca acgcauccuc      240

gagcugcaga agauuuucgc ccccgaaauc cuuaaaauug acgagcacuu uuucgccaga      300

cugaaugaga gcuuucucgu ccuggaugaa aagaaacagu cccggcaucc uguguucgcu      360

accaucaagc aggagaaguc uuaccaccaa acauauccga ccaucuacca ucugcgccag      420

gcccucgcug acagcuccga aaaagccgau auuagacugg uuuaucuugc gauggcccau      480

cugcucaagu accggggcca cuuucugauc gagggggagc uuaacacgga aaauagcucu      540

gugaccgaga cauuccgcca guuucugucc accuauaacc agcaauucag cgaagcugac      600

gauaaacaga cagagaagcu cgacgaagcc gucgauugcu cuuuuguguu caccgagaaa      660

auguccaaga cgaaaaaggc ugagacacug cugaaguacu uuccccacga aaagagcaau      720

ggauaccucu cucaguucau uaagcugaug guuggcaacc agggcaauuu caaaaacgug      780

uuugggcuug aggaagccaa gcugcaauuc uccaaagaga ccuaugaaga ggaccucgag      840

gaacugcuug agaagaucgg agaugacuac auugaccugu uuguccaggc gaagaaugug      900

uaugaugccg ugcuccugag cgaaauccug uccgacucua ccaaaaacac aagagcuaag      960

cucagcgccg gcaugauucg gagauacgau gcucacaaag aggaccuggu ccuucugaag     1020

cgcuucguua aagaaaaucu cccuaagaag uaucgggccu uuuucgggga uaacuccgug     1080

aauggauacg ccggcuauau cgaggggcau gcuacccagg aggacuuuua caaauucgug     1140

aagaaagaac ugacaggaau cagaggcagc gaggucuuuc uuacgaagau ugaacaagag     1200

aacuuccugc gcaaacagcg gaccuuugau aacggcguga ucccccacca gauucaucuc     1260

acagagcuga gagcgaucau ugccaaucag aagaagcacu acccuuuccu gaaagaggaa     1320

caagaaaagc ucgagucucu gcuuaccuuu aaaauccccu auuacguugg gccucuggcu     1380

aagaaacagg aaaacucccc guucgccugg cucauccgca agagcgagga gaaaauuaag     1440

cccuggaauc ugccugaaau cguggacaug gagggaucug ccguccgguu cauugaaaga     1500

augaucaaca cagauaugua uaugccccac aauaaggugc ugccuaaaaa cagccuucuc     1560

uaccagaagu uuuccauuua uaaugagcug accaaaguuc gcuaccagga cgaacggggc     1620

caaaugaacu auuucucuag caucgagaag aaagagaucu uucaugaacu guucgagaag     1680

aauagaaagg ugacgaaaaa ggaucuccag gaauuucugu accuuaaaua cgacauuaag     1740

cacgcugagc uguccgggau cgaaaaagcc uucaacgcga gcuauacaac cuaccaugac     1800

uuucucacca ugucugagaa uaagcgcgag augaagcagu ggcuggaaga ucccgagcug     1860

gcuuccaugu ucgaagagau uaucaaaaca cuuaccgucu uugaggaccg ggaaaugauu     1920

aagacaagac ucagccacca ugaggccacg cugggaaaac acaucauuaa gaaacugacc     1980

aagaagcacu auacaggcug ggggcggcuc ucuaaagaac ugauccaggg aauccgcgau     2040

aagcaaucca acaaaaccau ucuugacuac cugaucaaug augacgauuu cccgcaucac     2100

agaaaccgga auuuuaugca gcucauuaac gacgauagcc uguccuucaa gaaagagauc     2160

aagaaagccc agaugauuac agacaccgaa aaccuggagg agaucgugaa ggaacuuacg     2220

ggcucuccug cuaucaagaa aggcauucuc cagagccuga agaucgucga ugagauugug     2280

gggaucaugg gauaugaacc cgccaauauu guuguggaga uggcuagaga aaaccaaacc     2340

acaggccgcg ggcugaaauc cucucggccu agacucaagg cccuggagga gagccuuaaa     2400

gacuuuggau cccagcugcu caaggaauac cccaccgaua auagcucucu gcagaaggac     2460

cgccuguauc uuuacuaccu ccaaaacggc agagacaugu auacaggggc gccccuggau     2520

auccaucggc uguccgacua cgauaucgac cacauuaucc cucgcagcuu caccacggau     2580

aauucuauug acaacaaagu ccucgugucc agcaaggaga auagacugaa aaaggaugac     2640

guuccgucug aaaaaguggu caagaagaug cggagcuucu gguaugaucu uuacuccagc     2700

aaacugaucu cuaagcgcaa acucgacaac cugacaaaga uuaaacugac cgaggaagau     2760

aaggccggcu uuaucaagag acagcuugug gagacacggc agaucaccaa acacguggcu     2820

gggauucugc aucaccgcuu caauaaggcc gaggacacca acgaacccau cagaaaaguu     2880

cggauuauca cacucaaguc cgcucugguc agccaguuuc gcaauagauu cggcauuuau     2940

aaagugcggg agaucaacga auaccaucac gcccaugaug cguaucucaa uggggugguc     3000

gcccuggccc uucugaagaa guacccucaa cucgcucccg aguuugugua cggagaguau     3060

cugaaauuca acgcucacaa ggccaauaaa gccacgguua agaaagaauu uuacuccaac     3120

auuaugaagu ucuuugaguc ugacaccccu gugugugacg aaaacggcga gaucuucugg     3180

gauaaaagca aguccaucgc ucaggucaag aaagugauua aucaccauca caugaacauc     3240

guuaagaaaa cagaaauuca gaaaggggga uuuucuaaag agaccgugga gcccaagaag     3300

gacagcucca aacugcuucc gagaaagaau aacugggauc cugcgaaaua uggcgggcug     3360

ggcucuccca augucgccua cacaguggcu uucaccuaug aaaagggaaa agcccgcaag     3420

cggacgaacg cccucgaggg caucacaauu auggaaagag aggcuuucga acagagcccu     3480

guucuguuuc ucaagaauaa aggguacgag caagccgaga ucgaaaugaa gcugcccaaa     3540

uaugcgcuuu ucgagcugga aaacggacgc aagcggaugg uggcuuccaa uaaagaggcc     3600

cagaaggcca acagcuuucu ccugccugaa caucugguca cccuucugua ccacgcuaag     3660

caguacgacg agaucucuca uaaagagucc uucgauuaug ugaaugaaca ccacaaggag     3720

uuuagcgaag uguucgccag aguccucgag uuugcgggca aauacacccu ggcugagaag     3780

aacauugaaa aacucgagaa gaucuauaaa gaaaaucaga cagacgaucu ggccaaacuu     3840

gccucuuccu ucguuaaccu gaugcaauuu aaugcuaugg gggcccccgc ugacuucaag     3900

uuuuucgaug ugaccauucc gcgcaaacgg uacacaagcc ugacggagau cuggcagucu     3960

accauuaucc aucaguccau cacaggacuc uaugaaaccc gcauuagaau gggcaaguga     4020


<210>  4142
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4142
augaagaaag aguacaccau cggccuggac auugggacaa acagcguggg augguccguc       60

cucacggaug acuauagacu gguuucuaag aaaaugaagg uggccggcaa uaccgaaaag      120

agcuccacaa agaagaacuu cuggggagug cggcuuuuug augagggaca gaccgcugaa      180

gcccgcagau cuaaacggac agcgcgcaga cggcuggcuc gcagacggca acgcauccuc      240

gagcugcaga agauuuucgc ccccgaaauc cuuaaaauug acgagcacuu uuucgccaga      300

cugaaugaga gcuuucucgu ccuggaugaa aagaaacagu cccggcaucc uguguucgcu      360

accaucaagc aggagaaguc uuaccaccaa acauauccga ccaucuacca ucugcgccag      420

gcccucgcug acagcuccga aaaagccgau auuagacugg uuuaucuugc gauggcccau      480

cugcucaagu accggggcca cuuucugauc gagggggagc uuaacacgga aaauagcucu      540

gugaccgaga cauuccgcca guuucugucc accuauaacc agcaauucag cgaagcugac      600

gauaaacaga cagagaagcu cgacgaagcc gucgauugcu cuuuuguguu caccgagaaa      660

auguccaaga cgaaaaaggc ugagacacug cugaaguacu uuccccacga aaagagcaau      720

ggauaccucu cucaguucau uaagcugaug guuggcaacc agggcaauuu caaaaacgug      780

uuugggcuug aggaagaggc caagcugcaa uucuccaaag aaaccuauga ggaggaccuc      840

gaggaacugc uugaaaagau cggagaugac uacauugacc uguuugucca ggcgaagaau      900

guguaugaug ccgugcuccu gagcgagauc cuguccgacu cuaccaaaaa cacaagagcu      960

aagcucagcg ccggcaugau ucggagauac gaugcucaca aagaagaccu gguccuucug     1020

aagcgcuucg uuaaagagaa ucucccuaag aaguaucggg ccuuuuucgg ggauaacucc     1080

gugaauggau acgccggcua uaucgagggg caugcuaccc aggaagacuu uuacaaauuc     1140

gugaagaaag agcugacagg aaucagaggc agcgaagucu uucuuacgaa gauugagcaa     1200

gagaacuucc ugcgcaaaca gcggaccuuu gauaacggcg ugauccccca ccagauucau     1260

cucacagaac ugagagcgau cauugccaau cagaagaagc acuacccuuu ccugaaagag     1320

gaacaagaga agcucgaauc ucugcuuacc uuuaaaaucc ccuauuacgu ugggccucug     1380

gcuaagaaac aggagaacuc cccguucgcc uggcucaucc gcaagagcga ggagaaaauu     1440

aagcccugga aucugccuga aaucguggac auggaaggau cugccguccg guucauugag     1500

agaaugauca acacagauau guauaugccc cacaauaagg ugcugccuaa aaacagccuu     1560

cucuaccaga aguuuuccau uuauaaugaa cugaccaaag uucgcuacca ggacgagcgg     1620

ggccaaauga acuauuucuc uagcaucgag aagaaagaaa ucuuucauga gcuguucgaa     1680

aagaauagaa aggugacgaa aaaggaucuc caggaguuuc uguaccuuaa auacgacauu     1740

aagcacgcug aacuguccgg gaucgagaaa gccuucaacg cgagcuauac aaccuaccau     1800

gacuuucuca ccaugucuga gaauaagcgc gaaaugaagc aguggcugga ggaucccgaa     1860

cuggcuucca uguucgagga gauuaucaaa acacuuaccg ucuuugaaga ccgggagaug     1920

auuaagacaa gacucagcca ccaugaagcc acgcugggaa aacacaucau uaagaaacug     1980

accaagaagc acuauacagg cugggggcgg cucucuaaag agcugaucca gggaauccgc     2040

gauaagcaau ccaacaaaac cauucuugac uaccugauca augaugacga uuucccgcau     2100

cacagaaacc ggaauuuuau gcagcucauu aacgacgaua gccuguccuu caagaaagaa     2160

aucaagaaag cccagaugau uacagacacc gagaaccugg aggaaaucgu gaaggagcuu     2220

acgggcucuc cugcuaucaa gaaaggcauu cuccagagcc ugaagaucgu cgaugaaauu     2280

guggggauca ugggauauga gcccgccaau auuguugugg aaauggcuag agagaaccaa     2340

accacaggcc gcgggcugaa auccucucgg ccuagacuca aggcccugga ggaaagccuu     2400

aaagacuuug gaucccagcu gcucaaggag uaccccaccg auaauagcuc ucugcagaag     2460

gaccgccugu aucuuuacua ccuccaaaac ggcagagaca uguauacagg ggcgccccug     2520

gauauccauc ggcuguccga cuacgauauc gaccacauua ucccucgcag cuucaccacg     2580

gauaauucua uugacaacaa aguccucgug uccagcaagg aaaauagacu gaaaaaggau     2640

gacguuccgu cugagaaagu ggucaagaag augcggagcu ucugguauga ucuuuacucc     2700

agcaaacuga ucucuaagcg caaacucgac aaccugacaa agauuaaacu gaccgaagag     2760

gauaaggccg gcuuuaucaa gagacagcuu guggagacac ggcagaucac caaacacgug     2820

gcugggauuc ugcaucaccg cuucaauaag gccgaagaca ccaacgagcc caucagaaaa     2880

guucggauua ucacacucaa guccgcucug gucagccagu uucgcaauag auucggcauu     2940

uauaaagugc gggaaaucaa cgaguaccau cacgcccaug augcguaucu caauggggug     3000

gucgcccugg cccuucugaa gaaguacccu caacucgcuc ccgaguuugu guacggagaa     3060

uaucugaaau ucaacgcuca caaggccaau aaagccacgg uuaagaaaga guuuuacucc     3120

aacauuauga aguucuuuga aucugacacc ccugugugug acgagaacgg cgaaaucuuc     3180

ugggauaaaa gcaaguccau cgcucagguc aagaaaguga uuaaucacca ucacaugaac     3240

aucguuaaga aaacagagau ucagaaaggg ggauuuucua aagagaccgu ggaacccaag     3300

aaggacagcu ccaaacugcu uccgagaaag aauaacuggg auccugcgaa auauggcggg     3360

cugggcucuc ccaaugucgc cuacacagug gcuuucaccu augagaaggg aaaagcccgc     3420

aagcggacga acgcccucga aggcaucaca auuauggaga gagaggcuuu cgaacagagc     3480

ccuguucugu uucucaagaa uaaaggguac gagcaagccg aaaucgagau gaagcugccc     3540

aaauaugcgc uuuucgaacu ggagaacgga cgcaagcgga ugguggcuuc caauaaagaa     3600

gcccagaagg ccaacagcuu ucuccugccu gagcaucugg ucacccuucu guaccacgcu     3660

aagcaguacg acgagaucuc ucauaaagaa uccuucgauu augugaauga gcaccacaag     3720

gaauuuagcg agguguucgc cagaguccuc gaguuugcgg gcaaauacac ccuggcugaa     3780

aagaacauug agaaacucga aaagaucuau aaagagaauc agacagacga ucuggccaaa     3840

cuugccucuu ccuucguuaa ccugaugcaa uuuaaugcua ugggggcccc cgcugacuuc     3900

aaguuuuucg augugaccau uccgcgcaaa cgguacacaa gccugacgga aaucuggcag     3960

ucuaccauua uccaucaguc caucacagga cucuaugaga cccgcauuag aaugggcaag     4020

uga                                                                   4023


<210>  4143
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4143
augaagaccg acuacuucau gggccuggau auggggacag gaagccucgg cugggccgug       60

acggacuccu cuuaucaccu gugcagagcu caugggaaag aucuuugggg aguccggcug      120

uuugagaccg cccagacagc ggaagaacgc agaguuuucc gguguaaccg cagacggcuc      180

gaccgcagaa agcggcgcau ccaacugcuu caggauauuu uugcucccga aaucuacaaa      240

auugacccug gcuucuuucu gagacucaag gagagccggu aucugccgga agauaaaaag      300

aaucuggagg ggaagacccc cgagcucccu uacgcccugu ucguggacaa aaaguauaca      360

gauaaagacu uucacaagga auaccccacc aucuaucauc uucgccugag acucaugcag      420

gaucggaccc cugccgacgu gcgccugguc uaccuugcuc ugcaccaucu cauuaaacac      480

agaggacacu uccuguuugc cggcaucgag gcugauaagg ugacagacuu cuccgccgcg      540

uuuucugagu uccugcggau cguucaggag caagagcucg acauuagcuu uacgcccgau      600

gaagaguccu cucagaucau uaaggaaacc cugcagaaca caaauuucac cagaagcaaa      660

aaguccacag agcuuaucaa aggccugaag gccagcaccg ggugcgaaaa agcucuccug      720

aaacuuauuu cuggauguaa agugaagcug uccgccgucu ucgacgaucc uggcugcgac      780

gcuuacgaga agccccaggu guguuuugcc gagagcucuu augaagauua cgcggccauc      840

cucgaggacg aacuggagga ucgcuucauu guuaucgcug ccgcuaaagc ccuguaugac      900

ugguccgugc ucgucaacau ccugggggaa gccagcacgg ugucugaggc uaagguggga      960

auuuacgaga aacaucggag agaucuuaag uaucugaaac accucgucaa gacaaaucug     1020

acccgcgaag acuaccaaga ucuuuuuguu aaggcgggcg ccaaaaagaa caauuauugc     1080

gcuuacaucg ggaugacaaa gaagaacaau aaaaaggugg ugcuggagga aaagcggugu     1140

accaaagagg acuucuacgc cuaucucaag aaagaaauuu acuccaaggu ccaggagaac     1200

ccggaggccg aauaucugcu ggaggaacuc gagaaaggaa ccuuucugcc uagacaggug     1260

agcaaggaca auggcguuau ccccuaccag cuucaauucc uggagcucca gaagauucug     1320

aaaaacgcug uggaauaucu gccuuuucuu aaggaggaau ccgagaaaau ccagcaaauc     1380

augacauucc gcauucccua cuaugucggc ccgcucaaug ggaagaaaga aggacagaac     1440

acguuugccu gggcggugcg gaagaauaac gagccuguuu accccuggaa cuucgaggaa     1500

gugaucgaua ccgaggcuuc ugccgaacag uuuauuagac gcaugacaaa uaaaugcacc     1560

uaccugccug gcgaggacgu ccugcccaag gaaagccucc uguauuccaa guucauggug     1620

cuuaacgagc ugaauaaccu ccggaucaau gggacaaaaa uuagcgucga gcugaagcag     1680

gaacuguacg agaaacuuuu ucaaagauau cgcaaggugu cugccaaaaa gcuccgggau     1740

uaccugguua gaaacggaau caccgaaaag gacgcugaga ucuccggcau ugauggggac     1800

uucaaaggaa cgcugcgggc cuaucaugau uucaaggaaa aacucacagg cguggagcug     1860

accgaggcug aaaaggagac caucauucuu aauaucacac uguuuggcga agacaaaguc     1920

cuccugaaga agcgcaugca gagacugcuu cccaaccuca ccgaggggca gaaaaaggcc     1980

auugugacac ugaauuacag cggauggggc cggcugucua gagaguuccu cgaagagguu     2040

caggcgccua acccggaaag cggggagauc cuguccauua uccgcgcccu uugggaaacg     2100

aaugcuaacc ugaugcaacu ccugucuaau caguauggau accaggagau ccuggagcag     2160

augcgggccg auuggaccgg ccacaaacuu gacuaccaaa caguggagga acucuauguc     2220

agccccgcug ugaagagaca gauuuggcag acccuccagg ugguuaaaga acugaagaag     2280

gucaugggcg gggagccuaa acgcguguuu cucgaagugg ccagagagaa gauggagucc     2340

ggacggacag ucagccgcaa aaagcaccug aaagaucuuu accugucuug uaaguccgaa     2400

gagagaaacu ggcucgcgga acuggaccgg caugaggaac acagcuaucg cucugauaga     2460

cuguuccuuu acuauaccca aaaaggccgg ugcauguacu gugggaagcc caucgaccug     2520

ggacaucucu gggacgugaa uauguaugau auugaccaca ucuacccuca guccaagguu     2580

auggaugaca gcauucgcaa cagagugcug acgugccgga ccugcaacgc cgagaaaaca     2640

gaucauuacc cccucaaggc ugagauccgc cagaaaauga gaccguuuug ggacucccug     2700

aaggccggca auuucaucga agaggaaaaa uggaagcggc uuauuagauc ugaggaguuu     2760

accagcacag aacuggcugg guucaucgag cgccaacucg ucgaaacccg gcaguccacg     2820

aaggccgugg ccgauauucu gaaacaggcu augccugaga cagaaaucgu uuaugugaag     2880

gcgaaaaacg ucucugccuu uagacaggac uucaauuuug ugaagaaccg cgauguuaau     2940

gacuuccacc acgcuaaaga ugccuaccug aacauugugg ucggaaauac cuauuuugug     3000

aaguucacaa agaacgccag cugguucauc gagcggaauc ccggcagauc cuacaaccuu     3060

aaacgcaugu uuaccagcga cuaugaugug augucuaaug gggagaucgc uuggaaggcc     3120

ggcaacuccg gaaccauuac aacgguccgg aaaaugauga agaaaaauag cguucuguuc     3180

accagacgcu cuuuugaagu guccggcggg cuguucgacc aacagcugau gaagaaggga     3240

aaaggccagg uguacgucaa gagcggggac gagcggcuca gaucuaucga aaaauaugga     3300

ggcuacaaca aggcgacagg ggcuuacuuu augcuggugg agagcgaagg caaaaaggga     3360

guuaagaaac ggaccauuga guucgugccu cuuuaucgca aggccgagcu ggaagccucc     3420

gaggagaaaa ugcucgaaua ccugagaaca gaaaucggcc ugaaggagcc ccauguccuu     3480

cugcacaaaa uuaagaccga uacgcucuuu caggcugacg gguucaaaau gcaucugagc     3540

ggacggacag gcaagcaacu cguguuuaag ggggccaauc aguuccuggu ugcggaggau     3600

uaugaacgcc uucugaaaaa ggugggaaaa gucaccgagc agcugaagac caacaaaaac     3660

uacaagauca augacuauga uggcaucaca gaagaggggc ucaaggcucu guacucugag     3720

uuucuuucca aacugcagaa ggccccuuau ggcaacagac ucggaaccca agccgaaaca     3780

cuggagaaug gcaaagaaaa guucgacaaa cucagccugg agaacaagug ucaggugcuu     3840

uccgaaauuc ugcaccuguu ccagugcaau ucuagcgcug ccgaucucuc caagcuggga     3900

gggcccggcc acgggggaau ccuuguucug ucuaacgaga uuacgaaaug uaauaagauc     3960

agcauuaucc aucaaucccc ggcuggcauu uacgagcagg aaaucgaccu caaaacccug     4020

uga                                                                   4023


<210>  4144
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4144
auggagcagg aauacuaucu gggccucgac auggggaccg gaagcguggg cugggccguc       60

acagauuccg aguaccacgu ucugagaaag caugggaaag cucuuugggg agugcggcug      120

uucgaaucug ccagcacggc ggaggaacgc agaauguuuc ggaccucccg cagacggcuc      180

gaccgcagaa acuggcggau cgagauucug caagagaucu ucgcugaaga gauuucuaag      240

aaagaucccg gcuuuuuccu ucgcaugaag gaaagcaaau auuacccuga ggacaagaga      300

gauaucaaug ggaacugccc ggagcugccc uaugcccucu uuguggacga ugacuucaca      360

gauaaggacu accacaaaaa guuuccuacc auuuaucauc ugcggaaaau gcugaugaau      420

acagaagaga cccccgauau ccgccucguc uaccuggcca uccaccauau gaugaagcac      480

agaggacacu uccuucuguc cggcgacauu aacgaaauca aagaguuugg cacaaccuuc      540

ucuaagcucc uggaaaauau uaagaacgag gagcuugauu ggaaucugga acucgggaaa      600

gaggaauacg cugugguuga gagcauccug aaggacaaca ugcugaaucg guccacgaaa      660

aagacccgcc ucauuaaagc ccugaaggcu aaaagcaucu gugaaaaggc cgugcuuaac      720

cugcucgcgg gaggcacagu caagcugucu gacauuuuug ggcuugagga gcugaaugaa      780

accgagagac cuaaaaucuc cuucgccgau aacggauaug acgauuacau cggcgaagug      840

gagaaugaac ucggggagca guuuuauauu aucgagacag cuaaggccgu uuacgacugg      900

gcugugcugg ucgaaauucu gggaaaauau accagcaucu cugaggccaa gguggcgacg      960

uacgaaaaac auaaguccga ucuccaguuc cugaagaaaa uugugcggaa guaucuuaca     1020

aaagaggagu acaaggacau cuucgucagc accucugaua aacugaagaa cuacuccgcc     1080

uauaucggca ugacaaagau uaauggcaaa aagguugacc uccagagcaa aagaugcucc     1140

aaggaagagu uuuacgauuu caucaaaaag aacgugcuga agaaacuuga agggcaaccc     1200

gaguaugaau accugaagga ggagcucgaa cgcgagaccu uucugccuaa acaggugaac     1260

cgggacaaug gagucauucc guaucagauc caccuguacg aacucaagaa aauucugggc     1320

aaccuuagag auaagaucga ccugaucaaa gagaaugaag acaagcucgu gcagcuguuc     1380

gaguuucgca uucccuauua cguugggccu cugaacaaga ucgaugacgg aaaagagggc     1440

aaguucaccu gggcugugcg gaaaucuaau gaaaagauuu accccuggaa cuuugagaau     1500

gucguggaua ucgaagccag cgcugagaaa uucauuagac gcaugacaaa caaguguacg     1560

uaucuuaugg gggaagacgu ucucccuaag gauucccugc uguacagcaa auauauggug     1620

cucaaugagc ugaacaaugu caaacuugac ggagagaaac ugucugugga acucaagcaa     1680

cggcuguaca ccgaugucuu uugcaaauau agaaagguga caguuaagaa aaucaagaac     1740

uaccugaaau gugagggcau uaucuccggc aauguggaaa ucaccgggau ugacggagau     1800

uucaaggcca gccuuacagc cuaucaugac uuuaaagaga uccucaccgg cacggagcug     1860

gcuaagaagg auaaagaaaa cauuaucaca aauauugucc uguucgggga cgauaagaaa     1920

cuccugaaga aacgccuuaa ccggcuguac ccccagauca ccccgaacca gcucaagaaa     1980

aucugcgcgc ugucuuacac cggauggggc agauuuagca agaaguuccu ggaggaaauu     2040

acagccccug accccgagac cggggaagug uggaauauca uuacagcucu uugggagucc     2100

aacaauaacc ucaugcagcu gcugucuaau gaguaucgcu ucauggaaga gguugaaacg     2160

uacaacaugg gcaaacaaac caagacacuc agcuaugaga ccguggaaaa uauguacguc     2220

uccccuagcg ugaaacggca gaucuggcag acacugaaga uugugaaaga gcuugagaag     2280

guuaugaagg aaucucccaa aagagucuuu aucgagaugg cccgggaaaa gcaagagucc     2340

aaacgcaccg aaagcagaaa gaaacagcug aucgaccucu auaaggccug uaagaacgag     2400

gagaaagauu gggugaagga acugggagac caggaggaac agaaacugcg gucugauaaa     2460

cuuuaccucu auuacacgca aaagggcaga ugcauguacu ccggggaggu gauugagcug     2520

aaggaccugu gggauaauac caaguaugac aucgaucaca uuuaccccca gagcaaaaca     2580

auggacgauu cccucaacaa ucgcguccug gugaagaaaa aguauaacgc uaccaaaucu     2640

gacaaguacc cucuuaauga aaacauccgg cacgagagaa agggauucug gaaaagccug     2700

cucgauggcg gguuuauuuc caaggaaaaa uaugagcgcc ugaucagaaa uacagaacug     2760

ucuccggagg agcuugccgg auucaucgaa cggcagauug uugagacccg ccagagcacg     2820

aaggcggugg cugaaauccu gaaacaaguc uuucccgagu ccgaaauugu guacguuaag     2880

gccggcacag ugagcagauu ccggaaagac uuugagcucc ugaagguccg cgaggugaac     2940

gaucuccauc acgccaagga cgcuuaucug aacaucguug ugggcaauuc uuacuacguc     3000

aaauucacca agaacgccuc cugguuuauu aaagaaaauc cugggagaac auauaaccuu     3060

aagaaaaugu ucaccagcgg auggaauauc gagcggaacg gcgaaguggc uugggaggug     3120

gggaagaagg gaaccauugu cacaguuaaa cagaucauga auaagaacaa uauccuggug     3180

acgcgccagg ugcaugaagc caaaggcggg cucuuugacc agcaaauuau gaagaaagga     3240

aagggccaga ucgcgauuaa ggagaccgau gagagacugg ccucuaucga aaaauacggg     3300

ggcuauaaca aggcugccgg agcuuacuuc augcuggucg aguccaaaga caagaaaggc     3360

aagacaauuc ggaccaucga auuuaucccc cuuuaucuga agaauaaaau ugagagcgau     3420

gagucuaucg cccucaacuu ccuggaaaag gggcgcggac ucaaagagcc uaagauucug     3480

cuuaaaaaga ucaaaauuga cacacuguuc gauguggacg gcuuuaagau guggcucagc     3540

gggagaaccg gagaucggcu gcuguucaag ugugcgaauc agcuuauccu ggacgaaaag     3600

aucauuguua cgaugaagaa aaucgugaag uuuauucaga gacgccaaga gaaccgggaa     3660

cucaaacugu ccgauaagga cggcaucgau aaugaggucc ucauggagau uuacaacaca     3720

uucguggaca agcuggaaaa caccguuuau agaauccgcc uuagcgagca ggccaaaacc     3780

cugauugaca agcagaaaga auuugagcgg cugucucucg aagauaaguc cagcacacug     3840

uucgagaucc uucacaucuu ucaaugccag uccucugcug ccaaucugaa aaugauuggg     3900

ggccccggaa aggcuggcau ccucgugaug aacaauaaca uuagcaagug uaauaaaauc     3960

uccauuauca accagucucc gaccgggauc uucgagaaug aaauugaccu gcucaagauc     4020

uga                                                                   4023


<210>  4145
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4145
augggcgagg aauacuuccu ggggcucgac augggaaccg gcagccuggg gugggccgug       60

acagaugaca aguaucagau ccuuagacgg cacggaaacg cucugugggg cguccgccuc      120

uuugagacgg ccaauaccgc ggaagagaga cggauguucc gcacaaacag acggcgccug      180

gauagacaag gguggcggau uaaaauccuu caggaacugu uugcugagga gauuugccgc      240

guugaccccg gauucuaccu cagaaugaag gaauccaaau auuacccuga ggauaagcgg      300

gacgccaaug gcgaaugucc ggcccugccc uaugcucugu uugccgaugc ucaguacacc      360

gacaaagauu aucaucgcga guucccuaca aucuaccacc ucagaaagau gcugauggag      420

accgaaacaa cccccgacau ucggcuugug uaccuggccu uucaucacau gaugaagcau      480

cgcggccacu uccuccuguc uggggauauc agccagguga cggaguuuag aaacaccuuc      540

cuucaaauga ucgcgaauau uaaauccgaa gaacuggacu uuucucucga aaucgaggag      600

acacagaugg gacagauuga agccguccug aaggaucaca aacugacccg gagcgagaag      660

aaaagacgcc ucacacagau ccugaagccu gcuaccgccu gcgaaaaggc uauuuucacg      720

cuucuguccg gcgggacagu gaaacucucu gacaucuuug gaaaggccga gcuggacgaa      780

ggcgagcggc ccaaaauuag cuucgcggau aacggguaug acgauuacuc cgccagcguu      840

gaggcugacc uuggagaaca auauuacauc aucgagucug ccaaggcugu guaugauugg      900

uccguccuga gcgacauucu cggcgauucu guguccauca gcgacgccaa aguuaagcug      960

uacgaaaaac auaagaagga ucuggccuau cucaaaaagg uggucagaga gaaucugccu     1020

cgcgaagugu acaaccaggu guucgucaau accgacgaga aacuuuguaa cuacugcgcu     1080

uauauuggca uggcgaagaa aaaugggcgg aagguugccc uggagggaaa gagauguaca     1140

cgcgaagagu uugaaucuuu ccuccggaaa aagaucguga acaaaauuga ggauaaggag     1200

auccaggaau accuguccga ggaaaucgag aaaggcaccu uucuuccgag acaggugacc     1260

aaggacaaug gggucauucc cuaucaaauc caccuguacg aacuccgcaa gauuaucgac     1320

aaccugagcc cucggaugcc cuucauuaga gauaauggag acaaaaucau ccagcuguuu     1380

acauuccgca uuccuuauua cgugggcccc cucgggaagg gagagggcuc cgagaaauuu     1440

ucuugggcug uucggagaac ggaugaagcc aucuauccgu ggaacuucaa ggacgugauu     1500

gaugucgagg ccagcgcuga aaaauuuauc ugccggauga ccaacaagug uacauaccug     1560

uacggcgagg acgugcuucc uaaggauagc ccccuguauu ccaaauucau gguucucaau     1620

gaacugaaca aucugaagcu cgacgggcag ucuguguccg ucgagcucaa acaacgcauu     1680

uaccaggagc uguuuugcca uaccagaaag gugacacaga aaaagcuggu cagcuaucuc     1740

gugcgggaag gaaucaccgg caaaaacguu gagauuucug ggaucgaugg agacuucaag     1800

gccagccuga cggcguacca cgauuuuaag gaaaaacuua caggcgugga gcugucccag     1860

caagaacagg aggagaucgu cagaaauauu gugcuuuucg gggaagacaa gaaacugcug     1920

aagcagcgcc uugagggccg guucccucag cucacccaaa aacagaucuc uguuauugcu     1980

acccugagcu auaagggaug gggcagacug ucccgcgaau uucucgagaa caucacagug     2040

cccgcccccg ggaccggaga ggucuggaau auuaucacag cccuguggga aacgaacgau     2100

aaucuuaugc agcugcucua cggcgaguau ggguuccaga ccgaaaucga guguuuuaac     2160

gcuaaggaga aaaagaaaag ccugucuuac gagacaauug aggaauccua cgugagcccu     2220

ucugugaaga gacaaaucug gcagacccug cagguuguca aagagcuuaa gaaggugaug     2280

cggggagacc cgaaacgcgu guucauugaa auggccagag agaaucagga auccaagcgg     2340

acagagagcc gcaaaaagac ccuccuggac cuguauaaaa acugcaagaa ggaggaaccc     2400

gaguggaucg cugaacucac ggagcaccug gaaaaacuug augacuccca acugagaaag     2460

gauaaacucu uucuguacua uacccagaag ggccggugua uguacucugg ggagcgcauu     2520

gagcuggaag accuuuggga uaauacaaga uaugacaucg aucauaucua cccucagagc     2580

aaaaccaugg acgauuccgu cgacaaccgg gugcucguua agaaagagua uaaugccaag     2640

aagacagaug uguaccccau ugcggccgac auccgcggca aaaugucucc uuucuggaag     2700

aaacugcugg auaacaagcu cauuagcaaa aagaaguacg acagacuggu ccggaccgaa     2760

gaacuuacgc ccgaggaacu ggcuggauuu aucgacagac aacucgugga gacacgccag     2820

uccaccaaag uuguggccga aauucugaag gaggcuuucc cggauacaga aaucgucuau     2880

gugaaagccg gcaacguuag caauuuucgg cacauguucg acaucgugaa ggucagagag     2940

cugaacgauc uucaucacgc gaaagacgcc uaccugaaua uugugguggg gaacgcuuau     3000

uacgucaagu uuaauaagaa cgccgcuugg uucaucaaag agaauccugg acgcucuuau     3060

aaccucaagc ggauguuuga uuacgacauu agacgcaaua acgaaguugc cuggcuggcc     3120

ggcuccaccg ggaccaucag ccaggugaaa aauauggugu cuaagaacca uauucucguc     3180

acacggaaau ccuaugaggu gacgggaggc cuguucgauc agcaacuuau gaagaagggg     3240

aaaggccagg uucccgugaa gaccagcgac gaaagacugg gaaauaucga gaaauacggc     3300

ggguacaaca aggcuucugg agcguauuuc augcucguca aaagcaagaa uaagaaaggc     3360

gaggagcagc gcacaauuga guacgugccu aucuaucuga agcacuacau cgaagagucc     3420

aaagaaaacg cccuggauua ucuuaagaac gagcggaaac ugaaugaacc cgagauucuc     3480

cuggacaaga ucaaaaagga uacccucuuu agaguuaacg gguucuacau gugccugagc     3540

aagcggacag gaaaacagcu ugcuuuuaag ggcgccaaug agcugcuccu gccugaaccc     3600

gagacccugc uucugaaaaa gauuacgaaa uauauccaac gcagaaagga aaacaaggac     3660

cucaaacuga caaagcagga caauauuacc gagaaagagc uccuggaacu uuacgaugug     3720

uucaccgaga agcugaacca cucugucuac gccgugugcc uggggacaca gggcaaaacc     3780

cucguucaga agaagccggc uuuuguggcc cugccugucg aagagaaaug uaucgugcuu     3840

uccgaaaucc ugcaucucuu ccaaugccag agcggauccg cgaaucugaa gcucauuggc     3900

gggcccgcuu cugccggaau ccuguaucuu aacagcaaua uuuccgacgc cggcgagauc     3960

cugauuauca accagucucc cagcgggauc uuugagaaac ggauugaucu gcuccgccug     4020

uga                                                                   4023


<210>  4146
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4146
augaagaaag aguacaccau cggccuggac auugggacaa acagcguggg augguccguc       60

cucacggaug acuauagacu gguuucuaag aaaaugaagg uggccggcaa uaccgaaaag      120

agcuccacaa agaagaacuu cuggggagug cggcuuuuug augaggggca gaccgcugaa      180

gcccgcagau cuaaacggac agcgcgcaga cggcuggcuc gcagacggca acgcauccuc      240

gagcugcaga agauuuucgc ccccgaaauc cuuaaaauug acgagcacuu uuucgccaga      300

cugaaugaga gcuuucucgu cccugaugaa aagaaacagu cccggcaucc gguguucgcu      360

accaucaagc aggagaaguc uuaccaccaa acauauccca ccaucuacca ucugcgccag      420

gcccuggcug acagcuccga aaaagccgau auuagacucg uuuaucuggc gauggcccac      480

cuucugaagu accggggcca uuuucucauc gagggggagc ugaacacgga aaauagcucu      540

gugaccgaga cauuccgcca guuucuuucc accuauaacc agcaauucag cgaagcugga      600

gacaaacaga cagagaagcu ggaugaagcc gucgacugcu cuuuuguguu caccgagaaa      660

auguccaaga cgaaaaaggc ugagacacuc cugaaguacu uuccucacga gaaaagcaau      720

ggcuaccugu cucaguucau uaagcucaug guuggcaacc aggggaauuu caaaaacgug      780

uuuggacugg aggaagaggc caaacuucaa uucuccaaag aaaccuauga ggaggaucug      840

gaagaacucc uggaaaagau cggcgacgau uacauugacc uuuuugucca ggcgaagaau      900

guguaugacg ccgugcugcu cagcgagauc cuguccgauu cuaccaaaaa cacaagagcu      960

aagcugagcg ccgggaugau ucggagauac gacgcucaca aagaagaucu cguccugcuu     1020

aagcgcuucg uuaaagagaa ucugcccaag aaguaucggg ccuuuuucgg agacaacucc     1080

gugaauggcu acgccgggua uaucgaggga caugcuaccc aggaagcguu uuacaaauuc     1140

gugaagaaag agcucacagg caucagaggc agcgaagucu uucugacgaa gauugagcaa     1200

gagaacuucc uucgcaaaca gcggaccuuu gauaacgggg ugaucccuca ccagauucau     1260

cugucugaac ucagagccau cauugcuaau cagaagaagc acuaccccuu ccugaaagag     1320

gaacaagaga agcuggaauc ccuccugaca uuuaaaaucc cuuauuacgu uggaccgcuu     1380

gccaagaaac aggagaacag ccccuucgcc uggcugaucc gcaagucuga ggagaaaauu     1440

aagccuugga aucuccccga aaucguggac auggaaggca gcgcuguccg guucauugag     1500

agaaugauca acaccgauau guauaugccu cacaauaagg ugcugcccaa aaacucccug     1560

cuuuaccaga aguuuucuau uuauaaugaa cucacaaaag uucgcuacca ggacgagcgg     1620

gggcaaauga acuauuucag cuccaucgag aagaaagaaa ucuuucauga gcuguucgaa     1680

aagaauagaa aggugaccaa aaaggaucug caggaguuuc ucuaccugaa auacgacauu     1740

aagcacgccg aacuuagcgg aaucgagaaa gcguucaacg cuucuuauac gacauaccau     1800

gauuuucuga ccauguccga gaauaagcgc gaaaugaagc aguggcucga ggacccggaa     1860

cuggccagca uguucgagga gauuaucaaa acccugacag ucuuugaaga ccgggagaug     1920

auuaagacca gacuuucuca ccaugaagcc acacucggca aacacaucau uaagaaacug     1980

acgaagaagc acuauaccgg guggggacgg cuguccaaag agcucaucca gggcauccgc     2040

gauaagcaaa gcaacaaaac aauucuggac uaccuuauca augaugacga uuucccucau     2100

cacagaaacc ggaauuuuau gcagcugauu aacgacgauu cccucucuuu caagaaagaa     2160

aucaagaaag cucagaugau uaccgacaca gagaaccugg aggaaaucgu gaaggagcug     2220

accggcagcc ccgccaucaa gaaagggauu cuucaguccc ucaagaucgu cgaugaaauu     2280

gugggaauca ugggcuauga gccugcuaau auuguugugg aaauggccag agagaaccaa     2340

acgaccgggc gcggacugaa aucuagccgg cccagacuga aggcgcucga ggaaucccug     2400

aaagacuuug gcagccagcu ucugaaggag uaccccacag auaauucuuc ccuccagaag     2460

gaccgccugu aucuguacua ccuucaaaac gggagagaua uguauaccgg cgccccucuc     2520

gacauccauc ggcugagcga cuacgauauc gaccacauua ucccgcgcuc uuucacaacc     2580

gauaauucca uugacaacaa aguccuggug agcucuaagg aaaauagacu caaaaaggau     2640

gacguuccca gcgagaaagu ggucaagaag augcgguccu ucugguauga ucuguacagc     2700

ucuaaacuua ucuccaagcg caaacuggac aaccucacga agauuaaacu gacagaagag     2760

gauaaggcug gauuuaucaa gagacagcug guggagaccc ggcagaucac aaaacacgug     2820

gccggcauuc uucaucaccg cuucaauaag gcugaagaca ccaacgaucc uaucagaaaa     2880

guucggauua ucacccucaa gagcgcccug gucucccagu uucgcaauag auucgggauu     2940

uauaaagugc gggagaucaa cgaauaccau cacgcgcaug acgccuaucu gaauggagug     3000

gucgcucucg cccugcuuaa gaaguacccc caacuggcuc cugaguuugu guacggcgag     3060

uaucucaaau ucaacgccca caaggccaau aaagcuacag uuaagaaaga auuuuacucu     3120

aacauuauga aguucuuuga gagcgauacg cccgugugug acgaaaacgg ggagaucuuc     3180

ugggacaaau ccaagucuau cgcgcagguc aagaaaguga uuaaucacca ucacaugaac     3240

aucguuaaga aaaccgaaau ucagaaggga ggcuuuagca aagagacagu ggagccgaag     3300

aaggauuccu cuaaacugcu gccuagaaag aauaacuggg accccgccaa auaugggggc     3360

cuuggaagcc cuaaugucgc uuacaccgug gccuucacau augaaaaggg caaagcccgc     3420

aagcggacca acgcucugga ggggaucacg auuauggaaa gagaggccuu cgaacagucc     3480

cccguucucu uucugaagaa uaaaggauac gagcaagcgg agaucgaaau gaagcucccu     3540

aaauaugcuc uguucgagcu ugaaaacggc cgcaagcgga ugguggccag caauaaagag     3600

gcccagaagg cuaacucuuu ucugcucccc gaacaucugg ucacacugcu uuaccacgcc     3660

aagcaguacg augagaucuc ccauaaagag agcuucgacu augugaauga acaccacaag     3720

gaguuuucug aaguguucgc gagaguccug gaguuugcug ggaaauacac ccucgccgag     3780

aagaacauug aaaaacugga gaagaucuau aaagaaaauc agaccgauga ccucgccaag     3840

cuggcuucca gcuucguuaa ccuuaugcaa uuuaaugcca ugggagcucc ggccgauuuc     3900

aaguuuuucg acgugacaau uccucgcaaa cgguacaccu cucugacaga gaucuggcag     3960

uccacgauua uccaucagag caucaccggc cuguaugaaa cacgcauuag aauggggaag     4020

uga                                                                   4023


<210>  4147
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4147
auggagcagg aauacuaucu gggccucgac auggggaccg gaagcguggg cugggccguc       60

acagauuccg aguaccacgu ucugagaaag caugggaaag cucuuugggg agugcggcug      120

uucgaaucug ccagcacggc ggaggaacgc agaauguuuc ggaccucccg cagacggcuc      180

gaccgcagaa acuggcggau cgagauucug caagagaucu ucgcugaaga gauuucuaag      240

aaagaucccg gcuuuuuccu ucgcaugaag gaaagcaaau auuacccuga ggacaagaga      300

gauaucaaug ggaacugccc ggagcugccc uaugcccucu uuguggacga ugacuucaca      360

gauaaggacu accacaaaaa guuuccuacc auuuaucauc ugcggaaaau gcugaugaau      420

acagaagaga cccccgauau ccgccucguc uaccuggcca uccaccauau gaugaagcac      480

agaggacacu uccuucuguc cggcgacauu aacgaaauca aagaguuugg cacaaccuuc      540

ucuaagcucc uggaaaauau uaagaacgag gagcuugauu ggaaucugga acucgggaaa      600

gaggaauacg cugugguuga gagcauccug aaggacaaca ugcugaaucg guccacgaaa      660

aagacccgcc ucauuaaagc ccugaaggcu aaaagcaucu gugaaaaggc cgugcuuaac      720

cugcucgcgg gaggcacagu caagcugucu gacauuuuug ggcuugagga gcugaaugaa      780

accgagagac cuaaaaucuc cuucgccgau aacggauaug acgauuacau cggcgaagug      840

gagaaugaac ucggggagca guuuuauauu aucgagacag cuaaggccgu uuacgacugg      900

gcugugcugg ucgaaauucu gggaaaauau accagcaucu cugaggccaa gguggcgacg      960

uacgaaaaac auaaguccga ucuccaguuc cugaagaaaa uugugcggaa guaucuuaca     1020

aaagaggagu acaaggacau cuucgucagc accucugaua aacugaagaa cuacuccgcc     1080

uauaucggca ugacaaagau uaauggcaaa aagguugacc uccagagcaa aagaugcucc     1140

aaggaagagu uuuacgauuu caucaaaaag aacgugcuga agaaacuuga agggcaaccc     1200

gaguaugaau accugaagga ggagcucgaa cgcgagaccu uucugccuaa acaggugaac     1260

cgggacaaug gagucauucc guaucagauc caccuguacg aacucaagaa aauucugggc     1320

aaccuuagag auaagaucga ccugaucaaa gagaaugaag acaagcucgu gcagcuguuc     1380

gaguuucgca uucccuauua cguugggccu cugaacaaga ucgaugacgg aaaagagggc     1440

aaguucaccu gggcugugcg gaaaucuaau gaaaagauuu accccuggaa cuuugagaau     1500

gucguggaua ucgaagccag cgcugagaaa uucauuagac gcaugacaaa caaguguacg     1560

uaucuuaugg gggaagacgu ucucccuaag gauucccugc uguacagcaa auauauggug     1620

cucaaugagc ugaacaaugu caaacuugac ggagagaaac ugucugugga acucaagcaa     1680

cggcuguaca ccgaugucuu uugcaaauau agaaagguga caguuaagaa aaucaagaac     1740

uaccugaaau gugagggcau uaucuccggc aauguggaaa ucaccgggau ugacggagau     1800

uucaaggcca gccuuacagc cuaucaugac uuuaaagaga uccucaccgg cacggagcug     1860

gcuaagaagg auaaagaaaa cauuaucaca aauauugucc uguucgggga cgauaagaaa     1920

cuccugaaga aacgccuuaa ccggcuguac ccccagauca ccccgaacca gcucaagaaa     1980

aucugcgcgc ugucuuacac cggauggggc agauuuagca agaaguuccu ggaggaaauu     2040

acagccccug accccgagac cggggaagug uggaauauca uuacagcucu uugggagucc     2100

aacaauaacc ucaugcagcu gcugucuaau gaguaucgcu ucauggaaga gguugaaacg     2160

uacaacaugg gcaaacaaac caagacacuc agcuaugaga ccguggaaaa uauguacguc     2220

uccccuagcg ugaaacggca gaucuggcag acacugaaga uugugaaaga gcuugagaag     2280

guuaugaagg aaucucccaa aagagucuuu aucgagaugg cccgggaaaa gcaagagucc     2340

aaacgcaccg aaagcagaaa gaaacagcug aucgaccucu auaaggccug uaagaacgag     2400

gagaaagauu gggugaagga acugggagac caggaggaac agaaacugcg gucugauaaa     2460

cuuuaccucu auuacacgca aaagggcaga ugcauguacu ccggggaggu gauugagcug     2520

aaggaccugu gggauaauac caaguaugac aucgaucaca uuuaccccca gagcaaaaca     2580

auggacgauu cccucaacaa ucgcguccug gugaagaaaa aguauaacgc uaucaaaucu     2640

gacaaguacc cucuuaauga aaacauucgg cacgagagaa agggauucug gaaaagccug     2700

cucgauggcg gguuuaucuc caaggaaaaa uaugagcgcc ugaucagaaa uaccgaacug     2760

ucuccggagg agcuugccgg auucauugaa cggcagaucg uugagacacg ccagagcacc     2820

aaggcggugg cugaaauucu gaaacaaguc uuucccgagu ccgaaaucgu guacguuaag     2880

gccggcacgg ugagcagauu ccggaaagac uuugagcucc ugaagguccg cgaggugaac     2940

gaucuccauc acgccaagga cgcuuaucug aacauuguug ugggcaauuc uuacuacguc     3000

aaauucacaa agaacgccuc cugguuuauc aaagaaaauc cugggagaac cuauaaccuu     3060

aagaaaaugu ucacaagcgg auggaauauu gagcggaacg gcgaaguggc uugggaggug     3120

gggaagaagg gaaccaucgu caccguuaaa cagaucauga auaagaacaa uauucuggug     3180

acacgccagg ugcaugaagc caaaggcggg cucuuugacc agcaaaucau gaagaaagga     3240

aagggccaga uugcgaucaa ggagacggau gagagacugg ccucuauuga aaaauacggg     3300

ggcuauaaca aggcugccgg agcuuacuuc augcuggucg aguccaaaga caagaaaggc     3360

aagaccaucc ggacaaucga auuuauuccc cuuuaucuga agaauaaaau cgagagcgau     3420

gagucuauug cccucaacuu ccuggaaaag gggcgcggac ucaaagagcc uaagauccug     3480

cuuaaaaaga uuaaaaucga cacccuguuc gauguggacg gcuuuaagau guggcucagc     3540

gggagaacag gagaucggcu gcuguucaag ugugcgaauc agcuuauccu ggacgaaaag     3600

auuaucguua ccaugaagaa aauugugaag uuuauccaga gacgccaaga gaaccgggaa     3660

cucaaacugu ccgauaagga cggcauugau aaugaggucc ucauggagau cuacaacacg     3720

uucguggaca agcuggaaaa cacaguuuau cgcauuagac uuagcgagca ggccaaaacc     3780

cugaucgacc ggcagaagga auuugagaga cugucucucg aagauaaauc cagcacccug     3840

uucgagaucc uucacauuuu ucaaugccag uccucugcug ccaaucugaa gaugaucggg     3900

ggccccggaa aagcuggcau ucucgugaug aacaauaaca ucagcaagug uaauaagauu     3960

uccaucauca accagucucc gacagggauu uucgagaaug aaaucgaccu gcucaaaauu     4020

uga                                                                   4023


<210>  4148
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4148
augagauucc agaacaagaa aaccggcgac uacuuugugg ggcuggauau cggaacaaau       60

agcgucggcu gggccguuac gaacgagaag uaugaaauuc uccgggcuaa aaaucaccgc      120

augugggggu ccagacuguu cgaggaagga aagaccgccg aggaacggcg cgugcauaga      180

gcgggccggc gcagacuuaa ccggcgcaau gacagacuga ugauccucga ggaucuguuu      240

gcugaggaaa uuucuaaagu ggacaaggcc uucuaccaac ggcuucacga gagccaguau      300

cgccaggaac auaagacagu cuccgggccc uacuuucugu ucaacgauaa agaguuuacc      360

gacgaggauu aucacaucga cuacccuaca auuuaucauc ucagaguggc ccugaugaag      420

gcuccggcca aagauguucg gcuccuguuc cuggcuaucc accacaucau uaaguaccgc      480

ggaaauuuuc uucaugaagg cgagaccuuc aaaacaaccg gcgggcugac ggacauccuc      540

cagagagugc uggccugcaa cgaauuuucu ggacuuaagc aacacauuga ugacaccgcg      600

cuggucaagc uccugacaaa uaaaggcauc acccggagcg auauugccaa gcagaucucc      660

gagcuggcua cagacaaacu cuacagaaag caggccaagg cuuucgccuc ucuggugcuu      720

gggcugaagg cgaaccucga cgaucuguuu aaugaacccg aguauaaaga gcuugacagc      780

gaaauucgca ccguugaguu cuccggaaag gaauacuggg aggaacggga uaaguaugag      840

ccuuaccugg gcgagagccu cuucgcccug gacgggugua aaacgcugua ugauacacuc      900

cugcuuucua acaucaagcc cgacggaaaa uaccuguccg aagcuaaggu ggagaccuuu      960

gaaaaacauc acgagcaccu cgaaagccug aaggaucuuc ugaagucuaa uaaagcccuc     1020

uauaacagag uccugcgcga cgaugagaag aaucugaaca acuacaucac auacauuggc     1080

aaauccgagu ucaauaagcg gugcagcaga gacgaauuuu auaaauuccu caagcagacc     1140

aucaaggaua uuucugacaa cgagaauaaa gcugaaaucg ugcaacugau ggagcuugac     1200

uccuuucugc cucuccugcg cgugcgggag aacggcguca uucccuacca gauccaugaa     1260

gaggaacugg agcagauccu uaauguuagc uccgaaaagu auccuuuccu caacaaagcc     1320

gaugggucug gauuuaccau ugcccagaag auccugaaac ugcucacauu ccgcauuccg     1380

uacuuugugg gcccccugaa uccugcucac aaggacagag auaacggguu cgcgugggug     1440

guccggaagg cccccggaaa aguguauccu uggaauuuug aggacaaggu ugauaucagc     1500

gcuuccgcca cggccuucau ugagaaccuu accaauaaau guacauaccu gaucggcgaa     1560

gacgugcucc ccaaguauag ccuggcuuac ucuaaauuuu cccugcuuaa cgagcucaau     1620

aaccugagau acgaugggaa gccgcugagc uaugaaacca aacaaucuuu caucaaggac     1680

ugcuucgaga aggaacaucg caaaaugaca gagaagcgga uuagagaguu ucucaaacac     1740

cugggaggca ccgauggcaa gggggcccuu acgggacugg acgucgauau caaaagcgac     1800

cucaaguccu accgggauau ggugcgcauu cugggcgacg gguucgacga aacaauggcg     1860

gagagaauca uucgguaugu uacccuguuu ggagaagcua agaaagugcu ucucucuacc     1920

cuggagaucg aguucggcga ugaccugagc gauacacaga agaaacagau ucucaagcug     1980

caauacagag acugggggcg ccuuuccgcc acccugcuca aaggcaucaa gggagucgcc     2040

ggcgauaauc cugaauguag cauccuggag ugcauggaac agcagccccu gaaccuuaug     2100

gaggugcucu cuucccauaa cacauaucug gcugccauug aagaguuuaa uaaggagcgg     2160

acggaaccua ccgacaucgu cgauuacaaa cugguggacg aucucuauau uagccccgcu     2220

guuaagagau cuguguggca gacacugcgc auccuugacg agauuaccaa acugagaggg     2280

uccgucccca agaaaguguu cguugaagug gcccggacaa accgcgcgga gaagaagaaa     2340

accgauagca gaaaggaacg gcuccugcaa cuguacaaaa cgaucaagga cgagggacgc     2400

aauuggaccg aggaaaucaa acagagagau gccggcgacu uuaacuccaa gaaacuuuuc     2460

cuguacuaua cacagcaggg gcgguguaug uacucuggag agaagauuac cagcaucggc     2520

gaccucuuua cauccaagua ugauauugac cacaucuacc cucgcucugu caccaaagau     2580

aauagcuggg acaaccuggu gcucgugaag gcugaacuga auagagagaa aaaggauauu     2640

uauccgaucg aaaaagacac gcaagaucgg ugcaucuccc uuuggaagca gcugcucgag     2700

caggggcuga uuagcgagcg caaguacgaa agacugguuc ggacaacacc ccuuaccgcc     2760

gacgagcugg cugauuucgu caacagacag cucguggaaa ccaaucaauc ugugaaagcc     2820

gucaccaacc ugcucaagcg ccuguauccu gacacaacga ucuguuacgu gaaagcggag     2880

aauguuuccg auuuucggca ggaaagagag uucgugaaac uucgcagccu gaacgcccac     2940

caucacgcua aagacgccua ccucaauauu gucgugggca acguuuauca ugagaaguuu     3000

accaauaacc cccggaacuu caucaagaau gcuagaucug acaaaaagcg cccuuacucc     3060

cugaaccaca uguucgauua ucugcuucag gugggaaaua aagaaauuug gaaccccaag     3120

acagacaaua accuggucaa aaagaugaug cggaauaacg augugcaggu uaccagaaug     3180

cucguggagc aaacaggcgc ccuguacgac gccaccaucu auaaggcuag cgucacgaaa     3240

ucugaaagcu acgugccgcu caaguccagc gaugcgaucc ugucugacgu gaaaaaguau     3300

gggggauuua cauccauuaa aaucgccuac uacagcauuu uccgcuuuua uaagaaaaau     3360

uccgaggagu ucgaaaccca caucauuucu aucccugucc uuaaagcugg cacccugaac     3420

agcaaggagg auuacguugc cuauggggaa gagauuauga auaaacagaa guuugaccgg     3480

guggaacugg uguacaaaaa gcucugcaug aacucccugg ucagaaucaa uggagugaug     3540

uauuaccuug gcgggaaaac aaacaauucu cuguauaucg auagcgccau uucccucguu     3600

cugcccgaca acaucgcuaa gaagauuaaa gccguggaga aguacaucgc gcgggucgcu     3660

gagaacggca auuacaaacc ucagcccgau caagugucuc cugaauuuaa caugauuauc     3720

uauaaggccc ucaccgagaa aaugaagcag cccgccuucg uuggacugaa gacaaaucgc     3780

gaaaacgagc uugaaaaaga gacgaccauc acagcuuuca uugccaugac ccugaucgag     3840

caguguaaga ugcugaugga aauucucaau cugcuuacac agcaaaagac cacguacgcg     3900

cugaagccga ucggcgugag cgcuuccaga agcacccucg ggaugaacau uacaggacug     3960

ucugaguuua ccaucaucac acaguccguc accggccucc aggaaaaaga gauucggauc     4020

auuuga                                                                4026


<210>  4149
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4149
auguggggca gcagacuguu cgaggaagcc uccaccgcuc aggaccggcg cgagcacaga       60

ucuaaccggc gcagacucga acggcgcaag uacagacugg aucuucugga ggaacucuuu      120

guggaggaga ucaauaaaau ugaccccgag uucuuuaucc ggcugcgcga gagcaaguuc      180

cauuuugaag auagaucccu uaaaaacaca uucauucugu uuaaugacaa gauggaaggg      240

ggauauaacg auaaagucuu cuuuucuaag uacccuacga ucuaucaccu ccggagcgcc      300

cugauuaaug gcaacaagga ggacguucgc gcgguguucc uggcuaucca uaauauccuc      360

aaaaacagag ggcacuuucu gcuugccgga aaugauauua acaucucccu gucucucaau      420

gacacccuga cagaucuuuu cgaauaccug aagauucauc ucggcaucga gguggaauuu      480

gagaacaaac ugaaggacau ugaaaagauc gcccuggaua agaaucucag caaguccgac      540

aaacaaaaga aaauugucca gcugcuugag accgauuaua agaaacagcu ggcugacgug      600

uucaagcuca gccuggggcu uacagccaac auggagaaua ucuucggaaa agaaaacgag      660

cagggccugg aauuuaucaa cgcugagaau aagauuaaga aaauccaauu cgacucuucc      720

uacgaagaga acauugccga guuugaucag uucuauggcg aauacuuuga gaucauugac      780

aucugcaaga aaaucuacga ugcgauuagc cucucucaga uccugguuga cgggaaggga      840

cuguccgaaa gcaaagugcu cucuuaugag aagcacaagg aagaucugaa acuucugaag      900

aaaguccuca agaauauuga caaagccuac gauaacaagg gcgaggaccg gcugguguau      960

aagguucuua aagaggauaa ggacaaaggg accaauuacg ugaacuauuc caagcugagc     1020

aauaacggaa cagcuaccac gaaagaagag uucuacaagu uucucuccaa gaaucuggau     1080

cugaaaaagu ucggcaaacu caacaaggaa gacaucgaga cccuggacua uauuaauucu     1140

gagauccuuc uggggcgcuu ucucccgcug cagaaagaaa agaucaacgg agucauuccc     1200

uaccaacugc agaugaaaga gcuuacacag auccucaaua acgccagcaa guaccugccu     1260

uuccugaaua agauugaaga uggcaugacc gugaaagaca agaucauuuc ccucaucaaa     1320

uuucggauuc ccuauuacgu gggcccucug aacgaguaua acagaaucga aaaugggaac     1380

ggauucgcuu ggaucgagaa gaaaaagaau gagaagauuu uucccuggaa cuucaaugau     1440

gucguugaca ucgaaaagag cgccgagaag uucauuaaca aucuuacaaa caaauguacc     1500

uacaucaagg aagagacggu gcugccuaaa aauucuauua ucuauuccaa guuuauggug     1560

cucaacgaac ugaauaaccu gcaguacggc aauaagaaac uugauaucga gcucaagaug     1620

aaaauguaua cagaccuguu ccuggagaag aaagauaaca gaaaggucac cgaaaagcgc     1680

auucucgagu acuuucggau caaugggauu aacgacggaa aagaaaagcu gacaggccuu     1740

aacaaugaga uccacgcgga uaugaaaagc uacagagaca ugugcgaaau uaugggggag     1800

aacuucaaua ucaacguggc cgagaauauc auuaacugga ucaccguuuu ucauggagaa     1860

aagaaaauuc ugaucaauaa gauuaaaaac guguauggca agauguucaa ugaugagcaa     1920

aucucuaagc ucagcaaacu gaacuacucc gacuggggcc gccugucuaa gaaacuucuc     1980

accgauauca aguguuauau ugacgcugac gggaucaauu uuccggucag cauucuggaa     2040

gccauggagg uguccaacaa aaaucugaug gagcuccuga gcaacaagua cgaauuuauc     2100

ucuugcauug augacaucaa uaacaaguau uuuggagauc gggacauuuc cgcuagcgag     2160

cuuaucgaug aacuguacgu uucucccgcc gugaaaagag gcaucuggca guccguccgc     2220

auucucgagg aaaucgugag cauuaucggg cacaacccua agaaaauuuu caucgagaug     2280

acacggcaua augaggaaaa gguccagaaa uccucuagac gccacaaccu ggaggaacug     2340

uauaaggcca uccggaagga ccccgaguac guggaacuua uuaaagaguu ugauagcacg     2400

accgacacag auuccuucaa gaucaaaaag cucuaccugu auuuuaugca acugggaaaa     2460

ugugcuuacu cuaaggagaa gauugacuuc gauaaacucu ucaccgacag cuaugauauc     2520

gaccacauuu acccuagauc caagacaaaa gacgauagca uccauaauaa ccugguucuu     2580

gugaaggcga aauauaaucg cgaaaagacc gacgucuacc ccaucgauau uaacauccag     2640

aagaauauga acucuuuuug gaauuuccug uauacgaaaa aguuuauuac agaggaaaaa     2700

uacaagcggc ucaucagaac cuccgaccug accgaugagg aacuggccaa auucauuagc     2760

cggcagcuug uggagacauc ucaguccacc aaggcuacag ccgagguucu gaaacaaauc     2820

uacggcgaca gcgauauuug cuaugugaag gccgaaaacg ucucugacuu ucgcuacggg     2880

agcuccagac agaaggauaa aaaugacauc cagaaacucu ucgugaaaug ucgggaggug     2940

aaccacuuuc aucacgcuaa ggaugccuau cugaauaucg uugucggcaa cguguacgac     3000

gugaaauuca cgaagaaucc ggucaacuuu auuaagaaaa auaagauggg aaacaaaagc     3060

aacuauucuc ucaaucauau guucugcuuu gacguggaaa gaaacggcua caaggcgugg     3120

gauuccagcg agaccaucaa aacaguuggg cgcaugauga aguccaauga cgugcggauu     3180

accaagcacg uccacccuac aagaggagag cuguaugaug cuaccaucua caaagccaag     3240

auugccaaaa cgggcucuua caugccccuu aagagcucca aaaacacccc ucugucugac     3300

gugacaaagu augggggaua caccaagauc aaaaucgcuu auuacagcgu uuauaaguac     3360

aaauucuccg auaagaaucu cauuaaaaca gaaauccgcc ugauucccau ccccauuuau     3420

cugucuaaga acgacgauga caucaaagag uacgugaaga auuaccuuaa cgauagaguc     3480

aauagcaagc ugaucgacuu caccaaaauu uaugauaagc ucuacgugaa cucccugauc     3540

aaaauuaaug gcuaucucua cuaucuuggg ggcaagacga acgaccgggu uuggaucgau     3600

aaugccguga gcauuguccu ggacgacaaa aucgaauacc ugauuaagcg caucucuaac     3660

gauaucaagu auggauacga gauuaaugug uccgacgaug aacucaugga caucuacgag     3720

agccuggcug agaaagccaa caagaaaauu uucuauaaca gacugucuga uuucgaaaag     3780

aucuuuucca auauggacgu gaaaaagaag uucgucaaac uugagaagaa cgauaaaugu     3840

cggguucugc uccagcugcu caaugugcug acagacagcc aaaccacaua caaacuugaa     3900

auggugggcc ugaaacauuc ucgcuccaac auugggauga agaucagcaa ucuggaggaa     3960

uuuaagguca ucaacuccag cauuaccgga cucuauucua augagaucga uauucugaac     4020

aucuga                                                                4026


<210>  4150
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4150
auggagaagg acuacuaucu gggccucgau auggggagcg gauccgccgg cugggcugug       60

accgacgauc aguaccacgu ccugagagcc caugggaaag cgaugugggg aacacggcuu      120

uucgaaucug cuaacacggc cgaggaacgc agaaucaauc ggaccgcccg cagacggcug      180

aagcgcagaa accaacggau ugaguggcuc caggaaaucu uugcugagga gauuaauaca      240

aaagacagcg gcuucuuucg cagaaugaag gaaucccggu auugggccga ggauaaacgc      300

gacgaaaacg ggucuugccc cgagaugccu uacagccugu ucaucgauga ggacuuuacc      360

gauaaggaau aucacgagaa guucccgaca auuuaccauc uuagaaaaua ucugaugacc      420

acagaagaga cccccggaau ccggcucguu uaccuggcuu uucaccauau gaugaagcac      480

cgcggccacu uccugcucaa uggcaacauc gaagagauua gaaaauuucg gaagacguuc      540

gaccaguuuc uggagguggu gagaaaugaa gagauggggu ucgccggaga aaucgaggaa      600

gagaccuaca aaaagauuga gaugacacuu aaggauguca accugacccg cuccgcgaaa      660

aagacaaccc ucaucaaauc ucugaaggug acgcggguug aagagaaaga acuucuggcc      720

cucauuagcg gcuguacagu gaagcugucc aauaucuuua acgacaaaag ccuggaugag      780

accgaaagac cuaagauuuc uuucgcugac gccggguucg acgauuauga ggcugagauc      840

caguccaauc ucaacgaaaa guacuauauc auugagagcg ccaaagcggu cuacgacugg      900

gccgcucugg ccgauauccu uggagaauau ucuugcauuu ccgaggcuaa gguggcccug      960

uacgagaaac aucacaagga ccucaaacgc cugaaggauc uuguucggaa uaacaucagc     1020

ucugaagugu auaaggagau uuuugucgac guguccggca aauacaagaa uuacugugcc     1080

uauaucggga caaccaacau caacggaaag aagaaagauu ucgaaggcaa gaagugcacc     1140

aaagaggacu uuuacgcuua ucugaagaaa agcgugauug aauguaucaa ugauuccgag     1200

aagacagacu ggcucaaacu ggagcuggaa aagggcaacu uccuccccaa gcaacugacg     1260

ggggaaaauu cugucauucc uuaccagguu caucuuuaug agcugaacgc gaucauuacc     1320

aaucuccagg agagaauccc ccugcuuaaa cgcgauggag aaaagauccg gcagauuuuu     1380

acauucagag ugccuuacua ugugggcccg cugaacggga ucagcauuga cggcaaaaag     1440

accaauuggg ucaaacgcaa ggagggagug aucuaccccu ggaacuuuuc cgacauuguu     1500

gaugaggaaa gcucugccga guccuucauc cggagaauga caaauaaaug caccuaccuc     1560

auucacgaag acgugcugcc uaaguauagc cugcucuacu cucgcuuuga gguccugaac     1620

gaacuuaaua acgugcggcu gaauggggag cuccugagcg uugagcugaa gcagaaaauc     1680

uaugaagaug uguuccagag auaccggaag gucacgcaga aacgccuuaa gagauaucuc     1740

cugacagagg gaaucauuga cgaaaacacc gugaucaccg gccuggaugg cgacuuuaaa     1800

gggucccuca cagcuuacca cgauuucaag gagaagcuga ccggacuuaa ucugacagag     1860

cgggaaaaag aggaaauuau ccucaacauu acgcuguuug gcgacgccag aucucugcuu     1920

cgcaagcgga ucgggaaaau cuguagccag cucuccgaua agcaaaaaga gaaccugagc     1980

aagcugucuu aucagggaug gggcagacuc uccaagaaau uccugaaugg gauugccauc     2040

agcguccagg gcgaagagga guggaccauu aucaaggcuc uuugggaaac acaagacaac     2100

cugucucagc ucaugggagc caaauacacc uuccgcaaug cgguggagaa ggaaaacggc     2160

gagcuggaaa auagagagau uuccuacgag acaguuaaag aucugagcau cucccccucu     2220

gugaagcggc aggucuggca gagccuucuc aucgugaagg aaauuugcaa aauccaaaag     2280

aaaccucccc gcaagguuuu uguggagguc gcuaaagaaa agaccaacuc cgggagaacg     2340

gagucucgga aaaagcgccu gcaggacuuc uauaaguacu guagcaaaua ugaaaaggag     2400

cuguacgaug aguccauuca gaauaaacuc gaaaagugca gcgagucuga ccugagaucc     2460

gacaaacuuu uucuguauua caugcagaug ggacggugua uguauaccgg ccaaccgauc     2520

gauuucgacg aacucuggaa caaucagaag uacgauauug accauaucua cccucagagc     2580

aagacaaugg augacucuau cgauaaccgc gugcugguga aaagaauuuc caaucaggag     2640

aagaccgacg uuuaucccau ccgggccgau auucgcgaga aaagacgggg guuuuggaag     2700

gaacugaaaa acgucggacu uaucagcgag gaaaaguaca agagacucac acgcgccacc     2760

gaguucgaac cugcugagcu ggccggcuuu auuucucggc aacuggugga gaauagacag     2820

acgacaaaag uggucgcuag cauccucaag caggccaugc ccgacaccga aauuguguau     2880

guuaaagcgc gcacaguguc cgaguuccgg cacaaguuua acuucaucaa agucagagaa     2940

augaaugauu uucaucacgc caaggacgcu uaccugaacg ugguuguggg gaacaccuau     3000

cuugucaagu ucaccaaaag ccccgugaau uuuaucgaga accauucuaa ucgcuccuac     3060

aaccugaaga aaauguucac agauggccgg guugaaagaa auggagagau ugccuggauc     3120

agcggcgaga acgggacgau uggaaccgug cgcaauauca ugaagaaaaa caauauucuc     3180

gucacacgga gagcucacga agccaccggc ggguucuuug accagcaacu gaugaagaag     3240

ggaaaaggcc aggugccugu gaaggggucc gacgagcggc ugcuugauau cgaaaaauau     3300

ggcggauaca acaaggagac aggcgcguac uucguccugg uugaaucuga cgagaaaaag     3360

gggaaaaaga agcgcaccau cgaguaugug ccgauuagac ucaaaaagca gcuggaaaau     3420

aaccggaaag cccucgagaa uuaccugugc gaacaacgcg gacuuacgaa ccccgugguc     3480

cugaucgaua agauuaaaau ggacagccuc uuuagagugg auggcuucuu uauguggcug     3540

uccgggcgga caggaaauca gcugcuuuuc aagugcgcua accagcuggu ucucucuacc     3600

gaggacgagg ccacccugaa gaaaaucgug aaguuuguca acagacgcaa agaaaauaag     3660

gagauuaaaa ucuaugaaag cgauaagcuc aacgaggaaa ugcugaaucg gcuuuacaac     3720

acauuccugu auaagcucca aaauuccauc uacggcaaca gacuggggga gcaagcuaaa     3780

acccugucua gcaagaaaga gaauuuugac aaacuucaga uugaagagaa augucuggug     3840

cucuaugaaa uccugcacau guuccagugc caguccgcca caagcgaucu ccaacugauu     3900

gagggcccug gaaaggccgg cacgcuucgc cugaacucua auaucaccaa guacaaaaac     3960

auuaauauca uuaaccaguc ccccacaggg aucuacgaac agguuaucga ccugcagaag     4020

auuuga                                                                4026


<210>  4151
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4151
augggcaagg gguacuaugu gggacuggac augggcaccg ggagcgucgg augggccguu       60

acagaugagu ccuaccagau ccucagacgg cacggcaaag cuaugugggg agugcgccug      120

uucgaaucug ccaagacggc ggaggaaaga cggauguuuc gcaccggaag acggcgccuu      180

gacagacggg gcuggcgcau ugagauccug caagaaauuu ucgcugagga gaucagcaga      240

guggaucccg gcuuuuuccu ccggaugaaa gaauccaagu auuacccuga ggacaaacgc      300

gauauucagg ggaacugccc ggaacugccc uauacacuuu uugucgacaa gaccuucaca      360

gauaaggacu uucauaaaaa guacccuacc aucuaucacc ugagaaaaau gcucauggag      420

acagaggaua cccccgacau gcggcuggug uaccuggccc uccaucacau gaugaagcau      480

cgcggacacu uccugcuuuc uggcgauauc agccagguua cggaauuuaa aaauaccuuc      540

aaccaguuua uugagaauau cagaaacgaa gagauggacu ucgaaauuga gcuggaugag      600

uccgccgugc aaaugaucga agagacacuc aaggacaaga aucugacccg gucugcuaaa      660

aagagcaaac uugucaaggg gcugaacgcc aaaaauguga gagacaaggc uuuucucaca      720

cugcuguccg gaggcaccgu uaaacucagc gaucuguucg ggauggaaga acuuaacgaa      780

ggagagcgcc cuaagauuuc uuucgccgac aauggcuacg aggaauaugc ggccgugguc      840

gagauggaac ugggcgagcu cuacuauauc guggaauccg cuaaggccgu guacgauugg      900

gcuauucuga gcgacauccu ugggggaucu acguccguca gcgaugccaa aguucgggcg      960

uaugagaagc acaaagccga ccugaaguac cucaaagcug uggugaagga guauuuuccc     1020

aaggaagucu acaacgccgu guucguugag ucuuccgaua aacugaauaa cuacccugcu     1080

uauaucggca ugacaaagaa aaaugggaag aaagugagcc uggaaggaaa gagauguucc     1140

cgcgaggagu uuauggacuu ccucaagaaa aacauugucg ugaagcugcc ggaugaagag     1200

gccaaaaugu accuucaguc ugaacuggag aaggacagcu uucuccccaa acagguuaau     1260

aaggauaacg gcgugauccc uuaucagguc cauaaguacg aacugaaaaa gauucuugac     1320

aaccuggggg acaaaauccc cuuccucaag gagaaugccg agaaaauuga aaagcuguuu     1380

uccuuccgga ucccuuauua cgugggaccc cugagaaccg gcaacggcga gaauagcaaa     1440

uuugcuuggg cggaacgcaa gucuacagag aagaucuauc cguggaacuu cgaaaauguc     1500

auugaugugg agcaauccgc cgagaacuuu auccggagaa ugaccaauaa augcaccuac     1560

cucauugggg aagacguucu gccuaaggau agccuucugu acucuaaauu cauggugcuc     1620

aacgagcuga auaaccugcg ccuugacgga cagaagauca gcgucgaacu caaacagaag     1680

auuuaucggg auguguuuug uagaucccgc aagguuacac agaaaaagcu gaaaucuuac     1740

cugauccggg agggcaucgc ugggaagaau guggaacuca cgggaauuga cggcgauuuc     1800

aaagggagcc ugaccgccua ucacgacuuc aaggagaaac uuaucgaugu cgagcugaca     1860

caaaaggaaa aggaggaaau uauccucaac auugugcugu uuggcgacga uaaaaagcug     1920

cuuaaacaga gacucaagaa gaaauucccc cagcugaccg agaagcaaau caauacagug     1980

accgcccugu ccuacaaagg auggggccgg cucagcaaga aacugcuuga ggaaguuacg     2040

gucccugcuc ccgagacagg ggaagugugg aacauuauca ccgcccugug ggagaccaau     2100

gacaacuuua ugcagcuccu gucuaaggaa uaucaguucu ccgaucgcau cgaggaguuu     2160

aacgaagaga aagcggaccc cgaagugagc uaccagacaa uugaggaccu guaugucucu     2220

ccugcuguga agagacaaau cuggcagacc cuucagauua uccaggaaau ugagaagguu     2280

augggagaga aaccgaagcg gguguucguc gaaauggcca gagagaaaca agaaaauaag     2340

cgcacagagu cccggaaaaa gacgcucagc gaucuguaca ccaagugcaa aaaggaagaa     2400

cccgagugga ucgaagcccu guguacaucc cucgagaaac augaggagca ccagcugaga     2460

ucugacaaac uuuaccugua uuacacccag aagggccgcu gcauguauag cggggagccu     2520

aucgaucucg aagaccugug ggauaacaca aaguacgaca uugaucacau cuauccccag     2580

uccaagacca uggacgauuc ucugaaaaau agagugcuug uuaagaaaga guacaacgcu     2640

aagaaaagcg acacguaucc uauugccgcu gauauccaaa agaagaugau gcccuuuugg     2700

aaaucccucc ugaccggagg cuucauuccg aaggaaaaau acgaccggcu ggugcgcaau     2760

aacccucucg augccaauga gcuggcgggg uuuaucgaaa gacagaucgu cgagacacgg     2820

cagagcacca aggccguggc ugagauucuu aaaaagaucc ugcccgacac agaaauuguu     2880

uacgugaagg ccaaaaccgu cucuaaguuc cgccaggacu uugauuucau caaagugaga     2940

gacaugaacg aucuccauca cgcuaaggac gccuaucuga auauuguggu cggcaacguu     3000

uacuuuguga aauucacgaa gaaugcggcc ugguucguga aagagaaccc uggacggaca     3060

uauaaucugg aaaagauguu uaucugggau gucgcucgcg acggcgagau cgccuggaag     3120

gcugggaaau ccggaagcau ugugcaaguu agagccauga uggaaaagaa ccauauccuu     3180

gugacccggc gcucuuacga ggucaaaggc gggcuguucg aucagcagau ucucaagaaa     3240

ggaaagggcc aagugcccgu uaaggagucc gacgaaagac ugcgggauau cgggaaauau     3300

ggcggauaca auaaggccgc uggcguguau uuuaugcucg ucagcucuaa agacaagaaa     3360

gggaaggagc agagaacaau ugaguucgug ccuaucuacc ugaaggaucg cguugagaaa     3420

gacauggaaa gcgcgcggaa cuaccuuaug gaggagagag gacugcgcga acccaagauu     3480

cucaucaaaa agaucaaaau ugacacccug uuuaaggugg auggcuucua uauguggcug     3540

uccgggcgga ccagcaacca gcuuaucuuu aagggagcca aucagcugau ucucucuaaa     3600

gaggacauga agacacugaa aaaggucgug aaauacaucc aaagacagaa ggaagauaaa     3660

aacgugaaga uuuccaugcg cgacggcauc acggaggagg aacuccugaa acuuuaugag     3720

accuuccugg auaagauccg gcacagcauu uacggggcua gacucuccuc ucaggaaaag     3780

acacugguca auggcaaaga gaaguuuauc gaccugagcg uugaagccaa augucaggug     3840

cuuuaugaga uucugcaucu cuuccaaugc caguccggag ccgcuaaccu gaagaugauc     3900

ggcgggccgg ccucugcggg gauucucgug augaauaaca auaucagcaa guguaaaaac     3960

aucuccauug ucaaucaguc uccuaccggc aucuacgaga aggaaauuga ucugcuucgc     4020

guguga                                                                4026


<210>  4152
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4152
augacccaga aggugauccc cuacaacauu ggccuggaca ucgggacaaa uagcgucgga       60

ugggccguua cggauaacga gaauaaacuc cugcacauua agggccauaa cgggaucgga      120

gugagacuuu ucaaagaagg ccaauccgcu gccgagcggu gcggguuucg caccacaaga      180

cggcgccugu cuagacggaa guggcgccuc agacugcuua augaaauuuu cgcggacaaa      240

aucgcuaagg uggauccuag cuuuuucgcc cggcugaagc aguccaacgu cucuccgaaa      300

gaccccaaua agaccauguu uggaaacauc cucuucgaua auggcaaccu gaaugacaaa      360

uccuuucacg augaguauaa gacaauuuac caucugcgcc aguccaucau ugaccacccu      420

gaaaagaagu ucgauaucag acucauuuau cuggccaugc aucacaucau uaaguaccgg      480

ggccacuuuc uuaaccaggc uaaugugcaa gacuucaaag ggggaaagau cgaucuggag      540

gagucuuuua aagaccucaa cagcaucuuc gaaaaucagg gccgcgaucu gagacuuguu      600

accgaggacg ccaacaagua ugugaaagaa cuguccgaca acagcaagac acggaaugau      660

cgccagaaag agcucucuaa gguccuguac auucccaucg acaagaacuc cgauaaaaau      720

aacaagaaag ugaccacgga gauuaucaag gcuauucugg ggaugaaagc caaguuugac      780

guuaucuucg gaaucgaugc ggaaaaugcc aaggagugga gccucaccuu caacucugac      840

gauuuugacg auaaaauuga aggccugucc gcucagauga cagacgaggc cggggaaauc      900

cuucugauuc ucaaggagcu guauuucgcu cuuaaucuga gcgauauccu caacaauucu      960

gugaccgaca aaaacgccaa gacacugucc gaggcgauga uuaauagaua cgacgaucau     1020

aaagaacaac ugaccaugcu caagcagguc aucgacaaca gcgauaagaa acacgccaag     1080

gcucugaaag ccgcuuacuc cgccuauauc gacggagaga auaacaagaa aauuucuuuu     1140

gaagaucuuc ggaagagaau ccagaagaau cuggacaaca gcgagacguc caaaaagauu     1200

aacgaucucc uggaacauga cuacuuccuu ccuaaacagc gcagcaagga gaauggcgcc     1260

aucccccacc aacugcagca gcaagagcuc gauaaaauua ucgaaucuca gaagcacuau     1320

uacccuuuuc uggcugagcu gaacccgaau gaaaaacggu ccagacaggc gaaguauaag     1380

cucgacgagc ugguggccuu ccgcgugccc uacuaugucg gcccuauggu uaaagcuaca     1440

gaaaacaauc agagcaacaa ugggaaguuu gccuggauga uuaaaaacga uaauaucucu     1500

gacaaacuua aaaaggagga gaucaccccc uggaacuucg auaagaaagu ggaccggaca     1560

gaaaccgcca ccaaguuuau uacaagaaug acgaccacag acaccuaccu gaucggagag     1620

ccugugcucc ccaaaaauag ccugcuguac caaaaguucg ucgugcuuaa cgaacucaau     1680

aaccugaaaa uuaauaagca gccgaucaca ccugagcaga agcaguaugu uuacgagcau     1740

gugaugaaac aaaccaagcg cgucucccug aaaaagauug cugaugcccu cgugaucaaa     1800

ggcgacuauc ccuacgcgcc ugaaaucacg gggacaaccg aucagaagaa caugaacaau     1860

ggacugucua ccuauauuga ccuugcuaag aucuuuggcg augagcugga cgauauugac     1920

cggaaagaag auuucgagaa gaucauugaa uggagcacaa ucuuugagga ccagcacauc     1980

uucgcccuca aacugaagga gauuaaaugg cugaccgaag aucagagaaa gcaaguugug     2040

aaacuucgcu accaggggug gggaaagcuc uccaagaaac ugcuggccga ccucgucgau     2100

gacaacggcc agcggaucau ugaccugcuu ugggauagca agcaggacuu caugcaaauc     2160

gugaaucaga acucuuuuaa acaggcuauu gccgagcaua auggcgcuca ccuggaagag     2220

aaggaucuca aagacaucau caacaaucug uauacaagcc cccaaaacaa gaaggccauu     2280

agacagguca uccugguggu ugaugacauu auccgcgcgg ugggguacga acccgucaau     2340

auuaugaugg aguucgcccg ggaggauagc aaagaccaua gacuuacgaa cucucggucc     2400

cgccagcucg aaaaggugua caaagauauc accaauagcu gguuugacga ugacaacguu     2460

aaggacgagc ugaaagauaa gauuaaggac aaagcuaagu ucacagauag acuguaucuc     2520

uacuuuaccc agggaggcaa agaccuguau acaggggaac cucuugauau cgacaaucug     2580

uccaacuacc aaaucgauca cauucucccg cagucuuuca uccuggacaa uagccuggau     2640

aaccgggugc uuaccuccaa gauuaacaau cagaucaaaa aggacaaccu ccccggagag     2700

uuuuucggcu cuaagaugca caauuuuugg aaacaucuga aggaacugca ccucauuagc     2760

aacaaaaagu auuacaaucu gacgcuuacc ccuaacucca ucagcaaauu caauaaccag     2820

gggagauuua ucaaucgcca acuggucgag acaagacagg ugauuaagcu cgccgcugag     2880

auccugcaua accgguauuc ugaagagaau ggcaccaaca uugugacagu uaaggccaau     2940

cugacccacc acaugcgcga aaaauucaac uuuuacaaga auagaaacgu caaugauuac     3000

caucacgcgu ucgacgccua ucuugcugcc uucgugggaa acuggcugcu ccagcaguac     3060

cccaaacuga agccuuauuu uguguacggc gauuucgcua aaacggagau cgacaaccuc     3120

aaguccuuua aucugauuua uaaauucgag caagcccauc agcuuaacga agaggacggg     3180

aagaucgcca aggauacaga cgcucugcuc ggauacauga aaaaugucua ucacuuuaag     3240

aaaaugcugg ugaccaagga acuggagaca aaccauggca aucuuuucaa acagacccug     3300

uaccccagcc acgggaagau gaagaaccuc aucaauccga aagaaaaccg gucuuccgau     3360

auuuacggag gcuauagcuc ugagaccggg gcguacguua gccugaucaa gguguuuaaa     3420

aagaauaaaa uugagaacaa gaucauuaag aucccucucc uguauauuga caaacuuaau     3480

cgccaagcca acgcuaagca guucaucaga aauuuuauca uuaaagauga cuccaacaag     3540

uucgauaucc ugagcgagaa aauuuuucuc aaucagcugg ucaucgacgg cgaugaacug     3600

uucacaauuu cuggauccgg cgaccggcag aacgcccaac agcuuauccu gagcgauaag     3660

ucccucaaga uccugucuaa aaacauuaau gugaucagca agaacgaccu cauuaucauu     3720

uucaaugaaa ucaucgauga cauuuguaaa uacaugaaga uguaugaggu ugaaaacucc     3780

gacgagacga aagccaaguu ugaggaaauc aaugauaaag accgcaugcu gaagauuauc     3840

aacgaucuuc ugcuggggcu ccacgcuaau gccucuagaa gcaaccuguc cucuauugga     3900

cuuagccccu ccuucggccg guuuuucagc gggaaugcgg gaaucaagcu gucugagaac     3960

gcuaaauuua uuuaccaguc cccuaccggc cucuucagcc gcaaggugaa aaucucugac     4020

cuguga                                                                4026


<210>  4153
<211>  4032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4153
augaccuacu aucugggccu cgacaugggg acaagcuccg ugggaugggc cgucacggau       60

gagaacuaca aucugaucca cagacaucgg aaggaccuuu ggggcauucg cgaguucgag      120

gaagcuaaaa ccgccguuga gagacggaca cagcgcaucu cuagacggcg cagacaacgg      180

gaaguggcgc gcauugggcu gcucaaggau uauuuucacg acgagaucca gaaaguggau      240

cccaacuucu accagagacu ggacaauagc aaguauuuuc cugaggauaa agaagagguc      300

gugaagggaa agaacggcau uuucgcugac gaaaauuaca ccgaucggga cuauuuugcc      360

gaguacccga caaucuucca ucuucgcaga gagcugcuca acuccaaaga accccacgau      420

guucggcugc uguaccucgc ccugcuuaau auguauaagc aucgcgggca cuuucugaac      480

accacacucg gaaccgacgg cgagaauaga aaacugucug auguguaccg ggacuucguc      540

gugaagacgg aagagguucu uggccugacc uuuccucagg aaacagacaa cgggaaaaug      600

caagagauca ugugcagcag agaguucucc cgcaagcgga aggcugaacu ccugugugcc      660

cugcucgaga uugauaaaaa gcagaaacaa cagguggcuc ugaucgaaug cguccuugga      720

cuggacaagu cugugaaaca gauguauggc accgccgugc ccgaugacuu ugauaagagc      780

gucaaaguua auuucuccga cagcucuuuu gaggaaaagg uguccgaggu ggaggaacuc      840

cuggaugagg aaaaguacga gcuuauucug uguaugaaag aacucuauga cacagcgguc      900

cugaaccaga ucauggggga uugcaauuac cugagcgagg ccagagugca ggaguaugaa      960

aagcacaaag aggaccucaa gcugcuuaaa cgcaccguua agaaguacgg aacggcugaa     1020

gaguaccagu uccuguuccg gucuuccgag aaagccagcu auaacgcuua cgugcauucu     1080

gucaauuccg gcgaaaagau uagacgcaac auggaugggc ggagcagaga cgaccucuau     1140

acagugcuga aaaagaugcu uaaaaacuac ccucaagagg augccgacug uaaguauauc     1200

cuggaagaga uugaaaagga gcaguuucuc cccaaacagc ugguuuccga uaauggagug     1260

aucccuaacc aaauccacua caaggagaug aaaaagauuc uggaaaaugc ggagcaguau     1320

cuccauuucc ugcgcgaaaa agacgagucu ggcuacaccg ucgccgaacg gauccuuaag     1380

cuguuugcuu uccagauucc guacuauauc ggccccacaa gcgagaaguc cgggaaaaac     1440

ggaggcaaug gguggguggu cagaaaggag cagggacaag ugcucccuug gaacauugaa     1500

gagaaaaucg auaugaaggc caccagcgaa accuuuaucu cucgcauggu ucggagaugc     1560

acauaccugu ccgacgagau ggugcugccc aaagcuagcc uugaauauga gucuuucugu     1620

guccucaaug agcugaacaa ucugcggauu cgcggcguga agcucccuau cacgguuaaa     1680

caggauauuu acaaggaccu guuugaaaga gggaagcggg ugaccagaaa acagaucguc     1740

aacuaucuua agggaguggg cauucugcag gagggcgaag aggaugagcu cacagggaug     1800

auggacaccu ucaauagcuc ccuggccucu uacggaaaau uuaagagcau ccugggcgaa     1860

aagauccaag aggauuccug caaacgcaug guggaacaga uuaucuucug guguacaauu     1920

uauggggacg ccaagaaauu ucuuaaggag cagcucgaag agaaauacgg agauguucug     1980

acccgggagc agaucaagcg cauucugggc uucaaacuca aggacugggg gaaccugagc     2040

aagaaauuuc uugaacugaa uggcugcgac agaaacacgg gagaggucau gucucucauc     2100

agaaugcugu gggaauccga ugacaaucug auggagcuuc ucaaggauag cucuuacaca     2160

uaucgggcug aacuggagga gcaacaggaa aaacugacca agacccucac agagauuugu     2220

cccgaagacc uggagggcua cuauuucucc gcgccgguga aacgcaugau cuggcagacc     2280

cuucugaucc ucaaggaagu ggagaagguc augggggagc cucccaaaag aguguucguu     2340

gaaaugacac gggagcacga ugaacgcaga gagaugaagg acagccgggg caaaaaguuu     2400

caggagcugu acaaaaacau uaaggaugaa gaacacaacu ggaaagccga aaucgaggac     2460

gcugaaaagg auggacgcau uagauccaag aaaauguauc uguaccuuac gcaacggggg     2520

cgcugcaugu auaccggaaa uccuaucgac cucgaggagc uguucacaac caaguacgau     2580

auugaccaua ucuaccccag acacuuugug aaagaugaca accuggacaa uaaccucguc     2640

cugguggaaa aggagaaaaa ugcccauaag ucugaucagu auccccuugg cgacgauauc     2700

uacacagggc ggcugagcug guggaaggaa cuccacagag agaaacugau uaacgacgaa     2760

aaguauaaac gccugaccgg ccgggccccu uucuccgaug agcagaaggc uggauuuauc     2820

gccagacaac uuguugagac gucucagggc accaaagggg uggcgagcau ucugaaggaa     2880

guccucccgg agacaaccac aaucguguac gcuaaggccc gcaauguuuc cgacuuccgg     2940

aacaccaaug aacugcucaa agccagaagc gugaacgauu uucaucacgc ucaggacgcc     3000

uaucugaaua uugucguggg aaacguguac uaugucaagu ucacgaauaa cccccuuaau     3060

uuuguucgcu cuggcgaggg guacaaccug gauggagugu uccggaaaaa cgugaucaga     3120

ggccaggaaa augcuuggau cgucaagccu gagaaagggg agaagaugac acucgaaacc     3180

gugaagcgcg uuauggccaa aaacacaccc cugcugaccc ggcuuuccuu ugagggcaga     3240

ggaggccugg cgaaucaaac ccucuacagc gccaaggaag cugccgggga gggauaugug     3300

ccucugaaau cuuccgacag ccggcuccag gaugucacaa aguacggcgg guucacgucu     3360

gugaccacag cuuauuuuau ucugguugag cacgacauga aaaagaagcg cgugagaacc     3420

aucgaaagcg uccccauucu uuggaaagag cggaucgaaa aggaguccgg acagcuggaa     3480

auuuacugcc gcgaggcccu cggccuggug aacccggaca ucagaguucg gaaaauccug     3540

auucagagcc uugugaagaa agauggguuc uauaugcauc ugucuggcaa gacaggaaau     3600

agauucauca ugcgcaacgc ggucgagcuc ugucugaaac aagaauggau uaauuacucc     3660

aagaagcucg agaaagaagg cugcgacgag agcaucacca aggagaaaaa cgaggagcug     3720

uaugaaauuc uuaaggagaa acacaucaau gggauuuacc ggaagagacc uaaccccaug     3780

ggagaaaagc ugcauauccg ccaggagacg uuucggaugc ugacacucga agaacagaaa     3840

aaggugcugc uuucccugcu caaucugacc aaaaucggcc cuguggaggc caaccucgaa     3900

cugauugggg agucugcuca caccggaaag augcuuauca gcaaaaauau uacagaaguc     3960

aaggaguucu accugaucca ccaguccguu accggcauuu augagaagca agugaaccug     4020

cucacagugu ga                                                         4032


<210>  4154
<211>  4032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4154
auggccacca agaaagagua caacaucggc cuggacauug ggacaacgag cgugggaugg       60

uccgucguug aaaccgauaa ucagaaggug augagaaaag gcaacaaggc ucucugggga      120

gugcggcugu ucgaggaagc cacaaccgcg gagucucgca gaaugcaacg gagcacacgc      180

agacgguaug accgcagacg ggaacgcauc aaacuucugc aggaggaguu uuccgaagag      240

auuaauaagg ucgauaagaa cuucuuucag aaacucaagg aaucuaaaua cguggagaau      300

gacaagauca acaaaaagau uguucugacc aaggaggaaa aacaggagcu uaaggauuau      360

caaaauaaau acaagacaau cuaucaccug agagacgaac ucaucaacaa ucccgagaaa      420

aaggauauuc ggcuggugua ccuggcuauc caucacauua ucaaauaucg cgggaacuuc      480

cucuaccaga augccaacuu uaauauugac aaccuggaua ucaaggacaa acuuaaugaa      540

cuguucagca uucucuccaa caacauccag gagcuggaga ucccugaaga uuacauuaau      600

aucauugacu uugaugagcu ugaagccgac cugcucaagg acaccaagaa cgaugucaaa      660

gcucugcuga ucgacaaucu cacggagcug accaacaagu cuagcgccac agaguucggc      720

aaacuuaugg uggggaauaa gggaaacauu aauaagcugc ucaugcugga gaccgauaac      780

aaaaucgaga ucuccuuuag cggcacagac uaugaugaca aguacgaaga guaucaggaa      840

gcucuuggcg agaauauuga aauccuggau auucucaaac aaaucuacga cugcauuuuc      900

cugaagaaaa ucuuuaaggg gaacaagaau accucuaucu ccagccugau gguuaaaucu      960

uucgagcagc auaagaaaga ucucaacuuu cugaaggagc uuuucaaaga caauagagaa     1020

cuguauaacg agauuuuccg gacgaagaag gaucucuguc uguacgaaaa auauaucaca     1080

aauaagauug acugcgacga guuucgcaaa gagaucaaca aacuucugga aucccucuuc     1140

agcgaugaga acauuaagca gaaucugcug gacgaauaca accucgaggu gaaggaaaga     1200

aucgagaaug gaaccuuucu gccgcggauu acaaccaccg auaacggcaa auaccccuau     1260

cagcuuaaua agucugagcu gaucaagauc auugaaaacc aagggaaaua cuauccuuuc     1320

cuccugaaua agcugaacga caauaaaaca uacaagaucg ucaaacuucu cgaguuucgc     1380

auucccuauu acgugggacc ucuggugucc gaaaagagaa gcgagaacuc cuggcuggaa     1440

cggaagauca auaacgucaa aauuacgccc uauaauuucg augacguugu ggauaaggag     1500

aaaaccgccg agaaguuuau caaaagaaug aucucucacu guacauaccu ccuggacgaa     1560

uacgcgcuug ccaacaauag cauucuguau uccgaguuca aggugaugaa cgaacucaag     1620

cagauccgcg ucaacgaucg gaaacuggag cugaaguuuc agcaacagau ucuugaagac     1680

uucuuuaaaa agaccagcgg cacaaucacc gagagaaaau ucaagaauua ccucauuucu     1740

acgggggagu auuccaugga aggaggcaac uuucgcauca caggcuacag cgcugauggg     1800

aaguucgcca auaacaugca gucuuaugug gacuucuuug gagagaaugg caucuucgcu     1860

gauaccgacu acaaccugga aaaugccgag gagauuaucg aauggauuac caucuuugag     1920

gacaaagaua uucuggaaaa gaaaguucgg gacaacuaua gcaagcucaa ugagaaccag     1980

aucaaaaagg ugcuggcgaa aaaguacucc ggguggggau cucuuagcaa gaaacugcuc     2040

aagacaaaau acuauaagga uaaagccacc gaacuguaca aguccauccu ggaccuuaug     2100

uaugagacag auaagaauuu caugcaaauu aucaacgacg auaaauacaa uuuucaggag     2160

augauuaaga aauucaacaa uaaggaagag aacaaaaagc ucaacuauag caucgucgaa     2220

gaacuggcua cgucuccugc caccaagaga ggcauuuacc aggcucugaa agugguugag     2280

gaacucgugg acuauaucgg guacgagccg aagaauauuu ccaucgaaau ggcccgcagc     2340

gaggaagaga aaguccggaa ggauucuaga aaagacuaca ucaacaaucu guauaaggga     2400

ugcaaggauu ccauugaaaa cuacaaaaag cugaaacaug agcuugacag ccacgagauc     2460

acaucccagc gccucuuucu guauuucauu caagaaggca agugucugua caccggcaca     2520

ccccuuaaua ucgaggauau ugaaaaccag ucucucuaug agaucgacca caucauuccu     2580

cggagccuga ucaaagacga uuccauugac aauaaggccc uggugcucaa ggagugcaac     2640

caggauaaaa aggcuucuua cguccugccc agcaaauuua gaaauuccaa gcagaaaacc     2700

ugguggaagc aucuuaaaga caacgggcug augagcgcga agaaguucca caaucucauc     2760

cgggaaaaau auaacgagga agauauuaau ggauuuauca accgccaacu gguggagacg     2820

agacagauca ccaagcaugu ugccaauauu cugaacaauc uuuacaaaga cacaaacgug     2880

aucuaccuga aggcugaucu cucucacaac uaucgggaaa aauacgaccu guucaaguuu     2940

agagauauua augacuauca ccaugcccac gaugccuacc ucgcugccgu ccugggcgag     3000

uauaaggagc gcuucaugaa acggaagauc aacuucgaca uggugaaaga acuuaauucc     3060

aagauuguug agauggacga agggaaaaga cgcaaccuga gauuuggaua cgugaucaau     3120

agccucgaug agaaccugaa ugacauuguc cugaagaucu cugaaaaccu uguggauaau     3180

gagaccggcg aggugcuguu cgacgcgcau gaauuuaaca agcggguuga ggauacacuc     3240

uaucgcaaug acauccugau uuccagaaaa accgaaaucc ggacggggca guucuacaag     3300

gagacaauuu acaaaaaggg caaaggaacc aucccuauua aguuuggcau gcccacagau     3360

aucuaugggg gauacagcaa caccaaugcu cgcaagcuca ugcugaucga auauaacgag     3420

ucuaaaauga agauuaucgg ccuuccgaug gcccuggccg ucagaaauaa cgacgcugag     3480

auuaacgauu acaucaaaac ccagauuaau cucaagaaaa acagcacauu caagaucaaa     3540

aaguguucca ucccuuuuga aacggagauu aucuauaaua accaaaaugu guucauuaag     3600

ggguacagca ucggacacaa aaacugcgaa auuucuaaug ccauccagcu gaagauugcu     3660

aaagacaaga ugaaacauug gaaguaugcc cugcaguacc uucugaacga uaagaaacaa     3720

uacaucgacu uugcggagaa guaugugaau gagaucuacg auuuccucau uaaccuggac     3780

cucuuucccc uguucaauaa agaacuugac aagaucaaaa acaaggucaa uuucgaggau     3840

cuggcccugg accagcagaa gaaaauuacc aaggaacucu uuaaaaugcu gcacuguaac     3900

uccgugaaug cuaaccuuag cgaguucggc cugggggauc ggaugggccg ccucuccgga     3960

uauaacauca cagacggcau uaucaucucu aagagcguua ccgggauuag agaaucccgg     4020

uacgaguuuu ga                                                         4032


<210>  4155
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4155
augagccaga acaauaacaa gaucuacaau auuggccugg acaucgggga ugccuccgug       60

ggaugggcug ucguugacga gcacuauaac cuccugaaaa gacauggcaa gcacaugugg      120

gggucucggc uuuucaccca agccaauaca gcgguggaac gcagaagcuc ccggucuacg      180

cgcagacggu acaacaaacg cagagagcgg auucgccugc ucagagaaau cauggaggau      240

auggugcugg acgucgaucc caccuuuuuc auucggcuug cuaaugugag cuuucuggac      300

caggaagaua agaaagacua ucucaaggag aacuaccauu ccaauuauaa ccuguucauc      360

gauaaggacu uuaaugauaa aacauacuau gacaaguacc cuaccaucua ccaucugcgc      420

aaacaccucu gcgagucuaa ggaaaaagag gauccgagac ugauuuaucu ugcccugcac      480

cacaucguua aguaccgggg aaacuuccuc uaugaaggcc agaaguuuag cauggacgug      540

uccaauauug aggacaaaau gaucgauguc cugcgccagu ucaacgagau uaaucuuuuu      600

gaauacgugg aggacagaaa gaaaaucgau gaaguucuga acgugcucaa ggagccccug      660

agcaaaaagc auaaagccga aaaggcuuuc gcccuguuug acacaaccaa ggauaacaaa      720

gcugccuaua aggagcucug ugcggcccug gcugggaaua aauucaacgu cacaaagaug      780

cuuaaagagg ccgaacugca cgacgaggau gaaaaggaca uuucuuuuaa guucuccgau      840

gcuaccuucg acgaugccuu uguggagaaa caaccucucc ugggagacug cguggaguuc      900

aucgaccuuc ugcaugauau cuacagcugg gucgaacucc agaauauucu gggcucugcg      960

cacacguccg agcccagcau cucugccgcu augauucagc gguaugaaga ccacaagaac     1020

gaucugaaac uccugaagga cguuaucaga aaauaccuuc cuaagaagua cuuugaggug     1080

uuccgcgaug aaaaauccaa gaaaaauaac uauuguaauu acauuaacca ucccagcaag     1140

accccugugg acgaguuuua uaaauacauc aagaagcuga ucgaaaagau ugaugacccg     1200

gaugucaaga caauccucaa uaaaauugag cuggaguccu ucaugcuuaa gcagaacucu     1260

cggaccaaug gcgccgugcc cuaucaaaug cagcuggacg aacucaacaa aauccuggag     1320

aaucagagcg uuuacuauuc cgaucugaag gacaacgaag auaaaauuag aagcauccuc     1380

acauuucgca ucccuuacua cuucgggccc cugaauauua ccaaggaccg gcaguuugac     1440

uggaucauua agaaagaggg aaaggagaac gaaagaaucc uuccuuggaa cgcuaaugag     1500

auuguggaug ucgacaaaac ggccgaugag uucaucaagc gcaugcggaa cuuuugcaca     1560

uauuuccccg acgaaccggu gauggccaaa aauucucuga ccguuuccaa guacgaagug     1620

cucaacgaga uuaauaagcu gagaaucaac gaucaccuga ucaaacgcga caugaaggau     1680

aaaaugcuuc auacacucuu uauggaccac aagagcauuu cugcuaaugc gaugaaaaag     1740

uggcugguca agaaccaaua uuucagcaau accgaugaca ucaaaauuga gggcuuccag     1800

aaggaaaacg ccuguuccac cucucugaca ccuuggaucg auuuuacgaa aauuuucggg     1860

aagaucaaug agagcaacua cgacuuuauc gagaaaauua ucuaugaugu gaccgucuuc     1920

gaagacaaga agauucuccg gagacgccug aaaaaggaau acgaccuuga ugaggagaaa     1980

aucaagaaaa uucugaagcu caaauauucc ggauggagcc ggcugucuaa gaagcugcuu     2040

uccggcauca aaacaaagua caaagacagc accagaacac ccgaaaccgu gcucgagguu     2100

auggaacgga cgaauaugaa ccugaugcaa gugaucaaug augagaagcu gggguuuaaa     2160

aagacaauug acgaugcuaa cucuaccucc gucagcggaa aguucuccua cgccgaagug     2220

caggagcucg ccggcucucc ugcuaucaaa cgcggcauuu ggcaggcccu gcuuaucguu     2280

gacgagauua agaaaaucau gaagcaugaa cccgcgcacg uguauaucga guuugcuaga     2340

aacgaagaug agaaagagcg gaaggacagc uucgucaauc agaugcugaa gcucuacaaa     2400

gauuaugacu uugaagauga gaccgaaaag gaggccaaca aacaccugaa gggggaagac     2460

gccaaaucca agauuagauc ugagcgccug aaacuuuacu auacacagau gggaaaaugc     2520

auguacaccg gcaagagccu cgacaucgau cggcuggaca cauaucaagu ggaucauauu     2580

gugccccagu cccugcucaa agacgauagc aucgacaaua agguucuggu ccuuucuucc     2640

gagaaccaga gaaaacugga ugaccucgug auuccuagcu cuauccgcaa uaagauguac     2700

ggguucuggg aaaagcuguu uaacaauaaa auuaucuccc cgaagaaauu cuacagccug     2760

aucaagacgg aguuuaacga aaaagaucag gagagauuca uuaaucggca aaucguggaa     2820

acccgccaga uuacaaagca cgucgcucag aucauugaca accauuauga gaauaccaaa     2880

gugguuacag ugagagccga ucuuucucac caguuccggg agcgcuacca caucuauaag     2940

aacagagaca ucaaugauuu ucaucacgcu caugacgccu acauugcgac cauccucgga     3000

acguauauug gccaccgguu cgaaagccug gacgccaagu acaucuaugg ggaguacaaa     3060

cgcauuuuua gaaaccaaaa gaauaaagga aaggaaauga aaaagaacaa cgauggcuuc     3120

auccucaauu ccaugcggaa caucuacgcu gacaaggaua ccggcgagau ugucugggac     3180

cccaauuaua ucgaucgcau uaaaaagugu uuuuacuaua aagacugcuu cgugacaaag     3240

aaacuggaag agaacaaugg gaccuuuuuc aacguuacag ugcugccuaa ugauaccaac     3300

agcgacaagg auaauacgcu ugccacaguc cccgugaaca aguacagauc uaauguuaac     3360

aaauauggag gcuuuuccgg ggugaauagc uucaucgucg cuauuaaggg caaaaagaaa     3420

aagggaaaga aagugaucga ggugaacaag cugaccggga uuccucucau guacaaaaau     3480

gccgacgaag agaucaagau caacuaucug aaacaggcgg aagaucugga ggagguccag     3540

auuggcaagg aaauccuuaa aaaucagcug auugagaagg acggaggccu cuacuacauc     3600

guugccccca cagaaauuau caacgcuaag caacugaucc ucaacgaguc ccagaccaaa     3660

cuggugugug aaauuuauaa ggccaugaaa uacaagaauu augauaaccu ugacucugag     3720

aaaaucauug accuguaccg gcuccugauc aauaagaugg agcuguauua cccggaauau     3780

agaaagcagc uugugaaaaa guuugaggau cgcuacgaac aacugaaagu cauuagcauc     3840

gaggaaaagu gcaacaucau uaaacagauc cucgcuaccc ugcauuguaa uuccucuauu     3900

gggaagauca uguacagcga cuucaagauu uccacaacga ucggacggcu caacggcaga     3960

accauuucuc uggaugacau cagcuucauc gccgaguccc cuacagggau guauagcaaa     4020

aaguacaaac uuuga                                                      4035


<210>  4156
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4156
augaagaaaa ccaaccccua cauccugagc cucgacauug gcacagccuc caucggguau       60

gcuugcaugg aucuggacuu caauauucuu aaguaccacg auaaagacgc caucggagug      120

cugacguuug agggcgcgga uaccgcugcc gaaagacggg gguuccgcac aucuagacgg      180

cgcaagaaca gacggauuaa acgccucgga cugcuucagg agguccuggc cccucucguu      240

aagaauccga acuauuacca acugcugaau cuccauaagu ggaaaaacag caaugaagac      300

uuuaagcaca gaucccuguc ugaggugcuu aaaauccugg gcaagaaccc caaaaccuau      360

ccuacaaucu accaucucca gcaggauauu cagaaucacc ccgaaaagaa guucgagccu      420

gagcugaucu uucuugcucu guaucaucuc gugaaauacc gggggcacuu ccuguuuaac      480

aaucugagcu ucgacaaggg aaaaaccguc gauaacgaag cccaacucuc cgagcugcuu      540

gaacaguacc agucuauuaa ucccuaugag gacagcaucu ccaacaagga uauuaaugug      600

aucguugaca cacugaaaga ugaccagcuc acccgcaacg acaagagcaa agagcugaag      660

agaaacgugu cuaagacgca cgcugaacuu uuuaaaaugc uggucggccu caaguucaaa      720

caauccauuc uguuugagaa uauggaaaac gccgaggaua ucaaggaaag cgcgaccggc      780

ucugacgcca ucucccugag cgaugacuac uaugagaauc ucucugaguu ccugacagau      840

gaacagcuua acuuuauuga guccgcuaau accuucuuca uggggaucau gcuggaccgg      900

auucucaaag gagccacaag caucuccgcu gccaaggugc gcgauuacaa gauuuuuaaa      960

aaccagcuga agaucaucaa agacauuguu uaucagaaua aguacgauaa auaucaugag     1020

uucuuuguga ccucuaagga ggacaugaga gcguaccaag aaaacccuga ugccaagacg     1080

uucggcaaac uuagcaaguu ugaccaguac cugauccacc cgaaagacca guaugcuaag     1140

cucaugaaag aucugaaggg gauugucccc aagaaauaca cagaggugcu gaaucucauc     1200

gacgagcaaa aguuccuggu gguccagaac accacagaua augccuccau uccuuaucag     1260

aacaauguuu uugaagcuga gaacauccuu cggaaucagc aaaaauacua ucccgaaauc     1320

accgaggaaa ugauuggaaa ggugaucaac cugauuagcu uccgcauccc uuacuaugug     1380

ggcccccuca ccaacaaaaa ugggcagucu gacuuugccu ggaugauuag aaaguccgau     1440

gagcagacac ugccguggaa cuucgacaaa gucaucgaua agagcaaguc ugccgagaaa     1500

uuuauucgga gaaugacgag caaguguacc uaccugcuug aagaggacgu gcucccuaaa     1560

cauucccugc uguaccagga aauggagguu cucaaugagc ugaacaaugu gcaacuucgc     1620

ggagaaaacg agcccaagca ccggauccac agacuggauc cuaaagucaa gcaguucauc     1680

uaucauaauc ucuuucagaa gaaaaagaac gugacacaca aagaccugau uacccugcuu     1740

caaaauucug aacaucgcaa cacacucaau aagaccagcg gccaaaaacu gaagguuuuc     1800

ggcacgcaga acgauuccaa guucgcuagc aaacugucua cauacaauga caugaaggcg     1860

aucuuugggu ccguggagaa caaacggcag augauugaag aacucguccu guggaucacc     1920

auuuucgagg auaaggccau ccuuaaacag aagaucggag aaaaguaucc caauauuacc     1980

gagaaacaac ugaaccagau cgugaagcuc aacuacagcg gcugggggag acugucugaa     2040

aaacugcuua caaaggaggu caauggacgc ucccucauug accugaugua uagcaccgaa     2100

aacaauuuua uggagaucau uacagacaaa acgcugggcu ucgauugcuu uaucaagaac     2160

uccaauaaag ugaagaacgc uaagaucucu uacaaagacg uugccgagcu ccggaccagc     2220

cccgcccuga agaaagggau uuggaaugcu aucaagaugg ugagagaaau uuccgaucuu     2280

uucggagccc cugagaaaau cauuaucgaa uuugcgacag aggaccaggu caagggcaag     2340

cgccaaaaau cucggagcga acugugggau gaccucguga agaaaaacaa ucugaagaga     2400

aacaaaaagu ucaagggccu guuugaggag cuuaaggcuu auccggaucu cgacuucucc     2460

aaucccaagc uguggcugua cauccaccag aacgggaaau guauguacac caagaaaccu     2520

auugaucucg aacgccugau gagcgacaca aauaaccagc uuuaugagau cgaucauauu     2580

cugccccgga ccuuuguuaa ggacgauucu aucaauaaca aggugcucgu cauucagucc     2640

gccaaccaga aaaagagcgg agacaaaaug ccucuggaaa ucuucgacaa ucacgugcug     2700

gugcccuuuu ggaagaugcu uuacgagaac gaucugauuu cuuccagcaa acucucuaag     2760

cugaugaaga aagaguucaa ugaccucgau aaggaaggcu ucauccagag acagcugguu     2820

gagacgcggc agaucauuag ccacgucucc gacuuucuua ccgaagagua uccgagcaca     2880

accguggugc cuaugaaagc caacgucgug ucugaacugc gcagaaaguu cgauaucccc     2940

aaaauucggc aacucaauga cuggcaucac gcuguugaug ccuaccugaa cggguuugug     3000

uaucaggcug ccagagucuu cuacccuaag gugaaucugu uugaguucaa cuuuaaaugg     3060

gagaagguuc gcgaaaagug gcggcagcuu ggagaguuca cagaaaauaa caaaaagaga     3120

gaguuuuucc uguuuaaacg cauggaaaau uaugugaacg agcauggcga ggggaugauc     3180

uccaagcuca uuuucgaccu ggauaauuuc aaauuuaacu acagcagaaa ggucgaaucc     3240

accgagucug cguucuacaa gcagagcgcc uuuuccccca aaaauacgac agcucaauau     3300

gacucugaua agaacaaaac cgccaucuac aaggaaauga aaaauaagcg gggcaucgug     3360

guuagcuaua cauacgugaa gaaagaccag gagcucauug gauauggcgu cuucaacgaa     3420

accguguuug aguacgugaa caaugcugac gucgauauga acacccgcgc ccugaagauc     3480

guuagaaaag cguucccuaa acuugacauu aaaaaggccc aguaucuguu uuccaucaau     3540

aagggggaug cucuccuggu gaacggacac cccuuccugu uugugucugc caaagagcgg     3600

auuaauggca agcaacuuga guucgacauc aacaugcagc gcagacugaa aaaggcucuc     3660

cggccguaca auaaaaugac agaacaggaa aaggucgggg uguacaacca guuugccgag     3720

aaaguugugc aagaguauga aaauagccug ggagccaaga cgcgcaacga gaagcucgaa     3780

cugaucagaa aucaguucaa cgaguccgau aaaagcaaug aagcucuuau ucagugcauc     3840

cuggaggucc ugaagaugac caaagugucu gcggagcggu ccguuaagcu caaaauucgc     3900

uggggcagcc agacaccuaa cacccugauc gacauuaaua acaaggccaa gaucauguac     3960

aacucuauua cagggcuuaa acaaaccaga cugaagucca ucuauaaacu cgcugaaagc     4020

aagaaugagc uguga                                                      4035


<210>  4157
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4157
augaacaaga auuacuauau cggccuggac auggggacca acagcguggg augggccguc       60

acagaucagg aguacaaucu cauuaaacug aagggcacgc acgcuugggg agccagaauc      120

uuccaagaag cgaaagacaa gaaagggacc cgggcuauuc gcuccucuag acggcgcaug      180

gauagacgga aguaucgcau ccaucuucug aaccagcucu uugcccagga gauucaggcc      240

aucgacagca cauucuuuau cagacuggcu uacuccgccu auugggaagu ugauaagaaa      300

gacaagggca uugggcggaa uauccuuuuc aaaaccaagc aagaggaauc ucaguuuuac      360

aaaaaguauc ccacaauuug gcaucugcgc aagagccucc uggagaacca gccugaugcu      420

uucuccgacc ugagauacgu guaccucgcc uuucaccaca ucaugaaaua ucggggaaau      480

uuccugcaug agggcgaaau uaagaccaac gagcuucagg ugcaacagau cgaacagcug      540

aaucaguuuc ucauuaacaa auacaaggag aucuaugaua augacgucga ggaacgcaca      600

uucaucgcga accaccaaau ugaugcccug aaagagaucc uucugaauaa gaacaccaau      660

aaaacgacca aggaaaagga gauuaaaaag cucuuugacu acgcugauug cgaaucucug      720

gagccguaua ucagcuguuu cuccaaacug gccgugggcg ggaaguuuga ccucagcaaa      780

cugggauacg agggcaacau ucaguucucu gaccccuccu ucgaagagaa ggcuagcauc      840

cuucaggaug acuuuagacu gaucgccauu gcgaaggaaa uguaugauuu cguuauccuc      900

aacgaccugc uuaaagauca accuaaucug ucuacagcca uggugcacgu cuacgagacc      960

caucggcagg accucaagaa acugaaggau auuaucauug acaucgaugc uaacaaaggg     1020

cugcagaaua acgaccgcug guacuuuaaa cucuucaagg auaaaaaugc ccccaagaac     1080

uauuccgcuu uuauccacaa aaauagcaac cagaagagac cuucuauuga cgaguucaau     1140

gacgagauca aaaagauucu gaucgagcau cggaacgaug ugucccuuga aaagcaagac     1200

gaucugaaaa ugcucuuuga gaagguugaa aauaaaacac ugcuuaagac cauugccauc     1260

cagagcacgu ccgugauucc ccaccagcug caccucgacg agcuggaaaa gaucaucgau     1320

aacgccucua caaaguaccc uuuccugagc aauauuagag acaaacucgu ccugcuuuuu     1380

accuuccgcg ugccguauua cuauggaccc cugaacgaua aguccccuua cagcaaugug     1440

gagcggaagc aguuugcuac agucaccccc uggaacuucg acgagaucuu ugaugaaaac     1500

aaaaccagag agaaguucau gaaaaagcuc acaaauaacu gcacguaucu gucuggcgaa     1560

accguuaugc cuaaaaaggc gcuggccuac gaggacuacc uuauuuauga ucgccucaau     1620

gugcugcugg ugaacgggga guaccucucc cugcaagaaa aggagaaaau cuaucauuac     1680

cuuauuagcc ggcccaagac aacccuggac cagcucaaaa aggaacuggc uaaauggcac     1740

aaucagucuc cgaucgauau cauuuucagc aacauccaug aggaaauucc uuuugaggcc     1800

uccucucacg cccaucugau gaaguccuuc gaccuugaga agcagaaaga agaacucgaa     1860

agauauauca cacuggcuac cauuuacgcc gacgauaaga aaagccugaa ggagcuccug     1920

aaaaaugaaa gccuucugga cgagaaccaa aagaauguca uccucacgcu gcccacaaag     1980

aaaugggcgc cucugucuua uaaacuucuc cagaacaucg uguacaccga ugacuacgga     2040

guugugcacu ccauucugga uaucaugaaa gagaccaauc agaacuuuca gaugguccug     2100

cacaaugaac aauaucauuu caaggagcuc auugaacagg agaaccagaa auuuacacaa     2160

gaaaagacca ucgaggaccu gauugaggaa aaacuugaga gcauccccuc ucugacacag     2220

cgcuccaucc ggcagacgcu ccugauuauc gaugacauua ucaaggcuag ccaccagaag     2280

gugcccgaaa agauuuucau cgagguuacc agaaauaacg augacaagcg caaaggccaa     2340

gauggcaacc agcuggcccg ggacaaggag cuuaauagau uucucacaaa ccugcugaaa     2400

gaugaaaagg ccaaucaguu cugugacgug ucccagcucc ugaaggaccu ugaggaaguc     2460

gauaaaguga agcugaaagg gaagcauauu uaccucuauu uuaaacaacu gggaaucgac     2520

cuguacaccg gcaagaagau cguccuuaac cagcugcuca caucuaccaa auaugauacg     2580

gaccacauua ucccucagag ccugauuaag gaugacuccc ucgauaaucu ggugcuuguu     2640

gagcgcgaag ugaaccugaa agucaagggg aaucacuacc cggugcccuc ucggauccag     2700

gacgagcaga aaaagcucug gcauuuccug cugcaaaaga aagcuauuag cgagaagaaa     2760

uauggaaacc uuaccagaac auccaagauc aaacuggaag agguugaaga uuuuguggcc     2820

cggcagauca augucauuaa cuacagcaau gugaucauuc gcgacauccu caagcugaag     2880

uacccucaca ccgauauuau cuucucuaaa ucccaauauc ccagcuuuau cagaaagcau     2940

cucaaaauug ugaagaaccg ggacguuaau gacgcucacc augccgucga ugcguaccug     3000

aacguggugu cuggcaaucu ucuguccaca accuucagca aagccuauau cagaaagaaa     3060

uacauucaga uccaggacgg ggagaaggaa acguucaaca uggagacaau ucucgagaac     3120

uauuuuaaga auaacgaaaa ucagcugaaa aucaagacca acugccugcg ccgggaugcu     3180

cuuauuacau ucaaagucga cuacuuuucu ggagaguucu auggccaaac cagacugccu     3240

aagggcaaug aaaaccucau ccccaucacc cugaaugagg auaacccgau gagcgacaca     3300

uccaaauacg ggggauacac gaaucucgcc caggcuuauc uguuugccgu gagcuaccag     3360

gaagauggcg agaugaagaa ggcgauucuu cgcguuccuu aucuguacga cacccuguau     3420

aaaaacgauu cugacaagcu ccuguccaaa gugcuugaua aucccaaggc cacaaacauc     3480

cagauucuga gaaaaaucca ccucaaucaa aagauucggu ucaacgacgg gaucuaccug     3540

cucuauacca augagaagga ucgcaacaaa uacaagaugg cuuaccagaa uuauaucgac     3600

aacgacuacc uguauuaccu ucacugggcc aacaaacauc uggaggaacu gcucgauaau     3660

caggaaaagc agacauuuau uuauaacagc aaaaaugacc aauucgaggu cuccaaggcu     3720

aagaacaaag agaucuuuca guaccugcuu cagaagacca aagccaaggu guaugauacg     3780

uguaauuaca uuguuaaagc cagagaaaca aacaucgaga uuuucgaaca gcugacccuc     3840

aaggagcaaa ucaccaugau caaucagugg auuaaaauga ugucucggga cugcgaaaag     3900

gugaaguuug augcucgcuu cacaaacuuc agcaauuguc ugcucagacu guccaacaau     3960

aucaacccua aagacucuau uaccaucauu uacgagagcc ccacaggacu uuuuaagcac     4020

gaggucaaaa ucuga                                                      4035


<210>  4158
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4158
augaagaaaa ccaaccccua cauccugagc cucgacauug gcacagccuc caucggguau       60

gcuugcaugg aucuggacuu caauauucuu aaguaccacg auaaagacgc caucggagug      120

cugacguuug agggcgcgga uaccgcugcc gaaagacggg gguuccgcac aucuagacgg      180

cgcaagaaca gacggauuaa acgccucgga cugcuucagg agguccuggc cccucucguu      240

aagaauccga acuauuacca acugcugaau cuccauaagu ggaaaaacag caaugaagac      300

uuuaagcaca gaucccuguc ugaggugcuu aaaacccugg gcaagaaccc caaaacauau      360

ccuaccaucu accaucucca gcaggauauc cagaaucacc ccgaaaagaa guucgagccu      420

gagcugauuu uucuugcucu guaucaucuc gugaaauacc gggggcacuu ccuguuuaac      480

aaucugagcu ucgacaaggg aaaaacaguc gauaacgaag cccaacucuc cgagcugcuu      540

gaacaguacc agucuaucaa ucccuaugag ggcagcauuu uuaacaagga caucaaagug      600

auuguugaua cccugaagga cgaucagcuc acgcgcaaug acaaauccaa ggagcugaag      660

agaaacguga gcaaaaccca cgcugaacuu uucaagaugc uggucggccu caaauuuaag      720

caaucuaucc uguucgagaa cauggaaaau guggaggaca uuaaagaauc cgccacaggg      780

agcgaugcga ucucucuguc cgacgauuac uaugagaacc ucagcgaguu ucugaccgac      840

gaacagcuua auuucaucga gucugccaac acauucuuua ugggaauuau gcuggaucgg      900

auccucaagg gcgcuaccuc cauuagcgcc gcuaagguuc gcgacuacaa aaucuucaag      960

aaucagcuga aacuuauuaa ggauaucgug uaucagaaca aauacgacaa guaucaugaa     1020

uuuuucguca cguccaagga ggauaugaga gccuaccaag aaaauccuga cgcgaaaaca     1080

uuugggaagc ugucuaaauu cgaucaguac cucauccacc cgaaggacca guaugccaaa     1140

cugaugaagg accugaaggg aauugugccc aaaaaguaca ccgaggugcu caaccugauc     1200

gaugagcaaa aauuucuugu cguucagaau acaaccgaca acgcuagcau uccuuaucag     1260

aauaacgugu ucgaagccga gaauauccug cggaaccagc aaaaguacua ucccgaaauu     1320

accgaggaaa ugaucggcaa agugaucaau cucauuuccu uucgcauccc uuacuauguc     1380

gggccccuga caaacaagaa cggacagagc gauuucgcuu ggaugauuag aaaaucugac     1440

gagcaggccc ugccguggaa uuuugauaag gugaucgaca aguccaaaag cgccgagaag     1500

uucauucgga gaaugacguc uaaauguacc uaccuucucg aagaggaugu ucugccuaag     1560

cauagccugc ucuaccagga aauggaggug cugaacgagc uuaauaacgu ccaacugcgc     1620

ggcgaaaaug agcccaaaca ccggauccac agacucgacc cuaaggugaa gcaguuuauu     1680

uaucauaacc uguuccagaa aaagaaaaau guuacacaca aggaucugau cacccuucuc     1740

cagaacuccg aacaucgcaa uacacugaac aaaaccucug gccaaaagcu gaagguguuc     1800

gggacgcaga augacagcaa auuugcuucc aagcucagca cauacaacga uaugaaagcg     1860

aucuucggau cugucgagaa uaagcggcag augauugagg agcuggugcu uugggucacc     1920

aucuuugagg acaaagccau ucugaagcag aagaucggcg aaaaauaucc caacauuacc     1980

gagaagcaac ucaaucagau cgugaaacug aacuacuccg gguggggaag acugagcgaa     2040

aaacuucuca caaaagaggu uaacggccgc ucucugaucg aucugaugua uuccaccgaa     2100

aauaacuuca uggagauuau cacagacaag acgcucgggu uugacugcuu cauuaaaaau     2160

agcaacaagg ugaagaaugc uaaaaucucc uacaaggaug ucgccgagcu gcggaccucu     2220

cccgcccuua aaaagggaau uuggaacgcu aucaaaaugg ugagagaaau cagcaaucug     2280

uuuggcgccc cugagaagau uaucauugaa uuugcgacag aggaccaggu uaagggcaaa     2340

cgccaaaagu cccggucuga acucugggau gaccugguga aaaagaacaa ucugaaaaga     2400

aacaaggagu uuaaggggcu uuucgaggaa aucaaggcuu auccggaucu cgacuuuagc     2460

aaucccaagc uguggcugua cauucaccag aacggaaaau guauguacac caagaaaccu     2520

aucgaucucg agcgccugcu uuccgacaca aauaaccagc uguaugaaau cgaucauauu     2580

cucccccgga ccuucgucaa ggacgauagc aucaauaaca aggugcuggu gauucagucu     2640

gccaaccaga aaaaguccgg cgacaaaaug ccucuggaga ucuuugauaa ucacguucuu     2700

guccccuucu ggaagaugcu guacgagaac gaccucauuu cuagcuccaa acugagcaag     2760

cucaugaaga aagaguucaa ugaccuggau aaggaagggu uuauccaaag acagcuugug     2820

gaaacgcggc agauuaucuc ucacgugagc gacuuccuga ccgaggaaua uccguccaca     2880

accgucgugc cuaugaaagc caacguugug agcgagcucc gcagaaaguu ugauaucccc     2940

aaaauucggc aacugaauga cuggcaucac gcugucgaug ccuaccugaa cggauucgug     3000

uaucaggcug ccagaguuuu cuacccuaag gugaaucuuu ucgaguuuaa cuucaaaugg     3060

gaaaaggucc gcgagaagug gcggcagcug ggcgaauuua cagagaauaa caaaaagaga     3120

gaguucuuuc ucuucaaacg cauggaaaau uaugugaacg agcaugggga aggcaugauc     3180

ucuaagcuga uuuucgaccu cgauaauuuu aaauucaacu acuccagaaa gguugagagc     3240

accgaauccg cguuuuacaa gcagucugcc uucagcccca aaaauacgac agcucaauau     3300

gacuccgaua agaacaaaac cgccaucuac aaggagauga aaaauaagcg gggaauugug     3360

gucucuuaua cauacgugaa gaaagaccag gaacugaucg gcuauggggu guuuaacgag     3420

accgucuucg aguacguuaa caaugcugau guggacauga acacccgcgc ccuuaagauc     3480

gugagaaaag cguuuccuaa gcuggacauu aaaaaggucc aguaucucuu cagcaucaau     3540

aagggagaua cacugcuggu gaacggccac cccuuucuuu ucguuuccgc cuacgaacgg     3600

auuaauggga aacaacugga guuugacauc gcuaugcagc gcaagauuaa acaggcccuc     3660

ucuccguaca acaauaugac ggaacaggag aagguggagu ucuauaacca auuugcugaa     3720

aaagucaucg aggaguucag aaauagcauc ggacugcagu cccgggaucg caaggugaac     3780

cucauuaaaa aucuguucaa caauaccgaa aagagcaacg acucuaugac acaguccauc     3840

cuugaaguuc ugaagaugac caaagugagc gccgaaagau cuaaggagcu gaaaauucgg     3900

uggaauggcc agacaccuaa caccuuuauc gauacgaaga acaaugccga aauucaauac     3960

aaauccauca cagggcucaa gaccaccaag cccaaaagca uuuucaagcu ggcugagucu     4020

aaaaacgaac uuuga                                                      4035


<210>  4159
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4159
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

guuacagaug acuauaaggu gccugcuaaa aagaugaaag ugcucggcaa uacggauaag      120

ucucacauca agaaaaaccu gcuuggggcc cuccuguucg acagcggaaa uaccgcggag      180

gauagacggc ugaagcgcac agcuagacgg cgcuacacca gacggcgcaa ccggauucuu      240

uaucugcagg aaaucuuuuc cgaggaaaug ggcaaagucg acgauucuuu cuuucauaga      300

cucgaggaca gcuuccuggu gacagaagau aagcgcgggg agagacaccc gauuuuugga      360

aaucuggagg aagagguuaa auaccaugaa aacuucccca ccaucuauca ccuccggcaa      420

uaccuggccg acaauccuga gaagacagau cuucgccugg uguaucucgc ccuggcucau      480

aucauuaagu uuggcggcca cuuccuuauc gaggggaaau uugacaccag aaacaaugau      540

guccagcggc uguuccagga auuucucgcc guguacgaca acacguucga aaauuccucu      600

cugcaggaac aaaacguuca gguggaggaa auucugaccg acaagaucag caaauccgcu      660

aagaaagauc gcguccucaa gcuguuuccc aaugagaaaa gcaacggaag auucgccgag      720

uuccuuaagc ugauuguggg caaucaggcg gacuuuaaga aacacuucga acucgaggaa      780

aaggccccuc ugcaguuuuc uaaagauauc uacgaggagg agcuugaggu gcugcucgcu      840

caaauugggg acaacuaugc cgaacuguuc cuguccgcua agaaacucua cgauagcauc      900

cugcuuucug gaauccugac agucaccgac guuuccacaa aggcuccccu cagcgcgucu      960

augauucagc gguauaacga gcaucagaug gaucuggccc agcuuaagca auuuaucaga     1020

cagaaacugu ccgacaagua caaugaagug uucagcgaug uguccaaaga cggcuaugcu     1080

ggguacauug auggaaagac caaccaggag gccuuuuaua aauaccucaa gggccugcug     1140

aauaagaucg aaggcucugg guacuuccuc gacaaaauug agcgcgagga cuuucugcgg     1200

aagcaaagaa cguucgauaa cggaagcauc ccucaccaga uccaucuuca ggaaaugcgc     1260

gcuauuaucc ggagacaggc cgaguuuuau ccguuccugg ccgacaauca agaucgcauu     1320

gaaaagaucc ucacauuucg gauucccuac uaugucggcc cucuggcuag agggaagucc     1380

gacuucgcgu ggcugagccg caaaucugcc gauaagauca cccccuggaa cuucgacgag     1440

aucguggaua aggaguccag cguugaagcc uuuauuaauc ggaugacaaa cuacgaccuu     1500

uaucucccua aucagaaagu gcugcccaag cacucucugc ucuacgagaa auucaccguc     1560

uauaacgaac ugaccaaggu gaaauacaag acagagcagg gaaaaacggc cuuuuucgau     1620

gccaauauga agcaggaaau cuuugacggc guuuucaagg uguacagaaa agucaccaag     1680

gauaaacuua uggacuuucu ggagaaggag uucgaugaau uucgcauugu ggaccucaca     1740

gggcuggaca aagagaacaa ggcuuucaau gccagcuaug gaaccuacca cgaucugcgg     1800

aagauccuug acaaagauuu ucucgacaac uccaagaaug agaaaauucu ggaagauauc     1860

guccugacac ucacccuguu cgaagacaga gagaugauuc ggaagcgccu uaaaaacuau     1920

ucugaucugc ucacgaagga gcaacugaag aaacuggaaa gacggcauua cacaggcugg     1980

ggcagacuua gcgcggagcu cauccacggg auccgcaaca aggaaucccg gaaaaccauu     2040

cuggacuauc ugaucgauga cggaaauagc aacagaaauu uuaugcagcu cauuaacgau     2100

gacgcucugu cuuucaagga ggaaaucgcc aaagcccagg ugauuggcga gaccgauaau     2160

cuuaaccagg uuguguccga caucgcuggg agcccggcca ucaagaaggg cauucugcaa     2220

ucucucaaaa ucgucgacga gcuggugaag auuaugggac aucagccuga aaauaucguu     2280

guggagaugg cucgcgaaaa ccaguucaca aaucagggga gacggaacuc ccaacagcgc     2340

cugaaaggcc uuaccgauag cauuaaggag uuuggauccc agauccucaa agagcacccc     2400

gucgaaaauu cucaacugca gaacgacaga cuguuccucu acuaucugca gaauggccgg     2460

gauauguaca caggggagga acuugacauc gauuaccuga gccaguauga cauugaucau     2520

aucauuccuc aagccuuuau caaggacaac uccauugaua aucgcgugcu cacgucuagc     2580

aaagagaaca gaggaaaguc cgacgaugug cccagcaaga acguuguccg gaaaaugaag     2640

ucuuacuggu ccaaacugcu gagcgcgaaa cuuaucaccc agcgcaaauu cgacaaucug     2700

acaaaggccg aaagaggcgg gcucaccgac gaugacaagg cuggauuuau caaacggcag     2760

cugguggaga cacgccagau uaccaagcac guggccagaa uccucgauga gcgguuccac     2820

acggaaaccg acgagaacaa uaaaaagauu agacaaguca aaaucgugac acugaagucu     2880

aaccuuguuu ccaauuuucg caaggaguuc gaacuguaua aagugcggga aauuaacgau     2940

uaccaucacg cucaugacgc cuaucucaau gcggucaucg gcaaggcccu gcugggggug     3000

uacccccagc uugagccuga guuuguuuau ggcgauuacc cgcacuucca cggacauaaa     3060

gaaaacaagg cuaccgccaa aaaguuuuuc uacagcaaua uuaugaacuu cuuuaagaaa     3120

gacgauguga gaacagacaa gaauggcgag aucaucugga aaaaggauga acacauuucu     3180

aacaucaaaa agguccugag cuauccccag gugaauauug uuaagaaagu ggaggaacag     3240

accgggggau ucuccaagga gagcauccuc ccuaaaggca acucugacaa gcugauuccc     3300

cgcaaaacga agaaauuuua cugggauaca aagaaguaug ggggauucga cuccccuauc     3360

gucgcuuaca gcauccucgu gauugccgac aucgagaaag gcaaguccaa aaagcugaaa     3420

accgugaagg cccuugucgg gguuacaauu auggaaaaga ugaccuuuga gcgggauccc     3480

guggcuuucc uggaaagaaa aggcuaucgc aaugugcaag aggagaacau cauuaagcuc     3540

ccgaaauacu cucuguuuaa gcuggaaaac ggacggaaaa gacuucuggc gagcgcccgc     3600

gagcuccaga agggcaauga aaucguccug ccuaaccauc ucgggacccu gcuuuaucac     3660

gcuaagaaua uccauaaagu ggacgagccc aagcaccugg auuacguuga caaacacaag     3720

gaugaguuca aagaacugcu cgacgugguc uccaacuuuu cuaagaagua cacacuggcc     3780

gagggaaauc uugagaaaau uaaggaacug uaugcccaga acaauggcga agaucucaaa     3840

gagcuggcua gcuccuucau caaccuccug acguuuaccg ccauuggggc gccugcuaca     3900

uucaaguuuu ucgacaaaaa uaucgaucgg aagagauaca ccucuacaac cgaaauucuu     3960

aacgccacgc ugauccauca aagcauuaca ggacuguaug agacccggau cgaccucucc     4020

aaacugggcg gggauuga                                                   4038


<210>  4160
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4160
auggagaacu acaauaucgg ccuggacauu gggaccacaa gcgugggaug ggccgucguu       60

aacgaugcua cgaauaaggu gaugagaaaa ggcgggaagg cccucugggg agugcggcug      120

uucgaccccg cggucaccgc ugaagaucgc agagccaaac ggggcacacg cagacgguau      180

gaccgcagac gggagcgcau caaacuucug caggaugaau uuaaagacga gauuaacaag      240

guggauucca auuucuuucu caagcuggaa gagucuaaau acaccagcaa ggacaucaaa      300

aacaagacag uucuuauuuc cauggaggaa gagaaagcca ucaagaagua uaaucaagaa      360

uacccuacca ucuaucaccu gagacagaaa cucaugaacu cuaaugagca gauggauauu      420

cggcuggugu accuggcuau ccaucacauu aucaaguauc gcgggaacuu cauuuacgag      480

ggaaauagcu uuuccgucaa caaucucaac aucgacaaua aacugaagga aauuuuccuu      540

ucucugagcg aggaugugga cgaacucgau aucuccgagg aaugcaucaa caacauuaau      600

uacaaacagc uggccaacau cuuuaaugag caaagcaaga acgaccuuaa aguuaagcug      660

acaucugagc ucaccuccua uagccugaau aagaacuuca uuucugaauu uuguaaacug      720

aucauuggca auaaguucuc cgcuaaaaag cucuuuaacc uggagacgga caaugaugug      780

agccuuucuu ucgacggcac cgauuuugaa gacaaagccg gggaucugga gucccuccug      840

aacgacuacc uugaaguccu ggagcagcuc aaggagcugu augauauggu guuccugaag      900

aaaaucuucg gaggcagcaa ggaaacaacc aucuccgggc ucauggugga gagauacaau      960

cagcauaaaa aggaccugaa acuucugaag gauacauuuu cuagcgaccg gaagauuuau     1020

aacaaaaucu uccgcuccaa gaaagauauu aaggacgaca aagauaugug ccucuacgac     1080

cuguauaucc acaauaaacu ugaaaccacg gauuuucuga aggagauuaa aaaguacguc     1140

aacacagaca auuucaucau caccgaugaa gacuuucuca aaagacugga gaacaacgag     1200

guucugccgc ggauuacaag caaggaaaau ggaaaauacc ccuaucagcu caacaaggau     1260

gagcuggugu cuaucauuga acaucaaggc aaacacuacc cuuuccuucu gaauaagacc     1320

gacgauggga ucuauaagcu cgugagacug cuggaguuuc gcauucccua cuucgucgga     1380

ccucuugugu ccaaagacau gagcgagaac gcguggaugc uccggaauga aggcaugcag     1440

ggcguucaca ucacccccua uaacuuuaag aaagugauua aucuggauaa gacagccgag     1500

aucuucauca aaagaaugau uucucauugu acguaccugc ucgacgaaga ggcucugccu     1560

aacaauagca uccuuuauuc cgaauuuaag guccugaacg agcucaagca gauuaaagug     1620

aauggggagc accugaccaa ggaugaacag cauaaaauca uugaggaacu guucaugaag     1680

acaucuggaa ccaucacaga gaacaaauuu cgcgaguucc uucucaauac ccuggacuac     1740

aacuucuacg aaaaugagcu gaagaucacg ggcuauagcg ccgacugcaa guuugcuaac     1800

aacaugcaau ccuacauuga uuucuuuggg gaaaauggaa ucuucgaggg cacaagcuau     1860

aacaccaaug acgccgaaaa gauuaucgag uggauuacca ucuuugauga caaggauauc     1920

cucaaacgga agguugaaaa cgaguacccg gagcugaaau cuaaucaggu gaagaacauu     1980

cuuaauaaga aauauuccgg guggggaaga cugagccgca agcuccugcu ggaccuucgg     2040

guccucgaua aagaaacaga cauccccaag ucuauuaugg aucugaugua cgagaccgac     2100

gaaaacuuca ugcagaucau uaauaacgau aaauauaauu uucaaaagcu gaucgcgaaa     2160

gagaacaaga ucgagaauac auccaacgac cucagcuacg auguggucga aaaucuggcc     2220

acguccccug cuaccaagag aggcauuuac caggcccuua aagugguuaa ggagaucaca     2280

accuauaugg gcuacgaacc caaaaacauu augguggaga uggcucgcgg ggacgaaaag     2340

aaaauccgga aggacgauag aaagaaauau cugcagaagc ucuacgacaa cauuaaagau     2400

gaggucgacu cuaauuauaa gaaccugaaa aaggagcugg ccaaggaaga gaaaaucgau     2460

acagacaaac uuuaccucua uuaccugcag gaaggaaagu gucuguacug cggcaagccu     2520

auuaaugugg augaccucaa caccaaaaau agccuguaug agaucgauca cauccuuccc     2580

cgcacgcuga uuaaggacaa cuccugggau aaucggguuc ucguguguag agggugcaac     2640

caaaagaaag ccgcuucucu gguccugccg aaggaguucc ggaaugacua caccaaaagc     2700

aucuggauuc gccuuaagaa caauaaacug auguccaaca agaaguuuua uaaucucaca     2760

agaaaaagcu acucugaaga ggacaucaag ggauucauua accggcagcu gguggaaacc     2820

agacagauca caaaacacgu ggcgaauauc cucaacuguu uuuauccuaa gaccaaaauu     2880

guuuaccuga aggccaauau cucccauagc uaucgcgaua aauacgaccu uuucaaguuu     2940

cgggauauua acgacuacca ccaugcucac gaugccuauc uggccgcuac gcucggcucu     3000

uaccaggaga aguaucugca uuacgaccug gauuauaaca aaaucaauga guucaacgaa     3060

aaacuuauua agaaugaaga cuacaagaga cugcacuaug ggaucgucgu gaacucccuc     3120

gaugaggagu uccaaagcgc cuuugcggaa aucgcucccc gccugauuaa ugaggaaaca     3180

ggcgagcucc uguucaacgc caaaaaguuu aaugaccuug uggaggauac ccuguacaga     3240

aacgacaucc ucauuucucg gaagacagaa auccgcaccg gaaaacugua caaggagacc     3300

auuuauaaaa gaggcaaggg gaauaucaaa auuaaggaaa acaugccuac agagacguac     3360

ggaggcuaua gcaauaugga aaccuccuac cugacacuug ucaaguaugg gaacaaaacc     3420

aagcugaucg gaauccccau gagcauugcc aauaacaaag augugaaggu uaaacucgac     3480

uucaucaagg agcaccugga ccucaagucu cuggauaaua uugugauccu uaaagacaac     3540

auucccuacg augucuccau caacuauaag aaucaggacg uguacguuaa aggcuacagc     3600

acagcuaaca agaauugcga gcuguccaac gcccaucagc ugaaaaucaa gaaagauaug     3660

augaagaagu guaaauaugu gcucaauaag gucuacaaaa acaaguauga cgauauuacc     3720

aauauggaca ucgaaaacgc uauuacguuu aucaauuacc uguucgagau uaaaaucgaa     3780

uuuccucuuu ucucuaagga gaucacaaag auucaggaua acaucaaucu gaccgaaugc     3840

agcuuugagc aacucgccaa cauuaucaaa cagcugcuca uuaucuauca cugcaauucc     3900

aagaacggga aucugaaaga guucggccuu ucugaccgga uuggacgccu gagcggcgcg     3960

aacgugaccg ggggagauuu uaucuucaca uccguuaccg gccugaagga aaagacagcc     4020

ucuuacgacg gggaguga                                                   4038


<210>  4161
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4161
augguggaga aguacggcau cgggcuggac auuggaacca gcacacucgg cuggacgucc       60

gucgaugaaa acgggaaaau ccagagacug aagggacaaa ccgcccuugg cacacggcug      120

uucaaugagg ggcagaccgc ugaagaacgc agaggauuuc ggacaacccg cagacggauu      180

cagcgcagac gguggcgccu cagacugcuu cgggaaaucu ucgacgagcc cauuggcucu      240

guugaugccg gcuuuuucgc gcgccagaaa augagcuccc ugucuccuca agacgauaga      300

uuugaggaca cauaucggcu uuucaacaau cagaccgaua aggcuuuuca cgacgaauac      360

ccgacgaccu aucaucugcg ccaggcccug augaacagcg auagacaguu cgacauccgg      420

gagaucuacc ucgccauuca ccauaucgug aaauauagag ggaauuuucu gcgcuccgga      480

aagcccgcug auuucaagac aggcaaacuu gaccugaagg aaaaauuuga ggagauuaac      540

gccgauuggc aaacccucau cucugaagac gcuccucagc ugcccgugga cgaucuuaca      600

accauuaagg ccguccuggu ggacgggagc cgguccagaa gcgaucgcca gcggggacuc      660

guuaaacugu ggauggcgaa ugagacggug gaaaagaacu ggaagccugu caugacagag      720

cuggccaaag cuauccucgg ccugaagacc aaacuuuacc ugcucacagg gcuggaccuu      780

cccaaggaag aucagaaagc cuggaccuuc ucucuggaca cccucgagga ucaccaagga      840

gacauugauu ccuuuuucga cgaucaugcu cagcagcuga ucgacagacu ggccgagcuc      900

cgcgcgauga uccaacugac agaaauuauc ccuagcggcc uuaauuucuc ucaguccaug      960

gugcagcggu augaggccca cagaagccac cuggaaaugu acaagcagua cgcucucgac     1020

caaacggauc cgaaacgcac caaggcccug cgggcugccu auaacgccua cauugaccag     1080

caugagaucg cugaugacuu uuauacagcg guuaagagag ugaccaaaga ugaccagucu     1140

gaaacagccg aggagauuaa ucgccuuauc ggccuggaac aguucaugcc caagcaacgg     1200

accaaagaga acggggucau cccuuaccag gugcaccagc aggaacucga ucaaauuauc     1260

gcucaucaga agaaauauua ccccuuucug ggaacggaga auccugugau ugaccacaga     1320

gaaucccagc ccuauaugcu ggaugagcuc gucagcuucc ggguuccgua cuacgugggc     1380

ccuauggugg ccgccaaccc cgagcugaag acaaccgaaa auccuuuugc uuggaugauc     1440

cgcaaggccc agggggagau uacccccugg aacuucgacg aaaaagucga uagaaaggga     1500

uccgcgacag cuuuuaucga gcggaugaaa accacagaca cguaucuucu gggcgaggau     1560

gugcuccccc aacagucucu gcuguaccag agauucaugg uucuuaacga acucaauaac     1620

auccgcauug acgacgagcu gcugaccaca aaucaaaagc aguggguguu ugaucggguc     1680

uucaaaggga agggaaccau cagcgccaag caccuccagg acgcccugaa acaucacggc     1740

gaaacagaug gccuuccuaa gauuuccggg cuggcugacc cgaccaaauu uaacagcgcc     1800

cucacgucuu auagagauuu ccagaagugg cugcccaaug acaucgaucg caaagacaga     1860

accgcugaua uugagacaau cauuaacugg uccaccgugu uugaagacac acauaucuuc     1920

guugagaagc ugaaggagau cgaauggcuu accgccgauc aacggcgcag aauugcgagc     1980

aaucgguacc gcggaugggg cagacucucu aaacaucugc ugacggagau caaggacgaa     2040

caggggcuca gcauuaugga ucagcugugg cggacacagc acaacuucau gcaaaucgug     2100

cacgagccug acuaugccaa acagauugaa gcugccaaua ccgucggaau ggcuaagcag     2160

gacaucggca cagugaucaa cgaucuuuac accucucccc agaauaaaaa ggccauucgc     2220

caaguccugg cggugguuaa agacauccag aacgcccacc augggguggc uccuuccugg     2280

auuuuuaugg aggccgcuag aggcgccgau cagaaucccc aacggagccg ccagagacag     2340

cggcagcucc aagaccugua ugccaagacc gcugcggauc uggucgacac agaggugcag     2400

caggaacuua cggauaagau caaagacaag gccaacuuca ccgauagacu caugcuguac     2460

uuucugcaga auggacaaga cauguacucc aaagagaagc ucaacauuga ucgccugagc     2520

gaauaugaca ucgaccacau ccuuccgcag gcucugauua aagaugacuc ucucgauaac     2580

cggguucugg ugcaucaguc caucaauaga aacaagagcg acacauucgc cucugagaag     2640

uacggcgcca aaaugcaggg ggcuuggcac caauggcgcg ccgcgggacu gauuuccgcu     2700

cggaagcuca gaaaucucga aaugcgcccu gaugagauca gcaaauaugc caccggcuuu     2760

auucggagac agcuggucga gacacgccag gugaucaagc uggugaccga aauccucucc     2820

caccaguaca accccgagga aacgaaacug guuucuguca aggccgggcu uagccaugag     2880

cugcggagag aggcuggacu cccuaagcug cgggaccuga augauuauca ccaugccauu     2940

gacgcguacc uugcugcccg caucggcaca uaucugcuca aaagauaccc ccggcuggaa     3000

cgcuucuuug uguacgggca auuccagacc uccagacucg aucugcggag auuuaacuuc     3060

auucacgacc uugugaaguc uaaacagggc gucaucgugg auagcgacac cggagagauu     3120

cugugggauc gccaaaagga caucgacuau auuaaacauc ucaugggccu gaagcaucug     3180

cuuguuacac acgaagugua cgauuccgcc gcugagaugu augcccagac caucuuuaag     3240

gcuagcgaca augauucuaa aguccugauc ccuacaaaga acgaccggcc cacggccauu     3300

uacgggggau auaccaaaag acgcaauggc uaccucgcga uccugaagau uacagagaaa     3360

aaggccgaga ccuaucgggu gguuggggug ccgacacucu acauguccca gcuggcuacc     3420

gccagaagcc acucugucga ggcugaaaaa gcccuucuga agucccucau ggccccugag     3480

auccugagcg aaucuaaggu gguuagcuuc gagguggucg ugucccacgu gcaucugaac     3540

cagcuugucc gcgauacggu uuuuggaccc gcucacuacu ucggccuggg gcccuuccgg     3600

uaugugagaa auuuucauca acucgugcug ccuuucaaag accaggaaau ucucgucaag     3660

cgcaccccga cagaugagga gcugaacacc guguuugaag acaucugcug gcagguugag     3720

aauuacuucg cgguguuuga uaucaacggc uucagaaaug cccuuaaaaa gagcauugac     3780

aaauuugaac agcugcccau cgagaacguc uucaaggaua agaaacuggu gacacaagga     3840

aagaaagaag uucucgcuuu ugugaugcag ggccugcacg ccaacgccuc uuaucggaau     3900

cuuaaggucu cccuggggau uaaaaccccu cucggacagc ugcagcucac aaaguucaug     3960

cugagccgcg acgcugagcu uaucgaugag ucccccaccg gccuguuuag acggauucgc     4020

agacugaagg accucuga                                                   4038


<210>  4162
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4162
augggcaaca agcugagaaa uguggacgau uacuaucucg ggcuggacau cggaaccgag       60

agcgucggcu gggccguuac agauaaauac uaugugauuc ccaaguucaa agggaacgcu      120

auguggggag ugcaccuuuu ugacgaaggc gagacguccg ucaagcggcg cggguucaga      180

ucugcccggc gcagaaccca gcggaaacgc gaaagacugg cgcuccugga ggcucuuuuu      240

gaugaagaga ucugcaagaa ggacuguaca uucuuucggc gccugcauga gagcgaucuc      300

uggccugaag acaaauccgg agaugacaag uacgccgugu ucgccgauga gaauuauacc      360

gacaaagauu uucacaagga auacccgaca auuuaucauc ugagauacga gcugaucaaa      420

aacaaggagc cccacgacgu uaagcucgug uuccuggcuc uccaucacau uaucaaaaau      480

cggggccacu uucuguucga cucucucagc uccgccgaag auuuuaacuc ugcuuacgag      540

accaugugca gcuaucuguc cgacaaugaa auccaacuua acuguagcga uauugagcag      600

uucaaggcca uccugaaaga ccgcagacag ggcaagauug aaaagacaaa gaaucucgcg      660

ucucuguuug ggaucaccaa aaaguccgau ccugucagcu acgccgugau ggcuguucug      720

ucuggaucca cggugaagau gugcgaccuc uucggcgaug acgagcugaa aaccgucgag      780

aagaacaaua uuagcuucgc cucuggguau gaagagaacg aagagaucua cgcuuccgug      840

cuuggagauc gguuugaacu ggugaaaauu cucaaggccg ucuaugacug ggcgguucug      900

gccgauaucc uugacggcaa aaaguacauc agcgaggcua agguggccgu guaugagaaa      960

cauaagaaug accugaaaac acucaagaaa uacgucaaga ccuaccugcc cgaaaaguau     1020

aacgagauuu ucaaaaucuc cgaugggaag aaagacaauu acguggcuua uucuggacac     1080

auuaaggaaa gcggcaaaac aggcgagcug aagaagaccu guaacuccga aaacuuuugc     1140

gccuaccuca aaaagacgcu ggggaaaaau gccgaucuua gcuauaagga gauguucgac     1200

gagaucgaaa acaaaacauu uaugccuaag cagguuacca aaucuaaugg aguggucccc     1260

augcaaguga acgaggagga acugaaggcu auucucgaua augcgaagga cuaccugucc     1320

uuccuuaaca gcugugauga gaauggcguu ucuguguaug acaaaaucau caagauuuuu     1380

gaauaccgca ucccuuacua ugucgggccg cugaacacac auaccgagaa uagcuggcuc     1440

aaaagaaagg auggagugaa aauuuacccc uggaacuucg acgaugucgu ggacaucgau     1500

uccucugccg aaaaguuuau uaauaaccug accagcaagu gcacauaucu gccugagugu     1560

gacguuaucc ccaaaugcuc ccuccuguac agcaaguuca cggugcuuaa ugaaaucaac     1620

aaucugaaac ucaacggcga ggacauuccu gucgagcuga agcaggggau cuauaaagau     1680

cuguuuauga cccggagaaa ggugacaguu aagcagauua aagacuaccu uaaggcucgc     1740

ggauguuaug auaacaauga cgugcucucu ggcaucgaua ccacagucaa auccagccug     1800

aagucuuacu ucgacuuuaa aggcuucgaa ucccugaccu ucgaugacaa ggagagcauu     1860

aucgccuccu cugugauuuu uggggaugac aagaaacucc ugaagaaacg gcuuaagaaa     1920

cuguuccccg aaaagcucac ggaugaggac aucaaaaaga ucugcaagcu gaaauacaca     1980

ggauggagca gacuguccaa ggaauuucuu ugcgaugugg aguucaccua uaaaaagacc     2040

ggcgagguua ugaacgucau uacagcccuc ugggaaacca auuacaaccu gaugcagauc     2100

cuguauucug aggacuuuug uugcgacggg gaugagaaaa agaaucucaa cgacaagcug     2160

gaugaacuua gcuacacagg agaccaacac ucccugcgca aagcugugga ugcccucuau     2220

gugagcccga aggucaaacg gccuauucug agagcgcugc uuaucgcuaa ggaaauugag     2280

aaaauccagg agagaccccc uaagaagauc uucguggaag uugcccgggg cauugacaau     2340

agagaggaac gccaccggga uucucgcaaa aaccgccuca uggagcugua caagaauugu     2400

aaaaaggagg ccgaaaaauu uugccccuau uucgacgcuc uguccaagaa gcucagcgau     2460

gaggacgaau cuaaacugcg gagagauaaa cuuuacuuuu acuauaucca gcuggggaaa     2520

uguauguaca cgggcgagga aauuucccuc ggagagcuga ugaacggcaa ugggcgguuc     2580

gacaucgacc auauuuaucc ccgcagcaag aucaaagaug acucucugac caacaaggug     2640

cuugucaaua agacagugaa caauaacaaa accaacguuu acccuaucga ugccgcuauu     2700

cagagcaaga uguccccgua uuggcaaaug cucagaagcu cuggacugau cuccgaggaa     2760

acauacaaac ggcugaccag aaagacgccc cucaccgacg augagcugag cgccuuugug     2820

ucccgccagc uuacagaaac ccagcaaucu acaaaagcgg ucgcccacau ucuggagcag     2880

cucuauccua agcccaaaac cgaaaucgug uacguuaagg cugcccuggu gcaugcuuuc     2940

cggcagagca augacaugcu gaagugcaga gaggucaacg aucuucacca ugccaaagac     3000

gcguaccuga auauuguggu gggcaacgcc uauaauguca agguuacgca caacaaagug     3060

aauuuuauca aggggcucca gaacaaugau aaaggauaca cagugaaccc ugagaaguuc     3120

uucucccacg acauccccgg cgcuuggucu gucaaggaua gcaugaauac cgugcgcaga     3180

uuuaugaaca aaaauaacau ucgguauaca cgcuucccga gaaagaaaca agggggccug     3240

uuuaagauca auccucucaa aaagggaaac ggccagguuc cccugaaggc cuccggggcu     3300

cggucuugua uugaaaaaua cggaggcuau gacaaggcca ccagcgccua cuucuccuau     3360

guggaguaca ccgauaaaaa ggggaaagcu guccgccaga uguuuccugu gaaccuuuac     3420

gaaguuaagg aguaugaagc ggacaugcag ggauucguga caagcagaau cgccgaggcu     3480

ucuaauguca agauucugau ccccugcgug aaauacgacg ccuguauuuc cuuugauggc     3540

ugccggaugu ucaucacgaa gaaaaccggg aaccgcaucg gcuauaagcc uggaaugcaa     3600

cucguucugg gcuacaaaua ugagaaguac auuagaaaua ucgaaaacua ucugacaaaa     3660

cauaaggacc gggagauuau cccccgcgau aauauuagcg ccgaagagaa caucgagcuu     3720

uacgacaucc ugaccgggaa gaugcucaau acagugcugu cugucaaauu uuccggaauu     3780

ggcaagaaac ucgaaaagag acgggagaaa uucgugcugu guagcccgga ggaacagugc     3840

ugcgugcuua acgaaauccu gaagauucuc cacgcuaaug uccugucugg ggaucugucc     3900

cuuaucggag agagcccuaa caccggcacg augaagacaa augccaaauu uuccgagauu     3960

aaggacguuc auagcaucaa acugaucaac cagucuguga ccgggcuuuu cgaacaggag     4020

auugaucugc ucaaguga                                                   4038


<210>  4163
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4163
augggcaagc ccuacagcau cgggcuggac auuggaacca acuccguggg cugggccguc       60

guuacagaug acuauaaagu gccugcuaag aaaaugaagg ugcucgggaa uacggauaaa      120

cagucuauca agaagaaccu gcuuggagcc cuccuguucg acagcggcga gaccgcggaa      180

gcuacaagac ugaaacggac cgcccgcaga cgguacacac gcagaaagaa ucggcuucgc      240

uaucugcaag agauuuuuac cggggaaaug aacaaagucg augagaauuu cuuucagaga      300

cucgacgauu ccuuccuggu ggacgaagau aagcggggag agcaccaucc gaucuuuggc      360

aacauugccg cugagguuaa auaccacgac gauuucccca caaucuauca ucugcgcaga      420

caccucgccg acaccucuaa gaaggcugau cugcggcuug uguaccuggc ccucgcgcau      480

augaucaaau uucgcggcca cuuccuguau gaaggggacc uuaaggccga gaauacggac      540

guccaggcuc uguuuaaaga uuucguggaa gaguacgaca agaccauuga ggaaagccac      600

cucuccgaga ucacaguuga ugcccugucu auucugaccg aaaaagugag caaguccagc      660

agacucgaga accugaucgc ucauuacccu acagagaaaa agaauacccu uuuuggaaac      720

cugauugccc ucucucugga ccuucacccc aauuucaaga cgaacuuuca gcuguccgaa      780

gaugcgaaac uccaauucag caaggacaca uaugaggaag aucuggaggg cuuccugggg      840

gaagucggag acgaguacgc cgaucucuuu gcuucugcca aaaaucugua ugacgcuauc      900

cuucuguccg gcauucucac cguggaugac aacagcacaa aggccccucu gucugccucc      960

auggugaaac gguacgagga acaucagaag gaccuuaaga aacugaagga uuucaucaaa     1020

gucaaugcuc ccgaccagua uaacgcgauc uuuaaggaua aaaacaagaa gggguacgcc     1080

agcuauauug aguccggagu uaaacaggac gaguucuaca aguaccucaa aggcauccug     1140

cugaagauua auggcucugg ggauuuucuc gacaaaaucg aucgcgaaga cuuccugaga     1200

aagcaacgga ccuuugauaa cggaagcauu ccucaccaga uccaccuuca ggaaaugcau     1260

gcuauccuga gacgccaggg cgagcacuau ccguuccuca aggaaaauca agacaaaauu     1320

gagaagaucc ugaccuuucg gauucccuac uauguggggc cucuggccag aaaaggaucc     1380

cgcuucgccu gggcugagua caaggccgau gaaaagauca cacccuggaa cuuugacgau     1440

auucuugaca aggagaaaag cgcggaaaag uucaucacgc ggaugacccu caaugaccug     1500

uaucugccug aggagaaggu gcuccccaaa cauucuccgc uguacgaggc uuuuacaguc     1560

uauaacgaac uuaccaaggu gaaauacguu aaugaacagg gcgaggccaa guucuucgau     1620

acaaacauga aacaggaaau cuuugaccac guguucaagg agaauagaaa ggucaccaaa     1680

gauaagcugc ucaacuaccu gaauaaagag uuugaagagu uccgcauugu gaaccugacg     1740

gggcuugaca aggaaaauaa agccuuuaac uccagccucg gaacauauca ugaucugcgg     1800

aagauccugg acaagucuuu ccucgaugac aaagcuaaug agaagaccau ugaagauauc     1860

auucaaaccc ugacacuuuu ugaggacaga gagaugaucc gccagcggcu gcagaaauac     1920

agcgauauuu ucaccaaggc ccagcucaaa aagcuggaaa gacgccacua uacaggcugg     1980

ggccggcugu ccuacaaacu uaucaacggg aucagaaaca aagagaauaa gaaaacgauu     2040

cucgacuauc ugaucgauga cggauacgcu aaccggaauu uuaugcaacu gauuaacgac     2100

gaugcccucu cuuucaagga agagaucgcg cgcgcccaga uuaucgacga uguugacgau     2160

aucgcuaaug ugguccacga ccugccuggc agccccgcca uuaaaaaggg gauccuucag     2220

uccgugaaaa uugucgauga acuggugaag guuaugggac auaacccugc uaauaucauu     2280

aucgagaugg ccagagagaa ccagaccaca gacaagggcc ggagaaauag ccaacagcgc     2340

cucaaacugc ugcaggauuc ucuuaagaac cucgacaauc ccgugaacau caaaaauguc     2400

gaaaaccagc aacugcagaa ugaucggcug uuucucuauu acauucagaa cgggaaggac     2460

auguacaccg gcgagacacu ggacaucaac aaucuuuccc aguaugauau ugaccacauc     2520

auuccccaag cguucaucaa agauaacagc cuggacaaua gagugcucac ccgcucugau     2580

aagaacagag gaaaguccga cgauguuccu agcaucgaag ugguccauga gaugaaaucc     2640

uuuuggucua agcugcugag cgugaaacuu auuacgcagc ggaaguucga caaucugacc     2700

aaagccgagc gcggcgggcu cacagaagag gauaaggcug gauucaucaa gagacagcug     2760

guggaaaccc ggcaaauuac aaaacacguu gcccagaucc ucgacgagcg cuuuaacacc     2820

gaauuugaug gcaauaagag acggauucgc aacgucaaaa ucauuacgcu gaaguccaau     2880

cuugugucua acuuuagaaa agaguucgag cuguacaagg ugcgggaaau caaugacuau     2940

caccaugcuc acgaugccua ccucaacgcc gucgugggga augcucugcu gcuuaaguau     3000

ccgcagcugg agcccgaauu uguuuacgga gaguauccua aauacaacag cuaccgcucc     3060

agaaagagcg cgacagaaaa auuccucuuu uauucuaaua uccugcgguu cuuuaagaaa     3120

gaggacauuc agaccaacga ggacggcgaa aucgccugga acaaggagaa gcauauuaaa     3180

auccucagaa aggugcuguc cuacccccaa gucaauauug ugaaaaagac agaggaacag     3240

accgggggcu ucagcaaaga aucuauccuu ccuaagggag aguccgauaa acugaucccc     3300

cgcaagacca agaacagcua uugggacccg aaaaaguacg gcggguuuga uucuccuguu     3360

guggcuuaua gcauucucgu cuucgccgac guggagaaag gaaaguccaa aaagcugcgg     3420

aagguucagg auaugguggg caucacaauu auggaaaaga agagauucga gaaaaauccc     3480

gucgacuuuc uggaacagcg cggguaccgg aacgugagac uugagaagau cauuaaacug     3540

ccuaaguaua gccucuucga gcuggaaaau aagcgccgga gacuccuggc cucugcuaaa     3600

gagcuucaaa agggaaacga acuggugauc ccccagcgcu uuacgacccu gcucuaccac     3660

uccuaccgga ucgagaaaga uuaugaaccu gagcauagag aguacgucga aaagcacaaa     3720

gacgaguuca aggaacugcu ugaguauauu agcguuuucu cccggaagua cgugcuggcc     3780

gauaauaacc ucacaaaaau cgaaaugcug uucucuaaga auaaagacgc ggaggugagc     3840

ucccucgcua agucuuuuau uagccugcuu accuucacag ccuuuggcgc ccccgcugcc     3900

uucaacuuuu ucggggagaa uaucgaucgc aaaagauaua ccuccgucac ggaaugccug     3960

aacgcgacac ugauucacca gucuaucacc ggccucuacg agacccggau ugaccugagc     4020

aaacuuggag aagauuga                                                   4038


<210>  4164
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4164
auggaccaga aguacagcau cggccuggau auugggacca acuccguggg augggccguc       60

guuacagacg auuauaaagu gcccgcuaag aaaaugaagg ugcucggcaa uacggacaaa      120

caaucuauca agaagaaccu gcuuggggcc cuucucuucg auagcggaga gaccgcggaa      180

gcuacaagac ugaaacggac cgcccgcaga cgguacacac gcagacggaa ucgccuuaga      240

uaucugcagg agauuuuugc cgaagagaug aacaaggucg acgaaaauuu cuuucagcgg      300

cucgaugacu ccuuccuggu ggaugaggac aaacgcggcg agagacaccc uaucuuuggg      360

aacauugcug ccgaaguuaa guaccaugau gacuucccga ccaucuauca ucugcggaaa      420

caccucgcug auaucucuca gaaggccgac cugcgccuug uguaccuggc gcucgcccac      480

augauuaagu uuagaggaca uuuccugauc gagggccaac uuaaagcuga aaauacaaac      540

guccaggccc uguuuaagga cuucguggag guuuaugaua aaaccgugga ggaaagccac      600

cucuccgaga ugacggucga cgcucugucu auucugaccg aaaaggugag caaaucccgg      660

cgccucgaga aucugaucgc ccacuacccc gcggaaaaga aaaacacacu uuuuggcaau      720

cugauugccc ucagccuggg gcuucagccu aacuucaaga ccaauuuuca gcugucugag      780

gaugcuaagc uccaauucuc caaagacaca uacgaggaag aucuggaggg acugcucggc      840

gaaaucgggg acgaguaugc cgaucuguuc gcuagcgcca agaaccuuua cgacgccauu      900

cugcucucug gaauccugac cguggaugac aauuccacga aagcuccccu uagcgcgucu      960

auggucaaga gauaugaaga acaucagaaa gaucugaaga agcucaaaga cuuuaucaag     1020

guuaacgccc cugaccagua caacgcuauu uucaaagaua agaauaaaaa gggcuaugcc     1080

ggguacaucg agaacggagu gaagcaggac gaauuuuaua aauaccugaa gggcauucug     1140

cuccaaauca auggcuccgg ggauuuccug gacaaaauug aucgggagga cuuucuuaga     1200

aagcagcgca cauucgauaa cggaagcauc ccccaccaga uccaucugca agaaaugcac     1260

gccauucucc ggagacagga ggaacacuac ccuuuucuga aagagaauca ggacaagauc     1320

gagaagauuc ugaccuuccg caucccguau uacgugggcc cccuugcucg gaaagggucc     1380

agauuugccu gggcggaaua uaaggcugau gagaaaauua caccuuggaa cuucgacgau     1440

auccucgaca aggaaaaauc ugccgagaag uuuaucaccc gcaugacccu gaaugaucug     1500

uaccuccccg aggaaaaagu ccugccuaag cauagccuuc uguaugagac auucacggug     1560

uacaacgaac ucaccaaggu uaaauaugug aaugagcagg gagaagccaa guuuuucgac     1620

gcuaacauga aacaagagau uuucgaccac gucuuuaagg agaaucggaa agugacaaag     1680

gauaagcugc ugaacuaccu uaauaaagag uucgaggaau uuagaaucgu uaaccucacc     1740

ggccuggaca aggagaauaa agccuucaac uccagccugg ggacauacca ugaucuccgc     1800

aagauucugg acaaaucuuu ucuugaugac aaggcuaacg aaaagaccau cgaggauauu     1860

auccagacgc ugacacucuu cgaggaccgg gaaaugauua gacagcgccu gcagaaauau     1920

uccgauaucu uuaccaaggc ccaacugaaa aagcuggagc ggagacacua caccggaugg     1980

ggccggcuca gcuauaaacu gaucaauggc auucgcaaca aggaaaauaa gaaaacaauc     2040

cuggacuacc ucauugauga cggguaugcg aacagaaauu ucaugcagcu gaucaacgau     2100

gacgcccuuu cuuuuaagga ggaaauugcu cgggcccaga ucaucggaga cguggaugac     2160

auugcuaaug ucgugcauga ucugcccggc agcccggcca ucaaaaaggg gauucuccag     2220

uccgucaaaa ucguggacga gcugguuaag gugaugggac acaacccugc gaauauuauc     2280

aucgagaugg ccagagaaaa ccaaaugacc gauaaaggcc gcagaaauuc ucagcagcgg     2340

cugaaacuuc uccaggacag ccugaagaac cuggauaauc ccgucaacau uaagaaugug     2400

gagaaccaac agcuccagaa cgaccgccug uuccuuuacu auauccaaaa ugggaaggau     2460

auguacacag gcgaaacgcu ggacauuaac aaucucuccc aguacgauau cgaccacauu     2520

aucccucagg cuuuuaucaa agacaacagc cuggauaaua gaguucugac ccggucugac     2580

aagaaccgcg gaaaauccga ugacgugccc agcauugagg ucgugcauga gaugaagucu     2640

uucuggucca aacuucugag cgugaagcuc aucacacaga gaaaguuuga uaaucugacc     2700

aaagccgaac ggggcgggcu cacagaggaa gacaaggcug gauucauuaa acgccaacug     2760

guugagacca gacagaucac gaagcacguc gcccagauuc uugaugaacg guucaacacc     2820

gaguuugacg gcaauaagcg cagaauccgg aacgugaaaa uuaucacacu gaaguccaau     2880

cucgugucua acuuccgcaa agaguuugaa cuguauaagg ucagagagau caaugauuac     2940

caucacgccc acgacgcuua ucugaacgcg gugguuggga augcccuucu gcucaaauac     3000

ccccagcugg aaccugaguu cguguaugga gaauacccga aguauaacag cuaccggucc     3060

agaaagucug cuaccgagaa auuucucuuc uacagcaaca uucugcgcuu uuucaagaaa     3120

gaggauaucc aaacaaauga agacggcgag auugccugga acaaggaaaa acauaucaag     3180

auucuucgga agguccuguc cuauccccag gugaauaucg uuaaaaagac cgaggaacag     3240

acagggggcu uuagcaaaga gucuauccuc ccuaagggag aguccgauaa acugauuccc     3300

agaaagacga aaaacagcua cuggaauccu aagaaguaug gcggguucga cucucccgug     3360

gucgccuacu ccauccuggu guuugcugac guugaaaaag gaaagagcaa aaagcugcgc     3420

aaagugcagg auauggucgg cauuaccauc auggagaaga agcgguucga aaaacacccg     3480

guggacuucc uugagcaaag aggguaucgc aacgugcggc ucgagaagau uaucaaacug     3540

ccuaaguacu cucucuuuga acuggagaau aaaagacgcc ggcuucuggc cagcgcgaga     3600

gaacugcaga agggaaacga gcucgucauc ccccagcggu ucacaacccu gcuuuaucau     3660

uccuaccaaa uugaaaagaa uuacgagccu gagcaccgcg aauauguuga gaaacauaag     3720

gaugaauuua aagagcugcu cgaauacauc agcguguucu cuagaaagua ugugcuggcu     3780

gacaacaauc ucaccaaaau ugagaugcug uuuuccaaga acaaggaugc cgaggucagc     3840

ucccuugcca aaucuuucau cagccugcug acauuuacgg cuuucggcgc ccccgcggcu     3900

uuuaauuucu uuggggaaaa cauugaccgg aagcgcuaca ccuccgugac agagugccuc     3960

aaugccaccc ugauccacca gucuauuaca ggccuuuaug aaaccagaau cgaucugagc     4020

aaacucggag aggacuga                                                   4038


<210>  4165
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4165
augaagaaag aguacagcau cggccuggac auugggacca acuccguggg augggccguc       60

aucacagaug acuauaaggu ucccgcuaaa aagaugaaag ugcucggcaa uacggauaag      120

cagucuauua agaaaaaccu gcuuggggcc cugcucuuug acagcggaga aaccgcggag      180

gcuacaagac ugaagcggac cgcccgcaga cgguacacac gccggaaaaa uagacuucgc      240

uaucugcaag aaaucuuuac cgaggaaaug aacaaggugg augagaauuu cuuucagaga      300

cucgacgauu ccuuccuggu cgaggaagac aaacagggcu cuaaguaccc uauuuuuggg      360

acacugaagg aggaaaaaga guaucacaag aaauucaaga ccaucuacca ucuccgggag      420

gaacuggcca acagcaaaga gaaggcugau cuucgccugg uguaucucgc ccuggcucac      480

augaucaaau uuagaggaca uuuccuuuac gaaggcgacc ugaaggccga gaauacggau      540

guucaggcgc ucuuuaagga cuucguggaa gaguacgaua aaaccauuga ggaaucccac      600

cugucugaga ucacagucga cgcccugagc auucucaccg aaaagguguc caaaagcucu      660

cggcuggaga accuuaucgc ucauuauccg acagaaaaga aaaauacccu guuuggcaac      720

cucauugccc ugucccuugg gcugcaaccc aauuucaaga cgaacuuuca gcucagcgag      780

gacgcuaagc ugcaguucuc uaaagauaca uacgaggaag accuggagga acuccuggga      840

gagaucggcg augaauaugc cgaccuuuuc gcguccgcca agaaucugua cgaugcuauu      900

cuccugagcg ggauccuugc cguugacgau aacaccacaa aagcuccucu gucugccucc      960

auggugaagc gcuaugagga gcaccagaaa gaccucaaga agcugaaaga uuuuaucaag     1020

gucaaugccc ccgaccaaua caacgcuauu uucaaagaua agaacaaaaa gggauaugcg     1080

ggcuacaucg aaagcggggu ggagcaggac gaauuuuaca aguaucugaa aggcauucuc     1140

cugaagauca auggcuccgg cgacuuccuu gauaaaauug acugcgagga uuuucugaga     1200

aagcagcgga ccuucgacaa cgggucuauc ccucaccaga uccaucucca agagaugcac     1260

gccauucugc gcagacaggg agaacauuac cccuuucuga aagagaauca ggauaagauc     1320

gagaaaauuc uuaccuuccg gaucccuuau uacgugggac cgcucgcuag aaaggggagc     1380

cgcuucgccu gggccgaaua uaaggcugac gagaaaauua cacccuggaa cuucgaugac     1440

auccuggaua aggaaaaauc cgccgagaag uuuaucacgc ggaugacccu gaaugaccuc     1500

uaccugccug aagagaaagu ccuucccaag cacagcccuc uguaugaaac auucaccguu     1560

uacaacgagc ucacaaaggu gaaauacgug aaugaacaag gagaggcgaa guuuuucgau     1620

accaacauga aacaggagau uuucgaccac gucuuuaagg aaaauagaaa agugacgaag     1680

gauaagcugc ugaacuaucu uaauaaagag uucgaagagu uucgcaucgu uaaccucaca     1740

ggccuggaca aggaaaauaa agccuucaac ucuucccugg ggaccuacca ugaccuccgg     1800

aagauucugg auaaaucuuu ucuugacgau aaggcuaaug agaagaccau cgaggacauu     1860

auccagacac ugacccucuu cgaagauaga gagaugauuc gccagcggcu gcaaaaauau     1920

agcgacaucu uuacaaaggc ccagcugaag aaacuugaaa gacgccacua cacgggaugg     1980

ggccggcuca gcuauaaacu gaucaacggc auuagaaaca aggagaauaa aaagaccauc     2040

cuggauuacc ucauugacga uggguaugcu aaccgcaauu ucaugcagcu gaucaacgac     2100

gaugcccuuu ccuuuaagga ggaaauugcu cgggcccaga ucaucggaga cguggaugac     2160

auugcgaaug ucgugcauga ccugcccggc ucuccggcca ucaaaaaggg gauucuccaa     2220

agcguuaaaa ucguggauga gcuggucaag gugaugggac acaacccugc uaauauuauc     2280

aucgaaaugg ccagagagaa ccagacaacc gacaaaggcc ggcgcaauuc ccagcagaga     2340

cugaaacuuc uccaagauag ccugaagaac cuggacaauc ccgucaacau uaagaaugug     2400

gaaaaccagc agcuccagaa ugaucggcug uuccuuuacu acauccaaaa cgggaaggac     2460

auguauacag gcgagacccu ggauauuaac aaucucucuc aguacgacau cgaucauauu     2520

aucccucagg cuuuuaucaa agacaacucc cuggauaaua gaguucugac gcgcagcgac     2580

aagaaccggg gaaaaucuga cgaugugccc uccauugagg ucgugcacga aaugaaaucc     2640

uucuggagca agcugcuuuc uguuaagcuc aucacccaaa gaaaguuuga caaucugaca     2700

aaagccgagc gcggcgggcu caccgaagag gauaaggcgg gauucauuaa aagacagcug     2760

guggaaacac ggcagaucac caagcacguc gcccagauuc uugacgagcg cuucaacacg     2820

gaguuugaug gcgcucaaag acggauccgc aaugugaaga uuaucacacu gaaaagcaac     2880

cucgugucca auuucagaaa ggaauuugag cuguauaaag uucgggaaau caacgacuac     2940

caucacgccc augaugcuua ucugaaugcc gucgugggga acgcccuucu gcucaaguac     3000

ccccagcugg agccugaguu cguguaugga gaauacccga aauacaauuc uuaucgcagc     3060

agaaaguccg cuaccgagaa guuucucuuc uacagcaaca uucugcgguu uuucaaaaag     3120

gaagacaucc agacaaauga ggauggcgaa auugcgugga acaaagagaa gcacaucaaa     3180

auucuucgca agguccuguc uuauccccag gugaacaucg uuaaaaagac cgaggaacaa     3240

accgggggcu uuuccaagga gagcauccuc ccuaaaggag aaucugacaa gcugauuccc     3300

agaaaaacga aaaauuccua cuggaacccu aagaaguaug gcggguucga uagccccgug     3360

gucgccuacu cuauccuggu guuugcugac guugagaagg gaaaaagcaa gaaacuucgg     3420

aaggugcagg auauggucgg cauuacaauc auggagaaaa agagauucga aaagaauccg     3480

guggacuucc uggagcagcg cggguaucgg aacguuagac ucgagaaaau uaucaagcug     3540

ccuaaauacu cccucuuuga acuggaaaau aagcgccgga gacuucuggc cagcgcccgc     3600

gagcugcaga aaggaaacga acucgugauc ccccaacggu ucaccacacu gcuuuaccac     3660

ucuuaucaga uugagaagaa uuacgagccu gaacauagag aguaugucga aaagcacaaa     3720

gacgaguuua aggaacugcu cgaguacauc uccguguuca gccgcaaaua ugugcuggcu     3780

gauaacaauc ucaccaagau ugagaugcug uuuuccaaaa acaaggacgc cgaagucucu     3840

agccuugcga aauccuucau cucucugcug acauuuaccg cuuucggcgc ccccgccgcu     3900

uuuaauuucu uuggggagaa cauugaucgg aagagauaca cgagcguuac agaaugucuc     3960

aaugccaccc ugauccauca guccauuacc ggccuuuaug agacacggau cgaccugucu     4020

aagcucggag aggauuga                                                   4038


<210>  4166
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4166
augaaccaga ccaagaauua cgacaucggc cuggauauug gggugggaag cgucggcugg       60

acaaucacgg acucccaauc ucagaaaguu cucaagguga aagggaagca gggaauuggc      120

gugcaccugu ucaacgaggg gcagaccgcc gaagagagac ggcaauuucg cacaaauaga      180

cggcgccuua aaagacggaa guggcgccug agacuccugc gggaacuuuu cgaugagccc      240

aucagcucca uugacccuga auuuuucgcu cgccugaagu auuuuaacga gucuagcaaa      300

gauccgaaga auaaaggaua ccagccccag cuccaugacg aucugaagga cgucgauuuc      360

uauagacagu acccuaccau cuaucaucug cggcacgccc ucaugacaca aaaagagaag      420

uuugacaucc gcgauauuua ccuggcgauc caccauauug ugaaguauag aggccacuuc      480

cuuaugaacg gccacuccuc uagcuuuucc guugcucaga ccgacuggaa agccgaucug      540

acaaaacucu ucgaacugau ccaggagacc cggacgaauc uugacuuuag cuucgaccug      600

accgaaauug auacaaucaa aucucuccug uuugacaaga accugucccg caaugauaga      660

cagaaagaga uuguggggcu caucaacaau aacagccaac uggacaagaa agauaaggcc      720

cuuaccaagc agcuggucga gucugugguu aaaggaaucg ugggcaugaa gacacggacc      780

gacauucucc ugggguacga agucgauaau aaagagcagu ccaaggcuug gaacuucagc      840

aucgacgaac uugaugacca aauggccacg auugaucccg cucugucuca aaauucccag      900

gcccaugaca ucauuacacu cauccagaaa cuguacauga ccgugcagcu ggcgcagauc      960

gugccugagg gacagagcgu cuccgaaaug augauuaaca gauauaaccu cuacgccgag     1020

cagcugcaac agcuuaagga cuauaagaaa aucaaucugg agacaggccu ccugcuucgc     1080

gaagccuacg augagcugau gaccaaggcc cucaccgcug cccccgggaa aacaauucac     1140

gacacguucu auaacaaggu uaaaaagauc cugaaggauc aggacaauaa acaggcgcug     1200

ucuauuaagc aagccaucga agauagcaac uaccucaucc cucugcggau uaaauccaau     1260

ggagugaucc cguaucagcu ucaucagaga gagcuggacg cuauuaucga aaaccagaag     1320

gaguuuuacc ccuuccucgc caccccuaau cccguggaug gccaccaaga ggcucugccu     1380

uacaaacugg acgaacuuau uggcuuuaag guccccuauu acguggggcc gaugguugau     1440

ccugaggugg acagcgauca gaacuucgcc uggauggucc gcaaggaaaa aggagagauc     1500

acacccugga auuuucacaa caagguggac auuacccagu cugccgauaa uuucaucaaa     1560

cggaugaaga caaccgacac guaucucauc ggcaaagacg uucugccuca aaacucccug     1620

cucuaccaga gauauacagu gcugaaugaa cuuaacggga uuaaggucgc uaauaaaaag     1680

cugaccaucg agcagaagca gcgccucuac gaucaacugu uuaaaaagaa aaagaccgug     1740

aaaaagcagg acauucugaa caaccuuguc gcggccggag agauccauga agaugacaau     1800

cucacaggcc ugagcaacgg gaccacauuc aauaacaauc ugggaucuua uauugauuac     1860

ugcaagauca ucggccagga caaacucgag gaugcuagca agcaggccga cauugaacgg     1920

aucauugagu gguccacgac cuuugaggau gccaacaucu uccugacaaa acuugcugaa     1980

auuaauuggc ugaccgagga ccaaagacag aagcucucuc agauccgcua ccggggcugg     2040

gggcaguuua gcaaaacacu gcugaccgau aucaaggaca acaaggauaa uuccauuauc     2100

gacaaccuua ugagcacgaa aaagguguuc augcaaauua ucaaugacac cacaauuaaa     2160

cagaagaucg augacuauaa ccaguaccac cucaaucaug ccgaucugaa agacaccauc     2220

gcgaaccugu uuacaucucc ccaaaauaag agagcuauua accagacccu ccugguuauc     2280

gaagauauua ucaacgccgu gggacacaag cccaaucgca uuuucaucga guccgccaaa     2340

gacaaccgga augcuaagaa ccagcuuacg cagagcagac ugaaacggau ugucaagcaa     2400

uucgaagaua auaaacagca uguggaccag ucuguuuaug aggagcucaa gcagcuggau     2460

gacggcuccc acgccagcau cgaugaccgc cugcuucucu acuuucugca aaacgggaaa     2520

gacauguaua caggacagcc uaucgauauu ucccagcugu cuaccagcca guaccacauc     2580

gaccauauuc ucccgcaagc uaaggugaug gauaaugccc uggacaacag aguccuugug     2640

uccucuaagg cgaauacaga aaagagcgau cgguuccugu ccgagauccc ccaguuucag     2700

cagagaagcc ucugggccga acuggagcgc augggcuucc ugaccaaggc uaaaaaggaa     2760

cggcuucuga ugaccucuga cgaguuuaaa caaaacuccg ugagauucau ugcccgccag     2820

cucguugaga caagacagau caucaagcug gucaaucaac ucauuaucca ggaacacccu     2880

gaggugaagc uggugaacau uaaagcugau cuuacgaaug ccuuucggaa gaaauucaac     2940

uuugucaaga aucgcaacgc gaacgacuau caucacgccu ucgaugcuua ccugaccagc     3000

cucaucgggu cucaucugcu gaaaucccac cccaaguggg aaccucuuuu uguguacagc     3060

gacuaugaua agauuaaauu cgacaaugcu ccccagggcg uuaacaucau gaaggaguuu     3120

gaacagucua aacuggcuga ugagaaugga gagaucauuu ggaacaagga cgaaaugaug     3180

gccuaccucg agagacugua uaauagccgg caaauccuca uuacaaagaa gguguacgcc     3240

cgccacggca accuguucaa gcagaccauc uucaaagcug gggaauccga caaucuuauu     3300

ccggucaaga acaauagaag cgauaucgcg auuuauggag gcuacaaauc uaaacaggug     3360

uccuauuacg cccugaucag ccuccauaac aaaaagcggc agaagcgccu gguugcugug     3420

uaccugaucg acauucaaaa acuucagaau cugaacgagu ccgauuauuu uaagacacuc     3480

cacaccauca uuaaagagag acauaauauc aagccugagu ucgacauucu ggccgauaaa     3540

aucuaccaga accagcucau cauugacaau aagaacgggu cuaaucggcc cuuucgcauc     3600

gaaggagauu ucaaaaacga cagacaacug uaucuuccuc ugcaguacca gcgguggcug     3660

gccagcaagg aacccacgga ugagaagcuc aacagaauuu uugaccacau cugugaacaa     3720

cuggcugauu auuucccucu uuuugccggc aaugcgugcg acaaacugcu cgauaaccgc     3780

gacaaguucu accagcuguc cauuaaaucu gaguuuaaug acaaggggaa acucaucgcu     3840

gagggcaagc ugagcgucau cgauaaacuu cugguggcca ugcacgccga cucccggaac     3900

ucuaaucugg cuguuauugg acucagcucc agcuggggca gauucaagcu gcauggggga     3960

aacgaagcca ucggcacauc ugugcagcuu auucaccagu cccccaccgg gcuguuuaag     4020

cgcauccaac gguucaauug a                                               4041


<210>  4167
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4167
augcugagcg ccggcgugau gcucggggac gucuccgcuc ugaagucuag cagaaacucc       60

cuuuuccaga aaucuaagaa acugaucgag aauagcuccu cugaucucac caagguugac      120

uacagcaaaa ugcgggaaau ucugcuuucc auccgcaaca uugccaucag auuuuucaau      180

aacgaggauu uuaaggaaag cgacuauuac aaggaguucc ccacaauuug gcaucugcgg      240

aaaaaucuca uggaaucuga uaagaaagcg gacauccgcc ugguguaucu ggcucuccac      300

aacaucguga aguacagagg aaauuuucug caagagaaca auaaagaucu ucgggccgcc      360

aacgcuaaug ccggcgcugc cgcgauugac cuguccaacg cccucgagga auauuucagc      420

cugcacgagg augaaucugc ucuuacgugc gucccuaaug uggccgaccu gcagaugacc      480

cucgagcagc cggggcugcg caacggagag agacuggauc ggaucguugc ugcccucaau      540

augcccgacc gcgcgagagc caagcuguuu gcuaaggccu guauuggcca gaaaguggag      600

uucuccaagc uggcucuggg gauggagaaa caagaaggaa caaacgccua caucugggau      660

gacgacaagu augaugagcu cgucgcccug ugcccugaag acuuuuuccc cgugcuugag      720

acccugaaaa acgcuuacag cgcguacguu cucucugauc ugcuggccgg caagcucucc      780

auuagcgcuu cccugaucga cucuuaugag cagcaucaga augaucuuaa aauugugaag      840

gaacuggucc gggaguaccu cggccagaag gaauaugggc gccuguuuag aggaccuaaa      900

uucgccaacg gcgacuacaa uuuuaacaag guggccaaac ccagcuauac auccuacauc      960

cuuggggaga gccuguguaa uaagcaagga accacacggg aagaacucgu gaaagagcug     1020

aagcgcgucu ucgaugacug cucugcuauc caggccgaua agagauauca gaaaauuaag     1080

cagcggcugg aagacgaucc uaaauuccuc aucaagcaaa agaccagagc gaacggcgcu     1140

auuccguacc agcugcaccu ugaggaaaug caucagauca uugagaagca agggaaguac     1200

uaucccuuuc ugguugaaca gcaggaccuc aucgagaaac ugguguccag ccgcaucccu     1260

uacuaugugg gaccccugaa uggcggccac gacccuaacc accccuaccc gacgaauccu     1320

auugauccca agcggaaauu cgccuggucu gucagaaagc cugggaugga gcaugaaaaa     1380

gccuaucccu ggaacgugga cgagguuauc gauagcgaag agaccgcuga gcgcuuuauu     1440

cggagaauga caggaaccug uacauaccuu uauggcgaac ccgugguccc ucgcugcucc     1500

cuccuguacg aggaguucug ugugcugaau gaacucaacg gggcccggug gggagaaggc     1560

aauggggaca cccacagauu ugauucuagc gaccgcacgg agauccugga gcagcuuuuc     1620

aagaagcgga aaagaguuac acauaaggcu auugccaccu ggcuggaaau caaccacgga     1680

gugacaaaug ucaaaaucuc cggcacccaa gcggagaacg ccuuugaaag caagcucucu     1740

uccuacaacg auuucugcaa ucugcugggc guggacgggc uugaggaugu cuguccgcuc     1800

accaaagacg auauugaaca gaucauucug ugggugacag uuuuugagga caagaaacug     1860

cuccugcaga aacuucagcg cgcuuucgga gauaagcuga gcgagaaaca aaucgccucu     1920

auuaugcgga agcccuaugc uggcuggggg agacucucca gcaaacugcu gacgggaauc     1980

aagcgggaaa ccccuuuugg ccgcauuucc auccuugacg ugaugagaga cggggauccc     2040

cggccuggcc accaucucga cgccaugaac uucauggaga uccugagaga aaaagauuuu     2100

ggauuccagg accgcauuga ugagaucaau aaggaacgga caaaggaguc uggccugcuc     2160

cugagcgucg aggaccuucc cggguccccg gcgcugagac gcucugugaa ccaggccaug     2220

cacauucuca aagaaaucgu uagcauugcu ggaaaggagc cuuccagaau cgugaucgaa     2280

gucacccggg aucccgacga ggagaaacgc ggccauagaa cagauacccg guacaagcag     2340

cugagcgaag cccuggcugc cauuaaaacg gacaucgugc aauucgauuc uucccuucuc     2400

gccgagcuga aggaacgcaa ggacagccug gauucugaga gacucaugcu guauuuucag     2460

cagugcggga aaucccuuua cagcggaaag ccucuggaca uuaaucggcu cucugaauau     2520

cagguggacc acaucauucc ccaauguuac aucaaagaug acagccugga uaacaaggcu     2580

cugguuuucg cguccgagaa ucagaucaaa cuugacagcc ugcuccugga uaagucuauu     2640

auccgcuccc agaagggcag auggcaagag cuccaucggg ccgggcugau uagcaacaaa     2700

aaguuucgca aucuuacaug caccgaaauc uccgacagaa ugaugaaagg cuucauuaac     2760

cggcagcugg ucgagaccuc ucagaucgug aaguuuguga gacagaugug cgaacaacgc     2820

uaugagagcu ccgaagucau cucucuccgg gcuagccugu cccacaaucu gagagaucgc     2880

uguggauuca uuaaaugccg ggagcuuaac gacuaccacc augcccacga ugccuaccug     2940

gcuugucaga ucgccagauu uauuggcauc cgcuauccuc gguggcagga cgggauuucu     3000

agagaguuca ucaauaagua cauuaaacag cucgugaaga agggaaaaag cuccggcaag     3060

uuuuucgcgg ggaaaagcgg auuuaucauc gauucuuuca ugcaugacgg cuuugaccgc     3120

gaaacagggg agauuuuccg ggauggcugg gacgcuaccu ucgaaaucac aagaaugcgg     3180

cgcguucugg gauauaagga uugcuuuauu acgagaaugc ccgaggaaca aaccggcgcc     3240

uacugggagg agacaaucua uuccccgcuc gacgccaaaa acgggaagaa ucugcagauu     3300

ccucuuaagc agagcggaaa agaaggcuac cuggauccca agaaauaugg gggaaccaac     3360

acagcuuuca acgccuacuu uuucgcguuu guggcuaagg acgcccgggg ccgcgauaaa     3420

uacuucuuug aggggguccc cguguaucuc gccaagcagc ugaaugaaca accucagauc     3480

cugguugacu ucgcuaacuc uaucgccgcu accaagaaau guuccacggc caagauucuu     3540

agaagcaaag ugccgcugcg gcagaaguuu cuccuggaug gcaccucuuu cuaccucgga     3600

ggcagaacag ggaauaaaaa cgucauccug uccggagugg aguuugcgug cggcauugac     3660

auggccaaga ugaucgauac cgcuauuaag caccccgagg aacuuagcca aaaagagcug     3720

aucuccauca uucagaugcu ggccgacaca aucgaacgca auagcgaucg gcaggcuucu     3780

cucauuaacu ucagacgcuu cgacgacaag cugcggaaua ucgaugccau ugccgcuagc     3840

agaaucauua aaaaccuucu uaauuccaug aacgggguua agcagucuug ugaccucagc     3900

gaucugggag gcuccaaaca aucuggcuuu augcagagca auaucgcgac ccucuucgag     3960

cgcaucacgu ggugcuauuc cucuauuaca gggauguuug aacagcgggu gagcuucgag     4020

gaccugaaga acggaaucug a                                               4041


<210>  4168
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4168
augaagaaaa gaaaccggga ggaauacuau gugggccugg acaucgggac cagcuccguc       60

ggaugggccg uugcuacacc cgauuaccgc auuccuaagu ucagaggcca caaaaugugg      120

gggucucggc ucuuugccga gggaaagcgc gcggcugaaa gacggcaguu ccgcacgaau      180

agacggcgcc uggccagacg gcgcgacaga cuucaaaucc uggaggaacu cuuugccgag      240

gagauggcua aaguggauaa caccuucuuu augcggcuga aggaaagcaa guucuuucuu      300

gaggacaaaa cagugaagga aaaacauauu cuguucucca ccgauaaguu ugacgccgau      360

cgcuucuaua aagacaaucc gacaaucuac cacgucagaa agcggcucau ggaggaggaa      420

ccccaggaua uuagagagcu guuucuggcu gugcaucaca uccucaagua ucgcggccau      480

uuccuguuug ccgggucucu ugaacagaac ggagacagca ucgaggaacu gguucggacc      540

cucuucggca aacagggccu ggcgauucuu gaugacacaa ucgauuccaa ugagauggug      600

aacauucuga agaauaacga gaaaaccaag ucugacagaa agaaggccgu ccaagaacuc      660

uggaauccuc ugaaagacaa ggugaagaaa gcucaggccg cugagauggc cgggguugug      720

cugggacuca acagcgauau cuccaagcug uucgacaaua gcgauuacaa agaccuuaag      780

auuaacuuuu cuuccggcga uuaugacgaa aaacgcgaug acuacgcgaa uagcaucggg      840

gaugacauug uccugcucga uacgaugaag gcccuguacg acacccuuau ccugucucag      900

auccuccagg gaaagcaauc cauuagcgag gcuaaagugg aagacuauaa cacacacaag      960

gagcagcugc gggcccugaa agcuauccuc aagccccaca aaagacugua caaugagaug     1020

uuccgcgaaa acaacaagaa ggaucuuucu aauuaugugg ccuacauuuc cagcuauucc     1080

gagggcaaac gggucaacag agaaggguuu uacaaguaug uucaggccgu gcugaaacag     1140

guggacgauu cugacagcaa gcaagcuauc cucgaggcga uucgccugga aaccuuccuu     1200

ccucugcucc gggucaaaga uaaugccgug aucccccauc agauccacca ggcugagcug     1260

gaggccauuc uggaaacagc cagaaagaag uacaaauuuc ucaacgacgu uuccgaggga     1320

gugagcguca aggauaaaau cauucaacug uucaccuuuc gcaucccuua cuaugugggc     1380

ccgcuuaauc cccauucugg cgaaacgguu gcugaccgga agcaggggac aggaugggug     1440

gucagaaaac agcggggcaa gguguacccu uggaacuucg ccgauauggu cgacgugaaa     1500

gcguccgcug aggccuuuau uaccaaucug acaaacaagu gcaccuaucu caucggggag     1560

gauguucugc ccaagcgcag ccugcuuuac ucugaguuca ugcuccugaa ugagcugaac     1620

augaucaaau augacggaca cgaacucccu guggagauua agagacugua caucgaacag     1680

gucuuuaaac aagagcacaa gaaacagacc aagggccggg ugcuugauuu ccugaagaga     1740

aaugggcuca uuacaggaag cgccacggcu guuaucaccg gcauggacga cuccauuaaa     1800

ggcgaucugc agucuuaucg cgacauggug cggaucuuug ggacaggauu cgaucauagc     1860

auggccgaga gaauuauccg cugggucacc cuguucggcg aagcuaagga cauccuugag     1920

aacaaaauua gagaagugua cggaaauucc aucagcgauu cucagaugcg ggccauuaag     1980

aaacucaagu acgcggacug ggggcgccug ucccaagccu uuuucaucaa gauuaucggc     2040

ugugauaaaa gcggggugga gagaaacauc auuacaugcc ugcgggaauc uucccagaau     2100

cucauggaga uccugagcga ccagggcacc uauauuaagc agaucaaggc uuggaaccac     2160

caacauacga caaacaugau ggauacccuu gacuaccagc ugguugauga ccucuauguc     2220

gcccccuccg ugaaacgcuc uauuuggcag agccugugua ucguggagga acuggucaaa     2280

aucaugggac aggcuccgaa gaagauuuuu guggagguua ccagaacaaa ucaagccgag     2340

aaaaccaaga aagauucccg gaagaaacgc cuuguggaac ucuacacagc gaucggcaag     2400

gaaggggccg cuuggaaaaa ggagauugag aagcaccagg ccggagacuu cagacggaaa     2460

aagcuguauc uguacuauac gcagaugggc cgcugcaugu acucucauga accuaucgcu     2520

auggacgccc ucuuuaccag cgaguacgau auugaccaca ucuaucccca aucccugaca     2580

aaagaugaca gcugggauaa ccuuguccug gugaagucuc agcucaauaa agaaaagucc     2640

gaccaguacc cucugagacc cgccauccgg gagaagaaca aagcguauug gcaguaccug     2700

uguagagcug gguucauuag cgagaagaaa uaugaacgcc uuaugcggac cacagagcuc     2760

accgccgaag aacugucugg auuuaucgcu agacaacugg uugaaacguc ccaggccacc     2820

aaggccguga uuggccuccu ggagcgcauc uacaaacaca cagauauuug cuucgucaag     2880

gcugagaccg ugagccaguu ucggcaucag cacaauuuca ucaagauuag agaacuuaac     2940

aauuaucauc acgccaaaga cgcguaccug aacaucguug uggggaaugu cuaccacgag     3000

aaguuuacca aaaacccccg cgaguucauc gcucggucua cagaaagacg cccuuauaau     3060

cucaaccgga uguacgauuu ugaccugcug aagcaaggca gagugauuug gcaagugccg     3120

aaaagccuug aaacggucca ggccaugaug aaguccaaug auguucggau cacaaagaaa     3180

cugcucgugc agaagggaga gcuguaugac gccaccauua caaaagcuca uaagaccaaa     3240

gagggcaugu acuucccccu caagggggcc gacgcgcgcc ugagagaugu gcaccgguau     3300

ggaggcuacg aaaagaucaa aauugcuuau uacagcgucu ucucuuguug cgagaaggcc     3360

uccgacacca uccauacacu uaucauuccu auccccgugu acgccgcugg gcugcuccag     3420

acggaagccg aucugaccgc uuaugccugg cagacaaaac agaagaccaa accuauuaag     3480

gacaucuaca gcaugauuac acccaucuau aaaaagcugu guaucaacac cguugugaag     3540

auugauggau uuaaauacuu ccuuggcggg cgcacggagg acuauaucug ccuguacccg     3600

gcgauuccug ucgugcucga ugaacaagcc gcugccuauc ugaaggcugu uuccaaagug     3660

ggcagagccg acgaugucga gaauacagcc caggcuaucg accucauuac cgcggaggcc     3720

aacgaggcuu uuuacgaugu gcugcuuaag aaaauggaag ccaagaucuu cguuaaggcc     3780

uaccaggaac ggguggagaa acuggcuucu gagaccgcca gagaacgcuu ucggaagcuc     3840

agcauuggag cgcaguguuc cugccugcug gagauccuua accugcucgc uaauaacaaa     3900

acaaccucua aucugaagaa auggcucggc gucacaauga gcagacgcaa gcaacccaug     3960

gaccugacga aacuugaaga gugcaucgug uaugaaaccu ccauuacagg gcuguacgag     4020

aaagccgugu cucugcucug a                                               4041


<210>  4169
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4169
augguggagc acuacgccau cggcuucgac auugggaccg gaagcacagg cuuugcuggg       60

gucgauccca acggaaagcu gguuagaaug cggggcaaaa auguguaugg gguguaccgc      120

uucaagggag gcgaagccgc ggcugacaga cggggcuuuc gcacgaccag acggcgcauc      180

aaaagacgga aguggcgccu cagauuccug aaauccuuuu ucgagccuua uauugccccg      240

aucgaucccg aauuuuuccu ucggcagaag uacucugccg acagcccucu ggaucccaag      300

gggauucgcg cugacaaagg acuccugucc gauaugucug accaagaguu ucaugccaag      360

uauccuacaa ucuaccaccu uagauaucag cugaugcaug aaaaacggca guucgauguc      420

cgcgagaucu accucgcuau gcaccauauu aucaaguaca gaggccacuu ucugauugag      480

gacggggccg auaacuucga cgcgaaacag aucgauuucu ucgaccaauu ccagcggaug      540

gccgcuguga ccgccggacu gcccgaacuc gagacacugg uugugccuga cgaaacccag      600

agcgaugcuu uuaagagagu ccuuuucgac gagucccaga caaagucuga ucgccaaaaa      660

gugcuggcca gccagcuccu ggacauucag gauaaucagu ccaaggcgau caaagacuuu      720

auugccgcuc uuaccaaggc cguugugggc augaaagcua accuggccac gcucaccagc      780

acagccaucg agucuaccga uaagaaugac uggacaaaac cgcuggggca acuggaugac      840

uuccuccuga agcacgaaga ggaucuugac gaagacggau augcucugau cgaugcggcc      900

aaccagaugu acuccgcugc cguccucgcc gcucuggugc ccgagggcau gggcuuuagc      960

gaagccauga uggcgcggua ugcucagcau aagaaacaac uugcccugua caaacaggug     1020

auugccucuc ucccugacaa gacccagaga cacgagcuga aggaugcuua ugccgacuac     1080

aucgagcaug cugcccagaa uccccgcgau gcguucuaua aacgggucac gaagguucug     1140

gaaggggugg acuccgccga ugcuagaaca gugaugaccg ccauuagcca cgacgaguuu     1200

cuccugaagc aacgcacacu uucuaacgga aaaaucccuu accagaugca gcagcacgaa     1260

uuugaugcua uuaucgagaa ucaaaaggcc uacuaucccu ggcuggcgac cccgaacccu     1320

gucgaagccg acgcuaaacg gcuccccuac uaucuggaug agcuuaucgg cuuuagagug     1380

ccuuacuaug uugggcccau ggugaccgag aagcaguccc aguucgccug gaugauucgc     1440

aaagcugacg gagaacugac acccuggaau uucuucgaug ucguggaccg ggagaagacg     1500

gccaacagau uuauccagcg caugcaaacc acagacaagu acauucucgg cgaagauguu     1560

cugccuaaac auagcauccu guaucagcag uucgaggugc ucaaugaacu gaacaauauu     1620

cgggucgaga agcacccgcu uaccacagag cagaaacaau ucaucuacca gagacuguuu     1680

guggaccagc aacggaagac caucacgaaa aagaaccucc aggauacacu ggucgccaug     1740

ggggaauuug acaaggcucc cgauauugga ggccuggcga aagaggacgu gaugaugucc     1800

ucucuugcca ccuuugcuga uuacagcaag auccucccug aagccauuuc cgaccccacc     1860

cgccagaacg auaucgagga cauuaucgcc uggagcacaa uuuucgagga uacccagauc     1920

uuuuucgaca aacugaagac aguugauugg cugacgaccc aacagagaaa acggcucgcu     1980

ucuaucagau auacaggcug ggggaagcug uccaaacgcc uucugcagca gauuccuacc     2040

agcaagcaua caaaugugau ggccgcgcuc ugggcuaccu cugacaacuu uaugcaaauc     2100

cugcaggacg aacaguucca ggagggacug uacgaugcca aucggcuucc cgaagagacg     2160

cucaccccgg acgaacaagu cgagaagauu cuggaugagg ccuaucugag cccugcugac     2220

aaaaagggcg ccagaaaagu gaucaagguu augaacgaua uugugcgcgc gaaucacggg     2280

guccuccccg aacagguguu ucuggagucc gcugacgauc cucagaaaaa caagagacgg     2340

ucugugagcc gcuccaagca acuugaagag aaauacagac aggccgccca gagcuucauc     2400

gacgaagcug uuaaggagga gcugaaugcc cagaucguca acucugcuga auuuaaugau     2460

cggcucgugc uguauuucac acaaaacgga cgcgacaugu acaccggcaa aagacuggau     2520

auugagaauc uuggguccua ucagaucgac cauauucucc cccagagccu ggugaaggac     2580

gauucucugg acaaccgggc ccucacagcg cgcgauauca augccgaaau guccaaccag     2640

uuugucagcg agagauucgg ccugggaccu cgggcuuucu ggcaacagcu uaaaaauaac     2700

ggccugauuu ccgggcgcaa guuuagaaac cucgugaugc agcgggacga aaucgauaag     2760

uacgcccccg gauucaucaa uagacagcug guugagaccc gccaaauuau caaaacggug     2820

gagcagauua uccaccggcu guggccggac auucagaucg ucacaaucaa ggcuaaccuu     2880

acccaccagg ugagaucuga acucggcuuu ccuaaaguuc gcgagcugaa ugauuaccau     2940

cacgccuucg acgcccugcu cgcugcgcgg auugggcaau aucugcuuga acauuacgcc     3000

ggacugaagc ccuuuuucac auauggcgag uuuaaaagag gggauaccaa gaaguacggc     3060

accacaaacu uccuccgccg gcugacgaaa gacaauaagg auaaaaaggg agagcugcuu     3120

cccgaaguga gcgacuggau cgauuccauu gacucuaucu auaacuacaa aaagauuccu     3180

gucguguggg agaauaccac aaacaugagc gcucuguuug accagaccau cuauccgaga     3240

cccgaucgcg acacaaccaa acggaagcuc auuccuacga agcuggauaa acccacagac     3300

aucuacggcg gguacuccag cgccaagacc gccuauaugg cuguuaugag agugcagacc     3360

aaaaagggaa cagaauaccc ugucuucaau auccccgugu cuauugccuc ccaacucaac     3420

cggaucaccg aucagacaau gcugaaacaa caggugaagc aguuuauuau caaugcggag     3480

cagagcgcuu cuaagaaaaa gcuguaugaa uccggcgacg ucauucuuag ccgcguugcc     3540

cucgggcaga gauucacgga uaaaaacuac ggagaggugu uccugaucuc uaauauggag     3600

gugcagaacc cucaaagccu guggcuuggc cgggccuccc agcagcgccu gaccagagaa     3660

cccgagggga augaaaagcc ggagacaagc cucgcugaca ucuaucagca aauucugaaa     3720

caggucgauc gguacuuucu cgccuucaac aucagacagc aacgccagaa gcugcuuggc     3780

acccuggacc aguuuacauc ucucccugug ggacggguua acgaacagac cagaacggau     3840

caaaaucagc gcuccauuau cgcgcgggug augauugucc ugggcaaggg gagcagagag     3900

gagaaauucc ccgaauuugg acuguccgcu cuuggccgcc uggugcggac cgggggaauc     3960

acacucucug aggacgccca gaucauuuau cagagcccua ccggccuguu cgaaagacgc     4020

guugcccuca aggaucugug a                                               4041


<210>  4170
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4170
auggccaaga uccugggccu cgaccugggg accaacagca uuggaugggc uguggucgau       60

aucgagaaua acaaugaguu cacacuuauu gagaaaggcg uuagaaucuu ucaggaaggg      120

gugaagauug agaaaggaau cgaaggcucc aaggccgcgg agcggacgac cuaccgcucu      180

gcuagacgga ucaaauaucg cagaaagcug cggaagauuc aacuccuggg ggugcuuagc      240

gagaacggau acugccccuu ccuguccaaa gacgaacucg auucuuggcg cuauaagaaa      300

gucuacccua caaauaagag cuuucugaac uggcagaaaa ccaaugagau ccagcuggaa      360

aacccguauu ucuacagagc ccuccuggug gagaagaagc ucaaucugaa cauugaggaa      420

gaccgguuua aaaucggccg cgccuucuau caccucgcuc agagacgggg cuuucugucc      480

aaucgcaugg aggggacaaa ggaaaacgau ggaaccguua uuaaaucuau cacagagauu      540

agcaccgcca agggcgacaa aacgcuuggg caauacuucu acgaaagaua uaagaaagga      600

gagaagaucc gggauaccua cacacaucgc gaggaacacu aucuggagga auuugugaga      660

aucugugagu uccagcagcu cgacaauaag uuugcugaaa aaguccggaa ggccauuuuc      720

uaccagagac cccugaaauc ccaaaagggc cugaucggga aaugcguguu ugagccuaaa      780

aagagccgcu guucuguuuc ccggcccgag uucgaagagu auagaaugcu cugcuuccug      840

aacaauauua aaaucaagac cccuagcgau gaaaaccuuc gcuuucugac agacaacgag      900

aaagaaaagc ucaaacccca guucuuucgg aagucuaagg aucauuucga cuuugaggau      960

cuggugaaac agcuugcgcc uaagaaucag uucaaauuuu acaaguccag agacauucug     1020

ccgggaaacu ggcuuuucaa uuauagcaug aacaccacgg ucucuggcug ucccaucucc     1080

gcccgccuga aagaccuguu uggcgagaau uucauggaua ucaaguuuga cuacauucgg     1140

gaaaaggaug ggaaaaagag cgugaucgac auucacgaua ucuggcaugu ccucuucaca     1200

uacgacuccg aggaaaaacu gaaggaguuu gcuagaaacc gccuuaaucu ggaugacauu     1260

aaaaucaagg aguuccaacg gauccagcuc aaaaaggaau augccgcucu gucucuuaag     1320

gccauuagaa aaauccugcc uuuccuccgc gagggacuga uuuacagcca cgcgguguuu     1380

aucgccaaca uggaaaccgc uauucccucc gagaucugga auaacuauac aaaucggaac     1440

auuaucaaga augaaaucua caacauuauc agcaaucaga ccgaggauaa acaaguugug     1500

gacauuguga acggcaucau uaagucuucc agaaucgaga acagcgucug gucuaaugaa     1560

gccguggagu ucuuuaaaaa ggaucugaac agcaagauca aaaauuauua cgggauuaag     1620

accuauaacg acuucccuga agagaaaaag auccggauug aaacauacgc uuuuaaucuc     1680

uucaaacagc agaugaugaa gaacuuugga cgcggcgagu ucgccgccgu ucagagaauc     1740

gacgagcggc ugaaacuuuu ucugacggau aauuucaaca uugaccccau ccuccugaag     1800

aagaucuacc acccguccgc uauugaagug uaucaaccuc ccgucagaaa gaaugauggg     1860

aagcuguacc uuggaucucc uauggugagc uccauccgca accccauggc gaugcgggcc     1920

cuccaucagc ugagaaaacu gauuaaugag cucauccgca aggaacugau ugacagcaac     1980

accaagguua auaucgagau ggcuagagaa cuuaugaacg ccaaugagcg gaaagccuau     2040

cagaacuggc agaacgaucg cgagaagauc agaaaagaau acgcucugaa gauuaaacaa     2100

caccucgacg uggauaagga gcccucugac gucgauaucc ugaaauauca gcugugggag     2160

gaacagaauc auaagugcau uuacacaggc aaggagauca acauuuccga cuuucuuggc     2220

agcaauccua aauucgauau cgaacacacc auuccgcggu cuaucuccuu ugacaacagc     2280

caguccaaua agacacucug ugagaacuau uucaaucgcu cugugaaaaa gaacagaauc     2340

cccgccgcgc ugccuaauca cggggaaauu aucgagcggc uggaaaacug gaaacucaag     2400

uacgaggagc uggaaaagca aauucaguac gcuguccgcc aggccaaagg agccggcgau     2460

aaggacagca aagacagagc uauccagaag cggcauaaac uuaaguucga guaugauuac     2520

uggaagaaua aauaugaccg cuuugugaug gaagauguuc ccgagggguu caagaacucc     2580

caacuggugg acaccggaau uaucacgaaa uacucuagaa uguaucucaa uacacuguuu     2640

gauaaggucu acaccgugaa aggcaacacc guugccgacu uccggaagcu gugggggauc     2700

caggaugaau augagaagaa agcuagagug aaucacagcc aucacugcgu cgacgccauu     2760

acaaucgcgu guauuaccaa guccaacuac gagaaccuug ccaaauuuua ccaugauugg     2820

gaggaacucc acgugaaggg caucgaccag cugccuccca ugaauaaacc guggaagaca     2880

uucacggaag auauuaaggc uauccaauau gagauccugg ugagccacua caccccugac     2940

aaccucccca aacagucuaa gaaaauucug cgcaagaaag gacggaucca gaagaaugaa     3000

aacggcaauc cuauuuaucu uaaaggggau acaguuagag gaucccugca ucagcaaacc     3060

uuuuacggcg ccaucgagaa gacagagguc aacaagaaag gggaacccga gaaggugauu     3120

aaauaugugg uccgcaagag ccucgacgug cuggacgauu cuuccguuaa aaauaucgug     3180

gacgauauug ucaaggaaau cgugcagaac gcucggauca aggagaaaga cuucauuaag     3240

gagcuggaaa uccagaaaag aaaacuugcc aaggcggagg aaaauaaggu ugugcagauu     3300

caagccgaga ucgauaagcu caacaaacag gucgcugacc uguacaccau gccuaauaag     3360

aacggaaauc ccauuccgau caaaaaggug cgcaucuuuc agacgagcgu uaccaacccu     3420

auucaccuga aacagcaacg ggauaagucu cagaagacaa gcaaacccca uaaggaguuc     3480

uauuacgccg cuaaugacuc caacuacaug auggccaucu augaaggcaa agaugagaag     3540

gggaaaguga agagagacuu uaaacuccug aauaaccuug aagccggcga guucuucaag     3600

uacagcgucc acaaggaucu gaaagaaaag aaccucgaga auauucgcaa cgugaucccc     3660

gacaaagugu cugcuggaaa gauugaaauc ccucugaaag cgaucauuaa gaccggcaca     3720

auggucaucc ugugggaaaa uuccccggac gagguuuggg aucuuaccau uagcgaagug     3780

gacaagcggc uguauaaagu ggucgggaug uccgugaaca gaaucaaguc ugauguuaaa     3840

acguuugagu ucggaaauau ugugcuccgg cacaacaagg aagccagccc cgcuacacug     3900

cucaaaaucc aggacggcga guuuaagucc gaugaaaauu acauugccca acgcaagcug     3960

ucucauaacc aguucaaugc ccuugucgag gggguggacu uuagccuguc cguucucgga     4020

gagaucaaaa gacugaagug a                                               4041


<210>  4171
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4171
augggcgagg acgggaagcc cgugagaguc aaaggaaaga ccgccaucgg cgcucggcug       60

uuccaggaag ggcacguugc cgcggaucgc agaauguuuc ggacaacgcg cagacggcuc      120

aaccgcagaa aauggcggcu gaaacuucug gacgagauuu ucgauccuua caucaccccg      180

guggacagca cauuuuucgc ucgccucaaa caauccaauc ugucucccaa ggauagcaga      240

aaggaauuua aaggauccau gcuuuucccu gaccugaccg auaagcagua ucaugaggac      300

uaccccacaa uuuaucaccu ccggcgcgcc cugaugaccg aagauaaaaa guuugacauc      360

agaauggugu accuggccau ucaucacauc gucaaauauc ggggcaacuu ccucaauucu      420

gcuacagugg auaccuuuaa ggccagcaag guugacuuca aaaaguccuu uaaccagcug      480

aaugagcuuu acucugcuau caacccugag gaugccuucc agguggacau gaauaaaagc      540

gacgaaacgg ggcgccuguu ucucgauucc agcauuagaa aguucgacaa acaaaagcag      600

aucucuaaac ugauucccgu cgugguugag gauaaggaag cgaagaaacu uaacggaaag      660

aucgcccugg agauuaccaa agcuauccuc ggcuauaagu ccaaacugaa uguggucaca      720

cagaccgagc cugaagaccc gaagaagugg gccgugaaau ucgaugacga ggauauugac      780

agcaagcuga acgauauccu ccccgaaaug gacgagaauc agcaaucuau cguggaaauu      840

auccaggauc uguacuccca ggucacacuu aaccagauug uuccuaaugg caugagccug      900

ucugacucca ugaucaaaaa guacaacgac caucacgagc accucaaacu guauaagaaa      960

cuuauugaua auaucagcga caccaaaaag cgguccgcuc ugaaaacggc cuacgauaag     1020

uauguggggg acgauggaaa aacaaucgag caagacgaau uuuacucuaa cgugaagaag     1080

aaccucgaug acagcgaucu guccaaacag auugcggacc ugaucgaggc cgaaaaguuc     1140

augcccaaac agagaaccag ccaaaauggc gucauuccuc aucagcucca ccagcgcgag     1200

cuggaugaaa ucauugagca ucagucuaag uauuaccccu gguuugcuga ggccaacccg     1260

aauaaacacg accuucaccu ggcuaaguau aaaaucgagg agcucguggc cuuccggauc     1320

ccuuacuacg uugggcccau gauuacaccu gaagaucaag agaaguccag ccagaccauc     1380

uuugccugga ugaagagaaa agaacccgga cagauuaccc ccuggaacuu cgacgagaag     1440

guggaccgca uggagucugc uaaucgguuu aucaaaagaa ugacaacgaa ggauaccuau     1500

cugauuggcg aagacguccu uccugaucag agccugcucu acgagaaauu caaggugcug     1560

aacgaacuga auaugguucg cgugaacgac cauccgcuca aggucuccga gaaacaagcg     1620

aucuaucggg aucuguuuga gaaucagaag agaauuucug ugaaaaaccu ucagaauuac     1680

gucaagagca aauccgggcu gcccacagga ccuaagauca gcggccucuc ugaccccgau     1740

cacuucaacu ccagccuggg caccuauaau gacuuuaaga aaauguucgg ggauaaggug     1800

gacgauccug accugcagga ugacuuugaa aagaugaucg aguggucuac aauuuucgaa     1860

gacaagaaaa uccuucgcgc caagcucgau gagguugcuu ggcuguccga cacccaaaag     1920

aacgaguuca aagaaauucg guaccaggga uggggcagac ugagcaagaa acuccugacg     1980

gggaucgugg auggaaaugg ccagcgcauu aucgacgagc uuuggaacac aaacaagaau     2040

uuuaugcaga uccaauccga ugacucuuuc gccaaacgga uucaugaagc caacgcuaau     2100

cagaugaagg ccaccgaugu cgaggacgug cuggcggaug cuuauaccag cccccagaac     2160

aaaaaggcca ucagacaagu ugugaagguc guggacgaua uucagaaagc caugggagga     2220

guggcuccga aguuuaucuc cauugaguuc acacggucug aagaccgcaa uccuagacgg     2280

accaucagca gacagcgcca gcucgaaaac acacugaaag acacggccca agcucugguu     2340

aaguccauca aucccgauau ucuuagcgag cucgacaacg ccucuaaauc caagaagggc     2400

cugaccgauc ggcuguaccu cuacuuuaca cagcugggca aagacaugua uaccgggaag     2460

ccuaucaaua uugaugagau cagcaacuac gacauugauc acaucuuccc ccaggcguuu     2520

aucaaagacg auucucuuaa uaacagaguc cuggugucca aggccggaaa uaacggcaaa     2580

agcgcuaaug ugccucucaa gcuguucggg gccaagaugg gaggcuauug gaaacagcug     2640

gcugacgccg ggcuuauugg ccaacgcaag cucaaaaacc ugcagacaaa ccccgauacc     2700

aucucuaagu uugcgaugca uggauucauu agacggcagc uggucgaaac gagccaggug     2760

aucaaacucg uugccaauau ucugggcgac aaguaccgca acgaugacac caagaucauu     2820

gagaucacag cuagaaugaa ucaccaaaug cgggacgaau uuggguucau caaaaaccgc     2880

gagauuaaug auuaucacca ugccuuugac gcuuaccuua ccgccuuccu gggacacuau     2940

cucuaccaua gauacaucaa gcugcggccg uauuucgugu acggcgauuu uaaaaaguuc     3000

cgcgaagaca aagucacaau gagaaacuuu aauuuccugc acgaucuuac cgacgauaag     3060

caggagcgga uugccgacac ggagacaggg gaagugaucu gggaucgcaa gagauccauu     3120

cagcaacucc gggacguuua ucacuacaaa uuuaugcuga ucagccagga gguguucucu     3180

cugaacaagg aaauguuuaa acagaaacuc uucccugcua cccuggcgaa gaaguccaaa     3240

cuuauccaga uuaagaagga uaaacccguc gacaucuaug gaggcuacag caauaacaca     3300

gaugccuauc ugaccguggu uaagcucaaa ggguccaagg aggacaaaua caagauugug     3360

ggcgucccca ugagagcucu ggacaaucug aagcaugccg agaaagaauc uccucaaagc     3420

uacaccgccg agcuucgcaa ggcucuggaa ccgcaguuua caaaaaucaa gaaaaaccgg     3480

aagacgggag agguguccaa gguggucacc aauuucgaua uuguucucgg cagacuguuu     3540

uaucgccaga uggugaucga cgggucugaa aagagccuca ucgccuccuc uguguaccag     3600

uauaacgcga agcaacuggu ccuuagcgau caggcuauuc ggacaaucuc cgaccaggag     3660

ggaauugaug aguucgaacu gagcucucag uacgacaaag uguaugauga gaucguucaa     3720

aagguggaaa aacuccugcc cauuuuccag cacagacauu uucaggagcg ccugaauaac     3780

ggcaaguccc uuuucgucaa ccugccugug cacgacguuu acgaagggaa uaagcucgaa     3840

caggugggga aaaagcauau cauuaacagc auccugggcg ggauccacgc caaugccgau     3900

cggcccgaca uugaucucuu uggcaucaaa aaccugggac aauucucuuc caccagcggc     3960

auuaaucuuu cuuccgacgc uaucauuugc uaucagagcc cuacagggcu guuugagaga     4020

cgcgucgugc ucaaggaccu guga                                            4044


<210>  4172
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4172
augaccaaga gagaggaacc cuacaacgug ggccuggaca ucgggagcuc cucugucgga       60

ugggccguug uggauaauaa cuaucaccuc cugaauauua aaaagaacaa ucuuuggggc      120

gcucggcugu ucaaagaggc cgaaacagcu caggugacgc gcgggcauag aagcaugcgg      180

cgcagauacc ggcgcagacg gaaccgccuc aauuggcugg acgagcuuuu ugcggaugaa      240

cuggccaaga ucgacccuuc cuuccuccug agaaugaaaa acucuugggu cagcaagaag      300

gaguccaccc ggaaacgcga uccguauaau cuguuuauug acgagaagua caacgaugug      360

gacuauuuca aucaauaccc cacaaucuuu caccucagaa aagaacugau uaccgaggau      420

aagaaaguug acauccggcu uaucuaucug gccauucaua acaucauuaa guaccgcgga      480

aauuucacac ucgaaaacca gaauuuugau aucucucagc ugagcuccaa cuucagccag      540

caaauuucug acuuuuucgc ucuguuuucc gauuucggcg ugaucaugcc ugaggacuuu      600

gaccccgaua agauuagcga cauccugcuc aacccuaauc ugucucccuc cgggaaaguc      660

agcgaggcca ucgcuaccau uucuccuaag acaaacguga aagccaagau caaaauuauc      720

cugcuccugc uuguuggaaa uaacggcgau cugaagaaac ucuucgaccu ggaaaccacg      780

gagaagauug cggugaaacu uuccagcaga cacaucgauu ccgaacugcc gaucauucuc      840

ucugagcuga augaacagca ggagaacauc auuaccaucg ccaauagcau uuucggcucc      900

aucauccuga aagacuuucu cggggaugag acaagcauuu cugcugccaa ggucaucucc      960

uucgaagacc auaaacagga ucugcaaaaa cuuaaaacca uguggcggga gacaagcaac     1020

aaggaagcug ugaaggccgg aaagaaggcg uacgaggacu auauuggcca cgaagauucu     1080

gagaccuuuu acaaaaagau caaaaaguuc cuggagaagg cccagcccgu ggaccucgcu     1140

aauaaagccc uggcugaaau ugagcuugaa aacuaucugc cuaagcagag aaaucgcaac     1200

aauacgguca uccccuacca gcucaacgag aaugaacuga uugccauccu ggaccaccaa     1260

gagaaauauu acccuuuucu caaggagaac cgggauaaaa uccugagccu ucugacauuc     1320

cgcauucccu auuacguugg gccgcuccag gacuccaaca auaacagauu uucuuggaug     1380

acccggaagg ccagcggagc uaucagaccu uggaauuucu ccgaaaaggu gaacguggag     1440

cagagcucua augauuuuau uaaacgcaug cgguccacag acaccuaccu gaucggcgaa     1500

cccguccugc cuaagaaaag ccuuauuuau caaugcuacg aggugcucuc ugagcugaac     1560

aauaccagag uuaaggaugg guccagcaac cccaaacgcc uggacgugac aaucaagcag     1620

cggaucuaua augaaauuuu caaaaaccag aaguccgucu cugugaaggu ucuccagaau     1680

uggcugauca aagagagcua cuuuaagucc cccgaaauuu cuggacuugc ggauaaaaag     1740

aaauaucuga gcucccucag cacguacauc gacuucaaga agauuuuugg ccaaagauuc     1800

guggaugacc cugucaacuc uccgcagcug gaggaacugg ccgaguggcu uacccucuuc     1860

gaggauaaaa agauccugcu gaucaaacuc caaaauucca aguauagcua cgaccaggcu     1920

acaauuaaca aacugucuac caugcgcuac cagggcacag ggaaacuuuc caagaagcug     1980

cucguggauc ugaagaccac gaaaaagagc aucggaaaau cuggcgccga aagccugucc     2040

auucuugacc ucauguggag cacaaaggac aauuuuaugc agaucauuca ugaugccgac     2100

uauaccuucg agcagcagau caaggaauuu aacuacgaua ccgaggacga acugacaccc     2160

cuggagaaag ucgcuaaccu ccacgggucu ccugcccuga agcggggacu uaaucaaucc     2220

aucaaagugg uugcggauau ugugaaguuc augggccaug accccgagaa aaucuuuauu     2280

gaguucacca gaagcgauga cuuuuccaag cugacaaucu cucgguaucg cagaauuaaa     2340

aagcaguacc ucgaaaucgc uaaggccauu aaaaagaucc cugccgaguu caaagauauc     2400

aaggaauauc agacgcagcu ggaagagaac aaagggaagc uggcuagcga gcggcuuaug     2460

cucuacuuuc ugcaaugugg acacucccug uauucuaaua agcccauuga ccucaacaug     2520

aucaauagcu ccaaauacca cgucgaucau auucugccgc agucuuacau caaggacgau     2580

agccuugaaa acaaagcccu ggugcucgcu uccgagaaug aaaacaagau ugacaauaug     2640

cugaucagcc acgacaucau ugccaccaac cugccuagau ggcaggcgcu uaaagaucaa     2700

aaucugaugg gcucuaagaa guucgccgac cucacacgca ccacaguuac cgagaaccag     2760

aaaaagggcu uuauccagcg gcagcuggug caaacguccc agauugucaa aaauaucacc     2820

cucauucuga acgaucuuua uaagaauaca agcugcaucg agaccagagc uacacugucu     2880

uccgaguucc gcaaagccuu uagcaacuuc gacgaaacca cguaccauua ucaguucccc     2940

gaauuuguga agaacagaga ugugaaugac uuccaccaug cucaggaugc cuuucucgcg     3000

uguguuaucg gggaguacca acugaagaaa uauccuaagg acaaccugcg gcuugucuac     3060

gaucaguauu cuaaauuccu ggacucccuc aagaaagaua cacgcaagaa gaauggaaga     3120

augccccggu acacccagaa cggcuuuauu aucgggagca uguucaaugg aaaaacauac     3180

guggacgaca acggcgaaau uaucugggau cagaagauua aagaguccau ccgcaagacc     3240

uuuaauuauc accaauucaa cguggucaga cagaccauug agcagcacgg gaaacuguuu     3300

aaugacacaa uccaaccuca uagcgaucgg uacaagcuca ucccccugaa aacgaaccgc     3360

gacccggcca uuuauggcgg auacaauaac gauaauaacg cuuauucugu gguucuugac     3420

guggauggca agaagaaaau caaugggauu ccuaucagaa uugccaacca gcuuaagagc     3480

gacgaacucg aucuguccuc uuggcuggag aauaacauca aacacaagaa acccaugacc     3540

auccuuauug acaagguccc caaguaccag cggaucauua acgaagagac aggagaucug     3600

cucaucacca gcgcuaauga agugauuaac aauguucagc uguuccuccc uuccauguau     3660

acagcccuga ucucucuucu ggacagcacc aaaacggaga uguacuccaa gcugcucucu     3720

aacuacgagg ccaauauccu gauugauauc uaugacuacc uucugacaaa acucaagaac     3780

aauuauccgc uguaccgcaa agaaugggcu aagcucgcgg agcauagaga cgauuuuauu     3840

gaaagcgacc uggugaccca agccuccacc cuucagcagc ugaucaaguu caugcacgcu     3900

gaucccagca acgucaaucu gaaauucggc aacuuuaagg ggaaucgguu cggaagaaag     3960

aacggcaaua uuaagcucuc uaaaacagac uuuaucuaug aguccccuac cgggcuguuc     4020

aagagcauua agcacaucga uuga                                            4044


<210>  4173
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4173
augaaguccg agaaaaagua cuauaucggc cuggacgugg ggaccaacuc cgucggaugg       60

gccguuacag augaguucua caauauucuc agagcuaaag gcaaggaccu guggggagug      120

cggcuuuuug agaaagccaa gacggcggcu gauacccgca cauucagauc ugggcggcgc      180

agaaacgacc ggaagggcau gcgccugcag auccucagag aaauuuuuga ggaugaaauc      240

aaaaaggugg acaaagauuu cuaugaccgg cuggaugaga gcaaguuuug ggccgaggac      300

aaaaaggucu ccgggaagua cucucuuuuc aaugauaaaa acuuuagcga caagcaauau      360

uucgaaaaau uucccaccau uuuccaucug cgcaaguacc ucauggagga acauggaaaa      420

guggauauca gauauuacuu ucuggccauc aaucagauga ugaagcggcg cggccacuuc      480

cugauugacg gccagaucuc ccauguuaca gacgauaaac cucucaagga gcagcugauu      540

cuucugauca acgaccuccu gaagauugag cuugaagagg aacugaugga uucuaucuuu      600

gagauucucg cugacgugaa ugagaaaaga accgauaaga aaaacaaucu gaaggaacug      660

aucaaagggc aagacuucaa caagcaggag ggaaauaucc ucaacagcau uuucgaaucc      720

aucgucacag gcaaggccaa aauuaagaau aucauuagcg augaggacau ccuggagaaa      780

aucaaggaag auaacaaaga agacuuugug cuuaccgggg auucuuacga ggagaaucug      840

caguauuucg aagagguucu ccaggaaaac auuacgcugu uuaacacccu uaaguccaca      900

uacgacuucg ugauccugca aagcauucuc aagggaaaau cuacccuguc cgacgcucag      960

gucgagcggu augaugaaca caagaaagac cuggagaucc ucaagaaagu gauuaagaag     1020

uacgaugagg acggcaaacu guuuaaacag guguucaaag aagauaaugg gaacggauau     1080

gucagcuaca ucggcuauua ccuuaauaag aacaaaaaga ucacagccaa aaagaagauu     1140

ucuaauaucg aguuuaccaa auacguuaag ggcauucugg aaaaacagug cgacugugag     1200

gaugaggcgg ugaaguaucu ccuggggaaa aucgaacaag agaacuuccu ucugaagcag     1260

auuuccagca ucaauuccgu gauuccgcau cagauccacc ucuuugaacu ggacaagauc     1320

cuggagaacc ucgccaaaaa uuaccccucu uucaacaaua agaaggaaga auuuacgaag     1380

auugagaaaa uccgcaagac auucaccuuu cggauuccuu auuacgucgg accccugaac     1440

gauuaucaca agaauaacgg cgggaaugcu uggaucuuca gaaacaaagg agaaaagauu     1500

agaccuugga acuucgagaa aaucguggac cuucacaaga gcgaagagga auuuaucaaa     1560

cgcaugcuga aucaaugcac auaccucccc gaggagaccg uucugccuaa gagcagcauu     1620

cuguauucug aauacauggu gcuuaacgag cucaauaacc ugcggaucaa gggcaaaccg     1680

cuggauaccg acgucaagcu caaacugauu gaggaacuuu ucaagaaaaa gacaaaagug     1740

acgcugaagu ccaucagaga uuacaugauu cgcaauaacu uugccgacaa ggaagacuuc     1800

gauaauagcg agaaaaaccu cgagaucguu ucuaauauga aaagcuauau cgacuuuaac     1860

aauauucugg gggauaaguu cgaacuggag augguggaag accuuaucga gaagauuacc     1920

auccacacag gaaacaaaac ccuccugaag aaguacauug aggaaacaua ucccgaucug     1980

uccucuagcc agauccagaa aaucauuaau cucaaguaca aagacugggg ccggcugucc     2040

agaaaacuuc uggauggcau caaggggcgc aacaagaccg acgaugucaa uacggugauu     2100

aacuuuaugc ggaacagcuc ugacaaucuc augcagauca uugagggaaa gaacuauucc     2160

uucaaugaau acaucgauaa ccugcgcaaa aaguauauuc cucaagagau cagcuacgaa     2220

gucguggaga aucuguacgu uucucccucc gugaaaaaga ugaucuggca ggucauuaga     2280

gugacagagg aaaucaccaa aguuaugggc uaugacccug auaagauuuu uaucgagaug     2340

gcuaagagcg aagaggagaa aaagaccaca auuucccgga aaaacaaacu ucucgaccug     2400

uacaaggcca ucaagaagga cgaaagagau ucucaguaug agaaacugcu caccgggcug     2460

aauaaacuug acgaaagcga gcugcgcucc aagaagcucu accuguauua cacacagaug     2520

ggacgggaua uguauacggg cgagaaaauc gaccuggaua aacuuuucga cucuacccau     2580

uacgauaagg accacauuau cccccaaagc augaagaagg augacuccau uaucaacaau     2640

cucgugcugg ucaacaaaaa ugcgaaccag acaaccaagg ggaauauuua cccggugccu     2700

agcucuauca gaaacaaucc cgauaucuau aacuacugga aauaucugau ggaaaaggaa     2760

uuuauuucca aagagaagua caaccggcuc auccgcaaua caccucugac caacgaggaa     2820

cuuggcgugu ucauuaauag acagcugguu gagacgcggc agagcaccaa ggccaucaaa     2880

gaacucuuug agaaguucua ucaaaaaucu aagauuaucc ccgucaaagc cucccuggcu     2940

agcgaccuga gaaaggacau gaacacacuu aagucucgcg aggugaauga ucugcaccau     3000

gcucacgacg ccuuccucaa cauuguggcc ggagaugucu ggaaucggga auuuaccagc     3060

aaccccauca auuacgugaa cgagaauaga gaaggcgaca aaguuaagua uucccugagc     3120

aaauacuuca cacgcccuag aaagucuaaa gggaagguga ucuggacccc ggagaaggga     3180

cggaaacuca uugucgauac gcugaacaag cccuccgugc uuaucagcaa ugaaucccau     3240

guuaaaaagg gcgagcuguu uaacgcuaca auuucuggga aaaaggacua caaaaaggga     3300

aagaucuauc ucccucugaa aaaggaugac cgccugaaag augugagcaa guacggcggg     3360

uauaaagcga uuaauggcgc cuucuuuuuc cuugucgagc acaccaaguc caagaaaaga     3420

auccggucua ucgaacuguu uccccuccau cugcucagca aguucuacga ggacaaaaac     3480

acagugcugg auuaugcuau uaauguucuu cagcugcagg acccuaagau cauuaucgau     3540

aaaauuaacu accgcaccga aaucaucauu gacaacuuuu ccuaucucau cucuaccaag     3600

agcaaugacg gauccauuac agugaagccc aacgagcaga uguacuggag agucgaugaa     3660

aucagcaauc ugaaaaagau ugagaacaaa uacaagaaag acgccauccu gacggaggaa     3720

gaucggaaga uuauggaguc uuauaucgac aagaucuacc aacaguucaa agccggcaag     3780

uauaaaaauc gcagaaccac agauaccauu aucgaaaagu acgagauuau cgaccuugau     3840

acacuggaca acaaacagcu cuaucaacug cucguggcuu uuauuucccu gagcuacaag     3900

accucuaaua acgccgugga uuucacgguc aucgggcuug gaacagaaug uggcaaaccg     3960

cggaucacca aucugccuga caacaccuau cugguuuaca aguccauuac agggaucuac     4020

gagaagcgca uuagaaucaa auga                                            4044


<210>  4174
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4174
augaagaaac ccuacaccau cgcccuggac auuggcacaa acagcguggg gugggucguu       60

gugacggaug acuauagagu gccuaccaag aaaaugaagg uccucggaaa uacagagcgg      120

aaaaccauca agaagaaccu gauuggcgcc cuucuguucg auuccgggga cacagccgaa      180

ggaacccgcc ucaaaagaac agcgcggcgc agauacaccc ggcgcaagaa uagacugcgg      240

uaucuuaaag agaucuuuac ggaagagaug gcuaaggugg augacggcuu cuuucagcgc      300

cuggaagauu cuuucuacgu ucucgaggac aaagagggga acaagcaccc gauuuuugcc      360

aaucuggccg augaaguggc uuaucauaag aaauacccca ccaucuauca ccugagaaag      420

gagcucgucg acaacccuca aaaagccgau cugcggcuua ucuaccuggc uguggcccau      480

auuaucaagu uccgcggaca cuuucucauu gaaggcacac ugagcuccaa aaauaacaau      540

cuucagaagu cuuucgacca ucugguugau accuacaacc uccuguuuga ggagcagaga      600

cugcucacag aaggcaucaa ugcgaaagag cugcuuagcg ccgcucuguc caagucuaag      660

cggcucgaaa accugauuag ccuuaucccc gggcagaaaa agaccggaau uuucggcaau      720

aucaucgccc ugucccucgg gcugacgccu aacuuuaaag cuaauuucgg acugagcaag      780

gacgugaaac uccaacuggc caaggacaca uaugcggaug accuugauuc ucugcucgcc      840

cagauuggcg accaguacgc ugaucuguuu cuugccgcua agaaccuguc cgacgccauc      900

cuccugagcg auauucugac cgagucugac gaaaucacac gcgcuccccu cuccgcuagc      960

auggucaaaa gauaucggga gcaccacaag gaucugguga cccuuaaaac ccugauuaag     1020

gaccagcucc cugagaaaua ccaagaaauc uuccuggaua agacaaagaa cggguaugcg     1080

ggauacaucg agggccaggu gucucaggaa gaguucuaua aauaccugaa gccgauucuu     1140

gccagacucg acggcuccga accccugcug cucaaaaucg accgcgagga uuuucugcgg     1200

aagcagagaa cguucgacaa ugggagcauu ccucaucaaa uccaccuuga ggaacugcau     1260

gcuauucucc gccggcagga ggucuuuuac cccuuccuga aagauaacag aaagaagauc     1320

gaaucccugc uuaccuuucg caucccuuau uacguuggac cccucgcccg gggccacucu     1380

agauucgccu gggugaaacg caaguuugac ggggcuauuc ggccguggaa uuucgaggaa     1440

aucguggaug aggaggccag cgcgcagauu uuuaucgaaa agaugacaaa gaacgaccug     1500

uaucugccua augagaaagu ccuccccaag cacucccugc uuuacgaaac cuucacagug     1560

uauaacgagc ugaccaaagu uaaguacgcu acggagggaa ugacaagacc ucaauuucuc     1620

agcgccgauc agaagcaggc cauuguggac cugcuguuca aaaccaaucg caaggucacc     1680

gugaaacagc uuaaggaaaa cuauuuuaaa aagaucgagu gcugggauuc uguugaaauu     1740

acaggcgugg aggacuccuu caaugcuagc cucgggaccu accaugaucu gcugaagauc     1800

auccaagaca aagauuuccu cgacaacccc gacaaucaga agauuaucga agauauuauc     1860

cugacacuua cgcuguuuga ggacaaaaag augauuucua aacggcucga ucaguacgcc     1920

caccuguucg acaaggucgu gcugaacaaa cuugagagac aucacuauac cggauggggc     1980

cggcucagcg gcaaacugau caaugggauc cgcgauaagc aguccggaaa aacaauucug     2040

gacuuucuca aggcugaugg cuucgccaac agaaauuuua ugcaacugau ccaugacucu     2100

gaacuuagcu ucauugauga gaucgcgaaa gcccagguca uugggaagac cgaauacucc     2160

aaggaccugg ugggaaaccu cgcuggcagc cccgccauca aaaaggggau cucucagaca     2220

auuaaaaucg uugaugagcu ggugaagauu augggcuauc ugccucagca aaucgucauu     2280

gaaauggcuc gggagaacca gaccacggcc cagggaauca aaaaugcgag acagcgcaug     2340

cggaaacuug aggaaaccgc caagaagcug ggcuccaaca uccucaagga gcacccggug     2400

gacaauagcc aacugcagaa cgauaaaaga uaccucuauu accugcagaa ugggaaggac     2460

auguauacag gagacgaucu ugacauugau uaccugucuu ccuacgacau cgaucacauu     2520

aucccccaga gcuuuauuaa aaacaauucc aucgacaaca agguucucac cucucaaggc     2580

gcuaaucgcg ggaaacugga uaacgugccu agcgaagcca uugucagaaa gaugaaggga     2640

uauuggcagu cccugcuucg ggcuggcgcc aucucuaaac agaaguucga caaucugaca     2700

aaagccgagc gcgggggccu cacccaagug gauaaggcug gauuuaucca gagacagcug     2760

guggaaacgc ggcagauuac aaaacauguu gcgcaaaucc ucgacagccg cuucaacacc     2820

gaguuugaug accacaauaa gcggauuaga aagguccaua ucauuacacu gaaauccaaa     2880

cuugugagcg acuuccgcaa ggaguuuggc cuguacaaga ucagagauau caaccacuau     2940

caccaugccc acgacgcuua ccucaaugcc guggucgcca aagcuauucu ggggaaguau     3000

ccccagcugg ccccugaauu uguguacgga gauuauccca aguacaacuc uuucaaagag     3060

cggcagaagg cgacccagaa aaugcuuuuc uacuccaaca uccugaaguu cuuuaaagac     3120

caagaaagcc uccauguuaa uucugauggc gaggagauuu ggaacgcuaa uaagcaucug     3180

ccgaucauua agaacgugcu cuccaucccu caggucaaua ucgugaaaaa gaccgaaguu     3240

cagacagggg gauucuauaa agagagcauu cugucuaagg gcaacagcga caaacuuauc     3300

ccccgcaaga auaacuggga uacgagaaaa uacgggggcu uugacucccc uaccguggcc     3360

uauagcgucc uggugauugc caagauggaa aagggaaaag cuaagguucu gaaacccgug     3420

aaggagaugg ucggcaucac aauuauggaa cggaccgccu ucgaggagaa uccuguggug     3480

uuucucgaag cgagagggua ccgcgagauc caagaacacc ugauuaucaa acuucccaag     3540

uauucucugu ucgagcucga aaacggacgg agacgccugc ucgcuuccgc cagcgagcug     3600

cagaagggca augagcuuuu ucugccgguc gauuacauga cauuccugua ucucgccgcu     3660

cacuaccacg aacugaccgg guccucugag gacguucuuc ggaaaaagua cuuuguggau     3720

agacaucugc acuauuucga cgauaucauu cagaugauca acgacuucgc cgaacgccau     3780

auucucgcua gcuccaaucu ggagaaaauc aaccacacgu accauaauaa cucugaccuc     3840

ccugugaacg aacgggccga gaauauuauc aacgucuuua cauucguggc gcugggagcc     3900

cccgcugccu uuaaguucuu ugaugcuacc aucgacagaa aacgcuauac cagcacaaag     3960

gagguucuua augccacccu gauucaccag uccgugacag gccuguacga aacgcggauc     4020

gaucucucuc aacuggggga gaacuga                                         4047


<210>  4175
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4175
augaaaagcg agaaaaagua cuauaucggc cuggacgugg ggaccaacuc cgucggaugg       60

gccguuacag augaguucua caauauucuc agagcuaaag gcaaggaccu guggggagug      120

cggcuuuuug aaaaagccaa gacggcggcu gauacccgca cauucagauc ugggcggcgc      180

agaaacgacc ggaagggcgu gcgccugcag auccucagag aaauuuuuga ggaugaaauc      240

aaaaaggucg acaaagauuu cuaugaccgg cuggaugaga gcaaguuuug ggccgaggac      300

aaaaaggugu ccgggaagua cucucuuuuc aaugauaaaa acuuuagcga caagcaauau      360

uucgaaaaau uucccaccau uuuccaucug cgcaaguacc ucauggagga acacggaaaa      420

guugauauca gauauuacuu ucuggccauc aaucagauga ugaagcggcg cggccacuuc      480

cugauugacg gccagaucuc ccaugugaca gacgauaaac cucucaagca gcaacugauu      540

cuucugauca acgaccuccu gaagauugag cuugaggaag aguuuaugga uucuaucuuc      600

gaaauucugg cugacgucaa ugagaaaaag accgaaaaga agaacauccu caaagagcug      660

aucaaugccc aggauuucaa caagcaggag gggaagauuc uguacagcau cuuugaaucc      720

auugugacag gaaaagcuaa gaucaaaaau auuaucagcg acgaggauau ccucgaaaag      780

auuaaagagg acaacaagga agauuucguu cugaccggcg acucuuauga ggagaaucuu      840

caguacuuug aagaggugcu gcaagaaaac aucacccucu ucaauacgcu gaaguccgcc      900

uaugauuuug ucauucuuca gagcauccug aaagggaagu cuacacucuc cgacgcccag      960

guggagagau acgacgaaca caaaaaggau cuggagauuc ugaaaaaggu gaucaagaaa     1020

uaugacgagg auggaaaacu cuucaaacag gucuuuaagg aagacaacgg caacggguac     1080

guuagcuaua ucggauacua ccugaauaaa aacaagaaaa uuaccgcgaa gaagaaaauc     1140

ucuaauauug aguucacaaa guaugugaaa ggcauccuug aaaagcaaug cgauugugag     1200

gacgaggaug ugaaauaccu gcucggcaag auugaacagg agaacuuucu gcuuaagcag     1260

aucuccagca uuaauuccgu caucccgcau caaauccacc uguucgaacu cgacaaaauu     1320

cuggagaacc uggcuaagua uuaccccucu uuuaauaaca aaaaggaaga guucaccaaa     1380

aucgagaaga uucggaagac guuuacauuc cgcaucccuu auuacguggg gccccucaau     1440

gauuaucaca aaaacaaugg aggcaacgcc uggauuuuca gaaauaaggg ggaaaagauc     1500

cggccuugga acuuugagaa gaucguugac cugcauaaaa gcgaagagga guucauuaag     1560

agaaugcuua aucagugcac cuaccugccc gaagagacag ugcucccuaa guccagcauc     1620

cuguacucug aauauauggu ccugaacgag cuuaacaauc uccgcauuaa cggaaaaccg     1680

aucgauaccg acgugaagcu gaaacugauu gaggaacucu uuaagaaaaa gaccaaaguu     1740

acacugaagu ccauccggga uuacaugauc agaaauaacu ucgcugacaa ggaagacuuu     1800

gauaauagcg agaaaaaccu ugagauugug ucuaauauga aaagcuauau cgacuucaac     1860

aauauucugg gcgauaaguu ugaacucgag auggucgaag accugaucga gaagauuacg     1920

auccacaccg ggaacaaaaa gcugcuuaag aaauacaucg aggaaacaua ucccgaucuc     1980

uccucuagcc agauucagaa gaucauuaau cugaaauaca aggacugggg acgccugucc     2040

cggaaacucc uggauggcau caccggcaca aacauugaga ccggggaaau cgacacggug     2100

auuaauuucc uuagaaacag cucugauaau cugaugcaaa ucaucggauc ccagaacuau     2160

agcuuuaaca augacauuga uaagcuccgc aagaaauaca ucccucagga gauuucuuac     2220

gaagucgugg agaaccugua uguuuccccc agcgugaaga aaaugaucug gcaggucauu     2280

cggaucacag aggaaaucac caaggugaug ggcuacgacc cugauaaaau uuucaucgag     2340

auggccaagu ccgaagaggg gaagaaaacc acaauuucua gaaagaauaa acugcuugcg     2400

cucuauaagg ccaucaaaaa ggacgaacgc gacagccaau acgagaagcu gcugaccgga     2460

cucaacaaac uggaugacuc cgaucuucgg ucuagaaagc uguaucucua cuauacacag     2520

augggccgcg acauguacac gggggagaaa auugaucugg acaagcuguu ugauagcacc     2580

cauuacgaca aagaucacau caucccccag uccaugaaga aagacgauag cauuaucaau     2640

aaccuuguuc ucgugaauaa gaacgcuaau cagacaacca agggcaacau uuauccgguc     2700

ccuucuucca uccggaauaa ccccaaaauu uacaauuauu ggaaguaccu gauggaaaaa     2760

gaguucauca gcaaggaaaa auauaacaga cugauucgga auacaccucu caccaacgag     2820

gaacugggag gcuuuaucaa ccgccaacuu guggagacga gacagucuac caaggccauc     2880

aaggagcugu ucgaaaaauu cuacaagaaa uccaagauua uccccgugaa agcuauucuc     2940

gccagcgacc ugcggaagga caugaauaca cugaagucua gagagguuaa cgaucuucac     3000

caugcccacg acgcuuuucu gaauaucguc gcgggggaug uguggaaccg cgaguucacc     3060

agcaauccca uuaacuaugu gaaagaaaau cgggagggag acaaggucaa auacucccuc     3120

agcaaguacu uuacaagacc ucgcaaaucu aagggcaagg ugaucuggac cccggaaaaa     3180

gggagaaagc ugaucguuga uacgcucaac aaacccuccg ugcugauuag caaugagucc     3240

caugucaaga aaggagaacu uuucaacgcc acaaucgcug gcaagaaaga cuauaagaag     3300

gggaaaauuu accugccucu caagaaagau gaccggcugc aggauguguc uaaguauggc     3360

ggauacaaag ccaucaaugg cgccuuuuuc uuucugguug agcacaccaa gagcaagaaa     3420

cgcauuagau ccaucgaacu uuucccccug caucuccugu cuaaguuuua ugaggacaaa     3480

aacacagugc ucgauuacgc uaucaauguc cugcagcuuc aagacccuaa gauuaucauu     3540

gauaaaauca acuaucggac cgagauuauc auugacaauu ucagcuaccu gaucuccacc     3600

aagucuaacg acgggagcau cacagugaag cccaaugaac agauguacug gcgcguugau     3660

gagauuucca accucaaaaa gaucgaaaac aaauauaaga aagacgccau ucugacggag     3720

gaagauagaa agaucaugga gagcuacauu gacaagaucu aucagcaauu uaaagcggga     3780

aaguacaaaa aucggcgcac cacagauacc aucauugaga aguaugaaau cauugaccug     3840

gauacacuug acaacaaaca gcuguaccag cuccuggugg cuuucaucuc ucucuccuau     3900

aagaccagca auaacgccgu cgauuucacg gugauuggcc uggggacaga guguggaaaa     3960

ccgagaauca ccaaucuucc ugacaacacc uaccuggugu acaagucuau cacaggcauu     4020

uaugaaaagc ggauccgcau uaaauga                                         4047


<210>  4176
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4176
augaaaagcg agaaaaagua cuauaucggc cuggacgugg ggaccaacuc cgucggaugg       60

gccguuacag augaguucua caauauucuc agagcuaaag gcaaggaccu guggggagug      120

cggcuuuuug agaaagccga uacggcggcu aacacccgca ucuucagauc ugggcggcgc      180

agaaaugacc ggaagggcau gcgccugcag auucucagag aaaucuuuga ggaugaaauu      240

aagaaagugg acaaggauuu cuaugaccgg cuggaugaga gcaaauuuug ggccgaggac      300

aagaaagucu ccgggaagua cucucuuuuc aacgauaaga auuuuagcga caaacaauau      360

uucgaaaagu uucccacaau cuuccaucug cgcaaauacc ucauggagga acacggaaag      420

guggauauca gauauuacuu ucuggccauu aaccagauga ugaaacggcg cggccacuuc      480

cugaucgacg gccagauuuc ccauguuacc gacgauaagc cucucaaaga gcagcugauc      540

cuucugauua augaccuccu gaagaucgag cuugaagagg aacugaugga uucuauuuuu      600

gagauccucg cugacgugaa cgaaaagaga acagauaaaa agaauaaccu gaaagagcug      660

aucaaggggc aagacuucaa uaaacaggag ggaaacauuc ucaauagcau cuucgaaucc      720

auugucaccg gcaaggccaa gaucaaaaac auuaucagcg augaggacau ccuggaaaag      780

auuaaagagg auaauaagga agacuuugug cuuacagggg auucuuacga ggagaaccug      840

caguauuucg aagagguucu ccaggaaaac aucacccugu uuaauacgcu uaaauccacc      900

uacgacuucc ugauucucca aagcauccug aagggaaagu cuacacuguc cgacgcucag      960

guggagcggu augaugaaca caaaaaggac cucgagauuc ugaaaaaggu caucaaaaag     1020

uacgaugagg acggcaagcu guuuaaacag guguucaagg aagauaacgg gaauggauau     1080

gugagcuaca ucggcuauua ccugaacaaa aauaagaaaa uuaccgccaa gaaaaagauc     1140

ucuaacauug aguuuacaaa guacgucaaa ggcauccucg aaaagcagug cgacugugag     1200

gaugaggacg uuaaauaucu gcuugggaag auugaacaag agaauuuccu gcucaaacag     1260

aucuccagca uuaacuccgu gaucccgcau cagauccacc uguuugaacu ggauaagauu     1320

cucgagaauc uggcgaagaa cuaccccucu uucaauaaca aaaaggaaga guuuaccaaa     1380

aucgagaaga uucgcaaaac guucacauuu agaaucccuu auuacguggg accccuuaau     1440

gacuaucaca agaacaaugg cgggaacgcc uggauuuucc ggaacaaggg agaaaagauc     1500

agaccuugga auuucgagaa gaucgucgau cugcauaaaa gcgaagagga auuuauuaag     1560

cgcaugcuca accaaugcac cuaccugccc gaggagacag ugcugccuaa auccagcauc     1620

cuuuauucug aauacauggu ucucaaugag cugaacaauc ugcggauuaa cggcaagccg     1680

cucgacaccg augugaagcu gaaacuuauc gaagaacugu ucaagaaaaa gaccaaaguc     1740

acacucaagu ccauuagaga cuacauggug cgcaauaacu uugcugacaa agaagauuuc     1800

gacaauagcg agaagaaccu ggagaucgcc ucuaauauga aaagcuauau cgauuuuaac     1860

aauauucugg aagacaaguu cgauguugag augguggaag accuuaucga gaagauuacg     1920

auccacaccg ggaacaaaaa gcuccugaaa aaguacauug aggaaacaua ucccgaucug     1980

uccucuagcc agauccagaa gaucauuaau cucaaauaca aggacugggg acggcugucc     2040

agaaaacuuc uggauggcau caagggcacc aaaaaggaga cagaaaagac cgacacgguc     2100

auuaacuuuc uccgcaacag cucugauaau cugaugcaga ucauuggguc ccaaaacuau     2160

agcuucaaug aguacaucga caaacugcgg aagaaauaua uuccucagga aaucucuuac     2220

gagguggucg agaaccuuua cguguccccc agcguuaaga aaaugaucug gcaagugauu     2280

agagucacag aagagaucac caaggugaug ggauaugauc cugacaagau uuuuaucgaa     2340

auggcuaaau ccgaggaaga gaagaaaacc acaauuucuc gcaagaauaa acuccuggac     2400

cuguacaagg ccaucaagaa agaugagcgg gacagccagu augaaaagcu ccugaccggc     2460

cuuaacaaac uggaugacuc cgaucucaga ucucgcaagc uguaccugua uuacacacag     2520

auggggcggg acauguauac gggagagaaa aucgaucuug acaaacucuu cgauagcacc     2580

cauuacgaca aggaucacau uaucccccag uccaugaaga aggacgacag cauuaucaau     2640

aaccugguuc uggugaauaa aaacgcgaau cagacaacca agggcaacau uuacccgguc     2700

ccuucuucca ucagaaauaa ccccaaaauc uauaauuacu ggaaguaucu cauggaaaaa     2760

gaguuuauua gcaaggaaaa guacaaccgg cugauccgca acacaccucu uaccaaugag     2820

gagcuugggg gcuucauuaa cagacagcuc guggaaacgc ggcaaucuac caaagccauc     2880

aaggagcugu uugaaaaauu cuaucagaag uccaaaauua uccccgugaa ggcuagccug     2940

gccucugauc uuagaaagga caugaauaca cugaaaagcc gcgagguuaa cgaucuccac     3000

caugcucacg acgccuuccu gaauauuguc gccggagaug uguggaaccg ggaguuuacc     3060

uccaauccca ucaacuacgu gaaggaaaau agagagggcg acaaagucaa guauagccuc     3120

ucuaaagauu ucacacgccc uagaaagucc aaggggaaag ugaucuggac cccggaaaag     3180

ggacggaaac ugauuguuga cacgcuuaac aagcccagcg ugcugaucuc caaugagucu     3240

caugucaaaa agggcgaacu cuuuaacgcu acaauugcgg ggaaaaagga uuacaagaaa     3300

ggaaagaucu accugccucu gaaaaaggac gaucgccuuc aggacgugag caaauauggc     3360

ggguacaagg ccauuaaugg cgcuuucuuu uuccugguug agcacaccaa guccaaaaag     3420

agaauccggu cuaucgagcu cuuuccccug caucuccuga gcaaauucua ugaagauaag     3480

aacacagugc uugacuacgc cauuaauguc cugcagcucc aagacccuaa aaucauuauc     3540

gauaagauua acuaucgcac cgagaucauc auugacaauu uuuccuaccu gaucucuacc     3600

aagagcaacg auggauccau uacagugaaa cccaacgaac agauguauug gagaguugac     3660

gagaucagca aucugaagaa aauugaaaac aaguacaaaa aggaugccau ccuuacggag     3720

gaggaccgga agauuaugga aucuuacauc gauaaaaucu aucagcaauu caaggcuggc     3780

aaauacaaga aucgcagaac cacagacacc auuaucgaga aauaugaaau uaucgaucug     3840

gacacacucg auaacaagca gcuguaccag cuccuggugg ccuuuauuuc ccuuagcuau     3900

aaaaccucua auaacgcggu cgacuucacg gugaucgggc ugggaacaga guguggcaag     3960

ccgcggauca ccaaucugcc ugauaacacc uaccucgugu auaaguccau uacagggauc     4020

uacgaaaaac gcauuagaau caaguga                                         4047


<210>  4177
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4177
auggagaaga acuacuaucu gggccucgac cuggggagca auuccguggg augggccgcu       60

accgaugaag acuacaacuu ccuuagacug aaaggcaaga cagccugggg agcgcggauc      120

uuuucugagg cuaaagauug caaggaacgc agaacguauc ggagcaauaa acgcagacuc      180

gcccggcgca aguacagacu gguccuucug aagcagcucu uugccgagga aaugaccaaa      240

guugacaaga cauuuuuccu gcggcuggcu aacuccaccu auugggagga ggauaaaaca      300

aaggacucug augggaauga aauuggcaaa aaccucaucu uuaagaccca cgaggaagag      360

aagcaauacu auacaaaaua ccccaccauu uggcaucugc gcaaggcccu uaucgagaau      420

gacgaagagg cucugagcaa ccucagauac guguaucugg ccauucacca uaucgugaaa      480

uaccggggga auuuccuuac ggaaggagag augggcaacg auaucccucc gauuaccaca      540

aucgaagaga uuaaucagac ccugucccuc cuggaggucg acucuagcga agaugcgaca      600

gacaagacca cguauaucaa acccgagucc uacaacgaac uguuuacaau ucucgagaau      660

aacaaucuga acaaggugaa aaagcagcag gaaaucgaua agcuguucau uagcucuaaa      720

gagcgcuccg agaacaucaa gaauuauauc aaccuguuua aaaccauuau cguuggcggg      780

agcuucucuc ucaagaaaau uaaugaagac acagaggaca ucuccgugga uuuuaccaag      840

gacuucgaaa acagcgaggc cgauauuucu aaggcucugg gagacuccuu uaaucuuguc      900

cugaucgcca aaaaguacua ugauuacauc auucucaaag aacugcuggg caagaacaaa      960

gagaucagcg aggcuauggu gcaaauuuac gaagaacaca aggacgaucu caagucccug     1020

aaaaccaucc uuaaggaccu ggauaauaaa cucgggaacg acggaaagga uagaauuuau     1080

uacucuguuu ucaaacagga accuacagag aaguucaagg agacgaauua ucugagcuuu     1140

gugcagggcg ggaaacuuga agaacugaac aaguacauca aaaagauccu cgaaaauaac     1200

gagaaagaca uugacgauaa ggaaaccuau aaugcccugu acgagaaagc guguaaggga     1260

gaccuguuca agaaacaggc caacgcuugc acaucccuca ucccccauca auuucaccuu     1320

cgggagcuua agaaaauucu ggaaaaugcc ggccgcaagu auagcuuccu caacgauauc     1380

ucugacaaaa uuaucaagau uuuuaaguuc agagucccuu acuacuaugg cccccugaac     1440

aaugagucca acgcucacag cuggguggug aaaaaggaag ggcaggagaa agccaagauc     1500

accccuugga auaucaacga aauuaucgau acaccgaaaa cccgggacaa uuucuucaag     1560

aagcugacga acucuuguac auaccuuguc ggagagaccg uucuccccaa aaccagccug     1620

uauuacgaga aguaucuggu gcucgauaga cugaauaaaa ugcaagugaa cggcgacuac     1680

cuuuccgccu cugaaaagaa ugauguccug aaauuuauuc ucagccgcaa gacaaccaca     1740

aucgagcaga uucggacgua ucuguccaag aacaaaagca ccaaggcuaa ugacaucucu     1800

aucuccaaca uuaaagcgac agauccuuuc aaugccguga gccaugcuuu uuacgggaag     1860

caccccgacc ugaacaucaa ucuuugcgag gagcucauua aauucgccac caucuacgcc     1920

gaugacaaga aagaacugaa gaaguuuguu ucuuccaacu ucaaccugac agacgaguuc     1980

auuaaagagu aucucaauac cccuacgaac aaguggggaa gacugagccg caaauuucuu     2040

uguggcgggg aucagcugga aauguacuau guggacgaua auggaauccu ccauuccauc     2100

cugaacauuc uggaggaaac caaugagaac cuucaggaga uccucaauaa cgaaaaguac     2160

ggcuucaauc agaaaauugc ugacuuuaac aauacacuga ccgccgauaa gacaaccgag     2220

gaacaaaucg aggacauucu ggauucuacg cccgcgcuca ugcggagaag caucauccag     2280

acacuggcug ucguggacga uguuauuaag gccgccggca aagcuccgca gaagaucaug     2340

auggaaguga cccgcacaga cgggaaaaag gaggagacca aaucccggua uguccagcuu     2400

aagaagcaaa uuaccaacga uaaagugcug acaucugacg aaaagaaaga gcuccugacg     2460

aaucuggaaa acaagaaaau ccagcuuaag ggaaagcaca uuuaccucua uuucaugcag     2520

augggcaucg auuuuuacac cgggaaaauu auggacauca augacgucau caacggaaag     2580

uacgauauug accacaucau uccucagagc augaucaaag augacucccu ggauaacaug     2640

gugcugguua gcaaggacua uaaucaaaaa gugaagaagg auauuuaccc caucugcgac     2700

gagaucagaa acaauaugcu cgauaaaugg aagggcuauu acgcccgcaa aauuaucucu     2760

gaagagaagu auaaccggcu gaaaagaaca aaggagcuua augcugacga aauuaacgcc     2820

uucaucaauc gccagauuaa cgucgugaau uacuccaaca uccagaucaa acggauuuuu     2880

gagaucaagu auccugaaac caagauuauc uucagcaaag cgucuuuucc cucccugcuc     2940

agaaaggagu acgaaauugc caaaaugcgg gaucugaaug acgcucauca cgccguugac     3000

gcuuaccuga acaucgugug ugggaauauu cuuacaaccc uguauacgga uaucuucuac     3060

auguuuaagg agaaaaagga ggaccauaag aacaucagcu ucaauaugga aaaacgccuc     3120

auuaacuaua ucgauaagaa uaaccugaaa gacaagauua aaacaucuug ccucagacac     3180

gaugcccuga ucaccuacaa gcccgacuau gauagcggca aguuuuacaa agagaccauu     3240

uucaagaaau ccggagacaa ggaaacaggc cuuaucccuc ugcauaccaa agggccgaug     3300

agcgauacaa agaaguaugg aggcuacucu cagcucuccc aaagcuacau cauugcgguc     3360

gaguauaaac ugaagaacaa aacggugaag aaacuguguc gggugcccac ccuuuaccug     3420

aagcaguaua aaaaugaccu caacacaauc gccaagauua ugaucgauga cgagaaggcu     3480

accgauauua aaguucugag aaagaucuac cucaaucaga aaauccgcua uaacgggugc     3540

uacuaucuga uuuacacauc caaggaaaaa cagaauaagu acaagauggc cuaucaaaac     3600

uaccuugaca augacuaucu gaucuaccuc aacaaauguc ugaagaaaau ugaugagcug     3660

gacgauggag aacuuaccuc ucaggaguuc aucgucaaua aggacggcga uaaauuucug     3720

auugacaaga accacaaucu cgaaaucuuc aagagcauua aagagcagua uaacaagacg     3780

aucuacgauu auaccccuua caucgugcaa ucccgggaga ugaaagaaga ggacuuuaau     3840

aaccugucuc ucaagaaaca gauugauaca cugcuuaaua ugaucaagau ucugagcaga     3900

uccaacgaac aggcuaaguu cgagaccgcc uuuuucgaaa acuuuacaaa aguguuccug     3960

aagaccauga caaucaccaa ugagaaaguc uacauuguga acgagucucc cagcgggcuc     4020

uuuaagggca aagaggagcu gaucuga                                         4047


<210>  4178
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4178
augaagaaau ucgaggacua cuaucugggc cucgaucugg ggaccagcuc caucggaugg       60

gccgugacaa acgaaaagua cgacauucuu agauuuaaua aaaaguucau guggggcacg      120

caccuguuug auauggggaa caccgcuucu gagcggcgca ucuucagaac aagccggcgc      180

agacucaaac ggaagaagaa ucgcauugaa cugcuucaga cccuguuuuc cgccgagaug      240

ucuaaaaagg acuucggauu uuuccaaaga cucaaagaaa gccaguauuu uaucgaggau      300

aagacauuca aaaacaagaa uacccuguuu auggacgaga acuacacaga ucaggacuau      360

cauauggaau accccaccau uuaucaucug cggaaggcgc ucaucgauaa ugaggaaucc      420

gcuuucgaga uccgcaaacu guaccuugcc auuucucaca uccugagcca cagaggccau      480

uuucucuucg agggggaacu guccgacaac cuugauuucg acgauauuuu uaaugaguuc      540

gucaaguacc ugaacgaaga acucaauauc gacuuuaaaa uuaagagcga cguuucucug      600

caggccauca aagaaauucu gacggauaag augcucaacc ggaccaaaaa gaaggagaaa      660

cuguccgagc uucuggaaga ggaaaagaau aaaaucuauc aagagaucug gaagcuccug      720

gugggaaacg aagcuaaaau gagcacaauu uucgacgaua aggaguacga ggaagccaag      780

aucgcuuuuu cuaaaggcaa uuaugacgag aaggaagagg aauacgaguc cauucuuggc      840

gagaaaaucc uguucgugca gaaggccaaa gcgauuuaug auuggaugcu ccugcagaac      900

auccugaagg aagacaagua ccucagcuau ucuaaaguca agcuguacaa cgagcacaaa      960

aaggaucuua aaauccugaa ggacgugcuc aagaaucugg auaaaaagau uuacaaagaa     1020

aucuuuuccg agaacgggcu uuaugaggaa uaccugcgca uugagaagaa aaagaucgga     1080

agcaaagaag agaagaccaa ggacauucuc uauaauagac ugaaaaagau cuucgaaaac     1140

cgggauaaua uggccaucga ggacauucug aacaaaaucg agcucgacuc cuuucugccu     1200

aagcagaaaa uuaaggauaa uggcguuauc ccgcgccaag ugcaccagaa ggaacuugag     1260

auuauccuga aaaacgcuga aaaguacuuc aaauggcuca uugagaagga cgaaacaggg     1320

cugucuguca gcgagaaaau caugaagauc uuuaaguuca aaauucccua uuacguggga     1380

ccucuuaauu ccaagagccc cuuugccugg aucgagagaa aagaauaugg caagauucuu     1440

ccuuggaacu ucgagaauau gguugauaug ucuaaaaccg cugaaaaguu caucaagcgg     1500

augacgaaca aaugcacaua ccuccccgag aaggacguga uuccuaaaaa uucccugcug     1560

uacaccaagu auaugguccu caacgaacug aauaaccuua aaaucagagg ggagaagauc     1620

aaaguggagc ugaagcagaa gauuuacaca gaacucuuuc agaaaaacaa gaaaaucgga     1680

augaagaccc ugauugaguu ccugaaaagc caaaauaucg uggucaccaa gguugaaauu     1740

acaggcaucg auggcacguu uaacaccucu cuugggagcu auaucgacau gaagaaaauu     1800

uucggagagg agaagaucgu gaaagauucc uacaagaaaa ugauugaaga caucauucuc     1860

uggaucacac uguauggcga ggaaaagaag cugaucaaau cuaagauuga ggaaaaauac     1920

ccggagaagg ugacccccga ggaaaugaaa cucauccuga aacuuaagua ucgcgauugg     1980

agccggcugu ccaaagaguu ucucacagaa auugagacca uggacgaaga gacgggggag     2040

cugaucagca uuaucgaaau gcuguggaga aagaaugaga acauuaugga acuucucucu     2100

uacaaauacg auuucuccaa ucgcaucaag gaguauaacg gaaucaauga aaaagagaag     2160

aacauuacau acgaggaccu ggucaaggau cugcagauca gcccuucugu gaaaaagaug     2220

auuuggcggg cccucguuau cgugaaagaa auuaagaaag ucaccaauaa gucccccaaa     2280

aagguguuua ucgagauggc gagagacacc cagacagaaa agaaacgcac cgagaagcgg     2340

aaagacaagc ugcuugagcu guauaaaaac aucaagaaug augacagcau uaaggaacuc     2400

gagaaaaacc ugaaggauaa aacaaaugac gaucugaagu ccaaaaagcu guaccucuau     2460

uaccugcaac ugggcagaug uauguauucu ggggaaguua uccgccucga ggaccugaug     2520

aacgauaauc uuuacgacau ugaucauauc uacccucaga gcaagauuaa agacgauucc     2580

uucgacaaca cggugcuggu caagaaacag aucaacgccg auaagaccaa ugaauauccc     2640

aucgacaaau cuauucagaa caagugcaag gcuuuuugga caagccucaa agagcgggga     2700

cugaugucca auaccaagua cgagagacug acacgcaccg aaggcuucag cgacgaugag     2760

cuugccggcu uuaucucucg gcaaauugug gaaacgagac aggcuaccaa aguccuggcc     2820

gaccuccuga aggugaucuu cggguccgau acacggauug uuuaugugaa agccggacuc     2880

accagcgacu uucgccagaa guucggcauc uacaaauuca gagagcugaa cgauuaucac     2940

caugcucacg acggguaccu uaauauuguc acaggaaaca ucuauaauac ccuguuuacg     3000

gauaacccgu acaauuucau caaggaccgg aagaaagaua acagaacaua caaucucaac     3060

aagguguuug aacgcgagaa ugacguuugg aacccugaug ugaugcuguc uaaaauugag     3120

cgguauaucu acaagauuag accccuguuc acccgcgcgu ccuaugaaca gaaaggcggg     3180

uuuagcgacc aaaauaucuc uagcgagaag gacaaacuua gaccucugaa guccagcggc     3240

guccucucua acauuaagua cggaggcuau gauaaaauca agaacuccua cuucuuuauc     3300

guggaauaua cagagaaaaa gaagcgggug cgcagcauug aaacccugcc caucuacauu     3360

aaaaagucca ucucuaccaa agaggaccuc gagaaguacu guaaagagga gcugaaacuu     3420

caggaaccca agguucuggu cagcaagcuc aauuauaagu cccugauuua cucuaacggg     3480

cacgaguauu acaucaauag caaaacagcc gauuccauca cgauucagaa cggaauccag     3540

auuauccuga ccaaggagaa uuaugaaaca uacagaaaaa uuuauaagaa ccuugaguac     3600

cugaaacuca ucuacaagga cgaaaagauu cgggaggaaa agauccugga gaagaucccu     3660

aaaauucuca agaauaacaa aaaugaucgc gaccugucug aggaugaaau cagcgagaag     3720

uucuggaaac uuuauaagga aauuuuugag aagaucaaaa uuggcaucuu cuccaaguuu     3780

aaaagcaccc ugccgaucua cauugaagac ugcaagaagc ugaaagauuu cacaacccuc     3840

aacaauuaug cuaagucuag auucauccaa gagauucuga aaugucuuaa cucccagcag     3900

ggggaguuug acacgaagua cacacauaau uauagcaaaa aguacaacac caagcugucu     3960

aaauccaaga uccucgaaaa ugauuucuau auuaucaacc agagccccac cggacuguuu     4020

gagaaaaagg uguuccucga caaauga                                         4047


<210>  4179
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4179
augggcgagg acgggaagcc ccugagagug aaaggaaaga ccgccaucgg cgcuagacuc       60

uuccaggaag ggaacccugc cgcggaucgc agaauguuuc ggacaacgcg cagacggcug      120

aaucgcagaa aauggcggcu uaagcugcuc gacgagauuu ucgauccgua caucaccccc      180

gucgacagca cauuuuucgc ucgccugaaa caauccaacc uuucuccuaa ggauagcaga      240

aaggaauuua aaggauccau gcuguucccc gaccucaccg auaagcagua ucaccuggac      300

uacccuacaa uuuaucaucu gcggcacgcc cucaugaccc aggauaaaca guuugacauc      360

cgcaugguuu accuggccau ucaucacauc gugaaguaua gaggcaauuu ccuuaacucu      420

acacccgugg auagcuuuaa ggcuuccaaa gucaauuucg acgaucaauu uaagaaacug      480

aacgagcucu acaccgccau caauccugaa gagucuuucc agauuaaccu ggcuaauagc      540

gagaagaucg ggcagcaguu ucuugacccg uccauucgga aauucgacaa gaaaaagcaa      600

auccccaaaa uugugccugu uacgauggau gacaaggccg uggauaagcu gaacggaaaa      660

aucgcgagcg aaauuaucaa ugccauccuc ggcuauaagu cuaaacugga cgucgcugug      720

cagugcgauc ccgaggacuc caacccuugg gccguuaagu ucgaugacga agauauugac      780

gcuaaacugc agaagauccu ccccgagaug gaugagaauc agcaaagcau uaucggcauu      840

auccaggacc uguacucuca ggugacccuu aaccaaaucg ucccgaaugg gaugucccug      900

agcgaaucua ugauuaagaa auacaacgac caucacgauc accucaagcu guauaaaaag      960

cugaucggac agauuuccga cagcaaaaag aagacagccc uuaaaaaggc guacgaucag     1020

uaugugggca acgacgggaa agugaucgag caggaugacu uuuacuccuc ugucaagaaa     1080

aaucucgaug acagcgaacu guccaagcaa auuauggauc ugaucgacgc cgauaaguuc     1140

augccuaaac agcgcaccag ccagaacgga guuauccccc aucagcucca ccaaagagag     1200

cuggacgaaa uuaucgagca ucagucuaag uauuacccuu ggcuugcuga guccaauccc     1260

aacaaacacg aucugcaccu cgccaaguau aaaauugaag aacugguggc uuuucggauc     1320

cccuacuacg ugggcccuau gauuacaccg gaagaccaga agaaaagcgg ggccgccguc     1380

uucucuugga ugaagagaaa ggagcccgga cagaucaccc cuuggaauuu ugacgaaaaa     1440

guggaucgca uggagagcgc uaacaaguuc auuaaacgga ugacgacaaa ggacaccuau     1500

cuuaucggcg aggauguucu gcccgacgaa ucccuccugu acgagaaauu uaaggugcug     1560

aaugaacuca acauggucag agugaauggc gauucucuga agguugcgga caaacaagcc     1620

aucuaucagg aucuuuucga gaacuacaag cauauuagcg ugaaaaagcu gcagaauuau     1680

aucaaaggga agacaggacu cccuaacaau cccgaaauuu ccggccugag cgacccggau     1740

caguuuaaca auucucuggg gaccuacaac gacuucaaga aacuuuuugg agauaagguc     1800

gacgauccug accuccaaaa cgacuucgag aaaaucgugg agugguccac cgucuuugaa     1860

gauaagaaaa uucugcgcgc uaagcugaau gagaucacau ggcucacgga caagcagaaa     1920

gaugaccugg aaagcucucg guaucagggc ugggggagac uuuccaagaa acugcucacc     1980

ggaaucguga acgaucaagg cgagcgcauu aucgacgagc uguggaauac aaacaagaau     2040

uucaugcaga uucagagcga ugacgauuuc gccaaacgga uccacgaagc caacgcugac     2100

cagaugaagu ccaccaauau ggaggauguu cuggccgacg cguacacauc uccccaaaac     2160

aaaaaggcua uuagacaggu ggucaaggug guugacgaua uccagaaagc caugggcggg     2220

guggccccua aguuuaucag cauugaguuc acccgcuccg aggaccggaa ucccagacgc     2280

acgaucucuc ggcagagaca acuugaaaac acacucaaag auaccgcuaa gagccugguc     2340

aaauccauga auccuaacau ucugagcgag cucgacaaug ccucucgguc caagaaggga     2400

cugaccgauc gccuuuaccu guauuuuaca cagcucggca aagacaugua caccggggag     2460

cccaucaaca uugaugaaau cagcaauuau gacaucgauc auauucugcc gcaggcuuuc     2520

aucaaggacg auucucuguc caacagagug cuugugagca aagccguuaa caauggaaag     2580

ucugcgaacg ucccucucaa acuguuuggc gccaagaugg ggcacuucug gaagcagcug     2640

gcugaggccg gccucauuaa uaaacauaag cugaaaaacc uucaaacaaa ucccgacacg     2700

aucagcaagu uugcuaugca cggauucauu cggagacagc ugguggaaac cucccaggug     2760

aucaaacucg ucgccaacau ucugggcgau aaguaccgca augacgacac aaagaucauc     2820

gagauuaccg cgcggaugaa ccaccaaaug agagaugaau uuggguucau caaaaaucgc     2880

gagauuaacg acuaucauca cgccuuugau gcuuaccuga cagccuuccu uggaagauau     2940

cucuaccauc gguacaucaa gcugcgcccc uauuuugugu acggcgacuu caaaaaguuc     3000

agagaggaua aaguuaccau gcggaauuuu aacuuccugc acgaccucac ggaugacacc     3060

caggcucgca uugccgauac agaaaccggg gaggugaucu gggacagaga aacaagcauc     3120

aagcagcugc gggaugucua ucacuacaag uuuaugcuua uuucucagga gguguauacc     3180

cugcgcggag ccauguucaa ucaaacgauc uacccugcuu cccaggcggg ccggaaaaag     3240

cucauuccga ugaaagccga caagcccguu gauguguaug ggggcuacag cggauccaca     3300

gacgcuuaca uggccaucgu ccggauucau gacaaaaagg gcgauaaaua uaaggugguu     3360

ggggugccua ugcgcgcccu gaacagacug aauaccgcua agaacgucuc ugacgccaaa     3420

uacgaucagg aacuuaagga ggugcugaca ccccaguuua ccaaaagcaa gaaaaaccgg     3480

aagaccggag agaucacaca aacgguggaa gacuucgagg ucguucucgg caaggugaug     3540

uauagacagc uggugaucga uggggacaaa aaguuuaugc ucggauccuc uaccuaccag     3600

uauaaugcga aacagcuggu ccuuagcgau gaauccauua agacacuggc uucugagggc     3660

aaacucaacg aggcccaaga aagcgccgcu uacgacaagg uguaugauga gauccuggac     3720

aagguuaaua aauacuucgc ccuguacgau accaacaagu uucgccagaa acuuucccug     3780

gggaaggaca aauucguggg ccucccuaau cacaacgucu uugacggaag caagaagauu     3840

ucuuccggca aacgggaaau ccugaaugag auucucaacg ggcugcaugc gaaugcuaca     3900

cuuggaaccc ugagcgauau cggcuucucu acgcccuucg ggaagaugca gcagccuaga     3960

ggaauuggcc ucuccagcaa cacaucuauc gaauaugagu ccuuuaccgg gcuguucaaa     4020

cgcaccaucu uucugaagga ccuuuga                                         4047


<210>  4180
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4180
augaagaaac ccuacaccau cgcccuggac auuggcacaa acagcguggg gugggucguu       60

gugacggaug acuauagagu gccuaccaag aaaaugaagg uccucggaaa uacagagcgg      120

aaaaccauca agaagaaccu gauuggcgcc cuucuguucg auuccgggga cacagcugaa      180

ggaacccgcc ucaaaagaac agcgcggccg cgcuacacca gacggaagaa ucgccugaga      240

uuucuuaaag agaucuucac ggaagagaug gcuaaggugg augacggcuu uuuccagcgg      300

cuggaagauu cuuuuuaugu ucucgaggac aaagagggga acaagcaccc cauuuucgcc      360

aaucuggccg augaaguggc uuaccauaag aaauauccua ccaucuacca ucugcgcaag      420

gagcucgucg acaaccccca aaaagccgau cugagacuua ucuaucuggc uguggcccac      480

auuaucaagu uucggggaca cuuccucauu gaaggcacac ugagcuccaa aaauaacaau      540

cuucagaagu cuuuugacca ucugguugau accuacaacc uccuguucga ggagcagcgc      600

cugcucacag aaggcaucaa ugcgaaagag cugcuuagcg ccgcucuguc caagucuaag      660

agacucgaaa accugauuag ccuuaucccu gggcagaaaa agaccggaau uuuuggcaau      720

aucaucgccc ugucccucgg gcugacgccc aacuucaaag cuaauuuugg acugagcaag      780

gacgugaaac uccaacuggc caaggacaca uacgcggaug accuugauuc ucugcucgcc      840

cagauuggcg accaguaugc ugaucuguuc cuugccgcua agaaccuguc cgacgccauc      900

cuccugagcg auauucugac cgagucugac gaaaucacac gggccccucu cuccgcuagc      960

auggucaaac gcuacagaga gcaccacaag gaucugguga cccuuaaaac ccugauuaag     1020

gaccagcucc cggagaaaua ucaagaaauc uuccuggaua agacaaagaa cggguacgcg     1080

ggauauaucg agggccaggu gucucaggaa gaguuuuaca aauaucugaa gcccauucuu     1140

gcccggcucg acggcuccga accucugcug cucaaaaucg acagagagga uuuccugcgc     1200

aagcagcgga cguuugacaa ugggagcauu ccccaucaaa uccaccuuga ggaacugcau     1260

gcuauucuca gacgccagga ggucuucuac ccuuuucuga aagauaaccg gaagaagauc     1320

gaaucccugc uuaccuucag aauccccuac uauguuggac cgcucgcccg cggccacucu     1380

cgguuugccu gggugaaaag aaaguucgac ggggcuauuc gcccuuggaa uuuugaggaa     1440

aucguggaug aggaggccag cgcgcagauu uucaucgaaa agaugacaaa gaacgaccug     1500

uaccugccca augagaaagu ccucccuaag cacucccugc uuuaugaaac cuuuacagug     1560

uacaacgagc ugaccaaagu uaaguaugcu acggagggaa ugacacggcc ccaauuccuc     1620

agcgccgauc agaagcaggc cauuguggac cugcuguuua aaaccaauag aaaggucacc     1680

gugaaacagc uuaaggaaaa cuacuucaaa aagaucgagu gcugggauuc uguugaaauu     1740

acaggcgugg aggacuccuu caaugcuagc cucgggaccu aucaugaucu gcugaagauc     1800

auccaagaca aagauuuucu cgacaacccc gacaaucaga agauuaucga agauauuauc     1860

cugacacuua cgcuguucga ggacaaaaag augauuucua aacgccucga ucaguacgcc     1920

caccuguuug acaaggucgu gcugaacaaa cuugagcggc aucacuacac cggauggggc     1980

agacucagcg gcaaacugau caaugggauc cgggauaagc aguccggaaa aacaauucug     2040

gacuuccuca aggcugaugg cuuugccaac cgcaauuuca ugcaacugau ccaugacucu     2100

gaacuuagcu uuauugauga gaucgcgaaa gcccagguca uugggaagac cgaauauucc     2160

aaggaccugg ugggaaaccu cgcuggcagc ccugccauca aaaaggggau cucucagaca     2220

auuaaaaucg uugaugagcu ggugaagauu augggcuacc ugccgcagca aaucgucauu     2280

gaaauggcua gagagaacca gaccacggcc cagggaauca aaaaugcgcg gcagagaaug     2340

cgcaaacuug aggaaaccgc caagaagcug ggcuccaaca uccucaagga gcaccccgug     2400

gacaauagcc aacugcagaa cgauaaacgg uaucucuacu aucugcagaa ugggaaggac     2460

auguacacag gagacgaucu ugacauugau uaucugucuu ccuacgacau cgaucacauu     2520

aucccucaga gcuucauuaa aaacaauucc aucgacaaca agguucucac cucucaaggc     2580

gcuaauagag ggaaacugga uaacgugccc agcgaagcca uuguccgcaa gaugaaggga     2640

uacuggcagu cccugcuuag agcuggcgcc aucucuaaac agaaguuuga caaucugaca     2700

aaagccgagc gcgggggccu cacccaagug gauaaggcug gauucaucca gcugcagcuc     2760

guggaaacgc ggcagauuac aaaacauguu gcgcaaaucc uggacagcag auuuaacacc     2820

gaguucgaug accacaauaa gcggauucgc aagguccaua ucauuacacu uaaauccaag     2880

cuggugagcg acuuuagaaa agaguucggc cucuauaaga uccgggauau caaccacuac     2940

caccaugccc acgacgcuua ucugaaugcc guggucgcca aagcuauucu ggggaaguac     3000

ccucagcuug cccccgaguu cguguaugga gauuacccga aguauaacuc uuuuaaagaa     3060

cgccagaagg cgacccagaa aacccuguuc uacuccaaca uccucaaguu uuucaaagac     3120

caagaaagcc ugcauguuaa uucugauggc gaggagauuu ggaacgcuaa uaagcaccuc     3180

ccuaucauua agaacgugcu guccaucccc caggucaaua ucgugaaaaa gacagaaguu     3240

cagaccgggg gauuuuacaa agagagcauu cuuucuaagg gcaacagcga caaacugauc     3300

ccuagaaaga auaacuggga uacgcggaaa uaugggggcu ucgacucccc cacaguggcc     3360

uacagcgucc uggugauugc caagauggaa aagggaaaag cuaagguucu caaaccugug     3420

aaggagaugg ucggcaucac cauuauggaa cgcaucgccu uugaggagaa ucccguggug     3480

uuccuggaag cgaaagggua uagagagauu caagaacauc uuaucaucaa gcugccgaag     3540

uacucucucu uugagcugga aaacggacgg agacgccucc uggcuuccgc cagcgagcuu     3600

cagaaaggca augagcuguu ccugccuguc gauuauauga cauuucucua ccuggccgcu     3660

cacuaucacg aacuuaccgg guccucugag gacguucugc ggaagaaaua cuucguggaa     3720

agacaucucc acuacuucga ugacauuauc cagaugauua acgauuuugc cgagcgccau     3780

auccuggcua gcuccaaucu cgaaaagauu aaccacacgu aucauaauaa cucugaccug     3840

cccgugaacg agcgggccga gaauaucauc aacgucuuca cauuuguggc gcuuggagcc     3900

cccgcugccu ucaaauuuuu cgacgcuacc auugauagaa agcgcuacac cagcacaaag     3960

gaaguucuga augccacccu gauccaccag uccgugacag gccucuauga gacgcggauu     4020

gaccugucuc aacuugggga aaacuga                                         4047


<210>  4181
<211>  4050
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4181
augaagcaga aauucuacgu gggccuggac auggggaccg ccagcgucgg augggcuguu       60

acagaugaca aguauaacau cauuaaaaga cacggcaagg cccucugggg agugcggcug      120

uuuaaaacgg cgaauaccgc ugaggaacgc agaaucuucc gggccgcccg cagacggaca      180

caacgcagaa agcagcggau ggagcuucug caggaaauuu uuggagauga gaucuccaag      240

accgacauug gcuucuacca gcgcaugaaa gaaucuaagu auugguuuga ggauaaaaga      300

gacaucaacg ggaagcaacc cgagcucccu uacgcucugu ucguggauga cggauuuaca      360

gauaaagacu aucaugaaca guucccgacc aucuuucacc uucggaaugu ccuggugaac      420

gaguggacac agaaguacga uauucgccuc guuuaucugg ccauccauca cauuaucaag      480

cauagaggcc acuuccuguu ugaaggcaaa aagaugagcg aggugaccga cuucgggucc      540

gcuuuugagg ccuucugcaa agucgcgucu gauaaugaca uugaaaucac gguggacgag      600

gaaaagcuca aagauauuga ggaccugcuu aagagccggc agaaauccaa gaccgccaag      660

aaacaagaac ugggaaagau caucgcuggc gccgauaaac agaagaaagc ucucgccgcg      720

cugguuacag gguguauggu gaaacuuucu gagauuuucg ccgacaagag ccuggaugac      780

ggagagcgca acaaaaucuc cuuugcugau agcaauuacg acgaauacau ugccaacguc      840

gaggaaaccc ucgcugagag auuccugcug aucgccgccg gcaaggcucu cuaugauugg      900

gcggugcugu cugacauucu ugaagggucc acauacauca gcgaggccaa agugaagauc      960

uauaaaaagc acggagauga ccugcggaag cucaaaggcc ugcuuaagac cagacccgac     1020

gaguacaaac ugauuuuugg ccucccugaa aagcaggcua auuauucugc cuacaucggg     1080

auggucaaaa agaacggaaa gaaaguuccc auugauaaga aaugcacgaa ggccgacuuc     1140

uauuccuuuc ugaaaaagac acugaauaaa aucaacuucg aggaugaaaa ggagcgcgaa     1200

auugcugaga gcaucaucaa ugagauugaa aacgagaccu uucucccuaa acaggugauc     1260

ucugacaaug gcgugauucc cuaccaacug caugaagccg agcuugaucg gauccugaag     1320

aacgcggcuc ucuacuaucc uuuccugucc gagaaggacg augaagggua cacaaacaug     1380

gacaaaauua aggccaucuu uaaauuccgc auuccguauu acgucggacc ccuuaauacc     1440

uaucacagcg auaagggcgg gaacuccugg gccgugagaa agcaggacgg aaaaaucuac     1500

ccuuggaauu uugagucuaa gaucgaugcu ggcgaaagcg cccggaaauu cauuggcaga     1560

augaccaaca aguguacaua ucugaucggg aaagacguuc uccccaagga gucccugcug     1620

uacagcaagu uuauggugcu caaugaacug aacaaucuuc gcauugaugg agagaaaguc     1680

ucuguggaga ucaagcaggc uguuuacgaa aacguguuca aaaaguauca acgggucacg     1740

ggcaaaaagg ugaaggacuu ccugauuaga uccgggauca ucggacgcga gcaggaacuc     1800

agcggcuuug accagaauuu caaaucuagc cugaaggccu accaugauau uaaacagauc     1860

gcgggggaca uuaaccuguc ccaaaaucag aaggaggaua ucauuaaaga aaucaccguc     1920

uuugccgacu cucagaagau gcuuaaaaag cggcucaaga ucaaauaucc ugagcugagc     1980

gauaaacagg ugacaaagcu ggcugccaag aaauacaccg gcuggggaag acucucccgc     2040

gaguuccugg aagagguuga aagcgugaac aaggagacag gcgaaguccu uaauauuauc     2100

ucugcucugu gggagaccaa cgagaaucuc augcaacugc uguccaacaa guaugacuac     2160

cuuagcgaaa uuaaacggcu caacgaugag gccgaagaca agucugaggu gguuacguau     2220

gaugacgugc agaaacugua cgucuccccc gcggugaaga gaccggugug gcagacacug     2280

aaaaucguug aggaaauuag caagaucaug ggguccaagc cugagcggau cuuugucgaa     2340

auggcccgcg agcccggaca gaaaggcgau gugaaggugu cuagaaaaag ccggcuccug     2400

gaaaaguaca aauauugcaa gaaggaggcu ccugagauuu acgaaaaucu ugucaaagag     2460

gacgauaaca agcugagauc cgacaaacuc uuccuguauu acacccaaau ggggcgcugu     2520

auguauucua augaagugau cgacauugag gaucuguuua ccaaccggua cgacaucgau     2580

cacauuuauc cccagagcaa gguuauggac gauucccuug acaauagagu gcucgucaaa     2640

cgcgaacuga acagcaauaa gacagaugug uuccccguuc cuaaggagua cauggacaaa     2700

gccaagaccc ugugggauau gcucaaaaag aaagaccuga ucucuaagga gaaauaccac     2760

agacuuacac ggcgcgaugg auuuuccgaa aacgagcuga gcggcuucau ugcuagacag     2820

cucguggaaa cgcggcaguc uaccaaggcc gucacacaac ugcugaccaa ggccuauccg     2880

gaguccgaaa ucguguacgc uaaagcgaau gccguuagcg aguuucgcca uaaguucggg     2940

uuuacaaaag ugagaucuau caacgacuau caccaugcua aggacgccua ccuuaauauu     3000

gucgugggca acaccuauua cgugaaauuc acgaaggaug ccgcuugguu cauccggaau     3060

aaccccggac gcaccuauaa ucucaagaaa auguuugagc agggcacagu cagcagaggg     3120

ggagaaaccg ccuggguuuc cggcgaggac gggacaauuu ucaccgugaa gcgggugaug     3180

gggaaaaaca acauccuguu uacgcgcaag gcguacgaag ucagaggcgg gcuguucgau     3240

cagcaaauua ugaaaaaggg caagggacag aucccuguga aaaauaacag cgaggacgau     3300

cggcucugcg cuacagagaa guacggcgcc uauaauaaag ccucuggggc uuacuuuaug     3360

cugguugaau ccgaggacaa gaaaggaaac aagaucagaa cccuugaguu cgugcccguc     3420

uaucuggcca aggagcucga aaagagcgag gaugcggcua uucgcuacug uguggagaag     3480

cuggaacuga aaaauccuga cguucgggug agaaagauca aaauggauuc ccuuuuuaac     3540

gucgacggcu uccgcaugca ccugucuggg cggacaggaa agcagcucau uuuuaaaugc     3600

gccgagcagc uggugcucga ugaagagagc caaaagaucc ugaagaaaac cgccaagguu     3660

uucgcugacu auaaagaaaa uaagaacauu aaaaucuccg auaagaucga gcuuaccgac     3720

gagaugcuga uuaagcucua cgaacuguuu uauaaaaagc ugaaagacac aguguacaau     3780

aucagacuuu cuacgcaggu caagacccug gagagcggca aagaaaaguu cgagaagcuc     3840

ucccugcucg auaaaugucu ggugauugaa gagauccuuc aucuguucca gugccagucu     3900

guggccgcua accucagcuc cauugggggc gccgcgacag ccggaauccu gguccugaau     3960

aacgagauua gccgcugugg caagguugaa aucaucaauc aaucuauuac cggguuuuac     4020

gaacagguga ucgaccuuaa aaacauuuga                                      4050


<210>  4182
<211>  4050
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4182
augcagaagu acuaucuggg ccucgacauc gggaccaaca gcgugggaug ggccgucaca       60

gaugagaauu acaaccugug caaauucggc gggaagagaa uguggggcau ucggcuuuuu      120

gacggagcuc aaacggccga agaacgcaga augaaucggu ccaaccgcag acggcugucu      180

cgccagaaac agagaaucga ucuccugcag gaacuuuucg cgaaggagau ugcuaaaguu      240

gaccccaccu uuuucauccg gcugaaugaa agccgccucu auauggagga uaaguccaca      300

gggaccaagu uuccucuguu cgccgacccg ggauacacag auaaagagua uuacaaggaa      360

uaucccacca uuuaccaccu gagacaagag cucaucaaaa acccugaauc ucaugacauc      420

cggcuggugu accuugccug ucaccauauu cugaagaauc gcggccacuu ucucaucaac      480

ggcagcuucu ccgaucugaa aaaucuuucu uuugugugca gagagaugcu ggacgcuuuc      540

aacgagcuca gcaaguauca gcugaugauu uccgauaucg acgcccugga aaagauucuc      600

agcgaucgga aacagcagaa gucugcuaaa gucaaggagc uggugcaaau cuuugaaugu      660

guuccccgcg aggacaaauc cgacgccaag auuugcaaag aaauccuuag caaucugugu      720

aaguuccucg ugggggcgaa gggaaacauc acaagacugu uugauaccga cuucgaugag      780

auggagacgu cuuccuucag cuuuggcgac ucuaaauacg aagauucccu ucugccuagc      840

cucgaggaaa auuaucccga cuacgccaac gucauucaga agaucaaagc uauguaugau      900

ugguccgugc ugguugagau uauggccggg gaaaauuacc ugucuuucgc uaaggugaac      960

gccuauaaaa agcauaagga gaaccucagc auccugaaau cccuuaugaa gaaauacugc     1020

accccugacg ucuaccagcg guuuuucuau gagacagaug aaaagagcuc uuacggaaau     1080

uauauuggcg cgauccacaa caaugggcac ucccauacca ugaaaaagug ugacgaggaa     1140

acauuuuaca agagccuguc uaaaauccuc gauaagcugg uggugcagaa aaccgaccaa     1200

gacacgcuug agauucugaa gaaagaagcc aagaaccaga cacuccugcc gcuggcuaga     1260

accaaggcca auggagucau ccccaaccag auucacaucc aagagcuccg cgcuauucug     1320

gauaaugcca aaacauaucu uagcuuccug aacgaggccg acgaauacgg caccaucgcu     1380

gagaagauca uucggaucgc gaaauuuaag auuccuuauu acgccggccc ccucuccacc     1440

agacauaaag aacagggggc uaauacaugg aucguucgca agcaggaggg acggauuuac     1500

ccuuggaacu ucgaggaaau gguggaucug gagagaucua auaacgaauu uauccgccgg     1560

augacgaaua aaugcaccua ucugauuggc gaggacgugc uucccaagaa cagccuccug     1620

uacuccaagu ucaugguccu gaaugaacuc aacaaccuga aaaucagagg gcacaagauc     1680

agcguggagc uuaaacagga gcuguauaag gaacucuuuu guaaaaaggc caagguuaca     1740

ggaaaagccc ugcuggugua ccuuaagaaa aaggaugcug agcucacccu ggaagacauu     1800

ucuggcuucg augccgacuu uaaauccagc cugucuaucu auauugauuu caagaaucac     1860

auccuccgcg agcaaauuga cgaugggaag uaccagucca ucgucgaaaa caucauuaaa     1920

uggaagacaa ucuucgacgu ggauagcaaa augauugaga agcugaucgc gcgggaguau     1980

ccgaauaugu uuuccucuga acagauuaaa aagaucugca gacuuaagug ucgcggaugg     2040

ggcaacuuca gcucccgguu ucugaauggc auccaggggg agaacaccga aacgaaagag     2100

ugcuucacaa uuaucgaagc ucucuggaag accaauuaca accugaugca acugcuuucu     2160

aaacaguuua ccuucaagga cgccauugac ugcaucaaug ccgauaaagu uggaaagauu     2220

gacaaaguga cauacgagaa caccgucaag gaucucgugg ucagcccugc uaauaagaga     2280

gccaucuggc agacagugca gaucacggag gaaauuaaaa agguuaugaa augugagccc     2340

gaaaagaucu uuguggagau ggcucggggc ggggagaaag aaaagaagcg caccauuucc     2400

agaaaagagc ggcugcugaa gcucuauaug gacuacaaag augaaaacag agacuggauc     2460

aaggagauug aaagccgcga ugagcaagac uucacaaauc ggaaacugua ucuuuacuau     2520

acccagaucg gaagaugcau guacucuggc gaggaaauuu cccuggagga acucaugcag     2580

aagaacagca agugggauaa agaccauauc uauccucagu cuaagaucaa agaugacucc     2640

cuggauaauc ugguccuugc ccgcaaggac auuaacaaca auaaaagcaa caagcuccug     2700

cccacaggga uccaagacaa gaugaaaaag uacuggaaaa ugcugcucaa guacggcuuu     2760

auuucuaaaa agaaguauga uagacugaug cggaccgagg acuucagcga ugaggagcuu     2820

uccggauuua ucgcgcgcca gauuguggag acgagacaga gcucuaaagc cguugcugaa     2880

cugcucgagc accugaauga acauaccgag aucguguacg ucaaggccga acuggugucc     2940

gacuuccggc acgauaacaa uaugcuuaaa agccgcagaa ucaacgacua ucaucacgcu     3000

aaggaugccu uucugaauau ugugguuggc aacgucuaca augcgaaauu cacagccaac     3060

ccccggaauu ggaucaagaa aaaccgcaau accaacuauu ccauuagacg gguguucgac     3120

uacacaguga agcgcggguc uaccaucguc uggcaaggag ugaaggaugg cgggcacagc     3180

auugacggaa ucaaaaagac gauggcuaga aaugauaucc ucuauacaga gcggaccuac     3240

ugugagcagg gcgaacuguu uaacgccaca cucauuaaaa aggggaaccc uaaugcuauc     3300

ggccugaaaa agggacuuga cccgaugaag uacggcgggu auuccucucc caaaaccagc     3360

gccuucgccu uuauugaguu cgacggaaag aaaggcagac agaaccaucu gguugaaauc     3420

ccuguguaca uugcuaauau cgcggcccgc cagcccgagu auauuaagaa ccaccucgag     3480

gagaaaaagg gguaccaaaa ugucaucgug aagaaauauc cuauccagaa gaacgcucug     3540

auuaaacugg auggauaccc cgcccggcuu agaggcgcca augaaaaguc caucacccug     3600

aaaaacgcug uucagcucgu gcugaagccg gaauaucaug agacaauucg caaugucgaa     3660

aaguaccucg aguacaucga caaagaugag ucugugagca aggccgcgcg guuuucccac     3720

acggaacugg acaaacuuua ugaugagcug ugcuucaagg cugaaaacag cauuuacaaa     3780

aagagaccug ccaaucaguc ugccacccuc aaaaacgggc aaaagacauu ucuggcuauc     3840

accaaucuga aggagaaagc ccagguuauc aacaauauua ucacaauguu ccgcugugac     3900

gcgaccacga caaccaaccu uaagaccauu ggcggauccg cuaaugccgg cgggaugcaa     3960

gugaacaaaa acacacugac cagccagaag gucgugcucg ugcaccaguc uaucacagga     4020

cuguuuggca cggaagagga gcuccuguga                                      4050


<210>  4183
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4183
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

guuacagaug acuauaaggu gccugcuaaa aagaugacgg ugcucggcaa uaccgauaaa      120

ucucacauca agaagaaccu gcuuggggcc cuucucuucg acagcggaaa uacagcggcu      180

gauagacggc ugaaacgcac cgccagacgg cgcuacacaa gacggcgcaa ccggauucuu      240

uaucugcagg agaucuuugc cgaagagaug uccaaggucg acgauucuuu cuuucauaga      300

cucgaagaca gcuuccuggu gaccgaggau aaacgcggcg aaagacaccc gauuuuuggg      360

aaucuggagg aggaaguuaa guaccaugag aacuucccca caaucuauca ccuccggcaa      420

uaccuggcug acaauccuga aaaaguggau cuucgccugg ucuaucucgc ccuggcucau      480

aucauuaagu uuagaggaca cuuccuuauc gagggcaagu uugacacccg gaacaaugau      540

gugcagcgcc uguuccagga guuucucgcc guuuacgaca acacguucga aaauuccucu      600

cugcaggagc aaaacgugca ggucgaagag auucugaccg acaaaaucag caaguccgcg      660

aaaaaggaua gagugcucaa acuguuuccc aaugaaaaga gcaacggccg guucgccgag      720

uuccuuaaac ugauuguggg gaaucaggcu gacuuuaaga agcacuucga gcucgaagag      780

aaaacaccuc ugcaguuuuc uaaggauacc uacgaagagg aacuugaggu ccugcucgcc      840

caaaucggag acaacuaugc ugagcuguuc cuguccgcca aaaagcucua cgauagcauu      900

cugcuuucug gcauccugac aguuaccgac guguccacga aagcgccccu cagcgccucu      960

augauccaga gauauaacga acaucagaug gaucuggcuc agcuuaagca auuuauucgc     1020

cagaagcugu ccgacaaaua caaugaggug uucagcgaug ucuccaagga cggguaugcc     1080

ggauacaucg auggcaaaac aaaccaggag gcuuuuuaua aguaccucaa agggcugcug     1140

aauaagauug aaggaucugg cuacuuccuc gacaagaucg aacgggagga cuuucugaga     1200

aaacaacgca ccuucgauaa cggcagcauu ccucaccaga uccaucuuca ggagaugcgg     1260

gccaucauua gacgccaggc cgaauuuuau ccguuccugg cugacaauca agaucggauc     1320

gagaagauuc ucacauuuag aauccccuac uauguggggc cucuggcgcg cggaaaaucc     1380

gacuucgccu ggcugagccg gaagucugcu gauaaaauua cccccuggaa cuucgacgaa     1440

aucguugaua aggaguccag cgccgaggcc uuuaucaaua gaaugaccaa cuacgaccuu     1500

uaucucccua aucagaaggu gcugcccaaa cacucucugc ucuacgaaaa guucacaguc     1560

uauaacgagc ugacgaaagu gaaguacaaa accgaacagg gcaagacagc cuuuuucgau     1620

gcuaauauga aacaggagau uuuugacggg guuuucaagg uguaccgcaa ggucaccaaa     1680

gauaagcuga uggacuuucu ggaaaaagag uucgaugagu uucggaucgu ggaccucaca     1740

ggacuggaca aggaaaacaa agcguucaau gcuagcuaug gcaccuacca cgaucugaga     1800

aagauucuug acaaggauuu ucucgacaac uccaaaaaug agaagauccu ggaagauauu     1860

guccugacgc ucacacuguu cgaggaccgg gaaaugaucc gcaaaagacu ugagaacuau     1920

ucugaucugc ucaccaagga gcaagugaaa aagcuggaac ggagacauua caccgggugg     1980

ggacgccuga gcgccgagcu uauucacggc auccggaaca aggaauccag aaaaacaauc     2040

cucgacuauc ugauugauga cggcaauagc aaccgcaauu uuaugcagcu gaucaacgau     2100

gacgcccucu cuuucaagga ggaaauugcu aaagcccagg uuaucgggga gaccgauaau     2160

cugaaccagg uggucuccga cauugcugga agcccggcca ucaagaaagg cauccuucaa     2220

ucucugaaga uuguggacga gcucguuaag aucauggggc aucagccuga aaauauugug     2280

gucgagaugg cgagagaaaa ccaguucaca aaucagggac ggcgcaacuc ccaacagaga     2340

cugaaaggcc ugacggauag caucaaggag uuuggguccc agauucuuaa agagcacccc     2400

guggaaaauu cucaacucca gaacgaccgg cuguuccugu acuaucucca gaauggccgc     2460

gauauguaca ccggagagga acuggacauc gauuaccuua gccaguauga caucgaucau     2520

auuaucccuc aagccuuuau uaaggacaac uccaucgaua auagagugcu gacaucuagc     2580

aaagagaacc ggggcaaguc cgacgauguu cccagcgaag acgucgugaa ccgcaugaga     2640

cccuucugga auaaacuccu gucuuccggg cugauuagcc agcggaagua caacaaucuu     2700

accaagaaag agcugacacc ugacgauaag gcuggauuua ucaaacgcca gcucguggag     2760

accagacaga ucacgaagca cgucgcccgg augcuggacg aaagauucaa caaagaguuu     2820

gaugacaaua acaagcgcau ucggagagug aagaucguua cccucaaauc uaaucuggug     2880

uccagcuucc gcaaggaauu ugagcuuuau aaaguccggg aaauuaacga uuaccaccau     2940

gcucacgacg ccuaucugaa ugcggugguu gugaaggccc uccuggucaa auacccgaag     3000

cuggagcccg aguucgugua uggcgaauac ccuaaguaca acucuuauag agagcgcaaa     3060

acagcuaccc aaaagauguu uuucuacagc aauaucauga acauguucaa auccaagguu     3120

aaacuugccg augaccagau uguggaacgg cccaugaucg aggucaauga ugaaacaggg     3180

gagauugcuu gggacaagac caagcauauc acgacaguga aaaaggugcu gagcuauccu     3240

caggucaaca ucguuaaaaa gguggaggaa cagaccauug gacaaaaugg cgggcucuuu     3300

gaugacaacc ccaaaucucc gcuggaggug aucccuucca agcucguccc ccugaagaaa     3360

gcccuuaacc cugaaaagua cggcggauau cagaaaccca caaccgccua cccuauucug     3420

cucaucgugg auaccaagca gcugauuccc aucagcguua uggacaaaaa gagauucgag     3480

caaaauccgg ugaaauuucu gaaggacaag ggcuaucagc agaucgagaa aaacaauuuc     3540

gucaaacuuc cuaaauacac acugguggau auugggaacg gaaucaagcg ccucugggcu     3600

uccucuaaag aaguucacaa gggcaaucag cuggugguca gcaagaaauc ccaagaccuc     3660

cuguaccacg cgcaucaccu ugauaacgac uauucuaaug aguacgugaa gaaccauuau     3720

cagcaguuug auauucuguu caaugaaauc acgagcuuuu ccaaaaagug caaacugggg     3780

aaggagcaca uucagaagau cgaagaggcc uacucuaaag agcgggacag cgcuuccauc     3840

gaggaacucg ccgauggauu cauuaagcug cuuggcuuua cccaacuggg ggccacaagc     3900

cccuucucuu uucucggcau caaacugaac cagaagcagu auaccggaaa gaaggacuac     3960

cuccugcccu guauggaagc uacacuuauu caucagucca ucaccggccu guaugagacg     4020

agaauugauc ugagcaagcu cgggggagac uga                                  4053


<210>  4184
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4184
augggcaaga acuacuaucu ggggcucgac augggaacca gcuccguggg cugggccguc       60

acagaugaga aauacagaau ccugcgggcu aaggggaaag accuuugggg aguucgccug      120

uucucugaag ccaagacggc ggcugagaga cggacccacc gcacaagcag acggcgccuc      180

gauagagaaa aagcccggau ugccugccug aaggagaugu uugcugaaga gaucaauaag      240

auugaccccg gcuucuauca gcgccuuaac gauuccaaau acuuucugga ggacaagaaa      300

gaacaucaac cuuaugccau cuucgcugau accggguaca cagacaagga guauuacgau      360

gaauacccga ccauuuuuca ccucagaaaa gcccugcuga auaucgagaa cgacaagaag      420

aaauaugaug ugcggcucgu guaccuggcg auccuuaaua uguucaagca ucgcggacac      480

uuucugaacg ccaaucucga cgauaaaucu ggcggcaacc ugaaggagua uauugaaaug      540

cuucaucaga gcauggucaa uauccugaac auuaaucuca aaaacgugga gaucgaaaag      600

auugagaaaa uccugaaguc cacacaguac ucuaauagcg aaagacugga gaaccuccug      660

gagcuuuaug aacuguccaa gaauaaaaac aagauugaga ccgaaaucuu caaauuuauc      720

ugugggcuca agggaacgau uagcaaaauc uacguugagg acaaguucga agacgaguuu      780

gcuaagauga cccugucuuu ccgggauucc aacuuugacg agaaaauuau cgaaauugag      840

gauaaucuug acgaugacaa guaugauaug uuccugcuca ucaaacagau ccacgacugg      900

agcgugcugg ccaacauuau gaauggcgaa gaguaccugu cugucgcucg cgugaagcuc      960

uacgauaaac acaagaagga ccuggaaguu cuuaaaaagu auuacaaaca aaacuccaug     1020

gaggaguaua auaagauguu cagacagaug aacgauggga auuacagcgc cuauguggga     1080

ucugucaucu acaaagacaa cuccgugcgg agaggcugca agagcaagaa agaagacuuu     1140

uauaagaaua uucugaacac aaucaaaucc ugggaggauu gugaagcgaa ggaguacauu     1200

accucugaga ucgacaaagg gaauuuccuc cccaagcaga ucacagccag caacggagug     1260

auuccuaauc agguccauaa aaaggaacug aagaaaaucc uuaagaacgc uuccgaguac     1320

cugaauuuuc ucaacgaaaa agaugagagc ggcuauacca uuucugaaaa gaucguuaaa     1380

cuguucgagu uucaaauucc cuacuaugug ggcccuaucg ccuacaacau ugggaaugac     1440

gaguccaagc accgccauaa cauguggagc gugcggaagg aaaaaggacc caucuauccu     1500

uggaauuucg agcagaagau cgauauuaaa aagucuagcg aaaaauuuau cagaaaccug     1560

auuaaucacu gcacguaccu caacgacgag gaaguccugc cgaaaaauuc ccuucuguau     1620

gagaaguuca uggugcucaa cgagcugaau aaacugaaga ucaacggcga aaagauuucu     1680

guugagcuua agcagaauau cuuuaacgau cucuucaaaa aggggaagaa agugacaaag     1740

aaaggacuga ucaaguaccu gaaagaacag ggcgagauug acaauuguga agaggucgag     1800

aucagcggga uugauggaga cuuuaccaac aagcucucca auuacaaaaa guucgcugau     1860

aucuuuggcg ugcaaagccu gacauaugaa cagaccgaca uugccgagaa caucauccgc     1920

uacucuacca uuuauggcga uucccggaag uuccuugaag agagaauccg cgaggaauac     1980

agcaacguuc uggacgagaa acagauuaag cggauccucg ggaugaaauu caaggacugg     2040

ggaagacugu cuaaagaacu gcuugagcuc uccggcgugg auaaggaaac aggggagauu     2100

gccagcguca ucucccgcau guggaaugac aacuauaauc ugauggagcu gaucgcuacg     2160

gaacgguuuu cuuacgcgua ugagauugaa caaagaagca agaaacucga gaagaccauc     2220

uccacaauug aauacgagga ucuggacgag cuuuacaugu cuacccccgu gaaacgcaug     2280

gucuggcaga caauccuggu gcucaaggaa cuggaggaag uucugggaug cccucccaaa     2340

cggauuuucg uggagauggc cagagauguc aacgcuccua aggaccggaa ggauagccgc     2400

aaaaagaaac uucucgaacu guauaagaau uguaaaaagg aggagggcga cugguccaag     2460

gaacugaacg ccagagagga aagcgaguuu aaaucuaaga aacucuaccu guauuacaug     2520

cagaaggggc ggugcaugua uuccggcaaa cccaucgauc uuaagaaucu gcucaacgac     2580

aaucuguacg auauugacca caucuauccg agacauuucg ugaaggauga cagcaucgag     2640

aacaaucugg uucuugugga aaagaacuuu aaugcccaca agucugacaa cuacccuauu     2700

gagaaugaaa uccgccagaa aaugcauucc auguggaaga ugcuccggga uggaaacuuc     2760

auuaccgagg aaaaauacag cagacugauc cgcaaggagg aguuuacgga cgaugaaaaa     2820

gcuaaguuca uuucuagaca aaucgucgag acacggcagg gcaccaaggu gaucaccgac     2880

cuguuugaac agacauuccc cgagagcgau auuguguaug uuaaagccgg gaacgucucc     2940

gaauuucgcc acaaguucaa acucaucaag uguagaaaug ugaacgacuu ucaucacgcg     3000

aaugaugcuu accugaacau uguggucgga aauguguauu acaccaaauu cacaaagagc     3060

gcccuuaacu ucaucaagga cuauaaaaag aauccugaga aaaacaagua ccacauggau     3120

cauauguuuc aguaucccgu uucucggggc gggguggacg ccuggauuac gaaaggagcu     3180

gaguccauca gcaugguccg caagaugaug gccaagaaua cccccauggu gacaagaaug     3240

aacuacgaaa aacacuccgg ccaaaaaggg ggcaucucug aucagaauau uugcagcgcu     3300

aaaaucgcca agaaaaacgu uuaccugccu auuaagaugg gagacgagaa gcucaaaaau     3360

guggaaaagu auggcgggua cggaucccug accgcgaaau auuucuuucu ggucgagcau     3420

acaaucaaga aaaagcggau ucgcaccauc gaagccuacc cgauguaucu ugacgcucug     3480

aacaaagaga auaagcuccu gaacuacugu gugcagguuc ucgaguauga ugaacccaag     3540

auuaucaugg agaaaaucaa ggugaaaucu cuguucaaga uuaauggcua cuacuauuac     3600

cuuaacgcca aaagcgucaa cagaaucauu ugggggaaug cuacgcagcu guuucucgaa     3660

aacgacauca augaguaugu gcggaagauu uccaagaaca ucgagugcau cgauauuaau     3720

ucugaaaaca auaucaaagu guacgacauu uucaucgaga aguauagcaa caaacuguuu     3780

uccaaucgcc ugggaaaccu uaaggaagcc auuauccaaa auagaaacaa auucaucgag     3840

cugaagauua augaacagau caacgucauu cucgagcugg uucgguacag ccaggauggc     3900

guguauucua ucaaucucuc ccagcugggg cuuagcucua ccuacgcgau uaacagaaca     3960

uccaaggaga ucagcaaaau gaaggaaugc aaacugauua accaaucugu gaccggccuc     4020

uacgagaaug aaaucgaccu gcugacaguc uga                                  4053


<210>  4185
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4185
augcagcaau acuaucuggg cguggacaug gggagcgccu ccgucggaug ggcuguuacc       60

gaugagaagu accagcucgu gcggaaaaag ggcaaagacc uguggggagu gagaacauuc      120

gauaucgccc agacggcgga aguccgcaga gugucucgga ccaaccgcag acggcagaau      180

cgcagaaagc aacggauuca gauccuucag gagcugcucg gggaagaggu ucugaaaauu      240

gacgcuggcu uuuuccaccg caugaaggaa agcagauaug uggccgagga uaagcggaca      300

cuugacggga aacaggucga gcugcccuac gcccucuuug uggaucaagg auucaccgac      360

aaggauuuuu auaaacaguu cccuacaauc aaccaucuga uuguuuaccu gaugaccaca      420

uccgacaccc cggauauccg ccucguguau cuggcccuuc acuacuacau gaagaauaga      480

ggcaacuuuc ugcauucugg cgacaucaau gaugucaaag acauucagag cauccucgaa      540

cagcuggaga acgugcuuaa ggaauaugug gacgauuggg agcugucccu caaggacaaa      600

gucgaugcua uuaaggagau cuacaauaaa gaccuggggc ggggagaacg caagaaggcu      660

uucauuaaca cgcugggcgu uaagaccaaa ucugccaagg cguuuugcag ccucaucucc      720

gggggaagca caaaucuggc cgagcuuuuc gaugacucug gccugaagga auccgaguau      780

gcuaaaauug aauuugccaa cgcuaauuuc gaggauagcg uggaggggau ccaagcccuc      840

cuggaagaca gauuugcggu gaucgaggcc gcuaagcggc uuuacgauug gaaaauucug      900

accgacaucc ucggagauaa cgccucucug gcugaagcca gagucaaguc cuaugagaca      960

caccaugaac agcuggugga gcucaagagc uucauuaaga aguaccugga ccgcaaaauc     1020

uaucaggaca uuuucaucaa ucccaacauc gccaauaacu acccugcuua cguuggccac     1080

accaagauua acggcaaaaa gcaagagcuu gaagugaaac gggcgaagag aaaugauuuu     1140

uaugccuaca ucaagaaaca ggucauugac cccaucaaga aaaagguguc ugauaaagcu     1200

guucuggccc gccucgccga gauugaaucc cugaucgagg ugaacaagua ucuuccucug     1260

caggucaaua gcgacaacgg ggugaucccc uaccagauua agcucaauga acugcggaga     1320

aucuucaaca aucuggagaa ccgccucccu guccugaaag agaaucggga uaagauuauc     1380

aaaacguuuu ccuaucgcau uccguacuau gugggacccc uuaacggcgu uaauagaaac     1440

gggaagucua caaauuggau ggugcggaaa gaaggagagg aaggcaagau cuacccuugg     1500

aacuucgagg agaaagucga ccuggaagcu agcgccgaga aguuuauuag acgcaugacc     1560

aauaagugua cauaccucgu gaacgaagau guucugccca aauauucccu gcuuuacagc     1620

aaguaucucg ugcugucuga gcugaacaau cuccggcugg acgggagacc ucuugaaguc     1680

uccgugaaac aagagaucua cgagaacgug uucaagcgga aucgcaaagu uacccugaag     1740

aagaucaaaa acuaucuccu gaaggaagga gucauuagcg agaaagauga acugucuggc     1800

cuugcggacg augugaaaag cucccucacc gcuuaccacg acuuuaaaga gaagcugggc     1860

caucugacac ucacggaaga ucagauggag aagaucauuc ugaaugugac ccuuuucggg     1920

gacgacaaaa agcugcucaa aaagagacug gccgcccugu aucccaacau cgaugagaaa     1980

ucucuuagcc ggauggcuac auuuaauuac agagacuggg gacgccucuc caagaaguuc     2040

cugagcgaaa uuaccucugu cgaucaggag acaggcgaac ugcggaccau cauccagugc     2100

auguacgaga cgcaaaacaa ucucaugcag cugcuuuccg aaccguauca cuuuguggag     2160

gccauugaga aagaaaaccc uaagguugac cuggagagca ucucuuacag aauugugaau     2220

gaucucuaug ucucccccgc ugugaaacgc cagaucuggc agacacugcu gguuauuaag     2280

gacaucaaac aagugaugaa gcaugauccu aaaagaaucu ucauugaaau ggcccgggag     2340

aagcaggaga gcaagaccac caaaucccgc aagcaggucc uuucugaagu guacaaaaac     2400

gcggagaagu auaaaaaucu cuuugaaaag cugaacagcc ugacagagga acagcucaga     2460

uccaagaaag uuuaccugua uuucacccaa cuugggaagu guauguacac aaaugacgcc     2520

aucgauuucg agaaccuggu gucugcuaac agcaauuacg acauugauca caucuauccc     2580

caguccaaaa cgauugacga uagcuuuaac aaucucgucc uggugaagaa aggaaucaac     2640

aaugacaagu cugaccggua ccccaucgau aagaacauuc gcgacgauga gaaagugaag     2700

acccugugga auacacuucu guccaaaggc cucaucacca aggaaaaauu cgagagacug     2760

auucggagca caccuuuuuc ugacgaggaa cucgccgggu ucaucgcucg ccagcugguc     2820

gaaaccagac aauccacgaa ggccguugcg gagauucuua gcaacugguu uccggagucu     2880

gaaaucgugu auagcaaggc caaacacauu accaauuucc ggcaggauuu ugagauccug     2940

aaggugcgcg aacucaacga cugccaucac gcucaugaug ccuaccugaa uaucgucgug     3000

ggcaacgcuu aucacacaaa auucaccaau ucccccuaca gauuuauuca gaacaaggcc     3060

aaucaggagu auaaccugcg gaaacuucug caaaaggcca agaaaaucga aagcaacgga     3120

guuauugcuu ggaucggcca gucugagaau aacccuggga caauugcgac cgugaagaaa     3180

gucaucucca gaaauacggu gcucaucagc cgcaugguua aggaggugga cggacagcug     3240

uucgaucagc aacucaugaa aaagggcaaa gggcaggucc ccauuaaguc cucugacgau     3300

cggcugaucg acauuagcaa guacggaggc uacaacaaag ccaagggggc uuauuuugug     3360

uucaucaaau ccguuagacg cggcaagaca auuaaaucuu uugaauacau cccugugcau     3420

cuugccaaga aguucgacug uaaucuggag cuccugaaag aauaucugga gagcgagaag     3480

gaucuuaaca augucgaaau ccugaugccc aaagugauga uuaacucccu cuucaauuac     3540

aacggaucuc ugauccggau uccgggcaga uaugacaaga aaagccuccu gaucaaugug     3600

gaugucccuc uucugcugga gucccagcac auuaagcagc ucaagguuau cgaaaaauac     3660

auguauaaga aacgcgugag caagaacucu aauauucugc uuaccaaauu ugccuccgac     3720

caacugaagg aucucgacgc ucuguucgau gugcucagcu acaagcugaa cgagaauauc     3780

uacaacguca ucaaugacaa auaugauaaa cuugugauuu gccgggacaa auuuaucucu     3840

cuggauacag aaguuaagug ugagaugauu uucgagcugc uccaccuguu ucagugcaac     3900

ucccagcuug ccaacaucac caaaauuggg gcgaccagca aguucggauc uaucuccaug     3960

agcaaaaauc ugaaggaaaa cgacaagaug uccaucauuc aucaaagccc cucuggcauc     4020

uuugagcacg aaauugagcu cacagcucug uga                                  4053


<210>  4186
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4186
augaagagaa uccugggccu cgaccugggg accgccagca uuggaugggc ucuugugaac       60

gaguccgaaa augagaaaga aaagucugag aucauuaaac ugggcguccg gguuaacccc      120

cucacaacgg augaacagac cgacuucgag aaggggcgcc cucugagcgu gaaugccgau      180

agaacacuua aacggggagc gcgcagaaac cugcaacggu uuaagcagcg cagaaagaau      240

cucaucgaga uucugaucaa aaagggcuuc aucaccaaca uuucccugcu cacagaaauc      300

gggucuaaua ccacauacca gacccuggag cuucgggcua aaagcgugaa ggaaaagauu      360

gagaaggagg aauuugcccg cguccugcuc acgaucaaca agaaaagagg auauaagucc      420

ucucggaaag ccaagaauga cgaggaaggc agccugauug auggcauguc cguggcuaaa      480

gagcuuuacg acaacaaucu gaccguuggg caguaugugu accaacuccu ggaaaaggga      540

ggcaaauaua ucccggauuu cuaccgcagc gaccugcaga acgaguuuga ugucauuugg      600

aauuuccaga aggaguuuua ucccaacauc cucacagggg aacuguacaa gaaacuucag      660

ggacaaggcc agcaaaauuc uagaaagcug uuccucgcca uccaccagau uuacaccgcu      720

gagaacaaag ggaagcggga cacagugaaa aagcagcauu auaaguggcg caacgaugcc      780

aucaauaccc agcugcuuau ugaagagguu gcguacgugc uggucgaaau caacaauaac      840

cucaauaaau ccagcggaua ucugggcgcc auuucugaca gauccaagga gcuguacuuu      900

aacaaagaga cggugggcga aaaucucuau aagcagaucc aagagaaccc ucagacaagc      960

cugaaaaauc agguguucua ccggcaggau uaucuugacg aauuugagca aaucugggaa     1020

acccaggcua aguucuacuc ugagcugaca aaggagcuca aagaagaggu cagagacguu     1080

gugaucuuuu accagcgcaa gcugaaaucc cagaagcacc uuauuagcga augcgaguuc     1140

gagaaacauc acaaggccau ucccaagucc ucuccucugu uccaagaauu uaaaaucugg     1200

cagauucuca acaauaucga guucaagcau aaaaagacca aagaacugaa gauccugagc     1260

gaggaacuca aagagacccu guuugaggaa cuuaacauuc gggggaauuu cacauccgau     1320

gcugugcuga aguuugucaa gcucgacaaa aaggaguggg aacugaacua uaaaaaggga     1380

aucgagggca auagaacgaa cgaagugcug uacaacguuu aucagaaaau ugccgaguac     1440

gagggguaug gauucgauug gagcaagaag ucugcggugg aaaucaaaga ggaacuuaau     1500

accaucuuuu ccgagauugg cauuaacagc aagauccucg acuucaaucc cgaaaucucu     1560

gggaacgauu uugacaaaca ggagagcuac caacuguggc aucugcucua uuccgccgag     1620

gaagauggau uccugaagca gaaacuucag gacaaguacg gcuuuaaguc ugaguacgcu     1680

aauaugcugg ccaacgucag cuuccagucc gauuauggca gccucucugc ucgcgccauu     1740

aaaaagaucc ugccuaaucu gauugccggg cacgaauuuu ccgaggcuug uagccuuucu     1800

ggauacaacc acuccaauag ccucacaaaa gaggaacugg cgaaccggcc gcugaagggc     1860

aaacucgagc ugcuuaagaa aaauucccug agaaaccccg ugguugaaaa gauccucaau     1920

caaaugguga accuggucaa ucaggugauu gagucuuaug gggaaaucga cgagguccgc     1980

aucgagcugg cccgggaacu uaagaaaagc gcucaggaga gagcccucau gaccacaaac     2040

auuaacgccu ccaagaccaa auacgaaucu uuccgcaagc ugcugcaaaa agauuucgga     2100

aucaagaauc cuacgcggaa cgacauuauc aaguauagac ucuacgagga acugaaagcu     2160

aauaaguauc acacaauuua caccaacaaa uacauccccc gcgacaaacu uuuuagcaaa     2220

gagaucgaua uugagcauau cauuccuaag gccaagcugu ucgacgauuc cuuuagcaau     2280

aaaacccucg cguuccggaa gcccaaccag gacaaaggca auaugacagc uaucgauuau     2340

auucugucuu accaguccaa ggacaucgau aacuauaaaa gcagaaucga aaaucuguuu     2400

aagaacggga agggcgacau uucuaaagcc aagcggaaua aacuucucau ggaggaaaag     2460

gauaucccgg acggauucau ugaucgcgac cugagaaacu cccaguacau cgccaaaaag     2520

gcuaagcaac ugcucgagga agugauucgg accguuaaua caacgaccgg cagcgugaca     2580

aacaaacuga gagaugacug ggggcuuauc aaugucaugc aggagcugaa caugccuaag     2640

uuuaaagcca uugacgaguc ucucaucgaa cagaucgaga agaaagaugg aaccuucaag     2700

acacgcauua aggacuggag caaacggaac gaucacagac accaugcuau ggacgcccug     2760

accguggcgu uuacgcgcua uaaucacauc caguaccuga acaaucuuaa cgccaagucc     2820

gauaaaaagg gcgcuauuua ugggauccaa gaacuggaga ccuacguuaa uagcaaagga     2880

aagagaaaau ucauuaagcc caugcauaac uuucgggagg aagccaagaa acacaucgag     2940

gacauccuca uuucuuucaa ggcuaaaaau aaggugguca caaagaacau caauaagauu     3000

aaguccaaag auggcuuuau caagaaagug cagcugaccc cucgcgggca gcuccauaag     3060

gaaacagugu acggcaaaau uaacgagguu gucgugaagg aagagaagau caaugccaaa     3120

uucgaccugg agaagaucaa caaagugacc aagcccauuu auagaaaugc gcuucugaaa     3180

cggcuccagg aguucaacgg agaacccaag aaggccuuua cgggcaaaaa uagccugucc     3240

aagaacccua ucuaccugga ugucgcucaa acaaaaaccc uuccggaauc ugugaagguu     3300

caguucuuug aggaaaauua uacaauucgc aaagagauca gccccgaccu gaagauugau     3360

aaaaucauug acaaggggau ccagagaauc cuccugaagc ggcucgauga guucaacgga     3420

aaaccuaagg aagccuuuuc caaccuggag aaaaauccca uuuggcuuaa caaggaaaaa     3480

ggcaucgcua uuaagcgcgu gaagaucaaa ggggucaaga auguggaguc ucugcacaac     3540

aaaagagacc accucggaaa ugaaauucug caaaagggca aaagcgcccc uaacgacuac     3600

guuuccaccg ggaauaacca ucacguggcc aucuaucagg auaagaaugg caagcugcag     3660

gagaaagucg ugucuuucua cgaggcuguu gcgcggguga acaauaaacu ucccaucauu     3720

gaccaggauu uuaacaaaga ccugggaugg aaguuccucu uuaccaugaa acaaaaugaa     3780

auguucgucu uuccgagcga ggauuucaag ccucuggaaa cagaccucau ggauaagaaa     3840

aacaauucca agaucagcaa aaaccuguuc cgcgugcaga aacuuggcaa caaggacuau     3900

acguuuagac aucaccugga gaccaagauu gaggauauca aaaagcugcg ggacuucaca     3960

uacauuagaa agcgcaaucc cgaaucuauc aaaaacauca uuaaggugcg gaucaaucac     4020

cucgggaaca uuguccaugu uggagaguac uga                                  4053


<210>  4187
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4187
augagcaaga aauacuaucu gggccucgac aucgggacca acuccguggg augggccguc       60

acagaugaga auuacaaccu gugcaaguau gcuggcaaaa gaaugugggg gauucggcuu      120

uucgaaggag gcgagacggc cgaacagcgc agaaucucuc ggagcaaucg cagacggcug      180

ucccgcaaga aacaaagaau ugaccuccug caggagcuuu uugcggaaga gaucgcuaag      240

auggauccca ccuucuuuau ucggcugaac gagucucgcc uccaccugga agacaagagc      300

acaggggaga aauucccucu guuuagagau aaggacuaca ccgauaucca guauuacaaa      360

gaauauccga caauguacca ucuccggaag gagcugaucg aaaauuccaa aguucacgac      420

auucgccuug uguaccuggc cugucaucac auccucaaga acagaggaca uuuccugauu      480

aauggcucuu uuagcgaugu gaagaaacuu uccagcgucu gcgaaucuau gcugaacucc      540

uucaauggca ucagcaagua ucagauugag aucaacgaca uugaacggcu cgaggaaauc      600

cugucugauc gcaaaaagca aaaauccgug aaggccaaag agcugaucca gauuuuugag      660

ugugggcccu gcgaagacaa gaccgaugag aagacaugua aagcuaucgu uagcaaucuc      720

ugcaaguggc ugaccggacu uaaaggcaac auuaauucua ucuucgacau gcaguuugaa      780

gagauugaca cguccagcuu cuccuuuacc gaaccuaagu acgaggauac aaucucugcc      840

gcucuggagg aaaaauaucc cgacuacgcc gaugugaucc agaagauuaa ggcggucuau      900

gacuggagcg ugcucguuga gaucauggcc ggggaagauu accuguccuu cgcuaaagug      960

aacgccuaua auaagcacaa agagaaccuu uguauucuga aggaagucau cagaaaauac     1020

ugcgacagcg agauuuacca caaguucuuu aacggauucg augagaagaa uggcuauggg     1080

aacuacaucg gaucuaucua uaaaaauggc cauuccuacg cuaugaagaa auguagcacc     1140

gaagacuuuu auaagacacu cggcaaaauu cuggauaaga uccaaccuga gaaggaagac     1200

gagaacgugc uggaggaacu caaaguggag gucaagaauc agacccugcu uccccuggcc     1260

cggacgacag cgaacgggac cauuccuaau cagguucaca uccaggaacu cagagccauu     1320

cuggauaacg cuaaaaagua ccuuccguuc cugaaugagg aagacgagua uggaacagug     1380

gccgagaaaa ucauuucuau cgcuaaguuu cgcauccccu acuacguggg cccucucagc     1440

acccggcaua aagaacaagg ggccaacucc uggauuguca gaaagcagga gggacgcauc     1500

uaucccugga auuucgaaga gaugguggac cuggaacagu cuaacaagga guuuauucaa     1560

cggaugacca auaaaugcac auaccugauc ggcgaggaug uucucccuaa gaacagccug     1620

cuuuauucca gauacauggu gcugaaugaa cucaacaacc ugcgcauucg ggggaauaaa     1680

aucagcguca agcugaaaca ggagcuuuau aaggaacucu ucuguacgaa ggccaaagug     1740

accggaaagg gccuguuuaa cuaccugaaa aaggaggacg aggagcucgc ucuggaggau     1800

aucucuggcu ucgacauuga uuuuaaaucc agccuuacau cuuaucugga cuucaagaag     1860

cagauccucg gggaagagau ugaaaaagau aaguacaaag gaaucguuga gaauauuauc     1920

aaguggaaaa ccaucuacga cgaugacucc aagaugauga agaaaaugau ugagagagaa     1980

uaucccaacg uguuuagcaa ggauaaaauc aagaaaauuu gccgcuucaa guacuccggc     2040

ugggggaauu ucucucugag cuuucugaac ggaauccggg gcgcggacag agagacaggg     2100

gaauccuuca ccauuaucga ggcccuuugg aaaacgaauu auaaccucau gcagcugcug     2160

ucuaagcaau uuacauuccg cgaagagauc gaucucauua augcugagaa ggucggcaaa     2220

aucaacaagg ugagcuacga caauaccguc aaagaccuga uugugucccc ggccaacaag     2280

cgggccaucu ggcagaccgu ucagauuaca gaagagauca aaaaggugau gaagugcgaa     2340

ccugagagaa uuuuuaucga aauggcucgg ggaggcgaga aagagaagaa acgcaccguc     2400

agcagaaagg cccggcuucu ggaacucuau gcggcuugua aagaugacgu gagagauugg     2460

acaaaggaga ucgaagaccg cgaggaacgg gaguucaauu cuaagaaacu guaccuguau     2520

uacacgcaga uggggagaug cauguacucc ggagaggaaa uuaacaucga ugagcuuaug     2580

caaaagaaua gcaaauggga cauugaucac aucuaucccc agucuaagau uaaagacgau     2640

ucccucgaca acaugguucu ggccaagaag gugaucaaca auagcaaaca gaacaagcug     2700

cucucuagcg auauccgcau uaaaaugaag aaauacugga augcccugua uaagggcaac     2760

uuuaucucca agaaaaagua cgacagacuu acccggacag aagauuucac caaugaggag     2820

cugagcgggu uuauugaacg ccagaucguc gagacaagac aaucuuccaa agcuguggcc     2880

gaacuccuga accggcugua ugagcacagc gaaauugugu acguuaaggc uggacuuguc     2940

uccgacuucc gccaugacaa uaaacuccug aagucuagac ggaucaacga uuaucaccau     3000

gccaaagacg cguuucugaa uaucguggug ggcaacgucu acaaugccaa guucaccagc     3060

aacccucgca aguggauuaa agagaauaga gggacgaacu acuccauccg gcgcguguuu     3120

gauuauaaug uuaagagagg caacaccgug gucuggcagg gaguggagga cggcgggcac     3180

ucuauugaug aaauccggaa aacaauggcu agaaacgaca uucucuacac cgaguauaca     3240

uacugugaua agggagaacu guucaaugcc acccuuauca aaaagggcaa cgacaaggcu     3300

auuaaucuga aaaaggcccu cgauagcguu aaauaugggg gauacaacuc ccccaagacg     3360

ucugcguucg ccuuuaucga guucgacggc aaaaagaagg aacgcaaaaa ucauaucgug     3420

gagauucccg ucuauaucgu gaacauugcu aagcggcagc cugauacaau caaagaguac     3480

cuggaacaga agaaagggua caagaauguu guggucaaga aauauccgau uaagaaaaac     3540

gcccugauca agaucgacgg cuaccuugcu agacugcgcg gagccaauga gaccgacauu     3600

cuccugaaaa acgccgugca acucauccug aaugauaagu auuacgaaau uauccggaga     3660

guggagaaau aucuugaaua caacguccgc uaugaggcug agagcauguu ugaaggcuuc     3720

gaccacgcgg aucucaauuc ccuguacgac gagcugacaa gcaagcugga aaccucuauu     3780

uacaagaaac ggcccgccaa ccagcuuaau gcucuggaga aggggaaaga gaaguuuaaa     3840

aacauccaga cccucagaga aaaggcccaa aucauuaagg agaugcugau cauguaucgc     3900

ugcgaugccg cuacaguugc caaucucaaa gcgauuggag gcuccgcuaa cgccgggagc     3960

augaagauca aaaagaacac gcugaccucu cggucccuug ugcuggugaa ucagagcauu     4020

acaggacucu ucgaaaccga ggaggaacug gucuga                               4056


<210>  4188
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4188
auguacaaga aauauggcau cgggcuggac auuggaacca gcuccaucgg cuggucugug       60

gucgaugagu acgggcacau uaucagaguu aagggacaga caggccucgg ggugcggcug      120

uuccgcgaag gacaacccgc cgcugacaga cggacguuuc gcaccacaag acggcgccuu      180

ggcagacggc gcuggagacu gcggcuccug cgcgagauuu ucgauccuua uaucgccgcg      240

caggacccga acuuuuucgc uagacagaaa gccagcgcca ucgcucccgg cgauucccgg      300

uuuaaggccg uguaccgccu uuucaaugac cagaccgaua gacaauuuua ugacaaauac      360

ccuacagucu aucaucugcg gcagaagcuc augaccgaaa agagacaguu cgauauucgc      420

gagaucuacc uggcugugca ccauauuguu aaauaccggg ggcacuuucu gacaggaggc      480

gccgcgaaga acuucaaacc cgggaagcuc gaccugaaua ccuauuuugc cacgaucaac      540

gcucagcuug ccgcuauuuu cauggauucu caaguggaac ugccucagcu cgacugggau      600

gccgucguga gccugcuuac caaugaccag gagucccccu cugacagaca gaaagcgcug      660

acaaagaugc ucguugccca aggagaugcu acccagaagc gcuuuuacac agaguucagc      720

aaagcccugc ugggccucaa ggcuaaacug uaugugcuua ccggguucga aggagacaag      780

acggaucaga aagcccugac acucucccug gagacccuug acgaucauca agacgauguc      840

acacagcugc ucggcgacga acagcggcag cugguggagc ugcucgccag agugcaaagc      900

gcuauccagc uggcgauuau caucccuaac ggcaagaccu uuucugggca gaugguccag      960

caauaccaga cccacuauga ucagcuucgc cugcucaaag acuaccggga gcagcugaca     1020

gaucaaaagc acagacgcgc ccuuaagcag gcuuaugccg acuaccuggc caaugaccag     1080

gaucaggacg cucucguuaa agccguggaa aaggugcuga aacaaaacca gccgcugucc     1140

acggucggag ugcggcucca gaccgcgauu gaugcuggcg acuuccugcc caagcaaaga     1200

acaaaagcca auagcguuau cccucaucag auucaccagc aggagcuuga ucgcaucauu     1260

gaagcccaau cuguguacua ucccuggcug gcugaggaaa acccugucgc cgcucaucgg     1320

uccuacgccc ccuauaagcu cgacgagcug guggauuuua gaguuccgua cuaugugggg     1380

ccucuggucc agccccaggc ggacaaggcc gcuaaagaga ccaaguucgc cuggaugaca     1440

cggaaagaag cucagagcac cacgccuauc acacccugga auuuugagca agaaguggau     1500

cgcaacgccu cugcgaaugc cuucauccag agaaugaaga ccaccgacac auaccuucuc     1560

ggagaggaug uccugcccaa aaacucccug cucuaucagc gguuugaagu gcugaaugag     1620

cuuaacaacg uuagagugga cggcaagcug cucaccauug cugagaagca gcgccuguuc     1680

caagaucugu uugucgaccg gagccaggcc uccgugacaa ucgcucagcu ucaagccaau     1740

cucauugccc acggggcugc gaaagacaga guugaaauca agggacuggc cgauccuaaa     1800

cgcuuccugu cuagccucac gaccuaccac gacuacgcuc agauuauccc ggaggcccug     1860

gaacagcccg auaagagagc cgacaucgag aaaauuauca acugguccac aauuuuugaa     1920

gauaccagca ucuucaagga gaagcugcag gagauugcuu ggcugacacc ugaccaacgg     1980

cgccagcucu cucagaaaag auaucggggc uggggccagc uguccgauaa gcugcuuacc     2040

caacuccgcg acgccacggg gcagagcgug auggaucugc uguggaccga acccaauaac     2100

uuuaugcaga ucauuagacg gacagaguuc cagcaagcga ucgcucgcgu caaucagacc     2160

gaccucaaac agcaggaucu ggccacaacc aucaacgacc uuuacacguc uccucaaaau     2220

aagaaagcca uuagacaggu gcugcucgug guugcugaua uccagacagc caugcaugaa     2280

gucgcgccca gcuggcuguu cauugaggcu gcccggggac cggagcaaaa cccuaagcgc     2340

aaacuggcca gacagcagca gcuucucgag gcuuauaaga ccuccgccaa ggagaucgug     2400

aaugaagcug ugaaagagaa gcugcugucu gccauugacg accaccaaaa auuugaagau     2460

caggucgugc ucuacuucca gcagaacggc cuggacaugu auacagggaa gccccuugau     2520

uuugagcggc ugagcaaaua ccauaucgac cacaucauuc cucaauccgu ugugaaggau     2580

gacagccucg auaauagagu ccugaccacc cccgaggaaa acgagcgcaa gggagcgcgg     2640

gugccuggcc cccuguaugc cucucagcag gggagauggc gccuucugca gcgggcuggc     2700

cucaucuccc aaagaaaacu ggaccaucuc cugcuucgcc cggaagaacu ggaucagcag     2760

gccacaggau ucguugcucg gcaacucgug gaaacgagac aggucauuaa gcugaucacc     2820

aauauucuga gccagcagua cgacccugag caaacaaaac uugugucugu uaaggccggc     2880

cugucccacc aguuucgcac ccggcucaac cugcccaaac ucagagagcu gaaugauuac     2940

caccaugccc acgacgcuua ucuugcggcc cggaucggga cauaccugcu ccagcgcuau     3000

cccaaccugg aaagauucuu uguguacgga caguaucaaa agacccgguu cgaccugaag     3060

cgccauuuua acuucaucgg cgagaugcuu aaagggaagc cugaagaugc ugucauugug     3120

aaagccccgc acagcgagac ggugaucugg gacaagcagg augaagucaa acagcugaag     3180

aagauuaugg aguacagaca ccuccugguu acacaugagg uguaugacaa ucacggagcc     3240

cucuuuaacc agaccaucca ucccgcuaaa aauccuaaga accggaaacu gauucccaga     3300

aagaccacac aauccaccga acuuuacggc ggguacucug gcgagacaac gggauaucug     3360

gccaucgcgc gcaccgugac aaagaauaag aucagcuacc agguccugcg ggugccuacc     3420

agacucguug gccgccugca gcgguccucu gaaagcgcuc agagacaaau ucuuacagau     3480

ugguucgccc ccuccuuugu gagccgcggg aaacagcagc cuuucgagau cguccugcgg     3540

cacguguuca agaaccaaac cguuauugac gauauccaug agcagucuca cagauuugga     3600

cuccgcuccg acacguauua cugcaaucag cagcaacugg ugcucagccu ggaacagcag     3660

agaaugauua ucgaucagaa acuuaacaau accaacaaac ugauggucgu guaugaggcc     3720

guuugugacc aaguggcuaa guacuucccc cuguaucgga ugaaucacuu uaaagaaucu     3780

cuccuggagg ccggcccgaa acuugcggaa cugccuauua acacagucca guccaagaau     3840

gggaagcuca agcugggaca gcgcagcgug cuccugcaga ccaugcaagg ccuucaugcu     3900

aaccccgaga caaaagaucu gucuaucauc gggugcccua aguacuucgg caaacuucag     3960

gugagcuccg gaauuaagcu caccgagaau gccguccuga uccacgaaag ccccacaggc     4020

cuuuuucaga gacggguucg ccugucugac cucuga                               4056


<210>  4189
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4189
augaagaucc ugggccucga ccuggggacc aacagcauug gaugggccgu ggucaaugag       60

gcugaaaacg agagagaauc cucuagcauc auucggcuug gcguucgcgu gaauccccug      120

acaguggaug agcagaacaa uuucgaaaaa gggaagccua ucacgaccaa cgccgacaga      180

acacucaaac gguccaugcg cagaaaucug caacgguaca aacuucgcag agcgaaccug      240

gucucucucc ugaagaccca gggauggauu acagaugaga cccugcucag cgagcagggc      300

aauaacucca cauuugaaac cuaucggcug cgcgcugagg ccgccacgaa ggaaaucacc      360

cuugagcagu ucgcuagagu gcugcucaug cugaauaaga aacgggggua caagucuagc      420

cgcaaaauga agcacgacga ggauggaucc uuuguuaaca aucuugacau cgccagagaa      480

cuguaugaua gcggccucac accgggccaa uacgcuuaua accugcugaa agacgggaag      540

aaguacauuc ccgauuucua ucggucugac cuccaggagg aacuggauaa aaucugggcc      600

uaccaguacg aguaucauga aaauauucuu acccacuccc ugcgcagcgc gaucauugac      660

cagucuaaga caggaaccac gaaaaucuuu cucaagacac auggcauuuu caccgccgau      720

aucaaaggga acagaaacga gcggaauaag caacuguuug agcuuagaug cggagcucug      780

gaacgcgccc ucacagcuga ggaacuggcc accgugaucg cggaggucaa cggccagauu      840

aauuccagcu cuggguaccu gggagccauc uccgaccgga gcaaagcucu cuucauuaac      900

caccagaccg ugggccagua ucugaugcaa cagcuugaca gagauccuaa uaagucccug      960

aagaaccagc ccuuuuaccg ccaagacuau cucgaugagu ucgaacagcu gugggagaca     1020

caggcccauu accacccuga acuuacggcu gcccugaaac acgagauccg ggacauuauc     1080

aucuuuuauc agagacgccu caagucucaa aaauggcugg uugccaauug ucccuucgaa     1140

caucggaagg ugugcccuaa aagcuccccg cuguuucagc acuucagaau uuggcagguc     1200

cucaacaaug ugaccgugac agucgauaac aagaccuacg uucugaauga ggaacaaaag     1260

agcauccuuu cuaaagagcu gcucuacaag gagaaaauuu ccaagcgcga cgcucugaaa     1320

augcuguucg ugaaggcgaa gggcguggau augaacuuug ccagccuuga aggggaccgg     1380

acaaugucua aucuccugaa agcugcccag gagaucguca gcauuuccgg acauggcgau     1440

uaugacuucg ggaagaugcc cgccgaugac auccacaaca ucauuagauc uguguuugga     1500

ggccuggggu ucgcuaccga cuggcucaau cuggauacgg acgccgauga ccuugaacag     1560

gagagccaga ugcgccugug gcaccucauc uacuccuuug agggagauaa cagcacaacc     1620

ggcgacacca aacugguuga acggcugaug ucucuucuca accugccuca ggaguaugcg     1680

agacuguucg cuaaugugca ucucgaagau gacuacugug gcauuuccag caaggcccug     1740

aaacggaucc uucccuuuau gaaggccggg cacauuuauu cugaggcuug cgcccuggcu     1800

ggauacaagc auuccgaaca aagccucaca cgcgaggaga acaaaaauag accucugaag     1860

gaucaccugg aacagcuucc caaaaacucc cuccggaauc cggucgugga gaagauccug     1920

aaccagaugg uuaauguggu caacucuauu aucgacaccu auggcagacc ugaugaaauc     1980

cgcauugagc uggcccggga acucaaaaau aacgcgcugg ggagacaggc caucuucgag     2040

gcuauuaauc gcaacacaaa ggagagcgaa agaauccaac agauucuuaa aaaggaguuu     2100

ggacugacgc augugucccg gaaugacauc auccgcuaca gacucuacca ggaacugucu     2160

ggcaacgggu auaagacccu guacagcaau auuuauaucc cccaagagaa acuuuucucc     2220

aaggaaauug auaucgagca cauuaucccu caggcccggc ucuuugacga cagcuucucu     2280

aacaaaacac uggaguaccg cgaugucaac aucaagaaau ccaauaccac agcuauugac     2340

uuugugagaa acgaauaugg cgagaaugaa guugagcagu acaagaagcg ggugaacgau     2400

cugcucaaaa gcggagccau cucucaggcg aaggccaaaa agcugaaaau gaccgaggcu     2460

gaaauuccca augacuucau cgagcgcgau cuuagaaaca cgcaauauau uucccggaag     2520

gcccaggaaa ugcugcgcga cgucgcuaga gugguuaccg ccacaaccgg cagcgugaca     2580

gaucggcuca gacaggacug gcagcugguc gaugugaugc aagagcugaa uugggacaag     2640

uacgaacgcc aggggcuuac cacggugauc aacggacggg auggccagca gaucagacgc     2700

auuacagacu ggaccaaacg gaaugaucac agacaucacg ccauggacgc ucugacaauc     2760

gcguucacca agcccgccua cauuucuuau cucaaccauc ugaaugcucg cagcgaggac     2820

gauauccggu ccauugagag acgcuuucuc gaacgggacg ccaacgggca ccugagauuc     2880

aucagcccua uugcccacaa uuuucggucu gaggcuaaac gccaucuuga aaugauccug     2940

guuuccauca aggccaaaaa caaggucgug accagaaaua acaacacaac gaagaccaaa     3000

ggaggccggc gcaaucggau ucaacucaca ccgagagggc agcugcacga ugcgaccgug     3060

uacggcagca uccugcuucc ccauacaaag accauugcug ucgacggaaa aaugacggcc     3120

gagaucauug gccaggugca aaacccuguu uucagacagg cccuguccca gcgccucgag     3180

gcuuguggga augaucccaa gaaagccuuu acaggaaaga acucucugga aaaagcgccu     3240

guguggaucg accaggcuca caccgagcau guccccgccg aagugaccau ccaacggcuc     3300

gagccgcagc acacaauuag acgcgccguu aauaaggauc ugaacaucaa uaaggugcuu     3360

gacaaaggca uucagcacau ccugcaggcu cggcucgaug ccuauggggg agacgcugcc     3420

aaggcguucg ccaaccugga uaauaacccu auuuggcuga aucaagaaaa aggcaucgcu     3480

aucaagagag ucaccacagc cgagaacauu agcggggaac cccuucauca caaacaugac     3540

aaggagggcc gcccuauccu ggacacgaau ggaaagccca ugccuuccga uuuugugcgg     3600

cucaugaaca aucaccaugu ugcuauuuuc caggacgccg auggcaaccu gcaggagcac     3660

aucgugucuu uuuacgaagc cgucgcuaga gugaccgagc ucgggcugcc cgugauugac     3720

aaagaauaua auaaggauaa aggauggcag uuccuuuuua cacugaagca aaacgaguac     3780

uucgucuuuc cgaacccugc gcagggcuuc aaucccgacg agaucgaucu gaccgacgaa     3840

cgcaacuaua gcagaauuuc ccccaaucuc uaccggguuc agaaaauguc uagcaaguac     3900

uauguguucc gccaccaucu ggagacaacc guggaagaua agaaagagcu ucaggggauc     3960

acguggaaga gaauccaauc ccugaacaau cucaaaggaa uugucaaggu gcggaucgac     4020

cacauuggcc gcaucguuca gauuggggaa uacuga                               4056


<210>  4190
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4190
auguucagcc agcaaaacaa ucuguacugc gagucccucu cuaagcugua uuacaacauc       60

accaaaaauu uucuuagcaa gaacgaagug uguagaauuu augagaucuu cuuuugcucc      120

auucugacau acuuccucaa aaaguauaau cacgacaucu uuaaaaaguc uagcacgaug      180

aagaccauuc ugggccuuga ucuggggguc acauccaucg gaugggccgu uaucgugacc      240

gacgaugaca acacacccau uaaaauccuc ggcauggggu cucggauuau cccucugagc      300

accgcugaua aggaagaguu ccagaaagga caggccauuu ccaagaauca gagccgcaca      360

aucgcgagaa cccaacggaa aggcuacaac cgcaagcagc ugagaaagga agaacucaaa      420

aagauucugg gggagcuugg aaucacgccg ucuaaagaac ugcucaaucu gcccuccauc      480

gagcuuugga agcugcgggc ugacgccgcc agcggcaacg cuccucucuc ugccgaacag      540

cugggccgca ucuuuuauau gcugaaucag aaaagagggu acaaguccgc ucggagcgag      600

gccaaccaag auaaaaagga caccgauuac guggcggccg ucaagggacg ccaucagcag      660

cucaaagaga agggccagac aauugggcaa cacuucuaua ccgaaaugcu gacacuugag      720

gaaaauucug gacauuuuca ggugaaaaac aauguuuacc ccagagaggc uuaucuggaa      780

gaguucaacg ccauuaucgc ugcccagaag ccucaacacg cguuucucac cgacgaugug      840

gucgaccggc ugcgcgauga ggugauuuuc uaccagagaa aacuuaaguc ccagaagggc      900

cugguuagca ucugugaauu ugagggguuc gaacggacgu ucuuugacaa cgugaaacag      960

aaggacaaaa caaucuucac cggacccaag gucgccccua aauccucucc gcucuuucaa     1020

cugugcaaga uuugggagac agugaauaac aucaaucuga aggugaaaaa caccgauggc     1080

agcaagaaua aaugggcuga cuaugucccc acccucgaac agaagcagga gauuaucgag     1140

caucuguuca aaaacgccgg cauuucccuu gaugcucugc gcaagauccu cggguuuaau     1200

aaggacgaaa ucuucguuaa caaacagauu gagaagggac ugcaaggcaa uacaacguac     1260

gccgaaaucc acaaaauucu uggggauagc aagccugagc ugcucggauu uaacaucaau     1320

auugaacccu cuaaacacuc cgcccugcug guggacaaga gaaccggcga gauccucgcu     1380

gagaaagaag ggcaacaggu gagcgcggag auugaaaagg agaacuucua ucagcugugg     1440

cauacaaucu acucuaucaa ggaucuugac gagugugccg cugcccugau uaaacgguuu     1500

aauaucgauc cuaacgucgc cgcucagcuc gccaagauug acuucagcaa aggagcguuu     1560

ggcaauaagu ccaacaaagc uaugagaaag auccugcccu accugaugca gggcuauaac     1620

uacgccgaug ccugcucucu ugcuggguau aaucacagcg acucccagac caagaaagaa     1680

aaggaggaaa acgugguuga cgauaaacuc aagcugcugg agaaaaauag ccuccgccag     1740

ccgguggucg aaaagauuau caaccaaaug auccaugugg uuaaugccau uaucgacaag     1800

uacggaaaac cugaugagau ucggguggag cuggcuagag accuuaagaa aucuaaggau     1860

gaacgcaacg aggccgaacu gcagaaugcg uccaacaaaa agcucaauga ggaagucgcc     1920

aaccggcuga aggagcuggg ccuucccgug acaaaaagau ucguccagaa guauaaauuu     1980

aucuucccug ugcgcgaccg gaagauuaaa gaggcucaac ucguuaauca guguaucuac     2040

ugcggggaaa ccuucaacgu gacggaggcc cugagcggag auaauuuuga cgucgaucac     2100

aucauuccca agucuaugcu guucgacgau ucccagacaa acaaagugcu cguucacaga     2160

aaguguaaug cuaaggacaa aagcaacaag accgccuaug auuuuaucgc ggccaaaggc     2220

gaaaacgagc uggcuauuua ccuugaccgc guggacgauu gguucaagag agggguccug     2280

uccuacggca aaaugcagag acucaaggug ucuuaugaac aauacgccga gcgcaagcag     2340

cugaaaaagg agaccgaagc ugacaucaaa cugugggaga auuuuauuga acgggaucuu     2400

agacagacac aguauaucgc caagaaaagc aaggaccucc ugaagaaaau cugcaacaau     2460

gugaccguua cagagggaaa gauuacggcc caacugcgga accucugggg cugggaugac     2520

guccugauga aucuucagcu gcccaaauac aaggcucucg ggcagaccga gaucaaagaa     2580

uggacauccg agcauggacg cagacugcac aagaaggaag agauuaccaa cuggacaaaa     2640

cgggaugacc auagacacca ugcgaucgau gcccuggugg uggcuuguac caagcagggc     2700

uucauucaac gcaucaauac gcuuaacucu agcgacacca aagaugccau ggaccgggaa     2760

auuaaggccg cuaaagagaa guauaaugag cucuacgaua agaaacugcu gacagaaacc     2820

aacgagcagg ucaagaauca cacagaaaga uuuaacaaac ucgagaccua ucugaagucc     2880

cagcaaccuc cgcuuuucac gacacgcuac guggaacagg aggccgacaa aaucuuuguu     2940

agcuucaagg cggggaaaaa ggcugccacc uacggaauca gaaagauuaa aaaggacggc     3000

aaaaaggugg ucgugcagga gaaaaucguu gugccccgcg gggcccugca cgaacagucu     3060

gucuauggca agauuaagac acaagagaaa gauaagccuc ucaaauaccu guuugaaaau     3120

cccgagacca ucguggacaa ccggauuaag auccugguua aagaaagacu ugauaaguuc     3180

gagggagacg ugaagaaagc ucugaccucc cucaagaaag agccucuguu uaucgauaau     3240

gaaaagaaca cacuccugga gaaagccgcg ugcuauacgg acgcuaccgu ccuuaaguac     3300

aagcugcagg cccucaaagc caaccaggug aaugauauug uggacgaagg cgucaagcgg     3360

aucguuaaag cucgccuggc ccagcauaag aacgagaaag aggcuuucaa ggauacagug     3420

ugguucaauc aagaaaaacg gauucccauc gugagcgucc ggguguuugc ccgcccggac     3480

gcgucuaagc uggccauuau caagaccgau gagaacaaua aaccuaucgg guucgcucuu     3540

acaggaaaca aucaccauau ugccaucuau aaggacaaca ccggcaaacu gguucagcac     3600

auuugcacgu uuugguccgc uguggaaaga aagaaauucg ggguccccac agugguuaag     3660

aauaccggaa cccucuggag cgagcugcuc cacaaggacc ugccuggcac auuucuugau     3720

aaacugcccc aggacgggcu cgaacuggag uacucucugc agcaaaacga gauguucgug     3780

cuuggccugc cuaaggagag cuuugaggau gccaucaaaa auaacgaccu cccccugcuc     3840

uccaagcauc uguaucuugu cuggagcauu ucugauaaug acuacugguu ccggcaccau     3900

cuggaaacca aaaacuccga gcucaagagc acaacgggag ccaaggaauc caaacgcuac     3960

uauagauuuc agucuguggg caccuucaca aaggagaauc cgaucaaagu ucagcugaac     4020

caccuggggg agauuaccaa gaucggagaa ccuuga                               4056


<210>  4191
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4191
augaagaaaa gaaaccggga ggaauacuau gugggccugg acaucgggac cagcuccguc       60

ggaugggccg uugcuacacc cgauuaccgc auuccuaagu ucagaggcca caaaaugugg      120

gggucucggc ucuuugccga gggaaagcgc gcggcugaaa gacggcaguu ccgcacgaau      180

agacggcgcc uggccagacg gcgcgacaga cuucaaaucc uggaggaacu cuuugccgag      240

gagauggcua aaguggauaa caccuucuuu augcggcuga aggaaagcaa guucuuucuu      300

gaggacaaaa cagugaagga aaaacauauu cuguucucca ccgauaaguu ugacgccgau      360

cgcuucuaua aagacaaucc gacaaucuac cacgucagaa agcggcucau ggaggaggaa      420

ccccaggaua uuagagagcu guuucuggcu gugcaucaca uccucaagua ucgcggccau      480

uuccuguuug ccgggucucu ugaacagaac ggagcgagca ucgaggaacu gguucggacc      540

cuccugggca aacagggccu ugccauucug gacgauacaa ucgacuccaa ugagauggug      600

aacauucuca agaauaccgg ggagacgaaa ucugauagaa agaaagacgu ccaagaacug      660

uggaacccuc ugaaggacaa agugaagaag gcucaggccg cugagauggc cggacucguu      720

cugggccuua aaagcgauau cuccaagcug uucgcgaaua gcgacuacaa agaucucgac      780

ucuuccauua agaacaucaa uuucagcucu ggggauuaug aagagaaacg cgacgauuac      840

gccaacucca uuggagacga uaucgugcug cuugacacca ugaaggcucu guacgauaca      900

cucauccuga gccagauucu gcagggcaag cggucuaucu ccgccgcuaa agucgaagac      960

uauaauaccc acaaggagca acucagagcc cugaaagcca uucuuaagcc ccacaaacgc     1020

cuguacaacg agauguuucg ggaaaauaac aagaaggacc ucagcaacua uguggcuuac     1080

aucuccucuu auagcgaggg gaaaagagug aaucgcgaag gauucuacaa guauguccag     1140

gcgguucuga aacaggugga ugacuccgau agcaagcagg ccauucuuga ggcuauccgg     1200

cuggaaacau uucucccucu gcugagagug aaagacaacg ccgucauccc ccaucaaauu     1260

caccaggccg agcucgaggc uauccuggaa accgcccgca agaaguacaa uuuccuuaac     1320

gaugugucug acggcguuuc cgugaaagau aagauuaucc agcuguuuac guuccggauu     1380

ccuuacuaug ucggcccgcu caauccccau agcggggaga caguggcgga cagaaaacaa     1440

ggaaccggcu ggguugugcg gaagcagcgc gggaaagucu acccuuggaa cuuugcugau     1500

augguggacg ucaaggccuc ugccgaggcu uucaucacaa aucugaccaa caaaugcacc     1560

uaucugaucg gagaagaugu gcuucccaag agaagccucc uguacuccga guuuaugcug     1620

cucaaugaac ugaacaugau uaaguaugac ggccacgagc uuccuguugc caucaaacag     1680

cuguacauug aacagguguu caagcaagag cacaaaaagc agacaaaagg gcggguccuc     1740

gauuuucuga agagaaaugg acugaucacg ggcucugcua ccgccgugau uacaggcaug     1800

gacgacgcga ucaaggggga ucuucagagc uaucgcgaca ugguucggau uuucggagcc     1860

ggcuuugauc auuccauggc ugaaagaauc auccgcuggg ugacccucuu cggggaggcc     1920

aaagacauuc uggagaacaa gaucagagaa gucuacggaa auagcauuuc ugauucccag     1980

augcgggcua ucaaaaagcu gaaauacgcc gacuggggcc gccucagcca agcguuccug     2040

auugccguga agggguguga uaagucuggc guggagagaa acaucaucac augccuucgg     2100

gcuuccagcc agaaucugau ggaaauucuc uccgaccagg gaaccuauau caaacagauu     2160

aaggccugga accaccaaca uacgacaaac augauggaua cccuggacua ccagcugguu     2220

gaugaccuuu augucgcucc cucugugaaa cgcagcaucu ggcagucccu cuguauugug     2280

gaggaacugg ucaagaucau gggccaggcc ccgaaaaaga ucuuugugga gguuaccaga     2340

acaaagcaag ccgagaaaac caagaaagau ucucggaaga aacgccuggu ggaacucuac     2400

acagcuauug ggaaggaggg agcggccugg aaaaaggaaa ucgaggcuca ccaggccggc     2460

gacuucagac ggaagaaacu guaucuuuac uauacgcaga uggggcgcug cauguacagc     2520

aaugaaccua uugccaugga cgcucuguuu accuccgccu acgauaucga ccauauuuau     2580

ccccaaagcc ucacaaagga uggaucuugg gacaaccugg uccuggugaa aucccagcuu     2640

aauaaggaga aaagcgauca guacccucuc agacccgcga uccgggagaa gaacaaggcu     2700

cacuggcagu aucuguguag agccggcuuc aucucugaaa agaaguacga gcgccugaug     2760

cggaccacag aacucaccgc cgaggagcug uccggguuua uugcuagaca acuuguugaa     2820

acgagccagg ccaccaaagc ggugacaggc cugcucgagc gcaucuauac caauacagaa     2880

auuugcuucg ucaaggcuga gaccgugucu caguuucggc accagcauaa cuucaucaaa     2940

auuagagaac ugaauaacua ccaccaugcc aaggacgccu aucugaauau cguuguggga     3000

aacgucuacc acgagaaguu uacgaaaaau cccugggagu ucauugcucg cagcacagaa     3060

cggagaccuu acaaccuuaa ucgcauguau gauuuugacc ugcucaagca aggccgggug     3120

aucuggcaag ugccgaaauc ccuggagacc guccaggcca ugaugaaaag caacgauguu     3180

agaaucacca aaaagcuccu ggugcagaag ggggaacuuu acgacgcuac aauuaccaaa     3240

gcccacaagg cgaaagaggg aacauauuuc ccccugaagg gcgccgaugc ucggcuccgc     3300

gacgugcaua gauacggggg auaugagaaa aucaagauug ccuacuauuc ugucuuuucc     3360

ugucgggaaa aggcuagcaa uacgauccac acccugauua ucccuauccc cguguacaaa     3420

gccggccugc uucagacaga ggccgaccug accgcuuacg cguggcagac aaagcagaaa     3480

accaagccua uuaaagauau cuauuccgcc auuacgccca ucuacaagaa acucugcauu     3540

aacacaguug ugaagaucga cggguucaag uauuuccugg gcggaaaaac cggcgauuac     3600

auguggcucg acccggcuau cccugucgug cuggaugaac aagccgccgc uuaucuuaag     3660

gccauuucua aaguugggcg cgcggacgau guggagaaca ccgcucaggc caucgaccug     3720

auuacagccg aagcuaauga ggccuuuuac gauguccucc ugaagaaaau ggaagcgaag     3780

aucuucgcua aggccuauca gaccauugug gagaaacugg ccagcgagac agcuagagaa     3840

cgguuuagag agcuuuccau cggagcccag uguucuugcc ugcucgaaau ucugaaccuc     3900

cuggcuaaua acaagacgac cuucacauuu uucaaagaca agaugggcgu uaccaugagc     3960

cgccggaaac aacccaugga ucuuacaaag cuggaggagu guaucgugua cgaaaccucc     4020

aucacggggc uguacgagaa ggccgucucu cuccuguga                            4059


<210>  4192
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4192
augggcaaga gauaccacau cgggcuggac auuggaacca gcuccaucgg cugggccgug       60

auggaugacg auuucaaaau uaugcggguc aaggggaaaa agggaaucgg cguucgccuc      120

uuugaggaag ggaaaacagc ugccgagaga cggggacauc gcacgaccag acggcgcuau      180

ucuagacgga aguggcgccu gaaccuucug gaagagauuu ucgaccccga aaucgcgaag      240

guggauccua cauuuuucgc uagacucaaa gagagcaauc uguccccgaa ggacgaucgg      300

aaacaguacu cuggcaaccu uaucuuuccc gagaaggcca aagaagagau ucgcgaaugg      360

accgagaagu auccuacaau cuaccaccug agaaaugccc ucaugaccga gaaccggcaa      420

uucgacauuc gcgaaguguu ucuggcucug caucacaucg ucaaguauag aggcaauuuc      480

cucuuuaacg ggaauuucca gacaggagag gugaaccuga gcgaucgguu ugacgauauu      540

aauuccgccu ucaucucugc uugcggcgaa gaggaagcgc cccagauucc uagcgagcuu      600

uacaacgaca uccaggagcu gcucuuugau aaaaauacca gaaagcugga caaccaaaaa      660

cagcuugcca cgaagcuguc ccaggacaau gauaaagacu ggagcaccca guucucuaag      720

gcccucuucg gguauaaaac aaaguuuaag guugugcugc aauccaacga agaucuggac      780

gauuuccagc ucgacagcga ugacgcugau gacaaaaucg ccucuauuuc cgagcagauc      840

gaugacgacc gcggaagcau ucuggauaau aucaagauuc uuuacucucg gaucacccug      900

aacgaaaucg uccccgacgg caaaacacuc ucccagagca ugauugagaa guacaaccug      960

cauagagauc accuuaaaga aaucaaggac caacugaagc acaccgauaa uugggugaaa     1020

uuugacgauc ucuauuccca guacguuaac gggaagcugg agucugaccg ccauaaaaag     1080

cugagcuccg augacuucua uaaaaaggug cucacgcugu uugauggaga gcuuaagaca     1140

cgggucaaag aauggcagga gaagggcgag uuccugccua aacaaagaac cggcagcaau     1200

ggggugauuc cgcaccagcu ccaucagaac gaacuggacc agaucauuga aaaucaaucu     1260

aaguacuauc ccuggcuugc uacacugaac ccuaaugaga aacgccagcg gguggccaag     1320

uacaagcucu ccgagcuggu cgcguuuaga auccccuauu acguuggacc uauggugacc     1380

aaagaucaga gcgacuucgc cuggauggug cgcaagucug acgaaaaaga gaucaccccc     1440

uggaacuuug aucaggucgu ggaccgggau aagagcgcug aagaguucau uaauagaaug     1500

acaacgaaag acaccuaccu gaucggcgag gauguucucc cggccaaguc ccugcuuuau     1560

gaaaaauuua cagugcugaa cgagcucaau aacauucgca ucaaugggaa gccucugacc     1620

acagacgaaa agcaccaagu cuaccgggau cuguuccaga acgcuaaauc uauuagcacc     1680

aagaaauuuc acaauuaucu uguguguaag cucaacaaac ccaaccaucu gaauaucacg     1740

ggauucuccg accugacaaa guucaacagc ucucucgauu ccuacauuga ccugaagaaa     1800

aucuuugccg aucagaucga cgaucagagc cuucaagacg acuucgagaa gaugauuaga     1860

uggucuaccg uuuuugaaga uaaacagauc uucgagcgga agcuguccga gauuaauugg     1920

cucaccgacc aggaacgcag cgagguggcc aaacugagau aucggggcug ggggagacug     1980

uccaagcgcc uucucgcugg aauccacgau gaaaacggcc agcggauuau cgacgcgcug     2040

uggaacucuc cuaagaauuu uaugcaaauc caggccgagg ucgcuuucaa uaaacagauu     2100

aacgaucuga auacaaagga acucagcgac aacaaugugg aggauguccu ggccgacgcc     2160

uacaccucuc cccaaaacaa aaaggcuauc agacaggugc uucugguugu ggaggauauu     2220

cagaaagcca ugcauggcga cgcgcccaca uccaucagcc ucgaauuugc ucgcgaggaa     2280

caggagucca gcagaacgcg gucucgcaga gccgcccugu ccgcuaccua ucggcaaauu     2340

agcgaugaaa ucgucucugc cacagugaag ggggagcugg gaaaggcuac cuccaucagc     2400

gaccgccuuu accucuacuu cacacagggc gggagagaca uguauaccgg agagccuauu     2460

aauaucgaug aaauuucucg gggcuacgag aucgaccaca uucugccgca gagcuuuauc     2520

aaagaugacu cccuggauaa ccgcguucuc gugaaggggg ccaucaauaa cggcaaaagc     2580

gcgaacuaug ucauggacca gucuugggcc cccgauagac uggcuuggug gaagaaacuu     2640

cuggacaauc ggcucauuuc ccgcaagaaa uaccaaagcc ugacgcugac cccuggaaag     2700

aucaacaagu auucccagaa uggcuucauu aacagacagc uuguggaaac aagacaggug     2760

aucaaacucg uugccaauau ucugaacgau caauacucua augacgauac caagaucauu     2820

gucgugaaag cugcccugaa cagccagcuc cggggguuuc uggagcuuuu caagaaucgc     2880

aacaucaaug acuaucauca cggaauggau gcguaccugu ccacauuuau cggcaacuac     2940

cucuauaaaa aguaccccaa gcugcggucu uauuucgugu acggggaguu caaaaaguuu     3000

agcggaaaac agaaucugaa guccuucaac uuucuucacg accuggaaga cgauucuauu     3060

aauaucauua gcaacgacga uggcgagauc gucgggaacc aucacgaaau gauugaggaa     3120

cucgagaaag ccuaucaaua caaguauaug cuggugucca gagagguuuu cgaaaagacc     3180

ggcgaccucu uuaaugugac ggucuacagc cgcaaagcua agaaaacacu gaucccuauc     3240

aagcaggaua aacccauuaa caucuacgga ggcuauucug ggaauaacga cgccuacaug     3300

gcuauuguga aacuuccggg aaagaagggc aagccugaua ccuauaaaau cguugggauu     3360

ccccugcggu ucgccggaga gcucgaaaga gccaagcagc ugggcgaguc cgcuuaccgc     3420

gaaaaacuga gcgcggugau cacaccucag uuuaccaagg agaagaaaga ccggaagacc     3480

ggggagauca caaaagucgu ggaugacuuc gaaguugugc uugauagagu ccccuaucgc     3540

caacuguuua aggacucuga uaaaugguuc augcucggcu ccgccgcuua ccaguauaau     3600

gccaagcagc uggugcucaa caaugacgac gugaaacugc uuaacgagca gggacggaag     3660

aagcuggaac ucccuaauga gaaacugagc ucugcccuga acgaugucuu ugaccauauu     3720

auccaaauua ugaacuccaa guucggccuu uacgauaaaa auggguucag aacgaagcug     3780

accgaagcug ccagcaucuu uuacgaccuc ccccugcggg cggaguauaa uggaaacaaa     3840

aagguuacag auggcaagcu cgaggugcug gacaauauuc uuaucgggcu gcacgcugau     3900

ccguccuucg gaaaccugaa acagcucaau augagcaccc cuuuuggcca gaugcagaca     3960

aaagggggcg ugguccugaa cggaaacucu acccuuauuu accaaagccc cacgggccug     4020

uucgaacgca gagugaagcu caaggaccug uccaaauga                            4059


<210>  4193
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4193
augaagaaaa uccugggccu cgaccugggg accacaagca uuggaugggc cuucaucaac       60

gagggcgaaa augauaacga gacguccucu auuaucaaug cuggggugag aauugucccc      120

cuuaccacag acgaagaggc cgauuuuaag aaaggaaaca ccaucagcau caaugcggac      180

cggacacuga agcgcggcgc uagacggggg cuccagcgcu ucaaacaaag acgggaugcc      240

cugcuucagu ccuuucgcgg aauuaacuuc aucucuagca auuacguuua ugccgaaacc      300

ggcaagcaga caaccuuuuc cucuuacaag cugagagcua aaagcgccac ggagcucgug      360

accaaggagg aacuggugca gguccugcuc augcugaaca aaaagcgggg cuauaaaucc      420

agccgcaagg cuaagacagc cgaggaaggg gacgcgauug auggaaugaa aaucgccaag      480

gagauuuucg agaauaaccu uaccccuggc caaugggugu acaauucucu guccaucggg      540

ggaaaauuua uuccggacuu cuauagaagc gaucuccaga aggaaguuga gaaaaucgug      600

cgguuucaga accaguucca ccccgaacaa augaaugaga agcugcuuga aaacauccag      660

ggcaaagcuc gcacagucac cucucaguac uuuucccgga uucugaauau cccucucgcc      720

gagaacaagg ggacgagaga ggaaacaaag cugcagcauu auaaauggcg caacgaggcu      780

cugaccgagg aacucgaccu gaaggugcuu gccuucauua ucacagaaau uaauaaccaa      840

aucaaucaga gcaacggaua ccugggcgcg aucucugaua gauccaaaga gcucuacuuu      900

aaucaggaga ccguuggcca auuccaguau aaccagcuga aggaaaaugc ccacaccaaa      960

cuuaagaacc agguguucua ccggcaagac uaucuggaug aguuugaaaa gauuugggag     1020

acacagagca aauucuaccc cgaacucaau gacgagcuga aguccgaggu cagagacauc     1080

acgaucuuuu aucagcgcaa acugaagucu cagaaacauc ucgugagcuu cugcgaauuu     1140

gagaaggggc acaaggcuau uccuaaaucc agcccccugu uccaagaauu ucggauuugg     1200

cagaacauca auaacaucgu ggucaagaau gagaccacaa aagagccuuu ccuucuggga     1260

gaagaucuca agaaaguuau ugcccaggag cugucuuuua aggaauccau gaccgacagc     1320

cagcuucuga cauucuguga ucucaagaaa ggcacccaua cggugaacuu uaagaaaauc     1380

gaggggaaua gaacaaacac cucucuguuc aaggcuuuug aaaagauucu ggugcucgag     1440

ggauacgacg agcuggaucu uagcaagcug gacgccuccg auaucaaauc uauuaucaag     1500

agcgccuucu ccgaccucga aauuagcacc gcuauccugg auuucgaccc gacaauccaa     1560

ggcaacgauu augacaagca gucuuacuac cagcuguggc accuucucua uuccagcgag     1620

gaagacgauu uucugaaaaa gucucugaug gacaaauucg gguuuaagga gucccaccuc     1680

cccuuccugc uuaauauuaa ccugcaggcg gauuacggaa gccucuccgc caaagcuauc     1740

aagaaggucc ugccucaucu gauggacggc cacauuuaug auaaagccug cacccuugug     1800

ggcuacaauc auucuagcuc ccucacaacg gaccaaaaca augaacgcgu ucugaaggau     1860

acccuggagc uccugaaaaa gaacucucuu cggaaucccg uggucgagaa aauccugaac     1920

cagaugauua augugaucaa cgccaucauu aaggacccug cuauggggaa gcccgaugaa     1980

aucagaguug agcucgcccg cgaacugaaa aauaacaaug agcagcggag cgaaaugaca     2040

aaggcgauua acaaauccac cacagagcac gagcaaauca gaaagcugcu ucacaccgaa     2100

uuugggauuc aucgcgugac gcggaaugac aucaucagau auaaacucug gaaggagugu     2160

gauggcauua gccuguacac cgggaagccg aucgaaccuu cuaaacuguu cacaaaggac     2220

uaugauauug agcacaucau ucccaaaucc cgccucuuug acgacagcuu cucuaacaag     2280

accaucugcg agcggcagcu gaacaucgau aaauccaaua agacagcuua cagcuuuuuc     2340

caggaaaaac uuucugccga ggaauuugac caguucgaga agagagucaa gagccuguuu     2400

ggaaaaauuu cccggaccaa gcaaaacaaa cuccugaugg ccaauaacga aaucccugag     2460

ggcuucauug aucgccagcu gagagagacg caguacaucg cuaagaaagc caaggaaauu     2520

cuucucgagg ugagccggaa ugucacagcu accaucgggu cugugacaga caagcugaga     2580

gaugacuggg aacugguuga ugugaugaaa gagcucaacu gggaaaagua ugacaaacug     2640

ggccuuaccu acauugagga gggaaagaau ggcgaacgcc uguauaaaau caaggauugg     2700

accaagcgga acgaccauag acaccaugcc auggaugcga ucacagucgc cuucacgaaa     2760

cccgcuuaca uucaguaucu caauaaccug aaugcccgca ccaacacaga caagaaagga     2820

aauuccauca uuggaaucga gaacaaguac cugaaaagag auaagaauaa caaacuucgg     2880

uuuauugaac ccaucccuaa cuuccgcgag guggcuaaaa agcaacugga aagcauccuc     2940

auuuccuaua aagccaagaa uaaaguugug accaagaaca agaaugucac caaaaagucu     3000

aacggcacaa aucagaaaau ccagcugacg ccgagagggc agcuccacaa ggagacagug     3060

uacggaaaac ugcaacagua cgugaccaag gaggagaaaa uuaacgcgaa uuuuaccccc     3120

gaauauguua caaaggucgc caagaaagaa uaccgggagg cccuucugaa gcgccucauc     3180

gagaacgaaa augacccuaa aaaggcuuuc accggcaaaa acgcucugag caagaauccc     3240

auuuauaucu cccuggagaa caauauccuu gugccugaaa aggugaaaau ugucuggcug     3300

aagucugacu acacaaucag aaaagauauu acgcccgacc ucaagaucga uaaagugauu     3360

gacaucgggc ugaagaccau ucuccagaac cggcugaaug aguuuaacgg cgauacaaag     3420

aaagccuucg uuaaucuuga cgaaaacccg aucuggcaaa acaaggagaa aggaaucgcc     3480

auuaagcgcg ugaccaucag cggcgucucc aaugcucagg cgcugcacac caaaaaggau     3540

caucucggga acgagauucu ggacgaaaau ggacagccuc ugcccgugga uuuuguuucu     3600

acaggcaaca aucaccaugu ggccaucuau agagacgaga aggggaaccu ucaggaagag     3660

gucgugagcu ucuuugaagc uguuacgcgg aauaaccugg gacucuccau uaucaauaaa     3720

aaccacgaga agggcuggga guuccuguuu agcaugaaac aaaaugaguu cuucaucuuu     3780

ccuaccgaug gguucaaccc ccacgaaauu gaccuccuga auacagaaaa cuaccaucuu     3840

aucucucgca auauguuuag agugcagaag auugccacca aaaacuauau guucaaccac     3900

caucuggaga caaaguccgu cgacggcgaa augcugaaaa ccaagaagca gcucagcaaa     3960

aucucuuacc acuuuauuca gacaccuucc uaccugaauu auaucaucaa gauucggauc     4020

aaccaucuug gacaaauugu gcagcugggc gaguacuga                            4059


<210>  4194
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4194
augaagcagg acuacuauau cggccuggau auggggaccg agagcguggg augggccguc       60

acagacguug auuacaaccu cauuaaaaag agaggccaag acuauugggg aguguaccug      120

uucgaugaag cucagacggc ccaggggcgg cgcgcguuua gaaccaaucg gcgcagacuu      180

gcucgggugc gccacagacu gaaccuccug caggagcuuu ucgacuccga aaucucugcc      240

aaagauuuug gcuucuuuua ucggcuggag aauagcaagu acuucgacga agauaaaccc      300

gagauugccc gcgggaaguu ucuccuguuc aacgacaagg auuuuggaga caaagaguau      360

uacaagaauu acccuacaau cuuccaucug agaaacgcuc uccggaccca agaaauuaaa      420

gauguccgcc ugcuuuaucu ggccgugcac cauauccuca agaauagagg ccacuuucug      480

uucggcgagc aggacuuuaa cguuaaugac gcuuccgaug ugucugggga auuugagaaa      540

aucaaccggu uccuuagcga gaaugacgga gccggcuucg aucgcgaaaa gauuccggag      600

aucuucgaaa cacugugcaa cgagaaucuc gcgaagagag aacggaaaga gaagauucug      660

gagcuggccg ggaacuccaa aaauaagcuc cugggagaag cugucaacgc cgugguuggc      720

cuuaacuuuu cucugagcaa aauguucggg gaggacagag auuuucaggg agaaaauaag      780

auguccuuca ccgacagcuc uuuugaggaa aaacucgcua aggcccgcau ggcgcuggau      840

gacgaugagg ccgacuuccu ugagacacug aagaaagugc aguccgucgc uagccuccau      900

aacauccugc acggcaagga auuuauuucu gaggccaaaa ccgaggcuua cgaaacgcac      960

cggagagauc uguccgaacu caagaaauau gugcgcgaga auugucccga gaaguacgcc     1020

cugguguucc ggagacaagu caagaaaggc aagaaaaaca ccagcggggc cgacgaaaau     1080

gagaaggacg auaaaaaggu uaacaauuau gcugcguaca ucggaaugga caagggcaaa     1140

ggguuugaaa agugcaaaaa ggaggauuuc uaugccuuuc uuaaaaagga gauuggaauc     1200

aacgacgaau cucugcucgc uaagaucgau gagggcauuu uccugccuaa acagaucucc     1260

gccgaaaauu gugacauucc cuaccaggug caucuugccg agcugaaggc uauccucgcc     1320

aacgcggaaa auuacuuccc uuuucugaaa cagaaggaug agaacugcga gaugacagug     1380

agcgagaaaa uuaucucccu gaugaaguuc cgcguccccu auuacguggg gccuuuuacc     1440

gacaaagaaa cacgguucuc uuggaugauc aagaaggaag gacucagcac ccagaaaauu     1500

acgccgugga auuuugauga cguuguggau agagacgcuu ccgagaagaa auucaucgaa     1560

cgcaugacaa acuuuuguac cuaucugaca ggcgagaagg uccuucccgc cagcucuuuc     1620

cuguacuccg aguaugccuu ucucaaugaa cugaacaacc ugaaagugaa uggcaccaag     1680

gaucuucggg uuaagaacau ucucuucgac uuugcuaaaa ccaauaagaa acugacacug     1740

aagaaaugcu gugagcagcu cgugaagaac gggcugcuuc cugaaggaag caaggccaga     1800

gacgucuucu cuggcacgga uggggacuuu aaaaauagcc uguccucuua caucgauuuc     1860

aagcgcauua ucggagacaa agcugaucuc uaucgggaca ugugcgagaa gaucauucug     1920

uggaucaccg ugauuagcga uaaagaaaga cuggucaaga ucauuaagga caaauacggc     1980

acagugcuuu ccgaugagga gaucaagggg cucaaagccc ugaacuacac caaguggggc     2040

cggcugagcg aaaaacuccu ggacggaauu uucgagacag auucugaagg cgagguuggg     2100

aaugugucca ucaucgagaa gaugcgcacc gacacggaaa acuuuaugga gcuucugagc     2160

aauaaacaca acuauaagag agcgauugaa cucgccaauu cugaguccau cgucgaugaa     2220

cgggugacau acagagcugu ugaggagcug uauuguagcc ccgccgugaa gcgcuccauu     2280

uggucugcuc ugaaaaucgu ccgggaaauu gugaaggugc agggacauag cccuaaaaag     2340

aucuucguug agaccaccag agaaaaaccc gacgucacac gcaagggcca aagaaccgug     2400

ucccggaagc agcagcuucu cgacaaauac cgcgagaacc ugagaaauga aacagagcug     2460

aucaagaaaa uugaggaaac ggaggauucu aaguuuaaca gcgacaaacu cuucuuuuau     2520

uucacccaga uggggaagga ugccuacaca ggagaaaaga ucgaguuuga cgauguguuc     2580

aaaaccgaaa aguaugacau ugaucacauc uacccgcggu ccaaaauuaa ggacgauagc     2640

cuggacaauc gcguccuugu gagacggaaa aucaaccaau cuguuaagaa ggauuccuac     2700

ccucugagcg cggagauccg caacagaaug gagggccucu ggcggcagcu gcgcgacaaa     2760

aagcugauuu cugacgaaaa auuuuauaga cuuacacggu ccacgccccu caccgagaag     2820

gaaugcgggg auuucaucaa ugcccagcug auugcuacca gccagucugc caaagcugcc     2880

auccaacugc ucaagagaau gcugccugag agcgagauug uguacuccaa ggccggcaac     2940

gucaaugaau uuaaaaagcg ccuugaguuc gugaaaaucc gggaacugaa cgaccuccac     3000

caugcuaagg augcguauuu caauaucguu gugggaaacg ucuuuaauac aaaauucaac     3060

cacaaucccg ccguguuuca gagaagcaac ggcaccggga auuacgacuu cgagcgccug     3120

uaugauuuug acauuacaaa cgcuuggaag aaaggagaug aaaaucggau cagaucuguu     3180

gccgagaaga acaccugucg cgugguccgg uucuccaaga cggagaaagg ccaguuuuac     3240

gacgccacaa uuuaucccgc ugggaagaau gauaaacugg ccccugugaa gggaaaagcg     3300

ccgcuuaacg acaccagcaa guacggcggg uacgauaagg aaacaaccgc uuauuucaug     3360

cuggugaaau ccagagacaa gaaaaagaaa gauaagaccc uccugucucu cgagugcguc     3420

acaguucugg ccgacaagaa cuuucgcacg gccgaagaca aacuuaagua cugcgagaaa     3480

gaacugggcc ucaaggagcc cgagauccug auugagaaaa ucaagcugaa uacccuuuuc     3540

aagauuaacg gaagcuaugc uuggauccgg ggcagaacaa augaucggcu ggugcucugu     3600

aacgccaauc agcuguggcu cgacaacggg uccgcgaaag aacugaagaa aaucucuaau     3660

cauuuucgcg augugaagaa auacaacaau aaggaccuuc cugucagaga agaggaguuc     3720

agcaaagaac aaaaccuggc ucucuaugau cuguuuaccg agaagcuggg aggcaagauu     3780

uacgccgggc uucccgugca gggacagauc aaaaaucugg aaaagggccg ggacaaauuc     3840

gaggcccucg aacugaagga ucaacucuuc guucugaugg agauucuuaa auuuaugcaa     3900

ugcaacucca ugacaucuaa uaucaagcug auugggggcg cugagaacgc cggaaagaau     3960

cgcaucagca aacugaucca ggacuccgau gugaagauga uuuucuguag cccuaccggc     4020

uauuuuugca aagucgugga cuucaagaga uuucucuga                            4059


<210>  4195
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4195
augaagaaaa uccugggccu cgaccugggg accacaagca uuggaugggc cuucaucaag       60

gagcccgaaa aagauguggu cggcuccgag auuguugaca ugggggugag aaucgugccu      120

cuuucuagcg augaagagaa cgacuuugcu aagggaaaua cgauuuccau caacgccgau      180

cggacccuga aacgcggcgc gagacggaau cuccagcgcu ucaagcaaag acggaacgcu      240

cugcuugaaa ucuuuaagga gaaaaagcug auuucuacaa auuucaaaua cgccgaggac      300

gggccgagcu ccaccuuuuc uacacucaac cugcgcgcca aggcugccaa agaaaagauc      360

gagcugcagg aucucgucaa ggugcugcuu cagauuaaua aaaagagagg auauaaaagc      420

ucccggaagg cuaaaagcga agaggacgau ggcucugcca ucgacuccau gggcauugcg      480

aaggagcugu acgaaaacga ucucaccccc gggcaguggg uuuaugaggc ccugcaaaaa      540

ggacgcaaga augugccuga cuucuacaga agcgaucuuc aggaagaguu uaagaaaauc      600

gucaacuauc agucugaguu cuuucccgac auuuucaaug cuuccuuugu ggaagacugg      660

augggcaagg ccagcacacc uaccaaacag uacuucaaca agaaaggggu ucaacuggcu      720

gagaauaagg gaaagcggga ggaacgcaga cuccaggaau auaaauggcg ggccgaggcg      780

gugaacuuua agaucgaucu gucugaaauc gcccugauuc ucucccagau caauagccag      840

auuuccaacu cuagcggcua ccugggggcu aucuccgacc gcagcaaaga gcuuuacuuc      900

aagaaccuga cggucggaca auaucucuac cagcagauua aaaagaaucc ccacaccaga      960

cugaagggcc aaguguucua ucggcaggau uaccuugacg aguuugaaag aaucuggucu     1020

gugcagucca gcuucuaucc ucagcugaac gaugcccuca aacgcgaggu ccgggacauc     1080

acaauuuucu uccaaagacg ccugaagucu cagaaacauc ugaucagcaa uugcgaauuu     1140

gaggaucacc auaagguugu gccgaaaucc caccccgugu uccaggaauu ucggauuugg     1200

cagaaccuca auaaccugcu ucugaucaag aaggacaauc ucaacgagaa auucgaucug     1260

gagcuugaau cuaagauugc ucuggccaau gagcucgccu uuaaaagaga acugaacguc     1320

aaggacgcuc ugaaaauccu cggccugaag ccuaaugagu gggaguucaa cuuuaccaaa     1380

aucgaaggga aucgcacaaa ccaagcguuc uuugaugccu ucgcuaagau uaucgagcuu     1440

gaagacggag agcccauuga ccugggcgau cucaaggccg acgauauccu ggaccaguuc     1500

agcgaagccu uucugcggau ugggaucgau accgagcuuc uccagguuaa uuccgacauu     1560

gagggagcug aauacgagaa acagagcuau auccaauucu ggcaucugcu guacucuucc     1620

gaagaugacc agaagcucaa acugaaccuu aucagaaagu uuggcuucaa accugagcac     1680

gccaagauuc uggcgagcau cucucuccag gaugaccacg cuucccugag cucccgcgcc     1740

auuaagaaaa uccugcccca ucuucagucu gggcucauuu acgauaaggc cuguacguau     1800

gcuggauaca accacagcuc cucuuuuaca aaagacgaaa augagaagcg ggagcugaga     1860

gccgaacugg agcuccugaa aaagaacagc cuucgcaauc cgguggugga aaagauccug     1920

aaccaaauga ucaaugucgu gaacgcuauu cucaaagauc cugagcuggg ccggcccgac     1980

gaaaucagag uugagauggc ccgggagcug aaggcgaaug ccgaacagcg caaaaacaug     2040

accuccaaua uugcuagcgc cacaagagac caugauaagu aucgggagau ccuuaaaucu     2100

gaauuuggcc ucaagagagu gaccaagaac gaccugcugc gcuacaaacu cuggcuggag     2160

accgauggga uuucccuuua uacaggaaag ccuaucgagg cuagcaaacu guuuucuaag     2220

gaauacgaca ucgagcacau uauccccaaa gccagacucu ucgaugacuc cuuuagcaau     2280

aagacgauuu gcgaaagaca gcugaacauc gauaaagcga augucaccgc cuucucuuuu     2340

cugcaaaaca aacuuagcgc ugacgaguuc gaacaguauc agucccgcgu caagagccuc     2400

uacggcaaac ugucuaaggc caaaauucag aagcugcuca uggcuaauga uaaaaucccc     2460

gaggacuuua ucgccagaca acugcaggag acacgguaca uuuccaagaa ggccaaagaa     2520

auccuuuucg agauuagccg cagagugucc gugaccacag ggaccaucac ggauaagcug     2580

cgggaagacu ggggacucgu ugagauuaug aaagaacuga acugggagaa guaugauaaa     2640

cugggccuua cauacaccau cgaggggaag cacggcgaac gccugaacaa gauuaaagac     2700

uggucuaaga gaaaugacca ucggcaccau gcuauggaug cgcucaccgu ggcccugaca     2760

aaaccugcuu auauccagua ccucaacaau cugaacgcca agggacuuaa uaacaaaaag     2820

ggcaccgagg ucuucgccau cgaacagaag uaucugaaac gcgagaaugg gaagcucugu     2880

uuuauuccgc ccaucgaaaa cauuagaagc gaggcuaaaa agcaccuguc ccggauccug     2940

gugucuuaca aagccaagaa uaagguugug acaauuaaca aaaauaagac gaaaagcaag     3000

gcgggacuua acgagcaaau cgcucugacc ccucgcggcc agcuccauaa agaaacaguc     3060

uaugggaagu ccuuccacua cucuaccaaa uuugagaaga ucggagccag cuucaaugug     3120

cagaagauua acacaguggc caaaaaggaa gagagagagg cucugcucaa acggcuggcc     3180

gaaaauggca acgaccccaa gaaagcguuu accgggaaga acacgcuuaa uaagaugccu     3240

aucuaccugg aucucggcaa aaacauuaag cuguccgaga aaguuaagac cgucgugcug     3300

gaacagaauu auacaaucag aaagaacauu gaccccgauc uuaaggugga caaggucauc     3360

gaugugggaa ucaaacgcau ucuggagagc cggcucgaag aguucggcgg gaaugcuaag     3420

cuggccuuuu cuaaccucga ggaaaauccg aucuggcuga acaaagagaa gggaauuucc     3480

aucaaaagag uuaagauuag cggcgugucu aaugucgaau cccuucacgu gaagaaagac     3540

cauuucgggg agccuauccu ggaucaagaa ggaaacgaga uucccguuga cuuugugagc     3600

accggcaaua accaccaugu cgccaucuac gaggaugaaa augggaaccu gcaggaggaa     3660

gugguuucuu ucuuugaggc ugugguccgc cagaaucaag gccucccuau cauuaagaaa     3720

aaccacacac ugggauggaa guuccuuuuc acccugaaac agaaugaaua uuuuguguuc     3780

cccuccgacg acuuugugcc ugccgauguc gaccucaugg augagcagaa cuaccaccug     3840

aucagcccca accucuuccg gguucagaag auugcucgga aaaauuaugu guuuaacaau     3900

caucuggaga cgaaggccgu ggacaacgau cuucugaagu ccaaaaagga acugagcaaa     3960

aucacauacc acuucuauca aaccccggag caucuccgcg gcauuaucaa gauccggauu     4020

aaucaccugg ggaaaaucau ucagaucgga gaauacuga                            4059


<210>  4196
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4196
augaacaaga aauacuaucu gggccucgac aucgggacca auagcguggg augggccguc       60

acagaugaga acuacaaucu guauaaguac gcuggcaaaa gaaugugggg gauucggcuu      120

uucgaagccg cggacacggc ugccgagcgc agaaugaagc gguccaaccg cagacggcug      180

ucucgcaaaa agcagagaau cgaucuccug caagaacuuu uuagcgagga aauuguuaag      240

aaagaccuga ccuucuuuau ccggcucaau gagucccgcc ugcacuucga ggauaagucu      300

acaggagaaa aauauccccu guuuguggac aaggaguaca ccgauauuga cuaucauaaa      360

caguacccua caauguacca ccucagaaag gaacugaucg agaacccgga gccccaugau      420

auccggcuug uguaucuggc cugccaccau auucucaaga aucgcggcca cuuccugauc      480

aacgggagca uuuccgacgu caaaaaucuu ucuuuugugu guggagaaau gaucaaggag      540

uucaacagcg uuuccgaaua cgagauuagc cugaccgaua ucagagaacu cgagcggauu      600

cugucugaca aaaagacagc uaaauccgug aaggccaaac aggucgcuca gcuguuugag      660

ugcaccccug ccgaagauaa ggagcaacag aagugugcga aagccaucau cacgaaucuc      720

agcaagcuga uugugggccu uaaaggcaac aucucuaaga uguuccacca gccccuggaa      780

gagauuucca ccaagagcuu uaaguucaca gaagcuaagu augaggacga gaucaugccu      840

gccauugaag agaauuaccc cgacuaugcu ucuguuaucg aucgcaucaa agggguguac      900

gacugguccg uccucgugga aauucuggau ggagaggacu aucuuagcuu ugccaaggug      960

aaagcguacg aaaagcaugg caaaaaccug cacauccuca agaaugucau gaagaaauac     1020

ugcaaggaug agguuuauca ggccuucuuc aacgggauua acgagaaaaa uggauacggc     1080

aacuauaucg gggcuauuag aaagaaugga cauaccuaca aaauggaccg guguaagucc     1140

gaagaggauu uuuauaaggg ccugcagaaa auccugucua caaucgugcc ugaccaggaa     1200

gaugaggagc uccuggaaaa acuuaaagcc gagguggaaa accagacccu gcucccgcug     1260

gcuagaagca aggagaaugg cacgauuccc aaccaggucc accuucaaga acugaugaaa     1320

auccucgaca augccaagga guaccuggau uuccugaaaa aggaggacga auaugggaca     1380

auugccgaga agaucauuuc caucgcuaaa uuucgcauuc cuuacuacgu gggaccccuc     1440

agccggagac acaaggaaga gggcucuaac uccuggauga uccgcaaaga agaggggcgg     1500

aucuauccuu ggaauuucgg agacaagguu gauguggagc agagcaacaa agaauuuauu     1560

gagagaauga ccaauaagug cacauaccug aucggcgaag acguccuucc caagaacucu     1620

cugcucuaua gcaaauacau ggugcugaau gagcugaaca aucuuaagau ucgcgggaac     1680

aaaaucuccg uugaacucaa gcagcggauu uauaaagauc uguucuguac cauggcgaag     1740

gugaccggaa agagaauccu ggaguaccuc aaaauggagg acccggaaau cguccaagag     1800

gauauuucug gcuuugacau cgauuucaag agcgcccugg cuuccuaucu ugacuuuaaa     1860

aagcaagugg ucggcaacga aauugagaaa gaucauuaca aggagaucau ugaagagauc     1920

aucaagugga aaacaauuua cgacgaugac agcaagauga ugcgccggau gaucgaaaga     1980

gaguauccug aaguguucga uaaagagcag auuaagaaaa ucugccagcu gaaguacucu     2040

ggguggggaa auuuuuccga ccgcuuccuc aacggcguua gaggggccga caaagagacg     2100

gaagagagcu auaccauuau cgaagcccug uggaagacaa auuacaaccu gaugcaacuu     2160

cucucuaagc aguuuuccuu caaagaggaa aucgaucaga uuaaugcuga gaaggccgga     2220

gacgugggca aaaucaacua ugagaauacc gucaaggauc ugauugugag ccccuccaac     2280

aaacgggcga ucuggcagac aauucaaauc gcugaagaga uuaagaaggu uaugaaaugu     2340

gaaccugagc ggaucuucau cgaaauggcc cgcgggggcg agaaggagaa aaagagaacc     2400

gugucucgga aaacgagacu gcaggaacuc uacgccggau gcaaggacga uacacgcaau     2460

uggaugaagg agauuggcga ccgggaagag agagaguuua acagccgcaa acuguaucuu     2520

uacuacaccc agauggggaa guguauguau accggagaug aaaucgacgu cgaugcucug     2580

augaaaggca auuccaacug ggacauugau cacaucuacc cccagucuag aauuaaggac     2640

gauagccucg acaauauggu gcuggugcgg aaagacguua acaauaagaa gcgcaacaaa     2700

auccuguccc ccaagaucag agaggauaug aaaggguauu ggacaaugcu uugccaaaag     2760

ggacucauua gcaaacggaa guacgaccgc cugaccagaa caacggauuu caccgacgag     2820

gaacugucug gcuuuaucga acggcagcuc gucgagacac gccaguccag caaggccgug     2880

gcugagcugc uuaacagacu guauaaagau ucugaagugg ucuacgugaa ggccgggcuc     2940

guuuccgacu uccggcauga uaaugaccug cugaaaagcc gcagagugaa cgauuaucac     3000

caugcgaagg acgccuuucu uaauauuguc gugggcaacg uuuaccacgu gcgguucacc     3060

ucuaauccua gaaaauggau caaggauaac cgcgacagca auuacuccau ucggagaguc     3120

uuuaacuaug acgugaaaca gggagauaag aucguuuggc acggcagcga gaauggggga     3180

cauucuaucg accaaauuaa gaaaaucaug ucccgcaacg auauucucua cacagaauau     3240

accuacugcg agaagggcca gcuguucaau gaaacgcugg cuaaaaaggg gaacgagaaa     3300

gccaucgcuc ucaagaagaa ucuggacccg gugaaauaug gaggcuacac cacacccaag     3360

accagcgccu uugcguucgu cgaguuugau gggaaaaaga aagaacggaa gaaccacauu     3420

guggagaucc cuguguaugu cguuaauauu gccaagagag aacccgacgc ugugaaaaag     3480

uaccuugagg aacgcaaagg cuacuccaac guggucauca agaaauaucc uaucaagaag     3540

aacucucuga uuaaaaugga uggauaccuc gcccggcuga gaggcgcuaa ugagaaggac     3600

aucauucuga aaaacgccgu gcagcuuauc cugaauaagg aucucuauga gauuguucgc     3660

aaaaucgaaa aguaccugga guauaaccuc caauaccagg ccgacgcuaa auucgaaggg     3720

uuuacaauga aggaucugaa uaugaucuau gaggagcuuu accugaagcu caaagaaagc     3780

auuuuccaga agcggcccgc gcagcaaaug aaaacccugg agaagggaaa agaagccuuc     3840

aucuccauug agaaccuggg cgaaaaggcu aaggugauca augagauuau cgccauguuu     3900

agaugugacg ccacgacagu cgcuaaccuu aaagugauca augggucuca gaacgccgga     3960

agcaugaagg uuaauaaaaa cacccugaca uccaagucuc ucaaacuggu gcaucagagc     4020

auuaccggcc ucuucgagac cgaagaggaa cuggucuga                            4059


<210>  4197
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4197
augaagaaaa aguacuaucu gggccucgac aucgggacca acagcguggg augggccguc       60

acagaugaga auuacaaccu gugcaaauuc aauggcaaga aaaugugggg gauuagacuu      120

uuugaagacg cuaagacggc cgcggagcgg cgcaccaaga gauccaaccg gcgcagaauc      180

gcucggacaa aacagcgcau ugaucugcuc caagaacugu gugagaaguc uaucuucgaa      240

aaagacccca ccuuuuucau uagacuuaau gagagcuccc ugcaccucga ggauaagucu      300

acaggagaaa aauauccucu guuuguugac aaggaguaca ccgauaucga cuaucaucgg      360

gaauacccga caguguauca ucugcgcaag gagcucaucg agaaccccga accucacgau      420

cccagacugg uguaccuugc cugcugucac auuaucaaaa gccggggcca uuuccugguc      480

aaugggucca uuucugacgc caaggaucuc agcuuuaucu gcgagaacau gauuaauucc      540

uucaacgcug ccagcgacua caaaaucaau auucaggaua ugaccgcucu ggaaacgauc      600

cuuaccgacc gcaagauggc caaaucugug aaggcgaaac agaucguuca gcuguuuaag      660

uguauuccuu gcgaggacaa ggaacaacag aaauguugca aggccaucau uacaaaccuc      720

uccaaacuga ucgugggacu gaagggcgau auuagcaaaa ucuucaauga gucuuuugag      780

gaaaucaagu ccuucaaguu ugcugagacc aaauaugaag acgagauucu ccccacaaug      840

gaagagaucu acccugauca cgccgagauu aucgaaaaga uuaaaagcau cuaugacugg      900

ucuauccugg augagauucu ugacggcgaa gaguaccugu ccuucgcuaa ggucaaagcc      960

uaugaaaagc acaagaaaaa ccuccaugau cugaagaucc uuauuaaaaa guacugugac     1020

gaugagaccu aucagagcuu cuuuaauggg augaacgaga aaaacaagua cuccaauuac     1080

aucggaucua uuagaaagaa cggccacacg uauaaagugg aaaagugcaa aagcuccgag     1140

gacuucuaca aggaacugcu caaacugcug agcucuguua auccggggca ggaggaugag     1200

ucccuccugg aacaaaucaa guuugagaca gaaaaccaga cccuucugcc ccuccugcgg     1260

acaaaagaga auggaaccau uccuaaccag gugcauaugc aggaacuuau caagauccug     1320

gacaaugcga gcaaguaucu cgacuuccug aacgagaaag augaguacgg caccacagcc     1380

gacaagauua ucucuauugc uaaauuuaag auccccuauu acgucgggcc ucugagcgau     1440

cgccacaaag aaaauggauc caacucuugg auggugagaa aggagaaugg ccggauuuau     1500

cccuggaacu ucgacgauaa gguggacgcc gaaaagagca auaaggaguu uauccaaaga     1560

augacgaaca aauguaccua ccucaucggc gaagaugucc ugccgaaaaa uucccuucug     1620

uacagccgcu auaugguucu caacgagcug aacaaucuga aaauucgggg gaacaagauc     1680

ucuguggagc uuaagcagca gauuuacaau gaccucuucu gcacaaaagc uagagugacc     1740

ggaaagaaca uccugaauua ucugaaaaag gaagauccug agcucgccgc cgaagaccug     1800

uccggcuuug augucgacuu caaaagcgcu cuuacaucuu accuggauuu uaagaagcaa     1860

gugguuggga acgagauuga aaaagacuau uacaaggaga ucaucgagga aauuaucaaa     1920

uggaagacca uuuauaauga ggacuccaaa augaugaagc gcaugaucgg acgggaauac     1980

cccgagacgc ucaaccagga ucagauuaaa aagaucugcc accugaagua caauggcugg     2040

gggaacuuca gcgagacauu ucugaaugga aucaaaggcg cgaacaagga aaccggcgag     2100

uguuauacca uuaucgaagc ccuuuggaaa acaaauuaca accucaugca gcugcugucc     2160

aagcaauucu cuuucaaaga ggaaauugac cagaucaaug cugagaagac cggggccauu     2220

ggaaagauca cauaugagaa ccucgugaaa gaucuggucg ugagcccugc caacaagaga     2280

gcuauuuggc agacgaucca gaucaccgaa gagauuaaaa agguuaugaa augcgaaccc     2340

gagcgcaucu uuauugaaau ggcccggggc ggggagaagg agaagaaaag aacagugucc     2400

cgcaagaccc ggcuucaaga acuguacaca ggauguaaag acgauaccag agacuggaug     2460

aaggagaucg gcgaucgcga agaacgggag uucucuagca gaaaacucua ucuguacuau     2520

acgcagaugg ggaagugcau guacaccggc gaggagauug acgucgaucu gcuuaugaca     2580

gcuaauucca agugggacau cgaucauauc uacccccaga gccggauuaa agacgauucu     2640

uuugacaaca uggugcucgu ccgcaaggac aucaauaaca augugaaagg aaacaauauu     2700

guuccuucca gcauccagaa ggaaaugacc ucuuauugga aaaugcugcu gcaaaaggag     2760

cucauuucca agaaaaagua cgaaagacug acacggagca ccgauuucac ggacgaggag     2820

cuuucuggcu uuaucgaaag acagcuggug gagacacgcc agagcuccaa agcggucgcc     2880

gcccuccuga accggcugua uaagaauagc gaaaucgugu acguuaaagc ugggcuugug     2940

ucugauuuca gacacgacaa caagcuccug aaaucccgca gaauuaauga uuaucaucac     3000

gccaaggacg cuuuucugaa caucgucgug ggaaauuguu accggaccaa guucacagcc     3060

aacccgcgcc aguggauuaa aaagaauaga gauaccaacu auagcauccg gcgcguguuu     3120

gacuucgaua uuaaacaagg cgacaagauc guuugguacg ggaacaaaaa ugagaacgga     3180

aagcauacca uugaucagau caagaaagcg auguccagaa augacauccu cuacacagaa     3240

uauacguacu gcgagaaggg caaacuguuu gacgagaccc uuacaaagaa agggaacaag     3300

aaggccauuc cccugaaaaa gaaucucgau ccucugaaau auggcggaua caccucuguc     3360

aagacaagcg cuuucgccuu uaucgaguuc gacgauaaaa agaaggaacg gaaaaaccac     3420

auuguggaaa uccccauuua uguggcuaau aucgccaagc gcgagccuga agcggucauc     3480

aaauaccugg aggagcagaa gggcuaugug aacauuguug ugaaaaagua ccccaucaaa     3540

aagaacuccc uucugaagau ugacggguac cucgccagac ugcggggagg cgauucuagc     3600

aucauuaccc ucaaaaaugc uguccaacug gugcuugaca agaaauccua ugaaaucauc     3660

cgcaagguug agaaauaccu ggaucacaau ucuaaguaug aagccaacag caagcucgag     3720

aaauucaaug aaaacgaccu gaauauuauc uacaacgagc ugugucuuaa gcugagaaau     3780

uccauuuauc agaaacggcc ggugcaucag cucgagacgc uggaacaggg gaucgauaac     3840

uuuagaagcc ucgagucucu gaaggacaaa gcuaagauua ucaacgaacu ucuggccaug     3900

uuccgcugcg augccaagac aggaauggac cuccggcuga uuggcggggc uuccaauagc     3960

ggcagaaugc gcaucucuaa aucccggcug accagcaagu cucuuaaacu gaucaaccaa     4020

uccgucaccg gacucuuuga gacagaggag gaacuggugu ga                        4062


<210>  4198
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4198
auggagaaga acuacuaucu gggccucgac auggggacca gcuccguggg augggccguc       60

acagaugaaa auuacaaccu gaugagaaag aagggcaaag accuuugggg aguucggcug      120

uucaaggagg cuaaaacguc ugccgaacgc agagugaagc ggaccagccg cagacggcuc      180

cagcgcgaga aggcgagaau cggguaucug aaagaacuuu uugcugauga gauuaauaag      240

guggacgccg gcuucuacca acggauggag gauuccaaau uuaucucuga cgauaagaca      300

guccaccaga aauucacccu guuuagcgac acaaacuaua ccgaugaaga cuacuauaau      360

gauuacccca caauuuucca ucuccgcaag aagcugaucg aguccgacga ugaauacgac      420

gugagaaugg uuuuucuggc ccuccugaac auguauaaac accgggggca uuuccuuaau      480

gcuaaccugu cugacaaugg aaugggcgau auuaacgagc aguacgaccg ccucaaggag      540

cugugcagcg aauaucuuaa uguggagugg gaaaacaaac cugauaaguu ugaggaaauc      600

cugaaagagu guaauugcuc caacagccuc agaaaugagc ggcugcugga aaccuucaac      660

cucaagaaaa cggacaagga gaagugugaa augcugaaac uuaucugcgg ccugucuggg      720

acccucucca agauuuuuaa agaucuggac gaugacagcg agacaaagaa aaugaccauc      780

ucuuuccgcu ccagcaacua cgaugaagag gccauuaaug ucgaggaaau cauuggaucu      840

gagaacuuug aaaucauugc ugugcuuaag gagauccacg acugguccau gcuggccaau      900

aucaugagag gcaagaaaua ucucagcguu gcgcgggugg aagaguacga gaagcauaaa      960

gaugaccuga agauucugaa aaaggucauc aaggaauauu ccauugagga auacaacaau     1020

uucuucagag ugauggccga caacaauuac ucugcuuaug ugaacagcgu caauuccaac     1080

aaagagagca cacgcagagg ggccaagugu gauaaugaca ccuuuuucaa agagaucaag     1140

aaaauuguua acaagugcua cgauucugaa gagaaggaau auauccucaa agagaucgaa     1200

aagggaacgu uucugccgaa acagauuauc uccagcaaug gcgugauucc caaccaagug     1260

cacaagaucg agaugaaaaa gauucuucag aaugcugaga aauaccugcc uuuccucaca     1320

gaaaaggacg auucugggcu gagcgucucc gagagaaucc uucagauguu uucuuuccag     1380

auccccuauu acauuggacc uaucagcggc aagauuggcu ccgacgagaa aagcaagaac     1440

ucuugguccg ugcgcaaaga gaaggggcgg aucuaucccu ggaacuuuaa agauaagauu     1500

gacgaaaagg agagcgccga gaaauucauc gaaagaaugg uuaaucacug uaccuaccug     1560

aacaaugaga uggugcuccc uaagaacucu cugcuguacg aaaaauuuau gguccucaau     1620

gagcugaaca aucuuaagau uaacaaugaa aacaucuccg uggagcugaa acaaagccuc     1680

uauaauaacc uguucaagac aggaaagaaa guuaccgcga agggcaucaa auccuaccug     1740

gagcgcaaug ggcuuugcga accgaacgag gauauuaauc ucaccggaau cgacggcgau     1800

uuuacaaacc ggcugucuaa cuaugccaag uucaaaggga uuuuuaauac ggacgaacug     1860

accuacgagc aguggaacau ggcugagaag aucauuaagu ggagcacaau cuauggagau     1920

uccaaaaagu uccucgaaga gaaaaucaga aaugaauacu cugagaagcu ggacagccag     1980

cagauuaaac gcauccuugg cauuaaguuc cgggacuggg gcagacuguc cgaagaacuc     2040

cugaacaugg agggggccga ugugaauacc ggagaaauca aaagcgucau uggccgcaug     2100

ugggacgaga acuauaaucu gauggaacuu aucgcuacag auaacuuuac cuacaagcaa     2160

gugaucaagg agaaacagaa ugggcucgac aagucucugu ccgaaauuga uuacgacgau     2220

cuggacgagc ucuaugucag cgcccccgug aaacggauga ucuggcagac gcugcuuauu     2280

aucaaggagc ugguuucugu gauuggauac gaaccuacaa gagucuucgu ggagauggcc     2340

aaagauguua acaccgaaaa gaagcggacc gacucccgca aaaagaaauu ucucgaucug     2400

uauaagcaau gugagaaaga cgaaagagau uucuggauca aggagaucga cgacaagagc     2460

gaggcugaau uucggaaaaa gaaacuguac cuuuauuaca cacagaaggg cagaugcaug     2520

uauaccgggg agcagauuaa uccccucgau cugacaaacg acaaucugua cgauaucgac     2580

cauauuuacc cucgccacuu cgugaaagau gacucuaucg aaaacaaucu cguccuggug     2640

aagaaggaga aaaacgcgca uaagagcgau acguuuccca uugaggacga uauccggaaa     2700

aaccaguaug ccuucuggaa gaugcuuuac gagaaaaauu uuauuaccaa ggugaaguau     2760

caaagacuga cacgcaaaga gccguucucc gacgaagaga aggcuaacuu uaucaugaga     2820

cagaucguug aaacccggca gggcacaaaa gucauuaccg auauccucga aaagagcuuc     2880

gacaaaacgc agauugugua cgugaaggcc ggaaaugucu cucuguuucg ccaaaaauau     2940

gaguguauca agugcagaga aauuaacgac uuccaccacg ccaaugaugc uuaccugaac     3000

aucgugguug gcaaugugua ugacaccaag uucacauccg auccucggaa cuuuaucaaa     3060

gaguaccgca agaaucccca gaaaaacgaa uaccauaugu auaaacuuuu cgacuacaau     3120

guccagagag gggaucgggu ggccuggacc gcgagcaacg gagaguccau gaaaguugug     3180

aagggcacac ucaagaaaaa uaccccucug gucacguuua ugucuuauga ggaacacggg     3240

ggacuguacg accagaacau uaucagcgcu aaggauauug agaaagugaa gggcaaaggg     3300

uaucucccca ucaaguccuc ugacagccgc cugaauaaca cagaaaagua cggcggauau     3360

gccaaaguuu ccggcgccua cuucuuugug gucgagcaua ccgugaagaa ugugcggauu     3420

cggacaaucg aacccaugcc uugcuaccuu accaaagucc ugaccacaaa ggagaaaauc     3480

gaagauuauu guugcaacau ucucaaguac gacucuccgg uugugaagua ugagaaaauc     3540

aagauguaca gccugauuaa agugaacggg uaucccaugu accugacggg acgcaccggc     3600

aaucaacuua ucgucuccaa cgggauucag cuggugcuca gcuaugauga ggcuaaguac     3660

aucaaagacc ugcucaagau uaaucaggaa gaguucgauc agaacagacu ggaaaaucuu     3720

ggaaucaaca aauuucggaa ugagaagcug uacgacacac ucaacaagaa auauaccaau     3780

gagaucuaca agaaaagacc uaacucuaug ggcaauaaga ugauugaaug gaaaaacaca     3840

uuccaaaauc uguccaucaa ggaccagacc gagguucugc uucagcugau ucaacucggg     3900

cagaagcaga acagcggcgu gaaucuggaa aaacucggag ccucuaagaa aacgggcguc     3960

ucccugguga gcaagaaaau cacagauaag gagucuuuug aacuuauuaa caaguccauc     4020

accgggcugu augagaacaa aguugaccuc cugcgcgugu ga                        4062


<210>  4199
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4199
auggccgcuu ucaaguacua ucugggccuc gacaugggga ccaacagcgu gggaugggcc       60

gucacagauc ccaauuacaa ccugcuuaaa gcgaagggca aagaccugug ggggaucaga      120

gaguuuaaug aagcuagcac ggccguugag cggcgcaccc acagaauuuc ucggcgcaga      180

cggcagcgcg aacaagugag aaucggacuc cugaaggauu auuuccauga cgccauuggc      240

gagguggauc cugacuuuuu ccagcggcuu gcuaacagca aauaccaccu ggaagauaag      300

gacacagagg uccgcuauaa gaauaacgug uuuaaugaug acgauuacac cgacaaagau      360

uauuucgacc aguacccgac aaucuaccau cucagaaagg agcugauuga auccaccgac      420

aaacacgaug uucggcuggu guuucucgcc cugcuuaaca uguucaagca ucgcgggcac      480

uuucucaauu cuggacuggg cgagaacagc ggcgaaaaua acaucaauaa cgcuuaucug      540

gagcuuacaa aucugcucuc cgaguucacc caguacaacc ugaacgaaac gaucgacggg      600

aaaaagauug aggauauccu gucuagacgg gacaugagcc gcaccagaaa ggccgaagga      660

cucgcggaga uucugggcgu cgauuauaaa aauaagcccu acaaagaacu uauccggggg      720

cugugcggac ucaaguuuaa cgccugugcu auuuucccug agauccaauc cgacgaggug      780

cccaaacugg auauuugccu uagcgaagcc ucuuuugacg agaaguccga ugaaaucgcu      840

aauauccugg gcgaggacua uuucgaaauu aucauggcca ugaaagauau uuacgacauc      900

gggagccucg cgggaauuau gaagggccac aacuaucugu cucaggccag aguuacaucc      960

uacgagaagc auaaagagga ccugcgccuc cugaagaaug ugauccggaa auacuguagc     1020

aaggaagauu augacaacuu cuuuaauucu gaggcugaug gcuccuacgc cagcuauguc     1080

ggguccuaca acucuaaaac caagcagaga cgcgugggaa auaagcggag cuccgaggaa     1140

cuuuauaaag cuaucaagaa aauucugaag aacgugccug ccgaagacgc cgauguccag     1200

agaauccuca ugagcauuga gaaugagaca uuccugccca aacaacuuac cgcuucuaac     1260

ggcguuaucc cuaaucaggu gcacgcgaag gaaauuaaga aaauccuguc caacgccgag     1320

cgcuaccucc cguuucugcg ggaaaaggac gagacggggc ugagcguguc ugagaaaauc     1380

augaaacucu ucagcuuuca gauucccuau uacgucggac cuacaaccga aaaauccucu     1440

aaggauggcg ggaauggaug ggugguuaga aaggaggaag gccaggugcu gcccuggaac     1500

aucgacgaga aaauugauau gaaggcuaca agcgaagccu ucaucucccg caugguccgg     1560

agaugcaccu acauuagcgg cgagccugug cuucccaaau cuucccugga guaucaaucc     1620

uuuuguguuc ucaacgaaau caauaacauu aagaucgacg gggagcgcau cucuguggaa     1680

cugaaacagg auauuuacaa ggaggucuuc cagaaaggaa agcgggugac caagaaacaa     1740

cugugcaagu aucuucauac aagaggcauc auugaagccg aggagcaggu cacggggauc     1800

gacgugacca uuaauaacgc ucucacaacc uacggaaaau uuaaggccau ccugggcgau     1860

gacaucucca aagacagcgu ucagaagaug guggaaaaua uugucuucug guguacagug     1920

uauggggauu ccaagaaauu ucugaaggac cggaucgagg aaaaauacgg cgagaagcuc     1980

accaaagaac agauuaagcg caucguugga uucaaguuua aagauugggg caaccugucu     2040

aagaauuucc uugagcugag cggggcggac guguccacgg gagagucugu cagcauuauc     2100

agagcucucu ggaacaauaa ccugaaucug auggaacuua ucaacucccg gcucuauaau     2160

uacaaagaga gacuggccga auaccaaaac acaaugauga agacccugag cgauauugag     2220

gccgaggacc ucgaugaaua uuacuucucu gcuccgguga aacgcaugau cuggcagacc     2280

auucugauca uuaaggagcu ugugaagguu cugggcugcg aaccugaccg gaucuuuguc     2340

gagaugacaa gacgccccga ugaaagaaaa augcggaccg agucccgcag aaagaaauuc     2400

gaggaacucu auaagaaagu gaaggacgag gauguggacu ggaugaaagu caucgcccac     2460

gcugaugaaa cagggagcau ucggucuaag aagauguacc uguaucugac gcagaaagga     2520

cgcuguaugu acaccggcaa gcacaucgag cuuuccgacc ucuuuaauaa caaccuguau     2580

gacauugauc auguguaccc uagacacuuc guuaaagacg auaauaucga caacaaucug     2640

gugcucgucu gcaaggaaaa gaacgcccau aagagcgaua uuuacccccu ggaggacgag     2700

auccuugcgu cucagaagaa uauguggagc gaacugcggc gcuccggguu uauuacagag     2760

gaaaaauaua acagacucau cggccggaau agcuucaccg augagcaaaa ggccggauuu     2820

aucgcucgcc agcuggugga aacaucucag ggcaccaaag ggguugccaa cauucugcag     2880

caacuucucc ccgaguccaa gaucguguac gcuaaagcca gcaaugucuc cgaguucaga     2940

aacacgcggg acauuccuaa gucuagacug aucaaugaau uucaccaugc gcacgaugcc     3000

uaucugaaca uugugguugg aaauguguac uaugucaagu ucacccagaa cccgcucaau     3060

uuuaucaaaa acgacuacga ucgcgacaag accaaaaaca auuauaaccu gagcaagaug     3120

uucgacuggg auguggagcg gaauggcgaa guggcuugga ucgcccagaa aaaggacggg     3180

gaggcuggaa ccauugccac agucaagaaa guucuuggca gaaacacacc ccugaugacg     3240

cgcuacuccu uugaggggaa gggcggacuc acaaaugaaa cccuguacuc ugccaaaaag     3300

gcuaaagagg aaggcuauau cccuuucaag agcuccgaug agaagaugaa agacgugaca     3360

aaguacgggg gauucaccuc ugugaccggc gcguacuuuu uccuggucga acacacagag     3420

aaaaagaaac ggagcagaau gauugagucc gugccccuuu aucuggccca gaagaucgaa     3480

caaaacccuc augagcucga aaaauauugu gagcagguuc uggggcucau ugauuuugac     3540

auccgcgugc ggaagaucaa gauuggaagc cugaucaaaa gaaagggcua cuucuuuuau     3600

auuacgggga aaaccggcca ccgccuuauc cugcggaaug cugucaacau gugccucaag     3660

aaagaauggg ugaaguacau uucuaagcug gagaaagccg uugauaagaa uaacguggag     3720

ggaaucauua caaaagaaaa gaaugagcug cuuuacuccg aacugaucga gaaauucaag     3780

aacagcaucu uuucuaauag acccaacccg gucggcgaca agcucgauga agccaaagag     3840

aaguucauua aucuggacau cgagaaacag ugcauugugc ucugucaaau ccugcagcuu     3900

uccagcguuc uggggucuga augcgauaug acccuccugg gagaguccgc ucauuguggc     3960

aagauucuga ucagcaaaaa gaucggggac gaagauauuu aucuuaucaa ccaguccgug     4020

acaggacugu acgagaagaa aauuaaucuc cggaccgucu ga                        4062


<210>  4200
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4200
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cuucaucaau       60

gaggcugaaa acgagauuga aguguccucu aucgucaaua caggcguuag aauugugccc      120

cuuacgaccg augaggaaag cgacuuuaag aaagggaaca caaucuccau caaugccgau      180

cggacccuga agcgcggagc gagacggggc cuccagcgcu ucaaacaaag acgggaggcu      240

cugcuugaga cauuuaaccg cauuaaguuc aucgccaccg acuuugugua cgccgaaaca      300

ggggcugcca ccacguucuc uagcuaucag cugagagcua aguccgccgu cgaggcggug      360

accaaagaag aacucguuaa ggugcugcug augcucaaua aaaagcgggg auacaaaucu      420

agccgcaagg ccaagacagc ugaggaaggc gaugccauug acggcaugaa aaucgcuaag      480

gagauuuuug aaaacaaucu gaccccuggg cagugggucu auaacucccu uagcaaagga      540

ggcaaguuca ucccggauuu uuaccacucu gaccugcaga aagagcucga aaagauugug      600

agauuccaaa aucaguuuca uuccgagcag aucaacgaua aacugcuugc cgacauccag      660

gggaaaagcc ggacacaaac cucucagcac uucuccaaga cgcugaauau uccccucgcg      720

gagaacaaag gaacacgcga agagaccaag cugcagcauu aucaguggag aaaugaagcc      780

cuggcuaacg agcucgauau gaaaauccug gccuuuauua ucacagaaau uaacaaucaa      840

aucaaccaga gcucuggcua ccuuggggcu aucuccgacc ggagcaagga gcuguauuuc      900

aaucaggaga ccguuggaca auaccaguac aaacagcucc aggaaaacgu gcacgccaag      960

cugaagaauc aagucuucua ucgccaggau uaccuugacg aguuugagaa aauuugggag     1020

acccaggcca aguuccauuc cgaacugaac gagaaacuca aggcugagau cagagacauu     1080

acaaucuuuu aucagcggaa acugaagucu caaaagcacc uggugagcuu cugcgaauuu     1140

gagaaaggcc acaaggcgau uccuaaaucc agcccccucu uccaggaauu uagaaucugg     1200

cagaaucuga acaauguggu cguuaagaac gagacgacca aagagguguu cgaacuuucu     1260

gaggaacuga agcagcucau cgccgaggaa cugcaauuua aggaguccau gacagaugug     1320

cagaugcuua ccuucuguga gcugaaaaag ggcacacaua ccgucaauuu uaaaaagauu     1380

gaagggaacc gcacgaauac aagcaucuac aaggcuuucg agaagauucu cgaaguggag     1440

ggauaugaaa ucgacuuuuc uaaaaugaag ccucuggaga uuaaggagac cguugccccc     1500

aucuucgaaa cccugaacau uaauacagug auccucgauu ucgacccuac cauccagggc     1560

aacgauuuug acaaacagcc guacuaucaa cuguggcacc uucuguacag cgccgaggau     1620

gacgauaagc ucaaagaaac acugaagacg aaauucgggu uuaaggagaa ccaucugccc     1680

uucauucuua auaucaaccu ccaggcugac uacggauccc ugucugccaa ggcgauuaaa     1740

aagauccugc cucaccucau ggauggccac auuuaugaca aggcuugccu gauggucggg     1800

uacaaucaua gcuccagccu uaccacagcc cagaacaaug accgggugcu gaaggauacc     1860

cucgaacugc ugaaaaagaa cucucuuaga aaucccguug uggagaagau ccucaaccaa     1920

augaucaaug ucauuaacgc caucaugaaa gacccugcua ugggaaagcc cgaugagauu     1980

cgcguggaac uggcccggga gcugaaagcu aauaacgaac agagaaagaa agccacaucc     2040

gacaucaaua ccgcgacgac cgagcacgag cgcauucgga agcuccugca uagcgaauuu     2100

aacaucccga gagucacacg caaugauauc auucgguaua aacuuuggcu ggagaccggc     2160

ggcaucucuc ucuacacagg gaacccuauu aaagccuccg accuguucag caagaacuau     2220

gauaucgaac acauuauccc caaauccaga cuguuugacg auucuuucag caauaagacc     2280

cuuugugagc gccagcucaa caucgacaaa uccaauaaga cggcuuacuc uuuucugcag     2340

gaaaaacuga gcgagaacga uuucgccgcu uaugagaauc gggugaagga ccucuuuaag     2400

acaggaaaaa uuaaguccua caccaaauac aagaaacugc uuauggccga uaacgaaauc     2460

ccugagggcu ucauugacag acaacugcgg gaaacacagu auaucgcgaa gaaggccaaa     2520

gagauucucc uggaaguuag ccgcaaugug accgcuacca ucgggucugu cacagacaag     2580

cugagacagg auugggagcu uguggacguu augaaagagc ucaacuggga aaaguacgau     2640

aaacugggac ugacgcauau ugaggaaggc aagaaugggg agcggcucuc caagaucaaa     2700

gacuggacca agagaaacga ucaccgccac caugccaugg acgcuaucac aguggccuuu     2760

accaaacccg ccuauauuca guaccugaau aaccuuaaug cuaagacaca aggcgaaaac     2820

aaagcgaaua gcaucauugg aaucgagaac aagcaccugu aucgggauaa gaacaauaaa     2880

cucagauuca uuucucccau gaagaacuuc cgcgaggaag ccaaaaagca gcuggagucc     2940

auccugauca gcuacaaagc uaagaauaag gucgugacca aaaacaagaa uacgaccaaa     3000

aagucuggcg ggacaaacca gaaaauucag cuuaccccua gaggacggcu gcauaaggaa     3060

acaguguaug gcaaacucca acaguacgcc accaaggagg agaagguuaa ugccagcuuu     3120

acagaagacu acauccagaa agucgcuaag aaagaguauc gcgaagcccu gcucaagaga     3180

cuggaggaaa acgauaauga cccgaaaaag gcguucacgg ggaagaacgc ucuuaauaaa     3240

acccccauuu acaucucccu gaaggauaac auuaucgugc cugagaaagu gaagacaguc     3300

uggcucgaga ccgacuauac aauccggaaa gauauuaccc ccgaccugaa gaucgacaag     3360

gugauugaug uuggaaucaa acgcauucug caaaauagac uuaacgaauu uaauggcgac     3420

ccuaagaaag ccuucgugaa ccugggggag agccccaucu ggcucaauaa ggaaaaaggc     3480

auugccauca agcgggucac gaucucugga guguccaacg cucaggcccu gcacaccaag     3540

aaagaucacc ucggcaacga gauucuggac aagaauggga acccgauccc uguugauuuu     3600

auuagcacag gaaauaacca ucacguggcg aucuaccgcg acgaaaaagg caaucuucag     3660

gaggaggucg uguccuucua ugaugcuguu auuagacgga accuggggcu cucugugauc     3720

aauaagaacc augaaaaagg augggaguuu cuguucagca ugaagcagaa ugaauuuuuc     3780

aucuuucccu ccgacggcuu caacccucac gagauugauc ugcuuaaucc caacaauuac     3840

caucugaucu cuccuaacau uuuccgcguc cagaaaauca gcaccaagaa uuauauguuu     3900

aaccaccacc ucgaaacaaa ggccguggac ggggagaugc ugaaauccaa gaaagagcuc     3960

ucuaagacca gcuaccauuu cauucaaaca cccaccaacc ugaauggcau caucaaagug     4020

agaauuaacc accuuggaaa gaucguccau cugggcgaau acuga                     4065


<210>  4201
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4201
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cuucaucaau       60

gaggcugaaa acgagauuga aguguccucu aucgucaaua caggcguuag aauugugccc      120

cuuacgaccg augaggaaag cgacuuuaag aaagggaaca caaucuccau caaugccgau      180

cggacccuga agcgcggagc gagacggggc cuccagcgcu ucaaacaaag acgggaggcu      240

cugcuugaga cauuuaaccg cauuaaguuc aucgccaccg acuuugugua cgccgaaaca      300

ggggcugcca ccacguucuc uagcuaucag cugagagcua aguccgccgu cgaggcggug      360

accaaagaag aacucguuaa ggugcugcug augcucaaua aaaagcgggg auacaaaucu      420

agccgcaagg ccaagacagc ugaggaaggc gaugccauug acggcaugaa aaucgcuaag      480

gagauuuuug aaaacaaucu gaccccuggg cagugggucu auaacucccu uagcaaagga      540

ggcaaguuca ucccggauuu uuaccacucu gaccugcaga aagagcucga aaagauugug      600

agauuccaaa aucaguuuca uuccgagcag aucaacgaua aacugcuugc cgacauccag      660

gggaaaagcc ggacacaaac cucucagcac uucuccaaga cgcugaauau uccccucgcg      720

gagaacaaag gaacacgcga agagaccaag cugcagcauu aucaguggag aaaugaagcc      780

cuggcuaacg agcucgauau gaaaauccug gccuuuauua ucacagaaau uaacaaucaa      840

aucaaccaga gcucuggcua ccuuggggcu aucuccgacc ggagcaagga gcuguauuuc      900

aaucaggaga ccguuggaca auaccaguac aaacagcucc aggaaaacgu gcacgccaag      960

cugaagaauc aagucuucua ucgccaggau uaccuugacg aguuugagaa aauuugggag     1020

acccaggcca aguuccauuc cgaacugaac gagaaacuca aggcugagau cagagacauu     1080

acaaucuuuu aucagcggaa acugaagucu caaaagcacc uggugagcuu cugcgaauuu     1140

gagaaaggcc acaaggcgau uccuaaaucc agcccccucu uccaggaauu uagaaucugg     1200

cagaaucuga acaauguggu cguuaagaac gagacgacca aagagguguu cgaacuuucu     1260

gaggaacuga agcagcucau cgccgaggaa cugcaauuua aggaguccau gacagaugug     1320

cagaugcuua ccuucuguga gcugaaaaag ggcacacaua ccgucaauuu uaaaaagauu     1380

gaagggaacc gcacgaauac augcaucuac aaggcuuucg agaagauucu cgaaguggag     1440

ggauaugaaa uggacuuuag caaaaugaag ccucuggaga ucaaggagac cguugccccc     1500

auuuucgaaa cccugaacau caauacagug auucucgauu ucgacccuuc uauccagggc     1560

aacgauuuug acaaacagcc guacuaucaa cuguggcacc uucuguacag cgccgaggau     1620

gacgauaagc ucaaagaaac ccugaagaca aaauucgggu uuaaggagaa ccaucugccc     1680

uuccuucuca auaucaaccu gcaggcugac uacggauccc ugucugccaa ggcgauuaaa     1740

aagauccucc cucaccugau ggauggccac auuuaugaca aggcuugucu uauggucggg     1800

uacaaucaua gcuccagccu gacgaccgcc cagaacaaug accgggugcu caaggauaca     1860

cuggaacugc uuaaaaagaa cucucucaga aaucccguug uggagaagau ccugaaccaa     1920

augauuaaug ucaucaacgc caucaugaaa gacccugcua ugggaaagcc cgaugagauu     1980

cgcguggaac uggcccggga gcucaaagcu aauaacgaac agagaaagaa agccaccucc     2040

gacaucaaua cagcgaccac ggagcacgag cgcauucgga agcugcuuca uagcgaauuu     2100

aacaucccga gagucacccg caaugauauu auccgguaua agcuguggcu cgagacaggc     2160

ggcaucucuc uguacaccgg gaacccuauu aaaggauccg accuguucag caaggauuau     2220

gacaucgaac acauuauccc caaauccaga cuuuuugaug acucuuucag caacaagaca     2280

cucugcgagc gccagcugaa uauugauaaa uccaacaaga ccgccuacuc uuuucugcag     2340

gaaaaacuca gcgagaauga cuucgcugcc uaugagaacc gggugaagga ucuguuuaag     2400

acgggcaaaa ucaaguccua cacaaaauac aagaaacuuc ugauggcuga caaugaaauc     2460

ccugaggggu ucauugacag acaacuccgg gaaacccagu auaucgccaa gaaggcgaaa     2520

gagauucugc uggaaguuag ccgcaacgug acagccacca ucggaucugu caccgauaaa     2580

cuuagagagg acugggagcu cguggauguu augaaagaac ugaauuggga gaaguacgac     2640

aaacugggcc ucacacauau ugaagagggg aagaacggcg aacggcuguc caagaucaaa     2700

gauuggacga agagaaauga ccaccgccac caugcuaugg augccauuac cguggcuuuu     2760

acaaaacccg ccuauaucca guaccuuaac aaucugaacg ccaagaccca gggagagaau     2820

aaagcuaaca gcaucauugg caucgagaau aagcaccucu aucgggacaa gaacaacaaa     2880

cugagauuca uuucucccau gaagaauuuc cgcgaagagg cgaaaaagca acuggaaucc     2940

auccuuauua gcuacaaagc caagaacaag gucgugacca aaaauaagaa cacaacgaaa     3000

aagucugggg gaacaaauca gaaaauccag cugaccccua gaggccggcu ccauaaggag     3060

accguguaug ggaaacugca gcaauacgcu acaaaggagg aaaagguuaa cgccagcuuu     3120

accgaggauu acauccagaa agucgccaag aaagaauauc gcgaggcucu ccugaagaga     3180

cuugaagaga augacaacga uccgaaaaag gccuucacag gaaagaaugc gcugaacaaa     3240

acgcccauuu acaucucccu caaggacaau auuaucgugc cugagaaagu gaagaccguc     3300

uggcuggaaa cagauuauac cauucggaaa gacaucacac ccgaccugaa gaucgauaag     3360

gugauugacg uuggcaucaa acgcauucuu cagaacagac ugaaugaguu uaacggggau     3420

ccuaagaagg cuuucgugaa ucucggcgaa agccccaucu ggcugaacaa agagaaagga     3480

auugccauca agcgggucac cauuucuggc guguccaaug cccaagcucu ccacacgaag     3540

aaagaccacc uggggaacga aauccuugau aagaacggaa auccgauccc uguugacuuu     3600

auuagcaccg gcaacaauca ucacguggcc aucuaccgcg augagaaagg gaaccugcag     3660

gaggaagucg uguccuucua ugacgcgguu auuagacgga aucucgagcu gucugugauc     3720

aacaagaauc augaaaaagg augggaguuu cuguucagca ugaagcagaa cgaauuuuuc     3780

auuuuucccu ccgauggcuu caauccucac gagaucgacc uucugaaccc caauaacuac     3840

caucucaucu cuccuaauau uuuccgcguc cagaaaauca gcacaaagaa cuauauguuu     3900

aaucaccacc uggagaccaa ggcuguggau ggggaaaugc ucaaauccaa gaaagagcug     3960

ucuaagacaa gcuaccauuu cauucaaacc cccacaaacc uuaacggcau cauuaaagug     4020

agaaucaauc accugggaaa gaucguccau cugggcgaau acuga                     4065


<210>  4202
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4202
auggccgacu ucgcuaagua ccaacccggc augguggccg augggagaaa aaucccucug       60

gacuggaccc ucuauuaccu gcggaagaaa gcgcuuacac aagcuauucg caaggaggaa      120

cuggccugga uccuccugaa cuuuaaucag aaaagaggau auuaccagcu ucggggcgag      180

gaagaggaag agaacagcac gaagcacgag gaauauuacg agcugaaggu cguuuccgug      240

gaagccgcug aggaggggaa aggaggcaau accugguaua acgugacacu cgaaaauggg      300

uggauuuacc gcagacaguc uaagaucgag cugaacgauu gggucggaaa agugcggccg      360

uucauuguua ccacagaaua cgagaucgac ggcgugacca ucaagaaaga uaagaauggc      420

gaagucaagc gcagcuuuag agcccccucc gagaacgacu gggggcugcg gaaaaagcgc      480

acagagucuc uccuggaaga gagcggaaaa accgugggcg cuuucauuua uaaucauauc      540

cuuuccgaac cugccgauaa gauuagaggg aacuuuaucc ggacgauuga gcgcaaauuc      600

uacaagaaag aacuggaggc gauccucaga gagcaaucua agcaccagaa ggaacugaaa      660

gacauugaua cccuuaucgc cugcaucaag gagcuguauc ccaauaacaa accucaucag      720

gacagccuca uuaagaaaga uaugacauac cugcugauca ccgaccucau cuuuuaucag      780

cggccccuga aguccaagaa aagccuuauu gcugauuguc cuuacgaaua uuacgaguac      840

guuauuaagg aaacaggaga gaauaaacgc caaucuauca agugcaucgc caaauccaac      900

ccguauuacc aggaguucag acuguggcag uuuauuauca aucuccggcu guucgacaga      960

aacaacgaca aggaagugac cagcgaguau cuuucuucca uggaagauua cacgcgccug     1020

uuuacauauc ucaaugaccg gaaggauauu aaccaggaga cccugcugaa agacuucuuu     1080

aagcaaaaga aggucaaaag aggcauggag aaggaguucc ccauccgcug gaauuacauu     1140

gaggauaagg aaaagaagua uccuuguaac gagacacggc acgaacuccu gaccgcucuu     1200

gggaaagugg gaaucgacca uagcuggcug gaugacaagg aacucuucua cagacugugg     1260

caccuucugu acucugugga guccaaagaa gaggccgaag gcgcgcuccg caagcugguc     1320

gauaaugacg auuuuguuaa cgccuuccug aaaaucaagc ccuuuaagaa agaguauggc     1380

gcuuacagcg aaaaggccau uaaaaagcuc cuggcuguga ugcggauggg gaaucuuugg     1440

aacgaggugg acaucugcga gcagaccaga gaccgcauuc agaauaucau uaaaggagau     1500

aucgacgaaa agauuaagga ucggaugggc agaacauucu cccaacuguc ugacuuucag     1560

gggcucccug aguggcuggc cuguuauguc guguacggac agcacagcga agccacggau     1620

auccagcgcu ggaccaaacc cgagaaccug auggcuuaua ucaauggcuu caagcaacau     1680

ucccuucgga acccgauugu ugaacagugc auccucgaga cacugagaac cgugcacgac     1740

auuuggaaag aggcggggca uaucgaugaa auucacaucg agcugggacg gggcaugaaa     1800

agcacagccg accagcgcgc uagaaugucu aaauccauca ugcagaauga aaacaccaau     1860

cuccggauua agagccugcu uauggagcug aagaacgcca aagaguuuga aaacgucaga     1920

ccuuacucuc ccaugcaaca ggagauccuc cgcauuuaug aagagggcgc ccugcaggaa     1980

cugacgaagg aggaugcuca guacaaugag aucagcaaaa uuucccaaau ggcccagccu     2040

ucugcgaacg aacuucucaa guaccggcug uggcuggagc agaaauauug uagccccuac     2100

acagggaagc ccaucucccu cagcaagcug uucaccucug cuuaucagau cgaacacauu     2160

aucccucaau ccagauacuu ugacgauagc uucaccaaua aagugauuug cgaggccgaa     2220

guuaaccagc uuaagucuaa uaugcuggga uaugaguuua ucaaaaagca uggcggggag     2280

auugugcgcu guacaaugau cggagacguc aaacuccugg augccaagga auacuauaac     2340

uucgugaccg agcacuacac aaauaacaga cggaaaaagg aaaagcugcu uauggaggac     2400

aucccgcagg aauuucucaa ucgccaaaug aacgacucca gauacauuag caaaaagguc     2460

auggcuaucc uguccaauau ugugcgggag gaggaugaac aggaggccaa aucuaaguuc     2520

guuaucgcuu gcagcggcgg gauuacggac cgccugaaac aggauugggg ccucaacgac     2580

guguggaacu ccaucgucua ucccagauuc gaacggcuga auaaacuuac caauacagag     2640

aacuuuggac gcugggagaa caagaaaggc aaacaggugu uccaaaccuc uguuccucug     2700

gaacuccaga aagauuuuag caagaaaaga auugaccauc ggcaccaugc cauggaugcg     2760

cuggugaucg ccugugcuuc caauaacauc auuaauuacc ugaacaauga gagcgccaag     2820

ucucaggaaa agcgcgagga ccuuagaaag aagcucugcg auaaaaaccg gaucauuaga     2880

aagcccuggg aaacauucac ccaggacgcu cacacggccc uggagaaugu cguggugucc     2940

uuuaaaaacu auauccgcgu uauuaauaag gcgaccaacu acuaugagaa guacgauaaa     3000

aaugggaaga aagaaaucgc cgagcaaaag ggacaggaca ugugggcuau ccggaaaccu     3060

augcacaagg aaacagucuu cggccaggug aaccugagac gcaagauuac caucaaacuc     3120

aaggaugccc uggagaaaau ucccgcuauc ugcgacaagg aacuucggga cuauauuaau     3180

auccuggugg ccaaacaguu uaacaagaaa caaauccucg cccauuucaa gagcauuaau     3240

uacagacuga acaugaaguc ugucgauaaa guggacguuu ggcaguuuuc caacgagaag     3300

gagccgaugg uggcuacacg caaaagcguc gauaccucuu ucgacacgaa gcggaucgcg     3360

acaauuaccg auacagggau ccagaaaauu cugcuuaauu accugaaggc caagaacaau     3420

gacccuaaca ucgcuuuuac ccccgaagga auugccgaga ugaauaaaca caucaccgaa     3480

uauaacaaug gcaagcaaca ucagccuauc cucaaaguga gaguuagcga gcccaagggg     3540

gccaaauacc aggugggcga aacaggaaac aagcgcucca aguucgucga ggcucagaaa     3600

ggcacgaauc uguauuuugc cauuuacgag gauaaggaag ggaaccggag cuauaauacc     3660

aucccucuca acgaggugau ugaaagacug aaacaaggac uuucucccgu uccggacgcg     3720

aaugagaagu ccaucccucu gaaauuccac cucagcccca acgaucuggu guacgucccc     3780

aagacagacg aggaaaauga gauuaagaug ggccagaucu auaaauucgu ggauuccucu     3840

gggaccacag cuaacuuugu gccucaccgg gucagcaauc ugaucuacaa cguugacaag     3900

aaaauugccg aaaacuucug uaaucaugag aucauucaga acgaauacgg aaucggcucc     3960

ccgcagucua agaaucagaa aagcauuacc ggggagauga ucaaggccgu gugcuggaaa     4020

cuugaggugg accgccuggg caacauccac aagauuauca aguga                     4065


<210>  4203
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4203
augaagaaaa uccugggccu cgaccugggg accacaagca uuggaugggc cuucaucaag       60

gagcccgaaa aagauguggu cggcuccgag auuguugaca ugggggugag aaucgugccu      120

cuuucuagcg augaagagaa cgacuuugcu aagggaaaua cgauuuccau caacgccgau      180

cggacccuga aacgcggcgc gagacggaau cuccagcgcu ucaagcaaag acggaacgcu      240

cugcuugaaa cauuuaagga gaaaaaucug aucccgucua acuuccagua cgccgaggac      300

gggcccagcu ccaccuuuuc uacacucaag cugcgcgaua aagccgcuaa ggagaaaauu      360

gaacuggacg auuucgucaa ggugcuccug cagaucaaua agaaaagagg auauaagagc      420

ucccggaaag ccaugagcga agaggacgag ggcaccgcua uugauucuau gggcaucgcc      480

aaggaacuuu acgagaacga ccugacaccu gggcaguggg uuuaugaagc gcucaaaaau      540

ggacgcaaga acgugcccga uuuuuacaga uccgaccugc aagaggaguu caaaaagauu      600

gucaauuauc agagcgaauu uuacccugac auccuuaaca ccucuuucgu ggaggauugg      660

augggcaagg ccuccacgcc caccaaacag uauuuuaaua agcgcggggu ucagcuggcu      720

gaaaacaaag gaaagcggga ggaaagacgg cuccaagagu acaaauggcg cgccgaagcu      780

gugaauuuca agauugaccu gagcgagauc gcccugaucc ucucucagau uaacucccag      840

aucagcaacu ccucuggcua ccugggggcg auuagcgaua gauccaagga gcuuuauuuu      900

aaaaaucuga cagucggaca guaccucuau caacagguga agaaaaaccc ucacacccgg      960

cugaagggcc agguguucua cagacaagac uaucuugaug aauuugagaa aaucuggagc     1020

guccagucuu ccuucuaccc gcagcugaau gacgaacuca agcaggaggu ucgcgaugug     1080

acaauuuucu uucaacggag acucaagagc cagaaacauc ugaucucuaa cugcgaauuu     1140

gaggagcacc auaagguggu ccccaaaagc cacccuaucu uccaggaguu ccgcauuugg     1200

cagaaucuga acaaucugac ccuuaucaag aaagacaacc ugaaugaaaa gugugaacuc     1260

gcccuggagg auaagacggc ucuugccaac gagcuggcuu uuaaaaagga acucuccgug     1320

aaagacgccc ugaagguucu gggccucaaa cccucugauu gggaguucaa uuuuacaaag     1380

auugaaggga accggaccaa ucaugccuuc uuugaggcuu ucgcgaaaau cauugaacug     1440

gaggacggag agccuaucga ccuuggcaac cugaaggccg augacauccu cgaucaauuu     1500

agcgaggcuu uccugagaau ugggaucgac acagaacugc uucaggucaa uuccgauauu     1560

gaaggagccg aguaugaaaa gcagagcuac auccaguucu ggcaccuccu guacucuucc     1620

gaggacgauc aaaaacugaa gcagaaccuc auucgcaaau uuggcuucaa gcccgagcac     1680

gccaaaaucc uggcuagcau uucucuucag gacgaucaug ccucccugag cucccgggcg     1740

aucaagaaga uccucccgca ucugcagucu gggcugauuu augacaaggc uugcaccuac     1800

gccggauaua accacagcuc cucuuuuacc aaagaugaaa augagaaaag agaacuucgc     1860

gccgagcucg aacugcugaa gaaaaacagc cuccggaauc cuguggugga gaagauccug     1920

aaccaaauga uuaauguugu gaacgcuauc cuuaaggacc ccaaucuggg cagaccugau     1980

gagauucgcg ucgaaauggc ccgggagcuc aaagcuaacg ccgaacagag aaagaauaug     2040

acauccaaca ucgcgagcgc cacgcgggac cacgagaaau accgcgagau ccugaagaaa     2100

gaguucggcc ugaagagagu gaccaagaau gaccuucucc gguauaaacu guggcuggaa     2160

acagauggga uuucucucua caccggaaag cccaucgagg cuuccaaacu guuuagcaag     2220

gaauaugaca uugaacacau cauucccaaa gccagacuuu ucgaugacuc uuuuuccaac     2280

aagacaaucu gugagcgcca gcugaauauc gauaaagcua acgucaccgc cuucagcuuu     2340

cuccaaaaua agcugucugc ggacgaguuc gaacaguacc agagccgggu gaagucccug     2400

uacggcaaac uuagcaaggc caaaauucag aagcuccuga uggcuaacga uaaaaucccu     2460

gaggacuuua uugccagaca acugcaggaa acgcgcuaua ucucuaagaa ggcuaaagag     2520

auucucuucg aaaucacaau gagaguuucc gugaccaccg ggacaaucac cgauaagcug     2580

cgggaggacu ggggacuugu cgagauuaug aaagaacuga acugggagaa guacgauaaa     2640

cucggccuga cauauacgau cgaagggaag cauggcgagc gccugaauaa gauuaaagac     2700

uggaacaaga gaaaugauca ccggcaucac gccauggacg cccuuaccgu ggcucugaca     2760

aaaccggcgu acauccagua ucucaacaau cugaacgcca aggcuauugg aaauaaaaag     2820

ggcaccgaaa ucuuugccau ugagcagaag uaccucaaac gcgagaacgg gaagcugugc     2880

uucauucccc cuaucgaaaa uaucagagcc gaggcuaaaa agcaucuuag caaaauccug     2940

guuuccuaua aggccaagaa caaagugguc acaauuaaua agaacaaaac caagaaaaag     3000

gcgggauuca augaacaaac ggcucucaca ccccggggcc agcugcacaa agagaccgug     3060

uacgggaagu cuuuucacua cagcaccaag uucgagaaaa ucggagccuc cuuugacgcc     3120

cagaagauua acacaguggc uaaaaaggag gaacgcgaag cccugcuuaa aagacuggcg     3180

gagaauggca acgauccuaa gaaggcuuuc accgggaaaa acucucucaa uaagaugccc     3240

aucuaucugg accucggcaa aaacaucaag cugagcgaaa aaguuaagac ggucgugcuu     3300

gagcagaauu acacaauucg gaagaacauc gauccggacc ugaaagugga uaaggucauu     3360

gacgugggaa ucaaacgcau ucucgagucc agacuggaag aguuuggcgg gaaugccaag     3420

cuggccuucu cuaaccuuga agagaauccu aucuggcuga acaaagaaaa gggaaucccc     3480

auuaagcggg uuaaaaucag cggcgugucc aaugucgaga gccuccaugu gaagaaagau     3540

cacuuugggg agaagauucu ggacgaagag ggaaacgaaa ucccuguuga uuucgugucu     3600

accggcaaua accaucacgu cgcuauuuau gaggacgaag augggaaucu ccaagacgag     3660

gugguuaacu uuuacgaggc cgugaucaga cgcaaucagg gcgaauccau uaucaaauau     3720

cggcacgagg aaggauggaa cuuccuguac agcaucaagc agaaugaguu uuucgucuuc     3780

cccucucaag acuuuaaccc uaacgauuuc gaccuucugg aucccaaaaa uuaugaccug     3840

auuuccccga accucuacag agcucagaag uuuagcaagg ugauguacgg caauucugcc     3900

gugcgcgauu augucuuccg gcaucaccug gagacaguuc uuaacgacgu gaaagaacug     3960

aaggaugugg cguacaaaca gcucaagucc cugcaguauc ucaaugggau caaaaagguc     4020

agacugaacc aucuuggaca aauuauccag gugggcgagu acuga                     4065


<210>  4204
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4204
augacccugc acuacaacgu gggccucgac aucgggacaa auagcgucgg auauacgauu       60

guugaugaca acggcaagau ccuucaggug aaagggaaga auggauacgg cgugagaguc      120

uucaaagagg gggccaccgc ugccgaacgg cgcauuuuua gaacaaccca ucggcgccuu      180

aagagacgga aauggcgccu gagacuccug caagauuucu uugagcccua uauccuuccu      240

caggacgaug gauucuuuau ucggcgcaag gaauccaacc uggugcucaa ugacagagau      300

gaggacacag cgucucuguu caacgaucgg agcgaccgcg auuuuuacca ggcuuauccg      360

accaucuacc accugagaca ggcccucaug acagaaaagc ggcaauucga cguucgcgag      420

aucuaucugg ccaugcauca cauuaucaaa uacagaggcc auuuucuucu gaauggcacc      480

gugaacaauu ucacgaacaa uaagauugau cuccacuccg acuuugagga acugaacgcc      540

cuucugcuca augucauccc cgagcugaaa uuccagauug accuggauca cucuaccaug      600

uuucagacaa cccuccugaa cacaaccauc agccgguccg ggagacagcg cgaccuugug      660

aagucuuucu acaauggaag cgaugacaaa caacuggcua agcagcagaa guccguugcu      720

acggaacucc ugaaagccau ucuuggccug aaggcgaaau uccauaagcu cuuuaaccug      780

gaucugcucg agacaaccga gugggaguuc agcuuugacg ccgaggauau cgacgauaaa      840

cuggcuaagc uggaaccucu gaugacagag aaccagcaac aggugaugga cauccuccag      900

cggauuuucg ccgcuaucac ccugaauggg auuguccccg aaggaaaaac ccuuucugcc      960

uccaaggugg auagcuauca aaagcacaaa caggaccuga agcuccugaa agcgaucgcc     1020

cagcauucug acaagaaaac agcuacggug cugcagaccg ccuacaacga guauauuaau     1080

ggcaucgcuu ccaagccucu caagcaagag gauuuuuaca aaagacugac aaacgccguc     1140

aagaaaagcu cugaccccca gguugccgau augcuugcuc ugaucgacca ggaaaaguuc     1200

cucccuaaac agcgcaccaa ggagaauggg gcgauuccgc accaacugca ccagcuggaa     1260

cuugagcgga ucauugauaa ccagaaggac uauuaccccu ggcucgccga acugaauccu     1320

aaccaggcua gacaaaaagu ggccaaguau aaacuguccg agcucguggc cuuucgcguc     1380

cccuacuacg ugggaccucu gaucgagccc gaaguucagc aggcuacaag caaugcccau     1440

uucgcgugga uggcucggaa ggaggccggc ccgauuaccc cuuggaacuu ugaucagaaa     1500

guggacagaa augucuccgc cgaacgcuuc aucaagcgga ugacgacaaa ggauaccuau     1560

cuucuggcug aggacgugcu cccccugcac ucucugcuuu accaaagauu uaucguucuc     1620

aacgaacuga auaacgugcg cgucaauggc cagaaacuga ccaagaaaca gaagcaagcc     1680

guguaucagg aucucuucaa acggcagccu caugucacaa agaaacaguu uaagagcuac     1740

cuggugcaaa ccggggaguu cgcugagucc agcaagauug aaggacuugc caacgagaca     1800

ucuuucaacu ccggccugac gaccgaaaau gagcucagaa aaaucuuugg ggcgcagcug     1860

gacgaggccc gguaucaggu ugauuucgaa cagauuaucg agugggcuac acuguuugaa     1920

gacgccaaga uucuucgcac caaacucgcu gagaucacau ggcugaccac ggaacaaauu     1980

gagaagcugg ccggaaucag auaccggggc ugggggagau ucagccgcaa acuccuggcg     2040

ggacuucggg accagaacgg ccagcagauc auugaucugc ucugggacac ccccaauaac     2100

uuuaugguga ucgucucuca agccgcuuuc agcgaggcca uuacaaagga aaaugagaag     2160

cugaucgaua gacgcggcgc ucaggacgug auuaccgauc uguauacauc uccccagaac     2220

aaaaaggccc uuagacaagu ucucgccauc guggcugacg uccagaaagc gaugggaggg     2280

gugccuccgc agcggaucuu uauugaguuc gcccgcgaag augaaaagaa ucccagacgg     2340

uccguggagc gcagcagaca gcuggagaaa cuguaccaaa ccaucuccaa cgaauuucuc     2400

auuaauagcg agguucggca ggaacugaag gaggcugucg accagaaggu gaacuucaaa     2460

gaucgccuuu uucuguacuu ccuccagggc gggguggacc uguauucugg aaagagaauc     2520

aauauugauc aacuguccca cuacgacauc gaucauaucc uuccucagag cuuuauuaaa     2580

gacgacucuc uggauaaccg gguccucgug ucccagaagc ugaaucgcag caaauccgac     2640

ucuguucccc ucagcgaguu cgccaacuau aaguucggcc cugccaugca ggcuaagugg     2700

cugcaacuua aagaagccgg gcugcucucc aagagaaaau acgauaaucu gacgcuggac     2760

cccacaaaac uuucuaaguu ugcgccgcug ggcuucguga accggcagcu cgucgagacc     2820

agacagguga ucaagcuggc ugccaaccuc cuggccagcg aagagacaaa aguugugacc     2880

auuaaggcua auaugaccca ccagguccgc aaggaacuug auuuuccuaa aaaccggaau     2940

gugaacaauu aucaccaugc cuucgacgcg uaccugacag cuuuuguugg aaucuuccuc     3000

uccaagagau aucccaaacu gaagccuuac uuuacguacg gcgauuucca aaaagggaac     3060

aagcugcccg accuuaagaa uuuuaacuuc cuguaugagc ucaaaaauaa ggaucgcagc     3120

auugacucua acaccggaga gaucaucugg gauaaacagc gggaccuggc cuacaugaau     3180

aagauuuaua acuuuaaaaa ggugacaguc gugcacgaag ugcucaccaa guccggcgcc     3240

cuguacaauc agacacuuua uaaagcuagc gaggacaagg ccucugggag aggaaccaaa     3300

caacugaucc gcaagaaaga uaacaugccu acggaacugu acggcgggua uacaggcucc     3360

accagcgcuu ucaugucuau uguccggcuc uggaagaagg acaaacccua cuacaagguu     3420

gugggaaucc cgaccagaau ggccgcgaaa cuggccaauc aggcucagcu ucuggauuau     3480

cucacaaaga aauucaccac acgcaagcug gugaagaaaa cgggcgacua caagaccaca     3540

gucgagcggu uugaacucgu gguuccuaaa gugggguuca accagcuggu cauugaugga     3600

ggccaacccu uuaugcuugg gagcgccacc uaucaguaca acgcuagaga gcuguucauc     3660

uccaaggagg ccgugaaagc ccugaauaag cagcucagca cacauguuga ccuggugcag     3720

gucuuugaug aaauucuugu gcaaguuaac cggugguucc cccuguauga caccaaugga     3780

uuucgcgaga aacucucuca cggcagagau cgguucauga agcugaacga cuccuuugaa     3840

gaaaagagcg auacgcagau cgacgugcuc aaucgcaucc ugauuggguu ccacgcuaac     3900

gcggccagaa ccaaucuuaa aauccugggc cugggaacag aucucggcuu uaugacccag     3960

caggcuggga uucggcugac agaaaaggcc auccuugucc aucaaucccc uucuggacug     4020

uucgagagaa aaguggcccu ccgcgaccug aagggccucc gguga                     4065


<210>  4205
<211>  4068
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4205
augaccuaca gcaucggccu ggacauuggg acaggauccg ugggcugggc cgucaucggg       60

gaugacuaua gacucaagcg ggcuaaagga aagaaccuga uuggcguucg ccuuuucgau      120

ucugccacga ccgcggcuga gagacggggg uaccgcacaa ccagacggcg ccugagcaga      180

cggcacuggc gccucagacu gcuuaaugac aucuuugccu ccgaucuggc cgaacaggac      240

gagaacuucc ucccccggcu gaaauauucu ugggugaauc cugaugacga acaaaacccg      300

cagcugaaua gcgauguggc uaacggagcc cucuuuggcu ccucugagac agacaaggcu      360

uuccauaaaa auuaccccac cauuuaucac cuucgccaug aacuuaugac agauaccgcc      420

aagcacgacc ugagagaggu cuaccucgcg auccaucaca ucgugaagua ucggggcaac      480

uuucugaaua cggccgauaa aauuaacgcu gagaauagcu ucaacguuga ccuuuuuauc      540

gccgcucugg acgaauacaa ugaguuccuc gaaaccccua uuacauccau caaggaugcc      600

cagcuguuua gcgacaaacu gaccgauauu aacgcgcgca auaagucugu gagagucgag      660

aacgccaugu ccgagacaua cgacgauaaa agcaagaucu ucaaggcuau ucucaccgcc      720

cuggugggga acacggcuaa uuuucagaca aucuucgacc uuucugaucu ggaaaaagac      780

gaggccaaga acuuuaaauu caagcucgau cuggaagacg uugagaccaa acuugaugug      840

guccgggcca agcuguccga cgaacaaaau gcuuuccucg aggcggugcu gaucgccuau      900

gacggaauua cacugaaaau gauccucggc gauuacaaga gcauuucuca guccaugauc      960

gagagcuaua agucccacca gagagacugg aaauacauua agaacaaugu gcgccugaac     1020

gaacagaaaa ucgagcccga uaaaaauucu gucaaauuuu ucaagcugga cccuaagaaa     1080

cugaucuauc ccagcaaccu cgcuuaccug caaguuauuu ccagcaauga agaugagcau     1140

cggaaggggc uugacuauuu uaaaaccgcc auccaggaug ccgacauucu gcagagaauc     1200

gaaaacgaua ccuuccuccc ucgccagcgg acaaagagaa auggagugcu gccguaccaa     1260

cugcaccucg cugagcugga gcagauuauc aaaaagcagu cuaaguacua ucccuuucuu     1320

gccaacaucu acacgcaaga caauaaaacc gaaaacaagc ugauuggccu ccugaaauuc     1380

cgcgugccuu auuacgucgg gccccuggug gaggcggaaa agguuacagg agauggcaaa     1440

aaucauuggc uuaucaagaa ggagcuccag gaaaacaccc cuauuacacc cuuuaacuuc     1500

gacgaugugg ucgacaaaga caaguccggc gagcaguuua ucaaacggau uaccgggacg     1560

gauagcuauc ugaucggaga gccgacacug ccugaaaauu cucuccugua ccagaaguau     1620

aacgugcuuc aagagcugaa uaacguuaga aaugaacgcc accagcggcu ccccauuaaa     1680

gugaagcagg acaucuucga gcacgucuuu aaaaucgaua agaacgugaa ugcuaagaaa     1740

gucauggccu accugaagcu gaacggcuac ggggacuucc agauugccgg acuuagcgau     1800

accagaaccg gcaaauuuaa uuccaaccuc ucuagcuaua auuacuucgu ggaccugcug     1860

gggcgcgaga agacagaaaa gauccgggag gauagacucg aagagauuau cgaaauucaa     1920

accguuuucg aggacaagau ccugguggcu aagcggcuuu ccaaccugga uuuucucaca     1980

ccucaggaga aaagcaagcu gucugccaaa cauuauacgg gauggggccg ccugucccag     2040

aaacuucuca ccacacaguu caucaagacc gcucugagcg aagccggcga cauuaucccc     2100

caaucucacu ccauuaucga ucugcucuac uauacagaca agaaucugau ggagauucuu     2160

aacgauaaga ccuuuaaugu cagaaacugg cugacggaac agaacaaagg gaccgagagc     2220

ggaaauuccc ucuacgacca gaucgacgcg cuggccggcc ccaaggauau caaacggggg     2280

auuacacagg uguucucuau cauugacgau aucaagaaag cuaugggaag caacccugaa     2340

accguuuauc uggaguuugc cagagagaca caaaccuccc gccuuacaaa uucucggcuc     2400

aacagagugc aggaaauuua caauagcccg gagcugaaga cggaguucgc uuaccuggcc     2460

aagaaacucg aagcggaaac caaggagucc auccaugacg aucgccugua ucuuuacuau     2520

cugcaggagg gcagagacau guacacaggg aaagaaaucc ccuuugauca aauuagcucu     2580

aacuaugaca ucgaucacau uaucccucag gccuucacca aggacgauuc ccucgacaau     2640

cggguccugg ugaaccgcgc uaccaaugcc agaaaaagca acucucccac auuuacgaau     2700

gacauuaucg auaaccggaa guccuggugg cagagccugg ugaaacgcgg ccuuaugucu     2760

aaggagaagu ucgaaagacu gaccgacaaa cggaagacau uuagcgagaa ucagaccaac     2820

cauuucauug cucgccaacu cguugagaca agacagauca ucaaaaaugu cgccgaucug     2880

auuaagaaca acuacgacgg aacccaggcc guggcuaucc gggcgucccu cacgggcgaa     2940

augcgcagau aucuggauug gccuaaaaau cgggacauga acgauuacca ccacgcccau     3000

gacgcucuua ugauggccac aguggggaag uacgucgaua gacgcggauu uuucgacaau     3060

ggcagcgugu cugauggggc cggaaacgcu uauaaucugu acaccaagga cuggcucguu     3120

gccgcgcggg ugggcaccag aaacgauucc cagcgcguca aucccuaugg guucauugug     3180

ggcagcauga acuccaaagc uguucggcaa caggugaaua aggagacagg agaaaucguc     3240

uggggcaacg agaaucugga cuaccugaga cacgugcuuu cuuauaaaaa gauucugguu     3300

acccgccggc ucagcggguc cucuggaaga cuguacgccg aaacacucuu uagcccgaac     3360

ccuaaagcca agcugaucgc ucagaaggcc aaucgcuccg uggcgcuuua uggcggguuc     3420

acgaaagcug agucugccua caccgcccug gucgaguuua acaagaaagg agaccggagc     3480

uacaagcuca ucaaagugcc cauggcuauu gccaauagag ugaacgauaa gcagaucaca     3540

gucaccgcuu auauugccuc ccugaaucug cgggacuuca gccgcguugu gcuugaacau     3600

cugccucucg gcacacugau cagagagucu gauggguccg aauuuuacgc ggccagcucu     3660

gaguauaagc acaacgcuaa agaacucugg gcccccaagg agauucugcg guccagcaaa     3720

aagcuggaca aagcuaccgc cgauaaccug aucgacaucu ucgauuuucu gacgaaugac     3780

aagaccauuu cuaacuucaa guuuuacgag aaagaucucc ugcaccuuaa gaaugugcgc     3840

gacaaauucg ucgaagccuc cgaugagauc aagauuaaag ugcugagcga cguuaucuau     3900

gaacuccaua acgaugcugg cuggagagac ccuuuuaagc acauucaaga gaaacccgcg     3960

uggacaaugc uucaggugaa gggaggcauc aagcucuccg aaaaagccgc uauugucuac     4020

cagagcccga ccgggcuguu cgagacaauc accaucgugg gagaguga                  4068


<210>  4206
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4206
augaagaaau ucgaggaaua cuauaucggc cuggacaugg ggaccggaag cgugggcugg       60

gccgucacag auuccgcuua caacauuauc aagagacacg ggaaagcccu cuggggaauu      120

agacuguuug agucugcgaa gacggcugag gagcgcagaa ucuuccggac cagccgcaga      180

cggacagaac gcagaaagaa ucggcuucau cugcuccagg agauuuuugc cgaggaaauc      240

uccaagaagg acaugggcuu cuaucaacgc augaaagagu cuaaguacug gcacgaagau      300

aaaaaggaca ucaacgggaa uaugcccgag cugccuuaug cccuuuuugc ugaugacgga      360

uucaccgaua aagacuacua uaagaaguac ccgacaauuu uucaucugag auacgcccuc      420

gcuaccaaca caaccgauaa acccgacauc cggcugcugu aucucgcccu gcaccauauu      480

aucaagcacc gcggccacuu ccuuuuugag ggcaaaaagc ugagcgaagu uaaagaguuc      540

uccacggcga uugaagcccu cgugcagcag gcugaggaac aggagaucga ucugaagucu      600

cuuacccaag aggaagugcu gacacagcuc gagcauauua ugagcgacgc ccagugcacc      660

aaauccgaua agaagacaca ggucagcaaa cugaucgugg ggaccgacaa gcaaaaacag      720

aagcaggaaa aagcuaucgc cgcgcugauu acgggaugua caguuaagcu cucugacauc      780

uuugccgauc cugagcugga caaggaagag auuuccaaaa ucugcuucag cgauaauggc      840

uacgaggaaa aggaggugga aauugagucu guccuugcug acagauuucu gcucaucgcc      900

gcugccaaag ggguguauga uuggaccguu cugcacgaca uccuuggaga ugcccgguuc      960

cuguccgaag cuaaggugaa aacauacgag aagcaucgcg acgaucucag aaaggucaaa     1020

cagcugcugc ggauggacaa ggagguguau gaagcggugu ucggcgucag cgagaaaaac     1080

gaagccaauu acucugcuua uauugggaug gccaagaaaa acggaaagaa gcaacccauc     1140

gagaaaaagu guucccagga ggauuuuuac aaauuccuca agaaacagcu ggacagacuu     1200

agcguugacg aaaugggcca agagauuauc gaugaacuga aggccgagau ggaccucggc     1260

acccuguuuc cuaaacagau gauucgcgau aaugggguga uccccuacca gcuucacgaa     1320

aacgagcuga augcuauccu cgacaacgcc cagaaguucu auccuuuucu gcuggagaag     1380

gaucaagacg gauacacccc gguggagaaa auucagaaga ucuuccgguu ucggauuccc     1440

uauuacgucg gcccucucaa uacagcgcac caggauaaag ggggacauuc cuggucugug     1500

cgcaagaaag aaggcaagau cuaucccugg aacuucagcg aaaugauuga cgaggaagag     1560

uccgcuacga aguuuaucga gcggaugacc aauaaaugca cauaccugau uggggaagau     1620

guucuuccua aggagagccu gcucuaugcc aaauucaacg ugcugaacga acugaauaac     1680

cuuagaaucg acggagagcu caucgccccc gaacugaagc aggauauuua caauaacguc     1740

uuuaaaaagu accaacgcgu gaccggcaag aaaguuaagg acuaucugcg gaucaauggc     1800

gugauuucua aagagcagga gcucuccggg uucgaucaga acuuuaagag cucucugaag     1860

gcuuaccacg acuucaagca gaucgucgga acaggcgugc uuagcgaauc cgagcaagaa     1920

gacauuguca gagauaucac ccuguucgcc gacucucaga agaugcucaa acagcgcauc     1980

caucggaagu auccgaugcu gagcgauaaa cagauugcuc aacuggccaa uaagaaauac     2040

gcgggguggg gaagacuuuc ccgcacguuu cucgagaaca ucgagagcgu gaauaaggaa     2100

acaggcgagg uuaugaacau uaucaccgcc cugugggaaa ccaaugagaa ccugaugaaa     2160

cuccugucug ggaaguauga cuacacaaag cagauugaac aguggaaugc ucggcaaaug     2220

aaaaagggcg agaucgagua ucaggauauc gaggaacuuu acgugucccc ugccgucaaa     2280

agacccauuu ggcagaccau caagauugug cgggaaauca caaacauuau gggaggcgcu     2340

ccuaaacgca ucuucguuga gguggccaga gaacccgggg agaagggagu ccggaaggug     2400

agcagacgca aucagcugcu cgaccuguac aaagcgugua agaaagaggc ccccgaacug     2460

uaugaucggc uugcuaacaa ggagucugac aacgcccuca gauccgauaa acuguuucug     2520

uacuauagcc aaaugggccg cugcauguac uccggggacg cuaucgaucu cgacgaacug     2580

uucacgaaua gauaugauau ugaccacauc uacccucagu cuaaggugau ggacgauagc     2640

auugacaacc ggguucuugu caagcgcgau cucaauucca aaaagaccga cacauuuccg     2700

gugucugagg aauauagaaa agccggaaag gcccugugga gcacccugcu gaaaaagggc     2760

cuuaucucca aggagaaaua caagcggcuc acacgcaccg agcccuucgg ggaagaugag     2820

cuggcuggcu uuauugcgag acagcuggug gaaacgcggc agagcaccaa agccgucgcu     2880

caucuccugg aggaggccua cccugacgcc gaaaucgugu augcuaaggc ccgcgcgguu     2940

ucugaguuca gacacaaauu ugauaugauu aaggugcggg aagucaacga cuuccaccau     3000

gcuaaggaug ccuaccuuaa uaucgugguu ggaaacacau auuacgugaa auuuaccaag     3060

gacgccgcuu gguucguccg caauaacccc ggcagaacau auaaucugaa aaagauguuu     3120

gagaacgaaa ccgugaaacg ggggaaugag acggccugga ucccuggaga uaacggcaca     3180

auuuguaccg uuaagaaaug gaugagaaag aauaacgucc ucuucacacg ccgggcguac     3240

gaggugaaag ggggacuguu cgaccaaaau auccugaaaa agggcaaagg gcagauuccc     3300

aucaagggcg gagcugacga aagacuucgc ucccgggaua aauauggcgc cuacaacaag     3360

gccagcgggg cuuacuuuau gcugguggag ucugacggaa agaaaggcga aagaaagcgc     3420

accauugagu ucgugccggu cuaucucgcc aaaucccugg aagagagcgu ugagcuccgg     3480

agacgcuacu gcgaagagga acuggggcuu gugaacccug augugcggcu gucuaagauc     3540

cggaaaaaug cucucaucga cgucgaugga uuuaagaugc accugguggg cagaaccgag     3600

aaccgccugc uucugauugg ggccacacag cucguucuga gcgaagacga ucagcaacuc     3660

aucaagaaaa uuggcaagua uugucuggac cauagagaga auaaagaugc ggugcuuucc     3720

gagaaguacg aacugacgau ggaccucuuc gagaaacugu augauaucuu ugucuucaag     3780

cuggaacaga ccguguacgc cguuaaacuu agcaaacagg cuaaggaccu gaaagaggug     3840

aaggaggaau uucucgcccu ggaugacgag caaaaaugcc uccugcuuau ggaaauucug     3900

caccuguucc aguguucucc ccagcucgcu aaccugacaa auaucggagg cgggaagucc     3960

gccggaagcc uuaaaauccu guccgacauu accaagcagc aaucuguccu ccugaucaac     4020

cagagcauua caggcuuuua ugaacaggug aucgaucuca agaccauuug a              4071


<210>  4207
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4207
auggaggugg gcaacuacua ucuggggauc gacgccggaa ccgauagcgu cggcugggcu       60

guuacagaca aucaguacaa cgugauuaga aagaaaggga agucccucug gggaauccgg      120

cuguucgaug gcgccaauac ggcgcaagaa cgcagaaccu uucgggcccu ucgcagacgg      180

aacgagcgca aaaagcagag aauuaaucug cuccaggaac uguucugcaa agagaucugu      240

aaacuugacc ccaacuuuuu cauucggcug cgcgauucua gccucugguc cgaagacaag      300

gaggagaaac agaucuauuc ucuguuuaau gauaagaacu ucaaugacag aaacuaccac      360

aauaacuaua aaacaaucua ccaucugcgg gcugaucuca uuaagaauaa agaaaagcac      420

gacgcccgcc ugguguaucu ugcuauccau cacauuauga agaacagagg gcauuuucug      480

uucaauggaa acaucgagag cgucaccucc uuugaaaaca cauucaauga guuuaaaaac      540

ugccuccugg augagcuuga aauuaaucug gaguguccuu cuaucagcga guucgagaac      600

auucucaagg accggaccuu uaaaggcaca caaaagugca aaaagcugaa agaacugugu      660

uccaucccga gcucugauaa gaagcucgac aauaucuuca aacugauuuc cggccuuaag      720

gccgaccuga gcaucauuuu cgaggaugag ucuuuuaaag aaaacgagca caauaagauc      780

uccuucgcug acagcaacua cgaagagacc cgcgaagugc ucaauucuga gauucaggag      840

aaaacgcuga ucaucgauau uuuuaacucc aucuacaauu ggguuauucu uagcgacauc      900

cuguccgggg gagaauauaa cggcgggcuc uaccugucua uugccaaggu gaauaccuau      960

aacaagcaca gcaaagaucu gaagauccuc aaauccguca ucaagaaaua cuucccccag     1020

aaguauacag acauuuuugc gagcaauaag gagaacaacu acugcucuua uaucggaaug     1080

uacaaauccg gcgggaagaa auacagcgug aagaaauguu cugauaagcc ugaagaguuc     1140

uauaagacca uuaaaaauau cauuaacaag aauacagccg aagagaucag caacgacaaa     1200

gauguucagu acauucuguc cgagaucgaa aauaacucuu uucuuccccu gcaaaucaau     1260

aagaacaaug gagugauucc uuaucaggcu aacaaaaucg agcucgaaag cauucugaag     1320

aaugccgaga acuaccuucc cuuccugaaa gagaucgaca ccgaaugcaa uaagacgaca     1380

uccgagaaga uuaucgaucu cuuugaguuc agaaucccuu auuacgucgg cccgcugaac     1440

accagcaaag gcgaaaaugc uuggaugauu cggaaggaag acgggacaau cagacccugg     1500

aacuuugagc agaaagugga cauugauaag accgccgcgg aguucaucac ccgcaugaca     1560

aaccagugua cguaucugaa uaacgaaacc gugcucccua aauacucucu gcuuuacucc     1620

gaguauacag uccugaauga aauuaacaau auccggauca acggagagaa gauuagcguu     1680

gaacucaagc aagacaucuu uaauauucug uucaaaaaca auaaacaggu gaccugcaag     1740

aagcugcuuu cucaucucaa cuccaauggc uacaaccuga aaaaggauga ccugagcggg     1800

uucgauggaa auuuuaaguc cucucucagc uccuauaucg accugaaaaa cuacauuuuc     1860

ggcgauaaua ucgacaagcc ccagaucuau aaccuugccg agaaaauuau ccuguggauu     1920

acacucuacg gggaugacuc uaagcugcug aaaagagugc uucaaaagaa cuguagcgag     1980

cagcucaauc cugaacagau caagaaaauu accaagcuga aauuuaaggg auggggccgc     2040

cuguccaagg cuuuccucag cgagcugcgg ggcgccgaua gagaaacggg ggagacaaug     2100

accaucauua agggacuucg caacacccag aauaaccuga ugcaacuccu gucucagaau     2160

uauacauuua ccgaagagug cgagaaaauc aacuccggcu acuucaucaa ucccaacgaa     2220

auuagcuacg acucuaucgu caagaauauu guggcuuccc cgagcaucaa gcggucuguu     2280

uggcagacaa uucagaucgu ggaggaaauc caaaaaguca uggggagcaa gccuagaaaa     2340

auuuuugugg agauggcccg cggagaggaa gagaagguuc ggacgaccuc cagaaaacag     2400

aagcugcuug aucucuauaa gaacaucagc gacgaaugcg ccaaaggcau guacgacgag     2460

auuuauucua aggaugaauc cgacuucaaa aauauccgcc uguaccugua uuacacacag     2520

augggguguu gcauguauag cggcaagcac auugaucucu cccagcuguc uaacaccaau     2580

aucugggacc gggaucauau cuacccccaa agcaaaacaa aggacgauuc ccuugacaac     2640

cuggugcucg ucuacaaguc uauuaauagc gagaaauccg augacaucau uucuccuacc     2700

auccagaaca auaugagaac guuuuggaag cugcuguucg aucggaaauu uauuagcgag     2760

aagaaauauc accgccuuac cagaacaaca ccccucaccg acgaagaacu ggcuggauuc     2820

aucaaccggc agcuggugga aaccagacag uccacgaagg ucauggcgag cauuuuuaag     2880

cgcaucuacc ccaacacaga gaucauuuau gugaaagcca agaauguuuc ugauuuccgg     2940

cacagaaaag acugugaaaa cggcgugaag guccgcgagc ucaaugacua ccaucacgcu     3000

aaagaugccu aucugaacau cgugguuggg aauguguacu ccagcaaguu caccaacaau     3060

ccucuuaugu ggcugaagaa caauagaaac gucaaauauu cucucaaucg gauguacgac     3120

uuuaaccuga cagugaauaa cgagaauauu ugggugguug gaaacgaagg cacccaaaag     3180

accgucaaca aauaccuggg gaagaaugau auccuuuaua cacgcuacgc caugacgaac     3240

aaaggagagc uguucgacca gaauccgcuc aaggcuaccg gcgauaaauc caagcagaca     3300

acccuggugc cccucaagaa agggaaggaa guguggaaau auggcggaua caacagcauu     3360

acaccuucuu auuuuaugcu ggucgagucc aaggacaaaa agggcaauua caucagaacc     3420

aucgaaacga ugccccuuua ucgggagaag gaguucagca aaaacgaaga guuucugcuc     3480

aauuacugca aggaagugca uaaacugaag gagccuaaaa uuauccugaa guguauuaag     3540

aaaaacgccc uucugaucgu ugauggguuc cccaugcacc ucaagggauc cacaggccug     3600

caacucaaac ugcagggggc ggugcagcuu auucuggaga gcgacauggu caucuaccuc     3660

aagaaacuga ugaaguuugu gaaugaaaac aauaucaaca agaauaaacu ggagguuacc     3720

ccguauaugg aacuuucugc uaaggagaac auuaaacugu acgauaucuu cauucagaag     3780

cucaccacag gaaucuauag caaacgcccu gccaaucccg uggaaaagcu gauuaacgcc     3840

agagacaucu uuauugagcu caccgucaaa gagcaaugca ucgugcuguc cgaaauccuu     3900

caucuguucc aguguaagcc ucugacaucu gcuaaccucg uuagcauuaa ucugaagaaa     3960

acggugggca ucauucagau cgccaagaac auuaccgcua aaaaugucaa acuugugcac     4020

caguccccca cagggaucua cgaguauacc guggaucuga acaagaucug a              4071


<210>  4208
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4208
augaccaaga aagagcagcc cuacaacauc ggccuggaca uugggacaag cuccguggga       60

ugggccguca cgaaugauaa cuaugaccuc cugaauauca agaaaaagaa ccuuuggggc      120

guuagacugu ucgaagaggc ucaaaccgcc aaagaaacac ggcucaaucg cucuaccaga      180

cggcgcuaca gacggcgcaa gaacagaauu aauuggcuga acgagaucuu uagcgaggaa      240

cuugcgaaga cagauccuuc cuuccugauu cggcuccaaa auucuugggu gagcaaaaag      300

gacccggauc gcaaaagaga caaguauaac cuguuuaucg augggcccua caccgacaaa      360

gaguauuacc gggaguuccc uacaaucuuu caucugcgca aggaacucau ucugaauaag      420

gauaaagcug acaucagacu uauuuaucug gcccuccaca acauccugaa guaccgggga      480

aauuucaccu acgaacacca gaaauuuaac auuuccaauc uuaacaacaa ucugucuaag      540

gagcucaucg agcugaacca gcagcugauu aaauaugaua ucagcuuccc cgacgauugc      600

gacuggaauc auaucuccga cauucucauc ggccgcggga acgccacgca aaagagcucu      660

aauauucuga aagauuuuac ccuugacaag gaaacaaaga aacugcucaa ggaggugauc      720

aaccugauuc uuggaaaugu cgcucaccug aacaccaucu ucaaaacauc ccucaccaag      780

gaugaagaga aacugaauuu uagcggcaag gacaucgaau cuaagcugga ugaccucgau      840

uccauucugg acgaugacca guucacggug cuugaugccg cuaacagaau cuacagcaca      900

auuacccuga augagauccu caacggcgag ucuuauuuuu ccauggccaa aguuaaucag      960

uacgaaaacc augcgauuga ccuguguaaa cuucgggaca uguggcacac aaccaaaaau     1020

gaggaagccg uggagcagag cagacaggcu uaugaugacu acaucaacaa gccuaaauau     1080

gggaccaagg aacuguacac aucccucaag aaauuccuga aggucgcccu gcccacgaac     1140

cucgcuaaag aggccgagga aaagaucucu aaaggaaccu accuggugaa gccucgcaau     1200

agcgagaacg gcguugugcc guaucagcuu aauaagauug aaauggagaa aaucauugau     1260

aaccaguccc aauacuaucc cuuccugaag gaaaauaaag agaagcuccu gagcauccug     1320

ucuuuucgga uuccuuacua ugucgggccc cuucaguccg cggagaaaaa cccuuucgcc     1380

uggauggaaa gaaagagcaa uggacacgcu cgccccugga acuuugacga gaucguggau     1440

cgggaaaaau cuagcaauaa guucauuaga cgcaugacag ugaccgacuc cuaccucguc     1500

ggcgagccgg uucugccuaa gaacucucug aucuaucagc gguacgaggu gcucaaugaa     1560

cugaacaaua ucagaauuac agagaaccuu aaaaccaauc ccaucgggag ccgccugacg     1620

guggaaacaa agcaacggau uuacaacgag cucuuuaaaa aguauaaaaa ggucaccgug     1680

aagaaacuga ccaaguggcu gaucgcccag ggauacuaua aaaacccuau ucuuaucggc     1740

cucucccaga aggaugaguu caauagcaca cugaccacau accuggacau gaaaaagauc     1800

uuugggucuu ccuucaugga agauaacaag aauuaugacc agauugagga acucaucgag     1860

uggcugacga uuuuugaaga uaaacaaauc cuuaacgaga agcugcauag cucuaaauac     1920

uccuauaccc ccgaccagau uaagaaaauc agcaauauga gauacaaggg cuggggacgc     1980

cucuccaaga aaauccugau ggauauuaca accgaaacaa acacgcccca gcugcuucag     2040

cucucuaauu acagcauccu ggaccugaug ugggcuacca acaauaacuu cauuuccauc     2100

augucuaaug acaaguauga uuuuaaaaac uacauugaga aucacaaccu caauaagaac     2160

gaggaccaaa acaucagcga ucugguuaau gacauccaug uguccccugc ccuuaaacgg     2220

ggcauuaccc agagcaucaa gauuguccag gaaaucguga aauucauggg gcacgccccg     2280

aagcacauuu uuaucgaggu uacaagagaa accaagaaau cugagauuac aaccucccgg     2340

gaaaagcgca ucaaaagacu gcagagcaag cuccugaaca aagcuaauga uuucaagccc     2400

caacugcggg aguaucuugu gccuaacaag aaaauccagg aggaacucaa gaaacauaag     2460

aaugaccugu cuuccgagag aauuaugcug uacuuccucc agaacggaaa aagccuguau     2520

ucugaagaga gccuuaauau caacaagcug uccgauuacc aggucgacca cauucucccc     2580

cgcacguaua ucccugauga cagccuggag aauaaggcgc uggugcuugc caaagaaaac     2640

caacggaagg cugaugaccu gcuccucaau ucuaacguca uugauagaaa ucuggagcgc     2700

uggacauaca ugcugaacaa uaacaugauc ggccuuaaaa aguuuaaaaa ucugaccaga     2760

cgggugauca cagacaagga caagcucggg uucauucauc gccagcuggu ucagaccucc     2820

caaaugguga aaggagucgc caacauccug gauaacaugu acaagaauca gggcaccaca     2880

ugcauucagg ccagagcuaa ccuuagcacg gccuuucgga aagcgcuguc cggccaggac     2940

gauaccuauc acuucaagca ucccgaacuc gugaaaaauc gcaacguuaa ugacuuucac     3000

cacgcucaag augccuaccu ggccucuuuc cucgggacau auagacugcg gcgcuuuccg     3060

accaacgaga ugcuucugau gaauggagaa uacaacaagu ucuauggcca ggugaaggag     3120

cucuacagca aaaagaaaaa gcugccugac uccagaaaga auggguuuau cauuucuccc     3180

cuggucaacg gaacaaccca guaugaucgg aauacgggcg agaucaucug gaacgugggg     3240

uuccgcgaca agauucuuaa aaucuuuaau uaccaucagu guaacgugac aagaaagacc     3300

gaaauuaaga ccggccaauu cuacgaucag acaaucuaua gcccuaaaaa ucccaaguac     3360

aaaaagcuga uugcucagaa aaaggacaug gauccuaaca ucuauggagg cuuuuccggg     3420

gacaacaagu cuagcauuac caucguuaaa aucgacaaua acaagauuaa acccgucgcc     3480

aucccgauuc ggcucaucaa ugaucugaag gacaaaaaga cacuccagaa cuggcuggag     3540

gaaaauguga agcacaaaaa guccauucaa aucaucaaaa acaaugugcc uauuggacag     3600

aucauuuaca gcaagaaagu cggccuucug ucucugaacu ccgauagaga gguggcuaau     3660

cgccagcaac ucauccugcc ucccgaacau agcgcccuuc ugcggcuccu gcagauuccc     3720

gacgaggauc ucgaccagau ccuggcguuc uaugauaaga acauccuugu ugagauucug     3780

caggaacuga ucacgaagau gaaaaaguuc uacccguuuu auaaagggga gagagaguuc     3840

cucauugcca auaucgaaaa cuuuaaucaa gcuaccacau cugaaaaggu gaacucccug     3900

gaggagcuua uuacccugcu ccacgccaau agcacaucug cucaccugau cuucaacaac     3960

auugagaaaa aggccuuugg acgcaaaacc cauggccuca cgcugaauaa caccgacuuc     4020

aucuaccagu ccgucacagg gcuuuacgaa acccggaucc acauugaaug a              4071


<210>  4209
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4209
augaccaaga aagagcagcc cuacaacauc ggccuggaca uugggacagg aagcgugggc       60

ugggccguca cgaaugauaa cuaugaccuc cugaauauca agaaaaagaa ccuuugggga      120

guuagacugu ucgaaggggc ucaaaccgcc aaagagacac ggcucaaucg cuccaccaga      180

cggcgcuaca gacggcgcaa gaacagaauu aauuggcuga acgaaaucuu uucugaggaa      240

cuugcgaaua cagauccuag cuuccugauu cggcuccaga acuccugggu gucuaagaaa      300

gacccggauc gcaagagaga caaauauaau cuguuuaucg auaaccccua caccgacaag      360

gaguauuacc gggaguuccc uacaaucuuu caucugcgca aagaacucau uaucaauaag      420

aacaaggcug auauuagacu gguguaucuu gcccugcaca auauccucaa auaccggggc      480

aacuucaccu acgagcacca gaaguuuaac auuagcacgc uuaauuccaa ccuuucuaaa      540

gaacugaucg agcucaauca gcaacugauu aaguaugaca ucagcuuccc cgauaacugc      600

gacuggaauc auaucuccga uauucugauc gggcgcggaa acgccaccca gaaaagcucu      660

aauauucuca acaauuuuac acuggacaag gagaccaaaa agcugcugaa ggaagucauc      720

aaccucauuc ugggcaaugu ggcucaccuu aacacaaucu ucaaaaccuc ccugacgaag      780

gacgaggaaa aacucagcuu uucuggcaag gauaucgagu ccaaacugga cgaucuggac      840

agcauucucg augacgauca guucacaguu cuggacaccg ccaauagaau cuacucuaca      900

auuacccuua acgaaauccu gaauggggag uccuauuuua gcauggcuaa ggugaaccag      960

uacgagaacc augccauuga ucucuguaag cugcgggaca uguggcacac cacaaagaau     1020

gaaaaggcgg ucggacuuuc cagacaagcc uaugaugacu acaucaacaa accuaaguau     1080

ggcacgaaag agcuguacac cucucucaag aaguuccuga aaguggcucu gcccacaaau     1140

cucgccaagg aagcugagga gaaaaucagc aaggggaccu accugguuaa accucgcaac     1200

uccgagaaug gagugguccc guaucagcuu aacaagauug agauggaaaa gaucauugac     1260

aaucagagcc aguacuaucc cuuccugaaa gagaacaagg aaaaacuccu gucuauccug     1320

uccuuucgga uuccuuacua ugugggcccc cuucaaagcu cugagaagaa uccuuucgcc     1380

uggauggaaa gaaaaagcaa cgggcacgcg cgccccugga auuuugauga gaucguggac     1440

cgggagaagu ccucuaacaa auucauuaga cgcaugacag ucaccgauag cuaccucguu     1500

ggagaaccgg ugcugccuaa aaauucccug aucuaucagc gguacgaggu gcucaacgaa     1560

cugaauaaca ucagaauuac ggagaaccuu aagacaaauc ccaccggcag ccgccugacc     1620

gucgagacaa aacagcauau cuacaacgaa cucuuuaaga auuauaaaaa gauuaccgug     1680

aaaaagcuga caaaguggcu gaucgcccaa ggcuacuaua aaaacccuau ucuuaucggg     1740

cucucucaga aggacgaguu caauuccacg cugaccacau accuggauau gaaaaagauc     1800

uuuggaagcu cuuucaugga aaacaauaaa aacuauaauc agauugagga acucaucgag     1860

uggcugacca uuuuugagga caagcagauc cuuaacgaaa agcugcacuc cagcaauuac     1920

uccuauacau cugaucaaau uaaaaagauc agcaacaugc gguacaaagg cugggggaga     1980

cucuccaaga aaauccugac cugcauuacg accgagacaa auacccccaa gucucugcag     2040

cuuagcaacu acuccguucu cgaccugaug uggacaacca auaacaacuu caucagcauu     2100

aucucuaaug auaaguauga cuuuaaaaac uacauugaaa aucauaaccu gaauaagaac     2160

gaggaucaga auaucuccaa ccucgugaau gacauccacg ucagccccgc ucugaaacgc     2220

ggaauuacgc agucuaucaa gauugugcaa gaaaucguua aauucauggg ccacgccccu     2280

aaguauauuu uuaucgaggu gacacgggag accaaaaagu ccgaaauuac aaccagcaga     2340

gggaagcgga uccagcgccu ucagucuaaa cugcucaaca aggcuaaugg auucaaaccg     2400

cagcugagaa aguaccuggu ccccaacgag aaaauccaag aagaacuuaa gaagcauaaa     2460

aaugaucuca gcuccgaacg gauuaugcug uauuuccugc agaacggcaa gagccucuac     2520

ucugaggagu cccugaauau caacaaacuu agcgacuauc agguggacca cauucugccu     2580

agaaccuaca uccccgauga cucccucgaa aauaaggccc ugguccuggc caaagagaac     2640

cagcgcaagg cugaugaccu ucugcucaac ucuaauguga uugauaagaa ccuggaacgg     2700

uggacauaca ugcucaauaa caauaugaug ggccugaaaa aguuuaaaaa ccuuacgaga     2760

cgcguuauca ccgacaagga uaaacugggg uucauccaua gacaacucgu gcagacaagc     2820

cagaugguca agggaguggc gaauauucug aacuccaugu auaagaauca aggcaccaca     2880

uguauccagg cccgggcuaa ccugucuacc gccuuucgca aagcccuuag cgaccaggau     2940

gacagauacc acuucaagca uccugagcug guuaaaaauc ggaacauuaa ugauuuucac     3000

cacgcucagg acgccuaucu cgcguccuuc cuggggacgu accgccucag acgguuuccc     3060

acagaugaga ugcugcuuau gaacggagaa uauaauaagu ucuacggcca agugaaagag     3120

cuguauucua agaagaaaaa gcucccggac agccgcaaaa acggguuuau cauuuccccu     3180

cuggucaacg gcaccaccca guacgacaga aauacaggag aaaucaucug gaacgugggc     3240

uuccgggaua agauucugaa aaucuuuaau uaccaucagu gcaacgugac ccgcaagaca     3300

gagauuaaaa cggggcaguu cuaugaccaa accaucuaca gccccaagaa uccuaaguau     3360

aaaaagcuga uugcucagaa aaaggauaug gaccccaaca ucuacggagg cuuuucuggg     3420

gauaauaaau ccagcauuac aaucguuaag aucgacaaca auaagauuaa accugucgcc     3480

auccccauua gacugaucaa cgaucucaag gacaaaaaga cccugcagaa uuggcucgaa     3540

gagaacguga aacacaagaa gucuauucag aucaucaaaa auaacgugcc gauuggacaa     3600

aucauuuauu ccaagaaagu cggccugcuu agccuuaauu cugaucggga gaucgccaac     3660

agacagcagc ugauucuccc ucccgaacau uccgcucugc uucgcauccu gcaaaucccc     3720

gacgaggauc cugaccagau ucucgccuuc uacgacaaga acauccuggu ggaaauucuc     3780

caggagcuga ucacaaaaau gaagaaguuc uauccguuuu acaaaaauga acaggaguuc     3840

cuugcuagca acauugagaa uuuuaaccaa gccaccacgu ccgaaaagau caauagccug     3900

gaggaacuga uuacccuccu gcacgcgaac ucuacaagcg cccaccuuau cuucaauaac     3960

aucgagaaaa aggcuuuugg gcggaaaacc cauggccuga cacucaauga uaccgacuuc     4020

auuuaccagu ccguuacagg acuguaugaa accagaaucc acauugagug a              4071


<210>  4210
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4210
augagcaaga aagacuccga guacuauauc ggccuggaua uugggaccaa cucuguggga       60

ugggccguca caaauggcaa guacgaccuc cugaacauca aaaagaaaaa ucuuugggga      120

guuagacugu ucgaagcugc cgagagcgcg gcugggcggc gcauuaacag auccacgcgg      180

cgcagauauc ggcgcagacg gaaucgcauc aacuggcuca augaaauuuu ugccgaugag      240

cugucuaagg uggaccccag cuuccuuacc agacugaacu ccucuuggau cagcaagaaa      300

gauuccagcc ggacacgcga caaguacaau cucuuuaugg augccgacua uaccgauaaa      360

aacuaccacc auaaguaucc uacaaucuac caccugagac gggaccugau guucgaugac      420

aaacaguuug auauucgccu cguguaccug gcccuucaua auaucauuaa guauagaggc      480

aacuucaccu acgaccaccg gaaguuuaau gucucugaaa uguccagcaa ccugacaaaa      540

gcccucgaga auuucaacca agagcugaug gaauuugagg acgcuuuccc ggauaacacc      600

gacuauucug ccaucuccga agugcuucuc aagagcaaua acguuucuac gaaaauugau      660

gagaucauuu ccaaucugga gcuggacaag gauaccaaaa aguaccugaa agaagugcuc      720

aagcugaucc uugggaacag cgcgaaucug aacacaaucu uucauaccuc ccucgacaag      780

gaugacgaga aacugucucu uagcgccaag gauauugaac ugaaacucaa uaaccuggac      840

ucccugcuca gcgaugagca gauccacuuc auuucuuccg cuaaugaacu guauagcucu      900

aucacacuua acgacauucu ggacggaaag accuaccuca gccuggccaa aaucaagcag      960

uaugauacgc acugcuccga ccuucaggag cugaaggaga ugugguacaa aacaaccaau     1020

cccgaucuca ucaagaaauc uagaaaggcu uacaaagaau auguccaugg cagcuacaac     1080

acagaggacu uuuauaaggc cauuuccccu uuccugaaga ucgcgauucc cgaagaucug     1140

gccaaagacg cucucaagaa aaucgaugcc aauaccuacc ugccuaagca acgcaccagc     1200

aacaauggcg ugauucccuu ccagcuuaac cugaucgagc ucgagaaaau cauugacaac     1260

cagucuaagu auuacccuuu ucugaaggaa aaucgggaga aaaucauuuc cauccugagc     1320

uuccgcauuc cguauuacgu ugggcccauc caggcuccua cagauaagaa cuuuucuugg     1380

auggugaaaa agauucaagc caaagccaga cccuggaauu ucgacgaagu caucgaucgg     1440

gagaagucca gcaacaauuu uaucaaaaga augacgacca cagacaccua ccuuguggga     1500

gaaccugugc uccccaagaa cucccugauu uaucagaagu ucgagguccu gaaugaacuc     1560

aacaauaucc gcauuucuaa aaaccugagc caggauccgc uuggcuccaa gcugucuguu     1620

gaggugaaac agcaaaucuu ugaggaacuc uucaagcggu acaaaacagu caagagcgac     1680

cagcugaaga aauggcugau uagaaauucc uauuuuaccg acccuacgau caucgggcuu     1740

agcaagcccu cugaguucaa cuccagccuc ucuacauuua uugauaugaa agcuaucuuc     1800

ggaauggacu uugugaauaa cccucgcaau uccagccagc uggaggaacu ggcggagugg     1860

cucaccgugu ucgaagauaa gcaaauucug ucugagaaaa uccggcacag caacuacccc     1920

uauuccgacc agcagauuag aaagaucagc aacaugcgcu accggggcug ggggagaauc     1980

ucuaagaaac uucuguguga uauuaagguu gaauccacca ucuauaaaag ccaucagcuc     2040

caccccuacu ccauucugga ccugaugugg ucuacaaaua acaauuucua cagcauccuu     2100

cacaaggaua aauauaacuu ugaguccauu cucgaagacu acaaucuggg aaccgaugac     2160

aagacagaua ucaacgagcu gguggacaau auccauacgu cuccugcccu caagcggggc     2220

auuauccaaa gcauuaacau cgucaaagag cuggugaagu ucaugggcca cgcuccgcag     2280

cauauuuuuc uugaguucac ccgcgaagau aaaacaucca cccugucuga aagcagaaag     2340

aaacggcuca agaaacugua uaauaaccug uccgaggaca caaagaaguu uaaacuugac     2400

cucagagauu accugguucc cgacaagagc auccagaaag aacuggaguc ucacgauaag     2460

gaccucucca aagagcgccu gaugcuuuau uuccugcaga auggaaagag ccucuacucu     2520

gaugaagccc uggacauuaa ccggcugucc gaguaucaag uggaucauau ccuuccuaga     2580

accuacauca aggacgauag ccuggaaaau aaagcccucg uccuggcuaa ggagaaccag     2640

cgcaaagccg acgaucuccu gcuucagccc ucuauuauca auaagaacau ggaaagaugg     2700

auuuacauga aagaccacgg gaugaucucc ccuaagaagu uuaaaaaccu gacgcggacc     2760

acaauuaccg aggacgauaa ggugcacuuc aucaaucgcc agcucgucca aacaagccag     2820

aucauuaaaa acguggcgaa uauccuggac aacauguaua aggagcaggg caccacgugc     2880

guugaaacaa aagcuaaucu guccagcgcc uuuagaaccg ccuucucuga gcaggaugac     2940

aaguaccauu uuaagcaccc cgaguucauu aaaaaccggg augugaauga cuuucaucac     3000

gcucaagaug ccuaucuugc uugucugcuc gggcuguacc gcauccagag auucccgagc     3060

gacgaaaugc uccugauuaa gaaagaguau cggaaguucu uugcccaggc gcaaaacucc     3120

gucaaaaauc acggaaagau cccugauucu cagaagaacg gcuucauccu ugcuccccug     3180

gugaaugggg aaacacucau uaacaauaaa accauggaga ccaucuggaa cauggacuac     3240

aagcgcaaag uuauuaagau cuuuaauuau aaacaguaca acauuacaaa gagaacggaa     3300

aucaaaaccg gagaguucua caagcagaca cuguauagcc cucaugauga aaaugugaag     3360

aaccccauuc cucagaaaaa gaaccuggac cccaaaaucu acggcggcua uuccgggguc     3420

caaucuagcu accuugcuau cauuaaggug gaucagaaaa agccgaccau gguguccguu     3480

ccuauccggu uuucuaagau gauugacgac ggaaaacuga gccuccaguc cuggcuggag     3540

aauaacauca agcacaaaaa gacaauugag guccucaaaa ccaagauccc caucggccaa     3600

cugacguaua aucccaagaa cggguaccuu agcgugcagu cugaaaaaga gauugccaau     3660

gcucagcagc uguuccugcc uuuugaacau guggcccucc ugacacuucu gcacgcgccg     3720

accuccgagu augaucaaau ccucagcuuc uacgacaccg auauucugcu cgaaaucuuu     3780

gacgagauua caaacaagau caaaaccuuc uuucccuucu acaucgauga gcaggaauau     3840

cuggucucua uucgcgagaa uuuccagucc gccacaguga ucgaaaaggu uaacgugcuu     3900

caacagcugc ugauuaugcu ccaugcuaau agcuccagaa ucucuauuaa acugggagag     3960

gucagcaaga aggaccuugg ccggaaaaag aaaagaaucc cucuggaaaa cacggaucuc     4020

guguaccagu ccguuaccgg gcuguaucag acaaaguuuc acauugacug a              4071


<210>  4211
<211>  4074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4211
augaacgaca gaaauaagaa auacaacauc ggccuggaua uugggaccgg aagcgugggc       60

ugggccguca cagacaauaa cuauaaucuc cugcacgcua agaaaaagaa ccuuuggggg      120

auccggcugu ucgauggagc cgagacggcg gcuucccgca gaaccuuucg gucuacacgc      180

agacgguacc gcagacggcg caauagaauu aacuggcuca augaaaucuu cgccgacgag      240

cuggccaaaa aggaucagag cuuucuuguu cggaugaacu ccucuugggu gagcaagaaa      300

gacaagauuc gccaaagaga ucaguauaau cuguucaugg acuccgauua caccgacgug      360

gaauaucauc gggaguuucc cacaaucuuc caccuccgca aaagacugau cgaagauaag      420

ucucaggcug acauucggcu ggucuaccuc gcccugcaua acaucauuaa auauagaggc      480

aauuuuaccu acgagcacca gaacuucgau gugagccaua uggacuccgg gcuugcucug      540

cuccugaauc aacuuaacca gcagcugcag gccuuugagg uugcguuccc ugacgaaaca      600

aacuuugaga gcaugucuac cauccuccug gaaaagaaga cgccgaccca gaaaauugau      660

gagaucgugg accaaauuca gcccgagaag gaucugcucc agaucaccaa aaauaucaug      720

aagaugcugc uuggaaacaa agccgaccug gucaagauuu ucucccucga uagcgacgau      780

aaaaucacag uggaauuuuc uuccaauagc auugagcaag accugucuaa ccuugaagag      840

acacuguccg augaacaguu caauaucauu acccucgcua acagcaucua cuccucuauc      900

guucugaaug acauucugag cggcgagacg uaucucuccu ucgccaaggc ucgccaguac      960

agcgaucaca agaaagaccu ggccaaacuu aaaacaaugu ggcgggaguc uccugacaga     1020

aaggcggugg gcaaagccaa gaaggcuuau gaugacuacc ugcacuccgg gaaauaugga     1080

aucgaagauu uuuacaagga caugagcaaa uuccucgaga ccgccacacc cgucgaacug     1140

gcuaaggagg ccaacgagaa aauucuuaau aagucuuauc ugcucaagca acgcaacaau     1200

gaaaacggcg ugaucccuuu ucagcugaau aaaaacgaga ugauugaaau caucaauaac     1260

caggccaagu acuaccccuu ccuggcugag aacaaagaua agcuccugag cauucuuucc     1320

uuucggaucc cuuauuacgu ggggccgcug cagucuaaaa aggacaaucc cuucgcgugg     1380

auggaaaaga aaagccaagg aaagauuaga ccuuggaacu uugaugagau cgucgaccgc     1440

gaagccucca gcaauaacuu cauucggaga augaccucua ccgauacaua ucucaucggc     1500

gagcccguuc ugccucugaa aucccccauu uaccagaagu augaggugcu uaaugaacuc     1560

agcaacgugc gcaucaaugc ugacgagguc gauaacccgc ugggggacaa acugucugug     1620

gaucucaagc aggccaucua caaugaacug uuucuuaaac aucgguccgu uagcaaggcc     1680

cugcucacga aguggcugau uaaccagucc uauuuccaaa auccucagau cucuggacug     1740

gcugacagca aaaaguuuaa cuccggccuu accacauacc acgacuucaa aaagauuuuu     1800

ggcgaggauu ucgugaauga ccccaccaac cagucucagc ucgagcaacu ggccgaaugg     1860

cugacaaucu ucgaggauaa aaagauucuc aagaucaaac uggacgauag ccaguuuaau     1920

ugcaccccug aacagaucaa gaaaauugcg gggaugagau accagggaug gggccgccuu     1980

uccaagaaac ugcucuguga caucaagacg acaaccguca ccagcaagaa ccaucaacag     2040

gcuucuuauu ccauucugga ucugaugugg aacacaaauc ggaacuucau cagcgugcuu     2100

aaaaaugaca ccuacaaguu ugaggauaaa auucagacau ucaaccucga caagaaugaa     2160

gccgccacgc cccaagcuau gaucgaugag cugcagaccu cucccgcccu gaaaagaggg     2220

auccggcagu ccguuacaau uauccaggag cucgugaagu uuaugggaca cgcuccugaa     2280

cacauuuucc uggaguuuac ccgcgaagac gaugucagcu cucuuacaaa gagcagagac     2340

ucccggcuga acaaacucua ugccagaauc gcgaagaaua ccaaaaagac ggacgaugag     2400

cugaaaaaga gccugcuucc gucuuccgac auuaaagcca cccucaaggc uaacaagaau     2460

aaccugagca augaucgccu gaugcucuac uaucugcaaa ugggcaaauc ccuuuacucu     2520

aaccagcccc uggacaucaa ucggcucagc gaauaucaga ucgaucauau ucugccucag     2580

uccuacauca aggacaacuc ucuggagaau agagcccuug ugcuggcuag cgagaaccaa     2640

cacaaauccu cugaucuccu gcucaagccc gaaauuauca accagaauau ugcccgcugg     2700

agauacauga aagacgcggg gcugauggga ccuaagaagu ucaaaaaccu uacacggauc     2760

cagauuaccg auagcgacca gaauaacuuu aucaaucgcc aacuggucca gacaucccag     2820

aucauuaagc augugagcaa cauccucgau ucuauguaug gcgaccaagg cacccagugc     2880

guugagacga gagcccaccu guccacagag uuccggaagg cuuuuagcaa ugaagacgau     2940

aacuaccauu uccagcaccc cgaauuugug aagaaucgca acauuaauga cuaucaccau     3000

gcccaggaug cuuaccuggc cugucuucug gggcucuauc aaaugaaaaa guacccgacc     3060

gacaacauga uccugcucaa gaaagaguau aagagauucu ucucuaauac caaaaagauu     3120

uacgauaaaa agcaccauuu uccugacuac gucaagaacg gauggaucau cggcuccaug     3180

uucaaugggg agaaauaugu ggaugaagag acaggacaga uuaucuggga ccagcagacc     3240

aaggauaugg uuagcugcau uuuucaauac aaacaguaua acgugacaaa gcggacggaa     3300

gccgcuaaug gcgaguucua caaccagacc gucuauaaaa aggaccccaa cgagaagcgc     3360

aaacugaucu cucuuaagca ggggcuggau acaaaacucu acggcggaua uuccggcgac     3420

aauccugcgu acauugugcu ggugaagauc gaugacaaaa agaacaaacu gguuaagaug     3480

cccaucagac uugccgaaca agucgacaau aagaccauug aucugcagac auggcucgag     3540

gaaaacgugc cucacaaaaa ggggauccag gugcugaaaa ccaagauucc ccucggacag     3600

gucaucuaca gcaaaaagaa uggcuaucug gcucuuaagu ccgacacgga gauugugaac     3660

gcccaacagc ugguucugcc guacggguau guggcccucc ugacacuucu gcagaaaaug     3720

ccugaaaaua aguacgauga gguccucgcu uguuaugacg auggaauccu ggacaacauu     3780

uuuguggcca ucaucgauaa aaugaagcac uucuaccccu auuacagcgg cgagcucaaa     3840

acccugucug acaauauuga uaacuuuaag gaaagccccg acguuaauaa gaucaaaacc     3900

cuucaacagc ugcugcuccu gcuucaugcg uccucuacaa ccgcuagcau ugaguucaac     3960

aaugugaaga aaaacucccu ggggcggaag ccuaauucua ucaacauuca gaacacagau     4020

cuccugagcu uuuccgccac gggccucuac gaaucucgca uccacaucga cuga           4074


<210>  4212
<211>  4074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4212
auggccgcua agaacuacaa uaucggccug gacauuggga ccagcuccau cggaugggcc       60

auuguggaug acaacuauca gcucaugcac gcgaaaggca aguacgggua uggaacaaga      120

aucuacucug agggccaaac ggcugccgau cggcgcgggu ucagaaccac acggcgcaga      180

cugaaucggc gcaaauggag acuucggcug cuccaggacu uuuucgaacc cuauauucug      240

ccucaggaug aggccuuuuu cauccgccag aagcaaagca accuuguccc gcaggacacc      300

aaaaagcagu accccgaauc cguucuguuu gaugacacau cugauaagga cuucuauacc      360

gaguacccua caaucuacca ucucagacag gcucugauga ccgaaaaucg gcaauuugau      420

auucgcgagg uguaucuggc cauccaccau auugugaaau acagaggaca cuuccucaac      480

aaugcucccg ucaagacguu uaaaagcggc aaccuggacc uugccaucaa guucaaugag      540

cugaacgaag aguuuaaauc cguguauccu gaucucgaac ugcaguucaa ucuugaccag      600

auugacgaga ucaagaagau ucugcucgau ucuaccaaaa gccgguuuga ccgccagggc      660

gaggcgguua agcugaucua cacagugucc gaagucaaaa ccaucgagaa gcugcaaaac      720

aaaauuguga agcaguuccu cagcgggauc cugggacuua auacaagauu cgauguucug      780

accaacacgg aaucugacga uuccaugugg aaauuuagcc ucgacucuga guccagcgau      840

gacgcccugg augaaauuau cgcucagcuu gacgaucccg ccauucaugu ggucgagcug      900

gugaaugaga uccacucuuc caucacacuc gcuggcauug ugccugaagg gcaaggacug      960

agcgagucca uggucgaaaa guaugaggcc cacaaggcgc aucugcagcu ccugaaagcc     1020

guugaaaaac uuguggacaa gaaggcugcc gacaaacugg agguggcuua cgaucaguau     1080

aucaacggca aggacucuaa gcaguucauu aaagaggauu uuuacaaggc cguccaaagc     1140

gaaaucaaag ggguggccgg agacgagguu gcugaaauua accagcucau cgagcuggau     1200

caguucaugc cgaagcagcg gaccaaagaa aauggcgcga ucccccacca acuucaucag     1260

cacgagcugg acgugauuau cgagaaccag uccaaguacu augauuggcu cggcgaaaag     1320

gucgggaaug aguacaaacu ggacaagcug gugagcuuua gaguuccuua uuacguggga     1380

ccccucgucg auacagccuc uaaagaaaag aaagacgaga cccgcuucgc uuggauggug     1440

cggaagaacc cugaguccac cgaagccauu acacccugga auuuugagga uaucgucgac     1500

aagaacgcca gcgcugaaaa uuucauuaaa agaaugacga ccaaggauaa auaucugcuu     1560

aacgaggacg ugcugccgaa gugcucucuc cugacacagg acuuugaagu uuacaaugag     1620

cugaacaaua uccgcgugaa cggcgagaaa uucaccgccg aacaaaagca gcuuauucac     1680

gaugagcucu uuacaaaaca ugcgagcguc uccaucaagc agcugaagga cuuccuggug     1740

ucugaacuca uguauguucg ggauaaucug aaaguggagg ggcuugcuga cgaaaagaaa     1800

cugaugagcu cccucagcac ccaguuugau uaccaaaaga ucauuccuga ccagaucaac     1860

gaugucaaau acagagagga cauugagcag aucauugaau ggucuacgau cuucgaggau     1920

gccgccaucu ucaagacaaa gcugaaagaa auugacuggc ugaccgauga gcaacgcaau     1980

aaacuugcua acaagcggua uagaggaugg ggcucccuca gcaagcgccu gcuggccugu     2040

cucguggacg aaaacgggca gcggaucauu gauauccuga gagacucuuc cgacaauuuu     2100

augaugauug ugaaccgccc cgaguucaaa caggcuaucu uugaugagaa ugccaaccuu     2160

accgaaacaa auagcaagaa ggacauguuc gagaugaucg auaacuuuua caccuccccu     2220

cagaauaaaa aggcgauucg gcaaguucug cucgucgugc aggacaucca gaacgccaug     2280

ggaggcgugg cuccccagaa aauuuucauc gaauuugcca gagaggauga ggucaauggc     2340

cggcgcacaa acucuagaua uagccaaauu cgggaaaugu acuccaaggu gacggacgau     2400

guuaucaaua aaaccgugag aggggagcug gaagcuaaca agggaucucu gaauaaggag     2460

aaacuuuucc ucuauuuucu gcagggcggg aucgaccugu acagcggaaa gacaauuaac     2520

aucgauaaua uuuccagcua ugacgucgau cacgugcucc cccagucuuu caucaaagac     2580

gacuccauua acaacaaggu ucuggugaau gccaaaggca accgcgucaa gaaugauaac     2640

gugccuaugg aacuuuuugg ggcgaaaaug uacccgaccu ggaagcggau gguugacaca     2700

ggcaucauga gcaaggccaa auucaagaau cugacccuca accccgauaa uguggacaaa     2760

uuuaaggcug agggauucau uaacagacag cuggucgaga cgcgccaaau caucaaacug     2820

guggcccaga uucuuaauga uaagcucggc gacgauacca agaucguguc ugucaaagcu     2880

aaccugacac augacaugag aguuuucuuu gauuucccua agaaucggaa cgugaauaac     2940

uaccaccaug ccuuugacgc cuaucugucc gcuuucgugg ggaccuaccu cuauaaacgc     3000

uaccccaagc ugcagccuua uuuugucuac ggagaguuca aaaagaugga uaaacuuaag     3060

aauaugacca gcuuugacuu ccugagagau cucaagcaca cagacaaagu gguugacaag     3120

gauacgggcg agauuaucgc ggacaaacug gccgugauua caaacaugaa caagaucuau     3180

gauuuuaaga aaguccuggu gacccacgaa guucaugaaa auucuggggc uauguacgac     3240

caaacacuuu accccgccaa gcuggauaaa agccucggac ggaagggcgc caaacagcug     3300

aucccgaaga aggacgauau gccuaccgcu cucuaugggg gcuacaccgg auccaaagac     3360

gccuucauga gcauugugcg ccugaagaaa aaggaugaag ucuauuacaa agugaucggc     3420

auucccguua gaaucgcgga cucugugcag cacgggaagg aggucgaugu ggaggcucuu     3480

acacaguuua uugaaacgaa auucaccaag gagaaccuga auaagaaaac aggagaaauc     3540

acccggaagg uggagugguu cgaacucguc cugccuaaag uucuguuugg ccaagccgug     3600

uucgacgggg gacagcccuu uaugcuuggc uccagcacau auccucauaa ccugcgcgag     3660

cucgugcuga ccaaggauuc cgucaaaaag aucgccggga aggagucuaa uaaaagcuac     3720

cucgacgcuu augacgaaau uauggaggcc augaacaagu acuucccccu guacgauacg     3780

aauaaauuua gagaaaaggu gaucgagggc cgguccaaau ucauugaccu uccggauaag     3840

gacgaguaug aaaacaauaa gcugguucag gugggaaaau cuacaaucau ugaucgcaug     3900

uuuaucggcc uccacgcgaa cgcugccaga accgaccuga agaauauugg gaucaccaca     3960

ccuuucggau uuaugcagca agccagcggc aucaaacuga ccaaggauac agucgugcuu     4020

uuccaguccu cuacggggcu guuugagcgg aaaguugcuc ucaaggaccu guga           4074


<210>  4213
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4213
augaaggagc ccuacggcgu ggggcuggac aucggaacca acagcgucgg cuggacaguu       60

guggaugccu ccgggcacgu gagaaaaauu aagggacaga cgggcaucgg ggucagacuc      120

uucaaagaag gagcugccgc ggcugaccgc agaggcuuuc ggaccacacg cagacggcug      180

aagcgcguga aauggagacu ucggcugcuc cgcgaguucu uugaucaacc uauuucuaag      240

guugacauca auuucuuugc cagacggaag uauagcgaug uguccccgcg cgaccccaac      300

uacaauggcc uggaaaagac ccuuuucaac gauagaucug accaggauuu uuaucaugac      360

uacccuacaa uuuaucaucu gcgggaggcc cucaugaccc agcaccgcaa guucgauguc      420

agagaaaucu accuggcuau ccaccauauu gugaaauauc gggggaauuu ucugagaaac      480

gacgccgcua cagccuaccg cagcggaacc cucgaucugc agcaacacuu cgagacgcuu      540

aaucaccugu uuucccaggc ggaccucgag cugaaccuua aucugaccac agacguugcc      600

cuccuggauu cuaucaagca gacccuggug cggacagaca uuagcagauc cgaucgccag      660

aaacucauca ugccccugcu ugcuguccug accggcgcca cgacagcuga aaagaagcgg      720

caaaaagccg uggugaccga guucgcgaag gcccucgucg ggaacaaaac aaagauugac      780

guucugaccc uuaccgauau cgacgcuaca gaagccaaag auugggcuuu uagccuggag      840

gagaaucagg acaagcuccc uggaauugaa gauagauucu cugagguggg ccagcagauc      900

aucgacgaag ugauucgccu guacgccucc gucaaccugg cccaacucau ccccgagggg      960

aaacgguuca gccagucuau gguggaaaag uauaagagac auggagagga ucugaaacuu     1020

cugaaggcuu acauucgcuc ccagagcgac gcgaaacggg gcagagccau ccgcgcuacg     1080

uaugaccaau acauugaugg cguuaagucu aaacagguga cccaggaggc cuuucagaag     1140

gcccucggga aguucgucga cgcugaugug gaccuuaauu ccugggccgc gcaaaucaaa     1200

caggcuaucg auagcgaaca guuuaugccu aaacugcgga caaaacagaa cggagccauu     1260

ccguaucaag uucagcagaa ugagcuggac cagaucauuu ccaaccaaaa ggaauacuau     1320

cccuggcucg gcgaggaaaa cccugugauc aaaagacggg ggaaguuccc cuacaagcug     1380

gaugagcugu uuggauuccg cgucccuuac uaugugggcc cccucauuac caaagagaug     1440

caggccgcua cagccaaugc uggguuugcc uggaugguca gaaaggcgga cggaccgauc     1500

accccuugga acuucgauga caaaguggau cggguugccu cugcuacggc cuuuauccag     1560

agaaugcaga caaccgacac cuaccugauu ggcgaagagg ugcuucccgc ucgcagccug     1620

aucuaucaac gguucauggu ccucaaugaa cugaacaaua uuagagugga ugacggccag     1680

cugacaccuc gccagaagca aagacuuuac aaccagguuu uuaaaaagca caagcgggug     1740

accgccaaaa auauccagga caaccucguc gcggcccgcg aguuccccac agcucccaga     1800

auuacggggc uggccgaucc uaagguguuu aauuccagcc ugucuaccua ucaugaccuc     1860

aaacaggugc ugccggaagc uauguccgau guucggaagc aagccgacau cgagaaaauu     1920

auccuuugga gcacaaucuu cgaggauucu accauuuuca agcagaaacu gacagaaauc     1980

agcuggcuca ccgccgcuca gcugaagcag cugggacaac uucgcuacag aggcuggggg     2040

cagcuccugc ggaagcugcu cacgggacug aaagacgaua acaaucgcuc cauuauggac     2100

ggccuuuggg agaccaacgc gaauuuuaug cagcugagau cucagcccgc caucgaaaac     2160

caaauuaaga gcgcuaauca ggccaaccuc acaaccgccg aucugcagga cacaaucaac     2220

gagcuguaua ccuccccuca aaauaaaaag gcuauccggg aggucauggu ggugcuugcc     2280

gauauuaaag acgcgaugca cgggcagacg cccaacugga ucuucgucga agcugcccgc     2340

ggcggaggcg uggccgggag acggacacag agcagaucua agcagauugu ugcugccuac     2400

caaggaaccg cgcaggcuau cgugcgcgau cagguccagc gggagcucaa ggagaaaauu     2460

aagaauaaag ccaacuuuaa uacaagacug gugcuguacu uccuccaaaa cggccgcgac     2520

cuguauaccg gggacgccau caauaucgau cggcuuuccg aauacgacau ugaucacauc     2580

cugccucaga gccucguuaa ggacgauucu cuggacaaca gagugcugac caaugcucgc     2640

auuaaccggg agaaaaauga uacauuugcc uccgaaaagu ucggaaagaa aaugggcgcu     2700

aaguggaaag agaugcauag aauggggcuu augacgaagc gcaaacucga ccaucugcug     2760

augcggcccg augaaaucag caaguaugcc accggcuuua uuaacagaca gcucgucgag     2820

acacgccagg ugaucaagcu gguugaggaa cuuaucaaug cggaauaccc guccaccgcc     2880

auugugucug ucaaagcuaa ccugacacac caauuccggc agaccuuuga cuucccuaag     2940

aucagagagg ugaaugauua ucaccaugcc uuugacgcug cccucacggc cuucauugga     3000

acauaccugc ugaaacggua ucccaaacuu gagcgcuuuu ucguguacgg caaauucgcu     3060

aagcagccug ucaaccugac cagauuuaac guuauccgga aacucgcgaa gaccgacaca     3120

cccauuaccg auaucgacac aggggaagug cugugggaua agcucacgga cauuaaauac     3180

uucgagaccc uguaugauua caagagacuu cuggugacac gcgaagucag cgagaauuau     3240

ggagccaugu uugaccagac ccuccugaag gcuuccgaua acggcaagcg gcugauccgg     3300

aaaaagcaau cuuucgacac agccaucuac gggggauaua ccggcaagac gcuugccuac     3360

auggcuauug ugaaaguuag caccaagaaa gaugugcagu aucaggucgu ggggguuccu     3420

acaaccaugg ugacacaaau cgaacagcug gagcagcagg gcaugaccaa uaagcaagcc     3480

gucacaaaag ugauugagcc ccucaucgcg gcuaacaaua agaagaugcu gaccuacgaa     3540

guucuccugc cgaaagugcg cuuugagcag gucgugcggg acagaauuaa gggacagacg     3600

caccgcuucg cccuuggcac agauaccuac uaucacaaca uccaggaacu guaccucccu     3660

cugacccugc aacgggccuu ucuugggaaa cagaaugagu ccgaggugca gcugaaccag     3720

aaucuccuga cagucuucga cgcuacccuc gcccaaguua acagauauuu uccccuguac     3780

gaaaugaaua aguucaaaaa gauccuuaac agcgcgcgcg aucagauuga gcugcuggaa     3840

gcuaagaauc aguuugugaa aaacaagcuc gugcugggaa aacaaggcau ccuuacgaau     3900

auguucauug ggcugcaugc caacgccucu uauggagacc ucaagguccu gggcgugaaa     3960

aucgacuucg ggaagcucca gguucccggc ggaauuaagc ugacaggcga ugcugagauc     4020

aucuaccagu ccccuaccgg gcuuuuugaa agaaaaauug cccugaagga ccuguga        4077


<210>  4214
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4214
augaacgagc ccuacggcgu ggggcuggac aucggaacca auagcgucgg cuggacaguu       60

guggauauga acgggagagu gcggaagguc aaaggaaaga cggcccucgg cgcucgccug      120

uucaaagaag gggccaccgc ggaggacaga cggggauuuc gcacaaccag acggcgccuu      180

aagagaguga aauggcggcu gcgccuccug agagaguucu uugaucagcc uauuuccaag      240

aucgacccga auuucuuugc ucggcgcaag uauucugaua uuagccccag agacccuaac      300

uacaauggcc uugccaaaac acuguucaac gaucggaccg accaagaauu uuaucacgau      360

uaccccacaa ucuaucaucu ccgcgacaag cugaugaccu ccgauagaaa auucgacauu      420

cgggaaaucu accuggccau ccaccauauu guuaaguaua gaggcaauuu ucuccgcacg      480

gggccugcuu cucaguacgg aagcgccucc cugcaccuug cuaccucuuu ccagaaacug      540

aacgaucucu uugcccagag cgaggagucc cugaauuuca agcuggugac agacgaagcg      600

caacuucagc agauccagca aauucucguc cggaccgacc ugagcagauc ugagcagcag      660

cgccagaucu ggccccugau ggugguucuc augggcgcca cagcugccga aaagaaacgg      720

caaaaggauu uugcuaaaga gcuggccaac gcgcuugugg ggcugaaggc caaacucaau      780

gucgugaccc ugacgaacgu ggacacaacc gucguuaagg auuggacauu cacccuugag      840

gaaucccagg acaaacugcc ugagauugag gaacagcuca gcgaaguggg acaacagauc      900

auggaugagg ugauucagcu guacgcuucu gucaaucugg cccagcucau cccggcuggc      960

aagagauucu cccaacauau gguggagaag uauaaccacc acgaaaagaa ucuggagcuu     1020

cugaaggccu acauccauag ccagucugac uccaaacgcg ggcgggaaau uagagccacc     1080

uaugaucgcu acaucgacgg aguuaagagc aaacccguga cacaggagau guuuuauaag     1140

gaucucauga aguacgucga agcugacgcg acguccaacc accuggccgc ugagauuaaa     1200

gaugagaucg acucugaaca guucaugccu aaacuucgga ccaaacaaaa cggcagcauu     1260

cccuaucagg ugcagcagua cgagcuggac caaaucaucu cccaucagaa gaaauacuau     1320

ccuuggcucg gcgaagagaa ucccguugcc gaaagacgcg ggaaguuucc guacaagcug     1380

gaugagcugg ugggauuccg ggucccuuau uacgugggcc cccucauuac aaaagaggac     1440

cagcaggcca ccagcggggc uggauuugcc uggaugguca gaaaggcgga uggcccuauc     1500

acacccugga acuucgacca aaaaguggau cggauugcuu cugccaccgc cuuuauccag     1560

cgcaugcaga cgacagacac cuaucugauu ggggaagaug uucuucccgc uagaucccug     1620

aucuaccaac gguucauggu gcucaaugag cugaacaaua ugagagucga agaccagaag     1680

cuggccccuc gccagaaaca gcggcuuuau aaccaagugu uuaagcagca ccagcacguu     1740

agcgugaaga auauccagca aaaccucaug gaugcuggag aguacagaga gaccccgaaa     1800

auuacaggcc uggccgaccc caagggcuuc aauucuagcc uguccaccua ccaugauuuu     1860

aaaaagaucc ucccugaagc gauugccgac gagcacaaac gcgcugauau cgaaaagauu     1920

auccuguggu cuacaacguu cgaggacagc gccauuuuua aacagaaacu ugaagagguc     1980

gcuuggcuga ccgacgccca gagaaagcag cucuccgggc ugcgguaucg cggauggggc     2040

caacugagcc auaaacuucu cacagcguuc aaggaugaca acgggagauc ugugauggau     2100

ggacuguggg agaccuccga caauuucaug cagcugcgga aacagcccgc caucgaaaca     2160

caaaucaagg aggcuaacca ggccaaucuc accgaugcug acauucagga uacgaucaac     2220

gaacuguaca ccagcccuca gaauaaaaag gccauucgcg aggugaugcu uguucuggac     2280

gauaucaaga acgccaugca cggccaaaca cccucuugga uuuuuaucga agcugcgaga     2340

gggggcggag ucgccggccg gcgcacccag uccagaagca aacagaucgu ggaggcuuau     2400

aaguccacag ccaaagagau ugugucugaa aagguccagc augagcucaa cgaaaagauc     2460

aaggccaagg cugacuucaa uacccggcug gugcuguacu uucuucaaaa cgggcgcgau     2520

cucuauacga augaggccau uaacaucgac agacugagcg aauacgauau ugaccacauc     2580

cugccgcagu cccucguuaa agacgauucu cuggacaauc gggugcuuac aagcgcgaga     2640

aucaaccgcg agaagaauga uaccuucgcu uccgagaaau uuggacggaa gaugggcgcc     2700

caguggagag aacugcaccg caacgggcuc augacacagc ggaaacugaa gcaucugcuu     2760

augagaccug acgagauuag caagcacgcc accgauuuca ucaaucgcca acucgucgaa     2820

accagacagg ugauuaaacu gguugaggag cugaucucuu ccgaauaucc cagcgcuucu     2880

auuguggccg ucaaggcgaa ccucacacau caguuucggc agacguucaa uuuuccuaaa     2940

cugcgcgagg ugaacgacua ccaccacgcu uucgaugccu cccuuaccgc cuuuaucgga     3000

auguaccugc ucaagcaaua ucccaaacug gaacgguucu uuguuuacgg caaguucgcu     3060

aaacagccua ucaaucugac aagauucaac gugauucgga aacuugccgu cgcugaggaa     3120

cccaucgcca auauugagac cggggagauc cugugggaca agacagcgga uauuaagugc     3180

uuugaaaagc ucuauaacua caaacgccug cucgugaccc augaggugag agaaaacuau     3240

ggcgccaugu ucgaccagac gaucuuuaag gcuacagaua gccggaaacg ccugauucaa     3300

aagaagaaag gauucgccac cgagaccuac ggcggguaua caggaagaac ccuugcuuac     3360

cuggccaucg ucaagguugu gacaaauaaa gacacgcagu accagguggu cggcaucccg     3420

accacaaugg ugacccagau ucaacagcuc gaagccucug ggcugacaag caagcaggcu     3480

guuaccaaag cgaucggagc caugauuaac gagaauaaca agaagcugca guccuaugag     3540

gugcuucugc cuaaaguccg guuugaacaa gugguuagag acgaggugaa gggccacauc     3600

caucgcuucg cucucgggac ggauaccuac uaucacaaua uucaggaacu guaccucccc     3660

cugacaaucc agcgggccuu ucuuggcaaa agaaacgaca ccgaugccca gacagagcaa     3720

aaucugcuca ccgucuucga aacaacccug gcucagguga accgcuauuu uccccuguac     3780

gagaugaaua aguucaaaca gauccuuaac agcgcccaag agaaguuugc ggcucugccu     3840

acgaagaaua aaacaguuaa gaacaaacuc gugaccggaa aggacggcgu ccugaauaac     3900

cucuucauug ggcugcaugc caaugccucu cuuggagauc ugaaaauccu gggcaugaag     3960

accggguucg gaaagcucca ggugccgggc gggcugaaac uuacagacca ggcugaaauu     4020

aucuaucagu cccccaccgg ccuguuugag cggaaggugg cccucaaaga ucuguga        4077


<210>  4215
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4215
augaacgagc ccuacggcgu ggggcuggac aucggaacca auagcgucgg cuggacaguu       60

guggauauga acgggagagu gcggaagguc aaaggaaaga cggcccucgg cgcucgccug      120

uucaaagaag gggccaccgc ggaggacaga cggggauuuc gcacaaccag acggcgccuu      180

aagagaguga aauggcggcu gcgccuccug agagaguucu uugaucagcc uauuuccaag      240

aucgacccga auuucuuugc ucggcgcaag uauucugaua uuagccccag agacccuaac      300

uacaauggcc uugccaaaac acuguucaac gaucggaccg accaagaauu uuaucacgau      360

uaccccacaa ucuaucaucu ccgcgacaag cugaugaccu ccgauagaaa auucgacauu      420

cgggaaaucu accuggccau ccaccauauu guuaaguaua gaggcaauuu ucuccgcacg      480

gggccugcuu cucaguacgg aagcgccucc cugcaccuug cuaccucuuu ccagaaacug      540

aacgaucucu uugcccagag cgaggagucc cugaauuuca agcuggugac agacgaagcg      600

caacuucagc agauccagca aauucucguc cggaccgacc ugagcagauc ugagcagcag      660

cgccagaucu ggccccugau ggugguucuc augggcgcca cagcugccga aaagaaacgg      720

caaaaggauu uugcuaaaga gcuggccaac gcgcuugugg ggcugaaggc caaacucaau      780

gucgugaccc ugacgaacgu ggacacaacc gucguuaagg auuggacauu cacccuugag      840

gaaucccagg acaaacugcc ugagauugag gaacagcuca gcgaaguggg acaacagauc      900

auggaugagg ugauucagcu guacgcuucu gucaaucugg cccagcucau cccggcuggc      960

aagagauucu cccaacauau gguggagaag uauaaccacc acgaaaagaa ucuggagcuu     1020

cugaaggccu acauccauag ccagucugac uccaaacgcg ggcgggaaau uagagccacc     1080

uaugaucgcu acaucgacgg aguuaagagc aaacccguga cacaggagau guuuuauaag     1140

gaucucauga aguacgucga agcugacgcg acguccaacc accuggccgc ugagauuaaa     1200

gaugagaucg acucugaaca guucaugccu aaacuucgga ccaaacaaaa cggcagcauu     1260

cccuaucagg ugcagcagua cgagcuggac caaaucaucu cccaucagaa gaaauacuau     1320

ccuuggcucg gcgaagagaa ucccguugcc gaaagacgcg ggaaguuucc guacaagcug     1380

gaugagcugg ugggauuccg ggucccuuau uacgugggcc cccucauuac aaaagaggac     1440

cagcaggcca ccagcggggc uggauuugcc uggaugguca gaaaggcgga uggcccuauc     1500

acacccugga acuucgacca aaaaguggau cggauugcuu cugccaccgc cuuuauccag     1560

cgcaugcaga cgacagacac cuaucugauu ggggaagaug uucuucccgc uagaucccug     1620

aucuaccaac gguucauggu gcucaaugag cugaacaaua ugagagucga agaccagaag     1680

cuggccccuc gccagaaaca gcggcuuuau aaccaagugu uuaagcagca ccagcacguu     1740

agcgugaaga auauccagca aaaccucaug gaugcuggag aguacagaga gaccccgaaa     1800

auuacaggcc uggccgaccc caagggcuuc aauucuagcc uguccaccua ccaugauuuu     1860

aaaaagaucc ucccugaagc gauugccgac gagcacaaac gcgcugauau cgaaaagauu     1920

auccuguggu cuacaacguu cgaggacagc gccauuuuua aacagaaacu ugaagagguc     1980

gcuuggcuga ccgacgccca gagaaagcag cucuccgggc ugcgguaucg cggauggggc     2040

caacugagcc auaaacuucu cacagcguuc aaggaugaca acgggagauc ugugauggau     2100

ggacuguggg agaccuccga caauuucaug cagcugcgga aacagcccgc caucgaaaca     2160

caaaucaagg aggcuaacca ggccaaucuc accgaugcug acauucagga uacgaucaac     2220

gaacuguaca ccagcccuca gaauaaaaag gccauucgcg aggugaugcu uguucuggac     2280

gauaucaaga acgccaugca cggccaaaca cccucuugga uuuuuaucga agcugcgaga     2340

gggggcggag ucgccggccg gcgcacccag uccagaagca aacagaucgu ggaggcuuau     2400

aaguccacag ccaaagagau ugugucugaa aagguccagc augagcucaa cgaaaagauc     2460

aaggccaagg cugacuucaa uacccggcug gugcuguacu uucuucaaaa cgggcgcgau     2520

cucuauacga augaggccau uaacaucgac agacugagcg aauacgauau ugaccacauc     2580

cugccgcagu cccucguuaa agacgauucu cuggacaauc gggugcuuac aagcgcgaga     2640

aucaaccgcg agaagaauga uaccuucgcu uccgagaaau uuggacggaa gaugggcgcc     2700

caguggagag aacugcaccg caacgggcuc augacacagc ggaaacugaa gcaucugcuu     2760

augagaccug acgagauuag caagcacgcc accgauuuca ucaaucgcca acucgucgaa     2820

accagacagg ugauuaaacu gguugaggag cugaucucuu ccgaauaucc cagcgcuucu     2880

auuguggccg ucaaggcgaa ccucacacau caguuucggc agacguucaa uuuuccuaaa     2940

cugcgcgagg ugaacgacua ccaccacgcu uucgaugccu cccuuaccgc cuuuaucgga     3000

auguaccugc ucaagcaaua ucccaaacug gaacgguucu uuguuuacgg caaguucgcu     3060

aaacagccua ucaaucugac aagauucaac gugauucgga aacuugccgu cgcugaggaa     3120

cccaucgcca auauugagac cggggagauc cugugggaca agacagcgga uauuaagugc     3180

uuugaaaagc ucuauaacua caaacgccug cucgugaccc augaggugag agaaaacuau     3240

ggcgccaugu ucgaccagac gaucuuuaag gcuacagaua gccggaaacg ccugauucaa     3300

aagaagaaag gauucgccac cgagaccuac ggcggguaua caggaagaac ccuugcuuac     3360

cuggccaucg ucaagguugu gacaaauaaa gacacgcagu accagguggu cggcaucccg     3420

accacaaugg ugacccagau ucaacagcuc gaagccucug ggcugacaag caagcaggcu     3480

guuaccaaag cgaucggagc caugauuaac gagaauaaca agaagcugca guccuaugag     3540

gugcuucugc cuaaaguccg guuugaacaa gugguuagag acgaggugaa gggccacauc     3600

caucgcuucg cucucgggac ggauaccuac uaucacaaua uucaggaacu guaccucccc     3660

cugacaaucc agcgggccuu ucuuggcaaa agaaacgaca ccgaugccca gacagagcaa     3720

aaucugcuca ccgucuucga aacaacccug gcucagguga accgcuauuu uccccuguac     3780

gagaugaaua aguucaaaca gauccuuaac agcgcccaag agaaguuugc ggcucugccu     3840

acgaagaaua aaacaguuaa gaacaaacuc gugaccggaa aggacggcgu ccugaauaac     3900

cucuucauug ggcugcaugc caaugccucu cuuggagauc ugaaaauccu gggcaugaag     3960

auuggguucg gaaagcucca ggugccgggc gggcugaaac uuaccgacca ggcugaaauc     4020

auuuaucagu cccccacagg ccuguuugag cggaaggugg cccucaaaga ucuguga        4077


<210>  4216
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4216
augaaggagc ccuacggcgu ggggcuggac aucggaacca acagcgucgg cuggacaguu       60

guggaugccu ccgggcacgu gagaaaaauu aagggacaga cgggcaucgg gguccgccuc      120

uucaaagaag gagcugccgc ggcugaccgg agaggcuuuc ggaccacacg cagacggcug      180

aagcgcguga aauggagacu ucggcugcuc cgcgaguucu uugaucaacc uauuucuaag      240

guugacauca auuucuuugc cagacggaag uauagcgaua uuuccccgcg cgaccccaac      300

uacaauggcc uggaaaagac ccuuuucaac gauagaucug accaggauuu uuaucaugac      360

uacccuacaa ucuaucaucu gcgggaggcc cucaugaccc agcaccgcaa guucgaugug      420

agagaaaucu accuggcuau ucaccauauc gucaaauauc gggggaauuu ucugagaaac      480

gacgccgcua cagccuaccg cagcggaacc cucgaucugc agcaacacuu cgagacgcuu      540

aaucaccugu uuucccaggc ggaccucgag cugaaccuua aucugaccac agacguggcc      600

cuccuggauu cuauuaagca gacccugguu cggacagaca ucagcagauc cgaucgccag      660

aaacucauua ugccccugcu ugcugugcug accggcgcca cgacagcuga aaagaagcgg      720

caaaaagccg ucgugaccga guucgcgaag gcccucgugg ggaacaaaac aaagaucgac      780

guccugaccc uuaccgauau ugacgcuaca gaagccaaag auugggcuuu uagccuggag      840

gagaaucagg acaagcuccc uggaaucgaa gauagauucu cugagguugg ccagcagauc      900

auugacgaag ugauccgccu guacgccucc gugaaccugg cccaacucau ucccgagggg      960

aaacgguuca gccagucuau ggucgaaaag uauaagagac auggagagga ucugaaacuu     1020

cugaaggcuu acauccgcuc ccagagcgac gcgaaacggg gcagagccau ucgcgcuacg     1080

uaugaccaau acaucgaugg cgugaagucu aaacagguua cccaggaggc cuuucagaag     1140

gcccucggga aguucgugga cgcugauguc gaccuuaauu ccugggccgc gcaaaucaaa     1200

caggcuauug auagcgaaca guuuaugccu aaacugcgga caaaacagaa cggagccauc     1260

ccguaucaag ugcagcagaa ugagcuggac cagauuauca acaaccaaaa ggaauacuau     1320

cccuggcucg gcgaggaaaa uccuguuauu aaaagacggg ggaaguuccc cuacaagcug     1380

gaugagcugg ugggauuucg cgucccuuac uaugugggcc cccucaucac caaagagaug     1440

caggccgcua cagccaacgc uggguucgcc uggaugguca gaaaggcgga cggaccgauc     1500

accccuugga auuuugauga caaaguggau cggguugccu ccgcuacggc cuucauucag     1560

agaaugcaga caaccgacac cuaccugauc ggcgaagagg ugcuucccgc ucgcucucug     1620

auuuaucaac gguuuauggu ccucaacgaa cugaauaaca ucagagugga ugacggccag     1680

cugacaccuc gccagaagca aagacuuuac aaucagguuu ucaaaaagca caagcgggug     1740

accgccaaaa acauucagga caaucucguc gcggccgggg aguuucccac agcuccccgc     1800

aucacgggac uggccgaucc uaagguguuc aacagcuccc ugagcaccua ucaugaccuc     1860

aaacaggugc ugccggaagc uaugucugau guuagaaagc aagccgacau ugagaaaauc     1920

auccuuuggu ccacaauuuu ugaggauagc accaucuuca agcagaaacu gacagaaauu     1980

ucuuggcuca ccgccgcuca gcugaagcag cugggccaac uucgguaccg cgggugggga     2040

cagcucagca gaaagcugcu gacgggccuc aaagacgaua auaaccgguc caucauggac     2100

gggcuguggg agaccaaugc gaacuucaug cagcuucgcu cucagcccgc cauugaaaau     2160

caaaucaaga gcgcuaacca ggccaaccug acaaccgccg aucuccagga cacaaucaau     2220

gagcuguaua ccuccccuca aaacaaaaag gcuauuagag aggucauggu ggugcuggcc     2280

gauaucaaag acgcgaugca cggccagacg cccaauugga uuuuugucga agcugcccgg     2340

ggaggcgggg uggccggacg cagaacacag agccggucua agcagaucgu ugcugccuac     2400

caaggcaccg cucaggugau ugucuccgau caggugcaga gagagcuuaa ggagaaaauc     2460

aagaacaaag cgaauuucaa cacacgccuc guucuguacu uucugcaaaa ugggcgggac     2520

cucuauaccg gagacgccau caacauugau agacugagcg aguacgacau cgaucacauu     2580

cuuccucagu cucuggugaa ggacgauucc cucgacaauc gcguccugac caacgcccgg     2640

aucaauagag agaaaaacga uacauucgcu agcgaaaagu uuggcaagaa aaugggggcc     2700

aaguggaaag agaugcaucg caugggccug augacgaagc ggaaacuuga ccaccuccug     2760

augagacccg augaaauuuc caaguaugcu accggauuca ucaaucgcca gcugguggag     2820

acacggcagg uuaucaagcu cguggaggaa cugauuaacg ccgaauaccc gucuaccgcg     2880

aucgucagcg ugaaagccaa ucuuacacau caauuuagac agaccuucga cuuuccuaag     2940

auucgggagg ugaacgauua ucaccaugcu uucgacgccg cucugacggc cuuuaucggc     3000

acauaccucc ugaaacgcua ucccaagcug gagagauucu uugucuacgg gaaauucgcc     3060

aagcagccug uuaaccuuac ccgguucaau gugauucgca aacuggcuaa gaccgacaca     3120

cccaucaccg auauugacac aggagaagug cucugggaua agcugacgga caucaaauac     3180

uuugagaccc ucuaugauua caagagacug cuugucacac gggaaguguc cgagaacuau     3240

ggcgcgaugu ucgaccagac ccugcucaaa gccucugaua augggaagag acugauccgc     3300

aaaaagcaga gcuuugacac agcuauuuac ggaggcuaua ccgggaagac gcuggccuac     3360

auggccaucg uuaaaguguc caccaagaaa gauguccaau aucagguggu uggcgugccu     3420

acaaccaugg ucacacaaau ugaacagcuu gagcagcagg gaaugaccaa caagcaagcu     3480

gugacaaaag uuaucgagcc ccugauugcc gcgaauaaca agaagaugcu caccuacgaa     3540

gugcugcucc cgaaaguccg guucgaacag guggugagag accgcaucaa gggccagacg     3600

caccgguuug cucuggggac agauaccuac uaucacaaua uccaggaacu uuaccugccu     3660

cucacccugc aaagagccuu ccugggaaaa cagaacgaga gcgaggucca gcuuaaucag     3720

aaccugcuca caguuuuuga cgccacccug gcucaaguga aucgcuauuu cccccucuac     3780

gaaaugaaca aguuuaaaaa gauucuuaau ucugcccggg aucagaucga gcugcuggaa     3840

gcgaagaacc aguucgugaa aaauaagcug guccucggca aacaagggau ucugacgaac     3900

auguuuaucg gacuucaugc uaacgccucc uucggcgacc ucaagagccu gggggugaaa     3960

acaucuuucg gccagcugca gguucccgga ggcauuaagc ucaccgggga cgccgagauc     4020

aucuaucagu ccccuacagg acuguuugaa agaaagauug cucuuaaaga ucuguga        4077


<210>  4217
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4217
augaagcagg gcaaaagagg guacuaucug ggacucgaca ucggcaccga uagcauuggg       60

uacgccguga cagacgagca auaucaucug cuucgguucc auggaaacga ugcuuggggc      120

ucccacgucu uugacucugc cagccugugc gaugaacgca gaggguuccg guccgcgcgc      180

agacggcucg accgcagaca gcagcggguu aagcugcuuc aggagaucuu ugcuaaagaa      240

auugccaaua ucgauccccg cuucuuuauu agacugucug agagcuaccg guggcgcgac      300

gauacggacg auagauauau cuacuucaac gaagaggagu auaccgacau ccaauacaug      360

cgggauuacc cuacaauuca ucaccucauc ugugaacuga uggagaauga agaggagcau      420

gacauucgcc ugguguaucu cgccugcgcu uggcuggugg cccacagagg acacuuucuu      480

ucccagcugu cugucgacaa caucgauagc cucauugaca ucgaauccgu guaccagaag      540

uuccugcacu uuuucauuga uaauggcugu gagaacccgu ggauccagga cgcugaacuu      600

guuggcggga uccugaaaga gcacauugga gugaccgaaa agcauaaucg gcucggcgag      660

aucaugcugg ggaacagaaa gcccagcaaa gauguccgcg acacauuucu guauucugag      720

uacugcauua uccggcuccu ggccggaggc accuguaaac uuagagaucu guaugggaaa      780

gaagaguacg aagacuucgg auccgugagc cucgagaugg augacgauaa gcuggcggaa      840

auuaugauca auaucggcga ggacuaugag cuuauugccg uucugcgcuc ucuccacgac      900

ugguccgugc uggcugauau ccuggacggc caaaacgggc ucacaaauau uagcaccgcc      960

aaagucgcua ucuacgaaca gcacaagaag gaucuggcca cgcuuaaaua cuuuauucgg     1020

aaguauugcc cugagaaaua caacgcgauc uucaaggacg ccagagaaga uaauuaugug     1080

gcuuacucuu aucauuacaa caauaaacug gacggacaga ucaagaaaaa ggccgauacc     1140

gagacauucu ccaaguuucu cauuaaaauc auucacagca ucaagcccga caaagaagau     1200

gagauuaucu aucaggacau ggauaaccgc cugggccuuc ggcaauuccu gccuaagcag     1260

agaaacaccg acaaucgcgu gauuccccau cagcucuacg aguacgaacu gaaaaagauc     1320

cuggagaacg cugccaagua ucucuccuuu cugaaucaaa aagaagaugg gaucucuaac     1380

acagagaaga uuaucagcau uuucaaauuu aagaucccuu acuaugucgg accgcuuaau     1440

aaacacuccg aguucgccug gauugacaga aagagcggca agauccugcc cuggaacuac     1500

gaaaauauga ucgacgauga cgcuucugag gaagcguuua uuaaaaagau gaccaaccag     1560

uguacguauc ucccugggga guccguucug cccaaagaua gccugugcua ccagaaguuc     1620

auggugcuua augaaaucaa caauauuaaa guggacggac ggaagaucuc ugucggcgcc     1680

aagcagggca uuuauagcga ucucuuucug aaauacaaga aagugaagcg cucccaaauc     1740

gaggaguacc ugaucucuaa cgggcagcuc gaaaagaauc ggagagagac acugagcgga     1800

auugacaucc agauuaaguc cagccuuucu ucccauaucg cuuuccggcg ccugcucgaa     1860

cagaaaauuc ugaacgaggc cgauguugaa agaaucaucg agcgggccag cuaugcugag     1920

gacaagggca gaguggccaa guggcugcgc gcgaaauacc cucaccuuac cgaagcugau     1980

auuaaguaua ucuguaaagu caagauuaaa gacuuugggc ggcucucuag aacauuccug     2040

accggaaucg agggcgccug caaggaaacc ggggaggugg ccacaauuau cuccaugaug     2100

ugggaaagca augauaaccu gauggagcuc cuguccgagc gcuacacguu ugcugacacc     2160

auucuugagu ucaagucuga uuauuacaca gaaagaaaac aaacccugag cgaacggcuc     2220

gacgagaugu auguuuccaa cgccgugcgc agacccaucu accggacacu ggacaucguc     2280

aaggaucugg agaaggcuuu cggcaagccg gagaaaauuu uuaucgaaau gacccgcgga     2340

gccaugccug aacuuaaggg caagagaacg aaaucucggc agcagcagcu ccuggaguac     2400

uaugacaagu guaaagaaga ggaugugcgc gaccugaagc aacagcucga ggcgcugggg     2460

gaauacgucg auaauaaacu ucagagcgac agacuguucc ucuauuuuau gcaguucgga     2520

aagugcgccu acuccggcau gcccauuagc cuggagcggc ugauggcugg gucuaaggaa     2580

uaugauaucg accacauuua cccucaagcc uaugugaaag augacuccau caucaacaau     2640

aagguucuug ugcucagcgu cgcuaacgga gagaaaaagg auguguaccc cauuaaaucu     2700

gaaauccgca auaagaugca gaaaacaugg uccuuuuggc aucacguugg caccauuagc     2760

gacgagaagu acaagagacu gauccggucu acccccuuca cagaggacga aaaauauggg     2820

uuuauuaaca gacagcugac cgagacaagc caauccacga agguggucgc cgaacuccug     2880

aaagagcgcu acccugaagc ggagaucgug uauagcaagg ccggccuuau cucugauuuc     2940

cggcaugagu uugaccugua caaauccaga agcuauaaug aucuccacca cgcuguggac     3000

gccuaccuga acauuguugu cggaaaugug uaucauaugc gcuucucccg gcagugguuu     3060

aacgucaauu cuagcuacuc caucaagacc aagacacugu ucacccacac agugaacugc     3120

aauggcaaag aaguguggaa caaggauaug cuuccggggg uucugaaaac cgcuaagaaa     3180

aauacggccc auuuuaccaa gcacgccaca uucaagaccg gaggccucuu cgaccagaac     3240

cccgugaaaa aggcuccugg gcugacaccc cucaaagcgg gacugccuac cgagaaguac     3300

ggcggguaua acaaagccgg cgucauguuu uucauucccg ugagauacaa gucuggaaag     3360

aaaacgguuc uuauggugcu gagcgucgaa cuccuguaug gcaaucgcuu ucuggaggau     3420

gagaucuucg cuaaggaaua cgccaaagac cggcuucaga gaaugauugg gaaguccgug     3480

gaugagaucg acuuuccgau uggaaugcgc ccuuggaaag uuaacacaau ccugucucuc     3540

gauggcuucc ggaucuguau uaccgggaau gccagcggag gcaagugccu gaucgcucaa     3600

cccauuaugc aguuuuccag cgacgaauau uggaaguacu aucucaaaaa gguggagaaa     3660

uucgucgaaa aggugaaaaa caauucuucc uacguguacg augucgacua ugacgccguu     3720

cagcaugagg auaaccugaa acuuuacgac cuguaucucg auaaacugca gaauagcauc     3780

uacagaaagc ggauuaacgc gccuauccaa acacugaucg aggggcgcga aaaguuuauu     3840

gacuguucug ugaucgagca gugccaggug cuucugaaua uucaccaggu cuucggcaga     3900

augaccuccg gaugugaucu cacccugauc ggcgggaaaa cacggagcgc uucuacggug     3960

aacuuuucca gcauuauccu caauuggaag aaaaacuaua ccgacguucg caucauugau     4020

caauccgcca gcggacugug ggaagugguc ucugagaaua uccuugaaua ccuguga        4077


<210>  4218
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4218
augggcaaga uguacuaucu ggggcucgac aucggaacca acagcguggg cuacgccguc       60

acagaucccu ccuaucaccu ucuuaaauuc aagggggagc cuaugugggg agcucauguu      120

uuugccgcgg gcaaucaguc ugcugaaaga cggagcuucc gcacguccag acggcgccug      180

gacagacggc aacagcgcgu gaaacucgug caggagauuu uugccccggu caucucuccc      240

auugauccua gauucuuuau ccggcugcac gaaagcgccc uuuggcgcga cgauguggcu      300

gagaccgaca agcauauuuu cuuuaacgau cccacauaca ccgacaaaga auauuacucc      360

gauuauccua caauccacca ucugaucguu gaccucaugg agucuagcga gaagcacgau      420

cccagacugg uguaccuggc cgucgcuugg cucguggccc accgggggca uuuccugaau      480

gaaguugaca aggauaacau uggagacgug cuuuccuuug acgcguucua cccugaguuu      540

cuggccuucc ucagcgauaa uggcgucucu ccgugggugu gcgaauccaa agcucugcag      600

gccacccuuc ugagccgcaa cucugugaau gacaaguaua aagcucucaa gucccugauc      660

uuuggcagcc aaaaacccga ggauaacuuc gacgccaaua uuucugagga ugggcugauc      720

cagcuccugg cgggaaagaa ggucaaaguu aacaagcugu ucccucagga auccaaugac      780

gccagcuuua cacugaacga uaaagaggac gcuauugaag agauccucgg cacccugacg      840

cccgaugaau gugaguggau ugcccacauc agacggcuuu ucgacugggc uaucaugaag      900

caugcccuga aagaugggcg caccauuucc gagucuaagg ugaaacucua cgaacagcac      960

caccaugacc ugacacaacu gaaguauuuu gugaagaccu accucgccaa agaguaugac     1020

gauaucuuca gaaaugucga cagcgaaaca accaagaacu acguggcuua uuccuaccac     1080

guuaaagagg ugaagggaac gcugccuaaa aauaaggcga cacaggaaga guuuugcaag     1140

uacguccuug gcaaagugaa gaacauugag uguagcgaag ccgauaaagu ugacuucgau     1200

gagaugaucc agcggcugac cgacaacucu uuuaugccca aacagguguc cggggaaaau     1260

agagucauuc cguaucaacu cuacuauuac gagcugaaga caauccuuaa caaggcugcc     1320

agcuaucugc cuuuccucac ccagugcgga aaggaugcca ucucuaauca ggacaaacug     1380

cugagcauua ugaccuuucg cauccccuac uucgugggcc cucuccggaa ggauaacucc     1440

gaacaugcuu ggcuggagag aaaagccggc aagauuuauc ccuggaauuu uaacgacaaa     1500

gucgaucuug acaagucuga ggaagcguuc auccgccgga ugacaaauac guguaccuac     1560

uaccccgggg aggaugugcu gccucucgac agccugauuu augaaaaguu uaugauccug     1620

aacgagauca auaacauuag aaucgacgga uacccgauuu ccguugaugu gaaacaacag     1680

gucuucggcc uuuucgagaa gaaacgccgg gugacaguua aggacaucca gaaucuccug     1740

cugagccucg gggcucugga uaaacacgga aaacuuaccg gcauugacac aaccauccau     1800

ucuaacuaua auacguacca ccacuuuaag ucccugaugg aaagaggggu gcucacacgc     1860

gaugacgucg agcggauugu ggaaagaaug accuauagcg augacaccaa gcgcgugcgg     1920

cuguggcuga acaauaacua cggaacacuu accgccgaug acguuaagca uaucucuaga     1980

cuccggaaac acgauuucgg ccgccugucc aagauguuuc ugacaggccu caaagggguc     2040

cauaaggaga cgggagaaag agccagcauc cuggacuuca uguggaacac caaugauaac     2100

cuuaugcagc ugcucuccga gugcuauaca uuuucugacg agauuaccaa acugcaagag     2160

gcuuacuacg ccaaggcuca gcugagccuu aaugacuucc ucgauuccau guauaucucu     2220

aacgccguga agcggcccau uuacagaaca cuggcggugg ucaaugacau ccgcaaagcc     2280

uguggcaccg cuccuaagcg gauuuuuauc gagauggcca gagaugggga aagcaaaaag     2340

aaacgcuccg ugacgagacg ggagcagauc aagaaccugu aucgcagcau uagaaaggac     2400

uuccagcaag aaguugauuu ucucgagaaa auccuggaga auaagucuga cggacagcuu     2460

caguccgaug cucuguaccu cuauuucgcc cagcugggcc gggacaugua caccggggau     2520

cccauuaaac uggaacacau caaggaccag agcuuuuaua acauugauca caucuacccu     2580

caaucuaugg ugaaagacga cucccuugau aauaaggucc ucgugcagag cgagaucaac     2640

ggcgaaaagu cuagccgcua cccccuggac gcggccauua gaaauaaaau gaagccgcug     2700

ugggaugcuu auuacaacca uggacucauc ucccugaaaa aguaucagcg gcuuacacgc     2760

agcaccccuu ucacagacga ugagaaaugg gacuuuauua auagacaacu gguugaaacc     2820

cggcagucua cgaaggcccu cgcuauccug cugaaacgca aguuccccga uacagagauu     2880

guguacucca aggccggccu uagcuccgac uucagacacg aguuugggcu ggucaaaucu     2940

cggaacauca augaucucca ucacgccaag gacgcuuucc uggcgaucgu gaccggaaac     3000

guuuaucaug aaagauuuaa ccgccggugg uucaugguga aucagccuua cagcgucaaa     3060

acaaagaccc ucuuuaccca cuccauuaaa aacggcaauu ucguggccug gaacggggag     3120

gaagaucugg gaagaaucgu gaagaugcuu aagcaaaaua aaaacacaau ucacuuuacg     3180

cgcuucucuu uugaccggaa ggagggccug uucgauaucc agccccucaa agcuagcacc     3240

gggcuggucc cuagaaaggc cggccuggac guugugaaau auggaggcua cgacaagucc     3300

acagccgcuu acuaucuucu ggugcgcuuu acccucgagg auaagaaaac acagcauaag     3360

cugaugauga uucccgucga agggcucuac aaagcccgga ucgaccacga uaaggaguuc     3420

cugaccgacu augcgcagac gacaauuucu gaaauccuuc aaaaagauaa gcagaaggug     3480

aucaauauua uguucccgau gggaaccaga cauaucaaac ugaacagcau gauuuccauc     3540

gacggcuuuu accucagcau ugggggaaag ucuuccaaag gcaagagcgu ucugugccac     3600

gcuauggugc cucugaucgu cccccauaaa aucgaguguu auauuaaggc cauggaaucu     3660

uucgcccgca aguuuaaaga gaauaacaaa cuucggaucg uggagaaguu cgauaagauu     3720

accguugaag acaaucugaa ccucuacgag cuguuucucc agaaacugca gcacaauccc     3780

uauaacaagu ucuuuuccac acaauucgau gugcuuacca augggagaag cacauuuacg     3840

aaacugucuc cugaagaaca gguccagacc cugcucaaca uccuguccau uuucaagaca     3900

ugccggagcu ccggcuguga ccuuaagagc aucaacggau cugcucaggc cgcgcgcauc     3960

augauuagcg cugaucugac cggccucucc aaaaaguacu cugacaucag acugguggaa     4020

caaagcgccu ccgggcucuu uguuucuaaa agccagaauc ugcuugagua ccuguga        4077


<210>  4219
<211>  4083
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4219
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uuggcagcaa cuccaucggg       60

uucgccgcug ucgaugaaaa uucucaccuc auuagacuga agggaaaaac aguuaucggc      120

gcccggcuuu uugaagaggg gaaggcggcu gccgaccgca gagccagccg gacgacccgc      180

agacggcugu cccgcaacag auggcggcuc ucuuuccugc gcgauuucuu cgaaagccau      240

auuacaccua ccgacccgaa cuuuuucaug agacagaaau auuccgagau cucucccaag      300

gauaaggcuc gguacaaaua ugaaaagcgc cuuuuuaaug acagaacaga ugccgaguuc      360

uaccaacagu auccuaccau guaccaucug cggacacgcc uccugaccga ccccagcaaa      420

gcugauguga gagagaucua uuuugccauu caccacaucc ugaagucccg gggacauuuc      480

cucacgagcg gcuacgcgaa agacuuuaau accaacaagg uggcccugaa ugaaauuuuc      540

ccugcccuuc aggaugcuua cgcucagguc uaucccgacc uggauaucac auuugacgag      600

aacaagguga augaauuuaa aaccguucuc cuggacgaga aggccacacc uucugauacc      660

caaagagcgc uugugaaccu gcuccuggcc gaggacggcg auaaagacau ucugaagcag      720

cagaaacagg uccucacgga guucgcuaag gccgugguug ggcugaaaac aaaacuuaau      780

guggcucugg gaaccgaggu cgauuccagc gaagccacag ccuggaacuu uucucucggc      840

caacuggacg auaagugggc ugggaucgag uccgcgauga ccgacgaagg aaccgagauu      900

cuugaucaga uccgcgaccu guaccgggcc agacuccuga auggcaucgu gccggcuggg      960

aaaacacuga gccaggccaa gguggaugac uaugagcagc accacgaaga ccucgagcug     1020

uucaaagauu accuuaagca acuggaagac gauggaaugg ccaaagcuau ucgccagcuc     1080

uaugaccggu acaucgaugg cgacgaugcc aagcccuuug ucagagagga cuucguuaag     1140

gcgcugacga aagaagugac cgcucauccu aacgccaagu cucccgagcu ucuggagcgc     1200

cucgugcagc cugauuuuau gcugaaacaa cggaauaagg ccaacggcgc uauucccguc     1260

cagaugcagc agagagaacu ggaccaaauc auuaaaaacc aguccgugua uuacgauugg     1320

cucgccgcuc cgaauccugu ugagaagcac cgccagagca ugcccuacca guucgacgaa     1380

cugcuuaacu uucggauccc uuauuacgug gggccccugg ucacagccaa ggagcaaaaa     1440

gcggcccagg gaggcguguu cgcuuggaug guuagaaagg aucccgacgg gaauaucacc     1500

ccuuauaacu uugacgaaaa aguggaucgc gaggccuccg cuaauacauu cauucagcgg     1560

augaagacca cggacacaua ccucaucgga gaggaugucc ugccgaaaca gucucugcuu     1620

uaucaaagcu acgaagugcu caacgagcug aauaacguca gagugaauga caacaagcug     1680

uccuuugauc agaagcagca gguucucggc gaacuguucg agcggcauaa uaccgugacc     1740

auuaaacaau uugucgacaa ccuucuggcc cacggggauu augaccgcau ccccgaggug     1800

agaggacucg cggaugaaaa gcgguuccug agcucucugu ccacauacaa ucagcuuaaa     1860

ggcauucucc acgaggccau cgacgauagc gacaagcaga ccgauaucga aaagauuauc     1920

gcuuggucua caauuuucga ggacaaggcc aucuuuaaaa cgaagcugaa cgaaauuagc     1980

uggcugaaug cuggcaugau caacucccuc gccaacauua gauaccgcgg guggggacaa     2040

uucucucaua agcugcuuaa ugagcugacc cucagcacag gccggaccgu uuuugaggaa     2100

cugcugaucu ccagccacaa ccuuaugcag auccucacag acgaggugcu gaaacagaag     2160

auugccgaac ugaaugcuca ugagcucaaa gcgucuggga ucuaugccgc uauugaugac     2220

gccuacaccu ccccuagcaa caagaaagcc cugagacagg uccuuucugu gaucgaugac     2280

auuacgaaug cugccggaca agcgcccaac uggcuguaua ucgaaaccgc ugauggcgac     2340

ggggugccug gcaagcgcac aaauuccaga cagcggaaga uccaggagau uuacguuaac     2400

gccgcccagg agcucaucac cggaagcguc cgcggcgaac uggaggauaa aauugcuucc     2460

agagccgacu ucacagaucg gcuggugcuu uauuuuaugc aagggggacg cgacaucuac     2520

accggcgaac cccucaauau ugauaaccug ucuagcuaug acaucgacca cauccugccg     2580

cagucccuca uuaaggauaa uucucuggac aacagagugc uuguccaucc ucuggugaau     2640

cgggagaaag auacgaucuu cgcgagcaca cgcuuugcua agcagaugaa cgggaugugg     2700

agacaguggc acgccgccgg acucauuucc agccggaaac ugcgcaaucu gcaaaugaga     2760

cccgacgaaa ucgauaagua cgcuucuggc uucguugccc ggcagcuugu ggagaccaga     2820

cagauuauca agcugacaga gcaaaucguc gcugaccagu acccugaugc caaaauuauc     2880

gcggugaagg ccgggcucuc cagccagcug cgcaaagaac ucgacuuucc caagaaccgg     2940

gagguuaacc acuaucauca cgcuuucgau gccuuucugg cugccagaau uggcaccuac     3000

cuucugaaac gcuauccuaa ucucgaaccc uucuuuaccu acggaaaguu caagaaaaca     3060

gaggugaaga aacugaaguc uuucaacuuu auccgggaca ugacgcaugc caaagauaag     3120

auugucgcua aagagaccgg cgaaaucgug ugggacaaug cguccgauau uaacgagcug     3180

gacagaaucu auaauuucaa gcgcaugcuu aucacacacg aaguucgguu ugagaccgcc     3240

agccuguuca agcagacacu cuacgcugcc aaaaacucua agaauagagg gggaagccgc     3300

caacugauuc cgaaaaagaa aggcuaucuc guggacaucu acgggggaua cacccaggaa     3360

acgggcuccu aucugagcgu cgugcggcuu acaaagaagg ccauguacgc uguggucaaa     3420

guuucuacca gagaugccgc gaagcuggcu guggccaaau ccauuagcga gcagaaggag     3480

aacgaaaccc ucaaaaagau cauugacggg aagcugucca aaacaucuaa gaaaggcaag     3540

accacacacc agcuguuuga gaucgugcuu ccucgggucg gacagaaaac gcuguucaag     3600

aauagcaagu auaaccaauu ucucguuaau uccgauaccu acaugcauaa cuaucaggaa     3660

cuguggaugc cccgcgagua ccagagaaug uggaaagaca uccuccuguc uaaucacggc     3720

gaugcccaac uugaagggca gcuggaccag auuuucaagu uuaucgugag ccaggugaac     3780

uccuauuuca aucuguacga uauuaaccaa uuucggaaaa agaucgcuga cgccacagau     3840

aaauucgcga agcuccccac ccaugacaca gauaacgcuc gcggaaagau ugccaccauc     3900

ggccagcugc uuaucgggcu gcaggccaau gcuaugagau cugaccuccg gaaccuggau     3960

auuaaaacgc cuuucggacu ccugcaaauc ggcaccggga uuacacuuga ccuggacacc     4020

agcaucauuu accagucccc gacaggccug uuugagagag aggucccccu caccgaucug     4080

uga                                                                   4083


<210>  4220
<211>  4083
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4220
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcuc cucuaucggc       60

uucgccgugg ucgaugcuaa uagccaucug cuuagacuga aaggggagac agccauugga      120

gcgcggcucu uucgcgaagg cgaguccgcu gccgggagac gggccucucg cacgaccaga      180

cggcgccuga gccacaacag auggcggcuu cgcuuccugc aagacuuuuu cgauccucac      240

aucuccucua uugacccgga cuuuuuccuc agacagaagu auagcgaaau cucccccaaa      300

gauaaggagc gguuuaaaua cgaaaagcgc cuguucaaug auagaacaga cgcucaguuu      360

uauaagcagu acccuaccau guaucaucug cggcuccacc ugaugaacga ugccagcaaa      420

gcugaccccc gcgagaucuu cuuugccauu caucacaucc uuaagucuag aggacacuuc      480

cugacaccug gcgaugcgaa aaauuuuaac accgacgaug uugaccucaa gcauauuuuc      540

cccgcccuga cagaggcuua cgcccaggcu uauccugacc uuacccuguc cuucgaaccg      600

agcaaagccg agaaguuuaa gucuauccuc cuggaugaag acgcgacguc caaugauacc      660

caaaaagccc uggugaagcu ccugcuuagc gacgauggcg acaaagaggu gcugaagcag      720

cagaaaaagg uccucacaga guucgcuaaa gccguggcug ggcugaaggu uaaguuuaac      780

guggcccuug gaauugaagu caccgaugcc gacguggaga aauggcacuu cggcauggcu      840

caacuggaag auaagugguc ugacaucgag cccucccuca cagaucaggg gauggaaauu      900

uuugagcaga uccaggaccu guaccgggcg agacugcuca auggaaucgu uccugccggc      960

aaaacccuga gccaagcuaa gguggacgau uacgaccagc auugcaaaga ucuuaagcug     1020

uucaaggagu aucucaaaca gcugaacgac cacgaucuug ccaagcgcau ucggcugcuc     1080

uacgacagau auaucgaugg ggacgaugcc aaacccuuuc ugcgcgacga uuucgucaag     1140

gcucugaaaa aggaagugac ggcccacccu acagagcucg cggaaucccu gcuugagcgg     1200

auggaacaag acaauuuuau gcugaagcag agaaccaaag cuaacggauc uauucccgug     1260

cagaugcagc aacgcgagcu cgauaagauc auugccaauc aggccgagua cuauagcugg     1320

cuggcugccg aaaacccggu caaagagcau cgggacucca agccuuacca gcuggacgaa     1380

cuucucaauu ucagaauccc cuauuacguu ggcccuauga ucacaaagaa gcagcaagag     1440

aaaagcggcc agaacguguu ugcuuggaug gugcgcaaag aucccucugg gaacauuacc     1500

cccuacaauu ucgacgaaaa ggucgaucgg gaggccuccg cgaacgccuu uauccagaga     1560

augaaaacca cagacacgua ucugauugga gaggaugugc ugccuaagca gagccuccug     1620

uaccaaaaau augaaguucu uaaugagcug aacaaugugc gcgucaacgg cgaaucucuc     1680

agcaccgacc agaagaaacg gcugaucaga gagcuguucg agcggcacuc cucugugaca     1740

cuuaagcagu ucguugaaaa ucucguggcu cauggggaug ucgccaagcg cccggacgug     1800

agaggacugg cugaugagaa acgguuucug agcucccuca gcaccuacca ccagcugaga     1860

ggcauucuuc augacgccau cgaugaccgc gggaagcaau cugauaucga aaacauuauc     1920

acauggucca ccgucuucga ggacgcgagc auuuuuaaau cuaagcuggg agaaaucucc     1980

uggcucgaca gcaaaacgcg gacaacccug uccaauauua gauaucgcgg cuggggccag     2040

uucucuagaa agcugcuuga ugggcucaag cugggaaacg gccacaccau cauucaggag     2100

cugaugcuca gcucccggaa ucugaugcaa auccuuacag acgagacccu gaaaaaggaa     2160

auggccacac ucaaccagga gcgccugaaa cccgauucua ucaaugaagc uauugacgcc     2220

gcuuacacga gcccuuccaa caagaaagcc aucagacagg ugcugaaggu uguggaugac     2280

auuaagcacg ccgcuaauga ucaggcgccc agcuggcuuu auaucgagac cgccgacggg     2340

ggaggccauc cugggaaacg gacacucucu cgccaaaagc agauucaggc uaucuacgaa     2400

aacaccgcca aagagcugau cgauuccgcc gucagaggcg agcugaagga caaaauugcu     2460

gauaagacaa acuuuagcga ccggcucgug cuguacuuca ugcagggagg ccacgacauc     2520

uauaccggga cggcccuuaa uauugaucaa cugucuuccu acgacaucga ucauauucuc     2580

ccccagagcc ugaucaagga cgauucucug gacaaccgcg uucuugugca cgcgaaaauc     2640

aauagagaaa agaacgcuga guuugccagc acccuguucg ccgcuaaaau ggccuccaca     2700

uggcaccggu ggcgcgaugc gggacucauu agcggcagaa agcugaaaaa ucuccugaug     2760

cggccggacg aagucgauaa guaugcuaaa ggguuugugg ccagacagcu uguggagacc     2820

cgccagauca uuaagcugac agaacaaauc guugccgcuc aguacccuga caccaagauu     2880

aucgccguca aagcuggacu cucucaucag cugcggcaag agcuggauuu ccccaagaac     2940

agagacguga aucacuauca ucacgccuuu gaugcguucc uugccgcucg caucggcacg     3000

uaccugcuca aacgguaucc uaaccuggcc cccuuuuuca cauacgggga guucaccaag     3060

acaaaucuca aaaagcugca guccuuuaac uucauuggcg cucuuaccca uaccgacgga     3120

aagaucacaa gcaaagacac gggcgaagug cugugggaua aucagagaga cauugaggaa     3180

cucgaucaga ucuacaacuu uaagcgcaug cugauuaccc acgaggucua uuucgagaca     3240

gccgggcugu uuaaacaaac cguguacgcc gcuaaggacu ccaaagaacg gggaggcucu     3300

agacagcuua ucccuaagaa gaaaggguau agcacaggaa uuuacggcgg guauacccag     3360

gagacgggcu ccuacaaugc gcugguucgc cucacaaagg ccggaaaagu ggucuaucgg     3420

guguucaaga ucucuaccca gaacgcugcc gaaaucgccg cugccagaag ccuguccgcg     3480

gaggaaggca augagcggcu cugucaaauu cuggagcagu cucuucugaa aaccaacaag     3540

gauaagaaaa acacaguuga ccuguuugau guggucaucc cccgccucgg gaaggaagcu     3600

cuguuccaca auagcaaaua cggacuuuuu cuggugaacu ccgacaccua cuaucauaau     3660

uaccaggagc ucuggcugag cagagaaaac cagaagauuc ucaaaaagcu guucucuaca     3720

aaguaugauc aauccgagau ggaccgggau gcccuucacc uguuugaaac gaucgccaaa     3780

cagguugagc aguucuucaa gcuguacgac auuaaucagu uucgcaccaa aaucagcucu     3840

gcuuucgaug ccuuugagaa gcucccggug gaaggcgagg aagggaaagu cgagacacug     3900

aagcaacuuc ugaucggacu ccaugcgaac ccugugagau ccgaccugaa aaaucucggc     3960

auuaagaccc ccuucggguu ucugcaggcu ggcaagggaa uccaccuuga cgaugacaca     4020

gaaauuaucc agcaaagccc cgccggccug uucaaacgga gagugcgccu ggccgaucuc     4080

uga                                                                   4083


<210>  4221
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4221
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uuggcagcaa cuccaucggg       60

uucgccgcug ucgaugaaaa uucucaccuc auuagacuga agggaaaaac aguuaucggc      120

gcccggcuuu uugaagaggg gaaggcggcu gccgaccgca gagccagccg gacgacccgc      180

agacggcugu cccgcaacag auggcggcuc ucuuuccugc gcgauuucuu cgaaagccau      240

auuacaccua ccggaccgaa cuuuuucaug agacagaaau auuccgagau cucucccaag      300

gacaaggcuc gguacaaaua ugaaaagcgc cuuuuuaaug auagaacaga cgccgaguuc      360

uaccaacagu auccuaccau guaccaucug cggaaucgcc uccugacaga ucccagcaaa      420

gcugacguga gagagaucua uuuugccauu caccacaucc ugaagucccg gggccauuuc      480

cucaccagcg gcuacgcgaa agauuuuaac acgaauaagg uggcccugaa cgaaauuuuc      540

ccugcccuuc aggacgcuua cgcucagguc uaucccgauc uggacaucac cuuugaugag      600

aauaagguga acgaauuuaa aacaguucuc cugaaugaga aggccacccc uucugacaca      660

caaagagcgc uugugaaccu gcuccuggcc gaggacgggg auaaagacau ucugaagcag      720

cagaaacagg uccucaccga guucgcuaag gccgugguug gacugaaaac gaagcugaau      780

guggcucugg gcacagaggu cgauuccagc gaagccaccg uguggaacuu uucucucggg      840

caacuggacg auaagugggc cggaaucgag uccgcuauga cagacgaagg caccgagauu      900

cuugaucaga uccgcgaccu guaccgggcg agacuccuga augggaucgu gccggccgga      960

aaaacccuga gccaggcuaa ggucgaugac uaugcccagc accacgagga ucucgaacug     1020

uucaaagacu accuuaagca acuggaggac gauggcacag ccaaagcuau ucgccagcuc     1080

uaugaccggu acaucgaugg cgacgaugcc aagcccuuug uuagagaaga cuucgugaag     1140

gcgcugacga aagaggugac cgcucauccu aacgccaagu cucccgaacu ucuggagcgc     1200

cucgcccagc cugauuuuau gcugaaacaa cggaacaagg cuaauggggc cauucccguc     1260

cagaugcagc agagagagcu ggaccaaauc auuaaaaacc aguccgugua uuacgauugg     1320

cucgcugccc cgaauccugu ugaaaagcac cgcaagagca ugcccuacca gcuggacgag     1380

cuucugaacu uccggauccc uuauuacgug ggaccccucg ucacagcgaa agaacagaag     1440

gccgcucaag gcgggguguu ugccuggaug guuagaaaag aucccgacgg aaauaucacc     1500

ccuuauaacu ucgaugagaa gguggaccgc gaggcuuccg ccaauacauu uauucagcgg     1560

augaaaacca cggacacaua ccugaucggc gaggaugucc ugccgaagca gucucuucuc     1620

uaucagagau acgaggugcu gaacgaacug aauaacgucc gggugaauaa cgagaagcuc     1680

agcauugaac aaaaacagca gguuauccgc gagcuguucg agagacauaa uuccgugacc     1740

auuaagcagu uugcggaaaa ccuucgggcc cacggggacu augcucacau ccccgagauc     1800

agaggacugg ccgaugaaaa acgcuuccuc agcucucugu ccaccuaccg gcaacuuaag     1860

agccuucucc cugacgcuau ugaugacccc gccaaacagg ccgauaucga gaauauuauc     1920

gcuuggucua cagucuuuga agacgcggcc auuuucaaga ccaaacugaa ggagaucaac     1980

uggcuggaua gccagacaau uacgaagcuc uccaacauca gauaccgcgg cuggggccaa     2040

uucucucaua aauuucugaa ugggcuuacc cugggaaacg gccacacaau cauucaggaa     2100

cucuuccuga gcaauaacaa ucugaugcag auccuuaccg acgaaacacu ccagaagaaa     2160

augaccgagc ugaacgcuga uaagcugaaa gccgccaaua uuaacgacgc uaucgaugac     2220

gccuauacgu ccccuagcaa uaagaaggcg cucagacaag ugcugcgggu uauugaagac     2280

aucaaacgcg cugccgaugg gcaggacccc ucuuggcuuu acguggagac cgccgaugga     2340

ggcgggacac cgggcaagag aacccgggcu cgccagcauc agcugcaaga aaucuaugcc     2400

aacgcggcuc acgagcucau ugacuccgcc gucagaggag agcuggaaga uaaaaucgcc     2460

gacaaggcug auuuuaauga ccggcuggug cuuuacuuca ugcagggcgg gcgcgauauu     2520

uauacaggaa ccccucucaa caucgaccag cugagcucuu acgauauuga ccauauccug     2580

ccccaguccc ucaucaaaga caauagccug gauaacagag ugcuuguuca cgccauuauc     2640

aaucgggaga agaacgcuac guuugccucc acaauuuaug cgcaaaagau gaacgccacc     2700

uggcgccagu ggcacgaagc uggccugauc ucugggagaa aacuccggaa ucugcagaug     2760

agaccugacc aaauugauaa guacgccagc ggauucgucg cucgccagcu gguggagaca     2820

cggcagauca ucaaacuuac cgaacagauu guggccgccc aauaccccga gaccaagauc     2880

auugcuguca aagcgggccu gucccaucag cucagagagg aacuggaguu uccuaagaac     2940

cgcgacguga aucacuauca ucacgccuuc gaugcuuuuc ucgccgcccg gaucgggaca     3000

uaccugcuua agagauuccc caaccugcag gccuuuuuca cguauggcaa auuuaagaaa     3060

gcugacguua agaaacuccg cggauucaau uucauucggg auaucaccca cgcggaagac     3120

aagauugugg ucaaagauac aggcgaggug aucugggaca agaagagaga uguugacgag     3180

cuggaucgca ucuacaacuu uaaacggaug cugauuaccc augaagugca cuucgagaag     3240

gcugaccuuu uuaaacagac agucuauggg gccaaggacu cuaaagaagc cggaggcaag     3300

aagcaacuga ucagcaaaaa gaaaggguac ccgguggaua uuuauggagg cuacacccag     3360

gagacggggu ccuaccucag cguugugaga cugacaaaga aagcuaugua ugccgucgug     3420

aaggugucua cccgggacgc ggcuaagcuc gccgucgcca aauccaucag cgaacagaag     3480

gagaaugaga cccugaacga uauuaucgac gaaaaacuuu cuaagaucuc caaaaauggc     3540

aagauuacac augagcuguu cgaaguugug cucccucgcg ugggacagaa gacccuguuu     3600

aaaaacagca aguacaaucu guuccuuguc aacucugaua cauauaugca caauuaccaa     3660

gagcugugga ugcccagaga auaucagaaa auguggaagg acaucagccu cuccaaauac     3720

ggcgaugcuc agacggagga ccagcuggau caaauuuuug gguucaucgu gagccagguu     3780

aacucuuauu uuaaucucua cgacauuaac caguuccgga agaagcugaa cgaugccgcu     3840

gacaaauuug ccacccuucc caugcgcgau acagacgaca ugcaaggaaa gaucgcgacc     3900

aucggccagc ugcugauugg gcuccaggcc aaugcugcca gauccgaucu gcggaaccuu     3960

gacaucaaaa caccucuggg acuccugcag auuccgaaug gcaucagccu cgauuccgac     4020

accucugugg ucuaccaaag ccccacgggg cugcuugaga gagaagugca ucuguccgau     4080

cuguga                                                                4086


<210>  4222
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4222
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg auggucuguc       60

guuacagaug acuauaaggu gccugccaaa aagaugaaag ugcugggcaa uacggauaag      120

gaguacauca agaaaaaccu gauuggggcc cuucuguucg acagcggaga aaccgcugag      180

gcgacaagaa ugaagcggac cgcucgcaga cgguauacac gcagacggaa ucgcauccuc      240

uaccugcagg auauuuucuc cccggaacuu aaccaagucg acgagucuuu ccugcacaga      300

cucgaugaca gcuuucuggu ggccgaagau aaacggggcg agcgccaugu uaucuucggg      360

aauaucgccg acgaggugaa guaucacaaa gaauuuccca ccauuuacca ucugagaaag      420

caccucgcug auuccucuga gaaggccgac cugcggcuug ucuaucuggc ucucgcccau      480

aucauuaaau accgcggaca cuuccugauc gaugaaccua uugacaucag aaacaugaau      540

agccagaacc uuuuuaagga guuccugcuc gcguuugacg gcauucaggu ggauugcuac      600

cuggccucca aacacacaga caucagcggc aucauuaccg cuaagaucuc uaaaucccgg      660

aagguugagg ccgugcugga acaguucccc gaucaaaaga agaauagcuu uuucgggaac      720

auggucucuc ucguguuugg acugaugccu aauuucaagu ccaacuucga gcuugacgaa      780

gaugcuaaac ugcaguuuag ccgcgacucu uaugaugagg accucgaaaa ucugcuuggc      840

auuaucgggg augaguacgc cgacguguuc gucgcggcca agaaaguuua ugauuccauu      900

cugcucagcg gaauccugac gaccaacaau cauuccacaa aggcuccccu gucugccagc      960

augaucgaca gauacgacga gcacaacucc gauaaaaagc uccugcggga cuuuauuaga     1020

accaacaucg gcaaggaagu guucaaagag guguuuuaug auacaagcaa gaauggguac     1080

gcuggauaua uugacggcaa aaccaaccag gaagauuucu acaaguaccu uaaaaaucug     1140

cuccagaagg ucgacggcgg ggauuauuuu aucgagaaga uugaacgcga ggacuuccug     1200

cggaaacaaa gaacguuuga uaacggaucu aucccucauc aggugcaccu ugacgagaug     1260

aaggccaucc ugcgccggca gggcgaauuu uacccguuuc ucaaagagaa ugccgaaaag     1320

auucagcaaa uccugacauu caaaauuccc uauuacguug ggccucuggc uagaggaaac     1380

ucccgcuuug cgugggccag cuauaauucu aacgagaaga ugacccccug gaauuucgau     1440

aacgugaucg acaagacaag cuccgcucag gccuucauug aacggaugac caauaacgau     1500

cucuaccugc cugaccagaa aguccuuccc aagcacucuc ugcucuauca aaaauuugcc     1560

guguacaaug agcugaccaa gaucaaauac guuacagaga cgggcgaagc uagacuguuc     1620

gacguguuuc uuaagaaaga gauuuucgau gggcucuuua agaaggaacg caaagucacc     1680

aagaaaaaga uccugaacuu ccuggacaaa aauuuugaug aguuccggau cacagacauu     1740

cagggacucg auaacgagac cggcaacuuu aaugccagcu auggcacaua ccaugaccug     1800

cuuaagauca uuggggauaa ggaguucaug gacuccagcg auaacgugga cguccuggaa     1860

gauaucgugc ucucucugac ccuguuugaa gacagagaga ugauuaaaca gcgccuucuc     1920

aaguaugaag auaucuucuc caaaaagguu aucgcgaauc ugacgcggag acacuacaca     1980

ggauggggcc ggcugagcgc uaaacucauu aacgggauca aggacaagca uucucgcaaa     2040

accauucugg acuaucuuau cgaugacgga cacuccaaua gaaacuuuau gcagcugauu     2100

aaugaugaca accucagcuu caaggaugag aucgccaauu cccaagugau cggcgacggg     2160

gaugaccugc aucaggucgu gcaggagcug gccggcucuc cggcuauuaa aaagggaauc     2220

cuucagagcc ucaaaauugu ugaugaacug gugaagguca ugggcuacaa cccugagcaa     2280

aucguggugg aaauggcccg ggagaaucag accacagcua gagggcgcaa caauucccag     2340

cagcggcugg gaucucucac caaggccauu caagacuucg gcagcgauau ccugaaaaga     2400

uaucccguug aaaacaauca gcuucagaac gaccagcugu accucuacua ucugcaaaac     2460

gggaaggaca uguacacagg agauacgcug gacauccaca aucuuuccca guaugauauu     2520

gaccacauca uuccucagag cuuuaucaaa gauaacucuc ucgacaaucg cguccugacc     2580

aacuccaaga gcaauagagg caaaucugau aacgugcccu ccaaugaggu ggucaagcgg     2640

augaaagggu ucuggcugaa gcaacucgac gcgaagcuga uuagccagcg caaauuugau     2700

aaccuuacaa aggccgagag aggcggacug ucugcugaag acaaagccgg cuucaucaag     2760

cggcagcucg uggagacccg ccagauuaca aaacauguug cuagaauccu ggaugaacgg     2820

uuuaaucgcg acuucgauaa gaacgacaag agaauccgga augugaaaau ugucacccuu     2880

aagagcaacc ugguguccaa uuuucgcaaa gaguucgggu uuuacaaggu uagagaaauc     2940

aacaauuucc accaugccca cgacgcguau cugaacgccg uggucgcuaa agcccuccug     3000

auucgguacc ccaagcucga gccugaguuu guguauggag aauacccgaa guacaacagc     3060

uauagagagc gcaaaucugc uacggaaaag auguucuucu acuccaauau caugaacaug     3120

uuuaaaacca caauuaagcu ggccgauggc cggguugugg agaaacccgu caucgaggcc     3180

aaugaagaga ccggggaaau cgcuugggac aagacaaagc acuucgcgaa cgugaaaaag     3240

gugcuuagcu auccucaagu cuccauuguu aaaaaggugg aggaacagac cggaggcuuu     3300

ucuaaagaga gcauccugcc caaagggggc uccgauaagc ucauugccag aaaaacgaag     3360

aauaacuacc ugucuacaca gaaauaugga ggcuucgaca gcccuaccgu ggcuuacucc     3420

aucauguuug ucgccgauau ugagaagggg aaaucuaagc gccugaaaac agugaaggaa     3480

augaucggaa ucaccauuau ggagcggagc agauucgaau ccaauagcgu uaccuuucuu     3540

gaggaaaagg gcuaucgcaa cauccgggag aauacaauua ucaaauuccc caaguacucu     3600

cuguuugagc ucgaaaacgg gagacgccgg cugcucgccu ccgcuauuga gcugcagaaa     3660

ggaaaugaaa uguuccuucc gcaacaguuu gugaaccugc ucuaccaugc ccagcacgcg     3720

aauaaggagg acagcgucau cuaucuggaa aaacauagac acgagcuguc ugagcuuuuc     3780

caucacauua ucggcguguc cgaaaagacg auccugaagc cuaaaguuga gaugacccuc     3840

aacgaggcuu uugaaaagca cuucgaguuc gaugaaguga gcgagcuggc ccagucuuuu     3900

auuucccucc ugaaauucac agccuuuggg gcucccggcg gauucaaguu ucuugacgcc     3960

gauaucaaac aaagcaaucu gcgguaccag accgucacag aagugcuguc cagcacccuc     4020

auucaucagu cuguuacggg ccuguaugag acacgcaucg accuuagcaa gcucggggga     4080

gaauga                                                                4086


<210>  4223
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4223
augggcaacu acuaucuggg gcucgacauc ggaaccaaua gcgugggcug ggccgucaca       60

gaugagaacu acaauauuga aagauucaag aaaaagaaca ugugggggau ccggcuguuu      120

gacgaggcua aaacggccga aucccgccgg auucacagac aggcgcgcag aaugcggcaa      180

cgcaagaucc agagacuuaa ucugcucaug gagcuguucg auguugaaau uucuaaagug      240

gacgagagcu uuuucauccg gaagaaggag uccgaucuuc auuaugaaga caaaucucag      300

gauagccgcu acgcucuguu uaacgacaag gaguucaaug auaaagacua ucacaacaag      360

uaccccacca ucuuucaucu cauuauggau cugaucgaaa augacgagaa aaaggauauu      420

agacugcucu aucuggccug ccacuaccuu aucaagaacc ggggacauuu cauuuuugag      480

ggccagaaau ucgacgucaa uuccucucug aacaauaaca ucaauaagcu caaaagccac      540

cuggaccaau uuuacaacau uugucuugau uucgacaacg auaagcugau cgaaauccuc      600

gugaaucaga accugaauau uuccgagaaa agcaagaaaa ucaaggaacu gcuccgcaag      660

acaaaccugg agguggggau ugccaaucuu aucgcuggag gcucuauuaa acuguccaac      720

auguuugacg augacgaacu caagaaucug gauuauaaca gccuuucuuu caaaaauucc      780

aacuucgacg agggcagaga gcuguuugaa gagguucucg aagaucgguu cgagcuggug      840

gaaauccuga aggacaucca cgaugccagc guccucgaga ccaugcuguc uuccgcuagc      900

auuuccccug acgggggaaa auacaucucu agcuccauga uuaauagcua uaacaagcau      960

aagcgcgacc uuaaacugcu caagaaaauc auuaagaaau acaagaaguc ugaguaugau     1020

aauaucuuua gagacgaaaa caucaaaaau aacuaccugu ccuauacacg gaccggcauu     1080

aacaagaaua aaaaggugca ggccgaggau aacacagaca gcgauggguu cuacaaauac     1140

aucguuaaga uucuuaagga caucaaagaa aaugauucug acgaggauau uaaguauauc     1200

cugagcgaaa uugacaaaaa gaacuuugug ccgaaacaga gaaccaagga aaauuccguc     1260

auccccuacc aacuccgcga gaucgaacug cggaaaauua ucgauaacca gucuaaguau     1320

cacaaguucc ugaaugaggu ggacgaaaaa ggaauuagcg ugaaggagaa aaucauuucc     1380

cuccugacgu uuagaauccc uuacuauauc ggcccccuua acccuagcua caaugaggaa     1440

aagaacuuca ccacaucuug ggucaaacgc cuggcgaagg ggaagguuac ccccuggaau     1500

uuugaggaua ugguggacau ugacgccucc cgggaggcuu ucaucgagaa caaacucaau     1560

aagugcagcu uucugcugga ugaaacagug cuuccuaaag acucucuccu guaucaggag     1620

uacauggucc ugaacgagcu caauaaccug aagauuaacg gacaguccau caauaccgau     1680

cuuaaaaaga gcauuuacga agaacuguuc aagaaauaua agaaagugac gagaaagcgc     1740

cucaucuccu acaucaaaga aaaggagaac augucuggcg acauuguuau cacaggcauu     1800

gauggggacu uuaagcaagg acugaaaagc uauaucgccu ucaaggauau uaucggcgaa     1860

aagaucgacg augacauuua ccggaaggag guggagaaaa ucauuagaga uaucucccug     1920

uauggggacg auaagucuuu cauucagaaa aagaucaaga augaaaacaa acuugaccuc     1980

agcgacgauc agauuuccaa gaucgcuaaa cugaaguuua aagacugggg acgcuucagc     2040

aagaagcugc ucgucggcau caaagccucu gauaagaaua ccggggagau ugacaacauc     2100

auuggauuua ugcggaauua caacuauaau aucaugcaac ugauggcgaa cguguucaca     2160

uuuaaagaug aaauugagaa acuuaauucc aaauacuaca ccgacgaaaa gaucaacauc     2220

gauuaucugg acgaucucua ccugagcucu ccggccaaga gaaugcugug gcaguccuuc     2280

cagauuguug acgagaucaa aaauauuaag aacagcgauc ccaaaaagau cuuuauugag     2340

auggcucgcu cuaaagaaga caagccuacc cggaagauga gcagaaaauc ccggcuugag     2400

aagcucuaua cagacagcaa aaagcaguuc aucaaacugu uuggccccga ugaauacgac     2460

aagcugaaua acgagaucaa gaacaaaucu gaguccgauu uccgcuggga caaucucuau     2520

cuguacuaua cgcaauuugg cagauguaug uacagccuug auccuauuga ccuggaggag     2580

cucuccaaca agaaucugua cgaucaggac cauaucuauc ccaaaucuaa gauuuacgau     2640

gacagcaucg aaaaccgggu gcuggucaaa aagcagcuua aucucgauaa auccaacgag     2700

uauccgauuc cugacagagu gcugaauaag gauaagaaca aacugcucgc cuucuggaag     2760

augcugaaug aaaaagagcu uaucggggac aagaaauacg cucgccugau ucggaagucu     2820

cccuuuaccg acgaggaacu cggagauuuc aucgccagac agcuggucga gacacgccaa     2880

gccaccaagg aaacagcuaa caugcugaaa agacuuugcc cugacagcaa gaucguguau     2940

guuaaagcga agaauguguc cgauuuccgg cgcaaauuug acauucugaa guguagaucu     3000

aucaacgauc uccaccacgc ccaugacgcu uaccugaaua uugucguggg caacguuuuc     3060

gauaccaagu uuacgagcaa uccccucaac uucaucaaaa auaacuccaa gaaccggagc     3120

uauaaucugg agaaaauuua cgacuacgau gugauccgca acgaaaagau cgccuggcuu     3180

gccgagagaa aagaggaaaa ucaggacggg ucuauugcua caguccugaa gaaccucucc     3240

aagaaugaua uccgggugac ccgcagaacc uauauuaaau gcgaggaccu guuugaugaa     3300

aacaucgugc ggaagggaaa uggcaaauuc cccauuaagg agaacaauaa caaaagcgac     3360

acaaauaagu acgggggcua uucuggaauc aaaaacgccu acuuuguucu gcuugaagac     3420

gagaagcaca aucgcauccu ggaggcgauu ccuuccuuca ucuauaagaa agaaaagaac     3480

ggcgagaaag ggagcgucau uaauuaccuc cagaacaucc ugggaauuaa gucugcuaaa     3540

auccucagag auaagauuaa gaucggcucc cugcuuaaaa ucgacaaguu uccguauaac     3600

cuggccggga aaagcggaaa uggcauucuc aucuccgggg gcgugcagcu gauucuggau     3660

aagaacacca gcaaauacau caagaagauu gaaaauuaca aauauaaacu ugacgagaac     3720

aaggauuacc aaaucaauau cuucgacaag cugucuaaag aggaaaacau uaagcucuuu     3780

gaucuguucg uggacaaguu cgagaauagc aucuauaaaa accgggucaa uauuaagcuc     3840

aaagaaauca agcuggccag acccaaauuu gagcaucuuu ccauugagga acagaucaag     3900

acacugacgc agaugcugau ccucuucgga cgcgagaaca augcugugaa ccuggaauac     3960

auuggccuuc cuaaaaauac cgggaagaca aagaucaaca aaaagauuaa uaacaaagau     4020

aucaagauua ucaaccaguc uauuaccgga cuguaugaga cagaagagga ccucgccaau     4080

aucuga                                                                4086


<210>  4224
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4224
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccaucggc       60

uucgccgugg ucgaugcuaa cucucaccuu cuuagacuga aaggggagac agccauugga      120

gcgcggcucu uucgcgaagg ccaaagcgcu gccgagagac gggaaucccg cacgaccaga      180

cggcgccugu cuagaacacg guggcgccuu agcuuccuga gagacuuuuu cgccccucau      240

aucaccaaga uugauccgga cuuuuuccuc cggcagaaau auuccgagau cucucccaag      300

gauaaagacc gcuuuaagua cgaaaagaga cuguucaaug aucggacaga cgcugaguuu      360

uaugaggauu acccuagcau guaucaccuu cgccuccauc ugaugaccca cacacauaaa      420

gccgacccca gagaaaucuu ccuugcuauu caccacaucc ugaagucccg ggggcauuuu      480

cucaccccug gagccgcgaa agauuucaac acggacaagg uugaucugga ggacauuuuu      540

cccgcccuua ccgaggcuua cgcccaggug uauccugacc uggaacucac auucgaucug      600

gcuaaagccg acgauuucaa ggcgaagcug cucgacgagc aggccacccc gagcgauaca      660

caaaaagcuc uggugaaucu ucugcucucu uccgacggcg aaaaggagau cgucaaaaag      720

agaaaacagg ugcugaccga auuugccaag gcuauuacgg gccuuaaaac aaaguucaac      780

cuggcccucg ggaccgaggu ugaugaagcc gacgcuagca auuggcaguu uucuauggga      840

cagcuggaug acaagugguc caacaucgag acaagcauga ccgaucaagg caccgagauu      900

uucgaacaga uccaggagcu guaccgcgcg cggcuccuga augggaucgu gcccgccgga      960

augucucuuu cccaggcuaa agucgccgac uacggccaac acaaggaaga ccuggagcuc     1020

uuuaaaacau aucugaagaa acuuaacgau caugaacugg ccaagacgau uagagggcuc     1080

uacgaccgcu auaucaaugg agaugacgcu aagccuuucc ugcgggagga uuuugugaaa     1140

gcccugacca aggagguuac agcgcacccc aacgaaguga gcgagcagcu ccugaauaga     1200

augggccagg cuaacuucau gcuuaaacaa cgcaccaagg ccaauggcgc cauuccuguc     1260

cagcugcagc agcgggaacu cgaccaaauc auugcuaacc aguccaaaua cuaugauugg     1320

cuggccaagc ccaacccggu ggagaagcac agagcuucua aaccuuacca gcuggacgaa     1380

cuucucacau uucgcauccc cuauuacgug gggccucuga ucacccccaa ggagcaggcc     1440

gagagccggg aaaaugucuu cgcguggaug auuagaaaag aucccuccgg aaacaucacg     1500

ccuuacaauu uugacgagaa gguugauugc ccggccagcg cuaacauguu cauucaacgc     1560

augaaaaaua cagacaccua ucugaucggc gaagaugugc uccccaagcg gucucugcuu     1620

uaccagaagu augaggugcu gaacgagcuc aauaacgucu ccauugacgg gagacggcug     1680

ggagccgacc agaaacagcg ccugaucaga gaaguguuug agcggcauag cucuguuacc     1740

aucaagcaag uggcugaaaa ucuugucgcc cacggcgauu ucgcgaaaag accugagauu     1800

cgcgggcucg ccgacgaaaa gcgguuccug agcucccugu cuacauacca ucagcucaaa     1860

aagauccugc acgaggcuau ugaugacccc accaaagagc augauaucga aaacauuauc     1920

acauggagca cgguguuuga ggacgccgcu auuuucaaga ccgaacuugc caagaucucc     1980

uggcuggaua gcgacacaau caaagcccuc ucuaauauua gauaucgcgg auggggccag     2040

uuuuccagaa agcugcugga uggccuuaaa cucgggaacg gacacaccgu uauccaggag     2100

cugaugcuga gcaaucacaa ccucaugcaa auucugacag acgaaacccu uaagacgacc     2160

auggcugagc ugaaucagga uaaacucaag gcggacgaua ucgaggacgu gauuaacgac     2220

gccuacacau cuccuuccaa caagaaagcu cugcggcagg uccugcgcgu ggucgaagau     2280

aucaagcaug ccgccaaugg ccaagacccc agcuggcuuu auaucgagac cgcugauggg     2340

ccgggaacag ccggcaaaag aacccagucc cggcagaagc agauucaaac gguguacgcg     2400

aacgcugccc aggaacucau cgacucugcc guucgcgggg agcuggaaga uaaaauugcu     2460

gacaaggcca gcuucacaga uagacuggug cucuacuuua ugcagggcgg acgggacauc     2520

uauaccggcg cgccucugaa uauugaucag cuuucccacu acgacaucga ucauauccug     2580

ccccaaucuc ucauuaagga cgacagccug gauaaccgcg uccuggugaa ugcuacaauc     2640

aacagagaga aaaauaacgu uuucgccucc acccuuuuug ccagcaagau ggcugccacc     2700

uggcggaaau ggcacgaggc ugggcugauu ucuggacgca agcucagaaa ucugaugcuc     2760

cggccugacg aaaucgauaa auucgcccac ggcuuugugg cgagacagcu ggucgagaca     2820

cgccagauua ucaagcugac ggaacagauc gccgcugccc aauaucccaa caccaagauu     2880

aucgcuguga aagccgggcu gucccaucag cuccgggagg agcuggacuu cccuaagaau     2940

agagauguga accacuacca ucacgccuuu gacgcuuucc uggcggcccg cauuggaaca     3000

uaucuucuga aacgguaccc caagcucgcu ccguuuuuca ccuauggcga auuugccaaa     3060

guugauguca agaaauucag agaguucaau uuuaucgggg cccugacaca ugcuaagaag     3120

aacauugugg ccaaagacac cggcgaaauc gugugggaua aggagcgcga cauucaggaa     3180

cucgaucgga ucuacaauuu caaaagaaug cugaucacgc acgaggucua cuuugagaca     3240

gcggaccuuu ucaagcaaac cauuuaugcu gccaaagaua gcaaggaacg cggaggcucu     3300

aagcagcuga ucccuaaaaa gcagggguac cccacccagg uguauggagg cuacacacgg     3360

gagaacaccg ccuaucucgc uguugugaaa acagucgaaa agaaagccga cguguacaga     3420

guugugcgga uugcgacguc ccaagucgcu gcccugaagg aggcccgcgc ucagagcacc     3480

gugaaggaaa gagagcugcu uaaaaaguuu cugacaacca aauucacaaa gaucgggaaa     3540

aacggaaaga aggccauuac ccccuuugac aucguuaucc cucgggugcc gcgcgagcaa     3600

cucuuccaca auaaaagaua uggcuuuuuc auggucaacu cugauacgcu gaugcauaau     3660

uaccaggaac ucuggaugag ccgguccgac cagcagauuc ugagcaagcu ggugaaaucu     3720

gcuaagaccg uggagaacgg gcaggucggc agacuguucg aaaauaucgu ugagcagauu     3780

aacaaauacu uuccccugua ugauaucaau caauuccgca agaagcucga ccaguccaaa     3840

gauauuuuua gcaagcugcc ucuugaagga aacggcacaa ccuccgggaa acgggagaca     3900

auccagaaua uccugaccgg agccggcaca ggggcgaaga acggagaccu caaaaaucug     3960

ggcauuaaga cccccuucgg guuucuccag gugccuucug gcaucgugcu ggaugacgau     4020

acgacacuua uuuaccaaag ccccaccgga augcugaaca gaaucguccg ccuguccgag     4080

cucuga                                                                4086


<210>  4225
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4225
augaagaacg aggaaaauau cuaccugggc cucgacauug ggaccgauag cgugggauau       60

gccgucacag acgauaaaua caagaucaug aaauaucacg gcaacgcugc cugggggucc      120

acgauuuucg acgcgggagc ucugggcgcc gauagacggg uuuuucgcuc ugccagacgg      180

cgccuugaca gacggcagga gcgcauccaa cugcuccagg aaauuuucgc uaaugagauc      240

gccaaggugg augaaagauu uuucauccgg cugcuugaga gcgggcugug gcgcgcugac      300

gccgaggaua gauacguguu uuucaacgac aaagaauaua ccgaugucca guacaugcgg      360

gacuauccca caauucauca ccucaucugc gagcugauuu acaauccuaa caagcaugau      420

gugcgccugg uuuaccucgc gugugccugg cuggugaccc acagaggaca uuuucuuucc      480

aauaucaaca cagaaaauau ugcugaccug gucgacaucc acaacgugua uaauggcuuc      540

augcgguuuu ucgaggagaa cgaaauuauc ugcgauuggc gcgagguuaa uguggaggaa      600

cucggcagag uccuggccgc uaacaucggg auuaaugaaa aguaccggaa ccuuucuacc      660

gugcuguuug ccacaagaaa accggacaag aaagcgaagg agggauuccc cuauagcgag      720

gaugccaucg ugcgccuccu ggcuggcggg accuguaaac ugaaggacgu cuacauuaag      780

gaagaguaug augaauuugg auccaucacg cucggcaugg acgaugagaa aaugaccgaa      840

auuauggccg acaucgggga ugacuucgau cugaucaagg cucugcgguc ucuguacgac      900

ugggggauuc ucgccaacau ccugguuaau gagaacaaua gcccucuuau uucccagagc      960

aaagugaagg uguaugagca acacagagaa gaccuggcca accucaaaua ccugguccau     1020

aaguacaucc cccacaaaua uaaugagauu uuccgcgaaa uccggggcga uaacuacgug     1080

gcuuauucuu accauuauga gcugaagaau gacgaaaaga ugggcaaaaa ggcggauguu     1140

gagaaauuuu ccaaguuccu ccugaaaauc cuugagggga uuagcgugga cgaacaggau     1200

aaggucgugu acgacaacau gaucgagaga cugacacucc gccuguuucu uccuaagcag     1260

aaaaauaccg auaaccgggu uauuccccac cagcuguaug aauacgagcu caagucuauc     1320

cugaaaaaua caaagaacua ccugccuuuc cucaaagaga aggacuccga uggaauuagc     1380

aaugaagaca agaucauuuc uaucuuuaaa uucaagaucc cguauuuugu gggcccccug     1440

aacugcaauu cccaauucgc cuggauucag agaaaagcug augggaagau cuuuccuugg     1500

aacuucgagg acaaagucga uuuugacgcc agcgaacagg ccuucauuca gcgcaugacc     1560

aaccaaugua cguaccuucc cgaggaaucc gugcugccua agcagucucu cuauuaccag     1620

aaguuuaugg uccugaauga gaucaacaau auuaaaaucg acggacggaa gaucagcgug     1680

gcuaugaaac aagagcugua uaacgaaauu uucgagaaga aaaagagagu ucgcaaaaag     1740

gaugugaucg acuucuuugu cuccaauggc uaccuugaag ggggaaagga ggcccucauu     1800

agcggccugg augaacagau ccacacaaac cugucuuccu uccaugcguu ucggagacuc     1860

cugcagaauc acauucuuag cguggaggac guugagucua ucaucgaacg ggcuagcuau     1920

uccgaugaca aacgcagagu gcuucucuuc cugaagaaca aauacucuuu ucugaccgau     1980

gaggacauua aguacaucug caaaauuaag aucaaggauu ucggccggcu uagcaaaaag     2040

uuucucgucg acuucgaagg gacauguaaa gagaccggag aaauuuccac cgugcugaga     2100

cugauguggg agacaaauaa caaucucaug gagcugcuua gcgauaagua uacguuugcc     2160

aacggcauca aagaaaaucg caagaaguac uauaccgagc agcaaaagac acuggacgau     2220

cggcucgacg aaauguacau cucuaacucc gugaagagaa ccauuuaucg cacacugagc     2280

guugucaaag acgugaccaa ggccuucggg gugcccaaaa agaucuuugu cgagauggcu     2340

agaacgaaua acccggaucu gaagggaaaa cggacacgca ccagacagca acaggugauu     2400

gagcuuuacg ccaagugcga cgaagaggau guucgggaac ucaaacauca aauggagucu     2460

cugggcgaau auguggacaa caagcugcag uccgauaaac ucuuccugua cuuuauccag     2520

uucggaaaga gcgcuuacuc cggcaaaaug auugaccuug agaagcugau gucuggaagc     2580

aaggucuaug auaucgagca cauuuacccu caggccuaug ugaaagacga uuccaucauc     2640

aauaacaagg uucucguguu cucugaagag aauggcgcga aacaagacau uuaccccauc     2700

aagaccgaaa uucgcaaaca gaugagcggg aucuggacaa ccuggcauca cgucggagcc     2760

auuuccgaug agaaguauaa gagacugaca cggagcacgc cuuuuaacga agacgagaaa     2820

uacggcuuca ucaaucgcca gcugaccgag acaucucagu ccaccaaggc uaucgcccaa     2880

auucuuaacg aaaaauaucc cgagacagaa aucguguacg uuaaggcugg gcucgugagc     2940

gacuuuagac acgaguucgg acuguguaaa ugccggucuu acaaugaucu gcaucacgcc     3000

auugacgcgu aucucaacgu cguggugggc aaugucuaca acaugaaguu uaaucgcaag     3060

ugguucuccg uugauagcaa auauucuauc aagaccaaaa cgauuuuuag ccauucccug     3120

guguguaacg ggaagaucau cugggacaaa gaaaagaugc uucccauggu gcugaagaau     3180

gccaaaaaga acaaugcuca cuucaccaaa uaccaguuua ugaagacagg cggauucuuu     3240

gaucagaugc cuugcaaaaa ggccagcggc cucgucccga gaaagaaagg gcuggacacc     3300

gagcgguaug gaggcuacaa caagucuggg auuauguucc ugacacccau gagauauaaa     3360

aucggaaaga aauccgauau uaucauucuu agcaucgagc ugcucuacgg caagcuguuc     3420

cucaaagacg aaguguuugc uaagaaguac gccgaggaac gccugcagaa aauuaucggg     3480

aagaaagccg augagguuuc cuucccuaug ggcaugaagc ccuggaaaau caacaccgug     3540

cuuucucugg acggauuuaa ugucugcauu acgggcaucg ggggaggcgg gaagugucuc     3600

agcgcucagc cuauuaugca gcugucccug cccuaugagc uugcgaagua ccugaaaaag     3660

aucgaaaaau ucguggagaa gaacaaaaau aacgccaauu auauuuacga ugaacaguau     3720

gacucuguua gcguggauga gaacaucaag cucuacgacg ucuaucugga caagcucgaa     3780

aauuccaucu acaacaaacg gauuaauucu ccggaggcua uccugaagaa cggaagagag     3840

aaauuuugca aacuuaaugu gcuggaacaa gccguuacac ucauuaacau ccagagcauu     3900

uucggccgca ugaccggggg cugugaucug gagaauaucg gaggcaaaaa gcacuccgcc     3960

gcuacaguga acuuuagcuc uaaugucucc aacuggaaga aauacuauaa ugacgugcgg     4020

guggucuacc agagcccuuc ugggaucugg gaaagacgcu cccagaacau ucuggagcuu     4080

cuguga                                                                4086


<210>  4226
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4226
augaacgagc ccuacggcgu ggggcuggac aucggaacca auagcgucgg cuggacaguu       60

guggauauga acgggagagu gcggaagguc aaaggaaaga cggcccucgg cgcucgccug      120

uucaaagaag gggccaccgc ggaggacaga cggggauuuc gcacaaccag acggcgccuu      180

aagagaguga aauggcggcu gcgccuccug agagaguucu uugaucagcc uauuuccaag      240

aucgacccga auuucuuugc ucggcgcaag uauucugaua uuagccccag agacccuaac      300

uacaauggcc uugccaaaac acuguucaac gaucggaccg acaaggaauu uuaugaugac      360

uacgccacaa ucuaucaccu ccgcgauaaa cugaugaccu ccaauagaaa guucgacauu      420

cgggaaaucu accuggcuau ccaucacauu guuaaauaua gaggcaacuu ucuccgcacg      480

gggcccgccu cucaauacgg aagcgcuucc cugcaucuug ccaccucuuu ccagaagcug      540

aaugaucucu uugcgcagag cgaggagaca cugaaccuua agcuggugac cgacgaagcc      600

cuccugcagc aaauccagca gauucugguc cggacagacc ucuccagaag cgagcagcaa      660

cgccagaucu ggccucugau ggcugugcuu accggcgccu cugcugccga aaagaagcgg      720

cagaaaaaug uuguggucga gcuguccaag gcgcucgugg ggcugaaagc caagaugaac      780

guggucacgc uuacagaggu ugaugcugcc guggugaaag acuggaccuu cacaauggaa      840

gagagccagg auaagcugcc cgaaauugag gaacaacucu cugaggucgg acagcagauc      900

auggacgagg ugauucaacu guacgcuucc guuaaucugg cccagcucau cccugccggc      960

aagagauuua gccagcacau gguggaaaaa uauaagcauc acgagaaaaa ccuggaacuu     1020

cugaaggcuu acauccacuc ucaguccgau agcaaacgcg ggcgggagau uagagcgacc     1080

uaugaccgcu acaucgaugg agucgacucc aagccgguga cccaagaaau guucuauaag     1140

gaucucauga aauacguuga ggccgacgcu acaucuaauc aucuggccgc cgagauuaag     1200

gaugaaaucg acagcgagca guucaugccc aaacuucgga cgaagcagaa cggcuccauu     1260

ccuuaucagg ugcaacagua cgaacuggac cagaucauca gccaccagaa aaaguacuau     1320

cccuggcucg gcgaggaaaa cccugucgcu gagagacgcg ggaaguuucc cuacaaacug     1380

gaugagcugg ugggauuccg ggucccguau uacgugggcc cucucauuac caaggaagac     1440

caacaggcca caucuggggc gggauuugcu uggaugguua gaaaagccga uggccccauc     1500

accccuugga auuucgacca gaagguggau cggauugccu ccgcuacagc cuuuauccag     1560

cgcaugcaaa ccacggacac auaucugauu ggggaggaug uccuucccgc uagaagccug     1620

aucuaccagc gguucauggu gcucaacgaa cugaauaaca ugagaguuga ggaccgcaaa     1680

cuggcgcccc agcaaaagca gcggcuuuau aaucaggugu uuaagcagca ucaacacguc     1740

ucugugaaaa acauccagca gaaucucaug gaugccggag aguacagaaa gaccccucag     1800

auuaccggcc uggccgaccc gaaaggcuuc aacagcuccc ugucuacaua ccacgauuuu     1860

aagaaaaucc uccuggaagc uauugccgac gagcauaagc gcgcugauau cgagaaaauu     1920

auccuuugga gcaccacauu cgaagacucc gccauuuuua agcaaaagcu ggaagaggug     1980

gcguggcuca cggacgccca gagaaaacag cugagcgggc ugcgguaucg cggauggggc     2040

cagcuuucuc acaagcuccu gaccgcuuuc aaagaugaca aggggagauc caucauggau     2100

ggacuguggg agacaagcga caauuuuaug caacuccgga aacagcccgc caucgaaacc     2160

cagauuaagg aggcuaacca agccaaucug acagaugccg acauccagga uaccauuaac     2220

gaacuuuaca cgucuccuca gaauaagaaa gcuauccgcg agguuaugcu gguccucgac     2280

gauauuaaga acgcgaugca uggccagacc cccuccugga ucuucgugga agccgcuaga     2340

gggggcggag uggccggccg gcgcacacaa agcagauccu cucagaucgu cgaggccuau     2400

aaagggaccg cuaaggagau ugugagcgaa aaaguucagc acgagcugaa ugaaaagauc     2460

aaggccaaag cggacuucaa cacacggcug gugcuuuacu uucuccagaa cggacgcgau     2520

cuguauacca augaggcuau uaacaucgac agacuguccg aauacgauau ugaccauauc     2580

cucccucaau cucuggucaa ggacgauagc cuugacaauc gggugcugac guccgccaga     2640

aucaaccgcg agaaaaauga uacauucgcc agcgagaagu uuggccggaa aaugggggcu     2700

caguggagag aacuccaccg caacggacug augacccagc ggaagcugaa gcaccuucuc     2760

augagacccg acgagauuuc uaaacaugcc acagauuuca ucaaucgcca gcugguugaa     2820

acccggcaag ugauuaagcu ggucgaggag cucaucucca gcgaauaucc ggcggcuucu     2880

auuguggccg uuaaagccaa ccugacccac caguuuagac agacauucaa uuuuccuaaa     2940

cuucgcgagg ugaacgacua ccaucacgcu uucgaugccu cccugacggc uuuuaucggc     3000

auguaccucc ugaaacagua ucccaagcug gaacgguucu uugucuacgg gaaauucgcc     3060

aagcaaccua ucaaucuuac cagauuuaac auuauccgga agcuggcggu ggccgagaaa     3120

cccauugcua auaucgaaac aggcgagauu cucugggaca agaccgccga uaucaaauau     3180

uucgagaagc uguacaacua uaaacgccuc cuggugacac acgaagucag agagaauuac     3240

ggagcuaugu ucaagcagac ccuuuuuaag gccagcuaua acaaaucuaa gacgcugguu     3300

ccuaaaaaga accauaugga aacaagcgug uacggcgggu acuccaauca ggagaccgcc     3360

uaucucgcua uugugcggau ccccuucaaa agcggauuua aguucaucgu cgugggcauu     3420

ccgacccgca ugguugcgaa gaucaaacac uaccaaucuc ugggggccac acugaaccag     3480

gcuacccaua aggugauuga accuaaauuu acaaagaucu ccagaaaaac gaagcagacc     3540

gucauuagcg acuaugaggu gguucuuccc aagguguacc uggaucaggu cgugcgggac     3600

caaguuaaag gacagaugua uagauucucc cucggcucug acaaggagua ccacaaugug     3660

caggaacugu aucucccccu gagcauccag caagccuuug ucgggcauua cgaugagucc     3720

gacgaucagc gcucuaacga ccuugugaaa guguacgaug ccgcucugaa gcagcuccag     3780

cgguauuucc cucugcaccu gagcagaaau uuugaccaag ucgccuccca ggcgcaccag     3840

ucuuucgaag gccuuaaaaa caauguucaa acaagcgaua agcagcuggg aaagaaagag     3900

gugcucaacu cccuguuugu gggccuccau gcuaaugcca cccgcagcaa ccugucuguc     3960

cuugggaugu ccaaggacuu cggacggauc aaaagcaaug gcauuacacu gaccgaucag     4020

gccgaaauca uuuaccaguc uccgacgggg cuguucgaga gaaagguggc ucucaaagac     4080

cuguga                                                                4086


<210>  4227
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4227
augggcaacu acuaucuggg gcucgacauc ggaaccaaua gcgugggcug ggccgucaca       60

gaugagaacu acaauauuga aagauucaag aaaaagaaca ugugggggau ccggcuguuu      120

gacgaggcua aaacggccga aucccgccgg auucaccggc aggcgcgcag aaugcggcaa      180

cgcaagaucc agagacuuaa ucugcucaug gagcuguucg auguugaaau uucuaaagug      240

gacgagagcu uuuucauccg gaagaaggag uccgaucuuc auuaugaaga caaaucucag      300

gauagccgcu acgcucuguu uaacgacaag gaguucaaug auaaagacua ucacaacaag      360

uaccccacca ucuuucaucu cauuauggau cugaucgaaa augacgagaa aaaggauauu      420

agacugcucu aucuggccug ccacuaccuu aucaagaacc ggggacauuu cauuuuugag      480

ggccagaaau ucgacgucaa uuccucucug aacaauaaca ucaauaagcu caaaagccac      540

cuggaccaau uuuacaacau uugucuugau uucgacaacg auaagcugau cgaaauccuc      600

gugaaucaga accugaauau uuccgagaaa agcaagaaaa ucaaggaacu gcuccgcaag      660

acaaaccugg agguggggau ugccaaucuu aucgcuggag gcucuauuaa acuguccaac      720

auguuugacg augacgaacu caagaauuuc gauuauaaca gccugucuuu caaaaauucc      780

aacuuugacg agggcagaga gcuuuucgaa gagguucugg aagaucgguu ugagcucgug      840

gaaauccuga aggacaucca cgaugccagc guccuggaga ccaugcucuc uuccgcuagc      900

auuuccccug acgggggaaa auacaucucu agcuccauga uuaauagcua uaacaagcau      960

aagcgcgacc ugaaacuucu gaagaaaauc auuaagaaau acaagaaguc ugaguaugau     1020

aauaucuuca gagacgaaaa caucaaaaau aacuaccucu ccuauacacg gaccggcauu     1080

aacaagaaua aaaaggugca ggccaaagau aacacagaca gcgauggguu uuacaaguac     1140

aucguuaaga uucugaaaga caucaaggag aaugauucug acgaagauau uaaauauauc     1200

cuuagcgaga uugacaagaa aaacuucgug ccgaagcaga gaaccaaaga aaauuccguc     1260

auccccuacc aacugcgcga gaucgagcuc cggaagauua ucgauaacca gucuaaguau     1320

cacaaauuuc ugaaugaagu ggacgagaag ggaauuagcg ugaaagaaaa gaucauugcg     1380

cugcucacgu ucagaauccc uuacuauauc ggcccccuga acccuuccua caaugaggag     1440

aaaaacuuua ccacaagcug ggucaagcgc cuugccaagg ggaaaguuac ccccuggaau     1500

uucgaagaua ugguggacau ugacgcuucu cgggaggccu uuaucgaaaa caagcugaau     1560

aaaugcuccu uccuccugga ugagacagug cugccuaagg acagccuucu cuaucaggaa     1620

uacauggucc ugaacgagcu gaauaaccuc aaaauuaacg gacagucuau caauaccgau     1680

cugaagaagu ccauuuacga ggaacuuuuu aaaaaguaua aaaaggugac gagaaaacgc     1740

cugaucagcu acaucaagga gaaggaaaac acauccggcg acauuguuau caccggcauu     1800

gauggggacu ucaaacaagg acucaagucu uauaucgcuu ucaaagagau uaucggcgaa     1860

aagaucgaua augacauuua ccggaaagag guggagaaga ucauuagaga uaucagccug     1920

uauggggacg auaaauccuu uauucagaag aagaucaaaa acgaaaauaa gcuggaccuu     1980

ucugauaacc agauuagcaa aaucgccaag cucaaauuca aggacugggg acgcuuuucc     2040

aagaaacugc uggucggcau caaggcgagc gacaaaaaua caggggagau ugauaacauc     2100

auuggauuca ugcggaauua caacuauaau aucaugcaac ucauggccaa cguguuuacc     2160

uucaaggacg aaauugagaa acuuaauucu aaguacuaca ccuaugaaaa gaucaacguu     2220

gauuaccuug acgaucugua ucucuccgcu ccggccaaaa gaaugcugug gcagagcuuu     2280

cagaucgugg acgagauuaa gaauaucaaa aacucugcuc ccaagaaaau uuucaucgag     2340

auggcccgcu ccaaggaaga uaagccuaca cggaaaauga gcagaaaguc ucggcuggag     2400

aaacuuuaca cggacagcaa gaaacaguuu auuaaacucu ucggccccga ugaauaugac     2460

aagcugaaca augagaucaa gaacaagucc gagagcgauu uucgcuggga caaucuguac     2520

cucuauuaca cccaauucgg cagauguaug uacucucugg acccuaucga ucuugaagaa     2580

cuguccaaca aaaaucucua ugaccaggau cauauuuacc ccaagagcaa aaucuaugac     2640

gauuccauug aaaaccgggu ccuggugaag aaacagcuga aucuugacaa gucuaacgag     2700

uacccgaucc cugauagagu ccucaauaag gacaaaaaca agcugcuggc cuuuuggaaa     2760

augcucaaug aaaaggagcu gauuggggau aaaaaguaug cucgccuuau cagaaagagc     2820

cccuucacag acgaggaacu gggagauuuc auugcgagac agcucgugga gacccgccaa     2880

gccacaaaag aaaccgcuaa caugcugaga cggcugugcc cugacuccaa gaucguuuac     2940

gugaaagcca agaaugucuc ugauuuucgc agaaaauucg acauccuuaa gugucggagc     3000

auuaacgacc ugcaccacgc ccaugaugcu uaucucaaua ucgugguugg caacguguuu     3060

gacacgaagu ucacauccaa uccccugaac uuuauuaaaa acaauucuaa gaaccgcagc     3120

uacaaucucg agaaaaucua cgauuaugac gucauuagaa acgaaaagau cgccuggcug     3180

gcggagcgga aagaggaaaa ucaggauggg uccaucgcua ccgugcuuaa gaaccugagc     3240

aagaaugaca uucgcgugac cagacggaca uacaucaaau gcgaggaucu cuucgacgaa     3300

aacauuguuc gcaagggaaa uggcaaauuu ccccugaagg agaacaauaa caaaucugau     3360

accaauaagu augggggcua cuccggaauc aaaaacgccu auuucguccu gcuugaagac     3420

gagaagcaca auagaauuau cgaggccauc ccuagcuuua uuuacaagaa agaaaagaac     3480

ggcgagaaag ggucugugau caacuaucug cagaauauuc ucggaaucaa guccgcuaaa     3540

auucugcggg auaagaucaa gauuggcagc cuccugaaaa ucgacaaguu cccguacaac     3600

cuugccggga aaucuggaaa uggcauccug auuuccgggg gcgugcagcu cauccuggau     3660

aagaacacaa gcaaauacau uaagaagauc gaaaauuaua aauacaagcu ggacgagaac     3720

aaagacuauc aaauuaauau cuuugauaag cuguccaaag aggaaaacau caagcuguuc     3780

gaccucuucg ucgauaaguu ugagaauagc auuuacaaaa acagagugaa uaucaagcug     3840

aaagaaauua agcucgcucg cucuaaauuc gagcaucuga gcaucgagga gcagauuaag     3900

acgcuuaccc agaugcugau ccuguuugga cgggagaaca augccguuaa ccucgaauau     3960

aucggccugc ccaaaaauac agggaagacc aagauuaaca aaaagaucaa caauaaagac     4020

auuaagauca uuaaccaguc caucacagga cuuuacgaga ccgaagagga ucuggcgaau     4080

auuuga                                                                4086


<210>  4228
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4228
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

aucacagagg auuauaaggu uccugcuaaa aagaugaaag ugcucggcaa uacgucuaga      120

gacuucauua agaagaaccu gcuuggggcc cugcucuuug auagcggaga aaccgcggag      180

gcucggcgcc ugaaaagaac agcccggcgc agauacaccc ggcgcagaaa ucggcuucgc      240

uaucugcagg aaaucuuccu cgacgagaug aacaagcugg auuccggcuu uuuccacaga      300

cuggacgauu cuuuucucgu gccggaagag aaacggggga gccgcuaccc cauuuucgga      360

acacugcaag aggaagucaa uuaucaugag aacuuuccua ccaucuacca ccuuagaaag      420

aaacuggccg acuccaagga aaaggcugau cuccggcuga ucuaucuugc ccuggcucau      480

augauuaaau accgcggcca cuuccucuac gaggagucuu uugacagcaa uaacaauucc      540

auccaggaaa uuuucaacga guuuacagcc gaauaucaua auaccaucga gggcagcugc      600

cugucugggc agauugaaca gguggaggcg auccugacgg auaagauuuc caaaagcgcc      660

aagagagagc ggauccucaa acuguucccc gcugaaaagu cuaccggacu uuucuccgag      720

uuccugaaac ucaucguugg caaccaagcc gacuucaaga agcacuuuga ucuggaagag      780

aaaacaccuc uucaguucag caaggacacc uacgaagagg accuggagaa ucuccuggaa      840

cagauugggg augaguuugc ugaccuguuc cuccuggcca aaaagcugua ugaugcgaca      900

cugcucgccg gaauccugac cgcuacggac gugucuacaa aagcuccccu uuccgccagc      960

augauuaaca gauacgaaac ccaccagcaa gaucuggccg cccucaagca guuuaucaag     1020

aauaaacugc cugagaagua uuccgaaguc uucucugacc ugagcaaaga uggcuacgcu     1080

ggguauauua acggaaagac aacccaggag gcguuuuaca aauacaucaa gaaccuccug     1140

aucaaguucg agggcgccgu ggacuuucuu gagaaaauug agcgcgaaga uuuccugcgg     1200

aagcagagaa ccuuugacaa uggcuccauc ccgcaucaac uccaccuuca ggagaugaac     1260

gcuauucugc gccggcaggg ggaucauuau cccuuccuua aagagaauag agaaaagauc     1320

gagaaaauuc ucacauuucg caucccuuac uauguuggac cccuggcccg gggcaacaga     1380

gacuucgccu ggcugacgcg caauagcgac caagcuaucc ggccuuggaa cuucgaagag     1440

guggucgaua agaccucuuc cgccgaagac uuuauuaaua agaugacaaa cuacgaucuc     1500

uaucugcccg aggagaaagu gcuuccgaag cacagcaucc uguacgaaac cuucgcggug     1560

uauaaugagc ucacaaaagu caaguuuauu gcugaagggc ugagagacua ccaguuccug     1620

gauucuggac agaaaaagca gaucauuaac cuucucuuua aagacaagcg caagguuacc     1680

gagaaagaua ucauucacua ccugcauacg guggacggcu augaugggau cgaacugaag     1740

ggaaucgaca aacaauucaa ugccagccuc uccacauacc acgaucugcu uaagauuauc     1800

aaagacaagg auuuuaugga cgacgccgau aacgaggcua uucuggagaa uaucgugcau     1860

acccucacca uuuucgaaga ccaggagaug aucaagcagu cucuggccca guaugaugcu     1920

aucuuugacc ggaaagucau gaaggcccug acaagacggc acuacaccgg cuggggcgaa     1980

cuuagcgcga aacucauuaa cgggauccgc gauaagcaaa caggaaaaau gauucuggag     2040

uaucugaagg acgauggcga aaucaauaga aacuucaugc agcucauuaa cgacgauucc     2100

cugagcuuua aggagacgau ccagaaagcc caggugaucg gggagaccga aaauauuaag     2160

caaaucguuc aggagcuucc uggaucuccc gcuauuaaaa agggcauccu gcaguccauu     2220

aaaaucgugg acgaacucgu cgagaucaug ggguacaacc cugagagcau ugugguugaa     2280

auggcccggg agaaucaguu cacaaaccaa ggcagacgca auucucagca gcggcugaag     2340

aagcugaccg auuccaucaa caaacuugga agcuccauuc ucgaagaaca ucccguggac     2400

aauucucaac ugcagaacga uagacuguuu cucuauuacc ugcagaaugg caaggacaug     2460

uacacagggg aagcucuuga gcugagcaaa cucuccucuu augacgucga ucacaucauu     2520

ccccaggccu ucaucaagga cgauagccug gacaaccgcg ugcuggucuc cuacgcgaaa     2580

aauagaggaa agagcgauga cgugccuucu cuugagauug uucaaaaacg gaaggccuuc     2640

uggcgccagc ugcucgauuc ccagcugauc agcgaaagaa aguuuaacaa ucucaccaaa     2700

gcugagcggg gcgggcugac ggaagacgau aaggccggau ucaucaaacg ccagcuugug     2760

gagaccagac aaauuacaaa gcacgucgcu cagauccugg acgcccgguu uaacaccgaa     2820

gugacagaga aaggcaagaa gaaacgcgcc guuaagauua ucacccucaa aucuaaucug     2880

guguccaacu ucagaaagga uuuugagcug uauaaagucc gggaaauuaa cgacuaccau     2940

cacgcucaug acgcguaucu uaaugccgug guggggacgg cucugcucaa gaaguacccg     3000

aaacuggagc ccgaauuugu uuauggcgau uacccuaagu acaacagcua ucgcgagaga     3060

aaaacagcca ccgaaaaggu cuacuuuuau ucuaauauca ugaacuucuu uaaaacaacc     3120

gugaagcucg ccgacggaag cgagaagaaa cggcaccaga ucgagaccaa ugaagagaca     3180

ggcgaaauug ugugggauaa gacgaaacac uucgagaucg ucagaaaggu gcuguccuac     3240

ccccagguua acauugugaa aaagaccgag gaacaaacag ucgggcagaa uggaggccuu     3300

uuugacaaca auaucgugag caagaacaaa guuguggaug cuucuaagcu gauuccuauc     3360

aaauccgggc ucagccccga gaaguaugga ggcuacgccc gcccgaccau cgcguauucc     3420

guccugguga uugcugacau cgaaaaaggg aaggccaaaa agcugaagcg gauuaaagag     3480

augguuggca ucacaauuca ggauaagaaa aaguucgaag ccaauccuau cgcuuaccuu     3540

gaggagugug gauucaaaaa cauuaauucu aaccauauca ucaagcugcc caaguacagc     3600

cucuuugagu ucgacgaugg ccaaagacgc cugcucgccu ccucuauuga acugcagaaa     3660

gggaaugaac uuauccugcc uuaucaccug accgcgcucc uguaccaugc ucagcggauu     3720

aacaagauca gcgagcccau ucacaaacag uauguggaag cccaucaaaa cgaguuugag     3780

gaacuucuga cgacaaucau cucccucucu aagaaauaca uucagaagcc uaaugucgag     3840

agccugcucc agcaggccuu cgaccaaacc gaugaagaca ucuaucagcu guccgagagc     3900

uuuauuucuc uucugaagcu gaucuccuuc ggagcucccg gcgccuuuaa auuccucggg     3960

guggauauua gccagucuaa cgugagauac caguccguca gcucuugcuu uaaugcuacc     4020

cugauccacc aauccaucac aggacuuuau gaaacccgca uugaccugag caagcucggc     4080

gaggacuga                                                             4089


<210>  4229
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4229
augaacgagc ccuacggcgu ggggcuggac aucggaacca auagcgucgg cuggacaguu       60

guggauauga acgggagagu gcggaagguc aaaggaaaga cggcccucgg cgcucgccug      120

uucaaagaag gggccaccgc ggaggacaga cggggauuuc gcacaaccag acggcgccuu      180

aagagaguga aauggcggcu gcgccuccug agagaguucu uugaucagcc uauuuccaag      240

aucgacccga auuucuuugc ucggcgcaag uauucugaua uuagccccag agacccuaac      300

uacaauggcc uugccaaaac acuguucaac gaucggaccg acaaggaauu uuaugaugac      360

uacgccacaa ucuaucaccu ccgcgauaaa cugaugaccu ccaauagaaa guucgacauu      420

cgggaaaucu accuggcuau ccaucacauu guuaaauaua gaggcaacuu ucuccgcacg      480

gggcccgccu cucaauacgg aagcgcuucc cugcaucuug ccaccucuuu ccagaagcug      540

aaugaucucu uugcgcagag cgaggagaca cugaaccuua agcuggugac cgacgaagcc      600

cuccugcagc aaauccagca gauucugguc cggacagacc ucuccagaag cgagcagcaa      660

cgccagaucu ggccucugau ggcugugcuu accggcgccu cugcugccga aaagaagcgg      720

cagaaaaaug uuguggucga gcuguccaag gcgcucgugg ggcugaaagc caagaugaac      780

guggucacgc uuacagaggu ugaugcugcc guggugaaag acuggaccuu cacaauggaa      840

gagagccagg auaagcugcc cgaaauugag gaacaacucu cugaggucgg acagcagauc      900

auggacgagg ugauucaacu guacgcuucc guuaaucugg cccagcucau cccugccggc      960

aagagauuua gccagcacau gguggaaaaa uauaagcauc acgagaaaaa ccuggaacuu     1020

cugaagaccu acauccacuc ucaguccgau agcaaacgcg ggcgggagau uagagcuacc     1080

uaugaccgcu acaucgaugg agucgacucc aagccgguga cacaagaaau guucuauaag     1140

gaucucauga aauacguuga ggcggacgcc acgucuaauc aucuggcugc cgagauuaag     1200

gaugaaaucg acagcgagca guucaugccc aaacuucgga ccaagcagaa cggcuccauu     1260

ccuuaucagg ugcaacagua cgaacuggac cagaucauca gccaccagaa aaaguacuau     1320

cccuggcucg gcgaggaaaa cccugucgcc gagagacgcg ggaaguuucc cuacaaacug     1380

gaugagcugg ugggauuccg ggucccguau uacgugggcc cucucauuac aaaggaagac     1440

caacaggcua ccucuggggc cggauuugcg uggaugguua gaaaagcuga uggccccauc     1500

acaccuugga auuucgacca gaagguggau cggauugccu ccgccaccgc uuuuauccag     1560

cgcaugcaaa cgacagacac cuaucugauu ggggaggaug uccuucccgc cagaagccug     1620

aucuaccagc gguucauggu gcucaacgaa cugaauaaca ugagaguuga ggaccgcaaa     1680

cuggcucccc agcaaaagca gcggcuuuau aaucaggugu uuaagcagca ucaacacguc     1740

ucugugaaaa acauccagca gaaucucaug gaugccggag aguacagaga agcgccuaag     1800

auuaccggcc uggccgaccc gaaaggcuuc aacagcuccc ugucuacaua ccacgauuuu     1860

aagaaaaucc uccccgaggc uauugccgac gaacauaagc gcgcugauau cgagaaaauu     1920

auccugugga gcaccacauu cgaagacucc gccauuuuua agcagaagcu ggaggaggug     1980

gcguggcuga cggacgccca aagaaaacag cucagcgggc ugcgguaucg cggauggggc     2040

cagcugucuc acaaacuucu caucgcuuuc aaagaugaca augggagauc caucauggau     2100

ggacuguggg aaaccagcga caacuuuaug cagcugcgga agcaaccugc cauugagaca     2160

cagaucaaag aagcuaauca ggccaaccuc accgaugccg acauucaaga uacaaucaau     2220

gagcuguaca ccucucccca gaacaagaag gcuauucgcg aaguuaugcu uguccuggac     2280

gauaucaaaa augcgaugca uggccagacg ccuuccugga ucuucgugga ggccgcuaga     2340

gggggcggag uggccggccg gcgcacccag agcagauccu cucaaauugu cgaggccuau     2400

aaggggacag cuaaagaaau cgugagcgag aagguucagc acgaacucaa cgagaaaauu     2460

aaggccaagg cggacuucaa cacccggcug gugcuguacu uucuucagaa uggacgcgau     2520

cucuauacaa acgaagcuau caauauugac agacuguccg aguacgauau cgaccauauc     2580

cugccccagu cucucgucaa agacgauagc cuggacaacc gggugcuuac cuccgccaga     2640

auuaaucgcg agaagaacga uacguucgcc agcgaaaaau uuggccggaa gaugggggcu     2700

caauggagag agcugcaccg caauggacuc augacacagc ggaaacugaa gcaccuucuu     2760

augagaccgg acgaaaucuc uaagcaugcc accgauuuca uuaaccgcca gcucguugag     2820

acaagacagg ugaucaaacu ggucgaggaa cugauuucca gcgaguaucc ugcggcuucu     2880

aucguggccg uuaaggccaa ucucacccac caauuucggc agaccuucaa cuuucccaaa     2940

cugcgcgaag ugaaugacua ccaucacgcu uucgaugccu cccuuacagc uuuuaucggc     3000

auguaccugc ucaagcagua uccuaaacug gagcgguucu uugucuacgg gaaguucgcc     3060

aaacagccca uuaaccugac gagauuuaau aucauucgga aacuugcggu ggccgaaaag     3120

ccuaucgcua acauugagac cggcgagauc cugugggaca aaacagccga uauuaaguau     3180

uucgaaaaac ucuacaauua uaagcgccug cucgugaccc acgaggucag agaaaacuac     3240

ggagcuaugu ucaaacaaac acuguuuaag gccagcgaca acaagucuaa aacccuuguu     3300

cccaagaaaa aucauaugga gacgagcgug uauggcgggu acuccaacca ggaaacagcc     3360

uaccuggcua ucgugcggau cccguucaag agcggauuua aauucauugu cgugggcauc     3420

ccuacccgca ugguugcgaa gauuaagcac uaucagucuc ucggggccac ccugaaacaa     3480

gcuacacaua aggugaucga gcccaaauuu accaagauuu ccagaaaaac aaagcagacg     3540

gucaucagcg auuacgaggu gguucugccc aagguguauc uugaccaggu cgugcgggac     3600

cagguuaaag gacaaaugua cagauucucc cugggcucug auaaggaaua ucacaaugug     3660

caggagcucu accugccucu cagcauccag caggccuuug ucgggcauua ugacgaaucc     3720

gaugaccaac gcucuaacga ucuggugaaa guguacgacg ccgcucuuaa gcagcugcag     3780

cgguacuucc cgcuccaccu gagcagaaau uuugaucagg ucgccaacca agcgcaccag     3840

uccuucgagg gccugaaaaa uaacguucag aagucugaca agcaacuugg aaagaaggaa     3900

gugcugaaua gccucuuugu gggccugcau gcuaccgcca cacgcuccaa ccucagcguc     3960

cuggggaugu cuaaagauuu cggacggcuu caguccagaa gcggcauuac ccugacagac     4020

caggccgaga ucauuuauca gucucccacc gggcuguucg agcgcaaggu ggcucucaaa     4080

gaucuguga                                                             4089


<210>  4230
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4230
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uuggcagcaa cuccaucggg       60

uucgccgcug ucgaugaaaa uucucaccuc auuagacuga agggaaaaac aguuaucggc      120

gcccggcuuu uugaagaggg gaaggcggcu gccaaacgca gagccggacg gacgacccgc      180

agacggcuga gccgcaacag auggcggcuc uccuuccugc gcgacuuuuu cgaaucucau      240

auuacaccua ccgauccgaa cuuuuucaug agacagaagu auagcgagau cucccccaag      300

gacaaagcuc gguacaagua ugaaaaacgc cuuuuuaaug auagaacaga cgccgaguuc      360

uaccaacagu auucuaccau guaccaccuu cggaaucgcc ucaugacaga uccuagcaga      420

gcugacgugc gggagaucua uuuugccauu caucacaucc ugaaguccag aggccauuuc      480

cugacccccg gcgaugcgaa agacuuuaac acgaaugaug uggcccucga cgaaauuuuc      540

ccugcucugc aggaugccua cgcucagguc uaccccgacc uugggaucac cuuugacgag      600

gauaaggcca acgaguucaa gacaauucug cucaaugaga ucgcgaccag cauugacaca      660

caacgcgccc uggugaaacu ucugcucgcu gaggaagaug acaaggauau ccugaaacag      720

cagaagcagg uucugaccga guucgccaaa gcuguggucg gacucaagac gaaacugaac      780

cuugcccugg gcacagaagu ggacucuucc gaggccaccg cuuggaauuu uagccucggg      840

caacuggaug acaagugggc gggaaucgaa ucugccauga cagaugaggg caccgagauu      900

cuugaccaga uccgggaucu guauagagcu cgccuccuga acgggauugu uccuaccgga      960

aagacacugu cccaggccaa aguggacgac uacacgcagc accacgaaga ucuccaacug     1020

uucaaggccu aucuuaaaca gcuggaggac gauggcaccg cuaaggccau ccggcagcuc     1080

uacgacagau auauugaugg cgacgaugcg gcuccguuuc ugcgcgaaaa uuucgucaaa     1140

gcccuuacaa aggacgugac cgcccauccc aacacaaaga gcccugagcu gcucgaacgg     1200

cuggcucaac ccgauuuuau gcugaaacag agaaauaagg ccaacggggc uaucccugug     1260

cagaugcagc aacgcgagcu cgaccagauc auuaaaaacc aggccgucua cuaugauugg     1320

cuggcggccc ccaauccggu ugagaagcac cggaaaucua ugccuuacca gcuugacgaa     1380

cugcucaacu ucagaauccc cuacuaugug ggaccucugg ugaccgcuaa ggagcaaaag     1440

gccgcucagg gcggggucuu ugccuggaug gugcgcaaag aucccgaagg aaauauuacg     1500

cccuacaacu ucgacgagaa gguugaccgg gaagcguccg ccaauacauu uauccagaga     1560

augaaaacca ccgauacaua ucugauuggc gaggacgugc uuccuaagca gagccuccug     1620

uaccaacggu augagguccu gaacgaacuc aauaacgugc gcguuaauaa cgagaaacug     1680

uccaucgaac agaagcaaca ggugaucaga gagcuuuucg agcggcauaa uucugucacc     1740

auuaagcaau uugcugaaaa ccugagagcc cacggggauu acgcucacau cccggagauu     1800

cgcggacucg ccgacgaaaa acgguuccug agcucccuga gcacauauag acagcuuaag     1860

ucucuccugc ccgaggccau cgaugacccu gcuaaacagg cggauauuga aaauaucauu     1920

gccuggucca cgguguuuga ggacgcugcc aucuucaaga ccaaacugaa ggagaucagc     1980

uggcucggcu cugaagccau uacaaaacug agcaacaucc gcuacagagg cugggggcaa     2040

uucucccgga aguuucuuaa uggacugacc cucggcaacg ggcauacaau uauccaggag     2100

cugcugcuuu cuaccaacaa ucucaugcag auucugacgg augaaacccu gcagaagaaa     2160

augacagagc ucaacgcuga caagcugaaa accgccaaua ucaacgaugc gaucgacaau     2220

gcuuacacaa gccccuccaa caagaaagcc cuucgccaag uccugagagu gguugaugac     2280

auuaagcggg ccgcugacgg acaggauccu agcuggcucu auguggaaac cgccgacggc     2340

gggggcacgc ccggaaagcg cacaagagcg cggcagcacc agcugcaaga gaucuacgcu     2400

aaugccgccc augagcugau ugauaccgcu guccgcggcg aacuugagga caaaaucucu     2460

gauaaggccg acuucaacga uagacucgug cuguauuuua ugcaaggggg acgggacauu     2520

uacacaggcg cuccgcugaa uaucgaucag cucuccagcu augacaucga ucacauucug     2580

ccucagucuc uuaucaaaga caacucccug gacaaucgcg uucucgugca ugccauuauc     2640

aacagagaaa agaaugcgac cuucgccagc accauuuacg cucagaaaau gaacgccaca     2700

uggcggcagu ggcacgaggc ugggcugauc uccggaagaa agcugcgcaa ucuucagaug     2760

cggcccgauc agaucgacaa guaugccucu ggcuuugucg ccagacagcu gguggaaacg     2820

cgccagauua ucaaacucac cgagcagauu guggcugcgc aauacccuga uacaaagauc     2880

auugccguua aagcugggcu gagccaccag cuccgggagg aacuggaguu ccccaagaac     2940

agagacguca accauuacca ccaugccuuu gaugccuucc uugcugcccg caucggcacc     3000

uaucugcuca aacgguuucc uaaucugcag gcguucuuua cauacggaaa guucaccaag     3060

gcugacguga aaaagcugag aggcuuuaac uucauucgcg auaucacgca cgccgaagac     3120

aaaaucgugg ccaaggauac aggggagguc gugugggaca aacagcggga uguugacgag     3180

cuugauagaa uuuauaauuu caagcggaug cugaucaccc acgaagugcg cuuugagacc     3240

gcugaccucu ucaaacaaac agucuacgga gccagagacu ccaaggaagc gggcgggucu     3300

aagcagcuga uucccaaaaa gaaaggauau ccgguggaua ucuacggcgg guauuuucgg     3360

gagaacaccg cuuaccucgc cguugugaag gucacgaaaa agacagaaac cauuuucaag     3420

gugguuaaaa ucgccacaag ccagguggcu gcccugaaua aggcucgcuc cagaagcacc     3480

gccgaggagc uuucuguccu gacagaacuc cugaaaccua aguuuuccaa agugggcaag     3540

aacggaaaga ucaccgacac gcccuucgag gugguccugc cccggguucc uagagaacag     3600

cuguuuuaca augcgaaaua uggcuucuuu auggugaaca gcgauaccau guuccauaau     3660

uuucaagaga uuuggguguc ucgcuccgac cagaagaucc ugcagcagau ucggaaagcc     3720

aagaucgauu acccgaacgu cgaccaagau cucgacaauc uguuccacaa ccucgcugau     3780

cagauuguga aauauuucga ccuguacagc aucgccgggu uuaaggaaaa gaucucucag     3840

agccaaaaua cauucaacga ucuugcuguu gacgauaccg accaguccgu gggaaaaauu     3900

acagucauca augagcugcu gaagggcgcc caggccaacg ggaugaccgg aagccucaaa     3960

gugcugaaga uuucuacacc cuuuggcuuc acccaggaca aauccggggu ucuuacgaag     4020

gauagcucca ucauuuauca aucuccuaca ggccuguuug agagaagcgu gcgccucacc     4080

gaccuguga                                                             4089


<210>  4231
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4231
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uuggcagcaa cuccaucggg       60

uucgccgcug ucgaugaaaa uucucaccuc auuagacuga agggaaaaac aguuaucggc      120

gcccggcuuu uugaagaggg gaaggcggcu gccaaacgca gagccggacg gacgacccgc      180

agacggcuga gccgcaacag auggcggcuc uccuuccugc gcgacuuuuu cgaaucucau      240

auuacaccua ccgauccgaa uuucuucaug agacagaagu auagcgagau cucccccaag      300

gacaaagcuc gguacaagua ugaaaaacgc cuuuuuaacg auagaacaga cgccgaguuc      360

uaccaacagu auucuaccau guaccaucug cggaaucgcc ucaugacaga uccuagcaga      420

gcugacgugc gggagaucua uuuugccauu caccacaucc ugaaguccag aggccauuuc      480

cugacccccg gcgaugcgaa agacuuuaac acgaaugaug uggcccucga cgaaauuuuc      540

ccugcucugc aggaugccua cgcucagguc uaccccgacc uugggaucac cuuugacgag      600

gauaaggaaa acgaguucaa gacaauucug cucaaugaga ucgccaccag cauugacaca      660

caacgcgcgc uggugaaacu ucugcucgcc gaagaggaug acaaggauau ccugaaacag      720

cagaagcagg uucugaccga auuugcuaaa gccguggucg gacucaagac gaaacugaac      780

cuugcucugg gcacagaggu ggacucuucc gaagccaccg ccuggaauuu uagccucggg      840

caacuggaug acaagugggc uggaaucgag ucugcgauga cagaugaggg caccgaaauu      900

cuugaccaga uccgggaucu guauagagcc cgccuccuga acgggauugu uccuaccgga      960

aagacacugu cccaggcuaa aguggacgac uacacgcagc accacgagga ucuccaacug     1020

uucaaggccu aucuuaaaca gcuggaagac gauggcaccg ccaaggcuau ccggcagcuc     1080

uacgacagau auauugaugg cgacgaugcc gcgccguuuc ugcgcgagaa uuucgucaaa     1140

gcucuuacaa aggacgugac cgcccauccc aacacaaaga gcccugaacu gcucgagcgg     1200

cuggcccaac ccgauuuuau gcugaaacag agaaauaagg cuaacggggc caucccugug     1260

cagaugcagc aacgcgagcu cgaccagauc auuaaaaacc aggcugucua cuaugauugg     1320

cuggccgcgc ccaauccggu ugaaaagcac cggaaaucua ugccuuacca gcuugacgag     1380

cugcucaacu ucagaauccc cuacuaugug ggaccucugg ugaccgccaa ggaacaaaag     1440

gcugcccagg gcggggucuu ugcuuggaug gugcgcaaag aucccgaggg aaauauuacg     1500

cccuacaacu ucgacgaaaa gguugaccgg gaggccuccg cgaauacauu uauccagaga     1560

augaaaacca ccgauacaua ucugauuggc gaggacgugc uuccuaagca gagccuccug     1620

uaccaacggu augaaguccu gaacgagcuc aauaacgugc gcguuaauaa cgaaaaacug     1680

uccaucgagc agaagcaaca ggugaucaga gagcuuuucg aacggcauaa uucugucacc     1740

auuaagcaau uugccgagaa ccugagagcu cacggggauu acgcccacau cccggaaauu     1800

cgcggacucg cugacgagaa acgguuccug agcucccuga gcacauauag acagcuuaag     1860

ucucuccugc ccgaagccau cgaugacccu gccaaacagg cugauauuga gaauaucauu     1920

gcguggucca cgguguuuga ggacgccgcu aucuucaaga ccaaacugaa ggaaaucagc     1980

uggcucggcu cugaggccau uacaaaacug agcaacaucc gcuacagagg cugggggcaa     2040

uucucccgga aguuucuuaa uggacugacc cucggcaacg ggcauacaau uauccaggaa     2100

cugcugcuuu cuaccaacaa ucucaugcag auucugacgg augagacccu gcagaagaaa     2160

augacagaac ucaacgccga caagcugaaa accgcuaaua ucaacgaugc caucgacaau     2220

gcguacacaa gccccuccaa caagaaagcc cuucgccaag uccugagagu gguugaugac     2280

auuaagcggg cugccgacgg acaggauccu agcuggcucu auguggagac cgcugacggc     2340

gggggcacgc ccggaaagcg cacaagagcc cggcagcacc agcugcaaga gaucuacgcg     2400

aaugcugccc augaacugau ugauaccgcc guccgcggcg agcuugaaga caaaaucucu     2460

gauaaggcug acuucaacga uagacucgug cuguauuuua ugcaaggggg acgggacauu     2520

uacacaggcg ccccgcugaa uaucgaucag cucuccagcu augacaucga ucacauucug     2580

ccucagucuc uuaucaaaga caacucccug gacaaucgcg uucucgugca ugcuauuauc     2640

aacagagaga agaaugccac cuucgcgagc accauuuacg cccagaaaau gaacgcuaca     2700

uggcggcagu ggcacgaagc cgggcugauc uccggaagaa agcugcgcaa ucuucagaug     2760

cggcccgauc agaucgacaa guaugcuucu ggcuuugucg ccagacagcu gguggagacg     2820

cgccagauua ucaaacucac cgagcagauu guggccgcuc aauacccuga uacaaagauc     2880

auugcgguua aagccgggcu gagccaccag cuccgggagg agcuggaguu ccccaagaac     2940

agagacguca accauuacca ccaugcuuuu gaugccuucc uugccgcucg caucggcacc     3000

uaucugcuca aacgguuucc uaaucugcag gccuucuuua cauacggaaa guucaccaag     3060

gcggacguga aaaagcugag aggcuuuaac uucauucgcg auaucacgca cgcugaagac     3120

aaaaucgugg ccaaggauac aggggagguc gugugggaca aacagcggga uguugacgaa     3180

cuugauagaa uuuauaauuu caagcggaug cugaucaccc acgaggugcg cuuugaaacc     3240

gccgaccucu ucaaacaaac agucuacgga gcuagagacu ccaaggaggc cggcgggucu     3300

aagcagcuga uucccaaaaa gaaaggauau ccgguggaua ucuacggcgg guauuuucgg     3360

gaaaacaccg cguaccucgc uguugugaag gucacgaaaa agacagagac cauuuucaag     3420

gugguuaaaa ucgccacaag ccagguggcc gcucugaaua aggcccgcuc cagaagcacc     3480

gcugaggaac uuucuguccu gacagagcuc cugaaaccua aguuuuccaa agugggcaag     3540

aacggaaaga ucaccgacac gcccuucgaa gugguccugc cccggguucc uagagagcag     3600

cuguuuuaca augccaaaua uggcuucuuu auggugaaca gcgauaccau guuccauaau     3660

uuucaagaaa uuuggguguc ucgcuccgac cagaagaucc uucagcagau ucggaaagcg     3720

aagaucgauu acccgaacgu cgaccaagau cucgacaauc uguuccacaa ccucgccgau     3780

cagauuguga aauauuucga ccuguacagc aucgcugggu uuaaggagaa gaucucucag     3840

agccaaaaua cauucaacga ucuugccguu gacgauaccg accaguccgu gggaaaaauu     3900

acagucauca augagcugcu gaagggcgcu caggccaacg ggaugaccgg aagccucaaa     3960

gugcugaaga uuucuacacc cuuuggcuuc acccaggaca aauccggggu ucuuacgaag     4020

gauagcucca ucauuuauca aucuccuaca ggccuguuug aaagaagcgu gcgccucacc     4080

gaccuguga                                                             4089


<210>  4232
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4232
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uuggcagcaa cuccaucggg       60

uucgccgcug ucgaugaaaa uucucaccuc auuagacuga agggaaaaac aguuaucggc      120

gcccggcuuu uugaagaggg gaaggcggcu gccgaccgca gagccagccg gacgacccgc      180

agacggcugu cccgcaacag auggcggcuc ucuuuccugc gcgauuucuu cgaaagccau      240

auuacaccua ccgacccgaa uuucuucaug agacagaaau auuccgagau cucucccaag      300

gauaagagcc gguacaaaua ugaaaagcgc cuuuuuaacg acagaacaga ugcugaguuc      360

uacaaauaug agaauugccc uaccauguac caucugcgga accgccucau gacagacccc      420

uccaaggccg augugagaga aaucuauuuu gcuauucacc acauccugaa aagccgggga      480

cauuuccuga ccccuggcga cgccaaggau uuuaauacga acaagguggc ggucaauaaa      540

auuuuccccg cccuccaaga cgcuuacgcc cagguguacc cugaucugga caucaccuuu      600

gacgagaaca agguuaauga auuuaaaaca gugcuucuga acgagaaggc uaccccgucu      660

gauacacaga gagcccucgu caaucugcuu cuggcgaaag agggcgaccg cgauauucuc      720

aagcagcaaa aacaggcccu gaccgaauuu gcuaaggccg ugguugggcu gaagacgaaa      780

cucaacgugg cucugggaac agaggucgac uccagcgaag ccaccgccug gaauuuuucu      840

cuuggccagc uggaugacaa guggacaggg aucgaguccg cuaugaccga ugaaggaacc      900

gagauucucg accagauccg ggaucuguau agagcgcgcc uucugaacgg caucgugccc      960

gccgggaaaa cacucagcca agcuaaggug gacgauuacg cccagcacca cgaggaccug     1020

gaacuguuca aagacuaucu caagcagcug gaggaugacg gaacggccaa ggcuauucgg     1080

cagcuuuacg auagauauau cgacggcgau gacgccaaac cuuuuguccg cgaagauuuc     1140

guuaaggcgc ugaccaaaga ggugacagcu caucccaacg ccaagucucc ugaacuccug     1200

gagcggcuug cccaacccga cuuuaugcug aaacagagaa auaaggcuaa cggcgccauu     1260

ccggugcaga ugcaacagcg cgagcucgau cagaucauua agaaucaguc cgucuacuau     1320

gacuggcugg cugccccuaa ccccguggaa aaacacagaa agagcaugcc uuaccaacug     1380

gaugagcucc ugaauuuccg gauccccuac uauguugggc cccuugugac cgcgaaagaa     1440

cagaaggccg cucagggagg cgucuuugcc uggauggugc gcaaagaccc ugaugggaac     1500

aucacaccgu acaauuucga cgagaagguu gaccgggagg cuuccgccaa caccuuuauu     1560

cagagaauga agacgacaga uaccuaucug aucggagagg acgugcuccc caaacaaucu     1620

cugcuguacc agagcuauga gguccuuaau gaacucaaca augugcgggu caacgauaau     1680

aagcuguccu ucgaccagaa acagcaagug cugggcgagc ucuuugaacg ccauaacacc     1740

guuacaauua agcaguucgu ggauaaucug cuugcgcacg gggacuacga uagaaucccu     1800

gagguccggg gacuggccga cgagaaaaga uuucucagcu cucuguccac cuauaaccag     1860

cugaagggca uucuucacga agcuaucgau gacagcgaua aacagacaga caucgagaac     1920

auuaucgccu ggucuacgau uuucgaagau aaggcuaucu ucaagaccaa acucaaugag     1980

auuagcuggc ugaacgccgg caugaucaau ucccuggcca acauucgcua ccgggggugg     2040

ggacaauuuu cucauaagcu ccugaaugaa cuuacacuga gcaccggcag aacaguguuc     2100

gaggagcucc ugaucuccag ccacaaccug augcagaucc uuaccgacga aguucucaaa     2160

cagaagauug cugagcugaa ugcgcaugaa cugaaagccu cugggaucua cgcugccauu     2220

gacgaugccu auacgucccc cagcaacaag aaggcucucc gccaagugcu gucugucauc     2280

gacgauauua ccaaugccgc gggacaggcu ccuaacuggc uuuacaucga gacagccgac     2340

ggcgaugggg ugcccggcaa aagaaccaau ucccggcagc gcaagaucca ggaaauuuau     2400

gccaacgcug cccaagagcu gaucacagga agcgugagag gcgagcucga agacaaaauu     2460

gcgucccggg cugauuuuac cgaccgccug guucuguacu ucaugcaagg gggaagagau     2520

aucuauacgg gcaagccgcu uaauauugac aaccucucua gcuacgauau cgaccacauc     2580

cugccucagu cccugauuaa agacaauucu cucgauaacc ggguccuggu gcauccccuu     2640

gugaaccgcg agaaggacac aaucuuugcc agcaccagau ucgccaagca gaugaauggg     2700

auguggcggc aguggcacgc ugccggacug auuuccagcc gcaaacucag aaaccugcag     2760

augcggccug augaaaucga caaguaugcu ucuggcuuug ucgccagaca gcugguggag     2820

acacgccaga uuaucaaacu uaccgaacaa aucguugcgg aucaguaccc cgacaccaag     2880

auuaucgccg ugaaagcugg gcuguccagc cagcuccgga aggagcugga uuucccuaag     2940

aauagagagg ucaaccacua ccaucacgcc uuugacgcuu uccucgccgc ccgcauuggc     3000

acauaucugc uuaaacggua ccccaaucug gaaccguuuu ucacguaugg aaaguuuaaa     3060

aagaccgagg ugaaaaagcu caaaucuuuc aacuucauca gagauaugac acaugcuaag     3120

gacaagauug uugcgaaaga aaccggcgag aucguguggg auaaugccuc cgacauuaac     3180

gagcuggauc gcaucuacaa uuuuaagcgg augcugauca cacacgaagu cagauucgag     3240

accgcuagcc uuuuuaaaca aacgcuguau gccgccaaga acucuaaaaa ucgcggggga     3300

agccggcagc ucauuccuaa gaagaaaggc uaccuggugg acaucuaugg gggauacaca     3360

caggaaaccg gcuccuaccu cagcguugug agacugacca agaaagcuau guaugccguc     3420

gugaaggugu cuacacggga cgcggcuaaa cuugccgucg ccaaguccau uagcgagcag     3480

aaggaaaacg agacccugaa aaagaucauu gaugggaaac ucuccaagac aucuaaaaag     3540

ggcaagacga cccaccaacu guucgagauc guucugcccc gcgugggaca gaaaacacuu     3600

uuuaagaaua gcaaauacaa ccaguuccug gucaauuccg acaccuauau gcauaacuac     3660

caggaacucu ggaugcccag agaguaucaa cggaugugga aggauauccu gcucucuaac     3720

cacggcgacg cucagcugga agggcagcuu gaucagauuu uuaaauucau cgugagccaa     3780

guuaauuccu acuuuaaccu guaugacauu aaucaguucc gcaagaagau cgccgaugcg     3840

acagacaaau uugcuaagcu gccuacccau gauacggaca acgccagagg aaaaauugcc     3900

accaucggcc agcuccugau cgggcuucag gcuaaugcca ugcggucuga ucugagaaac     3960

cucgacauua agacaccguu cggacugcuc caaaucggca ccgggauuac acuggaucuu     4020

gacaccagca ucauuuacca gucccccaca ggccuguucg agcgcgaagu gccucugacc     4080

gaccucuga                                                             4089


<210>  4233
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4233
augaaccagg agcccuacgc caucggccug gacauuggga ccagcucccu cggaugggcu       60

aaugccgaua uggacggcua uaucgcgaga gugaaggggc aaacaggacu gggcguccgg      120

cuuuucgaag aggggcagcc ugcugccgau cgcagaucuu uucggacgac ccgcagacgg      180

cugagccgca gacgguggcg ccucagacug cuucgggaaa uuuucgacga gccgaucucc      240

ucuauugauc ccaacuuuuu cgcccgccag aaagcuagcu ccaucucucc uaaggacgau      300

agauuugaaa caaccuacca gcuguucaau gacaaaacag aucaagaguu uuauagcaag      360

uaccccacca ucuaucaccu ccggugggcc cugaugacag agcaucgcaa auucgaccug      420

agagaaauuu accucgcugu ucaccauauc gugaaguauc ggggaaacuu ucugagaucc      480

ggcaauccua agagcuucaa acccucugag cuugauuuuu cccagcgcuu caaggaagug      540

aacggccugu ggcaccucau uuuugacgag gucgccccuc agcugccgac cgauaaucuu      600

gaggaaauca cgaaacuggu gcucgacacc acacggacca gagcggaccg ccagcgggag      660

agcguuaagc ugcugauggc cgcuacagau aaccauaaag acuggaagcc cuaccucacc      720

gaguucacga aggccauucu ggggcuuaaa acaaagcugu accuccugac cggaguggau      780

gucgcuaaag ccgaccaaaa gacauggacc uucucucuug aagauaucga agaccacaga      840

gcggaucugg agaccgccuu ugacgaucgc ucccucgagc ugauugaaaa acugguggac      900

cuccaggcug ccauccagcu gacagagauc augccuagcg gccaguauuu cucucaaucc      960

augauuuacc gguaugauca gcacaaggaa caucuuagau ggcugaaaga cuacgcugag     1020

cagcaaacgg acgccaauaa gcgcaaggcc guucggcucg cuuaugauca cuacaucgaa     1080

ggggacgcga aaggaaagcc cgagauugcc gaugacuuuu auaaagagcu gaacagaauc     1140

cuuaagggcg auaccagcga acuggcuaaa aagauuaauc augagaucgc ccucgaacac     1200

uucaugccua agcagcgcac aaaagagaac ggggugaucc ccuaccagau ucaccagcaa     1260

gaacuggacu ccaucauuga uaaucagaag aaauacuauc aguuucuggg agaggacaac     1320

ccggucgugu cucaucacgg cagccagccu uacaagaucg augagcucgu guccuuccgg     1380

guccccuauu acguuggccc uaugauuacc gccgaccaac agaaaaagac agcugccggg     1440

caguuugcgu ggauggugag aaaggauagc ggagacauca cccccuggaa uuucgaugac     1500

aaaguggacc ggacggcuuc ugccacaaac uuuauccagc gcaugaagac caccgauaca     1560

uaucugauug gcgaagacgu ccuuccccug caaucccuca ccuaccagaa auucauggug     1620

cugaacgagc ugaauaacau cagaauuaau ggggaacuuc ucacacggga gcagaagcag     1680

agaacguaua acgauguuuu uaaaggacau ggcaccguga augucaagag ccugaucaag     1740

ucucuggagu gccacagcga agugacaggg gccauuaaag uugagggacu cgcugacccu     1800

aagcgcuuca acuccgcccu gaccacauac cgggauuuua aaaagauccu uccgguggcu     1860

auugaccaac cccagagaca ggccgauauc gaacgcauca uuaauugguc uaccaucuuc     1920

gaggacgcgg auauuuucaa agccaagcug caagaaaucg acuggcucac ggauacccag     1980

agagaccggc ugagcacaaa acgcuacaga ggcuggggcc ggcuguccgc uaagcugcuc     2040

cugaacauua aggauaccca ggggcugagc aucauggacc agcucuggaa uacacaacau     2100

aacuuuaugc agaucgucca cgagccugac uaugccgaga aaauugaaaa gaccaaugcu     2160

aaaggacuga agcagcagga uaucuucgag acgauuaacg aaauguacac aucuccccaa     2220

aauaaaaagg cccuucgcca gauccuggcg guggucagag acauucagaa ggcccaccau     2280

ggccaggcuc cuucccggau cuuuaucgag gccgcucgcg gggccgaaca aaacccccag     2340

agaacccggc agcgccaaaa acagcucgcc gaucuguucg cugaccaggc gaaggagauu     2400

gugaaugaga caguugccca ggaacugaac gaugagaccg acaacaaagc uagauuuacc     2460

gaucggcuua ugcucuauuu ccugcaaaau ggacaggaca uguacacagg cgaaaagcug     2520

aacaucgaua aacucagcac guaugacauu gaucacaucc ugccgcaguc ucuugugaag     2580

gacgacuccc uggauaauag aguccucgug agcaagguug agaaccugaa aaagaaugac     2640

cauuuugccu ccgaacugua cgcccgcaaa augcagggga ccugggagcu uuggcggucu     2700

gcuggccuca uuagcgccag aaagcugaaa caccuucuca ugcgcccuga ugagaucaac     2760

aaguaugcga caggauucau ccggagacaa cugguggaaa cccgccaggu cauuaagcug     2820

gugacagacg ugcuguccaa ugaguacgau ucugacaaaa ccaagaucau uagcguuaaa     2880

gcuggccucu cccaccagcu gcggauggaa cuggaucuuc ccaaggucag agagcugaac     2940

gacuaccauc acgccuuuga ugccuuccuc gcugugcgca ucgggacgua ucugcucaaa     3000

cgguacccua agcuggagag auuuuucgug uauggacagu accaagucgc gccccagcuu     3060

gaccugcggc gcuuuaauuu cauucaugau cucgugagcg gcaaacagga aaagaucguu     3120

gccgaggaaa caggggagau uguguggaac agagaaaagg acgucucuua uaugaaacac     3180

cuucuguccc uuaagcaucu gcucgugacc cacgagguua aagaggauca cggagcucug     3240

uucaaucaga ccaucuacaa ggccaaaaag gacaacggca augucggggg caagaugaaa     3300

cuccugccua caaagagcaa ccggcccacc gaaaucuaug gaggccauuc uucccaagcc     3360

auugcuuacc uugccaucgu gcgcgugaau gggaaagagc cgagcuacag aguuauggga     3420

gugccuauuc ggauccugga ccagcucaag caggcuaaaa agcaaggcga auaugccgag     3480

agacuggcgc ugaagcaguu uuucacaccc gauuuuacga aagggaaggg aaaaauuguc     3540

ucugacuucg auguggugau cccccacauc ccucuucugc agccggucau ugacgaaauc     3600

agcggccagu cccaucgcuu ugcccucggg accaacacau acuaucggaa ccugcaacag     3660

cucauucuga cccuugcuga ucagaagguu cugaucagca aagagacagg cguggacgag     3720

gaaaagaagc ugguggaggu cuacucucac auuugucagc aagugaugaa auucuuuccc     3780

cucuaugaua gaaauaaguu ccgcgaacug cuuguugacu cccggagcca uuuugagcag     3840

cugccuaucg ugaacagaua cuccgaugcc ggaaaacucg aaaucgcugg caagcgcggg     3900

auucugcagc agaucauggu cggacuccac gccaaugcca cccccggcga ccugaaagag     3960

cuugggguga agacgccuuu cggaaagcug caaguucccg gcgggauuaa acugaccgag     4020

aacgcuaagc ucaucuauga aucuccuaca ggccuguuua aacgggaggu gaagauuacc     4080

acagaauga                                                             4089


<210>  4234
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4234
augaagaaaa aggccgagaa aaucuaccug ggccucaacg uggggaccaa uagcauugga       60

uaugcuguca cagacgaaca guacagacug cuuaaguuca acggcaauga ugccuggggg      120

uccaucguuu uugacgcggc uucuagcaac gaggaucggc gcaaucacag acuggcccgg      180

cgcaaccucg acagacggca acagcgcauu guguggcugc aggaaaucuu cgccaaagag      240

auuuccaagg uggaugacag auuuuucauc cggcuuucug agagcuuucg cuggagagag      300

gauguccagg accgguaugu guucuuuaau gauaaggagu acacggacgu ucaauauaug      360

aaagaauacc ccaccaucca ucaccugauu ugcgagcuca uggauaacaa gacaccucau      420

gacgugcgcc uggucuaucu ggcuugugcc uggcucauca cccacagagg acauuuccug      480

aauaaccuua auguggaaaa acuggaugag auuauggacu uuuccucugu uuaccagaac      540

uucaucaauu acuuuacaga gaacggcuau aagcggccgu ggggagaaau ugacgugagc      600

gcucucuccg agguccugaa acagaagacc gggaucacag ugaagaauaa agaacuucag      660

aacauucugc ucgagggcaa gaaacccgaa aagagcggca ccgaggaguu cccuuuuucu      720

caagauucca ucauccgccu gcuggccggg ggacagugca aacucaagga cguguucugu      780

aaagaagagu accuggaucu uggcagcguc ucucugggga uggacgaaga gaaguucgau      840

gaaauuuccg gaagcaucgg cgaggacuau gaucuccuga uugcgcuuag agggcuguac      900

gacuggucug uucucgccaa ccugcugaau ggacacuccg agacgaucag cauggcuaag      960

gugcagaugu augaacagca cggcaaagau cuccaaauuc ugaaguacuu ugugcggaaa     1020

uauguccccg acaaguacaa cgagguguuc agaaaagcca aggcugauaa uuacguugcc     1080

uauaccggcc auauggacaa ggacacagcg ucccagauca aaaagaaagc caaggcugaa     1140

gaguuuucua aauacgugcu uaagcugaug agcccuauca agcccgagaa aaccgaucuc     1200

caguccuaug aagacaugug cgagcgccug aaacuuaacc gguuccugcc uaaacaaaag     1260

aauacagaua acagagucau uccgcaccag cucuacgaau augagcugau ccagauucug     1320

aaaaaugcca gcguguaccu ccccuuucug aacgacacca auaacgggau cucugaagcu     1380

gauaagguuc uuuccguguu ccgcuauaag auuccuuacu acgucggacc ccugaaugag     1440

gagagcucuu uugccuggau cagccggaaa cagggcaaga ucacgccuug gaacuucaaa     1500

gaaauggugg acgaggaugc cuccgaaaau gcuuuuauua gacgcaugac aaacaagugu     1560

accuaucucc ccacagagga cguccugccg aaagauucuc ugugcuacca aaaguucaug     1620

gugcuuaaug aaaucaacaa ccuccagauu gacgggcuga aaaucccugu uaaggcgaag     1680

cagggaauuu aucaugagcu guuugagaaa aauaagaaag ugaagcggaa agauaucgaa     1740

gaguaccuca uuagaaacaa uuaucuggac aaggauagca ccgaacuuau cuccggcauc     1800

gacgagcaga uucacagcgg gcugucuucc uacugugccu ucuuuaaccu ccugaauaag     1860

aaaauccuga gcgaagacga ugucgagcgc auuaucgagc gggcuucuua ugccgaagac     1920

aaguccagag ugauuaaaug gcuucgcaag gaguaccccg gacucagcga agaugacauc     1980

cgguacaucg ccagaauuaa aauccgggau uucggccgcc uguccaagac cuuccugaca     2040

ggccucaagg ggugcgacgu uucuaaagga uuugauggcc aagacgugga gaugauuagc     2100

auccugcaga cgauguggga aaccaacgau aaucuuaugg agauucuguc cgacaaguau     2160

acauuccucg aggcucugga agccuuuacc caggaguacu auacagggaa aaagaaaucu     2220

cugaaggaaa gacuugauga gaugcggguc agcaacuccg ugagacgccc uguguaccgg     2280

acccucgcgg uugucaagga caucgagaag gcuuucggaa agcccaauaa aaucuuugug     2340

gaaaugacga gaggcgggca ggccgaucaa aagggcaaac gcauuaagag cagaaaacag     2400

cagauccugg accuguauga caaguguaag gaugacgugc acgaucucau guggcagcug     2460

gagucucuug gagaauacgc cgacucccgg cugcaaagcg aucgccucua ucuguacuuc     2520

augcaguuug gcaaaugcgc uuacucuggg gcccauauug gauucgagca gcugauggcu     2580

ggcuccgucg aauaugacau cgaucacauu uacccccaaa gcuaugugaa ggacgauucu     2640

cuuaacaaua aaguucucgu gcugagcgac cugaacggga caaaggauaa uaucuacccu     2700

gucuccccgg agaucagagg aaaaaugcag ggccucugga gcugguggaa ccaugugggg     2760

gccauuucug acgagaagua uaagcggcug auccgcuccg cgcccuuuac cgacgaagag     2820

aaaaugggcu ucauuaauag acagcuuacc gaaacaagcc aguccaccaa guuuaucgcc     2880

gaucugcuca aagagcgguu cccugacgcu gaaguugugu acgucaaggc cggacuggug     2940

ucugauuuuc gccacgaguu cgaccugccc aaaagcagau ccuauaacga ucuucauaac     3000

gcugccgacg cguuccuguc uguuguggcc ggcaaugucu acgauaugcg guuuagcaag     3060

aagugguucc gcauugacga gaaauacucc gugaagguga aaacaaucuu uacgcacgaa     3120

gucaagugua gaggggauau uaucugggac ggaaugccua ugcucgagaa aguugaagau     3180

accgcucgga agaacacagc ccacuucgug aaguaugcug ccuuuaaaac cggcgggcug     3240

uucgaccaaa aucccgugaa gaaaggaaca ggccucaucc cgcugaaggc cgggcuuccu     3300

accgagaaau acggcggaua uaacaaggcu ggcauuaugu uucugauccc cgucagauac     3360

aauauuggga agaaaucuga aauccucauu cugccugugg agcugaugca uggaaagcac     3420

uuccuugagg auaaaguuuu ugcgcgcaac uauacguuca cccggcugga aaauauccuc     3480

ggcaagaaag ugggggaggu cagcuuuccc augggaauga gaccuuggaa gaucaacaca     3540

augcuguccu ucgacggcuu ccgcgugugc cucaccggga caagcggccg gggaaaaugc     3600

cugguugccc agcccgugau gcaguuuucu gcugacgaac auuggagauu cuacgucaag     3660

cgccuugagc gguuugugga gaagauuggc aucaauccgg aucugcucua ugacaaagau     3720

cacgacaagg uuuccaaaga ugaaaaccug aagcuguacg agcuuuacgu ggacaaacug     3780

cagaauacca uuuauaagaa gagagucaac agcccugugc aaacgcuccu gaaagggaag     3840

gaagaguuca aaaagcucuc uaucgaagaa cagugucagg ugcugcuuaa uauucagucc     3900

gucuuuggac gcaugacagg cgggugcgau cugacccuga ucggaggcag cgcccacucu     3960

gccgcuacag gcucccucag caccaccauc uccaacugga aaaagaagua cagcacaguu     4020

cggauuaucg accaaucucc cagcgggaug ugggagaaau ccucugaaaa ucugcuugau     4080

uuccuguga                                                             4089


<210>  4235
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4235
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uuggcagcaa cuccaucggg       60

uucgccgcug ucgaugaaaa uucucaccuc auuagacuga agggaaaaac aguuaucggc      120

gcccggcuuu uugaagaggg gaaggcggcu gccgaccgca gagccagccg gacgacccgc      180

agacggcugu cccgcaacag auggcggcuc ucuuuccugc gcgacuuuuu cgaaagccau      240

auuacaccua ccgauccgaa cuuuuucaug agacagaaau auuccgagau cucucccaag      300

gauaaggcuc gguacaaaua ugaaaagcgc cuuuuuaaug acagaacaga ugccgaguuc      360

uaccaacagu auccuaccau guaccaucug cggaaucgcc uccugacaga ccccagcaaa      420

gcugauguga gagagaucua uuuugccauu caccacaucc ugaagucccg gggacauuuc      480

cucaccagcg gcuacgcgaa agacuuuaac acgaauaagg uggcccugaa cgaaauuuuc      540

ccugcccuuc aggaugcuua cgcucagguc uaucccgacc uggauaucac cuuugacgag      600

aauaagguga acgaguucaa aacaguucuc cuggacgaga aggccacccc uucugauaca      660

caaagagcgc uugugaaucu gcuccuggcc gaggacggcg auaaagacau ucugaagcag      720

cagaaacagg uccucaccga guucgcuaag gccgugguug ggcugaaaau gaaacuuaac      780

guggcucugg gaacggaagu cgauuccagc gaagccacag ccuggaauuu uucucucggc      840

caacuggacg auaagugggc ugggaucgag uccgcgauga ccgacgaagg aacagagauu      900

cuugaucaga uccgcgaccu guaccgggcc agacuccuga acggcaucgu gccggcuggg      960

aaaacccuga gccaggccaa gguggaugac uaugagcagc accacgaaga ccucgagcug     1020

uucaaagauu accuuaagca acuggaagac gauggaaugg ccaaagucau ucgccagcuc     1080

uaugaccggu acaucgaugg cgacgaugcu aagcccuuug uuagagagga cuucgugaag     1140

gcccugacca aagaagugac agcgcauccu aaugcuaagu cucccgagcu ucuggagcgc     1200

cucguccagc cugauuuuau gcugaaacaa cggaacaagg ccaacggcgc cauucccgug     1260

cagaugcagc agagagaacu ggaccaaauc auuaaaaauc aguccguuua uuacgauugg     1320

cucgcugccc cgaacccugu ggagaagcac cgccagagca ugcccuacca gcuggacgaa     1380

cuucugaauu uccggauccc uuauuacguc gggccccucg ugacggcuaa ggagcaaaaa     1440

gccgcgcagg gaggcguuuu ugccuggaug gugagaaagg aucccgacgg gaacaucacc     1500

ccuuauaauu ucgacgaaaa agucgaucgc gaggcuuccg ccaacacauu uauucagcgg     1560

augaagacca cagacaccua ccugaucgga gaggaugugc ugccgaaaca gucucuucuc     1620

uaucaaagcu acgaaguccu gaaugagcug aacaauguga gaguuaacga caauaagcuc     1680

uccuucgauc agaagcaaca ggugcugggc gaacuuuuug agcggcauaa cacggucaca     1740

auuaaacagu ucguggacaa ucugcucgcu cacggggauu augaccgcau ccccgagguu     1800

agaggacugg ccgaugaaaa gcgguuucug agcucucuuu ccaccuacaa ccagcucaaa     1860

ggcauucugc acgaggcgau cgacgauagc gacaagcaga ccgauaucga aaagauuauc     1920

gccuggucua caauuuucga ggacaaggcu aucuucaaaa ccaagcugaa ugaaauuagc     1980

uggcucacag ccggcaugau caacucccug gcuaacauua gauaccgcgg guggggacaa     2040

uuuucucaua aacuucugaa ugagcucacg cugagcaccg gccggacagu guucgaggaa     2100

cugcuuaucu ccagccacaa ccucaugcag auccugaccg acgagguccu gaaacagaag     2160

auugccgaac ucaaugccca ugagcugaag gcuucuggga ucuaugcggc cauugaugac     2220

gcuuacacau ccccuagcaa caagaaagcc cuuagacagg ugcugucugu gaucgaugac     2280

auuaccaaug ccgcuggaca agcccccaac uggcucuaua ucgaaacggc ggauggcgac     2340

gggguuccug gcaagcgcac caauuccaga cagcggaaga uccaggagau uuacgcuaac     2400

gccgcccagg agcugaucac aggaagcguc cgcggcgaac uggaggauaa aauugcuucc     2460

agagccgacu uuaccgaucg gcuugugcuc uauuucaugc aagggggacg cgacaucuac     2520

acaggcgaac cccugaauau ugauaaccug ucuagcuaug acaucgacca cauccucccg     2580

cagucccuga uuaaggauaa uucucuugac aacagagugc ugguccaucc ucucgugaau     2640

cgggagaaag auaccaucuu ugcgagcacg cgcuucgcua agcagaugaa cgggaugugg     2700

agacaguggc acgccgccgg acugauuucc agccggaaac ugcgcaaucu ucaaaugaga     2760

cccgacgaaa ucgauaagua cgcuucuggc uuuguugccc ggcagcuggu ggagacaaga     2820

cagauuauca agcucaccga gcaaaucguc gcugaccagu acccugaugc caaaauuauc     2880

gcggugaagg ccgggcuguc cagccagcuc cgcaaagaac uggacuuccc caagaaccgg     2940

gagguuaacc acuaucauca cgcuuuugau gccuuccuug cugccagaau uggcacauac     3000

cugcucaaac gcuauccuaa ucuggaaccc uuuuucaccu acggaaaguu uaagaaaacc     3060

gaggugaaga aacugaaguc ucuuaaaucc uucaacuuca uccgggacau gacacaugcc     3120

aaggauaaaa uugucgcuaa ggagacgggc gaaaucgugu gggacaaugc gagcgauauu     3180

aacgagcugg acagaaucua uaauuuuaag cgcaugcuca ucacccacga aguucgguuc     3240

gagacagccu cucuguuuaa acagacccuc uacgcugcca agaacagcaa aaauagaggg     3300

ggaucccgcc aacugauucc gaagaaaaag ggcuaucuug uggacaucua cgggggauac     3360

acacaggaaa ccggcagcua ucugucuguc gugcggcuca cgaagaaagc cauguacgcu     3420

guggucaagg uuuccacaag agaugccgcg aaacuggcug uggccaagag cauuuccgag     3480

cagaaagaga acgaaacccu gaagaagauc auugacggga aacuuucuaa gaccagcaaa     3540

aagggcaaaa caacccacca gcuguucgag aucgugcucc cucgggucgg acaaaagaca     3600

cuguuuaaaa auuccaagua uaaccaguuc cucguuaauu cugauacgua caugcauaac     3660

uaucaggaac uguggaugcc ccgcgaguac cagagaaugu ggaaggacau ccuucugagc     3720

aaucacggcg augcccaacu ggaagggcag cucgaccaga uuuuuaaauu caucgugucc     3780

caggugaacu cuuauuuuaa ucuguacgau auuaaccaau uccggaagaa aaucgcugac     3840

gccaccgaua aguuugcgaa acuucccaca caugacaccg auaacgcucg cggaaagauu     3900

gccacaaucg gccagcugcu caucgggcug caggccaaug cuaugagaag cgaccuccgg     3960

aaccuggaua uuaagacccc uuucggacuu cugcaaaucg gcacggggau uacccuggac     4020

cucgacacau ccaucauuua ccagagcccg accggccugu ucgagagaga ggucccccuu     4080

acagaucugu ga                                                         4092


<210>  4236
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4236
augagaggca acuaccacau cgggcuggac auuggaacca gcuccaucgg cugggccgug       60

auugaugaca augcuaaggu caugcgggcc aaagggaaga cagcgaucgg agcucgccuc      120

uauaacgagg gccagacggc cgccgauaga cggcuguucc gcaccacaag acggcgccuu      180

aauagacgga aauggcgccu gaagcuccug gacgaaauuu uugaucccua caucaccccu      240

guugacgcug ccuucuuugc uagacuuaaa caaucuaacc ugagcccgaa ggaucagaau      300

cgggacugga caggguccau gcuuuucccc gaucgcaccg acagaaaguu ucauaaagau      360

uauccuacaa ucuaccaccu gagacauaag cugaugaccg aggaccacca guucgauauu      420

cgcgaaaucu aucucgccau ucaucacauc gugaaauaca gaggaaacuu ucugaauacg      480

acccccgugu cuagcuucuc cgucucugac gugaaguuug acgagcaguu cgaucggcuu      540

aacgaacugu augcggccgu uagcccugac gagcccuuug cuauuaaugg cgauaacucc      600

aaagccaucg agacacuccu gcuuagcgac gaaaccaaga aguucgauaa acaaaagcag      660

ucugcuaaac ugauucucuc cgagacaggc gacaaggccc uggauaaaga aaauaccaag      720

guggcgacgc agaucagcaa agccauccug ggguacaaga cacagcucga cguccugcuu      780

aaguacgaug cuauuaacuc uuccgccugg aaaaucgagc uggacgauga gaauauugac      840

gacacccucc cuggacugcu uagccaacug gaugaaaaca gacaggagau ggugucuauc      900

auucagaauc ucuaugcuca gguuacacug aaccaaaucg ugccgaacgg caugucccug      960

agcgaaucca ugaucgagaa guacaaugcc caccgcgacc aucucaaacu gcuuaagcag     1020

cuggaagaua aaauugacga ucccaagaag aaaaaggggc ucgaggccgc uuaugacgcg     1080

uacgucggga aaaacaugaa gguggccaau caggaugugu uuuaugcuuc ugucaaaaag     1140

aaccuggacg auagcgaccu ugccaagcaa aucgccgauc ugauugaggc ucagaaauuc     1200

augccuaagc agcggaccuc ccagaauggc guuauccccc accaacucca ucagcuggaa     1260

cuggaccaga uuaucgagaa ccagagcaaa uacuauccuu ggcucaccgc cgcgaauccc     1320

aacccgaaga gaauccacca agcuaaauac aagcuggaug aacuuguggc cuuccgcauu     1380

ccuuacuaug uggggccccu ggucacaccu gaggaacagg agaagucugc caaucaggug     1440

uuugcuugga ugaaacggaa gcagcccgga acgaucaccc ccuggaacuu cgaccaaaaa     1500

guugacagaa uggccuccgc uaaucgcuuu auucggagaa ugacaaccaa ggauacauac     1560

cucaucggcg aggacgugcu gccugaugcc agccugauuu augaacgcuu caaaguccuu     1620

aacgagcuca aucuggugaa ggcgaacuac caccggcugg ccccggcuca gaaacaggcc     1680

gcuuauagag accucuuuga aaaucagaag ucuaucagca ccaagaaacu gcaaaacuac     1740

cuuguugauc agggcuauuu cacggccaag cccaaaauua cagggcuguc cgacccugag     1800

cgcuuuaacu cuagccucgg aaccuacauc gauuucaagg aaaucuuugg cgaccaggug     1860

gauaaucccg accggcaaga ugacuacgag aaaauuaucg agugguccac cgucuucgaa     1920

gauagacaga uuuaugaggc gaagcugcgg acacuggacu ggcuuaccga agaccagauc     1980

caggcccuca agacaaaacg cuacaacggg uggggaagac ugagcaagaa acugcucacg     2040

ggcauugugg augagaaugg ggagcggauc aucgacgucc uguggaacac caaucaaaac     2100

uuuaugcaag ugcagucuca ggaauccuuc gcuaagcgga uucaugaggc caaugcuaaa     2160

cagaugaagg uuacaggagu ggaagauauc cuugccgacg ccuauaccag cccucagaac     2220

aagaaagcua uucgccaggu cauccgggug guugaugaca uucagaaggc gaugggcggc     2280

guggccccca caucugucuc caucgaguuu accagaagcg aagaucaaaa uccgcgcaga     2340

acgcaguccc gggagucuca gcugcaaaac accuucaaag uggacgcuaa ggagaucgug     2400

gauagcgacc ucguuacaga acuggagacc gucuccaaau cuaagaaggg gcugacagau     2460

cgccuuuacc ucuauuucac ccagcuggga aaagacaugu acacgggcga cccucugaau     2520

auugaugaac ucagcacaua ugacaucgau cacauucugc cccaggccuu uaucaaggac     2580

gauucccuug acaacagagu gcuggugagc aaagccguca auaacgggaa gucuaauaac     2640

gugccuguua aacucuucgg aggcaagaug ggaggcuuuu ggaaagagcu ggcugaucau     2700

cggcugauuu ccaagcgcaa gcucaaacac cuccugaccg accccgauac aaucgacaag     2760

uacaccauga gaggauucau ccggcgccag cugguggaaa ccagccaagu cauuaaacuc     2820

guggcccaga uccugggcga uaaguaccag aacgacgaua caaaaauuau cgagguuacg     2880

gcgaagauga aucaucagau gagagagaac uuuaaacuua uuaagaaucg ggaagugaac     2940

gacuaucacc acgcugucga cgccuaccug accgccuucg uggggcaaua ucucuacaag     3000

cgcuauccua aacugagacc cuacuuuguu uauggaaauu ucaagaaauu uaacgaggau     3060

caugugucuu uccgguacuu uaauuuccug cacgaacuua uggacgagaa caauaagaga     3120

aucgucgaac cggagacagg cgagguggug cuggaacgcg agccugcuau ugccuacaug     3180

aagcgggucu aucauuuuaa auucaugcuc aucucccagg aaguuuacac cagacgcgau     3240

gcgauguuca accagacaau cuaucccgcu aaggaggcca aucggagaaa acugauugcc     3300

aucaagcacg ggaaacccgu ggacguguac ggaggcuaua gcgggucugu cgaugcuuac     3360

auggccauug ugcgcaucca cgacaagaag ggcgauaaau auaagguugu gggagucccu     3420

augcgggcuc ucgagauucu gaacgccacc caugacaaaa aggauuacga cgcgaaacuu     3480

aagcagaucc uggugccgaa guuuacgaaa guuaagaaaa gcagaaagac aggcgaaauc     3540

accaaaaagg uggaggauuu cgaaauuguc cucgggaaag ugauguaccg ccaacugguu     3600

aucgacggag auaagaaguu uauggugggc uccagcaccu aucuguacaa ugcccggcag     3660

cuuguccugu cugacuccag ccucgacaca cuggcuucca aagggacccu cgauucugac     3720

gccgauagca agagacuggu ggcuguguau gacgagauug ucgaaaaagu ugauaaguac     3780

uucccccuuu augacaaaaa cgccuuucgg uccggacugc acaagggccg cgauaaguac     3840

cuccugcugc cuaauagauc uacauucaac ggggccaaaa aggugcauag cggcaaacgg     3900

gagauccuua acgagauucu gaauggacuc cacgcuaacg cgacgcuggg cgggcucaag     3960

gaccugaaaa ucuccacccc cuuuggaaag augcagggcc agucugggau cccucuuagc     4020

uccaauacaa uuaucugcua ucaaagcccc accggacugu ucgaacaucg cgugucucug     4080

aaggaucucu ga                                                         4092


<210>  4237
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4237
auggagaaca auagauacaa cgugggccug gacaucggga ccagcuccau uggauucgcc       60

gucacagaug aacaggacga gcucguucaa gugaagggca aaaauuauau cggggugcgg      120

cuguuuaagg aaggacagac ggcuucugag cgccggagcu ucagaaccac acgcagacgg      180

cuuaaacgca gaaaguggcg gcugggccuc cuggaagaga uuuuugcccc cuaccuugcg      240

cagggggauc cuuauuuccu ggcucgcaug aaagagucca acaucucucc gagagacgaa      300

cggaagaagu acuauggaag ccuccuguuu uccgaugaca ccgucuucua cgauaaauau      360

cccacagugu accaucugcg ccacgcccuc augaccgagg acagaaaguu ugauauucgg      420

gaaaucuacc uggccguuca ccauaucauu aaauaucgcg gcaauuuccu uaucaacaca      480

cugauuaaug aguuuucuag caaggcugug gacuucaaaa aggaugccga cauccucaac      540

gaucuguuug agcaguucua cauuuccgac aucaaugaca gcuuucaacu uaacaccgcu      600

gauaugaaug aaauuucuga gcugcuccug gacaacucca agacgagacu ggaucggcag      660

agagaaagcc ucaaaaagcu ggccaaaaag gucgacgaua ucucuguggc gaaaaagcac      720

cagaaaaucg ccaccgaguu cuccaaggcu auucuuggca auaaggccaa auucgacguu      780

auccugaacg ugacaaccga uagcucuaau gacugguccu uuaagcucga agaugaggac      840

gcugaugaga aaauugucgu gcugcuugag gaacugaaca caccucagca agaagcccuc      900

aacaucauuc aucaguggua uagccagauc ucccugaaug ggaucgugcc cgacggaaag      960

ucuauuagcg aggcgaugau cuccagcuac gaacugcaca aagagcaccu cgcccugcuu     1020

aagcagcaaa uuaacaauac caaggacaaa aagaaagcuc auggccugca cgaggccuau     1080

aacgaauacg uccaugggaa uaacaauaag aaacucaacc aggcugauuu cuauucuaag     1140

guuaagcaga aucuggaguc cagccuuauc acggacgaaa uucuggagcu caucaacgcc     1200

gaaaauuuua ugccuaaaca acgcacaucu gccaacggag ugauccccca ccagcugcac     1260

cagcaggagc uggaucggau uauccaaaau caggcuaagu acuacccuuu ccucgcggag     1320

accaacccga augaaaagag aagcaaggug gccaaauaua agcuggacga gcuugucgcu     1380

uuucgcgugc ccuacuaugu uggcccucug auuacagcca ccgaucagaa gaaaaccucc     1440

gggaaggagu ucgccuggau ggugcggaaa cagcccggaa gaaucacacc uuggaacuuu     1500

gacgaaaagg ucaacaaaau ggagucugcu aauacguuca uucgccggau gaccacaaag     1560

gauagcuacc ucaucgccga agacgugcug cccgaugagu cccugcuuua ucaaaaauuu     1620

aagguucuca acgaacugaa uaaccugaga gcggacggcc gcaagcucaa agugagcguc     1680

aagcagaaag uguucaagga gcuguuucag cagcaaaaga ccauuacaau cgucaaacuu     1740

cagaauuacc ugaaggcuga augcucuuuc cucaccacgc cggagaucuc cggccugagc     1800

aaaccugagc auuuuguguc uucccuggcc acauauauug aucuuaagaa aaucuucggg     1860

gaagacauug auaaccccgc ccggcagaau gacuucgaga gaaugaucga augggcuacc     1920

auuuuugagg acaagaacau cuacagcgaa aaacucaaug agaucaagug gcugaacgau     1980

cgccagaaga aagagcugaa gaauaaaaac uaccacggau ggggcaagcu cuccgccaaa     2040

cugcuuaagg ggcugcggga cucugaugaa uguagcauua ucgagcauau guggaauacc     2100

caacagaacu ucaugcaggu ucaagcugaa gaggacuuug cccagcggau ucaggaagag     2160

aauaaccagc accucaaggg aaacacacug gaggaagugc uggcggaugc cuauaccucc     2220

ccucaaaaua aaaaggcuau cagacagguc gugaaaguua uugaggacau ccagaaggcc     2280

augggcggga ucgcucccca gaaaauuucu aucgaguuca cacgcgcccc cgaagagaag     2340

ccuagacgga gcguggaaag acaacagcag auugagaaaa ucuacaaguc cgucgcgaac     2400

gaaguggaga augccggagu gaaggaugaa cuucagcgcu uuagcgaccg gaaacaaacg     2460

cucaccgaua aguauuuccu guacuuuaca cagcugggca aagacaugua uaccggcaag     2520

ucuauuaaca ucgaucagau cgggaauuac caaauugacc auauccuccc gcaggcuuuc     2580

auuaaagaug acucccugga caacagaguu cuugucagcg ccgcugugaa uaacggaaag     2640

ucugauaaug ugcccacaaa gaacgaccgc uauaaaguca gagagcacgg ccagcggcug     2700

uacgaucagg ggcucaucuc caagcgcaaa uuuaauaacc ugauuaccga cccugauagc     2760

aucaauaagu ucacggccag aggauuugug aaacggcaac ugguugagac cucucaggug     2820

auucgccuug ucgccgaguu ccucuacaac aaguaucaga gcaaguccga aaucaucgaa     2880

gugagagcua aaaugaauag ccagcugcgg aaggacuuug gccugauuaa aaaccgcaac     2940

guuaaugauu accaccaugc gcucgacgcc uaucugacaa ccuucaucgg gaacuaccuu     3000

uauagacggu accccaagcu gcgcgcuuau uucacauacg gcugcuuuaa aaagguggau     3060

gacucucaac ucaaguacuu caauuuucug cacgauauug aggacgacaa aaagaaaauc     3120

auuaaggaua acgacaccgg agaaauccug uggcauagag augagggcau cgcccgguuc     3180

aaaagaauuu auaauuuuaa guacaugcuu gucacgcacg aggugcugac acgccagggg     3240

gccauguuca agcagaccgu uuauuccgcu gccguggcgg gaagcaaaaa gcucaucccu     3300

gucaaagaag accggcccau ugauaucuac ggcggguaua caaacaauaa cgacgcuuuu     3360

cuggccauug ugaaggugac caaaaagaaa caguacaugg ucguuggaau cccgcaaaga     3420

gccgugguga agcucaauga ugcugccaag cagggcucca aagcguauca gaagguccug     3480

uacaacguga ucaaaccuca guucauuaag aaaaagaagg accgcaaaac cgggaagauc     3540

acacaaaaac uugagaauuu ugaaauuguu gugucuaaga cgcuguaccg gcagcucauc     3600

auugauggcg accagaaaua uaugcuggga agcuccgcuu acaaguauaa cgccaagcaa     3660

cugguccuuu cugaguacgc ccuggaagug cucgauagaa gcaucuucga gaaaaaggag     3720

uauaccacag uugaccugga uaauguguac gacgaaauuc ucgcucaggu cgacaaauau     3780

uuuucccugu acgauaagaa caaauuccgc cagaaacuuc acgagucucg gaagaucuuc     3840

accgaacugc ccaucaauaa ccucuuuagc ggcacaaaac ugcugaaguc cgggaaaaga     3900

gagacccuua augagauucu ggaaggacuc caugccaacg cuacgaagaa agaccucaag     3960

agccugaaau ucucuacacc ucugggcaug auccaguacc ccuccgggau uauccuuagc     4020

gagaacgcca uucuguguua ucaaucuccu accggaaucu uugaacgccg ggugacccug     4080

aaggaucucu ga                                                         4092


<210>  4238
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4238
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uuggcagcaa cuccaucggg       60

uucgccgcug ucgaugaaaa uucucaccuc auuagacuga agggaaaaac aguuaucggc      120

gcccggcuuu uugaagaggg gaaggcggcu gccgaccgca gagccagccg gacgacccgc      180

agacggcugu cccgcaacag auggcggcuc ucuuuccugc gcgacuuuuu cgaaagccau      240

auuacaccua ccgauccgaa cuuuuucaug agacagaaau auuccgagau cucucccaag      300

gauaagagcc gguacaaaua ugaaaagcgc cuuuuuaaug acagaacaga ugcugaguuc      360

uacaaauaug agaauugccc uaccauguac caucugcgga accgccucau gacagacccc      420

uccaaggccg augugagaga aaucuauuuu gcuauucacc acauccugaa aagccgggga      480

cauuuccuga ccccuggcga cgccaaggau uuuaauacga acaagguggc ggucaauaaa      540

auuuuccccg cccuccaaga cgcuuacgcc cagguguacc cugaucugga caucaccuuu      600

gacgagaaca agguuaauga guucaaaaca gugcuucuga acgagaaggc uaccccgucu      660

gauacacaga gagcccucgu caaucugcuu cuggcgaaag agggcgaccg cgauauucuc      720

aagcagcaaa aacaggcccu gaccgaguuc gcuaaggccg ugguugggcu gaagacgaaa      780

cucaacgugg cucugggaac agaagucgac uccagcgaag ccaccgccug gaauuuuucu      840

cuuggccagc uggaugacaa guggacaggg aucgaguccg cuaugaccga ugaaggaacc      900

gagauucucg accagauccg ggaucuguau agagcgcgcc uucugaacgg caucgugccc      960

gccgggaaaa cacucagcca agcuaaggug gacgauuacg cccagcacca cgaggaccug     1020

gaacuguuca aagacuaucu caagcagcug gaggaugacg gaacggccaa ggcuauucgg     1080

cagcuuuacg auagauauau cgacggcgau gacgccaaac cuuuuguccg cgaagauuuc     1140

guuaaggcgc ugaccaaaga ggugacagcu caucccaacg ccaagucucc ugaacuccug     1200

gagcggcuug cccaacccga cuuuaugcug aaacagagaa auaaggcuaa cggcgccauu     1260

ccggugcaga ugcaacagcg cgagcucgau cagaucauua agaaucaguc cgucuacuau     1320

gacuggcugg cugccccuaa ccccguggaa aaacacagaa agagcaugcc uuaccaacug     1380

gaugagcucc ugaauuuccg gauccccuac uauguugggc cccuugugac cgcgaaagaa     1440

cagaaggccg cucagggagg cgucuuugcc uggauggugc gcaaagaccc ugaugggaac     1500

aucacaccgu acaauuucga cgagaagguu gaccgggagg cuuccgccaa caccuuuauu     1560

cagagaauga agacgacaga uaccuaucug aucggagaag acgugcuccc caaacaaucu     1620

cugcuguacc agcgguauga gguccuuaau gaacucaaca augugcgcgu caacaaugag     1680

aagcugagca uugaacagaa acagcaagug aucagagagc uguucgagcg gcauaacucc     1740

guuaccauua agcaguuugc ggaaaaucuc agagcccacg gcgauuacgc ucacaugccu     1800

gagauccgcg ggcuggccga cgaaaaacgg uuccuuagcu cucuguccac auauagacag     1860

cucaagagcc ugcugcccga ggcuaucgau gacccugcca aacaggccga uauugaaaac     1920

aucauugcuu ggucuaccgu guuugaggac gcggccaucu ucaagacaaa acuuaaagag     1980

auuaacuggc ucgauagcca aacgaucacc aagcugucca auauucgcua cagaggaugg     2040

ggccaguucu cucauaaauu ucugaacggc cucacacugg ggaauggaca caccaucauc     2100

caggaacuuu uccugagcaa caauaaccuc augcaaauuc ugacagacga gacccugcag     2160

aagaaaauga cggaacuuaa ugcugauaag cucaaggccg ccaacaucaa ugacgcuauu     2220

gaugacgucu acaccucccc cagcaacaaa aaggcccugc gccaggugcu gcggguuauc     2280

gaggacauua aaagagcggc ugauggccag gacccgucuu ggcucuaugu ggaaacagcc     2340

gaugggggag gcaccccugg gaagcggaca cgcgccagac aacaucagcu gcaggagauc     2400

uacgcuaaug ccgcgcacga gcuuaucgac uccgcugucc ggggcgaacu ggaggauaaa     2460

auugccgaca aggccgauuu uaacgaccgc cucgugcugu auuucaugca gggaggcaga     2520

gauaucuaca ccgggacgcc ccugaauauu gaccaacuua gcucuuauga uaucgaccau     2580

auucucccuc agucccugau caaggacaac agccuggaua aucgggugcu cguucacgcu     2640

aucauuaacc gcgaaaagaa cgccacauuu gcuuccacca ucuacgccca gaagaugaau     2700

gcgacaugga gacaguggca cgaggccgga cugauuucug gccggaaacu uagaaaccug     2760

caaaugcgcc ccgaccagau cgauaaguau gcuagcgggu ucgucgcccg gcagcucgug     2820

gaaaccagac aaauuaucaa acugaccgag cagaucgugg cugcccagua cccugagaca     2880

aagauuaucg ccgucaaggc uggacugucc caucagcuuc gcgaggaacu ggaauuuccc     2940

aaaaaucggg acgugaacca cuaccaucac gcguucgaug ccuuucucgc ugccagaauu     3000

ggcacguauc ugcucaagcg cuucccgaau cugcaagccu uuuucaccua cgggaaauuu     3060

aagaaagcug acguuaagaa acuucggggc uucaacuuca ucagagauau uacacacgcc     3120

gaggacaaga ucguggucaa ggauaccgga gaggugauuu gggacaaaaa gcgcgauguu     3180

gacgaacugg aucggaucua uaauuuuaaa agaaugcuca ucacacauga ggugcacuuc     3240

gaaaaggcgg accuguuuaa acagaccguc uacggcgcua aggacucuaa ggaggccggg     3300

ggcaaaaagc agcugauuag caaaaagaaa ggauauccug uggauaucua cgggggauau     3360

acgcaggaaa caggcuccua ccuuagcguu gugcggcuga ccaagaaggc cauguacgcu     3420

gucgugaaag ugucuacccg cgacgccgcg aagcucgcug ucgccaaauc cauuagcgag     3480

caaaaggaga acgaaacacu gaaugauauc auugacgaga aacucucuaa gaucuccaag     3540

aacgggaaaa ucacccauga acuguucgag guugugcuuc ccagaguggg ccagaagaca     3600

cuguuuaaaa auagcaagua uaaccucuuc cuggucaauu cugauacgua caugcacaac     3660

uaucaggaac uguggaugcc ccgggaguac cagaaaaugu ggaaggacau uagccuuucc     3720

aaguauggag augcccaaac cgaggaccag cuggaucaga ucuuuggcuu cauugugagc     3780

cagguuaauu cuuacuuuaa ccucuaugac aucaaccaau uccgcaaaaa gcugaaugau     3840

gcugccgaca aauuugcuac acucccuaug agagauaccg acgacaugca ggggaagauu     3900

gccacaaucg gacagcugcu uaucggccug caagcgaacg ccgcucgguc cgaucugaga     3960

aaucucgaca uuaaaacccc gcuggggcuu cugcagaucc ccaacggaau uagccucgau     4020

uccgacacgu cuguggucua ccagagcccu accggccugc ucgaacgcga ggugcaucug     4080

uccgaucuuu ga                                                         4092


<210>  4239
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4239
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccaucggc       60

uucgccgugg ucgaugcuaa cucucaucug cuuagacuga aaggggagac agccauugga      120

gcgcggcucu uucgcgaagg ccaaagcgcu gccgacagac gggagucccg cacgaccaga      180

cggcgccugu cuagaacacg guggcgccuu agcuuccuga gagacuuuuu cgccccucac      240

aucaccaaga uugauccgga cuuuuuccuc cggcagaaau auuccgaaau cucucccaag      300

gauaaagauc gcuuuaagua cgagaagaga cuguucaaug accggacaga ugcugaauuu      360

uaugaggccu acccuagcau guaucaucug cgccucagac ugaugaccga cgcuucccag      420

gccgaucccc gggagaucuu ccuugcgauu caccacaucc ugaaaagcag agggcauuuu      480

cucacaccug gagccgcuaa ggacuucaac accgaugaag uugaccugaa acacauuuuu      540

cccgcccuug cugaggccua cgcucaggug uauccugacc uggaacucaa uuucgauucu      600

gcccuggcug acgauuucaa ggccaaacug cucgacgagg augcuaagcc guccgacauc      660

caaaaggccc uggugaaccu ucugcucagc ucugauggcg agaaagaaau ugucaagaaa      720

cgcaaacagg ugcugacgga guuugccaaa gcuaucaccg gccuuaagac aaaauucaau      780

cuggcgcucg ggaccgaagu ugacgaggcc gaugcuucca acuggcaguu uagcauggga      840

cagcuggacg auaagugguc uaauauugaa acauccauga ccgaccaagg cacggagauc      900

uucgagcaga uccaggaacu guaccgggcc agacuccuga acgggauugu gcccgccgga      960

augagccuuu cccaggcuaa ggucgccgac uacggccaac acaaagagga ucuggaacuc     1020

uuuaagacau auaucaaaaa gcugaaugac caugagcuug cgaaaaccau ucgcgggcug     1080

uacgaucggu auaucaacgg agacgaugcu aagccuuucc ucagagaaga cuuugugaag     1140

gcccugacaa aagagguuac cgcccacccc aaugaggugu cugaacagcu gcucaaccgc     1200

augggccagg cuaauuucau gcugaagcaa cggaccaaag ccaacggcgc uauuccuguc     1260

cagcuucagc agagagagcu ggaucaaauc aucgccaacc agagcaagua cuaugacugg     1320

cucgcgaaac ccaauccggu ggaaaagcau cgcgccucca agccuuacca gcuggaugag     1380

cugcuuacau uucggauucc cuauuacgug gggccucuca ucacgcccaa acagcaagcu     1440

gaaagcggag agaacgucuu cgccuggaug guuagaaaga aucccucugg caacauuacc     1500

ccuuacaauu uugacgagaa aguggaucgc gaagcguccg ccaacacauu cauccagcgg     1560

augaagacca cagacaccua ucugauugau gaggacgugc ugccgaaaca gagccuccug     1620

uaccagaagu augaaguccu uaaugagcug aacaauguga gaaucaacaa ugaggcgcuc     1680

ucuacggacc aaaagcagcg gcugauccgc gaaguuuuug agagacacag cuccgugaca     1740

auuaaacagg ucgccgauaa ccugguggcu cacggggacu ucgcccagcg gcccgaaauc     1800

agaggacuug cugaugagaa gcgcuuccuc ucuagccugu ccaccuacca ucaacugaaa     1860

gaaauucucc acggcgccau cgacgauccu accaagcugc augacauuga gaauaucauu     1920

acauggagca ccguuuuuga ggaugccgcu aucuucaaaa caaaacuugc ggaaaucgac     1980

uggcuggauc ccgccacgau uaagaagcuc ucuaacaucc gguauagagg guggggacag     2040

uuuucccgca agcugcugga cggccuuaaa cucggcaaug ggcacaccgu gauucaggag     2100

cugaugcuga gcaaccauaa ccucaugcaa auccuggcug augaaacacu uaaggagacc     2160

augacagaac ugaaucagga caaacucaag accgaugaca uugaggacgu caucaacgau     2220

gccuacacgu cuccuuccaa uaaaaaggcc cugagacagg ugcugcgggu cguggaggac     2280

aucaagcacg cugccaacgg acaggauccc agcuggcuuu auauugaaac cgcggacggc     2340

ccggggacag cuggaaaacg cacccaaucc agacagaagc agauccagac aguuuacgcc     2400

aaugccgcuc aagagcucau ugauucugcc gugcggggcg aacuggagga caaaaucgcg     2460

gauaaggcua gcuucaccga ccgccugguc cucuacuuua ugcaaggggg cagagauauu     2520

uauacgggag ccccucugaa caucgaccag cuuucccacu acgauaucga ccauauucug     2580

ccccagucuc ucaucaaaga cgauagccug gacaaucggg ugcugguuaa cgccacaauu     2640

aaucgcgaaa agaacaaugu guucgcuucc acccuuuuug ccggcaagau gaaagcuaca     2700

uggagaaagu ggcacgaggc cgggcugauc agcggacgga aacuccgcaa ccugaugcuc     2760

agaccugaug agauugacaa guucgcgaaa ggcuuugucg cccggcagcu gguggaaacc     2820

agacagauca ucaaacuuac cgagcagauu gcugccgcuc aauaucccaa uacaaagauc     2880

auugccguga aggccgggcu gucucaucag cuccgcgagg aacuggauuu cccuaagaac     2940

cgggacguua aucacuacca ccaugcuuuu gaugcguucc uggccgcuag aaucggaacg     3000

uaucuucuga aacgcuaccc caagcucacc ccguuuuuca cauauggcga guucgccaaa     3060

gucgacguga agaaauuucg ggaguucaac uuuauugggg cccugaccca cgcuaagaag     3120

aacaucauug ccaaagauac aggcgaaauc gugugggaca aggaaagaga uauccaggag     3180

cucgaccgca uuuacaauuu caaacggaug cugaucaccc augaagucca cuuugauaug     3240

gcggaccuuu ucgaccagac gauuuacgcu gccaaggauu ccaaagagag aggaggcagc     3300

aagaaccuga uccaaaagaa acagggguau cucacaggaa uuuacggcgg guauucuacc     3360

gagcgcggau ccuacgugag caucauccgg accuuuaaga aaggcgcccc uauuuauaau     3420

aucguuaaca uuucuacagc ugacgugagc cugcuguccg auaagcagag agucagcgaa     3480

gccucugcgg acgugcuucu gaaagagcuc aucaagaaga aacuggcuaa gcggaaaucc     3540

ggggaaaaua ccaagagcgg cgccuucgag auuguucucc cccgcguggg aaagaacaca     3600

cuguuuaaga auaagaaaua cggccuuuuc cuggucgaau ccacgaccga guuucauaac     3660

uaucaggagc uguggcuccc cagaaaugau caaaugcuga ucaagucuau ccagagccac     3720

aaaauugaag aguccaaggc cgacuacgaa cuuacacgga uguaccgcaa caucgcuucu     3780

cagguggauc acuauuucgg gcuguacacc auuagaaaau ucagcgagaa gcucacagcc     3840

uccaucaaug acuauggagc ucugccuguu uacaagucug aaaccacgga uggcaaauau     3900

gaggugcuca gcaaccuguu ugaggggcuu ggauccggca ccagcacagg gaaucugacc     3960

gccgucaagc ugcagccgaa cuucggccgg guguaccuca caucuggaau ugugcuggaa     4020

gacgauaccu ccaucauuua ucaaagcccc acaggccuuu uugagagauc ugucaaacug     4080

gcggaccucu ga                                                         4092


<210>  4240
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4240
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccaucggc       60

uucgccgugg ucgaugcuaa cucucaucug cuuagacuga aaggggagac agccauugga      120

gcgcggcucu uucgcgaagg ccaaagcgcu gccgacagac gggggucccg cacgaccaga      180

cggcgccugu cuagaacacg guggcgccuu agcuuccuga gagauuucuu cgccccucac      240

aucaccaaga uugacccgga cuuuuuccuc cggcagaaau auuccgagau cucucccaag      300

gauaaagauc gcuuuaagua cgaaaagaga cuguucaaug accggacaga ugcugaguuu      360

uaugaagacu acccuagcau guaucaucug cgccuccacc ugaugaccca cacacauaaa      420

gccgauccca gagagaucuu ccuugcuauu caccacaucc ugaagucccg gggacauuuu      480

cucaccccug gcgccgcgaa agacuucaac acggauaagg uugaccugga ggacauuuuu      540

cccgcccuua ccgaggcuua cgcccaggug uauccugauc uggagcucac auucgaccug      600

gcuaaagccg augacuucaa ggcgaagcug cucgaugaac aggccacccc gagcgacaca      660

caaaaagcuc uggugaaucu ucugcucucu uccgauggcg agaaggagau cgucaaaaag      720

agaaaacagg ugcugaccga auuugccaag gcuauuacgg ggcuuaaaac aaaguucaac      780

cuggcccucg gaaccgaggu ugacgaagcc gaugcuagca auuggcaguu uucuaugggc      840

cagcuggacg auaagugguc caacaucgag acaagcauga ccgaccaagg gaccgaaauu      900

uucgagcaga uccaggagcu guaccgcgcg cggcuccuga auggaaucgu gcccgccggc      960

augucucuuu cccaggcuaa agucgccgac uacgggcaac acaaggaaga ucuggagcuc     1020

uuuaaaacau aucugaagaa acuuaacgac caugaacugg ccaagacgau uagaggacuc     1080

uacgaucgcu auaucaaugg cgacgaugcu aagccuuucc ugcgggagga cuuugugaaa     1140

gcccugacca aggaaguuac agcgcacccc aacgagguga gcgagcagcu ccugaauaga     1200

augggccagg cuaacuucau gcuuaaacaa cgcaccaagg ccaauggggc cauuccuauc     1260

cagcugcagc agcgggaacu cgaucaaauu aucgcuaacc aguccaaaua cuaugacugg     1320

cuggccgcuc ccaacccggu cgaggcccac agauggaaga ugccuuacca gcuggaugaa     1380

cuucucaauu uucauauccc cuauuacgug ggaccucuga uuacacccaa gcagcaagcg     1440

gagucuggcg aaaacguguu cgccuggaug guccgcaaag accccagcgg gaauaucacc     1500

ccuuacaacu uugaugagaa gguugaccgg gaggcuuccg ccaauacguu cauucagaga     1560

augaaaacaa ccgauaccua ucugaucgga gaagacgugc ucccgaagca gagccugcuu     1620

uaccagaaau augaggugcu gaacgaacuc aauaacgucc gcauuaauaa cgagugccug     1680

ggcacagacc aaaagcagcg gcugaucaga gagguguuug aacggcacuc uuccguuacc     1740

aucaaacagg uggcugauaa ucuugucgcc cauggggacu ucgcgcgcag acccgagauu     1800

cggggacucg ccgaugaaaa gagauuccug agcucucuga gcacauacca ccagcucaag     1860

gagauccugc augaagcuau ugacgauccu acgaaacuuc uggacaucga gaacauuauc     1920

accuggucca caguguuuga ggaucacacc auuuucgaaa caaagcucgc cgagaucgaa     1980

uggcuggacc ccaaaaagau caaugagcug ucuggcauuc gcuaucgggg cugggggcaa     2040

uuuagcagaa aacuucucga uggacugaag cugggcaacg ggcacaccgu uauccaggaa     2100

cucaugcugu ccaaucauaa ccuuaugcag auucuggcug acgagacgcu caaggagacc     2160

augacagaac ugaaccaaga uaaacugaag accgacgaua ucgaggacgu gauuaaugac     2220

gccuacacaa gcccuucuaa caaaaaggcc cuucgccagg uccucagagu ggucgaagau     2280

aucaaacacg cugcgaaugg acaggacccc uccuggcugu ucaucgagac cgccgauggc     2340

acggggacag cuggcaagcg gacccagagc cgccaaaagc agauucagac aguguaugcc     2400

aacgccgcuc aggaacugau cgacucugcc guuagaggag agcucgagga uaaaauugcg     2460

gacaaggcuu ccuuuaccga ucggcuggug cuuuacuuca ugcaaggcgg gcgcgacauc     2520

uacaccggag ccccgcugaa uauugaucag cucagccauu augacaucga ucacauccug     2580

ccucaguccc ugauuaaaga cgacucucuu gauaacagag uccuggugaa ugccacaauc     2640

aaccgggaaa agaauaacgu uuuugcuagc acgcucuuug ccggcaaaau gaaggcgacc     2700

uggcgcaagu ggcacgaggc ugggcugauu uccggaagaa aacuccggaa ucugaugcuu     2760

cgccccgacg aaaucgauaa guuugccaaa ggcuucgugg ccagacagcu ggucgagaca     2820

cggcaaauua ucaagcucac cgagcagauc gcugccgcuc aguacccuaa cacaaaaauu     2880

aucgccguga aggcggggcu gucucaucaa cugagagaag aacuugacuu ucccaagaau     2940

cgcgauguga accacuauca ucacgccuuc gacgcuuuuc uggccgcucg gauuggcacc     3000

uaccuccuga aaagauaucc uaagcucgcc cccuucuuca cguacggaga auuugccaaa     3060

guugauguca agaaauuccg cgaguuuaau uucaucggcg cucugacaca ugcgaagaaa     3120

aacauuaucg ccaaggacac cggggaaauu gugugggaua aggagcggga caucagagag     3180

cuugaucgca ucuauaacuu uaaacggaug cugauuaccc acgaagugua cuucgagaca     3240

gcugaccucu uuaagcagac caucuacgcc gccaaagaua gcaaggaaag aggaggcucc     3300

aaacagcuga uuccgaagaa gcagggguau ccuacacaag ucuacggagg cuauacgcag     3360

gagagcgggu cuuacaaugc ucuggugcgc guugccgaag cggacaccac agcuuaucag     3420

gugaucaaaa uuuccgccca gaacgccagc aagaucgcuu cugccaaucu uaaaucccgg     3480

gagaagggca aacaacugcu caacgagauc gucgugaagc agcuggcgaa gagacggaaa     3540

aauuggaagc ccagcgcuaa cucuuucaaa auuguuaucc cccgcuuugg aaugggcacc     3600

cucuuccaga augccaagua cgggcuguuu auggugaaca gcgacacaua uuacagaaau     3660

uaccaggaac uuuggcuguc ccgggagaac caaaaacugc ucaagaagcu guucagcauu     3720

aaauaugaaa agacccagau gaaucacgau gcccuucagg ucuacaaagc uaucauugac     3780

cagguggaga aguucuuuaa acuguaugau aucaaccaau uccgcgccaa gcucucugac     3840

gcuaucgaaa gauuugagaa gcugccuauu aauacggaug gaaacaaaau cggcaagacc     3900

gagacacucc ggcagauucu gaucgggcuu caggccaaug gaaccagauc caacguuaaa     3960

aaccugggca uuaagacaga ccuggggcuc cugcaagugg gcagcggaau caaacuugau     4020

aaggacaccc agauugucua ccaguccccg ucuggccugu ucaaacgccg gaucccccuc     4080

gcggaucugu ga                                                         4092


<210>  4241
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4241
augggcgaca gaaaguacaa ccuggggcuc gauaucggaa ccagcuccau uggcuucgcc       60

gcuguggacg agaauaacca gcccauccgg gucaaaggga agacagccau uggaguucgc      120

cuguuugaag agggcaaaac ggcggcugau agacgggggu uccgcaccac aagacggcgc      180

cuuucuagac ggcgcuggag aaucaaucug cucaacgaaa uuuuugacgc ccaccuggcc      240

gagguggauc cuaccuuccu ugcucggcug aaggaaagca aucgcuccaa ccucgacccg      300

aaaaagucuu uucaaggaag ccugcuguuc cccgagagaa aggauuauca guuuuacgag      360

gaauauccua caaucuacca ucuccggaaa gcccugaugg agaaggaccg caaauucgau      420

uuuagagaaa ucuaucuugc ugugcaccau auuaucaagu accggggcaa uuuccugaac      480

ggcaccccca ugagauccuu uaaagucgag aauauugagc ucgacacacu guucgaucag      540

cuuaaccagc uguaugccga aaucgugccu gacaaugagc ucgcguuuga ucuggcccaa      600

guugcugacg ugaaggacgu ccugucuagc accacgauuu acaagaugga uaaaaagaaa      660

cagcucguga agaugaugcu gcuucccgcc uccaacaaag cucugcagag cgaaaauaag      720

aaaaucguua cccaguucgu gaacgccauu cucaauuaca aguucaagcu ggacguccuu      780

cugcaagugg agacagaugc ggacuggucu cucaaacuga acgaugaagg ggccgacgau      840

aagcuggagg aguuuaccgg agaccucgau gaaaaccgcc uggagaucau cgaccuucug      900

cagcggcucc acaauugguu cucccugaac gaaauuacaa aagauggcaa uagccuuucu      960

gcugccaugg uggagaagua ugaaaaccau caccaccauc uggggcuccu gaaaaagguc     1020

aucgagaauc acccugacgc uaagaaagcc aaggcccuga aagagaccua cacggcuuau     1080

guuggaaaga cagacgauaa aacccagaac caggacgauu uuuacaaggc gguggaaaag     1140

aaucucgacg auuccccgga cgccaaagag auuaagagac ugauccaacu ugaucaguuc     1200

augcccaaac agcgcacagg ccaaaacggg gcuauuccuc aucagcugca ccagcaggaa     1260

cucgaccaaa ucaucgagaa gcagagcaaa uauuaccccu uucuggccga accuaauccc     1320

aacgugaagc ggagaaagga ugccccguau aaacuggacg agcuuauugc uuucaagauc     1380

ccuuacuacg ucggaccccu cgugaccccu gaggaacagg cccagaaugg cgagaacguu     1440

uuugcgugga ugaaacgcaa ggcugccggc cccauuaccc ccuggaauuu cgaugaaaaa     1500

guggaccgga uggagucugc caacagauuu auccgccgga ugacaacgaa ggauaccuau     1560

cuguucgggg aggacguccu gccugcugaa uccaugauuu accaaaaauu ugugguucuc     1620

aaugagcuga acaaccuuaa gaucaaugga agacaccuga gccucaagga caaacaggau     1680

guguauaacg accuguucaa gcagcagaaa acagucucca ucaaggcccu gcaaaauuac     1740

uaugugacca aaaagaaggc ugccacagcg ccgaccgucg gcgggcuugc cgaucccaaa     1800

aaguuucucu cuagccuguc cacguacauu gacuucaaaa acauguucgg agaacgcgug     1860

aaugauccuc aguuucagga ggaccuggaa cagaucguug aguggagcac aauuuucgag     1920

gaucggggca ucuuuaaggc uaaacuccaa gcccuggggu ggcuuucuga aaagcagauu     1980

cagcaacugg uggcuaagag auauaaagga uggggccggc ucuccaagaa acugcugacc     2040

ggccuuaaga acgccgaggg guacagcauc cucgacgaaa uguggcgcuc uaccggaaau     2100

uucaugcaga uucagagcag acccgaguuu gcggcccuga uccagcaagc uaacgaaaaa     2160

caguucgagg gcaaugaucc ugacaacguc ugggagaaua ucgaaaacau ucugggggau     2220

gccuacacau cuccccagaa uaagaaggcu aucagacagg ugguuaaagu gguccaagac     2280

auugagaagg ccgugggaaa cccgccugaa aagaucgcca uugaguuuac ccgggaagcu     2340

gcggccaauc cccagcgcac acagucccgg cucagaacgc uggagaaacu uuaugagagc     2400

gcugaagagg ugguugaugc cggccugacc gccgaacucg cugaguucaa agagaacaag     2460

cauguccugu ccgacaaaua cuaucuguac uuuacacagc uugggcgcga cguguauacc     2520

ggcgauacaa ucagccucga caagcugaac gauuacgacg uggaucacau ccugccucaa     2580

ucuuucauua aggacgauuc ccucgacaau agaguccuga ccauccgggc cgugaacaau     2640

ggaaaaagcg auaacguucc cgcgaagaug uuuggcaaaa agaugggguc uuucuggcgc     2700

uaucuucugg acaauggaau gauuuccaaa agaaaguaca acaaucucau cacggauccu     2760

gacaacauua gcaaguacgc ucagaaaggc uuuaucaauc ggcagcuggu ggaaaccucc     2820

caagucauca agcugacagc caacauucuu aaugggaucu augacaaaga uaccgagauu     2880

aucgaagugc ccgccaagau gaacucucag augcgcaaaa uguucgaccu gguuaaggug     2940

agagagguca augauuacca ucacgcuuuc gacgccuauc ucacaauuuu uaucggaaac     3000

uaccuguaua agugcuaccc gaaacuccag ccuuauuucg uguacgauaa uuuuaagaaa     3060

uucggcaaca aggaagacau cgggcauaaa cgguuuaacu uccugggcaa gauugagcgc     3120

gagaagaaag uuguggcgcc cgaaaccgga gagauccuuu ggagcaaugu cgcucccaac     3180

gaaacgauua agcagaucaa aaagguguac gauuauaaau uuaugauugu guccagagag     3240

aucacaaccc ggcgcgccga acuguucaau caaacagucu acccuaagaa cuaucacggc     3300

aagcucauuc cgaucaaaga ggacagaccc accgaucugu acgggggaua uucuggcaau     3360

accgacgccu accuggcuau cguugcccuu gaggauaaga aaaaggggaa auauuuuaag     3420

guggugggca uuccuacacg ggucgcugcc aaacuggaaa agcucaagca gcaggacagc     3480

cagcaauacc ugcaggcgcu ccacaaagug aucgcucccc aguucacgaa guccaccaaa     3540

aagggaauua aaaagacaga guuugaaauc guucuggaua aggugcauua cagacagcuu     3600

guccaagacg ggccugugaa aaugaugcug ggcagcucua ccuauaagua caacgccaaa     3660

cagcucguuc uguccgagaa ggcccuucag gugauugcug acgaucgcaa auucgacgaa     3720

acacagaagg augacaaucu gaucgccguc uaugaugaga uccugagcau ugugaaccaa     3780

ucuuucgacc ucuacgauau caauggauuu cggaagaaac ugaacgacaa uagagaucag     3840

uucauugacc uccccgccga gaccaaguau gaaggccgca aaguuguggc ucacgggaag     3900

cgggagauga uccuggaaau ucuuaaagga cugcaugcga acgccgcuuu uggcaaucug     3960

aagccuaucg gguucuccac ggccuuugga cagcuccaag uccccaacgg caucauucug     4020

agcaagaaug ccauccuuau ucaccagucu ccgagcgggc uguucgagag aaaaaucaag     4080

cucuccgauc uguga                                                      4095


<210>  4242
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4242
auggagaagc aggaauacua ucugggccuc gacaugggga ucagcuccgu gggaugggcc       60

gucaccaacg auaaauacga gcugcuuaga aagaaaggca aggacaugug ggggauucgg      120

gaguucgagg aagcugauuc uagcgccgag cgcagaacac accggguuaa ucgcagacgg      180

cgccaaagac agguggcgcg gaugggacug cucaaagagu auuuuuccga cgcuaucgaa      240

aagguggauu cuaacuucuu uauucgcauc gagaauagca aguacuucuu ugaagacaaa      300

gauggcagac uguccucuaa gaacgggauu uucgacgaug ccgacuauaa agaugucgac      360

uacuauacgg aguaccccac caucuuucau cuucggaagg cccugcucga uaucgaggac      420

acaggaaaag aauacgacgu gcgccugguu uaucuggcuc ucgugaauau guucaagcac      480

agaggccauu uucugcuuaa caccgagggc gaugcccuga gcuccgaaca ggucaauaca      540

accuaccagg agcuccugga aauucuugag aacaauuaug acaucagccu ggagucucgg      600

gauauuaagg acgugaucga uauuaucucc agcucugaca acgggcgcaa aaagaaacac      660

gaacaaauuc ucgagcuguu cggaguuaag aaauccgaua agagagcuaa ugaacugcuc      720

aaaugccugu guggccuuag cgugaacgcc aagcugaucu uugacgagaa uuacgaagag      780

aaggucgauc ucugcuucca ugacuuuucu uauacagaua aaaucccuga ccugauggag      840

aaacuuuccg aagaggaaua cuauguggug gagcagcuga aaagcauuua cgacuacucc      900

auccucgcgg ggacccugaa gggauaugau uaccugucuu gugcccgggu cgaacucuau      960

gagaaacaca gagaggaccu gaaacuucug aaggcucucu accgccggua uaagacgaac     1020

gaagaguaca augauauguu caagagcgac aaagaaggca ccuauuccgc cuacguuaac     1080

agcuucaacu cuuccgauag cucugugaag auuaaaaagu acagauggaa uaugaagggg     1140

cgcacaaucg acaaccugua uggaaccauu aaaaaggcuu uuaaagauaa uacagacgau     1200

aaggacgugc agagcaugau ggaguccauc gauaaagagc aguuccuucc gaagcaacug     1260

accggcgcca acggcgucau ccccaaucag gugcacaaga aagaaaugga gcggauucuc     1320

aagaaagcgc agaacuaccu gccuuuucug aaggaaaaag acgagucuga acucacgguu     1380

agcgagagaa uccugcgccu uuucacauuu cagauucccu auuacguggg gccucuggga     1440

gaaaauucca agaccggcug ggucgugcgg agagaagggg gacgcguucu ccccuggaac     1500

aucgaugaga aaauugacau gagcgccaca ucugaggcuu ucaucggcaa ucugauccgg     1560

aagugcaccu aucuguccga cgaaaaggug augccuaaag ccagccuuuc uuacgaaaga     1620

uauugugucc ucaacgagau uaauaacauc ugcauugaug gggagcgccu ggacaccgaa     1680

cugaagcaaa agaucuacac agaguguuuu augaagggaa aacgggugac gagaaagcag     1740

auuugcaauu accucgucgc ucagggcugu aucaaguccg augaacaagu gaccggcauu     1800

gacaucaacg uuaauagcuc ccugucuagc uaugggaaaa uguucgccau cuuuggagag     1860

caucuuaagg aagaugccau ucggaaagug gcugaggaga ucauuuacug gggcacaauc     1920

uucuccgacu cuaagaaaau guuucgcaag caccugacca aguaugucga acaggggauu     1980

cucggagaga gccaggugaa aagaauccug ggcuacaagu ucaaagauug ggggcgguuu     2040

uccaaggcgc ugcuuacacu ccagggcauc gacaaaagca ccggagaacu gcugucucuc     2100

gagaacgcca ugugggagua uucccugaac uucauggaac uuauuaauag cgaugacuuc     2160

acguuuaagg augagcuggc uaacagacgc acaucugcca ucaagucccu cagcgaguuc     2220

accuacgaag accuggaaga uaccuauuuu ucuguuccgg ugaaacggau gauuuggcaa     2280

acaauccugg ucgugagaga gauugagcag aucaugggcu acgcccccaa gaaaguguuc     2340

guugaaaugg cucgcagcga ggaagagaag ggggacaaag gaagaaagga cucccggggc     2400

acccagcuuc ucgaacugua caaaaauauc aagaacacag agaaucauga uuggaagaaa     2460

gagauuagcg aagccaacga gucugggaag cugcgcucca aaaagcucua ucuguacuau     2520

acgcagaugg gaagagacau guacaccggc gaagagauca auauugauau gcuuuaugac     2580

gauagcaugu acgacaucga ucacauuuau ccucggcacu acgucaaaga cgauuccauc     2640

gugaacaauc uggugcucgu caaccgcaga gcgaaucaag acaucaagaa ggaucuguac     2700

cccauuccug acaaaaucau uucuaacccc aaggugcagg aacuguggaa aaugcuucau     2760

aagcagaauc ucaucacaga cgagaaauau aagcggcuga ugagccgcuc cccguuuacc     2820

gagaagcagc ugggggauuu cauugcuaga caacucguug aaacacggca gggcaccaaa     2880

ggaaucgccg agcugcuugg ccagcuguuu aaggacacgg aaauugugua cgccaaagcu     2940

accaacgucu cugauuuccg cagagacaau ggguuuguua agagccggcu cgugaacgau     3000

uuccaccaug cccaggacgc uuaucugaac aucguggucg gaaaugugua cuauacaaaa     3060

uuuaccaaga acccuuggaa uuucaucaag aaagauuuug acaaggauag aaacaaaaau     3120

ggcuacaacc uggccaagau guucgaccgc gauguuguga gaggggacga uacagcgugg     3180

auugccucca aaaaggacac ccaaggaacg aucgagacag uccggaagau gaugcgcaaa     3240

aauacacccc uuaugacccg gaugucuuuc gagcagcacg gcgggauugc uaacgccacc     3300

cuguauagcg cuaagaaagc caagguggaa aauuacaucc cucucaaauc cagcgacauu     3360

agacugucug augugaagaa auacggcgga uuuaccuccg cgacagucgc cuauuacuuc     3420

aucguucauc acaaggaggg caagaaagaa aacacgacca ucgaggcucu cccccuguau     3480

ugcaagcgca aaguggaaaa ugaggagaac gggcuucuga aguacuguga agaggugcuc     3540

ggauauggcg acgucaaaau ucuguuuaau aagcugaaga uccagagccu ucuggauauu     3600

aacggguacc aagcccaccu cucuggaaaa acaggcaauc agcugacccu ccggaacgcu     3660

augaaccugu gcgugcccga aaaguggaca gccuauauca aaaagcugga gaaacuuguu     3720

guggggcaug ucaaggaaca ggagauuucc agcgaggcca auaucgaacu cuacgaccug     3780

cugaccgaua agcaccuuaa cucuguguac gcuaaaagac cuaaugcgua uggccagaag     3840

cugaucgaca accgcucccg guucguuaau cucaaaccgg agagccaaug cgaagccauu     3900

cugcagaucc ucaagcugac gaaaauugga aacaaugaga caaaccuuaa gcugaucggc     3960

gaauccgagc auagcgggaa gaugcugauu ucuaaaaauc ucaaggauac cgacaaaccc     4020

gcuaucauuu gucagagcgu gaccggacug uacgagagaa agaucuauau cuuugaugaa     4080

cagcuugaca aauga                                                      4095


<210>  4243
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4243
augaacaaga gauacaauau cggccuggac auugggacca acagcaucgg augguccgug       60

gucaaugauc agucucaacu cguucggauu aaaggcaagc ugggguaugg agugcgccuu      120

uacgacgagg gccagacagc cgaagauaga cggcuguucc gcacgaccag acggcgccuc      180

aaaagacgga aguggcgccu ggggcuucug agagagaucu uugaacccua uauuacaccu      240

caggacgcua ccuucuuucu ccggcagaaa caaagcaacc uguccccgaa ggaucugcag      300

aagcagcagc cccaaaucgg acucuucaau gaccgccugg auucugacuu ucacuaccag      360

uauccuacaa ucuaccaucu uagauaugcc cugaugaccg agaaacggca guucgaucuc      420

cgcgaaauuu accuggcgau gcaccauauc gugaaguaca gaggccacuu ucuuaacgcu      480

gccgccgcua gcuccuucaa aucuggcgag auugaccugg ccaaggauuu ugcugcccuc      540

aaucagaucu ucgggacaau uuuuagcgag gucggauucu cccugagcga aucuaaccug      600

gacgagauca aauccauucu ccuggauaau aaccuuagcg cgucugugcg gcaaagaaag      660

gcccugcccc ucaucuauca gaccgcugcc uccaaggaca gcgaaaaaca guacaaggcu      720

aucgccacgg agcugcuuaa agcgauucug ggccucaagg ccaaauuuaa ucugaucacc      780

gggauugagg uugaagacgu gaaggcuugg acacugucuu ucaacgccga ggauuucgac      840

gcuaaacucu ccgaucugaa uaucgccgac aacgaugacg ccguccaaau uaucgaaauc      900

cuucgcgcuc uguauagcgg agugcuccug accggcauug uuccugaggg gcaguccauc      960

ucucaggcga ugauugauaa guacgaagcc cauaaggcuc accuuaucau gcugcggagc     1020

auuagagagc agcucuccga caaagagaau aaggcccugg aaaaagccua ugaugacuac     1080

aucgaucacc ccgaggacaa gcaacaugga agccgcgaag acuuuuaugc ugccaucaca     1140

aaagugcuga agaaggauuc ugaggacacc aacauuaucg aaauucucaa acugaucgag     1200

cuugaucagu ucaugccuaa gcagcggacg aaagacaacg gcgcgauucc gcaccagcug     1260

caucaacagg agcucgauca gaucauugaa aaucagaagc aauacuaccc cuggcuggcu     1320

gacaucaacc cuaaugagca gagacagaaa auggcccccu auaagcugga ugaacuuguc     1380

acauuucgcg ugccuuacua ugugggcccc augaucaccg ccgaggaaca guuccaaaca     1440

uccaacaaga aauuugcuug gaugauucgg aaggagccgg gggccaucac cccuuggaau     1500

uucgaccaga aagucgauag acaggcuagc gccaacgagu uuauuaagcg caugaccaca     1560

acggacaccu accuccuggc ggaagauguu cugcccaaac aaucucuccu guaucagcgg     1620

uucgaggugc uuaaugaacu gaacggaauc aagauugacg aucagccuau cacaguggag     1680

cagaagcaag ccaucuuuaa ugacaaauuc augcagcagg cucacgucac cgugaagaac     1740

auucaggccu accucguuag cgagggccaa uaugcuucca gacccaauau cacagggcug     1800

ucugacggag aaaaauuuaa cagcucccug agcaccuacc acgaucuuaa gaaaauuuuc     1860

ggcgacauga ucgaugaccc caauaagcag acggauuuug agaaaaugau ugaauggucu     1920

acaaucuucg aggacgggaa gaucuucgcc gauaagcucc gcgaaauuaa cuggcugacc     1980

gacgagcagc ggcagagacu guccaauaaa cgguaccgcg gauggggcca acucagcacc     2040

aagcugcuua caaaaaucgu ggaucagaac ggccagagaa uuaucgacau ucuguggaac     2100

accaaugaua acuuuaugcg gaucauccau ucugacgauu ucgaccaagc ggucacacag     2160

gccaaucaga ugcuccuggc ugacaacgau acgcaggacg ugauuaauga gcuguauacc     2220

uccccuagca acaagaaagc ccuuagacaa auccuccugg uuguggcuga uauucagaag     2280

gccaugaagg ggcaggcccc ggcucgcauc cugauugaau uugcgcggga gaaacaggcc     2340

aaucccagac ucuccacaca acgcagacgg caggucgaac agguguacca ggagcugaac     2400

aaugaccaac uuaacaagga acuggcugau ccucaguaug ccaaucucuc uaaagaccgc     2460

cuguuccugu acuuuaugca gggaggcaga gauauguaua ccggggagag ccuuaacauc     2520

gaccggcucu ccgaguacga uauugaccac auccugcccc aggccuucau caaggauaau     2580

ucucuggaca accgcguccu cgugagcgcu aaaaugaaua gauccaagag cgaccauguu     2640

ccucuggauu uuacagagga acgguucgga caaaagaugc gcucucagug ggaacagaug     2700

cuuagagccg gccugcucac ccggcgcaaa uauaacaacc ugacgcugaa ucccgacucc     2760

cuuaacaagu acaccaugag cggguuuauu aauagacaac ugguggagac acggcagguc     2820

aucaaacucg cgaccaaccu gcucguggau caguacgcug acgaagaugu ucagcuggug     2880

acagucaagu cuggccuuac ccaccaaaug agagaggacu ucgauuuucc gaaaaaucgc     2940

aaccugaaua acuaucauca cgccuucgac gccuaccuca cggcuuuugu gggacuguau     3000

cugcuuaaga aguacccuaa acuggcccac uauuucgugu acggccagua ucagcagggg     3060

uucaaggcga augaucaaug gcggaacuuu aauuuccuca acggacugag acaggagcag     3120

cagguugacg cugaaacagg cgagcucauu uggcaaaaag aucaugaccu ggccuacaug     3180

aauaagaucu acgauuuuaa aaagauucuu gucacccgcg aagugcacac aaaccauggg     3240

gcccuguaua aucagacccu cuacaaagcu aaggacgaca aggccuccgg acagggcggg     3300

cggcaacuga ucgcugccaa agagaacaga cccaccgcga ucuauggcgg auacagcggc     3360

aagacagugg ccuauaugug ccugguccgc auuacggaga aaaaggggga uuucuaccgg     3420

aucuguggag ugcagaccgc uuggguugac gaacuugagc acauugaaga ugccacaauc     3480

aaacugcaga gacucaccgc ucugcucaca ccucaguuuu cugccaccaa gaagcaaaaa     3540

gacgaggugg ucacgguggu uaagcccuuc gaagugcugc uuccuaaaau ucugaucaac     3600

cagcgcuuuu augauggcgg gcaggagcug acacucgcca gcgcuaccua caagaauaac     3660

gcgcaggagc ugguccuuga caaaaaggcc gugcaaguuc ugaauggaca gcuccccaag     3720

gcugaacugg aggcccaggc caccgugguc uacgaucaga uccucgaacg ggugcaaaaa     3780

uauuucccgc uguacgacau gaacuccuuu agagagaaac uuuucguggg ccgggauaaa     3840

uuuaaugaac ugccuuggca ggaccagugg gagaaccaca agcugauuca ggucgggcaa     3900

aagacaaucc ucgaucgcau ucugaucggc cuucaugcua augccgcgau gggagaccug     3960

aaggcucuca acaugagcac ccccuucggc aagcugcaga aaucuaaugg gauuucccuc     4020

agccccgaga cacagaucau uuaucaaucc ccuacgggac uguucgaaag acggguugcc     4080

cuuaaggauc uguga                                                      4095


<210>  4244
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4244
augaagaacc ugaaagagua cuauaucggc cucgacauug ggaccgccag cgugggaugg       60

gcugucacag augaauccua caauaucccc aaguucaacg gcaaaaagau guggggaguu      120

agacuguuug acgaugccaa aacggcggag gaacggcgca cccagagagg gucucggcgc      180

agacuuaauc ggcgcaagga gagaauuaac cugcuccaag accuguucgc uacagaaauc      240

agcaaggugg auccuaauuu cuuccuucgg cuggacaacu ccgaucucua ucgcgaggac      300

aaagaugaga agcugaaauc uaaguacacc cuguuuaaug acaaagauuu caaggacaga      360

gauuaucaca agaaauaccc gacaauucau caccucauca uggaccugau cgaagacgag      420

ggcaagaaag auauucggcu ucuguaucuc gccugccauu accugcuuaa gaaccgcggg      480

cacuuuaucu ucgaaggaca gaaauuugac accaagaaua gcuucgauaa auccauuaac      540

gaccugaaga uccaucucag agaugaguac aauauugacc uggaguuuaa caaugaagau      600

cugaucgaga uuaucacaga caccacgcuc aacaagacca auaaaaagaa agaacugaag      660

aacaucgugg gcgauacaaa auuccuuaag gccauuucug cuaucaugau uggcagcucc      720

cagaagcugg ucgaccucuu ugaggauggg gaauuugagg agaccacagu gaaaagcguu      780

gacuucucua ccacggccuu ugacgauaag uauuccgaau acgaggaagc ucugggagac      840

acaaucagcc uucugaacau ucucaaaucu aucuaugauu ccagcauccu ggagaaucug      900

cucaaggacg ccgauaaauc uaaggacggc aacaaguaca uuuccaaagc guucgugaag      960

aaauuuaaua agcacgggaa agaucugaag acccuuaagc ggaucauuaa aaaguaucug     1020

cccagcgaau acgccaacau cuuccgcaau aaauccauua acgacaauua ugucgcuuac     1080

acaaagucua acaucaccag caauaaaaga accaaggccu ccaaauuuac aaagcaggag     1140

gauuucuaca aguuuaucaa aaagcaccuc gacacgauua aagagaccaa gcugaacagc     1200

ucugaaaaug aggaucuuaa acugaucgac gaaaugcuca cagauauuga guucaagacc     1260

uuuaucccua agcugaaauc cagcgacaac ggagugauuc ccuaucaacu gaagcucaug     1320

gaacugaaaa agauccuuga caaucagucu aaauacuaug auuuccugaa cgagagcgac     1380

gaguacggca caguuaagga uaagguggaa uccauuaugg aguuucggau cccuuauuac     1440

gucgggcccc ucaauccuga cucuaaauau gcuuggauca agagagaaaa caccaaaauu     1500

acgccgugga acuucaagga uaucguggac cuggauagcu cccgcgagga guuuauugac     1560

cggcugaucg gaagauguac auaccuuaaa gaagagaagg ugcuccccaa ggccagccug     1620

auuuacaaug aguucauggu ccugaacgag cucaauaacc ugaaacuuaa ugaguuccug     1680

aucaccgagg agaugaagaa agcgaucuuu gaagaacucu ucaagaccaa aaagaaaguu     1740

acacugaagg ccgugucuaa ccugcuuaag aaagaauuua aucucaccgg cgauauucug     1800

cuguccggca cagacgggga uuucaagcag ggacucaaca gcuauaucga cuuuaaaaau     1860

auuaucggcg auaaggugga ccgcgacgau uaccggauua aaaucgagga aaucauuaag     1920

cugaucgucc uuuaugagga cgauaagacg uaccugaaaa agaaaauuaa gucugcuuau     1980

aaaaacgacu ucaccgauga cgagaucaag aagauugccg cucucaauua caaagauugg     2040

gggagacugu ccaagcgcuu ucugacagga aucgaaggcg uggacaaaac cacaggggag     2100

aagggaagca ucauuuauuu caugcgggaa uacaaccuua aucucaugga gcugaugucc     2160

ggccauuaca ccuuuacgga agagguugag aaacugaacc cuguggaaaa uagagagcuc     2220

ugcuaugaaa uggucgauga gcuguaccuu ucucccagcg ugaagcgcau gcuguggcag     2280

ucccuccggg uuguggacga gaucaagaga auuaucggca aagauccuaa gaaaauuuuc     2340

aucgaaaugg cccgcgccaa ggaggcuaaa aacucucgga aggaaagcag aaagaacaaa     2400

cugcuggagu uuuauaaguu cgggaaaaag gcguuuauua augaaaucgg agaggagcgg     2460

uacaacuauc uucucaauga aaucaacucc gaggaagaga gcaaauuccg cugggacaau     2520

cuguaccugu auuacaccca acucggcaga uguauguacu cucuggaacc cauugaccuu     2580

gccgaucuga aguccaacaa uaucuaugac caggaucaca uuuacccgaa aagcaagauc     2640

uaugacgauu cucucgagaa ccggguccug gugaagaaaa aucugaacca ugagaagggg     2700

aaucaguacc cuauucccga aaaaguccuu aacaagaaug cuuauggauu cuggaaaauc     2760

cuguuugaca agggccucau cgggcagaag aaauacacaa gacugacccg ccggacaccu     2820

uucgaggaaa gagagcucgc cgaauuuauu gagcgccaaa ucguggagac cagacaggcc     2880

acgaaggaaa cagcuaaccu gcuuaaaaau auuugccagg auuccgagau cguuuauagc     2940

aaggccgaaa acgcgucucg guuccgccaa gaguuugaca uuaucaaaug uagaaccgug     3000

aacgaucugc accacaugca ugacgcuuac cucaauaucg ucgugggcaa cguuuacaau     3060

acaaaguuca ccaagaaccc ccugaauuuu auuaaagaua aggacaacgu gcggagcuau     3120

aaucuggaga acauguucaa auacgauguc gugcgcggau ccuauaccgc cuggaucgcc     3180

gacgacagcg aaggcaaugu gaaggcugcc acaauuaaaa agguuaagag agagcuugaa     3240

gggaaaaacu accgguuuac gcgcaugucu uauaucggaa ccggcgggcu guacgaucag     3300

aaucucauga gaaagggaaa aggccagauu ccccagaagg agaacacaaa uaaauccaac     3360

aucgaaaagu augggggcua caauaaagcu agcuccgccu acuucgcgcu gauugagucu     3420

gacggaaagg ccggccggga gcgcacccuc gaaacaaucc cuaucauggu cuauaaccaa     3480

gagaaguacg ggaauaccga agcuguggau aaauaucuga aggacaaccu ugagcugcag     3540

gauccgaaaa uucucaagga caaaaucaag auuaacagcc ugaucaagcu ggauggauuu     3600

cuuuacaaua uuaaaggcaa gacgggggac ucccugucua ucgccggaag cgugcagcuc     3660

aucgucaaca aagaagaaca gaagcugauu aaaaagaugg auaaguuccu cgugaaaaag     3720

aaagacaaua aggauaucaa aguuacaucc uucgacaaca uuaaggagga ggaacugauc     3780

aaacuuuaua agacccuguc ugauaagcug aauaacggca uuuacagcaa uaaaagaaac     3840

aaucaagcua agaacaucuc cgaagcccuc gacaaauuua aggagaucag cauugaagag     3900

aaaaucgacg ugcugaauca gauuauccuu cuguuccagu cuuauaacaa ugggugcaac     3960

cucaagucca uuggccugag cgcgaagacc ggagucgugu uuauccccaa aaagcucaau     4020

uacaaagagu guaagcugau uaaccagucu aucacaggcc uuuucgaaaa cgagguugau     4080

cugcugaauc ucuga                                                      4095


<210>  4245
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4245
auguacaaga aagacuauac cgugggccug gauaucggga gcacauccgu cggauucgcc       60

gcuauugacg augacuacca gcccaucaga cucaagggca aacggguuau uggggugcgc      120

cuguuugagg aaggaaagac ggccgcggag agacggaacu uccgcaccac aagacggcgc      180

cuuaauagac ggaaauggcg ccugucucuc cuggaagaga ucuuugaucc uuauauggcu      240

aagguggacc cgaccuucuu ugccagacuu aagcaaagca accuguuccc caaagauaag      300

aauaaaaagu uuacaggcuc ccuccuguuc ccuaaaacca cagacucuaa guuuuacgau      360

gaauauccca ccauuuacca ccuucgguau caucucauga acgacagccg caaguucgau      420

cugagagaga ucuuucuugc cauccaccau auugucaaau accgggggaa uuuccugaac      480

uccgcuccua ucgccucuuu uagcacggac aagauuaauc ucguggauga cuucgagacc      540

guuaacgcug ccuaccugca gaucaauucc agcaaccccg ugcagcuuga cacagaucag      600

gcggaccaag ucucucgcau ucugcucgau aauaaacugu ccaaccugga caagcagaaa      660

caggccgcua aggcccucgc ucagaaaagc ggagauaaga agcaagacaa aaucaauaac      720

accacaauua agcagaucug caaagcccug cuuggcuaua aguucgaucu ggcggugguu      780

cucaauaucg acucugauga ccacaacaaa uggaagcugc agcuuuccga ugaaaacauu      840

gacagcagac uggccgagcu caccggcgaa cugaaugagg cucagcugga cauccucaac      900

gugcugucuc ggcuuuacuc ccaaauuacg cugaaugaua ucgucccuag cgggaugaca      960

cucuccgagg ccaugauuga aaaguauaaa gcucauaaga aacaccugaa gaugcuuaaa     1020

gacuuugcca gaucucugag caagaaggau uacggacagg ugauggaggc cuaugcugaa     1080

uacaucggca acaucccgga guccgcgagc ucuaaagccc agaagaaaau uucccaagac     1140

gauuucuaua agaaaaucaa gcgcuaccuc gacgauagca agcuggggca gaaaauugcu     1200

gaacugaucg ccaagaauga guuuaugccc aaacagcgga ccaaccagaa uggagugauu     1260

ccuuaccaac uccaccugaa ggagcuuaac cagaucaucg aaaaucaguc uaaauauuac     1320

cccuggcugg ccgagcucaa cccuaaugaa aagagacgcg gcaaagcuaa guauaagcug     1380

agcgagcugg ucgccuuccg gauucccuac uauguugggc cgcuuaucac acaggaggag     1440

caacagaaaa ccuccaacgc gaauuuugcu uggaugauua gaaagucuga gggacgcauc     1500

accccuugga acuucgacga aaaaguggau cggauugcca cagccaauag auuuaucaag     1560

cgcaugacga ccaaagacac auaccucauu ggcgaggaug ugcugcccgc ucauagccug     1620

cucuaugaac gguucaaggu ccugaacgag cuuaacauga ucagagugaa uaaccaccgc     1680

cuguccguug gccagaagca ggacguguuu gaaaaacucu uccaaaagca uaaaaccguc     1740

aagacacggg agcugaucaa acagcugaug gccagcgggc uuccuaccca gcccagaauu     1800

acgggacucu cugaucccgc uaaguuuaau acaucccugg gcaccuacaa caauuucgcc     1860

aagauccugg gggacgagau uaaccagccu gaccggcaag aagauuuuga gaaaaucauu     1920

gaauggauca ccaucuucga cgauaaggac auucucaaaa caaagcugag cgagcuuucu     1980

uggcuggaug agaaacagau caaugcgcuc auuaacaaga auuacuccgg auggggccgc     2040

cugagcagaa agcugcuuac cgaacucaca aacaaaaagg ggcaguccau cauugaccgg     2100

auguggaaua cgcaaaaauc uuucaucgag aucauuaacg aaccgguguu ugccgagcag     2160

aucaaggaug cuaaucagga ccagcuggau gaagacgauu augagaccgu ucugcacgac     2220

gccuacacaa gcccccaaaa caaaaaggcu auuagacagg ugaucaaagu cguggaugac     2280

auuguccgcg ccgcgggacg ggccccuaag uucaucuccc ucgaguuugc uagagaagac     2340

caggagucuc gcagaaccca gagccggcug ucccgcauca agaaaauuua ugaaacaacc     2400

acgaaggagc uuguggccaa ugaucaaauc agagaggaac ugggcaacgu uaaagaccuc     2460

aacgaucggc uguuccugua cuuuacccag cuuggccgcg acgcuuauac aggggagaga     2520

auuaauaucg augacauuag caccaaguac gauaucgacc acauuuuccc ccgggccuuu     2580

aucaaagaug acucucucga uaacaaggug cuggucaagc gcgccgugaa uaacggaaaa     2640

uccaauaacg uuccucugaa acucuucggc gcuaagauga agcccaucug gaaaaugugg     2700

aaggaccauc agcugauuag caagagaaaa uaugaacacc uuaugacaaa uccggacgcg     2760

aucgauaagu acaaagccaa cggguuuauu aaucggcagc ugaucgagac ccgccaaauu     2820

aucaagcucg cugccaacau ccuggccaau agauacucua cggaagacac aaaaauuauc     2880

gaggugaagg cuaaccugaa ucaucagcuu cggcaguccc ugcaccucua uaagaacaga     2940

caagucaaug auuaccauca cgccguggac ggauaucuga gcgcguucgu gggccaguac     3000

cucuaucacc gcuacccuuc ucugcagagc uauuuuguuu acgggcagua ccaacgguuc     3060

uuugauaaac agguccagga aaacaugaag uucaacagau ucaauuuucu uuaugaccug     3120

accgaucgcg acaacgauca gauuguggug aaauccacag gcgagaucgu cggagaaaag     3180

agcaaacuca uugaacaaau caagugggug uaccgguaua aguucaugcu gguuucucag     3240

gagguguuua ccaaaaccgg cccccuguuc gaccagacaa ucuacccugc uggguccaau     3300

aagagcagac uuauucccau caaaaaggau cgcccuacgg acauuuaugg aggcuacucc     3360

ucuagcaccg augccuauau gucccugauc cggguccccg acaaaaagga agacaaauac     3420

aaggugguug gggugccguc uagaagccuc gccucccuga accgcgcuga gaagcgggga     3480

caugaugccu accucgacca gcugagacaa gucauugcga aaucucagaa gaaagaguuu     3540

cagaucauua ucccuaagcu gcgguaucgc cagcugauca uugauggcgg gcucaaauac     3600

acacugggca gcuccgcuua ucaauacaau gccagacagc uggugcuuag cgaaaagucu     3660

auggagguuc uggugaccga cuucuccaag gucacaaccg aucucgacaa acagaaccaa     3720

aagcugaucg ccguguauga ugacauucuc gauaaaguga aucggugucu gccccuuuac     3780

gacaagaacc aguuucgcaa agcucugaau ggagccagag auaaguucgu ccagcugccc     3840

auccucccua aguuuaacgg caauaaaaag gaagaggcug ggaaaguuga aauucugaac     3900

gagauccuuc ugggacucca cgacgaugcc agcacgggca aucugaagcc gcucgggauc     3960

aaaacacccu ucggaaugcu gcagacccaa ucuggcauua ccauguccag caacgcggug     4020

aucgugaauc agucuccuac agggcuuuuu gagcaucgga uuuaucugaa ggcccugcuc     4080

aacaagcuga auaaauga                                                   4098


<210>  4246
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4246
augaccaagc ugaaccagcc cuacggcauc gggcucgaca uuggaagcaa uuccaucggc       60

uucgccgugg ucgaugcuaa cucucaccuu cuuagacuga aaggggagac agccauugga      120

gcgcggcucu uucgcgaagg ccaaagcgcu gccgacagac gggggucccg cacgaccaga      180

cggcgccugu cuagaacacg guggcgccuu agcuuccuga gagacuuuuu cgccccucau      240

aucaccaaga uugauccgga cuuuuuccuc cggcagaaau auuccgagau cucucccaag      300

gauaaagauc gcuuuaagua cgaaaagaga cuguucaaug accggacaga ugcugaguuu      360

uaugaagacu acccuagcau guaucaucug cgccuccacc ugaugaccca cacacauaaa      420

gccgauccca gagagaucuu ccuugcuauu caccacaucc ugaagucccg gggacauuuu      480

cucaccccug gcgccgcgaa agacuucaac acggauaagg uugaccugga ggacauuuuu      540

cccgcccuua ccgaggcuua cgcccaggug uauccugauc uggaacucac auucgaccug      600

gcuaaagccg augacuucaa ggcgaagcug cucgaugaac aggccacccc gagcgacaca      660

caaaaagcuc uggugaaacu ucugcucucu uccgauggcg agaaggagau cgucaagaaa      720

agaaaacagg ugcugaccga auuugccaag gcuauuacgg ggcuuaagac aaaguucaau      780

cuggcccucg gaaccgaggu ugacgaagcc gaugcuagca acuggcaguu uucuaugggc      840

cagcuggacg auaaaugguc caauaucgag acaagcauga ccgaccaagg gaccgaaauu      900

uucgagcaga uccaggagcu guaccgcgcg cggcuccuga acggaaucgu gcccgccggc      960

augucucuuu cccaggcuaa ggucgccgac uacgggcaac acaaagaaga ucuggagcuc     1020

uuuaagacau aucugaaaaa gcugaaugac caugaacugg ccaagacgau uagaggacuc     1080

uacgaucgcu auaucaacgg cgacgaugcu aaaccuuucc ugcgggagga cuuugugaag     1140

gcccugacca aagaaguuac agcgcacccc aaugagguga gcgagcagcu ccugaacaga     1200

augggccagg cuaauuucau gcuuaagcaa cgcaccaaag ccaacggggc cauuccuguc     1260

cagcugcagc agcgggaacu cgaucaaauc auugcuaauc aguccaagua cuaugacugg     1320

cuggccaagc ccaacccggu ggagaaacac agagcuucua agccuuacca gcuggaugaa     1380

cuucucacau uucgcauccc cuauuacgug ggaccucuga ucacccccaa acagcaagcc     1440

gagagcggcg aaaacgucuu cgcguggaug guucggaaga aucccuccgg gaacauuacg     1500

ccuuacaauu uugacgagaa aguggauaga gaggccagcg cuaacacauu cauccagcgc     1560

augaagacca ccgacacaua ucugauugau gaagacgugc ucccgaagca gucucugcuu     1620

uaccagaaau augagguccu gaaugaacuc aacaaugugc ggaucaacaa ugaggcccug     1680

uccaccgacc aaaagcagag acugauucau gagguuuuug aacggcacag cucugugaca     1740

aucaaacagg ucguggauaa ccuuguugcu cauggagacu ucgcccagcg ccccgagauc     1800

agaggccucg cggaugaaaa gcgguuccug agcucccugu cuacguacca ccaacucaaa     1860

gagauucugc auggggccau cgacgauagc accaaacuuc acgacauuga aaauaucauu     1920

acauggucca ccguguuuga ggaugcugcc aucuucaaga caaagcuggc ugagauugac     1980

uggcucgauc cugccaccau caagaaacug agcaacauca gauaucgcgg auggggccag     2040

uuuucucgga agcugcuuga cggccucaaa cuggggaaug gacacacggu cauucaggaa     2100

cugaugcucu ccaaccauaa ucugaugcaa auccuugccg augagacccu gaaggaaaca     2160

augaccgagc ucaaccagga caaacugaag acagaugaca uugaagacgu gaucaacgau     2220

gcuuacacca gccccucuaa uaagaaagcg cugagacagg uccuucgcgu gguugaggac     2280

auuaagcacg ccgcuaacgg ccaggauccu uccuggcucu auaucgagac ggccgacggg     2340

acaggaaccg ccggcaaaag aacacaaagc cggcagaagc agauccagac cguguacgcu     2400

aaugccgcgc aagaacugau ugauuccgcu guccgcgggg agcuggaaga caaaaucgcc     2460

gauaaggccu cuuucaccga cagacucgug cuguacuuua ugcagggcgg acgggauauu     2520

uauacaggcg cuccccuuaa caucgaccag cugagccauu acgauauuga ccacauccuc     2580

ccgcaguccc ugaucaagga cgauucucug gacaaucgcg uucuugugaa cgccacgauu     2640

aauagagaga aaaacaaugu cuucgcgagc acccuguuug cuuccaagau ggccgccaca     2700

uggcggaaau ggcacgaagc ugggcucauc agcggacgca agcugagaaa ccucaugcug     2760

cggccugaug agaccgacaa auucgcccau ggcuuugugg cucgccaacu uguggagaca     2820

agacagauua ucaagcugac cgaacagauu gccgcggccc aauaucccaa uacgaagauc     2880

aucgcuguua aagccgggcu cucucaccag cugcgggagg aacuggauuu cccuaagaac     2940

agagacguca aucauuacca ccaugcuuuu gaugccuucc uugccgcucg cauuggaaca     3000

uaucugcuca aacgguaccc caagcuggcg cccuuuuuca ccuauggcga guucgccaaa     3060

guggacguga agaaauuuag agaguucaac uuuaucgggg cucucaccca cgccaagaag     3120

aauauuaucg ccaaagauac aggcgaaauu gucugggaca aggagcgcga uauccaggaa     3180

cuggaccgga uuuacaacuu caaaagaaug cuuaucaccc acgaggugca uuuugaaaca     3240

gcugaucugu ucgaccagac gaucuacgcc gcgaaggacu ccaaagagcg cggaggccgg     3300

augagccuca uuccuaagaa gcugggguau cugaccggaa ucuacggcgg guaucaaaca     3360

gagagaggau cuuacguuuc cauuauccgc accuuuaaaa agggcgaaac aauuuauaaa     3420

guggucaaca uccuuaccgc ugaugugagc cugcucucug agaaggaaaa aguuagcaag     3480

acgcuggccg acaucuaccu caaggagcug auugccaaaa agcuguccaa aggcaagagc     3540

gggaaaaaua ucgcuucuga accguucgag auugugcuuc cucgggucgg aaaggauucc     3600

cuguuuaaga acaaaaagua uggccucuuc cugaucgaga gcgccaccga acuucacaau     3660

uaccaggagc uguggcuccc cagaaacgac cagcugcuca uuaaacggau ccaggcgcgc     3720

caaaucgaag aguccaaggc ugauuacgaa cugacaagaa uguaucggaa uauugccugg     3780

caaguggacc auuacuuucg ccuuuauaaa aucagaaagu ucgccgagaa gcugcaggcu     3840

ucuguugagg auuucgccgg gcugcccgug uacaaaaacg aaaccagcga cggaaagguc     3900

gaggugcucg cuuccgugcu gaaacggcuu aauggcgccg cggauaaggg gagaacaaac     3960

ccuaccacag ucaaacugca gucugacuuu ggacgcaccc uccugacgaa uggcauuguu     4020

cucgaagaug acagcuccau cguguaucag ucuccgacag ggcuguucaa gcggagcgug     4080

aaacuugucg aucuguga                                                   4098


<210>  4247
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4247
augaccaagg aguacuaucu gggccucgac guggggacaa acagcgucgg augggccguu       60

acggauuccc aguacaaucu gugcaaauuc aagaaaaagg acaugugggg caucagacuu      120

uuugaaucug cuaacaccgc caaagaucgg cgccugcaaa gagggaaucg gcgcagacuc      180

gagcggaaga agcagcgcau ugaccugcuu caggaaaucu ucagccccga gauuuguaaa      240

aucgauccua cauuuuucau uagacugaac gaaucccggc uccaccugga ggacaagucu      300

aaugauuuua aauauccgcu guucaucgag aaggacuaca gcgauaucga auauuacaaa      360

gaguuuccca ccauuuucca ucuccgcaag caccugaucg aauccgagga gaagcaggac      420

auuagacuua ucuaucuggc gcuccauaac auuaucaaaa cacggggaca cuuucugauu      480

gauggcgacc uucaaucugc uaagcagcug cgcccuaucc ucgauaccuu ccugcugagc      540

cuccaggaag aacagaaucu guccgugagc cuuucugaaa accaaaaaga cgaguacgaa      600

gagauccuga agaauagauc cauugccaaa agcgagaagg ugaaaaagcu caagaaccug      660

uuugaaaucu cugacgagcu ugaaaaagag gaaaagaaag cccaguccgc ugucauugag      720

aauuucugca aguuuaucgu ggggaacaaa ggagauguuu guaaguuccu gcgggugagc      780

aaggaggaac ucgagauuga cucuuucucc uuuagcgaag gcaaauacga ggaugacauc      840

gucaagaauc uggaagagaa agugcccgag aagguuuauc uguucgaaca gaugaaagcc      900

auguacgauu ggaacauccu cguggacauu cuggagacag aagaguauau cuccuuugcu      960

aaggucaagc aguacgagaa acauaagacc aaucuucgcc ugcucagaga uauuauccug     1020

aaauauugca cgaaggacga auacaaccgg auguucaacg augagaaaga agccggcucu     1080

uauaccgcgu acguggggaa acuuaagaaa aauaacaaga aauacuggau ugagaagaaa     1140

agaaauccug aagaguuuua uaagagccug ggaaaacucc uggacaagau cgagccccug     1200

aaggaagauc ucgaggugcu gacaaugaug aucgaagagu guaaaaacca cacccuucug     1260

ccuauucaaa agaauaaaga caacggcguc aucccgcacc agguucauga aguggagcuc     1320

aagaaaauuc uggagaaugc caagaaguac uauuccuucc ugacagaaac cgacaaagau     1380

ggguacagcg ugguccagaa gaucgagucu auuuuucgcu uccggauccc cuauuacgug     1440

ggaccucuuu ccacgagaca ccaagaaaaa ggcagcaacg uuuggauggu gcgcaagccc     1500

gggcgggagg acagaauuua uccuuggaau auggaagaga ucaucgauuu ugagaaaucu     1560

aacgaaaauu ucauuacacg caugaccaac aagugcacau accucaucgg agaggacguc     1620

cugcccaagc auagccugcu cuacuccaaa uauauggugc ugaaugaacu uaacaauguu     1680

aaggugcggg gcaaaaagcu gccgaccucu cucaaacaga aggucuuuga ggaucuguuc     1740

gagaacaaga gcaaagugac cggcaagaac cugcuugaau accuccagau ucaggacaaa     1800

gauauccaaa uugacgaucu guccggguuu gacaaggauu ucaaaacaag ccugaagucu     1860

uaucucgacu ucaaaaagca gaucuuugga gaggaaaucg agaaggaauc cauucagaau     1920

augaucgagg auauuaucaa auggauuacg aucuacggca acgacaagga gaugcugaaa     1980

agagucaucc gcgcuaauua uagcaaccag cuuaccgagg agcaaaugaa gaaaauuaca     2040

ggguuccagu acucuggaug gggcaauuuu uccaagaugu uccugaaggg gaucagcgga     2100

uccgacgugu cuaccggcga aacauuugau auuaucaccg ccauguggga gacggacaac     2160

aaucucaugc agauucugag caaaaaguuc acauuuaugg auaacguuga agacuucaau     2220

uccggcaaag uggggaagau cgauaaaauc accuaugacu cuaccgucaa ggagauguuu     2280

cugagcccug agaacaagcg ggcugugugg cagacaauuc aaguugccga agagaucaaa     2340

aaggugaugg gaugugaacc caaaaagauu uucaucgaga uggcgagagg cggggaaaaa     2400

gucaagaagc gcaccaaauc ccggaaggcc cagcuucucg agcuguacgc ugccugcgag     2460

gaagauugua gagagcugau uaaagaaauc gaggaccggg augagcgcga cuuuaauagc     2520

augaaacucu uccuguacua uacacaguuu ggaaagugca uguacucugg cgaugacauu     2580

gauaucaacg aacuuaucag aggcaauucc aagugggacc gggaccacau uuauccucag     2640

agcaagauca aagaugacuc uauugauaac cuggugcucg ugaauaagac guacaacgcu     2700

aaaaagucca acgagcugcu gagcgaagac auccaaaaga agaugcacuc uuucuggcuu     2760

agccuccuga auaaaaagcu gauuaccaag uccaaauaug auagacucac acgcaagggg     2820

gacuucaccg augaggaacu gagcggauuu aucgcccggc agcuuguuga gacaagacag     2880

ucuaccaaag ccaucgcuga cauuuucaag caaaucuacu ccagcgaggu cguguaugug     2940

aaauccucuc uggucagcga uuuucgcaag aagccccuca acuaccugaa auccagacgg     3000

gugaaugacu accaucacgc gaaggaugcc uaucugaaca uuguuguggg caaugucuac     3060

aacaaaaagu ucacgucuaa ucccauccag uggaugaaaa agaaccgcga caccaauuau     3120

agccuuaaca agguguuuga acaugacguu gugauuaaug gggaggucau cugggaaaaa     3180

uguacauacc acgaggauac caacacauau gacggaggca cccuggauag aauccggaag     3240

auuguggaac gcgacaauau ccucuacacg gaguaugcuu acugcgagaa aggggaacug     3300

uucaacgcca caauucagaa uaagaacgga aacuccaccg uuucucucaa aaagggccug     3360

gaugugaaga aauacggggg cuauuuuagc gccaauacau ccuacuucag ccuuaucgag     3420

uuugaagaca agaaaggaga uagagcucgg cauauuaucg gcgucccuau uuauaucgcc     3480

aacaugcugg agcacucucc guccgcguuc cucgaauacu gcgagcagaa gggguaucaa     3540

aaugugcgca uccuggugga gaaaauuaag aaaaacagcc ugcuuaucau uaauggauac     3600

ccccugagaa uccggggcga aaacgagguc gacaccucuu uuaagcgcgc uauucagcuc     3660

aagcuggauc agaaaaauua ugaacucguu agaaacaucg agaaguuccu ggagaaauac     3720

guggaaaaga aagggaauua cccuaucgac gagaaccggg aucacauuac ccaugaaaag     3780

augaaucagc uuuaugaggu gcugcucucc aagaugaaaa aguucaacaa aaagggaaug     3840

gccgacccca gcgauagaau cgaaaaaucu aagccuaagu uuauuaaacu ggaggaccug     3900

aucgacaaga uuaaugucau caacaaaaug cuuaaucugc uccgcuguga uaacgacaca     3960

aaggccgauc ugucccucau cgagcugccc aaaaaugcug gcagcuucgu gguuaagaag     4020

aacacgauug ggaaauccaa gaucauucuu gugaaccaaa gcgucaccgg ccuguacgaa     4080

aaucggagag agcuguga                                                   4098


<210>  4248
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4248
auggacaccg ccaagacaaa caaacagccc uacuauaucg gccuggauau ggggacgagc       60

uccgugggau gggcugucac cgacgccucu uacaauguug ugaagaaaca cggcaaggcg      120

cucuggggag ugagacuguu cgaggaagcu caaacagccg ccgaucggcg ccuucauaga      180

accgcucggc gccggauuca gagacgcaaa cagagacugg cccuccugca ggagcuuuuu      240

gcuaaggaaa ucugcaaggu cgacccuggg uucuuuauuc ggcugcacga gagccgccuc      300

uggcaagaag auaaauccau cgaccagccg aacacacugu ucaaugauga ggacuuugau      360

gacaaggccu aucauaaagc guaccccacc auuuaucacc ugagauacgc ccucaugaca      420

gagaacaagu cuuucgaugu gcggcugguu uaucuugcua uccaucacau cauuaaacac      480

cgcggccauu uucugcucga aaauuacgac cugagcaacc aggagaccuc cggguucgau      540

gaaucuuaca ucgcccuuac gaaugcuauu aaggagcugc uccagaagga gaucccugug      600

ggacacgucg agaaaauuca aaucauucug aaggacaaaa gccuguccaa gagagagaaa      660

accgaaaaga uccuccaggu gugggacguu aucaaagaua agcagcugaa ggaggugguc      720

cagcuuauuu guggcggcac agccaaccug agcacccucu uuauggacga aucucugaaa      780

gaggcgcuua agguguucga gaucaaagau uccaagauua gcuuuucuac agccguguau      840

gaagagaaag aauccgaccu gcaaagcguc cucgagcagc gguucgaucu gguuuacgcu      900

gccaagaugc uguaugacug gucucuccug gcugaacuua ugggggaucg caagacccug      960

uccgaggcca aaaucgccau uuacgagacg cauaagaaag accucgaagu gcugaagaga     1020

cuuacacggc aggauaaaag ccuguauaga aagcucuuuc gcggaaccga caaggauucc     1080

uacucugcuu augugggcag cugcaugauc cacggggaga aacuggucau cgaaaagcgg     1140

ggauccacag aggaauuuua caaauuccug aagaaagaga uuagcaagcu ccccgacugu     1200

gaggacaaga ucuacgugcu gucuaaaauu gaagcggaga auuuucuucc uaaggccguu     1260

uccaccauca acggcauuau ccccuaucaa cugcaggaac aggagcucga uaucauucug     1320

aaaaaggcug agcaguaccu gccuuuccuu auggaaaaag acgccuaugg gaccaucagc     1380

gagaagauua aacaacuccu gacauuuaga aucccguucu acgugggacc ccugaaucgc     1440

cacucugaua agagcugggc cguccauacg gacaaggaag gccggauucu cccuuggaac     1500

uuuaaugaga gaaucaacga agagaaaucc gcugagaagu ucauuacccg caugacaaau     1560

aaaugcaccu aucugcuugg caaggaugug cugcccaaaa acucucuccu guacacagaa     1620

uauaugcugu uuaacgagcu uaauaacguu aagaucggga aggccggaac cuaccucacg     1680

gaagaacaga aagaaaagcu guggaaagag cuguuccuca caaccaagaa agugacccug     1740

agcaaguuuu ccaaguuccu uaucaaagag ggcauugaca aggaagaggc ggucgaaauc     1800

aaagggcugg auggaggcuu uaagagcucu cucgcuccuu ggauugaccu gaaaaauauc     1860

cugggggagg auuucacacg ggacaaggcc gaagagauua ucaaggccau cacccuuuuc     1920

uccauggaua aaaagauucu caaaaaguac cugaugagaa ucgugagcga gcccgucgcu     1980

aaacagcugu cuaagcuccg cuauacagga uggggccggu ucuccaaaga guuccugacg     2040

gaaauuaccc cgagagugac agacgauguu caggugcuug ucgacaccag cacaggcgaa     2100

aucaugaaca uuaucaccgc ccugaagaag acgucccuca aucugaugga gauccugucu     2160

agcaccuacg gguauuccgc ugccauuucu gaggcgaaca aagaacuugu gggaaauaca     2220

guucucagcu acgagaccgu gaaggaacug acagucuccc cugccgugaa acgccccauc     2280

uggcaaaccc ugaagauuau caaagagauu acgcacauca ugggcgcuga accugcccgg     2340

aucuuuauug agauggcuag agacaagcag cccgauaagg ggcggacaga cagccgcaaa     2400

aagcagcuca ucgagcugua uaaaaagugu aaagaagaug ugagaucuug guccaaccgg     2460

gagagcaagg acuggguuga agagauugaa ucuaaguccg auagccagcu uagaucuaau     2520

aaacuguucc ucuacuauac ccaaauggga aagugcaugu acacaggcaa acccaucgac     2580

cugggggccc ugcuuaaggg ccagcuguac gaucgcgacc auauuuaucc ucagagccag     2640

accaaagaug acucccucga uaaccugguc aucguggaca aucgggagaa cagagauaag     2700

agcaauaccu acccgcucuc uaagaacauu cgcgagagac aaaucggauu uuggaaacug     2760

cuucgggaca agggcuucau cuccaaagaa aaguauuuuc gccugacaag aaauacgccc     2820

uucagcgacg cggagaaagc cgaauuuauu gcucggcagc ucguggagac ccgccagucc     2880

acaaaggccg ucgcugaaau ccugaagaga gugcugccua aaucuauuau cguuuacgug     2940

aaggccgggc uuaccagccg guuccgccaa uauggagagu uuauuaaagu cagagaucug     3000

aacgacuacc accaugccaa ggaugcuuau cucaacaucg ugacaggcaa uguucacuac     3060

accaaauuca cguccaaccc cauccgguuu gugaaggacu gucagauuua cucucugaau     3120

gagcacgcgc ucuauaaaua cacagucgaa cgcaacgggg ugguugccug ggcuccuaau     3180

gaggaaggaa ugaugcccag cgugaccaga uggaugaaca agaauaacau ccuggucacc     3240

cggauguccu augugggcca gggggagcuu uucgaucagc ugccgcucaa gaaaggcaag     3300

ggacaagugu cucugaaaaa ggagggcgcc auuagcgaca ucuccaaaua cgggggauau     3360

aauagcgccu cuacagcuua cuuuauguau gucgaaggcg aggauaacaa ggggaagccu     3420

guuuacguga uugaaaccau gccccugcau cuugccgcga gacugacaac gcucgaggaa     3480

aaacagaagu acugcgagga gcuguggaug uccaccggag gcaaacucaa gaauccuaaa     3540

gugcugaucg aacagauccc cauucagagc cuuuucucug ucgacggguu ccgcgugaca     3600

aucgcuggca agaccggaaa gcaauauggc ugucggcugg ccgaggaacu gguucucaac     3660

aauggguacg ccaugacacu gaaagcugug cuuaaguuug ucaacagacg caaaaugaau     3720

aaggaggccc ugauuaccuc cuaugaucac cucgaagaga gccagcugcu cgcgcuguac     3780

gacguguucc uugauaaacu ggagcauucu ugcuuuucca agcgguucag cacgcagaga     3840

gacguucuga agaacggacg cgaauccuau aucgcucuca gccuggagga caaaugucag     3900

auucuuucug aaauccugca ccucuuucaa ugcaacgcca cccugagcaa ucucucccug     3960

cuuggcucua gcaagcaguc cgggcggcug uacaugucua aaaaccugag cgauaaggac     4020

uccugguaua uuauccacca gucugugaca ggauucuacg agcaacauau cccucucgcc     4080

cccuacaaaa agagcuga                                                   4098


<210>  4249
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4249
auggugaaga aaaaguacgc caucggcauu gacaucggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa agugcccucu aagaaaauga aggucuucgg caauacggag      120

aagagauaca uuaaaaagaa ccugcucggg acccugcuuu uugaugaagg aaauacagcu      180

gagaaccggc gccugaaaag aaccgcccgg cgcagauaua cacggcgcag aaaucggauc      240

cucuaccugc aggaaauuuu cgcggaggaa aucaacaaga ucgacgauag cuuuuuccaa      300

cgccuugacg auuccuuucu gauuguggag gacaaacagg gcucuaagca cccuaucuuc      360

gggacccucc aggaggaaaa gaaauaucau aagcaguuuc cgacaauuua ccaccugaga      420

aaacaacugg cugauagcuc ccagaaggcc gacauccggc ucauuuaucu ggcccuugcu      480

cauaucauua aauaccgcgg acacuuccug uuugagggcg aucucaaguc ugaaaauaaa      540

gacguucagc aucuguucaa cgauuuugug gagauguucg acaagaccgu cgagggcagc      600

uaccuuuccg aaaaucugcc caacguggcc gacguucucg uggagaaggu cagcaaaucu      660

agacggcugg aaaauauccu gcacuauuuu ccuaacgaga agaaaaaugg acucuucgga      720

aacuuccugg cucuugcccu gggccuccag cccaauuuua agacgaacuu cgaacuggcg      780

gaggaugcca aaauccaauu uuccaaggag accuacgaag aggaccuuga ggaacugcuc      840

gggaagauug gagaugacua ugcugaucug uucaucgcca caaaaagccu guacgacggc      900

auucuccugg cugggauccu uucuaccaca gauuccacca cgaaggcccc ucugucuagc      960

uccaugguga accgcuauga ggaacacaaa aaggaccucg cgcugcuuaa aaauuuuauu     1020

caucagaacc ugagcgauuc cuacaaggag guguucaaug acaagcucaa agauggauau     1080

gccggcuaca ucgaaggcaa gacaacccag gagaacuuuu acagauucau caaaaaggcu     1140

auugagaaaa ucgaaggguc ugacuauuuu auugacaaga ucgaucggga agacuuccug     1200

agaaagcagc gcacauuuga uaauggaagc auuccccacc aaauccaucu gcaggagaug     1260

cacgccauca uucggagaca ggcugaguuc uacccuuuuc ucgucgaaaa ccaagacaaa     1320

aucgagaaga uucugaccuu ccgcaucccg uauuacguug gcccccuugc ccgggggaaa     1380

uccgaauuug ccuggcugaa uagaaagagc gaugagaaaa uucgcccuug gaacuucgac     1440

gagauggugg auaaggaaac cucugcugag aauuucauca cacggaugac gaacuaugac     1500

caguaccucc ccgaucagaa agugcugccu aagcacuccc ugcuuuauga aaaguuugcg     1560

gucuacaaug agcucaccaa agugaaguuc auugccgaag gaaugagaga cuaccaguuu     1620

cuggauagcg gccagaaaaa ggacaucguu aaaacacugu ucaagaccaa gcgcaaagug     1680

acagcuaagg auaucaaagc cuaucucgag aacucuaaug gguacgccgg agucgagcug     1740

aagggccuug aggaacaauu uaacgcuagc cugcccaccu aucaugaccu ccugaaaauu     1800

cugcgggaca aggccuucau cgaugcggaa gagaaucagg aaauucuuga ggacaucgug     1860

cucacgcuga cacuguuuga ggauagagaa augauucgca agcggcucga gaaauacaag     1920

gacauccuga ccgaggaaca gagaaagaaa cuugaacggc gccacuauac cggcuggggg     1980

agacuguccg cuaaacucau caacgggauu cuggauaagg uuacacggaa gaccauccug     2040

ggcuaccuua uugacgaugg gacaucuaac agaaauuuca ugcaacucau caacgacgau     2100

acgcugagcu uuguggacga gauucgccug gcccagggau ccggcgaggc cgaagauuau     2160

cgggcugagg uccagaaucu cgccgggagc ccggcuauca aaaagggcau ccugcagucu     2220

cuuaaaauug uggacgaacu gaucgagguc augggauacg auccugagca uauugugguu     2280

gaaauggcca gagagaacca auucaccaau cagggccgca gaaacuccca gcagcgguac     2340

aagaaaaucg aaaaugcgau uaagaaccuc gacagcaauc ugaacucuaa aauccugaag     2400

gaguauccca caaauaacca agcccuucag aaugaccgcc ucuuucugua cuaucugcag     2460

aacgggaagg auauguacac cgacgaggaa cucgauaucg accaacuguc ccaguaugau     2520

auugaccaca ucauuccuca ggcuuucauc aaagaugaca gccuugauaa uaaggugcug     2580

acaaaauccg ccaagaacag aggaaaaucu gacgaugucc ccagccucga gauugugcau     2640

aagaagaaaa acuucuggaa gcagcugcug gacucccaac uuaucucuca gcggaaauuu     2700

gacaaucuga ccaaggcuga acgcggcggg cucacgaacg aggauaaagc cagauucauu     2760

cagcggcagc ugguugaaac ccgccaaauc acaaagcacg uggcgagaau ccucgacacc     2820

cgguuuaaua caaagcugga ugaggccgga aaccgcauua gagaccccaa agucaauauc     2880

auuacccuua agagcaaccu ggugucccag uuccggaaag auuaccagcu cuauaaggug     2940

agagaaauca auaacuacca ccaugcucac gacgccuacc ugaaugcugu ugucgccacg     3000

gcccugcuua aaaaguaucc ucagcuggcu ccggaguuug uguacggcga uuaucccaag     3060

uacaacagcu auaaaucucg caaguccgcg acagagaaag uccucuucua cagcaauauu     3120

augaacuuuu uccggagagu gcugguguau ucuaagaccg gggaaguucg cauccggccu     3180

gugaucgagg ucaauaaaga aacaggcgag auuguguggg acaagaaguc cgauuuuaga     3240

accguucgca aagugcucag cuacccccaa gucaacgugg uuaagaaagu ggaaaugcag     3300

accggaggcu ucucuaagga gagcauccug cagcaugggg acuccgauaa acuuauuccu     3360

cggaagacag agaaauuuua ccuggacacg aagaaguaug gaggcuucga uagccccacc     3420

aucgccuacu cuguccuccu gauugcugac aucgaaaaag ggaaggccaa aaagcugaaa     3480

agagugaagg agcuuaucgg aauuacaauc auggaacgca uggccuucga gaagaauccg     3540

auugaguuuc uggaacacaa aggcuauaag aacauccucg agaaaaacau uaucaagcug     3600

ccuaaauacu cccucuucga acuggagaau gggcggagac ggcuucuggc uagcgccaag     3660

gaacugcaaa agggcaacga gaugauucug cccccucacc ucgugacccu ucuguaucau     3720

uccucuaaua uccacaaaau cacagagccc auucaucuca acuacgucaa uaagaacaaa     3780

cacgaauuua aggagcugcu ccgccauauc agcgacuucu ccaccagaua uauucuggcg     3840

caggaucggc uuucuaaaau cgaggaacug uacgacaaga augauggaga cgauauuagc     3900

gaccugacgu ccucuuuugu uaaccuccug acauucaccg cuaucggcgc cccugccgcu     3960

uuuaaguucc uugggagcgu gaucgaucgc aaaagauaca ccuccauugc cgaaauccug     4020

gaggcgacac ucauucacca gagcgugacc ggacuguaug agacacggau cgaccucucu     4080

aagcugggcg gggauuga                                                   4098


<210>  4250
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4250
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

aucacagaug acuauaaggu uccugcuaaa aagaugaaag ugcucggcaa uacgaacaag      120

aagaccauua aaaagaaucu gaucggggcc cuucuguucg augcgggaaa cacagcugcc      180

gacagacggc ucaaacgcac cgccagacgg cgcuacacaa gacggcgcaa ucggauucug      240

uaucuucagg agaucuuugc ugccgaaaug aacaagcugg augagucuuu cuuucaccgg      300

cucgacgaua gcuuccuggu gccggaagac aaaggcgggu ccaaguaccc caucuuugga      360

acccuggagg aagagaagga guaucauaaa caauucccua caauuuacca ccuccgcaag      420

ucucuggcug auagcaaaga aaaggccgac cuuagacuga ucuaucucgc gcuggcccau      480

auuaucaaau accggggcca cuuucuuuac gaggauaccu ucgacauuaa gaauaacgau      540

auccagaaaa uuuuuaauga guucacgucc aucuauaaca auaucuuuga agagucuagc      600

cuguccaacg aaaaugcuca ggucgaggaa auuuucaccg acaagaucag caagucugcc      660

aaaaaggacc gcgugcucaa acuguuuccc gaugagaagu ccacaggccu guucagcgag      720

uuccucaaac ugauuguugg gaacaaggcu gacuuuaaga aacauuucga ucuugaagag      780

auggccccuc ugcaguuuuc uaaggacacc uacgaugaag accucgaguc ccugcuugga      840

caaaucggcg augacuaugc ggaucuguuc guggucgcca aaaagcucua cgacgcuauu      900

cugcuggccg ggauccucag cgugaaagau cccgcuacaa aggccccucu gucugccucc      960

augaucgaaa gauaugacaa ucaccagaac gaccuuagcg cucugaagca guuuauucgg     1020

uccaaccucc cggagaaaua cgcggaaguu uucucugaug acagcaagga uggauaugcc     1080

ggcuacaucg acgggaaaac cacgcaggag ggauuuuaca aguauauuaa aaaucaaauc     1140

uccaaguucg agggcacaaa guacuuucug gaaaagauug agcgcgaaga uuuccuuaga     1200

aagcagcgga ccuuugacaa cggcagcauc ccccaccaga uccaucugca ggagaugaau     1260

gcuauucucc aaaaccaagg gggauauuac ccuuuccuga aagccaauaa ggaaagaauc     1320

gagcagauuc ugacauuucg cauccccuau uacgugggcc cucucgcccg ggggaacucu     1380

gauuucgcuu ggcugaccag aaauuccgac gaugccauuc gccccuggaa cuucgaggaa     1440

gucguggaca aagcgagcuc ugcugaggau uuuaucaaua agaugaccaa cuaugaccuu     1500

uaccugccgg aagagaaagu gcucccuaag cacagccugc uguacgagac auucgccguc     1560

uauaaugaac uuacgaaggu uaaauuuauu gccgaggcua ugaaggauua cgaguuccuc     1620

gacuccggac aaaaacggaa gaucguggaa aacaucuuua aaaaucauag aaaggugacc     1680

gaaaaggaca uuaucaacua ucugcacaau gucgauggcu acgacgggau ugagaucaaa     1740

ggaauugaga agcaguucaa cgccucucug agcacauauc acgaucuccu gaaaaucauc     1800

aaggacaaag aauuuaugga uaaccccaag aauggcgaga uucuugaauc caucauucau     1860

acccugacaa ucuucgagga ccgcgaaaug auuaagcagc uccuggcuca guacaacagc     1920

guguuugaug agaaaguuau caaggcccug ucucggaaac acuauaccgg cugggggaaa     1980

cuuuccgcga aacucaucaa uggaauuaga gacaagcaaa cgggcaacag cauccuggau     2040

uaccugaugg acgaugggaa gucuaaucgc aacuucaugc agcucauuaa agacgaugga     2100

cuguccuuua aggagaucau ugacaaagcc cagguggucg gcgaaacaga caaucuuaga     2160

caggugguuc aaaaccugcc ugggagcccc gcuaucaaga aaggcauccu ccaguccauu     2220

aagcuggugg augagcuggu caaagugaug ggaucugacc ccgaaagcau cgucauugag     2280

auggccagag aaaaucagac caccaacaag ggcaagucca aaucucagca acgccuuaag     2340

acacucagcg augcuaucuc cgagcugggg agcaauauuc ugaaagagca uccuaccgac     2400

aacauccagc uccagaauga ucggcuguuc cuuuacuauc ugcagaacgg aaaggacaug     2460

uacacaggcg aagcccucga uauuaaucaa cugucuaacu augacaucga ucacaucauu     2520

ccgcaggcgu uuaucaaaga cgauucccug gacaacagcg ugcuuacguc uuccaaggac     2580

aauagaggaa agagcgauaa cguucccucu cucgagauug uggagaaaau gaagggguuc     2640

uggcagcaac ugcuggacag caaacucauc uccgaacgga aguucaauaa ccugaccaaa     2700

gucgagcgcg gcgggcuuga ugaaagagac aaggugggcu uuauuaagcg gcagcugguu     2760

gagacaagac agaucaccaa acauguggcc cagauccucg augcucgcuu caaugccgaa     2820

gucaacgaga agaaucaaaa acugcggagc gugaagauua ucacacugaa aucuaaccuu     2880

guguccaauu uuagaaagga guucggacug uacaagguuc gcgaaauuaa cgacuaucac     2940

cacgcucaug augccuaccu caaugccguc aucgcuaaag cgauucugaa ggaguauccu     3000

aaacucgaac ccgaguuugu guacggcgac uaccagaagu augaucugaa aagauacauc     3060

agccggacca aggacccuaa ggaaaucgag aaagccacgg aaaaguauuu cuuuuacucc     3120

aaccuucuga auuucuuuaa agauaaggug uauuacgcug acgggaccau uauccagcgc     3180

ggaaacgucg aguauucuaa agauacaggc gagauugccu ggaauaagaa aagagacuuc     3240

gccaucgugc ggaagguucu cagcuacccc caggugaaca uugucaagaa aaccgaggaa     3300

caaacagggg gauuuuccaa ggaaucuauc cugccgaaag gcaacagcga caagcugauu     3360

ccucgcaaaa ccaagaauau ccagcuugau acgacaaagu acgggggcuu cgacuccccc     3420

gugaucgcuu auucuauucu gcucguugcc gauguggaga aaggaaagag caaaaagcug     3480

aaaaccguca agucccucau cggcauuaca aucauggaaa aggugaaauu cgaggcgaac     3540

ccuguugcuu uucuggaggg gaagggauac cagaaugugg ucgaagagaa cauuaucaga     3600

cuucccaaau auagccuguu cgaacucgag aauggccggc gcagaaugcu ggccucuucc     3660

aaggagcugc agaaagggaa cgaaauggug cuuccuagcu accugcucgc ccugcucuau     3720

cacgcuaaga agauccagaa aaaggacgag cccgaacauc uggaguacau uaaacagcac     3780

cacucugaau uuaaugaucu ucugaacuuc guguccgacu uuagccagaa guauguccug     3840

gccgagucua aucucgagaa aaucaagaac cuguacauug auaaugaaca aaccaaccuu     3900

gaggaaaucg cuaauuccuu cauuaaccug cucaccuuua cagcguucgg agccccggcc     3960

guuuuuaaau ucuuuggcaa ggacaucgag cggaagcgcu acagcacggu gaccgaaauc     4020

cugaaagcua cacucauuca ucaguccauc accgggcugu augagacaag aauugaucuu     4080

agcaagcugg gcgaggaaug a                                               4101


<210>  4251
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4251
auggacaaga aauacaacau cggccuggau auugggacca gcuccaucgg auucguggcc       60

acagacgagg auaaucacgu cauuagagcu aagggcaaaa acguuaucgg ggugcggcuc      120

uuugacgaag gacagucugc cgcggagcgc agaggcuucc ggacgacccg cagacggcug      180

aaucgcagaa aguggcggcu uaaccugcuc cgcgaaauuu uugagcccca uaucuauccu      240

aucgaugcua gcuucuuuau gagacaaaaa uacuccaauc ugucuaagca ggacccgaac      300

cagaagauug aguucaaaga uauccuuuuu agcgacaagu ccgaugccga guucuaucgg      360

caguacccca caauuuauca ucugcgccaa gcccucauga ccgagaaaag acaguuugac      420

cugcgggaaa ucuaccuggc uauucaccac aucgugaagu aucgcgggca uuuccucgau      480

gagacaccua ccgacaaauu uucuuucgau cagcuggacc uuacacagga auuugagaga      540

cugaaugucc ucuucagcga gcaaaacccc gaagaaccuu ucgaaauugu gguuaaucag      600

aaccagucca ucgaggacau ccugagcaau cagucuauga ccgccuccga ucggagaaag      660

caagcucuug uggucuuuua ccagcagagc gaaaacaagg agaaugagaa acaauacaag      720

gccauugcga cggaaauccu gaaccuccug cugggaaaua aagccaagcu caacgugauu      780

cuggcucagg agguugacaa agaagccgcu aaggauuggu cucuuucccu gagcucugag      840

accuucgacg augaccucga aaaagccucc agcggccuga acgauuccca gcuugagaug      900

gugggcaucu uugagcgcau uuauucuagc cugauccuca aguccaucau uggggaccac      960

aauagccugu cugcggccau ggucgauaag uucaaaacac acgcucagca ucgggaccug     1020

cucaaggaug ugaucaaaaa cuccgacgcc accaagggag aacaacugca guuugcuuac     1080

gaccacuauc uucagagaca uagcgccgau ggcaaagccu acgggaagga cgauuucuau     1140

aaggagguga aaaagaaucu ggacgauucu gacgcugcgc agcaaauuca gcagcucauc     1200

gauacagacc aguuuaugcc caaacaacgc accaaggcca acggagcuau uccucaccag     1260

guccagcaga aagaacugga ucuuaucauc gagaaucaau gccaguacua uccguggcug     1320

gccgaagaga accccaauag cgagcggcac aacguugcca aguacaagcu cgacgaacug     1380

guguccuuca gagugccuua cuaugucggc ccccugauuu cuccugcuga ugacgagacg     1440

gauccccagg ccauccaaaa uacaaaauuu gcguggauga uucgcaagga cgcucagggg     1500

cggaucaccc cguggaacuu cgacgauaaa guggacagag aaaagagcgc cgauaaauuu     1560

auuaagcgca ugacaaccac cgacacauac cucaucggag aggauguucu gccugaccag     1620

ucccuuauuu aucagcggua caaggugcug aaugagcuca acaaugucag aaucaacggc     1680

cgccccaucg aagugcaaau uaaacagaau aucuauacgg aucuguucaa gaaauuuaag     1740

agcguuucug ugaaaaagcu gcgguccuac cuugaggaaa ugagcuauua cccuggcucu     1800

uucaaaauuu ccgggcucac caaccccgga aaguucgaca acggccugac aagcuaccug     1860

gauuauaaga auauccucgg ggacuccguc gaugccaacg acaaaagagc ugauauugag     1920

cagaugaucg aauggucuac caucuuugag gacaauaaga uuuucgccca gaaacuggag     1980

agcaugacau ggcuuaccgc ugaacaacgg aaggccaucu cccgcaaaag auacacggga     2040

uggggccggc ugucuaacaa gcuccugaca cagcugaaga aucaggacaa cagaagcauu     2100

auggaucuuc ucugggagac cgaagagaau uuuaugaaaa uccaguccgc gcccgacuuc     2160

agcaagauga uugaagccca uaaccaacag uuuaucaaaa augaggcuaa cauccagcaa     2220

gugauuucug aucuguauac cuccccucag aauaagaaag ccauccgcca gauucugcuc     2280

gucguggacg auauccagaa ggcuguuggg cacgccccgg agcauauugc gaucgaguuc     2340

gccaagggag aggacaaaaa cccccggaga acaaucagcc gcgaaaugaa gauucaagau     2400

guguaccagc agaucucuga cgauauucug gucuccccug aggugagagc ucagcuuaau     2460

gagaaaaucg aaaaccggca cggccgccug agcgacagac ucuuucugua uuucaugcaa     2520

ggcgggcggg auauguacac cggaaagccc auuaauauca acaacauuuc ucauuaugac     2580

aucgaccaca uccugccuca ggccuuuauu aaagaugaca gccuugauaa ucgcguucuc     2640

guguccgaca agcugaacag agaaaagagc gauacagucc cccgggaccu guucggccgc     2700

gaaaugaugu cuacguggga gaaaauggcu gauagagggc ucaucaccaa gcggaaacug     2760

gccaaucuua agacaauucc gggauccauc gacgcccaca aagcucaggg cuuuauuaac     2820

cgccagcugg uggaaaccag acaagugauc aagcucgcgg uugggaucau gauugaucag     2880

uacccugaga cacagaucgu cagcgugaaa gccaaucuga cccaugagau gcgggaaaug     2940

uucgaccugg ugaagaacag agaugucaau gacuaccacc augcuuuuga ugccuaucuu     3000

acggccuucg ugggcaacua ccuccugcag cgcuauccca agcuggagcc uuacuucguu     3060

uaugacaaau uuaagaaacu caaggaccaa aauauuucca ugcgggauuu caaccugcuu     3120

uacgaccuga aaaagaauga agauguguau gaccagagaa ccggacacuc ugcucacaag     3180

aucugggauc agcauggcag cauugacuac auccgccacc ucuuugccua caaaaagauc     3240

cuggucuccc augaggugca ccaaaacggg ggagcgcugu auaauaugac aguuuacggc     3300

aaagcugaug acgccaagcg gaaacuuauu cccaucaagg ccgauaagcc uaccgaacug     3360

uaugggggau acacaucuag aaaacucgcu uauauggcca uugugaagac caaaaagggc     3420

aaaaaggagc aguaccaggu cacgggggug cccguuagcg cgcugacaac ccuccgccag     3480

uucaaggagg acuccccgga acaaaaacag gcucuggagg aaaucgugcg gagaaacuuu     3540

gaugagaaga cagaagucga gauuaucgug ccuaaagugc uuuucaauca gcugauuguc     3600

gacgagaagu gucgcuuuau gguuggcucu gauaccuauc gguacaacgc ccagcaacuc     3660

gugcugagcg ccgacgaccu ggaaaucauc aacgcuuccc auaaagauau ucagaccauc     3720

gacagcucug auuacuccca gcagcuugac caaguguauc agaauauucu gacaaucguc     3780

gauaagcacu uccccaugua cgacauuaac aaguuuagaa cgaaacucca cgauaucuau     3840

gagcaguaca ucgaacugcc caaugaggac gugugggaaa acaauaagcu caccacacug     3900

ggaaaacaua ccauuaucau ucggauccuu caaggccugc acgccaacgc uacaguugug     3960

gaucucaagc agcuggggau uaaaaccccu uucggauuua ugcagcaggc ccgcggcauc     4020

gucgugagcc cggacgcgca acugaucuau cagucuccca cggggcuuua cgagagacgg     4080

guucgccugg gagaucucug a                                               4101


<210>  4252
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4252
augggcgaca gaaaguacaa ccuggggcuc gauaucggaa ccagcuccau uggcuucgcc       60

gcuguggacg agaauaacca gcccauccgg gucaaaggga agacagccau uggaguucgc      120

cuguuugaag agggcaaaac ggcggcugau agacgggggu uccgcaccac aagacggcgc      180

cuuucuagac ggcgcuggag aaucaaucug cucaacggaa uuuuugacgc ccaccuggcc      240

gaaguggauc cuaccuuccu ugcucggcug aaggagagca aucgcuccaa ccucgacccg      300

aaaaagucuu uucaaggcag ccugcuguuc cccgaaagaa aggauuauca guuuuacaaa      360

gaguauccua caaucuacca ucuccggaag gcccugaugg aggaagaccg caaauucgau      420

aucagagaga uuuaucuugc ugugcaccau aucauuaagu accggggcaa uuuucugaac      480

gggaccccca ugagauccuu caaagucgaa aauaucgagc ucgacacacu guuugaucgc      540

cuuaaccagc uguaugagga aauuaucccu gacaaugagc ucgccuucga ucuggaacag      600

guggcggacg uuaaggaggu gcugucuagc accacgauuu acaagaugga caaaaagaaa      660

caacucguca agaugaugcu gcuucccacc uccaacaaag cccugcagag cgaaaauaag      720

aaaaucguga cacaguuugu uaacgcuauc cucaauuaca aguucaagcu ggaugugcuu      780

cugcaggucg agaccgacgc cgauuggucu cucaaacuga acgacgaggg agcugaugac      840

aagcuggaag aguucacagg cgaucucgac gaaaaccggc uggagauuau cgaucuucug      900

caaagacucc acaauugguu uucccugaac gaaauuacca aagacgggaa uagccuuucu      960

gccgcgaugg uggagaagua uaaagaucau cgccaccacc uggagcuccu gaagaaggug     1020

aucgaagacc auccugacgc caaaaaggcu aaagcccuga aggagacgua cacagcuuau     1080

gucggaaaaa ccgaugacaa gacacagaac caggaugacu ucuacaaggc cguugaaaag     1140

aaucucgaug acuccccgga ugccaaggag auuaaacggc ugauccagcu ugaccaauuu     1200

augcccaagc agagaaccgg ccagaacggg gcuaucccuc accaacugca ucagcaggaa     1260

cucgaucaga uuaucgagaa acaaagcaag uauuaccccu uccuggcgga gccuaauccc     1320

aacgugaagc gccggaaaga cgccccguau aagcuggaug aacuuauugc uuuuaaaauc     1380

ccuuacuacg ugggaccccu cgucaccccu gaggaacagg cccagaaugg cgagaacgug     1440

uucgccugga ugaagagaaa aguugcuggc cccauuacac ccuggaauuu ugacaagaaa     1500

guggaucgca uggagucugc caaccgguuc aucagacgca ugacgaccaa ggacacauau     1560

cuguuugggg aagacguccu gccugcggag uccaugaucu acgaaaaguu cgugguucuc     1620

aaugagcuga acaaucuuaa aauuaacgga cggcaccuga gccucaagga uaaacaggac     1680

aucuauaacg aucuguuuaa gcaacagaaa accgugucca uuaaggcucu gcagaauuac     1740

uaugucacaa agaaaaaggc cgccaccgcu ccgacggugg gcgggcuugc cgaccccaaa     1800

aaguuccucu cuagccuguc cacauacauc gauuuuaaaa acauguucgg agaacagguc     1860

aaugacccuc aauuccagga ggaucuggag cagauugugg aauggagcac caucuuugag     1920

gacagaggca uuuucaaggc uaagcuccag gcccuggggu ggcuuucuga aaaacaaauc     1980

cagcagcugg uugcgaagcg guauaaagga uggggccgcc ucuccaagaa acugcugacc     2040

ggccuuaaga acgccgaggg guacagcauc cucgaugaaa uguggagauc uacagagaau     2100

uuuaugcaaa uucagagccg gcccgaguuc gcugcccuga uccagcaggc uaacgaaaag     2160

caauuugagg gaaaugaccc uaacaaugug ugggaaaaca uugagaauau ccugggcgau     2220

gccuacaccu cuccccagaa caaaaaggcg auuagacagg ucgugaaagu ugugcaggac     2280

aucgaaaagg ccgucgggaa uccgccugag aaaaucgcua uugaguucac acgcgaagcc     2340

gcugccaacc cccaacggac gcaguccaga cuccgcaccc uggagaaacu uuaugaaagc     2400

gccgaggagg ugguggaugc uagacugaca gcggaacucg ccgaguuuaa agaaaauaag     2460

cacguucugu ccgacaagua cuaucuguac uucacccagc uuggacggga cgucuauaca     2520

ggcgauacca ucagccucga ccgccugaac gauuacgacg uggaucauau ucugccucag     2580

ucuuuuauca aagacgauuc ccucgacaac agagugcuga cgauucgggc ugucaauaac     2640

ggcaagagcg auaaugugcc cgccaaaaug uucggcaaga aaaugggauc uuuuuggcgc     2700

uaucuucugg acaacggcau gaucuccaag agaaaguaca auaaccucau caccgauccu     2760

gacaauauua gcaaauacgc ccaaaagggg uucaucaacc ggcagcuggu ugagacaucc     2820

caggugauua aacugaccgc uaauauccuu aacggaauuu augacaagga uacagaaauc     2880

aucgaggucc ccgccaaaau gaacucucaa augcgcaaga uguuugaccu ggugaagguu     2940

agagagguga augauuacca ccaugcguuc gacgcuuauc ucaccauuuu caucggcaau     3000

uaccuguaua aaugcuaccc gaagcuccgg ccuuauuuug ucuacgggaa auucaagaaa     3060

uuuagcgaua acgaagacau ugagguggga gauggccgcu ucaauuuucu gaacagaauc     3120

gaacggcuua agaacguuac ggaccccgag acaggggaaa uucugugguc caauguggcc     3180

cccaacgaga ccaucaagag aauuaaaaag gucuacaauu auaaauucau gaucgugucu     3240

cgcgagauca caacccggag agccgaacuc uuuaaccaga ccguguaccc uaagaauuau     3300

cgcggcaaac ugauuccgau caaggaggau cggcccacag accuguacgg aggcuauagc     3360

gggaacacgg augcuuaccu ugccauuguc gcgcuggaag acaagaaaaa gggaaaauau     3420

uucaagguug ugggcauccc uaccagagcu gccgccaaac ucgagaagcu gaaacagcag     3480

gauucccaac aguaccucca ggcugugcac gaagucauug cuccccaguu uacaaagagc     3540

accaagaaag ggaucaagaa aacagaguuc cgcaucgugc uggacaaggu ucauuaccgg     3600

caacuugugc aggacggacc ugucaaaaug augcuggggu cuuccaccua uaaguacaau     3660

gccaagcagc ucgugcugag cgagaaagcc cugcagguua uuaaggauga cagaaaauuu     3720

gaugaaacgc aaaaggacga uaaccuuauc gcgguguaug acgagauucu gucuaucguc     3780

aaucaguccu ucgaucucua cgacauuaac ggguuccgca aaaagcugaa ugauaaccgg     3840

aaucaguuua ucgaccuccc cgccgaaaca aaguaugagg gcagaaaagu gguugcucac     3900

ggaaagcggg agaugauccu ggaaauucuu aaaggccugc acgccaacgc uagcuucggg     3960

aaucugaagc cuaucggauu ugccaccccc uucggccaga ugcaaucuaa aaacgggauu     4020

gagcucagcg aaaaugccuu ucugauccau caguccccga gcggacuuuu cgagcacaag     4080

auuaagcugu cugaucucug a                                               4101


<210>  4253
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4253
augaccaagc ugggcaaacc cuacgggauc ggaguggaca uuggcagcaa cuccaucggg       60

uucgccgcug ucgaugaaaa uucucaccuc auuagacuga agggaaaaac aguuaucggc      120

gcccggcuuu uugaagaggg gaaggcggcu gccuaucgca gagccagccg gacgacccgc      180

agacggcugu cccgcaacag auggcggcuc ucuuuccugc gcgacuuuuu cgaaagccau      240

auuacaccua ccgauccgaa cuuuuucaug agacagaaau acuccgagau cucucccaag      300

gacaaggcuc gguauaaaua cgaaaagcgc cuuuuuaaug auagaacaga cgccgaguuc      360

uaucaacagu acccuaccau guaucaucug cggacacgcc uccugaccga ucccagcaaa      420

gcugacguga gagagaucua cuuugccauu caccacaucc ugaagucccg gggacauuuc      480

cucacgccug gcuacgcgaa agauuuuaau accaacaagg uggcccugaa ugaaauuuuc      540

cccgcccuuc aggacgcuua ugcucagguc uacccugauc uggacaucac auuugaugag      600

aacaagguga augaguucaa aaccguucuc cugaacgaga aggccacacc gagcgacacc      660

caaagagcgc uugugaaucu gcuccuggcc aaagagggcg accgcgauau ucugaagcag      720

cagaaacagg uccucacgga guucgcuaag gccgugguug ggcugaaaac aaaacuuaac      780

guggcucugg gaaccgaagu cgacucuucc gaagccacag ccuggaauuu uagccucggc      840

caacuggaug acaagugggc ugggaucgag ucugcgauga ccgaugaagg aaccgagauu      900

cuugaccaga uccgggaucu guauagagcc cgccuccuga acggcaucgu gcccgcuggg      960

aaaacacugu cccaggccaa gguggacgau uacgcccagc accacgagga ccucgaacug     1020

uucaaagauu aucuuaagca acuggaggac gacggaacgg cuaaagccau ucggcagcuc     1080

uacgauagau auaucgacgg cgaugacgcg aagccuuuug uccgcgaaga uuucguuaag     1140

gcucugacca aagaggugac agcccauccc aaugccaaga gcccugaacu ucuggagcgg     1200

cucgcucagc ccgacuuuau gcugaaacaa agaaacaagg ccaacggcgc uauuccggug     1260

cagaugcagc agcgcgagcu ggaucaaauc auuaaaaauc aggccgucua cuacgacugg     1320

cucgcggccc cuaaccccgu ggaaaagcac cggcagucua ugaccuauca gcuggaugag     1380

cuucugaauu ucagaauccc uuacuauguu gggccccucg ugacagcuaa ggaacaaaaa     1440

gccgcucagg gaggcgucuu ugccuggaug gugcgcaagg accccgaugg gaacaucacc     1500

ccuuacaauu ucgacgagaa aguugaucgg gaagcguccg ccaacacguu uauucagaga     1560

augaagacaa ccgacaccua ucugaucgga gaggacgugc ugccgaaaca gagccuucuc     1620

uaccaauccu augagguccu gaaugaacug aacaaugugc gggucaacga uaauaagcuc     1680

ucuuucgacc agaagcaaca ggugcugggc gagcuuuuug aacgccauaa cacaguuacc     1740

auuaaacagu ucguggauaa ucugcucgcu cacggggacu acgauagaau ccccgagguc     1800

cggggacugg ccgacgagaa gagauuucug agcucccuua gcacauacaa ccagcucaaa     1860

ggcauucugc acgaagcuau cgaugacgcc gauaagcaga cggacaucga gaauauuauc     1920

gccuggucua ccauuuucga agauaaagcu aucuucaaga caaaacugaa cgagauuucc     1980

uggcucaacg cgggcaugau caauagccug gccaacauuc gcuaucgggg guggggacaa     2040

uuuucucaua aacuucugaa ugaacucacc cugagcacag gcagaaccgu guucgaggag     2100

cugcuuaucu ccucucgcaa ccucaugcag auccugacgg acgaaguucu gaagcagaag     2160

auugcugagc ucaaugccca cgaacugaag gccagcggga ucuacgcugc cauugaugac     2220

gcguauaccu ccccuagcaa caaaaaggcu cuuagacagg ugcugucugu caucgacgau     2280

auuacaaaug ccgccggaca agcucccaac uggcucuaca ucgagaccgc cgacggcgau     2340

ggggugccug gcaaacggac aaauucccgc cagagaaaga uccaggaaau uuaugcgaac     2400

gcugcccagg agcugaucac cggaagcgug cggggcgagu uugaagacaa gauugccucu     2460

cgcgcugauu ucacggacag acugguucuu uacuuuaugc aagggggacg ggauaucuau     2520

acaggcgagc cccucaauau ugacaaccug uccagcuacg auaucgacca uauccugccg     2580

cagucccuca uuaaagauaa uucucuggac aaccgcgucc uugugcaccc ucuggugaau     2640

agagaaaagg acaccaucuu cgccagcaca cgguuugcua aacagaugaa cgggaugugg     2700

cgccaguggc augccgcggg acucauuucc ucuagaaagc ugcggaaccu gcaaaugaga     2760

cccgaugaga ucgacaaaua cgccagcggc uucgucgcuc gccagcuugu ggaaacccgg     2820

cagauuauca agcugaccga gcaaaucguu gccgaucagu auccugacac aaagauuauc     2880

gcugugaaag ccgggcucuc cagccagcug agaaaggagc ucgauuuucc caaaaaucgc     2940

gaagucaacc acuaccacca ugccuucgac gcuuuucugg cggcccggau uggcacguau     3000

cuucugaaga gauacccuaa ucucgagccc uucuuuaccu auggaaaauu caagaagaca     3060

gaagugaaaa agcugaaauc ucugaagucc cuuaagagcu ucaacuuuau ccgcgauaug     3120

acccacgcua aggacaaaau uguugccaag gagacaggcg agaucgugug ggauaaugcc     3180

ucugacauua acgaacugga ucggaucuac aauuucaaga gaaugcucau cacccaugag     3240

guccgcuuug aaacggcuuc ccuguucaaa cagacacucu augccgcgaa gaacagcaaa     3300

aauagagggg gaucucggca acugauuccg aagaaaaagg gcuaccuugu ggacaucuac     3360

gggggauaua cccaggagac cggcagcuac cuguccguug ugcggcucac aaagaaagcu     3420

auguaugccg ucgugaaggu gagcacccgc gaugccgcua aacuggccgu cgcgaagucu     3480

auuuccgaac agaaagagaa cgagacacug aagaagauca uugacgggaa acuuagcaag     3540

acguccaaaa agggcaaaac cacacaccag cuguuugaaa ucguucuccc uagaguggga     3600

caaaagaccc uguucaaaaa uucuaaguac aaccaguuuc ucgugaauag cgacacauau     3660

augcacaacu accaggagcu guggaugccc cgggaauauc agcgcaugug gaaggauauc     3720

cuucugucca aucauggcga cgcucaaauu gaggggcagc uggaucagau cuucaaauuu     3780

auugucucuc aggugaacag cuacuucaac cucuacgaca ucaaucaauu uagaaagaaa     3840

auugccgaug ccaccgacaa guucgcuaag cugcccacgc acgauaccga caacgcccgg     3900

ggaaaaaucg cuacaaucgg ccagcuucug auugggcucc aggccaaugc gaugagaucc     3960

gaucugcgca accucgacau caagaccccu uucggacugc uucaaauugg cacagggauc     4020

acccuggauc uggacacauc uauuaucuau cagagcccga ccggccucuu ugaacgggag     4080

guuccccuga cggaucuuug a                                               4101


<210>  4254
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4254
augaccaagg acuacagccu gggccucgau aucgggacag gauccguggg cuucgccgcu       60

cuggacacga acuauagacu ucugcgggcc aaagggaaua acgucauugg aguucgccuc      120

uuuacccccg cggagacagc ugaagagaga cggggcuucc gcaccgccag acggcgccug      180

ucuagacggc gcuggagacu uggggcccug aauggaaucu uugcuccuga acucgccaag      240

guggauccga acuuccugca ccggcugaaa uacgcuuggg ugcaucccgc cgaccagcaa      300

aauaagggca aauauagcgc ggccccucuc uuuaacgaug cugcccugga ccgccaguuc      360

cacgaugacu accccacaau uuaucaucuu agacugaagc ucaugaccga uacaucccag      420

cacgaccugc gggaggucua ccuugcuauc caucacauua ucaaguuucg cggcaauuuc      480

cugagagggg aaggaccuau caacgccucu gaggcguuug auauuaauac cuucaucgac      540

cagaugacgg ccuuuuauga ucaggcuuuc cccgacuuua aaauugugga ccaaguugau      600

cagcagauca uuuucaaggg ccucaccgac augacacuga gcccugccga ucgggcuaac      660

aaagcccuga ugggguucaa uaacgccgcu caacagccga auaagagaau cuuuaaagcg      720

auucucuccg gacugguggg caaccagguc gaccuuaugg ccaucuucca gcuggauggg      780

auccccaagg aggaccaaaa ggaauuuaaa uucaaguuug uggauaauga cauugaggaa      840

aaacucgcug cccugggaga uaacccugcc cuuucugacg agcagaacga guuccugacc      900

acacucuacg cugccuuuag cggccugacc cugaagggcc uccuggggga ugcgacgucc      960

aucagcgcug ccaugguugc ccgcuacgcu ucucaccgga cagacuggac cuauauuaaa     1020

gaccaugugc uuacacccca gaauaacaga gaacugcgcc ucgccuacgc ugaucugaug     1080

accgacaaug aggauguccg gcuuaaggcc ggaaagaagu ucgcgaaggc caucgaagcu     1140

gccgacauuc cugcugccaa aaaguccgag uuuagccuga aaaucgauaa caaugaguuc     1200

cuccccagac aacgcaccaa ggcgaacggc acaaucccgc accagcugca ucugaaugag     1260

cuccagcaga uuaucgagaa ccaagggcag uauuacccuu uucugaaagc cacguucgaa     1320

caggacggac acacccuuaa uaagauugag ggccugguga aguuucgggu gcccuauuac     1380

gucgggccuc ucguucccaa acacaaggug gcuggaaaca augccaacca uuggauggug     1440

cccaaagaac cuggcgcuau cacaccgugg aauuauaccg agaaggucga uagagacaaa     1500

ucuggcaacg aguucauucg ccggaugaca gggaccgaua cguaccugcu gggagaaccc     1560

acacuuccua aaaauucccu cguguaccag gaguucaacg uucugcaaga acugaacaau     1620

cucagaaucg agaagcgccg gcuggacauc gccgugaaac aggauauuuu ugaacacguc     1680

uucaagacca acaaaaccgu gacaguuaag agaaucgagg aauaucuuac cacagaguuu     1740

ggcagccccg ugacgcuguc uggguucgcc aaugucaacg agcggaaacu caauuccagc     1800

cuguccaccu accauuauuu ugugggaauu uucggcgcug aauuugucga cgauccuaag     1860

aaccugacac uucucgagca gaucguggaa cugcagaccg uuuucgagga ccgcaaggcg     1920

cugcaaagac agcucaaacu gcggcccgag cuuucugaaa aacaggugaa gcagcuggcc     1980

agaacacacu acaccgggug gggccgccuc agccaaaagc ugcugacguc caaaauuguc     2040

cauguggcuc uugagggaga acgggagccg ggccaguaua gcauccucga aacccuguac     2100

gggacacaga ugaauuuuau ggagauuguu aacgacauga aggauagaua uggagugaaa     2160

ucuuggcugg accaagaaaa uuccggcagc gaaaacaauc ucucucugaa gcagcgcauc     2220

cuugauaugc cugggcccaa gaacaucaaa agaggaauua aucaggccuu ccacaucauu     2280

gacgagauca cccgggccgu cggcaacgcu ccuagccgca uuuacaucga auuugccaga     2340

gagucccagc aaucuaaucu gacaaacgcg cgggugaacc gccucaagca gcuguacagc     2400

aaucaggcuc ugaaaaagga guucgccaaa cuugccuccg aaaucaagga ucucaccaag     2460

gagcagcuga aagacgauag acuguaucuc uacuaucugc aacaggggaa ggacauguac     2520

acggaaacag auauugacau caccaaaauu agcucuaacu aucagaucga ucacauuauc     2580

ccccaggcuu acacaaagaa uaacucccuu gacaaucggg ugcugguuaa cagcgagaug     2640

aaugcccgca aaucugauuc cccuaccuuu cuccccagcc ugauccaaga aagaaaggcg     2700

ugguggcggc agcugcgcga caccggccuu auguccacaa uuaaguauaa caaucugacg     2760

agaaccucug acgauuucag cgcugaccag aaagagcggu ucaucgccag aucccucguc     2820

gaaacacgcc aagugauuaa gaacguggcc acccugauca aagaguacua cgaugacucu     2880

caggucgagg cucucccgag cgaaauuaca gccgagaugc ggagauaucu gaaugugcgc     2940

aagaaccggg auaucaauga cuaccaucac gcucaugaug cccuucuguu ugcgaccgcc     3000

ggagaguucu ccagaaaagu uggcaucaug gaaaaggggc gcgugagcga cggagcuggc     3060

aacauuuaua aucgguacac gaaggauauc auucagagag cccgcgcuaa agccgggcag     3120

gacaagaaag uccggccuuu uacauucauc gugggaucua uggccaaccc cgauaccaag     3180

uuucaaacca accagacaac cggcgagauu guuuggacac ccgaagacgu ggcuuaucuc     3240

agaaaaguca ucgacuacaa gaaaaugcug gugacgaaga ugacccugga ucagacaggg     3300

acccuuuaua augagacacg guacggcucc ggagacaagc acguugcggu ggcccugagc     3360

aaagauaagg cugccgaccu cuacggcggg uucggaucuc ugaaaccugc cuaugcugcc     3420

cucgucuacu ccaagaaaaa guuucgccug gugaacgugc uuagacugug gcucccggaa     3480

auuaagaaaa augcgagcgu ccuggagcgg aagguucagu cugugcuggg cgaugacaac     3540

gcuaaaguga uccgcgccca cguccccugc gggcaacuuc ugaucaagga uggagcccuc     3600

gugaccguua gcuccgcuac ggaacugcau aauuucagac agcucuggcu gccuaaagcc     3660

gacuaucagg auauuagcau ccuucugggc gcgcccgaca aggugaccgc ugagaaccgg     3720

cugcaggccg agucucacuu ugaugacguc gaucaaagau ucgaaucccu ccugaaggac     3780

gugauugacc aggcccgcca guacuauccu cuuaaaacac aucagaccag ccuggagaag     3840

cucaucggga aaauuggcaa guuugaugaa cugaaauucg cucaaaagca gcagguuuuc     3900

ucccggcucc ugucugcccu ucacgcuaau gccacaaaca gcgaccugaa gcaaaucgga     3960

cuguccaccg cguggggcag acucaucuac cccaaagggg ugucucugac agaugaggac     4020

aaacuuauua auagcuccuu uaccggacug uucaacgaug ucaugucuau caaggaauuu     4080

caugacuuca gcauuaauug a                                               4101


<210>  4255
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4255
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugca uuacagaccu cgauuacaau guccugagau ucaacaaaaa ggacaugugg      120

ggcucucggc uuuuugagga agccaaaacg gcugccgagc gcagaguuca acggaauagc      180

cgcagacggc ugaagcgcag aaaguggcgg cucaaccugc uugaagagau cuucuccaau      240

gaaauucuga aaaucgauuc uaacuuuuuc cgcagacuca aggagagcuc ccuguggcug      300

gaggacaaau cuagcaagga aaaauuuacc cucuucaaug augacaacua uaaggauuac      360

gacuuuuaua agcaguaccc cacaauuuuc caucugcgga augagcuuau caaaaacccu      420

gaaaagaaag auauccgccu gguguaucuc gcgauucacu ccaucuuuaa gagcagaggg      480

cacuuccugu uugagggaca gaaucuuaaa gagauuaaga acuucgaaac ccuguacaau      540

aaccucaucg cuuuccugga ggacaauggc auuaacaaaa ucauugauaa gaacaauauc      600

gaaaagcugg agaaaaucgu gugugacucu aagaaagggc ucaaggacaa agaaaaggag      660

uuuaaggaga uuuucaacuc cgauaaacag cuggucgcca ucuuuaagcu gagcguggga      720

ucuuccguua gccugaauga ccucuucgau acagacgaau acaaaaaggg cgagguggaa      780

aaagagaaga uuucuuuucg ggaacaaauc uaugaggaug acaagccgau uuacuauucc      840

auccugggcg agaaaaucga acuucuggau auugccaaga gcuucuacga cuuuaugguc      900

cucaacaaua uccuggcuga uucccaguau auuucugagg ccaaagugaa gcuguacgag      960

gagcauaaaa aggaccucaa gaaccugaaa uauaucauuc gcaaguacaa uaaagggaac     1020

uacgauaaac uuuucaaaga caagaaugaa aacaauuaua gcgcuuacau cggacugaac     1080

aaagagaagg gcaagaaaga gguuguggaa aaguccagac ucaaaaucga cgaucugauu     1140

aaggucauca aaggguaucu ucccaagccu gagcggauug aagagaagga caaaaccgug     1200

uuuaaugaga uccugagcaa gauugaacuc aaaacaaucc ugcccaagca gagaauuucu     1260

gauaacggaa cccugccuua ccagauccac gagguggaac ucgagaaaau ccuggaaaau     1320

caauccaagu auuacgacuu ccuuaacuau gaggagaacg gcgucagcac gaaggauaaa     1380

cugcucaaga ccuuuaaauu ccgcauuccc uacuacguug ggccucuuaa uucuuaucau     1440

aaggacaaag gaggcaacag cuggaucgug cggaaggaag agggcaagau ucugccgugg     1500

aauuuugaac agaaagugga uaucgagaag uccgccgaag aguucauuaa aagaaugaca     1560

aacaagugca ccuaccuuaa uggggaggac gucaucccca aagauacauu ccucuauucu     1620

gaauacguga uccugaacga gcugaauaag guucagguga acgacgaauu ucucaaugag     1680

gaaaacaaac gcaagauuau cgaugagcug uucaaggaga auaaaaaggu cagcgaaaag     1740

aaguuuaaag aguaucuucu ggugaagcag auuguugacg gaaccaucga acucaagggc     1800

gugaaagauu ccuucaacag caauuacauu ucuuauaucc gguuuaagga caucuucggg     1860

gagaaacuga accuggacau uuacaaggag aucuccgaaa agagcauucu uuggaagugu     1920

cucuacggag augacaagaa aaucuuugag aagaaaauua agaaugaaua uggcgauauc     1980

cugacgaaag acgagaucaa gaagauuaac acauucaaau uuaacaauug ggggagacug     2040

ucugaaaagc uccugaccgg aaucgaguuc auuaaccuug agacaggcga augcuacucc     2100

agcgucaugg augcgcugcg ccggaccaau uauaaccuca uggagcugcu guccucuaaa     2160

uuuacccuuc aagaaagcau caauaacgag aauaaggaaa ugaacgaggc cuccuacaga     2220

gaccucauug aggaaucuua ugugagcccu ucccugaaac gcgcuaucuu ccagacacug     2280

aagauuuacg aggaaauccg gaagaucacg ggcagagucc ccaaaaaggu guuuauugag     2340

auggcccgcg ggggagauga aagcaugaaa aauaagaaaa ucccugcucg gcaggagcaa     2400

cucaagaaac uguaugacuc uuguggcaac gauauugcca auuucuccau cgacauuaag     2460

gagaugaaga acagccuuau cucuuacgau aauaacuccc ugagacagaa aaagcucuac     2520

cuguauuacc ugcaguucgg gaaaugcaug uauaccggac gggaaaucga ccuugaucgc     2580

cuccugcaga auaacgacac auacgacauu gaucacaucu aucccagaag caagguuauu     2640

aaagacgauu cuuuugacaa ccuggugcuc guccugaaga augagaacgc ggaaaagagc     2700

aaugaguacc cggugaaaaa ggagauccaa gagaaaauga aguccuucug gcgguuucuu     2760

aaagagaaga acuucauuag cgaugaaaag uauaaaagac ugaccggcaa ggacgauuuu     2820

gagcuccgcg gguucauggc ccggcagcug guuaauguga gacagacaac caaagaaguc     2880

ggcaagaucc ugcagcaaau cgagccugag auuaaaaucg uguacucuaa ggcugaaauu     2940

gccuccagcu uucgcgagau guucgacuuu aucaagguga gagaacuuaa cgauacgcac     3000

caugcuaaag acgccuaccu gaauauuguu gccggaaacg ucuauaauac aaaguucacc     3060

gagaaacccu accgguaucu ccaggaaauc aaggagaacu acgaugugaa aaagauuuau     3120

aauuacgaca ucaaaaacgc uugggacaag gaaaauuccc uggaaaucgu gaagaaaaac     3180

auggagaaga auaccgucaa cauuacacgc uuuaucaaag aagagaaggg ccaacucuuc     3240

gaucugaauc cuauuaaaaa gggggaaacc ucuaacgaga ucauuagcau caagcccaaa     3300

guguauaacg gaaaggacga uaaacuuaau gagaaguacg gcuacuauaa aucccugaac     3360

cccgcguacu uccucuaugu ugaacacaag gagaagaaua aaagaaucaa gucuuuugaa     3420

cgggugaacc uggucgacgu gaauaacauu aaagaugaaa agagccuggu uaaauaccuu     3480

aucgagaaua agaagcuggu ggaaccucgc gucauuaaaa agguguauaa aagacagguu     3540

auccucauua accauuaccc guauuccauc gugacacugg acucuaauaa gcucauggau     3600

uucgagaacc ugaaaccccu uuuucuggaa aauaaguacg agaaaauccu caagaacguc     3660

auuaaguucc uggaagacaa ucaggggaaa agcgaggaga acuacaaguu uaucuaucug     3720

aaaaagaaag aucgguacga aaagaaugag acgcuugaau ccgugaagga ccgcuauaac     3780

cuggaguuca acgaaaugua cgauaaauuu cucgagaagc uggacagcaa agauuauaag     3840

aauuacauga acaauaaaaa guaucaggag cuccuggacg ugaaggaaaa auucauuaaa     3900

cuuaaccugu uugacaaagc cuucacccug aagucuuuuc ucgaucuguu caauagaaaa     3960

acaauggcug acuuuuccaa ggucggacuu accaaguacc ugggcaaaau ccagaagauu     4020

agcucuaacg uucucuccaa aaaugagcug uaccuccugg aagagagcgu gacagggcuu     4080

uucgugaaga aaaucaagcu guga                                            4104


<210>  4256
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4256
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagaccu cgauuacaau guucugagau ucaacaaaaa ggacaugugg      120

ggcucucggc uuuuugauga ggccaaaacg gcugccgaac gcagagugca acggaauagc      180

cgcagacggc ugaagcgcag aaaguggcgg cucaaccugc uugaggaaau uuucuccgac      240

gagaucauga aaauugauuc uaacuuuuuc cgcagacuga aggaaagcuc ccucuggcug      300

gaggacaaaa auucuaagga gaaauuuacc cuguucaaug augacaacua uaaggauuac      360

gacuuuuaua agcaguaccc cacaaucuuc caccuccggg augaacugau uaaaaauccu      420

gagaagaaag acauccgccu uaucuaucug gcgcuccaua gcauuuuuaa guccagaggg      480

cacuuccugu uugaaggaca gaaccuuaaa gagaucaaga auuucgagac ccuguacaac      540

aaucucauua gcuuccugga agacaacggc aucaauaaau cuauugauaa ggacaacauc      600

aagaaacugg agaagauuau cugugauucc gggaaaggac ucaaggacaa agaaaaggag      660

uuuaaggaaa ucuucaacag cgauaaacag cugguggcua uuuuuaaacu uucugucggc      720

uccagcgugu cucugaauga ccucuucgau acagacgagu acaaaaagga ggaaguugag      780

aaagaaaaga ucuccuuucg ggagcaaauu uaugaagaug acaagccgau cuacuauagc      840

auucugggcg agaaaaucga gcuucuggau aucgccaagu ccuucuacga cuuuauggug      900

cucaacaaua uucugucuga cagcaacuau aucuccgaag ccaaagucaa gcuguacgag      960

gaacauaaaa aggaucucaa gaaucugaaa uauauuaucc gcaaguacaa caaagagaau     1020

uacgacaaac uuuucaagga uaagaacgaa aauaacuauc ccgcuuacau ugggcugaau     1080

aaagagaagg gaaagaaaga ggugguugaa aagagcggcc ucaaaaucga cgaucugauc     1140

aaggugauua aaggguaucu uccuaagccc gagagaaucg aagagaagga caaaaccauu     1200

uuuaacgaga uccugaauaa gauugaacuc aaaacaaucc ugccuaagca gcggauuucu     1260

gauaacggaa cccugcccua ccagauccac gaggucgaac ucgagaaaau ccuggaaaau     1320

caguccaagu auuacgacuu ccuuaacuau gaggagaacg gcgugagcac gaaggauaaa     1380

cugcucaaga ccuuuaaauu ccggauuccu uacuacgugg ggccgcuuaa uucuuaucau     1440

aaggacaaag gaggcaacag cuggaucguc cgcaaggaag agggcaagau ucugcccugg     1500

aauuuugaac aaaaaguuga uaucgagaag uccgccgaag aguucauuaa acggaugaca     1560

aacaagugca ccuaccuuaa uggggaggac gugaucccua aagauucuuu ccucuauagc     1620

gaauacauca uucugaacga gcugaauaag gugcagguca acgacgaauu ucucaaugag     1680

gaaaacaaaa gaaagaucau ugacgagcug uucaaggaga auaaaaaggu guccgagaaa     1740

aaguuuaaag aguaucuucu gguuaaccag aucgcuaauc gcacagugga acucaaggga     1800

auuaaggaua gcuucaacuc uaauuacguc uccuauauca aauuuaagga caucuucggc     1860

gagaaacuga accuggauau uuacaaggag aucagcgaaa aaucuauucu uuggaagugu     1920

cucuacgggg acgauaagaa aaucuuugag aagaaaauua agaacgaaua uggagacauc     1980

cugaauaaag augagaucaa gaagauuaac uccuucaaau uuaauaccug gggccggcug     2040

agcgaaaagc uccugacggg gaucgaguuc auuaaccuug agacaggaga augcuacucc     2100

ucugugaugg aggcccugag acgcaccaau uauaaccuca uggaacugcu gagcuccaaa     2160

uuuacacuuc aggagucuau cgacaauggc aacaaggaaa ugaaugaggu uagcuaccgg     2220

gaucucauug aggaauccua ugugagcccc ucucugaaaa gagcgauccu gcaaacccuc     2280

aagauuuacg aggaaaucaa gaaaaucacc ggccgcgucc cuaagaaagu guucauugag     2340

auggccagag ggggagacga aacaaugaag aacaaaaaga uccccgcuag acaggagcag     2400

cugaaaaagc uguaugauuc cuguggcaau gacauugcca acuuuagcau cgauauuaag     2460

aaaaugaagu cuucccugag cucuuacgac aauaacagcc uccgccaaaa gaagcuguac     2520

cuguauuacc uucaguucgg gaaaugcaug uauacgggac gggagaucga ccucgauaga     2580

cugcugcaga auaacgacac cuacgauauc gaccacauuu auccgcgguc caaggucauc     2640

aaggaugaca gcuucgauaa ccucgugcug guucuuaaaa augaaaacgc ugagaagucu     2700

aaugaauacc cugugaaaaa ggagauucag gagaaaauga aguccuuuug gcgcuuccug     2760

aaggagaaaa acuuuaucag cgacgagaag uauaaaagac ucacaggcaa ggaugacuuc     2820

gaacugcggg gguuuauggc cagacaacug gucaaugugc gccagaccac aaaagagguu     2880

ggcaagauuc uucagcagau cgaacccgag aucaagauug uguacuccaa agcggagauc     2940

gccucuagcu uccgggaaau guuugauuuc auuaagguca gagagcugaa cgacacccac     3000

caugcuaaag acgccuaccu caauaucgug gcuggaaacg uguauaauac gaaguuuaca     3060

gaaaaaccuu accgcuaucu gcaagagauu aaggaaaacu acgauguuaa aaagaucuau     3120

aauuacgaca uuaagaacgc cugggauaaa gaaaauuccc ucgagaucgu caagaaaaac     3180

augaagaaaa auaccgugaa caucaccaga uucauuaagg aagagaaggg ccagcuguuu     3240

gaccuuaauc ccaucaaaaa gggggaaaca ucuaacgaga uuaucagcau uaaacccaag     3300

cuguauaacg gaaaagaaga gaagcucaau gagaaguacg gcuacuauaa aucccugaac     3360

ccugccuacu uccuguaugu ggaacacaag gagaaaaaua agcggaucaa aucuuucgaa     3420

cgcgucaacc uuguggaugu uaauaacauc aaggacgaaa agagccuggu gaaauaccuc     3480

auugagaaua agaaacuggu cgaaccgaga cucaucaaga aaguguauaa gcggcagguu     3540

auucugauca acgauuaccc cuauuccauu guggcucuug acagcaauaa acugauggau     3600

uuugagaacc ucaagccucu guuccuggaa aauaaguacg agaaaauccu uaagaacguc     3660

aucaaauuuc uggaagacaa ucaggggaag ucugaggaga acuacaaauu cauuuaucuc     3720

aagaagaaag aucgcuacga aaagaaugag acccuggaau ccgugaaaga cagauauaac     3780

cucgaguuua acgaaaugua cgauaaguuc cuggagaaac uugacagcaa ggacuauaag     3840

aauuacauca acaauaaaaa guauaaaaac cugcuggaug uuaaggagaa auuuauuaag     3900

cucaaucugu ucgacaaggc guuuacacuu aaaucuuucc uggaucucuu uaaccggaag     3960

acgauggccg acuucuccaa agugggacug accaaguacc ucggcaaaau ccaaaagauu     4020

agcucuaaug uccuguccaa gaacgaacuu uaccugcugg aggaaagcgu gacagggcuc     4080

uuugugaaaa agaucaaacu guga                                            4104


<210>  4257
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4257
augaagaaac agcaauucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagacuu caauuacaac guucucagau uuaauaagaa agauaugugg      120

ggcucucggc uguucgacga ggccaagacg gcugccgaac gcagagugca gcggaacagc      180

cgcagacggc uuaaacgcag aaaguggcgg cugaaucucc uggaggaaau uuuuagcgau      240

gagauccuua agauugacuc uaacuucuuu cgcagacuga aagaaagcuc ccucuggcug      300

gaggauaagu cuagcaaaga gaaguucacc cuguuuaaug acgauaacua uaaagacuac      360

gauuucuaua agcaguaccc cacaaucuuu caccuccgga augaacugau uaagaacccu      420

gagaaaaagg acauccgccu uguguaucug gcgcuccacu ccaucuucaa aagcagaggg      480

cacuuucugu ucgaaggaca gaaucuuaag gauauuaaaa acuucgagac ccuguacaau      540

aaccucaugg cuuuucugga ggacaaugac aucuacaaga acauugauuc uuccuauauc      600

ggcaaccugg aaaauauuau cugugacagc aaaaaggggc ucaaggauaa agagaaggag      660

uucaaagaga uuuuuaacuc ugacaagcaa cuggucggau ucuuuaaacu uuccgugggc      720

agcucuguuu cccugaauga ucucuucgac acagaugaau acaagaaggg cgagguggag      780

aaagaaaaga ucagcuuucg ggagcagauc uaugaagacg auaaaccgau uuacuauucc      840

auccuggggg agaagauuga guuccuugac aucgccaaau cuuuuuacga uuucaugguc      900

cugaacaaua uucucgccga cagccaguau aucuccgaag cuaaggugaa gcuguacgac      960

gagcauaaac gcgaucugaa gaaccucaaa uacaucauua gaaaguauaa uaaagaaaac     1020

uacgacaagc uguuuaagga uaaaaaugag agcaacuauu cugccuacau cggacuuaau     1080

aaggaaaaag gcaagaaaga gguuauugaa aagucccggc ugaaaaucga cgauuucgcu     1140

aagauuauca agggguaucu ccccaaagcc gagaagaucg acgagaaaga uagaagcauu     1200

uuuaacgaaa uccuggacaa gauugagcuu aaaaccaucc ugccuaagca gcgcauuucu     1260

gauaauggaa cacuccccua ccaaauccac gaagcggagc uggagaagau ucuggaaaac     1320

caggccaaau auuacgacuu ccucaaucau gaggaaaacg gcaucagcac caaggauaaa     1380

cugcuuauga cguucaaguu ucggaucccu uacuaugugg ggccccugaa cuccuaccac     1440

aaaaauaagg gaggcaacuc uuggauuguc agaaaggagg aaggcaaaau ccucccuugg     1500

aauuucgagc agaaggugga cauugagaaa agcgcugaag aguuuaucaa gcgcaugacc     1560

aacaaaugca cauaucugaa uggggaagau gugauuccga aggacuccuu ccuguacagc     1620

gaguauauca uccuuaacga acucaauaag guccagguua acgacgaguu ucugaauaaa     1680

gagauuaaga aaaagaucau ugaagaucug uucaaaaagu cuaaaaagau cuccgagaag     1740

aacuuucggg aauaccuccu ggugaaucaa auuaccaaca aaacagugga gcuuaaggga     1800

gucaaagacg ccuucaauag caacuaugug ucuuacauca aguuuaaaga uaucuucggc     1860

gacaagcuga aucucgauau uuacaaggaa aucuccgaga aaagcauucu guggaagugu     1920

cuguaugggg acgauaaaaa gaucuuugag aaaaagauua aguccguuua cggagacauc     1980

cuuaccaaag augaaaucaa gaaaauuaac ucuuucaagu uuaacacgug gggcagacuc     2040

agcgagaaac ugcugacaga aaucgaguuc auugaccucg agaccgggga augcuauucc     2100

ucugugaugg augcucugcg ccggacaaau uacaaccuua uggagcugcu cagcuccaag     2160

uucacccugc aggaaaauau cgacaacgag aauaaggaag ucagcgaguu uucuuauaga     2220

gaucuggugg aggaauccua cguuagcccc ucucuuaaac gcgccauucu ccagacccug     2280

aagaucuaug aggaaauucg gaaaaucaca ggaagaaucc cuaagaaagu guucauugag     2340

auggcgcgcg gcggcgacga aacgaugaag aacaagaaaa uccccgcccg gcaagagcag     2400

cugaagaaac ucuacgacuc cugugggaag gauauuagca auuuuucuau cgacauuaaa     2460

gagaugaaga acagccuuaa uuccuacgau aacaauagcc ugagacagaa aaagcuguau     2520

cucuacuauc ugcaguucgg aaagugcaug uacaccggca aagaaaucga ccugaaccgg     2580

cuucuccaaa auaacgauac auaugacauc gaucacauuu acccucgcuc uaaggucauc     2640

aaagacgauu ccuuugacaa ucuggugcug guccucaaga acgagaacgc ugaaaaaucc     2700

aaugaguauc cccugaagaa ggaaauucag gagaaaauga aguccuucug gaaauuucuu     2760

aaggagaaaa acuucaucuc ugaugaaaag uacaagagac ugaccgggaa agacgaguuu     2820

gaacuccggg gauucauggc cagacagcug gugaauguuc gccagacaac caaggaggcu     2880

ggcaaaauuc ugcaacagau cgagccggaa aucaagauug uguacagcaa agccgagauc     2940

gccuccucuu uucgggaaau guucgacuuc auuaagguca gagagcuuaa cgauacgcau     3000

cacgcuaagg acgcguaucu gaauaucgug gccgggaacg uuuacaauac aaaauuuacc     3060

gaaaagccuu aucgcuaccu ccaggagauu aaagagaacu augaugugaa gaaaaucuac     3120

aauuaugaca uuaagaacgc uugggauaaa gaaaagagcc uggagaucgu caagaaaaau     3180

auggaaaaga acaccgugaa uaucacaaga uucauuaaag aggaaaaggg ccagcucuuu     3240

gaccugaacc ccauccggaa aggagagacc uccaaugaga uuaucgccau uaagccuaag     3300

uucuacgaag gcucuacaga gaaacuuaac gaaaaguacg gguauuacaa aagccugaau     3360

cccgccuauu uuaucuacgu ggagcauaag gagaaaaaca agaucauucg cuccuucgaa     3420

agaguuaacc ucgucgaugu gaauaagauc aaagacgaaa agagccugau uaaauaucug     3480

aucgaaaaca agggacuuau ugagcccaaa gugaucaaga aggucuacaa acggcaagug     3540

auccugauua auaacuuucc uuauucuauc guugcucucg auuccaauaa gcugauggac     3600

uucgaaaacc ucaaaccgcu guuucuugag cgcaaguacg agaaaauucu gaagaaugcc     3660

aucaaauucc ucgaagauaa ccagggcaag acggaggaau acuauaaguu ucuguaccug     3720

aaaaagaaag acagaaauga gaagaacgaa accauugaua gcgugaaaga gcgguauaau     3780

aucgaguuca acgaaaugua cgacaaguuu cuugagaagc uggacucuaa agauuauaag     3840

aauuacauua acaauaaaaa guauuccgaa cucgucaacg ugaaagagaa guucaucaag     3900

cugaaccucu ucgacaaagc guuuacacuu aagagcuucc uggaucuguu uaaucgcaaa     3960

accauggcug acuucucuaa gguugggcug acaaaauacc ucggaaagau ccagaagauu     4020

uccagcaacg ugcuguccaa aaaugaacuu caucugcucg aggagagcgu caccggccug     4080

uuugugaaga aaaucaagcu cuga                                            4104


<210>  4258
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4258
augaaggacu acuaucuggg ccucgauaug gggagcuccu cugugggaug ggcccugagc       60

gacgagagau acaaccuucu gcgggcuaaa ggcaaggauc ucuggggagu ccgccuguuc      120

gacgaagcca aaaccgcggc ugagagacgg acauuucgcu cccagagacg gcgccuugaa      180

agaucuaagu ggcggcugag ccuccugacg ucccuguucg aggaagagau cucuaaaauu      240

gaugacaagu uuuucgaucg ccucaaggac agcaaauuuu ucaucgauga caaguccgau      300

caacagccca auacccuguu uaacgacguu aauuucacag auaaagagua ucacaagaga      360

uacccuagca uuuaucaucu ucggaacgcc cuguuuaauc agaacgacaa auucgacauc      420

cgccucauuu uucuggcccu uucucacauc gugaagaaga gagggcauuu ccuguuugau      480

ggcgacaaag cuggguucgg agaagauauc gacgugauuc ucgaugagcu gaaggccuac      540

cugcaggacg cuuuccacac caaacucgaa uuuacauccg aguucaagag caucaugcug      600

gauaaaaauc uucggaagaa acaaaaggag gaagaacuga agaacucuau uaaaaucucc      660

agcgacacca uuaauucuaa ggauggcaaa uccauccuca uuaagcuguu ucuuggcuau      720

gaagccaacc ugaaaccguu cuuucucgag uacaaggucg aaaaggacaa ugaguacaaa      780

aucaacuuca gcaaggagga uuaugaagag aaacugcugg acaugacauc caaguuugaa      840

gagaccacgu acuucaucga aauugcgaaa aagauccaug acuauuuucu ccuggaggag      900

auuaugcuug gguucaagaa uaucucugcc agcaaagugu ccauuuacca gaagcacaaa      960

aacgaucuga agacccucaa aaauaucguu aagaaccaga ucaacaauag cuauaacgcu     1020

auuuuuaagg acaucggacu ggauaauuac cccgccuaug ugucuugcug gaacuccaaa     1080

aagcacaaag aaaagcagaa aggcuguaca aaggacaaau ucuacgcuuu ucuuaagacc     1140

aagcugaaaa auauuaacaa ugccaaggag auccucgaua gcauugacaa cgaugacuuc     1200

cugccucgcc uggucgauaa aaauucuauc aucccccauc aaauucaccu cgcggaacug     1260

aucaagauua ucgacaacca gagccaguac uauccuuucc uuaaagagau uaaggauaag     1320

aucauuucca ucuuuaaauu cagaaucccc uuuuacgugg ggccucugaa uaacuuccau     1380

aaggacaaag gaggcaaugc cuggauugaa aagcucaaag augagcggau cuuuccgugg     1440

aacuucaaug acguugugga ccugaacaag ucugcugcca acuuuauuga gaagaugauc     1500

aaugaaugca gcuaucugaa aggggagaag guccuugcua aaaacucccu ccuguacaca     1560

aaguauagcg ugcugaaucu cauuaacaaa cugaagauca auggaaccgc cgugucuguc     1620

gaaacgaagc agaaaaucua caacgagcuu cugcucaagc aaaagaauau uaagaaaaca     1680

gccaucaaga aauuccugau uaacaauucc aucauugaua agaacgacga gaucagcggc     1740

aucgaugacu cucugaccuc caaucuugcu guuuuuauug auuucgaaaa gaucagcaac     1800

agacuccgcu augaccagaa agaggauauu aucaagucca uuucucuguu uggcuacgac     1860

aaugcgaugc ugaaaaagca gaucaucaca auugcccccg aucucagcga agacgagcug     1920

gaaaaacuuu ccaagcugcg guacucuggg uucggaagac ucagcaagcg ccugcugacc     1980

uccaucaaac acaacggcaa gagcauuauc gagaugcuuu gggauaccaa ugacaaccuc     2040

auggagcugc ugucugaucg guauacauuu auugaacaaa ucaacaaaca caaugacggg     2100

uucaaggagg acauuaaccu caauuuccag aacguggaua cgcuguacgu guccccuagc     2160

gucaaaagac agaucuggca ggcuuuuaag gugaucaaug aacuuauuuc uguuauggga     2220

cgcgagccca agaaaaucuu cguggaaaug gcccgggagg agggcgaaaa gaaaagaacc     2280

caagcccgca aggacaaagu ccuggagcuc uauaagucca uuaagaacaa ugaaaucagc     2340

acauuucuga ccaacuucaa agauauuaag acaaaacugg agggggaauc uaaucuuaac     2400

agcaagaaac ucuaccugua uuaccugcag cucggagugg acaucuauac cggcgagaag     2460

aucccuuuug agaagcuguu cgaucagaau auuuacgaca ucgaucaugu uauuccccgg     2520

ucccuuaaaa aggacgauag cuuugacaac cuggugcuca cgucuaaaac aaauaacgcu     2580

aagaaaaccg augaguuccc ggucuccagc gagauccagu ccaagaugca aaccuuuugg     2640

aaagugcuga aggacaagga ccugaugucu gaaauuaaau acacaaaucu uaccagaaag     2700

acacaccuga gcgaugacga gcucggcgau uucaucgccc gccagcuggu cgagacgcgg     2760

caguccacca aagcgcucac acagauccug aagaacgugu augcuaacuc ugacauuguu     2820

uacgugaaag ccgggaucgu cagcgaauuu agacaacagu ucgauaugcu uaagauucgg     2880

gagcugaaug acuuccauca cgccaacgau gcuuaucuca auaucgugau uggaaacguu     2940

uacaauauca aguuuaccaa aaacccucgc aauuauguga aggaauacua uaaaaagggc     3000

gagcauuaca accugaaaau ugaaaagaug cuggagaagg acaucgccag agggggaaau     3060

uucggcuggg auaaaaagca cuccaucgag acaguccaga aaaccaugaa gaaacagucu     3120

ccccuuauua cguaccggac cuuugaaaac aagggggagu ucuuuaauca acagccuagc     3180

aaggcuuccg ccaaccugcu cgcgcugaaa agcucugaca agagacuccu ggauacagug     3240

cgcuauggcg gauacaaagg cacccaguau gcccacaugu uccuugugga acauuuuaau     3300

gggaagauca uuaaagagau caugggaguu cccauuuacc uguccaagga aaucaaagac     3360

gauaaggcua agcuccugga guauuguaac gacguccuga aacuuaagaa ucccaaaauc     3420

cuguucagca agauuccuua caacucucuc aucguguaua auggcuaccc gauucugcuc     3480

cgggggcuga caggagugag aggcgccgcu caacuuaaac uggaccagac cuccacgcag     3540

gucgugaaga acaucguuaa gcuguacgau uggcucagcc agaaacaaaa ggagcugucu     3600

gaccuuucca aaaagauuga auuuuuccag gagaacaaua aaaaggauga agugaaggcc     3660

gcgaugcugg aggccgacaa cauccucuuu auccugaaug aucgcuucaa aaacacaaag     3720

auugggaaag acaucaugaa ugauaaccac cucaauaacc uguaugacac cuuuauuaau     3780

aaacuugaaa acagcaucua caaaaauaag cccggcggaa agcaggaugu ccugaugaaa     3840

gagaagagag accuguuccu cuccuucagc augucuaaaa agugcauuac acuguauaaa     3900

aucauuaaga ucuuuggcag cugugaucag gggguggacu ucuccgauau cgcugagaaa     3960

auuucuaaga gcggaggcau cgggggaaag aaccuuggca aaaauaccuu ugaccugguu     4020

uucaaggaaa acuccaccua cacacucgug caaucuagcg ucacggggcu guaugccaaa     4080

aagaccgacc uccauaaacu guga                                            4104


<210>  4259
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4259
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagaccu cgauuacaau guucugagau ucaacaaaaa ggacaugugg      120

ggcucucggc uuuuugagga agccaaaacg gcugccgagc gcagagugca acggaauagc      180

cgcagacggc ugaagcgcag aaaguggcgg cucaaccugc uugaagagau uuucuccaau      240

gaaauccuga aaauugauuc uaacuuuuuc cgcagacuca aggagagcuc ccuguggcug      300

gaggacaaau cuagcaagga aaaauuuacc cucuucaaug augacaacua uaaggauuac      360

gacuuuuaua agcaguaccc cacaaucuuc caucugcgga augagcuuau uaaaaacccu      420

gaaaagaaag auauccgccu gguguaucuc gcgauccacu ccauuuuuaa gagcagaggg      480

cacuuccugu uugagggaca gaaucuuaaa gagaucaaga acuucgaaac ccuguacaau      540

aaccucauug cuuuccugga ggacaauggc aucaacaaaa uuaucgauaa gaacaauauu      600

gaaaagcugg agaaaaucgu cugugacucu aagaaagggc ucaaggacaa agaaaaggag      660

uuuaaggaga ucuucaacuc cgauaaacag cugguggcca uuuuuaaacu uagcguugga      720

ucuuccguga gccugaauga ccucuucgau acagacgaau acaaaaaggg cgaggucgaa      780

aaagagaaga ucucuuuucg ggaacaaauu uaugaggaug acaagccgau cuacuauucc      840

auucugggcg agaaaaucga acuucuggau aucgccaaga ccuucuacga cuuuauggug      900

cucaacaaua uucuggcuga uagccaguau aucuccgagg ccaaaguuaa gcuguacgag      960

gagcauaaaa aggaccucaa gaaccugaaa uauauuaucc gcaaguacaa uaaagggaac     1020

uacgauaaac uuuucaaaga caagaaugaa aacaauuauu cugcuuacau uggacugaac     1080

aaagaaaaga gcaagaaaga ggugaucgaa aaguccagac ucaaaaucga cgaucugauu     1140

aagaauauca aaggcuaucu ucccaagguc gaggaaauug aggagaagga caaagccauc     1200

uuuaacaaga uucugaauaa aaucgaacuc aagacaauuc ugccuaaaca gcggaucagc     1260

gauaacggga cccugcccua ccagauccac gaggcggaac ucgagaagau ucuggaaaac     1320

caaucuaagu auuacgacuu ccuuaauuau gaggagaacg gaaucauuac gaaagauaag     1380

cugcucauga ccuuuaaauu cagaaucccu uacuacgugg gcccccuuaa uuccuaucau     1440

aaggacaaag ggggaaacag cuggauugug cgcaaggaag agggcaagau ccugccuugg     1500

aauuuugaac agaaagucga uaucgagaag ucugccgaag aguucauuaa acggaugaca     1560

aacaagugca ccuaccugaa uggcgaggac guuaucccga aagauacauu ccucuauagc     1620

gaauacguga uucugaacga gcugaauaag gugcagguca acgacgaauu ucucaaugag     1680

gaaaacaaaa gaaagaucau ugaugagcug uucaaggaga auaaaaaggu guccgaaaag     1740

aaguuuaaag aguaucuucu gguuaagcag aucguggacg ggaccaucga acucaaggga     1800

gucaaagauu cuuucaacag caauuacauu uccuauaucc gcuuuaagga cauuuucggc     1860

gagaaacuga accuggacau cuacaaggag auuagcgaaa aaucuauccu uuggaagugu     1920

cucuacgggg augacaagaa aaucuuugag aagaaaauua agaacgaaua uggagauauc     1980

cugacgaaag acgagauuaa gaagaucaau acauucaaau uuaacaauug gggccggcug     2040

uccgaaaagc uccugaccgg gauugaguuc aucaaccuug agacaggaga augcuacagc     2100

ucugugaugg augcucugag acgcaccaau uauaaccuca uggagcugcu guccagcaaa     2160

uuuacccuuc aagaauccau uaauaacgag aauaaggaaa ugaacgaggc cucuuaccgg     2220

gaccucaucg aggaaagcua uguuuccccc ucucugaaaa gagcuaucuu ccagacacug     2280

aagauuuacg aggaaauccg caagauuacg ggccgggugc cuaaaaaggu cuuuaucgag     2340

auggccagag gcggggauga aagcaugaaa aauaagaaaa uucccgcgcg ccaggagcaa     2400

cucaagaaac uguaugacuc cuguggaaac gauaucgcca auuucagcau cgacauuaag     2460

gagaugaaga acucucuuau cuccuacgau aauaacagcc ugcggcagaa aaagcucuac     2520

cuguauuacc ugcaguucgg caaaugcaug uauaccggga gagaaauuga ccuugaucgg     2580

cuccugcaga acaaugacac auacgacauc gaucacauuu auccucgcuc uaaggugauc     2640

aaagacgauu ccuuugacaa ccugguccuc gugcugaaga augagaacgc ugaaaagagc     2700

aaugaguacc ccguuaaaaa ggagauccaa gaaaagauga agucuuucug gagauuucuu     2760

aaagagaaga acuucauuag cgaugaaaag uauaaacggc ugaccggaaa ggacgauuuu     2820

gagcucagag gcuucauggc ccgccagcug gugaaugucc ggcagacaac caaagaagug     2880

gggaagaucc ugcagcaaau ugagccggag aucaaaauug uuuacuccaa ggcugaaauc     2940

gccagcucuu uuagagagau guucgacuuu auuaaggugc gcgaacuuaa cgauacgcac     3000

caugccaaag acgcuuaccu gaauaucguc gcgggcaacg uguauaauac aaaguucacc     3060

gagaaaccuu acagauaucu ccaggaaauc aaggagaacu acgaugugaa aaagauuuau     3120

aauuacgaca ucaaaaacgc cugggacaag gaaaauuccc uggaaauugu uaagaaaaac     3180

auggagaaga auaccgucaa caucacacgg uuuauuaaag aaaagaaagg acaacucuuc     3240

gaucugaacc ccaucaagaa gggcgagacc agcaaugaaa ucauuuccau caaaccuaag     3300

guguauaacg ggaaagacga uaaacuuaau gagaaauacg gauacuauaa gucucugaac     3360

cccgcuuacu uccucuaugu ggagcacaag gagaaaaaua agcgcauuaa gagcuuugag     3420

agagucaacc ugguggacgu uaauaacauc aaggaugaaa aaucccuggu gaaguaccuu     3480

auugagaaua agaaacuggu cgagccccgg gugaucaaga aaguuuauaa gcgccaggug     3540

auccucauua acgacuaccc uuauucuauc gucacacugg auagcaauaa acucauggac     3600

uucgaaaacc ugaagccgcu uuuucuggag aauaaauacg aaaagauucu caagaacgug     3660

aucaaauucc uggaggauaa ucagggcaag uccgaagaga acuacaaauu uauuuaucug     3720

aagaaaaagg acagauacga gaagaacgaa acgcuugagu cuguuaaaga ucgguauaau     3780

cuggaguuca acgaaaugua cgacaaguuu cucgaaaaac uggauagcaa ggacuauaaa     3840

aauuacauga acaauaagaa guaucaggag cuccuggacg ugaaagagaa guucaucaaa     3900

cuuaaccugu uugauaaggc cuucacccug aaauccuuuc ucgaccuguu caaucgcaag     3960

acaauggccg auuuuagcaa ggucgggcuu accaaauacc ugggaaagau ucagaaaauc     4020

ucuuccaacg ugcucagcaa gaaugaacug uaccuccugg aggaaucugu gacaggccuu     4080

uucgucaaaa agaucaagcu guga                                            4104


<210>  4260
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4260
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagacau gaauuacaac guucucagau ucaauaaaaa ggauaugugg      120

ggcucucggc uguuugagga agccaaaacg gcugccgagc gcagagugca acggaacagc      180

cgcagacggc uuaagcgcag aaaguggcgg cugaaucucc uggaagagau uuucuccaac      240

gaaauccuua aaauugacuc uaacuuuuuc cgcagacuga aggagagcuc ccucuggcug      300

gaggauaaau cuagcaagga aaaauuuacc cuguucaaug acgauaauua uaaggacuac      360

gauuuuuaua agcaguaccc cacaaucuuc caccuccgga acgagcugau uaaaaauccu      420

gaaaagaaag acauccgccu uguguaucug gcgauccauu ccauuuuuaa gagcagaggg      480

cacuuccucu uugagggaca gaaccugaaa gagaucaaga auuucgaaac ccuuuacaac      540

aaucugauug cuuuccucga ggauaacggc aucaacaaau cuauugacaa ggauaaucug      600

gaaaagcugg agaaaaucgu cugugacucc gggaagggac ucaaagacaa ggaaaaagag      660

uuuaaggaga uuuucaacag cgauaagcag cugguggcca ucuuuaaacu uucuguuggc      720

cuguccguga gccucaauga ccuguucgau acagacgaau acaagaaagg cgaggucgaa      780

aaggagaaaa ucucuuuucg ggaacaaauu uaugaggaug acaagccgau cuacuauucc      840

auucuugggg agaagaucga acugcucgau auugccaaga gcuucuacga cuuuauggug      900

cugaacaaua uccuggcuga uucccaguau aucucugagg ccaagguuaa acucuacgag      960

gagcauaagc gcgaccugaa aaaccuuaag uauauuauca gaaaguacaa ucgggaaaac     1020

uacgauaaac uguucaagga caaaaaugag agcaacuauc ccgcuuacau uggacucaau     1080

aaggagaaau ccaagaaaga agugaucgaa aagagcaagc ugaaaauuga cgaucuuauc     1140

aagucuauca aaggcuaucu gccuaagguc gaagagauug aggaaaaaga caaggccauc     1200

uuuaacgaga uucucaauaa gaucgaacug aaaauuaucc ugcccaagca gagaauuucc     1260

gauaacggga cccucccuua ccagauccac gaggcggaac uggagaaaau ccuugagaau     1320

caaagcaagu auuacgacuu ccugaacuau gaagagaacg gaauuaucac aaaagauaag     1380

cuccugauga ccuuuaaguu ccgcauuccc uacuacgugg gcccucuuaa uucuuaucau     1440

aaagacaaag ggggaaacag cuggaucgug cggaaagaag agggcaagau ucugccgugg     1500

aauuuugaac agaaagucga uaucgagaag uccgccgagg aguucaucaa gagaaugacg     1560

aacaaaugca ccuaccucaa uggcgaggac guuauuccca aggauucuuu ccuguauagc     1620

gaauacauca uucugaacga gcucaauaaa guccagguga acgacgaauu ucugaaugag     1680

gagaacaagc gcaaaaucau ugaggaacuu uucaagggga auaaaaaggu guccgaaaag     1740

aaauuuaagg aguaucugcu cgugaaccag aucacaaccg gaacaaucga gcugaaaggc     1800

auuaaggaua gcuucaauuc uaacuacauc uccuauauua aauuuaagga caucuucggg     1860

gaaaagcuga aucuugauau uuacaaagag aucagcgaaa agucuauccu cuggaaaugu     1920

cuguacggag acgacaagaa aauuuuugag aagaagauca aaaacgaaua uggcgauauu     1980

cugaccaagg acgagaucaa aaagauuaac acguucaaau uuaauaacug ggggcggcuc     2040

uccgagaagc ugcuuacagg aaucgaguuc auuaaucugg aaaccggcga augcuacagc     2100

uccguuaugg aggcucucag acgcacaaac uauaaucuga uggaacugcu uucuagcaag     2160

uuuacccucc aagagaacau cgauaaugag aacaaagaca ucucccaguu cucuuaccgg     2220

gaucuggugg aagagagcua ugucuccccu agccugaaga gagccauucu ccagacccug     2280

aaaaucuacg aagagauuaa gaaaaucaca ggccgcgugc ccaagaaggu uuuuauugaa     2340

auggcuagag ggggagacga gucuaugaaa aauaagaaaa ucccugcccg gcaagagcag     2400

cuuaagaaac uguaugauuc cuguggcaac gacaucgcga auuucagcau ugauaucaag     2460

gaaaugaaau cuucccucag ccuguacgac aacaauucuc ugcgccagaa gaaacuuuac     2520

cucuauuacc ugcaguucgg gaaaugcaug uauacgggac gggagauuga ucuggacaga     2580

cuccugcaaa acaaugauac cuacgacauc gaccacauuu auccccggag caaggugauc     2640

aaagaugacu ccuuugauaa ccuuguccug gugcucaaga acgaaaaugc cgagaaaagc     2700

aacgaguacc cggucaagaa ggaaauccag gagaaaauga agucuuucug gcgcuuucug     2760

aaagaaaaga auuucauuuc cgacgagaaa uauaagagac ugacaggcaa ggaugacuuu     2820

gaacuucggg gguucauggc uagacagcug gugaacguuc gccagaccac aaaagaggug     2880

ggcaagaucc uccaacagau ugagccugaa aucaaaauug ucuacagcaa ggccgagauc     2940

gcuuccucuu uucgggaaau guucgauuuu auuaaaguga gagagcugaa ugacacccac     3000

caugccaagg augccuaccu caacaucguu gcuggaaaug uguauaacac gaaguucaca     3060

gaaaaacccu accgcuaucu gcaggagauc aaggagaauu acgacgucaa aaagauuuau     3120

agcuacgaua ucaaaaacgc gugggacaag gaaaauuccc uugagauugu gaaaaagaac     3180

auggaaaaga auaccgugaa caucaccaga uuuauuaaag aggaaaaggg ccagcuguuc     3240

gaccucaauc cuaucaaaaa gggggagaca ucuaacgaga ucauuagcau caaacccaag     3300

guuuauaaug gaaaggauga caaacugaac gaaaaguacg gcuacuauaa aucccugaac     3360

cccgccuacu uccuuuaugu cgagcacaag gaaaagaaua agcggauuaa gucuuuugag     3420

cgcgugaacc ugguggaugu caauaacauc aaagacgaaa agagccucau uaaauaccug     3480

guggaaaaua agaaacucgu ugagccuaga gugaucaaga aggucuauaa acggcaagug     3540

auccugauua acgauuaccc guauuccauc guugcucuug acagcaauaa gcugauggau     3600

uucgaaaacc ucaaaccccu guuucuggag cgcaaguacg agaaaauucu uaagaaugug     3660

aucaaauucc uggaagacaa ccaggggaag ucugaggaaa auuacaaguu uauuuaucuc     3720

aaaaagaaag auagauacga gaagaacgaa acccuggagu ccguccggga acgcuauaau     3780

aucgaguuca acgagaugua cgacaaauuu cucgaaaagc ugaauagcaa ggauuauaaa     3840

aacuacauua acaauaagaa auaucaggac cuucuggaug ugaaggagaa auucaucaag     3900

cugaaccucu uugacaaggc cuucacacug aaaucuuuuc uugaccuguu caauagaaag     3960

acgauggccg auuuuuccaa aguuggacuc accaaguacc ugggcaaaau ccagaagauu     4020

agcucuaacg ugcucuccaa gaaugaacug uaccuucugg aggaaagcgu cacagggcug     4080

uucgugaaaa agaucgagcu cuga                                            4104


<210>  4261
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4261
auggugaaga aaaaguacgc caucggcauu gacaucggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa agugcccucu aagaaaauga aggucuucgg caauacggag      120

aagagauaca uuaaaaagaa ccugcucggg acccugcuuu uugaugaagg aaauacagcu      180

gagaaccggc gccugaaaag aaccgcccgg cgcagauaua cacggcgcag aaaucggauc      240

cucuaccugc aggaaauuuu cgcggaggaa aucaacaaga ucgacgauag cuuuuuccaa      300

cgccuugacg auuccuuucu gauuguggag gacaaacagg gcucuaagca cccuaucuuc      360

gggacccucc aggaggaaaa ggaguaucau aaacaguuuc cgacaauuua ccaccugaga      420

aagcaacugg cugauagcuc ccagaaagcc gacauccggc ucauuuaucu ggcccuugcu      480

cauaucauua aguaccgcgg acacuuccug uuugaaggcg aucucaaauc ugagaauaag      540

gacguucagc aucuguucaa cgauuuugug gagauguucg acaaaaccgu cgaaggcagc      600

uaccuuuccg agaaucugcc caacguggcc gacguucucg uggaaaaggu cagcaagucu      660

agacggcugg agaauauccu gcacuauuuu ccuaacgaga aaaagaaugg gcuuuucgga      720

aacuuccuga cgcuugcucu gggccuccag cccaauuuua aaaccaacuu cgaacuggcc      780

gaggaugcga agauccaauu uuccaaagaa acauacgagg aagaccuuga ggaacugcuc      840

gggaagauug gagaugacua ugccgaucug uucaucgcua ccaagagccu guacgacggc      900

auucuccugg ccgggauccu uucuacaacc gauuccacga caaaagcucc ucugucuagc      960

uccaugguga accgcuauga ggagcaccag aaggaccucg cccugcuuaa aaauuuuauu     1020

caucagaacc ugagcgauuc cuacaaggaa guguucaaug acaaacucaa ggauggauau     1080

gcgggcuaca ucgagggcaa gaccacacag gaaaacuuuu acagauucau caaaaaggcc     1140

auugagaaaa ucgaaggguc uaauuauuuu auugacaaga ucgaccggga ggauuuccug     1200

agaaaacaac gcaccuuuga caacggaagc auuccccacc agauccaucu gcaggagaug     1260

cacgcuauca uucggagaca agccgaauuu uacccuuuuc ucgucgagaa ucaggauaag     1320

aucgaaaaga uucugaccuu ccgcaucccg uauuacguug gcccccuugc ucgggggaaa     1380

uccgaguucg ccuggcugaa cagaaagagc gacgagaaaa uucgcccuug gaauuuugau     1440

gaaauggugg acaaggagac aucugccgaa aacuucauca cgcggaugac caauuaugau     1500

caguaccucc ccgaccagaa agugcugccu aagcacuccc ugcuuuauga gaaauuugcu     1560

gucuacaacg aacucacaaa ggugagauac guuaccgagc aaggaaagag cuucuuuuuc     1620

gaugcgaaua ugaaacagga gauuuuugac ggcguguuca aggucuaucg caaagugaca     1680

aaggaaaaac ugauggauuu ucuggggaag gaguucgacg aauuucggau cguugaucuc     1740

cugggacuug acaaggacaa caaaucuuuc aaugccagcc ugggcaccua ccaugaucuc     1800

aagaaaaucg uguccaagga ccugcuggau aaccccgaga acgaagacau ucuugagaau     1860

gucgugcuca cgcugacacu guuugaggau agagaaauga uccgcaaacg gcucgagaag     1920

uauaaggacg uccugaccga ggaacagcgg aaaaagcucg aacggcgcca cuacaccggc     1980

ugggggagac ugucugcuaa acucauuaac ggaauccggg auaaggugac aagaaaaacc     2040

auucuggacu aucugaucga ugacggcaca agcaaucgca acuucaugca gcuuaucaau     2100

gaugacacgc ucuccuucgu ugaugagauu cggcuggccc aagggagcgg agaggccgaa     2160

gacuacagag cugaggugca gaaccuggcc ggcucuccgg cgaucaagaa ggggauucuc     2220

cagucccuga aaaucgucga cgaacuuauu gaggugaugg gcuaugaucc ugagcauauc     2280

guuguggaaa uggcucgcga gaaucaguuu accaaccaag gaagacggaa uagccagcag     2340

cgcuacaaga aaaucgaaaa cgccauuaag aaucuggacu cuaaccucaa uuccaaaauc     2400

cugaaggagu accccacaaa caaucaggcc cugcaaaacg auagacuuuu ccucuauuac     2460

cugcagaacg gcaaagacau guauaccgau gaggaacugg acauugauca gcucagccaa     2520

uacgacaucg aucacauuau cccucaggcu uuuaucaagg acgauucccu ggacaauaag     2580

guccuuacaa aaucugccaa gaaccggggg aaaagcgacg augugcccuc ccuggagauu     2640

gugcauaaga aaaagaauuu cuggaagcag cuccuggacu cucagcugau cagccaacgc     2700

aaauuugaua accuuaccaa ggcugaaaga ggaggccuga cgaaugagga caaagcccgg     2760

uucauucagc gccagcucgu ugaaaccaga cagaucacaa agcacgucgc gcggauucug     2820

gauacccgcu uuaacacaaa acucgacgag gccgggaaua gaauccggga ucccaaggug     2880

aacauuauca cccugaaguc caaucuugug agccaauuca gaaaagacua ucagcuguac     2940

aagguccgcg aaaucaacaa uuaucaccau gcucacgaug ccuaccucaa cgcugugguu     3000

gccacggcgc ugcugaaaaa guacccucag cuugccccgg aguuugugua uggagacuac     3060

cccaaauaua auucuuacaa guccagaaag agcgcuacag agaaaguccu guucuauucu     3120

aacauuauga auuucuucag acgcgugcuc guuuacucca agaccggcga agugcggauc     3180

agaccuguca uugaggugaa caaagaaaca ggggagaucg uuugggauaa gaaaagcgac     3240

uuccgcaccg ugcggaaggu ccugucuuau ccccagguga acguggucaa gaaaguugaa     3300

augcaaaccg gcggauuuag caaggagucc auucuccagc auggcgauag cgacaaacug     3360

aucccuagaa agacagagaa auucuaccuu gacacgaaga aauacggggg auuugauucu     3420

cccaccaucg ccuauuccgu gcugcucauu gcugacaucg aaaagggcaa ggccaaaaag     3480

cugaaacgcg ugaaggagcu gauugggauc acaauuaugg aacggauggc cuucgagaaa     3540

aauccgaucg aguuucuuga acacaaggga uacaagaaca uccuggagaa aaauauuauc     3600

aagcucccua aauauagccu guucgaacuc gagaacggca gacggcgccu gcuugcuucc     3660

gcgaaggaac ugcagaaagg gaaugagaug auucugcccc cucaccucgu cacccugcuu     3720

uaccauucua gcaacaucca caagauuaca gagcccaucc aucugaauua ugugaacaag     3780

aauaaacacg aauuuaagga gcuccugaga cauauuuccg auuucucuac ccgguacauc     3840

cucgcccaag accgccugag caaaaucgag gaacuuuaug auaagaacga cggcgaugac     3900

auuuccgauc ugacgucuag cuuuguuaau cugcucacau ucaccgcuau cggagccccu     3960

gccgcuuuua aauuccuggg cuccgugauu gacagaaagc gguacaccag caucgccgaa     4020

auucuugagg cgacacugau ccaccagucu gucaccgggc ucuacgagac aagaaucgau     4080

cuguccaagc ucggaggcga cuga                                            4104


<210>  4262
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4262
auggugaaga aaaaguacgc caucggcauu gacaucggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa agugcccucu aagaaaauga aggucuucgg caauacggag      120

aagagauaca uuaaaaagaa ccugcucggg acccugcuuu uugaugaagg aaauacagcu      180

gagaaccggc gccugaaaag aaccgcccgg cgcagauaua cacggcgcag aaaucggauc      240

cucuaccugc aggaaauuuu cgcggaggaa aucaacaaga ucgacgauag cuuuuuccaa      300

cgccuugacg auuccuuucu gauuguggag gacaaacagg gcucuaagca cccuaucuuc      360

gggacccucc aggaggaaaa ggaguaucau aaacaguuuc cgacaauuua ccaccugaga      420

aagcaacugg cugauagcuc ccagaaagcc gacauccggc ucauuuaucu ggcccuugcu      480

cauaucauua aguaccgcgg acacuuccug uuugaaggcg aucucaaauc ugagaauaag      540

gacguucagc aucuguucaa cgauuuugug gagauguucg acaaaaccgu cgaaggcagc      600

uaccuuuccg agaaucugcc caacguggcc gacguucucg uggaaaaggu cagcaagucu      660

agacggcugg agaauauccu gcacuauuuu ccuaacgaga aaaagaaugg gcuuuucgga      720

aacuuccugg cucuugcccu gggccuccag cccaauuuua aaacgaacuu cgaacuggcg      780

gaggaugcca agauccaauu uuccaaagaa accuacgagg aagaccuuga ggaacugcuc      840

gggaagauug gagaugacua ugcugaucug uucaucgcca caaagagccu guacgacggc      900

auucuccugg cugggauccu uucuaccaca gauuccacca cgaaagcccc ucugucuagc      960

uccaugguga accgcuauga ggagcaccag aaggaccucg cgcugcuuaa aaauuuuauu     1020

caucagaacc ugagcgauuc cuacaaggaa guguucaaug acaaacucaa ggauggauau     1080

gccggcuaca ucgagggcaa gacaacccag gaaaacuuuu acagauucau caaaaaggcu     1140

auugagaaaa ucgaaggguc uaauuauuuu auugacaaga ucgaccggga ggauuuccug     1200

agaaaacaac gcacauuuga caacggaagc auuccccacc agauccaucu gcaggagaug     1260

cacgccauca uucggagaca agcugaauuu uacccuuuuc ucgucgagaa ucaggauaag     1320

aucgaaaaga uucugaccuu ccgcaucccg uauuacguug gcccccuugc ccgggggaaa     1380

uccgaguucg ccuggcugaa cagaaagagc gacgagaaaa uucgcccuug gaauuuugau     1440

gaaauggugg acaaggagac cucugcugaa aacuucauca cacggaugac gaauuaugau     1500

caguaccucc ccgaccagaa agugcugccu aagcacuccc ugcuuuauga gaaauuugcg     1560

gucuacaacg aacucaccaa ggugagauac guuacagagc aaggaaagag cuucuuuuuc     1620

gaugccaaua ugaaacagga gauuuuugac ggcguguuca aggucuaucg caaagugacc     1680

aaggaaaaac ugauggauuu ucuggggaag gaguucgacg aauuucggau cguugaucuc     1740

cugggacuug acaaggacaa caaaucuuuc aaugcuagcc ugggcacaua ccaugaucuc     1800

aagaaaaucg uguccaagga ccugcuggau aaccccgaga acgaagacau ucuugagaau     1860

gucgugcuca cccugacgcu guuugaggau agagaaauga uccgcaaacg gcucgagaag     1920

uauaaggacg uccugacaga ggaacagcgg aaaaagcucg aacggcgcca cuacaccggc     1980

ugggggagac ugucugccaa acucauuaac ggaauccggg auaaggugac cagaaaaaca     2040

auucuggacu aucugaucga ugacggcacc agcaaucgca acuucaugca gcuuaucaau     2100

gaugacacac ucuccuucgu ugaugagauu cggcuggccc aagggagcgg agaggcugaa     2160

gacuacagag ccgaggugca gaaccuggcg ggcucuccgg cuaucaagaa ggggauucuc     2220

cagucccuga aaaucgucga cgaacuuauu gaggugaugg gcuaugaucc ugagcauauc     2280

guuguggaaa uggcccgcga gaaucaguuu acgaaccaag gaagacggaa uagccagcag     2340

cgcuacaaga aaaucgaaaa cgccauuaag aaucuggacu cuaaccucaa uuccaaaauc     2400

cugaaggagu accccaccaa caaucaggcu cugcaaaacg auagacuuuu ccucuauuac     2460

cugcagaacg gcaaagacau guauacagau gaggaacugg acauugauca gcucagccaa     2520

uacgacaucg aucacauuau cccucaggcc uuuaucaagg acgauucccu ggacaauaag     2580

guccuuacca aaucugcuaa gaaccggggg aaaagcgacg augugcccuc ccuggagauu     2640

gugcauaaga aaaagaauuu cuggaagcag cuccuggacu cucagcugau cagccaacgc     2700

aaauuugaua accuuacaaa ggccgaaaga ggaggccuga ccaaugagga caaagcgcgg     2760

uucauucagc gccagcucgu ugaaacgaga cagaucacca agcacgucgc ccggauucug     2820

gauacacgcu uuaacaccaa acucgacgag gcugggaaua gaauccggga ucccaaggug     2880

aacauuauca cacugaaguc caaucuugug agccaauuca gaaaagacua ucagcuguac     2940

aagguccgcg aaaucaacaa uuaucaccau gcccacgaug cuuaccucaa cgccgugguu     3000

gcgaccgccc ugcugaaaaa guacccucag cuugcuccgg aguuugugua uggagacuac     3060

cccaaauaua auucuuacaa guccagaaag agcgccacgg agaaaguccu guucuauucu     3120

aacauuauga auuucuucag acgcgugcuc guuuacucca agacaggcga agugcggauc     3180

agaccuguca uugaggugaa caaagaaacc ggggagaucg uuugggauaa gaaaagcgac     3240

uuccgcacag ugcggaaggu ccugucuuau ccccagguga acguggucaa gaaaguugaa     3300

augcaaaccg gcggauuuag caaggagucc auucuccagc auggcgauag cgacaaacug     3360

aucccuagaa agaccgagaa auucuaccuu gacacaaaga aauacggggg auuugauucu     3420

cccacgaucg cuuauuccgu gcugcucauu gccgacaucg aaaagggcaa ggccaaaaag     3480

cugaaacgcg ugaaggagcu gauugggauc accauuaugg aacggauggc uuucgagaaa     3540

aauccgaucg aguuucuuga acacaaggga uacaagaaca uccuggagaa aaauauuauc     3600

aagcucccua aauauagccu guucgaacuc gagaacggca gacggcgccu gcuugcgucc     3660

gccaaggaac ugcagaaagg gaaugagaug auucugcccc cucaccucgu cacacugcuu     3720

uaccauucua gcaacaucca caagauuacc gagcccaucc aucugaauua ugugaacaag     3780

aauaaacacg aauuuaagga gcuccugaga cauauuuccg auuucucuac acgguacauc     3840

cucgcucaag accgccugag caaaaucgag gaacuuuaug auaagaacga cggcgaugac     3900

auuuccgauc ugaccucuag cuuuguuaau cugcucacgu ucacagccau cggagccccu     3960

gcugccuuua aauuccuggg cuccgugauu gacagaaagc gguacaccag caucgcggaa     4020

auucuugagg cuacccugau ccaccagucu gucacagggc ucuacgagac cagaaucgau     4080

cuguccaagc ucggaggcga cuga                                            4104


<210>  4263
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4263
auggacaaga aauacagcau cggccuggau auugggaccu ccucuaucgg auucgugggc       60

gucgacgaga acggguggcc ccucaauauu aagggaaaaa gagccaucgg cgcucaccug      120

uuucgggaag gggaggccgc ggcugaaaca cgcggauuca gaacgacccg gcgcagauau      180

aaccggcgca aguggagacu ucggcugcuc cgcgagauuu uugaugaacc uaucagcaga      240

guugacccga cauucuuugc ccggaugaaa gaguccucug ugagccccaa ggauccuaau      300

uacgacggcc uggccaagga gauccuuauu ccccgcgaua aagacggcaa gauccuguau      360

ccuaccauuu accaucucag acacgcucug auguaugaag aucaucaguu cgacauccgg      420

gagauuuacc uggccaucca ccauauugug aaauaucgcg ggcacuuucu ccuggaugga      480

gcugccaaga acuucgaauc cucuaaucuu gaccugaaaa gcgauaucca cgagaucaac      540

gcgauucucg ucggcaucuu ugggaaugag caagugcagu ucaacgacca guacguugaa      600

aagcugaagg aggugcuucu ggacuccaca cagagcagau cugaucggca aaaagccgcu      660

gccaaggucc uccugcccac caaugaccug gauaaagaca caaccaagau ucucaaaacg      720

guguccaccg aaaucacaaa ggcuauuacc ggacugaaag ccaaguuuuu cguuaucaca      780

ggcaacgaua ucgcgaagga ccagcagaaa gauuggaccu uuagcauuga cacgcuugau      840

gagaagaugu cugugaucga accuuccuuc agcgagaaaa auuucucucu gauuacaauc      900

cuccagaagc uguacuccag cgucacccuu gccgggauug ugccgaacgg acugucccuc      960

ucugaggcua ugauccaaaa auaugacaga caugccagcg aucuggugcu gcucaagaag     1020

uacugggaca caaaacccaa gguccugaaa caccagcuuc agaauaccua ugcugaauac     1080

aucgagauuc aucagguugu ggacgaauuu uauuccgauc ugagcaagcu caucaaaaac     1140

gacgaugaca cccuggccca agagauuaag ucuaagauca caauuggcaa uuuccuuccu     1200

aaacagcgca cgaccgaaaa cggcgugauc cccuaccagc ugcaacagcg ggagcucgau     1260

acaauuauca agaaccagca ggaguauuac ccuuggcugg ccgaagagaa ucccgucgcu     1320

gaccaccaaa ucgcguuucc guacaaacug gaugaacucg ugggguuccg cauuccuuau     1380

uacguuggac ccaugaucgc caaggaggac accuccaaca gcaaaaauuc uaaguuugcu     1440

uggaugauua gaaagcagag cggcgaagug acaccuugga acuucgauga gaaaaucgac     1500

cggaccgccu ccgccacggc uuuuauugau agaaugacaa ccaccgacac auaucugauc     1560

ggggaggaug uccuucccaa gcagucucug aucuaccagc gcuucgaagu gcucaaugag     1620

cugaacaaaa uuagcaucga cggacaaccc auuacccgga agcagaaaca guccguuuau     1680

acacacgugu uugaaaagaa aaagacgguc caggugaagg acguccaaaa auuccuggug     1740

agcucuggcg agguuauguc ccuuccuaag aucgggggac ucgccaauga gaaacaguuu     1800

aacagcucuc uguccacccu guacgauuau aauaagauua ucccggacgc gcucgaagau     1860

agaaacaaag uggacgauau cgagaagauu auccugugga gcacaauuuu cgaagacaau     1920

uccaucuuug ucgagaaacu ugauucuauu gacuggcuga ccagcuccca gcgcaaacgg     1980

cucgcucaga agagauaccg cggcuggggc cggcugucuc ugagacuucu cacaaccuuc     2040

aaaaacaagg auggguacag cauccuggac gaacugugga acuccaauca uaauuucaug     2100

gagauccaag cccagagcga uuuuucugag cagauugcca cggcuaacaa caauaaccuc     2160

ucccaagugc ugcccgccaa augggaaccu ggacggaaga aucuugcuag ccuggacgag     2220

uucaucucua accucuauac cagcccccag aauaagaaag cccugcgcaa ggcgauuaga     2280

guugugcagg auaugaugac agccgcuggc cacaugccug aaaagaucuu uauugagguc     2340

gcccgggaca ccuccaacuc ucaucagcaa acaaucgcua gacagcgcca gcugcagcaa     2400

cuuuaccaga gcgaagccaa ggaggugauu gacuccagcg ugcggucuga gcucgaugac     2460

auccugaaaa auagagcgaa caucaaggau cagcuguucc ucuauuuuca gcaaggggga     2520

cgcgaccugu acaccggcaa ggccauuaau aucgauaaac uuucccugua ucacauugac     2580

cauauccucc cccagagccu gguuaaggau gacucccugg auaacagagu ccuugugucu     2640

cagaaaacga augaagacaa gggggugaca uucgucagcg auaaauacau gaacaauaug     2700

cucccgaccu ggaagcggug gcacgagaug ggccugauuu ccgcucgcaa acugaagaac     2760

cucacaauga gaccugacga aaucgacaag uaugcccccg gauuuaucca acggcagcug     2820

guggagaccc gccagguuac caaacuugug auugaacugc ucgaugcucu gcugggcaau     2880

ucucagacag agaucauuac gaucaaggcc agccuuuccc accaauucag acagcaguuu     2940

gaccugguca aaauucggga ggugaacgau aaccaucacg ccuucgacgc uuuucucgcg     3000

gcccgcaucg ggaccuaccu guacaagaga uauccuaaac uccggcccua cuucguuuau     3060

ggacaguaca ugcaaaauca uaagaaccug aagucuauca aucuuauuaa aacacuggau     3120

agcgacaagg cuaucgugga ugaaggcacc ggggagauuc ucugggacaa aaaguccgau     3180

cuggccaaaa ugaucaagau uuauaacuac cagcgccugc uuaucacaca cgaaagcaga     3240

accucuuuug gagccaugua uaagcagaaa auucugaagg cuacggacgc caaacagaau     3300

ggcgggggcu ucaagcucgu cccuacaaaa cggaacuggc ccguggauau cuacggaggc     3360

uacaccgggc aaaccgcggc uuaucugaca aucguuagag uguacuccaa gaagaccagc     3420

gagcuccgcg uccugccgau uuauacaaau aaagugcagc agaucgagaa ggccaugacg     3480

cagggagaca aagccaccau gcuugcuuuc aagaaaguga uugccccuca acugggcaag     3540

gaaaacaaga aaacacacga gaucaagauu cccgaauuug augucguuau ccccaaagug     3600

cuccuggacc agcuggugau cgacgauauu gcggggucuc cucaucgguu cagacuugga     3660

accgacacau acuaucacaa uucccagcaa cuguuucucc cgcugagcau gcagcgccag     3720

cucauggauc gggagucuga cgauaagguc uaccuggaca ucuucaacga ugugcuuaaa     3780

cagguugacu ccuauuuuaa ucuguacgau gugaacggcu ucagagcugc ccugcaaaag     3840

agcaaaaagg ccuuugaaca gcuccccauu aaugacuucc cuaaggaugu ccccgacaac     3900

aaaaagaaaa aggggaaacg cuccauccug aaggagauuu uuaucggccu ucaugcuaau     3960

gccacccugg gaaaccucaa gcagaugggc aucaaaacgg auuucgggaa gcugcagaaa     4020

agcaacggac uccggauuac cgagaauugc aagaucauuc accaaucucc uacaggccug     4080

uuugaaagaa ucgugcccau uaaauga                                         4107


<210>  4264
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4264
augagcagac ccuacaacau cggccuggac auugggaccu ccucuaucgg auggagcgug       60

gucgaugacc aguccaagcu cguuucugug cggggcaaau auggguacgg agugcgccug      120

uaugaugagg gccaaacagc cgcugaaaga cggagcuucc gcacgaccag acggcgccuu      180

aagagacgga aauggcgccu ggggcuccug agagagauuu uugaaccuua caucacaccg      240

gucgacgaua ccuucuuucu ucggcagaag caguccaauc ugucucccaa agaccagcgc      300

aagcucuauc cucaaacaag ccuguucaac gauagaaccg accgggccuu uuacgaugac      360

uaucccacaa uuuaccaucu gcgcuacaag cucaugaccg agaaaagaca guucgauauc      420

cgggaaaucu aucuggcgau gcaccacauu gugaaguaca gaggacauuu ucuuaaugag      480

gcuccuguuu ccagcuucaa aucuuccgag aucaaccugg uggcccacuu ugaccgccuc      540

aauacgauuu ucgccgaucu guuuagcgaa ucuggcuucc agcuugagac cgacaagcug      600

gcugaaguca aagcccuccu gcuggacaac cagcaauccg cuagcaaucg gcagagacag      660

gcccucucuc ugaucuauac acccuccacc aacaaggcgg uggagaagca gaauaaagcc      720

auugcuacag agcuucugaa ggccauccuc ggccugaaag cuaaguuuaa cguucuuacc      780

gggauugaag ccgaggaugu gaaagcgugg acgcugacau ucaaugccga aaacuucgac      840

gaggaaaugg ucaagcucga gagcucccug gaugacaaug cucaucaaau caucgagucu      900

cugcaggaac ucuacagcgg agugcugcuu gccggcauug ugccugagaa ccagucccug      960

agccaagcua ugaucaccaa auaugaugac caccagaagc accucaagau gcugaaagcc     1020

guccgcgaag cccuugcucc ggaggaucgg cagagacuga agcaggcgua cgaccaauau     1080

guugaugggc aggaaaauac aaaagccuac ucuaaggagg acuuuuacgg agauauuacc     1140

aaagcucuca agaacaaccc cgaccauccu aucguguccg agauuaagaa acugaucgaa     1200

cuggaccagu ucaugcccaa gcagcgcacc aaagauaaug gcgccauccc ucaccaacuc     1260

caucagcagg agcuggaccg gauuaucgaa aaccagcaac aguauuaccc cuggcuugcc     1320

gagcugaauc cgaacagcaa gagacagaca guggcuaaau auaagcucga ugaacugguc     1380

gccuuucgcg ugccuuacua uguugggccc cugauuacgg cggagcagca acggcagucu     1440

agcgacgcua aguucgccug gcuuaucaga aaagccgagg gacgcauuac cccuuggaau     1500

uuugaugaca agguggaucg gcaggcuucc gccaacgagu ucaucaaaag aaugacaacc     1560

acagacaccu accuccuggc ugaggauguc cugcccaagc aaucucucau cuaucagcgg     1620

uuugaagugc ugaaugagcu uaacggccug aaaauugacg aucagcccau cacgacagag     1680

cucaagcagg ccauuuucac cgaccuguuu augcaaaaga ucagcguuac cgugaaaaau     1740

auucaggauu accuggucuc cgaaaagcgc uacgcgagca gaccugccau cacaggccuu     1800

ucugacgaga acaaauucaa uucccggcuc agcaccuauc acgaccugaa gaugauugug     1860

ggggaugcug ucgacgaugu ggacaaacag gccgaucugg aaaagugcau cgaguggucu     1920

acaaucuuug aagacggaaa aauuuacucc gcuaagcuca acgagaucga uuggcugacg     1980

gaccagcaaa gaguucagcu ugccgcgaag cgcuaucggg gcugggggag acugagcgcc     2040

aaacuccuga cccagauugu gaaugcuaac ggacagcgca ucauggaucu gcuuugggac     2100

acaaccgaua auuucaugag aauuguccac uccgaggacu uugauaagcu caucacagaa     2160

gccaaccaaa ugaugcuggc ugagaacgac gugcaggacg uuaucaauga ucuguacacc     2220

ucuccgcaga acaaaaaggc ccuccggcaa auucugcuug uggucaauga cauccagaaa     2280

gccaugaagg gccaggcucc cgaacgcauu cugaucgagu ucgcgagaga agaugaggug     2340

aacccucggc ucagcgugca gcgcaagaga caaguugagc aggucuauca gaauauuucc     2400

aacgaacugc ugaauaacac ggagauccgg aaugaacuua aagaccucuc uaacagcgcc     2460

cuguccaaua cccgccuguu ccucuacuuu augcaagggg gaagagauau guauacaggc     2520

gacagccuga acaucgaucg gcuuucuacc uacgacauug aucauauccu gccccagucc     2580

uucgugaagg acaauagccu cgauaaccgc gugcuggucu cucagaaaau gaauagaucc     2640

aaggcugacc aggugccuac agacuuuacc agcguugagc ugggcaaaaa gaugcagcuu     2700

caaugggaac agaugcugcg ggccgggcuc auuacgaaga aaaaguacga uaaccugaca     2760

cucaaccccg accacaucuc uaaauaugcc augaagggau ucauuaauag acagcuggug     2820

gagacccgcc aggucaucaa acuugcuaca aaccugcuca uggagcaaua cggcgaagau     2880

aauaucgagc ugauuaccgu uaagagcggg cugacccauc agaugcggac agaauuugac     2940

uucccgaaga acagaaaucu uaacaaucac caucacgccu uugaugcgua ucugacggcu     3000

uucgugggac ucuaccugcu caaacgcuau ccuaagcuga aacccuacuu uguguauggc     3060

gaguaccaga aggccuccca acaggacaaa uggcggaacu ucaauuuucu uaacgggcug     3120

aagaaagaug agcucgucga cgaaaauacc gaggccguga ucugggauaa ggaaagcggc     3180

cuggcuuacc ugaacaagau uuaucaguuc aaaaagaucc uuguuacaag agaggugcac     3240

gaaaauucug gagcccuguu uaaccagacc cucuacgcgg cuaaagacga uaaggccucc     3300

ggccaagggg gaaaacagcu gauuccugcc aagcaggacc gccccacagc ucucuauggc     3360

ggguacagcg gaaagaccuc cgccuaucug ugucuuguca aaaucaagaa uaaaucugag     3420

uacauuuauc ggaucuucgg cgugaacacg agcugguccu cugagcugaa gcagauccaa     3480

gauccugcug aaagacuggu gcgccucgag aaacugcuua caccccagcu gaccauuaac     3540

aagaagaaaa agggggaacc ggucccuguu accaaagacu ucaagaucau ucucccccgg     3600

auccugauua aucagaaauu uuacagcaga ggccaggaca ucuccaucgc cucugaguuc     3660

uaccuccaua acgaacaaga gcuggugcuu gauaagagcg acauuaagau ccugaaugga     3720

gcgauuaaag ugaacaauuc caacaagcag gucaucaauc uguuuaacca cauucucgaa     3780

caggugaauc aauauuuccc cguuuacgau aucuauaaaa gccgggaagu gcugaacucu     3840

uccaaggaga ucuuugaaaa ucuuacaugg aacgaccagu ggcgcaauag aaaacugauu     3900

aacgucggcc agaagaccau ccucaaucgg auucugaucg ggcuccaugc caacgcugcc     3960

cgcauuaaga ucagcgagcu gggauucaaa aaugauuuug gccuucugac acagacgucu     4020

gggauuaccu ucacaccuga cacccaaauc aucuaccagu ccccgacagg acuguuugaa     4080

agacgggugg cucucagaga ucuguga                                         4107


<210>  4265
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4265
augaagagaa gcuacaacau cggccuggac auugggacca auuccaucgg augggccauu       60

guggaugacc agucuaaacu caucagcguc aagggcaaau auggguacgg aguucggcug      120

uaugaugagg gccaaacagc ugccgaacgc agauccuucc ggacgacccg cagacggcuu      180

aagcgcagaa aauggcggcu ggggcuccug cgcgagauuu uugaacccua caucacaccu      240

aucgacucua ccuucuuucu uagacagaag cagagcaacc uguccccgaa ggaucagcgg      300

aaacucuauc cccaaacauc ucuguucaau gaccgcccug augcggacuu uuaccagcag      360

uaucccacca uuuaccaccu gagauaccag cucaugacag agaagcggca auucgauauc      420

cgcgaaauuu aucuggcuau gcaucacauc gugaaauaca gaggacauuu ucuuaacgag      480

gccccugugg ccagcuucaa guccagcgag auuaaucugg cugaacacuu ugaccagcuc      540

aacaccaucu ucgccgaucu guuucgggag ucuggcuucc agcuugaaac ggacgagcug      600

gucgcuguga aagcccuccu gauggauaau cagcaauccg cgagcaacag acagcgcaag      660

gcccugccca agcucuauac cccuucugcu gacaaaucca uugagaagca gcggaaagcc      720

aucgcuacag aaauccugaa ggccauucuu ggccugaaag cgaaguuuaa uguugugacc      780

gggaucgagg ccgaagacgu caaggcuugg gcccucacau ucaacgcuga gaauuucgau      840

gaagaacugg ccaagcugga gagcucucug gacgauaacg gacaugaagu gauuggcauc      900

cuccaagagc uguacuccgg gauucugcuc gccggaaucg uuccggaaaa ucagagccug      960

ucccaggcua ugaucaccaa guaugaggaa caccaggcgc accuuaaaau gcugaaggcc     1020

gugagagacg aucucgcuac ggccgaccaa cagaaacuga agaaagccua cgaucaguau     1080

auugacggcc aggagaacac aaaggcuuac ucuaaggagg ccuuuuacga ugacgucacc     1140

aaagcgcuua aggauuauag cgaacaaccc cugguguccg agauccagaa acucauugaa     1200

cuggaccagu ucaugccuaa gcagcgcaca aaagacaaug gggcuauccc ccaucaacug     1260

caccagcagg agcucgaucg gauuaucgaa aaccagcaac aguacuaucc uuggcuggcc     1320

gagcuuaacc ccaauccgaa gagacagacc guggccaagu acaaacugga cgagcucguc     1380

gcuuuucgcg uuccuuauua cgugggaccc cugaucaccg ccgaacaaca gcagcagagc     1440

ucuaacgcua aguucgccug gaugauucgg aaaacagagg gcagaaucac gccuuggaau     1500

uuugaugaca agguggaucg ccaagcgucc gccaacgagu ucauuaaacg gaugaccaca     1560

accgacacau aucugcuugc ugaggauguc cuccccaagc agagccugau cuaccagaga     1620

uuugaggugc ugaaugaacu caacggccug aagauugacg aucagcccau caccaaugag     1680

cuuaaacaag ccauuuucaa cgaccuguuu augcagaaga cgucuguuag cgugaaaaau     1740

auccaggauu accucgucuc cgaaaagcgc uaugcuucuc ggccugccau cacagggcug     1800

agcgacgaga aaaaguucaa cuccagacug agcaccuacc augaucuuaa agugauuguu     1860

ggagacgcgg uggacgaugu cgacaagcag gccgaucucg aaaagugcau cgaguggucu     1920

accauuuuug aggacggcaa aaucuauucc gcuaagcuga augaaauuac auggcugacc     1980

gagcaacagc gggggcagcu cgccacaaaa cgcuacagag gauggggccg gcugagcgcu     2040

aaacuucuga cgcaaaucgu gaacgccaau gggcagagaa ucauggaucu ccugugggac     2100

accacagaua acuucaugcg cauuguccac uuugaagacu ucgauaaacu gaucaccgag     2160

gccaaucaga uggugcuugc ugaaaacgac guucaggaug ugauuaacga ccucuauaca     2220

ucuccgcaaa auaagaaggc gcugcggcag auccugcucg ucgugaacga uauucagaaa     2280

gccaugaagg gacaggcucc cgagagaauc cugaucgagu ucgcccgcgg cgcccaaaaa     2340

gacaaguccc uuaccguuag acggaaacgc aagcuggacg aaauguacag ccagauuucc     2400

gaugcugugc ucgccgacga gucugucaga caggaacuua agagcuccaa ucaggaugac     2460

cuggugaaag agaaggugua ucuuuacuuu augcaaggcg ggcgggaucu cuacacggga     2520

aacgcgcuga auaucgaccg ccugucugau uaucacguug accauauuau cccucaggcu     2580

uacauuaaag augacagccu cgauaacaag gcccuggucu ccagcagaau gaaugaaauc     2640

aaagccgacc gggugccccu ucaggugguc cagaagcaau ucggcaagaa cauggcuucu     2700

gagugggggu auaugcaccg cgccggacug aucuccagaa agaaguacga caaucucaug     2760

uuuaacccug agaaucugaa caaauaucag auuaaucggu ucaucagccg ccagcuggug     2820

gaaacccaac agguuauuaa acuuguggcg aaccugcucu cuaaucagua cgagggcgau     2880

aacgucaaaa ucauuacagu gaaguccaaa cugacccaga caaucagaag cgaauuugac     2940

uuccccaaga accgggaggu uaauaacuau caucacgcuu uugaugccua ccucaccgcc     3000

uucgugggga uguaccugcu uaagagauau ccgaaacugu cuagcuacuu uacguauggc     3060

gaguaccuca agggaaauca acaggacaaa ugguccagcu ucaacuuucu gaaugaucug     3120

aagcgcgcua aacuugaaga caagacaaac aaggagauuu ucuggcauaa agaaucuggc     3180

cuggccuauc ucaauaagau cuaccaguuu aaaaagaucc uggucacccg ggaggugcac     3240

gaaaacuccg gggcucucuu caaucagaca cuguaugccg cgaaagauga caaggccagc     3300

ggacaaggcg ggaagcagcu uauuccugcu aaacaggcca gacccaccgc ucuguacgga     3360

ggcuacuccg ggaagaccgu ugccuauaug uguaucgugc gcauuaaaaa caagaaaggc     3420

gaucucuaca aggucugcgg aguggagaca ucuuggcugg cccagcugaa gcaacuuaaa     3480

gacgaggaua gccagaaggc uuuccugaaa cagaagaucu ccccucaauu uacgaaagcg     3540

aagaagcaga aaggcaccau ugugaagguc guugaagacu ucgaggugau cgcuccccac     3600

auccucauua aucagcggcu guuugauaac gggcaggaac ucacacuggg aucugccacc     3660

uauaaacaua augagcaaga acuuauccug gacaagacag ccgugaaacu gcucaacggc     3720

gagcugccuc uugcccagag cgaggaacug gcugagcucg ucuacgacga aauucuggau     3780

caggugaugc acuauuuccc ccucuacgac accaaucagu uuagagccaa gcugcuugcg     3840

gggaaaacgg cuuucaugca acugccgugg aaguccaacc cugaugacaa caagacaguu     3900

cagaucaccc agcccauugu gcugaaaaag auccuccagg gacugcaugc cgaugccggc     3960

cgcgucucuc uuaaagagcu ggggaugacc acagacuuug gcaugcucca aaaggcuacc     4020

ggaaucacau ucacgcccga uacccagauu aucuaucaga gcccuacagg ccuguuugaa     4080

cggagagugg cccuccgcga ccuguga                                         4107


<210>  4266
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4266
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcugggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug auuccggaga gaccgccgaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuugc      240

uaucugcagg agaucuucuc uaacgaaaug gccaaagugg acgauagcuu uuuccauaga      300

cuugaggaau ccuuucuggu cgaggaggac aagaaacacg aacggcaucc uaucuucggc      360

aauauugugg augagguugc cuaccacgaa aaguauccga ccaucuacca ucuccgcaag      420

aaacuggugg acucuacaga uaaggcugac cugagacuca uuuaucuggc ccuugcucac      480

augaucaaau uucgggggca cuuccugauu gagggagauc ucaaccccga caauagcgac      540

gucgauaagc uguuuaucca acuugugcag accuacaacc agcuguucga ggaaaauccu      600

auuaacgccu ccggcguuga cgcgaaagcc auccucagcg cucgccuguc uaaguccaga      660

cggcuggaga aucucaucgc ccagcugccc ggcgaaaaga agaacgggcu uuuuggaaau      720

cugauugcuc ucagccuggg ccuuacgccu aacuucaagu cuaauuuuga ucuggccgag      780

gacgcgaaac uccaacuguc caaggauacc uacgacgaug accuggauaa ccuccuggcc      840

cagaucgggg accaguaugc ugaucuuuuc cuggccgcua aaaaccucag cgacgccauu      900

cugcuuucug acauccugcg cgugaauaca gaaauuacca aggcuccccu cuccgcuagc      960

augaucaaaa gauacgauga gcaucaccag gaccugacac ugcucaaggc gcugguccgg     1020

caacagcuuc cugagaagua uaaagaaauc uucuuugauc aguccaagaa cggauacgcc     1080

ggcuauauug acgggggagc uucucaggag gaauuuuaca aauucaucaa gccgauucug     1140

gagaaaaugg auggcaccga ggaacuccug gugaagcuga auagagagga ccuucuccgc     1200

aagcaacgga cguucgauaa cggcagcauc ccccaucaga uucaccuggg ggaacugcac     1260

gccauccuca gacgccagga ggacuuuuau ccuuuccuga aagauaaucg ggaaaagauc     1320

gagaaaauuc uuacauuuag aauccccuac uacgugggac cucuggcccg cggcaacucc     1380

cgguucgcuu ggaugaccag aaagagcgaa gagacaauua cccccuggaa uuuugaggaa     1440

gucguugaca aaggggccuc ugcgcaaucc uucaucgagc gcaugaccaa cuuugauaag     1500

aaucucccga acgaaaaggu gcugccuaaa cauagccugc uuuaugagua cuucacagug     1560

uauaaugagc ucacgaaggu caaauacgug accgaaggaa ugcggaagcc cgcuuuucug     1620

ucuggcgagc agaaaaaggc cauuguugac cuucucuuca aaacaaacag aaaggugacc     1680

gucaagcagc ugaaagaaga uuauuucaag aaaaucgagu guuuugacag cguggaaauu     1740

uccgggguug aggaccgcuu caaugccucu cuuggaacau accacgaucu gcucaagauc     1800

aucaaagaca aggauuuucu ggacaacgag gaaaaugagg auauucugga agacaucgug     1860

cuuacccuca cgcuguucga ggaucgggaa augauugagg agagacugaa gacauaugcu     1920

caucucuuug acgauaaagu caugaagcag cugaaacggc gcagauacac cggcuggggc     1980

cggcuuagca gaaagcugau caacgggauu cgcgacaaac aauccggaaa gaccauccuc     2040

gauuuccuua agagcgacgg cuuugccaac cggaauuuca ugcagcugau ccacgaugac     2100

ucucugacau uuaaagaaga cauucagaag gcucaggugu ccgggcaagg agauagccuc     2160

caugagcaca ucgccaaccu ggcgggcucu ccugccauua aaaaggggau ccugcagacc     2220

gucaaagugg uugacgaacu cgugaagguc augggcagac acaagcccga gaauauugug     2280

aucgaaaugg cucgcgagaa ccagacaacg cagaaaggac aaaagaacuc cagagagcgg     2340

augaaacgca ucgaagaggg cauuaaggaa cuggggagcc agauccuuaa agagcauccc     2400

guugagaaua cccagcugca gaaugaaaag cucuaccugu auuaccugca aaacggaaga     2460

gauauguaug uggaccagga gcuugauauu aaucggcugu ccgacuacga ugucgaccac     2520

aucgugccuc agucuuuccu caaagaugac agcauugaua acaaggugcu gacacgcucc     2580

gacaagaaua gaggcaaauc ugacaacguu ccgagcgaag aggucgugaa gaaaaugaag     2640

aauuauuggc ggcaacuccu gaacgccaaa cuuaucaccc agcgcaaguu ugauaaucug     2700

acaaaggcug aaagaggggg acucuccgag cuggacaaag ccggcuucau caagcggcag     2760

cugguggaga cccgccagau uacgaaacau gucgcgcaaa uccuugauag cagaaugaac     2820

accaaguacg acgaaaacga uaaacugauu cgggagguga agguuaucac acucaagucu     2880

aaacuggugu ccgacuuuag aaaggauuuc caguucuaua aaguccgcga aauuaauaac     2940

uaccaccacg cccaugacgc uuaccucaau gccgugguug ggaccgcucu gaucaagaaa     3000

uaucccaagc uggagagcga auuuguguac ggcgauuaua aggucuacga cgugcggaaa     3060

augauugcca agucugagca ggagaucgga aaagccacag cuaaguauuu cuuuuacucc     3120

aacaucauga auuucuuuaa aaccgaaauu acgcuggcga acggcgagau cagaaagcgc     3180

ccucucauug aaacaaaugg ggagaccgga gaaaucguuu gggauaaggg ccgggacuuc     3240

gccacaguga gaaaaguccu gagcaugccc caggugaaca uugugaagaa aaccgagguc     3300

caaaccgggg gauuuucuaa ggagagcauc cugccuaaac gcaauuccga uaaacuuauc     3360

gcucggaaaa aggacuggga ccccaagaaa uauggcgggu ucgauagccc gacaguugcc     3420

uacucugugc ugguggucgc caagguggaa aaaggcaagu ccaaaaagcu caagagcguu     3480

aaagagcugc ucggaauuac gaucauggaa agauccucuu uugagaagaa cccuauugac     3540

uuccuggagg cuaaaggcua caaggaagug aaaaaggauc uuaucauuaa gcugcccaaa     3600

uauagccugu uugagcucga aaaugggcgc aagcggaugc uggccuccgc gggagagcuu     3660

cagaaaggca acgaacuggc ucucccuucu aaguacguca auuuccugua ucucgccagc     3720

cacuacgaga aacugaaggg gucccccgag gacaacgaac agaagcagcu uuucguggag     3780

caacauaaac acuaucugga ugaaaucauc gagcagauuu cugaauuuag caagagaguu     3840

auccuggccg acgcuaaucu cgauaaagug cuguccgccu acaacaagca ucgggacaaa     3900

ccuauucgcg agcaggcgga gaacaucauu caccuuuuca cccugacaaa ucucggagcu     3960

cccgccgccu uuaaguauuu cgauaccaca aucgacagaa aacgguacac cagcacgaag     4020

gaaguccugg augcuacacu cauucaccaa ucuaucaccg gccuguacga gacccgcauc     4080

gaccuuuccc agcucggggg cgauuga                                         4107


<210>  4267
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4267
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aagcucaaag gccuggggaa uacggacaga      120

cacggaauua agaagaaccu uaucggcgcu cuccuguucg auagcgggga gaccgccgaa      180

gcgacacggc ugaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuugc      240

uaucuucagg agaucuuuuc caacgaaaug gccaaagugg acgauucuuu cuuucauaga      300

cuggaggaau ccuuccucgu ggaggaggac aagaaacacg aacggcaucc uaucuuugga      360

aauauugucg augagguggc cuaccacgaa aaguauccga ccaucuacca ucugcgcaag      420

aaacuggcug acuccacaga uaagguugac cucagacuga uuuaucuugc ccuggcucac      480

augaucaaau uccggggcca cuuucucauu gagggcgauc ugaaccccga caauucugac      540

guggauaaac uuuucaucca acugguccag accuacaacc agcucuuuga ggaaaauccu      600

auuaacgcca gccgcgugga cgcgaaagcc auccuguccg cuagacugag caagucucgg      660

cgccucgaga aucugaucgc ccagcuuccc ggggaaaaga agaacggacu guucggcaau      720

cucauugcuc ugucccuugg gcugacgccu aacuuuaaga gcaauuucga ucucgccgag      780

gacgcgaaac ugcaacuguc uaaggauacc uacgacgaug accucgauaa ccugcuugcc      840

cagaucggag accaguaugc ugaucuguuu cucgccgcua aaaaccuguc cgacgccaca      900

cuucugagcg acauucucag aguuaauucu gaaaucacca aggcuccccu guccgccagc      960

augauuaaac gguacgauga gcaucaccag gaccugacac uccugaaggc gcuugugaga     1020

caacagcugc cugagaagua uaaagaaauc uucuucgauc aguccaagaa cggcuacgcc     1080

ggguauaucg acggaggcgc uucucaggag gaauuuuaca aauucauuaa gccgauccuc     1140

gagaaaaugg auggcaccga ggaacugcug gccaaacuua aucgcgagga ccuccugcgg     1200

aagcaaagaa cguuugauaa cgggagcauu cccuaucaga uccaucuggg agaacuccac     1260

gccauucugc gccggcagga ggacuucuac ccuuuucuua aggauaauag agaaaagauc     1320

gagaaaaucc ugacauuccg cauucccuac uaugucggcc cucucgcucg ggggaacucc     1380

agauuugccu ggaugacccg caagagcgaa gagacaauca cccccuggaa uuucgaggaa     1440

gugguggaca aaggagcguc ugcucaaucc uuuauugagc ggaugaccaa cuucgauaag     1500

aaucugccga acgaaaaggu ccugccuaaa cacagccuuc ucuacgagua uuuuacaguu     1560

uacaaugagc ugacgaaggu gaaauaugug accgaaggca ugagaaagcc cgccuuccug     1620

ucuggggagc agaaaaaggc caucgucgac cuccuguuua aaacaaaccg caaggugacc     1680

guuaagcagc uuaaagaaga uuacuucaag aaaauugagu guuucgacag cguggaaauc     1740

uccggagucg aggaccgguu uaaugcuucu cugggcacau aucaugaucu ccugaagauu     1800

aucaaagaca aggauuuccu ggacaacgag gaaaaugagg auauccuuga agacauugug     1860

cucacccuga cgcuguuuga ggauagagaa augaucgagg agcggcucaa gacauacgcc     1920

caccuguucg acgauaaagu uaugaagcag cuuaaacgca gacgguacac cggcuggggg     1980

agacugagcc gcaagcucau uaacggaauc cgggacaaac aauccggcaa gaccauucug     2040

gauuuucuua agagcgacgg guucgcuaac agaaauuuua ugcagcugau ccaugaugac     2100

ucucucacau ucaaagaaga cauccagaag gcccaggugu ccggacaagg cgauagccug     2160

cacgagcaua uugcgaaccu ggccgggucu ccugcuauca aaaagggcau ucuccagacc     2220

gucaaagugg ucgacgaacu ggugaagguu augggacgcc acaagcccga gaauaucgug     2280

auugaaaugg ccagagagaa ccagacaacg cagaaaggcc aaaagaauag ccgggagcgc     2340

augaaaagaa ucgaagaggg gaucaaggaa cuuggaagcg auauucugaa agaguauccc     2400

gucgagaaca cccagcucca gaaugaaaag cuguaccugu auuaccuuca gaacggccgg     2460

gacauguaug uggaucaaga gcuggacauc aaucgccucu ccgauuacga cguugaucac     2520

auugugccuc agucuuuucu gaaagacgau agcaucgaca acaagguccu cacaagaucc     2580

gacaagaauc gggggaaauc ugauaacgug ccgagcgaag aggugguuaa gaaaaugaag     2640

aauuauuggc gccagcugcu uaacgcuaaa cugauuaccc aaagaaaguu cgacaaucuc     2700

acaaaggccg aacggggagg ccuguccgag cuggauaaag ucggguuuau caagcgccag     2760

cuuguggaga ccagacagau cacgaaacau guggcgcaga uucuggacag ccggaugaac     2820

accaaguacg augaaaacga caaacucauc agagaggucc gcgugauuac acugaagucu     2880

aagcucguuu ccgauuuccg gaaagacuuu caauucuaca aggugagaga aaucaauaac     2940

uaucaccaug cccacgaugc uuaccugaau gccgucgugg gcaccgcucu uauuaaaaag     3000

uaucccaaac uggagagcga guucguuuac ggagacuaua agguguacga uguccgcaag     3060

augaucgcca aaucugaaca ggagauuggc aaggccacag cuaaauacuu uuucuauucc     3120

aacaucauga acuuuuucaa gaccgaaauc acgcucgcga auggggagau ucggaaaaga     3180

ccucugaucg aaacaaaugg agagaccggc gaaauugugu gggacaaggg gcgcgauuuu     3240

gccacaguuc ggaaggugcu gagcaugccc caggucaaca ucgugaaaaa gaccgaggug     3300

cagaccggag gcuucucuaa agagagcauu cuuccuaaga gaaauuccga caaacugauc     3360

gcucgcaaga aagacuggga ucccaagaag uacgggggcu uugacagccc gacagucgcc     3420

uauucuguuc ucgugguggc caaagucgaa aagggaaaau ccaagaaacu caagagcgug     3480

aaggagcugc ugggcaucac gauuauggaa cgguccucuu ucgagaaaga uccuaucgac     3540

uuucuugagg cuaaggggua caaagaaguu agaaaggauc ugauuaucaa acugcccaag     3600

uauagccucu ucgagcugga aaacggacgg aagcgcaugc uugccuccgc gggcgagcug     3660

caaaaaggga augaacucgc ucugccuucu aaguacguga acuuucucua ucuggccagc     3720

cacuacgaga aacuuaaggg aucccccgag gacaaugaac agaaacagcu guucgucgag     3780

cagcauaagc acuaucugga ugaaauuauc gagcaaaucu cugaguucag caagagagug     3840

auucucgccg acgcuaaccu ggauaaaguu cuuuccgccu acaauaagca ucgggacaaa     3900

ccuauccgcg aacaggcgga gaacauuauc caccuguuua cccucacaaa ccugggcgcu     3960

cccgccgccu ucaaguacuu ugauaccaca auugacagaa aacgguauac cagcacgaag     4020

gaagugcucg augcuacacu gauccaucag ucuauuaccg ggcuuuacga gaccagaauc     4080

gaccuguccc aacugggcgg agacuga                                         4107


<210>  4268
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4268
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcugggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug auuccggaga gaccgcugaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuugc      240

uaucugcagg agaucuucuc uaacgaaaug gccaaagugg acgauagcuu uuuccauaga      300

cuugaggaau ccuuucuggu cgaggaggac aagaaacacg aacggcaucc uaucuucggc      360

aauauugugg augagguugc cuaccacgaa aaguauccga ccaucuacca ucuccgcaag      420

aaacuggcug acucuacaga uaaggccgac cugagacuca uuuaucuggc ucuugcccac      480

augaucaaau uucgggggca cuuccugauu gagggagauc ucaaccccga caauagcgac      540

guggauaagc uguuuaucca acuuguccag accuacaacc agcuguucga ggaaaauccu      600

auuaacgcgu cccgcgugga cgccaaagcu auccucagcg ccagacuguc uaagucccgg      660

cgccuggaga aucucaucgc ucagcugccc ggcgaaaaga gaaacggccu uuuugggaau      720

cugauugccc ucagccuggg acuuacgccu aacuucaagu cuaauuuuga ucuggcggag      780

gacgccaagc uccaacuguc caaagauacc uacgacgaug accuggauaa ccuccuggcu      840

cagaucggcg accaguaugc cgaucuuuuc cuggcugcca agaaccucag cgacgccauu      900

cugcuuucug acauccugcg gguuaauucc gaaauuacaa aagcuccccu cagcgcgucc      960

augaucaaga gauacgauga gcaucaccag gaccugaccc ugcucaaagc ccuggugcgc     1020

caacagcuuc cugagaagua uaaggaaauc uucuuugauc agucuaaaaa cggguacgcu     1080

ggauauauug acggcggggc cagccaggag gaguucuaca aguuuaucaa accgauucug     1140

gaaaagaugg auggaacaga ggaacuccug gccaaacuga aucgggagga ccuucucaga     1200

aagcaacgca ccuucgauaa cggcuccauc ccccaucaga uucaccuggg cgaacugcac     1260

gcuauccucc ggagacagga ggacuuuuau ccuuuccuga aggauaaucg cgagaaaauc     1320

gaaaagauuc uuacguuucg gauccccuac uacgucgggc cucuggccag aggaaacagc     1380

cgcuucgcgu ggaugacacg gaaaucugaa gagaccauua cacccuggaa uuuugaggaa     1440

gugguggaca agggcgcuuc cgcccagagc uucaucgaga gaaugaccaa cuuugauaaa     1500

aaucucccga acgaaaaggu ccugccuaag cauucucugc uuuaugagua cuucaccguu     1560

uauaaugagc ucacaaaagu gaaguacgug acggaaggga ugcgcaaacc cgaguuucug     1620

agcggagaac aaaagaaagc cauugucgac cuccuguuca agaccaaccg gaaagugaca     1680

guuaagcagc ugaaggagga uuauuucaaa aagaucgaau guuuugacuc cguggagauu     1740

ucuggcgucg aggacagauu caaugcuagc cuugggaccu accacgaucu gcucaaaauc     1800

aucaaggaca aagauuuucu ggacaacgaa gagaaugaag auauucugga ggacaucgug     1860

cuuacacuca cccuguucga agaucgggag augauugagg aacgccugaa gacguaugcc     1920

caucucuuug acgauaaggu uaugaaacag cugaagagac ggagauacac aggauggggc     1980

cgccuuuccc ggaaacugau caacggcauu agagacaagc aaagcgggaa aaccauccuc     2040

gauuuccuga agucugacgg auuugcuaac cgcaauuuca ugcagcugau ccacgaugac     2100

ucccuuaccu uuaaggagga cauucagaaa gcccagguga gcggccaagg ggauucucuc     2160

caugaacaca ucgcgaaccu ggccggaucc ccugcuauua agaaaggcau ccugcagaca     2220

gucaaggugg ucgacgagcu cgugaaaguu auggggagac acaagcccga aaauauugug     2280

aucgagaugg cccgggagaa ccagaccaca cagaagggcc agaaaaauag ccgcgaaaga     2340

augaagcgga ucgaggaagg aauuaaagag cugggcuccg auauccuuaa ggaguacccc     2400

gucgaaaaca cgcaacugca gaaugagaaa cucuaucugu acuaucugca gaacgggcgc     2460

gacauguacg uggaucaaga acuugacauu aauagacugu cugauuauga cguugaucau     2520

aucgugccuc agagcuucau uaaggacgau uccaucgaca acaaaguccu cacccggucu     2580

gacaagaauc gcggaaagag cgauaacgug ccguccgagg aagugguuaa aaagaugaaa     2640

aauuacugga agcagcugcu caacgcuaaa cugaucacac aaagaaaguu ugacaaucuu     2700

accaaggccg agcggggcgg gcugagcgag cucgauaaag cgggauucau uaagcgccag     2760

cuggucgaaa caagacagau caccaaacac guggcccaga uucuggacuc ucggaugaac     2820

acgaaguaug augagaacga caaacuuauc agagaaguga aggucauuac ccugaagucc     2880

aaacucguga gcgauuuucg caaggacuuc caauucuaca aaguucggga gaucaauaac     2940

uaccaucacg cucacgaugc cuaucugaau gcuguggucg gcacagcccu cauuaagaaa     3000

uaccccaagc uggaaucuga guuuguguau ggggacuaca agguuuauga ugugagaaaa     3060

augaucgcca aguccgagca ggaaaucggc aaagcuaccg cgaaguacuu cuuuuauagc     3120

aacauuauga auuucuuuaa aacagagauc acccuugcca acggagaaau ucgcaagcgg     3180

ccucugaucg agacgaaugg cgaaacaggg gagauugucu gggacaaggg aagagauuuc     3240

gcuaccgugc gcaaaguucu cucuaugccc caggugaaca ucgucaagaa aacagaggug     3300

cagaccggcg gguuuagcaa ggaauccauc cugccuaaac ggaauagcga caagcugauu     3360

gcccgcaaaa aggacuggga ucccaagaaa uacggaggcu ucgacucucc gaccguggcc     3420

uacuccgucc uuguuguggc uaagguggag aaaggcaaga gcaaaaagcu gaaguccguc     3480

aaagaacucc uggggaucac aauuauggag agaucuagcu uugagaagaa cccuaucgau     3540

uuccucgaag ccaaaggaua uaaggaggug cggaaagacc ugauuaucaa acuucccaag     3600

uacucccugu uugaacugga gaauggcaga aaacggaugc ucgcgucugc uggggaacug     3660

caaaagggaa acgagcuugc ccugccuagc aaauauguua auuuccucua ccuggccucc     3720

cauuaugaga agcucaaagg cucucccgaa gauaacgagc agaagcagcu guucguggaa     3780

cagcacaagc auuaccuuga cgagaucauu gaacaaauca gcgaguuuuc caaacgcguc     3840

auucuggcug augccaaucu ggacaaggug cucagcgcgu auaacaaaca cagagauaag     3900

ccuauccggg agcaggcuga aaacauuauc caucuguuca cgcuuaccaa uuuuggggcc     3960

cccgccgcuu ucauuuacuu ugacacaacc aucggacgca acagauacaa aucuaucaag     4020

gagguucugg augccacacu cauucaccag uccaucaccg gccuguauga aacgcggauu     4080

gaccucagcc aacucggggg cgauuga                                         4107


<210>  4269
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4269
augaaguacg agagcgaaga guauuacauc ggccuggaca uggggaccuc cucuguggga       60

ugggccguca cagaucccca cuaucaucuc auuagaaaga agggcaaaga ccugugggga      120

gcucggcuuu ucgaaaaggc caacacggcg caggagcgca gaaccaaucg gaucaaccgc      180

agacggaauc aacgcagaaa acagcggauu gcuuuuaucc gcgaagccuu cagaaaggag      240

guugagaagg uggaugugaa cuuuuuccgg cgccuggacg aaagccagcu cuaccuggag      300

gauaaaucca uuaaggaacc uuaucuucug uuuaaugacc ucaacuacac agauaaagac      360

uucuaucacc aguacccgac cgucuuucau cugaucacag aucugcucac cucugacaga      420

aaguucgaua uccgggagau uuacaucgcc gugagcuccc uguauaauca ccgcgggaac      480

uuucuuaaua aaucucuggg caacgagaca caagacgaag aucucguuuu caagaaucug      540

gacgaggcuu uuagcgccug cgggaugaac uggaauacca aggcugaaaa cauugaggug      600

gucaaacaga uccuuuguga cagaucccug aauaagaaag aaaagcagga ucagcucgcc      660

cauugccugg agauuaacaa aaagacggag aaagaaaaga ucgaguuccu gaagcuccug      720

accggaggca aagcggacag caagaaaaug uuuccccaau accacuauga acgggaugag      780

aaacuuucuu ucgccuucgc ugacgaaaac ggcgaugaga auugggagga aacacugaac      840

gccauugagg aaagacucac cuccgacgag uacgggcuga uccuugcucu gaaaagcgug      900

uaugauuggu cuauccucgc cgaccugaug aagaaugaaa agacaggaga gaccuacccu      960

uaucugucca uggcgcgcaa agauagcuac gagaagcaca aagccgaccu ccggcugcuu     1020

aagucuguua uuaaaagacu gauccccaac gaauacaauc auuucuuccg ccaguaugau     1080

ggcaagggga agacguacac aaacuauauu ggauccgugc aguaccacga gaaacggcau     1140

agauuuaagc acaucaccac aaaagaauuu uaugacggcc ucaagaaacu gcuuaagccu     1200

uacagcgagg acgaggaaau uaagcagauc cugcaagaua ucgaguccga aaccuuucuc     1260

cccaaacaga uuaccaauau gaacgggguc aucccuaauc aggugcacgc uaaggagcug     1320

aaagugauuc uggaaaaggc cucucaacau uaugacuucc ucaacgagac aaaugagagc     1380

ggacugacgg ucaaagaaca gaucaugcag auguuuaccu uccagauucc guacuacguu     1440

ggcccccuug cuaaccugaa uggcaagggg aacggauggg ccgugcgcua ugugggcaaa     1500

gagcggacaa aggucuaccc uuggaauaug gaugaaguga ucgacgagaa ggaaaccgcc     1560

gaucaauuua ucaaaagaau gauuuccaca uguaccuauc ucggggacga guacguucug     1620

cccaagagcu cuauccugua ugagaaauuc gcugugcuua acgaacucaa cucccugaag     1680

auuaauggac agccugucag cguggaucug aaacagaaga ucuaccgcga ccucuuuacg     1740

aagggcaaac ggguuacaca gaaggggcug cuucuguauc ucaaaagaga gggagugauu     1800

ucuaagagcg aagagaccau ccuguccggc uucgauauug acuuuaaaaa cacccugucu     1860

agcuaccgca aguucugcga agucuucgcg acaaaugagc uuaacgauga gcaaauccgg     1920

auggccgaag acaucauuga guggcucacc guguuuggcg auuccaagaa auucgucaga     1980

gaaaagaucc acgggaauua cccccagauu acagcuaacc agcugaaacg gauccugagc     2040

uuuaauuuca aggacugggg aaaacucucu aagcaguuuc uggagcuuga gggcgaagac     2100

cgcgagacgg ggaaguccug uagccugauu caagcccucu gggauaccaa caauaaccug     2160

aaucagcugc uuucuuccag auucacauau aaagaaaacc ucgagaagaa acggaagaaa     2220

aucgaaaugg accuguuuga guucacccau gaggaucugg acgaucucua caucagcgcc     2280

ccggugaaga gaaugauuug gcaggcucug aaaaucauga aggacauugu uaucgugaug     2340

aaggaauccc cuacaaaacu uuuuauugag augacccgca auaacgaaaa ugagaagacg     2400

cggaccgucu cuagaaagaa caagcuggug gaucucuaua aaaagaucaa gggagaaagc     2460

cgcgacugga ucaaagagcu ggagagauac gaagagaagg auuuccgguc caaaaagcug     2520

uaucuuuacu auacacagau gggcaaaucu gccuacagcg gggaacccau ugacauggau     2580

aagcucuuug acaagaacgu uuacgauauc gaccacauuu auccuaaauc caaggugaaa     2640

gacgauagcc ugcugaauaa cugcguccuc gugaagcgca aagagaauau ggacaagggc     2700

aacaauuucc ccaucgaucc cgacauucaa accaagcagg aacaucugug gaaauuuugg     2760

cuugagaagg gacugaucac acaggagaaa uucaacagac ucacccggaa gaaaccuuuc     2820

acgaaugaug aacuggcgga cuuugugaac cgccaacugg uugagacagg ccaggcuacc     2880

aagacaaucg ccgaucuuuu caagaugacc uuucccaauu cugacauugu cuacgugaaa     2940

gccgggaacg ugaccgauuu cagacaguau aucgaagucu acaagugucg ggaggugaau     3000

aaccugcacc acgcuuauga cgccuaccuc aauguugugg ucggaaacgu guaugauaca     3060

aaauuuacga agaaucccau gaacuacauu aaagagcagc gcaaggaaaa cuccaaugag     3120

agauaccaua uggacaaaau cuucgaauuu ccuguuauuc ggaacggcaa ggaggcuugg     3180

aucaccaaga aagacgaaag cauuaauauc gugcgcaaga cacuggccag aaaaaccccg     3240

cucgucacaa agcggaacuu cgaggcgaau gggaccaucg ccaaagcuac gauuuauccu     3300

aagggaaacg ugaaggauca aggcuaccug cccagaaaaa caucuauccc ggcccuucgc     3360

gacaugaaga aauauggggg cguuaccucc auucagaucg cuuacuuuuu ccugguggag     3420

cacgaaguca agaaaaagcg gauuagaacc aucgagugcg ugccuaucua ucgcaaggag     3480

gaacucaaaa caaaggaaca gcuggagcgg uacuguagag aggaacugaa acuuauuaau     3540

cccagcaucc gccuggagaa gauuaaaccu uauucucucc ugaagaugaa cggaaacuuu     3600

uucuacauca ccggcaagac aaaugaucgg uucagccucg ccaaugcgau ggaacuguac     3660

guggacaaag ucuccgagaa ccauauuaga gaucuuguua aguauaaaaa uugcggguac     3720

uuuaacgugg acgauaagga agcccgcaaa cggaaugaga gacuguauac gauccucacc     3780

aagaaacacc ugacaguguu caagggaagc cccaagccua ugggcgagaa acugcagcaa     3840

uaccagcagc uuuuucugga acucgagguc ucuaaacagg uguauguucu gcuccaaauc     3900

cugcagcuua ccgcuacaaa cgugaaaacc aaggccgacc ugacggcuau ugggggaaaa     3960

gccaaugaag gcaccaugga uaucuccaag aaccuggaga acgucauuaa gaaucagauc     4020

aaaaaggggc ucgugcccga gcugaugcuu auuaaccaaa gcccgacagg ccuguucgaa     4080

uccgagaucg accuccugac cauuuga                                         4107


<210>  4270
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4270
augagcagac ccuacaacau cggccuggac auugggaccu ccucuaucgg auggagcgug       60

gucgaugacc aguccaagcu cguuucugug cggggcaaau auggguacgg agugcgccug      120

uaugaugagg gccaaacagc cgcugaaaga cggagcuucc gcacgaccag acggcgccuu      180

aagagacgga aauggcgccu ggggcuccug agagagauuu uugaaccuua caucacaccg      240

gucgacgaua ccuucuuucu ucggaagaaa caguccaauc ugucucccaa ggaccagcgc      300

aagcucuauc cucagacaag ccuguucaac gauagaaccg accgggccuu uuacgaugac      360

uaucccacaa uuuaccaucu gcgcuacaaa cucaugaccg agaagagaca auucgauauc      420

cgggaaaucu aucuggcgau gcaccacauu gugaaauaca gaggacauuu ucuuaaugag      480

gcuccuguuu ccagcuucaa gucuuccgag aucaaccugg uggcccacuu ugaccgccuc      540

aauacgauuu ucgccgaucu guuuagcgaa ucuggcuucc agcuugagac cgacaaacug      600

gcugaaguca aggcccuccu gcuggacaac caucaguccg cuagcaaucg gcagagacaa      660

gcccuccugc uuaucuauac acccucuacc aacaaggcgg uggagaaaca gaauaaggcc      720

auugcuacag agcugcucaa agccauccug ggccuuaagg cuaaauuuaa cguucugacc      780

gggauugaag ccgaggaugu gaagacgugg acacucaccu ucaaugcgga aaacuucgac      840

gaggaaaugg ucaaacugga guccagccug gaugacaaug cccaccagau caucgagucc      900

cuccaggaac uguacucugg agugcuucug gcuggcauug ugccugagaa ccaaagccuc      960

ucccaggcca ugaucacaaa guaugaugac caccagaagc aucugaaaau gcuuaaggcu     1020

guccgcgaag cccuggcccc ggaggaucgg caaagacuca aacaggcuua cgaccaguau     1080

guugaugggc aggaaaauac caaggcguac agcaaagagg acuuuuacgg agauauuacc     1140

aaggcccuga agaacaaccc cgaccacccu aucgugucug agauuaaaaa gcugaucgaa     1200

cucgaccaau ucaugcccaa acagcgcaca aaggauaaug gcgcuauccc ucaucagcug     1260

caccagcaag agcuugaccg gauuaucgaa aaccagcagc aguauuaccc cuggcuggcc     1320

gagcucaauc cgaacuccaa aagacaaacg guggccaagu auaagcugga ugaacugguc     1380

gcuuuucgcg ugccuuacua uguugggccc cuuauuaccg ccgagcagca gcagcaaagc     1440

ucugacgcga aauucgcuug gaugauccgg aaggccgagg gacagauuac accuuggaau     1500

uuugaugaca aaguggauag acaggccagc gcuaacgagu ucaucaagcg caugaccaca     1560

accgacacgu accuccuggc cgaagauguc cugcccaaac aaucccucau cuaucagcgg     1620

uuugaagugc ugaaugagcu uaacggccug aagauugacg aucagcccau cacaaccgag     1680

cucaagcagg cuauuuucac cgaccuguuu augcagaaaa caucuguuac cgugaagaau     1740

auccaggauu accuggucag cgaaaagaga uacgccuccc ggccugcgau uacaggccuu     1800

agcgacgaga acaaguucaa uucucgccuc uccacguauc acgaccugaa aaccaucgug     1860

ggggaugccg ucgacgaugu ggacaagcag gcugaucugg aaaaaugcau ugaguggagc     1920

acaaucuuug aagacggaaa gaucuacucu gccaagcuca acgagauuga uuggcugacc     1980

gaccagcaaa gaguucagcu ugcugccaaa cgguauagag gcugggggcg ccuguccgcg     2040

aagcuccuga cacagaucgu gaaugccaac ggacagcgga uuauggaucu gcuuugggac     2100

accacggaua auuucaugag aaucguccau agcgaggacu uugauaaacu cauuaccgaa     2160

gcuaaccaaa ugaugcuggc cgagaacgac gugcaggacg uuaucaauga ucuguacaca     2220

uccccgcaga acaagaaagc ucuccgccaa auccugcuug uggucaauga cauucagaag     2280

gccaugaagg gccaggcccc cgaaagaauc cugauugagu ucgcucggga agaugaggug     2340

aacccucgcc ucucugugca gagaaaacgg caaguugagc aggucuauca gaauaucagc     2400

aacgaacugc ugaauaacac cgagauucgc aaugaacuua aggaccucuc caacucugcg     2460

cugagcaaua caagacuguu ccucuacuuu augcaagggg gacgggauau guauaccggc     2520

gacucccuga acaucgaucg ccuuagcacg uacgacaucg aucacauucu gccccaaucu     2580

uucaucaaag acaauucccu cgauaacaga gugcugguga gccagcggau gaaucgcucu     2640

aaggccgacc aggucccuac agacuuuacc uccguggagc ugggccagaa aaugcaaauu     2700

cagugggaac agaugcuuag agcugggcug aucacaaaga agaaauacga uaaccucacc     2760

cugaaccccg accauauuag caaguaugcc augaaaggau ucaucaaucg gcagcucguu     2820

gagaccagac aagugaucaa gcuggccaca aaccuucuga uggagcagua cggcgaagau     2880

aauauugagc ucaucacggu caaaucuggg cugacccacc agaugcgcac agaauuugac     2940

uucccgaaga accggaaucu gaacaaucau caccacgcuu uugaugccua ucuuaccgcg     3000

uucgugggac uguaccuccu gaagagauau ccuaaacuca agcccuacuu uguuuauggc     3060

gaguaccaaa aagcuagcca gcaggacaag uggcgcaacu ucaauuuucu gaacgggcuu     3120

aaaaaggaug agcuggugga cgaaaauaca gaggccguca uuuggaacaa agaauccggc     3180

cucgccuacc ugaauaagau cuaucaguuc aagaaaauuc uggugacccg ggagguucau     3240

gaaaacagcg gagcucuuuu uaaucaaacg cuguacgccg cgaaggauga caaagccucu     3300

ggccaagggg gaaagcagcu caucccugcc aaacaggaua gacccacagc ccuguauggc     3360

ggguacuccg gaaagaccgu ggcuuauaug uguauugucc gcaucaagaa caaaaagggc     3420

gaccucuaca aagugugcgg gguggagacc agcuggcugg cccagcuuaa gcagcugaca     3480

gaugaggacu ccaaaaaggc uuuccugaag cagaaaaucu cuccucaguu caccaagguc     3540

aaaaagcaaa aaggcacaau uguuaaggug guggaagacu uugaggucau cgcuccccac     3600

auucucauca accagcgguu cuuugauaau ggacaggaac ugacgcuugg cagcgcgacc     3660

uauaagcaua acgagcaaga acugauucuc gacaaaacag ccgugaagcu gcucaauggg     3720

gcucugccgc uuacccaguc cgaggagcug gccgaacagg uuuacgauga gauccuggac     3780

caggugaugc acuacuuccc ucucuaugau acaaaccaau uuagagcuaa acugucugcc     3840

ggaaaggccg cuuucaugaa acuucccugg aaaagccagu gggacggcaa uaagaugguc     3900

cagguggggc agcaaguuau ccuggaucgc gugcucauug gacugcacgc gaacgccgcu     3960

gucuccgacc ucggcgugcu gaaaaucucu acgccccuug ggaagaugca gcagccuagc     4020

ggcauuuccc ugagcccgga uacccagauc auuuaccaau cucccaccgg acuguuugaa     4080

cggagaguug cccuccggga ccuguga                                         4107


<210>  4271
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4271
augagcagac ccuacaacau cggccuggac auugggaccu ccucuaucgg auggagcgug       60

gucgaugacc aguccaagcu cguuucugug cggggcaaau auggguacgg agugcgccug      120

uaugaugagg gccaaacagc cgcugaaaga cggagcuucc gcacgaccag acggcgccuu      180

aagagacgga aauggcgccu ggggcuccug agagagauuu uugaaccuua caucacaccg      240

gucgacgaua ccuucuuucu ucggcagaag caguccaauc ugucucccaa agaccagcgc      300

aagcucuauc cucaaacaag ccuguucaac gauagaaccg accgggccuu uuacgaugac      360

uaucccacaa uuuaccaccu ucgcuacaag cucaugaccg agaaaagaca guucgauauc      420

cgggaaaucu aucuggcgau gcaucacauu gugaaguaca gaggacauuu ucuuaaugag      480

gcuccuguuu ccagcuucaa aucuuccgag aucaaccugg uggcccacuu ugaccgccuc      540

aauacgauuu ucgccgaucu guuuagcgaa ucuggcuucc agcuugagac cgacaagcug      600

gcugaaguca aagcccuccu gcuggacaac cagcaauccg cuagcaaucg gcagagacag      660

gcccucucuc ugaucuauac acccuccacc aacaaggcgg uggagaagca gaauaaagcc      720

auugcuacag agcuucugaa ggccauccuc ggccugaaag cuaaguuuaa cguucuuacc      780

cgcauugaag ccgaggaugu gaaagcgugg acgcugacau ucaaugccga aaacuucgac      840

gaggaaaugg ucaagcucga gagcucccug gaugacaaug cucaucaaau caucgagucu      900

cugcaggaac ucuacagcgg gauucugcuu gccggaaucg ugccugagaa ccagucccug      960

agccaagcua ugauuaugaa auaugaugac caccagaagc accucaagau gcugaaagcc     1020

gugcgggaag cccuugcucc ggaggauaga cagcgccuga agcaggcgua cgaccaauau     1080

gucgauggcc aggaaaauac caaagccuac ucuaaggagg acuuuuacgg ggauaucaca     1140

aaagcucuca agaacaaccc cgaccauccu auuguuuccg agaucaagaa acugaucgaa     1200

cuggaccagu ucaugcccaa gcagcggacc aaagauaaug gagccauucc ucaccaacuc     1260

caucagcagg agcuggacag aaucauugaa aaccagcaac aguauuaccc cuggcuugcc     1320

gagcugaauc cgaacagcaa gcgccagacc guggcuaaau auaagcucga ugaacuggug     1380

gccuuucggg ucccuuacua ugugggcccc cugaucacag cggagcagca aagacagucu     1440

agcgacgcua aguucgccug gcuuauucgc aaagccgagg ggcggaucac gccuuggaau     1500

uuugaugaca agguugauag acaggcuucc gccaacgagu ucaucaaacg gaugaccaca     1560

accgacacau accuccuggc ugaggaugug cugcccaagc aaucucucau uuaucagcgc     1620

uuugaagucc ugaaugagcu uaacggacug aaaaucgacg aucagcccau uaccacggag     1680

cucaagcagg ccaucuucac agaccuguuu augcaaaaga ccagcguguc cguuaaaaau     1740

auucaggauu accuggugag cgaaaagaga uacgcgucuc ggccugccau caccggccuu     1800

uccgacgaga acaaauucaa uagcagacuc ucuacauauc acgaccugaa ggcuauuguc     1860

ggcgaugccg uggaagacgu cgauaaacag guggaccugg agaagugcgu ugaauggucc     1920

accaucuuug aggaugggaa aaucuacagc gcuaagcuca acgagauuga cuggcugaca     1980

gaucagcaac gcgugcagcu ugccgcgaag cgguauagag gauggggccg ccuguccgcc     2040

aaacuccuga cgcagaucgu caaugcuaac gggcagagaa uuauggaccu gcuuugggau     2100

accacagaca auuucaugcg gaucgugcac ucugaagauu uugacaagcu cauuaccgag     2160

gccaaccaaa ugaugcuggc ugaaaacgau guucaggacg ugaucaauga ccuguacaca     2220

agcccgcaga acaaaaaggc ccuccgccaa auccugcuug ucgugaauga uauucagaaa     2280

gccaugaagg gacaggcucc cgagagaauc cugauugagu ucgcgcggga agacgaggug     2340

aacccucgcc ucuccguuca gagaaagcgg caagucgaac agguguauca gaauaucucu     2400

aacgagcugc ugaauaacac cgaaauucgc aaugagcuua aagaucucag caacuccgcc     2460

cugagcaaua cgagacuguu ccucuacuuu augcagggcg ggcgggacau guauaccgga     2520

gauucucuga acaucgaccg ccuuuccaca uacgauaucg accauauucu gccccaaucu     2580

uucaucaagg auaauagccu cgacaacaga gugcuggucu cccagaaaau gaaucggagc     2640

aaggcugauc aggugccuac cgacuuuaca ucuguugaac ugggcaaaaa gaugcagcuu     2700

caaugggagc agaugcugag agccggccuc auuaccaaga aaaaguacga caaccugacg     2760

cucaaccccg aucacaucag caaauaugcc augaaggggu ucauuaaucg ccagcuggug     2820

gagacacggc aggucaucaa acuugcuacc aaccugcuca uggaacaaua cggagaggac     2880

aauaucgaac ugauuacagu gaaguccggc cugacccauc agaugagaac cgaguuugau     2940

uucccgaaga accgcaaucu uaacaaucac caucacgccu uugacgcgua ucugacagcu     3000

uucguugggc ucuaccugcu caaacgguau ccuaagcuga aacccuacuu uguguaugga     3060

gaguaccaga aggccagcca acaggauaaa uggagaaacu ucaauuuucu uaacggccug     3120

aagaaagacg aacucgucga ugagaauacg gaagccguga ucugggacaa ggagucuggg     3180

cuggcuuacc ugaacaagau uuaucaguuc aaaaagaucc uuguuacccg cgaagugcac     3240

gaaaauuccg gcgcccuguu uaaccagaca cucuacgcgg cuaaagauga caaggccagc     3300

ggacaaggcg ggaaacagcu gauuccugcc aagcaggauc ggcccaccgc ucucuaugga     3360

ggcuacuccg ggaagacagu cgccuauaug uguaucguga gaauuaaaaa uaagaaagga     3420

gaccuguaca aggugugcgg cgucgagacc ucuuggcuug cucagcugaa acaacugacg     3480

gaugaagaca gccagaaggc cuuccucaag cagaaaaucu ccccucaguu cacaaagguu     3540

aaaaagcaaa aagggaccau cgugaaggug gucgaggacu uuaaggugau ugcgccccau     3600

auccugauua accagcgcuu cuuugauaac ggccaggaac uuacccuggg aucugccaca     3660

uauaaacaca augagcaaga acucauccug gacaagaccg cuguuaaacu ccugaacggc     3720

gcccuuccgc ugacacagag cgaggagcug gcugaacagg uguacgauga gauucucgac     3780

caggucaugc auuacuuccc ucuguaugau acgaaucaau uucgggccaa gcuguccgcc     3840

gggaaagcug cguucaugaa gcugcccugg aagucuaucu gggacggaaa caaaaugacc     3900

cagaucggcc agcagauuau ccucgaucgg auucugaucg ggcuccacgc caaugcugcc     3960

gugagcgacc ugggaguucu uaagauuucc acaccccugg gcaaaaugca acagccuagc     4020

gggaucucuc uguccccgga uacccagauc auuuaccaga gccccacagg ccucuuugaa     4080

agacgcgugg cccugagaga ccuuuga                                         4107


<210>  4272
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4272
augguggaga agaaaagcua cuccaucggc cuggacauug ggaccaacuc ugucggaugg       60

gccguuauca cagaugacua uaaggugccc gcuaaaaaga ugaaagugcu cggcaauacg      120

agcagacagu ccauuaagaa gaacaugauc ggggcccugc uuuucgauga aggaggcccu      180

gcggcuucua cccgggucaa acgcacaacc agacggcgcu acacaagacg gaagaaucgc      240

cugugcuauc ucagagacau uuuugagagc gaaaugcaca ccaucgauaa acauuucuuu      300

cugcggcuug aggacuccuu ccugcacaag ucugauaaac gcuacgaagc ccauccgauc      360

uuugggacac uccaagagga gaaggccuau cacgacaacu accccaccau uuaucaucug      420

agaaaggcuc uggccgauaa uacggaaaaa gcugaccucc ggcugaucua ccuugcccug      480

gcgcacauua ucaaguuccg cggacacuuu cucauugagg gcgcccugag cgcuaacaau      540

accgaugugc agcagcuugu ucaugcccug guggacgcuu acaacaucau guucgaagag      600

gaucagcucg acauugaggc caucgacguc aaagcgaucc ugacagaaaa gauuuccaaa      660

accagacggc uggagaaucu caucagcaac auuccuggcc aaaagaaaaa ugggcuguuu      720

ggaaaccuua ucgcccuguc ucucggccug acacccaauu ucaaguccca cuuuaaccuu      780

ccugaagaug cuaagcugca gcucgccaaa gacaccuaug augaggaacu gaauaaccug      840

cucacgcaga uuggggacga guacgcugau cuguuccuua gcgccaagaa ccugucugac      900

gccauccucc uguccgauau ccuuacagug aauggagacg gcacccaggc uccccugagc      960

gcgucucuca uuaaacgcua ugaggaacau agacaagauc uggcccugcu caagcagaug     1020

uuuaaagagc agcugccuga ccuuuaccgg gauguuuuca cagacgaaaa caaggacggg     1080

uaugcuggau acaucuccgg caagaccagc caggaggccu ucuauaaaua cauuaagccg     1140

auccuggaaa cccucgaugg cgccgaggac uuucugacaa aaauuaauag agaggauuuc     1200

cugcgcaagc aacggacguu ugacaacggg uccauccccc accagaucca ccuuggagaa     1260

cuccaggcua uucuggagag acaacaggcc uacuauccuu uccugaaaga uaaucaggaa     1320

aagaucgaga agauucucac cuuucgcauc cccuacuaua uuggcccucu ggcgcggggg     1380

aacucuagau ucgcuuggcu uacacgcacc agcgaccaga aaaucacacc cuggaauuuu     1440

gaugaaaugg uggaccaaga ggccuccgcc caggcuuuca uugagcggau gaccaacuuu     1500

gaugaauacc ugccgcagga gaagguccuc ccuaaacaua gccugacgua ugaauacuuc     1560

acaguguaua augagcugac caaggugaaa uacgucaccg agggaaugac aaagcccgaa     1620

uuucuuucug ccggccagaa ggagcaaauc guugaacucc uguucaaaaa guacagaaaa     1680

gugaccguga agcagcugaa agaggacuuu uucuccaaga ucgaauguuu cgauacaguc     1740

gacauuagcg ggguggagga uaaauuuaac gcuucucucg gaacguauca cgaccugcuu     1800

aagaucauua aggacaaagc cuuccuggau aauagcgaga acgaaaauau cauugaggac     1860

aucauccuca cccugacacu guuugaagau aaggagauga uugcgaaccg ccuugccguu     1920

uacgaagacc ucuucgauca gaaugugcug aaacagcuga agcggagaca uuauaccggc     1980

uggggccgcc ucuccaaaca acugaucaac gggaugcggg acaagcacac aggaaagacc     2040

auucuugauu uucugaaagc ugacggcuuc aucaacagaa auuuuaugca gcucauuaac     2100

gaugacaauc ugucuuucaa ggaggagauc aaaaaggccc aggaaggggg acugaaagau     2160

agcaucaacg accagauucg ggaucuugcu ggcuccccug ccaucaagaa ggggauucuc     2220

caaacgauca auauugucga cgagaucgug aaaaucaugg gcaaggcgcc ccagcauauu     2280

guuguggaaa uggcccgcga cguccagaaa accgauaucg gagugaagca aagcagagag     2340

cggaugaaaa gaguccagga agugcugaag aagcugggcu cucagcuccu gaaagagcac     2400

cccguugaga acuuucagcu ucaaaaugaa cgccuguacc ucuauuaccu gcagaacggg     2460

aaggacaugu auacaggaga ggaacugucc auuagcaauc uuucucacua cgauaucgac     2520

cauauuaucc cucgguccuu cauuaaagau gacagcaucg auaacaaggu gcugaccaga     2580

uccgagcaca aucgcggcaa aacagacaac gucccgucua ucgagguggu uaagagaaug     2640

aaacccuacu ggcagaagcu ccuggauacc aaggugauua gccagcggaa auuugacaau     2700

cucacgaagg cugaacgcgg gggacugcaa gaguccgaua aagccaacuu cauccagaga     2760

cagcuugucg aaacacggca gauuaccaag cauguggcuc aaauccugga cucucgcuuu     2820

aauacagaga gagacgaaaa agaucggccu auucgcagag ugaagguuau cacccucaag     2880

agcaaauucg ucuccgacuu ccggcaggau uuuggcuucu auaagcugcg cgagaucaac     2940

gacuaccacc acgcccauga ugccuaucug aacgcugugg uggggaccgc gcuucugaaa     3000

auguacccca agcucgccag cgaguuuguc uauggcgacu accagaaaua ugaucugaag     3060

agaauggugg gaaagucugg caaagccucc gggcacgcca cagccaagua cuucuuuuac     3120

agcaaucuca ugaacuucuu uaaaucugaa guuaagcugg cuaauggaaa cauuaucaaa     3180

cgguccccua uugaggugaa ugaagagacg ggcgaaaucg ucugggacaa gaccaaggau     3240

uucgggacag ugagaaaagu ucuuagcgcu ccccaaguga acauugucaa gaaaaccgag     3300

auccagacag gaggcuuuuc uaaugagacc auccugagca aggggaaauc cagcaagcuc     3360

auuccgcgca agaacaaaug gcgggacacg acaaaguaug gcggauucaa uaccccuacc     3420

guggcguacu cuguucuggu ggucgcuaaa guggaaaagg gcaaagccaa gaaacugaag     3480

cccgugaagg agcuugucgg gaucacaauu auggaaagaa ccaaauuuga ggccaacccu     3540

aucgcuuucc uggaauccaa gggauaucau gauauucagg agcaccucau gaucacacug     3600

cccaaauaca gccucuucga gcuggaaaau ggccgccgga gacuucuggc cuccgcgacg     3660

gagcugcaga aggggaacga aaugguucuc ccucaacauc uggugaccuu ucuuuaucgc     3720

gugucuaaac gggacaaggg aacacagagc gagaauaugg aguacauuuc caaccacaag     3780

gaaaaauuca ucgagaucuu ucacuauauu aucagauacg cugaaaagaa ugucauuaaa     3840

cccaagguga ucgagcgccu gaacgauacc uucaaccaga aauuuaauga cucugaccuc     3900

acagaacuga gcauuuccuu ccucaaccug uuuaaguuca ccucuuuugg cgccccggag     3960

aaguucacgu uucuuaauag cgagaucaaa caggaugacg uucgguacag auccaccaag     4020

gaaugccuga acagcacacu gauccaucaa ucugugaccg ggcucuauga gacacggauu     4080

gaucuguccc aguucggcgg agacuga                                         4107


<210>  4273
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4273
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcugggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcg cuucuguuug auuccggaga gaccgccgaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuugc      240

uaucugcagg agaucuucuc uaacgaaauc gccaaagugg acgauagcuu uuuccauaga      300

cuugaggaau ccuuucuggu cgaggaggac aagaaacacg aacggcaucc uauuuucggc      360

aauaucgugg augagguugc cuaccacgaa aaguauccga ccauuuacca ucuccgcaag      420

aaacuggcug acucuacaga uaaggccgac cugagacuca ucuaucuggc ucuugcccac      480

augauuaaau uucgggggca cuuccugauc gagggagauc ucaaccccga caauagcgac      540

guggauaagc uguuuauuca acuuguccag accuacaacc agcuguucga ggaaaauccu      600

aucaacgcgu cccgcgugga cgccaaagcu auccucagcg ccagacuguc uaagucccgg      660

cgccuggaga aucucauugc ucagcugccc ggcgagaaaa gaaacggccu uuuugggaau      720

cugaucgccc ucagccuggg acuuacgccu aacuucaagu cuaauuuuga ucuggcggaa      780

gacgccaagc uccaacuguc caaagauacc uacgacgaug accuggauaa ccuccuggcu      840

cagauuggcg accaguaugc cgaucuuuuc cuggcugcca agaaccucag cgacgccauc      900

cugcuuucug acauucugcg gguuaauaca gaaaucacca aagcuccccu cuccgcgagc      960

augaucaaga gauacgauga gcaucaccag gaccugacac ugcucaaagc ccuggugcgc     1020

caacagcuuc cugagaagua uaaggaaauu uucuuugauc aguccaaaaa cggguacgcu     1080

ggauauaucg acggcggggc cucucaggag gaguucuaca aguuuauuaa accgauccug     1140

gaaaagaugg auggaaccga ggaacuccug gccaaacuga aucgggagga ccuucucaga     1200

aagcaacgca cguucgauaa cggcagcauu ccccaucaga uccaccuggg cgaacugcac     1260

gcuauccucc ggagacagga ggacuuuuau ccuuuccuga aggauaaucg cgagaaaauu     1320

gaaaagaucc uuacauuucg gauucccuac uacgucgggc cucuggccag aggaaacucc     1380

cgcuucgcgu ggaugacccg gaaaagcgaa gagacaauca cccccuggaa uuuugaggaa     1440

gugguggaca agggcgcuuc ugcccaaucc uucauugaga gaaugaccaa cuuugauaaa     1500

aaucucccga acgaaaaggu ccugccuaag cauagccugc uuuaugagua cuucacaguu     1560

uauaaugagc ucacgaaagu gaaguacgug accgaaggga ugcgcaaacc cgccuuucug     1620

ucuggagagc agaagaaagc uaucgucgac cugcuguuca agacaaaccg gaaagugacc     1680

guuaagcagc ugaaggaaga uuauuucaaa aagauugagu guuuugacag cguggaaauc     1740

uccggcgucg aggacagauu caaugccucu cuugggacau accacgaucu gcucaaaauc     1800

auuaaggaca aagauuuucu ggacaacgag gaaaaugagg auauccugga agacauugug     1860

cuuacccuca cgcuguucga ggaucgggaa augaucgagg agcgccugaa gacauaugcu     1920

caucucuuug acgauaaggu uaugaaacag cugaagagac ggagauacac cggauggggc     1980

cgccuuagcc ggaaacugau uaacggcauc agagacaagc aauccgggaa aaccauccuc     2040

gauuuccuca agagcgacgg auuugccaac cgcaauuuca ugcagcugau ucacgaugac     2100

ucucuuacau uuaaggaaga ccuccagaaa gcucaggugu ccggccaagg ggauagccug     2160

caugagcaca ucgccaaccu ggcuggaucu ccugccauua agaaaggcau ccuccagacc     2220

gucaaggugg ucgacgaacu ggugaaaguu auggggagac acaagcccga gaauauugug     2280

aucgaaaugg cucgggagaa ccagacaacg cagaagggcc agaaaaauuc ccgcgagaga     2340

augaagcgga ucgaagaggg aauuaaagaa cuuggcagcg auauccugaa ggaguacccc     2400

gucgagacca cacaacucca gaacgaaaaa cuguaucugu acuaucuuca gaaugggcgc     2460

gacauguacg uggaucaaga gcuggacauu aacagacucu ccgauuauga cguugaucau     2520

aucgugccuc agucuuuccu gaaggacgau agcauugaca auaaaguccu cacccggucc     2580

gacaagaacc gcggaaaguc ugauaaugug ccgagcgaag aggugguuaa aaagaugaaa     2640

aacuacugga agcagcugcu uaaugccaaa cugaucacac aaagaaaguu ugacaaccuc     2700

accaaggcgg aacggggcgg gcuguccgag cuggauaaag ccggauucau caagcgccag     2760

cuugucgaga cgagacagau uaccaaacac guggcucaga uccuggacag ccggaugaau     2820

acaaaguaug augaaaacga caaacucauu agagaggugc gcgucaucac ccugaagucu     2880

aagcucgugu ccgauuuucg gaaagacuuc caauucuaca agguuagaga aauuaacaau     2940

uaccaucacg cccacgaugc uuaucugaac gccguggucg gcacagcccu uaucaaaaag     3000

uaccccaaac uggagagcga auuuguguau ggggacuaca agguuuauga uauucgcaag     3060

augaucgcua aaucugagca ggagaucggc aaggcgaccg ccaaauacuu cuuuuauucc     3120

aauauuauga acuucuuuaa gacggaaauc acacucgcua auggagagau ucggaaaaga     3180

ccucugaucg aaaccaacgg cgagacaggg gaaauugugu gggacaaggg acgcgauuuc     3240

gccaccgucc ggaaggugcu gagcaugccc cagguuaaua ucgugaaaaa gaccgagguc     3300

cagacaggcg gguuuucuaa agagagcauc cuuccuaaga gaaacuccga caaacugauu     3360

gcccgcaaga aagacuggga ucccaagaag uacggaggcu ucgacagccc gacgguggcu     3420

uacucugugc ucgucguugc caaaguggaa aaggggaaau ccaagaaacu caagagcgug     3480

aaggagcugg ucggcaucac cauuauggaa cgguccucuu uugagaaaga uccuguggac     3540

uuccuggagg cgaagggaua uaaagaaguu agaaaggauc uuaucauuaa acugcccaag     3600

uacagccugu uugagcucga aaauggccgg aagcgcaugc uggcuuccgc cggggagcuu     3660

caaaaaggaa acgaacuggc ccucccuucu aaguauguga auuuccugua ccucgcuagc     3720

cauuaugaga aacugaaggg cucccccgag gacaacgaac agaaacagcu uuucgucgag     3780

cagcacaagc auuaccugga ugaaaucauc gagcaaauuu cugaauuuag caagagagug     3840

auccuggccg acgcgaaucu cgauaaaguu cuguccgcuu auaacaagca ccgggacaaa     3900

ccuauucgcg agcaggccga gaauaucauu caucuuuuca cacugaccaa ccucggggcc     3960

cccgcugccu uuaagugcuu cgauacaacc aucggaagaa accgguacaa aagcauuaag     4020

gaagugcugg acgcuacgcu cauccaccag ucuaucacag gccuguacga gaccagaauu     4080

gaucuuuccc aacucggggg cgacuga                                         4107


<210>  4274
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4274
auggacaaga aauacagcau cggccuggau auugggaccu ccucuaucgg auucgugggc       60

gucgacgaga acggguggcc ccucaauauu aagggaaaaa gagccaucgg cgcucaccug      120

uuucgggaag gggaggccgc ggcugaaaca cgcggauuca gaacgacccg gcgcagauau      180

aaccggcgca aguggagacu ucggcugcuc cgcgagauuu uugaugaacc uaucagcaga      240

guugacccga cauucuuugc ccggaugaaa gaguccucug ugagccccaa ggauccuaau      300

uacgacggcc uggccaagga gauccuuauu ccccgcgaua aagacggcaa gauccuguau      360

ccuaccauuu accaucucag acacgcucug auguaugaag aucaucaguu cgacauccgg      420

gagauuuacc uggccaucca ccauauugug aaauaucgcg ggcacuuucu ccuggaugga      480

gcugccaaga acuucgaauc cucuaaucuu gaccugaaaa gcgauaucca cgagaucaac      540

gcgauucucg ucggcaucuu ugggaaugag caagugcagu ucaacgacca guacguugaa      600

aagcugaagg aggugcuucu ggacuccaca cagagcagau cugaucggca aaaagccgcu      660

gccaagcucc ugcugcccac caaugaccuc gauaaagaca caaccaagau ucugaaaacg      720

gucuccaccg aaaucacaaa ggcuauuacc ggacuuaaag ccaaguuuuu cgugaucaca      780

ggcaacgaua ucgcgaagga ccagcagaaa gauuggaccu uuagcauuga cacgcuggau      840

gagaagaugu cuguuaucga accuuccuuc agcgagaaaa auuucucucu cauuacaauc      900

cugcagaaac uuuacuccag cgugacccug gccgggauug ucccgaacgg acucucccug      960

ucugaggcua ugauccaaaa auaugacaga caugccagcg aucuggugcu ccugaagaag     1020

uacugggaca caaaacccaa ggugcuuaaa caccagcugc agaauaccua ugcugaauac     1080

aucgagauuc aucaggucgu ugacgaauuu uauuccgauc ucagcaagcu gaucaaaaac     1140

gacgaugaca cccuugccca agagauuaag ucuaagauca caauuggcaa uuuccugccu     1200

aaacagcgca cgaccgaaaa cggcgugauc cccuaccagc uccaacagcg ggagcuggau     1260

acaauuauca agaaccagca ggaguauuac ccuuggcugg ccgaagagaa ucccguggcu     1320

gaccaccaaa ucgcguuucc guacaaacuc gaugaacugg ucggguuccg cauuccuuau     1380

uacgugggac ccaugaucgc caaggaggac accuccaaca gcaaaaauuc uaaguuugcu     1440

uggaugauua gaaagcagag cggcgaaguu acaccuugga acuucgauga gaaaaucgac     1500

cggaccgccu ccgccacggc uuuuauugau agaaugacaa ccaccgacac auaucuuauc     1560

ggggaggaug ugcugcccaa gcagucucuc aucuaccagc gcuucgaagu ccugaaugag     1620

cugaacaaaa uuagcaucga cggacaaccc auuacccgga agcagaaaca guccguguau     1680

acacacguuu uugaaaagaa aaagacggug caggucaagg acgugcaaaa auuccuuguc     1740

agcucuggcg aggugauguc ccucccuaag aucgggggac uggccaauga gaaacaguuu     1800

aacagcucuc uguccacccu cuacgauuau aauaagauua ucccggacgc gcuggaagau     1860

agaaacaaag uugacgauau cgagaagauu auccuuugga gcacaauuuu cgaagacaau     1920

uccaucuuug uggagaagcu ggauucuauu gacuggcuca ccagcuccca gcgcaaacgg     1980

cuggcucaga agagauaccg cggcuggggc cggcugucuc uuagacuccu gacaaccuuc     2040

aaaaacaagg auggguacag cauccuggac gaacucugga acuccaauca uaauuucaug     2100

gagauccaag cccagagcga uuuuucugag cagauugcca cggcuaacaa caauaaccug     2160

ucccaagucc uucccgccaa augggaaccu ggacggaaga aucuggcuag ccucgacgag     2220

uucaucucua accuguauac cagcccccag aauaagaaag cccugcgcaa ggcgauuaga     2280

gugguucagg auaugaugac agccgcuggc cacaugccug aaaagaucuu uauugaggug     2340

gcccgggaca ccuccaacuc ucaucagcaa acaaucgcua gacagcgcca gcuucagcaa     2400

cucuaccaga gcgaagacaa ggaggucauu gauuccagcg ugcggucuga gcuggacgau     2460

auccugaaaa auagagccaa caucaaggac cagcuguucc ucuauuuuca gcaaggggga     2520

cgcgaucuuu acaccggcaa ggcgauuaau aucgacaaac ugucccucua ucacauugau     2580

cauauccugc cccagagccu ggugaaggac gauucccuug acaacagagu ucucgucucu     2640

cagaaaacga augaagauaa gggggugaca uucgugagcg acaaauacau gaacaauaug     2700

cugccgaccu ggaagcggug gcacgagaug ggccugauuu ccgcccgcaa acucaagaac     2760

cugacaauga gaccugacga aaucgauaag uaugcucccg gauuuaucca acggcagcuu     2820

gucgagaccc gccaggugac caaacugguu auugaacucc uggacgcccu gcuuggcaau     2880

ucucagacag agaucauuac gaucaaggcu agccuguccc accaauucag acagcaguuu     2940

gaucucguga aaauucggga ggucaacgac aaccaucacg ccuucgaugc cuuucuggcu     3000

gcgcgcaucg ggaccuaccu cuacaagaga uauccuaaac ugcggcccua cuucguguau     3060

ggacaguaca ugcaaaauca uaagaaccuu aagucuauca aucugauuaa aacacucgac     3120

agcgauaagg ccaucguuga cgaaggcacc ggggagauuc ugugggauaa aaaguccgac     3180

cuggcuaaaa ugaucaagau uuauaacuac cagcgccuuc ugaucacaca cgaaagcaga     3240

accucuuuug gagccaugua uaagcagaaa auucucaagg ccacggaugc uaaacagaau     3300

ggcgggggcu ucaagcuggu gccuacaaaa cggaacagac ccgucgacau cuacggaggc     3360

uacaccgggc aaaccgccgc guaucucaca aucgugcgcg uuuacuccaa gaagaccagc     3420

gagcugcggg ugcuuccgau uuauacaaau aaaguccagc agaucgagaa ggcuaugacg     3480

cagggagaua aagccaccau gcuggccuuc aagaaaguga uugcuccuca acucggcaag     3540

gaaaacaaga aaacacacga gaucaagauu cccgaauuug acguggucau ccccaaaguu     3600

cugcuggacc agcuugugau cgaugacauu gccgggucuc cucauagauu ccgccuggga     3660

accgauacau acuaucacaa uucccagcaa cucuuucugc cgcucagcau gcagcggcag     3720

cugauggaca gagagucuga ugacaaggug uaccuugaua ucuucaacga cguccugaaa     3780

cagguggauu ccuauuuuaa ucuguacgac guuaacggcu uccgcgcggc ucuccaaaag     3840

agcaaaaagg ccuuugaaca gcugcccauu aaugauuucc cuaaggacgu gcccgauaac     3900

aaaaagaaaa aggggaaacg guccauccuu aaggagauuu uuaucggccu gcaugccaau     3960

gcuacccucg gaaaccugaa gcagaugggc aucaaaacgg acuucgggaa gcuccagaaa     4020

agcaacggac ugagaauuac cgagaauugc aagaucauuc accaaucucc uacaggccuu     4080

uuugaacgca ucguccccau uaaauga                                         4107


<210>  4275
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4275
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug auuccggaga gauugccgaa      180

gcgacccggc ucaaacgcac agcuagacgg cgcuacacca gacggaagaa ucgcaucugc      240

uaucugcagg agaucuucuc uaacgaaaug gccaaagugg acgauagcuu uuuccauaga      300

cuugaggaau ccuuucuggu cgaggaggac aagaaacacg aacggcaucc uauuuucggc      360

aauaucgugg augagguugc cuaccacgaa aaguauccga caauuuacca ucuccgcaag      420

aaacuggugg acucuaccga uaaggcugac cugagacuca ucuaucuggc ccuugcucac      480

augauuaaau uucgggggca cuuccugauc gagggagauc ucaaccccga caauagcgac      540

gucgauaagc uguuuauuca acuugugcag acauacaacc agcuguucga ggaaaauccu      600

aucaacgccu ccggcguuga cgcgaaagcc auccucagcg cucgccuguc uaaguccaga      660

cggcuggaga aucucauugc ccagcugccc ggcgaaaaga agaacgggcu uuuuggaaau      720

cugaucgcuc ucagccuggg ccuuaccccu aacuucaagu cuaauuuuga ucuggccgag      780

gacgcgaaac uccaacuguc caaggauacg uacgacgaug accuggauaa ccuccuggcc      840

cagauugggg accaguaugc ugaucuuuuc cuggccgcua aaaaccucag cgacgccauc      900

cugcuuucug acauucugcg ccucaauucc gaaaucacca aggcuccccu gagcgcuucc      960

augaucaaaa gauacgauga gcaucaccag gaccugacac uccugaaggc gcuugugcgg     1020

caacagcugc cugagaagua uaaagaaauu uucuuugauc agucuaagaa cggauacgcc     1080

ggcuauaucg acgggggagc uagccaggag gaauuuuaca aauucauuaa gccgauccuc     1140

gagaaaaugg auggcaccga ggaacugcug gccaaacuua auagagagga ccuccugcgc     1200

aagcaacgga cauucgauaa cggcuccauu ccccaucaga uccaccuggg ggaacuccac     1260

gccauccuga gacgccagga ggacuuuuau ccuuuccuua aagauaaucg ggaaaagauu     1320

gagaaaaucc ugaccuuucg cauucccuac uacgucggac cucucgcuag aggcaacagc     1380

cgguucgccu ggaugacgag aaagucugaa gagacaauca cccccuggaa uuuugaggaa     1440

gugguggaca aaggggcguc cgcucaaucu uucauugagc gcaugacaaa cuuugauaag     1500

aaucugccga acgaaaaggu ccugccuaaa cauagccuuc ucuaugagua cuucaccguu     1560

uauaaugagc ugaccaaggu gaaauacgug acagaaggaa ugcggaagcc cgccuuucug     1620

agcggcgagc agaaaaaggc caucgucgac cuccuguuca aaacgaacag aaaggugacc     1680

guuaagcagc uuaaagaaga uuauuucaag aaaauugagu guuuugacuc cguggaaauc     1740

ucuggggucg aggaccgcuu caaugcuagc cugggaacau accacgaucu ccugaagauc     1800

auuaaagaca aggauuuucu ggacaacgag gaaaaugagg auauccuuga agacauugug     1860

cucacccuga cacuguucga ggaucgggaa augaucgagg agagacucaa gaccuaugcc     1920

caucuguuug acgauaaagu uaugaagcag cuuaaacggc gcagauacac gguguggggc     1980

cggcugucca gaaagcucau uaacggcauc cgcgacaaac aaagcgggaa gacaauccug     2040

gauuuccuga agucugacgg auuugcuaac cggaauuuca ugcagcuuau ucacgaugac     2100

ucccucaccu uuaaagaaga cauccagaag gcccagguca gcggccaagg ggauucucug     2160

caugagcaca uugcgaaccu ggccggaucc ccugcuauca aaaagggcau ucuccagacc     2220

gugaaagucg uggacgaacu gguuaaggug auggggagac acaagcccga gaauaucguc     2280

aucgaaaugg cccgcgagaa ccagacaacc cagaaaggcc aaaagaauag cagagagcgg     2340

augaaacgca uugaagaggg aaucaaggaa cuuggcuccc agauucugaa agagcauccc     2400

guggagaaca cacagcucca gaaugaaaag cuguaccugu auuaccuuca aaacgggaga     2460

gauauguaug uugaccagga gcuggauauc aaucggcucu cugacuacga uguggaccac     2520

auugucccuc agagcuucau caaagaugac uccaucgaua acaaggugcu gacgcgcucu     2580

gacaagaaua gaggaaaaag cgacaacgug ccguccgaag agguugucaa gaaaaugaag     2640

aauuauuggc ggcaacuccu gaacgcuaaa cuuauuaccc agcgcaaguu ugauaaucug     2700

acaaaggccg aaagaggcgg gcucagcgag cuggacaaag cgggauucau caagcggcag     2760

cugguggaga cccgccagau uacaaaacau guggcccaaa uccuugauuc uagaaugaac     2820

accaaguacg acgaaaacga uaaacugauu cgggagguca aggugaucac gcucaagucc     2880

aaacugguua gcgacuuuag aaaggauuuc caguucuaua aagugcgcga aauuaauaac     2940

uaccaccacg cucaugacgc cuaccucaau gcugucgugg gcaccgcccu gaucaagaaa     3000

uaucccaaac uugagucuga auuuguuuac ggggauuaua agguguacga cguccggaag     3060

augcuggcca aguccgagca ggagaucggc aaagcuacag cgaaguauuu cuuuuacagc     3120

aacauuauga auuucuuuaa aaccgaaauc acacucgcca acggagagau uagaaagcgc     3180

ccucugaucg aaaccaaugg cgagacgggg gaaauugugu gggauaaggg acgggacuuc     3240

gcuacaguua gaaaagugcu gucuaugccc caggucaaca ucgugaagaa aaccgaggug     3300

caaacaggcg gguuuagcaa ggaguccauc cuuccuaaac gcaauagcga uaagcugauu     3360

gcccggaaaa aggacuggga ccccaagaaa uauggaggcu ucgauucucc gaccgucgcc     3420

uacuccguuc ucgugguggc uaaggucgaa aaaggcaaga gcaaaaagcu gaaguccgug     3480

aaagagcucc uggggaucac cauuauggaa agaucuagcu uugagaagaa cccuaucgac     3540

uuccuugagg ccaaaggaua caaggaaguu cgcaaagauc ugauuaucaa gcugcccaag     3600

uauucccucu uugagcugga aaauggccgg aaaagaaugc uugcgucugc uggggagcug     3660

cagaagggaa acgaacucgc ccugccuagc aaauacguga auuuccucua ucuggccucc     3720

cacuacgaga aacuuaaggg cucucccgag gacaacgaac agaagcagcu guucgucgag     3780

caacauaagc acuaucugga ugaaaucauu gagcagauca gcgaauuuuc caaacgggug     3840

auucucgcug acgccaaucu ggauaagguu cuuagcgcgu acaacaaaca ucgcgacaag     3900

ccuaucagag agcaggcuga gaacauuauc caccuguuca cacucacgaa ucugggggcc     3960

cccaccgccu uuaaauauuu cgauacaacc auugaccgga agcgcuacac aucuaccaaa     4020

gaagugcucg augcuacguu uauccaccaa uccaucacag gacuguacga gaccagaauu     4080

gaccuuagcc agcugggcgg ggauuga                                         4107


<210>  4276
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4276
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug auuccggaga gaccgccgaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuugc      240

uaucugcagg agaucuucuc uaacgaaaug gccaaagugg acgauagcuu uuuccauaga      300

cuugaggaau ccuuucuggu cgaggaggac aagaaacacg aacggcaucc uaucuucggc      360

aauauugugg augagguugc cuaccacgaa aaguauccga ccaucuacca ucuccgcaag      420

aaacuggugg acucuacaga uaaggcugac cugagacuca uuuaucuggc ccuugcucac      480

augaucaaau uucgggggca cuuccugauu gagggagauc ucaaccccga caauagcgac      540

gucgauaagc uguuuaucca acuugugcag accuacaacc agcuguucga ggaaaauccu      600

auuaacgccu ccggcguuga cgcgaaagcc auccucagcg cucgccuguc uaaguccaga      660

cggcuggaga aucucaucgc ccagcugccc ggcgaaaaga agaacgggcu uuuuggaaau      720

cugauugcuc ucagccuggg ccuuacgccu aacuucaagu cuaauuuuga ucuggccgag      780

gacgcgaaac uccaacuguc caaggauacc uacgacgaug accuggauaa ccuccuggcc      840

cagaucgggg accaguaugc ugaucuuuuc cuggccgcua aaaaccucag cgacgccauu      900

cugcuuucug acauccugcg ccucaauucc gaaauuacaa aggcuccccu gagcgcuucc      960

augaucaaaa gauacgauga gcaucaccag gaccugaccc uccugaaggc gcuugugcgg     1020

caacagcugc cugagaagua uaaagaaauc uucuuugauc agucuaagaa cggauacgcc     1080

ggcuauauug acgggggagc uagccaggag gaguucuaca aauuuaucaa gccgauucuc     1140

gagaaaaugg auggcacaga agaacugcug gccaaacuua auagagagga ccuccugcgc     1200

aagcaacgga ccuucgauaa cggcuccauc ccccaucaga uucaccuggg ggaacuccac     1260

gccauccuga gacgccagga ggacuuuuau ccuuuccuua aagauaaucg ggaaaagauc     1320

gagaaaauuc ugacguuuag aauccccuac uacgucggac cucucgcucg cggcaacagc     1380

cgguucgccu ggaugacaag aaagucugaa gagaccauua cacccuggaa uuuugaggaa     1440

gugguggaca aaggggcguc cgcucaaucu uucaucgagc gcaugaccaa cuuugauaag     1500

aaucugccga acgaaaaggu ccugccuaaa cauagccuuc ucuaugagua cuucaccguu     1560

uauaaugagc ugacaaaggu gaaauacgug acggaaggaa ugcggaagcc cgccuuucug     1620

agcggcgagc agaaaaaggc cauugucgac cuccuguuca aaaccaacag aaaggugaca     1680

guuaagcagc uuaaagaaga uuauuucaag aaaaucgagu guuuugacuc cguggaaauu     1740

ucuggggucg aggaccgcuu caaugcuagc cugggaaccu accacgaucu ccugaagauc     1800

aucaaagaca aggauuuucu ggacaacgag gaaaaugagg auauucuuga agacaucgug     1860

cucacacuga cccuguucga ggaucgggaa augauugagg agagacucaa gacguaugcc     1920

caucuguuug acgauaaagu uaugaagcag cuuaaacggc gcagauacac aggcuggggc     1980

cggcugucca gaaagcucau caacgggauu cgcgacaaac aaagcggaaa gaccauccug     2040

gauuuccuga agucugacgg cuuugcuaac cggaauuuca ugcagcuuau ccacgaugac     2100

ucccucaccu uuaaagaaga cauucagaag gcccagguga gcgggcaagg agauucucug     2160

caugagcaca ucgcgaaccu ggccggcucc ccugcuauua aaaaggggau ccuccagaca     2220

gucaaagugg ucgacgaacu ggugaagguu augggcagac acaagcccga gaauauugug     2280

aucgaaaugg cccgcgagaa ccagaccaca cagaaaggac aaaagaauag cagagagcgg     2340

augaaacgca ucgaagaggg cauuaaggaa cuuggguccc agauccugaa agagcauccc     2400

gucgagaaca cgcagcucca gaaugaaaag cuguaccugu auuaccuuca aaacggaaga     2460

gauauguaug uggaccagga gcuggauauu aaucggcucu cugacuacga uguugaccac     2520

aucgugccuc agagcuucau uaaagaugac uccaucgaua acaagguccu gacccgcucu     2580

gacaagaaua gaggcaaaag caacaaugug ccguccgaag aggugguuaa gaaaaugaag     2640

aacuauuggc ggcaacuccu gaaugcuaaa cuuaucacac agcgcaaguu ugacaaccug     2700

accaaggccg aaagaggggg acucagcgag cuggauaaag cgggcuucau uaagcggcag     2760

cuggucgaga cacgccagau caccaaacau guggcccaaa uucuugacuc uagaaugaac     2820

acgaaguacg augaaaauga caaacugauc cgggagguga aggucauuac ccucaagucc     2880

aaacugguga gcgauuuuag aaaggacuuc caguucuaua aaguucgcga aaucaacaau     2940

uaccaccacg cucaugaugc cuaccucaac gcuguggucg ggacagcccu gauuaagaaa     3000

uaucccaaac uugagucuga auuuguguac ggcgacuaua agguuuacga ugugcggaag     3060

augaucgcca aguccgagca ggagaucgga aaagcuaccg cgaaguauuu cuuuuacagc     3120

aauauuauga acuucuuuaa aacagaaauc acccuggcca auggcgagau uagaaagcgc     3180

ccucucaucg aaacgaacgg ggagacagga gaaauugucu gggacaaggg ccgggauuuc     3240

gcuaccguga gaaaaguucu gucuaugccc caggugaaua ucgucaagaa aacagaggug     3300

caaaccgggg gauuuagcaa ggaguccauc cugccuaaac gcaacagcga caaacuuauu     3360

gcccggaaga aggauuggga ccccaagaaa uauggcgggu ucgacucucc gaccguggcc     3420

uacuccgucc ugguuguggc uaagguggaa aaaggcaaga gcaaaaagcu caaguccguc     3480

aaagagcugc ucggaaucac aauuauggaa agaucuagcu uugagaagaa uccuaucgau     3540

uuccuggagg ccaaaggcua caaggaagug cgcaaagacc uuauuguuaa gcugcccaag     3600

uauucccugu uugagcucga aaacgggcgg aaaagaaugc uggcgucugc uggagagcuu     3660

cagaagggca augaacuggc ccucccuagc aaauacguga acuuccugua ucucgccucc     3720

cacuacgaga agcugaaagg gucucccgag gauaaugaac agaagcagcu uuucgucgag     3780

caacauaagc acuaucugga cgaaaucauc gagcagauua gcgaauuuuc caaacgggug     3840

auccuggcug augccaaccu cgacaagguu cugagcgcgu acaacaaaca ucgcgauaag     3900

ccuauuagag agcaggcuga gaauaucauu caccuuuuca cgcugaccaa ccucggagcc     3960

cccgccgcuu uuaaauauuu cgacacaacc aucgaucgga agcgcuacac aucuaccaaa     4020

gaagugcugg acgccacguu uauucaccaa uccaucacag gccucuacga gaccagaauc     4080

gaucugagcc agcuuggggg cgacuga                                         4107


<210>  4277
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4277
augagcagac ccuacaacau cggccuggac auugggaccu ccucuaucgg auggagcgug       60

gucgaugacc aguccaagcu cguuucugug cggggcaaau auggguacgg agugcgccug      120

uaugaugagg gccaaacagc cgcugaaaga cggagcuucc gcacgaccag acggcgccuu      180

aagagacgga aauggcgccu ggggcuccug agagagauuu uugaaccuua caucacaccg      240

auugacgaua ccuucuuucu ucggcagaag caguccaauc ugucucccaa agaccagcgc      300

aagcucuaua cacaaaccag ccuguucaac gauagaacag accgggccuu uuacgaugac      360

uauccuacca ucuaccaucu gcgcuacaga cucaugacgg agaagcggca guucgauauc      420

agagaaauuu aucuggcgau gcaccacauc gucaaauacc gcggacauuu ucuuaaugag      480

gcucccgugu ccagcuucaa gucuuccgag auuaaccugg uugcccacuu ugaccggcuc      540

aauaccaucu ucgccgaucu guuuagcgaa ucuggcuucc agcuugagac agacaaacug      600

gcugaaguga aggcccuccu gcuggacaac cagcaauccg cuagcaauag acagcgccag      660

gcccucucuc ugauuuauac cccuuccaca aacaaagcgg ucgagaagca gaauaaggcc      720

aucgcuaccg agcuucugaa agccauucuc ggccugaagg cuaaauuuaa cgugcuuacg      780

gggaucgaag ccgaggaugu uaaggcgugg acacugaccu ucaaugccga aaacuucgac      840

gaggaaaugg ugaaacucga gagcucccug gaugacaaug cucaucaaau cauugagucu      900

cugcaggaac ucuacagcgg aguccugcuu gccggcaucg ugcccgagaa ccagucccug      960

agccaagcua ugauuacaaa guaugaugac caccagaaac accucaagau gcugaaggcc     1020

gugcgggaag cccuugcucc uggggauaga cagcgccuga aacaggcgua cgaccaauau     1080

gucgauggac aggagaauac caaggccuac ucuaaagaag acuuuuacgg cgauaucacc     1140

aaggcucuca aaaacaaccc ggaccauccc auuguuuccg agaucaagaa gcugaucgag     1200

cuggaccagu ucaugccuaa acagcggaca aaggauaaug gggccauucc ccaccaacuc     1260

caucagcagg aacuggacag aaucauugag aaccagcaac aguauuaccc uuggcuugcc     1320

gaacugaauc ccaacagcaa acgccagacg guggcuaagu auaaacucga ugagcuggug     1380

gccuuucggg ucccguacua ugugggaccu cugaucaccg cggaacagca aagacagucu     1440

agcgacgcua aguucgccug gcuuauucgc aaggccgagg gccggaucac acccuggaau     1500

uuugaugaca aaguugauag acaggcuucc gccaacgagu ucaucaagcg gaugaccaca     1560

accgacacgu accuccuggc ugaagaugug cugccuaaac aaucucucau uuaucagcgc     1620

uuugaggucc ugaaugaacu uaacggccug aagaucgacg aucagcccau uacaaccgag     1680

cucaaacagg ccaucuucac cgaccuguuu augcaaaaga uuagcgugac aguuaagaau     1740

auccaggauu accugguguc cgagaaaaga uacgcgagcc ggcccgccau uaccgggcuu     1800

ucugacgaaa acaaguucaa uuccagacuc agcacauauc acgaccugaa aaugaucguc     1860

ggagaugcug uggacgaugu cgacaagcag gccgaucugg agaaaugcau cgaauggucu     1920

acgauuuuug aggacggcaa gaucuacucc gcuaaacuca acgaaauuga uuggcugacc     1980

gaccagcaac gcgugcagcu ugccgcgaag cgguauagag gguggggacg ccugagcgcc     2040

aagcuccuga cacagaucgu uaaugcuaac ggccagagaa uuauggaucu gcuuugggac     2100

accacagaua auuucaugcg gaucgugcac uccgaggacu uugauaaacu caucaccgag     2160

gccaaccaaa ugaugcuggc ugaaaacgac guccaggacg ugauuaauga ucuguacacg     2220

ucuccucaga acaagaaagc ccuccgccaa auccugcuug uugugaauga cauucagaag     2280

gccaugaaag ggcaggcucc ggagagaauc cugauugagu ucgcgcggga ggaugaaguc     2340

aacagccgcc ucuccgugca gagaaagcgg caaguggagc agguuuauca gaauaucucu     2400

aacgagcugc ugaauaacac cgaaauccgc aaugagcuua aggaccucag caacuccgcc     2460

cugagcaaua caagacuguu ccucuacuuu augcagggag gccgggauau guauaccggc     2520

gacucucuga acauugaucg ccuuuccaca uacgacaucg aucauauucu gccccagagc     2580

uucaucaaag acaauucucu cgauaacaga guccuggugu cccaaaagau gaaucggagc     2640

aaagcugacc aggugccuac cgacuuuacg ucugucgaac uggggcagaa gaugcaaauu     2700

cagugggagc agaugcuuag agccggacug aucacaaaga agaaguacga uaaccucacc     2760

cugaaccccg accacaucag caaauaugcc augaagggcu ucauuaaucg ccagcucgug     2820

gaaacacggc aaguuaucaa acuggcuacc aaccuucuga uggagcagua cggggaggau     2880

aauauugaac ucaucaccgu gaaguccgga cugacacauc agaugagaac ggaguuugac     2940

uucccuaaaa accgcaaucu gaacaaucac caucacgccu uugaugcgua ucuuaccgcu     3000

uucgucggcc uguaccuccu gaagcgguau cccaagcuca aaccguacuu uguguauggg     3060

gaauaccaaa aggccagcca gcaggacaaa uggagaaacu ucaauuuucu gaacggccuu     3120

aagaaagaug agcugguuga cgagaauaca gaagccguga uuugggauaa ggagucugga     3180

cucgcuuacc ugaacaaaau cuaucaguuc aagaagauuc uggucacccg cggcgugcac     3240

gaaaauuccg gggcccuuuu uaaccaaaca cuguacgcgg cuaaagacga uaaggccagc     3300

ggacagggcg ggaaacagcu caucccugcc aagcaggacc gguccaccgc ucuguaugga     3360

ggcuacucug ggaaaacggu ugccuauaug uguaucguga gaauuaagaa uaagaaaggc     3420

gaucucuaca aggucugcgg aguggagaca agcuggcugg cucaacuuaa acagcugacc     3480

gacgaagacu cccagaaggc cuuccuggag cagaaaaucu cuccccaauu caccaaggug     3540

aagaaacaga agggcaacau uaucgaggcg gucgaagagu uugaaguuau ugccccucau     3600

auccucauca accagcgcuu cuuugauaau gggcaagagc ugacacuugg aagcgcuacc     3660

uauaaacaca acgaacagga gcugauucuc gacaagacag ccgugaaacu gcucaauggc     3720

gcucugcccc uuacgcaguc cgaggaacug gccgaacagg uguacgauga aauccuggac     3780

caagucaugc auuacuuccc ucucuaugau accaaccagu uucgggccaa gcugucuaca     3840

gggaaggcug cguucaugaa acuucccugg aaaagccagu gggacggaaa uaaaauggug     3900

cagguuggcc aacaggugau ucuggauaga auccucauug ggcugcacgc caacgcugcc     3960

auguccgacc ucaagguccu gaaaaugacc acaccguuug gcaugaucca gcagagcacc     4020

ggaaucacgc uuucugccga uacccaaauu aucuaccagu ccccuacagg ccuguucgag     4080

cggcgcgugg cucugagaga ccucuga                                         4107


<210>  4278
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4278
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug auuccggaga gaccgccgaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuugc      240

uaucugcagg agaucuucuc uaacgaaaug gccaaagugg acgauagcuu uuuccauaga      300

cuugaggaau ccuuucuggu cgaggaggac aagaaacacg aacggcaucc uaucuucggc      360

aauauugugg augagguugc cuaccacgaa aaguauccga ccaucuacca ucuccgcaag      420

aaacuggugg acucuacaga uaaggcugac cugagacuca uuuaucuggc ccuugcucac      480

augaucaaau uucgggggca cuuccugauu gagggagauc ucaaccccga caauagcgac      540

gucgauaagc uguuuaucca acuugugcag accuacaacc agcuguucga ggaaaauccu      600

auuaacgccu ccggcguuga cgcgaaagcc auccucagcg cucgccuguc uaaguccaga      660

cggcuggaga aucucaucgc ccagcugccc ggcgaaaaga agaacgggcu uuuuggaaau      720

cugauugcuc ucagccuggg ccuuacgccu aacuucaagu cuaauuuuga ucuggccgag      780

gacgcgaaac uccaacuguc caaggauacc uacgacgaug accuggauaa ccuccuggcc      840

cagaucgggg accaguaugc ugaucuuuuc cuggccgcua aaaaccucag cgacgccauu      900

cugcuuucug acauccugcg ccucaauucc gaaauuacaa aggcuccccu gagcgcuucc      960

augaucaaaa gauacgauga gcaucaccag gaccugaccc uccugaaggc gcuugugcgg     1020

caacagcugc cugagaagua uaaagaaauc uucuuugauc agucuaagaa cggauacgcc     1080

ggcuauauug acgggggagc uagccaggag gaauuuuaca aauucaucaa gccgauucuc     1140

gagaaaaugg auggcacaga ggaacugcug gccaagcuga auagagagga ccuccugcgc     1200

aagcaacgga ccuucgauaa cggcuccauc ccccaucaga uucaccuggg ggaacuccac     1260

gccauccuga gacgccagga ggacuuuuau ccuuuccuua aagauaaucg ggaaaagauc     1320

gagaaaauuc ugacguuuag aauccccuac uacgucggac cucucgcucg cggcaacagc     1380

cgguucgccu ggaugacaag aaagucugaa gagaccauua cacccuggaa uuuugaggaa     1440

gugguggaca aaggggcguc cgcucaaucu uucaucgagc gcaugaccaa cuuugauaag     1500

aaucugccga acgaaaaggu ccugccuaaa cauagccuuc ucuaugagua cuucaccguu     1560

uauaaugagc ugacaaaggu gaaauacgug acggaaggaa ugcggaagcc cgccuuucug     1620

agcggcgagc agaaaaaggc cauugucgac cuccuguuca aaaccaacag aaaggugaca     1680

guuaagcagc uuaaagaaga uuauuucaag aaaaucgagu guuuugacuc cguggaaauu     1740

ucuggggucg aggaccgcuu caaugcuagc cugggaaccu accacgaucu ccugaagauc     1800

aucaaagaca aggauuuucu ggacaacgag gaaaaugagg auauucuuga agacaucgug     1860

cucacacuga cccuguucga ggaucgggaa augauugagg agagacucaa gacguaugcc     1920

caucuguuug acgauaaagu uaugaagcag cuuaaacggc gcagauacac aggcuggggc     1980

cggcugucca gaaagcucau caacgggauu cgcgacaaac aaagcggaaa gaccauccug     2040

gauuuccuga agucugacgg cuuugcuaac cggaauuuca ugcagcuuau ccacgaugac     2100

ucccucaccu uuaaagaaga cauucagaag gcccagguga gcgggcaagg agauucucug     2160

caugagcaca ucgcgaaccu ggccggcucc ccugcuauua aaaaggggau ccuccagaca     2220

gucaaagugg ucgacgaacu ggugaagguu augggcagac acaagcccga gaauauugug     2280

aucgaaaugg cccgcgagaa ccagaccaca cagaaaggac aaaagaauag cagagagcgg     2340

augaaacgca ucgaagaggg cauuaaggaa cuuggguccc agauccugaa agagcauccc     2400

gucgagaaca cgcagcucca gaaugaaaag cuguaccugu auuaccuuca aaacggaaga     2460

gauauguaug uggaccagga gcuggauauu aaucggcucu cugacuacga uguugaccac     2520

aucgugccuc agagcuucau uaaagaugac uccaucgaua acaagguccu gacccgcucu     2580

gacaagaaua gaggcaaaag cgacaacgug ccguccgaag aggugguuaa gaaaaugaag     2640

aauuauuggc ggcaacuccu gaacgcuaaa cuuaucacac agcgcaaguu ugauaaucug     2700

accaaggccg aaagaggggg acucagcgag cuggacaaag cgggcuucau uaagcggcag     2760

cuggucgaga cacgccagau caccaaacau guggcccaaa uucuugauuc uagaaugaac     2820

acgaaguacg acgaaaacga uaaacugauc cgggagguga aggucauuac ccucaagucc     2880

aaacugguga gcgacuuuag aaaggauuuc caguucuaua aaguucgcga aaucaauaac     2940

uaccaccacg cucaugacgc cuaccucaau gcuguggucg ggacagcccu gauuaagaaa     3000

uaucccaaac uugagucuga auuuguguac ggcgauuaua agguuuacga cgugcggaaa     3060

augaucgcca aguccgagca ggagaucgga aaagcuaccg cgaaguauuu cuuuuacagc     3120

aacauuauga auuucuuuaa aacagaaauc acccuggcca acggcgagau uagaaagcgc     3180

ccucucaucg aaacgaaugg ggagacagga gaaauugucu gggauaaggg ccgggacuuc     3240

gcuaccguga gaaaaguucu gucuaugccc caggugaaca ucgucaagaa aacagaggug     3300

caaaccgggg gauuuagcaa ggaguccauc cugccuaaac gcaauagcga uaaacuuauu     3360

gcccggaaga aggacuggga ccccaagaaa uauggcgggu ucgauucucc gaccguggcc     3420

uacuccgucc ugguuguggc uaagguggaa aaaggcaaga gcaaaaagcu caaguccguc     3480

aaagagcugc ucggacugac aaucauggaa agaucuagcu uugagaagaa cccuauugac     3540

uuccuugagg ccaaaggcua caaggaagug aaaaaggauc ugaucauuaa gcugcccaaa     3600

uauucccucu uugagcugga aaaugggcgc aagcggaugc uugcgucugc uggagagcug     3660

cagaaaggca acgaacucgc ccugccuagc aaguacguua auuuccucua ucuggccucc     3720

cacuacgaga aacuuaaggg gucucccgag gacaacgaac agaagcagcu guucguggag     3780

caacauaaac acuaucugga ugaaaucauc gagcagauua gcgaauuuuc caagagaguc     3840

auccucgcug acgccaaucu ggauaaagug cuuagcgcgu acaacaagca ucgggacaaa     3900

ccuauucgcg agcaggcuga gaacaucauu caccuguuca cgcucaccaa ucugggagcc     3960

cccgccgcuu uuaaguauuu cgauacaacc aucgacagaa aacgguacac aucuaccaag     4020

gaaguucucg augccacgcu gauucaccaa uccaucacag gccuuuacga gacccgcauc     4080

gaccugagcc agcuuggggg cgauuga                                         4107


<210>  4279
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4279
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa cagucaacga gaaauccucu aucguuaagc ugggcgugcg ggugaauccc      120

cucacggucg augaacagaa aaacuucgag gccggaaaga gcauuccuac caaugcggga      180

cgcacacuga aaagaggcau gcggcgcaac cuucaaagau acaagcugcg gcgcgacuau      240

cucauccagu gccugaagga auacgggugg auuaccuccg auacacugcu cuaugagugu      300

ggaaauagaa ccacauuuga gaccuaccgg cugcgcgcua aagccgugga cgaagagauc      360

gcccuugaag aacugggcag aguucuccug augaucaaca agaaacgggg cuauaagucu      420

aaucgcaaag cuaagagcuc cgaugagggg cagcuuauug acggaauggc caucgcuaug      480

gauauguacc acaacggcac gaccccgggg gaauaugugu acucucugcu cagcaagggg      540

aaaaaguaca uucccgucuu cuauagaucc gaccugcugg augaguuuga ccggaucugg      600

gauuaccagu gcgccuauua cccugacauu cucacaagcc aauucaaaga acugauccgc      660

ggcaagucuu cccaggugac cagcaaacuu uuuaagcagc aguauaaucu guucacagcg      720

gauaacaaag agaaggacaa gagacuccaa gccuacuguc ggagagcuga cgcccugaaa      780

aagcgccuuc ccguugaaga gguggcugcc gcgcugugcg augucaaucg ggacgugaac      840

gccucuuccg gguaucucgg aaccauuagc gauagaucua aaaagcuguu uuucaauaaa      900

cagacggugg gccaguaccu gaugcucgag cuggacaagg auucccauuu uagcgucaag      960

aacgaaguuu ucuaccgcca ggacuaucuu aacgaguuug aagcuaucug ggagaaacaa     1020

gccgaauacc acccugagcu gacacccgau cggaagaaaa uccucagaga cguggugauu     1080

uucuaucagc gcccucugaa gucccagaaa gaucuugucg cuuguugcga gcuggaacgg     1140

agacgcaucg aggugaugaa ggacggcaag guucggucug uggaaauugg gccgaaaguc     1200

ugucccaaga gcuccccucu cuuucaagau uucaaaaucu ggcaugugcu gaauaaccug     1260

aagguuagag acaaagccac cggagacgag cgcuuccucg aacuggauga gaaggagauu     1320

cuuuuuagcg aacuggccau caagucuaaa cucuccaaga cagaggcucu gaaacugcuu     1380

uaccagaagc ccaaaggccu cgaccugaau uaugauagcg uggaagggaa caccacccag     1440

gcggcccugu ucgaggcuua ccagacaguc aucgccauga cgggacacgg cgaguaugug     1500

uuuucuaaga ugagcgccuc cgaagucaug ucugugguug aggggguguu cggaggccuc     1560

ggcuuuaaua ccagcguccu gcgguucgac ucccuucugc aagggaaaga acucgagaag     1620

caggauagcu acagacugug gcaucugcuu uacucuuaug aaggagacaa cuccgugaca     1680

ggcaccgaug ggcucguugc uaagguggcc agccugcugc ggugcgacga ggaguacgcg     1740

aaagucgugg cuggagugac auuucagccu gauuauggcu cucucuccgc caaggccauu     1800

cgcagaaucc ugccccacau gcgggcuggg aaugaauaca gccuugccug ugaguaugcu     1860

ggcuacaaac acuccagacg cucucugacc aaggaggagc ucgaacagaa agacuauaag     1920

gaucaucugg agcugcuucc gcggaacagc cucagaaauc cuguugucga gaagauucug     1980

aaccaaaugg ugaauguggu caacgccgug guugacgcgu acggaaaacc cgaugaaauc     2040

cgcauugaga uggccagaga acugaagaaa uccgcuaagg agcgggaaga gaugacggcc     2100

gcuaucaaua aagccacauc ugagcacgaa cgcaucaagg agauucucaa caaggaauuu     2160

ggcauccagc augugagcca caaugacauu gucagauaca aacuguauug cgagcuuaag     2220

aacaacgggu acaaaacccu guauuccgac accuacaucc cucaggagaa gcucuuuagc     2280

aaagaagugg auauugagca cauccugccc caggcgcggc uguucgacga uucuuuuucc     2340

aauaagacac uugaauaucg cagcguuaac auugagaagu cuaaucaaac cgccugggac     2400

uacguggcug ccaaaugggg agaagagggc ucccucagau auaagaaacg ggucgaugac     2460

cugcuggcug ccggggugau cggaaagagc aaacaugaua agcuccugau gaaacaggag     2520

gacaucccca cagauuucau uaaccgcgac cuuagagaua cgcaguacau cgcccggaag     2580

gcucgcgaaa uucuggagga ccucguuccu augguggucu cuaccacagg cgcggugacc     2640

gauagacugc gggaagacug gcagcuggug gacgucaugc aagagcuuaa uugggauaag     2700

uacgccauga aagggcugac agaagagugg accgacaagg auggcagacg caucagccgg     2760

auuaaagagu ggacgaagag aaacgaccac cgccaucacg cuauggaugc ccucaccauc     2820

gccuuuacaa aacauuccuu cauccaguau cugaauaacc ucaaugcucg gagcgacaag     2880

ucuggaucca uuuacgccau cgaacagaag gagcuguaua gagauaacca cggcaaacuu     2940

cgcuucauua ugccguuuga aggguuccgg agcgaggcga agagacaacu ggaaaauauc     3000

cucguuucca uuaaagcuaa gaacaaagug gugacccgca augucaacaa ugcccggaga     3060

ggaucuggcc ggcaggccaa ggugcagcug acaccccgcg ggcagcugca caacgagacc     3120

aucuacggaa gcauccauag auauguuacg aaacuugaga aggugggcgg gaacuuugac     3180

ggcgaacaaa uugcuaaggu cgccaaucug cgguaccgcg aggcgcucca ggcuagacac     3240

gaagccuucg gaggcgaugc caaaaaggcu uuuacaggga aaaacgcccu ggacaagaau     3300

ccucucuauc ugaaggauac ccggacagag gugccccuua aaguuaagac cguguccacc     3360

gagacacugu acacgaucag aaaagcuguc gccccugacc ucaacgugga caagguuauu     3420

gaugcgaaag ugcgcaaggu ccuggaggaa cggcugcagg aauacggagg cgaccccaag     3480

aaagccuucg ugaaucuuga ugagaacccg aucuaucuga auaaggcuaa agggauuacc     3540

aucaagagag uggccaucac aggagucaac aaugcuauug cccuccauga caaaaaggau     3600

guuggcgggc acgugaugcu ggacgccucu ggccgcaaac agccugugga uuuugucaac     3660

accggaaaua accaccaugu ggcuaucuac cgggacgcgg aaggcagccu ccaagagguu     3720

guggucuccu ucuaugaggc cguggcuaga guuucucugg ggcuucccgu gauugaucgc     3780

gaauaccgga aggaggacgg auggcaguuu cuguucacaa ugaagcagaa ugaauauuuu     3840

gucuucccua accccgagac cggcuuuaau ccucaggaag uggaucugac ggacgagaca     3900

aacuacgccc ucaucagccc caaccuguuc agaguccaaa aauucaccau uaaggauuau     3960

uucuuccgcc accaucuuga gaccacagug gaagacagaa aagagcugaa ggacaugacc     4020

uggaaacggu gugggcucgg aggcaucauu ggcaucguua aggugcgcgu caaucacauc     4080

gggagaauug uguccguugg agaauacuga                                      4110


<210>  4280
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4280
auggccaaga ccuacaacau cggccuggac auugggacaa auagcaucgg augggcugug       60

gucgaugacc agaaccaccu cauguccauu aaaggcaagu auggguacgg aguuagacug      120

uaugaugagg gcaaaacggc cgaagaacgg cgcucuuuca gaaccacacg gcgcagacuu      180

aagcggcgca aauggagacu gagccuccug cgggaaaucu uugagcccca aauuaccccu      240

cuugacaaua cauucuuucu gcgccagaag caguccucuc ucagcuccaa ggauaacaga      300

aaacuguacc cggaaauguc ucuguucaau gaccggaccg auacagacuu uuauacccag      360

uaccccacga ucuaucaucu cagccgcgau cugaugacca agaacaaaca auucgacauc      420

agagagauuu accuugcgau guaccacauc gugaaguauc gggggcauuu ucugaaugag      480

uccccuguga gcucuuucaa auccagcgaa auuaagcucg cugagaacuu ugaaacacug      540

aaucagaucu ucaacgagcg cuuuaaugag ucuauguucc agcuugccgu caacgauuuu      600

gacgaucuga aggcccuccu gcugaaugac cagcaauccg cuagcaacag acagaaaaag      660

gcccucggaa ugauuuacgu gcccaaugaa aacaaaucuc uggagaagca gaacaaagcu      720

aucgccaccg aacuucugaa ggcgauucuc ggccugaaag ccaaguucaa uguucuuaca      780

ggcaucgagg uggacgaugc uaagccuugg acccuguccu ucaacagcga auccuuugac      840

gaggauaucg acaaagccgc uacgaauauu gaugacgauu cucgggagau cauugccauc      900

cuccaggggc uguauagcgg gauucugcuc gcgggcauca uccccgaaaa ccaaucccug      960

agcgaggcca uggucgaaaa guacgaccag cacgcugccc aucuuaaaca gcugaagucu     1020

auuuauccuc uccuguccau cagcgauaaa aaggcuauuc aacaggccua caauuauuac     1080

aucgacgggc aggauaacgc caaggcuuac ucuagagagg acuucuaugc ggccacaacc     1140

aaagcucuua agagcuacca ggacaaugaa acaauugccg agauccugaa acucaucgag     1200

cuggaacaau uuaugccgaa gcagcgcacc aaagauaacg gagccauucc ccaccagcug     1260

caccagcaag agcucgaacg gaucauugag aaucagcaga aguauuaccc uuggcuggcu     1320

gaacuuaacc ccaaugaguc cagacgcaag aucgccaaau auaagcugga cgagcucgug     1380

gcguuccggg uuccuuacua ugugggcccc cugauuaccu cugaacagca acagcagagc     1440

uccaacaaaa aguuugcuug gaugaucaga aaagccgagg ggaagauuac accguggaau     1500

uucgaugaaa aggucgaccg ccaggccagc gcuaacgagu uuaucaaacg gaugacgacc     1560

acagauaccu accugcuugc cgaggacgug cucccuaagc aaucucuggu guaccagaga     1620

uucgaagucc ugaaugagcu caacggaauc aaaauugaug acguucccau cacaguggaa     1680

cagaagcaac gcauuuuuaa cgaucuguuc augaugcagg cuuccgugag cgucaaaaau     1740

auccaggacu aucuuguguc uaagggcuac uuugccuccc ggccugagau uaccggccug     1800

agcgaugaaa acaaaguuau cuccagacuc ucuacguauc augaccugaa ggcgauccuu     1860

gggggaauug uggaugaggc caauaagcag gcugaccuug agaaaugcgu cgaauggagc     1920

acaaucuucg aggacggcaa gauuuuugug gaaaaacuca acgagaucga auggcugacc     1980

gaugagcaac gcaagcggcu guccucuaaa agauaccggg gguggggacg ccucagcaau     2040

aagcugcuua accagauugu uaaugagaac ggccagagaa ucauggaccu gcucugggau     2100

accacagaca auuucaugcg gaucgugcac uccgaagagu ucgauaagcu gauuagcgcc     2160

gcuaaccaga cccugcuuuc uaaaaaugac guccaagaug ugaucaacga ccucuauaca     2220

agcccccaga auaagaaagc ccugagacag auucugcucg ucguguccga uauccagaag     2280

gcgaugaaaa accaagcccc cucuaagauu cugaucgaau uugcucgcga ggacgaagcc     2340

aauccucggc uuuccguuca gagaaagcgc cagguggagc aagucuacca gaacaucagc     2400

aaagauauuc ugucugacag cgaugugaag gcugagcuca aagaccugac gaacaccgcc     2460

cuggccaaua caagacuuuu ccucuauuuu augcagggag gacaugacau guacaccggc     2520

gaaacacuga acaucgaucg gcuguccgag uacgacauug aucacauccu cccgcaggcu     2580

uucauuaagg acaauagccu ggauaaccgc guucuugugu cugcgaaaau gaauagaucc     2640

aagagcgacc aagucccccu gaacgauuau accgaggagc gguuuggcuc caagaugcgc     2700

gugcaguggg agcagaugcu cagagccggg cugaucacga aacggaagua cgacaaucug     2760

acccuuaacc cugaucgcau uucuaaauau gcuaugaagg gauucaucaa uagacagcug     2820

guggaaacac ggcaaguuau caaacucguc accaaccugc ucauggacca cuacgaggau     2880

gacgaagugc agcugauuac agucaagagc ggccuuaucc aucagaugcg cgagauguuu     2940

gacuucccca aaaauagaaa ccugaauaac caccaucacg ccuuugaugc cuaucucacc     3000

gcuuucgugg ggauuuaccu gcugaagcgg uauccuaagc ugacgcccuu uuucguguac     3060

ggauccuacc agaaaaauaa cacaaacgac aaguggagaa auuuuaacuu ccugaaugau     3120

cucaaaaagg aagagguuau cgacucugau agccaugagg ugauuuggaa caaagcccaa     3180

ggccuggucg aacucgacaa gaucuaucag uucaagaaaa uccuggugac ccgcgagguu     3240

cacgaaaaua caggggcgcu uuacaaccag acccuguauu ccgcuaagga ugacaaagcc     3300

ucuggccagg gaggcaagca acucauuccg gccaaacagg aucggccuac cgcucuguac     3360

gggggauaua gcggcaagac aguggccuac auguguaucg ucagaauuaa guccaaaaag     3420

gaggacacgu auaaagugug cgggguuaag accagcuggg uggcggaacu ggagcgccuu     3480

ucugauucca augagcagcu ggcuagccuc aaagccaagc ugaugcccaa guuuacaaag     3540

aacaagaaac aaaagggaaa aaccauccag gucacagaag acuucgagau uaucgugccu     3600

cgggugcuca ucaaucagag auuuuacgau aacggccagg aaauuacccu ggggucugcc     3660

acguacaagc acaaugagca agaacuuaug cugaacaaga aagcugucaa gcugcucaau     3720

ggccagcugu cccuuguuga ggccgagaca cuggcucccc agguguauga cgaaauccuc     3780

aaacagguga ugaaguacuu cccucuguau gacaccaacc aauuucgcac caaacucaag     3840

augggaaaag agcaguucau uaagcugccc uggaagaaua aaugggaugg caacaaacuu     3900

auccaugucg ggcagcagac agugcuggac cggauucuga ucggacucca cgccaaugcg     3960

gccguuagcg aucugucuac ccuuaaagug acaacgccgu uuggcaagcu gcaacagccu     4020

uccgggauug cucucagccu gaacaccaaa aucaucuacc agucccccac aggacucuuc     4080

gaaagacgcg ucgcccugcg gggccuuuga                                      4110


<210>  4281
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4281
auggacaagc ccuacaacau cggccuggau auugggagcc acuccaucgg auggaccgug       60

gucgacgaua auucuaaacu cguuagaauu aagggcaaac ugggguaugg agugcggcuu      120

uucgacgagg gcaagacagc cgaagaacgc agacaguuuc ggacgacccg cagacggcug      180

aaacgcagaa aguggcggcu ccgccugcuu agagaaaucu ucgagccuca uauugcuccg      240

cuggaugaaa cauuuuuccu ccggcaaaag cagagcaacc uggugcccac ccagaaaaag      300

cugccucagc ucucccuguu uaaugaccgc ucugaugccg aguucuacca caaauauccc      360

acaaucuacc aucuuagaaa cgcgcugaug accgagaagc ggcaauuuga ccuccgcgaa      420

aucuaucugg cuauucacca uaucgucaaa uacagagggc acuuccuuaa ugccgccccu      480

gcugccagcu uuaaguccgg aaaguucgau cuggagaaca aauuugacca gcucaaucug      540

cuguucgcuu cugccuauag cgaucucgac uuuacacugc ggaccgauaa cguggaccag      600

guuaaggcga uucuucugaa ugaagaccuc uccgccagca cgagacagcg ccaagcucug      660

ccccuuaucu accagaccuc ugccgaucag aaagcugcca agaaacugac aaagucccuc      720

gcgaccgagc ugcugaaagc cauucucggc cugaaggcua aguucgacgu gcuuacaggc      780

aucgagaccg augacacgaa agccuggcgg cugacauuca acgcugaaga uuuugacgau      840

aagcucaaug agcugaacgu caccgacaau gaagccgcca cacagauuau cgagauccuu      900

agagaacugu acagcucuau ucuccuggcu gcgaucgugc cugagaacua uacccugucc      960

caagccauga uggcuaaaua caaugcccac aagcagcauc ucagccugcu ucgccagguu     1020

aaaaccacac ugccggccaa gaagcaaaaa gagcuccagg cugccuauga ugacuacauu     1080

gaucagcauc ugccccugga caaguucuau gcgaaacuua cgaaggugcu cccuaaagaa     1140

aaggacaccg auaaccccca gaucauucaa auccugaacc ugaucgaccu cgaucaguuu     1200

cugccuaagc agcggacaaa agacaauggg gcuauucccc accagcuuca ccaacaggag     1260

cuggaucaga ucauugacaa ccagaaggcc uacuauccgu ggcucgccca acugaauccu     1320

aacgaaaaga gacagaagau cgcuaaauac aagcuggaug agcuugucgc cuuccgcgug     1380

cccuacuaug ugggaccucu cauugaagcu aagacccagc agaaaacauc uggcgccaau     1440

uuugcgugga ugauccggaa ggagcccggg accacgacac ccaucacccc uuggaacuuc     1500

gacgagaaag ucaauagaca agccuccgcu aacgaauuua uuaagcgcau gaccacaacc     1560

gauacauacc ugcugggaga ggacguucuc ccgaaacaca gccugcuuua ucagcgguuc     1620

gaagugcuga augagcucaa cggcaucaag auugaucaga aaagagugac gguccgccaa     1680

aagcaggcca ucuuuaauga ccuguuccug aagcagccca acgugaccgu uaaaaagcuc     1740

cagcaauacc uucaggcuaa aggggaauau gccuccgcgc cuauuaucac aggacugucu     1800

gaucccaaga aauuuaauag ccagcugucc accuaccaug aguauaagaa gauuuucggc     1860

ggccucaucg acgacccuga uaaacaggcc gacuuugaga agugcaucga auggagcaca     1920

auuuucgagg auucucaaau ccugaaagac aaacuuaaag aaauuaacug gcugaccccc     1980

gagcagcggc acaagguggc uaauaagaga uaccaggggu ggggacggcu cuccaaaaag     2040

cugcugacgc agcuugucga uuacaacggc caacgcauca uugacauccu cuggaacacc     2100

acagauaauu ucaugcagau cgugcauagc gaggccuuug acaaagcuau uaucaaggaa     2160

aaccaggccc ugcugaccca auauaauccg caggccguua uugaugaccu cuacacaucu     2220

ccucagaaca aaaaggcucu gagacagauc cuucuggugg ucaaugauau ucaaaaggcg     2280

cucaaagggc aggcccccag ccggauccug aucgaguucg cuagagaagg acaggagaac     2340

ccucgccugu ccacccagcg gaagcaacag guggaaaaug ccuauaaaga ggccucugac     2400

gcccuucuga gcuccgcuga ggucagacag gaacucaagg gccuggauaa caaagcgcuc     2460

gcuaauacgc gccuguuucu uuacuucaug caagggggaa gagacauuua uacaggcaag     2520

agccugaaca ucgacgaucu cucuaaauac gacauugauc acauccugcc ccaggccuuu     2580

auuaaggaca auucccugga uaaccgggug cuuguuagcg ccaagaugaa ucgcucuaaa     2640

uccgaccagg ugccucugaa cgauuucgcu gagaucagau auggcaagca gaugcggcag     2700

caaugggaga aaaugcuccg cgccgggcug aucaccagaa agaaauacca uaaucucaca     2760

cugaaccccg agagcauuaa caaguacacc gcgaagggau uuaucaaucg gcagcuuguc     2820

gaaacccgcc aggugacaaa acuggcuacg aaccuccugg uugaccaaua ugccgaggau     2880

gacgugcagc ugauuaccgu caaguccaau cuuacacacc agaugagaga ggaguucgau     2940

uuuccgaaaa accggaaugu gaacaaucac caucacgccu ucgacgcuua ccugaccgcc     3000

uuugugggcc ucuaucugcu caagcgcuac ccuaaacugg cucccuauuu cguuuacggg     3060

gacuaucaga agggagccuc ucaaaacagc aaguggagaa auuuuaacuu ccuuggcaau     3120

cugcaucggg agaaagucgu ggauaagaac acaggggaaa ucauuuggca gagagacgcg     3180

ggccugggag agcucaaucg caucuacaac uucaaaaaga uucuggugac ccgggcccuu     3240

agagucaauc gcggccccau guuuaaacag acgaucuaca aggcugaaga ggauaagucc     3300

ucugggcggg gaagcaaaca gcugaucccu acaaagaaaa acagaccgac cgagcucuau     3360

ggcggguaca ccaacaagga aacagccuau cugugucucg ugaaagagcc ccacaagaag     3420

ggagacauuu accgcaucug cuccguucug aauauguggc uuaccgaacu ggaucggauu     3480

gcugacccua ucgccaaagc ccaaaagcug acagagcucc ugacgcccca guucaccaca     3540

accaaaaaga aacagggcca gaagauuccu guggucaaac ccuuuaaggu gguuaucccu     3600

aagauccuua uuaaccaaag auucuuuagc cgcgggcagg agcugacagu ggcuucuccc     3660

auguauaugc acaaugaaca ggaacucuuc cugaacaaaa agaccaucaa acuccugaau     3720

ggccaacuug cgaagaacga agcccaugag cuggcuccga aaauuuacca gcagauccug     3780

gaucagguca augacuauuu uccucucuac gauauguaca aguuccggga cacgcugaac     3840

gccuccaagg aaauuuuugu gaaucuuccc uggcaagauc agagacagga caaaaagauc     3900

agccagucug gacaaaaauc cauccugaac aagauucuca ucggccugca cgccaaugcu     3960

gcccggauua aacucaccga gcugaacuuc aagagcgauu uugggcuucu gucucagcgc     4020

agcggaaucg cguucucccc ugacgaccag auuaucuauc agagccccac aggccuguuc     4080

gagagacggg uuacccucaa ggaucuguga                                      4110


<210>  4282
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4282
augaagguga acaauuacca caucggccug gacauuggga ccagcuccau cggauggguc       60

gccaugggcg aggaugggaa accccucaga guuaagggaa aaacagcuau uggcgcccgg      120

cuguuccgcg aagggaaccc ugcggcugac agacggaugu uucgcacgac cagacggcgc      180

cuuaauagac ggaaguggcg ccugaaacuc cuggaugaga ucuucgaccc guauauuaca      240

cccguggauu cuaccuuuuu cgccagacuu aagcagagca acuggucccc uaaggacucu      300

cggaaagccu uuaaggauag cacacuguuc cccgaccuca ccgauaaaca auaccaucug      360

gacuauccua caaucuacca ccuucgccau gcucucauga cccaggauaa gcaguuugac      420

aucagaaugg uguaucuggc ccuucaccau auugucaaau accggggaaa uuuccugaac      480

uccacgcccg uggauucuuu uaaggcuagc aagguuaauu ucgacgacca guuuaaaaag      540

cucaacgaac uguauaccgc caucaauccu gaggaauccu uccaaauuaa cuuugcgaau      600

agcgagaaaa ucggccagca guuccuugau ccgucuauuc gcaaguucga caaaaagaaa      660

cagaucucca agauugugcc cgucacaaug gaugacaagg ccguggauaa acugaacggc      720

aagaucgcua gcgagaucgu uaaugccauu cucggguaca aaucuaagcu ggacguggcu      780

guccaaugcg auccugaaga cuccaaaccc ugggccguga aguuugauga cgaggauauc      840

gacgcgaagc ugcagaaaau ucucccugaa auggacgaga accagcagag caucauugga      900

aucauccaag aucuguacuc ucaggugacc cuuaaucaga uuguccccaa cggcaugucc      960

cugagcgagu ccaugaucaa gaaauauaac gaccaccacg aucaucucaa gcuguacaaa     1020

aagcugauug ggcaaaucuc ugacagcaag aaaaagacag cccugaaaaa ggcuuaugau     1080

caguacguug gaaaugacgg caaagugauu gaacaggaug acuucuauuc cagcgugaag     1140

aagaaccucg augacucuga gcuguccaaa cagaucaugg aucugaucga cgccgauaag     1200

uuuaugccga aacaaagaac cagccagaau ggggucauuc cucaccagcu ccaucagcgg     1260

gaacuggacg agaucauuga acaccaaucu aaguacuauc ccuggcuugc ugagagcaac     1320

ccuaauaaac acgaccugca ucucgccaag uacaaaaucg aggaacuggu ggccuuccgc     1380

auucccuacu auguuggacc caugaucacg ccugaggauc agaagaaguc cggcgcugcg     1440

guguuuucuu ggaugaaaag aaaggaaccg ggccagauua cacccuggaa cuucgaccag     1500

aaagucgauc ggauggagag cgccaauaag uuuaucaaaa gaaugaccac aaaggacacc     1560

uaccuuaucg gggaagaugu gcugccugac gagucccucc uguaugagaa guucaaaguu     1620

cugaacgaac ucaauauggu gcgcgucaac ggagauagcc ugaagguggc ugacaaacaa     1680

gccauuuacc aggaucuuuu ugagaauuau aagcacaucu cugucaaaaa gaugcagaac     1740

uacauuaagg acaaaaccgg ccugcccaau aacccugaua ucuccgggcu cagcgacccc     1800

gaucauuuca auaacucucu gggaacauau aacgacuuua agaaacuguu cggcgacaag     1860

guggaugacc cggaucuuca ggacgauuuu gaaaagauug uugagugguc cacgguguuc     1920

gaagacaaga agauccuccg ggccaaacug aaugagauca gcuggcuguc cgauaagcaa     1980

aaagacgauc ucgagucuag cagauaccag cgcugggggc ggcuguccaa gaaacuucug     2040

accggaauug ucaacgacca gggcgaaaga aucauugaug agcucuggaa uacaaacaag     2100

aauuucaugc aaauccaguc ugacgacgau uuugcuaaac gcauucacga agccaacgcg     2160

gaccagauga aggcuaccga uguggaggac guucuggccg augccuacac aagcccucag     2220

aauaagaaag cuauccggca aguggucaag gugguggacg auauccagag agccaugggc     2280

gggguugcuc ccaaauucau uuccaucgag uuuacccgca gcgaagaccg gaacccuaga     2340

cggacgauuu cucgcgagag acagcuggaa gauacacuua aggacaccgc caaaacccuc     2400

gucaagucca ugaaucccga uauccugagc gagcuggaca acgcgucuaa guccaaaaag     2460

ggacucacag aucggcugua ucuuuacuuc acccagcugg gcagagacau guauacaggg     2520

aaaccuauua auaucgacga aaucagcaac uacgauauug accauauccu cccccaagcc     2580

uuuauuaagg augacucucu gagcaaucgc gugcuggugu ccaaagcugu caacaaugga     2640

aagagcgcca acgugccgcu uaaacucuuc ggcguuaaaa uggcucacuu uuggaagcag     2700

cuggccgagg cggggcugau caacaaacgg aagcucaaaa aucugcagac gaacccugau     2760

accauuucua aguucgccau gcacggcuuu aucagacgcc agcuugugga gacaucccaa     2820

gucauuaagc ugguggcuaa uauccucgga gacaaauaua gaaacgauga caccaagauc     2880

auugaaauca cagcccggau gaaucaucag augcgcgaug aguucgcuuu uauuaaaaac     2940

agagaaauca augacuacca ccaugccuuc gaugccuauc ugaccgcuuu ucugggccgg     3000

uaccuuuacc accgcuauau uaagcucaga cccuacuucg uuuaugggga cuucaaaaag     3060

uuucgggagg auaaggugac gaugcgcaac uucaauuuuc ugcacgaccu gaccgacgau     3120

acacaggcga gaaucgccga caccgaaaca ggagagguca ucugggaucg ggagaccagc     3180

auuaaacaac uccgcgacgu guaccauuau aaguucaugc ugaucuccca ggaaguuuac     3240

acgcuuagag gcgcuauguu uaaccagaca auuuaucccg ccgaugaggc cgggcggaaa     3300

aagcugaucc cuaccaaagc ugacaaggcc guggaugucu acggaggcua cucugggagc     3360

aaugacgcgu auauggcuau ugugagaauc cacgauaaaa agggcgacaa guacaaagug     3420

gucggaguuc cgaugcgcgc ccucgaucgg cugaacgccg cuaagaaugu guccgacgcc     3480

aaauaugacc aggugcugaa ggauguccuu acaccccaau ucaccaaauc uaaaaagagc     3540

agaaagaccg gcgaaaucuc ccagacagug gaggacuuug aaguugugcu ggggaagguc     3600

auguaccgcc agcucgugau ugauggagac aaaaaguuca ugcugggcuc uagcacguau     3660

caguacaacg cgaaacaacu cguucugucc gaugagagcg ugaagacccu ugcuucuaag     3720

gggaaacugg acgaggccca ggaauccgcc gcuuaugaua augucuacga cgagauccuc     3780

gauaaggugg accgguacuu ugcccuguau gaucagagaa acuaccgcac aaaccugcau     3840

gaaggagagc agaaauucau uggccuuccu cugcacgaca ccuuugaugg gagcaagaaa     3900

guugcuaaug gcaagcggga gacaaucucu gacauucuca acggacugca ugccaaugcg     3960

accaugacga accucaaggu gcugggcauc uccagccccu ucgggauguu ccaaucucac     4020

ggaccuauua aucuuaaccc cgaaacaauc auccuguguc aguccccuac cggccucuuu     4080

gagagacgca uuauccugcg ggaccuguga                                      4110


<210>  4283
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4283
augaagaaaa gaaacuacaa uaucggccug gacauuggga ccagcuccau cggauggaca       60

auuguggaug acaacgccca gaucaugcgg gcuaagggca aaaaugggua uggaguccgc      120

auuuucgccg agggcaagac ggcggcugau agacgggggu uucgcaccac aagacggcgc      180

cucaaaagac ggaaguggcg ccugagacuu cugcaagagu ucuuugaacc cuacauccuc      240

ccuaucgacg aaaacuucuu uaugcggcag aaggagucua aucugguucc gcaggauccc      300

aacaaacacu ucaagggaag cauucuuuuu aaugaccgca ccgaucagga guuccaugaa      360

caauauccua caaucuacca ccugagauau gcccucauga ccgagcaucg gcaguuugac      420

gugcgcgaaa uuuacugggc cauccaccau auugugaaau auagaggcca cuuccugaac      480

uccacacugg cuucuaaguu uaaaggcggg gagcuccggc uggccgauaa guucgaaagc      540

cuuaauaagc uguuucucag aguccaggcu gagaccggau ucgagcuguc cgaagacaac      600

guggauaaaa ucaagacggu ucuucuggac aauggcaaau cucgccucga ucggcagaga      660

gaggugcuga agauugucua ccugaccagc gaagacaaag acacagagaa gaaacgcaag      720

gccguggcga ccgaguucau caaggccguu cucgggcuga aaacaaaacu uuuuaucgug      780

cuggcuaaaa accccaccgc cgaaacggau uggaaguucu ccuuugacgc ugaggauauu      840

gacgauaaac ucgacagcau cucugaacaa cugacagaug acgaucacga gauugucgaa      900

cuuaucuccg agauucauag caucaucacc cugacaggag uggugccuga aggcaugggg      960

cucucugagu ccaugauugc caaguacgac cagcaccagc uggaucugaa gaaacucaag     1020

agccugaugc gggaccuuga cgucaaacag gcgagaaagc ugcaagaugc cuaugacgag     1080

uacaucgaug gacucaaacc cucugaagag gcuuucuauu ccgccguuag cuccgugauu     1140

cagaagaugg acgauccucg cgcuaagaaa aucaccgccg aaauugagcu ggaagaguuu     1200

augccgaagc agcggaccaa agccaauggc caaauccccc aucagcuuca ccagcaggag     1260

cuggaccaaa ucauugaaaa ccagaaggag uacuauccuu ggcucgcuga agagaauccc     1320

aacaaaagag agcacaacgc ggccccucau aagcuggaug gccuggugac auuccgcguc     1380

cccuacuaug uggggccgcu caucgaggcu ucuguucagc agaagacgag caauuccaac     1440

uuugccugga uggaacggaa agugaagaau gacaccagca gaauuacacc uuggaacuuc     1500

gaagagaaag ucgaucgcaa ugccucugcu gaaaacuuua ucaagcggau gaccacaaaa     1560

gacaccuacc ugcuuaauga ggaugugcug ccccaacagu cccuccugua ccagagauau     1620

gagguucuga acgaacuuaa uaacgugcgg gucaaugaca agaaacucga gaacaauugc     1680

aagcaggaug uguacaagga ccuguucaaa aacaauacga cagucagcau uaagaaauuu     1740

aaggacuauc ugguggccaa cugugaauac gcggauaacc cuaaaaucga gggacucgcu     1800

gacgaaucua uuuuccugag cucccuuucu accgagaaua aguuuaagga uaucuucggc     1860

gcccaaaucg acgaucccaa cuauuucgag gacuuugaac acauuaucga gugggccacc     1920

auuuucgaag auaauaaaau ccugcgcgcu aagcucgacg agauuagcug gcugaacgaa     1980

ucccagaaaa aggagcuggc caaacuuaga augcgggggu ggggaagacu cagccgcaag     2040

cugaucgcug agaucacaga ugaaaauggc ucucggauua ucgaccugcu cugggagcau     2100

auggauaacu uuaugguugu ggucaaucag gacguguuca aggaaaagau uguugccgag     2160

aacaaacagg uguuuaagga gcugaauccc cuugauauca uuaacgaaca auucaccucc     2220

ccucagaaua agaaagcgcu gagacagauc cuccuggucg uggccgacau ccaaaaggcu     2280

augaacgggg ugacaccgag caauauuuuu aucgaguucg cccgcgaaga cgagagaaac     2340

ccccggcgcu cuauucagag acugcggcag guugaaaaua uguaccgcga uaucuccgac     2400

acgguccuug cugauccuga ggugagaaac gagcucagca acauuaagaa uaccgaacug     2460

gcccgggacc gcguguuucu guauuuccuc cagggaggca gagaugucua cacaggcgag     2520

ccccugaaca ucgacaaacu uuccgaauac gauaucgacc acauucugcc ucaagcguuu     2580

aucaaggaua auucucucga caaccgggug cugguuagcg ccucccugaa ucgcucuaaa     2640

agcgauuccg ugccccuuga cgacuucacc agcgagaugu uugggagaaa gauggaaccg     2700

cucugggagc agaugcagcg ggcuggacug auuucuaaaa agaaacuggc caaccucaag     2760

uucaauccug agaacaucaa uaaguauaca agaaacggcu ucauuaaucg ccagcugguc     2820

gaaacccggc aagugaucaa acuugcugcc aacauucuga aggaucauua cggggaggaa     2880

acggagaucg uuaaugugaa agccggacuc acccaccagu uuagaaagga cuucgauuuu     2940

cccaaaaacc gcaaugucaa caacuaucac caugcuuucg acgcguaccu gacagcccug     3000

gugggcaccu aucuucugaa gaaguacccu aaacucgaau ccuauuuugu uuacgggaag     3060

uucaaaaagg gcgauggaau cacaaaucug aaaaacuuua auuuccucca cggcuuuaac     3120

aagcccggga agauuaucaa uaaagagacc ggagagauuc uguucgaccg ggauaagcag     3180

cuuggcuaca ugaacagcau cuaugaccug agaaaaguga uugucacgca ugaacucucu     3240

acacgccacg gggcuaugua cgaucagacc cuguauaagg ccuccgacga uaaagacagc     3300

ggacggggcg ggaagcaacu gaucagaaag aaagauaauc uuccuacaga gaucuacggc     3360

ggauauaccg gcucuaccga cgccuuuaug agcauuaucc gcaugaagaa aaaggacaaa     3420

cuguacuaua aggugauugg ggugcccaca cgggucgcuc acgaaguucu ccaguccgau     3480

ggaaagauca acauuuucac gcugaauaaa uucaucgagc agaagcucac caaagagaag     3540

gugaacaaaa agacaggcgc caucaccaga auuacagacu gguuugaacu ggugaucgcg     3600

aaagucaugu acgggcaagu gauugaugac ggaggccagc cguucaugcu ugggagcucu     3660

acccugccuu acaauguucg ggagcucuau cuggcuaagg aucuggugaa ggucaucaac     3720

aaaggcucca cgagcaaguc cucuuaccuu gacguguaug augaacuggu uaaaagccuc     3780

aauaaguacu uucccaugua ugaccgcaac auguucagac agaaaauuac agaugcccgg     3840

gacaaguuug agaagcugcc caaacgcgau aaggugucca auggaaaacu ccagcaaauc     3900

ggccagguca ccauuaucga cagaauccug auugggcuuc auaccaacgc cggagugucu     3960

aaucugaagg aucugggcau cgcuacaccu uucggguuua ugcagcaggg aaacggcauu     4020

agccucaccu ccacagccaa aaucauuuac caaucuagca cggggcuguu cgaaaagucc     4080

aaggcgcuug acgaccugaa cccgcucgcu uga                                  4113


<210>  4284
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4284
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaggu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcc cuucuguucg acggaggcaa caccgcugcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acaaugcaag aggaaaagua cuaucaugag aaauucccca ccaucuacca ucugcggaag      420

gaacuggcug acaagaaaga gaaggccgau cuccgccuga uuuaccuugc ucuggcccac      480

aucauuaaau uuagaggaca cuuccucauc gaggacgauc gguuugacgu ccgcaacaca      540

gacauucaga agcaguauca ggcguuccug gaaaucuuug auaccacguu cgagaauaac      600

gaccuucugu cccaaaaugu ggauguugaa gccauccuca ccgacaaaau uucuaagagc      660

gcuaaaaagg auagaauccu ggcccgguac ccuaaccaga aguccacagg cauuuuugcu      720

gaguuccuga aacucaucgu gggcaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacac cccuucaguu cgcgaaagac agcuaugaug aggaccugga gaaucuccug      840

gggcaaauug gagaugaauu ugccgaccuu uucucugcug ccaagaagcu guacgacucc      900

guccuccuga gcggcauccu gaccgugacc gaucucucua cgaaagcucc ucuguccgcc      960

agcaugaucc agagauauga cgagcaccgc gaagaucuua agcagcugaa acaguuuguu     1020

aaggccucuc uccccgagaa auaccaagaa auuuucgcug acuccagcaa ggauggguau     1080

gcgggauaca ucgagggcaa gacaaaccag gaggccuuuu acaaauaucu guccaaacuu     1140

cugaccaaac aggaaggguc ugaguaccuc cuggaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaaa gaacauuuga uaacggaagc aucccucacc aggugcaucu caccgaacug     1260

cgcgcuauua uccggagaca guccgaguau uacccguucc uuaaggagaa ccuggaccgc     1320

aucgaaaaga uucucaccuu ucggaucccc uauuacgucg gcccucuggc cagagaaaag     1380

agcgauuucg ccuggaugac acgcaaaacg gacgauucua uucggcccug gaauuuugaa     1440

gaccuggugg auaaggagaa auccgcugag gccuucaucc acagaaugac caacaaugac     1500

cuuuaucucc cugaagagaa ggugcugccc aaacauagcc ugauuuacga aaaguucaca     1560

gucuacaacg agcucaccaa gguucgcuuu cuggcggaag gcuucaaaga cuuucaguuc     1620

cuuaaucgga agcaaaaaga gacaaucuuu aacucucugu ucaaggagaa aagaaaggug     1680

accgaaaagg auauuaucag cuuucucaau aaaguggacg gguaugaggg aaucgcuauu     1740

aagggcaucg aaaaacaguu caacgccucc cugucuacgu accacgaucu gaagaaaauu     1800

cuugggaagg acuuucucga uaauacagac aacgagcuga uccuggaaga uauuguccag     1860

acccucaccc uguucgagga ccgggagaug auccgcaaga gacuugauau cuauaaagac     1920

uuuuucacag aaagccagcu gaagaaacuc uaccggagac acuauaccgg auggggccgc     1980

cuguccgcca agcugauuaa ugggauccgg aacaaagaga aucaaaagac aauucuugau     2040

uaccucaucg acgauggaag cgcuaacaga aauuuuaugc agcugauuaa ggacgccggc     2100

cugucuuuca aaccgaucau cgacaaggcu cgcacgggcu cccauagcga uaaccucaaa     2160

gaagugguug gggagcuggc cggaucuccu gcgauuaaga aaggcauccu ucagucccug     2220

aagauugugg acgaacucgu caaagugaug ggguaugagc ccgagcagau cguuguggaa     2280

auggccagag agaaccaaac cacagcuaag ggacugagcc ggcugcgcca gagacuuacc     2340

acacuccggg aaucccuggc caaucugaag ucuaacauuc ucgaggagaa aaagccuaaa     2400

uacgucaagg aucaggugga aaaucaccau cugagcgacg aucgccuuuu ccuguacuau     2460

cuccagaacg gcaaagacau guacaccgau gacgagcugg auaucgacaa ucugucccaa     2520

uaugauaucg accuuauuau cccccaggcu uuuauuaagg acgauucuau cgacaacaga     2580

guccugguga gcuccgccaa gaaucggggg aaaagcgaug acguucccuc uguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa cuccuggacg cgaagcucau gucccagcgc     2700

aaguacgaua accugacgaa agccgagaga ggcggacuua ccagcgacga uaaggcucgg     2760

uuuauccaac gccagcuggu ugaaacaaga cagauuacca aacacguggc ccggauccuc     2820

gacgagagau ucaauaacga gcuggauucu aagggccgcc ggaucagaaa agucaagauu     2880

gugacacuga aguccaaucu ugugagcaac uuucgcaaag aauuuggguu uuauaagauc     2940

cgggagguua auaacuacca ucacgcucac gacgccuauc ugaaugccgu cguggcuaaa     3000

gcgauucuca ccaaguaccc ucagcuggaa ccggaguucg uguacggaga uuaucccaaa     3060

uacaacucuu auaagacgag aaagagcgcc acagaaaaac ucuuuuucua cuccaacauc     3120

augaauuucu ucaagaccaa agucacacug gcugacggca ccgugguugu gaaggacgau     3180

auugagguca acaaugacac cggggagauc gugugggaua aaaagaaaca uuucgccaca     3240

guucgcaagg ugcuuagcua uccucaaguc aacaucguga agaaaacgga aauucagacc     3300

ggaggcuuuu cuaaggaguc cauccuggcc cacgggaaua gcgacaaacu cauuccccgg     3360

aagacaaaag auaucuaccu ggacccuaag aaguauggcg gauucgauuc ccccauuguu     3420

gcuuacucug ugcuggucgu ggccgacauc aaaaagggca aagcgcagaa acuuaagacc     3480

gugacagaac ugcucgggau caccauuaug gagagaagcc gcuuugaaaa gaacccgucc     3540

gcuuuccugg agucuaaggg auaccucaau auccgggaug acaaacugau gauucuuccu     3600

aaguauagcc uguuugagcu ggaaaacggc agacggcgcc uccuggccuc cgccgaugag     3660

cuucagaaag ggaaugaacu ggcucucccc acgcaauuca ugaaguuucu guaccucgcc     3720

ucuagauaua acgagcugaa aggaaagccu gaggaaaucg agcagaagca ggaguucguc     3780

guucagcaug ugagcuacuu ugacgauauu cuucaaauca uuaaugacuu cuccaaccgg     3840

gugauccugg cggacgcuaa ucuggagaaa aucaacaagc ucuaucagga uaauaaagaa     3900

aacauucccg ucgacgagcu ggccaacaau aucauuaacc uuuuuacauu caccagccug     3960

ggcgccccug cugccuuuaa guucuucgau aaaaucgugg accgcaagag auacaccucu     4020

acaaaagagg uucucaauuc cacccugauu caccagagca ucacagggcu cuaugaaacg     4080

cggaucgauc ugggcaaacu uggagaggac uga                                  4113


<210>  4285
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4285
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucaucacgg augacuauaa gguucccgcu aaaaagauga aagugcucgg caacaccgau      120

aagaaguaca uuaaaaagaa ucugcuuggg gcccuucucu ucgacucugg agagacagcg      180

gaagcuacca gacugaaacg gacagcccgc agacgguaua cccgcagaaa gaaccggcuu      240

cgcuaccugc aggagaucuu ugccgaagag augacaaaag uggaugagag cuucuuuuau      300

agacucgacg aguccuuccu gaccacggau gagaaggacu uugaacggca cccuauuuuc      360

ggcaauaagg cugaggaaga ugccuaccau caaaaauuuc cgaccaucua ucaucugcgc      420

aacuaccucg cugacucuag cgagaaggcc gaucugagac uugucuaccu ggcgcucgcc      480

cacaugauca aauaucgggg gcacuuccug auugagggaa agcugaaugc ugaaaacaca      540

gacgugcaga aacuguuuac cgauuucguu ggcguguacg accgcacauu ugacgauucc      600

caucucucug agaucaccgu cgacguggcc agcacgcuga cagaaaagau uuccaaaagc      660

agacggcugg agaaucucau caaguauuac cccaccgaaa agaaaaacac acuguucggc      720

aaucuuauug cucuggcccu cgggcugcag ccuaacuuua agaugaauuu caaacuuucu      780

gaggaugcga agcugcaguu cuccaaagac accuaugagg aagaucucga ggaacugcug      840

ggaaagaucg gcgacgauua cgccgaccuc uuuaccagcg cuaagaaccu guaugaugcc      900

auucuucugu cugggauccu cacaguugac gauaauucca cgaaagcucc ccugagcgcc      960

ucuaugauca agcgcuacgu ggagcaccac gaagaccuug agaaacugaa ggaguucauu     1020

aaagccaaca aguccgaacu cuaccaugac aucuuuaagg auaaaaacaa gaauggauau     1080

gcuggcuaca uugagaacgg ggucaaacaa gacgaguucu auaaguaccu gaaaaauacc     1140

cugagcaaga ucgauggauc cgacuauuuu cucgauaaga uugagagaga agacuuccug     1200

cggaaacaga gaacauuuga uaacggcucu aucccucacc agauccaucu ucaggagaug     1260

cacgcgauuc ugcgccggca aggcgacuac uaucccuucc ucaaggagaa acaggauaga     1320

aucgaaaaga uucugaccuu ucgcaucccu uacuacgugg ggccgcuggu gcggaaagac     1380

agcagauucg ccugggcuga guaucgcucc gaugaaaaga uuacacccug gaauuuugac     1440

aaagucaucg auaaggaaaa gagcgccgag aaauucauca cccggaugac gcuuaacgac     1500

cucuaccugc cugaagagaa gguucugccc aaacacucuc auguguauga aacauacgcc     1560

guguauaaug agcucaccaa gauuaaauac gucaacgaac agggaaagga guccuuuuuc     1620

gacagcaaua ugaagcagga gaucuucgau cacguguuua aagaaaacag aaagguuacc     1680

aaagagaagc ugcuuaauua ucugaacaaa gaguucccug aauaccgcau uaaggaccuc     1740

aucggccugg auaaggaaaa uaaaucuuuu aacgcuagcc uggggacaua ccaugaccuu     1800

aagaaaauuc ucgauaaggc cuuccuggac gauaaaguga augaggaggu caucgaagac     1860

auuaucaaga cccugacacu cuuugaggau aaggacauga uccacgaacg gcugcaaaaa     1920

uauuccgaua uuuucacggc gaaccagcuu aagaaacugg agagacgcca uuacaccgga     1980

uggggccggc ucucuuauaa gcugaucaac gggauuagaa auaaagaaaa caauaagaca     2040

auccuggacu accuuauuga ugacggaagc gcuaaccgga auuuuaugca gcucaucaac     2100

gacgauaccc ugcccuucaa acaaaucauu cagaaguccc aggugguugg cgacguggau     2160

gacaucgagg ccgucgugca cgaucugccg ggcagcccug ccauuaagaa agggauccuc     2220

cagucuguca agauugugga cgagcugguu aaagugaugg gagauaaucc cgacaacauc     2280

gucaucgaaa uggcucgcga gaaucaaaca accaacagag gccgguccca gagccagcag     2340

agacuuaaga aacugcaauc uucccucaag gaacugggga gcaauauucu gaacgaggag     2400

aagccuuccu auaucgaaga uaaaguggaa aauucucacc uucagaacga ccagcucuuu     2460

cuguacuaua uucagaaugg aaaggauaug uacacgggcg acgaacugga caucgaucau     2520

cucagcgacu acgauauuga ccacaucauc ccccaagccu ucauuaaaga ugacuccauc     2580

gauaaccgcg uucugaccuc uagcgcuaag aaccggggga aauccgacga ugugcccagc     2640

cuugacauug ucagagcccg caaggcggag ugggugagac uguauaaguc uggccucauc     2700

uccaaacgga aguuugauaa ucugacaaaa gccgaacgcg gaggccugac cgaggcugac     2760

aaggccgggu ucauuaaaag acagcuugug gagacacggc agaucaccaa gcauguugcu     2820

cagauucugg augcccgcuu uaacacggaa cacgacgaga augacaaggu caucagagau     2880

gugaaaguga ucacacucaa gagcaaccug gucucucaau uccggaaaga cuucgaauuu     2940

uacaaggugc gcgagauuaa ugauuaucac caugcgcacg acgccuaccu caacgcuguu     3000

gugggaaccg cccugcuuaa aaaguauccu aagcuggcuu ccgaguucgu cuacggcgaa     3060

uauaaaaagu acgauaucag aaaauuuauu acaaauagcu cugacaaggc caccgccaaa     3120

uacuucuuuu auagcaaccu caugaauuuc uuuaagacca aagugaagua cgcugauggg     3180

acaguuuucg agcggccgau cauugaaacg aacgcggacg gagagaucgc cuggaauaag     3240

cagaucgauu uugaaaaagu gcgcaagguc cuguccuauc cccaggugaa cauuguuaaa     3300

aagguggaga cccaaacagg cggguucagc aaagaaucua uccugccuaa gggcgacucc     3360

gauaaacuua uucccagaaa gaccaagaaa gucuacuggg acacaaagaa auauggaggc     3420

uuugauagcc cuaccguggc uuacuccgug uucgucguug ccgacgugga gaaggggaag     3480

gccaaaaagc ugaaaacggu gaaggagcuc gucggaaucu cuauuaugga acggagcuuc     3540

uuugaggaaa aucccgugga guuccuggag aacaaaggcu aucauaauau cagagaagac     3600

aagcucauca agcugccgaa auacucccuu uuugaguucg aagggggacg ccggagacug     3660

cucgcuucug ccagcgagcu gcagaagggc aacgaaaugg uucugccugg guaccuugug     3720

gagcugcucu aucacgcgca ucgcgcugau aacuuuaauu ccacagagua ccugaacuau     3780

gucucugaac acaaaaagga guucgaaaaa gugcucagcu gcguugagga cuuugccaau     3840

cuguacgugg augucgaaaa gaaccuuucc aaaauucggg ccguggcuga cagcauggau     3900

aauuucucua ucgaggagau uuccaacagc uuuaucaauc ugcugacccu caccgcccug     3960

ggcgcgcccg cugacuucaa cuuucuugga gaaaagauuc cuagaaagcg cuauacaucu     4020

accaaagagu gucugacagc cacgcucauc caccagucca uuaccggccu guacgaaaca     4080

cggaucgauc ucagcaagcu gggggaggaa uga                                  4113


<210>  4286
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4286
augaagaacg ccaauaaagu gaccggcaag uacuaucugg gguucgacau cggaacaaac       60

agcgucggcu gggcuguuac ggaugagaau uacaaccucu gcaaauuuaa gaaaaagucc      120

auguggggga uuagacuguu cgaagacgcc aagaccgcgg aggaacggcg cggacagaga      180

gcuucucggc gcagacuuca acggcgcaaa cagagaaucg aucugcucca ggagauuuuu      240

gccgaagaga ucaauaaggu ggacgagaca uucuuuauuc ggcugaacga aagccgccuu      300

caccuggaug acaaaaccgu guccgagaag cauccccucu ucaucggcuc ugaauauagc      360

gagaaagagu acuauagaga auacccuaca aucuuucauc ugcggaagga gcugauggaa      420

uccucugagc cgcacgauau ucgccucguc uaucuggcca uccaccauau ucuuaagaau      480

agagggcacu uccugaucga cggagaacuc agcaaagcua acuccuuuga ugccgcuuuc      540

uaccagauga aggaggugcu guguaaugag cuugaccugg aaauugaugg cgagaacacc      600

acagaaguug agcggacccu ccgcgacaga aauaucagca aaucugauaa ggugaaacug      660

cuggccgcgc uccuggccau gaaccaaaau gaaacggagu ccaaggagac caaaaagaag      720

auugaaaaca ucugcaaacu ucuggcuggc aauaaggggg acaucagcaa aauuuuugga      780

cagacacccg agggcgucga aaagaccucu uucuccuuug gggaggccga cuacgaugaa      840

agcauccggc cuaaccucga ggagucuauu cccgaccagu guuucgugau cgauuccauu      900

aaagcucugu augacuggaa cguucuugcc gauauccuga auggacagac auaccucagc      960

ugcgcgaagg ugaaccaaua ugaaacccac aagaauaacc ugaaagagcu gaagcuccug     1020

gucaauagau acuguucccg cgaagaguau aaacgguucu uuaagucugc cguggagaaa     1080

gcuaacuaca gcaauuauau cggcuccgcc aagcagggga acaagauuau cgacgugaaa     1140

agaugcgcug aagaggauuu cuacaaggaa cuuaaaauuc ugcucagcaa gaucgagccu     1200

gaagaggccg accgcgagcu guacgaacgg auuaaaagag aggccgaaga gggaucucuu     1260

cugccccucc agcgcaauaa ggauaacucc gucguuccua aucagaugca uaaggcugaa     1320

cuggcgcuga uccucgagaa cgccggcagc uaucugggcu uucuuaauga gaaagacgaa     1380

gaugggcugu cuccggcuga gaagaucauu gaacucuuca gcuuucggau ccccuacuau     1440

gugggaccuc uguccgacag acacaaagac cgcggcucua acgccuggau ugugcggaag     1500

gccgagggga gaauccugcc cuggaauuuc gaugaaaaag ucgaccgcga aaagagcaac     1560

gaggcuuuua uugaacggau gacgaacaaa uguacauacc uucucggaaa ggaugugguu     1620

gccaaaaauu cccugaucua uagccauuuc auggugcuga acgagcucaa uaaccugucu     1680

gucugcggca auaaaauuuc cgugcaaguu aagcaggaca ucuacagaga acuuuuucag     1740

cggcacacca aagugagcaa gaaaaugcug cuccaauauu gucgcgagaa gaugaugaac     1800

aaggaucugg aggaagagga ccugucuggg uucgauggag acuuuaaagc gaaacuuucc     1860

agcuaccucg auuucgaaaa gaaggucuuu ggcgcuaaaa uggaggaaga cagaauccgg     1920

aagauuacag aggauaucau uagauggaag accaucuacg gcgacgaugg gaaaaugcug     1980

caggccgugg ucgagaagga auauccugga gagcuggacu ccgaccagcu caaagccgug     2040

aagcaguucc gcuacucugg cugggggaau uucagcgaag aguuucugaa cggaauuguu     2100

ggcaccgaaa aacccacagg gcaaauguuc acgaucaucg aggcucuuug gcagaccaau     2160

ugcaaccuga ugcagcuccu guccaagaau uuuacauuca uggaggaaau ugagaagcac     2220

aaccgggaac uggccggcca gaucaccgau auuucuuaug acaaacuugu gaaggaucuc     2280

uacaucagcc cggcuaauaa aagagccguc uggcaaacag ugcgcauugc ggaggaaguu     2340

aagaaaauca ugggauguga gccugccaag aucuuugugg agauggcuag aggcggggaa     2400

aaagagaaga agcggaccau uucccgcaaa aagaaacugc uggaacucua ugccgcuugc     2460

aagaaagacg ccagagauug gaaccgggag aucgaggacc gcgaugaaag agaguucaau     2520

agcgacaagc uguaccuuua uuacacgcag augggacggu gcauguacuc uggcgaacgc     2580

auugaucugc augagcucau guccgcgaac agcaaguggg acaucgauca cauuuauccc     2640

cagucuaaag ucaaggacga uucccuggac aacagagugc ugagccuuaa aaagaucaau     2700

aacgaaaaag ggaauaagcc ucucucucgg gacauccagc aaagccagca gaaguggugg     2760

acagagcugc ugcagcucga guuuauuacc aaaaagaaau acgaucgccu gaugcauaga     2820

ggagaacuuu ccgaggagga gcugagcggc uucaucaacc ggcaacucgu ggaaaccaga     2880

cagucuacaa aggccgcugc cgagcugcug gggcgcaugu auaauaacuc caaaguuguc     2940

uacgugaagg cuccccuugu gagccaguuu cggaaggacg aucugaauau ucucaaaucc     3000

agacuggcca acgacuauca ccaugccaag gaugcuuacc ucaauaucgc ggucggcaac     3060

guguauaaug agaaauucac cgacaacccc cugcgcugga uugaaaagca cgccggagag     3120

aauuacucua ucaaccgggu uuuugauuac gacauugugg cuggcgaugg ggccccuguc     3180

ugggccgcuc cgcuuagaga cgaaaagaau aagcugcgca agaacgaggu gggagaaaaa     3240

uauggcggga caaucgaugu uauccggaag acggugaaaa gaaacgacgu ccucuacacc     3300

gagcacacau auuguggaaa gggcgagcug uucaaugcca ccauugcgaa aaaggacggg     3360

ggcgcugaaa ucccccugaa agccggacuu caaacaggca aguacggggg auauaagagc     3420

ccuaacaccu ccuacuuugc cgcuauugag uucgauggca aaaaggggga acgcgugaaa     3480

aauaucaugg agguucccgu guauauugcc aacauccugc uccaugacau cgaugcguac     3540

auucgguacu gcgagacggu caagggacug aaaaauguga agauccucag accuuguauu     3600

aagaaaaacg cucugaucuc uguggacggc uaucccaugc ggauucgcgg ggccaaugaa     3660

aagaacaucc uucugaaaaa uaaccuccag ccgaucuucg gcagacacga ggaaaccguc     3720

cggcgcauug agaaguacgu ugagaaaaau caggaauuug agguggauga aaaguucgac     3780

ggacugaacg aggccaaucu gacagaucuu uaugacgaua ucaccgaaaa gcugugcggc     3840

ccuuacgugg agagacccag caaccagggg gaauaucuca uggagaaucg ggagaaauuu     3900

cugaacauuu cccucaagga aaaagcuaag cugcuugccc aaaugaucaa acuggucaga     3960

ugugacacag agaccaaggc ugaucugcgc ucuauuggag ccagcaagaa ugcgggcgug     4020

aucuccguua acaaaaacac gcucggggga aagcggcuga uucuuaucaa ucagagcgug     4080

acaggccugu ucgaaaacag aaucgagcuc uga                                  4113


<210>  4287
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4287
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcu cugcuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acaaugcaag aggaaaagua cuaucaugag aaauucccca ccaucuacca ucugcggaag      420

gaacuggcug acaagaaaga gaaggccgau cuccgccuga uuuaccuugc ucuggcccac      480

aucauuaaau uuagaggaca cuuccucauc gaggacgauc gguuugacgu ccgcaacaca      540

gacauucaga agcaguauca ggcguuccug gaaaucuuug auaccacguu cgagaauaac      600

gaccuucugu cccaaaaugu ggauguugaa gccauccuca ccgacaaaau uucuaagagc      660

gcuaaaaagg auagaauccu ggcccgguac ccuaaccaga aguccacagg cauuuuugcu      720

gaguuccuga aacucaucgu gggcaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacac cccuucaguu cgcgaaagac agcuaugaug aggaccugga gaaucuccug      840

gggcaaauug gagaugaauu ugccgaccuu uucucugcug ccaagaagcu guacgacucc      900

guccuccuga gcggcauccu gaccgugacc gaucucucua cgaaagcucc ucuguccgcc      960

agcaugaucc agagauauga cgagcaccgc gaagaucuua agcagcugaa acaguuuguu     1020

aaggccucuc uccccgagaa auaccaagaa auuuucgcug acuccagcaa ggauggguau     1080

gcgggauaca ucgagggcaa gacaaaccag gaggccuuuu acaaauaucu guccaaacuu     1140

cugaccaaac aggaaggguc ugaguaccuc cuggaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaaa gaacauuuga uaacggaagc aucccucacc aggugcaucu caccgaacug     1260

cgcgcuauua uccggagaca guccgaguau uacccguucc uuaaggagaa ccuggaccgc     1320

aucgaaaaga uucucaccuu ucggaucccc uauuacgucg gcccucuggc cagagaaaag     1380

agcgauuucg ccuggaugac acgcaaaacg gacgauucua uucggcccug gaauuuugaa     1440

gaccuggugg auaaggagaa auccgcugag gccuucaucc acagaaugac caacaaugac     1500

cuuuaucucc cugaagagaa ggugcugccc aaacauagcc ugauuuacga aaaguucaca     1560

gucuacaacg agcucaccaa gguucgcuuu cuggcggaag gcuucaaaga cuuucaguuc     1620

cuuaaucgga agcaaaaaga gacaaucuuu aacucucugu ucaaggagaa aagaaaggug     1680

accgaaaagg auauuaucag cuuucucaau aaaguggacg gguaugaggg aaucgcuauu     1740

aagggcaucg aaaaacaguu caacgccucc cugucuacgu accacgaucu gaagaaaauu     1800

cuugggaagg acuuucucga uaauacagac aacgagcuga uccuggaaga uauuguccag     1860

acccucaccc uguucgagga ccgggagaug auccgcaaga gacuugauau cuauaaagac     1920

uuuuucacag aaagccagcu gaagaaacuc uaccggagac acuauaccgg auggggccgc     1980

cuguccgcca agcugauuaa ugggauccgg aacaaagaga aucaaaagac aauucuugau     2040

uaccucaucg acgauggaag cgcuaacaga aauuuuaugc agcugauuaa ggacgccggc     2100

cugucuuuca aaccgaucau cgacaaggcu cgcacgggcu cccauagcga uaaccucaaa     2160

gaagugguug gggagcuggc cggaucuccu gcgauuaaga aaggcauccu ucagucccug     2220

aagauugugg acgaacucgu caaagugaug ggguaugagc ccgagcagau cguuguggaa     2280

auggccagag agaaccaaac cacagcuaag ggacugagcc ggucccgcca gagacugacc     2340

acacuucggg aaucucucgc caaucuuaag agcaacauuc uggaggagaa aaagccuaaa     2400

uacgucaagg aucaggugga aaaucaccau cucuccgacg aucgccuguu ccuuuacuau     2460

cugcagaacg gcaaagacau guacaccgau gacgagcucg auaucgacaa ucugucucaa     2520

uaugauaucg accacauuau cccccaggcu uuuauuaagg acgauagcau cgacaacaga     2580

guccuggugu ccagcgccaa gaaucggggg aaaucugaug acguucccuc cguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa cuucuggacg cgaagcucau gagccagcgc     2700

aaguacgaua accugacgaa agccgagaga ggcggacuca ccucugacga uaaggcucgg     2760

uuuauccaac gccagcuggu ugaaacaaga cagauuacca aacauguggc ccggauccuu     2820

gacgagagau ucaauaacga gcuggauucc aagggccgcc ggaucagaaa agucaagauu     2880

gugacacuca agagcaaucu ggugucuaac uuucgcaaag aauuuggguu uuauaagauc     2940

cgggagguua auaacuacca ccacgcucau gacgccuauc ugaaugccgu cguggcuaaa     3000

gcgauucuua ccaaguaccc ucagcuggaa ccggaguucg uguacggaga uuaucccaaa     3060

uacaacagcu auaagacgag aaaguccgcc acagaaaaac ucuuuuucua cagcaacauc     3120

augaauuucu ucaagaccaa agucacacug gcugacggca ccgugguugu gaaggacgau     3180

auugagguca acaaugacac cggggagauc gugugggaua aaaagaaaca cuucgccaca     3240

guucgcaagg ugcucucuua uccucaaguc aacaucguga agaaaacgga aauucagacc     3300

ggaggcuuuu ccaaggagag cauccuggcc cauggcaauu ccgacaaacu uauuccccgg     3360

aagacaaaag auaucuaccu ggacccuaag aaguaugggg gauucgauuc ucccauuguu     3420

gcuuacagcg ugcucgucgu ggccgacauc aaaaagggca aagcgcagaa gcugaaaacc     3480

gugacagaac ugcuugggau caccauuaug gagagauccc gcuuugaaaa gaacccgucu     3540

gcuuuccugg agagcaaggg auaccucaau auccgggaug acaaacugau gauucucccu     3600

aaguauuccc uguuugagcu ugaaaacggc agacggcgcc ugcuggccuc ugccggggag     3660

cuccagaaag gaaaugaacu ggcccuuccc acgcaauuca ugaaguuucu guaccucgcu     3720

agcagauaua acgagcugaa aggcaagccu gaggaaaucg agcagaagca ggaguucguc     3780

guucagcacg uguccuacuu ugaugacauu cuccaaauca uuaaugauuu cagcaaccgg     3840

gugauccugg cggacgcuaa ucuugagaaa aucaacaagc uguaucagga caauaaagaa     3900

aacauucccg ucgaugagcu ggccaacaau aucauuaacc ucuuuacauu caccucucug     3960

ggggccccug cugccuuuaa guucuucgac aaaaucgugg aucgcaagag auacaccucc     4020

acaaaagagg uucuuaauag cacccugauu caccagucua ucacaggccu cuaugaaacg     4080

cggaucgacc ugggaaagcu cggcgaggau uga                                  4113


<210>  4288
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4288
augaccaaga ucaaagacga uuacauugug ggccuggaca ucgggacaga uagcugcgga       60

ugggucgcca ugaacuccaa uaacgacauu cucaagcugc agggcaaaac ggcuaucggg      120

ucuagacuuu ucgagggagg caagagcgcc gcggaacggc gccuguuuag aaccacacac      180

cggcgcauua aaagacggcg cuggagacuc aagcugcuug aggaguucuu ugaucccuau      240

auggcugagg uugacccuua cuucuuugcc cggcugaagg aauccgggcu cucuccgcug      300

gauaaacgca agaccgugag cuccaucgug uuccccacau cugccgagga caaaaaguuu      360

uaugaugacu acccuaccau cuaucaucug agauacaaac ucaugacaga ggaugaaaag      420

uucgaccugc gggaggucua ucuugcuauu caccauauca uuaaguaccg cggaaauuuu      480

cuguacaaca ccagcgugaa agauuucaag gccuccaaaa ucgacguuaa gagcucuauu      540

gaaaaacuca augagcugua ugagaaccuu ggccuggacc ucaaugugga auuuaacauc      600

uccaauacgg cugagauuga aaagguccug aaagauaagc aaaucuucaa gagagacaaa      660

gugaagaaaa ucgccgagcu guuugcgauu aagaccgaua acaaagaaca gagcaagcgg      720

aucaaggaca uuucuaaaca gguugccaau gcugugcucg gcuacaagac acgcuucgau      780

accaucgccc ugaaagagau uuccaaggac gagcuuagcg auuggaacuu caaacugucu      840

gacaucgaug cugacuccaa guuugaagcc cucaugggga aucuggauga gaacgaacag      900

gcgauccuuc ugacaauuaa ggaacucuuc aaugaaguca cccugaacgg aaucguggag      960

gacggcaaca cgcugagcga guccaugauu aauaaauaua acgaccacag agaugaccuc     1020

aagcugcuua aagaagugau cgagaaucau auugaucgga agaaagccaa ggaacuggcu     1080

cucgccuacg accuguaugu caacaauaga cacgggcgcc uucugcaagc uaagaaaaag     1140

cucggaaaaa ucaagccccg gucuaaagag gauuuuuaca agguugugaa caaaaaucug     1200

gacgauagca gagccuccaa ggaaaucaag aaaaagauug agcuggacag cuucaugccu     1260

aaacagcgca caaacgccaa uggcgugauc cccuaucagc uccagcaacu ggagcuugau     1320

aagauuaucg aaaaccaguc uaaauacuac ccuuuucuga aggagauuaa uccggucucc     1380

agccaccuca aggaagcucc cuauaaacug gacgagcuga uccgguucag agugccuuac     1440

uauguugggc cccuuauuuc uccuaacgag agcaccaagg auauccagac caaaaagaau     1500

cagaacuuug cguggaugau ccgcaaagaa gagggacgga uuacacccug gaauuucgac     1560

caaaaggugg auagaaucga auccgccaac aaguuuauua aacgcaugac cacaaaggac     1620

acguaccucu ucggcgagga cguccugccg gcuaacucuc ugcucuauca gaaauuuacc     1680

gugcugaaug aacuuaacaa uauccggauu aacggcaaga gaaucagcgu ugaucugaaa     1740

caggagaucu acgagaaucu cuucaagaag cauacaaccg ugacagucaa aaagcuggaa     1800

aacuaucuga aagagaauca caaccuugug aaggucgaaa uuaaagggcu cgccgacgag     1860

aagaaauuua auuccggacu gaccacguac aaccgcuuca agaaucugaa caucuucgau     1920

aaucaaauug acgaucucaa guaccggaac gacuuugaga aaaucauuga guggagcaca     1980

aucuucgagg auaagucuau cuauaaagaa aagcugagau ccauugacug gcuuaaugag     2040

aaacagauca acgcccugag caacauucgg cuccagggcu gggggcgccu gucuaagaag     2100

cugcuugcuc agcuccauga ucacaaugga caaaccauca uugaacagcu gugggacucc     2160

cagaacaauu uuaugcagau cgugacccaa gccgauuuca aagacgcgau cgcuaaggcc     2220

aaccagaauc ugcucguugc cacaagcgug gaggauauuc ugaacaaugc uuacaccucc     2280

ccugccaaca aaaaggcuau cagacagguc auuaaagugg uugacgacau cgugaaggcc     2340

gcgucuggca agguccccaa acagauugcc aucgaauuua cacgggaugc ugacgagaau     2400

ccuaagagaa gccaaacgcg cggguccaaa cuucagaagg uguauaaaga ucugucuacc     2460

gagcucgcca gcaagacaau ugcugaagaa cugaacgaag ccaucaagga caaaaagcug     2520

gugcaggaua aauacuaucu uuacuucaug cagcucggac gggacgcgua uaccggcgag     2580

cccaucaaua uugaugagau ccaaaaguac gacauugauc acauccugcc ccaguccuuu     2640

auuaaagacg augcccugga caacagaguu cucgucagcc gcgcugugaa uaacggcaag     2700

ucugacaaug ugccugucaa gcuguucggg aacgaaaugg ccgcuaaccu uggaaugaca     2760

aucagaaaaa ugugggagga auggaagaau aucggccuga uuuccaaaac caaguacaac     2820

aaucuccuga cggauccgga ccauaucaac aaauauaaga gcgccggguu uauucggcgc     2880

cagcuggugg agaccucuca aaucauuaaa cuuguuucca caauccugca gagcagauac     2940

cccaauaccg aaaucauuac agugaaggcc aaguauaacc acuaccuccg ggagaaauuc     3000

gaucuguaua agucucgcga ggucaaugac uaccaucacg cuaucgaugc guaucucagc     3060

gccauuugug gaaaccugcu uuaccagaau uacccuaacc ugagacccuu uuucguguau     3120

ggccaguaca aaaaguuuuc cagcgacccu gauaaagaaa aggagaucuu caauaagacc     3180

cggaaauucu cuuuuauuuc ccaaauccuc aagaacaaaa gcgaaaauuc caaggagauu     3240

gcugaaaaac ugaagcgcgc cuaucaguuc aaguacaugc ugguuucuag agagacggag     3300

acaaaagacc aggaaauguu uaagaugacc guguuccccc gggagacacc ggggaaccuu     3360

aucccuaaaa agaaaggcau ggcccccgaa aucuauggag gcuacaccaa gaauagcgau     3420

gcuuauaugg ucauugugaa gaucgacaaa aagaaagaga ccgaauaccg cauucugggg     3480

aucccuacaa gagagcucgu uaaccugaag aaagccgaga aggaagauca uuacgagucc     3540

uaucucaaag aaauucuggc gccccggauc cuuuacaaua agaacggaaa gagagacaaa     3600

aagaucacgu cuuuugagau ugugaaaagc aagaucccuu auaaacaggu cauucaagau     3660

ggcgacaaga aguucaugcu gggguccucu accuacgugu auaacgcuaa acagcucaca     3720

cugagcaccg aguccaugaa ggccaucaca aauaacuuug acaaagauag cgacgaaaau     3780

gaugcccuga uuaaggcuua cgacgagauc cuugauaaag uggacaagua ucugccccuc     3840

uucgauauca acgccuuucg caagaaacug cacucuggac gggaaaaguu cgucgagcuc     3900

ucccuggaag acaaaaagga uauuauccuu aaaguucuga uuggccugca cgacaauccg     3960

gcgaccagcg aucucaagac gcugggguuu ucuuccacag agcuuggcaa gaugcagaaa     4020

aacagcggaa ucgugcuguc uggcaaugcu aagcucauuu accagucccc uaccgggcug     4080

uucaaaaaga gcgugaaaau cuccgaccuc uga                                  4113


<210>  4289
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4289
augaccaaga ucaaagacga uuacauugug ggccuggaca ucgggacaga uagcugcgga       60

ugggucgcca ugaacuccga caaugauauu cucaagcugc agggcaaaac ggcuaucggg      120

ucucaccuuu ucgaggccgg aaagagcgcg gcugacagac ggcuguuucg caccacacau      180

agacggauua aacgcagacg guggcgccuc aagcugcuug aagaguucuu ugaucccuau      240

auggccaagg uugacccuua cuucuuugcc agacugaaag aauccggccu cucuccgcug      300

gauaagcgga aaaacguguu cagcaucgug uuucccaccg cugccgagga caagaaauuc      360

uaugaugacu acccuacaau cuaucaccuc cgcuacaagc ucaugaccga agaugagaag      420

uuugaccuga gagaggucua ucuugcuauu caucacauca uuaaauaccg ggggaauuuc      480

cuguacaaca cauccgugaa ggacuuuaaa gccucuaaga ucgauguuaa aagcuccauu      540

gaaaagcuca augagcugua ugaaaaccuu ggacuggacc ucaaugugga guucaacauc      600

agcaauaccg cggagauuga aaaaguccug aaggauaagc aaaucuuuaa acgcgacaag      660

gugaaaaaga ucgccgagcu guucgcuauu aaaacggaua acaaggaaca gucuaagaga      720

aucaaagaca uuuccaagca gguugccaau gcugugcucg gcuacaaaac ccgguucgau      780

acaaucgccc ugaaggaggu cagcaaagac gaacuuucug auuggaacuu uaagcugucc      840

gacauugaug cggacagcaa guucgaggcc cucaugggca aucuggacga gaacgaacag      900

gcuauccuuc ugaccaucaa agagcucuuu aaugccguga cacugaacgg gauuguggaa      960

gauggaaaca cccugucuga guccaugauc aauaaguaua acgaccaucg cgaugaccuc     1020

aaacugcuua aggaagucau ugagaaucac aucgauagaa agaaggcugu uaagcuggcc     1080

cucgccuacg accuguaugu gaacaaucgg cacggccaac uuauucaggc uaaaaagaaa     1140

cuggggaaga ucaaacccgg aagcaaggag gauuucuaca aaguggucaa caagaaucuc     1200

gacgauuccc aggcgucuaa ggaaaucaaa aagaaaauug agcuggacag cuuuaugccu     1260

aagcagagaa cgaacgccaa uggcgugauc cccuaucaac ugcaucagcu cgaacuggau     1320

aaaauuaucg agaaccaguc caaguacuac ccuuuccuua aggaaauuaa uccgguuagc     1380

ucucaccuga aagaggcucc cuauaagcuc gacgagcuga uccgcuuucg ggugccuuac     1440

uaugucgggc cccugauuuc cccuaacgaa agcacaaaag auauccagac caagaaaaau     1500

caaaacuucg ccuggaugau cagaaaggag gaaggacgca uuacacccug gaauuuugac     1560

cagaaggugg accggaucga gucugccaac aaauucauua agagaaugac caccaaagau     1620

acauaccuuu uuggcgagaa cguucucccg gcuaauagcc ugcuguauca gaaguucacg     1680

gugcucaacg aacugaauaa cauccgcauu aauggcaaac ggaucuccgu cgaccuuaag     1740

caagagaucu acgaaaaccu guuuaagaaa cauaccacag ugaccgucaa gaaacucgag     1800

aauuaucuga agcagaacca caaucuggug aaaguugaaa uuaaggggcu ugccgaugag     1860

aaaaaguuca acucuggacu cacaaccuac aauagauuca agaaccugaa uaucuuugac     1920

aaccagauug augaccugaa auaccgcaag gauuucgaga aaaucauuga auggagcacg     1980

aucuuugagg acaaguccau cuauaaagaa aagcuccgga gcauugauug gcugaaugag     2040

aagcagauca acgcgcuuuc uaacauuaga cugcaaggcu gggggcgccu cuccaaaaag     2100

cugcuggcuc agcuucacga ccauaaugga cagacaauca uugaacagcu cugggauagc     2160

caaaacaauu ucaugcagau cgugacccag gccgacuuua aagaugccau cgcuaaggcc     2220

aaccagaauc ugcuggucgc uaccucugug gaggacauuc ucaacgacgc cuacacaucc     2280

ccugcgaaua aaaaggccau ccggcaaguu auuaaggugg ucgaugacau cgugaaagcu     2340

gccagcggca aggugcccaa acagauugcu aucgaguuca ccagagaugc cgacgauaac     2400

ccuaagcggu cccagacacg cgggucuaaa cugcagaagg uuuauaagga ccuuagcacg     2460

gaacuggcgu ccaaagccau ugcugaggaa cucaaugagg ccaucaagga uaaaaagcug     2520

guccaagaca aauacuaucu guacuuuaug cagcuuggaa gagaugcuua uaccggcgaa     2580

cccaucaaca uugacgagau ccagaaguac gauauugacc acauccuccc ccaaucuuuc     2640

auuaaggacg augcccugga caaucgggug cuggugagca aagccgucaa caaugauaag     2700

uccgacaacg ugccuguuaa acucuuuggc aaugagaugg cugggaaccu gggaaucaca     2760

aucagaaaga ugugggaaga guggaaaaac auuggccuua ucagcaagac caaauacaau     2820

aaccugcuca cagauccgga ccauauuaau aaguaugaau cugcgggguu cauccgccgg     2880

cagcuggugg agaccuccca gauuaucaag cuggucagca cgauccuuca gucuagauac     2940

cccaacaccg agauuaucac agugaaagcc aaguauaauc acuaccugcg cgaaaaauuu     3000

gaucucuaua aguccagaga gguuaacgac uaccaucacg cuauugaugc cuaucugagc     3060

gccaucugug gaaaucuccu guaccaaaac uacccuaauc uucggcccuu cuuuguguau     3120

ggccaguaca aaaaguucuc uagcgacccu gauaaggaaa aagagauuuu caacaagacc     3180

cgcaaauuuu ccuucaucag ccagauucug aagaauaaau cugaaaacuc caaggagauc     3240

gcugagaagc ucaaaagagc cuaucaguuu aaguacaugc uggucagccg ggaaacagag     3300

accaaagacc aagaaauguu caagaugacg guguuucccc gcgagacacc ggggaaucug     3360

aucccuaaaa agaagggcau ggcgaccgaa auuuauggag gcuacaccaa aaacuccgau     3420

gcuuauaugg uuaucgugaa gauugacaaa aagaaagaga cagaguacag aauccuuggg     3480

auucccaccc gggaacuggu caaucucaag aaagccgaga aggaagacca cuacgagucu     3540

uaucugaagg aaauccucgc cccucgcauc cuguacaaca aaaauggaaa gagagauaaa     3600

aagauuacga gcuucgagau cgugaaaucc aagauucccu auaaacaggu gauccaggac     3660

ggcgauaaaa aguuuaugcu ugggucuagc acauacgucu auaacgcuaa acagcugacc     3720

cucuccacag agucuaugaa ggccauuacc aacaauuucg acaaagauag cgacgaaaac     3780

gaugcgcuga ucaaggcuua cgacgagauc cuggauaagg uugacaaaua ucuuccucug     3840

uuugauauua augccuuccg gaagaaacuc cauuccggac gcgaaaaguu uguggagcug     3900

agccucgagg acaaaaagga uaucauucug aaggugcuua ucggccugca cgacaacccc     3960

gccacaucug accugaaaac ccucggguuc uccagcacgg aacugggcaa gaugcagaaa     4020

aauucuggaa uuguccuuuc cggcaacgcu aagcugaucu accaaagccc gacagggcug     4080

uucaaaaagu cugugaaaau uuccgaucug uga                                  4113


<210>  4290
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4290
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcu cugcuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acaaugcaag aggaaaagua cuaucaugag aaauucccca ccaucuacca ucugcggaag      420

gaacuggcug acaagaaaga gaaggccgau cuccgccuga uuuaccuugc ucuggcccac      480

aucauuaaau uuagaggaca cuuccucauc gaggacgauc gguuugacgu ccgcaacaca      540

gacauucaga agcaguauca ggcguuccug gaaaucuuug auaccacguu cgagaauaac      600

gaccuucugu cccaaaaugu ggauguugaa gccauccuca ccgacaaaau uucuaagagc      660

gcuaaaaagg auagaauccu ggcccgguac ccuaaccaga aguccacagg cauuuuugcu      720

gaguuccuga aacucaucgu gggcaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacac cccuucaguu cgcgaaagac agcuaugaug aggaccugga gaaucuccug      840

gggcaaauug gagaugaauu ugccgaccuu uucucugcug ccaagaagcu guacgacucc      900

guccuccuga gcggcauccu gaccgugacc gaucucucua cgaaagcucc ucuguccgcc      960

agcaugaucc agagauauga cgagcaccgc gaagaucuua agcagcugaa acaguuuguu     1020

aaggccucuc uccccgagaa auaccaagaa auuuucgcug acuccagcaa ggauggguau     1080

gcgggauaca ucgagggcaa gacaaaccag gaggccuuuu acaaauaucu guccaaacuu     1140

cugaccaaac aggaaggguc ugaguaccuc cuggaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaaa gaacauuuga uaacggaagc aucccucacc aggugcaucu caccgaacug     1260

cgcgcuauua uccggagaca guccgaguau uacccguucc uuaaggagaa ccuggaccgc     1320

aucgaaaaga uucucaccuu ucggaucccc uauuacgucg gcccucuggc cagagaaaag     1380

agcgauuucg ccuggaugac acgcaaaacg gacgauucua uucggcccug gaauuuugaa     1440

gaccuggugg auaaggagaa auccgcugag gccuucaucc acagaaugac caacaaugac     1500

cuuuaucucc cugaagagaa ggugcugccc aaacauagcc ugauuuacga aaaguucaca     1560

gucuacaacg agcucaccaa gguucgcuuu cuggcggaag gcuucaaaga cuuucaguuc     1620

cuuaaucgga agcaaaaaga gacaaucuuu aacucucugu ucaaggagaa aagaaaggug     1680

accgaaaagg auauuaucag cuuucucaau aaaguggacg gguaugaggg aaucgcuauu     1740

aagggcaucg aaaaacaguu caacgccucc cugucuacgu accacgaucu gaagaaaauu     1800

cuugggaagg acuuucucga uaauacagac aacgagcuga uccuggaaga uauuguccag     1860

acccucaccc uguucgagga ccgggagaug auccgcaaga gacuugauau cuauaaagac     1920

uuuuucacag aaagccagcu gaagaaacuc uaccggagac acuauaccgg auggggccgc     1980

cuguccgcca agcugauuaa ugggauccgg aacaaagaga aucaaaagac aauucuugau     2040

uaccucaucg acgauggaag cgcuaacaga aauuuuaugc agcugauuaa ggacgccggc     2100

cugucuuuca aaccgaucau cgacaaggcu cgcacgggcu cccauagcga uaaccucaaa     2160

gaagugguug gggagcuggc cggaucuccu gcgauuaaga aaggcauccu ucagucccug     2220

aagauugugg acgaacucgu caaagugaug ggguaugagc ccgagcagau cguuguggaa     2280

auggccagag agaaccaaac cacagcuaag ggacugagcc ggucccgcca gagacugacc     2340

acacuucggg aaucucucgc caaucuuaag agcaacauuc uggaggagaa aaagccuaaa     2400

uacgucaagg aucaggugga aaaucaccau cucuccgacg aucgccuguu ccuuuacuau     2460

cugcagaacg gcaaagacau guacaccgau gacgagcucg auaucgacaa ucugucucaa     2520

uaugauaucg accacauuau cccccaggcu uuuauuaagg acgauagcau cgacaacaga     2580

guccuggugu ccagcgccaa gaaucggggg aaaucugaug acguucccuc cguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa cuucuggacg cgaagcucau gagccagcgc     2700

aaguacgaua accugacgaa agccgagaga ggcggacuca ccucugacga uaaggcucgg     2760

uuuauccaac gccagcuggu ugaaacaaga cagauuacca aacauguggc ccggauccuu     2820

gacgagagau ucaauaacga gcuggauucc aagggccgcc ggaucagaaa agucaagauu     2880

gugacacuca agagcaaucu ggugucuaac uuucgcaaag aauuuggguu uuauaagauc     2940

cgggagguua auaacuacca ccacgcucau gacgccuauc ugaaugccgu cguggcuaaa     3000

gcgauucuua ccaaguaccc ucagcuggaa ccggaguucg uguacggaga uuaucccaaa     3060

uacaacagcu auaagacgag aaaguccgcc acagaaaaac ucuuuuucua cagcaacauc     3120

augaauuucu ucaagaccaa agucacacug gcugacggca ccgugguugu gaaggacgau     3180

auugagguca acaaugacac cggggagauc gugugggaua aaaagaaaca cuucgccaca     3240

guucgcaagg ugcucucuua uccucaaguc aacaucguga agaaaacgga aauucagacc     3300

ggaggcuuuu ccaaggagag cauccuggcc cauggcaauu ccgacaaacu uauuccccgg     3360

aagacaaaag auaucuaccu ggacccuaag aaguaugggg gauucgauuc ucccaaaguu     3420

gcuuacagcg ugcucgucgu ggccgacauu aagaaaggca aggcgcagaa acugaagacc     3480

gugacagaac ugcuugggau caccaucaug gagagauccc gcuuugaaaa gaacccgucu     3540

gcuuuccugg agagcaaagg auaccucaau auucgggaug acaagcugau gauccucccu     3600

aaauauuccc uguuugagcu ugaaaacggc agacggcgcc ugcuggccuc ugccggggag     3660

cuccagaagg gaaaugaacu ggcccuuccc acgcaauuca ugaaauuucu guaccucgcu     3720

agcagauaua acgagcugaa gggcaagccu gaggaaauug agcagaaaca ggaguucguc     3780

guucagcacg uguccuacuu ugaugacauc cuccaaauua ucaaugauuu cagcaaccgg     3840

gugauucugg cggacgcuaa ucuugagaag aucaacaaac uguaucagga caauaaggaa     3900

aacauccccg ucgaugagcu ggccaacaau auuaucaacc ucuuuacauu caccucucug     3960

ggggccccug cugccuuuaa auucuucgac aagauugugg aucgcaaaag auacaccucc     4020

acaaaggagg uucuuaauag cacccugauc cacaagucua uuacaggccu cuaugaaacg     4080

cggaucgacc ugggaaaacu cggcgaggau uga                                  4113


<210>  4291
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4291
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcc cuucuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acacugcaag aggaaaagga cuaccaugag aaauucucca ccaucuauca ucugcggaag      420

gaacucgcug auaagaaaga gaaggccgac cugcgccuua uuuacaucgc ucuggcccac      480

auuaucaaau uuagaggaca cuuccucauu gaggaugacu cuuuugacgu ccggaacaca      540

gauaucagca agcaguacca ggacuuccug gaaaucuuua auaccacguu cgagaacaau      600

gaucuucugu cccagaacgu ggacguugaa gcgauucuca ccgauaaaau cagcaagucu      660

gccaaaaagg accgcauucu ggcucaauau cccaaucaga aguccacagg caucuuugcc      720

gaguuccuga aacucauugu gggcaaccag gcugauuuca agaaauacuu uaaucuggaa      780

gacaagaccc cucuucaguu cgccaaagau agcuaugacg aggaucugga gaaccuccug      840

gggcaaaucg gagacgaauu ugcggaccuu uucucugccg cuaagaagcu guacgauucc      900

guccuccuga gcggcauccu gacagugauu gaccucucua ccaaagcucc ccuguccgcu      960

agcaugaucc agagauauga ugagcaucgg gaagaccuua agcagcugaa acaguuuguu     1020

aaggccuccc ucccugagaa auaccaagaa auuuucgccg auucuagcaa ggacggguau     1080

gcuggauaca ucgagggcaa gacgaaucag gaggcguuuu acaaauaucu guccaaacuu     1140

cugacaaagc aggaagauag cgagaacuuc cucgaaaaga uuaaaaacga ggacuuucug     1200

agaaagcaac gcaccuucga uaaugggucu aucccccacc aggugcaccu gacagaacuc     1260

aaggccauca uucggagaca guccgaguac uauccuuuuc ugaaagagaa ccaggaccgc     1320

aucgaaaaga uucuuaccuu ccggaucccg uacuauauug gaccccuggc uagagagaaa     1380

agcgauuuug ccuggaugac ccgcaagaca gacgauucua uccggccuug gaauuucgaa     1440

gaccucgucg acaaagaaaa gagcgccgag gcuuucauuc auagaaugac gaacaaugau     1500

uuuuaccugc ccgaagagaa agugcugccu aagcacuccc uuaucuauga aaaguucacc     1560

guguacaacg agcucacaaa aguccgcuac aagaaugaac aaggcgagac cuauuucuuc     1620

gacucuaaca ucaaacagga gauuuuugau ggcguuuuca aggaacaucg gaaagugagc     1680

aagaagaaac ugcuggacuu ucucgccaag gaguacgaag aguucagaau cguggauguc     1740

auugggcugg acaaagaaaa uaaggcguuu aacgcuuccc uuggaacaua ucacgaucug     1800

gagaaaaucc ucgacaagga uuuccuggac aaucccgaua acgagagcau ucuggaagac     1860

aucgugcaga cccuuacgcu cuuugaggau cgcgaaauga ucaagaaacg gcuggagaau     1920

uacaaggacc uguucacaga aucucagcuc aaaaagcugu auagacggca cuacaccggc     1980

ugggggcgcc uuuccgccaa acugauuaac ggaaucagag acaaggagag ccaaaagacc     2040

auucucgauu aucugaucga cgauggccgg ucuaauagaa acuuuaugca gcugauuaac     2100

gacgaugggc uuuccuucaa aagcaucauc uccaaggccc aggcuggauc ucauagcgac     2160

aaucucaaag agguuguggg cgaacuggcc ggcuccccgg cuauuaagaa agggauccug     2220

cagucucuca agauugucga ugagcuggug aaaguuaugg gauacgaacc ugagcaaauc     2280

guggucgaga uggcccgcga aaaccagaca accaaucagg gccggagaaa cagccgccag     2340

agauacaaac uucuggacga uggggugaag aaucucgcgu ccgaccugaa cggaaauauu     2400

cugaaggagu aucccacaga uaaccaagcc cuucagaaug aacgccucuu ccuguacuau     2460

cugcagaacg gccgggacau guacacgggg gaggcucucg acaucgauaa ucugagccaa     2520

uaugacaucg aucacauuau cccucaggcc uuuauuaagg acgauucuau cgacaacaga     2580

guccuugugu ccagcgcuaa aaaucggggc aagucugaug acguucccuc ccuggaaauu     2640

gugaaagauu gcaaggucuu cuggaagaaa cuccuggacg ccaagcugau gagccagcgc     2700

aaauacgaua accuuaccaa ggcggagaga ggaggccuga caucugacga uaaagcccgg     2760

uuuauccagc gccaacucgu ggagaccaga cagauuacaa agcauguugc ucggauccug     2820

gacgaacgcu ucaauaacga gcucgacagc aaggggagac ggaucagaaa agugaagauu     2880

gucacccuga aauccaaccu ugugagcaau uuucgcaagg aauuuggauu uuauaaaauc     2940

cgggagguga acaauuacca ccaugcccac gaugcuuacc ugaacgccgu ugucgccaag     3000

gcuauucuca cgaaguaucc ccagcuggaa ccugaguucg uguacggcga cuauccgaaa     3060

uacaauucuu auaagaccag aaaauccgcg acagagaagc uguuuuucua cagcaacauc     3120

augaacuuuu ucaaaaccaa ggugacacuu gccgauggga ccgucguggu uaaggacgau     3180

auugaaguga auaaugacac gggagagauc gucugggaua aaaagaaaca cuucgcuaca     3240

gugcgcaagg uucuguccua uccccagaac aauaucguga aaaagaccga aauucaaaca     3300

ggcggguuuu cuaaagagag cauccucgcc cauggcaacu ccgacaagcu gauuccucgg     3360

aagaccaaag auaucuaccu cgaccccaag aaauacggag gcuucgauuc uccuauuguc     3420

gccuauagcg ugcugguugu ggcugacauc aagaaaggga aggcccagaa acuuaagacc     3480

gucacagaac ugcucggaau cacgauuaug gagagauccc gcuuugagaa gaaucccucu     3540

gcguuccugg aaagcaaagg cuaccugaac auccgggcug auaaacuuau uauccugccg     3600

aaguauuccc ucuuugagcu ggaaaauggg agacgccggc uccuggccag cgccggagag     3660

cuucagaagg gcaacgagcu ggcucugccu acccaguuca ugaaguuucu cuaccuggcc     3720

ucuagauaua acgaauccaa agggaagccc gaggaaauug agaaaaagca agaguucgug     3780

aaucagcacg ugagcuacuu ugacgacauc cuucagcuga uuaacgauuu cucuaaacgg     3840

gucauccucg cggacgcuaa ucuggagaag aucaacaagc ucuaucaaga uaauaaagag     3900

aacauuuccg uugacgaacu ggccaauaac aucauuaauc uuuuuacauu caccagccug     3960

ggcgccccug cugccuuuaa guucuucgau aaaaucgugg accgcaagag auacacaucu     4020

accaaagagg ugcugaacuc cacgcucauu caucagagca ucacaggacu guacgaaacc     4080

cggaucgauc uuggcaagcu gggggaggac uga                                  4113


<210>  4292
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4292
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcc cuucuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acacugcaag aggaaaagga cuaccaugag aaauucucca ccaucuauca ucugcggaag      420

gaacucgcug auaagaaaga gaaggccgac cugcgccuua uuuacaucgc ucuggcccac      480

auuaucaaau uuagaggaca cuuccucauu gaggaugacu cuuuugacgu ccggaacaca      540

gauaucagca agcaguacca ggacuuccug gaaaucuuua auaccacguu cgagaacaau      600

gaucuucugu cccagaacgu ggacguugaa gcgauucuca ccgauaaaau cagcaagucu      660

gccaaaaagg accgcauucu ggcucaauau cccaaucaga aguccacagg caucuuugcc      720

gaguuccuga aacucauugu gggcaaccag gcugauuuca agaaauacuu uaaucuggaa      780

gacaagaccc cucuucaguu cgccaaagau agcuaugacg aggaucugga gaaccuccug      840

gggcaaaucg gagacgaauu ugcggaccuu uucucugccg cuaagaagcu guacgauucc      900

guccuccuga gcggcauccu gacagugauu gaccucucua ccaaagcucc ccuguccgcu      960

agcaugaucc agagauauga ugagcaucgg gaagaccuua agcagcugaa acaguuuguu     1020

aaggccuccc ucccugagaa auaccaagaa auuuucgccg auucuagcaa ggacggguau     1080

gcuggauaca ucgagggcaa gacgaaucag gaggcguuuu acaaauaucu guccaaacuu     1140

cugacaaagc aggaagauag cgagaacuuc cucgaaaaga uuaaaaacga ggacuuucug     1200

agaaagcaac gcaccuucga uaaugggucu aucccccacc aggugcaccu gacagaacuc     1260

aaggccauca uucggagaca guccgaguac uauccuuuuc ugaaagagaa ccaggaccgc     1320

aucgaaaaga uucuuaccuu ccggaucccg uacuauauug gaccccuggc uagagagaaa     1380

agcgauuuug ccuggaugac ccgcaagaca gacgauucua uccggccuug gaauuucgaa     1440

gaccucgucg acaaagaaaa gagcgccgag gcuuucauuc auagaaugac gaacaaugau     1500

uuuuaccugc ccgaagagaa agugcugccu aagcacuccc uuaucuauga aaaguucacc     1560

guguacaacg agcucacaaa aguccgcuac aagaaugaac aaggcgagac cuauuucuuc     1620

gacucuaaca ucaaacagga gauuuuugau ggcguuuuca aggaacaucg gaaagugagc     1680

aagaagaaac ugcuggacuu ucucgccaag gaguacgaag aguucagaau cguggauguc     1740

auugggcugg acaaagaaaa uaaggcguuu aacgcuuccc uuggaacaua ucacgaucug     1800

gagaaaaucc ucgacaagga uuuccuggac aaucccgaua acgagagcau ucuggaagac     1860

aucgugcaga cccuuacgcu cuuugaggau cgcgaaauga ucaagaaacg gcuggagaau     1920

uacaaggacc uguucacaga aucucagcuc aaaaagcugu auagacggca cuacaccggc     1980

ugggggcgcc uuuccgccaa acugauuaac ggaaucagag acaaggagag ccaaaagacc     2040

auucucgauu aucugaucga cgauggccgg ucuaauagaa acuuuaugca gcugauuaac     2100

gacgaugggc uuuccuucaa aagcaucauc uccaaggccc aggcuggauc ucauagcgac     2160

aaucucaaag agguuguggg cgaacuggcc ggcuccccgg cuauuaagaa agggauccug     2220

cagucucuca agauugucga ugagcuggug aaaguuaugg gauacgaacc ugagcaaauc     2280

guggucgaga uggcccgcga aaaccagaca accaaucagg gccggagaaa cagccgccag     2340

agauacaaac uucuggacga uggggugaag aaucucgcgu ccgaccugaa cggaaauauu     2400

cugaaggagu aucccacaga uaaccaagcc cuucagaaug aacgccucuu ccuguacuau     2460

cugcagaacg gccgggacau guacacgggg gaggcucucg acaucgauaa ucugagccaa     2520

uaugacaucg aucacauuau cccucaggcc uuuauuaagg acgauucuau cgacaacaga     2580

guccuugugu ccagcgcuaa aaaucggggc aagucugaug acguucccuc ccuggaaauu     2640

gugaaagauu gcaaggucuu cuggaagaaa cuccuggacg ccaagcugau gagccagcgc     2700

aaauacgaua accuuaccaa ggcggagaga ggaggccuga caucugacga uaaagcccgg     2760

uuuauccagc gccaacucgu ggagaccaga cagauuacaa agcauguugc ucggauccug     2820

gacgaacgcu ucaauaacga gcucgacagc aaggggagac ggaucagaaa agugaagauu     2880

gucacccuga aauccaaccu ugugagcaau uuucgcaagg aauuuggauu uuauaaaauc     2940

cgggagguga acaauuacca ccaugcccac gaugcuuacc ugaacgccgu ugucgccaag     3000

gcuauucuca cgaaguaucc ccagcuggaa ccugaguucg uguacggcga cuauccgaaa     3060

uacaauucuu auaagaccag aaaauccgcg acagagaagc uguuuuucua cagcaacauc     3120

augaacuuuu ucaaaaccaa ggugacacuu gccgauggga ccgucguggu uaaggacgau     3180

auugaaguga auaaugacac gggagagauc gucugggaua aaaagaaaca cuucgcuaca     3240

gugcgcaagg uucuguccua uccccagaac aauaucguga aaaagaccga aauucaaaca     3300

ggcggguuuu cuaaagagag cauccucgcc cauggcaacu ccgacaagcu gauuccucgg     3360

aagaccaaag auaucuaccu cgaccccaag aaauacggag gcuucgauuc uccuauuguc     3420

gccuauagcg ugcugguugu ggcugacauc aagaaaggga aggcccagaa acuuaagacc     3480

gucacagaac ugcucggaau cacgauuaug gagagauccc gcuuugagaa gaaucccucu     3540

gcguuccugg aaagcaaagg cuaccugaac auccgggaug acaaacuuau gauucugccg     3600

aaguauuccc ucuuugagcu ggaaaauggg agacgccggc uccuggcuag cgccggagag     3660

cuucagaagg gcaacgagcu ggcccugccu acccaguuca ugaaguuucu cuaccuggcu     3720

ucuagauaua acgaauccaa agggaagccc gaggaaaucg agaaaaagca agaguucgug     3780

aaucagcacg ugagcuacuu ugacgauauu cuucagcuga ucaacgacuu cucuaaacgg     3840

gucauucucg ccgaugcgaa ucuggagaag aucaacaagc ucuaucaaga caauaaagag     3900

aacaucuccg uugaugaacu ggcuaauaac auuaucaauc uuuuuacauu caccagccug     3960

ggcgccccug ccgcuuuuaa guucuucgac aaaauugugg aucgcaagag auacacaucu     4020

accaaagagg ugcugaacuc cacgcucauc caucagagca uuacaggacu guacgaaacc     4080

cggaucgacc uuggcaagcu gggggaggau uga                                  4113


<210>  4293
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4293
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcu cugcuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acaaugcaag aggaaaagua cuaucaugag aaauucccca ccaucuacca ucugcggaag      420

gaacuggcug acaagaaaga gaaggccgau cuccgccuga uuuaccuugc ucuggcccac      480

aucauuaaau uuagaggaca cuuccucauc gaggacgauc gguuugacgu ccgcaacaca      540

gacauucaga agcaguauca ggcguuccug gaaaucuuug auaccacguu cgagaauaac      600

gaccuucugu cccaaaaugu ggauguugaa gccauccuca ccgacaaaau uucuaagagc      660

gcuaaaaagg auagaauccu ggcccgguac ccuaaccaga aguccacagg cauuuuugcu      720

gaguuccuga aacucaucgu gggcaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacac cccuucaguu cgcgaaagac agcuaugaug aggaccugga gaaucuccug      840

gggcaaauug gagaugaauu ugccgaccuu uucucugcug ccaagaagcu guacgacucc      900

guccuccuga gcggcauccu gaccgugacc gaucucucua cgaaagcucc ucuguccgcc      960

agcaugaucc agagauauga cgagcaccgc gaagaucuua agcagcugaa acaguuuguu     1020

aaggccucuc uccccgagaa auaccaagaa auuuucgcug acuccagcaa ggauggguau     1080

gcgggauaca ucgagggcaa gacaaaccag gaggccuuuu acaaauaucu guccaagcug     1140

cugaccaaac aggaaggguc ugaguaccuc cuggaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaaa gaacauuuga uaacggaagc aucccucacc aggugcaucu caccgaacug     1260

cgcgcuauua uccggagaca guccgaguau uacccgcuuc ugaaggagaa ccucgaccgc     1320

aucgaaaaga uucugaccuu ccggaucccc uauuacgucg gcccucuggc cagagagaag     1380

uccgauuuug ccuggaugac acgcaaaacg gacgauucua uucggcccug gaauuucgaa     1440

gaccuugugg auaaggagaa auccgcugag gccuuuaucc acagaaugac caacaaugac     1500

cucuaucugc cugaagagaa ggugcugccc aaacauagcc ucauuuacga aaaguucaca     1560

gucuacaacg agcugaccaa gguucgcuuc cuugcggaag gcuuuaaaga cuuccaguuu     1620

cugaaucgga agcaaaaaga gacaaucuuc aacucucucu uuaaggagaa aagaaaggug     1680

accgaaaagg auauuaucag cuuccugaau aaaguggacg gguaugaggg aaucgcuauu     1740

aagggcaucg aaaaacaguu uaacgccucc cugucuacgu accacgaucu uaagaaaauu     1800

cucgggaagg acuuccugga uaauacagac aacgagcuga uccucgaaga uauuguccag     1860

acccugaccc uuuuugagga ccgggagaug auccgcaaga gacuggauau cuauaaagac     1920

uucuuuacag aaagccagcu caagaaacug uaccggagac acuauaccgg auggggccgc     1980

cuguccgcca aacuuauuaa ugggauccgg aacaaagaga aucaaaagac aauucucgau     2040

uaccugaucg acgauggaag cgcuaacaga aauuucaugc agcugauuaa ggacgccggc     2100

cucucuuuua aaccgaucau cgacaaggcu cgcacgggcu cccauagcga uaaccugaaa     2160

gaagugguug gggagcuugc cggaucuccu gcgauuaaga aaggcauccu gcagucccuc     2220

aagauugugg acgaacuggu caaagugaug ggguaugagc ccgagcagau cguuguggaa     2280

auggccagag agaaccaaac cacagcuaag ggacugagcc ggucccgcca gagacuuacc     2340

acacuccggg aaucucuggc caaucuuaag agcaacauuc ucgaggagaa aaagccuaaa     2400

uacgucaagg aucaggugga aaaucaccau cuguccgacg aucgccuuuu ccuguacuau     2460

cuccagaacg gcaaagacau guacaccgau gacgagcugg auaucgacaa ucugucucaa     2520

uaugauaucg accacauuau cccccaggcu uucauuaagg acgauagcau cgacaacaga     2580

guccuugugu ccagcgccaa gaaucggggg aaaucugaug acguucccuc cguggaaauu     2640

gucaaggauu gcaaaguguu uuggaagaaa cugcucgacg cgaagcugau gagccagcgc     2700

aaguacgaua accucacgaa agccgagaga ggcggacuga ccucugacga uaaggcucgg     2760

uucauccaac gccagcuugu ugaaacaaga cagauuacca aacauguggc ccggauccug     2820

gacgagagau uuaauaacga gcucgauucc aagggccgcc ggaucagaaa agucaagauu     2880

gugacacuua agagcaaucu ggugucuaac uuccgcaaag aauuuggguu cuauaagauc     2940

cgggagguua auaacuacca ccacgcucau gacgccuauc uuaaugccgu cguggcuaaa     3000

gcgauucuga ccaaguaccc ucagcucgaa ccggaguuug uguacggaga uuaucccaaa     3060

uacaacagcu auaagacgag aaaguccgcc acagaaaaac uguucuuuua cagcaacauc     3120

augaauuucu uuaagaccaa agucacacuc gcugacggca ccgugguugu gaaggacgau     3180

auugagguca acaaugacac cggggagauc gugugggaua aaaagaaaca cuucgccaca     3240

guucgcaagg ugcugucuua uccucaaguc aacaucguga agaaaacgga aauucagacc     3300

ggaggcuucu ccaaggagag cauccuugcc caugggaaua gcgacaaacu gauuccccgg     3360

aagacaaaag auaucuaccu cgacccuaag aaguauggcg gauuugauuc ucccauuguu     3420

gcuuacagcg ugcuggucgu ggccgacauc aaaaagggca aagcgcagaa gcugaaaacc     3480

gugacagaac uucuggggau caccauuaug gagagauccc gcuucgaaaa gaacccgucu     3540

gcuuuucucg agagcaaggg auaccuggau auccgggacg auaaacucau gauucugccu     3600

aaguauuccc uuuucgagcu ggaaaauggc agacggcgcc ugcucgccuc ugccggggag     3660

cugcagaaag gaaacgaacu ugcucugccc acgcaauuua ugaaguuccu cuaccuggcc     3720

agcagauaua augagcucaa aggcaagccu gaggaaaucg agcagaagca ggaauuuguc     3780

guucagcacg uguccuacuu cgacgauauu cugcaaauca uuaacgacuu uagcaaucgg     3840

gugauccuug cggacgcuaa ccuggagaaa aucaauaagc uguaucagga uaacaaagaa     3900

aauauucccg ucgacgagcu cgccaacaac aucauuaauc uguucacauu uaccucucuu     3960

ggggccccug cugccuucaa guuuuucgau aaaaucgugg accgcaagag auacaccucc     4020

acaaaagagg uucugaacag cacccucauu caccagucua ucacaggccu guaugaaacg     4080

cggaucgauc ucggaaagcu gggcgaggac uga                                  4113


<210>  4294
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4294
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

guuacagaug acuauaaggu gccugcuaaa aagaugagag uguucggcga uacggaccgg      120

ucucacauca aaaagaaucu ccuggggacc cuucuguuug augacggaaa cacagccgag      180

agccgcagac ucaagcggac cgcgcgcaga cgguacacac gcagacggaa ucgcauucug      240

uaucuucagg aaaucuucac cgaguccaug aacgaaauug augagagcuu uuuccauaga      300

cuggacgaua gcuuucucgu cccggaagac aaacggggcu ccaaguaccc caucuucgcu      360

acacugcaag aggagaaaga auaucacaag caguuuccua ccaucuacca ucugcgcaaa      420

cagcucgccg auucuaagga gaagucugac gugagacuga uuuaucuugc ccuggcucac      480

augaucaaau accgggggca uuuccucuac gaagagacgu uugauauuaa gaauaacgac      540

auccagaaaa uuuucaauga guuuaucaac auuuaugaca auaccuucga aggauccagc      600

cugucuggcc aaaacgccca gguugaggcu aucuuuacag auaagaucuc caaaagcgcc      660

aagcgcgaaa gagugcuuaa acuguucccc gacgagaagu cuaccggccu cuucuccgaa      720

uuucugaagc ugauugucgg gaaucaggcg gauuucaaaa agcacuuuga ccucgaggag      780

aaagccccuc ugcaguucag caaggauaca uacgacgaag aacuugaaaa ccugcucgga      840

caaaucggcg augacuuugc ugaucuguuc cuuauugcca aaaagcugua ugacgcuauc      900

cuccugucug ggauucugac cgugacggau cccuccacaa aggccccucu cagcgcgucc      960

augaucgagc gguacgaaaa ucaccagaaa gaccuggccg cucuuaagca guuuaucaaa     1020

aacaaucugc cggagaagua uguugaggug uucucugacc agagcaaaga uggauacgcc     1080

ggcuauauug acgggaagac cacacaagag gcuuuuuaca aguacaucaa aaaccuccug     1140

uccaaguucg agggagccga uuauuuucuu gacaaaauug aaagagagga uuuccugcgc     1200

aagcagcgga ccuuugacaa cggcagcauc ccccaucaga uucaccucca agaaaugaau     1260

gccauccuga gacgccaggg cgagcauuac ccuuuccuga aagagaaccg ggaaaagauc     1320

gagaagauuc ucaccuucag aauccccuau uacgucgggc cucuggcucg cgauaaucgg     1380

gacuuuucuu ggcuuacaag aaacuccgau gaacccauuc gcccguggaa uuucgaggag     1440

gugguggaca aagcgcggag cgccgaagau uuuauccaca gaaugacgaa cuaugaccug     1500

uaccucccug aggaaaaggu ccugcccaaa cacucucugc uuuaugagac cuucgcuguu     1560

uacaaugaac ucacaaaggu gaaauuuauu gccgagggac ugcgcgauua ccaguuccug     1620

gacagcggcc agaagaaaca aaucgugaac cagcucuuua aggagaagcg gaaagucacc     1680

gaaaaggaca uuauccauua ucugcacaau guggaugggu acgacggaau cgagcuuaaa     1740

ggcauugaaa agcaguucaa cgccaaucug uccacauauc augaucuccu gaaaaucacc     1800

aaggacaagg aguuuaugga ugacccuaaa aacgaagaga uucuggagaa uaucguucac     1860

acgcuuacaa uuuucgaaga uagagagaug aucaagcagc ggcucgcuca auacgacacc     1920

cuguuugaug aaaaagugau caaggcccug acccgcagac auuauacagg guggggaaaa     1980

cucucugcga agcugauuaa cggcauccgg gacaagcaga gcggcaaaac cauucuugau     2040

uaccugaucg acgaugacaa gauuaauaga aacuucaugc agcucaucaa cgacgauggg     2100

cuguccuuua aagagaucau ucagaaggcu caagucgugg gaaaaacaga cgauguuaaa     2160

cagguggucc aggaacugcc cggcagcccc gccaucaaga aagggauucu ucagucuauc     2220

aagauugugg acgagcucgu caaagugaug ggauaugccc uggaguccau cguuaucgaa     2280

auggcucgcg agaaucaaac gaccgcccgg ggcaagaaaa acagccagca gagauacaag     2340

cgcauugaag augcucugaa aaaucucgcc ccugggcugg acucuaacau ccuuaaggag     2400

aauccgacag auaacauuca acugaagaau gacagacucu uccuguacua ucugcagaac     2460

ggcaaagaua uguacaccgg aaagccccuu gacaucaauc agcucuccag cuaugauauu     2520

gaccacauca uuccucaggc guucaucaaa gacgauucca ucgacaaccg ggugcugaca     2580

ucuagcaagg auaaucgcgg caaauccgac aacguccccu cucuggaggu gguucaaaag     2640

agaaaggccu uuuggcagca gcuccuggau agcaaacuua uuuccgaacg gaaguucaau     2700

aaccugacca aagcugagcg cgggggacuc gacgaaagag auaagguggg cuuuauccgg     2760

cgccagcugg ucgagacgca acagauuacc aaaaaugugg cccagauccu ggacgcuaga     2820

uucaacacag aagugaagga gaagaaccag aaaauucgga ccguuaagau caucacacuu     2880

aaaagcaauc uggucucuaa cuuucgcaag gaguucgggc ucuacaaagu gagagaaauu     2940

aauaacuauc accaugccca cgaugcguac cugaaugccg uggucgcuaa ggccauccuc     3000

aagaaauauc cuaagcugga gcccgaauuu guguacggag acuaccaaaa auaugaucuu     3060

aagaaauaca uuucccgguu caagccgagc aaggagaucg aaaaagcuac cgagaaguac     3120

uuuuucuauu cuaaccugcu caacuuuuuc aaagaggaag uucuguaugc cgacggcacg     3180

auuagaaagc gcgagaauau cgaauacucc aaagauacag gggagaucgc cugggacaag     3240

gaaaaagacu ucgcuaccau uaagaaggug cugagcuauc cucaggucaa uaucgugaaa     3300

aagcgggagg uucagacagg cggauuuucu aaagagagca uucuucccaa gggcaacucc     3360

gauaaacuga ucccuagaaa gaccaaggac auucucuggg auaccacaaa auacggggga     3420

uucgacagcc ccgugaucgc guacucuauu cugcucaucg ccgauaucga aaagggcaaa     3480

gcuaagaaac ugaagacggu caagacccuu guggggauua caaucaugga gaaagccgcc     3540

uuugaagaga auccuauuac cuuccuggag aacaagggau aucauaaugu ucgcaaagaa     3600

aacauccucu gccugcccaa guacucccug uuugagcuug aaaauggccg gagacgccug     3660

cucgcuagcg ccaaagagcu gcaaaagggg aacgaaauug ugcucccggu cugucugaca     3720

acccuucugu aucacuccaa gaaccugcac aaacucgacg agccugagca ucuggaauac     3780

auccagaagc accggaauga guucaaagau cuucugaacc ucgugucuga auuuagccag     3840

aaguauaucc uggcggaggc uaaucucgaa aagauuaagg accuguacgc cgauaacgag     3900

caggccgaca ucgagauucu ugcuaauucc uucaucaacc ugcugacguu uacagcccuc     3960

ggcgcgcccg cugccuucaa auuuuucgga aaggaugugg acagaaagcg cuauaccacc     4020

gucucugaaa uucugaaugc cacacuuauc caucaaagca ucaccggccu guacgagaca     4080

cggauugauc ucuccaaacu gggggaagag uga                                  4113


<210>  4295
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4295
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

aucacagaug acuauaaggu uccugcuaaa aagaugaaag ugcucggcaa uacggauaag      120

aacuacauua agaaaaaucu gcuuggggcc cuccuguucg acucuggaaa caccgcggag      180

gauagacggc ugaagcgcac agcuagacgg cgcuauacca gacggcgcaa uagacuucug      240

uaccuccagg acaucuuuag cgaugaaaug aacaaagugg acgaguccuu cuuucaccgg      300

cuggaagauu cuuuccuggu cagcgaggac aagcgcggcg aucaucaccc gauuuuuggg      360

aaugugguug acgaagugaa auaucaugag caauucccca caaucuacca ccucagaaag      420

uaucuggccg auaccccuga gaaggccgac cuucggcugg ucuaccucgc ucuggcccau      480

augaucaaau uucgcggaca cuuccuuauu gacgauccca agcuggacgu gaaaaacaca      540

gauucccagg aacucuuuuc ucaguuccug accgcuuacg acguuacgca ggaggauagc      600

caccuggccg acgugaagau caauauuucc gauaucauua ccgaaaagau gaacaagagc      660

agaaaagucg aggcgcuccu guuucauuuc ccuucucaaa agaaaaauuc cuuuuucggc      720

aaccuuguga gccugguguu cggccuccug cccaauuuua aaacaaacuu caaucuugcc      780

gaggacgcua agcugcagcu cucuaaagau uccuaugacg aagaucugga gaaccugcuc      840

gggcagaucg gagacgaaua caccgacguc uuuguugccg cucggaagcu guaugauagc      900

auucuucugu cuggcauccu cacagugaac gaccugucca ccaaagccau ccuuagcgcg      960

uccauggugc gcagauacga ggaacacaau aaggaucugg ccaagcucaa ggcuuucguc     1020

aaggccaacu uuacgcauga cguguucaaa gagguguuuu cugaugcuag caagaauggg     1080

uaugccggau acauugaggg caaaacaucc caggaagacu ucuauaagua cguuaagaac     1140

cugaucggga aagucgaugg aagcgccgac uuuauugaga agaucgaucg ggaagacuuc     1200

cugagaaaac aacgcaccuu ugauaauggc ucuauuccuc accaggugca ccucgcugag     1260

augaaggcga uccugcggag acagggcgaa uacuauccgu uccuuaaaga gcaugccgag     1320

aagauccagc aaauucugac auuucgcauc cccuacuaug uugggccucu cgaccggcac     1380

ggacauuccg auuucgcuug ggccagcuuc aacucugaag agaaggugac ccccuggaau     1440

uuugaagaga uuaucgacaa aaacaagagc gcccaggcuu ucauugaaag aaugaccaau     1500

caggaccugu accugccuaa cgagaaaguc cuucccaagc acuccccgcu cuaugagaaa     1560

uacacagugu auaaugaacu gacgaagauc aaauacguca ccgagacagg caccgccaag     1620

uuuuucgaug cgaaccugaa gcaagaaauc uuugacggac ucuucaaaaa ggagcgcaaa     1680

gugacaaagc ggaaacugcu uaccuuucug gagaagaauu ucgaugaauu uagaauuguu     1740

gacauccagg ggcucgauaa ggagacggaa acauucaacg cuucuuacgg cauuuaucag     1800

gaccugcuga aaaucauuaa ggauaaagcc uuuauggacg auagcgagaa ugccgacauc     1860

cuugaagaua uugugcucac ccugacccug uucgaggacc gcgagaugau caagcagcgg     1920

cucgcuaaau acgccgaucu guuugacaag aaggucaucg acaaccuugc uagacgccgg     1980

uauacagggu ggggaagacu guccgccaaa cuccugaacg gcauucggga uaagcgcagc     2040

agaaaaacca ucauggacua ccugguggau gacggccggu cuaauagaaa ccuuaugcaa     2100

cucauuacag augacaaucu gacguucaag gaugaaaucg cgaaagccca guauuccgac     2160

aacagcgaug accugcauca gguugugcag gaucucgcug ggucuccugc cauuaagaag     2220

ggaauccugc aauccgucaa aaucguggac gagcuuguga agguuauggg cuacgaaccc     2280

gagcagauug ucguggaaau ggcucgcgag aaucagacca cagccaaagg gcggagaaac     2340

agccagcaac gcuauaagag acuggaggaa gcgaucaaaa aucucgaccg ggaucugaac     2400

uacaagauuc ugaaagagca cccuaccgac aaucaggccc uucagaacga ucgccucuuc     2460

cuguacuauc ugcagaaugg aagagacaug uacacaggcg aaucccucga uaucaaccgg     2520

cugucugacu augauauuga ccacaucauc ccccaggcuu uuauuaagga uaauagcauc     2580

gacaaccgcg ugcuugucuc cucugccaag aauagaggga aaagcgauga cgugcccucc     2640

gaggauguug ugaaccggau gcgcccuuuc uggaacaagc ugcucaauug cggccugauu     2700

agccaaagaa aauacucuaa ccugaccaag aaagaacuua cgccgaauga caaggcugga     2760

uuuaucaagc ggcagcuggu cgagacccgc caaauuacaa aacauguggc cagaauccuc     2820

gacgagcacu ucaacaagga auuugaugag gacaaaaagc ggaucagaaa aguuaaggug     2880

gucacccuga aguccaaucu cgugagcaac uuccgcaaag aauuugagcu guauaagguu     2940

cgggagauua augauuacca ucacgcccac gacgcuuauc uuaacgcggu ggucgccaaa     3000

gcucugcuca ugaaguaccc caaacuggaa ccugaguucg uguacggcga auaucccaag     3060

uacaauucuu auagagagcg caagacagcc acccagcgga uguuuuucua cuccaacauc     3120

augaauaugu uuaaaagcaa ggugaaacug gccgaugaaa gaauuaucga gcgcccuaug     3180

auugagguca acgacgaaac gggggagauc gcuugggaua agacaaaaca uauugccacc     3240

guuaagaagg ugcuuucuua uccccagauc aauaucguga aaaaggucga ggaacagaca     3300

ggaggccuga ccaacgaaac cauuuacccg cgggggagcu ucgacaaacu ccugaauaga     3360

aagaacacac ucgaggcguc caaauaugga ggcuucggga gcccuacggu ggcuuacugu     3420

guucuggugg ucgccgauuc uaagaacgag aauggcaaag ugaagaaacu uaaacuggga     3480

uccgaacucc ugggcaucac cauucuggag aagaacgacu uugaaaauaa cagccuugau     3540

uuccuguccc gcaaagggua ccaaggaguu caggagaaga aaaucauuaa gcuccccaag     3600

uauucucugu uugaguucca ggacggcagc cggagaaugc ucuccucugu gauggccaau     3660

gauaacaaac gcggggaccu gcagaaggcu aaugaaauga uccuuccugc cuaccugguc     3720

aaacugcucu aucacgcgaa gcauaucaac agcgugggag agcccgcuca caaaaauuac     3780

auuaacgaac auugcuccga guuucaagac cugcuuaauu auauccggca guucgccgau     3840

aaguuuguug acguggauaa gaaccugucu aaaauuagaa gccucuccaa gagcauggcc     3900

gaauacucug ucuccgagau cuguagcucu uucgugaauc ugcucacacu gaccgcucuu     3960

ggcgccccug cugacuuuaa auucuuuggg acaaccauuc cccggaagcg cuauggcucc     4020

ccgcagagca uccugucugc cacgcugauc caccagucca uuacaggacu cuacgagacc     4080

agaaucgauc ugagcaaacu uggcgaagac uga                                  4113


<210>  4296
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4296
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcu cugcuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acaaugcaag aggaaaagua cuaucaugag aaauucccca ccaucuacca ucugcggaag      420

gaacuggcug acaagaaaga gaaggccgau cuccgccuga uuuaccuugc ucuggcccac      480

aucauuaaau uuagaggaca cuuccucauc gaggacgauc gguuugacgu ccgcaacaca      540

gacauucaga agcaguauca ggcguuccug gaaaucuuug auaccacguu cgagaauaac      600

gaccuucugu cccaaaaugu ggauguugaa gccauccuca ccgacaaaau uucuaagagc      660

gcuaaaaagg auagaauccu ggcccgguac ccuaaccaga aguccacagg cauuuuugcu      720

gaguuccuga aacucaucgu gggcaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacac cccuucaguu cgcgaaagac agcuaugaug aggaccugga gaaucuccug      840

gggcaaauug gagaugaauu ugccgaccuu uucucugcug ccaagaagcu guacgacucc      900

guccuccuga gcggcauccu gaccgugacc gaucucucua cgaaagcucc ucuguccgcc      960

agcaugaucc agagauauga cgagcaccgc gaagaucuua agcagcugaa acaguuuguu     1020

aaggccucuc uccccgagaa auaccaagaa auuuucgcug acuccagcaa ggauggguau     1080

gcgggauaca ucgagggcaa gacaaaccag gaggccuuuu acaaauaucu guccaaacuu     1140

cugaccaaac aggaaggguc ugaguaccuc cuggaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaaa gaacauuuga uaacggaagc aucccucacc aggugcaucu caccgaacug     1260

cgcgcuauua uccggagaca guccgaguau uacccguucc uuaaggagaa ccuggaccgc     1320

aucgaaaaga uucucaccuu ucggaucccc uauuacgucg gcccucuggc cagagaaaag     1380

agcgauuucg ccuggaugac acgcaaaacg gacgauucua uucggcccug gaauuuugaa     1440

gaccuggugg auaaggagaa auccgcugag gccuucaucc acagaaugac caacaaugac     1500

cuuuaucucc cugaagagaa ggugcugccc aaacauagcc ugauuuacga aaaguucaca     1560

gucuacaacg agcucaccaa gguucgcuuu cuggcggaag gcuucaaaga cuuucaguuc     1620

cuuaaucgga agcaaaaaga gacaaucuuu aacucucugu ucaaggagaa aagaaaggug     1680

accgaaaagg auauuaucag cuuucucaau aaaguggacg gguaugaggg aaucgcuauu     1740

aagggcaucg aaaaacaguu caacgccucc cugucuacgu accacgaucu gaagaaaauu     1800

cuugggaagg acuuucucga uaauacagac aacgagcuga uccuggaaga uauuguccag     1860

acccucaccc uguucgagga ccgggagaug auccgcaaga gacuugauau cuauaaagac     1920

uuuuucacag aaagccagcu gaagaaacuc uaccggagac acuauaccgg auggggccgc     1980

cuguccgcca agcugauuaa ugggauccgg aacaaagaga aucaaaagac aauucuugau     2040

uaccucaucg acgauggaag cgcuaacaga aauuuuaugc agcugauuaa ggacgccggc     2100

cugucuuuca aaccgaucau cgacaaggcu cgcacgggcu cccauagcga uaaccucaaa     2160

gaagugguug gggagcuggc cggaucuccu gcgauuaaga aaggcauccu ucagucccug     2220

aagauugugg acgaacucgu caaagugaug ggguaugagc ccgagcagau cguuguggaa     2280

auggccagag agaaccaaac cacagcuaag ggacugagcc ggcugcgcca gagacuuacc     2340

acacuccggg aaucccuggc caaucugaag ucuaacauuc ucgaggagaa aaagccuaaa     2400

uacgucaagg aucaggugga aaaucaccau cugagcgacg aucgccuuuu ccuguacuau     2460

cuccagaacg gcaaagacau guacaccgau gacgagcugg auaucgacaa ucugucccaa     2520

uaugauaucg accacauuau cccccaggcu uuuauuaagg acgauucuau cgacaacaga     2580

guccuuguga gcuccgccaa gaaucggggg aaaagcgaug acguucccuc uguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa cugcucgacg cgaagcugau gucccagcgc     2700

aaguacgaua accucacgaa agccgagaga ggcggacuga ccagcgacga uaaggcucgg     2760

uuuauccaac gccagcuugu ugaaacaaga cagauuacca aacauguggc ccggauccug     2820

gacgagagau ucaauaacga gcucgauucu aagggccgcc ggaucagaaa agucaagauu     2880

gugacacuga aguccaaucu ggugagcaac uuucgcaaag aauuuggguu uuauaagauc     2940

cgggagguua auaacuacca ccacgcucau gacgccuauc uuaaugccgu cguggcuaaa     3000

gcgauucuga ccaaguaccc ucagcucgaa ccggaguucg uguacggaga uuaucccaaa     3060

uacaacucuu auaagacgag aaagagcgcc acagaaaaac uguuuuucua cuccaacauc     3120

augaauuucu ucaagaccaa agucacacuc gcugacggca ccgugguugu gaaggacgau     3180

auugagguca acaaugacac cggggagauc gugugggaua aaaagaaaca cuucgccaca     3240

guucgcaagg ugcugagcua uccucaaguc aacaucguga agaaaacgga aauucagacc     3300

ggaggcuuuu cuaaggaguc cauccuugcc caugggaaua gcgacaaacu gauuccccgg     3360

aagacaaaag auaucuaccu cgacccuaag aaguauggcg gauucgauuc ccccauuguu     3420

gcuuacucug ugcuggucgu ggccgacauc aaaaagggca aagcgcagaa gcugaaaacc     3480

gugacagaac uucuggggau caccauuaug gagagaagcc gcuuugaaaa gaacccgucc     3540

gcuuuccucg agucuaaggg auaccugaau auccgggaug acaaacucau gauucugccu     3600

aaguauagcc uuuuugagcu ggaaaacggc agacggcgcc ugcucgccuc cgccgaugag     3660

cugcagaaag ggaaugaacu ugcucugccc acgcaauuca ugaaguuucu cuaccuggcc     3720

ucuagauaua acgagcucaa aggaaagccu gaggaaaucg agcagaagca ggaguucguc     3780

guucagcacg ugagcuacuu ugacgauauu cugcaaauca uuaaugacuu cuccaaccgg     3840

gugauccuug cggacgcuaa ucuggagaaa aucaacaagc uguaucagga uaauaaagaa     3900

aacauucccg ucgacgagcu cgccaacaau aucauuaacc uguuuacauu caccagccuu     3960

ggcgccccug cugccuuuaa guucuucgau aaaaucgugg accgcaagag auacaccucu     4020

acaaaagagg uucuuaauuc cacccucauu caccagagca ucacagggcu guaugaaacg     4080

cggaucgauc ucggcaagcu gggagaggac uga                                  4113


<210>  4297
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4297
auggagaaga acaaagaaua cgugaucggc cuggacauug ggaccggaag cgucggcugg       60

uccgccaucu cuacagauaa uuaucagcuc auuagacaaa aggggaaaaa ccugaucgga      120

guucggcuuu ucaauagcgc ugagacggcc gaagaacgca gaggcuaccg gaccacacgc      180

agacggcugu cccgcagacg guggcgcaug ucucuccuga acgacauuuu uagacccgaa      240

cuuaaugaga ucgaugagaa cuuccuggcg cggcucaagu auagcugggu guccccucug      300

gaccagaaaa auccgcagcu gaacgaacag gggaccaaug cuucucucua cggagauauc      360

gaggccgacc aagccuuuca ccgcgcuuau cccacaauuu accaucugag aaagacccuu      420

auggaagaua accagaagca cgaccugcgg gagguguauc ucgccaucca ucacauuguc      480

aaauaccgcg gccauuuccu gacaccugau uuugacgcua auagccagac caucgauauu      540

aagggccuuu ucaaacagcu gggggacgug uccggaagca ucgagcucca agccgaucag      600

acgucuaccg uuauugcggc cuuucagucc auccugcugg acuucacaaa gacccccaca      660

gaaagcggca gacucgcugg gcagcuguuu ucugaccaaa acaaagauaa gcaggccaag      720

aaacaggugg aggaacuuuu caagcugcuc cggggaggca aagggucccu ggcugacaug      780

uuucuucuga ccgaucucga caaggucgug aaaaaucaac ugaaguucgc cuuugaugac      840

gcgguugaug agaaccugaa ucucaucagc aaccugauua acgaggagca guacgagcuu      900

cugcucaaua uccagaaacu gagaggaacg auuauccuua agcagauucu gggcgacgau      960

gaaacagugu cugacgccaa gguccgcucc uaugaggaac acgcucacca acucaaagag     1020

cugaaggccc aggugcugac caaagauaac aaggaaaaau ucgaggaggc uuaccagcuc     1080

auccagaagg gcgacgccag ccggaucgac gggcugaaga aacuuaagac aauuaucaau     1140

caagaacaug aggcccugag acucgauaaa uaugaagcug acaagcugcu gaaacuuauu     1200

gagcucgauc agcugcugcc ugugcagcgc uccaagcgga acggagaaau cccccaccag     1260

cuccaugagu ucgagcugaa gaaaauuauc gaaaagcaag gcagauacua uccuuuucuu     1320

cgcgagaccu ucgaacggga cgggcaggag uuuaauaaaa ucggacagcu ggucaacuuc     1380

cgcauuccgu acuauguugg cccccucgug gauccuagac uggcggggau ccagucugga     1440

gaccaaacca aucacuggau gacacagaag aaagccggca agauuacgcc cuggaacuac     1500

accgagcuga ucgauauuga caagagcgcu gccgaauuua ucaaacggau gacaggcacc     1560

gauacauacc uuguggggga gccuucccuc cacaaggcca gccugauuua ucaggacuac     1620

aauguccugc aagaacucaa caaucugaga uaugauggaa aaccccuucc gguggacgcu     1680

aagcaggaga ucuacucuaa ccugcagguu ucccucaaaa augccaccaa gaacaaaauc     1740

aauagcuucc ugauuucuga aggcuaucug caggaugggg aggagcuuac gggauuugcg     1800

ggcaaggaac ucaagaacaa acugagcucc uuccauuacu uuaagaacau cuucgggccu     1860

gagcgcauga auacacuggg caccgacaac cucgagaaaa uuaccgagcu gcaaacagug     1920

uuugaagacc ggcacaugcu uaagagacag cugcagaaac ucggauuccu ggaugacgau     1980

gagugcgaga agcuggcugg caagcauuuu accggguggg gaaaucuuuc uaaaaagcuc     2040

cugaacacac ccgccaucau ugacccugaa aucaaugaga acaaaaucag ccugauugau     2100

gcccucuaua agggccagcg gaaucugucc uucuacguca acaauaugga cgauaaauac     2160

caauuccgcg acuggaucga aagcgagaac cagucucaac agguggaaau uucccuugau     2220

gagaagauca agaaucuggc ugggagcccc gccauuaaaa gaggaaucac gcagucuaug     2280

gcuguuaucc aggacauuau ccgggccaac aauggcauuu cccccaagag aaucuaucuc     2340

gaguuugcgc gcgaaagcca gaacuccuac caaccuaauu cucggauuaa acagaucgag     2400

aagaucuaug aucagaacaa aaaggaacug augaaggugu acccggacgu cuucaaagau     2460

gugcaggggg uccugaacaa uguggacgag aacaauagcc uuuccgagaa ggccuuucug     2520

uauuucaugc aacucggcaa agacauguac accggagaag cucuggauuu ugacaagcuc     2580

uauacauacg auauugacca caucauuccc caggccauga ccaaagauaa cucuugggac     2640

aauaagguuc uggugacagc uaucaaaaac agagaaaaga gcaaucaggu ccuugauaac     2700

gccauucagg cgaagaugca uaauuucugg cugcaacuca aaaaggugaa ccugcugucc     2760

cagcgcaaau acgacacccu uaugaagacg aauuuuagca aaggcgauga aaagaaguuc     2820

aucgccagau cucugguuga gacccggcag auuaucaaaa acguggcuga acucaucaag     2880

uccgaguuug accacgucga ggccauuccu auccgccagg aacugacaag cgauaugaga     2940

caacucaugg acuauaaaaa gaaucggcug gugaacgacu accaccaugc ucacgaugcc     3000

cuucugauug ccaccguggg ggaguucaug cagaaacgcg gauuuaugca gggcaugaau     3060

cccucucaga acguuuauca uggguacaca caauauguca agaaguggcu cuccauggcu     3120

aaaaaggcga gaagcgccga aaaaccugag cggaucuucc cccugcaguu ugugguggga     3180

ucuaugcgca uggcugucag agacggccag guggauaagg uuacccaccc ggccacgggg     3240

gaaauuuguu ggacagccga caacgaggcu uaccugcgga aagugcuugg cuauaagaau     3300

auccugguca cccgcaaggu gaaaaacgcc ggaaagcucu accaaagagu uacagugaaa     3360

ccuaagggca gcggggaggc gcccgucaau caccagaaau ccgauaucaa cauuuacgga     3420

ggcgugacca gcgaacaggc ugccgagcug guucucaucc ggauuaaggc caagaauaac     3480

gcuaaaaaua uggugaucaa gauuccuacc gccaucgcga accaggucgc uaauaaaaca     3540

acgaccuaug acgccuacgu gagaucucug aacaucccca auuaugccgg gauuauccuu     3600

gaucaaauuc cucugggaca gcugguguuc gacgaacgca cauccuucua ccucgcuagc     3660

gccgagauca agcauaacgc ucggcagcug ugggucaauc agcacauuaa caauguucuu     3720

accaaaaaga acaucaauga ugaagagaga gacaacauuc ugaaccuccu gacaaaugag     3780

aaaaaccucg aacgcuuuaa gcugcuugag cccgaucuga agcgggugaa agccgcgaag     3840

caacaguggg ccuccugcga cgaucaggaa cagaugcaaa uccugagaau cauuaucaau     3900

aaaaugcaug acgacgcuuc uccgcagauu ccuggcaaga ucgaucccuu caaaaacuau     3960

cucgacaagc aggggaaggg cuggcgcgau cugcggacca aaaagggaau gaaacuuagc     4020

gacaaugccg aguuugugua ccaauccccc acgggccugu aucacagaaa gauuucuauc     4080

aacgagcucc ugaaacagaa gcagcucgaa uga                                  4113


<210>  4298
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4298
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaggu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcc cuucuguucg acggaggcaa caccgcugcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acaaugcaag aggaaaagua cuaucaugag aaauucccca ccaucuacca ucugcggaag      420

gaacuggcug acaagaaaga gaaggccgau cuccgccuga uuuaccuugc ucuggcccac      480

aucauuaaau uuagaggaca cuuccucauc gaggacgauc gguuugacgu ccgcaacaca      540

gacauucaga agcaguauca ggcguuccug gaaaucuuug auaccacguu cgagaauaac      600

gaccuucugu cccaaaaugu ggauguugaa gccauccuca ccgacaaaau uucuaagagc      660

gcuaaaaagg auagaauccu ggcccgguac ccuaaccaga aguccacagg cauuuuugcu      720

gaguuccuga aacucaucgu gggcaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacac cccuucaguu cgcgaaagac agcuaugaug aggaccugga gaaucuccug      840

gggcaaauug gagaugaauu ugccgaccuu uucucugcug ccaagaagcu guacgacucc      900

guccuccuga gcggcauccu gaccgugacc gaucucucua cgaaagcucc ucuguccgcc      960

agcaugaucc agagauauga cgagcaccgc gaagaucuua agcagcugaa acaguuuguu     1020

aaggccucuc uccccgagaa auaccaagaa auuuucgcug acuccagcaa ggauggguau     1080

gcgggauaca ucgagggcaa gacaaaccag gaggccuuuu acaaauaucu guccaaacuu     1140

cugaccaaac aggaaggguc ugaguaccuc cuggaaaaga uuaaaaauga ggacuuccug     1200

cggaagcaaa gaacauuuga uaacggaagc aucccucacc aggugcaucu caccgaacug     1260

cgcgcuauua uccggagaca guccgaguau uacccguucc uuaaggagaa ccuggaccgc     1320

aucgaaaaga uucucaccuu ucggaucccc uauuacgucg gcccucuggc cagagaaaag     1380

agcgauuucg ccuggaugac acgcaaaacg gacgauucua uucggcccug gaauuuugaa     1440

gaccuggugg auaaggagaa auccgcugag gccuucaucc acagaaugac caacaaugac     1500

cuuuaucucc cugaagagaa ggugcugccc aaacauagcc ugauuuacga aaaguucaca     1560

gucuacaacg agcucaccaa gguucgcuuu cuggcggaag gcuucaaaga cuuucaguuc     1620

cuuaaucgga agcaaaaaga gacaaucuuu aacucucugu ucaaggagaa aagaaaggug     1680

accgaaaagg auauuaucag cuuucucaau aaaguggacg gguaugaggg aaucgcuauu     1740

aagggcaucg aaaaacaguu caacgccucc cugucuacgu accacgaucu gaagaaaauu     1800

cuugggaagg acuuucucga uaauacagac aacgagcuga uccuggaaga uauuguccag     1860

acccucaccc uguucgagga ccgggagaug auccgcaaga gacuugauau cuauaaagac     1920

uuuuucacag aaagccagcu gaagaaacuc uaccggagac acuauaccgg auggggccgc     1980

cuguccgcca agcugauuaa ugggauccgg aacaaagaga aucaaaagac aauucuugau     2040

uaccucaucg acgauggaag cgcuaacaga aauuuuaugc agcugauuaa ggacgccggc     2100

cugucuuuca aaccgaucau cgacaaggcu cgcacgggcu cccaucucga uaaccugaaa     2160

gaagugguug gggagcuugc cggaagcccu gcgauuaaga aaggcauccu gcagucucuc     2220

aagauugugg acgaacuggu caaagugaug ggguaugagc ccgagcagau cguuguggaa     2280

auggccagag agaaccaaac cacagcuaag ggacuguccc ggagccgcca gagacuuacc     2340

acacuccggg aaucccuggc caaucugaag ucuaacauuc ucgaggagaa aaagccuaaa     2400

uacgucaagg aucaggugga aaaucaccau cugagcgacg aucgccuuuu ccuguacuau     2460

cuccagaacg gcaaagacau guacaccgau gacgagcugg auaucgacaa ucugucccaa     2520

uaugauaucg accacauuau cccccaggcu uuuauuaagg acgauucuau cgacaacaga     2580

guccuuguga gcuccgccaa gaaucggggg aaaagcgaug acguucccuc ucuggaaauu     2640

gugaaggauu gcaaagucuu cuggaagaaa cuccuggacg cgaagcucau gucccagcgc     2700

aaguacgaua accugacgaa agccgagaga ggcggacuua ccagcgacga uaaggcucgg     2760

uuuauccaac gccagcuggu ggaaacaaga cagauuacca aacauguugc ccggauccuc     2820

gacgagagau ucaauaacga gcuggauucu aagggccgcc ggaucagaaa agugaagauu     2880

gucacacuga aguccaaucu ugugagcaac uuucgcaaag aguucggguu uuauaagauc     2940

cgggagguga auaacuacca ccacgcucau gacgccuauc ugaaugccgu ugucgcuaaa     3000

gcgauucuca ccaaguaccc ucagcuggaa ccggaguucg uguacggaga uuaucccaaa     3060

uacaacucuu auaagacgag aaagagcgcc acagaaaaac ucuuuuucua cuccaacauc     3120

augaacuuuu ucaagaccaa agugacacug gcugacggca ccgucguggu uaaggacgau     3180

auugagguga auaaugacac cggggagauc gucugggaua aaaagaaaca cuucgccaca     3240

gugcgcaagg uucuuagcua uccucaagug aacaucguca agaaaacgga agugcagacc     3300

ggaggcuuuu cuaaggaguc cauucuggcc caugggaaua gcgacaaacu caucccccgg     3360

aagacaaaag auauuuaccu ggacccuaag aaguauggcg gauucgauuc ccccaucguu     3420

gcuuacucug ugcuggucgu ggccgacauu aaaaagggca aagcgcagaa acuuaagacc     3480

gugacagaac ugcucgggau caccaucaug gagagaagcc gcuuugaaaa gaacccgucc     3540

gcuuuccugg agucuaaggg auaccucaau auucggacgg auaaacugau cauucuuccu     3600

aaguauagcc uguuugagcu ggaaaacggc agacggcgcc uccuggccuc cgccggggag     3660

cuucagaaag gaaaugaacu ggcucucccc acacaauaca ugaaguuccu guaucucgcc     3720

ucuagauaca acgagcugaa aggcaagccu gaggaaaucg agcagaagca ggaauuuguc     3780

guucagcacg ugagcuauuu cgacgauauu cuucaaauca uuaaugacuu uuccaaccgg     3840

gugauccugg cggaugcuaa ucuggagaaa aucaacaagc ucuaccagga caauaaagaa     3900

aacauucccg ucgacgagcu ggccaacaau aucauuaacc uuuucaccuu uaccagccug     3960

ggggccccug cugccuucaa guuuuucgau aaaaucgugg accgcaagag auauacaucu     4020

accaaagagg uucucaauuc cacacugauu caccagagca ucacgggccu cuacgaaacc     4080

cggaucgauc ugggaaaacu uggcgagggg uga                                  4113


<210>  4299
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4299
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcc cuucuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acacugcaag aggaaaagga cuaccaugag aaauucucca ccaucuauca ucugcggaag      420

gaacucgcug auaagaaaga gaaggccgac cugcgccuua uuuacaucgc ucuggcccac      480

auuaucaaau uuagaggaca cuuccucauu gaggaugacu cuuuugacgu ccggaacaca      540

gauaucagca agcaguacca ggacuuccug gaaaucuuua auaccacguu cgagaacaau      600

gaucuucugu cccagaacgu ggacguugaa gcgauucuca ccgauaaaau cagcaagucu      660

gccaaaaagg accgcauucu ggcucaauau cccaaucaga aguccacagg caucuuugcc      720

gaguuccuga aacucauugu gggcaaccag gcugauuuca agaaauacuu uaaucuggaa      780

gacaagaccc cucuucaguu cgccaaagau agcuaugacg aggaucugga gaaccuccug      840

gggcaaaucg gagacgaauu ugcggaccuu uucucugccg cuaagaagcu guacgauucc      900

guccuccuga gcggcauccu gacagugauu gaccucucua ccaaagcucc ccuguccgcu      960

agcaugaucc agagauauga ugagcaucgg gaagaccuua agcagcugaa acaguuuguu     1020

aaggccuccc ucccugagaa auaccaagaa auuuucgccg auucuagcaa ggacggguau     1080

gcuggauaca ucgagggcaa gacgaaucag gaggcguuuu acaaauaucu guccaagcug     1140

cugacaaaac aggaagauag cgagaacuuc cucgaaaaga uuaaaaacga ggacuuucug     1200

agaaagcaac gcaccuucga uaaugggucu aucccccacc aggugcaccu gacagaacuc     1260

aaggccauca uucggagaca guccgaguac uauccuuuuc ugaaagagaa ccaggaccgc     1320

aucgaaaaga uucuuaccuu ccggaucccg uacuauauug gaccccuggc uagagagaaa     1380

agcgauuuug ccuggaugac ccgcaagaca gacgauucua uccggccuug gaauuucgaa     1440

gaccucgucg acaaagaaaa gagcgccgag gcuuucauuc auagaaugac gaacaaugau     1500

uuuuaccugc ccgaagagaa agugcugccu aagcacuccc uuaucuauga aaaguucacc     1560

guguacaacg agcucacaaa aguccgcuac aagaaugaac aaggcgagac cuauuucuuc     1620

gacucuaaca ucaaacagga gauuuuugau ggcguuuuca aggaacaucg gaaagugagc     1680

aagaagaaac ugcuggacuu ucucgccaag gaguacgaag aguucagaau cguggauguc     1740

auugggcugg acaaagaaaa uaaggcguuu aacgcuuccc uuggaacaua ucacgaucug     1800

gagaaaaucc ucgacaagga uuuccuggac aaucccgaua acgagagcau ucuggaagac     1860

aucgugcaga cccuuacgcu cuuugaggau cgcgaaauga ucaagaaacg gcuggagaau     1920

uacaaggacc uguucacaga aucucagcuc aaaaagcugu auagacggca cuacaccggc     1980

ugggggcgcc uuuccgccaa acugauuaac ggaaucagag acaaggagag ccaaaagacc     2040

auucucgauu aucugaucga cgauggccgg ucuaauagaa acuuuaugca gcugauuaac     2100

gacgaugggc uuuccuucaa aagcaucauc uccaaggccc aggcuggauc ucauagcgac     2160

aaucucaaag agguuguggg cgaacuggcc ggcuccccgg cuauuaagaa agggauccug     2220

cagucucuca agauugucga ugagcuggug aaaguuaugg gauacgaacc ugagcaaauc     2280

guggucgaga uggcccgcga aaaccagaca accaaucagg gccggagaaa cagccgccag     2340

agauacaaac uucuggacga uggggugaag aaucucgcgu ccgaccugaa cggaaauauu     2400

cugaaggagu aucccacaga uaaccaagcc cuucagaaug aacgccucuu ccuguacuau     2460

cugcagaacg gccgggacau guacacgggg gaggcucucg acaucgauga ccugagccaa     2520

uaugauaucg accacauuau cccucaggcc uuuauuaagg augacucuau cgauaauaga     2580

guccuugugu ccagcgcuaa aaaccggggc aagucugacg auguucccuc ccuggaaauu     2640

gugaaagacu gcaaggucuu cuggaagaaa cuccuggaug ccaagcugau gagccagcgc     2700

aaauacgaca aucuuaccaa ggcggagaga ggaggccuga caucugauga caaagcccgg     2760

uuuauccagc gccaacucgu ggagaccaga cagauuacaa agcauguugc ucggauccug     2820

gacgaacgcu ucaacaauga gcucgauagc aaggggagac ggaucagaaa agugaagauu     2880

gucacccuga aauccaaccu ugugagcaac uuucgcaagg aauuuggauu uuauaaaauc     2940

cgggagguga auaacuacca ccaugcccac gacgcuuacc ugaaugccgu ugucgccaag     3000

gcuauucuca cgaaguaucc ccagcuggaa ccugaguucg uguacggcga uuauccgaaa     3060

uacaacucuu auaagaccag aaaauccgcg acagagaagc uguuuuucua cagcaauauc     3120

augaacuuuu ucaaaaccaa ggugacacuu gccgacggga ccgucguggu uaaggaugac     3180

auugaaguga auaacgauac gggagagauc gucugggaca aaaagaaaca cuucgcuaca     3240

gugcgcaagg uucuguccua uccccagaau aacaucguga aaaagaccga aauucaaaca     3300

ggcggguuuu cuaaagagag cauccucgcc cauggcaauu ccgauaagcu gauuccucgg     3360

aagaccaaag acaucuaccu cgaucccaag aaauacggag gcuucgacuc uccuauuguc     3420

gccuauagcg ugcugguugu ggcugauauc aagaaaggga aggcccagaa acuuaagacc     3480

gucacagaac ugcucggaau cacgauuaug gagagauccc gcuuugagaa gaaccccucu     3540

gcguuccugg aaagcaaagg cuaccugaau auccgggcug acaaacuuau uauccugccg     3600

aaguauuccc ucuuugagcu ggaaaacggg agacgccggc uccuggccag cgccggagag     3660

cuucagaagg gcaaugagcu ggcucugccu acccaguuca ugaaguuucu cuaccuggcc     3720

ucuagauaua acgaauccaa agggaagccc gaggaaauug agaaaaagca agaguucgug     3780

aaccagcacg ugagcuacuu ugacgauauc cuucagcuga uuaaugacuu cucuaaacgg     3840

gucauccucg cggaugcuaa ccuggagaag aucaauaagc ucuaucaaga caacaaagag     3900

aauauuuccg uugaugaacu ggccaacaau aucauuaacc uuuuuacauu caccagccug     3960

ggcgccccug cugccuuuaa guucuucgac aaaaucgugg aucgcaagag auacacaucu     4020

accaaagagg ugcuuaauuc cacgcucauu caucagagca ucacaggacu guacgaaacc     4080

cggaucgacc uuggcaagcu gggggaggau uga                                  4113


<210>  4300
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4300
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcc cuucuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acacugcaag aggaaaagga cuaccaugag aaauucucca ccaucuauca ucugcggaag      420

gaacucgcug auaagaaaga gcgcgccgac cugagacuua uuuacaucgc ucuggcccac      480

auuaucaagu uucggggaca cuuccucauu gaggaugacu cuuuugacgu ccgcaacaca      540

gauaucagca aacaguacca ggacuuccug gaaaucuuua auaccacguu cgagaacaau      600

gaucuucugu cccagaacgu ggacguugaa gcgauucuca ccgauaagau cagcaaaucu      660

gccaagaaag accggauucu ggcucaauau cccaaucaga aguccacagg caucuuugcc      720

gaguuccuga agcucauugu gggcaaccag gcugauuuca aaaaguacuu uaaucuggaa      780

gacaaaaccc cucuucaguu cgccaaggau agcuaugacg aggaucugga gaaccuccug      840

gggcaaaucg gagacgaauu ugcggaccuu uucucugccg cuaaaaagcu guacgauucc      900

guccuccuga gcggcauccu gacagugauu gaccucucua ccaaggcucc ccuguccgcu      960

agcaugaucc agagauauga ugagcauaga gaagaccuua aacagcugaa gcaguuuguu     1020

aaagccuccc ucccugagaa guaccaagaa auuuucgccg auucuagcaa agacggguau     1080

gcuggauaca ucgagggcaa gacgaaucag gaggcguuuu acaaguaucu guccaaacuu     1140

cugacaaagc aggaagauag cgagaacuuc cucgaaaaga uuaagaacga ggacuuucug     1200

cgcaaacaac ggaccuucga uaaugggucu aucccccacc aggugcaccu gacagaacuc     1260

aaggccauca uuagacgcca guccgaguac uauccuuuuc ugaaggagaa ccaggaccgg     1320

aucgagaaaa uucuuaccuu cagaaucccg uacuauauug gaccccuggc ucgcgaaaag     1380

agcgauuuug ccuggaugac ccggaaaaca gacgauucua ucagaccuug gaauuucgaa     1440

gaccucgucg acaaggagaa aagcgccgag gcuuucauuc aucgcaugac gaacaaugau     1500

uuuuaccugc ccgaagagaa ggugcugccu aaacacuccc uuaucuauga aaaguucacc     1560

guguacaacg agcucacaaa gguccgguac aaaaaugaac aaggcgagac cuauuucuuc     1620

gacucuaaca ucaagcagga gauuuuugau ggcguuuuca aagaacauag aaaggugagc     1680

aaaaagaagc ugcuggacuu ucucgccaaa gaguacgaag aguuccgcau cguggauguc     1740

auugggcugg acaaggaaaa uaaagcguuu aacgcuuccc uuggaacaua ucacgaucug     1800

gagaagaucc ucgacaaaga uuuccuggac aaucccgaua acgagagcau ucuggaagac     1860

aucgugcaga cccuuacgcu cuuugaggau cgggaaauga ucaagaagag acuggagaau     1920

uacaaagacc uguucacaga aucucagcuc aagaaacugu aucggcgcca cuacaccggc     1980

ugggggagac uuuccgccaa gcugauuaac ggaauccggg acaaagagag ccaaaagacc     2040

auucucgauu aucugaucga cgauggcaga ucuaaucgca acuuuaugca gcugauuaac     2100

gacgaugggc uuuccuucaa gagcaucauc uccaaagccc aggcuggauc ucauagcgac     2160

aaucucaagg agguuguggg cgaacuggcc ggcuccccgg cuauuaaaaa ggggauccug     2220

cagucucuca aaauugucga ugagcuggug aagguuaugg gauacgaacc ugagcaaauc     2280

guggucgaga uggcccggga aaaccagaca accaaucagg gcagacgcaa cagcagacag     2340

cgguacaaac uucuggacga uggggugaag aaucucgcgu ccgaccugaa cggaaauauu     2400

cugaaggagu aucccacaga uaaccaagcc cuucagaaug aacgccucuu ccuguacuau     2460

cugcagaacg gcagagacau guacacgggg gaggcucucg acaucgauga ccugagccaa     2520

uaugauaucg accacauuau cccucaggcc uuuauuaaag augacucuau cgauaaucgg     2580

guccuugugu ccagcgcuaa gaaccgcggc aaaucugacg auguucccuc ccuggaaauu     2640

gugaaggacu gcaaagucuu cuggaagaag cuccuggaug ccaaacugau gagccagaga     2700

aaguacgaca aucuuaccaa agcggagcgg ggaggccuga caucugauga caaggcccgc     2760

uuuauccaga gacaacucgu ggagacccgg cagauuacaa aacauguugc ucgcauccug     2820

gacgaaagau ucaacaauga gcucgauagc aaggggcgga gaauccgcaa ggugaaaauu     2880

gucacccuga aguccaaccu ugugagcaac uuucggaaag aguucggauu uuauaagauc     2940

agagaaguga auaacuacca ccaugcccac gacgcuuacc ugaaugccgu ugucgccaaa     3000

gcuauucuca cgaaguaucc ccagcuggaa ccugaguucg uguacggcga uuauccgaag     3060

uacaacucuu auaaaacccg caaguccgcg acagagaaac uguuuuucua cagcaauauc     3120

augaacuuuu ucaagaccaa agugacacuu gccgacggga ccgucguggu uaaggaugac     3180

auugaaguga auaacgauac gggagagauc gucugggaca agaaaaagca cuucgcuaca     3240

gugcggaaag uucuguccua uccccagaau aacaucguga agaaaaccga aauucaaaca     3300

ggcggguuuu cuaaggagag cauccucgcc cauggcaauu ccgauaaacu gauuccuaga     3360

aagaccaagg acaucuaccu cgaucccaaa aaguacggag gcuucgacuc uccuauuguc     3420

gccuauagcg ugcugguugu ggcugauauc aaaaagggga aagcccagaa acuuaagacc     3480

gucacagaac ugcucggaau cacgauuaug gagcgcuccc gguuugagaa aaaccccucu     3540

gcguuccugg aaagcaaggg cuaccugaau aucagagcug acaaacuuau uauccugccg     3600

aaguauuccc ucuuugagcu ggaaaacggg cgccggagac uccuggccag cgccggagag     3660

cuucagaaag gcaaugagcu ggcucugccu acccaguuca ugaaguuucu cuaccuggcc     3720

ucucgguaua acgaauccaa ggggaaaccc gaggaaauug agaagaaaca agaauuugug     3780

aaccagcacg ugagcuacuu ugacgauauc cuucagcuga uuaaugacuu cucuaagcgc     3840

gucauccucg cggaugcuaa ccuggagaaa aucaauaagc ucuaucaaga caacaaggag     3900

aauauuuccg uugaugaacu ggccaacaau aucauuaacc uuuuuacauu caccagccug     3960

ggcgccccug cugccuuuaa auucuucgac aagaucgugg auagaaaacg guacacaucu     4020

accaaggagg ugcucaauuc cacgcugauu caucagagca ucacaggacu guacgaaacc     4080

cgcaucgacc uuggcaaacu gggggaggau uga                                  4113


<210>  4301
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4301
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcu cugcuguucg acggaggcaa caccgccagc      180

gaucggcgcc ucaagagaac agcgcggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc ugaggaaaug uccaaagugg acgauucuuu cuuucaccgc      300

cuggaggaca gcuuccucgu ggaagaugac aagagagggu ccaaauaucc gauuuuugcc      360

acaaugcaag aggagaagga uuaccaugaa aaauucccca ccaucuauca ucugcggaag      420

gagcuggccg acaagaaaga aaaggcugau cuccgccugu uuuaccuugc ccuggcucac      480

auuaucaaau ucagaggaca cuuucucauu gaggacgacu cuuucgaugu ccggaacaca      540

gacauccagc gccaguacca ggccuuucug gagauuuucg auaccacguu ugaaaauaac      600

caucuucuga gccaaaauau cgacguggag ggcauccuca ccgauaagau uuccaaaagc      660

gcgaagaaag acagaauccu ggcccaguau ccuaaccaga agucuacagg cauuuucgcu      720

gaauuucuga agcucaucgu ugggaaucag gccgauuuua aaaagcacuu caaccuggag      780

gacaaaacac cccuucaauu ugcuaaggau uccuacgacg aagaucugga gaaucuccug      840

ggacagauug gcgacgaguu cgccgaccuu uuuagcgugg cgaaaaagcu guaugauucu      900

guccuccugu ccgggauccu gaccgugacc gaccucagca cgaaggcccc ucugucugcu      960

uccaugaucc agcgguacga ugaacacaga gaggaccuua aacagcugaa gcaauucguu     1020

aaagccagcc uccccgaaaa guaucaggag auuuuuacag auuccucuaa agacggauac     1080

gcuggcuaua ucgaagggaa gaccaaccag ggagccuucu acaaguaccu gagcaaacuu     1140

cugacaaagc aagagggcuc cgaguauuuu cucgaaaaga uuaagaauga ggauuuccug     1200

cgcaaacagc ggaccuuuga caacggcagc aucccucauc aggugcaccu gaccgaacuc     1260

aaggccauua ucagacgcca gucugaguac uauccguucc ugaaggaaaa ccuugaucgg     1320

aucgagaaaa uucugacauu uagaaucccc uacuaugucg ggccucucgc ucgcgagaag     1380

uccgacuucg cguggaugac gcggaaaacc gaugacagca uuagacccug gaauuucgag     1440

gaacuggugg auaaggaagc cucugcugag gccuuuaucc aucgcaugac aaacaaugac     1500

cuguaccuuc cugaggaaaa agugcucccc aagcacagcc ugauuuauga gaaauucacc     1560

gucuacaacg aacugacaaa gguucgguac aagaaugagc aaggagaaac cuauuucuuc     1620

gacuccaaca ucaaacagga gauuuuugau ggcguguuca aggagcacag aaaagugucu     1680

aagaaaaagc uccuggacuu ucuugccaag gaauacgagg aguuccgcau cgucgaugug     1740

aucgggcugg acaaagaaaa uaaggcuuuu aacgccagcc ucggaacgua ucaugaucug     1800

aaaaagauuc uggacaaaga uuuccuugac aauccggaua acgaauccau ccucgaggac     1860

auuguucaga cacugacccu guuugaggau cgggaaauga ucaagaagag acucgagaau     1920

uacaaagacc uguucaccga aagccagcuu aagaaacugu aucggcgcca cuacacaggc     1980

ugggggagac ucucugcgaa gcugauuaac ggaauccggg auaaagaguc ccaaaagacc     2040

auccuggacu aucuuauuga cgauggcaag agcaauagaa acuuuaugca gcucauccau     2100

gacgauggcc ugucuuucaa auccauuauc agcaaggcuc aggccggguc ccacucugac     2160

aaucugaaag aaguggucgg agagcucgcc ggcagcccug cuauuaagaa agggauccug     2220

cagucccuua agaucgugga ugagcugguu aaagugaugg gauacgaacc cgagcaaauu     2280

gucguggaaa uggcccgcga gaaccagaca acgaaccagg gccggagaaa uucucgccag     2340

agauacaagc uccuggacga uggggucaag aaccuggcua gcgaccuuaa uggcaacauc     2400

cucaaagagu auccuaccga uaaucaagcc cugcagaacg aacggcuguu ccucuacuau     2460

cugcagaaug gacgcgacau guacacaggc aaggcgcuug auauugacaa ccugucccaa     2520

uaugacaucg aucauauuau cccccaggcc uuuaucaaag acgauagcau ugacaauaga     2580

gugcucguuu cuuccgcuaa gaaccggggg aaaagcgaug acgugcccuc ucuggagauc     2640

gucaaggauu gcaagguguu cuggaaaaag cugcuugacg ccaaacugau gucccagcgc     2700

aaguacgaua aucucaccaa agcugaaaga ggaggccuga caagcgacga uaaggcccgg     2760

uuuauucagc gccaacucgu ugagaccaga cagaucacga agcacguggc gcggauucug     2820

gacgaacgcu ucaacaauga gcuugauucu aaagggagac ggaucagaaa ggucaaaauu     2880

gugacccuua agagcaaccu cguguccaau uuucgcaaag aguucggauu uuauaagauc     2940

cgggaaguua acaacuacca ccaugcccac gacgcuuacc ugaaugccgu cguggcuaag     3000

gccauccuga caaaauaucc ucagcuugag ccggaguucg uguacggcga cuaucccaag     3060

uacaacagcu auaaaaccag aaagucugcc acagaaaaac uguuuuucua cuccaauauu     3120

augaacuuuu ucaagaccaa ggucacgcuc gcugauggga cagugguugu gaaagacgau     3180

aucgaaguca auaacgacac cggcgagauu gugugggaua agaaaaagca uuucgcgaca     3240

guucgcaaag ugcugagcua uccucagguc aauaucguga agaaaaccga gauucaaacc     3300

ggaggcuuuu ccaaggaauc uauccucgcc cacgggaaca gcgacaagcu gaucccccgg     3360

aaaacaaagg auauuuaccu ugacccuaaa aaguacggag gcuucgauuc ccccaucguu     3420

gcuuauucug ugcuggucgu ggccgacauu aaaaagggga aggcccagaa acucaagacg     3480

gugaccgagc ugcugggaau cacaauuaug gaaagaagcc gcuuugagaa aaauccgucc     3540

gcuuuccuug aaucuaaggg cuaccugaac auccgggaug acaaacucau gauccugccu     3600

aaguauagcc ucuuugagcu ggagaauggg agacgccggc uucuggccuc cgcgggcgaa     3660

cugcagaagg gaaacgagcu cgcucugccc acccaguuca ugaaauuucu uuaccuggcc     3720

agcagauaua augaacucaa gggcaaaccu gaggagauug aacaaaagca ggaguucguc     3780

guucagcacg ugucuuacuu ugaugacauc cugcaaauua ucaacgacuu cuccaaucgg     3840

gugauucucg ccgaugcuaa ccuggagaaa aucaacaaac uuuaucagga caauaaggaa     3900

aacauuagcg ucgaugaacu ggccaauaac aucaucaauc uguuuacauu caccucucuc     3960

ggggcgcccg cugccuuuaa auucuucgac aagauugugg aucgcaaaag auacacgucc     4020

acaaaggagg uucugaacag cacccuuauc caucagucua uuaccggacu guacgagaca     4080

cggaucgacc ucggcaaacu gggggaagau uga                                  4113


<210>  4302
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4302
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcu cugcuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acaaugcaag aggaaaagua cuaucaugag aaauucccca ccaucuacca ucugcggaag      420

gaacuggcug acaagaaaga gaaggccgau cuccgccuga uuuaccuugc ucuggcccac      480

aucauuaaau uuagaggaca cuuccucauc gaggacgauc gguuugacgu ccgcaacaca      540

gacauucaga agcaguauca ggcguuccug gaaaucuuug auaccacguu cgagaauaac      600

gaccuucugu cccaaaaugu ggauguugaa gccauccuca ccgacaaaau uucuaagagc      660

gcuaaaaagg auagaauccu ggcccgguac ccuaaccaga aguccacagg cauuuuugcu      720

gaguuccuga aacucaucgu gggcaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacac cccuucaguu cgcgaaagac agcuaugaug aggaccugga gaaucuccug      840

gggcaaauug gagaugaauu ugccgaccuu uucucugcug ccaagaagcu guacgacucc      900

guccuccuga gcggcauccu gaccgugacc gaucucucua cgaaagcucc ucuguccgcc      960

agcaugaucc agcacuauga cgagcacaga gaagaucuua agcagcugaa acaguuuguu     1020

aaggccucuc uccccgagaa auaccaagaa auuuucgcug acuccagcaa ggauggguau     1080

gcgggauaca ucgagggcaa gacaaaccag gaggccuuuu acaaauaucu guccaaacuu     1140

cugaccaaac aggaaggguc ugaguaccuc cuggaaaaga uuaaaaauga ggacuuccug     1200

cgcaagcaac ggacauuuga uaacggaagc aucccucauc aggugcaccu caccgaacug     1260

agagcuauua uccgccggca guccgaguau uacccguucc uuaaggagaa ccuggacaga     1320

aucgaaaaga uucucaccuu ucgcaucccc uauuacgucg gcccucuggc ccgggaaaag     1380

agcgauuucg ccuggaugac aagaaaaacg gacgauucua uucgccccug gaauuuugaa     1440

gaccuggugg auaaggagaa auccgcugag gccuucaucc aucggaugac caacaaugac     1500

cuuuaucucc cugaagagaa ggugcugccc aaacacagcc ugauuuacga aaaguucaca     1560

gucuacaacg agcucaccaa gguuagauuu cuggcggaag gcuucaaaga cuuucaguuc     1620

cuuaaucgca agcaaaaaga gacaaucuuu aacucucugu ucaaggagaa acggaaggug     1680

accgaaaagg auauuaucag cuuucucaau aaaguggacg gguaugaggg aaucgcuauu     1740

aagggcaucg aaaaacaguu caacgccucc cugucuacgu accacgaucu gaagaaaauu     1800

cuugggaagg acuuucucga uaauacagac aacgagcuga uccuggaaga uauuguccag     1860

acccucaccc uguucgagga cagagagaug auccggaagc gccuugauau cuauaaagac     1920

uuuuucacag aaagccagcu gaagaaacuc uacagacggc auuauaccgg auggggcaga     1980

cuguccgcca agcugauuaa ugggauccgc aacaaagaga aucaaaagac aauucuugau     2040

uaccucaucg acgauggaag cgcuaaccgg aauuuuaugc agcugauuaa ggacgccggc     2100

cugucuuuca aaccgaucau cgacaaggcu agaacgggcu cccacagcga uaaccucaaa     2160

gaagugguug gggagcuggc cggaucuccu gcgauuaaga aaggcauccu ucagucccug     2220

aagauugugg acgaacucgu caaagugaug ggguaugagc ccgagcagau cguuguggaa     2280

auggcccgcg agaaccaaac cacagcuaag ggacugagca gaucccggca gcgccugacc     2340

acacuuagag aaucucucgc caaucuuaag agcaacauuc uggaggagaa aaagccuaaa     2400

uacgucaagg aucaggugga aaaucaucac cucuccgacg aucggcuguu ccuuuacuau     2460

cugcagaacg gcaaagacau guacaccgau gacgagcucg auaucgacaa ucugucucaa     2520

uaugauaucg accauauuau cccccaggcu uuuauuaagg acgauagcau cgacaaccgc     2580

guccuggugu ccagcgccaa gaauagaggg aaaucugaug acguucccuc cguggaaauu     2640

gucaaggauu gcaaaguguu cuggaagaaa cuucuggacg cgaagcucau gagccagcgg     2700

aaguacgaua accugacgaa agccgagcgc ggcggacuca ccucugacga uaaggcuaga     2760

uuuauccaac ggcagcuggu ugaaacacgc cagauuacca aacacguggc cagaauccuu     2820

gacgagcggu ucaauaacga gcuggauucc aagggcagac gcauccggaa agucaagauu     2880

gugacacuca agagcaaucu ggugucuaac uuuagaaaag aauuuggguu uuauaagauc     2940

cgcgagguua auaacuacca ccaugcucac gacgccuauc ugaaugccgu cguggcuaaa     3000

gcgauucuua ccaaguaccc ucagcuggaa ccggaguucg uguacggaga uuaucccaaa     3060

uacaacagcu auaagacgcg gaaguccgcc acagaaaaac ucuuuuucua cagcaacauc     3120

augaauuucu ucaagaccaa agucacacug gcugacggca ccgugguugu gaaggacgau     3180

auugagguca acaaugacac cggggagauc gugugggaua aaaagaaaca uuucgccaca     3240

guuagaaagg ugcucucuua uccucaaguc aacaucguga agaaaacgga aauucagacc     3300

ggaggcuuuu ccaaggagag cauccuggcc cacggcaauu ccgacaaacu uauuccccgc     3360

aagacaaaag auaucuaccu ggacccuaag aaguaugggg gauucgauuc ucccauuguu     3420

gcuuacagcg ugcucgucgu ggccgacauc aaaaagggca aagcgcagaa gcugaaaacc     3480

gugacagaac ugcuugggau caccauuaug gagcggucca gauuugaaaa gaacccgucu     3540

gcuuuccugg agagcaaggg auaccucaau auccgcgaug acaaacugau gauucucccu     3600

aaguauuccc uguuugagcu ugaaaacggc cggagacggc ugcuggccuc ugccggggag     3660

cuccagaaag gaaaugaacu ggcccuuccc acgcaauuca ugaaguuucu guaccucgcu     3720

agccgcuaua acgagcugaa aggcaagccu gaggaaaucg agcagaagca ggaguucguc     3780

guucagcacg uguccuacuu ugaugacauu cuccaaauca uuaaugauuu cagcaacaga     3840

gugauccugg cggacgcuaa ucuugagaaa aucaacaagc uguaucagga caauaaagaa     3900

aacauucccg ucgaugagcu ggccaacaau aucauuaacc ucuuuacauu caccucucug     3960

ggggccccug cugccuuuaa guucuucgac aaaaucgugg aucggaagcg cuacaccucc     4020

acaaaagagg uucuuaauag cacccugauu caucagucua ucacaggccu cuaugaaacg     4080

agaaucgacc ugggaaagcu cggcgaggau uga                                  4113


<210>  4303
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4303
augaagguga acaauuacca caucggccug gacauuggga ccagcuccau cggauggguc       60

gccauuggcg aggaugggaa accccucaga aucaagggaa aaacagcuau uggcgcccgg      120

cuguuccagg aagggaaccc ugcggcugac cgcagaaugu uucggacgac ccgcagacgg      180

cuuucucgca gaaaguggcg gcugaaacuc cuggaggaaa ucuucgaucc guauaucaca      240

cccguugaca gcaccuuuuu cgcccgccuu aagcaaucca aucugucucc uaaggauagc      300

agaaaagagu uuaagggauc caugcuuuuc cccgaccuga cagauaugca guaccauaaa      360

gacuauccua ccauuuacca ucugcggcac gcccucauga cacaggauga aaaguuugac      420

auccgcaugg uguaucuggc uauucaccau aucgugaaau acagaggcaa cuuccuuaau      480

ucuacccccg ucgauagcuu uaaggccucc aaggugaacu ucguugacca guuuaaaaag      540

cugaaugagc ucuauacggc uauuaacccu gaggaauccu uccaaaucaa ucuggccaac      600

ucugaggaua uuggccacca guuucuugac ccguccaucc ggaaauucga caagaaaaag      660

cagaucccca aaauugugcc ugucagcgug gaugacaagg cgaccgauaa gaucaauggg      720

aaaauugccu cugaaaucau uaacgcuauc cugggauaca aguccaaacu cgacguugug      780

guccagugca cacccgugga uagcaagucu ugggcccuga aauucgacga ggaggauauc      840

gacgcuaagc ugcaaaagau ucucccugaa auggaugaga aucagcaguc caucauugcc      900

auccugcaga accuuuacag ccaagugacc cugaaucaga uuguccccaa cggcaugucc      960

cucucugaaa gcaugaucga gaaauauaac gaccaccaug aucaccugaa gcucuacaaa     1020

aagaucauug accagcuggc ggacccgaaa aagaaggccg cucucaaaaa ggccuauucc     1080

caauacguug gggaugacgg aaaagugauc gaacaggcug auuuuuggag cucugugaag     1140

aaaaaucugg acgauuccga ccugagcaag cagauuaugg aucucaucga cgccgagaag     1200

uucaugccua aacagcgcac aucucaaaac ggcgucauuc cccaucagcu gcaccagaga     1260

gagcuugaug aaaucauuga gcaccagagc aaguauuacc cuuggcuggc cgaaaucaau     1320

cccaacaaac augaccucca ccuggcuaag uauaaaaucg aggaacuugu ggcguuucgg     1380

guucccuacu acguggggcc uaugauuacc ccggaugacc aagccaaguc cgcugagacg     1440

gucuucucuu ggauggagag aaaaggaaag gaagccggcc agaucacacc cuggaauuuu     1500

gaugagaagg uggaccgcaa cgccagcgcu aaucgguuca uuaaaagaau gaccacaaag     1560

gacaccuauc ugaucggcga agauguucuc ccugacgagu cccugcugua cgaaaaauuu     1620

aaggugcuca acgagcugaa uaugguccgc gugaacggga aacuucugaa ggucgccgau     1680

aagcaggcga uuuuccagga ccucuuugag aauuauaaac auaucagcgu gaagaaacug     1740

caaaacuaca ucaagucuaa aaccggacug cccuccgauc cugaaauuag cggccuuucu     1800

gaccccgagu auuucaauaa cucccucggg acauacaaug auuuuaagaa gcuguucgga     1860

aacaaaguug acgaaccgga ucugcaggac gauuuugaga agaucgugga guggagcacg     1920

gucuucgaag acaaacggau ucucagagag aagcugaacg aaaucaccug gcuuuccgau     1980

cagcagaaag acgugcugga gucuagccgc uaucaaggcu gggggcggcu cuccaagaag     2040

cugcugacag gaauuguuaa ugaccagggc gaaagaauca ucgaugagcu uuggaacacc     2100

aauaaaaacu ucaugcagau ucaaucugac aaugauuuug cuaagcgcau ccacgaggcc     2160

aacgccgacc agaugaaagc uguggauguc gaagacgugc ucgccgaugc uuacacaagc     2220

ccucagaaua agaaagccau uagacaggug guuaaggucg uggacgauau ccaaaaagcg     2280

augggcgggg uggcccccaa guacauuucc aucgaguuca cccggagcga agaccgcaac     2340

ccucggagaa cgaucucucg gcagcgccag cuggagaaua cacugaagga uaccgcuaaa     2400

ucccucgcca agagcauuaa ccccgaacug cuuucugagc uggacaaugc ugccaaaucc     2460

aagaaaggac ucaccgacag acuguaucug uacuuuacac agcuuggcaa ggauaucuau     2520

accgggaagc cuauuaacau cgacgagauu agcacauacg auaucgacca uauucucccc     2580

caagcguucg ucaaagauga cucucugaau aaccgggugc ugguuagcaa ggccaucaau     2640

aacggaaaau ccgauaacgu gccgguccag cucuuuggcg cuaagauggg gcacuucugg     2700

aaacagcugg ccgaagcugg ccuuaucagc aagagaaagc ugaaaaaucu ccagacggac     2760

ccugauacca uuucuaagua ugccaugcac ggauuuaucc gccggcaacu gguggagaca     2820

ucccagguua uuaaacuggu ggccaacauc cuuggcgaca aguacagaaa ugauaacacc     2880

aaaauuaucg aaaucacagc ucgcaugaau caucagauga gagacgaguu cggguuuauu     2940

aagaaccggg aaaucaauga uuaucaccau gcguucgacg ccuaccucac cgcuuuucug     3000

ggacgcuacc uguaucacag auacauuaag cuccggcccu auuucgucua cggcgacuuu     3060

aaaaaguuca aagaggauaa ggugacgaug cgcaacuuca auuuucugca cgaccuuacc     3120

gaugacacac aagagaaaau cgccgaugcc gaaaccgggg agguuauuug ggacagagaa     3180

aacagcaucc agcagcugaa ggauguguau cauuacaagu ucaugcucau cucccacgag     3240

gucuauacac ugcggggagc uauguuuaau cagaccgugu accccgccuc ugacgcgggc     3300

aaacgcaagc ugauuccuau caaagcugau agaccgauua acguguacgg gggcuauagc     3360

ggauccgccg acgccuacau ggcuaucguc cggauucaua auaagaaagg cgauaaguau     3420

cgcguugugg gggugcccau gagagcccgg gacagacuug augcggcuaa aaaggucucu     3480

gacgccgacu gugaucgcgc ccugaaggac gugcucacgc cucaacugac aaaaaccaag     3540

aaaagccgga agacaggaga gaucacccag guuguggaag auuucgagau uguccucggc     3600

aaagugaugu acagacagcu gaugaucgac ggggauaaga aguuuaugcu uggauccucu     3660

accuaucagu acaacgcuaa acaacugguu cucagcgacc aguccgugaa gacacuggcc     3720

agcaaaggcc gccuggaucc ccuucaggaa ucuauggacu auaauaacgu cuacacggag     3780

auccuggaua aggugaacca guacuucucc cucuaugaca ugaauaaauu ucggcacaag     3840

cugaaccucg gguucagcaa guuuauuucu uucccuaauc auaacguuuu ugauggcaau     3900

accaaagcua gcagcggaaa gagagaaauc cuggaggagg ugcuuaacgg ccugcacgcc     3960

aaucccacau ucgggaaccu caaagacauu ggaaucacca caccuuucgg ccaacugcag     4020

cagcccaaug ggauucugcu uucugacgaa gcgaagaucc gcuaccaguc cccgaccgga     4080

cuguuugagc ggacggucag ccucaaagau cuguga                               4116


<210>  4304
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4304
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug auuccggaga gaccgccgaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuaga      240

uaucugcagg agaucuucuc uagcgaaaug uccaaagugg acgauuccuu uuuccaucgg      300

cuugaggaga gcuuucuggu cgaagaggac aagaaacacg aacgccaucc uaucuucggc      360

aacauugugg augagguugc cuaccacgaa aaguauccga ccaucuacca ucucagaaag      420

aaacuggccg acuccacaga uaaggcugac cugcggcuca uuuaucuggc ccuugcucac      480

augaucaaau uucgcgggca cuuccugauu gagggagauc ucaaucccga caacagcgac      540

auggauaagc uguuuaucca acuugugcag accuacaauc agcuguucga ggaaaacccu      600

auuaaugccu cuagagucga cgcgaaagcc auccucuccg cucggcugag caagucucgc      660

agacuggaga accucaucgc ccagcugccc ggcgaaaaac ggaauggccu uuuugggaac      720

cugauugcuc ucucccuggg acuuacgccu aauuucaaga gcaacuuuga ucuggccgag      780

gacgcgaagc uccaacuguc uaaagauacc uacgacgaug accuggauaa ucuccuggcc      840

cagaucggcg accaguaugc ugaucuuuuc cuggccgcua agaaccucuc cgacgccauu      900

cugcuuagcg acauccugag agugaacucc gaaauuacaa aagcuccccu cucugcuagc      960

augaucaagc gcuacgauga gcaucaccag gaccugaccc ugcucaaagc gcugguucgg     1020

caacagcuuc cugagaagua uaaggaaauc uucuuugauc aguccaaaaa uggguacgcc     1080

ggauauauug acggcggggc uagccaggag gaguucuaca aguuuaucaa accgauucug     1140

gaaaagaugg auggaacaga ggaacuccug gccaaacuga acagagagga ccuucuccgc     1200

aagcaacgga ccuucgauaa uggcucuauc ccccaucaga uucaccuggg cgaacugcac     1260

gccauccuca gacgccagga ggacuuuuau ccuuuccuga aggauaaccg ggaaaagauc     1320

gagaagauuc uuacguuuag aauccccuac uacguggggc cucuggcucg cggaaauucc     1380

cgguucgccu ggaugacaag aaaaagcgaa gagaccauua cacccuggaa cuuugaggaa     1440

gucguggaca agggcgcguc ugcucagagc uucaucgagc gcaugaccaa uuuugauaaa     1500

aaccucccga augaaaaggu gcugccuaag cauucccugc uuuaugagua cuucaccguc     1560

uauaacgagc ucacaaaagu uaaguacgug acggaaggga ugcggaaacc cgaguuucug     1620

ucuggaaagc aaaaagaagc cauuguggac cuccuguuca agaccaauag aaaagucaca     1680

gugaagcagc ugaaggagga uuauuucaaa aagaucgaau gcuuugacag cguugagauu     1740

uccggcgugg aggaccgguu caacgccagc cuugggaccu accacgaucu gcucaaaauc     1800

aucaaggaca aagauuuucu ggacaaugaa gagaacgaag auauucugga ggacaucguc     1860

cuuacacuca cccuguucga agauaaggag augauugagg aacgccugaa gaaauaugcu     1920

aaucucuuug acgauaaggu gaugaaacag cugaagagac ggcauuacac gggauggggc     1980

agacuuucuc gcaaacugau caacggcauu cgggacaagc aauccgggaa gacaauccuc     2040

gauuuccuga aaagcgacgg auuugccaac agaaauuuca ugcagcugau caacgaugac     2100

ucucuuaccu uuaaggaggc uauucagaaa gcccagguuu ccggccaagg gcacagccuc     2160

caugaacaga ucgcgaaucu ggccggaucc ccugcuauua agaaaggcau ccugcagucu     2220

gugaaggucg uggacgagcu cgucaaggug auggggcaca aacccgaaaa cauuguuauc     2280

gagauggccc gcgagaauca gaccacacaa aagggccaga aaaacagcag agaacggaug     2340

aagcgcaucg aggaaggaau uaaagagcug ggcucccaga uccuuaagga gcaccccgug     2400

gaaaauaccc agcugcaaaa cgagaaacuc uaccuguauu accugcagaa ugggagagau     2460

auguaugucg accaggaacu ugauauuaac cggcugucug acuacgaugu ggaccauauc     2520

guuccucaga gcuucauuaa ggaugacucc aucgauaaua aggugcucac acgcagcgac     2580

aaaaacagag gaaagucuga uaaugucccg uccgaggaag uggugaaaaa gaugaaaaac     2640

uauuggcggc aacugcucaa ugcuaagcug aucacgcagc gcaaguuuga caaccuuacc     2700

aaagccgaga gaggcgggcu gagcgagcuc gacaaggcgg gauucauuaa acggcagcug     2760

guugaaacac gccaaaucac caagcacguc gcccagauuc uggauucuag aaugaacaca     2820

aaauacgacg agaaugauaa acuuauccgg gaagugaagg ugauuacccu gaaauccaag     2880

cucgucagcg acuuuagaaa agauuuccag uucuauaagg ugcgcgagau caacaauuac     2940

caucacgcuc acgacgccua ccugaacgcu guugugggca cggcccucau uaaaaaguau     3000

cccaagcugg aaucugaguu ugucuacggg gauuauaaag uguacgacgu ucggaagaug     3060

aucgccaaaa gcgagcagga aaucggcaag gcuaccgcga aaagauucuu uuauuccaau     3120

auuaugaacu ucuuuaagac agagaucacc cuugccaaug gagaaauucg caagcggccu     3180

cugaucgaga caaacgaaga gaccggcgag auuguguggg auaaagggag agacuucgcu     3240

acgguccgca aggugcucag caugccccaa guuaauaucg ugaaaaagac agaaguccag     3300

accggagccc ugacaaacga gucuaucuac gcccggggcu ccuuugauaa acugauuagc     3360

agaaagcauc gcuucgaauc cucuaaauau gggggauuug gcagcccuac cgugaccuac     3420

uccgugcuug ucguugcuaa gucuaaggug caggacggga aagugaagaa aaucaagaca     3480

ggcaaagagc ugauuggaau cacgcuccug gauaagcucg ucuucgagaa gaauccccug     3540

aaauuuauug aagacaaggg cuacgggaac gugcagaucg acaaaugcau caaacuuccg     3600

aaauauagcc uguucgaguu cgaaaaugga acccggagaa ugcuggccuc cguuauggcg     3660

aacaauaacu cucggggcga ucuccaaaag gcuaacgaga uguuucugcc ugccaaacuu     3720

gugacacugc ucuaccacgc ccauaagauu gaaagcucca aggagcugga gcacgaggcu     3780

uauauccucg accauuacaa ugaucuguau cagcuucuga gcuacauuga acgcuucgcc     3840

ucucuguaug ucgacgugga aaagaacauc uccaagguua aagagcucuu uagcaauauu     3900

gaaucuuacu ccaucagcga gauuuguucu uccgugauca accugcuuac ccugacagcg     3960

agcggggcuc ccgccgauuu caaguuucuc ggaaccacga ucccuagaaa gcgguacggc     4020

ucuccccaga gcauucuguc uagcacacuc auccaccaau ccauuaccgg gcuguaugag     4080

acccgcaucg accuuucuca gcugggcgga gauuga                               4116


<210>  4305
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4305
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug auuccggaga gaccgccgaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuaga      240

uaucugcagg agaucuucuc uagcgaaaug uccaaagugg acgauagcuu uuuccaucgg      300

cuugaggaau cuuuucuggu cgaggaggac aagaaacacg aacgccaucc uaucuucggc      360

aacauugugg augagguugc cuaccacgaa aaguauccga ccaucuacca ucucagaaag      420

aaacuggccg acuccacaga uaaggcugac cugcggcuca uuuaucuggc ccuugcucac      480

augaucaaau uucgcgggca cuuccugauu gagggagauc ucaaucccga caacagcgac      540

auggauaagc uguuuaucca acuugugcag accuacaauc agcuguucga ggaaaacccu      600

auuaaugccu cuagagucga cgcgaaagcc auccucuccg cucggcugag caagucucgc      660

agacuggaga accucaucgc ccagcugccc ggcgaaaaac ggaauggccu uuuugggaac      720

cugauugcuc ucucccuggg acuuacgccu aauuucaaga gcaacuuuga ucuggccgag      780

gacgcgaagc uccaacuguc uaaagauacc uacgacgaug accuggauaa ucuccuggcc      840

cagaucggcg accaguaugc ugaucuuuuc cuggccgcua agaaccucuc cgacgccauu      900

cugcuuagcg acauccugag agugaacucc gaaauuacaa aagcuccccu cucugcuagc      960

augaucaagc gcuacgauga gcaucaccag gaccugaccc ugcucaaagc gcugguucgg     1020

caacagcuuc cugagaagua uaaggaaauc uucuuugauc aguccaaaaa uggguacgcc     1080

ggauauauug acggcggggc uagccaggag gaguucuaca aguuuaucaa accgauucug     1140

gaaaagaugg auggaacaga ggaacuccug gccaaacuga acagagagga ccuucuccgc     1200

aagcaacgga ccuucgauaa uggcucuauc ccccaucaga uucaccuggg cgaacugcac     1260

gccauccuca gacgccagga ggacuuuuau ccuuuccuga aggauaaccg ggaaaagauc     1320

gagaagauuc uuacguuuag aauccccuac uacguggggc cucuggcucg cggaaauucc     1380

cgguucgccu ggaugacaag aaaaagcgaa gagaccauua cacccuggaa cuuugaggaa     1440

gucguggaca agggcgcguc ugcucaaucc uucaucgagc gcaugaccaa uuuugauaaa     1500

aaccucccga augaaaaggu gcugccuaag cauucccugc uuuaugagua cuucaccguc     1560

uauaacgagc ucacaaaagu uaaguacgug acggaaggga ugcggaaacc cgaguuucug     1620

ucuggaaagc agaaagaagc cauuguggac cuccuguuca agaccaauag aaaagucaca     1680

gugaagcagc ugaaggagga uuauuucaaa aagaucgaau gcuuugacag cguugagauu     1740

uccggcgugg aggaccgguu caacgccagc cuugggaccu accacgaucu gcucaaaauc     1800

aucaaggaca aagauuuucu ggacaaugaa gagaacgaag auauucugga ggacaucguc     1860

cuuacacuca cccuguucga agauaaggag augauugagg aacgccugaa gacguaugcu     1920

caucucuuug acgauaaagu gaugaagcag cugaaaagac ggcacuacac aggauggggc     1980

agacuuucuc gcaagcugau caauggcauu cgggacaaac aauccgggaa gaccauccuc     2040

gauuuccuua agagcgacgg auuugccaac agaaacuuca uccagcugau ucaugaugac     2100

ucucugaccu uuaaagaggc uauccagaag gcccagguuu ccggccaagg gcacagccuc     2160

cacgaacaga uugcgaaucu ggccggaucc ccugcuauca aaaagggcau ucugcagucu     2220

gugaaagucg uggacgagcu cgucaaggug auggggcaua agcccgaaaa caucguuauc     2280

gagauggccc gcgagaauca gacaacccaa aaaggccaga agaacagcag agaacggaug     2340

aaacgcauug aggaaggaau caaggagcug ggcucccaga uucuuaaaga gcaccccgug     2400

gaaaauacac agcugcaaaa cgagaagcuc uaccuguauu accugcagaa ugggagagau     2460

auguaugucg accaggaacu ugauaucaac cggcugucug acuacgaugu ggaccauauu     2520

guuccucaga gcuucaucaa agaugacucc aucgauaaua aggugcucac gcgcagcgac     2580

aagaacagag gaaaaucuga ugacgucccg uccgaggaag uggugaagaa aaugaagaau     2640

uauuggcggc aacugcucaa cgcuaaacug auuacccagc gcaaguuuga caaucuuaca     2700

aaggccgaga gaggcgggcu gagcgagcuc gauaaagcgg gauucaucaa gcggcagcug     2760

guugaaaccc gccaaauuac aaaacacguc gcccagaucc uggacucuag aaugaacacc     2820

aaguacgaug agaaugacaa acuuauucgg gaagugaagg ugaucacgcu gaaguccaaa     2880

cucgucagcg auuuuagaaa ggacuuccag uucuauaaag ugcgcgagau uaacaacuac     2940

caccaugcuc acgaugccua ccugaaugcu guugugggca ccgcccucau caagaaauau     3000

cccaagcugg aaucugaguu ugucuacggg gacuauaagg uguacgaugu ucggaaaaug     3060

aucgccaaga gcgagcagga aauuggcaaa gcuacagcga agagauucuu uuauuccaac     3120

aucaugaauu ucuuuaaaac cgagauuaca cuugccaacg gagaaauccg caagcggccu     3180

cugauugaga ccaaugaaga gacgggcgag aucgugugga acaaggggag agacuucgcu     3240

acaguccgca aagugcucag caugccccaa guuaauaucg ugaagaaaac cgaaguccag     3300

acaggagccc ugaccaacga gucuauuuac gcccggggcu ccuuugauaa gcugaucagc     3360

agaaaacauc gcuucgaauc cucuaaguau gggggauuug gcagcccuac cgugacauac     3420

uccgugcuug ucguugcuaa aucuaaggug caggacggga aggugaaaaa gauuaaaacg     3480

ggcaaggagc ugaucggaau uacccuccug gauaaacucg ucuucgagaa gaauccccug     3540

aaguuuaucg aagacaaagg cuacgggaac gugcagaucg acaagugcau uaaacuuccg     3600

aaguauagcc uguucgaguu cgaaaaugga acacggagaa ugcuggccuc cguuauggcg     3660

aacaauaacu cucggggcga ucuccaaaaa gcuaaugaga uguuucugcc ugccaaacuu     3720

gugacccugc ucuaccacgc ccauaagauc gaaagcucca aagagcugga gcacgaggcu     3780

uauauucucg accacuacaa cgaucuguau cagcuucuga gcuacaucga acgcuucgcc     3840

ucucuguaug ucgacgugga aaagaacauu uccaaaguua aggagcucuu uagcaauauc     3900

gaaucuuacu ccauuagcga gaucuguucu uccgugauca accugcuuac acugaccgcg     3960

agcggggcuc ccgccgauuu caaauuucuc ggaacgacaa uuccuagaaa gcgguacggc     4020

agcccccaga gcauccuguc uagcacccuc auucaucaau ccaucaccgg gcuguaugag     4080

acacgcauug accuuucuca gcugggcgga gauuga                               4116


<210>  4306
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4306
augaacaaua acaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucaucacag augacuauaa gguucccucu aaaaagauga gagugcucgg caauacggau      120

aaacgguuca uuaagaaaaa ccugaucggg gcccuucugu uugacgaggg aaccacagcu      180

gaagaucgca gacucaagcg gaccgcgcgc agacgguaca cacgcagaaa gaaucggcug      240

cgcuaucuuc aggagauuuu cgcugaagag augagcaaag uggacuccuc uuucuuccac      300

agacuggaug acagcuuucu cgucccugaa gauaaguccg gcucuaaaua cccgaucuuc      360

gccacccugg ccgaggagaa ggaauaucau aaaaaguuuc ccacaaucua ccaucugcgg      420

aagcaccucg cugacagcaa agagaaggcc gaucugcgcc uuauuuaucu ggcucucgcc      480

cacaucacca aauacagagg gcauuuccug uacgaagagg cguuugacau uaagaacaau      540

gauauccaaa agauuuucaa cgaguuuauc aauauuuaug acaacacguu cgaaggaucc      600

agccuuucug gccagaaugc ccagguggag gcuaucuuua ccggcaagau cuccaaaagc      660

guuaagcggg aacacgugcu gaaacucuuc ccugacgaga aaucuacagg gcuguucucc      720

gaauuucuga agcucauugu cggaaaccag gccgauuuca aaaagcacuu ugaccuggag      780

gagaaagcua gccuucaauu cucuaaggau accuacgacg aagaucugga gaaucuccug      840

ggccagaucg gggacgauuu ugccgaccuu uuccuggugg cgaagaaacu cuaugaugcc      900

auucugcugu ccggaauccu cacaguuacc gaccccagca cgaaggcucc ucuguccgcc      960

ucuaugauug aacgcuacga gaaccaucag gaagaucuug cugcccugaa acaguuuauc     1020

aagaacaauc uccccgagaa auaugccgag guguucagcg accaauccaa ggacggcuac     1080

gcuggguaua ucgauggaaa gacaacccag gaagcguuuu acaaauacau uaagaaccug     1140

cuuagcaaau ucgagggcgc cgacuauuuu cuggauaaga ucgaaagaga ggacuuccuc     1200

cggaaacaga gaacauuuga uaauggcucu auuccucacc agauccaucu gcaagaaaug     1260

aacgcuauuc ugcgccggca gggggagcac uacccgcucc ugaaggagaa uaaggagaaa     1320

aucgaaaaga uccuuaccuu ccgcauuccc uauuacgucg gaccucuggc cagaggcaac     1380

cgggacuucg ccuggcucac cagaaauucc gaucaggcua uccgccccug gaacuuugaa     1440

gagauugugg acaaagccag cucugcggag agcuucauca auaagaugac aaacuaugau     1500

cuguaccugc cugaagagaa agugcuuccc aagcacuccc uccuguauga aacguuugcu     1560

gucuacaaug agcugaccaa aguuaaguuc auugccgaag ggcuccggga cuaccaauuu     1620

uucgauucug gacagaagaa acagaucgug aaccagcugu uuaaggagaa aagaaaggug     1680

acagagaaag acauuaucca uuaucuucac aaugucgaug gcuacgacgg gaucgaacug     1740

aagggaauug agaagcaauu caacgccagc cucuccaccu aucaugaccu gcugaaaauc     1800

auuaaggaua aagaauuuau ggacaaucac aagaaccagg agauccuuga aaacauugug     1860

cauacacuca ccaucuucga ggaucgcgag augaucaaac agcggcuggc ucaguacgac     1920

agcauuuuug augaaaaggu uaucaaggcc cugacgagac gccacuauac aggcuggggc     1980

aaacucucug cuaagcugau uaaugggauc ugcgacaaaa agaccggaaa aaccauucuu     2040

gauuaccuga ucgacgaugg cuauaacaau cggaacuuca ugcaacucau caaugacgau     2100

gggcuguccu uuaaggagau uauccagaag gcccaggugg ucggaaaaac agacgaucug     2160

acccaggugg uuagagaacu uagcggcucu ccggcgauua agaaagggau ccuccaaucc     2220

auuaagaucg uggacgagcu ggucaaaauc augggcuacg ccccugaaag cauugugauc     2280

gagauggcuc gggagaacca gacaacggcc aagggaaaga aaaauuccca gcagcgcuac     2340

aagagaauug aagacgcucu gaaaaaccuc gcccccggcc uggauucuac caucagcaag     2400

gagaauccua cagacaacau ucaacuucag aaugaucggc uguuccucua uuaccugcag     2460

aacgggaaag acauguauac cggagaagcg cuggauauca aucaacuuuc cucuuacgac     2520

auugaucaca ucauccccca ggccuucauu aaggacgaua gccucgacaa cagaguccug     2580

acauccagca aagauaaucg cggcaagucu gacaacgugc ccucccugga gguugugaag     2640

aaacggaagg cuuuuuggca gcagcuccug gacagcaaac uuaucucuga gagaaaguuc     2700

aacaaucuga ccaaagccga acgcggggga cucgaugaga gagacaaggu cggcuuuauu     2760

aagcggcaac ugguggaaac gcgccagauc accaaacaug uugcucggau ucuggaugcc     2820

agauucaaca cagagguguc cgaaaagaau cagaaaaucc gcagcgucaa gaucauuacc     2880

cuuaaaucua accuggugag caauuuuaga aaggaguuca agcucuauaa agugcgggag     2940

aucaacgacu accaccaugc ccacgaugcu uaucugaaug cgguugucgc caaggcuauu     3000

cucaaaaagu acccuaaacu ggaaccggag uuuguguacg gggacuauca gaaguacgau     3060

cuuaagcgcu auaucuccaa aagcaaggac cccaaagaag uggagaaggc cacagaaaaa     3120

uacuucuuuu auucuaaccu gcucaauuuc uuuaaggagg agguccacua cgccgauggc     3180

accauuguga aaagagaaaa caucgaguau uccaaggaca cgggagaaau cgcuuggaau     3240

aaggagaaag auuucguuac aauuaagaaa gugcugagcu acccucaagu caacaucgug     3300

aagaaacggg aaguucagac cggcggguuc uccaaggagu cuauucugcc caagggaaau     3360

agcgacaaac uuaucccucg caagacaaaa gauauucugu gggacaccac caaguacggc     3420

ggguuugacu cccccgugau cgccuauucu auucuccuga ucgcggauau cgagaaagga     3480

aaggcuaaga aacucaagac agucaaaacg cuggugggca uuaccaucau ggaaaaggcc     3540

gccuucgaga aaaacccgau uacauuucuu gaaaauaagg gguaccauaa cguuagaaag     3600

gagaacaucc ugugucuccc uaaauauagc cuguucgagc uggaaaaugg ccggagacgc     3660

cuucuggcuu ccgccaagga gcuccagaaa ggaaacgaaa uugugcugcc cgucuaccuc     3720

accacacugc uuuaucacuc uaagaaugug cauaaacugg acgagccuga acaccuggag     3780

uacauccaga agcaucggua ugaguuuaaa gaucuccuga accuugugag cgaauuuucc     3840

caaaaguacg uccuggcgga ggcuaaucuc gaaaagauca aaagccugua cguugacaac     3900

gagcaggccg auauugaaau ccucgccaau ucuuuuauua accugcuuac cuucacggcu     3960

cugggcgccc ccgcugccuu uaaguucuuu gggaaagacg uggauagaaa gcgcuauaca     4020

accguguccg agauccugaa ugcgacccuc auucaccaga gcaucacagg acuguacgag     4080

acccggaucg accuuucuaa acugggcgaa gauuga                               4116


<210>  4307
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4307
augaccgaga agaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucaucacgg augacuauaa aguucccgcu aagaaaauga aggugcucgg caacaccgau      120

aaaaaguaca uuaagaaaaa ucugcuuggg gcccuccugu ucgacaacgg agaaacagcg      180

gaggcuacca gacugaagcg gacagcccgc agacgguaua cccgcagaaa gaaucggcuu      240

cgcuaccugc aggaaaucuu ugccgaggaa auggcuaagg uggaugaguc uuucuuuuau      300

agacucgacg agagcuuccu gacaaccgau gacaaagauu uugaacggca cccuauuuuc      360

ggcaacaagg ccgacgagau caaguaccau caagaauuuc cgacgaucua ucaucugcgc      420

aaacaccucg cugauuccca cgagaaggcc gaccugagac uuauuuaccu ggcgcucgcc      480

cauaugauca aauuccgggg gcacuuucug auugagggag aacuuaaugc ugagaacacc      540

gauguccaga agcuguucga agccuuugug gagguuuacg accgcacauu cgacgauucu      600

aaucucagcg aaaucaccgu ggacgcuucc ucuauucuga cagagaaauu uagcaagucc      660

agacggcugg agaaccucau caaacacuau cccaccgaaa agaagaauac gcuguucggc      720

aaccuugucg cccuggcgcu cggccugcag ccuaauuuca aaaccagcuu uaaacuuucu      780

gaggaugcca aacugcaguu cuccaaggac acauacgaag aggaucucga ggaacugauu      840

gggaaaaucg gagacgagua ugcugaucug uuuacaagcg ccaagaaccu cuacgacgcu      900

auccugcuuu cuggcauucu gaccguggcc gauaauacca caaaggcucc ccucuccgcc      960

agcaugauca aacgcuauaa cgaacaucaa guugaccuga agaaacuuaa ggaguucauu     1020

aaaaacaaug cgucugauaa guacgacgaa aucuuuaacg acaaggauaa aaauggguau     1080

gccggauaca uugagaacgg cgugaagcag gacgaguucu acaaauaucu gaagacgacc     1140

cucuccaaaa ucgaugggag cgacuacuuu cuggauaaga ucgaaagaga ggacuuccug     1200

cggaagcaga gaacauuuga uaauggaucc auuccucacc agauccaucu ccaagaaaug     1260

cacgcuauuc ugcgccggca gggcgagcac uaugccuucc uuaaagaaaa ccaggccaag     1320

aucgagaaaa uucugaccuu uagaaucccc uacuaugucg gcccucucgc ucgcaaaaau     1380

ucucgguucg ccugggcgga guaccauagc gacgaaaaga ucacaccgug gaacuuugau     1440

gagauuaucg acaaggaaaa auccgcugag aaguucauua ccagaaugac gcugaaugau     1500

cuguaucuuc ccgaagagaa ggugcucccu aaacacagcc ugguguacga gacauacacc     1560

gucuauaacg aacugaccaa gguuaaauac gugaaugagc aggggaaguc uaacuucuuu     1620

gacgccaaua ugaaacaaga aaucuucgag cauguguuua aggaaaaccg caaggucaca     1680

aaagauaagu uccucaauua ucugaacaaa gaguuucccg aguaccggau ucaggaccuu     1740

aucggacugg acaaggaaaa caaauccuuc aaugccagcc ucggcaccua ucacgaucug     1800

aagaagauuc uggacaaauc uuuucuugau gacaagacaa acgagacgau caucgaagau     1860

auuauccaga cccucacacu guucgaggac agagauauga uucgccaacg gcugcagaaa     1920

uacagcgaca ucuuuacccc ucagcagcuc aagaaacugg aaagacgcca uuauacaggg     1980

uggggacggc uuuccuacaa gcugauuaau ggcaucagaa acaaggagaa ugggaaaucu     2040

auccucgauu accugauuga cgauggauau gcuaaccgga auuucaugca acugaucagc     2100

gacgauaccc uucccuuuaa gcagauuauc aaagacgccc agauuaucgg cgacaucgau     2160

gacgugacgu ccguugugcg cgagcucccg ggcagcccug cuauuaagaa agggauccug     2220

cagucuguca agauugugga ugaacugguu aaagugaugg gacacaaccc cgacaauauc     2280

gucauugaga uggccagaga aaaccaaacc acaaaucggg gcagaaacca gucccagcag     2340

cgccucaaga agcugcaaga uagccuuaaa gagcuggggu cuaauauccu caacgaggaa     2400

aagccuuccu acaucgaggg aaaaguggaa aauaaccauc ugcaggacga ucggcuguuc     2460

cuuuauuaca uucagaacgg caaggacaug uauaccgggg augagcucga caucgaucac     2520

cugagcgacu acgacauuga ucacaucauu ccccaggcgu uuaucaaaga cgauuccauu     2580

gacaauagag uccugacauc uagcgccaag aaccgcggca aguccgauga cgugcccucu     2640

cucgauaucg uucaugacag aaaagcugau uggauccggc uguauaagag cggacuuauu     2700

uccaaacgca aguucgacaa ucugaccaaa gccgaaagag gcgggcucac ggagaacgau     2760

aaggcuggau ucaucaagcg gcaacuggug gagacacgcc agauuaccaa acacgucgcc     2820

cagauccugg acagcagauu uaauacagaa cgggaugaga acgacaaggu gauucgcaau     2880

guuaaaguga ucacccuuaa gucuaaccug gucucccaau ucagaaaaga cuuuaaguuc     2940

uacaaggugc gggaaaucaa ugauuaccac caugcgcacg acgccuaucu caacgcugug     3000

guuggcaccg cccugcucaa aaaguacccu aaacuggcuc cggaguuugu cuauggggaa     3060

uacaagaaau augauauucg caaguucauc acaagcucug gagacaaggc cacggccaaa     3120

uacuuuuucu auuccaaucu uaugaacuuu uucaagagag ugauucggua cagcaauggc     3180

aaaguggucg ugagacccgu uaucgagugc ucuaaggaua ccggggagau ugcuuggaac     3240

aaacagacag acuuugaaaa ggugcgccgg guccugagcu acccucaggu gaauaucguu     3300

aaaaaggugg agacccagac aggcggauuc uccaaggaaa gcauccuccc caaaggcaac     3360

ucugauaagc ugauuccuag aaaaaccaag aaauuccgcu gggacacgcc caaguauggg     3420

ggauuugauu ccccgaacau cgcguacagc gucuucguga uugccgacgu ugagaagggc     3480

aaagcuaaga aacugaagac agugaaagaa cuugucggga ucuccauuau ggagcggucu     3540

agcuuugagg aaaauccugu gguguuccug gagaagaagg gauaucaaaa cguccaggaa     3600

gauaaucuca ucaaacugcc caaguacucc cucuuugagu ucgagggcgg gagacgccgg     3660

cugcuugccu cugccagcga acugcagaaa ggcaacgagg uugugcucuc cagacaucug     3720

guggaacugc uuuaucacgc ucaucgcguc aauucuuuua acaauagcga gcaccugaag     3780

uacguguccg aacacaaaaa ggaguucgga gagguucuca gcugugugga aaacuuugcc     3840

aagucuuaug ucgacgugga gaaaaaucug ggcaagauuc gggcgguugc ugauaaaauc     3900

gacaccuucu ccaucgaaga cauuagcauc ucuuuuguga accuccugac ccuuacagcc     3960

cugggggccc cugcugauuu caauuuucug ggagagaaga uucccagaaa acgguacacc     4020

uccacaaagg aaugccucaa cgccacgcug auccaucaga gcauuaccgg ccuuuacgag     4080

acacgcaucg accugucuaa acucggggag gaauga                               4116


<210>  4308
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4308
augaaccuga gaaaugugga gagcuaccag gucggccucg acaucgggac cggauccguu       60

ggcugggccg ugcuggauga caacggggau cuuugccggu ucaagggaaa acccacaugg      120

ggcucucgcg uguuuccuac ggcugaaacc gccgcggagg cuagagucca ccgggggcaa      180

cgcagacggu augaccgcag acggcagcgc auugaucugc uccagagauu cuuugccgac      240

gaaguggcca agguugaucc ggaguucuuu auccggcuga aucaggcucg ccuucauccc      300

gaaaacagag acccugccca cgcugauuac cgguggcccc uguucaaugg aagcaacuuu      360

acagagaaag acuauuaccg ccaauucccu accauuuauc aucucagagc ccaucugcug      420

aaguccgaug agcccgcgga cauccggcuc guguaccugg cccuucacaa uauugucaaa      480

cacagaggca acuuucugca ccaggauaau ccuucucuca gcgcucgcaa cgccaaugug      540

ggcgacgcug uugaagcccu gugucuugcg cuggacgagu ggugcggggc ccaugaugug      600

gaaugugcuu gcgacgaggc cgcucucgcc gaggcccugg gagaugcuuc ccugcggccg      660

ggcgaaaaga gagagcgcgc ggaagccgcu cucggggucg agaaggccca caaaaagcug      720

gccaaagcug ugucuagcgc cguggucgga uaugcggcug aauuuggcca uguuuucuuc      780

gccgaguccg aggggagcaa guuugcccuu ucuaacgacg aaaaagugga ggcuuacgug      840

ugucccgaug aaggacggga ccuguucgau gccaugagag cuguccacuc cagcuacgug      900

cucaugggca uccugucuga ggcccagggc gaacuucugu ccguuugcaa ggugggggag      960

uaugagcgcu acaagcggga ccucgaaaca cugaaaagcc uggucagagc guauggaccu     1020

gagcgcuacg augaguucuu uuguggcccc uucuaugaag ggacccggga cuacgauccu     1080

ggaaaggccc agggcuauac aaaauacaau cucggggcuu cuaagcuguc ccacgaagac     1140

uuucuuaaag aggugcaaaa gcugcucaaa gacaucggag cccaggcuga ugagagauac     1200

gccgacauug aacgcgcgau cgaggccggc gcuuuccugc ggagacuuaa gaccagcgau     1260

aacggcacga uucccuauca gcugcaucuc gaagagaugg acgccaucau ugagcggcag     1320

gcugccuuuu acccguuccu ggcccaagaa aaggauaaac ugaagucccu cguuucuuuu     1380

cgcaucccuu auuacguggg gccccugacc cagaaaaaug cugcgagaga cggaaagggc     1440

gaggcccggu ucgcuuggag cuccagaaaa ccugggaugg aagaugcccg cgucuauccc     1500

uggaacuggg aggacgugau ugauaaggac cagagcgccg aagauuuuau ccggagaaug     1560

acaggaaccu gcacauaccu uaagggcgag cccguccugg cucgcuguuc ucucgacuau     1620

gagcuguucu gcgugcugaa cgaacuugcc ggggcgagau ggacccaaga uggcgacaaa     1680

uccuaccggu uugacgcugc cgaucgccuc ggaaucguug aggaccuguu caagaaaaga     1740

aagcggguga cguacgaagc cgcugagaaa uggcuggccc gcacacacgg cccugaaagc     1800

gggaagucug cgggaggcgc uuaucauguc agcgggggac agggcgagga ugccuuugag     1860

ucccagcucu cuagccacua cgacuucugu aagauucugg ggguggauga acuuuccgac     1920

gccgaugagg cuauggccga agacaucauu cuguggaaua ccguuuucga ggauagaagc     1980

auccucaaac ggaagauuaa acuggcuuau ggcgaccgcc ugggagaugc ccaggugaga     2040

aaacuuugcc aaaaacggua cacaggcugg gggcgccucu cuggagaacu gcugagaggc     2100

cuccgguccg cgaccgacaa cgggccgaag accaucaugg auauccugcu ugagggagac     2160

cccaaucaug ccagacgcau gggcgcugcc augaaccuga uggagauucu ccacgacgau     2220

cugcuggaau uucaggcuaa gaucgacgag cacaaucggg aaagagcccu ugccgucggg     2280

ggcaugagcg uggaugaggu gacaggaucu ccugcucucc gccggggcau uaaccaggcg     2340

cugagaguug ucgacgagau cgccgggauu gcuggaaaac ccccugccca uaucuaugug     2400

gaagugacgc gcgaugaggg cgcccggaau aaggggagac gcaccacauc ccgguacaga     2460

gcucuggaag ccgcgcucag cuccaucaaa gcucagugcg ccgacgucua ugccgagcug     2520

aaggaacggg auccccgcgc ucuugacgau gagagacuga cccucuacuu caugcaaugu     2580

ggaaaaugca uguacucugg cgacgcccug gauauuaaca agcugcagac auaucaggug     2640

gaccacaucc uuccgcagag cuacguuaaa gacgauuccu uugagaauaa ggugcucguc     2700

cggccugaca ccaaccaacg caagggggau acgcugcugc ucgacgaaag acugauugcg     2760

cggaugagag gcuucuggcg cacacuucgg gaugcugagc ugaucggaga aaagaaguuu     2820

aauaaccuca ccagaaccuc ugugagcgac gagcagauga aaaaguucgu uucccgccag     2880

cugguggaaa caggccagau ggucaaacaa gugcagcagc ugcuucaagc ccgguauccc     2940

gagagcgagg uucagccugu gaaggccggg gucucucauc agcugagagu gggaugugcu     3000

cucgccaagu cccgcgaaau uaaugauuuu caccacgcuc augacgccua ccuggcgugc     3060

caggugggcc gguucgucca auauagacac agcgccguuu acucugagcc cgugaaaaug     3120

gcugccgugg ucaagcaguu uaucaaaaag caggcugaag auuaucgcag aacccgggag     3180

cucccugggu ccgacggauu cauugugagc ucuuuuauga cacccggcuu cgauguuaaa     3240

acgggcgaag uguuucgcga cgccugggau gccggggcug agaucgacag aauccggcgc     3300

uguuucgacu acaaggauug cuucauuagc agaaugccgg aggaaaccca uggagcguuu     3360

ugggacgcca caaucuacuc cccucgggcu gccggcaaga cccugagccu uccccugaaa     3420

agagggcucg auccccgcaa guauggaucu uacucccggg agcaguucgc cuacuuuuuc     3480

gucuaugaag cugugaaacc uaagaaaaag caaagaguuc uggaguuugc cccggugccc     3540

guccgcgugg cgagcgcucu ggccaacgac ccugaugccc uugaggacua ugcucgggaa     3600

cuggccgagg cggaaggccu cguuuucgag cggauuagac gcagaaaggu guacaaauau     3660

cagcagauca uugucgggga uucccggcug uacgugacag gacgcgaaga ggugagaaau     3720

gcccggcagc ucgcuuuuuc ucaagacgag accaagcuga ucugucgcau cgaacagggc     3780

gaggaaugcg aggaaggcga gcuucuggau cucuucagaa gcaugcagcg gaaauaccug     3840

gccugugcuc cccgccuggc cagacuuaug aaggucgcug agauggaaga gggguuuauu     3900

gccgcgacgg ccuccggaaa acgggaaguu cugcucucuc ugaccagcau cgcugccgcu     3960

cacagaaacu ccauugaccu caagccuauc ggccgcaaag ggucuagcgg acaaaugaag     4020

auuacauucu ccaaggagcu gagcaccaac ggcauccuuu uuguggauuc uuccgugaca     4080

gggauguucg aacggagaug ccgccuggga cuguga                               4116


<210>  4309
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4309
auggagaaca agaauuacag caucggccug gacauuggga ccaacuccgu gggauggucu       60

gucaucacag augacuauaa aguucccagc aagaaaauga aggugcucgg caauacggau      120

aaaagauuca uuaagaagaa ccugaucggg gcccuucugu uugacgaagg aaccacagcu      180

gaggcccggc gccucaaaag aaccgcgcgg cgcagauaca cacggcgcaa gaauagacug      240

cgguaucuuc aggaaauuuu cgcugaggaa auguccaaag uggauucuag cuuuuuccac      300

cgccuggacg auuccuuucu caucccugag gacaagagag gcucuaaaua cccgaucuuc      360

gccacccugg ccgaggaaaa ggaguaucau aagcaauuuc ccacaauuua ccaucugcgg      420

aaacagcucg cugauagcaa ggaaaagacc gaccugcgcc uuaucuaucu ggcccucgcu      480

cacaugauua aguacagagg gcacuuccug uacgaggaua cguuugacau caaaaacaau      540

gauauucaga agaucuucuc cgaguuuauu agcaucuaug acaacaccuu cgaaggaucu      600

ucccuuagcg gccagaaugc ccaagucgag gcgaucuuua cagacaaaau uucuaagucc      660

gccaagcggg aacgcgugcu gaaacucuuc ccugaugaaa agagcaccgg ccuguucucu      720

gaauuucuga aacucaucgu ugggaaccag gcugacuuca agaaacauuu ugaucuggga      780

gagaaggcuc cccuucaguu cuccaaggac acauacgaug aggaccugga aaaucuccug      840

ggccagauug gggaugacuu ugccgaucuu uuccuggugg ccaaaaagcu cuaugacgcg      900

auccugcuga gcgggauucu caccgucacg gauccuucca caaaagcucc ccugucugcc      960

agcaugaucg agagauacga aaaccaccaa aaggaccuug ccgugcugaa acaguuuauc     1020

aagaauaacc ucccugagaa guaugacgaa guuuucuccg aucagagcaa agacggcuac     1080

gcuggguaua uugauggaaa gaccacacag gaggccuuuu acaaauacau caagaaucug     1140

cuuucuaaau ucgagggcac cgacuauuuu cuggaaaaga uugagcggga agauuuccuc     1200

agaaagcaac gcaccuuuga caacggcucc aucccgcacc agauucaucu gcaggagaug     1260

aacgccaucc ugcggagaca aggggaacac uaccccuucc ucaaagauaa uaaggagaaa     1320

aucgagaaga uucugacauu ccgcaucccu uauuacgugg gaccccuugc ucggggcaac     1380

agagacuuug cguggcugac gcgcaauagc gaugaagcca uucggccuug gaacuucgag     1440

gaaaucgucg acaaggcuuc uagcgccgag gauuuuauua auaagaugac caacuaugac     1500

cucuaccugc ccgaggaaaa agugcugccg aagcauuccc uucucuauga gacauucgcc     1560

guguacaaug aacugaccaa ggucaaauuu aucgcugagg ggcugagaga uuaccaguuc     1620

cucgacucug gacagaagaa acagauuguu acacaacugu uuaaggaaaa acgcaaggug     1680

accgagaagg acaucaucca guuccuucac aacguggaug gcuaugacgg gauugagcug     1740

aaaggaaucg aaaagcaguu uaaugccagc cucuccacgu accacgaucu gcugaaaauu     1800

aucaaggaca aagaguucau ggaugacagc aagaacgaag agauucuuga aaauaucguc     1860

cauacacuca ccaucuuuga ggaucgggag augauuaagc agagacuggc gcaauaugac     1920

ucucuguucg augaaaaagu gaucaaggcu cucauucgcc ggcacuacac cggcuggggc     1980

aaacuguccg ccaaacuuau cgacgggauu ugcgauaaac agacaggaaa caccauccug     2040

gacuaucuca ucgaugacgg caagaauaac agaaauuuua ugcagcugau uaacgacgau     2100

gggcugagcu ucaaggagau cacacagaaa gcccaaguug ugggaaagac ggacgauguc     2160

aaacaggugg uucaggaacu uccuggcucu cccgcuauua agaaagggau ccuccagucc     2220

auuaagaucg uggacgagcu ggucaaagug augggccaua ccccugaaag caucgucauu     2280

gagauggccc gggagaacca aacaaccgcu cgcggaaaga aaaauuccca gcagagauac     2340

aaacggaucg aagaugcccu gaagaaccuc gcgucuggcc uggacagcaa uauucuuaaa     2400

gagcacccca caaacaauau ccaacugcag aacgauagac ucuuccugua uuaccugcag     2460

aaugggcgcg acauguacac cggaaagccc cuugauauua accagcucuc cucuuaugac     2520

aucgaucaua uugugccuca agccuuuauc aaagacgaca gccuggauaa ucggguucug     2580

acgucccuca aggacaacag aggcaagagc gauaaugugc cgucuaucga agucgugcag     2640

aaacgcaagg cuuucuggca gcagcugcuu gacuccaaac ugauuagcga gagaaaguuu     2700

aacaaucuca ccaaagccga gcggggagga cuggaugaac gcgacaaggu uggcuucauc     2760

aagagacaac ugguggagac acggcagauu accaaacacg ucgcucagau ccuugaugcc     2820

cgcuuuaaca aggaagugaa ugagaaagac aagaaaaaca gaacagugaa gauuaucacc     2880

cugaagucua accucguuuc caauuuccgg aaagaauuuc gccuguacaa ggucagagag     2940

aucaacgauu aucaccaugc gcacgacgcc uaccucaaug cugugguggc caaagcuauu     3000

cugaagaaau aucccaaacu ugagccugag uucgucuacg gggauuauca gaaguacgac     3060

cugaaacggu acaucagccg cucuagaaac cccaaggagg uggaaaaagc cacggagaag     3120

uacuuuuucu auagcaaucu ccugaacuuu uucaaagaag agguucauua ugccgauggc     3180

acaauuguga agcgggagaa uaucgaauac uccaaagaca ccggagagau ugcuuggaac     3240

aaggaaaagg acuucgcgac aaucaaaaag guccugagcu auccucaagu gaauaucguu     3300

aaaaagagag aggugcagac cggcggguuu ucuaaagaau ccauucuucc caagggaaac     3360

agcgauaagc ugaucccgcg caaaaccaag gacauucucu gggagacaac gaaauacggc     3420

ggguucgauu ccccugucau cgccuauucu auucugcuca ucgcugacau ugagaaggga     3480

aaagccaaga agcugaaaac cgugaagaca cuuguuggca ucaccaucau ggaaaaagcc     3540

gcuuuugagg aaaaucccau uacauuccug gagaacaagg gguaccacaa ugugcggaaa     3600

gagaacaucc ucugucugcc uaaguacagc cuguuugaac uugagaaugg cagacgccgg     3660

cugcucgccu ccgcgaagga acugcagaaa ggaaacgaga uuguccuccc cguguaucug     3720

accacgcuuc uguaccacuc uaagaacgug cauaaacugg augaaccuga gcaccucgag     3780

uauauccaaa agcauagaaa ugaguucaaa gaccugcuua accuggucag cgaauuuucc     3840

cagaaguacg uucucgcuga ugccaaucug gaaaagauuc agaaccucua ugccgacaau     3900

gagcaggcug auaucgaaau ccuggccaac ucuuucauua aucuucugac auuuaccgcg     3960

cugggcgcuc ccgccgccuu caaguuuuuc gggaaggaca ucgaucgcaa acgguacacc     4020

acagugagcg agauucucaa cgcuacccug auccaccaau ccauuacagg acuuuaugag     4080

acgagaaucg accugagcaa gcucggcgaa gauuga                               4116


<210>  4310
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4310
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug auuccggaga gaccgccgaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuaga      240

uaucugcagg agaucuucuc uagcgaaaug uccaaagugg acgauagcuu uuuccaucgg      300

cuugaggaau cuuuucuggu cgaggaggac aagaaacacg aacgccaucc uaucuucggc      360

aacauugugg augagguugc cuaccacgaa aaguauccga ccaucuacca ucucagaaag      420

aaacuggccg acuccacaga uaaggcugac cugcggcuca uuuaucuggc ccuugcucac      480

augaucaaau uucgcgggca cuuccugauu gagggagauc ucaaucccga caacagcgac      540

auggauaagc uguuuaucca acuugugcag accuacaauc agcuguucga ggaaaacccu      600

auuaaugccu cuagagucga cgcgaaagcc auccucuccg cucggcugag caagucucgc      660

agacuggaga accucaucgc ccagcugccc ggcgaaaaac ggaauggccu uuuugggaac      720

cugauugcuc ucucccuggg acuuacgccu aauuucaaga gcaacuuuga ucuggccgag      780

gacgcgaagc uccaacuguc uaaagauacc uacgacgaug accuggauaa ucuccuggcc      840

cagaucggcg accaguaugc ugaucuuuuc cuggccgcua agaaccucuc cgacgccauu      900

cugcuuagcg acauccugag agugaacucc gaaauuacaa aagcuccccu cucugcuagc      960

augaucaagc gcuacgauga gcaucaccag gaccugaccc ugcucaaagc gcugguucgg     1020

caacagcuuc cugagaagua uaaggaaauc uucuuugauc aguccaaaaa uggguacgcc     1080

ggauauauug acggcggggc uagccaggag gaguucuaca aguuuaucaa accgauucug     1140

gaaaagaugg auggaacaga ggaacuccug gccaaacuga acagagagga ccuucuccgc     1200

aagcaacgga ccuucgauaa uggcucuauc ccccaucaga uucaccuggg cgaacugcac     1260

gccauccuca gacgccagga ggacuuuuau ccuuuccuga aggauaaccg ggaaaagauc     1320

gagaagauuc uuacguuuag aauccccuac uacguggggc cucuggcucg cggaaauucc     1380

cgguucgccu ggaugacaag aaaaagcgaa gagaccauua cacccuggaa cuuugaggaa     1440

gucguggaca agggcgcguc ugcucagagc uucaucgagc gcaugaccaa uuuugauaaa     1500

aaccucccga augaaaaggu gcugccuaag cauucccugc uuuaugagua cuucaccguc     1560

uauaacgagc ucacaaaagu uaaguacgug acggaaggga ugcggaaacc cgaguuucug     1620

ucuggaaagc aaaaagaagc cauuguggac cuccuguuca agaccaauag aaaagucaca     1680

gugaagcagc ugaaggagga uuauuucaaa aagaucgaau gcuuugacag cguugagauu     1740

uccggcgugg aggaccgguu caacgccagc cuugggaccu accacgaucu gcucaaaauc     1800

aucaaggaca aagauuuucu ggacaaugaa gagaacgaag auauucugga ggacaucguc     1860

cuuacacuca cccuguucga agauaaggag augauugagg aacgccugaa gaaauaugcu     1920

aaucucuuug acgauaaggu gaugaaacag cugaagagac ggcauuacac gggauggggc     1980

agacuuucuc gcaaacugau caacggcauu cgggacaagc aauccgggaa gacaauccuc     2040

gauuuccuga aaagcgacgg auuugccaac agaaauuuca ugcagcugau caacgaugac     2100

ucucuuaccu uuaaggaggc uauucagaaa gcccagguuu ccggccaagg gcacagccuc     2160

caugaacaga ucgcgaaucu ggccggaucc ccugcuauua agaaaggcau ccugcagucu     2220

gugaaggucg uggacgagcu cgucaaggug auggggcaca aacccgaaaa cauuguuauc     2280

gagauggccc gcgagaauca gaccacacaa aagggccaga aaaacagcag agaacggaug     2340

aagcgcaucg aggaaggaau uaaagagcug ggcucccaga uccuuaagga gcaccccgug     2400

gaaaauaccc agcugcaaaa cgagaaacuc uaccuguauu accugcagaa ugggagagau     2460

auguaugucg accaggaacu ugauauuaac cggcugucug acuacgaugu ggaccauauc     2520

guuccucaga gcuucauuaa ggaugacucc aucgauaaua agauccucac acgcagcgac     2580

aaaaacagag gaaagucuga uaaugugccg uccgaggaag ucgugaaaaa gaugaaaaac     2640

uauuggcggc aacugcucaa ugcuaagcug auuacgcagc gcaaguuuga caaccuuacc     2700

aaagccgaga gaggcgggcu gagcgagcuc gacaaggcgg gauucaucaa acggcagcug     2760

guggaaacac gccaaauuac caagcacguu gcccagaucc uggauucuag aaugaacaca     2820

aaauacgacg agaaugauaa acuuauucgg gaagucaagg ugaucacccu gaaauccaag     2880

cucgugagcg acuuuagaaa agauuuccag uucuauaagg uccgcgagau uaacaauuac     2940

caucacgcuc acgacgccua ccugaacgcu gugguuggca cggcccucau caaaaaguau     3000

accaagcugg aaucugaguu uguguacggg gauuauaaag ucuacgacgu gcggaagaug     3060

aucgccaaaa gcgagcagga aauuggcaag gcuacagcga aaagauucuu uuauuccaau     3120

aucaugaacu ucuuuaagac cgagauuaca cuugccaaug gagaaauccg caagcggccc     3180

cugauugaga ccaacgaaga gacgggcgag aucguuuggg auaaagggag agacuucgcu     3240

acagugcgca agguccucag caugccucaa gugaauaucg uuaaaaagac cgaagugcag     3300

acaggagccc ugaccaacga gucuauuuac gcccggggcu ccuuugauaa acugaucagc     3360

agaaagcauc gcuucgaauc cucuaaauau gggggauuug gcagccccac cgucacauac     3420

uccgugcuug uggucgcuaa gucuaagguu caggacggga aagugaagaa aauuaagacg     3480

ggcaaagagc ugaucggaau gacccuccug gauaagcucg uguucgagaa gaauccucug     3540

aaauuuauug aagacaaggg cuacgggaac guccagaucg acaaaugcau caaacuuccc     3600

aaauauagcc uguucgaguu cgaaaaugga acacggagaa ugcuggccuc cgugauggcg     3660

aacaauaacu cucggggcga ucuccaaaag gcuaacgaga uguuucugcc ggccaaacuu     3720

guuacccugc ucuaccacgc ccauaagauu gaaagcucca aggagcugga gcacgaggcu     3780

uauauccucg accauuacaa ugaucuguau cagcuucuga gcuacauuga acgcuucgcc     3840

ucucuguaug uggacgucga aaagaacauc uccaagguga aagagcucuu uagcaauauu     3900

gaaucuuacu ccaucagcga gauuuguucu uccguuauca accugcuuac acugaccgcg     3960

agcggggcuc cugccgauuu caaguuucuc ggaacgacaa uccccagaaa gcgguacggc     4020

uccccucaga gcauucuguc uagcacccuc auccaccaau ccauuaccgg gcuguaugag     4080

acacgcaucg accuuucuca gcugggcgga gauuga                               4116


<210>  4311
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4311
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcc cuucuguuug auuccggaga gaccgccgaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuaga      240

uaucugcagg agaucuucuc uagcgaaaug uccaaagugg acgauagcuu uuuccaucgg      300

cuugaggaau cuuuucuggu cgaggaggac aagaaacacg aacgccaucc uaucuucggc      360

aacauugugg augagguugc cuaccacgaa aaguauccga ccaucuacca ucucagaaag      420

aaacuggccg acuccacaga uaaggcugac cugcggcuca uuuaucuggc ccuugcucac      480

augaucaaau uucgcgggca cuuccugauu gagggagauc ucaaucccga caacagcgac      540

guggauaagc uguuuaucca acuuguccag accuacaauc agcuguucga ggaaaacccu      600

auuaaugccu cuagagugga cgcgaaagcc auccucuccg cucggcugag caagucucgc      660

agacuggaga accucaucgc ccagcugccc ggcgaaaaac ggaauggccu uuuugggaac      720

cugauugcuc ucucccuggg acuuacgccu aauuucaaga gcaacuuuga ucuggccgag      780

gacgcgaagc uccaacuguc uaaagauacc uacgacgaug accuggauaa ucuccuggcc      840

cagaucggcg accaguaugc ugaucuuuuc cuggccgcua agaaccucuc cgacgccauu      900

cugcuuagcg acauccugag aguuaacucc gaaauuacaa aagcuccccu cucugcuagc      960

augaucaagc gcuacgauga gcaucaccag gaccugaccc ugcucaaagc gcuggugcgg     1020

caacagcuuc cugagaagua uaaggaaauc uucuuugauc aguccaaaaa uggguacgcc     1080

ggauauauug acggcggggc uagccaggag gaguucuaca aguuuaucaa accgauucug     1140

gaaaagaugg auggaacaga ggaacuccug gccaaacuga acagagagga ccuucuccgc     1200

aagcaacgga ccuucgauaa uggcucuauc ccccaucaga uucaccuggg cgaacugcac     1260

gccauccuca gacgccagga ggacuuuuau ccuuuccuga aggauaaccg ggaaaagauc     1320

gagaagauuc uuacguuuag aauccccuac uacgucgggc cucuggcucg cggaaauucc     1380

cgguucgccu ggaugacaag aaaaagcgaa gagaccauua cacccuggaa cuuugaggaa     1440

gugguggaca agggcgcguc ugcucaaucc uucaucgagc gcaugaccaa uuuugauaaa     1500

aaccucccga augaaaaggu ccugccuaag cauucccugc uuuaugagua cuucaccguu     1560

uauaacgagc ucacaaaagu gaaguacgug acggaaggga ugcggaaacc cgccuuucug     1620

ucuggagagc agaagaaagc cauugucgac cuccuguuca agaccaauag aaaagugaca     1680

guuaagcagc ugaaggaaga uuauuucaaa aagaucgagu gcuuugacag cguggaaauu     1740

uccggcgucg aggaccgguu caacgcuagc cuugggaccu accacgaucu gcucaaaauc     1800

aucaaggaca aagauuuucu ggacaaugag gaaaacgagg auauucugga agacaucgug     1860

cuuacacuca cccuguucga ggauaaggaa augauugagg agcgccugaa gacguaugcc     1920

caucucuuug acgauaaagu uaugaagcag cugaaaagac ggcacuacac aggauggggc     1980

agacuuucuc gcaagcugau caauggcauu cgggacaaac aauccgggaa gaccauccuc     2040

gauuuccuua agagcgacgg auuugcuaac agaaacuuca uccagcugau ucaugaugac     2100

ucucugaccu uuaaagaagc cauccagaag gcccaggugu ccggccaagg gcacagccuc     2160

cacgagcaga uugcgaaucu ggcuggaucc ccugccauca aaaagggcau ucugcagucu     2220

gucaaagugg ucgacgaacu cgugaagguu auggggcaua agcccgagaa caucgugauc     2280

gaaauggcuc gcgagaauca gacaacccaa aaaggccaga agaacagcag agagcggaug     2340

aaacgcauug aagagggaau caaggaacug ggcucccaga uucuuaaaga gcaccccguc     2400

gagaauacac agcugcaaaa cgaaaagcuc uaccuguauu accugcagaa ugggagagau     2460

auguaugugg accaggagcu ugauaucaac cggcugucug acuacgaugu ugaccauauu     2520

gugccucaga gcuucaucaa agaugacucc aucgauaaua agguccucac gcgcagcgac     2580

aagaacagag gaaaaucuga uaaugugccg uccgaagagg ugguuaagaa aaugaagaac     2640

uauuggcggc aacugcucaa ugccaaacug auuacccagc gcaaguuuga caaccuuaca     2700

aaggcggaaa gaggcgggcu gagcgagcuc gacaaagccg gauucaucaa gcggcagcug     2760

gucgagaccc gccaaauuac aaaacacgug gcucagaucc uggauucuag aaugaauacc     2820

aaguacgacg aaaacgauaa acuuauucgg gaggugaagg ucaucacgcu gaaguccaaa     2880

cucgugagcg acuuuagaaa ggauuuccag uucuauaaag uucgcgaaau uaacaauuac     2940

caccaugccc acgacgcuua ccugaacgcc guggucggca ccgcccucau caagaaauau     3000

cccaagcugg agucugaauu uguguacggg gauuauaagg uuuacgacgu gcggaaaaug     3060

aucgcuaaga gcgagcagga gauuggcaaa gcgacagcca agagauucuu uuauuccaau     3120

aucaugaacu ucuuuaaaac cgaaauuaca cuugcuaaug gagagauccg caagcggccu     3180

cugauugaaa ccaacgagga aacgggcgag aucgucuggg auaaggggag agacuucgcc     3240

acagugcgca aaguucucag caugccccaa gugaauaucg ucaagaaaac cgaggugcag     3300

acaggagccc ugaccaacga aucuauuuac gcucggggcu ccuuugauaa gcugaucagc     3360

agaaaacauc gcuucgaguc cucuaaguau gggggauuug gcagcccuac cgugacauac     3420

uccguccuug uuguggccaa aucuaaggug caggacggga aggucaaaaa gauuaaaacg     3480

ggcaaggaac ugaucggaau uacccuccug gauaaacucg uguucgagaa gaauccccug     3540

aaguuuaucg aggacaaagg cuacgggaac guucagaucg acaagugcau uaaacuuccg     3600

aaguauagcc uguucgaguu cgaaaaugga acacggagaa ugcuggcguc cgugauggcu     3660

aacaauaacu cucggggcga ucuccaaaaa gccaaugaaa uguuucugcc ugccaaacuu     3720

gucacccugc ucuaccacgc ucauaagauc gagagcucca aagaacugga gcacgaggcc     3780

uauauucucg accacuacaa cgaucuguau cagcuucuga gcuacaucga acgcuucgcg     3840

ucucuguaug uggacguuga gaagaacauu uccaaaguga aggaacucuu uagcaauauc     3900

gagucuuacu ccauuagcga aaucuguucu uccgucauca accugcuuac acugaccgcu     3960

agcggggccc ccgccgauuu caaauuucuc ggaacgacaa uuccuagaaa gcgguacggc     4020

agcccccaga gcauccuguc uagcacccuc auucaucaau ccaucaccgg gcuguaugag     4080

acacgcauug accuuucuca gcugggcgga gauuga                               4116


<210>  4312
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4312
auggagaaca agaauuacag caucggccug gacauuggga ccaacuccgu gggauggucu       60

gucaucacag augacuauaa aguucccagc aagaaaauga aggugcucgg caauacggau      120

aaacacuuca uuaagaagaa ccugaucggg gcccuucugu uugacgaagg aaccacagcu      180

gaggccagac ggcucaaacg caccgcgaga cggcgcuaca caagacggaa gaaucgccug      240

agauaucuuc aggaaauuuu cuccgaggaa aucggcaaag uggauucuag cuuuuuccau      300

cggcuggacg auuccuuucu caucccugag gacaagcgcg ggucuaaaua cccgauuuuc      360

gcuacccugg ccgaggaaaa gaaguaucac aaacaauuuc ccacaaucua ccaucugaga      420

aagcagcucg ccgauagcaa agagaagacc gaccugcggc uuauuuaucu ggcucucgcc      480

cacaugauca aauaccgcgg acauuuccug uacgaagagg cuuuugauau uaagaacaau      540

gacauccaga aaauuuucaa cgaguuuauc uccaucuaua auaacacguu cgaaggcaau      600

agccuuucug gccagaacgu ccaaguggag gccauuuuua ccgauaagau cuccaagagc      660

gcgaaaagag aacggguucu gaagcucuuu ccugacgaga aaucuacagg gcuguucucc      720

gaauuucuga agcucauugu gggaaaucag gccgacuuca aaaagcacuu ugaucuggag      780

gagaaggcuc cccuucaguu cagccgcgac accuacgaug aagaccugga gaaccuccug      840

ggccagaucg gggaugacuu ugccgaucuu uuccuggucg cuaaaaagcu cuaugacgcc      900

auucugcugu cuggaauccu cacagugacc aauccuucca cgaaagcgcc ccugagcgcc      960

uccaugaucg aaagauacga gaaccaccaa aaggaucuug cuucucugaa acaguuuauu     1020

aagaacaauc ucccugaaaa guaugacgag guuuucagcg aucaguccga ggacggcuac     1080

gccggguaua ucgacggaaa aacaacccag gaaacauuuu acaaguacau uaaaaaccug     1140

cuuagcaagu ucgagggcgc ugauuauuuu cuggacaaaa ucgaacggga ggauuuccuc     1200

agaaagcaac gcaccuuuga caauggcucu auuccgcauc agauccaucu gcaggaaaug     1260

aacgccaucc ugcggagaca aggggagcac uaccccuucc ucaaggagaa uaaagaaaag     1320

auugagaaaa uccugaccuu ccgcauuccu uauuacgugg gaccccuugc ccggggcaac     1380

agagauuuug cuuggcugac acgcaauucc gaccaggcga uccggccuug gaacuucgaa     1440

gagauugucg auaaggccag cucugcugag gacuuuauca auaaaaugac gaacuaugau     1500

cucuaccugc ccgaagagaa ggugcugccg aaacacagcc uucucuauga aaccuucgcc     1560

guguacaaug agcugacaaa ggucaaguuu auugccgaag ggcugagaga cuaccaguuc     1620

cucgauuccg gacagaaaaa gcaaaucguu acccagcugu uuaaagagaa gcgcaaagug     1680

acagagaagg acaucauuca guuccuucac aacguggaug gcuaugacgg gaucgaacug     1740

aagggaauug agaaacaguu uaaugcuucu cucagcaccu accaugaccu gcugaagauc     1800

auuaaagaua aggccuucau ggacgaugcg aaaaacgaag cuauccuuga gaauaucguc     1860

cacacgcuca caauuuuuga agaccgggag augaucaagc aaagacuggc ccaguaugau     1920

ucccuguucg acgagaaggu gauuaaagcc cucacccgcc ggcauuacac cggcuggggc     1980

aagcugagcg cuaaacuuau caacgggauu ucugauaagc agacaggaaa caccauccug     2040

gacuaucuca ucgaugacgg cgaaauuaau agaaacuuua ugcagcugau caaugaugac     2100

gggcuguccu ucaaagagau uauccaaaag gcccagguug ugggaaagac aaacgauguc     2160

aaacaggugg uucaggaacu uccuggcagc cccgcuauua agaaagggau ccuccaaucu     2220

aucaagauug uggacgagcu ggucaaagug augggccacg ccccugaauc caucgucauu     2280

gagauggcgc gggagaauca gacgaccgcc cgcggaaaga aaaacagcca gcaaagauac     2340

aagcggaucg aagacucccu gaagauucuc gcuucuggcc ugaauagcaa aauccuuaag     2400

gagcauccca cagauaacau ucagcugcag aaugacagac ucuuccugua uuaccugcag     2460

aacgggaaag auauguacac cggagaagcc cuugacauca aucaacucuc cucuugcgau     2520

aucgaccaca uuauccccca ggcuuuuauu aaggaugaca gccuggauaa ccgcgugcug     2580

acauccagca aagacaaucg gggcaagucu gauaacguuc cuucccucga gguggucgac     2640

aagaugaaag uguucuggca gcagcugcuu gacagcaagc ugaucucuua uagaaaauuu     2700

aauaaccuca ccaaggccga gcgcggggga cugaacgaac uggauaaagu uggcuucauu     2760

aagagacaac uuguggagac gcggcagauc accaagcacg ucgcgcagau ccuggacgcc     2820

cgcuuuaaua aagaagugac agagaaggau aaaaagaaca gaaccgugaa aauuaucaca     2880

cucaagucca aucugguuag caacuuccgg aaggaauuug agcucuacaa aguccgcgag     2940

auuaaugacu aucaucacgc ucaugaugcc uaccugaacg cugugguggc caaggccauc     3000

cuuaaaaagu auccgaaacu ggaacccgag uucgucuacg gggacuauca gaaguacgau     3060

cucaagagau acauuucucg gagcaaagac ccuaaggaaa ucgagaaagc uaccgaaaag     3120

uauuucuucu acuccaaucu gcugaacuuu uucaaagagg aggugcacua ugcggauggc     3180

acgaucguua agcgcgaaaa uauugaguac agcaaagaca caggagaaau cgccuggaac     3240

aaggagaagg auuucgcuac cauuaaaaag gugcuuucuc ugccccaagu caauaucgug     3300

aaaaagagag aaguucagac aggcggguuu uccaaagaga gcauucuccc uaagggaaac     3360

uccgacaagc ugaucccccg gaaaaccaag gauauucucu gggacaccac aaaauauggc     3420

ggguucgacu cuccggugau cgccuacagc auccugcuua uugccgauau cgagaaggga     3480

aaagcuaaga agcugaaaac ggucaagacc cucgugggca uuacaaucau ggaaaaagac     3540

gccuuugaga agaauccuau ugcguuccug gaaaacaaag gguaucacaa uguuuguaag     3600

gagaacaucc ugugccuucc caaguacucc cuguuugagc ucgaaaacgg ccgcagacgg     3660

cugcucgcuu cugccaaaga gcugcagaag uguaaugaaa ucgugcuucc ugucuaccug     3720

accacacugc ucuaucauag caaaaacgug cacaagcugg augagcccgg acaucuugaa     3780

uacauucaaa aacacagaaa ugaguucaag gaccugcuca accugguguc cgaguuuucu     3840

cagaaauaug uccucgccga ugcuaaucug gaaaagauca agaaccuuua cgccgacaau     3900

gagcaggcgg auauugaaau ccuggcuaac agcuucauua aucugcucac cuuuacggcc     3960

cugggcgccc cugcugccuu caaauuuuuc gggaaggacg uugaucgcaa acgguauaca     4020

accguguccg agauccuuaa cgcuacccug auccaucaga gcauuacagg acucuacgaa     4080

accagaaucg accugucuaa gcucggcgag gauuga                               4116


<210>  4313
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4313
augaagguga acaauuacca caucggccug gacauuggga ccagcuccau cggauggguc       60

gccauuggcg aggaugggaa accccucaga aucaagggaa aaacagcuau uggcgcccgg      120

cuguuccagg aagggaaccc ugcggcugac cgcagaaugu uucggacgac ccgcagacgg      180

cuuucucgca gaaaguggcg gcugaaacuc cuggaggaaa ucuucgaucc guauaucaca      240

cccguugaca gcaccuuuuu cgcccgccuu aagcaaucca aucugucucc uaaggauagc      300

agaaaagagu uuaagggauc caugcuuuuc cccgaccuga cagauaugca guaccauaaa      360

gacuauccua ccauuuacca ucugcggcac gcccucauga cacaggauga aaaguuugac      420

auccgcaugg uguaucuggc uauucaccau aucgugaaau acagaggcaa cuuccuuaau      480

ucuacccccg ucgauagcuu uaaggccucc aaggugaacu ucguugacca guuuaaaaag      540

cugaaugagc ucuauacggc uauuaacccu gaggaauccu uccaaaucaa ucuggccaac      600

ucugaggaua uuggccacca guuucuugac ccguccaucc ggaaauucga caagaaaaag      660

cagaucccca aaauugugcc ugucagcgug gaugacaagg cgaccgauaa gaucaauggg      720

aaaauugccu cugaaaucau uaacgcuauc cugggauaca aguccaaacu cgacguugug      780

guccagugca cacccgugga uagcaagucu ugggcccuga aauucgacga ggaggauauc      840

gacgcuaagc ugcaaaagau ucucccugaa auggaugaga aucagcaguc caucauugcc      900

auccugcaga accuuuacag ccaagugacc cugaaucaga uuguccccaa cggcaugucc      960

cucucugaaa gcaugaucga gaaauauaac gaccaccaug aucaccugaa gcucuacaaa     1020

aagaucauug accagcuggc ggacccgaaa aagaaggccg cucucaaaaa ggccuauucc     1080

caauacguug gggaugacgg aaaagugauc gaacaggcug auuuuuggag cucugugaag     1140

aaaaaucugg acgauuccga ccugagcaag cagauuaugg aucucaucga cgccgagaag     1200

uucaugccua aacagcgcac aucucaaaac ggcgucauuc cccaucagcu gcaccagaga     1260

gagcuugaug aaaucauuga gcaccagagc aaguauuacc cuuggcuggc cgaaaucaau     1320

cccaacaaac augaccucca ccuggcuaag uauaaaaucg aggaacuugu ggcguuucgg     1380

guucccuacu acguggggcc uaugauuacc ccggaugacc aagccaaguc cgcugagacg     1440

gucuucucuu ggauggagag aaaaggaaag gaagccggcc agaucacacc cuggaauuuu     1500

gaugagaagg uggaccgcaa cgccagcgcu aaucgguuca uuaaaagaau gaccacaaag     1560

gacaccuauc ugaucggcga agauguucuc ccugacgagu cccugcugua cgaaaaauuu     1620

aaggugcuca acgagcugaa uaugguccgc gugaacggga aacuucugaa ggucgccgau     1680

aagcaggcga uuuuccagga ccucuuugag aauuauaaac auaucagcgu gaagaaacug     1740

caaaacuaca ucaagucuaa aaccggacug cccuccgauc cugaaauuag cggccuuucu     1800

gaccccgagu auuucaauaa cucccucggg acauacaaug auuuuaagaa gcuguucgga     1860

aacaaaguug acgaaccgga ucugcaggac gauuuugaga agaucgugga guggagcacg     1920

gucuucgaag acaaacggau ucucagagag aagcugaacg aaaucaccug gcuuuccgau     1980

cagcagaaag acgugcugga gucuagccgc uaucaaggcu gggggcggcu cuccaagaag     2040

cugcugacag gaauuguuaa ugaccagggc gaaagaauca ucgaugagcu uuggaacacc     2100

aauaaaaacu ucaugcagau ucaaucugac aaugauuuug cuaagcgcau ccacgaggcc     2160

aacgccgacc agaugaaagc uguggauguc gaagacgugc ucgccgaugc uuacacaagc     2220

ccucagaaua agaaagccau uagacaggug guuaaggucg uggacgauau ccaaaaagcg     2280

augggcgggg uggcccccaa guacauuucc aucgaguuca cccggagcga agaccgcaac     2340

ccucggagaa cgaucucucg gcagcgccag cuggagaaua cacugaagga uaccgcuaaa     2400

ucccucgcca agagcauuaa ccccgaacug cuuucugagc uggacaaugc ugccaaaucc     2460

aagaaaggac ucaucgacag acuguaucug uacuuuaccc agcuuggcaa ggauauuuau     2520

acagggaagc cuaucaacau ugacgagauc agcaccuacg auauugacca uauccucccc     2580

caagcguucg ucaaagauga cucucugaau aaccgggugc ugguuagcaa ggccaucaau     2640

aacggaaaau ccgauaacgu gccgguccag cucuuuggcg cuaagauggg gcacuucugg     2700

aaacagcugg ccgaagcugg ccuuauuagc aagagaaagc ugaaaaaucu ccagacagac     2760

ccugauacga ucucuaagua ugccaugcac ggauuuauuc gccggcaacu gguggagacc     2820

ucccagguua ucaaacuggu ggccaacauu cuuggcgaca aguacagaaa ugaugacaca     2880

aaaaucaucg aaauuaccgc ucgcaugaac caucagauga gagaugaguu cggguuuauc     2940

aagaaucggg aaauuaacga cuaucaccau gcguucgacg ccuaccucac agcuuuucug     3000

ggacgcuacc uguaucacag auacaucaag cuccggcccu auuucgucua cggcgauuuu     3060

aaaaaguuca aagaggacaa ggugaccaug cgcaauuuca acuuucugca cgaucuuacg     3120

gacgauaccc aagagaaaau ugccgacgcc gaaacagggg agguuaucug ggauagagaa     3180

aauagcaucc agcagcugaa ggacguguau cauuacaagu ucaugcucau uucccacgag     3240

gucuauaccc ugcggggagc uauguuuaac cagacagugu accccgccuc ugaugcgggc     3300

aaacgcaagc ugaucccuau uaaagcugac agaccgauca auguguacgg gggcuauagc     3360

ggauccgccg augccuacau ggcuauuguc cggauccaua acaagaaagg cgacaaguau     3420

cgcguugugg gggugcccau gagagcccgg gauagacuug acgcggcuaa aaaggucucu     3480

gacgccgauu acgaccgcgc ccugaaggau gugcucaccc cucaacugac gaaaacaaag     3540

aaaagccgga agaccggaga gauuacacag guuguggaag acuucgagau cguccucggc     3600

aaagugaugu auagacagcu gaugaucgau ggggacaaga aguuuaugcu uggauccucu     3660

accuaccagu auaaugcuaa acaacugguu cucagcgauc aguccgugaa gacccuggcc     3720

agcaaaggcc gccuggaccc ccuucaggaa ucuauggauu acaacaaugu cuacacagag     3780

auucuggaca aggugaacca guauuucucc cucuacgaua ugaacaaauu ucggcacaag     3840

cugaaucucg gguucagcaa guuuaucucu uucccuaacc auaauguuuu ugacggcaac     3900

acgaaagcua gcagcggaaa gagagaaauu cuggaggagg ugcuuaaugg ccugcacgcc     3960

aaccccaccu ucgggaaucu caaagacauc ggaauuacaa ccccuuucgg ccaacugcag     4020

cagcccaacg ggauccugcu uucugaugaa gcgaagaucc gcuaucaguc cccgacagga     4080

cuguuugagc ggaccgucag ccucaaagac cuguga                               4116


<210>  4314
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4314
augaagguga acaauuacca caucggccug gacauuggga ccagcuccau cggauggguc       60

gccauuggca aagaugggaa gccccucaga guuaaaggaa agacagcuau cggcgcccgg      120

cuguuccagg aggggaaccc ugcggcugac cgcagaaugu uucggacgac ccgcagacgg      180

cuuucucgca gaaaauggcg gcugaagcuc cuggaagaga uuuucgaucc guauaucaca      240

cccguggaca gcaccuuuuu cgcccgccuu aagcaaucca aucugucucc uaaagauagc      300

agaaaggaau uuaaaggauc caugcuguuc cccgaccuga cagauaugca guaccauaag      360

aacuauccua ccaucuacca ccuccggcau gcccucauga cacaggacaa aaaguuugau      420

auucgcaugg uguaucuggc uauccaccau auugucaagu acagaggcaa uuuccuuaac      480

ucuacccccg uggacagcuu uaaagccucc aagguugauu ucguggacca guuuaaaaag      540

cugaaugagc ucuaugcugc caucaacccu gaagagagcu ucaaaauuaa ucuggcgaac      600

ucugaggaua ucggccacca auuucuugac ccguccauuc ggaaguucga caaaaagaag      660

cagaucccca aaaucguccc ugugaugaug aaugauaagg uuacggaccg ccugaacggg      720

aaaauugcca gcgaaaucau ucacgcuauc cucggauaca aggccaaacu ggaugugguc      780

cugcagugca cccccgugga cucuaagccu ugggcucuca aguucgauga cgaggauauu      840

gacgccaaac uggaaaagau ccuucccgag auggaugaga aucagcaauc caucguggcg      900

auucugcaga accucuacag ccaggucaca cugaaucaga ucguuccgaa cggcaugucu      960

cuuuccgaaa gcaugauuga gaaauauaac gaccaucacg aucaucugaa gcucuacaaa     1020

aagcugaucg accaacuggc cgacccuaag aaaaaggcug ugcucaaaaa ggccuauucc     1080

caguacgugg gggaugacgg aaaagucauu gaacaggcug aguuuugguc uagcgugaag     1140

aagaaucugg augacuccga acuuagcaaa caaaucaugg aucugaucga cgccgagaag     1200

uucaugccca aacagagaac cucucagaac ggcguuauuc cucaccagcu ccaccaacgg     1260

gagcuggaug aaaucauuga gcaucagucc aaguauuacc ccuggcuugu ggaaaucaau     1320

cccaacaaac acgaccugca ucucgccaag uauaaaauug agcagcuggu cgcuuuuaga     1380

gugccuuacu acguugggcc gaugaucaca cccaaggauc aggcggaaag cgccgagacc     1440

guguucucuu ggauggagcg caagggaacg gaaacaggcc aaauuacccc uuggaauuuu     1500

gacgagaaag ucgaucggaa ggcuagcgcc aacagauuca ucaaacgcau gacaaccaag     1560

gacaccuauc ugaucggcga agaugugcuc cccgacgagu cccugcuuua cgaaaaauuu     1620

aagguccuga augagcucaa cauggugcgg guuaauggga agcugcugaa aguggccgac     1680

aagcaggcua uuuuccagga ucuuuuugag aacuauaaac acgucucugu gaagaaacuc     1740

cagaauuaca ucaaggccaa gacaggacug ccuagcgacc ccgaaauuuc cggccugagc     1800

gauccggagc auuucaacaa uucucucggg acguauaacg acuuuaaaaa gcuguucgga     1860

uccaaaguug augaaccuga ccuucaagau gacuuugaga agaucgugga guggagcacc     1920

gucuucgaag auaaaaagau ucugagagag aagcucaaug aaaucacaug gcugucugac     1980

cagcagaaag augugcugga guccagccgc uaccagggcu gggggcggcu uuccaagaaa     2040

cuccugaccg gaaucgugaa cgaccaaggc gaaagaauua ucgauaagcu guggaacaca     2100

aauaaaaacu ucaugcagau ucagucugac gacgauuuug cgaagcgcau ccacgaggcu     2160

aaugccgacc aaaugcaggc cguugauguc gaggacgugc ucgcugaugc cuauaccagc     2220

ccccagaaca aaaaggcuau uagacaggug gucaaggugg uugacgauau ccaaaaagcc     2280

augggcgggg uggcgccuaa guacaucucc auugaguuca cgcggucuga agaccgcaau     2340

ccccggagaa caaucagccg gcagcgccag cuggaaaaca cccuuaaaga uaccgccaag     2400

ucccuggcua aaagcauuaa uccugagcuc cugucugaac uggacaacgc cgcuaagucc     2460

aagaaaggac uuacagauag acucuaccug uauuuuaccc agcugggcaa ggacaucuac     2520

acaggggagc ccauuaauau cgacgagcuc aacaaauaug auaucgacca cauucugccg     2580

caagccuuca ucaaggauaa uagccuugac aaccgggucc uggugcucac ggcgguuaau     2640

aacggaaaau cugauaaugu gccucugaga auguuuggcg ccaagauggg gcauuucugg     2700

aagcagcugg cugaagccgg ccuuauuucc aaacgcaagc ucaaaaaccu gcagaccgac     2760

cccgauacaa ucagcaagua cgcuaugcac ggauuuauuc ggagacagcu ggucgagacc     2820

ucucaaguga ucaaacucgu ugccaacauu cugggcgaca aguaucgcaa ugaugacaca     2880

aagaucaucg aaauuaccgc cagaaugaac caucagaugc gggaugaguu cggguuuauc     2940

aaaaaucgcg aaauuaacga cuaccaccac gcuuucgaug cguaucuuac ggccuuucug     3000

ggaagauacc ucuaccaucg guauaucaag cugcgccccu acuucgugua uggcgacuuu     3060

aaaaaguuca gagaggacaa agucaccaug cggaauuuca acuuucugca cgaucuuaca     3120

gacgauaccc aggagaagau ugcugacgcc gaaacagggg aggugaucug ggaucgcgaa     3180

aauagcaucc aacagcugaa ggacguguac cauuauaaau ucaugcucau uucccacgag     3240

gucuacaccc ugagaggagc cauguuuaac cagacgguuu auccugcuag cgaugccggc     3300

aagcggaaac ucaucccggu gaaggcggac cgccccguga augucuacgg gggcuacucu     3360

ggauccgcug augccuauau ggccauugug agaauccaua acaaaaaggg cgacaaauac     3420

cggguugugg gggucccuau gagagcucug gaucgccuug acgccgcgaa gaaugugagc     3480

gaugcugacu ucgaccgggc ccugaaggau guucucgcuc cccagcugac aaaaaccaag     3540

aaauccagaa agacaggaga gauuacccaa gugaucgaag acuuugagau cguccugggc     3600

aaagugaugu aucgccagcu uaugauugau ggggacaaga aguucaugcu gggaucuagc     3660

accuaccagu auaacgccaa acagcucguu cuguccgauc aaucugugaa gacacucgcc     3720

agcaaaggcc ggcuggaccc ucuucaggaa uccauggauu acaauaacgu cuauacggag     3780

auccuggaca aggugaauca guacuuuucu cucuacgaua ugaacaaauu cagacacaag     3840

cugaaccugg gguuuagcaa guucauuucc uuucccaauc acaacgugcu ugacggcaau     3900

accaaaguca gcucuggaaa gcgcgaaauc cugcaggaga uucucaacgg ccugcaugcu     3960

aauccuacau ucgggaaccu caaagauguu ggaaucacca cacccuucgg ccaacugcag     4020

cagccgaaug ggauucuucu guccgacgag accaagaucc gguaucagag cccuacggga     4080

cuguuugaaa gaacaguguc ucucaaagac cuguga                               4116


<210>  4315
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4315
auggacaaga aauacagcau cggccuggau auugggacca acuccguggg augggccguc       60

aucacagacg auuauaaggu ucccucuaaa aaguucaaag ugcucggcaa uacggacaga      120

cacagcauua agaagaaccu gaucggggcg cuucuguuug auuccggaga gaccgccgaa      180

gcgacacggc ucaaacgcac cgcuagacgg cgcuacacaa gacggaagaa ucgcauuugc      240

uaucugcagg agaucuucuc uaacgaaaug gccaaagugg acgauagcuu uuuccauaga      300

cuugaggaau ccuuucuggu cgaggaggac aagaaacacg aacggcaucc uaucuucggc      360

aauauugugg augagguugc cuaccacgaa aaguauccga ccaucuacca ucuccgcaag      420

aaacuggcug acucuacaga uaaggccgac cugagacuca uuuaucuggc ucuugcccac      480

augaucaaau uucgggggca cuuccugauu gagggagauc ucaaccccga caauagcgac      540

guggauaagc uguuuaucca acuuguccag accuacaacc agcuguucga ggaaaauccu      600

auuaacgcgu cccgcgugga cgccaaagcu auccucagcg ccagacuguc uaagucccgg      660

cgccuggaga aucucaucgc ucagcugccc ggcgaaaaga agaacggccu uuuugggaau      720

cugauugccc ucagccuggg acuuacgccu aacuucaagu cuaauuuuga ucuggcggag      780

gacgccaaac uccaacuguc caaggauacc uacgacgaug accuggauaa ccuccuggcu      840

cagaucggcg accaguaugc cgaucuuuuc cuggcugcca aaaaccucag cgacgccaca      900

cugcuuucug acauucugag aguuaauucc gaaaucacca aggcuccccu cagcgcgucc      960

augauuaaac gguacgauga gcaucaccag gaccugacac ugcucaaggc ccuggugaga     1020

caacagcuuc cugagaagua uaaagaaauc uucuuugauc agucuaagaa cggguacgcu     1080

ggauauaucg acggcggggc cagccaggag gaauuuuaca aauucauuaa gccgauccug     1140

gagaaaaugg auggaaccga ggaacuccug gccaagcuga aucgcgagga ccuucuccgg     1200

aagcaaagaa cguucgauaa cggcuccauu ccccaucaga uccaccuggg cgaacugcac     1260

gcuauucucc gccggcagga ggacuuuuau ccuuuccuga aagauaauag agaaaagauc     1320

gagaaaaucc uuacauuucg cauucccuac uacgucgggc cucuggcccg gggaaacagc     1380

agauucgcgu ggaugacccg caagucugaa gagacaauca cccccuggaa uuuugaggaa     1440

gugguggaca aaggcgcuuc cgcccaaucc uucauugagc ggaugaccaa cuuugauaag     1500

aaucucccga acgaaaaggu ccugccuaaa cauucucugc uuuaugagua cuucacaguu     1560

uauaaugagc ucacgaaggu gaaauacgug accgaaggga ugagaaagcc cgccuuucug     1620

agcggagagc agaaaaaggc uaucgucgac cuucucuuca aaacaaaccg caaggugacc     1680

guuaagcagc ugaaagaaga uuauuucaag aaaauugagu guuuugacuc cguggaaauc     1740

ucuggcgucg aggaccgguu caaugccagc cuugggacau accacgaucu gcucaagauu     1800

aucaaagaca aggauuuucu ggacaacgag gaaaaugagg auauccugga agacauugug     1860

cuuacccuca cgcuguucga ggauaaggaa augaucgagg agagacugaa aacauaugcu     1920

caucucuuug acgauaaggu uaugaaacag cugaagcggc gccacuacac cggauggggc     1980

agacuuuccc ggaaacugau uaacggcauc agagacaagc aaagcgggaa gaccauucuc     2040

gauuuccuga aaucugacgg auuugccaac cgcaauuuca uccagcugau ccaugaugac     2100

ucccuuacau uuaaggaagc gauucagaaa gcccagguga gcggccaagg gcacucucuc     2160

cacgagcaga ucgcuaaccu ggccggaucc ccugcuauua agaaaggcau ccugcagagc     2220

gucaaggugg ucgacgaacu cgugaagguu auggggcaua aacccgagaa uauugugauc     2280

gaaauggccc gggagaacca gaccacacaa aagggccaga aaaauuccag agagcgcaug     2340

aagagaaucg aagagggaau uaaagaacug ggcucucaga uccuuaagga gcaccccguc     2400

gagaacacgc agcugcaaaa ugaaaaacuc uaccuguauu accugcagaa cgggcgggau     2460

auguaugugg accaggagcu ugauauuaau cgccugagcg acuacgaugu ugaccauauc     2520

gugccucaau ccuucauuaa ggaugacucu aucgauaaca agguccucac cagaagcgac     2580

aaaaaucggg gaaaguccga ugacgugccg agcgaagagg ugguuaaaaa gaugaaaaac     2640

uauuggcgcc agcugcucaa ugcgaagcug aucacacaga gaaaguuuga caaccuuacc     2700

aaagccgaac ggggcgggcu gucugagcuc gauaaggcug gauucauuaa acgccagcug     2760

gucgagacaa gacaaaucac caagcacgug gcccagauuc uggacucccg gaugaauacg     2820

aaauacgaug aaaacgacaa gcugauccgc gaggugaagg ucauuacccu gaaaagcaag     2880

cucgugucug auuuuagaaa agacuuccag uucuauaagg uucgggaaau caacaauuac     2940

caccaugcuc acgaugccua ccugaacgcc guggucggca cagcucucau uaaaaaguau     3000

cccaagcugg aguccgaauu uguguacggg gacuauaaag uuuacgaugu gagaaagaug     3060

aucgcgaaaa gcgagcagga gaucggcaag gccaccgcua aacgcuucuu uuauucuaau     3120

auuaugaacu ucuuuaagac agaaaucacc cuugccaaug gagagauucg gaagagaccu     3180

cugaucgaaa cgaacgagga aacaggcgag auugucuggg acaaagggcg cgauuucgcc     3240

accgugcgga agguucucag caugccccaa gugaauaucg ucaaaaagac agaggugcag     3300

accggagcuc ugaccaacga auccaucuac gccagaggca gcuuugacaa acugauuucu     3360

cgcaagcauc gguucgaguc cagcaaauau gggggauuug gcuccccuac agugacguac     3420

ucuguccuug uuguggcuaa gagcaaggug caggauggga aagucaagaa aaucaagacc     3480

ggcaaagaac ugauuggaau cacacuccug gacaagcucg uguucgagaa gaauccccug     3540

aaauuuauug aggacaaggg cuacgggaac guucagaucg auaaaugcau caaacuuccg     3600

aaauauuccc uguucgaguu cgaaaaugga accagacgca ugcuggcuuc ugugauggcc     3660

aacaauaaca gccggggcga ccuccaaaag gccaaugaaa uguuucugcc ugcuaaacuu     3720

gucacacugc ucuaccacgc ccauaagauu gaguccucua aggaacugga gcacgaggcg     3780

uauauccucg aucacuacaa cgaccuguau cagcuucuga gcuacauuga aagauucgcu     3840

ucccuguaug uggauguuga gaaaaacauc agcaagguga aagaacucuu uucuaauauu     3900

gaguccuaca gcaucucuga aauuuguucc agcgucauca accugcuuac ccugacggcc     3960

ucuggggccc ccgcugacuu caaguuucuc ggaacaacca ucccucggaa gcgcuacggc     4020

agcccccaga gcauucuguc cagcacccuc auccaucaau cuauuacagg gcuguaugag     4080

accagaaucg aucuuagcca gcugggcucc gacuga                               4116


<210>  4316
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4316
augaacaaug aguacuucgu gggcuuugac aucgggacca acagcgucgg agccgcuguu       60

acagaugaaa aguaucacgu gcugaaauuc aagggcaaug ccauguggac guccgcggug      120

uuugagucug cucagcaaag cgccgacaga cggcucaacc gcaccgccag acggcgccug      180

gauagauccc agcagcggcg cgaccuucug caggaacuca uugcucccgc caucacaccu      240

gucgauccga gauucuuuga ccggauucgc gagucugcuc ugugggccga agauaaaacc      300

acagggagca gauucaccua cuuuuccgac gagggauaua cagauaagca auaccauaaa      360

gaguauccca ccauccacca ucuuauuugc ucucugauga augacccugc gccccacgau      420

ccucggcucg uguacaucgc cuguagcuau augcugacgc aucgcggcca cuuccugaac      480

gaagcugaca aggagaagau cgaugacguu cucgacauua gacggguguu uaaugaacug      540

ugcuccuggu ucaccagcua cgggaucgag guccccuuuu cuuguuuccc ugaggaauuu      600

ggaaacgugc uuacagccaa ucgcggcguu caggauagaa cccgggcuuu cuacgaccuc      660

cuguucgccg gcaaaaagcc gaaacccucc gaggaugacc agguggauau ugacguccug      720

aucaaacuua uuagcggggg aaaaaucgaa cugucuaagc ucuuucugaa cgaggaauau      780

uccgagcugg agaauaacaa gauucagcuc agcgaugcgg gcuucgacga aaaagagccu      840

gaacugagau cugagcuugg ggaugacuuu ggacuggugg ccgcugccaa gaauaucuac      900

gauugguccc uccugacagg cgugcuuggg gaaugcaaaa gcaucuccga ggcuaagguc      960

aaaaccuaug agaagcacuc ugaagaccug gccgcccucc gccggguugu gagaaaauac     1020

caucccgaca aguauaagga gauuuucaaa aaguacgugg ucggaaacua uacgagcuac     1080

uccggcaaug ugaaaagcgc uggcaagauc ucugaaacaa aacugaagcg cuguaaccaa     1140

gcggaguuuu gcgauuaccu gaaguccaaa cucaaggacg uuaaaccugc ccccggggau     1200

gacgaaugug agcgggugcu gaaggagauu agcaaacaca ccuucugccc gagacagguc     1260

acaaccgaua accgcgugau cccuuaucag cuuuacuaua ccgaacugaa gaagguucuc     1320

gagaaugcua aacgguacau uccccagcug aacgugacag acgaauaugg aucugccgcc     1380

gauaagaucc uuagccugau gacguuuaga aucccuuacu augucggccc ccucgugaaa     1440

aagaaugacu ccucuaacgc uuggauuguc cgcagagggg agggaagaau ccugcccugg     1500

aauuucgaug agcuggugga caaagauaag ugugaagacg aguuuauucg ccggaugacc     1560

ugcaagugua cauacaucgc cggcaaaaac guucucccua agaauagccu gcuuuacugc     1620

aaauucaugg ugcugaacga aauuaauaac aucaagauca auggggagag aauuuccguc     1680

ggagauaaac aaaagaucua uaccgaacuc uuugagaaaa agcggaaggu gacaguucgc     1740

gacauuagaa accugcugac cagcgagggc cggcuuaaag cgggcgacac guucggggga     1800

aucgauauua cagugaaguc uucccucaaa ccgcagcaug accugaagag acugauggcu     1860

gccggcguca ucaccgaagc cgaugcugag gaaauuaucg agcgcauuac cugcacaacc     1920

gacgugagcc ggcucagaac auggcugggg aaauuuggac uuucugccgc ugaugccaag     1980

uacguguccg gccugaagua uagcgacuuc gggcgccucu ccagagaguu ccugacggaa     2040

guuaaugaug ucgaccccgc gaccggcgag gugaucaacc cuaauaucau uacaaugaug     2100

ugggaaaaca acaucaaucu gauggagcuu cucucucaga aauacagcua ucggucccaa     2160

auugaaucua ugaaccgcga guacuaugcc gcuaaucccc ugacccugga ugaaagaaug     2220

agcgagaugu acaucccuuc cgccgugcgg cgccccguca agagaacaau cgagaugaug     2280

aaagaauaca ugcacauuau gcggaagccg ccuaaacgca ucuuuauuga gauggcuaga     2340

gaacucaccg accuggauaa caagggaaag cggacgagcu cucgcagaaa uacccuucug     2400

gaguucuuua aaaagugucc ccaggacaca accgagcucc gggcccagcu ggaaaagaag     2460

uccgaugacg agcugagaag cgaaaaacuu uaucucuacu ucgcgcagcu gggcaagugc     2520

auguauucug gggaguccau cgacauugaa cgccugggaa gcaagcucua cgauguggac     2580

cacaucuuuc cucaagcccg gguuaaagau gacucuaucg agaacaaggu gcugguccuu     2640

agcgaucuga auggcgcuaa auccgacaga uaucccauua acgccgauau ccgcaauaag     2700

augcauagcu ucugggacaa acuccacucu auugggcuga ucggagagaa gaaauacgaa     2760

cggcugacaa gauccaccgg cuuuacggau gacgagcuug cuggguucau uagccgccag     2820

cucguggaaa cacggcaguc caccaaggcc guugccgcuc ugcuggcgga ggaauuuccu     2880

gagucugaga ucguguaugu caaggccggc cucgcuagcc aguucagaca ugaauuugga     2940

cugcuuaaau gucgcgagau caacgaucug caccaugcca aggacgccua ccucaauauu     3000

gugaugggca acaucuacca cguuaaauuc acaaaggacc ccaugaauuu ugugaaagaa     3060

uaucggagaa aggagcaagu cuucgaucug aagauucagu ccaugcugac ccacgacgcu     3120

auccggaacg gggaaaccgc cuggauugga gauggcacau ggcuugagac ggugaaaaag     3180

cagauggaga aaaauaacau ccgcuacgug agauauucuu uccggcgcaa gggggcgcug     3240

uuuaaccuca ugccggaacg gaaaaauccu agccugcucc cccggaagaa cggaaagaau     3300

augguccugc ccgcugagaa auacggcggg uauaacaaua ccgccgccuc cuucuuuucu     3360

cuuguuaagu accauagcgc uaaaggcucc ggagugguga uuaucccugu cgaacugauc     3420

cacgcccaua gauaugagca cgacagcucu uucgcggagg aauacgcucu ccgcgugcug     3480

gccgauauug uuucccugaa gaaaggcgac gugcuucaga gcgucgaguu uccgcugggg     3540

ucucggaucc ucaaggugaa cacacugcuc gauauugacg gauucagagc caauaucacc     3600

ggcaaggauu ccgggggaaa auaccugggc auuagcucug cuuccagccu uaucaucucc     3660

caaguugaca caaccuauau uaagcgcgug aucagcauua acgaacggau cucugccugc     3720

gagaaaaaua agcagagcua cacgauuaga cccgaauuug augucaucac auccgagcgc     3780

aacaucgaga uuuauaauau gcugugugag aaaaucucua gccggccuuu cugcauuaag     3840

uuugcuaaca ccuccgacac cuucgugucu gggagagaaa aguuuaucgc ccucagccug     3900

aaugaacagg uuaaaacacu gaccaacaug cuuuccgugu ucaagacagg ccgcgcgucu     3960

ggaugugauc ugacgaauau uggcgggagc aaaaacaccg ccaucgucaa gcucaacgcu     4020

gugcugucca aucucaaaga cuucaagagc gugagaauua ucgaccaguc ucccacagga     4080

cuguacgaga aagucucccc uaaccuucug agccuguga                            4119


<210>  4317
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4317
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucaucacgg augacuauaa gauucccgcu aaaaagauga aaguucucgg caacaccgau      120

aaggaguaca ucaagaaaaa ucugcuuggg gcccuccugu ucgacucugg agaaacagcg      180

gaggcuacca gacugaagcg gacagcccgc agacgguaua cccgcagaaa gaaccggcuu      240

cgcuaccugc aggaaauuuu ugccgaggaa aucgcuaagg ccgauaauag cuucuuucaa      300

agacucgacg aguccuuccu gacagaugac gauaaagacu uugauucuca cccuaucuuc      360

ggcaacaagg cugaggaaga cgccuaucau cagaaguuua gcaccauuua ccaucugcgg      420

aaauaucucg cggauucccc ggagaagucu gaccugcgcc uuguguaccu ggcccucgcu      480

cacaugauca aauacagagg gcacuuccug auugaaggag agcuuaaggc cgagaauacg      540

gacgugcaga aacuguuuac cgauuucguc gaaguguaug accggacauu ugaugacagc      600

caucucuccg agguuaccgu ggaugcuagc ucuauccuga cagaaaagau uuccaaaagc      660

cgcagacugg agaaccucau cgaaucuuac cccaaggaga agaaaaauac ccuguucggc      720

aaccuuauug cccugucccu cggccugcag ccuaauuuua agacgaacuu caaacuuagc      780

gaggacgcga agcugcaauu cucuaaagau acauaugaag aggaccucga agagguccug      840

gaaaagaucg gggaugacua cgccgaucug uuuaccuccg cuaagaaucu cuaugacgcc      900

auccugcuua gcggcauucu gacaguggac gauuccucua ccaaagcucc ccucagcgcc      960

uccaugauca agcgguacgc cgagcaccac gaggaccugg aaaaacuuaa ggcuuucauu     1020

aaaaccaaca agccuaaucu guaucaugau aucuuuaagg acgagacaca gaacggauac     1080

gcgggguaca uugaaaacgg aguuaaacag gaugaguucu auaaguaccu caaaacgauc     1140

cugagcaaga ccgacggcuc ugaauauuuu cuggauaaaa ucgagcgcga ggacuuccuc     1200

agaaagcagc ggacauuuga uaaugggucc auuccccacc aaauccaucu gcaggaaaug     1260

cacgccauuc uuagacgcca gggagacuac uauccuuucc ugaaggagaa ccaggaucgg     1320

aucgaaaaga uucucaccuu uagaaucccg uacuaugugg gcccccuggc ucgcaagaau     1380

agccgguucg ccugggccga guaccacucu gacgagaaaa ucacaccuug gaacuuuaau     1440

aaggucauug auaaagaaaa gagcgcugag aaauucauca ccagaaugac gcugaacgac     1500

cuuuaccucc ccgaagagaa ggugcugccu aagcauuccc acguguauga aacauuugcc     1560

gucuacaaug agcugaccaa aauuaaguau guuaacgagc aaggcaaaga agcguucuuc     1620

gacucuaaua ugaagcagga gaucuuugau cauguguuca aagaaaaccg caaggugacc     1680

aaggagaaac uccugaauua ccuugacaag gaauuucccg aguaucggau ucaggaucug     1740

aucgggcucg acaaagagaa caagagcuuc aaugcuuccc ugggaacaua ccacgaucug     1800

aaaaagauuc uugacaagag cuuucucgau gacaaaguca acgaagaggu gaucgaagau     1860

aucauuaaga cccugacacu guucgaggac agagaaauga uccaacagcg ccuccauaaa     1920

uauucugaua ucuuuuccac gcagcagcug aagaaacuug agcggagaca cuacaccggc     1980

ugggggcgcc ugagcuacaa gcucauuaau ggcauucgga acaaggagaa caauaaaaca     2040

auccuggaau aucugaucga cgauggauac gccaacagaa auuucaugca acuuauuaac     2100

gacgacaccc ucccguuuaa gcagaucauu aaagaugccc agaucauugg gaauaucgac     2160

gauaucgcug ccguugugca cgagcugccu ggaucucccg cuauuaagaa aggcauccug     2220

caguccauua agaucgucga cgaacucgug aaaguuaugg gccauaaccc ugauaauauu     2280

gugaucgaga uggcccggga gaaccaaaca accaaucgcg ggagaagcca gucccagcag     2340

cggcugaaga aacuucaaga cucucugaaa gaacucggaa gcaacauccu gaauaagaaa     2400

aagcccuccu augucgagga uaaaguggaa aacguccauc ugcaggacga uagacuuuuc     2460

cucuacuaua uucagaaugg caaggacauc uacacggggg aggaacugga uauugaccgc     2520

cugucugacu augauaucga ccacauuauc ccccaggcgu uuauuaagga ugacagcauc     2580

gauaaccggg ugcucaccuc cagcgccaaa aauagaggaa agucugacga uguuccuaac     2640

cuggacaucg ugcgcgcuag aaaagccgau uggguccggc uuuacaaguc cggccugauu     2700

agcaaacgca aguucgacaa ccucacaaag ucugagagag ggggccugac cgaggcugau     2760

aaagccggau uuaucaagcg gcaacuggug gaaacacgcc agauuaccaa acacguugcg     2820

cagauccuug acgccagauu caauacggag cacgaugaag acgacaaggu gauucggaac     2880

gucaaaguga ucacacugaa guccaaucuc aucagccaau uccgcaagga uuuuaaauuc     2940

uacaagguga gagagauuaa cgacuaucau cacgcucaug augccuaccu gaaugcuguu     3000

gucggcaccg cccuccugcg gaaauauccg aaacuugccc ccgaauuugu guacggggag     3060

uauaaaaagu acgacgugcg caagcugauc acaucuagcu ccgauaaagc uaccgcgaag     3120

uauuucuuuu acagcaaccu caugaauuuc uuuaaaagag ucgugcggua cucuaacgga     3180

agaguuauug ugcgcccugu caucgaguau uccaaggaca ccggcgaaau uguguggaau     3240

aaagagacag auuuccggac gaucugcaag guucugagcu acccccaggu gaacaucguc     3300

aaaaaggugg aaucccagac cgggggauuu ucuaaggaga gcauucugcc uaaaggcgac     3360

uccgauaaac uuauccccag aaaaacaaag aaaguuuauu gggacaccaa gaaguacggg     3420

ggcuucgauu cuccgauuau cgccuauagc guguuugucg uggcugacgu ggaaaaagga     3480

aaggccaaaa agcugaaaaa ggucaaggag cucguuggca uuuccaucau ggagcgcccu     3540

ucuuucgaaa agaaucccau uguguuccug gagaagaaag gguaccggaa cgugcaggaa     3600

gauacacuca ucaagcugcc uaaauauagc cuuuuugagu ucgagggagg cagacgccgg     3660

cugcucgccu ccgcuagcga acugcaaaag gggaaugagg ucgugcuguc ugacagacuu     3720

guugaacugc ucuaccacgc ccaucgcgug aacuccuuua auagcaccga gcaccugaag     3780

uacgucucug aacacaaaaa ggaguucgga gaggugcucu ccuguguuga aaacuuugcg     3840

aacagcuaug uggacgucga gaaaaaucug gauaagaucc gggcugugau uaacaaaauc     3900

gacacguucu cuauugaaga gaucuccacc agcuuuguua aucuucugac acugaccgcc     3960

cucggcgccc ccgcugauuu caacuuucug gggaagaaaa uuccuagaaa gcgguacaca     4020

uccaccaagg aaugccuuaa ugccacacug auccaucaga gcaucacggg ccucuaugag     4080

acccgcauug accugucuaa acucggagag gauaaguga                            4119


<210>  4318
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4318
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucaucacgg augacuauaa gguucccgcu aaaaagauga aagugcucgg caacaccgau      120

aagaaguaca uuaaaaagaa ucugcuuggg gcccuucucu ucgacucugg agagacagcg      180

gaagcuacca gacugaaacg gacagcccgc agacgguaua cccgcagaaa gaaccggcuu      240

cgcuaccugc aggagaucuu ugccaaugaa auugcuaaag uggaugagag cuucuuucaa      300

agacucgacg aauccuuccu gacagaugac gauaagaccu uugacucuca cccuaucuuc      360

ggcaacaagg ccgaggagga ugcuuaucau cagaaauuuc cgacgaucua ccaucugcgg      420

aagcaccucg ccgacagcuc cgaaaaagcg gaucugcgcc uugucuaucu ggcccucgcu      480

cacaugauua aguucagagg gcauuuucug aucgagggag aacuuaaugc cgagaacacc      540

gacgugcaga aaauuuucgc ugacuuuguu ggcguguaca aucggacauu cgaugacucu      600

caccugagcg agaucaccgu cgauguggcc uccauucuca cagaaaagau cagcaaaucu      660

cgcagacugg agaaccugau uaaguacuau cccaccgaaa agaaaaauac gcucuuuggc      720

aaccugaucg cgcuugcccu ggggcuccag ccuaauuuca agacaaacuu caaacugucc      780

gaggacgcua aacuucaauu uagcaaagau accuacgaag aggaccugga ggaacuccug      840

ggaaagaucg gcgaugacua ugccgaucug uucacaucug cuaagaaucu cuacgacgcc      900

auucugcuuu ccgggauccu gaccguugau gacaacagca ccaaagcucc ccucucugcu      960

uccaugauua agcgguaugu ggagcaccau gaagaccugg agaaacuuaa ggaauuuauc     1020

aaagcgaaca agagcgagcu guaccacgau auuuucaagg acaaaaauaa gaacggauau     1080

gccggcuaca ucgagaaugg ggucaaacag gaugaauuuu acaaguaucu caaaaacauc     1140

cuguccaaga uuaagaucga cggaucugau uacuuccugg acaaaauuga gcgcgaagau     1200

uuucucagaa agcagcggac auucgacaau ggcagcaucc cucaucagau ucaucugcaa     1260

gagaugcacg cuauccuuag acgccagggc gauuauuacc ccuuucugaa agaaaagcag     1320

gaccggaucg agaaaauucu cacguucaga aucccuuauu acguggggcc gcuggugcgc     1380

aaggauuccc gguuugccug ggccgaguau agaagcgacg aaaagauuac ccccuggaac     1440

uucgauaagg ucaucgacaa ggagaaaucu gcugaaaagu uuauuacacg caugacccug     1500

aaugaccuuu accucccuga ggagaaaguu cugcccaagc acucccacgu guacgaaaca     1560

uaugccgugu acaacgagcu gaccaaaauc aaguauguca augaacaggg aaaggagagc     1620

uucuucgauu cuaacaugaa acaagaaauu uuugaccaug uguucaagga gaaucggaaa     1680

guuacgaagg agaaacuccu gaacuaccuu aauaaggaau uuccugagua uagaaucaag     1740

gaucugaucg gccucgacaa agaaaacaag agcuucaaug cgucccuggg gacauaccac     1800

gaucugaaaa agauucuuga caaagccuuu cucgaugaca aggugaacga ggaagucauc     1860

gaggauauua ucaagacccu gacccuguuc gaggacaaag auaugauuca ugaacgccuc     1920

cagaaguauu cugacaucuu uacagccaac cagcugaaaa agcuggagcg gagacacuac     1980

accggauggg gccgccugag cuacaaacuc aucaauggga uucggaacaa ggaaaauaac     2040

aaaacaaucc uggauuaucu gauugacgac ggauccgcca auagaaacuu caugcaacuu     2100

aucaaugaug acacgcuccc cuuuaagcag auuauccaaa agagccaggu gguuggcgau     2160

guggacgaua ucgaggcugu cgugcacgac cugccgggcu cuccugccau uaaaaagggg     2220

auccugcagu ccgucaaaau uguggaugaa cucguuaagg ugaugggagg caaccccgac     2280

aauaucguca uugagauggc ucgggagaac cagaccacaa aucgcgggag aagccagucu     2340

cagcaacggc ugaaaaagcu gcagaacucc cuuaaggaac ucggaagcaa uauccugaac     2400

gaggaaaaac cuuccuacau cgaggauaag guggaaaauu cucaucugca gaacgaccag     2460

cuuuuccucu auuacauuca aaacggcaaa gauauguaua ccggggacga acuggacauc     2520

gaucaccuga gcgacuacga uauugaccau aucauucccc aggccuuuau caaggaugac     2580

uccauugaua auagaguucu cacaucuagc gcgaaaaacc gcggcaaguc cgacgaugug     2640

cccagccugg acaucguccg ggccagaaag gcugaguggg ugcgccuuua uaaaucugga     2700

cugaucucca agagaaaauu cgauaaucuc accaaggccg aacggggcgg gcugacggag     2760

gcugacaaag ccggauuuau uaagcgccag cugguggaaa ccagacagau cacaaagcac     2820

guugcgcaaa uucuugaugc ccgguucaac accgagcacg acgagaauga caaagucauc     2880

cgcgauguga aggugauuac acugaaaagc aaccucgucu cucaguucag aaaggacuuu     2940

gaguucuaca aagugcggga gaucaaugau uaccaucacg cucaugacgc cuaucugaac     3000

gcuguugugg gcaccgcccu ccugaagaag uacccuaaac uugccuccga auuugucuau     3060

ggggaguaca agaaauauga uauccgcaag uucauuacga auagcucuga caaagcuaca     3120

gcgaaguacu uuuucuauag caaccugaug aacuuuuuca aaaccaaggu gaaguacgcc     3180

gauggaacag uuuuugaaag accgaucauu gagaccaaug cugacggcga gaucgccugg     3240

aauaaacaga uugauuucga aaaggugcgg aaaguccucu ccuaccccca agugaacauc     3300

guuaagaaag uggagaccca gacagggggc uuuagcaagg aaucuauccu gccuaaggga     3360

gacuccgaua aacugauucc ccgcaagacg aaaaaggucu auugggacac caaaaaguac     3420

ggcggguucg auagcccuac aguggccuau uccguguucg ucguugcuga cguggagaag     3480

ggaaaagcca agaaacuuaa gaccgugaaa gaacuggucg gcaucucuau uauggagcgg     3540

agcuuuuucg aggaaaaucc cguggaguuu cucgaaaaca agggguacca caacaucaga     3600

gaggacaagc ugauuaaacu cccgaaguau ucccuguucg aguuugaagg aggcagacgc     3660

cggcuucugg cgucugcuag cgagcuccag aaagggaaug aaaugguucu gccuggcuac     3720

cugguggagc uucuguauca ugcccacaga gccgauaacu ucaauuccac agaauaccuc     3780

aacuacgucu cugagcacaa gaaagaguuu gaaaaggugc ugagcugcgu ugaggacuuc     3840

gcuaaucucu auguggaugu cgagaaaaac cuguccaaga uccgcgccgu ggcggacagc     3900

auggauaauu uuucuauuga agaaaucucc aacagcuuca ucaaucuucu gacccugacg     3960

gcucucggag cccccgccga cuuuaacuuc cugggcgaga agauuccucg gaaaagauac     4020

acaucuacca aggagugucu uaaugcuacc cugauccauc aguccauuac agggcucuau     4080

gaaacccgca ucgaucugag caaacucgga gaggaauga                            4119


<210>  4319
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4319
augaacagcg acaagagaga ggauuacuau gugggccugg acauggggac cggaucccuc       60

ggcggggccg ucacagauac gcaguaccau cugcuuaaag uuaagggaaa agacuucugg      120

uuugugcggg aauaugagac cgcuaagucu caacuggauc gcagaacaca ccggaucgcc      180

aaacgcagac uccagcggca caaggcgcgc auuggccuga ucagaagcua cuucgcugaa      240

gacauucuua agcaugaucc ccuguuuuau auccggcagg acaauuccaa auacuaucac      300

gaggauaagg acgugcgccu caccacaaaa gauucuauuu ucgccgaccc uggguacgga      360

gauaaggaau accuggacga caaagaguau ccgaccaucu uucaucugag acaggcccuc      420

cugcgggauc aaaucaagga cgaggaacgc uacagcagau uccuuuaucu ggcuauuauc      480

aacauguuug agcaccgggg ccacuuccuc cugaauacag aauccucuga ucuuaacgcc      540

gacaugauua aggaugucgg cgagcuggug augaaugcua ucugcggggu uagcgaguac      600

ggauccguga ccuauaaaga aauuaucgag auucucgaag acaagaacau cagccgcacg      660

gccaaaaagg aucagaucgc gucuaaaauu gacaucaaga aauccgagaa ggcccagaug      720

gaaagaauua agugucugug cggcauggag gcugaugccc gggucauguu ugagaucgaa      780

gcugacgaga aaauuaagau cgccuuccag caagcggccu uugaagauga gaaagaagag      840

aucgaggcug ugauugggga agacagauac cugaucauug agaauaugaa gcagcucuau      900

gacuacagcc agcugcaguc uguucuuaac ggauucgauu accuguccga cgcccgcgug      960

caaauguaug aaaaacauaa ggaggaucuc caucugcuua agaaagucua ccggaagaau     1020

cuggacagag aaaucuauga ucgcauguuu cggagcggcg aggagggguc uuacugugcu     1080

uauuccaaca gcaccaauuc cguggacucu cucgccgaag ccaaaaagua cagacgcaac     1140

augaaaaagc ggagccagaa ggaucuguau gacagaauua aaaaggaucu gaaagaggug     1200

cacgacgcug augcggacgc cauccgcaag gaaauggagc ucgagcgguu ccugcccaaa     1260

cagaucacag gagcuaaugg cauuaucccu aaccaagucc acagaaagga acuuaagaaa     1320

auucuggaga augccgaaca ccaucucgau uuccugaagg agaaagacga auccggcuac     1380

accguuagcg agcgcauccu uaugcuguuu ucuuccaaca uucccuacua uguggggccu     1440

gugggaagcg gcucuaagac agggugggcc aaaauccagg agagcggaca gguccucccc     1500

uggaacaugg aacagaagau ugacaucgag caaaccuccg aacgguucau cacgaaucug     1560

auuagagagu gcacauaccu gucuggcgaa aaagugcucc cuaagcagag ccugcuuuau     1620

gaguccuacu guguucugaa cgagaucaau aaccuccgga uucacgggaa gcgcaucgug     1680

ccggaacuga aacaggauau uuauaaggag gucuuucagg gauucagacu gggcaaaaag     1740

gugaccaaaa agaauaucug cacauaccuu aucaaccggg gccucauuac cgaagagagc     1800

gaaguuucug ggaucgacaa ggagauuaau gcuuaucugc ccuccuacgg aaaaauguac     1860

ggcguguuug gggagagacu gcgcgaagag gccguccaag aaaucgcgga gaagauuauc     1920

uauuacggaa ccaucuaugg cgaugcuaaa agcauguucc ggaagaaacu caaccaguac     1980

gugucugacg gguggaugac agaguaucag gccaagagaa uuuccggcua caaguuuaaa     2040

gauugggccc gccugagcag auccaugcuu ugucugucug gaugcgacaa gagcacgggc     2100

gagaaaaucu cccucauucg ggcuaugugg gaauauaauc ugaacuucau ggaacugauc     2160

uaccgcgagg auuuuaccuu ccagaaggaa cuugaggaga gacggaaagc ccucgggaag     2220

acacugcaag aauuucaguu cgaggaccug gaugaauacu auuacucugc uccugucaag     2280

cgcaugauuu ggcaggccgu gcaggcgcuc aaagagauca cccaaaucau gggacauaau     2340

cccaagagaa uuuuuaucga aaugacacgg accgaggagg aaaaaggcga gcaggggcgc     2400

aagaacagca gagaaaaacg gcugcuuaag auguaugagu ccauucgcga cgaaagaaau     2460

ugggccgcug agaucaaaaa cgccaaugcu agcggaaagc ugaacucuaa gaaacucuac     2520

cuguauuacc ugcagauggg ccgggauguu uauacggggg agccuauuga caucgaagau     2580

cuuuucgacg acuccagaua cgauauugac cacaucuacc cccgcagccu cacaaacgau     2640

aauaacaucg agaauaaccu ggugcugguc ucuaagacca uuaaucaaaa cgaaaagaaa     2700

aaugacuauc cggugccuga gaaaauccgg agaaacaaga agguucgcaa ucucuggcau     2760

acccugcaca aaaugggcuu caugaacgau gagaaguaca aucggcuuac auccaccgaa     2820

agacugagcg aggaccagcu cgccggauuu auugcgcgcc agcuggugga aacagcccag     2880

ggcgcuaaag ggaucgccga gcugaugaag gcuaugcuuc ccggaacgga uauugucuau     2940

gccaaagccc ggaacguguc ugacuucaga aaggaauaca agaugcucaa aagccgccug     3000

gugaaugauu aucaccaugc ucaagacgcg uaccugaaca ucguugucgg caauguguau     3060

uuuaugaagu ucacccggaa cccuaugaac uuugugaaaa cagaggccag acgggauggg     3120

cgcaauuacg acuacaaccu cuauaagaug uacuccaaag acaucauuag aaauggagag     3180

aaggcuugga ucgccaccag cgaacagggc cccgggacaa uucggcuggu caaggagacc     3240

augggccgca acacgcccau cauuaccaga caggccuucg aacagcgggg agagcuuuuu     3300

aaucugcaac cugugggcaa auauucugcu aagaaagaua acuacguucc gcucaagauc     3360

aaugacgaga aaaugcagga uguguccaag uaugggggau acacaagccu gaaccccucc     3420

uauuucaucu uuauugaaca cggcccugag aagaaaagaa agaaauguuu cgaagucauc     3480

cauucuuacu acgccgcgca gauuaagacc gagaaagacc ugaucgauuu ucuucugcag     3540

aagggguaua aaaauccccg cgugauuaac gcucggauca agaagaaugc ccucauuaaa     3600

uacaacggau auuuccugua caucaucggc auggacgcca gaaagaauau ugaauuuagc     3660

aacgcuacag ccaugugccu caaaaauaag uauauccaau acguuuguaa acuggagaag     3720

augaacaagg cgauucuucu guccgaaaaa cagaagacca accuccacug ggaugagaaa     3780

aucacgugcg agucuaaucu ggaacuguau cgcgagcuua cagaaaagca ucugcacagc     3840

auuuaccagc ggcacccuag auccaucggg aaaugccucg cugacggcga gggagccuuc     3900

aagcugcucg auaucgagga acaagugaag auuaucugug acauugucca guacaccucu     3960

uuccagcgcg gcguguuuag ccugaaaguu cuugggggac ccaaggaggu gggccggauc     4020

agaauuuccg ggaacaugac agaagccaaa gagugcaagc uggucaauua uagcaucacc     4080

ggaauguaca aaaccgaaau ggaucuccug aagaaguga                            4119


<210>  4320
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4320
auggagaaga ccuacagcau cggccuggac auugggacaa acuccguggg auggucuguc       60

aucacggaug acuauaaagu ucccgccaag aaaaugaagg ugcucggcaa uaccgauaaa      120

aaguacauua agaaaaaccu gcuuggggcu cugcuuuucg acagcggaga aacagccgag      180

gugaccagac ugaagcggac agcgcgcaga cgguauaccc gcagaaagaa ucggcuucgc      240

uaccugcagg aaaucuuugc uaaggagaug gccaaagucg augaauccuu cuuucaaaga      300

cucgaggagu cuuuccugac agacgaugac aagaccuuug auagccaccc uauuuucggc      360

aacaaggccg aagaggacgc uuaucaucag gaauuuccga cgaucuacca ucugcggaaa      420

caccucgccg auuccucuga gaaggcugac cugcgccuug uguaucuggc ccucgcgcac      480

augaucaaau ucagagggca uuuucugauu gagggagaac uuaaugccga gaacaccgau      540

auccagaagc uguucaauga cuuuguugaa acauacgaca aaauugcuga ugagagccac      600

cucuccgaag uggacgccag cucuauccug accaacaaga uuuccaaaag ccggcgccug      660

gagaaucuca ucaagcagua ccccacagag aagaaaaaca cccuguucgg caaucuuguc      720

gcucuggugc ucggccugca accuaacuuu aagacgaauu ucaaacuuuc ugaagaugcc      780

aagcugcagu ucuccaaaga cacauaugau gaggaccucg aggaacugcu ggggaagauu      840

ggagaugacu acgcggaucu cuuuaccgcc gcuaagaacc uguaugacgc cauccuucug      900

agcggcaucc ucacaguuga ugacaauucu accaaagcuc cccuguccgc cagcaugauu      960

aagagauacg aggaacacca ugaggaccuu gaaaaacuga agaaauucau caaggugaac     1020

aacuuugaua aguaucacga gauuuucaaa gacaagucca aaaaugggua cgccggauau     1080

aucgaaaacg gcgucaagca ggauauuuuc uacaaacauc ucaagucuau caucagcgag     1140

aagaaugggg gacaguacuu ccuggacaaa auugaacggg aggauuuucu gcgcaagcaa     1200

agaaccuucg acaacggcuc caucccucac cagauucacc uccaggagau gcgggcuauc     1260

cugagacgcc agggcgaaua uuaccccuuu cuuaaagaga aucaagcgaa gauugaaaag     1320

auccugacau uccggauccc uuauuacgug gggccgcucg ccagaaagga uagccgcuuu     1380

gcuugggcca acuaucauuc ugacgagccc auuacgccuu ggaauuucga ugaggugguc     1440

gacaaagaaa aguccgccga gaaguuuauc acccggauga cacugaacga ucuguaccuu     1500

cccgaagaga aaguucuccc uaagcacagc cauguguaug aaaccuucac aguguacaau     1560

gagcugacca aaauuaagua cgucaacgag cagggagaau cuuucuuuuu cgacgcuaau     1620

augaaacagg agaucuuuga ucacguguuc aaggaaaaca gaaagguuac gaaagccaag     1680

cugcucagcu aucugaauaa cgaguuugaa gaguuccgca uuaaugaccu uaucggccug     1740

gacaaagauu ccaagucuuu uaacgcgagc cucgggacau accaugaccu gaaaaagauu     1800

cuggauaagu ccuuccuuga cgauaaaacc aacgagcaaa ucaucgaaga cauugugcuc     1860

acccugacac uguuugagga ucgggacaug auccacgaaa gacuccagaa guauagcgau     1920

uucuuuaccu cucagcagcu gaaaaagcuc gagcgccggc acuacacagg auggggcaga     1980

cuguccuaua aacucauuaa ugggauccgc aacaaggaaa auaacaagac gauucuggac     2040

uuccugaucg augacggaca ugcuaaucgg aacuuuaugc aacuuaucaa ugaugagagc     2100

cucucuuuca aaaccauuau ccaggaggcc caggucgugg gcgacguuga cgauauugaa     2160

gccguggucc acgaccugcc cggcuccccg gcuaucaaga aagggauucu gcagagcgug     2220

aagaucgucg augagcucgu gaaaguuaug ggagacaacc cugauaauau cgugauugaa     2280

auggccagag agaaccaaac aaccaaucgg gagcgcuccc agucucagca gagacugaag     2340

aaacuucaaa acagccugaa ggaacucggc uccaauaucc ugaacgagga aaagcccucu     2400

uacauugagg acaaagucga aaauagccau cugcagaacg aucagcuuuu ccucuauuac     2460

auccagaacg ggaaggacau guacacagga gaugagcugg acauugauca ccuguccgac     2520

uaugacaucg aucacaucau uccucaggcu uuuaucaaag acaauagcau ugauaaccgg     2580

gugcucaccu cuuccgccaa gaauagaggc aaaagcgacg auguucccuc ucuggacauc     2640

gugcgcgcgc ggaaggccga gugggucaga cuuuacaagu ccgggcugau uagcaaacgc     2700

aaguucgaua accucacgaa agcugaaaga ggcggacuga ccgaggccga caaggcuggc     2760

uuuaucaaac ggcaacuggu ggaaacacgc cagauuacca agcauguggc ccaaauccuu     2820

gaugcgagau ucaauacaaa gcgggacgag aacgauaaag uuauccgcga cgucaaggug     2880

auuacccuga aaucuaaucu cgugagccag uuuagaaagg aauuuaaauu uuauaagguc     2940

cgggagauca acgauuacca ccaugcccac gacgcuuauc ugaaugccgu gguugggacg     3000

gcucuccuga agaaauaccc caaacuuaca ccugaguucg uguauggaga auacaaaaag     3060

uacgacgucc gcaaacugau ugccaagucc agcgaugacu auucugagau gggcaaggcc     3120

accgcuaaau acuuuuucua uuccaaccuc augaacuuuu ucaagacaga agugaaauac     3180

gcggauggga gaguuuucga gcggccggac aucgaaacca augccgaugg agaggugguc     3240

uggaauaagc agaaagacuu ugauauugug cgcaagguuc ugagcuaucc ccaggugaac     3300

aucgucaaaa agguggagga acaaaccgug ggccagaaug ggggccuguu cgacaacaac     3360

auccuugcua agccugagaa agaugacaag aaaaagcugu uucccauuaa aaagucccuc     3420

ucuacaacga gcuacggagg cuaugccaga gccaccaucg cuuacuccau ucugaucguc     3480

ucugaguucg ggaagaauaa agaacucaag cugcuuggac ugccucuccu ggauaaaguu     3540

aaguacgaga aaaaccccau ugccuaucug aauagcaucg gcaucauuaa cgugaagucc     3600

aucuuuaaac uuccgaaaua cugccuguuc gaauuucagg agaagaauac aaacuaucgg     3660

agauacaugg ugucuaauca ggaacucaaa cgcgcgaacc aacuguuccu caccuguaag     3720

gagacagaac uggucuauaa auacauuaag agcgacaaug auaacaccca ucuugugggg     3780

aaugagaaga cgauccuguc cauuuggaac aaacugguua ucuuuaugga gcggacaaag     3840

cucauugaca aaaagcuguu cgcuagccuu aaacagcuga aggacgaagu ggaucucauc     3900

aauauccugg acaugaccgc caaaucucuc gauuuuacca agauuuccgc cggacagaag     3960

aaaaucacaa uuagcgacuc ucugucccuu ccuaucaaga gauggcaguu cagcucugau     4020

gagaaaaugc ugaagcaacu gaaaaccgcu acacucauuc accaguccgu cacgggccug     4080

uaugaaaccc gcaucgaccu uagcaagcug ggggaggaau ga                        4122


<210>  4321
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4321
auggagaaga ccuacagcau cggccuggac auugggacaa acuccguggg auggucuguc       60

aucacggaug acuauaaagu ucccgccaag aaaaugaagg ugcucggcaa uaccgauaaa      120

aaguacauua agaaaaaccu gcuuggggcu cuccuguucg acagcggaga aacagccgag      180

gugaccagac ugaagcggac agcgcgcaga cgguauaccc gccggaaaaa uagacuucgc      240

uaccugcagg aaaucuuugc uaaggagaug gccaaagucg augaauccuu cuuucaaaga      300

cucgaggagu cuuuccugac agacgaugac aagaccuuug auagccaccc uauuuucggc      360

aacaaggccg aagaggacgc uuaucaucag gaauuuccga cgaucuacca ucugcggaaa      420

caccucgccg auuccucuga gaaggcugac cugcgccuug uguaucuggc ccucgcgcac      480

augaucaaau ucagagggca uuuucugauu gagggagaac uuaaugccga gaacaccgau      540

auccagaagc uguucaauga cuuuguugaa acauacgaca aaauugcuga ugagagccac      600

cucuccgaag uggacgccag cucuauccug accaacaaga uuuccaaaag ccggcgccug      660

gagaaucuca ucaagcagua ccccacagag aagaaaaaca cccuguucgg caaucuuguc      720

gcucuggugc ucggccugca accuaacuuu aagacgaauu ucaaacuuuc ugaagaugcc      780

aagcugcagu ucuccaaaga cacauaugau gaggaccucg aagaacugcu ggggaagauu      840

ggagaugacu acgcggaucu cuuuaccgcc gcuaagaacc uguaugacgc cauccuucug      900

agcggcaucc ucacaguuga ugacaauucu accaaagcuc cccuguccgc cagcaugauu      960

aagagauacg aagaacacca ugaggaccuu gaaaaacuga agaaauucau caaggugaac     1020

aacuuugaua aguaucacga gauuuucaaa gacaagucca aaaaugggua cgccggauau     1080

aucgaaaacg gcgucaagca ggauauuuuc uacaaacauc ucaagucuau caucagcgag     1140

aagaaugggg gacaguacuu ccuggacaaa auugaacggg aggauuuucu gcgcaagcaa     1200

agaaccuucg acaacggcuc caucccucac cagauucacc uccaggagau gcgggcuauc     1260

cugagacgcc agggcgaaua uuaccccuuu cuuaaagaga aucaaagcaa gauugagaaa     1320

auccugacau uccggauccc uuauuacgug gggccgcucg cgagaaagaa cucucgcuuu     1380

gccugggcua aauaucauuc cgaugagccc auuacgccuu ggaauuucga cgaggugguc     1440

gauaaggaaa agagcgccga gaaauuuauc acccggauga cacugaacga ccuguaccuu     1500

cccgaagaga agguucuccc uaaacacucu cauguguaug aaaccuacgc cguguacaau     1560

gagcugacaa agauuaaaua ugucaacgag cagggaaagg agagcuucuu cgauuccaau     1620

augaagcagg agaucuuuga ccacguguuc aaagaaaaca gaaagguuac caaagagaag     1680

cugcucaauu accugaacaa agaauuuccc gaguaucgca uuaaggaucu uaucggccug     1740

gacaaggaga auaaaucuuu caacgcuagc cucgggacgu accaugaccu gaagaaaauu     1800

cuggauaagu ccuuucuuga cgauaaagug aacgaagagg ucaucgaaga caucauuaag     1860

acacucaccc uguucgagga uaaggacaug auccacgaac ggcugcaaaa auauagcgau     1920

auuuuuaccg ccaaucagcu caagaaacug gagagacgcc acuacacagg auggggccgg     1980

cuuucuuaua agcugaucaa cgggauuaga aauaaagaga acaauaagac cauccucgac     2040

uaccugaucg augacggauc cgcgaaccgc aauuucaugc agcugauuaa cgaugacaca     2100

cuuccguuua aacagaucau ucaaaagagc caggugguug gcgaugugga cgacaucgaa     2160

gcugucgugc augaucuccc uggcucuccc gccauuaaga aagggauccu gcaguccguc     2220

aagaucgugg acgagcuggu uaaagugaug ggaggcaauc cugauaacau ugucaucgaa     2280

auggcccggg agaaucagac gaccaacaga gggcggagcc aaucccagca gcgccucaag     2340

aaacugcagu cuagccuuaa ggagcuggga uccaauauua ucaacgaaga gaagcccucu     2400

uacauugaag acaaagugga gaauagccac cuccaaaacg aucagcuguu ccuguauuac     2460

auccagaacg gcaaggacau guauacaggg gaugaacuug acaucgauca ucucuccgac     2520

uacgauauug accacaucau uccccaggcu uuuaucaaag acgauagcau ugacaauaga     2580

guucugaccu cuuccgccaa gaaccggggc aaaagcgaug acgugccuuc ucuggauauc     2640

gucagagcuc gcaaggccga gugggugcgg cucuauaagu ccggacugau uagcaaaaga     2700

aaguucgaca aucuuacaaa agcggagcgc ggcgggcuga ccgaagccga uaaggcugga     2760

uuuaucaaaa gacaacucgu ggagacgcgg cagaucacca agcacguugc ccagauucug     2820

gacgcucgcu ucaacacaga acaugaugag aaugacaagg ucaucagaga ugugaaagug     2880

auuacccuga agucuaaccu ugucagccaa uuccggaaag acuuugaauu uuacaaggug     2940

cgcgagauca augauuauca ccaugcccac gacgcguacc ugaacgccgu ugugggcaca     3000

gcucuccuga aaaaguaccc gaagcucgcc cccgaguuug ucuaugggga auacaaaaag     3060

uaugacguga gaaaacugau ugcuaagucc agcgaugacc auucugagau gggaaaagcc     3120

accgccaagu acuucuuuua uuccaaucuu augaacuucu uuaaaacgaa gguuaaguac     3180

gcugauggca gcguguauga acggccuauc aucgagacaa augcggacgg ggaagucgug     3240

uggaacaaac agaaggauuu cgagauuauc cgcaaaguuc uguccuaccc ccaggugaau     3300

auugucaaga aaguggagga acagaccgug ggccaaaacg gaggccucuu ugacaauaac     3360

auccuggcca agccugagaa ggaugacaaa aagaaacugu uccccauuaa gaaaucucuu     3420

agcacaaccu ccuacggggg auaugcuaga gccaccaucg ccuacucuau ccugauuguc     3480

agcgaauuug gcaagaacaa ggagcucaaa cugcucgggc ugccgcuucu ggauaagguu     3540

aaauaugaga agaauccuau cgcuuaccuc aacuccauug gaaucauuaa ugugaaaucu     3600

aucuucaagc ugcccaagua uugccuguuc gaauuucagg agaaaaacac aaauuaccgg     3660

cgcuauaugg ugagcaacca ggaacuuaag agagccaauc agcuguuccu cacguguaaa     3720

gagaccgaac uggucuacaa guacauuaaa uccgacaacg auaauacaca ccucgugggc     3780

aacgagaaga ccauccugag caucuggaau aaacuuguua uuuuuaugga gcggacaaag     3840

cugaucgaca agaaacuguu cgcgucucuc aagcaacuga aagaugaagu gaaccuuauu     3900

aauauccugg acauugcuuc caagagcauc gacuuuacca aaaucucugc cgggcagaag     3960

aagauugucc ucaacgauuc ccugagccuc ccuaucaaaa gauggcaguu caagucugac     4020

gagaaaaugc ugaagcagcu uaaugaugcc acgcugauuc accaauccau cacaggccug     4080

uaugaaaccc gcauugaccu cagcaaacug ggagaggaau ga                        4122


<210>  4322
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4322
augaaccuga gacgggcccg cgacuacagc aucggccucg aucuggggac cggauccgug       60

ggcugggcug ucacagacgc cgaggggaau cuuuauucuu ucaagggaag acccgcgaug      120

ggcagccgga cguuuccuaa cgcugaaccg gccuccguug cccgcauucc cagagggcag      180

cggcgcagau acgagcggcg cagacaacgg aucaaacugc uccagcaguu cuuuaugucu      240

gauauggaac gcguggaccc ugauuucuuu accagacuga aucagagcac acuucuggcu      300

gaggacaagg augaacggau ugccuccugc gcuuggccca ucuucaacuc uagcgacuuu      360

accgagaaag auuauuacaa gaaauucccu acaauuuauc accuccgcgc cuuucugugu      420

gaguccgacg aaaagggaga ucugagacuc guguaccugg cgcuucauaa uaucgucaag      480

caccggggca acuuccugua ucaagacaau cccgcccuca gcgcuucuga ugccuccgug      540

gacgaggcug uuagacagcu gguggacgcc cuugcggaau ggugccagaa caaagagauc      600

gagcugagcu gugaugccgg ccgcauuauc gaagucuuug gggacgcuuc ucuccggaga      660

accgauaagc gcgaccagcu gaaaggacug cucggccugc cugccgaucu ugcuaagaca      720

auggggaaag agcuggccuc cgccgugcuc ggauacgcug cgaauuucag caagauuuuu      780

cugaucucug acgccgaaca uaaguucucc cuuggcaacg augagaaagu ugaagaguuc      840

gagaccauuu gcccgaauga auguacgagc cuguuuuccg cucuccggac cgccuacucu      900

gccuaucugc ugagcgggau ccucagagac gcuaacggaa agacacuguc cuucugcaaa      960

aagaaagagu acgaucgcua ucacgacgau cuugcccugc ucaaggcgcu ggugcgggaa     1020

uacgcucccg agagcuauca aucuuucuuc ggcggcgaaa gacuugucgu gaaugggcgc     1080

augaccaaac gguacgaccc uacaaaggcc aagggauaua ccaaauacga ccuggugcau     1140

uccgccggca gcuacgauga gcucgcuaag gacgucaaaa agcugcuggg gucucucgcc     1200

gcugaugaca gcagauauca gcgcaugaug caggccuuug augaggaacg guuccugaga     1260

cggcuuaaaa cguccgacaa cggaucuauu cccuaccagc ugcaccucga ggaacugcgc     1320

gcgaucaucg ccagacaagg cgaguuuuau ccuuuccuga aggaagagag caagaaaauu     1380

gagucccuug uuagcuuucg gauccccuac uauguggggc cgcucacaac caagaaugcu     1440

gccguggaug uccacggaaa caaaagauuc gcuuggucug aacgcaagcc uggcauggag     1500

uccgccaaag ugcggcccug gaacugggac gauguuauug acagaggcaa gagcgcggaa     1560

aauuuuaucg ugcgcaugac agggaccugu accuaccugc agaacgagaa gguccugccu     1620

aaaugcucuc uccuguauga ggaauuuugu gugcuuaaug agcugaacgg agcccauugg     1680

uccgcugaug gcgaccacga augccccuuu gauacaagcg acagacgggg gauuguucag     1740

gaccucuucu guggaaagcg cagagugucc uacaaacaag ucgaggauug gcugcggcag     1800

cauggcucuc gcagagcccg ggugagcggg ggccagggag cuuccggcuu cgaaucuaag     1860

augagcuccu acaucuuuuu cagcaaagac guccugcacg uggaucgcau cgaccccgcc     1920

gauuuuccua ugauugagga gaucauucuu uggaauacgc uuuucgaaga cagagauauc     1980

cugaaggagc ggauugaaga ggccuauggg gaccgccuga acgcugaaca gaucaagacc     2040

aucugcaaaa agagauuuac aggauggggc cggcucucua gacgccugcu uaccgggauu     2100

aaagcgcaaa cagaucaggg agaccugucc aucauggaua uucuccggga gggcgacccg     2160

aauaacgauc acagagggaa ggccaugguu uucaugggca uccugcauag cgaggaccug     2220

ggauuucaga aaaagauuga ccagaucaau caagaaaaag ugucugcugg caaggucucc     2280

cuugaggauc uccccgggag cccugcccug cgccggucua uuaaccaggc caugagagug     2340

guugacgaaa ucgugagcau cgcuaagugu cccccuuccc acauuuaccu ggaggucacc     2400

cgcgaugaag acggagcccg gaaaggcaga cgcagcacgc ggagauauga ugcgaucaag     2460

gcugcccucg agaaauucaa ggaggaaucu cccacauggu uugaccagga uguguucggg     2520

gaguuucggc gcuccagagc cgaagaccug gaugagcggc uuagccugua cuucaugcag     2580

ggaggcaaau cccucuauuc ugcuaccccg cugaagcugg agcuucucca uaccaaucaa     2640

uaccaggugg accacauuau cccucagagc uauauuaagg augacuccuu ugaaaacaaa     2700

gcccugguuc uggcggggga gaaucagcgc aagucugacg cucuccugau cgauagcucc     2760

aucagaacag ccauggcccc cuacuggcgg gcucuccauc aagccggccu gaugggagac     2820

aaaaaguuca acucucucau gugcagcucc auuagcgaua aaagaguccg cggcuucauc     2880

gcucggcagc ugguggaaac cucucagauu gugaagcugg ccgggaugau gcuucaaucc     2940

aauuacccug aggcggacgu ccagcccgug aaggccagcc ugggacacga ucucagaguu     3000

caggcuggcu uuaucaaaug ccgcgaagug aacgacuucc accaugccca cgaugcucug     3060

auugccugug aggucgggcg guauaucaac cagugccauc cuggaaucua cgacaauccc     3120

auuggcuaug ccagagcugu gcgcgagcuc guucggaagc aaaacgaaga gaugagaaaa     3180

uacgggcgcc ugccgggcuc ugcgggauuu aucgugggcg gguucaugag aacacacguc     3240

gauguggaca cgggagaagu ugugugggau gccgaggagg aacuuucccg gauucgcgcu     3300

uaucuggacu acagcaaugu cuuugugacc agaaugucug aggaaacauc cggcgcccuc     3360

uggaacgcca ccaucuauag cccucacuac gggaagacag cucccacccu gcauauuaaa     3420

cagggacugg augugcagcg guacggcggc uauuccagcg ccacguucgc guacuuuacc     3480

cuuuaugucg cugagaaggg gaagaaacgc aaggccgaac ugcucggaau ccccaucucu     3540

guugccagaa aaauugaggg cgaaccuagc aagcuggacg cuuucguggc ccaggacgcg     3600

gaggcuaaaa agcucaaaca cccgcugauc auucgggcca gaauccuuaa guacgcccgc     3660

uuugagaugg aagggaauca auucuauuuu gcugcccagg augcugugua cuccgcccgg     3720

cagcuggucc ucaagggaga ccuguauucu guggcggcca aggcuuguga uaaacaucug     3780

aacgacagcu gcauuuccaa ugaggaucuu aucagacugu acgacgaacu cguucacaaa     3840

ggcgaucugc ucuguaacaa guucgggaau gugacagacg uccugcgcgc cggacgggag     3900

agauacgcug gccuuucuau uagcgagaaa cggcagcugg ugugcaacgu ucuggccuau     3960

uaccgcuccg ccaccgcuag aguggauauc ucucucgucg gcgggcccaa gcaugcggga     4020

aaucugggca ucgccuucgu gcuuaacaau ccugaagcua uuguuuuugu ggaccaaagc     4080

gucacaggga uguucgagaa gcggacccgc guggaacugu ga                        4122


<210>  4323
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4323
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaua acgaaagaga gaaguccucu aucauuaaaa ucggcguccg gguuaauccc      120

cugacagugg acgaacagca aaacuucgag aaggggaaaa gcauuacgac caaugccgau      180

cgcacacuca agagauccau gcggcgcaac cugcagagau uuaaacuucg gaaagaaaau      240

cugaucgaga uccucaagaa acagaaguuc auuaacgacg agaccauccu gucugaaaau      300

ggaaacaaaa gcacauuuga gaccuacaau cugcgcgcga aguccgcuac agaagagauu      360

acccucgagc aguucgccag agugcugcuu augaucaaca agaaacgggg cuauaagucu      420

agccgcaaag ccaaguccca agaugaaggg cagcugauug acggaaugga gaucgcuaaa      480

aagcucuaca augauaaccu gacgccuggc caguuugucu uccagauucu uaaagaaggc      540

aagaaguaua ucccggacuu uuacagaagc gaucugcaag ccgaguucga caaaaucugg      600

ucuuuucaga agcaguucaa ucccgauauu cucaccgaag aguuuaacaa acagaucauu      660

gggaagggaa aagugaacac aaccaagauc uuccuggcua aguauggcau uuacacaauc      720

gacaauaaag gggccgauaa gaaaucccaa gcguauaacu ggcggguuga ggcccugacc      780

aaucagcuca cgaaggagga acuggcuuuu gugaucagcg accuuaacgg aauuaucucu      840

aauuccagcg gcuaccuggg gucuauuucc gaccgcagca aagaacucua cuucaacaag      900

cagacagucg gacaauaucu gaucgcccag cuuaaggaga auccucacac ccagcugaaa      960

aaccaggugu ucuacagaca agauuaucuc gacgaauuua auacaauuug ggagacccag     1020

gcuaaguucc auaaagagcu gaccaacgaa cugaaggagg aaauccggga uaucauuauc     1080

uuuuaccagc gcaaacucaa gucccagaag ggccugauuu cuuucugcga guuugaaagc     1140

aaacaaaucg agauuaauau cgacggcaag aaaaagcuga aaacaguugg guccaaggug     1200

ugucccaaaa gcucuccucu uuuccagguc uuuaagauuu ggcagauccu caacaauauc     1260

caggugauug agaaguccac gggaaaaagc aaguuccugg aacaagauga gaaagaaauc     1320

cuuuuuaagg agcugaacuu caaagacaag auuucuaaga ccgaugcccu caaaauccug     1380

uauaagaauu acaaagagcu ggacuuuaac uucaaggaaa acauugaggg caauagaaca     1440

caggcgaccu uucucaaagc cuuccagagc aucaucgaac uguccgggca cgaggugucu     1500

gauuucagca agauuaagac agacgaaauc cuugagauug ucacccccau cuuugaggga     1560

uucggcuuua acacgaaucu gcuccaguuc aacuccaauc ugaaagggaa ggauuuugaa     1620

aaacaagcug uuuaucagcu guggcaucuu cucuacagcu ucgagggaga caacucuaau     1680

acaggcaacg aaaagcugau ucagaaaauc uccgagcugu acggcuuuga acgggaguau     1740

gccaucauua ucgcuaauau uagcuuccaa ccugacuacg ggucucucuc caccaaggcc     1800

aucagaaaga uucugccgca ccuuaaagag ggaaucgaau uuagccuggc cugcgaguau     1860

gcuggcuacc gccauuccaa gaacucucuc accaaagaag agaagaauaa aaagauccug     1920

aaagauaaga uugacauccu gcccaagaac agccuucgga auccuguggu ggaaaagauu     1980

cucaaccaga ugaucaaugu cgugaacgcg acaauugaga ccuaugggaa gcccgaugag     2040

aucagaauug aacuggcccg cgagcugaaa aaguccgcua aagaacggga ggaccucaca     2100

aacgccauca auaagucuac gcuggaucac gagaaguaca aagcccuucu gcagaaggag     2160

uucggacuca gcaacguuuc cagaaaugac aucauucgcu auaaacugua ccuggaacuu     2220

gaacacaacg gcuuucauac ccucuacagc aauacauaua ucgagccuaa caagcuguuc     2280

ucuaaagaau uugauauuga gcacaucauu ccccaguccc ggcuguucga cgauagcuuc     2340

ucuaauaaga ccaucgaguc cagaagcgcu aacaucgaaa aggggaauga gacagccuuu     2400

gacuacgugu cuaacaaaaa uggcgaaguc ggacucaagg auuaucugaa aaacauugag     2460

gaccuuuuca auaacggcaa gaucagcaaa accaaguaca auaagcugaa aaugaagggg     2520

gaugaaauuc cguccgacuu uaucgcgcgc gaccuccggg auacgcaaua uauuagcaaa     2580

aaggcuaaau cuauccugga ggaccuggug aaggauguug uguccaccgg aggcagcauc     2640

acagacagac uucgcgagga uuggcagcuc auugacauca ugaaggaacu gaacugggau     2700

aaauacgaca agcuggggcu caccgagguc auugaaaaca augauggaca uaaaaucaac     2760

cggauuaagg acuggacaaa aagaaaugau caccggcacc augccaugga cgcccugacc     2820

aucgcuuuca cgaagcgcuc cuauauucag uaccuuaaca aucugaacgc cagaucugau     2880

aaaagcggcu ccaucuacgg gaucgagcag aaggaacucu aucgggacaa gaauaacaaa     2940

cugagauuua agccucccau uccucuggac gauuuccgcg cugaggccaa aaagcaacuu     3000

gagaauacac uggugucuau caaagcgaag aacaaggucg ccaccauuaa uaucaacaau     3060

accaaaaagc agggcggaga aaacaaaaag aaacagcuca caccccgggg ccagcugcac     3120

cucgagacca uuuacgggag cauccaacag uaugugacaa aggaagagaa gguuggagcu     3180

aauuuuaaca aagagcugau cgaaaaggug gccaauaaaa aguguagaga ggcccuucug     3240

aaacgccuca uugaguucga caacgauccc aagaaggcuu uuacgcggaa aaacucccug     3300

aauaagaacc cucuguacau cgacaccauu aaaacauaua aggucccgga aaaagugaag     3360

accguuacaa ccgaaacgau cuacacaauu agaaaagaua ucaaucccga ccuuaagauc     3420

gauaaggugg ucgacaaaaa gauuaaaaac auccuggagc uccgccugaa ggaguucaau     3480

ggcgaugcga aaaaggccuu uucuaaccuc gacgaaaagc cuauuuggcu gaauaaagag     3540

aaggggauca gcauuaaaag agugaccauc accggaauuu ccaacgcuau cgcccuucau     3600

gauaagaaag acaaggaagg caauuucauc cuggacaacc aggggaagaa acaagcugug     3660

gauuucguua auacaggcaa caaucaccau gucgccauuu auaaggacga gaaaggaaac     3720

cuccaggaaa augugaucac cuuuuacgag gccguggagc gggucaacca gggcauuccc     3780

aucauugaua agacauacaa acagaacgaa ggguggcaau uccuguuuag caugaagcag     3840

aaugaguauu ucguguuucc uaacgaaaag acgggauuca auucuaacga gaucgaccug     3900

cuuaaucccg aaaacuacca ccugaucucc ccgaaucucu uucgcguuca gaaaauugcu     3960

accaaggauu auuucuuuag acaccaucug gagacaaacg uggagaauau caccacaacc     4020

aaagaccuca cguggaagcg gcugggcauu aacgggauca uuaauauccu uaaaguccgc     4080

aucaaccaca uuggacagau cgugagcguu ggcgaauacu ga                        4122


<210>  4324
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4324
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucaucacgg augacuauaa gguucccgcu aaaaagauga aagugcucgg caacaccgau      120

aagaaguaca uuaaaaagaa ucugcuuggg gcccuccugu ucgacucugg agagacagcg      180

gaagcuacca gacugaaacg gacagcccgc agacgguaua cccgcagaaa gaaccggcuu      240

cgcuaccugc aggagaucuu ugccgaagag augacaaaag uggaugaauc uuucuuucaa      300

agacucgacg aguccuuccu gcggugggau gacgauaaua agaagcuggg ccgcuauccu      360

auuuuuggga acaaagcuga cgucgugaag uaccaccagg aguucccgac caucuaucau      420

cucagaaaac accuggccga uucuagcgaa aaggcugacc uucggcuggu uuaccucgcc      480

cuggcgcaua ugaucaaauu ucgcggacac uuccuuauug agggcgaacu gaaugccgag      540

aacacggaug ugcagaagau cuuugcugac uucgucggcg uguacgacag aaccuuugau      600

gacucccauc ucucugagau uacaguugau gccgcuagca uccugaccga aaagauuucc      660

aagagccggc gccuggagaa ucucaucaag uauuacccca cagaaaagaa gaacacccug      720

uucgggaauc uuauugcccu ggcgcucgga cugcagccua acuuuaaaau gaauuucaaa      780

cuuucugagg acgccaaacu gcaauucucc aaggauagcu augaagagga ccucggcgag      840

cugcugggga agaucggaga ugacuacgcu gaucucuuua cgucugccaa aaaccuguau      900

gacgcuaucc uucuguccgg cauucucauc guggaugaca auagcacaaa ggcuccccug      960

ucugccucca ugauuaaaag auacgucgaa caccaggagg accuugaaaa gcugaaagag     1020

uucaucaagg ccaacaagag cgaacucuau cacgauauuu uuaaagacaa gaacaaaaau     1080

ggguacgcgg gauacaucga gaacggcgug aagcaggaug aguucuauaa auaccugaag     1140

aauacccugu ccaagaucgc cggcucugac uauuuucucg auaaaauuga acgggaggac     1200

uuccugagaa agcagcgcac auuugauaac gggagcaucc cucaucaaau ucaccuucag     1260

gaaaugcaug cuauccugcg gagacaggga gacuacuauc ccuuccucaa agagaaucag     1320

gaucgcauug aaaagauccu gaccuuucgg aucccuuacu augugggccc gcuggccaga     1380

aaagacuccc gcuucagcug ggccgaguac cacucugaug agaagauuac ccccuggaac     1440

uuugacaaag ucaucgauaa ggaaaagucc gcugagaaau ucauuacacg gaugacgcuu     1500

aaugaccucu accugccuga agagaagguu cugcccaaac acagccaugu guaugagacc     1560

uacgccgugu auaacgaacu cacaaagauc aaauacguca augagcaagg gaaggacagc     1620

uuuuucgaua gcaacaugaa gcaggaaauu uucgaccacg uguuuaaaga gaauagaaag     1680

guuaccaaag aaaagcugcu uaacuaucug aauaaagagu ucccugagua ccgcaucaag     1740

gaucucauug gacuggacaa ggaaaacaaa uccuuuaaug cgucucuggg cacauaucau     1800

gaucuuaaga aaauccucga caaggcuuuc cuggaugaca aagugaacga ggaagucauc     1860

gaggauauua ucaagacccu gacgcucuuu gaagacaagg auaugauuca cgagcggcug     1920

cagaaauaca gcgacaucuu cacagccgau caacuuaaga aacuggagag acgccauuac     1980

accggguggg gacggcucuc cuauaagcug auuaacggca ucagaaauaa agaaaacaau     2040

aagaccaucc uggacuaccu uauugacgau ggcagcgcca accggaauuu uaugcagcuc     2100

aucaacgacg auacacugcc cuucaaacag auuauccaga agucucaagu gguuggggac     2160

guggaugaca uugaggcugu cgugcacgau cugccgggau ccccugccau caagaaaggc     2220

auccuccaga gcgucaagau uguggacgaa cugguuaaag ugauggggga uaaucccgac     2280

aacaucguca uugagauggc ucgcgaaaau cagaccacaa acagaggacg gucucagucc     2340

caacagagac uuaagaaacu gcagaauagc cucaaggagc ugggcuccaa cauccugaau     2400

gaggaaaagc cuucuuauau ugaggauaaa guggaaaaca gccaccuuca gaaugaccaa     2460

cucuuucugu acuauaucca gaacgggaag gacauguaca cgggcgauga gcuggacauc     2520

gaucaucucu ccgacuauga uauugaccac aucauucccc aggccuucau caaagaugac     2580

ucuauugaua accgcguucu gaccagcucc gcgaagaauc ggggaaaaag cgacgaugug     2640

cccucucuug acaucgucag agcccgcaag gcugaguggg ugagacugua caaguccggc     2700

cucauuagca aacggaaguu ugauaaccug acaaaagccg aacgcggggg acugaccgag     2760

gcugacaagg ccggcuucau caaaagacag cuuguggaaa cacggcaaau caccaagcau     2820

guugcgcaga uucuggacgc ccgcuuuaau acggagucug augaaaacga caaggucauc     2880

agagauguga aagugauuac ccucaagucc aaucugguca gccaguuccg gaaagacuuc     2940

gaguuuuaca aggugcgcga gaucaacgau uaucaccacg cucaugacgc cuaccucaau     3000

gcuguugugg ggacagcccu gcuuaaaaag uauccuaagc uggccucuga guucgucuac     3060

ggagaauaua aaaaguacga uauuagaaaa uuuaucacca acagcuccga caaggcuaca     3120

gcgaaauauu ucuuuuacag caaucucaug aacuucuuua agcgggugau ccgcuacucu     3180

aauggcaaag uuauugugag accggucgug gaauauucca aggauaccga ggacaucgcc     3240

ugggauaaga aaagcaacuu ccggacgauu ugcaagguuc uguccuaccc ccaagugaau     3300

aucgucaaaa agguggaaac acagaccggg ggcuuuucua aagagagcau ucugccuaag     3360

ggagacuccg auaaacuuau ccccagaaaa acaaagaagg cuuauuggga caccaagaaa     3420

uacggcgggu ucgacucucc uaccguggcc uauagcgucu uuguuguggc cgauguggag     3480

aagggaaagg cuaaaaagcu gaaaacaguc aaggaacucg ugggcaucuc cauuauggag     3540

cgcucuuucu ucgaagagaa ccccguugaa uuucuggaga auaaagggua ccacaacauc     3600

cgggaggaca agcucauuaa gcugccgaaa uauagccuuu ucgaauuuga gggaggcaag     3660

agacgccugc ucgccuccgc gagcgaacug cagaaaggga acgagauggu gaucccuggc     3720

caucugguca aacuucugua ccacgcucag cggauuaauu cuuucaacuc cacgaaauac     3780

cucgauuaug ugagcgccca uaagaaagag uuugaaaagg uucugucuug uguggaggac     3840

uucgccaauc ucuacgucga uguggaaaag aaccugucca aaaucagagc uguugccgac     3900

agcauggaua auuuuucuau ugaggaaauc uccaacagcu ucaucaaucu ucugacccug     3960

acagcgcucg gagcucccgc cgacuuuaac uuccugggcg agaagauucc ucgcaaacgg     4020

uauaccucca caaaggagug ccuuaaugcc acccugaucc accaaagcau uacggggcuc     4080

uacgaaacaa gaaucgaucu gucuaaaauu ggagaggaau ga                        4122


<210>  4325
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4325
auggagaaca agaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucaucacag augacuauaa aguucccucu aagaaaauga aggugcucgg caauacggau      120

aaaagauuca uuaagaagaa ccugaucggg gcccuucugu uugacgaagg aaccacagcc      180

gaggcgcggc gccucaaaag aaccgcucgg cgcagauaca cacggcgcaa gaauagacug      240

cgguaucuuc aggaaauuuu cgccgaggaa augagcaaag uggauuccuc uuucuuccac      300

cgccuggacg auagcuuucu caucccugag gacaagaaag gcuccaagua cccgaucuuc      360

gccacccuga uugaggaaaa ggaguaucau aaacaauuuc ccacaaucua ccaccugaga      420

aagcagcucg cugauucuaa agaaaagacc gaccugcggc uuauuuaucu ggcccucgcu      480

cauaugauca aauaccgcgg gcacuuccug uacgaggaua cguuugacau uaagaacaau      540

gauauccaga aaauuuucaa cgaguuuauc agcaucuaua auaacaccuu cgaaggaaau      600

ucccuuagcg gccagaacgu ccaaguggag gccauuuuua cagacaagau cucuaagucc      660

gcgaaaagag aacggguucu gaaacucuuc ccugacgaga aaagcaccgg ccuguucucu      720

gaauuucuga agcucauugu ggggaaucag gccgauuuca aaaagcauuu ugaccuggag      780

gagaaggcuc cccuucaguu cucccgcgau acauacgacg aagaucugga gaaccuccug      840

ggacagaucg gcgacgauuu ugccgaccuu uucgucgcug ccaaaaagcu guaugaugcg      900

auucuccuga gcgggauccu gaccgugacg gacccuucua caaaagcucc ccucuccgcu      960

agcaugaucg aaagauacga gaaucaccaa aaggaucugg ccacccuuaa acaguuuauu     1020

aagacaaacc ugccugaaaa guaugacgag guuuucuccg accagucuaa agauggauac     1080

gcuggcuaua ucgacgggaa gaccacccag gagagcuuuu acaaauacau uaagaaccuc     1140

cuguccaaau ucgaaggagc cgauuauuuu cuugagaaga ucgaacggga ggacuuccug     1200

agaaagcaac gcacauuuga uaauggcagc auuccgcacc agauccaucu ccaggaaaug     1260

aacgccaucc ugcggagaca aggcgagcac uaccccuucc ugaaagagaa uaaggagaaa     1320

auugaaaaga uccucacguu ccgcauuccu uauuacgugg ggccccuggc ucggggaaac     1380

agagacuuug cguggcuuac ccgcaauucu gaucaggcca uccggccuug gaacuucgaa     1440

gagauugucg acaaggcuuc cagcgccgag ucuuuuauca auaagaugac aaacuaugau     1500

cuguaccucc ccgaagagaa agugcugccg aagcauagcc ugcuuuauga aaccuucgcc     1560

guguacaaug agcucacaaa ggucaaauuu auugcugaag gccugagaga cuaccaguuc     1620

cuggauuccc gccagaagaa agacaucuuu uauacccucu ucaaggccga ggauaaacgg     1680

aagguuacgg agaaggacau cauucaauac cugcacacag uggacgggua ugauggaauc     1740

gaacuuaaag gcauugagaa gcaguuuaac gcgucucuga gcaccuacca cgaccuccug     1800

aaaaucauua aggauaaaga auuuauggac gauccuaaua acgaggaaau ccuggagaau     1860

aucgugcaua cccuuacaau uuuugaggac agagaaauga ucaagcagcg ccucgcucag     1920

uaugauuccc uguucgacga gaaggucauu aaagcccuga cccggagaca cuacacaggg     1980

uggggaaagc ucagcucuaa acugaucaac ggcauucggg auaagcaaac gggcaaaacc     2040

auccuugacu aucugaugga ugacggguac aauaaccgca acuuuaugca gcucaucaau     2100

gaugacgaac uguccuucaa ggagauuauc aagaaagccc aggugguugg aaagacagac     2160

gaugugaaac aggucgugca agaacugccc ggcagcccug cuauuaagaa agggauccuu     2220

cagucuauua agcucguuga cgagcuggug aaagucaugg gacaugagcc cgaauccauc     2280

gugaucgaga uggccagaga aaaccagacc acagcucggg gcaagaagaa uagccagcaa     2340

agauacaaac gcauugagga uucccugaag auccucgccu cugggcugaa cgcgaaaauu     2400

cuuaaggagc accccaccga caauauccag cugcagaacg auagacucuu ccuguauuac     2460

cugcaaaaug gccgggacau guauacggga aaaccucuug auauuaacca gcucagcucc     2520

uacgacaucg aucauauugu cccgcaggcc uuuaucaagg acgauucucu ggacaaucgc     2580

gugcugacca gccucaagga caacagaggc aaauccgaua auguucccag ccuggaagug     2640

gucgagaaga ugaaaacauu cuggcagcaa cuucuggacu cuaagcucau cuccuaucgg     2700

aaauuuaaca aucugaccaa ggcugaacgc gggggacugg augagagaga caaggugggc     2760

uucauuaaac ggcagcuugu ugaaacacgc cagaucacca agcacguggc ccagauucug     2820

gaugcuagau acaacacgga ggucaaugag aaagacaaga aaaaccggac agugaagauc     2880

auuacccuca agagcaaccu ggugucuaau uuucgcaaag aguucagacu cuauaagauc     2940

cgggaaauca acgauuacca ccaugcccac gacgcguacc ugaaugccgu ugucgcuaaa     3000

gccauucuua agaaauaucc uaagcuggaa cccgaguuug uguacgggga uuaucaaaag     3060

uacgaccuca aacgcuauau cuccagaagc aaggauccua aagaaauuga gaaggcuaca     3120

gagaaauacu ucuuuuauuc uaaccugcug aauuucuuua aggaagaggu gcauuacgcc     3180

gacggaacca ucgucaaacg ggaaaacauu gaguacagca aggauaccgg cgaaaucgcc     3240

uggaauaagg agaaagacuu cgcuacaauc aagaaagugc uuucccugcc ccagguuaac     3300

auugugaaga aaagagaggu ccagaccggg ggcuucagca aggaaucuau ccucccgaag     3360

ggaaauuccg acaaacugau uccucgcaag acgaaagaua uccucuggga cacaaccaag     3420

uauggcgggu uugauagccc cgugauugcg uacuccaucc ugcuuauugc cgacaucgag     3480

aaaggaaagg cuaagcggcu gaaaacaguu aagacccucg ugggcaucac gauuauggaa     3540

aaagccacau ucgagaaguc uccuaucgcc uuucuggaga acaaagggua ucacaauguc     3600

agaaaggaaa acauucugug ccuucccaag uacagccugu ucgagcucaa aaauggacgc     3660

cggagaaugc uggcuuccgc caaggaacuc cagaaaggca acgagaucgu gcugccuguu     3720

caccuuacca cccugcugua ucaugcgaag aauauucacc gccucgauga acccgagcau     3780

cuggaguaca uccaaaaaca ccggaacgaa uuuaaggggc uucugaaccu cgugucugag     3840

uucagccaga aauauguccu ggcugacgcc aaucucgaaa agaucaagaa ccuguacgcc     3900

gauaaugagc aggcugacau ugaaauccuu gccaacuccu uuauuaaucu gcugacauuc     3960

accgcgcucg gcgcuccggc cgccuuuaaa uucuuuggaa aggaugugga cagaaaacgc     4020

uacacaacgg ugucugagau ccugaacgcu acccuuauuc aucaaagcau cacaggccug     4080

uaugagaccc ggaucgaucu cuccaagcug ggggaagacu ga                        4122


<210>  4326
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4326
auggagacca aguucacaua cuaucugggc cucgacaugg ggacggauag ccugggaugg       60

gccgugaccg acuccucuua ccacauccuu agagcuaaag gcaaggaucu guggggaguc      120

cggcucuuuc ccagcgccga aacauccguu gagcgcagaa ccuaucgggu gaaccgcaga      180

cggcgccaga gagaaguggc gcggauuggg gcucugaaag agcuuuucgc cgacgaaauc      240

gagaaggucg auccuggcuu uuucgugcgc cuggacgagu cuaaauuuca uauggaagau      300

agaagcgaga auaacaagca aaaguacggg cucuuugccg acaaacuguu uuccgaugcu      360

ggauauuaca agaaauaucc gacaauuuuc caucugcgga aggaacuccu ggagucugag      420

gaaccccacg acguucgccu uguguaccug gcccuccuga auauguuuaa acacagaggc      480

cauuuccuua acagcucccu gcagaucgag ggcgaaaaua gcaacauugc ugagucuuac      540

cagauguuug ucgauacccu cacagacgaa gaucuggacu ucccugaaca gguguccggg      600

gagcggcugc aagagaucuu ucgcagcucu gacuccagaa agaagaaagu ugagaaucuc      660

cuggccgaaa ugaaccuuag caagaaacag aagcgggaga uccaguucgu gaaucugauu      720

ugcggacuca aaggcaagcu gucugaacuu uuccccgaua aagaguauga cgaggaacag      780

aagaagaucg cguuuuccuu ccaagauagc gacuacgagg auaaagaaaa caccauucag      840

gagcugcucg acgaauccga gaugaauaug aucuaugccg ucaaggcugu gcacgauucu      900

ggggugcuga gcggaauuau gaacggcgag agauaccugu ccgucgcccg cguugcuagc      960

uaugaaaaac acaagaaaga ccuccagauc cugaaggaua uucuuaagaa auacgacacg     1020

aaggaguauu uugaaauguu ccggaaugag ucugccaaau ccgagagcua cucugcguac     1080

guggggagcg ugaaguauca uggaaccuuu agacgcggcc uguccggcgg gaaacaggaa     1140

gaucucuaca agcacaucaa gaaaguccug ggaaaguucc cucaagacga cgagggcgug     1200

acauauaucc uugaagagau ugaaaccgag ucuuuucugc ccaaacagcu cacagccagc     1260

aacgggguua ucccuaauca ggugcaugcu cgggagaugc uggccauucu gaagcacgcu     1320

gaacucuacc ugccguuccu ucaagagaaa gaugacaccg gacuguccgu cagcgaaaag     1380

auccucaagc uguuuauguu ccagauuccc uauuacgugg gcccucuggg gcaggaguau     1440

aaagauaaga aaggauacaa cguuugggcc gaaagaaagg ccucuggcaa aaucuacccc     1500

uggaacuuug aggacaagau cgaugugaaa aaguccgcug agaaguucau uggccgcaug     1560

guccggagau guacguaucu uagcggggaa caggcgcucc cuaaacaauc ucugcuguac     1620

gagaaguuuc aggugcucaa ugaacugaac aaucuucgca uccggggaga gaaaauuccc     1680

gucucccuga agcaggaaau cuaucacgac cucuucggcg augggaaaaa ggugagcaug     1740

acaagacuga ccaacuaccu gcuucagaau ggaguuauuu ccggcgagga gaaggacucu     1800

ugcaucagcg ggaucgaugg cggauuccau aacucccucu cuagcguggg caaauuuaag     1860

ggggucuucg gagacacagu gcacaccgau gaacaucgca aaaugauuga ggacaucauu     1920

uucuggggca ccguuuucgg ggauggaaag aaacugcuga aggaaaaggu ggaggagguc     1980

uauggcaauc aacucacaga agcccagaaa aagcggaucu uuggguucaa auuuuccagc     2040

uggggcagac ugucuaaggc uuuccuuacg auggagggag ccuccaaaga cgacggcgaa     2100

auuaaggggc ugauccaggc caugugggau accaacugua aucucaugga gcugcugagc     2160

gaccgguuua cauacaagga uaaacuuaac gaaaugguga ccacagcuga gaagccgcuc     2220

ugcgagugga aaauugaaga ccuggaugag auguaucugu cugccccugu gaagcgcaug     2280

guuuggcaga ccaucagagu cguggacgaa gugcaacaga ucauuggaug ucccccuaaa     2340

cggaucuucg ucgagaugac gagauccgau ggcgaaaagg ggaaacgcac agugagccgg     2400

cagaagaagc ucguugaccu guacaaagcg cuuggaaagg agggcaaaac cuggucugag     2460

gaacugagcg auacccccga gucccaguuu agaauuaaga aacucuaccu guauuaccug     2520

caaaugggga agugcaugua uacaggcgaa gcuaucgagc uugaacagcu caugaaugac     2580

aaccuguacg auauugacca caucuauccc cgccauuuca ucaaggauga caauauuagc     2640

aacaaucugg ugcucgucaa aaaggagaaa aacgcccaca agucugacuc cuuuccuauc     2700

gagccggaaa uuagaaacaa aaugaugccc uucuggaagu cccugcggaa gaaaggauuc     2760

cuuaccgagg aaaaguacaa ucgccugaca agaauguccg ccuuuacgga ugaggagaaa     2820

gcugccuuca ucggccggca gcucguggaa accucucaag ggacaaaggc uauuacccag     2880

aucuuugagc aggccuucgc gaacagcgaa aucguuuuua gcaaagccuc uguggucagc     2940

gcuuuccgcc agaaguuuga cuguuauaag gugagaugcg uuaauaacua ccaccaugcc     3000

cacgaugcuu accugaauau uguggccgga aacacauauc gggucaaauu caccuccaau     3060

ccucugaacu uuaucaagga ggcgaaaaag aaucccaacu cucgcgaaaa uagauacaac     3120

auggacaaaa uuuucgagug ggaugugacg cggaauggcg agaccgccug gguggcucaa     3180

acaaaggaac auccugggac cauccagcuu gucaagaaau aucugaacaa gaauagcccc     3240

cucauuacaa aaaugguuua cgagaagcac ggaggcauca cccagaaagc cacgaucugg     3300

uccgcuaaga aagccgaaag cgggaacaca gacgccuaua uuccggugaa gaugaacgau     3360

ccucgccugg cugacgugac caaguacggc ggagucacaa ccaucgcggu ggccggcuau     3420

acccuccugg aguacaaagc ugggggaaag acaguuagau cucuugaagc ccugcccauu     3480

uaccucggcg gguccaaaaa gcugacggag gagcagaugg uggaauauuu uagcaauguc     3540

cugcaacagg agaacaaaga aaaggugguu ucugauguga ucauucggaa gaaaaugauc     3600

ccuuccggaa gccuuauuaa guacaauggc uucuauuacu aucuggccgg caaaaccggg     3660

gacagaauca uccucauuuc ugcuguccag cugugucucc ccauggauac aaugcuguac     3720

aucaagaaaa uugagaaggc cugcuccacc ggaaacuucg aagagaagga caaaaauggc     3780

aaacaggugc uuagcaagca acagaacgag uuuguuuaug aacugaucau ucagaaguac     3840

caacagucca ucuucaaaaa gaaggugggg gcgaucggag acacaauuau cgagaaacgc     3900

gaaaaguuug cugagauuag ccucgaggaa caguguaaag uccugaugca gaucuuccug     3960

aauuuucaau cuggcauugg gguggagcuu aagaucaucg aaggaggcaa aaagagcggc     4020

gugaccuacc ugaacaaaaa ggucaugaau guucaggagc ucaagcugau ugaucagucc     4080

gugacggggg uguucucucg ggaaaucgac cuccugacaa uuuga                     4125


<210>  4327
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4327
augaagaaaa gauacagcua uuccaucggc cuggacauug ggaccaacuc ugugggaugg       60

gccgucauca augaggauua caagguuccc gcuaaaaaga ugacaguguu cggcaacacg      120

gaccggaaaa ccauuaagaa gaaucuccug gggacagugc uuuuugauag cggagaaacc      180

gcccaggcgc gcagacugaa acggacaaac cgcagacggu auacccgcag acgguaccgc      240

cucugccaac ugcagaauau cuucgcuaca gagaugguca agguggacga uaccuuuuuc      300

cagagacuuu ccgaaucuuu cuuuuauuac caggacaaag ccuuugauaa gcacccuauu      360

uucggcaaca gcaaagagga acgggccuau cauaagacgu acccgaccau cuaccaucug      420

cgcaaggacc ucgcugauag agaccaaaaa gccgaucugc ggcugaucua ucucgcucug      480

ucccacauua ucaaguuucg cgggcacuuc cuuauugagg gaaaacuuaa uucugagaac      540

acagacguuc agaagcucuu uaucgcccug gugaccgucu acaaucuucu guucgaagag      600

gaacccauug cgggcgagac augugaugcc aaagcucucc ugaccgccaa gacgagcaaa      660

uccaagagac uggagagccu caucucugaa uuuccuggcc agaagaaaaa cgggcuguuc      720

ggaaaucuuc uggcucucgc ccugggccuu cggcccaacu ucaaguccaa uuuugggcug      780

agcgaggacg cgaaacucca gauuacaaag gacaccuaug aagaggaacu ggauaaccug      840

cucgccgaga ucggagacca uuacgcugau cuguuccuug ccgcuaaaaa ucugucugac      900

gccauccucc uguccgauau ucuuacacug agcgacgaga acaccagagc cccucucucu      960

gcuuccauga ucaagcgcua ugaagaacac caagaagauc uggcgcugcu caagaaacug     1020

gugaaggagc agaugcccga aaaauacugg gagauuuuua gcaacgccaa gaaaaauggc     1080

uaugcugggu acaucgaggg aaagguuucc caggaagacu ucuaccggua uauuaagccu     1140

auccuuucua gacugaaagg cggcgaugag uuucucgcca agaucgaccg cgaugacuuc     1200

cugcggaaac agagaaccuu ugacaacggg agcauuccgc accaaaucca ucugaaggaa     1260

cuucacgcca uucuccgccg gcaggagaaa uacuaucccu uccuggcuga acagaaggag     1320

aagaucgagc aacugcucug cuuucgcauu ccuuacuaug ugggaccccu ggccaaaggc     1380

ggaaauucca gcuucgcgug gcuuaagaga cggucugaug aaccuaucac acccuggaac     1440

uuuaaagacg ucguggauga ggaggcuucc gcccaggccu ucaucgaggg aaugacgaau     1500

uacgacaccu aucugccgga ggaaaaggug cucccuaaac auagcccccu guacgagaug     1560

uuuacagucu acaacgaacu gaccaagguu aaauauauug cugagaauau gacaaagccu     1620

cuuuaccucu cugccgaaca gaaggaggcu aucauugauc accuguucaa acagaccaga     1680

aaggugacgg ugaaagaccu gaaggagaaa uauuucagcc aaaucgaagg ccucgagaac     1740

gucgauguga cagggguuga aggagccuuu aaugcguccc ugggcaccua caacgaccuu     1800

cugaagauua ucaaggauaa agccuuccuc gacgaugagg cuaaugccga aauucuggag     1860

gagaucgugc ugauccuuac ccucuuucag gacgaaaagc ugauugagaa acgccuggcu     1920

aaguaugcca accucuucga aaaaucuguc cugaagaagc ugcggaaaag acacuaccgg     1980

ggcugggggc gccugagcag acagcucauc gauggaauga aggacaaagc guccggcaag     2040

acaauucugg acuuucugaa agccgaugac uucgcuaauc ggaacuuuau ccagcuuauu     2100

aaugauagcu cucucgacuu cgagaagcug aucgaugacg cccaaaagaa agcuaucaag     2160

agagaauccc ugaccgaggc cguggccaac cucgcuggga gccccgcgau uaaaaaggga     2220

auccugcagu cucuuaaagu uguggaugag auugucaagg ugaugggcca uaaccccgac     2280

aauaucguca uugaaauguc ccgcgagaac cagacaacgg cccaggggcu gaaaaaugcu     2340

cggcaaagac ucaagaagau caaagaagug cacaagaaaa ccggcagccg cauccuggag     2400

gauaacuccg agagaauuac aaaucugacc cuucaggaca accggcugua ucucuaccug     2460

cuccaggaug gaaaggacau guacacaggc caggaucugg acaucaauaa ccuuucucaa     2520

uaugacauug aucauaucau uccucagagc uuuaucaaag acaauuccau cgauaaccug     2580

guucucacca cgcagaaggc caaucgcggg aagucugaca acgugccgag cauugaaguc     2640

gugagagaua ugaaagaccg gguuuggcgc agacaacugg ccaauggagc uaucucccgg     2700

cagaaguucg aucaccugac caaagccgag cgcggcgggc uugcggacag cgauaaggcu     2760

agauuucugc ggcgccagcu cguggaaaca agacagauua ccaaacaugu cgcccaacug     2820

cucgacucuc gguucaacuc caagagcaau cagaacaaga aacuggccag aaacgugaag     2880

aucauuacac uuaaaucuaa gaucgugucc gauuuucgca aagacuucgg acuguacaag     2940

cuccgggagg uuaauaacua ucaccacgcu caugacgccu accugaaugc ggucgugggc     3000

accgcucugc uuaagaaaua ucccaagcug gaagccgagu ucguguacgg ggauuauaaa     3060

cacuuugacc ucgucaagcu gauuagcaaa ucugauccua gccucggcaa ggccacggcu     3120

aagguguucu uuuacuccaa caucaugaau uucuuuaaag aggaacugag ccuugccgac     3180

ggaacacuga ugaagagacc cguuaucgag accaacacag aaaccggcga gguggucugg     3240

gauaaaguga aggacuucaa aaccauucgc aagguucugu cuuacccuca ggugaauauc     3300

gucaaaaaga cagaaauuca guccggggcu uuuagcaagg aguccgugcu cucuaaagga     3360

aacagcgaua agcugaucga gcggaaaaag ggcugggacc ccaaaaagua ugggggauuc     3420

gauuccccga auacggccua cucuauuuuu guuguggcga aggucgccaa aagaaaggcu     3480

caaaaacuua agaccgugaa agaaaucgug ggcaucacaa uuauggagca ggccgaauau     3540

gagaaggaca acaucgcuuu ccuggaaaag aaaggguacc aggauauuca ggagaagcuc     3600

cugaucaaac ucccuaagua uagccuguuu gagcuugaaa auggccgccg gagacugcug     3660

gccuccgcca acgaguucca aaaaggaaau gaacucgcuc ugucuggcaa guacaugaag     3720

uuccuuuauc uggcgagccg cuacgacaaa cucuccagca agauugaguc ugagcagcag     3780

aaaaagcugu uugucgaaca acaucuccac uacuucgaug agauccugga caucguugug     3840

aaacacgcca ccuguuauau uaaggcugaa aacaaucuua aaaagaucau uucccuguac     3900

aagaaaaagg aggccuauag caucaacgaa caggcccuga acaugcucaa ucuguuuauu     3960

uucacaucuc uuggggcucc cuccaccuuu guguucuuug acgagacgau cgaucggaaa     4020

agauacacaa ccagcucuga cguccugaac gggauucuca uccagcaguc caucaccggc     4080

cuguaugaga cacggauuga ucucagccgc uucgggggag acuga                     4125


<210>  4328
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4328
augaagaaac agaaguucag cgacuacuau cugggcuuug auaucgggac caacuccgug       60

ggauggugcg ucacagaccu cgauuacaau guucugagau ucaacaaaaa ggacaugugg      120

ggcucucggc uuuuugauga ggccaaaacg gcugccgaac gcagagugca acggaauagc      180

cgcagacggc ugaagcgcag aaaguggcgg cucaaccugc uugaggaaau uuucuccgac      240

gagaucauga aaauugauuc uaacuuuuuc cgcagacuga aggaaagcuc ccucuggcug      300

gaggacaaaa auucuaagga gaaauuuacc cuguucaaug augacaacua uaaggauuac      360

gacuuuuaua agcaguaccc cacaaucuuc caccuccggg augaacugau uaaaaauccu      420

gagaagaaag acauccgccu uaucuaucug gcgcuccaua gcauuuuuaa guccagaggg      480

cacuuccugu uugaaggaca gaaccuuaaa gagaucaaga auuucgagac ccuguacaac      540

aaucucauua gcuuccugga agacaacggc aucaauaaau cuauugauaa ggacaacauc      600

gagaagcugg aaaagauuau cugugauucc gggaagggac ucaaagacaa ggagaaagaa      660

uuuaagggca ucuucaacag cgauaagcag cugguggcua uuuuuaaacu uucugucggc      720

uccagcgugu cucugaauga ccucuucgau acagacgagu acaagaaaga ggaaguugag      780

aaggaaaaga ucuccuuucg ggagcaaauu uaugaagaug acaagccgau cuacuauagc      840

auucuggggg agaagaucga gcuucuggau aucgccaaau ccuucuacga cuuuauggug      900

cucaacaaua uucugucuga cagcaacuau aucuccgaag ccaaggucaa acuguacgag      960

gaacauaaga aagaucucaa gaaucugaag uauauuaucc gcaaauacaa caaggagaau     1020

uacgacaaac uuuucaagga uaaaaacgaa aauaacuauc ccgcuuacau uggacugaau     1080

aaggagaaag acaagaagga ggugguugaa aagagcagac ucaagaucga ugaccugauc     1140

aaagugauua agggcuaucu uccuaaaccc gagcggaucg aagagaagga uaagaccauu     1200

uuuaacgaga uccugaauaa aauugaacuc aagacaaucc ugccuaaaca gagaauuucu     1260

gacaacggga cccugcccua ccagauccac gaggucgaac ucgagaagau ccuggaaaau     1320

caguccaaau auuacgauuu ccuuaacuau gaggagaacg gagugagcac gaaggacaag     1380

cugcucaaaa ccuuuaaguu ccgcauuccu uacuacgugg gcccgcuuaa uucuuaucau     1440

aaagauaaag ggggaaacag cuggaucguc cggaaagaag agggcaagau ucugcccugg     1500

aauuuugaac aaaagguuga caucgagaaa uccgccgaag aguucauuaa gagaaugaca     1560

aacaaaugca ccuaccugaa uggcgaggau gugaucccua aggacucuuu ccucuauagc     1620

gaauacauca uucugaacga gcugaauaaa gugcagguca acgacgaauu ucucaaugag     1680

gaaaacaagc gcaaaaucau ugaugagcug uucaaggaga auaagaaagu guccgaaaag     1740

aaauuuaagg aguaucuucu gguuaaccag aucgcuaauc ggacagugga acucaaaggg     1800

auuaaggaca gcuucaacuc uaauuacguc uccuauauca aguuuaaaga uaucuucgga     1860

gagaagcuga accuggacau uuacaaagag aucagcgaaa agucuauucu uuggaaaugu     1920

cucuacggcg augacaagaa gaucuuugag aaaaagauua aaaacgaaua uggggauauc     1980

cugaauaagg acgagaucaa aaagauuaac uccuucaagu uuaauaccug gggaagacug     2040

agcgaaaaac uccugacggg caucgaguuc auuaaccuug agacagggga augcuacucc     2100

ucugugaugg aggcccugcg ccggaccaau uauaaccuca uggaacugcu gagcuccaag     2160

uuuacacuuc aggagucuau cgauaaugaa aacaaagaga ugaaugaggu uagcuacaga     2220

gaccucauug aagaguccua ugugagcccc ucucugaagc gcgcgauccu gcaaacccuc     2280

aaaauuuacg aagagaucaa gaagaucacc ggacgggucc cuaaaaaggu guucauugaa     2340

auggccagag gcggcgauga guccaugaaa aacaagaaaa uccccgcucg ccaggagcag     2400

cugaagaaac uuuaugacag cugugggaau gauauugcca acuuuucuau cgacauuaag     2460

gaaaugaaaa auucccugag cucuuacgac aacaauagcc uccggcagaa aaagcuguac     2520

cuguauuacc uucaauucgg aaaaugcaug uauacaggca gagagaucga ucucgaccgg     2580

cugcugcaga acaacgauac guacgacauc gaucacauuu auccgcgcuc caaggucauc     2640

aaagacgaua gcuucgacaa ucucgugcug guucuuaaga acgaaaaugc ugagaagucu     2700

aacgaguacc cugugaaaaa ggaaauucag gagaaaauga aguccuuuug gagauuccug     2760

aaagaaaaga auuuuaucag cgaugagaag uauaaacggc ucaccgggaa ggacgauuuc     2820

gaacugagag gauuuauggc ccgccaacug gucaacgugc ggcagacaac caaagagguu     2880

ggcaagauuc uucagcagau cgagcccgaa aucaaaauug uguacuccaa ggcggagauc     2940

gccucuagcu ucagagaaau guuugacuuc auuaaggucc gcgagcugaa ugacacacac     3000

caugcuaaag augccuaccu caacaucgug gcugggaaug uguauaacac caaguuuacg     3060

gaaaaaccuu acagauaucu gcaagagauu aaggagaauu acgacguuaa aaagaucuau     3120

aacuacgaua uuaaaaaugc cugggacaag gaaaacuccc ucgagaucgu caagaaaaau     3180

auggaaaaga acacagugaa uaucacccgg uucauuaaag aggaaaaggg cgagcuguuu     3240

aaccuuaacc ccaucaaaaa gggagagacc ucuaaugaaa uuaucagcau uaagcccaaa     3300

cuguaugaug gcaaggacaa caaacucaau gagaaguacg gguacuauac aucccugaaa     3360

gccgcuuacu ucaucuaugu ggaacacgag aagaagaaca aaaaggucaa aaccuucgag     3420

cgcaucacaa gaauugauuc uacgcugauc aagaaugaga aaaaccuuau uaaguaccug     3480

gugagccaga agaaacuccu gaauccuaag aucauuaaaa agaucuauaa agaacagacc     3540

cucaucauug acuccuaccc guauacauuu accggaguug auagcaacaa gaaaguggaa     3600

cugaagaaua agaaacagcu uuaccuggag aagaaauacg aacaaauccu caagaacgcg     3660

cugaaauucg ucgaggacaa ucagggcgag acagaagaga acuauaaguu uauuuaccug     3720

aagaaacgga auaacaauga aaagaacgag accaucgaug ccgugaaaga acgcuauaac     3780

auugaguuca augagaugua cgacaaguuu cuugaaaaac ugucuuccaa ggauuauaag     3840

aacuacauca auaacaaacu cuauacgaau uuccugaaca gcaaggagaa auuuaagaaa     3900

cucaagcugu gggaaaaguc ucuuauucug agagaguucc ugaaaaucuu uaauaagaac     3960

accuacggga aauacgaaau caaggacucc cagacaaaag agaaacucuu cagcuuuccc     4020

gaggacaccg gacggauucg ccugggccag ucuucccuug ggaauaacaa ggaacugcuc     4080

gaggaaagcg uuacaggccu guucgugaaa aagaucaaac ucuga                     4125


<210>  4329
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4329
augagcaaca agcccuacuc caucggccug gacauuggga ccaauucugu gggaugggcc       60

gucaucacag augacuauaa aguuccuagc aagaaaauga aggugcucgg caacacggau      120

aaacacuuca uuaagaagaa ucugaucggg gcccuucugu uugacgaggg aaccacagcu      180

gaagauagac ggcucaaacg caccgcgaga cggcgcuaca caagacggaa gaaccgccug      240

agauaucuuc aggagauuuu cuuugaagag auguccaaac uggacucugg cuucuuucau      300

cggcucgaug acuucuuucu gaucccggaa gauaagcgcg ggagcaaaua ccccaucuuc      360

gcuacccugg ccgaggagaa ggaauaucac aagcaauuuc cuacaauuua ccaucucaga      420

aaacagcugg ccgacuccaa ggagaaagcu gaucuucggc ugaucuaucu cacccuggcc      480

cacaugauua aguaccgcgg acauuuccuu uacgaagagu cuuuugacau caaaaauaac      540

gauauucaga agaucuucaa ugaguuuauu agcaucuaug acaacacguu cgaaggcucc      600

agccugucug gccagaaugc ucaaaucgag gccauuuuca ccgacaaaau cuccaagagc      660

gcgaagagag aacgggugcu caaacuguuu cccgaugaga agucuacagg gcuguucucc      720

gaauuucuca aacugauugu cggaaaccag gccgacuuca agaaacacuu ugagcuugag      780

gaaaaggcuc cucugcaguu ccccaaggau accuacgacg aggaucucga aaaugugcug      840

ggccagaucg gggacgauuu ugccgaccuu uucguugcug ccaaaaagcu guaugaugcg      900

auucuccuga gcggaauccu gacagugacc gacccuucua cgaaagcccc gcucuccgcu      960

agcaugaucg agcgcuacga aaaccaccaa aaggaucugg ccgcucuuaa acaguuuauu     1020

aagacaaauc ugcccgagaa guaugucgag guguucuccg accagucuaa agacggcuac     1080

gccggguaua ucgauggaaa gaccacacag gaagccuuuu acaaauacau uaagaaccuc     1140

cugagcaaau ucgagggcac cgacuauuuu cuugauaaga ucgaaagaga ggacuuccug     1200

cggaagcaaa gaaccuucga uaauggcucc auuccucauc agauccaccu ccaggaaaug     1260

aacgcuaucc ugcgccggca aggggagcau uaccccuuuc ugaaagagaa caaggagaaa     1320

auugaaaaga uccucacauu ccgcauuccu uauuacguug gaccccuggc gagaggcaau     1380

cgggacuuug ccuggcuuac gagaaacagc gaucaggcua uccgcccgug gaauuucgag     1440

aaaauugugg acaaggccuc uuccgccgaa gauuuuauca acaaaaugac caauuaugac     1500

cuguaccucc cugaggaaaa gguccugccc aagcacagcc ugcuuuauga gacauucgcu     1560

guguacaacg aacucaccaa agugaaguuu auugccgagg ggcugcggga uuaccaguuc     1620

cuggacucug gacagaaaaa gcaaaucguc aaucagcucu uuaaagaaaa gagaaagguu     1680

accaaaaagg auaucauuca cuaucugcau acaguggacg gcuacgacgg gaucgagcuu     1740

aaaggaauug agaagcaguu caacgcgagc cuguccacgu aucacgaucu ccugaaaauc     1800

auuaaggaca agaaauuuau ggaugacucu aagaaugaag cuauccugga gaacaucgug     1860

cauacacuua ccauuuucga agaucgcgag augaucaaac agcggcucgc ccaauacgac     1920

agccuguuug augaaaaggu cauuaaagcc cugaccagac gccacuauac aggcuggggc     1980

aagcucuccg cuaagcugau caaugggauu ugcgacaaac agaccggaaa cacaauccuu     2040

gauuaccuga ucgacgaugg cgagauuaau cggaacuuca ugcagcucau caaugacgau     2100

gggcugagcu ucaagaaaau uauccagaag ucucaagugg uuggagagac ggacgacgug     2160

aaacaggucg ugagagaacu gccuggcucc cccgccauua agaaagggau ccuucagagc     2220

aucaagauug uugaugagcu cgugaagguc auggaccaug cucccgaauc uaucgugauu     2280

gagauggccc gggaaaacca gaccacagcg cgcggcaaaa agaacuccca acagagauau     2340

aaacggaucg aggauagccu gaagaaucug gccuccggac ucgacucuaa cauucugaaa     2400

gagaauccua ccgauaacau ccagcuuaag aaugacagac uguuucucua cuaccugcaa     2460

aacggcaagg auauguauac aggggaagcu cuggacauua aucagcuuag cuccuacgau     2520

aucgaccaca ucauuccgca ggccuucauc aaagaugacu cucucgacaa ccgcguccug     2580

accagcucca aggauaaucg gggaaaaagc gacaacgugc ccucucugga gguugugcag     2640

aagagaaagg cuuuuuggca acagcuccug gauuccaaac uuauuagcga acgcaaguuc     2700

aauaaccuga cgaaagccga gagaggcggg cucaaugagc gggacaaggu cggauuuauc     2760

aaacgccagc ugguggaaac cagacagauu acaaagcacg uugcgcaaau ccuggaugcc     2820

cgguauaaca ccgaggugaa ugaaaaagac aagaagaacc gcacagucaa aaucauuacc     2880

cuuaagucua accugguguc caauuucaga aaagaguuuc ggcucuacaa ggugcgcgaa     2940

aucaacgauu aucaucacgc ucaugacgcc uaccugaaug cuguugucgc caaagccauu     3000

cucaagaagu auccuaaacu ggagcccgag uucguguacg gcgaauacca gaaguaugau     3060

cuuaaaagau acaucagccg gucucgcaac ccuaaggagg uggaaaaagc uacggagaag     3120

uacuuuuucu auagcaaucu gcucaacuuu uucaaagaag agguccacua ugcggacggg     3180

acaauuguga agagagagaa uaucgaauac uccaaggaua ccggcgagau cgccuggaac     3240

acagaaaaag acuucgcuac cauuaagaaa auccugagcu auccccaggu uaauauugug     3300

aagaaaaccg aggaacagac agucggacaa aacggcgggc uguuugauaa uaacaucgug     3360

ucuaaggaga agguugugga cgccuccaaa cuuauuccga ucaagagcgg acuguccccu     3420

aaaaaguacg gcggguacgc ccggcccacg auugcuuauu cuguccucgu gaccuacaaa     3480

cagaauaaca agaucaagaa uacaaugguu ggaaucccug ugcugaccaa acucgaguuc     3540

gaaagagacc ccaaccugua ucuucgcaac cugggcauua auaacgucag ccacgugauc     3600

aagcucguga aauacacagu ccuggaguuu aagcauaaau augggaccua ccggagauuc     3660

auuguuucca aucaggaacu gaagcgcgcc aaccaaaucu uugugacgac aaagcagaug     3720

aaacuuauuu cucaguauac cacccuggau aagaaugagc acgacagccu ccugaaaaac     3780

aaucagcaac ucauccaguc caucuggaac caguuccugg aguuuaugcu ugcgacagau     3840

cuggugaaua agaaacagua cgacgaacug auucucacca acaaucaaac aaucgaggaa     3900

ugucggaaca uuaugaauag agucaucacg acccugucuu ucacaaacau uaaugcuggc     3960

cagaagaaaa ucaucuuuga ugacacccuu agccugccua uuaagcgcug gcaguucaca     4020

gauuccgaca agauggugaa aaagaucauu gagagcaccc ucauccauca gucuauuacg     4080

ggacuguacg aaacccggau cgaucucucc aaacugggcg aggacuga                  4128


<210>  4330
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4330
augaaggagu accacaucgg ccuggacauu gggaccagcu ccaucggaug ggccgugaca       60

gauucucagu ucaaacucau gagaauuaag ggcaaaacgg cuaucggggu ccggcuguuu      120

gaagagggaa agaccgccgc ggaacgcaga acauuccgga ccacacgcag acggcuuaaa      180

cgcagaaagu ggcggcugca uuaucucgac gagauuuuug cuccccaucu gcaagaaguu      240

gaugagaacu uccuucgcag acugaagcag agcaauaucc acccugagga cccggccaaa      300

aaccaggccu uuaucggcaa gcuccuguuc cccgaucugc ucaaaaagaa ugaacggggg      360

uacccuaccc ugauuaaaau gcgcgacgag cuucccgugg aacagagagc ucacuauccu      420

gugacaaaca ucuacaagcu gcgggaggcc augauuaaug aggaucgcca auuugaccuc      480

agagaagucu aucuggcugu gcaucacauc guuaaguacc ggggacacuu ccuuaacaau      540

gccuccgugg auaaauuuaa ggucggcaga auugacuucg auaaaucuuu uaacgugcug      600

aaugaggcgu augaagaacu ccagaacggc gaagggagcu ucaccaucga gcccuccaag      660

guugagaaaa uuggacagcu gcugcucgac acgaagaugc gcaaacugga ucggcagaag      720

gccguggcua aacuucugga agucaaggug gccgacaagg aggaaaccaa aagaaauaag      780

caaaucgcua cagccauguc uaaacucgug cugggcuaca aggcggacuu ugccaccguc      840

gcuaugguua acgggaauga guggaagauc gaucuuagcu ccgaaacaag cgaggacgag      900

auugaaaaau uccgcgagga acugucugau gcccagaacg acauccucac cgagauuacg      960

ucccuguuca gccagaucau gcugaaugaa auugugccua acggaauguc uaucuccgag     1020

agcaugaugg aucgguacug gacacaugag agacagcucg cugaagugaa ggaguaucug     1080

gccacccaac cggccucugc ucgcaaagaa uuugaccagg ucuacaacaa guauaucggc     1140

caggcgccca aagagaaggg guucgaucuu gagaagggac ugaaaaagau ucucuccaaa     1200

aaggaaaauu ggaaagagau cgacgaacug cuuaaggccg gcgauuuucu gccuaagcaa     1260

cggauuagcg cuaacggcgu gaucccccac cagaugcauc agcaggagcu cgacagaauu     1320

aucgaaaaac aagccaagua cuauccuugg cuggccacag agaaucccgc uaccggggag     1380

cgcgaucggc accaggccaa auacgaacug gaccagcucg uuuccuucag aaucccguau     1440

uacgugggac cucuggucac ccccgaggug cagaaggcga caucuggcgc uaaauuugcc     1500

ugggccaagc gcaaagaaga cggggagauu acgccuugga accuuuggga uaagaucgac     1560

cgggcugaaa gcgccgaggc uuucauuaag agaaugaccg uuaaagauac auaccugcuc     1620

aaugaggacg ugcugcccgc caacucccug cuuuaucaaa aguacaaugu ccucaacgaa     1680

cugaauaacg ugcgggucaa uggacgcaga cugagcgugg gcaucaaaca ggauauuuau     1740

accgagcucu uuaagaaaaa gaagacaguu aaagcgucug acguggccuc ccuggucaug     1800

gcuaagacca gaggggugaa caaacccagc guugaaggac uuucugaucc uaagaaauuc     1860

aauagcaacc uggccacgua ccucgaccug aaguccaucg ugggcgauaa ggucgacgau     1920

aauagauauc agauggaccu ggagaacauu aucgaauggc gcucuguguu ugaggauggc     1980

gagaucuucg cugacaaacu uacagaagug gaguggcuca ccgacgaaca gcggagcgcc     2040

cugguuaaga aaagauacaa ggggugggga cgccugucca aaaagcuccu gaccggcauu     2100

gucgaugaga acgggcaaag aaucauugac cuuaugugga auacagauca gaacuuuaug     2160

cagaucguga aucagccggu guucaaggag caaauugacc agcugaacca gaaagcgauc     2220

accaaugaug gaaugacacu ccgggaacgc gucgagagcg ugcuggacga ugccuauacg     2280

ucuccccaaa acaagaaagc uaucuggcag guugugagag ucguggaaga cauuguuaag     2340

gccgugggca augcuccuaa auccaucagc auugaguuug cccggaacga agggaauaag     2400

ggcgagauca cccgcucuag acggacacag cugcagaaac uuuucgagga ucaagcccac     2460

gaacucguca aggacaccuc ccugacagag gaacuggaga aggugcccga ucucagcgac     2520

cgcuacuacu ucuauuuuac ccagggaggc aaagauaugu acacggggga cccuauuaac     2580

uucgacgaaa ucuccaccaa guaugauauc gaccauauuc ugccccaguc uuuuguuaaa     2640

gaugacagcc uugauaauag agugcugaca ucccggaagg agaacaauaa aaagucugac     2700

caggucccgg cuaagcucua cgcggccaaa augaagccuu auuggaacca acugcugaaa     2760

cagggacuua ucacccagcg caaguucgag aaucucacaa uggaugugga ccagaccauu     2820

aaauacagaa gccugggcuu ugugaagcgg caacuggucg aaacgagaca gguuaucaag     2880

cucacagcua acauucuggg guccauguau caggaggccg gaaccgauau caucgaaaca     2940

cgcgccggcc uuaccaaaca acugcgggag gaguucgacc uccccaaggu gagagaggug     3000

aacgauuacc accaugcugu cgacgccuac cugaccacau uugcggggca guaucugaau     3060

cgccgguacc cuaaacuucg gagcuucuuu guguauggcg aguacaugaa guucaaacac     3120

ggaucugacc ugaagcuccg caacuuuaau uucuuucaug aacugaugga gggcgauaag     3180

ucccagggga aaguugugga ccagcaaacg ggagaacuca uuaccacacg ggaugagguc     3240

gcugacuauu ucgauugggu gcaggaccug aaacuuaugc ugguuaccaa ggagcucaag     3300

agaagcaacg gcaaacuguu cgaugccaca auccagaaug ccaacuacac caagcugaau     3360

gaacgcaaga aaguguuugu cccccuuaag aaagagcugg acaagacgcu cuaugggucu     3420

uacuccggca uucagagcuc uuacaugaca aucgugaaaa aguucgauaa aaagggaagc     3480

accuaugcug uucaacggau uccugugaug uacuccaagc ugcucgaacu ggagaaagaa     3540

cagagcaaac uuaacagacu gguccagucu aucgugaccu ccgaccccca ggccccgaau     3600

aacuuugagg ugcucaugcc uugcguccug agcgggcaac ugguuauuga uaauggccgg     3660

aaguucacac uuaccuccua ucgcuaccag uguucuacag cgcagcuguu ucuccccaag     3720

aaagaccuga aguauaucca gaacaauuuc aaggagcuca aaaagacgcc cgauaaacaa     3780

gacaagaucu uugaaaccau uuacgacgau auccugaaac agguggacaa guauuucagc     3840

cuuuacgaua uuaacaaguu uagauccaaa cugcacgacg gaaaggauaa auuccuggcu     3900

cucgagaauu uuggcgaaaa ggagucuaaa gacaagguga ucacaaagau ucugcggggg     3960

cuucacgcca acgccgaacg caccagccug aaagagaucg gacucuccac accucugggc     4020

caguuccaau cuggggaggg aggcaucacc cucagcgaua acacgaagau uaucuaccag     4080

uccccgaccg ggcuguucga aagacauguc uaucuuaaag accuguga                  4128


<210>  4331
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4331
augagaaaga accaggagcc cuacaauauc ggccuggaca uugggaccag cuccaucgga       60

uggucuauua ugaacgauaa uuucgaccuc augcggguga aaggcaagaa agggaucgga      120

guccgccugu auaacgaagg ccaaagcgcc gcugagagac ggaugcaccg cacagccaga      180

cggcgcuacg ggagacggaa guggcgccuu agacugcucg aagauuucuu cgacgagcau      240

auggcggaag uugaugacac guuuuucgcu cggcugaaag auuccaauau uucuccuaag      300

gacgauaaga aauaucgcaa gagccuucug uuuccgaaau ccaagggagu gaccuaccag      360

gacgauggcg aguucuauaa aaaguacccc acaauguauc accucagaua cgcccugaug      420

accgagcauc ggaaguuuga ccugcgcgaa aucuaccucg ccuuccacca uauggugaaa      480

uauagaggca acuuucugua cgauacaucu gucgacagcu ucgaggcuaa gaaucuugac      540

aucaaaggga aguuugauga aauuaacgac cugcucucca gcuauaccga uuucuacgug      600

gacaauucua acgccgcucu gguugagucc auccuucugg agaaaaauac aacccggaag      660

gauaaaagca agaagauugc caaacuccug cacguggaag acaaggagaa aggaaagaac      720

aaaaaggcga aggaucuggc cacgcagauc ucuaaugcug uccucggccu gaaaugcaac      780

uuucaccuua uuuucgaacu gcagcaaaag uauuccuucg accucuguag cgagaaaacc      840

gaagagaaua ucgccaagcu gucugaggug cuugaugaaa accagaaaau gcugcucaug      900

auucugaagg agguucagga ccaggugaug cugaacgcuu uugucccuuc cgggaugagc      960

cucuccgaag ccaugaugca aaaguacgau gacuauggag agcagcugaa aaucuaccau     1020

gaacuugagc acucugugag cgacgauucc gcggccaagc ugagaggcgc uuaccgcgac     1080

uauaaugaua acaucauuaa acggaucgac ggggauaaua agaaagacac auucuacaag     1140

agagugaaga acauucucac caaaaucagc aaggaguaug ucgaucagga cguucuggaa     1200

ucuugcggaa aacuuaagaa acugauugau gagaauaagc ucuuuauccg ccagcggaca     1260

gccgcuaacg gcgugcugcc ccaucaacug caccagaucg aaaugagaaa aauuaucgac     1320

aaucagaagg aguacuaucc uuggcucgcc gaacccaacc cuaaugagaa gcgccgggug     1380

uacuccaaau auaaggucga ggaacugauu gccuucagaa ucccguacua cgugggcccc     1440

cuuguugauc cuaacaaugc ugacaaaaac aaggaggcgc gcuuuagcug gauggugcgg     1500

aaaaaggaug gggaaauuac ccccuggaau uucuaugaca aggucgacug ggccgagucu     1560

gcuaacaauu uuaucgagag aaugaaaagc aaggauacgu accugcucgg agaagacgug     1620

guuccuaaag aguccaugcu guaucaaaag uacgaagugc ugaacgagcu uaacaaucuc     1680

cgcaucaacg auucuggccu gagcgacucc uucgaagaug ucaaacugaa gcaggccauu     1740

uauaaugacc ucuuuaagaa acagaagauc gugaaaauua cagaucugca gaacuaccuu     1800

guccaaaauc acaaguaucu ggugaaaccc aagaucagcg ggcucgccga cgagaaccgg     1860

uuccugucuu cccugagcac cuacucugau cuuaagacaa uuuuuggaga caaaguugau     1920

gacagaaccu acuucaauga uuuugagaag augguggaau auuccaccgu cuucgaggac     1980

ggccaugacu acaaccagaa acucgaugaa uauacauggc ugacgaagga ggaaaaauac     2040

aagaucggga aaaagcggua ucgcggaugg ggcaagcuga gcaaaaagcu ccugaccggc     2100

cuuagagaca aaaauaacca cacaauuaug gauaaucugu gggagaccaa ccggaauuuc     2160

augcagaucc agacagcuga cgaguuuucc aagcaaaucg ccgaagagaa cgaaagacau     2220

cucaaagggu cugugagcga ugcgauuaau gacauguaca ccuccccggc uaacaagaag     2280

gccauccgcc agguucugcg gguggucgau gacauucaga aagccauggg auaugcuccu     2340

ucuagcaucu cccuggaguu cgccagagaa gauggcccca gcgugcgcac ggugucuaga     2400

gcuaaccgga ugaaguccau uuacgagaaa uacgccagcg agguuucuga agaggucaug     2460

aaggaccuug auggggugau caaagacaag aagggacuca augaccgccu guaucuguac     2520

uuugaacagc aaggcaaaga uauguauucc gggcacccuc ucgacuucga uaaggugauc     2580

agcggccagg aguacgacau ugaucauauc cugccccagg cggucauuaa agacgauucu     2640

cuugacaaca gagugcugac aaccaaggcc cucaauaacg auguuaaaag caagggagug     2700

cccugucgga uguuuaaugg caugcacucc uucuggaaga accuguauga caaaggguuu     2760

aucagccgca gaaaguucaa uaaccugacc acaaauccug agaacauuga uaaauacaag     2820

augaaaggau uugucaaucg gcagcuugug gaaacccgcc aaaucaucaa gcucguugcu     2880

aacgugcuga augacaagua ucagaacgau gacgucgaca uuaucgaggu gagagccgaa     2940

cugacacacg auguucggaa acauuucaag uuuuacaaaa aucgcaacgu gaacgacuac     3000

caccaugcuu ucgaugccua ucucacgucu uuuauuggcc acuaccuguu caagaaauau     3060

ccgaaucuua gaccccuguu cgacuacaac gauuuuauga aggucuccga caauguguuc     3120

aaacagcucc gggggaacaa uuuucuggga gaguuccuga acaagaccgg cgauaucauu     3180

agcacagacg ggaauuuugu gcuuaacaag gaagagauga ucaauaaacu gaacaaggcg     3240

uaugccuuca aaaagauccu cgucaccaaa gagguuggcc aaagaacagg agcuauguuu     3300

aaugaaaccc gcuacccugc ccccaacucc cggaaggcug cccugaagua uagacgcaug     3360

ccuaaacccg auucucucau uagcgugaag gacuacaaaa auacggauau cuacggcggg     3420

uauuccggaa agaacgacgc cuacauggug auugucgaua ugggcaaaca guauauggug     3480

guuggggugc cgguccggua caccgagaag cuggacaaac uuagaaucaa acacucugaa     3540

cuguaucgcg acgagcuccg gaaggugcug agcagagaua aaacacugcu ugacuccaag     3600

ggaaauguua aacgcuucga uauugugcug gaccgggucc ucuacggcca gcugaucgaa     3660

gaugggaacg agcucuuuac ccugggcucu agcaaguaua agagaaauuu ccggcagcuu     3720

uuucuggaca aacaaugcau ugagauccuc gauuccagcg cuacaccuca gcccaccgac     3780

gaagaacuga ucugggugua cgaucagauu cuggacaagg uugauaaaua cuucgaacuu     3840

uaugacauca accgccagag agagacgcug cggaagggac gcgcggccuu cuguucucuc     3900

ccuaacauuu ccaugaaaaa ggacgaugug acaaagaaga agauccugaa ugaaauucuc     3960

gucgcucugc augccaacga gagcgagucu aauugcgaga aaaucggcaa gaaaguguuu     4020

gggagacuuc aagugcccgg aggcaucaag cuguccaaag aggccaagcu gauuuaccag     4080

agcccuaccg ggcucuuuga acgggucguu uaucugaagg accuuuga                  4128


<210>  4332
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4332
auggagaaca agaaauacag caucggccug gacauuggga ccuccucuau cggauucgcc       60

guggucgaug aaaauaacag aguuaugcgg gugaagggca aaaaugcuau uggggugcgc      120

cucuuuagcg agggacagcc cgccgcggac agacgguccu uccgcacaac gagacggcgc      180

cugucuagac ggcgcuggag acuucggcug cuccgcgaaa ucuuugagcc uuauauuaac      240

ccggucgaug aggcuuucuu uaugagacug aaggagagca aucuuucccc caaagacgcc      300

aacaagcacu acucuggcga uauccuguuc aauaaccaaa ccgaccagga uuuuuaugag      360

aaguacccua caaucuauca ucuccggcac gcccugauga ccgaacaucg caaauucgac      420

gugagagaga uuuaccuggc uauccaccau auuguuaagu uucgggggca cuuccucaau      480

gccgcuagcg ccuccaacuu uacaguggga aaacugaauc uugaaggcaa guucgaugcg      540

cugaacgaga ucuaucagag aguccucacc gacgaucccu uugcccugcg cacagacaau      600

cuugaucaca uuaaagagau ccugauugac aacaagcgga gccaggcuga cagacaacgc      660

acccucacgg gcgaugugua ccagucuucc gaagagaagg aaaucgagaa acggaauaag      720

gccaucgcua ccgaaauucu gaaagccgcg guugggaaca aggccaaacu gaacgugauc      780

acaaaugucg agguggacaa ggaggcugcc aaagauugga gccucaccuu cgacucugaa      840

acaauugaug acgagcuggc uaagguggau ggacagauga ccgacgaugg cagagaaauc      900

auugagaucc uucgcucccu guacagcggg aucacgcucg ccgccauugu cccugaaaac      960

cauacacugu cucaguccau gguugagaag uauaauagcc acaaagcuca ucuuaagcug     1020

cucaaagacc ugaucaacaa uaugaccgau acaaagaaag cgaagaaccu gcgggccgcu     1080

uacgacggau auauugacgg cgugaagggg aaagugcuca ccagagauga cuucuacaag     1140

caaauccagg gaaaucugga ugacuccgcc gaggcccaga aaauucagga ugucauggac     1200

caagaugaau uuaugcccaa gcagcgcacc aaagcuaacg gcucuauccc ucaccagcuu     1260

caacagcagg agcuggacca gaucguggaa aaucaaaagg aguauuaccc guggcucgcc     1320

gaagagaacc ccaaugagac acggagaggc guugcgaagu auaaacugga ugaacuugug     1380

acguuccgcg ucccuuacua cguggggccc augauuaccg cugaggacca ggaaaagcag     1440

agcggagccu ccuuugccug gaugaaacgg aaggagccug gcaacaucac acccuggaau     1500

uucgaucaga aaguugacag aauggcuagc gccaaccaau uuauuaagcg gaugaccaca     1560

accgauacgu aucugcucgg ggaggacgug cugccggcuc agucucugcu cuaccagcgc     1620

uucgaagucc ugaaugagcu uaacaagauc agaauugacc ggaaaccuau cuccauugaa     1680

cugaagcagc aaaucuuuaa cgaucucuuc aaaaaguuua aaaaugugac aaucaagcac     1740

cuccaggagu aucuggucgc caccggaggc uacgacaaaa gaccccgcau ugaugggcuu     1800

gcggacgaaa agcgguucaa cagcggacuc ucugccuauc augaucugca cggcauccug     1860

ggcgcugagg ugguugacgc caaugauaag caggacgauc ucgagaaaau uaucgaaugg     1920

agcaccauuu uugaggacaa gaaaaucuac agagcuaagc uggaugaacu uacauggcug     1980

accgacuccc agaaagagaa gcucgccaca aagcgcuauc aagggugggg aagacugucu     2040

aaaaagcugc uuagcggccu ccggaacucc gaucaucgca gcauccugga cauucugugg     2100

aucacgaaug aaaacuucau gcagauucag gcggacccug auuuugccga gcucgugaaa     2160

aaugcuaacc aagggaugcu gggaaagaau aacucucagg acgucaucaa ugaucuuuac     2220

accagccccc agaacaaaaa ggccauuaga cagauccugc ucgugguuca cgacauccaa     2280

aaugcuaugc auggccaggc ccccgccaag auucacgugg aguucgcucg cggggaagaa     2340

cggaacccua gacgguccau ccagcgccag caccaaauug aagcggccua cgauaaaguc     2400

acaucugacc ugcuggugga ugcuggcgug agacaggagc uuaaggacgc caucaaugau     2460

aaacgggacu ucaaggaucg ccucuuucug uauuucaugc agggaggcag agacauuuac     2520

aacggggaac cgaucaacau cgaccggcug uccagcuaug auauugacca uauccucccc     2580

caggccuuug uuaaagauga cucccugaau aacagagucc uuaccgaugc ugagggaaau     2640

cgccggaagu cugacgccgu gccuagcgag cuguucggcg cgaagaugag auccacaugg     2700

gaacucaugc gcgagcaagg gcugauuucu aaagcuaagu accggaaccu gaccaugaau     2760

cccgaaaaca ucaaugccca cacggagaac ggauuuauua auagacagcu uguggagacc     2820

cgccagguca ucaaacucgc cgugaacauu cuggcugaug aauauacaga ggacacccaa     2880

aucguuagcg ugaaggccga ucugucccau cagaugcggg aagacuucga gcuccugaaa     2940

aauagagaug ucaacgacua ccaccacgcg uuugaugcuu aucuugccgc cuucaucggc     3000

aauuaccugc ucgaacgcua cccuaagcug cagagcuauu uuguguacgg ggacuucaaa     3060

aaguuuucuc agaaggacac aaaaaugcgg agauucaacu uuauuuauga ucugaagcau     3120

uccgaccaaa ucgcugauaa aaauggcaag aaaauuuggc acaagaccaa cgaccuugau     3180

uacauccggc aucuguucgc cuauaagaaa auucucauca gccacgaggu uucugagcgc     3240

agaggagcuc uguacaacca gacgguguau aaggccuccg acgauaaagc gagcggccag     3300

gggucuaaga aacucauccc cauuaaggcc aauaagccga cagaaaucua cggaggcuac     3360

accgggaaaa cacuggcuua uauggccauu gucaagguga ucaaaggaaa gaaaauugcu     3420

uacaagguug ugggcguccc uaccagcgcc cuugugcagc uggccgggau cgagcauggc     3480

ucccaagagg aacucaaccg gcuguaugac gugcugaagc cccaguucac ccagcuuaaa     3540

aagaauaaca aaacaggaga aaucagcgag acggucucua aguuugagau uguuaucccu     3600

cgcgugaaau accagcaacu gguggaagau gacgauaagc uguucaugau uggcuccgac     3660

accuaucccu acaaugcuca gcagcugguc cucagcgagg gggcgcugaa ggugaucaac     3720

aauguuuaua gaaaaggaga aaacuccgau aagcaggagu cuuacaaacu ugacgacgug     3780

uacgaugaaa uuaucauugc caugaagaaa uauuuuccuc uguacgacaa ucggggcuuc     3840

agagagaagg ucgcugaggg gaaaagcaca uuugaaaacc uccccaucuc caaugaguuc     3900

aagaacggaa agcugaccaa aaucggcaag aaagaaguga uuaaucgcau gcugcaaggg     3960

cuucacgcca acgccacaau gaccgaucug aagauccucg gcauuaaauc uccguuugga     4020

uucaugcagc agccuacggg caucgcucug agcgagaaug cccaacucau uuaucagucc     4080

cccacagacc uguuugagcg gagaguucag cuuaacaaga ucaaguga                  4128


<210>  4333
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4333
augaagaaaa uccugggccu cgaccugggg accacaagca uuggaugggc ccuuguguac       60

gaguccgaaa acacggauga gaagucuagc aucgucagac ugggcguucg ggugaauccc      120

cucuccgugg acgaaaaagg gaacuucgag aagggaaaag cuauuaccac aaaugccgau      180

cgccagcaaa cccacggcgc gagaaucaac cugcagcggu auaagcugcg cagagaaaau      240

cugaaggacu gccuccagaa acaggggugg auuggaacag aggcucugug ugaggaaggc      300

aagaccucua cauuugagac cuacaugcug cgggccaaag ccgcugaaga ggagaucagc      360

cuccaugaac ugugccgcgu ccuuuucaug augaacaaga aaagaggcua uaaguccaau      420

cggaaggcca acaauaaaga ggaugggaag cuguuugacg gaaugacggu ggcuaaaaag      480

cucuacgagg aacaccugac cccugccgaa uauucuuucc aacuucugag cuccggcaaa      540

aaguuuaaac aggaguacua ucgcagcgau cucaaggagg aauuugacag aaucuggaac      600

gagcagcaga aguacuaccc ggaaauucug acagaugacu uuaaacaaca gcugcagggg      660

aagucuaaag gaaauaccuc caagaucuuc cucggcaaau augggauuua cagcgcggau      720

cugaagggac uugaccggaa gcugcagccc cucaaauggc gcguugaggc ccugaagcaa      780

cagguggaua aagaaguccu ugcuuuugug aucucugacc ugaacggcca gauugccucc      840

acaagcggcc uccugggggc uaucucugac agauccaagg agcuguauuu caauaaacaa      900

accguuggac aguaccucug gacgagccug aaggagacac cucauguguc cauuaagaac      960

aaacccuuuu aucggcagga uuaccuugac gaauuugaga agaucuggga aacccaggcc     1020

acauaucaca aagagcugac cccugaacuc aagcaagaga uccgcgauau uaucauuuuc     1080

uaccagagac cccugaaauc uaagaagggc cuuaucagcg ucugugagcu ggaacagcgg     1140

aaagugaagg ugaccauuga cgggaaagag aaggaaaucu ccaucggacc uaaagucgcg     1200

cccaagagcu cuccgauguu ccaggaguuc agaauuuggc aaaaucucaa caacauccug     1260

cugauugaua aaggcaauaa cuccauccau ccucucuacg aggaagagaa gaaucugcuu     1320

uaugaggaac ugagcguuaa ggaaaaacuc ucuaaggccg aggcucugaa agugcugaac     1380

aagaauagca aacaguggga ccuuaacuac aaggagauug aagggaaucg cacacaggcc     1440

gugcucuaug auugcuacaa ccggaucauc gucaugacgg gacacgagga auauaauuuu     1500

aagaaacuga aggcuuccga gauuagaucu uacgugaaaa ccacauucac cggccugggc     1560

uuuaacacag acauccucga guucgauagc ucccugaccg ggcacgaacu ugagaagcag     1620

cccauguauc aacuguggca ucuccuguac agcuacgaau cugacaauuc ccgcacggga     1680

aacgaugccc ugcuucggaa acucgagaca gccuuuggcu ucccuaagga auaugcugcg     1740

guucugagcg acguggucuu ugaggacucu uacgggaauc uguccgugaa ggccaugaga     1800

aaaauucucc cccaucugca ggcuggacuu aacuauagcc aggccuguga agaggccggc     1860

uacaagcacu cccgccgguc ucugaccaaa aaugagcucg aucagaagga auauaaagag     1920

aagcuggaac ugcuuccgaa gaacagccuc cacaacccug uuguggagaa aauccugaau     1980

caaaugauua acgucaucaa ugcugugauu gacgaguacg ggaagcccga ugaaaucaga     2040

aucgagcugg cccgcgaacu caaaauuggc gcggcugagc gggaaaacau gaccagaucc     2100

aucaaucagg gaaacgccga gaauaagcgc auucgggaga uccuggaaaa agaguucgcc     2160

cuuucuuaua uuagcagaaa cgacaucauc aaguacaaac uguacgagga acucaagucc     2220

aauggcuuua agacacugua uagcgauacc uacauuucua aagacaagcu guucuccaaa     2280

gauuuugaca ucgaacacau uaucccuaag gcucggcuuu ucgaugacag ccucucuaac     2340

aaaacaauug aggcccgcga ugucaauauc gcuaagucca acaagacggc cuuugacuuc     2400

aucagcgaga aauaugauca gcaagggauu gaagcguaca agaaaaagcu ggacauccug     2460

cuccaggacg gaucuauuag caaagccaag uauaauagac ugcuuaugaa ggaggcugau     2520

auccccuccg gcuucauuga ccgggaucug agagacaccc aguacaucgc caaaaaggcu     2580

uaugaaaucc ucagcgaucu ggugcgcaac guuacaccca ccacaggggc cgugaccucu     2640

cggcugagag aggacuggca gcuugucgau gugaugaaag aacugaauuu ugagaaguac     2700

caaaaacucg gacugguuga ggugauugaa gaccgcgaug gcagaaagau caagcggauu     2760

aaagacugga cgaagcgcaa cgaucauaga caccaugcga uggacgcccu caccaucgcu     2820

uucacaaaac cuuccuacau ucaguaucug aacaaucuua acgcccggag cgauaaaaau     2880

uccucuaucu acgggauuga gaacaaggaa cugcaccgcg aggacaauaa gcucagauuu     2940

aacgcuccgc ugcccaucaa ugaguuccgg gccgaagccc gcagacaucu uaagagcauc     3000

cuugucuccg ugaaagcuaa gaacaaagug augacccaga auguuaacgu cauuaagaca     3060

aaacacggcg ugaucaagaa agugcagcug accccucggg gagagcucca caaugcgacg     3120

uuuuauggca caaagaagcg ccccauuauc aaaaugguca aggugggggc cgcucuggac     3180

gaaguuacca uuaaaaaggu gucuagcucc uucaucagag aggcccuccu ggaucggcuu     3240

caugaauaca acggagacgc caaaaaggcu uuuacaggca gaaauucuau cgauaagaac     3300

ccuauuuaua ucgacgagga gcacaaaaag accguccccg cccuggugaa aaccauugaa     3360

ugggagaugu accauccgac acgcaagcuc aucagcccug aucugaaaau ugacaagguu     3420

aucgauaagg ggauccggga aauucugaaa acgagacuug cgaaguucaa uggagacgcu     3480

caaaaagccu uuuccaaccu ggaugagaac cccaucuaua aggacgaagc caaaaagauu     3540

acccucaagc gcgugagccu ggagggcguc ucuuccguga ucccucucca cacacugaaa     3600

gaucgggagg ggaaacuugu ugugggcaag gacggaaagc ccguccuggg cgacuacgug     3660

cagaccagca auaaccacca uauugcuuuc uacauggaug aaaaugggaa ccugcaggac     3720

aauguggucu cuuucuucga ggccgcggaa agaaaauccc agggaguucc ugugaucgau     3780

aagaacuaua aucacgacaa aggcuggaac uuucucuuca caaugaagca aaaugaguac     3840

uucguguuuc ccaacgaagu caccggguuc aauccgagcg agguggaucu gauggacgag     3900

gcuaacuauu cuauuaucuc cccuaaucuu uaccgcguuc agaagaugag caaagccucc     3960

ggacgggauu auugguuuag acaucaccug gaagccaugc ucaacgacga ucccaagcug     4020

cgcaauagca uguucaaacg gaucacgucu cucaaggcuc uggagggcau ugugaaaguc     4080

agaaucaacu gcauugggaa gaucguggcc guuggcgaau acgacuga                  4128


<210>  4334
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4334
augagcaaca aguccuacuc uaucggccug gacauuggga ccaauagcgu gggaugggcc       60

gucaucacag augacuauaa aguucccucc aagaaaauga aggugcucgg caacacggau      120

aaacacuuca uuaagaagaa ucugaucggg gcccuucugu uugacgaggg aaccacagcu      180

gaagauagac ggcucaaacg caccgcgaga cggcgcuaca caagacggaa gaaccgccug      240

agauaucuuc aggagauuuu cucucaagaa aucagcaaag uggacuccag cuuuuuccau      300

cggcuggaug acuuuuuccu cgucccugag gauaagcgcg gcagcaaaua cccgaucuuu      360

gcuacccugg uggaagagaa ggaguaucac aagaaauucc ccacaauuua ccaucugaga      420

aagcaccucg ccgacuccaa agaaaagacc gaucugcggc uuaucuaucu ggcccucgcu      480

cauaugauua aauaccgcgg gcacuuucug uacgaggaau cuuucgacau caagaauaac      540

gauauucaga aaaucuuuuc ugaguucauu uccaucuaug acaauacguu ugagggaaag      600

agccuuucug gccagaacgc ccagguugaa gcuaucuuca ccgacaagau uuccaaaagc      660

acaaagagag agcgggugcu gaaacucuuc ccugaugaaa agucuaccgg ccuguuuucc      720

gaguuccuga aacucaucgu cgggaaucaa gccgacuuua agaagcacuu cgaucuggaa      780

gagaaagcgc cccuucaguu uagcaaggac acauacgaug aggaccugga aaaccuccug      840

ggacagauug gcgaugacuu cgccgaucuu uuucuggugg cuaaaaagcu cuaugacgcc      900

auccugcugu ccgggauucu caccguuaaa gaucugucua cgaaggcucc ucuuagcgcc      960

uccaugaucg agcgcuacga aaaucaucag aaggaccugg cggcccucaa acaauucauc     1020

cagaacaauc ugcaggagaa guaugacgaa guguuuagcg aucagucuaa agacggauac     1080

gcuggcuaua uugaugggaa gacaacccaa gaggccuucu acaaauacau caagaaccuu     1140

cuguccaagu uugagggagc ugacuauuuc cucgauaaaa uugaaagaga ggacuuucug     1200

aagaaacagc ggacauucga uaauggcagc aucccccacc agauucaucu gcaagaaaug     1260

aacgccauca ucagacgcca gggcgagcac uacccuuucc uccaggaaaa caaggagaaa     1320

auugagaaga uccugaccuu ucggauuccg uauuacgucg ggccccuugc cagaggaaau     1380

ggcgacuucg cuuggcugac ccgcaacucu gaucaggcga uccggccuug gaauuuugaa     1440

gagauugugg accaagccag cuccgcugaa gauuucauca acaaaaugac aaauuaugac     1500

cucuaccugc ccgaggagaa ggugcugccu aagcacucuc uucucuauga aacguuugcc     1560

gucuacaacg agcugaccaa aguuaaguuc auugccgaag ggcugacaag auaccaguuu     1620

cucgauaaaa agcagaaaaa ggacaucuuc gacaccuuuu ucaaggcuga gaauaaacgc     1680

aaggugacag aaaaagauau cauucauuau cugcacaacg uggacggaua cgauggcauc     1740

gagcuuaagg ggauugagaa acaguuuaau gccagccugu ccaccuauca ugaccuccug     1800

aagaucauua aggauaaagc guucauggac gauagcaaga acgaagagau ccuggaaaau     1860

aucauucaca cgcuuacaau cuuugaggac cgggaaauga uuaaacaaag acucgcucag     1920

uacgauucuc uguucgacga gaaggucauc aaagcccuga cccgccggca uuauaccgga     1980

uggggcaagc ucuccgccaa gcugauuaac ggcaucagag auaaaaagag cgggaaaaca     2040

auccuugacu accugauuga ugacggagag aucaaucgca acuucaugca gcucauucac     2100

gacgauggcc ugucuuuuaa ggaaaucauu cagaaagcuc aaguguucgg gaagaccaau     2160

gacguuaaac agguggucca ggagcugccc ggauccccgg ccaucaagaa gggcauccuu     2220

cagagcauua aaaucgugga ugaacucguu aaggugaugg ggcacgcucc ugaguccauu     2280

gucaucgaaa uggcccggga gaaccaaaca acggcgagag gcaaaaagaa cucucagcag     2340

cgguauaaac gcauugagga cagccugaag aaucuggccu ccggacucga uucuaacauc     2400

cugaaggaaa aucccaccga caacauucaa cuucagaaug auagacuguu ucucuacuac     2460

cugcagaacg gccgggacau guauacaggg aaaccucugg agaucaauca gcuuagcaac     2520

uacgauaucg accauauuau cccccaggcu uucauuaagg augacucccu cgacaauaga     2580

gugcugacca gcucuaaaga uaaccgcgga aaguccgaca auguccccag ccuggaagug     2640

guugagaaaa ugaaggccuu uuggcaacag cuccuggauu cuaagcugau cuccgagcgg     2700

aaauucaaca aucugacaaa ggcugaaaga gagcgcggcg ggcucaacga acuggacaaa     2760

gugggauuua uuaagagaca gcuggucgag acccggcaaa ucacgaaaca cguggcccag     2820

uuccuugaug cgcgcuuuaa uaaggaaguu accgagaagg acaaaaagaa cagaaacgug     2880

aaaaucauua cacuuaagag caaucucguc ucuaacuucc ggaaagaguu uggccuguau     2940

aaggugcgcg aaaucaauga uuaccaucac gcccacgacg cuuaucucaa cgccgugcug     3000

gcuaaggcca uucuuaaaaa guacccuaaa cuggagccgg aguucguuua uggggauuac     3060

cagaaguacg accuccagag auauaucagc aaaucccggg aacccaagga agucgagaaa     3120

gccacccaaa aguacuucuu uuauagcaau cugcugaacu ucuuuaagga ggaagugcau     3180

uacgcugaug gcacaauugu gaaacgcgag aauaucgaau auucuaagga caccggagag     3240

aucgcgugga acaaagaaaa ggauuucgcc acggucaaaa aggugcuuuc ccugccucag     3300

guuaauauug ugaagaaaac agagguccag accggcgggu uuagcaagga guccauccuc     3360

cccaaaggaa acucugacaa gcugauuccu agaaaaacaa aggaaauccu cugggacacc     3420

accaaguacg gcggguucga uagccccgug auugcuuauu ccauccugcu uauugccgac     3480

aucgagaaag gaaaggccaa aaagcugaaa acaguuaaga cgcucguggg caucaccauu     3540

auggaaaagg cuacauuuga gaaaaauccg aucaccuucc uggagaacaa gggguaccac     3600

aauguccgga aagaaaacau ucugugccuu ccuaaguacu cucuguuuga gcucgaaagc     3660

ggccgcagac ggaugcuggc cuccgcgaaa gagcuccaaa agggaaauga aaucgugcug     3720

cccguuuauc uuacaacccu gcuguaccau ucuaaaaacg ugcacaagcu cgaugagccu     3780

gagcaucugg aauauauuca gaagcacaga uacgaguuca aagaccuucu gaaccucguc     3840

agcgaauuuu cccagaagua ugugcuggcu gaugccaauc ucgagaaaau caagaaccug     3900

uacgccgaca augaacaggc ugauaucgag auucuugcca acagcuucau caaucugcug     3960

acguuuacag cgcucggcgc ucccgccgcc uucaaauucc uggggaagga cguggaucgc     4020

aagcgguaua ccaccgucuc ugagauucuu aacgcuacac ugauccacca auccauuacc     4080

ggacucuacg aaacaagaau cgaccugagc aaacucggcg aggauuga                  4128


<210>  4335
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4335
auggagaaga aauacagcau cggccuggac auugggacca acuccguggg augggccguc       60

aucacagaug acuauaaggu ucccucuaaa aaguucaaag ugcucggcaa uacgaacaga      120

aagagcauua agaaaaaucu gaugggggcc cuucuguuug auuccggaga aaccgcugag      180

gcgacacggc ucaagcgcac cgcuagacgg cgcuacacaa gacggaaaaa ccgcaucaga      240

uaucugcagg aaauuuucgc caaugagaug gccaagcugg acgauagcuu uuuccaacgg      300

cuggaagagu cuuuucucgu ggaggaagac aaaaagaacg agcgccaccc uaucuucggc      360

aaucuggcug augaagucgc cuaccauaga aacuauccga ccaucuacca ucugcggaag      420

aaacucgcug acucccccga gaaggccgau cugcgccuua uuuaucuggc gcucgcccac      480

aucauuaaau uuagagggca cuuccugauc gagggaaaac uuaaugcuga aaacucugac      540

guggccaaac uguuuuacca gcucauucag acauacaauc agcuguucga ggaaagcccu      600

cuggaugaga ucgaaguuga cgcuaagggc auucucuccg cccggcugag caaaucuaag      660

cgccuugaga agcugaucgc gguguuuccc aacgagaaaa agaauggccu cuucgggaac      720

aucauugccc uggcucuugg acugaccccu aauuuuaaau ccaacuucga ucucacggaa      780

gacgccaagc ugcaacugag caaagacacc uaugaugacg aucucgacga gcugcuuggc      840

cagaucgggg aucaguacgc ugaccuguuc ucugccgcca agaaccucuc cgaugcuauu      900

cugcuuagcg acauccugag aucuaauucc gaagucacaa aggcgccccu cagcgccucc      960

auggugaaac gguaugauga gcaucaccag gaccuggcuc ugcucaagac ccugguuaga     1020

caacaguuuc cugaaaaaua cgccgagauu uucaaggaug acacaaagaa cggauaugcc     1080

ggcuacgugg ggaucggaau caagcaccgc aagcggacca cgaaacuugc uacacaggag     1140

gaauuuuaua aguucauuaa accgauccug gagaagaugg acggcgccga ggaacuccug     1200

gcgaaacuga auagagauga ccuucuccgc aagcagcgga ccuuugauaa cggcucuauu     1260

ccccaucaaa uccaccugaa ggaacugcau gcuauucuca gacgccagga ggaguucuac     1320

ccuuuucuga aagaaaaucg ggagaagauc gaaaagaucc uuacauuccg cauucccuac     1380

uaugucgggc cucuggccag aggaaacagc cgguuugccu ggcucaccag aaaguccgag     1440

gaggcuauca cccccuggaa uuucgaagag gugguggaca aaggcgccag cgcucagucu     1500

uuuauugaac gcaugacaaa cuucgaugag caacugccga auaagaaagu ccugccuaag     1560

cacucccuuc ucuacgaaua uuuuacgguu uacaacgagc ugaccaaggu gaaauaugug     1620

acagagcgga ugagaaagcc cgaguuccug agcggggaac agaaaaaggc caucgucgac     1680

cuccuguuca aaaccaaucg gaaggugaca guuaagcagc uuaaagaaga uuacuuuaag     1740

aaaauugagu gcuucgacuc uguggaaauc auuggagucg aggaucgcuu uaacgcgagc     1800

cugggcaccu aucacgaccu ccugaagauc aucaaagaua aggacuuccu ggauaaugag     1860

gaaaacgagg acauucuuga agacaucgug cucacgcuga cacuguuuga ggauagagaa     1920

augauugagg agcggcucaa gaccuacgcc caucuguucg acgauaaagu uaugaagcag     1980

cuuaaaagac gccacuacac cgggugggga cggcugucca gaaagaugau caauggcauu     2040

cgcgacaaac aaucuggcaa gacaauccuc gauuuucuua agagcgacgg guucuccaac     2100

agaaacuuua ugcagcugau ccaugaugac agccuuaccu ucaaagaaga gauugaaaag     2160

gcucaggugu cuggacaggg cgauucccuc cacgagcaaa ucgccgaccu ggcugggagc     2220

ccugccauua aaaagggaau ccugcagaca gucaaaauug uggaugagcu cgucaaggug     2280

augggccaua aacccgaaaa uaucguuauc gagauggcgc gggaaaacca gacgaccaca     2340

aaggggcugc agcaaucucg cgagagaaag aaacggauug aagagggcau caaggagcuu     2400

gaaucccaga uucugaaaga gaaucccgug gaaaacaccc agcuccagaa ugagaagcug     2460

uaucuguacu aucuucaaaa cggacgcgac auguacgucg aucaggaacu ggacaucaau     2520

agacucagcg acuaugaugu ggaccacauu guuccucagu ccuuuaucaa agaugacucu     2580

aucgauaaca aggugcugac acggagcguc gagaaucgcg gcaaguccga caacgugccg     2640

ucugaggaag ugguuaaaaa gaugaaaaau uacuggagac aacuccugaa cgccaaacuu     2700

auuacccagc ggaaauucga uaaucugacg aaggcugagc gcgggggacu cagcgaagcc     2760

gacaaggcug gcuuuaucaa aagacagcug gucgagaccc ggcagauuac aaagcacgug     2820

gccagaaucc uggauucccg caugaacacc aaacgggaca agaaugauaa acccauuaga     2880

gaagugaagg ucaucacacu uaagagcaaa cuggugucug acuuccgcaa ggacuuccaa     2940

cucuauaaag uucgggauau uaacaacuac caucacgccc augacgcuua ccugaaugcg     3000

guggucggga ccgcccucau caagaaauau ccuaagcugg aguccgaguu uguguacgga     3060

gauuauaagg uuuacgacgu gagaaaaaug aucgcuaaga gcgaacagga gauuggcaaa     3120

gccacggcca agcgcuucuu uuauucuaac aucaugaauu ucuuuaaaac agaagucaaa     3180

cuugcuaacg gggagauucg gaagagaccc cugaucgaga ccaauggcga aacaggagag     3240

gugguuugga acaaggaaaa ggauuucgcc accgugcgca aaguccucgc gaugccucag     3300

gugaauauug ugaagaaaac cgagguccag acaggcgggu uuuccaagga aagcauccug     3360

ucuaaacggg agagcgcuaa gcugaucccc agaaagaaag gaugggacac gcggaaguac     3420

ggcggguucg gauccccgac cguugccuau agcauucuug ugguggccaa agucgagaag     3480

ggcaaagcua agaagcugaa aucugugaag guucucgugg ggaucacaau uauggaaaaa     3540

ggcuccuacg agaaggaucc uaucggauuu cuggaagcca aaggcuacaa ggacauuaag     3600

aaagagcuca ucuucaagcu gcccaaauau agccuuuuug aacuggagaa cgggcgcaga     3660

cggaugcugg cguccgcuac cgagcuccaa aaggccaaug aacugguccu uccucagcac     3720

cuggugcgcc uccuguacua uacacagaac aucucugcca ccacgggaag caauaaccuc     3780

ggcuacauug agcagcacag agaagaguuc aaagaaaucu ucgagaagau uaucgauuuu     3840

uccgagaaau auauucugaa gaauaagguu aacucuaauc uuaaaagcuc cuucgacgaa     3900

caauuugcug ugucugauag cauccugcug uccaacagcu ucgucucucu ccugaaguac     3960

acauccuuug gggccagcgg aggcuucacc uuucuugacc uggaugugaa acaggggcgg     4020

cucagauauc agaccguuac agaggugcug gacgcuaccc ucauuuacca aucuaucaca     4080

ggccuguacg aaacgcgcac cgaccuuucc cagcugggag gcgauuga                  4128


<210>  4336
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4336
augaagaaaa uccugggccu cgaccugggg accacaagca uuggaugggc ccuugugaac       60

gaguccgaaa auaaggauga gcagucuagc aucgucagac ugggcguucg ggugauuccc      120

cucacggugg acgaaaaagg gaacuucgag aagggaaaag cuaucaccac aaaugccgau      180

cgccaacagg uccacgcggc uagaauuaac cugcagcggu acaaacuucg cagagaaaau      240

cugcgggacu gccuccagaa gcaaggcugg aucggguccg agccuaugug ugaggaugga      300

aaaucuagca ccuuugaaac auauaagcug cgcgccaaag ccgcugugga ggaaguuucc      360

cugcaugagu ucgccagagu gcucuuuaug cugaacaaga aacggggcua caagucuaau      420

cgcaaggcua acgacaaaga ggaaggcaaa cuuuucgaug ggaugaccau cgccaaaaag      480

cuguaugagg aacaccucac accggcggag uacagccugc agcuucugaa uaccggaaga      540

aaauuuacgc ccgaauauua ccgguccgac cucauugagg aguucgaaaa gaucuggaac      600

gagcagcaga aauauuaccc ugaaauucug accgaugagu uuaagcaaca gcuggaaggc      660

aaggccaaaa gcaauacauc uaagcucuuu cuggggaaau acggaaucua uuccgcugac      720

accaagggcc uugaucgcag acugcagccc cucaaauggc gggucgaggc caugaagcag      780

caaguggaca aggauguucu ggcuuuugug auuagcgacc uuaaagggca gaucgccaac      840

acaucuggac ugcucggcgc gauuuccgac cgcagcaagg agcuguacuu cucuaaacag      900

accaucggcc aauaucugug ggccucccuc caggaaaauc cucauaucag cauuaagaac      960

cagcccuuuu acagacagga uuaucuggac gaguucgaaa agaucuggga gacgcaagcu     1020

acauaccaca aggaacuuac cuccgagcug aagcaggaga uucgggauau cauuaucuuu     1080

uaucagagac cucucaaauc uaagaaaaau cugaucgccg ucugcgaacu ugagcagcgc     1140

aaggugaaag ugacagucga cgggaaggaa aaggagauua ccaucggacc gaaaauugcu     1200

cccaagagcu ccccuauguu ccaagaguuc cggaucuggc agaaccugaa caauauucuc     1260

cugcuggauc augaaaccaa cagcaaaaga ccccucuacg aggaggaacg caaucugcuu     1320

uacgaggaac ugucuguuaa ggagaaacuc uccaagacag aagcccugaa agugcugggc     1380

aaaaagagca agcaguggga ccuuaacuau aaggagcucg aagggaaucg gacgcaggcc     1440

gugcuguacg auuguuauaa cagaaucauc gucaugucug gacacgagga augcaauuuu     1500

aaaaagcuga aagcuagcga cauucgccac uacgugaaga ugaucuucaa gaaccucgcg     1560

uuuaauaccg auauucugug uuucgacucc ucucuuaaag gccaugagcu ggagcggcaa     1620

ccuaugugcc agcucuggca ucugcuguau agcuacgaag gggauaacuc cagaacagga     1680

aaugacgccc uucuccgcaa gcuggagacc agcuuuggcu uccccaaaga auaugcugcc     1740

guucuggccg acgugaucuu ugaggauucu uacggcaacc ucuccgucaa ggcuaugcgg     1800

aauauucugc cgcaccuuca ggccgggaaa gacuacagcc aggcguguga agaggcugga     1860

uauaagcacu cuagacgcuc ccugacaaag gaggaacuca accaaaaagu guacaaggag     1920

aaacuggaac ugcuuccuaa gaauagccuc cacaaccccg uuguggagaa aauccugaac     1980

cagaugguca augugauuaa cgccgucauc gaggaauaug gcaagccuga ugagauccgg     2040

auugaacugg ccagagaccu caagaucggg gcugccgagc gggaaaauau gacccgcucc     2100

auuaaccagg gaaaugcuga gaacaaaaga auccgggaga uucuggaaaa ggaguucgcc     2160

cuuucuuacc ugagcagaaa ugauaucauc aaauauaagc ucuacgagga acugaaaucc     2220

aacggcuuua agacgcugua uucugacaca uacauuagca aagauaaacu uuucuccaag     2280

gacuuugaua ucgaacauau uauccccaag gcgcgccucu ucgacgauag ccugucuaau     2340

aagaccauug aggcccggga cgugaacauc gcuaaaucca auaagaccgc cuuugauuuc     2400

auccuggaga gauacgggga acaaggcguu aaaacauaua agaccaagcu ccugcgccuu     2460

cuggaggacg gagcuauuag caacaaaaag uacaauaacc uccugaaaac agaagccgac     2520

auccccucug gcuucauuga uagagaccug cggaauacgc aguauaucgc gaagaaagcc     2580

uacgagauuc uuuccgaacu ggugaagauc gucaccccua caaccgggag cgugacaaac     2640

cgccucagag aggauuggca gcugguugac gugaugaagg agcucaacuu ugaaaaauau     2700

cagaagcugg gacuugcuga ugucaucgag gaccgggaug gccgcaaagu gaagagaauu     2760

aaagacugga ccaagcggaa ugaucaccgc caucacgcca uggacgcucu gacgaucgcc     2820

uucaccaagc cgucuuacau ucaauaucuc aacaaucuga acgccagaag cgauaaaucc     2880

agcucuaucu acgggauuga agugaaggag cugcaucggg aggaaggaaa auuucgcuuc     2940

aaugcucccc uuccuaucaa cgaguuuaga gcggaagcca agcggcaccu gaaauccguu     3000

cucgucagca uuaaggcuaa gaaaaaggug augacacaga augugaacaa aaucaagacc     3060

aaacacggca ucauuaagaa aguccagcug acacccagag gggagcucca uaaugccacc     3120

uacuauggca cgaagaagcg cccucuggug cggaugguua aagugggagc cgcucuugac     3180

gaagccacaa ucaagagagu cugcuccccc gcgauucgcg cugcccugcu caaacggcug     3240

aacgccuaca auggcgaugc uaaggaggcc uucaccggga gaaacucuau cgacaaaaau     3300

ccgauuuauc uggacaucga gcagacaaaa cuugugccug uucuggugaa gaccaucgaa     3360

uggggaaugu accaccccau ucgcaagcgg aucaccccug aucucaagau ugacaaaguc     3420

aucgauaagg gcauuagaga gaaacugaag gcgcgccucg cugaauuuaa cggggacgcc     3480

caaaaggccu ucagcaaucu ggaugagaac cccaucuaua uggacgaagc uaaacggauc     3540

acacuuaaga gaguguccau ugagggaguu cugucugcca ucccucugca uacgcucaaa     3600

gauaaggcug gcaaacccgu gauugggagc gacggcaagc cggucuuugg agauuacgug     3660

cagaccucca acaaucacca caucgccuuc uauauugacg auaagggcaa ccugcaggac     3720

aauccugugu cuuucuucga ggcggccgaa cggaaaagcc aggggguccc cguuaucgac     3780

cgcagauaca acugugauaa gggauggcau uuuguguuca caaugaaaca aaaugaguac     3840

uucguguuuc ccaacgaagc uaccggcuuc auuccuuccg aggucgaccu ucuggaugaa     3900

gccaauuauu gcaucaucag cccgaaccuc uaccgggugc agaagcuguc uaaggcuucc     3960

gggcgcgacu auugguuuag acaccaucuc gagacaaugc ugaaugauga ccuucggcug     4020

aagaacagca ccuucaaacg cauucagucu cugaagggac ucgaggauau cguuaaggug     4080

agaauuaauu ccaucggcaa aauugucgcc guggggaagu acgacuga                  4128


<210>  4337
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4337
auggagacca aguucacaua cuaucugggc cucgacaugg ggacggauag ccugggaugg       60

gccgugaccg acuccucuua ccacauccuu agagcuaaag gcaaggaucu guggggaguc      120

cggcucuuuc ccgccgcgga aacaagcguu gaccgcagaa ccuaucgggu gaaccgcaga      180

cggcgccaga gagagguggc ucggauuggg gcccugaaag aacuuuucgc cgaugagauc      240

gaaaaggucg acccuggcuu uuucgugcgc cuggaugagu ccaaauuuca uauggaggac      300

agaucugaaa auaacaagca aaaguacgga cucuucgcug auaaacuguu uagcgacgcc      360

gaguauuaca agaaauaucc gacaauuuuc caucugcgga aggaacuccu ggaguccgag      420

gaaccccacg auguucgccu uguguaccug gcucuccuga auauguuuaa acacagaggg      480

cauuuccuua acucuagccu gcagaucgag ggcgaaaauu ccaacauugc cgagagcuac      540

cagauguuug ucgacacccu caaggaugaa gaacuggacu ucccugaaca ggugucuggc      600

gaacggcugc aagagaucuu ucgcuccagc gacucuagaa agaaaaaggu ugaaaaucuc      660

cuggcggaga ugaaccuuuc caaaaagcag aaacgggaaa uccaguucgu gaaucugauu      720

ugcgggcuca agggaaaacu gagcgagcuu uuccccgaua aggaguauga cgaagaacag      780

aagaaaaucg ccuuuucuuu ccaagauucc gacuacgaag auaaggagaa cacaauucag      840

gaacugcucg acgagagcga gaugaauaug aucuaugcug ucaaagccgu gcacgauucc      900

ggcgugcugu cugggauuau gaacggagaa agauaccuga gcgucgcucg cguugccucc      960

uaugagaagc acaaaaagga ccuccagauc cugaaggaua uucuuaaaaa guacgacacc     1020

aaagaauauu uugagauguu ccggaaugag agcgaaaagu cugagaacua cuccgcguac     1080

gugggcagcg ugaaauauca ugggacguuu agacgcggac ugucuggcgg caagcaggaa     1140

gaucucuaca agcacaucaa aaagguccug gggaaauucc cucaagacga cgagggagug     1200

accuauguuc uugaagagau cgagacagag agcuuucugc ccaagcagcu caccgccucc     1260

aauggcguga uuccuaacca gguccaugcu cgggagaugc uggccauccu gaaacacgcu     1320

gaacucuacc ugccguuccu ucaagagaag gaugacacag ggcugucugu gagcgaaaag     1380

auucucaaac uguuuauguu ccagaucccc uauuacguug gaccucuggg ccaggaguau     1440

aaggauaaaa agggguacaa cgugugggcc gagagaaaag aacccggaaa gauuuacccu     1500

uggaauuuug aggaaaagau cgacgucaag aaguccgccg agaaauucau cggccgcaug     1560

gugcggagau guaccuaucu uagcggcgaa caggcucucc ccaagcaauc ucugcuguac     1620

gagaaauuuc agguccucaa cgagcugaau aaccuucgca uuagagggga aaagaucccg     1680

gugucccuga aacaggagau uuaucacgau cucuucggag acggcaagaa gguuagcaug     1740

acgcggcuga caaauuaccu gcuucagaac ggggugaucu cuuccgaaga gaaagauagc     1800

ugcauuuccg gaaucgacgg cggguuccau aauucucuca gcuccgucgg caaguuuaaa     1860

ggaguguucg gcgauaccgu ucacacagac gagcaucgca agaugaucga agauauuauc     1920

uuuuggggga ccguguucgg agacggcaaa aagcugcuga aggagaaagu cgaagaggug     1980

uaugggaacc aacucaccga agcgcagaag aaacggauuu uuggauucaa guuuucuggc     2040

ugggggagac ugagcaaagc cuuccuuaca auggagggcg cuuccaagga uaauggagag     2100

aucaagggcc ugauucaggc caugugggac acgaacugua aucucaugga acugcugagc     2160

gagcgguuua ccuacaaaga caaacuuaac gaaaugguga caaccgccga gaaaccucuc     2220

ugcgaaugga agaucgagga ucuggacgag auguaucugu cugcucccgu uaaacgcaug     2280

gucuggcaga caauuagagu gguggaugaa guccaacaga ucauggggug uccucccaag     2340

cggaucuucg uggagaugac cagauccgac ggagaaaaag gcaagcgcac gguuagccgg     2400

cagaagaaac ucguggaucu guacaaggcc cuugggaaag agggaaagau uuggucugag     2460

gagcugaaua acacacccga gggccaguuu agaaucaaaa agcucuaccu guauuaccug     2520

caaaugggga agugcaugua uaccggcgaa uccauugagc uugaaccucu caugaaugac     2580

aaccaccugu acgauaucga ccauauuuau ccgcgccacu ucaucaaaga ugacaacauc     2640

agcaauaacc ugguccucgu gaagaaagag agaaaugcgc acaagucuga uagcuuuccc     2700

auugagccug aaauccggaa caaaaugaug cccuucugga agucccugcg caagaaagga     2760

uuccuuaccg aggaaaagua caauagacug acacggauga gcgcuuuuac cgacgaggaa     2820

aaagccgccu ucauuucucg ccagcucguu gagacauccc agggcacgaa ggcuaucacc     2880

caaauuuuua aacaggccuu cgcugauagc gagaucgugu uuuccaaggc cucugucgug     2940

agcgcguuca gacagaaguu ugacuguuau aaaguucggu gcgugaacga cuaccaucac     3000

gcccaugaug cuuaccugaa uaucgucgcc gggaacacau aucgcgugaa guucaccucc     3060

aauccucuga acuuuauuaa agaagcuaag aaaaauccca acucuagaga gaaucgguac     3120

aacauggaca agacauucga augggaugug acccgcaaug gagagacggc cugggucgcg     3180

cagagcaacg aaaacccggg caccauccaa cuuguuaaga aauaucugaa uaagaacucc     3240

ccucucauua caaaaauggu guacgagaag cacgggggaa ucacccagaa agccacaauu     3300

uggucugcua agaaagccga gagcggcaau accgacgcuu auauccccgu gaagaugaac     3360

gauccuagac uggccgacgu cacgaaguac gggggcguga caaccaucgc cguugcugga     3420

uauacacucc uggaauacaa agcgggcggg aagaccgugc ggucccuuga ggcccugccc     3480

auuuaccucg gaggcagcaa aaaucugacc gaggagcaga uggucgaaua uuuuucuaac     3540

auccugcagc aagagaauaa ggagaaagug guuuccgaug ugauuaucag aaagaagaug     3600

auuccuagcg ggucucuuau caaauacaac ggauucuauu acuaucuggc uggcaagaca     3660

ggcaaucgca uuguccucau cuccgccgug cagcuguguc uccccaugga cacgaugcug     3720

uacaucaaaa agauugaaaa agccugcagc accgggaacu ucgaggaaaa ggauaagaau     3780

ggaaaacagg uucuuucuaa gcagcaaaac gaguuugugu augagcugau cauugaaaaa     3840

uaccagcagu ccaucuucaa gaaaaagguc ggcgcuauug gggacacaau caucgagaag     3900

cgggaaaaau uugccgagau uagccucgag gaacaaugua aggugcugau gcagaucuuc     3960

cugaauuuuc aguccggaau uggcguggau cuuaccagcg ucgacggguc uaacaaaagc     4020

ggaguuacau accugaauaa gaaaaucaug aacgcgcagg agcucaagcu gauugaccaa     4080

uccgugaccg gcguguucuc uagagaaauc gaucuccuga cgaucuga                  4128


<210>  4338
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4338
augagcaaca aguccuacuc uaucggccug gacauuggga ccaauagcgu gggaugggcc       60

gucaucacag augacuauaa aguucccucc aagaaaauga aggugcucgg caacacggau      120

aaacacuuca uuaagaagaa ucugaucggg gcccuucugu uugacgaggg aaccacagcu      180

gaagauagac ggcucaaacg caccgcgaga cggcgcuaca caagacggaa gaaccgccug      240

agauaucuuc aggagauuuu cucugaagag augagcaaag uggacuccag cuuuuuccau      300

cggcuggaug acucuuuucu cgucccugaa gauaagcgcg gcuccaaaua cccgaucuuc      360

gcuacccugg ccgaggagaa ggaauaucac aagaaauuuc ccacaaucua ccaucugaga      420

aagcaccucg ccgacagcaa agagaagacc gaucugcggc uuauuuaucu ggcucucgcc      480

cauaugauca aauaccgcgg gcacuuccug uacgaagagu cuuuugacau uaagaauaac      540

gauauccaaa agauuuucuc cgaguuuauc agcauuuaug acaauacguu cgaagagucu      600

ucccuuaucg gacagaacgc ucagguggaa gccaucuuua ccgacaagau uagcaagucu      660

gcgaaaagag agcggguucu gaaacucuuc ccugaucaga aauccacagg ccuguucagc      720

gaauuucuga agcucauccu gggcaaucaa gccgacuuca aaaagcacuu ugaucuugag      780

gagaaggcuc cccugcaguu cuccaaagac accuacgaug aagaccucga gaaccugcuu      840

gggcagauuc gcgaugacuu ugccgaucug uuccucgugg cuaagaaacu guaugacgcc      900

auccugcucu cuggcauucu gacagucaag gaucuuagca ccaaagcgcc ucuguccgcc      960

agcaugaucg aacauuacga gaaucaccag aaggaccucg cugcccugaa gcaauuuauc     1020

cagaacaauc uucaggaaaa auaugacgag guguucucug aucaguccaa ggacggauac     1080

gcuggguaua uuaacggaaa aacgacacaa gaggccuuuu acaaguacau caaaaaucug     1140

cucagcaagu ucgaaggcgc cgauuauuuu cuggacaaga uugagagaga agauuuccug     1200

cggaaacaga gaaccuuuga caacgggucu aucccccauc agauucaccu ccaagagaug     1260

aacgcuaucc ugcaccgcca gggagaacau uacccuuucc uuaaggagaa uaaagagaag     1320

aucgagaaaa uucugaucuu ccggauuccg uauuacguug gcccccucgc gagaggcaac     1380

cgcgauuuug ccuggcugac acggaauagc gacgaagcua ucagaccuug gaacuucgaa     1440

gagauugugg auaaggccuc cucugccgag gacuuuauca auaaaaugac caacuaugau     1500

cuguaccuuc ccgaagagaa gguccucccu aagcacagcc ugcuguauga aaccuucgcu     1560

guguacaaug agcucacaaa agugaaguuu auugccgaag ggcugacgcg cuaccaguuc     1620

cuugacaaaa agcagaaaaa ggauaucuuu uauaccuucu uuaaggcgga ggacaaacgg     1680

aaggucacag agaaagacau cauucauuac cugcacaccg uugauggaua ugacggcauc     1740

gaacucgagg ggauugaaaa gcaauucaac gcuucccuga gcacauacca ugaucugcuu     1800

aaaaucauua aggacaagga guuuauggau gacgauaaaa augaagccau ccucgagaac     1860

aucgugcaca cccugacgau uuucgaggac agagaaauga ucaagcagcg ccuggcccag     1920

uauaauucuc ucuuugauga gaaagugauu aaggcucuga cacggagaca cuacaccgga     1980

uggggcaaac uuuccgccaa gcugaucaac gggauucgcg acaagaaaag cggaaagacc     2040

auccucgauu aucugaucga cgauggcgaa auuaaucgga acuucaugca gcugauccau     2100

gacgacggcc uuucuuucaa agagauuauc caaaaggcuc aggucuuugg gaaaacaaau     2160

gaugugaagc agguugugca cgaacucccc ggauccccgg ccauuaaaaa gggcauccug     2220

cagagcauca agauugucga cgagcuggug aaaguuaugg ggcaugcgcc ugaguccauc     2280

gugauugaaa uggccagaga gaaccaaacc acagcucggg gaaagaaaaa cucucagcag     2340

cgcuacaaga gaaucgaaga uagccucaaa aaucuggccu ccggccuuga cucuaacauu     2400

cugaaggaga aucccacgga uaacguccag cuccaaaaug accggcuguu ccuguacuau     2460

cuucagaacg ggagagauau guacaccggc aagccucucg agaucaauca gcugagcaac     2520

uaugacauug aucacaucau cccccaggcu uuuauuaaag acgauucccu ggacaaucgc     2580

gugcucacaa gcucuaagga caaccgggga aaauccgaua auguccccag ccuggaagug     2640

guugagaaga ugaaagccuu cuggcaacag cuucuggacu cuaagcucau cuccgaaaga     2700

aaguuuaaca aucugaccaa agcggagcgc gaaagaggcg ggcugaacga gcuugauaag     2760

gugggauuca uuaaacggca gcuggucgag acacgccaaa ucaccaagca cguggcccag     2820

auucucgaca cgagauuuaa uaaagaaguu accgagaagg auaagaaaaa ccggaacgug     2880

aagaucauca cacugaaaag caaucucguc ucuaacuucc gcaaggaauu uggccuguac     2940

aaagugagag agauuaauga cuaucaucac gcucaugaug ccuaccuuaa cgcugugguu     3000

gccaaggcca uccugaagaa auauccuaag cucgaaccgg aguucgucua cggggaguac     3060

cagaaauaug accugaagcg guacauuagc cgcuccaaag aucccaagga caucgaaaaa     3120

gcuaccgaga aguacuuuuu cuauagcaau cugcuuaacu ucuuuaagga agaggugcag     3180

uaugcggaug gaacaauugc caaaagagaa aauaucgagu acucuaagga caccggcgag     3240

aucgcuugga acaaagaaaa ggauuucgcc acggugaaaa agguccuguc ccucccucaa     3300

gugaauauug uuaagaaacg ggaggugcag acagggggcu uuagcaagga auccauccug     3360

cccaaaggaa acucugacaa gcucauuccu cgcaaaacca aggacauccu gugggagaca     3420

accaaguaug gcggguucga uagccccguc auugccuacu ccauccuucu gauugcugac     3480

aucgagaaag gaaagucuaa agaacucaag accgugaaaa cacugguugg caucacgauu     3540

auggagaagg ccaccuuuga agagaauccg aucacauucc uggaaaacaa gggguaccac     3600

aaugugagaa aagagaacau ucuuugccug ccuaaguaua gccucuuuga gcuggaaaau     3660

ggacggagac gccuccuggc guccgcuaaa gagcuucaga agggcaacga aaucguccug     3720

cccguguacc ugaccacacu ccuguaucau ucuaaaaacc uucacaagcu ggaugagccu     3780

gggcaccucg aauacauuca gaaacaucgg aaugaguuca aggaccugcu caaccugguu     3840

agcgaguuuu cccaaaagua uauccuugcc gaugccaauc uggagaaaau ccagaaccug     3900

uacgcugaca augaacaggc cgauauugaa auccucgcga acagcuucau uaaucugcuu     3960

accuuuacga cacugggcgc ucccgccgcc uucaaguuuu ucggaaaaga caucgauaga     4020

aagcgcuaua ccaccguguc ugagauucuc aacgcuacac ugauccacca auccaucacc     4080

ggccucuacg aaacacggau ugaccugagc aaacuugggg aggauuga                  4128


<210>  4339
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4339
auggaaaaga gcuacuccau cggccuggac auugggacca acucuguggg augggccguc       60

aucacagaug acuauaaagu ucccgcuaag aaaaugaagg ugcucggcaa uacggauaaa      120

aaguacauua agaaaaaccu gcuuggggcc cuccuguucg acagcggaga aaccgcggag      180

gugacaagac ugaagcggac cgcucgcaga cgguauacac gcagaaagaa ucggcuucgc      240

uaccugcagg aaaucuuugc caaggagaug accaaagucg augaauccuu cuuucaaaga      300

cucgaggagu cuuuccugac agacgaugac aagaccuuug auagccaccc uauuuucggc      360

aacaaggccg aagaggacgc uuaucaucag aaauuuccga cgaucuacca ucugcggaag      420

uaucucgccg auucccagga aaaagcugac cugcgccuug uguaccuggc ccucgcgcac      480

augaucaagu acagagggca cuuccugauu gagggagagc uuaaugccga aaacaccgau      540

guucagaaac uguuuaaugu guucgucgag acauaugaca agaucgugga cgaaucucau      600

cucagcgaga uugaaguuga ugcuuccagc auccugaccg agaaaguguc uaagucccgg      660

cgccuggaga accucauuaa gcaauacccc acagaaaaga agaauacccu guuuggcaac      720

cuuaucgccc uggcucucgg ccugcagccu aauuucaaaa cgaacuuuaa acuuagcgag      780

gacgccaaac ugcaguucuc uaaggauaca uaugaagagg accucgagga gcugcugggg      840

aaggucggag augacuacgc ggaucucuuc auuuccgcca aaaaucugua ugacgcuauc      900

cuucugagcg gcauccucac cguggaugac aacucuacaa aggcgccccu guccgcuagc      960

augauuaaaa gauacgugga gcaccacgaa gaucuugaga agcugaaaga auuuaucaag     1020

auuaauaagc ucaaacugua ucaugacauc uucaaggaca aaaccaagaa cggguacgcc     1080

ggauacauug auaacggcgu caaacaggac gaguuuuaua aguaccugaa gaccauccuc     1140

acaaaaaucg augacuccga uuauuuccug gacaagauug aacgggauga cuuucuucgc     1200

aaacaaagaa cguucgauaa ugggucuauc ccucaccaga uucaucugca ggagaugcac     1260

agcauccucc ggagacaggg agacuacuau cccuuucuga aggagaacca agccaaaauu     1320

gaaaagaucc ugaccuuccg caucccuuac uauguuggcc cgcuugcucg gaaagauucc     1380

agauuugcgu gggccaauua ccacagcgac gagcccauua caccuuggaa cuucgaugaa     1440

gugguggaca aggagaaguc ugcugagaaa uuuaucaccc gcaugacacu caaugaccug     1500

uaccugcccg aagagaaggu ccucccuaaa cauucccacg uguaugaaac cuucacgguu     1560

uacaacgagc ugacaaagau uaaauaugug aaugaacagg gcgagagcuu uuucuucgau     1620

gccaacauga agcaggagau cuuugaccau gucuucaagg aaaaucggaa agugaccaag     1680

gccaaacuuc ugucuuaccu caacaaugag uuugaagagu ucagaauuaa cgaucugauc     1740

gggcuggaca aggauagcaa auccuuuaau gcuucucuug gaaccuauca cgaccucaag     1800

aagauucugg auaagagcuu ccuggacgau aagacaaacg gccaaaucau cgaagacauu     1860

guucucaccc ugacacuuuu ugaggaucgc gacaugaucc augagcggcu gcagaaauac     1920

uccgauuucu uuacgagcca gcagcucaag aaacuggaaa gacgccacua uaccgggugg     1980

ggacggcugu cuuacaaacu uauuaauggc aucagaaaca aggagaacaa uaaaacaauu     2040

cucgacuucc ugaucgacga ugggcacgcc aaccgcaauu uuaugcaacu gaucaacgac     2100

gaaucccuca gcuucaagac cauuauccag gaggcccagg uggucggaga uguggacgau     2160

auugaagcug ucgugcauga ccugcccggc ucuccggcca ucaaaaaggg cauucuucag     2220

uccguuaaaa ucguggauga gcuggucaag gugauggggg acaauccuga uaacaucguu     2280

auugagaugg cccgggaaaa ucaaacaacc ggauacggca gaaacaaaag caaucagcgg     2340

cucaagcgcc ugcaggacuc ccugaaggag uuugggucug auauccuuag caaaaagaaa     2400

cccuccuaug uggacucuaa ggucgaaaac agccaccucc agaaugacag acuguuccug     2460

uacuauauuc aaaacggaaa agauauguac acgggcgagg agcucgacau cgaucggcug     2520

uccgacuaug auauugacca uaucaucccu caggcuuuca uuaaggauaa uagcaucgac     2580

aacaaggugc uuaccucuuc cgccaaaaau agaggcaaga gcgaugacgu gcccucuauu     2640

gaaaucguuc gcaaccggag auccuacugg uauaaacugu acaagagcgg gcucauuucu     2700

aaacgcaagu uugauaaccu gacaaaggcu gagagaggag gccugaccga agccgacaaa     2760

gcgggguuca ucaagcggca gcuugucgag acacgccaga uuaccaaaca cguggcccaa     2820

auccuggaug cuagauuuaa uacgaagcgg gacgaaaacg acaaagugau ccgcgauguc     2880

aaggugauua cacucaagag caaucugguu ucccaguuca gaaaagaguu uaaguucuac     2940

aaagugcggg agaucaacga cuaucaccau gcccacgaug cuuaccucaa ugccgucgug     3000

ggaaccgcgc ugcuuaagaa auaucccaag cugacaccug aauuuguuua cggcgaguau     3060

aagaaauacg acgugcgcaa gcucauugcc aaaagcucug augacuauuc cgaaaugggg     3120

aaggcuaccg ccaaauacuu cuuuuacagc aaccugauga auuucuuuaa gaccgagguc     3180

aaauaugcug auggaagagu guucgaacgg ccggacaucg agacaaacgc cgauggcgag     3240

guugugugga auaagcagaa ggacuucgau auuguccgca aagugcuguc cuacccccaa     3300

gugaacaucg ucaagaaagu ugaagcccag acagggggcu uuucuaagga gagcauccuu     3360

uccaaaggag acucugauaa gcugauuccu agaaagacca aaaaggugua uuggaauacg     3420

aaaaaguacg gcggguucga cagccccacc guggcuuauu ccguccucgu gguugcggac     3480

aucgaaaaag gaaaggccaa gaaacugaag acagugaaag agcucgucgg cauuucuauc     3540

auggaacgga gcuuuuucga ggagaacccu guguccuuuc uggaaaagaa aggguaccau     3600

aauguucagg aggauaaacu uauuaagcug cccaaauaua gccucuucga auuugaggga     3660

ggcagacgcc ggcugcuggc uucugccacc gagcuucaga aggggaacga agugaugcug     3720

ccggcccacc ucgucgagcu gcucuaccau gcucacagaa ucgacuccuu caauagcacg     3780

gaacaccuga aauacguguc ugagcauaag aaagaauuug agaagguucu uuccugcgug     3840

gagaacuuca gcaaccugua ugucgaugug gagaaaaauc ugucuaaggu gcgcgccgcg     3900

gcugaaucca ugacaaacuu uagccucgaa gagaucuccg ccagcuucau uaaucugcuu     3960

acccugaccg cccucggcgc uccugccgac uuuaacuucc ugggagaaaa gaucccccgg     4020

aaaagauaca caucuaccaa ggagugucuc agcgcgacac ugauucacca auccgucacg     4080

ggccuuuaug agacccgcau cgaucugucu aaacuggggg aagaguga                  4128


<210>  4340
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4340
augaccaaga ucaaagacga uuacauugug ggccuggaca ucgggacaga uagcugcgga       60

ugggucgcca ugaacuccaa uaacgacauu cucaagcugc agggcaaaac ggcuaucggg      120

ucuagacuuu ucgagggagg caagagcgcc gcggaacggc gccuguuuag aaccacacac      180

cggcgcauua aaagacggcg cuggagacuc aagcugcuug aggaguucuu ugaucccuau      240

auggcugagg uugacccuua cuucuuugcc cggcugaagg aauccgggcu cucuccgcug      300

gauaaacgca agaccgugag cuccaucgug uuccccacau cugccgagga caaaaaguuu      360

uaugaugacu acccuaccau cuaucaucug agauacaaac ucaugacaga ggaugaaaag      420

uucgaccugc gggaggucua ucuugcuauu caccauauca uuaaguaccg cggaaauuuu      480

cuguacaaca ccagcgugaa agauuucaag gccuccaaaa ucgacguuaa gagcucuauu      540

gaaaaacuca augagcugua ugagaaccuu ggccuggacc ucaaugugga auuuaacauc      600

uccaauacgg cugagauuga aaagguccug aaagauaagc aaaucuucaa gagagacaaa      660

gugaagaaaa ucgccgagcu guuugcgauu aagaccgaua acaaagaaca gagcaagcgg      720

aucaaggaca uuucuaaaca gguugccaau gcugugcucg gcuacaagac acgcuucgau      780

accaucgccc ugaaagagau uuccaaggac gagcuuagcg auuggaacuu caaacugucu      840

gacaucgaug cugacuccaa guuugaagcc cucaugggga aucuggauga gaacgaacag      900

gcgauccuuc ugacaauuaa ggaacucuuc aaugaaguca cccugaacgg aaucguggag      960

gacggcaaca cgcugagcga guccaugauu aauaaauaua acgaccacag agaugaccuc     1020

aagcugcuua aagaagugau cgagaaucau auugaucgga agaaagccaa ggaacuggcu     1080

cucgccuacg accuguaugu caacaauaga cacgggcaac uucugcaggc uaagaaaaag     1140

cucggaaaaa ucaagccccg cucuaaagag gauuuuuaca agguugugaa caaaaaucug     1200

gacgauagcc gggccuccaa ggaaaucaag aaaaagauug agcuggacag cuucaugccu     1260

aaacagagaa caaacgccaa uggcgugauc cccuaucagc uccaacagcu ggagcuugau     1320

aagauuaucg aaaaccaguc uaaauacuac ccuuuucuga aggagauuaa uccggucucc     1380

agccaccuca aggaagcucc cuauaaacug gacgagcuga uccgcuuccg ggugccuuac     1440

uauguugggc cccuuauuuc uccuaacgag agcaccaagg auauccagac caaaaagaau     1500

caaaacuuug cguggaugau cagaaaagaa gagggacgca uuacacccug gaauuucgac     1560

cagaaggugg aucggaucga auccgccaac aaguuuauua aaagaaugac cacaaaggac     1620

acguaccucu ucggcgagga cguccugccg gcuaacucuc ugcucuauca gaaauuuacc     1680

gugcugaaug aacuuaacaa uauccgcauu aacggcaagc ggaucagcgu ugaucugaaa     1740

caagagaucu acgagaaucu cuucaagaag cauacaaccg ugacagucaa aaagcuggaa     1800

aacuaucuga aagagaauca caaccuugug aaggucgaaa uuaaagggcu cgccgacgag     1860

aagaaauuua auuccggacu gaccacguac aacagauuca agaaucugaa caucuucgau     1920

aaucagauug acgaucucaa guaccgcaac gacuuugaga aaaucauuga guggagcaca     1980

aucuucgagg auaagucuau cuauaaagaa aagcugcggu ccauugacug gcuuaaugag     2040

aaacagauca acgcccugag caacauuaga cuccagggcu gggggcggcu gucuaagaag     2100

cugcuugcuc aacuccauga ucacaaugga cagaccauca uugaacagcu gugggacucc     2160

cagaacaauu uuaugcaaau cgugacccag gccgauuuca aagacgcgau cgcuaaggcc     2220

aaccagaauc ugcucguugc cacaagcgug gaggauauuc ugaacaaugc uuacaccucc     2280

ccugccaaca aaaaggcuau ccgccagguc auuaaagugg uugacgacau cgugaaggcc     2340

gcgucuggca agguccccaa acaaauugcc aucgaauuua caagagaugc ugacgagaau     2400

ccuaagcgga gccagacgag aggguccaaa cuucagaagg uguauaaaga ucugucuacc     2460

gagcucgcca gcaagacaau ugcugaggaa cugaacgaag ccaucaagga caaaaagcug     2520

gugcaggaua aauacuaucu uuacuucaug caacucggac gcgacgcgua uaccggcgag     2580

cccaucaaua uugaugagau ccagaaguac gacauugauc acauccugcc ccaguccuuu     2640

auuaaagacg augcccugga caaccggguu cucgucagca gagcugugaa uaacggcaag     2700

ucugacaaug ugccugucaa gcuguucggg aacgaaaugg ccgcuaaccu uggaaugaca     2760

auccgcaaaa ugugggagga auggaagaau aucggccuga uuuccaaaac caaguacaac     2820

aaucuccuga cggauccgga ccauaucaac aaauauaaga gcgccggguu uauuagacgg     2880

caacuggugg agaccucuca gaucauuaaa cuuguuucca caauccugca gagccgcuac     2940

cccaauaccg aaaucauuac agugaaggcc aaguauaacc acuaccucag agagaaauuc     3000

gaucuguaua agucucggga ggucaaugac uaccaucacg cuaucgaugc guaucucagc     3060

gccauuugug gaaaccugcu uuaccagaau uacccuaacc ugcgccccuu uuucguguau     3120

ggccaauaca aaaaguuuuc cagcgacccu gauaaagaaa aggcuaucuu caauaagacc     3180

agaaaauucu cuuuuauuuc ccagcuccug aagaacaaaa gcgaaaauuc caaggaaauc     3240

gccaaaaagc ugaagcgggc cuaucaguuc aaauacaugc uuguuucucg cgagacggaa     3300

acaagagacc aggagauguu uaagaugacc guguaucccc gguucagcca ugauacaguc     3360

aaagcuccgc gcaaccugau uccuaagaaa auggggaugu cccccgacau cuacggcgga     3420

uauaccaaua acucugaugc cuacauggug aucguuagaa uugacaagaa gaaaggcacc     3480

gaguacaaga uccucgggau uccuacacgg gaacugguga aucucaaaaa ggcggagaaa     3540

gaagaucacu auaagagcua ccugaaagag auccuuacgc cccgcauucu guauaacaag     3600

aacggaaaga gagacaaaaa gaucaccucc uuugaaaucg ucaaaucuaa gauuccuuac     3660

aaacaaguga uccaggacgg cgauaagaag uucaugcucg ggagcuccac auauguguac     3720

aaugcuaaac agcugacccu gagcacagag ucuaugaagg ccauuaccaa caauuuugac     3780

aaagauuccg acgagaacga ugcccuuauc aaggcuuaug acgaaauucu ggauaaaguc     3840

gacaaguacc ucccccuguu cgauaucaau aaguuucggg agaaacucca cagcggaaga     3900

gaaaaguuca ucaaacuguc ucuugaggac aagaaagaua cgauucugaa gguucuggag     3960

ggccuccaug acaacgccgu gaugacaaag aucccgacca uugggcuguc caccccucuu     4020

ggcuuuaugc aguuccccaa uggagugauc cugagcgaaa acgcgaaacu cauuuaccaa     4080

ucucccacag gccuguucaa gaaauccguc aagaucagcg aucucuga                  4128


<210>  4341
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4341
augagcaacu ucgacaauca caaggauucc aucugcaaac auaagggccu gaaaaagggg       60

aacuacuaua uuggacucga caucggcacc aauucugugg ggugggccgc uacaaacgag      120

caguacgaac ugcuuaaauu uaagagccac aagauguggg gauccagacu guucgccgag      180

gcgcaaacgg cugucgauac ccggaugaaa cgcucugcca gacggcgccu ccagagacgg      240

aaguuucgcc ugauucuucu ggaaagccuc uucuccaaag agaucaaugc cguugacgaa      300

acauuuuuca ugagacugca ugagucuaac uaucacgugg aggauaagac caaucuggug      360

aaauacacac ucuuuauuga caagaacuau accgaugucg acuacuauaa ggaguucccc      420

acaaucuacc aucugcggca ccaccuuaug accgagggca cgaaagauau ccgccugcuc      480

uuucuggcua uucaucacau ccuuaaguac agagggaauu uccuguaugg gaaaaaggaa      540

uuuagcgugg acggcgagau guacuccguu cuccggagca cccuggagga acugggcuuc      600

gggucuuaug uggaggauga aaacguccuc caguccauug ccaaucuggu gcuugaccgc      660

ggaaucgcua gaagcgagaa aguuaagcag auugaaccuc ugaucgccca aggcaaaaag      720

cucucuuccg uggagaagaa ccggcugaaa gcgugggccu accuuauuau ggggcugaag      780

gcuaaucucg ucaaccuguu uacagaugag gacguggaag ugguccugcc ggaggaaaaa      840

gaguucaaga gcaucaaucu ccaugaagga aucuaugagg acguucgcga ugccuacgag      900

gacgcuuggg gcgauagauu cgccauugug gaccagugua aagugcugca cgauaucauu      960

gucaucucuu ccauuaucaa gcccgggcag agccuuuccg aagcgaagau cgagucuuau     1020

aaccagcaca aagaagaccu ggugcuccuu aagagcguuc uuaaagagga uaagaaacug     1080

uacgacgaua uguuuguggu cgacauugau aagggaacca acuacgugaa guauaucaaa     1140

caaggcguua auggcaacac auccaccacg caggaagccu ucuacaagua ugugcggaaa     1200

gucgugagcg uccucccuga gacagagaau accagaugca uugugcagaa gcuggaagag     1260

gggacauuuc ugccccucca gcgcaucucu aaaaacggag uugugccuua ccaacugcau     1320

aaggaggaac uuguccggau ucuggagaag gcuaaacuca auuuccccuu ucugaacgaa     1380

guggaggacg gccugaccgu ugccgacaag aucaucucca uucuugaguu caaaaucccu     1440

uauuacgugg ggccgcucaa ucccacccac agcauugaaa acggaggcuu ugcuuggguc     1500

gugagaaagg aagccggaca ggugcugccu uggaauuucg agcagaaagu ugauguccaa     1560

gccucugcug ugaaguuuau cgagaaccug acaaauaaau guacguaucu cauugaccag     1620

gaugugcugc ccaaguacag ccuucuguac gcggaguucu cccuccugaa cgaacugaau     1680

aacauccgcg ucaaugacaa gccucuuccc gccgauguga aacaggcuuu uauucgggac     1740

cauuucaucu cucuccagga uagcaagacc gccaccaaaa agguuguggc caaauaucug     1800

gucgacaaca acuacguguc ccaaccgccu aucauuacag gaauggauca gcagaucgcu     1860

acaaagcuga ccagccacag agacaugacg cgcauucucg gcgaacauuu ugauuaugcc     1920

auggcggagg acaucauucg guacaucaca aucuucggcg aaucuaagga uaugcugaga     1980

cagguucuua aaaauuauua cggggaccgc cugaccaccg agcaaauuaa gaaacucgga     2040

aagcugacau auaccggcug ggggcggcug uccaaaaagu uccuuagcga caucugggga     2100

augaagaaaa acgaugacga ugcugaggcc ggcacaauua ucgaacacau gagagagggg     2160

caggaaaauc ucaugcagcu gcugucuucc agcuacuccu uuauugacgu gguccaggag     2220

uacgugacga acguucucgg cgccaaggag aaaaccgcuu ucgaaaugcu ggaggaucuu     2280

ucucugagcc ccauggugaa gcgcuccguc uggcaaacac uccggauccu ggacgaaauc     2340

gugucuauua gaaaagagcu gccuaagaag aucuuugugg aagucgcccg gaccaauaaa     2400

gcugauaaga aacgcacaca gagcagaaag gcccuucucg aggagcugua ugaacagauu     2460

aaagacgagg auguucacgu gcugcagcaa gaacucgagu ccaaggaaga cagcgcgcug     2520

aacucuaaaa agcuguaccu guauuucacc cagaugggac ggugcaugua cuccggcaga     2580

cgcaucgaua uucaugagcu cuuuacggac accuaugaua ucgaccacau cuacccccgg     2640

agccugacaa aggacgauuc uuggcauaau auugugcugg ucgagaaaca gcuuaacgcc     2700

gaaaagaccg acagauuccc cauccugucc gcuauucaaa gcaaacagaa gggguuuugg     2760

aaacuccugu gggaucgcgg auucaucucu gagaagaagu augacagacu cgugcggaca     2820

accgaucuga cggacgagga acuuaaucag uuuauuaacc gccagaucgu uagcacaaau     2880

caauccguga aagccgucac cacacugcuc caccagcugu acccugaaac cgaggugguu     2940

uucagcaagg cugagaacau uucugauuuu agacgggacu acggcuucgu gaaaguccgc     3000

uccaucaauc accaucacca ugccaaggau gcguaucuga acaucguggu ugggaaugug     3060

uaccacgaaa aauuuaccaa gaacuuccuu agcuucgcca agcagaaagg aacacauaga     3120

acguauaacc ugaccaagau guuugaguac gaaauuaaac ucacagagga auccaagaaa     3180

gucaucuggg acccgaauau ucacauggau aucgugaaga agaugaugaa aucuaacgac     3240

gugcggguca cccagcgcgc uguuccccaa aagggcgggc ugagcgacga gacaauuuau     3300

aaaaagggca ucgccaaaag aggaguguac cucccucuga aguccucuga ugagcgggug     3360

cgcgacgucg aaaaguaugg cgggagaacc agcaucacga uuuccgagua caucauucuu     3420

aauauccggg auacaucuga ccaggauaga auugaaaucu ucagccugcc ccucuacauc     3480

gcuggcaaaa aggacauuca caaagccauc gaggaggccu aucuguccac cuguaagaaa     3540

ccugcuagcg ugguugauau ucagauccug uaccgcggac uuuauauugg gucuaaggug     3600

aacaucaaug gauuuaccua cuauaugggc gggaaaacag gcuccaacgu cagcguggac     3660

ucugcgguug auguggucau ggacauuacc uccagcgugu acaucaagcu gcucgaaaag     3720

gucgccauca aaaagauuga aaauucuaaa acauuccgga ucgaaaacau uaagacgucc     3780

uauaaucaua aagaggugac cguuagcaag gaacagaaca ucgcucugua ccaagagcuc     3840

cugaagaaac uugauacacc ccuguuuucc aagaugaaag gaaauaagcu ggaggaguuc     3900

agaaccgugg gcuuugacac auucauugcc cucagccugg aagaucaggc cucuguccuu     3960

cuggaagugc ucaacguucu gaccaauuuu aaaacgaagc gcgacaucac caagaucggg     4020

augacaagcu cccggucuac ccucggaauu aacaucagca auacagagca guucuccgug     4080

auuaccacau cuaucaccgg ccuguacgaa caagagauua aaaucaagug a              4131


<210>  4342
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4342
augaccacaa agaacagaga gaaaaauaac aaguuccaca aagccagccc ccuguacacg       60

cucggccugg acaucgggac cacauccgug ggauuuaccg cuauugauga agcccagcgg      120

ccuaugcgcg cgaagggcaa aacagucauc gggguuagac uuuuccaaga gggacagucu      180

gcugugggcc ggaagaauuu ucgcaccaca agacggcgcc ugaagagacg gcgcagacgg      240

cucaaccugc uuaaacaguu cuuuagcgcc ccgcugcgca agauugaccc caccuucuuu      300

aaaagacuca aggccuccuc ucugagcccu gcugauaaac ggcgcuaucu ggcccccggg      360

cuguucccua auggaggcga caucaaguuu uaccagaagu aucccacgau uuaccaucug      420

agacuugcuc ugaugaccga aaaacggccu uucgaucuca gagaggugua ucuggccauc      480

caccauaucg ucaaguaccg cggcaacuuu cuuaauccgc ugcccguguc cgcguuccgg      540

gggagaaacc aacgcuuuga agaccaguuc ucugagcuca aucagcugua uaaaagccug      600

gagggagaau ccccuuucca guuugaugag aagauuuuca gcgaacucga caaacaacug      660

cuuguuaacg auggccggaa gaucgacaga cagaaacagc uggccaagcg cguguuuauu      720

aagacaaaug agaaacggau cgauucucac caucagaaga uugcuuccgc cuucagcaaa      780

gcugucuuug gguacaaguu cagacccgac gugcuccuga acucugaucc uaaugagucc      840

aucgacgaau uuauuaaauu cggagacgag gauuuugacg auaaacuugu uauccugcgc      900

gaaagccuca acgccaccca aaaggagauc cuggcgguga cacagcggau uuacucucag      960

aucauucuga augaaaucgu ccccuccaga gagacccuca gcgaggccau ggugucccgc     1020

uaucaaaaac accacucuga ccugcagcuu cugacggagg cuugcaagaa aaugacaaag     1080

gccaaagcug cccggauuca ggccgcuuac accgauuaua ucggcaaggg ggacggacau     1140

aagcucgugc acgaucaguu cgucgcgcug aucaaaaaga accuugaccc gagcgaagaa     1200

ggcagagauc uggccgagcg gcucgcucaa gacgauuuua ugccucugca gcgcacaccc     1260

cugaacagag uuauuccuca ucagcuccag caagaggaac uggaccggau cauugagaau     1320

cagggcgaau acuaucccuu ccuuaaaacc cugaacccca auagaucccg cgccggggug     1380

gccaacuacc agcucgacga gcuggugagc uuucggaucc cuuauuacgu cggaccgcug     1440

auuaccgcua cagaucagcu ucacacgucu ggcgccaagu ucgcguggau gaccagacgc     1500

gcugccgggg aaaucacacc cuggaauuuc gaccaaaaag uggauagacu cgccuccgcu     1560

aaccaguuua uccggcagaa uaugccuaag gacaaccauc ugcuggaggc cgauguucuc     1620

cccgcuaaua gccugaccua ccagcgcuau cuugugcugg acgaacucaa cgccgucaag     1680

gugaaucaca aaucucugac aguuagccaa aagcagagaa uuuucaacga ucuguuuaaa     1740

cggcagcgca ccgugacggu ccuuaaucuc caaaacuacc ugaaguccau cgagaaacug     1800

ccuacacucc ccgugauuac cggagucucu gacagcgaga gauucaagac cacacugucc     1860

accuaucuug auuuuaaaaa uaucuucggc gggaaggugg acgauagcuc ucagaaggaa     1920

aaauuugaga agauuauccu guacgcgaca auuuucgaag accacgccgu uuauaaagcu     1980

aagcucgauc agaucgacug gcugggacag caacagaaaa cggaccugcu uuccaagaac     2040

uaccagggcu ggggccagcu cagcgagaag cugcugcucg ggcugaaaaa ccaagccgga     2100

cagucuaucc uugaugagcu guggcagacc aauaagcggu uucgcgaaau uaugaacgag     2160

gcucauuucg ccaaacagau cauggcgucc aaugccgcuc uccugagaaa gaaaggccug     2220

gacgaugugc uuagcacagc cuuucucucc ccgcaaaaca agaaggcuau ucggcagacc     2280

gucgaagugg uugacgauau cgugaaagcc auuggguguc cuccccagaa gaucucucug     2340

cacuucagca aagccccugc ugacgcgauc cgccaugaga gagauaaugc caacauuauc     2400

cugcggacau auagaaaucu ccccgcuaag auggucgacg aucaacugaa agccgaacuu     2460

caggccaccg cugagaagca ccaggugcug uccgacaagc ucuaccugua cuuuacgcag     2520

cugggacgcg aucuuuauac cggcgagaug cugaaaaugu cugaacucga gaacgacaug     2580

auugaucaca ucaugccuca aacauucgcc uacgacgaca gcgugaauaa ccggguucug     2640

gucucccaga aucauagcuc ucagcucggg aagaccuccg gcgaaaagag cgaugcgaag     2700

augauuagau ucuggcaagu gcugcgcgac aacaaacuua ucucugagca gaaguaucag     2760

aaucugacac ucgauuccga ccaccugagc aaaucuaccc gggaacagcu gcuuaacaga     2820

uaccuggugg agaagaaucc caucacgcag ugggucgcua gcgugcucac agagaaguau     2880

ggagauaacg gcacccgcgu ucugacaauu aacccgcgga ugguguacca aaugagacgc     2940

caguuuaauc ucgucaaccu gccugaagug aaugacuaug aucaugggcu ugacgccuac     3000

cuggccgcuu ucaucggaaa cuacguugau cggagauauc cccggcuccg cuccuacuuu     3060

guguauggcg acuucucuag cuccaaagag auugucuuua gacugaaucg guucaacuuu     3120

cuguucgauc uugggcaggc cgaacccaau gagcugaccg acugggaaac cggagagaug     3180

aucugggaca aggcggcugc caacagcgug cuccgcgaug uuuaccagua uaaauuuaug     3240

cugauuuccc aagccgugua cucuagacag ggcgcuaugu ucaaucagac aaucuauccu     3300

gccagccaag cugccgggcg gcagcucauc ccggucaaga aaaacagagc gacgaagcug     3360

uacggcggau acuccggcaa uguggacgcc uaucuugcua uugugcgccu cgggggaccc     3420

aagucugauc aguaccgggu cguuggcauc ccuaccagaa ugguggggcg ccugcagagc     3480

gccaugaaac aaggagcuga cacauaugcc aacacccucc acaagauucu gcagccccag     3540

uuuacaaaac ggaagaaaaa uagacagacc ggcuacuaug aggaaacgac agagccuuuc     3600

gccgugaucc uugauaaggu cuacuaucgc caacugauua ucgacgggca gcagaaguuc     3660

augcucggcu ccucuaccua ccaguuuaac gcuaaacaac uggugcucag cucccgggcg     3720

agacagauuc ugagcggaga ggaacgccuu accgaugaga auggcucuca ggaccugauc     3780

gauguuuacg acgaaauccu gacaaagauu gauagauauu ucgagcucua cgaccaacgg     3840

cacuuucgcc agucccugca ugaugggaga aaacaguuca ucagccuucc cauucacaac     3900

guguaucggg ccaaccgccu gaucgaacau ggaaaguacg agacccucuc ucagauucug     3960

aauggccucc acgcuaacac aaugaugacg aaucugagau aucuugggau cuccagcccu     4020

uuuggaaaau uccaaucucc caacggcauc aagcugucca aggacgccgu ccugguguac     4080

cagagcccgu ccgggcucuu ugaacggaga guucgccugc gggaccuuug a              4131


<210>  4343
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4343
augaacaaua agcccuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucaucacag augacuauaa aguuccuucu aagaaaauga aggugcucgg caauacggau      120

aaacacuuca uuaagaagaa ccugcuuggg gcucugcucu uugacgaggg aaccacagcc      180

gaagauagac ggcugaaacg caccgcgaga cggcgcuaca caagacggaa gaaucgccuu      240

agauaucugc aggagaucuu caccgaagag augagcaaag uggacauuuc cuuuuuccau      300

cggcucgaug acucuuuucu ggucccggaa gauaagcgcg gcagcaaaua ccccaucuuc      360

gcuacacugg aggaggaaaa ggaguaucac aagaacuuuc cuaccaucua ccaucucaga      420

aaacaccugg ccgacuccaa ggaaaaagcc gauuuccggc uuauuuaucu ggcucucgcc      480

cauaucauua aguaccgcgg gcacuuucug uacgaggagu cuuucgacau caaaaauaac      540

gauauucaaa agaucuuuaa ugaguucauu agcaucuaug acaacacguu ugaaggaucc      600

agccuuaaug gccagaacgc ucagguggaa gccaucuuca ccgacaaaau uucuaagucc      660

gcgaagagag agcggguucu gaaacucuuc cccgaugaaa agagcacagg ccuguuuucu      720

gaguuccuga aacucaucgu ggggaaucag gccgacuuua agaaacacuu cgaucuggag      780

gaaaaggcuc cucuucaauu uuccaaggac accuacgaug aggaccugga aaaccuccug      840

guccagauug gagaugacuu cgccgaucuu uuucuggugg cuaaaaagcu cuaugacgcc      900

auccugcuga gcggcauucu cacaguuacc gaucccucua cgaaagcgcc ucuguccgcc      960

agcaugaucg accgcuacga gaaucaucag aaggaccuug cugcccugaa acaguucauc     1020

aagacaaacc ucccggaaaa guaugaugag guguuuuccg accaaucuaa agauggguac     1080

gcuggauaua uugacggcaa gaccacacag gaggccuucu acaaauacau caagaaccug     1140

cuuagcaaac uggaaggggc cgauuauuuu cucgacaaga uugagagaga agauuuccug     1200

cggaagcaga gaaccuuuga caauggaucc aucccccacc agauucaucu gcaagagaug     1260

aacgcuauca uccgccggca gggcgaacac uacccuuucc uccaggagaa uaaagagaag     1320

auugagaaaa uccugaccuu ccgcauuccc uauuacgucg gcccucuugc gagagggaac     1380

cgggauuuug ccuggcugac aagaaauagc gaccaagcua uccgccccug gaacuucgaa     1440

gaaauugugg auaaggcccg gucugccgag gacuuuauca auaaaaugac gaacuaugau     1500

cucuaccugc cggaggaaaa ggugcugccu aaacacuccc uucucuauga gaccuucgcu     1560

gucuacaaug aacugacaaa gguuaaguuu auugccgagg gacugagaga cuaccaguuc     1620

cucgacagcg gccagaaaca gcaaaucgug acccagcugu uuaaggaaaa acgcaaggug     1680

acagagaaag auaucauuca guaucuucau aacgucgacu cuuacgaugg gaucgagcug     1740

aagggaauug aaaagcaguu caaugcgagc cucuccaccu aucacgaccu gcugaaaauc     1800

auuaaggaua aagaguuuau ggacgauucu aagaacgaag cuauccuuga gaauaucgug     1860

cauacgcuca caauuuucga agaccgggag augaucaaac aaagacuggc ccacuacgcc     1920

agcauuuuug augagaaggu uaucaaggcu cugacccgcc ggcauuauac cggcuggggg     1980

aaacucuccg ccaagcugau uaacggaauc uacgacaaac agagcaagaa aacaauccuu     2040

gauuaucuga uugacgaugg cgaaaucaau agaaacuuca ugcagcucau uaacgacgac     2100

ggccugucuu uuaaggagau cauucagaag gcucaagugg ucgggaaaac caaugaugug     2160

aagcagguug ugcaggaacu gcccggaucc ccugccauca aaaagggcau ccuucagagc     2220

auuaaacucg ucgacgagcu ggugaagguc auggggcacg cgcccgaauc uaucgugauu     2280

gagauggccc gggagaacca aacaacggcu cgcggaaaga aaaauuccca gcagagauac     2340

aagcggaucg aagaugcccu gaaaaaccuc gcucacggcc uggacagcaa uauucuuaag     2400

gagcauccca ccgauaacau ccaacugcag aaugacagac ucuuccugua cuaucugcag     2460

aacgggaaag auauguacac aggcaagucc cuugacauua aucagcucuc uagcugcgau     2520

aucgaccaca ucauuccuca agccuucauc aaagaugacu cccuggacaa ccgcguucug     2580

accucuagca aggauaaucg gggaaagucc gacaacgugc cgagccucga aauuguccag     2640

aaaagaaagg cguuuuggca gcagcugcuu gauucuaaac ugaucuccga gcgcaaguuc     2700

aauaaccuca caaaagccga gagaggcggg cuggacgaac gggauaaggu gggauuuauu     2760

aagcgccaac ugguugagac cagacagauc acgaaacaug uggcucagau ccuugacgcc     2820

cgguucaaua ccgaagucac agagaaggau aaaaaggacc gcagcgugaa aauuaucacc     2880

cugaagucua accucguguc caacuuuaga aaggaguucc ggcuguauaa aguucgcgaa     2940

auuaaugauu accaccacgc ucaugacccc uaucucaacg ccgucguggc caaggcuauc     3000

cugaaaaagu acccuaaacu ugagcccgaa uuuguguaug gcgauuacca gaaguacgac     3060

cugaagagau auauuagccg gacaaaagau ccuaaggagg ucgaaaaagc gaccgagaag     3120

uacuucuuuu auucuaaucu ccugaacuuc uuuaaagaag aggugcacua cgccgacggg     3180

acgaucguua agcgcgagaa uaucgaauau agcaaagaca caggagagau ugcuuggaac     3240

aaggaaaagg auuucgccac caucaaaaag gugcuguccc uuccccaagu caauauugug     3300

aaaaagacag aggaacagac cguuggccag aacgggggcc uguucgacaa uaacaucgug     3360

agcaaaaaga aggucgugga ugccucuaaa cucaccccga uuaaguccgg acugagcccu     3420

gagaaauacg gcggguaugc uagacccaca aucgccuacu ccguucucgu gauugcggac     3480

aucgagaagg gaaaagcuaa gaagcugaaa cggaucaagg aaauggucgg cauuacggug     3540

caagauaaaa agaaauuuga ggccaauccu aucgccuacc uugaagagug uggguauaag     3600

aacauuaauc ccaaccugau cauuaagcuc ccuaaauacu cucuguucga guuuaauaac     3660

ggacagagac gccugcuugc uagcuccauc gaacugcaga agggcaacga gcucaucgug     3720

cccuaucauu ucaccgcccu gcucuaccac gcgcagcgga uuaauaaaau cucugaaccg     3780

auucauaagc aauaugucga gacacaccag agcgaauuua aagagcugcu uaccgcuauc     3840

auuucccugu cuaagaaaua cauccagaag ccuaacguug agagccugcu ccagcaagcc     3900

uucgaccagu ccgauaagga caucuaucag cugagcgaau cuuuuauuuc ccuucugaaa     3960

cucaucagcu ucggggcccc cggcacauuu aaguuccugg gaguggagau uucucagucc     4020

aaugugagau accaaagcgu cucuuccugc uuuaacgcua cccucaucca ccagagcauu     4080

acgggccugu acgaaacacg caucgaucuu uccaaacugg gggaggacug a              4131


<210>  4344
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4344
auggacaaga aauacggcau cgggcuggau auuggaacca gcuccaucgg cuggacagug       60

gucgacaagu augggcacau uaucagaguu aaaggacaga cgggcauugg ggugcgccuc      120

uuccgggagg gacaaaccgc cgcugaaaga cggacauuuc gcaccacaag acggcgccug      180

ucuagacggc gcuggagacu ucggcugcuc cgcgagaucu ucgaugaacc caucagcgcc      240

auugacgaga acuuuuucgc gagacagaag gcuuccucuc uggccccugg cgaugcccgg      300

uaccagcagg cuuuucaacu uuucaaugac cgcaccgauc aggccuuuua uugcaaauac      360

ccgacaaucu aucaucugag acaggcucuc augaccgaaa gccggcaguu cgacauuaga      420

gagaucuacc uggcccugca ccaaauuauc aaguaucgcg gccauuuucu ccuuggggga      480

gcggccaagg auuuccaguc cgacaaacuu gaucuggagg cuuacuuuac gaccauuaac      540

gcccagcucg cuggcaucua cucugacggg agcgugcagc ugcccacacu ugauuggaau      600

caagccuguc aggaacugac cgacacaucc cggacccaga gcgacagaaa gaacuggcuc      660

gucaaagagc ugaagucuua uggagauucc caacugaaac cuaucuacac gcagauugcg      720

aaugccaucc ucggcuucaa gacagaccug gcuaagauua uggggaucaa cgaucccgaa      780

aaagcccuua gcuuuaaucu ggacgcucuc gaugaccacc gcgaugacgu ggccgagcug      840

cuuaccgaug agcagauucc ucugguuaac cuccuggcca agcugcauac agcucucgaa      900

cuggcggcca uccuucccga cggacagacc cugucuggca aaauggugga gaucuaugaa      960

acccaccgga agcaacucaa aacauacaag gcuuucauua gacagcugcc ugaucgccag     1020

cagcggcuug cccugaaaca agccuaugac cacuacaucg accagggccu caauagagag     1080

gcuuucuauu ccgaagucaa ggccauucug gagaagcaga aaccgcugac ggcgaccggg     1140

caggcuauga agaaagccau ggaucaaaac gccuuucucc ugaagcagcg cacaaaaacc     1200

aauaucgcua uucccuacca ggugcagcaa caggagaugg accagaucau cgccaaccaa     1260

agcaaguacu auccuuggcu ugcuacaccc aauccuguua agucucggag agugauugcc     1320

cccuacaaac uggaugaacu cgucugcuuc cgcgugaacu auuacguggg accgcugguc     1380

caguccaagc cuggcgaggc gaccaaagaa gccaaguuug cuuggaugac gcggaaagac     1440

ccccagagca caguugccau caccccuugg aauuucgauc agcaagugga cagacaggcu     1500

uccgccaacg cguuuauuca gcggaugaag accacagaua ccuaucugcu uggggaggac     1560

gugaucccca agaacucucu ccuguaccag aaauaugaag uccugaauga gcucaacaau     1620

gugcgcguua acggagauug gcuggacccc caaagaaagc aguaccuuuu caaaaagcug     1680

uuuauggauc agccucagag cguggucaca gugaaagccc uccaaaagac gcugguugug     1740

auuggcgcuu guccgacccu gcccacaguc gaggggcuug ccaauccuaa gcgguuccuc     1800

ucccagcuga ccacauacca ugacuuuugc aaacugcucc ccgaugacau ccagagagcu     1860

gaccgccaag ccgauauuga aaagaucauc aacuggagca ccauuuucga ggacacggcc     1920

aucuuuaaag aaaagcugcg ggauauuaaa uggcuuucug cugaccagau cagacgccug     1980

uccagcagac cucgguaucg cggauggggc agauucucug cgaagcuccu guguggccug     2040

cacgaucggc aggggaauac cgugauggac gagcuuuggc agacaagccg caacuuuaug     2100

caaauuauca gacaguccga uuuccagacc gccaucgcug ccgucaauca gcaagaccuc     2160

ggacggcagg aucugcagcg cgugauugac gaucuguaca caucucccca gaacaaaaag     2220

gccaucagac aaguucucgc uguggucacc gacauucaga aggccaugca uggcaaagcg     2280

ccgagcuggc uguucgugga aguugcuaag ggggcccaga aaaauccuca gcggacgcaa     2340

ucccgccaga gcaagcuggc cgaggcuuau agagagucug cccgggaacu gguggaugag     2400

gcggucaaag aacagcuccu gacacugccc gacgacagac aaaccuuuuc cgaucgccuu     2460

gugcucuacu uccagcagaa cggacgggac aucuauacag gcgagccucu ggauuuugag     2520

agacugagca auuaccacau ugaccacauc aucccccagg cugugguuaa ggaugacucu     2580

cucgauaacc gcguccugac cuccaaucaa gccaaccuug aaaagggggc ccaugugccu     2640

accauuuucc gggacaaaca ggagcccgug uggcaggaac uguauagagu cggccucauc     2700

agccaccgca agcugcggaa ccugcuuaca acgccggaug accuggauga cagaggacgc     2760

gcuaccggcu uuauugcccg gcagcucgug gagacaagac aaguuaucaa acuggugaug     2820

gaaguccucu cccaguacua cgacaccaca gagaccgagc ugauuucuau caaggcuggg     2880

cuuagccauc aguuccggca ggaacugaau cucccuaaac ugcgcgcccu gaacgauuau     2940

caccaugcgc aagacgccua ccuugcugcc agaaucggaa cguaucugcu ccagcgguac     3000

cccaaucuga agcgcuuuuu cguguauggc gauuuuaaga aaacacacuu cgaccuccag     3060

agacgguuua acuucauugg gggaaugauc uccagagaga agcaacccua caaucugauu     3120

aaaaacccug uuacccacca ggugaucugg gaugaagaga uggaaauugg cgacugccag     3180

aagaaaaauc cggagcagcc caagcaugau gcuaccguca agaugaugaa ccuuacaaaa     3240

aucaugaauc ugaagcacau ucucgugacc caugagguuu augaaaacca cggggcccug     3300

uuuaaucaaa caaucuaccc ugccucucag gcucacaaaa agaaacugau cccccagaag     3360

acggagaaac cuauugaacu uuacggcgga cauagcuccg agcaggcggg cuauauggcc     3420

aucauucgcc ugcucgggaa gcggcccgga uacaagguga ugagaguccc ugugcgcuau     3480

gugagccaac ugcagcacca ggacgucgcu gaacagaaaa agcuccugua caaaaucuuc     3540

guuucugagc uuaccucccg gaacaaggug aaacccuucg agguggucgu gccgcauguu     3600

ugucugaauc aaccugugga agauaugauu aagggccagu ggcacagauu ugcccugcgc     3660

acaaacgggu auuaccagaa ucagcaacag cucauccuga gccuugucgc ccagcggacc     3720

cuggcuggau auaagcccaa agacgaugag cucauggccg uguaccauga aaucugcaag     3780

caaguuaaau acuauuuccc ucuguacaag augaacaaau uucagucuag acucuucgcg     3840

gcugaggagc ugcuucagaa gcgcuccaca gugaacagcu uuucuauuac caucggcaag     3900

aaugggaaag aaaacaagac gcugaaucug ggcaaaagag agcuccugcu ucggcugcuc     3960

ggaggccugc acgccaacgc cacccgcguc gaccucaagg auauugggug uacaggacag     4020

uucggccaac uucaggugaa aaaugggauc aagcugaccg aacaggcugu ucugauucac     4080

gagagcccca caggacuguu ucauagacgg gugcgccuca acgaccugug a              4131


<210>  4345
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4345
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucaucacgg augacuauaa gguucccgcu aaaaagauga aagugcucgg caacaccgau      120

aagaaguaca uuaaaaagaa ucugcuuggg gcccuucucu ucgacucugg agagacagcg      180

gaagcuacca gacugaaacg gacagcccgc agacgguaua cccgcagaaa gaaccggcuu      240

cgcuaccugc aggagaucuu ugccgaagag augacaaaag uggaugagag cuucuuuuau      300

agacucgacg aguccuuccu gaccacggau gagaaggacu uugaacggca cccuauuuuc      360

ggcaauaagg cugaugagau caaauaccau caagaauuuc cgaccaucua ucaucugcgc      420

aagcaccucg ccgacucuag cgagaaagcu gaucugagac uugucuaccu ggcccucgcg      480

cacaugauua aguuccgggg gcauuuucug aucgagggag aacuuaacgc cgagaauaca      540

gacgugcaga aaauuuucgc ugauuuuguu ggcguguacg accgcaccuu cgacgauucc      600

caccugucug aaaucacagu cgacgccgcu agcauucuca ccgagaagau cuccaaaagc      660

agacggcugg aaaaccugau uaaguauuac cccacggaga agaaaaauac acucuuuggc      720

aaccugaucg cccuugcgcu ggggcuccag ccuaauuuca agaugaacuu caaacugucu      780

gaggaugcca agcugcaguu uuccaaagac agcuauaaug aagaucugga ggaacuccug      840

ggaaagaucg gcgacgauua cgcugaccug uucaccucug ccaagaaccu cuaugaugcu      900

auucugcuuu ccgggauccu gacaguggac gauaauagca ccaaagcgcc ccucucugcc      960

uccaugauua agcgcuacgc ugagcaccau gaagaccugg agaaacuuaa ggaguuuauc     1020

aaagcgaaca agagcgaacu guaucacgac auuuucaagg augagaccaa aaacggauac     1080

gccggcuaca ucgaaaaugg gguuaagcaa gacgaguuuu auaaauaccu caagaacaca     1140

cuguccaaaa ucgcuggauc ugauuauuuc cuggacaaga uugaaagaga ggauuuucuc     1200

cggaagcaga gaacguucga caauggcagc aucccucauc agauucaucu gcaggagaug     1260

cacgccaucc uucgccggca aggcgauuac uaucccuuuc ugaaagaaaa ccaggacaga     1320

auugagaaga uccucaccuu ccgcaucccu uacuaugugg ggccgcuggc ccggaaagau     1380

uccagauuua gcugggcuga auaccacucu gacgagaaga uuacacccug gaauuucgau     1440

aaagucaucg acaaggagaa auccgccgaa aaguuuauua cccgcaugac acugaacgau     1500

cuuuaccucc cugaggaaaa ggugcugccc aaacacagcc auguguauga gaccuacgcg     1560

gucuauaaug aacugacgaa gaucaaauac guuaacgagc agggaaagga cucuuucuuc     1620

gacagcaaua ugaaacagga gauuuuugau cacguguuca aggaaaaccg gaaggugaca     1680

aaagagaagc uccugaauua ucuuaacaaa gaauuuccug aguacagaau caaggaccug     1740

auuggccucg auaaagaaaa uaaguccuuc aacgcuucuc uggggaccua ucaugaccug     1800

aagaaaaucc uugauaaggc cuuucucgac gauaaaguca augaggaggu gaucgaagac     1860

auuaucaaga cccugacacu guucgaggau aaagacauga uucacgaacg ccuccaaaag     1920

uacagcgaua ucuuuaccgc cgaccagcug aagaaacuug agcggagaca cuacacagga     1980

uggggccgcc uguccuauaa gcucauuaac gggauccgga acaaagaaaa uaacaagacg     2040

auccuggauu accugauuga cgacggaagc gcuaauagaa acuucaugca gcuuaucaau     2100

gaugacaccc uccccuuuaa acaaauuauc cagaagucuc agguuguggg cgaugucgac     2160

gauauugagg ccgugguuca ugaccugccg ggcuccccug cuaucaaaaa ggggauccug     2220

cagagcguga agauugucga ugagcucgug aaagucaugg gagacaaccc cgauaauauc     2280

gugauugaaa uggcccggga gaaccaaaca accaaucgcg gcagaucuca gucccagcag     2340

cggcugaaga aacuucaaaa cagccugaag gaacucgggu ccaauauccu gaacgaggag     2400

aaaccuucuu auauugaaga caagguugag aauagccauc ugcagaacga ucagcuuuuc     2460

cucuacuaua uccagaaugg aaaggacaug uacacaggcg acgaacugga uaucgaccac     2520

cuguccgauu augacauuga ucacaucauu ccccaagcgu uuaucaaaga cgauucuauu     2580

gacaacagag ugcucaccag cuccgccaag aaccgcggga aaagcgauga cguccccucu     2640

cuggauaucg ugcgggcuag aaaggccgag uggguucgcc uuuacaaauc cggccugauu     2700

agcaagagaa aguucgacaa ucucacgaaa gcugaacggg gaggccugac cgaggccgau     2760

aaggcggggu uuaucaaacg ccagcuggug gagacaagac agaucaccaa gcacgucgcc     2820

cagauucuug acgcucgguu caacacagaa ucugacgaga augauaaagu gauccgcgac     2880

gugaagguua uuacccugaa guccaaccuc gucagccaau ucagaaaaga uuuugaguuc     2940

uacaaggugc gggaaaucaa ugacuaucau cacgcccaug augcuuaccu gaacgccgug     3000

gucggaacgg cccuccugaa aaaguauccu aaacuugcuu cugaauuugu guacggcgag     3060

uauaagaagu acgacguuca caaacugauu gccaagagcu ccgaugacca uagcgagaug     3120

gggaaagcca cagcuaagua uuucuuuuac ucuaaucuca ugaacuucuu uaaacgcgug     3180

aucagauacu ccaauggaaa ggucaucgug cggccgguug uggaauauag caaagauacc     3240

gaggacauug ccugggauaa gaaguccaac uuccgcacaa ucugcaaagu ccugucuuac     3300

ccccagguga auauuguuaa gaaaguggaa acccagaccg gcggguuuag caaggagucc     3360

auccugccua aaggcgacuc ugauaaacuu auucccagaa agacaaagaa ggccuauugg     3420

gacacgaaaa aguacggagg cuucgacagc ccuaccgucg cuuauuccgu guuugugguc     3480

gccgauguug aaaaagggaa ggcgaagaaa cugaagacag ugaaagagcu cgugggaauc     3540

ucuaucaugg agcggagcuu cuucgaagag aaccccgucg aauuucugga gaauaagggc     3600

uaccacaaca uuagagagga caaacucauc aagcugccga aguauucccu uuucgaauuu     3660

gaagggggaa aacgccggcu gcucgcuagc gccucugaac ugcaaaaggg caaugagaug     3720

gugauuccug ggcaccuggu uaaacuucug uaccaugccc agagaaucaa cuccuucaac     3780

agcaccaagu accucgauua ugugucugcu cacaaaaagg aauuugagaa aguccugucc     3840

uguguggagg acuucgccaa ucucuacguu gauguggaaa agaaccugag caagauucgc     3900

gcggucgcug acucuaugga uaauuuuucc aucgaggaaa uuagcaacuc cuucaucaau     3960

cuucugacac ugaccgcccu cggcgccccc gcugacuuua acuuccuggg agagaaaauc     4020

ccucggaaga gauauacgag cacaaaagaa ugccuuaaug ccacccugau ucaucagucu     4080

aucaccggcc ucuacgagac acgcauugau cugagcaaga ucggggagga auga           4134


<210>  4346
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4346
augaagaaac ccuacagcau cggccuggac auugggacca acuccguggg augggccguc       60

aucacagaug acuauaaggu uccuucuaaa aagaugaaag ugcucggcaa uacggauaag      120

cacuucauua agaaaaaccu gaucggggcc cuucuguuug acgagggaac cacagccgaa      180

gauagacggc ucaagcgcac cagcagacgg cgcuacacaa gacggaaaaa ucgccugaga      240

uaucuucagg agauuuucuc cgaagagauc ucuaagcugg acagcuccuu uuuccaucgg      300

cucgaugacu cuuuucuggu gccggaagau aaacgcggca gcaaguaccc caucuucgcg      360

acccuggagg aggaaaagga guaucacaaa aaguuuccua caauuuacca ucucagaaaa      420

caccuggcug acuccaagga gaaaaccgau cuucggcuga ucuaucucgc ccuggcccau      480

augauuaagu accgcgggca cuuccuuuac gaagagagcu uugacaucaa aaacaaugau      540

auucaaaaga ucuucaacga auuuauuucu aucuaugaca auacguucga gggauccagc      600

cugucuggcc agaacgcuca ggucgaagcc aucuuuaccg acaagauuuc caaaagcaca      660

aagagagagc gggugcucaa acuguucucu gaugaaaagu ccaccggccu guucagcgag      720

uuucucaaac ugaucguugg gaaucaggcu gacuucaaga agcacuuuga ucuugaggaa      780

aaagcucccc ugcaauucuc caaggacaca uacgaugagg accucgaaaa ccugcuugga      840

cagauuggcg auggguuugc ggaccuguuc cucguggcca aaaagcugua ugaugcuauc      900

cugcucucug ggauucugac cgucacggac ccuagcacaa aagcuccccu uuccgcuagc      960

augaucgagc gcuacgaaaa ucaucaggag gaucuggccg cgcucaagca guuuaucaag     1020

aacaaucugu cugagaaaua ugccgaagug uucuccgacc aaagcaagga cggcuacgcu     1080

ggguuuauug auggaaaaac cacacaggag gccuucuaua aguacaucaa aaaccuucug     1140

ucuaagcucg aaggcgcuga cuacuuucug aauaagauug agagagaaga uuuccugcgg     1200

aaacagagaa ccuuugacaa cggcagcauc ccucaccaga uucaucucca agagaugaac     1260

gccauccugc gccggcaggg ggagcacuau cuuuuccuga aggaaaauag agagaaaauc     1320

gaaaagauuc ucgccuuccg caucccguac uauguuggac cccuggcucg gggcaacaga     1380

gauuuugcgu ggcugacccg caauuccgac caggccauuc ggccuuggaa cuucgaggag     1440

guggucgaua aagccucuag cgccgaagac uuuaucaaua agaugacaaa cuacgaucuu     1500

uaucuccccg aggaaaaagu gcugccuaag cacucccugc ucuacgagac guucgccgug     1560

uauaaugaac ugaccaaggu caaauuuauu gcugaggggc uuagagacua ccaauuccug     1620

gauagcggac agaagaaaca gaucguuaac cagcucuuua aggacaaacg caaggugaca     1680

gagaaggaua uuauccauua ccugcacaau guggacggcu augacgggau cgaacugaaa     1740

ggaauugaga agcaauucaa cgccucucuu uccaccuacc augaucuccu gaaaaucauu     1800

aaggacaaag aauuuaugga uaaucccaag aacggcgaga uccuggaaaa uauuauccac     1860

acacucacca ucuucgagga ccgggagaug auuaagcaga gacuggcgca guaugauacg     1920

cuuuuugacg aaaaagucau caaggcucug acacgccggc auuacaccgg cugggggaaa     1980

cucagcgcca agcugauuaa cggaaucaga gauaaacaga ccggcaagac aauucuggag     2040

uaucuuaucg acgaugggga cugcaaucgg aacuucaugc aacucaucaa cgaugacgga     2100

cugucuuuua aggaaauuau ccagaaagcc caggugguug gcaagaccga ugacgugaaa     2160

caggucgugc aagagauucc gggguccccu gcuaucaaga aaggcauucu gcagagcauc     2220

aagaucguug acgaacucgu gaaagucaug ggacacaauc ccgaguccau ugugaucgag     2280

auggcccgcg aaaaccagac aacggcuaag ggcaagaaaa auucucagca aagauacaag     2340

cggauugagg augcccugaa aaaccuugcg ccugggcugg acagcaauau ccucaaggaa     2400

aaccccaccg auaauauuca gcugcagaac gacagacugu uccuuuauua ccuccaaaau     2460

ggaaaagaua uguacacagg caaggccauc gacauuaacc agcuguccaa uuaugauauc     2520

gaccacauca uuccccaggc uuucaucaag gaugacucuc uggauaaccg cguccucacc     2580

agcuccaaag acaaucgggg aaagagcgac aacgugccuu cuauugaggu ugugcagaaa     2640

agaaaggccu uuuggcaaca gcugcuugau uccaaacuga ucagcgagcg caaguucaau     2700

aaccucacaa aggcuaaaag aggaggccug gacgaacggg auaaggucgg guuuauuaaa     2760

cgccagcugg uggagaccag acagaucacg aagcauguug cccaacuucu ggacacccgg     2820

uucaacacag aagugaauga ggaaaaccag aaaauccgca ccgucaagau uaucacacuc     2880

aagucuaauc ugguguccaa cuuuagaaaa gaguucggcc ucuacaaggu gcgggagauu     2940

aaugauuauc accaugcgca cgacgccuac cugaacgcug uugucgccaa agcuauccuu     3000

aagaaauauc cgaagcugga acccgaguuu guguacggag auuaucagaa guacgaccuc     3060

aaacgcuaca uucuuaagag caaagccucu aauaccaucg auaaggccac ggaaaaauau     3120

uucuuuuaca gcaaccugcu gaauuucuuu aaggagaaag ugagauaugc ugacggcaca     3180

aucaagaagc gggaaaacau ugaguacucc aaugauaccg gggagaucgc guggaacaaa     3240

gaaaaggacu ucgccacaau uaaaaagguc cugagccucc cucaggugaa uaucguuaaa     3300

aagaccgagg aacaaaccgu gggacagaac ggcgggcugu ucgauaauaa cauugucucu     3360

aagaaaaagg ugguugacgc uuccaaacuc auccccauua agagcucccu gucuccugag     3420

aaauauggag gcuacgcccg ccccacaauc gccuauagcg ugcuugucau cgcugacauu     3480

gaaaagggga agggcaaagc caagaaacug aagagaauca aagagauugu gggaaucacg     3540

auucaggaua agaagaaauu ugaguccaac ccgguuaccu accucgaaga guguggcuac     3600

aagaauauca acucuaaucu gaucauuaaa cugccuaagu auagccuuuu cgaauuuaac     3660

gacgggcaac ggagacugcu cgcguccucu aucgagcugc agaaaggaaa ugagcucauu     3720

cugcccuacc accuuacagc ucugcuguau caugcccagc gcaucaacaa gauuagcgaa     3780

ccuauccaca aacaguacgu ggaggcccau caaaaugagu ucaaggaacu ccugaccaca     3840

aucauuuccc uuagcaagaa auauauccag aagcccaacg ucgaacugcu ccugcagcag     3900

gcuuuugauc aagccgacaa agauauuuac cagcucucug aguccuucau cagccugcuu     3960

aagcugaccu cuuuuggcgc gccuggggcu uucaaauuuc ugggagugga gauuucccag     4020

agcucugugc gguauaagcc caauucccag uuccucgaca cgacacugau ccaccaaagc     4080

auuaccggcc uuuacgaaac cagaaucgau cuguccaagc ucggggagga cuga           4134


<210>  4347
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4347
augaacaaua agcccuacag caucggccug gacauuggga ccaacuccgu gggauggucu       60

gucaucacag augacuauaa aguuccuagc aagaaaauga aggugcucgg caauacggau      120

aaaagauuca uuaagaagaa ccugaucggg gcccuucugu uugacgaggg aaccacagcu      180

gaagcccggc gccucaaaag aaccgcgcgg cgcagauaca cacggcgcaa gaauagacug      240

cgguaucuuc aggagauuuu cgcugaagag auguccaaag uggauucuag cuuuuuccac      300

cgccuggacg auuccuuucu caucccggaa gacaagagag gcucuaaaua ccccaucuuc      360

gccacccugg ucgaggagaa ggaauaucau aagcaauuuc cuacaauuua ccaucugcgg      420

aaacagcucg ccgauagcaa ggagaaagcu gaccugcgcc uuaucuaucu ggugcucgcc      480

cacaugauua aguacagagg gcacuuccug uacgaagagg cuuuugauau caaaaacaau      540

gacauucaga agaucuucaa cgaguuuauu uccaucuaug auaauaccuu cgaaggaagc      600

ucucuuuccg gccagaacgc ccaaguugag acgaucuuua ccgacaaaau uagcaagucu      660

gcgaagcggg aacgcgugcu gaaacucuuc cccgacgaga aguccacagg ccuguucagc      720

gaauuucuga aacucaucgu cgggaaucag gccgauuuca agaaacauuu ugaccuggag      780

gagaaggcuc cucuucaguu cucuaaggau accuacgacg aagaucugga gaaccuccug      840

gaacagauug gagacgauuu ugccgaccuu uucguggcug ccaaaaagcu guaugaugcg      900

auccuccugu ccggcauucu gacaguuacc gaccccagca cgaaagcccc ucucuccgcu      960

ucuaugaucg agagauacga aaaucaccaa aaggaucugg ccgugcuuaa acaguuuauc     1020

aagacaaacc ugccggagaa guaugucgag guguucagcg accaguccaa agacggguac     1080

gcuggauaua uugauggcaa gaccacacag gaagccuuuu acaaauacau caagaaucuc     1140

cugagcaaau ucgacggggc cgauuauuuu cuugacaaga uugagcggga aguguuccug     1200

agaaagcaac gcaccuuuga uaacggaucu aucccccacc agauucaucu ccaggagaug     1260

aacaccaucc ugcggagaca aggcgaacac uacccuuucc ugaaagagaa uaaggagaaa     1320

aucgaaaaga uucucacauu ccgcaucccc uaucaugucg gcccucuggc ucgggggaac     1380

agagacuuug cguggcuuac gcgcaauucc gaucaggcca uucggcccug gaacuucgag     1440

gaaaucguug acaaagcuag cucugccgag gauuuuauua auaagaugac caacuacgac     1500

cuguaucucc cggaggaaaa agugcugccu aagcacagcc ugcuuuacga gacauucgcc     1560

guguauaaug aacucaccaa ggucaaauuu aucgcugagg gaaugagaga uuaccaguuc     1620

cuggacuccg gccagaagaa aaagauuauc aaccaacugu uuaaaaagaa gcgcaaagug     1680

acagaagaug acaucauuga cugccuccag aagaucgaua auuacgacgg gauugagcug     1740

aaaggaaucg agaagcaguu caacgccucu cuuagcaccu aucacgauau ucugaaaauc     1800

auccgggaca aggaauuuau ggaugacccc aagaauggcg auauucucga gaacguuauc     1860

cauacgcuga caauuuucga agacagagag augaucaaac agcgccuggc gcaauacaau     1920

uccauuuuug augaaaaggu gaucaaagcu cuuagccgga agcacuauac cgggugggga     1980

aaacucucug ccaagcugau cgacggcauu agagauaagc agaccggcaa aacaauccug     2040

gacuaccuca uggaugacgg gaaguccaac cggaauuuca ugcagcugau uaaagacgau     2100

gagcuuagcu uuaccgagau cauucagaag gcccaagucg ugggagacac agauaaccug     2160

cgccagguug ugcagaaccu cccuggcucu cccgcuauca aaaaggggau ccugcagucc     2220

auuaaaaucg ucgacgaacu ggugaagguc augggacaug cccccgagag cauugugauc     2280

gaaauggcua gagagaauca aacgaccgcc cggggcaaga aaaacuccca gcagagauau     2340

aagcgcauug aagauucucu uaaaaaucuc gcgagcgggc uggacuccaa cauccugaag     2400

gagaauccua cagauaacau ucaacuccag aaugaccggc uguuccuuua cuaucugcag     2460

aacggcaaag auauguacac cggagaggcc cucaauauca accagcuguc uagcuacgac     2520

aucgaucaca uuaucccgca ggcuuucauu aaggacgacu cccuggauaa uagaguucuu     2580

acaagcucua aggacaaccg cggcaaaucc gauaaugugc ccagccucga agucguggag     2640

aagaugaaag ccuuuuggca acagcugcug gacucuaagc ucaucuccga aagaaaauau     2700

aacaaucuga ccaaggcuga gcgggagcgc gggggacuua acgaacugga uaagguuggc     2760

uucauuaaaa gacagcucgu ggagacgcgg caaaucacca agcaugucgc ccagauccug     2820

gacgcgcgcu uuaacaaaga agugacagag aagaauaaaa agaacagaaa ugugaagauu     2880

aucacccuga aaagcaacau gguuucuaau uuccggaagg aauuugggcu uuacaaaguc     2940

cgcgagauua acgauuauca ccacgcccau gacgcuuacc ugaaugccgu gguggcuaag     3000

gccauccuca aaaaguaucc uaagcuggag cccgaauuug ucuacggaga uuaucagaaa     3060

uacgaccuca agagauacau uagccggucc aaagauccua aggaggugga aaaagccaca     3120

gagaaguauu ucuucuacag caaccugcuu aacuuuuuca aagaagaggu ucacuaugcu     3180

gacggcacca ucgugaagcg cgagaauauc gaauacucua aggauacggg ggagauugcg     3240

uggaauaaag aaaaggacuu ugccacaauc aaaaaggucc ugucccuccc ccaggugaac     3300

auuguuaaaa agaccgagga acaaacagug ggccagaaug gaggccuguu cgacaacaau     3360

aucgucagca agaaaaaggu gguugaugcu uccaaacuga uuccgaucaa gucugggcuu     3420

agcccugaga aauauggagg cuacgccaga cccaccauug ccuauuccgu gcuggucacc     3480

uacaagcaga acaacaagau caaaaauaca auggugggga ucccugugcu cacgaagcug     3540

gaguucgaac gggaccccaa ccucuaccug agaaaucuug gaauuaacaa ugucucucau     3600

guuaucaaac uggugaagua uaccgugcuc gaguuuaaac acaaguacgg cacauaucgc     3660

cgguucauug ucagcaacca agaacugaag agagcuaauc agaucuuugu gaccacaaaa     3720

cagaugaagc ugauuuccca guacaccacg cuugauaaaa acgagcacga cucucugcuc     3780

aagaauaacc aacagcugau ccagagcauc uggaaucagu uccucgaguu uaugcuggcc     3840

acagaucuug uuaacaaaaa gcaauaugac gaacugauuc ugaccaauaa ccagaccauc     3900

gaggaauguc gcaauauuau gaaccgggug aucacaaccc ucuccuucac aaauauuaac     3960

gcggggcaga aaaagaucau cuuugaugac acgcugagcc uuccuauuaa gagauggcag     4020

uucaccgauu cugacaaaau ggucaagaaa aucauugagu ccacacugau ccaucaaagc     4080

auuaccggcc ucuacgaaac acgcaucgau cugucuaagc ucggagagga cuga           4134


<210>  4348
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4348
auggagagca accacgacgg cgaacaggug aaguacaccc uggggcucga uaucggaaca       60

uccucuauug gcuaugccgc uaucgacaaa caucaaaagc ccauuagagc caaagggaag      120

cacgucaucg gaguucggcu guuccaggag ggccagacgg cggcugaucg cagagccuuu      180

cggaccacac gcagacggcu uaaacgcaga aaguggcggc ugaaucuccu gaaccgccuu      240

uucgacccuu accugagcga aguggauccg aauuuucucc ccagacugcg gcaguccaac      300

cugucuccua aggacgauaa uaaacauuuc aacgggagca cccucuuucc cgacaagaca      360

gaugccgcuu ucuaucgcca auacccuacc auguaucacc ugagauacgc ccuuaugaca      420

gagaaacgga aguuugacau ucgcgaagug uaucuggcua uccaucacau cgucaaauac      480

agaggaaauu uccucaacuc cgccaccgug gauucuuuua agacgagcaa uauugacuuc      540

accucccagu uugaucggcu gaacgagcuu uacagacagg uuauccugga cgagcccuuc      600

cagauuaaua uggaccaagu ggaugaaaug acaaacaagc uccuggacaa ugaugcgcug      660

aaacucgaca cccagaagca ggucgccaaa cugcuuccug ugaucuauaa cgauaagccg      720

auugacaaac aguacacacg caucgcuacc gaguuuagca aggccauucu gggcuauaaa      780

acgaagcucg auguuauccu gaaucuugac acacaaaacg cuaaggauug ggugauccgg      840

cuggacgaug aagacauuga cgauaaacuc cccgcccugg ucgagaaccu ggacgagucu      900

agacaggaaa ucgugaccau uauccgcgau cucuacgcgc agauuacacu gaaugccauc      960

gugccuaagg gcaaaucccu uagcgagucu auggucgaca aguauaacga ucacaaagac     1020

caucuggauc uccugauggg gcuuauccac gaacugggag acgauuccgc uaagggcauu     1080

aagcuccggg aggccuacac ccaauauguu gggaaaagcg acgauaagac ccugaaucag     1140

gacgauuucu acgcugccau caaaaagaac cuggacgaag acucuaaaag aucccccaag     1200

auucagcgcc ucaucgagca ggccagcuuc augccuaagc aacggacauc cgcuaaugga     1260

gugauucccc aucagcugca ccagcuugag cuggauagaa ucaucgaaaa ccagggccgc     1320

uacuauccgu uucucaaaaa gccuaauccc aacacgcaca aaccuaaugg gggaaaguac     1380

aaacuggacg agcugguggc guuccggauu cccuauuacg ucggcccccu uaucaccaag     1440

agagaucaag aaaagacauc uggcgccgac uuugcuugga ugauucgcaa accugggcag     1500

gagggagaaa ucaccccgug gaacuucgau cagaaggugg accggauggc cagcgccaau     1560

agauuuauuc ggcgcaugac aaccaaagau uccuaucucg uugcugagga cgugcugccc     1620

gaugccagcc ugaucuacga gaaguucaaa gcgcucaacg aacugaauau gcuuaagguc     1680

aacgacucua gacugucccc uagccagaaa caagaucucu uuaauaaccu guucaagaag     1740

cagaaaacga uucggacaaa gaaacugcag accuauauca gaaccaacug ggagcuuccc     1800

ucugugauga ucagcggccu cgcugacccu gauaaguuua auuccucucu gagcacauac     1860

auugacuucc gcgacaucuu uggggaaaaa guugaugacc ccaaccggca ggccgauuac     1920

gaggaaauua ucgagugguc caccguguuc gaggacagaa agauuuauca agccaagcug     1980

aaucagcucg auuggcugac agaaccgcag cgcaaagccc uucugacgaa gagauacacc     2040

ggauggggcc ggcucagcaa aaagcugcug acagggcuua aagaccaaaa cggagauucu     2100

auccucgacc agcuguggaa gaccaaugau aacuuuaugc agauccaguc ccgcgacgag     2160

uucgcuaagc aaauucauga ugaaaaugcu aaacaguucc acgaggccaa caaugucgac     2220

gacauccugg augacgcguu uacaagcccu cagaacaaga aagccauuag acagguggau     2280

aaggucgugc aagacguugu gaaagcuguc ggcuaugccc ccgauaagau cgcuauugag     2340

uucacccggu cucucgaaaa uaacccucag cgcacggugu ccagacagcg gcagcugcaa     2400

gccgcguaca agaauauccc ccgcgagauc augaaaagcg gccuuauggc cgaacugggg     2460

uccguugcug acucuaagaa agugcucagc gauaagcugu aucuguacuu uacccagaug     2520

ggaaaagaca uguaugagga uaagacaauu aacaucgaca aucuuccuaa cuacgauauu     2580

gaccauaucc ucccccaggc cuucauuaaa gaugacucca ucgacaacag aguccugauc     2640

ucuaaggaag agaaugcucg caagagcaac cgggugccgc ugaaauuuuu cggcgccaag     2700

agaauggggu uuuggaaaga ucuccugaau aacggacuua uuuccaaucg gaaguuccaa     2760

aaccugacca cagacccuga gaaaaucagc aaguacucug ccaagggcuu uauucacaga     2820

cagcucgugg aaaccuccca gguuauccgc cuggucgcua auauucuggg gaacgaguau     2880

agccaggcgg gcacgacaau cauugaagug ucugccaaaa ugaaucggca acuuagagag     2940

gauuuccauc ugaucaagaa ccgcaaugug aacgacuacc accacgcuau ggaugccuau     3000

cucaccgccu acgucggaga cuaucuguac cuccgguauc ccaaacugag acccuacuuu     3060

guguacggcg auuucaagaa agcugaagac caggaucuua auauccgcaa cuuuaauuuc     3120

cugcaugacc ucaaggauaa cgagucccug gacaagauug uugauagcga gacaggggaa     3180

aucguguggg agaaacacgg aucugucaag cagcugaaaa agguguauca uuacaaauuc     3240

augcuuguua gccaggaagu guauacccgg caagacgccc uguuuaacca gaccauuuac     3300

ccugcguccg acgcugauaa gagaaagcuc aucccgauua aaaauagcaa gcccgucgac     3360

guguauggcg gguacucugg aaacaucgau gccuauaugg ccaucguucg cgugcacggc     3420

aaaaaggagg acaauuacaa agucgugggg auuccuaugc gggcugugac aaagcugaag     3480

aaagccgaaa aggagggcca cgauuccuac cucaacgcgg uccaugaggu ucugaaaccc     3540

caguucacga agaaaaagaa aaauagaaag accggagaaa ucacagagac cguggacgaa     3600

uuugaugugc uucugggcaa ggucuauuac cggcagcuca uuguggacgg gaacaaaaag     3660

uucaugcugg gaagcuccuc uuaucaauac aaugcuaaac agcugguucu uagcgauaag     3720

gccaugcaag ugcuguccaa aaacggcaag gucaagucuc aggacgagaa ucagaaccuc     3780

aucgaugugu augacgagau ucuggaaaaa guugaccagu acuuugagcu cuaugaucag     3840

cgccacuaca gagaaagccu gcauaagggg cggggaaucu ucgugaaucu uccucugucc     3900

aaccaauacg acggcucuaa acugacaagc uaugggaagu acgagaccau cgaugccauu     3960

cucaauggcc ugcacgcuaa cgccacgaug uccaaucuuc gcuaucuggg aaucagcucu     4020

cccuuuggca aaaugcaggc uuccaacggg cucacaauug acccugauag caucauuugc     4080

uaccagucuc ccaccggacu guucgaaaga cagaucaagc ucuccgaccu guga           4134


<210>  4349
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4349
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcc cuucuguucg acggaggcaa caccgcugcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acaaugcaag aggaaaagua cuaucaugag aaauucccca ccaucuacca ucugcggaag      420

gaacuggcug acaagaaaga gaaggccgau cuccgccugg ucuaccuugc ucuggcccac      480

auuaucaaau uuagaggaca cuuccucauu gaggacgauc gguuugacgu gcgcaacaca      540

gacauccaga agcaguauca ggcguuccug gaaauuuuug auaccacguu cgagaauaac      600

caucuucugu cccaaaaugu ugacguggaa gccauccuca ccgauaaaau cucuaagagc      660

gcuaaaaagg accggauucu ggcccaguac ccuaaccaga aguccacagg caucuuugcu      720

gaguuccuga aacucauugu cggcaaucag gccgauuuca agaaacacuu uaaccuggaa      780

gacaagacac cccuucaauu cgcgaaagau agcuaugacg aggaucugga gaaucuccug      840

gggcagaucg gagacgaauu ugccgaucuu uucucugugg cuaagaagcu guacgacucc      900

guucuccuga gcggcauucu gaccgugacc gaccucucua cgaaagcccc ucuguccgcu      960

agcaugaucc agagauauga ugagcaccau gaagaccuua agcaccugaa acaguuuguc     1020

aaggccucuc uccccgagaa cuacagagaa guguucgccg auuccagcaa agacggguau     1080

gcuggauaca ucgagggcaa gacaaaucaa gaggcguuuu acaaguaucu gcuuaaacug     1140

cucaccaagc aggaaggguc cgaguacuuc cuggagaaaa uuaagaacga agauuuucug     1200

cgcaaacagc ggacauucga caacggaucu aucccucauc aagugcaccu caccgaacug     1260

agagccauua uccgccggca gagcgaguau uacccguuuc uuaaggagaa ucaggauaga     1320

auugaaaaga uccugaccuu ccgcaucccc uauuacgucg gcccucucgc ucgggagaaa     1380

uccgacuuug ccuggaugac aagaaagacg gaugacagca uucgccccug gaacuucgaa     1440

gaucugguug acaaagagaa gucugccgag gcuuucaucc accggaugac caauaacgau     1500

cuguaucuuc cugaagagaa agugcucccc aagcauuccc ugauuuacga aaaauuuaca     1560

guguacaaug agcugaccaa ggucagauuc cucgccgaag gcuuuaagga cuuccaguuu     1620

cugaaccgca aacaaaagga gacaaucuuc aauagccuuu uuaaagagaa gcggaaagug     1680

accgaaaagg acauuaucuc uuuccugaac aagguugaug gguaugaggg aauugcgauc     1740

aaaggcaucg aaaagcaguu uaaugcuagc cucuccacgu accacgaccu gaaaaagauu     1800

cuggggaaag auuuccuuga caacacagau aaugagcuca uccuggaaga cauugugcag     1860

acccugaccc ucuuugagga uagagagaug aucaagaagu gccuggacau uuauaaagau     1920

uucuuuacag aaucucagcu uaagaaacug uaccggcgcc auuauaccgg auggggcaga     1980

cucagcgcca agcugaucaa cgggauccgg aauaaagaga accaaaagac aauucuggac     2040

uaccuuaucg augacggauc cgccaauaga aacuucaugc agcucauuaa cgaugacgac     2100

cugagcuuca agccgaucau ugauaaagcu cgcacgggcu cucacuccga caaucugaag     2160

gaagucgugg gcgagcucgc cgggagcccu gcuaucaaaa agggaauccu gcagucucuu     2220

aaaauuguug augaacuggu gaaggucaug ggcuaugagc ccgagcagau cguggucgaa     2280

auggcccggg agaaccaaac cacagcgaaa gggcucucca gaagccgcca gagacugacc     2340

acacugcggg aaucccuugc caaucucaag ucuaacauuc uggaggagaa gaaaccuaag     2400

uacgugaaag accagguuga aaaucaucac cugagcgaug accgccucuu ucuguacuau     2460

cuucagaacg gaagagauau guacaccaag aaagcucugg acaucgauaa ucucucccaa     2520

uaugacauug aucacaucau cccccaggcc uucauuaagg acgacucuau cgauaaccgg     2580

gugcugguca gcuccgcuaa gaaucgcggc aaaagcgacg augugcccuc uauugagauc     2640

guuaaggcca gaaaaauguu uuggaagaac cugcuugacg cgaaacugau gucccagcgg     2700

aaguacgaua aucucacgaa ggccgaacgc gggggccuga ccagcgacga uaaagcuaga     2760

uucauucaac ggcagcucgu ggagacacgc cagaucacca agcaugucgc ccggauucug     2820

gacgaaagau uuaacaauga gguggauaac ggcaaaaaga ucuguaaagu gaagaucguu     2880

acacuuaagu cuaaucuggu cuccaacuuc agaaaagagu uuggauucua uaagauucgc     2940

gaagugaacg acuaccacca ugcucacgau gccuaucuca augccguggu cgcuaaagcg     3000

auccugacca aguacccuca gcuggagccg gaauuugugu acgggaugua ucggcaaaag     3060

aaacuuagca agauuguuca cgaggacaaa gaagagaagu acucugaggc cacgagaaaa     3120

auguucuuuu auagcaaccu gaugaauaug uucaagcgcg ugguccggcu cgcugaugga     3180

uccaucgugg uuagacccgu gauugaaaca ggccgcuaca ugcggaaaac cgccugggac     3240

aagaaaaagc auuucgccac agucagaaag gugcugagcu auccucagaa caauaucguu     3300

aaaaagaccg agauccagac cgggggauuu ucuaaagaau ccauucucgc ucacggcaac     3360

agcgacaagc ugaucccccg caaaacaaag gauauuuacc uugacccuaa gaaauauggg     3420

ggcuucgauu cccccaucgu ggccuacucu guccuggugg uggcggacau uaagaaagga     3480

aaggcucaga aacucaagac ggucaccgag cugcugggca ucacaaucau ggaacggagc     3540

agauuugaga agaauccguc cgccuuccuu gagucuaaag gguaccugaa cauucgggau     3600

gacaagcuca ugauccugcc uaaauauagc cucuuugaac uggagaaugg acgcagacgg     3660

cuucuggccu ccgcuggcga acugcaaaag gggaacgagc ucgcccugcc cacccaguuc     3720

augaaauuuc uuuaccuggc gucucgcuau aaugagagca agggaaagcc ugaagagauu     3780

gaaaagaaac aggaguucgu uaaccagcau guguccuacu uugaugacau ccuccaacug     3840

auuaaugauu ucagcaaaag agugauccuc gcugacgcca accuggaaaa gauuaauaaa     3900

cuuuaucagg auaacaagga gaacaucccc gucgacgagc uggccaauaa caucauuaau     3960

cuguuuacau ucaccucucu cggcgcuccu gccgcuuuua aauucuucga uaagaucgug     4020

gaccggaaga gauacacguc cacaaaagaa guucugaaca gcacccuuau ucaccagucu     4080

aucaccgggc uguaugagac acgcauugac cucggcaagc ugggagaaga uuga           4134


<210>  4350
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4350
auggagaaga ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

aucacggaug acuauaaagu ucccgcuaag aaaaugaagg ugcucggcaa uaccgauaaa      120

aaguacauua agaaaaaccu gcuuggggcc cuccuguucg acucuggaga aacagcggag      180

gcuaccagac ugaagcgggc cgcccgcaga cgguauacac gcagaaagaa ucggcuucgc      240

uaccugcagg aaaucuuugc uaaggagaug gccaaagugg augaaucuuu cuuucaaaga      300

cucgaggagu ccuuccugac cgacgaugac aagacauuug auucucaccc uauuuucggc      360

aacaaggcug aagaggacac cuaucaucag gaauuuccga cgaucuacca ucugcggaaa      420

caccucgccg auagccccga gaaggucgac cugcgccuug uguaucuggc gcucgcccac      480

augaucaaau ucagagggca uuuucugauu gagggacagc uuaaugcuga aaacaccgau      540

guucagaaga ucuucgccga cuuugugggc gucuacgacc ggacauucga ugacucccac      600

cugucugaga uuaccgugga ugcugccagc auccucacag agaaaauuuc caagagccgc      660

agacuggaga aucugaucaa acaauacccu accgaaaaga agaacacgcu cuuuggcaau      720

cugguugcgc uugcccuggg gcuccagccc aacuucaaaa caaauuucaa gcugucugag      780

gacgcuaaac uucaguuuuc caaggauacc uaugacgagg aucuggagga gcuccuggga      840

aaaauuggcg acgauuacgc cgaccuguuc acagcugcca agaaccucua ugaugccauc      900

cugcuuagcg ggauccugac cguggacgac aauucuacca aggcuccucu cuccgcgagc      960

augauuaaac gguacgagga acaccaugaa gaucuggaga aacuuaaaac auuuaucaag     1020

gucaacaauu ucgacaaaua ucacgagauu uuuaaggaua agucuaaaaa cggauacgcc     1080

ggcuauaucg aaaacggggu gaagcaggac auuuucuaca aacaucugaa guccaucauc     1140

agcgagaaaa auggaggcca auacuuucuc gauaagauug aacgcgagga cuuccugaga     1200

aagcagcgga cguuugauaa cggcuccauc cccuaucaga uucaucugca ggaaaugaga     1260

accauccucc gccggcaagg ggaguacuau ccuuuccuga aagagaauca ggcuaagauu     1320

gagaaaaucc uuacauuuag aaucccguac uaugugggac cccuggcccg caagaacucu     1380

cgguucgccu gggcuaaaua ccacagcgac gaaccuauua cccccuggaa uuuugaugaa     1440

gucguugaca aggagaaauc cgccgagaag uucaucacaa gaaugacccu caacgaucug     1500

uaucugccug aagagaaggu gcuucccaaa cacagcuacg uguacgaaac guucgcgguc     1560

uauaaugagc ucacaaagau uaaauacgug aacgaacagg gcaagagcuu uuucuuugac     1620

gcuaauauga aacaagagau cuucgaucac guuuuuaagg agaaccgcaa ggugaccaaa     1680

gccaagcugc uguccuaucu caauaacgag uucgaagaau uucggauuaa ugaccugauc     1740

gggcuugaca aagauagcaa gucuuucaac gccagccugg gaaccuacca ugaccucaaa     1800

aagauucugg auaaguccuu ucuggacgau aaaacaaaug agcagaucau cgaagacauu     1860

guccuuaccc ucacacuguu cgaggauaga gacaugaucc acgagcgccu gcagaaguau     1920

ucugauauuu uuacgagcca gcaacucaaa aagcuggaac ggagacauua caccggcugg     1980

gggcgccuuu ccuauaaacu gaucaacggc auucggaaca aggagaauaa caagacaauc     2040

cucgacuucc ugaucgauga cggagaugcu aauagaaacu uuaugcagcu gauuaaugac     2100

gacagccuuu cuuucaaaac cacaauccag gaagcccagg ugguuggcga uguggacgau     2160

auugaggcug ucgugcacga ccucccgggg uccccugcca ucaagaaagg cauucugcaa     2220

agcgucaaga ucguggauga acugguuaaa gugaugggac acaaccccca gaauaucguc     2280

auugagaugg cgcgggagaa ccagaucacc ggguacggac gcaauagauc uaaccagcgg     2340

cucaagagac ugcaagacuc ccuuaaagag uucggcagcg auauucuguc caagaagaaa     2400

ccuucuuacg uggacagcaa gguugaaaau ucccaucucc agaacgaucg ccuguuucug     2460

uauuacaucc agaaugggaa agacauguau acgggcgagg aacuugauau ugaccaccuc     2520

ucugacuacg auaucgacca uaucauuccc caggccuuca ucaaggauaa cagcauugac     2580

aaucgggugc ugaccuccag cgcuaaaaac agaggaaagu cugaugacgu ccccuccauc     2640

gaaauugugc gcaacagaaa gagcuauugg uacaaacugu auaagucugg ccucaucucc     2700

aaacggaagu uugauaaucu gacaaaagcc gaacgcgggg gacuuaccga gacagacaag     2760

gcuggcuuca uuaagagaca acugguggaa acccggcaga ucacgaaaca cguugcccag     2820

auccucgaug cgcgcuuuaa cacaaagugc gacgagaaug auaaagucau uagagacgug     2880

aaggugauca cccugaaaag cucucugguc agccaauucc ggaaggaauu uaaguucuac     2940

aaagugcgcg agauuaacga uuaccaccau gcccacgacg cuuaucuuaa ugccguugug     3000

gggacagcuc ugcucaagaa auacccuaag cuggccccgg aguuugucua uggagaauac     3060

aaaaaguaug acgugagaaa gcucguugcc aaauccagcg auaaccauuc ugagcugggc     3120

aaggcuaccg cgaaauacuu cuuuuauucc aaucuuauga acuucuucaa gaccgaagug     3180

aaauacgccg acgggcgggu cuuugagcgc cccgauaucg aaacaaaugc ugacggcgag     3240

gugguuugga acaagcagag agauuucaau auugugcgga aaguccugag cuacccucag     3300

gugaacaucg ugaagaaggu cgagguucag acgggaggcu uuuccaaaga aucuauccuc     3360

cccaaggggg acagcgauaa acugauuccu agaaagacca aaaagcugca augggagaca     3420

cagaaguaug gaggcuucga cucccccacc guggccuacu cugugcuugu cguggccgau     3480

guugaaaaag ggaagacacg caaacugaag accgugaaag agcucgucgg aaucagcauu     3540

auggaacggu ccucuuuuga ggagaauccg gugagcuucc uggaaaagaa gggcuaucac     3600

aacguucagg aggacaaacu caucaagcug ccuaaauacu cccuuuuuga guucgaaggg     3660

ggcagacgcc ggcugcucgc uagcgccacg gaacugcaga agggaaauga agugguccug     3720

ccccaauaua uggugaaccu ucuguaccau ucucagcacg uuaacaauuc ccacaaaccu     3780

gagcaucuca acuaugugaa gcagcacaag gaugaauuua aagacauuuu caaucugauc     3840

aucagcauug cgagaaucaa cauucucaag cccaaagucg uggauaaucu gaucaacgag     3900

uuuacagaau acggccagga ggacauuucu ucccuuagcg agucuuucau caaucugcug     3960

aaguuuauuu ccuucggggc uccuggagcc uucaaauuuc ucaagcugga cgugaaacaa     4020

agcaaccuuc gcuacaaguc caccaccgaa gcccugagcg cuacacucau ccaucagucu     4080

gucaccggcc uguaugagac acggaucgau cucagcaagc ugggggaaga guga           4134


<210>  4351
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4351
augaagaccu ucgccgagga auacgugaug gacaaaagca agcccuaugg caucgggcug       60

gauauuggaa caaacuccgu cggcuuuguu gcuacggacg ccgaggggca ccucaucaga      120

cugaaaggaa agaccgugau uggcgcguac cuuuucaaug cugggaucuc ugccgccgaa      180

cggcgcggau uuagaacaac ccggcgcaga cugagccggg ugaaauggcg ccucggccug      240

cuuagagaga uuuucgaaac acauuuucag gaguccaugg gcgagaacga agauaaugac      300

uucuuucugc gguucaagua uucuaacauc agcccuaagg auccgcaauu uuccaccgcu      360

aaaggacucu ucaaugaccg cacagauaag gaguuuuacg accaguaucc caccaucuac      420

caccugagac gggcccugau gacggaagau caucaguucg acauucgcga gaucuauauu      480

gcuaugcacc auaucgucaa auacagaggg cacuuucuca aggagggccg ggccaaagau      540

uucaaggugg gggaccugcg ccuucuggau aacuucaaga ugaugaauga acagauugag      600

gaaaucaacc cucucuggca acugaaacuu cccaccgacg acgcgucuau uaagagcauc      660

acagccaucc ugcucgauaa uacccagucc cagaacgaca gacagaaagc uguuacaaag      720

gugauucugg ccacccuggu caaagcuagc gauaaggaca ucaaugccgc gcggaaaaga      780

uuugugggag agcucucuaa ggccaugguu ggccugaaga cgaaacuuug ggugcuggcu      840

gaugucuccc aaaacgggga cugggaaauu aaguacgaga auuaugccga uuucgcugag      900

acaaucggaa gcggcgaauc ugacaccauu cagucccucu uuaacgagau caaugaucug      960

uacggcguga ucacacuugc cgggauuauc ccuaaagaag ccgagagcuu cucugacgga     1020

auggugcgca aguaugaaca ccaucggaaa aaccuggagc uccugaaggu cuacugcgcu     1080

gagcaguccg auggcaagag agggcgccag auucggcaaa ccuaugacaa auacaucgac     1140

ggaguugaua gcaagcaguu uacccaggaa gacuucuaua aagcgcuguc caaguuuaca     1200

gccaaagaug aggcuacguc ugaaaacgcc aagcucauug cccaagagau cgcugugggc     1260

accuucaugc ccaagcugag aacaaaagcc aaugggacca ucccucacca gcuucaucag     1320

aaggaacugg acgcgauuau cgagaaccag aaaaaguacu acccguggcu cggagaggug     1380

aaucccgucg aaagccaccg ccgggcucug ccuuauaaac uugaugagcu gguguccuuu     1440

cgcauucccu acuauguugg cccuauggug acacccacca agggcgaccc ggaaaagagc     1500

aaauucgccu ggauggucag aaaggagccu gggacgauca cacccuggaa cuuugaucaa     1560

aaaguggacc ggucugccuc cggagaggcu uucauucaga gaaugaagac caccgauaca     1620

uuucucaucg gcgaagacgu ucugccucag cagagccugc ucuaccaaaa auucgaggug     1680

cugaaugaac uuaacaagau uaugaucaau gggaaaccca ucugucgcga gcagaagcag     1740

cggcuguuca agcagcucuu uaugcaauau aaaaccguca cagugaagaa aguccagcag     1800

aaccugauug ccaauggaga agagucugag aacgugccca ucacgggccu gagcgauccu     1860

cuuagauuca auuccucuuu uagcaccuac auugacuaua aggauauccu cgggacagcu     1920

gccguuaacg acaaugcgaa acaguccgac auugaacaaa ucaucgccug gagcaccauu     1980

uucgaggaug cugccaucuu ucgggaaaag cugaacgaca uuacauggcu gaaugaugac     2040

cagcgcaaca agcucucuca caaaagauac cggggcuggg gaagacauuc ccgcaagcug     2100

cuugcuggcc ugcgggaugg ggagggacag accaucauug aaagacucug gaacacgaau     2160

gacaacuuca ugcaaaucca gaaugauagc gagaucgccc gccagauuac cgaggcgaac     2220

ucuuccaaaa uggccacagc ugaaggcacc gacgagauca uugauggguu uuacacaagc     2280

ccggaaaaua agaaagcccu gagagaggug augaaggucg ugaaggacau ccagcgggcu     2340

caccauggac aagcccccgc cuggguuuau auugaauccc cucgcgagac ccccagaccu     2400

ggccagcgga cggugucucg cgagcagcag cugacagauc uuuacgaagg ggcugcgaaa     2460

gagaucgucg acgaugccgu gcucaacgaa cugaaggaca aaguuaagag caaagagaau     2520

uucaccgaca agcuggugcu cuauuuucug caaaacggcc acgauaucua cgcuaaugac     2580

uccauuaaca ucgauaaucu uaacgccuau gacauugauc auguccugcc ccagucucuc     2640

aucaaggacg auagccugga caauagagug cugacaaccc acgagcggaa ccuuaaaaag     2700

uccaaucgcu ucgccaccga acucuuugcu gaucagagaa agaaguggga gaaauggcac     2760

cggcugggac ugauuagcuc ucgcaagcuc aaacaucuga caaugcagcc gaacuccgug     2820

gaaaaguucg cccacggcuu uaucgcgaga caacuuacgg agacccggca gauuauccau     2880

cugacagcua acguccucag caaucuguac caggaaaacg acaccaagau cgugaugauu     2940

aaagccgggc uuaauucuga guucagacgc acauucgauu uuccuaagaa ccgguccguu     3000

aaugacuauc accacgccuu cgacgcuuuu cuuaccgcca aaaucggaag auaccuccug     3060

gcgcgcuacc ccaagcugga gccuuucuuu guguauggca acuucgucaa aaaucccaag     3120

gcuaugaagc ggcucagcuc uuuugauuuc auugcccaac uggccgcuaa aacggacgau     3180

acaagccaua ucgaccagag aucccuuaaa caggugccug uugugaacga agagaccggg     3240

gaaauugucu gggauaaaga caucgagcug gccgaacucg auaagaccua caauuauaaa     3300

acaaugcugg ugaagcgcgc ucagaccgag aacaaugccc aaauguuuaa gcagacaguu     3360

uucaaagcgc gggacaacca gaauaagacg cugauccccg ugaaaaacgg acuuagcacc     3420

gaugucuacg gcgggcacuc ucagcaagcc auuuccuauc ugugcaucgu gugggugggc     3480

cagaagaaaa aguacagagu ccucggaauu agcacagcuc augccggcau ccugaauaac     3540

uuugagaaga acuaugggcg cuucgaagcu aaaaagaaac uccaggagau uguuuccaau     3600

acccuggaca acgccgaucg gaaugacuuc aagaucgugg ccccgaaagu gcuuuuugaa     3660

caggucgugg aggaugacaa caugaaguuc ggacugggcu cugcuagcga cuacagaaau     3720

guucaacagc ucuuucuguc ccggaagaac cagcugcuuc uggcgaauau gaugacagau     3780

cagauccacg accaagaucu cgugcaucug uucgacgaaa uugucgggca gaugaacgcc     3840

cacuuuccua ucuucgaucg cggaggcuac agaucuagcc ucacccaguc ccgggacaaa     3900

uuucugaagc ugcccuucaa aaagaaugag gaucugauua cgaaacaaga ggugauccgc     3960

cggauucucg acgggcugca cgcuaacgcc aauagaaagg aucugaaaau cauuggcucu     4020

aagggagacu uuggcagacu ugggaccaag aaaaucuauc ugagcaagga ugccaaacuc     4080

aucuacacau cccccaccug ucuguucaca cgcaccguuc cucucagcuc ucuguga        4137


<210>  4352
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4352
augaccaacg agaaguacag caucggccug gacauuggga cauccucuau cggauucgcc       60

guggucaaug auaacaauag aguuauucgg gugaaaggca agaacgcuau cggggugcgc      120

cucuuugacg aaggaaaagc cgcggcugau agacggagcu uccgcacgac cagacggcgc      180

cuguccagac ggcgcuggag acuuaagcug cuccgggaga uuuuugacgc cuauaucaca      240

cccgucgaug aagccuucuu uauccgccug aaagagucua aucuuagccc uaaggacucc      300

aagaaacagu acucuggcga uauucuguuc aacgacagaa gcgauaagga cuuuuaugaa      360

aaauacccga ccaucuauca ccuccggaau gcucugauga cagagcaucg caaguucgau      420

gugagagaga uuuaccuggc cauccaccau auuaugaaau uucgggggca cuuccucaac      480

gcuacccccg ccaauaacuu uaagguugga agacugaauc uugaagagaa guucgaggaa      540

cugaacgaca ucuaucaacg cguguuuccu gaugagucca uugaauuucg gacagacaau      600

cucgagcaga ucaaagaagu ccugcuugac aacaagagaa gccgcgcgga ucggcagaga      660

acccuggugu cugacaucua ccaguccagc gaggauaaag acauugaaaa gcgcaauaaa      720

gccguugcua cggagauccu caaggccucu cugggcaaca aagcuaagcu gaacgugauu      780

accaaugucg agguggauaa ggaagccgcg aaagaguggu ccaucacauu cgacagcgaa      840

ucuauugaug acgaucucgc caagaucgag ggccaaauga ccgacgaugg gcaugaaauc      900

auugaggugc ugcggucccu uuacagcgga aucacacugu ccgcuauugu ccccgagaac      960

cacacccucu cucagagcau gguugccaaa uaugaccugc acaaggacca ucuuaaacug     1020

uuuaagaagc ucaucaaugg caugacggau acaaagaagg cuaaaaaccu gagagccgcc     1080

uacgacgggu auauugaugg agugaagggc aaagugcugc cucaggaaga cuucuacaag     1140

cagguccaag ugaaucucga ugacuccgcu gaggcgaacg aaauccagac cuauaucgau     1200

caggacauuu uuaugcccaa gcagcgcaca aaagccaaug ggagcauccc ucaccaacug     1260

cagcagcaag agcuugauca gauuaucgaa aaccagaagg cuuacuaucc guggcuggcc     1320

gagcucaauc ccaacccuga caaaaagcgg cagcaacugg ccaaauacaa acuugaugag     1380

cugguuaccu ucagagugcc cuacuauguc ggaccuauga uuaccgcuaa ggaccagaaa     1440

aaucagucug gcgccgaauu ugcguggaug auccgcaagg agcccggcaa cauuacaccg     1500

uggaauuucg aucagaaagu ggaccggaug gcuacggcca accaauuuau caagagaaug     1560

accacaaccg acacauaccu ccugggggaa gauguucugc cugcccaguc ccuccuguau     1620

cagaaauucg aggugcuuaa ugagcugaac aagauccgga uugaccauaa acccaucagc     1680

auugaacaga agcaacagau cuuuaacgau cucuucaagc aguuuaaaaa ugucaccauu     1740

aagcaucugc aggacuaccu ggugucucaa ggacaguaua gcaaacgccc ucuuaucgag     1800

ggccucgcug augaaaagag auucaacucc ucucugagca cguacuccga ccugugcggg     1860

aucuuuggag ccaaacucgu cgaggaaaau gaucggcagg aggaccugga gaagauuauc     1920

gaauggagca caauuuucga ggauaagaaa aucuauagag cuaaacuuaa cgaccugacc     1980

uggcucaccg augaccaaaa agaaaagcug gccacaaaac gcuaccaggg cugggggcgg     2040

cugucuagaa aacuucucgu gggacugaaa aauuccgagc accgcaacau uauggauauc     2100

cuguggauca ccaaugaaaa cuucaugcag auucaggcgg agcccgacuu ugccaagcuc     2160

guuacagacg cuaauaaggg caugcuggag aagacggaua gccaagacgu gaucaacgau     2220

cuuuacaccu cuccccagaa uaagaaagcc auuagacaga uccugcucgu cgugcacgac     2280

auucagaacg cuaugcacgg ccaagccccu gcgaagaucc auguugaguu cgcccgcggg     2340

gaagaacgga auccgagacg guccgugcag cgccagagac aggucgaggc ugccuaugaa     2400

aaggugagca acgagcuggu guccgcuaaa guucggcaag aguuuaagga agccaucaau     2460

aacaaacgcg auuucaagga cagacugcuu cucuacuuua ugcagggagg cauugauauc     2520

uauacaggga aacagcugaa cauugaccaa cugucuagcu accagaucga ucacauucuc     2580

ccccaggccu ucgucaagga cgauucccug accaaucggg ugcuuacaaa cgagaaucag     2640

gugaaagcug acucuguccc uaucgacauu uuuggaaaga agaugcugag cguguggggc     2700

cgcaugaaag aucaagggcu caucuccaag ggcaaauaua gaaaccugac caugaauccc     2760

gaaaacauca gcgcgcauac ggagaaugga uucauuaacc ggcagcuggu ugagaccaga     2820

caggugauca aacuugccgu caauauucuc gcugacgaau acggcgauuc uacacagauc     2880

auuuccguga aggccgaccu gagccaccaa augcgcgagg auuuugaacu gcucaagaac     2940

cgggacguua augauuauca ccaugccuuc gacgcuuacc uggccgcguu uaucgggaac     3000

uaccuucuga agagauaucc uaaacucgag ucuuacuucg uguauggaga uuuuaagaaa     3060

uucacccaga aggaaacaaa aaugcgccgg uucaauuuua ucuacgaccu gaagcacugu     3120

gaucaggucg ugaacaagga gaccggcgag auucugugga cgaaagacga agauaucaag     3180

uauauuagac aucuuuucgc uuacaaaaag auccugguuu cccacgaggu gcgcgaaaaa     3240

cggggagccc ucuauaauca gacaauuuac aaggccaagg acgacaaagg gagcggccaa     3300

gggucuaaga aacugaucag aaucaaggau gacaaagaga ccaagauuua cggcggauau     3360

agcggcaagu cccucgcuua caugacaauc guccagauua ccaaaaagaa caaagugagc     3420

uaucgcguga ucgggauucc cacccuggcc cuugcgcggc ugaacaagcu cgaaaaugau     3480

ucuacagaga acaauggaga gcuguacaaa aucauuaagc cgcaguuuac gcacuauaaa     3540

gucgacaaga agaacggcga aaucaucgag accacagaug acuucaaaau uguugugucc     3600

aaggugagau uucagcaacu gaucgaugac gcugggcagu ucuuuaugcu ugccagcgau     3660

accuacaaaa auaacgccca gcaacugguc auuuccaaua acgcucucaa ggccaucaau     3720

aacacaaaua uuaccgacug cccucgggau gaccuggaac gccucgauaa ccugagacuu     3780

gacucugcuu ucgacgagau cgugaaaaag auggauaagu auuuuagcgc cuacgacgcg     3840

aauaacuucc gggaaaagau ccgcaauucc aaccugauuu ucuaccagcu ccccguugag     3900

gaucaguggg agaauaacaa gaucacggaa cugggaaaaa gaacagugcu gacccggauu     3960

cuucagggcc ugcaugccaa cgcuaccaca accgacaugu cuaucuucaa gauuaaaaca     4020

ccuuucgggc aacucagaca gcgcagcggc aucucccugu cugagaaugc ccagcucauc     4080

uaucagagcc ccacgggacu guuugaacgg agaguccaac uuaacaagau uaaguga        4137


<210>  4353
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4353
augaagaaca aagacuacag caucggccug gauauuggga ccuccucuau cggauucgcc       60

guggucgacg agaauaacca cguuaugaga gugaagggca aaaaugcuau uggggugcgg      120

cucuuugaug aaggaaagcc cgccgcggac cgcagagcuu uccggacaac gcgcagacgg      180

cugagccgca gacgguggcg ccuuaaacug cucagagaga ucuuugauuc cuauauuacc      240

ccugucgacg aagccuucuu uauccggcug aaggagucua accuuagccc gaaggauucc      300

aaaaagcagu acucuggcga cauccuguuc aaugaucgca gcgacaaaga uuuuuauaca      360

aaguacccca ccauuuauca ucucagaaac gcccugauga cagaacacca acgguucgac      420

auccgcgaga uuuaccuggc uauccaucac auugugaaau uuagagggca uuuccucaau      480

gccaccccug cuaacaauuu ucagguugga aagcuggauc uugaccagaa guucgaggac      540

cugaacgaua ucuaucagcg gguguuuccc gacgauucca uugaguucag aaucgacaau      600

cucgaacaaa ucaaagccgu ccugcuugau agcaagcgcu cucgguccga cagacagcgc      660

agccuggugg augacguuua ccagacaucu gaagauaaag agguggagaa gcagaacaaa      720

gcggucgcca ccgaagugcu caaggcuauu cugggcaaua aagccaagcu gaacaucauu      780

gcuaaugugg aggucgacaa ggaagccgcg aaagagugga cgaucaccuu cgauuccgaa      840

agcauugacg acgaucucgc caagaucgac ggccaaauga cagaugacgg gcgggcuauc      900

guugagauuc ugagaucucu uuacuccgga aucacccuga gcgccauugu gccugagaac      960

cacacacucu cccaggcuau gguggccaaa uaugaucugc acaagaaaca ucuuacccug     1020

cucaagaagc ugaucaacuc uaugacggac acaaagaagg ccaaaaaucu gcgcgcugcg     1080

uacgauggcu augucgacgg ggugaaggga aaaguucuca cccaggauga auuuuacaag     1140

cagauucaaa gcaaccugga cgaguccgug gaagccagcg agauccagac auauaucgau     1200

caggacauuu ucaugcccaa gcaacggacc aaagcuaaug gcucuauccc ucaccagcuu     1260

cagcagcaag aacuggauca gauuaucgag aaccagaagg aguacuaucc guggcucgcc     1320

gaacugaauc ccaacccuga caaaaagaga cagcaacuug ccaaauacaa gcuggacgag     1380

cucgucaccu uucgcgugcc cuacuauguu gggccuauga uuacagcuga agcccagaag     1440

aaacaguccg gagcggaguu cgcuuggaug auccggaagg aaagcggcaa uauuacgccc     1500

uggaacuuug aucagaaagu ggacagaaug gccaccgcca aucaauucau caagcgcaug     1560

acaaccacag auaccuaccu gcugggcgag gacguccucc cgucucagag ccugcuuuau     1620

cagcgguuug aggugcugaa cgaacucaau aaaaucagaa uugaucggaa gccuaucucc     1680

gucgagcuga aacagcaaau uuucaacgac cuguuuaaga aguucaaaaa ugugacgauc     1740

aagcaucuuc aggauuaccu cguugcucag gggcaauauu cuaaacgccc ccugauugaa     1800

ggacuggccg acgagaagag auuuaacagc ggccucucca cauacaauga uuucugcggg     1860

aucuuuggag cuaaacuggu ggacgagaac gaucggcagg ccgaccuuga aaagaucauu     1920

gaguggagca ccaucuucga agauaagaaa auuuauagag cgaagcugaa cgaccucgcc     1980

uggcugaccg acgagcagaa agauaagcug gcuacaaaac gcuaccaggg cugggggcgg     2040

cuuucuucca agcuccugau cggacugcaa aauagcgaac acagaaacau uauggaccuc     2100

cuguggaucg gcaaugagaa cuucaugcag auccaggccg auucugacuu ugcuaaacuu     2160

auuaaagaug ccaaucagaa cgcgcugcaa aauaacgacu cccaggaugu caucaaugac     2220

cucuacacca gcccucagaa caagaaagcc auucgccaga uccugcuggu gguucacgau     2280

auucaaaaug cuaugcaugg ccaggccccc acaaagaucc acguggaguu cgcuaaaggg     2340

gaagagagaa acccccggcg cuccguccag agacaacgcc agguggaagc cgccuaugag     2400

aaggcuucua augaacuugu gggagcgaac guucgggagg agcucaagga cgccaucacg     2460

aauaaaagag auuuuaagga ccggcuguuc cuguacuuua ugcagggcgg ggucgacaug     2520

uauagcggag aaccuauuaa caucgauaac cucucccgcu acgacauuga ucauauccug     2580

ccgcaggcuu ucgugaaaga cgauucucuu aauaacagag ugcugaccga cgccgagaag     2640

aaucggcgca aaagcgaugc cguccccaac gaacucuuug gcgcuaagau gagagugaca     2700

ugggagcgga ugaaagaaca agggcugauu uccaagggca aguauagaaa ucugaccaug     2760

aacccugaga auaucaacaa auacacagag aauggauuca uuaaccgcca gcuuguugaa     2820

accagacagg ugaucaagcu cgccgucaau auccuggcgg acgaguauaa agaaaacacg     2880

cagauuauca gcgugaaggc ugaucugucu caccaaaugc gggaggacuu ugagcuccug     2940

aaaaaucgcg auguuaacga cuaccaucac gccuucgaug ccuaccuugc ugccuuuaug     3000

ggcaauuauc ugcucaagcg guaccccaag cugagauccu auuucgugua cggggacuuu     3060

aaaaaguuca cccagaaaga aauuaagcug cgccgguuca acuuuaucua ugaccuuaaa     3120

cacgaggauc aggucauuga caaggaaaca ggagagaucc uguggaacaa gaaugaagau     3180

aucgcguaca uuagacaucu cuucgcuuau aaaaagaucc ugauuagcca cgaggugucu     3240

gagcgccggg gcgcccucua caaccagacc guuuacaaag ccuccgacga uaaggcuagc     3300

gggcaaggau cuaaaaagcu gaucccuauu aagagcgaca aacccacaga aaucuauggc     3360

ggguacaccg gcagaacgcu ugccuauaug acaaucguga aggucaccaa aggaaagaaa     3420

auuuccuaca aggugguggg cgucccgaca gcuguucugg ccaagauggc gggggugcgg     3480

cagggaagcc aggaggaaca ggauaaacuc uauggcgugc ugaagccuca auuuacccau     3540

cagaaagaga auaagaaaac cggggaaauc acagagacgg ucgacaaguu cgauauuauc     3600

gugcccaaag uuaaguacca gcaacuggug aacgacaaug gacagcuuuu uaugauuggc     3660

ucugauaccc ugccuuauaa cgccgagcag cucguccugu ccaaggaaag cgugcagaca     3720

aucaauaacc ccgggauuaa agacgagggc uccgaugaag agucugaaaa uaagaaacuc     3780

gacaacguuu acgacgagau cgugcugugu augaauaagu acuucccucu uuaugauaac     3840

cgcggauuua gaaauagcau caccaacggc aaaaauaagu uccugaagcu ccccauuaaa     3900

aacaucuaca agaaugggaa acugauuaac gucggaaagc gggaggugau caaccgcauu     3960

cugcaaggcc uucacgcuaa ugccuccguu acagaccugu cuaaccucgg gaucaaaagc     4020

ccguuuggau uccugcagaa gggcgcuggg cucucccugu cuggcgaagc ccagcuuauc     4080

caccagagcg ccaccggacu guuugagcau agagugcaac ugaauaagau uaaauga        4137


<210>  4354
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4354
augagcaaca agcccuacuc caucggccug gacauuggga ccaauucugu gggaugggcc       60

gucaucacag augacuauaa aguuccuagc aagaaaauga aggugcucgg caacacggau      120

aaacacuuca uuaagaagaa ucugaucggg gcccuucugu uugacgaggg aaccacagcu      180

gaaggcagac ggcucaaacg caccgcgaga cggcgcuaca caagacggaa gaaccgccug      240

agauaucuuc aggagauuuu cuccgaagag aucucuaaag uggauagcuc cuuuuuccau      300

cggcuggacg auucuuuucu cgucccggaa gacaagcgcg ggagcaaaua ccccaucuuc      360

gcuacccugg ccgaggagaa ggaauaucac aagaauuuuc cuacaauuua ccaucugaga      420

aaacaacucg ccgauuccaa ggagaaagcu gaccugcggc uuaucuaucu ggcccucgcu      480

cacaugauga aguaccgcgg acauuuccug uacgaagaga gcuuugauau uaaaaacaau      540

gacauccaga agauuuucaa cgaguuuauc ucuauuuaug auaauaccuu cgaaggcucc      600

agccuuucug agcagaacgu gcagguugaa gccaucuuua cggacaaaau cuccaagagc      660

gcgaagagag agcgggugcu gaaacucuuc cccgacgaaa agucuaccgg ccuguucucc      720

gaguuucuga aacucauugu cgggaaucaa gccgaguuca agaaacacuu ugaucuggaa      780

gagaaggcuc cucuucaguu cagcaaggac acauacgaug aagaccugga gaaccuccug      840

ggacagaucg gcgauggguu ugccgaccuu uuccuggugg cuaaaaagcu cuaugaugcc      900

auucugcugu ccggaauccu caccguuaca gaccccucua ccaaagcgcc ucugagcgcc      960

uccaugauug aacgcuacga gaaucaccag aaggaucuug cuagccugaa acaauuuauc     1020

aagaacaauc ucccggccga gaagugcgug gaagucuucu cugaccaguc caaagauggc     1080

uaugcugggu acaucgacgg aaagacgaca caggaggccu uuuauaaaua cauuaagaac     1140

cugcuuagca aacuggaagg cgccuacuau uuccucgaca agaucgagag agaagauuuu     1200

cugcggaagc agagaaccuu cgacaacggc ucuauucccc aucaaaucca ucugcaggag     1260

augaaugcua uucuccgccg gcagggggag cacuacccuu uucugaaaga aaacagagag     1320

aagaucgaaa agauccuuac auuccgcauu cccuaucacg ugggaccucu ggcgcggggc     1380

aauagagauu ucgccuggcu cacccgcaac agcgacgagg cuauccggcc cuggaauuuu     1440

gaggaaauug uggaucaagc cuccucugcc gaggacuuca ucaacaagau gaccaauuac     1500

gaucuguauc ugccggaaga gaaaguccuu ccuaagcaca gccuccugua cgaaacauuu     1560

gcuguuuaua acgagcugac gaaagugaag uucauugccg aggggcucac cagauaccag     1620

uuucuggaca agaaacagaa gaaagauauc uucuacacau uuuucaaggc ggaagacaaa     1680

cgcaagguga ccgagaagga uauuauccau uaucuucaca augucgacgg auacgauggc     1740

aucgaacuga aagggauuga gaagcaguuu aacgcuuccc ucagcacaua ucaugaccug     1800

cugaaaauca uuaaggacaa agaauuuaug gaugacccca agaaugagga gauccuugaa     1860

aacauugugc acacccucac gaucuuugag gaucgggaaa ugaucaagca aagacuggcc     1920

caguacgacu cucuguucga ugagaaaguu auuaaggccc ucacacgccg gcauuauacc     1980

ggauggggca aacuguccgc uaaacuuauc aauggcauuu gugacaaaca gaccgggaag     2040

acaauccugg auuaccucau ugacgaugga aagaucaaca gaaauuuuau gcagcugauc     2100

aacgacgaug gccugagcuu caaagaaauu auccaaaagg cccagguggu cgggaaaacc     2160

uaugacguga agcagguugu gcaggagcuu ccuggaucuc ccgcuauuaa aaagggcauc     2220

cuccaauccg ucaaacuggu ggaugagcug gucaagguga uggggcacgc ccccgaaagc     2280

auuguuaucg agauggcgcg ggaaaaccag acaacggcca agggcaaaaa gaacucccag     2340

cagcgcuaca aaagaaucga ggacgcuauu aagaaucucg ccccuggacu ggacucuacc     2400

auccuuaaag agcacccgac agauaauauu caacugcaga acgacagacu cuuccuguac     2460

uaucugcaga auggccggga uauguacacc gggaagcccc uugaaaucaa ccaacucagc     2520

aauuaugaca uugaucauau cauuccucag gcuuuuauca aggacgauuc ccuggacaac     2580

cgcgugcuga caucuagcaa agagaaucgg ggaaaguccg auaacguccc cagccucgaa     2640

gugguugaga aaaugaaggc cuucuggcag cagcugcuug acucuaaacu gaucuccgaa     2700

agaaaguuua auaaccucac caaggcggag cgcggcgggc uggaugagcu ggacaaagug     2760

ggauucauua agagacaacu ugucgaaacg cggcagauca ccaaacacgu ggcccagauu     2820

cuggacgcuc gcuuuaauaa ggaggugaca gagaaaaaca agaagaauag aaacguuaaa     2880

aucauuaccc ucaagagcaa cauggucucu aauuuccgga aagaauuugg ccuguacaag     2940

gugcgcgaaa ucaacgauua ucaucacgcc cacgacgcuu accucaaugc cguggucgcc     3000

aaagcuaucc ugaagaagua uccuaaacuu gagcccgagu ucguguacgg ggauuaccag     3060

aaguaugacc ugaaaagaua cauuuccaag agcaaagauc cgaaggaagu ugagaaagcg     3120

acagaaaagu auuucuucua cucuaaccuc cugaacuuuu ucaaggagga agugcauuau     3180

gccgacggca ccaucgucaa acgggagaau auugaguaca gcaaggauac gggagaaauc     3240

gcuuggaaua aagagaagga cuucgccaca auuaaaaagg ugcuguccuu uccucaaguu     3300

aacaucguga agaaaaccga ggaacagaca gucggccaga augggggacu uuucgauaac     3360

aauaucguga gcaaggagaa aguuguggac gccucuaagc ugauucccau caaauccggc     3420

cucagcccug aaaaguaugg gggauacgcu cgccccacca uugccuacuc cguccuggug     3480

accuauaagc agaacaauaa aaucaagaac acaauggugg gcauuccugu ccucacgaaa     3540

cuggaguuug aaagagaucc caaucuuuac cugcggaacc ucgggaucaa caauguuucu     3600

cacgugauua agcuggugaa acauaccguc cuggaguuca agcacaagua uggcacauac     3660

cgcagauuua ucgugagcaa ccaagaacuu aaacgggcga aucagaucuu cguuaccaca     3720

aagcagauga aacugauuuc ccaauauacc acgcucgaca agaacgagca ugauucucug     3780

cucaaaaaua accagcagcu gauccagagc auuuggaauc aauuucuuga guucaugcug     3840

gcuacagacc uggugaacaa gaaacaguac gacgaacuca uccugaccaa uaaccagacc     3900

auugaggaau gcagaaauau caugaaccgc gucaucacaa cccuuuccuu uacaaauauu     3960

aacgccggac agaagaagau cauuuucgau gacacgcugu cucucccgau caaacggugg     4020

caauuuaccg auagcgacaa gauggugaaa aagauuaucg aguccacacu gauccaccag     4080

agcauuaccg gccucuauga aacaagaauc gaucugaaua aacuugggga ggacuga        4137


<210>  4355
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4355
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcu cugcuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acaaugcaag aggaaaagua cuaucaugag aaauucccca ccaucuacca ucugcggaag      420

gaacuggcug acaagaaaga gaaggccgau cuccgccuga uuuaccuugc ucuggcccac      480

aucauuaaau uuagaggaca cuuccucauc gaggacgauc gguuugacgu ccgcaacaca      540

gacauucaga agcaguauca ggcguuccug gaaaucuuug auaccacguu cgagaauaac      600

gaccuucugu cccaaaaugu ggauguugaa gccauccuca ccgacaaaau uucuaagagc      660

gcuaaaaagg auagaauccu ggcccgguac ccuaaccaga aguccacagg cauuuuugcu      720

gaguuccuga aacucaucgu gggcaaucag gccgacuuca agaaacauuu uaaccuggaa      780

gauaagacac cccuucaguu cgcgaaagac agcuaugaug aggaccugga gaaucuccug      840

gggcaaauug gagaugaauu ugccgaccuu uucucugcug ccaagaagcu guacgacucc      900

guccuccuga gcggcauccu gaccgugauc gaucucucua ccaaagcucc ucuguccgcc      960

agcaugauuc agagauauga cgagcaccac gaagaucuua agcaucugaa acaguuuguu     1020

aaggccucuc uccccgagaa cuaccgcgaa guguucgcug acuccagcaa agauggguau     1080

gcgggauaca ucgagggcaa gacgucccag gaggccuuuu acaaguaucu gcuuaaacug     1140

cucacaaagc aagaaggguc ugaguaccug cuggaaaaga uuaagaauga ggacuuccuc     1200

cggaaacaga gaaccuuuga uaacggaagc aucccucacc agguccaucu gacagaacuu     1260

cgcgcuauua uccggagaca auccgaguau uacccguucc ugaaggagaa ccucgaccgc     1320

aucgaaaaga uucugaccuu ucggaucccc uauuacgugg gcccucuggc cagagagaaa     1380

agcgauuucg ccuggaugac ccgcaagaca gacgauucua uucggcccug gaauuuugaa     1440

gaccuugugg auaaagagaa guccgcugag gccuucaucc acagaaugac gaacaaugac     1500

cucuaucugc cugaagagaa aguccugccc aagcacagcc ucauuuacga aaaauucacc     1560

guuuacaacg agcugacaaa ggugcgcuuu cuugcggaag gcuucaagga cuuucaguuc     1620

cugaaucgga aacagaagga gaccaucuuu aacucucucu ucaaagagaa gagaaaagug     1680

acagaaaagg auauuaucag cuuucugaau aaggucgacg gguaugaggg aaucgcuauu     1740

aaaggcaucg aaaagcaguu caacgccucc cugucuaccu accaugaucu uaaaaagauu     1800

cucgggaaag acuuucugga uaauacggac aacgagcuga uccucgaaga uauuauccaa     1860

acacugaccc uuuucgagga ccgggagaug aucaagaagc gccuggauau uuauaaagac     1920

uuuuucaccg aaagccagcu caagaaacug uacagacggc acuauacagg auggggcaga     1980

cuguccgcca agcugaucaa ugggauucgc aacaaagaga aucagaagac cauccucgau     2040

uaccugauug acgauggaag cgcuaaccgg aauuuuaugc agcugaucaa ggacgccggc     2100

cucucuuuca aaccgaucau ugacaaggcu agaacaggcu cccauagcga uaaccugaaa     2160

gaagugguug gggagcuugc cggaucuccu gcgaucaaga aaggcauucu gcaaucccuc     2220

aagaucgugg acgaacuggu caaagugaug ggguaugagc ccgagcagau uguuguggaa     2280

auggcccgcg agaaccagac gaccgcuaag ggacugagca gaucccggca gcgccuuaca     2340

acccucagag aaucucuggg caaucugaaa agcaacaucc ucgaggagaa aaagccuaaa     2400

uacgucaagg aucaagugga aaaucaccau cuguccgacg aucggcuuuu ccuguacuau     2460

cuccagaacg ggaaagacau guacacagau gacgagcugg auaucgacaa ucugucucag     2520

uaugauauug accacaucau uccccaggcc uuuaucaagg acgauagcau ugacaaccgc     2580

guccuugugu ccagcgcuaa gaauagaggc aaaucugaug acguucccuc caucgaaauu     2640

gugaaggccc ggaaaauguu cuggaagaac cugcucgaug cgaaacugau gagccaacgc     2700

aaguacgaca aucucaccaa ggccgagaga ggaggccuga cgucugauga caaagcucgg     2760

uuuauccagc gccagcuugu cgaaaccaga caaaucacaa agcacguggc ccggauucug     2820

gaugagagau ucaacaauga gcucgacucc aaagggcgcc ggaucagaaa gguuaaaauu     2880

gugacccuua agagcaaccu ggucucuaau uuucgcaagg aguucggauu uuauaaaauc     2940

cgggaaguga acaauuacca ucacgcucau gaugccuauc uuaacgccgu gguugcuaag     3000

gcgauucuga caaaauaccc ucagcucgaa ccggaguucg ucuacggccu guauagacgc     3060

aagaaacuca gcaagaucgu gcgggaagac aaggaggaga aauacuccga agccaccaga     3120

aagauguuuu ucuauagcaa ccugaugaau auguuuaaac gcgugguccg gcuugcugau     3180

gggucuaucg ugguuagacc cgugauugag acgggacggu acaugggcaa gacagccugg     3240

gacaaaaaga aacacuucgc caccguccgc aaggugcugu ccuauccuca gaacaauauc     3300

guuaagaaaa cagaaauuca gaccgggggc uucagcaagg aguccauccu cgcucacgga     3360

aacucugaca aacugauucc cagaaagacc aaagauaucu accuggaccc uaagaaguau     3420

ggcggguuug auagccccau cguggccuac uccguccuug ugguugcgga cauuaaaaag     3480

ggaaaagcuc aaaagcugaa aacagugacg gaacuccugg gcaucaccau uauggagcgg     3540

ucucgcuucg agaagaauac aagcgccuuu cucgaaucca agggguaccu gaacaucaga     3600

gaugacaaac uuaugauucu gccgaaguau ucucuguucg agcucgaaaa uggacggaga     3660

cgccugcuug ccagcgcugg cgagcugcag aaagggaacg agcucgcccu gccuacccag     3720

uuuaugaagu uccucuaccu ggcgucccgg uauaaugaaa gcaaaggcaa gcccgaggaa     3780

aucgagaaga aacaggaauu ugucaaccaa caugugucuu acuucgauga cauucuucag     3840

cugaucaaug auuuuuccaa cagagugauc cuggcugacg ccaaucucga gaagauuaac     3900

aaacuguauc aggauaauaa ggagaacauc ccugucgacg aacuugccaa caauauuauc     3960

aaccuguuca cauuuaccag ccucggagcu cccgccgcuu ucaaauuuuu cgauaagauu     4020

guugaccgca aacgguacac gucuacaaag gaggugcuua auuccacccu cauccaccag     4080

agcaucaccg gccuguauga aacaagaauu gaccuuggga agcugggaga gggcuga        4137


<210>  4356
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4356
augaccaacg agaaguacag caucggccug gacauuggga cauccucuau cggauucgcc       60

guggucaaug auaacaauag aguuauucgg gugaaaggca agaacgcuau cggggugcgc      120

cucuuugacg aaggaaaagc cgcggcugau agacggagcu uccgcacgac cagacggcgc      180

cuguccagac ggcgcuggag acuuaagcug cuccgggaga uuuuugacgc cuauaucaca      240

cccgucgaug aagccuucuu uauccgccug aaagagucua aucuuagccc uaaggacucc      300

aagaaacagu acucuggcga uauucuguuc aacgacagaa gcgauaagga cuuuuaugaa      360

aaauacccga ccaucuauca ccuccggaau gcucugauga cagagcaucg caaguucgau      420

gugagagaga uuuaccuggc cauccaccau auuaugaaau uucgggggca cuuccucaac      480

gcuacccccg ccaauaacuu uaagguugga agacugaauc uugaagagaa guucgaggaa      540

cugaacgaca ucuaucaacg cguguuuccu gaugagucca uugaguuccg gacagacaau      600

cucgaacaga ucaaagaagu ccugcuugac aacaagagaa gccgcgcgga ucggcagaga      660

acccuggugu cugacaucua ccaguccagc gaggauaaag acauugaaaa gcgcaauaaa      720

gccguugcua cggagauccu caaggccucu cugggcaaca aagcuaagcu gaacgugauu      780

accaaugucg agguggauaa ggaagccgcg aaagaguggu ccaucacauu cgacagcgaa      840

ucuauugaug acgaucucgc caagaucgag ggccaaauga ccgacgaugg gcaugaaauc      900

auugaggugc ugcggucccu uuacagcgga aucacacugu ccgcuauugu ccccgagaac      960

cacacccucu cucagagcau gguugccaaa uaugaccugc acaaggacca ucuuaaacug     1020

uuuaagaagc ucaucaaugg caugacggau acaaagaagg cuaaaaaccu gagagccgcc     1080

uacgacgggu auauugaugg agugaagggc aaagugcugc cucaggaaga cuucuacaag     1140

cagguccaag ugaaucucga ugacuccgcu gaggcgaacg aaauccagac cuauaucgau     1200

caggacauuu uuaugcccaa gcagcgcaca aaagccaaug ggagcauccc ucaccaacug     1260

cagcagcaag agcuugauca gauuaucgaa aaccagaagg cuuacuaucc guggcuggcc     1320

gagcucaauc ccaacccuga caaaaagcgg cagcaacugg ccaaauacaa acuugaugag     1380

cugguuaccu ucagagugcc cuacuauguc ggaccuauga uuaccgcuaa ggaccagaaa     1440

aaucagucug gcgccgaauu ugcguggaug auccgcaagg agcccggcaa cauuacaccg     1500

uggaauuucg aucagaaagu ggaccggaug gcuacggcca accaauuuau caagagaaug     1560

accacaaccg acacauaccu ccugggggaa gauguucugc cugcccaguc ccuccuguau     1620

cagaaauucg aggugcuuaa ugagcugaac aagauccgga uugaccauaa acccaucagc     1680

auugaacaga agcaacagau cuuuaacgau cucuucaagc aguuuaaaaa ugucaccauu     1740

aagcaucugc aggacuaccu ggugucucaa ggacaguaua gcaaacgccc ucuuaucgag     1800

ggccucgcug augaaaagag auucaacucc ucucugagca cguacuccga ccugugcggg     1860

aucuuuggag ccaaacucgu cgaggaaaau gaucggcagg aggaccugga gaagauuauc     1920

gaauggagca caauuuucga ggauaagaaa aucuauagag cuaaacuuaa cgaccugacc     1980

uggcucaccg augaccaaaa agaaaagcug gccacaaaac gcuaccaggg cugggggcgg     2040

cugucuagaa aacuucucgu gggacugaaa aauuccgagc accgcaacau uauggauauc     2100

cuguggauca ccaaugaaaa cuucaugcag auucaggcgg agcccgacuu ugccaagcuc     2160

guuacagacg cuaauaaggg caugcuggag aagacggaua gccaagacgu gaucaacgau     2220

cuuuacaccu cuccccagaa uaagaaagcc auuagacaga uccugcucgu cgugcacgac     2280

auucagaacg cuaugcacgg ccaagccccu gcgaagaucc auguugaguu cgcccgcggg     2340

gaagaacgga auccgagacg guccgugcag cgccagagac aggucgaggc ugccuaugaa     2400

aaggugagca acgagcuggu guccgcuaaa guucggcaag aguuuaagga agccaucaau     2460

aacaaacgcg auuucaagga cagacuguuu cuuuacuuca ugcagggagg cauugauauc     2520

uauacaggga aacagcucaa cauugaccaa cugucuagcu accagaucga ucacauucug     2580

ccccaggccu uugucaagga cgauucccuc accaaucggg ugcugacaaa cgagaaucag     2640

gugaaagcug acucuguccc uaucgacauu uucggaaaga agaugcuuag cguguggggc     2700

cgcaugaaag aucaagggcu gaucuccaag ggcaaauaua gaaaccucac caugaauccc     2760

gaaaacauca gcgcgcauac ggagaaugga uuuauuaacc ggcagcuggu ugagaccaga     2820

caggugauca agcuggccgu caauauucuu gcugacgaau acggcgauuc uacacagauc     2880

auuuccguga aagccgaccu cagccaccaa augcgcgagg auuucgaacu gcugaagaac     2940

cgggacguua augauuauca ccaugccuuu gacgcuuacc ucgccgcguu caucgggaac     3000

uaccugcuua agagauaucc uaaacuggag ucuuacuuug uguauggaga uuucaagaaa     3060

uucacccaga aggaaacaaa aaugcgccgg uuuaauuuca ucuacgaccu caagcacugu     3120

gaucaggucg ugaacaagga gaccggcgag auucugugga cgaaagacga agauaucaag     3180

uauauuagac auuccuuugc uuacaaaaag auccugguua gccacgaggu gcgcgaaaaa     3240

cggggagccc uuuauaauca gacaauuuac aaggccaagg acgacaaagg gucuggccaa     3300

gggagcaaga aacugaucag aaucaaggau gacaaagaga ccaagauuua cggcggauau     3360

uccggcaaga gccucgcuua caugacaauc guccagauua ccaaaaagaa caaagugucu     3420

uaucgcguga ucgggauucc cacccuggcc cucgcgcggc ugaacaaacu ugaaaaugau     3480

uccacagaga acaauggaga gcuguacaag aucauuaagc cgcaguucac gcacuauaaa     3540

gucgacaaga agaacggcga aaucaucgag accacagaug acuuuaaaau uguugugagc     3600

aaggugagau uccagcaacu caucgaugac gcugggcagu uuuucaugcu ggccuccgau     3660

accuacaaaa auaacgccca gcaacugguc auuucuaaua acgcucuuaa ggccaucaau     3720

aacacaaaua uuaccgacug cccucgggau gaccuggaac gccucgauaa ccugagacuc     3780

gacagcgcuu uugacgagau cgugaaaaag auggauaagu auuucuccgc cuacgacgcg     3840

aauaacuuuc gggaaaagau ccgcaauucu aaccugauuu ucuaccagcu ucccguugag     3900

gaucaguggg agaauaacaa gaucacggaa cugggaaaaa gaacagugcu cacccggauu     3960

cugcagggcc ugcaugccaa cgcuaccaca accgacauga gcaucuucaa gauuaaaaca     4020

ccuuuugggc aacuuagaca gcgcuccggc aucucucuga gcgagaaugc ccagcucauc     4080

uaucaguccc ccacgggacu guucgaacgg agaguccaac ucaacaagau uaaguga        4137


<210>  4357
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4357
augaccaacg agaaguacag caucggccug gacauuggga cauccucuau cggauucgcc       60

guggucaaug auaacaauag aguuauucgg gugaaaggca agaacgcuau cggggugcgc      120

cucuuugacg aaggaaaagc cgcggcugau agacggagcu uccgcacgac cagacggcgc      180

cuguccagac ggcgcuggag acuuaagcug cuccgggaga uuuuugacgc cuauaucaca      240

cccgucgaug aagccuucuu uauccgccug aaagagucua aucuuagccc uaaggacucc      300

aagaaacagu acucuggcga uauucuguuc aacgacagaa gcgauaagga cuuuuaugaa      360

aaauacccga ccaucuauca ccuccggaau gcucugauga cagagcaucg caaguucgau      420

gugagagaga uuuaccuggc cauccaccau auuaugaaau uucgggggca cuuccucaac      480

gcuacccccg ccaauaacuu uaagguugga agacugaauc uugaagagaa guucgaggaa      540

cugaacgaca ucuaucaacg cguguuuccu gaugagucca uugaguuccg gacagacaau      600

cucgaacaga ucaaagaagu ccugcuugac aacaagagaa gccgcgcgga ucggcagaga      660

acccuggugu cugacaucua ccaguccagc gaggauaaag acauugaaaa gcgcaauaaa      720

gccguugcua cggagauccu caaggccucu cugggcaaca aagcuaagcu gaacgugauu      780

accaaugucg agguggauaa ggaagccgcg aaagaguggu ccaucacauu cgacagcgaa      840

ucuauugaug acgaucucgc caagaucgag ggccaaauga ccgacgaugg gcaugaaauc      900

auugaggugc ugcggucccu uuacagcgga aucacacugu ccgcuauugu ccccgagaac      960

cacacccucu cucagagcau gguugccaaa uaugaccugc acaaggacca ucuuaaacug     1020

uuuaagaagc ucaucaaugg caugacggau acaaagaagg cuaaaaaccu gagagccgcc     1080

uacgacgggu auauugaugg agugaagggc aaagugcugc cucaggaaga cuucuacaag     1140

cagguccaag ugaaucucga ugacuccgcu gaggcgaacg aaauccagac cuauaucgau     1200

caggacauuu uuaugcccaa gcagcgcaca aaagccaaug ggagcauccc ucaccaacug     1260

cagcagcaag agcuugauca gauuaucgaa aaccagaagg cuuacuaucc guggcuggcc     1320

gagcucaauc ccaacccuga caaaaagcgg cagcaacugg ccaaauacaa acuugaugag     1380

cugguuaccu ucagagugcc cuacuauguc ggaccuauga uuaccgcuaa ggaccagaaa     1440

aaucagucug gcgccgaauu ugcguggaug auccgcaagg agcccggcaa cauuacaccg     1500

uggaauuucg aucagaaagu ggaccggaug gcuacggcca accaauuuau caagagaaug     1560

accacaaccg acacauaccu ccugggggaa gauguucugc cugcccaguc ccuccuguau     1620

cagaaauucg aggugcuuaa ugagcugaac aagauccgga uugaccauaa acccaucagc     1680

auugaacaga agcaacagau cuuuaacgau cucuucaagc aguuuaaaaa ugucaccauu     1740

aagcaucugc aggacuaccu ggugucucaa ggacaguaua gcaaacgccc ucuuaucgag     1800

ggccucgcug augaaaagag auucaacucc ucucugagca cguacuccga ccugugcggg     1860

aucuuuggag ccaaacucgu cgaggaaaau gaucggcagg aggaccugga gaagauuauc     1920

gaauggagca caauuuucga ggauaagaaa aucuauagag cuaaacuuaa cgaccugacc     1980

uggcucaccg augaccaaaa agaaaagcug gccacaaaac gcuaccaggg cugggggcgg     2040

cugucuagaa aacuucucgu gggacugaaa aauuccgagc accgcaacau uauggauauc     2100

cuguggauca ccaaugaaaa cuucaugcag auucaggcgg agcccgacuu ugccaagcuc     2160

guuacagacg cuaauaaggg caugcuggag aagacggaua gccaagacgu gaucaacgau     2220

cuuuacaccu cuccccagaa uaagaaagcc auuagacaga uccugcucgu cgugcacgac     2280

auucagaacg cuaugcacgg ccaagccccu gcgaagaucc auguugaguu cgcccgcggg     2340

gaagaacgga auccgagacg guccgugcag cgccagagac aggucgaggc ugccuaugaa     2400

aaggugagca acgagcuggu guccgcuaaa guucggcaag aguuuaagga agccaucaau     2460

aacaaacgcg auuucaagga cagacuguuu cuuuacuuca ugcagggagg cauugauauc     2520

uauacaggga aacagcucaa cauugaccaa cugucuagcu accagaucga ucacauucug     2580

ccccaggccu uugucaagga cgauucccuc accaaucggg ugcugacaaa cgagaaucag     2640

gugaaagcug acucuguccc uaucgacauu uucggaaaga agaugcuuag cguguggggc     2700

cgcaugaaag aucaagggcu gaucuccaag ggcaaauaua gaaaccucac caugaauccc     2760

gaaaacauca gcgcgcauac ggagaaugga uuuauuaacc ggcagcuggu ugagaccaga     2820

caggugauca agcuggccgu caauauucuu gcugacgaau acggcgauuc uacacagauc     2880

auuuccguga aagccgaccu cagccaccaa augcgcgagg auuucgaacu gcugaagaac     2940

cgggacguua augauuauca ccaugccuuu gacgcuuacc ucgccgcguu caucgggaac     3000

uaccugcuua agagauaucc uaaacuggag ucuuacuuug uguauggaga uuucaagaaa     3060

uucacccaga aggaaacaaa aaugcgccgg uuuaauuuca ucuacgaccu caagcacugu     3120

gaucaggucg ugaacaagga gaccggcgag auucugugga cgaaagacga agauaucaag     3180

uauauuagac aucuguuugc uuacaaaaag auccuuguuu cccacgaggu gcgcgaaaaa     3240

cggggagccc uguauaauca gacaauuuac aaggccaagg acgacaaagg gagcggccaa     3300

gggucuaaga aacucaucag aaucaaggau gacaaagaga ccaagauuua cggcggauau     3360

agcggcaagu cccuggcuua caugacaauc guccagauua ccaaaaagaa caaagugagc     3420

uaucgcguga ucgggauucc cacccucgcc cuggcgcggc uuaacaagcu ggaaaaugau     3480

ucuacagaga acaauggaga gcucuacaaa aucauuaagc cgcaguucac gcacuauaaa     3540

gucgacaaga agaacggcga aaucaucgag accacagaug acuuuaaaau uguugugucc     3600

aaggugagau uccagcaacu gaucgaugac gcugggcagu uuuucaugcu ggccagcgau     3660

accuacaaaa auaacgccca gcaacuuguc auuuccaaua acgcucugaa ggccaucaau     3720

aacacaaaua uuaccgacug cccucgggau gaccucgaac gccuggauaa ccucagacug     3780

gacucugcuu uugacgagau cgugaaaaag auggauaagu auuucagcgc cuacgacgcg     3840

aauaacuuuc gggaaaagau ccgcaauucc aaccuuauuu ucuaccagcu gcccguugag     3900

gaucaguggg agaauaacaa gaucacggaa cucggaaaaa gaacagugcu gacccggauu     3960

cugcagggcc uucaugccaa cgcuaccaca accgacaugu cuaucuucaa gauuaaaaca     4020

ccuuuugggc aacugagaca gcgcagcggc aucucccucu cugagaaugc ccagcugauc     4080

uaucagagcc ccacgggacu cuucgaacgg agaguccaac ugaacaagau uaaguga        4137


<210>  4358
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4358
augaagcaga uccugggccu cgaccugggg accacaagca uuggaugggc ccuugugaga       60

gaggcugaaa acaaagauga gcaauccucu aucguccgga uuggcguucg cgugaauccc      120

cugacgagcg acgaacagaa gaacuucgag caggggaaau ccaucaccac aaaugccgau      180

agaacccuca agcggucuau gcgcagaaac cugcagcggu acaaacuucg cagagaccac      240

cugaaggauu gccuccaaaa gaauggaauu aucacagaca aagcggugcu gagcgaaaac      300

ggcaauuaua ccacauuuca gaccuacuau cugcgggcua aggccgucaa cgagaaaauc      360

ucccucgagg aguucgcccg cgugcugcuu augcugaaua agaaaagagg guacaagucu      420

agccggaaga ugaaauccgc ugaugaagga cagcucauug acggcaugag cguugccaag      480

cgccuguaua acgauaauau cacgccuggc caguacgugc uuucucugcu cgaagagggg      540

agaaaauaua uuccggacuu uuaccggucc gaucugcugg acgagcucga uaagaucugg      600

accuuccaac aguguuuuua ccccgaaauu cugacaccug agugccgcaa acuuaucgcu      660

ggaaagggca aacaggccac cgcgaagauu uuccugggga aguauaaaau cuacacagcc      720

gacaacaaga gcaaagacaa gagacuccag gcuuaucaau ggcgggucga agcccugucu      780

ggacagcuuc ccaucgaggu gguugcuuuu guggucugug aaauuaaugg cgccauccag      840

gcguccagcg gguaccuggg agccauuucu gauagaucca aagagcucua uuucgagggc      900

cugaccgugg gccaauaccu ggugaagcag cucgaaaaga acccugacac gagccugaaa      960

aauaaggucu uuuaucgcca ggauuaccuu aacgaguucg aaaagaucug ggagaagcag     1020

gcugaauacc auacagagcu gacaccccaa cucaaaaagg agguucggga cauuaucauc     1080

uuuuaucaga gaacccugaa gucccagaaa gggcuugugg gauucugcga auuugagucu     1140

cgccagauug aagugaugau cgauggcaag cugaaaaaga aaaccgucgg gugucgggug     1200

ugcccuaaga gcuccccgcu guuccaagag uacaagauuu ggcacaaccu caauaaccug     1260

aaaaucguug aaaaggagcu cggaaaaagc agauuccuga aucaggacga gaaggaaucu     1320

cuuuuucagg agcugaacau uaaagaaaag cucuccaaaa ccgauauccu gaagcugcuu     1380

ugucucaagg ccaaagaguu cgaccugaau uuugagaagg uggaaggcaa ccgcacacag     1440

gcugagcugu uccgggccua ucaagccauc auugaaauga gcgggaaugg agauuacgac     1500

uuuucuaaaa ugacgagcuc cgagauccag gauaccguca caggcguguu cagaggccuc     1560

ggguauaaca ccgacauucu gucuuuugac gcugaaaagg aggagaaaac auucgauaag     1620

cagcccaugu accuucugug gcaucuccug uauagcuacg aaggagacuc cagcgauacc     1680

ggcaauaagg cgcugaucac gaaaauuaag aaacuuugcg gguuugaguc ugaagaguac     1740

gcccgcaucc ucacagacgu uaccuuccag uccgaauaug gaaaccugag caccaaggcu     1800

auucggagaa uccugccuuu uaugaaaaag ggcucugagu acuccgaggc cugucgccac     1860

gccggguauc ggcauagcga aagaucccuc acaaaggagg aacuggauaa aaagguguac     1920

aaagaccacc uugagcugcu cccccgcaau ucucugcgga acccugucgu ggaaaagauc     1980

cugaaucaaa ugauucacgu cgugaacacc guuguggaua cauauggcaa acccgacgag     2040

aucagaauug agcuugcucg ggaacucaaa aagagcgcca aagagagaga ugaaaugacg     2100

gcguccauca auaagaccgc ugccgagcau aaaaaguacu gcgagguccu gcagaaagag     2160

uucggauuua cacacguguc ucgcaacgac auuauccggu auaagcugua ccucgaacug     2220

gaaaacaaug gcuacaaaac ccuuuauagc gauacauaca ucccgagaga gaagcuguuc     2280

uccaaggagu ucgacauuga acauaucauu ccucagagca aacucuuuga ugacucuuuc     2340

uccaacaaga cccuggagug ucgcagcguu aauaucgaaa aaucuaacga cacggccuau     2400

gauuacgugc ugaauaagua uggggagcaa ggcauucuug acuacaaaaa cagaaucgaa     2460

aaucucuaua aggauuccaa gaucagccgg accaaauacc agaagcugau gaugaaaaac     2520

ucugacauua gcggaaauuu uaucgagcgc gaacugagag auucccagua cauugcuaag     2580

aaagcccggg agauccucga ggaacugguc aaguucgugg ugcccacaac cgggagcguu     2640

acagcucgcc uuagagagga cuggcagcug gucgauguga ugcaagaacu caacuggaag     2700

aaauaugaca agcugggacu gaccgaugug cgguacgaca aagauggccg cgagauuaga     2760

uauaucaagg acuggucuaa acggaaugau caccgccacc augccaugga cgcgcuuacg     2820

aucgccuuua caaagagauc cauuauccag uaccugaaca aucucaacgc uaagagcgac     2880

aaauccgggu cuauuuaugc caucgaacag aaggagcugu uccgggauaa aagaggcaag     2940

cucaaauuua uuccucccau gcccugcaau aucuuccgcg cugaggccaa gcggcaccug     3000

gaaaacgucc uugugagcgu uaaggcgaaa aacaaggugg ucaccccuaa uauuaacaga     3060

uccaaaggaa agaauggcau caacaaaaag auccagcuga caccgcgcgg gcaacuccau     3120

aaugagacca uuuacggauc uaugaaacgg uaugaaacca agugggagaa gaucggcagc     3180

ggguuugacg agagacagau ugccaaagug gcuaacaaga aauuccgcga agcccugcug     3240

ucccggcuug cugaguuugg aaaugauccc cagaaggccu ucacaggcaa aaacucucug     3300

gaaaagaauc cucucuacgu ugacgagcug cacacgcaga aggugcccga aaaagucaag     3360

aucgugaccc ucgagaaccu guacacaauu agaaaagaga ucaccccuga ucuuaagauc     3420

gacaaaguug uggaugccgg gguccgccgg gugcugcaag aaagacucga cgaguuugau     3480

gacgauagca agaaggcuuu cuccaaucug gacgaaaacc ccauuuggcu gaauggcaaa     3540

aagaugaaac gggugacaau caugggaguc agcaacgcgg uuccgcuuca ugacaagaaa     3600

gauuauaagg gcaaucuggu guucgacucu aaggggcgca aacagccugu ggauuuuguc     3660

aacaccggau ccaaccacca cguggccauu uacagagacg agagcggcga acuccaggau     3720

aucguugugu cuuucuauga ggcuacgaca cgguccaccc uggggauucc cgucaucgac     3780

aaggaguaca aaaaggccga aggauggaau uuucucuuca gcaugaaaca aaacgaguau     3840

uuuguguucc cuaaugaaga gaccggcuuu aaccccaagg aaaucgaucu gaugaauccu     3900

gacaacuaca caaccauuuc ucccaaucuu uaucgcguuc agaaauucuc caagugcaug     3960

uacgggaaca gcuccgugag agaguacguc uuucggcauc accuggagac aagcgugaau     4020

gauucuaagg gccugaaaaa caucgccuau aagaauguua aaagccucgu guaccuggaa     4080

ggaauuguca aggugagaau caaccauauu ggccagaucg uguccgucgg ggaguauuga     4140


<210>  4359
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4359
augagaaucg acaacggccu ggaggaacuc aaggugaaua accggauuua caauaucggg       60

cuggauauug gaaccagcuc cgucggcuuc gccguuacag acgcucagga uaaacuugug      120

cgcgugaagg ggaaaacggc caucggaguc agacuguuug aggaaggcca caccgcggcu      180

gagcggcgcg gguucagaac aacccggcgc agacucucuc ggcgcaagug gagaauuaac      240

cugcuuaauc aaaucuuuga acccuauauc agcgagauug acccuacauu cuuugcccgg      300

cugaaaaacu ccucucucgc cggaaaggau aagaaauucg agggcagccu gcuguuuccg      360

gaccgcaccg auuaccaguu cuauaaggac aaucccacaa uguaccaucu cagaaaacac      420

cugauggaaa aggaucggca guuugaccuu cgcgagaucu aucuggcuau gcaucacauu      480

aucaaauaca gaggcaacuu ccucaauacc acgccuauga accauuuuaa gaaugaagag      540

auugauuucu ccgcccuguu uucucggcuu aacgagcugu aucaggaaau cgacgaugag      600

aauauuuucg aacucaacau cgagaaugug gacgaaauca agaaaauucu gcugaaccac      660

gaccucuuca agauggagaa acaaaagcag guugcuaaac ugcuuaacgu ggauaccgac      720

gauaagcagc ugaaaaagga gaauacagcc cucgcgagca acuuuuccaa ggccauccug      780

ggguacaaau ucaaucuugc ucuggucacc aagauucccg accaggauaa aaaggaaugg      840

acaaucagcc ucggagacga ggauuuugac gauagacugg acaacgugcc uguugcccug      900

accgaaucuc aacucgaggu ggucgaagug cugcacucca uuuucaauag caucucucuu      960

aacgagaucg uggagaaugg cgaaacgcug uccagcgcua ugguccgcaa auacaacgau     1020

cauaagcacg accucaagcu gcuuaaaaau guuaugacaa cccuggagga ccggaagaaa     1080

gccaagaaaa uucaggggau cuauaacgug uuugugggaa aggauacaga ccucauuucu     1140

gauaaggaca aaaauaagua caaagauaag gacgaguucu auaaagaaau ccagaagaac     1200

cuggauaauu ccagcgacgc ggaagagauu aucaagagaa ucaaacagga ucaauuucug     1260

cccaagcagc gcaccuccaa aaacggcguc auuccucauc agcuccacca acgggagcug     1320

gaccuuauca uugaaaauca ggccaaguac uauccguucc ugaaagaggc uaaccccaac     1380

aagaaaagac ucaagcaggc caaguacaaa cuggaugaac ugaucgcuuu uaagauuccu     1440

uauuacaucg ggcccaccuc uggagccgag aauagcgacu ucuccuggcu uaaacgcaag     1500

aaagauggcc agauuacacc uuggaacuuu gacgagaagg uggaccggau cgaaagcgcc     1560

aauagauuca uccgccggau gacgaccaag gauacauacc uccuggcuga ggacguucug     1620

ccccucgaau cucugauuua ugagaaauac uccgugcuua acgaacugaa uaacgucaga     1680

aucaaucgcc aaaagcucag cgugaacgau aaacaggaca uuuauaauga gcuguuuaag     1740

aacucuaaaa ccguuacacu gaagcagcuu cagaaguacu ucgaggugac caaaaagcuc     1800

aaaacggucg uggucgaagg ccuggcggau ccgaagaaau uccugagcaa ucucgggaca     1860

uaucacgacu uuaagcugau cuucggagau auuguggacg auccugagua ccaaaccgac     1920

cuugaaaaca ucaucgagug guccaccauu uuugaagauc ggggcaucuu caauucuaag     1980

cugaacgaga uuacauggcu caccgacgag cagagaaaga agguucugag caaacgguau     2040

cagggguggg gacgcuuuuc caagaaacug cuugugggcc ucaagaauag ccaggggaac     2100

ucuauccugg augaacugug gaacacaaau cucaacuuca ugcaacugca gucccaggac     2160

gacuuugccg agcuuauuca ugcugaaaau gccaaacagu ucaaggccaa cgaucccgac     2220

aauccuugga acggaaucga ugcuauccug aaugacgccu acacgagccc ccaaaacaag     2280

aaagcgauua gacaggucgu gaagguugug caggauaucg agaaagcuau gggcaauaag     2340

ccccagaaaa uugccaucga auuuacccgg ucugccgaca agaacccuag acgcacagau     2400

ucccggauua agagacucag cgacaucuac aaagaggcug ccgagaaggu cgugggcgaa     2460

ucccugaaac uggagcuuaa ugaauaucuc accucuaaga aaaagaugac agauaagaug     2520

uaccuguauu ucacccaacu ggggacggac aucuacaccg gcaaaaagau ugauaucgac     2580

cagcucaaca auuaugacau ugaucacgug cugccgcaga gcuuucuuaa agacgauucc     2640

cuggacaacc gcguucucac aaauagaucu gauaacagcg agaaguccga caccgucccc     2700

cugcauaaau ucggagcuaa gauggggaac uuuuggcggc aacuggccga ugagggacuu     2760

aucacaaagc gcaaauacaa gaaucugacc acggacccug cgacaauuga uaaauauacc     2820

auccagggcu ucauuagacg gcagcucgug gaaacaagcc aggugaucaa gcugaccgcc     2880

aacauccucg ggggccugua ccccgaggaa accgagauuc uugaaauccc ugcuaaacug     2940

aaucaccaau uccgcgacau guuugaucuc gucaagguga gagagguuaa cgacuaccau     3000

cacgccuucg augcuuauau uacaguguuu gucggaaauu uccuguaccg gcgcuauccc     3060

aagcugagau cuuacuuugu guauggcgac uucaaaaagc ugaacaaaac ggacgaugac     3120

auccggaagu uuaauuuccu uaaccucuuu aauaaagaua agguugagga caacgaaacc     3180

gaugagauuc ugugguccaa agccgaaagc auccgcgaca ucaagaauau uuacaacuau     3240

aaguucaugc ucgugucuag agaggucuac acacggcacg gggcgauguu uaaucagacc     3300

aucuacccgg ccuccaaaaa cgauagcaag aaacugauuc cuaucaagaa aggaaaggac     3360

acagcuauuu auggcgggua cucuggaaau accgaugccu auauggcuau cguggccguu     3420

gaagacaaga aaggcgagaa guucaaagug gucggggugc ccacgagagc cgugagccgc     3480

cuugagcagc uggcugcgga ugaccaugau caguaccuca cagcccugca cgacguccug     3540

aagccuaacu ucaccaaaau caagaccaau cggaagacaa aagcuaagga agagguuacc     3600

acacccuuuc gcauucuugu gggcaaagug ggauauagac aacugaucca ggacggccag     3660

gucaagauga ugcucggguc cagcgccuac aaauauaacg ccaagcaacu ggugcucucu     3720

gaaaaguccc ugaaaguuau uaagaacaau aaaaaguaca acccugauga cgagaaucag     3780

gaucuuaucg acguguacga ugaaauucug ggagucguga accgggcuuu caaucuguau     3840

gacacgaacg gcuuucgcaa aaagcucaau gauaacagag uuaaauucgu ggagcugccc     3900

aucaauaaca uuuuugacgg gaccaaacuu guccagaugg gaaagcgggc cgugcugaca     3960

cagauccuca auggccugca ugcgaacgcu gcccucgggg aucugaaagc ccuuggcauc     4020

agcaccccgu ucggaaagau gcaauccaaa ucuggcauuc cucugagcau ggacgcuucc     4080

cugguuuacc agucucccac agggcucuuu gagagaaagc ugaaucuuag cgaucuguga     4140


<210>  4360
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4360
augaccaaga aaaacuacag caucggccug gacauuggga caaauuccgu gggaugggcc       60

gucaucacgg augacuauaa gguucccgcu aaaaagauga aagugcucgg caacaccgau      120

aagaaguaca uuaaaaagaa ucugcuuggg gcccuucucu ucgacucugg agagacagcg      180

gaagcuacca gacugaaacg gacagcccgc agacgguaua cccgcagaaa gaaccggcuu      240

cgcuaccugc aggagaucuu ugccgaugaa auggcuaaag uggacgagag cuucuuucaa      300

agacucgaug aauccuuccu gcggugggac gaugacaaua agaagcuggg ccgcuauccu      360

auuuuuggga acaaagccga ugucgugaag uaccaccagg aguucccgac aaucuaucau      420

cucagaaaac accuggcuga cucuagcgag aaggccgauc uucggcuggu uuaccucgcg      480

cuggcccaua ugaucaaauu ucgcggacac uuccuuauug aaggcgagcu gaaugcugaa      540

aacaccgacg ugcagaagau cuuuuccgac uucgucagag uguacgaucg gacguuugac      600

gauucucauc ucagcgagau uaccguugac gccgcuucca uccugacaga gaaaauuagc      660

aagucucgca gacuggaaaa ucucaucaag uauuacccca ccgagaaaaa gaacacacug      720

uucggcaauc uuauugcccu ggcgcucggg cugcagccua acuuuaaaau gaauuucaag      780

cuguccgaag augccaaacu gcaauucagc aaggacucuu auaacgagga ucucgaggaa      840

cugcugggaa agaucggcga cgauuacgcu gaccucuuua ccuccgccaa aaaucuguau      900

gaugcuaucc uucugagcgg gauucucacg guggacgaca acucuacaaa ggcuccccug      960

uccgccagca ugaucaaacg guacgccgag caccacgaag aucuugagaa gcugaaagaa     1020

uuuauuaagg cgaacaaguc cgagcucuau caugacaucu uuaaagauga aaccaagaau     1080

ggauacgccg gcuacauuga gaacgggguc aaacaggacg aguucuauaa guaccugaaa     1140

aauacacugu cuaagaucgc uggaagcgau uauuuucucg acaagaucga aagagaggau     1200

uuccugcgca aacagcggac cuuugacaac ggcuccauuc cucaccagau ccaucuucaa     1260

gaaaugcacg ccauucugag acgccagggc gauuacuauc ccuuccucaa ggagaaucag     1320

gaccggaucg aaaagauucu gaccuuuaga aucccuuacu auguggggcc gcuggcccgc     1380

aaggauagcc gguucucuug ggcugaguac cacuccgacg agaaaaucac acccuggaac     1440

uuugauaagg ugauugacaa agaaaagagc gccgagaagu ucaucacgag aaugacccuu     1500

aaugaccucu accugccuga agagaaaguc cugcccaagc auucucacgu uuaugagaca     1560

uacgcggugu auaacgaacu caccaaaauu aaguacguga augagcaggg aaaagauagc     1620

uuuuucgacu ccaacaugaa gcaagaaauc uucgaucaug ucuuuaagga gaaucgcaaa     1680

gugacaaagg aaaaacugcu uaacuaucug aauaaggagu ucccugagua ccggauuaaa     1740

gaccucaucg gccuggauaa ggaaaacaag ucuuuuaaug cuagccuggg gaccuaucac     1800

gaccuuaaaa agauucucga uaaagccuuc cuggacgaua agguuaacga ggaagugauc     1860

gaggacauca uuaaaacgcu gacacucuuu gaagauaagg acaugaucca ugagagacug     1920

cagaaguacu ccgauauuuu caccgccgac cagcuuaaaa agcuggagcg ccggcacuac     1980

accggauggg gcagacucag cuauaaacug aucaacggga uucggaauaa ggaaaacaau     2040

aaaacaaucc uggacuaccu uaucgaugac ggaucugcua accgcaauuu uaugcaacuc     2100

auuaacgaug acacccugcc cuucaagcag aucauucaga aaucccaggu cgugggcgau     2160

guugacgaua ucgaggccgu gguccacgac cugccgggca gcccugcuau uaagaagggg     2220

auccuccaau cugugaaaau cgucgaugaa cuggugaagg uuaugggaga caaucccgau     2280

aacauuguga ucgagauggc cagagaaaau cagacaacga accggggcag aucccagagc     2340

cagcaacgcc uuaaaaagcu gcaaaauucc cucaaagagc uggggucuaa cauucugaau     2400

gaggaaaagc cuagcuauau cgaggacaag gucgaaaacu cccaucuuca gaaugaccag     2460

cucuuucugu acuauauuca gaacggaaaa gauauguaca ccggcgacga gcuggauauc     2520

gaccaccucu cugauuauga caucgaucau auuauccccc aggcguucau uaaggacgau     2580

agcaucgaca accgggugcu gacauccagc gccaaaaaua gagggaaguc ugaugacguu     2640

cccucccuug auauugugcg cgcuagaaaa gccgaguggg uccggcugua caagagcggc     2700

cucaucucua agcgcaaauu ugacaaccug accaaggcug aaagaggagg ccugacagag     2760

gccgacaaag cgggguucau uaagcggcag cuuguggaaa cccgccagau cacgaaacac     2820

guggcccaaa uccuggaugc uagauuuaau accgaguccg acgaaaacga uaagguuauu     2880

cgggacguca aggugaucac acucaaaagc aaucuggugu cucaguuccg caaggauuuc     2940

gaguuuuaca aagucagaga gauuaacgac uaucaccaug cccacgaugc uuaccucaau     3000

gccgugguug gaaccgcccu gcuuaagaaa uauccuaagc uggcuagcga guucguguac     3060

ggcgaauaua agaaauacga cguccauaag cucaucgcga aauccagcga ugaccacucu     3120

gaaaugggga aggccacagc uaaauauuuc uucuacucca accugaugaa cuuuuucaag     3180

cgggugauuc gcuacagcaa uggaaaaguu aucgugagac cggucgugga guauuccaag     3240

gauaccgaag acaucgccug ggauaagaaa ucuaauuuuc ggacgauuug caagguucug     3300

agcuaccccc aggugaacau cgucaaaaag guggagacac aaaccggcgg guucuccaaa     3360

gagucuauuc uuccuaaggg cgacagcgac aagcugaucc ccagaaaaac aaagaaagcc     3420

uauugggaua ccaagaaaua cggaggcuuu gacuccccua ccguggcuua uucugucuuc     3480

guuguggccg auguggaaaa ggggaaggcg aaaaagcuca aaacagucaa ggagcuggug     3540

ggaauuagca ucauggaacg cuccuuuuuc gaggaaaauc ccguugaguu ccucgagaac     3600

aaaggcuacc auaauauccg ggaagacaag cugauuaaac uuccgaaaua uagccuguuu     3660

gaguucgaag ggggaaagag acgccuccug gcuucugccu ccgagcugca gaaaggcaac     3720

gagaugguga ucccugggca ccuugucaag cugcucuacc acgcccagcg gauuaacagc     3780

uuuaauucua cgaaauaccu ggauuaugug uccgcucaua agaaagaguu cgaaaagguu     3840

cucagcugug uggaagacuu ugccaaccug uacgucgaug uggagaagaa ucuuucuaaa     3900

aucagagcgg uugcugacuc cauggauaac uucagcauug aagagaucuc caauagcuuu     3960

auuaaccugc ugacccucac agcccugggc gcccccgcug acuucaauuu ucuuggagag     4020

aagaucccuc gcaaacggua uaccucuaca aaggaaugcc ugaacgccac ccucauccac     4080

cagagcauua cgggccugua cgagacaaga aucgaucucu ccaaaauugg ggaagaguga     4140


<210>  4361
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4361
augggcaagu acaaccuggg gcucgacauc ggaaccaaua gcgugggcug ggccgucguu       60

gaugagaaca aucagauugu gaaaaaggga gggaaagcuc uguggggcgu gagaauguuc      120

gacgaagccc aauccgcgaa ggagacacgg aucuaucgca acucuagacg gcgcauuguc      180

agacggaauc agcgccuucu gcuccugcag aaagaauuuu acgaugagau cauuaaggug      240

gaccagacgu ucuuucaaag acuuaaggau agcuucuuua aaaaggaaga caaacugaac      300

aagaauaacu auaccuucuu ugaugacgau aucacagacc gggaguacuu caaaaaguuu      360

cccaccaucu aucaccuccg cucccaucug cuguacaaug augagaagau ugacaucaga      420

augcucuauc uggcccuuca ccauauuguu aaauaccggg ggcacuuccu ggauucugga      480

gaagugaacg agagcaauuu uuccaagauc aaagacgucc ucgacacauu caacaauaag      540

augucugaaa ugagccugga guuugaggau aacgaagacu acuucugcaa aauugaguuc      600

gaugaaaauu uugagaacaa acuuaaggac aucaugcugu cccagcucag caagaaggaa      660

aaacgccagg agcugauuga ucuguucaag guggacaaaa agucucucgu uaacgagcug      720

cuuaucgaac ugcucgugaa uaaaaagguc aagcugaacu cccuuagcau caccaauggc      780

aaaaaguaug agguguguga aauugauuuu gagaaagagg aacuguucga gaagcucgaa      840

gacgcuaaaa agaucauuac agagcuguuu gauauccugg acgaaauugu gaacaucaag      900

aaaaucgucg auuacuauua cguucucaag auuaucggcg acaaugagua ucugucugaa      960

gccaugguga aaaaguacga caaacaugau aaggaccuua gagagcugaa guccuucauu     1020

aaaaccuauu gcccuaacaa guacuacgag uguuuucgga aaacgagcaa uccgaaccuc     1080

ugcaauuauc ccaaguacgu ggggaucaac ucugucaaua aaaagauuga acgcuucgcu     1140

cacuguaaaa aggaggaauu uuauaaguac cugaaauccc uuuucaacac caucaaggau     1200

gagaaugcca aacugaagau cgaccucuuu cagaaagaua uggagaacga cgauuuccug     1260

aauagacaaa acagcaacca caauuccucu auuccuaugc agcugaacuu uugcgaacuc     1320

aagaagaucc uggagaauca gagcaaauau uaccccuucc uuaugcugaa gaacuccgac     1380

ggauauagca caucugaucg gauuaucgcu auuuucaaau acaagauccc uuacuaugug     1440

ggcccccuca auaaaaacuc caaguacagc uggcugguua agaaaucuaa ugaaaacauu     1500

accccuugga auuaugagaa ggugaucgac cuggaugcca gcgcgaaaga auuuauccag     1560

agaaugcaaa acaaguguac auaccuuaaa ggggagaaug acuauugccu cccgaagaag     1620

uccauucugu ucucugagua caacugucug caguaucuca aucgccugau cauuaacgga     1680

gcccuuaucg aaaauaaacu gaagaacgag auuuacgaaa acaucuuucu cugcuacaaa     1740

aagcccacca aaaagagcau cgucgaguau cugaaaacga auuacaacua ugaugaaaca     1800

acccugaagu ccaauauucu ugaggugaac ugugacauga gcucuuacau ccaguucaag     1860

gagauuuaug gcgaagacuu ugagcaaaau cucaacaucg uugaagauau uaucaaagac     1920

aucacaauuu ucgaggauaa gaaaauccug gaaaagcggc ugagagagau uuaccaucuc     1980

uccgaggaca aagugcagaa gaucaagggg cugaauuaua aaggauacgc uacccuuagc     2040

aagcacuuuc ugaacaauuu cuacucuguc aacgaagaga ccggcgaaau uuauggcccu     2100

aucauccaga uuaugcgcga uacaaauuac aaccuccagc aagugaucua uaaugaccgg     2160

uacaacuuua ugaaagccau ugauaaguau aauaaaaaga accuggacac ggagaagaaa     2220

cugaacgucu ccgauuucau cgacgaguac auuuauguga gccccaucau gaagagaucc     2280

cuuauucagu cuuacaauau cauccaggaa cucgagaaaa uucuggggga aagcaucucc     2340

aaguacuaua uugagugcgc ucgcguucgg ucuaacagca aagaugugau caauuccaga     2400

uaccgccagc ugagcgaacu cuauaaggac ugcgagcauc aagucucuga uauuuccaag     2460

aacuacauca auaucaacaa acugaagaaa cagcuugagg acaauaagaa cgaacugcgg     2520

gccgacaaac ucuaucugua cuuuacccag uucggaaagu guauguauac acugaaggau     2580

auugaccuug augacguguu ucucggcaaa uacgauaucg accacauuua cccucagagc     2640

cugaucaagg augacucucu gaauaacaga guucucgcgu ccaaagagaa gaaugggaaa     2700

aaggauaacc aauucauuuu caauaucccc gaauuucugc cgaaaaacgc cgagcuuuuc     2760

uauaagaagc ugcucgaaug caaacugauc accaaggaga aauaccgccg gcugacaaug     2820

aagaaaauug acgaugacga gcuugacauc uuugugaaua gacagcuggu cgcuaccaac     2880

cagagcguga agggacucgu ggauguucug aagauuuaua aaggcgucaa gccuacgaau     2940

aucguguacu cuaaagccgu gaacauuagc gacuuccgga acaauuauaa ccucuacaag     3000

ucccgcacag cuaauaacua ucaccaugcc cacgaugccu accugaaugu cgugaucggg     3060

aaagcucuug acguuuacua ucaguguaac uacuuucaaa auuauaagga ugugauuaag     3120

cugaaagaca gaaacaucac caucaauccc gaaauuaucc ucagcaagaa ccgguacguc     3180

aauagaaacg gagagaaaaa ggugauuugg gaucuggaca auacccugaa acuuaucaag     3240

aacaaucugu auaaccgcuu cgauauuaca gaaaccauca ugacacggaa gucuaacaaa     3300

auguuuucca agagcuccau cucuccugcg aaaaauagca agaaccucau ucccaucaaa     3360

acguccgacc ccagaugcaa uguucugaag uacggcggca uuaaaucuaa cagcuauaau     3420

uucuacugua ucguggagcu cuacgauaag aaggggaaaa aguccuauau ucuggaagcc     3480

aucccuaaaa cccauucuga caaacuugag gaguacauca agaacagcau ugaugaaaag     3540

aaugucucca acuuuaagau cauuaauaaa aacaucaaga gcaauauuau cauugaguau     3600

aaaaagcugc gcuacgugau cacaggaaaa ucuggcaagu auuaccucau caagaacuuc     3660

aaagacagaa auuuuuccua uaacgcuauu aagaccauca aauacauuga aaaguacaau     3720

uaucagaaaa aguacgagau cccguucgag gaaacaaagg agaaaauuau ccuggccccc     3780

aagaaaaagg acaccaaaga aaucagccug acgcuugaua acugccaggc ccugcucgac     3840

gagauuaucu cucuguucaa gaaagauauu uauaacuacg acaucauuua uaagaucacc     3900

uccaagcucg aagagaauaa acaaaaccug acacuuacca aucugaucaa gguucugaca     3960

gagauucuca uccugcuuaa aaccaacgaa cgggggacgg cugaucugac acucauugac     4020

augagcaagg auaccggaac acuguguauc ucuaaaaccc ucaagccugg caugaaguuu     4080

auuuccgaga gccccaccgg guacuaucgc aaaaucauuu ucgaagugcc uaauggcauc     4140

uga                                                                   4143


<210>  4362
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4362
augaagagaa uccugggccu cgaccugggg accacaagca uuggaugggc ccuugugaac       60

gaggcugaaa auaacaauga ggccuccucu aucguccggc ugggcguucg cgugaacccc      120

cucacggugg augaaaaauc uaauuucgag aaggggaaag cgauuaccac aaacgcugac      180

agacagcugc ggcacggagc ccgcaucaau cuucaaagau acaagcugcg gcgccagaac      240

cuccaugauu gccugcagaa acagggcugg cuggggaccg aagccaugua ugaggaggga      300

aaggcuucca cauuugaaac cuacaagcuc agagccaaag cugccgagga agagauuucu      360

cugcacgagu ucgcgcgggu ccuuuuuaug cugaauaaga aacgcggcua uaagagcaac      420

agaaaagcca auaacaagga agacggccaa cucuucgaug ggaugacaau cgcuaagaaa      480

cuguacgagg aacaucuuac cccugccgag uauucccugc agcuccugaa uaagggaaag      540

aaguuuacgc agggcuacua ucggucugac cugaacgcug aacucgagcg caucugggau      600

gagcagaaaa aguacuaccc ggaaauucug accgacgagu ucaaacaaca gcuugaaggg      660

aagacaaaga ccaauacaag caaaaucuuu cuggccaagu auggaauuua cuccgcggau      720

cucaaaggcc uggacagaaa guuccagccc cuuaaauggc ggguggaggc ccugcagcaa      780

cagguugaua aggaagugcu cgcuuuuguc aucagcgacc ugaaggggca gauugccaac      840

accucuggac ugcucggcgc uaucuccgau cgcagcaaag agcuguauuu caacaagcaa      900

acggugggcc aguaccuuug ggccucucug gaggaaaauc cucacauuuc caucaaaaac      960

aagcccuuuu auagacagga cuaccucgac gaguucgaga aaaucuggga gacacaggcc     1020

gcuuuucaua agcaacugac cccugaacuu aagcaggaga uucgggauau cauuaucuuc     1080

uaucagagac cccugaaaag caagaaaucu cucauuuccg uuugugagcu ggaacagcgc     1140

aaggugaaag cgacaaucga cgggaaggag aaggaaauca ccauuggacc uaaagucgcc     1200

ccgaaaagcu cccccguguu ccaagaguuu cggaucuggc agaaucugaa caaugugcuc     1260

cugauugaua acgacaccaa ugagaaaaga ccucuugaug aagucgagcg caaccugcuc     1320

uacaaggaac ugucuaucaa agcuaagcug agcaaaacag aggcccuuaa gauucucaau     1380

aagaaaggca agcaguggga ccugaacuac cgggaacugg aggggaauag aacgcaggcc     1440

auccucuucg auugcuauaa ccgcaucauu acccugacag gacacgagga augugacuuu     1500

aaaaagauca aggcuuccga gauucggcac uacguuagca ccaucuucaa gaaucuuggc     1560

uuuucuacag aaauucugga uuucgacccc ucccucaaga aacaugagcu ggaaaagcaa     1620

ccuauguauc agcuguggca ccuucucuac agcuaugagu cugauaacag cagaaccggg     1680

aaugaguccc ugcugcgcaa acucgaaacg acauuuggau uccccgagga auacgccacc     1740

gugcugugcg acguggucuu ugaggaagac uauggcaacc uuucugugaa ggcgaugcgg     1800

gagauccugc cguaccucca ggcuggcaac gauuacagcc aggccugugc cuaugcuggg     1860

uacaaucauu ccagacacag ccugaccaaa gaggaacugg accaaaaggu uuauaaggag     1920

cgccuugaac uccugccuaa aaacucucug cggaaucccg uggucgagaa gauucucaac     1980

cagaugauca augugaucaa cgccauuauc gaugaguacg gaaaaccuga cgaaauuaga     2040

aucgagaugg cucgggaacu gaaguccagc gccgcggauc gcaaaaagac aacccacgcc     2100

auuucucagg gcaaugcuga gaaccaaaga auccgggaaa uccuugagaa ggaguucucc     2160

cugagcuaua uuuccagaaa ugacaucauu aaauacaagc ucuaugagga acuggaaccc     2220

aacuacuaca aaacacugua uucugauacg uacaucacca aggacaaacu uuuuagcaag     2280

gauuucgaca uugagcauau cauccccaaa gcccgccucu uugaugacuc cuucucuaau     2340

aagacacugg aagcucggaa cauuaaucug gaaaagagca acaaaaccgc cuucgauuuu     2400

aucaaggaga aauaugggga agacggagcg gaggccuaca agaaaaagcu cgacaugcug     2460

cuugaaaaug augcuauuuc cagaccuaag uauaacaacc ugcuccgcgc cgaggcugac     2520

aucccgagcg auuucauuaa uagagaccug cggaacacac aguacaucgc caaaaaggcc     2580

ugcgaaaucc ugggcgagcu uguuaaaacc gugacgccca ccacagggaa gauuaccaau     2640

cgccugagag aggauuggca gcucgucgac gugaugaaag aacugaacuu ugagaaguau     2700

gaaaagcucg gccugacaga gaucgucgag gaucgggacg gacgcaaaau uaagagaauc     2760

aaagauugga ccaagcggaa ugaccaccgc caucacgcua uggaugcgcu ugccauugcu     2820

uucacgaaac cuucuuuuau ccaguaccug aacaaucuca acgccagauc caauaagggc     2880

gacagcauuu acgccaucga aaacaaggag cugcauuaug aagaggggaa acugcgguuc     2940

aaugcuccca ucccugugaa cgaauuucgc gccgaggcga agagacaccu uucugcuauu     3000

cugguuucca ucaaagccaa gaauaaagug augacacaaa acgucaauaa gauuaagacc     3060

aaacacggaa ucauuaagaa aauccagcuc acaccccggg gcccgcugca uaacgagacc     3120

aucuacggga ccaagaugag accuauuauc aaaaugguga agguuggagc cgcucucgau     3180

gaagccacaa uuaacaaagu gagcucuccc gcgauccgcg aggcucugcu uaagcggcug     3240

aaugaauaua gcggcaacgc caagaaagcc uucacgggga agaauacccu cgagaaaaac     3300

ccuauuuacc ugaaugcugg cagaacaaag accguccccu cccuggugaa aacaguggaa     3360

ugggagagcu uucacccuac ccgcaaacuu aucgacaagg accugaacgu ugauaaaguc     3420

guggacaagg gaauccggga gauucucaaa gccagacugg aagaguucaa uggcgaugcu     3480

aagaaagccu uuucuaaccu cgaagagaau cccaucuauc uggacgaagc gaagaagauu     3540

gcccuuaaac gcguguccau cgaggggguc cugagcgcua uuccgcugca uacgcucaag     3600

aaccaggccg gaaaaccuau cacaggcaag gaugggaaac ccgugcuggg aaauuacguu     3660

cagaccucca acaaucacca cauugcuuuc uauuacgacg aggauggcaa ccuucaagac     3720

aaugccguga gcuuuuucga agccgcugag agaaagagcc aggggauccc cgucaucgau     3780

aaggacuaca accgggauaa aggcuggcgc uuccuguuua ccaugaagca gaaugaauau     3840

uucguguuuc cuaacgaggc gacaggauuc auuccguccg agguugaccu caccgacgaa     3900

gccaacuacg gcaucauuuc ucccaaucug uaucgggugc agaaagucag cagaaucgau     3960

aaggggacau ccgcuucucg ggacuacugg uuucgccauc accucgagac gauucugaac     4020

gaugacgcca aacuuaagaa ucuggccuuc aaaagaaucc ggggacugcu cgaacugaag     4080

gauaucauua aggugcgcau caacagcacc ggcaaaauug uugcuguggg ggaguaugac     4140

uga                                                                   4143


<210>  4363
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4363
augaagaaaa uccugggccu cgaccugggg accacaagca uuggaugggc ccuugugaac       60

gaggcugaaa cggaguccga aaagucuagc aucauuagac ugggcguccg gguuaauccc      120

cucaccaucg augagaaagg gaacuucgaa aagggaaaag ccauuacaac caauuccgac      180

cgccagcaaa gacacucugc gcggaucaac cugcagcgcu acaaacuuag acggcagaau      240

cugugcgauu gucuccagau cggcgggcug cugggaagcg aguccaugua ugaggaaggc      300

aaggagucua cauuugaaac cuacaagcuc cgcgcuaagg ccgccacaga gaaaguggcu      360

cugcaugagu ucgcccggau ucuuuuuaug cugaacaaga aaagaggcua uaagagcaau      420

cgcaaggcua acuccaaaga agacgggcaa gccuucgaug gaaugaccau cgcgaagaaa      480

cucuacgagg aaaaucugac gccugccgag uauagccuuc agcugcucaa caagggcaaa      540

aaguuuucuc cguccuacua uagaagcgac cuggaaucug agcugaaucg gauuugggag      600

gaacagaaaa aguacuaccc cgagauccuc accgaugacu ucuaucagca acuggaaggg      660

aagacaaaag ugaacaccac aaagauuuuu cuugcuaaau acggaaucua uaccgccgau      720

cugaagggcc ucgacaaaaa gaugcagccu cugaaguggc gcauugaggc ucuuaauaaa      780

caggucgaua aggaagugcu gacguucguu guguccgacc ucaaagggca gaucgagaag      840

acaagcggac ugcugggcga gaucucugau agauccaaag aacucuacuu uaacaagcaa      900

accgucggcc aguaugugug ggccagccug aaggagaauc cccacguuuc cauuaaaaac      960

aagccuuucu accggcagga cuaucuugac gaauuugaga aaaucuggga aacacaagcg     1020

gccuuccaua aggagcugac ccccgagcuc aaaaaggaaa uucgcgauau cauuaucuuu     1080

uaccagagac cucugaaguc uaaaaagggg cuuaucagca uuugcgagcu ggaacagaaa     1140

aaggagaaag ugaucauuga gggaaaggaa aaagagaucc ucauuggcuc caaggucgcu     1200

cccaagagcu cuccgaugua ccaggaguuc cggaucuggc aaaaccugaa uaacgugcug     1260

cucaucgaca ccgaaauuaa ugaauccaga ccucuggaug aggaggaaaa agcccuucug     1320

uauaacgagc ucagcgugcg cgcuaagcug ucugaaacag aggcccugaa aacgaucaau     1380

aagaaaggga agcacuggga ccuuaacuuc aaggaacucg agggaaaucg gacccaggcc     1440

guccuguuug aguguuacaa cagaauuauc acacagaccg gccaugaaga gugcaauuuc     1500

aaaaagauua gcgcugaaga uauccgccac uauguugcga caauuuuuaa aaaccugggg     1560

uucaauaccg acauccucga uuuuaacccc ucccuggaga agcacgaacu ugagaaacag     1620

ccuauguacc aacuguggca ucuccuguau ucuuacgaga gcgacaaguc ccggacggga     1680

aaugaaagcc ugcuuaacaa gcucgaggcc aauuucggcu uuccccagga auaugcuucu     1740

gugcuggccg augccacauu cgaggacgau uacggcaaca ucuccgugaa agcuauuaga     1800

aagauccugc cgcaccucca ugacggguac gacuauagcg aggccuguga acgcgcggga     1860

uacaaccacu cuguccgguc ccugaccaaa gaggaacuug cucagaaggu guauaaagau     1920

agacuggacc uccugccuaa gaauagccug cgcaaccccg uuguggagaa gauucuuaau     1980

cagaugauca acgucauuaa ugccaucauc guggaauacg gcaaaccuga ugagauucgg     2040

aucgagaugg ccagagaacu caaguccucu gcugccaaac gcgaggaacg gaccaagagc     2100

auuaaccaaa augcugccga gaacaaaaga aucacagaaa uucugcagac cgaguuuggg     2160

cuguccuaua ucucucggaa ugacaucguu aaguacaaac ucuaucagga gcuggaagcg     2220

aacggauuca agacacuuua cagcaauacg uacauuccca aggagaaacu guuuuccaag     2280

gauuucgaca ucgaacacau ugugcccaaa gcccgccucu uugaugacag cuucggcaac     2340

aagaccaucg aggcuagaga ugucaaucug gccaaaucua acaagacagc uuucgacuuu     2400

gugaaggaaa aauaugggga gcagggcauu gaggaauaca agaaaaagcu ggauacccuu     2460

cucaaagaga augccaucuc cagcggaaag uauaagaaac ugaugauguc ugaagcggac     2520

aucccuuccg auuucauuaa ccgggaccug agaaacacac aauacaucgc ccgcaaggcu     2580

uaugagauuc ucggcgagcu ggucaaaacc aucacgccga ccacagggaa gauuaccgac     2640

cggcuuagag aagauuggca gcugaucgac gugaugaaag agcucaauuu ugaaaaguac     2700

gaucgccugg gacugacaga gguuauugaa gacagagaug gccggaagau caaacgcauc     2760

aaggacugga ccaaaagaaa cgaucaucgg caccaugcca uggacgcucu uacgauugcc     2820

uucacaaagc ccagcuauau ccaguaccug aauaaccuca augcccgcuc ugauaaaagc     2880

ggguccauuu acgcuaucga gaccaaggag cugcacagag aaaacaauac acuccgguuu     2940

aacgcgccua ugccccugaa ugaguuccgc aaggaagcca aaagacaucu uagcgcuauu     3000

cuggugucua ucaaggccaa aaacaagguc augacccaaa augugaacaa aaucaagacc     3060

aaaaauggaa uuaucaagaa gguucagcuc acaccucggg gcgcccugca caacgagacg     3120

auuuauggga ccaaaaugcg ccccaucauu aagaugguga aaaucggcgc ugcccuggac     3180

gaagcgacaa ucaauaaggu cuccagcccg accauuagag aggcccuucu gaaacggcuc     3240

aacgaguacu ccggagaugc uaagaaggcc uuugcuggca aaaacgccau cacaaagaau     3300

ccuauuuauc ugaacgcgga acacaccaaa acggcucccg ccauggugaa gaucguggag     3360

ugggaaaugu acuauccuau uagaaaacuc aucacacccg accugaagau ugagaagguu     3420

aucgauaaug ggaucaaaga aauucuuaag aaacgccuga aggaguucgg aggcgacgcc     3480

aaaaaggcuu uuucuaaccu cgacgagugc ccuaucuacc uggaugaaga gaagaauauu     3540

acccugaaac gggucagcau cgaaggggug cuuuccgcca uucccgugca uacccugaag     3600

aaccacgcug gaaaagagau caaggacgag gauggcaaag ccguccucgg ggacuaugug     3660

cagacaucua auaaccauca cguugcgauc uaccaggaug ccaagggcaa ucugcaagac     3720

aacgugauua gcuucuuuga agcugccgag agaaaauccc agaauguccc gaucguggau     3780

aagaaccuca aucgcgacaa gggauggcac uuccuguaca ccaugaaaca gaacgaauau     3840

uuuguuuucc cuaaugagga aacaggcuuc gugcccucug agauugaucu uauggacgag     3900

aacaauuacg aaaugaucag ccccaaccug uuucgggucc agacgauguc cagaguggau     3960

uaugggaaga ccauuauccg cgaguacguu uuccggaacc aucuggaaac aauccucaau     4020

gacaaccuga aacuuaaggg acugguguuu aaaaagauua agagccucaa agcucuggag     4080

ggcaucguca aggugagagu gaaugccauu gggaaaaucg ucgccguugg agaauaugac     4140

uga                                                                   4143


<210>  4364
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4364
augaagaaaa aguacgccau cggcauugac aucgggacca acagcguggg augguccguc       60

guuacagaug acuauaaagu gcccucuaag aaaaugaagg uguucggcaa uacggagaag      120

agauacauua aaaagaaccu gcucgggacc cugcuuuuug augaaggaaa uacagcugag      180

aaccggcgcc ugaaaagaac cgcccggcgc agauauacac ggcgcagaaa ucggauccuc      240

uaccugcagg aaauuuucgc ggaggaaauc aacaagaucg acgauagcuu uuuccaacgc      300

cuugacgauu ccuuucugau ugucgaggac aaacagggcu cuaagcaccc uaucuucggg      360

acccuccagg aggaaaagga guaucauaaa caguuuccga caauuuacca ccugagaaag      420

caacuggcug auagcuccca gaaagccgac auccggcuca uuuaucuggc ccuugcucau      480

aucauuaagu accgcggaca cuuccuguuu gaaggcgauc ucaaaucuga gaauaaggac      540

gugcagcauc uguucaacga uuuuguugag auguucgaca aaaccgugga aggcagcuac      600

cuuuccgaga aucugcccaa cgucgccgac gugcucguug aaaaggugag caagucuaga      660

caccuggaga auauccugca cuauuuuccu aacgagaaaa agaauggacu cuucggaaac      720

uuccuggcuc uugcccuggg ccuccagccc aauuuuaaaa cgaacuucga acuggcggag      780

gaugccaaga uccaauuuuc caaagaaacc uacgaggaag accuugagga acugcucggg      840

aagauuggag augacuaugc ugaucuguuc aucgccacaa agagccugua cgacggcauu      900

cuccuggcug ggauccuuuc uaccacaaac uccaccacga aagccccucu gucuagcucc      960

auggucaauc gguaugagga gcaucagaag gaucucgcgc ugcuuaaaaa cuuuauucac     1020

cagaaucuga gcgacuccua caaggaagug uucaacgaua aacucaagga cggauaugcc     1080

ggcuacaucg agggcaagac aacccaggaa aauuuuuacc gcuucaucaa aaaggcuauu     1140

gagaaaaucg augggucuaa cuauuuuauu gaaaagaucg agagagagga cuuccugcgg     1200

aaacaaagaa cauuugacaa uggaagcauu ccccaucaga uccaucugcg cgaaaugcag     1260

gccaucauuc ggagacaagc uaaguucuac ccuuuucucg aggaaaacca ggauaagauc     1320

gagaaaauuc ugaccuuccg caucccguau uacgugggcc cccuugcccg ggggaagucc     1380

gaguucgccu ggcugaauag aaaaagcgac gaaaagauuc gcccuuggaa cuuugaugag     1440

auggucgaca aagaaaccuc ugcugagaau uucaucacac ggaugacgaa cuaugaucag     1500

uaccuccccg accagaaggu ucugccuaaa cacucccugc uuuaugaaaa guuugcggug     1560

uacaaugagc ucaccaaggu gagauacguc acagagcaag gaaagagcuu cuuuuucgau     1620

gccaacauga aacaggaaau uuuugaccgc accuucaaag uguaucggaa gguuacaaaa     1680

gagaagcuga uggauuuucu gucuaaggaa uuugacgagu uuagaaucgu ggaucuccug     1740

ggccuugaca aagauaauaa gagcuucaac gcuucccugg ggaccuacca cgaccucaaa     1800

aagaucgucu cugaagacuu ucuggauaac agcgagaaug aggacauucu ggaaaacgug     1860

guucuuacgc ucacacuguu cgaggaucgc gaaaugaucc ggaaaagacu ggagaaguau     1920

aaggacgugc ucaccgagga acagcggaaa aagcuggagc gcagacacua caccggaugg     1980

ggccggcuuu ccgccaaacu gauuaauggc aucagagaua aggucacacg caaaaccauu     2040

cucgacuauc ugaucgauga cgggacaagc aaccggaauu ucaugcagcu gaucaacgau     2100

gacacgcuuu cuuuugugga ugaaauuaga cucgcccaag gauccggcaa ggcugaggac     2160

uaccgcgccg aaguccagaa ucuggcgggg agccccgcua ucaagaaagg gauucugcag     2220

ucucucaaga ucguggauga gcugauugag guuaugggcu augacccgga acauaucgug     2280

gucgagaugg ccagagaaaa ccaguucacc aaucaagggc ggcgcaacuc ccagcagaga     2340

uacaaaaaga ucgagaaugc cauuaaaaac cuugacagca aucugaacuc caagauccuc     2400

aaagaauacc cuacaaauaa ccaggcucug caaaacgauc ggcuguuucu uuauuaccuc     2460

cagaauggca aggacaugua uaccgaugag gagcuggaca uugaucagcu gucucaauac     2520

gacaucgauc acauuauccc ccaggccuuc aucaaggacg auagccucga caacaaagug     2580

cugacaaagu ccgcuaaaaa ucgcggaaag ucugaugacg uuccuagccu ugaaauugug     2640

uauaaaaaga agaacuuuug gaaacagcug cucgacuccc agcugaucag ccaaagaaag     2700

uucgauaauc ugaccaaagc cgagcggggc gggcuuacga acgaagacaa ggcgcgcuuu     2760

auucagagac agcuggucga gacccggcag aucacaaaac auguggcccg cauucucgau     2820

accagauuca auacaaagcu ggacgaagcu ggaaaccgga ucagagaucc caaggugaau     2880

auuaugaccc ucaaaucuuc ccugguuagc caauuucgca aggacuacca gcuuuauaaa     2940

guccgggaga ucaacaauua ccaccacgcc caugaugcuu accugaacgc cgugguggcg     3000

acggcccucc ugaagaaaua uccccagcug gcuccugagu ucgucuacgg cgacuauccg     3060

aaguacaauu cuuauaaguc cagaaaaagc gccacagaaa aggugcuuuu cuacucuaac     3120

aucaugaauu ucuucaaacg cguucuggug uauagcaaga ccggggaggu ccggauuaga     3180

cccgugaucg aaguuaacaa agagacagga gaaauugugu gggauaagaa guccgacuuu     3240

cgcaccgucc ggaaagugcu cagcuacccu cagguuaacg uggucaagaa aaccgaggug     3300

caaaaucacg gccuggauag agggaagccc aaaggcuucu acaacgcuaa ucucucuccu     3360

cgccccaagg agggauccgu ggaaaaccug gucccggcca aacagagcuu ugacacaaag     3420

cgguauggcg gguacgccgg aaucuccaau ucuuaugcug uucuugugaa cggcauuauc     3480

gagaagggga aaaagaaaag caaggcggaa gugacggagu uccagggaau cuccauucug     3540

gccagaaaag auuuugacaa gaauccuaag caauaccucc ugaaccuggg cuauaaagac     3600

aucaagucua uuaucaaacu ucccaaguac agccuguucg agcucgaaaa ugggucccgg     3660

cgcaugcugg cuucuauucu cagcaccaac aauaaaagag gcgagaucca uaagggaaac     3720

gaaauguauc ugccugauaa guuugucaca cuucuguacc acgccaugcg ggugaaucgc     3780

acccuggagc ccggccacaa aaaguacguu gaagcccaua gacacguguu cgacgagcuc     3840

cugacauaua uccuugagua caacgagaaa auugucgggg cuaaggccaa uggaaaacgg     3900

aucguggaag cguauuccgc uagaaaggau accgacaacc uggaggaacu cugcaauagc     3960

uuuauuaacc ugcucaaccu gacggcccuu ggcucugccg uugauuucga auuucugggg     4020

acaaagaucc cucgcuaccg ggacuauacc cccuccagcc ugcucaaagc uacccugauu     4080

caucagucug ugacaggacu uuacgagacc agaaucgauc uguccaagcu cggcggggac     4140

uga                                                                   4143


<210>  4365
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4365
augaagaaaa uccugggccu cgaccugggg accacaagca uuggaugggc ccuuguguac       60

gaguccgaaa acgcugauga gaagucuagc aucgucagac ugggcguucg ggugaauccc      120

cucuccgugg acgaaaaagg gaacuucgag aagggaaaau cuauuacgac caaugccgau      180

cgccagcaaa cacacggcgc gagaaucaac cugcagcggu auaaacuucg cagagaaaau      240

cugaaggacu gccuccagaa acaggggugg auuggaaccg aggcucugua cgaggaaggc      300

aagacaagca ccuuugagac auauaugcug cgggccaaag ccgcugaaga ggagaucucc      360

cuccaugaac ugugucgcgu ccuuuucaug cugaacaaga aaagaggcua caagucuaau      420

cggaaggcca acaauaaaga ggaugggaag cucuuugacg gaaugaccgu ggcuaaaaag      480

cuguaugagg aacaccuuac gccugccgaa uacagccuga aacuccuguc cagcggcaag      540

aaauucaagc aagaguauua ccgcucugau cugaaggagg aauuugacag aaucuggaac      600

gagcagcaga aauacuaucc ggaaauucuc accgaugacu ucaagcagca acugcagggg      660

aaauccaagg gaaauacaag caaaaucuuu cuuggcaagu acgggauuua uucugcggau      720

cugaagggac ucgaccggaa acugcagccc cuuaaguggc gcguugaggc ccugaaacag      780

aagguggaua aagaaguccu cgcuuucgug aucuccgacc ugaacggcca aauugccagc      840

accucuggcc ugcucggggc uaucuccgac agaagcaagg agcuguacuu uaauaagcag      900

acaguuggac aguaucuuug gaccucccug aaagagucuc cucaugugag cauuaagaac      960

aaacccuucu accggcaaga uuaucucgac gaauuugaga agaucuggga aacgcaggcc     1020

acauaccaca aagagcugac cccugaacuu aagcaggaga uccgcgauau uaucauuuuc     1080

uaccagagac cccugaaguc caaaaagggc cucaucagcg ucugcgagcu ggaacaacgg     1140

aaagugaagg ugacaauuga cgggaaagag aaggaaaucu cuaucggacc uaaagucgcg     1200

ccgaagucca gccccauguu ucaggaguuc agaauuuggc agaaucugaa caacauccuc     1260

cugauugaua agggcaauaa cucuaaucgc ccucuuuaug aggaggaacg gaaccugcuc     1320

uacgaggaac ugagcguuaa agaaaagcug uccaaagccg aggcucuuaa ggugcucaau     1380

aaaaacggga agcaguggga ccugaauuau aaggagaucg aaggaaacag aacccaagcc     1440

gugcuguacg auuguuauaa ucgcauuauc ggcaugaccg gccaugagga augcaacuuc     1500

aaaaagcuca aggcuucuga cauccgguac aaugucaaga caauuuuuaa aaaccugggg     1560

uucagcacgg auauccuuga cuuugauucc agccugcagg gacacgagcu cgaaagacag     1620

cccucuuauc agcuguggca ucugcuuuac uccuacgaga gcgacaauuc ucgcaccggc     1680

aacgacgccc uccugcggaa gcuggagaca gccuucucca uuccuaagga auaugcugcg     1740

gugcucagcg auguuguguu ugaggacucc uacgggaauc ugucugucaa agccaugaga     1800

aacauccuuc cccaucugca agcuggacuc gauuauagcc aggccuguga aaaggccggc     1860

uacaaacacu cccgccgguc ucugaccaag aacgagcugg acaaaaagga auauaaggag     1920

aaacuugagc uccugccuaa gagcucccug cacaauccgg ugguugagaa aauucucaac     1980

cagaugauca augugauuaa cgcugucauc gaugaauacg ggaagcccga cgaaaucaga     2040

auugagcugg cccgcgagcu uaaaaucggc gcggcugaac gggagaauau gacaagaagc     2100

auuaaccagg gaaaugccga aaacaagcgg auccgcgaga uucuggaaaa agaguucgcc     2160

cucucuuaua ucuccagaaa ugauaucauu aaguacaagc uguacgagga acugaaaagc     2220

aacggcuuua agacccuuua uucugacacg uacaucucca aagauaaacu cuucagcaaa     2280

gacuuugaua uugagcacau cauuccuaag gcucggcugu ucgacgauuc ucugagcaau     2340

aagacaaucg aagccagaga cgugaacauc gcuaaaucca auaagaccgc cuuugacuuc     2400

auuagcgaga aauaugauca acaggggauc gaagcguaca agaaaaagcu cgacauucug     2460

cuucaggaug gaucuaucuc caaggccaaa uauaaccgcc ugcucaugaa ggaggcugac     2520

auucccagcg gcuuuaucga ucgggaccug agagauaccc aauacaucgc caaaaaggcu     2580

uaugagauuc uguccgaccu uguccgcaau gugacaccca ccacaggggc cguuacgucu     2640

agacugcggg aagauuggca gcucguggac gucaugaaag agcugaacuu cgaaaaguac     2700

cagaagcucg gacuggugga gguuaucgaa gaucgcgacg gcagaaaaau uaagcggauc     2760

aaagauugga ccaagcgcaa cgaccacaga caucacgcga uggacgcccu uacaauugcu     2820

uucaccaaac cuagcuacau ccaguaucug aauaaccuca augcccgguc cgauaagaac     2880

ucuagcauuu acgggaucga gaauaaggag cugcaucgcg aagacaacaa acugagauuu     2940

aaugcuccgc uucccaucaa cgaguuccgg gccgaagccc gcagacaccu gaaguccauu     3000

cucgugucug ucaaagcuaa gaauaaagug augacacaaa acgugaaugu uaucaagacc     3060

aaacauggcg ucauuaagaa ggugcagcug acgccucggg gagagcucca caacgcgacc     3120

uuuuauggca ccaaaaagag acccaucauu aaaaugguga aggucggggc cgcucuggau     3180

gaggugacaa ucaaaaaggu uagcuccagc uucauccgcg aagcccuucu ggaccggcuc     3240

cacgaguaca auggagaugc caagaaggcu uuuacaggca gaaacucuau ugacaagaau     3300

ccuaucuaua uugaugagga acauaaaaag accgugcccg cccuggucaa aacgaucgaa     3360

ugggagaugu accacccgac acgcaagaug auuuccccug accugaagau cgauaaagug     3420

aucgacaagg ggauucggga gauccuuaga acccgccugg cgaaauucaa cggagaugcu     3480

cagaaggccu uuagcaaccu cgacgaaaau cccauuuaua aagacgaggc caagaagauc     3540

acacugaaac ggguuucucu cgaaggcgug uccagcguca uuccucugca uaaacuuaag     3600

gauaugggcg ggaagcccgu gguuggaaaa gacggcaagc cugugugggg agauuacguc     3660

cagaccucua acaaucacca caucgcuuuc uacauggacg agaacgggaa ucugcaagau     3720

gacgugguga ccuuuuucaa agccgcgaga cgcaaguccc agggcguccc cguuauugau     3780

aagaacuaua aucaugacaa aggguggcac uuucuguuca caaugaagca gaacgaauac     3840

uuuguguucc cgaaugaggc uacgggauuc aucccuaccg agguggaucu cauggacgaa     3900

gccaacuaug auaugaucag ccccaaucug uaccgggucc agaaaauguc caagacaaaa     3960

caaggcauua ccgacaagag agauuauugg uuucggcauc accuugagac agugcugaac     4020

aaugaccuca agguucgcaa cagcaucuuc aaacagauua auucucugaa gccccucgaa     4080

gggaucguga aagucagaau uaacgccauc ggcaagaucg uggcuauugg agaguacgac     4140

uga                                                                   4143


<210>  4366
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4366
augaagagaa uccugggccu cgaccugggg accacaagca uuggaugggc ccuugugaac       60

gaggcugaau ccaauaacga ggccucuagc aucguccggc ugggcguucg cgugaauccc      120

cucacggugg augaaaaauc caacuucgag aaggggaaag cgauuaccac aaaugcugac      180

agacaacugc ggcacggagc ccgcaucaac cuucaaagau acaagcugcg gcgccagaau      240

cuccaugauu gccugcagaa acagggcugg cuggggaccg aagccaugua ugaggaggga      300

aaggcuucua cauuugaaac cuacaagcuc agagccaaag cugccgagga agagauuagc      360

cugcacgagu ucgcgcgggu ccuuuuuaug cugaacaaga aacgcggcua uaaguccaau      420

agaaaagcca acaauaagga agacggccaa cucuucgaug ggaugacaau cgcuaagaaa      480

cuguacgagg aacaucuuac cccugccgag uauucucugc agcuccugaa caagggaaag      540

aaguuuacgc agggcuacua ucggagcgac cugaaugcug aacucgagcg caucugggau      600

gagcagaaaa aguacuaccc ggaaauucug accgacgagu ucaaacaaca gcuugaaggg      660

aagacaaaga ccaacacauc caaaaucuuu cuggccaagu auggaauuua cagcgcggau      720

cucaaaggcc uggacagaaa acuucagccc cugaaauggc ggguggaggc ccucaagcag      780

caaguugaua aggaagugcu ggcuuucguc aucucugacc ugaaagggca gauugccaau      840

accuccggac uccugggcgc uaucagcgau cgcucuaagg agcuuuauuu uaacaaacag      900

acggugggcc aauaccugug ggccucccuc gaggaaaacc cucacauuag caucaagaau      960

aaacccuucu auagacagga cuaccuggac gaguuugaaa agaucuggga gacacaggcc     1020

accuuccaua agcagcuuac accugaacug aaacaagaga uucgggauau cauuaucuuu     1080

uaucagagac cccucaaguc uaaaaagucc cugauuagcg uuugugagcu ggaacagcgc     1140

aaagugaagg cuaccaucga cgggaaggag aaagaaauca ccauuggacc uaaggucgcg     1200

ccgaaauccu cucccguguu ccaggaguuc cggaucuggc aaaaccucaa uaacgugcug     1260

cuuauugaua augacacaaa cgagaagaga ccucuggaug aagacgagcg cuaccuccug     1320

uacaaagaac ugagcaucaa ggccaaacuu uccaagacgg aggcucucaa gauucugaau     1380

aaaaagggca aacaguggga ucugaacuau aaggaacucg aggggaaucg gacccaggcc     1440

auccuguuug acugcuacaa cagaaucauu acacuuaccg gacacgagga augugauuuc     1500

aaaaagauca aggccagcga gauucgccac uaugucucua caaucuuuaa aaaucugggc     1560

uucuccaccg aaauucucga cuuugauccc agccuggaga agcaugaacu ggagaaacag     1620

ccuauguacc aacuuuggca ccuccuguau ucuuacgaga gcgacaacuc ccggacgggg     1680

aaugaaucuc ugcucagaaa gcuggagaca accuucggau uucccgaaga guaugcuacc     1740

guucuuugcg acgugguguu cgaagaggau uacggcaacc ugagcgucaa agccaugcgc     1800

aagauccucc cguaccugca ggcgggcaau gacuauuccc aggcuugugc cuacgccggg     1860

uauaaccaua gccggcacuc ucugacaaag gaggaacuug aucagaaagu guacaaggag     1920

agacucgaac ugcugccuaa aaacucccuc cgcaaucccg uuguggagaa gauucugaac     1980

caaaugauca augucaucaa cgcuauuauc gacgaguaug gaaaaccuga ugaaauucgg     2040

aucgagaugg ccagagaacu uaagagcucu gcugccgacc ggaagaaaac cacacacgcg     2100

auuaaucagg gcaacgauga gaaucagugc auccgcgaaa uccuggagaa ggaguuugcc     2160

cucuccuaca uuagcagaaa cgacaucauu aaauauaagc uguacgagga acugaaaccc     2220

aauggguuca agacgcuuua cuccgauacc uauaucccca aagacaagcu cuuuucuaag     2280

gauuucgaca uugaacauau caucccuaaa gcucggcugu uugaugacag cuacuccaac     2340

aagacacugg aggccagaga cauuaaucuc gcuaaaucua acaagaccgc cuucgauuuc     2400

gugaaagaaa aguauggaga gcaaggcgcg gaggccuaca agaaaaagcu ggacaugcuu     2460

cuggggaaua acguuaucag caaaccgaag uauaauaaac uccugaugac agaagcugau     2520

auucccuccg acuuuaucaa ccgcgaucug cggaacaccc aguacauugc cagaaaggcu     2580

ugugagaucc uuggcgaacu ggugcgcacg gucaccccua caaccggaaa gaucacaaau     2640

agacuccggg aggacuggca gcugguggau gucaugaaag aacucaacuu cgagaaguau     2700

gagaaacugg gccuuaccga aauuguggag gaccgcgaug ggagaaagau caaacggauu     2760

aaggacugga cgaagcgcaa ugaucacaga caucacgcca uggacgcccu ggcuaucgcg     2820

uuuacaaaac ccagcuacau ucaguaccuc aacaaucuga acgcccgguc uaauaaggga     2880

gauuccaucu augcuauuga aaacaaagag cugcauuacg aggaaggcaa acuucgcuuc     2940

aaugccccua uccccguuaa cguguuuaga gccgaggcua aacggcaccu gagcgccauc     3000

cucgucucua uuaaggcgaa gaauaaagug augacccaaa acguuaauaa gaucaaaaca     3060

aagcacggga uuaucaaaaa gauucagcug accccgcgcg gaccucucca uaacgaaacc     3120

aucuauggca caaaaaugag acccaucauu aagaagguga aagucggggc ugcccuggac     3180

gaggccacga ucaauaaggu guccagcccu gcuauucggg aagugcuucu gaaaagacuc     3240

aacgaguacu cuggcaacgc caagaaagcg uucaccggaa agaauacacu ggacaagaac     3300

cccaucuauc ugaaugcuga gcgcaccaaa acaguuccug ccagcgucaa gaccguggaa     3360

ugggagaacu accaccccau ucggaaaccg aucacgccug aucuuaaggu ggacaaaguc     3420

aucgauaagg gcauugggga aauccugaag gccagacucg aggaauuuaa uggagacgcu     3480

aaaaaggccu ucuccaaccu ggaggagaau cccauuuauc ucgaucagac aaagaagauc     3540

gcucugaaac gcgugagcau ugaaggcguu cuuucuacca ucccccugca uacccugaag     3600

aaccaggccg ggaagccuau uaugggaaaa gacggcaagc cggugcucgg gaauuacguc     3660

caaacaucca acaaucacca caucgcguuu uacuaugaug aggacggcaa ccugcaggau     3720

aaugccguga gcuucuuuga agcugccgag cggaaauccc agggaaucuc uguuauugac     3780

aaggauuaca acagagacaa aggcuggcgc uuccuuuuua ccaugaagca gaaugaauau     3840

uucguguucc ccaacgaggc uacaggguuu aucccuagcg aggucgaccu gacggaugaa     3900

gccaauuacg gaauuaucuc ccccaaccuc uaucgggugc agaaaauguc uagaauugag     3960

aagggcacca gcguuucccg cgacuacugg uuccggcauc accuggaaac aauccucaac     4020

gaugacucua gacugaagaa uagcgccuuu aaacggauca acucccuuaa gccucuggag     4080

gggauuauca aagugcgcau uaauagcauc ggaaagauug ucgcuguggg cgaguaugau     4140

uga                                                                   4143


<210>  4367
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4367
augaagaaag gcuacagcau cgggcuggac auuggaacca acuccguggg cuucgccguc       60

aucacagaug acuauaaggu ucccucuaaa aagaugaaag ugcucgggaa uacggauaag      120

agauuuauua agaaaaaccu gaucggagcc cuucuguucg acgagggcac cacagccgaa      180

gcgcggcgcc ucaagagaac cgcucggcgc agauacacac ggcgcaaaaa uagacugcgg      240

uaucuucagg agauuuuuag cgaagagaug uccaaggugg auucuagcuu cuuucaccgc      300

cuggacgauu ccuuccucau cccugaagac aaaagagagu cuaaguaccc gaucuuugcc      360

acccuggagg aagagaagga auaucauaaa aaguucccca caauuuacca ucugcggaaa      420

caccucgccg auagcaagga gaaagcugac cugcgccuua ucuaucuggc ccucgcucac      480

augauuaagu acagagggca uuuucuguac gaggauaccu ucgacaucaa aaacaaugau      540

auucaaaaga ucuuuaacga guucauuucc aucuaugaca augccuuuga aggaagcucu      600

cuuuccggcc agaacgcgca ggucgaagcc aucuucacgg acaagauuag caaaucugcu      660

aagcgggagc gcgugcugaa acucuucccu gaugaaaagu ccaccggccu guuuagcgag      720

uuccugaaac ucaucguugg gaaucaggcc gacuuuaaga agcacuucga ucuggaggac      780

aaagcucccc uucaauuuuc uaaggauaca uacgacgaag aucuggagaa ccuccuggga      840

cagauuggcg acgauuucgc cgaccuuuuu guggcggcca aaaagcugua ugaugcuauc      900

cuccuguccg ggauucugac cgucacagac ccuagcacca aagcuccccu cuccgcuucu      960

augaucgaaa gauacgagaa ucaccagaac gaccuggugg uucuuaagca guucaucaag     1020

aauaaccugc cugaaaauua uguggagguc uuuagcgaug acuccaaaaa cggauacgcc     1080

ggcuaugugg ggguggauag caagauucgg aaaagaaacg ccaagcucgc uacggaggaa     1140

gaguucuaca aauaccugaa gaauauccuu aacaauguca agggagcgga cuauuuucug     1200

gauaaaauug aacgcgagga cuuccuccgg aagcaaagaa cauuugauaa cggcucuauc     1260

ccgcaucaga uucaucugca ggaaaugaau gccauccugc gccggcaggg cgagcacuac     1320

cccuuccuca aagagaacag agaaaagauc gagaaaauuc ugaccuuccg caucccuuau     1380

uacguugggc cccuugcucg gggaaauaga gacuuugccu ggcugacacg caacuccgau     1440

caagccauuc ggccuuggaa uuucgaagag aucguggaca aggcuagcuc ugccgaggau     1500

uuuauuaaca aaaugaccaa uuaugaccuc uaccugcccg aagagaaggu gcugccgaag     1560

cacagccuuc ucuaugaaaa auucgcgguc uacaacgagc ugaccaaggu gaaauuuauc     1620

gcugaaggcc ugagagauua ccaguuccuc gacuccgggc agaagaaaca aauuguuaau     1680

cagcuguuua aggagaagcg caaagugaca gagaaggaca ucauccacua ucuucauacg     1740

gucgauggau acgacggcau ugaacugaaa gggaucgaga agcaguucaa cgccucucuc     1800

agcaccuauc acgaucugcu gaaaauuauc aaggacaagg aauuuaugga ugacccuaaa     1860

aaugaggaaa uucuugagaa caucgugcau acacucacca ucuucgagga ucgggaaaug     1920

auuaagcaga gacuggccca auacgacucc cuguuugaug agaaaguuau caagagccuc     1980

acacggcgcc acuauaccgg auggggcaaa cugucugcua ugcuuauuaa cggcaucaga     2040

gacaagcaga cggggaagac aauucuggau uaccucaucg acgaugccua uuacaaucgg     2100

aacuucaugc agcugaucaa ugacgacgga cuguccuuua aagaaauuau ccagaaggcu     2160

caaguggucg gcaaaaccga ugacgugaag caggucgugc aggagcuucc cgggagcccu     2220

gccauuaaaa agggaauccu ccagucuauu aaaaucguug augaacuggu gaagaucaug     2280

agacauaacc ccgaguccau ugucaucgag auggcgcgcg aaaaucaaac cacagcccgg     2340

ggcaagaaaa acagccagca gagauacaag cgcauugagg acgcucugaa aaaucucgcc     2400

cccgggcugg auuccaacau ccuuaaggaa cacccuaccg acaaugugca gcugcaaaac     2460

gauagacucu uccuguauua ccugcagaau ggcaaagaca uguauacagg agaggcucuu     2520

gauauuaacc agcucucuag cuacgacauc gaucacauua ucccgcaagc cuucaucaag     2580

gacgacuccu uugauaaucg gguucugacg ucuagcaagg acaaccgcgg caaauccgau     2640

aaugugccca gcauugaggu cgugcagaag agaaaagcgu ucuggcagca gcugcucgac     2700

ucuaagcuga ucuccgaacg gaaauuuaac aaccuuacca aggccgagcg cgggggacug     2760

gaugaaagag acaagguggg cuucauuaaa cggcaacucg uugagacacg ccagaucacc     2820

aagcaugucg cucagauucu ggaugccaga uauaauacag aagugaacga gaaagacaag     2880

aaaaaucgga ccgugaagau caucacgcuu aagagcaacc uggucucuaa uuuucgcaaa     2940

gaguucagac uguacaaggu gcgggaaauu aacgauuauc accaugcuca cgacgccuac     3000

cucaaugccg uuguggcuaa agcgauccug aagaaauacc cuaagcucga gcccgaauuu     3060

gucuaugggg aguaccagaa guaugaucug aaaagauaca uuucccgcag caaggacccu     3120

aaagaagugg agaaggccac cgagaaauau uucuuuuacu cuaaccuucu gaauuucuuu     3180

aaggaagagg uucacuaugc ugauggaaca aucgugaaac gggaaaacau ugaguacagc     3240

aaggacaccg gcgaaaucgc cuggaauaag gagaaagacu ucgccaucau uaagaaaguc     3300

cucucccugc cccaagugaa caucguuaag aaaagagagg ugcagacagg gggcuucagc     3360

aaggaaucua uucugccgaa gggaaauucc gauaaacuua ucccucgcaa gaccaaagac     3420

auucuguggg auacgacaaa guacggcggg uuugacagcc ccgucaucgc uuauuccauu     3480

cuccugaucg ccgauaucga gaaaggaaag gcgaagaaac ucaagaccgu gaaaacacug     3540

gugggcauua ccaucaugga aaaggcugcc uucgaggaca acccuauuac cuuucuugag     3600

aauaaagggu accauaacgu ccggaaggaa aacauccugu gccuccccaa guauucucug     3660

uucgagcugg aaaauggaag acgccggcuu cuggccagcg cuaaagagcu ccagaagggc     3720

aacgaaauug uucugccugu guaccucaca acgcugcuuu aucacuccaa aaaugugcau     3780

aagcuggaug agcccgggca ccuggaguac auccagaaac auagaaacga auuuaaggac     3840

cuccugaauc uugucucuga guucagccaa aaauaugugc uggccgaugc gaaccucgaa     3900

aagaucaagu cccuguacgc ugacaaugag caggccgaua uugaaauccu cgccaacucu     3960

uuuauuaauc ugcuuaccuu cacagcucug ggcgccccgg cugccuuuaa auucuuugga     4020

aaggacaucg aucgcaaacg guacaccaca guuagcgaga uucugaacgc gacccucauc     4080

caccagucca ucacgggccu guaugagaca agaauugacc uuagcaagcu gggggaagac     4140

uga                                                                   4143


<210>  4368
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4368
augaaccuga gacacguggg caauuacaac aucgggcucg acaugggaac cgccagcguc       60

ggcugggcug ccauugauga gcagggggaa cugcuucauu ucaagggaaa acccacaugg      120

ggcucccggc uguuugacuc ugcgaauacg gcugccaccg cccgcaugcc uagagggcaa      180

cggcgcagau auauccggcg cagauggcgg cucgaucugc uucagaaguu cuuuagcgag      240

gaaauggcug agaaagaccc ggaguucuuu auucgccuga gacaguccuc ucucuggccc      300

gaagaucggg agggaggcca cacagccuac cauuggccuc uguucaacga cagcgauuuu      360

aaugaaaagg aguauuacga aaaauucccc accaucuuuc aucugcgcca guggcucaug      420

gagacagacg agaaggcuga uauuagacug aucuaucuug ccuuccacaa caucguuaag      480

caccggggca auuuucugca agaaaacaau gacucccucu cugcgcagaa cgccaauaug      540

cgcgagagcc uggaauccuu cugcauugcu cuugaggaau ggugugauga gaaagagcug      600

accugcagcu gugacccuca gaagcucgaa caccuguuua aagaggugaa guacagaucu      660

gauauccggg acggguucgc cgcucugcuc ggacuggccu gcuccggcga aaacgaggug      720

gacgucaaag cgauggccaa ggaaaugggg aaggcuauug ugggauauaa agccgauuuc      780

agcaagaucu uuacagagug ugacaaagag ggcagcuccu ucagacuuag cgaugacgaa      840

gggcccgagg cuuuucugga uauuugcccu gacgauggaa ucgcccucuu cgaagccauu      900

cagaugguuu acucuuccuu uauccugacc ggcaugcuua cggagaaggg ccugagccuc      960

uccaaaaucu cugcuuacaa ucaauauggg caggaccugg aaagccugaa ggcgcucgug     1020

aaagaguacg ucccgaacga guauuccagc uucuuucgcg gacccaccua cucuggcucc     1080

acagaauaug auccuagcaa ggcccagggg uacacccggu auaaucuggg acccucuaag     1140

aaaggcagcg gggcuaagcc uauggccuac gaggacuuca aaaaguccgu ggaacagcuu     1200

uuugccaaca caaacgcuau uaaugaugag agauacaaag agaugauggg acgcuucgcc     1260

gaagagaagu uucugcggag acuccgcacc ucugacaacg gccggauccc cuuccaacug     1320

aaucuugaag agaugacggc gauuaucauu aaccagggca aguauuaccc guuucugcuc     1380

gaacagcaag agaaacugga gagccugguu agauuccgca ucccuuauua cguggggccc     1440

cucacacgga ucaaugcuag acuggacucc aagggaaacg ccuguuucgc cuggagcaaa     1500

cgcaaggaug gcauggacuc ugcuuccauu aaaccuugga auugggauga agucaucgac     1560

aagcaugcca gcgcucaggc cuuuauugag cggaugaccg ggacaugcac cuaucuuaga     1620

ggagaacccg ugcugccccg guguucucuc cuguacgagg aauauugcgu gcugaacgag     1680

cuuaauggcg cgcgcuggac ccaggauggg gacaaguuca ucagauuuga uuacaaagac     1740

cgguccgaua uuaucgagga acucuucaag aaaagacgcg gccggaucca guacgccgcu     1800

gucgccgacu ggaugaagca agagagaagc cacgcuaacg uucauguguc cggaggccag     1860

ggggaaaaug ccuuugaguc ucgccugagc uccuaucacu ucuuuuguaa cgauguguuc     1920

ggagucgacg aacugccugg cucuuacgug gauaugguug aggagauuau ccucuggagc     1980

acacuguuug aagacagauc cauucuucgg gaggaaaucc agcgcaaaua uggggacgug     2040

cugaaugagg agcagauuaa gacgaucugc aagaaaagau ucagcggaug gggccggcuc     2100

ucucgcaagc ugcugugcuc ccuuaaagcg caaaccgaua acgggccgaa gucuauuaug     2160

gacauccucc aggaaggcga ucagaauaac ggaagagucg gccgggccau gguguuucag     2220

gagauccugc acgacgaaca acugggguuc gagcagaaaa uugaagagga gaacacacag     2280

ucuaugcucg aaggagagaa ucugcuuauc gaagaucugc ccggcucccc ugcuauucgc     2340

agaagcauca accaggccgu ucggauugug gacgagaucg ucgggaucgc uggacaugcc     2400

cccgauaaua uuuuuaucga agugacccgc gaggacgaua caaagaaaaa gggcaagaga     2460

accacgacac gguacucuaa cauucaaaaa gcccucgagg cucugaagga agagggggcg     2520

gaagcccuga gagagcuuaa aaccuauaag cacaaugaac ucgacaaacg ccugagccug     2580

uacuucaugc agaacggcaa gucccucuau agcggaaucc cucuggauau ucggagacuu     2640

ucugaguacc agaucgacca uaucauuccc caauccuaca ucaaggauga cagcuaugag     2700

aauauggcuc ugguccuccc gggcgaaaac caguccaaag ccgacaaucu gcugauuucu     2760

caggauaucc gcauuaagau gaaagccacc uggacagccc uucacaaggc cgggcucaug     2820

ggagacaaaa aguacaacaa ucugaugaac agcuccaucu cugaugcgaa gaugaaaggc     2880

uucaucaauc ggcagcuggu ggagaccagc caauccguua agcucacaca gaugaugcug     2940

ucucagaaau auccuggggc uaaggugaga cccauuaaag ccagcaucuc cagccagcuu     3000

cgcgacgccu gugaucuggu caagugccgg gaagcuaacg acuaccauca cgcccacgau     3060

gcguaucucg cuugucaagu gggaagauuu guucugaagc gccauucuuc cauguucgac     3120

aauccuauug gcaugacgaa agugauccgg gauuuuguca agagacaggg gcaggaguuc     3180

aaacggaacg gcaagcugcc cgccuacgcc agccgcucug gauuuauugu gcaguccuuc     3240

augagaagcg gcuuugacgc ugagaccggg gaacuucugc gggaugacug gaacgccccu     3300

uucgagaucg cucgcauuaa aagaugccuc aauuaucggg acuguuacau cucuagaaug     3360

cccauggaag auuccggagc cuuuugggac gcgacaaucu acagcccgaa ggccggcaaa     3420

aagggggcuc ugccugugaa gcaaggacuc cccguugaga aauauggcuc cuacagccgc     3480

gaacaguucg ccuacuuuuu cgucuauaag gcuuauaacc ccaaaaagaa agccuaccgg     3540

cuggaguucg aggccgugcc ugugagaguc gcuucugcgg uggaaacccg cgagaaugcc     3600

cuugaagagu augcuaagag cguugccaag caggccggcc uggaauuugu ggaggucgcu     3660

cggccgaaag uguacaagua ccaacucauu gagcuggggg aagauagacu guauaucaca     3720

ggaaaacgcg agguucggaa cgccucccag cuugcguucu cucuggacga aauugaucuc     3780

cugaagagac ucgcugaggg cgaagagacc agcuccgagg aacgcgagag acuguuugac     3840

gugcuuaucg aaaaauacgc ccgguaugcu ccccgccugg cuagacagcu cucugucgau     3900

agcauguccg ccacguucga gacccugaau gcggaggaac agcuggggau ucuucugucu     3960

cucguggaga ucgcuaacgg aaagcggaau augaucgacc ugagcuccgc cggcgggagc     4020

aagaacgccg gaugcaugaa uccuacauuu ucuuccgaac ucagcucucc cggcuccaac     4080

uucaccuuua uugaucaaag cguuacaggc auguucgagc gcagacagcg gcuggaacuu     4140

uga                                                                   4143


<210>  4369
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4369
auggugaaga aaaaguacgc caucggcauu gacaucggga ccaacagcgu cggauggucc       60

auuguuacag augacuauaa agugcccucu aagaaaauga agguguucgg caauacggag      120

aagagauaca ucaaaaagaa ccugcucggg acccugcuuu uugaugaagg aaauacagcu      180

gagaaccggc gccugaaaag aaccgcccgg cgcagauaua cacggcgcag aaaucggauu      240

cucuaccugc aggaaaucuu cgcggaggaa aucaacaaga uugacgauag cuuuuuccaa      300

cgccuugacg auuccuuucu gaucgucgag gacaaacagg gcucuaagca cccuauuuuc      360

gggacccucc aggaggaaaa ggaguaucau aaacaguuuc cgacaaucua ccaccugaga      420

aagcaacugg cugauagcuc ccagaaagcc gacauucggc ucaucuaucu ggcccuugcu      480

cauauuauca aguaccgcgg acacuuccug uuugaaggcg aucucaaauc ugagaauaag      540

gacgugcagc aucuguucaa cgauuuuguu gagauguucg acaaaaccgu ggaaggcagc      600

uaccuuuccg agaaucugcc caacgucgcc gacgugcucg uugaaaaggu gagcaagucu      660

agacggcugg agaauauccu gcacuauuuu ccuaacgaga aaaagaaugg acucuucgga      720

aacuuccugg cucuugcccu gggccuccag cccaauuuua aaacgaacuu cgagcuggcg      780

gaggaugcca agauucaauu uuccaaagaa accuacgagg aagaccuuga ggaacugcuc      840

gggaagaucg gagaugacua ugcugaucug uucauugcca caaagagccu guacgacggc      900

auccuccugg cugggauucu uucuaccaca gauuccacca cgaaagcccc ucugucuagc      960

uccaugguca accgcuauga ggagcaccag aaggaccucg cgcugcuuaa aaauuuuauc     1020

caucagaacc ugagcgauuc cuacaaggaa guguucaaug acaaacucaa ggauggauau     1080

gccggcuaca ucgagggcaa gacaacccag gaaaacuuuu acagauucau uaaaaaggcu     1140

aucgagaaaa uugaaggguc uaauuauuuu aucgacaaga uugaccggga ggauuuccug     1200

agaaaacaac gcacauuuga caacggaagc aucccccacc agauccaucu gcaggagaug     1260

cacgccauua uccggagaca agcugaauuu uacccuuuuc ucguggagaa ucaggauaag     1320

auugaaaaga uccugaccuu ccgcauuccg uauuacgucg gcccccuugc ccgggggaaa     1380

uccgaguucg ccuggcugaa cagaaagagc gacgagaaaa uccgcccuug gaauuuugau     1440

gaaaugguug acaaggagac cucugcugaa aacuucauua cacggaugac gaauuaugau     1500

agauaccucc ccgaccagaa agugcugccu aagcacuccc ugcuuuauga gaaauuugcg     1560

guguacaacg aacucaccaa ggucaaguuc aucgccgagg gaaugcgcga uuaccaguuu     1620

cuggacagcg gccagaaaaa ggauaucgug aaaacacugu ucaagaccaa acggaagguu     1680

acagcuaagg acauuaaagc cuaucucgaa aauucuaacg gguacgccgg aguggaacug     1740

aagggccuug aggaacaauu uaaugcuagc cugcccaccu aucaugaucu ccugaaaauc     1800

cugagagaca aggccuucau ugacgcggag gaaaaccagg agauccuuga ggauauuguc     1860

cucacgcuga cacuguuuga agaccgcgag augauccgga aaagacucga aaaguacaag     1920

gauauccuga ccgaggaaca gcggaaaaag cuggagcgca gacacuauac cggcuggggg     1980

cggcuuuccg cuaaacucau uaacggaauc cuggacaagg ugacaagaaa aaccauucug     2040

ggcuaccuua ucgaugacgg gacaucuaau cgcaacuuca ugcaacucau uaaugaugac     2100

acgcugagcu uuguugauga gauccggcug gcccagggau ccggcgaagc cgaggacuau     2160

agagcugaag ugcagaaccu cgccgggagc ccggcuauca agaagggcau ucugcagucu     2220

cuuaaaaucg ucgaugagcu gauugaggug augggauacg aaccugagca uaucgucgug     2280

gaaauggccc gcgagaauca auucaccaac cagggcagaa gaaauuccca gcagcgcauu     2340

aacagacucg aaaaugcgcu gaaggacuuu gggagcaaaa uccuggagga gcaaaagccc     2400

uacggaguuu cuaaagugaa caauauccac cuucaguccg aucgccucuu ccuguauuac     2460

cugcagaacg gcaaggacau guauacagac gaggaacucg auauugacca ucugagcgaa     2520

uacgauaucg accacauuau cccucaagcc uucauuaaag augacucccu ugauaauaag     2580

guccugacca agucugcuaa aaaccgggga aagagcgacg augugcccuc cauggagauc     2640

guucacaauc ggaaaaacuu uuggcggcag cuccugaagu cuaaacugau uagccagcgc     2700

aaguucgaca accuuacaaa ggccgaaaga gggggccuga ccaaugagga uaaagcucgg     2760

uuuauccagc gccaacucgu ggagacgaga cagaucacca agcaugucgc ccggaugcug     2820

gacacaagau ucaacaccaa acucgacgaa gcggggaauc gcauucggga ucccaaggug     2880

aacaucauua cacugaaauc caaucuugug agccaguuua gaaaggacua ucagcuguac     2940

aagauccgcg agauuaacaa uuaucaccau gcccacgaug cuuacaucaa cgccguuguc     3000

gcuaccgccc uccugaaaaa guacccucaa cuggccccgg aguucgugua uggcgaauac     3060

cccacguaua auucuuacaa cucccggaaa agcgcuacag aaaaggugcu uuucuauucu     3120

aauaucauga acuucuuuaa aagaauuguc gugcacucca agaccggaga gguucgcauc     3180

cggccuguga uugaggucaa uaaggaaaca ggcgagaucg ugugggacaa aaagagcgau     3240

uucagaaccg uucgcaaagu gcugucuuac ccccagguca acgugguuaa gaaaaccgaa     3300

gugcagaauc augggcucga ccggggaaag ccuaagggcu uuuauaacgc gaaccugagc     3360

cccagaccga aagagggguc cgucgagaau cucgugccug ccaagcagag cuucgauaca     3420

aaacgguacg gaggcuacgc ugggauuucu aacuccuaug ccgugcuggu caauggcauc     3480

aucgaaaagg ggaaaaagaa aagcaaggcc gagguuacgg aguuccaagg cauuuccauc     3540

cuugcucgca aggacuuuga uaaaaacccc aagcaguacc ugcucaaucu gggguauaaa     3600

gacauuaagu cuaucauuaa acugccuaag uacagccuuu ucgaacugga aaacggaucc     3660

agacggaugc ucgccucuau ccugagcacc aauaacaagc gcggcgagau ucacaaaggg     3720

aaugagaugu aucuccccga uaaguuugug acacugcuuu accaugcgau gagagugaac     3780

cggacccugg aaccuggaca caaaaaguau gucgagacac auagacacgu guucgacgaa     3840

cugcucaucu acauccugga guacaaugag aaaauuguug gcgcuaaggc caacgggaag     3900

cgcaucgugg aagccuauuc cgcucggaaa gauaccgaca aucuugagga acugugcaac     3960

ucuuuuauua aucuccugaa ccucacggcc cugggcagcg cggucgacuu cgaguuucuu     4020

ggaacaaaga uccccagaua ccgcgauuau accccgucca gccugcugaa agcuacccuc     4080

auucaccagu cugugacagg ccuguacgaa acccggaucg accuuuccaa gcugggaggg     4140

gauuga                                                                4146


<210>  4370
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4370
augaacuacg ugaaggagua ucacaucggc cuggacauug ggaccagcuc caucggaugg       60

gccgucacag auucucaguu caaacucaug agaauuaagg gcaaaacggc uaucgggguu      120

cggcuguuug aagagggaaa gaccgccgcg gaacgcagaa cauuccggac cacacgcaga      180

cggcuuaaac gcagaaagug gcggcugcau uaccucgacg agauuuuugc uccccaucug      240

caagaagugg augagaauuu ccuucgcaga cugaagcaga gcaacaucca cccugaggac      300

ccggccaaaa aucaggccuu uaucggcaag cuccuguucc ccgaucugcu caaaaagaac      360

gaacgggggu auccuacccu gauuaaaaug cgcgacgagc uucccgugga acagagagcu      420

cacuacccug ucacaaauau cuauaagcug cgggaggcca ugauuaacga ggaucgccaa      480

uuugaccuca gagaagugua ccuggcuguu caucacaucg ugaaguaucg gggacacuuc      540

cuuaauaacg ccuccgucga uaaauuuaag gugggcagaa uugacuucga uaaaucuuuu      600

aauguucuga acgaggcgua cgaagaacuc cagaauggcg aagggagcuu caccaucgag      660

cccuccaagg uggagaaaau uggacagcug cugcucgaca cgaagaugcg caaacuggau      720

cggcagaagg ccgucgcuaa guuucuugaa gugaaagugg ccgacaagga ggaaaccaaa      780

agaaacaagc aaaucgcuac agccaucucu aaacuggucc ucggcuacaa ggcggacuuc      840

gccaccguug cuauggccaa ugggaacgag uggaagauug aucugagcuc cgaaacaagc      900

gaggacgaga ucgaaaaauu ccgcgaggaa cuuucugaug cucagaauga cauucugacc      960

gagaucacgu cccucuuuag ccagauuaug cugaacgaaa ucgugccuaa cggaaugucu     1020

aucuccgaga gcaugaugga ucgguauugg acacaugaga gacagcuggc cgaagugaag     1080

gaguaccucg ccacccaacc ggcuucugcg cgcaaagagu ucgaccaggu cuauaauaag     1140

uacauuggcc aggcccccaa agagaagggg uuugaucugg agaagggacu uaaaaagauc     1200

cuguccaaaa aggaaaacug gaaagagauu gacgaacucc ugaaggcugg cgauuuccuu     1260

ccuaagcaac ggacaagcgc caaugugguu aucccccacc agaugcauca gcaggagcug     1320

gacagaauua ucgaaaaaca agccaaguau uacccuuggc ucgcuaccga gaaccccgcc     1380

accggcgagc gcgaucggca ccaggcgaaa uaugaacugg accagcuggu guccuuuaga     1440

aucccguacu acgucgggcc ucucgugaca cccgagguuc agaaggcuac gucuggagcc     1500

aaauucgccu gggcuaagcg caaagaagac ggcgagauua ccccuuggaa ucugugggau     1560

aagaucgacc gggccgaaag cgcugaggcc uuuauuaaga gaaugacagu gaaagauacc     1620

uaucuucuga acgaggacgu ccuccccgcc aauucccugc uguaccaaaa guauaacgug     1680

cuuaaugaac ucaacaaugu ccgggugaac gggcgcagac ugagcguugg aaucaaacag     1740

gauauuuacu cugagcuguu caagaaaaag aagacaguga aagcguccga cgucgcuagc     1800

cucgugaugg ccaagacccg gggcguuaau aaacccucug uggaagggcu gagcgauccu     1860

aagaaauuua acuccaaucu ugcuacguau cuggaccuca agucuaucgu cggagauaag     1920

guggacgaua acugcuacca gacagaccug gagaacauua ucgaauggag aagcguguuc     1980

gaggauggcg agaucuuugc cgacaaacug accgaaguug aguggcuuac cgacgaacag     2040

cgcuccgcgc ucgucaagaa acgguauaag ggcuggggga gacugagcaa aaagcugcuc     2100

acaggaauug uggaugagaa uggccaacgc aucauugacc ugauguggaa caccgaucag     2160

aauuucaugc agaucgugaa ccagccgguc uuuaaggagc aaauugacca gcuuaaucag     2220

aacgccauca caaaugaugg gaugacgcug agagaacggg uggagucugu ucucgacgau     2280

gcuuacacca gcccccaaaa caaaaaggcc aucuggcaag ugguccgcgu gguugaagac     2340

auugugaaag cugucggaaa ugccccuaag uccaucucua uugaguucgc cagaaacgaa     2400

ggcaauaaag gggagaucac acggucccgc agaacccagc ugcagaagcu guucgaggau     2460

caggcucacg aacuugugaa agacacaagc cucaccgagg aacuggagaa ggcgcuggau     2520

cucuccgacc gguacuaucu guacuuuacg cagggcggaa aggauaugua uaccggcgac     2580

cccauuaacu ucgacgaaau cucuacaaaa uacgauaucg accauauucu uccucagagc     2640

uuuguuaagg augacucccu ggauaaucgc gugcucgucu cuagagccga gaacaauaaa     2700

aagagcgacc gggugcccgc uaaacuguau gccgccaaga ugaagccgua cuggaaccag     2760

cugcuuaaac aagggcucau cacccagcgc aaguucgaga accugacaau ggauguggac     2820

cagaauauua aauauagauc ccugggauuu gucaagcggc agcucguuga aaccagacaa     2880

gugaucaaac ugacggcuaa cauucuuggc agcauguacc aggaggccgg gacagauauc     2940

aucgaaaccc gcgcgggacu gacaaagcag cuccgggagg aguucgaccu gccuaaggug     3000

agagaaguca augauuacca ccaugcugug gacgccuauc ugaccaccuu ugccggccaa     3060

uaccuuaacc gccgguaucc caaacugaga ucuuucuuug uuuacgggga guauaugaag     3120

uucaaacgcg gcuccgaccu caagcugcgg aauuuuaacu ucuuucacga acucauggag     3180

ggagauaaaa gccagggcaa gguggucgac cagcagacag gggaacugau uacgaccaga     3240

gaugaggugg cuaagucuuu cgaccgccuu cugaauauga aauacaugcu cguuuccaag     3300

gaggugcaug aucggagcga ccaacuguau ggagccacaa ucgucaccgc gaaagaaucu     3360

ggcaagcuga caagcccuau ugagaucaaa aagaacagac ccguggaucu uuacggggcu     3420

uacaccaaug gaacguccgc cuucaugaca auuaucaagu uuaccggcaa caaaccuaag     3480

uauaaaguua uuggggugcc caccacaagc gccgucggcc ugaagcgggu gggaaaaccg     3540

ggcucugaau ccuacaauca ggagcuccac cgcaucauca agagcaaccc uaaagugaag     3600

aaggacuucg aaauugucgu uccccacgug uccuauucuc agcugaucgu ggauggggac     3660

uguaaauuua cccucgcuag cgauacauac cagcaucccg ccacgcaacu gguccuuucc     3720

aaggagucua uggagauuau cgcugacgau uucaaauuug ugaaggaaaa uccugccacc     3780

gcggacgagc agcugguuag aguguucgac gaaauuguca accagaugaa ucacuauuuu     3840

acaaucuucg aucagcggag caaccgccaa aaagugacca aggccagaga caaguuuguu     3900

ucccucccga cagagucuga auacgaggga gcuaaaaaga cccagguggg caaaacggag     3960

gucaucacca accugcugau ggggcuucau gccaaugcug cccagggaga ucugaaggug     4020

cucggccuga gcacauucgg guuuuuccag uccagcggcg gacucaaccu gucugaagac     4080

gccaugauug ucuaucaauc ccccaccggc cuuuucgagc ggagaaucug ccugaaagau     4140

auuuga                                                                4146


<210>  4371
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4371
augaagcaga uccugaaaaa ggugcaaggc uacaacauug ggcucgacau cggaaccaau       60

agcgucggcu ggguugugac agaugagaac gccaaucugc uucaguucag aaagaagaac      120

auguggggag cucggcuguu ugaaaaagga gacacggccc agggccgcag acucucccgg      180

ucuacccgca gacgguauga ucgcagacgg cagcgcauuc acuaccugca agagaugcuu      240

gcgacaaugg ugcugcccau cgacgagagc uucuuuauua gacucaauga ggcuuuccug      300

uggaaggaag auaaguccgu ccagcgggac uauauccugu uugccgauaa acaguucacc      360

gaccgcgauu uuuacaagca guucaaaaca aucuaucauc ucagaaaguu ucugcuugac      420

accaaagaga aggccgaucc ucgguucauu uaccuggcuc uccaccauau gaucaaguau      480

cgcgggaacu uucuguacca aggacagucu uucgacgugg uugagaaugu gcaggagagc      540

uuuagagucc uucuggaaga aauguacgag cagcucggca aagaguggga auauggcgag      600

cacuucgaug acauucaacg gauccuggaa aacacaacca uugggaagaa auccaagcag      660

gagcagaucg ccgcuauucu gacggaacaa uacggaaaua aaaagcucgc caacgaggug      720

accagagcgu gccugggcua uucuuucgac cuuacaguug ugauggagaa ugaaaaccug      780

cucgaugaga gcgggaaaaa gaucaagauc uccuuuagcg acucuaaaua cgaggaacag      840

gaacuggagc uucagaaucg ccuggaggaa aaguucgccg ucauugaggc ucucaaacgg      900

aucuauaacu ggcuggugcu gaaggauacc cucagaggga aaaaguacau uuccgaagcc      960

auggugaaua aguaugaggc ucaucacgaa gaccugguca aacuuaagcg ccuguuucgg     1020

acauaccuca gcaccgagga guacucuugu uucuuuagaa aagaaaagca gaaagauaag     1080

uauaucucca acuacguuaa cuaucgcaag ggcgccgagc ggugcagcaa agaaaagcug     1140

uacaaagcga uucacgagcu uuucgacacg guggccaagg augacguggu cuaucaagag     1200

aucguggaaa agauggauca gggggaguuu cugacaaagc agaaugaagu uucuaacgcu     1260

uacaucccgu aucaguguaa ugagguggaa augaagagaa uccucaaaca acagggagag     1320

uucuaccccg agcugaagga aaacgccgac aaaauucugc agcuccugcu uuuucgcauu     1380

ccuuacuaug ucggcccccu gguggaugag aagcagucca aauucgcuug gaugaagcgg     1440

agaaccggca uggaacaagu uaaaaucuac ccuuggaauu uugaacaggu ggucgacauu     1500

gaccagacag ccgaagccuu caucacccgc augaccaacu auugcacaua ccuccccgag     1560

gagaaggugc ugccuaagca gagccugcuu uauuccgaau acgagcuccu gcaagaacug     1620

aauaaaauca agauuaacgg gaaauuuucu cucgagacgc ggaauaagau cauugaacag     1680

cuguucaaaa agcagaagaa agucaccgag aagcaacuua aaaaguggcu ggugagagag     1740

caguauugua caaccaaagu uacagaaaag auggugaagc ucgaugagaa cgcuuacuac     1800

aucaccggau uccagaaaga aaaugaguuu gcgagcuccc ugacgagcua uauugacuuc     1860

acaaagaucc ugggcgagau uaccgaacgg aaccgcaaaa ugaucgagga aaucauuuac     1920

uggcuuaccg ucuuugagga aaagucuauc gugaaaagaa agauucagaa gaaauauggg     1980

aaugagauca acgauaagca acucgcccag auucugaaac ugaauuacuc cggauggggc     2040

cgguucagcg agaaguuucu caacgggauc aaaggauucg acagacaggg ccagaagaaa     2100

acaaucaugg aacugcuucg cgaggauucu cggcugcuca acauuaugca aaucauuaau     2160

aacgaagaga ugggcuuuug caagaucauu gaagcuaauc agaagaaacu guaugggaac     2220

gagaaucugc uugacguugu gcaggagcuc cauggaagcc cggccaucaa gagaggcauc     2280

uggcaguccc ugaaaauugu cgaagagauc auuucuauca ugggaggagu gccccaaaac     2340

auuuucaucg aauuugcccg cgaggaugac gauagagugc ggaccaauag ccgcguuaag     2400

cagaucgaaa gacuguacaa aaaguucaag gaggagguca acgacuuuaa ugaacacgcu     2460

gccaacgagc ucaaagaaaa gcgguaucag aaucgcauug augaggagaa aguguuccug     2520

uacuuuaugc aaaacggcaa gucccuuuac agcgggguga cacuggacau cgauagacuc     2580

aaugaauaug aggucgacca uauuaucccu cggacgcugg ugaaagacga uucucuggaa     2640

aacaaggcgc uuguucucaa gggcgagaau cagcgcaaag gagaaggccu gauuccccac     2700

gaguggcaga acagacagaa ggaggugugg aaaaagcugc ucuccugugg gcugaugggg     2760

aaaaagaagu acgcuaaccu uugcaaagag agcuucggca augaaaagga acaaaaacac     2820

uucaucgccc ggcagauugu cgagacccgc cagaucauca aacagguggc caacauucug     2880

gcucaauauu accagggggu uaauguggcc gcugucaaag ccucucucgg auccgaacug     2940

agagagaacu auacaaucga gaagaaggac gauaauggcu uuuucaugga aaacaaaagc     3000

uacaccaugc ugaagucccg gaaaauuaau gacuuucauc acgcgcagga ugccuaucuu     3060

gcuugugccg uggggcugua caucaaggcu gccuucccua aauacgagaa ggaaauugac     3120

uaugagggcu acagaaaaua uuaccagaag aagaaccaac agaaagaugg aguucgcaau     3180

gaaaagcggc aguuuuucuc uuaugugaug agcagauuuu ccgagcagau cgucgacaaa     3240

gagacaggcg aaauccucug ggaugggacc caaaagcugg cguacauuaa aaagaucuuc     3300

ggauaucgcg acugccucau uacgaagaaa cuggaggaaa acaccggcga guuuuacaag     3360

cagacaauca augccaaacc cgagaagggg aaagaaaaca auaccaaacu ucugccgauu     3420

aagaaaggac ucucugugga gaaguacggc ggguaucagg gcggagaagc ugccuacagc     3480

ugucuggugc gguaucaaca gggcagaaaa acaaagcugu cccuugagaa aaucagcguc     3540

aucauugcua agaagaucgc cgaaaaagag guuucucugg aucgcuaccu caaggagaaa     3600

cugggggugg aagagauugu gauccuccgg aagaaaguca ugaagaacca gcugauugaa     3660

gacuccgaug gaaauagauu cuaucuuacc gccgcuacgg aggugguuaa cgcgaaacag     3720

uuuauccugg acggcaagca ucaacaccuc cugcgcacaa uugccaucau ggaaaagggg     3780

gaguacuaug gauaccggcu ggacgagcuu gaaaacagca uggaugagcu guacgcuuau     3840

uacagaauga aaauccagca gcaguauccu gccuacgaau cuauugugaa gaaaaucguc     3900

gagucccaug uguaugaaag ccuccacguu gaggagaaga uuaaagccgu gcaagaaaag     3960

ggcaaguucc ugauccagcu ccugcggcuu accgaggcua augccgaaug cauugaccug     4020

aaaacaaucu cugaugggcg ccuggcggac agaaucggcc ggaagaacca cuacaccauu     4080

aaagagggaa ucaaguucau ugaucagagc aucaccggcc ucuaugagcg cuacaugaca     4140

gucuga                                                                4146


<210>  4372
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4372
auggacuacg ugaaggagua ucacaucggc cuggauauug ggaccagcuc caucggaugg       60

gccgucacag acucucaguu caaacucaug agaauuaagg gcaaaacggc uaucgggguu      120

cggcuguuug aagagggaaa gaccgccgcg gaacgcagaa cauuccggac cacacgcaga      180

cggcuuaaac gcagaaagug gcggcugcau uaccucgaua agauuuuugc uccccaccuu      240

caagaggugg acgaaaacuu ccuucgcaga cugaaacaga gcaauaucca uccugaggau      300

ccggccaaga accaggccuu uaucggcaaa cuccuguucc ccgaccugcu caagaaaaau      360

gagcgggggu auccuacccu gauuaagaug cgcgaugaac uucccgugga gcagagagcu      420

cacuacccug ucacaaacau cuauaagcug cgggaagcca ugauuaauga ggaccgccaa      480

uuugaucuca gagaggugua ccuggcuguu caucacaucg ugaaauaucg gggacacuuc      540

cuuaacaaug ccuccgucga caaguuuaaa gugggcagaa uugauuucga caagucuuuu      600

aacguucuga augaagcgua cgaggaacuc cagaacggcg aggggagcuu caccaucgaa      660

cccuccaaag uggagaagau uggacagcug cugcucgaua cgaaaaugcg caagcuggac      720

cggcagaagg ccgucgcuaa acuucuggag gugaaggugg ccgacaaaga agagaccaag      780

agaaauaaac aaaucgcuac agccaugucu aagcucgucc ugggcuacaa ggcggauuuu      840

gccaccguug cuauggccaa cgggaaugaa uggaaaaucg accuuagcuc cgagacaagc      900

gaagaugaga uugagaaguu ccgcgaagaa cugucugacg cucagaacga uauccucacc      960

gaaauuacgu cccuguucag ccagaucaug cugaaugcca uugugccuaa cggaaugucu     1020

aucuccgaga gcaugaugga ccgguauugg acacaugaaa gacagcucgc cgaggugaaa     1080

gaguaccugg cuacccaacc ggcgucugcc cgcaaggaau uugaucaggu cuauaacaaa     1140

uacaucggcc aggugcccaa ggagaagggg uucgaccuug aaaaaggacu gaagaaaauu     1200

cucuccaaga aagagaauug gaaggagauc gaugaacugc uuaaggcugg cgacuuucug     1260

ccuaaacaac ggacaagcgc caacggcguu auuccccacc agaugcauca gcaggagcuc     1320

gauagaauca uugaaaagca agccaaauau uacccuuggc uggcuaccga gaaucccgcc     1380

accggggaac gcgaccggca ccaggcgaag uaugagcugg accagcucgu guccuucaga     1440

aucccguacu acgucggacc ucuggugaca cccgagguuc agaaagcuac gucuggcgcc     1500

aaguuugccu gggcuaaacg caaggaagau ggggagauca ccccuuggaa ccuuugggac     1560

aagauugauc gggccgaaag cgcugaggcc uucaucaaaa gaaugacagu gaaggacacc     1620

uaucugcuca augaagaugu ccugcccgcg aacucccugc uuuaccaaaa auauaaugug     1680

cucaacgagc ugaauaacgu ccgggugaau ggacgcagac ugagcguugg cauuaagcag     1740

gacaucuaca cagagcucuu uaaaaagaag aaaaccguga aggccgggga ugucgcuucu     1800

cuggugaugg ccaaaacgcg gggaguuaac aagcccuccg uggaaggccu uagcgacccu     1860

aaaaaguuca auucuaaccu ggcuacauau cucgaucuua agagcauuau gggcgacaaa     1920

gucgaugaca auagauacca gauggaucug gagaacauca uugaauggcg cuccguguuu     1980

gaggacgggg aaaucuucgc cgacaagcug accgaggugg aguggcucac cgaugaacag     2040

cggucugcgc ugguuaaaaa gagauauaaa ggauggggcc gccugagcaa gaagcuccug     2100

acagggaucg ucgacgagaa cggacaaaga auuaucgauc uuauguggaa uaccgaccag     2160

aacuuuaugc agauugugaa ucagccggug uucaaagaac aaaucgauca gcugaaccag     2220

aaggccauua caaaugacgg caugacgcuc cgggagcgcg ucgaguccgu gcuggaugac     2280

gcuuacacca gcccccaaaa caaaaaggcc aucuggcagg uugugagagu cguggaagau     2340

aucguuaaag cuguggggaa ugccccuaag ucuauuucca ucgaguuugc ccggaacgaa     2400

ggcaauaaag gagagauuac acgcagcaga cggacccagc ugcagaagcu guucgaagac     2460

caagcucacg agcucgucaa ggauacaucu cugaccgagg aacuggagaa agcgcccgac     2520

cucuccgauc gcuacuauuu cuacuuuacg cagggcggga aggacaugua uaccggagac     2580

ccuaucaacu ucgaugaaau uagcacaaaa uacgacaucg aucauauccu gccccagucc     2640

uuugugaagg acgauucucu ugacaauaga guucugguga gccgggccga gaacaauaaa     2700

aaguccgauc gcgucccggc uaagcucuau gccgccaaaa ugaagccuua cuggaaccag     2760

cugcugaaac aaggccuuau uacccagaga aaguucgaaa aucucacaau ggacguggau     2820

cagaacauca aauaucgguc ucugggguuu gugaagagac agcuggucga gacccgccaa     2880

guuauuaagc ucacggcuaa cauccuggga agcauguacc aggaggccgg cacagacauu     2940

aucgaaaccc gggcggggcu uacaaaacag cugagagagg aguucgaucu ccccaaggug     3000

cgcgagguga augacuacca ccaugcuguc gacgccuauc ugaccaccuu ugccggccaa     3060

uaccugaacc ggagauaucc uaaacuucgc uccuucuuug uguacggaga auauaugaag     3120

uucaaacacg gcagcgaucu gaagcuccgg aauuuuaacu ucuuucauga gcugauggag     3180

ggggacaagu cucagggaaa aguuguggau cagcagacag gcgaacucau cacgaccaga     3240

gacgaggucg cuaaguccuu cgaucgccug cuuaauauga aauacaugcu ggugagcaag     3300

gaaguucacg accggucuga ucaacucuau ggggccacaa uugugaccgc gaaagagucc     3360

ggaaagcuga caagccccau cgagauuaag aaaaacagac cugucgaccu guacggcgcu     3420

uacaccaaug ggacgucugc cuucaugaca aucauuaagu uuaccggcaa caaacccaag     3480

uauaaaguga ucggaguucc gaccacaagc gccgugggcc uuaagcgggu cgggaaaccu     3540

ggauccgaaa gcuacaauca ggagcugcac cgcauuauca agucuaaccc caaggugaaa     3600

aaggauuucg aaaucguggu cccccauguu uccuauagcc agcucauugu ggacggcgau     3660

ugcaaauuua cccuggcuuc cgacacauac cagcacccug ccacgcaacu cgugcugucu     3720

aaggagagca uggaaaucau ugcugaugac uucaaauuug ucaaggagaa uccggccacc     3780

gcggacgagc agcuugugag aguuuucgau gaaaucguga accagaugaa ucauuauuuu     3840

acaauuuucg accagcgguc caaccgccaa aaggucacca aagccagaga uaaguuugug     3900

ucucugccca cagagagcga auacgagggg gcuaaaaaga cccagguugg aaaaacggaa     3960

gugaucacca aucuccugau gggccugcac gccaacgcug cccaggggga ccuuaagguc     4020

cugggccucu ccacauucgg auuuuuccag ucuagcggcg ggcugaaccu cuccgaggau     4080

gccaugaucg uguaucaaag cccuaccgga cuguucgagc ggagaauuug ucuuaaggac     4140

aucuga                                                                4146


<210>  4373
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4373
augaagaaaa gcuacgccau cggcauugac aucgggacca acuccguggg acacugcguc       60

auguauacag agaauuacaa gguucccucu aaaaagauga aaguguucgg caacacggaa      120

aagagauaua uuaagaaaaa ucugcucggg acccugcuuu uugaugaggg aaacacagcu      180

gaaaaucggc gccugaagag aaccgcccgg cgcagauaca cacggcgcag aaaccggauc      240

cucuaucugc aggagauuuu cgcggaagag aucaauaaaa ucgacgauag cuuuuuccaa      300

cgccuugacg auuccuuucu gauuguggag gacaagcagg gcucuaaaca uccuaucuuc      360

gggacccucc aggaagagaa ggaauaccac aagcaguuuc cgacaauuua ucaucugaga      420

aaacaacugg cugauagcuc ccagaaggcc gacauccggc ucauuuaccu ggcccuugcu      480

cacaucauua aauaccgcgg acauuuccug uuugagggcg aucucaaguc ugagaacaaa      540

gacguccagc accuguucaa ugauuuugug gaaauguucg acaagaccgu ugagggcagc      600

uaucuuuccg aaaaccugcc caauguggcc gauguccucg uggagaaagu uagcaagucu      660

agacggcugg aaaacauccu gcacuacuuu ccuaaugaga agaaaaacgg acucuucggg      720

aauuuccugg cucuugcccu gggccuccag cccaacuuua agacgaacuu cgagcuggcg      780

gaagacgcca aaauccaauu uuccaaggag accuaugaag aggaccuuga agaacugcuc      840

gggaaaauug gagaugacua cgcugaucug uucaucgcca caaagagccu guaugacggc      900

auucuccugg cugggauccu uucuaccaca gauuccacca cgaaggcccc ucugucuagc      960

uccaugguga aucgcuacga ggaacaucag aaagaccucg cgcugcuuaa ggauuuuauu     1020

agacagaacc ugagcgacuc cuauaaagag uucuuuaaug auacacucaa ggacggauac     1080

gccggcuaca ucgaaggcaa aaccacacag gagaacuucu aucgguuuau caagaaggcu     1140

auugagaaaa ucgagggguc uaauuacuuc auugauaaga ucgacagaga ggacuuucug     1200

cgcaaacaac ggaccuucga uaacggaagc auuccccacc agauccaucu gcaggaaaug     1260

cacgccauca uuagacgcca agcugaguuu uauccuuucc ucgucgaaaa ucaggacaag     1320

aucgagaaaa uucugaccuu ccggaucccg uacuaugugg gcccccuugc cagagggaag     1380

uccgaguuug ccuggcugaa gcgcaaaagc gaugaaaaga uucggccuug gaacuucgac     1440

gagauggugg auaaagaaac aucugcugag aauuuuauca cgagaaugac caacuacgac     1500

caguaucucc ccgaucagaa gguccugccu aaacacuccc ugcuuuacga aaaguucgcg     1560

guuuacaaug agcucacaaa agugcgcuau gugaccgagc aaggaaagag cuuuuucuuu     1620

gacgccaaca ugaagcagga aauuuucgau ggcgucuuua aaguguaccg gaagguuaca     1680

aaagagaagc ugauggacuu ccuggggaaa gaauuugaug aguucagaau cguggaccuc     1740

augggacugg auaaggacaa uaagucuuuu aacgcuagcc uuggcaccua ucaugaccug     1800

aaaaagaucg ucuccaaaga ucuccuggac aaucccgaaa acgaggauau ucuggagaac     1860

gugguucuua cgcucacacu guucgaagac cgcgagauga uccggaagag acuggaaaaa     1920

uacaaggaug ugcucaccga ggaacagcgg aagaaacugg agcgcagaca cuauaccggc     1980

ugggggcggc uuucugccaa gcugauuaau ggaaucagaa acaaaacaac ccgcaagaca     2040

auucucgacu accugaucga ugacggcacg agcaaucgga acuucaugca gcugauccau     2100

gaugacaccc uuuccuuugu cgaugagauu agacucgccc aagggagcgg agaagcugag     2160

gacuaucgca cagaagugca gaaucuggcc ggcucuccgg cgaucaaaaa ggggauucug     2220

cagucccuca agaucgucga ugagcugauu ggcgugaugg gauacaaccc ugagcacguu     2280

guggucgaaa uggcuagaga gaaucaguuc accaaccaag gccggcgcag cucucagcag     2340

agauacaaaa agaucgaaaa ugccaucaaa aaccuugacu ccaaucugaa cagcaagauu     2400

cucaaagagu aucccacaaa uaaccaggcc cugcaaaaug accggcuguu ucuuuacuau     2460

cuccagaacg ggaaggauau guacaccgac gaggaacugg auaucgacca gcugucccaa     2520

uaugauauug accauaucau uccucaggcu uucaucaaag augacucucu cgauaacaag     2580

gugcugacca agagcgccaa aaaucgcgga aaguccgacg auguucccuc ucuugagauc     2640

gugcacaaaa agaaaaacuu uuggaagcag cugcucgaca gccagcugau uucccaaaga     2700

aaguucgaca aucugacgaa agcugaacgg ggcgggcuua caaacgagga uaaggcccgc     2760

uuuauccaga gacagcuggu cgaaacccgg cagauuacaa aacacguggc gcgcauccuc     2820

gacaccagau ucaauacgaa gcuggaugac gccggaaacc ggauuagaga ucccaaagug     2880

aauaucauua cacucaagag cucucugguu ucccaauuuc gcaaggacua ccagcuuuau     2940

aaaguccggg agaucaacaa uuaccaucac gcucaugaug ccuaccugaa cgcuguggug     3000

gccaccgcgc uccugaagaa auauccucag cuggccccgg aguucgucua cggcgacuau     3060

cccaaguaca auagcuauaa aucuagaaag uccgcuacag aacgcgugcu uuucuacagc     3120

aacaucauga auuucuucaa gcggguugug aaaucuagca agaccgggac cgucaaaauu     3180

agaccuauca uugaggugaa caaggaaaca ggcgagaucg uuugggauaa aaaguccgac     3240

uuucgcacgg ugcggaaggu ccugagcuau ccccagguga auguugugaa aaagaccgaa     3300

guccaaaacc acggacucga uagaggcaaa ccuaaggggu ucuacaacgc caaucugucu     3360

ccccgcccga aagagggauc cguggaaaac cucgugccug cuaagcagag cuuugacaca     3420

aaacgguacg gcggguaugc cggaauuucc aauucuuacg ccguccuggu uaacggcauc     3480

aucgagaagg ggaagaaaaa gagcaaagcu gaggugaccg aauuucaggg cauuuccauc     3540

cuugcgagaa aggauuuuga gaaaaauccc aagcaauauc ugcucaaccu gggauacaag     3600

gacauuaaau cuaucauuaa gcugccuaaa uauagccuuu ucgaacugga gaauggcucc     3660

cggcgcaugc ucgccucuau ccugagcaca aacaauaaga gaggggagau ccacaaagga     3720

aacgaaaugu accuccccga caaguuugug acccugcuuu aucaugcuau gcgggucaau     3780

cgcacgcugg agccuggcca caagaaauac guggaaacac auagacacgu uuucgaugag     3840

cugcucaccu acauucugga auauaacgag aagaucgugg gggccaaagc caauggaaag     3900

cggauugucg aggcuuacuc cgccagaaaa gacaccgaua accuugagga acuguguaau     3960

agcuuuauca accuccugaa ucucacagcg cugggcucug cuguggacuu cgaauuucuu     4020

gggaccaaga uuccccgcua ucgggauuac acaccgucca gccugcugaa ggccacgcuc     4080

auccaucagu cuguuaccgg ccuguaugag acaagaauug accuuuccaa acugggaggc     4140

gauuga                                                                4146


<210>  4374
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4374
augaagaaaa ucauuggccu ggaccucggg accacaagca ucggaugggc cuacgugcac       60

gaggcugaau ccgcccagga gagaucugaa auuguccggc ugggcgcgcg cguugugccc      120

cuuacgaccg augagcaaga cgaguucaag aaaggaaaga gcaucacaac caacgcugcc      180

agacugucca aacggucucu ccgcagaaau gccgaucggu uucagcugcg cagagacgag      240

cuugcuggag ugaugaagga ggccggcugg auugaugaaa caaccguccu ggcugagcag      300

gggccucgga gcacauucga aacccuccgc cugagagcca aggcggccuc cgaggaggug      360

acgcuggcug aauuugcccg gauccuccug cagaucaacc gcaaaagagg auauaagucu      420

aaucggaaaa ccgacggcga ugaggaaggc gcucuuauug acgggaugga uguuagcaag      480

caacugaacg ccuccggaca gacaccgggc gagugggugc ucgcgcgcau gcaggaaggg      540

aaugccguca uccccgcuuu cuacugcagc gaccugucug aggagcuuga uagaauuugg      600

cggaaacagc aagccuccca ucaggcuugg augaccgaag aacugcacac agugcucaga      660

caugccgacg gacgcagcgc cauccggcug auggauaagg cuggcguugu gccugacucu      720

gucaacguga aggaaagacg ccgggugaug uaugagugga gagcggccgc uguccgcgac      780

ugucugcccg ccgggcgggu ugccgcugcc cucaugcaag uguccaaaga aauugggaga      840

gcggauagcu accugggccg caucggcgac cggucuaagg agcuuuauuu ucacagacag      900

accguggggc aguaccugug gcgccucauu ugcgauaauc cuaaccaguc ccugaaaaau      960

cuugucuucu aucggcagga cuaccaacau gaguuugaaa cgcuguggag cauccagaca     1020

aaguaccacg uggagcucac cgaagaucug agaaaacggg uuggacgcau cauuuucuuu     1080

cagagacccc ugaaguccaa aaagggccuc gugaaccggu gugagcuggu ccacuauucu     1140

gugugcgaaa auagcgggga cgaucuuaca gagaagguug acgugggacc uaaagucugu     1200

ccgaagucca gcccccuguu ccaggaguuu aaaguguggc aaguccucaa caauguggaa     1260

guuauccauc cuucuaccca ggaggcuaga cgccuggaaa uggaggagaa ggaacuucug     1320

gccuccgagc uccuguuccg ggauagaaug aaaaagauug acgugcugaa gcuccuguuu     1380

agcaacuggc gcgaucuuga ccugaauuac aaagaacucg agggcaacag aaccauggcc     1440

gcuuucuauc gggucuacga acaggugauc gcccagacag ggcacggaga cuaugauuuc     1500

gcuaagaugc gcgccgcgaa aguuaaggag cuggccgagg gcguguuugc uggccugggg     1560

uucgaagucg gacuucucac guuugacgcc gagcuggauu cucccgcuua cgaacaacag     1620

ccuugcuaua gacuguggca ucuccuguac agcuacgagg gcgacaaauc cgccaccggg     1680

aacaaguguc uucugaagaa acucugccgg cugacaggaa ugaaugcgga acuggugggc     1740

cuuuucaugc agauucgcuu uucuggggau uauggcagcc ucuccaccaa ggccaugaga     1800

cggauccugc ccuaccugaa agcuggaaac acauaugacg uggccuguga guacgcuggc     1860

uuccgccaca gcgccagauc ucucacccgg gaggaacugg cccagaagga guauaaagau     1920

uaccuugacc ugcucccgcg caauucccug agaaacccug uugucgaaaa gauccugaau     1980

caaaugauuc acguggugaa cgggcuuagc ucuacguaug gaaagcccga ugagauccgg     2040

gucgaaaugg cuagagagcu caaaaagucc gcgcgcgagc gggaagagau gacaagcgcc     2100

auuuccaaaa ccaccaagga aaaugcugag aucacagcca uucugcagag agacuuuggc     2160

cugcgcuacg ugucucggaa cgauauccuc agauacaaac uguauugcga gcuucgcauc     2220

aaggggaaug gaugucggac ccuguacagc aacacauaua uuaauccuaa cgaacucuuc     2280

uccagagagu uugacaucga acauauuauc ccccagucuc gccuguucga ugacagcuuu     2340

uccaauaaaa cgcuggagag ccggaccgcc aaccuugaaa agggcgacag aacagcuugg     2400

gauuacguua augagaagua uggggccgag cgggcggacg aauaccgcgc ucagguggag     2460

gcccucuaua gagauggcac cauuucuaaa gccaagcggg acaaacugcu gaugcccgga     2520

gauaagaucc cugaagacuu cauugaucgc gacaucagaa acacacaaua caucgcuaaa     2580

aaggcccucg agauuuuuga agauuucguc ccguccgugg uugcgaccag cggcaacgug     2640

acggcucggc ugagagagga cugggggcuu gucgauguga ugaaggagcu gaauuggucu     2700

aaauacgaau cccagggaau gguucacauc gaguaugacc gcgauggccg gcccauuaga     2760

cgcaucgugg acuggaccaa gcggaacgac cauagacacc augccaugga ugcccucgcu     2820

gucgccuuca caaaacgcag cuacauucag uaucugaauc accugaacgc ucggaaggaa     2880

gccaccucug uggcguacgc cauccaacac aaagagaugg aacuugacgg gagcaauaga     2940

uggcgcuuua agugcccuuu cggagauaga uccgcuuuuc ggcgcgaggc caaggcucag     3000

cuggagagaa uccucgugag cauuaaaucu aagaacaaag uccugacacc cuccguuaau     3060

cggaccaaga aacgcggcgg guucaacaag aagguggaau ggacgccuag aggcgcccuc     3120

cauaaugaga caguguaugg acggaucaga cgcuacgacg ucaaagaacu gcgggugggc     3180

agcuccuuua ccgccgaggu uauugggaag guggcugauu cuagauaucg cgcggcccuu     3240

augcaccggc uggcugaguu cggaggcgac cccaaaaagg ccuuuacagg gaaaaacagc     3300

cucgaaaaga auccgcugua caccgaugag gcccauaccg cuugcguccc uuccucugug     3360

aaaguuguga gcuucgucga aaucggaaca auuagaaagg gcguggaccc caaccugaau     3420

guugauaagg uggucgacac gcacaucaaa aacauccuuc uggcccggcu cuccgcguac     3480

gggggcgaug cuaagaaagc cuuuucuaau cuggacgccg acccuauuug gcucaacaag     3540

gagaaaggaa ucgcuauuaa gcgcgugacc aucagcgaug acuccgaagu gagacccauu     3600

cacgucaagc gggauaugcg cggcagacug guuugugacg agcaugggcg gccugugccc     3660

agcgauuaug ugcagcuucg caacaaucac caugucgcca ucuucagaga cgcgaacgga     3720

gagcugaaag aacggguggu uacauuuuuc gaggcugugg aacgcgucag acagcuccgg     3780

ucugagggca gagccgugga aguuaucgau aagaauuaca aaugcgagga gggguggacc     3840

uuucuguucu ccaugaagau uaacgaauau uucguguuuc cggacgccgc uagcggauuc     3900

gauccucgcg agaucgaccu gacagauccc gccaauuacg cucuuauuuc uccccaccug     3960

uuucgggucc aaaaacucuc cagcaaggac uauuacuuca gacaucaccu ggaaaccacg     4020

gugaacgaug ucaagcagcu ccgcgacauc acauggaaac ggauuucuuc ccugcaggac     4080

cuugccggca ccgugaaggu uagagugaau cacaucggcc agauugucgc ggugggggag     4140

uauuga                                                                4146


<210>  4375
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4375
auggugaaga aaaaguacgc caucggcauu gacaucggga ccaacagcgu cggauggucc       60

auuguuacag augacuauaa agugcccucu aagaaaauga agguguucgg caauacggag      120

aagagauaca ucaaaaagaa ccugcucggg acccugcuuu uugaugaagg aaauacagcu      180

gagaaccggc gccugaaaag aaccgcccgg cgcagauaua cacggcgcag aaaucggauu      240

cucuaccugc aggaaaucuu cgcggaggaa aucaacaaga uugacgauag cuuuuuccaa      300

cgccuugacg auuccuuucu gaucgucgag gacaaacagg gcucuaagca cccuauuuuc      360

gggacccucc aggaggaaaa ggaguaucau aaacaguuuc cgacaaucua ccaccugaga      420

aagcaacugg cugauagcuc ccagaaagcc gacauucggc ucaucuaucu ggcccuugcu      480

cauauuauca aguaccgcgg acacuuccug uuugaaggcg aucucaaauc ugagaauaag      540

gacgugcagc aucuguucaa cgauuuuguu gagauguucg acaaaaccgu ggaaggcagc      600

uaccuuuccg agaaucugcc caacgucgcc gacgugcucg uugaaaaggu gagcaagucu      660

agacggcugg agaauauccu gcacuauuuu ccuaacgaga aaaagaaugg gcuguucgga      720

aacuuccugg cucuugcccu gggccuccag cccaauuuua aaacgaacuu cgaacuggcg      780

gaggaugcca agauucaauu uuccaaagaa accuacgagg aagaccuuga ggaacugcuc      840

gggaagaucg gagaugacua ugcugaucug uucauugcca caaagagccu guacgacggc      900

auccuccugg cugggauucu uucuaccaca gauuccacca cgaaagcccc ucugagcagc      960

uccaugguca accgcuauga ggagcaccag aaggaccucg cgcugcuuaa aaauuuuauc     1020

caucagaacc ugagcgauuc cuacaaggaa guguucaaug acaaacucaa ggauggauau     1080

gccggcuaca ucgagggcaa gacaacccag gaaaacuuuu acagauucau uaaaaaggcu     1140

aucgagaaaa uugaaggguc uaauuauuuu aucgacaaga uugaccggga ggauuuccug     1200

agaaaacaac gcacauuuga caacggaagc aucccccacc agauccaucu gcaggagaug     1260

cacgccauua uccggagaca agcugaauuu uacccuuuuc ucguggagaa ucaggauaag     1320

auugaaaaga uccugaccuu ccgcauuccg uauuacgucg gcccccuugc ccgggggaaa     1380

uccgaguucg ccuggcugaa cagaaagagc gacgagaaaa uccgcccuug gaauuuugau     1440

gaaaugguug acaaggagac cucugcugaa aacuucauua cacggaugac gaauuaugau     1500

caguaccucc ccgaccagaa agugcugccu aagcacuccc ugcuuuauga gaaauuugcg     1560

guguacaacg aacucaccaa ggucagauac gugacagagc aaggaaagag cuucuuuuuc     1620

gaugccaaua ugaaacagga gaucuuugac cgcaccuuca agguuuaucg gaaagugaca     1680

aaggaaaaac ugauggauuu ucugucuaag gaguucgacg aauuuagaau cgucgaucuc     1740

cugggccuug acaaggacaa caaaucuuuc aaugcuuccc uggggaccua ccaugaucuc     1800

aagaaaauug ugucugagga cuuucuggau aacagcgaaa acgaggacau ccuggagaau     1860

guugugcuua cgcucacacu guucgaagau cgcgagauga uucggaagag acuggaaaaa     1920

uauaaggacg uccucaccga ggaacagcgg aagaaacugg agcgcagaca cuacaccgga     1980

uggggccggc uuuccgccaa gcugaucaac ggcauuagag auaaagugac acgcaagacc     2040

auccucgacu aucugaucga ugacgggaca agcaaucgga acuucaugca gcugauuaau     2100

gaugacacgc uuucuuuugu cgaugagauc agacucgccc aaggauccgg caaagcugaa     2160

gacuaccgcg ccgaggugca gaaccuggcg gggagccccg cuauuaagaa gggaauccug     2220

cagucucuca aaauuguuga cgaacugauc gaggugaugg gcuaugaucc ggagcauauc     2280

gucguggaaa uggccagaga gaaucaguuc accaaccaag ggcggcgcaa uucccagcag     2340

agauacaaga aaauugaaaa cgccaucaag aaucuugaca gcaaccucaa uuccaaaauu     2400

cucaaggagu acccuacaaa caaucaggcu cugcaaaacg aucggcuguu ucuuuauuac     2460

cuccagaacg gcaaagacau guauaccgau gaggaacugg acaucgauca gcugucucaa     2520

uacgacauug aucacaucau cccccaggcc uucauuaagg acgauagccu cgacaauaag     2580

guucugacaa aauccgcuaa gaaccgcgga aaaucugacg augugccuag ccuugagauc     2640

gucuauaaga aaaagaauuu uuggcagcag cugcucgacu cccaacugau uagccagaga     2700

aaguucgaua accugaccaa agccgaacgg ggcgggcuua cgaaugagga caaggcgcgc     2760

uuuauccaga gacagcuggu ggaaacccgg caaauuacaa aacauguggc ccgcauccuc     2820

gauaccagau ucaacacaaa gcuggacgag gcuggaaauc ggauuagaga ucccaaaguu     2880

aacaucauca uucucaaguc uaaucugguc ucccaguuuc gcaaggacua ccagcuuuau     2940

aaagugcggg aaaucaacaa uuaccaccac gcccaugaug cuuaccugaa cgccgugguc     3000

gcgaccgccc uccugaagaa auauccccag cuggcuccug aguucgugua cggcgaguau     3060

ccgacguaca auagcuauaa cucuagaaag uccgccacag aaaaaguucu uuucuacagc     3120

aauauuauga acuucuucaa gcgcaucgug guccacucua agaccgggga ggugcggauu     3180

agacccguua ucgaagugaa caaagagaca ggagaaaucg ucugggacaa gaaaagcaau     3240

uuucgcaccg ugcggaaggu ucuguccuau ccucaaguga acgucgugaa aaagaccgag     3300

gugcagaauc auggccucga uagagggaag cccaaaggcu ucuacaacgc uaaucugagc     3360

ccucgcccca aggagggauc ugucgaaaac cucguuccgg ccaaacaguc cuuugacaca     3420

aagcgguacg gcggguaugc cggaauuagc aauuccuacg cugugcuggu gaacggcauc     3480

auugagaaag ggaagaaaaa gucuaaggcg gaagucacgg aguuccaagg aaucagcauu     3540

cuugccagaa aagauuuuga caagaauccu aaacaguauc ugcucaaccu gggcuacaag     3600

gacaucaaau ccaucauuaa gcugcccaag uauucucuuu ucgagcugga aaaugggagc     3660

cggcgcaugc ucgcuuccau ccugucuacc aacaauaaaa gaggcgagau ucacaaggga     3720

aacgaaaugu accucccuga uaaauuugug acacugcuuu aucacgccau gcggguuaau     3780

cgcacccugg agcccggcca uaagaaauac guggaagccc acagacaugu cuucgacgag     3840

cugcucacau acauccugga guauaacgaa aagauugugg gggcuaaggc caauggaaaa     3900

cggaucguug aggcguacag cgcuagaaag gauaccgaca accuugagga acugugcaac     3960

uccuuuauua aucuccugaa ccucacggcc cugggcagcg ccguggauuu cgaauuucuu     4020

gggacaaaaa ucccucgcua ucgggacuac acccccucuu cccugcugaa ggcuacccuc     4080

auccaccaga gcgucacagg acuguaugag accagaauug aucuuucuaa acugggcggg     4140

gacuga                                                                4146


<210>  4376
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4376
auggugaaga aaaaguacgc caucggcauu gacaucggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa agugcccucu aagaaaauga aggucuucgg caauacggag      120

aagagauaca uuaaaaagaa ccugcucggg acccugcuuu uugaugaagg aaauacagcu      180

gagaaccggc gccugaaaag aaccgcccgg cgcagauaua cacggcgcag aaaucggauc      240

cucuaccugc aggaaauuuu cgcggaggaa aucaacaaga ucgacgauag cuuuuuccaa      300

cgccuugacg auuccuuucu gauuguggag gacaaacagg gcucuaagca cccuaucuuc      360

gggacccucc aggaggaaaa ggaguaucau aaacaguuuc cgacaauuua ccaccugaga      420

aagcaacugg cugauagcuc ccagaaagcc gacauccggc ucauuuaucu ggcccuugcu      480

cauaucauua aguaccgcgg acacuuccug uuugaaggcg aucucaaauc ugacaauaag      540

gauguucagc aucuguucaa cgaguuugug gagauguucg acaaaaccgu cgaaggcagc      600

uaccuuuccg agaauauccc cgacguggcc gauauccugg uugaaaaggu gagcaagucu      660

agacggcucg agaacguccu gcaguauuuu ccuaaugaga aaaagaacgg gcugcucgga      720

aauuuccugu cccuugcucu gggccuccaa cccaacuuca aaacgaauuu ugagcuggcc      780

gaggacgcga agauucaguu cagcaaagaa accuacgagg aagaucuuga ggaacugcuc      840

gggaagaucg gagacgauua ugccgaccug uuuauugcua caaagucucu guacgauggc      900

auccuccugg ccgggauucu uuccaccaca gacagcacca cgaaagcucc ucugucuucc      960

agcaugguga accgcuauga ggagcaccag aaggaucucg cccugcuuaa agacuucauc     1020

agacagaacc uguccgauuc uuacaaggaa guguuuaaug acgcgcucaa agacggauau     1080

gccggcuaca ucgagggcaa gacaacccaa gaagauuucu acaaguuugu caaaaagaca     1140

auugagaaaa ucgaagggag cgacuauuuc auugauaaga ucgaccggga ggauuuucug     1200

agaaaacagc gcaccuucga caacggaucc auuccccacc agauccaucu gaaggagaug     1260

caagcuauca uuauccggca ggccaaguuu uacccuuucc ucgaagagaa ucaggauaaa     1320

auugaaaaga uccugaccuu ucgcauuccg uauuacguug gcccccuugc uagagggaac     1380

agcgaguucg ccuggcugaa ucggaaaucu gacgagaaga ucagaccuug gaacuucgau     1440

gaaauugugg acaaagagac auccgccgaa aauuuuauca cgcgcaugac caacuaugau     1500

caguaccucc ccgaccaaaa ggugcugccu aaacacagcc ugcuuuauga gaaguucgcu     1560

gucuacaaug aacucacaaa ggugcgguac guuaccgagc agggaaaauc uuucuucuuu     1620

gaugcgaaca ugaagcagga gaucuucgac ggcguguuua aagucuauag aaaggugaca     1680

aaagaaaagc ugauggacuu ccuggggaag gaguuugaug aauuucgcau uguugaccuc     1740

cugggacuug auaaagacaa uaagagcuuu aacgccuccc ugggcaccua ccaugaucuc     1800

aaaaagaucg ugucuaaaga ccugcuggau aaucccgaga acgaagacau ucuugagaau     1860

gucgugcuca cgcugacacu guucgaggau cgggaaauga ucagaaagcg ccucgagaag     1920

uauaaagaag uccugaccga ggaacagcgg aagaaacuug agagacggca cuacaccggc     1980

ugggggcgcc ugagcgcuaa gcucauuaac ggaaucagag acaaagugac acggaagacc     2040

auccuggauu aucugauuga cgauggcaca uccaacagaa auuuuaugca acuuaucaac     2100

gacgacacgc ucagcuucgu ugaugagauu cgccuggccc aggggucugg agaagccgag     2160

gacuaccggg cugaagugca gaaucuggcc ggcuccccgg cgaucaagaa agggauucuc     2220

cagagccuga agaucgucga ugagcuuauc gaggugaugg gcuaugaacc ugagcacauu     2280

guuguggaaa uggcuagaga gaaccaauuc accaaucagg gacgcagaaa cucucagcag     2340

cgguacaaaa agaucgaaaa ugccauuaaa aaccuggacu ccaaucucaa cagcaagauc     2400

cugaaagagu accccacaaa uaaccaagcc cugcagaaug aucgccuuuu ucucuauuac     2460

cugcagaacg gcaaggacau guauaccgau gaggaacugg acauugauca acucucccag     2520

uacgacaucg aucauaucau uccucaggcu uucaucaagg acgacucucu ggauaauaaa     2580

guccuuacaa agagcgccaa aaacagaggg aaguccgacg augugcccuc ucuggagauu     2640

guguauaaaa agaaguccuu uuggaaacag cuccuggaca gccaacugau cagccagcgg     2700

aaguucgaua accuuaccaa agcugaacgc ggaggccuga cgaaugagga caaggccaga     2760

uuuauucagc ggcagcucgu ugaaacccgc caaaucacaa aacacgucgc gagaaugcug     2820

gauacccggu ucaacacaaa gcucgacgag gccgggaauc gcauuagaga ucccaaggug     2880

aacaucauca cccugaaauc uaaucuugug ucccaguuuc ggaaggacua ccagcuguau     2940

aaagucagag agauuaacaa uuaccaucac gcucaugaug ccuaccucaa cgcugugguu     3000

gccacggcgc ugcugaagaa auauccucag cuugccccgg aguucgugua cggagaauau     3060

cccacauaca auagcuauaa cucucgcaag uccgcuaccg aaaagguccu guuuuacagc     3120

aauaucauga acuucuuuaa acggauugug guucacucua agacaggcga ggugagaauc     3180

cgcccuguca uugaagugaa uaaagagacc ggggagaucg uuugggacaa gaaaagcgau     3240

uuccggaccg ugagaaaggu ccucuccuau ccccaaguga acguggucaa gaaaacagaa     3300

guucagaauc acggccugga ccgcggaaag cauaaaggcu ucuacaacgc caaccucagc     3360

ccucggccca aggagggguc uguggaaaau cuggugccgg cuaaacaguc cuuugacacg     3420

aagagauacg gaggcuaugc cgggaucagc aacuccuacg ccguccuugu gaauggaauu     3480

aucgagaaag gcaagaagaa aucuaaggcu gagguuaccg aguuccaagg gauuagcauc     3540

cuggcgcgca aagauuuuga caagaacccu aaacaguauc uccugaaucu gggcuacaag     3600

gauauuaagu ccaucaucaa acuucccaag uauucucugu ucgaacucga aaacggaagc     3660

cggagaaugc uggccuccau ucucucuaca aauaacaaac ggggcgagau ccacaagggg     3720

aaugaaaugu accugccuga caaauuugug acccuucugu aucaugcuau gcgcgucaac     3780

agaacacugg agcccggaca caagaaguac guggagaccc accggcaugu uuucgaugaa     3840

cuccugauuu acauccuuga guauaaugaa aagauugugg gcgccaaagc caacgggaaa     3900

cgcaucgucg aggcuuacag cgccagaaag gacacggaua aucuggaaga acucugcaac     3960

uccuuuauua aucugcucaa ccugacagcg cuuggaagcg cuguggacuu cgaguuucug     4020

ggcaccaaaa ucccucggua uagagauuac acccccucuu cccugcucaa ggccacacug     4080

auccaccaga gcguuaccgg gcuuuaugaa acacgcauug accugucuaa gcucggcgga     4140

gauuga                                                                4146


<210>  4377
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4377
auggugaaga aaaaguacgc caucggcauu gacaucggga ccaacagcgu cggauggucc       60

guugugacag augacuauaa agugcccucu aagaaaauga aggucuucgg caauacggag      120

aagagauaca uuaaaaagaa ccugcucggg acccugcuuu uugaugaagg aaauacagcu      180

gagaaccggc gccugaaaag aaccgcccgg cgcagauaua cacggcgcag aaaucggauc      240

cucuaccugc aggaaauuuu cgcggaggaa aucaacaaga ucgacgauag cuuuuuccaa      300

cgccuugacg auuccuuucu gauuguggag gacaaacagg gcucuaagca cccuaucuuc      360

gggacccucc aggaggaaaa ggaguaucau aaacaguuuc cgacaauuua ccaccugaga      420

aagcaacugg cugauagcuc ccagaaagcc gacauccggc ucauuuaucu ggcccuugcu      480

cauaucauua aguaccgcgg acacuuccug uuugaaggcg aucucaaauc ugagaauaag      540

gacguucagc aucuguucaa cgauuuugug gagauguucg acaaaaccgu cgaaggcagc      600

uaccuuuccg agaaucugcc caacguggcc gacguucucg uggaaaaggu cagcaagucu      660

agacaccugg agaauauccu gcacuauuuu ccuaacgaga aaaagaaugg gcuuuucgga      720

aacuuccugg cucuugcccu gggccuccag cccaauuuua aaacgaacuu cgaacuggcg      780

gaggaugcca agauccaauu uuccaaagaa accuacgagg aagaccuuga ggaacugcuc      840

gggaagauug gagaugacua ugcugaucug uucaucgcca caaagagccu guacgacggc      900

auucuccugg cugggauccu uucuaccaca aacuccacca cgaaagcccc ucugagcagc      960

uccaugguga aucgguauga ggagcaucag aaggaucucg cgcugcuuaa aaacuuuauu     1020

caccagaauc ugagcgacuc cuacaaggaa guguucaacg auaaacucaa ggacggauau     1080

gccggcuaca ucgagggcaa gacaacccag gaaaauuuuu accgcuucau caaaaaggcu     1140

auugagaaaa ucgauggguc uaacuauuuu auugaaaaga ucgagagaga ggacuuccug     1200

cggaaacaaa gaacauuuga caauggaagc auuccccauc agauccaucu gcaggaaaug     1260

caagccauca uucgccggca ggcuaaguuc uacccuuuuc ucgaggaaaa ccaggauaag     1320

aucgagaaaa uucugaccuu cagaaucccg uauuacgucg gcccccuugc ccgcgggaag     1380

uccgaguucg ccuggcugaa ucggaaaagc gacgaaaaga uuagaccuug gaacuuugau     1440

gagaugguug acaaagaaac cucugcuaag aauuucauca cacgcaugac gaacuaugau     1500

caguaccucc ccgaccaaaa agugcugccu aagcacuccc ugcuuuauga gaaguuugcg     1560

guguacaaug aacucaccaa aguccgguac gugacagagc agggaaagag cuucuuuuuc     1620

gaugccaaca ugaaacagga gauuuuugac ggcguuuuca agguguauag aaaagucacc     1680

aaggaaaagc ugauggauuu ucuggggaaa gaguucgacg aauuucgcau cguggaucuc     1740

cugggacuug acaaggagaa uaaaucuuuc aacgcuagcc ugggcacaua ccacgaucuc     1800

aagaaaaucg uuuccaagga cuuucuggac aacucugaaa augaggauau ucuggagaac     1860

gugguccuua cccucacgcu guucgaagac cgggagauga ucagaaagcg ccuggaaaaa     1920

uauaaggaua uucucacaga ggaacagcgg aaaaagcugg agagacggca cuacaccggc     1980

ugggggcgcc uuagcgccaa acugaucaau ggaauuagag acaaggugac ccggaagaca     2040

auccucgauu aucugaucga cgauggcacc uccaacugca auuucaugca acugauuaac     2100

gacgauacac uuagcuuugu cgacgagauc agacucgccc aggggucugg agaagcugag     2160

gauuaccgcg ccgaagugca gaaucuggcg ggcucccccg cuauuaaaaa ggggauccug     2220

cagagccuca aaauuguuga cgagcugauc gaggugaugg gcuaugaucc ggaacauauc     2280

gucguggaga uggcccggga aaaccaauuc acgaaucagg gaagacgcaa cucucagcag     2340

agauacaaga aaauugagaa ugccaucaag aaccuugacu ccaaucugaa cagcaaaauu     2400

cucaaggaau acccuaccaa uaaccaagcu cugcagaacg accggcuguu ucuuuauuac     2460

cuccagaaug gcaaggauau guauacagac gaggagcugg auaucgacca acugucccag     2520

uacgauauug accacaucau cccccaggcc uucauuaaag augacucucu cgauaacaag     2580

guucugacca aaagcgcuaa gaaucgcggg aaauccgacg augugccuuc ucuugaaauc     2640

gucuauaaga agaaaucuuu uuggaagcag cugcucgacu cccaacugau uagccagaga     2700

aaauucgaca accugacaaa ggccgagcgg ggaggccuua ccaaugaaga uaaagcgcgc     2760

uuuauccaga gacagcuggu ggagacgcgg caaauuacca agcauguggc ccgcaugcuc     2820

gacacaagau ucaacaccaa gcuggaugaa gcugggaauc ggauccgcga ccccaaaguu     2880

aacauuauca cacucaaguc uaaucugguc ucccaguuua gaaaagauua ccagcuuuau     2940

aaggugcggg agaucaacaa uuaccaccac gcccaugacg cuuaccugaa cgccgugguc     3000

gcgaccgccc uccugaaaaa guauccccag cuggcuccug aguucgugua cggagaauau     3060

ccgacguaca auagcuauaa cucuagaaag uccgccacag agaaaguucu cuuuuacagc     3120

aauauuauga acuucuucaa gcgcaucgug guccacucua aaaccggcga agugcggauu     3180

agacccguua ucgaggugaa caaggaaaca ggggagauug ucugggauaa aaagagcgac     3240

uuucgcaccg ugcggaaggu ucuguccuau ccucaaguga augucgugaa aaagaccgag     3300

gugcagaacc auggccucga uagaggaaaa cccaagggcu ucuacaaugc uaaccugagc     3360

ccucgcccca aagaaggguc ugucgagaau cucguuccgg ccaagcaguc cuuugacaca     3420

aaacgguacg gaggcuaugc cgggaucagc aacuccuacg cugugcuggu gaauggaauc     3480

auugaaaagg gcaagaaaaa gucuaaagcg gaggucacgg aguuccaagg gaucagcauu     3540

cuugccagaa aggauuuuga caaaaacccu aagcaguauc ugcucaaucu gggcuacaag     3600

gauaucaaau ccauuaucaa gcugcccaaa uauucucuuu ucgaacugga gaacggaagc     3660

cgccggaugc ucgcuuccau ccugucuacc aauaacaaga gaggcgaaau ucacaaaggg     3720

aaugagaugu accucccuga caaguuugug acacugcuuu aucacgccau gcggguuaac     3780

cgcacccugg aacccggaca uaagaaauac guggagacac acagacaugu cuucgacgag     3840

cugcucaucu acauucugga auauaaugag aagaucgugg gcgccaaagc uaacgggaag     3900

cggauuguug aagccuacag cgcgcgcaaa gauaccgaca aucuugagga acuguguaac     3960

uccuuuauca accuccugaa ucucacggcu cugggaagcg ccguggauuu cgaguuucuu     4020

ggcacaaaga uuccuagaua ucgggacuac acccccucuu cccugcugaa ggccacccuc     4080

auccaccaga gcgucacagg gcuguaugag accagaaucg aucuuucuaa acugggcgga     4140

gacuga                                                                4146


<210>  4378
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4378
augaagaaaa ucgugaacua caaucugggc cucgacauug ggaccagcuc caucggauau       60

gccgcuauug augaccaggg cagacccguc cggcaccaaa acaagacagu uaugggggug      120

cgccuguuca aagagggaaa gacggccgaa ucuaccagaa ucuuucgggg cacacgcaga      180

cggcuuagcc aucgccacug gagacugggg uaccucgauc ggauuuucga caaugagauc      240

gcgaaagugg augaaaacuu ucugccucgc cuuaaggcuu ccaagagagc ccggucugac      300

gagaaacgca gcuucagagg aucccugcuc uuuccggaaa ccggcgauga cgccuauuac      360

cagaaguauc ccacaaucua ccaucugcgg uaugcucuga uuacccagaa aaagcaguuc      420

gauauccgcc ucauuuaccu ggcccuucac cauaugauca aauacagagg caauuuucug      480

gacacaaccc cugucucugc uuucaacauu cacgauaucg accucacgga ucaauuugcc      540

agccugaaug acugcuauca gcuucuggac auggauuucg aguuucaguu cgaccucaac      600

aauauugaac aggugcaaaa gcugcugcuc gaugaaaccc uguccaagac aaaacggcag      660

gcggccguua gcaaggugcu ugucaugucu accgggaaca aagccuuuga cgaucagcag      720

aauggaaucg ccuccgaguu caucaaggcu cugcucggcg ggcccuucga ccugagcaaa      780

auucuuaacc ugucuguggc cgauguuaag uccuggaaac uccacaugac agacaaggau      840

guggacgaua cccugaauag ccuggucgag gagcucgacg agaaccauaa gcaaaucgug      900

aaucugcuuc agaugauuua cucucaggug augaugcugg gacuccugaa cggccaacag      960

uccaucagcg cguccaugau ugaaagauau gacgaucacg ccaaacauug ggcugcccuu     1020

aagaaacugc ucccugcucu gggggagcag gaacagucua cgaucaucca cgccuacaca     1080

gccuauaccg gacacagccg cacaaagcaa cugucccggg cugaguuuua caccaaaguc     1140

aacagcauuc ucaagaagca ugaaaauauc gcggaggcca ccggccugau ugagcuuauc     1200

gacagaaacg aguucaugcc caaacagcgc gaugcuguua auggcgugau uccucaccag     1260

cugcaucagc gggaacucca acugaucauc cagaaccagc agaaguauua cccguuucug     1320

gccgccaagg cuuaccccga cgccgaugaa acaaugauug agcaacuucu cucuuucaga     1380

gugccuuauu acgcggggcc ccuggucgac cgcgauuuua ucaaugaauc cagcgaccag     1440

gagcaggcua ugcagaacac gaaguucucu uggcugaaac ggagagaggc cggagaaauu     1500

accccuugga auuuugagca aaagguggau guucaggaaa cagccaaccg cuucaucaag     1560

cggcucacca caaaagacac cuaucugaug acggaggaug ugcuucccaa ggaaagccug     1620

auuuaccaga gauuugaggu ccucaaugag cugaacgcua uccgcauuaa ugaccggaga     1680

cugacagccg aaaacaaaca acuugcuuuc cagcagcucu uuauggccgg caauaccaag     1740

aacgugucca ucaaaaaguu ccggaauuuu guuuucgauu cucugaaguu cccggagaaa     1800

gugaagauca ccggguuugc gggacagcac aacuuccugg ccaaacucag cacauaucau     1860

gacuuuaaga aaauuuucgg cgaacgcguc gaccacaccg aucugcaacc ugacuuugag     1920

agaaucgugg agugguguac aauuuucgaa gaucggcaca ucuacagaga gaaacuuaaa     1980

gaaauucagu ggcuguccca gagcgagauc gaccagcucg uccugauccg caaccauggg     2040

uggggacggc ugucuaagag acuucucacg ggcauucgcg auuccgacgg cagaaccgug     2100

auggaucaac ugugguuugg gacagacaau uucaugcgga ucauuagcca gucugauuuu     2160

gcugccgcua ucgccaacga aaaucgcacc cagcugaacc agcacggacu cgaggacguu     2220

cuggauuccg cguucaugag cccccaaaau aagaaagcca uuagacaggu gcuuaagguc     2280

guggacgaua ucgagcagac acugggcugc ccucccaaaa agguuaugau cgaauuuacc     2340

cggucccccg acaaaaagaa gcaacgcacg gugaccagac ucgagaaagu ccagcaggcu     2400

uauaaggccg uggcugacca gcuggccggg caaucuauug ugagcgaacu ggaucaguac     2460

gccaacacac auuccaaacu uucugacacc uacuaucucu acuucaugca gcugggacgg     2520

gaucuguaua gcggcaagcc uaucgacauu gauuccccga ugcucgacga ugaccacauc     2580

cugccccgcg cuuuuaugaa agauaauagc cuugacaaca gagcgcugac agagaagccu     2640

auuaauaaca ccaaggccgc uaaauucccc ucuaagguuu ucgggcagca aaaagaaguc     2700

acggauuuuu ggaagcuccu gcggcgcugu ggccugaugu ccaauaucaa agagaagcac     2760

cuuuaccugg acccugacaa cguggauaag uggacaagac ggggauucau ccauagacag     2820

cucguggaga ccagccaggu cauuaaaauc guggccaaua uucugaacga ccgcuaucag     2880

caaucucaga cagaaguucu cgaggugccc gcccggcugg gcaccgaacu uagagagugc     2940

uuuggguggu acaagguccg cgaagugaau gauuaucacc auguuuucga cgcuuaccug     3000

gccgcguuua ucggaaacua ccucuaucgg agauacccga aacugcagcc uuauuucgug     3060

uacggcaacu uugcugagcu gaauaaggcc cagcuuaaaa acauuaaguc cuucaauuuu     3120

cuguauaaca ucaccgauaa uaaacgcguc gugaaggaca uugagacagg gcaaacgcuc     3180

ugggaugacc agauccggca gcaaauccag cacauuuacg aauauaagua caugcugacc     3240

agccaugagg ccuucacaca gcacaaagaa cucuacggac agaccaucua ucccagacgc     3300

gaguuuaagc cuggcacacu gaaagcuauu aagcaagauc agccccagga gcuuuacggg     3360

ggcuauaaaa accugaccgc cgcguacaug uguaucguua agauucaggc ucggaagaga     3420

cgggccgccg aauaucgcgu ggucagagug gcuacggagu cucucagcgc ccugcaacag     3480

gcuaaacggc ugggagugca ggccuacgaa cgcugccuua gacaggagcu gguccggaau     3540

aacgaaaucc ucagaacaaa ugcggacccu aacuccgcuc cccgcgaguu cgagguucug     3600

cucgaucggg ugcuguguca ccaaaugauc auuagcgacg gccagaagua ucuucugggg     3660

ucucaugcuu acaaaaucaa uugcaugcag cuggugcucu ccaacaauag caugaagauu     3720

cuguccaccu cuaucgucaa acuuaagcaa augaccaacg aaccgcuggu gcagguuuac     3780

agagagauuc ucgauguggu caauaaguau uucacacugu acgacgugaa cagcuuucgc     3840

gaaaaccuca aucaguucuu ugacaaauuc cggacccugc cuaucacaaa cgaguaugaa     3900

uccucuaaga gcggaaagaa acuuauccac agaggccagc uggagacgcu gacccucauu     3960

cugcgcgggc uucaugccaa uuccgauaaa guucaauacg gagaccaguc uacacugcuc     4020

ggcggguuuc agaccaagga uggcaucaag cugagcaaag acuccgugau ucaguauaca     4080

agccccgcug gacucaacgu ccggaccgug aagcugagag aucuugagca aaaagaagcc     4140

cagaaguga                                                             4149


<210>  4379
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4379
augguggacg agaagaucuu cuggggccug gauauuggga ccgacagcgu cggaugggcc       60

guuacaaacu ccgaauacaa acucaagaaa uauaagaaua accugaugug gggcgugcac      120

cuuuuugaug aggcuaaaca gucugccgaa agacggagcu uccgcacggc gagacggcgc      180

cuggacagac ggaagcaacg caucauucuc cugcaggagu uuuucgugag agcugucugc      240

gaaaaggaug agaacuuuuu ccggcgccuu aaagaguccg cccugcuccc cgaagacgcc      300

gagcauagaa ccaacaauau cuuuuucgau gacccugauu acacagacaa ggauuauuuu      360

gaagaguacc cgaccauuca ccaucugauc ugugagcuga uggaaucuaa agagccccac      420

gacgugcggc ucguuuaucu ggcuugcgug uaccuucugg cccaucgcgg gcacuuccuc      480

cugccuguca gcgaagauga caucuccaag gugacagagu uugaaccccu uuaugagucu      540

uucuacaaag cucuggagga aaagcucgac gaugagccuc ccuuugacag aagcgccgau      600

gacuucgcgg aaauucugaa gucccacaaa accguuagcg ccaagaacaa agauuucgac      660

aagcugcucu uuggaggcaa agugaagaca uacgauaaug agaacaucuc uuauuccgcu      720

cugauuaaac uucugagcgg gggaaccgaa aagcucucua aguucuuugc caaugaggag      780

uacacggacc uggaaaaaga uuccgucugu gugcggaacg cugacuucgg cgauacccuu      840

gagaugcugg aaggccagau cgacgagcuc gacuuugccc ugcucaagag cgugaaaucu      900

cucuaugauu ggucccugcu ugucgacauu cuggaaggca agagccucau cuccgaggcg      960

aaaaaggaua aguacgacga gcauggauau gaucuggacg cccuuaaaua ccuguuccgc     1020

gaauaucuca caaaggauga cuacaaugag auguuuaaag aaguuucugg caagcagaac     1080

uacgcuagcu auguguacaa ugccuccagc gauaaaacca gagacucuaa guauaagaaa     1140

ugcucccaaa aggauuucug uaaauuuaca aagaaauucc ugagcaagau uaagccuaac     1200

gagaaagaca agccgugccu ggauaaacuc cuggagaagu gugaacaaaa uucucuuugc     1260

cccaaacagg ugaccacgga caaccggguc aucccuuacc agcuguauua cguggagcuc     1320

aagaaaaucc uggaaaacgc uuguggguau cugcccuuuc uuaaugagag agacgaauac     1380

ggaacaguug ccgauaagau ucugagcauc augaaguuca gagugccuua cuaugucggg     1440

ccccuggugg acuccaaaaa gucuccgaau gccuggcugg uucggaaacu cgaugggaag     1500

auuaccccuu ggaauuuuac agacauggug aacgaggaug agagcgaaaa ugcuuucauc     1560

agacgcauga ccugcaaaug uaccuacguc gcgggacagg acgugcugcc caaguauucc     1620

cuucuguaca gcaaguucuc uguccucaac gagauuaaua acaucaaacu gaauggcgaa     1680

ccuaucuccg ugcagacaaa gcaggagauu uauacggaac uguuugagcg gaacaaaagc     1740

agaguuucua agaaaaagau ccgcgauugc cuuauuuccc acggcuacgc cgcugacagc     1800

gaugagguga ccgggaucga cgauauugcc aaguccgccc uccggucuua ucaugacuuc     1860

aaaaagaugc ugagcaaugg aauccugaca gaacagcaag ucgaggaaau cauugagcac     1920

aucaccguga caaccgauaa cauuagacuc aaaaaguggc ugaaaacgca guuucccaug     1980

cuugcugacg aagacguuaa guacaucaca aagcugaaau acaaggauua uggccgccuc     2040

ucccggugcu uccuggagga cgugcugccc gucgauacca aaaccgggga ggccgaaucu     2100

gacaagaaua uuaucacaau gcuuugggag accaacgaaa auauuaugca gcuccugagc     2160

uccaaauaca gauauagcga gaacaucgag cacaugaacc gccaauacua ugcgcugccu     2220

gaaaaucaua agucuauguc cgagcggcuc aaagauaugu acgugccgac agcugugaga     2280

cgggccguua cgcgcaccuu ugacaucguc aaggaacuga agaaaauuca gggaagagau     2340

cccgacaaga ucuucauuga gauggcccgg ggcacagggg aaaccccuaa aggaaagaga     2400

acaaacagcc gcaaagauca gauccuugag cacuggcaug gccuggacaa uaaggauauu     2460

aacgaccuca agaaaucugg caucugggag caccuggaca ccaucgauga cgcuaagcug     2520

cgguccgaua aauauuuucu uuacuucaug cagcucggga gauguaugua cacggaaaag     2580

cccauuccuu uugaggaagu ggagaaugaa cauaaaugga acaucgacca cauuuggccc     2640

caagccaaga ucaaggauga cagccuggau aauaaagugc uggucucuag caacgagaau     2700

ggaaagaaau ccgacagcua uccgauuucu gaugacaucc gccacuccau ggcuggccuc     2760

uggcauagcc uguacaagaa agggcuuauc uccgagaaga aguaucagag acugacccgg     2820

ucuacaccuu ucaccgauga cgaacucagc ggauuuauug cccgccagcu gguggagaca     2880

agacagucca ccaaagcggu ugccacgcug cuuaaggaac aauuccccaa cacagagauc     2940

guguacguca aagcuggccu ggugucugau uuucggcagg aaauggggau gcucaagugc     3000

cgcgagguua augaccugca ccaugccaaa gacgcuuauc ucaacauugu gcugggcaau     3060

gucuacaaca ccagauucac aaaggauccu cuuaauuucg ugaagaacaa cgagaaauau     3120

agcaucaaga uuuuucagaa aaauuccgac ggaaagaaaa ccggcguuau cacccggaag     3180

guggaacgcg ggggagaggu cgccugggau cccgaaacau cuuucgcgau cgugagaaaa     3240

augaugagca agaacuccau ucgguacgug cgcuacgccu auaugagaaa gagcggccag     3300

aaugggggau uuuucgacca aaacccucug cggaaaggca aggggauggu cucuaucaaa     3360

cacaaucucu ccauugagaa guacggcgga uaugauaacg cuacgaucag cuuuuucucu     3420

cugguuucca uuaaagacuu uggcgugauc cugcuugccg uggagcugcu cagcgcuaau     3480

aaguuccuga agaacauuuc ugaagccaaa gaguacgccg cugauaagcu cuccaaagaa     3540

cugaauguca agaucgugcc caacgacguu gcguuuccgc uuaauaacag auauaaugug     3600

gucaaaauca acgccgugcu ggagcucgau ggguaccgcg uuaugauugc ugccaagcag     3660

aauagcucca ucaaguucgu gagcaacgaa ucucugauuc ugagcuccca gcacauggcc     3720

uaugucaaaa agaucaccaa auuuauugag aagcggaaac aggacaaggu guguaugauc     3780

aacgcuuucu cuggaaucag cauugaggau aauaucgaac uuuacgacgu ucuggugucc     3840

aagugcaaca uuaaaccuuu caauaucaug uacgagaacc ucggcauuaa gcugucugau     3900

aauagagaca acuuuaucaa aaagagcguc gaaaaucaag ugcucauccu guccgagaug     3960

gugcgccuuu ucaaaacagg gcggaaggcc ggagucaacc uggaagaggu uggcgggcgc     4020

aaaaauucug gcgugaagac ccugaacaca ugucucuaua aggugaaaga cuacagcaau     4080

auuuuucugg ucgaucaguc ccccaccgga cuuaucgagc auaagagccc caaccugcuc     4140

gaccuguga                                                             4149


<210>  4380
<211>  4155
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4380
augaacaagc ccuacagcau cggccuggac auugggacca auuccguggg auggucuauc       60

auuacagaug acuauaaagu cccugccaag aaaaugagag uucucggcaa cacggauaag      120

gaguacauca aaaagaaucu gauuggggcc cuucuguucg acggaggcaa caccgccgcg      180

gaucggcgcc ucaagagaac agcucggcgc agauauaccc ggcgcagaaa ucggauccug      240

uaccuucagg aaaucuuugc cgaggaaaug agcaaagugg acgauuccuu cuuucaccgc      300

cuggaggacu cuuuccucgu ggaagaggau aagagaggga gcaaauaucc gauuuuugcc      360

acacugcaag aggaaaagga cuaccaugag aaauucucca ccaucuauca ucugcggaag      420

gaacucgcug auaagaaaga gaaggccgac cugcgccuua uuuacaucgc ucuggcccac      480

auuaucaaau uuagaggaca cuuccucauu gaggaugacu cuuuugacgu ccggaacaca      540

gauaucagca agcaguacca ggacuuccug gaaaucuuua auaccacguu cgagaacaau      600

gaucuucugu cccagaacgu ggacguugaa gcgauucuca ccgauaaaau cagcaagucu      660

gccaaaaagg accgcauucu ggcucaauau cccaaucaga aguccacagg caucuuugcc      720

gaguuccuga aacucauugu gggcaaccag gcugauuuca agaaauacuu uaaucuggaa      780

gacaagaccc cucuucaguu cgccaaagau agcuaugacg aggaucugga gaaccuccug      840

gggcaaaucg gagacgaauu ugcggaccuu uucucugccg cuaagaagcu guacgauucc      900

guccuccuga gcggcauccu gacagugauu gaccucucua ccaaagcucc ccuguccgcu      960

agcaugaucc agagauauga ugagcaucgg gaagaccuua agcagcugaa acaguuuguu     1020

aaggccuccc ucccugagaa auaccaagaa auuuucgccg auucuagcaa ggacggguau     1080

gcuggauaca ucgagggcaa gacgaaucag gaggcguuuu acaaauaucu guccaaacuu     1140

cugacaaagc aggaagauag cgagaacuuc cucgaaaaga uuaaaaacga ggacuuucug     1200

agaaagcaac gcaccuucga uaaugggucu aucccccacc aggugcaccu gacagaacuc     1260

aaggccauca uucggagaca guccgaguac uauccuuuuc ugaaagagaa ccaggaccgc     1320

aucgaaaaga uucuuaccuu ccggaucccg uacuauauug gaccccuggc uagagagaaa     1380

agcgauuuug ccuggaugac ccgcaagaca gacgauucua uccggccuug gaauuucgaa     1440

gaccucgucg acaaagaaaa gagcgccgag gcuuucauuc auagaaugac gaacaaugau     1500

uuuuaccugc ccgaggagaa agugcugccu aagcacuccc uuaucuauga aaaguucacc     1560

guguacaacg agcucacaaa aguccgcuac aagaaugaac aaggcgagac cuacuuuuuc     1620

gacucuaaca ucaaacagga gauuuuugau ggcguuuuca aggaacaucg gaaagugagc     1680

aagaagaaac ugcuggacuu ucucgccaag gaguaugaag aguucagaau cguggauguc     1740

auugggcugg acaaagaaaa uaaggcguuu aacgcuuccc uuggaacaua ucacgaucug     1800

gagaaaaucc ucgacaagga uuuccuggac aaucccgaua acgagagcau ucuggaagac     1860

aucgugcaga cccuuacgcu cuuugaggau cgcgaaauga ucaagaaacg gcuggagaau     1920

uacaaggacc uguucacaga aucucagcuc aaaaagcugu auagacggca cuacaccggc     1980

ugggggcgcc uuuccgccaa acugauuaac ggaaucagag acaaggagag ccaaaagacc     2040

auucucgauu aucugaucga cgauggccgg ucuaauagaa acuuuaugca gcugauuaac     2100

gacgaugggc gcuccaaucg gaacuucaug cagcuuauca augacgaugg acucagcuuc     2160

aaauccauca uuucuaaggc ccaggcuggc agccauuccg acaaccugaa agagguugug     2220

ggcgaacugg ccgggucucc ggcuaucaag aaaggcauuc uccaaagccu gaagaucguc     2280

gaugagcuug ugaaaguuau gggauacgaa ccugagcaga uuguggucga gauggccaga     2340

gaaaaucaga caaccaacca ggggcgcaga aauucccggc aacgcuacaa gcugcucgac     2400

gauggaguga agaaccuggc gagcgaccug aauggcaaca uccuuaaaga guaucccaca     2460

gacaaucagg cccuccagaa cgaaagacug uuucuguacu aucuccaaaa ugggcgggau     2520

auguacacgg gcgaggcucu ggacaucgau aaccuuucuc aguaugacau ugaucacauc     2580

auuccucagg ccuucaucaa ggacgauucc auugacaauc gcguccuggu gagcucugcu     2640

aaaaacagag gaaaguccga ugacguuccc agccucgaaa ucgugaaaga uugcaagguc     2700

uuuuggaaga aacugcugga cgccaaacuu augucucagc ggaaguacga uaaccugacc     2760

aaggcggagc gcggcgggcu cacaagcgac gacaaagcca gauucauuca acggcagcug     2820

guggagaccc gccagaucac aaagcauguu gcuagaaucc ucgaugaacg guuuaauaac     2880

gagcuggacu ccaagggaag acgcauucgg aaagugaaga ucgucacccu uaaaagcaau     2940

cuggugucua acuucagaaa ggaauuuggc uucuauaaaa uucgcgaggu gaauaacuac     3000

caccaugccc acgaugcuua ccucaaugcc guugucgcca aggcuauccu gacgaaguau     3060

ccccagcugg aaccugaguu uguguacggg gacuauccga aauacaacuc cuauaagacc     3120

cggaaaagcg cgacagagaa acuuuucuuu uacuccaaua uuaugaacuu cuucaagacc     3180

aaggugacac uggccgaugg aaccgucgug guuaaggacg auaucgaagu gaauaacgac     3240

acgggcgaga ucgucuggga uaaaaagaaa cacuuugcua cagugagaaa gguucucucu     3300

uauccccaaa auaacauugu gaaaaagacc gaaauccaga cagggggcuu cagcaaagag     3360

uccauucugg cccauggaaa uucugacaag cucaucccuc gcaagaccaa agauauuuac     3420

cuggacccca agaaauacgg cggguuugau agcccuaucg ucgccuauuc cgugcuuguu     3480

guggcugaca ucaagaaagg aaaggcccag aagcugaaaa ccgucacaga acuccugggc     3540

auuacgauca uggagcgguc uagauucgag aagaacccca gcgcguuucu ggaauccaaa     3600

ggguaccuua acauucgcgc ugacaagcug aucauucucc cgaaauauag ccuguucgag     3660

cucgaaaaug gacggagacg gcugcuugcc ucugccggcg agcugcagaa ggggaacgag     3720

cuggcucucc cuacccaauu uaugaaguuc cuguaccuug ccucccgcua uaaugaaagc     3780

aaaggcaagc ccgaggaaau cgagaaaaag caggaauuug ugaaccagca cgugucuuac     3840

uucgaugaca uucugcaacu caucaaugau uuuuccaaaa gagucauccu ggcggacgcu     3900

aaccucgaga agauuaauaa gcuguaucag gauaacaaag agaauaucag cguugacgaa     3960

cuugccaaca auauuaucaa ccuguucaca uuuaccucuc ugggagcccc ugcugccuuc     4020

aaguucuuug auaaaauugu ggaccggaag cgcuacacau ccaccaaaga ggugcucaau     4080

agcacgcuga uccaucaguc caucacaggc cuuuacgaaa ccagaauuga ucuggggaag     4140

cucggagagg acuga                                                      4155


<210>  4381
<211>  4155
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4381
augaagcuga gaggcaucga ggacgauuac agcauugggc ucgacauggg aaccuccucu       60

gugggcuggg ccgucacaga ugaacggggg acgcuggcuc acuucaaacg caagcccacc      120

uggggaagca gacuuuuucg ggaggcccag acagcggcug uugcccgcau gccuagaggc      180

caacggcgca gauaugugcg gcgcagaugg cggcuggacc uccugcagaa acuuuucgaa      240

cagcagaugg agcaagccga uccggacuuu uucauccgcc ugagacaguc ccggcuccug      300

cgcgaugaca gagcugaaga acaugccgau uaccgguggc cccuguuuaa cgacugcaag      360

uucaccgaga gagauuauua ccagcgcuuu ccuacaauuu aucacgugcg gucuuggcuc      420

auggaaaccg acgagcaggc ugauaucaga cugauuuacc uugcccugca uaauaucguc      480

aaacaccgcg ggaacuuccu ccgggaagga caaagccugu ccgcgaaguc ugccagaccc      540

gacgaggcuc uuaaucaucu gcgcgagaca cuccgggugu ggagcuccga aagaggcuuu      600

gaguguagca ucgccgacaa cggcucuauu cuggcuaugc ugacccaccc ugaucucucc      660

cccagcgacc gccggaagaa aaucgccccu cuguucgaug uuaagucuga cgaugcggcc      720

gcugacaaaa agcuggggau ugcccuugcu ggagccguga ucggccucaa aacggaauuu      780

aagaauauuu ucggggauuu uccgugcgag gacuccagca ucuaucuguc uaacgaugaa      840

gccgucgacg cugugagauc cgcguguccc gaugacugcg ccgagcuuuu cgaccgccug      900

ugugagguuu acagcgcuua cgugcuccag ggacugcugu ccuaugcccc uggccagacc      960

auuucugcca auauggucga aaaguaccgg agauaugggg aggaucucgc ucugcuuaaa     1020

aagcugguga aaaucuacgc ccccgaccag uaucggaugu ucuuuagcgg agcgacauac     1080

ccuggcaccg gcaucuauga ugcugcccaa gcccgcgggu acacaaagua caaccucgga     1140

cccaaaaagu ccgaauauaa accgagcgag ucuaugcagu acgacgauuu cagaaaggcu     1200

guggaaaagc uguuugccaa aaccgacgcu cgggccgaug agagauaucg caugaugaug     1260

gaccgguucg auaagcagca auuucuuaga cgccugaaaa cguccgacaa uggcagcauu     1320

uaccaccagc uccaucugga ggaacugaag gcgaucgucg agaaccaggg gagauucuau     1380

ccuuuucuca aacgggaugc cgacaagcug guuucucuug ugagcuuccg cauucccuac     1440

uaugugggac cucuguccac aagaaaugcu cggaccgauc agcacggcga aaaccgcuuu     1500

gccuggucug agagaaagcc cgggaugcaa gacgaaccca ucuucccuug gaauugggag     1560

agcauuaucg accgguccaa aagcgcugag aaguuuaucc uccgcaugac aggaaugugc     1620

accuaccugc agcaggaacc gguccugccc aaaucuuccc uucucuacga ggaauuuugu     1680

gugcugaacg agcugaaugg cgcccauugg agcauugaug gcgacgauga gcacagauuu     1740

gacgcggccg aucgggaagg gaucauugag gaacucuucc gcagaaagcg gaccguuucu     1800

uauggagacg uggcuggcug gauggagaga gaacgcaacc agaucggggc ccacgucugc     1860

ggaggccaag gggagaaagg cuucgagucc aagcugggaa gcuacauuuu cuucuguaag     1920

gauguguuua aaguugaacg gcuugagcag uccgacuauc cuaugaucga aagaaucauu     1980

cuguggaaca cacucuucga ggaucgcaag auccugucuc agcggcugaa agaagaguac     2040

ggcagcagac uuuccgcuga gcagauuaag acgaucugca aaaagcgcuu uaccgggugg     2100

ggacggcucu cugaaaaguu ccugacaggc auuaccgugc aagucgacga ggauagcgug     2160

uccaucaugg acguccugag agaagggugu cccgugagcg gaaaacgcgg ccgggccaug     2220

guuaugaugg agauucucag agaugaggag cugggguuuc agaagaaagu ggacgacuuc     2280

aaucgggccu uuuucgcuga gaacgcgcag gcccuuggcg ucaaugaacu gccuggaucu     2340

cccgcugugc gcagaucccu caaccagagc auccggaucg uugaugagau ugccucuauc     2400

gccggcaagg cuccggccaa uauuuuuauc gaagugacac gcgacgagga uccuaaaaag     2460

aaagggagac ggaccaagag acgcuauaac gaccugaagg augcgcugga ggcuuucaaa     2520

aaggaagacc ccgagcuuug gcgggaacuc ugcgagacgg ccccuaauga uauggacgaa     2580

agacuguccc uguacuuuau gcaacgcgga aaaugccucu auagcggccg ggccauugau     2640

auccaucagc ugucuaacgc ugggaucuac gaggucgacc acauuauccc cagaacauac     2700

gugaaggaug acagccuuga gaauaaagcc cugguguauc gcgaagagaa ccagcggaag     2760

accgacaugc uccugauuga uccugaaauc agacgcagaa uguccggaua cuggcggaug     2820

cugcaugagg cgaaacuuau uggcgacaag aaguuccgca aucuccugag aagccggauc     2880

gaugacaaag cucugaaggg guucaucgcc cgccaacucg uugaaaccgg ccagaugguc     2940

aaacugguga gaucucuucu ggaggcccgg uaucccgaga caaacauuau cuccgugaag     3000

gcuagcauuu cccacgaucu cagaaccgcc gcugaacugg ucaagugucg cgaggccaau     3060

gacuuucauc acgcgcacga ugccuuccug gcuugccggg ugggacuuuu uauccagaaa     3120

agacauccgu guguuuacga aaacccuauu ggccugucuc aggugguccg caauuaugug     3180

cggcaacagg ccgacaucuu caagagaugc cggacaaucc ccgggagcuc cggauuuauu     3240

guuaacucuu ucaugacgag cggcuuugau aaagagaccg gggaaaucuu caaggacgau     3300

ugggacgcug aggccgaggu ggaaggcauu cgcagauccc ucaacuuucg gcaguguuuc     3360

aucucucgca ugcccuuuga ggaucacgga gucuucuggg acgccacaau uuacagcccu     3420

agagcuaaaa agaccgcggc ccugccgcuc aagcaggggc ugaaucccuc ccgguauggc     3480

agcuucucuc gcgaacaauu ugcuuacuuc uuuaucuaca aagccagaaa cccucggaag     3540

gagcagacac uuuucgaguu ugcccaggug cccguucgcc uguccgcuca gauuagacaa     3600

gacgaaaaug cccucgagcg guaugcgaga gaacuggcua aagaucaggg acuggaguuc     3660

auccgcaucg aacggagcaa gauucuuaaa aaccagcuga ucgagauuga cggcgauaga     3720

cucugcauca ccgggaagga ggaagugcgc aaugccugug agcuggccuu ugcucaagac     3780

gaaaugcggg ucauuagaau gcucgugucu gagaaaccug ugucccgcga augcgucauc     3840

agccuguuca accggauccu ucugcaugga gaucaggccu cuagacgccu cuccaagcag     3900

cugaagcugg cgcuucugag cgaggcuuuu uccgaggcca gcgacaaugu ucagcggaac     3960

guggugcucg gccugauugc caucuucaau gggucuacga acauggucaa ucucagcgau     4020

auuggaggcu ccaaauuugc uggcaacgug agaaucaagu acaaaaagga acuggccucu     4080

cccaaaguua augugcaccu uauugaccaa agcgucaccg ggauguucga gcgccggaca     4140

aagaucggac uguga                                                      4155


<210>  4382
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4382
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

aucacggaug acuauaaagu uccuucuaag aaaaugaagg ugcucggcaa uaccagcaaa      120

aaguacauua agaaaaaccu gcuuggggcu cuccuguucg auuccggaau cacagccgag      180

ggcagacggc ugaagcgcac cgcgagacgg cgcuauacaa gacggcgcaa ucggauucuu      240

uaccugcagg aaaucuuuuc uaccgagaug gcuacacucg acgaugccuu cuuucaaaga      300

cuggacgaua gcuuccuggu gccggacgau aaacgcgacu ccaaguaucc caucuuuggg      360

aaccucgucg aagagaaagu guaccacgau gaguucccua ccauuuauca ucugagaaag      420

caccuugccg acucuagcaa gaaagcugau cugcggcucg uuuaccuggc ccuugcucau      480

augaucaagu accgcggaca cuuucugauu gaaggcgacu ucaauuccaa aaacaaugac      540

cuccagaaga acuuucagga uuuccuggac acguauaaug ccaucuuuga gagcgaucug      600

ucucucgaaa acuccaaaca gcuggaggaa auugugaagg acaaaaucag caagucugcg      660

aagaaagauc ggauucuuaa gcuguucccc ggcgagaaaa auuccgggau cuuuagcgag      720

uuccucaagc ugaucgucgg aaaccaagcc gacuucagaa aauacuuuaa ucuugaugaa      780

aagaccucuc ugcaguucuc caaggagagc uaugacgaag aucucgagac acugcugggc      840

cauauugggg acgauuacuc cgacguguuu cucaaagcua agaaacugua ugacgccauc      900

cuucugucug gcauucucac cguuacagau aacggaaccg aagcuccucu gagcuccgcc      960

augaucaugc gcuacaagga gcacgaggaa gaccuugcgc ugcucaaagc cuauauuaga     1020

aauaucagcc uggagacgua caacgaagug uucaaggaua acacaaagaa uggguacgcu     1080

ggauauaucg acggcaaaac cucucaggag gauuuuuacg ucuaucugaa gcggcuccug     1140

gccggccuug aaggggcuga cuacuuccug gagaaaauug auagagagga cuuucuccgc     1200

aagcagcgga cauucgauaa cggauccauc cccuaucaaa uuuaccugca ggaaaugaga     1260

gccauccugg acaaacaggc caaguuuuau ccuuuccuug cuaagaauaa agagcgcauu     1320

gaaaagaucc ucaccuuucg gaucccguac uacgugggcc cccuggcgag agggaacagc     1380

gauuucgccu ggucuauucg caaacggaau gagaagauca ccccuuggaa cuuugaagac     1440

gugauugaua aagagagcuc cgcugaggcc uucaucaaua gaaugacauc uuucgaccug     1500

uaucuccccg aagagaaggu ccugccuaaa cacagccuuc uguacgaaaa cuuuacgguu     1560

uauaaugagc ucaccaaggu gcgcuucauu gccgagggaa ugcgggacua ccaguuucug     1620

gauuccaagc aaaagaagga caucgugaga cuguauuuca aaggcaagcg caaagucaca     1680

gauaaggaca uuaucgaaua ccuucaugcu aucgaugggu augacggaau ugagcucaag     1740

ggcaucgaaa aacaguuuaa cagcucucug uccaccuacc acgaucugcu caauauuauc     1800

aacgacaagg aguuccugga ugacagcucu aaugaaacaa uuaucgagga gaucauucau     1860

acccuuacga uguuugaaga ucgggagaug aucaaacaga gacuguccaa guucgacaac     1920

auuuuugaua aaagcgugcu caagaagcug ucccggcgcc acuacacagg cugggggaaa     1980

cugucugcca aacuuaucaa uggaauuaga gacgaaaaga gcggcaacac cauccucgac     2040

uaucugaucg augacggggu uuccaaccgg aauuucaugc aacugauuca cgaugacgcg     2100

cucucuuuua agaaaaagau caagaaagcu cagauuaucg gagauaagga caacauuaaa     2160

caggugguca agagccugcc cggcuccccg gccaucaaaa aggggauccu ucagagcauu     2220

aaaaucgugg augagcuggu uaaggugaug ggcagagaac cugagucuau ugucguggag     2280

auggcccgcg aaaaucaaua caccaaccag ggaaagucca auagccagca gcggcucaaa     2340

agacuggagg aaucucugaa gggccuuggg uccaaaaucc ucaaggagaa cguccccaca     2400

cgccugagca aaauugacaa uaacgcucug caaaaugaua gacucuaucu guacuaucuu     2460

cagaacggaa aggacaugua caccggcgag gaacuggaua ucgaccggcu cucuaauuau     2520

gacauugauc auaucauccc ucaggccuuc cugaaggacg auagcauuga caacaaagug     2580

cugguuucca gcgcuucuaa ucgcgggaag uccgaugacg ugcccagccu ugaggucgug     2640

aaaaagagaa aaacacugug guaccagcuc cugaagucca agcucaucuc ucaacggaaa     2700

uuugauaacc ugacgaaggc cgaacgcgga ggccuuagcc aggaggaaaa agcgggguuc     2760

auucagagac agcugguuga gacccggcaa aucacaaagc acguggcccg ccuccuggac     2820

gagagauuca auaacaaaaa ggaugaaaac aaucggaccc ugcgcacagu caagauuauc     2880

acccuuaaau ccucucuggu gagccaguuu agaaaggacu ucgagcucua caaagugcgg     2940

gaaaucaacg auuuucauca cgcucaugac gccuaucuga augcuguugu cgccuccgcg     3000

cuccugaaga aauaccccaa acuugagccu gaguucgugu auggcgauua cccgaaguau     3060

aacucuuuua gagaacgcaa aagcgccacg gagaaggugu acuucuauuc caauauuaug     3120

aacaucuuua aaaagagcau uccccuggcu gacggaaccg ucaucgaccg gccucucauu     3180

gaagugaaug aggaaacagg cgagucuguu uggaacaaag uggccgaucu gaauaccguc     3240

agaaaggugc uguccuacag ccagguuaac aucgugaaaa aggucgagga acaaaaucac     3300

gggcuugacc gcggaaagcc caaaggccug uucaacgcua aucucucuuc caagccuaaa     3360

cccaacagca aggagaaucu ggugggggcc aaagaauacc ucgauccgaa gaaguaugga     3420

ggcuacgccg ggaucucuaa cuccuuugcu auucugguua aaggcacaau cgagaaggga     3480

gcgaaaaaga aaauuaccaa cgugcuugag uuccagggca ucagcauucu ggaccggauc     3540

uauuacagaa aggauaagcu gaauuuucuc cuggaaaaag gguauaagga cauugaacuu     3600

aucaucgagc ugccuaaaua cucccucuuc gagcugagcg auggaucucg ccggaugcuc     3660

gccagcauuc uguccacgaa caauaagaga ggcgaaaucc acaaagggaa ccagauuuuc     3720

aucucucaga aguuugucaa acuucuguau caugcuaagc gcauuagcuc cacauucaau     3780

gagaaccacc ggaaguacgu ggaaaaucau aaaaaggagu uugaggaacu guucuacuau     3840

auccucgagu uuaacgaaaa uuacguggga gccaaaaaga acggcgagcu gcuuaaaucu     3900

gccuuccaaa gcuggcagaa ucacuccauc gacgaacugu gcucuagcuu uauugggccc     3960

accggcuccg agagaaaggg acucuucgag cugacaagcc ggggcggggc ugccgauuuu     4020

gaguuccucg gagucaagau cccucgcuau agagacuaca cccccucuuc ccugcuuaaa     4080

gaugcgaccc ugauucacca gagcguuaca ggccuguaug aaacgcggau cgaccucggg     4140

aagcugggag aagauuga                                                   4158


<210>  4383
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4383
augcagaccu acuucggcau gaagaaaauc cuggggcucg accugggaac aaacagcauu       60

ggcugggccc uugugaauga ggcuaacgau gacaaugaaa aguccucuau cauuaaacug      120

ggggucagag uuaacccccu cacgguggau gagcaaagca auuuugaaaa gggaaaaucc      180

aucaccacaa acgccgagcg gaccauuaag cgcucuauca gacggagccu gcagcgcuau      240

aaacuuagac gggaaaaucu gaucgagauu cucaaagagc acaacuucau cacagacgau      300

accauucugu ccgaagacgg caaugcgucu acauuugaga ccuaccgccu gagagcuaag      360

gccgccagcg aaaagaucuc ccucgaggag cuggggcggg ugcuucugau gauuaacaag      420

aaacgcggau auaagagcuc uagaaaggcu aaaaaucagg aagagggcca gcucaucgau      480

ggcauggaag ucgccaagga gcuguacgaa aagaacauua cgccugggca auucguguuu      540

cagcuucuga agcagggagg caaauucauc ccggacuuuu aucgguccga ucuccaggcu      600

gaguucgaca agaucuggga guuucaaaaa cuguuccaug ccgaaauucu gguugaugac      660

uuuaagaagc agcucgagcg caaagggaag cugaauagcu cuaaaaucuu ccuuggaaga      720

uuuggcauuu acaccgcgga aaacaagggg aaugauaaac ggcugcaggc cuaucagugg      780

cgcguggacg cucucacaaa ggagcugacc aaggaggaac uugccuacgu caucuccgac      840

cugaacggaa uuaucuccuc uuccagcggc uaccucggcu ccaucucuga uagaagcaaa      900

gagcuguauu ucaacaauca aacagugggg caguuccuga uggcucagcu cgaccacagc      960

ccccauacca gccugaagaa ccaaguguuu uaccggcagg auuaucuuga cgaguucaau     1020

acgcuguggg aaaaccaggc ccacuaccau aaaguucuca caucugaucu gaaguccgaa     1080

auuagagacg ucaucaucuu uuaucagcgc cggcuuaaaa cccaaaaggg acugauuagc     1140

uucugcgagu uugaaucuag aaagauugag guggagaucg auggcaaaaa gaaaaucaag     1200

aaugugggga gccgcgucug uccuaaaucc ucucccauuu uccaggaauu uaagaucugg     1260

cagauucuca acaauaucga gcugcuggaa aagagcucca aagcgaagcg guuccucgac     1320

caggaggaga aagaacugcu uuuuaaggag cugagcauua aaucuaagau cuccaaaagc     1380

gaugcccuca agcugcuguu caagaacucu agagaacuug accucaauua caaagagauu     1440

gaaggaaacc gcacacaauc cgcucuguuu gaugccuaua gcaagaucau cgagcugucc     1500

ggccacgggg aguacgacuu cucuaaaaug gaagcuagca auauuaucga gcucguucag     1560

gaaguguuua ccggacugaa cuacaauacc gagauucuuu auuucgacuc cucucuggaa     1620

ggcaaggauu ucgagaacca gccuauguac cggcucuggc aucugcugua uagcuuugag     1680

ggcgacgauu ccaaaacagg gaaugaaaaa cuuaucaaca agcucagcga gcuguacgga     1740

uucgaaaaga aguauucuau ugugcuggcc aacgucuccu uucaggagga cuacggcagc     1800

cucucuacga aagccaucag aaagauccac cccuaucuga aagaagggau ucaauacgau     1860

guggcuugcg aguucgcggg auaccgccac uccaagagcu cucuuaccaa ggaggaaauc     1920

gagaauaaaa uucugaagga ccagcucgaa auccugccua aaaacagccu gcggaauccg     1980

guuguggaga agauucuuaa ccagaugauc aaugucguga acgcccucaa ugaguccuau     2040

ggcaaacccg augaaaucag aauugagcug gcucgcgaac ugaaaaagag cgccaaagag     2100

cgggaggaac ucacagccuc uaucggggag accacaauua agcaugaaaa auacagagcu     2160

auccuggaga acgaauuugg cauuucccac guuagccgga augacaucau ccgcuauaaa     2220

cuuuaccugg agcucgaaaa cagaggauau aagacccugu acuccaauac guaugugucu     2280

caggagaagc uguucagcaa agaguuugau auugaccaca ucauuccuca auccugucuu     2340

uucgaugacu cuuuuagcaa caagacacuc gaauccaagu cugucaauau cgagaaaggc     2400

aacgauaccg ccuucgacua cguguuugcg aaguacgggg aagcuggacu gagcgacuau     2460

auuauccaua uugaggaacu guucaaagau ggcaagaucu ccaaaaccaa gcucaauaag     2520

cugaaaauga aggggaacga gauccccagc gacuuuauug cccgggauau cagagacaca     2580

caguacauug cccgcaucgc uaaaaacauu cuugaggaaa ccguccggaa ugugguuucu     2640

acauccggaa gcaucacgga uagacugcgc gaggacuggc agcucaucga ugugaugcaa     2700

gaacugaacu gggagaagua uucugcccug ggccuuaccg agauuuacga aaauagagag     2760

gggcacacaa uccggcgcau uaaagacugg accaagagaa acgaucaucg gcaccaugcu     2820

auggacgccc ugacaaucgc guucaccaag cgcucccaca uucaguaucu caauaaccug     2880

aaugccagaa gcgauaaauc uggcuccauc uacggaaucg aaacgaagga gcucuaucgg     2940

gacgaucacg gcaaacugaa guucaaaccu cccauuccgc uuaacgaauu ucgcgcugag     3000

gccaagaagc aucuggagaa uacccucauc agcauuaaag cuaagaacaa agucgccaca     3060

aucaauauua acaccacauc caccaaggaa gggaaaaaua agaagagaca gcugacgccu     3120

cggggacagc ugcaccuuga gacaaucuac ggcagcauuc aacaguacgu gaccaaacag     3180

gaaaagguug gggcgucuuu caacgaggaa cagaucagca aaguggccaa gcaaaaauau     3240

cgccaggcuc ugcucaauag acugcgggag uuugacaacg accccaagaa agccuucaca     3300

ggaaacaauu ccuuugagaa gaacccuauc uaccucgaug uccugcagac ccagaaggug     3360

cccgacaaag uggaaaccgu uucuaaggag auuaucuaua caauuagaaa agauaucacg     3420

cccgaacuua agauugagaa agucaucgac aagaagauca aaaccauucu gcaaagccgc     3480

cucaaugaau acuccggcga uucuaagcgg gcuuucagca accugaaaga gaauccuauc     3540

uggcugaacc aggagaaggg gauugaaauc aaaagaguga caauuuccgg caucucuaau     3600

gccguggcca uccaugacaa gaaggauaaa aacggaaauc uuauucugga caacgagggc     3660

agcacccagg cugucgauuu ugugaauaca gggaacaauc accacguugc gaucuauaag     3720

gacgaacgcg gaaaccucca ugaguccgug gucagcuucu uugaggccug cacccgggug     3780

aaucugggcg ggccgguuau uuucaaaaac uacaauaagu cugauggaug ggaauuucuc     3840

uucuccauga aacaaaacga guauuucgug uuucccaacg aaaagacggg cuucaauccu     3900

aacgagaucg accugcuuga ccccgauaau uacagacuga uuagcccuaa ccuguuucgc     3960

guccagaaga ucucuuccaa aaauuacgug uucaaccacc aucucgaaac aagcgcuauu     4020

accggggaga cccugaagaa uaaaaagcag cuuuccucug uuacauauca cuuuauccag     4080

agccugcccc cgaucaaaga cauugugaag guccggauca acaauauugg ccaaaucgug     4140

ugggugggag aguacuga                                                   4158


<210>  4384
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4384
auggacgagc ugcagaacuu cgaaaagggc aaaagcauca ccacaaaugc cgauagaacg       60

gcugggcggu ccaugcgcag aaaccuccaa cgguacaagc ugcgcagaga gaaucuuauu      120

gaagugcuga aagaguauaa cuuuaucacc gacgaaauga uucucucuga gaauggaaac      180

cggacaaccu ucgagacaua ccgccugaga gccaaggcgg cugucgaaga gaucagccuu      240

gaacaguuug cccggguucu gcucaugauu aauaaaaagc gcggcuauaa guccucuaga      300

aaagccaagg gggcugagaa cggaacccug aucgauggca uggagguggc caaaaagaug      360

uacgacgaag ggaucacacc cggagagcug ugccuccagc ugcuuaccgc uggcaaacgg      420

uauuucccug auuuuuaccg cagcgaccug caggccgaau uugauagaau uuggaauuuu      480

caaaagcagu ucuauccgga cucccucauc gauaagguga aagacgaggu ccggggcaag      540

aacaaaucuc agacgugggc gauucuggcc aaguacuuug uguggaaaga aguugagaau      600

agcuggaacg aggaagaggc ucagacccgc agaguggaaa aggaguaccg gcuugucggg      660

aucaaaagag gagugaagcg cgaggagcug aagcucgaga auuuccaaug gcggguuaaa      720

gcccuguccg aaagaaugaa ccccgaggaa cuggcuauug ugcuccagga gaucaaugaa      780

cagauuagca acucuuccgg cuaucugggg gccaucagcg aucgcucuaa ggagcuuuac      840

uuuaaccggc agacagucgg acaauaucag auggcggugc uggacaauaa cccuaauauc      900

ggccucagaa acaugguguu cuaccgccag ggguaucugg gagaguuuaa uaccauuugg      960

gaaaaacaag ccgaguucca caaggaacuu acagaggauc ugaaaaagga gauccgggac     1020

auugucaucu ucuaccagag acgccucaag ucccagaaag gccugauuaa caucugugaa     1080

uuugagcgga gacagaucga aguugagauu gacggcaaga aaaagaucaa aaccauuggg     1140

agccgcguga ucccccgguc uuccccucug uuccaagaau uuaagauuug gcagacgcuc     1200

aaugauaucg aggugagcgu ccugggagug aagaacaaaa gaaagaaaca ggacgauaau     1260

uccacaaccc uucuggacuc ugcugagaac aucgauagcc ucaagcugaa uguuucccgg     1320

ccccuugacg ccgaugaaaa gagccugcuc gcuaaggagc uguucauucg cgacaagcug     1380

acaaaaucug augugcucaa gcugcuuuuu aacaauccuc agaaccugaa ucucaacuuc     1440

aaaaacaucg acggcaauag aaccgggucc gcccuguauc aagccuuuag caagauucug     1500

gaaaucucug gacaugagag cauuaacuuc aaaaaguccg cugaugaaau cgucgaacag     1560

gugaaaacca uuuucucugc gcuuggcugg aauacagagg uucucugcuu cgacagcgaa     1620

aaggagcugg acaagcagcc guacuuuaaa cuguggcacc uccuguacuc cuucgaaggg     1680

gauagcacgc ccaccggaga cggcaaccuu auccagaaga ucgccgaucu guguggcuuu     1740

gagaaagacu augcuucuau ucucgccaau aucacauucc aagaagauua cgggucccug     1800

agcaccaagg ccauucggaa aauccugccu caucuuaagg acggaaacca guaugaugug     1860

gcuugcgagu acgccggcua uaagcacucu aaauccagcc ucaagaaaga ggaaauuaag     1920

aauaaagucc ugaaggacaa gcuggagcuc cugcccaaaa acucccuuag aaauccugug     1980

gucgaaaaga uccugaacca gauggugaau guuaucaacg cgauuaucac aaccuacggg     2040

aaacccgaug agauucgcau cgagcucgcu cgggaacuga agaaaaaggc caagaaaaga     2100

gaggaacuga cgaagacaau uguggacacc accaaaacac augagggaau ccgcgccauc     2160

cuucagaaug auuucggcau gucucacguc agcagaaacg cuauuauccg guauaagcuc     2220

uacgaggaac ugaaagacaa uggguacaag acccuguauu ccaacacaua cauuccgaaa     2280

gagaagcucu uuucuaagga uuucgacauc gaacacauua ucccucaagc ccgccuguuu     2340

gacgauagcu ucuccaacaa aacgcuugag guuaagagca ucaauauuga aaaaggcucu     2400

aagaccgcuu augacuuugu ggaggaaaaa uacggagagc agggccugca ggaguaucuc     2460

aacagaugug aagugcuguu ccgggauaag aagacaaaac ugcgcaaacu uaaaaugcaa     2520

gaguccgaaa uccccgaggg guuuauugac agagaucucc ggaauaccca guacaucgcc     2580

aagaaagcgc ugagcaugcu gaacgaaauu ugccgcagag ucguggccac aaccggagcu     2640

aucacggacg agcuccggga ggauuggcag cugguggacg uuaugaagga acuuaauugg     2700

gagaaguaua aaguccuggg ccucguggaa uacuucgagg auaacgacgg gcgccugauu     2760

ggaagaauca aggauuggac caaacggaau gaccauagac accaugccau ggaugcucug     2820

acaguggccu uuaccaagga cgucuucauc caguacuuua acaauaaaaa cgcgucucuu     2880

aagccugaca caaaugaggc cgcuauucgc aacaaguauu uccaaaaugg caaagccauc     2940

ccucccaugu cccuggauga auuucgggcu gaggccaaga aacaccucaa cgacacccug     3000

auuagcauca agacgaaaaa uaaggugguu acaauuaacc ucaacagaac caagaaaaag     3060

aaugggauca acaaaaagca gcagcagaca ccccgcggcc aacugcaccu ugaaaccgug     3120

uacggaucuc auaaacagua ugucgugaag gaggaaaaag uuaaugccag cuucgaugcu     3180

gcgaagaucg ccaccgugaa caagccggcu uaccgggacg cccugcucag acgccugcag     3240

gagaauggca acgauuccaa aaaggccuuc acagggaaaa auagccugga caagcagccc     3300

gucuggcuug augcuaaaca aaacgagugu gugccugaaa agguuaagac ggugaugcug     3360

gagaccgucu auacagugcg gaaacccauu gacgaaaagc ucaaugugga uaaagucguu     3420

gacgccagaa uccgcaagcu gcuccaggau cggauuaaag aguacggaaa cgcgaagucu     3480

gcuuuugcca aucuggacga aaacccuauc uggcuuaaua aggagaaagg cauuaccauc     3540

aagagagugu ccgugagcgg gaucuccaaa gcccagucuc ugcacgagaa gaaagacaag     3600

gauggaaacu ucauucucga caaggauagc cggcgcaucc ccgucgacuu ugugaauaca     3660

ggcaacaauc aucacguugc uauuuauaga aaaccggugc uggauaagaa agggcaagcc     3720

gucuucgacg aaaacggcga gcugaaguuu gaacuugagg aagugguugu guccuucuuu     3780

gaugcgguca cccgggugaa ccugucucag ccuaucauug acaaagauua caagcgcagc     3840

gagggaugga aauuccucuu uuccaugaag cagaaugagu acuucguuuu ucccaacgaa     3900

aagacgggcu ucgacccuaa agagaucgau cugcucaauc ccgaaaacua ugcucugauu     3960

ucuccuaauc uuuucagagu gcagaagauc gccagcaaag acuacgucuu ucggcaucac     4020

cuggagacag ugguggccga uagaaaggag cugcaagaaa ucaccuggaa acgcauuucc     4080

agccucucua uccugaaccg gauuaucaag gucagaguua aucacauugg gcagaucgug     4140

augaucggag aguauuga                                                   4158


<210>  4385
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4385
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

aucacggaug acuauaaagu uccuucuaag aaaaugaagg ugcucggcaa uaccagcaaa      120

aaguacauua agaaaaaccu gcuuggggcu cuccuguucg auuccggaau cacagccgag      180

ggcagacggc ugaagcgcac cgcgagacgg cgcuauacaa gacggcgcaa ucggauucuu      240

uaccugcagg aaaucuuuuc uaccgagaug gcuacacucg acgaugccuu cuuucaaaga      300

cuggacgaua gcuuccuggu gccggacgau aaacgcgacu ccaaguaucc caucuuuggg      360

aaccucgucg aagagaaagc cuaccacgau gaguucccua ccauuuauca ucugagaaag      420

uaccuugcug acucuacgaa gaaagccgau cugcggcucg uguaccuggc ucuugcccac      480

augaucaagu aucgcggaca uuuucugauu gaaggcgagu ucaauagcaa aaacaaugac      540

auccagaaga acuuucagga cuuccucgau accuacaaug cgauuuuuga auccgaccug      600

agccuggaga acucuaaaca gcucgaagag aucguuaagg auaaaauuuc caagagcgcc      660

aagaaagaca gaauccugaa acuuuucccc ggcgagaaaa auucugggau cuuuuccgag      720

uuccugaagc ucauuguggg aaaccaagcu gauuucaaaa aguauuuuaa ucuggacgaa      780

aaggccccuc uucacuucag caaagaaucu uacgaugagg accuggaaac acuccugggc      840

uauaucgggg augacuacuc cgaugucuuu cugaaggcua aaaagcucua ugacgccauu      900

cugcuuagcg gaauccugac cgugacagac aacggcaccg aggcgccccu cuccucugcc      960

augauuaugc gguacaaaga gcaugaagag gaucuggccc uucugaaggc uuacauccgc     1020

aauaucagcc ucgaaacgua uaacgagguu uucaaggacg auacaaagaa cggguacgcu     1080

ggauauauug acggcaagac cucccaggaa gauuuuuacg ugcaccugaa aaagcugcuc     1140

gucggccugg agggggccga cuauuuccuu gagaaaaucg auagagaaga cuuucugcgg     1200

aagcagagaa cauucgauaa uggaagcauu ccuuaccaga uccaccucca agagaugcgc     1260

gccauucugg acaagcaggc uaaauuuuau ccguuccugg cgaagaacaa agaacggauc     1320

gagaagaucc uuaccuuucg cauucccuac uacgugggcc cucucgccag agggaauagc     1380

gauuucgcuu gguccauccg gaaaagaaac gaaaagauua cccccuggaa uuuugaggac     1440

gugaucgaua aagagagcuc ugccgaagcc uucauuaacc gcaugacauc uuucgaccug     1500

uaucugccug aggaaaaggu ccuccccaag cauucccugc uuuacgagac guuuaauguu     1560

uauaacgagc ugaccaaagu gcgguucauc gcugaaucua ugagagacua ccaguuucuc     1620

gauagcaagc agaaaaagga cauugugcgc cuguauuuca aaaauaagcg gagagucaca     1680

gauaaggaca ucaucgagua ccugcacgcc auugauggau augacggcau cgaacuuaaa     1740

gggauugaga agcaauuuaa cuccagccuc ucuaccuacc augaucugcu gaauaucauu     1800

aacgacaaag aguuccucga ugacuccagc aaugaagcga ucaucgagga aauuauccac     1860

acacugacca uuuuugagga ucgcgaaaug aucaagcagc ggcuuucuaa auucgagaac     1920

auuuuugaca aguccgugcu gaagaaacuc agcagacggc acuacacggg auggggcaag     1980

cuguccgcua aacugaucaa uggcauccgc gaugaaaagu cugggaacac aauucuugac     2040

uaucucaucg acgauggagu uagcaacaga aauuucaugc agcugauuca ugacgaugcc     2100

cuguccuuua aaaagaagau caaaaaggcc caaauuaucg gcgacaaaga uaacaucaaa     2160

cagguggucc aggcucuccc ggggcggccu gccauuaaga agggaauccu gcagucuauu     2220

aaaaucgugg acgagcuugu uaaggugaug ggcagacgcc ccgagagcau ugucguggaa     2280

auggcucggg agaaucaaua caccaaccag gggaagucca auagccagca gagacugaaa     2340

cgccucgaag agucucugaa ggaacugggc uccaaaaucc uuaaggagaa cauuccugcc     2400

aaacucagca agaucgauaa uaacgcgcug caaaaugaca gacuguaucu cuacuaucug     2460

cagaacggaa aggauaugua caccggcgag gaacuugaca ucgaucggcu gucuaauuau     2520

gacauugacc acaucauucc ccaggccuuc cucaaagaua acuccaucga caauaagguc     2580

cuggugagcu cugcuagcaa ccgcgggaaa uccgaugacg uucccagccu ggaggugguc     2640

aagaaaagaa agacacuuug guaccagcug cucaagucua aacugauuuc ccaacggaag     2700

uuugauaauc ucaccaaagc cgaacgcgga ggccugagcc cugaggcuaa ggccggguuc     2760

auccagagac agcuugugga gacacggcag aucacgaaac auguugcgcg ccugcucgac     2820

gaaagauuca acaacaagaa ggaugaggac aauaaaaccc ugcggacagu gaagauuauc     2880

acccugaaau ccucucuugu cagccaauuu cgcaaggauu ucgaacugua caaagugaga     2940

gagauuaacg acuuucacca ugcccacgau gcuuaucuca augccguggu ugcuuccgcc     3000

cugcucaaga aguacccgaa acuggaaccc gaguucgucu auggagacua cccuaaguau     3060

aacucuuuuc gggagagaaa aagcgccaca gaaaaggugu acuucuauuc caauaucaug     3120

aacauuuuua aaaagucuau cagccuugcu gauggcaccg ugaucgagcg cccccugauu     3180

gaagucaaug aggaaacggg ggaguccgug uggaacaaag uugcggaccu caauaccgug     3240

cggaaggucc ugagcuacuc ucaggugaac aucguuaaga aaguggagga acagaaucac     3300

ggccuggaca gaggaaagcc uaaaggccuu uucaacgcca aucuguccag caagcccaaa     3360

ccgaacucua aggagaaucu cgucggggcu aaggaauacc uggauccuaa aaaguaugga     3420

ggcuacgccg ggauuuccaa cagcuuugcc gugcucguua aaggaacaau cgagaagggc     3480

gcuaaaaaga agauuaccaa cauccuggag uuccaaggga uuucuauccu ugaccgcauc     3540

acauaucgga aagauaaguu uaauuuccug cuggaaaaag gcuacaagga cauugaucuc     3600

aucauugagc ugcccaaaua uucccuuuuc gaacugagcg acggauccag acgcaugcuc     3660

gccagcaucc ugucuaccaa caauaagcgg ggcgagauuc auaaggggaa ccagaucuuu     3720

aucagccaga aauacgugaa gcuccuguau cacgcgaaaa gaauuuccaa uacguucaac     3780

gauaaucauc gcaaguacgu cgaaaaccac aaaaaggagu uugaggaacu uuucuacuau     3840

auccuggagu uuaaugaaaa cuacguggga gcuaaaaaga auggcgagcu gcucaagucu     3900

gccuuccaga gcuggcaaaa ccacuccauu gacgaacugu gcucuagcuu uaucgggccu     3960

acaggauccg agcggaaagg ccuuuucgag cugaccucua gagggggcgc cgcugauuuu     4020

gacuuccucg gagugaagau uccccgguau cgcgauuaca caccuagcuc ccugcucaaa     4080

gacgccaccc ugauccauca gagcgucacc ggccuuuaug aaacaagaau cgaucugggg     4140

aagcugggag aggacuga                                                   4158


<210>  4386
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4386
augaagaaca aaaccuggua ccugggccuc ggggugggac ccacaagcau cggcugggcc       60

gcuacggacu ccaauuauaa gcugcuucgg aaaaacaaga aacggcugug gggaguccgc      120

cucuucgagg aagccaagac cgcgguugau agacggacau uucgcgcuga gagacggcgc      180

cuggccagac ggaaguggcg cauuucucuu cuggaagaac ucuucgccaa agaagugagc      240

aagaaugacc cuaacuuuuu ccugagacug aaagagucca aguaccacuu ugaggauaaa      300

ucucucauga augaauauau ccuguucaac gacaaggauu acaccgacaa ggauuauuac      360

aaagaguauc cgacaauuua ccaucuucgg cugagccuca ccacagaaga gaaucccgac      420

auccgcaagg uguaccuggc uguccacucc augcuuaaaa acagaggaca uuuucugcuc      480

cagggccaau cuuucaauga uagcaacauu gacuccguga ucaaggagcu gcuggaacuc      540

gagaccguua aaaucaauuu ugaagugacg gaugagguca uucacagccu gaagaaaauc      600

gcccuugaaa agaagaaccu gucugacaaa aauaacgaug ugaagaaacu cuauccuaag      660

gagaaacagc ugcaggagau uuucaaacuu guuuuugggg gaaagaccuc ccuggacaaa      720

cucuacgaca ucgaagagua uaaggaacug gauaauagcg ugaaaucuau uuccuucaag      780

gagaaagucu acgaagaggu gcggcccgac uaugagcaga uccugagcaa cuacauugaa      840

cuccuggauc uugcuaagcu gguguaugac ucuaucgucc ucgccgauau caagaaagag      900

ggcaagacaa uuuccgaaag caaaguugag cuguacaaca agcauaaaga cgaucuugcg      960

aaucugaaga agcucgugaa aaucgacucc aagcugucug aagauaaaaa gaaagaggug     1020

uacuuuacca uuuucaagga ggacaaagaa aagggcacaa acuaugucaa uuacacccac     1080

cgcagccaag aggggaaguc cugcagcuau gaagauuuca aaaaguuucu gaaaaaggag     1140

cucgagaaac uggaagacuc uccuacgaag acaaccaucc uugaggaacu ggaccucgag     1200

acauuccugc cccugcagag aaccaaggau aacuccgugg uuccuuacca gauucacaaa     1260

gaggagcuug ugaagauccu cgacaaugcc gcuaaauauc auagcuuucu ggccgagaag     1320

gaugacucug gauacagcac ccgggaaaaa gucgugcagc uguucgaguu uagaaucccg     1380

uauuacauug gcccccucaa cgcucaccau aauacagaaa acgggggaua cuccugggcc     1440

auccgcaaga agggcaugga gucuguuccu gugacgcccu ggaauuucaa agaugucauu     1500

gacgaaagcg cguccgccga ggcuuuugug aacaaucuga agaacaaaug ugccuaucuu     1560

agcggggagg auguccugcc uaaaaauucu cuccuguacu ccgaauaugc ucugcuuaac     1620

gagcucaaug cccugcggua cgaaggaaac agaaucagcg uggaggcccg cgacguuauu     1680

gugaccaagc uguucaagga acaaggcaag aaagucacaa agaaaaccau caaggagcuc     1740

cugaaaacag agggcuuuau cgaugggaag ggagaaauug ugggcaucga caacauuguu     1800

aaaaaugauc uuaagucuua ucgggacuuc aagaaaaucc uggcugauaa guuugacaaa     1860

gagagagugg aaaacauuau ccucuggauu acccuguacg gggagucccg caagcugauc     1920

aaagagaaga ucacgaaggu cuauggagac uucuacacag uggaugaaau ugagaaaaug     1980

agccggcuua ccuacaagga cuggggcauc cucuccagaa aacugcugac cgaacucgug     2040

ucugagaagc uguauaauga agagacaggg gagugccuua acauuaucgg agcgaugcgc     2100

cagggcacca uucuguuuau ggaacuccug gccgauaaau ucgacuacau gagccagauc     2160

aagaaucaca acaaggagaa uaaagaugac aucacagaaa uuacgcccga uauccuggag     2220

aaccuucacg cuuccccggc cguuaagcgg ucuauuuggc agaccgucag aaucguggag     2280

gaacucaaaa caauuaucgg cugugugccu accaagaucu ucauugagac aaccaaacgc     2340

aauaacgccc ccuauaaggu cccuacgcug aaucggaacg accuggugac caaguacaaa     2400

acaaucagag aacaugagau uuuugaacuc gagaaggaac ugaaaaauac ccuugauuuc     2460

acacugaaca agaaucggcu caucaacgag gagcccagca aacugaaggc uaccaagcug     2520

uaucuuuacu auacgcaacu ggggcgcugc auguacacag gaaagaagau ugaccucggc     2580

gaacuguuug auaacaauaa caaauacgac aucgaucaca ucuaucccca guccaagguu     2640

aaagacggga gcuucaauaa cauugugcuc gucaccaagg ccucuaauga caugaaagcg     2700

gauguguuuc cucugaaguc caaguuccag acaaaagaga acaagaucuu uuggagauuc     2760

cuuaaagaaa agaaacugau uaccgaggag aaguacaauc ggcucguuag aaccgaagag     2820

uuuagcaacg gagaacugac aggcuucauc acgcgccaac ugguggagac cucucaggcu     2880

auuaagguca ucuccaaugu gcuucgggaa cugaacccgg agacaaccau uuguuauagc     2940

aaagccgaga auguuucuag cuuuagacag uccuucggga agaucaaaga agccaaccgc     3000

aaggcugaga auaacgaaaa acucaucaga gugcaggaga uuaaugacua cuauaacgcc     3060

aaggaugcuu accugagcau cgucgugggc aacguguaug acgucaaguu uacacggaau     3120

guucauaacu ucauuaaaaa uaacaaggau gcccgcagau acucucucaa uggacuguuc     3180

uacaccgacg ugaaaaacgc gaauacgauc gccugggaaa uggauaagac agugcacguc     3240

guggagaaaa ccaugaacaa uaacaauauu cuuaucaccc ggcgcacauc cgagcaaaag     3300

ggcgcucugu augacgccac ccucuacaag gcuaaaguug ccgaaaaggc gaaagagggg     3360

acauauuacc cccugaagac gaccgauagc aaaaucaagg acgugggaaa auauggcggg     3420

uuuacaaaga uuaagaucgc cuacuauucc auuuucgaau acacccugga ugggaaaaag     3480

ggcaaacaga agaucacaag aauugucccu auccccaucu acauuucuca gaacaucaaa     3540

gacgacaagg cccuucugga guaugcucag acccggcucc cugggaagga ucugaaaauu     3600

aaguaccgca aacucugcau cggcagccug gugaagauua auaacuuuaa uuauuacauc     3660

ggaggcaaaa cgaacaauaa cuucaccuuu gacuccgcug uucaagugau ucuugauaag     3720

gacucugaaa gcuauaucaa ggagcuggcc aaauaccuga acuggaagaa agagaauaag     3780

gaugggaaac ucugggaauc caucacagag gacaagaaca uucagcugua ugauucucuu     3840

gucaagaaaa ugaauaccgg aaucuucauu agaaagacac ccaacaaaua caccgaacug     3900

cucacacugg aggugaagaa uaaauuuauc aacauugaca ucgaugagca gguuaagcuc     3960

cugcuugaaa uccugaaucu gcucaccaac aaaaaguuca cgagcaagcu gaaugagauu     4020

ggcaucacag ccuccagagg gguguguagc uuuaaccuuu cuaaucaguc ccaauucagc     4080

auuaucgaac agucugucac cggaguguac gagaaagaaa agcugauuau cgagaaaaag     4140

aaagagggca uccucuga                                                   4158


<210>  4387
<211>  4161
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4387
augaaccaga aguacagcau cggccuggac auugggacca auuccguggg augggccguc       60

aucacagaug acuauaaagu ucccgcuaag aaaaugaagg ugcucggcaa cacggauaaa      120

caaucuauua agaagaaucu gcuuggggcc cuccucuucg acagcggaga gaccgcggaa      180

gcuacaagac ugaaacggac cgcccgcaga cgguacacac gcagacggaa ccgccuuaga      240

uaucugcagg agaucuuugc cgaagagaug augcaagugg augaauccuu cuuucagcgg      300

cucgacgauu cuuuccuggu cgacgaggau aagcgcggcg agagacaccc uauuuuuggg      360

aauaucgcug ccgaagugaa auaccaugac gaguucccga ccaucuauca ucugcggaag      420

caccucgcug auauuagcca gaaagccgac cugcgccuug uuuaccuggc gcucgcccac      480

augaucaagu uuagaggaca uuuccugauu gaaggccagc uuaaggcuga gaacacaaau      540

gugcaggccc uguuuaaaga uuucgucgag guguaugaca agaccguuga agagucccac      600

cucucugaaa ucacggugga cgcucugagc auucugaccg agaaagucuc caagagccgg      660

cgccucgaaa accugaucgc ccacuacccc gcggagaaaa agaauacacu uuuuggcaac      720

cugauugccc ucuuccuggg gcuucagccu aauuuuaaaa ccaacuucca acugucugag      780

gaugcuaagc uccaguucuc caaggacaca uacgaagagg aucuggaagg acugcucggc      840

gagguggggg acgaauaugc cgaucuguuu gcuagcgcca aaaaucuuua cgacgccauc      900

cugcucucug gaauccugac cguggaugac aacuccacga aggcuccccu uagcgcgucu      960

auggucaaaa gauaugagga gcaucagaag gaucugaaaa aguucgaaga cuuuauuaag     1020

guuaaugccc ucgaucagua caacgcuauc uucaaagaca agaacaaaaa gggcuaugcc     1080

ggguacauug aguccggagu gaaacaagac gaauuuuaua aguaccugaa gggcauccug     1140

cuccagauua auggcagcgg ggauuuccug gacaaaaucg aucgggagga cuuucuuaga     1200

aagcagcgca cauucgauaa cggauccauc ccucaccaaa uucaucugca ggaaaugcac     1260

gccauccucc ggagacaggg cgagcacuac cccuuucuga aagagaauca ggacaagauu     1320

gagaaaaucc ugaccuuccg cauuccuuau uacguggggc cgcuugcucg gaagggaucu     1380

agauuugccu gggcggaaua uaaggcugau gaaaagauca cacccuggaa cuucgacgau     1440

auccucgaca aggagaaaag cgccgagaag uuuauuaccc gcaugacccu gaaugaucug     1500

uaccucccug aagagaaagu ccugcccaag cauucccuuc uguaugaaac auucacggug     1560

uacaacgagc ucaccaaagu uaaguaugug aaugaacaag gcgaggccaa guucuuugac     1620

gcuaacauga aacaggagau cuucgaucac gucuuuaagg aaaaucggaa agugacaaag     1680

gacaaacugc ugaacuaccu uaauaaggag uucgaagagu uuagaauugu uaaccucacc     1740

gggcuggaca aggaaaauaa aguguucaac agcucucugg gaacauacca ugaucuccgc     1800

aagauccuga auaaauccuu ucuugacaac aaggagaacg cccagauuau cgaggauauu     1860

auccagaccc ugacgcuguu cgaagaccgg gagaugauca gacaacgccu gcagaaauau     1920

agcgauauuu uuacaaaggc ucagcugaag aaacuugaac ggagacacua caccggcugg     1980

ggccggcucu cuuauaagcu gaucaauggg auucgcaaca aagagaauaa gaaaaccauc     2040

cuggacuacc ucauugauga cggauaugcc aacagaaauu ucaugcagcu gaucaacgau     2100

gacgcgcuua gcuuuaagga agagaucgcc aaggcucaaa uuaucggcga ugucgacgau     2160

auugccaaug ugguccauga ccugccuggg ucccccgcua ucaaaaaggg gauucuccag     2220

ucugugaaaa ucguugacga gcuggugaag gucaugggcc acaacccggc caauaucauu     2280

aucgaaaugg cgcgggagaa ccagacaacc gauagagggc gccggaauag ccagcaaaga     2340

cugaaacuuc uccaggacuc ccugaagaac cuggauaauc cugugaacau uaaaaauguu     2400

gaaaaccagc aacuccagaa cgaccgccug uuccuuuacu auauccagaa uggcaaggau     2460

auguacacag gagagacgcu ggacauuaac aaucucagcc aguacgauau cgaccacauc     2520

auuccccaag ccuauaucaa ggaugacucu uuugauaaca gagugcugac cuccagcucu     2580

gagaaucggg gcaaauccga caacgucccu agcauugaag uggugugcgc ucgcaaggcc     2640

gacuggauga gacugcggaa agcugggcuu aucucccagc gcaaguucga uaaucugaca     2700

aaagccgaga gaggaggccu caccgaaaac gacaaggccg gguucauuaa gcggcagcug     2760

guugagacac gccagaucac caaacauguc gcucaagugc ucgaugcgag auuuaaugcc     2820

aagcacgacg aaaacaaaaa ggugauucgg gaugucaaaa ucaucacgcu gaagucuaau     2880

cuugugagcc aguuccgcaa ggacuuuaaa uucuacaagg uuagagagau uaacgauuau     2940

caucacgcuc acgacgccua ccugaaugcc gugaucggaa ccgcucuccu gaaaaaguau     3000

cccaaacugg ccuccgaguu ugucuacggc gaguucaaga aguaugaugu gcggaaauuu     3060

auugcgaagu cugacaaaga aaucgggaag gcuacagcca aauacuucuu uuacagcaac     3120

cuuaugaauu ucuuuaagaa ggaaguuaaa uucgccgaug gcaccguggu cgagagaccc     3180

gacauugaaa cauccgagga uggagagauc gcuuggaaca agcagaccga cuucaaaauc     3240

gugcgcaagg uucugagcua uccucaggug aacauuguca aaaagacgga agugcaaaca     3300

cauggccucg accgggggaa accgagagga cuguuuaaug ccaaccccuc uccuaagccc     3360

aagccugauu ccagcgagaa ucucgugggc gucaaacgca accuggaccc caagaaauac     3420

gggggauaug cgggcaucuc uaauuccuac gcuguucuug ugaaggccau uaucgaaaaa     3480

ggggugaaga agaaagagac caugguccug gaguuccagg gcauuagcau ccucgaucgg     3540

aucacauuug aaaaggacaa aagagccuuc cugcugggaa agggcuauaa agauauuaag     3600

aagaucauug agcuuccgaa auacucucug uuugaacuca aggacgggag ccgccggaug     3660

cuggcuucca uccucagcac caacaauaaa agaggagaga uucacaaggg caacgaacug     3720

uucgugccuc agaaauuuac cacacuucug uaucaugcca agcgcaucaa uaaccccauu     3780

aauaaggauc acaucgagua cguuaaaaag caucgggacg auuucaaaga gcugcucaac     3840

uacgugcugg aauuuaauga gaaguauguc ggggcuacga aaaacggaga aagacuuaag     3900

gaggccgugg cggacuucga uucuaaaucc aaugaagaga ucuguaccag cuuucugggc     3960

gccguuaacu ccaagaaugc uggacucuuc gagcugacau cucucggcag cgccuccgac     4020

uuugaguucc ugggagugaa gauuccucgg uaccgcgauu auacccccuc uagccuucug     4080

aaagacucca cacugaucca ccaaucuauu accggccucu acgaaacgag aaucgaccug     4140

agcaaacuug gggaagauug a                                               4161


<210>  4388
<211>  4161
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4388
augaccaaga ucacagagga cuacauugug ggccuggaua ucgggacgaa cagcugcgga       60

ugggucgcca uggacuccaa aaauaccauu cucagaaugc acggcaagac agcuaucggg      120

ucucaucugu ucgaugccgg aaacagcgcg gcugaccggc gcgccuuuag aaccacacgg      180

cgcagaauua aacggcgcaa guggagacuu aaacugcucg aagagaucuu cgauucccac      240

cuggccaagg uugaccccua cuuuuucgcu cggcuuaagg aaucuggccu gagcccucuc      300

gauacccgca aaaauguguc cucuaucauu uauccgacaa gccuggagga caagaaauuu      360

uauguggaua accccaccau cuaccaucug agauauaagc ucaugacgga agaccggaaa      420

uucgaucugc gcgaggucua ccuugccauu caccauaucg ugaaguauag agggaauuuu      480

cuguacaaca ccccuguuaa ggcuuucgag gccuccaaaa uugacgugaa gggaagccuc      540

aaaaagcuga augaacuuua cgagggccug gauucugaau uuaacgucga gcucgacucc      600

aauaacgcgc uggagaucga aaauauuauc cgggacaaaa agaucuucaa aauuaacaag      660

gugaaggaga ucaaucagcu gcucagccug aaaacagaag auaaggagcg caccaaacuu      720

augaaggacg uuucuaaaca aauugugaac gccauccugg gcuauaagac aaaguuugaa      780

accauucucc ugaaagaugu cuccaaggac gaggcugaug acugggaguu caaacuuacg      840

gauguggacg ccgauaauaa guuugaaaac cugaucgggg accucaacga gaaugaacag      900

gagauccugc uggugauuag aaaacucgcu aacgccauca cacugagcaa uauugucgaa      960

gagggaaaga cccuuucuga guccaugaag gauaaauaca agaaacacag cgacgaccug     1020

aagcuccuga aacagguuau cuccgaucac cccgaccggg auaaggcgaa gaaacuugcc     1080

cuggcuuaug accucuacgu gaacaauaga cauggcaaga uucugcaggc caaagauauc     1140

cuggaaaaga aaaagacacu cucuaaagag gacuucuaua aggaaaucaa gaaaaaccug     1200

gaugacagca aggaguccca agaaauucuu gaugcuauca gccuggacuc uuucaugccu     1260

aaacagcgca ccaaugagaa cggggugauu cccuaccagc uccaccagcu ggagcuggau     1320

aagaucauug aaaaucaagg aaaauauuac ccuuuucuua aggaggucaa cccggugucc     1380

agccaucuca agcaggcccc cuacaaacug gacgaacuga uccgguucag aguuccuuau     1440

uacgugggcc cccucauuuc uccuaaugag agcaccaaga acaaacagac aaagaaaaau     1500

cagaacuuug ccuggaugau ccgcaaggag caagggcaga ucacgcccug gaauuucgau     1560

cagaaggucg accggauggc uuccgcgaac aaauuuauua agagaaugac cacaaucgac     1620

accuaucugc uuggagaaga ugugcugccg gccaacucuc uccuguacca aaaauucaca     1680

guucugaaug agcuuaacaa ugugaagauu aacggcaaac gcaucagcgu cccucucaag     1740

caggaacugu auaauaaccu guuuaagaaa aauuccaccg ugacgacaaa caagcucaaa     1800

agcuaccuga aggagaauua uaaacuuauu aaggucgaaa ucaaaggccu gucugacgag     1860

aagaaguuca acuccgggcu caccaccuac aauaaacuga agaaccugaa aaucuuugau     1920

cagcagauug acgaucccaa uuacaacaag gacuucgagc ggaucauuga auggagcaca     1980

aucuuugagg auaaagcuau uuauaaggaa aaacuuaaaa ccaucgacug gcucucugau     2040

agacaaaucg acaagcuguc caaaauucgc augcagggau ggggccagcu gagcaagaaa     2100

cuccuguccc agcuuacaga uaauaacggg caaacgauca uugagcagcu gugggacucu     2160

cagaacaauu ucaugcagau cgugaaccaa gccgauuuua aggacgccau ugcuguugcc     2220

aaucagaacc uccuggugga caccagcguc gaagauaucc ugaaugaggc guacacaucc     2280

ccugcuaaca agaaagccau cagacaggug guuaaggugg ucgacgauau ugugaaagcc     2340

accucuggaa aggugcccaa acagaucgcu auugaguuca cacgggacgc cgaugacaau     2400

gcuaagauca gccaaucccg cgccaacaaa cuucggaaag uuuauaagga aaugagcaau     2460

gagcucgcgu cugaaaccau uagaaacgag cuggaacggg ucgccaaaga ucagaagcug     2520

cucaaagaca agcacuaccu guauuucaug cagcuuggcc gcgaugcuua cacgggggag     2580

cugaucgaca uugauaaucu cgagcaauau gacaucgacc acgugcugcc ccaguccuuu     2640

aucaaggaug acagccugga aaacagagug cuugucaaaa aggccgugaa uaacggaaaa     2700

ucugauaaug uuccugugaa gcuguacggc aaccggaugg cuaccgaccu cggcaugaca     2760

auuagacaua ugugggagaa auggaaggaa caggggcuga ucaccaaaac aaaguacaac     2820

aaucucauua ucgauccgga caagauuaac aaauaugagu ccagcggauu cauccaccgc     2880

cagcuggucg agaccucuca aaucauuaaa cuugugagca cgauccugca guccaaauac     2940

cccgauacag aaauuaucgu ugugaaggcc cgguauaauc auuaccucag aaaacgccug     3000

aaccuguaua agagcagaga ggucaaugac uaccaccaug cgauugaugc cuaucuuucu     3060

gcuaucugug gcaaccugcu cuaccaggug uacccuuauc ugcggcccuu uuucguuuac     3120

gggcaguaua agaaauuuuc cagcgaaccu gagaaggaaa agaucauuua cgacaagacc     3180

cgcagauaua auuucaucuc ccaaauuuuu gagaacaaag auaaugacau cauuucucgg     3240

gagacaaaga agaaaguguu cgauaagaaa gacaucauug aaaagcucaa acgcgccuac     3300

aacuauaagu acaugcuggu cagcagagag accgaaaccc acgaccagga gauguuuaag     3360

augacagugu acccccggcu uucccgcgau acgaaaaagu cuagaaaucu gaucccgaaa     3420

aagaaagaca ugccuaccga aaucuaugga ggcuacacaa acaauagcga ugcuuauaug     3480

auuaucgccc ggauuaacaa gaaaaagggg cccgaguacc gcguguucgg cgucccuaug     3540

agagagcucg uuaaucugcg gaaggccgaa aagaacggac acuauaaggc uuaccugaaa     3600

caggugcuug agcccgaaau cauguauucc aagaauggca aaaagaacaa gaccauuucu     3660

agcuuugaga ucgugaaagc gaaggucccu uacaaacagg ugauccugga cggggauaag     3720

aaauucaugc ucgccuccag cacauacguu caaaacgcua agcagcugac ccucucucag     3780

uauuccaugc aagccauuac ggacaauugc gaaaacgaua cagaggagga aaaggcccug     3840

guggaggcuu acgacgaaau ccuuaccaau auugauaaau aucugccccu guuugacauc     3900

aacaaguuca gagagaaacu ccaugccgga cgcgagaagu ucgucgaucu gccgcuugcg     3960

gaaaagaagg agaccauucu ccaggugcug aauggccugc acgacaacgc uguuaugccu     4020

aagaucaaaa gccucgggcu gucuacaccc cuuggauuua ugcaguucca uaccggcgug     4080

ucccugagcg aaaaugccaa gcugaucuac cagucuccca cagggcucuu ugagaaacgg     4140

cugaagauuu ccgaucuuug a                                               4161


<210>  4389
<211>  4164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4389
augagcgacu ccaagaaauu cgagggcgaa aacgugaagu cuggggauaa aaagaccaau       60

aaaaaccacg gaaagguccu uaagagcucc cccaaagaga ucagaaagaa uacagaauac      120

uuuauuggcc ucgacaucgg gacgucuagc guuggauggg ccgugaccaa cacagauuau      180

gagauucuga aauucaaugg caagcggaug uggggguccc gccuuuuuaa cgaggcuucu      240

accgccgaag agagacgggg acaucgcgug aauagacggc gcauccagag acggcgcgac      300

agacugaacc uccuggaaga gauuuucagc gaagagaugg cgaaagucga uccugaguuu      360

uucaugcggc uuaaggaauc caaguuucac cuggaggaca aagaugaacg cauccucggc      420

gagagcaaua ucauuuucaa gggcucugaa gacggggaua aaaaguacua ugagaaauac      480

ccgacaaucu aucaucugag acacaagcug augcaauccu gcgaggacgu ggauauucgg      540

gaacucuaca uggcucugca ucacaucauu aaauaucgcg gacacuuucu uuucaagggc      600

gacuuugagg aaaccgggac aagcaucgag aacauuauca ucgaacugcu cgagcugauu      660

aauaacaauc uuggaguuaa gaaauucgag uacaacgaau cucugaucaa ugagcucaag      720

aacauugugu ccaacaccag caauacgauc accaacaaaa agaaacugcu gggcgaauuu      780

uucaauaaga aggaguacaa agcccauaag ucucucuuca uucuguccau cggggccagc      840

gucaaacccg uggaucuuuu ucuggaagag gaguauaagg acaucgacga uauuaaaaac      900

aucuccuuca agaaucucga auacgaccag guuagagaua cauaugaguc uauuuuucug      960

gacaagaucg aguucauuga uguggcuaaa cggcuuuacg acgccaccau ccugcucagc     1020

aucaagccug aaggaaaaac acuguccgag gcuaaggucg auauuuauaa caaacaccau     1080

aaugaccugg aacuccugaa gaaggugcuu aaaaccgaua aggagaaaua cgacagcauc     1140

cugaagcucg auauuaaaaa gggcaacaau uaugugaacu acgucaaaaa gucuaagaau     1200

ggcaucuccu guagcaaaga agaguuucac aacgcccuga agaaaguucu ugugaagauu     1260

gaaaagacgg acgacaucaa guacauucug aaugagaucg agcucgaaag auuccugccc     1320

cugcagcgcg ugaaggauaa cucugucauc ccuuaucaga uucaccggua cgagcucaaa     1380

aagauccugg aaaaugcgag caaaaaguuu acauuccuua acgaggaauc cgacgggcau     1440

accaacauug agaaaaucau uaagaucaug gaguauagaa uccccuacua ugugggaccu     1500

cugaaugauc accauucugu uuacaacaau ggcuuugccu gggugagccg caagcggucc     1560

gggagaauua caccguggaa cuucgacgaa auggucgaua aagaggaaag cgcugagcgc     1620

uuuaucgaaa aucucaccaa caagugcucu uaucugauug gagaggacgu gcugcccaaa     1680

gagucccuuc ucuacagcga guucucucug cugaaugaac ucaacaauau caaguacgau     1740

ggcauuuccc ugccuaucga caucaaaaag agcauuguug aaaagaucuu ucggguggag     1800

aaaaagagag ucaccaaaaa guauauuaaa gaguuccuua agcaagaagg guacuccgau     1860

ggaaaaggcg aggugucugg caucgacauu gauaucaaga gcgaccugaa guccuaucgc     1920

gauaugguca aaaucuucgg gacagaaauu gacuaccgga uggccgagaa caucauuaag     1980

ugggugacgc ucuuuggaga cucuaaagau cugcugaaga ccaaaaucaa uaaggaauau     2040

ggcgagaagg uuagcaaaga ccagcuuaag aaagugugua agcucaaaua cagagauugg     2100

gggcgccugu ccaagaaguu ccugacaggc auucggagcu cuguccucgu gaacgacgcu     2160

accggagagg gguauacaau cauugaagcc augagaaaua ccgauaacaa ucugaugcag     2220

cuucugcgga acgaguacga cuaccucaaa aagaucgauc agaucaacau gcugucccgc     2280

aaagacauug augaaaucag ccacgacauu cuggaugacc uuuauugcuc ucccgcgguu     2340

aagagauccc ucuggcaaac gaucaaaauu guggaggaaa ucaagaagau caugggccau     2400

gagccuaaaa aggucuuugu ggaggugaca cggaccaaua aagccgauaa gaaaaccaca     2460

auuaccagaa agaaccgccu gcuggaacuc uacagcgcua ucaaugccga gucucgggac     2520

uggguuaacg aacugaauag cacagagguc uccaaguuca acagcaaaaa gcuguaucug     2580

uacuauacgc agaugggaaa auguauguac accggcaaga auauugacau cgaaucucuc     2640

uuuacagaua uguaugacau ugaucacauc uuccccagau ccaaaaccaa ggacaacagc     2700

uuugagaauc uggugcuggu gacacgcaaa gcuaacgccg auaagagaga cgucuacccg     2760

aucgauccua agauucagac cgagcggaaa ucccuuuggg acaugcucca cgauaagaaa     2820

cugaucgggg acaagaaaua cgaucgccug acgagaacca caggauucac cgacgacgaa     2880

augucuggcu uuauugcccg gcagcucgug gagacaagcc aggcuuccaa ggcggccgcu     2940

gaaaugcuga aacuuaucaa uaacgauucu gagauuguuu augugaaagc cgagaauguc     3000

agcgacuucc gccaugaaaa cgaguuuauc aaggugagag aaauuaauga ucaccaucac     3060

gccaaagacg cuuaccugaa caucguugug gggaacgucu auaaugagaa guucaccaaa     3120

aaccccgcca auuacaucaa gaagggaggc gggaacaauu auucccucaa cugcauuuuu     3180

aaucggaacc uggaucgcaa uggcaaaaga aucugggacg aaaagaaauc uauugauacg     3240

gugaaccgga ugaugaaaag caaugacguu cgcgugacau ucaaaaacag agucgauaag     3300

ggaccuaucc ugaauaaggu gaccauucac aaagcguccg aguucaugga cgauggcaag     3360

aaaaacaaga aaggguuucc caucaagagc ucugagaaaa auggaaacaa ucggcuuugu     3420

aaccuguaca aguauggcgg guacaaggac cucaacuacu cccauuauac aaucgugaga     3480

uacaaugucu augaaaagaa gggacuggau auuaaauacu auaugauccc cauuccuuac     3540

uaucucugcc uguguaacau caaucaacac gacuacguuu acgacuauau uagcaaccgc     3600

ucuaagaaag augugaauaa caucgagauc auugugaaug aacuuaagau gggcucccug     3660

aucaacauug acggguacaa uuauuaccuc ggcggaagca agccgaaaac cuucuauguc     3720

gaucccgcuu accagaucau ucuggacaag gagaucgaac ggugcauuaa agagaucgug     3780

aaggccaaaa acaaugagua uagauuuauc aacaccgaua agauuaauaa gaaccugaau     3840

augaaaaucu acaaguacau uguuaacaaa aagaucauug gcguguuccg caaccggaaa     3900

ugggacaagg gggaauaucu uaauucuucc gagacaaucg aaaaguuuag cggacugucc     3960

gucgagagcc aauguaacgu gauccucaga cugauuaaug auaugcgcgg cucuaacaaa     4020

gggggagcca auagcaaguu cgacgaacuc gagaugacga ucucccgggc ugagauuccu     4080

cagacccugu cuaacguuag cuccuuuaaa aucauuaauc agucuaucac aggccuuuuc     4140

gaaaacgagg ugaccaucgu cuga                                            4164


<210>  4390
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4390
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

aucacggaug acuauaaagu uccuucuaag aaaaugaagg ugcucggcaa uaccagcaaa      120

aaguacauua agaaaaaccu gcuuggggcu cugcuuuucg auuccggaau cacagccgag      180

ggcagacggc ugaagcgcac cgcgagacgg cgcuauacaa gacggcgcaa ucggauucuu      240

uaccugcagg aaaucuuuuc uaccgagaug gcuacacucg acgaugccuu cuuucaacgg      300

cuggacgaua gcuuccuggu gccggacgau aaacgcgacu ccaaguaucc caucuuuggg      360

aaccucgucg aagagaaagc cuaccacgau gaguucccua ccauuuauca ucugagaaag      420

uaccuugcug acucuacgaa gaaagccgau cugcggcucg uguaccuggc ucuugcccac      480

augaucaagu aucgcggaca uuuucugauu gaaggcgagu ucaauagcaa aaacaaugac      540

auccagaaga acuuucagga cuuccucgau accuacaaug cgauuuuuga auccgaccug      600

agccuggaga acucuaaaca gcucgaagag aucguuaagg auaaaauuuc caagcuggag      660

aagaaagaca gaauccuuaa gcuguucccc ggcgaaaaga auagcgggau cuuuucugag      720

uuccucaagc ugauuguggg aaaccaagcc gauuucaaaa aguauuuuaa ucuugacgaa      780

aaggcuuccc ugcacuucag caaagagucu uacgaugaag accucgagac acugcugggc      840

uauaucgggg augacuacuc cgaugucuuu cucaaggcca aaaagcugua ugacgcuauu      900

cuucugagcg gaauccucac cgugacagac aacggcaccg aggccccucu guccucugcg      960

augauuaugc gguacaaaga acaugaggaa gaucuugggc ugcucaaggc cuacauccgc     1020

aauaucagcc uggagacgua uaacgaaguu uucaaggaca acuccaaaaa uggauacgcu     1080

ggcuauauug auggcaagac aaaccaggag gacuuuuacg uguaucugaa aaaucuccug     1140

agcaacuucg agggggccga uuacuuucuu gaaaagaucg acagagagga uuuccugcgg     1200

aaacagagaa ccuuugacaa uggaucuauu cccuaucaga uccaccucca agaaaugcgc     1260

gcuauuaucg auaagcaggc caaguucuac ccuuuucugg ccaacaauaa agagcggauc     1320

gaaaagauuc ugacauucag aaucccguac uaugucggcc cccuugcucg cgggaacucc     1380

gacuuugcgu ggagcauucg gaaaagaaau gagaagauca ccccuuggaa cuucgaggau     1440

gugauugaca aagaaucuag cgccgaggcu uucaucaauc gcaugaccuc cuuugaucuc     1500

uaccugcccg aagagaaggu gcugccuaaa cacucucucc uguaugagac auucaacguc     1560

uacaaugaac uuacgaaggu ucgguuuauu gccgagagca uguccaagua ucaguuccug     1620

gacagcaaac agaagaaaga caucgugaga cucuacuuua aggauaaacg caaggugacc     1680

gacaaggaua ucauugaaua ucugcaugcc aucgacggau acgauggcau ugagcugaaa     1740

gggaucgaaa agcaauucaa cucuucccuu agcacauacc acgaccuccu gaauauuauc     1800

aacgauaaag aguuucugga cgauucuucc aacgaggcua ucauugaaga gaucauucau     1860

acccucacaa ucuucgaaga ccgggagaug auuaagcaga gacugagcaa auuugaaaau     1920

aucuucgaua aguccguccu uaagaaacug ucucgccggc acuauaccgg auggggcaag     1980

cucagcgcca aacugaucaa cggcauuaga gacgagaagu ccgggaauac gauccuggau     2040

uaccuuauug acgacggaau cucuaaccgg aauuuuaugc agcucauuca cgaugacgcg     2100

cugagcuuca aaaagaagau ccaaaaagcu cagaucauug gcgaugagga caaggggaac     2160

aucaaagaag ugguuaaguc ccugcccgga agcccggcca uuaaaaaggg cauccuccag     2220

ucuauuaaaa ucguggauga gcuggucaag gugaugggcg ggcgcaagcc ugaauccauu     2280

guuguggaga uggccagaga aaaucaguau acaaaccaag gaaaaagcaa uucucagcag     2340

cggcuuaaga gacuggagaa aucccucaag gagcugggca gcaaaauccu gaaggaaaac     2400

auccccgcua agcucucuaa aauugacaau aacgcccucc agaaugaucg ccuguaccug     2460

uauuaccucc aaaacgggaa ggacauguau accggagaug accuggauau cgaccggcuu     2520

agcaauuacg acauugauca uaucauuccu caggcuuuuc ugaaagacaa cuccaucgau     2580

aacaaggucc ucgugagcuc ugccuccaau agaggcaaaa gcgacgaugu ccccucccug     2640

gaggugguua agaagcgcaa aacccugugg uaccagcuuc ugaagucuaa acucaucagc     2700

cagagaaagu ucgacaaccu gacaaaagcg gaaagagggg gacucucccg cgaggauaag     2760

gccucuuuca uucaaagaca gcugguggag acccggcaga ucacaaagca cgucgcucgc     2820

cuucuggacg aaaaauuuaa uaacaagaaa gaugagaaua acagagccgu gcggacgguu     2880

aagauuauca cccucaaaag cacacuggug ucccaguucc gcaaggacuu ugaacuguau     2940

aaggucagag agauuaauga ucuucaucac gcucaugacg ccuaccugaa cgcgguggug     3000

gccucugcuc uccugaaaaa guaucccaaa cucgaaccug aguucguuua cggcgauuau     3060

ccgaaguaca auagcuuucg ggagcgcaaa uccgccaccg aaaaggucua uuucuacagc     3120

aacaucauga auaucuuuaa aaagucuauu ucccuggcug acgggacagu gaucgagaag     3180

ccccuuauug aagugaacga ggaaaccggc gagagcgucu ggaauaaaga gucugaccug     3240

gccacgguga gacggguucu cuccuacccu caagugaacg ucgugaagaa aguugaaaag     3300

cagaaucacg gacuggauag aggcaaaccc aaggggcugu ucaacgccaa ccuuagcucu     3360

aagccuaaac ccaauuccaa cgagaaucug gugggagcua aggaauaucu cgacccgaaa     3420

aaguacggcg gguaugcggg aaucagcaac uccuuugccg uccuggugaa aggcaccauu     3480

gagaaggggg cuaagaaaaa gaucacaaau guucucgagu uccagggcau uagcauccug     3540

gaucgcauca acuaccggaa agacaaacuu aauuuucugc uggaaaaagg auauaaggau     3600

auugagcuca ucauugaacu gccuaaguac ucucuuuucg agcugagcga cggcuccaga     3660

cgcaugcucg ccucuauccu gagcaccaac aauaaaagag gggaaauuca caagggaaac     3720

caaaucuucc ucucccagaa auuugugaag cugcuuuauc augccaaacg gaucucuaau     3780

acaauuaacg agaaucaccg caaguacguc gagaaccaua aaaaggaguu cgaagaacug     3840

uuuuacuaua uccuggaguu caaugaaaac uacgugggcg cuaagaaaaa ugggaagcuc     3900

cugaacagcg ccuuucaguc cuggcagaac cacucuauug augagcuuug cagcuccuuc     3960

aucggaccca ccggcagcga gcggaaaggg cuguuugaac ucacgucuag aggcuccgcg     4020

gcugacuucg aguuucuggg agugaagauu ccucgcuauc gggauuacac acccagcucu     4080

cuccugaaag acgccacccu uauccaccaa uccgucacag gccuguauga aaccagaauc     4140

gaucuggcca agcucgggga gggauga                                         4167


<210>  4391
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4391
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

aucacggaua auuauaaagu uccuucuaag aaaaugaagg ugcucggcaa caccagcaaa      120

aaguacauua agaaaaaucu gcuuggggug cugcuguucg acuccggaau cacagcugag      180

ggcagacggc ugaagcgcac cgccagacgg cgcuauacaa gacggcgcaa ccggauucuu      240

uaccugcagg aaaucuuuuc uaccgagaug gcgacacucg augacgcuuu cuuucaaaga      300

cuggaugaca gcuuccuggu cccggaugac aaacgcgauu ccaaguaucc caucuuuggg      360

aaucucgugg aagagaaagu uuaccacgac gaguucccua ccauuuauca ucugagaaag      420

uaccuugccg auucuacgaa gaaagccgac cugcggcucg uguaccuggc ucuugcccac      480

augaucaagu aucgcggaca uuuucugauu gagggcgagu ucaacagcaa aaauaacgau      540

auccagaaga auuuucagga cuuccucgac accuacaacg cuauuuuuga auccgaucug      600

agccuggaaa auucuaaaca gcucgaagag aucgucaagg acaaaauuuc caagcuggag      660

aagaaagaua gaauccuuaa gcuguucccc ggcgaaaaga acagcgggau cuuuucugag      720

uuccucaaac ugauuguggg aaaucaagcc gacuuccgga aaugcuuuaa ccuugaugaa      780

aaggcguccc ugcacuucag caaggagucu uaugacgaag aucucgagac acugcugggc      840

uacaucgggg acgauuauuc cgacguuuuu cucaaagcca agaaacugua cgaugcuauu      900

cuucugagcg gauuccucac cgugacagac aaugagaccg aagccccucu guccucugcu      960

augaucaagc gcuauaacga gcauaaagaa gaccuugccc ugcucaagga guacauuaga     1020

aacaucagcc ugaagacgua caaugaaguc uuuaaagaug acacaaagaa cggcuaugcg     1080

ggguacaucg auggaaaaac caaucaggag gacuucuaug uguaccugaa gaaccuccug     1140

gccgaguuug aaggcgcuga uuauuuccuu gagaaaauug accgggaaga uuuucugaga     1200

aagcagcgca cauucgacaa uggcuccauc cccuaccaga uucaccucca agagaugcgg     1260

gccauccugg auaagcaggc uaaauuuuau ccuuuccugg ccaagaacaa agaaagaauu     1320

gagaagaucc uuaccuuucg caucccguac uacguggggc cccucgcccg gggaaauagc     1380

gacuucgcuu ggucuauuag aaaacgcaac gagaagauca ccccuuggaa uuucgaagau     1440

gucauugaca aagaguccag cgcggaagcc uuuaucaacc ggaugacauc uuucgaucug     1500

uaucugcccg aggagaaggu ucucccuaag cacagccugc uuuacgaaac guuuaaugug     1560

uauaacgagc ugaccaaagu gagauucauu gcugaaucca ugcgcgacua ccaguuucuc     1620

gacucuaagc agaaaaagga uaucguccgg cuguauuuca aagacaagag aaaggugaca     1680

gauaaagaca uuaucgagua ccugcaugcc aucuauggcu acgaugggau ugaacuuaag     1740

ggaaucgaga aacaauuuaa uagcucccuc agcaccuacc acgaccugcu gaacauuauc     1800

aaugauaagg aguuccucga cgauucuucc aacgaagcca uuaucgagga aaucauucau     1860

acacugacca ucuuugagga ccgcgaaaug auuaaacagc ggcuuagcaa guucgagaac     1920

aucuuugaua agucuguucu gaaaaagcuc uccagacggc acuauacggg cugggggaaa     1980

cugagcgcua agcugauuaa uggaauccgc gacgagaaau ccggcaacac aauccuugau     2040

uaccucauug acgacggcau cucuaauaga aacuucaugc agcugauuca cgaugacgcc     2100

cugagcuuua agaagaaaau ccaaaaggcg cagauuaucg gggaugaaga caaaggaaau     2160

aucaaggagg uggucaaauc ccuccccggc ucuccggcua uuaagaaagg gauccugcag     2220

agcauuaaga ucguggauga acuuguuaag gugaugggag gccggaaacc ugaguccauu     2280

gucguggaaa uggccagaga gaaccaguau accaaucaag gcaagagcaa cucucagcag     2340

cgccugaaac ggcucgagaa gucccugaaa gaacugggga gcaagauccu uaaggagaau     2400

auucccgcca aacucucuaa gaucgacaac aaugcucugc agaacgauag acuguaccuc     2460

uauuaccugc aaaauggaaa agacauguau accggcgaug accuugauau cgaccgccug     2520

uccaacuacg acauugauca uaucauuccu caggccuucc ucaaggacaa uagcaucgau     2580

aacaaagucc uggugucuag cgcuuccaac agaggaaaga gcgacgauuu ccccucucug     2640

gaaguuguga agaaacggaa gacauuuugg uaccagcuuc ugaaauccaa gcucauuagc     2700

cagcgcaaau ucgacaaucu gaccaaggcc gagagagggg gccuccugcc cgaggauaag     2760

gcgggguuua uccaacggca gcuugucgaa acacgccaga ucacgaaaca cguggccaga     2820

cugcucgacg agaaguucaa caauaaaaag gaugaaaaca aucgggcugu ucgcaccgug     2880

aaaauuauca cacugaaguc cacccugguc ucucaguuua gaaaggacuu cgagcuuuau     2940

aaagugcggg aaauuaacga uuuucaucac gcccaugacg cuuaccugaa ugccgugauc     3000

gcgagcgccc uccugaagaa auauccuaag cucgagccgg aguucguuua cggagauuau     3060

cccaaauaca acuccuuucg cgaaagaaag ucugcuacag agaaagucua uuucuacagc     3120

aauauuauga acaucuuuaa gaaguccauc ucucuggccg acggccgggu gauugaaaga     3180

ccucuuaucg aggugaauga agagaccggg gagagcgucu ggaacaaaga auccgaccug     3240

gcuacggugc gccggguucu cagcuacccc caagugaaug ucgugaagaa aguugaggaa     3300

cagaaccacg gccuggauag aggaaagccu aaaggccugu ucaaugccaa ccuuucuucc     3360

aagcccaagc cgaacagcaa ugagaaccug gugggggcca aagaauaucu cgacccuaag     3420

aaauacggag gcuaugcugg gauuucuaau uccuucgcgg uccuggugaa gggaaccauc     3480

gagaaaggcg ccaagaagaa aauuacaaac guucucgagu uucaggggau cagcauucug     3540

gaucgcauca auuaccggaa ggacaaacuu aacuuccugc uggaaaaggg cuauaaagau     3600

aucgagcuca uuaucgaacu gcccaaguac ucucuuuuug agcuguccga cggaagcaga     3660

cgcaugcucg cuuccauucu gagcaccaau aacaagcggg gcgagaucca caaagggaau     3720

caaauuuucc ucucucagaa guuugugaaa cugcuuuauc augccaagag aaucagcaac     3780

acaaucaaug aaaaccaccg caaauacguc gagaaucaua agaaagaguu cgaagaacug     3840

uuuuacuaua uucuggaguu caacgagaau uacgugggag ccaagaagaa cggcaaacuc     3900

cucaauuccg cuuuucaguc uuggcagaac cacagcaucg augaacuuug uuccucuuuc     3960

auugggccua ccggaagcga gcggaagggc cuguuugaac ucacguccag agggucugcc     4020

gcggacuucg aguuucuggg cgugaaaauc ccccgguauc gcgauuacac accuagcucc     4080

cuccugaagg acgcuacccu uauucaccaa agcgucacag gacuguauga aaccagaauc     4140

gaucuggcca aacucggcga gggguga                                         4167


<210>  4392
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4392
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

aucacggaua auuauaaagu uccuucuaag aaaaugaagg ugcucggcaa caccagcaaa      120

aaguacauua agaaaaaucu gcuuggggug cuucucuucg acuccggaau cacagcugag      180

ggcagacggc ugaagcgcac cgccagacgg cgcuauacaa gacggcgcaa ccggauucuu      240

uaccugcagg aaaucuuuuc uaccgagaug gcgacacucg augacgcuuu cuuucaaaga      300

cuggaugaca gcuuccuggu cccggaugac aaacgcgauu ccaaguaucc caucuuuggg      360

aaucucgugg aagagaaagc cuaccacgac gaguucccua ccauuuauca ucugagaaag      420

uaccuugccg auucuacgaa gaaagcugac cugcggcucg uuuaccuggc ccuugcucac      480

augaucaagu aucgcggaca uuuucugauu gagggcgagu ucaacagcaa aaauaacgau      540

auccagaaga auuuucagga cuuccucgac accuacaacg ccauuuuuga auccgaucug      600

agccuggaaa auucuaaaca gcucgaagag aucgugaagg acaaaauuuc caagcuggag      660

aagaaagaua gaauccuuaa gcuguucccc ggcgaaaaga acagcgggau cuuuucugag      720

uuccucaaac ugauugucgg aaaucaagcg gacuuccgga aaugcuuuaa ccuugaugaa      780

aaggccuccc ugcacuucag caaggagucu uaugacgaag aucucgagac acugcugggc      840

uacaucgggg acgauuauuc cgacguguuu auuaaagcua agaaacucua cgaugccauc      900

cugcuuagcg gauuccugac cguuacagac aaugagaccg aagcuccucu cuccucugcc      960

augauuaagc gcuauaacga gcauaaagaa gaccuggcgc uucugaagga guacaucaga     1020

aacaucagcc ucaagacgua caaugaagug uuuaaagaug acacaaagaa cggcuaugcc     1080

ggguacauug auggaaaaac caaucaggag gacuucuaug ucuaccugaa gaaccugcuc     1140

gcugaguuug aaggcgccga uuauuuccug gagaaaaucg accgggaaga uuuucuuaga     1200

aagcagcgca cauucgacaa uggcuccauu cccuaccaga uccaucugca agagaugcgg     1260

gcuauucucg auaagcaggc caaauuuuau ccuuuccugg ccaagaacaa agaaagaauc     1320

gagaagaucc ugaccuuucg cauuccguac uacguggggc cccuugcucg gggaaauagc     1380

gacuucgcgu ggucuaucag aaaacgcaac gagaagauua ccccuuggaa uuucgaagau     1440

gugaucgaca aagaguccag cgccgaggcu uuuauuaacc ggaugacauc uuucgaucuc     1500

uaucugcccg aagagaaggu ccugccuaag cacagccucc uguacgaaac guuuaauguu     1560

uauaacgagc uuaccaaagu gagauucauc gccgaaucca ugcgcgacua ccaguuucug     1620

gacucuaagc agaaaaagga uauugugcgg cucuauuuca aagacaagag aaaggucaca     1680

gauaaagaca ucaucgagua ccugcacgcc auuuauggcu acgaugggau cgaacugaag     1740

ggaauugaga aacaauuuaa uagcucccuu agcaccuacc acgaccuccu gaacaucauu     1800

aaugauaagg aguuccugga cgauucuucc aacgaagcua ucaucgagga aauuauccau     1860

acacucacca uuuuugagga ccgcgaaaug aucaaacagc ggcugagcaa guucgagaac     1920

auuuuugaua agucugugcu uaaaaagcug uccagacggc acuauacggg cugggggaaa     1980

cucagcgcca agcugaucaa uggaauccgc gacgagaaau ccggcaacac aauucuggau     2040

uaccuuaucg acgacggcau uucuaauaga aacuucaugc agcucaucca cgaugacgcg     2100

cugagcuuua agaagaaaau ucaaaaggcu cagaucaucg gggaugaaga caaaggaaau     2160

auuaaggagg uugugaaauc ccugcccggc ucuccggcca ucaagaaagg gauucuccag     2220

agcaucaaga uugucgauga acuggugaag guuaugggag gccggaaacc ugaguccauc     2280

guggucgaaa uggccagaga gaaccaguau accaaucaag gcaagagcaa cucucagcag     2340

cgccuuaaac ggcuggagaa gucccucaaa gaacugggga gcaagauucu gaaggagaau     2400

auccccgcua aacuuucuaa gaucgacaac aaugcccucc agaacgauag acuguaccug     2460

uauuaccucc aaaauggaaa agacauguau accggcgaug accuggauau ugaccgccuu     2520

uccaacuacg acaucgauca uauuaucccu caggcuuucc ugaaggacaa uagcauugau     2580

aacaaagugc ucgucucuag cgccuccaac agaggaaaga gcgacgauuu ccccucucug     2640

gaagugguua agaaacggaa gacauuuugg uaccagcugc uuaaauccaa gcugaucagc     2700

cagcgcaaau ucgacaaucu caccaaggcg gagagagggg gccugcuccc cgaggauaag     2760

gccggguuua uccaacggca gcugguggaa acacgccaga uuacgaaaca cgucgcuaga     2820

cuucuggacg agaaguucaa caauaaaaag gaugaaaaca aucgggccgu gcgcaccguu     2880

aaaaucauua cacucaaguc cacccuggug ucucaguuua gaaaggacuu cgagcuguau     2940

aaaguccggg aaaucaacga uuuucaucac gcucaugacg ccuaccuuaa ugcggugauu     3000

gccagcgcuc ugcucaagaa auauccuaag cuggagccgg aguucgugua cggagauuau     3060

cccaaauaca acuccuuucg cgaaagaaag ucugccacag agaaaguuua uuucuacagc     3120

aauaucauga acaucuuuaa gaaguccauu ucucucgcug acggccgggu caucgaaaga     3180

ccucugauug aggugaauga agagaccggg gagagcgugu ggaacaaaga auccgaccuu     3240

gccacggucc gccgggugcu gagcuacccc caaguuaaug uggucaagaa aguggaggaa     3300

cagaaccacg gccucgauag aggaaagccu aaaggccugu ucaaugccaa ccugucuucc     3360

aagcccaagc cgaacagcaa ugagaaccuu guuggggcua aagaauaucu ggacccuaag     3420

aaauacggag gcuaugcggg gaucucuaau uccuucgccg ugcucgucaa gggaaccauu     3480

gagaaaggcg cuaagaagaa aaucacaaac gugcuggagu uucaggggau uagcauccuc     3540

gaucgcauca auuaccggaa ggacaaacug aacuuccuuc uggaaaaggg cuauaaagau     3600

auugagcuga ucauugaacu ccccaaguac ucucuguuug agcuuuccga cggaagcaga     3660

cgcaugcugg ccuccauccu cagcaccaau aacaagcggg gcgagauuca caaagggaau     3720

caaaucuucc ugucucagaa guuuguuaaa cuccuguauc augccaagag aaucagcaac     3780

acaauuaaug aaaaccaccg caaauacgug gagaaucaua agaaagaguu cgaagaacug     3840

uuuuacuaua uccuggaguu caacgagaau uacgucggag cuaagaagaa cggcaaacug     3900

cucaauuccg ccuuucaguc uuggcagaac cacagcauug augaacugug uuccucuuuc     3960

aucgggccua ccggaagcga gcggaagggc cuuuuugaac ugacguccag agggucugcg     4020

gcugacuucg aguuucucgg cgugaaaauu caacgguauc gcgauuacac acccagcucc     4080

cugcucaagg acgccacccu gauccaccag agcgugacag gacuuuauga aaccagaauc     4140

gaucuggcca aacugggcga gggguga                                         4167


<210>  4393
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4393
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acgagaaaga aaucuccaag auuaucaaac ugggcguccg gguuaauccc      120

cucacaauug augagaagac gaacuucgaa aaagggcgcc cucugucuac caaugccgac      180

agaacacuua agcggggagc gcgcagaaac cugcagcggu acaagcuccg cagagagaau      240

cugaucgaga uccugauuga uaaccacauc auuaccaaag aaacaccgcu caccgagauc      300

ggcaagggga caaccuauca aacgcugagc cuucgggcua aagccgugcg cgaaagaauu      360

gaccuggagg auuuugccaa ggugcuccug gcuaucaaua aaaagcgggg auacaagucc      420

ucucgcaaag ccagcaacga cgaggaaggc caggcuauug auggcauggc caucgcgaag      480

gagcuuuaug aaagagggau cacccccgga caguacgucc uggagcuccu ggaaaaugac      540

cggaaauaua uuccugauuu cuaccgcucc gaccugaagc aggaguuuaa caaaaucugg      600

aauaaccaaa agacauucua ucccgagauu cucaccgaug aacuguuuaa agagcuugac      660

ggcaagaaua agucucagac augggccauc ugccagaaac cuuucaacau uaaggggauc      720

aaaagaauca ccaagggacg ggaaaagacg auugagaacu accauuggag agugaagggc      780

cugagcggga agcucaaucu ggaaaaacuu gcuaucauuc ugcaggagau caacaaugau      840

auuaaccaau ccagcggaua ccucggcggc aucucugaca aguccaaaaa gcuguaucug      900

aauaaagaga caguugggga auuucucuac aagcagaucg ccaagaaccc ccacaccagc      960

cugaaaaauc agguguucua ucgccaugac uaccuugaug aguuugaaca gauuuggaag     1020

acacaaucua acuucaaugc uaaccugacc gacgagcuca aacaggaaau ccgggauguc     1080

auuaucuuuu aucagagaaa gauuaaaucc caaaagagcc ugaucucuuu cugucaguuu     1140

gaguccuggg agcaggaaua cuucgacaag gagaccggaa aaacaaagac gcgcaccgug     1200

ggccggagag uuauuccuaa aagcuccccg cuuuuccagg aauuuaagau cuggcaaaau     1260

cugaaccacc ucguguucac aaauucugag accgaugaga aaaucgaaau uaaggagguc     1320

gaacaggaga uccgcaaaga acuguuugag gagcugaacc uccggggaga ccugaauccc     1380

aacgaucuuc ugaagauucu cagaagccag uucaagaucg gaaaacuguc caacuggaag     1440

ugcaauuuug agaaaauuga gggcaaccgc acaaaugccg cgcuguucaa caucuaucag     1500

aagaucagcg acuuugaagg guacggaauu gauuggaaua aaucuaacgc caagaagauc     1560

cggaaagagc uuaaggcuac cuucccugaa auuggcaucg acgccuccau ucucgaguuu     1620

gaugcuuaca aaaagcaacu gacgaaucag agcucuuaug agcuguggca ucuccuguac     1680

agcgccgaag aggacuucaa aaucuccgaa caggacaaac uuaucuaugg caacucuagc     1740

gugucccuga agaaaaagcu ccaccugaaa uuuggguuca aucccgagua cgccaagaug     1800

cuggcuaacc uuagccucau gucugauuau ggaaaucugu ccgcgaaagc cauuaagaag     1860

auccugccuu accucgaaga cggccaccug uauagcgagg cuuguagacu ugccggguac     1920

aaccauucua aaucccugac agccgaggaa cucgagaagu uugaacccaa agcuaagcug     1980

gaucugcuua aaaagaauag ccuccgcaac ccgguggucg agaaaauucu gaaucagaug     2040

guuaacgugg ugaaccaagu caucgacacc uacggaaagc cugauggcgu gcggauugag     2100

cuggccagag aacucaagaa auccgcgaag gagcgggcug aaaugaccaa agggaucgcc     2160

gacggcacac gcagaaauga ggaaaucaag aaaauuauca cccagaaguu cgggauuccc     2220

aacccuacaa agucugaucu gguucgguau agacuuuggc aggagcugga gagccgcggc     2280

uacaaagaca ucuucacgga uaaguauauu ucccccgaca aaaucuuuuc uagcgaaauu     2340

gauaucgagc acaucauucc caaggcccuc cuguucgacg auuccuuuag caauaaaacc     2400

cugucuuucc ggaacgugaa ucuuaagaag gcuaacagaa cagcccucga cuuuaucacc     2460

caggacuacc aauccaaugu cgaggcuuau aaagagcgcg uggaaacaug gaacaaggau     2520

aaucauauua gcaaagggaa gagaaacaaa cugcugaugu cucucaagga caucccugau     2580

ggauucauug agcgggaccu gcgcaauucc caguacaucg ccagaaaggc gaaacggaug     2640

cuuuaugagg uuuuuccgaa cgaagugcug gccaccagcg gcucuaucac ggauaagcuc     2700

cgcagcgacu gggaucugau uaaugucaug aaagagcuga accuucccaa guacaaagcu     2760

cuggggcuca ccgaauacga ggaaagacug gacaagggaa cagaggaacu cuucaagcac     2820

gaagugauca aagauuggac caagcggaau gaccaccgcc aucacgccau ggaugcucug     2880

gccgucgcgu uuacaaccca uaaccacauu caguauauca acaaucuuaa cgccagaaaa     2940

gacauuacgc aagauaauca uccuaucauc aagaacauug agaaucugau cacagaagug     3000

aacuccaaag gcaagcgggu uuucaaagcu cccaugagcu cuuuucgcga guccgccaag     3060

gagcagauug aagcuauccu cgugagcuuc aagaauaaaa acaaggucgu gaccaaaaau     3120

auuaaccaga caaagaaaga agggggcuuu aauuaccaga acgccaagac cccuagagga     3180

caacugcaca aggaaaccau cuauggcaaa uccaagcagc cccuggagaa accgguuaag     3240

auuaauaacc gguucugcaa aaagcaagug gggcuuauca ucaauuucaa ggaacaggag     3300

gccguccugc accaucucaa aaaguacgac aacaaugaga aaauugcuuu uucuaacaag     3360

acacugaaaa gcgacccuau ccaggugaac ggaucucccc ucagagaagu gaagauuuuc     3420

gaggaaaucu uuacgauucg caaggauauc aauccugaca acuucaaaac ccagaagcac     3480

cuggauaaaa ucauugacaa gggcaucaaa gagguuauga agaaacggcu ugaagaguau     3540

aauggggauu uuaagacagc guucuccgac cuggauaaga accccauuug gcucaaucag     3600

agcaaaggaa ucaccauuaa gagagccaca aucuccggcg ucaccaacgc ugagccucug     3660

caucaggcca aagaccaccu ggggaauauc auucuugaug acaacggcaa ggaaaucccc     3720

auugauuacg ugucuacggg aaauaaccac cauguggcca ucuaucaaga cgagagcggc     3780

aaucugcagg aagaggucgu guccuuuuac gaagcuguua uucgcaaaaa ccaggaccaa     3840

ccgaucauua agaagcggca cccuaaaggg uggaaguucc ucuuuacaau gaaacagaau     3900

gagauguucg uguuuagcuc ugagaacuuc aaucccuccg aaaucgaucu gcucgacgag     3960

aagaacgccg cgaucauuag ccccaaccug uucagagucc agaaaaucgc uaccaagaau     4020

uacauguuuc gccaucaccu ugaaaccgag gugacaaaca aucuggauuu caccuaucgg     4080

cauuuucagg ccacagagug gcugagaaac cucuguaagg uucgcauuga ccacaucgga     4140

cggauugugc aaaucggcga auacuga                                         4167


<210>  4394
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4394
augaucauua gaaccuacga gcugaagaca aucacgacca uugccauccu caaaauguuc       60

cagcugcuua caggcgggcu gagcaugacc aagcucggaa aacccuaugg cauuggggug      120

gacaucggau ccaacucuau cggcuuugcu gccgucgaug aaaauagcca ccugauucgg      180

cuuaagggga aaacaguuau cggagcgcgc cuguucgagg aaggcaaggc ugccgccgac      240

agacgggcuu cccgcaccac aagacggcgc cucucuagaa accgguggcg ccugagcuuu      300

cugagagauu ucuuugaguc ccauauuacc ccuacgggcc cgaauuucuu uaugcggcaa      360

aaguacucug aaaucagccc caaagacaag gcccgcuaua aauacgagaa gagacucuuc      420

aacgaucgga ccgacgcuga guuuuaucag caguacccua caauguauca ucugcgcaau      480

agacuucuga ccgaucccuc caaagccgac gugcgggaaa uuuacuucgc gauccaccac      540

auucucaaga gcagagggca uuuucugaca ucuggauacg ccaaggauuu caacaccaau      600

aaaguggcuc uuaacgagau cuuuccugcc cugcaggacg cuuaugccca agucuacccc      660

gaucucgaca ucacguucga ugaaaauaag gugaacgagu ucaaaacagu ucugcugaau      720

gagaaggcga ccccuuccga cacacagcgc gcccucguga accugcuucu ggcugaagac      780

ggcgauaaag acauucucaa gcagcagaaa caaguccuga ccgaguuugc caaggcugug      840

guugggcuua agaccaaacu gaauguggcc cucggaacag aagucgauag cucugaggcc      900

acggugugga acuucucccu gggccagcug gacgauaagu gggcugggau cgaaagcgcg      960

augaccgacg agggaacaga gauucucgau cagauccggg accuguauag agcccgccuu     1020

cugaauggca uugugccggc uggcaaaacc cucucucagg ccaaggucga ugacuacgcc     1080

caacaccacg aagaucugga gcuuuuuaaa gacuaucuga agcagcucga agacgauggg     1140

acagcuaagg ccauccggca gcuguacgac agauauaucg auggagacga ugcgaaaccc     1200

uucguucgcg aggacuuugu gaaggcucug accaaagaag ugacggccca uccuaacgcc     1260

aagucccccg agcuccugga gcggcuugcu caaccugauu ucaugcugaa acagagaaac     1320

aaggccaaug gcgcuauucc cguccagaug cagcaacgcg aacucgacca gaucauuaag     1380

aaccagagcg uguacuacga uuggcuggcc gcgccgaauc cuguugagaa acaccggaag     1440

uccaugcccu aucagcugga cgaacuucuc aacuuuagaa ucccuuacua uguggggccc     1500

cuggucacag ccaaagagca aaaggcugcc cagggaggcg uguucgcuug gaugguucgc     1560

aaagaucccg acgggaauau uaccccuuac aacuuugaug aaaaggugga ccgggaggcc     1620

ucugcgaaua ccuucaucca gagaaugaag acaaccgaca cauaucugau cggagaggau     1680

guccucccga aacagagccu gcuuuaccaa cgguaugaag ugcugaacga gcucaauaac     1740

guccgcguga auaacgaaaa gcuguccauu gagcagaaac agcagguuau cagagagcug     1800

uuugaacggc auaauagcgu gacgauuaag caauucgccg agaaccuuag agcucacggc     1860

gacuacgccc acauccccga aauucgcggc cucgcugaug agaaacgguu ucugucuucc     1920

cugagcaccu acagacagcu caagucucug cuuccugacg ccaucgauga ccccgccaaa     1980

caggcugaua uugaaaauau caucgcgugg agcacagucu ucgaggacgc cgcuauuuuc     2040

aagaccaagc ugaaagagau caacuggcuc gauucccaaa caauuaccaa gcugucuaac     2100

auccgcuaua gagggugggg acaguuuagc cauaaauucc ugaauggccu uacgcucggg     2160

aacggacaca ccauuaucca ggaacuguuu cuguccaaua acaaucucau gcagauccug     2220

acagacgaga cccuucaaaa gaaaaugaca gaacugaacg ccgauaagcu caaggccgcu     2280

aauauuaacg acgccaucga ugacgcguac accagcccuu cuaauaaaaa ggcucugcgc     2340

caggugcugc ggguuauuga ggacaucaaa agagccgccg auggccagga ccccuccugg     2400

cuuuaugugg aaacggcuga ugggggcgga acaccgggca agcggacccg cgccagacag     2460

caucaacucc aggagauuua cgcgaacgcu gcccacgagc ugaucgacag cgccguccgc     2520

ggggaacugg aggauaaaau cgcugacaag gccgauuuca augaccggcu cgugcuguau     2580

uuuaugcagg gaggcagaga uauuuacaca gggaccccuc uuaacaucga ccagcugucu     2640

uccuaugaua uugaccauau ccucccccaa agccugauua aggacaauuc ccuggauaac     2700

cgggugcuug uucacgcuau caucaaucgc gagaaaaacg ccaccuucgc gucuacaauu     2760

uacgcccaga agaugaacgc uacguggaga caguggcacg aggccggacu gaucagcggc     2820

cggaaacuca gaaaucugca aaugcgcccu gaccagauug auaaguacgc uuccggguuu     2880

gucgcccggc agcucgugga aaccagacag aucauuaaac ugacagagca aaucguggcc     2940

gcucaguauc ccgagaccaa gauuaucgcg gucaaggccg gccuuucuca ucagcugcgc     3000

gaagaacucg aguucccuaa aaaccgggac gugaaucacu accaucacgc uuuugaugcc     3060

uuccuggccg cuagaaucgg aacauaucug cuuaagcgcu uucccaaccu gcaggccuuc     3120

uuuaccuacg gcaaauucaa gaaagcggac guuaagaaac uccggggguu caauuuuauu     3180

agagauauca cgcacgcuga agacaagauu guggucaagg auacaggaga ggugaucugg     3240

gacaaaaagc gcgauguuga cgaacuggau cggauuuaua acuucaaaag aaugcucauc     3300

acccaugagg ugcacuuuga aaaggccgac cuguucaaac aaaccgucua cggcgccaag     3360

gacagcaagg aggcuggggg caaaaagcag cuuaucucca aaaagaaagg auauccggug     3420

gauauuuacg ggggcuacac acaggaaacc ggaagcuauc ugucuguugu gcggcucaca     3480

aagaaggcca uguacgcggu cgugaaagug uccacgcgcg acgcugccaa gcuggccguc     3540

gcuaaaagca ucucugagca gaaggagaau gaaacccuga acgauauuau cgacgagaaa     3600

cuuuccaaga uuagcaagaa uggcaaaauc acacaugaac uguuugaggu ugugcucccu     3660

agaguggggc aaaagacccu guucaaaaac ucuaaguaua aucucuuucu ggucaacagc     3720

gauacauaca ugcacaauua ucaggaacuu uggaugcccc gggaguacca gaaaaugugg     3780

aaggacaucu cccugagcaa guauggagau gcccagaccg aggaccaacu ggaucagauu     3840

uucggcuuua ucgugucuca gguuaacucc uacuucaauc ucuacgacau uaaccaauuu     3900

cgcaaaaagc ugaacgaugc ugccgacaaa uucgcgacgc uucccaugag agauaccgac     3960

gacaugcagg ggaagaucgc cacaauugga cagcugcuca ucggccugca ggcuaaugcc     4020

gcucggagcg aucucagaaa ccuggacauu aaaaccccuc uugggcugcu gcaaaucccg     4080

aauggcaucu cccucgauuc ugacacaagc guggucuauc agucccccac cggacugcuu     4140

gaacgcgagg ugcaucuguc ugaucucuga                                      4170


<210>  4395
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4395
auggccagag acuacagcgu gggccuggau aucgggaccu ccucugucgg augggcugcc       60

auugacaaca aguaucaccu cauccgggcg aaaagcaaga aucugauugg cguucgccuu      120

uucgauuccg cugugacagc cgagaaaaga cggggguacc gcacgaccag acggcgccug      180

ucuagacggc auuggcgccu cagacugcuu aacgacaucu uugccggacc ccugacagau      240

uucggcgacg aaaauuuucu cgcucggcug aaguauagcu gggugcaccc ucaggaucaa      300

uccaaccagg cccauuucgc ugccgggcug cucuuugacu cuaaagagca ggauaaggac      360

uucuaccgca aguauccgac cauuuaccac cugagacuug cgcugaugaa ugaugaccag      420

aaacaugauc uccgggaagu cuaucuggcc auccaccacc uugugaagua ccgcggacau      480

uuucugaucg agggcgacgu uaaagcugac agcgccuucg augugcacac auuugcugac      540

gccauucaaa gauacgcgga auccaacaau agcgaugaga accuccuggg caagaucgac      600

gagaaaaagc ugucugccgc ucucaccgau aagcaugggu ccaaaagcca gcgggccgaa      660

acagcugaga ccgccuucga cauucuggau cuucagucua agaaacagau ccaagccauu      720

cugaaguccg ucgugggaaa ucaggcuaac cucauggcga ucuuuggccu ggacagcucu      780

gccauuucca aagaugaaca gaagaauuau aaauucagcu uugacgaugc ugacaucgac      840

gagaagaucg ccgauuccga ggcccuucug ucugacacgg aguucgaguu ccucugcgau      900

cugaaggcug ccuuugacgg gcugacccuc aaaaugcugc uuggagauga caagacaguu      960

agcgcggcua uggugagacg cuucaacgaa caccagaaag auugggagua cauuaagucc     1020

cacauccgga augccaaaaa cgccggcaau gggcuguaug aaaagagcaa gaaauuugac     1080

ggaauuaacg cugccuaccu cgcucugcaa ucugauaacg aggacgauag aaagaaagcc     1140

aagaaaaucu uccaggacga gauuuccagc gcggauaucc ccgacgacgu caaggccgau     1200

uuucuuaaga aaaucgacga ugaccaguuc cugccuauuc aacgcaccaa gaauaacggc     1260

acaauccccc aucagcucca ccggaaugaa cuggagcaga uuaucgaaaa acagggcauu     1320

uauuacccuu uucugaagga uaccuaucaa gagaacucuc augaacucaa uaaaaucacg     1380

gcucugauca acuucagagu gcccuacuac guggggccuc uugucgagga ggaacagaag     1440

auugccgacg auggaaagaa uaucccggac cccacaaacc acuggauggu ucgcaaaagc     1500

aaugauacca uuacaccuug gaaccugucc cagguggugg accucgauaa gucuggccgg     1560

agauuuaucg agcgccugac cgggaccgac acauaucuga uuggagaacc cacgcuuccu     1620

aaaaauagcc uccuguacca gaaguucgau guccugcaag agcucaacaa uauccgggug     1680

uccggcagac gccuggacau ucgggcuaaa caggacgccu uugaacaucu uuucaagguu     1740

cagaaaaccg ugagcgcgac aaaccugaag gauuuucucg uccaggccgg guauaucucu     1800

gaggacaccc aaaucgaggg acuggcugau gugaauggca agaacuucaa caaugcccug     1860

acaaccuaca acuaucuugu uuccgugcuc ggcagagagu ucgucgagaa ucccagcaac     1920

gaagaacugc uggaggaaau uacggagcuc cagacagugu uugaagacaa aaagguccug     1980

cggcgccagc uugaucagcu ggacgggcuc ucugaucaca auagagagaa acugucccgg     2040

aagcacuaca ccggaugggg cagaaucagc aaaaagcugc uuaccacaaa gauugugcaa     2100

aacgcugaca aaaucgauaa ucagaccuuc gacguuccgc gcaugaacca guccauuauc     2160

gauacacucu auaauacgaa gaugaaccug auggaaauca uuaauaacgc cgaggacgac     2220

uuuggggugc gggccuggau cgauaaacaa aauaccacag acggagauga gcaggacguc     2280

uacucucuga uugaugaacu cgcuggcccu aaggagauca aaagagggau ugugcagagc     2340

uuccgcaucc uggacgauau caccaaggcg guuggcuacg cccccaagag aguguaucuu     2400

gaauuugcuc ggaaaacaca ggagucccau cugaccaacu cucgcaagaa ucaacucagc     2460

acgcugcuga aaaacgccgg acuuuccgaa cucgucaccc aggugagcca guacgacgcc     2520

gcugcccugc agaacgauag acuguaucuc uacuuccugc aacagggcaa ggacauguau     2580

ucuggggaga aacuuaaucu ggauaaccuc uccaauuacg acauugauca caucauuccu     2640

caggcguaua caaaggacaa cagccuggac aaucgggugc ugguuucuaa caucaccaau     2700

cgcagaaagu ccgauagcuc uaacuaccuu cccgcucuga uugacaaaau gcggcccuuu     2760

uggagcgucc ucuccaagca gggacugcuc agcaaacaua aguucgccaa ucugacacgc     2820

accagagauu uugacgauau ggagaaagaa cgguucaucg cccgcucucu uguggagacg     2880

agacaaauca uuaagaacgu ggcuucccug aucgacagcc acuuuggcgg ggaaacaaaa     2940

gccgucgcga uucgguccuc ucucaccgcu gauaugagac gcuacgugga caucccuaag     3000

aaucgggaua uuaacgacua ucaccaugcc uucgaugccc ugcuguuuag cacaguugga     3060

caguacaccg aaaauuccgg ccuuaugaag aaagggcagc ugucugacag cgcuggcaac     3120

caauauaaua gauacaucaa ggaauggauu cacgccgcuc gccucaacgc ccagucccag     3180

cgggugaacc cguucggauu cgucgugggc ucuaugagaa augcggcccc cgggaaacug     3240

aacccugaga ccggagagau cacacccgaa gagaaugcug auuggagcau cgccgaccuc     3300

gacuaucugc auaagguuau gaacuuucgc aaaauuacgg ugacccggag acuuaaggau     3360

cagaagggcc aacuguacga cgaaucccgc uauccuagcg uccuccacga ugcuaaaucu     3420

aaggccucca ucaauuucga caaacauaag cccguggauc uguacggggg auuuagcucu     3480

gccaaaccgg cuuacgcggc ccugauuaag uucaagaaca aauuucggcu uguuaaugug     3540

cugagacagu ggacauauuc cgacaagaac agcgaggauu acauccucga gcagauucgg     3600

ggcaaauauc cuaaggcuga aaugguccug ucucacaucc ccuacgggca gcucgugaaa     3660

aaggacggcg cccuggugac caucuccagc gccacagagc uucacaauuu cgaacaacug     3720

uggcugccuc ucgcugauua uaagcugauu aacacccuuc ugaaaacgaa ggaggacaau     3780

cucgucgaua uccugcauaa ccgccucgac cugcccgaaa ugacaauuga guccgccuuu     3840

uacaaagcgu ucgacagcau ccuuucuuuu gcuuucaaua gauaugcccu gcaccagaac     3900

gcccugguua agcuccaggc ucaucgggau gacuuuaaug cccugaacua cgaggauaaa     3960

cagcaaaccc uugaacgcau ucuggacgcg cuccacgcua gcccugccuc cucugaucug     4020

aagaagauca aucucagcuc cggauucggc agacuguucu cucccagcca uuuuacccuu     4080

gccgacacag augaguucau cuuucagucc gugaccgggc uguucucuac acagaaaacg     4140

guggcucagc uguaccaaga aaccaaguga                                      4170


<210>  4396
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4396
augaagaacu acaauaucag ccuggugccc uccccuucug gcgucgggua ugccguuacc       60

gacgauaacc ucaauauuau caaaccggug caccauguga gcggaauugg cuccagacug      120

uucaacgagg ggucuagcaa gcaggaccgg cgcaauucca gaucugcucg gcgcagcaga      180

caccggcaac agggacgcau caaagcccuu aacuccauua ugaaggauga aaucaucaaa      240

gucgacccca cauuuuucga uagacuguac cagagcgagc ucucuccucu ggacaagaau      300

aagaaauauc ggaacauuau cuuugauaag cccuccgugg aaagcuacua uuacaaaaag      360

uucccuacga uuuaucaucu ugagaaauac cugaucaaua ccgacggcaa ggcggauauu      420

cgccucaucu acugggcucu gcacucucug cucacacaua gagggcacuu uuauaacacc      480

acacccguuu cccaguucaa gccuggagcc cugaauauua aagacaaguu uaucgaacuu      540

aaccaacuga aucaggugaa aaccuucgag uuuagcgucg ccaacacacc ggagaucgaa      600

cacauucuca aggaucggua caucucuaaa cagaauaaga uuaaccagcu ggcugagcuu      660

cuguucgaac aagagcccga gaaagacauc cagaaggcca acaccaaggu ggcuaaaacg      720

auugccaagg cgauccucgg caauaaauuu cagcuggaug aaaucauugg ccuggacguu      780

aacaaguccg agaaaaagga auggagccuc cagcuguccg accuuaaucu ggauucuaag      840

aucgacgagc ucagcgauuc ccugaccgac caacagcuuc gcauucugga aauccuccag      900

gagauguaug ccgcuacaac ccugcuggau auucuccaag ggagcgacuc ucugcuugau      960

gccaaaauca aguccuacaa cgaguauaaa cagaauagac uccuguucuu ugaccugcuu     1020

aagacacugc cugauaaaaa ggcucaucuc cugcggcagg gauacacccu guaucucaac     1080

aaucaccauc gcgaccuggc cagcgcgaga aaggaucuug gcaucguggc cgcuacgaac     1140

uucucuaaaa cagaccugua cgcccucauu aagaaaaccc ugaaggacga acccgagaca     1200

ccugaaagca aaaagguccu ggggcuuauc gauaagggag aguuccucaa uaaacagcgg     1260

accgaccuga acaccuacau ucccuaucaa cugaaugcuc ucauccugaa caagauucuu     1320

aaaaaucagg gcaagcacua cccguuucug aucaaagaaa acccugccga gcccgauaga     1380

aaggaggccc cuuaugaaau uucccagcuc augcaguuca caauccccua cuauugcggg     1440

ccccuggcua cgccugagga acaaaagaau cugccggagu cuagccgcuu uggauggcuu     1500

gugcggaagg aagagggcag aaucaccccc uggaacuucu acaacaagau uaaugugauc     1560

gagacagcga acgccuuuau uaaacgcucc aucggcaagg acaccuaucu ccugagcgaa     1620

ccuguccugc ccgauaauuc ucuccuguac cagaaauaca agguucuuaa cgagcugucc     1680

agcauuucuc ucguggacgc uaauaccaaa aaggaaaaga aacugacagc caagaucaaa     1740

cagcugcuuu auaaggaggg guucaaaaag cacgugacgg ucuccaagac aauggcccuc     1800

aaaauccuga acgauaauaa cgugccuacc agcgacauuc ugggacucuc caccggcaag     1860

aaauuugaua auucucugag cacauacaac uccuggaaga aggcuuuccc cgagcagauc     1920

gacaauccgc auuaucggca agaucuugaa uacaugauug aguggucuac cguuuuuaca     1980

gacagaaaga uccuggugga aaagcucaaa gagauugaau ggcugcgccc ugagcagauc     2040

aaguucgucc uggagcaccg gcuuacgggg uggggaaaac ucagcaagag acugcuggug     2100

gaacucaaag auaacaaggg caaauccaau aucgacaacc uguuuaauac caagaagaac     2160

uucaugcaga uuguuagcca gcaaguguau aacacacaga ucgaggccau ugcgcuuaaa     2220

accacaaaga aucagucucu ggacaacauc cucgaagcug ccuacaccuc ccccgccaau     2280

cgcaaagcua uucggcaaac gaugaagguc gugaacgaga ucgucacccu ggccgggaaa     2340

ggaacacauc cugauaagau cuuucugacc uuccagagaa gcaaggaaca ggagggcaaa     2400

cuuacaaccg accgcgcuuc uucccugcuc aagauuuauc ggggcaucaa agauccccug     2460

uguacgaagg agcucgugga cgaacugagc aaaaauguua gagccaaccu uauuaagaca     2520

aaggaguacc uguacuauca gcaacucggg cgggaugcgc ugaccggaga aaucauugac     2580

aaaacagauc ugcgcaauua cgccgugcuu cacaucauuc cuagaucugu cgugguugac     2640

gauagcauca acaaucuggu gcucaccaag gucaaaaacg cuaaggaguc caucguggug     2700

caguauggca gcaaaaauau uucugacauc uccaugagcg uuaaggauuu cuggaaccgg     2760

cugcagaagc ucgggcuguu uuccaaagga aaacuuagaa aucugaccac agaccucacg     2820

gaacugaacc aguacgagaa acaaggcuuc auugcccgcc agcuggucga gaccaaucag     2880

aucgugaaac uucuggcuac aauucuccag gggcaauuuc cccauaccaa aaucaucgaa     2940

gugcggcagg agcagauugc caacaucaga uaucaacugg auauuuaccg ccucagagac     3000

cugaacgucu auuaccgggg cauggacgcg uaccuugccg cugugguugg aacauaucug     3060

uacaccgugu auccgaaggc ccgcagauuc uuugucuacg gcaaguaucu gaaacaggau     3120

aagaauaacc gggaagaggg gcaguucaaa acgucuggac acagcuuuaa uuuccucugg     3180

aagcuguuuu acggcgggga ggacgaaauc uaugugcagg gaacaaacaa uauugcuuuc     3240

aacaaaaagg aucuucugca gaaaaucaag gagguuaaua acuacaaguu ucaaaaugug     3300

ucccucgcca ccggcacccg cagaucugac cuguucaaac agacaaucua cccucggcag     3360

gaacgcgaug ucaagaaaag cagaacccuc auuacaaaga aaaacgggcu guuuacggac     3420

aucuauggcg gauacaccgg caaugugaac uccuauuucg uucuugugca aacaaaguac     3480

aagaaagauc ccgacaaguu caaucuguau ggggucccca accgguacgu ggccaugcug     3540

gagcagucua aaagcaaggg aaauuaugaa accaaacuca aggaggugau ggagccuauu     3600

aucaagaacu ccaauaaacg caugauuggc uacaagaucc ugaaagaucg gauucuuuac     3660

aaccagauca ucgaagacaa cggguauaag uuuuucauua caagcucuca guacagacac     3720

aauuuuagac aaaugauccu guccgaucag agcaaaaaga uucucaugga cuaugucuuc     3780

gacccgaacu uuuaccgcca uaagaaaacc gaggauguua auaacgacaa ggaugcuaaa     3840

cugaucaagg uguaugacga aauucucuac cagaucgauc acuaucugca gaucuucacg     3900

auuggacauc acaucgacaa acuuaagaaa gcgcggcccc uguuugagaa gugcacccug     3960

gccgaaaagg cucaagugcu caaugauauu cugaucauug ccggcucuaa ccagacagag     4020

cuugcccuga aaccucucaa gaccacaaaa cugaccuucu ccaagcagaa uuguccccuc     4080

acaaaagacg cuaucuuuua cuaccaaagc ccuaccggga ugaucacgag aaaggagucu     4140

auuaaggaua uccugaaaca gaacauuuga                                      4170


<210>  4397
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4397
augagcagaa agaacaucgu ggaggacuac gccauuuccc uggauaucgg caaugcuucu       60

gucggguggg uugccuucac ccccaacuau cggcucgugc gcgcgaaagg acacgaacug      120

auuggcgcua gacuuuuuga gccugccagc acagccgagg agcggcgcau ggcuagaacg      180

acccggcgca gauacucccg gcgcagaugg cggcugcgca ugcucgacuc uauguucgau      240

caggcccuga gcgaagugga cccguccuuu cuuaugagac ggaaguauuc uuggguccau      300

cccgaggaua aaaauaacga ggcucacugg uucgggggag ugcuguuuga cagcaaugaa      360

caagauaagc gcuucuacga gaaauauccu acaaucuacc aucucagaaa gucccugaug      420

gaagacgaug agcggcacga cauuagagag guuuaucugg ccauccauca caugaugaag      480

uaccgcggca acuuucucac cgaaggggag cuggcgccca gcucuguguu cgaugucgaa      540

gacuuucuua aacugcucac agagauccug caguacgccg augaaggaga gaccgacgag      600

gaccuugaac ugcaggcuga ugccaauggc cucacagagg cucugugcac cacgaagggc      660

ucccggagca uuagagccaa aaacgcgauc ucuauucuga aggaaggggc cgcucucucc      720

aaacgccagg aggccaucau uagcgcugug uucgccggac uggaaggcaa uaaacuugac      780

cugaccaagg uuuuuccuaa caaagaguau acacccgagg auaagaaaac ccucgcccug      840

uacuucaaua aggcugacua ugaugaaaaa cggguggcga ucggggacuc uggacuucug      900

gaggauaacg aaugugacuu ucucgauugc auuagacgcc aauacuccgc caucgcucug      960

aagcagcugc ucggcgacuu cacaagcauc uccgagucua ugugugcccg guaugaacag     1020

caccagagaa auugggaucu gauuaaacgc uacugccggg aggagagcaa cgccgaaaau     1080

aucaacaaga acuauggggc ucuugucgga ugggagauug ugggcggcca aagacgcucc     1140

aucagagggg ccaaggacua cgaaaauaaa agaaaggcgg cuaacaaauu cuuugaggag     1200

cugauuaagg gaagcgcccu cccugaccag gaaaaagagc ggcugcuuca ugauaucgaa     1260

aaugacgagc uguucccggu gcagcgcgau ucuggcaacg gggucauccc cuaccagcuc     1320

caccugaaug aacugaagaa gauuauccaa aaacagggaa gauauuaccc uuuucuccug     1380

gagaccuaua uuaaggacgg caaagagguu aacaaacuug aagggcugcu cgccuuccgg     1440

gugcccuacu augugggacc ucuggucucc agagaugaca ugcagagcuc ugauaaucgc     1500

gagaaccauu ggaugauccg gagaaagaag ggcgugauua cgcccuggaa uuuugacgaa     1560

augguugaua aggacaaaag cggccacgag uucaucaagu cccugguggg gacagauucu     1620

uaccuucucg gagaaaccac acugccgaaa aacagccugc ucuaucaaga guuugagguc     1680

cugaaugaac uuaacaaugu gcgccugucc aucagaaccg gcaaccacug ggcugacaag     1740

cggcgcguua gacucgggca uaaugagaaa aagcugcugc uugaagaacu cuucgccaag     1800

caggcuaccg ugacaaagaa ggccgcggaa aaccugcugc ggcgcacgua cggaaacacc     1860

uacgagauua gcggccucgc cgaugagaaa aaguuugucu cuucccugag cucuuauggg     1920

aaaaugugua gaacacuuaa ggcuuccuac gugaauagcc acaaagacgu cguggaaaag     1980

aucauugagc ugcagaccgu uuucgaagac aagaaaacac ucaagcauca gcugcaacug     2040

cuuggcgagc ucgccgagac caacugcgaa cugcugucca auacgcacua uacaggaugg     2100

ggcaaacucu cucggaagcu gcuuaccagc aaaguggucg uguguaagau cgggaacgau     2160

uuugcuccug ccaagcauuc cauuaucgag auuaugcgcg aaaccgacag aaauuucaug     2220

gagaucauca cagauaaaga ccugggaguu ggcgaaugga uuaaccagca gaaugugggg     2280

gcggaggaug gaaacucuua ccaggacauc auugaugacc uccgggucag ccccaaggug     2340

aaacgcggca ucaaucaagc cauuagagug aucgaugaca ucuccaaggc uguugggaaa     2400

gagccuagcc ggauuuuucu ggaacuggcc ggcgaugucc agcagucugc uagaaccgug     2460

ucccgcaaga gccggcuuca gggacucuau aagucugccg gccugcaaaa agaguucaga     2520

gaccuggccg aaucccucaa cgagugcagc gauaaugacc ugcgcgacga ucggcuuuuc     2580

cuguacuaua cacagcucgg gaaggacaug uacacgggag aaccccugaa ccuggauaga     2640

cuuucuagcg acuacgauau cgaccacauu aucccccagg cugugaccca gaaugauucc     2700

cuggacaacc gcguccucgu ggcgcggguu gccaaugcua gaaaaacaga uagcuuucaa     2760

uaucugccug agauugccaa ccgcaugcgg aacuucuggc aggagcuccu ggacgccggc     2820

cuuaucucua gagugaaguu ugaucggcug aaucgccaga acgacuucuc cagccacgaa     2880

aaagagagau uuguccaacg gucccucgug gaaacccgcc agaucaugaa gaauguugcu     2940

acaauuauga gaaagcggua cgggaacucu gccgcgguga ucggacugaa uagcgagcug     3000

accaaagaaa ugagacagua ucuuggcuuc ucccauaaga accgcgacau uaaugauuac     3060

caccaugcuc aggacgcccu gugucucggg aucgccggcc aauuugcugu caaccgggga     3120

uucuuugcca auggcauggu gucugauggg gcgguuaaca gcuauaaucu guaccuccag     3180

gacuaucuga gaggauaccg cgagaaaaug uccgcuggcg agcggagacg caacaaggcc     3240

uucggguuua uugugggauc uaugaauagc caggauuccg acaaacggau caacccgaag     3300

acgggcgaaa ucgccuggga ugacgaggau aaagucuacc uuagaaaggu gaugaauuau     3360

cgcaagaugc ugauuaccca gaaagugggg gacagcuucg gcgcucucua caacgaaaca     3420

agauauggaa ccucugccaa ggagggcguc aacgggaucc ccuuugauaa acacaagcaa     3480

gacacagcuc uguacggagg cuucuccagc gccaaaaccg uuuauguggu gcugauugag     3540

gcgaagaaga aaacgcggcu ugucaauauc acaaugcagg aauacgccga gcugggggau     3600

cacccuucug acgacgcucu ccgcgccgug cuggcuaaga aaaagcccga auauggaaaa     3660

gcccggauuc uccugcggca uaucccuccc augcagcuua uucgcuacgg cggcgcccug     3720

guuaccguga aguccgcuac agagcugaac aaugcgcagc aacucuggcu gccguacaag     3780

gaauauugcc uuuucgauga cgucaucagc ucugccaucg auguggacga ggcuagacug     3840

ugucagaugc ucgauguuau ucuggacgcc auccagaaau acuauccuaa gcaacgguuu     3900

gaugagaccg ggcucuccgc cuuccacaac gcuuuuaaug aagugagcgc cgaggacaaa     3960

aagagaauug ucggagaaau ggugggcaug cugcaugcgg augcuaaaaa cgccaaucuu     4020

aaggcccugg ggauguccgg acgcuggggc cggaugagca accagucugg guacaccuuc     4080

gcugacagcg augaguuuau cuuccagucc cccucuggac uguuugaaaa gagaauuagc     4140

guuggcgagc ucaaaaagcg ccgggccaga uga                                  4173


<210>  4398
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4398
augaaggagg ugaaagacua caucauuggc cuggauaucg ggaccaacag cugcggauau       60

gucguuacag acaagcagaa uaacauucuc aaacugaagg gcaaaacggc caucggggcu      120

agacuuuuca aggaaggaga ggccgcggcu gaucggcgcg ccuuuagaac cacacggcgc      180

agacuggccc ggcgcagaug gcggcucggc cugcuugaag agauuuucga cgaagagaug      240

gcuaaggugg auuccucuuu cuuccgcaga cugaaagaga gcgacuacuc ccccaaggau      300

ucucggaaac aauuuaauag caucguguuc gaauccucug aggccgacaa ggauuuuuau      360

gacaaauacc cuaccaucua ucaccuccgc aacgcucuga ugcaugauga ccagaagcac      420

gaucugagag aaauuuuccu cgccguccau cacaucguga aguaccgggg gaauuuucug      480

cgcgaggaca gcguuaaagc guucaaggcc gcuaaauuua cacuucaggg agaggacggc      540

auuggcccgg uggauaagcu gaacgaccuc cugaaagaaa ucuauauuga ggccgcuccc      600

gaacuugcca ccgauaaucu guccaagauc gaggaaauua ucaaagacaa gaagcucuac      660

aaacaggaua agcugaaaca agucgcgaca cugcucacca aggaggccga cacgaaagau      720

aaggcuaagc ugaacaaaga cguggccaag cagguugcua augccuucau gggguacaug      780

aucauuuuug auaccaucuu caaccuuagc gacguggauu cuaaagacua uaaggucaaa      840

uucuccgacg ccaauauuga ugagaagcug gacacacuca ccagccugcu uacagauaag      900

cagaccgaau uugugcugga gcuccagaac aucuacaaua cgauugugcu gaacgaaauc      960

gucccugacg gaaaaucucu guccgagagc augguuaaga aauaugauga ccauaaggaa     1020

gaucucaaac uguucaaggc uuaccuuaau ucucugacag acgugaagaa agugaaggag     1080

cucaaagcgg ccuauguccu guacgugaac aacagaaagg cuaaucuucu ggccgccaaa     1140

aagcuccugg gcaaaaaggc ugauaacaug aauaacuuug agaucuucuc caaguuuguu     1200

gccgacaauc uggaugacag cgaacucgcg aacaaaauuc gggcuagacu ggaucuuggg     1260

gaguuccugc ccaagcaacg caccaaucag aacggaguga ucccuuauca gcuccaccag     1320

gucgaacuga cacaaauucu ggagaaacag ggcaaguacu accccuuucu uaucgccccu     1380

aauccggugg aaucccaccg gaacaaugcc cccuauagaa uuucugagcu cguuagcuuc     1440

cgcgugccuu acuaugucgg gccccugauc gacccuaaca augcugaguc cgaacaggac     1500

aaagccaaca gcucuuuugc uuggauggug cggcaaaagc agggacagau cacccccugg     1560

aauuucgagg aaauggucga uaccacagag uccgccaaca aguuuauuaa aagaaugacg     1620

accaaggaca cauaccugcu cggcgaggau gugcugccgg cgagcucucu uaucuaucag     1680

aaauucguua uucugaacga acucaauaac aucaagguga auggcauuaa acugaccagc     1740

gaggaaaagc aucaaaucuu ugacgagcug uucaaggucc agaaauccgu gucucuuaag     1800

aaccucguua auuuccugac agcccacggg uguguggcuc cuaacgucac cggacugagc     1860

gauggcacga aauuuaauuc cagcuacucu acauauauug acuaccgcaa gaucuucggg     1920

gccgaaaucg auaacaaaga caagcaggcu gauuuugaga agaugauuga gugguccacc     1980

guguucgaag accggaaaau cuuuaagaga aaacucgagg aaauuaccug gcugacaccc     2040

gaucagauca agcaagugag cucuaaacgc uacuccggau ggggccggcu uagcaagaaa     2100

cugcucaccc agauuacaga cgagaauggg guuaacaucc ugcagagacu guggaaugaa     2160

uccgagaacc uuaugcagau ccucucugau ccugucauua aggagaagau cguggccgcg     2220

aauagcuccu ucacgaaagc caccgacaug gaagacauuc uggcugaggc cuauacaucu     2280

ccccaaaaca agaaagcuau ccgccaggug augcgggucg uggaugacau uaucgccgcc     2340

gcucauggaa agaaacccag ccagaucgcg auugaauuug ccagagagga ucaggacaau     2400

ccucaaauga ccaagucccg gaaggcucag cuggaucaga ucuacgccaa aauuagcgac     2460

gaauuucucg auaacgccgu uaagaacgag cugaaaaaua ugaaggacaa caaaccgcuu     2520

gcuaaggaua agguguuucu guauuucaaa caaaugggcc gcgacgccua cacaggcgag     2580

aagcucucuc uggauaaucu gcaggacuau gacaucgauc acauuuaccc ccaguccuuu     2640

aucaaagacg auagcuucga caacagaguc cuugugcagc gggcgguuaa uaacgggaag     2700

ucugauaaug ugccuguccu ccuguuugga aacaaacaag cugccgaccu gggcaugacc     2760

aucagacaga uguggaagaa guggcucgaa cuggggcuua uuuccaaacg caaguucaau     2820

aaccugauca cggaucccga gaaaauuagc ucuuauaugg ccagcggauu cgugaaucgg     2880

cagcucguug aaaccuccca ggugaucaag cuggucgcua acauucugca agccaaacuu     2940

ccugacacag agaucauuga ggugaaggcg agcuacaauu cuauccugag aaaggaauuu     3000

aaccucuaca aaucccgcga ggugaaugau uaucaccaug cuaucgacgc cuaccugacc     3060

acaauugucg gcaacuaucu cuaccagguu uaucccaagc ugagaccgua cuucguguau     3120

gggcaguuua aaaaguucgg caaagccgaa agcaaggagg augacccugu gaaaaagauc     3180

aagcaguuua acuucauuua ccaacuucug uggggaaaag augacgcuau cugcauuucc     3240

cacaccgacc agcaggucuu uucucggagc gauaucauca auaagcucac gacagccuac     3300

aacuucaaau auaugaaugu gucccaagaa guuuacacca acucuaauaa cguguuuaag     3360

cagacagucu uccccauuau cgagcgcgac accgcuaaaa gcagaucccu gauuccuaag     3420

aagcuggauc ggcccaccga caucuauggc ggguacucua auaacaauga ugccuaucuu     3480

gcgcugguga aaguugacaa gaaaaaggga agcgaguacc gcguggucgg cauuccuaug     3540

agagccgcug ccaugcucaa ggcuuccaaa aacuaugaug aagaccugcg gaaggugauc     3600

gagcccaugg uuaugcucga uaaaaauggg aaggccaaac gcggaaucga aggcuuuaga     3660

gugcuggucg ggaagaugcc guucaaacag gccgugcuug acggcgauaa gaaguucaug     3720

cugacaggaa cgaacgacau gccuaaucuc aaacagcugg ugcugagcca acagaacaug     3780

aaguacauuc uugauuacau cgacgacauu gauugucgga aacauaaguu uggcaauaaa     3840

acccaggacg ggaacaagug ccugcucgag gucuucgaug aaaucauuca gaucguugac     3900

aaacacuuug agcuguucga uaaucgcucu uuuagagaga agcuccggaa cuccagagug     3960

aacuucauua aucuggcucu uagcaagaaa gcggaagccc ugcuggcuau ccucaacgga     4020

cugcaugcca auucuaugcg cacaaccauc aaggagcuua aaauuuccga ccugggccgg     4080

auccaaagcu cugugauuuc ccccgaagcc cagcucaucu aucagagccc cacagggcug     4140

uuugagagac gcgucaaggu gaaagaucuc uga                                  4173


<210>  4399
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4399
auggagaaca agcaguacua uaucggccug gacgugggga ccaauagcgu cggaugggcc       60

guuacagaua cguccuacaa ccuccugaga gcuaaaggca aggacaugug gggagcccgg      120

cuuuucgaaa aagcgaauac cgcugccgag cgcagaacaa agcggaccuc ucgcagacgg      180

agcgaacgcg agaaagccag aaaggcuaug cugaaggaac ucuuugccga ugagauuaac      240

cggguggacc ccuccuucuu uauccgccug gaggaaucua aauucuuucu ugaugacaga      300

agcgagaaua accggcaacg cuauacacug uucaaugaug cuaccuuuac agacaaggau      360

uacuaugaaa aauacaagac cauuuuccac cucagauccg cccugaucaa cucugacgag      420

aaauuugaug ugcggcuggu cuuccucgcg auucugaauc uuuuuagcca ucgcgggcac      480

uuccugaacg ccucccucaa gggcgacggg gauauccagg gaauggacgu guuuuauaau      540

gaccugguug agagcugcga auacuucgag aucgaacuuc cuagaauuac gaacaucgau      600

aauuucgaga agauucuguc ucagaaaggc aagucccgga ccaaaauccu cgaagaacug      660

agcgaggaac ugucuauuuc caagaaagac aagagcaaau acaaccucau caagcugauu      720

ucuggccuug aggcuuccgu ggucgaacug uauaauaucg aggauaucca ggacgaaaac      780

aagaaaauua agaucggguu uagagagagc gauuacgagg aauccucucu caaagugaag      840

gagauuaucg gagacgaaua uuucgaucug guugagcgcg ccaaaagcgu gcaugacaug      900

ggccuucugu ccaacauuau cgggaauagc aaguaccucu gugaagcucg ggucgaggcc      960

uaugagaacc accauaagga ucugcugaaa aucaaggaac uccugaaaaa guacgacaaa     1020

aaggcguaua augauauguu uagaaagaug acagacaaaa acuacucugc cuacguggga     1080

uccgugaaua gcaacauugc uaaggagcgc cggucugucg acaaaagaaa gaucgaagau     1140

cuuuauaaau acauugagga caccgcccug aagaauaucc cggaugacaa caaggauaaa     1200

auugagaucc ucgaaaagau caaacugggc gaguuccuua agaaacaacu gacagcuagc     1260

aaugggguua uucccaacca gcuccagucc cgcgaacugc gggccauccu gaagaaagcc     1320

gagaauuauc ucccuuuucu gaaggaaaag ggagagaaaa accuuaccgu gucugagaug     1380

auuauccagc uguucgaauu ucaaauuccc uacuaugugg gcccucucga caagaauccc     1440

aaaaaggaua acaaagcuaa uagcugggcg aagaucaagc agggcgggcg cauucugccu     1500

uggaacuucg aggacaaagu cgaugugaag ggauccagaa aagaauuuau cgagaagaug     1560

guucggaaau gcacguacau cagcgacgaa cacacacugc cgaagcaguc ucuucucuau     1620

gagaaguuca uggugcugaa cgagauuaau aacaucaaaa uugauggcga aaagaucucc     1680

gucgaggcca aacagaagau uuacaaugac cuguuuguga aagggaagaa gguuagccaa     1740

aaagauauca agaaagaacu caucucucug aacauuaugg acaaggacuc cgugcuuagc     1800

ggaaccgaua cagucuguaa ugcuuaccug uccucuaucg gcaaauucac cgggguguuu     1860

aaggaggaaa uuaacaaaca gagcaucguc gacaugauug aggauaucau cuuccucaag     1920

accguguaug gagacgagaa gagauucguu aaagaagaga uuguggaaaa guacggcgau     1980

gagaucgaca aagauaagau uaaacgcauc cugggcuuua aguucuccaa uugggggaac     2040

cugucuaaga gcuuucuuga gcucgaagga gccgacgucg gcacagggga ggugcggucc     2100

auuauccaga gccuguggga aacgaauuuc aaccugaugg agcuccuguc uuccagauuu     2160

accuauaugg augaacuuga gaaacggguu aagaaacugg agaagccccu cagcgaaugg     2220

acaaucgagg accuggauga cauguaccug ucuuccccug ugaaacgcau gauuuggcaa     2280

agcaugaaga ucgucgauga aauucagacc gugaucggau augcccccaa gagaauuuuc     2340

guggagauga cacggucuga aggcgagaaa guuagaacca agagccgcaa agaccggcuu     2400

aaggagcucu acaaugggau caaagaagac uccaagcagu gggucaagga gcuggauagc     2460

aaagacgaau cuuauuuuag auccaagaaa auguaccugu acuaucucca gaagggccgc     2520

ugcauguaca gcggagaggu gauugaacug gauaaacuua uggacgauaa ccuguaugac     2580

aucgaucaca ucuacccuag auccuucgug aaggacgauu cucucgacaa ucugguccug     2640

gugaagaaag agauuaacaa ucggaagcaa aacgauccca ucacgccgca gauucaggcu     2700

agcugucagg gcuuuuggaa aauccuucau gaccaagggu ucauguccaa cgagaaguau     2760

ucucgccuca caagaaaaac ccaggaauuu agcgacgagg aaaagcuguc cuucauuaau     2820

cggcagaucg uugagaccgg acaggccaca aaaugcaugg cgcaaauccu gcagaagucu     2880

augggcgagg auguggacgu cguguuuagc aaggcucgcc ucguuuccga guucagacac     2940

aaauucgagc uguuuaagag ccggcuuauu aacgauuucc aucacgccaa ugacgccuac     3000

cugaacaucg uggucggaaa uucuuauuuu gugaaauuca cccgcaaccc ugcuaauuuu     3060

auuaaggaug cccggaaaaa ccccgacaau ccuguuuaca aguaccacau ggauagauuc     3120

uuugaacgcg acgugaaguc caaaagcgag gucgcuugga ucgggcaguc ugaaggcaac     3180

uccgggacaa uugugaucgu gaagaaaacg auggccaaga auagcccccu caucaccaaa     3240

aaggucgagg agggccaugg aucuauuaca aaggaaacca ucguuggcgu gaaagagauu     3300

aaguucggga gaaacaaagu ggaaaaggcg gauaaaacac ccaagaagcc uaaucugcag     3360

gccuaucggc cgaucaaaac cuccgacgag agacugugua acauucuucg cuacggaggc     3420

cggacgagca ucuccauuag cggguauugc cuggucgaau acgugaagaa aagaaagacc     3480

auccgcucuc ucgaggcuau ccccguuuau cugggacgga aagauagccu cuccgaggaa     3540

aagcugcuua auuacuuuag auauaaccug aacgacggcg ggaaagauuc ugugagcgac     3600

auucgccucu gucugccuuu caucuccaca aauucucugg ucaagauuga cggcuaccuu     3660

uacuaucugg gaggcaagaa cgaugaccgg auccaacucu acaaugccua ucagcugaaa     3720

augaagaaag aggaagugga guacauuaga aagaucgaaa aagcuguuag cauguccaag     3780

uuugaugaga ucgaccgcga gaagaacccc gugcucaccg aagagaaaaa uauugaacug     3840

uauaacaaga uccaggauaa auucgagaau acagucuucu cuaagcggau gagccuugug     3900

aaauacaaca agaaggaccu guccuuuggg gauuuccuca aaaauaagaa aagcaaguuu     3960

gaggaaauug accuggagaa acaaugcaag guucuguaua acaucauuuu caaucuuucu     4020

aaccugaagg aaguggaucu cuccgacauc ggaggcagca aaucuaccgg gaaguguaga     4080

ugcaaaaaga auaucacgaa cuacaaagag uuuaagcuga uucagcaguc caucacagga     4140

cucuacagcu gcgaaaaaga ucugaugacc auuuga                               4176


<210>  4400
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4400
augaaggaga ucaaagacua cauuaucggc cuggauauug ggaccaacag cugcggauau       60

guggucacag acaagcagaa uaacauccuc aaacugaagg gcaaaacggc cauuggggcu      120

agacuuuuca aggaaggaga ggccgcggcu gaucggcgcg ccuuuagaac cacacggcgc      180

agacuggccc ggcgcagaug gcggcucucc cugcuugaag agaucuucga cgaagagaug      240

aauaagguug aucccagcuu uuuccgcaga cugaaagagu cugacuacuc cccuaaggau      300

cggcgcaaac aauuuaacuc uaucguguuc gaaagcuccg aggcugacaa ggccuuuuau      360

gaaaaauacc cgaccauuua ucaccucaga aaugcucuga ugcaugauaa ccagaagcac      420

gaccugcggg agaucuuccu cgccgugcau cacauuguca aguaccgcgg caauuuucug      480

agagaggauu cugugaaagc guucaaggcc gcuaaauuua cacuucaggg ggaagacgga      540

aucggccccg uugauaagcu gaacgaccuc cugaaagaga uuuauaucga agccgcuccu      600

gagcuugcca ccgacaaucu gagcaagauu gaagauauca ucaaagacaa gaagcucuac      660

aaacaggaua agcugaaaca aauugcgaac cugcucccca aggccgugga cuccaaagau      720

aaggcuaagc ugaauaaaga caucgccaag caggucgcua acgcccuuau gggcuacaug      780

uuccggcugg auacacucuu uagcuucacc gacguggaug uuaaagacua uaagcugaaa      840

uucucugaug ccaauauuga cgagucccuu gacgcucuga cgagccuccu gaccgaugcg      900

cagaucgagu uugugcugga acuccagucu auuuacaaca caaucguccu gaaugagauc      960

gugccugacg ggaugucccu uagcgaaucu auggugaaga aguaugauga ccauaaaaag     1020

gaucugaaac ucuacaagga guauauugac ucccugagcg aucaaaagaa ggccaagcag     1080

cuugaagcug ccuacgcccu guaugucaac uacagaaaag cugaccuccu ggccgcgaag     1140

aaacuguucg agaagaaaaa gaucggagau aaaaaguuug uugacgugau uuccaacuuc     1200

gagguguuug gcaaguucgu cucugauaau cucgacgaua gcgaacuggc uaacaaaauc     1260

aaggcccgcc uugaccuggg ggaguuucuc cccaaacagc ggaccaauca gaacggagug     1320

auuccuuacc aacugcacca gguugaacug acacagaucc uugagaagca aggcaaauau     1380

uacccguucc ucaucacccc caauccugug gaaucccaca gaaacaaugc ccccuaugag     1440

auuagcgagc uggucucuuu ucgcgugccu uacuauguug ggccccugau cgacaaccag     1500

uccauuaagg auaagcagaa uaaaaacaag uucgcuugga uggugcggca gaaacaagga     1560

cagaucacgc cguggaauuu ugaagagaug gucgacacaa ccgaaagcgc caaccaguuc     1620

auuaagagaa ugacacgcaa agauaccuac cuccuggcug aggacgugcu uccuaagucu     1680

agccugaucu aucagaaguu uaugauucuc gaugagcuga aucggaucaa aaucgacggc     1740

aagaaacuga ccuccaaacu uaaacaugau auuuucgaaa aacucuucaa gaaacaaaag     1800

ucuaucaacc uggacaaucu gaaaaacuac cuccuggccg agggcaacau ucccgggcuu     1860

aucgaaggac ugagcgaugg cauuaauuuu aacaauuccu ucagcacaua caucgacuau     1920

agaaacaucu uuggggauga gauugacaau ccuaacaagc aggcggauuu cgaaaagaug     1980

aucgaguggu cuacggucuu ugaggaccgc aagauuuuca aacggaagcu caaggaaauc     2040

accuggcuga cacccgagca gauuaaacag guguccagca agagauacuc uggauggggc     2100

cgccugucca aaaagcuccu uacccaaauc acagacgaaa augggguuaa cauccugcag     2160

cggcugugga augagcccga aacccucacg gaggugcugg ccaacccugu cauuaaaaga     2220

aagaucagcg aggcuaauuc ccuuuuugug cagauuaaca agguugaaaa uauccuggau     2280

gacgccuaua caucuccgca gaacaaaaag gcuauucggc aagugauccg cgucguggau     2340

gacaucauug ccgcggccca cggaaagaag cccagccaga ucgcuauuga guucgccaga     2400

uccucucaga auaaagcuaa ggugccugau acccggaaga aacaacucga caagaucuac     2460

aacaaaauua gcuccgaaau ccuggauagc ucuauuaaga acgagcugaa aaaucuuaag     2520

uccaacaagu aucucagcaa agacaagcug uuucuguacu ucaaacagau gggcagagau     2580

gccuauaccg gcgacaagcu cucucuggau cagcuucaga auuacgacau cgaccauauc     2640

uuuccccgcu ccuucauuaa agaugacagc cuggauaacc ggguucucac acaaaagccu     2700

aucaaugcca agaaaucuga cuacgggauu cccgcucugg aauuuggaaa caaguauguc     2760

ccggaucugg gcaucaccgu gaaagagaug uggaaacuuu ggcaggagaa ugggcugauu     2820

agcaaaucca agcucaucaa ccuguguaca aauccuaaaa agaucggaag caagagagcg     2880

ucuggcuuca uuaaccgcca gcucguggaa acgucccagg ucaucaaacu gguggccauu     2940

auccuucaag cugaguuccc cgacaccgaa auuaucgagg uuaaggcccu gcagaauaca     3000

acccucagag agagcuuuca ccuguacaaa aaccgguccg ugaaugauua ucaccaugcc     3060

aucgacgcuu accugacaac cauugucggg aacuaucuuu accaggugua uccuaagcug     3120

cgccccuacu ucguuuacgg ccaguuuaaa aaguucaauc aagaaaagaa caucgauauu     3180

cucaaaagac ugaagaacuu uaauuuccuc cggcagcuga ucuuuaacac ggacgauaau     3240

auuuauaucu cuggaaccaa agagauugug uucaacaaga aagacaucau ccacaaacuu     3300

gaaacagccu acggcuauaa guacaugaau auuagccgcg agugcuauca ggaaaccucc     3360

ucucuguuua accaaacacu cuacgcgcau aauagcuccg ucaagaacuc ucugaucccu     3420

aagaaaaagg ggcugcccac cgagauuuau ggaggcuaca gcgggaauaa agauuccuuc     3480

uuugugcuug uuaagaucgu gaagaaaaga acgaaccugu accggauugu cggaaucccg     3540

acacgcgagc ucgcuaagcu gaauagcucu aacaauuaua accaggcccu caauaaaauc     3600

guggaaucca agcugugucu uaaagagacc gagagcuuca agauucugau caaaagacug     3660

cucuacggca cacugauugu ggacaacggg cagaaguucc ggaucggcuc uuuuaaggaa     3720

aaacacaaug uccagcaacu uguucugcag cucaagucca ugaaauauau uaaguucuac     3780

aucgacggag gccagaacua uuuuaccgau guggaacgca aaaagcugga gaagcaggac     3840

agagauaaau gccuccugua cguguucgac gauaucauga acgucgugaa uaagcgguuu     3900

acccuuuucg acaugagcaa auaugagaag gauggggacu cucugagaga aaaauuuaac     3960

ugucuggauu ucaaugacaa gguuuccauu cucagcgauc ugcuuaaggc cuuucaugcu     4020

aacuccgacc gcacaagcau cacgaaacug aagauuacca aucucggacg gcaccaagcc     4080

ggcaaaaacg ggaucacacu gaccacaaau gcgcagauua ucuaccaguc uccuaccgga     4140

cucuucgaga gacgcauuaa gaucaaagau cuguga                               4176


<210>  4401
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4401
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaac acgagcauga acaguccucu aucgucaagc ugggcguuag agugaacccc      120

cucagcgugg augagaaaac aaauuucgaa aaggggcggc cucuguccau uaacgccgac      180

cgcacgucua aaagaagcgc gcggcgcaau cuucaaagau uuaagcugcg gcgcaagaac      240

cucaucaauc ugcugcucaa acaccauauu aucuccaacg agaccauccu gacagagguc      300

ggaaagaaua ccacacacca gacccuuaaa cugagaucua aggcuacaca ggaaaagauu      360

agccucgagg aauuugcccg gauccugcuu uccauuaaca agaagcgcgg cuacaaaagc      420

ucuagaaagg agaaaaauga ggaugaaggg uucaccaucg acggaauggc cauugcuaag      480

gagcuguaug auaaccagcu cacgccgggc caauacgugu auaauauccu gcaggacggc      540

aaaaacuaca uucccgauuu cuaucggucc gaccugcagg ccgaauuuga ugacguuugg      600

caguuccaaa agaaauuuua cagcucuauc cucaccgaug aguucuaucu gaagcugaag      660

gggcagggaa aagaaaauac acgcaagcug uuucucgcua uccaucaggu guacaccgcc      720

gagaacaaag gcaagagaga cgcggucaaa cugcaguacu auaaguggcg guccgaggcc      780

gugaagaaac aacuuagcau ugaagagguu gcuuacgugc uggucgaaau caacaauaac      840

auuaauaagu cuuccgggua ucucggagcc aucagcgauc gcuccaaagc ucuguacuuc      900

aacaaugaga cagugggcga auaucuguac gggcagcucg ugaagaacag acacaauucu      960

cugcggaacc aggucuuuua uagacaagac uaccuugacg aguucgagaa aauuugggaa     1020

acccaggcca aguuuuacga ugagcugacg aaugaacuca agcaggagau ccgcgacguu     1080

gugaucuucu aucagcggaa acuuaagagc caaaaacacc uuauuuccga uugccuguuc     1140

gaaaagcauc acaaagcgau cccuaagagc ucuccccucu uucaggaguu caagauuugg     1200

cagaaccuga auuaccugga guuugugcau acacagucca aagaaaagau cuucuuuagc     1260

gagcucgacg augaaaccag acaauucguc uuugccgagc ugaacauucg cggaacacuu     1320

aaaaucaagg agaucaaaaa gcugauuggc cucgaaaccc cuguguauug gaaaucuaau     1380

uucgacgaga ucgaaggcaa ccggaccaau cagguucugu acaacgugua ucagaacauu     1440

gcugaugacc ugggguacgg auuugauugg agcaagaagc acgccuucga gaucaaugcu     1500

gaacuucagg ccauuuuuaa agacgucggc aucgauacag ccauucucaa cuucgacgcu     1560

uccaucgagg agaaugacuu ugauaagcaa aaaucuuauc agcuguggca ucugcucuac     1620

agcgcggaag acacggauaa ggccaccgag gaagacaaac ugaucuacgg gaacauuaau     1680

acaaagcuga agaaacagcu gcaccagaag uauggauucg aggcuaaaua cggcaaccuc     1740

cuggccaaua ucucccugca acaggauuau gggaaccuua gcgccaaggc uauuaaaaag     1800

auccuccccu accuggaauc uggaaauucc uucuaugagg ccuguaccau ugugggcuac     1860

aacccuagca auucucugac aaaggagcag caacagaaca gaaaacucaa ggacaaacug     1920

gaacuucugc cgaaaaauuc ccuccgcaac cccguugugg agaaaauccu gaaucagaag     1980

gucaaccuga ucaaccagcu uauugaugaa uauggcaagc cugacgaggu gcggaucgaa     2040

cucgcgagag agcugaaaaa gagcgcuaaa caacgcgccg aucuguccaa gcacauuaau     2100

gacgccaccc ucaaaaacca gaauauccgg aagacgcugc agaaagauuu ugggauucag     2160

aaccccucua agaaugacgu caucagauac aaacuuuacg augagcugaa agacaacgga     2220

uauaagacac ucuucaccaa uaaauacauc ccuaaggaac aacuguuuag caaagacgug     2280

gauauugagc auaucauucc caaggcuauc cuguucgacg auuccuuuuc uaacaagacc     2340

cuugccuauc gcaauguuaa ccucgcuaaa gccgacgcga cagccuucga uuuuauuagc     2400

uccaagcacc uggacgaucu ggacaauuac aaagcucggg uggaaacccu cuuccagagc     2460

aagagaaucu cuaaaggcaa guauaagaaa cugcuuauga cacggcagaa ccugccggag     2520

gauuuuaucg agcgcgaccu cagaaauucc caguacauug ccaagaaagc uacggccaug     2580

cugaaggaag ucuuccggag cgugguuccu accucuggga gaaucacaga uaaacugcgc     2640

gaggacuggg aucuugugaa cgucaugaag gaacugaauc uccccaagua ucggacccug     2700

ggacucacag aguuuauuga acgcaaaaac ggcaacaaga ucgagcaaau uaucgacugg     2760

accaaacgca augaccauag acaccacgcg auggaugccc ugacgguggc uuucaccaca     2820

cauaaccaca uucaguaccu uaauaaccug aaugugaccc augggcucaa cggcguugac     2880

acagaucacg agaagucccg gcuguacgcc aucaaacagg cuaucaccaa ggucuauauu     2940

gugggaaaga cgaaaaagcg caaauuuauc ccucccaugg aaaauuucag aagccacgcc     3000

aagaaccauc uggagggcau ucuuaucucu uucaaaaaua agaacaaagu ggucacaaag     3060

aauaagaacc ggaccaaaac aaagaccaaa gggguguuua augaaaagau ucaacugacc     3120

cccagaggac agcuccacaa agagacaauc uacggccaug ccaagaaggu uaucgugacg     3180

aaagaaaagg ucagcuccaa auucacccac gagaagauuc uucaggugac aaaaccugcu     3240

uauagagagg cgcuccugaa gcggcuugcc agcuuuaaga acgacccgaa aaaggcuuuc     3300

ucugggaaaa augcccuguc caagaacccc cucuacuuug auaucaccag cggaaagaag     3360

guuccugaca cagugaagcu guccuauuuc gaaaauucuu acgucauucg caaagaggug     3420

aacagcgaua acuuuaagaa ugaaaaaucc cugcagaagg uucuugacgu gggcaucaga     3480

cggauucugg agaaacgccu cgccgauuau aacgggaaug cuaaggaagc cuucucuaac     3540

cuggagaaaa aucccaucug gaaggacaag gagaaaggcg ucugcaucaa gaaagugaga     3600

auugaagcgc ucaagaacgc ugagccucug caugugaaaa aggaucaccu uggaaagauc     3660

auucuggacg acgaaaauaa aagcaccccc gucgauuuua ucuccacggg caacaaucac     3720

cauguugcca uuuacgagga caaggauggg aaacugcacg acaagguggu gucuuucuac     3780

gaggucguga gccggguuaa ccucggacug ccgaucauug auaaauccuu uaauagccau     3840

cuugacugga aguuccuguu cucuaugaag caaaacgaaa uguuuguguu cccuuccgag     3900

gauuuuaauc ccaaagaaau cgacaucggc aacccuaaga auaacgagcu cauuagcaaa     3960

uaucuguuca gaguccagaa guuuucuaaa gugacauacg ggaacuccgc cguucgcgau     4020

uauguguucc ggcaccaucu cgaaaccgag cugaucgaca agaaggagcu uaaaggaauu     4080

accuacuauc agcucaagag caucggcaug cuggaaaaac uggucaaggu gagaauuaau     4140

cacaucggga aaaucguuca cauuggcgag uacuga                               4176


<210>  4402
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4402
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc ccuugugaaa       60

gaggcugaaa augagaacga agucuccgau aucauuaagc ugggcguuag agugaauccc      120

cucacggugg acgagcugac caacuucgaa gggggaaaag ccuacucuaa uaacgcggag      180

cggacacuua agcgcggcgc uagacggaau cugcagcgcu auaaacucag acgggagaac      240

cugaucgaaa uucugcucaa gaaugggcug aucaacaagg auaccgcccu uacagagguc      300

ggaaaagaaa ccacacacca aacccugaag cuccgcagca aauccgccaa ugagaaggug      360

ucucuggagg accuugcuaa aauccugcuc agcauuaaca agaagagagg cuacaaaucc      420

ucucggaagg uuaaugccga agaugacggc guggcuaucg augggaugga ggucgccaaa      480

cugcuguaug acgaaaacau gacgccugga gaguacgugc uccgccugcu ugaaagcggc      540

aagaaacauc ugccggauuu uuauagaucc gaccuccagg aggaguucaa ucugguuugg      600

aagaaacaga gcgaauuuua cccccuuauu cugaccaagg aacucuucuu ugaacugcac      660

gggaagaaca aaucucagac augggcgauc ugcaaggagc cucugggaau ugugggcguc      720

aaacucaagg gguccgccaa agaacaaaag uucgcucggu aucgccugag aaaggaggcc      780

cuuacccagc agaucgagcu ggaacaucuc gcuauugugc ugcaggauau caacaaugac      840

agcaacaaau cuuccggaua ccuuggcgcc aucagcgauc ggucuaagga gcuguuuuuc      900

aauaaagaaa cagugggcca auaccaguau aagcagcucc agaaagaccc caacaccucc      960

cugaagaauc aagucuuuua ccgccaggac uaucuggaug aguucgaagu uauuuggaga     1020

aaccaggcga aguuuuaccc ucaacucacg aaugagcuga aacaggagau ccgggacgug     1080

gugauuuucu aucagagaaa acuuaaaagc cagaagaacc ugaucuccuu uugucaauuc     1140

gaaucuacac agcagaauua cgucgagaaa gaaaccggga agacaaagau ucgcaccauc     1200

ggacggagag ugaucccccg cagcuccccu cucuuccagg aguuuaaaau uuggcaaagc     1260

cugaacaauc ugaaguucgu uaacucugag uccaaucaga ucauucaguu uaucgaacuu     1320

gaggaugacg ugaaaaaggu cguguucgau gaacucaacc ugcggggcga ccugaaaccg     1380

aaucagguuc ucaagauucu gagcaaauau augaagaucg ggaaacuuac ccaauggaag     1440

ugcaaccugg aggaaaucga gggaaacaga acaaauucug cccucuacaa cguguuucag     1500

augauugcug aggaagaggg cuacggguuc aauuggggaa agaaaagcgc ccuggaaauc     1560

aaguccgagc ugaaagcuau uuucucuagc aucggcauua acgauuccau ccuugacuuc     1620

gauuuuagca agaagcucga ccagcagcug ucuuaucaac uguggcaccu ccuguacucc     1680

gccgaagagg gcaauagcaa aauuacggag gaagauaagu ucaccuaugg gaacacagga     1740

gucgcccuua aaaagaaacu gcaucucaag uuuggcuuca agcccgagua cucuacccug     1800

aucuccaauc ugcgcuuuac aagcgacuau gggaaccuuu ccucuaaagc uaucagcaag     1860

auucucccuu uccugcggga cggauccgau uacucuaaag cgugucagua ugccggcuac     1920

aaucacagca acucccugag cgcugacgaa cucgauaaua gagagcugaa gcccaaacuu     1980

gaacugcuca agaagaacuc ucugcgcaau ccuguggucg agaaaauccu gaaccagaug     2040

gugaauguug ugaaccaagu cauugacaac uacgggaagc ccgaugaggu gcggaucgaa     2100

cuugccagag agcucaaaaa guccgccaaa gaacgcagcc agaugaccaa gaauauuucu     2160

gacgcuacgc ggagaaacgc cgagaucaaa aaguaucugu ccaaggaguu cggcaucccg     2220

aauccuacaa aaggagaugu uauucgguac cgccuguggg aggagcucgc gagcaacggc     2280

uauaaggacc uguuuagaga uaaauacauc cccaaggaaa aacuuuucuc uaaugagauu     2340

gacaucgaac acauuauccc uaaggcucug cucuuugaug acagcuucuc caacaagacc     2400

cuggccuuua aaguggauaa ucugaagaaa gccgaccgga ccgcuuucga cuuuaugagc     2460

acagauuucu cuaaggacgu cgauuccuau aucgggagaa uugagacccu ucauaaagac     2520

ggaaagauca gcuccucuaa guacaaaaag cugaugauuc ccaaaagcaa gcucguggau     2580

ggcuuuaucg agcgcgaccu gcggaacaca caguauauug ccaaacaggc uagaaagaug     2640

cuccuggaag uguucaagac gguugucccc accacaggga aaaucaccga uaaacuucgc     2700

gaggacuggg aucugaucaa ugugaugaag gaacucaacu uuccuaagua caaagcccug     2760

ggacugacaa agaagauuga gagauucgac gugggcuccg aaaagaucaa gucuauuaau     2820

gucaucgagg gguggagcaa acggaacgau caccgccauc acgcgaugga cgcccuugcu     2880

guggccuuua ccacgcacaa ucauauucaa uaccugaacu aucucaaugc uagaaaggau     2940

auccaccaug agaugcacgg caccauuaaa uccaucgaaa accagaucau uggaaaggac     3000

guuaugggca aacggcgcuu caucccgccc auuucugacc ugagaaugaa ggccaagcuc     3060

cagaucgaga gcauucugau cuccuucaaa aacaagaaua aaguggucac auggagcgug     3120

aacaagacca aacuuaaggg gaaggauaau uacaacaaac ggcgccagcu gacaccuaga     3180

ggacaacucc auaaggaaac cguuuauggc aaaaucaagc ugauugaccc caaaccucug     3240

aaacuuaaua acaaguuuac gcuggugcgg gcgucuauga ucgucgauaa ggugcaccgc     3300

gagauuguuc uccagcaccu guccaaguac gacaauaaca gcgaaguggc cuucgauucu     3360

aaaacaauca agauugaccc cauccucauc gaggggauug gacugaaaga ggucgugugc     3420

uaugaagaug uguuuaagau cagaaagaaa auuuccggcg acaauuucaa gaacgagaaa     3480

caucuugaaa aggucaucga ugagaaaguu aagcagauuc ugaaagaccg ggugaucaag     3540

uuucagggga auuucaagga ggcuuuuagc aaccuggaag agaauccgau uuggcucaac     3600

aaaucuaagg gcaucgccgu gaaauccguc gcuaucaagg gagugaauaa cgccgaaagc     3660

cugcacgagg gcaaagauca uuucggggac caaauucuug acaagaauaa gaaacagauc     3720

ccuguugauu uuguguccac cggaaacaac caccacgucg gcauuuacga agacaaggau     3780

gggaaucugc aggagagagu gguuagcuuc uuugaggccg ugacacgcgu caacgaagga     3840

cuccccauca uugacaaaac cuauaauucu gauaucggcu ggaaguucaa auucaccaug     3900

aagcaaaacg agauguuugu guucccuagc gacgauuuug accccaauga auuugaucug     3960

acagacggga agaacgcugc gaaaaucucc ccuaaucucu accggguuca gaagauugcc     4020

acgaaaaacu acauguuuag acaucaccug gagaccgugg ucacaaauga ucuugaguuc     4080

accuauaagu cuauccgcag cacaggcugg cugcggaacc uggugaaagu gagaauuaau     4140

caucucggag aaaucgucua cguuggcgag uauuga                               4176


<210>  4403
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4403
augaagaaaa uccugggccu cgaccugggg accacaagca uuggaugggc ccuugugaac       60

gagaaggaaa auaacgagga acuguccucu aucauuagac ucggcguccg gguuaauccc      120

cugacggugg augagaaaag caacuucgaa aaggggaaag acaucaccac aaaugcugau      180

cgcaccaagu gcagauccgc ccggcgcaac cuucagagau uuaagcugcg gcgcgaggcg      240

cucgcugacu gucugaaaag acacggacug augacagaug agaccccucu cugcgaaaca      300

aauaacucua uuaucuucaa gaccuacagc aaucgggcca aagccgugug ugagaagauc      360

gcucuggacg auuuuguccg cgugcuuuuc augauuaaca aaaagagagg cuauaagucc      420

ucucggaaac ugaauacgga cgaugaaggg acccucguug acggaaugga uguggccaag      480

cugcuuuaca acgagcaaau cacaccgggc gaguaucugc uccgccuguu uaaaaagggc      540

aaaaagacca gacccgacuu cuaccugagc gaucucaaag cugaacugga gcggauuugg      600

gacuuucagc gcacauucua uccugaaauc cuuugcaaug cccugaagga ggaccucaag      660

gggaaaucca gaacccagag cucugcgauc uuuuacgcca aguauaaagu cuccacggcu      720

aagaucgccg gaaaagauaa gcggcugcag gaacuuacau ggagaaccga cgcucugcgc      780

aagcaacuca caauugauga gguggccuac guuaucugua gcaucaacgg cgaggugaau      840

gggucuuccc agcggcugag caugauuucu gacagaucca aacugcucua cuucaaccau      900

cagaccaucg gacaguaucu gaugcaacgc cuugaagaga accccaaugc gggccugaag      960

aacauuaucu uuuaccggca ggauuaugaa gacgaguuca augauauuug gaacagccag     1020

gccaccuacc acaaagaacu cacaccugag cugaagaaag aggucaagga caucaucuuu     1080

uaucagagag ggcuuaaguc cugcaaaugg cugauuaauu acugugaacu cgagcaugaa     1140

gagaucgaag ugaagacgcc cgagggaaaa cugcgcaaga uuaccaucgg cucuaaaaca     1200

ugcccuaaga gcuccccgcu guuccaagag uucaagauuu ggcaagugcu caacaaucug     1260

caggucagcc cccuuaacuc ucggagaaaa gcuaagcgca aaaaggauga ggaaccuucc     1320

cuguuuagcg acgaagauau gaauaccgcc ugucgguauc ucaacgagga ggaaaaacug     1380

acacuguuca aggagcuuuc uaucaaaaag gaacucaaga aaaaggacgc ucugaaacug     1440

cucuuugaga auuacaagga acuggaccuu aacuacaaag uuaucagcgg caaugugacc     1500

auggccaacc uguauagagc cuuccaggau auuaucuccc ucacggggaa uggagaguuu     1560

gacuuccagg cuaugcccgc ggaggaagcc gagaagacag uggaagagau uuuuggcggg     1620

cugggauaca agaccgcuuu ccugcacuuu gaugccucuc uucaugacaa agaguucgaa     1680

cgccagccug ccuaucggcu cuggcaucug cuguacagcu augcuggcga ucaguccaag     1740

accgggaucg aggcccucgu caaacggauu caagaacugu gcggauuuga gcccgaauac     1800

gcgcggaucc uugcuaacau uagcuucauc ccggacuaug gcaaccuguc uuccaaggcc     1860

augcgcaaaa uccucccuua caugcaggau ggcuacgagu auagcgaagc cuguucuaag     1920

gcuggguaca gacacuccgc cagcucccug acaaaggagg agauuaauaa aaagaacuau     1980

aaaacccacc uggacgaaau caagagaaau ucucuuagaa accccauugu ggagaaaauc     2040

cucaaucaga ugguuaacgu ggucaauagc gugauugaua cauacuccaa caucgacgaa     2100

auccgcauug agauggcucg ggagcugaag aagucuaaac cugaaagaga ggauaugagc     2160

uuugccaucu ccaaggcgac gaaagaaacc gagcgcauua agggaauccu ggaacaggag     2220

uucaaaauuc aacacguuac aagaaaugac aucauccggu aucgccucua cgaggaacug     2280

aagcccaacg gcuauaaaac ccuuuacagc aauacauaca uucccagaga ggaucuguuc     2340

ucuaagaagu uugacaucga acauauuauc ccuaaagccc gccucuucga cgauuccuau     2400

agcaacaaga cccuggagcu gcgggacauu aauaucgaaa aagggaacca gacggcuuac     2460

gauuuugugc uuucuaagua uggagaggag ggccucuaca aauaugaaca gagagucgag     2520

gaguucaaga agaauaacga acaggccggg accaaaugcc ggaagcugcu gaugcgcgaa     2580

gcugacaucc cguccgauuu ucucaacaga gaccugaaug auagccaaua cauugccaaa     2640

aaggcgcacg agcuucugcg ggagaucgug cccacaguca ccgccacaac cggcucuauu     2700

acgaaccgcc ucagagaaga cuggaaacug gugaauguua ugaaggagcu gaacugggaa     2760

aaguaugaua aacuuggacu gacagaggag uacaaggaca aagauggcaa uaccgugcgg     2820

cacaucaagg acuggacaaa aagaaacgau caucgccacc augcuaugga cgcccucacc     2880

auugcuuuca ccaagccuag cuuuauccag uaccugaaua accucaaugc ccgguccaac     2940

agagggggaa gcauuuaugg caucgaacag aaggagcugg aacgcgagaa uaacaaacuu     3000

gucuucauuc ccccuauuga ccccaagauc uuucggaaac aggccaagga acaccugucu     3060

aauauucuca ucuccauuaa agcuaagaac aaggugguua cgaaaaaugu gaacaagaca     3120

aaaagcaagu ccgggaccaa cagaaaaguc caacugacac cgcgcggaca gcugcaucuu     3180

gagaccgugu acggcaagau ccggacauau aaaaccgagg aaaucaaggu ggggacgaag     3240

cugacagagg aacucauugc gaaaguugcc gauaagaaaa ucagagaggc ucugaugcag     3300

cgccucucug aauacggcgg aaauauuaag aaagccuucc aaaacaucag caagcacccu     3360

auuuggcuga auggcuccca caccaagcau guccccgaga aagugaagau cguguuuuuc     3420

gaggacgucu uuaccauccg gaaagccauu acaccugaua ucaagauuga caaagugauc     3480

gauucuaaga uuagacggau ccuucaggaa cgccugaacg aguucggggg agaagcuagc     3540

aaggccuucu ccaaucucga cgagaacccc auuugguuua auagagauaa aggcaucgcg     3600

aucaagucug uuaaaauucg ggggaucgcu aagggaaccg ccauucgcgu gaaaaaggac     3660

auccacggca agacaauuau cgggaacaau ggcuauccug ucgccaacga uuacgugaau     3720

acgggaaaca aucaucacgu ugcuaucuuc agagacaaag auggcaaccu gcaggaacac     3780

auugugagcu uuuaugaggc caccgcgcgg gcuauccaga aucugcccau ugucgacaga     3840

aacuacaauu ccgagaagga cuggaaauuc cuuuuuagca ugaagcaaaa cgaauacuuc     3900

guguuuccga acccuaauac aggguucaac cccucugaga ucucccugaa agaugaaaag     3960

aauuaugccc ucauuagccc caaccuguuu cgcguucaga aacucucuca uuacuauuac     4020

guguuccggc accaucugga gaccaauguc gacacaaacc uucagcugaa ggauaucacc     4080

uggaagagaa ucacggccgu gaauaaccug accggagcug ugaaaguccg cauugaccac     4140

cucggccgga ucguugaugu gggggaguau aaguga                               4176


<210>  4404
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4404
augaagaacu accacaucgg ccuggacauu gggaccagcu ccgugggauu cgccgcuaga       60

gaugacgaua auaaccucau ccgggucaaa ggcaagaaug uuauuggggc ccgccuguuu      120

ucugagggac agacagcgca agaaagacgg auggcucgca cgagcagacg gcgcuauaac      180

agacggaaau ggcgccuuag acugcucgac gagaucuucc augaugaaau uauccacaag      240

gacgccgagu uuuucaaucg gcugaaacag uccuuucuuu cucccaagga ugaacgcaag      300

caguacuucg gcagccugcu cuuuccugag accggggacu ccgaguauua ccauaacgga      360

aaucagacaa ucuaucaccu gagacaucgg cugauuaccc aagaugaaaa agccgacauc      420

cgcgagauuu accucgcucu gagacacauc auuaaguauc ggggcaacuu ccuugaugac      480

acaccggugu cuagcuuuga agccuccgau cugaaacucg cuagcuucgu gcccaccauc      540

aaugaccugu uugagacaau ugacgucaac uacgaguucu cuauggauaa uaucaacgaa      600

aucgaggaca uucuucugaa uaacaagcuc agaaauuccg auaaaagcaa gcagcugacc      660

gaccugcuca cggaaccuau cgugaacauu gaugacaagu cucuggagaa acaguuuaac      720

aaggaacgca ccgauaucuc caaagagauu gccaagagcg uugugggcuc uaaaaugaau      780

gucaacaaac uucuguccuu cgagagcucc gaagggucua aaaucgcguu cagcuuuucc      840

gacgccagcu cugaggauaa gaugauggaa cucuccgaca uccuggauga cagccggaug      900

aauauucuua acauccugaa ggugauuuac ucuagaguuc gccucaauca gaucauuccc      960

agcggaaugg gccuguccga gucuauggug gcugccuaug acagccacaa acaacagcug     1020

aaggaucuca aaacacugcu uaacguccug ccuuucaaug aacgguacgc ugugaagauc     1080

gccuaugcgg ccuacaucgg gaacauugac aaacagaagu uuaccaaguc cgagaucaaa     1140

gcuauugaua agcucaaaga aaagagcgcc aucaugaaug ugcuggacuu caaaucugga     1200

cauauuacaa acgaggagcu uuccaauguc aucaaggcug uuauugugga uaaguaucug     1260

aacgacgaag auuuugagua ccacggcauc guggaaaaga ucaaucagga cauuagcaac     1320

gggucuuauc uccugaagca aagaaauaac cagaacggaa auauccccca ucagcugcac     1380

caaauggagc ucgaucagau uaucgagaac caguccaaau acuaccacuu ccugggcgaa     1440

cuuaauccua acagcaaucg caaccauauu gccaaguaua aaaucuccga gcuggucgcc     1500

uuucgggugc cguacuaugu uggcccccuc aucaccccug aggagcagga gaagacgucu     1560

ggggcuagcu ucgcguggau gaagcgcaaa aaggacggag ccauuacacc cuggaauuuu     1620

gaagagaaag uggauaagga agagaccgcu uccagauuca ucaaacggcu gacagucaag     1680

gacaccuacc ugcuuaacga agaugugcuc ccugacaauu cucugcugua ugagaaauuu     1740

aagguucuca acgagcugaa uguggucaag gccaacaaua aaagacuuac cguggaagag     1800

aagcaaggcg ccuucgacaa ccuguuuaaa aagaugaaaa cagucacggc uaagcgccuc     1860

aagaauuacc uggugagcaa ccacaaccau auuaauuccg uugaaaucac cgggcugagc     1920

gauggagaca aauucaacaa uucucuuucc acauauauug auuucaagaa aaucuuuggc     1980

gacaagaucg auaaccccgg gcuccuggac gauuucgagg acauuaucga auggagcacc     2040

guguuugagg aucggaaaau uuucgaggaa aaggucagaa aggacaucuc uuggauuucc     2100

gagagcgaaa ucaaaucugu gguuagcaag cgguacuccg gauggggccg ccugagcaaa     2160

aagcuccuga auugcaucac aaacaaugag ggcagaaaca uuaucgauga acuuugggag     2220

gagucugaaa ccuuuaugga ggccgugucc aaaccggaag ucaaggagca gauugacggg     2280

aucaauagcg auuucauuaa gaacacggac aucgaguccg ugcuggcgga cgcuuacaca     2340

ucuccucaga auaaaaaggc cauucgggaa acccacaaac ucgugaagga uauccagaaa     2400

gccaugggag gccaagcucc cgccagcauc uccauugagu uuaccagaaa cccucaggac     2460

aaggggcagc ugacacgcuc ucggcauagc gaaguuucca gaaaauauaa ggagaucucu     2520

aaggaaauua gcaaaacccu gaaugaugag cuuaagucca aaaagaacag cauggacguc     2580

gauaaauacu aucucuacuu caugcagaug ggaaucgacc uguaugaugg gucuuccauu     2640

gacaucgaug accugaauaa cuaccaaauc gaucacauua ucccccagag cuuuuauaag     2700

gacgacucuu ucgauaaccg cgugcucaca cacuacucca auaaccagaa gaaaggcaau     2760

aacacgcccc ugaauggccu uaacauuaau gacaagacca gagcuaucug gaaccaucug     2820

cgggaucucg gacugauuac caaaaagaaa uacaauggcc ugacccuuaa ggggauggac     2880

cagcucagca aguauacaaa acacggauuu auccgcagac aacuggugga gaccucucag     2940

gucaucaagc ugguggccaa cauucucucc gaugaguuca gcacggacga uaccaaaauc     3000

auugaaguua aggcgaaaau gaacucccag auccggcaag uguuuaaucu guacaagguc     3060

cgcgaacuua augacuauca ucacgccgug gaugcuuacc ugacaaccuu cauuggcggg     3120

uaucucuaca acagauaucc uaaucugcgg aacauguucg uuuacggaga cuuuaagaaa     3180

uucagcgccg auguggacaa ucugaagaca uuuaacuucc uucacgagau caccaacccg     3240

ucugauaaau uugaagaccg cauuuucgac gaaaagagcg gcgagcugau ccucgauaga     3300

aaacaugcua ucgaaacgau uaagaagauc uaccaguaua aauacaugcu gauuacacgg     3360

gaggucucca ccaagaaugg gcagcucucu gaccagacag uguauagcgc caaacgcauc     3420

aacaauguua aggcgcccau uccugugaaa aaggauuuua accccgaacu guacggcuuc     3480

agaaccggaa augucgacca ccauauggug aucgugaaau auccuaagaa ggauuccuac     3540

gacuauaaag ucguuggcau cccccuucgg uuugugcaaa agcugaaaga gaccaaggau     3600

aacaaagacu acgaaaaucu ccugaaccag auugugcaga ugaagcugaa uagaaagaaa     3660

caggauuuca ucgucguggu ugacaacgug auguacgggc aacuuauuca ggauggaggc     3720

cagaaguuua cacugggguc uagcacguau cgcuacaaug ccaaacagcu cgucaucgac     3780

aacggauauc ugaagcucau ugcugaugag cgguacaaaa auucccugac cgacgaggaa     3840

aagucuaacc aauauaucac acuuuacaau gagauccuga aggugguuaa cggcagauuc     3900

gacguguucg augucaacag cuuucgcaaa aagcucaaua acggggugga caaauucauu     3960

aagcugaaua ccguugauaa acggaucauu aucaugaaga uucuguccgg ccuucacugu     4020

ggaccgagca ucgugccucu gaaggaacuc ggccugacaa caccccucgg gcagcugcag     4080

ucuacguccg gaauuagccu uucugagaac acaaucauca uuuaucaauc cccuagcggc     4140

cuguuugaga gaaaaguccg ccugggggac cucuga                               4176


<210>  4405
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4405
augaagaacu accacaucgg ccuggacauu gggaccagcu ccgugggauu cgccgcuaga       60

gaugacgaua auaaccucau ccgggucaaa ggcaagaaug uuauuggggc ccgccuguuu      120

ucugagggaa aaacagcgca ggaaagacgg aucagccgca cguccagacg gcgcuauaac      180

agacggaagu ggcgccuuag acugcucgac gagauuuucc augaugcuau cgugcacaaa      240

gacgccgaau uuuucaaucg gcugaagcaa ucuagccuuu cccccaagga ugagcgcaaa      300

caguacuuug gcucucuccu guucccugaa gugggggacg gagauuauuu uagagacggc      360

aaccagacca ucuaccaucu gcggcaccgc cugaugacac aggaugagaa ggccgacauu      420

agagaggucu aucucgcucu gcggcauauc auuaaauaca gaggcaauuu ccuugaugac      480

accccgguga gcuccuuuga agccagcgac cugaagcucg cuucuuucgu ucccacacug      540

aacgaucuuu uugagaccau caaugugaac uaugaacugu ccauugacaa ugucaaccaa      600

aucgagaaua uucuccugaa caauaaacug cgcaacagcg auaagucuaa gcagcucaca      660

gaccugaucg ccgagccugu gaccaacauc gaugacaaau cccuuaagaa acaguucgau      720

aaggaacgga aagacauuag caaggagauc gcgaaagccg uugugggguc uaagaugaau      780

gucaacaagc ugcucuccuu ugaaagcucc gauggaucua aaauuagcuu cuccuucagc      840

gacgcuucuu ccgaggauaa gaugcuggaa cuuagcgaca uccuggacga uucuagaaug      900

aauauucuca acgugcugaa agugaucuac agccgcgucc ggcugaauca gaucauuccc      960

uccggcaugg ggcucucuga gagcaugauc gccgcuuacg accugcacaa gcaacagcuu     1020

aaagaucuga agacgcuccu ggacguugug ccuuuuaacg agaaguaugc cgugaaaauu     1080

gcguacgccg cuuauaucgg aaauauugau aagcagagau ucaccaaagc cgaagucaag     1140

acaaucgaca aacuucgcga uaagaagucc aucaccagcg ugcuggacuu uaaaucuggc     1200

cacauuacaa acgaggaacu caauaacguu aucaaggagg ugauugucga uaaauaccug     1260

aaugacgaaa ccuucgagua ucaugauauc guggagaaga uuaaccagga caucuccaau     1320

gggaacuacc ugcucaaaca acggaauaac cagaacggaa auauucccca ccagcugcau     1380

caaauggaac uugaucagau caucgagaag cagagcaagu auuacccuuu ucugggcaaa     1440

cucaacccca auucuaagag aucccacauu gcuaaauaca agaucagcga acugguugcc     1500

uuccgcgugc cguauuacgu cggcccucug auuacgcccg aggaacagga gaaaacaucc     1560

ggggccucuu uugcuuggau gaagcggaaa gaggacggag cgaucacccc uuggaacuuc     1620

gaaaagaugg uggacaagga gagcacagcc uccagauuua uuaaacgccu uaccgucaag     1680

gauaccuauc uccugaauga agacgugcug cccgauaacu cucuccugua cgagaaauuc     1740

aagguucuua augagcugaa cguggucaaa gcuaauaaca agcggcucac aguggaagag     1800

aagcaaggcg ccuuugacaa ucuguucaaa cagaugaaga cgguuaccgc caaaagacug     1860

aagaacuauc uugugagcaa ucacaaccau aucaauuccg ucaaaaucac agggcucagc     1920

gauggagaaa aguuuaacaa cucucugucc guguacauug acuucaagaa aaucuucggc     1980

gauaagauug acgauagcaa acuguuugac gauuucgaga auaucauuga auggucuacc     2040

guguuugagg accgggauau cuucgaggaa aagguucgca aagacaucuc cuggauuagc     2100

gagucugaaa ucaacgcugu cgugagcaag agauauuccg gguggggacg gcucagcaag     2160

aaacugcuua auggcauuac aaacgacgag ggcagaaaua ucauugauga acugugggag     2220

gagucugaaa ccuuuaugga ggccgugucc aagccggaag ucaaagagaa gaucgacgaa     2280

aucaacagca auuucauuaa aaacacggau aucgaguccg ugcucgcgga cgcuuacaca     2340

ucuccucaga auaagaaagc cauucgcgag accuacaagc ugguuaagga uauccagaaa     2400

gccaugggag ggcaagcucc cagcuccauu ucuaucgaau uuacccggaa cccucaggac     2460

aagggcgagc ugacaagaag ccgccacucc gaaauugcca aaaaguauaa agagcuuucu     2520

aaggagguga gcaagaaacu caaugaugaa cugaagucca aaaagaacag cauggacguc     2580

gauaaauacu aucuguacuu caugcagaug gggaucgacc ucuaugaugg aaccucuauc     2640

gacauugacg aucugaauaa cuaccagauc gaccauauug ugccccaauc cuuuuauaag     2700

gaugacagcu ucgauaauag aguucuuaca cacuacucua acaaccagaa gaaaggcaau     2760

aacacgcccc ugaaugggcu caacaucgac uccaauaccc gggaggugug gaaccaucug     2820

auuggccugg gacuuaucac aaagcgcaaa uacaauaacc ucacccugaa gggcauggau     2880

cagcugagca aauauacaaa gcacggguuu aucagacggc agcucgucga aaccucucaa     2940

gugauuaagc ugguugcuaa uauccuuucc gacgaguaca gcaaagauga cacgaagauu     3000

aucgaaguga aagccaagcu gaacucccag auucgccagg ucuucaaucu cuauaaaauc     3060

agagagcuga acgauuacca ccaugcgcug gacgccuauc uuaccacauu uaucggaggc     3120

uaccuguaua aucgguaccc uaaccuccgc aauauguucg uguacgggga uuucaagaag     3180

uuuagcgcua aaauugacaa ccuggagacc uucaauuuuc uccacgacau cacaaacccg     3240

ucugauaagu ucgaagacag aauuaucgau gagaaaagcg gagaagugau ucuggaccgg     3300

cgccaugcca ucgagaccau uaagaaaauc uaucaauaca acuauaugcu uaucacgaga     3360

gaagucucca caaagaaugg ccagcugucu gaucagaccg uuuacagcgc ucggagaauu     3420

aacaaaguca auucccccau cccugugaag aaggacucua accccgagcu cuauggguuu     3480

cgcacaggca auguggauca ccacauggug auuguuaaau accugaacaa ggacaaauau     3540

gauuacaaga ucauuggaau cccucugcgg uucgugcagg uccuuaaaaa uagcaacgac     3600

aauaccgagu acaagaacau ccugaauaag auugugcaau ccaaacucaa gggcaaaucu     3660

accgauuuua gaguuauccu ggacaacgug auguaugggc agcucauuca ggauggaggc     3720

cagaaguuca cacuggggag cuccacguac aaauauaaug ccaagcaacu ugucaucaac     3780

auggacgaca uucgcauccu gguggauaau cgguacuuua acagccucuc ugacgaugac     3840

gcguccggag aguucaucaa gauuuauaac gaaauccuga aaguuauuaa ugccagauuu     3900

gaucuguucg acaucaacaa guuccgcgaa aaacuuaaua acggcaagga guacuuugaa     3960

aaucugagca uugaugagaa aaaccaggug cuccugaaga ugcucucugg gcugcauugc     4020

ggcuccgcua auguccccau caaggagcuu ggacugacca caccgcuggg caaacuccag     4080

auuccuagcg ggaucgugcu guccgaagac gccaucguga uuuaucaguc ucccagcgga     4140

cuuuucgagc ggaagaucag acugaaagau auuuga                               4176


<210>  4406
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4406
augaaccuga gaaauuaccc cggcgaguau agcaucgggc ucgacauggg aaccggcucc       60

gugggguggu cugucguuga ugaaaaggga aaccugcuuc acuucaaaaa gaaaccuaca      120

uggggcagcc ggcuguuuga cuccgugaug ccggccucug aggcucgcgu gcauagaggg      180

cagcggcgca gauacgaacg gcgcagaugg cggcucgauc ugcuucaagg acuguucaag      240

aaugagauga acaaagucga ccccgaauuu uucaugcgcc ucaaucagag cagaacgauu      300

gagggcgauc cuaucuuuac caaguccuuc acaaaggccg acuauuacga ucgguuuccc      360

accauuuauc accuucgcgc guaccugaug gagucuaacg aaccugcuga ccucagacug      420

aucuaucuug ccaugcauaa uauuaucaaa caccggggca acuuccugcg cgaggaagaa      480

aagagccucu ccgccaaaca ugcugauagc aagaaagccg ugacacaguu uguugacgcu      540

cugaaggagu ggugcuucuc uagaggguac gaauuuggaa aggaucccgc caaauccauc      600

gagaaguguc uuagcgacuc uaaauccagc cggucucagc uggcguccga aauugccaag      660

gcucucgccg ugagcaccga ugcugccgcg gccgacaaaa agcuggcuga caaucuggcc      720

aaagcuaugg ucggccucca augcgaguuc aaguacgugu uuggggaguu cgauugugag      780

uccacaaagc uggcccuuuc ugacgaugag aaacuggaac uccugagagc cgcuugcccu      840

gacgauagcg uugagcuucu ggacgcgcuc ugugccgugu auuccgcuua cguccugcag      900

ggacugcuca gcuaugcccc gggccagacc aucucucaca acaugauugc caaguacgau      960

cuguauaaaa cggaccuuaa gacccugcgc gcucucgugc gggaauacgc ccccggggcg     1020

uaugaugagu ucuuuagagg agcuacauac cacgacgcca auccugauga cuccagcaaa     1080

gacuacucuu augccgaugc ugaaggcuac accgccuaua accugcauaa gcugagcuac     1140

gacgauuucg cuaaggccgu gaaaaagcug cucggcggga cagacgcggu caccgaugag     1200

cgcuaucuga auaugaugga gcgguuugaa aaacagcaau uccugagacg ccucaagacg     1260

uccgacaacg gagccaucua cuaucagcug caccuugagg aacugacagc uauuaucaaa     1320

aaucagggcc gguuuuacuc uuuccucaag gaguacgaag ggaaguuuga gagccugguu     1380

uccuuccgca uucccuauua cgugggaccu cugaccagcg ugaacgccgc ucaggauucu     1440

cauaaccaca auagauuuca augguccgag cggaaacccg gcauggaaag agaggucauc     1500

acaccgugga acugggacac caucauugau aagaauaaaa gcgccgaaaa cuucaucaug     1560

cgcaugaccg gggacugcac auaucuugcg ggagagaagg ugcucccuaa acagucucug     1620

cuguacgagg aauuuugugu ucucaaugag cugaacggcg ugcggugguc cgaugacggc     1680

gaugacuggc agagauucga cgccgcucaa cgggaaggga uuguccgcga gcuuuuucac     1740

aaguauagac gggugacgua caagaugcug gaagauuggc ucguuagaga gggagacgcc     1800

aaucauccca ucgugaaagg cgggcaggga gagaccggcc uggaaagcaa gauggggucc     1860

uaucuguucu ucgcuaaagg cauuuuugga cuuggcgagc ucucuccugc cgaauacccc     1920

gccaucgaga agaugaucaa auggaacaca cuguucgaag aucgcagcau ucugaaggag     1980

aaacucgagg acgaauuugg ggagcgggga aauggcaugc uggaugcuuc ccagaucaag     2040

gcgauuugca agaaaagauu caccgggugg ggacgccuuu cugaacaguu ucugacaggc     2100

cucaaggucg agagccccgc cgggagagcu guguccauca uggacguccu gcgggaaggc     2160

aacccuugua gcgagcgcag acaaggagag accgugguua ugauggaagc ccugcgggau     2220

gagggccuug gguuccagaa aaagauugac gaacacaauc gcaacuacua uggacagcau     2280

ggcaaagauc ucggggugaa ugagcugccg ggaucucccg ccaucagacg gucccugaac     2340

caggcuaucc gcauugucga ggaaaucgcc agcauugcgg gccacgcucc ugccaauauc     2400

uuuauugagg ugacgagagg ggaagacgcc ggcaagaagg gaaaacggac aguuaagcgc     2460

ugggauaaaa ucggcgaggc ucucaagaaa cugaaggaag aggggguggc cgucgaggug     2520

auggacgauu ucaaggaaca aaaagcgauu aacgcuaacc uugacgagag acugucucuc     2580

uaccugaugc agaauggaaa gugccuguau uccggcaaag ccaucaaccu ugaaaagcuc     2640

cuggccggga gcggagagua cgaaguggac cauaucauuc cccgggcuua uaucaaagau     2700

gacucuauug agaauaaggc ccugguucuc agagaggaaa accagcacaa gaccgaucag     2760

cugcuuaucg acaaaagcau ucgcuggaag augcgggaga ccugguccca acugcacgau     2820

gcuggccuca ucggggacaa aaaguaccau aaucugcuga gaagcaacau caaugaaaac     2880

cagcuucgcg gcuuuauugc ccggcagcug gucgagacau cucagauggu gaaacucgcg     2940

caagcccugc ucgaagcuag auucgccaau gaggagaccc gcaucgugcc ugucaaggcu     3000

uccaugagcc acaaccugcg ggaagccgcc gaucuugcua aauguagaga ggcgaaugac     3060

uuucaucacg cccacgaugc uuuccuggcc ucccgcauug gacucuuugu gcagaugcgg     3120

caucccggca ucuaugaaaa cccgauuggg uacucucacg uuaucaagaa guacgugcag     3180

gagcaaagcg aggucuucaa aaagacacau agaaugccug gauccgccgg cuucgugauc     3240

aauucuuuua ugagcuccgg guucgacaaa gauacgggag aaguuuuuaa ggacucuugg     3300

gacgcugagg ccgaagugga uggcauucgg aaagcgcuga acuaucgcca gugcuacauc     3360

agcagaaugc cccagcagga caccggcgcu uucuggaaug ccacagucua uuccccucgg     3420

gaucccaaga uggggccuaa gcuggcccuu agcaccaaac aaggacugga ccccaagcuc     3480

uacggcgggu uuucuuccca gcaguucgcu uacuuuuuca uuuaugaggc caaaaagaaa     3540

ggaaagccga cauuucgcuu cagccaggug ccuguuuggc uggcggcuag aaucgaagcc     3600

aacccccucg cccuggagga guaugcucgg ggccuugcca agggggaagg acuggaguuu     3660

gugucuauuc aaagacccaa aaucuacaag aaacagcuca ucgaagucaa cggcgauaag     3720

cugauuauca ccggcgcuaa agaggugcgc uccggggccg aacuggcguu caaucaggac     3780

gagauuagcg ccaucucuau gugcuccaaa cuucgggcug ccgaggcugg acuggauggc     3840

gccuucagcg aagagguguc cgccauuugg aacagaauca gcaaugcucc uguccagucu     3900

ugugggcgcc uccugcggca acucacgcug ccgaaguacc agagcaacuu ugacuccucu     3960

agcaaugaag agaaggcggc cguugugcuu ggacugauug cucugcucaa cggcaauaac     4020

agaaucgugg aucuguccgg ggucggaggc ucuagccacg ccggcgccau gaugaugucc     4080

uaucccaaac uucuggacga uccuaaggug cccuucuuua ucauugacca gucuguuacc     4140

gggauguucg aacgcaagac acggguggga cucuga                               4176


<210>  4407
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4407
augaagaaaa uccugggccu cgaccugggg accacaagca uuggaugggc ccuugugaac       60

gagaaggaaa auaacgagga acaguccucu aucauuagac ugggcguccg gguuaauccc      120

cucacggugg augagaaaag caacuucgaa aaggggaaag acaucaccac aaaugcugau      180

cgcaccaagu gcagauccgc ccggcgcaac cugcaaagau uuaaacuucg gcgcgaggcg      240

cuggcugacu gucucaagag acacggacug augacagaug agaccccucu gugcgaaaca      300

aauaacucua uuaucuucaa gaccuacagc aaucgggcca aagccgugug ugagaagauc      360

gcucucgacg auuuuguccg cgugcuguuc augauuaaca aaaagagagg cuauaagucc      420

ucucggaaac uuaauacgga cgaugaaggg acccugguug acggaaugga uguggccaag      480

cuccuguaca acgagcagau cacaccgggc gaguaucuuc ugcgccucuu uaaaaagggc      540

aaaaagacca gacccgacuu cuaccugagc gaucugaaag cugaacucga gcggauuugg      600

gacuuucagc gcacauucua uccugaaauc cugugcgacg cccuuaagga ggaucugaag      660

gggaaaucca gaacccagag cucugcgauu uucuacgcca aguauaaagu cuccacggcu      720

aagaucgccg gaaaagacaa gcggcuccaa gaacugacau ggagaaccga ugcccuucgc      780

aagcagcuga caauugacga gguggcuuac guuaucugua gcaucaaugg cgagauuaac      840

gggucuuccc agcggcucag caugaucucu gauagaucca aacugcugua cuucaaucau      900

cagaccauug gacaauaucu caugcagcgc cuggaagaga accccaacgc gggccuuaag      960

aauaucauuu ucuaccggca ggacuaugaa gaugaguuca acgacaucug gaauagccag     1020

gccaccuacc acaaagaacu gacaccugag cucaagaaag agcugaagga uaucaucuuu     1080

uaucaaagag ggcuuaaguc cugcaaaugg cugauuaacu acugugaacu cgagcaugaa     1140

gagauugaag ugaagacgcc cgagggaaaa cugcgcaaga ucaccauugg cucuaaaaca     1200

ugcccuaaga gcuccccgcu guuccaggag uucaagaucu ggcagguccu caauaaccug     1260

caagugagcc cccuuaauuc ucggagaaaa gcuaagcgca aaaaggacga ggaaccuucc     1320

cuguuuagcg augaagacau gaacaccgcc ugucgguauc ucaaugagga ggaaaaacug     1380

acacuguuca aggagcuuuc uaucaaaaag gaacucaaga aaaaggacgc ucugaaacug     1440

cucuuugaga acuacaagga acuggaucuu aauuacaaag ugauuagcgg caacgucacc     1500

auggccuccc uguauagagc cuuccaggac aucauuucuc ucacggggaa uggagaguuu     1560

gauuuccagg cuaugcccgc ggaggaagcc gagaagacag uugaagagau cuuuggcggg     1620

cugggauaca agaccgcuuu ccugcacuuu gacgccagcc uucauggcaa agaauuugag     1680

cgccagccug ccuaucggcu cuggcaucug cuguacuccu augcugggga ucaaagcaag     1740

accggaauug aggcccucgu gaaaagaauc caggaacugu gcggcuuuga gcccgaauac     1800

gcgcggauuc uugcuaacau cucuuucauc ccggacuaug gcaaucuguc cagcaaggcc     1860

augcgcaaaa uucucccuua caugcaggau ggguacgagu auucugaagc cuguuccaag     1920

gcuggauaca gacacagcgc cuccucucug acaaaggagg agaucaacaa aaagaccuau     1980

aaaacacacc uggacgaaau uaagcggaac agccuuagaa aucccaucgu ggagaaaauu     2040

cucaaccaga uggucaaugu gguuaacucc gugaucgaua cguacggcuc uaucgacgaa     2100

auucgcaucg agauggcucg ggagcugaaa aagagcaagc cugaaagaga ggauaugucc     2160

uuugccauua auaaggcgac caaagaaaca gagcgcauca aggggauucu ggaacaagag     2220

uucaaaaucc agcacgucac cagaaacgac aucauucggu aucgccucua cgaggaacug     2280

aagcccaaug gauauaaaac acuuuacagc aacaccuaca uccccagaga ggaucuguuc     2340

ucuaagaagu uugacauuga acauaucauu ccuaaagccc gccucuucga cgauuccuau     2400

agcaauaaga cgcuggagcu gcgggacauc aacaucgaaa aaggcaauca gaccgcuuac     2460

gauuuugugc uuucuaagua uggggaggag ggccucuaca aauaugaaca gagaguugag     2520

gaauuuaaga agaacaauga gcaagccgga uccaaaugcc ggaagcugcu gaugcgcgaa     2580

gcugacauuc cgagcgauuu ucucaacaga gaccugaaug auucucagua caucgccaaa     2640

aaggcgcacg agcuucugcg ggagauugug cccacaguca ccgccacaac cggcagcauc     2700

acgaaccgcc ucagagaaga cuggaaacug gugaacguca ugaaggagcu gaauugggaa     2760

aaguaugaua aacuugggcu gacagaggag uacaaggaca aagauggaaa caccgugcgg     2820

cacauuaagg acuggacaaa aagaaaugau caucgccacc augcuaugga cgcccucacc     2880

aucgcuuuca ccaagccuuc cuuuauucag uaccugaaca aucucaacgc ccggagcaau     2940

aagggcgggu cuaucuaugg aaucgaacag aaagagcugg aaagagagaa caauaaacuu     3000

guuuucauuc ccccuaucga ccccaagauu uuucgcaagc aagccaaaga acaccugucc     3060

aacauccuca uuagcaucaa ggcuaagaau aaagugguca caaagaacgu gaauaaaacg     3120

aaguccaaau cuggcaccaa ccggaagguu cagcugacac cgagagggca gcugcaucuu     3180

gagaccgugu acggcaaaau ccgcacauau aagaccgagg aaauuaaggu cggaacgaaa     3240

cugacagagg aacucaucgc gaagguggcc gauaaaaaga uucgggaggc ucugaugcaa     3300

agacucagcg aauacggcgg gaauaucaaa aaggccuucc agaacauuuc caagcacccu     3360

aucuggcuga acucuagcca caccaaacau gugcccgaga agguuaaaau cgucuuuuuc     3420

gaggacgugu uuaccauucg caaggccauc acaccugaua uuaaaaucga caaggugauu     3480

aauuccaaga uccggaacau ucuucaggaa agacugaaug aguucggagg cgaagcugcc     3540

aaagcguucu cuaaccucga ucagaauccc aucugguuua acaaggacaa agggaucgcu     3600

auuaagagcg ucaaaauccg gggaauugcc aauggcaccg ccauccgcgu gaagaaggau     3660

auucacggga acacaaucau cggcaauaac ggauauccua uucccaauga cuacguuaac     3720

acgggcaaua accaucacgu ggcuaucuuc agagaucggg acgggaaucu gcaagagcac     3780

auugucuccu uuuaugaagc caccgcgcgc gcuauccaga accugccgau uguggauaga     3840

aauuacaaca gcgagaaaga cugggaguuc cuguuuucua ugaagcagaa cgaauacuuc     3900

guuuuuccua aucccaacac aggauucaau ccccuugaga ucgaccuccg gaacgaagag     3960

aauuaugccc ugaucucccc uaaccucuuu agagugcaga aacugagcca uuacuauuac     4020

gucuuccgcc accaucuuga gaccaaugug gauacaaacc ugcaacugaa ggacauuacc     4080

uggaaacgga ucaaggccau uaauaaccuc acgggcguug ugaaagucag aaucgaucac     4140

cuggggcgca uuguggacgu gggagaauau aaguga                               4176


<210>  4408
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4408
augaagaaaa gaaucgacga uuacauucug ggccucgaca ucgggaccaa cagcugcgga       60

ugggccguga cagauaagaa aaauaaccug cuuaagcugc ggggcaaaac ggcuauuggg      120

ucccaccucu ucgaggaagg acauaccgcc gcggaccgca gaggcuuucg gacaacccgc      180

agacggcuga agcgcagaaa guggcggcuu cgccugcucg aggaaaucuu cgcugagccc      240

auggccgaag ucgauccugg guuuuucguu agacugcacc agucuugggu gagcccgcug      300

gacaaauccc ggaagaaaua ucaagccauu guguuuccca cagcuaagga ggaucaggag      360

uacuaucaga aauacccuac caucuaucau cuccgcgacg cccugaugac acaggauaga      420

caauucgacc uucgggaaau cuuucuggcu auucaccaua ucgucaagua ccgcggaaau      480

uuccuccagg auaccgccgu gaaggacuuu gaggcgucua aaauugaagu uggccccauc      540

cugacgaaca uuaauaacgc cuucgcugau aagaucgugg agggccagga ccuuauugag      600

cugaaugucg ccaacgcugc cgaaaucgag gcggugauca gagggaaaga cgccgauaag      660

acccucuaua aaauggacaa gguuaaaaag auugcuaagc ugcugacaga uagcaccgcc      720

aaagaacaga agaauguggc uaaacaaauc gccaacgcca uuaugggaua caagacacag      780

cucgagacca uccugggcaa agaaauugac aagaaugaua aggcucagug ggaguuuaaa      840

cuuuccgacg cggaugacaa gcuggaugcc cuccugccug accuugauga gucugaccag      900

acggucgugg cugaaaucga gaaacuguuc agcgccauca cacucuccac cauuguggac      960

gaaaacaaga gccugucuca auccaugguc gagaaauaca auaagcacca ccaggauuau     1020

aagcugcuca aagccuacau cgaaagccug cccgaccggg cucaggccaa gaaacuucug     1080

cucgcguaug aucuguacgu uaacaauaga caugggcacc uucuggaggc uaagacagag     1140

uuuaaacaag acucuacccu caccaaggaa gauuucuaua agacagugaa agacaaccug     1200

gaugacuccg agcuggccca uaagauugcc caggaaaucg cugccgauaa cuucaugccu     1260

aaacagcgca cgaauagcaa cggagugauu ccguuucagc uccaccaaau cgagcuggac     1320

aagaucauug cuaaucaguc uaaauacuau cccuuccuug ccgcggaaaa cccugucgag     1380

gaucaccgga agcaggcccc cuacaaacug gacgagcucg ugagauuucg cguuccuuac     1440

uaugugggcc ccaugaucac cgcugaugaa caggagaaga cauccggaaa gagcuucgcc     1500

uggauggucc ggaaagaaca agggcagauu accccgugga auuuugagca gaagguggac     1560

agacaggagu ccgcuaacaa auucaucaag cgcaugacaa uuaaagacac cuaccugcug     1620

ggcgaagaug uucuuccugc caauucucuc cuguaucaac gguuugaggu gcugaacgaa     1680

cucaauaaca ucagagucaa uggcagccgc auuuccgugg accugaagca gcagaucuuc     1740

aacgaucuuu uugaggaaaa gaaaacgguc acagagaagg cgcugaucag cuaccucaaa     1800

cagaaucugc aucugcccac cguggagauu aaggggcuug ccgacccuac caaauucaac     1860

ucuucccucg cuagcuauua ccaccugaag aaucugcaug uuuuugauaa ggaacucgcc     1920

gacccccaau aucagaaaga guucgaaaag aucauugagu acucuagcau cuuugaagau     1980

aaaaagauuu uccaggacaa acugcacgcu gaguucaagu ggcuuacacc cgcccaauuu     2040

aaggccaucu ccaccuggcg gcugcaggga uggggcagac ucucucgcaa acugcuggug     2100

gagcuucaug auacaaacgg gcagaacauc auugaacagc ucugggacag ccaaaagaau     2160

uucaugcaga ucgucacgga gccugauuuu aaagaagcua uugcgaagga gaaccagaau     2220

aucacccagg aaaacggagu ggaggagauu cuggccgacg cuuacacauc cccggccaau     2280

aaaaaggcca uccggcaagu ugugaaaguc guggcugaug ugguuaaggc cgcgagcggc     2340

aaggcucccg cccaguucgc caucgaauuu accagagacc cugacaaaaa cccccagcug     2400

ucucacauuc gggggacaaa gcuccugaag gcuuaucagg agaccgccgg agaacuuguc     2460

gaucaaaauc ugacgaacuc ccucaaggag gcgaugacca gccgcaaacu gcugaaggac     2520

aaguacuucc uuuauuuuau gcagacaggc agagaugcuu acaccggcaa aaagaucaau     2580

auugacgagg ugucuacaaa cuaucagauc gaucacauuc ucccucaauc cuucaucaaa     2640

gacgauagcu uugacaaucg ggugcugacc gccacgcccc ugaacgccga aaaguccgau     2700

gacgucccgu acaaaagauu cgcuaauaac uaugugucug aucucaagau gacaguuggg     2760

gagaugugga agcauuggca gaaagccgga aucauuaaua agcacaaacu gggcaaccuu     2820

cugcucgacc cugaccgccu gagcaaguuu cagaaauccg gguucaucaa ccggcagcug     2880

guggaaaccu cucaaauugu caaacuugug agcguuaucc ugcagaauaa guaccccgau     2940

gcugagauua ucacagugaa agccggagac aacuccgcgc ucagacagcg ccuggaucuc     3000

uacaagagca gagacgucaa ugauuaucau cacgccauug acgcuuaccu gucuaucgug     3060

uguggcaacu uucuuuauca gguuuacccu aaauaucggc ccuacuucgu guaugggcaa     3120

uucaagaaau uuucccagga uccugaccug cagaaggauc ucaucaagca cuucaaaggc     3180

uuuaccuuca uguggccccu gcugcagaag gacaauagcg aacgcaaagc ccuugaggag     3240

auuaaggaaa acaauucuga uagaaucguc uuuuacaaac auccggacau uuucgauaag     3300

cugcggaagg cuuacaacua uaaauacaug cucguguccc gcgagaccag cacagaaucu     3360

agcuccaugu uuaauaugac gaucuauccu agaggagagc gggaccgcaa gaaaaccaga     3420

aagcugauuc ccaaaggcaa cgggcucgac cccaagaucu acggaggcua uagcgggaau     3480

acagaugccu acauggugau cgucaaaauu gaaaaggcca aggagucuau cuauaaaguu     3540

auuggaaucc cuaugcgggc ucuggcgggc cuuaagcaag ccaaaaacca ggggaauuac     3600

aaggaggaac ugcacaaagu gguggagccg cagauuaugc ucgacaagaa cggcaagccc     3660

aaacgcggag ucgaaggcuu cagagugguu aaggaucaug ugccuuuuaa acaggucgug     3720

cuggacgggg auaagaaauu caugcuguac uccgcuaagu augagaccaa ugccaagcaa     3780

cuuacacuga gccaggaaac caugcggauc guuacggaca accucaaaaa guccgaggau     3840

caggacaaac ugcucgugaa ggccuacgau gagauccugc aaaaagucga ccaguaucuu     3900

ccccuguuug augugaacaa guucagaaau ucucugcacc ucggacgcgc uaaauuccug     3960

gaccuugccg uuaacgauaa gaagauuaca cugagcaaua uccucaacgg ccugcaugac     4020

aaucucgugg ucccuaaccu gaaaaauauu gggaucaaga cccccuuugg acuuaugcag     4080

uccccuucug gcauuacccu gagcuccaac gcgauccuga uuuuccaguc ucccacaggg     4140

cucuuugaaa aacgggugag aaucgcugac cuguga                               4176


<210>  4409
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4409
auggagaaca agcaguacua uaucggccug gacgugggga ccaauagcgu cggaugggcc       60

guuacagaua cguccuacaa ccuccugaga gcuaaaggca aggacaugug gggagcccgg      120

cuuuucgaug aagcgaaaac cgcuguggag cgcagaacaa agcggaccuc ucgcagacgg      180

agcgaacgcg agaaagccag aaaggccaug cugaaggaac ucuuugcuga cgagauuaau      240

aaaguggauc ccaacuucuu uauccggcug gaggaaucca aguucuuucu ugacgaucgc      300

ucugagaaua acagacaacg guauacacug uucaaugacg ccaaauuuac cgauaaggac      360

uacuaugaaa aauacaagac aauuuuccac cuccgcagcg cucugaucaa cuccgaugag      420

aaguuugacg ucagacuggu guuccucgcc auucugaauc uuuucucuca ucgggggcac      480

uuccugaacg cgagccucaa aggcgauggg gagauccagg gaguugacau cuuuuauaau      540

gaccuguacg aagugugcga ggaauuugag auugaucuuc cucagaucuc caacauugac      600

aauuucgaaa agauccugag ccagaaaggc aagucucgca ccaaaauucu cgaggagcug      660

uccgaagaac ugagcaucuc uaagaaagaa aagauuaagu acucccuccu gaaacuuauc      720

uguggcauga agaucaaagu cguggauauu uaugccgacg aggauaucca agaagacgau      780

aagaaaaagg accugggguu uagagagagc gauuacgaga agucuucccu cgaaguuaaa      840

gagauuaucg gagacgauca uuucgaacug auugagcggg cuaaggccgu gcacgacauc      900

ggccuucugg cuaacaucau ugggaauagc aaauaucucu gcgaagccag agucgaggcg      960

uacgacaacc aucacaagga ucugcagaaa aucaaggagc ugcucaagaa auaugacauu     1020

aaggccuaca cggauauguu ucgcaaaaug accgacaaga acuauuccgc uuacguggga     1080

ucugugaaua gcaacaucgc caaagaacgg agauccgucg auaaucgcaa gauugaggac     1140

cuguacgaau auaucaagaa aaucgcucuu aagaacauua gcgaugacaa ugaggauaaa     1200

aucgagauuc ugaagaaaau cgaaacaggc gaguuccuca agaaacagcu gaccgccucu     1260

aacgggguua uuccgaauca gcuucaaucc cgggaacuga gagccauccu caagaaggcu     1320

gagaacuacc ugcccuuucu gaaagaaaag ggagagaaaa aucucacagu gagcgagaug     1380

auuauccagc uguucgaauu ucagaucccu uauuacgugg gcccccuuga caagaacccu     1440

aaaaaggaua agaaugcgaa cucuugggcc gucauuccca gcggcgggcg cauccugccu     1500

uggaauuucg aggacaaagu ggauguuaag ggaucccgga aagaauuuau ugagaagaug     1560

gugagaaaau guaccuaucu cucugaggag cacacgcugc cgaagcagag ccugcuuuac     1620

gagaaguuca ugguccucaa cgaaaucaau aacauuaaaa ucgacggcga gaagaucucc     1680

guggaagcua aacaaaagau uuauaacgac cuguuuauga aagggaagaa gguuagccag     1740

aaagccauca agcaggagcu gauuucucuc aauaucaugu ccaaagauag cgugcugucu     1800

ggaauugaca cagucugcaa cgccuaccuu uccagcaucg cuaaguuccg cggcguguuu     1860

gaugaaaaug ucaacaauca auccaccauc gacaugauug aggauaucau uuuccugaaa     1920

acaguguacu cugacgagaa gcgguucguu aaagaagaga ucguggaaaa guauggggau     1980

gagauugaca aggauaaaau caagagaauc cucggauuua aauucagcaa cuggggcaau     2040

auuuccaagu cuuuucugga gcuggaaggc gccgacgucg ggaccggaga gauccgcagc     2100

auuauccagu cccuuuggga aaccaacuuc aaucucaugg agcugcugag cucucgguuu     2160

acauacaugg augaacucga gaaaagagug aagaagcugg agaaaccccu uuccgaaugg     2220

acgauugagg accuggauga cauguaucuc agcucuccug uuaagcggau gaucuggcag     2280

uccaugaaaa uuguggacga aauccagacc gucaucggcu acgcgcccaa gcgcauuuuc     2340

guggagauga caagaagcga aggggagaaa gugcggacca agucuagaaa ggaucgccug     2400

aaagagcugu auagcgcuau caaggaagac uccaaacaau ggguuaagga gcuugaugcc     2460

aaagacgaaa gcuacuuucg gucuaagaag auguaucucu acuaccugca gaaaggaaga     2520

uguauguauu ccggcgaggu cauugaacug gauaagcuca uggacgauaa ccuguacgac     2580

aucgaucaua uuuauccucg cagcuucgug aaagacgauu cccuugacaa ucuggugcuc     2640

gucaagaaag agaucaacaa uagaaagcag aacgacccca ucacaccgca gauucaagcc     2700

ucuugccagg gguuuuggaa ggugcugcac gagcagggcu ucaucagcaa ugaaaaauac     2760

ucccggcuga cccgcaagac gcaggaguuu ucugaugaag agaaacuuag cuucauuaac     2820

agacaaaucg uugagacagg acaggcuacc aaguguaugg cccagauucu ccagaaaucc     2880

augggcgaag acguggaugu cguguuuucu aaggcucggc ugguuagcga guuccgccau     2940

aaauucgaac uguuuaaguc cagacucauc aacgacuucc accacgccaa ugaugcguau     3000

cugaacaucg uggucgggaa uagcuacuuu gugaaguuca cccggaaccc ugccaauuuu     3060

auuaaagacg cucgcaagaa ccccgauaau ccugccuaua aauaccauau ggacagauuc     3120

uuugagcggg auguuaaguc uaaauccgaa guggcuugga ucggacaaag cgagggcaac     3180

gccgggacaa uugcgaucgu caagaagacc augucuaaaa auagcccccu uauuacaaag     3240

aaaguggagg aaggacacgg cuccaucacg aaggagacca uugugggggu caaagaaauc     3300

aaguucggcc gcaacaaggu ugagaaagcc gacaagacac ccaaaaaggc uaaucugcag     3360

gccuacagac cuaucaaaac cucugaugaa cggcucugca acauucugag auauggaggc     3420

cgcacaucca ucagcauuuc cggguacugu cugguggagu augugaagaa gcggaaaacc     3480

aucagaagcc uugaggcuau uccggucuac cugggacgca aggacucucu cagcgaagag     3540

aaacugcuca auuauuuucg guacaaccug aaugauggcg ggaaggacuc cgugucugac     3600

aucagacuuu gccugcccuu caucagcacg aacucccucg uuaaaauuga uggauaucug     3660

uacuaccugg gcgggaagaa cgacgaucgc auccagcuuu auaaugccua ccaacugaaa     3720

augaagaagg aagaggugga auauauucgg aaaaucgaga aggccgucuc uaugagcaaa     3780

uuugacgaga uugauagaga aaagaacccu gugcucaccg aggagaaaaa uaucgaacug     3840

uacaacaaga uccaggacaa guucgagaau acaguuuucu ccaaacgcau gucucucgug     3900

aaguauaaca aaaaggaucu gagcuuuggc gacuuccuua aaaauaagaa guccaaauuu     3960

gaagagauug aucuggaaaa gcaguguaaa guccucuaca acaucauuuu caaucugagc     4020

aaccugaagg agguggaccu uucugauauc ggaggcucca aaagcaccgg gaagugccgg     4080

ugcaagaaaa auauuacaaa cuauaaggaa uuuaaacuga uccagcaauc uauuaccgga     4140

cucuacuccu gugagaagga ccugaugacg aucuga                               4176


<210>  4410
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4410
augaagagaa acaaugacua cuauaucggc cuggauaugg ggaccaacag cgugggaugg       60

gccacaacga auccccaggg ccggcuccug cgcuucaaca aaaaggacau gugggggucc      120

agacuuuuug aggaagcuaa aaccgccgcg aagacacgga ccuuccgcac aucuagacgg      180

cgcuacgaua gaguccggca acgcauugag uuucugcagg acaucuucgc uccuauuauc      240

gccaaaaagg auuuugaguu cuuuaugaga cucaaggaga gcaaauucca ccuggaagac      300

aagaaaaccg auggaaaguu ugcccuuuuc aaugacgcug auuuuacaga cguugaguau      360

uucgaucggu acccgaccau uuaucaucug cgcgccgcuc ucaucaaacc caaccacauc      420

acggacccua gacugcugua ccucgccauu caucacauca uuaagaaucg gggccauuuu      480

cuguucgcgg ggaccgauau ggaguccauc cgcucuuucc agauugugua uacacagcuu      540

agcgaacacc ugaaguccau caaccucgac uuugagcugc ccguggacgc ccaagauaaa      600

uucgcugcca uucuucugaa ugaaaagcuc aacaaaagag acaagaaaaa gcagcugaaa      660

gagcugugcc cucacaagga uaagcggcuc gaguacgcuu uuaaugccau caucggaggc      720

aaaaccaagc ugaaagacau uuucggcguc gaagaguacg augaagggac aaagaaagcg      780

guggacuuug agaaggccga uuaugacgaa gauaaggacg agaucgaguc uguuauuaaa      840

gaaaacaucc augugcuuaa ugcucugaag gccgucuacg auuggaccau ucucacgaac      900

auccugggag acuaugcuua caucagcgag ucccaggugg cccuuuauga agcccacaaa      960

aaggaccugc gcauucucaa aagccugauc aagaaguacg cuaaaucuca guaugcggau     1020

gccuucagac augagaaugu uaagggcaac uacgcuaacu acgugggguc cacaaauuau     1080

gacaacaaac aacccgucau ugauaauccu agcgugaccc aggaagacau caacaaguac     1140

uuugccgaua uucugcucuc uaucacaccg gacgaguccg aucgggagag cuaucaguac     1200

aucaccgaaa gacugcacaa aggagaggcc cuucccaagc ugcgcaccaa ggcuaauucu     1260

gugcucccuc accagauuca uaaaaucgaa cuggagaaga uucuugccaa cgcggcuaaa     1320

aauuuccccu uucugcaaga aaaggacucc agcggcuaua cagccgccca gaaaaucguc     1380

aagauuauga uguuccggau cgaguacuau guugggccuc ucaacacgua ccaccaugug     1440

gaucagggag gccacgcuug gauggugaga aaagccgagg gcaagauuac ccccuggaau     1500

uuugacgaaa aggucgauaa agaggcuucc gccgaaaagu ucauccgcaa caugacaaau     1560

aaauguaccu accugaucgg gaaggacgug auuccgaaac aaucucugcu cuauagccgg     1620

uacaagguuc ugaacgagcu uaauaaccug aaggugaaug gagcgccuau cuccgucgcc     1680

cucaaacaga agauuuauga ggaacuguuu aaaaagcaag ugaacguuac auucaaccag     1740

cugaaaaccu uccuuaagac gcagggcgag gaugcugacg aaaucauucu cagcggggug     1800

gacgaggccg cucauaaguu uacaaauaac cugcagaccu accacaaauu cagagccauc     1860

cugcgcgaua agaucgaauu ugagccccag cggucuaugg ucgaggacau uaucagaugg     1920

aaaugccuca ugggagauga cauuaccaug uggaagcaca aaaucgaaaa ggaguauggc     1980

gaucagcugu ccgaagaaca acuuaagaaa auuacacgcc ugaauuucaa cggguggagc     2040

cggcucucuc aggaauuucu gaccgacgug ccuggagucg auaucgagag cggcgaggug     2100

uacuccggca ucauggacgc gcuguggaga acaaaugaaa acauuaugca gcuucucucu     2160

agccgguuca cguuugccgc ugagguuaag aaacagaauc aagggaccgg acgcaucgaa     2220

aagauuacau augaugaggu gaugaaagac accuaccugu cccccgccgu caagagaagc     2280

guguggcagg cuaucacacu gguugaagag auucggaaaa ucagaaaggc cgaucccaag     2340

cgcaucuucg uggagaugac ccggcagccu gacgccaaaa aggaaagaaa acucucucgc     2400

agaacgcagc ugcuuaacgc uuaccgggcg auuggcaaug auaucgccga guaugaaaag     2460

gagcaugucu ccacccugau uacacaacuc gagagcaaaa aggacgaaga ccugcgcaga     2520

aagaaacugu accuuuauuu uacccagaug gggcggugua uguacacagg agagcgcauc     2580

gaucucucuu cccugcugag agacaccaac agcaauauuu augauaucga ccacauuuac     2640

ccgcgguccc ucacgaagga ugacucucug gauaacacag ugcuuugcaa aaaggaaauc     2700

aaucugaaaa agggcaacaa guauauccau cgcguguuca ccgacaaaga gcucaagaaa     2760

caguacgggu acuggaaggc ucugaaagaa aagaagcuga uuagcgagag aaaauuuucc     2820

cggcuuaugc gcacaaccga ucucaccgcc gacgagcugg ccggcuucau cucuagacaa     2880

cugguugaaa caagccagau ggucaaggcu acggccgaua uucucgagaa aaucuauccc     2940

aaugugacca uuguguacgu caaggcgcgg acaguguccg acuuuagaca cgacuaugaa     3000

cugccuaaag uucgcgcucu uaacgauugu caccaugccc acgacgccua ccugaacauc     3060

guggucggaa auguguauua cgagaaguuc accaagagcc ccuuccgguu uaucgcuaac     3120

cagaaaggca agggggauau uaaauauaau cucuacagac uguucgacaa ggauguuaaa     3180

gugggagacc gcaucauuug gaacgccaag gaagaucugg cgcgggucaa agcuacacuu     3240

gagagagaca ccgugaaugu ugugcgcaug ccugucgagc agcggggcca auuuuucgau     3300

gccacgaucc agaagaaggg gacaaccaaa aacaugcugg ugcucccccu gaagggaucu     3360

gacgaaagac uccaggauac cuccaaauac ggcuauuacg ugcgcccgag cgggaugucu     3420

uuuauggucg uucagcauga ggugaagggc aaaucccggg ugacaauuga accugcccug     3480

cuugucuaua agaauaagcu gaaaagcgag gacgauuacg cuaaguauug cgaagacgug     3540

cucaaacugc aaaagcccaa aguucuguuc aagaacauca aguuuggauc uaaaauccuu     3600

uucgacggcu acccuuuucu gcucaagggg agaaccgaga aucggauuau cauggccgau     3660

gcucagagcc uguuccucgg aaaccaggag cugaaaauuc uuaagcgcgu gacaaaaauc     3720

uccgacgaua uugaccacaa ggucgaaagc agaaucaaga uuacggauga ggaauggacc     3780

acacugugga aaucuguggu ugagcagauc gugaccggca aguuucggaa ucgccucggg     3840

acacugaacg gaauccugca aggcagaaaa auuaaacuuu ccaaggacga ggaagcccgg     3900

cugaaugagc ucgauagcaa ggaccaggaa accuuccugu cccagauccu ccuggagaga     3960

uuccuucaau uuucuacgga agagaaaacc cgccugcugc uccaggucau uagcuucucc     4020

gggaacacaa ccggcgugga ucugcggcuu aucggaggcu cuagcgggac aggaaccauu     4080

aagacaaaua agaacaucac cgaccauucc guuauccuga uuaacgaguc ucccacgggc     4140

cucuuuacaa auagaaccgu gcugaaccag ccuuga                               4176


<210>  4411
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4411
augaagagaa acaaugacua cuauaucggc cuggauaugg ggaccaacag cgugggaugg       60

gccacaacga auccccaggg ccggcuccug cgcuucaaca aaaaggacau gugggggucc      120

agacuuuuug aggaagcuaa aaccgccgcg aagacacgga ccuuccgcac aucuagacgg      180

cgcuacgaua gaguccggca acgcauugag uuucugcagg acaucuucgc uccuauuauc      240

gccaaaaagg auuuugaguu cuuuaugaga cucaaggaga gcaaauucca ccuggaagac      300

aagaaaaccg auggaaaguu ugcccuuuuc aaugacgcug auuuuacaga cguugaguau      360

uucgaucggu acccgaccau uuaucaucug cgcgccgcuc ucaucaaacc caaccacauc      420

acggacccua gacugcugua ccucgccauu caucacauca uuaagaaucg gggccauuuu      480

cuguucgcgg ggaccgauau ggaguccauc cgcucuuucc agauugugua uacacagcuu      540

agcgaacacc ugaaguccau caaccucgac uuugagcugc ccguggacgc ccaagauaaa      600

uucgcugcca uucuucugaa ugaaaagcuc aacaaaagag acaagaaaaa gcagcugaaa      660

gagcugugcc cucacaagga uaagcggcuc gaguacgcuu uuaaugccau caucggaggc      720

aaaaccaagc ugaaagacau uuucggcguc gaagaguacg augaagggac aaagaaagcg      780

guggacuuug agaaggccga uuaugacgaa gauaaggacg agaucgaguc uguuauuaaa      840

gaaaacaucc augugcuuaa ugcucugaag gccgucuacg auuggaccau ucucacgaac      900

auccugggag acuaugcuua caucagcgag ucccaggugg cccuuuauga agcccacaaa      960

aaggaccugc gcauucucaa aagccugauc aagaaguacg cuaaaucuca guaugcggau     1020

gccuucagac augagaaugu uaagggcaac uacgcuaacu acgugggguc cacaaauuau     1080

gacaacaaac aacccgucau ugauaauccu agcgugaccc aggaagacau caacaaguac     1140

uuugccgaua uucugcucuc uaucacaccg gacgaguccg aucgggagag cuaucaguac     1200

aucaccgaaa gacugcacaa aggagaggcc cuucccaagc ugcgcaccaa ggcuaauucu     1260

gugcucccuc accagauuca uaaaaucgaa cuggagaaga uucuugccaa cgcggcuaaa     1320

aauuuccccu uucugcaaga aaaggacucc agcggcuaua cagccgccca gaaaaucguc     1380

aagauuauga uguuccggau cgaguacuau guugggccuc ucaacacgua ccaccaugug     1440

gaucagggag gccacgcuug gauggugaga aaagccgagg gcaagauuac ccccuggaau     1500

uuugacgaaa aggucgauaa agaggcuucc gccgaaaagu ucauccgcaa caugacaaau     1560

aaauguaccu accugaucgg gaaggacgug auuccgaaac aaucucugcu cuauagccgg     1620

uacaagguuc ugaacgagcu uaauaaccug aaggugaaug gagucccuau cuccguggcg     1680

cucaaacaga agauuuauga ggaacuguuu aaaaagcagg uuaacgugac auucaaccag     1740

cugaaaaccu uccuuaagac gcaaggcgag gaugccgacg aaaucauucu cagcgggguc     1800

gacgaggcug cccauaaguu uacaaauaac cugcagaccu accacaaauu cagagcuauc     1860

cugcgcgaua agaucgaauu ugagccccag cggucuaugg uggaggacau uaucagaugg     1920

aaaugccuca ugggagauga cauuaccaug uggaagcaca aaaucgaaaa ggaguauggc     1980

gaucagcugu ccgaagaaca acuuaagaaa auuacacgcc ugaauuucaa cggguggagc     2040

cggcucucuc aggaauuucu gaccgacguc ccuggagugg auaucgagag cggcgagguu     2100

uacuccggca ucauggacgc ccuguggaga acaaaugaaa acauuaugca gcuucucucu     2160

agccgguuca cguuugcggc cgaggugaag aaacagaauc aagggaccgg acgcaucgaa     2220

aagauuacau augaugaggu caugaaagac accuaccugu cccccgcugu gaagagaagc     2280

guuuggcagg ccaucacacu gguggaagag auucggaaaa ucagaaaggc ugaucccaag     2340

cgcaucuucg ucgagaugac ccggcagccu gacgccaaaa aggaaagaaa acucucucgc     2400

agaacgcagc ugcuuaacgc cuaccgggcu auuggcaaug auaucgcgga guaugaaaag     2460

gagcaugugu ccacccugau uacacaacuc gagagcaaaa aggacgaaga ccugcgcaga     2520

aagaaacugu accuuuauuu uacccagaug gggcggugua uguacacagg agagcgcauc     2580

gaucucucuu cccugcugag agacaccaac agcaauauuu augauaucga ccacauuuac     2640

ccgcgguccc ucacgaagga ugacucucug gauaacacag ugcuuugcaa aaaggaaauc     2700

aaucugaaaa agggcaacaa guauauccau cgcguuuuca ccgacaaaga gcucaagaaa     2760

caguacgggu acuggaaggc ccugaaagaa aagaagcuga uuagcgagag aaaauuuucc     2820

cggcuuaugc gcacaaccga ucucaccgcu gacgagcugg ccggcuucau cucuagacaa     2880

cuggucgaaa caagccagau ggugaaggcc acggcugaua uucucgagaa aaucuauccc     2940

aaugugacca uugucuacgu gaaggcccgg acaguuuccg acuuuagaca cgacuaugaa     3000

cugccuaaag ugcgcgcgcu uaacgauugu caccaugcuc acgacgccua ccugaacauc     3060

gucgugggaa auguuuauua cgagaaguuc accaagagcc ccuuccgguu uaucgccaac     3120

cagaaaggca agggggauau uaaauauaau cucuacagac uguucgacaa ggaugugaaa     3180

gucggagacc gcaucauuug gaacgcuaag gaagaucugg cccgggugaa agcgacacuu     3240

gagagagaca ccguuaaugu gguccgcaug ccuguggagc agcggggcca auuuuucgau     3300

gcuacgaucc agaagaaggg gacaaccaaa aacaugcugg ugcucccccu gaagggaucu     3360

gacgaaagac uccaggauac cuccaaauac ggcuauuacg uccgcccgag cgggaugucu     3420

uuuaugguug ugcagcauga ggugaagggc aaaucccggg ucacaauuga accugcccug     3480

cuuguguaua agaauaagcu gaaaagcgag gacgauuacg ccaaguauug cgaagacguu     3540

cucaaacugc aaaagcccaa agugcuguuc aagaacauca aguuuggauc uaaaauccuu     3600

uucgacggcu acccuuuucu gcucaagggg agaaccgaga aucggauuau cauggcugau     3660

gcccagagcc uguuccucgg aaaccaggag cugaaaauuc uuaagcgcgu cacaaaaauc     3720

uccgacgaua uugaccacaa gguggaaagc agaaucaaga uuacggauga ggaauggacc     3780

acacugugga aaucuguugu ggagcagauc gucaccggca aguuucggaa ucgccucggg     3840

acacugaacg gaauccugca aggcagaaaa auuaaacuuu ccaaggacga ggaagcucgg     3900

cugaaugagc ucgauagcaa ggaccaggaa accuuccugu cccagauccu ccuggagaga     3960

uuccuucaau uuucuacgga agagaaaacc cgccugcugc uccaggugau uagcuucucc     4020

gggaacacaa ccggcguuga ucugcggcuu aucggaggcu cuagcgggac aggaaccauu     4080

aagacaaaua agaacaucac cgaccauucc gugauccuga uuaacgaguc ucccacgggc     4140

cucuuuacaa auagaaccgu ccugaaccag ccuuga                               4176


<210>  4412
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4412
augaaaagca uccugggccu cgaccugggg accacaucca uuggaugggc ccuugugaaa       60

gagucugaaa acgauaauga aaagagcgaa aucgucaaac ugggcguuag acucgugccc      120

cugacgaccg acgagcggac agauuucgaa aaggggaaau ccauuaccac aaacgcugac      180

cgcaccucua agagaagcau gcggcgcaau cuucagagau acaagcugcg gcgcgaggcc      240

cucguguccg agcugaaaaa ggaacacugg aucgaugagg gauauagauu uucugaagac      300

ggcccuggga gcacauucuc cacccugcgg cuccgcgcga aagcugccga ggaggaaauu      360

agccugcaag aucuugccaa gguccugcuc cagaucaaca aaaagagagg auacaagucu      420

ucccggaaac ugaauagcgc ugaggacggc caggccaucg auggcauguc uguggcuaag      480

guucuuuaug accgcgggcu gacgccggga gaauacgugu aucagagaau gcucgauggc      540

caauacgcca uucccgaguu uuauaaaucc gaccugcagg cggaauuuga uaagaucugg      600

aaccugcaga aaagcuacua cccugagcuc accgccgagc uguaugacaa acuuacagac      660

aaaaauaagu cucagaccug ggcuauuugc cgggaugaac ugggggucca aggauuuaag      720

uccagcuuca caggcaaaga gcgccucaag gaagccuacu uuaacagaca gcaggcuucc      780

aaacagaagg uggaccugga gguucuuacc ugugugcugc aagauaucaa ugggcagaaa      840

gccucucuca gcggacugcu guccgcgauu agcgaccggu cuaaggaacu cuauuucaac      900

agaaugacgg ucggccagau gcuggugcgc caaauccagg aggaucccaa ucggucccuu      960

agaaacacag uguuuuaccg ccaggacuau auugaugagu ucgaaaagau cuggagcguc     1020

cagucucggu uucauccuga gcugacccaa gaaagaaaga agaaacucaa ggacaucauu     1080

aucuucuacc agcgcccccu gaaaagccag aagggccuua uuucccugug cgaguuugaa     1140

ucucgggagg uugauaucag cguggacggg gugauuaaga aaaagaaaau cggaccuaga     1200

gucuguccgc gcuccagccc ccucuuccag gaguucaagg uguaucaaac aguuaauaac     1260

auccugguga auggcgaacc ucuggaugac aacgaccggg agaaacucgu cggggaacug     1320

gccuuuagag agaagaugaa gggacaggau gugcuuaaac ugcucuacaa gaccucuaaa     1380

ggcaugcgca uuaacuucga ggaaguugag gggaaucgga ccauggcuac aauccugaag     1440

gccugcgaga aaauugugga gggacugggc uacgacacga gagaucuuuc caagaucacc     1500

gcuagcgaaa aagucucucg gauugaggag aucuuugcca aggaaggcuu caaguccgac     1560

uuucuccacu ucgauagcuc ccugcccucu ccugaguuug aaagccaggc cuccuauucu     1620

cuguggcauc uccuguacag cuaugagaac gacaaauccg ugagcgggaa ugaaucucuu     1680

gucgagaaga ucaaaaagcu guguuccaug gaugaggcua gcgcgaaaau ucucucugga     1740

ugcacauucc uguccgacua cggcagccug ucugcccgcg cuaugagaaa gauccuuccc     1800

uauaugaagg augggcucga auacagcgcc gccugugcua uggccggaua ucggcacucc     1860

aaaagaagcc ugaccaagga ggaacuggac ucucgcgugu acguugaucg gcucgagcug     1920

cuuccgaaaa acucccugag aaauccugug gucgaaaaga uucucaacca gaugaucaau     1980

gugguuaaca gcguggucga cacauacggc aaacccgaug agauucgcau cgagauggcg     2040

agagaacuga agaaguccgc uucugagcgg gaaaaagccc aaaaggacau ugccgcugcc     2100

agcaaaguga augaguccau cguggcuacc cugaaggggu cuccuuucuu caucgacagc     2160

cccucccgca acgauauuau cagauaucgg cuuugggagg aacucaaacc caauggcuac     2220

aagacgcugu auucuggaac auacauuccu aaggagcgcc uguuuagcaa agacuucgau     2280

aucgaacaua uuaucccgca ggcccuccug uuugacgauu ccuucgcgaa caagaccauc     2340

gagagcagac agugcaauau ugaaaaaucu aaccggaccg ccauggacua uguuaagucc     2400

aaauacgaua gcuucggcgu ccaggcuuau aucgaccgcg ugaaggggcu uacaggagug     2460

ucuaaaacca agauuacaag acugcucaug gaguccaagg auauccccga ggacuuuauu     2520

aaucgggaac ugcgcgauag ccaauacauc gccagaaaag cucgggagau ccuggaaucu     2580

auggucaagu ucgugguucc uacgaccggc uccgugacag ccagacuucg cgaggacugg     2640

cagcucguca acgugaugcg ggaacugaac ugggauaaau acgagaagcu ggggcucacc     2700

agcuuuagag aggaccgcga cggacgggaa auucccguua ucgaggauug gacaaaaaga     2760

aaugaccacc gccaccaugc gauggaugcc cugaccauug cuuucacgcg gagacaguuu     2820

auccaauauc uuaaccaccu gaaugccaag auugacaccg uggauaagac agacaucgau     2880

cuccgcgacu acaaccugga agauaucucc uucgacggcc ugcgggaugg ggacaaauuu     2940

ggcauuguca aggcccuuca gaaaaaguau cuguacaaag acgauaagca gaaguauaga     3000

uucgugccuc ccaugccgcu cgacgaguuu cggaucgaag cuaaacgcca gauugaggau     3060

guucugguga gcuucaaggc caaaaauaag gucuguacca aaaacgugaa uacaaccaag     3120

gugagaggag gccaacagcg gaagauccag cucacgccuc gcgggccccu gcaugaggac     3180

acaauuuacg gaaaaagacu ucaguauguc accaaggaag agaaaguugg cgggucuuuu     3240

gaugaagcua agaucgcgag cgugugcaaa aaguccuacc gggaggcccu gaugagacgc     3300

cucauggagu guggaggcga cccuaagaag gcuuucacag ggaagaacuc ucuggagaaa     3360

aaucccgugu gggucgauga auuucacagc ggcgaagugc cucugcgggu uagaaccgug     3420

uccauggccu cugucuacac cgugcgcaag agcguuggac aaucccuuaa cauugagaaa     3480

gugcuggaca agcacaucaa agaaauccuc cgggccagac uggaggagua uggcgggaau     3540

gcuucuaagg ccuucagcaa ccucgaugaa aaucccauuu ggcugaacaa ggagaaagga     3600

aucgcgguca agcgcgugac aauugacucc ggcaaaaauc cgaucccucu ucggagcaag     3660

cuggauaaag acgggcagau uaucuacgga gaaggccaga acccccagga gucugauuuc     3720

guugugcccg ggggcaacca ucacgcugcc aucuauagaa cgccuuccgg aaagcuccaa     3780

gacgucgugg ugagcuuuua cgaagccguc ucucgcaaga aucugggccu ggagguuauu     3840

gacagaaacu ucaaaaagga ugaggggugg gaauuucuuu ucacccugaa acagaaugag     3900

uauuuugugu ucccgaaccc cgcuacagga uuuuccccug aagacuacga ucucaccgac     3960

cccgccaaua acgcgaucau uagcccuaau cuguaucggg ugcagacagu cuccgugggc     4020

gauuaccgcu ucagacauca ccucgagacc acgguugaag acaacgagaa gcugcgggau     4080

aucgcuugga aacgcauugg gcccucugga cuugugggcg ccgucaaggu gagaaucgac     4140

aaucugggcc ggauccuggc cguuggggag uacgauuga                            4179


<210>  4413
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4413
auggagaaca agaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucaucacag augacuauaa aguucccucu aagaaaauga agguguucgg caauacggau      120

aaacacuuua uuaagaagaa ccucaucggg gcucugcuuu ucgacgaagg agccaccgcg      180

gaggauagac ggcugaaacg cacagcuaga cggcgcuaca ccagacggaa gaaucgccuc      240

agauaucugc aggaaauuuu uagcgaggaa aucuccaaac uugacucuag cuucuuucau      300

cggcuggaug acuccuuccu cgugccuaag gauaaacgcg gcucuaagua cccgaucuuu      360

gccacacugg aggaggaaaa ggaguaucac aaaaaguucc ccaccauuua ccaucugaga      420

aaacaccucg ccgacagcaa ggagaaaaca gaucugcggc uuaucuaucu ggcucucgcc      480

cauaugauua aguaccgcgg gcacuuucug uacgaagagu ccuucgacau caaaaacaau      540

gauauucaaa agaucuuuaa cgaguucauu agcaucuaug acaauaccuu ugaaggaucu      600

ucccuuagcg gccagaacgc ucaggucgaa gccaucuuca cggacaagau uucuaaaucc      660

gcgaagagag agcgggugcu gaaacucuuc agcgaugaaa agucuaccuc ccuguuuagc      720

gaguuccuga aacucaucgu uggcaaucag gccgacuuua agaagcacuu cgaucuggag      780

gaaaaagcuc cucuucaauu uuccaaggac acauacgaug aggaccugga aaaccuccug      840

gggcagauug gagauggcuu caccgaccuu uuucuggugg ccaaaaagcu cuaugaugcu      900

auccugcugu cugggauucu cacagucacc gaccccagca cgaaagcccc ucuguccgcg      960

agcaugaucg agcgcuacga aucucaucag aaggaucuug ccgcucugaa gcaguucauc     1020

aaaaauaacc uccccaagag auauaaugag guguuuuccg accaaagcaa agacggauac     1080

gccggcuaua uugaugggaa gacaacccag gaggcuuucu acaaauacau caagaaccug     1140

cuuucuaagu uugaaggagc cgacuauuuc cuggauaaaa uugagcggga agacuuucuc     1200

agaaagcagc gcacauucga uaacggcagc aucccucacc agauucaucu gcaagagaug     1260

aaugccaucc ugcggagaca gggcgaacac uacccguucc ucaaagagaa ccgcgagaag     1320

aucgagaaaa uucugaccuu ucggaucccc uauuacguug ggccucuugc uagaggaaau     1380

cgcgacuucg cguggcugac ccggaacucc gaucaggcca uuagacccug gaauuuugaa     1440

gaaaucgugg acaaggcuuc uagcgccgag gaguucauua acaaaaugac aaauuaugau     1500

cucuaccugc cugaagagaa gguccugccc aagcacuccc uucucuauga aacguuugcc     1560

guguacaacg agcugaccaa agugaaguuc aucgcugaag gccugcgcga cuaccaauuu     1620

cucgauagcg ggcagaaaaa gcagauuguc aaucagcugu ucaaagagaa gcggaagguu     1680

acagagaaag acaucaucca uuaucuucac aacguggaug gauacgacgg cauugaacug     1740

aaggggaucg agaaacaauu uaaugccucu cucuccaccu aucaugaccu gcugaagauu     1800

aucaaagaua aggaguucau ggacgauccg aagaacgaag aaauucuuga gaauaucgug     1860

cacacacuca ccaucuuuga ggacagagaa augauuaaac agcgccuggc gcaguacgau     1920

agcaucuucg acgagaaggu cauuaaagcu cugacgcgga gacauuauac aggauggggc     1980

aagcucucug ccaaacugau caacggcauu ugcgauaagc agaccgggga caccauccuu     2040

gauuaccuga ucgacgaugg aaagauuaau cggaacuuua ugcaacucau caacgacgau     2100

ggccuguccu ucaaagaaau uauccagaag gcccaggugg uugggaaaac agacgacgug     2160

aagcaggucg ugcaagagcu gccuggaagc cccgcuauua aaaagggcau ccuucagucc     2220

aucaaaauug uugaugaacu cgugaagguc augggguaug ccccugaguc uaucgugauu     2280

gagauggcuc gcgaaaauca gaccacagcc agaggcaaga aaaacagcca gcaacgguac     2340

aagagaaucg aggacucccu gaaaaaucug gcgcccggac ucgauucuaa cauucugaag     2400

gaaaauccca cggacaacau ccagcuucag aaugaucgcc uguuccucua cuaucugcaa     2460

aacggcaaag acauguacac cgggaagccu cuggauauug accagcuuag cuccuaugau     2520

aucgaccaca ucauuccgca ggccuuuauc aaggaugaca gcauugacaa ucggguccuc     2580

acaucuucca aagauaacag aggaaagagc gacaaugugc ccucucugga gguugugcag     2640

aaacgcaagg cuuucuggca acagcugcuc gauuccaaac ugaucagcga gagaaaguuu     2700

aacaaucuua ccaaggccga acggggcggg cuggacgagc gcgauaaagu cggauucauu     2760

agacggcagc ucguggaaac acgccagauc accaagcacg uugcucaaau ccuggacgcc     2820

ucuuuuaaca cggaggugaa ugagaaaaac cagaagauua gaaccgucaa aaucauuaca     2880

cuuaagagca accugguguc caauuuccgg aaggaauuug agcuguacaa agugcgcgaa     2940

aucaacgauu aucaucacgc gcaugacgcc uaccucaaug cuguugucgc caaggcuauu     3000

cugaaaaagu auccuaaacu cgagcccgag uucguguacg gcgauuacca gaaguaugac     3060

cugaagagau acaucagccg guuuaaaccu ucuaaggaaa ucgaaaaagc caccgagaag     3120

uauuucuuuu acuccaaccu ucugaauuuc uucaaagagg aagugcacua ugccgauggg     3180

auuaucguca agcgcgagaa cauugaauac agcaaagaca caggcgagau cgcuuggaau     3240

aaggaaaagg auuuugcgac cauuaaaaag gugcucuccu auccccaggu uaacaucgug     3300

aaaaagacgg agauucaaac acacggacug gacagaggca aaccgaaggg gcuguucaau     3360

ucuaacccua gccccaagcc uuccgaggac ucuaaagaaa aucuuguccc caucaagcag     3420

ggacuggauc cucggaaaua cggcggguac gccggaauca gcaacuccua ugcugugcuc     3480

guuaaggcca uuguggagaa aggcgccaag aagcagcaga aaaccauccu ggaauuucaa     3540

gggauuucua uccucgacaa gauuaauuuc gagaacaaua aagagaacua ccugcuuaag     3600

aaaagauaua ucgaaauccu gagcacaauu acccucccca aguacucccu guuugaguuc     3660

ccggauggca cccgccggag acuggcuagc auccuuucua caaacaauaa gcgcggagaa     3720

auucauaaag gcaacgagcu gguccucccu gggaaguaua cgacccugcu cuaccacgcc     3780

aaaaauauca acaagaaacu ggaacccgag caucuugagu auguggaaaa gcaccggaau     3840

gacuuugcga aacugcugga guguguucuc aacuucaaug auaaguacgu gggagcucug     3900

aagaacggcg aacgcauuag acaggccuuu acagacuggg agaccgucga uaucgaaaaa     3960

cuuugcuucu ccuuuaucgg gcccgagaau agcaagaacg ccggacuguu cgagcucaca     4020

ucucagggcu ccgcuagcga cuuugaguuc cuggggguga aaauuccucg guacagagau     4080

uaugccccgu cuucccuccu gaaggcgacc cuuauccauc aaagcauuac gggccuguac     4140

gaaacacgca ucgaccuguc caaacucgga gaagauuga                            4179


<210>  4414
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4414
augaacaaua acaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucaucacag augacuauaa gguucccucu aaaaagauga aagugcucgg caauacggau      120

aagcacuuca uuaaaaagaa ccugaucggg gcccuucugu uugacgaggg agcuaccgcg      180

gaagauagac gguucaagcg cacagcuaga cggcgcuaca ccagacggaa aaaucgccuc      240

agauaucugc aggagauuuu uagcgaagag auguccaagg uggacucuag cuucuuucau      300

cggcuugaug acuccuuccu ggucccugaa gauaaacgcg gcucuaagua cccgaucuuu      360

gccacacucg ccgaggagaa agaauaucac aagaaguucc ccaccaucua ccaucugaga      420

aaacaccugg cugacagcaa ggagaaaaca gaucuccggc ugauuuaucu ugcccuggcu      480

cauaugauca aguaccgcgg gcacuuucuc uacgaagagu ccuucgacau uaaaaacaau      540

gauauccaaa agauuuuuag cgaguucauc ucuauuuaug acaacaccuu ugaaggaucc      600

agccugucug gccagaaugc ccagguggag gcgaucuuca cggacaaaau cuccaagagc      660

gccaagagag aacggauucu uaaacuguuc gcuuacgaga agucuaccga ucucuuuucc      720

gaauuucuga aacugaucgu uggcaaccag gccgacuuua agaaacacuu cgaucucgag      780

gagaaggcuc cucugcaauu uagcaaggac acauaugaug aagaccuuga gaaucugcuc      840

gggcagauug gagaugacuu cgccgaucug uuucuugugg cgaaaaagcu guacgacgcc      900

auccuccugu ccggcauucu gaccgucaca gauucuagca ccaaagcucc ccucuccgcc      960

agcaugaucg aacgcuauga gaaccaucag aaggaccugg cugcccuuaa acaguucauc     1020

caaaauaacc ugcaggaaaa guacgacgag guguuuucug aucaguccaa ggacggguau     1080

gccagauaca uuaacggaaa aacgacacag gaggcuuucu acaaguauau caaaaaucuc     1140

cugagcaagu uugaaggcuc ugauuacuuc cuugacaaaa uugagcggga agauuuucug     1200

agaaagcaac gcaccuucga caacgggagc aucccucacc agauucaucu ccaggagaug     1260

aaugcgauca uccggagaca aggagaacac uaucccuucc ugaaggagua caaagagaag     1320

auugaaacaa uccugaccuu ucgcauuccu uauuacguug gcccgcucgc ccggggcaac     1380

agaaauuucg cuuggcugac ccgcaacucc gaucaggcca uccggcccug gaauuuugag     1440

gaaauugugg accaggccuc uagcgcugag gaguucauca acaaaaugac aaauuaugau     1500

cuuuaccugc cugaagagaa gguccucccc aaacacuccc ugcuguacga aacguuugcc     1560

guguauaacg agcuuaccaa ggugaaauuc auuagcgaag ggcucagaga cuaccaguuu     1620

cuggauucug gacaaaagaa gcagaucguc aaucagcugu ucaaagagaa gcgcaaaguu     1680

acagagaagg acaucauuca guaucuccau aacguggaug gcuacgacgg gaucgaacug     1740

aaaggaauug agaagcaauu uaaugcgucc cuuagcaccu aucacgaccu gcucaagauc     1800

auuaaagaua aggaguucau ggacgauccu aaaaacgaag aaauccugga gaauaucgug     1860

cauacacuga ccauuuuuga ggaccgggaa augaucaagc agagacuugc ucaguacgcc     1920

ucuauuuucg auaaaaaggu caucaaggcc cucacgcgcc ggcacuauac aggcuggggg     1980

aaacuguccg cuaagcugau uaacggaauc ugcgacaaaa agaccggcaa aaccauccuc     2040

gauuaccuga uugacgaugg cuacagcaac agaaauuuua ugcagcuuau caacgacgau     2100

gggcuguccu ucaaggacau uauccaaaag gcccaggugg uuggaaaaac aaaugaugug     2160

aagcaggucg ugcaggagcu ccccggcucu ccggcuauua aaaaggggau ccugcaaagc     2220

aucaaacugg uugacgaacu ugugaagguc augggacaug ccccugaguc cauugugauc     2280

gaaauugcgc gggagaacca gaccacagcc cgcggcaaaa agaacucuca gcaaagauau     2340

aagcggaucg aggacgcucu caaaaaucug gcccccgggc uggauagcaa uauucucaag     2400

gaacacccua cggacaacau ccagcugcag aaugauagac uuuuccugua cuaucuccag     2460

aacggcaaag acauguacac cggagaggcu cuggauauua aucaacuguc cagcuaugac     2520

aucgaucaca ucguccccca ggccuuuauu aaggacgauu cucuugacaa ccgcgugcuc     2580

acauccagca aagauaaucg gggcaagucu gacaacguuc ccucccugga agugguccag     2640

aagagaaaag cguucuggca gcaacugcuc gacagcaagc ugaucucuga gcauaaauuu     2700

aauaaccuua ccaaggccga gcgcggggga cuggaugaaa gagacaaagu gggcuucauu     2760

cggcgccagc ucguugagac aagacagauc accaagcacg uggcucagau ucuggaugcc     2820

cgguuuaaca cggaagucaa ugagaaggac aaaaagaacc gcaccgugaa aaucaucaca     2880

cuuaagagca aucugguguc caacuucaga aaagaauuua agcuguacaa gguucgggag     2940

auuaaugauu aucaucacgc ucacgacgcc uaccucaacg ccgucguggc uaaagcgauc     3000

cugaagaaau acccuaagcu cgagccgggg uucguguaug gagauuacca aaaauaugac     3060

auuaagcgcu acaucagcag aucuaaggau cccaaagaag ucgagaaggc caccgaaaaa     3120

uauuucuucu acuccaaucu gcuuaacuuu uucaaggagg aagugcauua ugcugacggc     3180

acaauuguua aacgggagaa uaucgaguac agcaaggaua ccggggaaau cgccuggaac     3240

aaagagaagg acuucgccac gauuaagaaa gugcuguccc ucccucaggu caauaucgug     3300

aagaaaacag aaauucagac ccacggccug gaucgcggaa agcccagagg ccuguuuaac     3360

ucuaauccua gccccaaacc guccgaggac cggaaggaaa accuuguucc uaucaagcaa     3420

gggcuggacc ccagaaaaua cggaggcuau gcugggauuu cuaauagcua cgccgugcuc     3480

gucaaggcga ucauugagaa aggagcuaag aaacagcaga agacagugcu ggaguuccag     3540

ggcaucucca uccucgauaa gauuaacuuu gagaaaaaca aggaaaauua ucugcuugaa     3600

aaaggguaca ucaagauucu gucuaccauc acccucccua aauauagccu guucgaguuu     3660

cccgacggca cacgccggag acuggccucc auucuuagca cgaacaauaa gcgcggagag     3720

auccauaagg gcaacgaacu gguuaucccu gagaaauaca ccacacuccu guaucacgcc     3780

aagaauauua acaaaacccu cgaacccgag caucuggaau acguggagaa gcaccggaau     3840

gauuucgcua aacuucugga guacguccug aacuuuaaug acaaguaugu gggggcccuc     3900

aaaaacggag aaagaauccg ccaagcguuc auugauuggg agacagugga caucgaaaag     3960

cuguguuuuu cuuucauugg cccgagaaau uccaagaacg cugggcuuuu ugagcugacc     4020

agccagggau cugccuccga uuucgaauuu cucggcguca aaaucccucg guaccgcgac     4080

uauacgccca gcucucugcu caaugccaca cugauccacc aguccauuac cgggcuuuac     4140

gagacccgga ucgaucugag caagcugggc gaggacuga                            4179


<210>  4415
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4415
augaagaacu accacaucgg ccuggacauu gggaccagcu ccgugggauu cgccgcuaga       60

gaugacgaga auaaccucau ccgggucaaa ggcaagaaug uuauuggggc ccgccuguuu      120

gcggaaggaa aaacagcuca ggagagacgg auggcccgca cgucuagacg gcgcuauaac      180

agacggaagu ggcgccuuag acugcucaau gauaucuucc augacgaaau ugugcacaaa      240

gauaaggacu uuuucaaccg gcugaagcaa agcuccaucu cucccaaaga ugaccgcaag      300

aaauacuuug gcagccuucu guucccugag cucuccgauu cugaauuuua ucauaauggg      360

aaccagacca ucuaccaccu gagacaucgg cugaugacaa gcaaugagaa ggccgacauu      420

cgcgagaucu aucucgcucu gagacacauu gugaaauacc ggggaaacuu ccuugaugac      480

accccggucu ccagcuuuga agccucugau cugaagcucg gcuccuucau ccccacacug      540

aaugaccuuu uugagaccau ugacgugaac uauguuuuca auagcgaaaa cacaaaucag      600

aucgagucua uucugcucaa caacgaucug agaaauuccg acaagagcaa acagcuggug      660

gaucuccugu accgcaacgu cucugacgug gaugacaagu cccuugagaa acaauuuaau      720

aaguuccgga aagauaucag caaggaaauc gcuaaauccu ucguuggcuc uaaguuuaac      780

gugaauaagc ugcucgccua cgagaccgac gaugggagca aaauuucccu gagcuucucu      840

gacgcgucca gcgaugacaa gaugcuugaa cuggccgaca accucgauga cgagagaauc      900

aauauucuga acgcucugaa agucaucuau ucucgcgugc ggcucaauca gauuaucccu      960

aacggaaugg gccugagcga auccaugauc gccucuuacg agcuucacaa gaaacagcug     1020

aaugaucuca agaagcugcu uaacgugcug cccuuucaug agaaauaugc ugucaagguu     1080

gccuacgcgg ccuauauugg gaauauggac gcucagaaau ucacgcaagc cgaacucagc     1140

accaucgagu cccugaagga aaaagauagc auuaagaagg ugaucgacug caaaacagga     1200

cacauuacca acgagucucu gaucuccgug aucaaucaga uugucgugaa cccuuaccuc     1260

gaaaacagcg auuuugagcu gaucgacauu guuaagaaaa ucgaucagga cauuaagaau     1320

ggcgaguauc uucugaaaca gagaaacaau caaaacggga auauccccca ucagcuccac     1380

cagauggaaa uggaucaaau uaucgagaag cagucuaagu acuacccuuu ccugggagaa     1440

cugaacccga augagaaccg ccggcacauc gcuaaauaua agauuuccga acuuguggcc     1500

uuuagagucc ccuacuaugu gggcccucuc aucacacccg aggagcaggc caaaaccagc     1560

ggcgcuuccu ucgcguggau gaagcgcaaa gaagacgggg agauuacgcc uuggaauuuu     1620

gaucagaagg uugaccggga agccacagcu ucuagauuca ucaaacgccu gaccgugaag     1680

gacacauacc uguuuaacga ggauguccuc cccgacaaua gccugcuuua ucaacaguuc     1740

aaggugcuga acgagcucaa ugucgugaaa guuaacaaua agcggcuguc ccaggccgaa     1800

aaacagggag ccuuuaacga ucuguucaag gugaccaaaa ccgucacagc uaagagacuu     1860

aagaacuacc ucgugucuaa uuucaaccau cuggacacga uugagaucac cggccugagc     1920

gauggggaaa aauuuaauaa cggacucaca uccuauguug acuuccaaaa gaucuuuggc     1980

gauaaaauua auaaccugga gcuuauggac gauuucgaaa gccugaucga guggucuacc     2040

guguuugagg accggcacau uuucgaagag aagguccgca augaaaucaa cuggauuucc     2100

gagagcgaac ucaaacagcu gguggccaag agauacucug gguggggacg gcuguccagc     2160

aaacuucucg ugggcaucaa uaacgaugag ggcagaaaua ucauugagca gcuguuugaa     2220

acaucugaga ccuucaugga agcgaucagc cgcccggagg uuaaaaagca gauugacgaa     2280

aucaacuccg auuuuauuaa aaauacggac aucgagagcg uccuggcuga cgccuacaca     2340

ucuccucaaa acaagaaagc cauccgggag accuauaaga uugugaagga uauccagaaa     2400

gcuaugggag ggaaggcccc cgaauccauu agcaucgagu ucaccagaaa uccugaagac     2460

aaaggcgagc ucacacgcuc cagacauucu gagauuagcc ggaaguacaa agaacugucc     2520

aaggagaucu cuaaagaacu uaacgaugag cucaagagca agaaaaacuc cauggacauu     2580

gauaaguauu accuguauuu uaugcagaug gggaaagacc uguacgaugg aaagucuauc     2640

gacaucgaug accugaauaa cuaucagauu gaucacaucg ugccccagag cuucuacaaa     2700

gacgacuccu uugauaaucg cguccuuacc uacuauacaa acaaucaaaa gaagggcaac     2760

aauacgcccc ucaacgggcu gaauauuagc aacgaaacca gaaaacugug gucucaucuc     2820

cacgaccugg gccuuaucuc caagcggaaa uaccugaauc ucacaaugca gggacuggau     2880

agccugucua aguauaccaa acacggcuuc auucgcagac aacuugugga gacaucccag     2940

guuaucaagc ugguggcuaa cauccucagc gacgaguucu cugccgauaa uaccaagauu     3000

aucgaaguca aagcgaagau gaacucccag augcggcaua gcuuuaaucu guacaaaauu     3060

cgcgagcuca acgacuauca ccaugcccug gaugcuuacc uuacgaccuu caucggggga     3120

uaucuguaca auagauaccc uaagcuccgg ggcauguuug uguaugggga cuucaaaaag     3180

uuugauguua acgacgccaa caaugugaag acacugaacu uccugcacga uauuaccaau     3240

ccguccgaca aauuugucaa ccagaucuac gaugaacaaa gcggagagcu uauccuggac     3300

cgcaagcacg cuauugaaac aaucaaaaag auuuaucagu acaaauucau gcucaucacc     3360

agagaggugu cuacgaagga cgauaagcug agcgaccaga caguuuauuc cgccaaacgg     3420

auuaauaacg uggcgucucc caucccuguc aagaaagaua gcaaucccga gcucuacggc     3480

uuuagaaccg ggaacgugga ccaucacaug gugauuguca aguauccuaa aaaggauucc     3540

uucguuuaca aagugguggg cauccccauc cgcuuuaugc aggaacugcg gucuagcaag     3600

aaucaauccg aguacgaaaa cacacuuaau cagauugucc aggccaagcu gaacagaaag     3660

aaguucgacu uccgcguggu uguggauaau gucauguaug gacagcucau ccaagacggc     3720

gggcagaaau uuacccuggg aucuagcacc uaccgguaua acgcuaagca gcuggugcuu     3780

gauauggacu cccugcgcau ucucagcgau gagaaauaca gaaauucucu gacagacgau     3840

gaaaagaacu ccgaguucau caacauuuau caacagguuc ucaauguggu caacgagaag     3900

uuugacgugu ucgacguucg gggcacgaga gauaaacuga ccaaggggcu ugacaaauuu     3960

cugaauauca caagcaacga uaaggaaauc gugcugcuca aaauucuguc uggacuucau     4020

uguaaugccg cucgcgucaa gaucaacgag auuggcauca auaccuccuu cggggaacug     4080

cagacaaccc acggcauugu gcucagcgag aacuccuaca ucaucuauca gucuccgagc     4140

ggacuguuug agcggaaggu gagacucaaa gaccuguga                            4179


<210>  4416
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4416
auggccaaca agaccuggua ccugggccuc gacaucggga gcgcuuccgu gggaugggcc       60

gcgacagaua cgggcuauaa aauuaucaga aagaauaaaa agcggcugug gggaguccgc      120

cuuuucgagg aagcuaaaac cgccgaggac agacgggggu accgcucuag cagacggcgc      180

cuggccagac ggaaguggcg ccucaaccug cuugaagaac uguuuacauc cgaaguuaag      240

aaaguggaug agaauuucuu ucucagacug aaggagucuc aguaucacua cgaagacaaa      300

acccauaagg ugcccuaugc uauuuucaac gauaaagacu acacagauaa ggacuauuac      360

aaggauuacc cuaccaucua ucaucugcgg agcaaacuca ugacagagga aaauccggac      420

auucgcaagg ucuuucuggc cauccaccac aucauuaaaa acagaggcca uuuccuucug      480

caagggcagu ccuuuaagga uggaaaucuc aacaaucuga ucaaagagcu ucuggagcuc      540

gacauucugc acgugggcuu cgaaguuacc gaggaagugg ucgauaagau cgcugacauu      600

ucucuggaga aaaagacgag caaggacaaa cucaacgaua ucaaggaacu guaccccaaa      660

gagaagcagc uucuggaggu guuucggcuc auuuucggcg ggaaaaccuc ccuggacaaa      720

cuuuuugcca ucgaugaaua uaaggagcug gacgcggcca ucaaaagcgu uucuuucaag      780

gaaaagauuu acgaggaagu gcgccacgau uaugagcagg uccucuccaa uuacaucgag      840

cugcuggacc ucgcuaagcu gguguaugau agcauuaucc uuucugacau uaaaaaggaa      900

ggaaagacac uguccgagag caaaguggaa cucuuugaga agcauagaga ugaccugcuu      960

aaacugaaga accucgucaa aaaugauucu aagcuguccg aagacaagaa aguugagcug     1020

uacgcccuca ucuucaagga ggacaaagau aagggcacca acuacgugaa uuauauccgg     1080

aaaagcgaag aggggaaggg augcaacuac gaagacuuca aaaaguuucu ggugaaggag     1140

cuugcuaaac uggaggaauc cucugucaag acagagauua ucaaagaucu cgaacuggag     1200

caauuccugc cucuucagag aaccaaggac aacagcgugg uucccuauca gauucacaaa     1260

gaagaacucg ugaagauccu ggauaaugcc gcgcgcuacc auuccuuucu gaacgagaag     1320

gacgaaagcg gcuauaaugu ccgggagaaa gugauucagc uccuggaguu cagaaucccu     1380

uacuauguug ggccccuuaa cucuuccaag aaagccaagg agggaggcuu ugcuuggagc     1440

gugcgcaaua cgggcuacga aaacacaccu gucaccccgu ggaauuacuc uaaagugauc     1500

gaugagagcg ccuccgcuga gaaguucauu acaaaccuga ccaauaagug uaccuaucuc     1560

aaaggggaag acguccugcc caagucuagc cugcuuuacu ccgaguuugc ccuccugaac     1620

gaacugaaug cccucaaaua ugauggaaac cggaucagcc uggaggcuag aaauacaauu     1680

aucgaaaaac uuuucaaaca acagggcaag aaggugacga aaaccucuau uaagaaccug     1740

cucaaagcgg agggguacau cgacggaaag ggcgagauua cagggaucga uaucaccguu     1800

aaaaaugacc ugaaguccua ucgcgauuuu aaaaagauuc ugggaaacaa guucaacagc     1860

gaacacgugg agaauaucau ucuuuggauc acacucuacg gcgaaucucg gaaacugauu     1920

aaggccaaaa ucgaguccgu cuauggcgag guguacagca aggacgaaau caccaaaaug     1980

uccagacugg uuuacaagga cugggggcgc uuuucucgga agcuccugac ggagcuugug     2040

agcaaaaagc uguauaacga agagacagga gaaugccuca auauuaucgg cgcuaugaga     2100

cagaacaaua uucuguucau ggagcugcuu gccgaucgcu uugacuacuc caaacagauc     2160

gucgaguuca acaaggaacu ccaagaggau gugaccgaaa uugccccuga gauccuggac     2220

gaucuguaug ugucucccgc uguuaaacgg agcaucuggc agaccgucag aauuguggag     2280

gagcucaaga agaucauugg gugugccccu gcgaaaaucu ucguggagac aacccggucc     2340

aaucaggaaa agaaaaagcc cacagacagc cgcaaaaagc aacuggagcu ugcuuacaag     2400

gccgucaaaa aggaugugaa agaacuggag aaggaaauug gaacgaucaa cuuugacguu     2460

cucaaugaua gacugucuuc cguggagccg agcaaacuga aggccaagaa acuuuaucug     2520

uacuauaccc agcucggccg gugcauguac ucugaggaac cuaucaacau uuccgagcug     2580

uucgacaaua acaaguacga uaucgaccac auuuaucccc agagcaaaau caaggaugac     2640

ucuuuuacaa auaccguccu cgugaaaaga gaaagcaacg cugccaagac agaccgcuac     2700

ccucuggggu ccgauauuca gacccccagc aaucggagau ucuggaaauu ucuuaaggag     2760

aagggccuga ucucugacga gaaauauaac cgccucguua gaacggaaga guucuccgau     2820

gaggaacuga gcggauuuau ugcucggcaa cugguggaaa ccucccaguc ugucaaggcc     2880

guggcgagca uccuuggcga gcugaacccc gagacaucca ucuguuacuc uaaagccgaa     2940

aauguuagcu ccuuccgcca gaacuuuggg aagauuaaaa agggagacag agauucuaag     3000

aauaacgacc agcucaucaa agugcgggag auuaaugauu aucaucacgc uaaggacgcc     3060

uaccugaacg ucgugguggg caaugucuau gauaccaaau ucacacgcaa cguuuuuaau     3120

uucaucaagg acaaaaagga ugggagaaag uacagccuca acaaaguguu uuacgaaaau     3180

guguccgaca gcaagaccgu cgcuugggag auggacaaaa cgauucauca aguggaaaag     3240

guuaugaaca auaacaauau cauggugaca cggcgcaccu cugagcagaa aggaggccug     3300

uucgaugcca cagucuauaa ggcgaaggug gccgcuaaag ccaaggacgg gguuuacuau     3360

ccucuuaaaa ccuccaacag cguggucaag gauguggcua aauacggcgg auauaccucu     3420

aucggcauug ccuacuauuc caucuucgag uacacaauug uuaacaagaa aggggaagag     3480

aagaucacga gaauuauccc gauccccauu uacaucagcc agaauauuaa ggacucuacc     3540

gcccugaucg aauuuggaaa aucccagauu uucugccggg cuuaugagaa gaucaaagau     3600

uuuaagcucc uguaccgcaa acuguguauu ggcagcaaga ucaacaucaa uaacuaucaa     3660

uacuauauug ggggaaaguc cgacgaaaga uucuacuaug auagcgcgau ccaggugaca     3720

cuugacaaaa agucugagaa auaccugaag auucucagca aauaccagaa uuggaagaag     3780

gagaacaaag auggcgaacu gugggagaau aucaccaagg aaaacaauau ugagcucuau     3840

aacaaacugg ucgagaagau gaacuccgaa aucuuuacaa agaagaaguc uaauaaauac     3900

aaugagcuua acagcgaaga gauccggaau aacuucauua agaucuccgu ggaggaacag     3960

gccaaagugc ugcuggagau ucucaaucug cuuaccaaca agaaaucuau cuuugaccuc     4020

aagagcauug auaucaaagc ugccagaggg aagaucuccu ucaaccugac gucucugauu     4080

caauuuagca ucauugaaca guccaucaca ggcuucuaug agaaggaaau uaccaucauu     4140

ggagacaaag gcaaugauau ggagaacaau aacagcuga                            4179


<210>  4417
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4417
augaacaaua acaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucaucacag augacuauaa gguucccucu aaaaagauga aagugcucgg caauacggau      120

aagcacuuca uuaaaaagaa ccugaucggg gcgcuucugu uugacgaggg aaccacagcc      180

gaagauagac ggcucaagcg caccgcgaga cggcgcuaca caagacggaa aaaucgccug      240

agauaucuuc aggagauuuu cagcccugaa aucuccaagg uggacucuag cuuuuuccau      300

cggcuggaug acuccuuucu cgucccggag gauaaacgcg gcucuaagua ccccaucuuc      360

gcuacccugg ccgaagagaa agaguaucac aagaacuuuc cuacaauuua ccaucugaga      420

aagcaacucg ccgacagcaa agaaaaggcu gaucugcggc uuaucuaucu ggcccucgcu      480

cacaugauua aauaccgcgg gcauuuccug uacgaggaau ccuuugacau caagaauaac      540

gauauucaga aaaucuucaa ugaguuuauu agcaucuaug acaacaccuu cgagggaucu      600

ucccuuagcg gccagaaugc ccagguggaa gcgaucuuua cggacaagau uucuaaaucc      660

gccaagagag agcggguucu gaaacucuuc cccgaugaaa agagcaccgg ccuguucucu      720

gaguuucuga agcucaucgu ggggaaccaa gcugaguuca aaaagcacuu ugaccuggaa      780

gagaaagccc cucuucaguu cuccaaggau acauacgacg augaccugga aaaucuccug      840

ggacagauug gcgauggguu ugcugagcuu uucgucgccg cgaagaaacu guaugacgcc      900

auccuccuga gcggcauucu gaccgugaca gaucccucca ccaaggcucc ucucucugcc      960

agcaugaucg aacgcuacga gaaccaccag aaagaccugg cugcccuuaa gcaauuuauc     1020

cagaacaauc ugcaggaaaa auaugaugag guuuucuccg accagagcaa ggacggauac     1080

gccggguaua uuaacggaaa gacgacacaa gaggcuuuuu acaaauacau caagaaucuc     1140

cugucuaaau ucgaaggcuc cgauuauuuu cuugacaaga uugagagaga agauuuccug     1200

aaaaagcagc ggaccuuuga caacggcagc aucccgcauc agauucaccu ccaagagaug     1260

aaugcgauca ucagacgcca gggggaacau uaccccuucc ugcaggagaa caaggagaaa     1320

auuaagaaaa uccugacauu ccggauuccu uauuacgugg gaccccucgc cagaggcaau     1380

ggggauuuug cuuggcugac ccgcaacucu gaccaggcca uccggccuug gaauuucgaa     1440

gagauugucg aucaagccag cuccgcugaa gacuuuauca acaagaugac caauuaugau     1500

cuuuaccugc ccgaggagaa agugcucccg aagcacucuc ugcuguauga aacauucgcc     1560

guguacaacg agcuuacgaa agucaaguuu auugcggaag gacucagaga cuaccaguuc     1620

cuggauagcg gccagaagaa acagaucguu aaucaacugu uuaaggagaa acgcaaggug     1680

accgaaaaag acaucacaca guaucuccac aacguggaug gguacgacgg aauugagcug     1740

aagggcaucg agaagcaguu caaugcuucc cuuagcaccu aucaugaccu gcucaaaauu     1800

aucaaggaua aagccuuuau ggacgaugcc gaaaacgagg cuacacugga aaacauuauc     1860

cacacccuga cgaucuucga ggaccgggaa augauuaagc agagacuugc ccaauacgau     1920

ucucucuuug acgagaaagu caucaaggcu cugauucgcc ggcauuauac aggcuggggg     1980

aagcuguccg ccaaacucau caauggaauu ugcgauaaga aaaccggcaa gaccauccug     2040

gacuaccuua ucgaugacgg guauagcaac agaaauuuca ugcagcugau uaacgaugac     2100

ggacucucuu uuaaagauau cauucagaag gcucaggugg uuggccgcac aaaugacgug     2160

aagcaaaucg uccacgagcu gccugggucc cccgccauua aaaagggcau ccugcagagc     2220

aucaaaauug uggacgaacu uguuaagauc augggacaua ccccugaguc cauugugauc     2280

gaaauggcuc gggagaacca gacaacggcc agaggcaaaa agaacucuca acagcgguac     2340

aaacgcauug aagaugcucu caagaaucug gcccccgggc uggacagcaa uauccucaag     2400

gaguacccca ccgauaacau ucagcugcag aaugacagac uuuuccugua uuaccuccaa     2460

aacggaaaag auauguauac aggcgagccu cuggacauca aucagcuguc cucuuacgau     2520

aucgaccaca uugucccgca ggcguucauc aaggaugaca gccuugauaa ccgggugcuc     2580

accuccagca aagacaauag agggaagucu gacaacgucc ccucccugga agugguucag     2640

aaacgcaagg ccuuuuggca acagcugcuc gauagcaagc ugauuucuga gcggaaauuc     2700

aacaaucuua caaaggcuga aagaggaggc cuggacgagc gcgauaaagu gggguuuauc     2760

agacggcagc ucgucgagac ccgccagauu acgaagcacg uggcccaaau ccuggacgcu     2820

agauucaaca ccgaaguuac agagaaagau aagaagaauc ggaacgugaa aaucauuacc     2880

cugaagucca aucuugucag caacuuucgc aaagaguuca agcuguauaa agugagagaa     2940

aucaaugacu accaucacgc ccaugaugcc uaucucaacg cugugguugc gaaggccauu     3000

cugaagaaau acccuaagcu cgaacccgag uuugucuacg gcgacuauca gaaauacgau     3060

cugaagcggu auaucucucg cagcaaagac ccuaaggaug uggagaaggc uacagaaaaa     3120

uacuucuuuu auuccaaucu ucugaacuuc uuuaaggagg aagugcacua cgccgacgga     3180

accauuguca aaagagagaa uaucgaauau agcaaggaua cgggcgagau cgccuggaac     3240

aaagagaagg acuucgcuac aauuaaaaag gugcucucuc ugccccaggu uaauaucgug     3300

aagaaaaccg aaauucaaac acacgggcug gaccggggaa agccgcgcgg ccuuuucaac     3360

uccaauccua gccccaaacc uuccgaggau ucuaaggaaa accugguccc caucaaacag     3420

gggcucgacc cuagaaagua cggaggcuac gccgggauua gcaacuccua ugcggugcug     3480

guuaaggcua ucauugagaa aggcgccaag aaacagcaga agaccgugcu cgaauuucaa     3540

ggaaucucua uccuggauaa aauuaauuuc gagaagaaca aggagaauua ccuucuggaa     3600

aaaggcuaua ucaagauucu cagcaccauc acacugccca aauacucccu guuugaguuc     3660

ccggacggga cgcggagacg ccuugccucu auucugagca ccaacaauaa gcggggagaa     3720

auccauaaag gcaacgagcu cgucaucucc gagaaguaua caacccugcu cuaccacgcu     3780

aagaauauua acaaaacacu ggaaccugag caucuugaau auguggagaa gcacagaaau     3840

gauuuugcca aacugcugga aagcguucuc gacuucaacg auaaguacgu gggggcgcug     3900

aaaaauggag agcgcauccg gcaggcuuuu auugacuggg agaccgucga uaucgaaaaa     3960

cuuuguuucu cuuuuauugg ccccagaaac uccaagaaug ccgggcuguu cgagcucacg     4020

agccagggcu cugccuccga cuuugaguuc cugggaguga agaucccccg cuaccgggau     4080

uauacaccua gcucucuccu gaacgcuacc cuuauccauc aguccauuac cggccuguac     4140

gaaacaagaa ucgaccugag caagcucggg gaagauuga                            4179


<210>  4418
<211>  4179
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4418
augaacaaua acaauuacag caucggccug gacauuggga ccaacuccgu gggaugggcc       60

gucaucacag augacuauaa gguucccucu aaaaagauga aagugcucgg caauacggau      120

aagcacuuca uuaaaaagaa ccugaucggg gcucugcugu uugacgaggg agccaccgcg      180

gaagauagac gguucaagcg cacagcuaga cggcgcuaca ccagacggaa aaaucgccuc      240

agauaucugc aggagauuuu uagcgaagag auguccaagg uggacucuag cuucuuucau      300

cggcuugaug acuccuuccu ggucccugaa gauaaacgcg gcucuaagua cccgaucuuu      360

gccacacucg ccgaggagaa agaauaucac aagaaguucc ccaccaucua ccaucugaga      420

aaacaccugg cugacagcaa ggagaaaaca gaucuccggc ugauuuaucu ugcccuggcu      480

cauaugauca aguaccgcgg gcacuuucuc uacgaagagu ccuucgacau uaaaaacaau      540

gauauccaaa agauuuuuag cgaguucauc ucuauuuaug acaacaccuu ugaaggaucc      600

agccugucug gccagaaugc ccagguggag gcgaucuuca cggacaaaau cuccaagagc      660

gccaagagag aacggauucu uaaacuguuc gcuuacgaga agucuaccga ucucuuuucc      720

gaguuccuga aacugaucgu uggcaaccag gccgacuuua agaaacacuu cgaucucgaa      780

gagaaggcuc cucugcaauu uagcaaggac acauaugaug aagaccuuga gaaucugcuc      840

gggcagauug gagaugacuu cgccgaucug uuucuugugg cgaaaaagcu guacgacgcc      900

auccuccugu ccggcauucu gaccgucaca gauucuagca ccaaagcucc ccucuccgcc      960

agcaugaucg aacgcuauga gaaccaucag aaggaccugg cugcccuuaa acaguucauc     1020

caaaauaacc ugcaggaaaa guacgacgag guguuuucug aucaguccaa ggacggguau     1080

gccagauaca uuaacggaaa aacgacacag gaggcuuucu acaaguauau caaaaaucuc     1140

cugagcaagu uugaaggcuc ugauuacuuc cuugacaaaa uugagcggga agauuuucug     1200

agaaagcaac gcaccuucga caacgggagc aucccucacc agauucaucu ccaggagaug     1260

aaugcgauca uccggagaca aggagaacac uaucccuucc ugaaggagua caaagagaag     1320

auugaaacaa uccugaccuu ucgcauuccu uauuacguug gcccgcucgc ccggggcaac     1380

agaaauuucg cuuggcugac ccgcaacucc gaucaggcca uccggcccug gaauuuugag     1440

gaaauugugg accaggccuc uagcgcugag gaguucauca acaaaaugac aaauuaugau     1500

cuuuaccugc cugaagagaa gguccucccc aaacacuccc ugcuguacga aacguuugcc     1560

guguauaacg agcuuaccaa ggugaaauuc auuagcgaag ggcucagaga cuaccaguuu     1620

cuggauucug gacaaaagaa gcagaucguc aaucagcugu ucaaagagaa gcgcaaaguu     1680

acagagaagg acaucauuca guaucuccau aacguggaug gcuacgacgg gaucgaacug     1740

aaaggaauug agaagcaauu uaaugcgucc cuuagcaccu aucacgaccu gcucaagauc     1800

auuaaagaua aggaguucau ggacgauccu aaaaacgaag aaauccugga gaauaucgug     1860

cauacacuga ccauuuuuga ggaccgggaa augaucaagc agagacuugc ucaguacgcc     1920

ucuauuuucg auaaaaaggu caucaaggcc cucacgcgcc ggcacuauac aggcuggggg     1980

aaacuguccg cuaagcugau uaacggaauc ugcgacaaaa agaccggcaa aaccauccuc     2040

gauuaccuga uugacgaugg cuacagcaac agaaauuuua ugcagcuuau caacgacgau     2100

gggcuguccu ucaaggacau uauccaaaag gcccaggugg uuggaaaaac aaaugaugug     2160

aagcaggucg ugcaggagcu ccccggcucu ccggcuauua aaaaggggau ccugcaaagc     2220

aucaaacugg uugacgaacu ugugaagguc augggacaug ccccugaguc cauugugauc     2280

gaaauugcgc gggagaacca gaccacagcc cgcggcaaaa agaacucuca gcaaagauau     2340

aagcggaucg aggacgcucu caaaaaucug gcccccgggc uggauagcaa uauucucaag     2400

gaacacccua cggacaacau ccagcugcag aaugauagac uuuuccugua cuaucuccag     2460

aacggcaaag acauguacac cggagaggcu cuggauauua aucaacuguc cagcuaugac     2520

aucgaucaca ucguccccca ggccuuuauu aaggacgauu cucuugacaa ccgcgugcuc     2580

acauccagca aagauaaucg gggcaagucu gacaacguuc ccucccugga agugguccag     2640

aagagaaaag cguucuggca gcaacugcuc gacagcaagc ugaucucuga gcauaaauuu     2700

aauaaccuua ccaaggccga gcgcggggga cuggaugaaa gagacaaagu gggcuucauu     2760

cggcgccagc ucguugagac aagacagauc accaagcacg uggcucagau ucuggaugcc     2820

cgguuuaaca cggaagucaa ugagaaggac aaaaagaacc gcaccgugaa aaucaucaca     2880

cuuaagagca aucuuguguc caacuucaga aaagaauuua agcuguacaa gguucgggag     2940

auuaaugauu aucaucacgc ucacgacgcc uaccucaacg ccgucguggc uaaagcgauc     3000

cugaagaaau acccuaagcu cgagccggag uucguguaug gggauuacca aaaauaugac     3060

auuaagcgcu acaucagcag aucuaaggau cccaaagaag ucgaaaaggc caccgagaaa     3120

uauuucuucu acuccaaucu gcuuaacuuu uucaaggaag aggugcauua ugcugacgga     3180

acaauuguua aacgggagaa uaucgaauac agcaaggaua ccggcgagau cgccuggaac     3240

aaagaaaagg acuucgccac gauuaagaaa gugcuguccc ucccucaggu caauaucgug     3300

aagaaaacag agauucagac ccacgggcug gaucgcggca agcccagagg acuguuuaac     3360

ucuaauccua gccccaaacc guccgaagac cggaaggaga accuuguucc uaucaagcaa     3420

ggccuggacc ccagaaaaua cgggggauau gcuggcauuu cuaauagcua cgccgugcuc     3480

gucaaggcga ucauugagaa aggggcuaag aaacagcaga agacagugcu ggaauuucag     3540

ggaaucucca uccucgauaa gauuaacuuu gagaaaaaca aggaaaauua ucugcuugag     3600

aaaggcuaca ucaagauucu gucuaccauc acccucccua aauauagccu guucgaguuu     3660

cccgacggga cacgccggag acuggccucc auucuuagca cgaacaauaa gcgcggcgaa     3720

auccauaagg gaaacgagcu gguuaucccu gaaaaauaca ccacacuccu guaucacgcc     3780

aagaauauua acaaaacccu cgagcccgaa caucuggagu acguggagaa gcaccggaau     3840

gauuucgcua aacuucugga auacguccug aacuuuaaug acaaguaugu gggcgcccuc     3900

aaaaacgggg agagaauccg ccaagcguuc auugauuggg aaacagugga caucgagaag     3960

cuguguuuuu cuuucauugg accgagaaau uccaagaacg cuggccuuuu ugaacugacc     4020

agccaggggu cugccuccga uuucgaguuu cucggaguca aaaucccucg guaccgcgac     4080

uauacgccca gcucucugcu caaugccaca cugauccacc aguccauuac cggccuuuac     4140

gagacccgga ucgaucugag caagcugggg gaagacuga                            4179


<210>  4419
<211>  4182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4419
augaagagag ugaccgagga cuacauccug ggccucgaua uugggacaaa cagcugcgga       60

ugggccguca cggacaaaaa gaauaaccug cuuaaacugc ggggcaagac cgcuaucggg      120

ucccaccucu ucgaagaggg acauacagcc gcggaucgca gaggcuuucg gaccacacgc      180

agacggcuga aacgcagaaa guggcggcuu cgccugcucg aagagauuuu cgcugaaccc      240

auggccaagg uugacccugg guuuuucgug agacugcacc agucuugggu gagcccgcug      300

gauaaauccc ggaagaaaua ucaugccauc gucuuuccca ccgcuaagga ggacaaagcc      360

uucuaccaaa aguauccuac aauuuaccac cuccgcgaug cucugaugac ccaggacaga      420

caguuugauc uucgggagau cuuccuggcc auccaucaca uugugaaguu ucgcggaaau      480

uuccuccagg acacgcccgu uaaagauuuu gaagcgucua agaucgaggu gggcccuauu      540

cugagcaaaa ucaacggcgc cuucgcugaa aagauugucg aggaccaaga ucccaucgag      600

cuuaaugugg ccaacgcugc cgaaauugag gcgguuauca gagggaaaga cgccgacaag      660

acccuguaua aaauggauaa ggugaagaaa aucgcuaagc uccugacaga cuccaccgcc      720

aaagaagaga agaaugucgc uaaacagauu gccaacgccg ugaugggaua caagacacag      780

uuugaaacca uccugggcaa ggagauugau aaaucugaca aggcucagug ggaguucaaa      840

cucacggaug gggacgcgga ugacaagcug gaagcccuuc ugccugaucu cgacgagaau      900

gaucaaacaa ucauugcuga aaucaaaaag cuguucagcg ccaucacccu uuccacaauu      960

guggacgaga acaagagccu gucucagucc auggucgcca aauauaauaa gcaccaucag     1020

gacuacaaac uccugaaggc uuacaucgau acccugcagg accggagcau ugccaaaaag     1080

cuccugcuug cguaugaucu guacguuaac aauagacacg gacgccuccu ggaagcuaag     1140

accgaguuua aacaagacgg cacacuuacg aaggaggauu ucuauaaaac cgugaaggac     1200

aaccuggaug acucugaacu cgcccggaaa aucgcccaga agauugcugc cgauaauuuu     1260

augccgaaac agagaacaaa cuccaacggg gugauccccu uccaacugca ucagaucgag     1320

cuggacaaga uuaucgcuaa ucagagcaag uacuauccuu uucucgccgc ggaaaacccc     1380

gucgaggauc accgcaaaca ggccccuuac aagcuggacg aacuugugcg guucagaguu     1440

cccuauuacg ugggaccgau gauuaccccu gaugagcaag agaaaacauc uggcaagucc     1500

uuugcuugga ugguccgcaa agaagacggc cagaucaccc ccuggaauuu cgagcagaag     1560

guggaccggc aggaaagcgc caacaaguuu auuaaaagaa ugacgaucaa ggauacauac     1620

cugcucgggg aggacguucu gccugcuaau ucccugcuuu aucaacgcuu cgaggugcuc     1680

aacgaacuga auaacauucg gaucaaugga ucuagaauca gcauugaucu gaaacagcag     1740

aucuuuaacg accucuucga ggaaaagaaa accgucaccg agaagucccu gacaagcuac     1800

cuuaagcaga aucugcaccu ccccaccgug gaaauuaaag gccuggccga ucccacaaag     1860

uuuaacucuu cccuggcgag cuauuaccau cuuaaaucuc uccacgucuu cgacaaggag     1920

cuggccgauc cucaauauca gaaagacuuc gagaagauca uugaauacag cuccaucuuu     1980

gaggauaaga aaaucuauca ggacaagcug caugcugagu ucaaauggcu cacgccggag     2040

caguuuaagg ccauuucuac cuggcgccug caaggguggg gacggcuuag cuguagacug     2100

cucguggaac ugcacgauac aaauggccag aacaucaugg agcagcugug ggacucccaa     2160

aagaauuuca ugcagauugu uaccgagccc gauuuuaagg acgcuaucgc caaagaaaac     2220

cagaacguga cacaggucaa ugggguggag gaaauucuug ccgacgcuua caccagcccu     2280

gcgaacaaga aggccauccg gcaaguugug aaagucgugg cugauguggu uaaggccgcc     2340

ggaggcaaaa agcccgcuca guucgccauc gaguuuacgc gcgacccuga uaaaaauccc     2400

cagcucucuc auauuagagg caccaagcug cugaaggcgu accaggaaac agcuggggag     2460

cucgucgacc aaaaacugac cgauucccuu aaggaggcca ugacaagccg gaaacugcuc     2520

aaggacaaau auuuccugua cuuuaugcag accggaagag augccuauac gggcaagaag     2580

aucaacauug acgaagugac aaccaauuac cagaucgauc acauucugcc gcagucuuuc     2640

aucaaagacg auuccuuuga caaccgcgug cuuacagcua ccccucucaa ugccgaaaag     2700

agcgacgaug uccccuauaa acgguucgcg aacaauuacg uguccgaccu gaagaugacc     2760

guuggggaaa uguggaaaca cuggcaaaag gcuggaauca uuaacaagca uaaacugggc     2820

aaucuccugc uugauccuga cagacugaac aaguuucaga aaucuggguu caucaaucgc     2880

cagcucgugg agacaagcca aauugucaag cugguguccg uuauccugca gaacaaauau     2940

cccgaugccg agauuaucac ggugaaggcc ggcgacaauu cugcucuuag acagcggcug     3000

aaccucuaca agagccgcga ugucaacgac uaccaccaug ccauugaugc uuaucugucc     3060

aucaucugcg gaaauuuccu cuaccaggug uauccuaaau acagacccua uuuuguuuac     3120

ggcaaguaua aaaaguucag ccaagacccg gaucugcaga aagaccuuau uaagcacuuu     3180

aaaggguuca ccuuuaugug gccucugcuc cagaaggaua acucugaacg gaaggccccc     3240

gaggaaauca aagagaauaa cuccgaacgc auuguguucu acaagcaccc cgacaucuuu     3300

gacaaacuga gaaaggcgua caauuauaaa uacaugcugg ucagccggga gacaaccaca     3360

gagucuucca gcauguucaa gaugaccauu uuuccucgcg gagaaagaga ucggaagaaa     3420

acgcgcaaac uuaucccgaa aggcaacggg cuggacccca agaucuaugg aggcuacucu     3480

gggaauacag augccuauau ggugauugug aaaaucgaca aggcuaagga gagcauuuac     3540

aaagucaucg gcguuccuau gagagcccuc acccggcuga accaggcuaa gaaacaagga     3600

aauuaucagg aggaacucca ucaggugcug gaaccccaga uuaugcuuga uaagaacggc     3660

aaaccuaaga gaggggugaa gggauuccgc gucaucaaag accacgugcc cuuuaagcaa     3720

guugugcugg auggcgacaa aaaguucaug cucuacuccg cccaguacaa aaccaaugcc     3780

aagcagcugg cucugagcca ggagacaaug cggaucguca ccgauaaccu uaaaaagggg     3840

gaggaccaag auaagcugcu cgugaaagcg uaugacgaaa uucugcagaa gguugauugu     3900

uaccucucuc uguucgacac auccaauuuu agaaacggac uucaucuggg ccgcaauaaa     3960

uucaucgacc uggaggugaa cgauaagaaa auuacgcuca gcaacauccu gaaugggcuu     4020

cacgacaaca ugguccuggg caaucucaag aacauuggaa ucggcacccc ucuggggcag     4080

augcaagaaa aguccggaau uacacucucu cccgaggccg ugcugaucuu ucagagcccg     4140

accggccuuu ucgaaaaacg gguuaagauc gcugaucugu ga                        4182


<210>  4420
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4420
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggc       60

ugggccguua cagacacgga uuauaaacug cuuaaggcua acagaaaaga ccuguggggg      120

augcggugcu uugaaaccgc cgagacagcg gaagugcgca gacuccaccg gggagcucgc      180

agacggauug agcgcagaaa gaagcggauc aaacugcuuc aggaacuguu cucccaagag      240

auugccaaga ucgaugaggg cuuuuuccag cgcaugaaag aaucucccuu uuacgccgag      300

gacaagacca uucuccagga aaaugcucug uucaacgaua aagacuuuac agauaagacc      360

uaucauaagg ccuacccuac aaucaaucac cugaucaagg cuuggauuga gaacaaggug      420

aaaccggacc ccagacuccu guaucuugcc ugucauaaua ucauuaagaa acgggggcac      480

uuccuguuug agggagauuu cgacagcgaa aaccaguuug auaccuccau ccaagcccuc      540

uucgaguacc ugcgcgaaga cauggagguc gacauugaug ccgacucuca gaaggugaaa      600

gaaauccuua aggauagcuc ccugaaaaau ucugagaaac agagcagacu caacaagauu      660

cugggccuga aaccuuccga caagcagaaa aaggcuauca cgaaucucau cagcggcaac      720

aagauuaauu uugccgaucu guaugacaac cccgaucuua aagacgcuga gaaaaauucu      780

aucuccuuca gcaaagauga cuucgaugcc cugucugacg accucgcguc cauucugggg      840

gauagcuuug aacuucugcu caaggccaaa gcuguuuaca acugcucugu gcuguccaag      900

gucaucggag acgagcaaua ccugagcuuc gccaagguga aaauuuauga aaagcauaaa      960

accgaucuca caaagcugaa agcuguuauc aagaagcacu uuccuaauaa cuacaaaaag     1020

guguucggcu auaaccagga caaugagucc aaaaacaauu acucugggua ugucggagug     1080

uguaagacca aaaacaagaa acuugugauc aauaacagcg ucaaucagga agauuuuuac     1140

aaguuccuga agcccauucu cuccgccaaa agcgagauca aggagguuaa cgacauucug     1200

gccgaaaucg agacaggcaa uuuucuuccu aaacagauuu cuaaguccaa cgcugaaauc     1260

ccguaucaac ugcggaaaau ggagcucgaa aagauccuga gcaaugcgga gaagcacuuc     1320

ucuuuucuga aacagaagga ugagaaaggg cucagccauu ccgaaaagau uaucaugcug     1380

cuuaccuuca aaauucccua cuacaucgga ccuauuaacg acaaucacaa gaagucuuuu     1440

cccgaucgcu gcuggguggu gaaaaaggag aaaagcccuu ccggcaagac gacacccugg     1500

aacuucuuug accauaucga uaaagaaaag accgccgagg aguucauuac aagcagaacc     1560

aauuucugua ccuaucugau cggcgaaucu guccucccga agaacucccu gcuguacagc     1620

gaguauacag ugcuuaacga aaucaauaac cuccagauua ucauugacgg gaaaaauauc     1680

ugcgauacga agcugaaaca aaagauuuac gaggaccugu uuaaaaagua uaaaaagauc     1740

acccagaagc agaucucuac auucauuaaa cacgaaggaa ucuguaacaa gaccgacgag     1800

guuauuaucc ucggcauuga uaaagagugc acauccagcc ugaaguccua caucgaacuu     1860

aaaaauaucu uugggaaaca gguggacgag auuucuacca agaacaugcu ggaagagauc     1920

auucgguggg cuacgaucua ugaugaagga gagggcaaaa caauucucaa gaccaaaauc     1980

aaggccgagu acgggaaaua cuguagcgac gaacaaauca agaagauucu gaaucugaaa     2040

uucuccggau ggggcagacu uucucgcaag uuucucgaga ccgucacaag cgaaaugccu     2100

ggcuucuccg agcccgugaa caucauuacc gccaugcggg aaacacagaa uaaccugaug     2160

gagcugcuca gcucugaguu uaaauucacg gaaaauauca agaaaauuaa cuccggguuu     2220

gaggaugcug aaaagcaguu cagcuaugac ggacugguua agccucuuuu ucugucuccc     2280

uccgugaaaa agaugcucug gcagacccug aaacugguca aggagaucag ccacauuaca     2340

caagcgcccc cuaaaaagau cuucaucgag auggcgaagg gcgcugaacu ugagccggcc     2400

agaaccaaaa cacgccucaa gauucugcag gaucuguacu cuagcugcaa aaaugacgcc     2460

gaugcuuuuu ccagcgaaau ccaguaucuc ucugggaaga uugagaacga agacaaucug     2520

cggcuuagau ccgauaaacu guaccucuau uacacccagc ugggaaagug uauguauugc     2580

ggcaaaccca ucgagauugg gcauguguuc gacacgagca acuacgauau cgaccacauc     2640

uacccucaau ccaagauuaa ggacgauucu aucagcaacc gcguccuggu gugcuccucu     2700

uguaauaaaa acaaggagga caaauauccc cuuaagagcg aaauucaguc caaacagcgg     2760

ggcuucugga auuuucucca gagaaacaau uucaucucuc uggagaagcu gaaccgccuc     2820

acccggacaa ccccuauuag cgaugacgaa acagccaagu uuaucgcuag acaacugguu     2880

gagacccgcc aggccacgaa aguggcggcc aagguccuug aaaagauguu ccccgagaca     2940

aagaucgugu acuccaaagc ugagaccguu agcauguuuc ggaauaaguu cgauauuauc     3000

aagugcagag aaauuaacga cuuucaucac gcccaugaug cuuaucugaa uaucgugguc     3060

ggaaacgugu acaauacaaa auucaccaac aauccgugga acuuuauuaa ggagaaacgg     3120

gacaauccua agaucgccga uaccuauaac uacuauaaag uguucgacua cgauguuaag     3180

cgcaacaaua uuacagcgug ggaaaagggc aaaacgauca ucaccgucaa ggacaugcuc     3240

aaaagaaaca cacccauuua cacccggcag gccgcuugua agaaaggggg acuguuugac     3300

caaacaauca ugaagaaagg ccuggggcag cacccucuua agaaggaggg acccuucucu     3360

aauauuucca aauauggcgg guacaacaag gugagcgccg cuuauuacac ccugaucgaa     3420

uaugaggaga aaggcaauaa gauuagaucu cucgaaacga ucccucugua ccucgugaaa     3480

gauauccaga aggaccagga uguccugaag uccuaucuua cagaccugcu cggaaagaag     3540

gaguucaaaa uucuggugcc caagaucaaa auuaacagcc ugcuuaagau caauggcuuu     3600

ccgugccaca uuaccgggaa gaccaacgau ucuuuccugc uccgcccugc cguucaauuu     3660

uguugcucca augacgaagu gcuguacuuc aaaaagauca uccgguuuag cgagauuaga     3720

ucccagcgcg agaaaaucgg aaagacaauu agccccuacg aagaucucuc uuucagaagc     3780

uauaucaaag agaaccugug uaagaagacc aaaaaugacg aaauuggcga gaaggaauuu     3840

uacgaucuuc ugcagaaaaa gaaccucgag aucuaugaca ugcugcugac aaaacauaag     3900

gauacgauuu acaaaaagcg gcccaauucc gccaccaucg acauccuugu caaggggaaa     3960

gagaaguuca aaucucugau uaucgaaaac caguuugagg ugauucucga aauccugaaa     4020

cucuucagcg cuacacgcaa uguuuccgag cugcaacaca uuggaggcuc uaaauauagc     4080

gggguggcga agaucggcaa caagaucucc ucucuugaua auugcauucu gaucuaccag     4140

agcauuaccg gaaucuuuga aaagagaauu gaccugcuca aggucuga                  4188


<210>  4421
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4421
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggc       60

ugggccguua cagacacgga uuauaaacug cuuaaggcua acagaaaaga ccuguggggg      120

augcggugcu uugaaaccgc cgagacagcg gaagugcgca gacuccaccg gggagcucgc      180

agacggauug agcgcagaaa gaagcggauc aaacugcuuc aggaacuguu cucccaagag      240

auugccaaga ucgaugaggg cuuuuuccag cgcaugaaag aaucucccuu uuacgccgag      300

gacaagacca uucuccagga aaauacacug uucaacgaua aagacuuuac cgauaagaca      360

uaucauaagg cuuacccuac caucaaucac cugaucaaag ccuggauuga gaacaaggug      420

aaaccggacc ccagacuccu guaucuugcu ugucauaaua ucauuaagaa acgggggcac      480

uuccuguuug agggagauuu cgacagcgaa aaccaguuug auacguccau ccaagcccuc      540

uucgaguacc ugcgcgaaga cauggagguc gacauugaug cggacucuca gaaggugaaa      600

gaaauccuua aggauagcuc ccugaaaaau ucugagaaac agagcagacu caacaagauu      660

cugggccuga aaccuuccga caagcagaaa aaggccauca ccaaucucau cagcggcaac      720

aagauuaauu uugcugaucu guaugacaac cccgaucuua aagacgccga gaaaaauucu      780

aucuccuuca gcaaggauga cuucgaugcu cugucugacg accucgccuc cauucugggg      840

gauagcuuug aacuucugcu caaggcgaaa gccguuuaca acugcucugu gcuguccaag      900

gucaucggag acgagcaaua ccugagcuuc gcuaagguga aaauuuauga aaagcauaaa      960

acagaucuca ccaagcugaa aaauguuauc aagaagcacu uuccuaaaga cuacaagaaa     1020

guguucggcu auaacaagaa cgagaaaaau aacaauaacu acuccgggua ugucggagug     1080

uguaagacaa aaucuaagaa acuuaucauu aauaacagcg ugaaucagga agauuuuuac     1140

aaguuccuga agaccauccu cuccgccaaa agcgagauua aggaggucaa cgacauccug     1200

acggaaauug agacaggcac cuuucuuccc aaacagaucu cuaaguccaa ugcugaaauc     1260

ccuuaucagc ugcggaagau ggagcucgaa aagauucuga gcaacgccga gaagcacuuc     1320

ucuuuucuga aacaaaagga ugagaaaggg cucagccauu ccgaaaagau cauuaugcug     1380

cuuacauuca agaucccgua cuacauugga cccaucaaug acaaccacaa aaagucuuuu     1440

ccugaucgcu gcuggguuau uaaaaaggag aaaagccccu ccggcaagac caccccuugg     1500

aauuucuuug accauaucga uaaggacaaa acagccgagg cuuucaucac gagcagaacc     1560

aacuucugua cauaucuggu gggcgaaucu gugcucccca aguccagccu gcuguacucu     1620

gaguauaccg uccuuaauga aauuaacaac cuccagauca uuaucgaugg gaaaaauauu     1680

ugcgacauca agcugaaaca gaagaucuac gaggaacugu uuaaaaagua uaagaaaauu     1740

acacaaaagc agaucuccac cuucauuaaa cacgagggaa ucuguaacaa gacggacgaa     1800

gugauuaucc ucggcaucga uaaagagugc acaagcuccc ugaagucuua cauugagcuu     1860

aagaauaucu uugggaaaca gguugacgaa auuagcacca agaacaugcu ggaggaaauc     1920

auucgguggg cgaccaucua ugaugaggga gaaggcaaaa caauccucaa gaccaaaauu     1980

aaggccgagu acgggaagua cuguuccgac gagcagauca aaaagauucu gaaucugaaa     2040

uucucuggau ggggcagacu uagccgcaag uuucucgaaa cagugacguc cgagaugccg     2100

ggcuucagcg aaccugucaa caucauuacc gcuaugcggg agacacaaaa uaaccugaug     2160

gaacugcucu cuuccgaguu uaaauucacc gagaauauca agaagauuaa cagcggguuu     2220

gaagaugccg agaaacaguu cucuuauaau ggacugguga agccccuuuu ucuguccccu     2280

agcguuaaaa agaugcucug gcagacacug aaacugguga aggaaaucuc ucacaucacc     2340

caggccccuc ccaagaaaau uuucaucgag auggcuaagg gcgccgagcu ugaacccgcg     2400

agaacgaaaa cccgccucaa gauucugcaa gaccuguaca acaauugcaa aaacgaugcu     2460

gacgccuuua gcuccgagau caaggaucuc agcgggaaaa uugaaaauga ggacaaccug     2520

cggcuuagau cugauaagcu guaucucuac uauacacagc ugggaaagug uauguacugc     2580

ggcaaaccga ucgaaaucgg gcaugucuuc gacaccucca acuaugauau ugaccacauc     2640

uacccccaga gcaagauuaa agacgauucc aucucuaauc gcgugcuggu cugcagcucc     2700

uguaacaaga auaaagagga caaguacccu cuuaagucug agauucagag caaacaacgg     2760

ggcuucugga acuuucucca gagaaauaac uucaucuccc uggaaaagcu gaaucgccuc     2820

acacggacca cgcccaucuc ugaugacgag acagccaaau uuauugcuag acagcuggug     2880

gaaacccgcc aggccacaaa gguugcugcc aaagugcuug agaagauguu cccugaaacc     2940

aagaucgucu auagcaaagc ggagaccgug uccauguuuc ggaacaaguu cgauauuauc     3000

aaaugcagag agauuaauga cuuucaucac gcccaugaug cuuaccugaa caucguugug     3060

ggaaaugucu auaacacaaa guucacgaau aaccccugga auuuuauuaa agaaaagcgg     3120

gacaacccga agaucgccga uaccuacaac uauuacaaag uguucgacua ugaugugaag     3180

cgcaauaaca ucacagcuug ggagaaaggc aagaccauua ucacaguuaa agacaugcuc     3240

aagagaaaua ccccuauuua cacgcggcaa gccgcgugua aaaagggagg gcuguuugac     3300

cagacaauca ugaagaaagg ccuggggcag cacccccuua agaaagaagg agccuucagc     3360

aacauuucua aguacggcgg guauaauaaa gucuccgcug ccuacuauac ccugaucgag     3420

uacgaagaga agggcaacaa gaucagaagc cucgagacca uuccucugua ucucgugaaa     3480

gauauccaaa aggaccagga ugugcugaaa ucuuaccuua cagaccugcu cggaaagaaa     3540

gaaauuaaga uccugguccc caagauuaaa aucaauuccc ugcuuaagau caacggcuuc     3600

ccuugccaca uuaccgggaa aacaggagau agcuuugugc ugcgccccgc uguucaguuc     3660

uguugcucua augacgaggu gcucuauuuu aagaaaauca uucgguucaa cgaaaucaga     3720

ucccagcgcg agaagauggg caagacgauu agcccguacg aggaucuguc cuuuagaagc     3780

uacaucaaag aaaaucucug uaagaaaacc aaggccgacg agauugggga aaaagaguuc     3840

uaugaucugc uucaaaagaa aaaccuggaa aucuacgaca ugcuccugac aaagcauaag     3900

gauaccaucu auaauaaacg gccuaacucu acaacccugg gaaugcuuac gaagggccgc     3960

uacgaguuua uuaaucugaa acccaaggac cagaucauug ucaugcucga gauccugaaa     4020

cucuucugca ccacaagaga agccauugau cuggggcuua ucaagggcaa gcccgcugcg     4080

ggaguggcca cccugggcaa aaagaucagc aacccugaca auuguauucu gaucuaucag     4140

uccauuacag ggaucuuuga gaaacggauu gaccuccuga agguuuga                  4188


<210>  4422
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4422
augggcgaca ccgugaagaa cuaccacauc gggcuggaua uuggaacaag cuccgucggc       60

uucgccgcua gagacgauga caauaaccuc auccggguua aagggaagaa ugugauugga      120

acgcgccugu uuucugaggg ccagaccgcc caagaaagac ggauggcgcg cgcuagcaga      180

cggcgcuaua acagacggaa auggcgccuu agacugcucg augagaucuu ccaugacgaa      240

auuauccaca aggaugccga guuuuucaau cggcugaaac aguccucucu uagccccaag      300

gacgaacgca agcaguacuu ugggucccuc cuguucccug aggugggaga uucugaguau      360

uaccaugacg gcaaccagac aaucuaucac cugagacauc ggcugaugac ccaagaugaa      420

aaagccgaca uucgcgagau cuaccucgcu cugagacaca uuaucaagua ucggggcaau      480

uuucuugaug acacaccggu cagcuccuuc gaagccagcg accugaaacu cgcuucuuuu      540

gugcccaccc ugaacgaucu uuucgagaca auugacguua auuacgaguu uuccauggau      600

aacaucaaug aaauugagga cauccugcuc aacaauaagc ugagaaacag cgauaaaucu      660

aagcagcugc cugaccuccu gaccgaaccc aucgugaaua uugaugacaa gucccuugag      720

aaacaguuca acaaggaacg cacggauauc agcaaagaga uugccaaguc ugucgugggg      780

uccaaaauga acguuaauaa gcugcucagc uuugaguccu cuggaggcua caaaaucgcg      840

uucagcuucu ccgacgccag cucugaagau aagaugaugg agcuguccga cgugcuugac      900

gauagccgga ugaacauucu gaauauccuc aaggucaucu auucuagagu gcgccugaac      960

cagauuaucc cuagcgggau gggacugucc gaaucuaugg uggcugccua cgaccuccac     1020

aaacaacagc ugaaggaucu uaaaaagcug cucaaugucc ugcccuuuaa cgagcgguau     1080

gcuguuaaaa uugccuacgc ggccuauauc ggcaauauug acaagcagaa guucaccaaa     1140

agcgaaguga agacaaucga uaaacuuaag gagaaauccg cuaucaugaa cgugcuggac     1200

uuuaagagcg ggcauauuac caaugaggaa cucucuaacg ucaucaaggu gguuauugug     1260

gauaaauacc ugaaugacga gaacuucgaa uaucacucca ucgucgagaa gauuaaucag     1320

gauaucagca acggaucuua ccugcucaaa caaagaaaua accagaacgg caauauuccu     1380

caucagcugc accaaaugga acuugaccag aucaucgaga accaguccaa guacuauccg     1440

uuucugggcg agcucaaucc caacagcaau cgcaaccaca uugccaaaua caagaucucc     1500

gaacuggugg cuuuccgggu uccuuauuac guggggcccc ugauuacacc ugaggaacag     1560

gagaagaccu cuggagccag cuuugccugg augaaaagaa aggaggaugg cgcuaucacg     1620

cccuggaauu ucgaagagaa agucgacaag gaagagacag cgucccgcuu uauuaaacgg     1680

cuuaccguga aggauacaua ucuccugaac gaagacgucc ugccggacaa uucucuccug     1740

uacgagaaau ucaaggugcu uaacgagcug aauguuguga aggccaacaa uaaaagacuc     1800

accgucgaag agaagcaagg ggcuuuugau aaccuguuca aaaagaugaa aaccgugaca     1860

gccaagaagc ugaaaaauua ucuuguuagc aaccauaacc acaucaauuc cguggaaauc     1920

acgggacuca gcgacgucga uaaguuuaac aauucucugu ccaccuacau ugacuucaaa     1980

aagaucuucg augacaaaau ugauaacccu gaccugcucg augacuuuga ggauaucauu     2040

gaauggagca caguguucga ggaccgcaag aucuuugagg aaaaggugcg gaaagauauc     2100

ucuuggauuu ccgagagcga aaucaaggcc guugucucua aaagauacag cggcuggggg     2160

cggcugucca agaaacuucu gaauggaauu accaacaaug agggccgcaa caucauugac     2220

gaacucuggg aggagagcga aacauucaug gaggcugugu cuaagcccga agugaaggag     2280

cagaucgacg gcaucaauuc cgauuuuauu aaaaacaccg acaucgagag cguccuggcc     2340

gaugcguaua cguccccuca gaauaagaaa gcuauuagag aaacacauaa gcuggugaaa     2400

gacauccaga aggccauggg agggcaagcc cccgcuucua uuagcaucga guucacccgg     2460

aacccccagg auaaaggcca gcuuaccaga ucccgccacu cugaaguuag ccggaaguac     2520

aaggagauuu ccaaagaagu gucuaagaca cucaaugacg agcugaaaag caagaaaaac     2580

uccauggaug ucgacaagua uuaccuguau uuuaugcaga uggggaucga ucucuacgac     2640

ggaagcucua ucgauauuga cgaccugaau aacuaucaaa ucgaucauau uaucccucag     2700

uccuucuaca aggacgauag cuuugacaac agagugcuua cccacuacuc uaauaaccag     2760

aaaaagggca auaacacacc gcugaauggg cucaacauug augacaaaac gcgcgccauc     2820

uggaaucacc ugagagaucu gggccuuauc accaagaaaa aguauaacgg acucacacug     2880

aagggcaugg accagcuguc caaauacacc aagcaugggu ucauucggcg ccaacucguu     2940

gagacaagcc aggugaucaa acuggucgcu aauauucuuu cugaugaauu uuccaccgac     3000

gauacgaaga ucauugaggu gaaagccaag augaacagcc agaucagaca aguuuucaau     3060

cuguauaagg ugcgggaacu caacgacuac caccaugcgg ucgaugccua ucugaccaca     3120

uuuaucggag gcuaccugua uaaucgcuac cccaaccuua gaaauauguu cguguacggg     3180

gacuucaaaa aguuuuccgc ugacguggau aaccugaaaa ccuucaauuu ucuccacgac     3240

auuacaaacc cuagcgauaa guucgaggac cggaucuuug augaaaaauc uggagagcug     3300

auucucgacc gcaagcacgc caucgagacc auuaagaaaa ucuaucagua caaguauaug     3360

cugauuacga gagaagucag caccaaaaac ggccagcuuu ccgaucagac aguuuacucu     3420

gcuaagcgga ucaauaacgu gaaagccccc aucccuguga agaaagacag caaucccgag     3480

cuguaugggu uccgcacagg caacgucgau caucacaugg ugauuguuaa guacccgaag     3540

aaagacuccu augauuacaa gguggucgga aucccucuca gauuugugca aaaacugaag     3600

gaaaccaaag acaauaagga uuacgagaac cugcuuaauc agauuguuca gaugaagcug     3660

aaccggaaaa agcaggacuu caucgugguc guggacaaug uuauguaugg ccaacucauu     3720

caggaugggg gagaaaaaua cacccugggc ucuagcacau auaaguacaa cgcgaaacag     3780

cucgugauca auauggacaa gacgagaauc cuggaggaua accgcuaucu uaauucccug     3840

gacgaggggg aagucucuga gaagcucgug aacauuuaca augauaucau gaaagugguc     3900

aacagccggu uugaccuguu cgauauuaac aaguuuagag aaaaacugaa ugacggacuu     3960

aacaaguuca aaaauaucuc caacgagcag aaggaggaaa cccugcucaa gauucugagc     4020

ggccuccauu gcgggucugc caauguuccc aucaaagagc ugggccuuac aaccccucug     4080

ggaaagcugc aacuccccuc cggcaucgug cugagcgaag aggcuguggu cauuuaucag     4140

ucuccuuccg ggcuuuucga aaacaaagug cgccugaagg aucucuga                  4188


<210>  4423
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4423
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggc       60

ugggccguua cagacacgga uuauaaacug cuuaaggcua acagaaaaga ccuguggggg      120

augcggugcu uugaaaccgc cgagacagcg gaagugcgca gacuccaccg gggagcucgc      180

agacggauug agcgcagaaa gaagcggauc aaacugcuuc aggaacuguu cucccaagag      240

auugccaaga ccgaugaggg cuuuuuccag cgcaugaaag aaucucccuu uuacgccgag      300

gacaagacaa uccuccagga aaauacccug uucaacgaua aagacuuugc ugauaagaca      360

uaucauaagg ccuacccuac cauuaaucac cugaucaaag cguggaucga gaacaaggug      420

aaaccggacc ccagacuccu guaucuugcc ugucauaaua uuaucaagaa acgggggcac      480

uuccuguuug agggagauuu cgacagcgaa aaccaguuug auacguccau ucaagcucuc      540

uucgaguacc ugcgcgaaga cauggagguc gacaucgaug ccgacucuca gaaggugaaa      600

gaaauucuua aggauagcuc ccugaaaaau ucugagaaac agagcagacu caacaagauc      660

cugggccuga aaccuuccga caagcagaaa aaggcuauua ccaaucucau cagcggcaac      720

aagaucaauu uugccgaucu guaugacaac cccgaucuua aagacgcuga gaaaaauucu      780

auuuccuuca gcaaagauga cuucgaugcc cugucugacg accucgcguc cauccugggg      840

gauagcuuug aacuucugcu caaggccaaa gcuguuuaca acugcucugu gcuguccaag      900

gucauuggag acgagcaaua ccugagcuuc gccaagguga aaaucuauga aaagcauaaa      960

acagaucuca ccaagcugaa aaauguuauu aagaagcacu uuccuaaaga cuacaagaaa     1020

guguucggcu auaacaagaa cgagaaaaau aacaauaacu acuccgggua ugucggagug     1080

uguaagacaa aaucuaagaa acuuaucauc aauaacagcg ugaaucagga agauuuuuac     1140

aaauuccuga agaccauucu cuccgcuaaa agcgagauca aggaggucaa cgacauucug     1200

acggaaaucg agacaggcac cuuucuuccc aaacagauuu cuaaguccaa ugccgaaauc     1260

ccuuaucagc ugcggaagau ggagcucgaa aagauccuga gcaacgccga gaagcacuuc     1320

ucuuuucuga aacaaaagga ugagaaaggg cucagccauu ccgaaaagau uaucaugcug     1380

cuuacauuca agauuccgua cuacaucgga cccauuaaug acaaccacaa aaaguuuuuc     1440

ccugaucgcu gcuggguugu gaaaaaggag aaaucuccca gcggcaagac caccccuugg     1500

aacuuuuucg accauaucga uaaggaaaag acagcugagg cguucauuac guccagaacc     1560

aauuuuugua cauaucuggu gggcgagagc guccucccca agucuucccu gcuguacagc     1620

gaauauaccg ugcuuaauga gaucaacaac cuccagauca uuaucgacgg gaaaaauauu     1680

ugcgauauca agcugaaaca gaagauuuac gaagaccugu ucaaaaagua uaagaaaauc     1740

acacaaaagc agaucucuac cuuuauuaaa cacgagggaa ucuguaacaa gacggacgaa     1800

guuauuaucc ucggcauuga uaaagagugc acauccagcc ugaaguccua caucgagcuu     1860

aagaauaucu ucgggaaaca gguggacgaa auuucuacca agaacaugcu ggaggaaauc     1920

auucgguggg ccaccaucua ugaugaggga gaaggcaaaa caauucucaa gaccaaaauc     1980

aaggcugagu acgggaagua cuguagcgac gagcagauca aaaagauucu gaaucugaaa     2040

uuuuccggau ggggcagacu uucucgcaag uuccucgaaa cagucacgag cgagaugccg     2100

ggcuuuuccg aaccugugaa caucauuacc gccaugcggg agacacaaaa uaaccugaug     2160

gaacugcuca gcucugaguu caccuuuaca gagaauauca aaaagauuaa cuccggguuc     2220

gaagaugccg agaagcaguu uagcuaugac ggacugguua aaccccuuuu ccugucuccu     2280

uccgugaaga aaaugcucug gcagacccug aagcugguca aagaaaucag ccacauuacg     2340

caggcuccuc ccaagaagau cuuuaucgag auggccaaag gcgcggagcu ugaacccgcu     2400

agaaccaaga cacgccucaa aauucugcaa gaucuguaca auaacugcaa gaaugacgcc     2460

gaugccuucu cuagcgagau caaagaccuc uccgggaaga uugaaaacga ggauaaucug     2520

cggcuuagaa gcgacaaacu guaucucuac uauacccagc ugggaaagug uauguacugc     2580

ggcaagccga ucgaaauugg gcauguguuc gauacaucua acuaugacau cgaccacauc     2640

uacccccagu ccaaaauuaa ggaugacagc aucuccaacc gcguccuggu gugcucuagc     2700

uguaauaaaa acaaggagga uaaauacccu cuuaaguccg agauucaguc uaagcaacgg     2760

ggcuuuugga auuuccucca gagaaacaau uuuaucagcc uggaaaaacu gaaccgccuc     2820

acccgggcua cgcccauuuc cgacgaugag acagccaagu ucaucgcuag acagcugguu     2880

gaaacccgcc aggccacaaa aguggcggcc aagguccuug agaaaauguu uccugaaacc     2940

aagaucgugu auucuaaggc ugagaccguu agcauguucc ggaauaaauu ugacauugug     3000

aagugcagag agaucaacga uuuccaucac gcccaugacg cuuaccugaa uauugucgug     3060

ggaaacgugu auaauacaaa auuuacgaac aaucccugga acuucaucaa ggaaaaacgg     3120

gauaauccga agauugccga caccuacaac uauuacaagg uuuuugauua ugacgucaaa     3180

cgcaacaaua ucacagcgug ggagaagggc aaaaccauua ucacagugaa ggacaugcuc     3240

aaaagaaaca ccccuaucua cacgcggcaa gccgcuugua agaaagggga acuguucaau     3300

cagacaauua ugaagaaggg acugggccag cacccccuua aaaaggaggg gccuuucucc     3360

aacaucagca aauacggagg cuauaauaag gugucugccg cuuacuauac ccugauugaa     3420

uacgaggaga aagggaacaa gaucagaucc cucgaaacca uuccccugua ucucgucaag     3480

gauauccaaa aagaccagga ugugcuuaag agcuaccuga cagaccugcu cggcaaaaag     3540

gaguuuaaaa uccugguucc uaagauuaag aucaauucuc ugcuuaaaau uaacggauuc     3600

cccugccaca ucaccggcaa gacaaaugau uccuuucugc uccgcccggc cgugcaguuc     3660

uguugcagca acaaugaagu ccuguauuuu aaaaagauua uccgguucuc ugagaucaga     3720

ucccagcgcg agaaaauugg gaagacgauc agcccuuacg aagaccucuc cuuuagaagc     3780

uacauuaagg agaaccugug gaaaaagacc aaaaaugaug aaaucggaga gaaggaguuc     3840

uaugaccuuc ugcaaaagaa gaaccucgaa auuuacgaua ugcugcugac aaaacauaag     3900

gacaccaucu auaagaaacg gcccaauucu gccacaauug auauccuugu gaagggcaaa     3960

gagaaguuua aaagccugau cauugaaaac caguucgagg uuauccucga aauucugaag     4020

cucuuuuccg cuacccgcaa ugugucugac cugcagcaca ucgggggaag caaguacucc     4080

ggcgucgcga aaauugggaa caagaucucu agccuugaua acuguauccu gauuuaucag     4140

uccaucacgg gcauuuucga gaaaagaauc gaccugcuca agguguga                  4188


<210>  4424
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4424
augaacgcca auagagagau ggcuuacuau aucggcauug acauggggac cggaagcgug       60

ggcauggcca ucacagauga aaacuacaau cugcucaagg ucaaagggca ggacuauugg      120

uucguucggg aguuugaaac ggcgaacacc caaauuaagc gcagaaugca ccggaucucc      180

aaacgcagac ugcggcgcca ucaggugaga guggcucuua uucggucuua cuucgccgag      240

gaaauccuga agaaugaucc ccucuuuuuc auccgccagg acaacagcau uuauuaccug      300

gaggauaaag acuccguccu uaagacaaag gauugccugu uugccgacua uaauuaccac      360

gauaaaaacu acuauaagga cuacccuacc aucuuccauc ucagacaggc uuuuauuaaa      420

gauacaguga aggacgauga gaaucacacc cgguaugccc gccugcugua ccucgcuauc      480

auuaacaugu ucgaacauag aggacacuuu cugcuuaauu cuguuagcga ggacaucaac      540

aaagaaaugg ugaaggacgc cggcgagggg uucacacugu aucuccaaga gcggauuaag      600

gaaaucaaac cggucuccua cucugauauc cuggacgugc uuguugagaa gaauauuagc      660

cgcuccgaaa agaaggagag aaucguggaa cuguuuggaa uuaaaaagag cuguaaaaac      720

ggcauggaga ucauuaagug ccucuguggc cuggaggcga augucgccau cauguucaac      780

cuggaugaca ucgaaaacaa gcucaccauu guggugucua aagaugacua ugaggauaag      840

cuggcugaag uccagccccu uguugacgag gaauccuuug agcugcucag ccggaucaaa      900

cagcuguacg auuacucuca gcuucaaucc auucugaagg gguauaaaga gaaggaagac      960

ggagaggaua aguaccucag cgacgugaga guggaacugu augagaaaca caaggccgac     1020

cugaaacucc ugaagaaagu cuacaagcgc gaucuugaca agaaaggcaa gaucugcgaa     1080

gggaaaaaua uucagcgguc uuccaaguau aacuucaugu ucagaagcgg aaaagauggc     1140

acguacuccg cuuauaccaa uucucugaac agcuuuuguu ccaagacaau cgagaauaag     1200

uaccgccgga acaugaaaaa uagagaccgc gccagccucu acgauaccau caagaaagac     1260

cugucuggga uugagaacga ugacgugaag aaaauccuuc gggauauuga aacagagaag     1320

uuccugccua aacagaucac cggagaaaau ggcguuauuc ccaaccagau ccaugcgaag     1380

gagcucaagg ccauucugaa aaaugcugag aagcaucugc cuuuucucac ggaaaaagac     1440

gauuccggcc ugacagugag cgagagaauc aucucucuuu uccgcuauug cauucccuac     1500

uauaucgggc cugucggaaa ggacagccgg accggcuggg ccuccauuaa agaagagggg     1560

caaaucuuuc cguggaacau ugaacagaag aucgaugugg acaagacauc ugaggaguuc     1620

aucaccagac ugauuaaaac cuguacauac cucagcaaug aaaagguugu gcccaaacag     1680

ucccuggucu augagcgcua cugcgugcug aacgaaauca auaacauuaa gauccgggga     1740

aaaagaauug acccucaacu uaagcaggag aucuuuaagg aacucuauau cggcagcaaa     1800

ugugggaaga aagucacgcg gaagaaccug ugccaguacc uggugaauaa aggagcuauu     1860

gaggaggauu cugaaaucuc cggcauugac aaggaucuca acaccuacau gagcucuuau     1920

ggcaagaugu accagaucuu cggggacaaa auuaagcgcg augacaucaa agagguugcc     1980

gaagagauca uuuauuacgg aacaaucuau ggcggggcca aggaaauguu uaaaaagaaa     2040

auugagcaau acguguccga ggauauccug gacgaagcuc agaugaaucg gauucuugga     2100

uucaaguuua aggauugggc gagacugagc aaaaaguucc ucuaucugga gggcuacgac     2160

aaagggaacc agaaucagcu gaccacacuu augcaagccc ucugggauua caaccugaau     2220

accauggaac ugaugaacuc ccccauguuu acguucaagg agacacucga gaaacagaag     2280

aauaagaugg aaaaaguccu gugugacuuc gaguaugaag aucuugacga guacuauuuu     2340

ucugcuccug ugaagagaau gguuuggcag accaucgccu gccugaaaga agugguccag     2400

auuaugggcc acaaccccaa gcgcaucuuc aucgagaugc cgcgggccga cgaggaaaaa     2460

ggagagcaag gcagaaaggc uagccgcaag aauagacucc ugucccugua cgaagagauu     2520

gaagaugagc ggccuuggaa aaaggagauc gaaaacgccg cggagucugg gcgccuuaau     2580

agcaaaagaa uguaucucua cuaucugcag augggaaagg acgcuuacac cggcgaagau     2640

auugacaucg aggaucuguu ugacaacucc aaauacgaua uugaccacau cuauccccgg     2700

agccucacag augacaauuc ucuggaaaac aaccuugugc ugguguccgc caccaucaau     2760

caggauguua agaagaacga cuacccuauu cccuaugaca aaauccgcgg gaauagcaag     2820

gucagagagc ucuggcgguu ccugcacgag aaaaaguuua ugaacgauga aaaauacaau     2880

cgccugaugu cugacgugcc ccuuacacag gagcaacucg ccggauauau ugcuagacag     2940

cugguggaaa cggcccaggg caccaagggg aucgcugaua uuaucaaguc cgucaucccu     3000

gacgccgaua uuaucuacag caaagcgaag aacgugucug acuuccggaa acgcuauggc     3060

augcucaaga gcagacugac aaaugauuuu caucacgcca aagacgcuua ccugaacguu     3120

guggucggaa auguguacuu caccaaguuu acacaaaacc cgcggaaaua uauuaaggau     3180

gagaaugaca ucgagaacaa gaaauacaau uauaaccuuu ccaagaugua cgauaaagac     3240

guuauuagaa auggcaaggu ggccuggauc gcucagaacg ggaaaaaggc cggaaccauu     3300

cagacgauca agaaagucau ggaucgcaac acccccauca uuacacggca ggcguucgaa     3360

caaagaggcg agcuguauga ccuccagccu aucagcaagc auaaagccaa gaaagaaaau     3420

uacgugccca uuaaggcuac caacgagaag augaaagacg uuacaaagua ugggggauuu     3480

accucucuga auccugccua cuucguguuu gucgaauacg gcuccgcuaa aaagcgccag     3540

cggugcuucg ccguggugca cagcuauugg gccuccucua ucaaaaacaa ugaggaucug     3600

gucacgcauc uuaaggacag aggguacgag aaccccauug uuaucgcuga uaagaucaaa     3660

aagaauagcc ugauuaaaua uaacggcuac uuccucuaua ucgugggaau ggacgcgcgc     3720

aaacaggugg aauuuuccaa ugccacaucu augugucuga acaauaacuu cauucaauac     3780

auccagaaaa uugagaaggc uaauacccuc aucgccgaag ccaaaaagcg gagaccguau     3840

aucgaugaga gcauuacagc ugaacgcaac aucgcccugu acaaugagcu uaagaaaaag     3900

caccuggacu ccauuuuuaa aaaccacccu aagggcaucg ggucuguccu cgagagcgga     3960

gaaggcgcgu ucgugcagcu gucccuggag cagcaaauug ggauccuuua cagcauuauc     4020

caguuuucuu ccuucaaaaa gggaaccuuu agccugaccc ucaucggcgg gcccaaggaa     4080

guuggccgga uuagaaucuc uggaaauaug ccuaaagcuc gggagcugaa gcucauucau     4140

uauuccauca caggcaugua caaaaaggaa auuaaccugc uugauuga                  4188


<210>  4425
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4425
auggagaccc aggccagcaa ccaacugauc uccucucacc ucaagggcua ccccauuaaa       60

gacuauuucg uggggcugga uaucggaaca agcuccgucg gcugggcugu uacgaauaag      120

gccuacgaac uucugaaauu uagaucucau aagauguggg ggagccggcu guucgacgag      180

ggagaauccg cgguggcucg cagaggcuuu cggucuaugc gcagacggcu ggagcgcaga      240

aaacuucggc ugaagcuccu ggaagaacug uucgccgaug ccauggcuca gguggacccu      300

accuuuuuca ugcgccucag agagagcaag uaucacuacg aagauaaaac aaccgggcau      360

uccagcaagc acauucuguu uaucgacaaa aacuauacag aucaggacua cuauaaggag      420

uacccgacca uuuaccaucu ucgggucgcc cugaugaaau cuggaacaga ugacauccgc      480

aaacucuucc uggcugugca ccacauccuu aaguauagag gcaauuuucu guacgaaggc      540

gccaccuucg auuccaacgc gagcacgcuc gacgauguuc ugaaacaggc ccuggagaau      600

auuaccuuua acugcuucga cucuuccgcu gccaucggga gcauuggaca aauccucaug      660

gagcugggca agacaaaauc ugacaaggcu aaagccauug aacggcuugu ggauaccuau      720

aucauuacag acgaggucga uaccuccggg aagacgcaga aagaacaggu gaaggaggac      780

aagaaacgcc ucaagagcuu ugcgaaucuc guucugggac uuuccgccuc ucugacagau      840

cucuucggca gcguggaaga ccuggaugag gaccugaaaa agcuccagau caucggggau      900

accuacgacg agagacggga ugaccuggcu aaagucuggg gagacgaaau ucauaucauu      960

gaugacugua aguccgugua ugaugccauc auucuuauga gcaucaagga gcccggccug     1020

acaaucucug aauccaaagu gaaggcuuuc aaaaagcaca aagacgaucu cgccauucuu     1080

aagagccuuc ugaagucuga cagaagcauc uacaacacca uguuuaaagu cgaugagaag     1140

ggccuccaua auuauguuca cuacauuaaa caagggcgca ccgaagagac auccugcaac     1200

cgggaggacu ucuacaagua uacgaaaaag gugguggaag gacugccuga uucuaaggac     1260

aaagaguaca uccugagcga aauugagcuc cagacccugc uuccccugca gagaaucaag     1320

gauaauggcg ucaucccuua ucagcuccac cuggaggagc ugaaagugau ucuugccaag     1380

ugugggccca aauuuccuuu ccucaacgag guugcugacg gauuuuccgu ggcggaaaag     1440

cugaucaaaa ugcuggaguu ccgcauuccg uacuaugucg gcccccucaa uacacaucac     1500

aacguggaua augggggauu ugccugggcu guucggaagg ccagcggcag agugaccccu     1560

uggaacuucg acgacaagau cgaucgcgaa aaaucugccg cugccuuuau uaagaaccug     1620

acaaauaaau gcaccuaccu ucugggcgag gacguccucc ccaaguccag ccugcuguau     1680

ucugaguuca ugcuucucaa cgaacugaau aacgugcgga ucgaugggaa accucuggag     1740

aaggcgguca aggaacaucu cauugaggcu guguuuaaac aagaccacaa gaaaaugacg     1800

aagaauagaa ucgaacaguu ccugaaagau aacaauuaca uccccgagaa gcauaagccg     1860

gagauuacag gacuugacgg cgaaaucaaa aacgaucugg ccuccuaccg cgacaugguu     1920

cggauucucg ggaauaacuu ugaucugagc auggccgagg aaaucauuac cgacaucacc     1980

aucuucggag aguccaagaa aaugcugaga gaaacacuuc gcaauaaguu cucuagccgg     2040

cucgaugacg aggcuauuaa aaagcugucc aagcugagau aucgggauug gggccgccuc     2100

ucuagaaaac ugcuuaacgu gaucgagggg ugugacaagg ccggcgacgg aaccccugaa     2160

acaauuauca uucugaugcg gaauuuuagc uacaaccuca uggagcugcu gggcgauaaa     2220

uucuccuuua uggaaagaau ccaggagauc aaugcuaagc ugauugaagg gcaaaucguc     2280

aacccccacg acauuaucga ugaccucgcc cugagcccug cggugaaacg cgccguuugg     2340

caggcucugc ggauugugga ugagaucguc cacaucaaga aaacgcuccc cgccagaauu     2400

uucguggagg ugacccgcuc uaacaaggcu gaaaagaaaa agaaagacuc cagacagaag     2460

cggcugagcg aucuuuaugc cgcgaucaaa aaggacgaug cccugcucuc uggacugaca     2520

aaugaggaau uuggcgggcu gaaguccgga cuugcuaaau acgacgaugc cgcucugcgc     2580

agcaagaaac ucuaucugua cuauacccag augggcagau gcgccuacac aggggagguu     2640

auugaccucu cucaacugaa caccgacaau uacgauaucg accauauuua uccccggagc     2700

cuuacgaagg augacuccuu cgauaaccug guccucugua aacgcaccgc caaugcucaa     2760

aagagcgaca cauacccuau cucuaaggag auucagaaag cgcagaaggc cuuuuggauc     2820

uuccugaaac agcagggccu gaucuccgaa agaaaguaug agcggcuuac ccgcauuaca     2880

ccgcugaccg cugaugaccu cagcggauuu aucgccagac agcuggugga aacgaaccag     2940

uccgugaaag ccgcuacaac ccuccugcgg cgccuuuacc ccggcgucga ugugguuuuc     3000

gugaaggccg agaaugucac agacuuuaga cacgauaacg acuucauuaa ggugcggucu     3060

cugaaucauc accaccaugc gaaagaugcu uaucucaaca ucguuguggg gaaugucuac     3120

cacgaaagau uuacccgcaa cuuccgggcc uucuuuaaga aaaauggagc caacagaacc     3180

uauaaucugg cuaagauguu caacuacgac gugaaaugca caaacgccaa ggacggcaag     3240

gcgugggcug uuaaaacgag cauggauacc gugaagaaaa ugauggacuc caaugauguc     3300

cgcgugacaa agcgggugua cgagcaaaaa gggcagcugu cugacauuaa ccuuaccaag     3360

gccagcaaag ucaaggccga uaucuaucug ccuaucaagg cuaaugaccc cauuaugucc     3420

cagaacauga aauacggagg cuauucugau aucaagaaug ccuacuauac aauuaucgag     3480

uacagcgaca aaaagaacaa uuccauuaag gcuuuuaucc cuauccccau uuucaucgcc     3540

accaagacgc ucacaaagca ggcgcugcuc gaauauauua aaaccguucu gccgaaggcc     3600

aacaaucuua gaaucauuua caaaaaggug uacaacgauc aacugcugaa aaucgacggg     3660

uuuaauuauu accucggcgg acgcagcaac gaucgguucu guauugacaa ugcuauccag     3720

cugaucgugc cucuugagau ucaguuuuau aucaagcugg ucgaaaaguu caccgccauu     3780

aucucugaga acaaaaauau ugcucucaag gccuccagcg ugaaaucuag aaaccccauc     3840

aacaaucaag uugaaaacau cacacuggag uacacccaga agcucuuuga uuaucuggug     3900

uccaaauuca augagcuuau uuaccugaag augcgcggca acaagcugac agacgucaau     3960

aacaucgggc ggaaacaguu uauuaagcuc agccuggaag agagaugcaa agcccuugcu     4020

gaacugcuca auaucuucac gaacaugaau aagaaacugu cucucuccac cauuaacgug     4080

agcgcguccc ggaagguugu gaauuucaaa aucagcucuc uggacgaguu uaagaucguc     4140

aacgaaagca uuacaggacu uuauucccau gagaucacca uuguguga                  4188


<210>  4426
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4426
augaaccuga gaaauaccac agacgaguac aacaucggcc ucgauauggg gacgaauagc       60

gugggauggg ccgucaccga caagguuggc gcuauugccc acuucaaagg gaugcccaca      120

cugggauccc ggcuuuuuga ugcggcucag accgccgccg aagcucgcgc ccauagaggc      180

caacggcgca gauaugugcg gcgcagaugg cggcuggacc uccugcaguc ucuuuuccag      240

gaugagaucg acaaggugga uagcgaauuu uucauucgcc ugagacaguc ccggcuccac      300

gucgaggacc gcgaagaugg gcaugcugac uacaaauggc cuaucuuuaa cgauucugac      360

uucacagagg uggauuauua caagagauuu ccgaccauuu aucaucugcg gaaauggcug      420

aucgagacag accaaaaggc cgacauccgc cucauuuacc uggcgguuca caauaucgug      480

aagcacagag gaaacuuccu ucgggaaaau aacaaaaauc ugagcuccag aaacgccgau      540

cccgcugagg ccgucggcca ccuccgccug gcucuuaaua acuggugccu ggaacggggc      600

uaugagugug ccuggaaguc ugggggaaau gacaacgcga uucucaaugu gcugaucgau      660

ggcgagaaaa gacgcagcga ccagguucgg gaaauugcca agcugaccgg gaucgauugc      720

ggagacgcua aacaguccag acaguguaac gaggccauug cuaaggccau cgugggccuc      780

aaggccgaaa ugaaaaacau cuuuggggag uucccugcug aaaagacgcu gauuuaccuu      840

agcucugaug acgaugucga gguggugcgc gacgcgugcc ccgaugacuc cgucgagcug      900

uuucaagccc ucugugaacu guacgcugcc uauauccuuc agggacugcu cagcuacgcc      960

ccuggccaga ccauuucugu uaauauggug aaaaaguaug accgguacgg cgaggaucug     1020

caucugcuca aagaacugac aagaaccuau gcuuccaagc gcuacgacgc cuucuuuaga     1080

ggggagacau augaaggaag cucugauuac guggucgaca aagcgcaggg cuacaccaga     1140

uauaaccuug ggacgacaca acuguccuac gaugacuucc agaaggcugu gaaggaacuc     1200

uucaaaggaa ccgaugcccu gcaggacgag cgcuaucaag gcaugauggu ugccuuuggg     1260

gaacagcggu uccuuagacg gcugaagaca agcgauaaug gauccaucua cuaucagcuc     1320

caccuggagg aacugcaugc uauucucgag aaccagggca aacacuaccc cuuucugcuu     1380

gccgaagagg acaagaucga gucucuggug agcuuccgca ucccuuauua cgucggcccg     1440

cucaccagag aaaaugcugc cguggggcac gauggaaaag agcgguuugc gugguccagc     1500

agacgcgacg gcauggaaca ugccguuauu acccccugga acugggagga gaucauugau     1560

cgggacaagu cugcugaaga cuucaucauu agaaugacag gggauugcac guaccugcaa     1620

ggagagccug ugcugcccaa guguucccuu cucuaugaag aguuuugcgc ccugaaugaa     1680

cugaacggcg cucacuggac caucgacgag gaugacgagc gcagauucga ugccgcgcag     1740

cgggaacgca ucauguguga ccucuuuaga agcacacgga aagucaccua caagaaagug     1800

ucugauuggc uggagcgcga agacagcuuc gucggggcca gagugcaugg cggacagggc     1860

gagcacgggu uugaauccca gcuuggaucu uauauuuucu uuugcaagga uguuuucaaa     1920

acagacagcc uggaugaggc ugacuacccu augaucgagc aaauuauccu cugguccacc     1980

cuguucgaag aucgggacau ucugcgcaga aagaucgagg aagccuaugg cgaccggcuu     2040

acgacagagc agauuaaggu gaucuguaaa aagcgcuuug cugggugggg aagacucagc     2100

aaaaagcugc ugaccggcau caaagucgau accgacacag ggcggaaguc uauuaucgau     2160

gugcucagag aaggcaaucc caacgacgga cagcgcuccc ggaccauggu uuucauggag     2220

gugcugagag augagggccu uggguuucag aaggccgucg acgauuucaa ucgcgacuac     2280

uuuagcgaac auaacaaugg acugauggau gugaacgacc ucccgggcuc uccugccauu     2340

cggagaucca ucaaucaagc ugugcgcauu guugaugaga ucgucagcau cgcgaaaaag     2400

gaacccgcca acauuuucau cgaggugaca cgggaggaag acgacccucg gaaaaagggg     2460

aaacgcacga agcggagaua ugaugcuauu aaauccgccc uggagguguu uaagaaggac     2520

gauccccagc uguggaaaga acuuaccaag aaagacucua acaauaugga ugagcggcuc     2580

agccuguacu ucaugcagcg cggaaagugc auguacuccg gcagacccau caaaauugag     2640

gagcugcaca cagggaagua ugaggucgac cacaucaucc cucggucuua cauuaaggau     2700

gacagccucg aaaacaaagu gcugguuuau cgcgaggaaa aucagcauaa gaccgauaca     2760

cuucugaucg acuccagcau uagacggaaa augggcaagu acuggagaau gcuccacaac     2820

gccaaacuga ucggagauaa gaaguuucgc aaucuguucc gggaccauau ugaugacaaa     2880

accaugaagg gcuuuguggc uagacaaauc gucgagacgu cucagauggu gaaacuuguu     2940

cagacccucc uggaagcccg cuauccgggg gcgcggaucg ugcccgucaa ggcuaacauu     3000

ucccacgacc ugagagaugc cgccggacuc gugaaaugcc gcgaggcuaa ugacuuccac     3060

caugcccacg augcguuucu ggcuugucgg guuggccuuu ucauccaaaa gagacauccu     3120

agcguguacg acaaccccau ugggcuggcc cacaucaugc gcgauuaugc cagagcucag     3180

gcccgggagu acaagaaauc ucauuaccuc gcucacacaa agggauucau ugucaauucc     3240

uuucugagcu cuggcuucga caaagaaacc ggggaggugu uuaaggauga cugggaugcc     3300

gaagcggagg uggaaggcau ccgcagaguc cugaacuauc ggcagugcuu cauuagccgc     3360

augccuguug aggccacagg aaccuuuugg gaccagacga ucuacucccc caaauguggc     3420

aagaaacuua cacugccgcu caagagaggg cuggauccug accgguaugg aagcuacucu     3480

agagagcaau ucgcuuacuu uuucaucuau gaagcccgcg aucucaagca cgagcggcau     3540

auucugcagu uugcucaggu gcccgugaga gucgccgccc acaucgcuaa agacccuucc     3600

cagcuugaaa gcuaugcggu gaagcuggcc ggccaagcua aucucgccuu cguucgcauu     3660

gugcggagac ggauccugaa aaagcagcug cuugagcuga acggggaccg ccucauuauc     3720

accggaguca gagaggugcg gaaugccaca gaauuugcuu ucucccugga ugaggaaacc     3780

cucaucgagc gcugcguuca gauugccaaa gugggcucuc ugaucggcgu cgugaacgac     3840

gaagaugagg agcagcuuag cgcggaccug aaugaacucu ucuccucucu gguuaagaga     3900

gggcccaagc uguguaaccg gcuuuacgcu caacugaaac ucgauaagua ccugagcaaa     3960

uuugacgagc ucacccugac agauaaggcc aaaguggucc uucagcugau ugccaucacg     4020

aacggaucca uuaauauggc ugaccugucu gcccucggca gcuccaagua ugcggggugc     4080

augcaaguga gcuacuucaa gcagcugaac gcuccuaucg gagaagugaa uaucauugau     4140

cagucuguca ccggcauguu ugagcgcaga acacauaucg ggcuuuga                  4188


<210>  4427
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4427
augaaggaga ucaaagacua cauuaucggc cuggauauug ggaccaacag cugcggauau       60

guggucacag acaagcagaa uaacauccuc aaacugaagg gcaaaacggc cauuggggcu      120

agacuuuuca aggaaggaca agccgcggcu gaucggcgcu ccuuuagaac cacacggcgc      180

agacuggccc ggcgccacug gagacucggc cugcuugagg aaaucuucga ccccgagaug      240

gaaaagaucg auccuaauuu cuuccggcgc cugaaagagu cugacuacag cccgaaggau      300

uccagaaaac aguuuaacgc cauuguuuuc aagucuauca aagcugacaa gcgguuuuau      360

aagaaauacc ccaccauuua ucaucuccgc aaugcccuga ugcacgauaa ccagaagcau      420

gaccugagag agaucuucau ggcugugcac cauauuguga aauaccgggg gaauuuucuc      480

cgcgaagaua gcgucaacgc cuucaaggcg gccaaauuuu cccugagagg agaggacggc      540

aucggcccug uggauaaacu uaaugaccug cucaaagaaa uuuauucuga guacagcccc      600

gagcuggcua caaacaaucu uuccaagauc gaagacauca uuaaggauaa aaaccuguac      660

aagcaggaca aacucaagca aguugccacc cugcugauca aagaggcuga uagcaaggac      720

aaggccaaac ucaauaagga uauugcgaaa cagguggcca acucuuucau gggguauaug      780

uuucggcugg acacacuuuu cucccugacc gaugucgacg ugaaggauua caaacucaag      840

uucagcgacg cuaauaucga ugaaucucug gacgaccuua cgucccugcu caccgaugcc      900

cagauugagu uuguucugga acugcagagc aucuauaaca caaucgugcu caacgagauu      960

gucccugacg gaaugucucu guccgagagc augaucaaga aauacgauga ccacaaggaa     1020

gaucuuaaaa uuuucaagga guauaucgac ucccugucug aucaaaagaa ggcuaagaaa     1080

cuccugagcg ccuacaaucu uuaugugaac uacagaaagu ccgaccuggu ggaagccaaa     1140

aaucucuuua agaacaaaaa ggucggcgau aaaaauuucg cugacguuau uagcaacuuu     1200

gagguguucg ggaaguuugu gucugauaau cuggacgauu ccgaacuggc gaacaagauc     1260

aaagcccgcc ucgaccuggg agaguuccuu cccaagcagc ggaccaauca gaacggcguc     1320

aucccuuacc agcugcacca aguggagcuc acacagauuc uggaaaaaca ggggaaguau     1380

uacccguuuc ugaucacccc caauccuguu gagagccaua gaaacaaugc ucccuaugaa     1440

auuucugagc uugugagcuu ccgcgucccu uacuaugugg gaccccucau cgacaaccaa     1500

uccauuaaag auaagcagaa uaagaacaaa uuugccugga ugguucggca gaagcagggc     1560

caaaucacgc cguggaaccu ggaagagaug guggacacaa ccgagucugc caaucaguuc     1620

auuaaaagaa ugacacgcaa ggauaccuac cugcucgcug aagacguccu gccuaaaagc     1680

ucccuugugu aucagaaguu uaugauccug gaugagcuca accggaucaa gauugacggc     1740

aaaaagcuga ccagcgaacu gaaacacgau aucuucgaga aacuuuucaa aaagcagaag     1800

ucuauuaauc ucgacaaccu gaaaaauuac cugcucgucg aggggaacau ccccggacug     1860

auugaaggcc uuuccgaugg gaucaauuuu aacaauagcu ucucuacaua caucgacuau     1920

agaaacauuu uuggagauga gaucgacaau ccuaacaagc aagccgauuu cgaaaagaug     1980

auugaguggu ccacgguguu ugaagaccgc aagaucuuca aacggaagcu gaaagagauu     2040

accuggcuca caagcgagca gaucaagcag guuuccucua agagauacag cggcuggggg     2100

cgccugucca aaaagcugcu uacccagauc acagacgaaa auggagugaa cauucuccaa     2160

cggcugugga augagcccga aacccugacg gagguccucg cgaaccccgu gaucaaaaga     2220

aagauuguug aagcuaacuc uagcuuuaca aaagccaagg auaugaagga cauccuggug     2280

gaggccuaua ccuccccuca gaauaaaaag gcuauucggc aggucaugcg cgugguggau     2340

gacaucaucg ccgcugccca uggcaaaaag ccgagccaga uugcgaucga guucgccaga     2400

gaagaucaag acaaccccca gaugaccaaa ucucggaagg cucagcuuga ucaaauuuac     2460

gccaagaucu ccgacgaguu ucuggaugac gcuguuaaaa augaacucaa gaacaugaaa     2520

gauaauaagc cucuggccaa agacaagguc uuccuguauu uuaagcagau gggcagagac     2580

gcguacagcg gggagaaacu uucucucgau aaccugcagg acuaugauau ugaccacauc     2640

uacccccagu ccuucauuaa ggaugacagc uuugauaauc gcgugcuggu gcaacgggcc     2700

uacaacaaug gaaaaucuga caacgucccu gugcuccugu ucggcaauaa gaucgcugcc     2760

gggcuuggaa ucacaauuag acagaugugg aaaaaguggc uggaacucgg ccugaucagc     2820

aagcgcaaau ucaacaaucu gauuaccgau cccgagaaga ucuccagaaa cauggcuagc     2880

ggguuuguua aucggcagcu uguggagaca ucucagguca uuaaacuggu ggccaacauc     2940

cuccaagcca agcugccgga cacggaaauc auugagguua aggcuuccua uaacagcauc     3000

cuccgcaagg aauuuaaucu guacaaaucc agagagguga acgauuauca ccaugcgauu     3060

gacgccuacc uuaccacaau cgucggcaau uaucuguacc agguguaucc uaagcuccgg     3120

cccuacuuug uuuacggaca guucaagaaa uuuggcaagg cugagaacca ggaagaugac     3180

ccugugaaaa agauuaaaaa guucaauuuu aucuaucaac ugaucugggg gaaggacgau     3240

gccauuugua ucucucacac caaccagcag gucuucagca aaaaggacau uaucgauaaa     3300

cugacaaccg ccuacaauuu uaaguauaug aacauuuccc augcugugua cacgcgcaau     3360

aacaauaugu ucaaacaaac ccuuuuuccc aucauugaga gaacaaccgc caagacaaag     3420

ucucugaucc cuaaaaagcu cgaccggccc accgauaucu auggaggcua cagcaacaau     3480

aacgacgcgu aucuggcucu cgugaaaguc gauaagaaac gcggguccga auacagaguu     3540

gugggcauuc cgaugcgggc cgccgcuaug cugaaggccu cuaagaauua cgacgaggau     3600

cuucgcaaag ugaucgaacc uauggucaug uucgacaaga acggaaaagc gaagagaggg     3660

auugagggcu uccgggugcu gguuggaaaa augcccuuua agcaggcugu gcucgauggc     3720

aauaaaaagu ucaugcugac ggggacaguc gacaccccca accugaagca gcuugugcug     3780

agccagcaaa acaugaaaua uauccucgau uacauugagg acguugacug cagaaagcac     3840

aaauuuggag auaaggugca gaauccugac aaaugucugc ucgaagucuu cgaugagauc     3900

aucgaaauug uggacaagua uuuugagcug uucgauuccc gcgacuuucg ggaaaaacuu     3960

agaaacagcc gcaauaaauu caucaagcug gaggccucua aaaaggccga uacaauucug     4020

gcuauccuca aaggccugca ggccaagucc acccgggcua ccauuaagga gcuuaacauc     4080

agcgacuuug ggagaaucau uacaucuucc aucagcccgg aagcccagcu gauuuaccaa     4140

ucucccacgg gacucuucga gcgccggguu aaagugaagg aucuguga                  4188


<210>  4428
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4428
augagcaaga aauaccagaa caccaaguau uacgugggcc uggacaucgg gacauccucu       60

gucggauggg ccguuacgga ugcuaauuau gagcuccuga aagugaacgg ccacaagacc      120

ugggggagca gacuuuucac agaagccgag accgcgcugg aacggcgcac acauagagcu      180

acccggcgca gacuccaacg gcgcaaacag agacuggacc uucuggauga gcucuuugcc      240

gaggaacugg ccaaggugga cccccaguuc cugcuccggc ugaaggaguc ccaguaccac      300

auggaagaua aaucuacaga cgauaaguau guccuuuuua augaccaaaa ccgcaccgau      360

ggagaguucu uuacggaaua cccuaccauu uaucaucuga gaagcgagcu caugcagggc      420

aaaaaggagg acauccgggc uguguaccug gcccuucacc auauuguuaa auaccgcggg      480

cacuuccugc ucccgggaga uaauuaugac augcagggca accuguccga acugaucaag      540

agacucuuua aguucauuga ggaaacacug uuuaucgagg aucuuggccu gaccgacgaa      600

auccagucua aaauugagga gauccuccug acaaagacca aaacgcggcu ugaccgccaa      660

aaggaaauug ugaaacuguu caauacaaag caccagaaac ucgaugacaa gcagaagaaa      720

cugcuggagg cuaucuuuaa gcucugccug gggcuuaaag gaaacauggu caauauuuuc      780

ggcgauaagg gggaauacgg aaucgacuuu accgaugugg aggacaaaaa gaagaaaguu      840

gaauuuagcg ccgauuauga caacaauauu aacgauuacg uggacuucuu uggcgaguau      900

uucgagcugc ucgaaaucac aaagaaaauc aaugauaccg ucauuuucuc cgagauccug      960

guggacggga agggauucag cgaaucuaaa gugauuuccu acgagaagca ucagcaagac     1020

cuuagaaagc ugaaaacccu ccucaagagc cuggauaaaa cacugggcua uaaguacggc     1080

gaagggcugu accagaaaau ccuucuggac gaugucaagu cuaacuaugu uaauuacucc     1140

ggaaacagcc agaagcucaa aaaguguacg aaagacgagu uuuauggcuu cauuaagucu     1200

aaacugaacu ccgugaccaa gaaagagaag gugagcgaag cgcaacagaa ggagaucgcc     1260

cagauccugg cugauaugga auuuggggac uaccuucccc uccagcggac aaccgauaau     1320

ggagucauuc cuaaccaacu gcacaaaaag gagcugaucg agauuaucga acagcagaca     1380

aaauaucaug acuucuuuaa ugauguggac gagaacggcc ucaccauuaa ggauaaaauc     1440

aucucccuga ugacguuccg gauucccuac uauguugggc cucuuaauga cuaccacaag     1500

guggccgaug gaacaggcca cgcuuggauc gaaaagaaaa aggacggcaa aauuuacccc     1560

uggaacuuug acgagauggu cgauaccgaa gccaccgcgg agaaguucau caaaaaucug     1620

acaaacaagu gcaccuaucu caagggggag gacgugcugc cuaaagaauc uaagcuguac     1680

agcgaguuua ugcuucucaa ugaacugaac aauauuaaaa ucgauaacca gaagcuggac     1740

ccgaaacuca agaugaagau gguuuccgag cuguucauga aaaagaauga aaagauuaca     1800

aagaaaaaga ucguggauua ucuucgcagc aucggacaag ccaacggcaa ugagaugguc     1860

ucugggauug accgggagau caaauccgau cugaagacga uucauaagau ccagcacuac     1920

uaucaggacg cuagccucga aauggccgag aacauuauca gauggaacac caucuucaau     1980

gauucuacaa gcgacauucu gaaagauaag aucaccaagg cuuacggaga cgugcugaca     2040

cccggccaga uuaaggggcu uuccaaccuc gccuguaaag auuggggcaa gcugucugaa     2100

aaguuucuga ccggaauccg cugcgacguc ggcgaguuca aaaauauuag caucauccaa     2160

gccaugcggg acacgaacaa uaaccucaug gaacugcuuu ccaauaagua ugaguuuaug     2220

gauaaaauuc aggaguacaa caaggaauac uauggggacu ucaccuuuaa agcugagccu     2280

agcaucaugg augaccugua cgugggaccc ggcccuaaga gagcgauuug gcaggccguu     2340

cgcaucgugg aagagauuaa gcggaucaug gggcaugauc ccgagaaaau cuuccucgaa     2400

acaaccagaa caaaucgcgc uccgaagguc aaaaacacca agcgguauga cgcccuggag     2460

aaaaaguacg aaucugugaa ggagcacgaa uaugccgagg uuaaaaagga guuugaaauu     2520

gagaaaaaug auucccugaa caucaagaaa cuuuuccucu acuuuacgca gcugggacau     2580

uguauguaua gcgacgaaaa gauuccuuac aaugagcugu ucacaaacag auacgauauc     2640

gaccacauuu auccccgcuc uaagacccgg gauaauucca uggacaaccu cgugcugguc     2700

aaagcugaau gcaacagaga caagaaagau aauuacccua ucaaacuuaa aacaaacauu     2760

caacagaagu gucaggugcg gugggccuau cugcgcgaca aaaagcucau caccgaggag     2820

aaguacaaua gacuggugcg gaaaacaccc cugaccgaag augagcuuag ccaauuuauc     2880

gcgagacagc ugguuuucac gucccagucu gucaaggcua ccgccgaagu guuuaaacgc     2940

cucuaugagg acagcaagau uugcuacgug aaagccgaaa acgucuccga uuuccgguau     3000

ggcucuacaa gaaagaugag caaggacgau cagccccaac cuuucaucaa aaaucgcgac     3060

gugaacgauu uucaccaugc uaaggacgcc uaccugaaua uuguuguggg gaacgucuac     3120

gaugugaaau ucacccagaa uccgacaaga uuuaucaagg cuaacaaaga cuauucccuc     3180

aauuacauuu uccgguaucc cguuaacaag aagggcgaga auguguggga uccugagcag     3240

agcaugaaaa ucgucgaaaa gacccugaaa ucuaacgacg ugcgcguuac gaaacuugug     3300

aaacccauca cauccccuau uagcaaugag acccugcaca aggcccucaa guguaaaccc     3360

ggaucuuacc ugccgagaaa guccagcaaa ggcaccccuc uggcggacgu cacaaaguau     3420

gggggauacg agaaaaucaa gaacgccuau uacucuauuu acaaauauac cuacaugaag     3480

aagaaagaau gcauggagga auuucuucug gugcccaucc cuauucaucu cgugaacaau     3540

gaugaggaac ugcccaagua uaucaacgac aaaaucgcug agcgggccaa gaaaaagaag     3600

cucgaaacag ucgaguguau uguuaaagag cuguaccagg gcacgcuugu gcgcaucaau     3660

ggguaugauu acagaauugc uggaagcucc ggcaagcaau ucgaacugca caacgggauc     3720

aaucucauug uggagccuug ggucgaaaaa gugcugaagg agaccgacaa auuuguuaga     3780

aagagcaacu cuaagcugga uaaaaagcag cagaaagaaa auacaaccga gacacuuauc     3840

gagaagcagc ugaacgacgu guucgaauau cucugccgca aacugaauca aggcauuuuu     3900

aagaacaaua agcacacccu ccccgccaaa cugcuuggag auuccaucca gaagagauuc     3960

gaggacgcga gcaucgaaga ugaguuugaa gucauuaaag agcugaucuc cgccauugcu     4020

aagaacccgc agaauuucaa aacgauccaa aaggugcugg acauuucugg cagcuccauc     4080

ccucgguuca accucaaacu gaccaagaau aacguugaug aguuuaucau uaagaaugaa     4140

ucugugacag ggcuuuucac ccaugagaaa gucaucuuuc gcaaguga                  4188


<210>  4429
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4429
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggc       60

ugggccguua cagacacgga uuauaaacug cuuaaggcua acagaaaaga ccuguggggg      120

augcggugcu uugaaaccgc cgagacagcg gaagugcgca gacuccaccg gggagcucgc      180

agacggauug agcgcagaaa gaagcggauc aaacugcuuc aggaacuguu cucccaagag      240

auugccaaga ucgaugaggg cuuuuuccag cgcaugaaag aaucuccccu cuacgccgag      300

gacaagacca uucugcagga aaauacacug uuuaacgaua aagacuucac cgauaagaca      360

uaucauaagg cuuacccuac caucaaucac cucaucaaag ccuggauuga gaacaaggug      420

aaaccggacc ccagacugcu uuaucuggcu ugucauaaua ucauuaagaa acgggggcac      480

uuucucuucg agggagauuu ugacagcgaa aaccaguucg auacguccau ccaagcccug      540

uuugaguacc uucgcgaaga cauggagcug gacauugaug cggacucuca gaaggucaaa      600

gaaauccuca aggauagcuc ccugaaaaau ucugagaaac agagcagacu gaacaagaug      660

cucggccuga aaccuuccga caagcagaaa aaggccauua ccaaucuuau cagcggcaac      720

aagaucaauu ucgcugaucu guaugacaac cccgaucuca aagacgccga gaaaaauucu      780

auuuccuuua gcaaagauga cuucgaugcu cugucugacg accuugccuc cauccugggg      840

gauagcuucg aacuccugcu gaaggcgaaa gccguguaca acugcucugu ucucuccaag      900

gugauuggag acgagcaaua ccugagcuuu gcuaagguca aaaucuauga aaagcauaaa      960

acagaucuua ccaagcugaa aaaugugauu aagaagcacu ucccuaaaga cuacaagaaa     1020

guuuuuggcu auaacaagaa cgagaaaaau aacaauaacu acuccgccua uguggggguc     1080

uguaagauca cacacaaaaa gcucgugauc aaaaauucug cuagccagga agauuucuac     1140

aaguuucuga agaccauucu uuccgccaaa agcgagauca aggaggugaa cgacauucug     1200

acggaaaucg agacaggaac cuuccucccc aaacagauuu cuaaguccaa ugccgaaauc     1260

ccuuaucagc ugcggaagau ggagcuggaa aagauccuca gcaacgcuga gaagcauuuu     1320

ucuuuccuga aacaaaagga ugagaaaggc cuuagccacu ccgaaaagau uaucaugcug     1380

cucacauuua agauuccgua cuacaucggg cccauuaaug acaaccauaa aaagucuuuc     1440

ccugaucgcu gcugggucgu uaaaaaggag aaaagccccu ccggaaagac caccccuugg     1500

aacuuuuucg accacaucga uaaggaaaag acagcggagg aguuuauuac gagcagaacc     1560

aauuucugua cauaucugau cggcgaaucu gugcugccca aguccagccu ucucuacucu     1620

gaguauaccg ugcugaauga aaucaacaau cugcagauua ucauugacgg caaaaacauc     1680

ugcgauauua agcucaaaca gaagaucuac gaggaacugu ucaaaaagua uaagaaaauc     1740

acacaaaagc agauuuccac cuuuaucaaa cacgagggga uuuguaacaa gacggacgag     1800

gucaucauuc uuggaaucga caaagaaugc acaagcuccc ugaagucuua caucgagcuc     1860

aagaauauuu ucggcaaaca gguggaugaa aucagcacca agaacaugcu ggaggaaauu     1920

auccgguggg ccaccauuua ugacgagggg gagggaaaaa caauccugaa gaccaaaauc     1980

aaggcugaau acggcaagua cuguuccgau gagcagauua aaaagauccu uaaucucaaa     2040

uuuucugggu ggggaagacu gagccgcaag uuccuggaaa caguuacguc cgagaugccg     2100

ggcuuuagcg aaccugugaa cauuaucacc gccaugcggg agacacaaaa uaaccucaug     2160

gagcugcuuu cuuccgaguu caaauuuacc gaaaauauua agaagaucaa cagcggcuuc     2220

gaagacgccg agaaacaguu uucuuauaau gggcugguca agccccucuu ccuguccccu     2280

agcgugaaaa agaugcugug gcagacacuu aaacucguua aggagauuuc ucauaucacc     2340

caggcuccuc ccaagaaaau cuuuauugaa auggccaagg gagcggagcu ggaacccgcu     2400

agaacgaaaa cccgccugaa gauccuccaa gaucuguaca gcuccugcaa aaacgacgcc     2460

gaugccuuca gcucugagau ucaguaucuu uccggcaaga ucgaaaauga ggacaaccug     2520

cggcucagaa gcgauaaacu guaccuguau uacacacagc uugggaagug uauguauugc     2580

ggaaagccga uugagaucgg ccacguguuu gacaccucca auuacgauau cgaccauauu     2640

uacccccagu cuaaaaucaa ggaugacagc auuuccaacc gcguccucgu gugcucuagc     2700

uguaauaaaa acaaggaaga caaauauccu cugaaggcug agauccaauc caagcagcgg     2760

ggguucugga acuuccugca gagaaauaac uuuauuucuc ucgaaaaacu gaaucgccuu     2820

acacggacca gccccaucuc cgaugacgag acggccaagu ucaucgcuag acagcugguc     2880

gaaacacgcc aagccaccaa aguggcggcc aagguucucg agaaaauguu uccugagaca     2940

aagauugugu acagcaaggc ugaaaccguc ucuauguucc ggaacaaauu ugauaucauu     3000

aagugcagag agaucaauga cuuccaccau gcccacgaug cuuaucugaa cauugugguu     3060

ggcaaugugu acaacaccaa auuuacaaau aaccccugga auuucaucaa ggaaaaacgg     3120

gacaacccga agauugccga uacguauaau uacuauaagg ucuuugacua cgaugugaaa     3180

cgcaacaaua ucaccgcgug ggagaaggga aaaacaauca uuaccgugaa ggacaugcug     3240

aaaagaaaca caccuaucua cacccggcag gccgcuugua agaaaggcga acuuuucaac     3300

cagacgauua ugaagaaggg gcugggacaa cacccccuca aaaaggaggg cccucugucc     3360

aauaucagca aauauggggg auacaacaag guuucugccg cuuauuacac acucauugag     3420

uaugaagaga aaggcuccaa gaucagaagc cuggaaacca ucccccuuua ccuggucaag     3480

gauauucaga aaaauaagga cgggcucaaa ucuuaucuga ccgaccagau cggcaagaaa     3540

gagauuaaga uccugauucc uaagaucaaa aucaacuccc uucugaagau uaauggauuu     3600

cccugccaua ucacaggcaa aaugggggag gccuucgugc uccgcccggc cgugcaguuc     3660

uguugcagca acgaugaagc ucuguacuuu aagaaaauua uccgguucaa ugagauuaga     3720

aagcaacgcg aaaagauggg aaaaaccauc uccccuuacg aggaccucag cuuuagaucu     3780

uauauuaagg auaaccugug uaaaaagaca aaagcggacg aaaucgagga gaaggaguuc     3840

uacgaucuuc ugcagaaaaa gaaucucgaa aucuaugaca ugcugcugac gaagcacaaa     3900

gauaccauuu acaagaaacg gcccaacagc gccacaaucg acauucuugu caagggcaaa     3960

gaaaaguuua agucccugau cauugagaau caguucgaag ugauccucga gauccugaaa     4020

cucuuuucug cuacccgcaa cguuagcgau cugcagcaua uugggggauc caagaaugcc     4080

ggcaacgcca gaguggggaa uaaaaucucu agccuugaca acugcauucu gaucuaucaa     4140

uccauuacag gcaucuucga gaagcggauc gaucugcuca aaauuuga                  4188


<210>  4430
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4430
augagcuuca agaaaauccu gggccucgac gugggggucg ccuccauugg augggcuguu       60

gugagagaga acgaagagaa ucccaacuuu aauaccauca caaagcuggg cgugcggguc      120

gugccuauua cgucugauga aaaacuuaac uucgagaagg ggaaaagcau caccuccaau      180

gccgaacgca cacuggcgag aucuagccgg cgcaaccucc agagauuucg gcugcgcaga      240

aagaaucuua uugcuauccu gcuccaaaac cagaucauua ccccgacaac accccugaca      300

gagaagggaa aauucaccac guuugagacc cugaagaucc gggccaaagc cacaaaggag      360

aaaauugaac ucgaggagcu ggcucgcguu cuucugcaca ucaauaagaa gagaggcuac      420

aaauccucuc ggaaggugaa aaacagcgag gacgggaagg ccauugaugg aauggaagcu      480

gccaaaaucc ucuaugagaa uaagcugacc ccuggccagu acacauauca gcuucuggaa      540

aaaggcucca acaagauucc cgacuucuac ugcagcgauc uccaaaucga gugggaaguc      600

aucuggaauu uucagaagca guucuauccu aaccugcuga ccgaggacuu uaaaaaggag      660

cucaaaggga uuggacagcg cgcgacggcc gcucuguucc uuaagaaaua cggcaucuau      720

acagcccuga auaaggggaa gagagaagag aaaaagcucc aggcuuacaa auggcggucu      780

aaguccguga gcgagaaaau ucccguugaa gaacuggccu acgugaucgc ggagauuaac      840

ucucauauca ccaacuccag cggauaucuu ggcacaaucu cugaucgcuc caaggagcug      900

uacuuuaauc agcagaccau uggggaaaac cucuaugccc aggucgcuaa gaauacccac      960

gccaaacuga agaaccaagu guuccugaga caggacuaca gcgcugaguu ugaacagauc     1020

ugggagacac aagccaaauu ccauccucag cucacgccgg aacugaagaa aaagauucgg     1080

gauuccauca uuuucuauca gagaaaacuu cgcucucaga aacggcugau cagcaucugu     1140

gaguuugaga auagagaaau ugagaucauu aagaacggca aaaagaaaaa gaaaaagauc     1200

ggacucaagg ccgcucccaa auccagcccu cuguuccaag aauuuaagau uuggcaagug     1260

cuuaauaaca ucgucauuaa acaggagggc aaggaccgcc ugcucaccug ggaggaaaaa     1320

gagcugcugu ucacagaacu caauaucaag gggaacaucu cuggacagaa ggcgcuggcc     1380

cuucugaaac ucucccccaa ggauaccaag auuaauguua caauccugga aggcaaccac     1440

acccaggaac aacuguaccg gguguaucuu cgcauucucg ggauggaggg auacgacgug     1500

uuugaucugc ugaagauccc ugagaaucag gacgaaauua aacccgcuga cagcaagauc     1560

ucugccgccg agaucaagga aacggucaaa agcguguuca agacaugcuc cauuucuccg     1620

agcauccucg auuuugaccc ucugcuuccc aacccugagu ucgaaaauca guccagcuac     1680

caacuguggc aucuccugua uucuuacgag ggcgauucca gccccucugg gaacgagaaa     1740

cugcuugaac uccugaagaa caaauuugga uucaagaagg agcacgcucu gcuccuggcc     1800

uccauuagcu auaccuacau cuauggcaau cuuuccucua aagcgaugag aaagauuuac     1860

cccuauagca aagaaaacac cuacuccgag gcuuguaugc uggccggcua ccggcacucu     1920

aagaaugccc ucccuagaga ggaacugguu aaauaucagc ugaaggaccg ccuugagauc     1980

cucccgaaaa acagccugcg gaaucccgug gucgaaaagg ugcugaacca gcucaucaau     2040

cugguuaacu cccuuaugga ugaguacggg gcuuuugacg aaauuagacu ggagauggcc     2100

cgcgagcuca agaaaaaugc uaaggaacgg gagaaaugga gcaagaacau cgccgaugcg     2160

acaucuagaa aucagaaaca ugccgaaauu cugaagaagg acuucaaaau cuccaacccu     2220

agcaagaaug aucugauucg guaucgccuu uaccucgagc ugaaagcuaa cggauauaau     2280

gaccuguaca ccaacacaua uaucgauauc aacuaccucu uuacgcacag auacgacauu     2340

gaccauauca uuccccaauc uaaggugcug gaugacuccu ucagcaauaa aguccuugug     2400

ccucggcagg ccaaccugga aaagucuaau cucaccgcuu ucgauuauau cagccaccag     2460

ggcaaggaga aagaggaaca cuaccugaac gucauuacag agcuguauaa gagagggucc     2520

aucagcgaaa ccaaauacaa gaaucuucag aaaaagggaa acgagauugg cgaaggguuu     2580

aucaaucgcg accugaagaa cacacaauau aucgugcgga aagccagaga gauucucucu     2640

cugaucaccc ccaauguugu gacgaccaca ggcgagauua ccgcgaagcu ccgcaaagau     2700

uggaaccuga ucaauuccau gaaggaacuu aacauggaca aauucagaaa ugcccggcug     2760

acagagcucg ucgaaaacag ccgcggagag aagaaggaaa uuaucaucga uuggaauaaa     2820

agaaacgacc agcggcauca cgcuauugau gcccuggugg uugcuuuuac cacgcgcaac     2880

caugugcagu accugaauua uaugaacgcc agagaggacg agucccacaa gcaacauucu     2940

cacauccuua gcauugagaa aaagaucaaa uacgauaagu uccacgaaaa uggcucccgg     3000

cagucucgcu uuauuuaccc gacaagcaac uucagagaag aggccaaaaa gaagcugaaa     3060

gagauccucg ucuccuauaa gaccaaaaau aaggugguga caguuaacau caauaaaccu     3120

aagaagaaag ggggauacuc ucccaagcau gaacugaccc cucggggccc ccuccacaaa     3180

gagaccauuu augggaugau ccgcauucug cauacaaagg aaaucaaagu cagcuccaaa     3240

cuuaacuuug agacgguggu gcggauucag aauccuagau acagaaacgc ucugcucgaa     3300

cgccugaagg acaauuucaa cgaucccaag aaggcguuua gcggaaccaa uucucugacc     3360

aaaaacccgg ucuuccuuac cgccaauucc acagagaccg ugccugagaa gguuacgauc     3420

cagugguuug aaaaagugua uacaauucgg aagcccauca accccaccaa uuucaagaac     3480

gagaaaaaca ucgaaaaggu ccuggacccu accauuagaa cacuccugaa aaagcgccuc     3540

caagaguaca auggcaacgc uaaagaagcc uuuagcgacc uggagaagaa uccgaucugg     3600

cuuaaccagg agaaagggau uugcaucaag accgugacaa uuacgggcau cucuaauguu     3660

gaaucccugc acaccaagaa agaucacuuc ggaaaggagc ugcucgacau caacggcaau     3720

cccauuccug uggauuucgu cagcacaggg aacaaucauc acguggccau cuaugaagac     3780

ucugagggaa accugcagga aaaaguugug uccuuuuacg aggcugucga gcgggugaau     3840

cagggccuuc ccgugauuga uacccuguac aaccucgacc ugggguggaa guuccucuuu     3900

acaaugaaac aaaaugaaau guuccuguuu ccugccgaug acuucgaucc caacgacauc     3960

gauauuuaug acgagaagaa ucuuauccug aucagcaagc aucuguuuag aguccagaaa     4020

cucuccagca aggauuacug uuuccgccac caucuggaaa ccucuauuaa cuuuaacgac     4080

ccugcgcuua aaggaaucac guggcggaga gagagcccca auggccugcg caaccucguu     4140

aaggugcggc ugaaucaccu cgggaaaauu gugcacaucg gcgaguga                  4188


<210>  4431
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4431
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggc       60

ugggccguua cagacacgga uuauaaacug cuuaaggcua accacaaaga ccuguggggg      120

augagaugcu uugaaaccgc cgagacagcg gaaaagcggc gccucuucag aggagcucgg      180

cgcagaauug agcggcgcag aaagcggauc aaacugcuuc aggaacuguu uucccaagag      240

auugccaaga ucgaugagga cuucuuucag cgcauggaag agucucccuu cuacgccgaa      300

gauaaaacca uucuccagga gaauacacug uuuaacgaca gagauuucac cgacaagaca      360

uaucauaagg cuuacccuac caucaaucac cugaucaagg ccuggauuga gaacaaggug      420

aaaccggauc cccggcuccu guaucuugcu ugucauaaua ucauuaagaa acgcggccac      480

uuucuguucg aaggggacuu ugauagcgag aaccaguucg acacguccgu gcaagcccuc      540

uuugaauacc ugagagagga cauggaacuu gauaucgacg cggauucuaa gaaaauuaag      600

gagauccuga aagacagcgc ccucaaaaau uccgauaagc agaaccggcu gaauaaacug      660

cucggacuga agucuagcga caaacagaag aaagcucuua ccaaccugau uuccggcaau      720

aaggccaacu ucgcugaucu cuaugacaau ccugaucuga aggacgccga gaaaaacagc      780

aucucuuucu ccaaggauga cuuugacgcg cuuagcgaug aacuggccuc uguccucggc      840

gacuccuucg agcugcugcu ccgcgcuaaa gccguguaca auugcagcgu ucugucuaag      900

gugaucgggg augaacagua ccuuuccuuu gcuaaaguca agauuuauga gaagcauaaa      960

aacgaccuga caaagcucaa agaagugguu aagaaauacu uccccaucga guauagaaac     1020

auuuuuggau acaagcaaag cgagaauaac aauuguaacu auucccggua cgugggcguc     1080

ugcaagucug ggagcaaaaa gaaagugauc aauaagaccg ccucccagga agauuucuau     1140

aaauuucuga aggacauucu ucugaaaaga agcgaugugc cugaggccaa cggaauccuc     1200

acagaaaucg agaccggcac guuccugccc aagcagauuu cuaagaauaa cgcugaaauc     1260

ccuuaccagc ugcgcaaaau ugagcucgag aagauccuga caaaugcgga aaaauuuuuc     1320

uccuuucuua agcaaaaaga cgagaacggg cugagccacu cugaaaagau uaucaugcuc     1380

cugaccuuca agaucccgua cuauauugga cccaucaaua caagccacaa acaguacuuu     1440

ccugaucggu ccugggucau uaagaaagag aacgcucccc aggagaagau caccccuugg     1500

aauuucuucg accauauuga uaaagaaaag accgccgagg ccuuuaucac aucuagaacg     1560

aacuucugua ccuaucugau uggcgaaagc guucuuccca aaaauucccu ccuguacagc     1620

gaguauacag ugcugaacga aaucaacaau cuccaaauca uuaucgacgg caagaacauu     1680

ugcgauguga agcugaaaca gaagaucuac gaggagcuuu uuaaaaagua uaagaaaguc     1740

acccagaagc agauuucuac auucaucaaa cacgaaggga ucuguaauaa gaccgacgag     1800

gugauuaucc ugggaauuga caaagaaugc acguccagcc ucaagucuua caucgagcug     1860

aaaaacauuu uuggcaagca aguugaugaa aucuccacaa agaauaugcu ggaggacauc     1920

auucgcuggg ccaccaucua cgaugagggg gaaggaaaaa ccauucuuaa gacaaaaauc     1980

aaggcugagu augaaaaaua cuauagcgac gagcagauua agaagauccu caaccugaaa     2040

cuguccggcu gggggcggcu cucuagaaag uuccuggaaa gcaucaccuc cgagauuccg     2100

ggauuuaucg agccugugaa uauuaucaca gccaugcgcg aaacgcagaa caaucuuaug     2160

gagcugcucu cuagcaaauu caaguuuaac gaaaauauua aaaagaucaa cuccggcuuc     2220

gaggauaccg acaagcgguu uagcuacaau ucucugguca aaccccuguu ccuuuccccu     2280

agcgugaaga aaaugcucug gcagacacug aagcugguua aagagauuuc ucauaucacc     2340

caagcgccuc ccaagaagau cuuuauugaa auggcuaaag gcgccgagcu cgaacccgcc     2400

agaacaaaga cccgccugaa aauccuucag gaucuguaua acuccuguaa gaaugacgcu     2460

gaugccuuca gcucugagau ucaguaccuc cuggggaaaa ucgaaaacga ggacaaccug     2520

cggcuuagaa gcgauaagcu cuaucuguac uacacgcagc ugggaaagug cauguauugu     2580

ggcaaaccga uugagaucgg gcacguguuc gacaccucca aguacgauau cgaccacauu     2640

uauccccaaa gcaaaaucaa ggacgauucu auuaauaacc gcguccucgu gugcuccagc     2700

ugcaauaaaa acaaggaaga caaauacccu cugaagcccg agauccagcu uaagcagaaa     2760

aaguuuugga auuuccugca gcggaacaau uuuauuucca ucgaaaaauu caacagacuc     2820

acacggacca caccuaucuc ugaugacgag accgcuaagu uuauugcccg ccaacugguc     2880

gaaacgagac aggcgacaaa aguggccgcu aagguucugg agaagaucuu ccccgagacc     2940

aauauugugu auagcaaagc cgaaacaguc uccauguuuc ggaacaaguu cgauaucauu     3000

aaauguagag agaucaauga cuuucaucac gcucaugaug ccuaccuuaa cauugugguu     3060

ggaaaugugu auaacaccaa guucaccaau aacccgugga acuuuaucaa agaaaagcgc     3120

gacaauccua agaucggcga uacauacaac uacuauaaag ucuucgacua cgaugugaag     3180

cggaauaaca uuacggcgug ggagaaagaa aagaccauca uuacagugaa aaccaugcug     3240

aagagaaaua cacccaucua uacccgccag gccgcuugca agaaagggga acucuucaac     3300

caaacgauua ugaagaaagg ccugggacag cacccucuca agaaagagug ucccuuuucu     3360

aauaucagca aguacggcgg guauaacaaa guuuccgccg cuuacuauac auucaucgag     3420

uacgaggaaa agggaaauaa gauuagaucu cuggagacca ucccucuuua ccuggagaaa     3480

gacauucaaa agagccagga cggccucaaa ggguaucuga ccgaucagcu gggaaagaaa     3540

gaaaucaaga uucuuguccc caagaucaaa aucaacuccc ugcucaagau uaauggcuuu     3600

ccgugccaua ucacagggaa aaccggcgcc agcuucgugc ugcggccugc cgugcaguuu     3660

uguugcucua acgacgaggu ccucuacuuc aagaaaauua uccgcuuuaa ugaaauuaga     3720

ucccagcggg agaagauggg aaagacaauc agccccuaug aagaucuguc uuuccgcucc     3780

uacauuaaag agaaccuuug uaagaaaacg aagaaugacg agaucggcga aaaagaguuu     3840

uauggguucc ugcagaagaa gaaccucgaa aucuacgaua ugcugcugau uaaacacaag     3900

gacaccaucu auagcaaaag acccaauucu acaacccuug auaugcugau uaagggacgg     3960

uacgaguuua ucaaccucaa accugaagac caaauucgcg ugaugcugga gauccucaag     4020

cuguucugca caaccagaga gguuaucgau cuuggccuga uuaaagggaa guccgaagcu     4080

ggagugacga cccugggcaa gaaaaucagc aaccucgacu ccuguauucu gaucuaccag     4140

agcauuacag ggaucuuuga gaagcggauc gaucuucuga aaauuuga                  4188


<210>  4432
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4432
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggc       60

ugggccguua cagacacgga uuauaaacug cuuaaggcua acagaaaaga ccuguggggg      120

augcggugcu uugaaaccgc cgagacagcg gaagugcgca gacuccaccg gggagcucgc      180

agacggauug agcgcagaaa gaagcggauc aaacugcuuc aggaacuguu cucccaagag      240

auugccaaga ccgaugaggg cuuuuuccag cgcaugaaag aaucucccuu uuacgccgag      300

gacaagacaa uccuccagga aaaugcucug uucaacgaua gagacuuuac cgauaaaaca      360

uaucauaagg ccuacccuac cauuaaucac cugaucaagg cuuggaucga gaacaaagug      420

aagccggacc cccggcuccu guaucuugcc ugucauaaua uuaucaaaaa gcgcgggcac      480

uuccuguuug agggagauuu cgacagcgaa aaccaguuug auacguccau ucaagcgcug      540

uucgaguacc ugagagaaga cauggagguc gacaucgaug ccgacucuca gaaagugaag      600

gaaauucuua aagauagcuc ccugaagaau agcgagaagc agagccggcu caacaaaaug      660

cugggccuga agccuuccga caaacagaag aaagcuauca ccaaucucau uagcggcaac      720

aagaucaauu uugccgaucu guaugacaac cccgaucuua aggacgcuga gaaaaauucu      780

aucuccuuca gcaaggauga cuucgaugcc cugucugacg accucgcguc cauucugggg      840

gauagcuuug aacuucugcu caaagccaag gcuguuuaca acugcucugu gcuguccaaa      900

gucaucggag acgagcaaua ccugagcuuc gccaagguga agauuuauga aaaacauaag      960

acagaucuca ccaaacugaa gaauguuauc aaaaagcacu uuccuaacga cuacaagaaa     1020

guguucggcu auaacaagaa ugagaaaaac aauaacaauu acuccgcuua ugucggggug     1080

uguaagacaa aaucuaagaa acuugugauu aacaauaccg ucaaccagga agauuuuuac     1140

aaguuccuga agcccauccu ccuggccaaa agcgagauca aggagguuaa ugacauucuu     1200

acggaaaucg agacaggaac cuuucugccu aaacagauuu ccaagagcaa cgccgaaauc     1260

ccguaucagc uccgcaaaau ggagcuggaa aagauucugu cuaaugcuga gaagcacuuc     1320

uccuuucuca aacaaaagga ugagaaaggc cugagccauu cugaaaagau caucaugcuu     1380

cugacauuca aaauucccua cuacaucggg ccuauuaacg acaaucacaa gaagucuuuu     1440

cccgauagau gcugggugau caaaaaggag aaauccccuu cuggaaagac cacccccugg     1500

aacuucuuug accauauuga uaaagacaag acagcggaag ccuucaucac gagccggacc     1560

aauuucugua cauaucucgu gggcgagucc guccugccga agaacagccu gcuuuacucu     1620

gaguauaccg ugcucaacga aauuaauaac cugcagauca ucauugaugg caaaaauauc     1680

ugcgacauua agcugaaaca gaagaucuac gaggaacucu uuaaaaagua uaaaaagauu     1740

acacaaaagc agaucuccac cuucaucaaa cacgagggga uuuguaacaa gacggacgaa     1800

guuaucauuc ugggaaucga uaaagagugc acaagcucuc uuaaguccua cauugagcug     1860

aaaaauaucu uuggcaaaca gguggacgaa aucagcacca agaacaugcu cgaggaaauu     1920

aucagauggg cuaccauuua ugaugagggg gaaggaaaga caauccugaa gaccaaaauu     1980

aaggccgagu acggcaaaua cuguuccgac gagcagauca agaagauccu gaaucuuaaa     2040

uucucugggu ggggacgccu cagccggaag uuucuggaaa cagucacguc cgagaugccu     2100

ggcuucucug aacccgugaa cauuaucacc gccaugagag agacacaaaa uaaccugaug     2160

gaacuccuga gcuccgaguu uaaauucacc gagaauauua agaaaaucaa cagcggcuuu     2220

gaagauguug agaagcaguu cucuuaugac ucccuuguga agccucuguu ucucagcccc     2280

ucugucaaaa agaugcugug gcagacacug aaacuuguga aggaaauuuc ccacaucacc     2340

caggcucccc cuaaaaagau uuucaucgag auggccaagg gggcggagcu cgauccgguc     2400

cgcacgaaaa cccggcugaa gauccugcaa gaccucuaca auaacugcaa aaaugaugcu     2460

gacgccuuua gcucugaaau uaaggaucug agcggaaaaa ucgagaacga agacaaccuu     2520

agacugcgcu ccgauaagcu cuaucuguac uauacacagc ugggcaaaug uauguacugc     2580

gggaagccca uugagaucgg acauguguuc gacaccagca auuaugacau ugaucacauc     2640

uacccucagu cuaagaucaa agacgauucc auuagcaacc ggguucuugu gugcuccucu     2700

uguaauaaga acaaagaaga caaguacccc cucaaagccg agauccagag caagcaaaga     2760

ggcuucugga auuuucugca gcgcaacaau uucauuuccc uggagaagcu caaccggcug     2820

acaagaaccu cuccuaucag cgaugacgaa acggcuaaau uuauugcccg ccagcuuguc     2880

gagacacggc aggcuaccaa gguggccgcg aaaguucugg aaaagauguu ccccgagaca     2940

aaaaucgugu auuccaaggc cgaaaccguc ucuauguuua gaaauaaguu cgauaucauu     3000

aaaugccggg agaucaacga cuuucaucac gcucaugaug ccuaccucaa uauuguggug     3060

gggaacguuu auaauaccaa guucacaaac aauccgugga acuuuaucaa agagaagcgc     3120

gacaacccua aaauugcuga uacguacaau uauuacaagg ucuucgacua ugacgugaag     3180

agaaacaaua ucaccgccug ggaaaaaggc aagacaauua ucaccgugaa agauaugcug     3240

aagcggaaca cacccaucua caccagacaa gcggccugua aaaagggaga gcuguuuaau     3300

cagacgauua ugaaaaaggg ccuugggcag cacccucuga agaaagaagg accccucagc     3360

aacaucucca aguacggcgg guauaauaaa gucagcgcug ccuacuauac acugauugag     3420

uacgaagaga agggaucuaa aauccgcucc cucgagacca uuccucugua ucuugugaag     3480

gacauccaaa agaacaaaga uggccucaag agcuaccuca ccgaccagau cgggaaaaag     3540

gaaauuaaaa uccugauucc caagaucaag auuaauucuc ugcuuaaaau caacggcuuc     3600

ccgugccaca ucacaggaaa gaugggcgag gcuuucguuc ugcggccugc cgugcaguuu     3660

uguugcucca augaugaagc ccucuauuuc aaaaagauua ucagauuuaa cgagauucgc     3720

aaacagagag agaagauggg gaagaccauc agccccuacg aagaccuguc uuuccggucc     3780

uacauuaaag auaaucucug uaagaaaaca aaggcugacg agaucgaaga gaaagaauuu     3840

uaugaucugc uucaaaagaa aaaccuggag auuuacgaca ugcuccugac gaagcauaag     3900

gauaccaucu auaaaaagcg ccccaauagc gcgacaaucg acauucuggu caaaggaaag     3960

gagaaauuca agucccuuau cauugaaaac caguuugagg ugauccugga aauucucaag     4020

cuguucagcg ccaccagaaa uguuucugau cuccagcaca ucggcgggag caaaaacgcu     4080

ggaaacgccc gggugggcaa uaagaucucc ucucuggaca acugcauucu uaucuaccag     4140

agcauuacag ggaucuuuga gaaacgcauu gaccugcuga agaucuga                  4188


<210>  4433
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4433
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggc       60

ugggccguua cagacacgga uuauaaacug cuuaaggcua acagaaaaga ccuguggggg      120

augcggugcu uugaaaccgc cgagacagcg gaagugcgca gacuccaccg gggagcucgc      180

agacggauug agcgcagaaa gaagcggauc aaacugcuuc aggaacuguu cucccaagag      240

auugccaaga ucgaugaggg cuuuuuccag cgcaugaaag aaucucccuu uuacgccgag      300

gacaagacca uucuccagga aaaugcucug uucaacgaua aagacuuuac agauaagacc      360

uaucauaagg ccuacccuac aaucaaucac cugaucaagg cuuggauuga gaacaaggug      420

aaaccggacc ccagacuccu guaucuugcc ugucauaaua ucauuaagaa acgggggcac      480

uuccuguuug agggagauuu cgacagcgaa aaccaguuug auaccuccau ccaagcccuc      540

uucgaguacc ugcgcgaaga cauggagguc gacauugaug ccgacucuca gaaggugaaa      600

gaaauccuua aggauagcuc ccugaaaaau ucugagaaac agagcagacu caacaagauu      660

cugggccuga aaccuuccga caagcagaaa aaggcuauca cgaaucucau cagcggcaac      720

aagauuaauu uugccgaucu guaugacaac cccgaucuua aagacgcuga gaaaaauucu      780

aucuccuuca gcaaagauga cuucgaugcc cugucugacg accucgcguc cauucugggg      840

gauagcuuug aacuucugcu caaggccaaa gcuguuuaca acugcucugu gcuguccaag      900

gucaucggag acgagcaaua ccugagcuuc gccaagguga aaauuuauga aaagcauaaa      960

accgaucuca caaagcugaa agcuguuauc aagaagcacu uuccuaauaa cuacaaaaag     1020

guguucggcu auaaccagga caaugagucc aaaaacaauu acucugggua ugucggagug     1080

uguaagacca aaaacaagaa acuugugauc aauaacagcg ucaaucagga agauuuuuac     1140

aaguuccuga agcccauucu cuccgccaaa agcgagauca aggagguuaa cgacauucug     1200

gccgaaaucg agacaggcaa uuuucuuccu aaacagauuu cuaaguccaa cgcugaaauc     1260

ccguaucaac ugcggaaaau ggagcucgaa aagauccuga gcaaugcgga gaagcacuuc     1320

ucuuuucuga aacagaagga ugagaaaggg cucagccauu ccgaaaagau uaucaugcug     1380

cuuaccuuca aaauucccua cuacaucgga ccuauuaacg acaaucacaa gaagucuuuu     1440

cccgaucgcu gcuggguggu gaaaaaggag aaaagcccuu ccggcaagac gacacccugg     1500

aacuucuuug accauaucga uaaagaaaag accgccgagg aguucauuac aagcagaacc     1560

aauuucugua ccuaucugau cggcgaaucu guccucccga agaacucccu gcuguacagc     1620

gaguauacag ugcuuaacga aaucaauaac cuccagauua ucauugacgg gaaaaauauc     1680

ugcgauacga agcugaaaca aaagauuuac gaggaccugu uuaaaaagua uaaaaagauc     1740

acccagaagc agaucucuac auucauuaaa cacgaaggaa ucuguaacaa gaccgacgag     1800

guuauuaucc ucggcauuga uaaagagugc acauccagcc ugaaguccua caucgaacuu     1860

aaaaauaucu uugggaaaca gguggacgag auuucuacca agaacaugcu ggaagagauc     1920

auucgguggg cuacgaucua ugaugaagga gagggcaaaa caauucucaa gaccaaaauc     1980

aaggccgagu acgggaaaua cuguagcgac gaacaaauca agaagauucu gaaucugaaa     2040

uucuccggau ggggcagacu uucucgcaag uuucucgaga ccgucacaag cgaaaugccu     2100

ggcuucuccg agcccgugaa caucauuacc gccaugcggg aaacacagaa uaaccugaug     2160

gagcugcuca gcucugaguu uaaauucacg gaaaauauca agaaaauuaa cuccggguuu     2220

gaggaugcug aaaagcaguu cagcuaugac ggacugguua agccucuuuu ucugucuccc     2280

uccgugaaaa agaugcucug gcagacccug aaacugguca aggagaucag ccacauuaca     2340

caagcgcccc cuaaaaagau cuucaucgag auggcgaagg gcgcugaacu ugagccggcc     2400

agaaccaaaa cacgccucaa gauucugcag gaucuguacu cuagcugcaa aaaugacgcc     2460

gaugcuuuuu ccagcgaaau ccaguaucuc ucugggaaga uugagaacga agacaaucug     2520

cggcuuagau ccgauaaacu guaccucuau uacacccagc ugggaaagug uauguauugc     2580

ggcaaaccca ucgagauugg gcauguguuc gacacgagca acuacgauau cgaccacauc     2640

uacccucaau ccaagauuaa ggacgauucu aucagcaacc gcguccuggu gugcuccucu     2700

uguaauaaaa acaaggagga caaauauccc cuuaagagcg aaauucaguc caaacagcgg     2760

ggcuucugga auuuucucca gagaaacaau uucaucucuc uggagaagcu gaaccgccuc     2820

acccggacaa ccccuauuag cgaugacgaa acagccaagu uuaucgcuag acaacugguu     2880

gagacccgcc aggccacgaa aguggcggcc aagguccuug aaaagauguu ccccgagaca     2940

aagaucgugu acuccaaagc ugagaccguu agcauguuuc ggaauaaguu cgauauuauc     3000

aagugcagag aaauuaacga cuuucaucac gcccaugaug cuuaucugaa uaucgugguc     3060

ggaaacgugu acaauacaaa auucaccaac aauccgugga acuuuauuaa ggagaaacgg     3120

gacaauccua agaucgccga uaccuauaac uacuauaaag uguucgacua cgauguuaag     3180

cgcaacaaua uuacagcgug ggaaaagggc aaaacgauca ucaccgucaa ggacaugcuc     3240

aaaagaaaca cacccauuua cacccggcag gccgcuugua agaaagggga gcuguuuaau     3300

caaacaauca ugaagaaagg acugggccag cacccucuua agaaggaagg gcccuucucu     3360

aacauuucca aauauggagg cuacaauaag gugagcgccg cuuauuacac ccugaucgag     3420

uaugaggaaa aagugaacaa gauuagaucu cucgagacga ucccucugua ccucgucaaa     3480

gacauccaga aggaucagga cgugcugaag uccuaucuua cagaucugcu cgggaaaaag     3540

gaaauuaaaa uccugguucc caagauuaaa aucaauagcc ugcuuaagau uaacggcuuc     3600

ccgugccaca ucaccggaaa gaccggcgac ucuuuugugc ugcgcccugc cguccaauuc     3660

uguugcucca augaugaggu gcucuacuuu aaaaagauca uucgguucaa cgagaucaga     3720

agccagcgcg aaaagauggg gaagacaauu ucccccuacg aggaccugag cuuuagaucu     3780

uauaucaaag aaaaucucug uaagaagacc aaagccgaug agauuggaga aaaggaguuc     3840

uacgaccugc uucagaaaaa gaaccuggag aucuaugaua ugcuccugac aaaacauaag     3900

gacacgauuu acaauaaacg gcccaacagc accacacugg gcaugcuuac caaggggcgc     3960

uaugaauuua ucaaucugaa gccuaaagau cagaucauug uuaugcucga gauccugaaa     4020

cucuucugca caaccagaga agcuauugac cugggacuua ucaagggcaa gccggcggcc     4080

gggguggcua cgcugggcaa aaagauuucc aaccuggaua auuguauccu caucuaccaa     4140

ucuauuaccg gaaucuuuga gaagcggauu gaccugcuua aagucuga                  4188


<210>  4434
<211>  4188
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4434
augaagaaag agaucaagga cuacuuccug ggccucgaug uggggaccgg aagcgucggc       60

ugggccguua cagacacgga uuauaaacug cuuaaggcua acagaaaaga ccuguggggg      120

augcggugcu uugaaaccgc cgagacagcg gaagugcgca gacuccaccg gggagcucgc      180

agacggauug agcgcagaaa gaagcggauc aaacugcuuc aggaacuguu cucccaagag      240

auugccaaga ucgaugaggg cuuuuuccag cgcaugaaag aaucuccccu cuacgccgag      300

gacaagacca uucugcagga aaauacacug uuuaacgaua aagacuucac cgauaagaca      360

uaucauaagg cuuacccuac caucaaucac cucaucaaag ccuggauuga gaacaaggug      420

aaaccggacc ccagacugcu uuaucuggcu ugucauaaua ucauuaagaa acgggggcac      480

uuucucuucg agggagauuu ugacagcgaa aaccaguucg auacguccau ccaagcccug      540

uuugaguacc uucgcgaaga cauggagguc gacauugaug cggacucuca gaaggugaaa      600

gaaauccuga aggauagcuc ccucaaaaau ucugagaaac agagcagacu gaacaagaug      660

cugggccuca aaccuuccga caagcagaaa aaggccauua ccaaucugau cagcggcaac      720

aagaucaauu ucgcugaucu uuaugacaac cccgaucuga aagacgccga gaaaaauucu      780

auuuccuuua gcaaagauga cuucgaugcu cucucugacg accuggccuc cauccuuggg      840

gauagcuucg aacugcuccu gaaggcgaaa gccguuuaca acugcucugu gcuguccaag      900

gucauuggag acgagcaaua ccucagcuuu gcuaagguga aaaucuauga aaagcauaaa      960

acagaucuga ccaaacuuaa aaauguuauu aagaagcacu ucccuaaaga cuacaagaaa     1020

guguuuggcu auaacaagaa cgagaaaaau aacaauaacu acuccgggua ugucggagug     1080

uguaagacaa aaucuaagaa gcugaucauc aauaacagcg ugaaucagga agauuucuac     1140

aaauuucuca agaccauucu guccgccaaa agcgagauca aggaggucaa cgacauucuu     1200

acggaaaucg agacaggcac cuuccugccc aaacagauuu cuaaguccaa ugcugaaauc     1260

ccuuaucagc uccggaagau ggagcuggaa aagauccuga gcaacgccga gaagcacuuu     1320

ucuuuccuca aacaaaagga ugagaaaggg cugagccauu ccgaaaagau uaucaugcuu     1380

cugacauuua agauuccgua cuacaucgga cccauuaaug acaaccacaa aaagucuuuc     1440

ccugaucgcu gcuggguugu gaaaaaggag aaaagccccu ccggcaagac caccccuugg     1500

aacuuuuucg accauaucga uaaggaaaag acagccgagg aguuuauuac gagcagaacc     1560

aauuucugua cauaucucau cggcgaaucu gugcugccca aaaauucccu gcuuuacagc     1620

gaguauaccg uccucaacga aaucaacaau cugcagauua ucauugacgg gaaaaacauc     1680

ugcgauacaa agcugaaaca gaagauuuac gaggaacucu ucaaaaagua uaagaaaauc     1740

acccaaaagc agaucucuac guuuauuaaa cacgagggaa ucuguaauaa gacagacgag     1800

gugauuaucc ugggcauuga caaagaaugc accuccagcc uuaaguccua caucgagcug     1860

aagaacaucu ucgggaaaca gguugaugaa auuucuacca agaauaugcu cgaggaaauc     1920

auucgguggg cuacaaucua ugacgaggga gagggcaaaa ccauucugaa gacaaaaauc     1980

aaggcggaau acgggaagua cuguagcgau gagcagauca aaaagauucu gaaccuuaaa     2040

uuuuccggau ggggcagacu cucucgcaag uuccuggaaa cggugaccag cgagaugccg     2100

ggcuuuuccg aaccugucaa uaucauuaca gccaugcggg agacccaaaa caaucugaug     2160

gagcuccuga gcucugaguu caaauuuaca gaaaacauca agaagauuaa uuccggguuc     2220

gaagacgugg agaaacaguu uagcuaugau ggacuuguua agccccuguu ccucucuccu     2280

uccgugaaaa agaugcugug gcagacccug aaacuuguca aggagaucag ccacauuacg     2340

caggcuccuc ccaagaaaau cuuuaucgaa auggccaagg gcgccgagcu cgaccccgug     2400

agaaccaaaa cacgccugaa gauucugcaa gaucucuaca acaauugcaa aaacgacgcu     2460

gaugccuucu cuagcgaaau caaggaccug uccgggaaaa uugagaauga agauaaccuu     2520

cggcugagaa gcgacaagcu cuaucuguac uauacccagc ugggaaagug uauguacugc     2580

ggcaaaccga ucgagauugg gcaugucuuu gauacaucua acuaugacau cgaccacauc     2640

uacccccagu ccaagauuaa agaugacagc aucuccaauc gcgugcuugu uugcucuagc     2700

uguaacaaga auaaagagga uaaguacccu cucaaggcgg aaauucaguc caaacaacgg     2760

ggcuucugga acuuccugca gagaaauaac uuuaucucuc uggagaaguu caaucgccuc     2820

acccgggcua cgcccauuag cgacgaugaa acagccaaau uuaucgccag acagcuggug     2880

gagacccgcc aggcuacaaa ggucgccgcu cgggugcuug aaaagauguu cccugagacc     2940

aagaucguuu auuccaaggc cgagaccgug ucuauguuua gaaacaaauu cgacauuguc     3000

aagugccggg aaaucaauga uuuucaucac gcgcaugacg ccuaccugaa cauuguggug     3060

ggaaauguuu auaacacaaa auucacgaau aaccucugga auuuuaucaa ggagaaacgc     3120

gauaacccca agauugcuga caccuacaac uauuacaagg ucuucgauua ugacgugaaa     3180

agaaauaaca ucacagccug ggaaaagggc aaaaccauua ucacagugaa ggacaugcug     3240

aaacggaaua ccccgaucua cacgagacaa gcugccugua agaaaggggg acuguuugau     3300

cagacaauua ugaagaaggg ccuugggcag cacccucuga aaaaggaggg acccuucagc     3360

aacaucucca aauacggcgg guauaauaag gucagcgcgg ccuacuauac ccucauugaa     3420

uacgaggaga aaggcaacaa gauccgcucu cuggaaacca uuccucucua ucuggugaag     3480

gacauccaaa aagaucagga cguucuuaag uccuaccuga cagaucuccu gggaaagaag     3540

gaguucaaaa uccuggugcc caagauuaag aucaauagcc uucugaaaau uaacggcuuu     3600

ccuugccaca ucaccgggaa gacaaaugac ucuuuccucc ugcggcccgc uguccaguuu     3660

uguugcucca acgaugaagu gcucuauuuc aaaaagauua ucagauuuag cgagauccgc     3720

ucucagagag agaaaauugg aaagacgauc uccccguacg aagaccugag cuuccggucc     3780

uacauuaagg agaaucuuug uaaaaagacc aaaaacgaug aaaucggcga gaaggaauuu     3840

uaugaccugc uccagaaaaa gaaucuggag auuuacgaua ugcugcuuac aaaacauaag     3900

gacaccaucu auaagaaacg cccuaacagc gccacaauug auauccuggu uaaggggaaa     3960

gagaaguuca aaucucucau cauugaaaau caauuugagg ugauccugga aauucucaag     4020

cuguucagcg cuaccagaaa cgucuccgac cuucagcaca ucggaggcuc uaaguacagc     4080

gggguggcca aaauuggcaa uaagaucucc ucucuggaca acugcauccu gauuuaucag     4140

agcaucacgg gaauuuuuga gaaacggauc gaucuccuga agguuuga                  4188


<210>  4435
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4435
auggacagcu ccaagcaggg ccugagacgg uaccaccucg ggcuggauau cggaaccaac       60

ucuauuggcu ggacagccau cgacgaucau uucagccuuc ugcgcgugaa agggaagaau      120

gcuauuggag ucagaacguu uaaagagggc gaaaccgccg cggaccggcg cggguucaga      180

acagcucggc gcagacucuc ccggcgcaga uggcggcugc aauuucuuga ugaguucuuu      240

gcccccuauc uggccgaagu ugacccuaac uuccucgcuc gccugaagca gucugauauc      300

agcgccaaag acccggcuaa gaaucaggag uacaugggaa agcugcucuu ucccgaucag      360

gaagccgcgu ccgacggcaa cggcuauccu acccugauuc aaaugagaaa agccaugccc      420

gcugagaagg ccgcugauuu cccuguguuu aauaucuacc agcuucggua ugcccugaug      480

aacgagaaac gcaaguucga ccucagagaa aucuaccugg cggugcacca uauugucaaa      540

uaucggggga auuuucuuaa caauacaucu guggauaagu ucaaggccga ccagaucgac      600

uuuaacaaga gcuucgagac ccugaaugau cucuucagac agguucaacc cauugcuccu      660

uuucagaucg accugacaaa gguggaugaa cugggaaaac uccugcuuga caccaagcag      720

aaaaagcugg aucgccagaa agccgucgcu aagcuccugc cgcuuauuga ggacggcacg      780

gauaaggaga ucgugaaagc ccaccaacag accgcccggg aaauuuccaa gcugauccuc      840

ggguacaaag cuaagguugg acugauccug cagaacaauc ucgaccaaac acuggauaug      900

agcaccgaga acucugacga ucagcuuaug cagauugcgg aagaacugga cgacuaccag      960

auggaacuca ucaaucaacu gucccuucug uauagccaga uuaugcucaa cgagaucgug     1020

cccaacggcg agacagucuc ugccuccaug cugaauagau acuauaaaca ucgcugccag     1080

cugaaggaac ucaaggauua cggggcugcc caggacaaaa agacccggga gcaacuggau     1140

caccuuuaug ccgaauacau uggacagauc ccuaaagaca gcaaguucga uuuuacgaaa     1200

gaccugaaga agcucaugga uaaaucugac cugggccaga agaucaaaga ugagauugaa     1260

gcuggcgacu aucugcccaa gcagagaaca uccgccaacg gggugauccc ucaccaaaug     1320

caucagcagg agcuugauca gauuaucgag aaucaaaaag aauacuaccc cuggcucgcg     1380

gagccgaacc cuguggaaaa gaauauuaag aacgcuaaau auaagcugga ccagcugguc     1440

agcuuccgcg uucccuacua ugugggaccu augaucaccc ucgccgauca gcagaaaaca     1500

uccaaggccu cuuuugcuug ggccgugcgg aaaaaggcug gcaaaaucac ccccuggaau     1560

uucuacgaca ugauugacag agaaaagagc gccaacaauu uuaucaagcg caugaccaac     1620

aaagauacau aucugcuuuc cgaagacguc cugcccgcga gcucucuccu guaccaaaag     1680

uucacggugc ugaaugagcu uaacaauauu cggguuaacg ggagaaaacu ccagccugcc     1740

cugaagcagg cuaucuauac cgaucuguuu caaaaacgca agacagugaa ucucaagacc     1800

cugacaaacu uccuugccau ucacgagccg acccugacga aacccaagau cgaaggacuc     1860

uccaacggcc ggagcuuuaa uucuagccug gggacauaca uugacauguc caaaauccug     1920

ggagauucuc uugacgaucu caacuacaga gacgauaucg agaagauuau cgaauggagc     1980

accgucuucg aggacuccga gauuuauggc gugaaacugc aggaaaucca guggcugacc     2040

aaugagcagc gggaaaagcu cguucgcaag agauaccaag gcugggggcg gcugagcaaa     2100

aagcugcuua caggaauuac cgaugagaac ggccagagaa ucaucgaccu caugugggau     2160

acaucuaaaa auuuuaugca gauugugcau cagccugucu ucgaggagca aaucgcugag     2220

cugaaccagc agcugcuuca agacgccgac aaggcgcccc ucgaaguggu cgaugacauu     2280

cuggccgaug cuuauacguc cccucagaau aaaaaggcca uccgccaggu gguuaaggug     2340

gucgcugacg ugguuaaagc cguggggaac gcccccgcua gcauuucuau cgaguucgcg     2400

cggaaugaag auagaaacaa ggagcugaaa aauucccgca gacacagcau cgaaaagcuc     2460

uaccacgaga ccgccgagaa acuggucaag cagacaaacc uucuggagga gcucggaaau     2520

gugaaagacc ugaacgaucg guauuaccug uauuuuaccc aagcuggccg cgacauguac     2580

gaugggacac cgauuaauau cgacgaaauu uccacccauu acgagaucga ucacauucuu     2640

ccucagucuu uccugaagga caacagccuc gacaacagag ugcugguuaa gaaauccgaa     2700

aaugccgcca agucugaucg gguccccgcu aaacucuaug gcgccaagau gcgcgcguuu     2760

uggaaacagc ugaaggagag ccagcuuauc uccaacagaa aguaccugaa ucucacgacc     2820

gacccugagc aaguggauaa auaucagaug cagggauucg ugcaucggca gcuggucgaa     2880

acacgccaaa ucauuaagcu ggcugccaac aucuuuggca gccuuuaccc cgacucugcc     2940

gugauugaga ccagagcuga acugacaaaa cagcuccggg agaaguucuc ccuguauaaa     3000

guucgcgaug ugaaugacua ccaccaugcc gucgaugcgu aucucaccgc uuuugccggg     3060

cacuaccugu accagagaua uccuaaacuu cggcccaugu ucguguacgg agacuaugcc     3120

aagaucuacg cugaugaccu gaaaagacuc cgcagcguua accuguuuca cgaucuggag     3180

aaggaccagu auaauucugg cacggccgug aacccggaaa caaaagagaa gauugucgau     3240

guggacuggc uuuccgacua cguuagcaaa auccaacauu ucaaguauau gcugauuucu     3300

aaggcugugu acacccagca cgggcagcuc uacggagcca caauccugcc ugucgcgcaa     3360

guggauaaaa ucagcgcccc cauuggcauc aaggacgaua aacccgugga cauuuauggg     3420

gcuuacacca augaagucgc cgcuuauaug gccaucguuc gggugaacgg caagaaaccu     3480

aaguacaagg uggucuccgu gccgguuaga augcuccagc gccugaaaau ggccgagcag     3540

aagggacaug aucaguauau gcaaacccuu cacgacgugc ugcugcggaa uuuuacaagc     3600

cucaaaaaga acagaaaaac gggccugaag gaagcugucg ugaaagauuu cgacguucuu     3660

gugcccugug uccgcuacaa ucagcuggug auugaugggg acaccaaguu uacacucgga     3720

ucugcgaccu aucagcacaa cgccaagcag cugguucucu gcaaucauuc ccuggagaca     3780

cuugcuaaca acuucgagua cgugcggaaa cacccugaug aagccgacga gagaaugauc     3840

augcuguacg augacauccu ggaccaaguc aaucauuauu ucacccucua cgauaagaac     3900

aaauuucgcg aaaagcugaa ugccggccgg gacaaauauc uuaagcugcc cacguucagc     3960

caguuugaga acaauaagaa aacagcuauu gggaaguccg aagccaucau uaacauccuc     4020

agaggacugc acgcgaaugc ugccauuacc aaccucaaau gucugggcgu gucuaccccu     4080

uucgggcagc uucagagccc cuccggcauc gagcugucug agaaugccaa gcugguguac     4140

caaagcccua caggacucuu uucccgggaa guccugcuua aagaucugug a              4191


<210>  4436
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4436
auggccaacc ugagaaauua caagggcgac uauaacaucg ggcucgauau gggaaccggc       60

agcguggggu gggcugucac agacuccgag ggaaaacugc uucacuucaa gaaacagccc      120

acguggggcu cucggcuguu ugauagcgcc gaaaccgcgg cuuccgcccg cauuccuaga      180

gggcaacggc gcagauacgu ucggcgcaga uggcggcucg accugcuuca gcagcuguuc      240

caggaugagg ugucuaaggc cgacccggaa uuuuucaucc gccucagaca aagccggcug      300

cugaaagagg aucgcgaaga gggacauaga gacuauaauc acccccucuu uaacgauucc      360

gacuucacag agaccgauua cuauaaucgg uuuccuacaa uuuaccaucu gcgcaagugg      420

cuuauggaaa ccgacgagaa ggcugauauc agacuggugu aucucgccgc ucacaacauu      480

gucaaacauc ggggcaauuu ccugagacag gaaaacacau cucuuagcgc caagaaugcg      540

cgcccccugg acgcccuccg ggaguuuaga uccgcucugg uggacuggug cgccgagcug      600

ggcuacgaau guaacccuga ugagagcggg gaaaucucua augagauccu cuccguucug      660

gaagaggagc acguggcuaa cagcgacaaa aagaaacgca uuucuucccu uaucggaguc      720

gccaugaaug aaggcgcgaa ggagagcaag gccugcaacg augcucuggc cggggcuaug      780

gugggacuca aagccgaauu uaagaauguu uuuggcgaga uuucugccga acagaccucc      840

aucuaccuga gcaacgacga ggcuguggau gcgcuucggg aggggauucc cgacgaagga      900

cuggaacucu ucggcaaacu guguuccguc uauucugccu acgugcugca gggccuccug      960

agcuaugcuc cugggcaaac gaucuccgug aacauggucg ccaaguacga aaaauaugga     1020

aaugaucuua agacccugaa agcccucguu ugcgaguacg cuagagccga auaugacagc     1080

uuuuuccgcg gcgaguucua ccgggaucag gacgggcacg agcugaacau uuacaaugcg     1140

ucuaaggcuc agggauauac aagauacaac cuuggcaccu ccaagaugag cuaugaagau     1200

uuugagaaag ccgugaagaa acuguucgcc gggacagcug ccgaagagga cggacgcuac     1260

cuccagauga ugaaggaguu uggcgaacaa cgguuccuga gacgccugaa aaccucugac     1320

aauggcagca ucuauuacca gcuccaucug gaggaacuua cggcuauccu guccaaccag     1380

gggaagcacu auccguuucu cguggaggau gccgaaaaga uuaaaucucu ggucagcuuc     1440

cggauccccu acuacguggg accucugaca gacagaaaug cgcggaagga uaaacauggc     1500

aagaaccgcu uugccugguc cgagagaaaa agcgggaugg aggacgcugu uauuaccccc     1560

uggaauuggg augaagugau cgaccgggga aagucugccg aggcuuucau uacaagaaug     1620

accggcaugu guaccuaucu ucaaggggaa gauguccucc cuaagugcuc ccugcuguac     1680

gaggaauuuu gugugcucaa cgagcugaau ggcagccgcu ggggaaugga cggcgauaaa     1740

gagcaccggu ucgacgccgc ggauagagaa gccaucaucg aggaacuuuu uaagaaaaag     1800

aaaacaguua aguauaagga cguggcuaac uggcuggagc gcgaagccgg gagaaugaau     1860

guccacguga ugggaggcca gggggagacg ggauacgagu cuaaacucuc cagcuauuac     1920

uucuuucggc gcgauauuuu cgacgucgag gagcuggaca gagcugauua ucccaugauc     1980

gaacagauua uccuguggaa cacccuuuuc gaggaccggc gcauucucaa ggagaaacug     2040

gaagaugccu acggcccgaa ggggaauggc agacuggacg augaccagau caaaaagauc     2100

ugccggaaac gcuuuacagg auggggcaga cucuccaaga aguuccugac cgggauuaaa     2160

guggauacag acuguggacc ucggucuauc auggauguuc uucgcgaggg cgaccccaac     2220

gccggguuua gaggaaagac cauggugaug auggaagucc ugcgggauga gaaccucggc     2280

uuccaacaga aaguggacga auuuaauaag aacuacuucg ggcagaaagg caaggcucug     2340

gagguuaaug agcugccugg aagccccgcg auuagacgcu cccuuaacca ggccauccgg     2400

auuguggaug aaaucgcuaa gauugccggc aaagaccccg agucuaucuu uaucgaaguc     2460

acgagagacg augagcucga caagcgcggg aaacggacaa agagacgcua ugaugcccug     2520

aaagaagcuc ugaccguguu caagaaggag gacccugaga uuugggccga acuccaagcg     2580

accagcccgu ccgaucugga cgagcggcuu acacuguacu uuaugcagag aggaaaaugc     2640

cucuauagcg gcgaacccau cgauauuaau aagcugucuu ccggggagua cgaaguggac     2700

cauaucauuc cucgcagcua uaucaaagau gacucucugg agaacaaggc ucuuguuugu     2760

cggaaauaca aucaguccaa gaccgaugcc augcuccugg accccagcau cagacggaag     2820

augcgcucuu auugggacau gcugcucgau gccaaacuga uuggaccuaa gaaacacaga     2880

aaccuucugc gggaccauau cgaugagaag gcuaugaaag gcuucgucgc ccgccagcuc     2940

guggaaacaa gccaauccgu gaagcuggcg gggagccugc uuucugucag auaccccaau     3000

gcuaacauug ugccgguuaa agccuccaug agccacgacc ugcgggaggc cgcuggcuuu     3060

gugaagugca gagaagccaa ugauuuccau cacgcucacg acgccuaccu cgcgugccgc     3120

augggacugu uuauccagau gcggcauccu aaggucuaug agaaccccau ugccaugacg     3180

agagugaugc agaaauacgc ucagcaacag uccaaggagu ucaaucgcaa ccaccggguu     3240

ccuggcaccu cuggguucau cgugaauagc uuuauguccu cuggauucga uaaagaaaca     3300

ggcgagaucu uuaaggacac cuggaacgcc gaaacagaga uugaagggau cagaaaagcu     3360

cucaacuauc gccaguguua cauuagccgg augcccaugg aggauaccgg agccuucugg     3420

aaugccacgg ucuauucccc uagagacccc aagaugggcc cgaagcuggc gcuuccucug     3480

aaaaagggcc ucgaucccaa aaaguacggg ggauuuucua gccaacaguu cgcuuauuuc     3540

uucguguacg aggcccgcga caaaaaggau aagcagguuu uuagauucgc ugaagugccc     3600

gucuggcugg ccucccagau cgagagcgac aaaauugccc uggaacggua cgcuaccggc     3660

cuugccgagg cggaaaagcu gaaauuugug cgcaucgaga gagcuaagau ccucaaaaag     3720

caacugauug agaucgacgg ggaucgguuc auucucacag gaaagaaaga aaugcgcaac     3780

ggcagagagc uggcccuuuc ucagaaggaa acccugaauc ucaucguggc cggggacgag     3840

aacgcuuccc uggaugaccg gggaaaugcc agcgaugcgg ucuucgacgc uauuguuaga     3900

cagggcgaug aaaucggccu gcgccuuuau aaacagcuga agauugccga gaucgccgag     3960

cgguacagau cugugggggc ugacgccaaa gugggagcug ucaugucccu ccugagcucu     4020

aucaacgccu ccagcaauau ggugaaccuc uccaugguug gcgggagcaa guaugcggga     4080

aaucugcaac cuaacuacuc uaaucuucug agcgauccgg aaacaaacuu ugugauuauc     4140

gaccaguccg ucaccggcau guucgagcgc cggacgagaa uugggcugug a              4191


<210>  4437
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4437
augaccaagc ccuacagcau cggccuggac auugggacaa acuccguggg augggccguc       60

aucacggaug acuauaaagu uccugcuaag aaaaugaagg ugcucggcaa uaccaacaga      120

gauuacauua aaaagaaucu gcuuggggcc cuccuguucg acggaggcga gacagcggaa      180

gggcggcgcc ugaagagaac cgcucggcgc agauauacac ggcgcaaaaa cagacuucgg      240

uaccugcagg agaucuuugc cgaagagaug augcaagugg augaaucuuu cuuucagcgc      300

cucgacgaua gcuuccuggu cgaggaggac aagcagggau ccaaauaucc gauuuuuggc      360

acccugaagg aagagaaaga auaccacaag aaguucaaaa caaucuauca ucucagagag      420

gagcuggcca auucuaagga aaaagcugau cuucggcugg uguaccucgc ccuggcucac      480

augaucaagu uucgcggcca uuuccuuuac gagggggacc ugaaagccga aaacaccgau      540

guucaggcgc ucuuuaagga cuucguggag gaauaugaua aaacgauuga ggagagccac      600

cuguccgaaa ucaccgucga cgcccugucu auucucacag agaaggugag caaguccagc      660

agacuggaaa aucuuaucgc ucauuacccc accgagaaaa agaacacacu guuuggaaau      720

cucauugccc ugucucuugg ccugcaagcu aacuucaaaa ccaauuuuca gcucuccgaa      780

gacgccaagc ugcaguucag caaagauacg uaugaggagg accuggaagg gcuccuggga      840

gagguuggcg augaauacgc ggaccuuuuc gugucugcca agaaccugua ugaugcuauc      900

cuccuguccg ggauucuuac agucgacgau aauagcacca aggccccucu gucugcuucc      960

auggugaaac gguacaagga gcacaaagaa gaacucgccg ccuuuaagcg cuucaucaaa     1020

gagaagcugc ccaagaaaua ugaagagauc uuuaaggacg auacaaagaa cggauacgcu     1080

ggcuacgugg gcgcggacaa gaaacugaga aagcggagcg ggaagcucgc caccgaagag     1140

gaguucuaua aauacgucaa gggcauucug aauaaaguug aaggagcuga uuauuuucuu     1200

gacaagaucg acagagagga uuuccugcgc aaacagcgga ccuuugacaa cggguccauu     1260

ccucaccaaa uccaucucca ggaaaugcac gccauucuga gacgccaggg agagcauuac     1320

cccuuccuga aggaaaacca ggauaaaauc gagaagaucc uuacauuucg gauuccuuau     1380

uacgugggcc cgcucgccag aaaggggucu cgcuucgcuu gggccgagua uaaagcggac     1440

gaaaagauca cgcccuggaa uuuugaugac auucuggaua aagaaaagag cgcugaaaaa     1500

uucaucaccc ggaugacacu gaacgaccuc uaccugccug aggaaaaggu gcuucccaag     1560

cacuccccuc uguacgagac cuuuacaguc uauaaugagc ucaccaaagu gaaguacguu     1620

aacgaacaag gagaggccaa auucuucgau acgaauauga agcaggaaau uuuugaccac     1680

guguucaaag agaacagaaa ggucacaaag gauaaacugc ugaauuaucu uaacaaggaa     1740

uuugaggagu uccgcaucgu gaaucucacc ggccuggaca aagaaaacaa ggccuuuaau     1800

gcuagccugg gcaccuacca ugaucuccgg aaaauccugg acaagucuuu ccuugacgau     1860

aagguuaacg agaaaauuau cgaagacauu auccagacac ugacccucuu ugaggauaga     1920

gagaugauuc gccaacggcu gcagaaguau uccgacaucu ucacaacgca gcagcugaaa     1980

gaacuugaga gacggcacua caccgggugg ggacgccuca gcuauaagcu gauuaauggc     2040

aucagaaaca aagaaaauaa gaaaacaauc cuggauuacc ucauugacga uggguacgcc     2100

aaccggaacu uuaugcaacu gaucaaugac gaugcucuuu cuuucaagga ggaaauugcc     2160

agagcgcaga ucauuggaga cguggaugac aucgccaacg ucgugcauga ucugcccggc     2220

agcccggcua ucaagaaagg gauucuccag uccgucaaga ucguggacga gcugguuaaa     2280

gugaugggcc acaauccuac caacauuauc auugagaugg cccgcgaaaa ucagacaacc     2340

gacaagggac ggagaaacuc ucaacagcgc cugaaacuuc uccaggauag ccugaagaau     2400

cuggacaacc ccgucaauau caagaacgug gagaaucagc aacuccagaa cgauagacug     2460

uuucuuuauu acauccagaa uggcaaagac auguauacgg gggaaacccu ggauauuaac     2520

aaucucuccc aguacgacau cgaucauauu aucccucagg cuuucauuaa ggacaacagc     2580

cuggauaacc ggguucugac acgcucugac aaaaauagag gaaaguccga ugacgugccc     2640

agcaucgagg ucgugcacga aaugaaaucu uuuuggucca aacuucugag cgugaagcuc     2700

aucacccaac ggaaauucga caaccugaca aaggccgagc gcggcgggcu caccgagaau     2760

gauaaagcgg gauucauuaa gagacagcug guugaaacgc ggcaaaucac aaaacacguc     2820

gcccaggugc uugacgcucg cuuuaacgcc aagcaugaug agaauaagaa agugauuaga     2880

gacgucaaga ucauuacccu gaaauccaac cucgugucuc aguuccggaa ggauuuuaaa     2940

uucuauaagg uucgcgaaau caaugacuac caccaugcuc acgaugccua ucugaacgcc     3000

gugauuggca cagcucugcu uaagaaauac cccaagcugg cgagcgaguu ugucuacggg     3060

gaguucaaaa aguaugacgu gagaaaauuu aucgccaagu ccgauaaaga gaucggcaag     3120

gcuaccgcca aguacuucuu uuauucuaau cucaugaacu ucuuuaaaaa ggagguuaaa     3180

uucgccgacg gaaccguggu cgaacggccu gauauugaga caagcgaaga cggcgagauc     3240

gcuuggaaua agcagacgga uuucaaaauu gugagaaagg uucuguccua cccgcaagug     3300

aacaucguca agaaaaccga ggugcagaca cacgggcucg accgcggaaa gccccggggc     3360

cuguuuaaug ccaacccuag ccccaaaccu aagcccgacu cuuccgaaaa ucuugugggg     3420

auuaaaagaa accuggaucc gaagaaguau ggaggcuacg cggggaucag caacucuuau     3480

gcuguccucg uuaaagccau cauugagaag ggcgugaaaa agaaagaaac cauggugcug     3540

gaguuccagg gaaucuccau ucuggaccgc aucacauuug aaaaggauaa gcgggccuuc     3600

cuucugggca aaggguacaa ggacauuaaa aagaucaucg agcucccuaa auacagccug     3660

uuugagcuca aggauggauc uagacgcaug cuggcuagca uucuuuccac caauaacaaa     3720

cggggcgaaa uccauaaggg gaaugagcug uucguccccc agaaguuuac gacacugcuc     3780

uaucacgcca aaagaauuaa caauccuauc aacaaggacc auauugaaua cgugaaaaag     3840

caccgcgaug acuucaaaga gcugcuuaau uauguucugg aauuuaacga gaaguacgug     3900

ggagcgacca agaauggcga gcggcucaaa gaagcugucg ccgauuucga cagcaagucu     3960

aacgaggaaa ucugcaccuc cuuucugggg gccgugaaua gcaaaaacgc uggccucuuc     4020

gagcugacau cccuuggauc ugccagcgau uuugaguucc ugggcguuaa gauucccaga     4080

uaucgggacu acaccccuuc cucucugcuc aaagauagca uccugaucca ucaauccauu     4140

acagggcuuu augagacgcg caucgaccug ucuaagcucg gagaggacug a              4191


<210>  4438
<211>  4191
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4438
augaagagag ugaacgagga cuacauccug ggccucgaua uugggaccaa uagcugcgga       60

ugggccguca cagacaaaaa gaacaaucug cuuaaacugc ggggcaagac ggcuaucggg      120

ucccaccucu ucgaagaggg acauaccgcc gcggaucgca gaggcuuucg gacaacccgc      180

agacggcuga aacgcagaaa guggcggcuu cgccugcucg aagagauuuu cgcugaaccc      240

auggccaagg uugacccugg guuuuucgug agacugcacc agucuugggu gagcccgcug      300

gauaaagacc ggaagaaaua uaacgccauc gucuuuccca cagcuaagga ggaucaagcc      360

uucuacaaac auuaugcuac cauuuaccac cuccgcgacg agcugaugac acaggauaga      420

caguuugacc uucgggaaau cuuccuggcc auccaucaca uugugaagua ucgcggaaau      480

uuucuccagg auaccccugu uaaggacuuc gaggcgucca aaaucgaagu gggccccauu      540

cugucucaca ucaacaaugc cuuugcugag aagauugucg aggaucaaga cccuaucgaa      600

cuuaacgugg ccaaugcugc cgacauugag gauguuauca gaggcaaaga cgcggaaaag      660

acgguguaca aacuggauaa ggucaaaaag aucgccaagc uccugaccga cagcacagcu      720

aaagaggaaa agaacguggc caaacagauu gcuaaugcca ucauggggua uaagacccag      780

uucgagacaa uucuggauaa agagaucgac aaguccgaua aggcccagug ggaauuuaaa      840

cucucugacg cugaugcgga cgauaagcug gacgcccuuc ugcccgaccu cgaugagacc      900

gaccaaacgg uggucgcuga aauugagaaa cuguucagcg ccaucacacu uuccaccauc      960

guugaugaaa acaagagccu gucucagucc augguggaga aauacaagaa gcauaaaaag     1020

gacuacaaaa agcucaaaaa guauauuaau acacugcagg aucagaccaa ggccaaaaag     1080

cugcuccugg cuuacgaccu uuaugugaac aaucggcacg gaagacugcu cgaggccaaa     1140

aagaccuucg aagauaaaaa gaaaaagaag gcgcugacaa aagacgaguu uuacaagauc     1200

gucaaagaua accuugacga uagcgaccug gcucaugaaa uucaacagga gaucgccgcc     1260

gauaacuuca ugccuaagca gcgcacgaau agcaacggcg ugauuccguu ucaacuccac     1320

cagaucgaac uggacaaaau cauugcuaau caggggaagu auuaccccuu ccuggccgcu     1380

gagaacccug uugaggacca ccggaagcag gcccccuaua aacucgauga acuggugaga     1440

uuucgcgucc cuuacuacgu gggacccaug aucaccgcgg acgagcaaga aaagacaucc     1500

ggcaaaucuu ucgccuggau gguucggaag gaggaugggc agauuacccc guggaauuuu     1560

gagcagaaag uggacagaca ggaaucugcu aacaaguuca ucaagcgcau gacaauuaaa     1620

gauaccuauc uucuguccga ggacguccuc ccugccaaua gccugcugua ccaacgguuu     1680

gaagugcuua acgagcucaa uaacaucaga auuaauggau cccgcaucuc ugucgaucug     1740

aagcagcaga ucuucaacga ccuguuugaa gagaaaaaga cggugacaga gaaaagccuc     1800

accuccuauc ugaagcagaa ucuucaucug cccaccguug aaauuaaggg ccucgcugau     1860

ccuacaaaau ucaacagcuc ucuggccucc uacuaucacc uuaagagccu gcauguguuu     1920

gacaaagagc ucgcggaucc ccaauaccag aaggacuucg aaaagaucau ugaguauucu     1980

agcaucuucg aagauaagaa aauuuuucag gacaagcugc acgccgaguu caaguggcug     2040

acccccgagc aguuuaaagc uaucuccaca uggcggcucc aaggcugggg gagacugucu     2100

cgcaaacuuc uggucgaacu ccaugacacg aauggacaga acaucaugga gcagcugugg     2160

gauagccaga aaaacuucau gcaaauugug accgaaccug acuuuaagga ugccaucgcu     2220

aaagagaauc agaacguuac acgggccaau ggcguggaag agauucuggc cgacgcuuac     2280

accuccccgg cgaacaagaa ggccaucaga caggucguga aagugguugc ugaugucgug     2340

aaggccgccg ggggcaaaaa gcccgcucaa uucgccauug aguuuacacg ggacccugau     2400

aaaaaucccc agcuuagcca cauccgcgga accaagcucc ugaaggcgua ccaggaaacg     2460

gcuggggagc ugguggacca gaaacucacc gauucucuga aggaagccau gacauccaga     2520

aaacuucuga aggacaaaua uuuccucuac uuuaugcaag ccggacggga ugcuuauacc     2580

ggccagaaga ucaacauuga cgaggucagc acaaauuacc agaucgacca cauucugccu     2640

cagucuuuca ucaaggauga cuccuuugau aacagagugc ugaccgccac gccccuuaau     2700

gcggagaaaa gcgacgaugu uccguauaag cgcuucgcua acaauuacgu guccgacaug     2760

aaaaugacag ucggcgaaau guggaagcau uggcaaaaag ccgggauuau caacaagcac     2820

aagcucggaa aucugcugcu cgauccugac cggcugaaca aauuucagaa gucuggcuuc     2880

aucaauagac agcuugugga gaccagccaa auuaucaaac ugguuuccgu gauucuccag     2940

aacaaguauc ccgaugccga aaucauuaca gucaaagcug gggacaacuc ugcccugcgc     3000

cagagacuga aucuuuacaa gagccgggau gugaacgacu accaucacgc uaucgaugcc     3060

uaucugucca uuaucugugg aaauuuccuc uaccagguuu auccuaaaua ccgccccuau     3120

uuuguguacg gcaaguauaa gaaauucagc caagacccug accugcagaa ggaggucauc     3180

aaacacuuua agggguucac cuuuaugugg ccccuccugc agaaagauaa cucugaaaga     3240

aaggcgccgg agaagauuaa agagaauaac uccgaccgga ucguguucua caagcauccu     3300

gauauuuuug acaaacuucg caaggccuac aauuauaaau acaugcuggu gagcagagaa     3360

accacaacgg agaacucugg ccucuucgau gucaccaucu auccccgggg agaacgcgac     3420

cuggcuaaga caagaaagcu gauucccaaa uccaauggcc uugauccuaa gaucuacggg     3480

ggauauagcg gcaacaccga cgccuacaug guuaucguga aaauugauaa ggggaaagag     3540

ucuaucuaua aggugauugg agucccgaug cgggcucugg ccagccucaa ucgcgccaag     3600

aaacagggca acuacaagga ggaacugcac caagugcucg agccccagau cauguuugac     3660

aaaaauggga agccuaaaag auccguuaag ggcuuccgga uuaucaaaga ucaugugccc     3720

uuuaagcagg ucgugcugga cggagauaag aaauucaugc uuaacagcuc uacauacgaa     3780

aucaaugcua agcagcugac ccucacgccu gagacaauga gaauuguuac cgacaaccug     3840

aaaaagggcg aagaccaaga ucagcugcuu gugaaagcgu augacgagau ccugcagaag     3900

gucgaucagu accucccccu guucgacgug aacaaguuuc gcaauucccu ccaccugggg     3960

cgggccaaau uccuugaucu ggcuguuaac gacaagaaaa uuacccugac aaauauccuc     4020

aacggacugc augauaaucu ugugaccccu gaccugaaga acauuggcau caaaacaccc     4080

cucgggaagc ugcaaguccc gagcggaauu gugcucuccu cugaagccau ccugaucuuu     4140

cagagcccua cgggccuuuu cgagaagaga gugcgcauug ccgaucugug a              4191


<210>  4439
<211>  4194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4439
augaaggacu acaacaucgg ccuggauauu gggaccagcu ccaucggauu cgccgcuauu       60

gacgaugaca auaaccucau cagagugaaa ggcaagaaug ucauuggguc ucggcuguuu      120

gccgagggaa aaacagcggc ugaacgcaga ggcuuccgga cggcccgcag acgguauaac      180

cgcagaaagu ggcggcuuaa acugcucgau gagaucuuuu acgaagagau cauucacauc      240

gaccccgagu ucuuuaaucg ccugaagcag agcuccauuu cuccuaagga ugagagcaaa      300

aaguauuucg ggucccuucu guuuccggac aaaaccgauu cugccuacua ucaugacgga      360

aaccaaacaa ucuaccaccu cagaaaucgg cugguucauc agaaggagaa agcugauauu      420

cgcgaaaucu aucuggcccu cagacacauu gugaaguacc ggggcaacuu ccuggacagc      480

acccccgugu ccagcuuuga ggcugauaau cuuaagcugg acgaacucgu cccugugauc      540

aacgacaugu acgagaauau cgauauugac uucucuauca acguugauaa uauuuccgag      600

aucgaaaaag ugcugcuuga caaccaucuc aagagcgccg auaaacagga ccgccugaag      660

gagcugcugu augauaaucc cgaauacccu gacgauaaag ucaagcagaa agagauuucu      720

aaguuuagaa aggaccucga uaaagaaauc aucaagguga uuguuggcua uaaauucaac      780

gcguacaaga ucgugaacua uauucccgac gacgggucca aauuuagcuu cucuuucucc      840

gaugagagcu ccgacgauaa gcuggacgau cuuucugagg uccuggacga uagccaaaug      900

gaaauccuca auacacugaa ggugauuuac ucccggguga gacuuaacca gaucaucccu      960

aauggaaugg gccugagcga gucuaugguc gccaaauaua accgccacca cgaacagcuc     1020

gaggaccuga aggaacugcu cgauguucug ccgaaugacg agaaacgguc cauugaguac     1080

gcuuacaccg ccuauaucgg gaacguggau aagcagaaau acaauaacaa ucuuguggcu     1140

agccugaagg accuccccau uaagggagau aucaacaaaa agauucaagc cgucuuugaa     1200

uaugcgaaag gccauaucuc uagccaggac cuguacgacg ugauuaagac aaucaucauu     1260

gauaaauauc uugaggacga auccuucgag uaccacgauc ugguugaaag aaucucuaau     1320

gacauugaua acaaggacua ucuccugaag cagcgcagcu cccagaaugg gaacaucccu     1380

caucaagugc accagaugga gauggauaaa auuaucgcca aucagagcca auacuacccc     1440

uuucugggag uccucaaccc uaacgagucu cggagacaca ucgcuaagua uaaaauuucc     1500

gaacugguga gcuuccgcgu ucccuacuau gugggcccgc uuaucacccc ugaggaacag     1560

gagaagacgu cuggcaaaca uuuugccugg augaagcgga aagaggcugg gaagauuacc     1620

cccuggaauu ucgaagagaa ggucgacaaa gaagccacag ccgcuaaguu uaucaaaaga     1680

cugaccguga aggauacaua ccuccugaac gaggacgucc ugccugauaa uucccuucuc     1740

uaucagcagu ucaaagugcu gaacgaacug aauauuguua aggugaacaa uaagcgccuc     1800

acccugagcg agaaacaagg agucuacaac gaccuuuuua agaauacgaa auccaucaca     1860

gugaagcggc ugaaaaacua ucucaucucu aauuacaagc accuggagaa cauugaaauc     1920

accggccuga gcgaugggga caaguucaau aacucccuug gaacauacaa ugacuuuaaa     1980

aagauuuucg gcgcgagaau cgaugacguu aacuauucug ccgauuuuga gaacauuauc     2040

gaauggagca ccguguucga ggaccgcucc auccucaaag aaaagcugga gaaagauauu     2100

gacuggauca cccccgagga aauuaaggcu gucguggcca agcgguacag cgggugggga     2160

agacugucug ccaaacuccu gacagagcuu cuggaugacg auggccagcg gaucauugac     2220

auccucugga augauuccau uacguucaug gaagcuguga gcaagcccga gaucaaagcc     2280

cagauccaga acauuaaucg cgacuuuauc aaggcgaccg acauugaaac aguucuggau     2340

gaggcuuaua ccucuccuca aaacaaaaag gccaucagag aggucuacaa acuggugaag     2400

gacauucaga aggccauggg cgggaaagcu ccguccagca ucucuaucga auuuacacgg     2460

agccccgagg auaauggauc cgugaccaag acgagauaua acaauaugca gaacaaauac     2520

aaggaacuug gcaaagagcu cagcgacgau cugucugccc agcugaagga guauaagggg     2580

cgcauggacc aagauaaauu uuaccucuac uucaugcaga ugggaaagga ccuguaugau     2640

ggcuccacaa uuaaucuuga cgaucugaac agcuaccaga ucgaccauau ugucccucag     2700

uccuuuuaua aagaugacuc uaucgacaau cgggugcuca cccacuacac caacaaucaa     2760

aagaaaggga accauacacc ccugaauggc cugaacauug augcucagac ccagaaccuu     2820

uggaagcacc ucaauaagcu gggacugauc agcaaaaaga aacuccaaaa ccugugcuuc     2880

aaggacauca aucagcuuuc caaauauaca aagcacggcu uuguuagacg ccagcuggug     2940

gaaacgucuc aggucauuaa gcucguggcc aacauccuga gcgaugaguu cgcgaaugac     3000

gggaccaaaa uuaucgaagu uaaggccaaa augaacuccc ugaugagaga uacauuuaau     3060

cuuuacaagg ugcggcaacu gaacgacuau caucacgcug ucgaugccua ccucaccaca     3120

uucgugggaa aauaccugua uaagcgcuac ccuaagcuca gaucuauguu uguuuauggc     3180

gacuucaaaa aguuuagcgc ugaggaugac cugaaagaua uuaagaccuu caauuuccuu     3240

caugacauca cgaaccccaa agaauccaau aaggagaaaa uuuacgauga aaagagcggg     3300

gaacugaucc ucgaccggaa gcacgccauc gagaccauua aaaagaucua ucaguacaaa     3360

uuuaugcuga uugugcgcga agucucuaca aaguccggac agcuguucaa acagaccauc     3420

uauagcgcga agaagacaaa cggccuuaaa ucugccauuc cggugaaguc caaucuggag     3480

ccuagcaucu acggguacag aucuggcaac guggaccacc auauggucau cguuaaaauu     3540

aagaaaggaa ccaaguauuc cuuuaaggug aucggcauua gcgcucgggc cauccaagaa     3600

cucgagauug cuaaaaagau cgggaauuac cgcgauaaac uguaugaggu ggucuccgac     3660

agacucagcc agucuaugaa aagcguggaa uccuuccgga ucauucugga uaacaucaug     3720

uacggacagc uuauuguuga cggcaaugau aaguuuacgc uggggucugc cgacuaucgc     3780

aagaacagca gacagcucgu gcuguguaca aacucccuga agauccuuaa agaugaggac     3840

uacaugaauc ggcugucuga uauucaaaga agcaagcuca ugguccaggu guaugacgaa     3900

aucauuaaaa ucaugaagaa caaauacucc cuguucaucu cuaauaccga gaagcuccug     3960

gaaaaacgcg auauuuucug cggacuuagc uucgacgagc agaagaucgu uauuuggaag     4020

aucauuaucg accugcacaa cggccccuuu acaaaaaucc aagagcuggg gauuaauaac     4080

aagaugggca ccuuccagac caaacaugga aucauucuca cagaaaaugc caucgugauu     4140

uaccaguccc cuagcggccu guuugagcgg aaggucagac uuaaagaucu guga           4194


<210>  4440
<211>  4194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4440
augaagaaag ugcugggccu cgaccugggg accaacagca ucggaugggc ccuugucaau       60

gaggcuacaa aggaagagaa aaaggaaggc aaauccucug agauuaucaa gcuggggguu      120

agagugaacc cccucacggu ggaugaaaag accaauuucg agaaaggacg gccucugagc      180

acaaacgccg accgcacccu uaagagaucc gcgcggcgca aucugcagag auacaaacuc      240

cggcgcagaa accugauuga gauccugauu aagaauggcu uuaucacaaa agauaccccg      300

cucacagaaa ccgggaagga cacgacccac caaacacuga aacuucgggc uaaaucugcc      360

aaggagaaag ucgaucugaa ggacuucgcc aaaauccucc ugaccauuaa caagaaacgc      420

ggauauaaga gcuccagaaa gguuaauucu gaugaagacg gccuugcuau cgauggcaug      480

gccguggcua aacaucugua cgagcagaac cucacacccg ggcaguaugu gcugaaucug      540

cuccagaaga acaaaaagua cgucccugac uuuuaucgga gcgaucugca agaggaauuu      600

aaaaauauuu ggaacaauca gaagaccuac uauuuugaca uccuuacgga uacacuguuc      660

gaggaacuca aggacaaaaa caagucccag accugggcca uuugcaaaaa gcccuuugac      720

aucgcgggaa uuaaacagau cggcaaggcc agcgauaagc gccuggagag auacaaaugg      780

cggacagaag ggcuuaccca aaagcugaac cucgaguacc uggcuaucgc ccugcaggag      840

auuaauaacg acaucaauaa aucuuccgga uaucucggcg cuauuagcga ucgcucuaag      900

gaacuguucu uuaacaaaga gaccgugggg gaaaaucuuu acagacagcu gguuaagaca      960

ccucacacgu cccuccggaa ccagguguuc uauagacaag acuaccugga ugaguuugaa     1020

accaucuggg agacacaggc caaguucuau ccccagcuua cccauacacu gaaagaggaa     1080

auucgcgacg ucaucaucuu uuaccaacgg aagcucaaaa gccagaaguc ucugauuucc     1140

uucugucagu ucgagagcug ggaacagacc uauaucgaga aagaaacggg aaaguccaaa     1200

acaagaaccg ugggccgcaa ggugauuccu aagucuagcc cgcuguuuca agaguucaaa     1260

aucuggcaga aucucaacuc ccuggaguuu cggaauagcg augaaggcga gauuaucgag     1320

uucaucaaac uugacgagga uauuagacag accgucuuug aggaacugaa ccuccgcggg     1380

aaucugaaac ccaacgacgu ucugaagauc cuuggaaauu acauuucucu cggcaaagug     1440

ucccagugga agugcaacuu cgaggaaauc gaggggaauc ggacaaacgc ggcccuguac     1500

aacguguauc aaauuaucgc ugaaaaugag ggauacggcu uugauugggc caagaaaacc     1560

gcuagcgaga uuaacaagga acugaaaaau aucuucccuc agcucgggau cgacgccucu     1620

auucuggacu uugauuucac acaggccaac uuugacaagc agacgagcua ucaacuuugg     1680

caucugcucu acuccgcuga ggaagauuuc aaaaucucug aggaagacaa gguccuguau     1740

ggaaauagcu ccgugagccu gaagaaaaag cuccaccaga aauacggcuu uaagcccgag     1800

uauaccaaac uucugacaaa cauuucucug cagccugauu acggcaaucu cuccgcgaag     1860

gccaucugua acauucugcc cuaccuuaag gagaauaacg uuuauagcga ggcuugcgac     1920

cugaccgggu acaaucacuc uaacuuccuc acagccgaag aacaggccaa aagaccgcug     1980

aagccuaucc uggagcuucu caaaaagaac ucccugcgca aucccguggu cgaaaagauc     2040

cugaaucaaa uggugaacgu ugugaaccag gucauugaga ccuauggaaa gccugaugag     2100

auccggauug aacucacgag agagcugaaa aagagcgcua aggaacgcgc caauaugacc     2160

aaauccaucg cggacgcuac acggagaaac gaugacuacc ggaagaccau uaucaaagag     2220

uucggcaucc ccaaucccac aaagucugau gugguccgcu auagacuuug gcaggagcug     2280

gccagccggg gguacaaaga cauuuuuacc gauacggaaa ucaagccuca agaacucuuc     2340

uccaacaaga uugacaucga acacauuauc ccgaaagccc ugcuguuuga cgauucuuuc     2400

agcaauaaga cacuugcuuu uaaaaaggac aaccucaaaa aggccaauag aaccgcuuau     2460

gauuucaucg ccaacgacua cgcgcccaau cuggagaacu acauugaacg cguggagucc     2520

cugcauaagg aucggaaaau cacaagagcc aagcgcaaua aacuccugau gcuucagagc     2580

aagcugccug acggauuuau ugagagagau cuccggaacu cucaguauau caccaaagaa     2640

gcuaagaaga ugcuguacga gguuugucgc accguggucu ccacaagcgg cucuauuacg     2700

gacaaacuga gagaagauug ggaccuuauc aaugugauga aggagcugaa cuuccccaaa     2760

uauaaggccc ucgggcugac cgaaaagauu gagcgguacg auaucggcgc ugagaagaug     2820

aaggaaguug agaucauuga agacuggaau aaacgcaacg aucacagaca ucacgccauc     2880

gacgcgcuca caguggccuu uuccacccac agccauauuc aguaucugaa ccaccuuaau     2940

gcucggcgcg acgagggaca uaagaaacac gccgcuauca uugccaucga aucuacaauc     3000

accgagaagg agggcaacaa aaagagacgg uucauugccc cucugcccaa uuuucgccuc     3060

caagcuaaag aacauaucga gagcauucug gucuccuuca agaacaagaa uaaaguggug     3120

acgaacaaua ucaacaagac aaaaagaauu aagaaagaua aguuuaauac caagguucag     3180

cugaccccgc gggggcagcu ucacaaagaa acaaucuacg gaaagaucaa acagccucug     3240

gagaagcaca uuaaacucaa caagagcuuc acccuggagc aagcgaagcu caucauugac     3300

aaaagacagc gcgaaaaggu ccugaaucau cuugagaaau augcccacaa cucugauauc     3360

gcuuucaauu ccaagacacu gaaaaagaac ccccuccuga uuagcgacga accucugaag     3420

gaggugcuuu gcuuugaaga gguguucacg auccggaaag auauuuccua cgacaauuuu     3480

aagaacgaga aacagcugga uaaggucauc gacgaaaaag ugaagcaggc ccucaaagag     3540

agaaucaauc aauacaacgg cgauuuccgc gaagccuuuu cugacauuga gcggaacccc     3600

aucuggcuga auaaggcuaa ggggauuugu guuaaaagag ccaccaucaa gggagugagc     3660

aacgcggaac cucuccauac agcuaaaaau caccugggcg aggagguccu uaccgaaaac     3720

aaggagcaga uucccgugga uuauguuucc acagggaaua accaccaugu ggccaucuac     3780

gaagacgagg acggcaaucu gcaggaaaaa gucgugucuu ucuaugaggc cguuauccgc     3840

gugaacgagg gacugccgau uaucgauaag gaauacaaua agagccucgg cuggaccuuu     3900

aaauucacga ugaagcaaaa cgagauguuu gucuucccuu ccgaagacuu uaaucccacc     3960

gagauugauc ugcuugacca gaaaaacgcu ucucugauca gccccaaucu cuuccgggug     4020

cagaaguuca caauuaaaga uuauuucuuc agacaccauc uggagaccac aguggaaaac     4080

aauaacaccc ucaagaacac aaccuggcgc cgggaggggc uguccggaau cuacggcauc     4140

auuaagauca gacuuaauca ccugggggaa auuguccagg uuggagagua uuga           4194


<210>  4441
<211>  4194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4441
augaacgaga auaaggaaug guuccugggc cucgacaucg ggaccgccag cgugggaugg       60

gcugccacag auacgaacua caaucugcuu aaacugaagg gcaaaaaguc cuggggaguc      120

agacucuuug acgaggcgca gaccgcuaaa gaacggcgcg auugcagauc uaagcggcgc      180

agacuggccc ggcgcaagug gagacuuggg cugcucgagg aacuguucgc cgaggagauu      240

aacaaaaucg accccaacuu uuucaugcgg cugcgcgaaa gcaaguauaa aguuuccgau      300

aagacaggca augugccuuc uauucucuuu aaugagaaaa acuacaccga caaggaauau      360

uacagaaauu auaagacaau cuaccaucug cgggcugauc uuauucacaa aaacaauccg      420

gacauccgcg agcuguuccu cgccauccac cauauucuga aguauagagg gcacuuucuu      480

uucgcuggac aacaguuuaa aggcagcgag aucaaggaua acauucggga acugcuccag      540

uacaauuccc ugaacaucaa aauugacauc accgaugagc ugauuaauaa caucgccgaa      600

auccuccuga agaaaaagaa cucuacagac aagauuaucg aguucaaaaa gcuguuugcg      660

gaugaaaaac agcuuauuga ggcccuccgc cuggcucuug gcaauaagac ccaacuggcc      720

aaacucuucg acaucgaggc uuacaaggaa cuggacauua gcaucaagac gaucuccuuu      780

aaagagaaga uuuaugaaga ugugagaagc aacuacgagg aaauccuggg ggaguauauu      840

aucguccucg acaccgccaa acugauuuac gaugcgauca uccuuucuaa gauuaaaauc      900

aagaauauga caauuuccga gagcaaggug guuucuuaug aaaaacaugg aaaggaccug      960

aaacagcuca agcaccugau caaagaggau uguaaacuuu ccaagaagga aaagcggcag     1020

cuguucgaga acauuuucgu gaaagaagac gagaagggcg ccaauuacgu caacuauauc     1080

gggaaaaaua acgcuggaag cucuaagucc agcugcuccu acgaggauuu uaaaaaguuc     1140

cucaccaagg aacugaaaug uugcgaggaa ucuaagcuga aacaggacau ucucgccgag     1200

cuggagcuug aaacauuucu gcccagacaa cgcaccaagg auaauagcgu gauccucuac     1260

cagauccaca aagaggaacu ggagaagauu cugaacaaug cuguucagua ucauccuuuc     1320

cuuaacaaua cggacgaaua cggcaucucc auuaaggaga aaaucauuca gcuccuggag     1380

uuucggaucc ccuauuacgu ggggccucug aacacaagcc acaaggucgg agauggcggc     1440

uucucuuggu ccgugagaaa uaaaggguau gaacauaucg ccgugacccc cuggaacuuu     1500

aacaagguca uuaauaaaga ggaaagcgcc aagaacuuca ucgagaaucu cacaaaccaa     1560

uguaccuacc ugcuuuauga agacguucug ccuaagcacu cucuccugua cagcgaguuu     1620

gcucugcuua augagcucaa cuccauuaau uacggaggca aacgccugac cguggaacac     1680

cggaaccuga ucauugagag auucuuuaag cagucugcgc cgaaagccac agggaaggaa     1740

cucaaaaagu uccugaugag cgagggagug aucgacggca aggaugaacu uuccggguuu     1800

gacgucagca ucaaaaauga ucugaagucu uaucgcgacu ucaaaaagau ucucggagau     1860

aaauucgacu ccgagaaggu ggagaaaaug auccggugga uuacgcuguu uggcgaaagc     1920

aagcagcugc uuguugauaa gaucaccaaa gaguacggcg acauuuauuc ucucgaagag     1980

aucaagaaaa ucuccgggcu gacauacaag gauuggggaa gacugagcga gaaacuccug     2040

accgaaauug uguccucuca acuuuaugac gagagcacag gcgaaugcuu caacaucauu     2100

aaugcuaugc gcaacaauac ccugcucuuu auggagcugc uggggacgaa cuacgauuau     2160

uucgaaaaga ucaauauuua caaccggaga cuucgcaagg agaucacaga gauuacaccc     2220

cauauccucg acgaucugua cgucuccccu gccgugaaac ggucuaucug gcagguugug     2280

agaauugucg aagaacugaa gaaaaucauu ggaagcgaac ccaagcggau cuuuguggag     2340

accacccgcu ccaacaaaga aaagaagggc gacgacggga gaaaagauag ccggaagaaa     2400

gcccuccugg agcuuuauaa gaaaauuaag accucugaca ucgucgagcu ggaaaaggcu     2460

cucggccaca caguggauuu cgacaaaaag aaacaggauc ugacggacau cgauaauaac     2520

aagcugaaag ccagaaaacu uuaccuguau uacacccagc ucggacgcug ucuguauaca     2580

ggcaaggcga uugaccucaa uaaccugcuu uccagcaauu cugaguacga uguugaccau     2640

aucuauccuc aauccaagac caaggauggg agcuggaaca aucuggugcu cgucaacaaa     2700

acagaaaauu cuaagaaaag caacggacug auuuccgaga gcauccagcg gaagaauaaa     2760

cacuuuugga gauuccugaa ggcuaagaac cuuauuucug aagagaaaua caagcgccug     2820

accagaacgg aggacuuuac cgaugacgaa cucuccggcu ucaucgcccg gcagcugauc     2880

gagacaagcc aguccgugaa agccguuucu caaauucucc agaaucugaa ccccaccaca     2940

gaaaucugcu uuagcaaggc ugagaaugug caugacuucc gccaggaguu ugggaaaauu     3000

aaggucggaa agagacggaa ccuuaccgag gagcagcaaa aagaagccaa ggagggccag     3060

cugaucaaag ugcgcgaucu caacgaccug caccacgcua aggaugccua ccugaauauu     3120

gugguuggga acgucuauca uaucaaauuc acgagaaauc cgaagaacuu cauucggaaa     3180

aauuccaaga accgcgaaua cacacuuaau acccuguuuu auucugacaa gaucgagaga     3240

aacggcgagg uggcguggga aaaaggaaag acagagaaaa ucgugagcuc cgucaugugu     3300

aauaaccuca uuaaugugac ccggcgcacc ucugaacaga agggcgcccu guacgaugcu     3360

acaaucuaua aggccaacgc ugccaaagag gggagcuacu auccuguuaa gacgaaaaau     3420

aagaaccuca aagacgugac cagauacgga ggcuucacau ccauuaagau cgcguacuau     3480

agcauuuuua aauacgauau ccggaauaag gacaagguca aagaaaacau caagauuauc     3540

cccauuccua ucuauauuuc ucagaauauc aaaaccgauc uggaccuuau caacuucgcc     3600

gagaagcaaa uuaaacugaa gaagggggag aacaucgaaa aucugcagcu cauuuacaaa     3660

aagcugaaca ucggagcucu ugugaaaauu gauuauuacc ccuauuacgu uggcgggaag     3720

acaaaugaca acuuuuacau cgacggcgcc gugcaggucc ugcucuccag cgauauugag     3780

agauaucuga aagaaguggu uaaguucgug aauuuucaac gcgagaacaa ggaagcuaaa     3840

gucucuaagg agcacaucuc cagcgaggaa aauaucgagc ucuacaacac ccugcuuugc     3900

aaaaugacgg ccaagauuuu cacacauacc aaaaauaaca aguauaauga ccuggcccag     3960

gauaaggcua aaaacaccuu uaagaaucug ucucucgagg aacaguguaa agugcugcuu     4020

gagauccuga accuccugac aaauaagaaa accacauucg accucucccu gauuaacgaa     4080

aaggcgagcc gggugaaaug cggauuuaac cuucugaauc agaagcaauu cuccaucauu     4140

gagcagagcg ucacgggccu guacgaaaag gagaucaaaa ucauugaaga uuga           4194


<210>  4442
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4442
auggagaccc aggccagcaa ccaacugauc uccucucacc ucaagggcua ccccauuaaa       60

gacuauuucg uggggcugga uaucggaaca agcuccgucg gcugggcugu uacgaauaag      120

gccuacgaac uucugaaauu uagaucucau aagauguggg ggagccggcu uuucgacgag      180

ggagaauccg cgguggcucg cagaggcuuu cggucuaugc gcagacggcu ggagcgcaga      240

aaacuucggc ugaagcuccu ggaagaacug uucgccgaug ccauggcuca gguggacccu      300

accuuuuuca ugcgccucag agagagcaag uaucacuacg aagauaaaac aaccgggcau      360

uccagcaagc acauucuguu uaucgacaaa aacuauaaug aucaggacua cuucaaggag      420

uauccgacag ucuaccaucu ucggucugaa cugaugaaau ccggaaccga ugacauucgc      480

aagcucuuuc uggccgugca ccacauccuu aaguacagag gcaacuuccu guaugagggc      540

gcuacauuug auagcaaugc cucuacccuc gacgauguua ucaaacaggc gcuggagaac      600

auuacguuca auugcuuuga cuguaacucc gccaucagcu cuauugggca aauccugaug      660

gaagcuggaa agaccaaauc cgacaaggcc aaagcuauug agcaucucgu ggauacauac      720

aucgccaccg acacagucga uaccagcucc aagacgcaga aagaccaggu gaaggaagau      780

aagaaacggc ugaaggcguu cgccaaucuu guucugggcc ucaacgcuuc ucugauugac      840

cuuuucggga gcguggagga acuggaggag gaucucaaaa agcugcagau cacaggagac      900

accuaugaug acaaacgcga ugaacuggcc aaggcuuggu ccgacgagau cuacauuauc      960

gacgauugca agagcgucua ugacgccauu auccuccugu cuauuaaaga acccggccuu     1020

acaaucuccg agagcaaggu gaaagccuuu aauaagcaca aagaugaccu ggcuauccuc     1080

aagucucugc uuaagagcga uagauccauu uacaacacca uguucaaagu ggacgaaaag     1140

gggcugcaua auuaugucca cuacaucaaa caaggacgga ccgaggagac aucuuguaac     1200

agagaagauu uuuacaagua uacgaaaaag auuguugagg gccucagcga cuccaaggau     1260

aaagaauaca uccugagcca gauugagcug cagauccucc ugccucuuca gcgcaucaag     1320

gacaauggcg ugauucccua ucaacugcac cucgaggagc ugaaagcgau ccuggccaag     1380

ugcgggccua aauuccccuu ucuuaacgag guggcugaug gauucucugu cgccgaaaag     1440

cucauuaaaa ugcuggaguu ucggaucccu uacuaugugg gcccgcugaa cacccaucac     1500

aauguugaca acgggggauu cgccugggcu gugagaaagg ccuccggccg cgucacaccc     1560

uggaauuuug augacaagau ugaccgggag aaaagcgcgg cugccuucau caagaaccuc     1620

accaauaaau guacauaccu gcuuggggaa gaugugcugc cuaagucuuc ccuccuguau     1680

agcgaguuua ugcugcuuaa cgaacucaau aacguuagaa uugacggaaa accccuggag     1740

aaggugguca aggaacaucu gaucgaggcc guguucaaac aggaucacaa gaaaaugacc     1800

aagaaucgca ucgaacaguu ucucaaagac aacggcuaca uuuccgagac gcauaagcac     1860

gagaucacag gccuggaugg ggaaauuaag aaugaccuug cuucuuaccg ggauaugguc     1920

agaauccugg gagacggcuu cgaucgcagc auggccgagg aaauuaucac cgacaucacc     1980

auuuucgggg aguccaaaaa gaugcuccgg gaaacacugc gcaaaaaguu ugcuucuugc     2040

cuggaugacg aggccaucaa aaagcucacc aagcucagau aucgggauug gggaagacug     2100

agccaaaaac ugcucaacgg cauugagggg ugugacaagg cgggcgacgg aacaccugaa     2160

acgaucauua uccugaugcg gaauuucucc uacaaccuua uggagcugcu cggcgauaaa     2220

uuuagcuuca uggaacgcau ccaggagauu aaugccaagc ugaccgaagg gcagaucgug     2280

aacccccacg acauuaucga ugaccuggcu cuuucuccgg ccguuaaaag agcugugugg     2340

caggcccucc ggauugucga ugagguggcg cauaucaaga aagcccugcc ugcuagaauc     2400

uuuguugagg ugacacgcuc caacaagaau gaaaagaaaa agaaagacag ccggcaaaag     2460

agacugucug aucucuaugc cgcuauuaaa aaggacgaug uccugcuuaa cggacugaau     2520

aacgagaucu ucggcgaacu caaguccagc cuggccaaau acgacgaugc cgcucugcgc     2580

ucuaagaaac uuuaucugua cuauacccag auggggagau gcgcguacac aggagagauu     2640

aucgagcuca gccugcucaa uaccgacaac uacgacauug aucacaucua uccccggucc     2700

cugacgaagg acgauagcuu ugacaaucuu gugcugugua aacgcaccgc caacgcucag     2760

aagucugaug ccuacccuau uuccgaagag auccagaaga cacaaaaacc cuucuggacc     2820

uuucucaagc agcagggccu gaucagcgaa agaaaauaug agcggcugac acgcauuacg     2880

ccccuuacgg ccgacgaucu guccggguuc aucgcuagac agcucgugga aacaaaucaa     2940

ucuguuaagg ccgcgaccac acugcuccgg cgccuguacc cuggcgucga cgugguguuu     3000

gucaaagcug agaacgugac cgauuucaga caugacaaua acuuuauuaa gguucggagc     3060

cuuaaucacc accaucacgc caaggaugcc uaucugaaca ucguggucgg aaauguguac     3120

caugagcgcu ucaccagaaa cuuccgggcc uuuuucaaaa agaacggcgc uaauagaaca     3180

uauaaccucg cgaaaauguu uaauuacgac guuaacugca cgaaugcuaa ggaugggaaa     3240

gccugggacg ugaagaccuc cauggacaca gucaagaaaa ugauggauuc uaacgacgug     3300

cgcguuacca agcggcugcu ggaacagaca ggagcccuug cugaugccac cauuuacaaa     3360

gcuacggugg ccggcaaggc gaaagacggg gccuauaucg gaaugaagac aaaaagcucc     3420

gucuucgcug augugucuaa guacggcggg augaccaaga ucaaaaaugc cuauagcauu     3480

aucgugcagu acaccggcaa gaaaggagag gucauuaagg aaaucguucc gcugcccauu     3540

uaucucacaa acagaaauac cacagaccag gaucugauca acuacguggc uuccaucauu     3600

ccucaagcca aagacaucag cauuaucuau ggcaagcucu guauuaauca gcuggugaag     3660

gucaacgggu uuuacuacua ucuuggaggc aaaaccaauu cuaaguucug caucgauaac     3720

gccauucagg ugaucguuuc caaugagugg auccccuacc ugaaagugcu ggaaaaguuu     3780

aacaacaugc gcaaagacaa uaaggaucuc aaggcuaacg ucgugagcac ccgggcgcug     3840

gacaauaaac acacaauuga gguuagaauc guggaggaaa agaacauuga guucuuugau     3900

uaucuugucu cuaaacugaa gaugccuauc uaccagaaaa ugaaggggaa uaaggccgcu     3960

gaacucuccg agaaaggaua uggccuguuc aagaaaauga gccucgagga acaaucuauu     4020

caucugaucg agcuucugaa ccugcucacc aaucagaaga caaccuuuga agugaaaccc     4080

cuggggauca cggccucccg cagcaccguu ggcuccaaga uuagcaacca ggacgaguuc     4140

aaagugauca augaaucuau uacaggacuu uacagcaacg aggucaccau cguguga        4197


<210>  4443
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4443
auggaaaaga gcuacuccau cggccuggac auugggacca acucuguggg auggagcguc       60

aucacagaug acuauaaagu ucccgccaag aaaaugagag ugcucggcaa uacggauaag      120

aaauacauua agaagaaccu gcuuggggcu cugcuuuucg acuccggaga aaccgccgag      180

gcgacacggc ugaaacgcac cgcuagacgg cgcuauacaa gacggaagaa ucgccuuaga      240

uaccugcagg aaaucuuugc caaagagaug gccaaggugg augaaucuuu cuuucaacgg      300

cucgacgaga gcuuccugac cgaugacgau aaaacauuug acucccaccc uauuuucggc      360

aacaaggcug aggaagaugc cuaucaucag aaguuuccga ccaucuacca ucugcgcaaa      420

caccucgcug acucucagga gaaggccgau cugagacuua ucuaucuggc gcucgcccac      480

augauuaaau uccgggggca uuuucuguac gacaauuuca acgacgauaa uuuugacugg      540

cgcaacaucg auauucagaa gagauacgaa gaguucaucg agacguauga cagcacccuu      600

ggagaauccu accuggcuga uauuagcguc gacgccgcuu cuauccucga ggaaaaagug      660

uccaagacag agcggcugga aaaucugcuc aaauauuacc ccaccgagaa gaagacaacc      720

uuuuucggca accugauuaa acuuauccug ggccaacagg ccaaguucaa agcgaucuuu      780

aaucucgagg augaaauuag ccugcaguuc gccacgccua cauaugacga ggaucuugag      840

gagcugcucg ggaagaucga caacggagau ucuuacuccg aacuguuugu ugcugcccag      900

aaucuguaua acaccauucu ccuggcuagc uuccuuaaaa cagacaauaa gucugccaag      960

gcuccccugu ccaccagcau gaucgagcgc uacgagaacc acaaaaagga ccucgccaaa     1020

cugaaggauu uugugaaaaa gaacugcccu gaccaauacc acgauauuuu cagagacaag     1080

uccaaaaaug gcuaugcggg guacaucgau aacggagucu cucagaauga cuuuuauacc     1140

uucaucggca agugucuuga agagagccug aaaaaggaua aaggggccca guacuuucuc     1200

gacaagauug aucgggacga uuuccugaga aagcagcgca cauuugacaa cggagcuuuc     1260

cccuaucaaa uccaucugca ggaaaugcac gccauucucc ggagacaggg cgauuacuau     1320

ccuuuucuga aagagaauca agacaagauc gaaaagauuc uuacguuccg caucccguac     1380

uacgugggcc cccuggcccg gaaggacucc agauucgcuu gggccaacua ucgcagcgau     1440

gaggcgauca ccccuuggaa uuuugacgag guuguggaua aagaaaaguc ugcugagaaa     1500

uucauuacac ggaugacccu caacgaccug uaccugcccg aagagaaggu ccuuccuaag     1560

cauucccuca ucuaugagac auuuaccgug uacaaugaac ugacgaacau uaaauaugug     1620

aaugaucagg ggaacgccau ccacuucgac agcgagcuga aggagaaaau uuuuaaucaa     1680

cucuucaagg aaaacagaaa agucucuaag aagacacuga ucgauuuucu uaauaacagc     1740

gaaggaauuu acaccgacaa acugguuggc aucgaugagg aggugaagua ucucaacgcc     1800

ucccugggga ccuaccacga ccugaaaaag auccuugaau cuuucaugga ugacgagauu     1860

aaugagaaaa ucauugaaga uaucauucag acacucaccc uguuugaaga caucgagaug     1920

aagcgccagc ggcugcagaa guacgacgau aucuucacac ccaaacagcu caaggagcug     1980

gcuagacgca acuauacggg auggggccgg cuuagcuaca aacugauuaa ugggaucaga     2040

aacaaggaaa auaacaaaac cauucucgac uaucugaaga auggaaaccg gaauuuuaug     2100

cagcugauca acgaugaccg ccuuuccuuc aagcaaauua ucaucgaugc cagaaaaauu     2160

gagaagcucg acaauaucga aagcgugguc uacaaccugc cgggcucucc ugcuauuaaa     2220

aagggcaucc ugcaguccau uaaaaucgug gaugagcucg uuaaggugau ggggcauaau     2280

cccgacaaca ucgucauuga aauggcccgg gagaaucaga caaccaacca gggcaaaaac     2340

agaagccaac agcgccugaa gcggcuucag gauucuaugu ccaauuuuaa ggacagcucc     2400

aucucucuga aagaugugga caacagcgac cuccagaaug auagacuguu ccuguauuac     2460

auucaaaacg gaaaggacau guauacaggg gaggaacuug auaucgacca ccucuccgau     2520

uacgacauug aucauaucau uccucagucu uucaucaaag acaauagcau cgauaaccgc     2580

guucugaccu ccagcgcgaa gaauagagga aaaucugacg augugcccgg ccgggacguc     2640

gugcugaaga ugaagcccuu uuggaaaaag cucuacgaug ucaaacugau uuccaagcgc     2700

aaauucgaca accuuaccaa gagcgagcac gggggccuga cggaaucuga caaggccgga     2760

uuuaucaaaa gacagcucgu ggagacacgg caaauuacca aguauguugc ucagauccug     2820

gauggccgcu ucaauacaaa aagagacgau aacaauaagg ugauucggga cgucaaagug     2880

aucacccuga aguccagccu uguuucucag uuucgcaagg auuucggguu uuacaaagug     2940

agagagauca acgacuauca ucacgcccac gaugcuuacc ugaaugccgu cgugggaacg     3000

gcgauucuca agaaauaucc uaagcuggcc ccggaauuug uguacggcga guauaaaaag     3060

ugcgacguuc ggaagcucau cgcuaaaagc ggggauaagu ccgaaauugg aaaagccaca     3120

gcuaaguacu uuuucuacag caaccugaug aauuucuuca aacgcgucau cagauauucu     3180

aacggcauga uuguggugcg gcccgucauc gaguacucca aggacaccgg ggaaaucgcc     3240

ugggauaaag agaaggacuu caagacagug uguaaaguuc uuagcugccc ucaggugaac     3300

auugucaaga aaguggagaa gcaaucccau ggccuggaua gaggaaaacc caagggcuuu     3360

uauaaugcca acccuucucc caagccgaaa aaggggagca aaguuaaucu cgugccuauc     3420

aaggcuaacc ugaaucccaa aaacuacgga ggcuaugcgg ggauuuccaa uucuuacgcc     3480

guccuggugg acgcuaccau cgaaaaggga gccaagaaaa agcucacccg cauucaggag     3540

uuccagggca ucagcauuau cgaccgggaa aaauaugaga agaacaaagu ugaauuucug     3600

aaggggcucg gcuacaagga gaucuacucc auuaucacac ugccuaaaua uucucucuuc     3660

gagcuggccg auggaagcag acgcaugcuu gcuuccauuc ugagcacgaa uaacaagcgg     3720

ggcgaaaucc acaaagggaa ugagcucgug cugcccgcca aguacauucc ucugcuuuau     3780

caugcgaaca gaauccacaa uaccuuugaa acaggacacc gcgaguacgu cgaaaaacau     3840

aucgcugagu ucaaggagau ugccgaaauc auucuggagu uuaacaauaa auaugugaac     3900

gccaagaaaa auucuuccau cauugaaaaa gcucucgaga gcuucgacuc uuuuucccug     3960

gaugagaucu gugacagcuu cgugggcaag cucaaaaaga acaacaccaa aaagaacucu     4020

gggcuguuug aacuugucuc ccugggaagc gccuccgauu ucgaguuucu ggaaacaaag     4080

guuccccggu acagagacua uaccccgagc ucucuccuga augcgacgcu uauccaccag     4140

agcauuacag gccuguacga gacccgcauc gaucucucca aacuggggga agaguga        4197


<210>  4444
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4444
auggagaccc aggccagcaa ccaacugauc uccucucacc ucaagggcua ccccauuaaa       60

gacuauuucg uggggcugga uaucggaaca agcuccgucg gcugggcugu uacgaauaag      120

gccuacgaac uucugaaauu uagaucucau aagauguggg ggagcagacu cuucgacgag      180

ggagaauccg cgguggcucg cagaggcuuu cggucuaugc gcagacggcu ggagcgcaga      240

aaacuucggc ugaagcuccu ggaggaacug uucgccgaug ccauggcuca gguggacccu      300

accuuuuuca ugcgccucag agagagcaag uaucacuacg aagauaaaac aaccgggcau      360

uccagcaagc acauucuguu uaucgacaaa aacuauacag aucaggacua cuauaaggcc      420

uacccgacca uuuaccaucu ucgggcugag cugaugaaag auggaacaua ugacauccgc      480

aaacucuucc uggccgucca ccacauccuu aaguacagag gcaauuuucu guaugaaggc      540

gcgucuuucg auuccaacgc cagcacccuc gacgaugugc ugaaacaggc ucuggagaau      600

auuacguuua acugcuucga cucuuccgcc gcuaucggga gcauuggaca aauccucaug      660

gagaccggca agacaaaauc ugacaaggcc aaaaccauug aacggcuggu ugauacauac      720

aucauuaccg acgaggugaa ugcguccggg aagacgcaga aagaucaggu caaggaagac      780

aagaaacgcc uuaaggccuu ugcuaaccug gugcucggac ugaaugccag ccuuaucgau      840

cuguucggcu ccguugagga acucgaggag gaccugaaaa agcugcagau cacaggggau      900

accuaugacg auaaaagaga cgaacucgcu aaggccuggu cugaugagau uuacaucauu      960

gacgacugua agagcgugua ugaugccauc auucugcuuu ccaucaaaga acccggacug     1020

acaaucagcg agucuaaggu caaggcuuuc aacaagcaua aagacgaucu cgcgauucug     1080

aagucccuuc ucaagagcga ccggucuauc uacaaugcca uguuuaaagu ggaugaaaag     1140

ggccugcaca acuacgugca uuauauuaaa caaggcagaa ccgaggagac cagcugcaau     1200

cgcgaagacu ucuacaagua uaccaaaaag gucguugagg ggcugccuga uuccaaggac     1260

aaagaauaca uccugucuga gauugaacuc cagacgcugc uuccccugca gcggaucaag     1320

gauaacggag ugaucccuua ucagcuccac cuggaggagc ugaaagugau ucuugcuaag     1380

uguggcccca aauuuccuuu ccucaaugaa gucgccgacg gguuuagcgu ggccgagaag     1440

cugaucaaaa ugcuggaguu ccggauuccg uacuauguug gaccccucaa caccucccac     1500

aacguggaua auggcggguu ugcuugggcc guccgcaagg cgagcggacg ggugacaccu     1560

uggaacuucg acgauaagau cgacagagaa aaaucugcug ccgccuuuau uaagaaucug     1620

accaacaaau gcacauaccu ucugggcgag gacguucucc ccaaguccag ccugcuguac     1680

ucugaguuca ugcuucucaa ugaacugaac aaugugcgca ucgauggcaa accucuggaa     1740

accaagguca aggcucaucu cauuaaagcc guguuuaagc aagaccacaa aaagaugacg     1800

aaaaaccgga ucgagcaguu ccugaaggau aauaacuaua uccccgaaaa gcauaaaccg     1860

gagauuacag ggcuugacgg agagaucaag aaugaucugg cuuccuacag agacaugguc     1920

cgcauucucg gcaacaauuu ugaucugagc auggccgaag agaucauuac cgacaucacc     1980

aucuucgggg aauccaaaaa gaugcugcgg gagacacuuc gcaaaaaguu cgcgucuugu     2040

cucgaugacg aagccauuaa gaaacugagc aagcugagau aucgggauug gggaagacuc     2100

uccaaaaagc ugcuuaacgg caucgacggg ugcgauaaag cuggcgacgg aaccucugug     2160

acaauuaucg aggccaugcg ggacuuuagc uacaaucuga uggagcuccu gggcgaucgc     2220

uuccuguuua uggaacggau ucaggagauc aacgacaaac uuacggaagg gcaaaucguu     2280

aacccucacg auauuaucga cgaucucgcu cugucccccg ccgugaaaag agcggucugg     2340

caggcccuga gaauugugga cgagaucguu cauauuaaga aggcucuccc ugcccgcauc     2400

uucguggaag ucacccggag caauaaagcu gagaagaaaa agaaagauuc uagacagaag     2460

cgccuguccg accuuuaugc cgccaucaag aaagaugacg cucugcucag cggacugaca     2520

aacgaggaau uuggcgggcu gaagucugag cuugcgaaau acgaugacgc cgcucugaga     2580

uccaagaaac ucuaucugua cuacacccag augggacggu gugccuauac aggcgaagug     2640

auugaccuca gccaacugaa uaccgauaac uacgacaucg aucacauuua uccccgcucu     2700

cuuacgaagg acgauagcuu cgacaaucug gugcucugca agagaaccgc caacgcucag     2760

aaauccgaua cauaccccau cagcaaggag auucagaaag cccagaaggc guuuuggauc     2820

uuccugaaac aacaggggcu gauuucugag cggaaguaug aacgccuuac cagaaucaca     2880

ccucugaccg cugacgaucu cuccggcuuu aucgcccggc agcugguuga gacgaaucag     2940

agcgucaagg ccgcuacaac ccuccugcgc agacuuuacc cggacauuga ugugguguuc     3000

gucaaagccg aaaacgugac agacuuucgg cacgauaaua acuucaucaa gguucgcucc     3060

cugaaucauc accaucacgc gaaagacgcu uaucucaaca uuguggucgg aaauguguac     3120

cacgagaagu uuaccagaaa cuuccgggcc uucuuuaaaa agaauggcgc caacagaacc     3180

uacaaccugg cuaagauguu caauuaugac guuaaaugua caaacgccaa ggaugggaag     3240

gcuugggacg ugaagacguc uauggauacc gucaauaaaa ugaugacaag caacgacgug     3300

cgcguuacca agcggcugcu ugaacaaucc ggagcccugg cggaugccac aguguacaaa     3360

gcuaccgucg ccgcuaaggc caaggacggc gccuauaucg ggaugaaaac gaagucuagc     3420

guguuugcug augugacaaa auacggaggc augaccaaga uuaaaaaugc guauuccauc     3480

aucguccagu acauugggaa gaagggcgag guugugaaag agaucgugcc ccucccuauu     3540

uaucugacca acagaaauau cacagaccag gaucucauug acuacgucgc cucuaucaca     3600

ccccaggcua aggauaucag cauuaucuac cgcaaacugu gcauuaacca acuugugaag     3660

guuaauggau ucuauuacua ucugggcggg aaaacaaacu ccaauaucua cauugacaac     3720

gccguggaag ucgugguucc ugccaauauc gcuguguaua uuaagguccu ggagaaguuu     3780

gcccuccugc auaaagauaa caauaacauc aaggcgagca cggugaccac acggaucuac     3840

aacgaaggau cuaccaauau uguuuccauc acagacaaag acggccuuau uguguaugau     3900

uaccugauga gcaagcucag aacgccccug uacaugaaaa ugaaggggaa caaggucgac     3960

gagcucucua ccgugggacg cuccaaauuc augaauauga cccuggagga acagagcauc     4020

uaucuucugg aggugcugaa ccuccugaca aauucuaaga ccacauuuga ugucaaagcu     4080

cuuggcauua ccgccucccg gagcacaauc gggguuaaga uccacacccu ggacgaguuc     4140

aaaauuauca accaguccau uacgggccuc uacagcaaug aaaucacaau uguguga        4197


<210>  4445
<211>  4197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4445
auggagaccc agacaagcaa ccaacugauc acgucccacc ucaaggacua ccccaaacag       60

gauuauuucg ugggccugga cauugggacc aauucugucg gaugggccgu uacaaacacc      120

agcuacgaac uucugaaguu ucauucccac aaaauguggg gcucuagacu cuucgaggaa      180

ggggagagcg cugugacacg gcgcggauuu agauccaugc ggcgcagacu ggaacggcgc      240

aaacuuagac ugaaacuccu ggaggagcug uucgccgaug cgauggcuca gguggacucu      300

accuuuuuca uccggcucca ugaaagcaag uaucacuacg aggauaagac aaccggccau      360

uccagcaaac acauucuguu uaucgacgaa gauuauacgg accaggauua cuucaccgag      420

uauccuacaa uuuaccaccu ucgcaaggac cugauggaga augggaccga ugacaucaga      480

aaacucuuuc uggccgucca ucacauccuu aaguaccggg gaaacuuccu guaugaaggc      540

gccacauuua auucuaacgc uuucaccuuu gaggaugugc ucaaacaagc ccugguuaau      600

auuacguuca acugcuucga cacaaauucc gcuaucagcu cuauuuccaa cauccugaug      660

gaaagcggca agaccaaguc ugacaaagcc aaggcgauug agcgccucgu ggauacauac      720

accgucuuug acgaagugaa uaccccggau aaaccccaga aggagcaggu uaaagaggac      780

aagaaaacac ugaaggccuu cgcuaaccuu gugcuggggc ucuccgccaa ucugaucgau      840

cuuuuuggaa gcgucgaaga cauugaugac gaucugaaga aacuccagau cgugggcgac      900

acguaugaug agaagagaga cgaacuggcu aaaguguggg gagacgagau ccauauuauc      960

gaugacugua aguccgucua cgaugccauu auccugaugu cuauuaaaga accugggcuc     1020

accaucagcc aauccaaggu uaaggcguuc gacaaacaca aggaggaucu ggugauccuu     1080

aaaagccugc ucaagcugga ccggaacgug uauaaugaga uguuuaaauc ugauaagaag     1140

ggccuucaca acuacgucca uuauauuaaa caggggcgca cagaagagac cuccugcagc     1200

agagaagacu ucuacaagua caccaaaaag aucguggagg gacuggccga uucuaaagac     1260

aaggaauaua uucucaacga gaucgagcug cagacacugc ucccccugca gcggauuaag     1320

gauaauggcg uuaucccuua ccaacuucac cuggaggagc ucaaagugau ccuggacaag     1380

uguggcccca aauuuccuuu ccugcauacg gucagcgaug gguuuuccgu gacagaaaaa     1440

cuuauuaaaa ugcucgaguu cagaaucccg uauuacguug gaccccugaa cacccaccau     1500

aauauugaca acggcggguu uucuugggcu gugcgcaagc aggccggacg ggucaccccu     1560

uggaauuucg aggaaaagau cgacagagaa aagagcgcug ccgccuuuau uaagaaccug     1620

acaaauaaau gcaccuaucu cuucggcgaa gaugugcugc ccaaguccag ccuucuguac     1680

ucugaguuca ugcuccugaa cgaacugaau aacguccgca ucgacgggaa agcucuugcg     1740

cagggaguga agcaacaccu cauugauucc aucuuuaaac aggaccacaa gaagaugaca     1800

aagaaucgga ucgagcuguu ccugaaggau aacaauuaua uuacgaaaaa gcauaaaccu     1860

gagaucaccg gccucgacgg cgaaauuaag aacgaucuga caagcuacag agacaugguu     1920

cgcauccuug ggaauaacuu ugaugugucu auggccgagg acauuaucac cgauaucaca     1980

auuuucggag aauccaagaa aaugcugcgg cagacccuca gaaacaaguu uggcagccag     2040

cugaaugacg agacgaucaa aaagcugucc aaacuucgcu accgggauug ggggagacuc     2100

ucuaagaagc ugcugaaagg aauugacggc ugugacaagg cugggaacgg cgcccccaaa     2160

accaucauug aacucaugcg caaugauagc uauaaccuga uggagauccu uggagacaag     2220

uucuccuuua uggagugcau cgaagaggaa aaugccaaac uggcucaagg ccaggucgug     2280

aacccgcacg auauuaucga cgagcucgcc cugucuccug cgguuaagcg ggcugugugg     2340

caggcccuga gaauugucga ugaaguggcc cauaucaaaa aggcccuucc cagccggauu     2400

uucguggagg uugcucgcac aaauaagucc gagaaaaaga aaaaggacag cagacagaaa     2460

cggcucucug aucuguacuc cgcuaucaag aaggacgaug uccugcaaag cgggcuccag     2520

gacaaagaau uuggagcccu gaagucuggc cuugcgaacu augaugacgc cgcucugaga     2580

uccaaaaagc ucuaccugua uuacacccag auggggcgcu gugccuauac aggaaauauc     2640

auugaccuga accagcuuaa uaccgauaac uacgacaucg aucacauuua cccucggagc     2700

cugacgaaag acgauucuuu cgacaaucuc gugcugugcg agagaacagc uaacgccaaa     2760

aagagcgaua ucuaucccau ugacaaucgc auccaaacca aacagaagcc cuuuugggcg     2820

uuccucaaac accagggccu gauuuccgaa agaaaguacg agcggcuuac acgcaucgcc     2880

ccucugaccg cugaugaccu cagcggguuu aucgccagac agcuggugga gaccaaccaa     2940

ucugucaaag cuacaacgac ccugcuucgg cgccuguauc cggauauuga cgugguuuuc     3000

gugaaggccg aaaacgucuc cgauuucaga cauaauaaca auuuuaucaa ggugcggagc     3060

cucaaccacc aucaccaugc caaagacgcu uaccugaaua uuguuguggg caacgucuau     3120

cacgagaagu ucacacgcaa uuuuagacuc uucuuuaaaa agaacggagc gaaucggacc     3180

uacaaccugg ccaaaauguu caauuaugac gugaucugua caaacgcuca ggauggcaag     3240

gccugggacg uuaagaccuc caugaauacg gugaaaaaga ugauggccuc uaacgauguc     3300

cgcgugacaa gacggcuucu ggaacagagc ggggcucucg ccgacgcgac cauuuacaag     3360

gcuuccgugg ccgccaaggc uaaagaugga gccuacaucg gcaugaagac caaauauucu     3420

gucuuugcgg acguuacaaa guacggggga augaccaaga ucaaaaaugc uuauagcauu     3480

aucgugcaau acacaggcaa gaaaggggag gaaauuaagg agaucgugcc ccugccuauu     3540

uaucugauca acagaaacgc cacggauauc gagcuuauug acuacgucaa auccgugauc     3600

cccaaggcca aggauauuuc uaucaaauau cgcaagcugu gcauuaauca gcucguuaaa     3660

gugaacggcu ucuacuacua ucugggaggc aagaccaaug acaaaaucua cauugauaac     3720

gcuaucgaac ucgucgugcc ucacgacauc gccacauaua uuaagcugcu ugauaaguac     3780

gaccugcugc ggaaagagaa uaagacccuc aaagcuagcu ccaucacaac cagcauuuau     3840

aacaucaaua cgucuaccgu ugugucccug agcaacaagg ucgggauuga cguguuugau     3900

uacuucaugu cuaaacuuag aacaccccug uauaugaaga ugaagggaaa uaaaguugac     3960

gaacucucca gcaccggccg cucuaaguuu aucaaaauga cacuggagga acaguccauc     4020

uaccuccugg aggugcuuaa ccugcugacc aauagcaaga caaccuucga ugucaaaccg     4080

cucgggauua cgggaucccg gagcacaauc ggcgugaaga uucauaaccu ggacgaguuc     4140

aaaaucauua augaaucuau caccgggcuu uacagcaacg aggugaccau cgucuga        4197


<210>  4446
<211>  4200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4446
augaagacca uccugggccu cgaccugggg acaacgagca uuggaugggc ccuugugaaa       60

gaggcugaac ccuucuccga ugagaagucu gaaaucguca gacugggcgu ucgggugauu      120

ccucucaccg uggacgagcg caccagcuuu gaaaacggga aauccaucac aacaaaugcc      180

cugagaaccc agaagcgggg aaugcgcaga aaccuucaac gguacaaacu gcgcagacag      240

gcgcucauug aggagcugaa gucuacaggc uggaucggga augauuucau cuuuagcgaa      300

accggaccgg gcuccacguu ccggacccug cgccucagag cuaagucugc cagcgaggaa      360

gucacacugg gcgaccuugc ccggauucug cuccagauca acaaaaagcg cggguauaaa      420

uccagcagaa agaugguguc uuccgaugac ggacaggcua uugauggcau ggccguugcu      480

aaagagaugu acgacaaggg gcugaccccc ggagaguaug uguaccaacg gaugcuugaa      540

ggcaaguaug ccaucccuga guuuuacaaa agcgaucugc aggaagaguu cgaccgcauu      600

ugggcgcucc agaagucuuu ucacaucgaa cugacagaug agcuguauag aaaacucucc      660

gaccggaaua agagccagac cugggccauu ugcagagaug agcuggggau ccaaggacuu      720

aaaucuuccu ucaucggcaa ggaacgccug aaagaggcuu accgguacag acaucucgcc      780

gcuacgcagg aagucaacau ggaggugcug acaugugccc uucaggaaau uaauggccag      840

aaggcgagcc uguccgggcu ccugucuaag aucagcgacc gcuccaaaga gcuguaucuc      900

aacaaucuga ccguuggaca aaugcuucug aagcggauug aggacaaccc caauaacagc      960

cucaaaaaua ucguguuuua cagacaggau uaucuggacg aauuugagcg cauuuggaca     1020

guccagucua aguaccacac cgaacuuacc caugagcuga aaaagcggcu cagagauauc     1080

aucauuuucu aucaacgccc ucugaagucc cagaaaggcc ugaucagccu cugcgaacug     1140

gagacaaagg acauugaggu ggccaucgau ggggugaaaa agaugaaaaa cauuggaccc     1200

cgggucuguc cuagaucuag cccgcuuuuc caggaauuua agaucuggca gacgaucaau     1260

aacauuuucg uuaacggcgc ugcccuggac gaugaggaca aguccgugau cgcuaaagaa     1320

cucauguuca aggauaaacu gaaggacucu gaucugcuua aacuccuguu uaagaccaca     1380

aaggggcaaa gcgugaauuu caaagagguc gacggaaacc gcaccauggc cacacugaug     1440

aaggccugcu uuaaaauugc ugagaaucuc ggcuacgacg auaucggccu guccaaacuu     1500

agcucugaag accagauggu gcggauugag gggaucuuca aggaacaggg auuuaagacc     1560

ggcuuccugc acuuugauuc cagccucgcg ucucccgagu ucgaaaacca ggacuccuau     1620

agacuguggc aucugcuuua cagcuacgag ggggauaaau ccgccucugg aaaugagagc     1680

cucguuaagu guauuaugga ccugugcucc auggaugaag gcucugcuaa gauccuggcc     1740

agcuguacgu uucucccuga cuaugggucc cugagcgcca aagcuaugcg gaagauccuu     1800

cccuacauga aagauggccu ggaguauaca gccgcgugcg cuauggccgg auaccgccac     1860

ucuaagagau cccucaccaa agaggaacug gacaccaagg aauauagcga ucggcugucu     1920

cuucucccua agaacucccu gagaaauccc guggucgaga aaauucugaa ccaaaugauc     1980

aaugugguua acagcgugau ugacgucuac ggcaagccgg augagauccg cauugaaaug     2040

gcccgggagc ucaaaaaguc ugcuaaagaa agagaggaag cccagcgcga caucgcuagc     2100

uccacagccg agagccagaa ggugaucgag auucugaagg gggaaccuuu cuuucuucag     2160

uuccccacca gaaaugacau cauucgguau cgccuguacc aagagcucgc gcagaacgga     2220

uuuaaaacac uguacucuaa uacguauauc ccuaaggaua aacuguucuc caaggaauuu     2280

gacauugagc acaucauccc ccaggcccuu auuuuuaacg auagcuucgc uaauaaaacc     2340

cucgagucca gacaguguaa ccuggaaaag ggcaaggaga cagccaugga cuacgucaaa     2400

ucuaaguaug augaaaccgg ggugcaaaca uacugcgacc ggguuaacgg acugaucggc     2460

aaagggauua gcaagaccaa agcuaagugg cuccugaugg agggcaaaga uauccccgaa     2520

gacuuuauua aucgcgagcu uagagauucc caguauaucg ccaagaaggc gcgggagauc     2580

cuggaaucuc ucgugaaauu cgucgugccu acgaccggaa gcguuacagc ccgccugaga     2640

gaggacuggc agcuggugaa cgucaugcaa gaacuuaauu gggagaagua cgcucggcug     2700

ggccucaccg gggugcgcga agauaaagac ggaagacgga uuccguuuau ccaggauugg     2760

acaaagagaa acgaccaucg ccaccaugcc auggacgcuc ugaccauugc cuucacgcgg     2820

agacaguuua uccaguaucu caaucaccug aacuccaaaa uugauguggu uucuugggac     2880

aagaaggauc uugaccugcg cgauuacgac cucgaggaua ucaaauucgg caaucugagc     2940

gccggggacc gguacggaau ugucaaggcu cugcaagaua aauuucuuua uaaggacggc     3000

aacgauaaau acagauucgu gcccccuauc ccccuggacg aguuucgccg gcaggcgaag     3060

gaacagcucu ccgacauccu gauuagcuuc aaggccaaaa auaaggugug uacaagaaac     3120

gucaauguga ccaaaaacaa gggcggcgcu aaucgcaaag cccagcucac accucgggga     3180

ccccugcaca acgagaccau cuaugggucu ucccuugaau acguuaccaa ggagaaugaa     3240

aagauuggga gcucuuuuga uacagagaga aucacgaccg ugugcaaaaa gaaauuccgg     3300

gacgcccugg cucgcagacu cgaggaguuc ggaggcgauc cgaagaaagc cuuuacaggg     3360

aagaacuccc cugaaaagaa ccccauuugg gucgacgaac aucacagcga gcaagugccu     3420

gcgaaaguuc ggaccgugac aaugggacag cgcuucaccg gcagaaagcc caucgaugcu     3480

acgcugaaaa ucgagaaggu cauugacaaa cggaucagag aaauucugca ggcccgccuu     3540

gaugaguuug acgggaaggc cgcuaaagcc uucucuaauc uggaugaaaa cccuaucugg     3600

cucaauaagg agaagggcau ugcgaucaaa cgggugacag uuuccggacc cgcuaacccg     3660

gugccuguca gauuuaagcg cgacaaagau ggcaagccca ucauugacga ugccgggaaa     3720

accaucggag ccgacuucgu gacccccggc aauaaccauc acauugcuau cuuucgggac     3780

agcuccggga agcugcagga acauccugug agcuuccucg aggccacaau ugcuaagucu     3840

cacggacugg augucaucga cagaaauuau aacaaagaug agggcuggga auuucuuuuc     3900

acccugaagc aaaaugagua cuuuguuuuc ccgaacagcg aaacaggcuu caauccccug     3960

gacuaugauc ucacggaccc ucgcaacuac gccgagauuu cccccaaucu guaccgggug     4020

cagucuauca gcaccaacga uuauuucuuc agacaccauc uugaaacaac cuccgagaaa     4080

aauaacucuc uguacgggau cacauggaag cgcauuagaa acgcgagcgc ccucgaggga     4140

cuggugaaag uccggaucga caaucucggc cgcauugugg cuguugggga auaugauuga     4200


<210>  4447
<211>  4200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4447
auggagaccc aggccacagc ucaacugauc agcggccacc ucaaggggua ccccauuuau       60

gacuacuucg ugggacugga uaucggcacg aacuccgucg ggugggccgu uaccaauaga      120

auuuaugaac uucugaaauu ucggucucau aagauguggg gaagccgccu cuucgaggaa      180

ggccagacag cgguggugag acggggguuu cgcuccauca gacggcgccu ggagagacgg      240

cgccuuagac ugaaacuccu ggaagcucug uucgccgacg ccauggcucg ggucgauucu      300

accuuuuuca uucgccucag agagagcaag uaccacuaug aggacaaaac aaccggacau      360

aaguccaagc acauccuguu uaucgaugac aacuacacag aucaggacua uuucgccgaa      420

uacccuacca uuuaccaucu ucgggcugau cugauggaga auggcacguc ugacauccgc      480

aaacucuuuc uggccgugca ccacauucuu aaguauagag gcaacuuccu guacgaaggg      540

gcgaccuuug auagcaauuc cagccucgac acaguucuga aacgggcccu ggagcagauc      600

gaguuccaua uuuuuaacgc uaccucugaa gaugcccucc acuccauccu ggacauugug      660

aaacuucaag acaagacaaa gagcgauaag gcuaaagcca ucgagucucu ggucgacacc      720

uauaucacgg auaaggugga auccagcugc acagacaaag agaagaaaga ucuccugaag      780

gcggacaaaa agagacuucg cgccuucgcu aaucugauuc ucggacugaa cgccaaucug      840

accgaucucu ucggcacaau cgaagagauu gaggaagacu uuguuaagcu gcagaucacc      900

ggggauaccu acgaggaaaa acgggacgau uaugcuaagg uguggggaga cacaauuaga      960

aucaucgacg auuguaaauc ugucuacgac uccauuaucc uuaugagcau uaaggagccg     1020

ggccugacga ucucccaguc uaaagugaag gccuucaaca agcauaaaga agaucucgcc     1080

auucugaagc gccuucugaa aagcgacaag uauaucuaca aaaauauguu uaaggcugau     1140

gagaaggggc ucgcgaacua cgugcacuau aucaaacagg gacggaagaa ugagaccggc     1200

ugcuccugug aagacuucua caaauauaca aagaaagucg uugagagccu gcccgauucu     1260

uccgacaagg ccuacauucu gaacgaaauc gagcuccaaa agcugcuucc ucugcagaga     1320

auuaaagaua auggcgugau ccccuaucag cuccaccugg aagaacugaa ggcuauucuu     1380

gagaaaugca aggacaccuu uccuuuccuc aacacaguga gcgauggguu uaccgucgcc     1440

gaaaaacuga ucaagaugcu ggaguuccgc auucccuacu augugggacc ucucaacccg     1500

ucucauagca ucgacaaagg cggguuugcc uggguugaac ggaaggagcc cggacaggug     1560

acgccuugga auuucgagga uaagaucgac agagaaaaau ccgcugaggc cuuuauuaag     1620

aaccugacaa auaaauguac cuaccuuauc ggcgaagacg uccugcccaa gucuagccuc     1680

cuguacuccg aguucaugcu gcuuaacgaa cucaauaacc ugcgcauuga ugggaaaccu     1740

cuggagcaag cggugaagau gcaccucauc gacgcuguuu uuaagaaaga gcauaagaaa     1800

cagaccaaga aucggauuau cgaguuccug aaagauaaca auuauauccu uggaaagcac     1860

aagcccgagg uggacggccu ggaugaaagc auuaaaggcg accucgccuc uuacagagau     1920

augaagcgca uccuggggga cgauuucgac gucuccaugg ccgagagcau uaucacaucu     1980

auuaccaucu uuggagaauc caaaaacaug cugcgggaga cacuuagaaa gaaauucggc     2040

agcauccucu ccgaggauca gauucggucu cuggcuaagc ugcgcuauag agacuggggg     2100

cggcucagca agaaauuucu gaucggaauu gauggcugcg ugaaggggga agacggcacg     2160

ccgggaacca ucauugacau gaugagaaau acaucccaua accuuaugga gcugcucggc     2220

gauaccuucu cuuuuaugga cgucaucauc gaagagaaug auaaacugac aaaggggcaa     2280

gugguugacc cucacgaaau uaucgaggag cugggacuua gccccgccgu gaaacgcgcu     2340

gucuggcagg cccuccggau uguggaugaa guuauccaca uuaagaaagc gcugccuucc     2400

agaaucuucg uggaggucgc ccgcaccaac aaggacgaaa agaaaaagaa agauagcaga     2460

aagaaacggc ugcaggagcu cuacaagucu auucagaagg agucccccga auggcugagc     2520

gugacgcgcg acgagucuga uaaacuuauc uccaaucugg acagagcuga ugacagcucu     2580

cuccggagca agaaacugua ucuguacuau acccaaaugg gccgcuguau guacuccggg     2640

agagugaucg accuugauca gcugaacaca gacaaguacg auauugacca uaucuauccc     2700

cggagccuca ccaaagauga cucuuuugau aaccugguuc ucugcgaacg ccaggagaau     2760

gccaagaagu ccgacaacua cccuauugau ccggacaucc agaaugcuag aaaacccuuc     2820

uggacauuuc ugaaggauca aaaccuuauu agcucccgga aauaugaacg ccugaccaga     2880

guccucccuc uggaggccga cgaucugacg ggauucaucg cgcggcagcu uguggagaca     2940

aaucagucug ugaaggccgu caccacacug cucagacgcc uguaccccgg cguggacguu     3000

guguuuguca aagcugaaaa cgugaccgac uuccggcacg auaaugacuu uaucaagguu     3060

agaagccuca accaucacca ccaugccaag gaugcuuauc ugaauauugu ggucgggaac     3120

guguaccacg agaaauucac ccgcaauuuc cggaaguuuc uuaaaaagaa cggcgccaaa     3180

agaacauaua aucuggcccg cauguucgac uuugauguua uguccacgaa cgcuaaggac     3240

ggaaaggcgu gggauaucaa aaccucuaug aauauuguga agaaaaugau ggccagcaac     3300

gacguccggg ugacaaagag acucauggaa cagaaaggcg cucuguccga ugccaccgug     3360

uacaaggccu cugucgcugg gaaagccaag ggaggcgcgu acaucgggau gaagacaaaa     3420

gacagcguuu ucuccgaugu gagcaaguau ggaggccgca ccucuauuaa aaacgcuuac     3480

uccaucgugg ucgaguauac ggggaagaaa aaugaaauca uuaaggagau cgugccucug     3540

cccguuuacc uuauuaaccg gaauaagaca gaccaagagc ugauccauua uauugugagc     3600

aucauuccgc aggccaaaaa cgucucuauc aucuacaaga aacucuguau uaaucagcug     3660

gugaagguua acggcuuuua uuacuaccuc ggaggcaaaa ccaauaacua uuucuggauc     3720

gauuccgcca uucagguggu caucgcugac gaauggauuc cuuaccugaa ggugcuugag     3780

aaguuugacg uuaugagaaa acaaaauaag gaacugaaag uggggaccgu cagcacaucu     3840

auccagaacc agggaaagaa aguggugauc aagauucugg aggaacggaa ugucgaguuc     3900

uuuuccuauc ucguuggcaa gcugcacagc uccaucuaca gcaaaaugaa ggggaacaaa     3960

gccggagagc uugcgaccaa gucucuggaa auuuucguga auaugagccu cgagaaccaa     4020

ugcaucuauc ugauggaacu ccugaaucuu cugacaaaca aaaaggcucc cgaugugaaa     4080

ccucugggca uuacggccuc ccgcgggacc aucggcucua aggucagcaa ccaggacgag     4140

uuuaagauca uuaaugaguc caucacagga cucuacucua acgaagugac cauuguuuga     4200


<210>  4448
<211>  4200
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4448
augaaccuga gaaauaccac agacgaguac aacaucggcc ucgauauggg gacgaauagc       60

gugggauggg ccgucaccga caaggcuggc gccauugcgc acuucaaggg gaagcccaca      120

cugggauccc ggcuuuuuga ugcugcccag accgccgcug gcgcccgcau ccauagaucu      180

caacggcgca gauauguucg gcgcagaugg cggcuggacc uccugcagag ccuuuuccag      240

gaugaagugu ccaaagugga cucugaguuu uucugccgcc ugagacagag ccggcucuac      300

gucggggauc gcgaaaaggg acacgcugac uauacauggc cuauuuuuaa cgauuccgac      360

uucaaugagg uggauuacua uaaaagauuu ccgaccaucu accaucugcg gaaguggcug      420

auggaaacag acgagaaggc cgauauucgc cucaucuauc uggcgaccca caacaucguu      480

aaacauagag gcaauuuccu ucgggagaac aauaagucuc ugagcuccag agacgccaac      540

cccgcucaag ccgugaaaag ccuccgccug gcucuuaaug acugguguuc ugaacgggau      600

uacgagugcg ccuggaaggg cgaagaggac gauggggcga uugacgaggc cauccugaac      660

guccucgcug auggagaaaa aggcagaucc gaccaggcca aggagauugc uaagcugacg      720

gggguggauu guggagacgc caaacagagc aagcagugca augaagccau cgcuaaagcg      780

auuguuggcc ugaaggugga gaugaaaaac aucuuugggg aguucccugc cgaaaagacc      840

ucuuacauuu aucucuccag cgaugaggac gucgaagcug ugcgcgaguc uugucccgau      900

gacuccggag aacuguuuca agcccuuugc aaacuguaca gcgccuauau ccuccaggag      960

cugcuuuccu acgcugaccc uucuggccag acaaucagcg ccaauauggu ggcgaaguau     1020

aagcaguacg gccgggaucu gagacuccug aaagaacuga cccgcacaua ugcuccccaa     1080

aaguacgacc ucuucuuuag aggggagacc uacgccggau ccagcgauua ugucguugac     1140

gaggccagag gcuacacgcg cuauaaccug gggacaacca aagcuaacua cgaugacuuc     1200

cagaagucug ugaaagaacu uuucaaggga acagaugccc ugcaggacga gcgguaucaa     1260

gaaaugaugg uggcuuuugg cgagcagaag uuccucagac gccugaaaac cuccgauaau     1320

gggagcauuu acuaucagcu ucaccuggag gaacuccaca ccauccugga gaaccaggga     1380

aagcauuacc cuuuucugcu cucugaagag gacaaaauug aaagccuggu cuccuuccgg     1440

aucccguacu augugggccc ccuuacaaga aagaaugccg cgauuggcca cgaugggaaa     1500

gagcgguuug ccuggucuag ccgcaaggac ggaauggagc augcuguuau cacgccuugg     1560

aacugggaag agaucauuga uagagacaag uccgccgaag acuucaucau gcggaugacc     1620

ggcgauugua cauaccugca aggggagccc gugcucccua aaugcagccu gcuguaugaa     1680

gaguuuugug cucuuaauga guucaacgga gcccacugga ccgucgacgg cgaugacgaa     1740

agacgcuuug augcggccca gcgggagaga auuaugugcg accucuuccg cagaacacgg     1800

ucugugaccu acaauaaggu uuccgauugg cuggaacgca aauauaacuu uacgggggcu     1860

agagugcggg gcggacaggg cgagcacggg uucgaaagca agcugggauc uuacaucuuu     1920

uucuguaaag acgucuucaa gacagauggc cucgacgagg ccgauuauac caugauugag     1980

cagaucaucc ugugguccac ccuuuuugaa gaccgcgaua uucugaagag aaaaaucgag     2040

gaggcuuacg ggggagaccg gcucacaagc gaacaaauua aggugaucug caaaaagcgc     2100

uucaccggcu gggggagacu guccaaacgg cugcuuacag acauuaaggu caauacggau     2160

accggccgca aaucuaucau ggacgugcuc agagaaggaa accccaauaa cggccagcgg     2220

agcagaacaa ugguuuuuau ggaggugcug cgcgaugagg accugggguu ccagaaggcc     2280

auugauggac ucaaucggga cuacuuuaga gaacauaaca auggccugau ggaugucaac     2340

gaccuuccgg gguccccugc cauccgccgg ucuaucaacc aggcugugag aauuguugau     2400

gagaucgcga gcauugcccg caaggaaccc accaauaucu ucauugaggu gacacgggag     2460

gaagacgauc cucggaaaaa gggaaaacgc accaagcgga gauaugacgc uaucaaaucc     2520

gcccuggaga aguuuaagaa agacgauccc agccucugga aggaacugug cgagaaagcc     2580

cccggcgaca uggaugaacg gcugucucuu uacuucaugc aacgcgggaa guguauguau     2640

uccggcagac cuaucaaaau ugaggagcuc cacacgggaa aguacgaagu cgaccauauc     2700

auuccgcgga gcuauaucaa ggaugacucu uuugagaaca aagugcuggu guaccgcgaa     2760

gagaaucagc acaagaccga ugcucugcuc auugacucca gcaucagaca gaaaaugggc     2820

ggguauuggc ggaugcugca ugaugccaaa cuuguuggag acaaaaaguu cagaaaccug     2880

uuucgcaauc acaucgauga caaggcgaug aaaggcuucg ucgcucggca gauuguggaa     2940

acaucucaaa uggugaagcu cguccagacc cuguuugagg ccagauaccc caacacacgc     3000

aucgugccug uuaaagccgg gauuagccac gaucugcggg aggcugccgg acuugugaag     3060

ugcagagaag cgaaugacuu ccaucacgcu caugacgccu uccucgccug ucgcaucggc     3120

cuguuuauuc agaaacggca ccccuccguc uacgauaacc cuaucgggcu ggcuagaauc     3180

augcgcgacu augccagaau gcaagcucgg gaguacaaga aaagccacug ccucgcccau     3240

acccagggcu ucauuguuaa uucuuuucug uccagcggau ucgaugaaga gacgggcgaa     3300

aucuuuaagg acgauuggga cgcgaaggcc gagguggagg ggauucgcag agugcuuaac     3360

ugucggcagu gcuucaucuc ccgcaugccc gucgaaggag auacaagaua uggcgggcgg     3420

uacuggggag agaccauuua uucuccgaaa ggcaagcaca aacaugaaau cccucugaag     3480

cagggccucg accccaaucu guacggggga uauagcgauc ugcuuuacuc cuacuuucug     3540

auuuaugagg cuucugacgu gagcuccggc gagagaguuc uccgcuucau cgggaucccu     3600

acaucuauug ccgcucggau ggccaaccaa aagagacugg aagaguacgu gagccgccuc     3660

gcccugcagg aaaaucuuga gcacguccgg auggugagaa agaucaugaa gaaacagcug     3720

auugaagugg auaacugucg gcuccugguc accgcugcgg acgccguuac caaugcuaca     3780

gagcuggccu uuucccuuga ugagaugaag aucauuagca cgugcgccaa auguaucaac     3840

ggagcugacc ccucugccau cgacaagauu ggcaacgcgc aggauuccuu cuuugacaau     3900

cugaaacgca gagggagcga ucggugcgcu cuccuguucu cucaacucaa gcuggacaaa     3960

cuuucccaga acuucuauau gaucagcucu gaugacaagu ccaagauucu gaucagcauu     4020

cucgaucgca ucagagccaa auccaauagc guggaccugu cucugguggg aggcgccaag     4080

caugcugccc agaugaaauu ugauuucaac gcgaaacuua auaacccugc uagcuccuuu     4140

guccugaucg accagucugu gaccggcaug uucgaacggc gcacaagaau ugggcucuga     4200


<210>  4449
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4449
augaaccuga gaaaugccac cggcgacuac agcaucgggc ucgaucuggg aacaaacucc       60

gugggcuggg cugucacgga cucuaauggg agcauggccc acuucaaggg aaaacccacc      120

cuuggcuccc ggcuguuuga ugcggcugag acagccgccg gggcucgcca gcauagagga      180

caacggcgca gauauguucg gcgcagaugg cggcucgacc ugcuucaggg ccuguuccag      240

gaugaaguga acaaggugga cucugaguuu uucauucgcc ucagacagag ccggcugcac      300

gucgaagauc gcgccgaggg ccaugcuggg uacagauggc cucuguuuaa ugacaccgau      360

uucacagaaa aagacuauua caagcgguuu ccgaccaucu aucaccuccg caaauggcug      420

auggagacag augagaaggc cgacauuaga cuuaucuacc uggcggccca uaacauugug      480

aagcaccggg gaaauuuccu cagagaagau aacaaaaauc uguccucuca caacgcuaau      540

cccgccgagg cuguucgcag ccuucggcaa gcccuggacg acuggugcgc ggaaagaggc      600

uaugugugug ccuggaccuc cgucgaugac cucgcugagg ccgauagcca uauccuggac      660

auccugacga acucuaagga gcgccgggcu gaucaggcca aagaacucgc caagcuguuu      720

gggguggacu gcggagauac caaacagucc aagagcugua auucugcucu ugcgaaggcc      780

guugugggcc ugaaaacaga gcucaaggac gucuucgggg aauuuugcgc ugagaaaacc      840

gccauucacc uguccagcga ugacgaugug gaagccauca gagacgcuau uccugacgau      900

ucuggagagc uuuuccaggu gcugugugcc gucuacuccg cguacguucu ccaagagcug      960

cugagcuaug cucccggcca gacaaucucc gccaacaugg uggccaagua cgaccaguau     1020

ggcaaagauc ucaagcugcu uaaaaaucug gcucgcaccu acgugucucg ggaaaacuau     1080

gacgccuuuu ucagagggca gacguacgag gauagcaacg acuauaaugu cgauaaggcu     1140

caaggauaca cacgcuacaa ccucggcacc acaaaggccu ccuaugacga uuucaaaaag     1200

agcguggaaa aacuguuuaa ggggacccag gcggagcagg acaaacggua ccaaggaaug     1260

augaagagau ucgccgaaga gaaguuucuu cgccggcuga aaaccucuga uaauggcucc     1320

auuuauuacc agcuccaucu ggaggaacug agagcuaucc ucaagaacca ggggaaacac     1380

cacccuuucc ugcuugagga agagaagaaa auugaaagcc ugguuucuuu ucggaucccc     1440

uauuacgugg gaccucucag cuccaagaau gccucucugg accauaaggg caaacagcgc     1500

uucgcuugga gcgagagacg gccgggcaug aaggauacag ucaucacgcc cuggaacugg     1560

gaccaaauua ucgacugcga ucaguccgcc gagaaauuua uucugagaau gaccggggac     1620

uguacauauc uucagggaga accugugcuc cccaagugca gccugcugua cgaggaauac     1680

uguguucuca augagcugaa cggcgcgcgc uggaccaucg auggggacga uuggcggaga     1740

uucgacgugg cccagcgcga gggcauucuu cacgaucugu uuagaaaaac acggucuguc     1800

gcuuauaaua agguggccga cuggcucgaa cgcgaggaaa acugggcuaa ugcccauguc     1860

agaggagggc aaggcgagaa cuccuucgaa agcaagcugg gaucuuacau cuuuuucugc     1920

aaagaugugu uuaagaccgc cgcucuggac gaggcggauu auccuaugau cgagcagauu     1980

auccuuugga auacacucuu cgaagaccgg gccauucuga aacgcaagau cgagcaggcu     2040

uacggcgaac accauggggg aaaacugacg gaugagcaga uuaagguuau cuguaaaaag     2100

agauucaccg gcugggggcg gcucucccgc aagcugcuua ccggaaucaa aguggacgcc     2160

gacacaggca gagucagcau uauggaugug cugcgggaag ggaaccccaa ugagggccaa     2220

cgcuccagaa ccaugguucu cauggaggug cugcgggacg aagaucuggg auuucagaag     2280

aaaaucgacg auuucaacaa ggccuauuuu gcuggccagg gggagggacu uaugucuguc     2340

aacgaccucc cgggcagccc ugccauuaga cgcuccauca aucaggcgau ucggaucgug     2400

gaugaaauug cuucuaucgc cgggaaagcc cccagcaacg uguucaucga gguuacaaga     2460

gaagaggacg auccucgcaa gaagggacgg agaacgaauc gccgguacga ggacauuaaa     2520

aacgcucugg aaaauuuuaa gaccgaggau caagaacugu gguccgagcu ccugggcaaa     2580

gcccccaacg accuugacga gagacugagc cucuauuuca ugcagcgcgg gaagugcaug     2640

uacucuggcc ggcccaucaa acuggaagaa cugcacacag gaaaguacga agucgaucac     2700

auuaucccua gauccuauau uaaggacgau agcuuugaga auaaagugcu ugugugccgg     2760

gaagagaacc agcgcaagac caaugagaug cucaucgacc cggcgaucag acggcgcaug     2820

ggcgauuacu ggagaaugcu gcauaaagcu aagcugauug gggacaaaaa guucaacaau     2880

cucuuuaugc gggauagaau cgacgauaag acacugaaag gauucgucgc ccgccaacuu     2940

guggaaaccu cucaguccgu uaagcuggug cagacgcucc uggagguccg guaucccaac     3000

accaaaauug ugccuguuaa ggccaauauc agccacgacc ugagagaagc ugccgaucuu     3060

gugaaauguc gcgaggcuaa cgacuuucau cacgcccacg augcguaccu ggccugccgg     3120

gucggccucu ucauucagaa gagacauccc ucuguguaug cuaauccuau cgggcugagc     3180

cgcguuaugc gggacuacgc cagaaaacaa gcuggagaau uuaacaagac acacuccaug     3240

gcccauacca agggcuucau cgugaacagc uucaugacau cugaguuuga caaagagacc     3300

ggggaaauuu ucugggaugc cgaggcugcg gucgaagaca uccgcagagu gcucaauuau     3360

cggcaguguu uuauuucccg caugcccgug gaggauacgg gcgccuucug ggaccagaca     3420

aucuacagcc cgagauccaa caagcggcug gaacuuccuc ugaaagauga ccucgauccc     3480

aauaaguacg gaucuuauag ccgcgagcag uuugcuuacu ucuuuauuua ugaggccaga     3540

gacgccaaaa aguccaagac cguccugcgg uucgcucaag uuccugugag agugacaucu     3600

gccaucgcgc gcgauagcuc caaacuggac gaauacgcuu cuggccuugc cguccaggag     3660

gggcuggaau uuaccaagau cauucggaga cgcauccuca aaaagcagcu gcucgagcug     3720

aacggagauc ggcuugugau uaccggcgcc aaagagguua gaaaugcuac agaguucgcc     3780

uuuacgcugg aggaaaccgc gcucguggcu augugcagcg agaucgcccg ggaagggguc     3840

cccuccgaga uugccagcga cgugcagucu aucuccagcc aacugucuga ucuguuccuu     3900

gcucugauug agcgcggacu cacaaucggc guuagacugc acggccagcu caagcugggg     3960

caguaucuuc ggcaauucga agacgccgcu cugcgcgaca aggccaaagu gaucauugga     4020

aucauuucca ucaccaacgg cgcgacaaau acccuggauc ucagcucucu gaacgggucc     4080

aagaaugucg gacaggugag aauuacgcau aacaaacuuc ugaaugacaa gagcacaucc     4140

uacguucuca ucgaucagag cgugaccggc auguuugagc ggcgcaccag aaucgggcug     4200

uga                                                                   4203


<210>  4450
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4450
augaagacca ucacagagga cuacauucug ggccucgaua ucgggacgaa cagcugcgga       60

ugggccguga uggacaaaaa gaauagacug cuucggcugc gcggcaaaag agcuauuggg      120

ucccaccucu ucgaagaggg acauucugcc gcggaucggc gcagcuuuag aaccacacgg      180

cgcagacuga agcggcgcaa auggagacuu cggcugcucg aagaguucuu ugcugaaccc      240

auggccaagg ucgacccugc cuucuuuguu cgccugcacc aguccugggu gucuccgcug      300

gauaaggaca gaaagaagua ucaugcuauc guguucccca ccgccaaaga ggaucacgag      360

uuuuaccaug acuauccuac aauuuaccac cuccgggaug cucugaugcg cgaagacaga      420

caauucgauc uucgggagau cuuucuggcc auccaccaua uugucaagua uagaggcaac      480

uuccuccagg acacccccgu gaauagcuuu guugcgucca aaaucgaugu ggccucuuuc      540

cugacaaacg ucaaucaggc uuacgccgac aaguaugggg aggagcagaa cuuuauugag      600

cuuaaugaaa acaaugcugc cgcgaucgag gccgugauuc gcggaaagga caaagauaag      660

accacgaaac aacuggacaa ggcuaaaaag aucaccaaac uccugcagag cuccacaaac      720

aaguacgaaa agaauguuac ccggcagauu gccaacgcua ucaucggcua caaaacacag      780

uucgagacca uucugggcaa ggauaucgac aaaaaugaua aggccgacug ggaguucaaa      840

cucagcgaug gggacgccga ugacaagcug gagaaccuuc ugccugaguu ugaugacaau      900

gaccaauaua uuguggcuga aaucaagaaa cucuucggau cuauugcgcu gucccagauc      960

gucuacgaga acaugacgcu uagccagucu augaagugua aauauaagga ucacggcgaa     1020

gacuacgauc ugcucaaaca guauaucgcc acacuguccg accauagcca agugagaaag     1080

cugcuccugg cuuacgaucu uuaugugaac aaucgccacg ggcggcugcu cgaggccaag     1140

aaagaauuua agcaggacaa agcccugacc caggaugagu ucuacaagau ugucaaagag     1200

aaccuugacg auucucaacu ggcucagaag auccuccaga agauugacga aggaaaauuu     1260

augcccaagc agagaguuaa uaaaaacggc uacaucccuu aucaacugca ccagauugag     1320

cuggaucaga ucaucgccaa ucaguccaag uacuauccgu uccucgcgga agagaacccc     1380

gugaaagaac aucgcaagca agcuccuuac aaacuggacg agcuugugcg guuuagaguc     1440

cccuauuacg uggggccucu gauuagcccc gaugacaagc gcaaggacga gcagcagaau     1500

aaaaaccacg aaauguucaa guuugccugg augaaacgga aggaugacaa uggagccauc     1560

acaccgugga acuucgcuca gaaaguugau agagagaccu ccgccaaucg cuuuauuaag     1620

cggaugaccg ugaaggacac auaucucauc ggcgaagaug uccugccugc uagaucucug     1680

cuuuaccaaa aauucgaggu gcucaacgag cugaauaaca uucggguuaa uggcaagcgc     1740

cuggaaccca aauuuaagca gaaaguguuc aacgaacucu ucaagcagaa gaacaaaguc     1800

acgaagaaag ccuuuaagga guucauccug cagaauaccg ggaugaaaac agcggaaauu     1860

gacggacuug ccgauccuac caaguuuaac agcucccuga gcucuuacua ucaucucaag     1920

uccacaaaag uguucgacaa gcaacuggau gacccccagu accaggcuga uuuugaaaag     1980

aucaucgagu auagcucuau uuucgaggac gccgaaaucu accaagagaa gcugcacaaa     2040

gaaauuaagu ggcuuacccc cgcucaguuu aaagccaucg cgaauuggag acuccagggc     2100

ugggggcggc ugagcagaaa gcugcucguc gagcugaagg gaaaaggcgg ggaaaacauu     2160

aucuaucagc uuuggacgac acaaaagaau uucaugcaga ucgugaacga gccugagcug     2220

aaaaaggcca uuguuaaaga aaaucaucag gcuaccgagg aaaagggaau cgagaagauu     2280

cucgaugacu ccuuuaccuc uccggccaac aaaaaggcua uccgccaggu gauuaaaguc     2340

guggacgaug uugugaaggc cgccggcggc aaaaagcccg cucaauucgc gaucgaauuu     2400

acacgggagc cuaaguggaa uccccaccug agcaacauca gagggaccaa acugcuucag     2460

gucuacgagg acacagccaa gaaacucgug gaucaguccc ugacggacag ccugcgcgaa     2520

aauaugacca acagaaaucu ccugaaggau gaguauuucc uuuacuuuau ucagggaggc     2580

cgggacgcuu acacagggaa aaagaucgau auugacgaua ucccuaagaa auaucaaauu     2640

gaccacaucc ugccccaguc uuucaucacc gaugacuccc ucgacaaccg cgugcugaca     2700

gaaauugaga ucaaucagaa gaaaggaaag aaauacgcca gccaucuguu cgccccgaag     2760

ccugcuuuug gccuuaacca aucuauuucc gagaugugga agaccuggca gaaagccggg     2820

cucaucagcc agagaaagcu gaacaaucuc cuguucgauc ccgaccuggu uaaagauaag     2880

cagaaaaagg gcuuuauuaa ccggcaacuu gucgaaacgu cccagaucau uaaacuggug     2940

ucuaucaucc uccaggacaa guucccugau accgagauua ucacagugaa ggcgggauau     3000

aauaccgcuc ugcgcaaaga acugaaacuu uacaaaagca gagaggccaa cgacuaucac     3060

caugccauug augcuuaccu gacagccauc ugcgacaauu uucucuauca ggucuacccc     3120

aacuuccggc cguuuuucgu guacggccaa uuuaagaaau ucucccagga uaccgacaag     3180

cagaagaaag cgguuaagua uuacgggcug aaaaauuuuc uccugccccu ucuggaugac     3240

ucuacgggag augaagugau ggucaaccac acaaagcacg ugguuuucca uaaacagcgc     3300

gacauuuuca auagacucau ccaggcuuau aacuacaagu auaugcuggu gagccgggag     3360

accacaaccg aaaauuccaa cauguuuaau augaccaucu acccgcgcgg cgaaagagac     3420

cggaagaaaa caagaaagcu gauuccuaaa gggaaggauc uugaccccgc caucuaugga     3480

ggcuacagcg ggaacacgga ugccuacaug gucauuguga aaaucgagaa gaccaaggaa     3540

acacuguaua aaguuguggg cguccccaug cgcgcucucg ccucucugaa gaaagcucaa     3600

aaggagggaa auuacgacca ggaacucaac aaagugcugg agccucuuau uauguucaac     3660

aagaauggca aauccaaggc cgggaucaag ggauuuaccg uggucaaaga ucacguucau     3720

uucaaacagg uggugaucga cggcgauagc aaauuuaugc uguauucugc gcaauaccgg     3780

gccaacgcua agcagcucac acugucccag gagaccauga aagucgugac ggacaauaca     3840

gaugacaugg aucaaaagaa cgccgacaag gaucagcugc uucugaaugc uuaugacgaa     3900

auucucgaga aaguugacca guaccugccu cucuucgaua ucaacggguu uagacaggga     3960

cugcacgacg gccgcgccaa guucgccgcu cuuaaaacca gcgaaaagaa auauacccug     4020

aagaaugugc ugaacgggcu ccaugauaau cuggucacac ccaaccuuaa gaauauuggc     4080

aucaaaaccc cuuuuggacu gaugcaaucu agcuccggca uuacacucag ccccaacgcg     4140

aagcuggugu uccagucucc uacggggcuc uuugagaaac ggguuaagcu gaccaaucuu     4200

uga                                                                   4203


<210>  4451
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4451
augggcaagc agaaauucaa cgacuacuau cugggguuug auaucggaac caauagcgcc       60

ggcugggcug ugacagaccu ccaauacaag cugcuuagau ucaacaaaaa ggauaugugg      120

gggucccggc uguuugacga ggcccagacg ucuaaagaac gcagagucua ucggaauagc      180

cgcagacggc ucgaucgcag aaagaagcgg cugaaacuuc uggaggaacu cuuccuggac      240

gagauuucca agaucgauaa caccuuuuuc aaacgccuga aggaaucuaa ucucuacaac      300

gaggacaaaa gcauuaaguu uaauuauccc cuguucaacg auaaggacua cacagauauc      360

gacuuuuaua augaguacaa aaccauuuac caccuuagaa aggaucugau cuucgaagac      420

aaaaaguuug acauccggcu cguuuucauu gcgcugcauc acauccuuaa auaucgcgga      480

cauuuuauuu ucgagggcca guccuucgaa gagaucaagu cuuuugauag cauuuucaca      540

cagcugucca gcuacuuucu cgagaacuuc gacuuuaaua ucgauguguc uauuaaagaa      600

aagcuggagg aaaucauccu guccgacaac accaagagca uuaaaaagac agaguucaaa      660

aagaucuuuu cugauaauaa agugcucucc guccugaaca uggcuauugg gagcaaggug      720

ucucuuaagg acguuuucaa uaacgaggaa cugaaagagc aaaagaucuc cuuuagcgaa      780

auugauuaug agaacgaaaa gagagacgag uucuaccagc uccugggaga agauaucuuu      840

cuuaucgacc uggccaagaa aauuuaugau uucauggugc ucaagaagau ccugaaaaau      900

accgacuccu uuucugccag caaggucgcu cuguacugcg agcacaaaaa ggaccucgau      960

gaccugaaau auauuuucaa gaaguaccuu acgaaagagg auuacuucaa guuuuuccgg     1020

uccaacgugg aaaagaucaa uuauccuagc uacauuggcc ugaacaagau caaaucuaag     1080

aaaaucauua aggagaauaa guguuccauc agcgacauua acaaagaacu caagaauaaa     1140

uaucugaagg auaucaaacu ugaggccaag gacaaggaac uguuuaaccg cauuuucaau     1200

aaacucgaug aguuuaaguu ccuggagaaa cugaagucua gcgaaaacuc caccguuccg     1260

uaccaggugc augagaucga acucaaaaag auucuggaca aucagagaaa guauuacccc     1320

uuucuuaacg agaaaaucga uggcaagucu gucuaugaga aaaucauuca acuguucgaa     1380

uuuaagaucc cuuacuacau ugggccccuc aauacagacc cuaacgugaa aggaaagaau     1440

accugggugg uccggaagga ugagaacaac aaaacaccca ucaccccuug gaauuucgaa     1500

cagaagguug acaugcagaa aagcgcugag gaauuuauua agagaaugac gaaccgcugc     1560

acauaucuga aagccgagga ugugaucgcg aaaaauuccc ugcuuuacga ggaguucauc     1620

cuccugaacg aacugaauaa agugaccguc gacaacgaaa uucuccaccc gacacugaag     1680

accgagauca uuaaugaacu uuuuaagacc acagugaaag uuacgaagaa aaaguucauc     1740

gaguuccuga aaaagaagca ggccgugaac gacgucgaga gcauuaccgg caucggggug     1800

gaauuuaauu guaaacucca aggauauaac acauuccgga agauccuggg caaaaagauu     1860

gagaauguua acgugaaaaa uuuugucgaa aagugcaucg agcugaagug ucuuuacggg     1920

ucugauaaaa agcuguucga aaacgcuauu ggaaaaaucu auagagagua caauauuacc     1980

gacgaggaau uuaacaagau caaccgccuc aauuucaaca aaugggcccg gcuguccaag     2040

aaguuucuca cagagaucag cuucgaaaag accgauggca cgaagagauu ugagucugug     2100

auugaggcuc ugcgcuccac aaauuauaac cuuauggaac ugcucagcga caaauucaau     2160

cugcagucca agaucgauga gauuaacggc gaagacgauu uuucuaauau cgacauuaaa     2220

gaguacgucg auaagcugua cgugagcccc uccguuaagc ggucuaucau ucagaccuau     2280

aaaaucgcca uggaaaucaa gaaaauuacc aagaaagacc cuaagaaaau cuucauugag     2340

auggcgaagg auaaggggga gaaaggaaag acaaccgaga aaagaaagga caaaaucaag     2400

aaacuuuaca aaaacauuaa ggauacacuc agcgacuauu gcaauaaccu gcaagaccug     2460

acgcuccagg uggaaaaaua cgaugacaau cgccugcggc agaagaaacu uuuccuguau     2520

uuuaugcaga ugggcaaguc cauguacacc gggagaugua ucgaucucga agaccuguuc     2580

aacaauaacg ucuaugauau cgaccacauu uacccccaaa gcaaggugaa agaugacucu     2640

uuugauaaua ucguucuggu ggagaaggaa gagaaccauc gcaaauccaa cgagcuucuc     2700

aaggaagaga uucagagccg gaugaauaac uacuggaucu uccugagaga aaauaaauuu     2760

auuucuaagg agaaguauaa ccggcugaca cgcaaaaagg acuucaccga ugaggaacuc     2820

ggagacuuug ucgccagaca gcuggugaca acccggcagu ccacgaaaga gaucgcuaag     2880

uucuuugaga aaaucuucga aaagaccaca gugguuuaca gcaaagccuc ucuugucagc     2940

gaauuuagag agaaguauga cauuaucaag gugcgcgagg ugaaugaugc ucaccaugcc     3000

caagacgccu accugaacau ugucgugggc aauguuuuca acaccaaauu uacaaagaau     3060

uauuggaaau ucaccaagga uaucaaacgg cagaacgaag agaauccuaa cgaaaagaag     3120

ggguacaauc ucagaaaaau uuaugagucc gacguguucu gcaacgauag cgucgcuugg     3180

guguacucua aggaaaaucc caaauacaac acgaucaaua caguuaagga caugguguau     3240

cgcaaaaccg cgaacauuac aagaaugcug gucgagggaa agggccagcu guuuaaccuu     3300

accaucaagg auaaaaccca gcugaagaaa aaugugggca acaucguugc caauacaccg     3360

auuucccuca agaaccggaa acugcaccuc aauagcgugg agaucgcuaa gaaguacggg     3420

uauuucgacu cccugaacaa ugccuacuuu guccuuuuca aauaugaaga gaagggaaaa     3480

gaaaagauug gcuuugauuc uaucacgauu gccuacaaag agcgccugaa gaaaaaccuc     3540

gacaucgaaa aguuccugga gaauaagggg uauaaaaaca gcaagcugau cagaaaaauu     3600

uuuaagaauc aaaaagugcu uaucaacgga uacccuuacc ugcucaaguc cuucaauaag     3660

cagucucaga uuaaauuuaa caauuucaag auccuguauc ucaacgagaa caaucugaaa     3720

uuugcuaagg augccauuaa auucgaaaag aacaaugcga acguggaccu uaagaaacag     3780

aaguacaucg ucagcuuucu gaaaaagaaa aagcaaaaug agaucaacga aaccuaugag     3840

gaauguauug aucggcucaa ugacgaguuc gacacacugu uccugaaacu uugcgauauc     3900

cagaaauccu cuauuuacac caacuauaag cugcaggacu uuagcuccaa uaaaaagagc     3960

aucgagauua aagauaaguu cgacaucuuu aagucucucg aucuguacaa aaaguccaaa     4020

cucauuaagg aguuccugaa cuauuacaaa uacgaaaaga augaccuuga uuuuagccug     4080

aucgacaucg auaaaaaggc uaacuguauu caaaagcacu ucacagaaga ccugguuaaa     4140

gauuuuguga ucguggacga gucugucacc ggccucuucg ugaagaaaga gcgccugaag     4200

uga                                                                   4203


<210>  4452
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4452
augaagacca ucacagagga cuacauucug ggccucgaua ucgggacgaa cagcugcgga       60

ugggccguga uggacaaaaa gaauagacug cuucggcugc gcggcaaaag agcuauuggg      120

ucccaccucu ucgaagaggg acauucugcc gcggaucggc gcagcuuuag aaccacacgg      180

cgcagacuga agcggcgcaa auggagacuu cggcugcucg aagagaucuu cgcugaaccc      240

auggccaagg ucgacccugc cuuuuucguu cgccugcacc aguccugggu gucuccgcug      300

gauaaggacc ggaaaaagua ucaugcuauu guguuuccca ccgccaaaga ggaucacgag      360

uucuaccaug acuauccuac aaucuaccac cuccgggaug cucugaugcg cgaagacaga      420

caauuugauc uucgggagau cuuccuggcc auucaccaua ucgucaagua uagaggcaac      480

uuucuccagg acacccccgu gaauagcuuc guugcgucca aaauugaugu ggccucuuuu      540

cugacaaacg ucaaucaggc uuacgccgac aaguaugggg aagaacagaa cuucaucgag      600

cuuaaugaaa acaaugcugc cgcgauugag gccgugaucc gcggaaagga caaagauaag      660

accacgaaac aacuggacaa ggcuaaaaag auuaccaaac uccugcagag cuccacaaac      720

aaguacgaaa agaauguuac ccggcagauc gccaacgcua ucauuggcua caaaacacag      780

uuugagacca uccugggcaa ggauauugac aaaaaugaua aggccgacug ggaauuuaaa      840

cucagcgaug gggacgccga ugacaagcug gagaaccuuc ugccugaguu cgaugacaau      900

gaccaauaua ucguggcuga aauuaagaaa cucuuuggau cuaucgcgcu gucccagauc      960

gucuacgaga acaugacgcu uagccagucu augaagugua aauauaagga ucacggcgac     1020

gauuaccauc ugcucaaaca guauauugcc acacuguccg accacgggca agugagaaag     1080

cugcuccugg cuuacgaucu uuaugugaac aaucgccacg gacggcugcu cgaagccaag     1140

aaagaguuca agcaggacaa agcccugacc caggaugaau uuuacaagau cgucagagag     1200

aaccuugaca auagccaacu ggcucagaaa auucuccaga agaucgagga agagaaguuc     1260

augcccaaac agcgcguuaa caagaauggc uuuauuccuu accaacugca ucagaucgaa     1320

cuggaucaga ucauugccaa ccagucuaaa uauuacccgu uccucgcgga ggaaaauccc     1380

gugaaggagc accggaaaca agcuccuuau aagcuggacg agcuugugag auuucgcguc     1440

cccuacuaug uggggccucu gaucuccccc gaugacaaac ggaaggauaa gcagcagaac     1500

aaaaaucaug aaauguucaa guuugccugg augaaaagaa aggacaacaa uggagccauu     1560

acaccgugga acuucgcuca gaaaguugac cgcgagacca gcgccaaucg guuuaucaag     1620

agaaugaccg ugaaggauac auaccucauu ggcgaagacg uccugccugc ucggucccug     1680

cuuuaucaaa aauucgaggu gcucaacgag cugaauaaca uccgcguuaa cggcaagaga     1740

cuggaaccca aauuuaagca gaaaguguuc aaugagcugu ucaagcagaa gaacaaaguc     1800

acgaagaaag ccuuuaagga guucauucug cagaauaccg ggaugaaaac agcggagauc     1860

gauggacuug ccgacccuac caaguuuaac ucuagccugu ccagcuacua ccaccucaag     1920

ucuacaaaag uguucgauaa gcaacuggac gaucccgaau aucaggcuga cuuugagaaa     1980

aucauugagu acuccagcau cuucgaagau gccgagauuu aucaggaaaa gcugcauaaa     2040

gagaucaagu ggcuuauucc cgcucaauuu aaagccaucg cgaauuggcg gcuccagggc     2100

ugggggagac ugucucgcaa gcugcucguc gaacugaagg gaaaaggcgg ggagaacauc     2160

auuuaccagc uuuggaccac gcagaagaau uucaugcaaa ucgugaacga gccugaacug     2220

aaaaaggcca uuguuaaaga gaaucaccag gcuacagaag agaagggaau cgaaaagauu     2280

cucgacgaua gcuuuaccuc cccggccaac aaaaaggcua ucagacaggu gaucaaaguc     2340

guggacgaug uugugaaggc cgccggcggc aaaaagcccg cucaguucgc gauugaguuu     2400

accagagagc cuaaguggaa uccccaacug ucucacaucc gcgggacaaa acugcuucag     2460

gucuaugaag acaccgccaa gaaacucgug gaccagagcc ugacagauuc ccugaaggag     2520

aacaugacga auagaaaccu ccugaaagac gaauacuucc uuuauuuuau ucagugcgga     2580

cgggaugcuu acaccggcaa gaagaucgac auugccgaua uccauaaaaa guaccaaauc     2640

gaccacauuc ugccucagag cuucaucaca gaugacucuc ucgauaaucg cgugcugacc     2700

gagauugaga ucaaccagaa aaagcauaaa aaguaugccu cccaccuguu cgcuacaaag     2760

cccgccuuug ggcuuaacca aagcauuucu gaaaugugga aaaccuggca gaaggcggga     2820

cucaucuccc agagaaaacu gaauaaccuc cucuucgacc cggaucuggu uaaggacaaa     2880

cagaagaaag gcuuuauuaa ucggcaacuu gucgagacga gccagaucau caagcuggug     2940

uccauuaucc uccaggacaa guucccugau accgaaauua ucacagugaa agcuggguac     3000

aacaccgccc ugcgcaagga gcugaaacuu uauaagucua gagaagccaa ugacuaccac     3060

caugcuauug augccuaucu gacagcgauc aacgacaauu uucucuacca ggucuauccc     3120

aacuuccggc cuuucuucgu guacggccaa uuuaaaaagu ucagccagga uaccgacaag     3180

cagaaagagg cuaucaagua cuauggacug aaaaauuuuc ucgagccccu gcuugaugac     3240

uccacgggcg augaaguuau ggugaaccac acaaagcaug ucguguucca caaacagcgc     3300

gacauuuuca auagacugau ccaagccuac aacuauaagu acaugcucgu uucucgggag     3360

accacaaccg aaaauagcaa cauguuuaau augaccauuu auccucgcgg ggagagagau     3420

cggaagaaaa caagaaagcu gauccccaaa ggaaaggacc uggauccggc cauuuacggc     3480

ggguauuccg gaaacacgga cgcuuacaug gugaucguca aaaucgaaaa gaccaaggag     3540

acacuuuaca aagugguugg cgugccuaug cgcgcccugg cuagccucaa gaaagcccag     3600

aaggagggga acuaugacca ggaacugaau aaaguccucg agccccugau uauguucaac     3660

aagaauggca aaucuaaggc gggaaucaag ggcuuuaccg uggugaaaga ucauguccac     3720

uucaagcagg uugugauuga cggggauucc aaauuuaugc uuuacagcgc ccaauaucgg     3780

gcuaacgcca agcagcugac acucucucag gaaaccauga aaguggucac ggacaauaca     3840

gaugacaugg aucaaaagaa cgcugacaag gaucagcugc ugcuuaaugc cuacgacgag     3900

auccuggaaa aaguggauca guaucucccc cuguucgaca uuaacggauu uagacagggc     3960

cuccacgaug ggcgcgccaa guucgcugcg cugaaaaccu ccgagaagaa auacacccuu     4020

aagaauaucc ugaacggacu gcaugacaau cucguuacac cuaaccugaa gaauauuggc     4080

aucaaaaccc cguuugggcu uaugcaaagc ucuagcggca ucacacuguc ccccaacgcc     4140

aagcucgugu uccagagccc uacgggacug uuugagaaac gggucaagcu caccaaucug     4200

uga                                                                   4203


<210>  4453
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4453
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc ccuugugaaa       60

gagacggaaa auuccaacga gaagucugaa aucauuaaac ugggcgucag aguuaauccc      120

cucaccgugg augagaagac aaacuucgaa gcugggcggc cucugagcac caaugccgac      180

cgcacagcga aaagauccgc ucggcgcaac cuucagagau acaagcugcg gcgcaagaau      240

cucaucgauc ugcugauuaa acacagacuc aucgacaagg auaccccgcu gacagagauc      300

gggaaaaaca ccacgcauca aacccuugag cugcgggcca aggccgcucg cgaaagaauu      360

gagcucgaag accuggcccg gguguuucuu gcuaucaaua aaaagcgcgg cuauagaucu      420

agccggaagg ucaacaauga ggaggaagga caggugguug auggaauggc cguggcgaaa      480

aagcuguacg acgagaaccu cacacccggc caguaugccu acgaacugcu guccaaaggc      540

aagaaauaug ucccugauuu cuaccgcucu gaccuccagg cugaguuuga uagcauuugg      600

gaauaucaaa agcaguucua cgccgacauc cuggaugacg agcuuuacaa ugcucugaaa      660

gggcagggac agcaaaacuc cagaaagcgg uuucucgcca uuaagggcgu guauaccgcg      720

gagaauaaag ggaagagaga cgaaguuaaa cugcagcacu acaaguggcg cagcgaggcc      780

aucacacaga aacuuucuau ugaagaggug gcuuaugucc ugguggaaau caacaacgau      840

cucaauaagu ccagcggaua ccugggcgcc aucucugacc gguccaagga gcuguauuuc      900

aacaaagaga ccguggggga aaaucucugg aagcagauuc agaaaaaccc ccauacgagc      960

cugaagaauc aagucuuuua cagacaggau uaucuugacg aguucgaaca aaucugggag     1020

acacaggcuc aguuucaccc ucagcugacc cucgcccuga aagaacaaau ucgcgauguu     1080

gugaucuucu accagcggaa acuuaagucu cagaaaggac ugcucuccuu uugccaguuc     1140

gagagcuggg agauugaaag aaaggacgag aacggcaaug ugauccugaa caaaacaacc     1200

caacugccca agcgccagac cgucggccgg agaguggccc cuaaauccuc uccgcucuuu     1260

caggaguuca agaucuggca gaauauuaac aaucuggaaa ucgcuaagau uagcgauggg     1320

aacuccaaaa auaagaaaga gacagaagcg cuuagcgacg augagcgcaa gcuccuguuc     1380

gaggaacuga accugcgggg aaaucuuucu caaaaagaag uucuccagau ccugggccug     1440

aaggacaaag aguacaagac gaacuuuccc gaggggcucg aaggaaacag aaccaaugcc     1500

gcucuguuca acauuuauca gcagaucgcc gagaaugaag gcuacgggga uuggacaaag     1560

aaauccgccc aagagaucaa ggaggaacuu aaagcugugu uuccucagau uggaauccag     1620

gccgacauuc uggacuucaa cgcggagcuc gauggcaagg aauuugagaa ucaagcuagc     1680

uaucagcugu ggcaucugcu uuacucugcc gaagaggacg auaaaaucaa cgaagaggac     1740

cagauuaucu auggcaauag cgccgucucc cucaagaaga aacuguguga gaaguucggg     1800

uuuacccccg aauacgcuaa auggauugcc aacgugucuc ugcaggauga cuauggaaau     1860

cucagcacaa aggcuaugcg caaaaucauc ccuuaccuga uugauggcaa cgacuacucc     1920

gaggccugcg cgcuugccgg guauaaucac agcaacucuc ugaccaagga agagaaugau     1980

aaccgggaga gacucaauaa gcuggaacug cuucccaaaa acucccuccg gaacccgguu     2040

guggagaaga uccugaauca aauggucaac guggucaauc aggugauuga aacguacgga     2100

aaaccugacg agguucgcau cgaacuggcu agagagcuca agaaaagcgc cgaggaacgg     2160

gcugagauga caaagggcau uaacgaagcc accagacgca augaggauau caagaaacug     2220

aucaccaaga acuucgggau ucccaauccu accaaaaacg acgugguccg guauagacuu     2280

ugggaggaac uggcgccccu cgccuacaag gauguguuua caggccgcca gaucaaaaag     2340

gaggaccugu ucucuuccaa aauugacauc gaacacauua uccccaaggc ucugcuuuuu     2400

gaugacagcu ucuccaauaa gacacucgcc uuuagagaga cgaaccugaa aaaggcugau     2460

cggaccgccu auggauucau cgaaucugac uacaaugcca cacuggauga cuauauucag     2520

cgcguugaga cccucuacaa caaugcuaaa ggcacaauca gcaaggggaa aagaaacaag     2580

cugcuuaugg cgcaaaagaa ucugccugau ggauucauug aacgggaccu ccgcaacucc     2640

caguacaucg ccaaaaaggc uaaaucuaug auugagaagg uguuuaacga ugucaaugug     2700

accagcggcu ccaucacgga cagacugcgg gaggauuggg accugaucaa cgugaugaaa     2760

gaacuuaauu ucccgaagua uaaggcccug gggcucaccg agauugagga acgcuacgau     2820

aucggacagg agaaaacaaa gaaaguugaa gucauuaccg acuggacaaa gagaaacgac     2880

caucggcacc augccaugga ugcucugacc guggccuuua cgacaaaaag ccacgugcag     2940

uaucucaaua accugaauac cauccacucu cuuaagggcg acgaggucga ucuggaacuc     3000

uccaagcugu acgggcugaa aaacacaauu accgaggugg uuaauaagaa acgcaaguuc     3060

auucccccua ugcccaacuu uagagaagag gcgaaaaagc auaucgagac cauccuugug     3120

agcaucaaga auaaaaacaa ggucuauaca aagaauauua acaagacgaa aaaggaaucu     3180

ggcuaccaca ccaaagagca acugacaccu cggggacagc uccauaagga aaccguguau     3240

ggcaagucca aaagacccau gaauaagccg acaaaaauca acaagaauuu cagccuggag     3300

caggcucaac ucauuaucaa cguucaggaa aaagagcugg ugcuucgcca ccuggcccag     3360

uuugacaaca auccugccgu cgcuuucgau accaagacgc ucaagaaaau gccccugcug     3420

aagaacgggg agccucuuaa agaagugcug uguuuugagg aaauuuucac aauccggaag     3480

gacaucaccc ccgaucucaa aauugagaag guuguggacg aaaagaucaa agccauucug     3540

gagcagagaa agaaagauuu uggaggcaau gcgaaggagg cuuucucuga ccucgauaaa     3600

aacccuaucu ggcugaauaa ggaaaagggg auuugcauca aacgcgucac caucacagga     3660

gugagcaacg ccgaggcccu ucaucaccaa aaggaccacc ugggccggga aauucuggac     3720

gauaauggga accccauccc ggcugacuuu guguccaccg gcaauaacca ucacgucgcc     3780

auuuacgagg augagaaagg aaagcuccag gaaaaaguug ugagccugua cgaggcugug     3840

gccagagcga aucagggccu uccugucgug gacaaggggc ugaacgaaga ucucggaugg     3900

aaauuccugu ucacaaugaa gcagaaugag auguuuguuu uccccaacga cgauuuugac     3960

gugaaugaua ucgaccuccu ggaugaaaac aacgccaaga uuaucucuca acaucuuuuc     4020

cgcguccaga aacuggcuac gaagaauuau auguuucggc accaccugga gaccaacgug     4080

caggagguua aagaacucaa ugacauugcc uacaagucca ucagaagcac agagccccug     4140

aaaaacauca agaaggugcg cauuaaucau aucggcaaaa uuguccaggu gggggaauau     4200

uga                                                                   4203


<210>  4454
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4454
auggccaaga aagacuacac caucggccug gauauuggga caaacagcgu gggaugggcu       60

aucauugacg auaaucucaa gcugcuuaaa agaaacauga cgaucaaggg caauaccgac      120

aaaaaguccg ucaagcggga ucuguggggg ucucuccugu auagcggaaa cuccgacaaa      180

acaaccucug ccgcggaugc ucgcagcaag agaggccuuc ggcgcagacu gcggcgcaga      240

aaauaccggc ucgaccgccu gaagcagauu uucuccgaga ucaucaauga uaaagccccc      300

aacuuuuucg acaagcugaa ugaaucuuuu cucaacccua aggauaaaaa guaugggaaa      360

uaccaaauuu ucgacacaga gaaggaagag aaagauuauu acagacggua uccgaccauc      420

uaccaucugc gcaaggaccu uauugaaagc uccaaaaagc aggacaucag acugguuuac      480

cucgcccugg cucacauucu uaagagccgg ggaaauuuuc uguucgaggg caacaucgau      540

gaccucaaaa augauuuugc cggcauuuau gaggaagugg uggagcugug caugacaauc      600

aacgcugaag acgucgaucu ggaguucgag gaaguggaca agcagucucu caauuccauc      660

auuaaaaacg aggauaucag cgaaauugag caggggcugg aaaauuuugc cgacgagcac      720

guuaucuuca aggagcaaaa caaaaagaag aacgaucugu uuucuaauug uugcaaaauu      780

aucuguggac auaccgugaa ggcgaacaaa uucgccuccg aacuugacag cgagcucuuu      840

aucucuuuca aguccgauga cuacgucgac gugauugaug uuauccagag cggcaaugaa      900

aacauugcua aucugcuucu ggccugccgc aaggcuuaug acuacaucau guucaacaga      960

cucguggauc ugaauauuga cucccccgcc aaacugucua gcaacauggu cucccucuau     1020

aaucagcacg agaaggaucu gaaagcguac aagaaacuua ucaaggaauu uaacaaauuc     1080

aagcggagca auggguguaa ggaccuggag augaucauuc ucacggccga ugacaucgau     1140

ucuuuucgca aaaaggugga caaaaaggag ggaaaacuga acggcauuaa uaagaaaauc     1200

acccaugaac aggcucuuaa gaagcaacug aaagauauga agaaaauucu cgaggacaag     1260

aacacagaag ccgaggauaa acagaucaau gacauucuga agaugaucac cuccaucgag     1320

gaaagaguga acaagagcug cuuccugaaa aaccuccggu cuacagacaa ugcuagcauu     1380

ccuaaccaga uccagagaca agagauggaa gccauucugg auaagcaggc caaauuuuau     1440

cccuuccuua augagcacaa ggacgaacug cuccagcugc uguccuuucg caucccuuac     1500

uacgucgggc cccuuguuaa caaaaaguau ucucgguucg cuuggcucgu gagaaaggag     1560

ggacaagugc agaaaauuac cccuacgaau uuugauggcg ucguggacaa gcacaaaaca     1620

gcggagaagu ucauggaacg ccugaucggc aaagauguuu accugccgaa cgagcgggug     1680

cuccccaagg ccagccugcu uuaucaggaa uacuguaucu uuaaugagcu gaccaagguc     1740

gcuuauauug acuccacagg gaaaaagaac aauuucagcu cugaagagaa acucaacauc     1800

uuugagaagc uguucaaaac caagagagaa gugaccaaaa cagaucugug caagugucuu     1860

aauaacguuu gcaagcucaa agagaaggug aaagaaacgg agauuaucgg aauuaaggcc     1920

aaauucaaug ccaaguacuc caccuaucau gaccugaaga aaaucaacgg cauggaacag     1980

cugaucgcug augaggaggg gaagccucuc ugugaagaca uuaucagcau ucugacaauc     2040

uuugaggaua aagacauucg ccuuguccgg cugaaggaac uccugugcca aaauaaagau     2100

cugaucaaca aguucucucu uuccgccgag aagcucgcga aagugcugag caccaagcac     2160

uacaaaggau uuggcaacgu cuccgcuaag cugaucaaug ggauuagaga caaaaacugc     2220

aagacaaucc ucgacuaccu gauugaagau gacaaggagg ccuauuacgg acgcaauaac     2280

cccaaucgga accuuaugca gcuggugaau gauucuagac ucgccuucaa aggccagauc     2340

gaccgggagc agaacaccca ucuggaagau cugagccuug acgaguuucu cgaugaccug     2400

uauguuuccc cuucuauucg cagaggcauc cggcugacga uuagacucgu ggaugaacug     2460

gucgagauca ugggguaccu ucccaagaau aucgugauug agaugccgcg cgaagacgga     2520

gagaaaggca agaucgcuga uacacgguau agcaaacugg aaaagaugcu caagaaagac     2580

gccgcucugg aggaccugua cagaguucuu aagaccuaug agaaaaacaa gaaagcccug     2640

gcgaaugaug cccucuaccu guacuuccuc caaaacgggc gcgacaugua uaccggaaag     2700

gaaauuaauc uguccgagcu ucacagcuac gauaucgacc acauuauccc uaaaucuuuu     2760

aaguaugaug acucccugga uaacaaggug cucacagcua aaaagaugaa uauggacaaa     2820

agaaccggcg cccuggauca uaacaucauu gaaaaccagu guggguucug gcggguccug     2880

cuucagcagg acaagaucag ccuggagaaa uacacaaauc ucaugaagac ggaauuuacc     2940

gaggcugaua aggccggcuu cauuaugcgc caacuggugg aaacaagaca gaucaccaaa     3000

uuuguggcgc gguaucucga caacaaguuc aauggacuga uuucugaucc caacgacaaa     3060

guuaauaucc uucugccucg cgccucccuc ugccaccagu uuagagagac auucggcuuc     3120

uacaaggucc gggagcugaa cgacaugcau cacgcucaug augccuaucu gaaugcugug     3180

aucgccaaca cccuuaauaa aaacgccuac cugagcgacc uccugaagua cggggcuuau     3240

ucuaaguaca aaaagaaugg auuuaacaau agcaacggca uuauggauua uuucgggaau     3300

acgcaguuua acugucucuu cguggucgaa cgcacccugg acaaaugcag agugaauauc     3360

guuaagcacc ccgagacagc guccggagaa uuuuacaacg agaccauuca gaaaaacaag     3420

gugaauggcg ggagcucuac acggucccuu aagagcuccg ucaaagugcu gcaaaacacc     3480

gaacaguaug gcggauucac gaauguuaac aaugccuacu uuauccuguu cgauuauaag     3540

gcuaaaucua agcucaaacg caagcugauu ggcgugccca ucgucgacag acaaaaauuu     3600

gagcaggauc cugugacaua ccuugaggcc aagggguucg acgaaccgaa gcugguucag     3660

aaacuccuga aguacacccu ccuggaguau gaagauggaa aacggagaua ccuuacaggc     3720

gugaccggga agcgcuguga gcugguccgg gccaaccagc ugcuccugcc cagaaauaug     3780

auggcccuuc ugcaccaucu ccaagagugg cagaaacacg acuuuggaau uaaggaaaug     3840

accaagguga ucaaaaacac aaauaacauc gaggcuaagu ucgauaaacu guucgaacau     3900

augaugaagu uuauugacaa auauagcgag ccucccaaga ucguguccuc uaagauuagc     3960

gaagaguacc acaaacuccg cgagucccug ugccaggaug acaauaagau caaaauuuau     4020

gcggaaaucg gcaaggcccu ucugucucug cuccaccugg ucgacagcaa auccgcuugu     4080

guuuucaagu uuagcgggcu ugagaucaac cggauuagau accagucuau caaugaaaag     4140

aaagagccug ugauuaucuu ccaaucccug agcggccucc gcgaaucucg guauaaguac     4200

aaccaguga                                                             4209


<210>  4455
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4455
augaagcuga gaaacgccaa agacuacagc aucggccucg auguggggac cggauccaca       60

ggcugggcug ucacgacccu ggacggggau cuuuucacau uuaaucaccg gcccacccug      120

ggaucucgcc ucuucgagag cgccuccaca gcgcaggaaa ccagagaggc ucggggcgcc      180

cgcagacggc ugaagcgcag acgguggcgc auuucucuuc ugcaagaaca guuugccgcu      240

gagauggaaa gcugcgacuc caacuucuuu aucagacuca aucagucucg gcuguggcag      300

gaggaucgca cagagcaagg gaaaggauau cuguucggcg gcaagccuau ucucuguggg      360

gcccagaucu uugcuaacga acaggaguac uaucagcaau ucccgaccau uuuucaucug      420

agaaaauucc uuguugaaug cagcgaacag guggaccugc ggcucaucua ccuggcccuu      480

cacaauaucg ugaaguaucg cggaaacuuu cuguaccagg auaauaagac gcucaacguc      540

agaaauuuca accuggacuc ccagauugcg gauuuucugg accaacucca gcuguggugu      600

gagaauaacg aucuuugcuu ccaugaaauc gacggcacca caacccuggc cagccagcuc      660

aauacauguu uccaccaaca gucuaaccag cagaaaaagg auacccugaa aacguuuuuc      720

gcuacaugca uugagaauga caagaucgcc ggggaacuua ccaaagcucu gcucggauau      780

aagacagacu uuaagauuau cuucaacauu gagggcgaaa aagaugagua caaggccaaa      840

uguaacgagg aagagaagau ccugaaacug caagaaacca ucgacgaugc gaaucaggug      900

cuccugcagu ccauucuuaa ccuguacagc gggaucauug uugagaagau ccucaauaac      960

accgccguga aaaaugaauc ugcucagugc acacuguccg ccuaucauug uuacagcuau     1020

gcucuuuacu cuaagcaacu gcggacgcuc aagaaacugg ucagaaagua uaacccccag     1080

cgcuacaaug agugcuuuaa ccuguccgcc gaggacccua aagccaagaa acagcucugu     1140

accuaugcuc gguacaaugc gcagcacagc acagauuacg acgaguucaa gaaguauauu     1200

aaagaucugu uugagaacac cgacgccgug aaugaugcug ccuaccaaca gaugaagcag     1260

gacuucgaug aagagaaauu ucuuagacgc cagaagacau ccgacaacgg aguuuauccc     1320

uaccaauucc accuggaaga gaugcagcuc aucaucgaga aucaggccca acauuauccu     1380

uuucugacca aaauuaagcc caagcuggaa ucucuuguga cguuccggau cccuuacuau     1440

gucggcccgc ucacacagaa aggcauucag agaaacgggc gcaaguuugc uuggagcaaa     1500

cggaauaagg acgccgagaa ccagagaauc uaccccugga acugggauuc cauuaucgac     1560

aaaagcgcgu cugcugaagc cuucauccug cgcaugaccg gaaagugcac cuaccuguau     1620

ggcgaggaug ugcucccuaa ggagucccug cuuuaccaag aguucugcgu gcugaaugaa     1680

cucaacgggg cccgguggaa uugugacgga gaaagacgca ugccccugag caacaaagau     1740

ugcaaggcua uuuaucggca gcuguuucuu aaaaagcaga aagucucuua cgccgacguu     1800

agcaauuauc ucaaggcuca gggccacauc aacaauuccg uggccggggg acaaggcgag     1860

cauggcuucg aaucuacacu gagcucccac cacuuuuuca gaaaacuguu uaacguggau     1920

gcggccaaug accucaccgc ucagcagauu gagacagccg agcagcugau ccuuuggggg     1980

acgauuuucg aagauaagga gaucuuugaa caaaagauua aagagcgguu cggagacaug     2040

uuuagcaagg augaaaucaa aaccaucgcu cgcagaaagu ucucuggcug ggggaaaugu     2100

uccaagcagc ugcucagcgg acugacaguc gagaccccuc ggggcagaga gucuauuauc     2160

gacgugcugc gcaacgggca ucccgaaaau ggccggacac uuguuuuuau gcaagugauu     2220

accgauaaga aacucgaguu cgacaagaaa aucgaagagu acaacaauga acaccuggcc     2280

aagacgaacg aguccguccu ggacaccgcg auuaugagcc cggccaucaa aagaggagug     2340

aaucaggcuc uccauaucgu uggcgagauu cgcagaauca caaagcacga uccuaccugc     2400

auuuauaucg aaacaacccg ggacgagaag gaugggaaac auaacacgcg cacauccucu     2460

agauacagcu cccugaagaa aaaccuugaa acccugaagu cuauugacaa agccaaugcu     2520

cccgccauca agcacagccu cgagcugcug gagacauaca agaaacagaa caucgaugac     2580

aaacuuuuuc ucuauuucac ccagaaugga aaguguaugu acuccggcaa gagccuggau     2640

auuaacaauc ugucuuccua ucagaucgac cacgugauuc cucaaagcua caucaccgau     2700

aacucuuucg acaaucgggu ccucguguau gccuccgaaa accagcgcaa aagcgauucu     2760

cugcuucuga gcuccgucau ucaggcuaag caggagcccu uuuggacaag ccucgaaaaa     2820

gcggggcuga ucucugagaa gaagcauaau aaccugaaaa gaaagucccu uagcgacgcc     2880

caaaucaaag gauucauuaa ucggcagcug guggaaacgu cccagaucgu uaaggugcuc     2940

cgcgaugagc ugucucggau ucuccccgac accaaaauca uuccugucaa ggcuagccug     3000

ucccacgacg ugcggaucgc cugcaaccuu uacaagucuc gcacagccaa uagcuuucau     3060

cacgcucacg augcccugcu cgcguccacc augggcagau ucguucagac acgguuuccg     3120

gacauguaug agaaccccgu gagauacguc aaaauucugc gcgcuuacau cgcccaaucu     3180

aagcugcagc auagcgccca gacccggaau uucgauuccc gcagcuuuau ggcucacucu     3240

uucuuuugug acuauuccaa cgaaaacggg gauacgaucu ggaauagcgc cucugagauu     3300

gaaagacuuc ggagagcgcu guccuacaaa aaccucuauc ugacacgccg gccuuucauc     3360

gacgcuggcc aguuuuggga ucaaaccauu uacagccccc augacaagaa aaagaccccu     3420

gauucucuca ucccccugaa gaaaaaucuu gacacacaga aguauggagg cuacuccggg     3480

aaagauuaug cccacuucuu cauuuacagc gugcugaaca agcagggaga cguggaguac     3540

agauuugacg gcguuccgcu cuauaucgcc gcuaauauca accgcgauga cagaggggcc     3600

cuggcucaau acgccaaaca gcuggucgaa caugagaaug ugccuaacgc gaagagaaag     3660

accaaagauc uuguguucca gcggauugag cgcgccugcg uccccaagua ucagcugauc     3720

uguauuuccc aaugcagcgg agacucugau agaagcgugu ccgacuacua uuacaucaca     3780

ggcaaaaagg aaguucggaa ugcucuccag cuggccuuuc cuaugcaccu cuauggggcu     3840

auuaacgcca ucgaggauaa aaaggaacug gacgauucug cccuugagga acuggugguc     3900

uacauccugc gcaaugcucc cagaauugcg ccugacgugu uccggagacu caaccugacg     3960

gagaaacuug ccgauugucu ggacaucccc gaucgcauug agcuccagaa uagcaucuac     4020

ggccuguuua accucauuaa ugcugaaucc aacguuguga aucugcagau cuucggaggc     4080

gccaagaccg ccggggucaa acuuauuaac cugaacucuc ugcucaauga cccggcuaca     4140

accgugacau uuaucgacca aagcguuacc ggaauguucg agcggaagga aacgaucggc     4200

auuuccuga                                                             4209


<210>  4456
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4456
augggcaagc aggaguucaa cgacuacuau cugggguuug auaucggaac caauagcgug       60

ggcugggccg ucacagacca acaguacaaa cuccugagau ucaacaagaa agauaugugg      120

gggucccggc uuuuugaaga ggcuaagacg gccaaagaac gcagaguuua ucgcaauucu      180

cggagacggc uggaccgcag aaagcggcgc cucaagcugc uugaggaacu guuccucgau      240

gagauucuga aaguggacag caccuuuuuc aagagacugg aggaauccaa ccucuacaau      300

gaggauaaau cuaucaaguu uaacuauccc cuguucaaug acaaagauua cacagacauu      360

gauuuuuaua aggaauacaa gaccaucuac caccuucgga aagcgcuggu guucgaggac      420

cgcaaguuug auauuagacu ccuguucauc gcccuucauc acauccugaa auaucgggga      480

cauuuuauuu ucgagggcca gagcuucgaa gacaucaagu ccuuugacua cauuuucaca      540

gagauccagg auuggauucu cgaaaacuuu gagaucaaua uugacaucaa ccugaaagaa      600

aagcuggagg agaucauucu cucuaauaac accaaaagca ucaagaagau ugaguucaaa      660

aagaucuuuu ccgauaaucg cauucuggcu auccuuaaca ugggggucgg augcaaagug      720

gaccugaagg auguuuucaa uaacgaggaa cucaaagacc aaaagaucag cuuuacagag      780

auugauuaug aaaacgagaa gagagacgag uucuaccagc ugcuuggcga aggcaucuuu      840

auuguggauc uggccaaaaa gaucuaugac uucauggucc ucaaaaagau ucugaaaaau      900

ucugauuacu uuuccgagag caagguggcu cuguaugagg aacacaagga agaccucaac      960

cggcugaaau acaucuucaa gaaauaccuu aagucuuccg acuauuacaa auuuuucaga     1020

agcaauucug agaagaccaa cuauccuucc uacaucgggc ugaauaagau uaaaacgaag     1080

cgccucaucg agaacaauaa auguagcauu uccgauauca acaaggaacu gaaaaauaag     1140

uaucuuaaag acauucaggu ugaggccgaa gaugaggagc uguucaaccg gauccuccau     1200

aagauuaaug aaaacaaguu ucuggagaaa cugaagucua gcgaaaauuc caccgugccg     1260

uaccaggucc acgagaucga acucaaaaag auccuggaca accaaagaaa auaucacagc     1320

uuccuuaaug agaagucuga uggaaugaca cugugcgaga agauuaucaa acucuuugag     1380

uuccgcauuc ccuacuacgu gggcccucug aacaccaacc ccaauaugaa caagaaaaau     1440

aagaacacau ggguggucaa aaaggaagaa aauuccaaga gcucuaucac cccuuggaac     1500

uuugagaaaa agguugacga acagaaaagc gcugaggagu ucauuaagcg gaugacgaau     1560

agauguacau aucugcgcgg ggaagaugug auccccaaaa acucccuucu cuacgaggaa     1620

uuugugcugc ugaaugagcu caacaagguc accguggaca augaacugcu ucauccuaca     1680

cugaagaacg agaucauuga ugaacucuuc aaaaccaccg uuaaggugac aaagaagaaa     1740

aucacggaau uucugaagaa aaagcaggcc auuucugacg ucgagagcau caccggaauu     1800

gauguggagu ucaauggcaa gcugcagggg uauaacacau uucggaaaau ccuuggagaa     1860

aagaucgaga aucucaacau uaaaaaguuc guugaaagau gcaucgagcu gaaaugucug     1920

uacggcuccg acaagaagcu cuuuaaaaac gcggugggca agcuguauaa agaauacaau     1980

gucaccgaug aggaguucac aaagauuaac aaacuuaauu ucaacaagug ggggcgccug     2040

agcaaggaau uucucaccgg aauccgguuc gagaaaacgg acggcacaga aagauuugag     2100

ucugugauug ccgcucugcg cuccaccaau uauaaccuga uggaacuucu cagcgacaag     2160

uucaaucugc aaucuaaaau cgaugagauc aacggggaag acgauuuuuc caauauugac     2220

aucaaggagu acgucaacga gcuguacgug agcccguccg uuaaacgguc uauuauccag     2280

accuauaaga uugccaugga aaucaagaaa auuacaggaa agaaucccaa aaagguguuc     2340

aucgagaugg cuaaaggcgg gggagaaaag ggcaagagaa ccgagcggcg caaagauaag     2400

aucaaagaac ucuacaagaa caugaaagac agccuguccg auuauugcaa uaaccuucag     2460

gaccugucua gccaagucga gaaguacgau gacaauagac uccggcagaa aaagcuguuu     2520

cuguauuuca ugcagauggg caaguccaug uacacaggga acgauauuga ccuuagcgag     2580

cucuuuaaca auaacaucua ugauauugac cacaucuacc cucagucuaa agugaaggac     2640

gauuccuucg acaauauugu ucugguggaa aaacaagaga accauagcaa gucuaaugaa     2700

cugcucaaac ccgagaucca guccaagaug aacaauuacu ggaucuuucu gagagagaac     2760

aaguucauua gcaaagaaaa guauaaucgc cuuacgcgga aaaagaacuu caccgaugag     2820

gaacugggag acuuugucgc cagacagcuc gugacaaccc gccagucuac aaaagaggug     2880

gcgaagcugu ucgaaaagau cuuugauggc accgagguug ucuacagcaa agccucccug     2940

gugagcgacu ucagagagcg guuugauauu aucaaggugc gcgaagucaa ugacgcucac     3000

cacgcccaag augcuuaucu uaacauugug guugggaaug uguucaacac gaaauuuacc     3060

aagaauuacu ggaaauucau caaggagaac aauagaaaga acgaagagaa cccuaauaaa     3120

aaggaaggau auaacuuuaa aaauaucuac gagucugacg ucuuccggaa cgaggaugug     3180

gccugggugu uuuccaagga aaauaaagag auuaacacaa ucaagaccgu uaauaaaaug     3240

gucuaucgca agacagugaa cauuaccgaa augcugguug agggcaaggg gcaacucuuc     3300

aaucugacga ucaaagacag aacacagcuc aagaaaaagg ugggcaacau ugucgccaau     3360

auccccaaaa gccugaagaa ccggaauacc aaacuuaaau ccgacgaacu ggcuaagaaa     3420

uacggauacu uugauucucu caacaauagc uauuucgugc uguucaagua cguggagaaa     3480

gagaaggaaa agauuggcuu ugacgggauc acaaucgucg auaaagagaa gcugaaaaag     3540

aacuccgaca uugaacgcuu ccuugagaac aaaggaaaua agaacgcgaa gcugaucaga     3600

aaaauuuuua agaaucagaa aguucucauc gauggcuauc cguaccugcu caagucuugu     3660

aacaaagaca aggccauucg guucgauaau uuuaaaagcc uguaucuuga cgagaacgau     3720

cugaaguuca cccgcgacgc uaucaaguuu gaaaagaaua acgccgauau cgaccucaag     3780

aaacagaagu acauuaucuc cuuccugaaa aagaagaaac agaaugagau uaacgaaacc     3840

uaugaggaau gcaucgagag acugaauaac gaguuugaua cacuuuuccu gaaucucugu     3900

gacauucaga agucuccuau cuacaaaaac uacaagcugc ccgacuuuau cagcaaaaag     3960

aauguggugg aagucaagga uaaauucgag guguuuaaga accucgaacu guauaacaaa     4020

uccaaacuua uuaaaaauuu ccugaacuac uucaaguaug acaauaacaa gcuggaucuc     4080

aucgccauug ggaucgacag caaagcuaau ugcauucaga agcauuuuac ggaggaucug     4140

aucaaagacu ucguuauugu ggaugaaucu gucaccggac uuuuugccaa gaaagagcgg     4200

cuggaguga                                                             4209


<210>  4457
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4457
augaccuaca gaaacuauca caucggccug gacauuggga caagcuccgu gggauucgcc       60

guccgguacg auaauggcga gcucguucau aagaaagggu cuaaccuggu gggagcucgc      120

cuuuuuaagg aaggcgagac ggccgaagag agacggcuga aucgcggggc gagacggcgc      180

uauaacagac ggaaauggcg ccucaaucug cuuaaccaga ucuucaagcc ccugauugaa      240

gugaaugacc cuaccuuuuu caaaagacuc gagcaaagca accuggucaa uuacgauaag      300

uccgagucuu uuagccugcu caacaaucag uccgacgaua agcugcacca gcaguucccg      360

acaaucuauc aucuucggga cuaccugaug aaaucuaccg aaaaggcuga uauucgccuc      420

aucuaucugg ccgugcacca uaucguuaaa uacagaggaa acuuucuuga cgccagcccc      480

guggauuccu ucgaggcuag caaucugaag gucgacgaaa uucucacaca aaucaaccug      540

cugcucgaua aucugaccga cgaucuucgg cugaacacac aguacgugac cgacauugag      600

cagacgcucc ugucuaacac cgaguccaau aaagacaagg aaaagguuau ccagaaacuu      660

cugcuccaca aggaggccau uaaaaucgau aaggaccgca cagcucaaau ugccaaacug      720

gugcugggcu auaaggcgga uaucgccaaa cucuuuggca ucaccgaaaa gcugcagaca      780

ccuaaacuug agcugagcgc ugccaacucu gacgaugcuc ucauggaacu guccgccgag      840

cuuaaugagg accagauugg gauccugcuc cagcugaaga cccuguacag cagaguccgg      900

cucaaccaaa uuauccccuc uggaacgucc cugagcaagu ccaugauuga aaaauaugag      960

cuucaccaua cacagcugaa gucuuucaaa gaaaauaucc uccugaagcu gagacgcaac     1020

accgcggugc ggagaaaucu ugagcucgcc uacgcucugu augugggcaa cgcccagaca     1080

aagcaagaag ucuuugagcc uaaaaaggcu cgcuucacca gcgaugacuu ugccucccug     1140

aaagccaaug cuaagagcgc gucuuccguu aaagaggccc ccaagaacac caagacaaaa     1200

cucacgaccg augacuucua caauauccug acagaacuuu ucgcuaccuu ugagagcgaa     1260

cugucugccg augaggacau gauuaaggaa guggagcaga ucaaaaaggc cauuacaggg     1320

gacaacuauc ucccuaaaca gcggaccgau cagaauggaa acaucccgca ccaacugcau     1380

cagauugagc ugagacagau ccuucagaau caacuccccc acuacccuga aauggacaag     1440

aucaacgagc agaagcccau ugaaaaacug gugacguucc gcgucccuua cuauaucggc     1500

ccccugauua caccggagga ucagcagaag uccuccgcug ccaacuuugc guggaugaaa     1560

cggaaggagc aaggggcuau caccccuugg aauuucgacg auaaagugga cagaaugcag     1620

ucugccacca acuuuauuaa gcgcaugaca guuaaagaua ccuaccucau caaugaagac     1680

gugcugcccg aucggagccu ucuguaucag agauucaagg uccucaacga gcugaaucug     1740

gugaagguua acaaucagcu ucucuccauu ggagaaaaac aagccaucua ccacgaccug     1800

uuugagaagc agaaaacagu gagcacgaag aaacugcagu cuuaucucca auccgaacag     1860

ggcuacggcg auaacaucga gauuagcggg cugucuaauc cugacaaguu caacaaugga     1920

cuuaccacau auaucgauuu uaagaacauu uucggcgacc uggucgagga caccacaaaa     1980

cagaaggaau uugagcagau cauugaaugg uccaccgugu ucgaggaucg cgcuaucuuu     2040

aaagccaagc uccaagaagu caauuggcug acggaggagc agaucaaagc ucugguggcc     2100

aagcgguaca gcgggugggg aagacuuucc uaccggcucc uggcggaacu gguuaacgac     2160

aauggcgagc gcauuaucga uauucucugg aacaacaagg ggucuaaucu gaugagcauc     2220

gugucccagg acgaguucca gcaacagauu gccagaauca acucucauga aguccaggaa     2280

cuuggaaugg agagcgugcu ggcuaaugcc uauaccagcc cccagaacaa aaaggcuauc     2340

cggcaaguua ugaaaguggu cgaugacauu cagcucgcca ugaauggcca ggcgcccaca     2400

agcaucucua uugaguucac cagaaagccu gaagagaacu ccgaaaucag caaaucucgc     2460

uacgagacaa uuaccuccau cuaucaaagc cugucuaagg auaucgagaa ggaagugaaa     2520

gacaaucuga acaagaauaa aagcucccuu aagaaaaacc ugagcgauaa guacuaucuc     2580

uacuuuacgc agcugggccg ggacauuuau acaaaucagc cgauccccuu caacgagauu     2640

ccuuacuacg auaucgacca cauugugccc agaucuguug aaaugaauga uucccucaac     2700

aaucgcgucc ugguggccaa aagcaucaac cagauuaaga agaacaaugc uccuaucgac     2760

uccucucuua aaaucaccag cgagacaaga caacugugga agaaacuccu ggacuccggg     2820

cugauuacca agcagaaaua uuacaaacuu ucuagcucca ucaagaacac cgugucuccc     2880

uaugucaagc agggauuuau uaaucggcag cugguggaaa caagccaagu uaucaagcuc     2940

guggccaaca uucugggcac gaaauaccag aaugaugaca ccgagaucau cgaggucaag     3000

gcucaucuca acuccgaaau ucgcaaaaca uucgaguuuu auaagcugag agaacuuaau     3060

gauuaccacc auggguucga cgccuaucug accacacucg ugggaggcua ccuguacaac     3120

cgguauccga agcugcgcag cuacuuuguu uauggggauc acaaaagacu ugacaaggcc     3180

cagcugucug agcucaaaaa uuucaacuuu cugcacggcc ucaccaaucc uaaguccgga     3240

agcgauggcc ugaucacggc uucugggucc cagcaagugg uccuugaccg gaacgaaaca     3300

auuaaacgcc ugaauaagau cuacagauau aaguacaugc ugauuagccg ggagguguuc     3360

accaaacagg gacaguuuua uaacgagacc aucuaccccg cgaagccuaa uacagccaaa     3420

augauccccc ucaaggauaa caaaccuucu gaaguuuacg gcggguauuc cagcuccaau     3480

gugagcaaga uugcucugau caaggagaaa aacaccgaca acuaccaguu cauuggaauc     3540

cccauucgcu cuaucgccaa acuuaagcaa gccaaggcua auagcaaaac agucuaucag     3600

acggaacuga acagagugau uaaguccgug cucgccucuc agaaaaccaa gaagcaaaaa     3660

gaguuugaag ucauccugga uaauguuaag uacaaccagg ugguggucga ccagggcgag     3720

aaauauuacc ucgggagccc guauuacaag aaaaaugcga agcagcuggu gaucuccccu     3780

gagucuguua agauucuugc ugacaaagcc acagccgauc ugcccgaaca agaggcucau     3840

aacaagcuga ugaccgugua ccaggaaauc gucgacgugc ucaauaaaca cuucgcccug     3900

uaugauauua acaauguucg gaagaaacuu aacgacggcc ugaucaaguu cagccagcuc     3960

cccaauuacg augagacauc caagaacaga uuugacauuu cuaaaagcau caagaccaaa     4020

uaugcgguga ucaugcgcau ucuguccgga aucaagaauc agacgagcga ggucccugaa     4080

cucggccuga aaucuccgcu uaacaagaug caaaccaaag ggggaauuaa gaucuccccc     4140

gaggcuaaga uuuacuauag cucuccuaca ggcaucuucu cccauuuuaa acaggugaag     4200

aaucuguga                                                             4209


<210>  4458
<211>  4209
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4458
auggagagaa uccugggccu cgaccugggg gugagcucca uuggaugggc ccuugucgaa       60

gaggauguua agaaccccga aaauaacaaa aucauuaagc ugggcgugcg ggugaauccu      120

cucaccaucg acgagaaaac aaacuucgaa aaggggaaau cuauuacgac caaugcugga      180

cgcacaagcg ccagauccgc gcggcgcaac cugcagagau uuaaacuucg gcgcaagaaa      240

cugcucgaga uccugaucca cuacaagauu aucaaaaaug augcuguccu ggccgagacc      300

ggcaagaaca caaccuucca aacacaguau cucagagcca aagcugcccg ggaaaagauu      360

gagcuggacg aacuugcucg cgugcugcuc cugaucaaua agaaaagagg guacaagucu      420

agccggaaag uucagaacga ugacgaugga aaggccauug acggccuuuc cguggcgaaa      480

gagcuguaug agaagaauua caccccgggc caguaugccu acgaucuccu gaaacgcggg      540

aagaagcaaa uccccgacuu uuauagaucu gaucugcaga acgaacucga ccggauuugg      600

gaguuccaga aacaguacca uccugauauc cugacggacg aauuuaagaa agagcuugaa      660

ggaaagggcc aacgcgcuac cgccgcuauc uuccugaaua gauacaaaau uuauacagcc      720

gacaacaagg ggacccggga ggagaagaaa auccaggcgu accaguggag agucgaagcc      780

cucagccagc cccuggaugu gaaggagguu gcuuauguga uugccgaaau caauaacaac      840

cuuaauaacu ccagcggaua ccugggcgcu auuucugacc gcuccaaaaa gcucuauuuu      900

aaugggcaaa caaucggaga guaccuguuc gaggagcuga gcgccaaccc ucacacccgg      960

cucaaaaauc aggucuuuua uagacaggau uaccaagacg aguucgaaaa gaucuggaag     1020

auucagacga aauuucaucc cgagcugaca aaggaacuua aagaacaggu gcgcgauauc     1080

gugauuuucu accagcggaa gcugaaaucu caaaagggcc ucaucuccuu uugcgaguuc     1140

gaaagcagag agauugaaau cgaggaaaac ggcaagcgca aacggaagaa agucgggcug     1200

aagguugccc cuaaaucuuc cccgcuuuuc caggaguuua agaucuggca agugcugaau     1260

aacauugaga ucaaaaauga agacgagaga cgcuaucucg aacccgagga aaaggagcug     1320

cuguucaacg agcucaauug uaagggaaac cugaccagcc agaaaugccu ugaacugcuc     1380

ggcuuuaagu ccaaagaugc uaagcugaau uucaaagugg ucgaggggaa ccggacaaau     1440

gaaaagcugu acgacgcguu ucuuaagauu cucgagcugc agggauauaa cguguucgaa     1500

cugcucaaaa ucaaggagga uaaagacgag gccaagcugu cugaacuuaa agcuagcgcc     1560

gaugagauua agggcaugau caaggaaauu uuuaacgcca aucugaucaa caccuccauc     1620

cucgacuuca augcugagcu ggaagggaaa gacuuugaga accaggccag cuaccaguuc     1680

uggcaccuga uguauucuua cgagggagau aauuccccua gcggcaacga aaaacuuuau     1740

gagcuccugg aaaagaaguu uggcuucaaa aaggagcauu cuaagauucu gagcaccauc     1800

guguuuuccc aggaguacgg gucucucagc acaaaagcga ugagacgcau uuaccccuau     1860

aucaaggaca auaaauacuc cgaggcuugu cugcuugccg gauauaacca cagcaagcac     1920

ucucugacga aagaagaacu cgagaaucgg aagcugaaag aacaacugga gguucuuccu     1980

aagaacuccc ucagaaaucc cgucguggag aagauucuga accagaugau caaucugguu     2040

aacgccacca ucagcgaaua cggcaaacuc gauggggugc ggauugagcu ggcucgcgaa     2100

cuuaagaaau cugcccagga gagagaagac auggcuaagu ccaucaauga ggccacacug     2160

cuccaucaga aauaugcgac cauucugaag caagaguucg gagucgugaa cccgagccgg     2220

aacgauauca uuagauacaa gcuguauaug gaacuuacag ucaauggcua caaagaccuc     2280

uacaccaaca cgaagaucga gaaagaaaau cuguucacag auaaguauga caucgaucac     2340

auuaucccuc agucccgcuu uuucgacgau ucuuuuagca acaaagugcu gguuccccgg     2400

caguccaauc ucgacaaggg gaaccugacc gccuacgauu ucaugucuaa uaagggcagc     2460

gagagagagg aacauuuucu uaacgugauu aaagagcugc ucgacaaggg auccaucagc     2520

aaagcuaagu augaaaaacu gaagaagaaa ggccuggaga uuggggacgg auucaucgaa     2580

cgcgaucuua gagacaccca auacaucgcc aagaaagcua aggagauucu guuugagauc     2640

acaaauucug ucgugaccac auccggccgg auuacggaua aacuccgcaa ggacugggau     2700

cugguuaaca ucaugaagga acucaaucug gacaacuaua gacggcuugg gcugaccgag     2760

auugugcgca auagcaaagg agaagagaag gaacucaucc cugauuggac aaaaagaaac     2820

gaccaccggc accaugccau ggaugcgcug accgucgccu ucacaacccg caaucacauu     2880

caguaccuga acuaucuuaa cgcuagaaag gacgagaaac auaaggagca ccagaauauc     2940

uacgccaucg aaaacauuau cacggaggug auugaaaaga agaauggcuc uaagacccgg     3000

cgcuuuaaag cucccaugaa cuccuucaga caugaggcca agcagcaccu gaaagaaauc     3060

cucgugagcc acaagaccaa aaauaagguu gucacaucua acauuaauaa gaccaaaaag     3120

aaagggggca uccauaagaa aacagagcug acgccccggg gacaacucca caaggagaca     3180

guguacggca gcaagaaauu ucugcagucc agagaagaga aggugagcgc caaauucgau     3240

uaugaaacca uccuuauggu cgugaacccu auucaucgca augcucugcu caaccggcug     3300

aaggaguacg ggaaugaccc gaaaaaggcg uuuucuggaa agaacgccau caauaaaaac     3360

cccguuuauc uggaugacuc caagacagac auucuuagcg aaaaagugac ccuguccugg     3420

uacgagaccg gcuauacaau cagaaagucu gucacgccug auaauuucaa agacuacaag     3480

aaccucgaga aaauuaccga ucuggggauc aagaagaucc ucaaagaacg ccuggaccag     3540

uucaagggaa augcuaaaga ggccuuuagc aaccuugaua aggaccccau uuggcugaac     3600

gaagagaaag gcaucgccau uaagacagug accaucacag ggguuuccaa ugcugagucu     3660

cugcacuuca agaaagauca ccucggcaag gaaauucugg acgagaacgg aaauaaaauc     3720

ccuggcgauu uugugagcac cgggaacaau caucaccuug ccauuuaucu ggacgaagag     3780

gaaaagcucg augacaaaau ggucacguuc uacgaggcgg ugcugcgggu uaaccaggga     3840

cuccccguga ucgacaagga uuacaauaag gagaaaggcu auaaguuucu gaugacacuu     3900

aaacaaaacg aaauguucgu cuuuccgaau gaggacuucg auccuaacga aaucgaccug     3960

cuggaugaga gaaaucucga acgcauuucc aagcaucugu uucgggugca gaaaaucucu     4020

aagguggggu acggaaacag cuucgucaga gacuuuguuu uccgccacca ucuugagacc     4080

accguggagg aacggaagga gcugagaaau auuacauaua uccagcucaa aucccuggaa     4140

ggccuccgga acauuaucaa ggugcgccug aaucaccuug ggaaaaucgu ccagauuggc     4200

gaguacuga                                                             4209


<210>  4459
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4459
augaccaaga acauccuggg ccucgaccug ggggugagcu ccauuggaug ggcccuuguc       60

caggaggauu cuaaaaaucc cgaaaacaau agaaucguua agcugggcgu gcgggugaac      120

ccucucacag ucgacgagca aauuaauuuc gaaaaaggga agccgaucac gaccaacgcu      180

agccgcacac uggccagauc cgcgcggcgc aaucuucaga gauuuaaauu ccggcgcgcu      240

aaccugauug augugcucaa gaagugcaau auccugaaac agggagaccu gcucaccgag      300

aucggcaaga acucuacauu ucagacccaa gaacugagag ccaaaagcgc caaggagaaa      360

guugagcuuu ccgauuucgu gcggguccug cuccugauga auaagaagcg cggguacaga      420

ucuagccgga aagcuaagaa cgaagacgag ggacagauua ucgauggcau guccguggcc      480

aaaaagcugu augaagagaa ucuuacaccc ggcgaguaca gcuaucagcu ccugauugaa      540

aacaaaaagc agcugccuga cuuuuaccgc ucugaucucc aauccgaguu cgacagaguu      600

uggaaauuuc agaagcaguu caaucccgaa auccugaccg augaccuuua uaaggagcug      660

caggggaaaa acaagaacgc uacgugggcc gugcucaaag aaccuuuuag ccuggucgga      720

auuaagcaaa ucggcaccau ucaggagaaa aaggcggaga aguacuucug gcggucugaa      780

gccgugaaac gccagcuuga uuuugagucc cuggcugugg ucuuccaaga aaucaauagc      840

aaccucaaua acucuuccgg guaucuggga gccaucagcg acagauccaa ggagcuguac      900

uuuaauaacc agacaguugg cgaauaccuc uuccagcagc ugaaagaaaa uucucacacc      960

aaacuuaaaa accaaguguu cuaucggcag gacuaccugg augaguuuga aaagauuugg     1020

gagacacaga gcaaguauua caaagaacuc accaaggagc ugaaagaaga gaucagagac     1080

auuaucauuu ucuaucagcg caaacuuaaa ucccaaaagg ggcugaucag caucugugag     1140

uuugaaaauc gggagauuga aaucacggag aacggaaaga uuaaaaagaa aacagugggc     1200

cucaaggucg cucccaaauc uuccccucug uuccaggagu uuaagaucug gcaagugcug     1260

aauaaccucg uuuuccagag cauugaaucu aaagagcugu uuccgcuuga ucuggacauc     1320

aaggaaagca ucuucaauga ggugaacauu aagggcaauc ucuccgccaa aaacguccug     1380

gaucugaugg gguacucugg aaaggaaugg aaaaccaacu uuaagaauau cgagggcaac     1440

agaacaaaug agaaccuuua uaccgcguuc cucaaaauua ucgccagcga agggauugag     1500

uuucccaagg aguucaagcu guccaucgac gaugacauua aagugagcaa gaucgauauc     1560

ucuuccgaga aaauuaagga auuuaucaaa gagaauuucc ugaagcucgg aauuaacacc     1620

agcauccugg acuucaaucc ugagcuugau ggcaaggacu uugaaaaaca ggagucuuac     1680

cagcuguggc aucuccugua cuccuaugaa ggggacgaua gcgcuuccgg aaacguuaag     1740

cuguacgagc uucucgaaaa gaaguucggc uuuaaaaagg agcacucuaa gauucuggcc     1800

gacgugggcu ucagccccga uuaugggucc cugucuagca aagcuaugcg caagaucuac     1860

ccuuauauca aagagaauaa guacuccgac gccugccauc ucgccggaua uaaccacagc     1920

aaaaauucuu uuacaaagga ucagcuggcu aaccggauuc uuaaagaaca gcuggagauc     1980

cuccccaaga augcgcugag aaacccgguc guggaaaaga uucugaauca aaugaucaac     2040

cuugucaacg aggugauuaa agccuacggc aagccugacg agauccgcau cgaacucgcu     2100

cgggagcuga aaaagaaugc cgaagagaga gccaacauga cguccgauau uagcaaagcu     2160

acccugcucc aucagaagua cgccgaaguu cugcagaagg aguucgggau caaagcgccc     2220

ucucgcaaug acauuauccg guauaaacuu uaccuggagc ucgaaaagaa ccuguuuaag     2280

gaucuguaua cagacgugca aauuaagaga gaggaacuuu ucaccgauaa guacgacauc     2340

gaucacauca uuccucaguc ccgcuuuuuc gacgacagcu uuucuaauaa gguccucgug     2400

ccccggggag cuaaccugaa aaagggcaau uucacagccc uggauuauau ggagaucgaa     2460

gggaaagaga agcucgagaa auuucugaac auuaucaagg aacuuuucga caagggcgcc     2520

auuagcaaag cuaaguucga gaaacugcag aagaaaggau ccgaaaucgg cgagggguuu     2580

auugaaagag aucuccggga cacccaguac aucgccaaga aggcuaaaga gauccuguuc     2640

ggaauuacga auagcguuau cucuacaucc ggccgcauua ccgauaagcu gagagaggac     2700

uggaaccuug ugaauaccau gaaagaacug aaccucgaga aguaucggaa acuggggcuc     2760

acagaacuga ucgucaauag caagggagag gagaaagaaa gaauuaucga uuggaccaag     2820

cgcaacgacc accggcauca cgccauggau gcgcuuacag uggccuuuac gacccauaau     2880

cacauccaau accugaacua ccucaacgcu agaaaggacg agaaacacaa ggaacaucag     2940

aauauuuucg ccaucgagaa cacaauuacc gaaaucgugg agaaaaagaa uggcuccaaa     3000

gagcgcagau uuaagcugcc cauuaagaac uuccggacag aagcuaaaaa gcagcuggag     3060

gaaguucuua ucucucacaa agccaagaau aaagucguga ccaagaacau caauaagauu     3120

aaaaagaaag ggagcaucau ugcgaagacg gagcugaccc cucgcggcca gcuccauaaa     3180

gaaacaaucu auggauccuc uaaguuucug aagaccaaag aggagaagau uagcggcaaa     3240

uucgaucucg aaaugaucca aaaggugcag aacgagaaau acagaaaugc ccugcuuaag     3300

cggcugcagg aauuuggggg agacuccaaa aaggcuuuca caggcaagaa cgucauuucu     3360

aaaaauccga ucuuucucaa cgccgagaag accgaacagc ugcccgagac ggugacacug     3420

gcuugguaug agaccgggua cacaauccgc aaaguuguga auccugauaa cuucaaggac     3480

uucaaaaaua uugaaaaggu caucgacaag ggagugagag auauucuuac cgagcggcug     3540

aaagaauuua acggcaauag caaggaggcc uucuccaacc ucgagaaaaa ccccaucugg     3600

cugaauaaga gcaaagggau uucuaucaag accguuacaa ucacgggcau uaacaaugcc     3660

gaagcucucc acuauaagaa agaccaucug ggaaaggaga uccuugauga aaacggccaa     3720

cgcauugcgg uggacuuugu cuccaccggg aauaaccacc acguggccau cuacgaggau     3780

gacaauggaa accugcagga aaaaguugug agcuucuaug aggcugucga gagagugaau     3840

cagaaccugc cuauuaucaa uaaggaauac aacuuugagc ucggcuggaa auuccuguuu     3900

acaaugaagc aaaaugaaau guuccuuuuu cccucugaug acuucgaucc gaaggaggug     3960

gaccuguuug augacaaaua ccucucccug aucagcaaga accucuuccg gguccagucu     4020

auuuccaucg uucaguaugg gaauagcgug auucgcgacu uuaaauucag acaucaccug     4080

gaaaccuccg ugagcgauga gaagaaacuu cagggaguca cauaccaaca gaucaagucu     4140

cugcagccuc ugaaagacau uaucaagauu cggcucaaca accugggcaa gaucgugcag     4200

aucggggagu auuga                                                      4215


<210>  4460
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4460
augaagggca acaucgagaa aaagaaacag gaguucaaug acuacuaucu gggguuugau       60

auuggaacca acagcguggg cugggccguc uccgaccucu cuuacaaucu gcuuagauuc      120

aacaagaaag auaugugggg agcucggcug uuugacgaag ccaagacagc gaaggaucgc      180

agaguuaaac ggaauagccg cagacggcuc gaacgcagau ccaagcggcu gaaauaucuu      240

gaggaaaucu ucgagacgga gauuugcaag aucgacaaaa acuuuuuccg cagacuggaa      300

gagucuaccc uccacaauga agauaagaca uuugacuucc gguuucccau uuucaacgau      360

gagaauuaca ccgacaagga guauuacaaa gauuuuccua caguguauca ucugcgcaac      420

gaacugauca agaaugagaa caaaaaguuc gacaucagac ucauuuaccu ggcuauccac      480

aauauucuua aauaccgggg gcauuuuauc uucgaaggcc aagauuucga cagcgugacc      540

gacuuuaagg aggucuucuc cgaacugucu gccuaucuca gcgagaaccu ggaggaaaau      600

cuugauauua accuguguga caacaucaaa gauauuaucu gcuccaagga gaauaagaca      660

agcaaaggga agucuuuugu gucccucauc gacagcaaag uuauucaguc ugugcugcgc      720

uguugcaucg gauacagagu caaguucaaa gauauuuuua ucaacuauga agagaaggaa      780

aagaaugagg gcauuaaccu guccgagauc gaauacgacg agaaacggga ugaaaucgcc      840

cucagccugg gcgagaauau ucuucugauc gacauuugua agaaagugua ugauuucaug      900

auccucaaca auauucugaa ggggaacaaa uacaucucug aauccaaggu uaaucuuuau      960

gacgagcaca agugcgaucu guguaaccuc aaauacaucu uuaagaaaua ccugccgaag     1020

aaagacuauu ucgaguuuuu cagaaaugaa aacaaggaga agaauuacgu gagcuauauu     1080

uccucuagcc ugaacaaugg aaagaagaaa aauaccugca cgcaggaaga ccucaacaag     1140

gagaucaaag aguacauugc uaagaucaaa gaauaugagg aaaacgaugc cguccuggag     1200

aagauuaacc uuaagcugaa ugaaaacaaa cuccugccca agcugaaaac caaugacaac     1260

ggcgugaucc cuuaccagau ucauaagauc gagcuugaga aaauccucca aaaucagucc     1320

aaguauuacg auuuucugcu ggaaaagauu gacgggauca caaacauuga gcgcaucgaa     1380

aaacucuuca ccuuucggau ucccuacuau aucggaacac ugaagggcaa auucagcugg     1440

gcuaagcuga aagagaauaa gaagaucacc ccuuggaacu ucgaaaagau uguggauaug     1500

gaggagucug ccaaggcguu uaucgaaaga auguccaauc gcuguagcua ccuuaaaacg     1560

gagaacgucc uccccaaaaa uucucugcug uauagcgaau acgagcuucu caacgaacug     1620

aauaaaguua agauuaagaa cgaguucauc aacguggugc ugaaaaauga gauuaucgac     1680

gaacucuuua agacaaccuc caaagucaca aagaaaaagu ucguggauua ucugaaaacc     1740

aagcagaucu uuguugagaa ggaagacauu ucugggcuug auaacgaguu caaaagcaag     1800

cuggacuccu acaucaaauu uaagaaagug auuggagaua agaucgacac ccuccggggc     1860

uauaauuucg ucgagaagug cauugaauuu aaauguuucu acggcgauga caagaaacug     1920

uuuaagaacg ccaucaauaa cacagaguuc aaagacuacg ugagcgauga cgaacugaag     1980

aagaucaaua aaaugaaguu uaacaaaugg gggagacuuu cuaagcgccu ccugacggag     2040

auuugcuucg aaaauguuau cgaguccgag agcuucgaaa acauuaucgu ggcucugcgg     2100

ucuaccaauu auaacuuuau ggaguucaca uccaaaaagu uuaaucucag cgaaaagguc     2160

gccgaggaaa accaggagaa uacccuggag aaauucaaca uuaacgaaua cguggaugag     2220

cuuuaugucu ccccuucugu gaagagaagc aucauucaaa cauacaaaau cgcuauggaa     2280

aucaagaaaa uuaccggaaa gaauccgaag aaaaucuuua uugagauggc ccgcggcggg     2340

ggagaaaagg gcaaaaagac ggagaaacgg aaggagagaa ucaaaacacu guaugcgaac     2400

augaaggacu ccaaugaaga guuccucaac aaucuggaua accugaagcu cucuguugac     2460

aauuacgaug acaaccgccu ucgggaaaag aagcuguuuc uguauuucau gcagcucggg     2520

aaaagcaugu acaccggaaa gaauauugau cugaacgagc uucuguuugc caauaccuac     2580

gacaucgauc acaucauucc ccaguccaaa cucaaggacg auagcuucga caacaucgug     2640

cuggucgaga agacaauuaa uaaagacaag gauaacagag acuaucugga ugaaucuauc     2700

cggcuucgca ugaauaaauu uuggaacuuc cucagagaga agggcuucau uaccaucgaa     2760

aaauacaauc ggcugagacg caacacauau uuuuccgacg augagcugag cggcuucauc     2820

ucucggcagc ucgugacgac cagacaaucc acacuggaag uuaagaaacu uuuugaguuc     2880

auuuuucaga acaccgacgu ggucugcagc aaagcuaauc uggugucuga uuuccgcgag     2940

aaguacggga ucauuaaagu gagagaccuc aacgaugccc accaugcuca ggacgccuau     3000

cugaauaucg uugucggaaa cguguuugau acaaaguuca ccaaaaauua cuggaaguuu     3060

auugaagacc ggcgcaaggu ggacaacauc agagagacgu auaauuucaa aaagauuuuu     3120

gauuacgacg ucagccgggg cgaaaacgug gccuggcuga aagauuacuc caucaauacc     3180

aucagcgaga ugauuaacaa gaaaacagcu aauaucaccc aaaugauuac agaagggaag     3240

ggacagcuuu ucgaccugac cauccucaaa aagucuaaga uugauaaaaa gcugacgguu     3300

aucccuaaca cacccaaaau ccucaagucc agccugaaag acuccuguaa gauugcggag     3360

aaguauggcu acuuuucuac ccuuaugccu gccuauuucg uggucuucaa auacaucgau     3420

aagaauaaag agguguuugg guucgaccgc auuuuuaucu ucgauagaga aaagauuaac     3480

agcaaugagg acaucgaaaa auaucuggag gauaagggcu acaacucccc caaguuuauc     3540

cggaaaguua accucaagca gaaaauucug cugaauggau auccguaccg caucgugggc     3600

uucacagaca acaaacuuga aauuaaaaau ucuaagcagu uuuacagcga uggggagcac     3660

cugaaguaua ucaaacaucu cuccgcuuuc uacaacaaua acaauaacgu caagaaugga     3720

gaguauagaa uuaacuuucu gaaaaagacc ucugaaaaug accggaacga gaccacagac     3780

gaagccaaag agcgccucaa uaucgaguuc auuaagcugu acgaauauuu uugcgagaga     3840

cuuaacagca aaaucuacgc caauuauuac aacuccaaga aguacgauga acugaucgag     3900

guguucgacu guuuuaaaaa ccucaaucug cuggauaagg cuaaacuucu gcucgaguuc     3960

cugaagauuu ucaaaaagga cacguauggc aacuuuaagc uccuggaaaa accugagcuu     4020

gauaagaaug ggaaacaaaa gcuggacaaa aacggaaauc ugagcuucga aggccucucu     4080

auggccuuug ggaagcugaa cggcaagaaa uucaagcagc uuguugagaa agauauccag     4140

uuuguggacg aauccgucac cggacuguuc gagaagaaag aaggccucga gaaguggcag     4200

gauggggagc aauga                                                      4215


<210>  4461
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4461
auggccaaga aaauccuggg ccucgaccug ggggugagcu ccauuggaug ggcuaucguc       60

gaggaagaug ccgagaacgu uaugaauaac aagauuguga aacuuggcgu gagagucaau      120

ccccugaccg uggacgaaca gaucaacuuc gagaagggga aaccuauuac aacgaaugcg      180

ggacggaccc ucguucgcgg cgcuagacgg aaccugcaac gcuuuaaacu uagacggcug      240

caccucaucg auauccugca gaagcgcaau auucugaaac aggccgaccu ccuggccgaa      300

ucugggagaa acagcacauu ccagacccaa gagauccggg cuaaguccgc caaacagaag      360

guggagcuuu cugaacuggc ucgcguccuc cugcuuauua auaaaaagag aggauacaag      420

agcucccgga aagccaagaa cgaggaugaa ggccagaucg uggacggcau gucuguugcg      480

aaaaagcugu augaggagaa uacaaccccg ggggaauaca gcuaugagcu ccugaaaucc      540

ggaaagaaac agcugagcga uuuuuaccgc ucugaccucc aagccgaauu ugauagagug      600

uggaacuuuc agaagcaguu cuaucccgag auuuucuccg acaaucugua caaggaacuu      660

cagggcaaaa acaagaaugc uacauggaaa auccuggaga uuccuuucag ccucgucggg      720

aucaagcaag ugggaaccac gcaggagaaa aagaucgaaa aguauuuuug gcggucugag      780

gccgugaaaa agcagcugga uuucgaaucc cuugcugucg uuuuucaaga gauuaacagc      840

aaccugaaua acucuuccgg cuaccucggg gccaucagcg accgcuccaa agaacuguac      900

uucaauaacc agaccguggg agaguaucug cauugccagc ucaaggagaa uccccacaca      960

aaacugaaga accagguguu uuacagacaa gauuaucuug acgaguucga aaagauuugg     1020

gaaauccagu cuaaauacca uaccguccug acaaaggagc ucaaagaaga gauucgggac     1080

gugaucaucu ucuaucagag aaagcugaaa agccagaagg gccuuauuuc caucugugag     1140

uuugaaaauc gcgagauuga aaucaccgag aacggcaaga uuaaaaagaa agagguuggg     1200

cugaaggugg cgccuaaaag cucucuccug uuccaagaau uuaagaucug gcagguccug     1260

aauaaccucc aguuccagaa uaucgagucc aaagaaauuu uuccccugga ucuugacugc     1320

aaggagagca ucuucaacga agugaauauu aagggacgga ucucugccaa agcuguucug     1380

gaugugcucg gcuacagcgg gaaggagugg aaaacgaacu uuaaggacau ugagggaaau     1440

aacuccaacg aaaaucugua uaacgccuac cugaaaauca uugcuaauga gggcaucgag     1500

uucccuaagg aauuuaagug uaaccuugau gacgauauca augucucuaa aauuaucugc     1560

agcgccggga agauuaucga guucgugaga gaaaaauuuu ccgagcucgg aauuaacaca     1620

agcauccugg acuucaauuc ugaacuggag ggcaccaacu uugaaaagca aaaauccuac     1680

cagcucuggc aucugcuuua uagcuacgau ggcgacgauu cuccguccgg gaaucagaag     1740

cuguaugagc uccuggaaaa gaaauucgga uucaagaaag agcacagcau cauucuggcc     1800

gacaucggcu uuccccagga guacgggucc cuuucuacaa aggcuaugcg caaaauuauc     1860

ccucaccuca agaccggacu gcccuucgcg aagaaaagcg acaacgauac cauucugucc     1920

gccacagcua uggccggcua uaaucacucu aagagccuca cgaccgagga acugacaaaa     1980

cggguccuua aggaacaucu ggagauccuc ccuaaaaacu cccugagaaa ucccgugguu     2040

gagaagaucc ugaaccaaau gauuaaugug gucaacgaag ugaucaaaga guacgggaag     2100

ccggacgaaa uucgcaucga gcuugcccgg gaacucaaga aaaacgcuga ggagagagcc     2160

aauaugacca gcgaaauugg caaggcgaca auccagcacc agaaauauuc ugagauccug     2220

caaaaggaau uuggaauuaa auccccuagc cgcaacgaua ucauucggua caagcuguac     2280

cucgagcugu cuaauaacgg cuucaaggac cuuuauaccg auauccagau uaaaagagag     2340

gaacuguuua cggacaagua cgauaucgac cauauuaucc cccagucccg cuucuuugau     2400

gacagcuucu cuaauaaagu ucucgugccu agaggggcua accugaagaa aggaaaucug     2460

acagccuuug auuaucuuga gagcgaaggc aaggacaccc ucgagaaguu caucaacacc     2520

auuacagaau uuuacgauaa agggaucauu accaaggcca aauaugagaa gcugcagaaa     2580

aagggaacag aaaucggcga cggguucauu gagcgggacc ugcgggauac gcaauacauc     2640

gcuaagaaag ccaaggagau ccucuuuggc auuaccaauu ccgucgugag cacaucugga     2700

cgcaucaccg acaaacugag agaagauugg aaccuuguga auacaaugaa ggagaugaac     2760

cuggaaaaau aucggaagag aggccucacc gagacgguua uuaauuccaa gggggaggaa     2820

aaagagaaga ucauugacug gagcaaacgc aacgaucacc ggcaccaugc uauggacgcc     2880

cugaccgucg cguucacaac ccacaaucau auccaguacc ugaacuaccu uaacgccaga     2940

aagaaugaag aucacaaaca gcauaaggug aucuccaaca uugagaaucu gaucacaaaa     3000

guguucgaaa agaagaacgg aucuaaacag cgcagauuuc ucgagcccau ucacaauuuc     3060

cggaucgacg cuaagaaaca ccuggaggaa auucucauca gccauaaggc caaaaacaag     3120

gucgugacca agaauauuaa caaaaucaag aaaaagggcu ccaucauugc uaaaaucgag     3180

cugacgcccc gcgggcaacu ucacaaggaa acaauuuaug gaucuagcaa guaccugaaa     3240

accaaggaug agaaacucuc cggcaaguuu gaccuggaaa caaucaauaa aguucagaac     3300

gagagauaua agaaugcccu gcuugagcgg cugaaggugu ucggguacga uucuaaaaag     3360

gcguuuaccg gcaaaaacau ucucgccaag aauccuaucu uccugaacau cgaaaagacc     3420

gagcagcucc cggaaguccu gacacuugcu ugguaugaga cgauuuacac cauccgcaag     3480

agcauuaauu ccgacaacuu uaaagauuau aagaauaucg acaaggugac agacaaaaag     3540

guucauaaca uucugaccga uagacuccgg gaguucaacg gaaauagcaa agaagccuuu     3600

ucugaccugg agaagaaccc caucuggcug aauaaaccua agggcaucgc uauuaagaca     3660

gugaccauca cgggggucaa caauguggaa gcccuucacu acaaaaagga ucacuucgga     3720

aaagaguuuc uggacgaaaa gggccauaaa auucccguug auuucguguc cacagggaac     3780

aaucaccaug ucgccaucua cgaggacacc gcuggaaacc uccaggagcg cgugcugagc     3840

uucuaugaag cgguggagag agucaaucaa ggccucucug uuauugauaa ggaauacaac     3900

uccagccugg gguggaaguu ucuuuucacc augaaacaga augagauguu ucuguuccac     3960

ucugaagacu uuaacccuca ggagaucgau cuguaugacg agaagaaucu ccggcugauu     4020

uccaaaaacc uuuuccgcgu gcaaaaguuu acagugaaag auuacuucuu uagacaucac     4080

cuggaaacca augucgagga caacagcaag cucaagggcg acacauggaa acgggaagga     4140

cugaacggca ucagagggau ccucaaggug cgccugaauc accuuggaaa aauuguucag     4200

gugggcgagu auuga                                                      4215


<210>  4462
<211>  4215
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4462
augagcaaga aaauccuggg ccucgaccug gggaccacau ccauuggaug ggccuucgug       60

agagaggcug aagauuuuca ggaggaaucu agcaucgagc ggauuggcgu ccgcguuaac      120

ccccuuacga ccgacgaaca aaucaauuuc gagaagggga aaccuauuuc cgugaacgcc      180

gauagaacac ugaagcgggg agcgcgcaga aaucuccagc gguacaaaca gcgcagaacc      240

aaccugaucc agcuucugaa gcaauauaag aucauuaaug agaacacaau ccucgcugaa      300

gacgauaaaa auaccacaca cucuaccuac gcccugcggg ccaaggcugu gagcgagaaa      360

auuuccaagg aagccuuugc ucgcguccug cucaugauca acaaaaagag aggcuauaag      420

ucuagccgga aagccaagaa ugaggacgag gggcaggcga uugauggaau guccguggcc      480

aaacgccugu acgaagagga caucacgccg ggccagcuuu gccugcagcu ccugaaggaa      540

ggcaaaaaga aacuucccga uuucuaucug agcgaccucc aagcugagcu ggaaaagauu      600

uggaacuuuc agaagaaauu cuacccugag auccugaccg augaguuuaa gaaagaaauc      660

gaggggaagg gacagagagc cacaucugcu uccuucuggg ccaaauauaa guuuaauacc      720

gcggaaaaca agggcagccg ggacgagaaa aagaugcagg ccugggaaug gcgcucuaaa      780

gcuauuaaua cacaacucac cagagaggag guugccuacg ugaucacgga aauuaacaau      840

aacaucaaca auuccagcgg guaccuggga gcuauuucug aucgguccaa ggagcuuuau      900

uucaacaaag aaacagucgg cgaguaccug uuuagacagc uccgcuauag cccccauacc      960

cgggugaaga aucagguuuu cuacagacaa gacuaucugg acgaauuuga gacaaucugg     1020

gagaaccagu cccgcuacca caaugaacuu accaacgagc ugaagucuga aauccgggau     1080

guggucauuu ucuaucagag aaaacucaag agccagaaag ggcugaucuc cuucugugag     1140

uuugagagca agcaaauuga aauccagaaa gacggaaaga uugugaagaa aaucaucggc     1200

cugaaggugu cuccuaaauc cagcccccuc uuccaggagu uuaagauuug gcagauccug     1260

caaaaucuug aguucaccaa caaagagaca aaggaaaaga aggucccuga uuuugaggcc     1320

aagaaucucc uguucgagga acugaacauu aaaggcaauc uuaaggggga gaaagccauc     1380

gaaauucugg gauacaagcc gaaagagugg cucguuaacu acaaggaacu ggacggcaau     1440

cgcacgaacc aggugcugua ugagggguac cucaagaucc uggaggugga aggauaugau     1500

auuaaagacc uucugaagau caaaucuaau aaggaugagg ucaaccucag cgaacugucc     1560

aaacccgugu cugagaucaa ggaccugauu aagagcaucu uugaaucccu uaacauuaau     1620

accgcuaucc ucgauuucaa cccugagcug gagggcaaag acuuugaacg gcaggcgagc     1680

uaccagcugu ggcaucuccu guauucuuac gagggggaua auuccaacac aggaaaugac     1740

gaacuuuaua gacugcucua ccaaaaguuc ggcuuuggca aagagcacgc ccagauucug     1800

gcuaaccuga gcuucucuca ggacuacggg ucccuuagcg ccaaggccau gcgcaaaauc     1860

uaucccuuua ucaaggaaaa uuccuacucu gaggcuugca gccucgccgg auauaaccau     1920

uccucuagca uuaccaagga agaaaaugau aaacgggagc ugaaggaaaa acuggagcuc     1980

cugccuaaga acucccuuag aaaucccguu guggagaaaa uccugaacca gauggucaau     2040

gugauuaacg cgaucauugc ugacccgagc augggccgcc cugaugaaau ccggaucgag     2100

cucgccagag aacugaagaa aaaugccgcu gagcgggccg aaaugacaau ucacaucaac     2160

aaggcuaagu cugagcacga caacuaccgc aaaauucuga ccacggaguu cgggaucccc     2220

aauccuacaa gaaacgauau uauccgguau aaacuuuacg aggaacucaa aguuaaugga     2280

uauaagaccc uguacuccaa caccuacauc cccagagaaa aacuguucag caaggaguuu     2340

gacguggaac auauuauccc caaggcccuc cuguucgaug acucuuuuuc caauaaaaca     2400

auuugugcgc gccaagagaa ccuugauaag ggcaauaaaa ccgccuucga cuuuauccag     2460

agcuucuaug ggaaggagaa auuugaagag uaccugcagc ggauugaauc ucucgcuaag     2520

aucaaggagg gcgauacauu cgucauuagc aaagccaagu auaaaaagcu gcugcuuccu     2580

gagaucgaaa ucggagaggg cuuuauugaa agagaccucc gcaacuccca auacaucgcu     2640

aaaaaggcca agagcaugcu gcuggauauu ugcaaaacgg uggucccgac cacagggucu     2700

aucaccgaca gacuccggga ggacugggga cugauuaaua ucaugcagga acuuaacuuc     2760

gagaaguaua aaaagcuggg ccucaucgag augguggaga aaaaggaugg guccuuuaag     2820

gaacgcauua cagacuggac caaaagaaau gaucaccggc aucacgcgau ggacgcccug     2880

acgguugcuu ucaccaagca caaccauauc caguaccuga auuaucuuaa cgcccgcaaa     2940

aaugaaaacc acaagaugca uggaaacgug auugcuaucg agcagaaaga aacagagcug     3000

cucgucgaua gcgugggcaa gagaaaacgg aaguuuaagc ugcccauuuc caauuuccgc     3060

gagguugcca aaaugcaccu cgaaaacauc cuggugucuu acaaggccaa aaauaagguc     3120

gcuaccaaaa acaagaauag auucaagacc aaaaacgggg agaaggugaa aaucgaacuu     3180

acaccucggg gccaacugca uaaggagacg guguacggaa aaaugaagaa guaugaaaca     3240

aaagaggaga uuaucggcag caaguuugac gaagcgacca uucucaaagu ugccaaucug     3300

gagcagcgca acgcucugcu ucagagacug aaggaaaaua acaaugaucc caaaaaggcc     3360

uucuccggga agaacucucu caaaagcaau ccucuguacu auggagucga cuccaagccc     3420

aucccggagc ggauuaaaau cguguggcuc gaggaugacu acacaauuag aaaggaaauc     3480

accccugauc ugaaaaucga caaggugauu gauaucggca uucgccggau ccuugagaac     3540

agacuggccg aauuuaaugg gaacgcuaaa gaggccuucu cuaaucucga aaagaacccc     3600

auuuggcuga acgagggaaa gcgcaucgcg aucaaacggg ucaccauuag cggcgugaag     3660

aaugcugaga cacugcacua uaaaaaggac caccuugggc augaaaugcu ggacaaacag     3720

ggcaagagaa ucccuguuga uuuugugucc acgggaaaca aucaccaugu cgccauuuac     3780

aaggacgaua aaagcgaccu ccaagagaag gugguuucuu ucuaugaagc cguggagcgc     3840

aucaaccagg aacugcccau uaucgauaaa uccuuuaauc acgaccucgg cuggcaguuc     3900

cuguuuacca ugaagcagaa cgaguacuuc gucuuuccuc gggauggguu cgaccccuac     3960

gagaucgauc uucugaaucc ggacaacuau aaaauuauca gcccuaaucu guucagagug     4020

caaaagauug cuacaaagaa cuacguuuuu aaucaccauc ucgaaaccaa agccguggau     4080

ggagagcugc uuaagaccaa aaaggaccug ucugacauca cauaucgcuu cauucagacg     4140

cccgucaacc ucaaaaauau cauuaaggug cggaucaacc acaucggcca gauugugaag     4200

aucggggaau acuga                                                      4215


<210>  4463
<211>  4218
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4463
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc ccuugugaaa       60

gaggcugaaa auuccaacga gaagucugaa aucauuaaac ugggcgucag aguuaauccc      120

cucacggugg augagaagac caacuucgaa gccgggcggc cucugagcac aaaugcggac      180

cgcaccgcua aaagauccgc ccggcgcaac cuucagagau acaagcugcg gcgcaagaau      240

cucaucgauc ugcugauuaa aaaccaacuc aucgacaagg auacaccgcu gaccgagauc      300

ggcaaaaaua caacccacca gacgcuugag cugagagcca aggcugcccg ggaacgcauu      360

gagcucgaag accuggcuag aguguuucuu gccaucaaca aaaagcgggg auaucgcucu      420

agcagaaagg ucaauaacga ggaggaaggg caggcggugg auggaauggc cguugcuaaa      480

aagcuguacg acgagaaucu cacccccggc caguaugccu acgaacugcu guccaaaggc      540

aagaaauaug ugccugauuu cuaccggucu gaccuccaag cugaguuuaa cgccauuugg      600

gaauaucaga agcaguucua cgcggauauc cuggacgaug agcuuuacaa ugcccugaaa      660

gggcagggac aacagaacag ccgcaagaga uuucucgcua uuaagggcgu cuauacagcc      720

gagaacaaag ggaagcggga cgacgugaaa cugagacauu acaaguggcg cuccgaagcu      780

aucagcaaaa agauuaauau cgaggaaguu gccuaugugc uugucgagau caacaaugau      840

cugaacaagu cuuccggaua ccucggcgcc auuagcgacc ggucuaaaga acuguauuuc      900

aauaaggaga ccguggggga gaaccugugg aaacagaucc agaagaaucc ccacacaucc      960

cucaaaaacc aaguguuuua cagacaggau uaucuggacg aguucgagca gauuugggaa     1020

acccaagcuc aguuucaucc ucagcuuacg cuggcgcuca aggagcagau ccgcgauguc     1080

auuaucuucu accaacggaa gcugaaaagc cagaagggac uucugucuuu uugccaguuc     1140

gaauccuggg agaucgagag aaaagacgaa aauggcaacg uuauucucaa uaagacaacc     1200

cagcugccca aacgccaaac agugggccgg agaguggccc cuaagagcuc cccgcuguuu     1260

caggaguuca agaucuggca gaacauuaau aaccucgaag ucaccaaaau cucugagggg     1320

gcuagcaaga auaaaaagga gaccgaaaca cuguccgaug acgagcgcaa acuucuguuc     1380

gaagaacuca accugcgggg aaaccugagc cagaaggaag ugcuucaaau ucucggccug     1440

aaagauaagg aguacaagac gaauuuuucu gaggggcugg aaggaaacag aaccaaugcc     1500

gcccucuuca acaucuauca gcagaucgcu gagaaugaag gcuacggguu ugacuggucc     1560

aaaaagagcg cccaggagau uaaagaagaa cugaaggcgg uuuuccccca aaucggcauu     1620

caggcugaua uccuugacuu uaacgccaau cuggacggaa aagaguucga aaaccaggcc     1680

ucuuaucaac ucuggcaucu gcuguacagc gcugaggaag augacaagau uaaugaggaa     1740

gaucagauca uuuauggcaa cuccgccgug ucucuuaaga aaaagcucug ugagaaauuu     1800

ggguucacac cugaguacgc uaaguggauc gccaauguca gccugcagga cgauuaugga     1860

aaccugucca ccaaagcgau gcgcaagauc auucccuacc ucaucgacgg caaugauuac     1920

agcgaagccu gcgcucuggc cggguauaac cacucuaauu cccuuacaaa ggaggaaaac     1980

gacaaccggg agcugcucga uaaacuggag cugcuuccua agaauagccu cagaaacccc     2040

gugguugaga aaauucugaa ucagauggug aacgucguga aucaagucau cgagaccuac     2100

ggaaagccgg acgaagugcg gauugagcug gcucgcgaac ucaaaaaguc ugccgagaag     2160

agagcggaga ugacgaaauc caucaacgaa gccacacgga gaaaugagga uaucaagaaa     2220

cugauuacca aggacuuugg caucccuaac cccaccaaaa gcgauguugu gcgcuaucgg     2280

cuuugggagg aacuggcucc ucucgccuac aaggacgucu ucacagggaa acagauuaag     2340

aaggaagacc uguuuuccuc uaaaaucgau auugagcaca ucauccccaa ggcucugcuu     2400

uucgacgaua gcuuuuccaa uaaaacccuc guguucagag agacaaaccu gaagaaagcc     2460

gaccgcacgg ccuaugauuu cauugaaucu gacuacaaug cuacccugga ugacuauauc     2520

cagagaguug agacacucua caacaauacc aagggcacaa uuagcaaggg aaaacggaac     2580

aagcugcuua uggcgcagaa aaaucugccc gauggcuuua ucgaacgcga ccucagaaac     2640

ucccaauaca uugccaagaa agcuaaggcc augcuguauc agguguucaa ggaugucaac     2700

gugaccagcg ggucuaucac ggaccggcug cgcgaggauu gggaccuuau caaugugaug     2760

aaagaacuga acuuuccuaa guacaaagcc cucggacuga ccgagauuga ggaaagauau     2820

gacaucggcc aggagaagac aaaacggguu gaagucauua ccgauuggac aaagcgcaau     2880

gaccacagac aucacgcuau gaacgcccuc accguggcgu ucacgacauc cagccauauc     2940

caguaccuga auuaucuuaa cgcucggaag aaugagucuc acaaagagca caagaacauu     3000

aaagggaucg aaaauaagau uacccauaaa caaaaggaua agcgcaaauu uaucccgccc     3060

augccuaacu ucagagagga agccaagaaa cacaucgaga caauucuggu guccaucaag     3120

aauaaaaaca aggucuacac caaaaauauu aacaagacca agaaaaagag cggacauaau     3180

acaaaagaac agcucacgcc ccggggccag cugcacaagg agaccgugua ugggaaaucu     3240

aagagaccua ugagcaagcc cacaaaaauc aacaagaauu uuucccugga gcaagcccag     3300

cuuauuauca acguucagga aaaagagcug gugcuccgcc aucuggcuca guucgacaac     3360

aauccggccg ucgcguuuga uaccaagaca cucaaaaaga ugccucugcu uaagaacggc     3420

gaaccccuga aagaggugcu cuguuucgaa gagaucuuua ccauucggaa ggacaucacg     3480

ccugaucuga aaauugagaa gguuguggac gagaaaauca aggcuauucu ggaacaaaga     3540

aagaaagauu ucggaggcaa ugccaaggaa gccuuuagcg accuugauaa aaaccccauc     3600

uggcugaaua agaaaaaggg gaucugcauu aaacgcguca caaucaccgg agugucuaac     3660

gcugaggccc uccaccacca gaaggaccau cugggccggg aaauucucga cgauaauggg     3720

aacccuaucc ccgcugacuu cguuuccacc ggaaauaacc accaucuggc cguguaccuu     3780

gauagcgagg gcaagcugca ggacaauauu gucuccuucu acgaggcggu ggugagaaca     3840

ucucaggggc ugccggucgu ugauaaaggc cucaacaagg accugggaug gaaauuucuu     3900

uucagccuga agcaaaauga aauguuugug uucccuaacg augacuuuga uaucaaugac     3960

uucgaucucc uggacgauaa aaaggccgag cucauuucca agcaccugua ucgcgugcag     4020

aaaaucucua aggucagcgc uggcaacucc uuugugcggg acaucguuuu cagacaccau     4080

cuugaaacca cagugaaaga ggucaaggag cugaaagaca uuucuuacau ccagcucaag     4140

agccuggaag ggcugcgcaa cauuaagaaa gugcggauca aucacauugg aaagaucguu     4200

caggugggcg aguauuga                                                   4218


<210>  4464
<211>  4221
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4464
auggagaaga aaaucauugg caucgaccug ggggugacca gcauuggaug ggccuacguc       60

aucgaagaug agaacggcca cucccggauu cucagaaucg ggacacgcgu uaauccccug      120

acgaccgagg aacuuaagaa cuucgaacag ggaaaaccua ucacagagac cgcugagaga      180

acacugaagc ggucuaugcg cagaaaucuc caacgguaua aacagcgcag aaccaaccug      240

auuaaugugc uucugaagaa caagcucauc acagaaacca cggcccuggc ggaggacggc      300

aaagggacca cacaucugac ccacgcucuc cgggccaagg ccgcuuacgc ccagauuccg      360

cuggaagauu uugcucgcgu gcuucugaug cucaauaaaa agagaggaua ucggagcucc      420

cgcaaaguca agacagaggc cgacggccag gcgaucgaug gcauggccgu ggcuaagaaa      480

cuguacgagg aaaacauuac ccccgggcaa cuuugcuaua cgcugcuccu gcagggaaag      540

aaaacacugc cugacuucua cagaucugau cuccagguug agauggaaaa gguguggacc      600

uuucagaaac aauuccauca ggccauccug acagacgagu uucggaagua uauugcuggc      660

aaagggcagc gcgccaccgc ggccagcuuc uggggaaagu acggcuuuaa uaccgcugag      720

uucaaggggu ccagagaaga gaaaaagcug aaaucuuacg aauggcggag ccaggccauc      780

acaacgcccc uuucccaaga ggaagucgcu gccgugauca ccgauauuaa cagcaauauc      840

gccaacucuu ccggauaucu cggcgcuauu agcgacagau cuaaggagcu guuuuucaau      900

aaacagacag uuggccagua ccaauauaac cuucugcagc agaauccuca caccaaccuc      960

uccggacagg uguuuuaccg ccaagauuau cuggacgagu ucgaaacaau cugggagacc     1020

caggcgacgu uucauacaau gcugaccccc agccucaagg aagagauucg ggauaucguc     1080

aucuucuacc agagaaagcu gaaaucucag aagaaacuug uguccauuug ugaguucgaa     1140

aagcgcaucc uggaaccucu ccaaggggac aaaguggucg gcuauaaggu ugcccccaag     1200

agcuccccgc uguuucagga guucaaaauu uggcaggcuc uugggcggau ggagauccag     1260

cacaagguga ccaaagaaag acacuuucug gaccucgaug ccaagcaacu gcuguucgac     1320

gagcucaacg ugaaaggaga ucugacaaag gaaaaugccc uuaccauucu gggcuuuaag     1380

ccuaaagagu gggagugcaa ccuccugaag cuggaaggga aucgcacaaa ccaggcucuu     1440

uacgaggccu accucaaaau ccuggaacug gagggauaug aagucaagga ccuccugggc     1500

cuuaaacugg auaaggacga uaucaaacuc ucugagcugc ccguggacgc ggcugauauu     1560

aagaagaugg uucgggagau cuucgaaacg cugaauauua aaaccagccu ucucgacuuu     1620

aacgccacac ugccuggcaa guccuucgag cagcagcaug ccuaccaacu guggcaccuc     1680

cuguauagcu acgaagggga uaauucuccc uccggaaaca aaaagcugca ggaccuucuc     1740

caggcuaaau uuggcuucac caaugcccau gggcaaaucc uggcuaacgu gagccuggag     1800

acagacuaug gaucucuuag cgccaaggcg auuagaaaga ucuuuaccca cauuaaagaa     1860

aaucacuacu ccgccgcuug ugcccucgcu ggcuauaacc auucuagcua ccuguccaag     1920

gaggagaaug aacagcgcau ccugaaagau cggcucacga uccugccgaa gaacagccuu     1980

agaaauccug ucguggagaa aauucugaac cagcucauca accugcugaa uaccauuuuc     2040

gacgaucccu accuugggaa gccugacgaa auccgcauug agcucgcccg ggaacugaag     2100

aaaaacguuc aggagagaca acggcugaau acagagauca ccaagaacaa uacagaacac     2160

gagcgcauca ccaaaauucu caucaaggaa gauaacauua aaaaucccac gagaaacgac     2220

aucauucggu auaagcugua ccaggagcuu aagcauaaug gcuauaaaac acuguacucu     2280

aacaccuaua uccccagaga ggaacucuuu ggaaagaauu ucgauaucga gcacauuauc     2340

ccucagucca uucuguucga cgauagcuuu ucuaacaaaa cacugugcgu gcgcaaggaa     2400

aauaucgaca aagcggaucg gaccgccaug gacuucauug gcccguacuu uggggaggau     2460

aagguccagg aauauaucaa cagaguggag augcuucauc aaaucaaucu gaaaaacccc     2520

gaggaaggaa uuggcaaggg gaaguacaaa aagcuccuga ugaaggcuuc cgagaucgga     2580

cagggcuuca uugaacgcga ccucagagac acccaguaca ucgccaagaa agcucggcag     2640

augcugcuug aggucacacg cacggugguu gccaccacag ggagcauuac caagagacug     2700

cgggaagauu ggggccucgu gaacgucaug caagagcuga auauggacaa guaucgccag     2760

cagggacuga cagagcuuau cgaaaagaag gauggcucca ccaaagagag aauuacggac     2820

uggacaaagc ggaacgauca ccgccaccau gccauggacg cucugaccau cgcguuuacc     2880

aaacacucuc auauccaaua ccucaauaac cugaaugcca gaaagagcga ugacaagaaa     2940

gggaaggaaa uuuucggaau cgagcagaaa gaaacagaga aggugaaaga ugacaccggc     3000

cagacacagc ggcgcuuuaa gcucccuaug cccgauuuca gacaagcugc caagcaccac     3060

cuggagcauc uucugauuuc cuuuaaagcc aagaacaaag uuuucacgaa gaauaaaaac     3120

aaguauaaga ccaaaaaugg ggaacaggug aagcucgagc ugacaccucg gggacagcug     3180

cacaaagaaa ccaucuacgg cagaauuaag aaauauguca cacaugagga gaaggugucu     3240

agcaaauuua acgcucagcu uauccaaacc guggccaauu ccaaguaccg cuaugcgcug     3300

cucaagcggc uggaagagaa cgggaaugac cccaaaaagg cuuucacggg caaaaacagc     3360

cucgauaaga ccccgcuguu uguugacgcc acacagcagg agaaaauccc ucccgucgug     3420

aaacuugugu cuauugaaca gggauacacc aucagaaagg acauuacacc ugaaaauuuc     3480

aaagaugcca aguccaucga gaaaauuguc gacgugggca uccgcaaccu gcugcaccau     3540

cggcucgaga ccuacaacgg gaaugaaaag gaggcuuucu auaaccugga uaaaaauccc     3600

aucuggguua acgaaagcaa gggaauuugu aucaagagag ugacaauuac cggcgucacg     3660

aaugccgagc cucuucacua caaacgcgac caccauggga agcccauccu ggauaacaau     3720

ggaaaccgga caccggcgga cuuugugucu accggcaaua accaccaugc ugccauuuau     3780

agagaugccg cugggaaucu ccaagacaac guuguguccu ucuacgaagc cgucgcucgc     3840

gugaaucagg gccugccuau cauugauaaa agccucaaca aggagcuggg auggcaguuu     3900

cuuuucucuc ugaaacagaa ugagugcuuu guuuucccca acgaaggcuu ugacccccug     3960

gagaucgauc ucuauaaucc ugccaacaac aagcugaucg cggccaaucu uuuccgggug     4020

caaaacuuua gcagacugca guacgggaau aacauuaucc gggacuauaa uuuccgccac     4080

auuuacgaaa ccuccgucga gaacagaaag gaacucaaag acaucgcuua caaacaggug     4140

aaaagccugc cgauuuuuga gaagaucauc aaagugcgga uuaaucauau cggacaaauu     4200

gucaagacag gcgaguauug a                                               4221


<210>  4465
<211>  4224
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4465
augaucagaa acauucuggg ccucgaccug ggggugagcu ccaucggaug ggccuacguc       60

caggaggauu cuaagaauag cgaaaacaau cggauuauca aacuuggcgu ucgcgugaac      120

ccccugaccg uggacgagca acucaauuuc gaaaagggga aaccuauuac aacgaacgcu      180

ggaagaaccc uggcccgguc cgcgcgcaga aaucuucagc gguuuaagcu gcgcagaucu      240

aaccucaugg auguccugaa aaagaauaac auccugaagg agacagaccu ccuggcugaa      300

gugggcaaaa auagcaccuu ccagacacag gagcuucggg ccaaguccgc caaagagaag      360

aucgaacugu cugaguuugc ucgcguucuc cugcuuauua acaaaaagag aggguauaag      420

agcucccgga aagccaagaa ugaugaagac ggacaaaucg uggauggcau ggcugucgcc      480

aaaaagcugu ucgaggagaa ccucaccccg ggcgaauaca gcuaucagcu gcugcagcag      540

gggaaaaagc aacuccccga cuuuuaccgc ucugaucugc aggcggaguu cgacaaaauu      600

ugggauuuuc agaagcaguu caauccugaa aucuuuacaa acgagcuuua ugaaaagcug      660

caaggaaaga acagaaaugc caccuggaag gagcucgagu uccccuuuuc ccuggugggc      720

auuaaacaga cggggaccau gcaggaaaag aaaguugaga aguacuucug gcggagcgaa      780

gcugugaaga aacaacuuga cuuugagucu cuggccgucg uguuccagga aaucaacucc      840

aaucucaaca auagcucugg auaucugggc gcuauuuccg aucgcagcaa ggagcuguac      900

uucuacaacc agacaguggg ggaguaucuc uuucagcaac ugaaaaccaa uccucacaca      960

aaacuuaaaa accaggucuu cuacagacag gacuaucugg acgaauuuga gaagaucugg     1020

gaaacccagu ccaaguacca uaaagagcuc acgaaggaac ugaaagagga gauccgggau     1080

auuacaaucu ucuaucaaag aaaacuuaaa ucucagaagg gacugauuag caucugcgaa     1140

uuugagaauc gcgaaauuga gaucaccgag aacggcaaga ccaaaaagaa aaccguuggc     1200

cucaaggugg cccccaaauc cagcccucug uuccaggaau uuaagaucug gcaagugcug     1260

aauaaccucc aguuccagaa uauugagacc aaagaagucu uuccgaucga ccuggauuuc     1320

aagcagucua uuuuugacga ggugaacauc aaggggaaac uuuccgcgaa ggauguucug     1380

gaccucgugg gauacagcgg caaagaaugg aagacaaauu ucaaagagau ugaggggaac     1440

uauacgaacg aaaaucugua caccgccuuu cugaagauca ucgcuucuaa ggagauugaa     1500

uuucccaaag aguucaagau cacaauugau gacgaaauca aagucagcaa gauuaacgcc     1560

uccgcugaga ucauuaaacu uuucgugcgg gagaaguucu cugaacucgg aaucgauacc     1620

agcauccugg acuuuaaucc ugagcuggaa ggcuccgauu ucgagaagca ggccagcuac     1680

caacucuggc aucugcuuua uucuuacgaa ggggacgacu ccagcucugg aaacgagaaa     1740

cuguaugagc uccuggaaaa uaaguuuggc uucaaaaagg agaacuccaa aauucuggcc     1800

gaaaucggcu uuagccagga uuacgggucc cuuucuagca aggcuaugag aaagauuuau     1860

cccuacauca aagagcacaa guauuccguu gcguguucuu acgccggaua caaucacagc     1920

aaaaacuccc ucacaaagga gcagcuggaa aaucgcauuc ugaaagagcg gcucgacauc     1980

cugccuaaga acagccuuag aaaucccgug gucgaaaaga uccugaacca aaugauuaau     2040

cucgugaacu gccugaucga ugaguauucu gaaaaggaca aggauggcca gauugucaaa     2100

uacuucaagu uugacgagau ccgcauugag cuggcucggg aacuuaaacu caaugccgag     2160

gaaagagcca ccaugacguc cgagaucaac aaggcuacaa uccagcauca gaaauaugcc     2220

gaagugcugc aacgcgaguu cgggcugaag gcgccgagcc ggaacgauau uaucagauac     2280

aagcucuauc uggagcuugc uaauaacgga uuuaaagacc uguacaccga ugccaagauu     2340

gaacgcgaga aucucuucac cgacaaauau gauaucgacc acauuauccc ucagucucgg     2400

uuuuucgaug acuccuucag caacaagguu cuggugccca gaaaugccaa ccugaaaaag     2460

ggcaauuuua cagcuuucga cuaccuugaa aucgagggga aggaaaaacu cgagaaguuu     2520

cugaacauua ucaaagaucu guacgacaag ggcgccauua ccaaagcuaa guaugagaag     2580

cuccagaaaa aggauuucga aaucggagac ggcuuuauug agcgggaucu gcgcaauaca     2640

caguacaucg ccaaaaaguc uaaagaaauu cuuuucggga ucacgaacag cgucgugacc     2700

acauccggaa gaaucaccga caagcugcgg gaggauugga aucucauuaa cacaaugaag     2760

gagcugaauc uggaaaaaua uagaaaacuu ggccugaccg agacgguuau caacagcaaa     2820

ggggaagaga agcaacgcau uaucgacugg accaaacgga augaucacag acaucacgcg     2880

auggacgccc ucacaguggc uuuuaccaca cauaaccaca uucaguaccu gaacuaucuc     2940

aaugcccgca aggaugaaaa acacaaggag caucagaaca ucuucgcuau cgagaaucug     3000

auuaccgaaa ucauugacaa gaaaaacgga ucuaaggaga gacgguuuaa agaaccuguc     3060

aagaaucuuc gcgccgaggc gagaaaacac cuggaugaaa uccucauuuc ccauaaggug     3120

aagaacaaag ugguuacgaa gaauaucaac aaaaucaaga aaaagggcag cgucauugcc     3180

aagacagagc ugacaccccg ggggcagcug cacaaagaga caaucuacgg cuccucuaag     3240

uuccuuaaaa ccaaggaaga gaaaauuagc ggaaaguuug acguggaaac cauccaaaag     3300

gugcagaaug agcgcuauag aaacgcucug cucaaacggc ugcaggaguu cggcggggac     3360

uccaagaaag ccuucacagg aaagaauguc auuucuaaaa accccaucuu ucucaauaac     3420

gaaaagacgg agcaacugcc ugaaaccgug acacuugcuu gguacgagau gggcuacacc     3480

auucgcaaag ccguuaaucc ggauaacuuc aaggacuuua agaauaucga aaagacaauc     3540

gauaagggga uuagagacau ccugaccaaa cggcucaagg aguucaacgg aaacagcaaa     3600

gaggccuuuu ccgaucugga caagaauccc auuuggcuga acaagucuaa aggcaucagc     3660

auuaaggcug ugacgaucac agggaucaau aacgcggaag cccuucauua uaaaaaggau     3720

caccugggca aagacuuccu cgaugagaau ggaaagcgca uugcugucga cuuugugucc     3780

accggcaaca aucaccaugu ugccaucuac gaagaugaga acgggaaucu gcaggaaaag     3840

guggucagcu ucuaugaggc cguggagaga guuaaccaga aucucccuau uaucgacaaa     3900

gaauacaacu cugaguccgg auggaaguuu cuguucacca ugaaacagaa ugaaauguuu     3960

cuuuucccca gcgaggacuu uaacccuaag gaaauggauc uguucgacga gaaaaaugug     4020

aaccugauuu cuaagaaccu cuuccggguc caaaaguuua caaucaaaga uuauuucuuu     4080

agacaccauc uggagaccaa uguggaagac uccagccccc uuaagggcau cacauggaaa     4140

cgcgaggggc ugaacggaau ugaaaauauc cucaaggugc ggcugaacca ccucggcaaa     4200

auuguccaga ucggggagua cuga                                            4224


<210>  4466
<211>  4227
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4466
augaccaaga acauccuggg ccucgacgug ggggucagcu ccauuggaug ggcccugauc       60

aaugaggaua uugaaaaccc caaaaauaac aagaucauua aacuuggcgu uagagugaau      120

ccucugacag uggacgagca gaucaacuuc gaaaagggga aaccgaucac gaccaaugcu      180

ggacggacac ucgcccgcgg cgcgagacgg aaccugcaac gcuuuaaacu uagacgguuc      240

caccugauug auauccucca gaagaauaac auucugaaac aggcugaccu gcucgccgag      300

gucgggaaaa auucuaccuu ucagacacaa gaaauccgcg ccaagagcgc uaagcagaaa      360

guggagcugu ccgagcuugc cagaauuuuc cugcucauca acaagaagcg gggauacaaa      420

ucuagccgca aggcuaaaaa ugaagaugag ggccagauua ucgacggcau guuuaucgcc      480

aagaaacugu augaugaaaa acuuaccccc ggggaguacu ccuaucagcu gcucaaggag      540

ucuaagaagc aacugccuga cuucuacaga agcgaucugc aguccgaauu ugacaaaauu      600

uggaacuucc agaagcaguu uuaucccgag aauuucagcg auaaccucua caaagaacug      660

caaggaaaga acaaaaaugc gacauggaag acccuugaga ucccuuuuuc ucugguuggc      720

auuaagcaga cggggacccu ccaggaaaag aagcuggaga aauauuccug gcggagcgac      780

gccgugaaga aacaacuuga uuacgagaag cuggcugucg uguuccagga aaucaacucu      840

aauauuuaca acuccagcgg auaucucggc gccaucucug accgcuccaa ggagcuguac      900

uuuaauaacc agacaguugg ggaauaucug uacaaucaga ucaaagagaa cccccauacc      960

aagcucaaaa aucaaguguu cuauagacag gacuaccugg augaguucga aaagauuugg     1020

gagacacaga gcaaauauca caccacgcuu acaaaggaac ugaaggagga aauccgggac     1080

auuaucaucu uuuaccagcg caaacucaag ucccaaaaag gacugauuuc uaucugcgag     1140

uucgaaaaca gagagauuga gaucaaggaa aauggcaaaa uuaagaaaaa gaccgucggc     1200

cuuaaggugg cuccuaaaag cuccccgcug uuucaggagu ucaagaucug gcagauucuc     1260

aacaaucugc aguuucaaaa caucgaaagc aaagagauuu uccccaucga ucuggacuuu     1320

aaggagucua ucuucaauga agugaacauu aaagggcgga ucuccgccaa ggcgguccuc     1380

gaucugcuug gauacagcgg caaggagugg aacccuaauu uuaaagacau ugaagggaac     1440

aauacaaacg agaaucugua uaacgccuac cucaagauca uugcugauga agaguucgag     1500

cugcccaaag aauuuaagcu gaauggagac gaugaccuuu auguuucuaa aaucgccugu     1560

agcgcuucca agaucaagga uuucgugaaa gagaaguuuu cugaaauugg caucaacacc     1620

agcauucucg acuucaaucc ugagcuggaa gggaaagacu ucgagaagca ggccuccuac     1680

caguuuuggc aucugcucua uagcuacgag ggagaugacu cucaguccgg caacgaaaaa     1740

cuguaugagc uucuggaaaa gaaguucggc uuuaaaaagg agcacagcaa aauccucucu     1800

aauauugggu ucccccugga uuacggaaac cuguccacca aggccaugag aaaaaucauc     1860

ccgcaucuua aggcuggccu cagcuuugcg aaaaagucca aggaagacaa uauucugucu     1920

gccacagcua uggccgggua caaccacagc aauuccuaua cgaaagagga ucuggccaag     1980

cgcgugcuca aagagaagcu ggaagucuuc ccuaaaaacu cucuucggaa ccccgugguu     2040

gagaagaucc ugaaucaaau gauuaaccuc gugaaugaag cugucgagga guacggaacc     2100

aucgacgaag ugagaauuga gcuggcccgc gaacugaaga aaaacgcgga ggaacgggcu     2160

aauaugacaa gcgagaucgg caaggccacc cuucuccacc agaaauaugc cgagauccug     2220

aagaaagaau uugauauucc uguucccucc agaaacgaca ucauucgcua caagcuguau     2280

cucgagcugg cuaauaacgg guacaaggau cuuuauacag acgugcagau caaacgggag     2340

gaacuguuca ccaauaagua cgauauugac cauaucauuc cccaaagcag auuuuucgau     2400

gacucuuuuu ccaacaaagu ccucgugccu cgcggcgcca aucugaagaa agcuaaccag     2460

acggccuucg acuaccugga aaaugaggau aagcagaaac uugagaaguu ucucaacauc     2520

aucaagaauc uguaugacaa aggagaaauu acaaaggcga aauacgagaa gcugcagaag     2580

aaaggcagcg auaucgggga aggauucauu gagcgggacc ucagagauac ccaauauauc     2640

gccaagaaag cuaaggaaau ucuguucggc aucacccacu cugucaucuc cacaagcggg     2700

aaaauuaccg acaaacuucg cgaggauugg aaccugguga acacaaugaa ggagcucaau     2760

cuggacaagu acaagaaacu gggacuuacg gaaacccugc ucaacucuaa gggcgaggaa     2820

aaagagcgga ucauugauug gagcaagaga aaugaccauc ggcaccacgc cauggaugcu     2880

cugacaguug ccuuuaccac acauaaccac auccaguauc ucaauuaccu gaacgcgcgc     2940

aaagacgagg aucauaagca gcacaaggug auuuccaaua ucgaaaaccu uaucaccaaa     3000

gucuucgaga agaaaaaugg ggccaagcag agacgguuua uugaaccgau caacaauuuc     3060

agaguggacg cuaaaaagca ucuggacgag auucucauca gccacaaggc caaaaacaag     3120

guugugacga aaaauauuaa caagaucaaa aagaagggcu cugucguggc uaaaaccgaa     3180

cugacacccc gcggacaacu gcacaaggag accauuuaug gcuccagcaa auuucuuaag     3240

acaaaagaug agaagaucuc cgggaaauuc gaccuggaaa ccaucaauaa ggugcagaac     3300

gagaaguaca aaaaugcccu ccugcagcgg cucagagaau uuggagagga uucuaagaaa     3360

gccuucacgg gcaagaacgu uauuagcaaa aacccuaucu accuggacga agagaagaca     3420

cuucagauuc ccgaguccgu cacccuggcu ugguaugaaa ugaucuacac aauucgcaag     3480

ccugugaauc ccgauaacuu uaaagacuau aagaauaucg auaaagugac cgacgagaag     3540

aucagagcgg uccucaccga ucggcugcgc aaauucaacg gaaauucuaa ggaagccuuu     3600

agcgaccugg agaagaaccc gauuuggcuu aauaaaucca aggggaucgc uauuaaaaca     3660

gugacgauca ccggcauguc uaacguugaa gcccugcauu acaagaaaga ucaccucggg     3720

aaggagauuc uggacaagaa aggcgacaga augccugugg auuucgucag cacaggaaau     3780

aaccaucacg ccgcuaucua ugaggacgcc aagggcaauc uccaagaacg ggugguuucc     3840

uucuacgagg cgguggaacg cgucaaccag gggcugagcg ugguuaauaa agauuauaac     3900

gcuggacuug gcuggaaguu ucuguucacc augaaacaga augagauguu ucuguucccc     3960

ucugaggacu uuaacccuaa ggaaaucgau cucuacgacg agaaaaaccu gaaacuuauu     4020

gccaagaauc uguucagagu gcaaaaguuu ggggaacucu ccaagagcgg auucugguuu     4080

cggcaccauc uggagacauc ugucgaugug aaaaaggaac uccgcaacua cuccuaucug     4140

gacuucuaca gcaaagauuu uaugaagacc aucgugaagg ucagacuuaa ucaccugggc     4200

aaaauuguuc agguggggga guauuga                                         4227


<210>  4467
<211>  4230
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4467
augagcauca uuaacuucca gagacggggc cugauggaga cccaagccuc caaucagcuc       60

aucucuagcc accugaaggg guaccccauu aaagacuauu uugugggacu ugauaucggc      120

acauccucug ucgggugggc uguuacgaac aaggccuacg aacugcucaa auuccgcagc      180

cauaagaugu ggggauccag acuguuugac gagggcgaau cugcgguggc ucggcgcggg      240

uucagaagca ugcggcgcag acuugagcgg cgcaaacuga gacucaagcu gcuggaagaa      300

cucuuugccg augccauggc ucagguggac ccuaccuucu uuaugcggcu gcgcgagucc      360

aaguaucacu acgaagauaa aacaaccgga cauagcucua agcacauucu uuucaucgac      420

aaaaauuaua cagaucagga cuacuauaag gccuacccga ccaucuacca ucugagagcu      480

gagcucauga aagauggcac agacgauauu cggaagcugu uucuugccgu ccaccacauc      540

cugaaguauc gcggcaacuu ccucuacgaa ggggcgaccu uugacuccaa ugccagcacg      600

cuggaugacg ugauuaaaca agcucuggag aacaucaccu ucaauugcuu ugacuguaac      660

ucugccauuu ccagcaucgg acagauucuc auggaggcug gcaagacaaa aucugauaag      720

gccaaagcga ucgaacaucu gguugacacc uauaucgcca cagauaccgu ggacacgucc      780

agcaagacac agaaagauca ggucaaggag gacaagaaaa gacuuaaggc uuucgccaau      840

cuggugcucg ggcugaacgc uucccuuauu gaucuguucg gaucuguuga ggagcucgaa      900

gaggaccuga aaaagcugca aaucaccggc gauacauacg acgauaaacg ggacgagcuc      960

gccaaggccu ggagcgacga aauuuauauc auugaugacu gcaaguccgu guacgaugcu     1020

aucauccugc uuagcauuaa agagcccggg cugaccaucu cugaauccaa ggucaaagcg     1080

uuuaauaagc acaaagacga ucucgccauu cuuaagagcc uucugaaguc ugacagaagc     1140

aucuauaaca ccauguucaa aguggaugag aagggacucc auaauuacgu gcacuacauu     1200

aaacagggcc gcacagaaga gacguccugu aaccgggagg acuuuuauaa guacaccaaa     1260

aaggucguug aaggccugcc ugauucuaag gacaaagagu auauccugag cgaaaucgag     1320

cuccagacac ugcuuccccu gcagagaauu aaggauaaug gggugauccc uuaccaacuc     1380

caccuggagg aacugaaagu gauucuugcu aagugcggac ccaaauuccc uuuucucaac     1440

gaggucgccg acggcuucuc cguggccgaa aagcugauca aaaugcugga guuucgcauu     1500

ccguauuacg uugggccccu caacacccau cacaaugugg auaacggagg cuucgcuugg     1560

gccguccgga aggcgagcgg gagagugaca ccuuggaauu uugacgacaa gaucgaucgc     1620

gaaaaaucug cugccgccuu cauuaagaac cugaccaaua aauguacgua ucuucuggga     1680

gaggacguuc uccccaaguc cagccugcug uacucugagu uuaugcuucu caacgaacug     1740

aauaacgugc ggaucgaugg caaaccucug gagaaggcug ucaaggaaca ucucaucgag     1800

gccguguuca aacaggacca caagaaaaug acaaagaaua gaauugaaca guuucugaaa     1860

gauaacaauu acauccccga gaagcauaag ccggagauua ccggccuuga cggggaaauc     1920

aaaaacgauc uggcuuccua ucgcgacaug guccggauuc ucggaaauaa cuucgaucug     1980

agcauggccg aggaaaucau caccgacauu acaaucuucg gcgaguccaa gaaaaugcug     2040

agagaaaccc uucgcaauaa guuuucuagc cggcucgaug acgaggcgau uaaaaagcug     2100

uccaagcuga gauaccggga uugggggcgc cucucuagaa aacugcuuaa cgugaucgag     2160

ggaugcgaca aggccggcga cgggacaccu gaaacgauua ucauccugau gcggaacuuc     2220

agcuauaauc ucauggagcu gcugggcgau aaauuuuccu ucauggaacg cauucaagag     2280

aucaacgcua agcugauuga aggacagauc guuaaucccc acgacauuau cgaugaccuc     2340

gcccugagcc cugcugugaa aagagccguc uggcaggcgc ugcggaucgu ggaugagauu     2400

guucacauca agaaaacccu ccccgccaga auuuuugugg aggucacacg cucuaacaag     2460

gcugaaaaga aaaagaaaga cuccagacag aagcggcuga gcgaucuuua cgccgcuauc     2520

aaaaaggacg augcccugcu cucuggccug accaaugagg aguucggggg acugaagucc     2580

ggccuugcca aauaugacga ugcugcgcug cgcagcaaga aacucuaccu guauuacaca     2640

caaaugggga gaugugccua caccggagaa gugauugacc ucucucagcu gaacacggac     2700

aauuaugaua ucgaccauau uuacccccgg agccuuacca aggaugacuc cuuugauaac     2760

cuggugcucu gcaaacgcac agcuaaugcc caaaagagcg acaccuaucc uaucucuaag     2820

gagauccaga aagcccagaa ggcuuucugg auuuuucuga aacagcaggg ccugaucucc     2880

gaaagaaagu acgagcggcu uacacgcauu accccgcuga cggccgauga ccucagcggg     2940

uucaucgcga gacagcuggu ugaaacaaac caauccguca aagcugccac cacacuccug     3000

cggcgccuuu aucccggcgu ggaugugguc uuugugaagg ccgagaaugu uaccgacuuc     3060

agacacgaua acgacuuuau uaaggugcgg ucucugaauc aucaccacca ugcuaaagau     3120

gccuaccuca acaucgucgu gggaaauguu uaucacgaaa gauucacccg caacuuccgg     3180

gcguuuuuca agaaaaacgg cgcuaauaga acauacaacc uggccaagau guuuaauuac     3240

gacgugaacu guacgaaugc caaagacggg aaggcuuggg augucaagac cagcauggac     3300

acagugaaaa agaugauggc cuccaacgau guucgcguga ccaaacggcu gcuugagcag     3360

ucuggagcuc uggccgacgc gacagucuau aaggccgcug uggccgagaa agcuaaggau     3420

ggcgccuaca ucgggaugaa aaccaagagc uccguguucg ccgacgucuc uaaguaugga     3480

ggcaugacga aaauuaagaa ugcuuacagc aucauuguuc aguaugcggg gaaaaagggc     3540

gaagugguga aagagaucgu cccucucccc auuuaccuga caaacagaaa uaccaccgau     3600

caggaccuca ucgauuaugu ggccuccauc auuccucaag cuaaggacau cagcauuauc     3660

uaccgcaagc ugugcauuaa ccagcuuguu aaagugaaug gauuuuacua uuaccugggc     3720

gggaagacaa acucuaaauu cuguaucgau aaugccauuc aggucaucgu guccaacgaa     3780

uggauccccu aucugaaggu ucucgagaaa uuuaauaaca ugcggaagga caacaaggau     3840

cugaaagcca augugauuag caccagagcu cuugacaaca agcauacaau cgaaguccgc     3900

auuguggagg agaaaaauau cgaguucuuu gacuaccugg uuucuaagcu caaaaugccg     3960

auuuaucaaa agaugaaggg aaacaaagcc gcggaacugu ccgaaaaggg cuacggacuc     4020

uucaaaaaga ugagccugga ggaacagucu auccaccuua ucgagcugcu gaaucuccug     4080

acgaaccaga aaaccacauu ugaggugaag ccucuuggga uuaccgcuuc ccggagcaca     4140

gucggcucca aaaucagcaa ucaggaugag uucaagguga uuaacgaauc uaucaccggg     4200

cuguauagca augaagugac gauugucuga                                      4230


<210>  4468
<211>  4233
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4468
augaccaaga acauccuggg ccucgaccug ggggugagcu ccauuggaug ggcccuuguc       60

caggaaaauu cuaaaaaccc cgaaaauaac aagaucguua aacugggcgu gagagugaau      120

ccucucacag ucgaugagca aauuaacuuc gaaaagggga aaccgaucac gaccaaugcu      180

agccggacac uggcccgcuc cgcgagacgg aaccuucagc gcuuuaagcu gagacgggcu      240

aaucucauug acgugcugaa gaaaggaaac auccugaagc agggcgaucu ccugaccgag      300

aucgggaaaa auucuacauu ccagacccaa gaacuucgcg ccaagagcgc caaagagaag      360

guugagcugu ccgacuuugu gcggauucuc cugcuuauca acaagaaaag aggauacaag      420

ucuagccgca aagcuaagaa ugaagaugag ggccagauua ucgacggcau ggccgucgcu      480

aaaaagcugu augaagagaa cauuacaccc ggggaguacu ccuauaaacu ccugcaggaa      540

ggaagaaagc agcugccuga uuucuaccgg agcgaccucc aagccgaguu ugauaagauc      600

uggaauuucc agaaacaguu uaaccccgaa auuuucacca acgagcugua uaagcgccuu      660

cagggcaaaa auagaaacgc gacguggaag gaacuggaga ucccuuuuua ccucgugggg      720

aucaaacaaa uuggaaccau gcaggagaag aagaucgaac gguauuucug gcgcucugag      780

gccguuaaaa agcagcugga cuuugaaucc cuugcugugg ucuuccaaga gauuaauagc      840

aaccugaaua acucuuccgg cuaccucggg gccaucagcg auagaucuaa agaacuguac      900

uuuaauaacc agacaguggg agaguaucug uacaaucagc ucaagauuaa cccccacacc      960

aaacugaaga aucagguguu cuaucggcaa gacuaccuug augaguucga aaagaucugg     1020

gagacacagg cuaaauauca uaagaaacug accaaggaac ucaaagagga aaucagagac     1080

auugucaucu uuuaccagcg caagcugaag ucccagaaag gccuuauuag cuucugcgag     1140

uuugagaacc gggaaaucga gauuacgguu aauggcaaga caaagaagaa aaccgugggg     1200

cugaaggugg ucccuagauc cucuccccuc uuccaagaau uuaaaaucug gcagcgccug     1260

aacgaccugg aguucaagaa uguggagaca aaggaaaucu uuccgcucga gcuggaacac     1320

aaagagauuc uuuucggaaa ccugaacauc aagggcaauc ucagcuccaa agaacugauu     1380

gagcagcugg gguauccuag caaggagugg aaauguaacu uuaaggaaau cgauggaaau     1440

aagacccaug agaaccuuua caccgccuuc cucaaaauua ucgcgaauga aggcauugag     1500

uuucccaagg aguucaaacu gucuaucgac gaugacauca agguuuccaa aauugaugug     1560

agcucugaga agaucaagga guuuauuaaa gaaaacuucc ugaagcucgg gaucaauaca     1620

agcauucugg acuucaaccc ugagcuugau ggaaaagacu uugaaaagca ggaguccuac     1680

caacuguggc accuccugua uucuuacgaa ggcgaugaca gcgccuccgg caaugagaaa     1740

cuguaugagc uucucgaaaa gaaguucggg uuuaaacggg aguacagcaa gauccuggcu     1800

gaugucggau ucucucagga cuauggcucc cugagcucua aagccaugag aaagaucuuu     1860

acguacauua aagaaaacaa guauuccgag gcuugccauc ucgccgggua caaucacagc     1920

aaaaacuccc ugaccaagga gcagcuugcc aaucgcaucc ugaaggaaca gcucgagauu     1980

cugcccaaaa acgcucugcg gaauccggug guugaaaaga uccuuaacca aaugauuaac     2040

cucgugaaug aggucaucaa agaauacgga aagccugacg agaucagaau ugagcuggcg     2100

cgcgaacuga aaaagaacgc cgaggaacgg gcuaauauga caucugauau cagcaaggcc     2160

acccuccugc accagaaaua ugccgaggug cuucagaagg aguucggcau uaaagcuccc     2220

uccagaaacg acaucauucg cuacaagcug uaucucgaac uggccaauaa cggguacaaa     2280

gaucuguaua caaaucaaaa gaucgagaag gaaaacaucu uuaccgacaa auacgauauu     2340

gaccauauca uuccucaguc ucgguucuuu gaugacagcu ucuccaauaa gguccuugug     2400

cccagaugug cgaaccagga uaaagacaau uauacggcug uugauuacau cgagaagaaa     2460

uacggcgaac ggggaaagga ggacuuucuc aaccgcauuc accugguguu caagagcaaa     2520

gaagccaucg ccggcaaguc ugagucccag auuagcucua ugaauacaaa auuucugaag     2580

cuccagaaaa agggguccga gaucggagaa ggcuucaucg agagagaucu gcgggacacc     2640

caauauauug cuaagaaagc caaggaaauc cuuuucggga uuaccaacag cgucaucucu     2700

acaagcggaa gaauuaccga caaacugcgc gaggauugga aucucgugaa cacaaugaag     2760

gagcugaacc uggaaaaaua ccggaaacuu ggccugacgg agcucaucgc uaauuccaaa     2820

ggggaagaga aggaaagaau cauugacugg accaagcgca acgaucauag acaccacgcc     2880

auggacgcgc ugacaguugc cuuuaccaca cauaaucaca uccaguaucu caacuaccug     2940

aaugcucgga aagaugagaa gcauaaagag caccauaaca uuuucgccau cgaaaauauu     3000

aucaccgaga ucguggaaaa gaaaaacggc agcaaggagc gcagauuuaa acuucccauu     3060

aaaaauuucc ggacggaagc uaagaaacag cuggaggagg uccucaucuc ucacaaggcc     3120

aaaaacaagg uggugaccaa gaauauuaac aaaacaaaga aaaagggagg cguugucucc     3180

aaaaucgugc ugaccccucg cgggcaacug cacaaggaaa caauuuaugg aagcgcgaag     3240

uuucuuaaaa ccaaggagga gaaaaucucc ggcaaguucg accuggaaac gauuaauaaa     3300

gugcagaacg aaaaguuuaa aaaugcccuc cuggauagac ucaaggaguu caacggggac     3360

ucuaagaagg cuuuuacagg aaagaauguc ccggccaaaa accccaucua ccugaacgag     3420

cagaagaccg aacaggugcc ugagagcguu gugcuugcuu gguaugaaac aggcuacacc     3480

auccggaaag ccgucaaugc cgauaacuuc aaggacuaca agaaucugga gaaaauuauc     3540

gauaaggggg ugaaagagau ucucaagaac cgccugaaug cuuuuaacgg caaugcgaaa     3600

gaagccuucu ccgaccugga gaagaagccc aucuggcuua accaaucuaa aggaauugcu     3660

guuaagaccg ugacaaucac gggcaucaau aacgccgaaa cccugcauua uaaaaaggau     3720

cacuucggga augagcuccu ggacgaaaac ggcaaaaaga uuccugucga cuuugugagc     3780

acaggaaaua accaucacgu ugccaucuac gaggaugcug acgggaaucu ccaggagaga     3840

guggucuccu ucuaugaagc cguggagcgg gugaaccagg gacugucuau uaucgauaag     3900

gaauacaacc aagaccuugg cuggaaauuu cuguucacca ugaagcagaa ugagauguuu     3960

cuguucccca gcgaagauuu uaacccgaaa gagacagacc ucuucgauga gaagaaucug     4020

aaccuuauuu ccaaaaauau guuucgcguc cagaaguucg ggagccugcu cucuggcuuu     4080

ugguucagac accaucugga aaccacgguu gaccucaacu ccagccugaa agauacaucu     4140

uauaagguga uccaguccug caauaaccuu aagcugaucg ugaaaguccg gcugaaucac     4200

cucggaaaga uugugcaaau cggcgaguac uga                                  4233


<210>  4469
<211>  4236
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4469
auggugaaga aagaguacuu caucggccug gacaugggga ccagcuccgu cggaugggcc       60

guugcugaua caaacuauaa ucuccugaga gccaagggca aagacaugug gggagugcgg      120

cuuuuucccg aagcgucuac ggcugccgau cgcagaagcc accggaccgc ccgcagaaac      180

aauaagcgga aagcugcccg cgagggguuc cugagagaaa uuuuugagga cgaaaucaac      240

aagauugauc cuggcuucuu ugagcggcuc aaggagucca aauacuaucu ggaagacaaa      300

cuuacagaua ccccguucgu gcuguuugcu gacaaagggu acacagauaa ggaguauuac      360

gacgaauauc ccaccgucuu ccaucucauc aaagagcuga uuucugagaa gggacuggaa      420

ccucacgaua uccgccucgu guaccuggcc ugccuuaaua ucuacaagca uagaggccac      480

uuucugaacg acacacucag cggcgauggg auugacaaua ucaacucccu guguaaugac      540

uucaccucuu aucuugcgga uuacaaccag aauagcgagg acgauggaac ggaaaagaug      600

gaccuggauu ccaccguucu cgccggcgug uuugaggaaa cacugugcaa caagcaucug      660

agcaauucug cuaaaaagcg ggaccucauu gauaaacugg ggaucaccaa caagacaaaa      720

uaucuuaccg agaugauggc ccugauuucc ggacucgcua gcucugucuc caauauguuc      780

gccaaggaca cguacgauga ggaacaaaag aaauuuaagc ugagcuucag aucuuccaac      840

cuugacgagg auauggugaa aguucaggcg augcugacac ccgaagagua ugaaaugauc      900

uguauuauga agaaaaucca cgacuggagc guggucuccg ugaucauggg cucucacaag      960

uacauuagcg acgcccgcgu ggagcucuau gagaagcaua aacaggaucu ggcuaagcug     1020

aaagccaucu acaaggaaua ccucccugac aaauaugaug cuauguuccg ggagaugacc     1080

gacaacuccu acagcgccua ugucgggaag guuucuucca auaagaaaag ccagacagga     1140

aaccacaagg ugcgcaugca aagacggggc gccaaaugca agcaggaaga uuuuuacgac     1200

agaauuaaaa aggagaucaa uucugugccc caggaagaga agauugauau ccugcaggac     1260

auggagacca acagcuuccu uccuaaacaa cugaccaaug aaaacggcgu caucccguau     1320

cagcuccauc uggcugagcu uaagaaaauu cuggauaaug cguccacaua ccuccccuuu     1380

cugaacucug ccgacgaaac ggggaucagc aauaaggaua aaauuaucca gcuguucgag     1440

uuucgcauuc cuuauuacgu gggaccccuc uucaaguccg agaaaaacaa gaccaaugcu     1500

uggguugugc ggaaggaaag cggcaaaguc uacccuugga acuuuaauga gaagaucgac     1560

gaaaaacagu cugccgagau cuucauugaa agaauggugc gccacuguac auaucugaac     1620

aaugaguccg cccuucccaa gaacagccug aaauacgagc gguuuagagu ucucaacgaa     1680

cugaauaacc ugcgcaucaa uggggagcgg auuucuccgg aacuuaagca aagaaucuau     1740

aacgaacucu ucaaggugaa aaagaaacgc auuaccaagu ccaugcugug cgaauaccug     1800

uauaaaaaug gaaucgcuga uaaggacagc gagcucuccg gcuuugacgg gacauucgcc     1860

aacgcgcugc uuucuuacgg aaaguuucgg gagcuguuca augucgauac ccucacggac     1920

aaacaggaag cugccgccga ggauauuauc aaguggagca caguguaugg cgacuccaaa     1980

aaguuccugc aggagaaaau cgaggaaaac uacggcccug agucugagaa uccccugcuu     2040

gauaacaagc aaauuaagag agucaugggg uacaaauuua ccgacugggg aaaucucagc     2100

cgcgaguucc ugaaccugga aggcgcugau aaggacaaug auaccacaac cggggaagug     2160

augccucuca ucacacggau gugggaggag aacuauaauc ugauggaacu uauguccaac     2220

aaauuuagcu acagagacgc ccuggcugag aagacggaag agauugagaa aacccucaca     2280

gauaucgaau augaggaccu ggaagaucug uacauuucug cccccguuaa gcggaugacc     2340

uggcagacac uucucauccu gaaagagauc uaucagauua ugggauacgc gcccaagaag     2400

aucuucgugg aaauggcccg cgaggaagag gagaaaguca acggcaaggg ggcuagaaaa     2460

uccagccgga agaaaagacu ggaagaccuc uaucgcaauu guaaggacgc cucuaaggag     2520

cuguccaccg gcauuaaaaa cgcugaugaa gccauccuuc ggagaaagaa acuguaccuc     2580

uacuaucugc agaagggaaa augcauguac agcggcaaga cgauugacuu uaagucucug     2640

uucaccaaua acuaugauau cgaccacauc uacccucaaa gcgugguuaa agaugacucc     2700

cuugagaaua acauggugcu cgucgaacgc aauuauaaca auaagaaaag cgauacauuu     2760

ccgauuuucu cugacuggca gaacaagaug aaacccuuuu ggaccaugcu gaaggagcag     2820

ggguucauca augaggaaaa guacaaaaga cugacacgga ccgaugagcu cacggacgau     2880

gaacuggcga acuuugugaa ucgccagcuu guggagacaa gacaagccac caagguuauu     2940

gcugagcugc ucaaaaacuc cuucgacgac aucgauagca agauugucua ugccaaagcu     3000

ggaaaugugu ccgaauuucg gcauaaguuc ucuaugaagu acgacaaaga gacagaaaag     3060

agcuccguga uccacccuga gcuggucaaa ugucgcauca uuaacgauuu ccaucacgcc     3120

aacgacucuu accugaauau cgugguuggc aacauuuaug augugaaguu uacccagaau     3180

cccuacaacu auaucaaaga auacaagaaa cagaagaaua acagcgcccu uuccgagcag     3240

gagaaggaaa aauaucauau ggacaagauu uucaauuuug augucaaaag aaacggggag     3300

guggcuugga ucucucgggg agacaauaag acccuggaaa caguucucaa agugaugcgc     3360

aagaacacgc cuauugucac cagaaugaau uucgaggaac acggccaacu ggcggaucuc     3420

acaaucuaca gcgccuccga cgcucugagc aagaaagggg ugggcuauau caagguuaaa     3480

gccucugaug cccggcuuga cgugcugaag uacggaggcu acaaaaagua uaagggggcu     3540

uacuuuuucc ucgucgagca caccgagaaa aacaagcgcg ugagaacaau ugaaaauaug     3600

ccccuguauc ugaaaaacga gcuuaauacc aaggagaaaa ucgaacugug gugcgccgau     3660

aaggaaaacg gacucggccu gguggacccg gacguccggc ucgggaagau uaaacuguac     3720

uccaagauca gaauugaugg auuugaccuu uaucugacgg gccgcacagg gaauaaccuc     3780

cugaccagca acgcgguuca gcugaaaauc ggcucuaaua agccuuccga uaacgacaaa     3840

cuuuucuggc gguacuauau caauaagcug agcaaauaca ccgagaacuc ugaguuugug     3900

uccauucaga auaacaucaa gcucuacgau cuguucgugg aaauuaauaa cgcuggaguc     3960

uauagcaaga gacagaaucc caucggcgac aaacuccugg aggggcgcuc caaguuuauu     4020

aaccuuacac ugagcaaaca aguggaaacc cugcucggaa ucauccggau uuacucucug     4080

gagaaucagg gcgccgaucu uagcaacauc gggggacagg agaagaaugg cacaaugaaa     4140

ccucauaaga gaauuuccga caagaaagaa guucugcuca ucaaccaauc ugugacgggg     4200

cuguaugaga gcaagauuga ucuccugaaa aucuga                               4236


<210>  4470
<211>  4236
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4470
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugggc ccuuaucaaa       60

cagaacgcug aggaaagaca aggcuccauu cuggggaugg gaacacgggu gcuccccaug      120

ucucaggaug uccugggcga cuucgggaag ggaaauagcg uuucccagac ggccgcgcgc      180

accggcuaca gaggcauccg gcgccuuaga gagcgguuuc ugcuccgcag agaacggcug      240

caccgcgugc ugaacauucu cggguuccug ccuuauuacu augcugccca gaucgauuuu      300

gacaaaagau ucggaaaguu uaaagccgag acagaaccga aacuucccua caauaacgag      360

ggcuucaucu uuaaaaauuc uuucgaggaa augcugagcg auuuuaaaau uucccaaccu      420

gagcuccugu cugggaacaa gaaagugccc uaugacugga ccaucuacua ucuucggaag      480

aaagcuguca gccagaagau ugaaaaggag gagcuggccu ggauccuccu gaauuucaac      540

cagaaacgcg gauacuacca gcugagaggc gaagauuuug acgaggaaaa ggauaaaaca      600

uucgugaccc ucaauguuga caaggugauu gauuccgggg agaaagucaa gggaaacauc      660

cuguuugaca uuuauuucac aaauggcugg aaauacgaaa agcaaaccac gaagccugag      720

gauuggcuug gccggagcaa agaguucauc aucaccgaau cucugcucaa gaacggggag      780

gugcgcagaa cauuuaaagc uguugacucc gaaaaggacu ggauugccau caaagcgaag      840

accgagcagg aaauugagac agcccauaag accgugggaa gcuauaucua cgaugcuauu      900

cugcagaauc ccaaacagaa gaucaaaggc aaacuugucc ggacgaucga gagaaaguuc      960

uauaaggagg agcugcgcaa gauucucgaa aaacaaaagg acuuucaccu ggagcugcag     1020

aacgaagauc ucuacucuga cugcgugcgg gagcuguaua gaaacaauga uacacaucag     1080

cuuucccugg ccaaaaagag cuucauccac cucuuucugg aggacauuau cuucuaccaa     1140

cgcccucuua aaucucagaa guccagcgug uccaacugua cccuggaaug ccggaaguac     1200

agagauaaaa auggggcuga gggcauucag uuucucaagg ucaucccgaa aucuaacccc     1260

uauuaccagg aguuccgcau cuggcaaugg augagcaauc ugaagauuua ucggagagac     1320

gaugacguua acaucacauc cgaaugucug aaaaccgccg aggauuacga agaccucuuu     1380

guguuccuga auauucgcaa ggagguggac cagaaagcgu gccuucggca uuuuaagcug     1440

agcgaaaaga cccacagaug gaacuauguc gaggauaaaa aguacccuug uaaugaaaca     1500

ggaacgauga ucucugagcg ccucaaaaag gugggggacu cccccgauaa cauuuucacc     1560

cgggagaugg aacagcagau cuggcacauu aucuauagcg uuaaugacaa aacagaguac     1620

gaaaaggccc ugaagucuuu ugcuaaaaag aacgagcugg auaaugacag cuucuucgaa     1680

gccuuuagaa aauucccucc cuuugauucc gaguacggau cuuucagcga gaaggcuauc     1740

aaaaagcucc uuccgcugau gcggauuggc aagcauugga acugggaagc cguggacgag     1800

aaaaucaaga aucgcauuca aaagaucauu aucggcgaau augaugagaa caucaaggac     1860

aacauuagag aaaagacccu gucccuccag aaggagagcg auuuucaggg gcugccucuu     1920

uggcuggccc aguacgucgu guauggacgg cacucugagg cuuccguugc gggcaagugg     1980

aauagcgugg acgauaucga agaguaccuc aaagaguuca agcaacauuc ucugagaaac     2040

cccauugucg aacaggugau cauggaaaca guccgcgugg uuaaagacau uuggaucaag     2100

uaugggaaug gagccgcuga cuuuuucgau gaaauccaca uugagcuggg ccgggaaaug     2160

aaacuuccug ccaaggagag aaacaaaaug acccagaaga ucuccgaaaa ugagaacacg     2220

aaucuccgca uuaaggcccu gcuggcugag augaugaacg acagcgaagu gcagaauguc     2280

agacccuacu cccccaugca acaggagauc cucaaaauuu augaagaugg ggcccugaac     2340

ucugagauca gcguggacga ugacauccuu aagauuucca aaacagcgca gccuucuccg     2400

agcgaucuga agaaauacaa gcucuggcug gaacaaaagu accggucccc cuauaccggc     2460

gacaucauuc cucugaauaa acuuuuuacc acagaguacg agaucgaaca cauuaucccc     2520

cagagccgcu auuucgauga cucuuuuucc aacaagguua uuugcgagag ccucgugaau     2580

agacugaaag auaacuacau cggacugggc uucaucaagc aguuucaugg guauaaaauu     2640

ucugacaaga ccaaggucuu cgaaguggag gaauacgagg auuucgugaa aaagaauuau     2700

gcuaacaacc gguccaaacg cagcaagcuc cugcuugagg aaaucccuga ggaaaugauu     2760

gagagacagc ugaaugacac acgguacauc ucuaaauaca uuagcggaau ccucuccaac     2820

aucguucgca agaaggagaa ugacgaaggc gucaacagca aagauauuau ccccgggaau     2880

ggaaagauua cgucucaacu gaaacaggac uggggccuga acgaugugug gaaugaccuu     2940

auccucccga gauuugagcg gaugaacgaa cugaccaagu ccagcuauuu cacagugugg     3000

aaugagaaau accagaaguu ucugccuacc guccccgugg aguucuccaa gggguuuucu     3060

aaaaagcgca uugaucacag acaucacgcc cucgacgccc ugguuaucgc uugugccaca     3120

aaagaucaug ugaaccuucu gaauaacaag agcgcuaaau ccgaaaugaa gcgguaugac     3180

cucaaaagaa agcugaugaa guucgagaaa aagaccuacc agcacucuca aacgggcgaa     3240

cgcaucguca aagaugugcc uguugaguuu cugaagcccu ggaaugacuu caccguggaa     3300

gccaaagaga accuugaaaa gauuaucguc agcuuuaagc agaaucugcg ggugauuaac     3360

aaagcgacaa accacuauga gaaguggauc gagaaagaua auguuaaggu gaaaggacuc     3420

gucgaacagc agggccugaa cugggccauu agaaagccuc uccauaagga caccgugucc     3480

gggaaagugg accugcccgg aaucaagguc ccgaaaggca agauucuuac agcuacccgc     3540

aaaucuaucg auacgagcuu cgaccugaag acaaucaauu ccauuaccga uacagggguu     3600

caaaagaucc ucaaaaacua ccuggaguau aagggaaaua gcucugaaga ggccuuuucc     3660

agcgaaggca uugaggacau gaacaauaac aucgagaaau ucaaugaagg gaagcugcac     3720

cagccuauua ccaaagugcg gaucuucgag cuuggcucua aguuuccccu gggacagauc     3780

ggcaacaaaa agcauaagua cguggaaacc gcuaaaggga caaaucucuu cuuugccauu     3840

uacgaggaug acaagggaaa aagaacguau gagaccaucc cccugaacga agucauugag     3900

cgccaaaagc agggccucgc gccugugaca cugaaaucca agaaugauuu cuaccuuugc     3960

ccgaacgacc ugguuuaugu ggccgcugaa ggggagagcg ucaauaagau cgauuuuaaa     4020

uacgccucuc aggaauuuau ucacaacauc uauaaggugg uuuccuucag cggaucccag     4080

aucuucuuug ugaaacaaaa uauugcuguc agcaucguga acaaggccga guucucuacc     4140

cucaacaaaa uggagcgggc cguugacggc aagaugauua aggauuguug caucaaacug     4200

aagauugaca gaaucgggaa uauuacaaaa agcuga                               4236


<210>  4471
<211>  4239
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4471
augaugacca agaacauccu gggccucgac cuggggacaa auagcauugg augggcccuu       60

aucaaacaga acgcugagga aagaaagggc uccauucugg ggaugggaac gcgggugcuc      120

cccaugucuc aagaugcccu gggcgacuuc gggaaaggaa auagcgucuc ccagaccgcg      180

gcucgcacag gcuacagagg cguucggcgc cuuagagagc gguuucugcu ccgcagagaa      240

cggcugcacc gcgugcugaa cauccucggg uuccugccug auuauuacgc cgcccagauu      300

gacuuugaua agagauucgg aaaauuuaag gcugagaccg aaccgaaacu ucccuauaau      360

aacgagggcu ucaucuuuaa aaauucuuuc gaggaaaugc ugagcgacuu uaagaucucc      420

cagccugagc uccugucuga aaacaaaaag auucccuacg auuggacaau cuauuaccuu      480

cggaaaaagg ccgugagcca acgcauugag aaggaggaac uggucuggau ccuccugaau      540

uucaaccaga aaagagggua uuaccagcug cggggagagg acuuugauga agagaaggac      600

aaaaccuucg ugacacucaa uguugauaag gugauugacu ccggcgaaaa agucaagggg      660

aucgugcugu uugauguuua cuucaccaac ggauggaaau augagaagca gauuacgaag      720

ccugaggacu ggcuuggccg cagcaaagaa uuuaucauca ccgagucucu gcucaagaau      780

ggcgaaguga aaagaacauu uaaggcuguc gacuccgaga aagauuggau ugccaucaag      840

gcgaagaccg aacaagagau ugagaucgcc cauaaaacag uggggagcua cauuuaugac      900

gcuauccugc agaaccccaa gcagaaaauc aagggaaaac uuauucggac caucgaaaga      960

aaguucuaca aggaggaacu gcgcaaaauu cucgagaagc agaaagaauu ucacccugag     1020

cugcaaaaug aggaucugua uucugacugc gugcgggaac ucuacagaaa caacgaugcc     1080

caucagcugu cccuugcuaa gaaagacuuc auccaccugu uugucgagga uauuaucuuc     1140

uaucagcgcc cgcucaagag ccaaaagucu uccguuagca auuguacgcu ggaaugccgg     1200

aaauacaagg acaaaaacgg cgaggagaga auccaguuuc uuaaggugau ucccaaaucc     1260

aauccuuacu aucaggaguu ccgcaucugg caguggaugu cuaaccugaa gauuuaccgg     1320

agagaugacg augugaauau cacaagcgag uaucucaaaa ccacagaaga cuaccaagac     1380

cuguuugucu uccugaacac ccgcaaggag guggaucaga aggccugucu caaacauuuu     1440

aagcugaaug aaaagaccca ccgguggaac uauguugagg acaagagaua ccccugcaau     1500

gagacaggga cgaugauucu ugaacgcuuc aaaaaggugg gagauuccgc ggacaacauc     1560

uuuacccggg agauggaaca gcagaugugg cacauuaucu auagcgucaa ugauaagaca     1620

gaguacgaaa aagcccugaa gucuuucgcu aaaaagaacg accucgauaa uguguccuuc     1680

uuugaggccu ucagaaaauu uccucccuuc gacagcgagu acggcucuuu uagcgaaaag     1740

gcuaucaaga aacugcugcc gcuuauucgg accgggaagu auuggaacug ggaggccguu     1800

gaugaaaaga caaagaaacg cauccaaaag augauuaccg gagaguggga cgaagauauc     1860

aaggacaaua uuagagagaa aacgcucucc cugcagaagg agucugauuu ccagggccug     1920

ccucucuggc uggcccagua cgcuguguau ggccggcaua gcgaagcguc cgucgccggg     1980

aaauggaaca gcgugaacga cauggaggaa uaccuuaagg aguuuaaaca acacucucug     2040

agaaauccca ucgucgaaca ggugaucaca gaaaccauuc gcguugugaa ggacaucugg     2100

auuaaauaug gaaacggcgc ugccgauuuc uuuaaugaga uccauauuga acucgggcgg     2160

gagaugaagc ugccugccaa ggaaagaucc aaaaugaccc agaagaucag cgagaacgag     2220

aauacaaacc ugcgcaucaa agcccuucuc gucgaaauga ugaaugacuc ugaggugcac     2280

aacguuagac ccuacucccc caugcagcaa gaaauucuga agaucuauga ggauggagcu     2340

cugaauagcg aaauuuccgu ggacgaugac auccucaaaa uuucuaagac cgcgcagccu     2400

agcccguccg aucugaagaa auacaaacuu uggcuggagc agaaauaccg gucucccuau     2460

acaggcgaca ucaucccucu caacaagcug uucacgaccg aguacgaaau ugagcacauc     2520

auuccccaaa gccgcuauuu ugaugacucc uucagcaaua aagucaucug ugaaucuucc     2580

gugaacagac ugaaggauaa uuacauuggg cuuggcuuua ucaagcaguu ccauggauau     2640

aaaauuagcg acaagacaaa aguguucgag guugaagagu acgaggauuu ugucaagaaa     2700

aacuaugcuu cuaauugguc caagcggagc aagcuccugc uggaagagau cccugaagag     2760

augaucgagc gccagcucaa cgacaccaga uacauuucua aauacaucag cggcauucug     2820

uccaacaucg ugcggaagaa agaaaaugac gaugggguga acagcaagga cauuaucccc     2880

ggaaauggca aaaucacauc ucagcuuaag caagauuggg ggcugaacga cgucuggaau     2940

gaucucgugc ugccgcgcuu cgagagaaug aacgaacuga ccaaguccaa ugacuuuacg     3000

guuuggaacg agaaauauca gaaguuccuu ccuaccgugc ccgucgaauu uagcaaagga     3060

uucuccaaga aacggauuga ucaccgccau cacgcccucg acgcccuggu gaucgcuugc     3120

gccacaagag aucauguuaa ucugcucaac aaucagucug cucggagcga gaugaagaga     3180

uacgaccuga aacgcaaacu uaugaaguuu gagaagaagg ccuaucagca cucccaaacc     3240

ggcgaacgga uugugaaaga ugucccugug gaguuccuga agcccuggga caacuuuauc     3300

guugaagcga aagagaaucu cgacagaauu aucgugucuu ucaagcagaa ccugcgcguc     3360

aucaauaagg ccacaaacca cuacgaaaaa uggauugaga agaacaaugu gaaagugaag     3420

gcucuggucg agcagcaggg gcuuaacugg gccauccgga aaccucugca uaaggauacc     3480

guuagcggca agguggaccu ccccggaacg aaagugccga agggcaaaau ucugacagcu     3540

accagaaagu ccaucgauac aucuuuugac augaaaacca uuaauagcau caccgauaca     3600

gggauucaaa agauccucaa gaacuaucug gaauacaaag gauccuauga ggacgccuuc     3660

agcccugaag gcaucgagga uaugaauaag aacauugaaa aauucaauga cgggaagcag     3720

caccagccca ucauuaaagu ccgcaucuuu gagcuuggau cuaaguuccc ccugggccaa     3780

auugggaaca aaaagcauaa guacguggag acggcgaaag gcaccaaucu cuuuuucgcc     3840

aucuacgaag augacaaggg aaaacggaca uaugagacca ucccucugaa cgaaguuauu     3900

gagagacaga agcagggccu ggcuccggug acauuuaaau ccaagaauga uuucuaccuu     3960

agccccaacg accuggucua uguggccgcu gagggggaau cuguugauaa ucucgacuuu     4020

caguccgaca gccaagagua caucaacaau guguauaagg ucgugucuuu cuccggaagc     4080

cagauuuucu ucguuaaaca gaacguggcc gucuccaucg ugaauaaggc cgaauuuagc     4140

acccugaaca aaauggagcg cgcuauugau ggccggauga ucaaggaaug uugcgugaaa     4200

cucaagaucg acagaauugg gaacaugacg aagucuuga                            4239


<210>  4472
<211>  4245
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4472
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cuucguggag       60

gaagcugaga augaauccga gaagucuagc aucauuaaac uuggcgucag aguuaacccc      120

cugacagugg augaacagca aaauuuugag aaggggaaau ccaucacgac caacgccgac      180

cggacacuca agcgcucuau gagacggaau cugcagcgcu acaagcagag acgggagaac      240

cuuauugaua uccugaugaa aaauggauuc accagcgacg auacaauccu cuccgaaaac      300

ggcaaucgca ccacauuuga gaccuauaga cugcggucua aggcgguggc ugaaaagauu      360

agccugcagg aguucgcccg cguccuccug augaucaaca agaaaagagg guacaagucc      420

agccggaagg ccaaaggaac ggaggacggc gaacuuauug auggcaugga gguggcuaag      480

aaacuguaua auuacaaccu caccccuggg caacugugcc uucagcugcu caaugaagga      540

aagaaacauc ugccggacuu uuaucgcucu gaucugcagg ccgaguucga cagaaucuuu      600

gauauucaga ugacauucua caccgaauau cucacacugg agcuuaagga ggaacugcgg      660

ggcaaaaagc gcgacgcugu uugggccauc ugugcgaaga uuuuuaaaga gaagggguuc      720

gaacucaucg gaaccaaaag acaaggcucc acggccgagc agaagcacga aaacuacagc      780

uggcgggcua aagggcuguc ugagaaacuu gagcuggaac agcucguggu cgugcugcag      840

caaaucaaua uucagcugaa cuccagcucu ggauaccucg gcgccaucuc cgauagaagc      900

aaggagcugu uuuucaacaa acagacaguu ggccaauauc agauggcugu gcuugacaag      960

aaucccaacg ccgggcugcg caauaugguc uuuuaccggc aggacuaucu cgaugaguuc     1020

aacgcgauuu gggaaaaaca ggcccuguuc cacccuaucc uuaccgacac acugaaggau     1080

gaaauuagag acgugaucau cuuuuaccaa cgccggcuca aaucccagaa gggacugauu     1140

ucuuucugcg agcuggagag cagaaagauc gaaguggaga uugcuggcca gaauaaaacc     1200

aagauggucg ggucccgcgu uaucccccgg agcucuccuc ucuuucagga guucaaaauu     1260

uggcaauccc ugaacaauau cgaaguguuu ggaaagggca aaaagagcaa gaaacagaag     1320

gagcagaccc uuuucgauca gacagaagac ccccuggagu uugggggaag acgcgaacuc     1380

uaugaggaag agaaagagcu gcuggcccuu gaacucucua ucaagaaaga gcugaagaaa     1440

gcugauauuc ugaagcuccu guucgacaac ccuaaggaac uugaucugaa uuuuaacgcc     1500

gugcaaggca aucucacgca ggccacccug uucaacgucu acaaaaagau cauugagcug     1560

agcggccaug aaaucgacgu ggagaaagau uccgcuucua agauucuuaa aagcaucgag     1620

gaaauuuuua aggcgcucaa uuauaacaca gagauccugc acuucgacuc cgaaaagagc     1680

caggaccagc aagccaugua caugcugugg caucuccugu acucuuuuga gggggauaau     1740

uccaacaccg gaaacgaaaa ucuuaucaac aaaauuagca agcuguaugg cuucgagaaa     1800

gaguacgcua agauccucgc caauguuucu uuccaggacg auuauggguc ccugagcgcc     1860

aaagcuauuu ccaagauccu gccguaccuu aaggaaggac acaaaucuga cauugccugu     1920

gaucucgcgg gcuauaagca cagcgcuucc ucucugacac gggaggaacu ggagaacaaa     1980

accuacaagg acagacucga gauccugccc aaaaauacgc uucgcaaccc uguggucgaa     2040

aagauccuga aucagaugau uaacgugauc aaucucauua ucgauagccg ggagcugaag     2100

aaauccgcca aggaaagaga ggaacugaca aaaagcauuu cugagaccac caaacuucau     2160

caacaguaua aagacauccu cgccaaggcu cccuuuaacc ugacaaauac cucccggaac     2220

gauaucauuc gcuacaaacu guacgaggaa cucaaggaca auggguauaa gacacuguac     2280

agcaacaaau auaucucucc ugccgcucuu uucuccggcg auuuugacau ugagcacauc     2340

auuccccaga gcagacuguu cgaugacucu uuuagcaaua agacgcucga acugcggucc     2400

aucaacaucg agaaaggaaa caagaccgcc uacgacuuca uuaaaggcaa guauggggaa     2460

acaggagagg auaauugccu ggacaaguac cuuaacagaa ucgagacccu guauaaaaau     2520

ggcauucuca agaaaagcaa guacaacaaa cugaagaugc aggauaagga caucccggau     2580

ggguuuauug aacgcgacau ccgggauaca caauacauug cgaaauaugc caagacccuc     2640

cugggagacc uuguuaaauu cguggucucu acguccggca gcgugaccga gagacugcgc     2700

gaagauuggc agcucgucga cgugaugaag gagcugaauu gggaaaaaua caugcagcug     2760

gggcuuacag aggaguuuac caacgaagag ggccauagaa uccggaagau caaggauugg     2820

acaaaacgca augaccacag acaccaugcu auggaugccc ugaccguugc uuucacgaag     2880

cggcaguuua uucaauaucu caacaaucug aacgcccgca gaaucuccga cgaacaggga     2940

auuucuaaua gcgagaaaga cgagcggaac aaugugucca ucacaaccaa cgauaugaag     3000

cucaauacac gcgacguccu gggcauugaa aagaaggagc uuuacagaga uaaccggggg     3060

aagcugcgcu ucaaagcgcc uaugccccuc cuggaauuua gagaggaagc caagcggcac     3120

cuggagaaca cccuuaucuc uaucaaagcu aagaauaaag ugaccacaau uaacaucaau     3180

acgcugaaga aagccggagg cguuuauaag aagacccagc ucacaccuag agggcagcug     3240

cauaacgaga ccauuuacgg aagccaaaaa caguauaucg ugaaggaaga gacaauuaau     3300

gcuaaauucg acgucgaaaa gaucgccacc gugacgaacc ccguguaccg cgaggcccuc     3360

cugaaacggc uugaacagaa uaacaaugau cccaagaagg cuuucacagg cuccaacgcg     3420

cugaccaaaa agccuaugug guuugacgag caacagaccg agaaaguucc ggaaaagguc     3480

aaaacagugu cuuucgagac caucuacaca auuagaaagg aaaucagccc cgaucugaag     3540

auugagaaag uggucgacga aaaggugcgc cagauccuca auaaccggcu gaaugaguau     3600

aacgggaaug ccaaacaggc uuuuuccaac cuugaugaga auccuauuug gcugaacaag     3660

gaaaaaggca ucacgauuaa gagaguuacc aucacaggaa ucaacaaugc ccaagcccuc     3720

cacgagaagc uggacaaaga uggcaagccc auucucgacg gggaaggcaa aaagcagccu     3780

guggacuucg ucaacaccgg aaauaaccau cacguggcug uuuacuguga ugccgacggg     3840

aaucugcagg agaacgugau cagcuuuuau gaggcgguca cacgccggaa ucuuggacug     3900

cccauuaucg auaaagacua caagaaagau gacggcuggc aguuccuguu uucuaugaag     3960

caaaacgaau auuucguguu uccgaaugag uccaccgggu ucaacccuaa ugaaguugau     4020

cuccugaacc ccgacaauua cagccuuauu ucuccuaacc uguuuagagu gcagaaguuc     4080

acgaucaaag auuauuucuu ucgccaccau cucgagacca augucgaauc caacagcaca     4140

cugaagggca ucaccuggcu ccggugcgga cugaacaaua uugacaacau cauuaaagug     4200

agaaucaauc acauuggcca gaucauccau gugggggagu acuga                     4245


<210>  4473
<211>  4248
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4473
auggccaagu ucaacaaacc cuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguua ucaccgagga uuacagagug ccugccuuuc gguucaaggu gcucggcaau      120

acagaaaaag agaagauuaa aaagaaccug aucgggagca cgaccuuugu cccggcgcag      180

uccgcucaag ugacacgcgu uuucagagug aaucggcgca gaauugaccg gcgcaaccac      240

agaaucgccu aucuucggga uaucuuucag aaggaaauug agaaagucga caagaauuuc      300

uaccgcagac uggaugaauc uuuucgggug cucggagaca aaagcgagga ucugcagauc      360

aagcagcccu ucuuuggcga caaagagcuu gaaaccgccu aucauaagaa guacccuaca      420

auuuaucauc ugcgcaaaca ccucgcugau gccgacaaga acucccccgu ugcugauauc      480

agagaggugu acauggcccu gucucacauu cugaaauacc gggggcauuu ccucacccug      540

gacaagauca auccuaacaa uauuaacaug caaaauagcu ggaucaacuu uaucgaaucc      600

ugccaggagg ucuucgaucu ugaggugagc gaagagucua aaacaauugc ggccaucuuu      660

aaguccagcg aaaaucgcca ggagaaagug aagcagauuc ugucuuauuu ccaacaggaa      720

cuccugaaga aagacaaguc caucuucaaa cagcuucugc agcuccuguu uggacugaag      780

accaaauuca aggacuguuu ugagcucgag gaggaacccg aucugaacuu cagcaaggaa      840

aauuacgacg agaaccuuga aaaccugcuc ggcacgcugg aggaggauuu uucugacguc      900

uucgcuaaac uuaagauucu gagagauacc auccuccugu ccgacaugcu gacauauacc      960

ggcgccacac acgcucgguu uagcgccacc augguugaaa gauacgagga acaccgcaaa     1020

gaucuccaac gguucaaguc cuuugugaaa cagaaucugu cugagcagga cuaucuugau     1080

aucuucggga gaaagacgcc uaacggauuu gacguggaua aggaaacaaa aggcuacguc     1140

ggguauauua gcaauaagau ggugcugacc aacaaacaaa agacaaucca gcagaauuuc     1200

uacgacuaca uuuccggaaa aaucaccggc auugaggggg cggaguauuu ucucaacaag     1260

aucagcgacg gaaccuuccu gcgcaagcug cggacaacgg auaauggcgc caucccgaac     1320

cagauucaug cuuacgaacu ggagaaaauc auugaaagac aaggccgcga cuaucccuuc     1380

cuccuggaga auaaggauaa acugcucucu auccugaccu uuaagauucc uuacuauguu     1440

gggccccuug ccaaaggaaa caauucccgg uucgcuugga ucaagagagc cacaagccag     1500

gacauucugg augacaacga ugaggacacc cgcaauggca aaauccggcc uuggaacuac     1560

cacaagcuca ucaauaugga uaagacaaga gacgccuuua uuaccaaccu gaucgggaac     1620

gauauuaucc ugcuuaauga aaaagugcuc cccaagcgcu cucugauuua ugaggaaguc     1680

augcugcaga acgagcucac gcgggugaaa uacaaggaca aauacggaaa gacacauuuc     1740

uuugauagcg aacugagaca ggagaucauc aauaaccuuu ucaagaccaa uuccaaacgg     1800

guuucuagcg cuaugcugcu caacuaucug gagaauuaca ccaaccugca agcgguggaa     1860

auugucuccg gcaucgagaa ggggaaaagc cuuaauucua cacucaagac cuauaacgac     1920

cugaaaacaa ucuuuuccga ggaacugcuc gacagcgaaa uuuaccagaa ggagcuggag     1980

gaaauuguga aggucaucac gguguucgau gacaaaaagu cuaucaaaaa uuaucuuacc     2040

aaguacuuug gacgccugga guuccucaac gaagagagaa uuaaucagcu guccaaacug     2100

cgguauacag gcuggggcag auacagcgcc aaacuucucc uggauauccg cgacgaagau     2160

accggguuua accugaugca guuccuccgg aaugacgaug agaacagaaa ccugucccaa     2220

cuuauuucug acagcacacu guccuuugau ucugcuauca agagcauuca guccaagagc     2280

accguugagg acgauguguu cgacgaaauc aagaaacucg ccggaucucc ggccaucaag     2340

cgcggcauuc uuaauuccau caaaauuguc gaugagcugg ugcagaucau uggguacccu     2400

ccccagaaca ucguuauuga aauggcuaga gagaauauga cgaccgagga aggacaaaag     2460

aaagccaaga cacggaagac caaacuugag agcgcgcuca agaacaucga aaauucugug     2520

cuggagaacg gcaaaguccc ucacuccgac gaacagcugc agagcgagaa gcucuaucug     2580

uacuaucuuc aaaaugggaa agacauguac ucucuggaua agacaggcag ccccacgccc     2640

cucuaucgcg acagacugga ucaguacgag guggaccaca ucauuccuua uuccuuccug     2700

ccgaucgaua gcauugacaa caaggugcuu acccaucggg aaaauaacca gcgcaaacug     2760

aauaacaucc ccgauaagga gacaguugcu aauaugaaac cuuuuuggga aaagcucuac     2820

aacgccaaac ugauuucuca gaccaaguac caaagacuca caaccuccga gcggaccccc     2880

gacggagucc ugacagaaag caugaaggcc ggcuucaucg agcgccagcu uguggagacg     2940

agacagauca uuaaacacgu ggcucggauc cuggauaauc gcuuucucga caccaagauu     3000

aucacacuga aaucccagcu gauuaccaac uucagaaaca cauuucauau cgccaagauc     3060

cgggaacuua augauuauca ccaugcucac gacgccuacc uggcggucgu gauugggcaa     3120

acccuccuga aagccuaucc uaagcucgcu cccgagcuga ucuacggaca ccaugcccac     3180

uucaacagac augaagagaa uaaggcuacg auucgcaaac accuuuauuc uaacaucaug     3240

cgguuuuuca auaacccgaa uagcaagguu uccaaagaca uuuggaacug caauagagau     3300

cugccuauca uuaaggacau caucuacaac ucucagguga auuuugucaa acgcacaaug     3360

auuaagaagg gcgccuucua uaaacagaac aucauuccca augauagcuc cgugaccguu     3420

gacaagcggu uuccuaucaa aaacaagaaa uaccucgauc ccaagcugua cgggggauau     3480

uacgaaagaa auucugcgcu gagcguguuc auuaucggcc aguccuucaa caaaaauaac     3540

aaacuuaagg ucgugaagga guuuuaugac aucaacauua ucgauuaccc ucuguucaau     3600

accgacccca ugagcuuuuu caacgaugac ucugagaaug gguuucucac aaagaacaau     3660

cugcaauccg uucaggccuu cuauaaaauu ccgaaguaca gccucaucca gaaaaacgau     3720

ggcacccgca ugcuguuuga aucuacaacg aauuuccaca aggcuaccca auuuaaacuu     3780

acagaggccc agaacgaacu guucuuucau augaagcggc ugcucaccaa gacagacuuc     3840

auggaucuga aauccgagga gagcauuaag gaaucucagg acuuuaucau gaaucacaaa     3900

aacgaguucg acaauaucgu gaacggacuu uucggcuaug cugaaagaca gcuggggucc     3960

aagagccucu uuacccaagc ccaugaggcc uacaagcugc gcaaauaccc ugucuucucc     4020

cucaaugaag agacgauucg guauuacuau gauaacuuuc ugagacuuuu cagccugacc     4080

aagucuggaa caagcuccgg cuacguggug gggcuguuug aggcucccaa acuccggaau     4140

gucguuuauc gcgcgaccaa ggacgaaucu aacgccacac ugauccacca gagcauuacc     4200

ggacuuuacg agacaagaau cgaucugucc aaacucggcg aagacuga                  4248


<210>  4474
<211>  4248
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4474
augaaccuga gaaauguggc caaggagggc aucaacuaca auauugggcu cgaccuggga       60

accggcagcg ucgggugggc uguuacagau gaacagggag agcuuuucuc cuuuaaaggc      120

aagacgaccu gggggucucg gaucuucccc acagccgaaa ccgcggcuga cacacgccug      180

aaaagaggac aacggcgcag auaugcccgg cgcagauggc ggcucgaucu gcuucagagc      240

cuguuugagg aagagauuaa gaaaguggac ccugaguucu uuacccgccu cagacagucc      300

caucugcuga aggaagauaa gacauacgcc gcuauccucu cuagcaaagg cgccacccgg      360

ccgcuguucc ccgagggcga agcugaggug gaguauuaca agaaauaucc uacgauuuac      420

caccuucgca aggcccugau ggaauccaac aaucaggagg acaucagacu caucuaucug      480

gcguuucaca acauugucaa agcccggggg aauuuccuuc aucaagauac ccccggacug      540

acagcuucua acgccaauau gggcgacgcu guggaaaagc ucugcagcgc ccuggaggau      600

ugguguucca gccagaacau cgaaugcgcg auucgcaaga aucagaaauc uauccaggac      660

cugauggagg ggaagaccau ugagccuucc gaaagcaacg cccucaaaga gacauuugau      720

gcuucuagau ccagccggaa ggacaaagcc ucuguucugg cuggauggau cgccauucaa      780

cccggcgaac aguucgagcc uaagcaggcc aaagaugcuu ccaaggaaau cgcgaaugcc      840

guguuugggc uuagcgcuaa caugaagaaa gucuucgugg agcuggagga agaccagaag      900

aucuaccuca auaacgacga gcaaguugaa gccuuuuaca ccgugugucc ggaugaggga      960

gucgcccugu ucgaagcccu ucaguccaug uauucugcuu acgugcugau gggcauucuc     1020

aaaggcgcgg acgggggaag caucuccagc ugcaaggugg cugccuauga gcaguacaaa     1080

gaggaucuga agcugcucaa gacgcugguc agagaguucg cccccaaaaa guaugacgag     1140

uuuuuccgcg gcgauauuuu ugacgcuaaa aagggguucc ggacaagaaa cuacgauccu     1200

gccaccgcua aaggauauac aaaguacaau ucuguucgcg gcgccuccua cgacgcguuu     1260

aagaaagaag uggagaaacu uuucgagggg accgccgcug aagaggaugu gcgguauacc     1320

gacaugaugc aaagauuuga ugaaggaaca uuccugcgcc ggcucaagac gagcgacaac     1380

ggcucuaucc ccuaccagcu gcaccuugag gaaauggucg ccauuaucga gaaucagaag     1440

accuuuuauc cuuuccugga ggaaaacaaa gagaaguuug aaagccucgu gacauucaga     1500

auccccuacu auguuggccc gcugacccag aagaaugcuc gcccugacaa ccaugggaau     1560

gcccgguuug cgugguccca aagaaaagag ggacaggaaa acgcccgcgu guaccccugg     1620

aauugggagg aggucauuga ucggaagcag ucugcugcca aauucaugga aagacugaca     1680

ggcaccugua cguauauccu cgaggaaccu gugcugcccc gguacagccu ucuguacgag     1740

gaauauugcc uccugaacga gcugaauggg gcucgcugga gagacauggg aggcgauugg     1800

caccgguucg acuacgagac aagaauggau auuaucgaag accuuuuuaa gaaaaagaaa     1860

gggaccguua aguaugccac cguggccgau uggcucgucc gcgagaagca ccgguccugu     1920

gcugaaguga aaggcggaca gggcgagaca ggguucgaga gcaagcuguc uuccuacguc     1980

uuuuucaaag gagugcugca uguugacgaa auugaugagg cggacauccc caugaucgaa     2040

gaacucauuu gguggaacac ccuguuugaa gauagaagca uccuuaagga gucuauugag     2100

gacaaauaug gcgaucgccu gaaugacgaa caaaucaagg ccauuugcaa gaaaagauuc     2160

acaggguggg gacggcucuc caagaaacug cuguguggca ucaaggcuca gacggacaac     2220

gggccuugca gcauuaugga ugugcuucgc gagggcaauc cgaacaacga cgccagaucc     2280

gccaccaugg ucuuuaugga aaucauccgg gaugacaaau ucggauuuac aaagcucauu     2340

gaggaagaga auaagaaacg caucucuagc guggaugacu ucgauguuaa cgaccugccc     2400

ggcuccccug cugugagacg gaccgucaau caggccaagc gcauugugga ugagaucgug     2460

gggauugcgg gaugugcucc ccagaacauc uuuaucgaag uuacaagaga cgaggauggc     2520

cggaauaaag ggcgcagaac caagcggaga uacgacgcca uucaagaagc cgucaaagug     2580

cugaagaagg agcagaaaga auauuucgau gcuaacgugg ccgaccuccu gaagaauacg     2640

gcgccugcug cccuugacga ugagcgccug gcccucuacu uuaugcagaa cggaaaaugc     2700

auguacugcg gcaaggaccu ggauaucaau cagcuggaca aauaucaagu cgaucacauu     2760

cuuccccagu cuuacgugaa ggacgauagc uucgagaaca aagcucucgu ucauuccgac     2820

gauaaucaga gaaagagcaa cucuaugcug cuggacgauu ccaucauucg gaagaugaaa     2880

ccguauuggg aagcccuccg cagcgcuggg cugaucggcg agaagaaauu caagaaucuu     2940

acccggguga gagucucuga caaagaacug ggaggcuuua ucgcccgcca aaugguggag     3000

acaucccaga gcaugaagcu caccucucug cugcuugagc aggcguaccc ugaaacacag     3060

guucggcccg ugaaggccgg guucagcucc gaccucagac gccggcugaa acuggucaag     3120

uguagagaga uuaacgauua ucaccacgcu caugacgccu accucgcuug cgaaguggga     3180

caauuuaucg cccugcggca caauaaacuu uaugagaacc cuauuggcua cagccaugcc     3240

guuaaggauu ucaucgcuaa acgcgcgaag uacgccucug aacacgggaa gcccauggac     3300

accuauggaa ugccuggcuc cagcuccuuu gugauuucua gcuucaugac guccggguuu     3360

gaugaggaga caggcgaaau cuucaaagac gcuugggaug ccucucuggc cgcugucaga     3420

auccaucagg cccucaaccg gaaggacugu uacauuagcc gcaugcccga ggaaaccucc     3480

ggagcguuuu gggaugcuac aaucuauucu ccgaaacaga ccaagaaaaa gcugagccug     3540

ccucuuaaga aaggccugga cccccagaag uacggguccu auagcagaga gcaauucgcc     3600

uacuuuuucg uguaugaagc caaaaagauu aaaaagaagg aggagaccaa agaauacgug     3660

uucgcuucug uccccguuca gguggccgcg cucguggcug ccgauccuaa ugcccugauc     3720

gaguuugcua aggccgacgc ugaggaacgg ggacucaaau ucgccaagau ugucagagaa     3780

aaaguguaca aguaucagcu gaucgagguu aacgaugagc gcuuuuucgu gacaggccuu     3840

cggacgguca gaaaugggcu gcagauugcg uuuuccgaac aagaccgcaa caucgugagc     3900

ggaaucguug agggcaauuc uuccaacaau aaccuccugg acauuuacga uaaaaucacc     3960

aaucgguaug cccuguacgc uagcagauuc ggcaaacuuc ugaaccucuc uggguaucgc     4020

gaaaauuuua uucaggccuc ccuggaggac caggagcggg ugguccucgc ucugaucagc     4080

cuuuucaacg ccagaacaaa uaugauugau cuguccgcca ucggaagcuc uaagaccgug     4140

ggcaacauga gcaugacauu ugcuaaggaa auggcgaugg ccgacaaucg cuucgcuuuu     4200

aucgaucaau ccguuaccgg gaugcucgag agaaagacgu accuguga                  4248


<210>  4475
<211>  4251
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4475
augggcagca gaaucauucc caugucccag gacauccugg gggaguucgg aaagggcaac       60

ucugugagcc aaaccuccga acggacagcc uaccgcucua cgagacggcu ccgcgagaga      120

uuucugcuuc ggcgcgaaag acugcaccgg guccucaaua uucuggauuu ccuuccuacc      180

cauuaugcua gccagaucga cuuuaaaaag cgccugggga aauucaagga gaacaaagaa      240

ccgaagauug ccuacgauaa uaaguccuuu cucuuccugg acucuuuuaa cgagaugcug      300

aaagauuucg agcaggaaca gcccaauaug cucaucaacg agaagggaga aaagaagcug      360

aucccuuaug acuggacacu uuacuaucug cggaaaaagg cgcucagaga gaagauuagc      420

aaagaggaac uguccuggau cauucuuaau uuuaaccaaa agcgcggcua cuaucagcug      480

agaggggagg augaagagga aaaucccagc aaacucguug aguuccacuc ucugaccgug      540

guggacgucg ugccugauga gcccaucaag ggaaaacugg aaacauggua cuccauuauc      600

cucgacaacg gcuggguuua ccggcgcagc ucuaagaccg agcuguauga uuggaaagac      660

aagauuaagg aauuuaucgu gacaaccgau cuuaauuacg acggcacggu caaaaccgac      720

aaggagggga acgugaaaag auccuuccgg gcuccuagcg aaacagauug gacccugauc      780

aagaaaaaga cagaguauga gauugaacag ucuuccaaga ccaucggaga guacauuuau      840

aauacgcucc ugaaauaccc ggaacagaag aucagaggca aacuuauucg cacaaucgag      900

cggaaguauu acaaagagga acugaagcaa auccuccuga agcagaccga guuucauaac      960

gaacugaaag acgauagccu cuacuaugac ugccugagag agcuuuaccg caauaacgaa     1020

ucccaccagc uguuccucga gaagaaaaac uucgcccagc uguuuauuga ggauaucauu     1080

uucuaucaac ggccccuuag aucucaaaag agccugaucu ccaauugucc ucucgaauuu     1140

uacaaauuca aggacagcgu ugggaacgag aaggugcagc aucugaaagc cauucccaag     1200

ucuaauccuu uuuaucagga guuccgcauc uggcagugga ucuccaaccu gagcauuuac     1260

ucuaaacucg augacagcaa uuuuacaucc cacuucuuug auucuaccga cgauugggaa     1320

agccuguucg aaaugcuuaa cggacggaag gagaucgagc agaaacagcu gcucaaguuu     1380

cugcuggaga aaaagggcuu caaggggaaa ucccuuagcu cugaaauuga aaaguauaga     1440

uggaauuacg ucgaggacaa aaaguacccc ugcaacgaaa cccgcucccu cauccaaagc     1500

cggcuggaca aaguggcuaa ucugccggag ucuuuucuca caagacacgu ggagcaggaa     1560

cuguggcauc uuauuuauuc cgucacggau aagaucgagu ucgaaaaggc ccuggacacc     1620

uucagcaaaa aguacaaaau ugauacaccu accuuuguug acgcuuucaa gaaauguccc     1680

ccuuuuaagu ccgaguaugg aucuuucagc gaaaaggcca ucaaaaagcu ccugccccug     1740

augcgcacag gcaaauacug ggaucuugac aacaucgaua aggcgaccaa acagcggauu     1800

gagaagauca uuuccggcga guaugacaag gauaucagag accgggugcg cgaaaaagcc     1860

auuaaucucc ugucugagac ggauuuuaac agacugcagc ucuggcuggc ucaauacauc     1920

guguaugacc ggcacagcga agccacaucc cuugauaagu gggagaccau cgacgacauu     1980

gucaccuacc ugcauagcuu caaacagcac ucucucagaa aucccaucgu ggagcagguu     2040

auuacagaaa ccauccgcgu ggucaaggau auuuggucca acuacgggaa uggaagcaaa     2100

gacuauuuua acgagaucca cguggaacug ggccgggaga ugaagcugcc ugcugaagag     2160

agaaaacgca uccaggagaa gaacucucaa aaugaagaua caaaccuuag aauuaaggcc     2220

cuccuguccg agaugcagaa ugacccgaac guugaaaaug ugcggcccua cagcccuguc     2280

cagcaggaga uccugaaaau uuaugaagag ggggugcuca acucuaauau cgagauugcg     2340

gaugacaucc ugaagaucag caaaacggcc caacccucca gcucugaucu uaagcgcuac     2400

aaacuguggc ucgaacagaa guaucagucc ccuuacaccg gagagauuau cccccugaac     2460

aagcuguuca caccggaaua ugagauugaa cauaucauuc cucaaagcag auacuuugac     2520

gauuccuaca auaacaaagu caucugcgag ucugcuguga auaaacuuaa agacaacagc     2580

cucggcuaug aguucauuaa gggguacaau ggacagaucg uugaaugugg cuuuggcaaa     2640

aaggugaaga ucuucuccau ugaugaguau gaagacuuug uccagaaaaa cuacgccaau     2700

aaccggucua aggcuagccg ccugaugaug gccgauaucc ccgagaaaau gauugagaga     2760

cagcugaacg acacccggua uaucuccaag uacauuucua auauccucag caacaucgug     2820

cgcuccgaaa gcaaugacga ugggguuaac ucuaaaaauc ugauuuccgu gaacggaaag     2880

aucacaaccg uccuuaagaa agacuggggc augaaugaua uuuggaacga ccugauccuc     2940

ccuagauuca uucggaugaa ucaaaucacg gauagcgacu cuuucaccac auggaacgag     3000

cgcuuucaga aguucauccc caccgugccu auugaacugu ccaaaaauuu uagcgccaag     3060

agaaucgauc accggcauca cgcuauggac gcgcugguga uugccugcgc uacaagagau     3120

cauguuaacc uucugaauaa ccucuaugcc aaaucuuccg cccgcuacga ccugaaucgg     3180

aagcucagaa ucuucgagaa gaccacguac aaagauauua agacagggca gaucauugaa     3240

cgcgagaucc ccaaggcuuu ucugaagccg uggcagaacu ucaccacaga cgccaaagac     3300

gcgaucgaga gcauuaucgu cuccuauaag caaagccuuc gggugauuaa caaaucuacc     3360

aauuacuauc agaagugggu ggaaaaggau ggaaucguca aaaaggagaa aauuaagcag     3420

gaaggcaaaa acugggcuau cagaaagccu cugcacaagg acaccgugag cgggcucguu     3480

cagcuguccu gggugaaagu ccccaagggc aaagugauga cagccacgcg caagucucug     3540

gauagcuccu uugaccuuaa aaccaucgcc acaauuaccg auacaggaau ccaaaagauu     3600

cugaucaagu accucgaguc uaaaggcaau aaccccgaaa gcgcuuucuc cccugagggg     3660

auugaggacu uuaauaacaa uauccaccug uauaacgaug gaaagaaaca ucggccgauc     3720

accaagguua gacucuacga aauuggcucu aaauuccccc ugggggagaa gggaaauaag     3780

aacgccaaau auguggaagc ugccaagggc acgaaucugu uuuucgcggu cuacgccaac     3840

aauaaagggg agcgcagcuu cgaaacaauc ccucuuaacc ucgugauuga ccggcugaag     3900

cugggccuuu ccgcuguucc cgagaccaau gagaaaggag auaaccugcu cuuucaccug     3960

agcccuaaug accucgugua cguccccucu gaagaugaga uggaaaaccu gaaacuuauc     4020

aaccuggagc auauuuccaa agaccagcug aauagaaucu auaagauggu gagcucuacc     4080

uccaaccggu guuucuuuau uaagagcgag aucgcccucu ccaucgugaa uaaaauugaa     4140

uacucuccuc ugaacaagau ggagaaaagc aucgaaggca uuaugaucaa ggaugucugc     4200

cuuaaacugc agguuaaucg cauugggaac cucgagagau auuucuccug a              4251


<210>  4476
<211>  4257
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4476
augaagguga aaaacauccu gggccucgac auugggguca ccagcaucgg auucgcccac       60

guuacagaga augaaaagga uaacaaaucc cauauuaagc agcugggcgu gagaaucaau      120

ccccuuacgg uggacgagca aaccaacuuu gaaaaagggc ggccugcuuc ugucaaugcc      180

gaucgcacac ugaagagagg agcgcggaag augcucagcc gcuacaaaga cagacggaag      240

aaccugauug agauccuuuc caaaaccggc uucaucaauu cugaaagcau ucuggcugag      300

gaugggaaga acacaaccca cuccacaugg gcccuccgcg ccaaggcuuc uagagagcgg      360

aucgaaaagg acgagcuggg acgcgugcug cucgccauua auaagaaaag aggcuauaag      420

agcucccgga aagcuaagaa cgaagaugag ggccaggcca ucgacgggau ggcgauugcc      480

aaacgccugu acgaggaaaa ucuuaccccg ggacagcugg uuuaucagcu ccugagagag      540

ggcaagaaau acauccccga uuuuuaucgg agcgaccuuc aagaugaacu ggacaaggug      600

uggaacuucc agaagcaguu ucaucaggag auucucacgg auaccuucua caaagacauc      660

caagggaagg gacagcgcgc uacaucugcc uccuuuuaug gcgucuacgg guucaauacc      720

gcugaaaaca aaggaagcag agaggagaag cggcugcagg ccuacgcgug gagaucugac      780

gcccugacaa aacagcucac ccgcgaagag guggcuuuug uuaucacgga aauuaauaac      840

aaccugaaua acuccagcgg cuaucuuggc gccaucucug aucgguccaa ggagcuguuc      900

uuuaauaaga aaacaguggg gcaauaccuc uaugaacagc ugcagcaaaa cccucacacc      960

agacuuaaga aucaggucuu cuaccgccag gacuaucugg augaguuuga gacaauuugg     1020

accauccaga gccgguacua ucccgaacuc accgacgauc ugaaaagaga gauucgcgac     1080

guggugaucu ucuaccaacg gaagcugaaa ucccagaagg gacucgucuc uuucugcgaa     1140

cuggagagca aggaaaucau gauugagaaa aacggcaagg cugccguuaa aaagaucggg     1200

uccaaagugg ccccuaagag cucuccccuu uuucaggagu ucaagauuug gcaagugcug     1260

cauaaugucg auaucaaaaa caagaaagac aagacaaaaa gaccucucga ugacuccgaa     1320

aaggaguacc uguuuaauga acuuaacauu aaaggaaauc ugaaggcugc gaaggugauc     1380

caacuccuga cgcugaacaa aaaugagacc gagcucaacu auacacagcu ggaaggcaau     1440

cgcacccaga aggcccuuua cgaggcuuau cugaaaaucc ucgauacaga aggguacgac     1500

guuaacaacc ugcugggaau uaagagcaau aaagacgagg uguuccuuga ugaccucgau     1560

gucaacgccc uggaaaucaa gaccaugauu aagcagaucu uuaaugcccu gaacauuaau     1620

acgggcaucc ucgacuucga ugcugagcug gacggcaaag ccuuugagca acaggcgucu     1680

uaucaccuuu ggcaucugcu cuacagcuau gaagaggaug gguccaaguc uggaaacgaa     1740

caccuguaca aacugcuuga gaauaaguuc ggcuuuaaac cggaacacgc ucggauucuc     1800

gccgggauca cacugagcga cgauuacgga aaccuguccg ccaaggcuau gagaaagauc     1860

uauccccaga uuaaagagaa uaacuucagc accgccugug agcucguggg cuaccgccau     1920

ucuaaaaauu cccugaccaa agaagagaac caggaacggc cucuuaagaa uaaacuggag     1980

cuccugaaga agaacagccu gagacaaccc guuguggaaa agauccuuaa ucagaugauu     2040

aaccucguca acgagcugau cgacaaaaau ucugagaaag augaacaggg guccauuauc     2100

gaguauuuuc gguucgacga aauccgcauu gagcuggcua gagagcucaa gaaaaacaca     2160

gaugaacgga gagaccugga cgccgcgauc cgcgagggca aucuucggaa cgaaaagauu     2220

uacaaacugc uccaagccca guuuggaauc aagaauccua ccagaaacga uauuauccgc     2280

uauagacugu acgaggaacu gaagaauaac ggcuauaaag accuuuacac aaauagcuac     2340

aucccccagg agaaacucuu cgggaaagag auugauaucg accacauuau cccgcagucc     2400

aagcuguucg augacucuuu uagcaacaaa acgcugaccu auaauacaac caacaaggau     2460

aagggaaauc aaacaggcuu cgacuacauu gaauccaaau augggaucga uggaaucgag     2520

gcuuacaccg cccggauuga aaaccucuuu gagucuggca agaaaaauaa ggaagagggg     2580

gugagcaaag cuaaguauca gaagcugcuu aaaaaggagu ccgaaaucgg cgacggauuc     2640

auugagcgcg aucugagaga aagccaguac aucgccaaaa aggcgaaaga gcuccugcug     2700

cagauuacgc ggucugucgu ggccaccucc ggcagcauca cagcucgccu uagagaagac     2760

ugggaucugg uuaacgugau gaaggagcuc aaccugccua aguaucgggc ccucgggcug     2820

accgagaugg aggaacgcaa auacggacaa aaggucgaag ugauuaaaga cuggacaaag     2880

agagacgauc accggcauca cgcuauggac gcccuuacca ucgccuuuac gaaacauaau     2940

cacauccagu accugaacca ucucaaugcu cgcaaggaug agcagcacaa ggagcacaaa     3000

auuaucaacg cgauugaaaa uaaggagaca auccugauua ccgacaaauc uggcaacaga     3060

aagcggguuu ucaaagcccc caugccuaau uuuagacaga cagcuaagca auaucuggaa     3120

gaggugcuua ucucccauaa agccaagaac aaggucguga ccaaaaauaa gaacaaaauc     3180

cuggggaagg aaaagauuca ggagacccuc acaccccgcg gacagcugca cgaucaaacg     3240

aucuacggcu ucaauuggug gagccggauu acccucucua agaaggugag cagaaacgag     3300

aucgagaaaa uuaucgacaa uaacacaaag uuuuuccugc aggaauuuau ccagcuuaaa     3360

ggguccauug ccaaggcuuu cagcgaugcc gaacugaaua aacucaugua uaagggcaag     3420

auccugaaag agauugaagu ugcgaucccc ugcuacaccc agcgcauuga caucucuaag     3480

gucuuuacag auucccagaa aagcaacucc gcuaaggaga aaaauauuaa gaacauccug     3540

gaccuuaagg ugcggaacau ccugcagaau agacucucug aguucaaagg agauuucaag     3600

aaagccuuua gcgaccugga caagaacccu auuugguuca auaaagaaaa gggcaucucc     3660

auuaaacgcg ugaucauuuc uggggucaag aacguggagc cgcuccauua uaagaaagau     3720

caccugggaa augacauccu ugauggcaag gaaaaccgga uccccguuga cuuuauuagc     3780

accgggaaua accaccaugu ggccaucuac caggaugaga aaggaaaucu gcaggaaaag     3840

gucguguccc uguucgacgc uguuaaacuc gugaacgccg augagccugu cauugacaag     3900

acguauaauc aaggccuggg guggcaguuu cuuuucacaa ugaagcagaa cgagauguuu     3960

guguucccca augauaacac cggcuuugac ccuaaagaaa ucgaucugcu cgaccccucu     4020

aauaagaaac ugauuagccc gaaccucuuc agaguucagu ccaucagcau cgugcaauac     4080

ggaaacaaua uuguccgcga uuuuaaguuc cggaaccacc uggagucugu gcuuaaugac     4140

gacaaagugc ugcauggcau caccuuccag cagauuaagu cccuggggcc ucuccugaac     4200

aucgucaagg uuagaauuaa ucaccuugga gauaucguga gcgugggcga auacuga        4257


<210>  4477
<211>  4260
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4477
auggccaagc cccagaacua caccaucggc cuggacauug ggacaaauag cgugggaugg       60

acggucaccg augaccaaaa caauauccuc agaauuaaau ccaagaaagc uaucggcgcc      120

cggcuguucg aggaagggaa gguugcggcu gagcgcagau cuuuucggac aacccgcaga      180

cggcuuagcc gcagaaaaug gcggauuaag cugcucgaag aacuguucga ugaagagauc      240

uccaaggugg acccuaacuu uuucgcccgc ucucacgaga gcuggaucuc cccgaaagau      300

aagagagaac gguauucugc cauuguguuu cccacaccug aggaagacaa aaaguuccau      360

aaugaguacc ccaccaucua ucaccuucgc gauagacuga ugaaagacga ucagaagcau      420

gacauucggg agaucuacgu cgcuauucac cauaucauga aguaucgcgg aaacuuucuc      480

cacgaugaaa gcauugagac auacagaucc ggcaugagcu cucuuggggg aggcgacgaa      540

cggaccaucc ugaauacgaa aauccucgau gaccugaacg acgccuucgc ugauaaucuu      600

gccacccagg gcauuuccgu gaacagcgcg ucugccgacc agaucaauga gauuguuaca      660

uccgggcacc ugaacgugga uagccaaaag gcuaucgcca aacuccugcu uccuaccaca      720

cugucuuccg acgauaagaa acaggacaag aaaauuaaga agcucgcuac cgaugccgcg      780

aaaaaugucg ccagcgcuug gcugggauau aaggccaacu uugcugccau ccugaauauc      840

caggaagugg acaaagauaa gaaaaagauu uacuccuucg cccuccaggg cgcugacgag      900

gaggauaagg uugaaacgcu ggagucugug cuuagccaag accagaucga aauugucgac      960

agaaucauuc gcauccggca ugcgaucgug cugggggaga uuauccccgu gggaaugaca     1020

cucuccgaag ccaugauuga gaaauacgau cagcacgcua aggaccugaa aaaccuuaag     1080

accgucauca gaaaccugaa agaucgcaag aaugccguua agcuccaggc cauuuaugac     1140

cuguacguga acaaucggca ugcugauaca gccaaagcga ugaagcuggc uggcgugaaa     1200

aagagaaccg agcuccugga accugaggaa uuuaacaaaa gcaucgagaa gcugcuggac     1260

gauucuccgg ucuccgccga aauuaagaaa cgcaucgccg agaaggacuu ccucccccug     1320

caacggagca auucuaacgg ggugaucccu aaucagcuuc accagauuga gcuggaugaa     1380

aucauuaaaa agcaaagcaa auauuacccc uuucucggag agaccaacga acacgcuucc     1440

gccgacguug cuagaaaggc cccuacaaaa cuggaucagc ugcucacguu ccgcgugccc     1500

uauuacgucg gcccgcugau caccaaggag gagcaggagg agcaggacgg ccauucuuuc     1560

gcguggauga uucggaagaa uccuaaagau cacgaaaaga ucacacccug gaacuuugac     1620

gagaaagugg acagaauggc cagcgcuacc caauucauca agcgcaugac aaccaaagau     1680

acguaucuuc ugggggaaga cguucucccu uccagcucuc ugaaguacca gcuguuuaca     1740

gugcuuaaug agcucaacaa ucugcggauu aaccauaaga aacugaccag agaggaaaag     1800

gagcagguca ucgaagaucu cuucaaaaag uacaaaaccg ugaaggccga caaguuuaaa     1860

aauuauuggc agaagggaca cgcuggccug gaugucaaaa uucaagggau ggccgacccc     1920

gagaaguuca acuccacaau gagcaccuac aucgaucuuc agaaaauuuu uggagaccag     1980

cuggaugacg cgcagcggca aaacgauuuc gaaaagauca ucgaguauuc uacaauuuuu     2040

gaggaccgca agauccucgg cgaaaaacug agagccgauu ucguguggcu guccgaggac     2100

cagauuaagc ggcuuugcag acuccgccug cagggguggg gacggcugag ccucaaacug     2160

cuuacgagac ugaccgacgc ugauggccag aauguucucg aacgccugcu gaacuccaau     2220

gacaacuuua ugcaaaucgu gaaggauccc gucauuaaag ccaagaucga gcagaaaaau     2280

cagaaacuuc ucagagcuga cuggucugaa aacgccacag agagcauccu ggaugaggcc     2340

uacaccuccc cucaaaauaa gaaggcuauu cggcaaguga ugcgcguugu gcaugacauc     2400

gucaaggcgu gugauggcaa ccugccggcc aaauucgcua uugaauuugc cagaucucag     2460

gacgaugacc ccaagcggac aguggcccgc gcugauagac uccagaaaau cuaugcccaa     2520

auuagcgacg aggugguuuc ccaggggguc aaggaccagc ugagcggaau gaagucucuu     2580

aaagaucggu acuaucugua cuucaugcag ggcgggcgcg acgcguacac cggaaagccu     2640

cucaauaucg auagacuguc cgacuaugau auugaccaca ucaugcccca gagcuucauc     2700

aaagaugacu cucuggauaa ccgggugcuu gugacgcgcg cugucaauaa ccagaagucc     2760

gacaaugugc cuguucuccu guacggcaac caccuggugg ccgaucucgg gauuaccauc     2820

agagaaaugu gggaccggug gcagaaaagc ggcaugauuu cuaagagaaa gcuggccaau     2880

cuucugacag aucccgcuac ccucagcgag uaucgcgccg agggauuuau caaccggcag     2940

cuggucgaaa caucccaagu gauuaaacug accgcgacga uccuucagag cgaguucccg     3000

gacucuaaga ucauugaagu uccugcuaaa uacaacucca uccugagaaa gcaguuugac     3060

cucuauaaaa gccgcgaggu gaaugauuac caucacgcca uugacgccua ucuguccaca     3120

aucgucggca acuaccucua ccaaguguau cccaaucugc ggagacuuuu cguuuacggg     3180

gaauuuaaga aguucucuca uggagaggag gaaagcuccc accaggcugu gcgccggguc     3240

aaaucuauga acuuucugga ugaccuccug agaggcaagc acggggauga cauuuauugc     3300

cgcgugaccg gagaggugau cuucagccgg aaagauauca uuuccaagcu gaaacaggcc     3360

uacucuuuua agcagaugcu ugucacacaa gaaguuuuca ccaagaaaag cgcucuguuu     3420

gaccagaccg uguauccuuc ccccgccaga gauagcaaga cacggucugg ccucaucccu     3480

cgcaaacagg ggauggacac ggagauuuac ggcggauauu ccggcaauaa ggaugcguac     3540

uucgugcugg cccaggucgu gaaaagcaag gggcauaccc uccaaguugu gggagucccc     3600

aucagagcuc ugcgggcccu ugcuaacugu ucuaauuacu ccgaacagcu gcucaaaauu     3660

gugaaggccc agguucuguu uaacaagaaa acaggcaagg ccaaaaaggg gaucagcgac     3720

gugcgcauuc ugcuugauaa aaucccguau agacagccug uccuggacgg aucugcuuuc     3780

uacaugcucg gcuccagcac cuauaaguac aaucugaagc aaaucucccu cagccagcag     3840

ucuaugaaau auauucugga cuacaucgau gaccccaacu ucaauaagca cgagaugaca     3900

aaaauugauc agcaaaacga gaaggaaugc cuucugagcg uguaugacga gauccuggau     3960

aaaauggaca aguaccucuc ccuguacgau auuaaugggu uucggaagaa acuucauguu     4020

ggcagagaca aguucaucaa acuggaagug gagaagcagg cggaagccau caaaaacguc     4080

cuccgcggau uucacgcuaa uucugauaug accaagcuga gcgaccucgg ccuguccacg     4140

ucucuugggu ucaugcaggc cagcgaugga auuaagcugu ccgacaacgc cguguuuauc     4200

uaucaaucuc ccacaggccu guucgagcgg agagugaaag ucagcgaccu ccugaaguga     4260


<210>  4478
<211>  4260
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4478
augaugauca agaacauucu gggccucgac cuggggacca auagcaucgg augggcccuu       60

auuaaacaga acuucgagaa uaaguacggc gaaauccugg ggaugggauc cagaauuauc      120

cccaugucuc aagaugugcu cggcgaguuu gggaaaggaa acagcgucuc ccagacagcu      180

gcccggacgg gcuaucgcgg cguuagacgg cugcgcgaaa gauuccuucu gcggcgcgag      240

agacuccacc gggugcugaa ugugcugaac uuucucccug aacauuacgc gucucagauc      300

gacuucgaga agcgcuuugg gaaauucaag uaugagaccg aaccgaagcu ggcuuacaau      360

aacgauggau uuuucuuuaa aaauagcuuc gacgagaugc uuggcgauuu uaaggaccac      420

caguuccauc ugcucgaaaa cgggagaaaa guccccuaug auuggacaau uuacuaucug      480

cggaagaaag cccuuuccca aaagaucgag aaggaggaac uggccuggau ucuccugaau      540

uuuaaccaga aacgcggaua cuaccagcug agaggcgagg acuucgaaga ggaaaaggau      600

cggguguucg uucgccucaa aguggacaga gucguggauu cuggggagaa uaucaaggga      660

aaaauucugu augacguuua cuuugagaac ggcuggaagu augaaaaaca gaucgugaag      720

accgaggauu ggauuggccg gacaaaggaa uuuaucguca ccgagagcau ccuuaaaaau      780

ggggacacaa agagaaccuu uaaagcugug gacuccgaaa aggauuggau ugccaucaaa      840

acgaagaccg agcaagagau ugacaagagc cacaaaacag ugggagcuua caucuaugaa      900

gcccugcucc agaacccuaa gcagaaaauu aagggcaaac ugguccgcac caucgagcgg      960

aaguucuaca gagaggagcu uaagaaaauc cuggaacgcc agaaggaguu ucaucccgag     1020

cuccaaucug augaccugua uaacgauugc auucgggaac uguacagaaa uaacgaggcg     1080

caccagauca cacucuccaa aaaggacuuc guucaucugc uucugaauga ucucauuuuc     1140

uaccagcgcc cucugcggag ccaaaaaucu uccgugagca acuguacccu ugaguucaga     1200

aaguauaagg acgaaaaugg gguggagcac gcccaguacc ugaaaacgau ccccaagucu     1260

aacccuuauu accaggaauu ucgcgucugg caguggaugu ucaaucucaa cauuuaucgg     1320

aaagaugacg auacaaaugu gaccaaggag uuucugucca gcacagaaga cuacgagacc     1380

cuguucgaau uucucaacaa uagaaaagag guugaccaaa aggcucugcu ucgccacuuc     1440

aaacugaacg agaagaccca ucgguggaau uauguggaag auaagaaaua cccgugcaac     1500

gagacaaaga cgaugaucuc caccagacuc gaaaaagucg agaauuaccc cucugacuuc     1560

cugacacggg aaauggagca gaacaucugg cacauuaucu auagcgugaa cgauaagauu     1620

gaguacgaaa aagcccugaa guccuuugcu cacaagaaua accuugacga gaauagcuuc     1680

uuugaagccu ucaaaaaguu uccucccuuc aaaucugagu auggauccuu uagcgaaaag     1740

gcgaucaaaa agcuccugcc ucugcuccgc cugggcaagu acuggaacug ggagucuauu     1800

gauagaaaaa gccaggggcg gaucgagaag auuaucaccg gagaguucga cggcaauauc     1860

aaagauaagg uuagagaaaa agccauucau cuuacagcug aaaacgacuu ucagggccug     1920

caacucuggc uggcccagua uaucguguac gggcgccacu ccgaggcuuc uaugauugga     1980

aaguggaaua gcgccgauga ccuggaggag uuccuuaagg gcuuuaaaca gcauucccuc     2040

cggaacccca ucgucgaaca ggugauuacc gaaacgcuga gagucgugaa ggauaucugg     2100

cugaaauaug ggaauggagc caaggacuuc uuuagcgaaa uccacauuga acucggccgc     2160

gagaugaaac aaacaaagga ugagagaguu gacgcuacca aacggaucac cgaaaacgag     2220

aauacaaacc ugcgcauuaa ggcgcuucug gccgaaauga ugaaugacaa cucuguggag     2280

aauaucagac cguacucccc uaugcagcag gaaauucuca agaucuacga ggaugggauc     2340

cugaaaagcg acauugagau cgaugacgau auucugaaga ucucuaaaac cgcucagccc     2400

uccagcuccg accuuaagcg guauaaacuc uggcuggaac aacgcuacaa gucuccuuau     2460

acaggccaga uuaucccccu gaacaaacuc uucacgagcg aguacgaaau cgagcauauu     2520

aucccccagu ccagauauuu cgaugacucu uuuagcaaca aaauuaucug ugaauccgcc     2580

gucaauaagc ugaaagauaa cuacauugga cuuggcuuca ucaagcaauu ugggggaaug     2640

auuguggagu gcggcuucgg gaaaagcguu gagaucuuua aggccgaaga guaugaagac     2700

uucgugaaga aacacuacgc uaauaaccgg ucuaagcgca auaaacugcu ccuggaggag     2760

aucccugaaa agaugauuga uagacagcug aacgacaccc ggcacaucuc caaauacauu     2820

agcggagucc uuucuaauau cgugcgccag gaggauggca cagacgaagg ggugaacucc     2880

aagaauauug uuccgggcaa cggaaagauc accacacagc ucaaacaaga cuggggccug     2940

aaugaugucu ggaacgagcu gauccucccc agauuugaac ggaugaauca gcugaccaac     3000

agcacggacu ucaccucuug gaauaagaac caucagaaau uucuuccuac agugcccauu     3060

gaguucagca agggguuuuc caaaaagaga aucgaucacc gccaucacgc cauggacgcg     3120

cuggugauug cuugugccac caaggaucac aucaaccucc ugaauaacca gagcgccaaa     3180

ucugacacaa agcgguauga ucugaaaaga aaacuuauga aguucgagcg cgucguguac     3240

ucccauagca agaccggaga aaagauugag aaggaaguuc cuaagcaauu ccugaaaccc     3300

ugggacaauu uuacggugga ugcuaagaac aaaauggaga caaucaucgu cuccuucaag     3360

cagaaucucc gggugauuaa caaagccacc aaucacuaug aaaaguggau cgagaaggac     3420

ggcauuaaaa gaaaggagcu gaucaaacag gaagggacaa acugggcuau ucgcaagccg     3480

augcauaaag agaccguuuc uggaaaggug gaucucccug gcaucaagau ucccaaaggg     3540

aagauccuga ccgccacacg gaaaagccuu gacacguccu uugaccugaa gaccaucaau     3600

ucuauuacag auaccggcau ccagaaaauu cucaagaacu accuggaguu caagggaagc     3660

uccgaacugg cguuuucucc ugaaggcauc gaggacauga auaaaaacau ugagaaguuc     3720

aaugauggga aaccccacca aagcaucaac aaggucagaa ucuuugaacu uggauccaaa     3780

uucagcgugg gccagacagg gaauaagaaa gacaaguaug uugaggccgc uaagggaacc     3840

aaccuguuuu ucgccgugua cgaagauaaa aauggcaagc gcucuuauga gacgauuccu     3900

cucaacgagg ucaucgaacg gcagaaacaa gggcuggacu ccgugagcuu uaagaacaaa     3960

gaggauuucc uccugugccc caaugaccuu guguacgucc cgucugaaga ugagcuggaa     4020

aacauuuaca auaucgacuu uaagaaccuc acaaccgaga agaccggcaa uauuuauaaa     4080

guuguguccu ucagcggaaa ccagaucuuc uuuaucagac aggauauugc uaucucuauu     4140

gugaauaagg ccgaguucuc cacacugaac aaaauggaac gggcgaucga cggcagcaug     4200

auuaaggaga auuguaucaa acugaagauu gaucgccuug ggaacaucgc caagaucuga     4260


<210>  4479
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4479
auggccaagc ccaaagacua caccaucggc cuggauauug ggacaaacag cgugggaugg       60

gucguuacgg acgaucagaa uaacauccuc agaauuaagg gcaaaaaggc uaucggggcc      120

cggcuguuca ccgagggaaa aguggcggcu gaacgcagau ccuuucggac aacccgcaga      180

cggcuuucuc gcagacggug gcgcauuaag augcuggagg aacucuucga cgaggaaauc      240

gccaaggugg auccuagcuu uuucgccaga cugcacgagu ccuggaucuc uccgaaagac      300

aagcggaaac gcuauagcgc uauugucuuu cccuccccug aggaagauaa gaaauuccau      360

gagucuuacc ccacaaucua ucaccuuaga gacaagcuga ugaaggauga ccaaaaacau      420

gauauucggg aaaucuacau ugccgugcac cagaugauca aggcucgcgg caauuuucuc      480

caugacgaga gcguugagac cuauagaucc gggaugagcu cucugggagg ccgguccgaa      540

agaacaauuc ugagcgugca gacccucgag gaacugaacg aucuuuucgc cgagaaugaa      600

ggcacggagg aggucgaacu gaacguggcg ucugccgagc agaucaauga cauccucacc      660

gggggacacc ugaacgcuga cucccaaaaa gaaauuagca aucuucugcu cccuucuucc      720

uuucccagcu ucgaugacaa ggccaaagag aagcagguua aaaagcugau caacaaugug      780

gcuacaaaca uuuccaaggc cuggcugggc uacaaagcgg auuuuucuac cauccucaau      840

cuggccaagg ucgacaaaga ucagaagaaa auuuucgcuu uugcccuuca gggggaagac      900

gaggaggaua aggugcaaga acuggagagc cuccuggaac agucccagac agacaucgug      960

gaucgccuua ucgagauucg gcacgcuauc guccugagcg aaauuguucc ugugggaaug     1020

acccucucug aggccaugau cgacaaguau gaucagcaua aagaggaccu gauuacgcug     1080

aaggccguga ucagaaacac aaaagacaag aaaaaggcug cgaagcucca agccaucuac     1140

gaucuguacg ucaaaaaucg ccacgcugac cuugccaagg ccaugaaacu gaccggcauu     1200

aagaaacggu ccgaacuccu ggauccggag gaacuuaaga aggggaucag cucucugcuc     1260

gacgauagcc ccgaggcugu ggagauuaaa cagagacugg aagagaagac auuccugccu     1320

cuccagcgcu ccaacaacaa uggaguuauc cccaaccaac ugcaucaggu ggaacuugac     1380

gagauuauca aaaagcaguc uaaauauuac ccuuuccugg ccgaaaagaa ucccgaugag     1440

agcgaggaag cgcagaaaaa ggcuccgacc aagcucgacg cccugcugac cuuucggguc     1500

ccuuauuacg ugggccccau gauuacaaaa gaggaacaag aggcccagaa cggccacucc     1560

uucgcuugga ugguuagacg cgauccucag gaccacgaag ccaucacgcc cuggaauuuu     1620

gagaagaaag uggauaagau ggcuagcgcc acccaguuca ucaaacggau gacaaccaag     1680

gacacauauc uucucgggga ggauguccug cccgcgucuu cccugaagua ccaacucuuu     1740

accgugcuga acgaacuuaa uaaccugaga gucaauggaa agaagcucac gagcgacgag     1800

aaagaacagg ugauugaggg ccuguucaag aaacagaaga caguuaaggc cgacaaauuu     1860

gugaaguauu ggcaggcuaa acauaucggg gccgauauua aggucaaagg acugucugac     1920

ccuuccaagu ucaacagcac cauguccacc uacaucgauu uuaagaaaau uuucggcgac     1980

caacuuaaug augugaaccg ccagaaugac uuugaaaaga ucaucgagua cucuacaauu     2040

uucgaggauc ggaaaauccu cgaagacaag cugagagcug auuuugccug gcugagcgag     2100

gaacagauua aacagcucug ccggcugcgc cuucaagggu ggggaagacu guccgacaag     2160

cuccugaccc ggcugacaga ugcggacggc cagaacguuc uugagagacu cuggaacucu     2220

aaugauaacu ucgugcagau cgucagcgac ccgaauauua aagccaagau cgagcaagaa     2280

aaccagaagc ugcugcgcga cucccagagc gauaaugcug uggagucuau ccucgacgau     2340

gccuauacga gcccccagaa caaaaaggcu auucggcaag ugaugagagu uguccacgac     2400

aucgugaaag ccugugaugg caaugugccu gccaaguucg cuauugaauu ugcgcgcucc     2460

caggaggacg aucccaaaag aaccacagcc cgggucaacc agaugcagaa gaucuacgac     2520

caaauuucug augaaguggu uucccagggg gugaaggagc agcugagcgg aaugaaaucu     2580

cuuaaggacc gcuauuaccu guauuucaug cagggcggga gagaugcuua caccggagcc     2640

aaacucaaua ucgaccggcu gagcgacuau gaugucgacc auaucaugcc ucaauccuuu     2700

gugaaggaug acagccugga uaaccgcguu cuuguggcca gagcugucaa uaaccagaaa     2760

ucugacaagg ugcccgcccu ccuguucggc aauaagguug uggcggaucu ggggauuaca     2820

guccgggaga ugugggacaa auggcagaag cucggcauga ucuccaaacg caagcugagc     2880

aaccuucuga ccgauccgga cgcucucacg gaauacagag cccaaggauu uauucggcgc     2940

cagcuggugg agaccuccca ggugaucaaa cugacagcca ccauucuuca gucugaguuc     3000

ccugauagca agaucauuga aguccccgcu aaguacaauu ccaucguuag aaaacaauuu     3060

gaccuguaua agucucggga agugaacgau uuccaccaug ccaucgacgc guaccucagc     3120

acaauugugg gcaauuaucu guaccagguc uauccuaacc ucagacgcau guuuguguac     3180

ggggaguuca aaaaguuuuc cucuaacgcu gaggaaagcg cccacgacgu ugcccggaga     3240

gugaaaucca ugaauuuccu ggaugaccuu cugcgcggaa cccacggcga uaacaucuau     3300

ugccggagca cgggggagau ugucuuuaau agaaacgaca ucauuucuaa gcucaagcag     3360

gcuuacuccu ucaaacagau gcuggugaca caagaaguuu ucacccgcaa gagcgcccug     3420

uuugaucaga caguguaccc cucuccugag cgggacucca aaaagagaag cggacuuauc     3480

ccccgcaaaa agggcaugga uccggaaauc uaugggggcu acgcucauaa ucaggacucu     3540

uauuucgucc uggccaaaau ugagaaggug aagggauaca cccuccagau cguuggcgug     3600

ccuauucggu cccuggagau cauuaacaaa gcggaagauu uuagcaccgc ccuccuggac     3660

aucaucaagu ccaaagucau guauaaugag aagacagggc aacccaaaag aggaauuaag     3720

aacguggaaa uccuugugaa uaagcugccc uaccggcagc cuauucucga gaaagauguc     3780

agcuauaugc ugggcucuag cgcuuacaag uacaacauga aacagaucac gcugucccgc     3840

cagucuauga aguauauucu ugacuacauc gaugacccga auuucaccaa acacgagaug     3900

acaaugauug acgaacaaaa cgagagagaa ugucugcuca gcguuuauga ugagauccug     3960

gaaaaggugg acaaguaccu cccccuguuu gaugggaaua aauuccggga gaaacuucau     4020

uccgcccgca aagaguuugu gucucugagc gucgacgaug aggcuuccgc caucgaaaag     4080

auucugagag gacuccacgc caacucugaa augccuagcc uggagaaucu uaaaaucaag     4140

cccgcucugg gcuucuuuca gaugaagaac gggauuaccc ucuccaacga cgcggugcug     4200

cucuaucaga gcccuacagg ccuguucgaa cggagaguua aagugucuga ucuucugaag     4260

uga                                                                   4263


<210>  4480
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4480
auggccaagc ccaaagacua caccaucggc cuggauauug ggacaaacag cgugggaugg       60

gucguuacgg acgaucagaa uaacauccuc agaauuaagg gcaaaaaggc uaucggggcc      120

cggcuguuca ccgagggaaa aguggcggcu aagcgcagau ccuuucggac aacccgcaga      180

cggcuuucuc gcagacggug gcgcauuaag cugcucgaag aacuguucga cgaagagauc      240

gccaaagugg auccuagcuu uuucgccaga cuucacgaau ccuggaucuc uccgaaggac      300

aaacggaagc gcuauagcgc uauugucuuu cccuccccug aggaggauaa aaaguuccau      360

gaaucuuacc ccacaaucua ucaccugaga gacaagcuca ugaaagauga ccaaaagcau      420

gauauucggg agaucuacau ugccgugcac cagaugauca aagcucgcgg caauuuucug      480

caugacgaaa gcguugagac cuauagaucc gggaugagcu cucugggagg ccgguccgag      540

agaacaauuc ucagcgugca gacccuggaa gaacuuaacg aucuguucgc cgaaaaugag      600

ggcacggaag aggucgagcu caacguggcg ucugccgaac agaucaauga cauccugacc      660

gggggacacc uuaacgcuga cucccaaaag gagauuagca aucugcuccu gccuucuucc      720

uuucccagcu ucgaugacaa agccaaggaa aaacagguua agaagcugau caacaaugug      780

gcuacaaaca uuuccaaagc cuggcucggc uacaaggcgg auuuuucuac cauccugaau      840

cuugccaaag ucgacaagga ucagaaaaag auuuucgcuu uugcccugca gggggaggac      900

gaagaggaua aggugcaaga gcucgaaagc cugcuugagc agucccagac agacaucgug      960

gaucgccuga ucgaaauucg gcacgcuauc guccucagcg agauuguucc ugugggaaug     1020

acccugucug aagccaugau cgacaaauau gaucagcaua aggaggaccu gauuacgcuc     1080

aaagccguga ucagaaacac aaaggacaaa aagaaggcug cgaaacugca agccaucuac     1140

gaucuuuacg ucaagaaucg ccacgcugac cuggccaaag ccaugaagcu caccggcauu     1200

aaaaagcggu ccgagcugcu ugauccggag gagcugaaga aagggaucag cucucuccug     1260

gacgauagcc ccgaagcugu ggagauuaag cagagaaucg aggagaaaac auuccugccu     1320

cuccagcgcu ccaacaacaa uggaguuauu cccaaccaac ugcaucaggu ggagcuugac     1380

gaaaucauua agaaacaguc uaaguauuac ccuuuccugg ccgagaaaaa ucccgaugaa     1440

agcgaggagg cgcagaagaa ggcuccgacc aaacucgacg cccugcugac cuuucggguc     1500

ccuuauuacg ugggccccau gaucacaaag gaggagcaag aagcccagaa cggccacucc     1560

uucgcuugga ugguuagacg cgauccucag gaccacgagg ccaucacgcc cuggaauuuu     1620

gagaaaaagg uggauaaaau ggcuagcgcc acccaguuca uuaagcggau gacaaccaag     1680

gacacauauc uucucgggga agauguccug cccgcgucuu cccugaaaua ccaacucuuu     1740

accgugcuga acgagcuuaa uaaccugaga gucaauggaa agaaacucac gagcgacgaa     1800

aaggaacagg ugaucgaagg ccuguucaaa aagcagaaga caguuaaagc cgacaaguuu     1860

gugaaauauu ggcaggcuaa gcauauuggg gccgauauca aagucaaggg acugucugac     1920

ccuuccaagu ucaacagcac cauguccacc uacauugauu uuaaaaagau cuucggcgac     1980

caacuuaaug augugaaccg ccagaaugac uuugagaaaa ucauugaaua cucuacaauc     2040

uucgaggauc ggaagauucu cgaggacaaa cugagaguug auuuugcuug gcugagcgag     2100

gaacagauca agcagcucug ccggcugcgc cuucaagggu ggggaagacu guccgacaaa     2160

cuccugaccc ggcugacaga ugccgacggc cagaacgugc uugaaagacu cuggaacucu     2220

aaugauaacu ucguccagau ugugagcgac ccgaauauca aggcgaagau cgagcaagag     2280

aaccagaaac ugcugcgcga cucccagagc gauaaugccg uggaaucuau ucucgacgau     2340

gcuuauacga gcccccagaa caagaaagcc auccggcaag uuaugagagu cgugcacgac     2400

auugugaagg cuugugaugg caaugucccu gccaaauucg ccaucgaguu ugcucgcucc     2460

caggaagacg aucccaagag aaccacagcg cgggugaacc agaugcagaa gauuuacgac     2520

caaaucucug augagguugu gucccagggg gucaaagaac agcugagcgg aaugaagucu     2580

cuuaaagacc gcuauuaccu guauuucaug cagggcggga gagaugccua caccggagcu     2640

aagcucaaua ucgaccggcu gagcgacuau gauguggacc auauuaugcc ucaauccuuu     2700

guuaaagaug acagccugga uaaccgcgug cuugucgcca gagccgugaa uaaccagaag     2760

ucugacaagg uucccgcucu ccuguucggc aauaaagugg ucgccgaucu ggggaucaca     2820

gugcgggaga ugugggacaa guggcagaaa cucggcauga uuuccaagcg caaacugagc     2880

aaccuucuga ccgauccgga cgcgcucacg gaguacagag cucaaggauu uauccggcgc     2940

cagcuggugg aaaccuccca ggucauuaag cugacagcca ccauccuuca gucugaguuc     3000

ccugauagca agauuaucga aguucccgcc aaauacaauu ccaucgugag aaagcaauuu     3060

gaccuguaua aaucucggga ggugaacgau uuccaccaug cuauugacgc cuaccucagc     3120

acaaucgucg gcaauuaucu guaccaggug uauccuaacc ucagacgcau guuuguuuac     3180

ggggaguuca agaaauuuuc cucuaacgcg gaagagagcg cucacgacgu ggcccggaga     3240

gucaagucca ugaauuuccu ggaugaccuu cugcgcggaa cccacggcga uaacauuuau     3300

ugccggagca cgggggaaau cguguuuaau agaaacgaca uuaucucuaa gcucaaacag     3360

gccuacuccu ucaagcagau gcugguuaca caagaggugu ucaccaaaaa gagcgcucug     3420

uuuaaucaga cagucuaccc cucuccuaaa cgcgauggau ccaagcggag cggccuuauc     3480

cccagaaaac gcgggaugga cccggaaauu uauggcggau acgcccauaa ccaggauucu     3540

cacuucgugc uggcuaagau cgagaagguu aaaggcuaua cccuccagau uguggggguc     3600

ccuauccggu cccuggaaau uaucaauaag gccgaggacu uuagcaccgc gcuccuggau     3660

aucauuaaau ccaaggugau guacaacgag aaaacaggac aacccaagag aggcaucaag     3720

aauguggaaa uucuugucaa caaacugccc uaucggcagc cuauccucga gaaggacguu     3780

agcuacaugc uggggucuag cgccuauaaa uacaauauua agcagaucac gcugucccgc     3840

cagucuauga aauacauucu ugauuauauc gacgauccga acuucaccaa gcaugaaaug     3900

acaaugaucg acgagcaaaa ugagagagaa ugucugcuca gcguguacga cgagauucug     3960

gaaaaggugg auaaauaucu cccccuguuu gacggaaaca aguuccggga gaaacuucac     4020

uccgcucgca aggaauuugu cucucugagc guggaugacg aggccuccgc uaucgagaaa     4080

auucugagag gccuccacgc caauucugaa augccuagcc uggagaaccu uaagaucaag     4140

cccgcccugg gguucuuuca gaugaaaaac ggcauuaccc ucuccaauga ugcuguucug     4200

cucuaccaga gcccuacagg acuguucgaa cggagaguga aggucucuga ccuucugaaa     4260

uga                                                                   4263


<210>  4481
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4481
auggccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguug ugaccgauga cuacagagug cccgccuuua aguucaaagu ccucggcaac      120

acagagaagg aaaagaucaa gaagaaucug auugggagca cgaccuuugu gccugcgcag      180

uccgcucaag gaacacgggu uuuccgcgug aacagacggc gcaucgauag acggaaucac      240

cgcauugccu aucuuagaga caucuuucag aaagagaucg aaaaggucga uaaaaacuuc      300

uaccggcgcc uggacgaguc uuuuagagug cucggcgaua agagcgagga gcugcagauu      360

aaacagccgu ucuuugggga caaggagcuu gaaaccgccu aucauaagaa auaccccaca      420

aucuaucauc ugcggaagca ucucgcugau gccgacaaaa auuccccugu ugcugauauu      480

cgcgaggugu acauggccau cucucacauu cugaaguaca gaggacauuu ccugacccuc      540

gaccggauca accccaauaa cauuaauaug caaaacagcu ggaucgauuu uaucgaaucc      600

ugccaggagg ucuucgaccu ggagauuagc gacgaaucua aaacaaucgu ggcgauuuuu      660

aaguccagcg auaaucgcca ggagaaagug aagaagaucc uuucuuauuu ccagcaagaa      720

cugcucaaaa aggacaaauc cauuuucaag cagcugcuuc agcugcucuu uggccugaaa      780

accaaguuca aggauuguuu ugagcuggaa gaggagccug accucaacuu cagcaaagaa      840

aauuacgaug agaaccugga aaacuuucuu ggcucucugg aggaagacuu cuccgauguc      900

uuugccaagc ucaaaguucu gagagacacg auccuucuga gcgggaugcu caccuauaca      960

ggaaccacac gggcuagauu cuccgccacc augguggagc gcuacgagga acaccggaag     1020

gaucugcaga gauuuaaauc uuucgugaag caaaaucuga gcgagcagga cuaucucgau     1080

aucuuuggcc gcaagacgcc caacggguuc gacgucgaca aagaaacaaa gggauacgug     1140

ggcuauauuu ccaauaaaau gguucugacc aacccucaga agcaaaagac aauccagcag     1200

aauuuuuacg auuacauuag cgggaagauc accggaauug agggcgcuga auauuuccuu     1260

aacaagaucu cugacggcac cuuccugcgg aaacucagaa caacggauaa uggggccgug     1320

ccgaaccaga uccacgcgua cgagcuggag aagauuaucg accgccaagg agaagauuau     1380

cccuuucuuc uggagaauaa agacaagcuc cuguccauuc ugaccuucaa aaucccuuac     1440

uaugucggcc cccucagcaa ggggaacaau ucucgguuug gauggauuaa aagaacaauc     1500

agccaggaua uucuggacga uaacgacgaa gauacccgca auggcaagau ccggccuugg     1560

aacuaccaga aacuuaucaa uauggacgag acaagagaug ccuucauuac caaccugauc     1620

gggaacgaca uuauccuccu gaaugagaaa gugcugccca agcgcucccu uauuuaugaa     1680

gagguuaugc uccagaacga acugacgcgg gugaaauaca aggacaaaua cggaaagaca     1740

cacuuuuucg auucugagcu gagacaagaa aucaucaaua accucuuuaa gaccaauaac     1800

aaacggguca gcuccgcuau gcugcuugac uaucuggaga auuacaccaa ccuccaggcc     1860

guggagauug ucagcggcau cgaaaagggc aaaucucuca auuccacacu gaagaccuau     1920

aacgaucuua aaacaauuuu cagcgaggaa cuccuggacu cugagaucua ccagaaggaa     1980

cuggaggaga uugugaaggu uaucacggug uuugaugaca aaaaguccau caaaaauuau     2040

cucauuaagu acuucgggca ccuggaauuu cuuaacgagg aacgcaucaa ucagcugagc     2100

aaacucagau auaccggaug gggccgguac uccgcuaagc ugcugcuuga uauuagagac     2160

gaggauacag gguucaaccu caugcaauuu cugcgcaaug acgaugaaaa ccggaaccug     2220

ucucagcuca ucagcgacuc cacccugucu uucgauagcg ccauuaaguc cauccagagc     2280

aaaucuacag ucgaggacga cguguucgau gagaucaaga aacuugccgg auccccggcu     2340

auuaagagag gcauccugaa uagcauuaaa aucguugacg aacucgugca gauuaucggg     2400

uacccucccc aaaacauugu caucgagaug gcgcgcgaaa auaugaccac ggaggagggc     2460

cagaagaaag ccaagaccag aaagacaaaa cuggaaucug cucugaagaa caucgaaaau     2520

uccgugcuug aaaacggaaa agugccucau agcgaugagc agcuccaauc ugaaaagcug     2580

uaucuguacu aucuccagaa uggcaaagac auguacagcc uggauaagac cggguccccc     2640

acaccccuuu aucgggacca gcuggaucag uacgagguug accacauuau cccuuauagc     2700

uuucucccga uugauucuau cgacaacaag guccugaccc accgcgagaa uaaccaaaga     2760

aaacugaaua acauucccga uaaggaaacg guggccaaua ugaaaccuuu cugggagaaa     2820

cuuuacaacg ccaaacugau cucccagaca aaguaccagc ggcucaccac aagcgaacgc     2880

acccccgacg gagugcugac cgaguccaug aaggcuggcu uuaucgaaag acagcucguc     2940

gagacacggc aaauuaucaa acauguggcc cgcauucugg acaauagauu ccuugauacg     3000

aagaucauug cgcugaaauc ucagcucauc accaacuuuc ggaacacauu ccacaucgcu     3060

aagauucgcg agcugaauga cuaucaucac gcccaugaug ccuaccuggc uguugugguc     3120

gggcagaccc uucugaaagc cuauccuaag cucgcucccg aacugaucua cggacaccac     3180

gcccauuuua acagacacga ggaaaauaag gcgacaauuc ggaaacaucu cuauagcaac     3240

aucaugagau ucuuuaauaa cccggacucc aaggugucua aagauauuug ggacugcaau     3300

cgcgaucugc cuaucauuaa ggagguuauc uacaacagcc aggugaauuu cgucaaacgg     3360

accaugauca agaagggcgc cuuuuauaac caaaaucccg uggggaaauu caacaagcag     3420

auugcugcca auaacagaua cccucuuaaa acgaagguuu ccucucugga cacaagcauc     3480

uacggcggau auggccccau gaacuccgcu cucagcauua ucauuaucgc cgaacgcuuc     3540

aaugagaaaa aggggaaaau cgagaccgug aaggaauuuc acgauauuuu caucauugac     3600

uacaagaaau uuaauaacaa cccuuuccag uuucugaaug auaccucuga gaacggauuc     3660

cugaagaaaa auaacaucaa ucggguccuu gcguuuuaug gcgugcccaa guacucccug     3720

augcagaaaa acgacgggac aagaaugcug uucgaaagca agucuaaucu gcacaaggcc     3780

acccaauuua aacucacaaa gacgcagaac gagcuguucu uucauaugcg ccggcuucug     3840

accaaaucca aucugaugga ucucaagagc gagucugcua uuaaagaauc ccagaacuuc     3900

auccugaagc acaaggagga auuugacaau aucagcaacc aacuuuccgc cuucagccag     3960

aaaaugcugg gagacacaac cucucucaga aaucugauua agggcuauaa cgagcgcaaa     4020

aucaaggaaa uugauagcuc cgaggaggug aucaaauacu ucuaugacaa uuuuauuaag     4080

auguucucuu uugucaagag cggggcuccg aaaggcauca acgauuucuu uaacaauaag     4140

uacacaguug cccggaugag accuaaaccc gacaagaaau uccucaacgc cacccugauu     4200

caucagucca ucacgggacu uuacgaaacc cggaucgauc ugucuaagcu gggcgaggac     4260

uga                                                                   4263


<210>  4482
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4482
auggccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguug ugaccgauga cuacagagug cccgccuuua aguucaaagu ccucggcaac      120

acagagaagg aaaagaucaa gaagaaucug auugggagca cgaccuuugu gccugcgcag      180

uccgcucaag gaacacgggu uuuccgcgug aacagacggc gcaucgauag acggaaucac      240

cgcauugccu aucuuagaga caucuuucag aaagagaucg aaaaggucga uaaaaacuuc      300

uaccggcgcc uggacgaguc uuuuagagug cucggcgaua agagcgagga gcugcagauu      360

aaacagccgu ucuuugggga caaggagcuu gaaaccgccu aucauaagaa auaccccaca      420

aucuaucauc ugcggaagca ucucgcugau gccgacaaaa auuccccugu ugcugauauu      480

cgcgaggugu acauggccau cucucacauu cugaaguaca gaggacauuu ccugacccuc      540

gaccggauca accccaauaa cauuaauaug caaaacagcu ggaucgauuu uaucgaaucc      600

ugccaggagg ucuucgaccu ggagauuagc gacgaaucua aaacaaucgu ggcgauuuuu      660

aaguccagcg auaaucgcca ggagaaagug aagaagaucc uuucuuauuu ccagcaagaa      720

cugcucaaaa aggacaaauc cauuuucaag cagcugcuuc agcugcucuu uggccugaaa      780

accaaguuca aggauuguuu ugagcuggaa gaggagccug accucaacuu cagcaaagaa      840

aauuacgaug agaaccugga aaacuuucuu ggcucucugg aggaagacuu cuccgauguc      900

uuugccaagc ucaaaguucu gagagacacg auccuucuga gcgggaugcu caccuauaca      960

ggaaccacac gggcuagauu cuccgccacc augguggagc gcuacgagga acaccggaag     1020

gaucugcaga gauuuaaauc uuucgugaag caaaaucuga gcgagcagga cuaucucgau     1080

aucuuuggcc gcaagacgcc caacggguuc gacgucgaca aagaaacaaa gggauacgug     1140

ggcuauauuu ccaauaaaau gguucugacc aacccucaga agcaaaagac aauccagcag     1200

aauuuuuacg auuacauuag cgggaagauc accggaauug agggcgcuga auauuuccuu     1260

aacaagaucu cugacggcac cuuccugcgg aaacucagaa caacggauaa uggggccgug     1320

ccgaaccaga uccacgcgua cgagcuggag aagauuaucg accgccaagg agaagauuau     1380

cccuuucuuc uggagaauaa agacaagcuc cuguccauuc ugaccuucaa aaucccuuac     1440

uaugucggcc cccucagcaa ggggaacaau ucucgguuug gauggauuaa aagaacaauc     1500

agccaggaua uucuggacga uaacgacgaa gauacccgca auggcaagau ccggccuugg     1560

aacuaccaga aacuuaucaa uauggacgag acaagagaug ccuucauuac caaccugauc     1620

gggaacgaca uuauccuccu gaaugagaaa gugcugccca agcgcucccu uauuuaugaa     1680

gagguuaugc uccagaacga acugacgcgg gugaaauaca aggacaaaua cggaaagaca     1740

cacuuuuucg auucugagcu gagacaagaa aucaucaaua accucuuuaa gaccaauaac     1800

aaacggguca gcuccgcuau gcugcuugac uaucuggaga auuacaccaa ccuccaggcc     1860

guggagauug ucagcggcau cgaaaagggc aaaucucuca auuccacacu gaagaccuau     1920

aacgaucuua aaacaauuuu cagcgaggaa cuccuggacu cugagaucua ccagaaggaa     1980

cuggaggaga uugugaaggu uaucacggug uuugaugaca aaaaguccau caaaaauuau     2040

cucauuaagu acuucgggca ccuggaauuu cuuaacgagg aacgcaucaa ucagcugagc     2100

aaacucagau auaccggaug gggccgguac uccgcuaagc ugcugcuuga uauuagagac     2160

gaggauacag gguucaaccu caugcaauuu cugcgcaaug acgaugaaaa ccggaaccug     2220

ucucagcuca ucagcgacuc cacccugucu uucgauagcg ccauuaagac aauccagucc     2280

aaaagcaccg ucgaggacga cguguucgau gagaucaaga aacuugccgg aucuccggcu     2340

auuaagagag gcauccucaa uuccauuaaa aucguugacg aacuggugca gauuaucggg     2400

uacccucccc aaaacauugu caucgagaug gcgcgcgaaa auaugacgac cgaggagggc     2460

cagaagaaag ccaagacaag aaagaccaaa cuggaaagcg cucugaagaa caucgaaaau     2520

ucugugcuug aaaacggaaa agugccucau uccgaugagc agcuccaaag cgaaaagcug     2580

uaucuguacu aucuccagaa uggcaaagac auguacucuc uggauaagac agggagcccc     2640

acgccccuuu aucgggacca gcuggaucag uacgagguug accacauuau cccuuauucc     2700

uuucucccga uugauagcau cgacaacaag guccugaccc accgcgagaa uaaccaaaga     2760

aaacugaaua acauucccga uaaggaaaca guggccaaua ugaaaccuuu cugggagaaa     2820

cuuuacaacg ccaaacugau cucucagacc aaguaccagc ggcucacaac cuccgaacgc     2880

acccccgacg gagugcugac agagagcaug aaggcuggcu uuaucgaaag acagcucguc     2940

gagacgcggc aaauuaucaa acauguggcc cgcauucugg acaauagauu ccuugauacc     3000

aagaucauug cgcugaaauc ccagcucauc acaaacuuuc ggaacaccuu ccacaucgcu     3060

aagauucgcg agcugaauga cuaucaucac gcccaugaug ccuaccuggc uguugugguc     3120

gggcagacac uucugaaagc cuauccuaag cucgcucccg aacugaucua cggacaccac     3180

gcccauuuua acagacacga ggaaaauaag gcgaccauuc ggaaacaucu cuauucuaac     3240

aucaugagau ucuuuaauaa cccggacagc aaggugucca aagauauuug ggacugcaau     3300

cgcgaucugc cuaucauuaa ggagguuauc uacaacucuc aggugaauuu cgucaaacgg     3360

acgaugauca agaagggcgc cuuuuauaac caaaaucccg uggggaaauu caacaagcag     3420

auugcugcca auaacagaua cccucuuaaa acaaagguua gcucccugga caccucuauc     3480

uacggcggau auggccccau gaauagcgcu cucuccauua ucauuaucgc cgaacgcuuc     3540

aacgagaaaa aggggaaaau cgagaccgug aaggaauuuc acgauauuuu caucauugac     3600

uacaagaaau uuaauaacaa cccuuuccag uuucugaaug auacaagcga gaacggauuc     3660

cugaagaaaa auaacaucaa ucggguccuu gcguuuuaug gcgugcccaa guacucucug     3720

augcagaaaa acgacgggac cagaaugcug uucgaaucca agagcaaucu gcacaaggcc     3780

acacaauuua aacucacgaa gacccagaac gagcuguucu uucauaugcg ccggcuucug     3840

acaaaaucua aucugaugga ucucaagucc gagagcgcua uuaaagaauc ucagaacuuc     3900

auccugaagc acaaggagga auuugacaau aucuccaacc aacuuagcgc cuucucccag     3960

aaaaugcugg gagacaccac aagccucaga aaucugauua agggcuauaa cgagcgcaaa     4020

aucaaggaaa uugauucuag cgaggaggug aucaaauacu ucuaugacaa uuuuauuaag     4080

auguucuccu uugucaaguc uggggcuccg aaagauauca acgacuucuu uaacaauaag     4140

uacaccguug cccggaugag accuaaaccc gauaagaaau uccucaacgc cacgcugauu     4200

caucagagca ucaccggccu uuacgaaaca cggaucgacc uguccaagcu gggagaggau     4260

uga                                                                   4263


<210>  4483
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4483
auggccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguug ugaccgauga cuacagagug cccgccuuua aguucaaagu ccucggcaac      120

acagagaagg aaaagaucaa gaagaaucug auugggagca cgaccuuugu gccugcgcag      180

uccgcucaag gaacacgggu uuuccgcgug aacagacggc gcaucgauag acggaaucac      240

cgcauugccu aucuuagaga caucuuucag aaagagaucg aaaaggucga uaaaaacuuc      300

uaccggcgcc uggacgaguc uuuuagagug cucggcgaua agagcgagga gcugcagauu      360

aaacagccgu ucuuugggga caaggagcuu gaaaccgccu aucauaagaa auaccccaca      420

aucuaucauc ugcggaagca ucucgcugau gccgacaaaa auuccccugu ugcugauauu      480

cgcgaggugu acauggccau cucucacauu cugaaguaca gaggacauuu ccugacccuc      540

gaccggauca accccaauaa cauuaauaug caaaacagcu ggaucgauuu uaucgaaucc      600

ugccaggagg ucuucgaccu ggagauuagc gacgaaucua aaacaaucgu ggcgauuuuu      660

aaguccagcg auaaucgcca ggagaaagug aagaagaucc uuucuuauuu ccagcaagaa      720

cugcucaaaa aggacaaauc cauuuucaag cagcugcuuc agcugcucuu uggccugaaa      780

accaaguuca aggauuguuu ugagcuggaa gaggagccug accucaacuu cagcaaagaa      840

aauuacgaug agaaccugga aaacuuucuu ggcucucugg aggaagacuu cuccgauguc      900

uuugccaagc ucaaaguucu gagagacacg auccuucuga gcgggaugcu caccuauaca      960

ggaaccacac gggcuagauu cuccgccacc augguggagc gcuacgagga acaccggaag     1020

gaucugcaga gauuuaaauc uuucgugaag caaaaucuga gcgagcagga cuaucucgau     1080

aucuuuggcc gcaagacgcc caacggguuc gacgucgaca aagaaacaaa gggauacgug     1140

ggcuauauuu ccaauaaaau gguucugacc aacccucaga agcaaaagac aauccagcag     1200

aauuuuuacg auuacauuag cgggaagauc accggaauug agggcgcuga auauuuccuu     1260

aacaagaucu cugacggcac cuuccugcgg aaacucagaa caacggauaa uggggccgug     1320

ccgaaccaga uccacgcgua cgagcuggag aagauuaucg accgccaagg agaagauuau     1380

cccuuucuuc uggagaauaa agacaagcuc cuguccauuc ugaccuucaa aaucccuuac     1440

uaugucggcc cccucagcaa ggggaacaau ucucgguuug gauggauuaa aagaacaauc     1500

agccaggaua uucuggacga uaacgacgaa gauacccgca auggcaagau ccggccuugg     1560

aacuaccaga aacuuaucaa uauggacgag acaagagaug ccuucauuac caaccugauc     1620

gggaacgaca uuauccuccu gaaugagaaa gugcugccca agcgcucccu uauuuaugaa     1680

gagguuaugc uccagaacga acugacgcgg gugaaauaca aggacaaaua cggaaagaca     1740

cacuuuuucg auucugagcu gagacaagaa aucaucaaua accucuuuaa gaccaauaac     1800

aaacggguca gcuccgcuau gcugcuugac uaucuggaga auuacaccaa ccuccaggcc     1860

guggagauug ucagcggcau cgaaaagggc aaaucucuca auuccacacu gaagaccuau     1920

aacgaucuua aaacaauuuu cagcgaggaa cuccuggacu cugagaucua ccagaaggaa     1980

cuggaggaga uugugaaggu uaucacggug uuugaugaca aaaaguccau caaaaauuau     2040

cucauuaagu acuucgggca ccuggaauuu cuuaacgagg aacgcaucaa ucagcugagc     2100

aaacucagau auaccggaug gggccgguac uccgcuaagc ugcugcuuga uauuagagac     2160

gaggauacag gguucaaccu caugcaauuu cugcgcaaug acgaugaaaa ccggaaccug     2220

ucucagcuca ucagcgacuc cacccugucu uucgauagcg ccauuaaguc cauccagagc     2280

aaaucuacag ucgaggacga cguguucgau gagaucaaga aacuugccgg auccccggcu     2340

auuaagagag gcauccugaa uagcauuaaa aucguugacg aacucgugca gauuaucggg     2400

uacccucccc aaaacauugu caucgagaug gcgcgcgaaa auaugaccac ggaggagggc     2460

cagaagaaag ccaagaccag aaagacaaaa cuggaaucug cucugaagaa caucgaaaau     2520

uccgugcuug aaaacggaaa agugccucau agcgaugagc agcuccaauc ugaaaagcug     2580

uaucuguacu aucuccagaa uggcaaagac auguacagcc uggauaagac cggguccccc     2640

acaccccuuu aucgggacca gcuggaucag uacgagguug accacauuau cccuuauagc     2700

uuucucccga uugauucuau cgacaacaag guccugaccc accgcgagaa uaaccaaaga     2760

aaacugaaua acauucccga uaaggaaacg guggccaaua ugaaaccuuu cugggagaaa     2820

cuuuacaacg ccaaacugau cucccagaca aaguaccagc ggcucaccac aagcgaacgc     2880

acccccgacg gagugcugac cgaguccaug aaggcuggcu uuaucgaaag acagcucguc     2940

gagacacggc aaauuaucaa acauguggcc cgcauucugg acaauagauu ccuugauacg     3000

aagaucauug cgcugaaauc ucagcucauc accaacuuuc ggaacacauu ccacaucgcu     3060

aagauucgcg agcugaauga cuaucaucac gcccaugaug ccuaccuggc uguugugguc     3120

gggcagaccc uucugaaagc cuauccuaag cucgcucccg aacugaucua cggacaccac     3180

gcccauuuua acagacacga ggaaaauaag gcgacaauuc ggaaacaucu cuauagcaac     3240

aucaugagau ucuuuaauaa cccggacucc aaggugucua aagauauuug ggacugcaau     3300

cgcgaucugc cuaucauuaa ggagguuauc uacaacagcc aggugaauuu cgucaaacgg     3360

accaugauca agaagggcgc cuuuuauaac caaaaucccg uggggaaauu caacaagcag     3420

auugcugcca auaacagaua cccucuuaaa acgaagguuu ccucucugga cacaagcauc     3480

uacggcggau auggccccau gaacuccgcu cucagcauua ucauuaucgc cgaacgcuuc     3540

aaugagaaaa aggggaaaau cgagaccgug aaggaauuuc acgauauuuu caucauugac     3600

uacaagaaau uuaauaacaa cccuuuccag uuucugaaug auaccucuga gaacggauuc     3660

cugaagaaaa auaacaucaa ucggguccuu gcguuuuaug gcgugcccaa guacucccug     3720

augcagaaaa acgacgggac aagaaugcug uucgaaagca agucuaaucu gcacaaggcc     3780

acccaauuua aacucacaaa gacgcagaac gagcuguucu uucauaugcg ccggcuucug     3840

accaaaucca aucugaugga ucucaagagc gagucugcua uuaaagaauc ccagaacuuc     3900

auccugaagc acaaggagga auuugacaau aucagcaacc aacuuuccgc cuucagccag     3960

aaaaugcugg gagacacaac cucucucaga aaucugauua agggcuauaa cgagcgcaaa     4020

aucaaggaaa uugauagcuc cgaggaggug aucaaauacu ucuaugacaa uuuuauuaag     4080

auguucucuu uugucaagag cggggcuccg aaagauauca acgacuucuu uaacaauaag     4140

uacacaguug cccggaugag accuaaaccc gauaagaaau uccucaacgc cacccugauu     4200

caucagucca ucacgggccu uuacgaaacc cggaucgacc ugucuaagcu gggagaggau     4260

uga                                                                   4263


<210>  4484
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4484
auggccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugcuguug ugaccgagga uuacagagug cccgccuuua aguucaaagu ccucggcaac      120

acagaaaagg agaaaaucaa gaagaaucug auugggagca cgaccuuugu gccugcgcag      180

uccgcucaag gaacacgggu uuuccgcgug aacagacggc gcaucgacag acggaaucac      240

cgcauugccu aucuuagaga uaucuuucag aaagaaaucg agaagauuga caaaaacuuc      300

uaccggcgcc uggaugaauc uuuuagaguc cucggcgaca agagcgagga uauccagauu      360

aaacagccgu ucuuugggaa uaaggagcug gaaaccgccu aucauaagaa auaccccaca      420

aucuaucacc uucggaagca ucuggcugac gccgauaaaa acuccccugu ggcugacauu      480

cgcgagguuu acauggugcu cucucacauc cugaaguaca gaggacauuu ccugacccuc      540

ggcaaaauug aucccaauaa caucaauaug caaaacagcu ggaucgacuu uauugaaucc      600

ugccaggaug ccuucgaccu ggagaucagc gacgagucua agacaauugc ggccaucuuu      660

aaauccagcg aaaaucggca ggagaagguc aagcagauuc uuucuuauuu ccaacaggaa      720

cugcucaaaa aggauaaauc caucuucaag cagcugcuuc agcugcucuu uggccugaaa      780

accaaguuca aggacuguuu ugagcuggaa gaggagccug aucucaacuu cagcaaagaa      840

aauuacgacg agaaccugga aaaccuucug ggggcucucg aggaagauuu uucugacgug      900

uucgccaagc ugaaaauccu ucgcgauacg auucugcucu ccagcaugcu gaccuauaca      960

ggagcuaccc acgccagauu uuccgcgaca augguggagc gguacgagga acacagaaag     1020

gaccugcaac gcuucaaauc uuuugucaag cagaaucuca gcgagcagga uuaucuggac     1080

aucuucggcc ggaagacccc cacgggguuu gauguugaca aagaaacaaa gggauacgug     1140

ggcuauauuu ccaacaaaau ggugcuuacc aauccucaaa agcagaaaac aauccagcag     1200

aacuucuacg acuacauuag cgggaagauc accggaaucg agggcgccga augcuuucug     1260

aauaagauuu cugauggcac cuuccucaga aaacugcgca caacggacaa cggggcuauc     1320

ccgaaucaaa uucaugccua ugagcuugag aagaucauug aacggcaggg aagagauuac     1380

cccuuccugc ucgagaacaa agacaagcug cuguccaucc ucaccuuuaa aauuccuuau     1440

uacgucggcc cccuggcuaa ggggaauaac agccgcuucg ccuggaucaa acggacaacc     1500

ucucaggaug ugcuugacga uaaugacgaa gauacaagaa acggaaagau ccgcccuugg     1560

aauuaucaca agcugauuaa cauggacgag acccgggaug ccuuuaucau uaaucucauc     1620

ggcaacgaca uuauccugcu gaacgagaaa guucuuccca agagaagccu caucuacgaa     1680

gaggugaugc ugcagaauga acugacgcgc gucaaauaua aggauaaaua cgggaagauu     1740

cauuucuuua acuccgagcu ccggcaagaa aucauuaaua accuguucaa gacaaauucu     1800

aaaagaguga gcuccgcuau gcuucugagc uaccucgaga acuuuaccaa ucugcaggcg     1860

guugagaucg ugucuggaau ugaaaagggc aaaucccuga acagcacccu uaagacauau     1920

aaugaccuca aaaccaucuu cucugaggaa cugcuggacu ccgagaucua ccagaaggaa     1980

cucgaggaga uuaucaaggu cauuacagug uuugaugaca aaaagagcau caaaaacuau     2040

cugacgaagu acuucggcca ccuugaauuu cugaaugagg agaaaauuaa ccagcucucc     2100

aagcugcggu auaccgggug gggacgcuac ucuggcaagc ugcuucucgg gaucagagau     2160

gaagacacag gauucaaucu gaugcaauuu cugcggaacg augacgaaaa uagaaaccuc     2220

acccagcuga ucagcgauac aacccuuucc uucgacucug ccauuaaagc uauccagagc     2280

aaguccacgg ucgaggauga cguguucgag gaaauuaaaa accuggccgg cagcccggcc     2340

aucaagcgcg ggauucucaa uucuaucaaa auuguugaug agcuggugca gaucaucggc     2400

uauccucccc aaaacauuau cauugaaaug gcucgggaga auaugaccac agaggaagga     2460

cagaagaaag ccaagaccag aaaguccaaa cuggagagcg cgcuuaagaa caucgaagac     2520

ucuguccucg aguccggcaa agugccucac agcgaugaac agcugcaauc ugagaagcug     2580

uaccucuacu aucugcagaa ugggaaagac auguacagcc uugacaagac aggauccccc     2640

acgccccugu aucgcgauca gcucgaccag uacgagguug aucauauuau cccuuauagc     2700

uuucugccga ucgacucuau ugauaacaag gugcugaccc acagagaaaa uaaccaacgg     2760

aaacuuaaua acauccccga caaggagaca gucgcuaaua ugaaaccuuu cugggaaaag     2820

cuguacaacg ccaaacucau uucccagacc aaguaucagc gccugacaac cagcgagaga     2880

acccccgaug gcgugcucac agaauccaug aaggccgggu uuaucgagcg gcagcuggug     2940

gagacgcgcc aaauuaucaa acauguugcu agaauccuug acaaucgguu cucugauacc     3000

aagauuauca cacugaaaag ccagcucguc accaacuuuc gcaauacauu ccacauugcc     3060

aagaucagag aacugaacga cuaccaucac gcucacgaug ccuaccuggc gguggugguc     3120

ggacagaccc uucugaaagc cuauccuaag cucgcucccg agcugauuua cggccaucac     3180

gcccauuuua accggcacga agagaauaag gcuacgcucc gcaaacaccu guauuccaac     3240

aucaugagau ucuuuaauaa cccggacucu aaggugagca aagacauuug ggauuguaau     3300

cgggaccuuc cuaucguuaa ggaugugauc uacaacuccc agauuaauuu cgucaaaaga     3360

acaaugauca agaagggggc cuuuuauaac caaaaucccg ugggcaaacu gaacaagcag     3420

auugcggcca auaaccgcua cccucucaaa accaagguuu cuagccugga caccuccauc     3480

uauggaggcu acgggcccau gaauagcgcu cugucuauua ucaucauugc cgaacgguuc     3540

aacgagaaaa agggaaaaau cgagacagug aaggaauuuc augauauuuu uaucauugac     3600

uacgagaagu ucaauaacaa uccuuuucag uuccuuaaca acgucuccga agagggcuuu     3660

cugaaaaaga augaucucaa cagagugcug gcuuucuauc gcaucagcaa auacucucuc     3720

augcaaaaga ucgacgggac ccggaugcug uuugagucca aaagcaaucu ucacaagguu     3780

acacaguuca agcugacgaa aacccagaac gaacuguuuu uccauaugaa gagacuccug     3840

acaaaaucua aucuuaugga ucugaagucc gagagcgcca uuaaagaauc ccagaacuuu     3900

auccaaaccc accagaauga guucgacaac auugccgauc gccucagcaa uuauucucag     3960

aagaugcugg gagaaacaaa gagccuccag acccugauca acggcuacaa ugagcggccc     4020

auuaaacaaa ucgacaacaa ugaggaaacg auuagauauu acuaugauaa cuuuaucaag     4080

auguucuccu ucgugaaauc uggggcuccg aaggacauca uuaauuucuu caacgacaaa     4140

uacaccgucc cucgcaugcg gcccaagccc gauagaaaga ugcuugacgc gacacugauc     4200

caucagagca uuaccggccu guacgagaca cggaucgauc ucuccaaacu gggagaagac     4260

uga                                                                   4263


<210>  4485
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4485
augagcagaa agaacuacgu ggacgauuau gccaucuccc uggacauugg caaugcuucu       60

gucgggugga gcgccuucac ccccaacuac cggcucguuc gcgcgaaagg acacgagcug      120

aucggcguga gacuuuuuga uccugcugac acagccgaau cccggcgcau ggccagaacg      180

acccggcgca gauauucucg gcgcagaugg cggcugcgcc uccuggaugc ucuuuucgac      240

caggcccuga gcgagauuga uccguccuuu cucgcuagac ggaaguacuc uugggugcau      300

cccgacgaug aaaauaacgc cgacugcugg uaugggagcg uccuguucga uuccaaugag      360

caagacaaac gcuuuuacga aaaguauccu acaaucuacc accugagaaa agcgcucaug      420

gaggaugaca gccagcauga cauucgggag aucuaccugg ccauccacca uauggugaag      480

uauagaggaa acuuccuugu ugaaggcacc cuggagucuu ccaaugcuuu uaaggaagau      540

gagcuccuga aacuucuggg gcgcauuaca cgguacgaga ugagcgaagg agagcagaac      600

ucugacaucg aacaggauga cgagaauaag cucguggccc ccgcuaacgg ccaacuggcc      660

gaugcgcugu gugccaccag aggcucccgc agcaugcggg ucgacaaugc ucucgaagcc      720

cugucugcug ugaacgaucu uuccagagag cagcgcgcca uuguuaaagc caucuucgcu      780

gggcuggagg gaaauaagcu cgaccuggcg aaaauuuuug ugagcaagga guucuccucu      840

gagaacaaga aaauccuugg cauuuauuuu aauaagagcg auuacgaaga gaaaugcguc      900

cagaucgugg acuccgggcu gcucgaugac gaagaacggg aguuccugga cagaaugcag      960

ggacaauaua acgccaucgc ucugaagcag cuccugggcc gcagcacauc uguguccgau     1020

agcaaaugug ccucuuacga cgcccaccgg gcuaacugga aucuuauuaa gcugcagcuc     1080

agaaccaaag aaaacgagaa ggauaucaau gaaaacuaug ggauucuggu cggauggaag     1140

aucgacagcg gccagcgcaa auccguucgg ggcgagucug ccuacgaaaa uaugagaaag     1200

aaagcgaacg uguuuuucaa gaaaaugauu gagacgagcg aucuuuccga gaccgacaag     1260

aaucggcuga uccacgauau cgaagaggac aaacucuucc cuauucaacg cgauagcgac     1320

aacgggguga ucccccauca gcugcaccag aaugaacuga agcaaauuau caagaaacag     1380

ggaaaguacu auccuuuucu ccuggaugcu uucgagaaag acggcaagca gauuaacaag     1440

aucgaggggc uucugacauu uagagucccg uacuucgugg gaccccucgu ugugccugaa     1500

gaucugcaga aaucugacaa uuccgagaac cauuggaugg uccggaagaa aaagggcgaa     1560

aucacccccu ggaauuuuga cgagauggug gauaaagacg ccagcgggag aaaguucauu     1620

gaacgccugg uuggaacaga uucuuaucuu cucggcgagc cuacccugcc caagaacucc     1680

cugcucuacc aagaguauga agugcugaau gagcuuaaca acguccggcu gagcgugaga     1740

acgggcaauc acuggaacga caaacgcaga augcggcucg ggcgcgaaga gaagacacug     1800

cugugccaga gacuuuuuau gaaaggacag accgucacaa agcggaccgc cgaaaaucuc     1860

cugcgcaaag aguacggcag aaccuaugag cuguccgggc ucucugauga aagcaaguuc     1920

acauccucuc ugagcacgua cggaaaaaug ugucggaucu uuggcgagaa guacgugaac     1980

gaacaccgcg accuuaugga gaagauuguu gaacugcaga ccguguucga ggauaaagag     2040

acacuccugc aucaacugag acagcuugaa gggaucuccg aggcugacug cgcccuccug     2100

gucaauaccc acuauacagg cuggggacgg cugagccgca agcuccugac cacgaaagcu     2160

ggcgaaugua agauuucuga ugacuuugcc ccgagaaaac auuccaucau ugagaucaug     2220

cgggcggagg auagaaaccu uauggaaauc auuacagaca agcagcuggg guucagcgau     2280

uggaucgagc aggaaaaucu cggagccgag aacgggucuu cccugaugga agugguugac     2340

gaccugcgcg ugagcccuaa ggucaaacgg ggcauuaucc aaucuauuag acuuaucgau     2400

gacaucagca aggcuguggg aaaacgcccc ucccggauuu uucucgagcu ggccgaugac     2460

auccagccua gcggcagaac cauuucucgc aagucccggc ugcaggaucu cuacagaaau     2520

gcuaaccugg ggaaagaguu caagggcauc gccgacgaac uuaaugcgug cagcgauaag     2580

gaccugcagg augaccgccu cuuccuguau uacacccaac ugggaaaaga uauguauaca     2640

ggcgaggaac uugaccugga ccggcucucc ucugccuacg auauugacca caucaucccc     2700

caggcuguga cccagaacga uagcauugac aauagaguuc uggucgcccg ggcugagaac     2760

gcccgcaaga cagauuccuu uacguauaug ccccaaaucg ccgacagaau gcggaauuuc     2820

uggcagauuc uccuggauaa cgggcuuauc ucucgcguga aauuugaaag acugacccgg     2880

cagaacgagu ucagcgagag agaaaaggag cgcuuugugc agcggucccu cgucgaaaca     2940

agacaaauua ugaaaaaugu ggcuacccug augcgccagc gguacggaaa cucugcggcc     3000

guuaucggcc ugaaugcuga gcuuacaaag gaaaugcaua gauaccuggg guucagccac     3060

aagaaccgcg acaucaauga uuaucaccau gcccaggacg cccucugugu gggaauugcu     3120

ggccaguuug ccgcgaaccg gggguucuuu gcugauggcg aggucuccga cggagcccaa     3180

aauagcuaca accaguaucu gagagauuac cuccgcggcu auagagagaa acugucugcc     3240

gaagaccgga agcaggggcg cgcuuucgga uuuaucgugg gcuccaugag aagccaggac     3300

gagcaaaaac ggguuaaucc ucgcaccggg gaaguggucu ggucugagga ggauaaggac     3360

uaccuuagaa aagugaugaa cuaucggaag augcugguua cgcagaaagu gggagaugac     3420

uucggcgccc ucuacgauga aaccagauac gcggcuacag acccgaaggg gauuaagggc     3480

auccccuuug auggagccaa acaggacacc ucccuguaug gcggguucag cucugccaag     3540

ccugcuuacg ccguccugau ugaguccaaa ggaaagacac gccuugugaa ugugaccaug     3600

caagaauaua gccugcucgg cgaucggccc uccgacgaug agcugagaaa aguccucgcu     3660

aagaagaaaa gcgaauacgc caaggcgaac auccugcuuc gccacguucc uaaaaugcag     3720

cugauucggu augggggagg ccugauggug aucaagucug ccggcgagcu caauaacgcu     3780

cagcagcugu ggcuucccua cgaggaauau ugcuacuucg acgaucugag ccaagggaaa     3840

ggaucccucg agaaggacga ccugaagaaa cuccuggauu cuauccuugg cagcgugcag     3900

ugucuguacc cguggcauag auuuacggaa gaggaacugg ccgaccucca cgucgcuuuc     3960

gauaagcugc cugaggacga gaaaaagaau gugauuacag ggaucguuuc cgcccuucac     4020

gccgaugcua aaaccgcgaa ccugucuauu gugggaauga caggcagcug gcggcgcaug     4080

aacaauaagu ccggguauac cuuuucugac gaagaugagu ucaucuuuca gagccccucc     4140

ggacucuucg aaaagagagu caccgugggc gagcugaaac ggaaggccaa aaaggaaguu     4200

aacagcaaau accgcacaaa ugagaagaga cucccuacgc ugucuggcgc uucccagccc     4260

uga                                                                   4263


<210>  4486
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4486
auggccaagc ccaaagacua caccaucggc cuggauauug ggacaaacag cgugggaugg       60

gucguuacgg acgaucagaa uaacauccuc agaauuaagg gcaaaaaggc uaucggggcc      120

cggcuguucg cggagggaaa aguggcugcc gaacgcagau ccuuucggac cacacgcaga      180

cggauuucuc gcagacggug gcgcaucaaa cuucuggagg aacucuucga cgaggaaauc      240

gccaaggugg auccuagcuu uuucgcuaga cugcacgagu ccuggauuuc uccgaaagac      300

aagcggaaac gcuauagcgc caucgucuuu cccuccccug aggaagauaa gaaauuccau      360

gagucuuacc ccaccauuua ucaccuuaga gacaagcuga ugaaggauga ccaaaaacau      420

gauauccggg aaauuuacau cgcugugcac cagaugauua agguucgcgg caauuuucuc      480

caugacgaga gcguggagac auauagaucc gggaugagcu cucugggagg ccgguccgaa      540

agaaccaucc ugagcgucca gacacucgag gaacugaacg aucuuuucgc cgagaaugaa      600

ggcaccgagg agguggaacu gaacguugcg ucugccgagc agaucaauga cauucucacg      660

ggggaccacc ugaacgcuga uucccaaaaa gaaaucagca aucuucugcu cccuucuucc      720

uuucccagcu ucgacgauaa ggccaaagag aaacagguga aaaagcugau uaacaauguc      780

gcuaccaaca ucuccaaggc cuggcuggga uacaaagcgg acuuuucuac aauucucaau      840

cuggccaagg uggauaaaga ccagaagaaa aucuucgcuu uugcccuuca gggcggggau      900

gaagaggaca aggugcaaga gcuggaaagc cuccuggagc agucccagac cgauaucguc      960

gaccgccuua uugaaauccg gcacgcuauu guucugagcg agaucgugcc ugugggaaug     1020

acacucucug aagccaugau ugauaaguau gaccagcaua aagaggaccu gaucacccug     1080

aaggccguca ucagaaacac gaaagauaag aaaaaggcug cgaagcucca agccauuuac     1140

gaccuguacg ugaauaaccg ccacgcugau cuugccaaag ccaugaagcu gacaggcauc     1200

aaaaagcggu ccgagcuccu ggacccggag gaacuuaaaa aggcuauuag cucucugcuc     1260

gaugacagcc ccgaagccgg ggagaucaag cagagacugg agggaaaaac cuuccugccu     1320

cuccagcgcu ccaacaauaa cggcguuauu cccaaucaac ugcaucaggu ggaacuugau     1380

gagaucauua agaaacaguc uaaguauuac ccuuuccugg cggaaaagaa ccccgacgag     1440

agcgaagagg cucagaagaa agccccgaca aagcucgaug cccugcugac cuuucggguc     1500

ccuuauuacg ugggccccau gaucaccaag gaggaacaag aggcucagga cgggcacucc     1560

uucgccugga ugguuagacg cgauccucag gaccacgaag cuaucacacc cuggaauuuu     1620

gagaaaaagg uggauaaaau ggccagcgcg acgcaguuca uuaagcggau gaccacaaaa     1680

gacaccuauc uucucggaga agacguccug cccgccucuu cccugaagua ccaacucuuu     1740

acagugcuga acgagcuuaa uaaccugaga gucaauggca agaaacucac cagcgaugag     1800

aaggaacagg ugaucgaggg gcuguucaaa aagcagaaaa cgguuaaggc ugacaaguuu     1860

gugaaauauu ggcaggccau ucauaucgga gcugauauua aggucaaagg ccugucugac     1920

ccuuccaagu ucaacagcac aauguccacc uacaucgauu uuaaaaagau cuucggggac     1980

caacuuaaug augccaaccg ccagaaugac uuugaaaaga uuaucgagua cucuaccauu     2040

uucgaagauc ggaaaauccu cgaggacaag cugagagcgg auuuugccug gcugagcgag     2100

gaacagauua aacagcucug ccggcugcgc cuucaaggau ggggcagacu guccgacaag     2160

cuccugacac ggcugaccga ugcugacggc cagaacgugc uugagagacu cuggaauagc     2220

aacgacaacu ucguucagau cgugagcgau ccgaauauca aagccaagau ugaacaagag     2280

aaccagaaac ugcugcgcga cgggcagucc gaugacgcug ucgagagcau ccucgaugac     2340

gccuauacau cuccccagaa uaagaaggcc auucggcaag ugaugagagu gguucacgau     2400

aucgucaagg cuugugacgg aaacgugccu gcgaaguucg ccauugaauu ugcucgcucc     2460

caggaggaug accccaaaag aacgaccgcc cgggugaauc agaugcagaa gaucuacgau     2520

caaaucagcg acgaagucgu gucucagggc guuaaagagc agcuguccgg gaugaaaagc     2580

cuuaaggacc gcuauuaccu guauuucaug cagggaggca gagaugccua cacaggggcu     2640

aagcucaaca uugaccggcu gucugauuau gacaucgauc auauuaugcc ucaaucuuuu     2700

gugaaggacg auucccugga caaucgcguc cuuguggcca gagcgguuaa caaucagaaa     2760

agcgauaagg ugcccgcuuu ccucuuuggc aacaaagucg uggccgaccu gggaaucacc     2820

guucgggaaa ugugggauaa guggcagaag cugggcauga uuucuaaacg caagcucucc     2880

aaucugcuua cagacccgga ugcccugacc gaguacagag cucaaggguu cauccggcgc     2940

cagcucgugg agacgagcca ggucauuaaa cugaccgcca caauccugca guccgaauuu     3000

ccugacucua agaucauuga ggugcccgcg aaauacaaca gcaucgugag aaagcaauuc     3060

gaccuuuaua agucccggga agucaaugau uuucaccaug cuauugacgc cuaccugucu     3120

accaucguug gaaacuaucu cuaccaggug uauccuaauc ugagacgcau guucguguac     3180

ggcgaguuua aaaaguucag cuccaacgcc gaagagucug cucacgauau ggcccggaga     3240

gucaaaagca ugaacuuucu cgacgaucug cuucgcggga cacacggaga caauauuuau     3300

ugccggucca ccggcgagau cguguucaac agaaaugaua ucauuagcaa gcugaaacag     3360

gcuuacucuu ucaagcagau gcucguuacg caagaagugu uuacacgcaa guccgcccug     3420

uucgaccaga ccgucuaccc cagcccugag cgggauucua aaaagagauc cgggcugauc     3480

ccccgcaaaa agggcaugga cacagaaauu uauggaggcu acagcgggaa caaagaugcg     3540

uauuuugugc uugccgaggc uguuaaggaa aaaggacaua cccugcagau cgugggcguc     3600

ccgauucggg cccucaauac ccugaagaac ucugcuaauu acuccgagaa gcuccuggag     3660

aucaucaaac cucaggugau guucaacaag aaaaauggga agcccaaaaa gggaguuaac     3720

gacgugcgga uucuuaugga uaauaucccc uauagacaac cuguccugga aggcggggcc     3780

cucuacaugc ugggcagcuc cacauauaag uacagccuga aacagauuac gcuuucucag     3840

gagagcauga aguacauccu ggacuauauu gacgauccgu ccuuuaacaa acacaucaug     3900

gugaauauug accaucagga ugaaaagaaa ugucuccugu cuguguacga cgagauccuc     3960

gauaagaugg acaaguaucu gccccuuuuc gauaucaacg gauuucgcaa aaagcugcac     4020

gacggcagag agaaauucgu caagcugccu guugaaaaac aagccgaggu ggugaaggau     4080

auucuccggg ggcugcacgc uaauagcgaa augaccaagc ccuccgaccu uggacugaca     4140

accgcgcucg gcuuuaugca ggccccugau gggaucaaaa uuucugacga cgcugucuuc     4200

aucuaccaga gccccacagg acuguuugag cgcagaguga agguuuccga ucuccugaaa     4260

uga                                                                   4263


<210>  4487
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4487
augaccaagu ucaacaaaaa uuacagcauc ggccuggaca uugggguguc cucugucgga       60

uaugccguug ugacagagga uuacagagug cccgcuuuua aguucaaagu ccucggcaac      120

acggaaaagg agaaaaucaa gaagaaucug auugggagca ccacauuugu guccgcccag      180

ccugcgaaag gaacccgggu uuuccgcgug aacagacggc gcaucgacag acggaaucac      240

cgcauuacau aucuuagaga uaucuuucaa aaggaaaucg agaaagucga caagaacuuc      300

uaccggcgcc uggaugaauc uuuuagagug cucggcgaca aaagcgagga ucugcagauu      360

aagcagccgu ucuuugggga caaggagcuu gaaaccgcuu aucauaaaaa guaccccaca      420

aucuaucauc ugcggaaaca ccucgccgau gccgacaaga auagcccugu ugcugauauu      480

cgcgaggugu acauggccau cucucacauu cugaaauaca gaggacauuu ccugacccuc      540

gacaagauca accccaauaa cauuaauaug cagaacagcu ggaucgauuu uaucgaaucc      600

ugccaagagg ucuucgaccu ggagauuagc gacgaaucua aaaauaucgc ugauauuuuu      660

aaguccagcg agaaccggca ggaaaaggug aaaaagaucc uuccuuauuu ccagcaggag      720

cugcucaaaa aggacaaauc uauuuucaag caacugcuuc agcugcucuu uggccugaag      780

acgaaauuca aggauuguuu ugaacuggag gaggaacccg accucaauuu cuccaaagag      840

aacuacgaug aaaaccugga gaauuuucuu ggcagccugg aagaggacuu cucugaugug      900

uuugccaagc ucaaaguccu gcgcgacacc auccuucugu ccgggaugcu cacauauacc      960

ggagcgacac acgccagauu cagcgcuacc augguugagc gguacgaaga acacagaaag     1020

gaucugcagc gcuuuaaguu cuuuaucaaa cagaaccugu ccgaacaaga cuaucucgau     1080

auuuucggcc ggaagacgca gaauggguuu gacguggaca aagagacaaa gggauacgug     1140

ggcuauauca ccaacaaaau gguccugaca aauccucaga agcaaaagac cauucagcag     1200

aacuucuacg auuacaucuc ugggaaaauu accggaaucg aaggcgccga guauuuccuu     1260

aauaagauca gcgacggcac auuucugaga aaacuccgca cguccgauaa cggggcuauu     1320

ccgaaucaga uccaugccua cgagcuggaa aagauuaucg agcggcaagg aaaagacuau     1380

cccuuccuuc uggaaaacaa ggauaaacuc cugagcauuc ugaccuuuaa gaucccuuac     1440

uaugugggcc cccucgcgaa ggggucuaau uccagauucg ccuggauuaa acgcgcuaca     1500

agcucugaca uccuggauga caacgaugag gacacccgga auggaaagau cagaccuugg     1560

aacuaccaga aacuuauuaa uauggaugag acacgcgacg ccuuuaucac caaccugauu     1620

ggcaacgaua ucauucuccu gaaugaaaag guucugccca aacggagccu uaucuaugag     1680

gaagugaugc uccagaacga gcugacgaga gucaaguaca aggacaaaua cgggaaggcu     1740

cacuucuuug acuccgaacu gcgccagaau aucauuaacg gacucuucaa aaauaacucu     1800

aagcggguga augccaaaag ccugaucaag uaucuuuccg auaaccauaa ggaccugaau     1860

gccauugaga ucguuagcgg cguggagaaa ggcaagucuu uuaacuccac acucaaaacc     1920

uacaaugauc ugaagaccau uuucagcgag gagcugcuug acucugaaau cuaucaaaaa     1980

gagcucgaag agaucauuaa ggucaucaca guguuugaug acaagaaauc cauuaagaac     2040

uaccugacca aauucuuugg gcaccuggag auccucgaug aagagaagau uaaucagcug     2100

agcaaacuua gauauuccgg auggggccgg uacucugcua agcugcuccu ggacauccgc     2160

gaugaagaca caggguucaa ccugcuucag uuccucagaa augaugagga aaaccggaac     2220

cugacgaaac ugaucagcga caauacccuc uccuuugagc cgaagauuaa ggauauccag     2280

ucuaaaagca caauugagga cgacaucuuc gaugaaauua agaaacuggc gggauccccu     2340

gccaucaaga gaggcauucu uaacagcauc aaaaucgucg acgagcuggu gcaaauuauc     2400

ggguaucccc cucacaauau uguuaucgaa auggcucgcg agaacaugac cacagaggaa     2460

ggccagaaga aggccaaaac ccggaagacg aaacucgagu cugcccugaa gaauauugaa     2520

aacucccugc uugagaaugg aaaagugccc cauagcgaug aacagcucca gucugagaag     2580

cuguaccugu acuaucucca aaacggcaag gacauguaca cccuggauaa aacagggucc     2640

cccgcuccuc uuuaucugga ccagcucgau caguacgagg ucgaccacau caucccguau     2700

agcuuucugc ccauugauuc uaucgacaau aaggugcuga cccauagaga aaacaaucaa     2760

cagaaacuua acaauauucc ugauaaggag acaguugcca acaugaaacc cuucugggaa     2820

aagcuguaca augcgaagcu caucagccag accaaauauc agcgccugac gacauccgag     2880

agaaccccug acggagugcu cacagaaagc augaaggcug gcuuuauuga gcggcaacug     2940

gucgagaccc gccagaucau caaacacgug gcccggauuc uugacaacag auucucugau     3000

accaagauca uuacacugaa aucccagcuc aucacgaacu uucgcaauac cuuccauauu     3060

gccaagauca gagaacugaa cgacuaccac cacgcucaug augccuaccu ggcugugguu     3120

gucgggcaga cacuucugaa gguguauccc aaacucgccc cggagcugau uuacggacac     3180

caugcgcacu uuaaucggca cgaagagaac aaggccaccc uccgcaaaca ucuguauagc     3240

aauaucauga gauucuuuaa caauccugac uccaaggugu cuaaagauau cugggacugc     3300

aaccgggauc uucccauuau caaggacguc auuuacaaua gccaaaucaa cuucgugaaa     3360

cgcacaauga uuaagaaggg cgcuuucuau aaucagaacc cuguugggaa auuuaauaag     3420

cagcuggccg cuaacaauag auacccccuc aaaaccaagg cccugugucu ggauacgucc     3480

aucuauggcg gauacggccc uaugaacucu gcgcuuagca ucauuaucau ugccgaacgg     3540

uucaaugaga aaaaggggaa gaucgagaca gugaaagaau uucacgacau uuucaucauc     3600

gauuacgaga aguuuaacaa caaucccuuc caguuucuga acgacaccuc cgaaaaugga     3660

uuccucaaaa agaacaauau uaacagaguc cugggcuuuu aucgcauccc gaaauacucu     3720

cucaugcaaa agauugaugg gacccggaug cuguucgaga gcaaguccaa ucuucauaaa     3780

gcuacacagu uuaagcugac caaaacacag aacgagcugu ucuuucacau gaagagacuc     3840

cugacgaaaa gcaaucuuau ggaccugaag ucuaaguccg ccaucaaaga aagccaaaac     3900

uucauucuca agcauaaaga ggaguucgac aauaucucua accagcuguc cgcuuuuagc     3960

cagaagaugc ucggaaauac cacaucucug aaaaaccuua uuaagggcua uaaugaacgc     4020

aaaaucaagg aaaucgauau ucgggacgag accaucaagu acuucuauga uaacuuuauu     4080

aaaauguucu ccuuugugaa guccggggcc ccuaaagaca ucaacgauuu cuuugacaau     4140

aagugcacag uugccagaau gcgccccaaa cccgauaaga agcugcugaa cgcuacccuc     4200

auucaccagu ccaucacggg ccuguacgag acccggaucg accuuagcaa acugggagaa     4260

gauuga                                                                4266


<210>  4488
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4488
augaagaaaa uccugggccu cgaccugggg cccaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acgagaauga aaaguccucu aucauuaaaa ucggcagcag agucauucac      120

uacggggaua accugguuaa gguggacaaa uccggaaaga ucucugccag caugaccccu      180

gaggaagauu ucuccucugg caaggggcuc agcccgaaug cgggacggac aaaacagcgc      240

uccgcuagac ggaaccugca acgcuauaag cagagacgga auaaccuuau cgagauucug      300

aaagcccaua gccucaucga caauaacuuu auuuucucug aggaaggcaa ugagacgacc      360

uuuaagacau acaaaaaccg cgcccuggcu gccaccaaug aaaucucccu ggaggauuuc      420

auuaaggugc ucuuuaugau caacaagaaa agaggcuaua agagcucucg gaaagcuaag      480

ggggacgagg auggacagcu gauugacggc auggccaucg cgauccagcu uuacgaacac      540

aaucaaacac ccgggcaguu cgucuauaac cugcucaaaa acggaaagaa aaauauuccu      600

gauuuuuacc gcuccgaccu gcagaccgag uucgauagaa ucuggaacuu ucagaaggcc      660

uuccaucaag aaagccuuuc ugacggcauu ugcgagaagc ugguggggca gaaaaagaau      720

guuacauccg cuauccucuu uaaagaguuc aagauugaaa gccuggagau caaaggaaag      780

ugggaugaaa agcgguuuuu ccgcuaugag cugagaaaca aagccgugca gcaggaacuc      840

aagcugaaug agaucgcuga augucuuguc gagauuaacg gcgagaucuc caauucuagc      900

ggcuaccugg gggccauuuc cgaccggagc aaagaacucg uguuccaaaa gaucaccguu      960

ggacaguacc uguauaaaca gauugcggcc aaccccaaua cgucucuuaa gaagcaaauc     1020

uuuuaccgcc aggacuaucu ggaugaguuc aacacccucu gggaaacaca ggcuaaauac     1080

cacaaggagc ugaccgacga acugaaaaca cagaucagag augugauuau cuuuuaucaa     1140

cggagacuca agucccagaa aggccugauu agcuucugcg aguuugaguc uaagcagauc     1200

gaagucuucg uggacgggaa gaaaagcauu aagacccgcg gaaugcgggu gaucccuaaa     1260

uccucucccc uuuuucagga guucaagauu uggcaaaucc ugaauaacau cgaaauuacg     1320

gaucucaaaa caaaggagag cagagagcug accgacgaug aacgccagcu ucuguuugag     1380

gaacucaaua uccggccuaa aauuuccaag aaggaggccc ugaaaauccu gacagaaaag     1440

ccggcucagu acgaccucaa cuauaaagau auugagggca auaagaccaa cgccaaauuc     1500

uacgaggccu acaacaagau cauccaucug agcgggcacg acgaaauuga uuuuaccgac     1560

aagacaucug acaucaauga guaucuuaaa cagauuuucu cccugcucgg aaucaagccc     1620

gaaauucugg auuuugacag cucucugaaa gaugaggacu ucgaaaagca acuucacuuc     1680

cagcucuggc aucugcugua cuccuuugag ggcgauaaca cgaaaaccgg cgacgagaag     1740

cuccugcaga aacuuaauac aaaguucggg uuuccugucg aauaugcuaa gguuaucagc     1800

aacaucgcgc ugcccgauga cuacggaucc cucucugcca aagcuauuag aaagauccug     1860

ccucaccuga aagauggcuu cagcuauggg ggacgcaagu cccggcccga cgagccgucu     1920

gccugugaau acgccggcua uagacauagc aaggcuuccc uuaccaaaga ugagaaggaa     1980

aauaaaacau acaaggacaa acucgagauu cugccuaaga acagccugcg caaucccauc     2040

guggagaaaa uucucaacca gaccaucaau guggucaacg ccgugaucgg ggauuacggc     2100

aagccugacg aaauucggcu ggagcuugcg agagaacuga agaaaucugc ugaggagcgc     2160

caagacgcca cggauuccau cagcaaggcc acagcuaaac acgaaaagua ucggaaaguu     2220

cuccagaccg aguuuggacu gaagcacauu ucuagaaaug accugauccg guacaaacuu     2280

uaucucgaac ugaagaacaa uggcuucaag acccuguacu ccgauacaua uauugccccc     2340

uacgagaucu uuagcaaaaa guucgacauu gaacauaucc uccccaaagc uaagcuguuu     2400

gaugacucuu ucagcaacaa gacccuugag cugaaauccg ugaaucucaa gaaaggggau     2460

augacagccu augacuuugu caaggaggaa uacggaacgg agggccuggc gaaauacgaa     2520

cagcgcgugc aagaucuguu ccuuaacaag aagagaaacg aggaccucga ucaggaaaau     2580

gagagccgga ucaccgacga gaaaauuucu aagacaaaaa gagauaagcu gcugaugccu     2640

gaaaacaaaa ucccgaccgg guuuauugac cgcgagcucc gggacuccca guauaucgcc     2700

aagaaggcua aaacaaugau ggaagauauu ugcaaggacg uugugaccac gaccggaagc     2760

gucacagaua gacugcgcga ggacuggcag cuuaucgaua ucaugcaaga acugaauugg     2820

cccaaauacg ccgcucucgg ccugaccgag gaguucacaa acgaagaggg gaaugugauu     2880

agaaagauca aagacuggac caagcggaac gaucaccgcc aucacgccau ggacgcgauu     2940

acgaucgccu ucacaaaaag aucccauauu caguaucuga auaaccuuuc ugcucggagc     3000

gauaaguccu cuagcaucua cggcaucgaa cagaaggagc uguaucgcga caaaaauaac     3060

aagcucagau uugccccucc cauuccuauc aaugaguucc gggcugaagc caaaaugcag     3120

cuggagaaca cccucauuuc caucaaggcc caaaauaaag ucgugacaaa gaacaagaau     3180

aaaauuucug gacagaagaa acugcaggaa acccuuacac cccgcggcca gcugcaccuc     3240

gagaccaucu acgggaagau uaaccaauau cagacggcug aaaucaaagu uggaggcacc     3300

cuggauagcg agcugaucaa caagguggcg aauaagaaaa caagagaggc ccuucugaac     3360

cggcucgcug aauuugggaa ugacccgaag aaagccuuca ccgucaagaa cacaauugac     3420

aaaaauccua ucuaccugga uaagcaccag uccuacaaga uucccgagaa agugaagauc     3480

guuuggcaag aaccugugua uaccauucgc aaaagcauca cgcccgaccu caagcuggau     3540

aaaaucauug acccuaagau caaagcccuu cuggagucua gacucaagga auguaacgga     3600

gaugcuaagc uggccuuuuc caaucuggcg gagaacccca uuuggcuuaa uaaagagaag     3660

ggcauccaga uuaaacgggu cacaaucacc gggaucagca acgcugccuc ucugcaugac     3720

aagaaagauc ucaauggcaa gccgauucug gacgaaaagg gaaacaaaau cuccguggau     3780

uucgugaaua ccggcaacaa ucaccauguu gccauuuaca aggacgagaa cgggaaucuc     3840

cacgauaacg ucgugacauu uuucgaagcu guggagagag ucaaccagaa ucugccugug     3900

guugacaaac uuuuuaagca gaaagaagga uggcaauucc uguuuaccau gaagcagaac     3960

gaguauuucg uguuccccaa ugagaucaca gccuuuaacc ccaaugaguu cgaucugcuc     4020

aacccugaca auuacuaucu gauuagcccg aaccuuuuuc gcguccagac gaugucuaag     4080

gugauguacg gcaauaacac ccugcgggac uauaaauuca gacaccaucu cgaaacaacc     4140

cugcaggaua ucaaggaacu ccgcgacauu guuuuuaaac aauucaaguc ccugagcauc     4200

uuugauaaaa ucgugaaggu ccggauuaau caccuuggga agaucgugaa aacaggagag     4260

uacuga                                                                4266


<210>  4489
<211>  4266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4489
augaaggaca uccugggccu cgaucugggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augacaacga acaauccgag aucguugaug ugggcgugag agucaauccc      120

cuuacaacgg acgaaaagac caacuucgag aaagggaagc cuauuucuac aaaugccgau      180

cggacccuga aacgcagcgc gagacggaac cuccagcgcu acaagcugag acggaaggaa      240

cuuaucgagc ugcucaaaga gcaccagcug auuuccacag acaccauccu gacagaaaau      300

ggaaagggca ccacguuuca gacccucgag cugcgcgcua aaucugccaa ggagaaaauc      360

agccuugagg agcuggccag aguguuccuc acaauuaaca agaagcgggg guauaaaucc      420

ucucgcaagg cuaaaaauga agaugaggga caacugaucg acggcaugga aauugccaag      480

agacuuuacg aggaaaaccu gaccacaggc cagcucagcc ugcagcugcu caaagagggg      540

aagaaauccc ugccggauua uuaccggagc gaccuucagu uugagcugga uaagguuugg      600

aauuuccaaa agcaguuuca uucucacauc cucaccgacg aauuuuaugc ucagcugaaa      660

ggaaagggcc agcgcgccac guccgcgcuu uuucuggcca aauaccaaau uuauacagcu      720

gagaacaagg ggagcagaga agauaaaaag cuccaggccu accaguggcg gguggacgcu      780

cugucuaagg agcugacccg cgaacaacuc gccuucguca ucacagagau uaauaacaau      840

cugaacaacu ccagcggaua ccuuggcgcg aucucugaca gauccaaaga gcuguauuuu      900

aauaacgaaa ccguggggga guaccucugg aagcagaucg ccgcuaaucc ccauaccagc      960

cugaaaaacc agguuuucua ucggcaggau uaccuugacg aauuugagca aauuuggaag     1020

acacaguccg ccuucuaucc ugaaauccug acggagucuc ucaaaaagga gcugagagau     1080

guggucauuu ucuaccagcg caagcugaaa agccagaagu cccucgugag cuucugcgag     1140

uucgaaaaua aagaaaucga gauuaagaaa gaggacggaa ccucuacaaa gaaggugauc     1200

ggccugaaag ucgcucccaa guccagcccu cuuuuucaag aguucaaaau cuggcagaac     1260

cugaauaacg uucucauucg gaagaaaggc ucuaagacca aaaaggccgc caagaaucag     1320

caggcugugc uguuugaaga agagaaaaca aucagcgaac uggaucuuga ggcgaagcaa     1380

gcccucuuca acgagcugaa ucugaaaggg aaccucaagg cugacuauug ucuggaaauu     1440

cuuggauacu cccccaaaga gugggaaaug aauuacucug ugcuggaggg caacagaacc     1500

aauaaggccc ucuaugaagc cuaccugaag aucacggauc uggaggggua ugacgcuaaa     1560

aaccaccuua aggucaaacu caacaaggau gagguggaac uggacgaucu gaaaguuccu     1620

gccagcgaga uuaaggacau ggugcaggcg aucuuuaagg aagcuggaau caauacagac     1680

auucuccauu ucaacgccga gcuggauggc aaagacuuug aaaaucaggc cuccuaccag     1740

cuuuggcauc ugcucuauag cuacgagggg gaugacucua aguccggaaa cgagcugcug     1800

uauaaacuuc ucgaaaccaa guucggcuuu aaaaaggagu acgcucaaau ccuggccaau     1860

gucagccugu cugaugacua cggcucccuc agcaccaagg cuaugcgcaa aauucacccg     1920

uauaucaagg aaaacaaauu cuccacagcc ugcgagcugg cgggguaccg gcacucuaag     1980

cacagccuua ccaaagagga aaaugauaac agagugcugc gcgacucccu cgagguucug     2040

aagaaaaauu cucugcggca gcccgugguc gaaaagauuc uuaaccagau gaucaaucuc     2100

gugaacgccc ugcugaagaa auauagaaca gagaauccua acuuuaaguu cgaugaaauu     2160

cgcaucgagc ucgcucggga gcugaaaaag aaugccgaag agagagcuaa caugacggcc     2220

aacaucaaua aagcgaagac cgaacaugag aagauuauca aaauucuuaa gaacgaauuu     2280

ggacugccca auccuacacg caacgacauc auucgguaca gacucuauga ggagcugaaa     2340

gccaauggcu acaaggaucu guauaccaac aucaaaaucc cccaagaaaa acuuuucaca     2400

aaggagauug acaucgaaca cauuaucccg aaaagccggg ucuucgauga cuccuuuagc     2460

aauaagaccc ugucuuuccg caaaacgaac cucgacaagg gggagagaac cgcuuuugau     2520

uacauggaau ccaaauucgg agccgacaag cuggaggagu acguggcucg gguugaagau     2580

cucuauaaga auaacauuau cagcaaagcc aaguaccaga aacugcuuaa gaaagagucu     2640

gaaaucggcg acggguuugu ggagagagau cugcgcgaga cacaguauau ugccaagaag     2700

gcuaaagaaa uccuccugca gauuaccccu aauguccugu ccacaagcgg cggaaucacc     2760

gaccggcuua gagaggauug ggaccugauu aacgugauga aggaacucaa ucugcccaaa     2820

uaccgcgcgc ucggccugac ggagauggaa gauagaaagu augggcaaca gguugaggug     2880

aucaucgacu ggacaaaacg gaacgaucac cgccaucacg ccauggacgc ucuuaccguc     2940

gccuucacaa agcauaauca cauucaguac cugaacuauc ucaacgccag aaagaaugag     3000

aagcauaagg aacacucuaa caucaugggc auucagcaac uggagaccau caaagugacc     3060

gauaagaaug gaaacgaaaa acggguguuu aaggcuccua ugcccaauuu ccgccagguu     3120

acaaaagccu uucuggagaa cauucuuauc agccacaagg cgaagaauaa agucgugacg     3180

aagaacaaaa auaagguggc uggguccaac aaaguccagg aagugcugac ccccagagga     3240

cagcuccaua aggagacagu uuacggcaag uaccaauauu acgugcagaa agaggaaaag     3300

auugggggca aauucgaccu ggagaccauc aagaaagucg ccaauccucu cuaucagagc     3360

cugcuuaaga agcggcugga agagaacgga ggcgacccga aaaaggccuu uacagggaaa     3420

aauucucugg cuaagaaccc caucuaccuc gaugacgccc aaaccgaaca gcugccugag     3480

aaagugaaac uuguuuggcu ggaaaauucc uucagcauuc gcaaagauau cacgcccgaa     3540

aacuuuaagg acgagaagcu cauugauaaa auccuggacg aaggagugaa gcggauucuc     3600

aaagagagac ugcaguccuu cggcaaugau gcgaagaagg cuuucucuga ccuugagaag     3660

aacccuaucu ggcugaacaa ggaaaaaggg aucacaauua agcgcgucac caucagcgga     3720

guggccaaug ccgagccccu gcacaccaaa aaggaucauc ucggcaaaga aauucuggac     3780

aaggagggga agccgauccc uguugauuuu auuuccacag gcucuaacca ccacguggcu     3840

gucuauagag acgaaaaagg aaaucuucag gagaaggugg ugagcgucuu cgaggccguu     3900

gugcgggcua accaaggccu gcccaucauc gacaaaaccu acaaucagca ucucgggugg     3960

gaauuucugu ucacaaugaa gcagaacgag auguuugugu ucccuaauga auccacggga     4020

uuuaacccca augauauuga ccuccuggau ccuaaaaaca agagaaucau uucucccaau     4080

cuuuuccgcg uccagaagcu ggccaccggc gacuauuggu uucggcacca ucuggagaca     4140

accguggaaa caaaaagcgc gaccaaggau augacguaca aaagacucuc ccugaaccac     4200

aucaagaaua uuaucaaagu ucgcauuagc caucuugggg acaucguguc ugucggagag     4260

uacuga                                                                4266


<210>  4490
<211>  4275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4490
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa cagaugagga agucuccucu aucauucggc ugggcguucg cgugaacccc      120

cucacggugg acgagaccca gaauuucgaa aaagggaaaa gcaucacaac caacgccgag      180

agaacacuga aacgguccau gcgcagaaau cuucaacggu acaagcugcg cagagaugcg      240

cucgucgagu uucugaaaga acugggauuc auuaccgacg agacaauccu cucugaacag      300

ggcaaccgga ccacguuuga gaccugccgc cugagagcua aggccgccga ggaagagauc      360

agccuugaag aacugucccg ggugcuccug augauuaaua agaaacgcgg guauaagucu      420

agcagaaaag uuaagaaaga agaggaugga gugcuuaucg acggcaugga gauugcuaag      480

aagcuguacg aagaggaucu cacaccuggc cagcuguguu cccagcugcu cgaaagcggg      540

aaaaaggucc ugccggacuu cuaucggucu gaucuucaag aggaacugga ccgcaucugg      600

uccgugcaga aacaguuuca ccccgauguu uucugcgacg ccgcuaagga ggagauuaaa      660

ggaaagaaca gaagccagac cugggccauc cucgcgaauu acuuugugug gaaagaagag      720

gucgugggcu ggaacgaucg ggaagccaag aaugagacaa uugaaaagga guguaaacug      780

guggggcuua agagaaccac gaaaggauau gagcugaaga aagaaaacua ccgcuggcgg      840

gcucaagccc ucacagagca gcugggccug gaagagaucg cugucguucu ccaggagauc      900

aaugggcaga uuaacgccuc uuccggauau cugggcgcga ucagcgacag aucuaaggug      960

cuuuacuuca aucaucaaac cgugggccag uaccagaugg ccgaacugga caagaacccu     1020

cacgcuuccc uccgcaacau ggucuuuuau cggcaagauu accuggacga guucgauaga     1080

auuugggaaa aacaggccga guuucauaag gaacuuacag cugagcugaa aaaggagauc     1140

cgcgacauga uuaucuucua ucagcggaga cucaaaagcc agaaggggcu gaucuccuuu     1200

ugcgaguucg agcgccugga aauugugguu gagaaggaug ggaaaaagca aaccaaagug     1260

aucggcugua aggucauucc ccggucucac ccucucuucc aggaauuuaa aguguggcag     1320

acccugaaug acaucaaggu ucuugggaga gagaagaaac gcaacgcccu gagcgccucc     1380

gagagccggg cucucuaccc cgaagagaag gaaauucugg cgagagagcu ggccaucaaa     1440

gaaaauauga agaaagcuga ugugcuuaag cuccuguucg agaacccuca ggagcuggac     1500

cucaauuuua aacaaaucga uggaaaccgg acaggcuucu cucuguuuuc cgccuauagc     1560

aagaugauug aaaaguacgg guaugagccg cuugacuuca aaaagcccgc cgaugaaauc     1620

auuggacagc ugcgcacgau cuuuaccgac cucggcugga auacaaaccu guucaccauu     1680

gaccugacaa aagagggcaa ggagcuugaa augcagccuu acuuuagacu cuggcaucug     1740

cuguacucuu ucgaggggga uaauaccagc acgggaaacg gcaaacucau cgaaaagauu     1800

augcagcugu gcggggucga gaaggaauau gcuguggagc uugccucccu gucuuuucaa     1860

gacgauuacg gaagccucuc cgcgaaagcu aucaagcgga uccugcccua ucugaaagag     1920

ggcaaucuuu acgacgucgc cugugaauau gccggguaca gacacagcaa gucuucccuc     1980

acaaaagagg aaauugagaa caaggugcug aagggcaaac uggaaguucu cccuaagaau     2040

agccugcgca accccguggu cgagaaaauc cuuucucaga uggugaaugu uauuaacacc     2100

aucauugaua ccuauggaaa gccggacgag auccgggugg aacuggcuag agagcucaaa     2160

aaguccgcca aggaacgcga ggaacugaca aaagcuaucg ccaagagcac cagagagcac     2220

gaggaaauuc ggaaacugcu ucaggaugag uucggcauga ugaacgucuc ccgcaaugac     2280

aucauuagau acaagcucua cgaggaacug aaagauaacg gguauaagac acuguacucu     2340

aaucaguaua ucccuaaaga gaagauuuuu agcaaggaaa ucgacaucga gcauauuauc     2400

ccccaauccc gccucuucga ugacucucug agcaacaaaa cgcuugaaua caaggcgauu     2460

aauaucgaga aaggaaacaa gaccgccuau gauuucguga aagaaaagua cggcgaggag     2520

gggcugcagc aguauauuaa ucggugcgaa ucccucuuua acgacaagaa agcuaagcug     2580

agaaaacuga agauggagca aaaagacauc ccugauggau ucaucgaccg ggaucuucgc     2640

aauacacagu acauugccaa gaaggcucug gccaugcuca acgaaaucug uagacgggug     2700

guugcgacca caggcagcau uaccgaccag cugcgcgagg auuggcagcu cgucgacgug     2760

augaaagaac ugaaucuucc caaguacgag gcccuggggu uuguggagac guaugaagau     2820

aaagacggca gaaagaucaa acggauuaag gauuggacca agagaaacga ccaccgccau     2880

cacgcuaugg augcccucac agucgcuuuc accaaagacg ccuuuaucca auacuucaau     2940

aacaagaaug ccgcucagga uucuggaaac cggaaccaca caaauauuau cggcaucaaa     3000

accagauauu uugacaaggg gcgcgcgcug cccccuauuc cgcuggagca guuccggauc     3060

gaagccaaac agcaucuuga gaagcugcuc guguccauua aggcuaaaaa caagguugug     3120

acggucaaug ugaacagaac caaaaagcgc aaaggagaaa auacaaagau ccaacagaca     3180

ccccggggcc agcugcaccu cgagaccguu uacgggagcc auaaacagua ugugaccaag     3240

auugagaagg ucaacgccuc uuuugacgcc gcuaaaaucg ccacaguguc caagagagcg     3300

uaccgcaaug cucugcuuaa acggcuggaa acguucggaa acgauccuaa gaaagccuuu     3360

accggcaaga augcccucga gaagaacccc cuguaugcug acaaauacca aacaaucaga     3420

guuccugaaa aggugcagac cgucgaguuc gagacaauuu acaccauccg gaaacccgug     3480

gauccggccc ugaaugugga caaggucguu gaugugaaag ugcgcgcgau ucuugaaaga     3540

cggcugaagg aguauggggg cgacccuaag aaggcuuuug ucaacuucug ggaaaauccc     3600

aucuggcuca acaaggagaa aggaauuagc aucaagcgcg ugucuaucag aggcauuaau     3660

aacgcccaga gcauccacgu uaaaaaggau aagaacggga auccuauuug ggacgaaaac     3720

ggaaaacaaa uccccgugga uuucgucaau acgggcaaca aucaucacgu ggccauuuac     3780

cggaagccug uucuggacaa aaagggacag gugacauuug augaggacgg aaaccucaug     3840

uaugagcugg acgaagucgu gguucccuuc uuugaggcug ugaccagagc caaucuuggc     3900

cugccgauca uugauaaaga cuaccgcaag uccgaagggu ggcaguuccu guuuagcaug     3960

aaacagaacg aguauuucgu cuuuccuaau gaaaagaccg gcuucaaccc caaggaggug     4020

gaucuccuga aucccgacaa cuacgcuaug aucucuccua aucuuuuucg ggugcaaaca     4080

auguccaaag ucauguaugg aaacaauguu gugagagauu acaaguuccg ccaccaucug     4140

gagaccacag ugaaagacau gaaggaacuc aaagauaucg ccuacaagca guauaagacg     4200

cugagcuuug gcaacuccgu cgugaaaauu cggaucaauc acauugggca gaucguuucu     4260

gugggagagu acuga                                                      4275


<210>  4491
<211>  4275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4491
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa cagaugagga agucuccucu aucauucggc ugggcguucg cgugaacccc      120

cucacggugg acgagaccca gaauuucgaa aaagggaaaa gcaucacaac caacgccgag      180

agaacacuga aacgguccau gcgcagaaau cuucaacggu acaagcugcg cagagaugcg      240

cucgucgagu uucugaaaga acugggauuc auuaccgacg agacaauccu cucugaacag      300

ggcaaccgga ccacguuuga gaccugccgc cugagagcua aggccgccga ggaagagauc      360

agccuugaag aacugucccg ggugcuccug augauuaaua agaaacgcgg guauaagucu      420

agcagaaaag uuaagaaaga agaggaugga gugcuuaucg acggcaugga gauugcuaag      480

aagcuguacg aagaggaucu cacaccuggc cagcuguguu cccagcugcu cgaaagcggg      540

aaaaaggucc ugccggacuu cuaucacucu gaucuucaag aggaacugga ccggaucugg      600

uccgugcaga aacaguuuca ucccgauguu uucugcgacg ccgcuaagga ggagauuaaa      660

ggaaagaacc gcagccagac cugggccauc cucgcgaauu acuuugugug gaaagaagag      720

gucgugggcu ggaacgauag agaagccaag aaugagacaa uugaaaagga guguaaacug      780

guggggcuua agcggaccac gaaaggauau gagcugaaga aagaaaacua cagauggcgc      840

gcucaagccc ucacagagca gcugggccug gaagagaucg cugucguucu ccaggagauc      900

aaugggcaga uuaacgccuc uuccggauau cugggcgcga ucagcgaccg gucuaaggug      960

cuuuacuuca aucaccaaac cgugggccag uaccagaugg ccgaacugga caagaacccu     1020

caugcuuccc ucagaaacau ggucuuuuau cgccaagauu accuggacga guucgaucgg     1080

auuugggaaa aacaggccga guuucacaag gaacuuacag cugagcugaa aaaggagauc     1140

agagacauga uuaucuucua ucagcgccgg cucaaaagcc agaaggggcu gaucuccuuu     1200

ugcgaguucg agagacugga aauugugguu gagaaggaug ggaaaaagca aaccaaagug     1260

aucggcugua aggucauucc ccgcucucau ccucucuucc aggaauuuaa aguguggcag     1320

acccugaaug acaucaaggu ucuugggcgg gagaagaaaa gaaacgcccu gagcgccucc     1380

gagagccgcg cucucuaccc cgaagagaag gaaauucugg cgcgggagcu ggccaucaaa     1440

gaaaauauga agaaagcuga ugugcuuaag cuccuguucg agaacccuca ggagcuggac     1500

cucaauuuua aacaaaucga uggaaacaga acaggcuucu cucuguuuuc cgccuauagc     1560

aagaugauug aaaaguacgg guaugagccg cuugacuuca aaaagcccgc cgaugaaauc     1620

auuggacagc ugcggacgau cuuuaccgac cucggcugga auacaaaccu guucaccauu     1680

gaccugacaa aagagggcaa ggagcuugaa augcagccuu acuuucgccu cuggcaucug     1740

cuguacucuu ucgaggggga uaauaccagc acgggaaacg gcaaacucau cgaaaagauu     1800

augcagcugu gcggggucga gaaggaauau gcuguggagc uugccucccu gucuuuucaa     1860

gacgauuacg gaagccucuc cgcgaaagcu aucaagagaa uccugcccua ucugaaagag     1920

ggcaaucuuu acgacgucgc cugugaauau gccggguacc ggcacagcaa gucuucccuc     1980

acaaaagagg aaauugagaa caaggugcug aagggcaaac uggaaguucu cccuaagaau     2040

agccugagaa accccguggu cgagaaaauc cuuucucaga uggugaaugu uauuaacacc     2100

aucauugaua ccuauggaaa gccggacgag auccgcgugg aacuggcucg ggagcucaaa     2160

aaguccgcca aggaaagaga ggaacugaca aaagcuaucg ccaagagcac ccgcgagcac     2220

gaggaaauua gaaaacugcu ucaggaugag uucggcauga ugaacgucuc ccggaaugac     2280

aucauucgcu acaagcucua cgaggaacug aaagauaacg gguauaagac acuguacucu     2340

aaucaguaua ucccuaaaga gaagauuuuu agcaaggaaa ucgacaucga gcacauuauc     2400

ccccaaucca gacucuucga ugacucucug agcaacaaaa cgcuugaaua caaggcgauu     2460

aauaucgaga aaggaaacaa gaccgccuau gauuucguga aagaaaagua cggcgaggag     2520

gggcugcagc aguauauuaa ucggugcgaa ucccucuuua acgacaagaa agcuaagcug     2580

cgcaaacuga agauggagca aaaagacauc ccugauggau ucaucgacag agaucuucgg     2640

aauacacagu acauugccaa gaaggcucug gccaugcuca acgaaaucug ucgcagagug     2700

guugcgacca caggcagcau uaccgaccag cugcgggagg auuggcagcu cgucgacgug     2760

augaaagaac ugaaucuucc caaguacgag gcccuggggu uuguggagac guaugaagau     2820

aaagacggcc gcaagaucaa aagaauuaag gauuggacca agcggaacga ccauagacac     2880

cacgcuaugg augcccucac agucgcuuuc accaaagacg ccuuuaucca auacuucaau     2940

aacaagaaug ccgcucagga uucuggaaac cgcaaccaua caaauauuau cggcaucaaa     3000

acccgguauu uugacaaggg gagagcgcug cccccuauuc cgcuggagca guuccgcauc     3060

gaagccaaac agcaccuuga gaagcugcuc guguccauua aggcuaaaaa caagguugug     3120

acggucaaug ugaaccggac caaaaagaga aaaggagaaa auacaaagau ccaacagaca     3180

ccccgcggcc agcugcaucu cgagaccguu uacgggagcc acaaacagua ugugaccaag     3240

auugagaagg ucaacgccuc uuuugacgcc gcuaaaaucg ccacaguguc caagcgggcg     3300

uacagaaaug cucugcuuaa acgccuggaa acguucggaa acgauccuaa gaaagccuuu     3360

accggcaaga augcccucga gaagaacccc cuguaugcug acaaauacca aacaauccgg     3420

guuccugaaa aggugcagac cgucgaguuc gagacaauuu acaccaucag aaaacccgug     3480

gauccggccc ugaaugugga caaggucguu gaugugaaag ugcgggcgau ucuugaacgc     3540

agacugaagg aguauggggg cgacccuaag aaggcuuuug ucaacuucug ggaaaauccc     3600

aucuggcuca acaaggagaa aggaauuagc aucaagcggg ugucuauccg cggcauuaau     3660

aacgcccaga gcauccaugu uaaaaaggau aagaacggga auccuauuug ggacgaaaac     3720

ggaaaacaaa uccccgugga uuucgucaau acgggcaaca aucaccacgu ggccauuuac     3780

agaaagccug uucuggacaa aaagggacag gugacauuug augaggacgg aaaccucaug     3840

uaugagcugg acgaagucgu gguucccuuc uuugaggcug ugacccgggc caaucuuggc     3900

cugccgauca uugauaaaga cuacagaaag uccgaagggu ggcaguuccu guuuagcaug     3960

aaacagaacg aguauuucgu cuuuccuaau gaaaagaccg gcuucaaccc caaggaggug     4020

gaucuccuga aucccgacaa cuacgcuaug aucucuccua aucuuuuucg cgugcaaaca     4080

auguccaaag ucauguaugg aaacaauguu gugcgggauu acaaguucag acaucaccug     4140

gagaccacag ugaaagacau gaaggaacuc aaagauaucg ccuacaagca guauaagacg     4200

cugagcuuug gcaacuccgu cgugaaaauu cgcaucaauc auauugggca gaucguuucu     4260

gugggagagu acuga                                                      4275


<210>  4492
<211>  4278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4492
augagacgga acauccuggg ccucgaccug gggaccacaa gcauuggauu cgccaaggug       60

aucgaggaug aagacuacca aaauuccgcu gucgagcuua uuggcguucg cgugaacccc      120

cugacgaccg augaacagac aaauuuugag aaagggagac cugugucugu caacgccgac      180

cggacccuca agcgcggaau gagacggaau cuggaucgcu aucagcuuag acggcaccau      240

cugaucgaag ugcucaaagc gaaccacauu aucacaguug acaccaagcu ggcugaggau      300

ggcaaaaaua caacccaugc cacguacgcc gugcgcgcuc uggccgcuac cgccgagauc      360

ccgcucgaag cguucgccag aguccugcuu gcuauuaaca agaagcgggg guauaaaagc      420

ucccgcaagg ccaaaaauga ggacgaagga cagcugaucg auggcauggc uguggccaag      480

agacucuacg aggagaaccu gacacccggc cagcuuucuu aucugcuccu gaaagaaggg      540

aagaaguacc ugccugacuu uuaucggagc gaucucaaug cggagcugga caaaguuugg      600

uccuuccaaa agcaguuuua ccccgaaauu cuuaccgagg aguucaaaaa gcaccuggaa      660

ggaaaaggcc agcgcgccac aucugcugcc uuuugggggc aguacggauu caacaccgcu      720

gagaauaagg gcagcagaga agagaaaaag cucaaggccu aucaauggcg guccgaagcc      780

guggaucagc agcugacgaa agaggaaguc gcuuacguga ucacagagau uaacaaugac      840

cuuaacaaga gcucugggua ucugggagcg aucuccgaca gaagcaaaga gcucuuuuuc      900

aacaagcaga ccgugggcca auaccuguau cagcagcuga aacaaaaucc ucauacacgc      960

cucaagggcc aggucuuuua ccggcaggau uaucuggacg aguucgaaac cauuugggaa     1020

acccaggcca aguuucaccc cgagcuuaca gcugcccuga aagaagagau cagagauguu     1080

aucauuuucu accaacgcaa gcucaaaucu cagaaggggc ugaucuccuu cugcgaguuu     1140

gaaagcaaag agauugaagu ggagaagaac ggacuuaaga ucaaaaagcg gauuggccug     1200

agaguggccc cuaaaucuuc cccgcuguuc caggaguuua agaucuggca gguccucaau     1260

aaccuggagu uccgcaauaa aagcuccaag gaaguguuug uucccgacau gacggcuaag     1320

caacaacucu ucgaugaacu gaacaucaaa gggaaucuuc uggccucuga ggcgauuaag     1380

auccucggau acaaaaccaa ggacugggaa gcuaacuauc acacacugga gggcaaucgg     1440

accaacaaaa gccuguacga ugccuaucuu aagauucucg agcuggaagg guacgacgag     1500

gaucugcuca aacuguccgg aagcgacgau aucgacgugu cuaagauuga aaaguccgcc     1560

agcgagauca aacagauggu ccaugcugug uuugaaguuc uuggcaucaa uucuacaauu     1620

cuggacuucg augccgaggc ugacggcaag gccuuugagc agcagaccgc guauagacuc     1680

uggcaucugc uguacagcgc cgaagaugac aacaaaaagu aucuucagga ggauaucuuc     1740

auuuacgggc acgacaauau cggacucaaa aagaagcugu gugaacgcuu uggcuuccgg     1800

ccugagcaca cgaaaauucu ggugaacguc gcuuuuuccg gggauuacgg aucucucagc     1860

uccaaggcca ugagaaaaau cuaucccuuc auuaaggaac ugacauacga caaggcuugc     1920

cgcgaggccg gcuaucggca cagcgcgucu ucccuuagca aagaggaaau cgccaccaga     1980

ccucugcaga agcggcucgg gcugcugaaa aagaaugagc uucgcucucc cguggucgag     2040

aaaauccuca accagauggu gaacguugug aaugcucuga uugaugacga aaacaagaga     2100

cggaaagcca gaggccuguc cgaugacuuc cauuuugaug agauccgcau ugaacucgcu     2160

cgggagcuga agaagaaugc caaagaaaga gccgagauga ccacaaacau caaugcugcg     2220

aagcaacuuc acgaaaagau uaucaaaauc cugcagaccc aguucggagu cagcaacccg     2280

ucccgcaaug acauuaucag auacaaacuc uaugaggagc ugaagaacaa uggcuacaaa     2340

gaccuguaua caaacacgua cauuucuccu gauauccuuu uuagcaagca aauugacauc     2400

gaucauauca uuccccagau gucccucuuc gacgauucuu uuagcaauaa gaccgugguu     2460

uuccggaaag acaaccagga uaaggggaau aaaacagccu acgacuauau ccugggaaag     2520

uacggcgaac uggcucucga ggaauauauu ucccgcauca ccagccuguu ugagcuuggg     2580

aaaaagaaca aggaagaggg aauuaauaaa gccaaguucc agaaacugca aaagagacuc     2640

aucgauaucg gcgacggguu uauugagaaa gaucugcggg aaucucagua caucgccaag     2700

aaggcuaaaa acaugcugua ugccauuugu cgcuccgugg ucgcgacaag cggcgagauc     2760

accgcuagac uucgggaaga cugggaucug gugaacguga ugcaggagau uaaucucccu     2820

aaguuccgcg cccugggacu cacggaaaug guugagaaaa aggacggcgg gcagaaagag     2880

agaaucauug acuggaccaa gcggaacgau caccgccauc acgccaugga cgcucugaca     2940

aucgccuuua ccagacacuc ucauauccaa uaccuuaauc accugaacgc gcggaaggau     3000

gaggcuucca aauggcaucc cgucgugagc aagauugaag aaaaagagac agaccucgug     3060

uaugaugacc ugggaaauag aaagcgccgg uucaaagccc ccaugccuaa cuuuagagag     3120

gucgugaagg aacaccugga gaauguucuu aucucucaca aggccaaaaa caaggugguc     3180

accaaaaaua agaacaaaau uaagacggcu aaaggcgaaa agaucaagac agagcugacc     3240

ccgcgcgggc agcuccauaa agaaacaauu uacggacggu acagauauua cgagagcaag     3300

gaggaaaaag ugggccccaa guucgaugcc gagaccaucg ggaugguugu gaauccucag     3360

uaucgcaaac ugcuccugga acggcuuacc acaaacggca augaccccaa gaaggcuuuc     3420

ggaggcaaaa acacgcuguc caagaauccu cucuacaucg augaggccaa acaggaacug     3480

cugcccgaga agauucuucu gagauggcuc aacgacgauu auagcauccg caaagacguc     3540

accccggaga accugaagga cgugaagaca auugaaaaag uucucgauga ggggaucaag     3600

aaaauucugc uuaagcggcu gguggacuuu ggaaaugauc cuaaaaaggc guucucugac     3660

cuggauaaga accccaucug gcucaaugaa gccaaaggca ucaccauuaa gagagucaca     3720

aucuccgggg ugaaaaacgc ugaggcccug cacuacaaga aagaccauuu uggaaaugcu     3780

auucuuaacg aaaauggcaa gcugaucccu agcuccuucg ugucuaccgg gaacaaucac     3840

caugucgcca uuuaucggga ugccgagggc aaccuccaag agcgcguugu gagccuguuu     3900

gacgcugugc agcucgucaa ugcgggagaa gccgugaucg auagagagua caacaaaggc     3960

auuggguggc aguuccuguu uacgaugaag caaaaugaaa uguucguuuu ucccaacgag     4020

aagacaggau ucgacccuaa agagaucgau cuucugaaua ccgacaaccg gaagcugauc     4080

ucccccaauc ucuuucgcgu gcagaaaauu gcuucuaaga acuacuucuu cagacaccac     4140

cuggaaacca acgucgagga acggaaagag cuuaaggaua ucguguauaa gccgcagcug     4200

ggccucaaug ccauuaaaga cguugugaag gucagaauca accaucuggg gaaaauuauc     4260

agcgugggag aauacuga                                                   4278


<210>  4493
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4493
augcccagaa agaacauccu gggccucgac cuggggacca caagcauugg auucgccaaa       60

aucauugagg augacgauua ccagugcucc ucuguggaac uuaucggcgu ccggguuaau      120

ccucugacga ccgacgagca aacaaacuuu gaaaagggga aaccggugag cgugaaugcu      180

gaucgcaccc ucaagagagg aaugcggcgc aaccuggaca gauaucagcu ucggcgcgau      240

uaccugauug agauccucag aacacaccau aucauuaccg ccgacacaaa acuggcggaa      300

gauggcaaga auaccacgca caccacauau gcucugcggg cccucgccgc uaccgccgag      360

aucccccugg aggcuuucgu ccgcgugcuu cuggccauua acaagaaaag aggguacaag      420

uccucucgga aagcgaagaa ugaagacgag ggacagcuca ucgauggcau ggccauugcu      480

cgcagacugu augaagagag ccuuacaccu ggccaguuuu ccuaccaacu gcucaaagag      540

gggaagaagu aucugcccga cuucuaccgg ucugaucuga acaccgaacu caauaaaguu      600

uggaacuuuc agaagcaguu cuacccugag auccugacgg acgaauuuaa acgccaucuu      660

gagggaaagg gccagagagc cacagcugug gccuucuggg ggcaacacgg auuuagcaug      720

gcggaaaaua aaggcucccg ggaggagaag aaacugcaug ccuauaagug gagaagcgaa      780

gcugcccaca agcagcucac caaagaggaa gucgcuuucg ugauuacaga gaucaacaau      840

gaccugaaca agucuuccgg guaccuugga gccaucagcg aucgcucuaa agaacuguuu      900

uucaauaagc agaccguugg ccaguaucuc caccaacagc ugaaagagaa cccccauacc      960

cggcugaagg gccagguguu uuacagacaa gacuaucucg augaguucga aacaauuugg     1020

gagaugcagg ccaaguucua cuccgcucug acgcuugaac ugaaagagga aguccgcgac     1080

guggugaucu uuuaucagcg gaagcucaaa agccagaugg ggcugauuuc ucuuugugag     1140

uucgagucca aggaaaucga gauugagaaa aacggagaaa aggcgaagaa aagaaucggc     1200

cugcgcgucg ccccuaagag cuccccgcuc uuucaagagu ucaaaaucug gcagguucug     1260

aauaaccugg aauuucggaa uaagaaaacc aaggaggugu ucgugcccga aauggcugcc     1320

aagcagcagc ucuuugauga gcugaacauu aaagggaauc uucuggccuc ugaagcuauc     1380

aagauucucg gauacaaaac aaaggacugg gaggccaacu accacacccu ggagggcaau     1440

agaacaaaca aaagccugua ugaugcgugg cuuaagaucc ucgaacugga gggguacgac     1500

gaaaaucugc ucaaacuguc cggaagcgau gacauugaug ucucuaagau cgagaagccu     1560

gcucuugaaa uccgccaaau ggugcuggcc guuuucgagg ugcucggcau uuccggcgcc     1620

auccuggacu uugacgcuga ggccgauggg aaggcuuucg aacagcagcc cgccuaucag     1680

cuguggcauc uucucuacag cgcggaggac gauggaaaga aauauucuca agaagacauu     1740

uuuaccuacg gccacgauaa caucgggcug aagaaaaagc ugugcgagaa guucggauuu     1800

cggccugaac acacgaaaau ucucgucaau gugguuuuca gcgacgauua uggcaaccug     1860

uccucuaagg ccaugagacg caucuacccc uucauuaaag agcuuacaua cgacaaggcu     1920

ugugccgagg cuggguaucg gcauagcgcc gcgucccuga ccaaagaaga gaucgccgcu     1980

agaccgcucc agaaucggcu ggaucugcuu aagaagaacg aacuccgcag cccugugguc     2040

gagaaaaucc ugaaucagau ggugaacguc gugaacaccc ugguugacga ugagaaucag     2100

aacagaaagg cccggggccu cucugaagac uuucacuucg augagauuag aaucgaacug     2160

gcucgcgagc uuaaaaagaa ugccaaagaa cgggccgaga ugacaaccaa cauuaauaca     2220

gcuaagcaau cccaugagaa gaucauuaga auccugcaga cggaauuugg agugcccaac     2280

ccuagccgca augacaucgu cagauacaaa cucuaugagg aacugaagaa caauggcuac     2340

aaagaccugu auaccaacac auacauuucu cgggauaucc uuuucuccaa gcagauugac     2400

guggaucaca ucauucccca aaugagccuc uuugacgauu ccuucucuaa uaaaaccguu     2460

guguuucgca aggacaacca ggauaaaggg aauaagacag cguaugacua cauccuggga     2520

aaguacggcg agcaggcccu ggaagaguau aucagcagaa uuaccucccu cuucgagcug     2580

gggaaaaaga acaaagaaga gggaaucggc aaggcuaaau uucagaaacu ucaaaagcgg     2640

cuggucgaua uuggggacgg cuucaucgaa cgcgaucuca gagagucuca guacauugcc     2700

aaaaaggcca auaacaugcu guaugcuauc ugccggagcg ugguguccac gagcggagaa     2760

aucaccgccc gccugagaga ggacugggau cuuguuaacg ucaugcagga gauuaaucug     2820

cccaaauuuc gggcgcucgg ccugacagaa augguggaga agaaagacgg ggcucagaag     2880

gaacgcauca ccgacuggac aaagagaaac gaucaucggc accacgccau ggacgcccuc     2940

gcuauugccu ucaccagaca uucucacauc caauaccuga aucaucuuaa cgcgcgcaaa     3000

gaugaggcua auaaguggca cccugugguc uccaaaauug aggaaaagga gacggugcug     3060

aucuaugacg aucucggaaa ccggaaaaga aaguuuaagg ccccgaugcc caauuuccgc     3120

gaaguuguga aagagcaccu ggaaaacguc cugauuagcc auaaggccaa aaauaaggug     3180

guuaccaaaa acaagaauaa gaucaaaaca gugcggggcg agaaggucaa aacagagcuu     3240

accccuagag ggcagcugca caaggaaacc aucuacggac gcuaucggua cuacgagucu     3300

aaagaagaga agauuggccc caaauucgac gcugaaauga ucgggauggu gacaaacccu     3360

caguauaaga agcuccugcu cgagagacug gccgcuaaug gcaacgaucc caaaaaggcc     3420

uuuuggggca aaaaugcgcu uaacaagaac ccgcuguacg uugacgaggc ccgccaggaa     3480

cuccugccug agaaaauucu gcuucggugg cugaaugaug acuauuccau cagaaaggau     3540

gugacgcccg aaaaccucaa ggacauuaaa accaucgaga agauccugga caaaggaguc     3600

aagaaaauuc uccugaagcg gcuugcugau uucgggaaug acccuaagaa agccuuuagc     3660

gaucuggaca agaaccccau cuggcaaaau gaagcuaaac gcauugccau caagagagug     3720

acaauuucug gaaucaaaaa cgccgcuccu cugcaucaca agaaggauca ucucggcaau     3780

gacauccugg augagaaagg gaagcagauu cccagcuccu ucgugagcac cggaaacaau     3840

caccacgucg cgaucuaccg ggacgccgag ggcaaccuuc aggaacgcgu ugugucucug     3900

uuugaugcug ugcaacucgu caaugggggc gagccgguga uugacaaaga auauaacaag     3960

ggaaucggcu ggcaguuccu guuuacaaug aaacagaaug agauguucgu uuuuccugau     4020

gagaagaccg gguucaaccc cagagaagug gaccuccugu ccagcggaaa ucggaaacuu     4080

auuuccccca accuguuuag aguccagaag cuggccucua agaauuacuu cuuccgccau     4140

caccucgaga cgagcgugga agagaaaaag gaacugaaaa acaucgccua uaagccucaa     4200

cuuggccuga acgcucucuc caauauuguu aaagugcgga ucaaccauau cggggagauu     4260

gucucugugg gagaguacug a                                               4281


<210>  4494
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4494
augaagaaca uccugggccu cgaccugggg auuaccagca ucuccuucgc ccacauugug       60

gaggaugaaa auaaagagaa gucugaaauc aaagagcuug gagucagaau uguuagccug      120

acaacggacg aacaguccga uuuugagaag ggcaaaucua ucaccacaaa cgcuaaucgg      180

acccucaagc auggggcccg ccugaaccuu gacagauacc aacagcggcg caaguaucug      240

aucgaucucc ugcagaaagc gaaucugauu acaagcuccu cuauccucgc ugagaacgga      300

aagaauacca cacacagcac cuggcagcug agagccaaag ccgugacgga acggauugag      360

aaggaagagu ucgcucgcgu gcuucuggcc aucaacaaaa agagaggcua caaguccagc      420

cggaaagcua agaccgagga cgaagggcaa gccauugaug gaauggcgau cgccaaacgc      480

cucuaugacg agaaucugac acccggccag cuuucucugc agcuccugca gcaaaacaag      540

aaacugcucc cugauuuuua cagauccgac cugcagcggg aguucgaucu ugucuggaau      600

uuucagaagc aguucuaucc ggacauucug accgauagcu uuuacaaaga acuccaaggc      660

aaggggaagg acgcuacauc uaaagccuuc uccaagcgcu aucauuuuga caccgcugaa      720

aacaaaggaa gcaaggaguc ugugagacug caggccuacc aguggcgggc ggaggccauc      780

uccaaacaac uuagcaagga agagguugcu uacgugcuga cggaaaucaa uaacaaucuc      840

aacaacgccu ccggcuaucu gggggcuauu ucugaucgca gcaaggagcu guacuucaau      900

agacagacag ucggacagua ucucuacgcc cagcugcaag aaaaccggca caauucccuu      960

aaaaacaagg uguuuuauag acaggacuac cuggaugagu ucgagcgcau cugggaaacc     1020

caggccagcu uucauaaaga gcucacagac gaacugaaga aacagauucg ggauguugug     1080

aucuucuauc aaagaaaacu uaagucucag aaaggccuga uuuccuucug cgaguuugaa     1140

agcaaggaga ucgagaucga aaaagacggg aagaccauua ccaaaacaau cggagcucgc     1200

gucgugccca agucuagccc ucucuuccag gaguuuaaga uuuggcagau ccugaauaac     1260

gugacgugua aacggaaggg cauuagaaag aagaaauccu cugcuaagag caccgaacug     1320

gaucucuuca augagcccuc ccaaacaauc uuuagccugg acauggagug caaacagcuu     1380

cuguucgaug aacucaaccu gaagggcgac cugaagucug auaaaguccu uaagcuccug     1440

ggguacuccc cucaggagug ggaaaucaau uacaaccagc uggagggaaa ucgcacccaa     1500

aaagcccucu augaagcgua ccugaagauu guugagaugg aggcccacga cgugaaagac     1560

auccuucaga uuaagagcgc caaggaugac uggucucugg augaaagccc ccucuccgcu     1620

uccgagauca aagaaaaggu gaaagacauu uuucaggccc ugggcaucug uacaaagauu     1680

cuguauuucg auccucuucu cccggucaaa gaguuugaaa agcaagacuc uuaccagcug     1740

uggcaucugc ucuauagcua cgaguccgau gacucuacca gcgggaacga gacgcuguau     1800

cggauccuug aaaagaaaua cgcguucaag agagagcacg cucgcauccu ggccaaugug     1860

gcccuccagg augacuacgg aucccugagc acaaaagcua uucggaagau cuaucccaac     1920

auuaaagaaa aucaguacuc uaaggccugc gagcuggcug gcuauaagca cuccaaacuu     1980

agccucacca ccgaggaacu ggaggccaga gaacugaaga acaucauucc ucuccugaaa     2040

aagaacgcgc uucgcaaucc cguuguggag aaaauccuga accaaaugau caaugucgug     2100

aacgcccuca uugaaaagaa cucugagcgg gaugcugagg ggaaaaucac aaaguacuuu     2160

cauuucgacg aaauuagaau cgagcuggcc cgggaacuga agaaaaaugc ucagaagcgc     2220

uaugagauga cccagaauau uaacaaagcc aaacuugaac accagaaaau cuccgagauc     2280

cuccaaaaga aguuuggaau uaaaaauccu acaaagagcg auaucauuag auaccggcug     2340

uaucaggagc uggaacacaa cggcuacaaa gagcucuaca cgaaugcgcc caucgccaga     2400

gacaugcugu ucucuaagaa cauugaaauc gagcauaucg uuccgaaagc ucgcguguuc     2460

gacgauagcu uuuccaauaa gacccuuaca uuccaccgga uuaacagcaa uaagggcgaa     2520

uauaccgccu uugacuacau cacaucucug aacuccgagg aggaacucaa ccaguaucug     2580

accagagucg agaaugcuua caaaaccaag agcauuuccc ugacgaaaua uaagaaccuu     2640

cugaaaaagg ccucugaaau cggggaugga uucauuaauc gcgaccucag agauacacag     2700

uacaucgcca agaaagcuaa ggagauucug uuucaaguga ccaaaaacgu ccucagcaca     2760

uccggcucua ucaccgaccg gcugcgcgaa gauugggggc uuguggacgu uaugaaggag     2820

cugaauaugc cuaaauauca gagccucgga cugacggagg uggaagagag aaaggaaggc     2880

aacaagguca cagugaucaa aaauuggacc aagcggaacg aucaucgcca ccaugcgaug     2940

gacgcccuga caguugcuuu caccaaaccc uccuacauuc aguaccuuaa ucaccugaac     3000

gccagaaagg augagaauaa caaaaauuau ucugugaucc ucgccauuga ggaaaaggag     3060

accaucaaag ucccuacaaa caaugggaag aacaagcggg uguuuauuga acccaucccc     3120

aacuuccgcc aaguggcuaa aaagcaccug gaggaaaucu uuauuagcca uaaagccaag     3180

aauaaaguug ucacgaagaa cgugaauaag ccugcgggca ccgacaaaca gcagaucaca     3240

cucaccccga gaggacagcu gcacaaggag acaauuuacg gcaaauauca auacuaugug     3300

aacaaggagg aaaaagucgg ggugaaguuc gaugagcgga ccaucacgaa gguuuccaau     3360

ccccuuuacc gcgaagcucu gcucaaaaga cugcaggcca acgacaauga cccuaagaaa     3420

gccuuuacag gaaagaacgc ucugagcaaa aaucccauuu aucuugauga gaccaagacc     3480

aagacacugc cugaaaaagu gaaccucacc uaccuggagg aggacuucuc uauccggaag     3540

gauaucuccc ccgacaauuu uaaagaucuc aagagcauug aaaaagucau cgaccagggc     3600

gugaagcgca uucugaucaa aagacuucag gauuacgaca acgauccgaa gaaggccuuc     3660

guugaccugg agaaaaaucc uauuuggcug aacaaggaaa aagggaucgc gauuaagcgg     3720

gugacaaucu cuggagucuc caaugcucaa ccccuccaua ucggcaaaga ucaccugggg     3780

aagacgaccc uuaacaagga gggcaaugaa auuccugugg acuauguuag cacaggaaac     3840

aaccaccaug uggccaucua cagagacaaa gagggcaauc ugcaggagca gauugucucu     3900

uucuuugaag ccguggugcg cacccagcaa gggcucccca ucauugauaa gacauauaaa     3960

caggaggaag gauggcaguu ccuguuuacc augaagcaga acgagauguu cgucuuuccu     4020

aaugcuacga ccggcuucaa ccccgccgag aucgaccucc uggauccgaa aaauaagaaa     4080

cuuaucuccc cuaaccuguu ucggguucaa aagauugcua caaaggacua cuucuuuaga     4140

caccaucugg aaaccaaugu ggagacagau aacgugcuca agaauaccac auggaagcgc     4200

gaagggcuga gcggacuuaa agacaucguc aaggugcgga uuaaccaccu gggcgauauc     4260

guuuccauug gggaguauug a                                               4281


<210>  4495
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4495
augaagcaca uccugggccu cgaccugggg accaacagca uuggaugggc ccuuaucaaa       60

caggauuucg agaauaagca aggcgaaauu cuggggaugg gauccagaau cauucccaug      120

ucucaggaca uccucggcga uuuugggaaa ggaaacagcg ugucccagac agcugagcgg      180

acgaaguacc gcucugucag acggcugcgc gaaagauucc uucugcggcg cgagagacuc      240

caucggaucc ugaauguucu gggcuuucuc ccugaacacu augccagcca gauugacuuc      300

accaaacgcu uuggcaaguu caaggaggag acagaaccga aacuggugua cuccaacgag      360

ggguuuaucu ucaagaaauc uuuugaagag augcuuagcg auuucaagac ccaucaaccc      420

cagcugcucg aggacaauaa aaagauuccu uaugauugga caaucuacua ucugagaaag      480

aaagcgcuuu cccggaagau ugaaaaagag gaacuggcuu ggauccuccu gaacuuuaau      540

cagaagcgcg gauacuauca gcugagaggc gaggaagagg aggaaaaccc caauaaacuc      600

auugaguucu acagccugaa gaucguggac guccuugccg augaaccuca aaaagggaag      660

ucugacaucu gguacucccu gauucucgag aacggaugga ucuaucggcg cagcucuaag      720

acaccccugu uugauuggaa agaaaagacc agagacuuca uugugaccau cgaucuuaau      780

gacgacggcu ccguuaaaac ggauaaggag gggaacgaga aacggagcuu cagagccccu      840

ggagaaaaug acuggacccu ggugaagaag aaaacagagc aggaaauuga uucuucccgc      900

aagaccgucg gcacauacau cuaugagacc cuccugaaaa acccgaagca gaaaaucaag      960

ggcaagcugg ugcggacgau ugaaagaaaa uuuuacaaga cagagcucaa acagauccug     1020

gagaagcaaa aagaguucca ccccgaacuu cgccaggacg aucuguauag cgacugcguu     1080

cgggaacucu acaagaauaa cgaggcucau cagcugaccc uuuccaagaa agauuuugug     1140

caccuguuca uggaggacau uaucuuuuau caaagaccuc ucgggucuca aaagagcucc     1200

auuagcaacu guacacugga guuccgcaaa uacaaggaug aaaauggaac cgaacacacc     1260

caguaccuga aagccauccc caagucuaac ccuuauuacc aggaguuucg gaucuggcaa     1320

uggauguaua aucucaacau uuacaaaaag gaagacgaua caaaugucac gaaggacuuc     1380

cugaacacca aagcugacuu ugagucccuu uucgaguuuc ugaauaacag aaaggaagug     1440

gaucagaaag cccuccugaa gcauuucaaa cugaaugaca agacacaccg cuggaacuau     1500

guggaggaua agaaauaccc cugcaaugaa accaagacaa ugauccuuag ccggcucgac     1560

aaagucgaga acauuucuga ugacuuucug accagagaaa uggagcagaa gaucuggcau     1620

auuaucuaua gcguuaauga uaaaguggag uacgaaaagg cgcugaaguc cuucgccggc     1680

aaaaacaauc ucgaggacaa cucuuucuuu gaggcuuuca agaaauuucc gccuuucaag     1740

agcgaauacg gggccuuuuc cgaaaaagcu auuaagaagc ugcuuccccu gaugcgcaug     1800

ggaaaauucu ggaacuauaa uaacaucagc gagcagucua agucccggau ccagaaaauu     1860

aucacgggcg aguacgauga aaauauuaag gacaaaguga gagagaaggc cauccaccuc     1920

acaaccgaaa ccgauuuuca ggggcugcag cuguggcuug uccaguauau uguguacgga     1980

cggcacagcg aggcggccau gcucggcaaa uggaacucug cugacgaucu ggaggaguuc     2040

cugaaggaau uuaagcaaca uucccuccgc aauccuaucg uugaacaggu gaucacagag     2100

acccugagag ucgugaaaga cauuuggcag aaguauggca acggggccaa agacuucuuu     2160

cgggaaaucc acauugagcu uggaagagag augaagcugc ccgcugagga acgcgaacgg     2220

cucacaagac agaucagcga gaaugaaaac acgaauuucc gcauuaaagc ccugcuggcc     2280

gagaugaugg augacaacuc cguugagaau gugagacccu uuucuccuau gcaacaggaa     2340

auccuuaaga ucuacgagga aggcguccuc aacagcgaua uugagaucga agacgauauu     2400

cugaagaucu ccaaaaccgc ucagccgucu agcuccgacc ugaagcggua uaaacucugg     2460

cuggagcaaa aguacaaaag ccccuacaca gggcagauua ucccucuuaa uaagcuguuc     2520

acccccgagu augaaaucga gcauauuauc ccucagucuc gcuacuucga ugacuccuuu     2580

acaaacaaga uuaucuguga aagcgcggug aauaaacuca aggauaacua uauuggacug     2640

gaguucauca aaaagcuggg cgggucuauu gucgagugcg gcaacggaaa auccguggcc     2700

auccuuaagc acgacgcuua cgaagauuuu aucaagaaac auuaugccaa uaacagaagc     2760

aagcggaaua aacuccugcu ggaggacauu cccgaaaaga ugaucgagcg ccagaugaac     2820

gauaccagau acauuucuaa auauaucagc ggcguucucu ccaauauugu gcgggucgaa     2880

gacgggacgg acgagggagu gaacagcaag aauaucguuc cgggcaacgg gaagaucacc     2940

acacaacuga aacaggauug gggacuuaau gacgugugga acgaucugau ucucccucgc     3000

uucgagagaa ugaaucaccu gaccaacucu acagacuuua ccgccuggaa ugaaaaccac     3060

cagaaguucc ugcccacggu cccuguggag uuuuccaaag gcuucagcaa gaaacggauc     3120

gaucaucgcc accaugcucu ugacgcccug gugauugcgu gugcuacaaa ggaucacguu     3180

aaucuccuga acaaccaguc cgccaaaucu gacaccaaga gauacgaucu caagaaaaag     3240

cugaugaaau uugaaaaggu cgccuacaau cacccccaaa caggggagcg gaucgaaaga     3300

gaggcuccua aacaguuccu uaagcccugg gagaccuuua ccauugacgc caagaaccau     3360

cuggaaacaa ucaucgugag cuucaaacag aaucuccgcg ugauuaacaa ggcuacgaau     3420

uauuacgaga aauaugucga aaaggagggc accaaaguga aggaacgggu ugagcagaag     3480

ggaacaaacu gggccaucag aaaaccgaug cacaaggaga ccguguccgg caaaauugau     3540

cugccucgca ucaagguccc caaagggaag auucugacag cgacccggaa gucucuugac     3600

acgagcuuca aucugaaaac aaucggaucc auuaccgaca ccggcaucca aaagauccuc     3660

aaaaacuacc ugcaguauaa ggauaauaac cccgaacucg ccuuuucucc ugaggggauu     3720

gaagacauga auaaaaacau cgagaaguuc aauaacggaa aagcucauca gagcauuucc     3780

aaggugagag uuuuugagcu gggcagcaag uucccgcuug ggcaaucugg caauaaaaag     3840

gaaaaauacg uggaggccgc uaagggaaca aaccuguuuu ucgccaucua ucaggauaaa     3900

aauggcaagc gcuccuacga aaccauuccc cucaacgagg ucaucgaacg gcagaagcag     3960

gggcugagcg ugguugaccu gaaaggagug gaugacuuuu accuuucucc uaacgaucug     4020

gucuaugugc ccuccgagga cgagcucgaa aauaucagca ugauugauuu caacaagaug     4080

ucuuccgaga gaaucuacaa aguggucagc uuuacaggca aucaaauuuu cuuuguucgc     4140

caggacaucg cgacguccgu ggugaacaag gccgaguuca gcacccugaa uaaaauggaa     4200

cgggcuauug augggacaau gaucaaggaa accugcguca agcuccagau cgacagacug     4260

ggaaacauuu cuaaaaucug a                                               4281


<210>  4496
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4496
auguacaaca ucggccugga cauugggacc ggaagcgugg gcuggugccu cacagaugag       60

aauggguauc ugcuuaaggu caacagaaaa ggaaauaacg gcaauacgua ccggaacucc      120

gccuggggag uucgccuguu cgaaucugcu gacaccgccg cggauuguag aaucaagcgg      180

agcacacgca gacgguauaa acgcagacgg acccgcauua ucgagcucag aaagauuaug      240

uccgacauga ucaugcccau cgauccuaau uuuuacgcuc ggcuggacga agccuuccuu      300

uggaacgagg auaaaucuga caaggccaag gcuccguuuc uccuguucaa ugauaacggg      360

uaugacgaug ugaaauacua uacagacuac cccaccauuu accaccuccg caagcaucug      420

cuggaaacaa aagagaaggc cgauccuaga uuuaucuauc uggcccuuca ccauaugaug      480

aaauaccggg gccacuuccu guuugagggc cagagcuucg aagcuauuga caauaucgag      540

gacaccuuua uugaacucga gcaucuggug aacgucuaug ugaaggagaa ggaagauacg      600

gacaauaacg cggagaauaa cgcccuuuac caagaaauga aaaacuaucu ggcugauaau      660

aagguuaaaa acaaggacaa aaaggaguac aucaccgaua cauucauuaa agccgacuau      720

gauaauaagu acuccaagga acucgcugcc gcggugcugg gguacgaguu uaacgucgga      780

aucaucguga augacaacuc ucugaccgau gaggacggca aagcccucaa ggcuaaauuc      840

gccgaugcua aguaugaaga gaaagaagag aagcugagcg acacacuugg ggaacgcuac      900

uauauuaucg agacccugaa gaaaauuuac uccuggaagg uucuccacag cauccuggga      960

gacaauaaau aucuuucuua cgccauggug gauaaguaug agaaacacuc cgaacagcug     1020

aaggcccuca aguaccuguu ucauaaauac acgagccagg acgaguauuc ugaguucuuc     1080

caccaggaaa agaacaaaga aggcaaguac auugucaauu augcuaacua caucaaaggg     1140

aucaagagac uguccaauga gacaaacaag aaauauaaua ccaagcaaca gcucuaccag     1200

agcauuauga aaauccuggg agagcgggcg gccgaugacg aaguguauaa gaaaauucuu     1260

guggagaugg aacaggagac auuucuggag aagaucaaca augucgauaa cucugcuauu     1320

cccuaccaac ucaaccugau ggaaauggac aaaauccuua cccagcaggg cguuuacuau     1380

aaggagcuga gagauaauaa ggaacuccug cugaaaaugc ucaccuccaa gaucccuuac     1440

uaugugggcc cccugaacaa uaacagcaau gggaaccgca auuucgccug gaugacgaaa     1500

aaggacggaa aagagaacga aaagguguac ccuuggaaug ucaaggaugu gguugacauu     1560

gaugugacag ccgaggacuu uaucacccgg augacaaacu auugcaccua ccuuccgaau     1620

gagaaagucc ugcccaagga aucccuccug uaucagagau acaugcugcu ugaggaacuc     1680

ucucaaauuc gcaucgaugg ccggaaacug agcaaggagg acagaaaagc uauuaucaac     1740

gaccuguuca uugggaaggg acgcgugaag guuuccgaua aagacuuuaa ggaauaccuc     1800

aauaaaguga acuaugucaa ggugaauggc aaaggguacg augucacagg auaucagagc     1860

gacgauggcu ucgccugugc gcugucuucc uacaacaagu uucggagaau ccuuggccau     1920

aucgacgagc agaacgagaa gaugauugaa gauaucauuu auuggaucac cguguucgag     1980

gacaaagaua uuguuaagcg caaaaucaau aagcaauacg cugaccggcu gagcgccgaa     2040

cagcucaaaa agauccugaa acugaaguuu aagggguggg gaagauauuc uagcaaacuu     2100

cucaacggca uuaaggggga uaugggaacg acaaucauug agaugcugga agacgccgau     2160

gagcgcuucg cuuacugcga cuacuguccu aauuuuaugc agaucauuaa caaagacgag     2220

aagaucaaac agaucauuga ugacaaucgg cccagauaug auggcaccaa ggaccugcuc     2280

gaugugaucc aagacaugca caccuccccu gccaaccggc gcgggauuug gcagacaaug     2340

aaggcuaucg aagagauuau cgaauacaug ggcgagaaac cccagcagau cuauauugag     2400

uucgccagag aagaugaccc gaaggcgaaa aauaagcggg ccgauucuag aaaacgcgcu     2460

aucgacaagg cccugaagaa acuuaaggag gaugucgugg acgaauacaa cgagaaaguu     2520

uauaaggaac ugaaacaaua cgagaagcgg cucgacgagg aaaaggugua ucuguacuuu     2580

augcagaaug gaaaagaucu guacuucccu gacaaggagc uuaaccucga ugaacuggag     2640

aaacuggaaa uugaccacau cauucccuau agccucuccg augacgauag ccuggacaau     2700

aaggcucuug uccugaaagg caacaaucag aacaagggga auaagaucgu gaaagaggcc     2760

uucccucagc ucuuuucuaa cuccggaacc gugaacuacu ggaagaaucu gaaaaaggcg     2820

ggccugauua gcgagaaaaa guauaacaau cuucagaaaa acaauguuga ugacauccuc     2880

acaaaggggu ucaucaacag acaacugguc gaaacgcgcc agauugugaa gucuguggcc     2940

aaucugaucc ucgauuacua uaacgagcag auugacguca ucgaagugaa ggcuucccug     3000

agcaccuccg uuagaaaaau gcuuacauac gagaaaaagg auaauaacgg auuuuggauu     3060

gaaaauaaag acaacugcau guucuauaag aaucgggaca ugaacgauua ccaucacgcc     3120

caugacgcuu accuggccaa uaucaucggc cucuauauuc aaaagaacua cccccgccug     3180

cagaaagagc ugaauuauuc ucaguacaga cggaucuggc gcaaguauua cgagaacacc     3240

aaaaacaaua acggggugaa uugguuugcc acacuuggca aguucagcuc caacaaugaa     3300

gaggcugauu gguauggaca agacauuauc gcguacauga gaaaaaucuu uuguuaccgg     3360

gauguccuga uuucuaagaa gguggaagag aacaccggcg ccuucuauag cgagacgaaa     3420

uacccccgcg aagacaaggc uggguccaaa cucguuccuc ugaagcaggg aaauaacaug     3480

agaggcgcca auaaccucaa agagcuggau gcccggaagu augggggaua caagggcggg     3540

gaaaaggcuu auuuugugcu ugucaaauac ugcaucgaga aagcccugaa gaaaaaggug     3600

aaaaaggaau aucacaugga guucguugag auuccggugu acaucgcgcg cggcauuaag     3660

aauaaagaca ucaaccucua cgauuauguc ugugacauuc ugaaugaagc uaacaagaau     3720

agcguggccg auguggagau ccugagaaac aaagucccca aguaccagau gauuaucgga     3780

gaaaacggcg aggaauauua ccuuguuucu gccaccgagg ugaucaauuc caaacaguuu     3840

gugcucgggg gagcuaacca acaguauaau cggcugcuga acuacauuaa guauggcaag     3900

aaugacaaau ggcaguacau ccagacagag cuccuggaug aacaacuuau ugggcuguac     3960

aaccuccuga ucgccaagau uaaagacgag uauaagggau ucagcaaaga agcggucaga     4020

auccaggaga auaacucuuu ugacaagcug gaugugaagg acaaaaagga guucaucgcu     4080

gaaaugauua aacuuguuca gccugauucc gacuaccccu aucugggcaa guacgccacc     4140

gggcucagca aucgcauggg ccggaaaaca ggagagaagg ugggcaagaa aaucacccug     4200

gucgauaagu cugugacggg gcucuaugaa agacgcacaa ccuucgagcu ggaagacgau     4260

agcuccacaa aaagcugcug a                                               4281


<210>  4497
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4497
augaagaaca uccugggccu cgaccugggg accacaagca uuggauucgc ccacaucgug       60

gaggaugaaa auaaagagaa guccgaaauu aaagagcuug gcgucagaau cguuucucug      120

acgaccgacg aacaaagcga uuuugagaag gggaaaucca uuacaaccaa cgcuaaucgg      180

acacucaagc auggagcccg ccugaaccuu gacagauacc aacagcggcg caaguaucug      240

aucgaucucc ugcagaaagc gaaucugauc acccccucua gcauucucgc ugagaacggc      300

aagaauacaa cccacuccac guggcagcug agagccaaag ccgugaccga acggaucgag      360

aaggaagagu ucgcucgcau ucuucuggcc aucaacaaaa agagagggua caagucuagc      420

cggaaagcua agacagagga cgaaggacaa gccauugaug gcauggcgau cgccaaacgc      480

cucuaugacg agaaucugac cccuggccag cuuucccugc agcuccugca gcaaaacaag      540

aaacugcucc cggauuuuua cagaagcgac cugcagaagg aguucgaucu uguguggaau      600

uuucagaaac aguucuaucc cgacauucug acagauaucu uuuacaagga gcuccaaggg      660

aagggaaaag acgcuaccuc uaaggccuuc uccaaacggu aucauuuuga cacgacagaa      720

aacaagggca gcaaagaguc uguccgccug caggcuuacc aguggagagc cgaggcgauc      780

uccaagcaac uuagcaagga agagguggcc uacguucuga ccgaaauuaa uaacaaucuc      840

aacaacgcuu cuggguaucu gggagccauc uccgaucgga gcaaagagcu guacuucaau      900

cgccagacag ugggccagua ucucuacgcu aagcugcagg aaaacagaca caauucccuu      960

aaaaacaagg ucuuuuaucg gcaagacuac cuggaugagu ucgagagaau uugggaaacc     1020

caggccucuu uucauaaaga gcucaccgac gaacugaaga agcagauccg cgaugugguu     1080

auuuucuauc agcggaaacc uaagagccaa aaagggcuua ucuccuucug cgaguuugaa     1140

agcaaggaga ucgagauuga aaaagacgga aagacaauca cgaagaauau uggcgccaga     1200

guggucccca aaucuucccc ucuguuccag gaguuuaaga ucuggcagau ucucaacaau     1260

gugaucugua aacgcaaggg cauccggaaa aagaaaauua gcgcuaagac cacacagcug     1320

gaucugcuca acgaaucuag ccaaaccauc uucucccugg acauggagug caagcagcuu     1380

cuguuugaug agcucaaucu gaaaggggac cugaagucug auaaagugcu uaagcuccug     1440

ggauacagcc cccaggaaug ggagauuaac uacaaucagc uggaaggcaa cagaacacaa     1500

aaagcgcucu augaggccua ccugaagauc gucgaaaugg aggcucacga cguuaaggac     1560

auucuucaga ucaaauccgc caaggaugac uggagccugg augagucucc ucucuccgcc     1620

agcgaaauuc gcgagaaagu gaaggcuauc uuccagaccc uggggaucug uacgaaaauu     1680

cuguauuuug acccgcuucu ccccgugaag gaguucgagg aacaagauuc uuaccagcug     1740

uggcaucugc ucuauuccua cgagagcgac gauuccacau cuggaaauga gacccuguau     1800

cggauccuug aaaagaaaua cgccuuuaag agagagcacg cgcgcauucu ggcuaacguc     1860

gcccuccagg acgauuacgg cagccugucc accaaagcca uccggaagau uuauccuaau     1920

aucaaagaaa accaguacuc uacagcuugc gagaaggccg gguauaagca cagcaaacug     1980

ucccuuacca cagaagaacu cgaggcuaga gaacugaaga acaucauucc ccugcucaaa     2040

aagaaugccc ugcgcaaccc ugugguugag aaaauccuua aucaaaugau uaacgugguc     2100

aaugcgcuga ucgaaaagaa cagcgagcgg gacgccgagg gaaaaauuac gaaguacuuc     2160

cauuuugaug aaaucagaau cgagcucgcu cgggaacuga agaaaaaugc ccagaagcgc     2220

uaugagauga cccagaacau uaauaaagcu aagcuggaac accagaaaau cucugagauu     2280

cuucaaaagg aguucggcau caagaacccc acaaaauccg acauuaucag auaccggcuc     2340

uaucaggaac uggagcacaa uggcuacaag gaacuguaca ccaacgcccc gaucgcgaga     2400

gauaugcucu uuagcaaaaa uauugagauc gaacauauug ugccuaaggc ccgcguuuuc     2460

gacgacucuu ucuccaacaa aacacugacc uuucaccgga ucaauagcga uaagggggag     2520

uauacggcuu ucgacuacau uaccucucuu aacagcgagg aagaacugaa ccaguaucuc     2580

acaagagugg aaaaugccua caagaccaaa uccaucagcc ccacaaagua uaaaaaccug     2640

cugaagaagg cuucugagau uggagaugac uuuaucaauc gcgaucuuag agacacccag     2700

uacaucgcca aaaaggccaa agaaauucug uuccaaguca cgaagaacgu gcucuccaca     2760

agcggcucca ucaccgaucg gcugcgcgag gacugggggc ucgucgaugu gaugaaagag     2820

cugaauaugc cuaaguauca gucucuugga cugacagaag uugaggaaag aaaagacggc     2880

aacaagguga ccgucauuaa gaauuggacc aaacggaacg aucaucgcca ccaugcuaug     2940

gacgcgcuca caguggccuu uacgaagccc agcuacaucc aguaccugaa ucaccugaac     3000

gcuagaaaag augagaauaa caagaauuau uccguuauuc uugccaucga ggaaaaagag     3060

accaucaagg ugcccacaaa caaugggaaa aacaagcggg ucuucauuga accuaucccg     3120

aacuuucgcc aaguggccaa gaaacaccug gaggaaauuu ucaucucuca uaaggcuaaa     3180

aauaaggugg uuaccaaaaa cacaaauaag cccgccggca ccgacaagca gcagauuacg     3240

cucacaccua gaggacagcu gcacaaagag accaucuacg gcaaguauca auacuauauc     3300

aacaaagagg aaaagauugg ggucaaauuu gacgagcgga ccaucgcgaa ggugagcaau     3360

cccguguacc gcgaagcucu ccugaagaga cuucaggcca acgauaauga cccuaaaaag     3420

gccuucgcug gcaaaaacgc ccuguccaag aaucccauuu aucucgauga gucuaaaaca     3480

aagacccugc cggaaaaggu caaccugaca uaccuugagg aggacuuuag cauccggaaa     3540

gauauuuccc cugacaauuu caaggaucug aaaagcaucg aaaaggugau ugaccaggga     3600

guuaaaagaa uccucaucaa gcgccugcag gcguacgaua acgaccccaa aaaggcuuuu     3660

guggaucucg agaagaaucc uauuuggcug aacaaagaaa aggggaucgc cauuaaacgg     3720

gucacgaucu cuggagugaa uaacgcccaa ccccuucaua uuggcaagga ccaccugggg     3780

aaaaccacac ugaauaagga gggcaaggaa aucccuguug acuauguguc caccggaaac     3840

aaccaccaug ucgcuaucua cagagauaaa gagggcaauc uccaggagca gauugugagc     3900

uucuucgacg ccguugugcg cgcucagcaa gggaucccca uuaucgauaa gacauauaaa     3960

caggccgaag gauggcaguu ucuguucacc augaagcaga acgagauguu ugucuucccg     4020

aaugcgacga ccggcuuuaa cccugccgaa auugaccuuc uggaucccaa aaauaagaag     4080

cucaucucuc ccaaccuguu ccgggugcag aaaaucgcua caaaggacua cuuuuucaga     4140

caccaucucg agaccaaugu ggagacagau aacauucuga aaaaugucac cuggaagcgc     4200

gaagggcuuu ccggacugaa agacaucguu aaggugcgga uuaaccaccu gggcgauauc     4260

gugagcauug gggaguauug a                                               4281


<210>  4498
<211>  4281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4498
augaagaaca uccugggccu cgaccugggg accacaagca uuggauucac gcacaucgug       60

gaggaugaaa auaaagagaa guccgaaauu aaagagcuug gcgucagaau cguuucucug      120

accacagacg aacagagcga uuuugagaag gggaaaucca uuucuaccaa cgccaaucgg      180

acacucaagc auggagcucg ccugaaccuu gacagauacc aacagcggcg caaguuccug      240

aucgaucucc ugcagaaagc caaucugauc accagcuccu cuauucucgc ggagaacggc      300

aagaauacaa cccacagcac guggcagcug agagcuaaag ccgugaccga acggaucgag      360

aaggaagagu uugcccgcgu gcuucuggcu auuaacaaaa agagagggua uaaguccagc      420

cggaaagcca agacagagga cgaaggacaa gcuaucgaug gcauggccau ugcgaaacgc      480

cucuacgacg agaaucugac ccccggccag cuuucucugc agcuccugca gcaaaacaag      540

aaacugcucc cugauuucua uagauccgac cugcagaagg aauuugaucu ugucuggaau      600

uuccagaaac aguuuuaccc ggacauccug acagauagcu ucuauaagga gcuccaaggg      660

aagggaaaag acgccaccuc uaaggcuuuu uccaaacggu accauuucga cacggccgaa      720

aacaagggca gccgcgaguc ugugagacug caggcuuauc aguggcgggc cgaggcgauu      780

uccaaacaac uuagcaagga agagguugcc uacgugcuga cagaaaucaa uaacaaucuc      840

aacaacgcuu ccggguaccu gggagccauc ucugaucgca gcaaggagcu guauuuuaau      900

agacagaccg ucggccagua ccucuaugcu cagcugcaag aaaacaaaca caauucccuu      960

aagaacaaag uguucuaccg gcaggacuau cuggaugagu uugagagaau uugggaaaca     1020

caggccagcu uccauaagga gcucaccgag gaacugaaaa agcagauccg cgacguugug     1080

auuuucuacc aacggaaacu uaaaucucag aaggggcuga ucuccuuuug cgaguucgaa     1140

agcaaagaga uugaaaucga gaaggaugga aaaaccauca caaagucuau uggcgccaga     1200

gucgugccca agagcucccc ucucuuucag gaguucaaaa ucuggcagau ucugaauaac     1260

gugacgugua agcgcaaagg cauccggaag aaaaagucua gcgcuaaauc caccgaacug     1320

gaccucuuua augagcccag ccaaacaauu uucucucugg auauggaaug caagcagcuu     1380

cuguuugagg aacucaaccu gaagggggac cugaaauccg auaagguccu uaaacuccug     1440

ggauauagcc cucaggagug ggaaaucaau uacaaccagc uggagggcaa uagaacccaa     1500

aaggcgcucu acgaggccua ucugaaaauc guugaaaugg aggcucacga cgugaaggau     1560

auucuucaga ucaagucuac aaaagacgac ugggcccugg aagagagccc ccucuccgcc     1620

uccgaaauuc gcgagcgggu gaaggcuauc uuccagaccc uggggauuug uacgaaaauc     1680

cuguacuuug auccucuucu cccggucaag gaguucgagg aacaagacuc uuaucagcug     1740

uggcaucugc ucuacagcua ugaauccgau gacucuacaa gcggaaacga gacccuguac     1800

cgcauucuug agaaaaagua ugccuuuaag agagaacacg cgaccauccu ggcuaaugug     1860

acacuccagg augacuacgg cucccugagc accaaagcca uccggaagau uuaccccuau     1920

aucaaagaga accaguacuc uacagccugc gaaaaggcug gguauaaaca cuccaagcug     1980

agccuuacga ccgaggaacu cgaggccaga gagcugaaga auauuauccc ucugcucaaa     2040

aagaacgcuc ugcgcaaccc cguuguggag aaaauucuua aucaaaugau caacgucgug     2100

aaugcccuga ucgaaaagaa cucugaacgg gaugcggagg gaaaaauuac aaaguacuuc     2160

cauuuugacg aaaucagaau ugagcucgcc cgggagcuga aaaagaaugc ucaggaacgc     2220

uaugagauga cccagaacau caauaaggcc aaacuggaac accagaagau uuccggcauc     2280

cuucaaaaag aguucggcau caagaacccu acaaaaagcg auauuaccag auaccggcuc     2340

uaucaggaac uggcucacaa uggguacaac gaccuguaca cgaauaccua uauccccaag     2400

gagcuccugu ucucuaagaa cauugauauc gagcauauug uuagcaaagc cagaguguuu     2460

gacgauuccu ucagcaauaa gacacuugcg uuucgcgagu ucaacaucaa aaagggagaa     2520

ucuaccgccu uugacuacau cacaucccug aauaacgaag aggaacucaa ccaguaucug     2580

aaucgggucg agaacgcuua caaaaccaag aauauuagcc cggccaagua uaaaaaccug     2640

cuuaagaagg cuuccgagau cggcgacggg uucauuaaua gagaucugcg cgacacgcag     2700

uacaucgcca agaaagccaa ggaaauucuc uuucaaguga caaagaacgu ccugucuacc     2760

agcggaucca ucacagauag acuccgggag gacuggggcc ugguggaugu uaugaaagaa     2820

cuuaauaugc cuaaguauca gucucugggg cucaccgagg uggaggaacg caaagagggc     2880

aacaagguca ccgugauuaa aaauuggaca aagagaaacg accaccggca ucacgcuaug     2940

gaugcgcuga cgguugccuu caccaagccc agcuacaucc aguaccugaa ucaucuuaac     3000

gcucgcaaag acaagaauaa caaaaauuau uccgugaucc uggccauuga agagaaggaa     3060

acaaucaaag ucccuaccaa caacggaaag aauaaaagag uguuuauuga gcccaucccc     3120

aacuuccggc aaguggccaa gaagcaccuc gaggaaauuu uuaucucuca caaagcuaag     3180

aauaaaguug ucacaaagaa caccaauaaa ccugccggca cggauaagca gcagaucaca     3240

cugaccccgc gcgggcagcu ccauaaggag accguguacg gaaaauauca auacuaugug     3300

aacaaggaag agaaaauugg cgucaaguuc gacaaagaaa caaucgcgaa ggugagcaau     3360

cccguuuaca gagaggcucu gcuuaagcgg cugcaggcca acgauaauga cccuaaaaag     3420

gccuuugcug ggaaaaacgc ccucuccaag aaucccauuu aucuggauga ggcgaaaacc     3480

aagacacuga cggaaaaggu gaaccuuacc uaccuggagg aagacuucag cauccgcaaa     3540

gacauuucuc cugauaauuu caaggaccuc aaauccaucg agaaggucau cgaucaggga     3600

gugaaacgga uucugaucaa gagacuccag gacuacgaua acgaccccaa aaaggcuuuu     3660

guugaucugg aaaagaaucc gauuuggcuu aacaaagaga agggcaucgc cauuaaaaga     3720

gugacaauca gcggggucaa uaacgcccaa ccucugcaca uuggcaagga ccaucuggga     3780

aaaaccacac ucaacaagga gggcaaggaa auccccgugg auuauguuuc uaccgggaau     3840

aaccaccacg uggcuaucua ccgcgacaaa gagggaaauc ugcaggaaca gauugucucc     3900

uucuuugagg ccgugguggg cgcucagcaa gggcuuccua ucgucgauaa gacguauaaa     3960

caggccgagg gauggcaguu ccuguuuacc augaagcaga acgaaauguu cguuuuuccc     4020

aaugaggcga caggcuucaa cccugccgaa auugaccucc uggaccccaa aaauaagaag     4080

caaaucagcc cgaaccucuu ucgggugcag aaaauugcua ccaaggauua cuucuuuaga     4140

caucaccugg agacaaaugu ggacaccgau aacguccuua aaaauacaac cuggaagcgc     4200

gaagggcugu cuggccugaa agacaucgug aagguucgga ucaaccaucu cggagauauu     4260

guguccaucg gcgaguauug a                                               4281


<210>  4499
<211>  4284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4499
augaagaaaa uccugggccu cgaccugggg accagcucca uuggaugggc ccuugugaac       60

gaggcugaaa caucugagga aaagagcucc aucauuaaac ugggcgucag aguuaauccc      120

cucacggugg augagcugca gaacuucgaa aaggggaaau cuaucaccac aaaugccgac      180

cggaccgcgg gacgcagcau gagacggaac cuucaacgcu acaagcugag acgggagaau      240

cucauugagg ugcugaagga auauaacuuu aucacagaug agaugauccu guccgaaaau      300

ggcaaccgca ccacauucga gaccuacaga cuccgggcua aagccgccgu cgaggaaauu      360

ucucuggagc aguuugcucg cgugcuucug augaucaaua agaaaagagg guauaagagc      420

ucccggaaag ccaagggagc ugaaaacggc acgcucauug acggcaugga gguugccaag      480

aaaauguacg augaagggau caccccugga gagcugugcc uucagcugcu cacagcgggc      540

aagcgcuauu ucccggacuu uuacagaagc gaucugcagg ccgaguucga ccggauuugg      600

aauuuucaaa aacaguucua ucccgauucu cugaucgaca aggugaaaga ugaaguccgc      660

gggaagaaca aaucccagac cugggcuauu cucgccaagu acuuugugug gaaggagguu      720

gaaaauagcu ggaacgagga agaggcucag acaagacggg uggagaaaga auacagacug      780

gucggaauca agcgcggcgu gaaacgggag gaacuuaagc uggagaauuu ccaauggaga      840

gugaaagccc ucucugaacg caugaacccu gaggagcugg cgaucguccu ucaggaaauu      900

aacgagcaga ucuccaauag cucuggguau cugggagcca uuuccgaccg gagcaaggaa      960

cucuacuuua acagacagac cguuggccaa uaucagaugg cugugcugga uaauaacccc     1020

aauaucggcc ugcgcaacau gguguucuac cggcaggacu aucucgacga guuuaauacg     1080

auuugggaga agcaagccga guuccacaaa gagcugacag aagaucuuaa gaaagagauc     1140

agagacaucg ucauuuucua ccagcgccgg cugaaguccc agaaagggcu caucaacauu     1200

ugugaauuug agagacgcca gaucgaggug gaaauugaug gaaagaagaa aaucaagacc     1260

aucggcucuc ggguuauucc uagaagcagc ccccuguucc aagaguuuaa aaucuggcag     1320

acacuuaaug acauugaagu gagcguccug ggggugaaga acaaacggaa gaagcaggau     1380

gacaauucua ccacccuccu ggauuccgcu gagaacaucg acagccugaa acucaauguu     1440

ucucgcccuc uggaugccga cgaaaagagc cuucuggcca aagagcuuuu cauuagagau     1500

aagcugacaa aauccgacgu gcugaagcuc cucuuuaaca auccgcagaa ccugaaccug     1560

aauuucaaaa acaucgacgg aaaucggacg ggcucugcuc ucuaucaagc guuuagcaag     1620

auucuggaga ucuccgggca ugaaagcauc aacuucaaga aaucugccga ugagauuguc     1680

gaacagguga agaccaucuu uuccgcucuu ggauggaaua cagagguccu gugcuucgac     1740

agcgaaaaag agcucgauaa gcagcccuac uuuaaacugu ggcaucugcu uuacucuuuc     1800

gagggcgacu ccaccccuac aggcgauggg aaccucauuc agaagaucgc cgaccugugu     1860

ggauuugaaa aggauuaugc cagcauucug gcuaauauca ccuuccaaga ggacuacggc     1920

ucccucucua cgaaagccau cagaaagauu cugccccacc uuaaagaugg gaaccaguau     1980

gacguggcgu gcgaauacgc uggauauaag cacagcaaau ccucucugaa gaaggaggag     2040

aucaaaaaua agguucucaa agauaagcug gaacugcuuc cuaaaaacag ccuccgcaau     2100

cccguggucg agaagauucu gaaccagaug gugaauguua ucaacgccau uaucacaacc     2160

uacggcaagc cggacgaaau ccggauugag cuggccagag aacucaaaaa gaaugcuaaa     2220

gagcgcgagg aacugaccaa guccaucgug gauacaacca aaacacauga ggggauuaga     2280

gccauccuuc agaacgacuu cggcaugagc cacgucucuc ggaacgcuau uauccgcuau     2340

aagcuguacg aagaacucaa agacaaugga uacaagacgc uguauuccaa caccuacauc     2400

ccuaaggaaa aacuguuuag caaggauuuc gacauugagc acaucauucc ccaagccaga     2460

cuuuuugaug acucuuucuc caauaaaaca cucgagguga aguccaucaa cauugaaaaa     2520

ggcucuaaga ccgcguauga uuuuguggag gaaaaguacg gggagcaggg acugcaggaa     2580

uaucugaauc ggugugaggu ucucuuccgc gacaaaaaga caaaacugag aaaacuuaag     2640

augcaagaga gcgaaauccc ugagggcuuu auugaucggg accugcgcaa cacccaguac     2700

aucgccaaga aggcucucuc caugcugaau gaaaucugca gacgggucgu ggccacgacc     2760

ggggcuauua cagaugagcu gcgcgaggac uggcagcuug uggaugucau gaaagaacug     2820

aacugggaga aguauaaagu gcucggacug guugaauacu ucgaggacaa ugauggcaga     2880

cucaucgggc ggauuaagga cuggaccaaa agaaacgacc aucgccacca ugccauggau     2940

gcgcugacag uggccuuuac caaggacguc uucauccagu acuuuaauaa caagaaugcu     3000

agccuuaaac ccgauacgaa cgaagccgcu auucggaaua aguauuucca aaacggcaaa     3060

gccauucccc cuaugucucu ggacgaguuu agagccgagg cuaagaaaca ccucaacgau     3120

acacugaucu ccaucaagac caagaauaaa gugguuacaa ucaaccugaa ucgcaccaag     3180

aaaaagaacg gaauuaauaa aaagcagcag cagaccccgc ggggccaacu ucaccuggaa     3240

acaguguacg ggagccauaa acaguauguc gugaaggagg aaaagguuaa cgcguccuuc     3300

gacgccgcua aaaucgccac ggugaauaag cccgccuaca gagaugcucu ccugcgccgg     3360

cuccaggaga acggaaauga cucuaaaaag gccuucaccg gcaaaaacag ccuggauaag     3420

cagccugucu ggcuugacgc gaagcaaaau gaaugugugc ccgagaaagu gaagacaguc     3480

augcuggaga ccguuuauac agugagaaaa ccuauugaug aaaagcucaa cguggacaaa     3540

gucguggacg cucgcauccg gaagcugcug caggauagaa ucaaggagua cgggaaugcc     3600

aaauccgccu uugcuaaccu ugacgaaaau cccauuuggc ugaacaagga gaaaggaauc     3660

accauuaagc ggguuucugu gagcggcauc uccaaagccc agucucucca cgaaaagaag     3720

gauaaagacg ggaauuucau ucuggauaag gacagccgca gaaucccggu cgauuuugug     3780

aacacgggca acaaucauca cguugcgauu uaucggaaac cugugcucga caagaaagga     3840

caagcugucu ucgaugagaa cggcgagcug aaguuugaac uugaggaagu gguugugucc     3900

uucuuugacg ccgucacacg cgugaaucug agccagccca ucaucgauaa agacuacaag     3960

agaucugagg gguggaaguu ccuguuuucc augaaacaga acgaguacuu cguguuuccu     4020

aaugaaaaga ccggauucga ucccaaagag auugaccucc ugaacccuga aaauuaugcc     4080

cuuaucagcc ccaaccuguu ccggguccag aagauugcuu cuaaagacua cguuuuuaga     4140

caucaccucg agaccguggu ggccgaucgc aaggaacugc aagagaucac auggaagcgg     4200

auuuccagcc ucucuauccu gaauagaauc auuaaagucc gcgugaacca caucggccag     4260

auuguuauga ucggggagua uuga                                            4284


<210>  4500
<211>  4284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4500
auggccaagc ccaaagacua caccaucggc cuggauauug ggacaaacag cgugggaugg       60

gucguuacgg acgaucagaa uaacauccuc agaauuaagg gcaaaaaggc uaucggggcc      120

cggcuguucg cggagggaaa aguggcugcc gaacgcagau ccuuucggac cacacgcaga      180

cggcuuucuc gcagacggug gcgcauuaag cugcucgagg aacuguucga caaggagauc      240

gccaaagugg auccuagcuu uuucgcuaga cuucacgaau ccuggaucuc uccgaaggac      300

aaacggaagc gcuauagcgc cauugucuuu cccuccccug aggaggauaa aaaguuccau      360

gaaucuuacc ccaccaucua ucaccugaga gacaagcuca ugaaagauga ccaaaagcau      420

gauauucggg agaucuacau ugcugugcac cagaugauca aagcccgcgg caauuuucug      480

caugacgaaa gcguugagac auauagaucc gggaugagcu cucugggagg ccgguccgag      540

agaaccauuc ucagcgugca gacacuggag gaacuuaacg aucuguucgc ggaaaaugag      600

ggcaccgaag aggccaagcu caacgucgcu ucugccgagc agaucaauga cauccugaca      660

gggggacacc uuaacgcuga cucccaaaaa gaaauuggca aucugcuccu gccuagcucu      720

uuucccuccu ucgaugacaa ggccaaagag aaacagguga agaaacugau caagaacguu      780

gcgaccaaua uuagcaaagc cuggcucggg uacaaggcug auuuuuccac aauccugaac      840

cuugccaaug uggacaaaga ucagaagaag auuuucgcuu uugcccugca gggaggcgac      900

gaagaggaua aaguccaaga acucgagucu cugcuugagc agagccagac cgacaucgug      960

gaucgccuga ucgaaauucg gcacgccauc gugcucuccg agauuguccc uguugggaug     1020

acacugagcg aagcuaugau cgacaaguau gaucagcaua aagaggaccu gauuacccuc     1080

aaggcgguga ucagaaacac gaaagacaag aagaaagccg cuaagcugca agccaucuac     1140

gaucuuuacg ugaauaaccg ccacgccgac cuggcuaaag ccaugaagcu cacaggaauu     1200

aaaaagcggu cugaacugcu ugauccggag gagcugaaga aagcgaucuc cagccuccug     1260

gacgauucuc ccgaagcugu cgagauuaag cagaugcugg aagagaaaac cuuccucccu     1320

cugcagagaa gcaacaauaa cggcgugauc cccaaucaac uucaucaggu ugagcuggac     1380

gaaauuauca agaaacaguc caaguauuac ccuuuccucg ccgagaaaaa ccccgaugaa     1440

ucugaggaag cccagaagaa ggcuccgaca aaacuggacg cccugcuuac cuuucgcgug     1500

ccuuauuacg ucggccccau gauuaccaag gaggagcaag aagcucagga ugggcacagc     1560

uucgccugga uggugcggag agacccucag gaucacgagg cgaucacacc cuggaauuuu     1620

gaaaagaagg uugacaaaau ggccuccgcu acgcaguuca ucaagcgcau gaccacaaag     1680

gauaccuauc uccugggaga ggacgugcug cccgccagcu cucucaaaua ccaacuguuu     1740

acaguccuua acgaacugaa uaaccuccgg gugaauggca agaaacugac cuccgacgag     1800

aaggagcagg ucauugaagg gcuguucaaa aagcagaaga cggugaaagc ugauaaguuu     1860

guuaaauauu ggcaggccaa gcauaucgga gcggacauua aagugaaggg ccuuagcgau     1920

ccuucuaagu ucaacuccac aaugagcacc uacaucgacu uuaaaaagau uuucggggau     1980

caacucaaug acgucaacag acagaaugau uuugagaaaa ucaucgaaua cuccaccauu     2040

uucgaggacc gcaagauccu ggaagauaaa cugcgggccg acuuugcuug gcucucugag     2100

gagcagauua agcagcugug cagacuucgg cugcaaggau ggggccgccu cagcgauaaa     2160

cugcugacaa gacuuaccga cgccgauggc cagaacgugc ucgaacggcu guggaauagc     2220

aacgacaacu ucguucagau cgugucugac ccgaauauua aggcuaagau cgagcaagaa     2280

aaccagaaac ugcucagaga ugggcagagc gacgaugccg ucgaguccau ccuggacgau     2340

gccuauacaa gcccccagaa uaagaaagcu auucgccaag ugaugcgggu gguucacgac     2400

aucgucaagg cgugugaugg aaacgugccu gccaaauucg cuauugaguu ugccagaucu     2460

caggaagacg aucccaagcg cacgaccgcc agagugaauc agaugcagaa gaucuacgac     2520

caaauuuccg augaggucgu gagccagggc guuaaagaac agcuuucugg gaugaagucc     2580

cugaaagacc gguauuaccu cuauuucaug cagggaggcc gcgacgcuua cacaggggcc     2640

aagcugaaca ucgauagacu gagcgacuau gauaucgacc auauuaugcc ucaaucuuuu     2700

gugaaagaug acagccuuga uaaucggguc cucguggcgc gcgcuguuaa caaucagaag     2760

uccgacaagg ugcccgcccu gcuguucggc aacaaagucg uggccgaucu cggaaucacc     2820

guuagagaga ugugggacaa guggcagaaa cugggcauga uuagcaagcg gaaacuuucu     2880

aaucugcuca cagauccgga cgcucugacc gaauaccgcg cccaaggguu uaucagacgg     2940

cagcuggugg agacguccca ggucauuaaa cuuaccgcga caauccugca gagcgaguuc     3000

ccugauucca agauuaucga agugcccgcu aaauacaacu cuaucgugcg caagcaauuu     3060

gaccucuaua aaagcagaga ggucaaugac uaccaccaug ccauugaugc cuaucugucc     3120

accaucguug gaaacuaccu cuaucaggug uacccuaauc ugcggagaau guucguguau     3180

ggcgaauuua agaaauucuc uagcaacgcu gaggaauccg ccuacgacgu cgugcgccgg     3240

guuaagucua ugaacuuucu ugaugaccug cucagaggga cacacggaga uaauauuuac     3300

ugccgcagca ccggcgagau cguguucaac cggaaugaca uuaucuccaa gcugaaacag     3360

gcuuauagcu uuaagcagau gcuggucacg caagaggugu ucacgaaaaa gucugcccuu     3420

uucgaucaga ccguuuaccc cuccccugaa agagacagca aaaagcgcuc ugggcugauc     3480

ccccggaaaa agggcaugga uacagagauu uauggaggcu acuccgggaa caaggacgcg     3540

uauuuugugc ucgccgaagc ugucaaagag aagggacaca cccugcagau cgugggcguu     3600

ccgauuagag cccucaauac ccugaaaaac agcgcuaauu acucugaaaa acuucuggag     3660

aucauuaagc cucaggugau guucaacaag gauacaggga agcccaucaa aggaaucaag     3720

gacgucaaaa uucucaugga uaagaucccc ugucgccaac cugugcugga gggcgaaucc     3780

uauuacaugc uggccagcuc caaauaccgg uauagccuua agcagauuuc ucugagccag     3840

auguccauga aguacauccu cgacuauauu gacgauccga auuuuaacaa acaugagaug     3900

aucaauauug accagcaaga ugaaaaggag ugccugcucu cuguguacga cgagauccug     3960

gaaaagaugg auaaguaucu uccccuguuc gacaucagau ccuuucggaa aaagcugcac     4020

gaugggcgcg acgccuucau ugcucucccu gucgcguccg aggaaaagaa acccggcaag     4080

guugauguga ucagaaaagu gcugaaggga cuucaugcca acgcugacau uacgaaucug     4140

gccgagcucg gcuuugggac cgccgcucug ggagcccucg ucucuacagg cgggaucaaa     4200

auuagcgaug acgcgguguu caucuaccag uccccuaccg gacuguuuga acggcgcguu     4260

aaggugucug accuucugaa auga                                            4284


<210>  4501
<211>  4287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4501
augaagaaaa uccugggccu cgaccugggg accacaagca uuggauccgc cuacgugcuu       60

gaggcugaaa agucugccga gcagacgaaa aucgucuauc ugggcaccag aauuauccuc      120

agcuccgcgg augcugaaaa cuucucuaag gggcugagcu gcucccccac aaccgaccgg      180

acauuuaaac gcucuaugag acggcgccuu caaagauaca agcagcggcg cagcucccug      240

auucagcagu ucaagaaaau cggaaucauu accaauacag agcaaaucau uagagaaccu      300

aucagcgaga cccacgagcu ccugcggauu cgcucuaagg ccguuuccga aagagugagc      360

cuggaggaau uugcccggau ccuccugacg auuaacaaac gcagaggcua uaagucuaau      420

cggaaaaccu ccaagagcac agaggaggau gggaaacuug uggacgcuau ggccgucacc      480

aaggaacuga ucaagcucaa aaagacaccg ggacaguaca uguucgagcg cuuuaaaaga      540

ggcaaguaug cugaaaucga uuucuacccc uuugaccuga uuaacgagcu uaaacagauc      600

ugggaagugc agggccaaaa guauccugau guucugaccg aggaccuccu gucuucccug      660

cucaagcaga aaacggggaa ggccaaagag cugauuuaca gcaaguccga ucuuaauccc      720

gcggaagugc cuaucgacua ccagaaagga aagaagcugc ggaaaacaau ucucaacaag      780

uauuucgccu acgagucuag aagccuggcu gccaccaaua ucauccccgu cgaacaggug      840

auuacagcua ucguugccau uuguaaacaa aucaacaauu ccagcggcua ucuuggggcg      900

auuggagauc gcucucgggu gcuguacgcc aacaaucaga ccaucggcca guaucucuac      960

gagagaauca aggcuaaccc ucaugaccgc auuagagggg aagucuuuua uagacaagau     1020

uaccuggacg aguucgaccg caucugggag ugccagcaga auuacuaucc ggccauucug     1080

accaacgauc ggaaacaguu uaucagaaau cgcauuaucu ucuaccaacg gccccucaag     1140

uccaagaaaa gcgaacuggc uuuuugugag uucgaaucua gagaggugga acacaacgga     1200

aagaaauaua cagagggccu ucauguggcc ccuaagagcu cccccgucuu ccagcacuuu     1260

cgccuguggc agcggcucgg ccagauugag guuucuccuc ugagaaaaug gaacaagcaa     1320

agcaaugaac uuuucgagca gcagaacgac acggaucagg aacggauccu gaauaccgag     1380

gccaagcaaa aacucuuuuc cuaccugaac cugcucgacc aucugacaga uaaugagauc     1440

cuuaacauuc ugggguauag ccccgacaag uggaaaauca auuuccgcga aauuaucgga     1500

aacagaacca auaacucuau gaugaaggcu uucuucaaua ugaaagagau gacagauggc     1560

gaacagauua acugggagaa gaaucucacc aaaaucacga acgggcaguc ccugaucagc     1620

ccggaacaga uuguggaccu gauccggcaa uuuuucgaga gagagggaau uacaaccucu     1680

cuucucuacu uugauccuac cacagacaau uuccgcgaac agucccacua uagccugugg     1740

caccuucucu acuccuacga ggaugacaac ucucccaccg gcacaacgag ccugugcaag     1800

acccuugcga agcgguaugg gaucccucau gaacuguguc cccuccuggc cucccugaca     1860

uuugagcccc aguacggauc ucuuagcacc aaagcuauga gaaagauucu cccuuucaug     1920

caagaaaacg gcuaugacaa agccugcgag cuggccggcu accgccacuc cgcuuggaca     1980

accaaggagg aaaauacgga gagaacccug aaaaacagcc ucccgcugcu ucggaagggg     2040

ucucugcgca aucccguggu cgaaaagauc cucaaccaga ugacacaugu gaucaauacc     2100

cacauuuccg aguauggaaa cccugaugaa aucagaauug agauggcccg ggacaugcag     2160

gcgaauagca aacagcgcaa ggcuaugaca accgccaucg ccgaggcuac gaaaagaaac     2220

acacaauuua uugaagagau ccagaaggaa uuuggcuuca aucccucuaa aaacgaucug     2280

aucaaguaca agcuguggaa agagcuugcc ccucucgggu ccaagacccu guauagcaau     2340

acauacauuc ccaaagagga acuguuuacc gcuaaguucg agaucgaaca cauuaucccg     2400

cgggcccucu uuuacgacga uucuuucagc aacaaaaccc uggagcuuaa uuccguuaac     2460

aagcugaagg gaaauaaaac agcgcucgac uauauggaua cgcugagcca ggaacaggcc     2520

aacgacuaca uuauccgcgu ggaggagcug aagaaaauug gcgcuaucac ccaagccaag     2580

uauaaaaagc ugcucaucag agaaaaagau cugggggacg auuuuacaaa ccgggaccug     2640

accaauacac gcuacauuau cagacaugcu gccgauaugc uccugcaggu caccccuucu     2700

auugugcaca cgaacggcuc caucacagcg auucuucgga gagacuggga gcugcucgac     2760

auccugaagg aacugaaucu ucccaccuau gagagcaucg aucgcaagau uuccgacccu     2820

aaagaaaagc ugaccgaguc uguugagcgg aaagauggac aggccaucaa gaugauuaaa     2880

gacuggagca agagagauga ccaucgccac caugcuaugg augcccucau cacagcucug     2940

accacacgga agcacgugca guaccucaac ggcaauagag gggccgaaga gacggacaaa     3000

guccaaaagc ugcagcagcu uaaagcccug cgcaagcaac ucauuggauc cgauggccac     3060

uucauucccc cuaugcauga cauccgggug caggcuaaag aagcgcuguc uagagucauc     3120

aucagcacca aggccaaggg gaaagugaca acccccaaca caaauauuau cuguucccgc     3180

gauaagacca cgggacggaa aauuuauguu aagcagacca cacugacccc gagaggcgcc     3240

cuucacaaag agaugcagua cgggugcaac cauuacuauc uggaaaagca aaucccuauu     3300

ggcucuaagc ucacagagga ggaaauccgg cuggugagcu cccccaaaau ccgcagcaug     3360

cucucugaga gacugcaggc uuacaauaac gaccccaagu ccgccuuuac cggacggaau     3420

agcauggaua aaaagccucu ugaagacaaa aacaagaagc uggucuauac agacaaaaag     3480

gugaaacaga ccauucucgc uaaucgcuuc guuaagagag aucgggugaa cucuaaacug     3540

gaccuggaua aggucaucga cacgggcauu aaaagaaucc uugagcgccg gcugaagcag     3600

uacgggggaa aguaugagac agccuuuucc gaucucgaaa auaacccgau uuggcugaac     3660

gaggaaaaag gcaucgcgau caagagcgug cggauucgcg ggaaaaauaa caccaucacc     3720

auucggcaaa caaauaccug uagaaucaag uacguggcua acgacaauaa ccaccaccuc     3780

gccguuuaug cccugcccga uggaucuauu gaggacgaua ucgucuccuu cuuugacgcu     3840

guggaacgca gccggaaagg ccuuccuauc auugauaagc auaagaauca gucccugggg     3900

uggaaauucc uguuuagcgu gaagcagaac gaguacuucc uccugcccca gcaaggcgag     3960

aaaaucgaca uugacacggg agaaaucucu augcagacaa ccuucagccc uaaggaucuu     4020

ucccccgagu acauuaugaa uccugacaac uaugccgaac uggcgcccca caucuuuaga     4080

guccagucua uuagcaaaac accgcagggc aaucgcgaug ugagaaucua ccucuucaag     4140

caacaguaua ucuccggguc ucagaaggac agccugcauc ucaaaggaau uacccaccgg     4200

caaaucuccu cugcuaagga ucuggccggc cuuguuaaaa uucgcgugaa ccaucugggg     4260

agaaucgucg ccgugggaga gaaguga                                         4287


<210>  4502
<211>  4287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4502
augaagaaaa uccugggccu cgaccugggg accgccagca uuggauccgc uuacgugcuu       60

gaggccgaaa agucugcgga ucagacaaaa aucgucuauc ugggcacgag aauuauccuc      120

agcuccgcug acgccgagaa cuucucuaag gggcugagcu gcucccccac cacagaucgg      180

accuuuaaac gcucuaugag acggcgccuu caaagauaca agcagcggcg cagcucccug      240

auucagcagu ucaagaaaau cggaaucauu acaaauaccg aacaaaucau uagagagccu      300

aucagcgaaa cacacgagcu ccugcggauu cgcucuaagg ccguuuccga gagagugagc      360

cuggaagagu uugcucggau ccuccugacc auuaacaaac gcagaggcua uaagucuaau      420

cggaaaacgu ccaagagcac cgaagaggac gggaaacuug uggaugccau ggcugucaca      480

aaggagcuga ucaagcucaa aaagaccccg ggacaguaca uguucgaacg cuuuaaaaga      540

ggcaaguaug ccgagaucga cuucuacccc uuugaucuga uuaacgaacu uaaacagauc      600

ugggaggugc agggccaaaa guauccugac guucugacag aagaucuccu gucuucccug      660

cucaagcaga aaaccgggaa ggcgaaagag cugauuuaca gcaaguccga ccuuaauccc      720

gccgaggugc cuaucgauua ccagaaagga aagaagcugc ggaaaacgau ucucaacaag      780

uauuucgcuu acgaaucuag aagccuggcc gucacaaaua ucauccccgu ggagcaggcu      840

auuaccgcca ucguugcgau uuguaaacaa aucaacaauu ccagcggcua ucuuggggcc      900

auuggagacc gcucucgggu gcuguacgcu aacaaucaga caaucggcca guaucucuac      960

gaaagaauca aggccaaccc ucaugaucgc auuagagggg aggucuuuua uagacaagac     1020

uaccuggacg aguucgaucg caucugggaa ugccagcaga auuacuaucc ggcuauucug     1080

accaacgacc ggaaacagua caucagaaau cgcauuaucu uuuaucaacg gccccucaag     1140

uccaagaaaa gcgagcuggc cuucugugaa uuugagucua gagaagugga gcacaacgga     1200

aagaaauaca ccgaaggccu ucauguggcc ccuaagagcu cccccgucuu ccagcacuuc     1260

cgccuguggc agcggcucgg ccagauugag guuucuccuc ugagaaaaug ggauaagcaa     1320

agcaacgagc uuuuugaaca gcagaauaac gcugaccggg aggggauccu gaauacagaa     1380

gcgaagcaga aacucuucuc cuaucugaac cugcucgauc aucugacgga caaugagauc     1440

cuuaacauuc ugggauacag ccccgauaag uggaaaauca auuuucgcga gauuaucggc     1500

aacagaacca auaaccucau gaugaaggcc uucuuuucua ugaaagaaau gacagacggg     1560

gagcaaauug auugggaaaa gaaucugacc aaaaucacaa acggacaguc ccugaucagc     1620

ccggagcaga uuguggaccu uauccggcag uucuuugaaa gagagggcau uaccacgucu     1680

cuccuguauu ucgauccugc uacagacaau uuucgcgagc aaucccacua cagccugugg     1740

caccuccugu acuccuauga agacgauaac ucucccaccg ggaccacaag ccuuugcaag     1800

acccuggcca agcgguacgg aaucccucau gagcucuguc cccugcuggc cucccuuaca     1860

uucgaaagac aguauggcuc ucucagcacg aaagcuaugc gcaagcugcu gcccuuuaug     1920

caggagaaaa gauacgacga agccugcgag cucgcgggcu aucggcacuc cgcuuggauu     1980

accaaggagg aaaaugccaa acgcacacug aagaacaaac uuccucugcu cagaaagggg     2040

agccugcgga acccgguggu cgagaagauc cugaaucaaa ugacccaugu gaucaacaca     2100

cacauugccg aauacggaaa ucccgaugag auccgcauug aaauggcuag agacaugcag     2160

gccaacucua aacagcggaa ggcuaugacc gccgcgauca cggaggccac caaacgcaau     2220

acacaguuca uugaggaaau ccaaaaggag uuuggcuuca acccuuccaa gaaugaucuu     2280

aucaaauaua agcucuggaa agaacuggcu ccccugggga acaagacccu cuacagcaau     2340

auuuacaucc cuaaagagga gcuguucaca gccaaguuug aaauugagca caucauuccc     2400

agagcucuuu ucuaugacga uucuuuuucc aacaagaccc uggaacucaa uagcguuaac     2460

aaacugaagg gaaauaaaac ggcccuggac uaccuugaua cacucggcca ggagaaggcg     2520

aacgacuaua uccugcgggu ggaggaacug aaaaagauug gggccaucac ccaggcuaaa     2580

uacaagaagc uccugauccg cgagaaagau cuuggcgacg auuucacaaa uagagaccug     2640

accaacaccc gguauauuau cagacaugcc gcugacaugc uccugcaggu cacaccgucu     2700

auugugcaca cgaacggaag caucaccgcc auucugcgcc gggauugggg ccuucuggac     2760

auccucaagg aacugaaucu cccuacauac gaguccaucg auagaaaaau uagcgacccc     2820

gaagagaagc ugacccaauc uguugaucgc aaagacgggc aggccaucaa gaugauuaag     2880

gauuggucca aacgggacga ucauagacac caugcuaugg acgcgcuuau cacagcccug     2940

accacgcgca agcacgugca guaucucaac ggaaauagcg gcgcugaaga gacagauaaa     3000

gcccagaagc uggagcaacu gaaagcccuu cggaagcagc ugauuggguc cgacggacac     3060

uuuaucccuc ccaugcauga uaucagaguc caggcuaagg aagcccucuc ucgcgugauu     3120

aucagcacca aagucaaggg caaagugacc acaccuaaca ccaauauuau cuguucccgg     3180

gacaagacaa cggggagaaa aauuuacguu cggcaaacca cacugacacc ccgcggcgcg     3240

cuccaccugg acaccaagua cggaaucaac agauauuacc aggagcggga agugucuauu     3300

ggcagcaaac uuacccagga ggaaauccgc cuggucuccu cuccgcauau ccagagcauu     3360

cuccuggaga gacugcgggc uuucgauaau aacccuaagu ccgccuuuac ggggagaaau     3420

agcguggaca agaaaccccu ugaggauaag aacaaaaagc ugguuuauac cgacaagaaa     3480

gugaagcaaa caauccucgc caaucgcuuc gucaaacggg aucaggugaa cucuaagcug     3540

aaucucgaca aagugauuga uaccggaauc aagcggauuc uggaaggccg ccuucagcag     3600

uacgggggaa aauaugagac agcuuuuucc gaccuggaaa acaaucccau cuggcugaac     3660

gaggagaagg gcaucgccau uaagagcguu cggaucagag ggaaaaacaa ugcgauuccu     3720

auccaccaaa ccgauacaug cccgauugac uacgucgcua acgauaauaa ccaccaucuc     3780

gccguguaug ugcugcccga cggcaccauc gaagacgaua ucgucacguu cuuugacgcc     3840

guggagcgcu cucggcaggg acuuccuauu guugauaaga auaaaaacca gucccugggc     3900

uggaaguucc ucuuuagcau caaacagaau gaauacuucc ugcuccccca acaggcugag     3960

aagauugaca ucgauacagg ggaaauuucu acccagacca cauucucccc uaaggaccug     4020

agccccgagu acaucaugaa cccugauaau uaugccgagc uugcuuccca cauuuuuaga     4080

gugcagagca ucucuaaaac gccccaagga aaccgcgacg ucagaaucua ccuguucaag     4140

cagcaguaua uuagcggcuc ccaaaaagau ucucugcauc ucaaggggau caccuaccgg     4200

cagauuagcu ccgccaaaga ccuggcggaa cuugugaaga uccgcguuaa ucaccuggga     4260

agaauugugu cugucggcga gaaguga                                         4287


<210>  4503
<211>  4287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4503
augaccaaga aaauccuggg ccucgaccug gggacaacga gcauuggaug ggccuacgug       60

aucgaguccg auaaccccga cgaaucugag auuaagcaga ucggcgucag aguuaauccu      120

cuuaccacag augaacaaac caacuucgag aaagggaagc cguuuagcau uaauaaagaa      180

cggacacuga agcgcggagc uagacggaac cucgaccgcu auaaggauag acggaauaac      240

cugaucgagg ugcuuaaaaa ggccaauauc cugucccagg acucuauucu caccgaggaa      300

ggccagcaca caacccauca gacguggcaa cugcgcgcga aagcugccgu ggauaagauc      360

gagaaagaag aacuggccag aguccuccug gcuauuaaca agaagcgggg guacaaaagc      420

ucccgcaagg ccaaaaauga ggacgaagga caggcuaucg auggcauggc cguggcgaag      480

agacuuuaug acgagcagau uaccugcggc cagcugguuu accaacuccu gcgggaaggg      540

aaaaagucua uccccgauuu cuaucgcagc gaccuucagg ccgaguuuga uaaagugugg      600

aacuuccaga agcaguuuua cccuaagauu cugacagacg aguucuauaa agagcucaag      660

ggaaaaggcc aaagagcuac cuccgcccug uuuuacaaga cauacaauuu caacaccgcu      720

gagaauaaag ggagccggga agagaagaag auccaggccu auaaauggcg cucugaagcg      780

aucucccagc aacuggagaa ggaagagguc gccuuugugc ucacggagau uaacaauaac      840

cugaacaaua gcucuggaua ccuuggcgcu aucuccgaca gaagcaaaga acuguauuuc      900

aacaagcaga caguugggca guaccucuau cagcaacugc aggagaaucg gcacgccaga      960

cuuaaaaacc agguguuuua ccgccaggau uaucuggacg aguucgaggc uauuuggaag     1020

gaacaagcca agcaucacac cgagcucaca gaggaacuga aagccgagau ccgggauauu     1080

aucaucuuuu accagagaaa gcugaaaucu cagaagggac ucauuuccuu cugugaguuc     1140

gaaagccgcg agauccagau ugacggcaaa aagaagacca ucggccugcg ggucgcuccc     1200

aaauccucuc cucuuuuuca agaguucaag auuuggcaga aucugaacaa ugugcucgug     1260

agaaagaagg ggagccgcaa acggaacguc aagcaggaaa uccagggauc ccuguuugaa     1320

gaggaaaaag agaucuucgu uuuugaucuu gagaccaagg aaaagcuguu cgaggaacuc     1380

aaucugaaag gcaaccugag cgcgaauaag gugauugagc uccuggggca uaaacccaca     1440

gaaugggaga ucaacuacuc ugcccuugag ggaaauagaa cgaaccaagc ucuguauaau     1500

gccuaccuca agauucugga caucgaaggc uaugaugagg accugcuuaa acucuccgau     1560

aaggacgaua uuaacguggc cgaccugaag accccugcua gcgaaaucaa aaacaugguc     1620

cgcgacauuu uucaggugcu ggggaucucu acagauaucc ucgaguucga cgccgaacug     1680

gauggaaagg acuuugagca gcaagcgagc uaccagcuuu ggcaucugcu cuauuccuac     1740

gagggcgaug acucucagag cggcaaugaa aaacuguaug agcugcuuga aaagaaauuc     1800

ggguuuaaga aggagcacuc ccagguucuc gcuaacguga cccugagcga ugacuacgga     1860

aaucugucuu ccaaagccau gcggaagauu uacccguuca ucaaagagaa caauuauagc     1920

acagccugcg aacucgcugg cuacagacac ucuaagcacu cccugaccaa agaggaaauu     1980

gagaaccgcc cccuugauga caagcuggaa cuccugaaga aaaauagccu gcggcaaccu     2040

gucguggaga agauccuuaa ccagaugguu aaugugguca acucccuccu ggagaaaaau     2100

agaaaggaaa accccgauuu ucgguucgac gagauucgca ucgaacuggc cagagagcuc     2160

aaaaagaaug cuaaagaacg ggccgagaug acgacaaaca ucaauucugc gaagaccgag     2220

aacgaaaaga uuuucaaacu gcuucaggcc gaguuugggc ugaagaaccc uaccagaaau     2280

gauaucauuc gcuaucggcu cuacgaagaa cugaaaaaca auggauauaa ggaccuguac     2340

acaaacaaau auauccccag agaaauucuu uucagcaaac agguggacau cgagcauauc     2400

auuccgcaau cccgccucuu ugaugacucu uucagcaaua agaccaucgu cuacagagag     2460

acaaaccugg auaaaggcaa ucagacggcu uuugacuaca uugauuccaa guaugggaac     2520

gaaaaagugg aggaauacau ccugcggauu gaggcccucc acgacgcuug gaagaaaaau     2580

aaggaggaag gcaucagcaa ggccaaauau cagaagcugc uuaaaaagga gaccgaaauc     2640

ggagauggcu ucauugagcg cgaccugaga gaaucucagu acaucgcgaa aaaggccaag     2700

gagaugcucu uuaaaauuac acgguccguu gugagcaccu cuggguccau cacagauaag     2760

cugcgcgagg acuggggacu gauuaacauc augaaagaac uuaaucugcc uaaguauaga     2820

agccucggcc ugaccgagau gcaagaacgg aaauucgggc agaaggucga ggugauuaac     2880

gauuggacga agcgcaauga ccacagacau cacgcuaugg augcccucac cguugcuuuu     2940

acaaaacaua accacaucca guaccugaau uaucuuaacg cccggaagaa cgaaaaucau     3000

aaacugcaca acaauaucau ugccaucgag aaccucgaga ccgaaacaau gauugacaau     3060

aacggaucua agcgcagagu guucaaagcu cccaugccua auuuucggca agucgcgaag     3120

gagcaccugg aaaacgugcu gaucagccau aaagccaaga auaagguggu uaccaaaaac     3180

aagaauaaaa uugcuggcaa ggucgccgcc caggagacgc uuacacccag agggcagcug     3240

cacaaagaaa ccguguacgg caaguaccag uauuacguga acaaggagga gaaaaucgga     3300

acaaaguucg acgaagagac caucaauaaa gucuccaacc cccaauaucg caagcuccug     3360

cucgaacggc ugagcgcuaa uggcaacgau ccuaaaaagg ccuuuucugg gaagaaugcg     3420

cuuuccaaaa acccgauuua ccugaacgag accaagacag agacggugcc cgaaguugug     3480

aaacucaccu ggcuggagga agacuauagc aucagaaagg auauuacacc ugacaauuuc     3540

aaagaugaga aguccaucga caagauucug gaugaaggag ucaaacgcau ccuuaagaaa     3600

cggcugagaa agaacgacaa ugaucccaaa aaggcuuucu cugaccucga uaaaaacccu     3660

aucuggcuga augaggagaa gggcauuagc aucaagcgcg ugaccauuuc cgggguuaac     3720

aaugccgaac cccuccauua caaaaaggac cacuuuggaa acgacauccu ggaugagaaa     3780

ggcgacaaga uuccggugga uuucgucucu acagggaaua accaccaugu ggccguuuau     3840

cgggacgaaa aaggcaaucu ucaggagaac aucgugagcu uuuucgaagc ugucauuaga     3900

gugaaucagg gacugccuau caucgauaag gccuacaacc agcaccuggg cuggcaauuu     3960

cucuucacca ugaagcagaa ugagauguuu guguucccca acgagaaauc cggguuuaac     4020

ccucaggaaa uugaccugcu ugaucccucu aauaagaaaa agaucagccc uaaccuguuc     4080

cgcguccaga aaauguccaa gguuauuuac ggaaauagcc ccgugcggga guauguguuu     4140

agacaucacc ucgaaacgaa cgucgacgag aagaaagaac ugaaggauau cacauucaaa     4200

aauauuaagu cucucggcua cuucgaggac aucgugaaag uucggaucaa ccaucugggg     4260

gauauugugu ccgucggaga guauuga                                         4287


<210>  4504
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4504
augaagcaca uccugggccu cgaccugggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augauaacga acaauccgag aucguugacg ugggcgugag agucaauccc      120

cuuacaacgg augaaaagac caacuucgag aaagggaagc cuauuucuac aaaugccgac      180

cggacccuga aacgcagcgc gagacggaac cuccagcgcu acaagcugag acggaaggaa      240

cuuaucgagc ugcucaaaga gcaucagcug auuuccacag auaccauccu gacagaaaau      300

ggaaagggca ccacguuuca gacccucgag cugcgcgcua aaucugccaa ggaaaagauc      360

agccuugagg agcuggccag aguguuccuc acaauuaaca agaagcgggg guauaaaucc      420

ucucgcaagg cuaaaaauga agacgaggga caacugaucg auggcaugga aauugccaag      480

agacuuuacg aggaaaaccu gaccacaggc cagcucagcc ugcagcugcu caaagagggg      540

aagaaauccc ugccggacua uuaccggagc gaucuucagu uugagcugga caagguuugg      600

aauuuccaaa agcaguuuca cucugauauc cucaccgacg aauuuuaugc ucagcugaaa      660

ggaaagggcc agcgcgccac guccgcgcuu uuucuggcca aauaccaaau uuauacagcu      720

gagaacaagg ggagcagaga agauaaaaag cuccaggccu accaguggcg gguggacgcu      780

cugucuaagg agcugacccg cgaacaacuc gccuucguca ucacagagau uaauaacaau      840

cugaacaacu ccagcggaua ccuuggcgcg aucucugaca gauccaaaga gcuguauuuu      900

aauaacgaaa ccguggggga guaccucugg aagcagaucg ccgcuaaucc ccauaccagc      960

cugaaaaacc agguuuucua ucggcaggau uaccuugacg aauuugagca aauuuggaag     1020

acacaguccg ccuucuaucc ugaaauccug acggaugagc ucaaaaagga gcugagagac     1080

guggucauuu ucuaccagcg caagcugaaa ucucaaaaga gccucguguc cuucugcgag     1140

uucgagaaua aagaaaucga gauuaagaaa gaggauggaa ccagcacaaa gaaggugauc     1200

ggccugaaag ucgcucccaa gucuuccccu cuuuuucagg aguucaaaau cuggcagaac     1260

cugaauaacg uucucauucg gaagaaaggc agcaagacca aaaagguggc caagaaucag     1320

caggccgugc uguuugaaga agagaaaaca aucucugaac uggaccuuga ggcuaagcaa     1380

gcccucuuca acgagcugaa ucugaaaggg aaccucaagg cggauuauug ucuggaaauu     1440

cuuggauaca gccccaaaga gugggaaaug aauuacuccg uccuggaggg caacagaacc     1500

aauaaggcuc ucuaugaagc cuaccugaag aucacggacc uggaggggua ugaugccaaa     1560

aaccaccuua aggugaaacu caacaaggac gagguugaac uggaugaccu gaaagugccu     1620

gcuucugaga uuaaggacau gguccaggcc aucuuuaagg aagcgggaau caauacagau     1680

auucuccauu ucaacgcuga gcuggacggc aaagauuuug aaaaucaggc cagcuaccag     1740

cuuuggcauc ugcucuauuc cuacgagggg gacgauagca agucuggaaa cgagcugcug     1800

uauaaacuuc ucgaaaccaa guucggcuuu aaaaaggagu acgcccaaau ccuggcuaau     1860

gugucccuga gcgacgauua cggcucucuc uccaccaagg ccaugcgcaa aauucacccg     1920

uauaucaagg aaaacaaauu cagcacagcu ugcgagcugg ccggguaccg gcacuccaag     1980

cacucucuua ccaaagagga aaaugacaac agaguucuga aggauagccu cgaggugcug     2040

aaaaagaacu cccugcgcca gcccgucgug gaaaagauuc uuaaucagau gaucaaucuc     2100

gucaacgcgc ugcugaaaaa guaucggaca gagaauccua acuuuaaauu cgacgaaauu     2160

agaaucgagc ucgcccgcga gcugaagaaa aaugcugagg aacgggccaa caugacggcu     2220

aacaucaaua aggccaagac cgaacaugag aaaauuauca agauucuuaa aaacgaauuu     2280

ggacugccca auccuacaag aaacgauauc auucgcuacc ggcucuauga ggagcugaag     2340

gcgaauggcu acaaagaccu guauaccaac aucaagaucc cccaagaaaa acuuuucaca     2400

aaagagauug auaucgaaca cauuaucccg aagucuagag uguucgacga cagcuuuucc     2460

aauaaaaccc ugagcuuccg gaagacgaac cucgauaaag gggagcgcac cgccuuugac     2520

uacauggaau cuaaguucgg agcugauaag cuggaggagu acguugccag aguggaagac     2580

cucuauaaaa auaacauuau cuccaaggcu aaauaccaga agcugcuuaa aaaggagagc     2640

gaaaucggcg auggguuugu cgagcgggac cugagagaga cacaguauau ugccaagaaa     2700

gccaaggaaa uccuccugca gauuaccccu aaugugcugu cuacauccgg cggaaucacc     2760

gaucgccuuc gggaggacug ggaucugauu aacguuauga aagaacucaa ucugcccaag     2820

uacagagcuc ucggccugac gcaaauggag gaccgcaaau augggcaaca gguggaaguc     2880

aucacagauu ggaccaagag aaacgaccac cggcaucacg cgauggaugc ccuuacagug     2940

gcuuucacca aacauaauca cauccaguac cugaacuauc ucaacgcccg caagaaugag     3000

aagcauaaag agcacagcaa cauuauggga auccagcagc uggaaaccau uaaggugaca     3060

gacaaaaaug gcaacgagaa gagaguuuuu aaagccccua ugcccaauuu ccggcagguc     3120

acgaaggcuu uucuggaaaa cauccuuauu ucucacaagg ccaaaaauaa gguggugacc     3180

aaaaacaaga auaaagucgc ggggagcaac aaggugcagg agguucugac accccgcgga     3240

caacuccaua aggaaaccgu guacggcaaa uaccaguauu acguccagaa ggaggagaaa     3300

aucggggcua aguucgaccu ggaaacaauu aaaaaggugg ccaauccucu cuaucaaucc     3360

cugcuuaaga aaagacugga ggaaaacggc ggagauccga agaaagccuu uaccggcaag     3420

aauagccugg cuaaaaaccc caucuaccuc gacgauacgc agacagagca gcugccugaa     3480

aagguuaaac uuguguggcu ggaaaauucu uucuccaucc ggaaggacau uacccccgag     3540

aacuuuaagg augaaaaacu caucgacaag auucuggaug agggggucaa acgcauccuc     3600

aaggaaagac ugcagagcuu cggaaaugac gccaaaaagg cguucuccga ucuugacaag     3660

aacccuauuu ggcugaacaa agagaagggc aucaccauca aacgggugac aauuucuggg     3720

guugcuaaug ccgagccccu gcacaccaag aaagaucauc ucggaaagga aauccuggac     3780

aaggagggca aaccgauucc uguggacuuu aucagcacag gguccaacca ccacgucgcc     3840

guguaucgcg augaaaaggg caaucuucaa gagaaagugg ucucuguuuu cgaagcugug     3900

gugagagcca accagggacu gcccauuauc gacaagacgu acaaucagca ucucggcugg     3960

gaguuucugu ucaccaugaa acagaacgag auguuugucu ucccuaauga aagcacaggg     4020

uuuaacccca augauaucga ccuccuggau ccuaagaaca agcggauuau cucccccaau     4080

cuuuucagag ugcaaaaauu uucuaagcug caguauggaa acagcuucgu ucgcgaguuu     4140

guguuccggc accaucugga aaccacaguc gaggacagaa aagaacucca gggcauuacc     4200

uacaagcaga ugaaaaauuc cacguucuuu aacgauauca uuaaggugcg caucaaucac     4260

cugggggaca uuguuagcau cggagaguac uga                                  4293


<210>  4505
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4505
augaagcaca uccugggccu cgaccugggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augauaacga acaauccgag aucguugacg ugggcgugag agucaauccc      120

cuuacaacgg augaaaagac caacuucgag aaagggaagc cuauuucuac aaaugccgac      180

cggacccuga aacgcagcgc gagacggaac cuccagcgcu acaagcugag acggaaggaa      240

cuuaucgagc ugcucaaaga gcaucagcug auuuccacag auaccauccu gacagaaaau      300

ggaaagggca ccacguuuca gacccucgag cugcgcgcua aaucugccaa ggaaaagauc      360

agccuugagg agcuggccag aguguuccuc acaauuaaca agaagcgggg guauaaaucc      420

ucucgcaagg cuaaaaauga agacgaggga caacugaucg auggcaugga aauugccaag      480

agacuuuacg aggaaaaccu gaccacaggc cagcucagcc ugcagcugcu caaagagggg      540

aagaaauccc ugccggacua uuaccggagc gaucuucagu uugagcugga caagguuugg      600

aauuuccaaa agcaguuuca cucucauauc cucaccgaug aauuuuaugc ucagcugaaa      660

ggaaagggcc agcgcgccac guccgcgcuu uuucuggcca aauaccaaau uuauacagcu      720

gagaacaagg ggagcagaga agacaaaaag cuccaggccu accaguggcg gguggaugcu      780

cugucuaagg agcugacccg cgaacaacuc gccuucguca ucacagagau uaauaacaau      840

cugaacaacu ccagcggaua ccuuggcgcg aucucugaca gauccaaaga gcuguauuuu      900

aauaacgaaa ccguggggga guaccucugg aagcagaucg ccgcuaaucc ccacaccagc      960

cugaaaaacc agguuuucua ucggcaggac uaccuugaug aauuugagca aauuuggaag     1020

acacaguccg ccuucuaucc ugaaauccug acggacgagc ucaaaaagga gcugagagau     1080

guggucauuu ucuaccagcg caagcugaaa ucucaaaaga gccucguguc cuucugcgaa     1140

uuugagaaua aagaaaucga gauuaagaaa gaggacggaa ccagcacaaa gaaggugauc     1200

ggccugaaag ucgcucccaa gucuuccccu cuuuuucagg aguucaaaau cuggcagaac     1260

cugaauaacg uucucauucg gaagaaaggc agcaagacca aaaaggugga uaagaaucag     1320

caggccgugc uguuugaaga agagaaaaca aucuucgaac uggaccuuga ggccaagcaa     1380

gcucucuuua acgagcugaa ucugaaaggg aaccucaagg cguauuacug ucuggaaauu     1440

cuuggauacu cucccaaaga gugggaaaug aauuauagcg uccuggaggg caacagaacc     1500

aauaaggccc ucuacgaggc uuaucugaag aucacggauc uggaagggua cgacgccaaa     1560

aaccaucuua aggugaaacu caacaaggau gagguugaac uggacgaucu gaaagugccu     1620

gccuccgaga uuaaggacau gguccaggcu aucuucaagg aagccggaau caauacagac     1680

auucuccacu uuaacgcgga gcuggauggc aaagacuucg aaaaucaggc uucuuaucag     1740

cuuuggcauc ugcucuacag cuaugagggg gaugacucca agagcggaaa cgagcugcug     1800

uacaaacuuc ucgaaaccaa guuuggcuuc aaaaaggagu acgcccaaau ccuggccaau     1860

gugucucugu ccgaugacua uggcagccuc ucuaccaagg cuaugcgcaa aauucacccg     1920

uacaucaagg aaaacaaauu uuccacagcc ugcgagcugg cuggguaucg gcacagcaag     1980

cauucccuua ccaaagagga aaaugauaac agaguucuga aggacucucu cgaggugcug     2040

aaaaagaaua gccugcgcca gcccgucgug gaaaagauuc uuaaccagau gaucaaucuc     2100

gucaacgccc ugcugaaaaa guaccggaca gagaauccua acuucaaauu ugaugaaauu     2160

agaaucgagc ucgcgcgcga gcugaagaaa aaugccgagg aacgggcuaa caugacggug     2220

aacaucaaua aggccaagac cgaacacgag aaaauuauca agauucuuaa aaacgaauuu     2280

ggacugccca auccuacaag aaacgacauc auucgcuauc ggcucuacga ggagcugaag     2340

gcuaauggcu auaaagaucu guacaccaac gaauacaucg agaagcaaga ccuuuucaca     2400

aagaauaucg auauugaaca caucauuccc aaauccagag uuuuugacga cucuuucagc     2460

aacaagaccc uguccuuucg gaaaacgaau cucgauaagg gggagcgcac cgccuucgac     2520

uauaucgaaa gcaaauuugg agcggauaag cuggaggagu acguggccag agucgaagac     2580

cucuauaaga acaauauuau cucuaaagcu aaguaccaga aacugcuuaa gaaagagucc     2640

gaaaucggcg auggguucgu ggagcgggac cugagagaga cacaguauau ugccaagaag     2700

gcuaaagaaa uccuccugca gauuaccccg aacguucuga gcacaucugg cggaaucacc     2760

gaucgccuuc gggaggacug ggaucugauu aaugugauga aggaacucaa ccugccuaaa     2820

uacagagccc ucggccugac gcaaauggag gaccgcaagu augggcagca ggucgaagug     2880

aucaucgauu ggacaaaaag aaaugaccau cggcaccaug ccauggaugc ucuuaccgug     2940

gcguuuacaa agcacaacca uauucaguac cugaacuacc ucaaugcccg caaaaacgag     3000

aagcacagag agcacuccaa uaucaugggc auucaacagc uggaaaccau caagguuacc     3060

gacaaaaacg gaaaugagaa gcgggucuuc aaagcuccca ugccuaacuu ucgccaggug     3120

acaaaggccu uccuggaaaa ugugcuuauu agccauaaag ccaagaacaa ggucgugacg     3180

aaaaauaaga acaaaguugc ugggucuaau aaggugcagg agguccugac ccccagagga     3240

caacuccaca aagaaacagu guauggcaag uaccaguauu acguucagaa ggaggagaaa     3300

aucgggggca aguuugaccu ggaaaccauu aaaaaggugg ccaacccccu cuaucaaagc     3360

cugcuuaaaa agcggcugga ggaaaaugga ggcgauccua agaaagcguu cacagggaag     3420

aacucccugg cuaaaaaucc gaucuaccuc gacgaugccc agaccgagca gcugcccgaa     3480

aaggucaaac uuguguggcu ggagaacagc uuuucuaucc gcaaggacau uacgccugag     3540

aauuucaaag augaaaagcu caucgacaag auucuggaug agggaguuaa aagaauccuc     3600

aaggaacggc ugcaguccuu cggcaacgac gccaaaaagg cuuuuagcga ucuugacaaa     3660

aaccccauuu ggcugaauaa ggagaagggg aucgccauca aacgcguggc gauuuccgga     3720

gucgcuaacg ccgagccucu gcauacaaag aaagaucacc ucggcaagga aauccuggac     3780

aaagagggga agucuauucc cguggacuuc aucagcaccg gcaauaacca ccauguggcc     3840

gucuauagag augaaaaggg aaaucuucaa gagaaaguug uguccguguu ugaagcuguc     3900

gugcgggcca accagggccu gccgauuauc gacaagaccu acaaucagca ccucgggugg     3960

gaguuccugu uuacaaugaa acagaacgag auguucguuu ucucuaauga aagcaccgga     4020

uucaacccua augauaucga ccuccuggau cccaagaaca aaaagauuau cuccccuaau     4080

cuuuuuagag ugcaaaaguu cucuaaacug caguacggca acagcuuugu ccgcgaguuc     4140

guguuccggc aucaccugga aacaacgguu gaggacagaa aggaacucca ggggauuacc     4200

uauaaacaga ugaaaaauuc cgcuuucuuc aacgauauca uuaaagugcg caucaaccau     4260

cugggaggca uugucagcgu gggggaguac uga                                  4293


<210>  4506
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4506
augaaggaca uccugggccu cgaucugggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augacaacga aacauccgag aucguugauc uuggcgugag agugaauccc      120

cugacgaccg acgaaaagac aaacuucgag aaagggaagc cuauuucuac caaugccgau      180

cggacacuca aacgcagcgc gagacggaac cugcaacgcu acaaacuuag acggaaggaa      240

cugaucgagc uccugaaaga gcaccagcug auuuccaccg acacaauccu caccgaaaau      300

ggaaagggca cgucuuuuca gacccuggag cuucgcgcua aaagcgccaa ggagaaaauc      360

ucccuggagg agcucgccag agucuuccug acaauuaaca agaagcgggg guauaaaucu      420

agccgcaagg cuaaaaauga agaugaggga cagcuuaucg acggcaugga aauugccaag      480

agacuguacg aggaaaaccu caccacaggc caacuguccc ugcagcuccu gaaagagggg      540

aagaaaagcc uuccggauua uuaccggucu gaccugcagu uugagcucga uaaggugugg      600

aauuuccaga agcaauuuca uucccacauc cugaccgacg aguucuaugc ucagcuuaaa      660

ggaaagggcc agcgcgccac gagcgcgcug uuucucgcca aauaccagau uuauacagcu      720

gaaaacaagg ggucuagaga agauaaaaag cugcaagccu accaguggcg gguugacgcu      780

cuguccaagg agcucacccg cgaacagcug gccuucguga ucacagagau uaauaacaau      840

cuuaacaaca gcucuggaua ccugggcgcg aucuccgaca gaagcaaaga gcucuauuuu      900

aauaacgaaa ccgucgggga guaccugugg aagcaaaucg ccgcuaaucc ccauaccucc      960

cuuaaaaacc agguguucua ucggcaggau uaccuggacg aauuugagca gauuuggaau     1020

acacaagccc aguucuaucc ucagauccuc acggaugaac ugaagaaaga gcugagagac     1080

guugugaucu uuuaccagcg caagcucaag ucucagaaaa gccuggucuc cuucugcgag     1140

uucgaaaaca aggagauuga aauuaaaaag gaggauggaa ccagcaccaa aaaggugauc     1200

ggccuuaagg uggcucccaa aucuuccccu cuguuucagg aguucaagau cuggcagaau     1260

cucaacaaug uccugauucg gaaaaagggc agcaaaacca agaaagccgc caagaaccag     1320

caagcuguuc uguuugaaga ggaaaagaca aucucugagc uugaccucga agcgaaacag     1380

gcccuguuca augagcugaa ccucaagggg aaucugaaag cugauuauug ucuugagauu     1440

cugggauaca gccccaagga augggagaug aacuacuccg ugcucgaagg caauagaacc     1500

aacaaagccc uguaugaggc cuaccugaag aucacggacc uugaagggua ugaugcuaag     1560

aaccaccuca aagugaagcu gaauaaagac gaggucgagc uggaugaccu caaggugccu     1620

gccucugaaa uuaaagacau gguucaggcg aucuuuaagg aggcuggaau caacacagau     1680

auucugcauu ucaaugccga acuugacggc aaggauuuug agaaccaggc cagcuaccaa     1740

cuguggcacc uccuguauuc cuacgaaggg gacgauagca aaucuggaaa ugagcugcuu     1800

uauaagcucc uggagaccaa auucggcuuu aagaaagaau acgcucagau ccuggccaac     1860

gugucccuca gcgacgauua cggcucucug uccaccaagg cuaugcgcaa gauucacccg     1920

uauaucaaag agaauaaguu cagcacagcc ugcgaacuug cgggguaccg gcauuccaaa     1980

cacucucuga ccaaggagga gaacgacaau agaguccuca aagauagccu ggaagugcug     2040

aagaaaaacu cccuucgcca gcccguugug gagaagauuc ucaaucaaau gaucaaccug     2100

gucaaugccc ugcucaagaa auaucggaca gaaaacccua auuuuaaguu cgacgagauu     2160

agaaucgaac uggcucgcga gcuuaaaaag aacgccgagg aacgggcuaa caugacggcc     2220

aauaucaaca aagcgaagac cgagcaugaa aagauuauca aaauucugaa gaaugaguuu     2280

ggacucccca acccuacaag aaaugauauc auucgcuacc ggcuguauga ggaacugaaa     2340

gccaacggcu acaaggaccu uuauaccaau aucaaaaucc cccaggagaa gcuguucaca     2400

aaggaaauug auaucgagca cauuaucccg aaaucuagag uguucgacga cagcuuuucc     2460

aacaagaccc ucagcuuccg gaaaacgaau cuggauaagg gggaacgcac cgcuuuugac     2520

uacauggagu cuaaauucgg agccgauaag cucgaagagu acgucgcuag aguggaggac     2580

cuguauaaga acaauauuau cuccaaagcc aaguaccaga aacuucugaa gaaagaaagc     2640

gagaucggcg auggguuugu ugaacgggac cucagagaga cacaguauau ugccaagaag     2700

gcuaaagaga uccugcugca aauuaccccu aacgugcuuu cuacauccgg cggaaucacc     2760

gaucgccugc gggaagacug ggaucucauu aaugucauga aggagcugaa ccuccccaaa     2820

uacagagcgc ugggccuuac ggaaauggag gaccgcaagu augggcaaca gguggaaguu     2880

aucaucgauu ggacaaaaag aaaugaccac cggcaucacg ccauggaugc ucugaccgug     2940

gccuucacaa agcauaacca cauucaguac cucaacuauc ugaaugcccg caaaaacgag     3000

aagcauaagg agcacagcaa uaucaugggc auucaacagc uggaaaccau caaagucacc     3060

gacaagaacg gaaaugagaa aagaguguuu aaggcuccua ugcccaacuu ccgccaggug     3120

acaaaagccu uucuugaaaa uauucugauc ucucacaagg cgaagaacaa aguugucacg     3180

aagaauaaaa acaagguggc ugggagcaau aaagugcagg agguccucac accccgggga     3240

caacugcaua aggaaacagu guacggcaag uaccaguauu acguucagaa agaggagaag     3300

auugggggca aauucgaccu cgaaaccauc aagaaagugg ccaacccucu guaucaaucc     3360

cuucugaaga agagacugga ggaaaaugga ggcgauccga aaaaggccuu uacagggaaa     3420

uacagccucg cuaagaaccc caucuaucug gacgaugccc agaccgagca gcuuccugaa     3480

aaagucaagc ugguguggcu cgaaaauucu uucuccauuc ggaaagacau cacgcccgag     3540

aacuuuaagg augaaaagcu gauugacaaa auccucgaug agggaguuaa gcgcauucug     3600

aaagaaagac uucagagcuu cggcaaugac gcgaagaagg cuuucuccga ucuggacaag     3660

aacccuaucu ggcugaacaa ggagaaaggg aucacaauua agcgggugac caucucugga     3720

gucgccaaug ccgagccccu ccacaccaaa aaggaucauc ugggcaaaga aauucuugac     3780

aaggagggga agccgauccc uguggacuuu auuagcacag gcuccaacca ccacguugcu     3840

guguaccgcg augaaaaagg aaaucugcaa gagaaggucg ugucuguguu cgaagccguc     3900

guuagagcua accagggccu ccccaucauc gacaaaaccu auaaucagca ucuggggugg     3960

gaguuucucu ucacaaugaa gcagaacgag auguuugugu ucccuaauga aagcacggga     4020

uuuaacccca augauauuga ccugcuugau ccuaaaaaca agcggaucau uucccccaau     4080

cuguucagag ugcaaaccau cucugucgug aaguacggca acgccauugu ucgcgacuuu     4140

aaauuccggc accaucugga gacaaccgug gaagauaaga aagagcucca ggggaagaca     4200

uaccagcaga ucaaaagccu guccccgcuu aaugacauua ucaaggucag aaucaaccac     4260

cugggagaua uugugagcgu uggcgaauau uga                                  4293


<210>  4507
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4507
augaagcaca uccugggccu cgaccugggg accaacagca uuggaugggc cuacgugaaa       60

gaggcugaaa augauacaga gacguccgaa aucgucgacc uuggcguuag agugaacccc      120

cugaccacag augagaagac caauuucgaa aaagggaagc cuauuucuac aaacgccgac      180

cggacccuca aacgcagcgc gagacggaau cugcagcgcu auaaacuuag acggaaggag      240

cugaucgagc uccugaaaga acaucaacug auuuccgaga acacaauccu caccgaaaau      300

ggaaagggca cgaccuuuca gacacuggag cuucgcgcua aaucugccaa ggagaaaauc      360

agccuggggg aacucgccag aguguuccug accauuaaca agaagcgggg auacaaaucc      420

ucucgcaagg cuaaaaauga ggaugaaggc cagcuuaucg acggcaugga gauugccaag      480

agacuguaug aagagaaccu cacaaccggg cagcugagcc ugcaacuccu gaaagaggga      540

aagaaauccc uuccggauua cuaucggagc gaccugcagu uugaacucga uaaggucugg      600

aauuuccaga agcaguuuca cucugacauc cugacggaug aguucuacgc ucaacuuaaa      660

ggcaaggggc agcgcgccac auccgcgcug uuucucgcca aauaucagau uuacaccgcu      720

gaaaacaagg gaagcagaga ggacaaaaag cugcaggccu accaauggcg gguggaugcu      780

cugucuaagg aacucacacg cgagcagcug gccuucguua ucaccgagau uaauaacaau      840

cuuaacaacu ccagcggcua ucugggggcg aucucugaca gauccaaaga acucuacuuu      900

aauaacgaga ccgugggaga auaucugugg aagcagaucg ccgcuaaucc ccauacaagc      960

cuuaaaaacc aagucuucua ccggcaggac uaucuggaug aguuugaaca gauuuggaau     1020

acgcaggccc aauucuaccc ucagauccuc accgacgagc ugaagaaaga gcugagagau     1080

gugguuauuu ucuaucagcg caagcucaag ucccagaaau cucuggugag cuucugcgaa     1140

uuugagaaca aggaaaucga gauuaaaaag gaggacggca cauccaccaa aaaggucauc     1200

ggccuuaagg uggcucccaa aagcucuccu cuguuucaag aguucaagau cuggcagaau     1260

cucaacaaug ugcugauucg gaaaaagggg uccaaaacaa agaaagccgc caagaaccag     1320

caggcugucc uguuugaaga agagaagacc aucagcgaac uugaucucga ggcgaaacaa     1380

gcccuguuca augagcugaa ccucaaggga aaucugaaag cugacuacug ucuugaaauu     1440

cugggcuacu cucccaagga gugggaaaug aacuauagcg uucucgaggg gaauagaacg     1500

aacaaagccc uguacgaagc cuaucugaag aucacagauc uugagggaua cgacgcuaag     1560

aaccaccuca aagugaagcu gaauaaagau gagguggaac uggacgaucu caaggucccu     1620

gccuccgaga uuaaagaaau ggugcaggcg aucuuuaagg aggcuggcau caacaccgac     1680

auucugcauu ucaaugccga acuugacggg aaggauuuug agaaccaggc cucuuaucag     1740

cuguggcacc uccuguacag cuaugaggga gacgauucca aaagcggcaa ugaacugcuu     1800

uacaagcucc uggagaccaa auucggcuuu aagaaagaau acgcucaaau ccuggccaac     1860

guuucucucu ccgacgauua ugggaaucug agcacaaagg cuaugcgcaa gauuuacccg     1920

uauaucaaag agaacaaguu cucuaccgcc ugcgagcuug cgggauaccg gcacuccaaa     1980

cauagccuga caaaggaaga gaaugacaac agagugcuca aagauucccu ggaaguccug     2040

aagaaaaauu cucuucgcca gcccgugguu gagaagauuc ucaaccagau gaucaaucug     2100

gugaacgccc ugcucaagaa auaucggacg gaaaauccua acuuuaaguu cgacgagauu     2160

agaaucgagc uggcucgcga acuuaaaaag aacgccgagg aacgggcuaa uaugaccgcc     2220

aacaucaaua aagcgaagac agagcacgaa aagauuauca aaauucugaa gaacgaguuu     2280

ggccucccca auccuaccag aaacgauauc auucgcuacc ggcuguauga ggaacugaaa     2340

gccaaugggu acaaggaccu uuacacaaac aucaaaaucc cccaagagaa gcuguucacc     2400

aaggaaauug auaucgagca uauuaucccg aaaagcagag ucuucgacga uuccuuuucu     2460

aauaagacgc ucagcuuccg gaaaaccaac cuggacaagg gagaacgcac agcuuuugac     2520

uauauggagu ccaaauucgg cgccgauaag cucgaggaau acguggcuag agucgaggac     2580

cuguauaaga auaacauuau cagcaaagcc aaguaccaga aacuucugaa gaaagaaucu     2640

gagaucgggg auggcuuugu ggagcgggac cucagagaaa cccaguauau ugccaagaag     2700

gcuaaagaga uccugcugca gauuacacgc aauguucuuu ccaccagcgg aggcaucacg     2760

gaucggcuga gagaagacug ggaucucgug aacgucauga aggagcugaa ucucccuaaa     2820

uaccgcgcgc uggggcuuac agaaauggag gacagaaagu auggacaaca gguggagguu     2880

auuaucgauu ggaccaaacg gaacgaccac cgccaccaug ccauggaugc ucugacagug     2940

gccuucacca agcacaacca uauccaguac cucaauuacc ugaacgccag aaagaaugaa     3000

aagcaccggg agcauucuaa cauuaugggc auccagcaac uggaaaccau uaaggucaca     3060

gacaaaaaug ggaacgagaa gcgcguguuu aaagcuccca ugccuaauuu cagacaggug     3120

acgaaggccu uucuugaaaa cguucugauc ucccacaaag cgaagaauaa ggucgugacc     3180

aaaaacaaga auaaaguggc uggaagcaac aagguccagg aggugcucac accccggggc     3240

cagcugcaca aagagaccgu uuaugggaag uaccaauauu acgugcagaa ggaagagaaa     3300

auuggcggaa aguucgaucu cgaaacaauc aaaaaggucg ccaauccccu guaucagucu     3360

cuucugaaaa agcgccugga ggaaaacggc ggggacccua agaaagccuu uaccggaaag     3420

aauagccucg cuaaaaaccc gauuuaccug gacgaugccc aaacggagca gcuucccgag     3480

aaggugaaac ugguuuggcu cgaaaauucc uucagcauca gaaaggacau cacaccugag     3540

aacuuuaaag augaaaagcu gauugacaag auccucgaug agggcgugaa acggauucug     3600

aaggagcgcc uucagucuuu cgggaacgac gcgaaaaagg cuuucuccga ucuggacaaa     3660

aaucccaucu ggcugaacaa ggaaaaggga auuaccauca aaagagucac caucagcggc     3720

guggccaaug ccgagccucu ccauacaaag aaagaucacc uggggaagga aauucuugac     3780

aaagagggca agcccauccc gguugauuuu auuuccaccg gaucuaacca ucacguggcu     3840

gucuaucggg acgaaaaggg caaucugcag gagaaagugg ugagcgucuu cgaggccguu     3900

gugagagcua accaagggcu cccuaucauu gacaagacau acaaugaaca ccugggaugg     3960

gaguuucucu ucacgaugaa acagaacgaa auguuugugu uccccaauga guccaccggc     4020

uuuaacccua augauaucga ccugcuugau cccaagaaca aacgcaucau uucuccuaau     4080

cuguuccggg uccagacaau cagcgugguu aaguacggga acgccauugu gagagacuuu     4140

aaguuccgcc aucaccugga aaccacaguc gaggauaaaa aggagcucca gggaaaaacc     4200

uaucaacaga ucaagucccu gucuccccuu aaugacauua ucaaagugcg gauuaaccau     4260

cugggcgaua ucguuagcgu gggggaauac uga                                  4293


<210>  4508
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4508
auggcccaga aguacauccu gggccucgac cuggggacca acagcauugg augggcugug       60

gucaaagagg ccgaaaauga uaacgagcaa uccgaaaucg uugacguggg cgugagaguc      120

aauccccuua caacggauga gaagaccaac uucgaaaaag ggaagccuau uucuacaaau      180

gcggaccgga cccugaaacg cggagcuaga cggaaccucc agcgcuauaa gcugagacgg      240

aaggagcuua ucaaaauucu gaaggagcac cagcucauca gcgaugacac aauccuggcc      300

gaaaauggca aagggaccac auaccagacc cugaagcucc gcgccaaauc cucuaaggag      360

aagauuagcc uggaggaacu ugcuagagug uuucugacga ucaacaaaaa gcggggauau      420

aaauccucuc gcaagaccaa aaaugaggau gaaggccaac ucauugacgg cauggagauc      480

gccaagagac uguacgaaga gaaccuuaca accgggcagc ugagccucca gcugcugaaa      540

gaaggaaaga agucccuccc ggauuauuac cggagcgacc uggagcuuga gcuggauaaa      600

guuuggaauu uccagaagca auuucauucu gacauucuca cagaugaguu cuauacccag      660

cugaaaggca aggggcagcg cgcuacgucc gcccuuuuuc uggaaaaaua ccagaucuac      720

acaguggaaa acaagggaaa uagagaggaa aagaaccucc aagcguauca guggcggguc      780

gacgcccuga gcaaagagcu gacccgcgag gaacucgcuu ucgugauuac agagaucaau      840

aacaaccuga auaacucuuc cggcuaccuu ggggccauca gcgacagauc uaaggaacug      900

uauuuuaaua acgagaccgu uggagaauac cucuggaaac agauugcugc caauccccac      960

accucccuga agaaccaagu guucuaucgg caggauuacc uugacgaguu ugagagaauc     1020

uggaauacac aggcgcaguu cuauccucaa auucugacgg aaagccucaa aaaggagcug     1080

cgcgaugucg ugaucuuuua ccagcggaag cugaaauccc agaagucucu cgugagcuuc     1140

ugcgaauuug agaacaaaga gauugaaauc aagaaugagg acggcaccac aaccaaaaag     1200

gucaucggcc ugaagguugc ccccaaaucc agcccucuuu uccaggaguu caagauuugg     1260

caaaaccuga auaacgugcu caucagaaag aaggggucua aaacagaaaa gguggcuaaa     1320

aaucagcagg ucgugcuguu ugaagaggag aagaccauuu ccgaacugga ucuugaggcc     1380

aagcaggcuc ucuucaacga acugaaucug aaaggaaacc ucaaggccga cuacugucug     1440

gagauccuug gcuauagcuc uaaagaaugg gagaugaacu acagcguucu ggaggggaau     1500

cgcacgaaca aggcccucua ugaggcuuac cugaaaauua cagaucugga aggauaugac     1560

gcgaagaauu accuuaaggu gaaauccaac aaggaugaag ucgagcucga cgaucugaaa     1620

gugcccgccu cugaaaucaa ggagaugguu caagcuaucu uuaaagaggc cggcauuaau     1680

accgacaucc ugcauuucga cgccgaacuc gaugggaagg acuuugagaa ccagggaagc     1740

uauaagcugu ggcaccuucu guacuccuac gaaggcgaug acagcaaauc uggcaaugag     1800

cuccuguaua agcugcuuga gaccaaauuc gggcucaaga aagaauacgc ucagauucug     1860

gccaacaucu cccugagcga ugacuaugga aaucucucua caaaggcgau gcggaagauu     1920

cauccuuaca ucaaagagaa caaguuuucc accgcuugcg aacuggccgg cuauagacac     1980

agcaaacacu cccuuacaaa ggaggaaaau gagaaacgcg ugcugaagga ugggcucgag     2040

guccugaaaa agaacucucu gcggcagccg guggucgaaa agauucuuaa ucaaaugauc     2100

aaccucguga augcccugcu gaaaaaguac agaacggaga accccaacuu caaauuugac     2160

gaaauccgca uugagcucgc ucgggaacug aagaaaaaug ccgaggagag agcuaacaug     2220

accgccaaua ucaacaaggc gaagacagaa caugagaaaa uugauaagau ccuuaaaagc     2280

gaguucggac ugccuaaucc cacccgcaac gacauuaucc gguauagacu cuacgaagaa     2340

cugaaggcca auggcuacaa agaucuguau acaaaccggu acaucuccuc ugagaaacuu     2400

uuuagcaagg agauugacau cgaacacauu aucccuaaau cccgcguuuu cgaugacagc     2460

uuuucuaaua agaccauugu guauagaacg gacaaccugg auaaagggga gcagaccgcu     2520

uacgacuaua ucgaauccaa guucggcgcc gagaaacuca aggaguacau gacacggguc     2580

aaggaucugu aucagaaaaa gaucauuagc aaagcuaagu accaaaaacu ccugaagaag     2640

gaaucugaga ucggagacgg cuucguggaa agagaucuuc gcgagaccca guacauugcc     2700

aaaaaggcga aagaaauccu gcuccagauu acacauaaug uucuguccac cagcggggga     2760

aucacggacc ggcugagaaa ggauuggggc cuugugaacg ucaugaaaga gcugaaucuc     2820

cccaaguauc gcgcccuggg gcucacaaaa auggaggaca gaaaguacgg acagcaagug     2880

gaagugauca ccgauuggac aaagcggaac gaccugcgcc accacgcuau ggaugcccuu     2940

accguugcuu uuaccaaaca uaaucacauu caguaucuga acuaccucaa cgccagaaag     3000

aaugagaaac auaaggaaca caacaauauc auggccauuc agcagcugga gacaaucaaa     3060

gucacggacg aaaacggcaa ugagaagcgg auuuucaagg cuccgaugcc uaacuuucgc     3120

caaguggcga aaaccuuccu ggagaaugug cuuaucucuc auaaggccaa aaacaagguc     3180

gugaccaaaa auaagaacaa gauuagcggg uccaauaaag uucaggaagu gcugaccccc     3240

agaggccagc uccacaagga gacagucuau ggaaaauacc aguauuacgu gcaaaaggaa     3300

gagaaaaucg gcgcuaaguu ugauagcgaa accaucgaga agguugccaa cccucuguac     3360

cagucucucc ugaaaaagcg gcuugaggaa aaugggggag accccaaaaa ggccuucacg     3420

ggcaaaaacu cccuggcuaa gaaucccauu uaucuggacg auacacagac cgagcaacuc     3480

ccugaaaaag ugaagcuggu cuggcuugag aacagcuuuu ccauccgcaa ggacauuacc     3540

ccggagaauu ucaaagauga aaagcugauc gacaaaauuc ucgaugaggg ggugaagaga     3600

auccugaaag aacggcucca gucuuuugga aacgacgcca agaaggcguu cagcgaucug     3660

gagaaaaacc ccaucuggcu uaauaaggaa aaaggcauug cuaucaagcg cguuacaauu     3720

uccggggugg ccaacgccga gccucugcac accaaaaagg accaucuggg caaggagauc     3780

cucgauaaag aaggaaagcc cauuccuguc gacuuuaucu cuacaggcaa uaaccaccau     3840

guggcugugu acagagauga gaaagggaau cugcaggaaa agguccuuag cguuuucgag     3900

gccguggugc gggcgaacca gggacugccc aucauugaga aaacguauaa ucaacaccuc     3960

ggcugggaau uucuguucuc caugaagcag aacgagaugu ucgucuuucc gaaugaaucu     4020

accggguuca acccuaauga caucgaccuc cuggauccca agaacaaaag aauuaucagc     4080

ccuaaucuuu uuaaggugca gaaacuggcu acaaagaacu acauguucac ccaccaucug     4140

gagacaaaau ccauugacgg agauaugcuc aagaauaaaa aggaacugag cggcauccuu     4200

uauaacuuua uucagacacc ccaaaagcug uacgggauca ucaaaguucg cauuaaccac     4260

cucggcgaca ucgugucugu cggagaguau uga                                  4293


<210>  4509
<211>  4296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4509
augaagagaa uccugggccu cgaccugggg agcuccucua uuggaugggc ccuugugaac       60

gaggcugaaa gcaaaaauga gcgguccucu aucgucaagc ugggcguucg cgugaacccc      120

cucaccgugg augaaagcau uaauuucgag aaagggaagu ccaucacaac gaacgccgac      180

agaacccuga aacggucuau gcgcagaaau cuucaccggu acaagcugcg cagagaagcg      240

cucauugagg uccugaagga gugcaaauuu aucacagaag auaccauccu gagcgagaac      300

ggaaaucgga caaccuucga aacauaucgc cucagaguga aggcucugac cgaggagauu      360

ucccuugaag aguuugcccg gguucugauc augauuaaca aaaagcgcgg cuacaaaagc      420

ucuagaaagg ccgggggagc ugaagaggac acgcucaucg auggcaugga cauugcccgg      480

aagcuguaug augcuaaucu uaccccuggc gaacuguguc uccagcugcu guccgccggg      540

aaaaaggugc ucccggacuu cuaccgcagc gaucugcaaa acgagcuuga cagaaucugg      600

gagaaacaga aggaauauua ccccggcauu cugacagcgg cccucaaaga ggaacugcgg      660

ggaaagaaaa gagaugcugu cugggccauc ugcgcuaaug ccuuugugug gaaggagacc      720

uauacagaau ggaacaauga ggagucuaag aacgaacaga ucgagaaaga acauaaacuu      780

gaggggauuu acuccaagcg caagcgggac gaagcgaaaa aggagaaucu gcaguggaga      840

guuagcggac ucaaggagaa acugucucug gaacaacucg ugaucauucu gcaggagauc      900

aacacccaga uuaacaauuc cagcggcuac cuugggacga ucuccgaucg cucuaaggaa      960

cuguauuuca acaaacagac agucggacaa uaccucaugg acauccugga caauaacccu     1020

aaugccagcc uucggaacau uguguuuuau agacaggauu accuggacga guucaaugug     1080

cucugggaga agcaggcuga auuucacaaa gagcugaccg gcgaacugaa gaaggagauc     1140

cgcgauauua ucauuuucua ucagcggaga cucaaauccc aaaagggccu gaucagcuuu     1200

ugugaguucg agucucgcca gaucgagguc aaaauugacg ggaagcggaa aaucagaaca     1260

guuggaaacc gcgugauucc ccgguccagc ccucuuuuuc aggaguucaa gaucuggcaa     1320

auucugaaua acaucgaagu gaccgccguc ggcaauaaga acaaacagag aaugcagaau     1380

gggucuaacg aacccgauga caccgagcag cucgaacuga auggacggcg cuaccuguau     1440

caagaggaga aggaacuucu cgcuaaagag cuguucguga gagauaagau gacaaaacug     1500

gacguucucc ggcugcuuuu ugauaacccu gaagaacugg accucaacuu caagacgauu     1560

gauggcaaua aaaccgggua ccuguuuuuc caggccuaca gcaagaugau cgaaaugucc     1620

ggacaugagc cgguggacuu uaagaaaccc aucgagaaga uugucgaaca ggugaaaucu     1680

guuuucgugc ugcuuaacug gaauacagac auccucagcu uugauuccaa caaggagcug     1740

gacaaacaga gcuauuacaa gcuguggcac cuccuguauu cuuucgaagg cgauaauacc     1800

ccuacaggca acgggugccu uguccaaaag auuauggacu ccuacggacu gggcaaagag     1860

uuugccaaga uccucgcuaa uauuaccuuc caggaggauu augggagccu gucugcgaaa     1920

gccauccaua agauccugcc ccaccuuaaa gaaggaaacc aguacgacgu ggcuugugag     1980

uaugccggcu acagacacuc caagagcucc cucacgaagg aagagauugc caauaaaguc     2040

cugaaggaua aacuggaagu gcucccucgc aacucucugc ggaaucccgu uguggagaag     2100

auccuuaacc agauggucaa ugugauuaac gcuaucauug aggccuacgg gagaccggac     2160

gaaauccgcg uugagcuggc gagagaacuc aaaaagaaug cuaaggagcg ggaaaaacug     2220

acaaagagca ucgccgagac caccaaaaca caugaggccc acaagaaacu gcuucaagaa     2280

gaguuuggcc ucaagaacgu gggacgcucc gauauucuga gauauaaacu guacaaggaa     2340

cucgagucuc ggggcuauaa gacccuguac agcaacacau auaucccucg cgaaaaacuu     2400

uucuccaagg aguuugacau ugagcauauc auuccccagg cuagacuguu cgaugacagc     2460

uucucuaaua aaacgcucga agcccggaac gucaauaucg agaaggggaa ccgcaccgcu     2520

uacgauuuug ugaaagaaaa guucggagag uccggcacag acaauagccu ggacugcuau     2580

cugaacaaua ucgaggugcu uuuuguuucu gggaagauuu ccaaaaccaa guacacaaaa     2640

cugaagauga ccgaacagga uaucccugga ggcuucauug agagagaccu ccggaacacg     2700

caguacaucg ccaaaaaggc gcugagcaug cucaaugaaa uuucucgcug ugucguggcc     2760

accagcgggu ccaucacaga uaagcugaga gaggacuggc aacuugugga ugucaugaaa     2820

gaacugaacu gggagaagua uaaagcucuc ggccuggugg aguacuuuga agacaaggau     2880

ggaaaacaga uuggccggau caaggacugg agcaagagaa augaucaccg ccaccaugcc     2940

auggacgcuc ugaccguugc cuucacaaaa gagguguuua uccaguauuu caacaauaag     3000

aacgcgucuc uuaaucccaa caccaacgaa uacgccauua aaaauaagua uuuucaaaac     3060

gggcgggcua ucgcccccau uccucugaau gaguucagag cugaagccaa aaagcaccuc     3120

gagaacacgc ugaucuccau uaaggccaaa aauaagguca ucacaaacaa uaucaacaaa     3180

accaagaaac gcggaggcgu gaauacaaag aaacagcaga ccccgcgggg gcagcuccau     3240

cuggagacca uuuacggaug cuguagacaa uaucuuacaa aggaagagaa gguuaacgcu     3300

agcuuugaug uguccaaaau cgcgacgguc ucuagcuccg uguaccgcga cgcccugcuc     3360

aagcggcugc aggaacacca uaaugauccc aaaaaggcuu ucgccggcag aaacucucug     3420

gacaaaaagc cuguuuggcu ugacaaggag cagauucgca aagugcccga aaaggucaaa     3480

accgugacau uugagaccau cuacacaauu cggaaggaga ucagcccuga ucugagagug     3540

gacaaaauca uugauuccgg gguccggaag augcucaccg gccgccugaa ggaauaugga     3600

aaugacgcca aaaaggcuuu cucuaaccuc gaugagaaac ccaucuggcu gaauaaggaa     3660

aaaggcauuc cgaucaagag aguuacgauu agcgggaucu ccaacgccca gagccuucac     3720

gugaagaaag acaaggaugg aaaaccugug cuggacggca augggcggaa cauucccguc     3780

gauuucguga acacaggaaa uaaccaccau guugcggugu accgcagacc ugucaucgac     3840

aagaauggcc aaauggugau cgaugagaac gggaaucuga aauaugagcu cgaagagguu     3900

guggucaccu uuuucgacgc ugugacccgg guuaaccugg gccagcccau uaucgacaag     3960

gauuacaaag ccucugaagg auggcaguuu cuuuucucca ugaagcagaa ugaguauuuu     4020

guguucccua acaagaaaac aggcuuuaau cccaaggaaa uugaccugcu cgaugccgac     4080

aacuacgggc ugaucagccc gaaucucuuc agaguccaaa aauuuaccca caagaacuau     4140

guguuccgcc aucaccugga gacaacgauu aaagauaccu cuuccauccu ucggggaauc     4200

acauggauug acuuuagaag cucuaagggc cuggauucca ucgugaaggu ccgcguuaau     4260

cacauugggc agaucgugag cgugggagag uacuga                               4296


<210>  4510
<211>  4299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4510
augaagaaaa uccugggccu cgaccugggg agcaacucca uuggaugggc ccuugugaau       60

gaggcugaaa acagagauga ggccucuagc aucauuaagc ugggcguccg gguuaauccc      120

cucaccgugg acgaaaugca gaacuucgag aaagggaagu ccaucacaac gaaugcggau      180

cgcacccuga aaagaucuau gcggcgcaac cuucaaagau acaagcugcg gcgcgacgcu      240

cucauuaagc ugcugaaaaa ggugggauuu aucgccgaug gcacaauccu cagcgaacag      300

gggaauagau ccaccuucga gacauaucgg cugcgcgcca aagcuccuga cgaggaaauu      360

ucucuucagg agcuggccag aguccuccug augaucaaca agaaacgggg auacaagagc      420

ucccgcaaga uuaaagaaaa ggaggcuggc acccuuaucg auggcaugaa aguggccaag      480

aaacuguaug acgaggaucu cacaccgggg cagcugugcc ugcaacuccu ggaaagcgga      540

aagaaaucuc uucccgacuu uuacuccagc gaucugcagg aggaguucga cagaauuugg      600

aauuuucaga agcaguucaa cccuaaggcg uuuugugaua ccgccaaaga ggaaaucaag      660

ggcaaaaaua agucucaaac gugggcuauu cucgccaaau auuucaucug gaccgacgag      720

augguugugu ggaacgagaa ggaauccaag acagagcggg ucgaaaaaga gugcaagcug      780

guggggcuua aacgcaccgu uaagaaagaa gaacagagaa aggagaauua ccgguggaga      840

gugcaggcuc ugacagaaca gcucgaccug ggaaaggucg ccguggugcu gcaagagauc      900

aacggccaga uuagcaccuc uuccggguau cucggagcga ucagcgaucg cuccaaaguc      960

cuguacuuua aucggcagac gguuggccaa uaccagaugg ccgaacuuga gaagaacccc     1020

aacacaucuc ugagaaauau gguguucuau cgccaggacu accucgauga auuugacaaa     1080

auuugggaga agcaggcuga guuccacaaa gaacugaccg uggagcuuaa gaaggaaauc     1140

cgggauauua ucaucuuuua ucaaagacgc cugaaaagcc agaagggccu cauuggguuc     1200

ugugaguucg agaaccggga aaucaugguc gagaaaaaug gaaagaugca ggccaaagug     1260

guuggcugca aggugauucc uagaucccau ccccuguuuc aggaguucaa aaucuggcaa     1320

acacugaaca augucgaagu guuugcuggg gaaaagaagu guaaacgcaa gcggaccgac     1380

gccuucccug gacucuuuga uggcaccgag gacacacuga ugguugaggg guggagauac     1440

cuuuaucagg aagagaaaga aauucuggcc aaggagcuca gcgugaaaaa gucucugaag     1500

aaagaauaca uccugaaacu ucucuucgau aacccgcagg accuggauau gaauuuugag     1560

cagaucgagg gaaacgacac gggcaauguc cuguucuccg cuuauagcaa aaugauugaa     1620

aaguacggcu acgagccccu cgacuuuggg aaaucuaccg augaaaucau ugagcgccug     1680

caagugaucu ucacagaccu uggauggaac accgaucugc ucuuugucga ccugaauaag     1740

gaaggcaagg aucuggagaa acagccuuau uucaaacuuu ggcaccuccu guacagcuuu     1800

gagggggaca acacacccac cggaaauggc aagcuggugg aaaagcucau ggagcugugc     1860

cauguuggga aagaaugugc gcuugagcug gugaacauuu ccuuccagaa ugauuauggc     1920

ucucucagcg ccaaggcuau caagaaaauu cugccuuacc ugaaggaggg aaaccgguau     1980

gacgucgccu gcggcuacgc cggguauaga cacuccaaaa gcucucuuac gaaggaagag     2040

aucgaaaaca aagugcucaa ggauaaacug gacauccugc ccaaaaauuc ccuccggaac     2100

ccgguuauug agaagauccu gaaucagaug gugaacguca uuaaugcuau cauugauaca     2160

uacggaaagc cugacgaaau ccgcguggag cuugccagag agcugaagaa aagcgcgaag     2220

gaacgggaug agcucaccaa agcuaucgcc aagucuacca gagaacauga ggaaauucgc     2280

aaggugcugc aagaggaguu cggcaugauc aacguuuccc ggaaugacau uaucagauac     2340

aaacuguaug aggaacuuaa ggacaacggg uacaaaacac ucuauagcaa ucaguacauu     2400

cccaaggaaa aagucuuuuc caaggagauc gauaucgaac acauuauccc ucaggcccgc     2460

cuguucgacg auucucugag caacaagacc cucgaguaua aagcuauuaa uaucgagaag     2520

ggaaacaaaa cagccuacga cuuugugaag gaaaaauaug gcgagaaggg gcugcaacag     2580

uaccuuaaua gaugugaauc ccuguucaag ggccaccgga cgaaacuccg caagcugaaa     2640

augggacagg agaacauucc cgagggcuuu aucgauagag accugcggaa uacccaguac     2700

aucgcuaaga aagcccuugc gaugcugaac gaaauuugcc gcagaguggu cgccacaacc     2760

gggucuauca cagaucggcu ccgcgaggac uggcaacugg uggauguucu caaggaacug     2820

aaccuuccca aguaugagac ccugggacuc guggaagucu acgaggacaa agauggcaga     2880

aagauuaaac ggaucaagga cuggacgaaa cgcaaugauc auagacacca ugcuauggac     2940

gcccugaccg uggcuuucac aaaggacguu uuuauucagu auuucaacaa uaaaaacgcc     3000

gcguggcagg ccgauuguaa ggagcacagc gacaucauug ggaucaagac ccgguacuuu     3060

gaaaauggca gagcucugcc uccgaugccc cuugugcgcu uccgggccga ggcuaaaucc     3120

caccuggaaa cacuccuggu cagcaucaag gccaaaaaca aggugguuac caauaacgug     3180

aauugcacga aaagaaaggg aggggagaac aagaaacucc agcaaacacc ucgcggccag     3240

cugcaucuug aaaccauuua uggaucucac cggcaguacg ucaugaagga ggagaaagug     3300

aaugccuccu uugauagcuc uaagaucgcu acagugugca aacaggcgua uagaaacgcc     3360

cugcucgaac gccuggcugc cuucggcaau gaccccaaga aggccuuuac cgggaaaaac     3420

ucccuggaga agaauccugu cuaccuugau cgggaacaaa ccgcuagagu ucccgagcgc     3480

gugcggacag uggaacugga gacggucuac accauuagaa aaccggugga cccuaaccuc     3540

agcguugaua agguggucga cgccaaagug cggucuaucc uggagcagcg ccucagagaa     3600

uaugauggag aggcgaagcg ggcuuucguu aaccuggacg aaaaucccau uuggcuuaac     3660

gaggagaagg gcaucgccau caaacgcgug acaauuagag ggaucaauaa gguccagagc     3720

cugcauggaa agaaggauaa aaacggcaau cugauucucg acgaaaccgg gaagaaaauc     3780

ccuguggacu ucguuaacac aggcaauaac caccaugugg ccauuuaccg gaagcccguc     3840

cuggauaaga ccggacaaga gaugcuugac aaugaaggca acccugugua ugagcuggau     3900

gaaguggucg uuuccuuuuu cgaggcugug acgagaguga aucucgggcu gcccgucauc     3960

gacaaaaacu acaauaagag cgagggaugg caguuuaugu ucacaaugaa acagaacgaa     4020

uauuuugugu ucccgaauga gaagaccggc uuugauccua aagaaguuga ccuccugaac     4080

cccgagaauu acgccgugau cucucccaac cuuuuccgcg uccagaagau uaccgaaaag     4140

gauuauaugu uucggcacca ccuggagaca aaagugguug acgauaaguu ccugaaagag     4200

aucaccugga agcgcauucu cacaccuaac aaacuggaag ggaucgugaa ggucagagug     4260

aaucauauug gacaaaucgu cuccgugggc gaguacuga                            4299


<210>  4511
<211>  4299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4511
augaagaaaa uccugggccu cgaccugggg agcaacucca uuggaugggc ccuugugaau       60

gaggcugaaa acucugauga ggucagcucc aucauuaagc ugggcguuag agugaauccc      120

cucaccgugg acgaaaugca gaacuucgag aaagggaagu cuaucacaac gaaugccgau      180

cggacccuga aacgcagcau gagacggaac cuucaacgcu acaagcugag acgggacgcg      240

cucauuaagc ugcugaaaaa ggucggauuu aucacagaug gcaccauccu cuccgaacag      300

gggaaucgca caaccuucga gacauauaga cugcgggcua aagccccuga cgaggaaauu      360

ucucuucagg agcuggcccg cgugcuccug augaucaaca agaaaagagg auacaagagc      420

ucccggaaga uuaaagaaaa ggaggcuggc acccuuaucg auggcaugga gguugccaaa      480

aagcuguaug acgaagaucu cacgccgggg cagcugugcc ugcaacuccu ggagagcgga      540

aagaagucuc uucccgacuu uuacuccagc gaucugcagg aagaguucga ccgcauuugg      600

aauuuucaga aacaguucaa cccuaaggcu uuuugugaua ccgccaagga agagaucaaa      660

ggcaagaaua aaucucaaac augggcgauu cucgccaagu auuucaucug gaccgacgag      720

augguggucu ggaacgaaaa agaguccaag acagaaagag uggagaagga augcaaacug      780

guugggcuua agcggaccgu gaaaaaggag gagcagcgca aagaaaauua cuguuggaga      840

guccaggcuc ugacggagca gcucgaccug gaaaaggugg ccgugguccu gcaagagauc      900

aacggacaga uuagcacauc uuccggcuau cucggggcua ucagcgaucg guccaagguu      960

cuguacuuua auagacagac cgugggacaa uaccagaugg ccgaacuuga gaaaaacccc     1020

aacacaucuc ugcgcaauau gguguucuau cggcaggacu accucgauga guuugacaag     1080

auuugggaaa aacaggcgga guuccacaag gaacugaccg ucgagcuuaa aaaggagauc     1140

agagauauua ucaucuuuua ucaacgccgg cuuaagagcc agaaaggccu cauuggcuuc     1200

ugcgaguucg agaacagaga aaucauggug gagaagaaug ggaaaaugca ggccaagguu     1260

gugggaugua aagucauucc ucgcucccau ccccuguuuc aggaguucaa gaucuggcaa     1320

acccugaaca auguggaagu uuuugcuggc gagaaaaagu gcaagcggaa aagaacagac     1380

gccuucccug ggcucuuuga uggaacggaa gacacccuga ugguggaggg cuggcgcuac     1440

cuuuaucagg aagagaagga aauucuggcu aaagagcuca gcgucaagaa aucucugaag     1500

aaggaguaca uccugaaacu ucucuucgau aacccgcagg accuggauau gaauuuugaa     1560

cagaucgagg ggaacgacac aggaaaugug cuguucuccg ccuauagcaa gaugauugaa     1620

aaauacggcu acgagccccu cgacuuuggc aagccuaccg auaaaaucau ugaacaacug     1680

caggucaucu ucgccgaccu uggguggaac acagaucugc ucuuugugga ccugaauaag     1740

gagggaaagg aucuggagaa acagcccuau uucaagcugu ggcaccuccu guacucuuuu     1800

gaaggcgaca acaccccuac ggggaaugga aaacugguug agaagcucau ggaacugugu     1860

caugugggca aagagugcgc ucuugagcug gucaacauua gcuuccagaa ugauuauggg     1920

ucccucucug cgaaggccau caagaaaauu cugcccuacc ugaaggaagg caaccgguau     1980

gacguggcuu guggauacgc cggcuauaga cacagcaaau ccagccuuac aaaggaggaa     2040

aucgagaaca aaguucucaa ggauaaacug gacauccugc cgaaaaauuc ucuccgcaac     2100

ccuguggucg aaaagauucu gaaucaaaug gugaacguga ucaaugccau uaucgauacc     2160

uacgggaaac ccgacgagau ucggguugag cuugcuagag aacugaagaa auccgccaag     2220

gagcgggaag aacucaccaa agcgaucgcu aagagcacac gcgaacauga ggagaucaga     2280

aagguccugc aggaagaguu cggaaugauu aacgugucuc ggaaugauau cauuagauac     2340

aaacuguaug aggaacuuaa ggacaacggc uacaaaaccc ucuauuccaa ucaguacauc     2400

ccuaaggaaa aaguguuuag caaggagauu gacaucgagc acaucauucc ccaagcccgc     2460

cuguucgaug acucccuguc uaacaagaca cucgaauaua aagccaucaa uauugagaag     2520

gggaacaaaa cggcuuacga uuuugucaag gaaaaauaug gagagaaggg ccugcagcag     2580

uaccuuaauc ggugcgagag ccuguucaag gggcagagaa ccaaacuccg caagcugaaa     2640

augggccaag aaaacauccc cgagggauuu auugacagag aucugcggaa uacacaguac     2700

aucgccaaga aagcucuugc caugcugaac gaaaucuguc gcagaguggu ugcgaccaca     2760

ggcuccauua ccgaccggcu ccgcgaggau uggcagcugg uggacguccu caaggaacug     2820

aaccuuccua aguaugagac gcuggggcuc guggagguuu acgaagauaa agacggaaga     2880

aagaucaaac ggauuaagga uuggaccaaa cgcaaugacc acagacauca cgccauggau     2940

gcucugacag uggccuucac caaggacguc uuuauccagu auuucaacaa uaaaaacgcu     3000

gccuggcaag cggacugcaa ggagcauucu gauauuaucg gcauuaagac acgguacuuu     3060

gaaaaugggc gcagccugcc gcccaugccu cuugugagau uccgggccga ggcuaaaucc     3120

caccuggaaa cccuccuggu uagcaucaag gccaaaaaca agguggucac gaauaacgug     3180

aauugcacaa aaagaaaggg aggcgagaac aagaaacucc agcagucucc ccgcgggcag     3240

cugcaccuug agaccaucua uggcucccau cggcaauacg ugacaaagga agagaaaguc     3300

aaugcuagcu uugacucuuc caagauugcc accguuugua aacaggccua uagaaacgcu     3360

cugcucgaac gccuggcggc cuucggaaau gauccuaaga aggcuuuuac cggcaaaaac     3420

agccuggaga agaaucccgu guaccuuaac cgggaacaga cagccagagu gccggagcgc     3480

guccggacgg uggagcugga aaccguuuac acaaucagaa aaccugugga ccccaaccuc     3540

ucugucgaua aggugguuga cgccaaagug cgcagcauuc uggagcaacg gcucagagaa     3600

uaugaugggg aggcuaagcg ggccuucguc aaucuggacg agaacccuau cuggcuuaau     3660

gaagagaagg gaauugcgau caaacgcgug accaucagag gcauuaacaa gguucagucc     3720

cugcacggga aaaaggauaa aaauggaaac cugauccucg acgaaacagg caagaaaauu     3780

cccguggauu ucgucaauac cgggaacaau caucacgugg cuaucuaccg gaagccugug     3840

cugaacaaga cgggccagga gaugcuugac aaugaaggaa accccgucua ugagcuggac     3900

gagguugugg ugagcuuuuu cgaagccguc acacgcguga aucucggccu gccgguuauu     3960

gauaaaaacu acaauaaguc ugaggggugg caguuuaugu ucaccaugaa acaaaacgaa     4020

uauuuugugu ucccuaauga gaagaccaga uuugacccca aagaagucga ucuccugaac     4080

cccgagaacu acgccgugau cuccccuaau cuuuuccggg uucagaagau cacagagaag     4140

gacuauaugu uuagacauca ccuggaaacc aaaguggucg augacaaguu ccugaaagag     4200

auuacaugga agcgcauccu cacgccgaac aaacuggaag gaauugugaa gguccgggug     4260

aaucacaucg gccagauugu uagcgugggg gaguacuga                            4299


<210>  4512
<211>  4299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4512
augaagaaca uccugggccu cgaccugggg accacaagca uuggauucgc ccacaucgug       60

gaggaugaaa auaaagagaa guccgaaauu aaagagcuug gcgucagaau cguuucucug      120

acgaccgacg aacagagcga uuuugagaag gggaaaucca uuacaaccaa cgcuaaucgg      180

acacucaagc auggagcccg ccugaaccuu gacagauacc aacagcggcg caaguaucug      240

aucgaucucc ugcacaaagc gaaucugauc acccccucua gcauucucgc ugagaacggc      300

aagaauacaa cccauuccac guggcagcug agagccaaag ccgugaccga acggaucgag      360

aaggaagagu ucgcucgcau ucuucuggcc aucaacaaaa agagagggua caagucuagc      420

cggaaagcua agacagagga cgaaggacag gccauugaug gcauggcgau cgccaaacgc      480

cucuaugacg agaaucugac cccuggccaa cuuucccugc agcuccugca gcagaacaag      540

aaacugcucc cggauuuuua cagaagcgac cugcaaaagg aguucgaucu uguguggaau      600

uuucagaaac aguucuaucc cgacauucug acagauaucu uuuacaagga gcuccagggg      660

aagggaaaag acgcuaccuc uaaggccuuc uccaaacggu aucacuuuga cacggcugaa      720

aacaagggca gcaaagaguc uguccgccug caagccuacc aguggagagc ggaggccgug      780

uccaagcagc uuagcaagga agagguugcu uacgugcuga cagaaaucaa uaacaaucuc      840

aacaacgccu cuggguaucu gggagcuauu uccgaucgga gcaaagagcu guacuucaau      900

cgccaaaccg ucggccagua ucucuacgcc cagcugcagg aaaacagaca uaauucccuu      960

aagaacaaag uguuuuaucg gcaagacuac cuggaugagu ucgagagaau cugggaaaca     1020

caggcccucu uucacaagga gcugaccgac gaacuuaaaa agcagauucg cgauguugug     1080

aucuucuauc agcggaagcc uaaaucucaa aaggggcuga uuagcuucug cgaguuugaa     1140

uccaaagaga ucgagaucga aaaggacgga aaaaccauua caaagaauau cggcgcuaga     1200

gucgugccca agagcucucc ucucuuccag gaguuuaaaa uuuggcagau ccugaacaau     1260

gugauuugua agcgcaaagg cauccggaag aaaaagaucu ccgcgaaaac gacccagcug     1320

gaucuccuga acgaaagcuc ucaaacaauu uucagccuug acauggagug caagcagcug     1380

cucuuugaug agcugaaucu gaagggggac cuuaaauccg auaagguccu caaacugcug     1440

ggauacucuc cccaggaaug ggagaucaac uacaaucagc ucgaaggcaa cagaacccaa     1500

aaggcccugu augaggcuua ccuuaaaauu guugaaaugg aggcccacga cgugaaggac     1560

auccugcaga uuaagagcac aaaagaugac uggucccucg augagagccc ucugucugcc     1620

uccgaaaucc gcgagagcgu gaaggcuauu uuccaggccc uggggaucug uaccaaaauc     1680

cuuuauuuug acccgcuccu gcccgucaag gauuucgaag aacaagacuc uuaccagcug     1740

uggcaucucc uguauuccua cgaaagcgau gacuccacgu cuggaaauga gacacuuuau     1800

cggauucugg agaaaaagua cgcguuuaag agagaacacg cucgcauccu cgccaacgug     1860

gcccugcagg augacuacgg cagccugucc accaaagcua uucggaagau cuauccuaau     1920

auuaaagaga accaguacuc uaccgccugc gaaauggcug gguauaagca uagcaaacuu     1980

ucccucacaa ccgaggaacu ggaggccaga gagcugaaga acaucauccc ccuccugaag     2040

aaaaaugcgc uucgcaaccc uguuguggaa aagauucuga aucaaaugau caacgucgug     2100

aaugcccuca uugagaaaaa cagcgaacgg gaugcugagg gaaagaucac aaaauacuuc     2160

cacuuugacg agauuagaau cgaacuggcc cgggagcuga agaaaaaugc ucagaagcgc     2220

uaugaaauga cgcagaacau caauaaggcc aaaucugagc accagaagau uuccgaaauc     2280

cuucaaaaag aguucggcau uaagaacccc accaaaagcg auaucauuag auaccggcuc     2340

uauaaggagc uggaacauaa uggcuacaag gagcuguaca caaacaccua uaucgcgccg     2400

gacguucucu uuucuaaaaa uaucgacauu gaacacaucg ugccuaaggc cagagucuuc     2460

gaugacuccu ucagcaacaa aacacuggcu uuuaaggauu ucaauauuaa aaagggggag     2520

ucuaccgccu uugacuacau cacgagccuu aagaacgaag agaaacugaa ccaguaucuc     2580

gcucgcgugg agaaugccua caagaccaaa aacauuuccc ccgccaagua uaaaaaucug     2640

cugaagaagg cuagcgaaau cggagauggc uucauuaacc gggaccuuag agauacacag     2700

uacaucgcga aaaaggccaa agagauccug uuucagguca ccaagaaugu gcucucuaca     2760

uccgggagca uuaccgaccg ccugagagaa gauugguccc ucguugacgu gaugaaagag     2820

cugaacaugc cuaaguauca aucucuugga cugacggaag ucgaggagcg gaaggauggc     2880

aauaaaguga caguuaucaa ggacuggacc aaacgcaacg aucauagaca ccaugcuaug     2940

gacgcccuca caguggccuu caccaagccc agcuacauuc aguaccugaa ucaccugaac     3000

gcucggaaag acgaaaauaa caagaauuau uccgucaucc uugccauuga gaaaaaggaa     3060

accaucaagg ugcccacaaa caaugggaaa aacaagcgcg uguuuaucga gccuauuccg     3120

aacuucagac agguugcgaa aaagcaccug gaggaaaucu uuauuucuca uaaagcuaag     3180

aauaaggucg ugacgaaaaa caccaauaag cccgccggca caaacaaaca acagaucacc     3240

cucacaccuc ggggacagcu gcacaaggag accauuuacg gcaaauauca guacuaugug     3300

aauaaggaag agaaggucgg ggugaaauuc gauaaggaaa cgaucgccaa aguuagcaac     3360

ccccucuacc gcgaggcucu gcuuaagaga cugcaagcca augacaacga uccuaaaaag     3420

gcguuuacag gaaagaaugc ucucuccaaa aaccccaucu aucuggacga ggccaagacc     3480

aaaacccugc cggaaaaggu gaaucuuaca uaccuggagg aagauuucuc uguccggaaa     3540

aagauugugc cuaaaaaccc caccagcaag gaguuuguuc cuaaucucaa guccaucgaa     3600

aaaguggucg acgagggcgu gcgcaacauu cugaucgaga gacuccgggu uuacaauaac     3660

gaucccgaaa aggccuucgu ggaccuggag aaaaauccua uuuggcuuaa caaggaaaaa     3720

gggaucgcua uuaagagagu cacaaucagc ggagugaaca augcccagcc ccugcauauc     3780

ggcaaggauc accuggggaa aacgacccuc aacaaggagg gcaaagaaau uccgguggac     3840

uaugucucua caggaaauaa ccaccauguu gcuaucuacc gcgauaagga gggcaaucug     3900

caggagcaga uuguguccuu cuuugaagcc gugguccgga cccaacaggg gcuuccuauc     3960

auugacaaaa cauauaagca ggaggaagga uggcaguucc uguuuaccau gaagcaaaac     4020

gagauguucg uguuucccaa ugaggcgacg ggcuucaacc ccgccgaaau cgaccuccug     4080

gauccuaaaa auaagaaaca gaucagcccg aaccucuuua gaguucagaa gauugcuacc     4140

aaagacuacu ucuuucgcca ccaucuggag acaaaugugg aaaccgauaa cguccuuaag     4200

aauacaaccu ggaagcggga ggggcugucu ggacugaaag acaucgugaa gguuagaauu     4260

aaccaccucg gcgauaucgu guccauuggg gaauauuga                            4299


<210>  4513
<211>  4302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4513
augaagaaaa uccugggccu cgaccugggg accacaagca uuggauacgc ccaggugaac       60

gaguccgaag auuucaagga cucuagcauc gagagaauug gcguccgggu uaauccccuu      120

uccguggaug aacaaacgaa cuuugagaaa gggaagccua ucucuauuaa ugcugaccgc      180

acccugaaaa gcggagccag acggacacuc gaucgcuauc aggacagacg guccaaccug      240

aucgaugcgc uugugaaguc ugucaucauu accgaagaca cagugcuggc ugaggauggc      300

aagaauacca cacacagcac cuucgcccuc cgcgccaaau ccgcuagcga gaagguugaa      360

aaagccgagu uugcuagaau ccugcuggcc auuaacaaga aacgggggua caagucuucc      420

cgcaagacga auaacaauga cgaugaagga caggcgaucg acggcauggc cauugcuaaa      480

aagcucuaug augagaaccu gaccccgggc cagcuugccu accaacugcu caaagagggg      540

aagaaauauc ugcccgacuu cuacagaagc gaccuucagg cugaauuuga uaagaucugg      600

acauuccaga aacaguuuua uccugagauu cugaccgacg aacuccaaaa ggagcuggcc      660

ggaaagggcc agcgggcgac aucugccauc uucuggaaaa aguacgauuu uaauaccgcu      720

gaaaacaaag ggacgcgcga ggagaagaaa cugaaggccu acgaauggag auccacaagc      780

guggacaaga aacuccagaa ggaggaaguc gcuuauguga ucaccgagau uaauaacaau      840

cugacaaacu cuuccggaua ccuuggcgcc aucagcgauc gguccaaaga acuguauuuc      900

aacaagcaga ccguugggca auaccucuuu aaacagauuc agaagaaucc ccauaccaag      960

cugaaaaacc aaguguucua ucgccaggac uacuuugaug aguucgagau cauuugggaa     1020

acacaggcca aguuccaccc ugagcuuacg gaagaacuga aaaauaagau cagagacauc     1080

auuaucuuuu aucagcggaa acucaagucu caaaagggac ugauuagcuu cugcgaauuu     1140

gagagaaaag aggucgaaau cgagaagggc ggccgccgga ugaaaaagac cauugggcug     1200

aaaguggcuc ccaaguccag cccucucuuc caggaauuua aaaucuggca agugcugaac     1260

aauguccuua ucagaaagaa gggaucuaaa aagcgccggg uuaaagagcc ggaucagcug     1320

aaccucgugg agcaggaaca ggaggugaug uucuuugaca ugcagacaaa gcagacccug     1380

uucgaugaac uuaaugucaa aggcaaccug aaggcgaaga gaauuaucga gcucauuggg     1440

caagacgcca augacuggga aaucaacuac uccgagauug agggaaaucg cacaaacaaa     1500

gcucuguacg augccuaucu gaagauccuc gaccuggaag gcuacgauac caaagcccuu     1560

cuggacguga agacgagcaa agaugagguu gaacucgacg auauuaauau cucugggccc     1620

gaggugaagg accuggucaa gcggaucuuu gaaagccugg agauugauac acagauccuu     1680

gaguucgacg cugaacucga uggagaggug uuugaaaaac aguccucuua ucugcugugg     1740

caucuccugu acagcuauga gggcgacgau uccccuagcg gcaacgaaaa acuuuacaaa     1800

cugcucaagc agaaauucgg guuuaagaag gagcacgccc aaauucuggc gucuaucacc     1860

cuguccgacg acuauggaag ccuuucugcu aaagccauga gaaagauuua ccccuacauc     1920

cgcgagcaga ccuucgauaa agccgcuucc cucgccggcu aucggcauag cgcuuccucu     1980

cugacaaagg aagagaucgc caauagaccu cugaaagaca agcucgaacu gauuaaaaag     2040

aacagccuuc gcaaccccgu uguggagaag auccugaauc aggucgugaa cauugucaau     2100

ucccucaucg aggcgaacuc ugaaaaagau agcgacggga auauuaucuc cuacuucaag     2160

uuugaugaga uccggauuga acuggccaga gaccugaaaa agaacgcuaa agagcgggcc     2220

gaaaugaccc uuaauaucaa cgcugccaag auugugcacg agaagaucuu caaacuccug     2280

caagcggagu uuggaguuaa gaauccgaca cgcaacgaua uuaucagaua uaaacuguac     2340

gaagaacuca agaauaacgg cuauaaagac cuguacacga auaucuauau uccucgggaa     2400

auccuuuuca gcaagcagau ugauaucgag cacauuaucc cccagucuaa gauguuugac     2460

gauuccuuca gcaacaaaac cauuguguuu agaaaggaca aucuggauaa agggaacucu     2520

acagccuucg acuacaucga cucccgcuuu ggcaaggaua aacucaccga cuucgaacag     2580

cggaucgagu uucuguucga gcaaggaaag aagaacaaag aagagggcau uagcaaggcu     2640

aaauaccaga agcugcuuaa aagagaagcc gauaucgggg acggauuuau ugagcgcgau     2700

cucagagaca cacaguauau cgcuaagaag gcccggaauc ugcuguacga aauuugucgc     2760

aucgucaccc cuacgaccgg cucuaucaca gauagacucc gggaggacug ggaucuggug     2820

aacauuaugc aggagcuuaa ucuggacaaa uucaagaaac ucgggcugac cgaaaugguu     2880

gagaagaaag auggcagcuu caaggaacgc aucguggacu gguccaaaag aaacgaccau     2940

cggcaccaug ccauggaugc ucugacaauu gcguuuacca agcacagcca cauccaauau     3000

cuuaauuacc ugaacgcccg caagaaugag aaccauaaac agcaccauaa uauuaucgga     3060

aucgagagaa aggaaacgga guucgucauu gacgaucucg ggaacaaaaa gcggaaauuu     3120

caucugcccc uccccaauuu ccgcgaagag gcuaagaagc accuggaaaa cgugcuugug     3180

ucuuuuaaag ccaagaauaa aguugucaca aagaacaaaa auaagaccgc cuccagcaag     3240

ggcgagaaaa ucaaggugga gcugacaccu agaggacagc uccacaaaga aaccguguau     3300

ggcaaguaca aauucuaugu cuccaaggag gaacggauug ggggaaaguu ugaccagaga     3360

accaucgagc gcguggcuaa cucuacacac aaacggcugc ugcuucaaag acugcucgaa     3420

aacgauaaug acgccaagaa agcguucacg ggcaagaacg cucuggccaa aaauccgauu     3480

uaccucgaug acaccaagac acugacccuu cccgagaucg uuaaacugac auggcucgag     3540

gaagauuaca gcauucgcaa ggacaucacc ccugagaacu uuaaggauau caaagugauu     3600

gacaagaucc uggaugaagg ggucaaacgg auucugaagg agagacuugc caaacauaau     3660

aacaauccca aggaggcuuu cuccgaccug gagaagaacc cuaucuggcu caaugagaaa     3720

aagucuauua gcaucaaaaa ggugaaaauc uccggaauua acaaugccga agcgcugcac     3780

uauaagaaga accaucucgg caucgagauu cuggacgaug agaaaaagcg cauccccguu     3840

gacuuugugu cuacggggaa uaaccaccac gucgcuauuu accgggauga gaaaggcaau     3900

cuucaggagg acgugguuag cuucuucgaa gccguggcca gagucaacca gaaucugccg     3960

aucaucgaua agacacugaa ggcuuaucuc ggauggaccu uucuguucac caugaagcaa     4020

aacgaguacu uuauuuuccc uaacaagcag acaggcuuua aucccaccga aaucgaccuu     4080

cuggauucca ugaacaauaa acucauuagc ccuaaccugu uccgcgugca gaaguuuaca     4140

gugaaagacu auuucuuucg gcaucaccuc gagacgaaug ucgaagauga cucuaagcug     4200

aaaaacacca cauggaagag agaggggcuu ggaggcaucg ggaauauugu uaaagugcgg     4260

aucaaccaua uuggacagau cgugaagauc ggcgaguacu ga                        4302


<210>  4514
<211>  4305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4514
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugggc cuacgugaaa       60

caggcucaaa acgauuccga gaagucugaa aucgccgagc uuggcgucag aguuaauccc      120

cugacagugg acgaaaagac gaacuucgag aaggggaaac cuauuagcac caaugcggau      180

cggacacuca agcgcggagc uagacggaac cugcagcgcu auaaacuuag acggcaggaa      240

cugaucgagc uccugaaaga cuaccaggug auuuccgaua agaccauccu ggccgaggac      300

ggcaaagaaa caaccuuuga gacacuccgc cugagaguca agucugccaa agaacaagug      360

agccuugagg agcuggcucg gauccuccug augauuaaua agaagcgcgg guauaaaucc      420

ucuagaaagg ccaaaaacga agaugaggga cagcuuaucg acggcaugga aauugcucgg      480

cgccuguacg augagaaucu caccacgggc cagcugagcc ugcagcuccu gaaggaaggg      540

aaaaaguccc uuccggacua uuacagaagc gaucugcaac acgagcucga caaaguuugg      600

aacuuccaga agcaguuuca ucccgauauc cugaccgacg aguucuauac aucuauucag      660

ggaaagggcc aaaaagccac ccgggaaucc uuuagcucua aguacgggau cgacacagcg      720

gagaauaaag gaucccgcga agagaagaaa cuucaggccu accaguggag agcugaugcc      780

acccacaagg aacugacgaa ggaggagcuc gcuuucguga uuacagaaau caacaauaac      840

aucaauaaca gcucuggcua ucugggggcc auuuccgacc ggagcaaaga gcugaucuuu      900

gcgaacgaaa ccgucggaga guaccucugg aagcaaauuc agaaugaucc ucauacaucc      960

cugaaaaacc agguguucua uagacaggac uacuuugaug aauuugagcg caucugggag     1020

acccaaucug gcuuuuaucc ccagauucuu accgaaagcc ugaagaauga gcuccgggac     1080

auccugaucu ucuaccagag aaaacuuaag ucccagaagg cccugguuag cauuugcgaa     1140

uuugagucua aagagauccc ucucaaggau aaacaaggca accccauuuc caagaauggg     1200

aucccuguga caaaacuggu cggaccgaag guggcuccca agagcucucc ucuguuccag     1260

gaguucaaaa uuuggcagaa ccucaauaac gugcuggucc gccccaaggg cagccggaaa     1320

agaaagguua cgaccaauca gcaauccaca cuucuggaag ggcaggaacc uguguuugag     1380

cucaaccugg aacagaaaga gcugcuuuuc gaggaacuca aucugaaggg aaaccugaaa     1440

accgacuauu gucucgagcu gaucggcuac ucugccaagg aaugggagau gaauuacccc     1500

acacuugaag ggaacaagac caauaaagcu cuguaugagg ccuaccucaa gaucauugau     1560

agcgagggau augacgccaa aaagcugcug aaagugaaga cggauaagga cgacaucaac     1620

cuugaugaca uuaaagucuc cgcuagcgaa aucaagacaa ugauuucuga uaucuuuaac     1680

gagcucggca uuaauaccaa aauccuggac uucgaugcgg aacuggagcg caagcaguuu     1740

gaagcccagg cuuccuaccg gcucuggcau cugcuuuaua gcuacgaggg cgacgauucu     1800

aaauccggga acgagcugcu cuauagacug cuggaagaca aguucggauu uaagcgcgag     1860

cacagcaaaa cccuugccaa ugugucccuc cugucugauu acggcaaccu gagcacaaag     1920

gccauccgga ccauuuaccc guauaucaaa gaaaauaagu ucuccacagc uugcgagcuc     1980

gccggguaca gacacucuaa acugagcauu uccaaggagg aaaacgagaa ucgcacgcuu     2040

aaggacaccc uggauguucu caaaaagaac agccugcggc aaccuguggu cgagaaaauc     2100

cugaaucaga ugauuaaccu uaucaaugaa cucaucgaaa agcacucuga gaaagacgag     2160

aagggaaaca uugugaaaua uuuuaaguuc gaugaaaucc auauugagcu ggcgagagaa     2220

cugaagaaaa augcugagga acggcgcgag cucgacucca agguuagaga gggcaaagac     2280

cggaacgaaa agaucauuac aauccugaaa agcgaguuug ggcuucccua cccuaccaag     2340

aaugauauca caagauaucg ccuguacgag gaacucaagg acaacggcua uaaagaucug     2400

uacaccaaca cguacauucc ccgggaaaag cuguucucua aagagaucga cauugagcac     2460

aucauuccca aguccagacu uuuuaaugau agcuucucua acaaaaccau cuguuaucgc     2520

caagacaauc ugaacaaggg agaaagaaca gccuacgauu auaucgaggg ccucgggaag     2580

acccagcugg acgaauaccu caaucgggug gagaaccugu auacaaauaa auucauuacc     2640

aaggccaaau accgcaaccu ucugaagaaa gagagcgaaa ucggagaugg cuuuauugag     2700

agagaccucc gggaaacgca guauaucgcu aagaaggcca aagagaugcu gcugcagauu     2760

acacgccaug ucguguccac cacagggagc aucaccgaua gacuucggga cgauugggaa     2820

cuggucaaug ugaugcgcga gcucaaccug ccuaaguaca aaguucucgg acugaccgag     2880

auucaagaaa gaaagaaugg ccagacagug gaggucauca cggacuggac caaacggaac     2940

gaccaccgcc accaugcuau ggaugcccuu acaguggcgu ucaccaagca cucucauauc     3000

caguaccuga auuuucucaa cgccagaaag gacgaaaaac acaaggagua uuccaauauu     3060

auggggaucc agcaacugga aacagagaaa accacggaua aggacggcaa agaucggaga     3120

guuuucaaga caccgauucc caacuuucgc cagguggcua aagagcaucu ggaaagcauc     3180

cuuauuuccc acaagaccaa gaacaaaguc gugacccggg ccacaaauaa gaucgcugga     3240

ucuaaaaagc agcaggagac ccugagcccu agaggccaac uccacaaaga aacaguguac     3300

gggaaguauc gccgguacgu ucagaaggag gagaaaaucg gauccaaguu cgaccugcag     3360

acgauugaaa gagucgccaa cccccucuau aaacgccugc uuaccaagcg gcuggaggaa     3420

aauggcgggg auccuaaaaa ggcguuuaca ggaaagaacg cccuggcuaa aucucccauc     3480

uaccucgacg agacccaaac acagcagcug ccggaaaagg ugaaacuugu guggcuggag     3540

accgauuuca gcauuagaaa ggacaucacg ccugauaauu uuaaagacga gaaguccauu     3600

gacaaggucc ucgaugaagg caucaaacgc auccugaagg accggcucaa aucuuucgau     3660

ggggacgcca agagagcuuu uaccgaucug gagcagaacc ccauuuggcu uaauaaagaa     3720

aagggcaucg ccauuaaacg ggugacaauc agcggaguuu ccaacgccga gccucugcau     3780

accaagaagg accaccuggg caaugaaauu uucgaugaca cagggaaacc caucccugug     3840

gauuauguca gcaccggaaa caaucaucac auugcuaucu accgcgacga gaacggcaau     3900

cuccaagaga aggcgguguc uuucuucgaa gccguuacaa gagcuaacca ggggcugccc     3960

accguggaua aaacguacaa ucagcaccuu ggauggcagu uccuguuuac aaugaagcaa     4020

aacgagaugu ucgucuuucc gaauaaagaa accggcuucg acccucagga gaucgaucuc     4080

cuggacccca agaacaagaa acucauuucc cccaaccugu uucgggugca gaagaucagc     4140

accaaaaauu auuucuuucg ccaucaccuu gaaacaucug uugagaccca uaacgcccug     4200

aaugggguga cguacaaguc ccagcugggc cucaacggaa uuacagacau cauuaaaguc     4260

agaaucaauc accugggcga uaucgugcau auuggggagu auuga                     4305


<210>  4515
<211>  4308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4515
augcugggcu gcgagagaaa gcggaaacgc agagaaaagg agaaaauggc caagcccaaa       60

gacuacacca ucgggcucga uauuggaaca aacagcgugg gcugggucgu uacggacgau      120

cagaauaaca uccugcggau uaaggggaag aaagcuaucg gagcccgccu uuucaccgaa      180

ggcaaggugg cggcugagag acgguccuuu cgcacaacca gacggcgccu gucuagacgg      240

cgcuggagaa uuaaaaugcu cgaagaacug uucgacgagg aaaucgccaa gguggauccu      300

agcuuuuucg cccggcuuca cgaguccugg aucucuccga aagacaagcg caagagauau      360

agcgcuauug ucuuucccuc cccugaagag gauaaaaagu uccaugaguc uuaccccaca      420

aucuaucauc ugcgggacaa acucaugaag gaugaccaaa aacacgauau uagagaaauc      480

uacauugccg ugcaccagau gaucaaggcu cgcgggaauu uucugcauga cgagagcguu      540

gaaaccuauc gguccggaau gagcucucug ggcggcagau ccgagcgcaa cauucucagc      600

gugcagacac uggaagaacu uaaugaucug uucgccgaga acgaagggac cgaggaaguc      660

gagcucaaug uggcgucugc cgaacagauc aacgacaucc ugacgggagg ccaccuuaau      720

gcugacuccc aaaaagagau uagcaaccug cuccugccuu cuuccuuucc cagcuucgau      780

gacaaggcca aggagaaaca gguuaagaaa cugaucaaua acguggcuac caauauuucc      840

aaggccuggc ucggguacaa agcggauuuu ucuacaaucc ugaaccuugc caaggucgac      900

aaggaucaga aaaagauuuu cgcuuuugcc cugcagggag gcgacgaaga ggauaaagug      960

caagaacucg agagccugcu ugaacagucc cagaccgaca ucguggaucg gcugaucgag     1020

auuagacacg cuaucguccu cagcgagauu guuccugugg ggaugacacu gucugaagcc     1080

augaucgaca aguaugauca gcauaaagag gaccugauua cccucaaggc cgugauccgc     1140

aauacgaagg acaaaaagaa agcugcgaag cugcaagcca ucuacgaucu uuacgucaaa     1200

aaccggcacg cugaccuggc caaggccaug aagcucacag gaauuaaaaa gagauccgaa     1260

cugcuugauc cggaggagcu gaaaaagggc aucagcucuc uccuggacga uagccccgaa     1320

gcuguggaga uuaaacagcg ccuggaagag aagaccuucc ucccucugca gcgguccaac     1380

aauaacggcg uuauccccaa ucaacuucau cagguggaac uggacgagau uaucaaaaag     1440

cagucuaagu auuacccuuu ccucgccgag aaaaaccccg augaaagcga ggaagcgcag     1500

aagaaagcuc cgacaaagcu ggacgcccug cuuaccuuua gagucccuua uuacgugggg     1560

cccaugauua ccaaagagga acaagaggcc cagaauggac acuccuucgc uuggaugguu     1620

cgccgggauc cucaggacca cgaggccauc acacccugga acuuugaaaa gaagguggau     1680

aaaauggcua gcgccacgca guucaucaag agaaugacca caaaagacac cuaucuccug     1740

ggcgaggaug uccugcccgc gucuucccuc aaguaccaac uguuuacagu gcuuaaugaa     1800

cugaacaauc uccgggucaa cgggaaaaag cugaccagcg acgagaagga acaggugauu     1860

gagggacugu ucaaaaagca gaaaacgguu aaggccgaca aauuugugaa guauuggcag     1920

gcuaagcaua ucggcgccga uauuaaaguc aaggggcuuu cugacccuuc caaauucaau     1980

agcacaaugu ccaccuacau cgauuuuaag aaaauuuucg gagaccaacu caacgaugug     2040

aaucgccaga acgacuuuga gaagaucauc gaauacucua ccauuuucga ggauagaaaa     2100

auccuggaag acaagcugcg gguugauuuu gcuuggcuca gcgaggagca gauuaagcag     2160

cuguguagac uucgccugca aggcuggggc cggcucuccg acaaacugcu gacaagacuu     2220

accgaugccg acgggcagaa ugugcucgaa cgccugugga acucuaacga uaauuucguc     2280

cagaucguga gcgacccgaa cauuaaggcg aaaaucgagc aagaaaauca gaagcugcuc     2340

agagacuccc agagcgauaa cgccguggag ucuauccugg acgaugcuua uacaagcccc     2400

cagaauaaaa aggccauucg gcaaguuaug cgcgucgugc acgacaucgu gaaggcuugc     2460

gauggaaacg ucccugccaa auucgccauu gaauuugcua gaucccagga ggacgauccc     2520

aagcggacga ccgcgcgcgu gaaucagaug cagaaaaucu acgaccaaau uucugaugag     2580

guuguguccc agggcgucaa ggaacagcuu agcgggauga aaucucugaa ggacagauau     2640

uaccucuauu ucaugcaggg aggccgggau gccuacacag gggcuaagcu gaacaucgac     2700

cgccugagcg acuaugaugu ggaccauauc augccucaau ccuuuguuaa agaugacagc     2760

cuugauaaua gagugcucgu cgcccgggcc gugaacaauc agaagucuga caaaguuccc     2820

gcucugcugu ucggcaacaa gguggucgcc gaucucggaa uuaccgugcg cgagaugugg     2880

gacaaauggc agaagcuggg caugaucucc aagagaaaac uuagcaaucu gcucacagau     2940

uccgacgcgc ugaccgaaua ccgggcucaa ggguuuauua gacgccagcu gguggagacg     3000

ucucagguca ucaaacuuac cgccacaauu cugcagagcg aguucccgga uuccaaaauc     3060

auugagguuc cugccaagua caacucuauc gugcggaaac aauuugaccu cuauaagagc     3120

agagagguga augauuucca ccaugcuauc gacgccuacc uguccaccau ugucggaaac     3180

uaucucuacc agguguaucc caaccugcgc cggauguuug uuuacggcga guucaagaaa     3240

uuuucuagca augcggagga auccgcucac gacguggcca gacgcgucaa gagcaugaac     3300

uuccuugaug accugcuccg ggggacacac ggagauaaua ucuauuguag aucuaccggc     3360

gagauugugu uuaaccgcaa ugacaucauu uccaaacuga agcaggccua cagcuucaaa     3420

cagaugcugg uuacgcaaga aguguucaca aagaaaucug cucuuuuuaa ccagaccguc     3480

uacccuuccc ccaagcggga ugggagcaag agaucuggcc ugaucccucg gaaacgcgga     3540

auggaccccg agaucuaugg cggguacgcc cauaaucagg auuccuauuu cgugcucgcu     3600

aagauugaga aaguuaaggg auacacacug cagaucgugg gcgucccgau uagaagccuc     3660

gaaaucauua acaaagccga ggacuuuucc accgcgcugc uugauaucau caagagcaag     3720

gugauguaua augaaaagac cgggcaaccu aagcggggaa uuaaaaacgu ggagauccug     3780

gucaauaagc uccccuaccg ccagcccauu cuggagaaag acguuucuua uaugcugggc     3840

agcuccgccu acaaguacaa caucaagcag auuacacuuu cuagacagag caugaaauau     3900

auccuggauu acauugacga uccuaauuuc acgaagcacg aaaugaccau gaucgacgag     3960

caaaacgaac gggagugccu ccuguccgug uaugacgaaa uccucgagaa aguggauaag     4020

uaccugccgc uuuuugacgg gaauaaauuc agagagaagc ugcauucugc ucgcaaggaa     4080

uuugucagcc uguccgugga ugacgaggcc ucugcuauug agaaaauccu ccggggccug     4140

cacgccaaca gcgaaaugcc cucccuugaa aaccugaaga uuaaaccugc ccucggauuc     4200

uuucagauga agaauggcau cacacugagc aacgaugcug uucuccugua ucagucuccc     4260

accgggcuuu ucgagagacg cgugaaaguc uccgaccugc ugaaguga                  4308


<210>  4516
<211>  4308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4516
augaagaaca uccugggccu cgaccugggg accacaagca uuggauucgc ccacaucgug       60

gaggaugaaa auaaagagaa guccgaaauu aaagagcuug gcgucagaau cguuccccug      120

acgaccgacg aacagucuga uuuugagaag gggaaaagca uuacaaccaa cgcuaaucgg      180

acacucaagc auggagcccg ccugaaccuu gacagauacc aacagcggcg caaguaucug      240

aucgaucucc ugcagaaagc gaaucugauc accuccucua gcauucucgc ugagaacggc      300

aagaauacaa cccacuccac guggcagcug agagccaaag ccgugaccga acggaucgag      360

aaggaagagu ucgcucgcgu gcuucuggcc auuaacaaaa agagagggua caagucuagc      420

cggaaagcua agacagagga cgaaggacaa gccaucgaug gcauggcgau ugccaaacgc      480

cucuaugacg agaaucugac cacaggccag cuuucccugc agcuccugca gcaaaacaag      540

aaacugcucc cugauuuuua cagaagcgac cugcagaagg aauuugaucu ugucuggaau      600

uuucagaaac aguucuaucc ggacauccug accgauucuu uuuacaagga gcuccaaggg      660

aagggaaaag acgcuacguc caaggccuuc agcaaacggu aucauuuuga cacagcugaa      720

aacaagggcu cuaaagaguc cgugcgccug caggccuacc aguggagagu ugaggcgauu      780

agcaagcaac uuucuaagga agagguggcc uacguccuga ccgaaaucaa uaacaaucuc      840

aacaacgcuu ccggguaucu gggagccauc agcgaucggu ccaaagagcu guacuucaau      900

cgccagacag ugggccagua ucucuacgcu cagcugcaag aaaacagaca caauucucuu      960

aagaacaaag uuuucuaucg gcaggacuac cuggaugagu ucgagagaau uugggaaacc     1020

caggccagcu uucauaagga gcucaccgag gaacugaaaa agcagauccg cgacgugguc     1080

auuuucuauc aacggaagcc caaaucccag aaggggcuua ucagcuucug cgaauuugag     1140

ucuaaagaga uugaaaucga gaaggaugga aaaacaauca cgaagaccau uggcgccaga     1200

guggugccua aguccagccc ccuguuccag gaauuuaaaa ucuggcagau ucucaauaac     1260

gucaucugua agcgcaaagg cauucggaag aaaaagguuu cugcuaaaac aacccaacug     1320

gaccugcuca augagagcuc ccagacaauc uucucucugg auauggagug caagcagcuu     1380

cuguuugagg aacucaaccu gaagggggac cugaaaagcg auaaggugcu uaaacuccug     1440

ggauacuccc cucaggaaug ggagaucaau uacaaccaac uggaaggcaa uagaacccag     1500

aaggcgcucu augaggccua ccugaaaauu guggagaugg aagcucacga cgucaaggau     1560

auccuucaga uuaagagcgc caaagacgac uggucucugg augagagccc ccucuccgcc     1620

ucugaaaucc gcgaguccgu gaaggcuauu uuccaagccc uggggaucug uacgaaaauu     1680

cuguauuuug acccucuucu cccgguuaag gaguucgaaa aacaggauag cuaccagcug     1740

uggcaucugc ucuauuccua cgagucugac gauagcacau ccggaaacga aacccuguau     1800

cggauccuug agaagaagua cgcguuuaaa agagaacacg ugcgcauccu ggcuaauguc     1860

gcccuccagg acgauuacgg cucucugagc accaaggcca uucggaaaau cuaucccaac     1920

auuaaggaga aucaauacuc caucgcuugc gaaauggccg gguauaaaca cagcaagcug     1980

ucucuuacaa ccgaggagcu cgaagcuaga gagcugaaga acauuauccc ucugcucaaa     2040

aagaacgccc ugcgcaaucc cgugguugaa aagauccuua accagaugau uaaugugguc     2100

aacgcgcuga ucgagaaaaa uuccgagcgg gacgccgaag gaaaaauuac aaaguacuuc     2160

cauuuugaug agaucagaau ugaacucgcu cgggagcuga aaaagaacgc ccagaagcgc     2220

uaugaaauga cgcagaauau caacaaagcu aagcuggagc accagaaaau cagcgagauu     2280

cuucaaaagc aguucggcau caaaaauccu accaagucug acauuaucag auaccggcuc     2340

uaucaggaac uggagcacaa cggcuacaag gaacuguaca caaaugccua uauugcgccc     2400

gaugugcucu uuuccaaaaa caucgacauc gagcauauug ucccgaaggc cagaguguuc     2460

gaugacagcu ucucuaauaa aacccuggcu uuucgcgagu ucaacaucaa gaaaggggaa     2520

agcacagccu uugacuacau uaccucccuu aauagcgagg aggaacugaa ccaauaucuc     2580

acgcggguug aaaacgcuua caagaccaag ucuaucuccc cuacaaaaua uaagaaucug     2640

cugaaaaagg ccagcgagau uggagaugac uucaucaaca gagaucuucg cgacacccag     2700

uacauugcca aaaaggcuaa ggaaauccug uuucagguga ccaaaaaugu ccucuccaca     2760

ucuggcagca ucacggauag acugcgggag gacugggggc ucguggaugu uaugaaggag     2820

cugaacaugc ccaaauauca aucccuugga cugacagaag uggaggaacg caaggacggc     2880

aauaaaguca ccgugauuaa gaacuggaca aagagaaaug aucaccggca ucacgcgaug     2940

gacgcccuca ccguggcuuu caccaaaccu ucuuacaucc aguaccugaa ccaucugaau     3000

gcccgcaagg augagaacaa uaaaaacuau agcguuauuc uugccaucga ggaaaaggag     3060

acaauuaaag ucagaacgaa uaacgggaag aauaaacggg uguuuaucga acccaucccc     3120

aacuuccgcc agguggcuaa gaagcaccug gaggaaauuu uuaucuccca caaaaccaag     3180

aacaaagucg ugacaaagaa uguuaacaaa ccugccggca ccgacaagca gcaaauuaca     3240

cucaccccga gaggacagcu gcauaaggag acgaucuacg gcaaauauca guacuaugug     3300

aauaaggagg agaaaauugg ggucaaguuc gauaaagaaa caaucgcgaa ggugucuaac     3360

ccccucuacc gggaagcucu gcuuaagcgc cugcaggcca augacaacga cccuaaaaag     3420

gccuuuaccg ggaaaaaugc ucucagcaag aaacccaucu aucuggauga guccaagacc     3480

aagacacugc cugaaaaagu uaaccuuacc uaccuggagg aggacuucag cauuagaaag     3540

gauaucucuc ccgacaauuu uaaagaucuc aaguccauug aaaaagugau cgaccaaggc     3600

gucaagcgga uucugaucaa aagacuccag gccuacgaua acgacccgaa gaaggcguuc     3660

guggaucugg agaaaaaucc uauuuggcuu aacaaggaaa aagggaucgc uaucaagcgc     3720

guuacaauua gcggagugaa uaacgcccag ccccugcaca ucggcaaaga ccaucugggg     3780

aagacgaccc ucaauaagga gggcaaagaa auuccugucg auuauguguc uacaggaaac     3840

aaucaccacg uggccaucua ccgggacaag gagggcaacc ugcaggagca aauugucucc     3900

uucuuugacg cuguugugag agcccagcag gggcuuccca ucaucgauaa aaccuauaag     3960

caggcugaag gauggcaauu cauguuuaca augaaacaga acgagauguu cguguuuccu     4020

aaugaaacca cgggcuucaa ccccgccgag auugaccugc ucgauccgaa gaauaagaaa     4080

cugaucagcc cuaaccucuu ucgcguccag accauuucug ugguuaagua cggaaauucc     4140

gugguccggg acuucaaauu uagacaucac cuggagacaa cccuucaaga ugacaagauc     4200

cugcagggaa uuacauucca gcagaucaaa agccuggaac cccuccgcaa caucgugaag     4260

guucggauua aucaucuggg cgauaucgug uccauugggg aguauuga                  4308


<210>  4517
<211>  4308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4517
augaagaaca uccugggccu cgaccugggg accacaagca uuggauucgc ccacaucgug       60

gaggaugaaa auaaagagaa guccgaaauu aaagagcuug gcgucagaau cguuucucug      120

gcuacggacg aacagagcga uuuugagaag gggaaaucca uuucuaccaa cgccaaucgg      180

acacucaagc auggagcgcg ccugaaccuu gacagauacc aacagcggcg caaguuccug      240

aucgaucucc ugcagaaagc uaaucugauc accagcgccu ccauucucgc cgagaacggc      300

aagaauacaa cccacucuac auggcagcug agagcuaaag ccgugaccga acggaucgag      360

aaggaagagu uugcucgcgu gcuucuggcc auuaacaaaa agagagggua uaagaauagc      420

cggaaaacga agaccgagga cgaaggacaa gcgaucgaug gcauggccau ugcuaaaugc      480

cucuacgacg agaaccugac acccggccag cuuucccugc agcuccugca gcaaaauaag      540

aaacugcucc cugauuucua ucgcagcgac cugcagaagg aauuugaucu ugucuggaac      600

cugcagaaac aguucuaccc ggacauccuc accgauucuu uuuauaagga gcugcaaggg      660

aagggaaaag acgccacauc caaggcuuuc agcaaaagau accauuuuga caccgccgaa      720

aauaagggcu cuaaagaguc cgugcggcuu caggcguauc aguggcgcgc cgaggcuauu      780

agcaagcaac ugucuaagga agagguugcc uacgugcuca cggaaaucaa caauaaccug      840

aacaauucca gcggguaccu gggagcuauc uccgauagau cuaaagagcu cuauuucaac      900

cggcagacag ucggccagua ccuguaugcc cagcuucaag aaaaucgcca caacagccug      960

aagaauaaag uguuuuacag acaggacuau cucgaugagu ucgagcggau uugggaaacc     1020

caggccuccu uucauaagga gcugacagac gaacuuaaaa agcagaucag agauguugug     1080

auuuucuacc aacgcaagcu gaaaagccag aaggggcucg ucucuuucug ugaguuugaa     1140

uccaaagaga ucgagauuga aaaggacgga aaagcuauca ccaagagcau cggcgcgcgg     1200

guggugccca agucuagccc ucuguuccag gaguuuaaaa uuuggcagau ccugaacaau     1260

gucacaugca agagaaaagg cauucgcaag aaaaaguccu cugccaaaag cacagaacuc     1320

gaucuguuca acgagcccuc ccaaagcauc uuuucucuug acauggagug uaagcagcuc     1380

cuguucgaug aacugaaucu gaagggggac cuuaaauccg auaagguucu caaacugcug     1440

ggauauagcc cucaggagug ggaaauuaac uacaaucagc ucgagggcaa ccggacgcaa     1500

aagucucugu acgaggcuua ucuuaaaauc aucgaaauug agggguacga agugaaggac     1560

auccugcaga uuaaguccac caaagacgau uggagccucg acgagucucc ccuguccgcc     1620

agcgaaauca gagagcgcgu gaaggucauu uuucagacac ugggaaucug caccaaaauu     1680

cuuuauuucg auccucuccu gccggugaag gaauuugagc aacaguacuc cuaucagcug     1740

uggcaccucc uguacucuua ugagagcgac gauuccacaa gcggcaauga aacccuuuac     1800

cggauccugg agaaaaagua cgccuucaag agagaacacg cuacgauccu cgccaacguu     1860

gcgcugcagg acgauuaugg gucucugucc acaaaagcua uucgcaagau cuaccccuau     1920

auuaaagaga aucaauacag caccgccugu gaacuugccg gauauaagca uucuaaacuc     1980

ucccugacca cagaggagcu ggaagcucgg gagcucaaga acaucauucc ucugcuuaag     2040

aaaaacgccc ugagaaaucc cguggucgaa aagauccuca accagaugau caaugugguu     2100

aacgcucuga uugagaaaaa uagcgagcgc gacgccgaag gcaagaucac caaauacuuu     2160

cacuucgaug agauucggau cgaacuggcg agagagcuua agaaaaacgc ccaggaacgg     2220

uaugagauga cacagaauau uaacaaggcu aagcucgagc aucagaaaau cucugaaaag     2280

cugcaaaaac aguuuggcau caagaauccu acgaaaagcg acauuauccg cuacagacug     2340

uaccaggagc ucgaacacaa cggguauaag gagcuguaca ccaauacaua uauugccccc     2400

gaugugcuuu ucuccaagaa caucgacauu gaacacaucg ucccgaaagc ucggguguuc     2460

gaugacagcu uuucuaauaa gacccuggcc uucaaagagu uuaacaucaa gaaaggagag     2520

uccacagcgu ucgacuacau uaccagccuc aauugcgaag gcgagcugaa ccaauaucug     2580

acgagagucg aaaacgccua caagaccaag uccaucucuc uuacaaaaua uaagaaucug     2640

cucaaaaagg cuagcgagau uggggaugga uuuaucaacc gcgaccugcg ggauacccag     2700

uacauugcca aaaaggcuaa ggaaauccuc uuccagguga ccaaaaaugu ucuguccacc     2760

ucuggcagca uuacggacag acuucgcgag gauugggggc ugguggacgu caugaaggag     2820

cucaacaugc cuaaauacca aucccugggc cugacagaag uggaggaaag aaaggaugga     2880

aauaaaguua ccgugaucaa ggacuggaca aagcggaacg aucaucgcca ccaugccaug     2940

gacgcccuua ccgucgcuuu uaccaaaccc ucuuauaucc aguaccugaa ucaccucaac     3000

gcgagaaagg augagaauaa caaaaauuau agcgugauuc uggccaucga gaagaaagaa     3060

acaauuaagg ugccuaucaa caauggcaaa aacaagcggg uuuucauuga gcccauccuc     3120

aauuuucgcc aggucgcuaa gaaacaucug gaagagaucu ucauuuccca caaggccaaa     3180

aacaaggugg ugacgaaaaa caucaauaag cccgccggga ccgacaagca gcaaauuaca     3240

cuuaccccua gaggacagcu gcacaaagaa acaaucuacg gcaaguauca guacuauguc     3300

aacaaagagg agaagguggg gguuaaauuu gaugaacgga ccauuacgaa ggugagcaau     3360

ccgcucuacc gcgaggcucu gcugaagaga cuucaggcca acgacaauga ccccaaaaag     3420

gcguucgcug gcaaaaacgc ccugucuaag aauccuaucu accucgauga agccaaaaca     3480

aagacccugc ccgagaaggu caaccucacc uaucuggaag aggacuuuuc cauccggaaa     3540

gauauuagcc cugacaauuu caaggaucuu aaaucuaucg agaaggugau ugaccaagga     3600

guuaaacgca uccugauuaa gagacugcag gcuuacgaua acgaccccaa aaaggccuuc     3660

guggaucucg aaaagaaucc gaucuggcug aacaaagaga aggggauugc gaucaaacgg     3720

gucacaaucu ccggcgugag caaugcucag ccucuucaua uuggaaagga ccaccugggc     3780

aaaaccacac ucaacaagga agggaaggag guucccgugg auuaugucuc uacgggaaau     3840

aaccaucacg uggccaucua cagagacaaa gaaggcaauc ugcaggagca aauugugucc     3900

uuuuucgagg ccgucguucg cgcucagcag gggcucccua ucauugacaa gaacuauaaa     3960

caggccgaag gauggcaauu uauguucacc augaagcaga acgagauguu uguguucccc     4020

aaugaagcua caggcuuuaa cccugccgag aucgaucugc uugaccccaa aaauaagaag     4080

cugaucagcc cgaaccuguu ccgggugcag accauuucca ucgucaaaua cgggaauagc     4140

gugguuagag auuuuaaguu ccgccaccau cucgagacaa cccugcaaga cgauaaaauu     4200

cuucagggca ucacguuuca gcagauuaag ucucuggaac cucuccggaa caucgugaaa     4260

gucagaauca aucaccuggg agacauugug aacaucggcg aguauuga                  4308


<210>  4518
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4518
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acaaagauga gcgguccucu aucgucaagc ugggcguucg cgugaauccc      120

cucacagugg acgaacugac gaacuucgag aaaggaaaga gcauuaccac aaaugccgau      180

agaacccuua aacggggaau gcgcagaaac cugcagcggu acaagcuccg cagagaaaca      240

cugaccgagg uccugaagga gcacaaacuc aucacagaag acaccauucu guccgagaau      300

ggcaaccgga cgaccuuuga aacauaucgc cuuagagcga aggcugugac cgaggagauc      360

ucucuggaag aguucgcccg gguucuccug augaucaaua aaaagcgcgg guacaaaagc      420

uccagaaagg ccaagggagu ggaagagggc acacuuauug auggcaugga caucgcucgg      480

gaacuguaua acaauaaccu caccccuggg gagcugugcc ugcaacuccu ggaugccggg      540

aaaaaguuuc uuccggacuu cuaccgcucu gaucugcaga augagcucga cagaauuugg      600

gaaaaacaga aggaguauua ccccgaaauc cugacggaug uccuuaaaga ggaacugcgg      660

ggcaagaaaa gagacgcugu gugggccauu ugugcgaagu auuuuguuug gaaggagaac      720

uacacagagu ggaauaaaga aaaggggaaa accgagcagc aagaacgcga gcauaagcuc      780

gaaggaaucu acagcaaacg gaagagagau gaggccaagc gcgagaaccu gcaguggcgg      840

gugaacggcc ugaaagaaaa gcucucccug gagcagcuug ucauuguguu ccaggaaaug      900

aauacacaaa ucaacaauag cucuggguau cugggagcua ucuccgacag aagcaaagag      960

cucuacuuua acaagcagac cgugggccag uaucagaugg agaugcugga caaaaauccu     1020

aacgccucuc uucgcaauau ggucuucuac cggcaagauu aucuggacga auuuaacaug     1080

cucugggaga agcaggcugu uuaccacaag gaacugaccg aggaacugaa aaaggagauu     1140

agagauauca uuaucuucua ucagcgccgg cucaaauccc aaaagggccu gauuggguuu     1200

ugcgaguucg aaagcagaca gaucgaggug gacaucgaug gcaaaaagaa gauuaaaaca     1260

gugggaaauc gcgucaucuc ucgguccagc ccccuuuuuc aggaguucaa gauuuggcag     1320

auccugaaca auauugaagu gacgguugug gggaaaaaga gaaaacggcg caagcucaaa     1380

gaaaacuacu ccgcccuguu cgaggagcug aaugacgcgg aacaacuuga gcucaacgga     1440

ucuagacggc ugugucagga agagaaggaa cugcucgccc aggagcuguu ugucagagau     1500

aagaugacca aaagcgaggu gcuuaagcuc cuguucgaca auccucagga acuggaucug     1560

aacuuuaaaa caaucgacgg caacaagacc ggguacgcuc uuuuccaagc cuauuccaaa     1620

augaucgaga ugagcggaca ugaacccguu gauuuuaaga agccugugga gaaagucgug     1680

gaguacauua aggcugucuu cgaccuccug aauuggaaca cagacauccu gggcuuuaau     1740

ucuaacgagg aacucgauaa ucaguccuau uacaaacugu ggcaccuucu guauagcuuc     1800

gaaggcgaca acaccccgac ggggaaugga cgccucauuc agaagaugac agagcuguac     1860

ggcuuugaaa aagaguaugc caccauccug gccaacgugu cuuuccagga ugacuacggg     1920

agccuuuccg cuaaggcgau ucauaagauc cucccccacc ugaaagaggg aaaucgguac     1980

gauguugccu gcguguaugc uggcuacaga cacucugaaa gcucccugac ccgcgaggaa     2040

auugccaaca agguccucaa agacagacug augcuucugc cuaagaauag ccuccauaac     2100

cccgugguug agaaaauccu gaaucaaaug gugaacguca ucaaugugau uaucgauauu     2160

uaugggaagc cugacgaaau ccggguggag cuggcccgcg agcuuaagaa aaacgcuaag     2220

gaaagagagg aacucacaaa aucuauugcc cagaccacaa aggcgcacga ggaauacaaa     2280

acgcugcugc agaccgaguu uggccucaca aacguuuccc ggaccgauau ccugcgcuau     2340

aaacuuuaca aagagcugga aagcugugga uauaagacac ucuacucuaa uaccuacauc     2400

uccagagaga agcuguucag caaagaauuu gacauugagc auaucauucc ccaggcucgg     2460

cuguucgaug acuccuucuc uaacaagacg cuugaggccc gcagcgucaa uaucgaaaaa     2520

ggcaacaaua ccgccuauga uuuugugaag gagaaauucg gggaauccgg agcugacaac     2580

ucucuggagc aauaccucaa uaacauugaa gaccuguuua agagcggcaa gaucuccaaa     2640

acaaaguaua auaaacucaa gauggccgag caggauaucc cggacggguu cauugagaga     2700

gaucugcgga acacccagua caucgcuaaa aaggcccuua gcaugcugaa ugaaauuucu     2760

caccgcgugg ucgcgacauc cggaagcgug accgacaagc ucagagagga uuggcaacug     2820

aucgacguua ugaaagaacu gaacugggag aaguauaaag cccuuggccu gguggaauac     2880

uuugaggauc gggacgggag acagauuggc cgcaucaagg auuggacgaa acggaaugac     2940

cacagacauc acgcuaugga ugcccucaca gucgcuuuca ccaaggacgu guuuauccag     3000

uauuucaaca auaagaacgc cucucuggau ccuaacgcga augagcaugc cauuaaaaac     3060

aaguacuuuc agaauggacg cgcuaucgcc cccaugccuc uccgggaguu cagagcugaa     3120

gccaaaaagc accuggaaaa cacacuuauu uccaucaaag ccaagaauaa gguuauuacc     3180

ggcaacauca auaaaacccg caagaaaggg ggagugaaca agaauaugca acagacaccc     3240

cggggccagc ugcaucucga gacgauuuac gggagcggaa aacaguaucu gaccaaggaa     3300

gagaaaguca acgcuucuuu ugacaugaga aagaucggca cagugagcaa guccgcguac     3360

cgcgacgccc ugcuuaaacg gcuguaugag aaugauaacg accccaagaa ggcuuucgcc     3420

gggaagaaua gccucgauaa acaaccuauc uggcuggaca aggaacagau gagaaagguu     3480

ccggagaaag ugaagauugu cacccucgaa gccaucuaca caauucggaa agagaucucu     3540

cccgaucuga agguggacaa agugauugau gucggcguuc gcaagauccu uaucgacaga     3600

cugaacgagu auggaaauga ugcuaagaaa gccuuuucca accuggacga aaauccuauu     3660

uggcucaaca aggagaaagg caucagcauu aagcggguga ccaucuccgg gauuucuaac     3720

gcgcagagcc ugcacgugaa aaaggauaag gacggaaaac ccauccuuga cgaaaauggc     3780

cgcaacaucc cugucgauuu cgugaauacg gggaacaauc accauguugc uguguacuau     3840

agacccguca uugacaagcg gggacagcug gugguugaug aggccggcaa cccgaaauac     3900

gaacucgagg agguggucgu guccuucuuu gaagccguua caagagcuaa ucuggggcaa     3960

ccuaucauug acaaggauua caaaaccacc gagggcuggc aguuccucuu uucuaugaag     4020

cagaacgaau auuucguguu ucccaaugag aaaacaggau ucaacccuaa ggaaaucgac     4080

cugcuugaug ucgagaauua cggccugauu agccccaacc uguuucgcgu gcaaaaguuc     4140

ucccucaaaa auuauguguu ucggcaccau cuggagacca caaucaagga cacgucuagc     4200

auucuuagag ggaucaccug gaucgauuuc cgcuccagca aaggacugga cacaauuguc     4260

aagguucggg ugaaccacau cggccagauu gugucugucg gggaauacug a              4311


<210>  4519
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4519
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acaaagauga gcgguccucu aucgucaagc ugggcguucg cgugaauccc      120

cucacagugg acgaacugac gaacuucgag aaaggaaaga gcauuaccac aaaugccgau      180

agaacccuua aacggggaau gcgcagaaac cugcagcggu acaagcuccg cagagaaaca      240

cugaccgagg uccugaagga gcacaaacuc aucacagaag acaccauucu guccgagaau      300

ggcaaccgga cgaccuuuga aacauaucgc cuuagagcga aggcugugac cgaggagauc      360

ucucuggaag aguucgcccg gguucuccug augaucaaua aaaagcgcgg guacaaaagc      420

uccagaaagg ccaagggagu ggaagagggc acacuuauug auggcaugga caucgcucgg      480

gaacuguaua acaauaaccu caccccuggg gagcugugcc ugcaacuccu ggaugccggg      540

aaaaaguuuc uuccggacuu cuaccgcucu gaucugcaga augagcucga cagaauuugg      600

gaaaaacaga aggaguauua ccccgaaauc cugacggaug uccuuaaaga ggaacugcgg      660

ggcaagaaaa gagacgcugu gugggccauu ugugcgaagu auuuuguuug gaaggagaac      720

uacacagagu ggaauaaaga aaaggggaaa accgagcagc aagaacgcga gcauaagcuc      780

gaaggaaucu acagcaaacg gaagagagau gaggccaagc gcgagaaccu gcaguggcgg      840

gugaacggcc ugaaagaaaa gcucucccug gagcagcuug ucauuguguu ccaggaaaug      900

aauacacaaa ucaacaauag cucuggguau cugggagcua ucuccgacag aagcaaagag      960

cucuacuuua acaagcagac cgugggccag uaucagaugg agaugcugga caaaaauccu     1020

aacgccucuc uucgcaauau ggucuucuac cggcaagauu aucuggacga auuuaacaug     1080

cucugggaga agcaggcugu uuaccacaag gaacugaccg aggaacugaa aaaggagauu     1140

agagauauca uuaucuucua ucagcgccgg cucaaauccc aaaagggccu gauuggguuu     1200

ugcgaguucg aaagcagaca gaucgaggug gacaucgaug ggaaaaagaa gauuaaaaca     1260

gugggcaauc gcgucaucuc ucgguccagc ccccuuuuuc aggaguucaa gauuuggcag     1320

auccugaaca auauugaagu gacgguugug gggaaaaaga gaaaacggcg caagcucaaa     1380

gaaaacuacu ccgcccuguu cgaggagcug aaugacgcgg aacaacuuga gcucaacgga     1440

ucuagacggc ugugucagga agagaaggaa cugcucgccc aggagcuguu uaucagagau     1500

aagaugacca aaagcgaggu ccuuaagcug cuuuucgaca auccucagga acuggaucug     1560

aacuuuaaaa caaucgacgg caacaagacc ggguacgcuc uuuuccaagc cuauuccaaa     1620

augauugaga ugagcggaca ugaacccgug gauuuuaaga agccuguuga gaaagugguc     1680

gaguacauca aggcuguguu cgaccuccug aauuggaaca cagacauucu gggcuuuaau     1740

ucuaacgagg aacucgauaa ucagccguau uacaaacugu ggcaccuucu guauuccuuc     1800

gaaggcgaca acacccccac ggggaaugga cgccucaucc agaagaugac agagcuguac     1860

ggcuuugaaa aagaguaugc caccauucug gccaacguca gcuuccagga ugacuacggg     1920

ucucuuagcg cuaaggcgau ccauaagauu cucccucacc ugaaagaggg aaaucgguac     1980

gauguggccu gcguuuaugc uggcuacaga cacuccgaau cuagccugac ccgcgaggaa     2040

aucgccaaca aggugcucaa agacagacug augcuucugc ccaaaaauuc ccuccauaac     2100

ccugucgugg agaagauccu gaaucaaaug guuaacguga uuaaugucau cauugauauc     2160

uaugggaagc ccgacgaaau ucggguggag cuggcccgcg agcuuaagaa aaacgcuaag     2220

gaaagagagg aacucacaaa aagcaucgcc cagaccacaa aggcgcacga ggaauacaaa     2280

acgcugcugc agaccgaguu uggccucaca aacgugucuc ggaccgauau ccugcgcuau     2340

aagcucuaca aagagcugga auccugugga uauaagacac ucuacagcaa uaccuacauu     2400

ucuagagaga agcuguucuc caaagaauuu gacaucgagc auauuauccc gcaggcucgg     2460

cuguucgaug acagcuucuc caacaagacg cuugaggccc gcucuguuaa uauugaaaaa     2520

ggcaacaaga ccgccuauga uuuugucaaa gagaaguucg gggaaagcgg agcugacaau     2580

ucccuggagc acuaccucaa caauaucgaa gaccuguuua agucuggcaa aaucagcaag     2640

acaaaauaua acaagcucaa aauggccgag caagauauuc cugacggguu caucgagaga     2700

gaucugcgga auacccagua cauugcuaag aaggcccuuu ccaugcugaa cgaaaucagc     2760

caccgcgugg uggcgacauc uggauccguc accgacaaac ucagagagga uuggcagcug     2820

auugacguga ugaaggaacu gaauugggag aaauauaagg cccuuggccu gguugaauac     2880

uuugaggauc gggacgggag acaaaucggc cgcaucaaag auuggacgaa gcggaacgac     2940

cauagacacc augcuaugga ugcccucaca guggcuuuca ccaaggacgu cuuuauucag     3000

uauuucaaua acaaaaaugc cagccuggau cccaacgcga acgagcacgc caucaagaau     3060

aaauacuuuc agaacggacg cgcuauugcc ccuaugcccc uccgggaguu cagagcugaa     3120

gccaagaaac aucuggaaaa uacacuuauc ucuauuaagg ccaagaacaa agugaucacc     3180

ggcaauauua acaagacccg caaaaaggga ggaguuaaua aaaacaugca gcaaacaccc     3240

cggggccagc ugcaccucga gacgaucuac ggguccggaa agcaguaucu gaccaaagaa     3300

gagaagguga augcuagcuu ugacaugaga aagaucggca cagucucuaa aagcgcguac     3360

cgcgacgccc ugcuuaagcg gcuguaugag aacgauaaug acccuaaaaa ggcuuucgcc     3420

gggaaaaacu cccucgauaa gcagccgauu uggcuggaca aggaacaaau gagaaaagug     3480

cccgagaagg uuaaaaucgu gacccucgaa gccauuuaca caauccggaa ggagauuagc     3540

ccugaucuga aagucgacaa ggugaucgau gugggcgucc gcaagauccu uauugacaga     3600

cugaaugagu auggaaacga ugcuaaaaag gccuuuucua aucuggacaa aaaccccauc     3660

uggcucaaua aggaaaaagg cauuuccauc aagcggguua ccauuagcgg gaucuccaac     3720

gcgcagucuc ugcacgugaa gaaagauaag gacggaaaac cuauccuuga cgagaacggc     3780

cgcaauauuc ccguggauuu cgucaacacg gggaauaacc aucacguggc uguuuacuau     3840

agaccgguga ucgacaagcg gggacagcug gucguggaug aagccggcaa uccuaaauac     3900

gagcucgaag agguuguggu cagcuucuuu gaggccguga caagagcuaa ccuggggcuc     3960

cccauuaucg acaaggauua caaaaccacc gaaggcuggc aguuccuguu uuccaugaag     4020

caaaaugagu auuucguuuu uccuaacgaa aagacaggau ucaaucccaa agagauugac     4080

cuucuggaug uggaaaacua cggccugauc ucuccuaauc ucuuucgcgu ccagaaguuc     4140

agccugaaaa acuauguguu ucggcaucac cuugagacca caauuaagga cacguccucu     4200

auccugagag ggaucaccug gauugauuuc cgcagcucca aaggacucga cacaaucgug     4260

aagguccggg uuaaucacau uggccagauc gugagcgugg gggaguacug a              4311


<210>  4520
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4520
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acaaagauga gcgguccucu aucgucaagc ugggcguucg cgugaauccc      120

cucacagugg acgaacugac gaacuucgag aaaggaaaga gcauuaccac aaaugccgau      180

agaacccuua aacggggaau gcgcagaaac cugcagcggu acaagcuccg cagagaaaca      240

cugaccgagg uccugaagga gcacaaacuc aucacagaag acaccauucu guccgagaau      300

ggcaaccgga cgaccuuuga aacauaucgc cuuagagcga aggcugugac cgaggagauc      360

ucucuggaag aguucgcccg gguucuccug augaucaaua aaaagcgcgg guacaaaagc      420

uccagaaagg ccaagggagu ggaagagggc acacuuauug auggcaugga caucgcucgg      480

gaacuguaua acaauaaccu caccccuggg gagcugugcc ugcaacuccu ggaugccggg      540

aaaaaguuuc uuccggacuu cuaccgcucu gaucugcaga augagcucga cagaauuugg      600

gaaaaacaga aggaguauua ccccgaaauc cugacggaug uccuuaaaga ggaacugcgg      660

ggcaagaaaa gagacgcugu gugggccauu ugugcgaagu auuuuguuug gaaggagaac      720

uacacagagu ggaauaaaga aaaggggaaa accgagcagc aagaacgcga gcauaagcuc      780

gaaggaaucu acagcaaacg gaagagagau gaggccaagc gcgagaaccu gcaguggcgg      840

gugaacggcc ugaaagaaaa gcucucccug gagcagcuug ucauuguguu ccaggaaaug      900

aauacacaaa ucaacaauag cucuggguau cugggagcua ucuccgacag aagcaaagag      960

cucuacuuua acaagcagac cgugggccag uaucagaugg agaugcugga caaaaauccu     1020

aacgccucuc uucgcaauau ggucuucuac cggcaagauu aucuggacga auuuaacaug     1080

cucugggaga agcaggcugu uuaccacaag gaacugaccg aggaacugaa aaaggagauu     1140

agagauauca uuaucuucua ucagcgccgg cucaaauccc aaaagggccu gauuggguuu     1200

ugcgaguucg aaagcagaca gaucgaggug gacaucgaug ggaaaaagaa gauuaaaaca     1260

gugggcaauc gcgucaucuc ucgguccagc ccccuuuuuc aggaguucaa gauuuggcag     1320

auccugaaca auauugaagu gacgguugug gggaaaaaga gaaaacggcg caagcucaaa     1380

gaaaacuacu ccgcccuguu cgaggagcug aaugacgcgg aacaacuuga gcucaacgga     1440

ucuagacggc ugugucagga agagaaggaa cugcucgccc aggagcuguu uaucagagau     1500

aagaugacca aaagcgaggu ccuuaagcug cuuuucgaca auccucagga acuggaucug     1560

aacuuuaaaa caaucgacgg caacaagacc ggguacgcuc uuuuccaagc cuauuccaaa     1620

augauugaga ugagcggaca ugaacccgug gauuuuaaga agccuguuga gaaagugguc     1680

gaguacauca aggcuguguu cgaccuccug aauuggaaca cagacauucu gggcuuuaau     1740

ucuaacgagg aacucgauaa ucaguccuau uacaaacugu ggcaccuucu guauagcuuc     1800

gaaggcgaca acaccccgac ggggaaugga cgccucaucc agaagaugac agagcuguac     1860

ggcuuugaaa aagaguaugc caccauucug gccaacgucu cuuuccagga ugacuacggg     1920

agccuuuccg cuaaggcgau ccauaagauu cucccccacc ugaaagaggg aaaucgguac     1980

gauguggccu gcguuuaugc uggcuacaga cacucugaaa gcucccugac ccgcgaggaa     2040

aucgccaaca aggugcucaa agacagacug augcuucugc cuaagaauag ccuccauaac     2100

cccgucgugg agaaaauccu gaaucaaaug guuaacguga uuaaugucau cauugauauc     2160

uaugggaagc cugacgaaau ucggguggag cuggcccgcg agcuuaagaa aaacgcuaag     2220

gaaagagagg aacucacaaa aucuaucgcc cagaccacaa aggcgcacga ggaauacaaa     2280

acgcugcugc agaccgaguu uggccucaca aacguguccc ggaccgauau ccugcgcuau     2340

aagcucuaca aagagcugga aagcugugga uauaagacac ucuacucuaa uaccuacauu     2400

uccagagaga agcuguucag caaagaauuu gacaucgagc auauuauccc ccaggcucgg     2460

cuguucgaug acuccuucuc uaacaagacg cuugaggccc gcagcguuaa uauugaaaaa     2520

ggcaacaaga ccgccuauga uuuugucaaa gagaaguucg gggaauccgg agcugacaau     2580

ucucuggagc acuaccucaa caauaucgaa gaccuguuua agagcggcaa aaucuccaag     2640

acaaaauaua acaagcucaa aauggccgag caagauauuc cggacggguu caucgagaga     2700

gaucugcgga auacccagua cauugcuaag aaggcccuua gcaugcugaa cgaaaucucu     2760

caccgcgugg uggcgacauc cggaagcguc accgacaaac ucagagagga uuggcagcug     2820

auugacguga ugaaggaacu gaauugggag aaauauaagg cccuuggccu gguugaauac     2880

uuugaggauc gggacgggag acaaaucggc cgcaucaaag auuggacgaa gcggaacgac     2940

cauagacacc augcuaugga ugcccucaca guggcuuuca ccaaggacgu cuuuauucag     3000

uauuucaaua acaaaaaugc cucucuggau ccuaacgcga acgagcacgc caucaagaau     3060

aaauacuuuc agaacggacg cgcuauugcc cccaugccuc uccgggaguu cagagcugaa     3120

gccaagaaac aucuggaaaa uacacuuauc uccauuaagg ccaagaacaa agugaucacc     3180

ggcaauauua acaagacccg caaaaaggga ggaguuaaua aaaacaugca gcaaacaccc     3240

cggggccagc ugcaccucga gacgaucuac gggagcggaa agcaguaucu gaccaaagaa     3300

gagaagguga augcuucuuu ugacaugaga aagaucggca cagucagcaa acuggcguac     3360

cgcgacgccc uucugaagcg gcucuaugag aacgauaaug accccaaaaa ggcuuucgcc     3420

gggaaaaacu cccuggauaa gcagccuauu uggcucgaca aggaacaaau gagaaaagug     3480

ccggagaagg uuaaaaucgu gacccuggaa gccauuuaca caauccggaa ggagauuagc     3540

cccgaucuua aagucgacaa ggugaucgau gugggcgucc gcaagauccu gauugacaga     3600

cugaaugagu auggaaacga ugcuaaaaag gccuuuucua aucucgacga aaacccuauc     3660

uggcugaaua aagagaaggg cauuuccauc aaacggguua ccauuagcgg gaucuccaac     3720

gcgcagucuc uucacgugaa gaaggauaaa gacggaaagc ccauccugga cgaaaacggc     3780

cgcaauauuc cuguggauuu cgucaacacg gggaauaacc aucacguggc uguuuacuau     3840

agacccguga ucgacaaacg gggacagcuc gucguggaug aggccggcaa uccgaaguac     3900

gaacuggagg agguuguggu cagcuucuuu gaagccguga caagagcuaa ccucgggcag     3960

ccuauuaucg acaaagauua caagaccacc gagggcuggc aauuccuguu uuccaugaaa     4020

cagaaugaau auuucguuuu ucccaacgag aagacaggau ucaauccuaa ggaaauugac     4080

cuucuggaug uggagaacua cggccugauc ucucccaauc ucuuucgcgu ccagaaauuc     4140

agccugaaga acuauguguu ucggcaucac cuugagacca caauuaaaga cacguccucu     4200

auccugagag ggaucaccug gauugauuuc cgcagcucca agggacucga cacaaucgug     4260

aaaguccggg uuaaucacau uggccaaauc gugagcgugg gggaauacug a              4311


<210>  4521
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4521
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acaaagauga gcgguccucu aucgucaagc ugggcguucg cgugaauccc      120

cucacagugg acgaacugac gaacuucgag aaaggaaaga gcauuaccac aaaugccgau      180

agaacccuua aacggggaau gcgcagaaac cugcagcggu acaagcuccg cagagaaaca      240

cugaccgagg uccugaagga gcacaaacuc aucacagaag acaccauucu guccgagaau      300

ggcaaccgga cgaccuuuga aacauaucgc cuuagagcga aggcugugac cgaggagauc      360

ucucuggaag aguucgcccg gguucuccug augaucaaua aaaagcgcgg guacaaaagc      420

uccagaaagg ccaagggagu ggaagagggc acacuuauug auggcaugga caucgcucgg      480

gaacuguaua acaauaaccu caccccuggg gagcugugcc ugcaacuccu ggaugccgga      540

aagaaguuuc uuccggacuu cuaccgcucu gaucugcaga augagcucga cagaauuugg      600

gaaaaacaga aggaguauua ccccgaaauc cugacggaug uccuuaaaga ggaacugcgg      660

ggcaagaaaa gagacgcugu gugggccauu ugugcgaagu auuuuguuug gaaggagaca      720

uacaccgagu ggaacaaaga aaaggggaaa acagagcagc aagaacgcga gcauaagcuc      780

gaaggaaucu acagcaaacg gaagagagau gaggccaagc gcgagaaucu gcaguggcgg      840

gugaacggcc ugaaagaaaa gcucucccug gagcagcuug ucauuguguu ccaggaaaug      900

aacacccaaa ucaauaacag cucuggguau cugggagcua ucuccgacag aagcaaagag      960

cucuacuuua auaagcagac cgugggccag uaucagaugg agaugcugga caaaaacccu     1020

aaugccucuc uucgcaacau ggucuucuac cggcaagauu aucuggacga auuuaauaug     1080

cucugggaga agcaggcugu uuaccacaag gaacugacag aggaacugaa aaaggagauu     1140

agagauauca uuaucuucua ucagcgccgg cucaaauccc aaaagggccu gauuggguuu     1200

ugcgaguucg aaagcagaca gaucgaggug gacaucgaug ggaaaaagaa gauuaaaacg     1260

gugggcaacc gcgucaucuc ucgguccagc ccccuuuuuc aggaguucaa gauuuggcag     1320

auccugaaua acauugaggu gaccguugug gggaaaaaga gaaaacggcg caagcucaaa     1380

gaaaauuacu ccgccaucuu cgaggagcug aacgacgcgg aacaacugga gcuuaaugga     1440

ucuagacggc ucugucagga agagaaggaa cugcuggccc aggagcucuu ugucagagau     1500

aagaugacaa aaagcgaggu gcugaagcug cuguucgaca acccucagga acucgaucug     1560

aauuuuaaaa ccaucgacgg caacaagaca ggguacgcuc uguuccaagc cuauuccaaa     1620

augauugaga ugagcggaca ugaacccguu gauuuuaaga agccugugga gaaagucgug     1680

gaguacauca aggcugucuu cgaccuucuc aacuggaaua ccgacauucu gggcuuuaac     1740

ucuaaugaag aacuggauaa ccaguccuau uacaaacucu ggcaccuucu uuauagcuuc     1800

gaaggcgaca auacgccgac agggaacgga cgccugaucc agaagaugac cgagcucuac     1860

ggcuuugaaa aagaguaugc caccauucug gccaaugugu cuuuccagga ugacuacggg     1920

agccuguccg cuaaggcgau ccauaagauu cuuccccacc ucaaagaggg aaaccgguac     1980

gauguugccu gcguguaugc uggcuacaga cacucugaaa gcucccugac acgcgaggaa     2040

aucgccaaua agguccugaa agacagacuc augcugcuuc cuaagaacag ccugcauaau     2100

cccgugguug agaaaauccu caaccaaaug gugaauguca uuaacgugau cauugauauc     2160

uaugggaagc cugacgaaau ucggguggag cuggcccgcg agcugaagaa aaaugcuaag     2220

gaaagagagg aacuuaccaa aucuaucgcc cagacaacga aggcgaacga ggaauacaaa     2280

acccuccugc agacagaguu uggccugacc aacguuuccc ggacagauau ccuccgcuau     2340

aagcuguaca aagagcuuga aagcugugga uauaagaccc uguacucuaa uacguacauu     2400

uccagagaga aacucuucag caaagaauuu gacaucgagc acauuauccc ccaggcucgg     2460

cuguucgaug acuccuucuc uaacaagacc cuggaggccc gcagcgucaa uauugaaaaa     2520

ggcaacaaga cagccuauga uuuugugaaa gagaaguucg gggaauccgg agcugacaau     2580

ucucuugagc auuaccugaa caauaucgaa gaccucuuua agagcggcaa aaucuccaag     2640

accaaauaua acaagcugaa aauggccaag caagauauuc cggacggguu caucgagaga     2700

gaucuccgga auacacagua cauugcuaag aaagcccuga gcaugcuuaa cgagaucucu     2760

caccgcgugg ucgcgaccuc cggaagcgug acggacaagc ugagagaaga uuggcagcuc     2820

auugacguua ugaaagagcu gaauugggaa aaguauaaag cccugggccu uguggaguac     2880

uuugaagauc gggacgggag acaaaucggc cgcaucaagg auuggacaaa gcggaacgac     2940

cacagacauc acgcuaugga ugcccugacc gucgcuuuca caaaagacgu guuuauucag     3000

uauuucaaua acaagaaugc cucucucgau ccuaacgcga acgagcaugc caucaaaaau     3060

aaguacuuuc agaacggacg cgcuauugcc cccaugccuc ugcgggaguu cagagcugaa     3120

gccaaaaagc accucgagaa uacccugauc uccauuaagg ccaaaaacaa gguuaucacc     3180

ggcaauauua acaaaacacg caagaaaggg ggagugaaua agaacaugca gcaaacgccc     3240

cggggccagc uucaucugga aaccaucuac gggagcggaa aacaguaucu cacaaaggag     3300

gaaagaguca augcuucuuu ugacaugcgc aagaucgaga ccgugagcaa auccgcguac     3360

cgggacgccc ugcugaagag acuuuaugag aacgauaaug accccaaaaa ggcuuucgcc     3420

ggcaaaaaca gccuggauaa gcagccuauu uggcucgaca aggaacaaaa acggaagguu     3480

ccggagaaag ugaagaucgu cacacuggaa gccauuuaca ccauccgcaa agagauuucu     3540

cccgaucuca agguggacaa ggugaucgau gucgggguua gaaaaauccu gauugaccgg     3600

cuuaaugagu auggcaacga ugcuaagaaa gccuuuucca aucuggacga aaacccuauc     3660

uggcugaaua aggagaaagg aauuagcauc aagcgcguga cgauuuccgg caucucuaac     3720

gcgcagagcc uccacgugaa gaaagauaag gacgggaaac ccauccugga cgaaaacgga     3780

agaaauauuc cugucgauuu cgugaacaca ggcaauaacc accauguugc uguguacuau     3840

cggcccguca ucgacaagag agggcagcuu gugguugaug aggccggaaa uccgaaauac     3900

gaacuggagg agguggucgu guccuucuuu gaagccguua cccgcgcuaa ccucggccag     3960

ccuauuaucg acaaggauua caaaaccaca gagggguggc aauuccuguu uucuaugaag     4020

cagaaugaau auuucguguu ucccaacgag aagaccggcu ucaauccuaa agaaauugac     4080

cuccuggaug ucgagaacua cggacuuauc agccccaauc uguuucgggu gcagaaguuc     4140

ucccugaaaa acuauguguu uagacaccau cucgagacaa cgauuaagga caccucuagc     4200

auccugcgcg gcaucacaug gauugauuuc cgguccagca aagggcuuga caccaucguc     4260

aagguuagag ugaaucacau uggacaaauc gugucugucg gcgaauacug a              4311


<210>  4522
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4522
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acaaagauga gcgguccucu aucgucaagc ugggcguucg cgugaauccc      120

cucacagugg acgaacugac gaacuucgag aaaggaaaga gcauuaccac aaaugccgau      180

agaacccuua aacggggaau gcgcagaaac cugcagcggu acaagcuccg cagagaaaca      240

cugaccgagg uccugaagga gcacaaacuc aucacagaag acaccauucu guccgagaau      300

ggcaaccgga cgaccuuuga aacauaucgc cuuagagcga aggcugugac cgaggagauc      360

ucucuggaag aguucgcccg gguucuccug augaucaaua aaaagcgcgg guacaaaagc      420

uccagaaagg ccaagggagu ggaagagggc acacuuauug auggcaugga caucgcucgg      480

gaacuguaua acaauaaccu caccccuggg gagcugugcc ugcaacuccu ggaugccggg      540

aaaaaguuuc uuccggacuu cuaccgcucu gaucugcaga augagcucga cagaauuugg      600

gaaaaacaga aggaguauua ccccgaaauc cugacggaug uccuuaaaga ggaacugcgg      660

ggcaagaaaa gagacgcugu gugggccauu ugugcgaagu auuuuguuug gaaggagaca      720

uacaccgagu ggaacaaaga aaaggggaaa acagagcagc aagaaaagga gcauaaacuc      780

gaaggaaucu acagcaagcg caagcgggau gaggccaaaa gagagaaucu gcaguggcgc      840

gugaacggcc ugaaggaaaa acucucccug gagcagcuug ucauuguguu ccaggaaaug      900

aacacccaaa ucaauaacag cucuggguau cugggagcua ucuccgaccg gagcaaggag      960

cucuacuuua auaaacagac cgugggccag uaucagaugg agaugcugga caagaacccu     1020

aaugccucuc uuagaaacau ggucuucuac cgccaagauu aucuggacga auuuaauaug     1080

cucugggaga agcaggcugu uuaccacaaa gaacugacag aggaacugaa gaaagagauu     1140

cgggauauca uuaucuucua ucagagacgc cucaaguccc aaaaaggccu gauuggguuu     1200

ugcgaguucg aaagccggca gaucgaggug gacaucgaug gaaagaagaa aauuaagacg     1260

gugggcaaca gagucaucuc ucgcuccagc ccccuuuuuc aggaguucaa aauuuggcag     1320

auccugaaua acauugaagu gaccguugug gggaagaaac ggaagagacg gaaacucaag     1380

gaaaauuacu ccgcccuguu cgaggagcug aacgacgcgg aacaacuuga gcucaaugga     1440

ucucgcagac ugugucagga agagaaggaa cugcucgccc aggagcuguu uauccgggau     1500

aaaaugacaa agagcgaggu ccuuaaacuc cuguucgaca acccucagga acuggaucug     1560

aauuuuaaga ccaucgacgg caacaaaaca ggguacgcuc uuuuccaagc cuauuccaag     1620

augauugaga ugagcggaca ugaacccgug gauuuuaaga aaccuguuga gaaggugguc     1680

gaguacauca aagcuguguu cgaccuccug aacuggaaua ccgacauucu gggcuuuaac     1740

ucuaaugagg aacucgauaa ccaguccuau uacaagcugu ggcaccuucu guauagcuuc     1800

gaaggcgaca auacgccgac agggaacgga agacucaucc agaaaaugac cgagcuguac     1860

ggcuuugaaa aggaguaugc caccauucug gccaaugucu cuuuccagga ugacuacggg     1920

agccuuuccg cuaaggcgau ccauaaaauu cucccccacc ugaaggaggg aaaccgcuac     1980

gauguggccu gcguuuaugc uggcuaccgg cacucugaaa gcucccugac aagagaggaa     2040

aucgccaaua aagugcucaa ggaccgccug augcuucugc cuaaaaacag ccuccauaau     2100

cccgucgugg agaagauccu gaaccaaaug guuaauguga uuaacgucau cauugauauc     2160

uaugggaagc cugacgaaau uagaguggag cuggcccggg agcuuaaaaa gaaugcuaaa     2220

gaacgcgagg aacucaccaa gucuaucgcc cagacaacga aagcgcacga ggaauacaag     2280

acccugcugc agacagaguu uggccucacc aacgugucca gaacagauau ccugcgguau     2340

aaacuuuaca aggagcugga aagcugugga uauaagaccc ucuacucuaa cacguacauu     2400

ucccgcgaga aacuguucag caaggaauuu gacaucgagc auauuauccc ccaggcuaga     2460

cuguucgaug acuccuucuc uaauaaaacc cuugaggccc ggagcguuaa cauugaaaag     2520

ggcaauaaaa cagccuauga uuuugucaag gagaaguucg gggaauccgg agcugacaac     2580

ucucuggagc acuaccucaa uaacaucgaa gaccuguuua aaagcggcaa gaucuccaaa     2640

accaaguaua auaaacucaa gauggccgag caagauauuc cggacggguu caucgagcgc     2700

gaucugagaa acacacagua cauugcuaag aaagcccuua gcaugcugaa ugaaaucucu     2760

caccgggugg uggcgaccuc cggaagcguc acggacaagc uccgcgagga uuggcagcug     2820

auugacguga ugaaagaacu gaacugggag aaguauaaag cccuuggccu gguugaauac     2880

uuugaggaua gagacgggcg gcaaaucggc agaaucaagg auuggacaaa gcgcaaugac     2940

caucggcacc augcuaugga ugcccucacc guggcuuuca caaaagacgu cuuuauucag     3000

uauuucaaca auaagaacgc cucucuggau ccuaaugcga acgagcacgc caucaaaaac     3060

aaguacuuuc agaauggcag agcuauugcc cccaugccuc uccgcgaguu ccgggcugaa     3120

gccaaaaagc aucuggaaaa cacccuuauc uccauuaagg ccaaaaauaa ggugaucacc     3180

ggaaacauua auaaaacaag aaagaaaggg ggaguuaaca agaauaugca gcaaacgccc     3240

cgcggccagc ugcaccucga gaccaucuac gggagcggaa aacaguaucu gacaaaggaa     3300

gagaagguga acgcuucuuu ugacaugcgg aaaaucggca ccgucagcaa gcuggcguac     3360

agagacgccc uucugaaacg ccucuaugag aaugauaacg accccaagaa ggcuuucgcc     3420

gggaaaaauu cccuggauaa gcagccuauu uggcucgaca aagaacaaau gcggaaggug     3480

ccggagaaag uuaagaucgu gacacuggaa gccauuuaca ccaucagaaa agagauuagc     3540

cccgaucuua aggucgacaa ggugaucgau gugggcgucc ggaaaauccu gauugaccgc     3600

cugaacgagu auggaaauga ugcuaagaaa gccuuuucua accucgacga aaauccuauc     3660

uggcugaaca aggagaaagg cauuuccauc aagagaguua cgauuagcgg gaucuccaau     3720

gcgcagucuc uucacgugaa gaaagauaag gacggaaaac ccauccugga cgaaaacggc     3780

cggaacauuc cuguggauuu cgucaauaca gggaacaauc aucacguggc uguuuacuau     3840

cgccccguga ucgacaagag aggacagcuc gucguggaug aggccggcaa cccgaaauac     3900

gaacuggagg agguuguggu cagcuucuuu gaagccguga cccgggcuaa ucucgggcag     3960

ccuauuaucg acaaggauua caaaaccaca gagggcuggc aauuccuguu uuccaugaag     4020

cagaacgaau auuucguuuu ucccaaugag aagaccggau ucaacccuaa agaaauugac     4080

cuucuggaug uggagaauua cggccugauc ucucccaacc ucuuuagagu ccagaaguuc     4140

agccugaaaa auuauguguu ucgccaucac cuugagacaa cgauuaagga caccuccucu     4200

auccugcggg ggaucacaug gauugauuuc agaagcucca aaggacucga caccaucgug     4260

aagguccgcg uuaaccacau uggccaaauc gugagcgugg gggaauacug a              4311


<210>  4523
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4523
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acaaagauga gcgguccucu aucgucaagc ugggcguucg cgugaauccc      120

cucacagugg acgaacugac gaacuucgag aaaggaaaga gcauuaccac aaaugccgau      180

agaacccuua aacggggaau gcgcagaaac cugcagcggu acaagcuccg cagagaaaca      240

cugaccgagg uccugaagga gcacaaacuc aucacagaag acaccauucu guccgagaau      300

ggcaaccgga cgaccuuuga aacauaucgc cuuagagcga aggcugugac cgaggagauc      360

ucucuggaag aguucgcccg gguucuccug augaucaaua aaaagcgcgg guacaaaagc      420

uccagaaagg ccaagggagu ggaagagggc acacuuauug auggcaugga caucgcucgg      480

gaacuguaua acaauaaccu caccccuggg gagcugugcc ugcaacuccu ggaugccggg      540

aaaaaguuuc uuccggacuu cuaccgcucu gaucugcaga augagcucga cagaauuugg      600

gaaaaacaga aggaguauua ccccgaaauc cugacggaug uccuuaaaga ggaacugcgg      660

ggcaagaaaa gagacgcugu gugggccauu ugugcgaagu auuuuguuug gaaggagaac      720

uacacagagu ggaauaaaga aaaggggaaa accgagcagc aagaacgcga gcauaagcuc      780

gaaggaaucu acagcaaacg gaagagagau gaggccaagc gcgagaaccu gcaguggcgg      840

gugaacggcc ugaaagaaaa gcucucccug gagcagcuug ucauuguguu ccaggaaaug      900

aauacacaaa ucaacaauag cucuggguau cugggagcua ucuccgacag aagcaaagag      960

cucuacuuua acaagcagac cgugggccag uaucagaugg agaugcugga caaaaauccu     1020

aacgccucuc uucgcaauau ggucuucuac cggcaagauu aucuggacga auuuaacaug     1080

cucugggaga agcaggcugu uuaccacaag gaacugaccg aggaacugaa aaaggagauu     1140

agagauauca uuaucuucua ucagcgccgg cucaaauccc aaaagggccu gauuggguuu     1200

ugcgaguucg aaagcagaca gaucgaggug gacaucgaug ggaaaaagaa gauuaaaaca     1260

gugggcaauc gcgucaucuc ucgguccagc ccccuuuuuc aggaguucaa gauuuggcag     1320

auccugaaca auauugaagu gacgguugug gggaaaaaga gaaaacggcg caagcucaaa     1380

gaaaacuacu ccgcccuguu cgaggagcug aaugacgcgg aacaacuuga gcucaacgga     1440

ucuagacggc ugugucagga agagaaggaa cugcucgccc aggagcuguu uaucagagau     1500

aagaugacca aaagcgaggu ccuuaagcug cuuuucgaca auccucagga acuggaucug     1560

aacuuuaaaa caaucgacgg caacaagacc ggguacgcuc uuuuccaagc cuauuccaaa     1620

augauugaga ugagcggaca ugaacccgug gauuuuaaga agccuguuga gaaagugguc     1680

gaguacauca aggcuguguu cgaccuccug aauuggaaca cagacauucu gggcuuuaau     1740

ucuaacgagg aacucgauaa ucagccguau uacaaacugu ggcaccuucu guauuccuuc     1800

gaaggcgaca acacccccac ggggaaugga cgccucaucc agaagaugac agagcuguac     1860

ggcuuugaaa aagaguaugc caccauucug gccaacguca gcuuccagga ugacuacggg     1920

ucucuuagcg cuaaggcgau ccauaagauu cucccucacc ugaaagaggg aaaucgguac     1980

gauguggccu gcguuuaugc uggcuacaga cacuccgaau cuagccugac ccgcgaggaa     2040

aucgccaaca aggugcucaa agacagacug augcuucugc ccaaaaauuc ccuccauaac     2100

ccugucgugg agaagauccu gaaucaaaug guuaacguga uuaaugucau cauugauauc     2160

uaugggaagc ccgacgaaau ucggguggag cuggcccgcg agcuuaagaa aaacgcuaag     2220

gaaagagagg aacucacaaa aagcaucgcc cagaccacaa aggcgcacga ggaauacaaa     2280

acgcugcugc agaccgaguu uggccucaca aacgugucuc ggaccgauau ccugcgcuau     2340

aagcucuaca aagagcugga auccugugga uauaagacac ucuacagcaa uaccuacauu     2400

ucuagagaga agcuguucuc caaagaauuu gacaucgagc auauuauccc gcaggcucgg     2460

cuguucgaug acagcuucuc caacaagacg cuugaggccc gcucuguuaa uauugaaaaa     2520

ggcaacaaga ccgccuauga uuuugucaaa gagaaguucg gggaaagcgg agcugacaau     2580

ucccuggagc acuaccucaa caauaucgaa gaccuguuua agucuggcaa aaucagcaag     2640

acaaaauaua acaagcucaa aauggccgag caagauauuc cugacggguu caucgagaga     2700

gaucugcgga auacccagua cauugcuaag aaggcccuuu ccaugcugaa cgaaaucagc     2760

caccgcgugg uggcgacauc uggauccguc accgacaaac ucagagagga uuggcagcug     2820

auugacguga ugaaggaacu gaauugggag aaauauaagg cccuuggccu gguugaauac     2880

uuugaggauc gggacgggag acaaaucggc cgcaucaaag auuggacgaa gcggaacgac     2940

cauagacacc augcuaugga ugcccucaca guggcuuuca ccaaggacgu cuuuauucag     3000

uauuucaaua acaaaaaugc cagccuggau cccaacgcga acgagcacgc caucaagaau     3060

aaauacuuuc agaacggacg cgcuauugcc ccuaugcccc uccgggaguu cagagcugaa     3120

gccaagaaac aucuggaaaa uacacuuauc ucuauuaagg ccaagaacaa agugaucacc     3180

ggcaauauua acaagacccg caaaaaggga ggaguuaaua aaaacaugca gcaaacaccc     3240

cggggccagc ugcaccucga gacgaucuac ggguccggaa agcaguaucu gaccaaagaa     3300

gagaagguga augcuagcuu ugacaugaga aagaucggca cagucucuaa aagcgcguac     3360

cgcgacgccc ugcuuaagcg gcuguaugag aacgauaaug acccuaaaaa ggcuuucgcc     3420

gggaaaaacu cccucgauaa gcagccgauu uggcuggaca aggaacaaau gagaaaagug     3480

cccgagaagg uuaaaaucgu gacccucgaa gccauuuaca caauccggaa ggagauuagc     3540

ucugaucuga aagucgacaa ggugaucgau gugggcgucc gcaagauccu uauugacaga     3600

cugaaugagu auggaaacga ugcuaaaaag gccuuuucca aucuggacaa aaacccuauc     3660

uggcucaaua aggaaaaagg cauuagcauc aagcggguua ccauuuccgg gaucucuaac     3720

gcgcagagcc ugcacgugaa gaaagauaag gacggaaaac ccauccuuga cgagaacggc     3780

cgcaauauuc cuguggauuu cgucaacacg gggaauaacc aucacguggc uguuuacuau     3840

agacccguga ucgacaagcg gggacagcug gucguggaug aagccggcaa uccgaaauac     3900

gagcucgaag agguuguggu cuccuucuuu gaggccguga caagagcuaa ccuggggcuc     3960

ccuauuaucg acaaggauua caaaaccacc gaaggcuggc aguuccuguu uucuaugaag     4020

caaaaugagu auuucguuuu ucccaacgaa aagacaggau ucaauccuaa agagauugac     4080

cuucuggaug uggaaaacua cggccugauc agccccaauc ucuuucgcgu ccagaaguuc     4140

ucccugaaaa acuauguguu ucggcaucac cuugagacca caauuaagga cacgucuagc     4200

auccugagag ggaucaccug gauugauuuc cgcuccagca aaggacucga cacaaucgug     4260

aagguccggg uuaaucacau uggccagauc gugucugugg gggaguacug a              4311


<210>  4524
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4524
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acaaagauga gcgguccucu aucgucaagc ugggcguucg cgugaauccc      120

cucacagugg acgaacugac gaacuucgag aaaggaaaga gcauuaccac aaaugccgau      180

agaacccuua aacggggaau gcgcagaaac cugcagcggu acaagcuccg cagagaaaca      240

cugaccgagg uccugaagga gcacaaacuc aucacagaag acaccauucu guccgagaau      300

ggcaaccgga cgaccuuuga aacauaucgc cuuagagcga aggcugugac cgaggagauc      360

ucucuggaag aguucgcccg gguucuccug augaucaaua aaaagcgcgg guacaaaagc      420

uccagaaagg ccaagggagu ggaagagggc acacuuauug auggcaugga caucgcucgg      480

gaacuguaua acaauaaccu caccccuggg gagcugugcc ugcaacuccu ggaugccggg      540

aaaaaguuuc uuccggacuu cuaccgcucu gaucugcaga augagcucga cagaauuugg      600

gaaaaacaga aggaguauua ccccgaaauc cugacggaug uccuuaaaga ggaacugcgg      660

ggcaagaaaa gagacgcugu gugggccauu ugugcgaagu auuuuguuug gaaggagaac      720

uacacagagu ggaauaaaga aaaggggaaa accgagcagc aagaacgcga gcauaagcuc      780

gaaggaaucu acagcaaacg gaagagagau gaggccaagc gcgagaaccu gcaguggcgg      840

gugaacggcc ugaaagaaaa gcucucccug gagcagcuug ucauuguguu ccaggaaaug      900

aauacacaaa ucaacaauag cucuggguau cugggagcua ucuccgacag aagcaaagag      960

cucuacuuua acaagcagac cgugggccag ugccagaugg agaugcugga caaaaauccu     1020

aacgccucuc uucgcaauau ggucuucuau cggcaagauu accuggacga auuuaacaug     1080

cucugggaga agcaggcugu uuaucacaag gaacugaccg aggaacugaa aaaggagauu     1140

agagauauca uuaucuucua ccagcgccgg cucaaauccc aaaagggccu gauuggguuu     1200

ugugaguucg aaagcagaca gaucgaggug gacaucgaug ggaaaaagaa gauuaaaaca     1260

gugggcaauc gcgucaucuc ucgguccagc ccccuuuuuc aggaguucaa gauuuggcag     1320

auccugaaca auauugaagu gacgguugug gggaaaaaga gaaaacggcg caagcucaaa     1380

gaaaacuauu ccgcccuguu cgaggagcug aaugacgcgg aacaacuuga gcucaacgga     1440

ucuagacggc ugugccagga agagaaggaa cugcucgccc aggagcuguu uaucagagau     1500

aagaugacca aaagcgaggu ccuuaagcug cuuuucgaca auccucagga acuggaucug     1560

aacuuuaaaa caaucgacgg caacaagacc ggguacgcuc uuuuccaagc cuacuccaaa     1620

augauugaga ugagcggaca ugaacccgug gauuuuaaga agccuguuga gaaagugguc     1680

gaguauauca aggcuguguu cgaccuccug aauuggaaca cagacauucu gggcuuuaau     1740

ucuaacgagg aacucgauaa ucagccguac uauaaacugu ggcaccuucu guacuccuuc     1800

gaaggcgaca acacccccac ggggaaugga cgccucaucc agaagaugac agagcuguau     1860

ggcuuugaaa aagaguacgc caccauucug gccaacguca gcuuccagga ugacuauggg     1920

ucucuuagcg cuaaggcgau ccauaagauu cucccucacc ugaaagaggg aaaucgguac     1980

gauguggccu guguuuacgc uggcuauaga cacuccgaau cuagccugac ccgcgaggaa     2040

aucgccaaca aggugcucaa agacagacug augcuucugc ccaaaaauuc ccuccauaac     2100

ccugucgugg agaagauccu gaaucaaaug guuaacguga uuaaugucau cauugauauc     2160

uacgggaagc ccgacgaaau ucggguggag cuggcccgcg agcuuaagaa aaacgcuaag     2220

gaaagagagg aacucacaaa aagcaucgcc cagaccacaa aggcgcacga ggaauauaaa     2280

acgcugcugc agaccgaguu uggccucaca aacgugucuc ggaccgauau ccugcgcuac     2340

aagcucuaua aagagcugga auccugcgga uacaagacac ucuauagcaa uaccuacauu     2400

ucuagagaga agcuguucuc caaagaauuu gacaucgagc auauuauccc gcaggcucgg     2460

cuguucgaug acagcuucuc caacaagacg cuugaggccc gcucuguuaa uauugaaaaa     2520

ggcaacaaga ccgccuacga uuuugucaaa gagaaguucg gggaaagcgg agcugacaau     2580

ucccuggagc acuaucucaa caauaucgaa gaccuguuua agucuggcaa aaucagcaag     2640

acaaaauaca acaagcucaa aauggccgag caagauauuc cugacggguu caucgagaga     2700

gaucugcgga auacccagua uauugcuaag aaggcccuuu ccaugcugaa cgaaaucagc     2760

caccgcgugg uggcgacauc uggauccguc accgacaaac ucagagagga uuggcagcug     2820

auugacguga ugaaggaacu gaauugggag aaauacaagg cccuuggccu gguugaauau     2880

uuugaggauc gggacgggag acaaaucggc cgcaucaaag auuggacgaa gcggaacgac     2940

cauagacacc augcuaugga ugcccucaca guggcuuuca ccaaggacgu cuuuauucag     3000

uacuucaaua acaaaaaugc cagccuggau cccaacgcga acgagcacgc caucaagaau     3060

aaauauuuuc agaacggacg cgcuauugcc ccuaugcccc uccgggaguu cagagcugaa     3120

gccaagaaac aucuggaaaa uacacuuauc ucuauuaagg ccaagaacaa agugaucacc     3180

ggcaauauua acaagacccg caaaaaggga ggaguuaaua aaaacaugca gcaaacaccc     3240

cggggccagc ugcaccucga gacgaucuac ggguccggaa agcaguaccu gaccaaagaa     3300

gagaagguga augcuagcuu ugacaugaga aagaucggca cagucucuaa aagcgcguau     3360

cgcgacgccc ugcuuaagcg gcuguacgag aacgauaaug acccuaaaaa ggcuuucgcc     3420

gggaaaaacu cccucgauaa gcagccgauu uggcuggaca aggaacaaau gagaaaagug     3480

cccgagaagg uuaaaaucgu gacccucgaa gccauuuaua caauccggaa ggagauuagc     3540

ccugaucuga aagucgacaa ggugaucgau gugggcgucc gcaagauccu uauugacaga     3600

cugaaugagu acggaaacga ugcuaaaaag gccuuuucua aucuggacaa aaaccccauc     3660

uggcucaaua aggaaaaagg cauuuccauc aagcggguua ccauuagcgg gaucuccaac     3720

gcgcagucuc ugcacgugaa gaaagauaag gacggaaaac cuauccuuga cgagaacggc     3780

cgcaauauuc ccguggauuu cgucaacacg gggaauaacc aucacguggc uguuuauuac     3840

agaccgguga ucgacaagcg gggacagcug gucguggaug aagccggcaa uccuaaauau     3900

gagcucgaag agguuguggu cagcuucuuu gaggccguga caagagcuaa ccuggggcuc     3960

cccauuaucg acaaggauua caaaaccacc gaaggcuggc aguuccuguu uuccaugaag     4020

caaaaugagu acuucguuuu uccuaacgaa aagacaggau ucaaucccaa agagauugac     4080

cuucuggaug uggaaaacua uggccugauc ucuccuaauc ucuuucgcgu ccagaaguuc     4140

agccugaaaa acuacguguu ucggcaucac cuugagacca caauuaagga cacguccucu     4200

auccugagag ggaucaccug gauugauuuc cgcagcucca aaggacucga cacaaucgug     4260

aagguccggg uuaaucacau uggccagauc gugagcgugg gggaguauug a              4311


<210>  4525
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4525
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gaggcugaaa acaaagauga gcgguccucu aucgucaagc ugggcguucg cgugaauccc      120

cucacagugg acgaacugac gaacuucgag aaaggaaaga gcauuaccac aaaugccgau      180

agaacccuua aacggggaau gcgcagaaac cugcagcggu acaagcuccg cagagaaaca      240

cugaccgagg uccugaagga gcacaaacuc aucacagaag acaccauucu guccgagaau      300

ggcaaccgga cgaccuuuga aacauaucgc cuuagagcga aggcugugac cgaggagauc      360

ucucuggaag aguucgcccg gguucuccug augaucaaua aaaagcgcgg guacaaaagc      420

uccagaaagg ccaagggagu ggaagagggc acacuuauug auggcaugga caucgcucgg      480

gaacuguaua acaauaaccu caccccuggg gagcugugcc ugcaacuccu ggaugccggg      540

aaaaaguuuc uuccggacuu cuaccgcucu gaucugcaga augagcucga cagaauuugg      600

gaaaaacaga aggaguauua ccccgaaauc cugacggaug uccuuaaaga ggaacugcgg      660

ggcaagaaaa gagacgcugu gugggccauu ugugcgaagu auuuuguuug gaaggagaca      720

uacaccgagu ggaacaaaga aaaggggaaa acagagcagc aagaacgcga gcauaagcuc      780

gaaggaaucu acagcaaacg gaagagagau gaggccaagc gcgagaaucu gcaguggcgg      840

gugaacggcc ugaaagaaaa gcucucccug gagcagcuug ucauuguguu ccaggaaaug      900

aacacccaaa ucaauaacag cucuggguau cugggagcua ucuccgacag aagcaaagag      960

cucuacuuua auaagcagac cgugggccag uaucagaugg agaugcugga caaaaacccu     1020

aaugccucuc uucgcaacau ggucuucuac cggcaagauu aucuggacga auuuaauaug     1080

cucugggaga agcaggcugu uuaccacaag gaacugacag aggaacugaa aaaggagauu     1140

agagauauca uuaucuucua ucagcgccgg cucaaauccc aaaagggccu gauuggguuu     1200

ugcgaguucg aaagcagaca gaucgaggug gacaucgaug ggaaaaagaa gauuaaaacg     1260

gugggcaacc gcgucaucuc ucgguccagc ccccuuuuuc aggaguucaa gauuuggcag     1320

auccugaaua acauugaggu gaccguugug gggaaaaaga gaaaacggcg caagcucaaa     1380

gaaaauuacu ccgccaucuu cgaggagcug aacgacgcgg aacaacugga gcuuaaugga     1440

ucuagacggc ucugucagga agagaaggaa cugcuggccc aggagcucuu ugucagagau     1500

aagaugacaa aaagcgaggu gcugaagcug cuguucgaca acccucagga acucgaucug     1560

aauuuuaaaa ccaucgacgg caacaagaca ggguacgcuc uguuccaagc cuauuccaaa     1620

augauugaga ugagcggaca ugaacccguu gauuuuaaga agccugugga gaaagucgug     1680

gaguacauca aggcugucuu cgaccuucuc aacuggaaua ccgacauucu gggcuuuaac     1740

ucuaaugaag aacuggauaa ccaguccuau uacaaacucu ggcaccuucu uuauagcuuc     1800

gaaggcgaca auacgccgac agggaacgga cgccugaucc agaagaugac cgagcucuac     1860

ggcuuugaaa aagaguaugc caccauucug gccaaugugu cuuuccagga ugacuacggg     1920

agccuguccg cuaaggcgau ccauaagauu cuuccccacc ucaaagaggg aaaccgguac     1980

gauguugccu gcguguaugc uggcuacaga cacucugaaa gcucccugac acgcgaggaa     2040

aucgccaaua agguccugaa agacagacuc augcugcuuc cuaagaacag ccugcauaau     2100

cccgugguug agaaaauccu caaccaaaug gugaauguca uuaacgugau cauugauauc     2160

uaugggaagc cugacgaaau ucggguggag cuggcccgcg agcugaagaa aaaugcuaag     2220

gaaagagagg aacuuaccaa aucuaucgcc cagacaacga aggcgaacga ggaauacaaa     2280

acccuccugc agacagaguu uggccugacc aacguuuccc ggacagauau ccuccgcuau     2340

aagcuguaca aagagcuuga aagcugugga uauaagaccc uguacucuaa uacguacauu     2400

uccagagaga aacucuucag caaggaauuu gacaucgagc acauuauccc ccaggcucgg     2460

cuguucgaug acuccuucuc uaacaagacc cuggaggccc gcagcgucaa uauugaaaaa     2520

ggcaacaaga cagccuauga uuuugugaaa gagaaguucg gggaauccgg agcugacaau     2580

ucucuugagc auuaccugaa caauaucgaa gaccucuuua agagcggcaa aaucuccaag     2640

accaaauaua acaagcugaa aauggccgag caagauauuc cggacggguu caucgagaga     2700

gaucuccgga auacacagua cauugcuaag aaggcccuga gcaugcuuaa cgaaaucucu     2760

caccgcgugg ucgcgaccuc cggaagcgug acggacaaac ugagagagga uuggcagcuc     2820

auugacguua ugaaggaacu gaauugggag aaauauaagg cccugggccu uguggaauac     2880

uuugaggauc gggacgggag acaaaucggc cgcaucaaag auuggacaaa gcggaacgac     2940

cacagacauc acgcuaugga ugcccugacc gucgcuuuca caaaggacgu guuuauucag     3000

uauuucaaua acaaaaaugc cucucucgau ccuaacgcga acgagcaugc caucaagaau     3060

aaauacuuuc agaacggacg cgcuauugcc cccaugccuc ugcgggaguu cagagcugaa     3120

gccaagaaac accucgaaaa uacccugauc uccauuaagg ccaagaacaa aguuaucacc     3180

ggcaauauua acaagacacg caaaaaggga ggagugaaua aaaacaugca gcaaacgccc     3240

cggggccagc uucaucugga gaccaucuac gggagcggaa agcaguaucu cacaaaagaa     3300

gagaagguca augcuucuuu ugacaugaga aagaucgaga ccgugagcaa auccgcguac     3360

cgcgacgccc ugcugaagcg gcuuuaugaa aacgauaaug accccaaaaa ggcuuucgcc     3420

ggcaaaaaca gccuggauaa gcagccuauu uggcucgaca aggagcaaau gagaaaaguu     3480

ccggaaaagg ugaaaaucgu cacacuggag gccauuuaca ccauccggaa ggagauuucu     3540

cccgaucuca aaguggacaa ggugaucgau gucgggguuc gcaagauccu gauugacaga     3600

cuuaaugaau auggcaacga ugcuaaaaag gccuuuucca aucuggacaa aaacccuauc     3660

uggcugaaua aggagaaagg aauuagcauc aagcggguga cgauuuccgg caucucuaac     3720

gcgcagagcc uccacgugaa gaaagauaag gacgggaaac ccauccugga cgaaaacgga     3780

cgcaauauuc cugucgauuu cgugaacaca ggcaauaacc accauguugc uguguacuau     3840

agacccguca ucgacaagcg ggggcagcuu gugguugaug aggccggaaa uccgaaauac     3900

gaacuggagg agguggucgu guccuucuuu gaagccguua ccagagcuaa ccucggccug     3960

ccuauuaucg acaaggauua caaaaccaca gagggguggc aguuccucuu uucuaugaag     4020

caaaaugaau auuucguguu ucccaacgag aagauuggcu ucaauccuaa agaaaucgac     4080

cugcuugaug ucgagaacua cggacugauu agccccaauc uguuucgcgu gcagaaguuc     4140

ucccucaaaa acuauguguu ucggcaccau cuggagacca caaucaagga cacgucuagc     4200

auccuuagag gcauuaccug gaucgauuuc cgcuccagca aagggcugga cacaauuguc     4260

aagguucggg ugaaucacau cggacagauu gugucugucg gcgaauacug a              4311


<210>  4526
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4526
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuguggau       60

gaggcugaaa auaaagacga gcgguccucu aucgucaagc ugggcguucg cgugaacccc      120

cucacagugg augaacugac gaauuucgag aaaggaaaga gcauuaccac aaacgccgac      180

agaacccuua aacggggaau gcgcagaaau cugcagcggu acaagcuccg cagagaaaca      240

cugauggagg uccugaagga gcacaaacuc aucaccgaag auacaauucu guccgagaac      300

ggcaaucgga ccacguuuga aaccuaucgc cuuagagcga aggcugccac agagaaaauc      360

ucucuggagg aguucgcccg ggugcuccug augaucaaca agaaacgcgg guacaagagc      420

ugcagaaagg cuaaaggagc cgaagaaggc acccuuauug acggcaugga uaucgcucgg      480

gagcuguaua auaacaaucu cacaccuggg gaacuguguc ugcaacuccu ggacgccgga      540

aagaaauacc uuccggauuu uuaucgcucc gaccugcaga acgagcucga uagaauuugg      600

gagaagcaga aagaauacua ucccgagauc cugaccgaag uucuuaagga ggaacugcgg      660

ggcaaaaaga gagacgcggu gugggccauu ugcgcuaagu cuuucgucug ggaggaguac      720

uacacggaau ggaauaaaga gaaggggaaa acagaacagc aagagcgcga acauaagcuc      780

gagggaaucu auagcaaacg gaagagagau gaggccaagc gcgaaaaccu gcaguggcgg      840

gugaauggcc ugaaagagaa gcucucccug gaacagcuug uuauugugcu gcaggagaug      900

aacauccaaa ucaacaauag cucuggguac cucggagcua uuuccgacag aagcaaagag      960

cuguauuuua acaagcagac cgucggccag uaccagaugg aaaugcuuga caaaaauccu     1020

aacgccucuc ugcgcaauau gguguucuau cggcaagauu accucgacga guuuaacaca     1080

cugugggaaa agcaggcgga guaucacaag gaacugaccg gcgagcucaa aaaggagauc     1140

agagauauug ugaucuucua ccagcgccgg cugaaauccc aaaaggggcu ugucggauuu     1200

ugugaguucg aaagcagaca gauugaaguu gagaucgacg gcaaaaagaa gaucaaaacc     1260

guggggaauc gcgugauucc ccggucuucc ccucuguuuc aggaguucaa gaucuggcag     1320

auucucaaca auaucgaagu cacagugguu gggaaaaagg gcaaaagacg gaagcaaaaa     1380

gauaacagcc ccacgcuguu ugacgggcug gaugacaccg agcagcuuga acucaaugga     1440

ucccgcuacc uguaucagga ggaaaaggag cugcucgccc aggaacuguu cgugagagau     1500

aagaugacaa aaucugacgu ccuuaagcuc cuguucgaua acccucaaga gcuggaccug     1560

aauuuuaaaa ccauugacgg caacaagaca ggcuacaccc uuuuccaggc uuauagcaaa     1620

augaucgaga uguccgggca ugaaccggug gauuuuaaga agacgguuga gaaagugguc     1680

gaacacguga aggccgucuu cgaccuccug aauuggaaca cagauauccu gggauuuaac     1740

agcaaugagg agcucgacaa ccagucuuac uauaaacugu ggcaucuucu guacuccuuc     1800

gaaggcgaua auacccccac cgggaacgcu cggcucgugc agaagauuac agagcuguau     1860

ggauuugaaa aagaguacgc caucauucug gccaauguua ccuuccaaga cgauuacggc     1920

agccuuucug cuaaggcgau ccacaagauu cucccucacc ugaaagaagg gaacagauau     1980

gacguggccu gcgcuuacgc cggcuauaag cauagcgagu ccucucugac aaaagaggaa     2040

aucgccaaua aggcucucaa agaucgccug acgcuucugc ccaagaacaa ucuccggaac     2100

ccugucgugg agaagauucu gaaucagaug guuaacguga ucaaugccau cauugacacc     2160

uacggaaaac ccgaugaaau cagagucgag cuggcgcgcg aacuuaagaa aaacgcuaag     2220

gagagagagg aacucacaaa auuuauugcc gagaccacac ggaagcacga agaguucaaa     2280

auccugcugc agagcgaauu uggccucacc aauguguccc gcacggacau ucugagauau     2340

aaacuuuaca aggagcugga gagcuguggg uauaaaaccc ucuacucuaa cacauacauc     2400

ccgcgggaaa agcuguucuc caaagaguuu gauaucgaac auauuauccc ucaggcccgc     2460

cuguucgacg acagcuucuc uaacaagacc cuugaggcua gauccauuaa ucuggagaaa     2520

ggaaacaaga cagccuauga uuuugugaag gaaaaauucg gcgagagcgg ggcugacaau     2580

ucccucgauc acuaccugaa caauaucgaa gaccucuuua agucuggaaa aauuagcaag     2640

accaaauaua acaagcugaa gauggccgag caagauaucc ccgacggcuu caucaaucgg     2700

gaucuucgca acacgcagua cauugcgaaa aaggcccugu ccaugcucaa ugaaaucucu     2760

agacggguug ucgcuacaag cgggaccauu acagacaaac ugcgccagga uuggcaacug     2820

guggacguga ugaaggagcu uaacugggag aaauauaagg cccugggccu cgucgaauac     2880

uuugaggaua aggacggaag acagaucggc cggauuaaag auuggaccaa gagaaaugac     2940

caccgccauc acgcuaugga cgcccugacc guggcguuca caaaagauau cuuuauccag     3000

uauuucaaca auaagaacgc cucccucaau agcaacacga acgaauacgc uauuaaaaau     3060

aaguacuuuc agaacgggcg ggccaucgcu ccuauucccc ugagagaguu ccgcguugaa     3120

gccaagaaac aucuugagaa uacccugauc ucuauuaagg ccaaaaacaa gguggucaca     3180

ggaaauauca acaaaaccaa gaaacggggc ggggugaaua aguaucucca acagacaccc     3240

agaggacagc ugcaccugga gaccauuuac ggcuccggga agcaguaucu uacgaaagaa     3300

gagaagguua acgcuagcuu ugacaugaaa aagaucgcga cagugucuaa auccgccuac     3360

cgcgaugucc ugcucaagcg gcugcaugaa aaugacaacg auccuaagaa ggcuuucacc     3420

ggcaagaaua gccucgacaa acaaccgauc uggcuggaua aggagcagac cagaaaagug     3480

cccgaaaagg uuaagacagu gacccuugag acaauuuaua cgguccgcaa agagaucucu     3540

ccugaccuga agguggauaa agugauugac gccggagucc ggaagauccu gaccggcaga     3600

cucaacgaau acgggaauga ugccaaaaag gcuuuuagca accuggacga gaaucccauu     3660

uggcuuaaca aggaaaaagg aaucuccauc aagcggguua caauuagcgg caucucuaau     3720

gcccaguccc ugcacgugaa aaaggacaaa gaugggaagc cuauucucga cgaggauagc     3780

cgcaacaucc ccguggacuu cgucaacacc ggaaauaacc accauguggc gguuuauaga     3840

cggccgguga uugauaaacg cggccagcug gucaucgacg aggcugggaa uccuaaguac     3900

gaacucgagg aagugguugu guccuucuuu gaggccguca caagagccaa ccugggccaa     3960

cccaucauug auaaggacua caaaaccucu gaaggauggc aguuccuuuu uagcaugaag     4020

cagaaugagu auuucguguu uccuaacgag aaaacgggcu ucaaucccaa ggaaaucgau     4080

cugcuggacg cugagaacua cgggguuauu uccccuaauc ucuuucgggu gcaaaaauuc     4140

gcccacaaga acuaugucuu uagacaucac cuggaaacca caaucaagga uaccucuagc     4200

auucuuaaag gaaucacaug gauugacuuc cgcuccucua agggccugga ugcgaucgug     4260

aaagugcggg ucaaucacau cgggcagauu guuagcgugg gagaguacug a              4311


<210>  4527
<211>  4311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4527
augaaguaca ucaaauauau ucugggccuc gacaucggga gcaacuccau uggaugggcc       60

cugauccagc uugaucugcu ccaaaagacc aaaagaauua ucgcugccgg cucucggauc      120

auucccaugg accagaaggu gcugagcgau uucgggaaag gaaucacaau uuccccuacg      180

gcgcagcgca ccucuuacag aaaugcucgg cacugccgcg agagacaucu ucugcggcgc      240

gaaagacucc accggguccu gcauauccug ggcuuucucc cgacacacua ugacaagcag      300

auugauuuca gcaagcgcua cgggaaauuu guugacaaca ccgagcccaa gaucgccuau      360

aaugaaggaa aauuccuguu uauggauucc uuccaagaga ugcuuucuga cuuuaagagc      420

caucagccug aucuguucua cacaaagaag aacggcgaag aguccaagau ucccuaugac      480

uggaccaucu acuaccucag aaagaaggcc cugagccaga aaaucucuaa ggaggaacuu      540

gcuuggauua uccugaauuu uaaccagaaa cggggcuauu accaacuccg cgaggaagag      600

gggaauaagg agaacgauuu cgaaauuauc uccagcaaaa uuaucucugu gaucaagaag      660

gacaaagaua agaaauauaa uaaguacugg uaugacauua uccuggagaa cggacuggug      720

uaccaggccg cuuuuuauaa ugacuucguc uacaacugga uuaaucagga uagagaauuu      780

aucauugagc ggacaucccu caaaaacggc gccgaaaagg ugaagcugag cuaccuuccu      840

accuucgacg agaucgauaa aauggacaag aaagagcagg auaaguauua caaaaagauc      900

aagauuaaaa cggaaaauuc uaucgggaac aagaccguug gauguuauau uuacgaccac      960

auccugcaaa accccaauga gaaaauuaag ggcaaacucg ugcgcacaau cgaaagaaag     1020

uauuacaagg aggaacugaa agcgauucuu gagaagcaga ucgcccugca gccugaacuc     1080

uucaccgauc aacugcuggc ugacugcauc cgggaacucu auuccaaaaa cgaggcccag     1140

cagagaaauc uggcugcccg cgauuuuguc caccuucugg uggaagacau uaucuucuac     1200

cagcggccgc ucagacgcca aaagagcaca cuggcgaacu guucccuuga gucucggagc     1260

uacauugaua aagacuccca uaccagaaag gaagcucccc ugaaaguuug cccuaagagc     1320

aaucccuauu accaggaguu ucgcgugcuc caguggcugc agaaccugaa gaucuaugag     1380

auugacucug aucaagaagu cacgcaccag uucaucaaaa cacucgagga caagcagcag     1440

cuguuugagu uccuuauggc ccaaaaagag aucgauugug aggaacugcu caaguacuuu     1500

cugaaucaga ccuauccuaa cgccaaagag aaggcucuga aguccgaacu uaaaaagugg     1560

aaagacacau accgguggaa uuauguguac gauauugggg aaaagagcuc uaaaaaguac     1620

cccaugaacg aaaccagaua ugagcucaag cgccaucugg agaaaguggc caaucugccg     1680

gacgauuucc ucagcuccga agucgaguac cugcuuuggc accugaucua uucuguuaag     1740

gacaaagugg ccuacgaaaa gggacucaaa aaguuugcuc agaagcacca ucuggauaaa     1800

gacagcuucg uggaguccuu uaagaaauuc aacccuuauc ccagcgaaua cggcucuuuu     1860

uccgagaagg cgauucggaa acugcuuccu cucaugagau ucggcgccca cugggauuuc     1920

aaucauaucg acaagaacac ccagaaacgc auugaggauc ugaucacagg ggucgaaaau     1980

gaggaaaucc ggacgauucu gagagagaag gcugaaaagu aucaacucga gaaagagacc     2040

gacuuucagg accugccccu uuggcuggcc caguacaucg uguacaaccg gcacgccgaa     2100

gcuagcucuc ucgagaagug gacauccguu aacgaucugg aaaccuaucu gaaugaguuc     2160

aaacaacaua gccuucgcaa ccccauugug gagcaggucg ugauggaaac acucagaguu     2220

gugcgggaca ucuggcagca guacggacaa ggccaggccu ccuuuuucaa ugagauucac     2280

aucgaacugg ggagagagcu gaagaaaacc gcgaaggaac gcgagaagcu cucuaaacag     2340

aaccagaaga augaggauau caaccugagc auuaaagaaa aacuuaaaga gcuggaaaag     2400

uauaauacgc gggcuuacuc caucacacac caagagaagu auaaacucug gcuggaacag     2460

aagcauauuu cuccuuacac cggacagauc auuagcccgg agaaccuguu uaccgacaau     2520

uaugagaucg aacacauuau cccccagucc agauucuuug augacagccu uucuaacaaa     2580

gucgugugcg aguccaucgu caauaaggcc ccuuacaaag aucgccaacu cggccuggag     2640

uucauuaaga accagggagg aagaaucgug aaggagcuga gcaaagaaaa uaagacaauu     2700

aaaaucuuua ccgaggagca guaccggaca uucauuaagg aacauuauuc uaacaauccc     2760

gagaaaacga agaagcuccu gcuugaagag aucccugaga aaaugguugc ccgccaaaug     2820

aacgacaccu guuacaucuc caaguuuauu agcgaaaucc ugucuaaaau ugugagaagc     2880

gaugagcagg acgaaggcgu ccacuccaag aacgugaucc ucugcacagg caaaauuacc     2940

agcauccugc ggcaggauug ggggcugaau gacaucuggg auaaccuuau ucugccccgc     3000

uucgagagaa ugaauaaccu ccugcagaag gaaaaauuua caaucuauuc uauugagaag     3060

caaaagaaaa ucccgguugu gccuauuaau gaccaguuca aguccuucca gaaaaagcgg     3120

aucgaccacc gccaucacgc uauggaugcc auuaucaucg cuuguaccac gagagagcau     3180

auuaacuaca ucaauaacca gcacgcccuc gaaaaagaga aggacaagaa agaaaagcaa     3240

gaggaacggg auaaacugcg cgagaaacuu ugcauuaaaa aguauaauaa cagaagcgag     3300

ggaaauuacu ccuggaucuu uaagaaaccc ugggacaccu ucacacagga aguccgggug     3360

gcgcuggagg auauuguggu uucuuuuaag cagaaccuca gagucaucaa uaaagccacc     3420

aacuauuacg aacgcugggu ggaggaaaau ggcaaguaca ucaaaaagau ggugaagcag     3480

gaggggacaa acugggcuau ucggaaaccu cugcaugagg aaaccaucag cggaaagauu     3540

uaucuggaca gagagaaaau cacgaagaac gauauucuua cagccacccg caaaacaguc     3600

gacuccucuu ucgaugacaa gcggaucaaa aagaucaccg auaccggcau ucaaaagauc     3660

cugcucaauu accugaaaua uaaggggagc cccgaaauug cuuuuucccc ugagggccuc     3720

gaggaacuga acaaaaaucu uucuaucuac aacgacggaa agccccacaa accgauuuau     3780

aaggugagaa ucuacgagaa gggcagcggg cgcuucgccc ugggagaaaa aggccggaag     3840

uauaaaaagu acguucaggg ggcguccaau cucuacuuug gaauuuauca gggcgccaaa     3900

cgcagcuucg cuacaauccc ucugaacgag gugaucgaaa gacagaagca agggcugccc     3960

ucuguccccg aguacaauga gaagggcgac cuucugcucu uuucccugag cccuaacgau     4020

cucguguaug uuccgauuga aggagagauc auugaaaaua ucgacuuccg gaaccugucu     4080

aaagagcaga aggaaagaau uuacaaugug aacgauuuuu ccagcacgug uuauuucacc     4140

cccaaucgga ucgccaaagc uaucuucccu aaggaggucg accuucagcg caaaggcgau     4200

aagcugucug gguccuacga cacaaagacc gccagccugg aggggauuca aaucaaagaa     4260

gugugcauua agcucaaagu ugauagacug ggcaacaucu ccaaggccug a              4311


<210>  4528
<211>  4314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4528
augaaguaca ucaaauauau ucugggccuc gacaucggga gcaacuccau uggaugggcc       60

cugauccagc uugaucugcu ccaaaagacc aaaagaauua ucgcuacagg cucucggauc      120

auucccaugg accagaaggu gcugagcgau uucgggaaag gaaucacgau uuccccuacc      180

gcccagcgca caucuuacag aaaugcgcgg cacugccgcg agagacaucu ucugcggcgc      240

gaaagacucc accggguccu gcauauccug ggcuuucucc cgacccacua ugacaagcag      300

auugauuuca gcaagcgcua cgggaaauuu guugacaaca cagagcccaa gaucgcuuau      360

aaugaaagaa aauuccuguu uauggauucc uuccaagaga ugcuugccga cuuuaagauu      420

caucagccug aucuguucua caccaaaaag aacggagaag agucuaagau ccccuaugac      480

uggacaaucu acuaccuccg gaaaaaggcc cugagccgca aaauuuccaa ggaggaacuu      540

gcuuggauca uucugaauuu uaaccagaaa agaggcuauu accagcuccg ggaggaagag      600

gaggaaaaua aggagaacga uuucgaaauc auuagcucua aaaucaucuc cgugauuaag      660

aaggacaaag auaagaaaua uaauaaguac ugguaugaca ucauucugga gaacggccug      720

guguaccaag ccgcuuuuua uaaugacuuc gucuacaacu ggaucaauca ggaucgcgaa      780

uuuauuaucg agagaaucag ccucaaaaac gggaccgaga aggugaagcu gucuuaccuu      840

ccuacguucg acgaaauuga uaaaauggac aagaaagagc aggauaagua uuacaaaaag      900

aucaagauua aaaccgaaaa uuccaucgga aacaagacag uuggcuguua uauuuacgac      960

cacauccugc agaaccccaa ugagaaaauu aaggggaaac ucgugcggac caucgaaaga     1020

aaguauuaca aggaggagcu gaaagccauc cuugaaaagc aagucgcgcu gcagccugaa     1080

cucuucacag aucagcuguu ugccgacugc auucgcgaac uguauagcaa aaacgaggcu     1140

caacagcgga aucucgccgc uagagauuuc gugcaccugu uuguugaaga caucauuuuc     1200

uaccagcgcu cucuucggag acagaagucc acccuggcca acuguagccu cgagucccgc     1260

ucuuacaucg auaaagacag ccauacgcgg aaggaggcgc cgcugaaagu gugccccaag     1320

uccaauccuu auuaccaaga auuuagaguc cuucaguggc ugcagaaccu caagauuuau     1380

gagaucgaca cagaucagga agugacccac gaguucauca aaacacugga ggacaagcaa     1440

caguuuuucg auuuucugau gaaucagaaa gaaauugacu gugaggaacu ccugaaguac     1500

uuccuuaacc agaccuaucc caaugcccgc gagaaagcuc ugaaguccga acucaagaaa     1560

uggaaggaua ccuaccggug gaacuaugug uacgacaucg gagagaaauc uuccaagaaa     1620

uacccuauga augagacaag auaugaacug aagcgccauc uggagaaggu cgccaaccuu     1680

cccgaugacu uucucagcuc ugaaguugag uaccugcugu ggcaccucau uuauagcgug     1740

aaagauaagg uggcuuacga aaaaggccug aagacguucg cccaaaaaca ccaucuugac     1800

aaggauuccu uugucgaguc uuucaagaaa uucaagccgu auccuagcga guacgggucc     1860

uuuagcgaaa aagccauccg gaagcugcuc ccccugauga gauucggauc uuauugggac     1920

uuuaaucaca uugauaaaaa cacccagaag cggaucgagg accugaucac aggcguggaa     1980

aaugaggaaa uucgcaccau ccuuagagag aaagcugaga aguaccagcu cgaaaaggag     2040

acagacuucc aggaucugcc ucuguggcuc gcgcaauaca uuguuuauaa ccggcaugcc     2100

gaagcuagca gccuggagaa auggaccucu gugaacgacc uugagacgua ccugaaugaa     2160

uuuaagcagu auucccucag aaaccccauc gucgaacagg ugguuaugga aacacugcgc     2220

gugguccggg auauuuggca acaguacggc caggggcagg ccagcuucuu ugacgagauc     2280

cacaucgaac ugggaagaga gcuuaaaaag accgccaaag agcgcgaaaa gcucuccaag     2340

caaaaucaga aaaacgagga uauuaaucug ucuaucaagg aaaaacugaa ggagcucgaa     2400

aaauauaaca ccagagcuua cagcauuaca caucaggaga aguauaagcu guggcuugag     2460

cagaaacaca ucucccccua caccggccaa auuaucucuc cugaaaaucu guucacagac     2520

aacuacgaga uugaacacau caucccgcag agccgguuuu ucgaugacuc ccucagcaau     2580

aagguggucu gcgagucuau ugugaacaaa gcccccuaua aggaucgcca gcuggggcug     2640

gaauuuauca aaaaucaggg aggcagaauu guuaaggagc uuuccacgga gaacaagacc     2700

aucaaaauuu ucacagagga gcaauaccgg accuuuauca aggaacauua uagcaauaac     2760

ccugagaaaa caaagaaacu ccugcuggag gaaauccccg agaagauggu ggcgcgccag     2820

augaaugaca ccuguuacau uucuaaguuc aucuccgaaa uucucagcaa aaucgucaga     2880

ucugaugagc aggacgaagg ggugcacagc aagaacguua uucugugcac gggaaaaauc     2940

accuccgcuc uuaagcaaga uuggggcgug aacgacgucu ggaaugaccu gauccucccu     3000

cgguucgagc gcaugaacga ucugacacag agcacccugu uuacaaccua uaaugagaaa     3060

uaccagaagu aucuucccac ggugccgaug gaauacucua acggcuucca gaaaaagaga     3120

auugaccauc ggcaccacgc cauggaugcc aucauuaucg cuugugccac acgcgagcau     3180

auuaauuaca ucaacaauca acacgcucug gaaaagggga aagacaagaa agagaagcag     3240

aaagaacaug auaagcucag agagaagcug ugcauuaaaa aggacaacaa aggauccgag     3300

gaaaauuaua gcuggaucuu uaagaaaccu ugggauaccu ucacacagga ggugcaggcc     3360

gcgcucgaag gcaucguugu cuccuuuaag caaaaccugc gggugauuaa uaaggccacc     3420

aacuacuaug agagaugggu ggaagagaau gggaaauaca ucaagaaaau ggucaagcag     3480

gagggaacca acugggcuau ucgcaaaccc cuucacgaag agacaaucuc uggcaagauu     3540

uaucuggacc gggaagagau cacgaagaau gauauccuca ccgccacaag aaaaaccgug     3600

gacagcuccu ucgaugagaa gcgcauuaaa aagaucacag acaccgggau ucagaaaauc     3660

cugcugaacu accuuaagua uaaaggcucu ccugaaauug cuuuuagccc cgagggacug     3720

gaggagcuca acaagaaucu guccaucuac aacgacggca agccucauaa acccaucuac     3780

aagguucgga uuuaugaaaa agggucugga agauucgccc ucggcgagaa ggggcgcaaa     3840

uacaagaagu augugcaggg agcgccgaau cuguacuuug gcaucuauca aggggccaac     3900

aaacggagcu ucgcuacgau uccucuuaau gaggucaucg aaagacagaa gcagggccgc     3960

cccuccauuc ccgaguacaa cgaaaaagga gauccucugc ucuuuagccu gucuccgaau     4020

gaccuggugu auguucccgu ggagggcgaa aucauugagg auaucgacuu ccagaaccuu     4080

uccaaggagc aaaaagaacg gaucuacaau gucaacgauu ucagcucuac auguuacuuu     4140

accccuaaua gaauugccaa ggcuaucuuc cccaaggagg uggaccugca gcggaaaggg     4200

gauaagcucc cuggauccua ugacaccaaa acagccagcc uggaaggcau ucagaucaag     4260

gagguuugca uuaaacucaa gaucgaucgc cuggggaaca ucucuaaggc cuga           4314


<210>  4529
<211>  4317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4529
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc ccuuaucaaa       60

cagaauuucg agaacaagua cggcgaaauu cuggggaugg gauccagaau cauucccaug      120

ucucaagaua uccucggcga guuugggaaa ggaaauagcg ugucccagac ggcugcccgg      180

accgacuauc gcgcgacaag acggcugcgc gaaagauucc uucugcggcg cgagagacuc      240

caccgggucc ugaacguucu gaauuuucuc ccugaacauu acgcuucuca gaucgauuuc      300

gacaagcgcu uuggcaaauu caagguggag accgagccga agcuggccug gaaaaacauu      360

gauggcaagu uuagcuuccu uuuucagaca uccuucaaug aaaugcugga ggacuuuaaa      420

gcccacgggc aagaucucaa ggugcccuau gacuggacca ucuacuaucu gagaaagaag      480

gcucuuucuc agaagauuga aaaagaggag cuggucugga uccuccugaa cuucaaucag      540

aagcggggau acuaucagcu gcgcggcgaa gaggaagagg aaaacccuaa uaaacucauu      600

gaguuuuaca gccugaagau cauugaugug cuugccgacg agccccaaaa agggaagucc      660

gauaucuggu acagccuggu ucucgaaaac ggauggaucu auagacgguc uuccaaaaca      720

agccugcuug acuggaagga uaagauucgc gacuucaucg ugaccacgga ccugaaugau      780

gacggcucug ucaaaaccga uaaggagggg aacgagaaaa gauccuuccg ggcuccugga      840

gaaaaugacu ggacacucgu gaagaccaaa acagaacagg agauugauag aagccgcaag      900

accguuggca acuacaucua ugagacgcug cugcagaacc ccaagcagaa aauuaagggc      960

aaacucgugc ggacaaucga aagaaaguuu uacaaagagg aacugaagcg cauccuugag     1020

aagcaaaaag aauuucaucg ggagcuggag ucuaaugacc ucuauaacga uugcauuaga     1080

gaacuguacc gcaauaacga ggcccaccag cuuacccugu ccaagaaaga cuuuguccau     1140

cucuucaugg aagauaucau uuucuaucag cggccucuca agagccaaaa auccucuauc     1200

agcaauugua cacuggaguu cagaaaguac aaggacgaga acgggauuga acacacccag     1260

uuucugaaag cgaucccgaa guccaauccc uacuaucagg aguuccgcau cuggcagugg     1320

auuuuuaacc ugaauaucua caaaaaggau aacgacgaca augugaccaa agaguuccuu     1380

aacacaacgc gggauuuuga aaaucuguuc gaauuucuca acaauagaaa ggagauugag     1440

caaaaaccuc ugcugaaguu ccuucucgaa cagaaggagu ucaaaggaaa gcugcugaac     1500

gccgaaaccg agaaauuucg cuggaacuau guggaagaca agaaauaccc cugcaaugag     1560

acacggacca ugaucagcga gagacucaag aaggucgaaa acauuucuga ugacuuccug     1620

acacgggaga uggaacagaa aaucuggcac auuaucuauu ccguuaauga uaagaccgag     1680

uacgaaaaag cucuuaagag cuuugccgag aaaaacaauc uggacgagaa cucucucuuc     1740

gaggcuuuua agaagcugcc ucccuucaaa agcgaauaug gcuccuuuuc ugaaaaggcc     1800

aucaaaaagc ugcuuccgcu caugcgccug gggaaauacu ggagcuacgc gaagauugau     1860

cuguauucca agaauagaau ccagaaaauu aucacgggag aguucgacga gaacauuaag     1920

gaucggguga gagaaaaagc cauccgccuc acagcugaga augacuuuca aggccugcag     1980

cuuuggcugg cccaguacau cguguauggg cggcauagcg aggcuucuau gauuggaaag     2040

uggaacuccg ccgaugaccu cgaagaguuc cugaaagaau uuaagcagca cagccugaga     2100

aauccuaucg ucgagcaagu gauuaccgaa acccuucgca ucguuaaaga uauuuggcuc     2160

aaguacggca acggcgccaa ggacuucuuu aaugagaucc auaucgaacu ggggagagag     2220

augaaacaga caaaggacga acgggcuaac gcgaccaaaa caauuacgga gaaugagaac     2280

accaaccugc gcaucaaggc ccuccuggcu gaaaugauga augaucacuc uguggagaac     2340

gucagacccu auuccccuau gcagcaggaa auucuuaaaa ucuacgagga cggcauucuc     2400

aagagcgaua ucgaaaucga cgaugacauu cugaagaucu ccaaaacagc ccaacccucu     2460

agcuccgauc ugaagcggua uaaacugugg cuugagcaga aguacaaauc ucccuacacc     2520

ggacagauua ucccucucaa uaagcuguuc acaccggagu augaaauuga gcacaucauu     2580

ccccaaagcc gcuacuucga cgauuccuuu agcaacaaga ucaucuguga aucugccgug     2640

aauaaacuga aggacaacua uauugggcuc ggauucauca aacaguuugg cgggaccauu     2700

aucgagugcg gcuucggaaa gagaguuaaa guguuugagg ucggcgaaua cgaggaguuc     2760

gugaagaagc auuaugcuaa uaaccggggg aaacgcaaua agcugcuucu ggaagaaauu     2820

ccugagaaaa ugaucgagag acagaugaac gauacgcggc acaucuccaa guacauuagc     2880

ggaauccucu cucauauugu ccgcguggaa gacggcaccg augagggggu uaauuccaaa     2940

aacgugaucc ccggaaaugg caagauuaca acccagcuga gacaagacug ggggcugaac     3000

gacgucugga augaacuuau ccugccucgg uuugagagaa ugaaccagcu cacaaacagc     3060

accgauuuca cgucuuggaa ugaaaaccac cagaaguauc ugcccacagu gccgguugag     3120

uuuagcaaag gcuucuccaa gaaacgcauc gaccaucggc accacgcccu cgaugcgcug     3180

gugauugcuu gugccaccaa ggaccauguc aaucuucuga acaaucagag cgccaaaucu     3240

gcuacaaaga gauacgaucu caaaaagaag cugaugaaau uugagaagac cguguacaaa     3300

gacccucaaa ccgaaaagcg caucgagcgg gaagugccca aacacuuccu gaagccuugg     3360

gagacauuca cgguugaugc caagaacaga cuugaaacca uuaucgucuc cuuuaaacag     3420

aaucugcgcg ugauuaacaa ggcuacaaau uauuacgaga aauaugugga gaaggacgga     3480

gucaugauga aagaacgggu ggagcagacc ggcacaaacu gggccaucag aaagcccaug     3540

cauaaggaaa ccguuagcgg gaaaauugau cucccuuggg ugaagguccc caaaggaaag     3600

auccugacgg cgacacgcaa aucccucgac accucuuucg aucugaaggc caucgcuagc     3660

auuaccgaca caggcaucca gaagauucuu aaaaauuacc uggaguuuaa ggaguccccg     3720

gaacucgccu ucucuccuga ggggaucgaa gacaugaaca aaaauauuga gaaguauaac     3780

ggaggccggc cccaccaacc caucagcaaa gugagaguuu uugaacuggg guccaaguuc     3840

caggugggcc agaccggaaa uaaaaaggau aaguacgucg aggcugccaa aggcacaaac     3900

cuguuuuucg cgaucuauga ggacaagaaa gggaagcggu cuuacgaaac gauuccucuu     3960

aaugagguga ucgaacgcca aaaacaggga cugagcguug uggaucucaa gaacgucaac     4020

gacuuuuucc ugugcccgaa ugaucucgug uacauucccu ccggcgacga guuugaaaac     4080

agagggagca ucgagauuga gaauaucacc aaggaaaaau cugagcgguu cuauaaggug     4140

gucuccuuua gcggaucuca gaucuucuuc guucgccacg auauugccgu guccaucgug     4200

aacaaaggcg aauuuagcac acugaauaag auggagagag cuauugacgg gucuaugguc     4260

aaagaaaucu guauuaaacu uaagaucgau cggcugggcg acgugcugaa agccuga        4317


<210>  4530
<211>  4317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4530
auggugaaga aaggcuacac caucgggcug gacauuggaa caacgagcgu cggcugggcc       60

guuaccgaug acgauuucga gcucgugaac aagaaaaugu ccaucaagga caaaucuggg      120

agcacaauua agagagugcg gaagaaucug uggggagucc gccuuuuuga ugaagcuaaa      180

accgccgaga acacaagacg gacccgcggc gugagacggc gcaucagacg gcgcaaggaa      240

agacugaauu aucuccaggg gauuuucucc ucugagaugg cgaaaaugga cgaaaacuuu      300

uucaugcggc uggaggagag cuuuuucaaa cuuuccgaua agucugaaca aaauaaguuu      360

cccuacgcuc uguucaagaa cgagaucgaa gagaaagccu auuaccgcca guuuccuaca      420

aucuaucacc ucagaaagca ucugauggag cacgaagaga gcgaccugcg gaaaauuuac      480

cucggacugc aucacauccu uaaguaucgc ggccauuucg uucaggaagg ccagaaaaug      540

gauguggcca auauugacau ccaagggaac cugaaggagc ucuuuaaugc ucugaacgaa      600

aauuucucca acaaagagga ugacuuugag caggaaagaa ugaaggaugg agucaagcgg      660

gccaaagugc uuuucgacac cgagaagcug gcugccguug aagagauucu caaagaaaag      720

aucagcaaau cuuggaaggu gaauaagauu uccaaaggca uccuggcgga gaagaaagac      780

cugccgaucu ucaaggccau ugucgggaac aaaaucgauc ucgcuggaau uuuugacugc      840

cccgauuacg ccgagaaggu gaaugaaagc aucaaggagc ugaaagacaa gguguacuuc      900

aacaaagaag auuuugagga aacguucgag aaacuuucuc agcugcucaa ggaggaagaa      960

cuggaaauuc uuauccuggc uagaaagugu uaugaggccg uccuccuggc ggacaucaug     1020

cagggcgagc aaucccugag cgaugccaug guugcuaagu acaacgacca caaaaaggau     1080

cucgugauuu uuaaaaaguu cgugaucgac uauuuuucug aagaugucua cuucgaccug     1140

uuuaaaaaua cccagcuugc cgggaacuau cacuccuacg ugaagggaga gcgcaaaggc     1200

aauaaguuca accaugcuag ccuggacgau uuuuauaagu ucguuaaaaa ggaacucgac     1260

aaaaaggccg aggaacuguc cgagcacccu guguacaaac agauucuuca gaagaucgag     1320

cuggaaacau uucuccccaa gcaacggaug uucaaaaaug gcgccauucc uuaccagguc     1380

caucugcacg agcugagagc uaucaucaag aaacaggaaa agcgcuuccc cuuucucgcg     1440

gagaaaauua ucauuaagga uaaccaaaag gacaucucug aauauaaaau ugagacccug     1500

augaaguucc ggaucccuua cuaugugggg ccgcuuauug aggccagaga aggagagcug     1560

ggcaaaaagc gcacaaauua cagccgguuu gcuuggaugc agaaaucccc cgaaagccag     1620

aacaucggga ucaccccuug gaauuuccac ucuguugugg auaaggacgc cuccgccagc     1680

gaguuuauug aaagaaugac gggauucugc ucuuauuuuc ccgaggagcc uguccucccc     1740

aagaacagcc ugaucuacca gcaauucaca guguuucagg aacuguccau ugucggcuau     1800

uacgaugagc agaaucgcaa acauuaccuu gacggggaaa cccggagacg caucuucgag     1860

gaucucuuua agaaaaaccg gacagugucu gcuggacugc ugagaaagug gcucguuugu     1920

aaucagcgga ccagccaaga ggaaaaagag auucgccugu ucggccuuga ccugaagaaa     1980

aacggccucg gguccggaaa uuuuaacacc aaguauacaa cguaccugga ucugaaggac     2040

gccggcauca ccgaaaaaca gcuuaagcag cacaaagauu ucuucgagaa gaucgugaaa     2100

uaucagacaa uuuuugaaga caagaagauc cucaaaagac ggauuauccu gcugaacgag     2160

gagcaaccgc uccugagcga ggagcagauu aagaaucuug cgaaaaagca uuacaccggg     2220

uggggaaacc ugucucucaa acugaucgac ggcauccagg auaaggggaa gacaauucug     2280

gacuaucuug aaacccagga uuacaaucaa aaccucaugc agcugaugaa ugacgagaac     2340

aaauccuucg aaaugcagau cgagcaagcu agagccggca agguccaguu uggaaaaauu     2400

gauuauagcu ucguggagca gcuggccggc ucuccugcua ucaagcgcgg gauuuggcag     2460

ucccuccaaa ucguugagga acugguggcc uuucuuggaa aagaaaauau caagaagauu     2520

gucaucgaga uggcucggga aaaccagagc uccaaaagaa cgaagucucg ccagaaacag     2580

auugaggccc uguaccaaaa ggugacaggc gaccucaaaa augagcagga aaagaucgau     2640

gugaugcaga acuucgacaa ugagagaguu uuucuguacu aucugcagaa cgggaagugc     2700

augcacagcg gagaaaaacu ugagcucucc caacugucug aguacgaaau ugaucauauc     2760

auuccccaga ccuauaucaa ggacgauagc uucgacaaua aaguccuggu gacccggaac     2820

gcgaaucagc aaaagggcgg ggaugugccu uccuacgacg ucaucgacaa aaugaaaagc     2880

uauugggaga agcucaagga aaacaaccug auuucugaga agaaauuugc caaucuuaca     2940

aagggcaaau ucgaugacca gcagcugucc ggauuuauca accgccagcu cguggaaacc     3000

agacaaauua caaagaaugu ugcuaucauu cuggauaaaa ucuacaacga cgaggccaag     3060

aaugagaagg aaguggcuau cauuacgcug aaaagcgccc uuaccucuca guaucggcag     3120

ggcaucauuu acguccccaa cgaugacuuc gaugagcuga agcucguguc caaaacaaau     3180

agccgccuga aggaaguuaa acuccacgac ggguucuaca aggugagaga gaucaacgau     3240

uaucaccaug cgcacgacgc cuaccugaau aacgucgugg cuaccuaucu uuacgaagcc     3300

augcccgauc ugcggccucu cuuuguuuau ggagagcauu cucgcgagau ucacaagaga     3360

uuccugggca aauacgcuac aacccggaag gggcaguuua aacaacugcu uguggacaug     3420

caggacgaau auuggaugcg caaggauaaa gagggaaauu ucaucgacgg cgaaauccug     3480

uggagacggg augagguccu ccagauugug aagaaggugc uggacuacag aaacauccag     3540

auuacgaaaa agcucgaaau gauggucacc gggggcgagg agaaauccuu uuaccaagau     3600

agccgcuguc ggaaggacaa aacagccauc ccguauaaga ccaauuggga ucccagcaaa     3660

uacggaggcu ucaagucucc uagaacagcc uaugcuauuc ccguuaucaa gaaaaacaag     3720

ccuaaacugg aacccgugcu ucuggugaau cgcuccaagg ucgagaccga cgggcaguuu     3780

gauugcgaaa aauucaagga gcucaacccg acgcugaaga uucugggaca gccuaucugg     3840

aaauaccaga gcuuugagau guucgacggc acaaagcggu auaucgcguc cgccucugaa     3900

auucacaaag ggaaccaaau caaguuuagc caggagcaua ugaaauucau uuccuguugc     3960

cagaaguacg augaagugaa gaauaaacuu gcuuuugacu ucguuaacga gucuaagcac     4020

cuguuccaag aaauccucac cgccauuaaa gauaauaucc auagauaucg ccugaucgcc     4080

gagggaauug aggugcaccg gaaguuuagc aacaugcucg aaaauaucga gaaacugucc     4140

acacaggaac uugucgacaa ccugcuggac gcuauuaaua ucaccucugu gggcaccaca     4200

aacaaugccu ucgggagagu ucgcuacaag ggcagcuacc ucaacgagau uugguccgcg     4260

acgcugaucc aucagagcgu gaccggacuu uaugaaacac gggagaagcu gaucuga        4317


<210>  4531
<211>  4323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4531
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc ccuuguggag       60

caggauuucg aaaauaaaca aggccugauc cucgggaugg gauccagaau uauccccaug      120

gacgcuggca uuaucgggaa guuugccgag ggaucuagca ucucccagac ggcggaacgg      180

accggcuacc gcucuauuag acggcugcgc gagagacacc uucugcggcg cgaaagacuc      240

caucgggucc ugaacauccu gaaauuccuc ccugagcacu augcugccga gauugauuuu      300

gaaaagcgcu ucggccaguu ucuggccgag acagaaccga aacuugcuug gaaaaagagc      360

gccgagggga aauucgaguu ucuguuccag agauccuuug acgaaauggu ugcugauuuc      420

aaggccaaug gacaagacau caaaauuccc uacgauugga ccaucuauua ccuccggaag      480

aaagcgcuga cacagaagau uucuccucag gagcuugccu ggaucauccu gaacuuuaau      540

cagaagcgcg gcuauuacca acucagaggg gaagaggaag aggagaaccc caauaaaaag      600

guggaguucu auagccugaa aauuguggac gucguggcug augaacagcc uaacaagaaa      660

ggagacaccu gguacucccu gcaucucgaa aauggcuggg uuuaccggcg cagcucuaag      720

aucccccugu augauuggaa agacaaggug agagauuuua uugucacaac cgacaucaac      780

gaggacgggu ccgaaaagcu ggauaaagac ggagagguga agcggagcuu cagagccccu      840

aaagaggaug acuggacgcu gauuaagaaa aagaccgaac aggagaucga acaguucaau      900

caaacaguug gcucuuacau cuaugaggcu cuccuggaua acccgaccca gaagauucgc      960

ggcaaacuug ugcggacaau cgaaagaaag uuuuacaaau ccgagcugaa gaaaauucuc     1020

gagaagcaga ucgaacugca acccgagcug uucaccgaag accucuauaa ugccugcgcg     1080

cgcgagcugu accggagcaa cgucgcccac cagcagcagc uuucuuccag agauuuugug     1140

uaucuguucc ucaaugacau uaucuuuuac caacgcccuc ugcggagcca gaagucccuu     1200

aucucuaacu guagccugga guucagaacg auuaaagaua aggacgggaa cggacagauc     1260

auuuaccuca aagcuauccc caaguccaau ccuuauuacc aggaauuucg cguguggcaa     1320

uggcuguaua accugaaaau uuucacaaag gaggaugaca aggacgucac caaucaguuu     1380

aucaacaaag uugaagaucu cgaggcccug uucgaauuuc uuaugaauca gaaggaggug     1440

aaccauacag acauucugac cuaccucauc gagcccaucg ugaaaagcaa guauccgaau     1500

gcuaaaggca aggccuucaa agaugaacug aagaaggagc ugaaaaagua ccgguggaac     1560

uaugucuacg acgccgauaa agacgaaucu aaguccuacc cuaugaauga gaccagauau     1620

gaacuucgca aacggcucga uaagguggag aacguucccg cugacuuuau gacaagaaag     1680

auugagcagc accuguggca caucauuuac agcgugacgg auaaaaucga guucgaaaag     1740

gcgcugaaau cuuucgccaa uaaguaugac cuccaucaag agagcuuugu cgaaaacuuc     1800

aaacgguuua agccuuucga uuccgacuac gggaccuauu cugaaaaggc uauuaaaaag     1860

cugcuucccc ugaugcgcgu gggaaaauac uggagcuggg agggcaucga uaauaacaca     1920

aagaaaagaa ucgggaagau uaucaccgga gaguaugacg aaacaauuaa gaaucggguu     1980

agagagaaag ccaucgaccu caccgaagag aaccacuuuc agggccugca gcuguggcuu     2040

gcccaguaca uuguguacga ucgccauucc gaggcugaca ucgccggcaa guggaacagc     2100

gucgcggauc ucgaacaaua ucugucugag uucaaacagc acucccugcg gaaucccauc     2160

guggaacagg ucauuacgga gacacucaga gugguucgcg acaucuggaa gcaauacggg     2220

aaaggagcua aggauuauuu ugacgaaauu cacaucgagc ugggcagaga gaugaaaaac     2280

accgccgagg aacggaagcg ccuuagcucu auuaucuccg aaaaugagac cacaaaccug     2340

agaauccggg ccauucucga ggaacugaug uacgaugaga auauggaaaa cgugcgcccu     2400

uauagcccga ugcagcagga ggcucugaag aucuacgaag acgggguccu uuccucuagc     2460

guggagauuc ccgaagauau cgagaaaauu uccaagaaau cugagccuac caagagcgaa     2520

auccagagau auaaacucug gcuggagcaa aaguaccggu cccccuacac aggacgcccu     2580

auuccccuga gcaaacucuu cacgccggcc uaucagaucg aacauaucau uccucaggcu     2640

agauuuuucg acgauggcuu uucuaauaaa guuaucugcg aguccgaggu gaacgcccug     2700

aaggacaaaa ugcuugggcu ggaguucauu cagaauuacu auggccaaaa ggucaccacc     2760

agcuuuggag aagugacaau ccucacagaa gaugcguaca aagccuucgu gaaggagcac     2820

uaugacaaga accggucuaa acgcaauaag cugcugcuug aagagauucc cgagaaaaug     2880

aucgacagac agaugaacga uacccgguac aucuccaagu auauuagcaa aguucucucu     2940

aaucugguca gaggcgaaaa ggacgaugag ggggugaaca gcaagaauau cauuccugga     3000

aacggcaaaa ucuccgggac gcugaagcag gacuggggac ucaacgaugu guggaaugac     3060

cugauucuuc cccgcuucga acggaugaac cagcugacca auagcacaga uuuuaccgcu     3120

uggaacgaga aacaucaaaa guuccucccu acaauccccc uggaacuguc uaaaggcuuu     3180

cagaagaaga gaaucgacca ccgccaucac gcccuugaug cucugauuau cgccugugcg     3240

acccgggacc acgucaaucu ccugaacaau aaacaugcca aguccagaaa cgagcgcuac     3300

gaucuccagc acaaacugcg gcauacgaca ccguggcaag acaaggaggg gaaagugaga     3360

accaaguuca cagaauuuaa aaagccuugg gagaccuuca cccaggacac aaagaaagaa     3420

cuugagaaga uuguugugag cuuuaaacag aaucugcgcg ucaucaacaa ggcuacgaau     3480

uacuaugaaa agauugugga uggcaagaag guuauguuca aacaggaggg agugaacugg     3540

gccauccgga agcccaugca caaagagacc gucuccggca agauugaccu ccccagagug     3600

aaaguuucua aggggaagau ccugacagcu acccggaaaa gccuggauac auccuuuaau     3660

gccaagacca uccuuucuau uacggacaca ggaauccaaa agauucugcu caacuaccug     3720

aaggccaaag gcaauccuga acucgcuuuc agcccggagg ggaucgaaga gaugaaccag     3780

aacauuuccc uguauaauga uggaaagccc caccagccua uccuuaaggu gcgcgucuuu     3840

gagcagggcu cuaaauuccc ccugggggaa accggcaaca agaccacaaa auacguggag     3900

gcggccaagg gaaccaaucu cuucuuuggc guguaugaaa acaaucaagg gaaaagaagc     3960

uacaacacaa ucccucugaa ucuggucauu gagcggcaga agcagggacu uaacuccguu     4020

cccgaaacga augagaaagg ccaucgccuu cucuucagcc ugucuccuaa cgaccucgug     4080

uaugugccca ucgaggggga agucuuugau aagaccaauc cggacgugaa cagaauuuac     4140

aagaucguuu ccuucagcgg aucucagaug uuuugcgugc ggcaagaugu cgcuacaucc     4200

auuguguaca aacuggaguu cagcucucuu aauaagaugg aaagaaccau cgacggcauc     4260

augauuaaag agaucuguau uaagcugaaa guugaucgcc uggggaacau cuccaaggcc     4320

uga                                                                   4323


<210>  4532
<211>  4323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4532
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc ccuuguggag       60

caggauuucg aaaauaaaca aggccugauc cucgggaugg gauccagaau uauccccaug      120

gacgcuggca uuaucgggaa guuugccgag ggaucuagca ucucccagac ggcggaacgg      180

accggcuacc gcucuauuag acggcugcgc gagagacacc uucugcggcg cgaaagacuc      240

caucgggucc ugaacauccu gaaauuccuc ccugagcacu augcugccga gauugauuuu      300

gaaaagcgcu ucggccaguu ucuggccgag acagaaccga aacuugcuug gaagaagaau      360

gccgagggga aauucgaguu ucuguuccag cggagcuuug acgaaauggu ugcugauuuc      420

aaggccaacg gacaagacau caaaauuccc uacgauugga ccaucuauua ccucagaaag      480

aaagcgcuga cacagaagau uuccccucag gagcuugccu ggaucauccu gaauuuuaac      540

cagaagcgcg gcuauuacca acucagaggg gaagaggaag aggagaaucc caacaaaaag      600

guggaguucu auucucugaa aauuguggac gucguggcug augaacagcc uaauaagaaa      660

ggagacaccu gguacagccu gcaucucgaa aacggcuggg uuuaccggcg cuccagcaag      720

aucccccugu augauuggaa agacaaggug agagauuuua uugucacaac cgacaucaau      780

gaggacgggu cugaaaaacu ugauaaagac ggagagguga agcgguccuu cagagccccu      840

aaagaggaug acuggacgcu gauuaagaaa aagaccgaac aggagaucga acaguucaac      900

caaacaguug gcagcuacau cuaugaggcu cuccuggaua auccgaccca gaagauucgc      960

ggcaaacuug ugcggacaau cgaaagaaag uuuuacaaau cugagcugaa gaaaauucuc     1020

gagaagcaga ucgaacugca acccgagcug uucaccgaag accucuauaa cgccugcgcg     1080

cgcgagcugu accggagaaa ugucgcccac cagcagcagc uuuccagccg cgauuuugug     1140

uaucuguucc ucaacgacau uaucuuuuac caacggccuc ugagaucuca gaagucccuu     1200

aucagcaacu guucccugga guuccgcacg auuaaagaua aggacgggaa uggacagauc     1260

auuuaccuca aagcuauccc caagucuaac ccuuauuacc aggaauuucg gguguggcaa     1320

uggcuguaua aucugaaaau uuucacaaag gaggaugaca aggacgucac caaccaguuu     1380

aucaauaaag uugaagaucu cgaggcccug uucgaauuuc uuaugaacca gaaggaggug     1440

aaucauacag acauucugac cuaccucauc gagcccaucg ugaaaagcaa guauccgaac     1500

gcuaaaggca aggccuucaa agaugaacug aagaaggagc ugaaaaagua cagauggaau     1560

uaugucuacg acgccgauaa agacgaaucc aagagcuacc cuaugaacga gacccgcuau     1620

gaacuucgga aaagacucga uaagguggag aauguucccg cugacuuuau gacacggaag     1680

auugagcagc accuguggca caucauuuac ucugugacgg auaaaaucga guucgaaaag     1740

gcgcugaaau ccuucgccaa caaguaugac cuccaucaag agagcuuugu cgaaaauuuc     1800

aaacgcuuua agccuuucga uucugacuac gggaccuaua gcgaaaaggc uauuaaaaag     1860

cugcuucccc ugaugagagu gggaaaauac ugguccuggg auaucaucga cgacaagaca     1920

aaaacccgga uuggcaagau cauuacaggg gaguaugaug agaccaucaa gaacagaguu     1980

cgcgaaaaag ccauugaccu cacggaggaa aaucacuuuc agggacugca gcuguggcuu     2040

gcccaguaca ucguguacga ucggcauucu gaggcugaca ucgccggcaa guggaacagc     2100

gucgcggauc ucgagcaaua ucuguccgag uucaaacagc acagccugag aaaccccauu     2160

guggaacagg ucaucacaga aacccuccgc gugguuagag acauuuggaa gcaauacggc     2220

aaaggggcua aggauuauuu ugacgagauc cacauugaac ugggacggga gaugaaaaau     2280

accgccgagg aacgcaagag acuuucuucc aucaucagcg agaacgaaac aaccaaucug     2340

cggauucgcg ccauccucga ggaacugaug uacgaugaga acauggagaa ugugagaccu     2400

uauucuccga ugcagcagga agcucugaag auuuacgagg acggcguccu uuccagcucc     2460

guggaaaucc ccgaggauau ugaaaagauc ucuaagaaaa gcgagccuac aaaguccgag     2520

auucagcggu auaaacucug gcuggaacaa aaguaccgcu cucccuacac ggggagaccu     2580

aucccccuga gcaaacucuu caccccggcc uaucagaucg agcauauuau cccucaggcu     2640

cgguuuuucg acgauggauu uuccaacaaa guuauuugcg aaagcgaggu gaaugcccug     2700

aaggacaaaa ugcuuggccu ggaguucauc cagaacuacu augggcaaaa ggucacaacc     2760

ucuuuuggcg aagugacaau ucucaccgag gacgcguaca aagccuucgu gaaggaacac     2820

uaugauaaga aucgcuccaa aagaaacaag cugcugcuug aggaaauccc cgagaaaaug     2880

aucgaccggc agaugaauga uacgagauac auuagcaagu auaucucuaa aguucucucc     2940

aaccuggucc gcggagagaa ggacgaugaa ggcgugaaua gcaagaacau uaucccuggg     3000

aauggaaaaa uuaccggcau ccugaagcag gacugggggc ucaacgaugu guggaacgac     3060

cugauccuuc cccgguucga gagaaugaau cagcugacaa acucuaccga uuuuacagcu     3120

uggaaugaaa aacaucaaaa guuccucccu agcguccccc uggagcuguc caaaggauuu     3180

cagaagaagc gcauugacca ccggcaucac gcccuugaug cucugaucau ugccugugcg     3240

accagagacc acgugaaccu ccugaauaac aaacaugcca agagccgcaa ugaacgguac     3300

gaccuccagc acaaacugag acauacgaca ccguggcaag auaaggaggg caaaguucgc     3360

accaaguuca cagaguuuaa aaagccuugg gaaaccuuca cccaggacac aaagaaagag     3420

cuugaaaaga ucguggucaa cuuuaaacag aaucugcggg ugauuaacaa ggcuacgaau     3480

uacuaugaga aaaucguuga ugggaagaag gugauguuca aacaggaagg cgucaacugg     3540

gccauuagaa agcccaugca caaagagacc gugucuggaa agaucgaccu cccccggguu     3600

aaagugucca agggcaagau ccugacagcu acccgcaaaa gccuggauac auccuuuaau     3660

gccaagacca uucuuucuau cacggacaca gggauucaaa agauccugcu caacuaccug     3720

aaggccaaag gaaauccuga gcucgcuuuc agcccggaag gcauugagga aaugaaccag     3780

aacaucuccc uguauaauga ugggaagccc caccagccua uccuuaaggu cagaguguuu     3840

gagcagggau cuaaauuccc ccugggcgag accgggaaca agaccacaaa auacguggaa     3900

gcggccaagg gcaccaaucu cuucuuugga gucuaugagg acaaccaagg caaacggagc     3960

uacgauacaa uuccucugaa uaucguuauu gaacgccaga agcaggggcu gaacuccgug     4020

cccgagacga augaaaaagg acauagacuu cuguucucuc ucagcccuaa cgaccuggug     4080

uaugucccca ucgagggcga gguguuugau gaaaccaauc cggacguuaa ccggauuuac     4140

aagguggucu ccuucagcgg gucucagaug uuuugcguga gacaagaugu ugcuacaucc     4200

aucguguaca agcucgaguu cagcucucug aauaaaaugg aacgcaccau cgacggaauu     4260

augaucaagg agauuuguau caaacuuaag gucgaccggc ugggcaacau uuccaaagcc     4320

uga                                                                   4323


<210>  4533
<211>  4332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4533
augaucaagc ugcucuaccu ggagaaacuu aagcaguaua ccaugaaaaa gauucugggc       60

cucgaccugg ggacaagcuc caucggaugg gcccuuguga acgaagcuga aaauucugau      120

gaagucagcu ccauuaucaa acugggcguu agagugaacc cccucacggu ggacgagaug      180

caaaauuucg aaaaggggaa gucuauuacc acaaacgccg aucggacccu gaaacgcagc      240

augagacgga aucugcagcg cuacaagcuc agacgggacg cgcugaucga gcuucugaaa      300

aaggucggau uuaucacaga uaaaaccauu cucuccgagc agggcaaccg cacaaccuuc      360

gaaacguaua gacugcgggc uaaggccgcc gaggaagaga ucucucuuaa ggagcuggcu      420

cgcgugcucc ugaugauuaa uaaaaagaga ggguacaaaa gcucccggaa ggccaaagga      480

gaagaggaag gcgcucugau cgacggcaug gagauugcca agaaacucua ugaugaagac      540

cugaccccug ggcagcuuug ccugcaacuc cuggagagcg gaaagaaguu uuucccggau      600

uuuuaccgcu cugaccuuca ggaggaguuc aacaaaaucu gggauuuuca gaagcaguuc      660

aaucuguuuu ccuucuguga cguugcgaaa gaggaaguga agggcaaaaa caagagccaa      720

acaugggcca uucucgcuaa guauuuuguc gagaccaaaa aggugaaugu uuggaacgaa      780

cacgaggccc agacagagaa uagagaagag guguacaaac uggucgggcu gaagcggacc      840

gugaaaggag aagaacucaa gaaggaaaac uaccgcugga gagugcaggc ucugacggag      900

cagcuugauc uggagaaaau cgccgucguu cuccaagaaa ucaauggcca gauuucugcg      960

uccagcgggu aucugggagc caucucugac cgguccaagg ugcuuuacuu caacagacag     1020

acagugggcc aauaucagau ggcugagcug aacaaaaauc ccaacaugag ccuccgcaau     1080

auggucuuuu accggcagga cuaucuggau gaguucaacg ugauuuggga gaagcaggcc     1140

gaguuccaua aagaacugac cccugagcuc aagaaggaaa ucagagacau uaucaucuuu     1200

uaccaacgcc ggcugaaauc ccagaagggc cuuauuucuu ucugcgaguu ugaaaauaga     1260

gagguugugg ucgagaaaga agggaagaaa cagacaaaga ucauuggaug uaaagugauc     1320

ccccgcagcc acccucuguu ccaggaguuu aagauuuggc aaacccucaa caauaucgaa     1380

guuuucgugg gcgagaagaa agggaagcgg aaaaagucca acgucagcuc ugaucuguuu     1440

ggagacaccg aagaugcucu gaucacagag ggcagacgcu uccuuuauca ggaggagaaa     1500

aagauucucg ccaaggaacu guccaucaaa aaggggcuga cgaaagccga aauucuccgg     1560

cugcuuuuug agaaucccca ggaacuggac aucaacuuca agcagauuga uggaaaucaa     1620

accggcuuug cucucuucag cgcguacucu aacaugaucg agaaauacgg cuaugagccu     1680

cuggacuuua agaagccggc cgaagaucug auuagccagc uuaaaacagu guucaccgac     1740

cucgggugga auacagaucu gcuguucauc gaccucgaca aggagaacaa agaaugggag     1800

aagcagcccu acuuuagacu guggcaucuu cuguauuccu ucgagggaga uaauaccccu     1860

acgggcaacg ggaaacucau cgaaaagauu augcagcugu gcggaaucga gaaggaauac     1920

gcuauugagc uggccaacgu cucucuucaa gacgauuaug gcagccucuc cgccaaagcu     1980

aucaagaaaa uucugcugua ccucaaggaa gggaaucagu augacauggc cugugaguac     2040

gcgggcuacc gccacagcaa aucuucccug acaaaggagg aaaucgagaa caaagugcuu     2100

aaggauaagc uggaaauccu ccccaaaaau agccugcgga acccuguugu ggagaagauu     2160

cugaaucaga uggucaacgu gaucaauacc guuauggaag uguauggaag acccgacgag     2220

auucgggucg agcuugcucg cgaacucaaa aagucugcca aagagagaga ggaacugacc     2280

aaggccgugg cuaagaacac acgggaacau gaggagauca gaaaagugcu gcaauccgaa     2340

uuuggcauga uuaauguuag ccgcaacgau aucauccggu acaagcucua ugaggaacug     2400

aaagacaaug gguacaagac ccuuuauucc aaccaguaca uuccgagaga gaaacuguuc     2460

ucuaaggaga ucgauauuga acacaucauu ccucagagcc gccucuuuga cgauucccug     2520

ucuaauaaga cacuggagua uaaaagcauc aacaucgaaa agggaaauaa aacggccuac     2580

gacuucguca aggagaaaua cggcgaagag gggcuucagc aauaucugaa cagaugcgag     2640

aaccucugua aggauaagaa agcuaagcug cggaaacuca agauggaacu gaaugagauu     2700

cccgacggau uuaucgaccg cgaucuuaga aacacccagu acauugccaa aaaggcgcug     2760

uccaugcuca cagaaaucug ccggcgcgug guggccacca caggcagcau uaccgacaag     2820

cugagagagg auuggcagcu ggucgacgug augaaagaac uuaaucugcc uaaguaugag     2880

gcucucgggc ugguugaggu guacgaagau aaagacggcc ggaagaucaa acgcauuaag     2940

gauuggacga aaagaaacga ccaccggcau cacgccaugg augcucucac cgucgccuuc     3000

acaaaggacg uguuuaucca guauuucaau aacaagaaug cggccuggga gucugauucc     3060

aaagaacaua ccaacaucgu uggaauuaag acacgcuacu uugagagcgg caaagcugug     3120

ccucccaucc cccuggggca auucagaguc gaagccaagu gucaccuuga gaaucugcuc     3180

gugucuauua aagcuaagaa caagguugug accacgaaug ucaacugcau caaaaagaaa     3240

ggaggcacau auaagaauac ccagcagaca ccgcgggggc agcugcaccu ggagaccauu     3300

uacggauccc auaaccaaua cgugaccaaa aucgaaaagg ugaaugccag cuuugacgcc     3360

gucaagaucg cuacaguuuc uaaacaggau uauagaaacg cgcuucugaa gcgccucgag     3420

guguucggca acgaccccaa aaaggccuuc acggggaaaa auagccugga aaagaacccu     3480

auuuaccucg accgggcuca gaccauccaa gugcccgaua agguccagac aguggagcug     3540

gagaccgccu auacaauuag aaaaccuguu gaccccaccc uuaaugugga uaaggucgug     3600

gacaaaaaga uccgccugau ucuggaacag cggcucaaag aguacggcgg agaugccaag     3660

aaggcuuuug uuaaccugga cgaaaauccg aucuggcuua acgaggaaaa aggcaucucc     3720

auuaagagag ugacgauccg cgggauuagc aaagcccagg cgcugcacga uaagaaagac     3780

aaggauggaa auuucauccu cgacgagaca ggcaaacgga uuccugucga uuuugugaac     3840

accgggaaua accaucacgu ugcuaucuau agaaagcccg ugcuggacaa gaaaggacaa     3900

gucauuuucg acgaugaggg caauccuaug uacgaacucg acgaggaugu ggugcccuuu     3960

uucgaagccg ucacccgcgc caaccugggg aucccuauca uugacaagga uuauaaaaag     4020

ucugagggcu ggcaguuucu uuucuccaug aaacagaaug aauacuuugu uuuccccaac     4080

gagaagacag gauuuaaucc gaaggaggug gaccugcugg auccugacaa cuacgcuaug     4140

aucagcccca aucucuuccg ggugcagaaa auuggccuga acgauuauac cuucagacau     4200

caccuugaaa caacgaucca aaacgaccug aaguuuauua ccuggauccg gugugggaaa     4260

uccggaauca aggauauugu caaagugcgc aucaaucaca uuggccagau cguuucugug     4320

ggggaguacu ga                                                         4332


<210>  4534
<211>  4332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4534
augaagaaaa uccugggccu cgaccugggg accgccagca uuggauccgc uuacgugcuu       60

gaggccgaaa agucugcgga ucagacaaaa aucgucuauc ugggcacgag aauuauccuc      120

agcuccgcug acgccgagaa cuucucuaag gggcugagcu gcucccccac cacagaucgg      180

accuuuaaac gcucuaugag acggcgccuu caaagauaca agcagcggcg cagcucccug      240

auucagcagu ucaagaaaau cggaaucauu acaaauaccg aacaaaucau uagagagccu      300

aucagcgaaa cacacgagcu ccugcggauu cgcucuaagg ccguuuccga gcaugugagc      360

cuggaagagu uugcuagaau ccuccugacc auuaacaaac ggcgcggcua uaagucuaau      420

agaaaaacgu ccaagagcac cgaagaggac gggaaacuug uggaugccau ggcugucaca      480

aaggagcuga ucaagcucaa aaagaccccg ggacaguaca uguucgaacg guuuaaacgc      540

ggcaaguaug ccgagaucga cuucuacccc uuugaucuga uuaacgaacu uaaacagauc      600

ugggaggugc agggccaaaa guauccugac guucugacag aagggcuccu gucuucccug      660

cucaagcaga aaaccggaaa ggcgaaagag cugauuuaca gcaaguccga ucuuaauccc      720

gccgaggugc cuaucgacua ccagaaaggc aagaagcuga gaaaaacgau ucucaacaag      780

uauuucgcuu acgaaucucg gagccuggcc gucacaaaua ucauccccgu ggagcaggcu      840

auuaccgcca ucguugcgau uuguaaacaa aucaacaauu ccagcgggua ucuuggagcc      900

auuggcgaua gaucucgcgu gcuguacgcu aacaaucaga caaucgggca guaucucuac      960

gaacggauca aggccaaccc ucacgaccgg auucgcggag aggucuuuua ucggcaagau     1020

uaccuggacg aguucgacag aaucugggaa ugccagcaga auuacuaucc ggcuauucug     1080

accaacgauc gcaaacagua cauccggaau agaauuaucu uuuaucaacg cccccucaag     1140

uccaagaaaa gcgagcuggc cuucugugaa uuugagucuc gggaagugga gcauaacggc     1200

aagaaauaca ccgaaggccu ucacguggcc ccuaagagcu cccccgucuu ccagcauuuc     1260

agacuguggc agcgccucgg gcagauugag guuucuccuc ugcggaaaug ggacaagcaa     1320

agcaacgagc uuuuugaaca gcagaauaac gcugauagag agggaauccu gaauacagaa     1380

gcgaagcaga aacucuucuc cuaucugaac cugcucgacc accugacgga uaaugagauc     1440

cuuaacauuc ugggcuacag ccccgacaag uggaaaauca auuuucggga gauuaucggg     1500

aaccgcacca auaaccucau gaugaaggcc uucuuuucua ugaaagaaau gacagaugga     1560

gagcaaauug acugggaaaa gaaucugacc aaaaucacaa acggccaguc ccugaucagc     1620

ccggagcaga uuguggaucu uaucagacag uucuuugaac gggaggggau uaccacgucu     1680

cuccuguauu ucgacccugc uacagauaau uuuagagagc aaucccacua cagccugugg     1740

caucuccugu acuccuauga agacgacaac ucucccaccg gaaccacaag ccuuugcaag     1800

acccuggcca agcgcuacgg caucccucac gagcucuguc cccugcuggc cucccuuaca     1860

uucgaacggc aguauggcuc ucucagcacg aaagcuauga gaaagcugcu gcccuuuaug     1920

caggagaaac gcuacgauga agccugcgag cucgcggggu auagacauuc cgcuuggauu     1980

accaaggagg aaaaugccaa acggacacug aagaacaaac uuccucugcu ccgcaaggga     2040

agccugagaa acccgguggu cgagaagauc cugaaucaaa ugacccacgu gaucaacaca     2100

cacauugccg aauacggcaa ucccgacgag auccggauug aaauggcucg cgauaugcag     2160

gccaacucua aacagagaaa ggcuaugacc gccgcgauca cggaggccac caaacggaau     2220

acacaguuca uugaggaaau ccaaaaggag uuuggguuca acccuuccaa gaaugaccuu     2280

aucaaauaua agcucuggaa agaacuggcu ccccugggaa acaagacccu cuacagcaau     2340

auuuacaucc cuaaagagga gcuguucaca gccaaguuug aaauugagca uaucauuccc     2400

cgcgcucuuu ucuaugauga cucuuuuucc aacaagaccc uggaacucaa uagcguuaac     2460

aaacugaagg gcaauaaaac ggcccuggau uaccuugaca cacucgggca ggagaaggcg     2520

aacgauuaua uccugagagu ggaggaacug aaaaagauug gcgccaucac ccaggcuaaa     2580

uacaagaagc uccugauccg ggagaaagac cuuggagaug acuucacaaa ucgcgaucug     2640

accaacacca gauauauuau ccggcacgcc gcugacagag accucacaaa cacgcgcuac     2700

auuauccggc augccgccga uaugcugcug caggucaccc cgucuauugu gcacacaaau     2760

ggcagcauca ccgcuauccu uagacgcgac ugggggcugc ucgauauucu gaaggaacuc     2820

aaccugccua cauaugaguc caucgaccgg aaaauuagcg aucccgaaga gaaacuuacc     2880

caaucuguug acagaaagga uggacaggcg aucaagauga uuaaggacug guccaaacgc     2940

gaugaccauc ggcaccacgc cauggaugcu cugaucacgg cccucacaac cagaaagcau     3000

gugcaguacc ugaauggcaa cagcggggcc gaagagaccg acaaagcuca gaagcuggag     3060

caacuuaaag cccugcgcaa gcagcucauc ggauccgaug gccacuuuau uccucccaug     3120

caugacaucc ggguccaggc gaaggaagcu cugucuagag ugauuaucag cacaaaaguc     3180

aaggggaaag ugaccacacc uaauacgaac auuaucuguu cccgggacaa gaccacaggc     3240

cgcaaaauuu acguuagaca aaccacacuc acccccagag gagcccugca ccuugauacg     3300

aaguauggca ucaaucgcua cuaucaggag agagaagugu cuaucgggag caagcugacc     3360

caggaggaaa uucggcucgu cuccucuccg cacauccaga gcauucugcu ggagagacuu     3420

cgcgccuucg acaacaaucc uaaauccgcu uuuacaggac ggaacagcgu ggauaagaaa     3480

ccccuggagg acaagaauaa aaagcucguu uacaccgaua agaaagugaa gcaaacaauc     3540

cuggccaaca gauucgucaa acgcgaccgg guuaauucua agcucgaucu ggacaacgug     3600

auugauaccg gcaucaaaag aauccuugaa cgccggcuga agcaguaugg gggaaaauac     3660

gagacagcgu uuuccgaccu ggaaaauaac cccauuuggc ucaacgagga gaagggcauc     3720

gcuauuaaga gcgugagaau ccgcgggaaa aauaacacca uuacgaucag acagacaaau     3780

accugccgga ucaaguaugu cgccaacgau aauaaccauc accuggccgu guacgcccuu     3840

ccugacggcu cuauuaaaga cgauaucgug uccuucuuug acgcugucga acgcagcaga     3900

aagcggcugc cgauuaucga uaaacauaag aaucagucuc ucggauggaa guaccuguuc     3960

uccauuaaac aaaacgagua uuuucuccug ccccaacagg uggaaaagau cgacauugau     4020

accggcgaga ucagcaugca gacaaccuuc uccccuaaag accuuagcca cgaauacauc     4080

augaaucccg auaacuaugc ugagcuggcc ucucauauuu uccgcguuca gagcaucucc     4140

aagacgccuc aggggaauag agacgugcgg auuuaccugu uuaaacagca guauaucucu     4200

ggaagccaaa aggauucccu ccaccugaag ggcauuacac acagacagau cucuagcgcg     4260

aaagaccuug ccgagcuggu caagauccgc gugaaccauc ucgggcggau uguuuccgug     4320

ggagaaaaau ga                                                         4332


<210>  4535
<211>  4341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4535
auggugaaga acauccuggg ccucgaccug gggaccaaua gcgucggaug ggcccuuauu       60

aaacaggauu ucgagaacaa gcaaggcgac auccugggga ugggauccag aauuaucccc      120

augucucagg auguucucgg cgacuuuggg aaaggaaaua gcauuuccca gacagcugaa      180

cggacgaagu accgcucugu gagacggcug cgcgagagau uccuucugcg gcgcgaaaga      240

cuccaccggg ugcugaacgu ccuggauuuu cucccugagc auuaugccag ccagaucgac      300

uucaauaaac gcuuuggcaa guucaaggaa gagaccgagc cgaaacuggc guacuccaac      360

gauggcuuua ucuucaagaa aucuuuugaa gagaugauua gcgacuucaa gacacaccaa      420

cccgaacuuc uggagaauaa caaaaaggug ccuuaugauu ggaccaucua cuaucucaga      480

aauaaggcuc ugucccauaa aauugagaag gaagaacuug ccuggauccu gcucaacuuu      540

aaucagaaac ggggguacua ucagcugcgc ggagaagagg aagaggagaa ccccaauaag      600

cugauugagu ucuacagccu caaaaucguu gacgugcugg ccgaugagcc ucagaagggc      660

aaaucugaca uuugguacuc ccuuauccug gaaaacgggu ggaucuauag acggagcucu      720

aagauucccc uccuggauug gaagggaaaa acacgcgacu uuaucgucac cacagaccuu      780

aaugaugacg gcuccgugaa gaccgauaaa gaggggaacg aaaagcggag cuucagagcu      840

ccuggagaga acgacuggac gcugguuaaa aagaagaccg agcaagaaau ugauaauucu      900

gacaaaacag ugggcgccua caucuaugag gcucuccugc agaacccgaa gcagaaaauu      960

aagggcaaac uggucagaac caucgaacgc aaguucuaca aggccgagcu caaacagauc     1020

cuggaaaagc aaauggaguu ucaccccgag cuucggcagg augaccugua uuccgauugc     1080

gugagagaac ucuacaaaaa uaacgaggcg caucagcuga cacuuagcaa gaaagacuuc     1140

gugcaccugu uuauggaaga uauuaucuuc uaucaacgcc cucuccgguc ccagaagucu     1200

agcauuucca auuguacccu ggaguuuaga aaguacaaag acgagaacgg gacggaacac     1260

auccaguacc ugaaggcccu ccccaaaagc aauccuuauu accaggaguu ccgcauuugg     1320

caauggaugu auaaccugaa uaucuacaag aaagaagacg auacaaacgu caccaaggag     1380

uuucuuaauu cuaaagcuga cuucgaaucc cuguuugagu uccucaacaa uaagaaggag     1440

guugaucaga aagcccugcu gaagcauuuu aaaaucaacg acaagacaca ccgguggaaa     1500

uauguggaag auaagaagua ccccugcaau gagaccaaaa ccaugauuag cgaaagacuu     1560

aagaaagugg agaacaucuc ugacgauuuc cucacacgcg aaauggagca gaagauuugg     1620

cauaucauuu auagcgucaa cgacaaagug aaguacgaga aggcucugaa auccuuugcc     1680

gaaaagaaua accuggauga gucuagcuuc uucgaagcgu uuaaaaaguu cccgccuuuu     1740

aaauccgagu acggagccuu cagcgaaaag gcuaucaaga aacuccugcc ccuuaugcgg     1800

cugggcaagu auugggacga ugacgagauu cucaaauacu cugauaucua uuuuaagaau     1860

aucgaguacc ugcugggggu ucuuaaacag aaggacaaaa acauuuccga cgauaauaga     1920

caaaacugga auaagagcau caacaauaag cuccgcgaag aacuggaaau uuuccagaac     1980

gccgagaucg agucuuuuca gggacugcgg cuccacauug cucaguauau ccuguacggc     2040

agacacuccg aagccgccau gcuugggaaa uggaauagcg cugacgaucu ggaggaguuc     2100

cucaaggagu uuaaacaaca uucccugcgg aacccuaucg uggaacaggu cauuacggag     2160

acccugcgcg ugguuaagga caucugggug aaauauggaa auggcgcgaa ggauuucuuu     2220

aacgagauuc acaucgaacu uggcagagag augaagcucc ccgccgaaga ccggaaaaag     2280

auuacaaauc agaucucuga gaacgaaaac accaaucuga gaaucaaagc ucugcucgcc     2340

gagaugaugg ucgauagcuc cguggagaac guccgccccu ucucuccuau gcagcaagaa     2400

auucugaaga ucuacgagga cggggugcuu aauagcgaua uugaaaucga ggacgauauu     2460

cugaaaaucu ccaagacagc ccagccgagc cccucugacc ucaagcggua caaacugugg     2520

cuggaacaga aguauaaauc cccuuacacc ggacaaauca uuccccuuaa caagcucuuu     2580

acgccugagu augagaucga acauauuauc ccccagagca gauacuucga ugacucuuuc     2640

uccaauaaag uuauuuguga ggcugccgug aacaagcuga aggacaauuu uaucggccug     2700

ggguucauua aacaguuugg aggcacaauc gucgaaugcg ggaacggcaa gagcgugacc     2760

guucucaaag aggaucagua ugaggacuuc gugaagaaac acuacgcgaa uaaccgcucu     2820

aagagaaaua agcugcuucu ggaagauauc ccggagaaaa ugauugaacg gcaaaugaac     2880

gacacccgcu auaucagcaa guacauuucc ggaguccuca gcaauaucgu gagaacagag     2940

gauggcaccg acgauggggu gaacucuaaa aauauuguuc cuggaaacgg caagaucaca     3000

aacacgcuga aacaggacug ggggcugaau gauaucugga acgaccuuau ucugccccgg     3060

uuugaacgca ugaaucagcu caccaacucc acagauuuca ccgcuuggaa ugagaaccau     3120

cagaaguuuc ugccuacagu ccccguggag uucagcaaag gauuuuccaa gaagagaauc     3180

gaccaccggc accaugcccu cgacgcccug gugauugcuu gugccaccaa agaucacguc     3240

aaucuucuga acaaucaauc ugcuaagagc gacacgaaac gccaugaucu caagagaaaa     3300

cugaugaagu ucgaaaaggu ggccuacaac cacccucaga ccggcgagcg gaucgaacgc     3360

gagguuccca aacaguuucu gaagccgugg gaaacauuca ccguggaggc gaaaaauggg     3420

cuugagacaa uuaucgucuc cuucaagcag aaccugagag ugaucaauaa agccaccaac     3480

cacuaugaaa aguacguuga gaaggacggc gcuaaaguga agggacgggu cgaacaagcc     3540

ggcacgaauu gggcuauuag aaaaccuaug cauaaggaga cagugucugg gaaaaucgau     3600

cucccccgca uuaagguucc caagggaaaa auccugaccg ccacacggaa gaaccucgac     3660

accagcuuug aucugaaaac cauuggcucc aucacagaca cggggauuca gaagauccuu     3720

aaaaauuauc ugcaguacaa ggauaacaac ccugaacucg cguucucucc ggagggaauc     3780

gaggacauga auaaaaacau ugaaaaguuu aaugauggca aggugcacca acccaucauu     3840

aaagucagaa ucuucgagcu ggggagcaag uuuccucugg gccaguccgg aaacaaaaag     3900

gacaaauaug uggaagccgc uaagggcacc aaucuuuucu uugccgugua cgaggauaag     3960

aacgggaaac gcagcuauga gacaauuccc cugaaugaag ucaucgagcg gcagaagcag     4020

ggacucaccg uuguggaccu gaaaggcgug aacgacuucu accucucucc uaaugaucug     4080

gucuacgugc ccuccgaaga cgagcuugaa aacguuagca ugaucgauua uaauaagcug     4140

ucuaacgaga aaguguacaa ggucgugucc uuuacaggga aucaaauuuu cuuuguuaga     4200

caggacgugg cuaccagcau cgucaacaag gccgaguucu cuucccugaa uaaaauggaa     4260

cgcgccauug gaggcacgau gaucaaggag guguuuguua aaauuaagau cgaucggcuc     4320

gggaacauca gcaaagugug a                                               4341


<210>  4536
<211>  4347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4536
augaagcaca uccugggccu cgaccugggg accaacagca uuggaugggc cguggucaaa       60

gaggcucaga augauaacga acaauccgag aucguugacg ugggcgugag agucaauccc      120

cuuacaacgg augaaaagac caacuucgag aaagggaagc cuauuucuac aaaugccgac      180

cggacccuga aacgcagcgc gagacggaac cuccagcgcu acaagcugag acggaaggaa      240

cuuaucgagc ugcucaaaga gcaucagcug auuuccgaaa auacaauccu gaccgagaac      300

ggaaagggca caaccuuuca gacgcucgaa cugcgcgcua aaucugccaa ggagaaaauc      360

agccuugggg agcuggccag aguguuccuc accauuaaua agaagcgggg auauaaaucc      420

ucucgcaagg cuaaaaacgg cgaugaaggc caacugaucg acgggaugga gauugccaag      480

agacuuuacg aagagaaucu gacaaccgga cagcucagcc ugcagcugcu caaagaaggc      540

aagaaauccc ugccggauua uuaccggagc gaccuucagu uugagcugga uaagguuugg      600

aacuuccaaa agcagucuca cuccgacauc cucacagaug aguuuuaugc ucagcugaaa      660

gggaagggac agcgcgccac cagcgcgcuu uuccuggcca aauaccaaau uuauacggcu      720

gaaaauaagg gcucuagaga ggacaaaaag cuccaggccu accaguggcg gguggaugcu      780

cuguccaagg aacugacacg cgaggaacuc gccuuuguca ucaccgagau uaacaauaac      840

cugaacaaua gcucugggua ccuuggagcg aucuccgaca gaagcaaaga gcuguauuuc      900

aacaaugaaa cagugggcga guaccucugg aagcaaaucg ccgcuaaccc ccauaauucc      960

cugaaaaacc agguuuucua ucggcaggac uaccuugaug aauuugagca gauuuggaag     1020

acccaaucug ccuuuuaucc ugaaauccug accgacgagc ucaaaaagga gcugagagau     1080

guggucauuu ucuaccagcg caagcugaaa agccagaagu cccucgugag cuuuugcgag     1140

uucgagaaua aagaaaucga gauuaagaac gaggacggca caacgaccaa aaaggugauc     1200

gggcugaagg ucgcucccaa aucuuccccu cuuuuccagg aauuuaagau cuggcaaaau     1260

cugaacaaug uucucauucg gaaaaaggga agcaaaacaa agaaaguggc caagaaccag     1320

caggccgugc uguucgagga agagaagacc aucucugaac uggaucuuga ggcuaaacag     1380

gcgcucuuua augagcugaa ccugaagggc aaucucaaag ccgacuauug ucuggaaauu     1440

cuuggguaca gccccaagga gugggaaaug aacuacucca uccuggaggg aaacagaaca     1500

aauaaagcuc ucuaugaagc cuaccugaag auuaccgauc uggagggcua ugacgccaag     1560

aaccaccuua aagucaagcu caauaaagau gagguggaac uggacgaucu gaagguuccu     1620

gcuucugaga ucaaagacau ggugcaagcc aucuucaagg aagcggggau uaacacggac     1680

auccuccauu uuaaugcuga gcuggaugga aaggacuucg aaaaccaggc cagcuaccag     1740

cuuuggcauc ugcucuauuc cuacgagggc gaugacagca aaucuggcaa ugagcugcug     1800

uauaaacuuc ucgaaacaaa auuuggguuc aagaaagagu acgcccagau ucuggcuaac     1860

gucucccuga gcgaugacua cggaaaucuc ucuaccaagg ccaugcgcga aaucauuacc     1920

caccugaagc aaggcuuucc guucacaggg cggaaagaua gacccgacga gccuuccgcu     1980

ugcgaguuug ccggauauaa gcacagcaaa cacucccuua ccaaggaaga gaacgaaaaa     2040

cgcgugcuga aggauucucu cgagguucug aaaaagaaua gccugcggca gcccgugguc     2100

gaaaagaucc uuaaccagau gauuaaucuc gugaacgcgc ugcugaaaaa guacagaaca     2160

gagaauccua acuucaaauu ugacgagauc cgcaucgaac ucgcccggga gcugaagaaa     2220

aaggcugaag agagagccaa caugacggcu aauauuaaca aggccaaaac cgaacaugag     2280

aagaucauua aaauccuuaa gaaugaguuc ggccugccca acccgacacg caaugauauu     2340

auccgguaua gacucuacga ggaacugaaa gacaacgggu auaaggaucu guacaagggc     2400

gacuauaucc cucgggaaaa acuuuucucc aaggagauug acaucgaaca cauuaucccc     2460

aaagcgcgcg ucuuugauga cucuuucagc aauaagacca uuacauacag aaaaaccaac     2520

cuggagaagg gagagcggac ggccuacgau uauaucgaau cccucggcga gaagcaacug     2580

gaagaguuuc ucagcagaau cgagaaucug uacgaaauug ggaaaccuuc ucgccuucac     2640

aaccggagaa auccccugaa gaaaguggag gaaaagggaa ucuccgacag caaauauaag     2700

aagcuccuga aaaaggaguc ugaaauuggc gauggguucg uugagcgcga ccugagagag     2760

acccaguaca ucgcuaaaaa ggccaaagaa auucuucugc agaucacacg guccgugcuc     2820

agcaccucug gaggcaucac agaucgccug agagaggacu gggaucucau uaacgucaug     2880

aaggaacuga aucuucccaa auaucgggcu cuggggcuca ccaagaugga ggaccgcaag     2940

uacggccagc aaguggaagu uaucacggau uggacaaaaa gaaacgaccu gcggcaucac     3000

gccauggaug cccugaccgu ggcuuuuaca aagcauaauc acauucagua ucuuaacuac     3060

cugaacgcgc gcaaaaauga gaagcauaaa gagcacaaca auaucauggc cauucagcag     3120

cucgaaacca ucaaggucac cgacgagaac ggaaaugaaa agagaauuuu caaagcuccu     3180

augccgaacu uucggcaagu ggccaaggcc uuccuggaga augugcucau cagccacaaa     3240

gcuaagaaca aaguugucac aaagaauaag aacaaaaucu ccggcaauaa caaggugcag     3300

gaagugcuga cgccccgcgg gcagcuucau aaagagacca uuuacggaaa guaucaguac     3360

uauguccaaa aagaggaaaa gaucggcggg aaguuugacc uggagacaau ugaaaaagug     3420

gccaauccuc uguaccagag ccuccugaag aaaagacuug aggagaacgg aggcgauccc     3480

aagaaagcgu ucaccgggaa aaauucucug gcuaagaacc cuaucuaucu cgacgaagcc     3540

cagacagagc aacugcccga aaagguuaag cucguguggc uggaaaauuc cuuuagcauu     3600

cggaaagaua ucaccccgga aaacuucaag gacgagaaac uuaucgauaa gauucuggac     3660

gagggcguca aaagaauccu gaaggaacgc cuccaguccu uuggaaacga ugccaagaag     3720

gcuuucucug accuggagaa gaauccuauu uggcuuaaca aagaaaaggg caucgccauu     3780

aaacggguga cgaucagcgg gguugcgaau gcugagcccc ugcacacaaa gaaggaucau     3840

cucggaaaag aaauccugga caaggagggc aaaccuauuc ccguggauuu caucuccacc     3900

gggucuaacc accacgucgc cguguacaga gacgagaagg gaaaucucca ggaaaaaguu     3960

gugagcgucu uugaggccgu ggugcgcgcu aaccagggcc ugccuauuau cgacaagacc     4020

uauaaucaau accuugggug ggaguuccug uuuuccauga agcagaacga aauguucguc     4080

uuucccaaug aaucuacagg cuucaacccg aaugauauug accugcucga uccuaaaaac     4140

aagaaaauca uuagccccaa ucuguuucgg guucagaaac uugccaccgg agacuacugg     4200

uucagacauc accuggagac aacgguggag accaaguccg cuacaaagga uaugaccuau     4260

aaacgccuca gccugaacca uaucaagaau aucauuaagg ugcggaucaa ccaccucggc     4320

gacauugucu cuguggggga auacuga                                         4347


<210>  4537
<211>  4353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4537
augaagcaca uccugggccu cgaccugggg agcaacucca uuggaugggc cuucgugcag       60

caagauuuug agaauaaaca gggcaagauc auugcuaccg ggucuagaau cauucccaug      120

gaccagggaa uccuuggcga uuucgaacgg gggaacacag ucagccagac ggccgagcgc      180

accacauaca gaaauacccg gcgccugaga gaacggcauc uccugcgcag agagcggcuu      240

caccgcguuc ugcauauccu cggauuucug ccuccgcacu augacuccca aauugauuuc      300

acaaaaagau acggcaaguu uaucgacaac gcggaaccca aaauugcuua uaauaacggc      360

aauuucaucu uuaugaacuc uuucaaugag augauugagg auuuuaagaa guaccagccu      420

cagcuguucu auaaaaagag caacggggaa gaguccaaaa uccccuacga cuggaccauu      480

uauuaccucc ggaagaaagc ccugucucag aagaucacac aacaggaacu ugccuggcug      540

auccuccauu uuaaucagaa gcgcggauac uaucagcuga gaggcgagga ggaaaccgag      600

aacccuaaua aagaaguggc uuuccacagc cuugaggugg ucgaugugga agccgaggcu      660

cccaacaaga aaggggagau uugguacacg auccggcugg aaaauggaug gauuuaucgc      720

agaaccucca agaucccucu cgacgauugg aaaggcaaga cacgggacuu uauuguuacc      780

acagaucuga acgacgacgg gaccaucaaa cuggauaagg agggaaagga aaaacgcagc      840

uucagagccc cggacgagaa cgauuggaau cuccugaaga aaaagacgga acacgacauc      900

gauuucucug gcaaaacagu gggcgcguac auuuaugacc aucuucugca aaaccccaau      960

cagaagauca aggggaaacu cguccggacc auugagagaa aguacuauaa agaugagcug     1020

aaggccaucc uugcuaaaca gauugcccug caaccugaac ucuuuacaga gacccugcug     1080

gcugacugca uccgcgaacu cuaccggaag aacgagaccc agcagcagaa ucugcuuucc     1140

aaggauuucc ugcaccucuu uguggaagac aucauuuucu accaaagacc ccugcgcagc     1200

aaaaagucua caaucgccaa cuguacgcuu gagaaacggu ccuauauuga uaaggagacc     1260

aaugaaagaa aagacacacc uaucaagguu ugcgcuaaga gcaaccccua cuaucaggag     1320

uuucgcgugc ugcaguggcu ccagaaucug cggauuuacg aaaucgagac cgacagagag     1380

gucacagccg aguucauuga aaccgcucaa gauaacgaag cccuguuuaa uuuccucaug     1440

ucucagaaag agaucgacaa cgaaucccug cuuaaguauu uucuguucgc uaaaaauccg     1500

cagaucaagg auaaaguggc caagagcgag cucaaaaagu ggcugacgac auaccgcugg     1560

aacuaugugu acgacgccgc ugauaauucc ucuaagaaau acccuaugaa cgagaccgga     1620

uaugaacugc agcaauaccu ucagaagguc gagggccucc ccgcgaacuu ucugaccuuc     1680

gacauuacau aucggcugug gcaucuccug uacagcguua aagauaaggu ggaauuugag     1740

aaagcccuua agaaguucgc ucaggaauau cagcuggacg aggccuccuu cguggauagc     1800

uuuaaaaauu ucaagccuua ccccaacgag uaugggucuu uuuccgaaaa agccaucaag     1860

aaacuccugc cccugaugag auucggaaau cacugggacu uuaaccggau ugaugucagc     1920

acccaaaagc gcaucgacga ucuuauuaau ggcguggcua acgacgagau cagaacaauc     1980

auucgggaaa aggccgagaa auaccagcuc aauucugaaa cguguuucca ggggcugccu     2040

cuguggcucg cgcaauacgu uguguauaac agacacgcug aggccgccga caucgagaag     2100

uggaccagcg ucaaugaucu ggaagcguac cuugaggacu uuaaacagca uucccugcgc     2160

aacccgauug uggaacaggu uaucacagag acccuccggg uggucagaga uauuuggcag     2220

cgcuauggag gcggccaagc caauuucuuu gacgagaucc acaucgaacu ggggagagag     2280

cugaagaaaa cagcugagga acggaagcag cuuucuaacc gcaaucagga aaacgagaau     2340

accaaccuca gaauuaagaa acugcuggcc gagcucaagg aagaugcgag cauccagaac     2400

gugcggcccu acuccccugu ccaacaggag cugcuuaaaa uuuaugaaga gggaguguuc     2460

agcgccacgg acgaaguuga ggaggauauc cagaagauuc gcaaaaaggc ugaacccacc     2520

acaccugaga uccagagaua caaacugugg cucgaacaaa aguauaaguc ucccuacacc     2580

ggcgagguga ucucccugac aaaacuguuu accgaagagu acgagauuga acauaucauu     2640

ccgcagagcc gguauuacga cgauucucuu uccaauaagg ucaucugcga gagcgccgug     2700

aacaaacuca agacgaauca gcuggggcug gaguucauuc aaaaccacca uggagaaaaa     2760

guucgcauca cagcuaauaa ggaggugacc auuuuuacag aggaacagua uaaggaguuc     2820

gucaaaaagc acuacgccaa caauuccgcg aaacagcaga agcuccugcu uaacgaaauc     2880

ccugagaaaa ugguggccag acaacugaau gacacccggu auaucucuaa guacauuagc     2940

gaaauccucu ccaagauugu gcgcucugau gagaaagacg aaggcguuaa cagcaagaau     3000

aucauucccu guaccgggaa aaucacaacg gcucugaagc aggauugggg ccugaacgac     3060

gucuggaaug agcuuauccu gccuagauuc gaacggauga accagcucac ccagacacaa     3120

cuguuuaccu ccuauaauga cagauuccag aaaaaccucc ccacagugcc uauugaguac     3180

agccagggau uucagaagaa gcgcaucgau caccggcauc acgccaugga cgcucuggug     3240

auugccugcg ccaccagaga acauaucaac uacaugaaua accaacacgc ucuugauaaa     3300

ucuaaggaca aaaaggagaa acagcagucc cgcauugauc ugcggacgau ccucugugag     3360

aagaagaaua acaauuggca guucaaaaag cccuggagca cauuuaccca agaagcgcag     3420

gaggcccugg aacacaucgu cgugucuuuc aaacagaacc ugagaguuau uaauaaggcu     3480

accaacuauu acgagaaaug ggaagagaag aauggcgugc uugucaaaaa gaaggugaaa     3540

caagagggga caaacugggc cauccgcaag ccgcugcaua aagaaaccgu uuccggaaag     3600

auugugcucg accggaaaau cggcaaggau aagauucuga cagccacgag aaaaagcguc     3660

gacaucaccu uuacagagaa gauuaucucu agcaucaccg auacagggau ucagaaaauc     3720

cuccugaauu aucuuaagua caaaggaucc ccugaagugg cuuucagcgc cgagggccug     3780

gaggaacuca acaagaauau ugcgcaguau aacgacggga agaaacacca gcccaucuac     3840

aagguucgca uuuuugagga aggcucuaaa uucccccugg gagagaccgg cgcuaaggcc     3900

acgaaauaug uggaagccgc uaaggggacc aaucuguuuu ucggagucua ccaaggcaag     3960

gggaaacgga cauacgccac caucccucuu aacgagguga ucgagagaca gaagcaggga     4020

cugccguccg ugcccgaacg gaaugagaaa ggcgaaccuc uccuguucag ccucuccccc     4080

aacgaucugg ucuauguucc uauggagggg gaaauugcgg agacaaucga cuuuaacaau     4140

cuuucuaagg agcagaaaga acgcauuuac aagaccguga gcuucacagg caaccaaugc     4200

uuuuucgugc aggaggcugu cgccaccccc aucgugaaua aaauugaaua uuccccucug     4260

aacaagaugg agagagacau ccugggaacg augaucaagg aaguuuguau uaaacucaag     4320

guggaucggc ugggcaauau cacaaaagcc uga                                  4353


<210>  4538
<211>  4353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4538
augaagagaa uccugggccu cgaccugggg agcaacucca uuggaugggc cuucguggag       60

caggauuuug aaaauaaaca aggcaagauc auugcuaccg ggucucggau cauucccaug      120

gaccagggaa uccuuggcga uuucgagaaa gggaacacag ucagccagac ggccgaacgc      180

accacauaca gauccaugcg gcgccugaga gagcggcacc uccugcgcag agaacggcuu      240

caucgcguuc ugcacauccu cggauuucug ccuccgcauu augacgcgca gauugauuuc      300

accaagagau acggcaaauu uaucgacaau gcugagccca agauugccua uaacaauggc      360

aacuucaucu uuaugaauag cuucaacgag augauugaag auuuuaagaa auaccaaccu      420

cagcuguucu auaagaaaag caauggggag gaauccaaga uccccuacga cuggacaauu      480

uauuaccucc ggaaaaaggu gcugucucag aagaucaccc agcaagagcu ugccuggcug      540

auccuccacu uuaaccagaa acgcggauac uaucagcuga gaggcgagga agagacagaa      600

aauccuaaca aggagguggc uuuccauagc cuuaaagucg uggauguuga agccgaggcu      660

cccaauaaga aaggggagau uugguacacc auccggcugg aaaacggaug gauuuaucgc      720

agaacgucca agaucccucu cgacgauugg aaaggcaaga cccgggacuu uauugugaca      780

accgaucuga augacgacgg gacaaucaag gucgauaaag agggaaagga aaaacgcagc      840

uucagagccc cggacgagaa cgauuggaac cugcucaaga aaaagaccga acacgacauc      900

gauuucucug gcaagacggu gggcgcguac auuuaugacc aucugcuuca gaaucccaac      960

cagaaaauca aggggaaacu gguucggaca auugagagaa aguacuauaa agaugagcuc     1020

aaggccaucc uggcuaagca gauugcccuu cagccugaac uguuuaccga gacacuccug     1080

gcugacugca uccgcgaacu guaccggaaa aaugagaccc aacagcagaa ccuccugucc     1140

aaggauuucc uucaccuguu uguggaagac aucauuuucu accagagacc ccuccgcagc     1200

aaaaagucua ccaucgccaa uuguacacug gagaaacggu ccuauauuga uaaggagacg     1260

aacgaaagaa aggacaccuu uaucaaaguc ugcgccaaga gcaauccuua cuaucaagag     1320

uuccgcgugc uucaguggcu gcagaaccuc cggauuuacg aaaucgagac agacagagag     1380

gugaccgcgg aauuuauuga gacagcugaa gauuaugagg cccuguucaa uuuucugaug     1440

ucucagaaag aaaucgacaa cgagggacuc cugaaguacu uccuuuuugc uaaaaauccc     1500

caaaucaagg auaaagucgc caaguccgag cugaagaaau ggcucaccac guaucgcugg     1560

aacuacguuu acgacgccgc ugauaauagc uccaagaaau auccgaugaa cgaaacaggc     1620

uacgaccugc agcgguaucu gcagaaggug gaggggcuuc cugcgaauuu ccucaccuuu     1680

gauauuaccu acagacugug gcaucugcuc uauucuguga aagacaaggu cgaguucgaa     1740

aaggcccuga aaaaguucgc ucaggaauac caacuugaug aggccagcuu uguggacucc     1800

uucaaaaacu uuaagcccua uccuaacgag uacggaucuu ucucugaaaa agccaucaag     1860

aagcugcucc cccugaugcg guuuggcaau cacugggauu ucaaccgcau ugacguuucc     1920

acacagaaaa gaaucgauga ccugauuaau gggguggcua acgacgagau ccggaccauc     1980

auuagagaaa aggccgagaa auaccagcuu aauagcgaau cuuguuuuca gggacucccc     2040

cuguggcugg cgcaauaugu cguguacaac cgccacgcug aggccacaga uaucgagaag     2100

uggacgagcg uuuccgaccu cgaaaccuau cuggaggauu ucaaacagca cucucuucgg     2160

aauccuauug uggaacaggu cgugacagag acccugagag ucgugcgcga caucuggcaa     2220

caguacggcg gcgggcaggc caacuuuuuc gaugagauuc auaucgaacu cggaagagag     2280

cugaagaaga cagcugagga acggaaacag cugagcaauc gcaaccaaga aaaugagaac     2340

accaaucuua gaaucaagaa acuccuggcc gagcugaagg aagacgcuuc cauucagaac     2400

guucggccgu auagccccgu gcagcaggag cuccugaaaa ucuacgaaga gggcgucuuu     2460

ucuuccacgg aucaagugga agaggacauu cagaagaucc gcaaaaaggc cgagccuacc     2520

acacccgaaa uucagagaua uaagcugugg cuggagcaga aauacaagag cccuuacacc     2580

ggggaaguua ucucucucac aaaacuguuc accgaggaau augagaucga gcacauuauc     2640

ccccaauccc gguacuauga ugacagccug uccaauaagg ugauuugcga aucugcgguc     2700

aacaaacuua agacgaacca gcucggacug gaguuuaucc agaaucauca cggcgaaaag     2760

gugcgcauua cagggaacaa agaggugacc aucuucacag aggaacaaua caaggaguuc     2820

guuaaaaagc auuaugccaa uaacagcgcu aaacagcaga agcugcuccu gaaugaaauu     2880

ccggagaaga uggucgccag acagcuuaac gauacccggu acaucuccaa auauaucucu     2940

gaaauucuga gcaagaucgu gcgcuccgac gagaaagaug agggcgugaa uagcaagaac     3000

gucauuccuu guaccggaaa aaucacaacg gcucucaagc aagacugggg ccugaaugac     3060

guguggaacg aacugauucu ucccagauuu gagcggauga aucagcugac ccagacacaa     3120

cucuucaccu cuuacaacga uagauuucaa aagaaucugc cuacaguucc caucgaauac     3180

ucccaggggu uccagaaaaa gcgcaucgac caccggcacc augccaugga ugcgcucgug     3240

auugccugcg cuaccagaga gcacaucaac uauaugaaua accagcaugc ccuggacaaa     3300

aacaaggaua aaaaggaaaa gcaacagagc cgcauugacc uucggacgau ccugugugag     3360

aaaaagaaua acaauuggca guuuaaaaag ccuuggucua cauucaccca ggagaccaaa     3420

gaagcucucg agcacauugu cguguccuuu aagcaaaacc ugagaguuau caauaaagcc     3480

acaaacuacu augaaaagug ggaggaaaag aauggagugc uggucaaaaa gaaagugaag     3540

caggagggca ccaacugggc cauccgcaaa ccccuucaca aggagacagu uagcgggaag     3600

auugugcugg aucggaaaau cggaaaggac aaaauucuca cggcuaccag aaaggaaguc     3660

gauaucacau ucaccgagaa aauuaucucu agcauuacag acaccggcau ccagaagauc     3720

cugcucaauu accugaacua uaaggggucc ccggaagugg cguuuagcgc cgagggccuu     3780

gaggaacuga auaaaaacau ugcucaauac aaugauggaa agaaacauca gccuaucuau     3840

aaggugcgca uuuucgagga aggcucuaaa uuuccccucg gggagacggg agccaaggcc     3900

accaaguucg ucgaagcugc caaaggcaca aaccuguucu uugggguuua ccagggaaag     3960

gagaaacggu ccuacgcgac caucccccug aaugagguga uugaaagaca gaagcaaggc     4020

cuuccuagcg ugccggagcg gaacgaaaaa ggggagcccc uucucuucuc ccugucuccu     4080

aaugaccucg ucuaugugcc cauggaaggc gagaucgcug agacaaucga cuuuaacaac     4140

cugagcaagg aacagaaaga gcgcauuuac aagaccguuu ccuucacagg aaaucagugc     4200

uuuuucgugc aggaagccgu cgccaccccu aucgugaaca agauggagua uucuccccuu     4260

aauaaaaugg aaagagauau ucugggcacg augaucaagg agguuuguau uaaacugaag     4320

guggaccggc ucgggaacau cacaaaagcu uga                                  4353


<210>  4539
<211>  4353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4539
augaagcaca uccugggccu cgaccugggg agcaacucca uuggaugggc cuucgugcag       60

caagauuuug agaauaaaca gggcaagauc auugcugccg ggucuagaau cauucccaug      120

gaccagaaaa ugcuuagcaa cuucgaagag ggaaccacag ucucccagac ggcggaacgg      180

accacauacc gcucuaugag acggcugcgc gagagacauc uccugcggcg cgaaagacuu      240

caccggguuc ugcauauccu cggcuuucug ccuccgcacu augaugcuca aaucgacuuc      300

accaagcgcu acgggaaauu uauugauaau gccgagccca agaucgccua uaacaaugga      360

aacuucauuu uuaugaauag cuucaacgag augaucgaag acuuuaagga gcaucagccu      420

cagcuguucu acaaaaaguc caauggcgaa gagucuaaaa uucccuauga uuggacaauc      480

uacuaucuca gaaagaaagc ucugagccag aagauuaccc aacaggagcu ugccuggcug      540

auccuccacu uuaaccagaa gcggggcuac uaccagcugc gcggggaaga ggaaacagag      600

aauccuaaca aagaaguggc uuuccacucc cuuaaggugg ucgacgugga ggccgaggcg      660

cccaauaaaa agggagaaau cugguauacc auuagacugg agaacggcug gaucuaccgg      720

cgcacgagca aaauuccucu cgaugacugg aaggggaaaa ccagagauuu uaucguuaca      780

accgaccuga augaugacgg aacaauuaag cuggacaagg aaggcaaaga gaagcggucu      840

uucagagccc cggaugaaaa cgacuggaac cuccugaaaa agaaaaccga gcaugauauc      900

gacuucuccg ggaagcccgu gggaacguau aucuacgauc accuucugca aaauccuaac      960

cagaagauua aaggcaagcu cguccgcaca aucgagcgga aauauuacaa ggacgaacug     1020

aaagcuauuc uugccaagca gaucgcucug caacccgagc ucuuuaccga aacacugcug     1080

gccgauugca uuagagagcu cuaucgcaag aaugaaaccc agcagcagaa ccugcuuagc     1140

aaagacuucc ugcaucucuu uguggaggau aucaucuucu accaacggcc ucugagaucu     1200

aagaaaucca ccauugcgaa uuguacacuu gagaagcgca gcuacaucga caaagaaacg     1260

aacgagcgga aggauacccc cauuaagguu ugcucuaaau ccaauccgua uuaccaggaa     1320

uuuagagugc ugcaguggcu ccagaaccug cgcaucuaug agauugagac agaccgggaa     1380

gucaccgccg aguucaucga aacagcucaa gacuacgagg cccuguuuaa uuuccucaug     1440

agccagaagg aaauugauaa cgagucccug cuuaaauauu uucuguucgc uaagaauccu     1500

cagaucaaag acaagaccgc caaaaucgag cucaagaagu ggauuacgac auacagaugg     1560

aacuaugugu acgauguggc cgacaauucu agcaaaaagu accccaugaa cgaaaccggc     1620

uaugaucugc agcgcuacau gcaaaaaguc gaggggcugc cuaccaauuu ucuuacauuc     1680

gacaccacau aucggcucug gcaccuucug uacuccguua aggauaaagu ggaauuugag     1740

aaggcucuca agaaauucgc gaagaaaaag cagcuggaca aaacgagcuu cguggauucu     1800

uuuaagaacu ucaagcccua ucccaacgaa uacggauccu uuagcgagaa agccaucaag     1860

aaacuucugc cucucaugag auucggcaau cacugggacu uuaaccggau ugaugucucu     1920

acccagaagc gcaucgacga ucugauuaau gggguggcua acgaggacau cagaacaauc     1980

auucgggaaa aagccgagaa guaucagcug aauagcgaaa ccuguuucca agaccuuccg     2040

cucuggcugg cccaguacgu uguguacaac agacaugcug aggccacaga uaucgaaaag     2100

uggaccuccg ucucugaccu ggaggcguau cucgaggauu uuaaacagca cagccugcgc     2160

aaucccauug uggaacaagu ugugacggag acccuucggg ucgugagaga caucuggcag     2220

caguacggag gcgggcaggc uaacuucuuu gaugaaauuc auaucgagcu gggacgcgag     2280

cucaagaaaa cagccgaaga gagaaagcaa cuguccgaca aaaaucagga aaacgagaau     2340

accaaccugc ggaucaagaa acuucucgcc gaacugaagg aggaugcuag cauucagaau     2400

guccgcccuu auucucuggu gcagcaagag cuccugaaga ucuacgaaga gggcguuuuc     2460

uccagcacag acgaaauuga ggaagauauc cagaaaauua gaaagaaagu ggagcccacc     2520

acgccugaga uccagcggua uaagcugugg cuggaacaga aauacaaguc ucccuauaca     2580

ggcgagguca ucucccucac caagcuguuu acagaagagu acgaaauuga gcacaucauu     2640

ccgcaaagcc gcuacuauga cgauucccug ucuaacaaag ugaucugcga gagcgccguu     2700

aauaaacuua aaaccaacca gcucgggcug gaguucauuc agaaccauca cggagaaaag     2760

gugagaauca ccgcuaauaa agaagucaca auuuuuacgg aggagcaaua caaggaguuc     2820

gugaagaaac acuaugccaa caauuccgcg aagcagcaga aacugcuccu gaacgaaauc     2880

ccugaaaaga ugguggcccg gcagcuuaau gacacccgcu acaucucuaa auauauuagc     2940

gagauccugu ccaagauugu uagaagcgau gaaaaggacg agggcgucaa cucuaaaaau     3000

aucauucccu guacagggaa gaucaccaca gcucucaaac aagacugggg acugaacgau     3060

guguggaaug agcugauccu uccucgguuc gaacgcauga accagcugac ccagacgcag     3120

cucuuuacau ccuacaauga cagacugcaa aagaaccucc ccaccgugcc uauugaguau     3180

agccagggcu uccagaaaaa gcggaucgau cauagacacc augccaugga cgcucugguc     3240

auugccugcg cgacccgcga acacaucaau uacaugaaca aucagcacgc ccuugauaag     3300

ucuaaagaca agaaagagaa gcaacagucc cggauugauc ugagaacaau ccucugugaa     3360

aagaagaaca acaauuggca guuuaaaaag cccuggcgca ccuucacaca ggaggcuaag     3420

gaggcccugg aacauaucgu gguuagcuuu aaacaaaacc ugcgggugau uaauaaggcu     3480

acgaacuacu augagaaaug ggaagagaag aauggggucc uugugaaaaa gaagguuaaa     3540

caggaaggca ccaacugggc caucagaaag ccgcugcacg aggagacauu cucuggaaaa     3600

auuagccuca aucgcgaaaa ggugggcaaa gggaacaucc ugaccgccac acggaaggcu     3660

gucgacucca gcuuugaugu gaagaaaauu ucuaagauca ccgacacggg cauucagaaa     3720

auccuccuga auuaccuuaa guauaaagga uccccugagg uugcguucag cgccgaaggg     3780

cuggaggagc ucaacaagaa uaucgcucaa uacaacaaug gaaagaaaca ucagcccauu     3840

uauaagguga gaaucuacga aaaaggcucc gggcgcuuug uccugggcga gaagggaacc     3900

aaaucuaaga aauaugugca gggcgccccc aaccuguacu ucggggugua ccagggaaag     3960

gaaaagcgga cauaugccac cauuccucuu aaugagguca ucgaaagaca aaaacagggc     4020

cugccgagcg uucccgagcg gaacgagaag ggggaaccuc uccuguuuuc ccucucuccc     4080

aaugaucugg uguacgugcc uauggaggga gaagcuacag agaccauuaa cuucaacaau     4140

cuuaacaaag aacagaagga gcgcaucuau aaaacaguca gcuucaccgg caaucagugc     4200

uuuuucgugc aagaggccgu ugcguccccc aucgugaaca agauggaaua cucuccucug     4260

aauaagaugg agagaagcau ugucgggauc augauuaaag aaguguguau caagcugaaa     4320

guugaccggc ucggcaacau uacgaaggcu uga                                  4353


<210>  4540
<211>  4362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4540
augaagaccg ugcugggccu cgaccugggg acaaacagca ucggaugggc ccuuauucag       60

cacgauuucg acuccaaaaa gggcgagauc cuggggaugg gaucuagaau uauccccaug      120

agccaagaua uucucggcga auuugggaaa ggaaauuccg ucucucagac ggcugaccgg      180

accaaguucc gcagcgccag acggcugcgc gagagacauc uucugcggcg cgaaagacuc      240

caccggguuc ugaacauccu gggcuuucuc ccucgccauu acgcggcuga uaucgacuuc      300

gagaaaagac ugggccaguu uuucgaaggg aaggagccga aacuugccua ugauaauaac      360

caguuuauuu ucacaaaguc cuuuggaaag augcuggccg acuuccggca acaccagccc      420

gauuuucuca aagacgagaa gucuaaugau cugcuuaucc cuuacgacug gagcauuuau      480

uaccugcgca aagaagcucu caccaagaag aucgagaaag aagaacuggc cuggauuauc      540

cugaacuuca aucagaagag aggcuauuac cagcuccggg gagaggaaga ggaagagaac      600

cugaauaaac uuguggaauu ucauucccug aagauuaucg auguggucgc ugacgagaaa      660

cucaacaaua agggagagac augguauagc cugcuucugg aaaacggcug gaccuaccgc      720

agaucuucca aagugccccu cuucgacugg aaggagaagg uucgggauuu caucgugaca      780

accgacauua augaugacgg gagcgucaaa acggauaagg aaggaaacga gaaaagaucu      840

uuucgcgccc cuuccgacga ugacuggacc cugcugaaga aaaagacaga acaagauauc      900

gacaagagcc ggaaacccau uggcgcguac aucuaugaug ccauucucaa gaauccuaaa      960

cagaagauca acggcaaacu ggugagaacc guugagcgca aguucuacaa ggaggaacuu     1020

aaacugaucc uccagaagca gaaagaguuu cacccggaac ugcaaucuac agaccuuuau     1080

aacgagugca uucgggaacu guacaagaau aacgacgcuc accagcucca gcugaauaaa     1140

aaggauuucg ugcaucuguu ucucgaggac aucauuuucu aucaaagacc ccugcgcucc     1200

caaaagagcu ccgucgggaa cuguccucuu gaguuucgga aauucaagga uucugaagga     1260

aucgagaaag uggaauaccu gaagaccauu cccaaaagca auccuuauuu ucaggaguuc     1320

agaaucuggc aguggaugua caaccucucc aucuacaaga aagacgauga cgagaaugug     1380

acgcgcgauu uucugaagac aauugaagac ugggaggauc uguucgaauu ucuuaaccac     1440

cggaaggaca ucgagcagga aacccuccug aaauuccugc ucggcaagaa uggggucaaa     1500

ggaaaggccc ugaaaguuga ggcuggcaag uucagaugga acuaugugag cgauaagauu     1560

uaccccugca augagacaaa agcccuuauc auuucuaagc uggaaaaagu gaaggggguc     1620

gaggaaaaau uucucaccga ggaaaucgag uauaagcugu ggcaucugau uuacuccgug     1680

accgacaagg uugaguauga aaaagcgcuu aaggccuucg cuggaaaaca gaagcucgau     1740

cuggugagcu uugucgacuc uuucaaacgc uuuccgccuu ucaagaacga guacggcgcc     1800

uauagcgaaa aggcuaucaa aaagauccug ccccuccugc ggacaggcaa auccuggaau     1860

uggauggcca uugacucuaa ggugagagau cggaucaaca aaauuaucac gggggaguuu     1920

gacgaagaga uuaagaauaa aguucgcgag aaggccgaaa agcacagccu uaaaaaggag     1980

aacgauuucc agggacugcc ucucuggcug gcucaguacg uggucuacgg cagacacucc     2040

gaagcgagcu uuucugggaa auggaacucc guggacgauc ugaagaaaua ucuugaggag     2100

uucaagcaac auagccuccg gaaucccauc gucgaacagg ugcugaccga gacacugaga     2160

guuguggccg acaucuggca guuuuacgga aagggcgaaa aagauuucuu uucugagauu     2220

cacaucgaac ucgggcgcga gaugaagaac accgcugagg accggaaaga aauguccgcc     2280

auuauccagg ccaaugagac aaccaaccug agaauuaagg cccuucugaa ugaacuccug     2340

caagauaaaa aggucgagaa cgugcgcccc uauagcccuu cccagcagga aauccugaag     2400

aucuacgagg acggcguucu uucuagcgau auugagaucc cggacgauau ucagaaaauc     2460

uccaaggcug cgcaacccac gaaaucugaa cuccagagau auaagcugug gcuggagcag     2520

aaauaccgga gcccuuauac aggagcuaug auuccccucg gcaagcuguu caccuccgaa     2580

uacgagaucg aacauaucau uccucaaagc cuuuacuucg acgacucuuu uuccaauaag     2640

gugaucugug agagcgccgu caacaaacug aaggauucuc gccucgggau ggaguucauu     2700

aaagaaugcc acggaauggu gguggagacc ggcuuuggga aguccguuac agucuucgaa     2760

gaggagaccu auagagacuu uguggugcag aauuacagca aaaaccauuc uaaaaagagc     2820

aagcugcugc uugaagagau ccccgaaaag augauugagc ggcagaugaa ugauacacgc     2880

uauaucucca aauucauuag cucuguccug uccaacaucg ugagagaaga gguuaaugac     2940

gauggaguga acagcaagaa uaucgucccg ggcaacggga aaauuacgac ccagcucaag     3000

caagacuggg gccugaacga uaucuggaau gagcucauuc ugccucgguu ugaacgccuu     3060

aacgugcuga cagacuccaa acacuucacc gccuggucug agaaucacca gagacuccug     3120

cccacaguuc cuaucggacu gagcaagggc uuuuccaaga aacggauuga ucaucgccac     3180

caugcucuug acgcccuggu gaucgcuugu gccucuagaa accacaucaa uuuccucaac     3240

aaugcgcacg ccauugauaa gaaaaagaac agcgaagaga aacagaaguu ucggcaugac     3300

cugaaggcua uccucugcga caaaaaguac uccgauaaau cugaaaagaa uuauagaugg     3360

auuuucaaaa agcccuggga caacuucacc aucgauagca agaaugcccu ggacaaaauu     3420

aucgucuccu uuaagcagaa ccuucgcauc auuaauaaag cuacgaaccg guacgagaag     3480

uggguggaua gagacgggau caaaguuaag gaguggcaca agcaagaagg aauuaauugg     3540

gccacccgca aaccucugca uaaggauaca gugagcggca aagucgaccu caagcgggug     3600

accgugcccg aggggaaaau ccugacagcg accagaaagu cucuggauac guccuucgac     3660

cuuaaaguca uugaaagcau cacagauacc gggauucaga agauccugaa gaacuaucuc     3720

acaucuaaau ccaacaaucc ggagcuggcc uuuagcucug aaggcguuga ggacaugaac     3780

aagaauaucc gcaaauacaa cgaugggaag cuccaccagc cuauuuacaa agugcggauc     3840

uucgagcugg gcuccaaguu uccccuugga caguucggca auaagaaauu uaaguaugug     3900

gaaaccgcua aagggaccaa ccuguucuuu gccgucuacg aggacgaaaa uaagaacaga     3960

aauuaugaga caauuccccu gaacgugguu aucgagcgcc aaaaacaggg acucagcucc     4020

gugccuauua agagcgaaaa gggccauaaa cugcuuuucu accugucucc gaaugacauc     4080

gucuaugugc ccgggagcaa cgaggaauuu uccaucgauc ggcucuacag auucacggac     4140

ucuagcgaua agaccgcuaa uuuuauuccu cuguccguuu cuagccucau cuucuccucu     4200

aacaaaaaug agcagaagaa aauuggaauc agcuauccca uucaagacga guucggccug     4260

ggguccccuc agagcaagaa ccagaagucu aucgauggca ucaugauuaa agagaagugu     4320

gugaaaguca auaucgaccg gcuuggacgc guguccuuuu ga                        4362


<210>  4541
<211>  4368
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4541
auggccaaga uccugggccu cgaccugggg accaacagca uuggaugggc uguggucgag       60

gaucagaaua acaaaacaac gcuuaucgac aagggcguua gaauuuucuc cgaaggggug      120

aaaaucgaga agggaauuga aaaaucuaag gccgcggagc ggaccgauuu ucgcagcgcu      180

agaaagauca aauuccggcg cagacugcgg aagcaagaaa cacucaaagu gcugaucgag      240

aauaacaugu gcccccuuac cauugaggaa uggacaaagu ggaaaaagga caagaaagag      300

uacccuaaga auccggauuu uaucaacugg cugaaaaccg acgaacucaa gaauauuaac      360

cccuauuucu uucgcgauaa agccuccaga acagagcugc cucugaugca gaccggccgg      420

gcccucuacc accuggcuca gcgcagaggg uuccuuucua aucggcugga ccagggaagc      480

gauggcuuua cggagcaaau ccgccucgac cugauuaaga aaaucgaaug ugagaccgau      540

aagacagaac uucugaccuc cguccagucu auuaucgaca cauauagcga ggaagaggau      600

aaggacgcca aaaagcuccu gaaaggcgug gagaagcugu gcaaaaucaa caaugaacuc      660

cccauuaccg aguucaagga aaagcugauc aacuaccuua auaaagcuga caaccugggg      720

gccguuaaga aaggaauugc ggaugugucc aagaaaauca cggagacagg cugugaaacc      780

cucgggcagu auuuuuacaa gcuguaucag aaucaaagca agauuagaaa agucuacaca      840

gcccggaccg agcauuacua ucacgaguuc cuuaagaucu gcgaaaccca gaacaucugu      900

gaccagcuga aacauaagcu cgauaaagcu auuuucuacc agcgcccucu gaagucucaa      960

aagggacuga ucggcaaaug cguguucgag aagaacaaaa caagaugugc cguuucccac     1020

cccgcuuaug aagaguaccg ggcccuccag uuuauuaaua gcaucaaggu gucugaaacg     1080

aaagacggga agaaagauuu ccuguccuuc gacgagagaa agaagauuug gccuaaauuu     1140

auccgcaaga gcaaaccgaa cuucgauuuu aaggagaucg cggaaacccu ugacaaaaag     1200

ggacugucuc gguauuacaa uuauacaggc cauaccuccg ucagcggcug ccccacaauu     1260

gcccagcuca ccaacaucuu cggggagaau uggcaagaaa cgcuguuuga gaaguacaca     1320

aacaaacaga auaccaaggg acuuaaaacc auugaugagg uggugacaga caucuggcac     1380

guccuguuca ccuuugauaa ggacgaaaag auuaucgagu ucggcaaaca gaagcucggg     1440

cugaacgaag uucuggcuaa aaaguuugga gccauuaauc ucaaaaagga cuacgcuucc     1500

cugucucuua aagccaucaa caagauccug ccuuaucuca gagagggccu gauuuacagc     1560

uaugccgugu uccuggcuaa uaucgaaaac guggucgggg aggagauuug gaagaaugau     1620

gaaaacaaaa aucuuaucaa gaaagagcuc caucugcuga uugacaacuu uaacgaaauc     1680

aagggacucg aggcgguggu uaaugauuuc aucgccgacu uuaaaaagga auacaacaau     1740

gcucacaaag auuaugugcu ggacgagcag gauaagaaga uucuucugga gaaaaucgaa     1800

uccaacuacg gcaagaauaa auucgagagc cucgaacccg acaagaaaac agagauuauc     1860

aacucuacgg aaaagauggu cgccgagcaa cugcgcaagc agcggggcga guucauuaaa     1920

ccuaagagac uggaugagaa aaucuccgaa uuucuuauca auaacuucaa ugugaccaag     1980

aaaagcauug cccgccucua ucaccccucu gacguugaaa ucuuuaagac agcuagccgg     2040

aacuccgagg ggcugcugcu ccugggaucu ccgauuguga gcuccaucaa gaauccuaug     2100

gccaugagag cgaugcauca gcuucgcaaa cuggucaaca cccucauuaa ggagaauauc     2160

aucgaugaag agacaaaaau uaacaucgaa cuggcucggg agcugaauga cgccaacaag     2220

agagccgcua uuaaaaagug gcaggaugaa cgccaaaagg cccgggagga guacaaaaag     2280

agcaucaaag aacuuuucaa ggcugagugu ggcaaagaca uugaacccuc ugacuccgag     2340

auccucaagu acgaacugug ggaggagcag cagcacauuu gcaucuauac cgggaagacg     2400

aucaacauuu gugauuuuau cggagccaau ccuaacuucg acauugaaca caccaucccc     2460

aaaagccugu gcuuugauaa uucuaacgag aaucucacac ugugcgacau ucaguacaac     2520

agagauguga agcggaauaa aauccccacc gaacuuuccg acuauaacag caucaagccu     2580

cgccuggaua aauggcucgu cuucauugac gauuacgaga agcuguauaa uacacggaaa     2640

aaggcgaagg gcaucgaaac caaagagcaa aaggacaaaa gaauuaagga gaaacauuac     2700

uauaagcugc accuugauua cuggaagggg aaauacaaga gauuuaucau gguggacguu     2760

ccguccggcu ucaaaaacuc ucagauugug gauacgggaa ucaucacaaa guaugccauu     2820

agcuacauga aauccaucuu ucccaagguc uauucuguga agggcaauau uguugcugaa     2880

uuucgcaaaa ucugggggcu ccagaacgac uacgagaaga aagaacgggu gaaucauguc     2940

caccauugua uugacgccau caccauugcu ugcaugagca agcagaaaua ugauacacug     3000

gccaagaucu accacgagga cgaggcggga uuucacaaga ucaaaaagga aacccaacug     3060

guguccaaac cuuggaccag cuucacaaug gaugugcuca acauugagaa ggaaaucuuc     3120

guuucucauc acacgcccga caauaugaaa aagcagucca aaaaggucgu gagagagcgc     3180

ggcauuaucg ccccuaaggu gauuuaugaa accgaugcua acaacaauac acugagagac     3240

aaauacggga agaaaaucgu caaggaguac auccuucuga aagaugugaa cggaaaucuc     3300

auucccauca agggcaagcg gcugaccccg agcgacauua ucaacaaagu ugaugggauu     3360

gacuauuuuu cucugaucau caaugauaag auucaguacu augaguucgc caaauccaag     3420

aaaacaggca agaucauuua caagaaagaa ccuaucuauc uucaaggaga caccgugcgc     3480

ggcagccugc aucucgacac guuuuacggg gcuauugcca aggauaucaa cggaaauauu     3540

aucacaaaca ccaauggcaa aaucgccccc aacuauguca uuagaaagga gcugucuaaa     3600

cucaaggaaa ccgacaucga gaauauugug gauccuguug ugcgggggaa gaucauugaa     3660

gcuaaaaaga acaaacugau cacauucaau aagagcggag cggucaucaa aaccacaauu     3720

uggaugaagg agaaccagau ccccauuaau aaagugcgcg uuuacuccag ccuuaagaac     3780

ccucugcccg acuuuaagaa acacagagau cucucucacc ugacguacaa gcagcaguau     3840

aaugugucca acgaggaaaa cuacugucug gccaucuaug agggcaaaaa uaagaaaggg     3900

aacgucugcc ggaccuguga acuugugaau augauugacg cuggcaacua cuaucgccug     3960

agcaauaaga acuggaagca acaguacccg aucgugccug agaaagauuc cgucacaaau     4020

uauucucuca agaccaucau uacaaaagga cugaugguuc uccuguacga aaagagcccc     4080

gaggagaucu gggaacuuac cuacaaccag cagauggaga gacuguauga aauuacgcaa     4140

cucgacaccg agaaauccgc cgagaucaag cugcuguucc aucaggaagc ccgggagaag     4200

aaagugauua cagaauacau gggccuuaag accgggauga aaggaggcaa gaauaucggg     4260

aaacacaagg aguuucccug gauuaaggug ggaccuaacu cuuucgaugc ucugaucgaa     4320

ggcaucgacu uuaaacucac accgaccggg aagauuauca aacuguga                  4368


<210>  4542
<211>  4374
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4542
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc caaagugucc       60

gucgaugacu acggcaauua ucuucacgau aucaagcugg ggucuagaau uauccccaug      120

agccaggacg uucucggaaa cuucgauucc ggcgugacgg ugucucaaac cgcucagcgg      180

acagaguuuc gcagcaaaag acggcugguc cagcgcaauc uucagagacg ggaacgccug      240

cauagagugc uccacguucu ggacuuccug ccugcccauu acgaugcggc uauugggugg      300

gaccggaccg aggccgugac auauggaaag uuucucaacc acgccgaacc gaaacuggcu      360

ugggaggaac uuggcaaugg caagcgcaga uuccuguuua uggauuccuu ccaugagaug      420

gucgccgacu uugcucggug ccaacccgcc cucguggcgg augggaagaa aaucccucug      480

gacuggacca ucuacuaucu ucgcaagaaa gcccugacac aggcuauuac caaggaggaa      540

cucgccugga uccugcugaa cuucaaucag aaaagaggau acuaucagcu ccggggcgag      600

gaagaggagg aaaaccccac gaagcaagag gaauacuacg agcugaaggu ugugucuguc      660

gaagcuaccg auagccgcaa agggaaugac acaugguaua acgugcaccu ugagaaugga      720

uggauuuaca gacgguccag caagaucucu cuggacgauu gggugggcaa agucaagcag      780

uuuauuguua ccacagagua ugaaaaagac ggggccaccc ucaagacgga uaaagaggga      840

uacgugaaga gauccuuccg cgcgccuaac gaaaaugacu ggggccugcg gaagaaaaga      900

acagagaccc uucuggaaau gagcggcaag acaguggggg ccuuuaucua ugaucaccuc      960

cugcaggagc ccgcugacaa aauucgcgga aacuucaucc ggaccaucga gagacauuac     1020

uauaaggccg aacuggaggc uauucucgcc gaacagugua aauaccacau cgagcugcgc     1080

gauaaggaaa ugcuugaggc cugcgcucgg gagcuguacc ucaauaaccu gccucaucaa     1140

agaucucuua ugaagaaaga caugaccuau cugcuccugu acgaucugau cuuuuaucag     1200

cgcccgcuca aguccaaaaa ggcgcugauu gccaacuguc ccuacgaaua uuacgaguau     1260

gucgacaaag aaacaggcga gauuaagcgg cagaauauca agugcaucgc uaaaagcaac     1320

ccuuacuucc aagaguucag acuuuggcag uuugugcuga aucuccgccu guucgaucgg     1380

guugaagaca aggaggugac ggccaacuac cugcccaccg ccgacgauca cgcuagacuu     1440

uuuacauauc ucaaugaccg gaaagaaauu aaccaggaga cccugcugaa ggauuacuuc     1500

aaacucaaga aggucaaaau cgggaaugaa aaggaguauc cuauucgcug gaacuacauc     1560

gaggacaaag aaaagaaaua ucccuguaau gagacaagau acgaacugcu uauggcccug     1620

gaucgggcgg gaauugagca caaguggcuc gacgauaaca aucuggaaua uagacugugg     1680

caucuucucu acucugugga gguuaaaucc gagagcgaaa ccgcucugcg caagcugaag     1740

aacgacgaug ccuuugugaa uagcuuccuc aaagucaagc cguuuaaaaa ggaguacggc     1800

gccuauucug aaaaagcuau caagcggcug cuugccguga ugagaaaggg guuccugugg     1860

aacgagcagg acaucugcga ugaaacgcgc aaaagaauug agaucauuau caagggagac     1920

gucgaugaga aagugaagga acggaugggc cacgcugccu acagcuuuga ccgcguuucc     1980

gacuuccaag gccucccuga guggcuggcg uguuaugugg ucuacgggag acauagcgaa     2040

gccauggaua uucagcacug ggacucuccc gagcggcuga uggcuuauau caauaacuuu     2100

aaacaguacu cccuucgcaa cccuauugug gagcagugca uccucgaaac acugagaacc     2160

guucaugaua ucuggaagga gaccggacaa auugacgaaa uccacgugga gcugggccgg     2220

gaaaugaagu ccacaucuga ggagcgcgcc agacggaccc agaauguccu ccgcaacgaa     2280

aauacaaacc ugagaauuaa aagccuucug aucgagcuca agaaugauac ggacauuugu     2340

aacgugcacc ccuauucccc caugcagcag gaaauccugc ggaucuacga ggaaggggcu     2400

cugcaagagc uuaauagaga ggaugaagac uucgaggaaa uuucuaaaau cagccagaug     2460

ucccagccua gcggaaagga gcucaaccgc uacaaacugu ggcuggaaca gaaguaucgg     2520

ucuccguaca ccggcaaggc cauuucccuc gcgaaacugu uuacacccgc cuaucaaauc     2580

gagcauguga uuccucagag cagauacuuc gaugacucuu ucaccaauaa gguuaucugc     2640

gaggcugaag ucaacacacu uaaauccaau cugcucgggu augaguuuau caagaaacac     2700

ggcggagaaa uuauccauug uaccacgcug ggcgaugugc gcauuuucag cgaguccgag     2760

uacaaggccu uugugaugga ccacuaugcu aacaauaaau cuaagaagga uaaacugcuu     2820

auggaagaca ucccccagga guuccugagc cggcaaauga acgacuccag auacauuucu     2880

aaggucguga aaacccuccu gagcaauauc guucgcaugg ccgaugaauc cgaggccaca     2940

agcaagucug ugaucgucug caccggggga gugacagacc ggcucaaaaa ggauuggggc     3000

cugaacgacg uuuggaacuc cauuguguac agcagauuug aacgccuuaa ucggcugacc     3060

gggacggagg cuuucggaca cugggagaac aaggauggca aaagagucuu ucagacaucu     3120

augccucucg aacugcagcg gggguuccgc aagaaaagaa ucgaccaucg gcaccaugcg     3180

auggaugccc uggugauugc uugcgccucc cgcaauaucg uuaacuaucu uaauaacgag     3240

agcgccaaua accccaagaa aagagaagac cugcggagac agcucuguga uaagaaucgc     3300

gugauucgga agccguggga gaccuuuaca caagacgcuu acaacgcccu ggaaaauguc     3360

guggugucuu ucaaaaacua ugucagaguu aucaauaagg cgaccaaccg cuacgagcac     3420

uaugaugaga auggcaaaaa gcgguuugug cagcagaaag gaagcgaacu cugggcuauu     3480

agaaagccua ugcauaagga gaccuacuau ggccacguga accugcgccg gaaagaacuu     3540

gucacacugc agaaggcccu cgacgacauc cccuccaucu gcgauaaaaa gcugagagac     3600

uacauuagcg cccuggugga gaaacacuuc aacaagcgcc aacuucuggc ucauuuuaag     3660

ucuacggauu acuauuggca gaaacaguac guuaauaagg uguaugucug gaccuucucc     3720

gacgauauca aaccucucgu ggccacaaga aagcugcucg acaccagcuu cgaugcgaaa     3780

cggauuggga caaucaccga cacgggcauu cagaagaucc ugcgcaacua ccuugaagcu     3840

aaaggaggga augccacacu ggccuuuacc cccgagggaa ucgcugagau gaaccaaaau     3900

auuuccaagu auaacgaugg caagccucac cagcccaucc ugaaaguuag aauuaccgaa     3960

ccgauggggg ccaaguaccg ggugggccag acaggaaaua aaauggcuaa guaugucgag     4020

gcccaaucug gcaccaaccu cuacuucgcg aucuacgaag acagcgaggg gaaacgcucc     4080

uauacaacgg ugccucugga ugagguugcc gaaagacuua agcagcggcu gucucccgug     4140

cccgagaagg acgaaaauga ugucccucuc aaauuuuacc ugagcccgaa cgaccucgug     4200

uaugugccca ccgaggagga acugcagcuu agagagugua agcugaauaa agaccgcauu     4260

uacaagaugg ucuccucuac aggaaacgaa ugccauuuca ucccuuauua cguugcuaau     4320

gugaucuaua acaaaggcgg guuuaauaug cagaugcacc ggccccaaag cuga           4374


<210>  4543
<211>  4374
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4543
auggccaaga uccugggccu cgaccugggg accaacagca uuggaugggc uaucguggag       60

aaaaauaacu ccgauuucac acuuauugac aagggcguca gaaucuuuuc ugaagggguu      120

aaaacggaga agggaauuga acggagccgc gccgcggaga gaaccggcua ccgguccgcu      180

cgcaaaauca aguauagacg gaagcugcgc aaauacgaaa cacucaaggu gcugucuaaa      240

aauagaaugu gcccccuuca gaucgaggau guggaggccu ggaagaaaag cggguucaag      300

gacuauccuc ugauuccgga auuucuccaa uggcugcgga ccaacgauca cgagaauguc      360

aaccccuacu ucuuucgcga caaggccucc agagcuaaag ugucucugug ggaacucgga      420

cgggccuucu aucauaucgc ucagcgcaga ggcuuucuga gcaaucggcu ugaucagucc      480

agcgagggcg uucuggagga acacaacccu cagauucaau cucucaucga ggaccuggaa      540

gagacaucca ccauucuuaa ugaagugcgc gauuacuaua ucaaccuggg gcuccuggac      600

ggagucaagg gcgauuucaa aaaggagcug gacgaggggg agaaaaagcu caaaagccug      660

uacaauacac uuauugccau caucaagaag aacgaaaaug augugcagaa auguaaggac      720

gaaauuauca aaagacugaa caagaaagag gaccucggaa agguuaaggg caaaauuaag      780

gauaucucuc aggcgaugcu ggacgggaau uuuaaaaccc uuggacagua cuucuauucc      840

cuguacaaca agggcaaaau ucggaaccaa uauacgagca gagaggaaca uuaccuccag      900

gaguuuauca ccaucugcaa ggugcagggc auuaauggga ucaacgauga agagauucug      960

cccgaaaaga aauucacagg acuggccaag gaccucuaua aagcuaucuu uuuccagcgc     1020

ccucugaagu cucaaaaagg ccuuauuggg aaguguuccu ucgagaagaa uaaaagccgg     1080

ugcgccaucu cccaccccga uuuugaagag uacagaaugu ggaacuaucu gaauaccauc     1140

aaguucggaa cacagucuga caaaaagcuc cgcuuucuga cccaggagga aaaacuuaag     1200

cugauuccua aauucuaccg gaagaacgau uuuaauuucg agguccucgc uaaggaacug     1260

aucgacaaag gcgccacguu uggguucuac aagagcuccc ggaaaaacga guuucuguau     1320

ugguucaauu acaagccgac agauaccgug agcgcguguc aauuuucugc cucccucaaa     1380

aacgcuauug gagaggacug gcagacaaag guguucaccu auaagacccu gaauagcgag     1440

aaaaaggaag ucauccgcac aguugauuac cgggaccuuu ggcaucugcu cucugccagc     1500

acguccgaca uuuaucugua cgaauaugcu aaagagaagc ugggccuuga agauaaaucu     1560

gccaagaccu uuagcaagau caaacucaag aaagacuucg ccucccugag ccugucugcu     1620

auuaacaaga uccuccccua caucaaagag ggccugcuuu acucccacgc gguguucaug     1680

gccaauauug agaagguggu cgaugaacac aucuggaagg acgugaaaga gagaacaauu     1740

auccagaacg aaauuagcaa gaucaucaaa ucugauguuu uugagcgcga agugauuaau     1800

aucauuaacg ggcugaucaa gaacuccaaa accgagggag agaauuaugc ugaagagagc     1860

aagaacauuu ucaaaacaga auuugacaau gccaucgcca agaucuucga gaagaacaau     1920

gauauugaau uuaaccagcc uuuccucgac gauaccuacc ugaaauaugu cgaggagcug     1980

aagaaauuug acaaggcucg guccuuccuu cccgugccua gacucgauga aaaaguuauc     2040

caauuucugc ugggcaauaa caaggacggg augguguacu gcucugauaa gaauaaaacg     2100

aagaaacucu aucaucccag cgacauugag aucuucaaga aaaagauuau caaggacgaa     2160

uuuggaaacg agaaaauggu ccugggcucc ccgcuuacaa ccucuaucaa gaauccuaug     2220

gccaugcgcg cgcugcacca gcuccggaaa gugcugaaca cccugauucu ugaggaacag     2280

aucgaugaga auacacggau ucauaucgaa auggcuagag agcucaacga cgccaauaag     2340

cgcaaaggga uucaggauua ccaaaacgaa aacaagaagu ucagagagga cgccaucaaa     2400

gagauuaaga aacuguauuu ugaagagugu aagaaagaag ucgagcccac cgaagaggau     2460

gugcugcggu accagcucug gaucgagcag gacaagaagg aaaucuacga gcagggcaaa     2520

agcauuagaa ucuccgauau uaucggaagc aauccugcuu augacauuga acacacaguu     2580

ccccgcucua ucucccaaaa caauagccug augaacaaga cgcuuugcac ccccagauuc     2640

aauagagaga ucaaaaagca gaaaaugccu auugaacugu cuaacuacga ggagauccuc     2700

ccgcgcauug aacacuggaa gaaagaggcc gaagaucuga caagacagau cgaguccauu     2760

agcaagucua ucaagagcgc uuccaccaaa gaggccaagg acaaaaauau ccggcgcaga     2820

cauuaucuga cacuuaagcg ggauuaccuc aaaggcaagu augaccgcuu cauuugggag     2880

gaacccaagg ugggguuuaa aaacagccag aucccugaua ccggaauuau cacgaaguac     2940

gcgcaagccu aucugaaauc uuacuucaag agagucgaau ccgugaaagg cgggaugguu     3000

gcugaguuuc ggaagcagug gggcauucag gaaagcuaca ucgacgagaa uggauucaag     3060

cacuauaaag agaaggaccg cuccaaacau acccaccaua caauugaugc caucaccauc     3120

gcuuguauga caaaggacaa auacgaugug cuggcccaag cguggaccuc ugaagacgag     3180

caggauaagc agaaggccaa aacgcucaug agcgaagcua agcccuggaa aacauuuaag     3240

gaggacauug agaaaaucga agaggaaauu cuggucuccc acuucacccu ugauaacgug     3300

aagaaacagu cuaagaagau cgugagaguu cgggggaaaa agcaauaugu cgccgagauu     3360

gaacgcgacg ugaauggcaa agcuauggcc aagaaagaug ccaacggaaa gaucaucuac     3420

aagcugaaug agaaaggcga aaagauuccu agacuccagc agggggacac aauccgggga     3480

agccugcacc aggauuccau uuauggcgcu aucaaaaacc cccugaauca agaggagauu     3540

aaguacguga ucagaaaaga ccuugaaucu cugaaggcga acgacgucga uaacaucgug     3600

gacgagaccg uuaagcagaa agugaaggaa gccauugaga auaaaauccu ccugcucagc     3660

uccaacgcuc agcagaagaa uaaacugguc gaugacgugu ggcuuaacaa ggagaagcaa     3720

guuccgauua aaaaggugcg caucuaugcc aauagcguca aaaacccucu ggaaauuaag     3780

gagcagucuc uccuguccaa aagccggaag gaacauaaac agaaggugua cgggcaaaau     3840

gaugagaacu acgccauggc uaucuaugaa cuggacggaa agagagaguu ugagcuuauu     3900

aauaacuuca aucuggccaa acucaucaag cagaaagaag gcuuuuaccc ccugaacaag     3960

gagaaagaag uuaaggggaa gaaagugcuc uauccuaucg cgaagucuaa uuaccgcgau     4020

gucgugcuga aacggggcca acaggugguc uucuaugaca aggagguuga aaaccccaaa     4080

gauauuuccg aggugaucga cuuuaaggga agaauuuaca ucauugaggg ccuuagcauc     4140

cagcgcaucg ugcggccuuc ugggaagauu gaugaauaug gagucaucau gcugaaauac     4200

uucaaggagg cuagaaaagc cgacgaaauu aagaaagaua auuacaagcc cgacggcgug     4260

uucaagcucg gggagaaccg cccgacccgg aaaaugaauc acucccaguu uacagccuuc     4320

guugaaggaa ucgacuuuaa ggugcugccu acgggcaaau ucgauaagau uuga           4374


<210>  4544
<211>  4377
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4544
augaccaaga caauccuggg ccucgaccug gggacgaaca gcauuggaug ggcccuuauc       60

aaucaggauu ucgacaacaa aaagggcgag auucugggga ugggauccag aaucauuccc      120

augacccaag auaucaaaga cgaauuuggc aagggaaauu cuaucagcca gacagcugag      180

cggacccgcc ucagaggggu gcggcgccug auucacagaa cacuucugcg gcgcgaaaga      240

cuccaucggg uccugaacau cauuggcuuc cugccugagc acuacgccaa ucagaucgau      300

uuugaaaaac gcuucggcaa guuuaagccg gaguccgagc ccaaaauuuc uuucgacggg      360

aacgauguuu uucucuucga aaagagcuau caggagaugc ugguggacuu uaaaauccau      420

caaccucagc uuguguccaa uggaaagaaa auuccccacg auuggaccau cuacuaucug      480

agaaagaagg cgcucucuaa aaagaucgaa aaagaggagc uggcuuggau ucuucugaac      540

uucaaucaga agcggggcua cuaucagcuc cgcggggaag aggaagagga aacaccuaac      600

aaacuggucg aguuucauag ccugaagauc guggagguua augccgacga accccaaaaa      660

ggaaagccug agauuuggua cucccucgug cuggaaaacg gcugggucua uagacgggcc      720

agcaagaccc cgcuuuucga uuggaaagac aagauccgcg aguuuauugu gacgaccgaa      780

aucaauaacg augggacagu uaaaaccgac aaggagggaa cagagaaaag aucuuuccgg      840

gcucccaagc cugaagacug gacccugcag aagaaaaaga cggaguucga acucuccaaa      900

agcggcaaug aggugggcgc cuuuaucuac gaaucuauuc ugcagaagcc caaccagaaa      960

aucagaggga aacuuauuuc cacaaucgag cgcaaguucu acaaagagga acugaagacc     1020

auucucaaaa cacaacuguu uuuccacaag gagcugaaag augaaaagcu cuauaacgcu     1080

ugcaucgagg agcuguacaa gaauaacgaa gcccaccgga gccuucuguc uaauaaagga     1140

uuugagcauc ucuucaucaa cgacauucug uuuuaucaga gaccucuucg cuccaagaaa     1200

agccagaucu ccaauugucc ccuggaaaag cggaccuaca aaaaggaggg cauugaaauc     1260

accgagggga uuaaagucau cucuaagagc aacccgauuu aucaagaguu cagacucugg     1320

caguggaucu ccaaucugag ccuguacugc aucgaaccua cagagacgaa cgugaccucu     1380

acauuucuca auuccauuga agauuaugag aaccuguucg aauuucuuaa uaaccgcaag     1440

gagaucgagc agaaacaucu gcucaaguac cugcuggaaa aucagggauu caaaggcaaa     1500

cuucucacca acgagcugga aaaguuucgg uggaauuucg uggcggacaa gaaguacccc     1560

uguaacgaga cagggagccu gcuccacacc agacugucua aagucaagaa cauuagcccu     1620

gauuuccuua cgaaagaaau cgagcaccaa cuguggcaca uuaucuauuc cguuacagac     1680

aagaucgagu acgaacaggc ccucaaaacc uuugcucgca agaauaaccu ggauguggac     1740

ucuuucuuug agcauuucaa gaaaauuccu cccuuugaaa gcaccuaugg agccuacucc     1800

gagaaggcua ucaaaaagcu gcuuccccuc auucggcugg gcaaauauug gaauugggag     1860

gccaucgaua gcauuucuaa ggacagaauc uccaagaucc ugagcggcga auacgaugag     1920

aacauuaaaa aucgcgugcg ggaaaaggcg guccaccuca caucugagaa caauuuccag     1980

gggcugcagg aauggcuugc caaauauauc guguacgaca gacauuccga gggaaacgac     2040

cugggcaagu ggaccagcgu uuccgaucuc gagacauacc ugaaagaauu uaagcaacac     2100

ucucugcgga auccgauugu ggagcagguc aucacggaaa cccuucgcgu gguuaaagac     2160

auuuggauca agcaugggaa gggaacagag aacuucuuug augaaaucca cguggagcuc     2220

ggcagagaga ugaaaaauaa cagcgaagac cggaagagac ugaccaauac aauuaccgag     2280

aacgaaaaua cgaaccugcg caucaaagcu cuccugaugg agaugaugaa cgauaaugac     2340

gucgaaaacg ugcggcccua uuccccuucu cagcaggaga uucuuaagau cuacgaggau     2400

ggggcccuga auagcaacau ugaacucgac gaugagaucg ucaaaauuuc caagaaggcu     2460

gaacccacca aaagcgagcu gcaaagauau aagcuguggc uugagcagaa auaccgcucu     2520

ccuuauacag gccaggugau cccccucaau aagcuguuca ccuccgaaua cgagaucgaa     2580

cacguugugc cgcagagcag auuuuucgac gauucuuuuu ccaacaaagu cauuugcgag     2640

agcgccguga auaagcggaa ggacaaccaa cugggacucc aguucaucaa aaaucauucu     2700

ggcgaaaagg uugagcuggg guucggaaaa gugguccagg uguuuacaga ggaacaauau     2760

cuugauuucg ugaaggagca cuacagcaaa aaccgcucca agcauaauaa gcugcuccug     2820

gaagagauuc cugaaaagau gaucgagaga cagcugaacg acacccggua cauuagcaag     2880

uuuguuucuu ccauccuuag caauaucguc cgcuccgaga aagacgauga cggccugaac     2940

ucuaagaaua uucuccccgg gaacggaaaa aucacgacag aacugaagag agauuggggc     3000

cucaacgacg uguggaauga ucugauucuu ccucgguucg agcgcaugaa caccaucaca     3060

aauagcgacc uguuuaccac cuauaacgau aaguaccaga aacaccuccc cacagugccu     3120

uucgaauauu ccaagggguu ucagaaaaag agaauugacc accggcauca cgccauggau     3180

gcucugguca ucgcgugugc cacgcgcgac caucugaauc uuaugaacaa ucaaucugcu     3240

aaaagcgagc ugaagagaua cgaucuccag aacaaacugc ggaagaagga acccuauuuc     3300

aaucagaaag agaacaagca gaaagaggcc uuuaaggacu ucaucaaacc guggggcacc     3360

uuuacagaag auuccaagaa ugcccucgag aagauuauca uuucuuucaa acaaaaccug     3420

agagugauca auaaggcuac caacagcuac gaauccuaua aagacgagga cggaaaucuu     3480

cgcauuggca aggaugggaa accugagaag ggacugauca agcagaaagg ccucaacugg     3540

gccauucgga agccccugca caaagacaca guuagcgggc agaucaaucu gucuagaauc     3600

aaacuuccca acggaaagau ucugaccgcg acgcgcaaga accucgauac auccuucgac     3660

cugaagacca ucgaaaauag cauuaccgau acaggcaucc agaaaauucu caagaacuac     3720

cugcuuucua aagagucccc ugaacuggcu uuuagcccgg aggggcugga agagaugaau     3780

aacgagaucg aaaaguacaa uaacggcaaa uuccaucacc ccaucaauaa ggcccggauu     3840

uaugagcucg gaucuaaauu uaacgugggc caaaccggga auagacgcga caaguucguc     3900

gaagccgcua agggaacaaa ccuguuuuuc gccaucuacc aggaugagaa uaaaaaccgg     3960

uccuaugaaa cgauuccucu uaaugaggug aucgagcacc agaaguggag agcuagccug     4020

cccaaagagg agcaagaaaa gauuccucuc guucccguga acaaacugaa gggcaccuuu     4080

aucuucuccc ucagcccuaa ugaccugguc uacgugcccu cuaacgauga gcuugaacgc     4140

agcgccucca ucgacuuuuc uaagcugaaa aaggagcaga uuaaccggcu guauaaaaug     4200

guuagcuccu cugggagcca gugcuucuuu gugaaguccg aggucgcgac aucuguggug     4260

aauaaaaugg aauacagcuc ccucaacaag auggaaaaga gcaucgauaa ucugaugguc     4320

aaagaaauuu guaucaaacu uaagauugac agacugggaa acaucucuaa ggccuga        4377


<210>  4545
<211>  4377
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4545
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuaucgag       60

caggauuucg aauccaaggc uggcaaaauu ucuggggccg gaagcagaau cauucccaug      120

ucccaagaca uccugggcac auuugagacg gggcagucuu acagccagac cgcggaucgg      180

acacacuauc gcuccaucag acggcucuac cagcgcacca agcugagacg ggaacgccuu      240

cauagaucuc ugaaugugcu cggauuccug ccugagcacu auaaaaacga aauugacuuu      300

gagcaacgga agggccaguu cuacccgaau aaggagccca aaaucgcuua uuacccugaa      360

caggucgcca gcaagguuaa auaucaguuu cuguucaagu ccagcuuucu cgagauggug      420

gaagauuuca aacgcagagg cuacaacggg gccauucccu acgacuggac aaucuauuac      480

cugcggaaga aggcucuuga gaaaaagauu gagaaagagg agcuggccug gcuccugcuu      540

aauuuuaacc aaaagcgcgg auauuaccag cugagaggcg aagaggaaga uaccaauucu      600

aacaaaaagg aggaguucua ugaacucaaa guguccagcg ucgagcagac acaugacacc      660

ucuccuuccg ggacguggua caaugugcau cugcagaacg gauggguuua ucggcgcaag      720

agcaaggauu cucugaaaga cugggaaggc cucaauaaga aauuuaucgu gaccacacac      780

cugaacgcug auggguccga gaaacuugac aaagauggaa augucagcag auccuuccgg      840

gcccccaagg aagacgacug gggccuggug aagaaaagaa ccgagucugu ucucgaaaag      900

agcggccugc acguggggga auuuauuuac gagucccuuc uggaaaaccc ugagcaaaag      960

auccgcggaa gccucaucaa gacaauugaa cggaaauacu auaagaagga gcuggaggcg     1020

auccuggaua aacagaccuc uuuccacaga gaacuccgca augacgcccu guacaaggag     1080

gcuacgcuug aacuguaucc gaaaaacucc gcccaucgga gcaacauuaa gcagagaggc     1140

uucaaauacc ucuuuaucga ggauauuauc uucuaucaac gcccccugaa gucucagaag     1200

caccuuaucg cucauugcca guacgagaaa cggacauaua agaauaacgg gggagaaacc     1260

auugagguca gccugcaggg cgccccuaaa ucccaccccu cuuuucaaga auuuagaauc     1320

uggcaguuua uuagcaaucu ccgcuucuac gacaaggagg aaaucgugga ggggcaggag     1380

guguccauua cagaaaaacu gcucaagagc gauucugacu uugcggagcu guaccacugg     1440

cugucccauc agcgggaagu cgaucaaaac aaaacccuua aguauuucac ccagaagaaa     1500

cugaucccua agcagaaaaa ggacaauccc acaaucagau ggaacuuucc ggaggauaaa     1560

aaguacgaaa ugggagaguu caaggcccag cuccugagcc gccuggagaa aguucggaau     1620

ugucaaccug acaaguuucu uacgcagaaa aacgaacucg aucuguggca cauuaucuau     1680

ucugugaccg acaagacaga guucgaaaaa gcucugguga ccuuugccga gaagagagaa     1740

cucgacuccg agagcuucgu cgagaauuuc aagaaauuuc ccccuuucaa gaacgauuac     1800

gcuuccuauu cucugaaagc cauuaagaaa auccuucccc ugaugcggcg cggcaaguac     1860

uggaaggccg aaaaucucgg cccccagaca cugcagagaa uugagaaaau cgugaacggg     1920

gaagcugacg auaagauuag cauccgggcg agaaaacugg cccuucaucu caaugacacc     1980

aaguccuuuc aaggacugcc uacgucucug gcuugcuaug uugccuacaa ccgccacagc     2040

gagaaugagg aaaaccagaa auggcagaca ccguccgaua ucgacaagua ccucaaagau     2100

uucaagcaac auagccugcg gaaccccauu guggagcagc uuaucaccga agcccugaga     2160

gucgugcgcg acauuuggaa gcacuauggc caggggcaga aagauuucuu cgacgagauc     2220

cacguugaac ucggaagaga gaugaagaau ccugcuauua aacggaagaa acugucucaa     2280

aagauccagg agaacgaaaa uaccaacuac cgcaucaagg aggugcugag aaaucuuaaa     2340

gaaaacaaua uugaugccaa gcccuauucc ccuagccauc aggagauccu caaaauuuac     2400

gaagagggcg ucuaucaguc uaucgagaac guggaagacg agauugaaaa gauccggaaa     2460

aauaacacac ccuccagcaa ggauaucaau cgcuacagac uguggcugga gcaaggguau     2520

auuucuccgu acaccggcga agucaucccu cucagccagc uguuuuccaa ccaguacgag     2580

auugagcaca ucauuccccg gagccgcuuc uuugacgacu cucuugcgaa uaaggugauc     2640

ugugaaucca gcguuaacgc ucugaaagau aauaagacag cccucgaguu caucacgaac     2700

cauggaaacg aaaaagugga guuugccggc aagaccgucg agauucuguc cucugacaaa     2760

uauacagaac acugcaagag auacuucaag cggaaucgca ccaaacugaa gaaccuucuc     2820

agcuaugaug ugccugaggg guuuaucaau agacagcuga acgacucccg guucauuucu     2880

aaauacguua gaaaucugcu cagcaacauc gugcgcgaag aggaggaacg ggaggcuauu     2940

uccaagcaca uccugacaau ucccggaucu aucaccagcc aacuuaaaca ggauuggggc     3000

cugaaugaaa aguggaacga ggucgugacg cauagauucg aacgccucaa uaaccugacc     3060

gggacaaccg acuuuggauu cugggauaau aagaucaacg ccuuucggau uacagugccu     3120

aaagagcugu ccaagggcuu caauaaaaag agaaucgacc accgccauca cacccuugau     3180

gcucugguua uugccgcgug uacgaaagac caugucaacu auaucacaag ccucaauacc     3240

gaacggaaca accacucucu gguguccaag cucagaaaua ccaaaaaggu gcugauuaac     3300

gggaaggagc gccggaucgc caaggcuuac aaacuucccu ggagcucuuu uccgcuggau     3360

gccaaacacc agcucgagac aaccgucauc uccuucaagc aaaaucugag agugauuaac     3420

aaaagcucua auaagacaug gcaguggcgg aacguuaaug gcgaacagaa gaaaaagcug     3480

aucccucaga cgaaaggaga cuccugggcu auucgcaaga gcaugcauaa agccaccgug     3540

uccgcgaagg ucgagagcgu gggcaagggg aaaaucaaga caaccaacag aacaccccuu     3600

ggagaaauua aaggcgagaa gcacguugag aaaaucuaug augcccggau ccgcgaagug     3660

auuaucccca aucaucugaa caaguaccuc gacgagaagg ggaaaccuaa uuacgaagcu     3720

gccuuuucuc aaagcggaau uaagcagcug aacgagaaua ucgaaguccu caacgagggc     3780

aaaccgcacg cucccauuua uaaggugccu cuguucgagg uuggguccaa auuucccgug     3840

ucugaagacg ccaagagccc uaaaaaucag aaguacgucg aggccgauaa gggcaccaac     3900

cuuuucuuug cuguguauug gaaugaagag aaacagaaga gaaacuucga aacggugccc     3960

cugcaucaag ucaucguuca ccagaaacag guggcguccc ucccuacaga gcagcggacc     4020

ucugugccca uuaagccgga gcugggacug uuccuuuuua gccugucccc uaacgaccuc     4080

gucuacgugc ccaccgaaga ggaaaucgag aauccucacc ugguugaucu caaaaaccug     4140

ucuaaggacc aaacaaauag aaucuauaag auggugagcu ccaccggcaa cgaaugccau     4200

uucauucccc accauaucag ccgccuuauu augaaauaca augccaagca gaaagucggg     4260

gaguuuggau cucugaacaa ggcugagaaa gccaucacag gcgaaacgau uaaggagaag     4320

ugucugaaaa uccagaucaa uucccucggg aagcugaaaa ccgaacggau ugccuga        4377


<210>  4546
<211>  4380
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4546
augaaguuca accugggccu cgacaucggg accaauagca uuggaugggc ccuggugaac       60

gaggauuacg aaaauaaaaa gggcaaaauc auugcugccg gguccagaau cauucccaug      120

acacaggaca uccuugauaa guuuggaucu ggcgcgauca cggagaccca aacagcucug      180

cggaccgacu aucgcagcaa aagaaagcuc auucagcggu uccugcuucg cagagaacgg      240

cugcaccgcg uccucaacgu ucuggauuuu cugccuaagc auuacgccga guccaucgac      300

uucgaaaaau auuuugggaa guuccucccg aauacagagc ccaaacuggc cuacaaccag      360

aaucaguuua uuuuccaaaa cucuuuucuu gagaugcuga aggaauuuca ggagaaacac      420

ccugaacucc ugaccaauaa caagaagauc cccuaugauu ggacaauuua cuaucuuaga      480

aagaaggcuc ugaaucagcg gaucgagaaa gaggaacucg ccuggcugcu gcuccaguuu      540

aaccaaaagc gcggauacaa ucagcugaga gacgaggaag aggaaagcgu gccuaacaaa      600

cagguggagu ucuacucccu uaagauuauc gaugucgugg cugaccagcc caaagagggc      660

cgggauaagc gcugguauuc uaucaaucug gaaaacggcu ggaccuacag acgggagagc      720

aagacgccuc ucuuuaacug ggaagggaaa accaaggacu ucauuguuac aaccgaucug      780

aaucaagacg gauccaucaa aaaggacaaa gagggcaagg aaaagcgcag cuucagagcc      840

ccgucugagg gggauuggac acuucugaaa aagaaaaccg agaaggaaau uuaugagucc      900

ggaaaaacgc ucggcacaua caucuaugac gcgcugcuga acacccccga ucucaagauu      960

aagggggacu uuauccggac aaucgauaga agcauuuaca aagaggaacu gcagcagauc     1020

auuaauaagc aaaaagaaua ucaucuugag cugaacucuu ccaagcucua caaagccugc     1080

cuggaggaac uuuacccuaa gaaugagauc uauaagagcg cucugaaaaa caaggacauu     1140

gccuaccucu ucaucaauga uauccuguau uaccagcgcc cccugaaagc uccuaaguau     1200

aacauuggaa aauguaccua cgaaucucgg accuuuaaga aagaggacaa gaagaucaga     1260

gaguauauuc ccgccguggc gaaaucccac ccccucuucc aggaauuucg ccuguggcag     1320

uggaucaaga aucuuaaaau uuacgagaag cugagcuccg auaacugcac agacuucuuu     1380

cucacgaaug aacaagauuu cgagaaccug uuugaguucc uguggaauaa aaaggaaauc     1440

gaccauaagg gcauucuuga guaccucguc aaaaccaagu uuccugaccu gaaacccaaa     1500

caggugaaaa caaagguuaa ggaauaucgg uggaacuacg uguaugauca cgagaaagac     1560

gaaucuaaga ccuacccuug uggcgagaca uauuuccugc ucaaaaauaa gcuggauaaa     1620

aucuacaacg cccaggacga uuuucuugac aaggaaagac uguauaaccu cuggcacauc     1680

auuuacagcg ucaccgauaa gaaacaguac gagaaggcuc uggccaaauu cgcucugaag     1740

aaucaucuug acacgaaauc cuucgugaac acauuuaagc gcaucccgcc cuucaaaaau     1800

gauuaugcca uuuacagccu caaggccauc aagaaacugc ugccucucau gcgggugggg     1860

aaguauugga acuacgacga uauugacccg aucaccaaac aaaagaucca gugcauuauc     1920

accggagagg aaaaugauac aauuucuacc cuggucagag agaaaacaaa ccacuuuucc     1980

aaugaaaacg acuucaagaa ucuuccccug uggcucgcug gcuauaucgu uuacaacagc     2040

cauaaugagu cuagcgaaau ugagaagugg aaauccccuc aggacaucga uacguuucug     2100

aagaaauuca agcagcacgc gcugcgcaac cccaucgugg agcaaguggu caccgaaaca     2160

cuucgggugg uuagagacau uuggucuaaa aaggggcagg gaaagaaaga uuucuucaau     2220

gagauccaca uugaacucgg caaggaccug aaaaacccug uggauaagcg ccggaaacug     2280

agcuccacca auacaaagaa cgagagcacc aacacgagaa ucaaggcccu ccugauugag     2340

cuuuuuaccc agggggucga aaaugugaaa cccaacucuc cgaagcauca ggagauccug     2400

aaaaucuucg aagagggagc ucucaauucc ggcauugaaa ucgaggagga cauuaucaag     2460

auuagcaaca cagaucaacc uaccaaaaag cagcuggaac uguauauccu uuggcucgag     2520

cacaaguaca aaucucccua cacaggccag uccauuccuc ugagcaagcu guuuaccccc     2580

aauuaugaaa ucgagcauau cauuccucgg uccaaauacu augacgauaa ccucucuaau     2640

aagguuaucu gugaaacgga gauuaaccgc cugaaaaaua accaacuugg guacgaguuc     2700

aucaauaacc acgaaauuag agaggugaca cuggaagagg gaaagaccau ccggcuccug     2760

agccccgaac aguauuggaa uuuugucaca accuacuaug ccaaaaacaa ggccaagaga     2820

gagaaucugc uucugaaaga gcucccggug cagauggucg aaaagcagcu gaacgacacc     2880

cgcuacgugu ccaaauacgu uaugcaacuc cugucuaaga ucgugcggaa agaggauggc     2940

acagacaacg ggauuaauag cacgaacauc cuuuccauua gcggaaagau gaccagagcu     3000

cugaagaaug auugggaccu caacgacguc uggaaugauc ugcugcuuua ucgcuucgaa     3060

agacugaaca caaucaccaa aacaaaucuc uucaccacgu acucugagca gaagcagaaa     3120

cggaugccua uugugcccga ccaguaugcc aagggcuuuc auaaaaagcg ccuggaucac     3180

agacaccaug cgcucgacgc uaucguuauc gccugcauua cacggaacca caucaauuac     3240

augaacaauc aaaacauuca gggggagaag cugaaaaaga augccaucga aaaagagaac     3300

uaucgcgaaa uucuucagaa guccagacug gauauccggg cucagcucug ugagaaaaag     3360

uacaaugaca acgaucccac caaguauaau uggguguuca uuaaaccuug ggccgacuuu     3420

accgaaaaca caaaggauau ccuggagagc aucauuccgu cuuucaaaca aaaccugcgc     3480

aucauuaaua agaccgucaa cuacuacgag aaauauaaug aaaacggcaa gaaagugaag     3540

gugagacaga ucaagggaga caauugggcu auucggaaac cccuucauaa ggagacaauc     3600

uccggcaaag uucaccugag cuacaagacg ccugccaaag ggaagauccu caccgcgaca     3660

cgcaaggucc uggauucuag cauggacuuu aaauccauug aaaagaucac cgacacaggc     3720

auucagaaaa uccucaagaa cuaucuggag uacaaaaaga gccccgaacu ugccuucucu     3780

ccugagggaa uugaggaacu gaaugcuacc aucacgaagu auaacaaugg guuucaccau     3840

aaacccaucu acaaggugag aauuuaugag gaaggaguga aauucccucu gggcaacaag     3900

gggaauaaaa aguccaagua cgucgaggcc gaaaaaggca ccaaccucuu uuucgcuauc     3960

uacaaggauc aagagaauaa ccgguauuuu gagacaauuc ccuucaauga cgugcuggaa     4020

acccuuaaaa agggaaugag cgccguuccg gagaaaaacc aggaagagaa cacacuggug     4080

uucgcgcucu ccccucagga ucuggucuuu gugcccaccc ucgacgagau cgaaaauccu     4140

gauauuguug acuucaagaa ccugacaaaa gaucagacca auagaaucua caagauggug     4200

ucuagcuccg gcaagcaaug cuuuuucauu aaagccgagg ucgcuacguc uaucgugaac     4260

aagauggaau uuagcacauc caauaaaaug gagaacucua ucgaagggau ucagaucaag     4320

agcaauuguu ggaaacuuaa gcuggaccgc cugggaacca uuacccgggu uaucaaguga     4380


<210>  4547
<211>  4383
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4547
augaccaagu ucaaccuggg ccucgacauc gggacaaaua gcauuggaug ggcccuggug       60

aacgaggauu gcgaaaauaa aaagggcaaa auccuugcug ccggguccag aauuaucccc      120

augacgcagg acauucugga uaaguuugga ucuggcgcgg cuaccgagac acaaaccgcc      180

cuccggacag acuaccgcag caaaagaaag cugauccagc gguuccuucu gcgcagagaa      240

cggcuccacc gcguccugaa cguucuggau uuucucccua agcacuaugc cgaguccauc      300

gacuucgaaa aauaccuggg gaaguuucuu ccgaauaccg agcccaaacu gguguauaac      360

cagaaucagu ucauuuuuca aaacucuuuc uuugagaugc ucaaggaguu ccaggaaaaa      420

cacccugaac uguuucuuaa uaaccagaag auccccuacg auuggacaau uuauuaccug      480

agaaagaaag cucucagcca gaagaucgag aaagaggaac uggccuggcu gcuccugcaa      540

uucaaucaga agcggggaua uaaccagcuu cgcgacgagg aagaggaaaa uaccccugau      600

aaacuggugg aguuuuacuc ccucaagauu aucaaaguca cggcugacga ggccaaggaa      660

ggcauuaaca agagauggua cucuaucaau cuggagaacg gcuggaucua ucggcgcgaa      720

agcaaaacac cccuuuucaa cugggagggg aagaccaaga auuucauugu gaccacagau      780

cugaaccagg acggauccau caagaaagau aaggaaggca aggagaaacg gagcuuuaga      840

gcgccuucug agggggacug gacccuccug aagaaaaaga cggaaaaaga gauuuacgaa      900

uccggaaaga cacugggcac cuauaucuac gacgcccucc ugaauaaccc ggaucuuaag      960

auuaaagggg cucugaucaa gacaaucgac cgcagcauuu auaaagagga acuccaacag     1020

aucauuaaua agcagaaaga guaccauucu gagcugaaca auuccaagcu guauaaggcc     1080

ugucuugagg aacucuaccc caaaaacgaa aucuacaaga augagcugaa aaacaaggau     1140

auugcuuauc uguucaucaa ugacauccuc uacuaucaaa gaccucugaa agcccccaag     1200

uacaacauug gaaaaugcag cuaugagucu cggaccuuua agaaggaaga uaaaaagauc     1260

accgaguuca uuccugcggu ugccaaaucc cacccccuuu uucaggaguu cagacugugg     1320

caguggauca agaaucucaa aauuuacgaa aagcugacaa acgacaauua uacggacuuu     1380

uuccugacca acgaacgcga uaucgagaaa cuuuuugauu uccucuggaa caaaaaggag     1440

auugaccaua aaggcauccu ggaauaccug aucaagacaa aauuuccgga ucucaagccu     1500

aaacagguga aaagcaaggu caaagaguac cgguggaauu auauuuacga ccacgaaaag     1560

gacgagucca aaaacuaucc cuguggcgaa accuacuucc ugcuuaagaa uaagcuggau     1620

aaaaucacau cuauucaaga cgauuuucuc aacaagaaaa gacuggagaa ucuguggcac     1680

aucauuuaua gcgugaccga caagaaacag uacgagaagg cucuugccaa auucgcucuc     1740

aagaaccagc uggauacgaa guccuucguu aauacauuua aacgcauccc ugccuucaag     1800

aacgacuaug ccaucuacag ccugaaagcu auuaagaaac uccugccccu uaugcgggug     1860

gggaaguacu ggaauuauga ugacaucgau cccauuacca agcagaaaau ccaaugcauu     1920

aucaccggag aagagaacga cacaaucucu acccugguca gagaaaagac aaaucauuuu     1980

uccaacgaga augauuucaa aaaccucagc cuguggcugg cgggcuacau uguguauaau     2040

ucucacaacg aaagcuccga gaucgagaag uggaaaucuc cucaggacau ugauacguuu     2100

cuuaagaagu ucaaacagca ugcccuccgc aacccgaucg uggaacaggu cguuaccgag     2160

acacugcggg uggugagaga cauuuggcug aagaaagggc aaggaaagaa agacuuuuuc     2220

aaugaaaucc acaucgagcu cggcaaggau cugaagaacc ccgucgacaa acgccggaag     2280

auuagcucca ccaauagcaa aaacgagaca accaauauca gaauuaaggc ccuucugaaa     2340

gaacuccuga aggaggggau cgaaaacauu aagccuaauu cucccaaaca gcaggagauc     2400

cugaagauuu uugaagaggg aauccuuaac uccggcaucg auauugagga agagaucauu     2460

aaaaucagcc agauugcuca accuacgaag aaacagcucc aguacuaucg caauuggcug     2520

gaacaaaagu acaucucucc cuauaccggc caguucaucc cgcuguccaa acucuuuaca     2580

agcaaguacg agauugaaca caucauuccu cgguccaagu acuaugacga uucucugagc     2640

aacaaaguga ucugugagac cgagauuaau agacuuaaga acaaucagcu gggguacgag     2700

uucaucaaca aucaugaaau ccgggaaauu acacucgagg aaggaaaaac cauccgccug     2760

cuguccccca agcaguaucu ugacuuuguu acgacauacu auggcaaaaa caaggccaag     2820

agagagaacc ugcuccugaa agagauuccu auccaaauga uugaaaauca gcucaacgau     2880

acccgguacg ugucuaagua ugucaugcag cugcuuagca aaaucgugag aaaggaggac     2940

gggacagaua auggaaccaa cuccauuaau auccugagcg uuucuggcaa aaucacccgc     3000

gugcucaaga cagacugggg gcugaacgau gucuggaaug accugcuuug cuaccgguuc     3060

gaaagacuga acaagauuac gaauaccaac cucuucacaa ccuacuccga gcagaaacaa     3120

aagaucauuc ccaucgugcc ugaucaguau gcuaaaggcu uucacaagaa aaagcuggac     3180

caucgccacc augcccucga cgcgauucug aucgcuugua ucacaagaaa ucacauuaac     3240

uacaugaaua accagaauau ccagggagag aaacuuaaga acuucaagau ugauaaacac     3300

aauuaucaug aaaucuuuca aaagagccgg cuggacaugc gcgcccagcu cugcgagaaa     3360

aaguacaacg aucacgacuc uaccaaauau acgugggucu ucacaaagcc cugggccgau     3420

uuuaccgaaa acauuaagga cacccuggag uucaucaucc cguccuuuaa acagaaucug     3480

agaauuauca acaagacagu gaauuacuau gaaaaauaca acgagaaugg caagaaaguu     3540

aaggugcggc aaaccaaggg ggauaacugg gcuauucgca aaccucuuca uaaggagaca     3600

aucagcggaa aaguccaucu gcucuacaag acgcccgcca aaggcaagau ucugaccgcu     3660

acaagaaagg ugcucgacuc uagcauggau uucaaaucca ucgaaaagau uaccgacaca     3720

gggauccaga aaauccugaa gaauuaucuu aaauacaagg agagccccga acuggccuuu     3780

ucuccugagg gaauugagga acugaacauc accauugcga aguauaauga uggcuucuac     3840

cacaaaccga ucuauaaggu ucggauuuac gaggaagggg ugaaauuucc ccucggcaac     3900

cgcggaaaua agaaaaacaa guaugucgag gccgaaaaag gcacgaaucu guucuucgcu     3960

aucuacaagg accaggagaa caauagauac uuugaaacca ucccuuucaa cgacgugcuu     4020

gagacacuga agaaagggau guccgccgug cccgagaaga accaggaaaa gaauacccuc     4080

guuuuugcuc ugagcccuca agaucucguc uucgugccca cacuggacga gauugaaaac     4140

ccugauaucg uggacuuucg gaaucuuacc aaggaucaga caaaccgcau uuauaaaaug     4200

gucuccucua gcggaaagca guguuucuuu aucaaggccg agguggcgac cuccauuguu     4260

aauaaaaugg aguucacgac aucuaacaag auggaaaaua gcaucgaggg cauccagauu     4320

aaauccaacu gcuggaagcu gaaacuggac agacucggga ccaucacccg ggugauuaag     4380

uga                                                                   4383


<210>  4548
<211>  4389
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4548
augaccaaga acauccuggg ccucgaccug gggacaaaua gcauuggaug ggcccuuauc       60

aaacagaacu ucgagaauaa gcaaggcgaa auucugggga ugggauccag aaucauuccc      120

augucucagg auauccucgg cgacuuuggg aaaggaaaca gcguguccca gacggcugag      180

cggaccaagu accgcucugu cagacggcug cgcgaaagau uccuucugcg gcgcgagaga      240

cuccaccggg uucugaaucu gcucaacuuu cugccucagc auuaugccag ccaaaucgau      300

uucgaaaaac gcuuuggcaa guucaaggug gagacagagc cgaaacuugc guggaagaau      360

auugacggcc aguuuuccuu ccuguuucag accucuuuca acgaaaugcu cgaggauuuu      420

agagugaaug ggcaggacgu caaaaucccc uacgauugga caauuuauua ccugcggaag      480

aaagcucuua gcaagaagau cgaaaaagag gagcuggccu ggauucuccu gaacuucaau      540

caaaagcgcg gauauuacca gcugagaggc gaagaggaag aggaaaaccc uaauaaacuc      600

guggaguuuu auucccugaa gguuguggac gucguggccg augagcccca gaaagggaag      660

uucgacaucu gguacagccu uauucuggaa aacggauggg uuuaccggag aucuuccaaa      720

aucccucucu ucgauuggaa ggacaagacc cgcgauuuua ucgugacaac cgaccugaau      780

gacgauggca gcauuaaaaa ggacaaagau gggaacgaga agcggucuuu cagagcuccc      840

uccgaaaacg acuggacgcu ugucaaaaag aagaccgagc aggaaaucga gcaaagccgc      900

aaaacagugg gaaccuauau uuacgaggcc cugcucgaua auccuaagca gaaaaucaag      960

ggcaaacugg ugcggacaau ugaaagaaag uuuuauaagg aggaacugcg ccagauccuc     1020

gagaaacaaa aggaauuuca ccaggagcug cagucugacg aucuuuacaa cgacugcguc     1080

cgggagcugu auagaaauaa cgaagcucau cagcucaccc uguccaaaaa ggauuuuguu     1140

caccuucuga uggacgaucu caucuucuac caacgcccgc ugcggagcca gaaauccucu     1200

gugagcaauu guacgcugga guuuagaaag uauaaggacg aaaacggcgc cgagcauaca     1260

caguaccuca aagcgauucc caaguccaau ccuuacuauc aggaguuccg ccuguggcaa     1320

uggaucuaca accuuaauau uuaucggaga gaagacgagc agaaccccau caccggggaa     1380

uuucugagca caaccaaaga guucgaaaau cucuuugagu uccugaacac ccgcaaggag     1440

guggaucaga aagcccugcu uaagcacuuu aaacucaaug acaagacaca ccgguggaac     1500

uucgucgaag auaagaaaua cccuugcaau gagacgaaga ccaugauuuc uuccagacug     1560

gaaaaagugg agaacaucag cgacgauuuc cugacacggg aaaucgagca gaagauuugg     1620

cauaucauuu auucuguuaa cgacaaaauc gaguacgaaa aggcucucaa gagcuuugcc     1680

aaaaagcacc aucuggauga guccucuuuc uuugaggcuu uccgcaaauu ucccccguuc     1740

aagagcgaau auggauccuu uagcgaaaaa gccgugaaga aacuucugcc ucucaugaga     1800

cugggcaaau acuggaauua cgcggacauu gauaagucuu ccaaagagcg gauccaaaag     1860

auuaucagcg gggaguauga cgaaaacauc aaagauagag uccgcgagaa gucugugcac     1920

cugaccacag aaaaugacuu ccagggacuu cagcucuggc uggcccagua cauuguuuau     1980

ggccggcacu ccgaggcuag caugaucggg aaguggaacu ccgccgauga ccuggaggaa     2040

uuucucaaaa auuucaagca acauucucug agaaacccca uuguggagca ggucaucacc     2100

gaaacgcuuc gcguggucaa agacauuugg cugaaguacg gaaauggcgc uaaagauuuc     2160

uucaacgaga uccacaucga acucggcaga gagaugaagc ugccugccaa agagcgggaa     2220

gaccugacac gcaagauuac cgagaaugaa aacaccaauc uuagaaucaa ggcccuccug     2280

gcugagauga ugaacgauag cuccguggaa aauguucggc ccuuuucucc caugcagcaa     2340

gagauucuga aaaucuauga ggacggggug cucaacagcg gaauugaaau cgaggaugaa     2400

uaccugaaga ucuccaaaac agcgcagccu agcccgucug accuuaagcg cuauaaacug     2460

uggcucgagc agaaguacaa gucccccuac accggccaga uuaucccucu gaauaaacug     2520

uucacacccg aauaugagau ugagcauauc auuccucaaa gcagauacuu cgaugacucu     2580

uuuuccaaca agaucaucug ugaaagcgcc gucaacaaac uuaaggauaa uuauauuggg     2640

cucggauuca ucaaacaguu uggcgggacg auuaucgagc ugggcuucgg aaagucugug     2700

aagguuuuug aaaccgagga guacgaagac uucgugaaaa agcacuaugc uaacaaucgg     2760

agcaaacgca acaagcugcu ccuggaggau auucccgaaa agaugaucga gagacagauu     2820

aaugacacac ggcauaucuc caaguacauc agcggcgucc uuucuaacau ugugcgcauc     2880

gaugacgacc aggccaagaa aauuaucgaa gaugccgaga uucuggcuag aaaggagauc     2940

gacaaagaua aggccaauga aauccggaga aaagcgaagu cccucgcuac cgacgauggg     3000

auuaacagca agaauaucgu gccgggaaac ggcaaaauua caacccaacu gaagcaggac     3060

ugggggcuga augauguuug gaacgaccuu auccugccuc gcuuugagcg gaugaaucag     3120

cucacgaacu ccaaagauuu caccgucugg aaugaaaacc accagaagua ucugcccaca     3180

gugccuauug aguuuucuaa aggauucagc aagaaaagaa ucgaccaccg ccaucacgcc     3240

cucgaugccc uggugaucgc uugcgccacc aaggaccaug ucaaccuucu gaauaaccaa     3300

uccgcuaagu cugacacaaa acgguacgau cucaagaaaa agcugaugaa auuugaaaag     3360

gugguuuacc accacaccca gacgggcaau aagauugaga gagagauccc caaacaguuc     3420

cugaagccuu gggaaaacuu cacauuugac gccaaauaua aucuugagag cauuaucgug     3480

uccuucaagc aaaaccugcg cgucauuaau aaagcgacca acuacuauga aaaguacgug     3540

gagaaggaug ggaccaaaaa uaagggccgg guugaacaga caggaaccaa cugggccauc     3600

agaaaaccca ugcauaagga cacagugucu ggcaaaguca aucucccgug ggugaagguu     3660

ccuaagggga aaauucugac ggcuacccgc aagauccucg auaccagcuu ugaccugaaa     3720

uccaucggaa gcauuacaga uacaggcauc cagaagauuc uucggaacua ucuggaguuc     3780

aaagggucuc ccgagcucgc cuuuuccccc gaaggaaucg aggaccugaa uaagaacauu     3840

gaaaaauaca augauggcaa gccucaccag ccgaucaaca aggugagagu cuucgagcug     3900

gggagcaaau uucaaguggg ccagaccgga aauaagaaag acaaguaugu ggaggcugcc     3960

aaaggcacga accuuuucuu ugcggucuac gaagauaaga acgggaagcg gucuuacgag     4020

acaauccccc ugaaugaagu uauugagcgc cagaaacagg gacucuccgu gguggaccug     4080

aagggcacca acgauuucua ucucuguccu aaugaccugg ucuacauccc cagcgacgau     4140

gaacuugaga acauuaauaa caucgacuuu aaaaauauug ccaaggagaa aaacgaaaga     4200

aucuauaagg ugguuucuuu cuccgggagc caaaucuuuu ucgugcggca ggauauugcu     4260

accuccaucg ucaauaaggc cgagcugagc acacugaaca aaauggaacg cgcuauggac     4320

ggaucuauga uuaaggagag cugcgugaaa cucaauguug auagacuggg caacaucucc     4380

aaggccuga                                                             4389


<210>  4549
<211>  4401
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4549
augaccaaga acauccuggg ccucgaccug gggacaaaua gcauuggaug ggcccuuauc       60

aaacaggauu ucgagaacaa gcaaggcgaa auucugggga ugggauccag aaucauuccc      120

augucucagg acgugcucgg cgaguuuggg aaaggaaaua gcgucuccca gacggcugcc      180

cggaccgauu accgcggcgu uagacggcug cgcaagagau uccuucugcg gcgcgaaaga      240

cuccaccggg ugcugaacgu gcugaauuuu cucccugucc auuaugcguc ucagaucgac      300

uucgagaaac gcuuuggcaa guucaaggaa gauacagagc cgaaacuggc uuacaacaag      360

gagggguuua ucuucaaaaa uagcuuugaa gagauggugg ccgacuucag aaagcaccaa      420

ccccagcuuc uggaaaacga uaaaaagauu ccuuaugacu ggaccaucua cuaucuccgg      480

aagaaagccc uguccaagaa aauugagaag gaggaacuug cuuggauccu gcucaauuuu      540

aaccagaaac gcggauacua ucagcugaga ggcgaggaag aggaagagaa ucccaacaag      600

cugguugagu ucuacucucu caaaauuaca gaugugcugg ccgacgaacc ucaaaaggga      660

aagagcgaua ucugguacuc ccuuauucug gagaaugggu ggaucuaucg gcgcagcucu      720

aaaacacccc ucuuugacug gaaggaaaag accagagauu ucaucgucac cacggaccug      780

aacgacgaug gcuccauuaa gaccgacaaa gaugggaaug agaagcggag cuucagagcu      840

ccuucugaaa acgacuggac acuugugaag aaaaagaccg agcaggagau cgauaaaucc      900

cagaagacag uuggaaccua cauuuaugaa acgcugcucc agaacccgaa acaaaagauc      960

aagggcaaac uggugugcac aauugagcgc aaguuuuaca aagaggaacu gaagcagauc     1020

cucgaaaaac agaaggaguu ccaucaagag cugcagaaug acgaucuuua uagcgacugu     1080

guccacgaac uguaccggac caacgaggcc caucagcuca cacugucuaa gaaagaaauc     1140

gugaaucuuu uuauggagga ucugauuuuc uaucagagac cccuccgcuc ccaaaagacc     1200

agcaucucca acugcacccu ggaguuucgg aaauacaagg acgaaaaugg cguggagcac     1260

acacaguucc ugaaagcgau uccuaagucu aaccccuacu aucaggaauu uagaaucugg     1320

caguggauuu ucaaucucaa caucuacaaa cgcgaugacg acagcaaugu cacgaaggag     1380

uuucugaaca ccacagaaga uuucgagaau cuuuuugagu uccugaacaa ucggaaggaa     1440

guugagcaaa aagcccuccu gaagcacuuu aaacugaacg aaaaacuuca uagauggaau     1500

uucguggagg acaaaaagua uccuuguaac gaaaccaaga caaugaucuc cacccgccuc     1560

gauaaagugg agaacauuag caaggacuuc uuuacgcggg agaucgaaca gaaaauuugg     1620

cacaucauuu acucugucaa ugauaagauc gaguaugaaa aagcucugaa guccuucgcc     1680

auuaagcaua accuggacga gaauagcuuu uucgaggcuu uuaaaaaguu ucccccguuc     1740

aaaucugaau acgggagcuu cuccgagaag gccaucaaaa agcuccugcc ucuuaugaga     1800

cugggaaagu auuggaacga agaugagauc cucaaauacu cugacgauua cuuuaagaau     1860

auugaaagcc ugcugggcgu gcuuaaacag cgcgacgaga acaucuccga ugcggaccgg     1920

gagaauuaua acaagacaau uaauaacaaa cucagagccg aacuggagaa uuuccagaac     1980

gcugaaaucg agagcuuuca agggcugcgg cuccacauug cccaguacau ccuguaugga     2040

aagcacucug aaggcgauaa uaaaaaguuc aacuccgcug acgaccuuga gaaguuucug     2100

aaagaguuca agcagcauag ccuccgcaau cccaucguug aacaggugau uaccgagacc     2160

cugagagucg ugaaagauau cuggacaaag uacgggaacg gagccaaaga cuucuuugau     2220

gaaauucaca ucgagcuggg ccgggacaug aagaagacca gagaugaacg cgccgacgcu     2280

acaaaaauua ucacggagaa cgagaauacc aaccuucgga ucaaggcgcu ccuggccgaa     2340

augaugaaug auguugagau ggaaaacgug agaccuuauu cucccaugca acaggagauu     2400

cugaaaaucu acgaagacgg cauucucaag uccgauaucg agauugagga cgauauccug     2460

aaaaucagca agacagcuca gcccuccucu agcgaccuua agcgcuauaa acuguggcuc     2520

gaacaaaagu acaaaucccc uuacaccggg cagauuaucc cgcugaauaa gcuguucaca     2580

cccgaguaug aaauugagca uaucauuccu cagucuagau acuuugauga cagcuucucc     2640

aacaaaauca uuugcgagag cgccgucaau aaacuuaagg acaacuauau cggacucggc     2700

uuuaucaaac aguucggggg aaccauugug gaauguggcu uugggaagaa ugucaaagug     2760

uucgaggccg aagaguacga agauuuuguu aagaaacacu augcuaacaa ucggggcaag     2820

cgcaacaagc ugcugcucga ggagaucccc gaaaagauga uugagagaca aaugaaugac     2880

acgcggcaua ucucuaagua cauuuccgga gugcugagca acaucguccg cguggaagau     2940

ggcucugacg aggggguuaa uuccaaaaac aucgugccug gaaacggcaa gauuaccaca     3000

cagcuuaaac aggauugggg gcugaaugac gucuggaacg aacucauccu gcccagauuc     3060

gagcggauga aucagcugac caacagcaca gauuuuaccg ccuggaauga gaaccaccaa     3120

aaguuccuuc cgacggugcc uauugaguuc ucuaaaggau uuagcaagaa gcgcaucgac     3180

cacagacauc acgcgcugga ugcucucauu aucgccugcg ccacaaaaga ccaugugaau     3240

cugcucaaca aucaguccgc uaagagcgau accaaacggu augaccugaa gaaaaagcug     3300

augaaguucg aaaaaguugu cuacaaccac ccccagacag gcgaccgcau cgaaagagag     3360

gugccuaagc aguuucuuaa acccugggau aauuucaccu uugaagccaa gaacaaucuc     3420

gacaaaauua ucgugucuuu caagcaaaac cugcggguca uuaauaaggc uaccaacuac     3480

uaugagaaau uuguggagaa ggaugggguu aaaacaaagg aaagagugga gcagaaaggc     3540

acgaauuggg ccauccgcaa gucccugcac gaagagaccg ucagcggaaa gauugaccuu     3600

ccuugggugg agacaaacaa cggcgaguuc aucaccgcga cacggaaauc ccuggaugcc     3660

ucuuuuaauc ucgaaaagau uaaaaagauc accgacacgg ggauccagaa aauucugaga     3720

aacuaccuca aggcuaaaga ugacaaugcc gaacuggcuu ucagccccga gggaaucgaa     3780

gaucuuaaca agaauauuga gaaguauaac gacggcaaau cccaucaacc gaucguucgc     3840

gugcgggucu acgagaaggg gaaaggaaga uuugcccugg gccagacagg gaauaacauu     3900

gauaaguaug ugcagggcuc uccuaaucuc uucuuugcga ucuacaaaga cgaaaacgga     3960

aagaaaagcu augaguccau ccgccuggau auuguuaucg aacggaugaa acagggccug     4020

caagccgugc ccgagaccaa ucccaacggg auuucucuuu accagaaucu gagcccucuc     4080

gaccuggucu acaucccgac cgaagacgag auugagagcc cccaccucau cgauuucaca     4140

auccugaaca agaccagacu ugaacgcauu uauaauguga acgacuucuc cggauacaca     4200

aucuauuuuu cucagaacuc cuucgcuaaa aauauuuacc cuaaggagau ggauaugacg     4260

uggaacgaaa agaagcagaa gcccagcggc ucuuuugacu ccaaaaccgc cagcuauaau     4320

aagaaaucua ucaaggauau uuucaucaaa cugaagauug accggcuggg gaacaucucc     4380

aaggugagca auacacucug a                                               4401


<210>  4550
<211>  4401
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4550
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugggc ccuugugaaa       60

gaggauuucg aaaacaagca gggcgagauc cuggggcucg gauccagaau uauccccaug      120

ucucaagaca uucugggcga auuugggcgg ggaaauagca ucucccagac agcugaucgc      180

acgggcuaca gaggcguccg gcgccuuaga gagcggcauc ugcuccgcag agaacggcug      240

caccgcguuc ugaacauccu cggguuccug ccuaaucacu augccaaaga gauugacuuu      300

accaagagac uuggaaaauu caucaaccau gcggagccga agcugucuuu ugauaaggag      360

uucauuuuua aagacagcuu caaugaaaug cucaaggauu uucagcagaa ccaacccggc      420

uucaucauua auaaacacgg ggaagaggcu cuggugccuu acgacuggac aaucuauuac      480

cuucggaaga aagcccugac ccagaagauu gauaaggagg aacucgccug gauccugcug      540

aacuuuaauc agaaacgcgg auauuaccag cucagaggcg aggaagagga agagaacaca      600

aauaagcugg uggaguucua uucccuuaaa aucauugacg ucaucgcuga uaacgaagug      660

aauaagaaag gggagguuug guacucucug caucucgaaa acggauggau uuaccggcgc      720

agcuccaaga ucagccuggu ggacuggaaa gauaagguca gagacuuuau ugugaccaca      780

gacguuaacg augacggcuc ugagaaacuu gauaaagacg gcguggucaa gcgguccuuc      840

agagccccca gcgcugauga cuggacccug cucaaaaaga aaacggaaca agauaucgac      900

aauucuaaca agaccguggg ggccuauauc uacgagaauc ugcugcucaa cccuaagcag      960

aaaauuaagg gaaaacuggu gcgcacaauc gagcggaagu ucuauaaaga ugaacuuaag     1020

cagauucugg agaagcagaa agaauuucac caagagcucc agaaugaaca gcugcuucaa     1080

gacugcguca gagagcugua caaguauaac gagcagcacc agcagaugcu cgaauccaaa     1140

gauuucguuc aucuguuucu gaaugacauc auuuucuacc aacgcccccu ccggagccag     1200

aagucucuga ucuccaacug uacccuugag aaaagaguga gccgcgacgg cguggucuuu     1260

ccuaucaagg ugauuuccaa gucuaauccg uauuaccagg aguuccggcu gcuccagugg     1320

cugcaaaacc uggcgaucua cagaaaagau gacgauguua augugacaca ggaauuucuu     1380

aacagcauug aguccuggga aaaucuccug caguuccuga acgccaagaa agagaucaag     1440

caggaacaac ucauuaaauu ucugcuugag cagaaggggc ugaaaggaaa uagccucaag     1500

accgcuaucc ugacguaucg cuggaacuac gucacagaca agguguaucc caugaacgaa     1560

acccggucuc ugauucagga uaaacuuaag aaaguugaga auguggccac agacuuccuc     1620

uccuuugaua acgagauggc ucuguggcac aucaucuaca gcgucaauga caagauugaa     1680

uaugagaaag cccuggcgaa guucgccucu aagcauaacc ucgaugaaau cagcuuugug     1740

gaguccuuca aaaaguuccc ucccuuuaaa ucugacuacg gcagcuucuc cgaaaaggcu     1800

auuaaaaagc ugcuuccucu gaugagauuu gggagcgucu ggaauaagaa caauaucgug     1860

cccucuguuc ucgaucgcau ugacaaaauc cugaccggag aguaugauga gaagaucaaa     1920

auucggguga gagaaaaggc cgagcaguuc caacugaacc agaucgaaga cuuucagaau     1980

cuuccgcucu ggcuggcuca auacgucgug uacgaccggc acuccgaggc cgaaauugcc     2040

ggcaaaugga acagcguuaa ggaucuggag aauuaucuca aggaguucaa acagcacucu     2100

cugcgcaacc cuaucgugga acagauuauc accgagacac uuagagucgu gaaggacauc     2160

uggcagcaau acggcaaugg ggugcagaac uuuuucgaug aaauucauau cgagcuggga     2220

cgggagauga aaaauacggc ugacgaaaga aagcgcauua ccaacuccgu uacagagaac     2280

gaaaauacca accuccggau caaagcgcug cuggucgagc uuaaggaaga uacaaauauu     2340

gagaacguga gaccccacag cccuaugcag caggagaucc ucaaaaucua ugaagagggc     2400

gugcugaaug ccgucgaaaa cauugacgag gauauccuga agauuuccaa gaccgcucaa     2460

cccucuaaaa gcgaacucau ccgcuacaag cuguggcuug agcagaaaua ucaguccccc     2520

uacacggggg agaugauucc ucugucuaga cucuuuacaa gcgacuauga aaucgagcau     2580

gugauuccgc agucccggua cuucgaugac agccugucua auaagguuau cugcgaagcc     2640

gccgugaaca aacgcaagga uaaucaacug ggacuugagu uuaucaagaa ccaccauucc     2700

gaaaagauug agcugggcaa ugggagaacc gcucaggucc ucgaggugga agccuacgag     2760

agcuucguua agaaaaacua ugacaagaau cggggcaaac gcaacaagcu gcuccuggaa     2820

gaugugcccg agaagaugau cgagagacag cuuaaugaca cccgguacau uucuaaauuu     2880

gucacacauc ugcucuccaa caucgugcgc aaggacgaag uuaacagcaa agaugacgga     2940

auuaauucua agaacaucau cccuggcaau gggaaaauua ccagccaacu gaagcaggau     3000

uggggacuga acgacgugug gaaugagcuu auccugccca gauucgaacg gaugaacaau     3060

cucacaaacu ccacggauuu caccagcaca aauaccaacg gcaagauuau cccuacaguc     3120

ccccuggagu auucuaaagg guuuuccaag aaacgcauug accacagaca ccacgcgcuc     3180

gaugcucugg ugaucgcccu ugccacccgg gaccauguga aucugcucaa caaugaaagc     3240

gcucugccga agaaagauag aacgaaggag gagcagaagu ccuuccgcuu ugaccugcag     3300

gucaaccuuc ggaauaaaga aaaguacuuc aacguuaaag agggaagaga aaaggagaaa     3360

uuuaccacau ucaagaaacc uuggaauacc cugacacaag auacccgcga cgcccucgaa     3420

aacauggugg ugucuuuuaa gcagaaccug cgggucauca auaaggcuac gaacuauuac     3480

gagagcuaua cagacgagaa uggcaaccuc agacuggaua aauacgggaa ucccaagaaa     3540

gaacuuauuu cccagaaggg caccaacugg gccauccgca aaucucugca caaggagaca     3600

gugagcggac agauucaccu ggaccggguu aaggugacca aaggcaagau cgucaccgcg     3660

acaagaaaau cccucgauuc uagcuucaau gaaaagucca uugagagcau cacggacacc     3720

gggauucaga aaauccugcg caacuaccuu aaggccaagg auaauaaacc ugacguggcu     3780

uuuucucccg aaggaaucga ggagcugaac aaguccauua gcguuuauaa ugaaggcaaa     3840

aaccaucaac cuaucauuaa ggugcggauc uucgagcagg ggucuaaauu ucccgucgga     3900

gaaacaggca auaagauugc caaguucgug gaggcugcca aagggaccaa ccucuucuuu     3960

ggcaucuacc aggauaagac aggaaaaaga uccuaugaca ccaucccgcu gaaucucguu     4020

auugagcggc ugaagcaagg ccuuagcccu gugcccgaaa cgaacgagaa agggcaccag     4080

cugcuguucc aucucucucc caacgaucug gucuacgugc cuacaaucga cgauaaugaa     4140

cagcuugacu ccaaccgcgu guauaaguuu accgauagcu ccgacaccac auguaauuuc     4200

auuccgagca acaucucugc gauuaucuuu aauaucaaca gcaaagagca ggaaaagaga     4260

ggaauuaauu accccaucca aaacgagcug ggcgucgggu ccccucaguc uaagaaucag     4320

aaauccuucg acggauuuau gauuaaggag guuugcauca aacucaaggu ggaucggcug     4380

ggcaacauua gcaaaaccug a                                               4401


<210>  4551
<211>  4416
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4551
augugcaagu acuucuauau caaaaacuuu auuaccuucg ugcugagcaa guccaucauu       60

cccaugaaac acauccucgg ccuggaccuu gggucuaaua gcauuggaug ggccuuuguc      120

cagcaagauu ucgagaacaa gcagggcaaa aucaucgcua caggguccag aauuaucccu      180

auggaccagg gcauucuggg agauuuugaa cgggggaaua cgguuucuca gaccgccgag      240

cgcacaaccu acagaagcac acggcgccuc agagaacggc aucugcuucg cagagagcgg      300

cugcaccgcg ugcuccauau ccugggauuc cugccgcccc acuaugacgc gcaaauugau      360

uuuaccaaga gauacggcaa guucaucgac aacgcugaac cuaaaauugc cuauaauaac      420

ggcaauuuua ucuucaugaa cuccuuuaau gagaugaucg aggauuucaa gaaacaucag      480

ccccagcucu uuuacaagaa aucuaacggg gaagagagca agauuccuua ugacuggaca      540

aucuacuacc ugcggaagaa agcccuuucc cagaagauua cccaacagga acuggcuugg      600

cucauccugc acuucaauca gaaacgcgga uauuaccagc uuagaggcga ggaggaaacg      660

gagaacccca auaaggaagu ggccuuccac agccugaaag ucguggaugu ugaggcugaa      720

gccccuaaca agaaagggga gauuugguau accauccggc ucgagaaugg auggaucuac      780

cgcagaacau cuaagauucc gcuggacgau uggaagggca aaacccggga cuuuaucgug      840

acaaccgauc ugaacgacga cgggacgauu aaggucgaua aagaaggaaa ggagaaaaga      900

uccuuccgcg cgcccgacga aaacgauugg aaucuccuga agaagaaaac agagcaugac      960

aucgauuuua gcggcaagac cgugggcgcc uauauuuacg accaccuucu gcaaaacccu     1020

aaucagaaaa ucaaggggaa acucguucgg acaaucgaaa gaaaguauua caaggaugag     1080

cugaaagcua uucuugccaa gcagaucgcu cugcaacccg aacucuucac cgaaacccug     1140

cuggccgacu guauucgcga gcucuaccgg aaaaacgaaa cacagcagca gaaucugcuu     1200

ucuaaggauu uucugcaucu cuucguggag gacaucauuu ucuaucaaag accucugcgc     1260

uccaaaaaga gcacgaucgc gaacugcacc cuugaaaagc ggucuuacau ugauaaagag     1320

acaaaugaga gaaaggacgc ccccaucaaa gucugugcua aguccaaccc guauuaccag     1380

gaauuucgcg ugcugcagug gcuccagaau cugcggaucu augagauuga aaccgacaga     1440

gaggugacag ccgaguuuau cgaaaccccu caagauugcg aggcucuguu caacuuucuc     1500

augagccaga aagaaauuga caauaaggga cugcuuaaau acuuccuguu ugccaagaac     1560

ccccagauca aggauaaagc cgcuaagucc gagcucaaaa aguggcugac gacauaucgc     1620

uggaauuacg ucuacgacgc ggccgauaac ucuagcaaaa aguauccuau gaaugaaacc     1680

ggcuacgagc ugcagcggua ucuucaaaag guugaggggc uccccgcuaa cuuccugacc     1740

uucgacauua cauaccagcu guggcaccuc cuguauuccg ugaaagauaa gguggaauuu     1800

gagaaagccc uuaagaaauu cgccaagaag aaacagcugg acaagaccag cuuugucgau     1860

ucuuucaaaa acuuuaagcc cuacccuaau gaauauggau ccuucagcga gaaagcuauc     1920

aagaagcucc ugccgcugau gagauuuggc aaccacuggg acuucaaucg gaucgaugug     1980

ucuacacaga aacgcauuga cgaucuuauc aacgggguug ccaaugaaga cauuagaacg     2040

aucauucggg agaaggcgga gaaauaccaa cucaacagcg aaaccuguuu ucaggaccug     2100

ccccuguggc ucgcucagua cguggucuau aauagacaug ccgaggccac agauaucgaa     2160

aaguggaccu ccgugucuga ccuggaggcu uaccuugaag auuucaaaca acacagccug     2220

cgcaacccua ucguugaaca gguggucaca gagacccucc ggguggucag agacauuugg     2280

cagcaguaug gaggcggcca agccaauuuc uucgaugaaa uccauauuga gcuggggcgc     2340

gaacugaaga aaacggcuga ggagagaaag cagcuuuccg acaagaacca ggaaaaugag     2400

agcaccaacc uccggaucaa aaagcugcug gccgaacuca aagaggaugc gucuauucag     2460

aaugugcgcc ccuacucccu gguucaacag gaacuucuga agaucuauga ggagggagug     2520

uuuagcgcca cagacgaagu cgaggaagau auccagaaaa uuagaaagaa ggcugagccu     2580

ucuaaagccg accuccagcg guacaagcug uggcuggaac aaaaauauaa gucccccuac     2640

accggcgagg ugaucagccu uacaaaacuc uucaccgagg aauacgagau ugaacacauc     2700

auuccgcagu cccgcuauua cgaugacucu cugagcaaca agguuaucug cgaguccgcu     2760

gugaauaagc ugaaaacgaa ccagcucggg cuggaauuua uucaaaacca ucacggagag     2820

aaggucagaa ucacagccaa uaaagaggug accaucuuua cagaggagca guauaaggag     2880

uucgugaaaa agcacuacgc gaacaauucu gccaagcagc agaaacuucu gcucaacgag     2940

auuccugaga agaugguugc ucggcaacug aaugauaccc gcuauaucag caaauacauu     3000

uccgaaaucc ugagcaagau ugucagaucu gacgagaaag acgaaggcgu gaacuccaag     3060

aauaucaucc ccuguaccgg gaagauuaca acggcccuua aacaggauug gggccugaac     3120

gacgugugga auaagcucau ccugccucgg uuugagcgca ugaaccagcu cacccagaca     3180

caacuguuca ccagcuauaa ugauagacuu cagaaaaacc ugcccacagu cccuauugaa     3240

uacucucagg gauuucagaa gaaacggauc gaccauagac accaugcuau ggaugcccuc     3300

gugauugccu gcgcuacccg cgagcacauc aauuacauga acaaucaaca cgcgcuggac     3360

aaguccaaag auaagaagga gaaacagcag agccggaucg accugagaac gauucuuugu     3420

gaaaagaaaa acaacaauug gcaguucaag aaacccuggu cuacauuuac ccaagaggcc     3480

aaggaagcuc uggagcauau cguugugagc uucaagcaga accuccgcgu cauuaauaaa     3540

gccaccaacu auuacgaaaa gugggaggag aaaaauggcg ugcugguuaa gaaaaaggug     3600

aagcaggaag ggacaaacug ggccauccgg aaaccgcucc acaaggagac cgucuccgga     3660

aaaauugugc uggauagaaa gaucggcaaa gacaagauuc uuacagcuac gcgcaaggcc     3720

guugauauca ccuuuacaga gaaaaucauu agcucuauca ccgacacagg gauucaaaag     3780

auccugcuca auuaucugaa auacaaggga uccccugaag uggcguucag caccgaaggc     3840

cuggaggagc uuaacaaaaa uauugcucag uauaacaaug ggaagaaaca ucagcccauc     3900

uacaaggucc ggaucuuuga agagggcucc aaguuccccc ugggagaaac gggcgccaaa     3960

gccaccaagu auguggaggc ugccaaaggg acaaaccucu ucuuuggagu guaccagggc     4020

aaggggaaaa gaaccuacgc gacaauuccu cugaaugagg ucaucgaacg ccaaaagcag     4080

ggacucccgu cuguucccga gcggaacgaa aagggcgagc cucugcuuuu cagccugucc     4140

cccaaugacc ugguguaugu gccuauggaa ggggagauug cugagaccau cgauuuuaac     4200

aaucucucua aagaacagaa ggagagaauu uacaaaacag ucagcuucac cggcaaccag     4260

ugcuuuuucg ugcaagaagc cguugccacg cccaucguga acaagaugga guauuccccu     4320

cugaauaaaa uggaacggga cauccuugga acaaugauua aggaggucug uaucaagcug     4380

aaaguggauc gccucggcaa cauuaccaag gcuuga                               4416


<210>  4552
<211>  4416
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4552
augggcuaca aggcccccag aaacgacaaa ggggagaucg auuauggagu gggccuggac       60

cucgggaaug gaagcgucgg cuggguugcu auggauaagg acuaccaccu gauucgggcc      120

cgcgggcaug aacuuaucgg agcgagacug uucgauccug cuaacuccgc cgagaaucgg      180

cgcgaaucua gaaccacacg gcgcagacuc agccggcgca gauggcggcu gcgcaugcuu      240

gacucccugu uugccucuga gcucgcuaaa auugauccga acuuccuggc cagacggaag      300

uauagcuggg ugcaccccaa agacgaacag aaucaugaga acugguacgg cuccaucauu      360

uuuggcucua aggagcaaga uaagcaguuc uauaaagacu acccuacgau cuaucaucug      420

cgcaagaaac ucaccgaaga uacagagaga cacgacaucc gggaaauuua cguggcuauc      480

caccacauug ucaaguacag agggcauuuu cugcgcgagg gagagaucaa uagcgaaaac      540

cuuuucgauc ccgagggcuu uguggaacug auuaaagacg uuaucaagcu caauaacucc      600

uaugacgaga gcaccccuau uuucgaagcc ucucccgcgg auguguccga ggcccugcuu      660

aaugagcaca caagccgguc ugggagaauc gaagacaccc uggauaagcu cgacuuuucc      720

gucagccgcg uggauaaaca ugagcugaag ggaaugcggg acgcugccaa agcuguuauc      780

ggcgccauug uggggaacac agcggauuuc ucuaagaucu uuaaucugga caaccucgau      840

aaagacucca aggauaaucu gaaacuuaag uucgccgaca gcaacuuuga cgaaaaguac      900

ggaggcgucg ugggcuccgg gcugcucgau gagacccaga uugaacuggu ggacagaauc      960

cacgcugucu augaugccgu ugcucuuaaa ggcauucugg gagacgccca cacgaucucu     1020

gaggccaagg uggagagcua cgaaaaacau cgcaagaacu ggaaacucau caaggaggaa     1080

cugcggacca aggagaauaa agaugaagug gaccuguggu auggggaucu cuccggaaga     1140

caguacaaga acccuccgga ccccgagaaa ggcccugaug aggggagcaa gaaagacucu     1200

cccauucgcg auuccgacca aaagcagaau cugaucaaga aagcuaacga uuauuucaca     1260

gaacuuauug acgacagcca gcugucugag gaacagaaga auugccucaa aaacgauauc     1320

aauaacggac aacuguuccc uguccagcgg agcuccgaca auggcgugau ucccuaccag     1380

auccaccuua acgagcugag acgcaucauu gagaaucaga agcaauacua uccguuucuc     1440

gcggaaaccu ucauccggaa aacagccgag ggcaagucuu acgaagagaa caagauuguu     1500

gcucugcuga aauuuagagu gccuuauuac gucgggcccc ucgugagcaa ggaagauaug     1560

gaggagcauu ccagcggaaa uucugccuau cacuggaugg cccggaaccc uggccgcggg     1620

gaagcuauca cccccuggaa uuucgacgag guuguggaua aagacgaauc cggagccaga     1680

uuuauuagcc ggcugacggg cacagauacc uaccuucugg gggagcccac acucccucag     1740

cauucucugc uguaugaaga guacaugguc cuuuccgagc ucaacaacgu gagacugaau     1800

guccgccagg gcaaccacug gagcgacaag aaucggcaaa gacuguccua caaacagaag     1860

gaguuccuca ucgaaaaccu guuuaaagaa cacaagaccg ugacccgcaa gagagcggag     1920

gauugucuuu cuagcauggg augcggcgaa guucagcugu ucgggcucuc cgacgagaaa     1980

aaguuugcuu cuagccuguc cagcuauauu acacugaaaa agauccuugg aaaagccuuc     2040

guggaugacu cuggcaauuc cgccauccuc gagcggauua ucgaagucca gacgguguuu     2100

gaggaucgcg aaccgcugac ccaucaacug agccucauug acucucugac aguugagcag     2160

agaaagcagc uugcuaagau ccacuacacc ggguggggaa aacugucccg gaagcuccug     2220

acaagcaaaa agggcucuua uaaaauucau agccagaacg agaucaugcg cacccaacac     2280

uccaucauug acaucaugag aggggauagc cugaaucuua uugaaaucau ucgggacaag     2340

aagaucggca ucggagagug gauugauaac gaaaaucucg accgcgagac gggcgaaucu     2400

gaugugucca gacuggagag cgucuucgag gggaucguga ugucuccccg ggugaaacgc     2460

ggaguugucc aggccgugag agugauugac gaucuggcua aggccaucgg caacccuccc     2520

cagcggauuu uuaucgaaau ggcggacgag guccaggccu cccaaagaac agugagccgc     2580

cucucccggc ugaaagcucu uuacgggucu ccuagccugc agaaggaguu caaugaucuc     2640

aaagaccuga gaaagucccu gaacgaaacc aaggaugccg accuucagga agaccgccuc     2700

uaucuguacu auauccagcu gggaaaagau cucuacaccg gcaaggagau uucuaucgac     2760

agccugucca gcgcuuacga uauugaccac aucguucccc aagcccuuac acaggaugac     2820

ucuauugaua aucgggugcu ggucuccaga aaagagaacg cgcgcaagac cgacagcuau     2880

cucuacacac cggaucugau caaaaauaug cggccucugu gggggaaacu ucuggccgcu     2940

ggccucaugu cucagaaaaa guaugacgcc cugacgagag uggaugacuu caaggaucgc     3000

gaaaaacaac gguuuguuca gagaucccuc guggagaccc ggcagauuau gaagaacguc     3060

agcacaaucc ugcgcaccua cuauggagaa gacacaaacg ugguuacccu uaaaucuucc     3120

aucacgcaug acaugagaaa guaccugggc uuccccuaua aaaaucggga uauuaacgac     3180

uaccaccaug cucaggaugc ccucuguauc agcgccgcug ggcaauuuau gcugaaucgc     3240

cacuucgugu ccggaggcga ggucaccgac ggggcgagca acgccuacaa uauguaucug     3300

gcugaauacc uucaggaguu uagagaugcc aaggcccggg gagagagaaa gcgcucuaca     3360

gcuuucggcu uugugguggg gagcaugucc ucuaaagacg augaaaagcg gaccgaccau     3420

agaacaggcg agauugucug gagcgaaacc aacgccgagu aucugcgcaa aguuaugaau     3480

uacaagaaaa ugcucaucac gcagaaggug ggagauguga agacacgggc gcuguaugac     3540

gagaccagau acggccacaa agaauccucu gcuaagagca uuaaauucga uaaccuccgc     3600

cgggacacau cccuguaugg gggauuuucu ggcgccagca ccgccuacau gaugcuuguc     3660

gaguacaaga aaaagaauaa gcuggugucc aucaccgcuc aggaagccag ccuccuggcg     3720

guugcugauc aaaagaacga cacagugucu gucauggacg ggauccuggc cucccacgga     3780

cuuagcggcg ccaagauucu gcucccuaaa aucgugacgg gccagcugcu cgauuauucu     3840

gacgcucugg uuacccuuca guccgccagc gagcugaaua cagcuagaca gauguggcug     3900

ccccgcaacg auuacaauga cauugauauc auucucaagu ccaaagacau cgaagaugcc     3960

gcgcauaagc ugggguauga cucugauacc gugcacgucc uuagacggau ccaaaagcug     4020

uucgaggccg uguuucgcgu ugcugagagc caguucccuc uccaucagcu guccaaagac     4080

caacaggaag agauucucag agauccccag aacuacggau uuggcagcgc cgugaagcug     4140

cuugggggac ugcugaaagc ucuccacgcc aaugccgaaa acacaaaccu ggcuggccuu     4200

gucaaggugc ugcaugggga caaagaguau ucugacgaug gaaaggcgaa agaguucaag     4260

aaguucggcu uuuccccgcg guggcgcaga aucagcaauc cuaccggcuu cacgcucucu     4320

gacagcgaua cauuuauuua ccaguccccc ucugggaucu ucgaacaacg ggugaccguc     4380

aaagagcuga aggaaaaagc cgacaagcag aaauga                               4416


<210>  4553
<211>  4422
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4553
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugggc ccuuaucgag       60

aaagauacag accagaagaa cggcaauauu cuggggacgg gauccagaau cauucccaug      120

ucucaagaua ucaaagacga auuuggcaag gggaacagca ucucccagac cgcugaucgg      180

acaaaauuuc gcucugugag acggcuccgc gagagacauc ugcuucggcg cgaaagacug      240

caccgggucc ucaauguucu gggauuccug ccuaagcacu acgccgacca gauugauuuu      300

aagaaacgcc ucggcaagcu ggugaacgag accgaaccga aacuugugua ugacaauaag      360

aacuucaucu uuaaaaagag cuucgaggcg augcuguccg auuuuaauca gcaucaaccc      420

cuccugcuug cugacggcaa gaaagucccu uucgauugga caauuuacua ucugagaaag      480

aaagcccuca ccgagaagau cgaaaaagag gaacuggccu ggcugauucu caacuuuaau      540

cagaagcggg gguacuauca gcugcgcgga gaggaggaag aggaaaaccc caauaaaaug      600

guggcuuucc acucucuuaa gaucguugag gugauugccg acgaaagcca gaagggcaaa      660

gcugagacau gguacucccu gauccucgag aacgggugga ucuauagacg gagcucuaag      720

accccucugu acgauuggaa aggaaagcaa cgcgacuuua uugucacgac cgaccuuaau      780

gaugacggcu ccaucaaaac agauaaggaa gggcuggaga agcggagcuu cagagccccc      840

guggaagacg auuggacccu cguuaaaaag aaaacagagc aggaaauuaa cagaucucau      900

aagaccgugg gaacguacau cuaugagaac cugcugcagg acccuaaaca gaagauuaag      960

ggcaaacucg uccgcacaau cgagcgggaa uuuuacaagg augagcugaa aaugauccuu     1020

aagaaucaac agaaauucca cccggaacug aacgacgagc accucuauau uaauugcgug     1080

agagaacugu accgcaacaa ucaggcgcau caaaccaucc uuuccaagaa ggauuucgug     1140

caccuguuua uugacgauau cauuuucuau cagcggcccc ucagaagcca gaaaucuucc     1200

aucagcaacu guacacugga guuucgcaag uacaaaaaug agaacggcac cgaccagacc     1260

gacuaucuga aggccauccc uaaaucccau ccccucuucc aagaauuucg gcuguggcag     1320

uggauuuaca aucuugggau cuaccagaag gaggaugaca caaacgucac ggaugaacug     1380

auuaaaaaua ucgacggaua ucaggaucuc uucgacuuuc ugaacaauaa gaaggagauu     1440

agacaagaua uccugauuaa auuccuuguu gagcagaccg gccucaaggg gaaaccucug     1500

ucuagcgugg uggcuaagua ccgcuggaac uuugucgaag acaaaaucua ucccugcaau     1560

gagacaggaa cccagauccg gacaagacug gccaaggugg aaaacacccc ggaggauuuc     1620

cucacggcug aaacagagau uaagcugugg cacaucauuu acuccguuaa cgacaaagcc     1680

gauuaugaga aggcgcuuaa aaccuuugcc gaaaagcaca aucuggacac ccauagcuuc     1740

guggagaacu uuaaaaaguu cccucccuuc aagucugaau acggcuccuu uagcgagaaa     1800

gcuaucaaga aacuccugcc ucugauucgc cuugggaagu auuggucuug ggaaaauauc     1860

gauaaaaaga cacaggagcg gaucgagaag auucucaccg gagaguucga cgaagccauc     1920

aaaagcagag uccgggacaa ggcuauucac cugacagaag aggaucauuu ucaaggccug     1980

cagcucuggc uggccuccua cgugguuuac ggccgccacu cugaagccga caaccuugau     2040

aaauggcaua cgguggcuga ccuggaggau uaucucgcgg aguucagaca gcacagccug     2100

cggaauccca ucgucgaaca ggugauuacc gagacacuga gagucgugaa ggacaucugg     2160

cuucaauacg ggcagggagc caaagauuuc uucuccgaaa uccacauuga gcucgcucgc     2220

gagaugaaga acaccggcga cgaacggaaa agacugacaa gccugguuac cgauaaugag     2280

aacacgaauc uccgcaucaa ggugcugcuu ucugaacugc ucaacgacgg gaaugucgag     2340

aacgugagac ccuauucccc uaugcagcaa gaagcccuga agauuuuuga ggauggaguu     2400

cugaauagcg acaucgagau ugaagaugac auccuuaaaa ucucuaagau ggcccagccg     2460

accaacacag agcucaaacg guacaagcug uggcuggaac agaaauaucg cucccccuac     2520

accggccaga uuaucccucu caauagacug uucacagcug acuaugagau ugaacauauc     2580

auuccccaaa gccgguuuuu cgaugacucc uuuucuaaca agaucauuug ugagagcgcc     2640

gugaaccagc uuaaggauaa acagcugggg cucgaguuca ucaagaauca cuccggcacc     2700

aucgucaaac ugggaauggg caagacggcg aaaauucugg acguggaauc uuacaacgag     2760

uuugugaagg ggaauuacac aaagaacauc agcaaacgca auaaacuuau gcuggaagag     2820

auuccugaua aaaugaucga aagacagcuc aacgacaccc gguauauuuc caaguucauc     2880

aucagcgcuc ugucuaauau uguucgcucc gagagcaacg augacggauu caauucuaaa     2940

aacguccucc ccggcaaugg gaagaucaca uccagacuga agcaagauug gggaaugaac     3000

gacauuugga augagcuuau ccugccgcgg uuugaacgca ugaacaaaau uaccgauagc     3060

gccguguuca ccgccuggaa ugagaacaac cagaaguuuc ucccuaucgu gccccuggag     3120

uucucuaaag gcuuucagaa gaaaagaauc gaccaucggc accacgcucu ggacgcccuu     3180

gucauugcug ccagcacaag agaacauauc aaucugcuca acaaugagca cgcgaagucc     3240

gauaagcgcu ucgaccugaa ccggaaacuc agaaaguuug aaaaaguggc cuacaaugau     3300

ccuaagacgg ggaaacgcau ugagaaggaa guucccacca ucuuccugaa accuugggag     3360

acauuuaccg uggacgcuaa gaacagccuu gaaucuauua ucgucuccuu caagcagaau     3420

cugcggguga uuaacagagc cacaaauuau agcgaguccu acaacgauga gaauggcgcu     3480

cuccgccugg gaaaagacgg caacccccug aaggaaaaga ucaagcaauc uaaaggggau     3540

agcugggcca uccggaagcc gaugcauaag gacuuuguuu auggaaaagu gcaccuucag     3600

aaugucaagc ugccuaaaga uaaguucauu accgcgauca gaaaaucccu cgacgugacg     3660

uucgaucuga agcgcauuga gacaaucacc gacaccggca uucagaagau ccucaaaaac     3720

uaccugcauu cuaaggaaaa uaaaguugag cuugccuuua gccccgaagg gaucgaggau     3780

augaaccaac acauugagcg guauaaugac ggaaagaaac accagcccau ccauaaggug     3840

agagucuucg aaauuggcuc ccgguuuucu cugggggaga gcggcaacaa gaaaacaaag     3900

uucguggaag cugccaaagg aaccaaccug uuuuucgcug uguaccagga cgauaauaag     3960

ggcaaacgca acuacgagac aaucccucuc aauauuguca ucgaaagaca gaagcugggg     4020

cuucacuccg uuccgacgga ggacgccaaa ggaggcaagc ugcucuuuca ucugagcccc     4080

aacgauaugg uguaugugcc uaccucugag gaacucgagg acaaucuggc cuccgucaac     4140

aucaagcggc uuaauacagc ucaacgcaaa agaauuuaca agguggaaaa agcgagcggg     4200

guugagugcu auuucauccg gcaggauauu gccuaccuga ucaagaacua ugacgcuaaa     4260

accaagauug gagagcugga aucucagaau aagcuccaga caaccaugac ggaugacaga     4320

cugaaaauca ccgaugugug ugucaaacuu aacauugacc gccugggcuc caucgccgag     4380

acauuucucg ggaaucaaua cggcgccagc aagaagaccu ga                        4422


<210>  4554
<211>  4422
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4554
auggccaaga uccugggccu cgaccugggg accaacagca uuggaugggc uguggucgag       60

agagaaaaua ucgauuucuc ccuuauugac aaaggcguuc ggaucuuuuc ugagggggug      120

aaaagcgaaa aaggaauuga guccucucgc gccgcggaaa gaacaggcua ccggagcgcu      180

cgcaagauca aauauagacg gaagcugcgc aaguacgaga cgcucaaagu gcugucccuu      240

aacagaaugu gcccccuguc uaucgaggaa gucgaggaau ggaagaaaag cggguucaag      300

gauuauccuc ucaauccgga guuucugaaa uggcugucca ccgacgagga aagcaacgug      360

aaucccuacu ucuuucggga ucgcgccucu aagcacaagg uuucccucuu cgagcugggc      420

agagccuuuu aucauauugc ucagcggcgc ggauuccuua gcaacagacu ggaccaaucu      480

gccgaaggca uccucgagga acacuguccu aaaauugagg cuaucgugga ggaucugauu      540

uccaucgacg aaauuagcac aaauaucacc gauuacuuuu ucgagacagg gauccuugac      600

ucuaacgaaa agaauggaua ugccaaagau cuggacgagg gcgauaagaa acucgucucc      660

cuguacaaga gccugcucgc gauucugaaa aagaacgaau ccgacuuuga gaauugcaag      720

ucugagauca uugaacggcu uaacaaaaag gacgugcugg ggaaaguuaa gggaaaaauc      780

aaggauauua gccaggccau gcucgacggc aauuacaaga cccuggggca guauuucuac      840

ucccuuuaua gcaaagagaa gauccgcaac caguacacau cuagagagga acauuaucug      900

uccgaauuua ucaccauuug uaaagugcaa ggaaucgauc agauuaauga ggaggaaaag      960

aucaacgaga aaaaguucga cggccucgcu aaggaucugu acaaagccau uuucuuucag     1020

cggccccuua agagccagaa aggccucauc gggaagugcu cuuucgaaaa gagcaagucc     1080

agaugugcua ucucucaccc ugacuuugag gaguaucgca uguggacgua ccugaacacc     1140

auuaagaucg gaacacaaag cgauaaaaag cugcgguucc ugacccagga cgaaaaacuc     1200

aagcuggucc ccaaauuuua cagaaagaau gauuucaacu uugacgugcu ugccaaagag     1260

cugauugaaa agggcuccag cuucggguuu uauaagucuu ccaaaaagaa ugauuucuuu     1320

uacugguuca acuauaaacc uacagacacc guggcggccu gccaggucgc ugccagccuc     1380

aagaaugcua ucggagagga cuggaaaacg aagucuuuua aguaccagac aauuaacucc     1440

aauaaagaac aaguuagccg caccguggau uauaaggacc uguggcaucu gcucacagug     1500

gccaccuccg augucuaccu guaugaguuc gccaucgaca aacuuggccu ggaugagaag     1560

aacgcuaaag cguucucuaa gaccaagcuc aaaaaggacu uugccagccu gucccugucu     1620

gcuauuaaua aaauccuucc guaccucaag gaagggcugc uguacagcca cgccguguuc     1680

guugccaaca ucgagaauau uguggaugaa aacaucugga aagacgagaa gcagcgggau     1740

uauauuaaga cacagaucuc cgaaauuauc gagaauuaca cgcucgagaa aagcagauuu     1800

gaaaucauua acggacugcu uaaggaguau aaaucugaaa augaggacgg caagcgcguc     1860

uacuauucca aagaagcuga gcagagcuuc gagaacgauc ugaagaaaaa gcucgugcug     1920

uuuuacaagu cuaacgaaau cgagaauaaa gaacaacagg agaccauuuu caacgagcug     1980

cuucccaucu uuauucagca acucaaggac uaugaguuca ucaaaaucca gcggcuggau     2040

cagaagguuc ugauuuuucu caaaggcaag aaugaaacag ggcagaucuu cuguaccgaa     2100

gagaagggaa cagccgaaga gaaagagaag aaaauuaaga acagacugaa aaagcuguac     2160

cacccuuccg acaucgaaaa guuuaaaaag aaaauuauca aggacgaguu cggcaaugag     2220

aaaaucgugc uugggagccc ccucaccccu ucuauuaaga accccauggc gaugcgcgcu     2280

cugcaucaac ugcggaaggu ccuuaaugcc cucauccugg aaggacagau ugaugagaaa     2340

acgaucauuc acaucgaaau ggccagagag cugaacgacg cuaauaagcg caaaggcauu     2400

caggauuacc agaacgacaa uaagaaauuu cgggaagaug ccaucaagga gaucaagaaa     2460

cucuauuucg aagacugcaa gaaagaggug gaaccgacag aggaugacau ucugagauac     2520

caacuuugga uggagcagaa ccggagcgaa aucuaugagg aagggaagaa uauuuccauc     2580

ugugauauua ucggcagcaa cccugcuuac gacaucgagc auaccauucc ccgcucuaga     2640

ucccaggaua auagccagau gaacaaaacc cugugcuccc aacgguucaa cagagagguc     2700

aagaaacagu cuaugccuau cgaacucaau aaccaccugg agauucugcc ccgcaucgcc     2760

cacuggaagg aagaggcgga caaucuuaca cgggaaauug agaucaucag cagauccauu     2820

aaggccgcug ccaccaaaga gaucaaggau aaaaagauuc gcagacggca uuaucucaca     2880

cugaaacgcg acuaccugca gggaaaguau gacagauuua ucugggagga acccaaggug     2940

ggcuucaaaa acucucaaau uccugauacg gggaucauua ccaaguacgc ucaggccuac     3000

cucaaaagcu auuuuaagaa aguugaaucc gugaagggag gcauggucgc ggaguuccgg     3060

aagaucuggg ggauccagga aucuuuuauu gacgagaaug gaaugaaaca cuacaaggug     3120

aaagaucgca gcaagcauac acaccauacc aucgacgcca uuacaaucgc uuguaugacc     3180

aaagagaagu augauguucu ggcccacgcu uggacgcuug aagaccagca aaacaagaaa     3240

gaggccagau ccauuaucga agccagcaag ccguggaaaa ccuucaagga ggaucugcuc     3300

aaaaucgaag aggagauucu ggugucucac uacacacccg acaaugucaa gaaacaggcu     3360

aagaagaucg ugcgggugcg cggcaaaaag caguuuguug cggaagucga gagagaugug     3420

aacgggaaag ccgugccuaa gaaagcugcc uccggcaaga ccauuuauaa gcuggacgga     3480

gaaggcaaaa agcugccccg gcuucagcaa ggggauacaa uccgcggaag ccuccaucag     3540

gacucuauuu acggcgccau caaaaauccu cugaacaccg acgagaucaa guaugucauu     3600

agaaaagauc ucgaguccau caaggggagc gacguggaau cuauuguuga ugaggugguc     3660

aaggagaaaa ucaaggaagc uauugccaau aaagugcugc uucuguccag caacgcgcag     3720

cagaagaaua aacucguugg aacggugugg augaacgaag agaagcggau cgcuauuaau     3780

aaggucagaa ucuacgccaa cuccgugaaa aacccccugc acaucaagga gcauagccug     3840

cuuucuaaaa gcaagcacgu ucacaaacaa aagguguacg gccagaauga cgaaaacuau     3900

gccauggcua uuuacgagcu ggaugggaaa cgcgacuucg aacucaucaa uauuuuuaac     3960

cuggccaagc ucaucaagca gggccaagga uucuauccgc ugcauaaaaa gaaagagauu     4020

aagggcaaaa ucgucuuugu gccuaucgaa aagcggaaua agagagaugu gguccuuaaa     4080

cgcgggcagc agguuguguu cuacgacaag gagguggaga accccaaaga uauuuccgaa     4140

aucgucgacu ucaagggacg gauuuauauc auugagggcc ugucuaucca gagaaucgug     4200

cgcccuagcg ggaaaguuga ugaauacgga gugauuaugc uccgguauuu uaaggaggcg     4260

agaaaggcug acgauaucaa acaagacaau uucaagcccg acggcgucuu uaaacugggg     4320

gaaaacaagc cuacacgcaa aaugaaucac aaccaguuca ccgccuuugu ggagggcauu     4380

gauuucaagg uucugcccuc cggaaaguuu gagaaaaucu ga                        4422


<210>  4555
<211>  4425
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4555
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuaucgag       60

uccgauuucg aaaagaaaca gggcaagauu aaugggcugg gaucuagaau cauucccaug      120

agccaagaca uccucggcaa auuugauucc ggggugucua ucagccagac agcugagcgg      180

acguccuacc gcggagucag acggcugaac cagcgcaauc uucugagacg ggaacgccuc      240

cacagaguuc ugaacauucu ggacuuccuc ccuaagcauu auucuaaugc caucgauuuu      300

gagaagaaac ugggccaguu caaggaaaac guggaggaga aacuuaauua cgcgaagaac      360

caaauuggcc ggcacgaauu uaucuucaug gacagcuuua aagagauggu gaaggaguuc      420

aagcagaccc agccgcagcu guuuuauaaa cgcgaaaaug ggauugagac aaagaucccc      480

uacgauugga cccucuauua ccugagaaag aaggcucuuu cccaaaagau uaagaaagag      540

gaacuggccu ggaucauccu caacuucaau cagaagcggg gauauuacca gcugcgcggc      600

gaagacgucc uggaugagag caagauugaa gacuuuuacu cucucaaagu gguugaggug      660

caggagacag aagauaagaa cgccagaggg accugguaua augucauccu ggagaacgac      720

uggguguaca aacggcaauc caaggaaagc cuugauaauu ggauugggaa aaacaaggag      780

uucaucguua caaccaacaa ggaaaaagac ggcucucuga agcucgacaa agcugggaau      840

gugaagcgca acuuuagagc cgucgauucc gagaaagacu ggauugcuau caagaagaaa      900

acggagcagg auguggaaau cagcaagcau accgugggac aguauauuua cgagacacug      960

cuucaaaaug ccaaacagaa gaucaaaggc aagcugguca agaccauuga acggaaauuc     1020

uauaaggagg aguucaucaa aauuaucaag gaacagcuca aacaccaucc ugagcugcag     1080

ucuaagacac uguacgaaac cugcauccaa gagcucuauc ccagaaacga agcgcaccag     1140

aauaacauua aauccaaggg cuuugaguac cuguucacgg acgauaucau uuucuaccag     1200

cgcccucuua agagcaaaaa guccacaauc ucuggguguc aguaugagac ccggcuguuc     1260

aaaaagacag uucucaauaa agugaccaac aaggaaaaug agauuuggca aaaggaaccc     1320

cugaaagcca ucagcaaguc ccacccucuu uuucaggagu ucagacugug gcaguuucuc     1380

aaaaaccuga agauuuacca gaaagaagac gugaagggaa aucugaucga ucaugacauc     1440

accagcgauc uccugaagac agaggcugac ugggucgagc uuuucgauuu ucugaacauu     1500

aaaaaggaaa ucgagcaaaa acagauuauc caguucuuua uuaauaacaa gcagaucgaa     1560

aaaggcaaga agaauaacua ucgcuggaac uacguggagg acaaaaagua uccgugcaau     1620

gaaacgaaau cucaacucau cucccggcug aagaaaguua agaaccugga ccuuaaucuc     1680

uucuuuacca gcgagacaga gcugaaacug uggcacauua ucuacucugu gaaggauaag     1740

auucaguaug aaaaagccau caagaacuuc gcuauuaaaa auaacaucga caucgauagc     1800

uucgucgaga auuuuaagag auuucccccu uuuaaaaacg aauacgccgc guacuccgag     1860

aaggccauua agcgccuccu gccccuuaug cggauggggg aauauuggaa ugagucugag     1920

aucaaacagg aagugaagga cagagcuuac agcauuaucg agcgccugaa aggaauugau     1980

uucaacaagg acaaaaucga ugaaaagauc accgacgaug acauuaagaa acaacuccug     2040

aaguccuuua ucgauuucaa agacaagaau ccucugaaag gccuuaacac auaucaggcc     2100

uguuaccucg uuuauaaucg gcauagcgag gugaacucua uugcuuacug gaaguccccc     2160

gaugacauca auaaguaucu ggagggguuu aaacagcaca gccugagaaa cccgauuguc     2220

gaacaggugg ucaccgagac gcuccgcaca gugcgggaca ucuggcaaua cuacggaaag     2280

ggcaaagccu cuuucuuuaa cgaaauucac guugagcugg gcagagaaau gaagaauccu     2340

gccuauaaac ggaaggcuuc caccaaccgc gugaccgaga augagaacac aaaucauaga     2400

aucaaggaaa uccuuaaaga gcugcagaac gaaggggucc aggagguuaa gagcuacucc     2460

cggaaucagc aagaaauucu caaaaucuau gaggagggaa uuuaccaguc uaucgaugug     2520

guggacgaug cgauugaaaa gaucagaaag aacagcuccc ccaccaaggc cgagaucauu     2580

aaauauaagc uguggcugga acagggcuac acaucuccuu auacggggaa gaccaucccc     2640

augagcaaac uuuucacaac cgacuaccag auugagcaca ucauucccca aucccucuac     2700

uuugauaaua gccugucuaa caagaucauc ugcgaauccg aggucaauca gcugaaagac     2760

aaccagacag cuuaugaguu cauuaagaaa cauggaaccg aaaaggugag ccucggcaag     2820

aauaaaacgg uggagaucuu uacccuggaa caauacgaga agcacuguaa ccaguauuuc     2880

aaaaagaaua aaaacaaacu uaagaaacug cucucugagg aaauuccuga gggguucauc     2940

aaccgccagc ugaaugauuc ccgguacauu agcaaguuua ucaaaggccu gcuuggaaac     3000

cucguuagag aagaggacga acaggagguc acaaccaagc auauugugcc ggugacaggc     3060

gccaucaccu cuaaacugaa gcaagauugg gggcugaaug acaaguggaa cgagcucguc     3120

gccagccgcu uccagagacu gaauacgaug acaaacucca auaacuuugg auauugggau     3180

accaaaauca augcuuuccg ggugcagguu cccgacgaaa uuagcaaggg cuuuucuaaa     3240

aagcgcaucg aucacagaca ccaugcccuu gacgcgcugg ugauugcuug cuguacacgg     3300

aaccacauca auuaccucaa cgcccugaau gccgagaaaa agaacuaugg gcugcgcgac     3360

cuucugcuca uuaaaaauga uaagaaccau uacaccaagu ucuuuaaaca cccuugggac     3420

ggauuccccg ucgaagcuaa guccgugcug gagaaaacca ucaucagcuu uaagcagaau     3480

acaagaguua uuaacaaaac gaacaauaag accuggcaau gggccgaaaa ggagggccag     3540

cucaaaaagg ugcuggucaa acagacaaag ggggauaacu gggcuauccg gaaaccuaug     3600

cacaaggaga ccguguccgg caaacuuaau auucccacac cgaaaggaaa gaucgccacc     3660

gcgucucgcg uuagccuguc cgaaauuaaa acgagaaagc agcucgacaa aaucacagau     3720

aucucuauuc aaaagauccu ggagcggcau cugaacaauu accuugacga uaagggcaaa     3780

gaaaaguucg accuggccuu uaacgcugau gggguggagg aacucaauaa aaacauuaag     3840

guccugaaua aaggaaagaa ccaccagccu aucuauaaag ugcgccucua cgagauuggc     3900

agcagauuca aggugggaaa uuccggaaac aagaaagaca aguaugucga ggccgcuaaa     3960

ggcaccaauc uguucuuugc caucuaccuu aacgaaaagg auaaaaagcg gguuuaugag     4020

accauucccc ugaaugaagu gaucgagcau cagaaacagg ugucuaaccu cccuaaaaac     4080

gagagaacac ccgucccugu gaaucacacc aaggggaaau uccuguuuag ccuguccccc     4140

aacgaccuug uuuacgugcc gacagacgaa gagaucgaua auccuagccu ggucaacuuc     4200

aauaaccuca auaagaaaca aguggaaaag auuuauaaga cgguuucuug cuccaccuac     4260

cagugcuuuu ucgugcauaa cacaaucagc aaacccauuc agaauaaggu cgaguuuucu     4320

gcgcugaaca aaauggaaaa guccaucgac ggcauuauga ucaaagaucg cuguuggaag     4380

cucaagaugg accggcuggg aaauaucauu agcgugauca gauga                     4425


<210>  4556
<211>  4443
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4556
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc caaagugugc       60

gucgauaaug acggcaacua ccuuagagau aucaagcugg ggucccggau uauccccaug      120

ucucaggaca cgcucggaaa uuucgauaaa ggcguuaccg acagcccuac agcugcccgc      180

accggguuua gaggagugcg gcgcauuaga gagcgggcgc ugcaacgcag agaacggcuu      240

caccgcgugc ugcaugucau gaaguuccuc ccgccccacu augagaacgc uaucggcugg      300

aauagagaag aggccacaac cuacggcaaa uuucugaacc auggggaacc uaagcuggcc      360

ugggagaagc aggaggaugg auccaugcgg uuccucuuua uggacucuuu ccacgaaaug      420

auggcugauu uugccgagag ccagcccggc cugguggcug ggggaaagaa gaucccucuu      480

gacuggacac uguauuaccu ccgcaaaaag gcccugaccc aggcgauuag aaaagaagaa      540

cuugccugga uccugcucaa uuucaaccaa aagcggggcu auuaccagcu gcgcggggag      600

gaagaugagg aaaaucccgc uaagagagag gaauaucaug agcugaaagu uguguccguc      660

gaggccgcug aagagggaaa gggcggcaac acgugguaca augugacccu cgaaaacggg      720

uggauuuacc ggagacaguc uaaaguugag cugcacgacu gggugggaaa gacacgccag      780

uuuaucguca ccacagaaua ugagaaagau ggcgugacca uuaagaaaga caagaauggg      840

gaggugaagc ggagcuucag agccccuucc gaagacgauu ggggacuucg caaaaagcgg      900

acggagagcc ugcucgaaga cucuggcaaa acagucgggg cguuuaucua cgaucacauu      960

cuguccgagc cgaccgacaa gaucagagga aacuucaucc gcacaauuga acggaaauau     1020

uacaagaagg agcuugaggc cauccugcuc gaacaaacca aacaucacaa ggagcugaaa     1080

gaucagaaua cccugauugc uuguaucaag gaacucuauc agagcaacaa accccaucag     1140

gacucucuga ugaagaagga uaugacauac cuucugauua cggaccucau cuuuuaucaa     1200

agaccucuga aauccaagaa aagccuuauc gccgauugcc ccuacgagua cuaugaguac     1260

accauuaagg aaacaggcga gaucaaacgc cagggcauua aguguaucgc uaagucuaac     1320

ccuuauuacc aggaguuccg gcuguggcaa uucauuauca aucucagacu guuugaccgc     1380

accgaugaca aagagguuac auccgaauau cugagcucca uggaggacua caccaagcuc     1440

uuuacguauc ugaacgaucg gaaagacauc aaucaggaga cacuucugaa ggauuucuuc     1500

aaacagaaga aagugaagau ggggauggaa aaggaguuuc ccauuagaug gaacuacauc     1560

gaagacaaag agaagaaaua cccgugcaau gaaacccggc acgagcuccu ggccgcccug     1620

gauaaggcug gaauugacca cucuuggcuu gaugacaaag agcgcuucua uagacucugg     1680

caucugcugu acagcgugga auccaaggag gaagcggagg gcgcccuccg gaagcugaaa     1740

gauaacgacg auuuugucaa ugcuuuccuu aaggugaaac cuuuuaagaa agacuauggg     1800

gccuacagcg aaaaggccgu uaagaaacug cucgcuauca ugagaauggg aggccugugg     1860

aacgagaccg auauuuguga gcagacacgc aaucggaucc aaucuauuau caagggggaa     1920

aucgacgaga aaauuaagga aagaaugggc cgcaccuucu gccagcuguc cgacuuucag     1980

ggacuucccg aguggcucgc cuguuaugug gucuacggca gacacagcga ggcgacagau     2040

auccagcggu ggacgaaacc ugaaaaccug auggcuuaua uuaauggguu caagcaacau     2100

ucucugcgca accccaucgu ggagcagugc auucucgaaa cccugagaac aguucacgac     2160

aucuggaagg aggccggaca uaucgaugaa auucacgugg agcuuggccg gagcaugaaa     2220

uccaccgccg accagcgcgc uagaaugucu aagagcaucc ugcagaauga gaacacaaau     2280

cuccggauua aaucccugcu gauggaacuu aagaacgcca aagagaucga aaacguccgc     2340

cccuacagcc cuaugcaaca ggagauucuc agaaucuacg aagagggggc ucugcaggag     2400

cugaccaagg aagaugccca guauccggag aucucuaaaa uuucccaaau ggcgcagccc     2460

agcgccaagg aacuccugaa guaccggcuu uggcuggagc agaaauauug uucuccuuac     2520

acgggaaagc ccaucucccu cagcaaacug uuuaccuccg cuuaucaaau ugaacacauc     2580

auuccucagu cucgcuacuu cgacgauagc uucacaaaua aggugaucug cgagaccgag     2640

gucaaccagc ugaaauccaa uaugcuuggc uaugaauuua ucaagaagca uucuggggag     2700

auugugcacu guacaaccau cggcgacguu aaaauucucg augccaagga auacuacaac     2760

uucgugacgg agcauuaugc uaauaacaga cggaaaaagg agaaacugcu gcucgaagac     2820

aucccccagg aguuucugaa uagacaaaug aacgauagcc gcuacauuuc caagaagguc     2880

auggccaucc uuagcaauau ugugcgggaa gaggacgaac aggagacaaa cucuaaauuc     2940

guuguguccu gcagcggagg caucaccgau agacugaagc aggacugggg gcucaaugac     3000

gucuggaacu cuaucgugua uccgcgcuuu gagcggcuga auaaacugac acagaccgaa     3060

gcguucggaa gaugggagaa caaggaaggc aaacaagugu uucagaccuc ccuuccucug     3120

gagcuccagc gcggguucag caagaagcgg auugaucaca gacaccaugc cauggacgcu     3180

cugguuaucg ccugcgcuuc ucgcaauauu aucaacuacc ucaacaauga aagcgccaaa     3240

ucccaggagc aacgggagga ucugagaaag aaacuuugug acaagaaccg gauuauccgc     3300

aaacccuggg aaacauuuac gcaggaugcc agaaccgcuc uggaggacgu cguggugagc     3360

uucaagaauu auguccgggu gaucaacaag gcgacaaauu acuaugaaaa auacgaugag     3420

ucuggaaaga aaggcauggc cgaacagaag gggcaagaca ugugggcuau ucgcaaaccu     3480

augcacaagg agaccguuuu uggccaggug aaccucagac ggaaagucac agugaagcug     3540

aaggaugccc uggagaauau ccccgccauu ugcgacaaag cucuuagaga uuacguuaac     3600

gaccugaucg ccaagaaauu caauaccaag cagaugcucg cgcauuuuaa auccauuaac     3660

uaucgccuga auaugaaaag cgugaacaag gucggaaucu ggguguucuc cgacgaaaaa     3720

gagccuaugg uugcuacgcg gaaggccauc gauauuucuu ucgacgccaa aagaaucgau     3780

acaauuaccg acaccggcau ccagcaaauu cucuguaagu accugaaaag caaggggaau     3840

gaucccaacg uggcuuuuac accggaagga aucgccgaga ugaauaagca cauuaucgac     3900

uauaacaacg gcaaacagca ucagccuauc cuuaaggucc gcguguccga gcccaaaggg     3960

gcgaaguacc aggugggaga aaccggcaau aaaacaaaga aguucgucga ggcucaaaaa     4020

gggacgaacc uguauuuugc cauuuacgaa gauaaggacg gcaaucgguc uuauaacacc     4080

aucccccuca augagguugc cgaaagacug aaacagggac ugagcccugu gccggagaag     4140

aacgagaaag gcauuucccu uaaguucuac cugucuccca augaucucgu guacgucccu     4200

acagaagagg aaaaacugag caacgagugc aagcucucca aggaacgcau cuauaaaaug     4260

gugagcucua ccuccaauga gugucacuuu auucccuaca caaccgcuaa gccuaucuau     4320

gacaaauucg aguacgaagc caagaacaaa auggggcggg cucugacggg agagaugauc     4380

aaggccguuu gcuggaaacu ugaaguggau agacugggca auauucugaa gaucauuaag     4440

uga                                                                   4443


<210>  4557
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4557
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc caaaaucucc       60

auugaugaca auggcaacua ccuuagagau auccagaugg ggucucggau uauccccaug      120

agccaagacg ugcugggaaa uuucgauucc ggcgucacgg agucucagac cgcuguucgc      180

acaggguuua gaggacuccg gcgcaucaga gaacggagcc ugcuucgcag agagcggcug      240

caccgcgugc uccaugugau gggcuuccug ccuccgcacu augacuccgc cauuggcugg      300

aacaaggaag aacccaccac auacgggaaa uuucugaauc auggagaacc uaagcucgcg      360

ugggagagac agggcgaugg gucuaugcgg uuccuguuua uggacagcuu ccacaaaaug      420

auggaggauu uugcucccca ucagccugcc cuugucgccg acggacgcaa gaucccccug      480

gauuggaccc ucuauuaccu gagaaagaaa gcucuuacac aaccuauuaa gaaagaggag      540

cuggccugga uccuccugaa cuucaaucag aagcggggcu auuaccagcu gcgcggggaa      600

gaggaggaag agaacccgac caaaagagaa gaguaucacg aacucaaggu gguuuccgug      660

gaggcugccg aggaaggaaa gggcggcaau acgugguaca acgucaaacu ggagaauggg      720

uggauuuacc ggagaagcuc uaagaucccc cuugacgauu gggugggaaa aacccgcccu      780

uuuauuguua caaccgaaua ugaggcggac ggcaucacac ugaagaaaaa caaggaaggg      840

gaggugaaac gguccuucag agcccccgga gaggacgauu ggggccuccg caagaagcgg      900

accgaaagcc agcuggaggc uucugggaaa acggucggag ccuuuaucua cgaccaccuu      960

cuggcugaac cugccgauaa gauuagaggc aauuucaucc gcacaauuga gcggaaauau     1020

uacaagcaag aacucgcgga cauccugaga gagcagucca aauaucauga ggaacugcag     1080

agcaagaaga uucucgccga uugcgcugag gaacuguacc gcucuaacca gccccaccgg     1140

gacucccuuc ugaaaaagga uaugguguau cuccugcuua ccgacaucau cuuuuaccaa     1200

agaccgcuga aaagcaagaa aucccucauu gccgauuguc cuuacgagua uuacgaguau     1260

guggacaagg aaacaggcga gaucaagcgc caggggauua aaugcgucgc uaagucuaau     1320

cccuacuauc aggaauuucg gcuguggcaa uucguucuga accucagacu guacgaucgg     1380

accgaggaca aagaagugac cgccgaguau cuuccuagca aggaggacua cgcccgccug     1440

uuuguguacc ucaacgauag aaaagaaauc aaucaggaga cacugcugaa ggacuucuuu     1500

aagcucaaaa aggucaaaca gcgguccgaa aaggaguucc ccgugagaug gaacuauauu     1560

gaagauaaag agaagaaaua ccccuguaau gagacgcgcc augaacuccu gauggcucug     1620

gaccgggcgg gaaucgagag aagcuggcuc gaugacaccg cccgccagug cagacugugg     1680

caccuucugu auucuaucga aguuaaggau gaggcugaac gggcccuccg caagcugaaa     1740

gacgaugacg aguuuguguc cgccuuccug aaggucaaac cuuuuaagaa agauuacggc     1800

gcuuauagcg agaaggccgu gaagaaacuu cucgcgguua ugagaauggg gucucacugg     1860

ugugaagacg auauuugcaa ggagacaaaa gaacggaucg agcauauucu gcaaggagac     1920

guggaggaaa agaucaaaga gcgcaugaac cacaccgcuu acagccuggg cgaacucucc     1980

gacuuccagg cccugccgga guggcuugcc uguuaugucg uguacgggag acauucugaa     2040

gcuacagaua uuaagcacug gaagaccccc gagagccuga uggccuacau caauggcuuu     2100

aaacagcauu cccuccggaa cccuauuguc gagcagugca uccuggaaac gcugcgcaca     2160

gugcacgaca ucuggaagga ggcuggacac auugaugaaa uccauguuga gcuuggcaga     2220

agcaugaaau cuaccuccga ccaacgggcc cgcaugaccg aaaauauucu cagaaacgag     2280

aauacaaacc ugcggaucaa gagccugcuc auggagcuga aaaaugaugc ggacauuaag     2340

gaugugagac ccuauucucc uaugcagcag gaaauccuuc gcaucuacga ggaaggggcc     2400

cugcaggagc ucaccaagga agacaacgau uaugaggaga uuuucaaaau cuccaagaug     2460

ccccaaccga gcgcuaaaga acugcugaag uacaaacuuu ggcucgagca gaaguaucgg     2520

uccccuuaca caggaaaacc cauuucucug agcaagcugu uuacguccgc cuaucagauc     2580

gaacacauua ucccucaguc uagauacuuc gacgauagcu uuaauaacaa ggucaucugu     2640

gaguccgagg ugaaugcucu caaaggcaac augcuggggu acgaguucau uaagaaccau     2700

ggaggcgaaa ucguucacug cacccuucug ggggacguga aaguccaaaa ugaaagcgag     2760

uauaaggccu ucgugacaga acacuacgcg aacaauaaau auaagaagga gaaacuccug     2820

auggaggaua uuccccagga cuuucugaac cgccaucuua augacucucg guacaucucc     2880

aaggugguua aaacccugcu cagcaacauu gucagaacag aagaugagau ggaaaccacg     2940

ucuaaguucg ugguguccug uaccggcgga aucacagacc gccugaaaaa ggauuggggc     3000

cucaaugacg ucuggaacag caucguguau ucucgguuug agagacugaa ugaacuuacc     3060

aagagcgaug ccuucgggcg cugggagaac aaagagggaa agcggguuuu ucagacaucc     3120

gugccucugg aacuccaaag aggcuucaau aaaaagcgga uugaccaccg ccaucacgcu     3180

auggaugccc uggucaucgc uugcgccagc agaaacauua ucaauuaccu gaacaaugag     3240

ucugccaacu cccccaaaaa gcgggaagac cuucgccagc agcugugcga uaagaacaga     3300

auuauccgga aaccguggga gagcuuuacc caggacgcua gagcggcccu cgaagaugug     3360

guuguguccu ucaagaauua uguccgcgug auuaacaaag cuacgaauua cuacgagcgg     3420

uaugacgccg acgggaagaa aacaaucacc aagcaaaagu cuagagagau gugggccguu     3480

cgcaaaccua ugcauaagga aacaguguuu ggacacguca accugcggag aaaagcuauc     3540

gugaagcuca aagaugcccu ggagaauauu cccagcaucu guaacaagga acuucgcgac     3600

uacauuaaug gccuggugga gcggaacuuc aauaccaaac agcuccacgc gcauuuuaga     3660

uccaccgauu aucgcuggaa caagcagaau gucgacaagg uugaggugug gagauucucu     3720

gaugaaaaag agccccuggu ggcuacacgg aagccucugg acacgagcuu ugaugccaaa     3780

cgcaucgaca ccauuacaga uaccgggauc cagaagaucc uucugaacua ccucgaagcc     3840

aaaggcggag acccgcugau ugcuuucaca cccgagggca ucgccgaaau gaaucaaaac     3900

auugcgcucu auaaugaugg gaagaugcac cagccuaucc ugaaggucag aauuuccgag     3960

cccaaaggag cuaaguacca gaucggccag accgggaaca aagccgacaa guucguggag     4020

gcccaaaaag gaacgaaccu uuauuuugcu aucuacgaag augaggaccg gaaucgcaca     4080

uacaacacca uuccucugaa ugaaguugcc gagagacuga agcagggccu ucccccugug     4140

cccgaaaaga acgagaaaga ggucccgcug aaguucuauc uuucuccuaa ugaccuggug     4200

uacguuccca ccgaagagga aauccucaug aaagagugca agcugaacaa aaaucggauu     4260

uauaagaugg ugagcuccac agggaaccag uguuucuuca ucccuuuuag agucgcugug     4320

agcauuguua auaagaugga auacucuccc cucaacaaaa uggagaaggc caucuccagc     4380

gagaugauua aagcggugug cuggaagcug gaagucgauc gccuuggcaa uaucauccgg     4440

guggugaaau ga                                                         4452


<210>  4558
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4558
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc caaagugucc       60

cuugaugaca auggcaacua ccugcacgau aucaagcucg ggugcagaau uauccccaug      120

ucucaggacg uccuggauaa uuucggaaaa ggcguuacgg ugagccaaac cuccgugcgg      180

acaggguuuc gcggagucag acggauucgc gagagaucuc uucagcggcg cgaaagacug      240

caucgggugc uccaccgccu gggcuuccug ccucagcauu augacgcugc caucggcugg      300

gauagcgacu gugcgaagac cuacgggaaa uuucucgaua acggagagcc gaagcuggcu      360

ugggaaagac aggaggacgg cuccaugcgg uuccuuuuua ugaauagcuu cuaugaaaug      420

aucgccgauu uuacaagcca ccaacccgcc cugguugcug acgggaagaa aauuccucuc      480

gauuggaccc uguacuaucu ucgcaagaaa gcccugacac aggcuaucag aaaggaggag      540

cucgccugga uucugcuguc cuucaaccag aaacggggau acuaucagcu ccgcggcgaa      600

gaggaagacg agaaucccac caagagagag gaauuuugcg agcugaaggu ggucgcggug      660

gaagccgcug agaaagggaa gggagacgau aucugguaca acguucaucu ugaaaauggc      720

uguauuuacc ggagaagcuc uaaaaucucc cuggacgauu gggugggcaa gacgaaacaa      780

uucauuauca ccacagagua ugagaaggac ggggucaccc cuaaaaagga uaaggacgga      840

aaagaaaagc gcagcuaccg ggcccccaaa gaggaugacu ggggccucag aaagaaacgc      900

acagaaaccg aucuggaggc uucugggaag acggugggag ccuuuaucua ugaacaccuu      960

cuggcggagc cuuucgacaa gauucggggc aacuuuaucc acacaauuga gagaaaauac     1020

uaucgcgccg aacucgaggc uauccugacc gaacagcgga aguaccauga ggaacugaga     1080

uccgagcaga ugcucgccgu gugcgcugag gaacuguauc gcagcaaucu uccgcaccag     1140

caaucucugc ucaaaaagga uauggucuac cugcuucugu ccgaccucau uuucuaccag     1200

cggccccuga aaagcaagaa gucccugauc gccgacuguc cuuaugagua cuauagauac     1260

guugauaaag aaacagggga gaucaagaaa cagggaauua agugcaucgc caaaucuaac     1320

cccuauuacc aagaguuccg ccucuggcag uuugugcuga aucuucggcu guucgacaga     1380

augaccgaua aggaagugac cgcugacuau cucccuacaa aggaggauua cguccggcug     1440

uuugacuacc ugaacaaucg caaagaaauu aaccaggaga cgcuucucaa ggauuucuuu     1500

agacugaaaa aggugaaacu gggccgggaa aaggaguucg agaucagaug gaacuauauu     1560

gaagacaaag agaagaagua ccccuguaau gaaacccgcu augagcuccu gguugcgcuu     1620

gaucgggccg gcaucgacag aagcuggauc gaugaagacg ggcgccagua cagacugugg     1680

caucuccugu auuccauuga ggugaaagcu gaggccgaaa gcgcccugcg gaaacuuaac     1740

ucugaugacg cuuuugucuc cagcuuccuc aaagugaagc cguuuaaaaa ggacuacgga     1800

gccuauucug agaaggcggu uaaaaagcug cuggcuguga ugcgcauggg cagcaugugg     1860

uccgaagcca auaucugcga ggaaacacaa aaccggauua gaucuaucau ucgcggggau     1920

aucgaggaga aaauuaagga aagaauggga cacaccgccc ggagcuucaa ucagaucgac     1980

gauuuucagg cucucccuga guggcuggcc uguuacgucg uguacggccg ccacuccgaa     2040

gucacagaca uccagcauug ggagaccccc gagcaacuuc agagcuauau uaacggguuc     2100

aaacagcacu cucugagaaa uccuaucgug gaacagugca uucucgagac gcugcggaca     2160

guucaugaua ucuggaagga agcuggccac auugacgaga uccaugugga acugggacgc     2220

gauaugaaga gaaccgccga ccaacgggcg agaaugacca aaaacauccu uaagaaugag     2280

aacacaaauc uccgcauuaa aucccugcug auggagcuca agaaagauac cgacaucaag     2340

gaaguccggc ccuacagccc caugcagcag gagauucuga gaaucuauga agagggcgcc     2400

cuucugcagc ucaagcgcga ugacgaggcu uuugccgaua uuucuaaaau cucccaaaug     2460

gcucagccua cagccacgga acugcggaga uacaagcugu ggcuugacca gaaauaucgc     2520

agcccguaca ccgggaaguu caucucccuc ucuaaacugu ucacaagcgc guaucaaauu     2580

gagcacguga ucccccaguc ccgguacuuu gacgauucuu ucagcaacaa gguuauuugu     2640

gaagccgagg ugaauagacu gaaauccaac augcucggau acgaauuuau caaggcucac     2700

ggcggggaga uuguccauug caccaugcug ggagacguga agauccuuag cgaaucugag     2760

uauaaagccu ucgugaauga gcacuacguu aaccaucgcg cuaagaaaga aaagcugcuc     2820

auggaggaua ucccucagga auuucugaac cggcagauga augacuccag auauauuagc     2880

aaagucguga aggcccugcu uucuaacauc gugcggacag aggaugagau ggacgucacc     2940

uccaaguucg ugguucccug cagcggcggg auuacggauc gccugaaaaa ggacuggggc     3000

cucaaugaug uguggaacuc uaucgucuac ccuagauuug aacggcugaa ucgcaugacc     3060

ggaacagagg ccuucggcca cugggaaaac aaagagggga agagaguguu ucaaaccagc     3120

guucccauug aacuccagcu gggauucucc aaaaagcgga ucgaccacag acaucacgcu     3180

auggaugcgc uugugauugc cugugcuagc cgcaauauca ucaacuaucu gaauaacgag     3240

ucugccaauu cccagaagaa acgggaggac cucagacgcc ugcugugcga caagaaccgg     3300

auuaucagaa aaccguggga aucuuuuaca caggaugccc gcgucgcccu ucaagacauu     3360

gugguuuccu ucaagaauua cgugcggauc auuaacaaag cuaccaauua uuacgagaga     3420

uacgaugaac agggcaagaa gacgacagcg gagcagaaau cuagcgaaau gugggcuauc     3480

cgcaagccua ugcauaaaga gaccgucuuu gggcacguga accugaagag aaaaacaacc     3540

gugaagcuca agaacgccau cgagaauauu cccaacaucu guaauaaaga acugaaggcc     3600

uauauuaacg agcucgucga aaaacauuuc aauaccaagc agaugcuggc ucacuucaaa     3660

uccaucaacu accgguggaa ucgccaaaac guugagaagg uggaagugug gcaguauucu     3720

gacgagaaag agagaauggc cgcgacacgg aagccucuug auacgagcuu ugacaagaaa     3780

cgcauugaaa ccaucacaga uaccggaauc cagaagauuc ugcucaauua ccuggaggaa     3840

aaaggcgggg accccaacau cgcuuucaca ccugagggca uugccgagau gaauaagaac     3900

aucucccugu uuaaugaugg aaaacuucac caacccauuc ugaaggucag aguggccaag     3960

accaugggcg cuaaauauca gguugggcag acgggaaaca aggccgcuaa auacgugggc     4020

gcccaggaag ggacaaaucu cuauuucgcg aucuacgaga acgaagaggg agaacggucu     4080

uacaacgccg ucccgcugaa ugagguggcu gagagacuca agcaaggcau gagcccuguu     4140

cccgaaaaga acgagaagga cgugccccug aaguuuuauc uuuccccuaa ugaucugguc     4200

uacgugccga ccgaagagga caaacugagc aacgaaugca agcucgauaa ugagcgcauu     4260

uauaaaaugg uuucugccac cgggaagaaa ugucauuucc ugcccuacuc cguggcuaac     4320

aucaucuaua auaaggucga guuugaaagc augaacaaga ugggcaaagc ccuuucugga     4380

gagaugauua aggccgugug cuggaaacug gaaguggacc ggcucggcac aauccugaga     4440

gucauuaagu ga                                                         4452


<210>  4559
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4559
augaagaccg agcuggaccu caacaaagau agacuguaca agcacgugcu uggccuggac       60

cucgggguca caagcaucgg auucgcccau guuguggaau ccgagaaucg gaacgaaucu      120

gagauuaaac agaucggcgu gcgcgucauu caacuggaua auuuugacaa gguggauaaa      180

agcgggaaga cguccgaauc uaagaacccc gcuguugacu ucgccgcggg aaaaggccuu      240

accccuaaug ggagcagaac aaagaaacgg ggagcucgca gaaaccugga ucgguaucag      300

gaccgcagag auaaucuccu ggaggugcug accaaguccg gcuuuaucaa cucugagaca      360

aaacggaccg aagacggcaa gcacacaacc caucagacgu gggagauucg cgccaaggcc      420

gucagcgaaa aaguggagaa ggaggaauuu gcuagaaucc uccuggccau caauaaaaag      480

cggggguaca aauccagccg caaggcuaaa aacgaggaug aaggacaggc cguugacggc      540

auggcggugg ccaagagacu uuaugaggaa aaucugaccc cggggcaaua cgcuuaugag      600

guccucucug gaggcaagaa auccauuccc gauuuuuacc ggagcgaccu gcaggccgag      660

uucgacaaga ucuggaacuu ucagaaacag uucuauccua auauucuuac agaugaauuu      720

uacgaggaac ugaaggggaa aggacaacgc gcuaccucug ccgcguucug gaagauccac      780

caguuuaaca cagccgacau uaaggaucuc gaggacgaac ugaaaaauga gagaaccauc      840

aagcugucca aacgggauca gaagaaacuc caggcuuaca aguggagaag cgaggccgug      900

gugaagcaac ugggcaaaga agaggucgcu uauguucuua cggaaauuaa caacaaucug      960

aacaauucuu ccggcuaccu cggggccauc agcgaccgcu ccaaggagcu guauuucaac     1020

aaacagacag ugggacaguu ucuuuacaag caacugaaag aaaaucccca uacccggcuc     1080

aagaaccagg uguucuauag acaggauuac cuggacgagu ucgagacaau cugggaaaug     1140

caggcccaau uucacccuga gcugacccag gaacucaagg aggagauucg cgauaucauu     1200

aucuucuauc agcggaaacu gaagucucag aaaggccuua uuagcuuuug cgaacuggag     1260

uccaaggaag ucgugaucaa ugacaccaaa aagaucauug ggcucagagu ugcucccaaa     1320

agcucuccuc uguuccaaga guuuaagauc uggcagaacc uuaauaacgu gcugguccgc     1380

aagaaaggau cccggaagag aauggugaaa gcgcagaauc agcacagccu cuucgaggaa     1440

ccgaaggaga uuuucucucu ggaucuggcc agcaaagaaa aacucuucga ggaacugaac     1500

cuuaagggca aucuguccgc uaaaaacugu cucgacaucc uggggcuuaa uucuaacgag     1560

ugggaacuga acuacacaca acucgaggga aaucgcacga acaagagccu guacgaggcc     1620

uuucugaaaa uucucgacau cgaaggcuau gauguuaagg accugcuuaa agugaagucc     1680

agcaaggaug aggucgaacu ggacgaucuc aaagugcccg ccucugagau caagaauaug     1740

guccgggaaa uuuucuccac caacaaaauc gacacagaga uucuggauuu ugacgcugag     1800

aucgauggca aggccuucga aaaacagagc ucuuaccagc uguggcaucu ucucuauucc     1860

uacgaggggg acgauagcca guccggaaau gaaaagcugu augagcugcu cgcgaagaaa     1920

uuuggcuuca agagagagca cgcucaaauu cuggccaacg ugucucuugu ugacgauuac     1980

gggaaucuga gcuccaaagc caugcgcaag aucuauccuc auauucggga aaacaauuac     2040

ucuaccgcuu gcgagcucgu gggauacaga cacagcaaac acucccugac aaaggacgaa     2100

cugaaagacc ggguccuuga ggaucgccuc gaacugcuga agaagaacag ccucagacag     2160

cccgugguug agaaaauccu gaaucagaug aucaacgccg ugaaugcucu uauugagaag     2220

aacucugaac gggacgagaa uggcaacauc accaaauauu ucaaguuuga ugaaauuaga     2280

aucgagcugg cccgcgaacu caaaaagaac gcggccgagc gggcugagau gacgacaaau     2340

auuaacgccg cuaagcuggu ccaugaaaag aucuucaaga uccugcaaaa ugaguuuggg     2400

auuaaaaacc cuaccagaaa ugacaucauu cgcuacagac uuuaugagga acucaagggc     2460

aacggauaca aagaucugua uuccaauacc uacauccccc gggaaauucu guucagcaac     2520

cagaucgaca ucgaucacau ucucccgcag ucucgccugu uugacgauuc cuucagcaau     2580

aagguggugg uuuuuagaaa ggacaaccuu aaaaagggca aucagacagc caucgauuau     2640

auugggucug aggucggaga ggaaggccug aaaaacuuca ucgagcgggu ggaaagccuc     2700

uacgagcugg ggaagaaaaa uaaggaggaa ggaauuucca aggcgaaaua ccaaaagcug     2760

cuuaaaagcg ccucugagau cggcgacggg uuuaucgaac gcgaucugag agagucccag     2820

uauauugcua agaaagccaa ggaacuccug uucaagguga cccggagcgu cguguccaca     2880

ucuggcagca ucacggaccg ccucagagag gacuggggac ugauuaacgu uaugaaagag     2940

cuuaaucugc cuaaguacaa aucccucggc cugaccgaaa uccaggagcg gcgcaacggg     3000

caucaggugg aagucauuaa ggauuggaca aaaagaaacg accaccggca ucacgcuaug     3060

gaugcccugg ccguggcuuu uaccaagcac aaucauaucc aauaucuuaa cuaccugaau     3120

gcgcgcaaaa acgagucuca caagcuccau ggaaauauua ucgccaucga aacacuggag     3180

accgagguua agauugacga ucucggcaac aaaaagagag uguucaaaga acccaucccu     3240

aauuuucggc aggucgcuaa ggagcaccug gaaaacgugc uuauuagcca caaagccaag     3300

aauaagguug ugacgaaaaa caagaauaaa aucuccggga aggagaaacu gcagauuacc     3360

cucacaccca gaggacagcu gcauaaggaa accgucuaug gcaaguaccg cuucuaucaa     3420

ucuaaagacg agaaggugag cgccaaauuc gaucuggaga caaucgaaaa gguggcuaac     3480

cccuccuaca aagccgcgcu ucugcagcgg cucaaugaga acgaaaauga cccuaagaag     3540

gcuuuuagcg ggaaaaacgc ccugucuaag aauccgaucu acauuuccau ggagaacgau     3600

aucaaagucc ccgacaccgu uaagcucgug uggcuggagg aagauuauag cauuagaaaa     3660

gacgugacgc cugagaacuu caaggacgaa aaaucuaucg agaagauucu ugaugaaggc     3720

gucaagcgca uccugaaaaa ucggcugaag gaguuuggag gcgacgccaa agaggcuuuc     3780

uccgaucucg acaagaaccc caucuggcug aaugaagaga aagggacaag cauuaagaga     3840

gugcgcaucu cuggaguuaa gaacgccgaa ccucuucacu acaaaaagga ucauuuuggc     3900

aaugacauuc uggaugggaa cggaaauaaa auccccgcgg acuucguguc caccggcaac     3960

aaucaccaug ucgcuauuua uaaggagccg gaauuugaua aaaaggggca gccugugcuc     4020

gacgagcugg gcaaccaaaa guacaaucuc caggagaaag uugugagcuu cuuugaagcc     4080

gucgagcggg ugaaccaggg acugcccauc auugauaaga cauauaauca gcaccuuggc     4140

uggcaauucu uuuucaccau gaaacagaac gaaauguucg uuuuuccuaa ugagaagacc     4200

agauucaacc ccaucgaaau cgaccugcug gauccuaaca auaaaaagcu cauuucccug     4260

aaccuuuuuc gggugcagaa gaucgccaau aaaaacuaca uguuccgcca ccaucuggag     4320

acaaaugucg acgacaagaa agagaugaag aacauugcuu uuauccacau ucagagcugu     4380

aaucuccuga aagaaaucau caaggugaga cucaaccauc ugggggauau ugugucuguc     4440

ggagaguauu ga                                                         4452


<210>  4560
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4560
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc caaagugucc       60

gucgaugacc acggcaauua ccuucaugau aucaagcugg ggucuagaau uauccccaug      120

agccaggaca uucucggaaa cuucgauaaa ggcguuacgg agucccaaac cgcuauccgg      180

acaggguuuc gcucugugag acggauccgc gaaagaagcc ugcuucggcg cgagagacug      240

caccgggugc uccaugucau gggauuccug ccuccgcacu augacuccgc cauuggcugg      300

aaucgcgaag aggcgucuac cuacggcaag uuucugaacc auggggaacc caaacucgcu      360

ugggagagac uggaggaugg aagcaugcgg uuccuuuuua uggacuccuu ccacgaaaug      420

auggccaauu uugcccgcca ccagccugcu cugguggccg auggcagaaa gaucccccuc      480

gacuggacac uguauuaccu ucggaagaaa gcucugagcc aggccguugg gaaggaggaa      540

cucgcgugga uucugcugaa cuucaaucag aaacgcggau auugccaacu cagaggcgag      600

gaggaagagg aaaccccuac aaagcgggag gaauacuaug agcugaaagu ggucucugug      660

gaggccgcug aagaggggaa gggaggcaac accugguaca gaguucagcu ugaaaauggc      720

uggaucuacc gccggcccuc caagauugcc cuggaugacu ggguggggaa aacgaagcag      780

uuuaucguca ccacagagua ugaaaaagau ggaaccacac ucaagaaaga caaggacggc      840

gaggugaaac gcagcuucag agcuccuggg gagaacgauu ggggacugcg gaagaagaga      900

accgaaucuc agcuugaggc cuccggcaaa acgguggggg cguuuauuua cgaccaucug      960

cucgccgaac cggcugauaa gauccgcgga aauuucaucc ggacaauuga gagaaaauau     1020

uacaagcaag aacuggaggc cauccugcgc gagcagacca aacaccauga ggaacuccgg     1080

agcaaggaaa ugcuggcuaa gugugccgag gagcuuuauc agucuaacca gccccacaga     1140

aaaucccugc ucaauaagga caugacauac cugcuucuga ccgaucucau uuucuaucaa     1200

cgcccucuga aaagcaagaa aucccugauc gccgaaugcc ccuacgagua cuauaaguac     1260

aaggacaaag aaaagggcaa aaagaaacgg cagggcauua agugugucgc uaagucuaac     1320

ccuuauuacc aggaguucag acucuggcaa uucaucguua aucugcggcu uuuugaucgc     1380

accgacgaua aagaagugac agcggaguau cugagaagcc gggaggacua cgccagacuc     1440

uucgcuuauc ugaacgaucg caaggaaauc aaccaggaga cgcugcuuaa agacuuuuuc     1500

gggcucaaga aagugaagau uggagccgaa aaagaguuug ccauccggug gaauuacauu     1560

gaagacaagg agaagaaaua ccccugcaac gagaccagac acgaacugcu gcucgcucug     1620

gaucgcgccg gcauggacag auccuggcuu gaugacagcg agcugcagua ucggcucugg     1680

caucugcugu acucugucga agcgcgcgau gaggcugaag gggcccuuag aaagcuccgg     1740

gacgaugacg aguucguggg agccuuucug aaaguuaagc cguucaaaaa ggauuauggc     1800

gcuuacuccg agaaggccgu gaaaaagcug cucgcuguca ugcgcauggg gagcuggugg     1860

cacgaagagg acaucuguaa acagacaaga gaucggauug aacgcaucgu gcaaggcgac     1920

aucgaggaga agauuagaga acggauggga caugccgcgu auucuuuuag ccgccuggcc     1980

gauuuccagg gccuuccuga guggcuggcu ugcuacguug uguaugggag acacuccgaa     2040

gccacccaua uccuccggug ggacucuccc gacaaacugc ugagcuacau uaagggauuu     2100

agacagcacu cccuucgcaa uccuaucguc gagcagugua uucucgaaac acugcggacc     2160

gugcacgaua ucuggaagga ggcuggccau auugacgaga uccacgucga acuggggaga     2220

agcaugaaau cuacggccga gcaacgcgcg cauaugacaa agaacauccu ccggaaugaa     2280

aacaccaauc ugagaauuaa augccuucug auggagcuca agaacgaugc cgacgugaaa     2340

gauguucgcc ccuacucccc caugcagcag gaaauccugc ggauuuauga ggagggagcu     2400

cugcagcaac uuaccaagga cgaugccgaa uacgcugaga ucagcaagau uucuaaaaug     2460

gcccagccuu ccgccaagga acuccugaga uauaaacugu ggcucgagca gaaguaccgc     2520

agcccguaua caggcaaacc caucucccug ucuaaacuuu ucaccagcgc uuaccagauc     2580

gaacacguga uuccucaauc ccgguauuuu gacgauucuu ucaauaacaa ggucaucugu     2640

gagagcgagg ugaauacacu gaaguccaac augcucgggu acgaauuuau uaagaaucac     2700

ugcggcgaga ucguucauug uacgaugcug ggagacguga aagucuuuag cgaaucugag     2760

uacaaggcgu ucgugaccga gcacuauucc aacaauaaau acaagaaaga aaagcugcuu     2820

cuggaggaua uuccccagga auuucucaac agacagauga acgacagccg guauaucucu     2880

aaggugguua aaggccugcu cuccaauguc gugcgcacag aggaugaacu ggaggccacc     2940

agcaaguucg uggucucuug cacaggggga aucaccgaca gacuuaaaaa ggacuggggc     3000

cugaacgaug uguggaauag cauuguuuac ucccgguuug agcgccucaa cgggcugacg     3060

aaaagcgaac auuucggaca cugggagaau aaggaaggca agagaguguu ucaaaccucu     3120

gucccuaugg agcugcagcg gggguucaac aaaaagagaa ucgaccaucg ccaccacgcu     3180

auggaugccc uugugauugc cugcgcuucc cggaauauca uuaacuaucu gaauaacgaa     3240

uuugccaaaa gccccaagca gagagaggac cuccgccagc ugcucuguga uaaaaaucgg     3300

aucaucagaa agccguggga gacauucacc caagacgcgc gcgcugcccu ggaagauguu     3360

guggucuccu uuaagaacua cgugcggguu auuaauaaaa caaccaacua uuacgagaga     3420

uacgacgccg auggcaagaa aacgauggug ccucagaagg gaaaugaaau gugggcuguc     3480

cgcaaaccca ugcaugaggg cacaauguau gggagaguga accuucggcg caaggccgug     3540

gucaagcuga aagaagcgcu cgagaacauc ucuagcauuu gcaauaagga gcugagagac     3600

uacaucaacg uucuggugga aaaacaguuc aauaccaagc agcuucuggc ucacuuuaaa     3660

uccauggauu aucgguggaa caagcaacau auugacaaag uggaggucug gcgcuucucu     3720

gacgaaaagg ugcagauggu ugccgccaga aagccucucg auaccagcuu cgacaaagag     3780

uguaucgcuu ccauuacaga uaccggaauc cagaagaucc ugcucaauua ccuggagucu     3840

aaaggcgggg accccgccau ugcuuuuaca agcgaaggaa ucgccgagau gaaccagaau     3900

auugcggugu auaacgaugg caagaaacac caaccuguca ucaaggugcg gaaguccacg     3960

aaacuuggga agaaauucgc cguuggccag auuggaugca agagcaaaca guuugugguc     4020

gcugaagccg gcaccaaucu guacuucgcu aucuaugagg acgaagaggg gaagagaucu     4080

uacgauacaa ucccccugaa cgaggugacc gaacgccuca agcaaggacu guccccgguu     4140

ccugagaaaa augaaaagga ggugccccuu aaauuuuacc ugagccccaa cgaccucguc     4200

uaugugccua cagaagauga gaugcagaug aaggagugua aacugaauaa ggaacggauu     4260

uacaagaugg ugucuuccac cggcaacgag ugccauuucc ucccguauag cgucgccucu     4320

guuaucuaca auaaagugga auuugagucc augaacaaga uggggagagc ccugacggga     4380

gaaaugauua aagcugugug uuggaaacuu gacgucgauc gccugggcaa cauccugcgg     4440

gugauuaagu ga                                                         4452


<210>  4561
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4561
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc caaaaucucc       60

auugaugaca auggcaacua ccuuagagau auccagcugg ggucucggau uauccccaug      120

agccaagacg ugcucggaaa uuucgauaac ggcgucacgg agucccagac cgcuguucgc      180

acaggguuua gaggacugcg gcgcaucaga gaacggucuc uucugcgcag agagcggcuc      240

caccgcgugc ugcaugugau gggcuuccug ccuccgcacu augacagcgc cauuggcugg      300

aauaaggaag aacccaccac auacgggaaa uuucucaacc auggagaacc uaagcuggcg      360

ugggagagac agggcgaugg guccaugcgg uuccuuuuua uggacucuuu ccacaaaaug      420

auggaggauu uugcucccca ucagccugcc cuggucgccg acggacgcaa gaucccccuc      480

gauuggaccc uguauuaccu uagaaagaaa gcucugacac aaccuauuaa gaaagaggag      540

cucgccugga uccugcugaa uuucaaccag aagcggggcu auuaccagcu ccgcggggaa      600

gaggaggaag agaauccgac caaaagagaa gaguaucacg aacugaaggu gguuggagug      660

gaggcugccg aggaaggcaa gggcgggaac acgugguaca augucaaacu ugagaacgga      720

uggauuuacc ggagaagcuc caagaucccc cuggacgauu gggugggcaa aacccgcccu      780

uuuauuguua caaccgaaua ugaggcggac gggaucacac ucaagaaaaa uaaggaagga      840

gaggugaaac ggagcuucag agcccccggc gaggacgauu gggggcugug gaagaagcgc      900

accgaaucuc agcuugaggc uuccggaaaa acggucggcg ccuuuaucua cgaccaccuu      960

cucgcugaac cugccgauaa gauucggggc aacuucauca gaacaauuga gcgcaaauau     1020

uacaagcaag aacuggcgga cauccugcgg gagcagagca aauaucauga ggaacuccag     1080

ucuaagaaga uucuggccga uugcgcugag gaacuuuaca gauccaauca gccccaccgc     1140

gacagccugc ucaaaaagga uaugguguau cugcuucuga ccgaccucau cuuuuaccaa     1200

cggccgcuga aaucuaagaa aucccugauc gccgauuguc cuuacgagua uuacgaguau     1260

guggacaagg aaacagggga gauuaagaga cagggaauca aaugcauugc uaagagcaac     1320

cccuacuauc aggaguuccg ccucuggcaa uucguccuga accuucggcu guacgauaga     1380

accgaagaca aagaaguuac cgccgaguau cucccuucca aggaggacua cgcccggcug     1440

uuuguguacc ugaaugaucg caaagaaauc aaccaggaga cacuucucaa ggacuucuuu     1500

aagcugaaaa aggugaaaca gagaucugaa aaggauuucc ccguccggug gaauuauauu     1560

gaggacaaag aaaagaaaua ccccuguaac gagacgagac augagcugcu cauggcucug     1620

gaucgcgcgg gcaucgaacg gagcuggcuu gacgauaccg ccagacagua ucgccugugg     1680

caccuccugu acuccaucga ggugaaggac gaagcugaga gagcccugcg gaagcugaaa     1740

gaugacgaug aauuuguuag cgccuuccuc aaggugaaac cuuuuaagaa agacuauggg     1800

gcuuacucug agaaggccgu caagaaacug cuggcgguga ugcgcauggg aucccacugg     1860

ugcgaggaug acauuuguaa ggaaacaaaa gagagaaucg aacauauucu ccaaggcgac     1920

guuggggaga agaucaaaga gcggaugaau cacaccgcuu auagccuggg cgaacuuucu     1980

gauuuccagg cccugccgga guggcucgcc ugcuacgugg ucuacggacg ccauagcgaa     2040

gcuacagaca uuaagcacug gaagaccccc gagucccuga uggccuauau caacggcuuu     2100

aaacagcauu cucugagaaa uccuauugug gaacagugua uccuugagac gcuccggaca     2160

guccacgaua ucuggaagga ggcugggcac auugacgaaa uccaugugga gcugggacgc     2220

agcaugaaau ccaccagcga ucaaagagcc cggaugaccg aaaacauucu gcgcaaugag     2280

aacacaaauc ucagaaucaa gucucugcuu auggaacuga aaaacgacgc ggauauuaag     2340

gacguucggc ccuacucccc uaugcagcag gagauccuca gaaucuauga ggaaggcgcc     2400

cugcaggagc ugaccaagga agauaaugac uacgaggaaa uuuucaaaau cagcaagaug     2460

ccccaaccgu cugcuaaaga gcuucucaag uauaaacugu ggcuggagca gaaguaccgc     2520

uccccuuaua cagggaaacc cauuagccuc uccaagcugu uuacgucugc cuaccagauc     2580

gaacacauua ucccucagag ccgguacuuc gaugacuccu uuaacaauaa ggugaucugc     2640

gagucugaag ucaacgcucu uaaaggaaac augcugggcu augaguucau uaagaaucau     2700

gggggcgaga ucgugcacug uacccuccug ggagauguua aagugcaaaa cgaaagcgag     2760

uacaaggccu ucgucacaga acacuaugcg aauaacaaau acaagaagga gaaacugcuu     2820

auggaagaca uuccccagga cuuucugaau agacaucuca acgauucccg cuauaucagc     2880

aaggugguga aaacccugcu cucuaauauu guucggacag aggacgagau ggaaaccacg     2940

uccaaguucg ucgugagcug caccggcggg aucacagaua gacugaaaaa ggacugggga     3000

cuuaacgaug uguggaacuc uaucgucuac ucccgcuuug agcggcugaa ugaacucacc     3060

aagagcgacg ccuucggcag augggagaau aaagaaggga agcgcguguu ucagacaucu     3120

guuccucugg agcugcaacg gggauucaac aaaaagagaa uugaucaccg gcaucacgcu     3180

auggacgccc uugugaucgc uugugccagc cgcaauauua ucaacuaucu gaauaacgag     3240

uccgccaaca gccccaaaaa gagagaagau cuccggcagc agcugugcga caagaaucgc     3300

auuaucagaa aaccguggga gucuuuuacc caggaugcuc gggcggcccu cgaagacguc     3360

gugguuuccu ucaagaacua cgugagaguc auuaauaaag cuacgaacua cuaugagcgc     3420

uacgacgccg auggcaagaa aacaaucacc aagcaaaaga gccgggaaau gugggccgug     3480

agaaaaccua ugcauaagga gacaguuuuu gggcacguga aucugcgccg gaaagcuauc     3540

gucaaacuua aggacgcccu ggagaacauu cccuccaucu gcaauaagga acucagagau     3600

uauauuaacg gccuggugga gcgcaauuuc aacaccaaac agcugcacgc gcauuuucgg     3660

ucuaccgacu acagauggaa uaagcagaac guggauaagg ucgaaguuug gcgcuucagc     3720

gacgagaaag agccccuugu ggcuacaaga aagccucugg auacguccuu ugacgccaaa     3780

cggaucgaua ccauuacaga caccggaauc cagaagaucc uccugaauua ucucgaagcc     3840

aaaggcgggg auccgcugau ugcuuucaca cccgagggaa ucgccgaaau gaaccaaaau     3900

auugcgcuuu acaacgacgg caagaugcac cagccuaucc ugaaggugcg cauuucugag     3960

cccaaagggg cuaaguauca gaucggacag accggcaaca aagccgauaa guucgucgaa     4020

gcccaaaaag ggacgaaucu guacuuugcu aucuacgagg acgaggacag aaaccggaca     4080

uauaauacca uuccucucaa cgaaguggcc gagcgccuga agcagggccu ucccccuguu     4140

cccgaaaaga augagaaaga agugccgcug aaguucuacc ucagcccuaa cgaucugguc     4200

uaugugccca ccgaggagga aauccucaug aaagagugua agcugaauaa aaacagaauu     4260

uacaagaugg uuuccucuac aggaaaucag ugcuuuuuca ucccuuuucg gguggcuguc     4320

agcauuguga acaagaugga auauagcccc cuuaauaaaa uggagaaggc caucuccucu     4380

gaaaugauua aagcgguuug uuggaagcug gagguggaca gacugggcaa caucauccgc     4440

gucgugaaau ga                                                         4452


<210>  4562
<211>  4458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4562
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugggc caaaaucucc       60

auugaugaca acggcaagua caucaaagau ucuauuaaac uugggagcag aaucaucccc      120

augucccagg acgugcuguc uaauuucagc aaaggaguca cacucuccca aacgucugag      180

cggaccagcu uucgcggcgc uagacggcug cgcgaaagaa cacuucugcg gcgcgagaga      240

cuccaccgga uucugcaucu gcucggguuc cugccuaagc acuaugaaaa ggccaucgga      300

ugggaucgca acgacaauaa aaccuacggc aaguuuauua accaugcgga gccgaaacuu      360

gcuuggugca aggauaauaa cggcaaaaug agauuccugu uuauggacuc cuuccacgau      420

augcucagcg aauuuucuga gaagcauccc gagcugcuug ccaaugggaa gaaaaucccu      480

cuggacugga caauuuauua ccuccggaag aaagcccugu auaaggcuau caccaaagaa      540

gaacuggccu ggauucuccu gaacuucaau cagaagcgcg gauacuauca gcuuagaggc      600

gaagaggauc gggacuccga ggcuaacaaa aaggaauacc uggagcucaa gguuaucggg      660

guggaagcca caggagagaa aaagggcaau gauaucuggu acaacauuca ccuggaaaau      720

ggguggaucu aucgcagaag cucuaaaaac ccccuuaacg acuggcuggg aaaggugaaa      780

uccuuuauug ucaccacgga ccucgaugcg gacggcaauc cuaagaagga uaaagacggc      840

aacaucaagc ggagcuucag agcccccucu gagggggauu ggacccugau uaaaaagcgc      900

acagaguccc ugcucgaaaa uagcggacag accgugggcc aauuuaucua caaccacauc      960

cuggaggaac cuuccgcuaa aauucggggg aauuucauca gaacaauuga gcgcaaguau     1020

uacaaggacg aacuuaaagc cauccuggag aagcagaaag aguuucauca cgaacucucu     1080

agcgaguccc ugcuuaccga augugcuaag gagcuguaua aacggaacau ugcccaucag     1140

gaaguucucc ugaagaaggg aaugauccac cuucuccuga augauauccu uuucuaccag     1200

agaccgcuga aaauuaagaa aagccucauc gacaacugcc ccuaugagaa uuacaaguac     1260

guggauaaaa agucuggcga cauuaaaguc aaguccauca aguguauugc gaaaagcaac     1320

ccuuucuuuc aagaguuccg ccuguggcag uuugugucua aucugcggcu uuauagaaag     1380

agcgaugaca aagacguuac guccgaacac cucgugacaa ccgaugacua cgccaaccug     1440

uucacauauc ugaauaaccg caaggauauc aaacagucug uccuccugaa ggaguacuuu     1500

aagauuaaaa aggugaaaac caucaauggg gcugaauauc ccgugcggug gaacuacauc     1560

gacgaugaca acaagaccua uccuugcaau gagacaagau acgauauucu ucaugcccug     1620

agcaaaacgg agcucaaguc cgaauggcug acccccgagc gcacauaccg gcuguggcac     1680

cuucucuaua gcaucgaauc uaaggaggaa accaaaaagg cucugaacaa acugaaucuc     1740

uccgacgauu ucacagaggc cuuucugacc auuaagccgu ucaaaaagga guacggaagc     1800

uauucugaaa aggccaucaa aaagcugcug ucccucauga gaaugggcua ccuguggagc     1860

gaggacgaag cuauuaaaca ggucggcgcg gagaucgaaa agacgaucga gggggagcug     1920

ccugauuaug uucuucaacg gcucgaaaaa ucccagcaua acuacaauaa gauuucugac     1980

uuuagcggac ugccccugug gcucgccucc uauaucauuu acggccgcca cucugaggcu     2040

aaagaaauca uucauuggga uaacccugag agcaugaugg ccuacaucaa ggacuucaga     2100

cagcacucca ugcggaaucc caucguggaa caaugugugc uggagacacu uagaaccguc     2160

cacgacauuu ggaaggagau cgggcauauu gaugaaaucc acauugagcu gggacgcgaa     2220

augaaaagca ccucugagca gcggaaggcc aucacacaga acgugcucca uaaucagaac     2280

accaauauca gaauuaaacu gcugcuucaa gagcucaaga acgacaauaa caucacaggc     2340

guucgccccu auuccccuau gcagcaggaa auucugagaa ucuacgagga aggggcucug     2400

cagggccuca cgaaagagga uaaggacuau aaugauauuc ugaagaucag ccaaaccgcc     2460

cagccgacau cuaacgaacu uacccgguac aaacuguggc ucgagcagaa guaucgcucc     2520

cccuacacag gaaaagcgau uagccugucu aagcuguuua ccagcgcuua ucagaucgag     2580

cacgugaucc cucaauccag auacuucgac gauagccuuu cuaacaaagu cauuugcgaa     2640

uccgagguga auacgcugaa gaaggccaug cucggcaugg aauuuaucaa aaccuguggg     2700

ggacagaagg uucacugcac acugcucggc gacguggaga uuuacaacga agaggauuau     2760

aaagccuucg ucaaugagca uuacagcaac aaucggacca agcgcaacaa acugcuuaug     2820

gaagacaucc cccaggaguu cauuaauaga cagcugaacg auucccggua uaucucuaag     2880

auggugaagc aacuccugag cgcuaucguc cgcgggcagg aagaggugga agccacaucc     2940

aaauuuguua uuccuuguac cggaggcauc acggacagac ugaagaaaga uugggggcuu     3000

aaugacauuu ggaacaaucu ggugucuccc cgguuccagc gcaugaacgc ucucacaggc     3060

agcgaguacu uuggacacug ggagaauaag gaaggcaaaa gagucuucca aaccacaaug     3120

ccgauccagc ugcagcaggg guuuuccaag aagcggauug accauagaca ccaugccaug     3180

gaugcgcucg ugaucgccug cgcuucucgc aacaucauua gcuaucugaa uaacgagacc     3240

gcccaagaca ccgaacggag acgcgaucuu cggcagaaac ugugugacaa gggaggcucc     3300

guuagaaaac cuugggauag cuucacacag gacacgcagu acgcucucaa ggauaucauu     3360

gugaccuuua aaaacuaugu ccgcaucauu aauaaggcca caaacuacua cgagcacuau     3420

aaaaccgacg ggaagaaggg aaugaucucu caaaaaggcg augagcugug ggcgauucgg     3480

aagcccaugc acaaagaaac aguguucggg aaggugaauc ugagacgccc uaaacggguu     3540

ucccuuagca aggccaucga cucugucgau gugaucugca acagaaagcu gaaaaaguac     3600

auuaaagcuc ggcucaauga gggccugcag aagaaacaga uccucaacau uuuuaaggaa     3660

gugaauaacg ucugggaggg aaucgacgug aauaagauug acaucuggga aauguccaac     3720

gauaaagagc ccaugguugc cacccgcaag cagcuggaca cgagcuucga ugagaaaaag     3780

aucagaucua uuuccgacac aggcauccaa aagauucuuc ugaauuaucu gaucaccaag     3840

aacaauaacc cugaacucgc uuuuaccccc gaggggauuc aggaaaugaa uagcaacauc     3900

uccgaguaca augaucauaa gaaacaccag ccgaucuaua aggugcgggu cacagaaccu     3960

cugggagcca aauaccccau uggcaccaug gggaacaacc ccaagaaauu cguggaggcc     4020

caagcuggaa caaaucuuua uuuugcgauc uacgaggaca aggaaggcaa ccgcaauuac     4080

gauacgauuc cucugaacau cgccgcugag agacucaauc agcagcugag cccgguuccc     4140

gaaaccaacg agaauaacac accucucaaa uucuaucugu cucccaauga ccuuguguac     4200

gucccuagcg aagaggagcg gcauuccggg aacaauauuc agcuggauaa gaacagaauc     4260

uauaagaugg ugucuagcac cuccaaacaa ugcuucuuua uucaccaucg cguugccauc     4320

ucucuggugg acaagaaaga guucagcgcc cagaauaaga uggagcgguc caucgauaac     4380

gaacagguca ugauuaaauc uguguguugg aagcucauca cagacagacu gggcaauauu     4440

aucaagguga uuaaauga                                                   4458


<210>  4563
<211>  4458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4563
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugggc caaaaucucc       60

auugaugaca acggcaagua caucaaagau ucuauuaaac uugggagcag aaucaucccc      120

augucccagg acgugcuguc uaauuucagc aaaggaguca cacucuccca aacgucugag      180

cggaccagcu uucgcggcgc uagacggcug cgcgaaagaa cacuucugcg gcgcgagaga      240

cuccaccgga uucugcaucu gcucggguuc cugccuaagc acuaugaaaa ggccaucgga      300

ugggaucgca acgacaauaa aaccuacggc aaguuuauua accaugcgga gccgaaacuu      360

gcuuggugca aggauaauaa cggcaaaaug agauuccugu uuauggacuc cuuccacgau      420

augcucagcg aauuuucuga gaagcauccc gagcugcuug ccaaugggaa gaaaaucccu      480

cuggacugga caauuuauua ccuccggaag aaagcccugu auaaggcuau caccaaagaa      540

gaacuggccu ggauucuccu gaacuucaau cagaagcgcg gauacuauca gcuuagaggc      600

gaagaggauc gggacuccga ggcuaacaaa aaggaauacc uggagcucaa gguuaucggg      660

guggaagcca caggagagaa aaagggcaau gauaucuggu acaacauuca ccuggaaaau      720

ggguggaucu aucgcagaag cucuaaaaac ccccuuaacg acuggcuggg aaaggugaaa      780

uccuuuauug ucaccacgga ccucgaugcg gacggcaauc cuaagaagga uaaagacggc      840

aacaucaagc ggagcuucag agcccccucu gagggggauu ggacccugau uaaaaagcgc      900

acagaguccc ugcucgaaaa uagcggacag accgugggcc aauuuaucua caaccacauc      960

cuggaggaac cuuccgcuaa aauucggggg aauuucauca gaacaauuga gcgcaaguau     1020

uacaaggacg aacuuaaagc cauccuggag aagcagaaag aguuucauca cgaacucucu     1080

agcgaguccc ugcuuaccga augugcuaag gagcuguaua aacggaacau ugcccaucag     1140

gaaguucucc ugaagaaggg aaucauccac cuucuccuga augauauucu uuucuaccag     1200

agaccgcuga aaaucaagaa aagccucauu gacaacugcc ccuaugagaa uuacaaguac     1260

guggauaaaa agucuggcga caucaaaguc aaguccauua aguguaucgc gaaaagcaac     1320

ccuuucuuuc aagaguuccg ccuguggcag uuugugucua aucugcggcu uuauagaaag     1380

agcgaugaca aagacguuac guccgaacac cucgugacaa ccgaugacua cgccaaccug     1440

uucacauauc ugaauaaccg caaggauauu aaacagucug uccuccugaa ggaguacuuu     1500

aagaucaaaa aggugaaaac caucaauggg gcugaauauc ccgugcggug gaacuacauu     1560

gacgaugaca acaagaccua uccuugcaau gagacaagau acgauauccu ucaugcccug     1620

agcaaaacgg agcucaaguc cgaauggcug acccccgagc gcacauaccg gcuguggcac     1680

cuucucuaua gcauugaauc uaaggaggaa accaaaaagg cucugaacaa acugaaucuc     1740

uccgacgauu ucacagaggc cuuucugacc aucaagccgu ucaaaaagga guacggaagc     1800

uauucugaaa aggccauuaa aaagcugcug ucccucauga gaaugggcua ccuguggagc     1860

gaggacgaag cuaucaaaca ggucggcgcg gagaucgaaa agacgauuga gggggagcug     1920

ccugauuaug uucuucaacg gcucgaaaaa ucccagcaua acuacaauaa gaucucugac     1980

uuuagcggac ugccccugug gcucgccucc uauauuaucu acggccgcca cucugaggcu     2040

aaagaaauua uccauuggga uaacccugag agcaugaugg ccuacaucaa ggacuucaga     2100

cagcacucca ugcggaaucc cauuguggaa caaugugugc uggagacacu uagaaccguc     2160

cacgacaucu ggaaggagau ugggcauauc gaugaaauuc acaucgagcu gggacgcgaa     2220

augaaaagca ccucugagca gcggaaggcc aucacacaga acgugcucca uaaucagaac     2280

accaauauua gaaucaaacu gcugcuucaa gagcucaaga acgacaauaa cauuacaggc     2340

guucgccccu auuccccuau gcagcaggaa auccugagaa uuuacgagga aggggcucug     2400

cagggccuca cgaaagagga uaaggacuau aaugauaucc ugaagauuag ccaaaccgcc     2460

cagccgacau cuaacgaacu uacccgguac aaacuguggc ucgagcagaa guaucgcucc     2520

cccuacacag gaaaagcgau cagccugucu aagcuguuua ccagcgcuua ucagaucgag     2580

cacgugauuc cucaauccag auacuucgac gauagccuuu cuaacaaagu caucugcgaa     2640

uccgagguga auacgcugaa gaaggccaug cucggcaugg aauuuauuaa aaccuguggg     2700

ggacagaagg uucacugcac acugcucggc gacguggaga ucuacaacga agaggauuau     2760

aaagccuucg ucaaugagca uuacagcaac aaucggacca agcgcaacaa acugcuuaug     2820

gaagacauuc cccaggaguu caucaauaga cagcugaacg auucccggua uaucucuaag     2880

auggugaagc aacuccugag cgcuauuguc cgcgggcagg aagaggugga agccacaucc     2940

aaauuuguua ucccuuguac cggaggcauu acggacagac ugaagaaaga uugggggcuu     3000

aaugacaucu ggaacaaucu ggugucuccc cgguuccagc gcaugaacgc ucucacaggc     3060

agcgaguacu uuggacacug ggagaauaag gaaggcaaaa gagucuucca aaccacaaug     3120

ccgauucagc ugcagcaggg guuuuccaag aagcggaucg accauagaca ccaugccaug     3180

gaugcgcucg ugaucgccug cgcuucucgc aacauuauca gcuaucugaa uaacgagacc     3240

gcccaagaca ccgaacggag acgcgaucuu cggcagaaac ugugugacaa gggaggcucc     3300

guuagaaaac cuugggauag cuucacacag gacacgcagu acgcucucaa ggauauuauc     3360

gugaccuuua aaaacuaugu ccgcauuauc aauaaggcca caaacuacua cgagcacuau     3420

aaaaccgacg ggaagaaggg aaugauuucu caaaaaggcg augagcugug ggcgauccgg     3480

aagcccaugc acaaagaaac aguguucggg aaggugaauc ugagacgccc uaaacggguu     3540

ucccuuagca aggccaucga cucugucgau auuaucugca acagaaagcu gaaaaaguac     3600

auuaaagcuc ggcucaauga gggccugcag aagaaacaga uccucaacau uuuuaaggaa     3660

cugaauaacg ugugggaggg aaucgacgug aauaagaucg acauuuggga aauguccaac     3720

gauaaagagc ccauggucgc cacccgcaag cagcuugaca cgagcuucga ugagaaaaag     3780

aucagaucua uuuccgacac aggcauccaa aagauucugc ugaauuaucu caucaccaag     3840

aacaauaacc cugaacuggc uuuuaccccc gaggggaucc aggaaaugaa uagcaacauu     3900

uccgaguaca augaucauaa gaaacaccag ccgaucuaua aggugcgggu uacagaaccu     3960

cuuggagcca aauaccccau uggcaccaug gggaacaacc ccaagaaauu cguggaggcc     4020

caagcuggaa caaaucugua uuuugcgauc uacgaggaca aggaaggcaa acgcaacuac     4080

gauacgauuc cucucaauau cgccgcugag agacugaacc agcagcucag cccggucccc     4140

gaaaccaaug agaacaauac accucugaag uucuaucuuu cucccaacga ccugguguac     4200

guuccuagcg aagaggagcg gcauuccggg aauaacauuc agcuggauaa gaauagaauc     4260

uauaaaaugg ugucuagcac cuccaagcgc ugcuucuuua uccaccaucg ggucgccauu     4320

ucucucgugg acaaaaagga guucagcgcc caaaacaaaa uggagagauc caucgauaau     4380

gaacagguua ugauuaaguc uguguguugg aagcugauca cagaccgccu uggcaacauu     4440

aucaaaguca ucaaguga                                                   4458


<210>  4564
<211>  4458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4564
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugggc caaaaucucc       60

auugaugaca acggcaagua caucaaagau ucuauuaaac uugggagcag aaucaucccc      120

augucccagg acgugcuguc uaauuucagc aaaggaguca cacucuccca aacgucugag      180

cggaccagcu uucgcggcgc uagacggcug cgcgaaagaa cacuucugcg gcgcgagaga      240

cuccaccgga uucugcaucu gcucggguuc cugccuaagc acuaugaaaa ggccaucgga      300

ugggaucgca acgacaauaa aaccuacggc aaguuuauua accaugcgga gccgaaacuu      360

gcuuggugca aggauaauaa cggcaaaaug agauuccugu uuauggacuc cuuccacgau      420

augcucagcg aauuuucuga gaagcauccc gagcugcuug ccaaugggaa gaaaaucccu      480

cuggacugga caauuuauua ccuccggaag aaagcccugu auaaggcuau caccaaagag      540

gagcuggccu ggauucuccu gaacuucaau cagaagcgcg gauacuauca gcuuagaggc      600

gaagaggauc gggacuccga ggcuaacaaa aaggaauacc uggagcucaa gguuaucggg      660

guggaagcca caggagagaa aaagggcaau gauaucuggu acaacauuca ccuggaaaau      720

ggguggaucu aucgcagaag cucuaaaaac ccccuuaacg acuggcuggg aaaggugaaa      780

uccuuuauug ucaccacgga ccucgaugcg gacggcaauc cuaagaagga uaaagacggc      840

aacaucaagc ggagcuucag agcccccucu gagggggauu ggacccugau uaaaaagcgc      900

acagaguccc ugcucgaaaa uagcggacag accgugggcc aauuuaucua caaccacauc      960

cuggaggaac cuuccgcuaa aauucggggg aauuucauca gaacaauuga gcgcaaguau     1020

uacaaggacg aacuuaaagc cauccuggag aagcagaaag aguuucauca cgaacucucu     1080

agcgaguccc ugcuuaccga augugcuaag gagcuguaua aacggaacau ugcccaucag     1140

gaaguucucc ugaagaaggg aaugauccac cuucuccuga augauauccu uuucuaccag     1200

agaccgcuga aaauuaagaa aagccucauc gacaacugcc ccuaugagaa uuacaaguac     1260

guggauaaaa agucuggcga cauuaaaguc aaguccauca aguguauugc gaaaagcaac     1320

ccuuucuuuc aagaguuccg ccuguggcag uuugugucua aucugcggcu uuauagaaag     1380

agcgaugaca aagacguuac guccgaacac cucgugacaa ccgaugacua cgccaaccug     1440

uucacauauc ugaauaaccg caaggauauc aaacagucug uccuccugaa ggaguacuuu     1500

aagauuaaaa aggugaaaac caucaauggg gcugaauauc ccgugcggug gaacuacauc     1560

gacgaugaca acaagaccua uccuugcaau gagacaagau acgauauucu ucaugcccug     1620

agcaaaacgg agcucaaguc cgaauggcug acccccgagc gcacauaccg gcuguggcac     1680

cuucucuaua gcaucgaauc uaaggaggaa accaaaaagg cucugaacaa acugaaucuc     1740

uccgacgauu ucacagaggc cuuucugacc auuaagccgu ucaaaaagga guacggaagc     1800

uauucugaaa aggccaucaa aaagcugcug ucccucauga gaaugggcua ccuguggagc     1860

gaggacgaag cuauuaaaca ggucggcgcg gagaucgaaa agacgaucga gggggagcug     1920

ccugauuaug uucuucaacg gcucgaaaaa ucccagcaua acuacaauaa gauuucugac     1980

uuuagcggac ugccccugug gcucgccucc uauaucauuu acggccgcca cucugaggcu     2040

aaagaaauca uucauuggga uaacccugag agcaugaugg ccuacaucaa ggacuucaga     2100

cagcacucca ugcggaaucc caucguggaa caaugugugc uggagacacu uagaaccguc     2160

cacgacauuu ggaaggagau cgggcauauu gaugaaaucc acauugagcu gggacgcgaa     2220

augaaaagca ccucugagca gcggaaggcc aucacacaga acgugcucca uaaucagaac     2280

accaauauca gaauuaaacu gcugcuucaa gagcucaaga acgacaauaa caucacaggc     2340

guucgccccu auuccccuau gcagcaggaa auucugagaa ucuacgagga aggggcucug     2400

cagggccuca cgaaagagga uaaggacuau aaugauauuc ugaagaucag ccaaaccgcc     2460

cagccgacau cuaacgaacu uacccgguac aaacuguggc ucgagcagaa guaucgcucc     2520

cccuacacag gaaaagcgau uagccugucu aagcuguuua ccagcgcuua ucagaucgag     2580

cacgugaucc cucaauccag auacuucgac gauagccuuu cuaacaaagu cauuugcgaa     2640

uccgagguga auacgcugaa gaaggccaug cucggcaugg aauuuaucaa aaccuguggg     2700

ggacagaagg uucacugcac acugcucggc gacguggaga uuuacaacga agaggauuau     2760

aaagccuucg ucaaugagca uuacagcaac aaucggacca agcgcaacaa acugcuuaug     2820

gaagacaucc cccaggaguu cauuaauaga cagcugaacg auucccggua uaucucuaag     2880

auggugaagc aacuccugag cgcuaucguc cgcgggcagg aagaggugga agccacaucc     2940

aaauuuguua uuccuuguac cggaggcauc acggacagac ugaagaaaga uugggggcuu     3000

aaugacauuu ggaacaaucu ggugucuccc cgguuccagc gcaugaacgc ucucacaggc     3060

agcgaguacu uuggacacug ggagaauaag gaaggcaaaa gagucuucca aaccacaaug     3120

ccgauccagc ugcagcaggg guuuuccaag aagcggauug accauagaca ccaugccaug     3180

gaugcgcucg ugaucgccug cgcuucucgc aacaucauua gcuaucugaa uaacgagacc     3240

gcccaagaca ccgaacggag acgcgaucuu cggcagaaac ugugugacaa gggaggcucc     3300

guuagaaaac cuugggauag cuucacacag gacacgcagu acgcucucaa ggauaucauu     3360

gugaccuuua aaaacuaugu ccgcaucauu aauaaggcca caaacuacua cgagcacuau     3420

aaaaccgacg ggaagaaggg aaugaucucu caaaaaggcg augagcugug ggcgauucgg     3480

aagcccaugc acaaagaaac aguguucggg aaggugaauc ugagacgccc uaaacggguu     3540

ucccuuagca aggccaucga cucugucgau gugaucugca acagaaagcu gaaaaaguac     3600

auuaaagcuc ggcucaauga gggccugcag aagaaacaga uccucaacau uuuuaaggaa     3660

cugaauaacg ugugggaggg aaucgacguc aauaagauug acaucuggga aauguccaac     3720

gauaaagagc ccaugguggc cacccgcaag cagcuugaca cgagcuucga ugagaaaaag     3780

aucagaucua uuuccgacac aggcauccaa aagauucugc ugaauuaucu caucaccaag     3840

aacaauaacc cugaacuggc uuuuaccccc gaggggauuc aggaaaugaa uagcaacauc     3900

uccgaguaca augaucauaa ggaacaccag ccgaucuaua aaguucgggu gacagagccu     3960

cuuggagcca aguaccccau uggcaccaug gggaacaaac ccaagaaauu cgucgaggcc     4020

caagcuggaa caaaccugua uuuugcgauc uacgaagaca aggagggcaa ucgcaacuac     4080

gauacgauuc cucucaauau cgccgcugaa agacugaacc agcagcucag cccggugccc     4140

gagaccaaug aaaacaauac accucugaaa uucuaucuuu cucccaacga ccugguuuac     4200

gugccuagcg aggaggaacg gcauuccggg aauaacauuc agcuggauaa gaauagaauc     4260

uauaagaugg ucucuagcac cuccaaacaa ugcuucuuua uucaccaucg cguggccauc     4320

ucucucguug acaagaaaga guucagcgcc cagaacaaga uggaacgguc caucgauaau     4380

gaacagguga ugauuaaauc ugucuguugg aagcugauca cagacagacu uggcaacauu     4440

aucaagguga uuaaauga                                                   4458


<210>  4565
<211>  4461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4565
augaccaaga uccugggccu cgaccugggg acaaacagca uuggaugggc caucagagag       60

gaugaaaaug agggcgugaa ccagauuauc gacaaagggg uccggauuuu cuccgaagga      120

guuaagucug agaauggcaa agaaaucagc cgcgcugccg agagaacggc guaccggucc      180

gcucgcaaga ucaaauauag acggaaacuu cgcaaguacg agacccugaa agugcucucu      240

gugaacggga ugugcccccu gagcgucgaa gagguggaac aauggaagaa auccggauuu      300

aaggaguauc cucuuaaucc ggaguuccug aacuggcuca gaacaaauga agauaaaaac      360

auuaaucccu acuuuuuccg ggacaaggcc ucuaagcaga aaguuacccu guuugagcug      420

ggccgcgccc ucuaucacau cgcucagaga cggggcuucc ugagcaaccg ccuugaucag      480

uccgccgaag ggguguuuga ggaacauaau ccucaaauuc agaaccugau cgaggaccuc      540

gauagcucua auacaauucu gaacgagcuu aaggaauacu auaucaaucu gggaauuauc      600

gacgaguccg aaaagagcgg cuucaagaaa gaucucgacg agggggaaaa gaaccugaaa      660

aagcuguaca ccucucucgu cuccaucaca aagaaaaacg cuaaugauau ugagaccugu      720

aagcaggagc ugaucgccag acuuaacaaa aaggaagacc ugggaaaagu gaagggcgag      780

auuaaggaua ucagccaggc gaugcucgac gggaauuuua aaacgcuggg acaauacuuc      840

uuuucucuuu auaacaagac ccggauucgc aaucaguaca cauccagaga ggagcacuau      900

cuggaagagu ucaucaucau uugccagacc cagggcaucg aggaaauuaa cacaaaugag      960

aaacuccccg aaaagaaauu uaccggccug gccaaggacc uguaccgggc uaucuucuuc     1020

caaagaccuc ucaagagcca gaaagggcug auuggaaagu guuccuuuga gaaaaacaag     1080

ucucgcugcg ccguuagcca ucccgauuau gaggaguucc ggaugugguc cuaccuuaau     1140

acgaucaaaa ucggcacaca gagcgaaaag acccugagau uucucacacu ggaagagaaa     1200

cuuaagcugg ugccuaaguu cuaucgcaaa aacgacuuua auuucgaagu ccucgcuaag     1260

gagcuggugg agaaaggggc cucuuuugga uacuacaagu ccagcaaaaa gaacgaguuc     1320

uuuuauuggu ucaauuacaa gccgaccgau ucugugagcg cgugugucgu uuccgccucu     1380

cuggaaaacg cuauuggcaa agacuggaag accaaaacau uugaauauca aacgagcaau     1440

accaagaaag aggaagugac aaaguccgug aacuacaagg aucucuggca ucugcuuagc     1500

gucgccaccu cugacacaua ucuguacgau uucgcuaucg agaaacucaa gcuggagccc     1560

aaaaacgcca aggccuuuuc caaaaccaag cugaagaaag acuucgcuag ccuuucucuc     1620

gcggccauug cuaagauccu gccuuaucug aaacaggggc uccuguacuc ccacgccgug     1680

uucauugcca auaucgaaaa cauuguugau gcugacaucu ggaaggauaa agagcagcag     1740

aaguuuaucc aaagcaagau uguggaccuu aucgacaauu accuggucga aaaauccaag     1800

cucgagauua ucaacugccu gcugaaaauu uauaauacgg aagauaucga gggacggaag     1860

guguacuauu cuaaagaggc cgaaagccuu uucgaggcgg accucagaaa gaaacugguu     1920

cccuuuuaca aggcuaacac aaucuccgaa cagcaggagc aggagauuau cuucaaggau     1980

cuguuuccug ugcucauuga acaacugaaa aagcaggagu ucaucaaaau uguccgccuu     2040

gacaagaaaa ucgaagccuu ucugaagggc gagaaugaag agggccagau cuucuguaac     2100

uaucccgaga aguuuaaaaa gcucuaccac ccgucugaua uugaaguguu caaaaagaaa     2160

aucauuaagg acgagugggg gaaugaaaag gucgugcugg gaagcccucu gaccaccucc     2220

aucaaaaacc ccauggccau gcgggcccuu caccagcuca gaaagguucu gaauacacug     2280

auucucgaug gccaaaucga cgagaaaacc cgcauccaua uugaaauggc ucgggagcug     2340

aacgaugcua auaagagaaa agggauccag gacuuucaga aggagcgcga ggaacuuuau     2400

aagauuuacg aagccaaaau caaggagcug uaccagguga aaacaggaaa ggaacuccug     2460

cacgucacgg augaggaccu gcaaaaauuc gagugggcgc uugaacagac caaggauggc     2520

cggauuauca caaaggagga acucauuaaa uauaagaucu gggaggagca gaaccauauc     2580

ugcauuuaca ccgggaaaac aaucagcauu gacucuuuuc ugggcggaaa uccuaaguuc     2640

gacaucgaac acaccauucc cagauccauc agccaagaua acucucugau gaacaaaacg     2700

cucugugaca aggauuucaa ucgguccgug aaaggcaaga agaugcccgu ugagcugagc     2760

aacuauaaug aaauccuucc ucgcauugcc cacuggaaaa agcaggcuga ggaacugacc     2820

agacagaucg agacaauuuc ucggagcauc aaauccgccg cuaccaaggu ggccaaagac     2880

aagaacauua gaaagcgcca uuaccucaca cugaaacggg auuauaucca ggggaaguac     2940

gacagauuua ccuggggaga gccgaaaguc ggcuucaaga auagccaaau ccccgauacg     3000

gggauuauca caaaauaugc gcaggccuac cugaagucuu acuuuaagcg cguggaaucc     3060

gugaaaggag gcgcuguugc cgaguucaga aaacuuuggg ggauucagga gagcuuuauc     3120

gacgaaaacg gcuucaagca cuuuaaggaa aaagaucggu ccaagcauac ccaccauaca     3180

auugacgcua ucaccauugc cugcaugccu aaggauaaau augaccugcu cgcccacgcu     3240

uggcgccugg aggaugagca gaauaagaaa gcggccaagg cucucaucga acaagccaaa     3300

cccuggaaga ccuucaaaga ggacaucgaa aagauugagg ccgaaauccu ggucucucac     3360

uuuacaccug acaacgugaa gaaacaggcu aagaaaauuc uuagagugcg gggaaagaaa     3420

caguacgucg ccgagaucga gcgcaaugcg aacggcaagg cuauucccaa gaaagaugcc     3480

agcgggaaga ucaucuauaa acugaaugaa agaggaaaca agauuccgau ccuccagcaa     3540

ggcgacacga uucggggguc ccugcaucag gauucuaucu acggagccau uaaaaauccu     3600

cugaacacca augagauccg cuaugugauc agaaaggacc uugaaagccu gaaggcuaac     3660

gauauugaga acaucguuga cgagaaugug aaagccaagg ucaaagaagc gguggcuaac     3720

aagauucucc ugaucuccuc uaauccccag cagaaaaaca aguucgccga gggcaagaau     3780

guuuggauga acgaagagaa aaagauuccu aucaauaaag ugcggauuua cgccaacagc     3840

gucaagaauc ccaucgaaau caaaaagcac uccacacuca gcaaaucuaa gcaugagcac     3900

aagcagaccg uguaugggca gaacgaugag aauuacgcua uggcccugua cgaacuugac     3960

ggcaaacgcg aguuugaacu gauuaacaau uucaaccucg cuaagcugcu gaaacaggga     4020

caaggcuauu acccucuuca caaggagaaa gaaaucaagg ggaagaaagu ucugauuccc     4080

aucgagaaga gaaauaaccg ggaugugguc cucaaaagag gacaacaggu gguguucuau     4140

gacaagacag ucgaaaauuc caaagauauu agcgaaaucg uugacuuucg cggccgggug     4200

uaccugaucg aggggcucac cauucagaga caaaaggaca agaaaacggg aaaggugaac     4260

gaauauggca ucauucagcu gcgcuacuuc aaagaggccc ggaagucuga agagaucucc     4320

aaagauagcu uuaagccgga cggggaguuc aagauuaacg aaaauaaacc uacaagaaag     4380

augaaccaua aucaguuuac cgcguucguc gagggcaucg auuuuaaagu gcuucccucu     4440

ggaaaguucc agaaaaucug a                                               4461


<210>  4566
<211>  4464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4566
augaagaacg ugcugggccu cgaccugggg agcaauuccg ucggaugggc ccuuguuaaa       60

accgaggaua acggcaaucc caucgggucu auuaagaugg gaagcagaau cauuccuaug      120

ucccaggaca uccugggcuc uuucgaaaaa ggggauacag ugagccaaac ggcucagcgg      180

acccucuacc gcggaaagag acggcugauu gagcgccacg ugcuuagacg ggaacgccug      240

ugcagagucc uccauaucau gaacuuucug ccggaccacu ucgauaaacu gcucggcugg      300

gacaagacag auaauaagac cuauggcaaa uuuaucgacg auuccgagcc caagcuggcc      360

uggcggcaga acaaaaauga aaaggagaaa auggaguucg uguuuaugga cucuuuccau      420

gaaaugcuua gcgauuuugc gaagcaccag ccucaacuga uugcuaacgg gaagaaaguu      480

ccccucgacu ggacaaucua cuaucugcgc aagaaagccc uuacccagcu gauuuccaag      540

gaggaacucg ccuggauccu gcugaauuuc aacaaaaaga gaggauacua ucagcuccgg      600

ggcgaggagg aagaggaaca gccuaccaaa aaggaggaau acaaggugcu gaaagucauu      660

agcguggaug cugacgaggg gcaaaaggga aauggcaucu gguauaacau ucaucuugag      720

gacgggggaa ucuacaaacg caagucugau acaccccugu acgacugggu uggcaaaacg      780

cuccagcuga ucguggucac cauuuaugaa aaggauggca agacaccuaa aaucuuugac      840

uccgggaagc agaauccgag cuacagacuu cccaaagagg augacugggg acugauuaag      900

aaacggaccg aaucucagau ccaauccagc ggcaagacag ugggggccuu cauuuaugau      960

aacauccucu cuaagccuga cgauaaaauc cgcggaaauc uggugagaac cauugagcgg     1020

aaguacuaua aaaaggaacu gaucgagauu cuccagcagc aagcuaaaua ccacgacgag     1080

cugcacaacc agaaccuucu ggaauccugu gccaaggagc ucuaugcgca uaaugauguc     1140

agauacaacg aaguugucaa gagcaauaug auccaccuuc uuguggacga cauuaucuuu     1200

uaccagcgcc cccugaaauc caagaaaucu cucaucgcca acugcaagua ugagagcuac     1260

gaauaugugg auaaagagac gggcgaggug aaggaaaugc cuauuaaaug uaucgcuaag     1320

uccaaucccu acuaucagga guuccggcug uggcaauuua uucauaaccu gagacucagc     1380

gacguuguga cacgcgaaga ugucaccgcc caguaccugu cuacaccgca ggacuauugc     1440

aaucuuuuca ccuaccugaa cgaucggaag gagaucaaac aggacauucu ccugaaggau     1500

uucuuuaaaa ucaagaaagu gcaaauugac aaggagaagg uuuucccuau cgaauggaau     1560

uacaucaaag augcuaccaa gacauauccc uguaacgaga cgagauacga acugcuuucc     1620

gcccucaaac gcgcggggau ggacaagcag uggcuggaug acaccccuaa uaugcaguau     1680

cggcuguggc accuccugua cagcguggag gaacaggagg agucugccaa agcucuuaga     1740

aagcuguaug auaacgacaa uuuugucagc uccuuccuca agauuaaacc cuuuaagaaa     1800

ugcuacggag ccuauucuga aaaggcuauc aaacgccugc ugacacuuau gcggaugggc     1860

aaggccuggg acgagcaagc cauugaugcu aagacccuca aauguaucca gcagauuauc     1920

acaggcaaca ucgacagcaa gcugaaagaa aagauugaug acuccagaua cgugcugcac     1980

caugucagcg auuuccaggg gcuccccacc uaccuggcgu cuuaugugau cuacggacgc     2040

cacuccgagg uuacggacau ucggugcugg gauacaccua aagaccuucu gaaguuuauc     2100

uguggcuucc cgcaacauag ccucagaaau cccauugugg aacaggucau ccuggagacc     2160

cugcgggugg uuuaugauau cuggaaagcc gagaagcaca uugacgaaau ccauguggag     2220

gucgcucgcg aaaugaagcu gaccgcccag caaagaaacg aggccaaccu ccggaaucug     2280

aacaaugaag cuacaaaccu gagaauuaaa uaccuccugg aggagcuuaa gaaugauacc     2340

uuuaucaaag acgugcgccc ucugucuccc augcaccagg aaaagaugcg gauuuaugag     2400

caggccgugc ucgggaaccu gaauaaacag gauaaggacu acgacgauau caucaagauu     2460

uccaaaaagg aaagaccuac aagcgcggag cugauccgcu auaaacuuug gcucgagcaa     2520

aaguacugcu cccccuacac gggaaaagcu auuucucuga gcaagcuguu caccacagcc     2580

uaucagaucg aacacguuau uccgcagucc agauacuuug acaacucuuu caauaacaag     2640

gucaucugug agagcgaagu gaauaaagag aaggauaaaa ugcucggcua ugaauuuauu     2700

aaggggcggg gcggagaccg caucuacugc accgcccaug gcaaagaggu gacaauccug     2760

aaggaggaag aguauaagca guucgucaac gaacacuacg cucauaauaa aagaaagcgg     2820

gauaaccuuc uggccgagaa uauuccugaa uccuucagcu cucgccaacu caacgacacc     2880

agauauauca cgaaaaccau uaugucccug cugagcaacg ugguucggca gcagggggag     2940

gaggaagcua cagccaagaa ucuucugccc ucuuccggag cggugaccga uaaacucaag     3000

aacgacuggg gccugaagga ugucuggaau acaaucguga ccccucgcuu ugagagacuc     3060

aacaaacuga gcgggacgaa ugacuucgga gaaaugaagg aggaaaacgg caaucgguac     3120

uuucagacaa acguucccuc ugaguacgag aaaggguuca agaaaaagcg cauugaucac     3180

agacaccaug ccauggacgc ucuugugauc gccugcacca cacggaauau ggucaacuau     3240

aucagcaaug ugaacgcuaa uuccccuaaa caaagagaag accugcgcca gcuccugugu     3300

gauaagaacc ggauuaucaa uaagcccugg aacaccuuua cccaggacgc ccugaaagcg     3360

cuuaacgaua uuguugugag cuucaagaau aacgucagaa ucauuaaucg cgccacaaac     3420

cgguaccaaa gauaugacaa aaauggcaag aaaaugaucu gcucucagaa gggaaacgau     3480

augugggcua uccgcaaguc caugcacaaa gagacggugu uuggccgggu gaaucugauu     3540

agaaaggaag cccucccgau cgcuaaagcc cuggacaaca uuagcgccau cuguaaugcu     3600

cagcuccgcg cguacgucaa cgaucugguu gacaagcauu ucaauaaaaa gcagcuugug     3660

gcccacuuua agucccugga uuauaaaugg aaccggcaag acgugucuaa ggucgaggug     3720

uggauuagcu ccgaugacaa aaccccuaug guugcuguga gaaagccccu cgauacaucu     3780

uucgacaaaa agaagaucgc cagcauuacc gacacaggga uccaggccau ccugcugaau     3840

uaccuugagu ccaaaggagg cgaugcucug gucgccuuug cgcccgaagg gauucaggag     3900

augaacucua auaucgaacu cuauaacaau ggaaaggcuc auaaaccuau uaagaacgug     3960

cggcugagcg agacccucgg cgccaaauac cagguugggg aacgcggcgc caagacgaaa     4020

aaguacgugg ucgcugacaa gggaacaaac cuguucuuug ccaucuauga ggaugaggaa     4080

ggcaaaagau acuauuacuc cgugccgcuu cgggaggcga uugaacgcca aaagcagaaa     4140

cugagccccg uuccugagua uaaugaaaag gggguggcuc ugaaauucua ccucucuccc     4200

aacgaccugg ucuaugugcc uaccgaggag gaaagaacca ugggaacaug ccacaucgau     4260

aagacccgga ucuacaagau gguguccagc ucugagaaua gauguugcuu cguccccucc     4320

acaauugcca aaguuaucgc cgacaaggug gaacuucuga gccacaacaa aauugagaag     4380

gcuaucacgg gcgaaucuau uaaagaggug uguuggaagc ucaaggucga ucgccugggg     4440

aauauccuca acgugaucaa auga                                            4464


<210>  4567
<211>  4464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4567
augaagaacg ugcugggccu cgaccugggg agcaauuccg ucggaugggc ccuuguuaaa       60

accgaggaua acggcaaucc caucgggucu auuaagaugg gaagcagaau cauuccuaug      120

ucccaggaca uccugggcuc uuucgaaaaa ggggauacag ugagccaaac ggcucagcgg      180

acccucuacc gcggaaagag acggcugaca gagcgccacg ugcuuagacg ggaacgccug      240

ugcagagucc uccauauuau gaacuuucug ccggaccacu ucgauaaacu gcucggcugg      300

gacaagaccg auaauaagac auauggcaaa uuuaucgacg auuccgagcc caagcuggcc      360

uggcggcaga acaaaaauga aaaggagaaa auggaguucg uguuuaugga cucuuuccau      420

gaaaugcuua gcgauuuugc gaagcaccag ccucaacuga ucgcuaacgg gaagaaaguu      480

ccccucgacu ggaccauuua cuaucugcgc aagaaagccc uuacacagcu gaucuccaag      540

gaggaacucg ccuggauucu gcugaauuuc aacaaaaaga gaggauacua ucagcuccgg      600

ggcgaggagg aagaggaaca gccuaccaaa aaggaggaau acaaggugcu gaaagucauc      660

agcguggaug cugacgaggg gcaaaaggga aauggcauuu gguauaacau ccaucuugag      720

gacgggggaa uuuacaaacg caagucugau aucccccugu acgacugggu uggcaaaacg      780

cuccagcuga ucguggucac cauuuaugaa aaggauggca agacaccuaa aaucuuugac      840

uccgggaagc agaauccgag cuacagacuu cccaaagagg augacugggg acugauuaag      900

aaacggaccg aaucucagau ccaauccagc ggcaagacag ugggggccuu cauuuaugau      960

aacauccucu cuaagccugc ugacaaaauc cgcggaaauc uggugagaac cauugagcgg     1020

aaguacuaua aaaaggaacu gaucgagauu cuccagcagc aagccaaaua ccacgaugag     1080

cugcacaacc agcaucuucu ggaauccugu gcgaaggagc ucuaugccca caacgacguc     1140

agauacaaug aaguugucaa gagcaacaug auccaucugc uuguggauga cauuaucuuu     1200

uaccagcgcc cccugaaauc caagaaaucu cucaucgaca auugcaagua ugagagcuac     1260

gaauaugugg auaaagagac gggcgaggug aaggaaaugc cuauuaaaug uaucgcuaag     1320

uccaaccccu acuaucagga guuccggcug uggcaauuua uucacaaucu gagacucagc     1380

gacguuguga cacgcgaaga ugucaccgcc caguaccugu cuacaccgca ggacuauugc     1440

aaccuuuuca ccuaccugaa ugaucggaag gagaucaaac aggacauucu ccugaaggau     1500

uucuuucaaa ucaaaaaggu gcagauugac aaagagaagg uuuucccuau cgaauggaac     1560

uacaucaagg augcuaccaa aacauauccc uguaaugaga cgaaguacga acugcuuucc     1620

gcccucaaaa gagcggggau ggacaagcag uggcuggaug acaccccuaa caugcaguau     1680

cgccuguggc accuccugua cagcguggag gaaaaagagg agucugccaa ggcccuucgg     1740

aagcuguaug augacgacaa uuuugucagc uccuuccuca aaauuaagcc cuuugaaaaa     1800

ugcuacggag cuuauucuga gaaggcuauc aaaagacugc ugacacuuau gcgcaugggc     1860

aaggccuggg augaacaagc cauugacgcu aagacccuca aauguaucca gcagauuauc     1920

acaggcaaca ucgauagcaa gcugaaagag aagauugacg auucccggua cgugcugcau     1980

cacgucagcg acuuccaggg gcuccccacc uaccuggcgu cuuaugugau cuacggaaga     2040

cauuccgaag uuacggauau ucgcugcugg gacacaccua aagaucuucu gaaguuuauc     2100

uguggcuucc cgcaacacag ccuccggaau cccauugugg agcaggucau ccuggagacc     2160

cugagagugg uuuaugacau cuggaaagcc gaaaagcaua uugaugagau ccacguggaa     2220

gucgcucggg agaugaaacu uaccgcccag caacgcaacg aagccaaucu cagaaaccug     2280

aacaaugagg cuacaaaccu gcggauuaag uaccuccugg aggaacuuaa gaaugacacc     2340

uuuaucaaag augugagacc ucugucuccc augcaccagg agaagaugcg cauuuaugaa     2400

caggccgugc ucgggaaccu gaauaaacag gacaaggacu acgaugacau caucaagauu     2460

uccaaaaagg agcggccuac aagcgcggag cugaucagau auaaacuuug gcucgaacaa     2520

aaguacugcu cccccuacac gggaaaagcu auuucucuga gcaagcuguu caccacagcc     2580

uaucagaucg agcauguuau uccgcagucc cgcuacuuug auaacucuuu caauaacaag     2640

gucaucugug aaagcgaggu gaauaaagaa aaggacaaaa ugcucggcua ugaguuuauu     2700

aaggggagag gcggagaucg gaucuacugc accgcccacg gcaaagaggu gacaauccug     2760

aaggaagagg aauauaagca guucgucaac gagcauuacg cucacaauaa acgcaagaga     2820

gacaaccuuc uggccgaaaa uauuccugag uccuucagcu cucggcaacu caacgauacc     2880

cgcuauauca cgaaaaccau uaugucccug cugagcaaug ugguuagaca gcagggggag     2940

gaagaggcua cagccaagaa ccuucugccc ucuuccggag cggugaccga caaacucaag     3000

aacgauuggg gccugaagga cgucuggaau acaaucguga ccccucgguu ugaacgccuc     3060

aacaaacuga gcgggacgaa ugauuucgga gagaugaagg aagagaacgg caauagauac     3120

uuucagacaa acguucccuc ugaguacgaa aaaggguuca agaaaaagcg gauugaccac     3180

cgccaucacg ccauggacgc ucuugugauc gccugcacca caagaaauau ggucaacuau     3240

aucagcaaug ugaacgcuaa uuccccuaaa caacgggagg aucugagaca gcuccugugu     3300

gacaagaacc gcauuaucaa uaagcccugg aacaccuuua cccaggaugc ccugaaagcg     3360

cuuaaugaca uuguugugag cuucaagaac aacguccgga ucauuaauag agccacaaac     3420

cgcuaccaac gguaugauaa aaauggcaag aaaaugaucu gcucucagaa gggaaacgac     3480

augugggcua ucagaaaguc caugcauaaa gaaacggugu uuggccgcgu gaaucugauu     3540

cggaaggagg cccucccgau cgcuaaagcc cuggauaaca uuagcgccau cuguaaugcu     3600

cagcucagag cguacgucaa cgaccugguu gauaagcacu ucaauaaaaa gcagcuugug     3660

gcccauuuua agucccugga cuauaaaugg aaccgccaag augugucuaa ggucgaggug     3720

uggauuagcu ccgacgauaa aaccccuaug guugcugugc ggaagccccu cgacacaucu     3780

uucgacaaaa agaagaucgc cagcauuacc gauacaggga uccaggccau ccugcugaau     3840

uaccuugaau ccaaaggagg cgacgcucug gucgccuuug cgcccgaggg gauucaggaa     3900

augaacucua auaucgagcu cuauaacaau ggaaaggcuc acaaaccuau uaagaacgug     3960

agacugagcg aaacccucgg cgccaaauac cagguugggg agcggggcgc caagacgaaa     4020

aaguacgugg ucgcugauaa gggaacaaau cuguucuuug ccaucuauga ggacgaagag     4080

ggcaaacgcu acuauuacuc cgugccgcuu agagaagcga uugagcggca aaagcagaaa     4140

cugagccccg uuccugaaua uaacgagaag gggguggcuc ugaaauucua ccucucuccc     4200

aacgaucugg ucuaugugcc uaccgaggag gaacgcacca ugggaacaug ccacaucgac     4260

aagaccagaa ucuacaagau gguguccagc ucugaaaauc gguguugcuu cguccccucc     4320

acaauugcca aaguuaucgc cgauaaggug gagcuucuga gccauaacaa aauugaaaag     4380

gcuaucacgg gcgagucuau uaaagaggug uguuggaagc ucaaggucga cagacugggg     4440

aauauccuca acgugaucaa auga                                            4464


<210>  4568
<211>  4464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4568
augaagaaca uccugggccu cgaccugggg agcaauuccg ugggaugggc ccuugucaaa       60

guugaggaaa acggcaaucc cauugggucu aucaagaugg gaagcagaau uaucccuaug      120

ucccaggaua uucugggcuc uuucgagaaa ggggacaccg ugagccaaac agcucagcgg      180

acgcucuacc gcggaaagag acggcugauc gaacgccacg ugcuuagacg ggagcgccug      240

ugcagagucc uccauaucau gaacuuucug ccggcccacu ucgaucggcu gcucggcugg      300

gacaaaaccg auaauaagac auauggcaag uuuauugacg auuccgaacc caaacuggcg      360

uggcgccaga acaagaauga gauggggaaa auggaguucg uguuuaugga cucuuuccau      420

gaaaugcuua gcgauuuugc uaagcaccag ccucaacuga ucgccaacgg caaaaagguu      480

ccccucgacu ggaccauuua cuaucugaga aagaaagccc uuacacagcu gaucuccaag      540

gaggaacucg cuuggauucu gcugaauuuc aacaaaaagc ggggcuacua ucagcuccgc      600

ggggaggagg aagaggaaca gccuaccaaa aaggaggaau acaaagugcu gaaggucauc      660

agcguggaug ccgacgaggg acaaaagggc aaugggauuu gguauaacau ccaucuugag      720

gauggaggca ucuacaaaaa gaaaucugac auuccccugu acgacugggu uggcaagaca      780

cuccagcuga ucguggucac cacguaugaa aaagauggga agaccccuaa gauuuuugac      840

uccggaaaac agaauccgag cuacagacuu cccaaggagg augacugggg ccuggugaaa      900

aagcggacag aaucucagcu ccaauccagc gggaaaaccg ugggagcuuu caucuaugau      960

aacauucugu cuaagccuga cgauaagauc cgcggcaauc uggucagaac aaucgagcgg     1020

aaauacuaua agaaagaacu cauugagauc cugcagcagc aagccaagua ccacgacgag     1080

cuucacaacc agaaccugcu cgaauccugu gcgaaagagc uguaugccca uaaugauguu     1140

cacuacaacg aagugauuaa gagcaauaug aucaaccuuc uggugaauga cauuaucuuu     1200

uaccagagac cccucaaguc caaaaagucu cugaucgcua acugcaaaua ugagagcuac     1260

gaauaugucg auaaggagac cggggagauu aaagaaaugc cuaucaagug uauugccaaa     1320

uccaaucccu acuaucagga guuccgccug uggcaauuua uccauaaccu ccggcugagc     1380

gacgugguua cgagagaaga cgugacagcu caguaccuuu cuaccccgca ggauuauugc     1440

aaucuguuca cauaccucaa cgaccgcaag gagauuaagc aggauauccu gcugaaagac     1500

uucuuuaaga uuaaaaaggu ccaaaucgau aaagagaagg uguucccuau cgaauggaau     1560

uacauuaagg acgccaccaa auccuauccc uguaacgaga cccgguacga acuucucagc     1620

gcgcugaaga gagccggaau ggauaaacag uggcuggacg auacaccuaa uaugcaguau     1680

cgccucuggc aucugcuuua cucuguugag gaaaaggagg agagcgcuaa agcccugcgg     1740

aagcucuaug acgaugacaa guuugugacg uccuuccuga aaaucaagcc cuuuaacaaa     1800

ugcuacggcg cuuauucuga aaaggccauu aaaagacugc uuacccucau gcgcaugggc     1860

aaggccugga augagcaggc uaucgaugcg aagacacuga aauguauuca acagaucauc     1920

accgacaacg ucgacagcaa gcugaaagaa aagauugaug acucccggua cgugcuccac     1980

cacgucagcg auuuccaggg gcugcccaca uaccuugccu cuuaugugau cuacggaaga     2040

cacuccgagg uuaccgacau ucagugcugg gauagcccug aagaccugcu caaauuuauc     2100

cgcgauuuca agcaacauuc ucugcggaac ccgauugugg agcaggucau ccuggagacg     2160

cuuagagugg uuuaugacau cuggaaagcu gaaaagcaca uugaugagau ccauguggaa     2220

gucgcccggg agaugaagcu cacagcccag caacgcaaug aagcuaaccu gagaaaucug     2280

aacaaugagg ccaccaaccu ccggauuaaa uaccugcuug aggaacugaa gaaugacacc     2340

uuuaucaaag augugagacc ccucuccccu auucaccagg agaagaugcg caucuaugaa     2400

caggcggugc ugggcaaccu gaauaaacag gacaaggauu acgacgacau cauuaagauc     2460

agcaaaaagg agcaccccac aaacgcugag cuuauucggu auaaacucug gcuggaacaa     2520

agauacuguu ccccuuacac cgggaaggcc aucucucuga gcaaacucuu cacaacggcc     2580

uaugagauug aacauaucau uccccagucc cgcuacuuug auaauucuuu caacaauaag     2640

guuaucugcg aggcugaagu caacaaggcc aaaggaaaua ugcugggcua ugaguuuauc     2700

aagaaacaag ggggccagaa gauuuacugu acccagcuug gaaaagcugu gacaauccug     2760

aaggaggaag ccuauaagca guucgugaac gagcacuacg cgcauaacaa aagaaagcgg     2820

gacaaucucc uggccgaaaa cauuccggag agcuucucca gccgccagcu gaaugauacc     2880

agauauauca caaaaaccau uaugucucuu cuguccaacg ucgugcggca gcaaagcgaa     2940

gaggaggcua cggccaagaa ucuccugccu ucuuccggca gcguuaccga ccgccucaaa     3000

aacgauuggg ggcugaagga cguguggaau acaaucguca cccccagauu ugaacggcuu     3060

aacaagcugu cuggaacaaa ugauuucggc gagaugaaag aagagaacgg gaaucgcuac     3120

uuucagacca acgugccucu cgaauacgag aagggauuca aaaagaaaag aaucgaccac     3180

cggcaccaug cuauggaugc ccugguuauu gcgugcacga cacgcaauau ggugaacuau     3240

aucagcaaug ccaacgcuaa cucccccaag cagagagagg accugcggca acuucugugc     3300

gauaaaaaua gaauuaucaa caagccuugg gacaccuuua cacaggacgc ccucaaggcu     3360

cugaaugaua uugucgugag cuucaaaaac aauguucgca ucaucaaccg ggccaccaau     3420

agauaccagc gcuaugacaa gaacggcaaa aagauuaucu uuucucagaa aggggaugac     3480

augugggcca uucggaaguc caugcacaag gaaaccgugu ucggcagagu caaucucauc     3540

cgcaaagagg ugcugcccau ugcuaaggcg cuugauaaca ucagcgccau uuguaaugcu     3600

caacugcggg ccuacgugaa cgaccucguc gauaaacauu uuaauaagaa acagcugguu     3660

gcccacuuca agucccugaa cuauaagugg aauagacagg acgugucuaa aguggaaguc     3720

uggaucagcu ccgauaacaa gacaccgaug guggcuguuc gcaaaccucu ugacacgucu     3780

uuugauaaga aaaagaucgc cagcauuacc gacacaggaa uccagaccau ucugcucaau     3840

uaccuggagu ccaagggcgg ggaugcgucu guggcuuucg cccccgaggg aauccaagaa     3900

augaacagca acauugagcu cuauaauaac ggcaaagccc auaagcccau caaaaauguc     3960

cggcuguccg aaccucuugg ggcuaaguac caggugggag agagaggcgc caaaacaaag     4020

aaauacguug aagcggacaa ggggaccaac cuguuuuucg cuaucuauga ggacgaggaa     4080

ggcaagcggu acuauuacag cgugccgcug cgcgaggcca uugaaagaca gaaacagaag     4140

cucucucccg ucccugagua uaaugaaaaa ggaguggccc ugaaguucua ccuuuccccc     4200

aacgaucugg uuuaugugcc uacggaggag gaacggacaa ugggaaccug ccacaucgac     4260

aaagcucgca uuuacaagau ggucagcucu accggguguu ccugcuacuu ugugcccagc     4320

acauucgcca aggugaucgu cgauaaaguu gagcuccaau cucugaauaa gauggaaaaa     4380

gcuauuaccg acgaguguau caaggaagug ugcuggaaac ucaaggugga uagacuggga     4440

aacaucauua augucaucaa guga                                            4464


<210>  4569
<211>  4485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4569
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugggc ccuuaucgau       60

aacgaguccc acagaauuau caaaucuggc agccggauua uccccaugga cgcugccaca      120

augggggauu acgaaaaggg aaaucugaaa uccgcggacu ggaagcgcac gucuuucaga      180

ggcaugcggc gccucuauga gagagcuaaa cugcggcgcg aaagacuucu gcgggugcuc      240

aacauccugg gguuucugcc ugaggaguuc cgcagacaga uugauuuuga acaucacccg      300

ggacaauuca ucgacggcaa ggagccccuc auugccuacc aucacgauga aaauggccag      360

cgggucuuua gcuauauguc cgccuucgag gaaaugcuga ccgacuuucg cgcuacacag      420

ucugagcuug uuagagaugg gcggcgcgug ccuuacgacu ggaccaucua uuaccugaga      480

cauaaggccc ucacacagcc cgugaccaaa gaggaacugg cuuggauuau ccuuaacuuc      540

aauacaaagc ggggauauua ccaacugaga agcgaguccg aagaggucac cacgaacaaa      600

aaugaagagu acgccgagcu caaggugguu agcguggaaa agaccggcga ggauaagaag      660

cgcgcggggu auuacuggua uacaauuacc uacgacaacg aagccgagca gaaaaucaca      720

ucuaccaucc cuccccggca gauuggagau aaggucgaac ugaucgugac gacagcuaga      780

gacaaagccg gcgagauuaa gaaauccguu cgcagcccua aggaggacga uuggacccug      840

augaaaaagc ggacagaaca caauaucgag gaagaggaau gcaccguggg gucuuauauu      900

uaccagcaca uccucgcuga cccgaccguc aaggugagag gaaaacuggu gcauacaauc      960

gagcgcaagu uuuauaaaaa ggagcuuaaa caaauucuga acaagcagaa ggaguucauc     1020

cccgaacucc aggaugccuc ccuguacgaa gcguguauua aagagcuuua ccggaauaac     1080

gaggcccaca uccagagcau ugcuaauaag gacuuuacgg aucuguucgu caacgacauc     1140

aucuuuuauc aaagaccucu caaaucuaag aaauccacca uuagcaacug ccccuacgaa     1200

uccuauuacu auaagaauaa ggagacaggc gaacugaucc gcaaaccuau uaaguguauc     1260

gccaaaucua acccccugua ccaggaguuc cggcucuggc aguucguuca uaaucugaga     1320

auuuauaaga aaagcgaaga gguguccggc cgccuucaaa ccgaugugga cgucacagau     1380

cgguuucuga ccacgccgga cgauuacgcu aaacucuuca gcuggcugaa cgacagaaag     1440

gagaucaaac agaaggaacu ucugcgguac gaggccuuug ggcucucuaa auccuaugga     1500

gacuaccgcu ggaauuucgu ggaagauaag ccuuaucccu gcaacgagac acugcacgaa     1560

auuaauagac ggaugaaaag cauccugucu gacaagaaac ucagcuccga ggccuaccag     1620

cagcuguggc acauccuuua uucuauugau gacccuaucg agauuagaaa ggcucugacc     1680

agcuuugcgc aaaaccagga aaucaauucc gaugacuucg uuaacgccuu uuaccaguau     1740

gugcccgaga agaccaaaag cuacggcgcu uacucugaaa aggccauuaa aaagcuccug     1800

ccccugaugc gccggggguc cuauugggcc aucggaaaua ucgaugagaa aacacauaga     1860

cgccuugaca acauuaucaa uggcgucgcu gaugacagca uuucuaccug uacaagagaa     1920

aagcucaaca aucugacgga uaucaccaug ugccagggga ugccucugug gcaagccugu     1980

uacgcggugu augaccggca cuccgaggcu gccgauacaa ccaaguggga gcagccggac     2040

gacauugaua cauaccucag caccgaacug aaacagcauu cccuucgcaa ccccaucguu     2100

gagacgguga ucucugaaac ccugagaguc gugcgggaca uuuggaaggc cuauggacgc     2160

ccugaggaga uccacgucga acucggcaga aaccugaaac aggaugcuaa gagccggaaa     2220

caagacgcca uucgcauggc ugagaaugaa cagacaaacc ugagaauccg ggcccuucuc     2280

caggagcugu ccaaucccga augcggcgug gagaacguuc gcccucauuc ucccagccag     2340

auggagcugc ucaagauuua cgaaaaugag gugcugcaau ccaccgauga caucccggaa     2400

gagaucagca acauuaucaa ggaucuuggg aaaacagacu cuaagaaaag accuucccug     2460

agcgauauua ccaaguaucg gcucuggcug gaacagaaau accagucucc cuacacggga     2520

cacaucauuc cucuguccaa acuuuucaca cccgcguauc aaaucgagca cgucauuccu     2580

cagagcagau acuuugacga uucuaucagc aauaagguga ucugugaggc cgaaguuaac     2640

aaagagaagg accgcaugcu cggcuaugag uucauuauca auaaaggggg auccauugug     2700

aagggcagcu uugggcagca guucaaaauc uuugauaagg uccaauacga agacuucgug     2760

aaacagcggu augcucauaa caagaccaag augaaaaagc ugcugaugga cgauauuccc     2820

gacggcuuca uccagagaca gcucaaugau ucucgcuaca uguccaaaaa gaucauuagc     2880

auccugucca acauugugcg ggaagacgga gagaaagagg ccacaucuaa gcacaucauu     2940

guuaccagcg gcgcuaucac cgauagacuu aagaaagaau gggggaucaa ggacgucugg     3000

aaugauauua ucgccccgcg cuuuaaacgg cugaacaagc ucacagacuc caauaacuau     3060

ggaauggaga cggaaaaaga gggcagacaa uacuuccaga uuaacgugcc uaccgagcug     3120

ucuacagggc ugaucaagaa gcgcauugau caucggcacc acgcgaugga cgccaucauu     3180

aucgcuugca ccacaagaaa ucaugugaac uuucuuaaua acagcaccgc cgcuuccgau     3240

cagaaagaca uccggcgcga ccuccagcac acgcugugua caaagaccaa aaccgauucu     3300

aauggaaacu acgucuggag auucaauaag cccugggaca cauuuaccca agauacacgg     3360

caggaacugg agggcauuau cgugagcuuc aaacagaacc uccgcauuau caauaagaug     3420

uccaacuauu accaacauua uguugacggg agaaagacga uuagccggca gaauaaaggc     3480

gauggauggg ccaucagaaa gucucugcac aaagccaccg uguccggcaa ggucaaccuu     3540

aaacgcagca agacaguggg guuuaaggaa gcucugaaug acuggucugc gaucaaagau     3600

aagaccucca aagccauucu ccagaagauc auugcucagu acaaaaacua ugaccccaag     3660

acaaucuuca aauacuuuaa ggaucggaau uucaagguug agggaaaaga cuuuagcaag     3720

cuggaagugu auuacaacac cucugauaaa gagccuaugu ccgcccauag agucaacauu     3780

gacacgaccu ucgaugagaa ggaaauccgc agcaucacag acaccggcau ucaaaagauc     3840

augcugaagc accuucacac auacgacgau gcccagggga agacccaucc ggaggcugcc     3900

uuuucccccg aaggaauuga gaaaaugaau cagaacaucc ggaugcugaa uggcgggaag     3960

gaccacaaac cuauuuucaa ggugagaaaa gcggaaaucc ucggcaugaa guucgcuguu     4020

ggagagcgcg gccauaagga uaaaaagugg guggaggccg acaaagggac aaaccuguuu     4080

uucgccauuu augaaagcga ggaaaauaag cggucuuaca gcuccauccc ccucaacgag     4140

gcuaucgaua gacugaaacg cggagaaaau auugccgacg agagagauca gaacggcaau     4200

aaacuucugu uuguccucuc uccuauggac cugguguaug ugcccgaucc ugacgaugcg     4260

cacaucgacg aggucaaggg gaacaauaac auuuucaaga ugguuagcug caccggaacg     4320

gaaugcuacu uugugcccga gacauaugcu aaugugaucu guaagggcaa agaacuggag     4380

uccaagaaca aagucgaguc uuacgauggg auuaauauca agaaaagcug cuauaagauc     4440

accguggacc ggcuuggcaa gguucgcaaa auuuucaagg gauga                     4485


<210>  4570
<211>  4491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4570
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc caaagugugc       60

gucgaugaca auggcaacua ccuuagagau aucaagcugg ggucccggau uauccccaug      120

ucucaggacg uucucggaaa uuucgauaaa ggcgugacgg agagccaaac cuccgugcgc      180

acaggguuua gaggaguccg gcgcauuaga gaacggucuc ugcuucgcag agagcggcug      240

caccgcgugc uccauguucu gggcuaucug ccuccgcacu acgacagcgc uaucggcugg      300

aacaagaaug aacccuccac cuaugggaaa uuccucaacc auggagagcc uaagcuggcc      360

ugggaacaca aggaggaugg cucuaugaga uuucuuuuca uggagagcuu ucaugaaaug      420

auggcggacu ucgcuaaauc ccagcccagc cuggugucug augggaagaa aaucccucuc      480

gacuggacaa uuuacuaucu gcggaagaaa gcccuuuccc agcccauccg caaggaggaa      540

cuggccuggc uccugcugaa uuuuaaccag aagagaggau acuaucaacu ccggggcgag      600

gaggaugacg auaauccuac caaaugugaa gaguacuacg aacugaaggu cguggggauu      660

gaggcugacg aagagggaaa gaacggcaau acaugguaua acguuaaacu ugagaauggc      720

uggaucuacc gcagacagag caaaauuccg cuggacgauu ggaucgggaa ggugcgggag      780

uucauuguca ccacggaaua ugaaaaagac ggaaucaccc ucaagaagga uaaaaacggc      840

gacgugaaga gaucuuuucg cucccccagc gaggaugacu gggggcugcg gaaaaagaga      900

acagaaaaac uucuggauuc uuccaagaug accgugggag ccuucgucua cgaccaccuc      960

cuggcugagc cuagcgauaa gauccgcggc aaauuuauuc ggacaaucga gagaaaguau     1020

uacaaaaagg aacuguaugc cauucucugc gagcagucca aauaccacga ggaacugcgc     1080

ucuggggacu accuuaauaa gugugcggag gaacuguaua agaacaacca gccccaucgg     1140

aauagccucc ugaccaaaga ucuugccuac cugcuccugg aggaccugau cuucuaucaa     1200

agaccucuca agacgaaaaa gucccugauu gcugaaugcc ccuacgaggc cuauaaauac     1260

guugacaagg aaacaggaga gaucaagcgc cagggcauca aauguauugc uaagagcaac     1320

ccguauuacc aggaguuucg gcuuuggcag uucaucucua aucugagacu cuucaacaaa     1380

gccgaugaca aggaagugac cgcggauuac cugccuacac aagacgauua ugugcgccug     1440

uuugccuacc uuaaugaccg gaaagagauu aaccaggaaa cccuccugaa ggaguucuuu     1500

aaacugaaga agaucaccgu caauuccgaa aaaguguucc ccauuagaug gaacuauauc     1560

gaggauaagg agaaaaagua cccuugcaau gaaacacggc acgagcuccu gcuugcucug     1620

gaccgcgcca gaaucgauca uagcuggcuc gaaaaacccg gccaggagua ucggcugugg     1680

caucugcuuu acucuguuga aagcaaggag gagacgaagg aagcucucag aaaacugaag     1740

gacgaugacg ccuuugugga guccuuccug aaagucaagc ccuuuaaacg cgaauauggg     1800

gccuacucug agaaggcuau uaagaaacuc cuggcgguga ugcggauggg aagccuuugg     1860

agagaaaccg auaucuguga cacaaccaag ggcaacauua caaaaauccu gcaaggggag     1920

auugacgaga agcucaaaga aaagaucucc gccagcguuu cuuccuacaa ucagcugagc     1980

gauuuccagg gacugccugu guggcuugcu ugcuaugucg uguacggccg ccacucugag     2040

gccgccgaaa uucagcacug gaagaccccg gagucccucc uggcuuauau caacggguuu     2100

aaacaacaca gccugagaaa ucccaucguc gagcagugua uucucgaaac gcugcggaca     2160

gugcaugaca ucuggaagga gguuggccac auugaugaaa uccaugugga gcuuggacgc     2220

aacaugaaau ccaccgccga ccagagagcg cggauuucug aaaauguccu gcgcaacgag     2280

aacaccaauc ucagaaucaa gagccugcug cuugagcuca aaaacgaucc uaauaucaag     2340

gacgugcggc ccuacucccc uaugcagcaa gaaaaacugc gcauuuauga ggaaggcguu     2400

cugcaggagc ucacaaagga ugacaaggau uucaccgaaa ucacaaaaau uucuacgaug     2460

gcucagccca gcgccaagga gcugcagaga uacaaacuuu ggcuggagca aaaguaucgg     2520

uccccguaca ccgggaaaag caucucucuc acaaagcugu uuaccuccgc cuaccagauu     2580

gaacacguga ucccucagag ccgcuauuuc gacgauucuu uuuccaacaa ggucaucugc     2640

gagagcgaag ugaauagccu gaaagacaac augcuuggau acgaguucau uaagaaucac     2700

ggcggggaaa ucauucauug uacaaugcug ggagauguga aaguucucag cgaguccgag     2760

uauaaggcuu ucgucaacga ccacuacgcc cauaauaaaa gaaagcggga aaaccugcuc     2820

cuggaggaca ucccccagga auuucuuaau agacaaauga acgauagccg cuauauuucu     2880

aaggugguga aagcucugcu cuccaauauc guccgggccg agggcgagga agaggcgacc     2940

agcaaguucg ugguuuccug cucugggggc aucacggaca gacugaaaaa ggauugggga     3000

cugaacgacg uguggaauaa aauugucuac ccucgcuuug aacggcuuaa cagacugacc     3060

ggcacagagg ccuucgggca gugggaaaac aaggauggaa agcgcguguu ucagaccgcu     3120

guuccccucg agcugcaacg gggcuucaau aaaaagagaa ucgaccaccg ccaccaugcc     3180

auggaugcuc ucgugauugc cugcacaagc cggaacauca uuaauuaucu gaacaaugag     3240

uccgcgaaca auccuaaaaa gagagaagac cuucggcaga aacuguguga uaagaaccgc     3300

accaucagaa agcccuggga gacguuuaca caggacgcca ccgcugcccu cgaagauguc     3360

auugugucuu ucaaaaauua cguucggauc aucaacaagg cuacaaauua cuaugagcgc     3420

uacgacgugg aagggaaaaa ggucauugcc gagcagagag gagacgauau gugggccauc     3480

cggaaaccga ugcacgaggc uaccuauuac ggcagaguga accugcgccg gaagaaaaag     3540

gugaaucuga gagucgcgcu ugaaaacauu agcuccaucu gcgacaauaa gcugcgcauu     3600

uauaucaacg ggcucguuga gaaaggcuuu aacaccaagc aaauccugga uaaauucaag     3660

aauacaaacg accauuuuuc uaugcagaaa auugauaagg uggaaaucug gcaguucacg     3720

aaugaggaag agaagcucgu ggccacccgg aaaccucugg acacaucuuu ugauaccaag     3780

aaaauugcuu ccaucacaga caccggcauu cagacgaucc uucugaacua ccuggagaca     3840

aagggagggg auagcaccau cgccuucacc cccgaaggaa uugucgagau gaaucaaaac     3900

aucucuaaau auaauaacgg caaggaacac augcccauuc ucaaggugag acuguacaca     3960

aaacuuggga agaaaauccc uguuggacag accggcugua aggccaaaca guucgugauu     4020

gcugaggccg ggacaaaucu guacuuugcg aucuaugagg acgaagaggg caagcgcucc     4080

uaccggacga uuccgcucaa cgaagucgug gagagacuga agcagggacu cagccccguu     4140

ccugagaaaa augauaagga cgugcaucug aaauucuauc uuucucccaa cgaucugguc     4200

uacgugccua ucgaagacga gcacgaaucc cuggcuaagg accacaucua uaaauuuguu     4260

gauagcucug gcaccacagc caauuucauu ccccaucgcu ccgccaacgu gaucuuugag     4320

cucaauaaga aagacgcuga aaaguucugc agcgggucca uuauccaaaa cgaguacgga     4380

gucggcagcu cucagagcaa gaaucagaaa gccauuaccg gggaaaugau caaggcggug     4440

uguagaaaac uggaggugga ucggcuugga aacgucauca agguuauuug a              4491


<210>  4571
<211>  4497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4571
augaagcaca uccugggccu cgaccugggg accaacagca uuggaugggc cgugaucaga       60

gcucaucagg augccgacgg ccacacaacg cuucaagaua uucuggcggc ugggucccgg      120

aucauuccca uggacgccgc cacccucaca gauuucgaca aaggaaaugc ucgcucuggc      180

accgccgaga gaaccagcuu ucgguccaug cgcagacggc gcgaaagaca gcugcuucgg      240

cgcgagagac ugcaucgggu ccucaugcug cugggguucc ucccucagca cuacgcuaac      300

ucucugacac gcuauggaga auuugccgau aauagagagu gcaaacuucc guggguuaaa      360

gacgaaagcg auggcaagua ccauuuccug uuucaggacg cguuccacga gaugugugag      420

gauuuucggc aacucgcucc ccagugggug gcugacggca aaaaggugcc uuaugauugg      480

acacuguacu aucuucgcug caaggcccug uccagaaaaa ucucuaagga ggaacucgcu      540

uggauccugc ugagcuucaa ccagaaacgg ggguacuauc aggccagaga cgaagaggac      600

gaggucacca cgaauaagcu cgaagaguac uacgcgggaa aagugguucg cguggaugac      660

accggcgaac ggaaggggaa gaacgucuau uacgauauua cacuggagaa uggaugggug      720

uauagauacg ccgcucgcau cuaucccgac uggguuggcc aagugaaaga auuuauuguc      780

accacaaccc uuaacccuga ugggacgccc aagacagaca aagccggaga ggugaagcgg      840

uccuucagaa ugccuaaaga ugacgauugg acccugauca agaaaaagaa ggagcgcgac      900

auugaucgga gccaggaaac aaucggccag uacauuuauc acgcucuccu gaaagacccg      960

ucucagaaga ucagaggcaa acuuaucggg accguggagc gcaaguacua caaagacgaa     1020

cugcggcgga uucucgaucg ccaaauccag uuucaucccg agcugcggga cagagaacug     1080

uauuacgccu gucuccagga gcuguauccu uccaaugucg ccuuccggaa cacccuuguu     1140

aaucgcgauu uuacauaccu guuccucgac gauauucugu ucuaucaaag accccuuaag     1200

agcaagaaau cucagaucuc cgacugcccu uacgaggaac acuuuuauac ggauaaggag     1260

accggagaac ggauuagcgu gcccgugaaa uguaucgcug ucucucaucc gcuguaccag     1320

gaguacagac ucuggcaguu cauccaaaac cugcgcauuu auugccggga agcgagaauc     1380

gacggcaauc ugaugauuga uugggaggug acaaacgagc uccugcagac cgaagaggac     1440

aagacagccc uuuuugauug gcugaaugac aaagaauccg uuaagcagcc ugagcucgug     1500

gcuuuccugc ugggaggaaa ggccaacaaa aagcagauca aagccuaccg cuggaacuau     1560

auggaugacg acaccaaggg cuaccccugu aaugagacga gagcuagccu ucucaaauau     1620

cugcgggaag cccaaacaga uuccucuuuu cugacccgcg agagagaaga ggcgcucugg     1680

cacauucugu acagcaucuc cgacaagggg gaacuuaaga gcgcucuggg aaaauucgcc     1740

gucaagcacg gccucaacga gcgguuugug accguuuucu cuaaauuucc ucccuucaag     1800

aaagauuaug gcuccuacag cgccaaggcu cugaaaaagc ugcuuccccu caugcgcaug     1860

gggcaucacu ggcaggaggc cucuuuuauc ccuagcacaa gacagcggau ggaaaaguuc     1920

auuaauggag aguacgacga uuccaucgac aaacgcaccu augaaaagcu ggagucucug     1980

acagaaguga gccaguacag aggccucccg ggguggcugg cuugcuaugu cguguacgau     2040

cggcauuccg agggacgcga cauugagaga uggacgagcc ccucugauau cgacuccuau     2100

cuugcccaau uuaaacagca cagccugcau aacccuauug ucgaacgggu gguucucgag     2160

acccugagaa cagugcgcga uaucuggaag caggucgacc acauugauga aauccacauc     2220

gagcugggcc ggaauaugaa acuucccgcg gacaagagac gccagaugac cgaacaagcc     2280

uggaagaacg agcagacaaa ucuccggauu aaagcucugc ugcucgaguu cgccagaccu     2340

ggguacgaca ucgaaaacgu gcgccccuau ucuccguacc agcaagauca gcugcggauu     2400

uacgaggaag cuguucuugg cgccaaugag uguccugacg auaucuccgc gauucugaag     2460

aaauucaacg gacucaaucc cccuaccagc gccgaggugc ugagauauaa gugcuggcug     2520

gaacagaaau accgcucccc cuauacgggc cggaccaucc cucuuucuaa gcucuuuaca     2580

agcgcuuacg agaucgaaca uauuaucccc aagcagagau auuucgacga uuccuuuucu     2640

aacaaaguca uuugugagag cgaagugaau aagaaaaagg gggccaugcu gggauacgag     2700

uucaucaaau ccuaucacgg cgcuagcauu acccugaaca gagggggaac agccucuauc     2760

uuuuccgugg aggccuacga aaauuucguu aaggagcauu acgcucacaa ccgcggcaag     2820

augaaaaagc uccugaugga cgaugucccg gacagcuuua ucucuagaca acuuaacgau     2880

ucccgguaua uuagcaaacu gaugaagucu auccucagca aucuggugcg cgaagagggg     2940

gaaaaagagg cgauuuccaa gaaccugauc gugugcaccg gcggaauuac ggacagacuu     3000

aaaaaggauu ggggcaucca cgacgucugg aaugacauca uuuucccucg guuuaagaga     3060

cuggaugaga uggacagcuc ucauacauac aucacccuca acacacaccg ccaucccauu     3120

cccuccaugc cucuggaguu ccagcggggg uuuagcaaaa agagaaucga ucaccgccau     3180

cacgccaugg acgcuauugu gaucgccugc gccacccgga auauuguuaa cuaucucaau     3240

aacgaauccg cuagacacga ugccaaaacc ucucgcaugg accugcagcg gcuucugugu     3300

acagcgaaua gaaagcucau cgauaaaccg uggaagacgu ucacccagga cacaugcaag     3360

gugcuggaag auaugaucgu cagcuuucaa cgcacccuga gaauuaucaa caaaacaaag     3420

aauuacuauc uucgguucga ggacggaaag aagcgcagag ugcugcagac caaaggcgau     3480

aacugggcua uucggaagcc ccuccauaag gaaacguucu acggggagau caaucugcgc     3540

agaauugaga cacucucccu gaacaaagcc cuugaacgga ucccugacau cguugagaga     3600

gaucugcgca agaaacucuc ugaacugcug agccuuggau auaaugccaa gcggauuaaa     3660

ggcuacuuug aggagcacaa ggaagagugg uccgacguga accugucuaa gaucaaagcc     3720

uacuauuuca ccaagcagaa aaacaagaga uacuuugccg cgcgcaccga acucgacacc     3780

agcuucaaua aagauaagau ugagaacaag aucgcugaca cagccauuca gaaaauccug     3840

cuccaacauc uggaagccua ugggaaugau gucgagcagg cuuuuucccc cgagggcauc     3900

gacacaauga accggaauau uagacagcuu aacggaggcc ggagccacca accuaucuac     3960

aaggugcgcg uuuaugaaga ggccacgaaa uucgcugugg ggagacgggg aaauaagucu     4020

accaaauuug ucgaagccgc gaagggcaca aaccuguucu uugccauuua cgagaucucc     4080

cagguggaug ggaauggaaa ccgccagacc agcagaacau ucucuuccau uccccugaau     4140

guggcugucg aacggcucaa acagggccug ccucccguuc cggaggacga ggaagggaac     4200

gccccuaagu augugcuuag ccccaaugau cugguguacc ucccuaccga gaaggaacug     4260

aaacgcagag agaucgcuau gcccauugac aagucucgga ucuacaaaau ggucuccugu     4320

acgggcaagg auugcuuuuu caucccggcc aacaccgccg ugccuauugu ugaccgcuau     4380

gaauacagcu ccaugaauaa aaucgggaga gcucucacag gcgagaugau uaaggagauc     4440

ugucugcccc uuaagauuga ucggcugggg caaaucaucg aaauuagcac cuucuga        4497


<210>  4572
<211>  4506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4572
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cgugaucaau       60

aaggcugaga acgaaggcaa agauguccuu ugcgggauug aggccgcggg auccagaauc      120

auucccaugg acgcuacaau caugggcgau uucgaaaagg gaaauucugu uagccagacg      180

aaagaccggc gcgagcacag auccacccgg cgccuguacg aaagagggaa gcuccggcgc      240

gagagacuga accgggugcu uucugugaug ggcuuucugc cugaucauua ugucagccaa      300

cucgaccgcu acggcaaguu ccuguccaau acagagccga aacuggccug ggugagaaac      360

gccgauugua aguaucacuu ucucuucaug gacucuuuua gcgaaaugcu gcaggaguuc      420

aaaaccaaua gcccccagua caucagcgaa gagucuaagg uuccuuauga uuggacacuu      480

uacuaucugc ggaaaaaggc ucucacccau aaggugacaa aagaggaacu ggccuggauu      540

cuucugaacu uuaaucagaa gcgcggguac uuccaacuca gagacgaguc cgaagaggag      600

aaaaagccca gcaaaauccg gcaguacuuu gagcaccaga aggucgugga uauuaucgac      660

accaacgagg uuuauaaagg acugaaggug cugauuguca uccucgcuaa uggcauuucu      720

gggaagaucu ucaaaaagga aaucccugau uggauuggag uggagaaaga caucauugug      780

aaggucgaca ucgauaaaga cggcaaggau aaguuuaacg aagacggguc ccugagcugc      840

cgcuucaaua uucccacgga ggccgaaugg gauaccgagu gggagcuuau caaacagaag      900

acacaaaacg accuggaugc gucuggaaaa accguuggcg aauacaucuu ugacacacuc      960

cugagaaauc cugagcagaa gauucggggc aaacuuguga gaaccaucga acgccgguau     1020

uacaaggagg aaauuaacgc cauccugaag aaacagaagg aguuccaucc ggagcucucc     1080

gauagagcuc uguaugaaaa auguguggag gaacuguacc ccaacaaucc ugcccaccgc     1140

aacaugaugc ucauucacga ccugagcuau uuccuuguca augauauccu guuuuaccag     1200

cggccccuca aguccaaaaa gucucugauc gacgagugcc cuuacgagag ccaucuuuau     1260

auugacaaga aauccgggga aagaaagccc guggcuguua aauguaucgc caagagccac     1320

ccgcuguacc aagaguuccg ccucuggcag uuuauuucua accugcggau cuaucagaaa     1380

acgaaggaag ugggaaauuc ccugaaaguc gauguggacg uuacaaacga guuccucaag     1440

ggcgaaaaug auuacgugga gcuguuugac uggcuuaaca ccaagaaaga gauuaagcaa     1500

gaagauuucc ugaaauauaa gccuuuuggg uucacaaaag gaaauaucaa caauuacaga     1560

uggaacuaug ucgaggacaa gauuuacccc ugcaauaaga cccauuacac cauccucaaa     1620

ggccuggaaa aggcggauau cacauuugac gaguucauua gccacgauuc ucuggccgaa     1680

gagaaccuuu ggcacauccu cuauagcgug uccucuaaag aggaacugaa gcaugcucug     1740

gagagcuuug ccgacaaacg cgggcucucc auuaaguucg ucgaugcuuu uaucaauacg     1800

ccugccuucg aaaagagcua cggagccuau ucugcuaaag cgauuaagaa acugcuuccc     1860

cugaugcgga ugggcaagua cuggaaagag gaaaacaucu gugacuccac cagagagcgc     1920

aucgauaaga uuaucacagg cgaguaugac gaaaacauuc gggacagagu gcgggaaaag     1980

agcaucaauc ucaccgauau uacacuguuc cgcgggcugc cccuuuggcu cgccugcuac     2040

guuguguaug acagacacuc ugaagcugga aacaccgagc gguggacguc cccuagcgau     2100

aucgaccauu acaucagaaa auuuaagcag cacucccugc gcaauccgau ugucgagcag     2160

aucgugcugg aaacacuccg gaccguuaga gaugugugga ccgaggucgg cgggauugac     2220

gaaauccaug uggagcuggg acgcgaaaug aaaaaccccu cugauaagag acggaaaaug     2280

agccuucaga ugcaagagaa ugagaacaca aaucugcgca uuaaggcccu ccuggccgag     2340

uucaucaacc cugagcuggg caucgaaaau gugagacccu acuccccuuc ucagcaggac     2400

cuucuccggc uguaugagga aggggcuugg aacagccccg agauugauga gaaacagguu     2460

gacgaaaugc uggccauccu caagaaguuu aaugagaccg auuccagcaa acgcccgaca     2520

aagucugaaa uuaaaagaua ccggcugugg cuugagcaaa aguaucgcuc cccuuacacg     2580

ggcaccccca ucccucugag caaacucuuc acaaccgcgu augaaauuga gcacgucauc     2640

cccagaucuc gguuuuucga cgauucccug acaaacaagg ugauuuguga gagcgaagug     2700

aauaagcuga aaggaaacau gcuuggcuac gaguuuauca agucucacua uggggaaaaa     2760

guccagcuca gcuccggaag cgaggugcag aucuucucug uuaaugacua cgagaaguuu     2820

gugaaagacc auuacuccaa gaacaaggcu aaaaugaaga aacugcugau ggaugacauu     2880

ccugaaaagu ucaccgagcg ccagaugaac gauagcagau auaucucccg gcucauuguc     2940

ucucugcuua gcaauaucgu gagagaaaaa ggcgagcaag aauccauuuc uaagaacguu     3000

cuggugugca ccgggggagu cacggaccgc cucaagaaug auuggggcgu gaacgacguu     3060

uggaauaaaa ucauccugcc ccgguuuaag agaaugaacg agauuacaga uagcaccgac     3120

uacacauccg ugaauaccuc ugggcacauc auuccggcca ugccucugca ucuucagaaa     3180

ggcuucaaca agaaacgcau cgaucaccgg caccaugcca uggacgcuau ugucaucgcc     3240

ugugcuggaa gaaauaucgu gaacuaucug aauaaccaga gcuccaagag cgaugugucu     3300

cgccacgacc uccggaaucu gcucugcgag aaggacaaau ccgauagaaa ggaacugauu     3360

aucaaacccu gggacacguu uacacaggau gucuacagcg uucuuucuuc cauuaucgug     3420

agcauuaagc aaaaccugcg cgugaucaau aaaaccaaga acauuuauca gcggaaagag     3480

gacggcaagg ucaucaucuc uaagcaggaa aacaauauua acuggugggc caucagaaaa     3540

ccccuccaua aggagacagu guccggggaa guuaaucugc ggaaagagac caccauuagc     3600

cugaaggagg cgaucgauaa cccugcccgc auuaucgaca aagauuucaa gaagaaacuu     3660

agagaacuga uccucauggg auacaaugag aagcugauua aaaaguauuu cuacgacaac     3720

aaugaauccu ggcaagaugu gaaccucaaa aagaucccgg cuuacuauuu uacaaaggac     3780

acgaaugauc gguacuucgc cagccgcucu cugcuugacg agaacuuuga uaaaaagaaa     3840

auugaaaaua aggucaccga cacaggcauc agaaccauuc ugcuccggca ccuggaggug     3900

gaagggggag acagcaaagu ugcuuucucc cccgaaggcc uggagagaau gaacaagaau     3960

aucgaaaucc uuaacggggg caagaaacau aagccuauuu auaaagugcg cgaguacgag     4020

aaggccgaaa aauuugcggu cggaaugcgg ggcaauaagg ccucuaaauu cguggaggcu     4080

gccaagggga caaaccuguu uuucgcuguu uaugauaaug gaaagaaaag auccuuugcc     4140

accgugcccc ucaacgaagu cauccgccug cagaaggagg gccucccuuc cgcccccgcu     4200

aacgaagagg ggcugcuucc uaaauucgug cugucuccca augaccuggu guacgucccg     4260

acggaggaug aacuccggag cggacugcuu ccugagaacc ccgcgccuga aagaauuuau     4320

aagaugguuu ccugcacagg cuaccagugc uuuuucaucc acuucucugu gagcggggug     4380

cugugugaca aaaaggaguu uuccagccuc aauaagaugg aacgcgccau uucuggcgag     4440

augaucaaag agauuuguau cccccugaag gucaaccggc ucggaaaaau cauguacaau     4500

cuguga                                                                4506


<210>  4573
<211>  4506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4573
augaagacca uccugggccu cgaccugggg acaaacagca uuggaugggc caaaaucucc       60

auugaugaca auggcaacua ccuuagagau auccagcugg ggucucggau uauccccaug      120

agccaagacg ugcucggaaa guucgauaau ggcgucacgg agucccagac cgcuguucgc      180

acaggguuua gaggacugcg gcgcaucaga gaacggucuc uucugcgcag agagcggcuc      240

caccgcgugc ugcaugugau gggcuuccug ccuccgcacu augacagcgc cauuggcugg      300

aacaaagagg aacccuccac cuacgggaag uuucucaauc auggagaacc uaaacuggcg      360

ugggagagac agggcgaugg gucuaugcgg uuccuuuuua uggacagcuu ccacaagaug      420

auggaggauu uugcucccca ucagccugcc cuggucgccg acggacgcaa gaucccccuc      480

gauuggacac uguauuaccu uagaaagaag gcucugaccc aaccuauuaa aaaggaggaa      540

cucgccugga uccugcugaa cuucaaucag aaacggggcu auuaccagcu ccgcggggaa      600

gaggaggaag agaacccgac aaagagagaa gaguaucacg aacugaaggu gguuuccgug      660

gaggcugccg aggaaggaaa aggcggcaau accugguaca acgucaaacu ugagaauggg      720

uggauuuacc accggcccag caaaaucgac cugucugauu gggugggaaa gacgaaacag      780

uuuauuguua ccauggaaua ugaggcggac ggcaucacac ucaagaaaaa caaggaaggg      840

gaggugaaga gauccuuccg cgucccugac gaggaugacu ggggacugcg gaaaaagaga      900

accgaaagcc aacuugaggc cucuggcaaa acaguggggg cuuuuaucua cgaucaucug      960

cucgccgaac ccgcugacaa gauucgcgga aauuucaucc ggaccauuga gagaaaauau     1020

uacaagcagg aacuggccga uauccugcgc gagcagucca aguaucacga ggaacuccag     1080

agcaaaaaga uucuggcgga cugcgccgag gaacuuuacc ggucuaacca accucauaga     1140

gauucccugc ucaaaaagga caugguguau cugcuucuga cggaucucau cuuuuaccag     1200

cgcccccuga aaagcaagaa gucccugauc gcuaauuguc cguacgagua uuacgaguau     1260

gucgacaaag aaacaggcga gauuaagcgg cagggcauca aaugcguugc caagucuaac     1320

ccuuacuauc aagaguucag acucuggcag uucgugcuga accuucgccu guacgaucgg     1380

accgaagaca aagaagugac agcugaguau cuccccagca aggaggacua cgccagacug     1440

uuugucuacc ugaaugaucg gaaggaaauu aaccaggaga cccuucucaa agacuucuuu     1500

aagcugaaaa aggugaaaca gcgcuccgaa aaggaguucc cuguuagaug gaauuauauc     1560

gaagauaaag agaagaagua ccccuguaac gagacccggc acgaacugcu cauggcccug     1620

gacagagcug ggauggagcg cagcuggcuu gaugacacag cgcggcaaua uagacugugg     1680

caccuccugu acucuauuga agugaaagau gaggccgaac gcgcucugag aaaacuuaag     1740

gacgaugacg aguuugucuc cgccuuccuc aaggugaaac ccuuuaagaa ggauuaugga     1800

gccuacagcg agaaagcugu uaagaaacug cuggccguga ugcggauggg cucucauugg     1860

ugcgaagacg auaucuguaa ggagacgaaa gaacgcaucg agcacauucu ccagggggac     1920

gucgaggaaa agaucaagga gagaaugaau cauaccgcgu auagccuggg agaacuuucc     1980

gacuuccagg gccugccuga guggcucgcu ugcuacgugg ucuacgggcg gcacucugaa     2040

gccacagaua uuaaacauug gaagaccccg gagagccuga uggccuauau caacggcuuu     2100

aaacagcacu cccugcgcaa ucccauugug gagcaaugua uccuugaaac acucagaacc     2160

guucacgaca uuuggaagga ggcuggacau aucgaugaaa uccacgugga gcugggccgg     2220

agcaugaaau cuacguccga ccagcgcgcc agaaugacag aaaacauucu gcggaaugag     2280

aacaccaauc uccgcaucaa gagccugcuu auggagcuga agaacgaugc ugacauuaaa     2340

gaugucagac cuuacucucc caugcagcag gaaauccucc ggauuuauga ggaaggggcc     2400

cugcaagagc ugaccaagga agacaaugau uacgaggaga ucuucaaaau cuccaagauu     2460

ccucagccca gcgcgaaaga acuucucaag uauaaacugu ggcuggagca gaaguacaga     2520

uccccguaua caggaaagcc uaucucucuc agcaaacugu uuaccuccgc cuaccagauu     2580

gaacauauca uuccccaauc ucgcuacuuc gacgauagcu uuaacaauaa ggugaucugc     2640

gaguccgagg uuaacgcucu uaaaagcaac augcugggcu augaguucau caagaaucac     2700

gggggagaaa uugugcacug uacacuccug ggcgacguca aagugcagaa cgaaucugag     2760

uacaaggccu ucgugacgga acauuaugcu aauaacaagu acaaaaagga gaaacugcuu     2820

auggaggaua ucccucagga cuuucugaau cggcaccuca acgacuccag auauauuagc     2880

aagguuguca aaacccugcu cucuaauauc gugcgcacag aagaugagau ggaaaccaca     2940

uccaaguucg uggucagcug caccgggggc auuacggacc ggcugaagaa agauugggga     3000

cuuaacgacg uguggaauag caucguuuac agcagauuug agcgccugaa cgaacucacc     3060

aaguccgaug ccuucggccg gugggagaau aaagagggga agagaguguu ucaaacaagc     3120

gucccccugg aacugcagcg cggauucaac aaaaagcgga ucgaccauag acaccaugcg     3180

auggaugccc uugugauugc uugugccucu cggaauauca uuaacuaucu gaauaacgag     3240

uccgcuaaca gcccuaagaa acgcgaagac cucagacagc agcugugcga uaagaaucgg     3300

aucauucgca aacccuggga guccuuuacc caagacgcca gagccgcucu cgaagauguu     3360

guggucucuu ucaagaacua cgugcggguu aucaauaaaa caaccaacua cuaugagaga     3420

uacggcgcgg acggggagaa gacgauugcc aagcagaaaa auagcgaaau gugggcugug     3480

cgcaagccga ugcacgaggc caaauauuac ggacggguca accugcagag aaagacagaa     3540

gugcgccuua aagccgcucu ggaggacauc ccuuccaucu gcaauaagga gcuccgggau     3600

uauauuaaca gacuggugaa aucuggccug aaugcccgcc agcuucuggc gcacuuuaag     3660

agcaccgacu acuguuggaa caagcaaaau gucgauaaag uugaagugug gcgguucucc     3720

gacgagaagg aaccccucgu ggcuaucaga gagucucugg auacaucuuu ugacgccaaa     3780

cgcauugaua ccaucaccga cacagggauu cagaagaucc uccugaacua ucuugaagcc     3840

aaaggcggag aucccgcuau cgccuucacg ccugagggca uugcggagau gaaucagaac     3900

auggcucugu acaauaacgg gaagaugcau cagccgaucc ugagaguccg gcucuccaag     3960

aaacgcggaa agaaauacca agugggccag accgggugca aggccaaaca guuuguuauu     4020

agcgaaucug gaacaaaccu guauuucgcc aucuacgagg acgaagauag aaaucggacc     4080

uauaacacaa uuccccuuaa ugagguggcu gaacgccuga agcaaggccu cuccccuguc     4140

cccgagaaga acgagaaaga agugccucug aaguucuacc ucagccccaa ugaccugguu     4200

uaugugccua ccgaggaaga gauccuuaug aaagaaugua agcugaacaa aaauagaauc     4260

uacaaguuua uugauucuuc cgggacgaca gccaacuucg ucccccaccg gagcgcuucu     4320

cugaucuaua auguggacaa gaaacucgcc gaggcguuuu gcaacgacga gauuauccag     4380

aaugaauacg gcguuggauc cccgcagagc aagaaccaga aagcccugac cggcgagaug     4440

auuaaggcug uguguuggaa acuugaaguc gauagacugg ggaauaucau ucgcgugauc     4500

aaguga                                                                4506


<210>  4574
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4574
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

ggcaauauca acgaugacgg guccgagcag cuggugaaaa uucaggcuuc uggaagcaga      120

aucauuccca uggaugccgc gaugaucggc gacuucaaua aggggaacuc caucucucaa      180

acagcugaac ggacgcgccu uagagggauu cggcgccuga gcgagagaua ccuccugcgg      240

cgcgaaagac uucaccggau ccuggauauu cucggcuuuc ugccuuccca uuucgcccgc      300

gaccuggaua gauauggcaa aaucgugaag gacaaagagc cgaagcucgc cuggcggaag      360

aaugaagcug ggcaguuuga guucauuuuu cagaacucuu ucaaagagau gcuggaagau      420

uuuaagcuga aucaccccaa ccugaucacc gacgauaaaa aggucccuua cgacuggaca      480

auuuauuacc uccgcaaaaa gggacugacc agcaagaucu ccaaagagga acuugccugg      540

auccugcuca auuucaacca aaagagaggc uauuaccagc ugcggggaga ggaggaagag      600

gaaaauaaaa acaaacaggu ggaguuuuau gcucugaaag uuauugccgu ggaagauagc      660

ggagagaaga aaggcaagga cacaugguac aauguccauc ucgagaacgg guggaucuac      720

cgcagaaccu cuaacaugcc ccuggacugg gugggaaaga caaaagaguu cauuguuacc      780

acggaucuug aaaaggacgg caccccuaaa aaggauaaag aaggcaauau caagcggucc      840

uuuagagcgc ccaaggagga cgauuggagc cuggugaaaa agaaaacaga agccgacauu      900

gaucagaccc acaagacagu cgggaccuau aucuacgaca cgcuccuggg aaacccuucu      960

caaaagaucc gcggccggcu ugugagaaca auugagcgca aguauuacaa ggaggaacug     1020

aaacucaucc uggauaagca gaaagaguuc cauccggaac ugcaggaccg ggcucucuau     1080

gccgcuugcg uggaggaacu guacaccauu aaugaggccc acagaaacaa uaucgggaac     1140

cgcgauuuua cauaucuuuu ccuggaggac auucucuucu accagcggcc ccugaagucc     1200

aaaaagagcc uuaucgacaa uuguccuuac gaagagaacc acugccugga uaaggaaacc     1260

ggagagauca aauuuuuccc cgucaagugu auugcgaaau cucauccucu cuuucaagag     1320

uucagacugu ggcaguuuau cguuaaucug cgcauuuauc ggaagggccu cgcccaggac     1380

guggauguga ccaacgaacu gcuuaaaaca gaggaagacu acgucgcucu guucgauugg     1440

cucaauggga agaaagagau cgaccaaaag gccuuucuga aguauccccc guucggacug     1500

aaaaaggaag uggagaacua cagauggaau uauguucagg auaaauccua cccuugcaac     1560

gagacgcgca gccuuaugcu cgcucggcug gaaaagugug acauuuccau cgaguuucug     1620

accaaagaaa augaggaagc ccucuggcac auucuguauu cuguggagga uaagaacgag     1680

aucgucaagg cccuucagaa auucgcugac aagcauaacc ugaaugcgag cuuuguggau     1740

guuuuccaga aauuucccuc cuucaagaaa gaauacggca gcuacucugc caaggcuauc     1800

aagaaacucc ugccucugau gagaaugggc aaguauuggu ccgaggacag cauugauaaa     1860

gggacacggg aacgcaugga caagaucauu accggagagu acgacgaaaa gaucgagaac     1920

agagugcggg agaaggccau ucaccuugcc gauaucucug aguucaaggg ccucccccug     1980

uggcuggucu gcuauaucgu guacgggaga cacagcgaau ccaaagaaau ugcuaagugg     2040

gagucucccg ccgacaucga uagcuaucuc aaauccuuua agcaacauag ccugcgcaau     2100

ccuauugucg agcagaucau uaucgaaaca cuucgggugg uuagagacau cuggaaacag     2160

gugggaaaca uugaugagau ccacgucgaa cugggccgcg agaugaagaa uccggcggac     2220

aaaagacgga agaugaccca gcaaauuucu gaaaacgaga auacgaaccu ccgcaucaag     2280

uaccugcuga cagaguuccu uaaucccgaa uaugaggugg aaaacguuag accuuacucc     2340

cccagccagc aggauauucu ccggaucuac gaggaagggg ugcuuaauuc ugucuccaac     2400

cugccugagg agaucagcga cauucucaaa aaguuuaccg aaaccgaucu gaaaaagcgc     2460

cccuccagau cugaggugcu ucgguauaaa cuguggcucg aacagaagua ccgcagcccg     2520

uauacaggcg aggugauccc ucugggaaag cuguucaccc ccgcuuacga aauugagcau     2580

guuaucccuc aauccagaua uuuugacgau ucuuucagca auaaagucau uugugaggcc     2640

gaagugaaca aacuuaaauc caaucuccug ggccacgagu uuaucgugaa gcaucacggg     2700

gaaauugucg agcugcccuu cgggaaaaag gugaagaucu uuacaguuga cgaguacgaa     2760

caguucguga aagauaacua uagccggacg cgcggcaaga ugaaaaagcu ccuggucgac     2820

gacaucccug aggaauuugu ggccagacag cuuaacgauu cucgguacau uuccaaaguu     2880

gucaagagcc ugcucucuaa uaucgugaga gagaaggggg aagaggaggc uauuuccaaa     2940

aacgugaucc ccugcaccgg aggcguuaca gaccgccuga agaaagauug gggagugaau     3000

gacgucugga acaagauuau ccugccgcgg uuccagagac uuaaugaacu gaccggcaca     3060

aacaaauuua ccacgaugag ccucaauaac cacgagaucc cugccaugcc ccuggaacuc     3120

caaaagggau ucaauaagaa acgcauugau caucggcacc augcuaugga cgccaucauu     3180

aucgcgugug ccaacagaaa uauuaucaac uaccugaaua acgagucugc uaccgccaag     3240

gcugaacuua gccgcuauga ucugcagcgg cuccugugcg acaaagccaa gacagaugac     3300

aauggcaacu acaaaugggu gaucagaaag cccugggcgu ccuucaccca ggaugugcac     3360

gggguccugg aggacauuau cguuagcuuu aaacaaaacc uucgcgugau uaauaaggcc     3420

acaaaccacu uccugcauua uaaugaggaa ggaaagaaaa ucuuugugaa gcaggagaaa     3480

ggcgacucuu gggcuauucg gaaguccaug cacaaagaua ccgucuucgg ggaagugaac     3540

cucagacgga ucaagacggu ugcccugaau gaggcuauga agaacccuca gagcauugug     3600

gucaaagacu uuaagcgcaa acuccuggaa cuucugaauc ucggauucga ugccaagaga     3660

aucaaaaagu acuuugagga caaccgggag acaugguccg auaucaaucu gucuaagauu     3720

accguguauu acuucagcaa agacacaaag gaucgcuuuu ucgccaccag aaaaccgcug     3780

gacaccuccu uugaucggaa gaaaaucgaa aacaauauua cagacacggg cauccagaag     3840

auucuucuga gacaucucga gcugaaggau aacaaucccg acaucgcuuu cucuccugau     3900

gggaucgacg aaaugaaccg caauauuauc caacucaaca auggcaaaua ucaccagccc     3960

auuaucaagg uucgguggua cgagcaggcg gacaaauucg ccgugggaca gaccggcaac     4020

aagagcucca aauuugucga ggcugccaag gggacaaacc uguucuuugc cguguacgaa     4080

ucuaauauuc uugaugagaa aaccaacaca aucaucaaga agagaaauua ugcuaccauu     4140

ccucugaacg uugccaucga acgccaaaaa cagggacuga gcguggcgcc cgaggaagag     4200

aauggcaacg acccuauuuu cguccucucc cccaaugauc ugguguaccu uccgacgaca     4260

gacgagcugg cuaacggggu ggucgcccag ccucucgaua aggaacggau cuauaaaaug     4320

guuagcugua ccggaaauga gggccauuuu auucccgcca gaaucgcuaa cccuauucug     4380

cagaccaucg aacucggguc uaauaacaag gcccaaaaag cguggacaga cgagaugauc     4440

aaggaaauuu gcaugcccau caagguggau cgccugggca auauucuuga guccagcucu     4500

cugcacaaaa aguga                                                      4515


<210>  4575
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4575
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

ggcuccguga augaugacgg gucugagcag cuggugaaaa uccaagcuag cggauccaga      120

auuaucccca uggaugccgc gaugauuggc gacuucaaca agggaaauuc uaucagccag      180

acagcugaac ggacgcgccu uagaggaguc cggcgccugu ccgagagaua ccuccugcgg      240

cgcgaaagac uucaccggau ccuggauauu cucggcuuuc ugccuuucca uuuugcccag      300

gaccuggauc gccacggcaa aaucgugaag gggaaagagc cgaagcucgc cuggagaaag      360

aacgaagcug gacaguucga guuuauuuuc caagacucuu uuaaagagau gcuggaagau      420

uucaaacuua auuaucccaa ccugaucacc gacgauaaaa agguuccuua cgacuggaca      480

auuuauuacc uccggaaaaa gggccugacc agcaagaucu ccaaagagga acuugccugg      540

auucugcuca auuuuaacca gaagcgcggg uauuaccagc ugagaggaga ggaggaagag      600

gaaaauaaag auaagcuggu ggaguucuau gcucucaaag ucguggccgu ugaagacagc      660

ggcgagaaga aagggaagga caucugguac aacgugcauc uggagaaugg augggucuac      720

cggcgcacau cuaacauguc ccuugauugg gugggcaaga ccaaagaauu uaucgugaca      780

accgaccugg agaaggaugg cacgcccaaa aaggacaaag aagggaaugu caagcggagc      840

uucagagcgc cuaaggagga ugacuggucu cucguuaaaa agaaaaccga agccgauauu      900

gaccagacac acaagaccgu gggagcuuau aucuacgaua cacugcuugg caaccccucc      960

caaaagauua gagggcgccu ggugcggacc aucgagagaa aguauuacaa ggaggaacuc     1020

aaacugauuc uggagaagca gaaagaauuu caucucgagc ugcaggaccg cgcccuuuau     1080

gcugccugca ucgaggaacu guacccuauc aacgaggcgc accggaauaa cgucggaaau     1140

agagauuuca cguaucucuu ccuggaagac auucuguuuu accagcgccc gcuuaagagc     1200

aaaaagucuc ucaucgacaa cugucccuac gaggaaaauc aaggccugga uaaggagaca     1260

ggggagauua aauccuuccc ugugaagugc aucgccaaaa gccacccccu guuucaggag     1320

uuccggcucu ggcaguuuau uaucaaccug agaaucuauc gcaaggaccu ugcucaagau     1380

guugacguga ccaaugaacu gcucaaaaca gaagaggauu acgucgcccu guucgacugg     1440

cugaacggaa agaaagaaau ugaucagaag gcuuuucuua aguauccugu guucggccuc     1500

aaaaaggaga ucgagaauua ccgguggaac uauguucagg acaaacccua cccguguaau     1560

gaaaccagau cccugaugcu ggcccgccuc gagaagugcg auauuucuau cgaauuucug     1620

accaaagaga acgaagaggc ccuuuggcau auucuguaua gcguggagga caagaaugaa     1680

aucgucaagg cucuccagaa auucgcggau aagcacgacc ugaacgccuc cuuuguggau     1740

gucuuccaaa aauuuccucc cuucaagaaa gaguacggca gcuacucugc uaaggccauu     1800

aagaaacugc uuccucucau gcggaugggg aaguauuggu ccgaaaauag caucgacaaa     1860

ggaacaagag agcggaucga caagauuauc acgggcgaau acgaugagaa aauugagaac     1920

cgcgugagag aaaaggccau ccaucuggcu gacguuucug auuucaaggg gcugccccuc     1980

uggcuggugu guuauauugu cuacggacgg cacagcgagg ccaaagaaau cauccagugg     2040

aagucccccg cggacauuga uucuuaucuu cgcagcuuua aacagcacuc ccugagaaac     2100

ccuaucgugg agcagauuau cauugagacc cuccggguug ugagagacau cuggaagcaa     2160

gucggcaaua ucgaugaaau ucauguggag cuggggcgcg aaaugaaaaa cccggcugac     2220

aagagaaaga agaugacaca gcagaugagc gagaaugaaa acaccaaucu gcggaucaag     2280

uaccuucuca cagaguuccu gaaccccgag uaugaagugg agaauguucg cccuuacucu     2340

cccucccagc aagauauucu gagaaucuac gaagagggcg uccucaacag cgugucugac     2400

cugccugaag agauuuccga uauccuuaaa aaguuuaccg agacggaccu gaaaaagcgg     2460

cccagccgcu ccgaagugcu cagauauaaa cuguggcugg agcagaagua ccggucuccg     2520

uauacaggag aagucauccc ucuuggcaaa cucuucaccc ccgccuacga gauugaacac     2580

gugaucccuc agagcugcua uuuugaugac uccuucucua auaaaguuau uugugaggcc     2640

gaggugaaca agcugaaaag caaucugcuc gggcaugaau uuaucgucaa gcaccaugga     2700

gagauugugg aacugcccuu cggcaaaaag guucgcaucu uuaccgugga cgaguacgag     2760

caguucguca aggauaacua uuccagaaca cggagcaaaa ugaagaaacu ucugauggac     2820

gauauuccug aagaguuugu gacccgccaa cucaaugacu cuagauacau cuccaagguu     2880

gugaaaagcc ugcugucuaa caucguccgg gaaaaggggg aggaagaggc uauuuccaag     2940

aaugugaucc ccugcacagg aggcgugacg gauagacuua aaaaggacug ggggauuaac     3000

gaggucugga acaaaaucau ucugccgcgc uuccagcggc ucaaugaacu gaccggcaca     3060

aacaaguuua ccaccaaaaa uguuggaauc caggagaucc cuacaaugcc ccucgaacug     3120

caaaagggcu ucaacaagaa aagaauugau caccgccacc augccaugga cgcuaucauu     3180

aucgccugug cgaaucggaa cauuaucaau uaccuuaaca augagagcgc cacggcuaag     3240

gccgaacugu cuagauauga ucuccagaaa augcugugcg acaaggcuaa aaccgauaac     3300

aaugggaacu acaagugggu gauccgcaaa cccugggcca gcuucacaca ggacaccuau     3360

cuggcgcuug agaauauuau cguguccuuu aagcagaacc ugcgggucau uaauaaggcc     3420

acaaacagau uccuccacua caacgaggaa gggaaaaaga ucuuugugaa acaaggcaag     3480

ggggacaauu gggcuauucg caaaagcaug cauaaggaua ccguuuucgg agaggugaac     3540

cugcggagaa ucaagacggu cgcccucaau gaagcuauga aaaacccuca gucuauugug     3600

guuaaggacu uuaaacggaa gcugcuugag cuguggaauc ucggcuucga ugccaaacgc     3660

aucaagaagu auuuugaaga caacagagag acaugguccg auaucaaucu gagcaaaauu     3720

gagguguacu auuucuccaa ggacaccaaa gaucgguuuu ucgccacacg caagccgcug     3780

gacaccucuu uugaucggaa aaagaucgaa aacaauauua ccgacacagg gauccagaag     3840

auucuucuga gacaccucga gcugaaagau aacaaucccg acaucgcuuu cagcccugau     3900

ggcaucgacg aaaugaacag aaauauuauc cagcucaaca auggaaagua ccaccaaccc     3960

auuaucaaaa uucgcuggua cgagcaggcg gacaaguucg ccgucggcca gacggggaac     4020

aaauccucua aguuugugga ggcugccaaa ggaaccaauc uguucuuugc cguuuaugaa     4080

agcaacaucc uugauaagaa gacaaacacc aucauuaaaa agcggaauua cgcuacaauc     4140

ccucugaacg uggccauuga gagacagaaa caaggccugc ccgucgcgcc ugaagacgag     4200

aaugggaacg aucccaucuu cgugcucucc ccgaaugacc ugguguaucu uccuaccgau     4260

gacgaacugg cuaacggaau uaucgcccag ccccucgauc gcggccggau uuacaagaug     4320

gucucuagcu ccggggagca gugcuuuuuc aucaaacaua ucguugccaa ugugcuggug     4380

gacaaguuug aguucagccc ucucaacaag auggaaagag cucugacggg cgagaugauu     4440

aaaaugaucu guauucccau caaggucgau cgccuuggaa augugcugga aucuuccagc     4500

ucuuauaaaa aguga                                                      4515


<210>  4576
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4576
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

ggcuccguga augaugacgg gucugagcag cuggugaaaa uccaagcuag cggauccaga      120

auuaucccca uggaugccgc gaugauuggc gacuucaaca agggaaauuc uaucagccag      180

acagcugaac ggacgcgccu uagaggaguc cggcgccugu ccgagagaua ccuccugcgg      240

cgcgaaagac uucaccggau ccuggauauu cucggcuuuc ugccuuucca uuuugcccag      300

gaccuggauc gccacggcaa aaucgugaag gggaaagagc cgaagcucgc cuggagaaag      360

aacgaagcug gacaguucga guuuauuuuc caagacucuu uuaaagagau gcuggaagau      420

uucaaacuua aucaucccaa ccugaucacc gacgauaaaa agguuccuua ugacuggaca      480

auuuacuauc uccggaaaaa gggccugacc agcaagaucu ccaaagagga acuugccugg      540

auucugcuca auuuuaacca gaagcgcggg uacuaucagc ugagaggaga ggaggaagag      600

gaaaauaaaa acaagcuggu ggaguucuac gcucucaaag ucguggccgu ugaagauagc      660

ggcgagaaga aagggaagga caucugguau aaugugcacc uggagaacgg augggucuac      720

cggcgcacau cuaauaugcc ccuugacugg gugggcaaga ccaaagaauu uaucgugaca      780

accgaucugg agaaggacgg cacgccuaaa aaggauaaag aagggaacgu caagagaucc      840

uuccgggcgc ccaaggagga cgauuggagc cucguuaaaa agaaaaccga agccgacauu      900

gaucagacac auaagaccgu gggagcuuac aucuaugaca cacugcuugg caacccuucu      960

caaaagauua gagggcgccu ggugcggacc aucgagagaa aguacuauaa ggaggaacuc     1020

aaacagauuc uggagaagca gaaagaauuu caccuggagc uccaggaucg cgcccuguac     1080

gcugccugca ucgaggaacu uuauccgauc aaugaggcgc accggaacaa ugucggaaac     1140

agagacuuca cguaccuguu ccucgaagau auucuguuuu aucaacgccc ccuuaagucc     1200

aaaaagagcc ugaucgacaa uuguccuuac gaggaaaacc agggccucga caaggagaca     1260

ggggagauua aaucuuuccc cgugaagugc aucgccaaau cccauccucu guuucaggag     1320

uuccggcugu ggcaauuuau uaucaaucuc agaaucuacc gcaaggaucu ggcucaggac     1380

guugauguga ccaacgaacu ucugaaaaca gaagaggacu augucgcccu cuucgauugg     1440

cugaauggaa agaaagaaau ugaccagaag gcuuuucuga aguaccccgu guuccggcuu     1500

aaaaaggaga ucgagaacua uagauggaau uacguucagg auaaaccgua uccuuguaac     1560

gaaacccgca gccucaugcu ggcccggcug gagaagugcg acauuuccau cgaauuucuc     1620

accaaagaga augaagaggc ccuguggcac auucuuuacu cuguggagga uaagaacgaa     1680

aucgucaagg cucugcaaaa auucgcggac aagcaugauc ucaaugccag cuuuguggac     1740

gucuuccaga aauuuccccc uuucaagaaa gaguauggcu ccuacagcgc uaaggccauu     1800

aagaaacugc ugccccuuau gagaaugggc aaguacuggu cugaaaacuc caucgauaaa     1860

gggacacggg cccgcaucga caagauuauc acgggagagu augacgaaaa gauugagaac     1920

agagugcggg agaaggcuau ccaccucgcc gauguuagcg acuucaaggg ccugccccug     1980

uggcucgugu guuacauugu cuaugggaga cacucugaag cgaaagagau cauccagugg     2040

aaaagcccug cugauauuga cuccuaccug cgcucuuuua aacagcauag ccuucggaau     2100

ccgaucgugg aacaaauuau cauugagacc cugagaguug ugcgcgauau cuggaagcag     2160

gucggaaaca ucgacgagau ucacguggaa cucggcagag agaugaaaaa ucccgccgau     2220

aagcggaaaa agaugacaca gcagaugucc gaaaacgaga auaccaaccu gcgcaucaag     2280

uaucugcuua cagaguuccu caauccugaa uacgaggugg aaaacguuag acccuauagc     2340

ccuucucaac aggacauucu gcggaucuac gaggaagggg uccucaauuc cgugagcgau     2400

cugcccgagg aaauuucuga cauccugaaa aaguuuaccg agacggaucu uaaaaagcgc     2460

ccguccagaa gcgagauccu gcgguacaaa cucuggcugg aacagaagua ucgcuccccu     2520

uacacaggcg aggugauucc ccugggaaaa cuuuucaccc cugccuauga aaucgagcau     2580

gucauucccc agucuugcua cuuugacgau agcuucucca acaaagugau cugugaagcu     2640

gagguuaaua agcucaaauc uaaccugcug ggccacgagu uuauugugaa gcaucacggg     2700

gaaaucgucg agcucccuuu cgggaaaaag gugagaauuu uuaccguuga cgaauaugag     2760

caauucguga aggacaauua cagccggaca cgcuccaaaa ugaagaaacu gcuuauggau     2820

gacauccccg aggaauuugu caccagacag cugaacgaua gccgguauau cucuaaggug     2880

guuaaauccc uccugagcaa uauugugaga gagaagggcg aagaggaagc caucucuaag     2940

aacgucauuc cgugcacagg gggagugacg gaccgccuga aaaaggauug gggcaucaac     3000

gaggugugga auaaaauuau ccuuccucgg uuccagagac ugaacgagcu caccgggaca     3060

aauaaguuua ccacaaagaa cgucggccug caagaaaucc ccaccaugcc ccucgagcug     3120

cagaagggau ucaauaagaa acgcauugac caccggcauc acgcuaugga ugccaucauu     3180

aucgcgugug ccaacagaaa uauuaucaac uaccuuaaua acgaauccgc uacggccaag     3240

gcugagcuga gccgcuacga ccuccagaaa augcugugcg auaaggccaa aaccgacaau     3300

aacggcaauu auaagugggu uauccggaaa ccuugggcgu cuuucacaca ggauaccuac     3360

cuggcccuug aaaacauuau cgugagcuuu aagcaaaauc ugagagugau uaacaaggcu     3420

acaaaccgcu uccuccauua uaaugaggag gggaaaaaga ucuuugucaa acagggaaag     3480

ggcgacaacu gggccauucg gaaauccaug cacaaggaca ccguguucgg ggaaguuaau     3540

cugagacgga ucaagacggu ggcucucaac gaggccauga aaaauccgca gagcauuguc     3600

gugaaggauu uuaaacgcaa gcugcuugaa cuguggaacc ucggauucga cgccaaaaga     3660

aucaagaagu acuuugagga uaaucgggaa acauggucug acaucaaccu guccaaaauu     3720

gagguuuauu acuucagcaa ggauaccaaa gaccgcuuuu ucgcuacaag aaagccccug     3780

gauaccuccu uugaccggaa aaagaucgag aauaacauua ccgauacagg cauccagaag     3840

auucuucuga gacaccucga acugaaagac aauaacccug auaucgcguu cucucccgac     3900

gggaucgaug agaugaaucg caacauuauc caacucaaua acggcaagua ucaucagccu     3960

auuaucaaag ugcgguggua cgaacaggcc gacaaguucg cugucggaca gacgggcaau     4020

aaauuuagca aguucgugga ggccgccaaa gggaccaacc uguuuuucgc uguuuacgag     4080

uccaacauuc uugacaagaa gacaaauacc aucaucaaaa agagaaacua ugccacaauu     4140

ccccugaaug uggcgaucga acgccaaaaa cagggacugc cugucgcucc cgaggaugaa     4200

aacggcaaug acccgauuuu ugugcucucu ccuaacgauc ugguguaccu ucccaccgac     4260

gaugagcugg ccaaugggau cauugcccag ccucucgacc ggggaagaau cuauaagaug     4320

gucagcuccu cuggcgaaca auguuucuuu auuaaacaca ucguugcuaa cgugcuggug     4380

gauaaguucg aguuuagccc ccucaauaag auggagcgcg cccugacggg ggaaaugauc     4440

aaaaugauuu gcaucccgau uaaggucgac cggcuuggca acgugcugga guccucuagc     4500

ucccauaaaa aguga                                                      4515


<210>  4577
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4577
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuaucgag       60

aaugauuuca accggaaaga aggcaagauu gaggcucugg ggucccgcau cauucccaug      120

ucucaggaca uccucggaaa auuugauagc ggcgugacag ucucccaaac ggccgcgaga      180

accaauuacc gggggauccg cagacuguau cagcgggaca accuucgcag agaacggcug      240

caccgcguuc ucaauauucu gggauuccug ccuaagcauu acgcugaguc uaucgauuuu      300

gaagacagac ucggccaguu caaaccggag acagagguga agcugcccua ucggaaggau      360

agcgacgaua acuaccacuu uauuuuccag gaauccuuua augagauggu ggaagaguuc      420

caauuuaaaa acccuucucu uuucuauacc aaggccaaug gcgagcagag caaaaucccc      480

uacgacugga cacuguauua ccuccgcaag aaagcccugu ccaagaagau uagcaaagag      540

gaacuugcuu ggauccugcu caacuuuaau cagaagagag gguacuauca gcugcgggga      600

gaagaggaag ccgauucuaa agacaagcug gaggaguacc augcucucaa agucuccgau      660

gugcaagcca ccgaagacac aaacgcgaag ggcaccuggu auaauguuau ucuggagaac      720

ggguggaucu accgcagaca gagcaaagac ucucuuuucg auuggaaggg aaagacgcgg      780

gaauuuaucg ugaccacauc ccuggagaaa ggcggggccg ucaagauuga caaagaagga      840

aaugagaagc gcagcuucag agcuguggau ucugagaaag acuggaucgc cauuaagaag      900

aaaaccgaaa acgaucucgc uaacgccggc acaaccguug gcguguauau cuacgagacg      960

cugcuucgga auccugacca gaagauuaga gggaaacuga ucaagacaau cgaacgcaaa     1020

uauuaccucg aggaacugaa ggcgauucug gccaagcaga uccaagagca cgcugagcuc     1080

cagaaccggu cccuguacaa agauugcauu gaggaacuuu aucagggaaa ugaagcccau     1140

aagaacaaua ucaaagagaa cggcuucgac uaccuguuuc ucgaggauau uaucuucuau     1200

caaagacccc uuaagagcaa aaaguccacc aucucuaauu guccuuacga aagccgcguc     1260

uuuauuaagg acgggaaaaa ggagauccag ccgauuaaau gcaucuccaa gagcaacccc     1320

auuuaucagg aauuucggcu guggcaguuu auccacaauc ugaaaauuua cgagaaggaa     1380

gcuaucgaug acggaaaaac agugaucaac aaggacguga ccaccgaguu ccucaauaca     1440

caagaggccu augccgcucu guuugaguuc cugaacgaua aggcggaaau uucucagaau     1500

gcccuccuga aauacuuuaa acuuuccacg aacacccaca gauggaauaa cgucgaagac     1560

aaaaaguacc cuuguaacga gacacgcagc gaguucauca aacggcuguc uaagauggau     1620

ggcauugacc ucgggaagac ccugagcccc gcuuuugaaa aaucccugug gcauaucauu     1680

uauucuguua aggauaaaaa gcaguacgug agagcccuug agacauucgc caugaaaaau     1740

ggacucgaca ucaaccaguu ucgcgaaaau uucgagaagu ucaagccuua ugauagcgcu     1800

uacggcgccu auuccgaaaa agcgcugaag aaagugcugc cccucauccg gagaggcacc     1860

uacuggaaga aagacgcuau uccggccgcc guccaagagc ggaucgugaa cauuaugaag     1920

cgcaucgaaa gcguugauuu uaaucuggag aagauugaga acuucgcuga cgaugacauc     1980

ccuaaacagc uucugaaguc uuuugccaaa gggaaggauc ccaugcaggg acucaauacg     2040

uaucaggcuu gcuacgccgu guacaacaga cacuccgaag ucagcgacau ccaacugugg     2100

aaaucuccug aggacauuac aaaguaucug gauuccuuca aacagcauag ccuucggaau     2160

cccaucgugg aacagguugu gauggagacc cucagagucg ugcgcgacau uuggcaguac     2220

uauggcaacg gggcgaagga uuucuucucc gagauccacg ucgaacuggg acgggagaug     2280

aagaaugaca aaaagaccag agaacgcauu ucuaaaagca ucacagagaa cgaaaauacc     2340

aacgagagaa ucaagaauau ucugaaagag cucaugggcg augaagcccu gcaaggggac     2400

auccggcccu acuccaaggg ccaccaggau auucuuaagc uguaugagga aggaguguuu     2460

gcuaacgccc cugagacaua caaaaacauc aagauugacg auaucgaaaa gauucgcaag     2520

aauaacucuc cgagcaaauc cgacaucguu aaguauaagc ucuggcugga gcagggcuac     2580

aucagccccu acacgggguc uccuauuccc cuguccagac uuuucaccac agauuaucaa     2640

aucgagcaua uuaucccucg gagccgcuac uuugacgauu cuaugggaaa uaaaauuauc     2700

ugugaauccg cugugaacga ggaaaagacc aauaaaacag ccuaugaguu caucaaggaa     2760

aacgcgggca gaauuaucga ccucgggcag ggaaaacagg ucccccuguu ugaccuggag     2820

gccuacgaga gccacugccg gggcuauuuc aaggggaaua ccaagaaacu caaguuucug     2880

cuuucugaag auauuccgga gggcuucauc aaccgccagc ugaaugacag cagauacauu     2940

uccaaagugg uuacgagccu ccugucuaac gugguccggg aagagggaga gcaagaagcu     3000

accuccaaga aucuggugcc ucuugugggc acaaucacca gcaaacucaa gaaggauugg     3060

gggcuggagg ccaucuggga cgaucugauu gcuccccgcu ucgaaagaau gaaccagcuc     3120

acaaaaucca ccgacuuucg gaaggagguu aucgauggac auggcaauag auauuucauu     3180

aacacggucc cugacgauau caaaaagggg uuuucuaaaa agcgcauuga ccaccggcau     3240

cacgcccugg augcccuugu gaucgcuugu acaaccaaag accacgugaa uuacauuaca     3300

agccugaaca cccagagaaa caaucgcucc cucgucucua agcugcggaa gaucgaagag     3360

aaaaccauca aggacaaaag aacaggacag cccaagacga uuaaaaucgc gaaggccuac     3420

cauaagccuu ggcccaacuu caccaaagag acauuugaaa agcugagcaa aaccgugguu     3480

uccuucaagc aaaaucagcg cauuaucaac aaaacaaaua acaagaccug gggcugggag     3540

aagguggaug gggucaugaa aaaggugcuu aaaaagcagg aaaaacggaa uugggcuauu     3600

agaaagccga uccacaagga gacguauuac ggcaaaguua agggauuuga cucuccuaaa     3660

ggcaagauca caaccgccac ccgcaaaagc cugcgggaaa uuacaaacga gagacagcuc     3720

aauaccauca cagauuccgg gauuaagaaa auccugaaca aucaucucaa gacguucauu     3780

gacgagagcg gaaagaucaa cuuugaaccc gccuucaauc aagauggcau cgaccugcuu     3840

aacaaaaaua uucagcagcu gaacgagggg aagaaucacc aacccaucua uaaagugcgg     3900

auguacaccc acaacacaau guuuucuguc ggagaugaag gcaacaaguc caccaaauau     3960

gugauuccug agagcgggac aaaucucuuc uucaacguuu acugggacga agagaauaac     4020

aagcgcaauu uugagaccgu gccgcugaac agagucaucg cuuuccagaa gcagacgucu     4080

cauauucccc ggaaagaucg caccgacgug ccuaucgaua auacacuggg cagauuucuu     4140

uucacccugu cccccgacga ucucguguau cugccuacag acgaagagag cgaaucuuuu     4200

accacauucg agauugacuc ccucaacagc aagcaggucg ccaauaucua caaauuuguu     4260

gauggauccg gcaccacggg gaacuucgug cccgcggagg cugcccgggu gaucgccaau     4320

auuaaggaca aaaaggucag aagcaacuuu ugcaagcgca auaaccuguc ugauaaacaa     4380

cuuaucaaga augaguucgg acugggcagc ccucagcuca aaaaccagaa cucccuggac     4440

gggcggcaga uuaagucuau cuguuggaaa cuggaaauua auagacuugg aaagaucauc     4500

aaggugauua aauga                                                      4515


<210>  4578
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4578
augaagaaag ugcugggccu cgacaucggg accaacagcg ucggaugggc cguugugaau       60

cagaacgaua agggcgaguu ccuguccauu gagaaaaucg ggucuagaau uauccccaug      120

agccaagacg ugauggauaa guuuggagcu ggccagacag aauccucuac ggcccagcgg      180

acccaguacc gcggggucag acggcuucgc gaaagaagcc ugcuccggcg cgagagacug      240

caccgggugc uucauauucu gcacuuccuc ccucaacauu augacgcggc uaucggaugg      300

aauaaaaagg aaaacaagac auacggcaaa uuucugccgg gcguugaggu gaauaucccc      360

ugggucgcca ccgaucaggg gcaccaguuc auuuuuaugg acuccuaugc cgagaugcug      420

aaggaucuca aacagcauca accucaccug uucgaaaagu cuagcacacc cgugccucuu      480

gacuggacca ucuacuaucu gcgcaaaaag gcucucacac aggccauuuc caaggaggaa      540

cuggcuugga uccuucugaa cuuuaaucag aaaagaggau acuaucagcu ccggggcgag      600

gagguugaag auaacaccaa gcgcgaggag uucuacgccc ugaaaguggu caagguggaa      660

cuggacaaag auaauacgag cgcgaagacc ugguacaaag ugcaccucga aaacggguuu      720

auuuaucccu gcccuucuga cacaccgauc gauuccuggg ucggaauggu uaaggacuuc      780

auugugacca caacccugga gaaggacggc agcgagaaaa gaacggccga uggggaaauc      840

aagcggucuu uucgcgcucc caaagaggac gauuggacac uucugaagaa aaagaccgaa      900

uccgagcucg aaaagagcgg aaaacaagug ggcgccuuca ucuacgacac acugcuuucu      960

aauccugauc agaagauuau cggcgggcug auuaaaacca ucgagagaaa guauuacaaa     1020

aaggagcucg aacagauucu ggagacccaa cagaaguuua ucgcugaacu guccgacaaa     1080

aagcuguucc aggccugugc ggaggaacuc uauccccuua acgauagcca ucgggagaau     1140

cugcucgaga gagacuucaa acaccuguuu guccaggaua ucauuuucua ccaucgcccu     1200

cuuaagacca aaaagucccu gaucucuaac ugcacguaug aauaccggac cuuuuacaag     1260

aauggagaga aacaaauuag ccccaucaag uguauugcca aauccaaccc ccucuuccag     1320

gaauuuagag uguggcaguu cguucagaac cugcgcaucc ugcaacggca gagaagcgag     1380

ggcgacucua ucaaguauaa uguggauguc acagcugagu uucucaaaga cgaaaacgac     1440

auugccaauc uguucaccug gcuuaacgag cgcaagguga ucaaacagga ucagcugcuc     1500

aaguccuacu uuaagcugaa aguuaauaag gaaacaaaag agcuggacua ucgguggaac     1560

uacguggaug acaagaauaa agaauauccu ugcaacgcua ccagaacgca cauucuuagc     1620

uuccucgaga aguguaagga ggaaaaacgc aucgaggaug accgguuugu cuacucucug     1680

uggcaucugc ucuauagcgu ggucgaaccc auugagcuga gaggggcccu ugccaaguuc     1740

gcugaacgcg agcaucugca cccuuccuuu guggauguuu ucgcgcaauu cccgccccuc     1800

gagaaagaau acggaucuua cagcgagaag gccaucaaac ggcugcugcc ucuuaugaga     1860

augggcgacu auuggcacga agauaagauu gacgugaaga caaaacagcg gauccagcgc     1920

aucauugaug gggaggcuga cgaugacauc acccacagau cccgggaaaa ggucgagaau     1980

cucagaagca uuaccgacuu uaaaggacug ccgcugugga aggccggcua cgugguuuau     2040

aaccgccauu cugaguccgg cgaacucaca aaauggaaga caccccacga gcugagcaau     2100

uacauccaga acgaguucaa gcaauauucu cuucggaauc cuauugugga acagaucguc     2160

cuggagacac ucagaguggu gcacgaucug uggguuaaac auggggacuu uuccgaaauc     2220

caccuggagc uuggacgcga uauuaagaac agcuccgaca aaagaaagcg gauggccgaa     2280

cgccagcaag agaaugaaaa cucuagacag cggguccucg agaugcugau cgaucugaaa     2340

aaggaggcug ccuucgcgga ccucaacccc uacagcccuu cccagcagga aaaacugcgc     2400

auuguggagg auggcgugcu uagaucuaau aucgaaauuc ccaaggacau cgagaagauc     2460

acgaaaagcg cuaagccgac cucccgggaa cugacaaaau aucgccucug gcuggaucaa     2520

aaguacagaa gcccuuacac cgggcagccc auuucucugg cccagcuuuu uacauccaaa     2580

uaugagaucg agcaugucau uccucaggcc aaguacuucg acgauagcuu uaccaacaag     2640

gugaucugcg aaucugaggu uaauaaagaa aagggaucca ugcucgcuca cgaguucauu     2700

cugaaaaacg gcgggagcac ggugaauguc ggcggacggc aagugcaggu ucugaagaac     2760

gacgccuaug uggaacucau caaucgccug uacggcaaaa acaaucccgu caaggcuaag     2820

aaacuucugg ccgaggauau cgacgaggug guuggggacu uuucugaaag acagcucaac     2880

gauacccggu auauuagcag agugguccag aagcugcugu ccaauaucgu gcgcgaggaa     2940

gacgagcaag agagcacauc uaaacacgug auuggauguu ccggccaggu caccacagcg     3000

cuuaagaaag auugggggcu gaacgacauc uggaauaaga uuauccagcc ucgguucgaa     3060

agacucaacg agaugaccgc cacggaagau uacggaguuu ggcaaagccc caaucauuuu     3120

cagauucgcg ugccggagga aauccagaag ggcuucacca aaaagcggau cgaccacaga     3180

caucacgcua uggaugcccu ggugauugcu ugcgccacac gcucccauau caacuauauu     3240

ucuaaccgga gcgggcagga cagaaaugcg aaauuugaga caacccgcac cgaucuccgg     3300

uccaagcugu guuacaaaga gaacucuagc ggcaauaacu acaauuauau cuucaacaag     3360

ccuugggaca cauucacgca agaugcccac agagcucuug aaaauauugu cgugucccac     3420

aagcagaacc ugcggguucu caccaaaaca aauaacuaca cccaguuuau caauaaggac     3480

ggcaaaaagg accugcagaa acaagugaag ggagaucauu ucgccguccg caaacccaug     3540

caccaggaga caaucuaugg gagagugucu auucggagca ucaaggaagu ugcucugaag     3600

aaagcccuug agaacuggaa gcagauugug gaccgcaccc ugaaagauaa gaucagagaa     3660

cucauuggag ccuacggcaa auaugacgcu cagacgaucc ugaaguacuu uaaggaucgg     3720

gacuauaaau ucaauaaccu caagaucaau agagucgagg uguacgagua cgaucauaac     3780

ugcgcggccg uucgcaaauc cauuggggac gaucugacaa ucaagcaaau ugaacggauc     3840

accgacgaga gcauuagaaa aauccuuaug aaccaccugu cuaauuauga ugacaaccuc     3900

aaggaggcuu uuucccccga aggacugaug gaaaugaaua agaacauuaa agagcugaau     3960

ggcgggaaga accaucagcc uaucuacaaa gugcgccuuu ucgaggaucu gggcagcaag     4020

uuuccgcucg gagucucuca caauaacccc gacaaauaug ccaucgccgc uaagggcacc     4080

aaucuguucu uugggauuua cgaagaccag gagggaaagc ggauguucga uuccaucccu     4140

cucaacauug ugaucgacag acugaaagaa aagaaaagcc ccgugccuga gacauauaau     4200

gagucuaaac uuaaguuuca ccuguccccc aacgaucugg ucuacguucc uaccgacgag     4260

gaacagaagc aacccgaacu cguggaugug gaccaucuga gcgaugagca gaaaggccgc     4320

gucuauaagg ccguguccuu cacagggaau cgggcguucu uuguuaacca cagagugagc     4380

aaggucauuc augacuacgu ggaguucgga acgaaaaaua agaucgaaaa cgaugacgau     4440

aaacgcucuc uuaagcacau cuguuggaaa cugaagguua auagacucgg cgagauuacc     4500

gacgugaagc gguga                                                      4515


<210>  4579
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4579
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

ggcuccguga augaugacgg gucugagcag cuggugaaaa uccaagcuag cggauccaga      120

auuaucccca uggaugccgc gaugauuggc gacuucaaca agggaaauuc uaucagccag      180

acagcugaac ggacgcgccu uagagggguc cggcgccugu ccgagagaua ccuccugcgg      240

cgcgaaagac uucaccggau ccuggauauu cucggcuuuc ugccuuucca uuuugcccag      300

gaccuggauc gccacggcaa aaucgugaag gggaaagagc cgaagcucgc cuggagaaag      360

aacgaagcug gacaguucga guuuauuuuc caagacucuu uuaaagagau gcuggaagau      420

uucaaacuua auuaucccaa ccugaucacc gacgauaaaa agguuccuua cgacuggaca      480

auuuauuacc uccggaaaaa gggccugacc agcaagaucu ccaaagagga acuugccugg      540

auucugcuca auuuuaacca gaagcgcggg uauuaccagc ugagaggaga ggaggaagag      600

gaaaauaaag auaagcuggu ggaguucuau gcucucaaag ucguggccgu ugaagacagc      660

ggcgagaaga aagggaagga caucugguac aacgugcauc uggagaaugg augggucuac      720

cggcgcacau cuaacauguc ccuugauugg gugggcaaga ccaaagaauu uaucgugaca      780

accgaccugg agaaggaugg cacgcccaaa aaggacaaag aagggaaugu caagcggagc      840

uucagagcgc cuaaggagga ugacuggucu cucguuaaaa agaaaaccga agccgauauu      900

gaccagacac acaagaccgu gggagcuuau aucuacgaua cacugcuugg caaccccucc      960

caaaagauua gagggcgccu ggugcggacc aucgagagaa aguauuacaa ggaggaacuc     1020

aaacugauuc uggagaagca gaaagaauuu caucucgagc ugcaggaccg cgcccuuuau     1080

gcugccugca ucgaggaacu guacccuauc aacgaggcgc accggaauaa cgucggaaau     1140

agagauuuca cguaucucuu ccuggaagac auucuguuuu accagcgccc gcuuaagagc     1200

aaaaagucuc ucaucgacaa cugucccuac gaggaaaauc aaggccugga uaaggagaca     1260

ggggagauua aauccuuccc ugugaagugc aucgccaaaa gccacccccu guuucaggag     1320

uuccggcucu ggcaguuuau uaucaaccug agaaucuauc gcaaggaccu ugcucaagau     1380

guugacguga ccaaugaacu gcucaaaaca gaagaggauu acgucgcccu guucgacugg     1440

cugaacggaa agaaagaaau ugaucagaag gcuuuucuua aguauccugu guucggccuc     1500

aaaaaggaga ucgagaauua ccgguggaac uauguucagg acaaacccua cccguguaau     1560

gaaaccagau cccugaugcu ggcccgccuc gagaagugcg auauuucuau cgaauuucug     1620

accaaagaga acgaagaggc ccuuuggcau auucuguaua gcguggagga caagaaugaa     1680

aucgucaagg cucuccagaa auucgcggau aagcacgacc ugaacgccuc cuuuguggau     1740

gucuuccaaa aauuuccucc cuucaagaaa gaguacggca gcuacucugc uaaggccauu     1800

aagaaacugc uuccucucau gcggaugggg aaguauuggu ccgaaaauag caucgacaaa     1860

ggaacaagag agcggaucga caagauuauc acgggcgaau acgaugagaa aauugagaac     1920

cgcgugagag aaaaggccau ccaucuggcu gacguuucug auuucaaggg gcugccccuc     1980

uggcuggugu guuauauugu cuacggacgg cacagcgagg ccaaagaaau cauccagugg     2040

aagucccccg cggacauuga uucuuaucuu cgcagcuuua aacagcacuc ccugagaaac     2100

ccuaucgugg agcagauuau cauugagacc cuccggguug ugagagacau cuggaagcaa     2160

gucggcaaua ucgaugaaau ucauguggag cuggggcgcg aaaugaaaaa cccggcugac     2220

aagagaaaga agaugacaca gcagaugagc gagaaugaaa acaccaaucu gcggaucaag     2280

uaccuucuca cagaguuccu gaaccccgag uaugaagugg agaauguucg cccuuacucu     2340

cccucccagc aagauauucu gagaaucuac gaagagggcg uccucaacag cgugucugac     2400

cugccugaag agauuuccga uauccuuaaa aaguuuaccg agacggaccu gaaaaagcgg     2460

cccagccgcu ccgaagugcu cagauauaaa cuguggcugg agcagaagua ccggucuccg     2520

uauacaggag aagucauccc ucuuggcaaa cucuucaccc ccgccuacga gauugaacac     2580

gugaucccuc agagcuauua cuuugaugac uccuucucua auaaaguuau uugcgaggcc     2640

gaggugaaca agcugaaaag caaucugcuc gggcaugaau uuaucgucaa gcaccaugga     2700

gagauugugg aacugcccuu cggcaaaaag guucgcaucu uuaccgugga cgaguaugag     2760

caguucguca aggauaacua cuccagaaca cggagcaaaa ugaagaaacu ucugauggac     2820

gauauuccug aagaguuugu gacccgccaa cucaaugacu cuagauacau cuccaagguu     2880

gugaaaagcc ugcugucuaa caucguccgg gaaaaggggg aggaagaggc uauuuccaag     2940

aaugugaucc ccuguacagg aggcgugacg gauagacuua aaaaggacug ggggauuaac     3000

gaggucugga acaaaaucau ucugccgcgc uuccagcggc ucaaugaacu gaccggcaca     3060

aacaaguuua ccaccaaaaa uguuggaauc caggagaucc cuacaaugcc ccucgaacug     3120

caaaagggcu ucaacaagaa aagaauugau caccgccacc augccaugga cgcuaucauu     3180

aucgccugcg cgaaucggaa cauuaucaau uaucuuaaca auaagagcgc cacggcuaaa     3240

gccgagcugu cuagauacga ucuccagaag augcugugug acaaagcuaa gaccgauaac     3300

aaugggaacu auaaaugggu gauccgcaag cccugggcca gcuucacaca ggacaccuac     3360

cuggcgcuug aaaauauuau cguguccuuu aagcagaacc ugcgggucau uaauaaagcc     3420

acaaacagau uccuccacua uaacgaggag ggaaagaaaa ucuuugugaa gcaaggcaaa     3480

ggggacaauu gggcuauucg caagagcaug cauaaggaua ccguuuucgg agaagugaac     3540

cugcggagaa ucaaaacggu cgcccucaau gaggcuauga agaacccuca gucuauugug     3600

guuaaagacu uuaagcggaa acugcuugaa cuguggaauc ucggcuucga ugccaagcgc     3660

aucaagaaau acuuugagga caacagagaa acaugguccg auaucaaucu gagcaagauu     3720

gagguguauu acuucuccaa agacaccaag gaucgguuuu ucgccacacg caaaccgcug     3780

gacaccucuu uugauagaaa gaagaucgag aacaauauua ccgacacagg gauccagaaa     3840

auucuucugc ggcaccucga acugaaggau aacaaucccg acaucgcuuu cagcccugau     3900

ggcaucgacg agaugaacag aaauauuauc cagcucaaca auggaaaaua ccaccaaccc     3960

auuaucaaga uucgcuggua ugaacaggcg gacaaauucg ccgucggcca gacggggaac     4020

aaguccucua aauuugugga ggcugccaag ggaaccaauc uguucuuugc cguuuacgag     4080

agcaacaucc uugauaagaa aacaaacacc aucauuaaga aacggaauua ugcuacaauc     4140

ccucugaacg uggccauuga aagacagaag caaggccugc ccgucgcgcc ugaggacgaa     4200

aaugggaacg aucccaucuu cgugcucucc ccgaaugacc ugguguaccu uccuaccgau     4260

gacgagcugg cuaacggaau uaucgcccag ccccucgauc gcggccggau uuauaaaaug     4320

gucucuagcu ccggggaaca gugcuuuuuc aucaagcaua ucguugccaa ugugcuggug     4380

gacaaguuug aguucagccc ucucaacaaa auggagagag cucugacggg cgaaaugauu     4440

aagaugaucu guauucccau caaagucgau cgccuuggaa augugcugga gucuuccagc     4500

ucuuacaaga aauga                                                      4515


<210>  4580
<211>  4515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4580
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

ggcuccguga augaugacgg gucugagcag cuggugaaaa uccaagcuag cggauccaga      120

auuaucccca uggaugccgc gaugauuggc gacuucaaca agggaaauuc uaucagccag      180

acagcugaac ggacgcgccu uagaggaguc cggcgccugu ccgagagaua ccuccugcgg      240

cgcgaaagac uucaccggau ccuggauauu cucggcuuuc ugccuuucca uuuugcccag      300

gaccuggauc gccacggcaa aaucgugaag gggaaagagc cgaagcucgc cuggagaaag      360

aacgaagcug gacaguucga guuuauuuuc caagacucuu uuaaagagau gcuggaagau      420

uucaaacuua aucaucccaa ccugaucacc gacgauaaaa agguuccuua ugacuggaca      480

auuuacuauc uccggaaaaa gggccugacc agcaagaucu ccaaagagga acuugccugg      540

auucugcuca auuuuaacca gaagcgcggg uacuaucagc ugagaggaga ggaggaagag      600

gaaaauaaaa acaagcuggu ggaguucuac gcucucaaag ucguggccgu ugaagauagc      660

ggcgagaaga aagggaagga caucugguau aaugugcacc uggagaacgg augggucuac      720

cggcgcacau cuaauaugcc ccuugacugg gugggcaaga ccaaagaauu uaucgugaca      780

accgaucugg agaaggacgg cacgccuaaa aaggauaaag aagggaacgu caagagaucc      840

uuccgggcgc ccaaggagga cgauuggagc cuccugaaaa agaaaaccga agccgacauu      900

gaucagacac auaagaccgu uggaguguac aucuaugaca cacuucuggg caacccuucu      960

caaaagauua gagggcgccu cgugcggacc aucgagagaa aguacuauaa ggaggaacug     1020

aaacagauuc uggagaagca gaaagaauuu caccucgagc ugcaggaucg cgcucuuuac     1080

gccgcuugca ucgaggaacu guauccgauc aaugaggccc accggaacaa ugucggaaac     1140

agagacuuca cguaccucuu ccuggaagau auucuguuuu aucaacgccc ccuuaagucc     1200

aaaaagagcc ucaucgacaa uuguccuuac gaggaaaacc agggccugga caaggagaca     1260

ggggagauua aaucuuuccc cgugaagugc aucgcgaaau cccauccucu guuucaggag     1320

uuccggcucu ggcaauuuau uguuaaucug agaaucuacc gcaaggaucu ugcccaggac     1380

guggauguca ccaacgaacu gcucaaaaca gaagaggacu auguggcucu guucgauugg     1440

cugaauggaa agaaagaaau cgaccagaag gccuuucuua aguaccccgu uuucggccuc     1500

aaaaaggaga uugagaacua ucgguggaau uacgugcagg auaaaccgua uccuuguaac     1560

gaaaccagaa gccugaugcu ggcucgccuc gagaagugcg acaucuccau ugaauuucug     1620

accaaagaga augaagaggc ccuuuggcac auccuguacu cugucgagga uaagaacgaa     1680

auugugaagg cccuccaaaa auucgcugac aagcaugauc ugaaugcgag cuuugucgac     1740

guguuccaga aauuuccccc uuucaagaaa gaguauggcu ccuacagcgc caaggcuauc     1800

aagaaacugc uuccccucau gcggaugggg aaguacuggu cugaaaacuc cauugauaaa     1860

ggaacaagag agcggaucga caagaucauu acgggcgaau augacgagaa aaucgagaac     1920

cgcguuagag aaaaggccau ucaccuggcc gaugugagcg acuucaaggg gcugccccuc     1980

uggcuggucu guuacaucgu guauggacgg cacucugagg cuaaagaaau uacccagugg     2040

aaaagcccug ccgauaucga cuccuaccuu agaucuuuua aacagcauag ccugcgcaau     2100

ccgaucguug agcaaauuau cauugagaca cuccgggugg ucagagauau cuggaaacag     2160

gugggcaaca uugacgaaau ccacguggag cuggggcgcg aaaugaaaaa ucccgcggau     2220

aagagaaaga agaugaccca gcagaugucc gagaacgaaa auacaaaccu gcggaucaag     2280

uaucuucuca ccgaguuccu gaauccugag uacgaaguug agaacguccg ccccuauagc     2340

ccuucucaac aggacauucu gagaaucuac gaagagggcg ugcucaauuc cgugagcgau     2400

cugcccgaag agauuucuga cauccuuaaa aaguuuacgg agacagaucu gaaaaagcgg     2460

ccgucccgca gcgaaauucu cagauacaaa cuguggcugg agcagaagua ucgguccccu     2520

uacaccggag aagucauccc ccuuggcaaa cucuucaccc cugcuuauga gaucgaacau     2580

gugauucccc agucuugcua cuuugacgau agcuucucca acaaaguuau cugugaggcc     2640

gaggugaaua agcugaaauc uaaccugcuc gggcacgaau uuauugucaa gcaucacgga     2700

gagaucgugg aacugccuuu cggcaaaaag guucgcauuu uuacagugga cgaguaugag     2760

caauucguca aggacaauua cagcagaacc cgguccaaaa ugaagaaacu ucugauggau     2820

gacauccccg aagaguuugu gacacgccag cucaacgaua gcagauauau uucuaagguu     2880

gugaaauccc ugcugagcaa uaucguccgg gaaaaggggg aggaagaggc caucucuaag     2940

aacgugauuc cgugcacggg aggcgugacc gacagacuua aaaaggauug ggggaucaac     3000

gaggucugga auaaaauuau ccugccucgc uuccagcggc ucaacgaacu gacaggcacc     3060

aauaaguuua caaccaaaaa cguugggauu caagagaucc ccacgaugcc ccucgaacug     3120

cagaagggcu ucaauaagaa aagaaucgac caccgccauc acgcuaugga ugccauuauc     3180

auugcuugug ccaaccggaa uaucauuaac uaccuuaaua acgaguccgc gaccgccaag     3240

gcugaacuga gcagauacga ccuccagaaa augcugugcg auaaggccaa aacagacaau     3300

aacgggaauu auaagugggu gauccgcaaa ccuugggcuu cuuucaccca ggauacauac     3360

cuggcccuug agaacaucau ugugagcuuu aagcaaaauc ugcgggucau caacaaggcg     3420

accaacagau uccuccauua uaaugaggaa ggaaagaaga uuuuugugaa acagggcaag     3480

ggggacaacu gggccauccg caaauccaug cacaaggaca cgguuuucgg agaggugaau     3540

cugcggagaa uuaagacagu cgcucucaac gaagccauga aaaauccgca gagcaucgug     3600

guuaaggauu uuaaacggaa gcugcuugag cuguggaacc ucggcuucga cgcuaaacgc     3660

auuaagaagu acuuugaaga uaauagagag accuggucug acaucaaccu guccaaaauc     3720

gagguguauu acuucagcaa ggauacaaaa gaccgguuuu ucgccacccg caagccccug     3780

gauaccuccu uugacagaaa gaagauugaa aauaacauca cagauacggg gauucagaag     3840

auccuucugc ggcaccucga gcugaaagac aauaacccug auauugccuu cucucccgac     3900

ggcaucgaug aaaugaauag aaacaucauu caacucaaua acggaaagua ucaucagccu     3960

aucauuaaaa uccgcuggua cgagcaggcu gacaaguucg cggucggcca gaccgggaau     4020

aaauuuagca aguucgugga ggccgcuaaa ggaacaaacc uguuuuucgc cguuuacgaa     4080

uccaacauuc uugacaagaa gaccaauaca aucaucaaaa agcggaacua ugccaccauu     4140

ccccugaaug uggcuaucga gagacaaaaa cagggccugc cugucgcccc cgaagaugag     4200

aacgggaaug acccgauuuu ugugcucucu ccuaacgauc ugguguaccu ucccacggac     4260

gaugaacugg cgaauggaau cauugcucag ccucucgacc gcggccggau cuauaagaug     4320

gucagcugua cagggaacga gggccacuuc auucccgcca gaaucgccaa uccgauccug     4380

caaaccauug agcucggauc caacaauaaa gcucagaagg ccuggaccga ugaaaugauc     4440

aaggagauuu gcaugccuau caaaguugac cgccugggca acgugcuuga aucuagcucc     4500

agcuacaaga aauga                                                      4515


<210>  4581
<211>  4521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4581
augaagacca uccugggccu cgaccugggg cccaacagca uuggaugggc caaaguggag       60

gucgaugacg aaggcaauua ccuuagaaac aucaagcugg ggucccggau uaucccuaug      120

acacaggaua ugcucggaga cuucgauaaa ggcguuaagg ugucucaaac ggcuauucgc      180

accgacuuua gagggaaucg gcgccugauc gagagacacc uucugcggcg cgaaagacuc      240

caucgggugc ugcacgcccu gaaauuccuc ccgaagcauu augaggaugc gaucggaugg      300

gaccgcaacg aacccgcuac auacggcaag uuugucaaua acagcgagcc uaaacuggcc      360

ugggagagac aggaagaugg cuccaugcgg uuccuuuuua uggagucuuu caaugaaaug      420

guggccgacu uugcucagaa ccagcccagc cugguuuccg augggaagaa aauuccucuc      480

gacuggacca ucuauuaccu gcgcaagaaa gcccuuacaa gacccauuaa gaaggaggag      540

cuggcuugga uccuccugaa uuucaaccaa aaacggggau auuaccagcu gcgcggcgaa      600

gaggaagagg auaaucucac caagagaaaa gaauauuaca agcugaaagu ggucggggug      660

gaggccgacg gagcgccuaa gggcgggaaa acaugguaca acauucaccu ugagaaugga      720

uggaccgccc ggugccaguc uaagaucccg cuggacgauu ggguuggcaa ggugaaagaa      780

uauauuguca cgaccgagua cgaagacgau ggcaagacac ccaaauguga caaggagggg      840

aaagugaagc gcagcuuuaa guccccugaa aaagaugacu ggcagcucau caagacccgc      900

acagagaaau ucaucgauga cagcaacaag accgugggag cuuuuauuua ugauuaccug      960

cuugagaaac ccuucgacaa gauccggggc aaguauauua gaacgaucga acgcaaauac     1020

uauaagaaug agcugcggga aauucucaga gagcaaacaa aauaccauga agggcugcac     1080

ucucuggagg cccucaagau cugcgcugag gaacuguaca aaaacaacca gcagcaccag     1140

gaguaucuuc ugaagaagga uaugaccuuu cuccugcuua augaccugau cuucuaccaa     1200

cgcccucuca aauccaagaa aagccugauu gccgacuguc ccuaugaagu cuacaaguau     1260

gaugacaaac ugacaggaaa gaaaggccag aagaagauca aaugcauugc gaagucuaac     1320

ccguacuauc aggaguuccg gcucuggcaa uuuaucgcca aucugagacu uuucuccguu     1380

accgauggga aagaggugac cgcugaauac cugaagacag aggaagacua cgugcgccuc     1440

uuuagcuucc ugaacgaucg gaaagacauu aagcaggaga cguuucugag aucuuuccuu     1500

aagcucaaaa aggaauccau cggaagcgag aaaaucuauc cuauucgcug gaauuacauc     1560

gaggaugcca acaaguccua ucccuguaau gcuacccggc augaacugcu gcucgccuuu     1620

gacagagccg auauuccucg ggagcugcuu gacgucgaag agagagaaua cagacugugg     1680

caccuccugu auucuaucga ggcuaaagag gaaacagaga gcgcgcugcg gaaacuuaag     1740

gccaacaagg cuuucggcga ucucaauauu gacgaugccu uugugaacuc cuuccugaag     1800

aucaaacccu uugaaaagga guacggcgcc uauagcgaaa aagcuauuaa gaagcugcuc     1860

gccguuauga gacgcggguc ucuguggaaa gaggcggaca ucugcgaggc uaccaagcag     1920

aauaucgcca aaauucuuca gaacgacauc gaugaaaagc ugaaaaagaa aauugaggcc     1980

gcugugucca gcuucaauaa gcucucugac uuucaaggac ugcccacaug gcuggccugu     2040

uacgucgugu acggccggca uagcgaagcu gcccagaucc agugcuggca guccccugag     2100

gaucuuaaga ccuauauuaa caaauucaag caacacucuc ucagaaaucc gaucguugag     2160

cagugucugc uggaaacgcu ccgcacagug uacgacaucu ggaaagaugc ggggcagauu     2220

gacgagaucc acauugaacu gggaagaaac augaaaagca ccgccgagca gcgggcucgc     2280

aagaccaagg auauccuuag aaacgaaaau acaaaccugc ggauuaagaa ucuccugguc     2340

gagcugaaaa acgacgauaa gaucgagaau gugcgccccu auuccccuag ccaacaggaa     2400

auccuuagaa uuuacgagga aggcgcccuc caggagcugu cuaaagaaga cgagauguuc     2460

gaggauaucu ccaagauuag caaaauggcu cagcccucuc cuaccgaacu gucccgguau     2520

aagcucuggc uggagcaaaa guaccgcagc cccuauacag ggagagucau cccgcuuucc     2580

aaacuguuua cgaccgccua ccagauugaa cauaucaucc cucagucucg guacuucgac     2640

gauagcuuua acaauaaggu gauuugcgag uccgaaguua acucucucaa agacaauaug     2700

cugggcuaug aguucaucaa ggagugugga ggccgcauug ugcacugcac acugcuuggg     2760

gacgucaaaa ucuuuaccaa ggaagaguac aaggauuucg ugacaaaaca uuauauugac     2820

aacaccuaca agagagauaa acugcuccug gaagacaucc cccaagaguu uagcgcgcgg     2880

cagcucaaug auuccagaua uauuagcaag augguucaga cggugcuguc uaacaucguc     2940

cgcgccgaga augaacagga ggcuaccucc aaauucgugg ugagcugcaa cggagccauc     3000

acaaaucggc uuaagaagga cuggggccug aacgauguuu ggucuuccau ugucuguccu     3060

agauuuaaac gccucaacga aaucaccaag acaaaugcuu ucgggcacug ggaggacaaa     3120

gccggaaagc auguguuuca aaccacggug ccccuggaac ugcagcgggg cuucagcaaa     3180

aagagaauug aucaccgcca ccaugccaug gacgcucuug ucaucgcgug cgccucucgg     3240

aacauuguga auuaccugaa caaugagagc gcuaacuccc cugagaaaag agaagaucuc     3300

cgcaagaagc ugugugacga gaaacggaag aucagaaaac ccuggaagac auuuacccag     3360

gacgcccggg aagcccucag cgauguugug gucucuuuca aaaauuaugu gcgcguuauc     3420

aacaaggcua caaauuacua cgagcacuau uccagcuccg ggaacaaggu gaugauuucu     3480

cagaaaggca augacaugug ggccaucaga aagccgcugc auaaagaaag cgucuucgga     3540

cgggugaacc uuugccgcaa gguuauugug ucccugaaaa aggcgcucga gaauaucccu     3600

gcuauuugug auaagacccu gagagacuac aucaacgccc uggucgagaa acacuuuaau     3660

acccggcaac uucuggccca cuucaagucu aucaacuaua gacucaaccg ccagugcgug     3720

gaaaaagugg aggucuggca guuuagcgau gaaaagauug agaugguugc ucggagaaaa     3780

ucccuggaca caucuuucga ugacaagcag aucagcucca uuacggauac cggcauccaa     3840

aagauucucc ugaauuaccu ucagacaaaa gacgggaacg ccgaaaucgc guuuaccccc     3900

gagggaauug aggaaaugaa uaagaacauc agccuguaua augauggcaa agcucaucag     3960

cccauccuga aggugcgcgu ggccacaccu aaagggauga aguacccggu cggacaaacc     4020

ggcaacaaag ccacgaaguu cgugguugcu gagaagggga caaaucucua uuuugccauu     4080

uacgaaaacg agcugggcgu gcggucuuac gacaccaucc cccuuaauau ugucguggag     4140

agacugaaag aaggacucuc cccuguuccc caggcuaacg aggccgauau cccucugaag     4200

uucuaucuca gccccaauga ccugguguac gucccuacca aagaaaacga ggacgaacuu     4260

ugucgcgauc ggauuuauaa guuuguggac ucuuccggca caaccgcgaa uuucguuccc     4320

cacacaagcg ccaacacgau cuacaaugug gauaaagagu augcugagaa gugcuguaac     4380

ggggccgucu uucugaauga auacggacug ggcucuccgc aguccaagaa ccagagagcu     4440

cucaccgggg agaugaucaa agaaauuugc uggaagcuga aaguggaccg ccuuggaaac     4500

aucauuaagg ugaucaaaug a                                               4521


<210>  4582
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4582
augaagaaca ucgugggccu ggaccucggg accaauagca uuggaugggc cgucguuaac       60

ggcuccguga augaugacgg gucugagcag cuggugaaaa uccaagcuag cggauccaga      120

auuaucccca uggaugccgc gaugauuggc gacuucaaca agggaaauuc uaucagccag      180

acagcugaac ggacgcgccu uagagggguc cggcgccugu ccgagagaua ccuccugcgg      240

cgcgaaagac uucaccggau ccuggauauu cucggcuuuc ugccuuucca uuuugcccag      300

gaccuggauc gccacggcaa aaucgugaag gggaaagagc cgaagcucgc cuggagaaag      360

aacgaagcug gacaguucga guuuauuuuc caagacucuu uuaaagagau gcuggaagau      420

uucaaacuua aucaucccaa ccugaucacc gacgauaaaa agguuccuua ugacuggaca      480

auuuacuauc uccggaaaaa gggccugacc agcaagaucu ccaaagagga acuugccugg      540

auucugcuca auuuuaacca gaagcgcggg uacuaucagc ugagaggaga ggaggaaaag      600

aagaaugaua acgugaugau caucaauagc aaagucauug acgugguuaa ggcugagaaa      660

gacaagaagu acgaaaaaua uugguacaac gugcugcucg agaauggccu gcuuuaccgg      720

gccgcguucu auaacgauau cucuuuuugg aaggggcaca caaaagaguu cguccugaag      780

accauuaaac ucaaguccgg aggcacaaag caggaacuga gcuuucuucc cucuuucgac      840

gagaucgaga aaauggaaaa gaaacaaaag gauaaaaugu acuucaagau uaagcugaaa      900

accgaagccg acaucgauca ggcucauaag acggugggca ccuauaucua cgacacacuc      960

cuggggaacc cuucccagaa aauucgcgga agacuggugc ggaccaucga gagaaaguau     1020

uacaaagagg aacucaagcu gauucuugau aagcagaaag aguuucaccc cgaacugcaa     1080

gaccgcgccc ucuaugcugc cugcaucgag gaacuguacc cuauuaauga ggcgcaccgg     1140

aacaaugucg gcaacagaga uuucacauac cuuuuucugg aagacauccu cuucuaucag     1200

cgcccgcuua agagcaaaaa gucucugauc gauaauuguc ccuacgagga gaaccagggg     1260

cucgacaaag aaaccggaga gauuaagucc uuuccuguua aaugcaucgc caagagccau     1320

ccccuguucc aagaauuucg gcuuuggcag uucauuguga aucugagaau cuaucgcaag     1380

gaccucgcuc aggaugugga cgucacgaac gagcugcuga aaacagagga agauuacgug     1440

gcccuuuuug acuggcucaa uggcaagaaa gagauugauc agaaggcuuu ccugaaauau     1500

ccuguuuuug ggcugaagaa ggaaaucgag aacuaccggu ggaauuaugu gcaagacaaa     1560

cccuacccgu guaacgaaac cagacuccug augcuugccc gccuggagaa gugcgauauu     1620

uccaucgagu uccucacaaa agaaaaugag gaagcccugu ggcacauccu guacucuguc     1680

gaggacaaga acgaaauugu gaaagcucug cagaaguuug cggauaagca ugaccucaau     1740

gccagcuucg uugauguguu ccggaaauuu ccucccuuca agaaagagua uggauccuac     1800

agcgcuaagg ccaucaaaaa gcugcugccu cucaugagaa ugggcaagua uuggucugag     1860

gacuccauug auaaaggcac ccgcaagcgg auggacaaaa ucauuaccgg ggaauacgac     1920

gagaagaucg aaaacagagu ccgggagaaa gugauccacc uggccgaugu cagcgacuuu     1980

cgcggacuuc cccuguggcu cguguguuau auuguuuacg gcagacacuc ugaagcuaag     2040

gagaucacac aguggaaaag ccccgccgau auugacuccu aucugaaguc uuucaagcag     2100

cauagccugc ggaacccuau cguggagcaa auuaucaucg aaacgcuuag agucgugcgc     2160

gauauuugga aacagguugg gaauaucgac gagauucacg uggaacucgg acgggagaug     2220

aagaacccgg cggauaaaag aaagaaaaug acccagcaga uguccgagaa ugaaaacaca     2280

aaucugcgca ucaaguaccu gcucaccgag uuucugaacc ccgaauacga ggucgaaaau     2340

gugagaccuu auagccccuc ucaacaggac auucuucgga ucuacgagga gggcgugcug     2400

aacuccguua gcgaucuccc ugaagagauc ucugacauuc ugaagaaauu cacagaaacc     2460

gaucugaaga aacgccccuc cagaagcgag auccuucggu auaagcucug gcuggaacag     2520

aaauaccgcu ccccguauac gggggagguc auuccucugg gcaagcucuu uacacccgcu     2580

uacgagaucg aacaugugau uccucagucu ugcuauuucg acgauagcuu uuccaauaag     2640

gugaucugug aggccgaagu caacaaacug aagucuaauc uucugggaca cgaguucauu     2700

gugaaacauc acggcgaaau cguugagcuc cccuuuggga agaaagugag aaucuucacc     2760

gucgacgagu acgaacaauu ugugaaggac aacuacagcc ggacccgcgg aaagaugaaa     2820

aagcugcuga uggaugacau uccugaggag uucguugcca gacagcuuaa ugauucccgg     2880

uauaucagca aaguggucaa gucucugcuc uccaacauug ugcgcgaaaa aggcgaggaa     2940

gaggcuauca gcaagaaugu uauucccugc acagggggag ugaccgacag acugaagaaa     3000

gauuggggca ucaacgaagu cuggaacaag aucauucucc cgcgguucca gagacugaau     3060

gagcuuacag ggacgaacaa auuuaccaca aagaaugugg gcauccaaga aauuccuacc     3120

augccccugg agcuccagaa aggauucaac aagaaacgca ucgaccaccg gcaucacgcc     3180

auggaugcua uuaucaucgc cugugcgaau agaaacauua ucaauuaccu gaacaaugag     3240

ucugccacag cuaaggccga acugucccgc uaugaccuuc agaagcugcu cugcgauaaa     3300

gcuaagaccg acaacaaugg caacuacaaa ugggugauuc ggaagcccug ggccagcuuu     3360

acgcaggaua ccuaucuggc gcucgagaau aucauugucu cuuucaaaca aaaccugaga     3420

guuaucaaua aggccacaaa ccgcuuucuu cauuacaacg aagaggggaa gaaaauuuuc     3480

gugaagcagg gaaaaggcga caauugggcu aucagaaaga gcaugcacaa agacaccgug     3540

uuuggggaag ucaaccugcg gcgcaucaag acaguggccc ucaaugaggc uaugaagaac     3600

ccucagucca uuguugugaa agauuucaag cggaaacugc uggagcuuug gaaucuggga     3660

uuugacgcca agagaaucaa aaaguauuuc gaagauaacc gggagaccug gagcgacauu     3720

aaucucucua agaucgaagu cuacuacuuu uccaaagaua cgaaggaccg cuucuucgcc     3780

acaagaaaac cgcuggauac cagcuuugac cggaagaaaa uugagaacaa uaucacagau     3840

accggcaucc agaagauucu ccugcgccac cuugaacuga aagacaacaa ucccgauauc     3900

gcuuucuccc cugacgggau ugaugagaug aacagaaaua ucauucaacu gaacaauggc     3960

aaguaucauc agcccaucau caagauucgg ugguacgagc aggcggacaa auuugccgug     4020

ggacagaccg gcaacaaguu cucuaaauuu guugaagcug ccaaggggac aaaucucuuc     4080

uuugccgugu augagagcaa cauccuggac aaaaagacga acaccauuau caagaaaaga     4140

aauuacgcua caauuccucu uaacgucgcc aucgaacgcc aaaagcaggg acugcccgug     4200

gcgccugagg augagaaugg caacgacccc auuuucguuc ucuccccgaa ugaucuggug     4260

uaucucccua ccgacgauga acuggcuaac gggaucaucg cccagccccu ugaccgggga     4320

agaauuuaca aaauggucuc uuguacaggc aaugaggggc acuuuauccc ugcccgcauu     4380

gcuaacccca uccugcaaac cauugaacug ggcagcaaua acaaggccca gaaagcgugg     4440

acggaugaga ugaucaagga aaucugcaug ccgauuaagg uggaccggcu cggaaaugug     4500

cuggaguccu cuagcuccua uaaaaaguga                                      4530


<210>  4583
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4583
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugguc ccuuaucgag       60

cacgauuuca aaaauaagca gggccagauu gaagggcugg gagugcggau cauucccaug      120

ucucaggaga uccucggcaa auuugacgcc gggcagagca ucucccagac agcugaucgc      180

acgaaguaca gaggaguccg gcgccuguau caaagagaca accuucggcg cgaaagacug      240

caucggguuc ucaaaauucu ggauuuccug ccuaagcacu acucugagag caucgacuuu      300

caggauaagg ugggccaguu caaaccgaag caggaaguga aacucaauua ucgcaagaac      360

gagaaaaaua agcaugaguu ugucuucaug aacuccuuua uugaaauggu gucugaguuc      420

aagaaugccc aacccgaacu guuuuacaac aaaggcaaug gggaggagac caagaucccu      480

uaugacugga cacuuuacua ucugagaaag aaggcgcuca cccagcagau uacaaaagag      540

gagcuggcuu ggcuuauccu gaacuucaau cagaagcggg gauacuacca acuccgcggc      600

gaagauauug acgaggauaa aaacaagaag uauaugcagc ugaaaguuaa uaaccugauc      660

gacagcgggg ccaaggugaa aggaaagguc cucuacaaug ugaucuuuga uaacggcugg      720

aaauaugaaa agcagauugu uaacaaggac gagugggagg ggagaaccaa agaauuuauc      780

auuacaacca agacgcugaa aaauggaaac aucaagcgga ccuacaaagc cguggacucc      840

gagauugauu gggcugccau caaggcuaag acagaacaag acaucaauaa agccaacaag      900

accgucggcg aguauauuua cgaaagccuu cuggauaauc ccucucagaa aauccgcggc      960

aagcucguga aaacaauuga gagaaaguuc uauaaggagg aauuugagaa acugcuuucc     1020

aagcagaucg aacugcagcc ugaacucuuc aacgaaagcc uguacaaagc gugcauuaag     1080

gagcuguacc cccggaauga gaaccaccaa ucuaauaaca aaaagcaggg guucgaauau     1140

cucuucaccg aggacaucau cuuuuaccag agaccucuga aaucccaaaa gagcaauauu     1200

ucuggauguc aguucgaaca uaagaucuau aaacagaaga acaaaaagac gggcaaacuu     1260

gagcugauua aggagccgau caagacaauu ucccgcagcc acccccucuu ucaggaguuc     1320

cggaucuggc aguggcugca aaaucuuaaa auuuacaaca aggagaaaau cgaaaauggg     1380

aagcuggagg augugaccac acagcuccug ccuaacaacg aagccuaugu cacccuguuu     1440

gacuuccuca auaccaaaaa ggagcuggag cagaagcagu uuaucgaaua cuucguuaaa     1500

aagaaacuua uugauaagaa agagaaggaa cacuuuagau ggaacuucgu ggaggacaag     1560

aaauaucccu uuuccgaaac acgcgcucaa uuccugucuc ggcucgccaa ggugaaagga     1620

aucaagaaua cggaggauuu ccugaacaaa aauacccagg ucggcagcaa ggagaacucc     1680

ccuuuuauua aaagaaucga acagcugugg cauauuaucu acagcguguc ugaccuuaag     1740

gaguacgaaa aggcucucga gaaauucgcc gaaaagcaca aucuggagaa agauuccuuu     1800

cugaagaacu ucaaaaaguu ucccccguuc guuagcgacu augcgucuua cagcaagaaa     1860

gccaucucca agcuccugcc uauuaugcgc auggggaaau auuggucuga gagcgcugug     1920

cccacacaag ucaaggaacg gucccuuagc aucauggaga gagugaaagu ucugccucuc     1980

aaggaaggau acucugacaa ggaucuggcc gaccugcuuu cccgcgugag cgaugacgau     2040

auucccaaac agcucaucaa gucuuuuauu uccuucaaag acaagaaucc ccugaaaggc     2100

cugaacaccu aucaggcuaa uuaccucguc uauggccggc auagcgagac aggggauauc     2160

cagcacugga agaccccuga ggacaucgau agauaccuga acaauuuuaa gcaacacucc     2220

cuucgcaacc cgauugugga acaggucgug auggagacgc ugcggguugu gagagacauc     2280

ugggaacauu acggaaauaa cgagaaagau uucuuuaagg aaauucacgu cgagcucggc     2340

cgggagauga aaucucccgc cgggaagcgc gaaaaacuga gccagagaaa cacagagaau     2400

gaaaacacca aucaucggau cagagaggug cugaaggaac uuaugaacga cgccuccguu     2460

gagggaggcg ugcgcgauua uucuccuagc cagcaagaga uucucaagcu guacgaagag     2520

gggaucuauc agaaucccaa caccaauuac cugaaagucg acgaagacga gauccucaag     2580

auucggaaaa agaacaaucc uacacagaaa gaaauccaga gauauaagcu guggcuugag     2640

caaggcuaca uuucccccua uaccggaaaa aucauuccgc ugacaaaacu cuucacgcac     2700

gaguaccaga ucgaacauau uaucccucag agccgcuacu augauaacuc ucugggcaau     2760

aagaucauuu gcgaguccga agugaacgag gacaaagaua auaagaccgc uuacgaguau     2820

cugaaagugg aaaaggggag caucguuuuu ggacacaaac uucucaaccu ggacgaguac     2880

gaagcgcacg ucaauaagua uuucaagaaa aacaagacaa aacugaagaa ccuccugucu     2940

gaggauauuc ccgaaggcuu caucaauaga caacuuaacg acucccggua cauuagcaaa     3000

cuggugaagg ggcuccuguc uaauaucgug cgcgagaacg gagagcagga agccaccagc     3060

aagaaucuga ucccugucac aggcgugguu accuccaaac uuaagcagga uugggggcug     3120

aacgacaaau ggaaugagau uaucgcuccc agauuuaagc ggcucaacaa acugacgaau     3180

agcaacgauu ucggcuuuug ggacaaugau auuaacgccu uccgcaucca ggugccugac     3240

ucucucauua agggauuuuc caagaaaaga aucgaucauc ggcaccaugc ccuggacgcu     3300

cuugucgugg ccuguaccag ccgcaaucac acacacuauc uguccgcgcu caacgcugaa     3360

aauaagaacu acucucugag agacaaacug guuaucaaga augagaacgg cgauuacacc     3420

aaaacauucc aaauucccug gcagggguuu accaucgaag ccaagaacaa ucuugagaaa     3480

acggugguca gcuucaagaa gaaccugcgg gugauuaaua aaacaaacaa uaaguuuugg     3540

uccuauaaag acgagaacgg aaaucucaac cugggcaagg augggaaacc gaagaagaaa     3600

cuccgcaagc agaccaaagg auacaauugg gccaucagaa agccucugca uaaagaaaca     3660

guuucuggca uuuauaacau caaugcuccc aagaacaaga uugccaccag cgugcggacc     3720

cuucugacag agaucaaaaa ugaaaagcac cucgcuaaaa ucacggaccu gcgcauuaga     3780

gagaccaucc ugcccaacca ucuuaagcac uaccugaaua acaaagggga agccaacuuc     3840

uccgaggcgu uuucucaggg cggaauugag gaucucaaua agaagaucac aacccugaac     3900

gaaggcaaaa agcaucaacc uauuuaucgg gucaaaaucu ucgagguggg gagcaaguuc     3960

uccaucagcg aagacgaaaa uucugccaaa uccaagaaau acguggaggc ugccaaggga     4020

acaaaccucu uuuucgcuau uuaucuggau gaagagaaua agaaaagaaa cuacgaaacc     4080

aucccgcuua augaggucau uacgcaccag aagcagguug ccggcuuucc caaaagcgaa     4140

cgccugucug ugcagccuga cucccaaaag gggacauucc uguuuacccu cagccccaac     4200

gaucuggugu acgucccuaa uaacgaggag cuugaaaauc gggaccuguu caaccucgga     4260

aaucugaacg uggagcagau cucuagaauu uauaaauuua ccgauuccag cgacaagaca     4320

ugcaauuuca uccccuuuca gguuuccaag cucaucuuca accugaaaaa gaaagaacag     4380

aagaaacuug acguggauuu uauuauccaa aacgaguucg gccuggggag cccucagucu     4440

aagaaucaaa agagcauuga cgaugucaug aucaaagaaa aguguauuaa acugaagauc     4500

gaccgccucg gcaacauuuc caaagcguga                                      4530


<210>  4584
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4584
augaagacca uccugggccu cgaccugggg acaaacagcg ugggaugggc cgucauuaau       60

uccguuauca aagagcaggc ugaaagaauu uggaucgaga uggccggcuc ucggauuauc      120

cccauggaug cggcugugcu uggggacuuc gauaagggaa acagcaucuc ccaaacgucu      180

gaacgcacca gacugcgggg cgugcgcaga cuccgggagc gccagcugcu uagacgggaa      240

cgccugcaca aaguccucaa uauucugggg uuucugccua accauuaccu cgaguauauc      300

gacuucgaga agcacccggg aaaauuucug agcgaugccg aacccaaacu uccuugggug      360

aaggacgagc agggcaaaua cacauuccug uuucaggccg cuuucgauga aaugcucgcc      420

gacuuugcua uucaucaacc cucccugguu ucuggcgaua agaaaauccc uuaugacugg      480

accauuuacu aucuuagaaa gaaagcccug acagagauga ucagcaagga ggaacucgcg      540

uggauucugc ugaauuucaa ccagaagcgg ggguacuauc agcuccgcgg agaggaagag      600

gaagauaaug ccggcaaauc cguggaguuu uacgcucuga agguccuuac cguggaggcc      660

acagacgaaa agaaggggag agauguuugg uacaacgugc accuggagaa uggauggauc      720

uaucggcgcu cuagcuccgu cccccucgac ugggaaggcc agaucaaaga guucauugug      780

accacggacc ugaacccuga uagcaccccg aaacuugaca aauacgggga agugaagaga      840

ucuuuucggg cucccaagga ggaugacugg augcugauca aaaagagaac agaggccgau      900

auugcgaaua cccauaaaac agucggagcc uauaucuacg acacccuccu gcaaaagccu      960

cagcagaaaa uuaucggcaa gcugguuaag acgguggaac gcaaacucua ucgggaugag     1020

cugucccuua uccugcagaa gcaaugcgcu uuccacgccg aacuccagga cagaacacug     1080

uacgcucgcu guguggaggc ccuuuauccc aacaaugaag uccagcggag aaacauugcc     1140

aaucgcgauu uuguguaccu gcucauggac gauguucugu ucuaccaacg gccucugaaa     1200

accaagaaaa gccucaucgc uaacugcccc uaugaggaga acgaauacgu ggacagagag     1260

acaggcgaaa uuaagaaggc gccgcugaaa uguaucgcca agucucaccc ucuuuuccag     1320

gaguuucgcc uguggaauuu ccucuccaac auucggaucu aucagaaaga gaaggaaauc     1380

aaugggaccc ugcaucugga cgucgaugug accgcugagu uucuuaaaac agaagacgau     1440

uacguugccc ucuucgacug gcugaacagc agaaaggaga uugaacagaa gacguuucug     1500

aaauauccca aguucggacu caaaaaggag aucuccaauu accgcuggaa cuauguggag     1560

gauaaacaau acccuugcaa ugaaacccgg cacgccauuc ugucuagacu ugagaaggcu     1620

aacgucugua ucgacuuucu gagcaaugaa aaagaggugg cccucuggca uauucuguac     1680

uccgucagcg auaagcagga aaucgagaag gcgcugcgga cauucgcugc caaaaacggc     1740

cuugacgagu cuuuugugga agccuucaag aaauuuccuc ccuucaaguc cgaguauggg     1800

gcuuacagcg ccaaagcuau uaagaagcuc cugccccuga ugcgcugcgg aaaauauugg     1860

gauguuucua gcaucgacga uaccacaaag gaaagaauca aaaagauugu ggccggcgag     1920

uacgacgaac ggaucgcggu caaagugaga gauaagguua ugaaucucuc cgacauuucu     1980

uguuuuaagc gccugccgcu uuggcuggcc ugcuauaucg uguacaaccg gcacagcgag     2040

gacaaagagg cugucaagug ggaauccccc gccgauauug acgcuuaucu cgccagcuuc     2100

aaacagcacu cucugagaaa uccuaucgug gaacagguga ucaccgaaac gcugcgcguu     2160

gucagagaua uuuggaagca aguggggcgg aucgacgaga uucauaucga gcuuggacgc     2220

gaaaugaaaa accccgcgga uaagagaaag cggaugacag cccagauucu cgagaaugaa     2280

aacgcuaacc ugcgcaucaa agcccugcuc gcugaguuca ugaauccuga auacgagauc     2340

gagaacguga gacccuacuc cccgagccag caggaaauuc ugcggaucua ugaggacaug     2400

guccuucacg ccgaagauca acugccugag gaaauugacg ccauccucaa gaaauuuaau     2460

ucuuccaagg ugcccaccaa aagcgaguuc cugcgcuaca agcuguggcu ugagcagaaa     2520

uauagauccc cuuacaccgg cgaacucauu ccccugggca agcuguuuac agcugcguau     2580

gagaucgaac auguuauccc ucagucucgg uacuucgaug acagcuuuuc caacaaggug     2640

auuugugagu cugccgucaa uaaacucaag gauaaccagc ugggguauga guucaucaaa     2700

aaucaccaug gacaaaaggu ggagguuggc uuugggaaaa gcguggagau uuucuccguc     2760

gacagcuacg aacgcuuugu gaaggagcag uacgcuaagu cugccguuaa aaugaagaaa     2820

augcuuaugg augacauccc cgaacaguuc auugagagac aacugaacga cucccgguau     2880

aucagcaagg ugauuaaagg acuccugucu aauaucguca gagagaagaa cgauuccggc     2940

gaauacgagc cggaagccgu gagcaagaau aucguggucu gcaccggguc uguuacagac     3000

cgccugaaac gggauugggg caugaacgac guguggaaua gcauugugua uccuagauuu     3060

gagcgccuua acucccugac ggauacccag agcuucggac acugggaaaa uaagucuggc     3120

aaaaaggucu uucagacaga gcucccccug gaguaccaga aaggguucuc caagaagcgg     3180

aucgaccaca gacaucacgc uauggaugcc auugugaucg cgugugcuac ccgcaaccau     3240

guuaacuauc ucagcaauga auccgccucu cggaacgcca aaauuagcag auacgaccug     3300

caacgccuuc ugugcgauaa gucccggaca gacgauaaug gaaacuauaa augggugauc     3360

aagaaacccu gggacaccuu cacgcaggac gcuagagagg uccucucuaa gaucgugguu     3420

agccugaagc agaaucugcg gauuaucaac aaaaccacaa auguguaccg ccaccuugau     3480

gccaccggca acagagucua cgaaaagcag aaaacagggg acuccugggc gauucggaag     3540

ccuaugcaca aagauaccgu guuuggagcu guuaaucugc gcaaggugaa agucgugaga     3600

cucucugugg cccuggacgc cccgacgaug aucgucgaua agcggguuaa gaacaaagug     3660

uucgagcucc ugagcuauaa guacgacaaa aagagaauug aaaaauauuu uaaggagaau     3720

gcugugcuuu ggaaggagcu ggaucucgcc cgcgucgcug uguacuauuu cacagaagcc     3780

accacagagc cccugguugc ggugcggaaa ccucuggacu ccaccuuuaa cgaaaagaaa     3840

aucaaggaga gcgucaccga uacaggcauc caaaagauuc uucugaauca ucucugcacg     3900

aaggaaggga agcccgaccu ggccuucucu gcugagggca ucgaggaaau gaacagaaau     3960

cuccugcagc uuaacaacgg aaaacgccac cagccuauuu acaaggugcg gguuuaugag     4020

cccagaggca auaaauuuuc cguggggguc cugggaaaca agggcaccaa auggguggaa     4080

gccgcuaagg ggacaaaucu guucuuugcc aucuacauua ccgaggaugg aaagcgcaca     4140

uacgagacca ucccucucaa cauggucauu gaacgggaga aacagggccu ggcccccgug     4200

ccggacaaga augaaaaagg ggauaaacuu cuguucuggc ucagcccuaa cgaccugguu     4260

uaucucccca cggaggagga acuggaguuu ggcagaauca augaaccuau cgaccgcgga     4320

agaauuuaca agaugguguc uuccagcggc aaccaauguu ucuucauccc ccagucuauu     4380

uccaaugcua ucauuccgac aaaggagcuu ggggcgaaca auaaagccga acggagcugg     4440

gauggagcua ugaucaagaa caucugcauu ccugucaagg uggaccgccu gggcagaauc     4500

auugagguua aauaucggau caaugaauga                                      4530


<210>  4585
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4585
augaagacca uccugggccu cgaccugggg acaaacagcg ugggaugggc cgucauuaau       60

uccguuauca aagagcaggc ugaaagaauu uggaucgaga uggccggcuc ucggauuauc      120

cccauggaug cggcugugcu uggggacuuc gauaagggaa acagcaucuc ccaaacgucu      180

gaacgcacca gacugcgggg cgcccgcaga cuccgggagc gccagcugcu uagacgggaa      240

cgccugcaca aagugcucaa uauucugggg uuucugccua accauuaccu cgaguauauc      300

gacuucgaga agcacccggg aaaauuucug gccgaugcug aacccaaacu ugccuggguc      360

aaggacgagc agggcaaaua cacauuccug uuucaggcug ccuucgauga aaugcucgcg      420

gacuuugcca uucaucaacc uagccuggug uccggcgaua agaaaauccc cuaugacugg      480

accauuuacu aucuuagaaa gaaagcucug acagagauga ucucuaagga ggaacucgcc      540

uggauucugc ugaauuucaa ccagaagcgg ggguacuauc agcuccgcgg agaggaagag      600

gaagauaaug cuggcaaaag cguugaguuu uacgcccuga aggugcuuac cgucgaggcg      660

acagacgaaa agaaggggag agaugugugg uacaacguuc accuggagaa uggauggauc      720

uaucggcgcu ccagcucugu gccucucgac ugggaaggcc aggucaaaga guucaucgug      780

accacggacc ugaaccccga ugggaccccu aaacuugaca aauacggaga agugaagaga      840

uccuuucggg ccccgaagga ggaugacugg augcugauua aaaagagaac agaggcugau      900

aucgccaaua cccauaaaac agucggcgcu uauauuuacg acacccuccu gcaaaagccc      960

cagcagaaaa ucauuggcaa gcugguuaag acgguggaac gcaaacucua ucgggaugag     1020

cugagccuua uccugcagaa gcaaugcgcc uuccacgccg aacuccagga cagagcucug     1080

uacgcgcgcu guguggaggc ccuuuauccu ucuaacgaag uccggagaaa gaauaucgcu     1140

aaccgcgauu uuguguaccu gcucauggac gauauucugu ucuaccagcg gccccugaag     1200

accaaaaagu cccucaucgc caauugcccu uaugaggaga acgaauacgu ugacagagag     1260

acaggggaaa uuaagaaaac accccugaag uguaucgcca aaagccaccc gcuuuuccaa     1320

gaguuucgcc uguggaacuu ccucucuaau auucggaucu aucagaagga gaaagaaauc     1380

aacggaaccc ugcaucugga cguggauguc accgcugagu uucuuaagac agaagacgau     1440

uacguggccc ucuucgacug gcucaauucc agaaaggaga uugaacagaa aacguuucug     1500

aaguauccua aauucggccu gaagaaagag aucagcaacu accgcuggaa uuauguugag     1560

gauaagcagu accccugcaa cgaaacccgg cacgcgauuc uguccucucu ugagaaagcu     1620

aaugugugua ucgacuuucu gagcaacgaa aaggaggucg cccucuggca uauucuguac     1680

uccgugagcg auaagcaaga aaucgagaaa gcccugagaa cauucgcugc caagaauggg     1740

cuugacgagu cuuuugucga ggcuuucaaa aaguuuccuc ccuucaaauc cgaguaugga     1800

gccuacagcg cgaaggccau uaagaaaaug cucccccuga ugcggugcgg caaguauugg     1860

gaugugucua gcaucgacga uaccacaaaa gaacgcauca agaaaauugu uaccggggag     1920

uacgacgaaa gaaucuccgu gaagguccgg gauaagguga ugaaccuguc ugacauuagc     1980

uguuuuagac gccucccucu guggcuugcu ugcuauaucg uuuacaaucg gcacuccgag     2040

gacaaagagg ccgugaagug ggaaagcccg gcugauauug acgccuaucu ggcgucuuuc     2100

aaacagcacu cccucagaaa ccccaucguc gaacagguga ucacggaaac acugcgcgug     2160

guuagagaua uuuggaagca ggucggacgg aucgacgaga uucauaucga gcugggccgc     2220

gaaaugaaaa auccugccga uaagagaaag cggaugaccg cucaaauucu ugagaacgaa     2280

aaugccaacc uccgcaucaa agcucugcug gccgaguuca ugaaccccga auacgagauc     2340

gagaauguga gaccuuacag ccccucucag caggaaauuc uccggaucua ugaggacacc     2400

gugcugcacg ccgaagauca gcuuccggag gaaauugacg cuauccugaa gaaauuuaac     2460

uccagcaagg ucccuacaaa auccgaguuc cuccgcuaca agcuguggcu ggagcaaaaa     2520

uauagaucuc ccuacaccgg cgaacuuauu ccucucggga agcuguuuac agcggccuau     2580

gagaucgaac augugauccc ccagagccgg uacuucgaug acuccuuuuc uaauaagguu     2640

auuugugaga gcgcugugaa caaacugaag gauaaucagc ucggauauga guucaucaaa     2700

aaccaccaug gccagaaggu cgaagugggg uuuggaaaau ccguugagau uuucagcgug     2760

gacucuuacg aacgcuuugu caaggagcaa uacgccaagu ccgccgugaa aaugaagaaa     2820

cugcuuaugg augacauccc ugaacaguuc auugagagac agcugaauga cagccgguau     2880

aucucuaagg uuauuaaagg ccuccugucc aacaucguga gagagaagaa ugauagcggg     2940

gaauacgagc ccgaagcugu cucuaagaac aucguggugu gcacgggcag cgucaccgac     3000

cgccugaaac gggauugggg aaugaaugac guuuggaacu ccauugugua uccgagauuu     3060

gagcgccuua auagccugac agauacccaa ucuuucggcc acugggaaaa caaguccggg     3120

aaaaaggugu uucagacaga gcucccucug gaguaccaga aaggauucag caagaagcgg     3180

aucgaccaca gacaucacgc cauggaugcg auugucaucg cuugugccac ccgcaaucau     3240

gugaacuauc ucuccaacga aucugccagc cggaaugcua aaauuuccag auacgaccug     3300

cagcgccuuc ugugcgauaa gaaccggacg gacgauaaug gcaacuauaa auggguuauc     3360

aagaaacccu gggacaccuu cacacaagac gccagagagg ugcucucuaa gaucgucgug     3420

agccugaagc agaaucugcg gauuaucaac aaaaccacaa auguuuaccg ccaccuugau     3480

gcgaccggga acagagugua cgaaaagcag aaaacgggag acuccugggc uauucggaag     3540

cccaugcaca aagauacagu cuuuggcgcc gugaaucugc gcaagguuaa aguggucaga     3600

cucucugugg cccuggacac cccugcuaug aucguggaua agcgggucaa gaacaaaguu     3660

uucgagcucc ugagcuauaa guacgacaaa aagagaauug aaaaauauuu uaaggagaau     3720

gccuuccuuu ggaaggagcu ggaucucgcu aaaguggccg uguacuauuu uacagaagcg     3780

accaccgagc cgcuggucgc cgugcgcaag ccccuggacu ccacauucaa cgagaaaaag     3840

aucaaagaaa gcguuacgga uaccgggauc cagaagauuc uucugaauca ucucugcaca     3900

aaggaaggca aaccugaccu ggcuuuuucu gccgagggaa ucgaggaaau gaaccggaau     3960

cuccugcaac uuaacaacgg caagucccac cagcccauuu acaaagugag agucuaugag     4020

ccucgcggga auaaguucag cgugggaguu cugggcaaca aagggaccaa guggguggaa     4080

gcugccaagg gaacaaaucu guuuuucgcc aucuacauua ccgaggaugg caaacggacg     4140

uacgagacaa ucccccucaa cgucgugauu gaaagagaga agcaggggcu ggucccugug     4200

cccgaccgca auaaaaaggg cgauaaacuu cuguuuuggc ucucuccgaa cgaccugguu     4260

uaucucccua ccgaagagga acgggaguuc ggaagaauca augagcccau cgaccgcggc     4320

agaauuuaca agaugguguc cuguaccggg aacgaaggac auuucauccc ugcuaauguc     4380

gcgaacccca uugugccggu uaucgagcug ggcagcaaua acaaagccca gcgggcuugg     4440

acaaaugaaa ugauuaagga uaucugcauc ccuauuaagg uggaccgccu ugggcacauc     4500

auugagguca aauauagaau cgaugaauga                                      4530


<210>  4586
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4586
augaagaaaa uccugggccu cgaccugggg accaacagcg ugggaugggc cgucauuaau       60

uccguuguga acgaugaggg caaggaacag cuugugaaua ucucuagcug cggguccaga      120

auuaucccca uggacgcugc cacacuggga gauuucgaca aaggcaacuc uaagagcccu      180

gucgcggagc ggacgcgccu cagaggggug cggcgccugc uugaaagauc ccugcuccgg      240

cgcgagagac ugcaccgggu ucugucugug augggauuuc ucccggaaca uuacgcugcc      300

caacuggauc gcuauggcaa auuccuuccc gagaccgagc cuaagcuggc cugguacaag      360

gacgaaacag gcaaauauag auuucucuuc caggauagcu uuaaugagau gcuggaagac      420

uuccacgagc aucaccccga gcuugucgcu gaagggaaga aaauucauua cgauuggacc      480

aucuauuacc ugcggaagaa agcccucucc cagaagauca caaaggagga acuggcuugg      540

auucugcuca acuuuaauca gaaacgcgga uauuaccaac ugagaggcga ggaagaacag      600

gaggacaaca agaaaagcgu ggaauacuau gcccuuaagg uuguggcggu cgaggauucu      660

ggggaccgga aaggaaagga uaucugguac aaugugaaac uggaaaacgg cuggguguau      720

cgcagagccu ccgggguccc ucucgacugg guuggaaagg ugaaggaguu cauugugacc      780

acagaccugg augaaaaagg caaucccaag aaagacaagg auggcaacgu caaacggagc      840

uuuagagcuc cuaaggagga cgauuggggg cuucugaaga cccgcacgca ggcccagauc      900

gacgagucug gacaaaccgu gggcacauac auuuaugaau cccuccugcg gaugccggau      960

cagaaaauca gagggaagcu gguucgcacc auugagcgga aauacuauaa ggacgaacuc     1020

agaaaaaucc ugaucaagca gagcgaguuc cacacagccc uucaggauca caaucugcuc     1080

cuggcuugua uugaagaacu uuaccccaac aaugaggcgc aucgccggcu gcucucuacc     1140

uccgacuuua cguacuuccu gguggaagau auccuguuuu aucaaagacc ucucaagagc     1200

cagaaaggac agauugacga cugccccuac gagucccacg ucuaucgcga uaagaaagac     1260

ggcucucugc aacaugugcc ucuuaagugu guuagcaaau cccacccccu guuccaggag     1320

uuccggcucu ggcacuuucu gagcuuccug agaaucuacc agcgcgagcg gaugauugau     1380

gggucucuua agcucgacgu ggaugucaca aacgaguuuc ugcagaacga agacgauuau     1440

gugaagcugu ucgacuggcu caaugagaga aaagaaaucg aucaaaagac cauccuggcc     1500

uacaaaccgu uuggacuuaa gaaaaacgag caguccaagu aucgguggaa uuacgucgaa     1560

gacaaaagcu acccuugcaa cucuacacgc gcugauauga uuuucagacu gagcaaggcc     1620

ggcgugcccg aggaguuucu cacccgggaa aaggaggaag cucuguggca uauccuguau     1680

uccauuucug acaaaaagga gcuuaccaaa gcccucggca cauucgccga aaagaauggg     1740

cugaacgaga cguuuguuga gaucuucagc aaaaugccuc ccuuugaauc cgauuacgcu     1800

gcguauagcc ugaaggccau uagaaagcuc cuggcucuua ugcgcauggg aaaauacugg     1860

gacgagcagg ccaucgacga acacacccgg ucuagaaucg agaagauucu gacaggcgaa     1920

uaugaugaga ccauccgcac aagagugcgg gagaaagcca ugcaucucac cgacauuucc     1980

agcuuccgcg ggcugccccu guggcuugcu uguuacgucg uguaugauag acacucugaa     2040

gccaaggagc ucguuaaaug ggaaacgccu gacgauaucg accauuuucu gcggaaguuc     2100

aagcagcacu cccugcgcaa uccgauugug gagcaaauca uuauggagac acucagaacc     2160

guccgggaua ucuggaaaca ggaaggaaag aucgacgaga uucacgugga acugggccgc     2220

gagaugaaaa accccgcgaa ggaaagagcu cggaucaccg cccaggugcu ugagaaugag     2280

aacacaaauc ugcgcauuaa agcccuccug gcugaguuca ugaacccuga guuugaaauc     2340

gagaauguua gacccuacag cccugcccag caagaaauuc ugcggaucua cgaggagggg     2400

guccuuuccg gcguggcuga aaaggaucuc cccgacgaua ucgccucuau ucugaagaaa     2460

uucagagaga acgacgugaa gaaacgcccg accacaagcg aaguccugcg guauaagcuc     2520

uggcuggagc agagauaccg cuccccuuau acgggacgga ucauuccccu uggcaaacug     2580

uuuaccccug cguacgaaau cgagcaugug auuccccagu cuagauauuu cgaugacagc     2640

uuuacaaaua agguuaucug cgaguccgcc gugaacaaac ucaaggacaa cugucugggg     2700

uacgaguucg ucaagaaaca cagcggagaa aagguggaac ugggcaaugg ggagaccgcu     2760

acaaucuuuu cuguugagga auaugagcgc uucgugaaag auuccuacuu uaacaauagc     2820

aagaaaauua agaaacuucu gcucgaagac aucccugaua acuucauuga gcggcagcug     2880

aaugacucua gauacaucuc caaagucguu aagagccucc ugucuaacau ugugugcgaa     2940

gagggagagc aagaaggcau gagcaaaaau gugaucgucu guaccggggg cgugacggau     3000

cgccuuaaga aagacugggg agugcaggau gucuggaacc ggaucauucu gcccagauuu     3060

cuccggcuga auaagaugac cgaccgcaca gauuucaccg ccguuuccac aaacggccau     3120

cugcuuccgg cugugccucu gcaccuccag aagggguuua auaaaaagag aaucgaccac     3180

cggcaucacg ccauggaugc gauugugauc gccugcgcug gacgcaacau ugucaauuau     3240

cugaacaaug ccagcgcuag aaaaggcucu gagaucuccc gguacgaccu ccaacaucug     3300

cuuugugaca aggugaaaac cgaugccaac gggaacuaua aguggauucu gagaaagccc     3360

ugggaaacgu ucacacagga ccucuacgcc gcucugacca auaucguugu gagcuucaaa     3420

cagaaccugc gcgucaucaa ucggacaacc aaccacuauc agcauuacaa ugaggaugga     3480

gaaaagaaaa ugauucccca aaccaagggc gacagacuug cgauccgcaa accuaugcac     3540

aaggauacag uguaugggga gguuaaccug cggaaggaga aaacgcuccc gcugaaggac     3600

guggucaaaa aucccuccgc cgugguugau aagucucuca aaaacaagcu guacgaacuu     3660

cugaaaagcc aguacgaccu gaaggcuauu accaaguauu uugagacaca ccaggaugug     3720

ugggccgacg ucaaucucaa aaagaucaaa guguacuauu ucaccaagga aacaaacgag     3780

agauuuuucg ccacccgcaa aucccuggau ucuagcuuuu cccagaagaa gauugaagag     3840

gaggugacgg acacaggcau ccaaaagauc cuucugcggc aucuccagca gaauggaaac     3900

aauccugaua uugcuuucuc ucccgacggc gucgaaagaa ugaaccagaa uaugaccauc     3960

cugaacgacg ggaaguggca ccaaccuauu uacaaaguuc gcaccuauga gaaggccgau     4020

aaauuugcgg ugggagaaaa gggcaacaag gugaaaaagu ucgucgaggc ugccaaaggg     4080

acaaaucucu uuuucgccgu guacgagagc auccaggaag acgaggcuac cggaaagcag     4140

guuagaaaac ggacauuugc cacgauuccc cugaacgaag ugaucaagcg caagaaacaa     4200

ggccuuccug cggcucccga ggauuccaag gggaaucugc cgaucuucgu ccugagcccu     4260

aacgaccucg uguaucugcc caccgaagag gagagaaauu cuggcaaaau uauccagccu     4320

cuugaucggg aacgcauuua caagaugguu uccugcacag agggagaaug ccauuguauc     4380

cccgaguacg uggccaaccc gauucugcag accaucgaac ucggcagcaa uaacaaagcc     4440

cagagagcuu ggucugggga gaugauuaag gagaucugcg ucccugugaa agucgaccgg     4500

cugggaaaug ugucccucau caagauuuga                                      4530


<210>  4587
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4587
augaagacca uccugggccu cgaccugggg acaaacagcg ugggaugggc cgucauuaau       60

uccguuauca aagagcaggc ugaaagaauu uggaucgaga cggccggcuc ucggauuauc      120

cccauggaug cggcugugcu uggggacuuc gauaagggaa acagcaucuc ccaaaccucu      180

gaacgcacaa gacugcgggg cgcccgcaga cuccgggagc gccagcugcu uagacgggaa      240

cgccugcaca aagugcucaa uauucugggg uuucugccua accauuaccu cgaguauauc      300

gacuucgaga agcacccggg aaaauuucug gccgaugcug aacccaaacu ugccuggguc      360

aaggacgagc agggcaaaua caccuuccug uuucaggcug ccuucgauga aaugcucgcg      420

gacuuugcca uucaucaacc uagccuggug uccggcgaua agaaaauccc cuaugacugg      480

acaauuuacu aucuuagaaa gaaagcucug accgagauga ucucuaagga ggaacucgcc      540

uggauucugc ugaauuucaa ccagaagcgg ggguacuauc agcuccgcgg agaggaagag      600

gaagauaaug cuggcaaaag cguugaguuu uacgcccuga aggugcuuac agucgaggcg      660

accgacgaaa agaaggggag agaugugugg uacaacguuc accuggagaa uggauggauc      720

uaucggcgcu ccagcucugu gccucucgac ugggaaggcc aggucaaaga guucaucgug      780

acgaccgacc ugaaccccga ugggacaccu aaacuugaca aauacggaga agugaagaga      840

uccuuucggg ccccgaagga ggaugacugg augcugauua aaaagagaac cgaggcugau      900

aucgccaaua cacauaaaac cgucggcgcu uauauuuacg acacgcuccu gcaaaagccc      960

cagcagaaaa ucauuggcaa gcugguuaag acaguggaac gcaaacucua ucgggaugag     1020

cugagccuua uccugcagaa gcaaugcgcc uuccacgccg aacuccagga cagagcucug     1080

uacgcgcgcu guguggaggc ccuuuauccu ucuaacgaag uccggagaaa gaauaucgcu     1140

aaccgcgauu uuguguaccu gcucauggac gauauucugu ucuaccagcg gccccugaag     1200

accaaaaagu cccucaucgc caauugcccu uaugaggaga acgaauacgu ugacagagag     1260

acaggggaaa uuaagaaagc gccccugaag uguaucgcua aaagccaccc gcuuuuccaa     1320

gaguuucgcc uguggaacuu ccucucuaau auucggaucu aucagaagga gaaagaaauc     1380

aacggaaccc ugcaucugga cguggauguc accgccgagu uucuuaagac agaagacgau     1440

uacguggccc ucuucgacug gcucaauucc agaaaggaga uugaacagaa aacguuucug     1500

aaguauccua aauucggccu gaagaaagag aucagcaacu accgcuggaa uuauguugag     1560

gauaagcagu accccugcaa cgaaacccgg cacgcuauuc uguccucucu ugagaaagcc     1620

aaugugugua ucgacuuucu gagcaacgaa aaggaggucg cccucuggca uauucuguac     1680

uccgugagcg auaagcaaga aaucgagaaa gcucugagaa cauucgccgc uaagaauggg     1740

cuugacgagu cuuuugucga agccuucaaa aaguuuccuc ccuucaaauc cgaguaugga     1800

gcguacagcg ccaaggcuau uaagaaaaug cucccccuga ugcggugcgg caaguauugg     1860

gaugugucua gcaucgacga uaccacaaaa gaacgcauca agaaaauugu uaccggggag     1920

uacgacgaaa gaaucgccgu gaagguccgg gauaagguga ugaaccuguc cgacauuucu     1980

uguuuuagac gccucccucu guggcuugcu ugcuauaucg uuuacaaucg gcacagcgag     2040

gacaaagagg ccgugaagug ggaauccccg gcggauauug acgccuaucu ggcuagcuuc     2100

aaacagcacu cucucagaaa ccccaucguc gaacagguga ucacggaaac acugcgcgug     2160

guuagagaua uuuggaagca ggucggacgg aucgacgaga uucauaucga gcugggccgc     2220

gaaaugaaaa auccugccga uaagagaaag cggaugaccg cucaaauucu ugagaacgaa     2280

aaugccaacc uccgcaucaa agcccugcug gcugaguuca ugaaccccga auacgagauc     2340

gagaauguga gaccuuacuc ccccagccag caggaaauuc uccggaucua ugaggacacc     2400

gugcugcacg cggaagauca gcuuccggag gaaauugacg ccauccugaa gaaauuuaac     2460

ucuuccaagg ucccuacaaa aagcgaguuc cuccgcuaca agcuguggcu ggagcaaaaa     2520

uauagauccc ccuacaccgg cgaacuuauu ccucucggga agcuguuuac agcugccuau     2580

gagaucgaac augugauccc ccagucucgg uacuucgaug acagcuuuuc caauaagguu     2640

auuugugagu cugccgugaa caaacugaag gauaaucagc ucggauauga guucaucaaa     2700

aaccaccaug gccagaaggu cgaggugggg uuuggaaaaa gcguugagau uuucuccgug     2760

gacagcuacg aacgcuuugu caaggagcaa uacgcuaagu cugccgugaa aaugaagaaa     2820

cugcuuaugg augacauccc ugaacaguuc auugagagac agcugaauga cucccgguau     2880

aucagcaagg uuauuaaagg ccuccugucu aacaucguga gagagaagaa ugauuccggg     2940

gaauacgagc ccgaagcggu cagcaagaac aucguggugu gcacgggcuc ugucaccgac     3000

cgccugaaac gggauugggg aaugaaugac guuuggaaca gcauugugua uccgagauuu     3060

gagcgccuua auucccugac agauacccag agcuucggcc acugggaaaa caagucuggg     3120

aaaaaggugu uucaaacaga gcucccucug gaguaccaga aaggauucuc caagaagcgg     3180

aucgaccaca gacaucacgc uauggaugcc auugucaucg ccugugcuac ccgcaaucau     3240

gugaacuauc ucagcaacga auccgccucu cggaaugcga aaauuagcag auacgaccug     3300

cagcgccuuc ugugcgauaa gaaccggacg gacgauaaug gcaacuauaa auggguuauc     3360

aagaaacccu gggacaccuu cacacaagac gcuagagagg ugcucuccaa gaucgucgug     3420

ucucugaagc agaaucugcg gauuaucaac aaaaccacaa auguuuaccg ccacuuugau     3480

gccaccguga acagagucua cgaaaagcag aaaacggggg acagcugggc cauucggaag     3540

cccaugcaca aagauacagu guucggagcu guuaaucuuc gcaaggugaa agucgugaga     3600

cuguccgugg cccucgacac cccugcuaug aucgucgaua agcggguuaa gaacaaagug     3660

uuugagcugc ucucuuauaa guacgacaaa aagagaauug aaaaauauuu caaggagaau     3720

gccuuucugu ggaaggagcu ugaucuggcg aaaguggccg ucuacuauuu cacagaagcu     3780

accaccgagc cgcucguggc cguucgcaag ccccuggaca gcacauuuaa cgagaaaaag     3840

aucaaagaau ccgugacgga uaccggcauc cagaagauuc ugcuuaauca ucugugcaca     3900

aaggaaggga aaccugaccu cgcuuucagc gccgagggca ucgaggaaau gaaccggaau     3960

cugcuccaac ugaacaacgg aaagucucac cagcccauuu acaaagucag aguguaugag     4020

ccucgcggca auaaguuuuc cguuggggug cuuggaaaca aaggcaccaa gugggucgaa     4080

gccgcuaagg ggacaaaucu guucuuugcg aucuacauua ccgaggaugg aaaacggacg     4140

uacgagacaa ucccccugaa cguggucauu gaaagagaga agcagggccu cgugccuguu     4200

cccgaccgca augaaaaagg ggauaagcug cuuuucuggc ugagcccgaa cgaccucgug     4260

uaucugccua ccgaggagga acgggaguuc ggcagaauca augaacccau cgaccgcgga     4320

agaauuuaca aaauggucuc uuguaccggc aacgaggggc auuuuauccc ugccaaugug     4380

gcuaacccca uuguuccggu gaucgaacuc ggauccaaua acaaggccca gcgggccugg     4440

acaaaugaga ugauuaaaga uaucugcauc ccuauuaagg ucgaccgccu gggccacauc     4500

auugaaguga aguauagaau cgaugaguga                                      4530


<210>  4588
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4588
augaagaaaa cccugggccu cgaccugggg acaaacagca ucggaugggc cgugauuaau       60

uccaacaucg auucugaggg caaggaaaaa cuugucggga uuagcuccug cggaucuaga      120

aucauuccca uggacgcuac gacccugggc gauuucggga agggaaauac aaaaagcccu      180

guugccgagc ggacccgccu cagaggcauc cggcgccugc uugaaagauc ccugcuccgg      240

cgcgagagac ugcaccgggu gcugucugug augggcuuuc ucccggaaca uuacgcgagc      300

cagcuggacc gcuaugggaa guuccuuccc gagacagagc cuaagcuggc uugguacaaa      360

gaugacuccg gaagauauca auuucucuuc caaaagagcu uucacgaaau gcuggaggau      420

uuccggcagc aucagcccga acuugucgcc ggcgagaaaa agaucccuua cgacuggacc      480

auuuauuacc ugcgcaaaaa ggcccucucu caggagauca caaaggagga acuggcuugg      540

auucugcuca acuuuaauca gaaaagaggg uauuaccagc ugcggggaga agaggaacag      600

gaggagaaca auaaguccgu ggaauaccac gcccuuaaag uugugagcgu cgaggauucu      660

ggcgaacgca aggggaaaga caucugguau aacgugaccc uggagaaugg auggguuuac      720

agacgggcuu ccaacauucc ccucgauugg acgggcaagg ugaaagaguu cgucgugacc      780

acagaacugg acgaugccgg caauccuaag aaggacaaag aggggaacgu gaagagaucc      840

uuucgcgcgc cgaaagaaga cgauugggga cuucugaaga cccggacaca agcccagauc      900

gacgagucug gcaaaaccgu cgggacguau auuuacgaau cccuccugug uaugcccaau      960

cagaagauca gaggaaagcu gguucgcaca aucgagcgga aauauuacaa ggaugaacuc     1020

agacagauuc uggugaaaca aagcgaguuc caugcugccc uucaggacca caaccugcuc     1080

cuguccugca ucgaggaacu uuauccuaau aacgaggcuc accgccggcu gcucucugcc     1140

agcuccuuua uuuacuuccu gaucgaagau auucuguuuu accagagacc ccucaagagc     1200

caaaaaggcc ugaucgacaa cuguccuuau gagucucaua ucuacaagga uaagaaagac     1260

gggucccuuc accaugugcc ccugaagugc gucagcaaau cucacccccu cuuccaggag     1320

uuccgccugu ggcaguuucu gagcaaucuu cggauuuauc agagagaacg caugauggau     1380

ggaucccuca agcuggacgu ggauguuacc cgggaguucc ugccuucuga agaggacuac     1440

gugaaacucu uugacuggcu gaacgaaaga aaggagauca gccaaaaguu ccuucuggcg     1500

uauaaacccu uuggccucaa gaaaaaugag caggccaacu accgguggaa uuaugucgaa     1560

gauaaguccu acccuuguaa cgagacacgc gcugaaauua aaagcagacu gucuaaggcc     1620

ggcgugcccg aggaguuccu gaccgaagag aaagaagagg cucuuuggca cauccucuac     1680

uccauuagcg acaagaagga acugaccaaa gcccugggga cauuugccgc uaagaauugc     1740

cucaacgagu cuuucguuga aguguuugcg aaaaucccgc cuuucgaguc caauuaugcc     1800

gcuuacagcc ugaaggccau ccggaaacuu cuggcccuca ugagaauggg aaaguauugg     1860

aacgagcagg cuauugaucg ccagacgcgg gacagaaucg aaaagauucu gaccggcgag     1920

uacgaugaaa caauccgcuc cagaguccgg gagaaagcca ugcugcuuac cgacauuucu     1980

agcuuucgcg ggcucccgcu guggcuggcg uguuauaucg uguacgauag acauuccgag     2040

ucuaaggaac ucgucaaaug ggagacaccc gcugacauug aucacuuccu gagcaaguuu     2100

aaacaaaauu cccuucggaa cccuaucgug gaacagguua ucaccgagag ccugcgcacg     2160

gugagagaca uuuggaagca ggaaggaaag aucgaugaga uucaugucga gcucggccgg     2220

gaaaugaaaa aucccgccaa ggagcgcgcc agaaucacag cucaggugca agaaaacgag     2280

aauaccaacc ugcggauuaa agcccugcuu gcugaauuua ugaacccuga guucgagauc     2340

gaaaauguuc accccuauuc uccggggcag caggagaucc uccgcauuua cgaagacggc     2400

gugcuguccg gaaucgccga gaaggaucug ccugacgaca uuaccgcgau ccucaaaaag     2460

uucagagaaa acgaugucaa gaaacggccc acaaccagcg aggugcugag auacaaacuu     2520

uggcuggagc agcgcuaucg gucuccuuac acaggcagag ugauuccccu cgggaagcug     2580

uucacgccug ccuaugaaau cgagcauguu aucccccaau cccgcuacuu ugacgauagc     2640

auuucuaaua aggucaucug cgaaagcgcu gugaacaaac ugaaggacaa uugucuugga     2700

uaugaguuca uuaaaaagca cuccggcgag augguggaac uggggaacgg agagaccguc     2760

ccgguguuua gcguugaaga guacgaacgg uucgugaagg agucuuauuu uggcaauucc     2820

aaaaagauga aaaagcuccu gcucgaggau aucccugaca gcuucauuga aagacagcug     2880

aacgauuccc gcuacaucuc ucgggucgug acaagccuuc uguccaaucu cguuugcgag     2940

gaaggggagc aggacggccu gucuaaaaac gugaucgucu guaccggagg cauuacagau     3000

agacugaaga aggacugggg agugcaagaa guuuggaauc gcaucauucu uccccgguuu     3060

cugagacuca acgagaucac cgggcggacg gauuucacca gcacauccgu gaauggccau     3120

cugcuccccg cccugccucu uuaccugcag aaaggguuua acaagaaacg cauugaccac     3180

agacaccaug cuauggaugc caucgucauu gcgugcgcca aucggaacau cgugaacuau     3240

cucaauaacu cuagcgcucg caagaauagc gagaucagca gauacgaccu gcagcggcug     3300

cuuugcgaaa aagugaagac cgacgccaac ggaaauuaua aguggauucu gagaaaaccg     3360

ugggagacau ucacccagga ugucuacgcu gcccucacga acaucguugu gucuuuuaag     3420

caaaaucugc gcgugauuaa ccggacaacc aauuauuacc agcacuauaa cgaagccggc     3480

gagaaaagaa ugauccccca gacaaagggg gaccgcuggg cuauucggaa accuaugcac     3540

aaggauaccg ucuacggcga agugaaucuc agaaaggaga aaacccugcc ccuucgcgac     3600

guugugaaga acccuuccau cgucguggau aaaagccuga agaauaaacu guacgagcuc     3660

cugaagucuc aguaugaccu uaaggcgauc gccaaauacu ucgaaacaca ucaagauguu     3720

ugggcugacg ugaaccugaa gaaaauuaag gucuauuacu ucacgaaaga gaccaaugaa     3780

cgguuuuucg ccacaagaaa gucccucgau cccagcuuug accagaaaaa gaucgaggag     3840

gaagugaccg auacaggagu ucagaagauu cugcuccacc aucugcagca aaacaacaau     3900

gacccugaca uggccuucuc ucccgauggc aucgaccgca ugaaccagaa uaugaccauu     3960

cuuaacgaug ggaaauggca ccagccgauc uauaagguga gaacguacga gaaagcugac     4020

aaguuugccg ucggagaauc cggcaauaaa gcgaagaagu ucguggaggc ugccaaaggg     4080

acaaaccugu uuuucgccgu guaugaaagc guccaagagg augaggcuau cggaaagcag     4140

guuuguaaac ggaccuuugc caccauuccu cugaaugaag ugaucaagcg caaaaagcag     4200

ggccuccccg cggcuccuga ggaccugaac gggaaucuuc ccaaguucgu gcuguccccg     4260

aacgaucucg ucuaccugcc uacagaagag gaaagaaaua gcucucggau uauccagccc     4320

cucgaccgcg agagaauuua caaaauggug agcuccucug gcagccaaug cuuuuucauc     4380

aagguuuucg uggccaacuc cauuugggau aaaaaugagu auucuagccu gaacaagaug     4440

gaacgggcca ucaccaauga gaugaucaaa gaaauuugug ucccuaucaa gauugacaga     4500

cuuggaaacg ugucccugau ccagauuuga                                      4530


<210>  4589
<211>  4533
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4589
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuguggau       60

uccgaggaac agcggauccu gggcaugggg ucucgcauua uccccaugga ccaaggaguc      120

cucgauacau ucagcggcgg gaauccuguu gagacgcaga ccgcugccag aacaguguac      180

cggggaaccc gcagacugcg ggaacgcgcg cuucugagac gggagcgccu cauuagagug      240

cugaacauga ugaaauuucu gccggaacac uaugcuuccc agaucgacuu cgagaagcgg      300

cucggccagu uuuucgauga gaaagaaccc aagcuggccu ggaaacaaaa ugacgagggc      360

cgcuuugaau uuaucuuuca gcagucuuuc aacgagauga uugaggaauu uaagaauaag      420

ggggucguga gagaggauaa aaagaucccu uacgacugga caauuuauua ccuucggaaa      480

aaggcccuga gccaggaaau cgagaaagag gaacucgcuu ggauucugcu uaacuucaau      540

caaaagcgcg gauauuacca gcugagaggc gaggaagagg aagagaaccc caauaagcuc      600

guugaauauu acucccugaa aaucguggau gucauugccg acgagaagcc uaacucuaaa      660

agcgauaucu gguacucccu gcaucucgag aaugggugga ucuaucggag aagcucuaag      720

auuccccugu uuggauggaa agacaagacc cgcgauuuca ucgugacaac cgaccuuaac      780

gacgauggca cgguuaaaaa ggacaaggaa gggauugaga aacgguccuu uagagcuccu      840

agcgaaaaug auugggugcu gcucaagaaa aagaccgagu cugacaucga aaaaucccac      900

aagacagucg gagccuacgu guaugagacc cugcuucaga acccgaugca gaaggugcgc      960

ggcaaacugg uccggacaau ugagagaaag uucuacaaag augaacucaa gcaaauccug     1020

gagaaacagc gcgaauuuca ucccgagcug cgggacgagg aucucuauaa ugacugcguu     1080

agagaacugu accgcagcaa cgagaccuau caguuuaucc uuucuaagcg ggauuucgug     1140

caccuguuuc ucgacgauau ucuguucuac caaagaccuc uucgcuccca aaagagcgcg     1200

aucggcgacu guauucugga auuuaaaacg uacaaggaca aagaugggaa caauaucaag     1260

gaguaucuca aagugauuuc caagucuaac ccccuguacc aggaguuccg gaucuggcag     1320

uggcuguaua aucucaagau cuacagaaaa gaagacgaug aggacgucac acugcaguuu     1380

auugccaaca ucgaagauaa ggagcagcuu uucgacuuuc ugagcaaucg gaaauccauu     1440

gaacagaagc cucuccugga guaucugauc aaagcuaagg gacuuaaaaa gcaagugaag     1500

accgaagccu accgcuggaa cuauguugag gauaaaauuu accccugcag cgagacaaag     1560

accaugaucu cuaccagacu cgcuaaagug gaaaauauuc cggacaacuu cuuuacaaag     1620

gagaucgaac agaaacugug gcauaucauu uacuccguca cggauaagaa ugaguucgaa     1680

aaggcccugc ggaccuuugc gaaaaaguau gaccucgaua ucgccagcuu cguggacaac     1740

uucaaaaaga uuccucccuu uaaaaaugag uacggcucuu ucagcgagaa ggcuaucaag     1800

aaacugcuuc cucugaugag acucgggaag uauuggaaau ggaacgauau ugacaauaac     1860

acacagaauc gcaucuccaa gaucauuacc ggagaauacg acgaggaaau caaagauauu     1920

guucgggaga agucuaucgc ccugacaaac gagaacgacu uucagggccu gcagcuuugg     1980

cucgcucaau aucuggugua cgggagacac agcgaagccg auauugccgg aaaguggcac     2040

uccgucgcug accuggagaa auaucucaau gaguucaagc agcauagccu gcgcaacccc     2100

aucguggaac agaucauuac cgaaacgcuu agagucgugc gggauaucug gcgcaaauac     2160

ggccagggcg cggagaauuu cuucucugag auucacguug aacuggggag agacaugaag     2220

aacacagccg augagcggaa aaagaucgug aaugucguga ccgaaaacga gaauaccaac     2280

cuccgcauua aagcucugcu gauggaacuu aagaauaacu ccgacggaaa gcucgagguu     2340

gagaauguga gacccuacag cccuacacaa caggauaucc ugaaaaucua ugaagaguac     2400

gccauuucua ccggccugga caacgaaaag gaugagaaag ucaaggaaga caucaaaaag     2460

auuucccggg uggcccagcc gacaacgacc gagcuccaac gcuauaagcu guggcuugag     2520

cagaaauacu guagccccua uacagggaag gugaucccuc ugggaaaacu cuuuaccgaa     2580

gaguaccaga uugaacauau cauucccaag uccagauauu acgaugacuc uuucagcaau     2640

aaaguuaucu gcgaggcugc cgucaacaag cugaaggaca aaugucuggg ccuugaguuu     2700

aucaagaauu accacgggca gauuguggaa acaggcuucg gacaaaaagu gaccaucuuu     2760

gaugaggaag cguaucagaa cuucgucaag cagcacuacg cuuauaacca uuccaaacgg     2820

acgaagcucc ugcuggagga aauuccugag aagaugaucg agcgccagau gaaugacacc     2880

agauacauuu cuaaauuugu gcucccccug cuuagcaacc ugguucgggc caaggaaaau     2940

gauaacggcg ucaauuccaa aaacgugcuc ccggugaaug ggaagaucac aaccaugcug     3000

aaacaagacu ggggacugaa cgauguuugg aaugaccuua uccugccuag auucgugcgc     3060

augaacgagc ucgccaagac aauugcuuuc accagcugga augaacagca ccagaaguau     3120

cugcccacgg ucccucucga gcugucuaaa ggcuuucaga agaaacggau cgaucauaga     3180

caccaugcca uggacgcucu ugugauugcc ugcgcgacac gcgaucacgu uaaccugcuc     3240

aauaacaagc acgccaauac cgacacaauc cgguacgauc ugcaaagaaa acugcgccuu     3300

uucgaacggg ugaccuauau ugacccccag accaagaaca acgucacaaa agacaucccu     3360

aaggaguuua agaaacccug ggauaauuuc acgguggacg cuagaaacga gcuggaaaag     3420

aucauugugu ccuuuaaaca gaaucuccgc aucauuaaca aggccaccaa uaucuacaca     3480

aaauacgaga acgggaagaa gauuaaaguc ggacaaaagg gccugaauug ggcuauccgg     3540

aaaccgcucc auaaggaaac cguuuucgcc aaagugagcc ugagaaagcg gaagacagug     3600

cgccuuucug aggcgcugaa agauuggaag aaaauugucg acaagaaacu caagcaggaa     3660

aucaagagac ugaccuguca guaugggaaa uuugaugugg acacgauccu gcgguacuuc     3720

aaggaucgca aauaucaguu uggcgagguu gacgugucca aggucaaaau guacuauuuc     3780

gaugaggaaa acgccgcugu gagaaagaau guugacacca gcuuuacaga gaaauucauu     3840

caaggaucug ugaccgauac aggcauccag aagauucuuc ugaaccaccu cgaagccaag     3900

gggaauaaag ucgagaucgc uuucagcccu gagggaauug aagagaugaa caagaauauc     3960

auccagcuga acgaaggcaa acuccaucug cccauuuuua aggugcgggu uuacgagacc     4020

aucgggaaua aauucuccgu gggagucaag ggcaacaaga aagacaagua uguggaagcc     4080

gccaaaggga caaaucuuuu cuucgcuauu uacguggaug agaacggcau cagaagcuac     4140

gagacgauuc cccugaacau cgucaucgaa cgccugaagc agggacucuc uguugugccu     4200

gagaaaaauc ggaaggaaca cucccugcug uuuuaucuua gcccgaacga ccucguguac     4260

guccccauug aggacgaaag agagaauauc cacgcgguga accuggauca acucaauaac     4320

gagcagcgca agcggauuua uaaaaugguu uccugcaccg gcucugaaug ucauuucgug     4380

ccuuacuaug ucgccagccc caucgugaau aagguugagu acuccucucu gaacaaaauu     4440

gggagaagcc uuacaggaga aaugaucaag gacgugugca uuaaacugaa ggucgaucgc     4500

cugggcaaua ucaaggggau cuccggacuc uga                                  4533


<210>  4590
<211>  4533
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4590
augaagagaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuguggau       60

uccgaggaac agcggauccu gggcaugggg ucucgcauua uccccaugga ccaaggaguc      120

cucgauacau ucagcggcgg gaauccuguu gagacgcaga ccgcugccag aacagcguac      180

cggggaaccc gcagacugcg ggaacgcgcc cuucugagac gggagcgccu cauuagagug      240

cugaacauga ugaaauuucu gccggaacac uaugcuuccc agaucgacuu cgagaagcgg      300

cucggccagu uuuucgauga gaaagaaccc aagcuggccu ggaaacaaaa ugacgagggc      360

cgcuuugaau uuaucuuuca gcagucuuuc aacgagauga uugaggaauu uaagaauaag      420

ggggugguca gagaggauaa aaagaucccu uacgacugga caauuuauua ccuucggaaa      480

aaggcucuga gccaggaaau cgagaaagag gaacucgccu ggauucugcu uaacuucaau      540

caaaagcgcg gauauuacca gcugagaggc gaggaagagg aagagaaccc caauaagcuc      600

guggaauauu acucccugaa aaucguugau gugauugcug acgagaagcc uaacucuaaa      660

ggggauaucu gguacagccu gcaucucgag aauggaugga ucuaucggag auccagcaag      720

auuccccugu uuggcuggaa agacaagacc cgcgauuuca ucgucacaac cgaccuuaac      780

gacgauggga cggugaaaaa ggacaaggaa ggaauugaga aacggucuuu uagagccccu      840

uccgaaaaug auuggguucu gcucaagaaa aagaccgaga gcgacaucga aaaaucucac      900

aagacagugg gcgcguacgu cuaugagacc cugcuucaga acccgaugca gaaggugcgc      960

ggcaaacugg ugcggacaau ugagagaaag uucuacaaag augaacucaa gcaaauccug     1020

gagaaacagc gcgaguucca ucccgaacug cgggacgagg aucucuauaa ugacugcguc     1080

agagaacugu accgcuccaa cgagaccuau caguuuaucc uuagcaagcg ggauuucguu     1140

caccuguuuc ucgacgauau ucuguucuac caaagaccuc uucgcucuca gaaguccgcc     1200

aucggggacu guauucugga auuuaaaacg uacaaggaca aagauggaaa caauaucaag     1260

gaguaucuca aagugauuag caaguccaac ccccuguacc aggaguuccg gaucuggcag     1320

uggcuguaua aucucaagau cuacagaaaa gaagacgaug aggacgugac acugcaauuu     1380

auugcuaaca ucgaagauaa ggagcagcuu uucgacuuuc ugucuaaucg gaaaagcauu     1440

gaacagaagc cucuccugga guaucugauc aaagccaagg gccuuaaaaa gcaggucaag     1500

accgaggcuu accgcuggaa cuauguggaa gauaaaauuu accccugcuc cgagacaaag     1560

accaugauca gcaccagacu cgccaaaguu gaaaauauuc cggacaacuu cuuuacaaag     1620

gagaucgaac aaaaacugug gcauaucauu uacucuguga cggauaagaa ugaguucgaa     1680

aaggcgcugc ggaccuuugc caaaaaguau gaccucgaua ucgcuuccuu cgucgacaac     1740

uucaaaaaga uuccucccuu uaaaaaugag uacgggagcu ucucugagaa ggccaucaag     1800

aaacugcuuc cucugaugag acucggaaag uauuggaaau ggaacgauau ugacaauaac     1860

acacagaauc gcaucagcaa gaucauuacc ggcgaauacg acgaggaaau caaagauauu     1920

gugcgggaga aguccaucgc ucugacaaac gagaacgacu uucaggggcu gcagcuuugg     1980

cucgcccaau aucugguuua cggaagacac ucugaagccg auauugcugg caaguggcac     2040

agcguggcgg accuggagaa auaucucaau gaguucaagc agcauucccu gcgcaacccc     2100

aucgucgaac agaucauuac cgaaacgcuu agaguggucc gggauaucug gcgcaaauac     2160

ggccaggggg ccgagaauuu cuucagcgag auucacgugg aacugggaag agacaugaag     2220

aacacagcug augagcggaa aaagaucguu aaugugguca ccgaaaacga gaauaccaac     2280

cuccgcauua aagcccugcu gauggaacuu aagaauaacu cugacggcaa gcucgaggug     2340

gagaauguua gacccuacuc cccuacacaa caggauaucc ugaaaaucua ugaagaguac     2400

gccauuagca ccgggcugga caacgaaaag gaugagaaag ugaaggaaga caucaaaaag     2460

auuucucggg ucgcucagcc gacaacgacc gagcuccaac gcuauaagcu guggcuugag     2520

cagaaauacu guucccccua uacaggaaag gugaucccuc ugggcaaacu cuuuaccgaa     2580

gaguaccaga uugaacauau cauucccaag agcagauauu acgaugacuc cuucucuaau     2640

aaagugaucu gcgaggccgc gguuaacaag cugaaggaca aaugucuggg gcuugaguuu     2700

aucaagaauu accacggcca gauugucgaa acaggauucg gccaaaaagu gaccaucuuu     2760

gaugaggagg cuuaucagaa cuucgugaag cagcacuacg ccuauaaccg gagcaaacgc     2820

acgaagcucc ugcuggaaga aauuccugag aagaugaucg agagacagau gaaugacacc     2880

cgguacauuu ccaaauuugu ccucccccug cuuucuaacc uggugagagc cgaagagaau     2940

gauaacgggg uuaauagcaa gaacgugcuc ccggucaaug gaaaaaucac aaccaugcug     3000

aagcaagacu ggggccugaa cgaugugugg aaugaccuua uccugccucg cuucguucgg     3060

augaacgaac ucgcuaaaac aauugccuuc accuccugga augagcagca ucagaaguau     3120

cugcccacgg ugccucucga acugagcaag ggguuucaga aaaagagaau cgaucaccgc     3180

caucacgcua uggacgcccu ugucauugcg ugcgccacac gggaucaugu gaaccugcuc     3240

aauaacaaac acgcuaauac cgacacaauc agauacgauc ugcaacgcaa gcugcggcuu     3300

uucgagagag uuaccuauau ugacccccag accaaaaaca acgugacaaa ggacaucccu     3360

aaagaguuua agaagcccug ggauaauuuc acggucgacg cccgcaacga acuggagaaa     3420

aucauugugu cuuuuaagca gaaucuccgg aucauuaaca aagcuaccaa uaucuacaca     3480

aaguacgaaa acggcaaaaa gauuaaggug ggacaaaaag ggcugaauug ggccaucaga     3540

aagccgcucc acaaagagac cgucuucgcg aagguuuccc ugcggaaacg caagacagug     3600

agacuuagcg aagcccugaa ggauuggaaa aagauugugg acaaaaagcu caaacaggag     3660

aucaagcggc ugaccuguca guauggcaag uuugaugucg acacgauccu gcgcuacuuc     3720

aaagauagaa aguaucaguu uggagaggug gacguuucua aagugaagau guacuauuuc     3780

gaugaagaga acgcugccgu ccggaaaaau guggacacau ccuuuaccga aaaguucauu     3840

caaggcagcg uuaccgauac agggauccag aaaauucuuc ugaaccaucu cgaggcuaag     3900

ggaaauaagg uggagaucgc cuucucuccu gaaggcauug aggaaaugaa caaaaauauc     3960

auccagcuga acgaggggaa gcuccaucug cccauuuuua aagucagagu guacgaaacc     4020

aucggaaaua aguucagcgu uggcgugaaa gggaacaaga aggacaaaua ugucgaggcc     4080

gcuaagggca caaaucuguu uuucgcgauu uacguggaug agaacggaau ccgcuccuac     4140

gaaacgauuc cccuuaacau cgugaucgag cggcugaaac agggccucag cgucguuccu     4200

gaaaagaaua gaaaagagca cucucugcug uuuuaucuuu ccccgaacga ccucguguac     4260

gugcccauug aagacgagcg cgagaauauc cacgccguca accuggauca acucaauaac     4320

gaacagcgga agagaauuua uaagauggug agcugcaccg gguccgagug ucacuucguu     4380

ccuuacuaug uggcuucucc caucgucaau aaaguggaau acagcucccu gaacaagauu     4440

ggacgcucuc uuacaggcga gaugaucaaa gacguuugca uuaagcugaa aguggaucgg     4500

cuggggaaug ucaagggaau cagcggccuc uga                                  4533


<210>  4591
<211>  4536
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4591
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cgugaucaau       60

uccgucgcug augagucugu ugaaaagaga aacauugagc uugccggcag ccggaucauu      120

cccauggacg cggcugugcu ggggaauuuc gauaacggag ugacaaaauc ccagacggcc      180

gaacgcaccu cuuacagaag cauccggcgc cucagagagc ggcaacugcu ucgcagagaa      240

cggcugcaca agguccucca gauccugggc uuucugccug agcauuaugu gaaugacauu      300

gauuucgaga accacccggg gaaaaucaag aagaauacag aacccaaacu cccuuggcgc      360

aagaacucca ugggacaguu ugaguucauu uuucaggccu cuuucgaagc uaugcugacc      420

gacuuugcca cacaucaacc cguuuucgug agcgagggca aaaaggucca cuacgauugg      480

acccuuuauu accugcggaa aaaggcucuc acagaggaaa ucuccaagga ggaacuggcc      540

uggauuaucc uuaauuuuaa ccagaaaaga ggcuauuacc agcugcgcgg ggaggaagag      600

gaggaacaga gcaauaagcu cguggaguuc uaugcgcuga gaguugugaa agucgaagcc      660

accgacgaua agaaaggaaa ggacacgugg uacaacgugc aucuggagaa uggcuggauu      720

uaccggcgca ccucuaacau cccucucgau ugggaaggga aagugaagga guuuaucguc      780

acaaccgacc ugaaugagga uggaacaccc aaacuugaca aauauggcga aguuaagaga      840

uccuuccggg cuccuaaaga ggacgauugg acccugauua agaaaaagac ggaaagcgac      900

aucgccaacu cucacaagac acucgggugc uacauuuaug augcucugcu ucaaaacccg      960

aaucagaaaa ucaagggaag acuggugcgc accguggagc ggaaauuuua caaggaggag     1020

cucagacuga uucuggagaa acagcucucc uuccaccccg aacugcagga ccgcgaucuu     1080

uaucgggccu guaucgacgc gcuguacguc agcaacgagg cccauaagaa uaacaucucu     1140

ggcagagauu ucguguaucu cuuuauugac gauaucauuu ucuaccaacg cccucugaag     1200

accaaaaagu cccuuaucga caauugcccc uacgagaaaa accaguauau ggaucgggag     1260

acaggcgagg uuaagcaggc uccuauuaaa uguaucgcca agagccaccc ccuguuucaa     1320

gaguucagac ucuggcaguu uaucuccaau auucgcaucu accagcggga aagagugaug     1380

gacgacggga agcuggaaac cgaugucgac gugacaccgg auuuccugaa aacggaggac     1440

gauuauacca cacucuuuga auggcugaac gaccgcaagg agguugauca gaaggcuuuc     1500

cuuaagaauc cucaauuugg acugaaaaag accuucacag aguaccggug gaacuaugug     1560

gaagacaaau cuuaccccug caaugagacc agaaacgcca uucucaaugg ccugcagaag     1620

gccgggguca gcgcugauuu ucugucccgg gaaguggagg aagcgcuuug gcauauccuc     1680

uauagcgucg aggacaagau ugagaucgaa ucugcccugc gcaaauucgc uuccaagcac     1740

gaucuggacg auagauuugc cgacguguuc aaaaagcucc cucccuuccg gaaagaguac     1800

ggagccuaca gcgcuaaggc cauuaagaaa cugcuucccc ugaugagaau gggcaaguau     1860

ugggaacucu cuaacaucga cgauaaaacg aaggagcgca ucgacaaaau uaucacaggg     1920

gaaugggaug agaauauuca caaccggguu agagagaagg cgaucaagcu gagcgacauu     1980

accgauuuua aaggacugcc ucuuuggcuc uccuguuacg uggucuauaa ccgccauucu     2040

gaagcuagag ccaucgccaa gugggagagc ccggaagaca ucgauuccua ccugcggcug     2100

uuccgccagc acagccucag aaaucccgug guugaacagg ugauuaccga gacacugcgg     2160

accguccgcg acaucuggaa aagaauuggc caaaucgaug aaauucaugu ggagcuuggc     2220

cgggaaauca agaacccugc ugacaaacgc gccaagauga cagcucagau cguggagaau     2280

gaaaacacga aucugagaau uaaggcccuc cuggcggagu uuaucaacuc ugaguaugaa     2340

auugagaaug uucggcccua cuccccuggg cagcaggaaa uccugcgcau uuaugaggau     2400

accguccuua acagcgugga agagaucccc gaggacaucu cugccgugcu caaaaaguuc     2460

agagaauccg aucugaaaaa gcggccgaca aaaagcgagu uucugagaua caagcucugg     2520

cuggaacaaa aauaccgcuc cccuuauacc ggagacauua ucccccuugg caagcuguuc     2580

acaucugcuu acgagauuga acacaucauu ccucagagcc gguauuuuga cgauuccuuc     2640

ucuaauaagg ucaucugcga ggccgaggug aacaaacuca agagcaauga acugggguac     2700

gaguuuauua aaaaccauca cggacaguau guugaaaucg gcuucgggaa ggugguccag     2760

aucuuuucca uugaggcuua cgagaaauuc gugaaggcca auuauagcgg caucggaucu     2820

aagaugaaaa gacugcuuau ggacgauauu cccgaaaccu uuaucgagcg ccaacucaac     2880

gacucccggu acauuagcaa gguugugaaa ucucugcugu ccaauaucgu cagagaaaag     2940

gauagcaacg gcgaguacga accugaggcg aucucuaaaa acgugauugu uuguacgggg     3000

agcgugaccg accgccucaa gcgggauugg ggagucaaug acguguggaa cuccaucauu     3060

uaucccagau ucgagcgccu gaaugaacuu acaggcagcc agcaguucgg gugcugggag     3120

aacaaggaag gaaaacgggu guuucaaacc gccgucccgc uggagaugca gaagggcuuc     3180

ucuaaaaaga gaaucgauca ccggcaucac gcuauggacg ccauuguuau cgcuugugcc     3240

auccgcaauc auauuaacua ccucuccaau gaaagcgccu ccaaaaaggc uaagaucucu     3300

agaauugauc ugcagcggcu gcuuugcaca aaaugucgca ccgacgacaa cgggaauuau     3360

aaguggaucg ugaaaaagcc uuggcccgcg uuuacgcagg auauuagaaa agcccuggag     3420

aacaucacag ugagcuucaa gcaaaaucuc cgggucauua acaagaccac aaauuacuau     3480

gugcacuuug acgaguccgg caaaaagguu cuggaaaaac agaucaacgg agauucuugg     3540

gcuaucagaa agcccaugca caaagacacc guguucggca aggucaaucu ccagaaagug     3600

aaggagguuc gccugagccu ugcccuggau accccuaaga ugauugugga caaaaagcuc     3660

aaagaaaagg uccugcagcu guacuccuau aaauacgaua agaagcggau cgacaaauac     3720

uuuaaggaga acgccucucu uuggaaagau cugaaccuca gcaagguggc uguuuauuac     3780

uucacaaaug acuccacgga accgcuggug gccgucagaa aaccccucga uaccagcuuu     3840

aacgcgaaga agauuaaaga gucugugaca gacaccggga uccaaaagau ucugcuuaau     3900

caucugucca acaaucagga uaacccuaau cucgcuuuca gccccgacgg aaucgacgag     3960

augaaccgca aucugacaac ccugaacgau ggcaaauauc accagccuau cuacaaggug     4020

cgggucugcg aaccccaggg gaauaaauuu ucuguuggaa cguccggcaa caagacaagc     4080

aaguaugugg aggccgccaa agggaccaau cuuuucuucg cuguguacca aaccgaagac     4140

ggcaagagaa acuaugagac aauuccucug aaugucguga ucgagcgcga aaaacucgag     4200

ugggcccccg uuccggaaaa caauaacaac ggagauaagc ugcucuuuug gcugucuccu     4260

aaugaccuug uguacguccc caccgcggau gagcugaaca auggcaugcg gcuggacaac     4320

auggauaaag ggagaauuua caagaugauc uccugcagcg gaccucagug uuucuuuauu     4380

ccccagaaug uguccaaccc gaucaucccu guuauugaac ucggcgugaa uaacaaagcu     4440

gaaaagagcu ggaauaacga gaugaucaag gucgugugcg uccccauucg cguggacaga     4500

cuggggugua ucacacaaau uaauaaccag aaauga                               4536


<210>  4592
<211>  4539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4592
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuaucgaa       60

aauuccuuca cagaaaagga gggcaaaauu aacgauaugg gggugagaau cauucccaug      120

ucugcugaca uccugggaaa guuugaugcc ggccagagcc acucccaaac ggcggaacgg      180

accggguacc gcggaacaag acggcucuau cagcgcgaca aucugagacg ggagcgccuu      240

cauagagucc ugcacauccu cggcuuccug ccuaaacauu acgaugaaca cauugacuuu      300

gagaagcggc ugggccaguu caaggagggg aaagaaauca agcucaacua ucgcugcaau      360

guugagaaca aacaugaauu uauuuucauc ucuagcuuua augagaugcu gaccgaguuc      420

aagaaagcuc agccggaacu guuuuaccuu aaguccaacg gagaggaaac aaagauuccc      480

uaugauugga cccucuacua ucugagaaag aaggcccuuu cucaaccucu gacaaaacag      540

gagcucgccu ggaucauucu gaauuucaac cagaagcggg gcuacuacca gcugcgcggg      600

gaagagaucg acgaugacaa aaauaagcaa uuugugaaac ucaaggugaa ggaggucauc      660

aacagcggag aagcugugaa aggcaagccc cuguucgaug uuauuuuuga gaaugggugg      720

aaauaugaca aacagguggu caaaaccgaa gauuggaucg acagaacgaa ggaguucauu      780

gugaccacaa agguucuuaa aaacggagac cugaagcggu ccuacaaaac cguggauagc      840

gaaaaggacu gggccgcuau caaagccaag acagagcagg auauugagcg cucuaauaag      900

accgucggcg aguucaucua ugagacgcuc cuggaaaacc cuacacagaa aaucagaggc      960

aaacuuguga aaaccauuga gcggaaguuu uacaaagagg aacugaagaa cauccucauu     1020

aagcaaaucg agcugcagcc cacacuguuc uccgaggaac ucuauaaagc guguauugag     1080

gaacuguacc cuagaaauga ggcccaccag aacgcuauca agaaucgcga cuuuaucuau     1140

cuucugaugg augacauuau cuucuaccaa cggccgcuca aaagccagaa gucuaacauu     1200

uccgggugcc agcuggaaca gagaguguac uauaaagcca auaaggauag cggagagaac     1260

gaagagguca aacaagcugu uaaggccauc cccaagucuc acccucuuuu ucaggaguuc     1320

cgcaauuggc aguggcugca gaaccucaaa auuuacaaua agguggacac cgaaaaaggc     1380

gagcugaccg augugacaaa gcaacugcuc cccuccgaag acagcugggu caaccuguuu     1440

aauuuccuuc aguguaaaaa ggagcuggaa cagaagcagu uuaucaaaua uuucauugau     1500

aagaaacuca ucaacaagac ggagaaagac cauuaccggu ggaauuaugu ggaagacaag     1560

aaguacccuu uuaccgagac aagagcgcaa uucaucuccc gccugaccaa aguugagggg     1620

auugaagaga ucaacacauu ucuggauaag aaaacccaga uuggaacgaa ggacaauucu     1680

cccuucguga gccggaucga acagcuuugg cacauuaucu auuccgucag cgauaucaaa     1740

gaguacgaau cugcccucga gaaguucgcu auuaagcauc accugaacga ggaauccuuu     1800

guggccaacu ucaucaaauu uccgccuuuc aagagcgacu acggcucuua uagcaaaaag     1860

gcucugaaua aacuccugcc ccuuaugaga cgcgggaacu acuggacaga gaaugauauu     1920

uccgaaaagg uuaaacugcg ggugucugag aucauggaga gagucaacgc ccucaaucug     1980

aaggaagacu auagcuccaa ggagcuggcc gaaacccuug ugaacgucag cgaugacgau     2040

gugaaaaagc agcucauuaa aucuuuuguu uccuucaagg accgcaaucc ucugauggga     2100

cugaacaccu uucaagcuac auaccucgug uauggccggc acagcgaggu cggcgauauc     2160

cagaauugga aaacccccga cgauaucgac acauaccuga agaacuucaa gcagcauucu     2220

cuuagaaauc ccauugugga acaaguugug acggagaccc ugcgggucgu gcgcgacauc     2280

uggaaacacc augggaaguc ccagacaaac uuuuucaaug agauucacgu ggaacucgga     2340

agagagauga aaaacccugc ggauaagcgg aaaagaauca gcgaacgcaa uaccgagaac     2400

gaaaacacaa auaaccggau uagagagauc cugaaggacc ugaugaauga ugccuccauc     2460

cagggcgacg uucgcgauua uucuccgagc cagcaaggga uucuuaagau cuacgaggaa     2520

ggagucuacc agaaccccaa aguggacuau uccaaggugu cugaggauga aauuaccaaa     2580

aucagacgga gcaauaaccc uacgcagaag gagauucagc gcuacagacu cuggcuggaa     2640

caaggcuaua ucucccccua uaccugcaaa ccuauucccc ugagcaagcu cuuuacacau     2700

cacuaccaga ucgagcacau cauuccgcag ucucgguacu ucgacaauuc cagcucuaac     2760

aagaucauuu gugaguccga agucaaugag gauaaagaca acaagaccgc uuuugaauau     2820

cugaaagaua agggggagaa ugugaucaac ggccauaaac uucugagccu cgccgccuac     2880

gaggcucacg uuaauaagua cuucaagaac aaucgccaga aacugaagaa ccugcuuucu     2940

gaagacauuc cugagggauu caucaacaga caaaugaaug auagccggua uaucuccaaa     3000

cucgugaagg gccugcugag caacauuguc cgcgaagagg gggaacagga ggugacaucu     3060

aaaaaucuca uccccguuac cggauccgug acgagcaagc ugaaaaacga cuggggccuu     3120

aaugacaagu ggaacgagcu gauucucccu agauuuaagc ggcugaauca gcugacacaa     3180

accaacgauu ucacaaccgc caauaccaac gggaauacaa uccccacggu cccugacgaa     3240

cuucugaaag gauuuuccaa gaaacgcauu gaucauagac accacgcgcu cgacgcucug     3300

gugguugccu gcuguacccg gaaccauucu aauuaccuca gcgcccugaa cacagagaau     3360

aagaacuaug gccuuagaga uaaacugcuc aucaagaaug acaacgauca cuacaccaag     3420

uccuuccaga ugcccuggca uggguuuaca accgaagcua aaaaccagcu ggaggaaacg     3480

gugaucucuu ucaagcagaa ucugcgcguc auuaacaaag ccaauaacaa guuuuggagc     3540

uauaaagacg agaauggcaa acuuaaccug gauaagaaug gaaaaccgau caagaaacuc     3600

cggaagcaaa caaaaggcga caacugggcu gugagacagc cucugcacga ggaaaccauu     3660

uccgggaucg ugaaucuccc cugggucaag gccgagaagg aaaaauucac cuacgcgaca     3720

cgcaagucuc uggauaagag cuuugaccuu aagaaaauug agaagaucac cgacacaggc     3780

auucagaaaa uccugcggaa cuaucucaug caggaaaagu ucaaggagcu ggauaaaaag     3840

gacaaagagg uuuacaauuc cgaacuggcc uuuagccaag agggacuuga agaucugaac     3900

ucuaauaucg agcaguucaa cgaccagaag ccccauaaac cuauuuacaa ggugagacuc     3960

uucgagaagg gguccggacg cuuugcucug ggcuucaaag ggaucaaugc caagaaauau     4020

gcucagggca gcccgaaccu cuacuuuaac auuuaucgga augaagaugg acaauacuau     4080

gagacggugc cccugaacaa ggucaucauu caccagaaaa augccgcgaa caugccuaag     4140

aagaauagaa cccccgugcc uaucucuaac acagccauca aucgcuccaa agaaguucuu     4200

guggacuaca agaugacccu gagcccccug gaucucgucu auauuccuac agaggaggaa     4260

cugaacaaua ucaacucucu ugacuucaau aaccugucca aaagccagcg gaagagacuc     4320

uacaauguga acgauuuuuc cggcuacacc auuuauuuca gccccaauac gcuggcuaaa     4380

ucuaucugcc cgaaggaggu ugacaugaag uuugaugaaa agaagcagaa accuaccggg     4440

agcuucgacu ccaagacagc cucucucgau ggaaugagca uuaaagagcg guguaucaag     4500

cugaucauug accgccuugg caacaucucc aaagcuuga                            4539


<210>  4593
<211>  4539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4593
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cguggucaau       60

gcugaugaga ucacaagaga cgauggcucc cgguaccuua agcccaacgg gauuucugcc      120

gcgggaagcc gcaucauucc uauguccgcu gacauccugg gcgauuucga aaaagggaau      180

agcaucagcc agacggccga gagaacccgg augcgcaugg ccagacggcu ccacgaacgc      240

gcucugcuua gacgggagcg ccugcucaga guucugaacc ugcucgacuu ucugccgaag      300

cauuucgccg auaaaauuga ccgguaugga aaguuuacag augacuccga acccaagcug      360

gcuuggcgca aaaauaccga gggcaaguac gaguucaucu uucaagaugc cuucaacgaa      420

augcuggcgg aguuuaaaga caagcagccu gaaauugugg aggagggcaa aaagaucccc      480

uaugauugga caauuuacua ucucagaaag aaagcccugg aaaaggcucu uucuaaagag      540

gaacugagcu ggauccuccu gcaguucaau cagaagcggg gguacuauca acugcgcgga      600

gaggaagagg agauuccuca ggacaaaaag aucgaauacc ucgcccagaa aguggucaag      660

guggaggcua ccgaccagaa gaaaggcgau gacguuuggu acaacgugua ucuggaaaau      720

gggaugaucu acagacggac auccaaggcg ccccuugauu gggagggaaa agucaaggaa      780

uuuauuguga ccacggaggu ugagaacgac ggcgaaaccc cuaaaaagga uaaggagggg      840

aauaucaaaa gaucuuuccg cgccccgaag gaagacgauu ggacacugcu caaaaagaaa      900

accgaggccg acauugaaaa cucucacaag acagugggau gcuauaucua cgauucccug      960

cuuaauaacc ccaagcagaa aauuaucggc aagcuggucc ggaccgugga gagaaaauuu     1020

uauaaggagg aacucacgca gauccugaaa aagcaagugg agcugauucc ugaacuccgc     1080

gacgauaauc uguacaagca guguaucgag gagcuuuauc ccauuaacga agcucaucgg     1140

aacacaaucg ccaaaaccga cuucgcuaau cuguuuauua acgacauccu cuucuaccaa     1200

agaccucuua agagcaaaaa gucucagauc gauaauugcc ccuacgagga acgcauuuuc     1260

aucgacucca aaacaggcga gaagaaaaag gucccgguua aguguauugc caaaagcaac     1320

ccucuuuuuc aggaguuccg gcuguggcaa uuuauccaga aucucagaau uuaucagcgc     1380

gaaaaggaga ucgaugggaa acugcugacc gacauugaua ucaccucuga augccucaag     1440

uccgaggaag acuacgugcg gcuguucgau uggcuuaacg acagagagac aaucgaacag     1500

gaggaucugc ucaaacaccu guuuaauacg aaaaagagca agaacaagga gaaucccuau     1560

cgcuggaacu acguggaaga caaagucuau ccuuguaaug agacccgggc cacaauucug     1620

aagggauucu ccaaaugcgg caucaacgcu ucugugcuua gccccgaacu cgagauggcg     1680

cuguggcaua uucuguacuc cguugaagau aagaaggaga ucgagaccgc ccucacacac     1740

uuugcucaaa aacaggggug gaaugacgau uucgccaagg uguuuagcaa agccaagccc     1800

uucaaaaagg acuauggauc uuacuccgaa aaggcuauua aaaagcugcu uagccugaug     1860

agaaugggca aauacuggaa ccaggacaau aucgauaaga acacccucga ccggaucgau     1920

aaaauuauca acggggagua ugacgaaaag auuucuaauc gcgucagaga uaacgccauc     1980

aaucugaaag acauuagcga uuuucgggga cugccugugu ggcuugcgug uuacaucguu     2040

uaugacagac acuccgaggc uaaggauugc acgaagugga acucuccgga ggaaaucgac     2100

agcuaccuca aaaaguucaa acagcauucc cugcgcaauc ccauugugga gcaagucgug     2160

acagaaaccc ugcggaccgu cagagauauc uggauucagg aggaacagau cgacgagauu     2220

caccucgagc ugggccgcga ucuuaagaac ccugccgaca aaagaaagaa gaugagcgaa     2280

aauauccuga aaaacgagaa uacaaaccuc cggaucaagg ccaugcugau ggaauuuaug     2340

aaucccucua ugggcauuga gaacgugcgc ccuuauuccc ccagccagca agacauccug     2400

agaauuuacg aagagaaugc ucuugauaac cucaccaaag acgauaagga cuucgauuuc     2460

aucucuaaaa uuuccaagca ggcccagccg agcaagacag acaucauucg guauaaaugu     2520

uggcuggagc agaaguaccg cuccccuuac acggggaaaa ccaucucucu gagcaagcuc     2580

uuuacacccg cuuaugaaau cgagcauguu auuccucaau cccuguacuu cgaugacucu     2640

uuuagcaaua aagugaucug cgaagccgag gucaacaaac uuaaggauag acagcuggga     2700

uauaaguuca uugcggaaca ccacggcgag aaggugcagc ucucccaagg ggagguugug     2760

gaaauccuga gcgucgacgc cuacgagaaa uuugugaagg aaaacuaugc uaauaaccgg     2820

auuaaaauga agaagcugcu uauggagucu auccccgaug aguucaucga acgccagcuc     2880

aaugacucca gauacauuag caaagugguu aagggacugc ugucuaacau cguccgggag     2940

aaaauugacg augaaaauua ugagccugaa gccgugucca agaaccucau cagcuguaau     3000

ggcgcuauua ccgaccgccu gaaaaaggau ugggggauga acgacgugug gaacucuauc     3060

auccuuccca gauuuauucg gaugaaucag aucacaggca aagauugcuu caccacgacc     3120

aaugccgagg gacaucugau uccgcagaug ccucucgagc ugcaaaaggg cuuuaacaag     3180

aaaagaaucg accaccgcca ucacgcgaug gaugccauug ucaucgcuug cacaacccgg     3240

gaccauguga aucugcuuaa caaugaagcc gcucacagca aguucaacgc cacaagauac     3300

cagcugcagc gcaaacuccg gagauuugag aaggccguua uugaugggaa agaacgcgag     3360

guggcuaagg aguuccugaa gcccugggac uccuuuagcu cugauuccaa aaacauccuc     3420

gaaaauauca uugucagcuu caagcagaac caacggguga ucaauaaaac cacgaacgcg     3480

uuccugcacu uugacgagaa uggaaagaaa acauucauua agcagggcaa gggggauucc     3540

ugggccauca gaaaaucuau gcauaaggac accguuuuug gagaaauuaa ccuucgcaaa     3600

gugcggagcg ucucccugaa ugaggcuauc aaggugcccg aaagaguucu caacaaaaag     3660

aucaaggaga aaauucugga acugaagaau aacaaaaucg acgccaagaa uauuaaaaag     3720

uacaucgagg agaaccacac aggcggguau ggcauugauu cuagcaaaau cgacguguuc     3780

uacuuuacca aggaaaccaa ggagcgguuc uuugccacac gcaaaacgcu ugauaccucc     3840

uucaaucagg cuaagaucaa agacucuauu gccgauagcg gaauccagaa gauucugcuc     3900

gcgcaucuga aauccaagaa cggcgacgcu gaacaagccu uuagcccuga ugggaucgac     3960

gagaugaaua agaacauugu cgagcucaau aacggaaaau uccaccagcc gauccugaag     4020

gugagagugu augaaaaagc cgauaaguuu gcugucggcc agaaagggaa caagaagguu     4080

aaauucgugg aggccgcgaa gggaacaaau cuuuucuuug cuauuuucga aaaagacggc     4140

aagcggucuu accugaccau cccccucaac gugaugaucg auugucaaaa acaguauggg     4200

aaucagugga agaacaauau ugaggccuac cugaaggaaa aagagcuggu cgagaaggac     4260

gugaaacuuc aguuuauccu guccccuaac gaucucguuu aucugcccac agaaggcagc     4320

auugacaaga aacgcaucua caaggugguc ucuuucacca auaacagacu cuacgccauu     4380

ccuuaugcua ucgccaaauc caucguggac aagaaugagu uuacgcaacu gaacaagauu     4440

gaguucacag auaauaaaga aagcaucaag gacgcuugcg uucccgugau uaucgaucgg     4500

cuuggaaacg ucauugaauu uaauggcaaa agacuguga                            4539


<210>  4594
<211>  4539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4594
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugggc cgugaucaac       60

uccgaggaag auggccccau ugucaaaaag agaaucgagc uugcuggguc ucggauuauc      120

ccuauggacg ccgcgguucu gggaaauuuc gauagcggcg ugacaaaauc ccagacggcu      180

gaacgcaccu cuuuuagaag cauccggcgc cucagagagc ggcaacugcu ucgcagagaa      240

cggcugcaca aggugcucca gauucugggg uuccugccgg agcauuacgu caacgacauc      300

gauuuugaga aucaccccgg aaaaauuaag cgcaacacag aaccuaagcu ccccuggcgg      360

aaaaauucca ugggccaguu cgaguuuauc uuccaggacu cuuuugaagc caugcugacc      420

gauuucgcca cacaucaacc uguguuuguu agcgagggca agaaagugcc cuaugacugg      480

acccuuuacu aucugagaaa gaaagcucuc acagagggga uuuccaagga ggaacuggcc      540

uggcuuaucc ugaacuucaa ucagaagcgc ggauacuauc agcucagagg cgaagaggaa      600

gaggagcaga gcaacaaacu ggucgaauuu uacgcucugc gggugguuaa gguggaggcc      660

accgaugaca aaaaggggaa agauacgugg uauaaugucc accucgaaaa cggauggauu      720

uaccgcagaa ccucuaauau cccucuggac ugggagggca aggugaaaga auuuaucgug      780

acaaccgauc uuaacgagga cgggacaccg aagcuggaca aguacggaga ggucaaacgg      840

uccuuuagag cgcccaagga agaugacugg acccucauua aaaagaaaac ggagagcgau      900

aucgccaauu cucauaagac aguuggcugc uauauuuacg acgcucugcu ucaaaacccu      960

caccagaaga ucaaaggccg ccuggugcgg accguggaaa gaaaguucua uaaagaggaa     1020

cuccgccuga uucuggagaa gcagcucucc uuccaccccg agcugcagga ucgggaccuu     1080

uacagagccu guaucgaugc ucuguauguc agcaacgaag cccauaaaaa uaacaucucu     1140

gggcgcgacu uuguguaccu cuucauugau gacaucauuu ucuaucaacg gccucugaag     1200

acaaagaaau cccuuaucga uaauugcccc uacgaggaaa accaguacau ggacagagag     1260

accggagagg uuaagcaggc gccgauuaaa uguaucgcca agagccaccc ucuguuccaa     1320

gaauuucgcc ucuggcaguu caucuccaau auucggaucu aucagagaga gcgcgugcug     1380

gacgauggca aacuggaaac cgacgucgau gugacacccg acuuucucaa gacggaggau     1440

gacuacacca cacuguucga augguuuaac gaucggaagg agguugacca gaaggcuuuc     1500

cuuaaaaauc cucaauuugg gcugaaaaag accuucacag aguauagaug gaacuacgug     1560

gaagauaaau cuuaucccug caaugagacc cggaacgcca uucucaaugg acugcagaag     1620

gcuggcguca gcgccgacuu ucugucccgc gaaguggagg aagcccuuug gcauauccuc     1680

uacagcgucg aggauaaaau ugagaucgaa ucugcucuga gaaaguucgc guccaagcac     1740

gaccuggaug accgguucgc cgacguguuu aaaaagcucc cucccuucaa aaaggaguau     1800

ggggcuuaca gcgccaaagc cauuaagaag cugcuuccgc ugaugagaau cggaaaauac     1860

ugggaacucu cuaacaucga ugacaagacg aaagagcgca uugauaagau cauuacaggc     1920

gaaugggacg agaauaucca cgaucggguu agagagaaag cuauuaagcu gagcgacauc     1980

accgauuuua agggccugcc ccuuuggcuc uccuguuaug uggucuacaa ccgccauucu     2040

gaagccagag cgaucgcuaa augggagagc ccugaagaca uugauuccua ucugcggcug     2100

uuccgccagc acagccucag aaaucccauc guggagcagg uuauuaccga gacacugcgg     2160

accgugcgcg acaucuggaa gagagucggg caaauugaug aaauccaugu ggagcuugga     2220

cgggaaauca aaaacccugc cgacaagcgc gccaaaauga cagcucagau uguggagaac     2280

gaaaauacga accugagaau caaggcccuc cuggcugagu uuauuaauuc ugaguacgaa     2340

aucgagaacg uucggcccua uuccagcaau cagcaggaaa agcugaaaau uuacgaggau     2400

accguccuua acggcgugaa ggaaaucccg gaggacauca auacaauucu caaaaaguuc     2460

cgcgagucug accugaaaaa gagaccuacc aaauccgauu uucugcacua uaagcucugg     2520

cuggaacaaa aguacugcag ccccuacaca gggcggauca uuccucuugg aaaacuguuc     2580

accuccgccu augagaucga acauauuauc ccccaggcgc acuacuuuga cgauucuuuc     2640

agcaacaagg ugauuuguga ggccgaaguc aauggccuca aagacaagaa acugggguau     2700

gaguuuauca agcacaaccc uggcaagaug guggagaucg gauucggcaa acccccgguu     2760

agaaucuuuu ccguggaaga guacgaacgc uucgucaagg agaauuauuc ugggauuaaa     2820

aacaagauga auaaacugcu uauggaugac auuccugaga cguucaucga acggcagcuc     2880

aacgauagca gauacauuuc caaggugguu aagagccugc ugucuaauau cgugcgcgag     2940

aaagacucca acggagaaua ugagcccgaa gcuaucagca agaacgucau ugugugcacc     3000

ggcucuguua cagaucggcu caaaagagac uggggaguga augaugucug gaacuccauc     3060

auuuaccccc gcuuugagcg gcugaaugag cuuaccggga gccagcaauu cggcuguugg     3120

gaaaacaagg aggggaaaag aguguuucag acagccgugc cucuggaaau gcagaagggc     3180

uucucuaaga aacgcaucga ccaucggcac caugcuaugg acgccauugu caucgcgugc     3240

gccaccagaa aucacaucaa cuaccucagc aaugaguccg cuagcaagaa agccaagauu     3300

ucucggacgg aucugcaacg ccugcuuugu acaaaaugca gaaccgacga uaacggaaau     3360

uauaagugga ucguuaagaa accguggccc gcuuuuacac aggacauucg gaaggcccug     3420

gaaaacauca ccguguccuu caaacagaau cuccgcguga uuaacaagac cacaaauuac     3480

uauguccacu uugaugagag cggcaaaaag gugcuggaga aacagaucaa cggggacucc     3540

ugggccauua gaaagccuau gcauaaggau acgguuuucg gaaaagugaa ucuccaaaag     3600

gucaaagaag ugcggcuguc ucuugcucug gacaccccca agaugaucgu ugauaaaaag     3660

cucaaggaga aagugcugca gcuguacagc uauaaguacg acaaaaagag aaucgauaaa     3720

uauuuuaagg aaaacgcguc ccuuuggaag gaccugaacc ucucuaaagu cgccguguac     3780

uacuucacaa augauagcac cgagccucug guugcugugc gcaagccccu cgacacaucc     3840

uuuaacgcca aaaagauuaa agaaucuguc accgacacgg gcauccagaa gauucugcuu     3900

aaucaccuga gcaacaauca ggaugacccu aaccucgccu ucucccccga ugggaucgac     3960

aagaugaaua aaaaccugac agcucugaau gauggaaagu aucaucaacc gauuuacaaa     4020

gugcgggugu gcgagccuca gggcaacaag uucagcgucg ggaccucugg caauaaaacc     4080

uccaaguaug uugaggccgc gaaaggaaca aaccuguuuu ucgcugugua ccagaccgaa     4140

gacggcaaga gaagcuauga gacaaucccc cuuaaugugg ucaucgaacg cgagaagcuc     4200

ggguggacgc cugugcccga gaacaauaac aauggagaua aacugcucuu uuggcugucu     4260

ccgaacgacc uuguuuacgu gccuaccgcc gaugaacuga acaauggcau gcggcuggac     4320

aacauggaua aggggagaau uuauaaaaug aucuccugua gcggacccca gugcuucuuu     4380

auuccucaaa augucucuaa cgccaucauu cccgugaucg agcucggcgc uaauaacaag     4440

gccgaaaaau ccuggaauaa cgagaugauc aagguugugu guguccccau ucgcguggac     4500

cggcuggggu gcaucacaca gauuaauaac cagaaguga                            4539


<210>  4595
<211>  4551
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4595
augaagaaca uccugggccu cgaccugggg accaauagca uuggaugguc cgugauccag       60

gagaaacaca acgaaaauaa cgagugccaa aaacuuauuc ugggcgccgg gucuagaauc      120

auucccaugg augcugccgu ccucggagac uucgcgaaag gcaauacaau cagccagacg      180

aaggaucgga cccaguaccg cgggguuaga cggcugcgcg aaagaugucu ucugcggcgc      240

gagagacucu uucgggugcu ggacaucaug ggauuccugc cucagcauuu uuccaacgcu      300

cucacacgcu auggcaaauu caaggaugcc gacgaggaac cgaagauugc cuggagaaaa      360

gaugacgagg gcaagaugca cuuucuguuc gcugauucuu uuaaugaaau gaucgaggag      420

uuccggagcg ugcaacccga acuucugaaa cgcgggauga aggucccuua cgacuggacc      480

cucuauuacc ugcggaaaaa ggccaugcgg gaggcuguga cacccuuuga acuugccugg      540

cugauucucu ccuucaacca gaagcgcgga uauuaccagc ugagaggcga ggaagagggg      600

gaagcggaua auucuaaaag cgaggaguau cuggcccagc ggguugugga cgucaucgau      660

accggagaaa agaaaggcaa guccacaugg uuugacauug ugcucgagaa cgggaugguu      720

uaccauagac cugcugaaau caaacccgac uggauuggaa agaccaaaga guucaucgug      780

acgacccaau uugauaagga cggcaauccu aagaaagaua aggaaggcaa cgucaaaaug      840

agcuuccgca ugccgaauga gaacgacugg aaucugcaga agaaaaaggc cgagaaggau      900

aucgacgaau cugggaugac agugggagag uacauuuaua acgcccuucu gcacaacccc      960

aaacagaaga ucaaaggcaa gcucgugcgg gucguugaaa gaaaauacua uaagaaugag     1020

cugcagaaga uucuugaauc ccaaaaacgc uucaucccuc agcugagcga uucugcucuc     1080

uacgagaagu gcauuaaaga gcuguaucag uccaacgaag cguaccggaa uagcaucucu     1140

accagaaacg acuuuacaua ucuguucgug gauaauauca ucuuuuacca acgcccccuc     1200

aaguccaaaa agagccugau ugacaacugu ccuuacgagu cccuugccua uaccaaggau     1260

ggggaggaac auaaagcucc cgugaagugc auugccaaau cucacccgcu guaccaggag     1320

uuccggcucu ggcaguuuau cagcaaucug agaauuuauc agcgccaaaa gcaggucgga     1380

gacaaaguga aacuugacgu ugaugugacg gacgaguucc ugccuacaga ugaccggaga     1440

gaaucccucu uugauuaccu gaacgacaaa accgaaauca agcaggaugu ccugcucaag     1500

gaguucuuua gcaugaaaaa gcccaaaggc aaggacucug uguauccuua ccgcuggaau     1560

uauguugaag auaaauccua ccccuguaac gugacacggg ggaagauccu ggcuaaacuu     1620

aauagagccg gaauuagcgc cgacuuccug ucuagcaacg gcaaugagga gucccucugg     1680

cacauccugu acucugucga agauaagggc gagcugcgga aggcucuugc gcgcuuugcc     1740

gcuaaaaacg ggcucgacga ugccuucacc gaagcccugg cugacauuga gcccuauaag     1800

aaagaauacg gagccuauag cgcgaaggcu cugaagaaac uccugccucu uaugagacug     1860

ggcaaguacu ggaauuauga caacaucgcc ccggccacca aagcucggau uuccaagauc     1920

auugaugggg aguacgacga gaacaucaga aaucgcgugc gggaaaaagc caugagccuc     1980

aacucugcuu cccaguuuca aggacugccc cuguggcuug ccugcuauau cgucuacgau     2040

agacauagcg agcgcgcgga cucuggcaga ugguccagcc cugaagauau ugacaaguac     2100

cucuccgauu ucaagcagca cucucugcgg aaucccaucg uggagcaggu ugugacagaa     2160

acgcugcgca ccgucagaga cauuuggaaa cgguaugggg agaucagcga gauucaugug     2220

gaacucggcc gcgagaugaa gaacccugcc gauaaaagau ccaagaugaa ucggcgcaug     2280

cuggaaaacg agaaugcuaa caugagagcc aaacuucugc ucacagaguu caugaauccc     2340

gaauuuggaa ucgagaacgu ucggucuuac agcccguccc agcaagaacu gcugcgcauc     2400

uaugaggacg gcgcuauuag caaugccggg ucugaaaucg augacgagau uuccgauauc     2460

augagcagac uuucucaggu ggacgucaag aaacagccua ccgcguccga ggugaagcgg     2520

uacauucucu ggcuggaaca gaacuauaga agccccuaca caggacaaau gaucccucug     2580

gccaaacucu ucacccccgc uuaugagauc gaacacguga uuccucaguc ucgcuacuuu     2640

gacgauagcu ucuccaauaa gguuaucugu gaggccgaag ucaacaagcu gaaagaccgg     2700

augcuuggcu acgaguuuau uaagcagcac aaaggggaga aggugagccu gucuauggga     2760

ggcacggugg aaauccucuc ccccgaggaa uaugucagac auguggagag ccaauacaag     2820

aauaacaaag cuaagaugcg caaacugcug auggaugaca uuccggauga guucaucaac     2880

cggcagcuua augacuccag auauaucucu aagcucauua ucagccugcu guccaacauu     2940

guucgcgaag agggggaaga ggaagccaca ucuaaaaaug ugaucagcug caacggcucc     3000

auuaccgauc ggcucaagaa ggacugggga aucaaugaug ucuggaacca cauuauccug     3060

ccuagauuuc gccggcuuaa ugagcugacc ggcacagaca aauucaccac aucuaacaag     3120

gaggggcaug aagugcccga gaugccccuc gaacugcaga aaggauuuaa uaagaaaaga     3180

aucgaucacc ggcaucacgc cauggacgcu auuguuaucg cguguauuac gcgcgaccac     3240

gugaaccugc uuaauaacga ggccgcuaug agcaagaaua acgccaauag aauccagcug     3300

uccaagaaac uccggcgcua cgaaagcguc gagauugaga aggauggcau cagaaaacaa     3360

aucucuaugg ccaaggaguu caaaaagccu ugggaagggu uuaccgcuga cguggaacgg     3420

acacugagag auauuguugu guccuucaag cagaaccucc gcgucaucaa caaaaccaca     3480

aauuauuacc agcauuuuaa ggacggaaag aagauuuaua gcccgcaguc uaaaggcgau     3540

uccugggcca ucagaaagag caugcacaaa gagaccgugu ucggggaaau uaacaucaga     3600

cgcaucaagg aggugucucu gaaggaggcg augaaacgga uuaaggacau cgcugauaaa     3660

gaacuuagau ccaagauugu cgagcugaug gcccuccaau acaaugaaaa gaagaucaag     3720

caguacuuca gcgacaacaa agaugagugg uccgacguua aucccaagaa aauuaagauc     3780

cauuauuuua cgaaagaaac caacgagcgc uucuuugcca cacggaagcc ucuggauacc     3840

agcuucgaca agaaaaagau uaaaacacag guggcugaua ccgccaucca gaagauccug     3900

cuuaaucacc ugucugcgca cggcgacaaa gcugagacgg ccuuuagccc cgacggaauu     3960

gaugaaauga acagaaauau cauugagcuc aacgacggca aguuccauca accuauccug     4020

aaggugcgca uuuacgaaaa agccgauaag uaugcugucg ggcagcaggg aaauaaaaag     4080

aaaaaguacg uggaggccgc uaaaggcaca aaccucuuuu ucgccaucua ugcggaggaa     4140

aagaucgacg agaagaccgg ggauacaguu auuaccagaa ccuacgacuc caucccccug     4200

aaugugguca uugaucggga aaaacgcgga cuuucuccug ugcccgagaa cgaaaauggc     4260

gccaagccga aauauguucu gagcccuaac gaccucgugu acguccccaa ugaggaggaa     4320

agagagacag ggcggaucug cgaaccucug gauaaggagc gcauuuacaa aauggugucc     4380

ucuaacaaga agcaagccuu uuucaucaaa agcgaaguug cuuccacgau cguggacaag     4440

uuugaguucu cugcucugaa caaacaggag agagcccuua gcggcgaaau gauuaaggag     4500

aucuguauuc ccaucuccau uaaucggauc ggagaaauua cccugagaug a              4551


<210>  4596
<211>  4554
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4596
augaagaaaa ucgugggccu ggaccucggg accaacagca uuggaugggc ccugaucaau       60

gcuuacauua acaaggagca ccuuuauggc aucgaagccu gcggguccag aauuaucccc      120

auggaugcgg cuauccuggg aaauuucgac aaaggcaacu cuauuagcca gacagccgau      180

cggacguccu accgcgggau cagacggcuc cgcgagagac aucugcuucg gcgcgaaaga      240

cugcaccgga uucucgaccu gcugggauuu cucccuaagc auuauucuga uagccugaau      300

cgcuacggca aauuccuuaa cgacaucgag uguaagcugc cgugggucaa ggaugaaacc      360

ggcuccuaua aauuuauuuu ccaagagucu uuuaaggaga ugcucgccaa uuucacagaa      420

caccauccca uccugauugc uaacaauaaa aagguuccuu acgacuggac caucuauuac      480

cuucggaaaa aggcccugac acagaagauc agcaaagagg aacucgcuug gauucugcug      540

aacuuuaauc agaagagagg guacuaucag cuccgcggag aggaggaaga gacccccaac      600

aaacuggugg aauacuauuc ccuuaaggug gagaaagucg aagauagcgg cgagagaaag      660

gggaaagaca caugguacaa ugugcaccug gagaacggaa ugaucuaucg gcgcaccucu      720

aauauuccuc ucgauuggga aggcaagacg aaggaguuca ucguuaccac agaccuggaa      780

gccgaugggu cccccaaaaa ggacaaagag ggaaacauua agagauccuu ucgggcgccu      840

aaagacgaug acuggacccu uaucaagaag aaaacagaag ccgauaucga caagauuaaa      900

augaccgugg gcgcuuacau cuaugauacg cugcuccaaa agccggacca gaaaauuaga      960

ggcaagcugg uccgcacaau cgagcggaag uacuacaaaa acgagcugua ucagauucuc     1020

aagacccagu cugaguucca cgaagaacug agagauaaac aacuuuacau cgccugccug     1080

aaugagcucu aucccaacaa ugaaccucgc cggaacucca ucagcacaag agacuuuugu     1140

caucuguuca uugaggauau cauuuucuac cagcgccccc uuaagucuaa aaagucccug     1200

aucgacaauu gcccuuauga ggaaaaccgg uacauugaua aggagagcgg ggaaaucaaa     1260

cacgcuucca uuaaguguau cgccaaaucu cauccccucu aucaggaguu cagacugugg     1320

caguucaucg ugaaucugcg cauuuaccgg aaggagaccg acguugacgu gacccaagaa     1380

cuccugccga cagaggcgga uuacgucacg cuuuuugaau ggcugaacga gaaaaaggaa     1440

aucgaccaga aagccuucuu uaaguauccu cccuucggau uuaagaaaac cacaagcaau     1500

uacagaugga acuaugugga ggauaagccu uaccccugca augagaccca cgcucagauu     1560

aucgcccgcc ucggcaaagc ucacauuccc aaggccuucc uguccaaaga aaaggaggaa     1620

acacuguggc auauccuuua uagcaucgag gacaagcaag aaauugagaa agcccuccac     1680

ucuuuugcua acaagaauaa ccuguccgag gaguucaucg aacaguuuaa aaauuucccu     1740

ccguuuaaga aagaauacgg gagcuauucu gcgaaggcca uuaagaaacu gcucccccug     1800

augcggaugg gaaaguacug gagcaucgag aacauugaua acggcaccag aauccggauc     1860

aauaaaauua ucgacgggga auacgaugag aacauucgcg agagagugcg gcagaaggcu     1920

aucaaucuua cggacauuac acauuucaga gcccugccuc ucuggcuggc cuguuaucug     1980

gucuacgauc gccacuccga aguuaaagac aucgugaagu ggaagacccc caaagauauc     2040

gaccuuuauc ucaagucuuu uaaacagcau agccugcgga acccuauugu ggagcaaguc     2100

aucaccgaaa cacugagaac cgugcgcgau auuuggcagc agguuggaca caucgacgag     2160

auucacaucg agcucggcag agaaaugaag aaucccgcug acaaacgggc ccgcaugucc     2220

cagcaaauga uuaagaacga gaauacaaac cugagaauca aagcgcuucu gacggaauuu     2280

cucaauccgg aguucggcau cgaaaacgug cggccuuaca gccccucuca gcaggaucug     2340

cugcgcauuu augaggaggg gguccuuaau uccauccucg aacugccuga ggacauugga     2400

aucauucugg gcaaguuuaa ccagaccgau acacucaaga gacccacccg gagcgaaauc     2460

cugcgcuaca aacuuuggcu ggagcaaaag uauagaucuc cuuacacagg ggaaaugauc     2520

ccccucucca aacuguucac cccggcuuac gagauugagc auaucauucc ucagagccgg     2580

uauuuugacg auucccuguc uaauaaggug aucugcgaaa gcgagauuaa caaacuuaag     2640

gaccgcuccc ucggauacga guucaucaag aaucaccaug gcgaaaaagu ugaacuggcc     2700

uuugauaagc ccguggaggu ccugucugug gaggccuaug aaaaacucgu ccacgagagc     2760

uacucccaca acagaagcaa gaugaaaaag cugcuuaugg aagacauccc cgaucaguuc     2820

auugagcggc agcugaacga cucuagauau aucuccaagg ugguuaaaag ccuccugucu     2880

aauauugugc gcgaggaaaa cgagcaagaa gcuaucucca agaaugucau uccuuguacc     2940

gggggcauca cggaucggcu gaaaaaggac uggggaauua acgaugugug gaauaaaauc     3000

guucuuccga gauuuauccg ccugaacgag cucacagaaa gcacccgguu cacaucuauu     3060

aauaccaaca auacgaugau ccccagcaug ccucuggagc uccagaaggg cuuuaacaag     3120

aaaagaauug accaucgcca ccaugccaug gacgcuauca uuaucgccug cgcgaaucgg     3180

aacaucguga auuaccugaa caaugucucc gccagcaaga acacaaaaau uaccagacgc     3240

gaucuucaga cacugcucug ucacaaggac aaaaccgaua acaaugggaa cuauaagugg     3300

gugaucgaca agcccuggga gaccuucaca caagauacgc ugaccgcucu gcagaaaauu     3360

acagugucuu uuaagcagaa ucuucggguu aucaacaaaa ccacaaauca uuaccagcac     3420

uacgaaaacg gaaagaaaau ugucuccaau caaagcaagg gcgacuccug ggccaucaga     3480

aaaucuaugc acaaggagac cgugcauggg gaagugaacc ugcggaugau caagacgguc     3540

agcuucaaug aggcucucaa aaagccucag gccauugugg aaauggaucu gaaaaagaaa     3600

auccucgcga ugcuggagcu uggauaugac acaaagcgca uuaagaacua cuuugaggaa     3660

aauaaagaua ccuggcagga caucaacccc uccaagauua aaguuuauua cuucaccaag     3720

gagacaaaag auagauauuu cgccgugcgg aagccuaucg acaccucuuu ugacaagaaa     3780

aagauuaaag aaagcaucac agauacgggc auccagcaaa uuaugcugcg ccaccucgag     3840

accaaggaca augaucccac acuggcuuuc uccccggacg ggaucgauga aaugaacaga     3900

aauauucuga uccuuaacaa aggcaagaag caucagccua uuuacaaagu ccggguguau     3960

gagaaggccg agaaauuuac cguuggacag aagggcaaua aaagaacaaa guucguggaa     4020

gcugccaaag ggaccaaccu guuuuucgcc aucuacgaga cggaagagau cgacaaggau     4080

accaagaaag ucauucgcaa gcggucuuac agcacaaucc cccucaacgu gguuauugag     4140

agacagaaac aaggacuguc cagcgcuccu gaagacgaga auggcaaccu ccccaaguau     4200

auccugucuc cgaaugaucu uguguacguc ccuacccagg aagagauuaa caaaggggaa     4260

guggugaugc ccaucgaccg cgaucggauc uauaagaugg ucgacuccag cggaauuaca     4320

gcgaauuuua ucccugccuc uaccgcuaac cugauuuucg cccugcccaa ggccacagcu     4380

gagaucuacu gcaauggcga gaacugcauu cagaaugaau augggaucgg cagcccccaa     4440

agcaaaaacc agaaggccau uaccggagag augguuaaag aaaucuguuu uccuaucaag     4500

guggauagac ucggcaauau uauccaggug gggucuugca uucugacgaa cuga           4554


<210>  4597
<211>  4563
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4597
augaagaaaa ucgugggccu ggaccucggg accaacagca uuggaugggc ccugaucaau       60

gcuuacauug augaguauaa caaggaacac cuuuacggca ucgaggccug cggguccaga      120

auuaucccca uggacgcggc uauccuggga aauuucgaua aaggcaacuc uauuagccag      180

acagccgacc ggacguccua ucgcgggauc agacggcucc gcgaaagaca ucugcuucgg      240

cgcgagagac ugcaccggau ucucgaucug cugggauuuc ucccuaagca uuacucugac      300

agccugaauc gcuauggcaa auuccuuaac gauaucgaau guaagcugcc gugggucaag      360

gacgagaccg gcuccuacaa auuuauuuuc caagagucuu uuaaggaaau gcucgccaau      420

uucacagagc accaucccau ccugauugcu aacaauaaaa agguuccuua ugauuggacc      480

aucuacuacc uucggaaaaa ggcccugaca cagaagauca gcaaagagga acucgcuugg      540

auucugcuga acuuuaauca gaagagaggg uauuaccagc uccgcggaga ggaagaggaa      600

acccccaaca aacuggugga guauuacucc cuuaaggugg aaaaagucga ggacagcggc      660

gagagaaagg ggaaagauac augguauaau gugcaccugg aaaacggaau gaucuaccgg      720

cgcaccucua auauuccucu cgacugggag ggcaagacga aggaauuuau cguuaccaca      780

gaucuggagg ccgacggguc ccccaaaaag gacaaagaag gaaacauuaa gagauccuuu      840

cgggcgccua aagaugacga uuggacccuu aucaagaaga aaacagaggc cgacaucgau      900

aagauuaaaa ugaccguggg cgcuuauauc uacgacacgc ugcuccaaaa gccggaucag      960

aaaauuagag gcaagcuggu ccgcacaauc gagcggaagu acuauaaaaa cgaacuguac     1020

cagauucuca agacccaguc ugaguuccac gaggaacuga gagacaaaca acuuuauauc     1080

gccugccuga augaacucua ccccaacaau gagccucgcc ggaacuccau cagcacaaga     1140

gauuuuuguc aucuguucau ugaggacauc auuuucuauc agcgcccccu uaagucuaaa     1200

aagucccuga ucgauaauug cccuuacgaa gagaaccggu auauugacaa ggaaagcggg     1260

gagaucaaac acgcuuccau uaaguguauc gccaaaucuc auccccucua ccaggaguuc     1320

agacuguggc aguucaucgu gaaucugcgc auuuaccgga aggaaaccga cguugaugug     1380

acccaagagc uccugccgac agaagcggac uaugucacgc uuuuugagug gcugaacgag     1440

aaaaaggaaa ucgaucagaa agccuucuuu aaguacccuc ccuucggauu uaagaaaacc     1500

acaagcaauu auagauggaa cuacguggag gacaagccuu aucccugcaa ugaaacccac     1560

gcucagauua ucgcccgccu cggcaaagcu cacauuccca aggccuuccu guccaaagag     1620

aaggaagaga cacuguggca uauccuuuac agcaucgaag auaagcaaga gauugagaaa     1680

gcccuccacu cuuuugcuaa caagaauaac cuguccgaag aguucaucga acaguuuaaa     1740

aauuucccuc cguuuaagaa agaguauggg agcuacucug cgaaggccau uaagaaacug     1800

cucccccuga ugcggauggg aaaguacugg agcaucgaaa acauugacaa cggcaccaga     1860

auccggauca auaaaauuau cgauggggag uaugacgaga acauucgcga aagagugcgg     1920

cagaaggcua ucaaucuuac ggauauuaca cauuucagag cccugccucu cuggcuggcc     1980

uguuaccugg ucuaugaccg ccacuccgag guuaaagaua ucgugaagug gaagaucccc     2040

aaagacauug aucuuuaccu caagucuuuu aaacagcaua gccugcggaa cccuaucgug     2100

gaacaaguca uuaccgagac ccugagaaca gugcgcgaca ucuggcagca gguuggacac     2160

auugacgaga uccacauuga acucggcaga gagaugaaga aucccgcuga uaaacgggcc     2220

cgcauguccc agcaaaugau caagaacgaa aauaccaacc ugagaaucaa agcgcuucug     2280

acagaguucc ucaauccgga guucggcauu gaaaacgugc ggccuuauag ccccucucag     2340

caggaccugc ugcgcaucua cgaggaaggg guccuuaauu ccauucucga gcugccugaa     2400

gauaucggaa uuauccuggg caaguuuaac cagacggaca cccucaagag acccacacgg     2460

agcgagaucc ugcgcuauaa acuuuggcug gaacaaaagu acagaucucc uuacaccggg     2520

gagaugauuc cccucuccaa acuguucaca ccggcuuaug agaucgaaca uauuaucccu     2580

cagagccggu acuuugauga cucccugucu aauaagguga uuugcgagag cgaaaucaac     2640

aaacuuaagg aucgcucccu cggauaugag uucaucaaga aucaccaugg cgaaaaaguu     2700

gagcuggccu uugacaagcc cguggagguc cugucugugg aagccuacga gaaacucguc     2760

cacgaaagcu auucccacaa cagaagcaag augaaaaagc ugcuuaugga ggauauuccc     2820

gaccaguuca ucgagcggca gcugaacgau ucuagauaca uuuccaaggu gguuaaaagc     2880

cuccugucua auaucgugcg cgaagagaac gaacaagagg cuauuuccaa gaaugucauc     2940

ccuuguaccg ggggcauuac ggaccggcug aaaaaggauu ggggaaucaa cgacgugugg     3000

aauaaaaucg uucuuccgag auuuauucgc cugaacgaac ucaccgagag cacacgguuc     3060

accucuauca auacaaacaa uaccaugauu cccagcaugc cucuggagcu ccagaagggc     3120

uuuaacaaga aaagaaucga ccaucgccac caugccaugg augcuauuau caucgccugc     3180

gcgaaucgga acauugugaa uuaucugaac aaugucuccg ccagcaagaa cacgaaaauc     3240

acaagacgcg accuucagac ccugcucugu cacaaggaua aaacagacaa caaugggaac     3300

uacaaguggg ugauugauaa gcccugggaa accuucaccc aagacacacu gacggcucug     3360

cagaaaauca ccgugucuuu uaagcagaau cuucggguua uuaacaaaac aaccaaucau     3420

uaccagcacu augagaacgg aaagaaaauc gucuccaauc aaagcaaggg cgauuccugg     3480

gccaucagaa aaucuaugca caaggaaaca gugcaugggg aggugaaccu gcggaugauu     3540

aagaccguca gcuucaauga agcucucaaa aagccucacg ccaucgugga gauggaccug     3600

aaaaagaaaa uucucgcgau gcuggagcuu ggauacgaua cgaagcgcau caagaacuau     3660

uuugaagaga auaaagacac auggcaggau auuaaccccu ccaagaucaa aguuuacuau     3720

uucaccaagg aaaccaaaga cagauacuuc gccgugcgga agccuauuga cacaucuuuu     3780

gauaagaaaa agaucaaaga gagcaucacc gacacaggca uucagcagau caugcugcgc     3840

caucucgaaa cgaaggauaa ugaccccacc cuggcuuucu ccccggaugg gauugacgag     3900

augaacagaa auauccugau ucuuaacaaa ggcaagaagc accaaccuau cuauaaaguc     3960

cggguguacg agaaggccga aaaauuuaca guuggacaga agggcaauaa aagaaccaag     4020

uucguggagg cugccaaagg gacaaaccug uuuuucgcca ucuacgaaac cgaggagauu     4080

gauaaggaca cgaagaaagu cauccgcaag cggucuuaua gcaccauucc ccucaacgug     4140

guuaucgaaa gacagaaaca gggacugucc agcgcuccug aggaugaaaa uggcaaccuc     4200

cccaaguaca uucugucucc gaaugaccuu guguaugucc cuacacaaga ggaaaucaac     4260

aaaggggagg uggugaugcc caucgaucgc gaccggauuu acaagauggu cgauuccagc     4320

ggaaucaccg cgaauuuuau uccugccucu acagcuaacc ugaucuucgc ccugcccaag     4380

gccaccgcug agauuuauug caauggcgaa aacugcaucc agaaugagua cgggauuggc     4440

agcccccaga gcaaaaacca aaaggccauc acaggagaaa ugguuaaaga gaucuguuuu     4500

ccuauuaagg uggacagacu cggcaauauc auucaggugg ggucuugcau ccugaccaac     4560

uga                                                                   4563


<210>  4598
<211>  4563
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4598
augaagaaaa ucgugggccu ggaccucggg accaacagca uuggaugggc ccugaucaau       60

gcuuacauug augaguauaa caaggaacac cuuuacggca ucgaggccug cggguccaga      120

auuaucccca uggacgcggc uauccuggga aauuucgaua aaggcaacuc uauuagccag      180

acagccgacc ggacguccua ucgcgggauc agacggcucc gcgaaagaca ucugcuucgg      240

cgcgagagac ugcaccggau ucucgaucug cugggauuuc ucccuaagca uuacucugac      300

agccugaauc gcuauggcaa auuccuuaac gauaucgaau guaagcugcc gugggucaag      360

gacgagaccg gcuccuacaa auuuauuuuc caagagucuu uuaaggaaau gcucgccaau      420

uucacagagc accaucccau ccugauugcu aacaauaaaa agguuccuua ugauuggacc      480

aucuacuacc uucggaaaaa ggcccugaca cagaagauca gcaaagaaga acucgcuugg      540

auucugcuga acuuuaauca gaagcggggg uauuaccagc uccgcggaga ggaagaggaa      600

acccccaaca aacuggugga guauuacucc cuuaaggugg aaaaagucga ggacagcggc      660

gagaagaaag ggaaggauac augguauaau gugcaccugg aaaacggaau gaucuacaga      720

cggaccucua auauuccucu cgacugggag ggcaagacga aagaguucau cguuaccaca      780

gaucuggagg ccgacggguc ccccaagaaa gacaaggaag gaaacauuaa acgcagcuuu      840

agagcgccua aggaugacga uuggacccuu aucaagaaaa agacagaggc cgacaucgau      900

aaaauuaaga ugaccguggg cgcuuauauc uacgacacgc ugcuccaaaa accggaucag      960

aagauucggg gcaagcuggu cagaacaauc gagcgcaaau acuauaagaa cgaacuguac     1020

cagauucuca aaacccaguc ugaguuccac gaggaacugc gggacaagca acuuuauauc     1080

gccugccuga augaacucua ccccaacaau gagccuagac gcaacuccau cagcacacgg     1140

gauuuuuguc aucuguucau ugaggacauc auuuucuauc agagaccccu uaaaucuaag     1200

aagucccuga ucgauaauug cccuuacgaa gagaaccgcu auauugacaa agaaagcggg     1260

gagaucaagc acgcuuccau uaaauguauc gccaagucuc auccccucua ccaggaguuc     1320

cggcuguggc aguucaucgu gaaucugaga auuuaccgca aagaaaccga cguugaugug     1380

acccaagagc uccugccgac agaagcggac uaugucacgc uuuuugagug gcugaacgaa     1440

aagaaagaga ucgaucagaa ggccuucuuu aaguacccuc ccuucggauu uaaaaagacc     1500

acaagcaauu aucgguggaa cuacguggag gacaaaccuu aucccugcaa ugaaacccac     1560

gcucagauua ucacaagacu cggcaaggcc cacauuccca aggcuuuccu guccaaggag     1620

aaggaagaga cccuguggca uauccuuuac agcaucgaag auaaacaaga gauugagaag     1680

gcccuccacu cuuuugccaa caaaaauaac cuguccgaag aguucaucga acaguuuaag     1740

aauuucccuc cguuuaaaaa ggaguauggg agcuacucug cuaaggcgau uaaaaagcug     1800

cucccccuga ugcgcauggg aaaauacugg agcaucaaga acauugacaa cggcacgcgg     1860

aucagaauca auaaaauuau cgauggggaa uaugacgaga acauucggga gcgcgugaga     1920

cagaaggcca ucaaucuuac agauauuacc cauuuccggg cucugccucu cuggcuggcc     1980

uguuaccugg ucuaugacag acacuccgaa guuaaggaua ucgugaaaug gaagaccccc     2040

aaagacaucg aucuuuaccu caagucuuuu aaacagcaua gccugcgcaa cccuauugug     2100

gagcaaguca ucacagaaac ccugcggaca gugagagaca uuuggcagca gguuggacac     2160

aucgacgaga uucacaucga gcucggccgc gaaaugaagu cccccgccga uaaaagagcu     2220

cggaugagcc agcaaaugau uaagaaugag aacacgaauc ugcgcaucaa ggcccuucug     2280

accgaauuuc ucaacccgga guucggcauc gaaaauguga gaccuuauuc ucccucccag     2340

caggaccugc ugcggauuua cgaggagggg guccuuaaca gcauccucga acugccugag     2400

gauauuggaa ucauucuggg caaauuuaau cagacagaca cccucaagcg ccccacaaga     2460

ucugaaaucc ugcgguauaa acuuuggcug gagcaaaagu accgcucccc uuacaccggg     2520

gaaaugaucc cccucagcaa acuguucacg ccggcguaug agauugagca uaucauuccu     2580

caguccagau acuuugauga cucucugagc aacaagguga ucugcgaauc cgagauuaau     2640

aaacuuaaag aucggucucu cggauaugag uucaucaaga accaccaugg cgaaaaaguu     2700

gaacuggcuu uugacaagcc cguggagguc cugagcgugg aggccuacga aaaacucguc     2760

cacgaguccu auagccacaa ucgcucuaag augaagaaac ugcuuaugga agauaucccc     2820

gaccaguuca uugagagaca gcugaacgau ucccgguaca ucagcaaggu gguuaaaucu     2880

cuccugucca acauugugag agaggaaaau gagcaagaag ccaucagcaa gaacgucauu     2940

ccuuguaccg ggggcaucac agaccgccug aaaaaggauu ggggaauuaa ugacgugugg     3000

aacaagaucg uucuuccgcg guuuaucaga cugaaugagc ucaccgaauc uacacgcuuc     3060

accagcauua acacgaauaa cacaaugauc cccuccaugc cucuggagcu ccagaaaggc     3120

uuuaauaaga aacggauuga ccauagacac caugcuaugg augccaucau uaucgcuugc     3180

gccaaccgca auaucgugaa cuaucugaau aacgucagcg cgucuaagaa uaccaaaauu     3240

acacggagag accuucagac ccugcucugu cacaaggaua agaccgacaa caacgggaau     3300

uacaaauggg ugaucgauaa gcccugggag acauucacgc aagacacccu gacagcccug     3360

cagaaaauua ccguguccuu uaagcagaac cuucgcguua ucaauaaaac aaccaaccau     3420

uaccagcacu augaaaaugg aaagaaaauu gucagcaacc aauccaaggg cgauucuugg     3480

gcuaucagaa agagcaugca caaagagacg gugcaugggg aagugaaucu gagaaugauc     3540

aagacagucu cccucaacga ggcccugaaa aagccucagg cuauugugga aauggaccuc     3600

aaaaagaaga uccuggccau gcuugagcug ggauacgaua ccaaacggau uaagaauuau     3660

uucgaggaaa acaaagacac cuggcaggau aucaaucccu cuaagauuaa aguuuacuau     3720

uuuacaaagg agaccaagga ccgcuacuuc gcggugagaa aaccuaucga cacgagcuuc     3780

gauaagaaaa agauuaaaga auccaucaca gacaccggca uccagcaaau uaugcuccgg     3840

caccuggaga caaaggauaa cgaccccacc cuggccuuuu cuccggaugg gaucgacgaa     3900

augaaucgca acauucuuau ccugaauaag ggcaaaaagc aucagccuau uuauaaaguc     3960

agaguguacg agaaggcuga gaaauucaca guuggacaga agggcaacaa acggaccaag     4020

uuuguggaag ccgcuaaggg gacgaaucuc uucuuugcca ucuacgagac cgaagagauc     4080

gauaaagaca caaagaaagu cauuagaaag cgcagcuauu ccaccauccc ccugaacgug     4140

guuauugagc ggcagaaaca aggacucagc ucugccccug aagaugagaa cggcaaucug     4200

cccaaguaca uccuuucccc gaacgaccug guguaugucc cuacacagga agagauuaau     4260

aagggggaag uggugaugcc caucgauaga gaccgcaucu acaaaauggu cgauagcucu     4320

ggaauuaccg cuaacuucau cccugcgucc acagccaauc ugauuuuugc ucuccccaag     4380

gccaccgccg agaucuauug caacggcgag aauugcauuc agaacgaaua cgggaucggc     4440

agcccccaau cuaaaaauca gaaggcuauu acgggagaga ugguuaaaga aaucuguuuc     4500

ccuaucaagg uggaccggcu gggcaacauu auccaggugg gguccugcau ucuuacaaau     4560

uga                                                                   4563


<210>  4599
<211>  4575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4599
augaacaaga uccugggccu cgaccugggg accaauagca uuggaugggc caucgugcag       60

aaagaugagc aaggcuucua cgaaaagauu aucaacgcug gguccagaau uauccccaug      120

gacgccgaga caaugaauaa cuuuaauaaa ggaaucacga agucuccugc gagcgaacgg      180

acccgcuuca gaggcgcucg gcgccuuauu gagagauccc ugcuccggcg cgaaagaauc      240

caccggcugc uuaacacaau gaauuaucug ccgccccauu acgccgagaa aauugauuuu      300

gucaaccgcc ucgggaaguu caucggagag gaggaaccua aguaugccua caaaguugac      360

gaacacggca aguuucaguu cauuuuuaaa gagucuuuca augagaugcu gaaggauuuu      420

caggacaaac agcccgaacu ggugcucaac aauaagaagg ugccuuauga uuggaccauc      480

uacuaucuga gaaagaaggc ucuuaaccgg gccauuagca aagaggaacu gggcuggauc      540

auccuccaau ucaauaacaa gcgcggguac uaccagcuga gaggagagga agacgagaca      600

cuuaccgagg gccagaaaaa ggaauauuuu gcucugaaag ucauucgggu ggaggccgau      660

uccucuagcg cggggaagaa gggagaaguu ugguacaaug ugcagcucga gaacggcugg      720

aucuaucgcu gcaaauccga aauuccccug gacugggagg ggaagaucaa agaguucauu      780

gucacaaccg aaguggauga gaacgacaau guucugaagg auaaagaagg aaaggagaag      840

agaaaacuca agaucccuga agccggcgac uggacgcugc uuaaaaagaa aaccgagagc      900

gaccuggaua agucuggcaa gacagugggg gcuuacaucu augacacccu ccuguccaac      960

ccggaucaga aaauuaaggg aggccuuauc cggacaauug agagagaacu guaccgcagc     1020

gagcucgaac aaauccugga caaacagaag gaguuucauc ccgagcugaa agaugggacc     1080

cucuauaaua agugugucga ggaacuguac cggucuaacg aauccagacg cauuagccuu     1140

ucuaauuacg acuucacgcg guucaucaca gaggauguga ucuuuuauca gagaccucug     1200

aaguccaaaa agagccucau ugacaacugc uccuucgaac gccggaauua ccugaacccc     1260

aucaccaaag agcuugccua ugcuccuauu aaguguaucg ccaaaucuca cccccuguuu     1320

caagaguuca gacucuggca guuuauuaag aaucugcgca ucauugaacg ggagaaaauc     1380

gugggagaua agcugauguu cgaccaggau gucacaaccg aauuucuccc gacccaaaac     1440

gacuacguua uccuguucga cuggcuuaau gagcauaagg aaauugauca gaaagcgcug     1500

cucaaguauc cugccuuuaa ccugaaaaag aauaucgaca aauacagaug gaacuaugug     1560

gagaauaaga gcuaccccug caacgagaca cgcgcucugc uuaaggccaa acucaacaag     1620

gcuggcaaua uuccuuccga uuuccuggac aacgauacgc uggaaagccu cuggcacauc     1680

cuguacucug uggaggacaa auuugaaauu gagaaggccc uuaaauccuu cgccaccaag     1740

aaggaacugc gggaggagca gacagucgcu uuugugaaac aguucaucaa uaucccuccc     1800

uucaagaaag aauaugggag cuacucugcg aaggccauua aaaagcuccu gcaacugaug     1860

agaaugggaa gccggugguc cgagaacgaa aucucugaua gcaccaagga ccgcauucag     1920

aaacuuaucg auggcgagug ggacgagucc auuaagacaa gaacccggga uaaagcuauc     1980

aagcucaaug aaaucaacga cuuucagggc cugccccugu ggcucgccag cuauauuguu     2040

uacgauagac acucugaguc cggggacgug augaaauggg aaacgccgga ggacaucgau     2100

uauuaccuga agcaugucuu caaacagcac ggacuucgca aucccauugu ggaaguugug     2160

gucguggaga cacugaaggu cgugaaggac cucuggaaaa ccuauggcua cuuuaacgag     2220

auccauauug aacuggggcg ggagaugaag aauccugccg auaaaagacg cagcaugacc     2280

aacaagaaua ucgaaaacga gaauacaaac cugagaauca aagcccuucu cgcugaacug     2340

gcgaaucaca aggacguuga gggagugcgg cccuacucuc cuucccaaca ggagauucug     2400

aagaucuaug aagagggcgu ccucgauaug cugaccaaag aagaccccga uuacgagagc     2460

auuuccaaga ucucuaaaau ggcccuuccg agcuccucug aacugauucg cuauaagugu     2520

uggcucgagc agaaauacag aagcccuuau acaggacagg ugaucccccu ggcucggcug     2580

uucacguccg aguacgaaau ugagcacauc aucccucaaa gccgcuauuu ugacgauucu     2640

uucuccaaca agguuauuug cgaaagcgcc gugaacaagg acaaagauaa ucuucucgcu     2700

uacgaguaca ucaagcagaa cgagggcaaa auuaucgaaa uuggacuggg caagaaaguc     2760

aagcuguuua ccgccgacuc uuauacagag uucgugcagu ccaauuacgu ggggagcguu     2820

gcuaagaaaa agaaacuccu gauggauacc auccccgacu cuuuuaucaa gagacagcuu     2880

aacgacagcc gguauauuuc ccgcgaaauc augaaacugc ucagcucugu cgugagagag     2940

aaggaagagg aggaagagga agaggaagag gaggccauuu ccaagaaugu gaucgucugu     3000

acaggagcga uuaccgauaa acugaagcgg gaauggggcc ugaacgacgu guggaauacg     3060

aucaucuacc cucgcuucga gagacuuaac caacugacca auagcgauaa auuugggcag     3120

ugggaaaaca agcaggggaa aaagguuuuc caaauugaga ugccccucaa acugcagaag     3180

ggcuuuaaua agaaacggau cgaccauaga caccaugcca uggaugcuau ugugaucgcc     3240

ugcgcuacac agucccacgu caacuaucuc aauaacgaau cugcccauag caauuccaag     3300

gagaaacgcu acuaucugcg gagaaaacuu cgccggauug agauccugga aaaacaggag     3360

cucaaggacg gggugaccau uacaaagaaa aucgaaguug cgaaggaguu caacaaaccg     3420

uggccuaccu ucacgcaaga ugcccacgag gugcugcagu cuaucauugu cagcuuuaag     3480

cagaaccuga gagugguuaa uaaagcuaca aaccgcuacg ccuguuucgu gaauggcaag     3540

aaggaaaucg ucaaacaguc caagggagag ucuugggcua uucggaaacc caugcacaag     3600

gacaccguga gcgccgccgu gucccuuaga aaaaucaaga cagucagccu gucucaagcu     3660

auugaugacu ggaccaacau cguugauaag ucccuccgca aagaaaucgg ccugcucuac     3720

agcaaguaug ggcagaaugg aucuaaaaac auuaucaagu acuuuaaaga ccgggacaau     3780

aagcauaacg gccuggaugu guccaaagug aauguccacu auuucgacaa cgauuacgcg     3840

gccagcagag uggcucuuga ccauaaguuu aauucugcca agauuaaauc caucaccgau     3900

acagggauuc agacgauccu gcucaagcac cuggagagcu auaacgaaau caauggagac     3960

aaaauuaucg agcaccccga gcuggccuuc uccgcugaug gccuugacau ucugaacgcc     4020

aauaucacca agcucaacaa ugggaaauuu cauaagccua uuaagaaagu ucggaagaca     4080

gaaacccugg gccucaaauu caucguggga cagacaggca acaagcgcaa aaaguacguc     4140

gaggcggcug augggaccaa ccuguuuuuc gccauuuaug ugagcguuga cggaaucaga     4200

ucuuacaaga cgaucccgcu ucgggaagug gccgagcgcc ugaaagaagg ccugauuccc     4260

gucccugaga agaaugauaa agacgaucac cuccuguuuu ggcuuagccc cggggaccug     4320

guguacguuc cuuccaucga ggaagaggga agaauugugg aaaucgagaa cauuaagcaa     4380

gaccggaucu auaaagucgu gucuugcaca gaaaaugagu gucauugcau cccccacuuc     4440

guggcuagcc cuauugucaa gaccgaugag cucggcgcca acaauaaggc gcagagagcu     4500

ugguccgggg aaaugaucaa agagaccugc auuaagauca aaaugaaccg ccugggccag     4560

auuguuaaga ucuga                                                      4575


<210>  4600
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4600
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cgugcuuaau       60

gcugccgaug acaacucccu gaaguacauc gaggcggcug gcucuagaau uauccccaug      120

gaugccgcca uucucgggga cuucgaucgg ggaaauagca ucucccagac agcugaacgc      180

acgagaaucc ggggcaugcg ccaccugaga gagcgguauc uucugcgcag agcccggcuc      240

caucgcauuc ugaaccugcu cggguuucug ccuuuccacu acgcucaaga caucgauaga      300

uauggaaauu uuucugccga cauggaaccg aaaauugcgu ggcggaagaa ccuugagggc      360

gaacuggagu ucaucuuuau ugagagcuuc aaugaaaugc ucgaggauuu ucgcaaaaac      420

cagcccgaac uggucgccga caauaagaag guuccuuacg auugggugau cuauuaccuu      480

agaaagaagg cucuggagaa aaagauuucc aaagaggaac ucgccuggau ccugcugaac      540

uucaaucaga agcggggcua uuaccagcuc cgcggggagg acgaugacga aucuaugaag      600

accgcuaaaa caagacaaua cuuugacaag cagaucauua aaaacaucau ugauaccaau      660

cagacauaua agggacagcg gauccugauu guggagcuuc aaaacggcga caaagggaag      720

aucuucaaaa aggaaauccc cgauuggauu ggacaggaga agaauaucau ugucaccgug      780

gacaucgaua aagacggcaa cgauaaguac gaggaaagcg ggucccugag cugcagauuu      840

aaaauuccua cagacgccga gugggaaucu gaguggaagc ucaucaaaau gaagacccag      900

aaggaucugg acgaauccaa caaaacgguu ggaaccuaua ucuacgauag cauucuucag      960

aaucccacac aaaaggugcg cggcaaacug guccggacca ucgagagaaa guauuacaaa     1020

gcggagcuca agcagauucu ggaaaagcag acagaguucc auucugaacu gcaagacaaa     1080

gaccucuaua ucgagugucu gaacgaacuu uacuccaaua acgauaccca ccgcaaugcc     1140

auuacggacc gggauuuugu guaccuguuc augaacgaca uccucuucua ucagagaccu     1200

cugaagucca aaaagucucu uauugauaau ugcccguacg agcgcaacau cuauaucgac     1260

aaagagacag gcgaaauuaa guacuccccc aucaagugua uugcuaaaag ccauccucug     1320

uuucaggagu uccggcucug gaaguuuauc uccucuauua gaaucuauca gcgcgaaagc     1380

guuguggagg ucguggauaa acaacuggac cuguucucca gccagucuac cuccgugcag     1440

aagcucguca cagauguuga cgugaccgau aaauuucuga ccugcgagaa ugacuacgug     1500

gcccuuuucg aauggcugaa caagaaaaag aucauugauc agaaggaguu ucucaaauau     1560

cggcccuucg ggcugaaggg agacaucgac aauuacagau ggaacuacgu cgaagauaaa     1620

agcuauccuu guaaugagac acacgcuaug auucugacgu acuuugccaa ggccggcauc     1680

ucugaagaca uucuuaccaa agagauggag gaagcucucu ggcacauccu guauagcgug     1740

gaggaucgcc aagaaaucac aaaggcgcug cggaccuucg ccuccaagua ccaucucucu     1800

gacagcuuug uuaaaguguu cuccaacauu cccccguuua agaaagagua ugggagcuac     1860

ucugcuaagg ccaucaaaaa gcugcuuucc cugaugagaa ugggaaagua uuggagcgaa     1920

gaugccauug acgucaauac acgggagcgc aucgauaaaa uugugaccgg cgaguacgac     1980

gaaaagauca gagaucgggu uagagagaaa acgauccgcc ucaacgacau ucggcacuuc     2040

agagcucugc cugauuggcu ggccugcuac guggucuaug accgccauuc ugaaacaaga     2100

gagaucaccg aauggaaguc ccccgaggag auugaugaau acauccggaa cuucaaacag     2160

cacagccuuc gcaauccuau uguggagcag gucaucaccg aaacacucag aaccgugcgg     2220

gacauuugga agcagguugg guguaucgac gagauccacg uggagcuggg acgcgaaaug     2280

aagaaccccg cggauaaaag aaagcggauu acaucccaaa ucauugagaa ugaaaacgcu     2340

aaucugcgcg ucaaagcccu ccugacggag uuugugaacc ccgaguucga gaucgagaau     2400

guuagaccuu auucucuugg ccagcaggac auucugcgga ucuacgaaga uggcgugcuc     2460

aacagcgucg ccgaggugcc gggggacauc gcuggcauuc ugaagaaauu uaaugaaaac     2520

gaugccaaga aacgccccac cacauccgag auccugagau auaaacuuug gcucgaacag     2580

aaguaccggu cuccuuauac cggagagaug auuccccuga gcaaacuguu cacauccgcu     2640

uacgagaucg aacaugugau uccucaaagc agauacuuug acgauucuuu cuccaauaag     2700

guuaucugcg aggccgaagu caacaaacuc aaggacaaug cgcuggggua ugaguuuauc     2760

aaaaagcacc auggagaaaa gguggagcuu ggcuucggga aaauugugga gaucuuuagc     2820

gucucugugu acgaacaguu cguuaaggau aacuauuccc gcaaucgggg caaaaugaag     2880

aaacugcuca uggacgagau ucccgaacag uuuaucgaga gacaacugaa cgauagccgc     2940

uacauuucua agguggucaa gacccugcuu agcaacgugg uucgggagaa agccgaacag     3000

gaggcugugu ccaagaaugu caucgugugu acgggaggcg uuaccgacag acucaaaaag     3060

gacuggggga uuaacgaugu guggaauaaa aucguccugc cgcgcuuccu gcggcucaac     3120

gaacugacaa auagcaccaa cuuuacagcc aagaaugcug ccggaaacga gaucccugcg     3180

augccccuug aacugcagaa gggcuucaau aaaaagagaa uugaccaccg ccaucacgcc     3240

auggaugcua ucgugauugc cugcgcuaac cggaauaucg ugaacuaucu caauaacgag     3300

ucugccucca gaaaagccaa gauuagccgg cacgaccugc aggcuauccu gugugauaaa     3360

guccgcaccg acgauaaggg gaauuaccaa uggagaguua agaaaccuug ggacacguuc     3420

acacaggaug uguauguggc gcuugagaac aucauugucu ccuuuaagca gaaccugcgg     3480

gugaucaaua aaaccucuaa cuacuacugg cauuaugaaa auggaaagaa aguucucgug     3540

aagcagguca aaggcgacag cugggccauu cgcaagccca ugcacaagga uacaguguuc     3600

ggggagguua accugagaaa agugaagucc gucucucuca augaagcucu gaaaaacccu     3660

caauuuaucg uggagaagga ccuuaaacgg cagcugcuca gccugcugca gcagaauuuc     3720

gauauuaaga agaucaaaaa guacuuugaa gaacauaaag acaucugguc cgacauuucu     3780

cuuagcaaga ugaacaucua uuacuucacc aaagagacca aggauagaua uuuugccaca     3840

cgcaagcccc uggacgccuc cuucgauaaa aagaaaauug aaagcugcau cacggacacc     3900

ggcauucaac agauccuccu gcggcaccuc gagcugaagg aaaaugaucc ggagagagcu     3960

uuuucuccug acggaauuga ugaaaugaac cgcaauauca uccagcuuaa caaaggcaag     4020

gggcaccauc ccauugccaa gguucgggug uacgagaaag cggacaaguu cgaggucgga     4080

caaagaggca auaaauccag caaguuugug gaagcugcca aagggacaaa ccuguucuuc     4140

gccguguaug agaccgaaua ccccgaugag aagacaaauu cuaucguccg caaacggaac     4200

uacgcuacca uuccucucaa uaucgccauu gagagacaga agcgcggacu guccacggcg     4260

ccggaagacg agaagggcaa cguucccagc uuugugcugu cuccuaacga ucuuguguau     4320

cugcccacau ccgaagacau cgucaauggg cacaucaccc agccuauuga uaaaagcaga     4380

aucuacaaga ugguguccag cucuggcagc cagugcuucu uuauuaaaca uaacaucgcu     4440

aauauuaucg uugacaagua ugaguucagc ccccucaaca aaauggaacg ggccaucucu     4500

aaugagauga uuaaggagau cuguauuccu gugaaggucg accgccuggg aaacaucgug     4560

aaacucggca agagcgaaug a                                               4581


<210>  4601
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4601
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cgugcuuaau       60

gcugccgaug acaacucccu gaaguacauc gaggcggcug gcucuagaau uauccccaug      120

gaugccgcca uucucgggga cuucgaucgg ggaaauagca ucucccagac agcugaacgc      180

acgagaaucc ggggcaugcg ccaccugaga gagcgguauc uucugcgcag agcccggcuc      240

caucgcauuc ugaaccugcu cggguuucug ccuuuccacu acgcucaaga caucgauaga      300

uauggaaauu uuucugccga cauggaaccg aaaauugcgu ggugcaagaa ccuugagggc      360

gaacuggagu ucaucuuuau ugagagcuuc aaugaaaugc ucgaggauuu ucggaaaaac      420

cagcccgaac uggucgccga caauaagaag guuccuuacg auugggugau cuauuaccuu      480

cgcaaaaagg cucuggagaa aaagauuucc aaagaggaac ucgccuggau ccugcugaac      540

uucaaucaga agagaggcua uuaccagcuc cgcggggagg acgaugacga aucuaugaag      600

accgcuaaaa cacggcaaua cuuugacaag cagaucauua aaaacaucau ugauaccaau      660

cagacauaua agggacagag aauccugauu guggagcuuc aaaacggcga caaagggaag      720

aucuucaaaa aggaaauccc cgauuggauu ggacaggaga agaauaucau ugucaccgug      780

gacaucgaua aagacggcaa cgauaaguac gaggaaagcg ggucccugag cugucgguuu      840

aaaauuccua cagacgccga gugggaaucu gaguggaagc ucaucaaaau gaagacccag      900

aaggaucugg acgaauccaa caaaacgguu ggaaccuaua ucuacgauag cauucuucag      960

aaucccacac aaaaggugag aggcaaacug guccgcacca ucgagcggaa guauuacaaa     1020

gcggagcuca agcagauucu ggaaaagcag acagaguucc auucugaacu gcaagacaaa     1080

gaccucuaua ucgagugccu gaacgaacuu uacuccaaua acgauaccca cagaaaugcc     1140

auuacggacc gcgauuuugu guaccuguuc augaacgaca uccucuucua ucagcggccu     1200

cuuaagagca aaaagucucu uauugauaau uguccguacg agagaaacau cuauaucgac     1260

aaagagacag gcgaaauuaa guaccugccc aucaagugca uugcuaaauc ccauccucuc     1320

uuucaggagu uccgccugug gaaguuuauc agcuccauuc ggaucuauca gagagaaucu     1380

guuguggagg ucguggauaa acaacuggac cucuucagcu cccagagcac cucugugcag     1440

aagcugguca cagauguuga cgugaccgau aaauuucuua ccugugagaa ugacuacgug     1500

gcccuguucg aauggcucaa caagaaaaag aucauugauc agaaggaguu ucugaaauau     1560

cgccccuucg ggcugaaggg agacaucgac aauuaccggu ggaacuacgu cgaagauaaa     1620

uccuauccuu gcaaugagac acacgcuaug auucuuacgu acuuugccaa ggccggcauc     1680

agcgaagaca uucucaccaa agagauggag gaagcucugu ggcacauccu guauucugug     1740

gaggauagac aagaaaucac aaaggcgcuc cgcaccuucg ccagcaagua ccaucugucc     1800

gacucuuuug uuaaaguguu cagcaacauu cccccguuua agaaagagua uggguccuac     1860

agcgcuaagg ccaucaaaaa gcugcugucu cucaugcgga ugggaaagua uugguccgaa     1920

gaugccauug acgucaauac aagagagcgg aucgauaaaa uugugaccgg cgaguacgac     1980

gaaaagaucc gcgauagagu ucgggagaaa acgaucagac ugaacgacau ucgccacuuc     2040

cgggcucugc cugauuggcu ugccuguuac guggucuaug acagacauag cgaaacacgc     2100

gagaucaccg aauggaaguc ucccgaggag auugaugaau acaucagaaa cuucaaacag     2160

cacucccucc ggaauccuau uguggagcag gucaucaccg aaacacugcg caccgugaga     2220

gacauuugga agcagguugg gugcaucgac gagauccacg uggagcuggg acgggaaaug     2280

aagaaccccg cggauaaacg caagagaauu acaagccaaa ucauugagaa ugaaaacgcu     2340

aaucuccggg ucaaagcccu gcuuacggag uuugugaacc ccgaguucga gaucgagaau     2400

guucgcccuu auucccuguc ucagcaggac auucucagaa ucuacgaaga uggcgugcug     2460

aacagcgucg ccgaggugcc gggcgacauc gcugggauuc ugaagaaauu uaaugaaaac     2520

gaugccaaga aacggcccac cacauccgag auccuucgcu auaagcucug gcuggaacag     2580

aaguacagau cuccuuauac cggagagaug auuccccuga gcaaacucuu cacauccgcu     2640

uacgagaucg aacaugugau uccucaaagc cgguacuuug acgauucuuu cuccaauaag     2700

guuaucugug aggccgaagu caacaaacug aaggacaaug cgcuuggcua ugaguuuauc     2760

aaaaagcacc auggggaaaa gguggagcug ggauucggca aaauugugga gaucuuuagc     2820

gucucugugu acgaacaguu cguuaaggau aacuauucca gaaaucgcgg gaaaaugaag     2880

aaacuccuga uggacgagau ucccgaacag uuuaucgagc ggcaacugaa cgauagcaga     2940

uacauuucua agguggucaa gacccuucuc agcaacgugg uucgcgagaa agccgaacag     3000

gaggcugugu ccaagaaugu caucgugugc acgggcggag uuaccgaccg gcugaaaaag     3060

gacuggggca uuaacgaugu guggaauaaa aucguccugc cgagauuccu ccgccugaac     3120

gaacuuacaa auagcaccaa cuuuacagcc aagaaugcug ccgggaacga gaucccugcg     3180

augccccugg aacuccagaa gggauucaau aaaaagcgga uugaccacag acaucacgcc     3240

auggaugcua ucgugauugc cugugcuaac cgcaauaucg ugaacuaucu gaauaacgag     3300

ucugccuccc ggaaagccaa gauuagcaga cacgaccugc aggcuauccu uugcgauaaa     3360

guccggaccg acgauaaggg caauuaccaa uggcgcguua agaaaccuug ggacacguuc     3420

acacaggaug uguauguggc gcuggagaac aucauugucu ccuuuaagca gaaccucaga     3480

gugaucaaua aaaccucuaa cuacuacugg cauuaugaaa augggaagaa aguucuggug     3540

aagcagguca aaggagacag cugggccauu cggaagccca ugcacaagga uacaguguuc     3600

ggcgagguua accuccgcaa agugaagucc gucucucuga augaagcucu uaaaaacccu     3660

caauuuaucg uggagaagga ccugaaaaga cagcuccuga gccugcuuca gcagaauuuc     3720

gauauuaaga agaucaaaaa guacuuugaa gaacauaaag acaucugguc cgacauuucu     3780

cugagcaaga ugaacaucua uuacuucacc aaagagacca aggaucggua uuuugccaca     3840

agaaagcccc ucgacgccuc cuucgauaaa aagaaaauug aaagcugcau cacggacacc     3900

gggauucaac agauccugcu ccgccaccug gagcuuaagg aaaaugaucc ggagcgggcu     3960

uuuucuccug acggcauuga ugaaaugaac agaaauauca uccagcugaa caaaggaaag     4020

ggccaccauc ccauugccaa gguucgcgug uacgagaaag cggacaaguu cgaggucggg     4080

caacggggaa auaaauccag caaguuugug gaagcugcca aaggcacaaa ccucuucuuc     4140

gccguguaug agaccgaaua ccccgaugag aagacaaauu cuaucgucag aaaacgcaac     4200

uacgcuacca uuccucugaa uaucgccauu gagcggcaga agagagggcu guccacggcg     4260

ccggaagacg agaagggaaa cguucccagc uuugugcuuu cuccuaacga ucugguguau     4320

cuccccacau ccgaagacau cgucaauggc cacaucaccc agccuauuga uaaaagccgc     4380

aucuacaaga ugguguccag cucugggagc caguguuucu uuauuaaaca uaacaucgcu     4440

aauauuaucg uugacaagua ugaguucucu ccccugaaca aaauggaaag agccaucucc     4500

aaugagauga uuaaggagau cugcauuccu gugaaggucg accggcucgg caacaucgug     4560

aaacugggaa agagcgaaug a                                               4581


<210>  4602
<211>  4584
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4602
auggccaaga uccugggccu cgaccugggg accaacagca uuggaugggc uaucguggag       60

aaagauggca augacuucuc ccuugucgau aaggggguua gaaucuuuuc ugaaggagug      120

aaaagcgaga agggcauuga auccucucgg gccgcggagc gcacagggua cagaagcgcu      180

cggaaaauua aguaucgcag aaagcugcgg aaauacgaaa cgcucaaggu gcugucccuu      240

aacggaaugu gcccccuguc uaucgaggag gucgaagagu ggaaaaagag cggcuucaaa      300

gaauauccuc ucaauccgga guuucuggag uggcugcgca ccuccgacga ugaaaacauc      360

aaucccuacu ucuuuagaga caaggccagc aagcagaaag ugacacucuu cgagguuggc      420

cgggccuuuu aucacauugc ucaacgcaga ggguuccugu cuaaccggcu ugaucagucc      480

gccgaaggaa uccuggagga acauuguccu aagauugagg cucucgugga ggaccugaau      540

agcaccgaug aaauccuuac aucucugaaa gacuacuuuu ucgauaccgg cauucucgac      600

gccaacgaga aagggggauu ugcgaaagau cuggacgaag gcgacaagaa acuggucucc      660

cucuaucgca gcauccaggc cauugugaag aagaaugaua cagacuucga gaaagggaag      720

gcugaaauca ucgagagacu gaacaaaaag gauguucuug gaaaagugaa gggcaagauu      780

aaagacaucu cucaggccau gcuggagggc aauuuuaaga cccucgggca auacuucuuc      840

ucccuguaca acaaagaacg gauucgcaau caguauacga gcagagagga ucacuaccuu      900

gaagaguuua agaucauuug caaauuccag ggaaucggcu ccaucaacca gaaugaaaag      960

cugcccgaga agaaauuuga ggggcucgcu aaggaacugu auaaagccau uuucuuucaa     1020

cggccucuga agucucagaa aggacucauc ggcaagugua gcuucgagaa guccaaaagc     1080

agaugcgcga uuucucaucc cgacuuugaa gagaagcgca ugugggagua ccugaacacc     1140

aucaaaauug ggacacaguc cgaaaagguc cuucgguucc ugagccagga ggaaaaacuc     1200

aaccugaucc cuaaguuucu uagaaagaag gauaauuuca acuuugagga ccuggccaag     1260

gaacucaucg agaaaggagc uaccuucggc uuuuauaagu cuagcaaacg cgauuccuuc     1320

cacuacuggu ucaauuauaa gccgacagac accgugucug gcugucccgu gagcgccucc     1380

cugaaaagcg ucguugggga ggauuggaag acgaagucuu uuauuuacaa aacaaccaac     1440

uccaagggaa ucgaagugag ccggacagug aauuacaaag accuguggca ucuccugucu     1500

gucuccacca gcgacauuua ucuuuacgag uaugcuaaga acaaacuggg ccucgaagau     1560

aagaaugcca aggccuucuc caaaaucaag cugaaaaagg acuuugggaa ccugucucuu     1620

aaugcuauua acaaaauccu cccuuacaug agagagggac ugcuguauag ccacgcggug     1680

uucauggcca auauugaauc caucguugau gaggugaucu ggaacgaccu cggccaacgc     1740

aaguacauuc aggauaagau cgcugccauu aucgccaaua acauugucga caaugggcug     1800

cuugaggugg uuaacggccu gaucaaagaa ugcaaggugg auggagcuua uuacucuaaa     1860

gaggccgcgg acaccuaccg gaaggaucuc agcaaaaagc uggucuccuu uuucaaauuu     1920

caccauaucg acgauggcau uaagcaggaa acaauccuga acgagcuuuu ccccguguuu     1980

auugaacaac ucaaggcuca cgaguucauc aaagucaaga gacuggacga gaaagugcug     2040

gaauuucuca agggggagaa ucaggaugga gaaguuuucu guaacaaugc caaacagcug     2100

aagaaacuuu aucauccuag cgacauugag guguuuaaaa agcagaucgu caaagacaag     2160

gauggcacgg agaccgggca acugauccuc ggaucucccc ugacauccag cauuaaaaac     2220

ccgauggcca ugcgcgcucu gcaccagcuu cggaaggugc ucaauacccu gaucguugaa     2280

gaacaggugg acucuuccac acgcauucac aucgagaugg ccagagagcu gaacgaugcu     2340

aauaagcgga aaggcauuca ggacuaccaa aacgaaaaua agaaagagag agagaaguau     2400

cgggaugaaa ucaaaaagcu cuaccuggcc gagugcaaca aggaaaucga gccuaccguc     2460

gacgaucuuc ugcgcuauca gcucuggauu gaacaggaca gaaaagagau cuacgagaau     2520

gggaacagca uuucuaucug ugauauuauc ggcgacaauc ccgcguauga uauugaacau     2580

acggugccuc ggagcaucuc ccaggacaac agccaaauga auaagacacu gugcucucag     2640

aaauucaaca gagaugugaa gaaacagaag augcccuucg agcugggaaa ccaccuugaa     2700

auccuccccc gcauugccca uuggaaggag gaagcugaca aucugucccg ggagaucgag     2760

augauuauca gaagcaccaa aaccgccgcu acaaaggaag ccaaagacaa gaaaauucgc     2820

agacggcacu accugacccu ccgcagagau uaccugcagg gcaaguauga ccgguuuauc     2880

uggacagagc cuaaaguugg guucaagaac ucccaaaucc cggauacggg aauuaucacc     2940

cgcuacgcgc aggccuaucu uaagucuuac uuuaauagag ucgaaagcgu gaaaggcggg     3000

augguggcug aguuccggaa gauuugggga auccaggagu ccuaucaaga aaacggcaaa     3060

aaguacuuua aagagaagga ccgcucuaag cauacacacc acaccauuga ugccaucaca     3120

aucgcuugua ugaccaaaga caaguaugau guccuggccc augccuggac gcucgaagac     3180

aaagagcaga uuaccgaagc uagaaagauc guggcggaga gcaaacccug gaagacauuc     3240

aaggaggauc ugaaaaagau ugaagagggg auccugguuu cccacuacac cccugacaau     3300

gugaaaaagc agucuaaaaa gauuguccgg gugcgcggca gaaagcagua cguugccgaa     3360

aucgagcggg augugaacgg aaaagcuguc gccaagaaag acauuagcgg caaggugauc     3420

uauaaacuug acaaggaugg gaaaaagcug cccagacucc aacagggaga cacaauccgc     3480

ggcucccugc aucaggauag cauuuacggg gccaucaaga auccucucaa aaccgaagag     3540

auucgguaug uuaucagaaa ggaccuggag ucuauuaagg cuuccgaugu ggacaacauc     3600

gucgaugaaa uggugaagga gaaagugaag gaagccaucg cgagcaaggu ccuuauucug     3660

ucuuccaauc cccagcagaa aaacaagauc gacggaaaag uuuggaugaa ugaggaaaag     3720

ggcauuccga ucaacaaagu gcgccucuac gcuaauagcg ugaagaaccc ucuggagauu     3780

aaggagcacu cuucccugag caaaucccgg cacgaacaua agcaaaaagu cuaugggcag     3840

aaugaugaga acuuugccau ggccaucuac gaaggccuug acaagaaagg aaagacggag     3900

agagcuuucg agcugaucaa cuauauggau gccggcuacu acuauaagcu caaaaauaag     3960

aacgaacgca aaaaggacau uaucagccug gugcccgaga aacacauuga aacaggguuu     4020

aaucuccugc ggucuaacaa gaucccuauu auccuuaaaa agggacugaa uguuauucuc     4080

uacaagaacg agaaagaaga gaucgauuuc aauaacccca auagcaucau uaagagacug     4140

uauaaaaucu ccggccugga caaggacggg auuaaacuua uguacugcau cucugcgaag     4200

ggaagcaccg agaacauugc uuauaugaau gaugugauca acaagcagaa acuguuugaa     4260

cucaaguccg agauggaaaa acugggccuc ugggacaagg ucuucucuga uuucaaaaau     4320

gacaaccuga aaaagagcga cuuuuucaag aaacuuaaug aucugcugaa caagcauuac     4380

uuugagaaua acgugugcga uuccaucggc ucuauuaaga aaguuaagau caaggaaagc     4440

gggcucacaa ccccuaaagg aggcgacgug auugauaacu ucaaggacuu ucccuauauc     4500

aaauucaagg ucuccaauuu uaacgcccug auugagggga ucgauuucaa aaucagcccg     4560

accggaaagu uugugcaaau uuga                                            4584


<210>  4603
<211>  4587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4603
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuguggag       60

caggauuuca agaauaaaca aggcaacauc auggggaugg gauccagagu cauucccaug      120

ucucaggaaa uccugggcga guuugcucgg gggaauacaa agagcgaaac ggccgagcgc      180

accagacucc gguccguucg cagacugcgg gaacgccacc uucugagacg ggagcgccuc      240

cauagagugc ugcacauucu gggauuccuc ccuaaacauu acgugaacca gaucgacuuu      300

caggagcggc ugggcaaguu cuuugaagag ucugaaccga agcuggcgua ugauacaggc      360

gacuuccugu uuaaacccag cuuccucgag augcuggagg aauuuaagau ccaccaaccu      420

uccuucgucg agggggauaa aaaggugccc uacgacugga ccauuuauuu ucuucgcaaa      480

aaggcucuga cagaaagaau cgagaaggag gaacucgccu ggguuauucu gucuuucaau      540

cagaaacggg gauacuauca gcugcgcggc gaggaugaag aggaaaagcc uaacaaacuc      600

gaggaauuuu acagccugaa gaucguggac auuaaagucg augagucccc caauaagaaa      660

ggggacaccu gguauagccu uacacuggag aacggaugga ucuacagacg gucuuccaag      720

gugccucucg augacuggcu gggcaagacc agagauuuca uuguuacgac cgacaucgac      780

gaagagggga auccgaaacu ugauaaggaa ggaaacccca aacgcagcuu ccgggccccu      840

ucugaggacg auuggggccu gagaaagaaa aagacagaaa aggagauccu cgacuccagc      900

aaaaccgugg gccauuuuau uuucgagaau cugcuggcug aucccacaca gaagaucaaa      960

gggcgccucg uccggaccau ugaaagaaag uuuuacaaag acgagcugaa gcaaauccuu     1020

gugucucaga accgcuucca cccugaacug uccgaugccg acaugauggc ugauugcgug     1080

cgggagcucu auaagcacaa ucauagccac cagaacuuuc uggccuccaa agacauuaag     1140

caucuucugg ucgaugacau caucuucuac cagagacccc uccgcucuca aaagagcacg     1200

auuggaaauu guccgcugga guuuaagaca uucaaaaagg acggcaucga aauuaagguu     1260

ccuuauaaag uggcgcccaa gucccacccu cuguuucagg aguuccggcu ccugcagugg     1320

gugagcucuc uuagaaucau ucgcaaaucc gaugacagcg augucacccg ggaauuucug     1380

gggacacaag acaagaccaa acagcuccug gauuaccuga ugacccaguc ugagauggac     1440

ugcaacaaac uuaugacauu ccuccugacg caggaaggau auaaaagaaa ggaucuuaag     1500

agcgaggugg gcaaauuucg guggaacuac guuuucgacu ccgagaccgg ggaaucuaag     1560

acauaucccg gaaauagcac ccgcgccgag uuccucaaaa gacugggcaa gguggaaggc     1620

uuugauuucu ccaaauacac agaggaauuu gaggagagcc uuuggcaccu ggucuacucu     1680

gugaccgaca agguugaguu cgaaaaggcu cuccggaaau uuuccagaaa gaacgggauc     1740

gaaaucaaua gcuucgugga gaaccugaaa aaguuucccc cuuauaaauc ugaauacgcc     1800

uccuauagcc ugaaggcuau uaagaaacuu cucgcccuga ugcgcuucgg agagaauugg     1860

aaggaggauu ccaucgacuc uauuagcaaa caucggaucg aaaagauucu gacgggcgag     1920

uacgauccgg aaaucaaaga caaggucaga gagauuuccc gcgcguuuga cgagguggaa     1980

gauuuccaag ggcuccccgu cugguuugcc aacuaugugg uuuacggaag acacucugag     2040

gcuagcgaac ugaucaagug gacauccagc gacgagaugg ccgauuucau caaagaauuu     2100

aagcagcauu cucuucggaa uccuauugug gagcaggucg ugcuggagac ccuccgcguu     2160

gugaaagaca ucugggauaa guauggccac gggcagaaag acuucuucga ugaaauucau     2220

gucgagcugg gcagagaacu gaagaacccc gcugagcaac gggcccgccu uaccacacag     2280

aucuccgcca augaaaacac caaucucaga auuaaaagcc ugcuggugga gcuccugcac     2340

gacccuggag uggagaacgu ucggcccuac ucuccgaugc agcaggaaau ccuuaagauc     2400

uacgaggaag gcguccugaa guccagcuuu gaggugccug augacauucu caaaaucucu     2460

aagacagcuc aacccagcuc cagcgaucug aaacgcuaua agcuguggcu ugaacagaaa     2520

uacagaucuc cuuauacggg gaagauuauc ccccucaaua agcuguucac cccugcguac     2580

gagguggagc acgucauucc ccagucccgg uauuuugacg auagcuucuc caacaaagug     2640

aucugugaag ccgcuguuaa uaagcugaaa gacaaccgcc ucggaaugga guuuaucaag     2700

ugcuuccaug gccagagagu gucucugggg uuuggagaag aggucgagau uuucagcgaa     2760

uccgccuaca aagccuuuau ccaagacaac uucccgaaga auccugcuaa gaaacggaaa     2820

cuucugaugg augagcuccc cgaacagaug guggagagac agaugaacga cacacgcuau     2880

auuucuaagu uuguuaccag cauccugucc aauauugugc ggucugaaga gaacgauaca     2940

ggcuucaaua gcaagaacau cauccccggg aauggcaaaa uuaccacggc ccugaagcaa     3000

gacuggggac uuaacgaugu cuggaaugag cugauccucc cuagauucga acgcaugaac     3060

cagcugaccg gcacaaccgu guuuacagcg uggaaugaga accaccagaa guuccucccg     3120

accguucccc uggaauacgc uaaaggguuu cagaagaaac ggauugacca uagacaccac     3180

gccauggaug cccuugugau cgcuugugcc acgcgcgacc augucaauua ccugaacaau     3240

gagcacgcuc ucggaaagaa cuauaaaggc aaggaagaga agcaaucccg gagauucgau     3300

cugcgccaga aacugugccu uaagaaaaag aacgggcaua gccaggagca guacgaaugg     3360

aucuuuucua aaccuuggga cuccuucaca caagaugcca agaaaaaucu ggagcggauc     3420

auugugagcu uuaagcagaa ccucagagug aucaauaaga ccucuaacaa auaucugucc     3480

uacaaggacg aagagggaaa ucuccggcug gacagccagg gcaaacccgu caaggcgcuu     3540

acacgccaga ucaaagggga ugccugggcu auuagaaagu cuauccacaa ggacaccguu     3600

uccggccugg ugaaacuccg gcgcgugaag auggucaacc ugagcguggc ccuggauaau     3660

ccugaaaaca uucuuaauaa aaagcugaaa aagaagaucg cugagcucgu uaaacugaac     3720

uaugacaaua agcuccugcu uaaauacuuc agagaucggg acuauauguu ugauggaaga     3780

gaggugucca agcuggacgu cuacuacuuc gaagagggcc uggccgcgag ccgcgugucu     3840

cucgauacca gcuuuuccga caaaaagauu caaucuauca cagauacggg gauccggaag     3900

auucugcuug cccaucuggc uaacuaucac ggaagacucg acgaaaaagg caagguuauu     3960

cccccugaga cccuggccuu cacacccgag gggauugagg aacucaauaa aaacaucaag     4020

ggacugaaug gcgggaaauu ucaccagccg auuuucaagg ugcgcaccua cgaaccuaaa     4080

ggcaacaagu uccaggucgg auuuaagggc aacaaaaaua gcaaguaugu ggaggcugcc     4140

aaagggacaa accuuuucuu ugccaucuac cugaaugaug aaggaaagcg guccuaugag     4200

accauccccu ucaacguugu gauggagaga cugaaacaag gcgaagggcc ugucccccag     4260

acgaaugaga agggaggcaa gcuccuguuu caucuuucuc cgaacgaacu gguguacgug     4320

ccugagacag acgaaaauua uaccaacguc gacuucaaaa aucucagcaa ggagcagguu     4380

gagaaaauuu acaagauggu guccucuacc ggcagcgaau gucacuuugu gcgcgcugau     4440

aucuccagcc ugauuaaagg guacgacgcg aagacaaagg ucggagagcu cggcucucug     4500

aacaaacagg aagugaccaa uaacgggaug acacggauca aggagaaaug cuggaagauu     4560

aaaaucgaua gacuuggauc cauuuga                                         4587


<210>  4604
<211>  4587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4604
augaagaaaa uccugggcau ugaccucggg accaacagca ucggaugggc cguggucaau       60

uccuucgaug agaaccagaa ugaagagucu cugaagcaca uugaaugcgu uggcagcaga      120

aucauuccca uggacgcugc cauccuuggg gauuuugaca aaggaaacuc caucucucaa      180

acagcgaagc ggacggaguu ccgcagcgug agacaucugc gggaacgcca ccuccugaga      240

cgggcucgcc uucauaaaau ucugucccuc cugggcuuuc ugccugauca cuacgugcag      300

gaccucgauu cucaugggaa uuucccgguc aacguggagc ccaagaucgc cuggagaaag      360

gacagcaaug gagccuuuga guucauuuuu aacgauuccu ucgacgaaau gcugcaggau      420

uuugcuaccu aucagccuga gcuuaucgcc uguaauaaaa agguucccua cgacuggaca      480

auuuauuacc ugcggaaaaa ggcucucacc aaaaagcuga gcaaggagga gcuuucuugg      540

auccugcuca acuucaauca aaaacgcggc uauuaccagc ugagaggcga ggaagaugac      600

gaguccguca agagcgccaa aacacggcag uauuuugacc gccagaagau uaaaucuauc      660

guggauacca accaaacaua caaggggcag aaagugcuga ucguugaacu cgaggacgga      720

auuaagggca agaucuucaa aaaggaaauu ccuaauuggg uggggcagga gaaauccauc      780

auugucacca ucgauaucga caaggaugga ugcgacaaau acgaggaaag cggcgagcug      840

ucuuguagau uuaagauucc cuccgaugcg gaaugggaga gcgaauggaa acuuaucaaa      900

augaagacgc agaaagaccu ggaugccucc gggaagaccg ugggagcuua uauuuacgac      960

gccauccucc aaaacccuuc ucagaaaauu cggggcaagc uggugcgcac aaucgagaga     1020

aaguauuaca aagaggaacu uaagcagauc cugaaaaagc aagucgaguu ccacccggaa     1080

cuccgggaua aagaccuguu ccuggacugc cucaaugagc uguaucccaa caacgaugcu     1140

cacagaaaug ccugugcgga ccgcgauuuu auuuaccuuu ucauggacga uauccuguuu     1200

uaucagcggc cucucaagag caagaaaucc cugauugccg acugccccua cgaaaagcau     1260

auguacagag auaaagagac cggcgagcuu aaguauagcu cuguuaaaug uaucgcuaag     1320

ucccacccuc uguuccagga auuucgccuc uggcauuuca uuagcaacau ccggauuuac     1380

cagaaagaga gaguggugga cgucgaugcc gaccaaucug cucugagcuc cgcccgcucu     1440

gcccucaaga gcaagcugga agcugaugug gacguuacaa augaguuucu gaccugcgag     1500

gcggauuaug ugucccuuuu cgaauggcug aacaauaaaa agagcgucuc ucagaaagac     1560

uuucucaagu accccgccuu cgggaucaaa aaguccggag acaacuaucg guggaauuac     1620

guggaggaua agagcuaucc guguaacgaa acgagagcuc ugauccuguc ucaccuugag     1680

aaagccaaua uucgcggcga caucuuuaca cgggaacagg aggaggcccu cuggcacauu     1740

cuguacuccg uugaagauag auacgagauc gugaaggcuc ugcagagcuu cgccgcgaaa     1800

uaucgcuuua acgacgauuu cgucaccgug uucuccaagu uuccucccuu caaaaaggaa     1860

uacgggucuu auagcgcuaa ggccauuaaa aagcuccugc cucuuaugcg gaugggaaaa     1920

uacugguccg aggccgcuau cgacaucaag acaagagaac ggauugagaa aaucauuacc     1980

ggcgagugcg augacucuau ccgcgaaaga guccggcuga aggccauuag acuccaugau     2040

aucggggacu uucgcgcucu gcccgugugg cuggccuguu auguugugua cgaucggcac     2100

agcgaguccg gagacaucac caaguggaaa acaccccaag aaauggauug guauauucag     2160

aauuucaagc agcauagccu uagaaacccu aucgucgaac aggugauuac ggaaacccuc     2220

cgcguuguga gagacaucug gaaacaaguc ggccggauug acgagaucca cguggagcug     2280

ggccgcgaaa ugaagaaucc ggcggauaaa agagccaaga ugacagcucg gaugaucgag     2340

aacgaaaaua ccaaccugcg cauuaaagcc augcucacag aguuucugaa ccccgaguuc     2400

gaaaucgaga augugagacc uuacucugcu ucccagcagg acauucuucg gaucuacgaa     2460

aacgggguuc ugaauagcgu cucugagauu cccgaagaga ucaccggcau ucucaagaag     2520

uuuaacgagc gcgaacagag aaaacggccu uccacgagcg aggugcugcg cuauaagcug     2580

uggcuugaac aaaaauacag aucucccuau acaggaaaga ugaucccgcu cgggaaacug     2640

uucaccccug ccuacgagau cgaacauauu aucccccaga gccgguauuu ugaugacucc     2700

uucaauaaca aggugauuug cgaggcggag gucaauaagc ugaaaggaaa ccuccugggc     2760

uacgaauuua ucaagaaaca ccacgggaga cgcguggauc uuggccuggg agagauuguu     2820

gacaucuuca gcguggauga auaugagacc uuugucaagg acuacuacuc ugccaaucag     2880

caaaagauga agaagcuccu gauggaugaa aucccugagc aguucauuga gcggcagcug     2940

aacgacucca gauauaucag caaagugguu aagucccuuc ucucuaauau ugugcgcgaa     3000

caggaugagc aagaaagcau cuccaaaaac cugauugucu gcacaggcgg ggugaccgac     3060

cggcugaaga aagacugggg aaucaaugau guuuggaaca agcucauccu gcccagauuc     3120

cagcgccuua auaacauuac agagacgacc gaguuuacag cuaagaacau gcagggccau     3180

gaaaucccug ugguccccuu cgaguaccag cggggguuua auaaaaagag aauugaccac     3240

cgccaucacg ccauggaugc uaucgugauu gccugugccg gacggaacau cgugaauuau     3300

cugaacaaug aaucugcucg gaaaaaggcg caaauuagca gauacgaccu ccagcgccug     3360

cugugcgaua aagucaagac cgacgauaac ggcaauuaua aguggauccu uaaaaagccg     3420

ugguccacac ugacccagga cguuuacgcc gcucucgaua acgugaucgu gucuuucaaa     3480

cagaaucuga gagucauuaa caagacgaau aaccacuaug ugcauuacga ggccgggaaa     3540

aagaucauug ccaaacaaga aaaggcuagc ugggccaucc ggaagccuau gcacaaagac     3600

accguuuuug gcgaggugaa ucuccgcaag gucaaaacag ugucccugaa cgaggcgauu     3660

aagagccccc auuguaucgu ucagaaagau uucaagaaga aacuucuguc ucuccugaag     3720

cagaauuuug acgcuaaaga agugaaggcc uacuucgagg aacacaaaga ugucuggucc     3780

gacaucaacc ugagcaagau ugagguguau uacuuuacca aggacacaaa agaaagauuc     3840

uuugccaccc ggaagccccu ugauucuucc uucgaccgca aaaagaucga gagcguuacg     3900

gauacaggca uucagaaaau ccugcucaga caucuggagc aaaagaacgg agacucugau     3960

cucgcuuucu ccccugacgg gauugaagag augaaucaga acaucgugca gcugaaugga     4020

ggccggaagc accaaccgau cgucaaagug agaguguaug aaaaggccga uaaauuugcg     4080

auugggcagc agggaaacaa ggcuagcaaa uacgucgagg ccgccaaggg caccaaucuu     4140

uucuuugcug uuuaugaaga ggaggugcgc gacaaaaaga caaacgccgu gauguucaag     4200

cggaauuacg cuuccauccc ccugaacguc gccauugaua gacagaaaaa ggggcucagc     4260

accgcgccug aagacgagaa uggcaacgcc cccaucuuug ugcugucucc uaaugaucug     4320

guuuaucuuc ccaccgcuga agacauuauc aacgcccgca uuagcuuccc uaucaauaaa     4380

aaccggaucu acaagauggu guccugcaca ggauaccaau gcuuuuucau uaaacaccau     4440

gucgcuaaug ugaucguuga uaagaaggag uuuucugccc ugaacaaaau ggagaaggcc     4500

gaaacgaaug agaugauuaa agacaucugu gugcccauua agcucgacag acugggcaac     4560

aucgucaaaa ucggggugua uaaguga                                         4587


<210>  4605
<211>  4587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4605
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cguggucaau       60

uccuucgaug agaaccagaa ugaagagucu cuuaagcaca ucgaaugcgu uggcagcaga      120

auuaucccca uggacgcugc cauucugggg gauuuugaca aaggaaacuc caucucucaa      180

acagcgaagc ggacggaguu ccgcagcgug agacaucucc gggaacgcca ucugcuuaga      240

cgggcucgcc ugcacaaaau ccucucccug cugggcuuuc ucccugauca cuacgugcag      300

gaccuggauu cucaugggaa uuucccgguc aacguggagc ccaagauugc cuggagaaag      360

gacagcaaug gagccuuuga guucaucuuu aacgauuccu ucgacgaaau gcuucaggau      420

uuugcuaccu aucagccuga gcugauugcc uguaauaaaa agguucccua cgacuggaca      480

aucuauuacc uccggaaaaa ggcucugacc aaaaagcuga gcaaggagga gcugucuugg      540

auucuccuga acuucaauca aaaacgcggc uauuaccagc ugagaggcga ggaagaugac      600

gaguccguga aguccgccaa aacacggcag uauuuugacc gccagaagau caaaucuauu      660

gucgauacca accaaacaua caaggggcag aaagugcuca ucguugaacu ggaggacgga      720

aucaagggca agauuuucaa aaaggaaauc ccuaauuggg uggggcagga gaaauccauu      780

aucgucacca uugauaucga caaggaugga ugcgacaaau acgaggaaag cggcgagcuu      840

ucuuguagau uuaagauccc cuccgaugcg gaaugggaga gcgaauggaa gcugauuaaa      900

augaagacgc agaaagaccu cgaugccucc gggaagaccg ugggagcuua uaucuacgac      960

gccauucugc aaaacccuuc ucagaaaauc cggggcaaac uugugcgcac aauugagaga     1020

aaguauuaca aagaggaacu gaagcagauc cucaaaaagc aagucgaguu ccacccggaa     1080

cugcgggaua aagaccuguu ccucgacugc cugaaugagc uuuaucccaa caacgaugcu     1140

cacagaaaug ccugugcgga ccgcgauuuu aucuaccugu ucauggacga uauucucuuu     1200

uaucagcggc cucuuaagag caagaaaucc cuuaucgccg acugccccua cgaaaagcau     1260

auguacagag auaaagagac cggcgagcug aaguauagcu cuguuaaaug uauugcuaag     1320

ucccacccuc ucuuccagga auuucgccug uggcauuuca ucagcaacau ucggaucuac     1380

cagaaagaga gaguggugga cgucgaugcc ugccaacccg accugucuag cgcucgcucc     1440

gcccucaagu cuaagcugga agccgaugug gacguuacaa augaguuucu uaccugugag     1500

gcugauuaug ugagccuguu cgaauggcuc aacaauaaaa aguccgucag ccagaaagac     1560

uuucugaagu acccggcguu cgggauuaaa aagucuggag acaacuaucg guggaauuac     1620

guggaggaua aguccuaucc uugcaacgaa acgagagccc ugauccuuag ccaccucgag     1680

aaagcuaaua uccgcggcga cauuuuuaca cgggaacagg aggaggcccu guggcacauc     1740

cuguacucug uugaagauag auacgagauu gugaaggccc uccaguccuu cgcugccaaa     1800

uaucgcuuua acgacgauuu cgucaccgug uucagcaagu uucccccuuu caaaaaggaa     1860

uacggguccu auucugcgaa ggcuaucaaa aagcugcuuc cccugaugcg gaugggaaaa     1920

uacuggagcg aggccgccau ugacaucaag acaagagaac ggaucgagaa aauuaucacc     1980

ggcgagugug augacuccau ucgcgaaaga guccggcuca aggcuaucag acugcaugau     2040

auuggggacu uucgcgcccu gcccgugugg cuugcuugcu auguugugua cgaucggcac     2100

ucugagagcg gagacaucac caaguggaaa acaccucaag aaauggauug guauauccag     2160

aauuucaagc agcauucccu cagaaacccg auugucgaac aggugaucac ggaaacccug     2220

cgcguuguga gagacauuug gaaacaaguc ggccggaucg acgagauuca cguggagcug     2280

ggccgcgaaa ugaagaaucc cgccgauaaa agagcgaaga ugacagcccg gaugaucgag     2340

aacgaaaaua ccaaccuccg caucaaagcu augcugacag aguuucuuaa cccugaguuc     2400

aagauugaaa augugagacc cuacagcgcc ucucagcagg acauccugcg gauuuacgag     2460

aacgggguuc ucaauuccgu cagcgaaauc ccugaggaaa uuaccggaau ccugaagaaa     2520

uuuaacgagc gcgagcagag aaagcggccc ucuacguccg aagugcugcg cuauaaacuu     2580

uggcucgagc aaaaguacag aagcccguau acaggcaaaa ugauuccucu ggggaagcug     2640

uucacccccg cuuacgaaau cgagcauauc auuccucagu cucgguauuu ugaugacagc     2700

uucaauaaca aggugaucug cgaagccgag gucaauaaac ucaagggaaa ccugcuuggc     2760

uacgaguuua uuaaaaagca ccacgggaga cgcguggauc ugggccucgg agaaaucguu     2820

gacauuuucu ccguggauga guaugaaacc uuugucaaag acuacuacag cgcgaaucag     2880

caaaagauga agaaacugcu gauggaugag auccccgaac aguucaucga gcggcagcuu     2940

aacgacucua gauauauuuc caaggugguu aaaagccucc uguccaauau cgugcgcgag     3000

caggaugaac aagagucuau uagcaagaac cugaucgucu guacaggcgg ggugaccgac     3060

cggcucaaaa aggacugggg aauuaaugau guuuggaaca agcugauccu uccuagauuc     3120

cagcgccuga auaacaucac agaaacgacc gaguuuacag ccaaaaacau gcagggccau     3180

gagauucccg uggucccguu cgaauaccag cggggguuua auaagaaaag aaucgaccac     3240

cgccaucacg cuauggaugc cauugugauc gcuugcgccg gacggaacau ugugaauuau     3300

cucaacaaug aguccgccag aaagaaagcu caaaucucuc gguacgaccu gcagcgccug     3360

cuuugugaua aggucaagac cgacgauaac ggcaauuaua aauggauucu gaagaaaccu     3420

uggagcacac ucacccagga cguuuacgcg gcccuggaua acgugaucgu guccuucaag     3480

cagaaucuca gagucaucaa caaaacgaau aaccacuaug ugcauuacga agcugggaag     3540

aaaauuaucg ccaagcaaga gaaggccucu ugggcuauuc ggaaacccau gcacaaggac     3600

accguuuuug gcgaagugaa ucugcgcaaa gucaagacag ugagccuuaa cgaggccauc     3660

aaaagccccc auugcauugu ucagaaggau uucaagaaaa agcugcucag ccugcugaaa     3720

cagaauuuug acgcgaagga ggugaaggcu uacuucgagg aacacaagga ugucuggucu     3780

gacaucaacc uuuccaagau cgaaguguau uacuuuacca aagacacaaa ggagagauuc     3840

uuugccaccc ggaaaccucu ggauagcucu uucgacagaa agaaaauuga auccguuacg     3900

gauacaggaa uccaaaagau ucuccugcgc caucucgagc agaagaacgg cgacagcgau     3960

cuggccuucu cuccggacgg gaucgaggaa augaaucaga acauugugca gcuuaaugga     4020

ggccggaaac accaacccau cgucaaggug agaguguaug agaaagcuga uaaguuugcc     4080

aucgggcagc agggaaacaa agcguccaag uacgucgaag cugccaaagg caccaaucug     4140

uucuuugccg uuuaugagga agaggugcgc gacaagaaga caaacgcugu gauguucaaa     4200

cggaauuacg ccagcauucc ucucaacguc gcuaucgaua gacagaagaa agggcugucc     4260

accgcccccg aggacgaaaa uggcaacgcg ccuauuuuug ugcugagccc caaugaucuu     4320

guuuaucugc cuaccgccga ggacaucauu aacgcucgca ucucuuuccc cauuaauaag     4380

aaccggaucu acaaaauggu gagcuguaca ggauaccaau gcuuuuucau caagcaccau     4440

gucgccaaug ugauuguuga uaagaaagaa uuuuccgcuc ucaacaagau ggagaaagcc     4500

gagacgaaug aaaugaucaa ggacauuugu gugccgauca aacuggacag acucggcaac     4560

auugucaaga ucggggugua uaaguga                                         4587


<210>  4606
<211>  4590
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4606
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc cguggucaau       60

uccuucgaug agaaccagaa ugaagagucu cuuaagcaca ucgaaugccu gggcagcaga      120

auuaucccca uggacgcugc cauucucggg gauuuugaca aaggaaacuc caucucucaa      180

acagcgaagc ggacggaguu ccgcggcguu agacaucugc gggaacgcca ccuucugaga      240

cgggcucgcc uccauaaaau ccugagacug cucggguuuc ugccugauca cuacgugcag      300

gaccuugaug gacauggcaa uuuccuggug aacgucgagc cgaagauugc cuggcggaag      360

gacagcaaug gcaccuuuga guucaucuuu aacgauuccu ucgacgaaau gcuccaggau      420

uuugccacau aucagcccga gcugauugcu uguaaucgca aagugccuua cgacuggacc      480

aucuauuacc uuagaaagaa agcccugaca aagaaacucu cuaaggagga acugagcugg      540

auucugcuca acuucaauca aaagcggggg uauuaccagc ugcgcggaga ggaagaugac      600

gaguccguua aaaccgcuaa gacaagacag uauuuugacc ggcagaaaau caagagcauu      660

guggauacca accaaacgua caaaggccag aagguccuua ucguggaacu ggaggacggg      720

guuaaaggaa agaucuucaa gaaagaaauu cccaauuggg ugggccagga gaagucuauc      780

auugucaccg uggauaucga caaagauggg ugcgacaagu acgaggaauc cggagagcuc      840

agcugucgcu uuaaaauucc uucugaugcc gaaugggagu ccgaauggaa gcugaucaag      900

augaaaacac agaaggaccu ugaugcgagc ggcaaaaccg ugggcgccua uaucuacgac      960

gcuauucugc aaaaccccuc ucagaagauc agagggaaac ucguccggac aauugagaga     1020

aaguauuaca aggaggaacu gaaacagauc cugaagaaac aaguugaguu ccacccugaa     1080

cuccgcgaua aggaccuguu ccuugacugc cugaaugagc ucuauccgaa caacgaugcc     1140

caccggaaug cuugugccga cagagauuuu auuuaccugu ucauggacga uauccuuuuc     1200

uaucagcgcc cccugaaauc caaaaagagc cucaucgcgg acugcccuua cgaaaaacau     1260

auguaccggg auaaggagac cggagagcug aaauauuccu cugugaagug uauugccaaa     1320

agccaccccc uguuccagga auuuagacuc uggcauuuca ucuccaacau ucgcaucuac     1380

cagaaggagc gggugauuga cgucgaugcu ugccaaccug accugagcuc ugccagaucc     1440

gcucuuaaaa gcaagcugga agccgaugug gacguuacga augaguuucu cacaugugag     1500

gccgauuaug ugucucuguu cgaauggcug aacaauaaga aaagcgucuc ccagaaggac     1560

uuucuuaaau accccgcuuu cggcaucaag aaaucugggg acaacuaucg cuggaauuac     1620

guggaggaua agagcuaucc gugcaacgaa acccgggcgc ucauucuguc ccaccuggag     1680

aaggccaaua ucagaggaga caucuuuaca cgcgaacagg aggaggcucu cuggcacauu     1740

cuguacagcg uugaagaucg guacgagauc gugaaagccc uucagucuuu cgccgcuaag     1800

uauagauuua acgacgauuu cgucaccgug uucuccaaau uuccucccuu caagaaagaa     1860

uacggcagcu auucugccaa ggcgauuaag aaacugcucc cucugaugcg gauggggaag     1920

uacugguccg agagcgcuau cgacauuaaa acccgcgaaa gaaucgagaa gaucauuaca     1980

ggagagugug augacuccau ccgggaaaga guccgccuga aagccauucg gcuucaugau     2040

aucgagcacu uuagagcccu ccccgugugg cuggcuugcu auguugugua cgaccgccau     2100

ucugaaagcg gcgauauuau gaaguggaag acgccccaag agauggacuc cuauauccag     2160

aauuucaaac agcacucucu gagaaacccu aucgucgaac aggugauuac cgagacacuc     2220

cggguugugc gcgauaucug gaagcaaguc ggcagaauug acgagaucca uguggaacug     2280

gggcgggaga ugaaaaaucc ggccgacaag cgcaaagaaa ugaccaagag aauuaucgag     2340

aacgagaaua caaaccuucg gaucaaagcu augcugaccg aauuucucaa ccccgaguuc     2400

gaaauugaga augugcgccc uuacagcgcc ucccagcagg auauccugag aauuuacgaa     2460

aacggaguuc ugaauagcgu cucugagauc cccgaggaaa uuacgggcau ccuuaagaag     2520

uuuaacgagc gggaacagcg caaaagaccu uccacaagcg aggugcuccg guauaagcug     2580

uggcuggaac aaaaauacug cucucccuau accgggaaga ugauuccgcu cggaaaacug     2640

uucaccccug cguacgagau cgagcacgug aucccccagu ccagauauuu ugacgauagc     2700

uucaauaaca aggucauuug ugaagccgag gugaauaaac uuaaaggcaa ccugcucggg     2760

uacgaauuua ucaagaaaca ccauggccag cgcguugacc ugggacuggg cgagauugug     2820

gauaucuucu cugucgacga auaugagaca uuugugaagg auuacuacag cgggaaucaa     2880

cagaaaauga agaaacuucu cauggacgag auuccugaac aguucaucga gcggcagcug     2940

aacgauucca gauauaucag caagguugug aagucucugc ucuccaauau uguccgcgaa     3000

caagacgagc aggagagcau cuccaaaaac gugauuguuu gcaccggagg cgugacagau     3060

cggcugaaga aagacugggg gaucaaugac gucuggaaca aacuuauucu gcccagauuc     3120

cagcgccuca auaacaucac ggaaaccaca gaguuuaccg cuaagaacau ggaaggacac     3180

gagaucccug uggugcccuu cgaauaccag cggggcuuua auaagaagag aauugaucau     3240

cgccaccacg ccauggacgc uaucgucauu gccugugcgu cucggaacau cguuaauuau     3300

cugaacaaug agagcgccag aaagaaggcu cggauuuccc gcuacgaucu gcaaaaacuu     3360

cugugcgaca aggugagaac agaugacaac gggaauuaua aguggauccu caaaaagccg     3420

uggggcaccc ugacgcagga uguguacgcc gcucucgaca acgucauugu gucuuucaaa     3480

cagaaucugc ggguuaucaa caagaccaaa aauuauuacg ugcauuacga ggccggaaag     3540

aaaacaaucg ccaagcagga aaagggcagc uccugggcua uucgcaaacc uaugcacaag     3600

gauaccgucu uuggggaggu gaaccuuaga aaaguuaaga cagugucucu gaaugaagcg     3660

aucaaaaacc cccaauguau ugucgagaag gacuucaaga aaaagcuccu gagccugcuu     3720

aaacagaauu uugaugccaa gaaagugaag gcuuauuucg aggaacauaa ggacguuugg     3780

uccgauauca accugagcaa aauugaggug uacuauuuua ccaaggacac gaaagaacgg     3840

uucuuugcca caagaaagcc ccucgacucu uccuucgaua aaaaggagau cgaaagcguc     3900

accgacacag gaauccagaa gauucugcuc cgccaccugg agcaaaagaa cggcgauccu     3960

gaccuugccu ucucuccgga ugggaucgaa gagaugaauc auaacauugu gcagcugaau     4020

ggaggccgga agcaccagcc caucgugaaa gucagaguuu acgagaaggc ugacaaauuu     4080

gccauugggc agcaaggcaa caaguccagc aaauaugugg aagcggcuaa gggaaccaau     4140

cucuucuuug ccguguacga ggaagagguc cgcgauaaga aaaccaacgc cgugaugcug     4200

aagcggcacu augcuucuau cccucugaau guugccaucg acagacagaa aaagggccuu     4260

uccacagcgc ccgaggauga aaacgggaau gugccuauuu ucguccugag ccccaacgac     4320

cucguguacc ugccuacggc ugaggauauc gccaaugccu ccauuagcuu ucccaucaac     4380

aaaaaucgca uuuacaagau gguuucuugc accggaaacc gguguuucuu uaucaagcau     4440

cacguggcua augucauugu ggacaaaagc gaauuuuccg uccucaacaa gaucgagaaa     4500

gccgaaacag augagaugau caaggagauu ugcgugccga ucaaaauuga cagacugggc     4560

aauaucguua agauuggggu guauaaguga                                      4590


<210>  4607
<211>  4608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4607
augaacacca uccugggccu cgaccugggg acaaauagca uuggaugggc ccuuaucaag       60

gugcugaacg auaaaacgag acucccccug gagauuaucg accuuggcgu cguugugauu      120

ccuaccgauc agcggacacu ggacaaguuc uccaaagggg aaucuaucag caagaccgcu      180

gagcgcacaa aacaaaaggg agugagaaag cucuacgaac ggaaaaagcu gcgcagagag      240

cggcugcacc gcguccucaa uauccugggc uuucuuccgg aacauuauuc caaaaagauu      300

gauuucaaaa agcaccuggg gcaguuuuuc ggaaacucuc agcccaagau cagcuacgug      360

gagaaugacc auggcaaaca cgaguuuauu uucauggauu ccuauagaga aaugcucaag      420

gaguuuucua aacauaacau caagaauguu ccuuacgacu ggaccauuua uuaccugcgg      480

aaaaaggccc uuagccagaa aaucuccaag gaggaacugg cgugggugau ucucaacuuc      540

aauaagaaac gcggcuauua ccaauuuaga gaugacaacg cugaugagaa uaaggaaaac      600

aaaaucgaga aguacuaugc ccugaaaguc acaaauguga agaaggaaga cgcccagaac      660

uacaccauca cgcuggagaa uggguggauu uauaaaaaca agagcaaaau cucucuccug      720

gauuggguug gauccaccaa ggaguucauu agcacagaaa aauuugacaa caagggcaau      780

aucauuaaga aaaagaacgg ggaggacgau cggacccuua aacuggugga cucugaaaag      840

gauuggaucg cuaucaaaaa guccacagag gaccgcauug aaaauuacgg aaagaccguc      900

ggcacguauc ucuacgagca ucugcuuaaa gaucccaacc agaagcugaa uggggaacuc      960

aucaacauua ucgacagaaa auucuauaag aaugagcuga aacagauucu ggagaagcaa     1020

aaagaauuuc acccugagcu ccugaacgaa gaucuuuucc aaaagagcau caucgagcug     1080

uaccccaaaa auucuacacg gcagaaagaa cucaagaaca aagaccugug cuaccuucug     1140

auugaugacg ugaucuauua ccagcgcgau cucaagaaaa agaacuccac cauuaauaag     1200

uguagcuucg aguccagaga guauuaccug aauggagaaa aacagaucca gaagauuaac     1260

ugcaucgcca aaucuaagcc ucuguaucag gaguuucgga cacuccaauu cauuaaaaau     1320

cugaaggugu acgaaaucau cgacggccuu gagaaggaca uuaccagcaa acugcucccg     1380

gauuuugaga ccaaggaaca cuucuuugag uggcugaacg acaaagaaaa gaucgaucag     1440

cugcagcuuc ucuccuauaa auguuucgac cugcacgaua acgucaagaa ugaccugauu     1500

gcuaagggca ucaaaagacc cacagagaag gccauucgca cggaacuccu ggagaaauac     1560

cgguggaacu acagcaagga uaaugacaga aacuuucuuc ugaaugagac ccgcuucaaa     1620

auccucaagu aucugaaaaa gguugauaag aaacugacac uuaccaacga acagaucgag     1680

ucucucuggc ugcugcucua cuccgcgaag gacgaacggg agcugaaaaa ugggcuucaa     1740

aaguuuagcg aaaagaacaa gcugccuaag aaauucucug auauuuuuau cagcuuuccc     1800

ccuuucaaga aagaguaugg agccuacucc gagaaggcuc ucaaaaagcu gcugucucuu     1860

gugagauucg gcaaguauug gagcuucaau aaaauugaca acaauaucca gaacaagauu     1920

augaauaucc ucaacgggga aguggaugac uccaucagcg accgcguccg ggagaaaaca     1980

auucugcugc aggauaagaa ugacuuucag gaucuccccc uguggcuugc cucuuacgug     2040

gcuuauaaca gauucuccga aguuagcgag aucaaauacu gggacucucc ggauaagauu     2100

gaauuucuga agcaacauuc caucggaaac ccuauugugg agaaaaucau caaugagacc     2160

cucaagcuga uuaaagacau cugggucauu cacggcaaga gcugcaaaaa cuucuuuucc     2220

gaaauccaca uugagcuguc ucgcgaaauc aagagcuccu cuaagaaacg ggcccagauu     2280

acgaauaaca auacaaagaa ccagaaacuu aauaaccuca ucaagagccu gcugaucgag     2340

auggcguccg auaccaaaua cgagaaugug acccccuaua gcccuucuca gcaagaaauu     2400

cucaagaucu acgacgaggg gauuuauucc aaagaagaga acagcgaaaa gaucaaggag     2460

auuaauaaaa ucagaaacaa uaacaagauc ucuaaauccg auauuacaaa guacaaacug     2520

uggcuugagc agggauauau cagccccuac accggcaaga uuaucccccu gacaaaacuc     2580

uucacggcca aguaucagaa ggaacauauu aucccucaga ccaaauuuuu cgacgauucu     2640

uuuaauaaca agguuaucug ugaggcugaa gugaacaaac ugaaggacaa uagccuggcc     2700

augaacuuca uuaaggcuaa aggcgggaag gccgucaaac uuuccgaugg agagacagug     2760

accauccuga aucaagaaga guacaagcag cucguccgca ccagcuuuaa aaagaacucu     2820

auuaagcugc ggaaucuccu gcuugaggaa aucccggaca ccuucacgaa aagacagcug     2880

aacgacacca aguacauuuc caaacucgug cugagccugc uuuccaauau cguucgggag     2940

aagggcgaac uggagucuac agccaaacgc auugugagca ccaacgggca aaucacaucc     3000

auccucagaa augauugggg acugaacaau auuuggaaca agcucaucac accccgguuu     3060

gaaagacuga auaacauuaa uaaaaacaag aucuauggca auugggagaa caagaaaggg     3120

aagaacgucu uccagauuaa uuaccuuaac gacaaucugu cuacgcucaa cacaaaacgc     3180

aucgaucacc ggcaucacgc ucuggacgcg cugaucgugg ccugcaccac aagaaaucau     3240

auuaacuucc uuaauaacca guaugcuaag agccgcaauc agagacacga ucugcgggcc     3300

aaacucaagc gcagagagac caucaccaca gaaggcucca agaaacaagu uuuuaaggac     3360

uucauuaaac cuugggaguc uuuuacgcag gaugcccuga agaaacucga aaccacaauc     3420

gugaacuuca aggucaauaa gcggauuauc aacaaaacca ucaauuggua caagaaaugg     3480

gagaagcaga aagacggaac auauaagaag auucagaaaa agcaaaccaa aggcgaugcu     3540

ugggccaucc gcaagccccu gcacauugac acgaucuacg ggauugugug uaaaaagcug     3600

ccucuuaaaa gcgcgcugga gaacuauaag aauaucauug augaaaaccu caagcauaaa     3660

aucaaggagc ugaaagaagu gaacaaguuu gacaauaacc agaucauuaa uuucuuuaaa     3720

gagugcaacu ucaucuuuga agacaauaac auucagaagg uugaggucua cgauuucaag     3780

gacaauagag auaaaacaau ccuuuccagc ucucggcagc ugauugacga guccuuuacc     3840

cccaagaaaa ucggaaaggu gcucgauccg accauccgca acauucugcu caaacaccug     3900

aauagcacaa aguacaacaa uaaguucgac ucugauggca acccuaucaa acccgaaaaa     3960

cuugcuuucu ccccugaggg gcugaaggac cugaauaaga acauuaaaca ucucaauaac     4020

aacaaggaac accaacccau caagagagug aaacuguuug agacccuugg aaauaagauu     4080

aaagucggcu auacacagca gaagaacaaa aaguucgccg aaacggccac cgggacaaau     4140

cuguuucucg gcaucuacag cgauaacaau aagcgggacu ucaaaaccau cccucugauu     4200

cucgcuaucc aacgcgagaa ggagggacug aaccccuguc cggauacaga cgaaaaaggc     4260

aaucagcuuc uguuuucucu guccccuaac gagcucgugu ucguucccaa uaacgaagag     4320

agaaagaauc ccaaccuggu ggauuuugaa aaucuuagca aagaccagau ugaccggauc     4380

uauaaguucg ucuccugcac cgggagcgag gccuacuuug ugccuucuag aacggcuaaa     4440

ccgauuaucg agaacgaaaa uggaaccaac aacaagagcc agcgcauuau cgaguucaau     4500

gaaaagacau ccaucaaaaa cgagaagaau gagcccguuc ugauuaaaac caucuguugg     4560

aagauugaag ugaaccggcu cggcaaaauc auuaagguca ucaaguga                  4608


<210>  4608
<211>  4608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4608
augaccaaga uccugggccu cgaccugggg acaaacagca uuggaugggc caucagagau       60

acggagaaug aaggcauuaa ccagauccuu gacaaagggg cucggauuuu cuccgaggga      120

gugaagucug aaaauggcaa agagaucagc cgcgccgcgg aaagaaccgc uuaccggucc      180

gcccgcaaga ucaaauauag acggaagcug cgcaaguacg agacacucaa aguccugucu      240

auuaacggga ugugcccccu uagcguugag gaaguggagc aauggaagaa auccggauuu      300

aaggaauauc cucugaaucc ggaguuccuc gauuggcuga gaaccaacga ggacaaaaau      360

aucaaccccu accuguuucg ggauaaggcc ucuaagcaga aagugacacu cuucgaacug      420

ggccgcgcuc uuuaucacau ugcccagaga cggggcuuuc ugagcaaucg ccucgaccag      480

uccgcugagg gggucuucga ggaacauaac ccucaaaucc agaaucugau ugaagaucuu      540

gacagcucua acaccauccu gaaugagcuc aaggaguacu auauuaaccu gggaaucauc      600

gaugaaacag agaaauccgg cuuuaagaaa gaccuggaug aaggggagaa gaaacucaag      660

agccuguaca auucucuugu ggccauuacc aagaaaaacg cgaacgacau cgaaacgugu      720

aagcaggagc ugauugccag acucaauaaa aaggaggauc ugggaaaagu uaagggcaag      780

aucaaagaca uuucccaggc uaugcuugac cggaacuuca agacccuggg gcaauacuuu      840

uucagccucu auaauaaaga acgcaucaga aaccaguaca caucucggga ggaacacuau      900

cuggaggaau uuaucauuau cugccagacc cagggaauug agggcaucaa uacaaacgag      960

aagcugcccg aaaagaaguu caccggccuc gccaaggauc uguacagagc uaucuuuuuc     1020

caacgcccuc uuaaauccca gaaggggcug auuggaaaau guagcuucga gaagaauaaa     1080

ucccggugcg ccgugucuca ucccgacuau gaagaguuua gaauguggag cuaccucaac     1140

acgaucaaga uuggcacaca guccgagaag acccugcgcu uccuuacacu ggaagagaaa     1200

cucaagcugg ucccuaaauu uuauagaaag agcgauuuca auuuugaagu gcuggcgaaa     1260

gagcucguug aaaagggggc cucuuucgga uacuacaaau ccagcaagaa gaacgaguuu     1320

uucuauuggu uuaauuacaa accgaccgac ucugugagcg cuugugucgu guccgccucu     1380

cuggagaacg cuaucggcaa ggauuggaaa auuaagaccu ucgaauauca aacacggaau     1440

acggagaaaa acgaagugac caagagcguc gacuacaagg aucuuuggca ucugcucucc     1500

guugccacaa gcgacaccua ucuguacgau uuugccaucg agaaacugaa acuugaaccc     1560

aaaaaugcua aggcguucuc uaaaacaaag cucaagaaag acuuugccuc ccugagccug     1620

gcugccauug ccaagauccu cccuuaucug aaacaggggc uucuguacuc ucacgcugug     1680

uucauggcca acauugagaa caucguggau gcggacaucu ggaaggacga ggaacagcag     1740

aaauucauuc aauccaagau cgucgagcuc guggauaauu acauuguuga aaagagcaaa     1800

cuggagcuga ucaacggacu ucucaagauu uauaauaccg aagacaaaga gggccgcaag     1860

guguacuauu ccaaagaggc ugaaucucug uuugaggccg aucugcggaa gaaacucguc     1920

cccuucuaca aggccaacau caucauugag gaacacgagc aggaaaucau uuuucaggac     1980

cuguucccua uccuuaugga ucagcugaag aaacaagagu uuauuaagau cggcagacuc     2040

gacaaacaga ucgaagcguu ccuggagggg gaaaaugagg agggacagau cuuuugcaac     2100

cacacggaua agcugaaaaa gcucuaucau cccagcgaca uugaaguguu caagaaaaag     2160

acaauuaaag augagugggg caaugaaaag guugugcuug gguccccgcu gaccaccucu     2220

aucaaaaacc cuauggccau gcgcgcucug caccagcucc ggaagguccu gaauacacuu     2280

auugccaacg accaaaucga ugaggacacc agaauccaua uugaaauggc gcgcgagcug     2340

aaugaugcca acaagcggaa aggaauccag gacuuucaga augagaacaa gaaauucaga     2400

gaagaggcua uuaaggaaau caaaaagcuc uaccuggagg aaugucacaa ggacgugaau     2460

cccacagagg augacauucu gcgguaucag cuuuggcucg agcaaggcaa augcgaaauc     2520

uacgaggaag ggaacaacau uagcaucugu gauaucauug gcuccaaucc uagcuacgac     2580

aucgagcaca cgauuccccg cucuaucucc caggauaaca gccagaugaa uaagacccug     2640

ugcucucuga aauuuaacag auccauuaag aaacaaaaga ugcccgucga gcucagcaau     2700

uauaacgaca uccugccucg gaucgcccau uggaagaaag aagcugagga acuuacaaga     2760

cagauugaga ccaucucucg cagcauuaag uccgccgcga caaaaguggc caaggauaaa     2820

aauauccgga agagacacua ccugacccuc aaacgcgacu auauucuggg aaaguacgaa     2880

agauucacgu gggaggagcc gaagguuggc uuuaaaaaca gccagauccc cgauaccggg     2940

aucauuacaa aguaugcuca ggccuaccug aaaucuuauu ucaagcgggu ggaauccguc     3000

aaaggaggcg cuguggccga guuccgcaaa cuuuggggga uccaagagag cuuuauugac     3060

gaaaauuggu ggaagcauua caaagauaag gacagagaua aacacaccca ucacacaauc     3120

gacgccauua ccaucgcuug uaugccuaag gacaaauacg aucugcucgc gcacgccugg     3180

cggcuggaag acgagcagga uaagaaggcu gccaaaguuc ucauugagca ggccaagccc     3240

uggaaaacgu ucaaggaaga caucgagaaa aucgaaacag agauucuggu gucccauuuu     3300

accccugaua acgucaagaa gcagucuaaa agcaucauua agaaucgcgg aaagaaggug     3360

uaugugcuua aaaacgaacu gcccguuaau uucaagaaca aaaucgaggg caaggacuac     3420

uuuaagcuca aauucgauuc caagauucug uauaaaaucc cgaagaaaaa ggagaagcaa     3480

acagacaccu uuuacgagga acugccuaaa aauuaucuua acggggucga aggcaaggau     3540

uacuucaaaa ucaacaccac aggaaagacg uuuuauaaaa uucccaucuu caaucagggc     3600

gacaccauua gagggucucu gcaccaggag acaaccuacg gagcuaucaa gcucccugau     3660

auugacaucg agacaaagaa accccugcau accgacaagg gcggguuuau ccucaaaaag     3720

gauauuaaag gaaacgaaau cguguucuuc gugguccgga aggagauuag caagaucucc     3780

gaaaaugacg ugcagaacau uguugauaau gugguccgca aaaagaucga gaacgccauu     3840

gcuaauucuc ugaucacguu uaaaaucgug aagaaaaaga aaguugcugu gauuaaggaa     3900

ggcgucacaa ucuggaugag agagccuaac auugagaagg ggaucgaagg cauucccauc     3960

aaaaaggugc ggaucauuac caauagcguu aaaaacccga ucgagauuaa ggugcacucc     4020

ccucuuagca aaucucgcca caagcauaag caaaaagucu acggacagaa ugacgaaaac     4080

uaugccaugg cccuguacga gcucgauggc aagagagagu ucgaacugau caauaacuuu     4140

aaucuggcua aacuucugaa gcagucccaa agcuauuacc cccuccacaa agagaaggaa     4200

auuaagggga aaaagauccu ggugcccauc gagaaacgga acaauaagga cgugauucuc     4260

aaaagaggac agcaggucgu uuucuaugau aagaccgugg aaaacccuaa ggacaucucu     4320

gagauuaucg auuuucgcga acggauuuac aucauugagg gccugacaau ccagagacaa     4380

aaagacaaga aaaccuccaa ggugaacgag uaugggauca uucagcuucg ccauuucaaa     4440

gaagccagaa agagcgagga aaucucuaag gauaauuuua aaccggacgg agaguucaag     4500

auuaacgaga auaaacccac acggaagaug aaccacaauc aguuuacggc guucgucgaa     4560

ggcaucgauu uuaaagugcu gccuuccggg aaguuccaga aaauuuga                  4608


<210>  4609
<211>  4614
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4609
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuacaacg       60

aaugauuucg acaccaagaa aggcacaauc gaggggcugg gauccagaau uauccccaug      120

ucucaggaua uucucggcaa guuugacagc gggguguccc ugucucaaac cgcucagcgg      180

acagaauacc gcggaaaaag acggcuuguc cagcgcuucc ugcucagacg ggagcgccug      240

cacagaguuc ugcauauccu cgaacugcuu ccuaagcagu auagcgaggc caucgauuuu      300

gacaagaaac ugggccaauu caagccggaa cgggagcaga aaauuuccuu uuacaagaac      360

gagucuggca aaaaucaguu caucuuuacc aagagccacc aggaaaugcu cacagaguuc      420

caaaccuauu accccacgau uaagaacauc ccuuaugauu ggaccauuua cuaucugcgc      480

aaaaaggcgc uuacagaaaa gaucuccaag gaggagcugg cuuggauucu ccugaauuuu      540

aaccagaaaa gaggguacua ccagcugcgg ggagaagaga aggaagagaa uaaaaacaag      600

aaggaagagu uccauacccu ccuggugaca gacaucaucg agcgcgauaa aggcaagagc      660

gggaccuggu auaaugugau ucuugaaaac ggauggaucu acaaaagaga gucuaagauu      720

ucccuggccg acuggaucgg caaaacaaag agcuuuauug ucacgaccga auauaaugau      780

gacgggucug ugaagcucac aaaagauaac gaggaaaagc gguccuuccg cagccccaaa      840

gaggacgacu ggggacugau caagaucaga accgagaaaa ccauuaauca gucugauaag      900

uccguuggcg ccuacaucua ugacgcccuu cugaagaacc cuaaucaaaa gauuaagggc      960

gaacucgugc ggacaaucga gagaaaauac uauaaggaug aacugcgcga gauucugaaa     1020

aagcagcucg aauuucacag cgacguccug aacgauaaga uccuuuacaa aaagugcauc     1080

gacgagcugu acaaaaacaa ugauucccau aagaacucua uuaaagaccg gggguucgau     1140

uaucucuucc uggacgauau cauuuucuac cagagacccc uuaagagcaa aacgucccug     1200

aucagcgacu guucucucga guuccgcccu auuaagaaua agaacggagu gcugguuaaa     1260

gacaccgaug gcaagcaaau cgcuaaagcu cugaagugca uugcccgguc caauccgauc     1320

uuucaggaau uuagacuccu gcaguuugug aaaaaccuua agaucuauaa gcgcgggguc     1380

guggacgaug uggaggucac aaaugacuac cugaaaagcg aaaacgaugu ugagaaacuc     1440

uucaauuggc ugaacgacaa agcggagauu aaucagaagc aauuucuggu gaacuucaaa     1500

cuuaaggaag auaagcaccg guggaauuuu guggaggaca aaggauaucc cuguaacgaa     1560

accagaaauc aguuccucaa cgccaucgcu aagauugaug ucgacaaauc uuucuucaac     1620

acaaagaaua cccaggaucu guggcacauc cuguacagcg ugacggacaa aauugagauc     1680

acaaaggcca ucaccaccuu ugcuaagcgc cauaaccucc cuaaugaauu uauuaucaac     1740

uucuccaaaa uuaagcccua uaaaaaggag uacggcucuu uuagcgagaa agccaucaag     1800

aaacugcuuc cucugaugcg guucgggucc uacugggaug cgaagaauau ugacaucaag     1860

acacucgaca gaaucgauaa aauucagacc ggagaauuug acgagaagau ccgcaaccgg     1920

guuagagaaa aagccauuga ucugcacaag aucgacgauu ucaaagaccu gccccuuugg     1980

cucgcuagcu auauugugua cgauaagcau ucugaggccu ccgacgucag caaguggaaa     2040

acaccggcug auaucgaaaa guuucugaaa cacgaguuca agcaacacuc ucugcggaau     2100

ccuaucgugg agcagguuau uacggaaacc cuccgcgugg ucaaagacau cuggcugcag     2160

uauggcgagg gcaaggaaaa cuuuuucaau gagauucaua ucgaacuugg gagagagaug     2220

aagaacgaua aagccacacg ggagaagauu uccaaaaagg ugagcgaaaa ugagaacacc     2280

aaucugagaa ucaaagccau ucucacagaa cugaagaacg acggagucuc cgauauccgc     2340

cccuacucuc cuagccagca agagauccug aagauuuaug aggaaggcgu guacaauaac     2400

gagucccgga aagaaaaacu ugaggaaguu gacaaaauca gaaagaacgc uaaacccacc     2460

acgucugaga uuaucaagua uaagcucugg cuggagcagg gguacgcgag ccccuacacc     2520

ggaaaacuga uuccuauguc cgaacucuuu acaaccaagu aucagaucga gcacaucauu     2580

ccgcagagcc gcuucuuuga cgauucucug uccaauaaag ugaucugcga aagcgagguc     2640

aacgaacuua agucuaauau gacagcccug gaguucauuc ucaccaacga gaauagaauc     2700

gugacgcuga caggcgggga aaccguuaaa auuuuuacaa aggaggcuua caaccaacau     2760

aucaucuccu auuacagcaa aaauaagaac aagcagaaac ggcugcuuac cgaagagauu     2820

cccgaacagu ucaucucucg ccaacucaau gacaccaagu auauuagcaa aaucgugaag     2880

aaccugcugu ccaaaauugu cagagaggag gaugaacagg aggugacaag caagcacgug     2940

guuucucuca auggcuccau cacgagccgg augaagcagg acuggggacu gaacgauguc     3000

uggaauacca uugugucccc ucgcuucgaa agacuuaaca cacugaccaa uucuaacgac     3060

uuuggccagu ucgagcucaa aaaggaugcc uacgggaacg agggaaaacg gguguuucaa     3120

acaaccgucc ccgacgccau cgcuaagggc uucacgaaaa agcgcaucga ucauagacac     3180

cacgcccugg acgcgcuggu gauugcuugu guuaaucgga cacauaucaa cuaucuuaau     3240

aaccugaaug ccaaggauac cgacgacaaa gccgugaagc acgaacuccg caacaaacug     3300

ugcuuuaaga ccaaaccuga uagcaauggg aacuacaagu gggaguucca uaagcccugg     3360

gaaaaauuua caaaggaggc ucaggacaaa cucaauacca caauugucuc cuucaagcag     3420

aacacgagag ugaucaauaa aaccguuaac aaguacgugu cuuauaaaga ugaaaaugga     3480

aaccugaaug ucgacgccaa cggccuuccg cagaagaagg ugauuacaca aaccaaaggg     3540

gauaauuggg cuauccggaa gccucugcac gccgagacag uuagcggcaa aguguuucuc     3600

aagagaauca aacaguccca cauuaccauc gcgaacgcca uugagcaggu cgaacugauc     3660

guggacaagg aggugaagaa acagcugaag gcuaaaauua aacuuuaccc caacaauauc     3720

gccggacuga agaagcaucu caaggcuuuc ccugucauga ucaacgauaa agccauugac     3780

aagauccaaa uuuaugaaac gcuggaggcg accgccacac gcaaaauccu cgauauuacc     3840

uuugacgaaa agaaaaucca gaagauuaca gauaccggca uccagaagau ccugcuuaau     3900

caccugaaac aagagaagua ccagaacaca guugacgaga augggaaaaa gcucgcuccc     3960

caugaaauug ccuucucuga gaacggacug gaagaucuga auaaaaacau caccuuccuu     4020

aaugacggca agaaccacca gccuauuaaa aaggugcgga ucuuugagga agggaauaag     4080

uucccccugg gagagguggg caacaaaauu aauaaguuug ucgagacggc uaaagggaca     4140

aaccucuucu uugccgugua ugaaaaugac gauaagaaaa gaaacuacaa gaccaucccg     4200

uucaacgagg uuaucgaacg ccagaagcaa ggccugaaug ccgcucucga gauugacgag     4260

aacggaaauc ggcugcuuuu uacccugagc ccuaacgauc ugguguauau cccccucgaa     4320

gacgagauug gcuaccugaa uggggucuac uucaaaaacc uuaauaagga acagacagug     4380

agaaucuaua aauuuguuuc cuguaccgga ggcgaggggc auuucgugcc caacaauuac     4440

agcaaggaaa uuaucucuaa cgagaaugga uccaacaaua aaaacgagcg caugcugaau     4500

uuuaacaacu ucaauaucau uuuugauggc aaggaaaagc cugucaugau caaaagcauu     4560

ugcuggaagc ucgaggugga ccggcugggg aaaguuauca agauuaucaa auga           4614


<210>  4610
<211>  4617
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4610
augaagcaca uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaaa       60

uccgagcagg auugcgacga ugucgaaaga aucaauggca uugaggcugc cgggucucgg      120

aucauuccca uggacgcggc uguucuggga gauuucgcca agggcaacac aaaaagccaa      180

acggccgacc gcaccgcugc cagagggauc cggcgcaugg gagaaagaca uaagcuccgg      240

cgcgagagac ugaaucgggu gcuugaugug augggcuuuc ugccugaaca cuacuccgcu      300

gcccucacac gcuauggcaa auucaaggac ggggaggagu guaagaucgc guggagaaaa      360

gaugaaucug gacaguggac cuuugucuuc gaggacagcu uucgggaaau gcuggccgau      420

uucguggcuc aucagccgga guggcuggcc ggcgggcgca agguucccua cgacuggaca      480

auuuauuacc uccggaaaaa ggcucugucc aaaccucuua ccaagcagga gcuggccugg      540

cuccugcuua acuuuaauca aaagcgggga uauuaccagc ugcgcggcga agaugagcac      600

acagacgaca aagggaagcu cguggaauau uacgcgcuga aagucguggg aguugaggau      660

accggcgaca agaaaggcaa ggauacgugg uucaacauca ugcuggaaaa ugggauggug      720

uacagacggc ccgccgcuga ggccccugac uggauuggaa aaaccaagga guuuaucguc      780

acaaccgauc ucgaaaagga cggcacaccc aaaaaggaua aagaggggaa cauuaagaga      840

ucuuuccgca ugccuaaaga agacgauugg acccugauca agaagaaaac ggagaaugac      900

auugaucgga gcggaaagac agugggcgaa uauaucuacg acgcgcuucu ggccgacccg      960

acccagaaaa ucagagggaa gcucgugcgc acagucgagc ggaaauauua caaggaggaa     1020

cugaagaaaa uuaucaacuc ccagaagaga uuuauucccg agcuuaauga uaacgaacug     1080

uauggacgcu gcaucagcga gcucuaccaa ucuaaugacg ccuaucggca guccauuagc     1140

ucuugugauu ucaccuaccu guuuguugac aacaucaucu ucuaccagag accucugaaa     1200

uccaagaaaa gccucauuga uaauugcccc uaugaagaac auucuuacac cgacuccaag     1260

acaggcgagg ugaagacggc uccugugaaa ugugucgcua agagcaaccc ccuguuccag     1320

gaauuucgcc uuuggcaauu cgugcagaac auccggauuu uugagagaca gaaaaagauc     1380

ggcgauuccg uuaaaaccga cguggauguc acaggggagu ucauuaaguc ugccgacgau     1440

gugguuagcc uguuugacuu ccucaaugau cgcaaagaca ucacccaauc cgugcugcuu     1500

ggaacauauu uuaagaucaa gaaaccgaag ggcaaagaca gcgauuaccc uuaucggugg     1560

aacuacgucg aggacaagac cuaucccugc aaugagacga gagcucugau ucucaaaggg     1620

cugaaggaug ccggaaucga cgaaggcuuc cuggauaagg acaaagagua cucucucugg     1680

cacauucugu acuccgugga agauaagcag gagaucacaa aagccaugga aaaguuugcu     1740

gccaaaaaca gccuuggggg caaguucgcg gaggucuuuu cuagccugcc ucccuucaau     1800

aagaacuaug gauccuacuc ugagaaagcu auuaagaaac uccuggcccu gaugcggacc     1860

ggcaaguauu ggagcgccga cgcuaucgau aaacacaccc uugaacgcau ugacaagauc     1920

aucaaugggg aguacgauga auccauuaga acacggguga gagagaaggc caucaaccuc     1980

accgacguua gccauuuucg cggacugccc cuguggcucg cuuguuauau uguguacgau     2040

cggcacucug aauccaaaga caucacaugc uggaauuguc cugccgacau ugaucaguau     2100

cuggaccauu ucaagcagca cagccuuaga aacccgaucg ucgagcaagu gguuacggag     2160

acccugcgca cagugagaga uaucuggcgg caguacggca aaauugacga aauccacauu     2220

gagauggggc gcgauaugaa gaaucccgcg gacgucagag ccaaaauguc uaagcggaug     2280

aucgauaacg aaaaugcuaa ccuccgcauu aaagccaugc uggcugaguu cgccgacccu     2340

gauuuuggaa ucgcgggcgu gagacccuac uccccuagcc agcaggaaau gcugcggauc     2400

uaugaggagg gggcccuuga auccguggac gagguucccg augaaauuaa ugagaucauu     2460

ucuaaguuca accaaaccga caucaagaaa cgcccgacac auggcgagau uaucaaguac     2520

aaacucuggc uggaacagaa guaucagagc ccuuacaccg gaagaacgau cucccugucu     2580

cggcucuuua ccgacgauua ucagauugag cacgucaucc cccaaagccg cuacuucgac     2640

gauuccauga gcaacaaagu gauuugcgaa gcugagguga aucagcugaa ggacagaaug     2700

cuuggcuaug aauuuaucaa ggcccaucac ggggagaaag uccugcucuc uuccggagag     2760

acagugacca uucugacagc ugaagaguac gaacggcugg uucagcuuaa cuucagaaag     2820

aauccuguga aaaugaagaa acuccugaug gaugacaucc ccgauaaguu uauugagcgc     2880

cagcugaacg acagccggua caucucuaag aaaaucaagu cccuccugag caauaugguc     2940

agagaugaag gcgagcaaga ggacauuucu aaaaacguga ucgccuguaa uggggccauu     3000

accgaucgcc uuaagcggga cuggggaguu aacgaugugu ggaaucauau cauucugccu     3060

agauucgaac gccucaacgc ugucacgggc agcgaccggu uuacaaccac auccaccaau     3120

gggcacacca uccccgacau gccgcugaac cugcagaaag gcuucaauaa gaagagaauc     3180

gaucaccgcc aucacgcgau ggacgccauu gugaucgcuu gcacaacgcg gagccauguu     3240

aaccuucuca auaacgaggc cgccaugucu aaaaacaaug cugugagaua ucagcugucc     3300

cggaagcugc gcagauacga agauaccaug gucacacgga acggagagac ccgcacaauu     3360

agcguggcca gagaauuucu caaaccuugg cccaccuuca augcggacgu ggagaaggcu     3420

cugcggggca ucgucguuuc cuuuaaacaa aaccuuagag ugauuaauaa gacggugaac     3480

aaguaucaga aauucgucua caaugaggaa aagcacauca aagagaagaa agccgugcgc     3540

caggaagaga gaaacgggag acaucugcuc gccauccgca agcccaugca caaagauaca     3600

guuuauggag aggugaaucu gcggagaauu aaggaagucu cucugaagga cgcuguggcc     3660

aacccuaaag cgguugugga uaaggaccuu aaaaagaaac ugauggcuau gaucgagcuc     3720

ggcuacgauc ugaagcagau uaccaaguau uucgaagaca auaaagaugc cuggcaagac     3780

gucaaccuca agaaaaucaa gguguacuac uuuaccaaag auacagggga ccgcuauuuc     3840

gccacccggu uuggaaauga ccugguuaca cuuuucagca agauuacgaa ggcugaugac     3900

gccaaaaagc agaucgagag aauuaccgau acaggcaucc agaaaauccu gaagcgccac     3960

cucgaagcua acgggggcga cugccagaaa gccuuuuccg cggauggaau ugacgagaug     4020

aauagaaaca ucacccaacu gaacggcggg cggccgcaua agcccgugua caagguccgc     4080

auuuaugaga aagccgauaa guucgcuauc ggaacaaaag gcaauaaggc cgcuaaauuu     4140

guggaagccg ccaaggggac caaccuguuc uuugcugugu accuuaaaga guaugaagac     4200

ucuacgaccg gagagacaau uaagaagaga gucuucgaua ccaucccucu gaaaacaguu     4260

aucgaccggc ucaagaaugg ccagaccccc gcgccugaag agaaagaugg cgccaagcug     4320

cucuuugugc ugagccccaa cgaccuugug uaccugccua cagaggacgu cgugaaaaau     4380

gggaccauua accgcccccu cgauaagaga cggaucuaua agaugguuuc cuguacggga     4440

acagaaugcu acuacauucc guucaucgug gcuucuccua uugucgacaa aguggaguuc     4500

uuuagccuga auaagaucgg cagagccauc accggggaaa ugauuaaaga gaucuguauu     4560

cccaucaagg uugaucgccu gggaaacauu accgaaguga auaacgagaa aaucuga        4617


<210>  4611
<211>  4623
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4611
augaacaaga uccugggccu cgaccugggg accaauagca uuggaugggc ccuugugggc       60

acagaugagg aagggaaccc caucagaauu uacaaauccg gaucucggau cauuccuaug      120

agccaggacg uccugggcaa uuucgaucgc gggaacucca ucucucaaac ggcugagaga      180

accagcuauc ggggaguucg cagacuccgg gaacgccauc ugcuuagacg ggagcgccug      240

cacagagugc uccaccugau gggcuuucug ccgucccauu acgaagaggc caucggcugg      300

gacaaggggg auaauaaaac auucgcgaag uuuuauucug acaccgagcc caaacucccu      360

uggcggaaga acgcugaacg cgagcacgag uuccuguuua auacacccua caacgagaug      420

cuuguggauu ucagaaagaa ucagccucag cuguuuaaaa uuaagaaaaa cggacaggag      480

agccucaucc ccuaugacug gaccauuuac uaucugcgga agaaagcccu uuccaagccu      540

aucagcaaga aagaacuggc cuggauuauc cucaauuuca accaaaagcg cggcuacuac      600

cagcugagag gggaggaaga ggaagaaaau ucuaacaaac ugauugagua ucaugcucuc      660

acagucguga agguuaucgc cgaugaaccg cagaaaggaa aguccgacau cugguacaau      720

gcugagcugg aaaacggcug gguguaucgg agaagcucua aaacaccccu uuuugauugg      780

gaguccaagg ucaaggaguu cgugguuacg accgaccuga acgccgaugg gagccucaaa      840

guggacaagg agggaggcgu caaacgcucu uuucgggugc cuuccgagga cgauuggaug      900

cuggugaaga aaaagacaga aagcgacauu gagaaguccg auaaaaccgu cggcugcuac      960

aucuaugaca caauucuuca gaaucccucu caaaagauca gagggaaacu gguucgcacc     1020

auugaacgga aguucuacaa aaaggagcuc acggaaaucc ugaacaagca gaaagaguuu     1080

cacagcgagc ugcagaauaa ggaacucuau caggagugua ucaaagaacu guacccucac     1140

aacgaggcgc auuacgauuc ccuucuggug aaggacuaua cagaucucuu cgugaaugac     1200

auuaucuucu accaaagacc ccugaaaagc aagaagucuc uuauuuccaa cugcccguau     1260

gaguaccgcg ucuaucggaa ugauagcgga aucguggaaa gaguuccuau uaaauguauc     1320

gccaagucuc acccccugua ccaggaguuu cgccucuggc aguucaucca uaaccugcgg     1380

auuuaugaaa gacaacgcga ggugaauggc aaaccugaaa ccgacgucga uaucacagcu     1440

gaguuuauuc ccaccaccga ggaccgggug aagcuguucg acuggcucaa caauaaaaag     1500

gaaaucaaac aggauacacu gcuucagagc uacuuuaaga uuaagaaaaa gaaaggggac     1560

gauaaguucc ccuacagaug gaacuauguu gaggacaaag aauacccuug caaugagacg     1620

cgggcccuga uccucucccg ccugaccaag cugaaccuuu cugaagauuu ucucacaaag     1680

gagauggagc uggcucugug gcacauucuc uauagcgugg aagacaaaga ggaaaucaag     1740

aaagccuuca aguccuuugc gcagaaacac aaucuggaug acagcuucgu cgaucaauuu     1800

aucaaguucc cgcccuuuaa gaaagacuac ggaucuuauu ccgccaaggc uauuaaaaag     1860

cugcuuccuc ucaugagaau cggcaaauac ugggaugaga aggccauuag cgggaacacc     1920

cggucuagaa uccuguccau uaacgaacgc cuggaggaca ugcggagcuc cggaaagaaa     1980

aucgaggaug ugucugacga cgauaucccc aagcaggucc uuaagagcuu cgacaaagug     2040

aauggcguuu auuccggccu caacacauac caggcuuguu acgccgugua ugggagacau     2100

ucugaagcca gcgagauuac caaauggaag acgccucagg auaucgacga uuaccugaau     2160

gaguucaaac aacacucccu gcgcaacccc auugucgaac aggugaucac agaaacccuc     2220

agaguugugc gggacauuug gaagaaguau ggagauuuua gcgaaaucca uaucgagcug     2280

ggccgcgaaa ugaaaaaucc ggcugacaag agagcgaaaa ugaccacaca gauuucugag     2340

aacgaaaaua ccaaccuucg gaugaagucc cugcucgccg agcugaaaaa ucacaacgau     2400

gucgagaaug ugcgcccuua cagccccucu cagcaagaaa uccugaagau uuaugaggaa     2460

ggggugcuua acuccguuag cgagaucccu gaagagauua ucaagaucac aaaaucuagc     2520

cagcccuccc agacggagcu cgucagauac aagcuguggc uggaacagaa auaucggagc     2580

ccuuacaccg gagagauuau cccccucggc aagcuguuca caucugcuua cgaaauugag     2640

caugugaucc cgcaaucccg cuauuuugac gauagccuuu ccaauaaagu gauuugcgaa     2700

ucugagguca acaagcugaa ggggaaucuc cugggcuacg aguucgugaa aaaccaccac     2760

ggaguuaagg uggaacugaa cuuuaauaaa accgucgaga ucuucagcau uaacgaauau     2820

gaggaguuug ugaagggcaa uuacgggaaa aacagaggaa agaugaagaa acuucuccug     2880

gaagacaucc cuaaguccuu caucgagcgg cagcugaaug auacacgcua uauuucuaaa     2940

guugugaagg gccuccugag caacaucguc agagaagacg gggagcagga agccaccucc     3000

aaacauguga uucccuguac gggaggcguu accacaaucc uuaagcaaga cugggggcug     3060

aaugaugugu ggaacaagau ugucacaccc cgguuugaga gaaugaauga gcucaccaac     3120

accucucugu ucggccagug ggaaaauaaa gagggaaagc gcaucuuucg gacggaagug     3180

ccucuggagc uucagaaagg cuucaacaag aaaagaaucg accaccgcca ucacgccaug     3240

gaugcuauug ugaucgccug cgcgacacgg agccacguca ccuaccugaa uaacgaaucc     3300

agcaaggcua augccaaaga gauuagagac gggcucaagc auaagcugug uuuuaaaucu     3360

aagacagaug agaccggaaa cuauaaaugg caguuccaca agccguggga caccuucaca     3420

caagauguuc aggacacgcu cgaaaacauc gugauuuccu uuaaacagaa ucugcgcgug     3480

aucaacaaga ccacaaaucg guaccagaag uacgaggaag agaaaaagau ccuuaaaagc     3540

caagaaaagg gcgauucuug ggccauuaga aaaccccugc auaaggacac cgucuucgcu     3600

aagguguccc uccagaaaau caagugcguu aaacuggcca uggcucugaa ggauuggaaa     3660

aacauugugg acaagcagcu uaagaaagag auccgccggc ugauucucag cuaugggaag     3720

uuugacgccg agauccugaa uaaauacuuc aaggauagaa aauauauuuu uaaggacguc     3780

gauaucucua aaguggacgu uuacuauuuc gaaacagagu acguggcgac ccggaagaac     3840

cucgauacgu ccuuugaccg caagagcauc gaauccauua cagauaccgg aauccagaaa     3900

auucugcuua aucaccuggg cgucaagaac aauaacccug agcucgccuu cagcgcugag     3960

gggaucgacg aaaugaauca aagaauugug gagcugaacg gcggaaaaca gcaucagccc     4020

aucuauaagg uucggaugua cgaaccucug ggcaauaaau uucaaguggg gcgcaucgga     4080

aacaagaagg auaaauacgu cgaggccgcu cccggcacca aucuuuucua ugccauuuac     4140

cagucugaag acaagaaaag aaucuaugau acaauuccuc ugaacaucau uaucgagcgg     4200

cagaagaaag agcucuuucc cgugccggaa aauaaggagg acaaggauaa ccuguacaaa     4260

cuccuguucu ggcuuagccc uaacgacaug guguaucugc ccaccgaaaa ggagugggcg     4320

aauccugacc uguucaacuu uaauaacaug accaaaaauc agagaaagaa acucuacaag     4380

uucaucgaug gguccggaac gaccgccaac uuuauuccca auacagucgc uaacaucauu     4440

uucucuaucc cgaagaaaga cagcggcaag uauuccaugg ggaaucgcau uguucaaaac     4500

gaauuuggca ucggaucucc ucagagcaaa aaucagaagg ccuucaccgg cgagaugauu     4560

aaagagcggu guuggaagcu gaaaaucaac agacuugggg auaucauuga augcaucaag     4620

uga                                                                   4623


<210>  4612
<211>  4629
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4612
auggagaaga gaauccuggg ccucgaccug gggaccaaca gcauuggaug ggcccuugug       60

aaaggcgaac ggcacgagaa ugauaacgaa acaauccugu ccuacauuga ggcugccggg      120

ucucgcaucc ucccccugga cgcggcugcc cagggagauu ucgaaaaggg caauccugcc      180

aaaacgcaaa ccagcgagag aacacggaug cgcgggauga gacaucuucu ggagcggucc      240

cuccugcgca gagaacggcu gcaccgcguc cucgacauua uggauuuucu gccgccccau      300

uauucucaga gccuuaccag auacggaaag uucaaagcug acacagagug caagcuggcc      360

uggcggaaga ccgaaacagg ccacuaugag uuucucuucc aggauuccuu ucaggagaug      420

cugcaagacu uccagaacca uggcaaacuu gaagagaaua agaaaauccc uuacgauugg      480

accaucuauu accugcgcaa gaaagcucuc acgcagcccg uuucuaagca ggaacuggcc      540

uggauucugc ucaacuuuaa ucaaaagaga ggguauuacc agcgggagga ccaggcggug      600

gaugacaacc agaaaaagcg cguggaauac caagcccuga aagucaucag cguggaggau      660

accggagaga agaaaggcaa gaauacaugg uaugacguua gacuugaaaa cgggcuggug      720

uaccacuauu ccgcuccuau uaaacccgac uggaagggag agaccaagga guucaucguc      780

acaaccacga uugaucagaa uggcaaaccu gaacucgaca aggaugggaa accgaaguac      840

agcaugcggg cccccaaaga agacgauuau aagcugcuua uggagaagac aaagaaggac      900

aucaccgauu cuggaaaauc cgugggcgag uacauuuaug acgcucugcu cguuaacccu      960

cagcaaaaga ucagaggcgg gcuggugcgc acaaucgaac gguacuuuua caaaaaggag     1020

cuggaaaaga uucuccugag ccaggagaga uucauccccg aacuuaccga ucgcgagcug     1080

uaucggagau gugucgagga acucuacccu ucuaaugacg ccuaucgcca ccugauugag     1140

ucccgggaua ugagauaccu ucugguggaa gacauccucu uuuaucagcg cccccugaaa     1200

agcaagaaau cucugauuga cgauugcccg uacgaguccc ggagcuaugu ggacagagag     1260

accggagaaa ucaaguccuc uccugucaaa uguaucgcgc agagccaucc ccucuuccaa     1320

gaguuucgcc uguggcaguu cauucacaac cuucggaucu accagaauga aagauccguu     1380

gagggcaagc ugcagcucga uguggacgug acagaagagg uccugccuac gcccgagagc     1440

aaggccgcuc uguuccgguu ucuuaacgau cgcgaaaacg ugacccaaga gauucuccug     1500

aaagaaguuu ucaaggggag aaaacugucu gugaaggacu gccccuaccg guggaauuau     1560

gccggagagg gcaaaaagua cccuuguaac gaaacaagac aucucaugcu gaaacuucug     1620

aagaaggcug ggaucgauac cgccuuucuc acaccggaga aagagcaggc ccuguggcac     1680

auuauguauu ccgucgaaga caaggaucag uacgagaaag cucugagcac cuucgcgcgc     1740

aagaaugacc uucccgaugg auuuaaagaa caguucaaga aggccccuac guuuaaaaag     1800

gacuauggcg cuuacucucu ccgggccauc aaaaagcugc ugccccucau gagagugggc     1860

gaguauugga gcgccgauug caucgacucc gaaacaaaag agcgcauuuc ucaaaucauu     1920

agaggggaga uggacgaaaa gguuagccag cggaugacca agucccgcga uagcuucacc     1980

gacaucucuc aguuucaggg acugccugug uggcaggcuu guuacgucgu guacggcaga     2040

cacuccgagg ccacagaaau ucaacggugg gagaaacccg aggauaucga cgucuaucuu     2100

aaggcguuca aacagcauag ccugcgcaac ccgauugugg aacaaguugu gcucgagacc     2160

cugagagucg ugcgggauau cuggaagaca uacgggaaag uugacgaaau ccacauugag     2220

augggacgcg auaugaagaa uacggcugaa gagagaaagc ggaucuuuaa ccgcaaucag     2280

cagaacgaga augacaaccu gagaauuaaa gcccuucuca ccgaguucau gaauccugau     2340

uuugaaaucg aaggcgugcg gcccuauucu ccuucccagc aagaggcccu gagaauuuac     2400

gaagacgggg cuuggagcgg ccuggaugag gagaaggaag acaaaaagga gcucgaagcc     2460

auccgcgaga uccugggaaa auucucccag ucugauaagg cuaaacggcc cacacauagc     2520

gaaguccuua gauauaaggc cuggcuggag cagcgcuacu ccucuccuua uaccggcgag     2580

gcgauucccc ucgccaagcu guucacaccg gcuuacgaaa ucgagcacgu gauuccucag     2640

agccgguuuu ucgacgacuc cuuuagcaac aaagugguuu gcgaagccga ggucaauaag     2700

cugaaagaua gaaugcuugg guacgaguuc aucaaggcuc aucaaggaga aguggugucu     2760

cucuccggca ccgggcgcug ugcccggauu cugagccccg aggaauauga gcagagaguc     2820

acggacugcu acaaacacaa caccgcgaag augaagaaac ugcucaugga agagaucccc     2880

gaugacuuua uccagcgcca gaugaaugau ucucgguaua uuuccaagcu ggugaaaagc     2940

cuucugucua acaugguuag agaggaagga gagcgggaag ccaucagcaa gcacgugauu     3000

acauguaacg gcaccaucac agaccgccuc aaaaaggauu ggggcgucaa ugacgugugg     3060

aaccauauua uccugccuag auuccaacgg augaaggauc ugaccgggcg caaugacuuu     3120

guugcuguga acucccaggg acacgagauc ccgagcgugc ccccugaacu ucagaaaggc     3180

uucaagaaaa agagaauuga ucaucggcac cacgccaugg acgcuaucgu cauugccugc     3240

acgacaagaa aucauguuaa ccugcucaau aacgaggccg cuaugucuga gaauaaagcg     3300

aaccgcaucg cccugucccg gaagcucaga cgcuacgaaa auugcgcuau uuauaaaggg     3360

ggagagacca aaaacaucag cguggccaag gaauuucuga aacccugggc cacauucccu     3420

gcugacgucg agcgggcccu uggcgauauu gugguguccu uuaagcaaaa ucugagaguc     3480

aucaacaaag cgaccaauca cuacgaacau uauaaggacg ggaagaaagu uaaggugccc     3540

cagaaaaagg gagauaugug ggugguccgc aaaccucucc acaaggacac cguguacgcu     3600

cugguuaacc ugcggaacgu gaaaacaguc ggccuuaagg augccauccu gaugcccgac     3660

agaauugcca auaaggaucu caaaaagaaa gugaaggagc ugcucgcuca gggggacgau     3720

cugaaaaaga ucaagaaaua cuucgagacg gaaaaggaca ccuggcagga uguugacgug     3780

aaaaagauug aggucuauua cuuuucugaa gagacagcca accauuauua cgcgacccgc     3840

aaaccguucg augcuaaugu gaagaucgac agcguuacag acaccggcau ucaaaagauc     3900

cuuagacggc accuggagga aaacggaaau aaagccgaug uggccuucuc cccugagggc     3960

aucgcucgca ugaacgaaaa uauuuuuaga cucaacggga auaagcacca ucagcccauc     4020

uauaaagucc ggguguacga gaaggccaac aaauauccug ugggacagac gggcaauaag     4080

gcuucuaagu ucgucgaagc cgcgaaaggg acaaaccugu accuggccgu uuacgagagc     4140

gagguggugg acaagaaaac cgcugaaauu uccaagaaac gcucuuaugu caucgugccc     4200

cuuagagaug ccguugcucg gcagaagcaa ggagugccgg ucgccccuga gagaaaugaa     4260

gagggcggga aacugcucuu ugugcugagc cccaaggacu ucguuuacgu gccuaccgaa     4320

gaggagcgcu ccaagggcga aguccuccug ccccuugaua aaaaccggau uuauaucaug     4380

auggacgcca gcgauggaau cgugaauuuu cugcccuacu cugucgcuaa ccugauuuau     4440

gcgcucccua aggaccaggc cuccggcuuc uguacagaug ggagaaugau ccagaacgag     4500

uuuggacugg gcagcccgaa aucuaagaau gaacgcgcuc uuaccgggga gaugauuaaa     4560

gaaaucugcc ugcccgugaa gguugaccgg cucggaagaa uuacagagau caacggcaau     4620

aagguguga                                                             4629


<210>  4613
<211>  4644
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4613
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuuacaaau       60

aacgauuucg acaauaagcu gggcuccauc gaggggcucg gaucuagaau uauccccaug      120

agccaggaug ugcugggcaa auuugacucc ggggucucua uuagccaaac ggcugaacgg      180

accaaguacc gcggaacaag acggcuuguu cagcgcuucc ugcucagacg ggagcgccug      240

cacagaaucc ugaacauccu cgauuuucug ccucggcauu auuccgaagc cauugacuuc      300

gauaaaaagu uuggccaguu caagccggag cgcgaagaga aacuuucuuu uuacaagaau      360

gagagcggca aaaaccaguu ccuguuucuc gaaucccacc aagagauguu cagcgaauuu      420

aagcugaccc aucccgaccu uaaacaggug ccuuaugauu ggacaaucua cuaucugaga      480

aagaaggcgc ucacccagaa aauuucuaag gaggagcugg cuuggaucau ucugaauuuc      540

aaccagaaac ggggguacua ucaacuccgc ggagaagagg aagaggugaa uaagaacaaa      600

cuggaagagu uucacacacu ugucaucgug gacguuauug agagagaaaa gggcaaaucc      660

gggguguggu acaaugucau ccuggagaac ggauggaucu acaagcggga aagcaagauu      720

gcccucuucg auuggaucgg caaacagaag aauuucauug ugaccacgga gcugaacgac      780

gaugggacca ucaaacuuaa uaaggacaac gaagagaaac gcucuuuuag aucccccaag      840

gaggacgauu ggggacugcg gaagauuaga acagaacaga ccaucaacca gagcgagaaa      900

ucuguuggcu ccuucaucua ugacgaacuc cugaagaaac cuaaucaaaa gauuaaaggc      960

gagcuggugc gcacaaucga acggaaguuu uacaaggaug agcucagcaa aauucugaag     1020

gcccagauca ccuuccauua ugagauucuu ggggacagaa acaucuacaa aaagugcauc     1080

gaugaacugu aucgccacaa ugacucccac aaaaacaaua uuaaggauaa gggauuugac     1140

uaccucuucc uggaugacau cauuuucuau cagcggcccc uuaaaucuaa gacgagccug     1200

aucuccgauu guagccucga guucgucccu auuaaagaca agaaaggcau ccuggugaag     1260

gacucuaacg ggaaacaagu ggucaagccg cugaagugca uugcuaaauc caaucccauc     1320

uuucaggaau uuagacuccu gcaguuuguu aagaaccuua aaaucuacaa gaaagagauu     1380

aucaaugaga uugaugugac aaacgacuac cugaagaccg aagaggaucu cguggaacug     1440

uucgacuggc ugaaugauaa ggccgagauc agccagaaac aauuucuugc uuauccuaag     1500

uucaaacuca aggaagacaa auaccgcugg aacuucgucg aggauaagau uuaucccugu     1560

aaugagacac ggaaccaguu ucugaaugcc aucgcgaaga ucgacgugaa caaaucuuuc     1620

uuugaugcca agaacaccca ggaccugugg cauauucucu acagcguuac cgauaaaaca     1680

gaacuguucu ccaucaauac ggagaccggc auucagucug gagcucuuag cacauuugcc     1740

aagagacaca accuguccaa ugaguucgug agcaacuuuu cuaaaaucaa gccuuauaau     1800

aaggacuacg gguccuauag cgaaaaagcu auuaagaaac uccugccccu gaugcgcuuc     1860

ggaucuaacu ggaauaagaa caauaucgau gccaaaaccc uugcgcggau cgacaagcuc     1920

caaacaggcg aauuugacga gaccaugaga acgcgcgucc gggagaaagc cauucaucug     1980

uccaagauca cagauuucca gggccugccg cucuggcugg cuagcuacau uaucuacgac     2040

agacacuccg aagccucuaa cauuagcaag uggaaaaccc cugaggauau cgcuugguuu     2100

cuuaauccca agaacaaauc cggguucaag cagcacucuc ugcggaaucc uaucguggaa     2160

cagguuauua ccgagacacu ccgcgugguc aaagacaucu ggcaacauua uggagaaggc     2220

aaggagaacu uuuucaauga gauucacauc gaacugggga gagagaugaa gaacaacgcc     2280

aaugaucgga aaagaauuac cgaaacaauc aaccagaaug agaacacgaa ucugcgcauu     2340

aaggccaucc uuaccgagcu caaaaacgac ggaaucaaug augugcggcc cuacagcccc     2400

ucccagcagg aaauucugaa gaucuaugag gaaggcgucu acaacagcga gauuagaaaa     2460

gaaaagcugg aggacaucga uaagauucgc aaaaaugcua agccuacacc gucugagauc     2520

aucaaauaua agcucuggcu ggaacaaggg uacauuuccc ccuauaccgg caaacuuauc     2580

ccucugagcg accucuucac aaccaaguac cagauugagc auaucauucc ccagucuaga     2640

cuguuugaug acucccugag caacaaggug aucugcgaau cugagguuaa ugaucuuaaa     2700

ggaaacaaua cggcgcucga guucauccug aagaacgaag gccggauugu gcugcucacc     2760

gggggaaaaa cagucaagau cuuuaccaaa gaggccuaca aucaacacau uacaagcuau     2820

uacaucaaga acaaguccaa acagaagcgc cugcuuaccg aagagauucc ugaaaaauuc     2880

aucgacagac agcugaacga cacgaaguau auuagcaaaa ucgugaagaa ucuccugucu     2940

aaaaucguuc gggaggaaga ugagcaggag gugacaucca agcauauugu cagccugaac     3000

ggcuccauca ccucucgcau gaagcaagac ugggggcuua augaugugug gaacaaaauu     3060

gugagcccca gauuugaacg gcugaaugcu aucacaaacu ccaaugacuu cggaaagcuc     3120

gagcugaaaa aggaugaauu uggcaacgcc gggaaacagg uuuuccagac caccgucccg     3180

gacgauauug ccaagggcuu uucuaagaaa cgcaucgacc acagacacca ugcucucgau     3240

gcccugguga ucgcguguau uaaucggaca cacaucaacu accuuaauaa ccugaaugcu     3300

aaggacgaga acgauaaaac gauuaagcau gagcuccgca auaaacugug cuucaagacc     3360

aagccugaca gcaacggaaa uuccaaaugg gaauuucaca agcccuggga gaacuucaca     3420

aagaaggugg aagacgagcu gaaccagacc aucauuucuu ucaaacaaaa uacaagaguc     3480

aucaacaaga ccgugaauaa guaucagguu uggaaagaag aggauggcgc caugaagaaa     3540

gugacgguca agcagacaaa aggggacaac ugggccaucc ggaagccuau ucacgcugag     3600

accgugagcg gaaaaguuuu ucuuaagaga aucaagcagu cccauauuac caucgccaau     3660

gcuauugaac aaaucgagcu gauuguggau aaagaagucc gcaagcagcu caaagccaag     3720

aucaaacagu accccaacaa uaaggagggc uucaagaaac accugaaggc guuuccuauc     3780

augauuaacg ggaaagccau cgacaaggug caaauuuaug aaacaaucga ggcuaccgcc     3840

acacgggaga gaaauaaccu cgauauuacg uucgacgaga aaaagaucga aaagaucacc     3900

gauacaggaa uccagaaaau ucugcuuaau caucugaagc aggaaauuua ccagaacgcu     3960

aucgacgaga gcggcaaaaa gauugcgccc cacgaaguug ccuuuucuga gaucgggauc     4020

gaugagcuca auaaaaacau uaaggugcug aauaacggca agaaccauca accgaucaaa     4080

aagguccgca uuuucgaaga guccaaacgg uuuagccugg gacagucugg cuccaagacc     4140

gacaaauacg uggaaacagc caaggggacc aaucuuuucu augcuaucua cgagggcaaa     4200

aagggaaaca gaaauuauaa gacgauuccu cugaacgagg ugaucgaacg ccagaaacag     4260

gggcucaaua gcgcccuggu caccgaugag aacgaaaauc ggcuccuguu uacacuuucu     4320

cccaacgacc ugguuuacau ucccaccaau gaggaaaaga aaaaccugac aaccaucaau     4380

uucgauaagc ucuccagaga gcaaacagag cgcaucuaua aauuugugag cuguaccucc     4440

ggagaaggcc acuucgugag caacaauuac ucuaaggaga uuaucagcaa cgaaaauggg     4500

uccaacaaca agaaugagcg gaugcuggaa uuuaacaauu cuaacacgau uuaugacgag     4560

aaagagaagc cugucaugau caaaagcgug ugcuggaaac uugaaguuga cagacugggc     4620

aaaauuaagc ggaucauccg cuga                                            4644


<210>  4614
<211>  4677
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4614
augaagaaaa uccugggccu cgaccugggg accaacagca uuggaugggc ccuugugaau       60

gucgauaaga acggcaaucc cuucgggauc gagggacugg gcuccagaau uaucccuaug      120

gaccaggcua cacucucuga uuuugaaaaa gggaacagca agucccaaac ggccgagcgg      180

accauguucc gcggagcgag acggcugcgc gaaagaaaac accuucggcg cgagagacug      240

caucggauuc ucaaugaacu gaacuuucug ccgauccacu accgcucuug caucgacuuc      300

guucauagac ucggcaaguu uaaggcuaau cacgagccca aaauugccug gcggagcaac      360

gaucaggaca agauggaguu caucuuucaa aauuccuucc uggaaaugau gucugauuuu      420

acaaaacgcc agccugagcu ugugaaggaa ggcaaaaaga uucccuauga cuggaccauc      480

uacuuccuga gaaagaaagc ccucgggcaa cggauugaga aggaggaacu ggcuuggcuu      540

cugcuccauu uuaaccagaa acgcggauau uaccagcuga gaggcgagga ugaaaucgac      600

gccacacgga gcgcuaagac cagaaaauau uucgaucgca agauuaucaa auccaucauu      660

gacacagggg aggaauacaa gggacugaag gugcucaucg ucgagcugga uaauggcaac      720

caggggaaaa uuuuuaagca agaggugccu gacuggguug gacaggaaaa gaauaucauu      780

gccaccgugg accuugauaa ggacggcaaa gaucgguucg acaagggcgu ccugagccag      840

agauucucua uccccuccga uagcgagugg gaaacggagu ggaagcucau caaacugaag      900

accgaagagg accuugaggc gucucagaaa acagugggga ccuauauuua cgauucccug      960

cucaagaacc cuaaucagaa aaucaaggga aagcugguuc gcacaauuga acggaaauac     1020

uauagagacg agcugcgcgc cauccucaac aagcagcggg aauuucaccc ggagcugaac     1080

gauaaaaagc uguaccagca cuguauuuuc agccuuuauc cccaaaauga ggcuuuuaga     1140

aacucuaucg ccaauaaaga cuucaccuac cucuuucugg aagauaucau uuucuaucag     1200

cgcccucuua aguccaagaa aagcacgauc aacaauugcg cuuacgaggu gcggagauau     1260

auggacaagg aaacaaaaga gcugaaggaa gucccccuca aauguauugc caagucccau     1320

ccuaucuuuc aggaguuccg ccuguggcag uuuauuucua accugcggau cuacaaaaag     1380

gacauuaccg auaucgacgu gacaaaugau uuccuccaca gccaagacga uuacgcgaac     1440

cuguuugagu ggcuuaauaa caagaaugaa aucgaccaga aagccuuccu gaaguauccc     1500

gcuuuuggcu ucaaaaagga ugccgugaac uacagaugga auuaugucga ggacaaaucc     1560

uacccgugca acaagacccg gagcgcuauu cucucuaagc uggauaaaca ugaacaggag     1620

uuccugacca aggaacuuga gcucaaaauc uggcagcugc uguauuccgu uaauagcaag     1680

gaggaaauug acaaagcccu caacgugaac ucuccuaaga ucaauaacaa gauuagcauc     1740

gaucacacag ggauccugca cgagcuuaaa uccucuagcc uguccgacau uagcauccaa     1800

cgccucaagg ccauuaauuu ugaagagaaa gauuacggau cuuauuccua caaggcuauc     1860

aaaaagcugc uggcgcuuau gagacagggc aaguacuucg acaacacggc cauuaccgac     1920

gcuacaaaag cccggaucaa uagcaucuuu gauagacucg aaucuauuuc cuucgacccc     1980

gagcgcauca acagcauuuc cgaugacgau aucccuaagc agauucugaa aucuuuuauc     2040

aagcugaauc ggaccuaugg gggauucaac acauaccagg ccuguuaugc uguguacggc     2100

agacauagcg aggccaaaga aaucgcgaag ugggagaaac ccgaagacau ugaucaauau     2160

cucgcuaagu uuaagcagca cucccugcgc aaucccaucg ucgagcagau uaucaccgaa     2220

acgcuuagag ugguucggga cauuuggaaa aagcaugccu acaaaacaau caacaaggau     2280

aaucagggca acaauugcga gucugagagc gaacugaccg agauucacau cgaacucggg     2340

cgcgacauga aaaacaauaa guccgagaga cugcggauca gccgcauuaa cucugagaac     2400

gaaaauacca accugagaau ccggaaacuu cucauugagu ucaaugaucc ugaauuugga     2460

guggagaacg uccgcccgca uucccccagc caacaggaaa uccugagaau uuaugaggac     2520

ggcgugcuga aaucuggggu ugagaucccu gaauacgugg auaauaucau uaagaaauuc     2580

aaggagacag acgucaaaaa gcagcccucc aagagcgaag ugcuccggua caaacugugg     2640

cuugagcaaa aguaucgcuc uccuuacacc aacgaaguca ucccccuggg aaaacucuuu     2700

acaaaugacu aucagauuga gcacaucauu ccgcagagca gauacuucga ugacuccuuu     2760

agcaacaagg ugaucuguga ggccgaaguu aaucagcuga aagagaacga acugggcugc     2820

gaguucauca ugaauaacgg gggccggauu guggaacuuu cuuucggaaa gacggucaga     2880

auccucgauu uugaggcuua ugagcaauuc gugcagcaga auuacgccgg caaccgcccu     2940

aagcugcgga aucugcucau gaccgaaauu cccaacgacu uuaucgauag acagcugaac     3000

gacucccgcu auauuagcaa aguugugaag gggcuucugu ccaauaucgu ccgggaggau     3060

ggagaagcug agucuacaag caaaaaccuc auuuccugua ccggcggggu gacagccaga     3120

cugaagaugg acuggggacu gaaugaugug uggaacaauc uuaucuaccc ucgcuucgag     3180

cggcugaacg aacucaccaa uucuaaccaa uuuggcuacu gggacaaaaa ggaugggaag     3240

aauguuuucc agacgcaggu ccccauggac auccagaaag gcuuuaacaa gaaaagaauu     3300

gaccaccgcc aucacgcgcu ggaugcccuc gugaucgcuu gcgccacccg gagccauauu     3360

aauuaucuga acaaugaguc cgcuaagucu aacagcaaag aauccagaua cgaccuucgg     3420

cgcaagcuga gacggcucga ggugauccaa aaagaauaua caauuaaugg acgcaaggag     3480

aucagaaaca ucgaggucgc caaggaguuc uacaaaccuu ggaauagcuu uacccaggau     3540

gcgcagucug cccuggaaaa cauugugguu uccuucaagc aaaaccugcg ggugaucaau     3600

aaaacaacca acuauuuucu uagauucaau aaggaaggca aaaagauuuu cgagacgcag     3660

acaagcgggu cuaacugggc uauccgcaag ccccugcaca aagacaccgc cuuuggacuc     3720

gucaaucuga agcggacaaa aaccgugucc cucgcuaacg ccauucugga uuacgaccag     3780

aucguuaaua gaaaacuuug gaccaagauc aaggaacuga uuagcaucgg cuaugauaac     3840

aagcgcauuc ucaaauacuu cacagacgau aaggagaauu ggaacgagau cauuucuucc     3900

gggaucgaag uguuuuacua uacggacaau uucgagaaac aggucgccac ccggaagccg     3960

cuggauacca gcuuuacaga auccaaaauu aucaacagca ucacagaccu gggcauucac     4020

aagauccuuc uggcucaucu cagagcgaau gagggaaacc cuaauauugc cuucucuccc     4080

gaagggaucg agaccaugaa ccgccacauu ugugagcuga acggcggaaa guuucaucac     4140

cccaucuaca aagugcgggu ucaugaaccu cucggcuaua aguucaaagu gggggauaag     4200

ggaaauagaa gcacgaaaua cgucgaggcu gccaagggca caaaccuguu uuucgggauc     4260

uauauuaauu cccaaggaau ccgcucuuac gaaaccauuc cgcuuaacgu ggccaucgag     4320

agacagaaac agggccugag ccccgugccu gacaauaacg agcuggggga acggcuccug     4380

uuuuggcuuu cccccaauga ccuggucuau cucccuaccu cugaugaggu ugaaagcggc     4440

auuugcugca acaagcuggg aaucgaccgc aucuacaaga ugguguccug uacaggcuac     4500

gugggggauu ucauucccgc ucacaucgcc aauccuauua uccagacccu cgagcuggga     4560

ucuaacaaua aagcgcaaag agcuuggaca ggcgaaauga uuaaggagau cugcauuccc     4620

gucaaagugg accggauggg gaacaucguu gagaucuuua aucgcaacaa gaauuga        4677


<210>  4615
<211>  4695
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4615
auggccaaga uccugggccu cgaccugggg accaacagca uuggaugggc uaucguggau       60

gacaaaaauu ccaagauucu ugauacaggc gucagaaucu uccccgaggg gguuguggcc      120

aaaacgauug gaucuggcga caaggaagag agcaaaaacg cgaagcggcg cgaauccaga      180

cagacccggc gcggguuuua cagaaagcgg cugcgcaaaa ucaagcuccu gagaacacuu      240

aucaauuuca aaaugugccc ucuggagcac uuugaacucg augugugguc uaagugggac      300

aaaauuaaga agaaagaggg acggauggcu ccggagcugu uccugcccga aaacagcgcc      360

uauaaucucu ggcugaagca aaacccuuac auccuucgcg auaaagcccu guccaaggac      420

cucacccuga cagaguuugg cagaguccuu uaucaucuga uucagcggcg cggcuuccuc      480

ucuaauagaa aagggaagga ugacggaaaa aucuacggcg ggaaggaagg aauugugggc      540

aucgauaaga ccaacgagca gauuaaaaau agcacacugg ggaaguuucu guucaaaauc      600

cuccccaagg agggagaacc uuuuaaaauc auugaggacg aacugggcaa cgagcuucgg      660

guucgcucca gauauacccu gcgggauaug uacaucgcug aauuugaggc cauuuggaau      720

agacaggcua agcaccucgg ccuggacaac aucuauuaca gcaaugugaa gaacguccuu      780

cugaaagggu cucucaagaa uaaaaagaac gagcgcaaaa uuaaccaucu gaagaagauc      840

aaaggaaaug gcaacaucga aauuaucacg cugaauaaca aucucuccaa gaacgagaaa      900

cccaaguacc ugcuuaaaau ugccaccaag cugccucuca aggaauuucu ggcggggcgg      960

aucgagaaag acgaagaugg aaagauuaaa uucaagagca augagucugu gcuuuucugg     1020

caaagaccgc ugcgcuccca gaaaaagcuc cuggccaaau gucgguucga gcccgaccug     1080

aaggaugaca acggcaaguu cauccagaaa gggaagacac cuugccaccu cagccauccc     1140

cuguaugaag aguacagagc uuaucaguac guuaauaaca ucgaauaugg caaaaagcaa     1200

cgccuugagg aaucucagcg gaaacaggug auugaucuga ucaauuccaa ggacggaaac     1260

uuuaaguuca gcgagauuaa aaagaaacuc aagcuguccu acgagaccuu uaauuauucu     1320

gaugacagcu ccgucaacgg caauuacaug aucaaaaacc ugaacagcau uuucccuaag     1380

gaaauccuug agaaggaauu uguggugaau gucaacaaag aggauauuau cgaguacggg     1440

uaugaaagaa ucuggcaccu cuuccacuuu ugugaggaca augauguucu gauuaagaaa     1500

cugcaggaca acuucgaccu cucugaaaau gauaucgaga agauuuccaa aaucaaccug     1560

aaggaaggau acagcaaugu gucucuuaag gccauuaaaa acauccugcc cuaucucaag     1620

gagggcaaac ugcugagcga cgcugugauc cuugggggag uccgcaaugu guuuggcaag     1680

aaauggcaug aguucauuga uuuucacgaa aagaucgaga aagacauucu cuacaucaac     1740

caucaaaagu ccaauaagga aggggaggcc auugagaaaa ucaagaaaua ucugaucgau     1800

uacaaguucg gcuuuacaga aaacgacaau cgguucaaaa agcuguauca ccacucucag     1860

gauguugugg aggucaagcu ccagaaaaag auuggaccgg uggaagaccu gagaaacccu     1920

aucguucaac agggcgugaa ugagguccgc cggcuuguga acgaacugcu cgauguccau     1980

gggaaauucg accaggugag aguugagcug ggacgggauc ugaagaacaa uaaaagcaag     2040

cgcgaagaga uuggcuacaa gaucagagag aacaccgcga aaaaugaaaa ggccaaagag     2100

cuucucauug aauacgggcu gcggcagucc agagacaacg ugcaaaaggu ccugcucuuu     2160

caggagaugc uggaucgcgg aacgguggcu guuugccccu auacaaauaa aaccaucaac     2220

aucagcgacg ugcuuggcaa ugagaacaag augcagauug aacacaucau uccuaagucu     2280

aucucccugg acgauagcuu cgccaauaaa acacucugug acucuaaguu uaacgggcug     2340

aaaggcgagc ggacccccua cgaguucuau cagauuaaua acgaugcuaa ucugugggga     2400

ggcuccaaga gcugggaaga aaucgagaga cgcaccuuuc ggauucuucc cuacuccaaa     2460

gccaagagau ucacaucuaa gacgaaauuu gagaccaacg acuucaucca acgccagcuc     2520

aaugauacaa gauauaucag caagaaaacc aaggaaauuc ugucccagau cugcgaggac     2580

guccggguga ugccuggguc ucugacagcc gaacuccgca aacugugggg acuuaacaac     2640

auucugcauc cggcuuuugu gcucgaucug cccgcgcugc acaucgacgc caauaagcau     2700

auuccucacu acguuguccu ugaugaggac aacgugccca uccugagcca gagaaucuau     2760

aaugauaaac cuauucucaa ggcuaaccaa accuccauca cgggcuucgu ggacaaugaa     2820

auuuuuaaga gcaaagagaa guacgucaaa uucgagguga aggcccccaa ccugucuaau     2880

ggggaauacu gggccaaacu ccugcuuucc aacccgaaaa gcaucguucg gguguuuaag     2940

gagaaaccua agucuacaga aaaugagauu gucgugcgcg gaaaaaucga aaaggagaaa     3000

uucaagcacg agggccuguc cgggaucaac gcugaaggca aggagaaugg aaccuauugg     3060

gccaaacuca gcauuguuga uaagaaauuc aagaaaccca agaaggacga ccagccuaaa     3120

aagaacggca aacagauccu gcuguuuggg gcggugaagg aaggagucuu cucuagcuac     3180

auguaugagu gugaugcuga ccuugaggau ggcaaauacu ggcugauucu cgacaucgau     3240

uuugaaaaug uggaguucga aaaggcccug aacgagagac ccgaaguucu cgagaaucag     3300

auucugaucc aagggauuac caacgacgag aauguguuug ucuccgauau ggacugccag     3360

caucaguucc uuaugaacga aggaaaaggc aaguauuacg ugcuguuuga uaucaucagc     3420

gaggugaacg aguucuaucc uauuaagaca aaaucucccg aacucgaaaa ugggcaaucc     3480

cugaccgagg gcacaaucug ggucgacaag uacacgggag agauuaaauu ugauccgaag     3540

aaaaaccggg aagaccaccg ccaucacgcc aucgacgcuc ugguuauugc ccuuaccaag     3600

gagacauacu uccagcagcu gagcagacgg aaugcuuccu cugaaaacaa gaaaagaggc     3660

aaugaguaug aaaaggagca gcucgauuuu gccgagccuu ggaaagaguu ccacguggac     3720

gcgcaaaaga gcaucaacuc cauccugauu ucucauaaac agaauaagaa cauccucacc     3780

aagauuacaa agaagaucac caaaaauggg aagacguaca aaagcgaagg aauguccgug     3840

cgcggccagc ugcacgauaa gaccuauuac gggcauaaca agaauuguaa agccgacgaa     3900

uaugucuacc ggguggauau uaacaaucuu ucuuucagcc agcugacaaa caucaucgac     3960

cugaaucuca aggcuguuau uguggaggga gucaucaaaa aguuugagga auccgagaaa     4020

cugcuuuuca agaaaaugaa cagcaaggug caaaaccugg auaagucuaa aguuaagaaa     4080

auuaaggcca aaguggacaa gauccucaag gaaaccuccu uuuucaugcc caaugagggc     4140

aaaagauaua uucgccugaa caucauugaa cccgcuaauu uugagcggau gccugucccg     4200

aucaagaaag ugagaauccg caagguucuc cggaacgccg agaaacugaa uaacgaaauu     4260

aagcaguucg uggaucccca gaauaaccac cauguccuua ucuacaagga cgagaauggc     4320

aaccugaaag aagagguggu gacauuuugg accgucguug aaagaaagcg cacgggggag     4380

ccuguguaca aacugcccag cgauggaaag aaaauuguga caacccucca gaucaaugac     4440

auguuccugc uuggccugaa cgaggaugaa auuaauuggg agaacccuaa uuauaaccac     4500

cucaaggaac accuguaccg gguccaaaag uuuacaucug gggacuucua uuuuagaauc     4560

gcgaaaaccu ccagcaucca gaacggagag gaaaagcagc aaauuaauuc uuucggccuc     4620

gggaaaaacg gauggcugac ccauaauccc aucaagguga aaauuuccgu uagcggcaag     4680

auccuuaagg uguga                                                      4695


<210>  4616
<211>  4713
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4616
auggagaaca gcaauaacuc cucuaagaaa uacuaucugg gccucgacau ggggaccgau       60

agcugcggau ggugugugac agacgauaag uacaauauca uuagauccca caaaaagauc      120

uauggccccg acgggaaacu ggucaagcgg gaaggaugcc aucuuugggg cucucgccug      180

uucagaccug ccaacacggc uaagagccgg cgccucaaua gaaccgcccg gcgcagacug      240

gcgcggcgca gacggcgcau uauccuucug agagagcucu uucagccgga aauugauaaa      300

guugacccca acuucuuucg gcgccuggau caauccaauc uguggaacga ggacaaggau      360

aaagaacucc agauggagca caauuuccug uuuaacgaca aggaucuuac agacaaagag      420

uacuauaagg auuucccuac caucuaccau cugagaaagg cuauggugaa ugggcucaca      480

aucaacggau auuacuacga ccccaaaaag caguuugaca uucgggaagu guaucugguc      540

cuugcccaca ugaucaaaua cagaggcaau uuccugaugg agggcgaugu gaaggacucu      600

guuguggauc cuaccuaucu caaacagugu uuugacaaca uugaugucau ccugauuagc      660

cugaauaacg gggaagacga ugagaacgag gacgauaaga ucgaccccuc cgaaugcugu      720

uucaaugugg augagaaaaa ggcccgcaag cucgcuaaca auuuucaaga aacaaacaaa      780

guuagcgacc uggaggacga ugaacgggac cuuuucggaa aguuuaauuc uaaagauauu      840

agaaagaaaa uccugaagcu caucaacggc ggguccggag ugaagcugaa agacuuccuu      900

ccuaaggaga uucuggucgc ccaugauggc gacgaugagu acggggacaa aaagaucgau      960

cucagcucug aaacccugga ggacgauauu aauggauauu cccugccgga aagcuacgug     1020

gagaucauuc ucgaagcuaa aaagaucuau gacuucaaga cgcuggccua ccuucugcac     1080

ggcaaaucuc uccugugcga ugcgauggug aacgucuacg aggagcacaa ggaacagcuu     1140

gccgcucuga aacgccucua uaagaaauac gccaccaagg agcaguauua cuuuauguuc     1200

cgggacucca gaauccugga cggcgaugac agcgaugacg aagaggauaa ggaagagucc     1260

aauaaagagg aaucuaagga caaaaagaaa aaggcuagcu auguuuccua cauuggguau     1320

acaaaaacca acagcaaggc caaguacaca ggagcgcaua ucaccucuga gaaaaauauu     1380

uccgcccuga ucaagcagau uaaagaugac cucaaguuug augaccugaa aagcaaggug     1440

gaugaccccu cucaacagaa cgaacuuagc gaugaggaca agcuggacau ccaguguauc     1500

gaggaagcua uugaguccaa uacguuccuc ccucgccaga acucucggaa uaacggcgcc     1560

uuucccuacc aacugaaccu gaaugaaaug agaacaauca uugagaacca gagcaaauau     1620

uacccuuucc uuaaggauaa agcucccucc uuuccggacg ggaagaccga agaguauaaa     1680

aucgugagcc uccugaaguu caagauuccu uacuauaucg gaccccuguc ucagccuaau     1740

gauuacgucc gcgacgauaa acgggacaac cacugggugc aauuuagagg cgagaauaac     1800

caggggauua ucaaucccug gaacuucucc cagaucguug aucucgaagg aacagccgac     1860

aaguuuauua gcaaucugac caacuauugc accuacaucu ucggcgagaa uacacuuccc     1920

aaagaaucuc ugauuuuuca ggccuacaag gugcucaacg aucugaauaa ccugaaaguc     1980

aauggcuccc uuaucagcaa ggaggacaag gauuauauua uccaaaacgu guaccucaaa     2040

aagaaaaaga ucaaagggaa ggacauuguu aacgcucuga aaaauaagua uaagcugucc     2100

gacgaagauc cuacggugac cacaaaagga ucuggcaaga gcaucgggga ggacgagguc     2160

gaucucacca uguucaacuc cucucugagc uccuucauug acaugagcga uccgaaugcg     2220

uuuggacccg aguucuacaa agacucuaac aaguuugaac uugccgaaaa agugaucgag     2280

cugauuacaa ucgucgaaga uaaggaccuc cugaaggagc gccugaaaaa gcucggccuu     2340

uccgauaauc aggugaacua ucuguucaaa cagaaguaca gcgggugggc uccucugucu     2400

cggaaacucc uggacggcau caccauugau uguguuaaug agaacacggg agaagccuau     2460

cccgugacaa ucauugacgu caugagagau accccucuua auuuuaugga gaucuacgaa     2520

accaaggagu cccaguucag cggcuuuaag gagaaagugg ccgaacugaa cgacgagaag     2580

aaacucucuc gggacgaucu gauugaagag agcuacgcuu cccccgccau gaagcgcagc     2640

guuagacaaa cauucaaagu gaucgacgaa cugaagaaua cccuuaacau cgaucauuuu     2700

gaccggauuu ucgucgaggc gacaagagcu ccgggggagg ccggaaagau ggugaaaucu     2760

cgcaagaaac agcuccagga agccuaugau gcugccaagg accuggugga ucaggagucc     2820

cgggacgauc ugcaaaaacu cagagacgau cuggaaaagc agacggacga ucagcuucgc     2880

agaaagaaag uuuaccugua uuuuaugcag cucggccgga gcgucuacuc cggggagaag     2940

aucgaccuga augaacugga caaaaacuau gauauugacc acaucauucc ucaagcuaaa     3000

cuuaaggaug acucuuucaa uaacauggug cugguggagc gcgagcucaa caauaagaaa     3060

caggaugaau accccauccc uagcaccauu aucuccgagc acggaagaaa cuggauccag     3120

aagcuggcca agauucaaga caaaaauaag cggucuuauu uuaugcccaa agauaaggcg     3180

aaccgccucc ugagaccuga aagcaaacag cuuacauccg acgagcugaa uggcuucauc     3240

aaccggcagc ucacccugac aaaucagucu gucaaggccg ugugcgauau ucugaagaug     3300

accgaaaaag ggagcgagau cguuuacucc aaggcuaacu ucgugagcga cuuucgcaaa     3360

cuuuguggcg agcugagacc cucuuacgau gccuucguca aggugcggga cauuaaugac     3420

uuucaucacg cuaacgaugc cuaucucaau aucguugugg gaaacgucua cuaugaaaaa     3480

uucaagaauu uuuacaucaa caaucgcauu gacuaugaua acaauggcaa gaaagaccug     3540

uccgugcucg cgagcaagcu guucacgaaa acccaagccg augacaagac agggaacauc     3600

uacugggagg cuagauuuca uaacgaaaaa gagaccgauu cuaauggaaa ggagcggaca     3660

auuaagguua gagacgaagg caccgagucc acguucgaua aaaucgaaua uuacauggag     3720

cuuaacgacc cgcuggugac acagaugcag uacacccuca gcgggaagca gggauuuuuc     3780

aaaaagauua cacugaaaac caaggccaaa gauaaugaag acaacucuga uaagcuguuu     3840

ccucuuaagc aauaucugcg cuccgacagc aauaaaguca uggacgugaa caaguacggc     3900

ggguauuccg auaugaucac ccccuacuuc augcucgugc agagcgaggg caaaaaggga     3960

aaacacaucu auucucugga gagcauuccc acaaucuacc ucaaguccuu caagaaaaag     4020

gaagaggaca uuaucaauua ucugucuugc aaaaacaaac uuaagaaucc ugucguuauu     4080

agcgauaagc ugcugaugcg gacgaucgug aagauuccgu acuuuaaaaa cgacuccaau     4140

cucucucugg ugaaacuugg caccagcggg agaacaggaa acaauaucau cuacauuaac     4200

cuguccgaac ucauccugcc cauugagugg aagaauuauu accgcaagau cucuaaccuc     4260

cugggcacca acgucagcuc cacaaaaccu aaggaucuua gcaaguucac cgaacagaug     4320

aaaaaugacc ccacgucuac acugguggau ggguccaaca agguugagca ugacaaaaau     4380

aaccgguuuu ucagcuaucu gauuaauaac gugaucaaua agccuaucuu ugagggacuc     4440

cccaaaaccu cuugcuccuu cauuaagcug gaaagcaaau acgaugaguu uuguucccuu     4500

aacacccugg aacaggcuaa gaucauugcc acaaucauua agcuccuguc uugcaaaagc     4560

guccaaggcu guaagcucuc caaucugagc gggcuuccuu cuucccuggg cgcccugauc     4620

agcaacaaag cucucaccga gggaacaauc auuucuaagg aaagcuccug cggccugaaa     4680

gagaauauca uuuucguggu ucccaacgac uga                                  4713


<210>  4617
<211>  5067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4617
auggaguuca agaaagugcu gggccucgac aucgggacca acagcauugg augcgcccug       60

cuuucccugc ccaagucuau ccaggauuac ggcaaagggg gaagacucga auggcugaca      120

agccggguca uuccucuuga cgcugauuau augaaggccu uuaucgacgg caaaaauggg      180

cugccgcaag uuauuacgcc cgcgggaaag cgcagacaga agcggggcuc ccgcagacuc      240

aaacaccggu acaagcugcg cagaucucgg cugauccgcg uguucaaaac ccucaacugg      300

cugccugagg auuuuccccu ugacaauccu aagagaauca aagaaacaau uagcaccgag      360

ggcaaguucu ccuuucggau cucugauuau gugcccauua gcgacgaauc cuaccgcgag      420

uucuauagag aguuugggua cccugaaaac gagaucgaac aggucauuga ggagaucaau      480

uuccggcgca agacaaaagg aaagaacaaa aauccgauga uuaagcugcu ccccgaagau      540

ugggugguuu auuaccugag aaaaaaggcu cuuaucaaac cuaccacaaa ggaggaacug      600

auccggauua ucuaccucuu uaaccagaga cgcggcuuca agagcucucg gaaagaccug      660

accgagacgg ccauucugga uuaugacgaa uuugccaaga gacucgcuga gaaagagaag      720

uacuccgccg aaaauuauga gaccaaauuc gugagcauca caaaggucaa ggaagugguu      780

gagcugaaaa ccgaugggcg caagggaaaa aagcgguuua aagugauucu ugaagacucu      840

agaaucgagc ccuacgagau cgaacgcaag gagaagccug acugggaagg caaagaguau      900

acauuccugg ucacccaaaa gcucgaaaaa gggaaguuca aacagaacaa gcccgaucug      960

ccgaaggagg aggacugggc ucuuuguacg acagcccugg auaaucggau gggauccaaa     1020

cauccuggcg aauuuuucuu ugacgagcuc cugaaggcgu ucaaagaaaa gagaggcuac     1080

aaaauucgcc aguaucccgu gaaccggugg agauacaaga aagagcugga guuuaucugg     1140

accaagcagu gccaacucaa uccugaacug aacaaucuua acauuaacaa ggagauccug     1200

cgcaaacucg ccacagugcu guaccccagc cagucuaagu ucuuugggcc caaaauuaag     1260

gaauucgaga auuccgaugu ccuucacauc aucagcgaag acauuaucua uuaccagcgg     1320

gaucugaaau cccagaaguc ucucauuagc gaguguagau augagaagcg gaaaggaauc     1380

gacggcgaaa uuuacgggcu gaagugcauc ccuaaaucca gcccgcugua ucaagaguuu     1440

cgcauuuggc aggauaucca uaacaucaag guuauuagaa aagaaucuga ggugaaugga     1500

aagaagaaaa ucaacauuga cgaaacccag cucuacauca augagaacau uaaggagaaa     1560

cuguucgaac uuuuuaauuc caaggauagc cugucugaga aagacauccu cgaacugauc     1620

agccugaaca uuaucaauuc cggcauuaag aucucuaaga aagaggaaga gaccacacac     1680

cggauuaacc uuuucgcuaa uagaaaggag cucaaaggga acgaaacgaa gagccgcuau     1740

cggaaagugu uuaagaaacu gggauucgac ggcgaguaca uccugaauca ucccuccaag     1800

cucaacagac uguggcacag cgauuacucu aaugacuaug ccgauaagga aaaaaccgag     1860

aaguccaucc uuagcucucu gggcuggaaa aaccgcaacg ggaaguggga gaaauccaag     1920

aauuacgacg ucuucaaccu cccucuggaa guggcuaagg ccauugcgaa ucugcccccu     1980

cuuaaaaagg aguauggaag cuacuccgcc cucgcuauca gaaaaaugcu gguugugaug     2040

cgggauggca aguauuggca acaccccgac cagauugcca aagaucagga aaacacaucu     2100

cugaugcucu uugacaagaa ucugauccag cuuaccaaca aucaacgcaa gguccugaac     2160

aaauaccucc ugacacuggc ugaggugcag aagagaagca cccuuauuaa acagaagcuc     2220

aaugaaaucg agcauaaccc guauaaacug gagcugguuu ccgaucagga ccucgaaaag     2280

caagugcuga agucuuuccu ugagaaaaag aaugaaagcg auuaccugaa agggcucaag     2340

acguaccagg ccggauaucu gaucuacggc aaacacuccg agaaggacgu cccuauugug     2400

aacagccccg augaacuggg ggaguauauc cggaagaaac uuccuaauaa cucucuccgc     2460

aaucccauug ucgagcaggu gaucagagaa acaauuuuua ucguucggga cguguggaag     2520

uccuucggca uuaucgacga gauccauauu gaacugggac gcgagcugaa aaacaacagc     2580

gaagagagaa agaaaaccuc ugagucccag gaaaagaauu uucaagagaa agaacgggcc     2640

cgcaagcucc ugaaggagcu ucugaacagc ucuaauuucg agcacuacga ugaaaacggc     2700

aauaaaaucu uuagcuccuu caccgucaac ccuaaucccg acagcccgcu cgauauugag     2760

aaguuuagaa ucuggaaaaa ccagucuggg cugacagacg aagagcugaa uaagaaacuu     2820

aaggaugaaa agauuccuac cgagaucgag gugaaaaagu auauccugug gcucacacag     2880

aaaugucggu cccccuacac gggaaagauu aucccccuga gcaaacucuu cgacuccaac     2940

guuuaugaaa uugagcacau cauuccuaga ucuaagauga agaaugauag caccaacaau     3000

cuggugaucu gcgaacuugg cgucaacaaa gcuaaggggg accgccuggc ggccaauuuu     3060

aucuccgagu cuaacggaaa auguaaguuc ggcgaagugg aguacacacu ccugaaauac     3120

ggggauuauc ugcaguacug caaggacacc uuuaaguauc aaaaagcuaa guacaaaaac     3180

cuucuggcca cagagccgcc cgaagauuuc auugagcggc agaucaauga caccagauau     3240

auuggccgca agcucgccga acugcucacg ccugugguua aagauagcaa gaacaucauu     3300

uucaccaucg gauccauuac aucugagcug aaaaucaccu ggggccuuaa uggggucugg     3360

aaggacaucc ugcggcccag auuuaagcgc cucgaaagca uuaucaacaa aaagcugauu     3420

uuccaggacg aggaugaccc uaauaaauac cauuuugauc uguccaucaa cccccaacuu     3480

gacaaggagg gacugaaacg gcucgaucac agacaucacg cucuggacgc cacaauuauc     3540

gcggcuacca cgcgcgaaca ugugcgguau cucaauagcc ugaacgccgc cgauaaugac     3600

gaggaaaaga gagaguacuu ccuuucucug uguaaccaca agauccggga uuuuaaacug     3660

ccuugggaga auuucacauc cgaagugaag agcaaacucc ugucuugcgu cguguccuac     3720

aaggagagca aacccauucu uucugacccg uuuaacaagu aucugaagug ggaauacaaa     3780

aauggcaagu ggcagaaagu uuucgcuauc cagauuaaga acgaucgcug gaaagccgug     3840

agacggucca uguuuaagga gccuaucggg accgucugga uuaagaaaau caaggaagug     3900

agccucaaag aggcuaucaa gauucaggcc aucugggagg aaguuaaaaa ugaccccgug     3960

cgcaagaaaa aggagaagua uauuuacgac gauuaugcgc aaaaagucau cgccaagauu     4020

gugcaggaac ugggacucuc cagcucuaug agaaaacagg acgaugagaa gcugaacaaa     4080

uucaucaaug aagcuaaggu uagcgccggc gugaacaaga accuuaauac aaccaacaaa     4140

accauuuaca aucuggaggg gcgguuuuau gagaagauca aagucgcuga auacgugcug     4200

uacaaggcca aaagaaugcc ucucaacaag aaggaguaua ucgaaaaacu gucccuucag     4260

aagauguuca augaccugcc caacuucauu cucgagaaau cuauccugga uaauuacccg     4320

gaaauucuca aggagcugga gagcgacaac aaauauauca uugaaccuca caagaagaau     4380

aaccccguga aucgccuucu gcuggagcau auccucgaau accacaacaa uccuaaagag     4440

gcguuuucca cagagggccu ggaaaagcuu aacaaaaagg ccaucaauaa aauuggaaag     4500

cccaucaagu auauuacgcg gcuggauggc gacaucaacg aggaagagau uuucagaggg     4560

gcugucuuug aaaccgauaa aggaucuaac guuuacuucg ugauguauga gaauaaccaa     4620

acaaaggacc gcgaguuucu caaacccaau ccuagcaucu ccgugcugaa ggccaucgaa     4680

cauaaaaaca agauugauuu cuuugcuccg aaucggcucg gcuucucuag aaucauucug     4740

agccccgggg accuugucua cgugccuacc aacgaucagu acguucugau caaagacaau     4800

uccagcaacg agacaauuau caauugggac gauaacgaau uuauuucuaa ucgcaucuau     4860

caggugaaga aguucaccgg aaacuccugu uacuuucuga aaaaugacau cgccagccuc     4920

auucugucuu auuccgccag caacggcguc ggggaguucg gcucccagaa uaucucugaa     4980

uacagcgugg augacccccc uauucggauc aaggauguuu gcauuaaaau cagaguggac     5040

cgccuuggaa acgucagacc ccuguga                                         5067


<210>  4618
<211>  3924
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4618
augacccagu ucgagggcuu uacaaaccug uaccaaguga gcaagacgcu cagauucgaa       60

cugauccccc aggggaaaac ccuuaagcac auucaggagc agggauuuau cgaagaggac      120

aaagcccgga augaucauua uaaggaacug aaaccuauua ucgaccgcau uuacaagaca      180

uaugcugauc aaugccucca gcugguccag cuugacuggg agaaccuguc cgccgcgauc      240

gauucuuaca gaaaggagaa aaccgaagag acacggaaug cucucaucga ggaacaggcc      300

accuaucgca acgccauuca cgacuacuuc aucggcagaa cagauaaucu gaccgacgcu      360

auuaacaagc ggcaugccga gaucuauaaa gggcuguuua aggcugaacu cuucaaugga      420

aaaguucuga agcaacuugg cacggugacc acaaccgagc acgaaaacgc ccugcuccgc      480

agcuuugaua aguucacaac cuacuuuucc ggguucuacg agaauagaaa gaacguguuu      540

ucugcggaag acauuagcac ggccaucccg caucggauug uccaggauaa uuuccccaag      600

uuuaaagaga acugucacau cuucacacgc cugaucaccg cugugccuuc ccuuagagag      660

cacuucgaaa auguuaagaa agccauugga aucuuugugu cuacaagcau ugaggaaguc      720

uucuccuuuc ccuucuauaa ccagcugcuc acccagaccc aaaucgaccu guacaaucag      780

cugcucggcg gcauuagccg ggaggcuggg acagaaaaga ucaaaggacu gaacgaggug      840

cuuaaccugg ccauccagaa gaaugaugag acggcgcaua uuaucgccuc ucucccucac      900

cgcuuuauuc cccuguucaa gcaaauccuu uccgacagaa acacccugag cuuuauucuc      960

gaagaguuca aaucugacga agagguuauc caguccuuuu gcaaguauaa aacacugcug     1020

cggaaugaaa acgugcucga gaccgcugag gcccuguuca augaacuuaa cagcaucgau     1080

cugacacaua uuuuuaucuc ucacaagaaa cucgagacca uuuccagcgc ucugugugac     1140

cacugggaua cgcuucgcaa ugcccuguac gaaagacgga ucuccgagcu cacaggcaag     1200

auuaccaagu cugccaaaga gaagguccag agaagccuga aacaugaaga caucaaccug     1260

caggagauua ucuccgcugc ggggaaggaa cucagcgagg ccuucaaaca aaagaccucu     1320

gaaauccugu cccacgcuca ugccgcccuu gaucagccuc ugccgacaac ccucaagaaa     1380

caggaggaga aggaaauucu gaaaagccag cuggacucuc uucucggacu guaucaccuu     1440

cucgauuggu uugcugugga cgagagcaau gaaguggauc ccgaguucuc cgcccgccug     1500

acaggcauca aacuugaaau ggagccuucu cugagcuucu acaacaaagc gcggaauuau     1560

gcuacgaaga agcccuacuc cgucgagaaa uuuaagcuca acuuccaaau gccuacccug     1620

gccagcgggu gggacguuaa uaaagaaaag aacaauggag ccauucuguu ugugaaaaac     1680

ggccuuuacu aucucggcau caugcccaag cagaaaggga gauacaaggc ucugucuuuc     1740

gagccgacag aaaagaccuc cgagggauuu gauaaaaugu auuacgacua uuucccugac     1800

gccgcuaaga ugauucccaa augcagcaca cagcugaagg ccgugaccgc gcauuuucag     1860

acgcacacca caccuauccu ccugucuaac aauuucaucg aaccccuuga gauuaccaaa     1920

gagaucuacg aucugaacaa ucccgaaaag gagccuaaga aauuucaaac agccuaugcu     1980

aagaaaaccg gcgaccagaa gggguaccgc gaagcccucu guaaauggau ugauuucacg     2040

cgggacuuuc uguccaagua cacaaagacc acaagcaucg aucuguccuc ucuuagaccg     2100

agcucccagu auaaagaccu cggagaguac uaugcugagc ugaacccccu gcucuaccac     2160

auuucuuucc agcgcaucgc cgaaaaggag aucauggaug cggucgaaac cggcaaacug     2220

uaucuuuuuc aaauuuacaa uaaggacuuc gccaaagggc aucacggaaa gccuaaccug     2280

cauacccucu auuggacagg ccuguucagc cccgagaauc uggcuaagac guccaucaaa     2340

cuuaacggcc aggccgaacu cuuuuaccgg ccuaagagca gaaugaaacg cauggcucac     2400

cggcuggggg agaagaugcu gaauaaaaag cucaaagauc agaagacccc cauuccggac     2460

acacuguacc aagagcuuua ugauuacgug aaccacagac ugucucauga ccucuccgau     2520

gaagcccgcg cccugcugcc uaauguuauc accaaggagg ugagccacga aauuaucaaa     2580

gaccggagau ucacaucuga caaguuuuuc uuucaugucc ccaucacccu uaacuaucag     2640

gcugccaauu ccccuagcaa auucaaccag cgggugaaug cguaccugaa ggagcacccc     2700

gaaacgccua uuaucggaau ugaucgcggc gagagaaacc ucaucuauau uacaguuauc     2760

gacucuaccg ggaaaauucu ggagcagcgg agccucaaca ccauccaaca guuugauuac     2820

cagaagaagc uggacaauag agaaaaagag cgcguggcug cccggcaggc cugguccguc     2880

gugggaacaa ucaaggaucu uaaacaaggc uaucugagcc aggucauuca ugaaaucgug     2940

gaccucauga uucacuacca ggcuguugug guccuggaga accugaauuu cggguuuaag     3000

ucuaaaagaa ccggcaucgc cgaaaaggcg guguaccagc aauucgagaa gaugcuuauu     3060

gauaaacuga acugccucgu ucugaaggac uaucccgcug agaaaguggg aggcguccuc     3120

aauccguacc agcugacaga ucaguuuacg uccuucgcca agauggggac ccaaagcgga     3180

uuccuguuuu augugccugc ccccuacaca uccaaaaucg acccccugac cggcuucgug     3240

gauccuuuug uuuggaagac aaucaagaac cacgaaucuc gcaaacauuu ccucgagggg     3300

uuugacuucc ugcacuauga ugucaagacc ggagacuuua uucugcauuu caaaaugaau     3360

agaaaccuua gcuuucagcg gggccugccg ggguucaugc ccgcuuggga caucguguuu     3420

gaaaagaaug agacgcaguu cgaugccaaa ggcaccccuu uuauugcggg aaagcgcauc     3480

gugcccguca uugagaacca cagauucaca ggccgguacc gcgaccucua uccugcuaau     3540

gaacugaucg cccuccugga ggaaaagggg aucguguuca gagauggauc caacauucuu     3600

cccaaacugc uggagaauga cgauucucau gccaucgaca ccaugguugc ucucauucgg     3660

agcgugcugc agaugcgcaa cuccaaugcc gcuacaggcg aagauuacau caacucuccu     3720

gucagagacc uuaacggggu guguuuugau agccgguucc aaaaucccga guggccgaug     3780

gacgccgaug cgaacggagc cuaccacauu gcucugaagg gccagcuccu gcucaaucac     3840

cugaaagagu ccaaggaccu uaaacugcag aacgggauca gcaaucagga cuggcuggcc     3900

uauauucaag aacuccgcaa cuga                                            3924


<210>  4619
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4619
augagcaucu accaggaguu cgugaacaag uauucccugu cuaaaacccu cagauuugaa       60

cugauucccc aaggcaagac acuugagaau aucaaagccc gggggcugau ucucgacgau      120

gaaaagcgcg cuaaagacua caagaaggcc aaacagauca uugauaagua ucaccaguuc      180

uuuaucgagg aaauccugag cuccgucugc auuucugagg accuucugca gaacuacagc      240

gauguuuauu ucaaacucaa gaaauccgac gaugacaauc ugcaaaagga uuuuaagucu      300

gcgaaagaca cgaucaagaa acagauuagc gaguacauca aggauuccga aaaauucaag      360

aaccuguuua aucagaaccu cauugacgcu aaaaagggac aggagagcga ucugauccuu      420

uggcugaagc aaucuaaaga caauggcauu gaacucuuca aggccaacuc cgacaucacc      480

gauaucgacg aggcccugga gauuaucaag agcuuuaagg gguggacaac cuauuucaaa      540

ggauuucaug aaaauagaaa gaacguguac ucuuccaaug auauuccuac aagcaucauu      600

uaccggaucg uggacgauaa ccuuccgaag uuccuggaga auaaagcuaa guaugaaucu      660

cucaaagaca aggcccccga ggcuaucaac uacgaacaga uuaaaaagga ucuggccgag      720

gagcugaccu uugacaucga uuauaagaca uccgaaguca aucagcgcgu guucagccuc      780

gacgagguuu ucgaaauugc gaacuuuaac aauuaccuga accaguccgg caucaccaaa      840

uucaauacga uuaucggggg aaaguuugug aacggcgaga auaccaaaag aaagggcauc      900

aacgaauaua uuaaucuuua cucucaacag aucaacgaua aaacacugaa gaaguauaaa      960

augagcgucc ucuucaagca gauucugucc gacaccgaga gcaaaucuuu ugugaucgac     1020

aaacuugagg augacuccga uguugugaca accaugcaga gcuucuacga acaaauugcc     1080

gcuuuuaaaa cggucgagga aaagucuauc aaagagacac ugagccuccu guucgacgau     1140

cugaaggccc agaagcucga ccuguccaaa auuuacuuua agaaugauaa aucucuuacc     1200

gaccugagcc agcaaguguu cgaugacuau uccgugaucg ggacagcugu ccucgaauac     1260

aucacccagc agauugcccc uaagaaccug gauaauccca gcaaaaagga gcaggagcuu     1320

aucgcgaaga aaaccgaaaa ggccaaauau cugucucucg agacaauuaa gcuggcucug     1380

gaagaguuua acaaacaccg ggacaucgau aagcaauguc gcuucgagga aauucucgcc     1440

aauuuugcug ccaucccuau gaucuucgac gagauugccc agaacaagga caaucuggcu     1500

cagaucucca uuaaauacca gaaccaagga aagaaagauc uucugcaggc gagcgccgaa     1560

gacgauguua aggcuaucaa agaccuccug gaucagacga acaaucugcu ucauaagcuc     1620

aagauuuucc acaucucuca guccgaggac aaagccaaca uccuggauaa ggacgaacau     1680

uuuuaucugg uguucgagga gugcuacuuu gaacucgcca auauugugcc ccuguauaac     1740

aaaaucagaa auuacauuac ccaaaagccu uacagcgaug agaaauucaa acuuaacuuu     1800

gaaaauucca cacuggcuaa cggcugggac aagaauaagg agccggauaa caccgccauc     1860

cucuucauua aggacgauaa auauuaccug ggggucauga auaagaaaaa caauaagauc     1920

uuugacgaca aagcgaucaa ggaaaacaag ggagagggcu auaaaaagau uguguacaaa     1980

cugcuucccg gggcuaauaa gaugcucccu aaaguuuucu uuucugccaa gagcaucaag     2040

uucuauaacc ccuccgagga uauucugcgg auccgcaacc acucuacaca caccaaaaau     2100

ggaagcccuc agaagggcua cgaaaaauuu gaguucaaca uugaagacug uagaaaguuu     2160

aucgauuucu auaaacaguc cauuagcaag caucccgagu ggaaggacuu cggcuuucgg     2220

uucucugaua cgcaacgcua caauuccauc gacgaauuuu acagagaggu ggagaaccag     2280

ggguauaaac ugacauucga aaauaucagc gagucuuaca uugauuccgu cgugaaccag     2340

ggaaagcucu aucuguuuca gaucuacaau aaagacuuca gcgccuauuc uaagggccgg     2400

ccgaaccuuc acacccugua cuggaaagcu cucuuugaug aacgcaaucu gcaagacguu     2460

guguauaagc ugaacgggga ggccgagcuu uucuacagaa agcagagcau uccuaaaaag     2520

aucacccauc ccgcuaaaga agccauugcg aauaagaaca aagauaaucc uaagaaagag     2580

uccgucuuug aauacgaccu caucaaggac aagcgguuca cagaggauaa auuuuucuuc     2640

cacugcccca ucaccauuaa cuuuaagagc ucuggagcca auaaauucaa cgacgaaauc     2700

aaccugcugc ucaaggagaa agcuaaugau gugcacauuc uguccaucga cagaggcgag     2760

cgccaucuug ccuauuacac acuggucgau gggaagggaa acauuaucaa gcaggacacg     2820

uuuaauauca uuggcaacga ucggaugaaa accaauuauc acgacaagcu cgcugccauc     2880

gaaaaagaua gagacagcgc gcgcaaggau uggaaaaaga uuaacaauau caaggagaug     2940

aaagaaggcu accuguccca ggugguucau gagauugcca agcuggugau cgaauauaac     3000

gcuauugucg uguucgagga ccuuaauuuu ggguucaaac ggggaagauu uaagguugag     3060

aaacaagugu accagaagcu cgaaaagaug cugaucgaga aacugaacua ucucgucuuc     3120

aaggauaaug aauuugacaa aacaggcggg gugcugcgcg ccuaccagcu uaccgcuccc     3180

uucgagacau uuaagaaaau gggaaagcag accggcauca uuuacuaugu gccugccggg     3240

uucacgucua aaaucugucc gguuaccggc uuugucaacc aacuguaccc caaguaugaa     3300

agcgugucca agucucagga guucuucagc aaauuugaca agauuugcua caaucucgau     3360

aaaggauauu ucgaguuuuc cuucgacuac aagaacuuug gcgauaaagc cgcuaagggg     3420

aaguggacaa ucgcgucuuu cggaagccgg cugauuaauu uuagaaacuc cgacaaaaau     3480

cacaacuggg auacccgcga aguguauccu acaaaggagc uggaaaaacu ucugaaggac     3540

uacagcaucg aguacggcca uggggagugu aucaaagccg cuauuugcgg agaaucugau     3600

aagaaguucu uugccaaacu caccuccguc cugaacacga uccuccagau gcggaauagc     3660

aagacaggca ccgagcugga cuaucuuauu ucucccgugg ccgauguuaa cgggaauuuc     3720

uuugacucca gacaagcucc uaaaaacaug ccccaggaug ccgacgcgaa uggcgcuuac     3780

cacaucggac ugaagggccu caugcugcug gggcgcauua aaaacaauca ggaaggaaag     3840

aaacuuaacc uggugaucaa gaaugaggaa uauuucgagu uuguccagaa ccggaauaac     3900

uga                                                                   3903


<210>  4620
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4620
augaagcccg acaucaaaga uagagaguac auguaucggc ugauuaucgg ccaacucuuc       60

uacgacgggc accagcagau ugccgugaac cuggcuaaua cccuuggaug cagcgccccu      120

ccgcccccuc ccuccgauaa gcuguuucgc cucgucacaa ucgcgaaaca guucguugaa      180

gacccugauu auaaggcuga ggaaaacacg augcccaugc aguuugagac cucuagcgcc      240

ggccuggacc uucaauacga ugccgacgug gcugccacau ccccugaacc gucucaguau      300

gagaccauuu accugacagu gcauaaagcu cccuguagag ccgcggccuu caauagcgau      360

gggucccuca ucgcuaccgg aucugccgac ugcagcauca agauuaugga ugucgagcgg      420

auccuggcuc gcgaaaagga gcacagagaa augaacgaga auggcccuga cgcccaucac      480

cccgugauuc ggacacugua ugaucauguu gacgugcgca gagacuuugg gcgguucucc      540

gauaaaaucc ucgacuuuca ggaugucaac accgugauuu uccacccucg cgacgcgauc      600

cugauuagcg gaucuaauga uaagacgguu aaacuuuuug acuucuccaa gaccgccgug      660

aaaagagcua ugaagacacu gagcgagguc uaccccgugc gggcccucuc uugucacccg      720

ggcggcgaau uucugcuugu ggcuaccgau cauccuacag uccgccugua caacaucgag      780

accgcccagg ccuaugcuuc cgcgaauccc gacgaucaac acagagaaag cguuacggac      840

gugcauuacu cugagaacgc ccggcucuau gugacagcuu ccaaggaugg ggacgucaaa      900

gugugggacg gaguuagcuc ccgcugcgug gaaaccuuca agagagccca cgauggcucu      960

agcaucuguu ccgccaaauu uacaaagaau gggaaauaca uucugaccag cggaauggac     1020

ucuaucguca agcuguggga gcucuccacc aaccggugcc ugauugugua uacaggcgcu     1080

ggggccacgg gagcgcagga uuacccuacc agcgcuucuu ucaaucagaa cgaggacuuc     1140

guucuuuuuc ccgaugagaa aagcggcucc auguguucuu gggaagccag aaaugccgac     1200

cgcaagcggc ugcucgcucu gggccacaca agcgccugca gaaccuucgu gcauuccccc     1260

agcaugccug cuuuuaugac agggucugau gaccaccgcg cccgguucug guauagaaag     1320

ccgaccacga acgauaacug a                                               1341


<210>  4621
<211>  1293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4621
augaagcccg acaucaaaga uagagaguac auguaucggc ugauuaucgg ccaacucuuc       60

uacgacgggc accagcagau ugccgugacc cuggcuaaca cacuuggaug cagcgccccu      120

gcgccgcccc cuuccgauaa gcuguaucgc cucgucacga ucgcuaaaca guuuguugaa      180

gaccccgauu acaaggccga ggaaaaaucu cugccuaugc aguucgagac cgugagcaca      240

ggccuugacc uggaauauga ugccgacgug caacccgucu ccccugagcc gucucaguac      300

gagaccauuu aucucacagu gcauaaggcu cccuguagag ccgcugccuu uaauagcgau      360

gggucccugg uugcgaccgg aucugccgac ugcagcauca agaucaugga uguggaacgg      420

auucuggcuc gcgagaaaga acacagagag augaacgaga auggcccuga cgcccaucac      480

cccgucaucc ggacacucua cgaucaugug gacgacguua acaccgugcu guuccacccu      540

cgcgaugcua uucuuaucuc cgggagcaau gacaagacgg ucaaacuguu ugauuucucu      600

aagaccgccg ugaaaagagc gaugaagaca cucuccgaag uguaccccgu ccgggcccug      660

agcugucacc cgggaggcga guuucuucug guugcuaccg accauccuac agugcgccuc      720

uauaacauug aaaccgccca ggcuuacgcc ucugccaauc ccgaugacca gcacagagag      780

uccgugacgg auguccauua uagcgaaaac gcucggcugu acgugacagc gucuaaggac      840

ggcgauguua aaaucuggga cggggugucc aaucgcugcg ucgagaccuu caagagagcc      900

cacgauggaa gcgcuauuug uuccgccaaa uuuacaaaga acggcaaaua ucugcucacc      960

ucugggaugg acagcaucgu gaagcugugg gagcuuucca ccaaucggug ccugaucguu     1020

uacacaggag ccggcgcuac gggggcccaa gacuaugcga ccagcgcuuc uuucaaccac     1080

aaugaagauu acgugcucuu uccugacgag aaauccggaa gcauguguuc uugggaugcc     1140

cgcaacagcg acagaaagcg gcugcuugcc cugggccaua cauccgcuug cagaaccuuc     1200

guccacucuc ccagcaugcc cgccuuuaug acaggcuccg augaccaucg cgcucgguuc     1260

ugguacagaa agccugccac cacggauaau uga                                  1293


<210>  4622
<211>  3690
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4622
auggccgcua gcaagcugga gaaauucacc aacugcuacu cccucucuaa gacacugaga       60

uuuaaagcca uccccguggg caagacgcag gaaaauauug acaacaaacg gcuucugguc      120

gaggaugaaa agcgcgcgga ggacuauaag gggguuaaaa agcuccugga uagauacuau      180

cuuagcuuca ucaaugacgu gcugcacucc auuaaacuca agaaccugaa uaacuacauc      240

ucucuguuuc ggaaaaagac ccgcacagaa aaggagaaua aagagcucga aaaccuggag      300

auuaaucuua gaaaggaaau cgcuaaagcc uucaagggag ccgcuggcua uaaaagccug      360

uuuaagaaag auaucauuga gaccauccuc ccugaggccg cugacgauaa ggacgaaauu      420

gcccugguga acuccuucaa uggguuuaca accgcguuca caggauuuuu cgauaaccgg      480

gagaauaugu uuucugaaga ggccaagagc accuccaucg cuuuccgcug uauuaacgaa      540

aaucuuacga gauacaucag caacauggac auuuuugaga aagucgaugc caucuucgac      600

aagcaugagg ugcaagaaau caaagagaag auucugaacu cugauuauga cguugaagac      660

uucuuugagg gcgaguucuu uaauuucgug cucacccagg aagggaucga ugucuacaac      720

gcuauuaucg gaggcuuugu gacagagucc ggcgaaaaga uuaaggggcu gaaugaguac      780

aucaaccugu auaaugccaa gaccaaacag gcgcucccga aguucaaacc ccuguacaag      840

cagguucuua gcgaccggga aucucugucc uuuuauggag agggcuacac aagcgaugaa      900

gaggugcucg aggucuuccg caacacccug aauaaaaacu cugaaaucuu uuccagcauu      960

aagaaacuug agaaacuguu caagaauuuu gacgaauauu ccucugccgg gaucuucgug     1020

aaaaacggac cugcuauuag cacgaucucc aaggauauuu uuggcgagug gaaucucauc     1080

agagacaaau ggaacgccga guacgaugac auccaccuga agaagaaagc uguggucaca     1140

gaaaaguaug aggaugaccg gcgcaagagc uucaagaaaa uugggucuuu cucccuggaa     1200

caacuccagg aguacgccga ugccgaccug agcguugugg aaaaacuuaa agagaucauu     1260

auccagaagg uggacgagau uuacaagguc uauggaucua gcgaaaaacu guuugaugcu     1320

gacuucgugc ucgagaaguc ccugaaaaag aaugaugcgg uuguggccau caugaaagac     1380

cuucuggauu cugucaagag cuuugaaaac uacauuaagg cuuucuuugg cgagggcaaa     1440

gaaaccaaua gagacgaguc cuucuauggg gauuuugugc ucgccuacga cauccugcug     1500

aagguugauc auaucuauga cgccauucgg aacuacguga cacagaaacc cuauagcaag     1560

gauaaauuca agcucuacuu ucaaaacccu caguucaugg gaggcuggga caaggauaaa     1620

gagaccgacu accgcgcuac cauccugaga uaugggucua aguacuaucu ugccauuaug     1680

gacaaaaagu acgcgaaaug ccugcagaag aucgauaagg acgaugucaa uggaaacuau     1740

gaaaagauua auuacaagcu ccugcccggc ccuaacaaaa ugcugccgaa gguguuuuuc     1800

uccaaaaagu ggauggcuua uuacaauccc agcgaggaca uccagaaaau cuacaagaac     1860

gggacauuua agaaaggaga uauguucaau cuuaacgacu gucacaagcu cauugauuuc     1920

uuuaaagacu cuaucucccg guauccuaag uggagcaaug ccuacgauuu caacuuuucc     1980

gaaacggaga aauauaagga cauugccggc uucuacagag aagucgagga gcaaggcuau     2040

aaggugucuu uugaaagcgc uuccaaaaag gagguugaua aacuggugga agaggggaag     2100

cuguacaugu uccagaucua uaauaaagac uuuucugaua agagccaugg aacccccaac     2160

cuccacacaa uguacuucaa gcugcuuuuu gacgagaaua accacggcca gauucgccug     2220

uccgggggag ccgaacucuu caugcggaga gcuagccuga aaaaggagga acuggucgug     2280

cauccugcca auucucccau cgcgaacaaa aacccggaca auccuaagaa aaccacaacc     2340

cuuuccuacg auguuuauaa ggacaagcgc uuuagcgagg aucaauacga acuccacauc     2400

cccauugcca ucaacaaaug cccuaagaau auuuucaaaa ucaacacgga ggugcggguc     2460

cugcugaagc augacgauaa ucccuaugug auuggcaucg acagagggga gcgcaaccuc     2520

cuguacaucg ugguugucga uggcaaagga aauauugugg aacaguauuc ucuuaacgag     2580

aucauuaaua acuucaaugg cauccggauu aagacagacu accacucccu gcucgauaag     2640

aaagaaaagg agagauuuga agcucgccag aacuggacca gcaucgagaa uauuaaagag     2700

cugaaggccg gguauaucuc ucaggugguc cacaaaaucu gugaacuggu ggagaaguac     2760

gacgcuguua uugcccuuga agaucucaac agcggauuca aaaauucccg ggugaagguc     2820

gagaagcaag uguaccagaa auuugaaaag augcugaucg acaaacugaa cuauaugguu     2880

gacaagaaaa gcaaccccug cgcgaccggc ggggcccuca agggauacca gauuacaaau     2940

aaguucgagu cuuuuaaauc caugagcacc cagaacggcu ucaucuuuua uauuccugcu     3000

uggcugacau ccaagaucga uccgucuacg ggguucguga aucuucugaa aaccaaguac     3060

acaagcaucg ccgacuccaa aaaguuuauu ucuagcuucg auagaaucau guaugucccc     3120

gaggaagacc ucuuugaguu cgcucuggau uacaagaacu uuucccgcac cgacgccgau     3180

uauauuaaaa aguggaaacu guacucuuac ggcaaucgga ucagaauuuu cgccgcugcg     3240

aagaaaaaca auguguucgc cugggaagag guuugucuua caagcgcuua uaaggagcuc     3300

uuuaacaagu acggaaucaa uuaucaacag ggcgacaucc gggcccugcu gugcgaacag     3360

uccgauaaag ccuucuacag cucuuuuaug gcucucaugu cccugaugcu ucagaugcgc     3420

aacagcauua ccgggagaac ggacguggau uuccugaucu cuccugucaa aaauuccgac     3480

ggaaucuuuu augauagccg gaacuacgag gcccaagaaa augcgauucu ccccaaaaac     3540

gcugacgcca auggcgccua uaacauugcu agaaaggugc ugugggccau cgggcaguuc     3600

aaaaaggcgg aggacgaaaa acuggauaag gugaagauug cuaucucuaa uaaagagugg     3660

cuugaguacg cccagaccuc cgucaaguga                                      3690


<210>  4623
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4623
augagcaucu accaggaguu cgugaacaag uauucccugu cuaaaacccu cagauuugaa       60

cugauucccc aaggcaagac acuugagaau aucaaagccc gggggcugau ucucgacgau      120

gaaaagcgcg cuaaagacua caagaaggcc aaacagauca uugauaagua ucaccaguuc      180

uuuaucgagg aaauccugag cuccgucugc auuucugagg accuucugca gaacuacagc      240

gauguuuauu ucaaacucaa gaaauccgac gaugacaauc ugcaaaagga uuuuaagucu      300

gcgaaagaca cgaucaagaa acagauuagc gaguacauca aggauuccga aaaauucaag      360

aaccuguuua aucagaaccu cauugacgcu aaaaagggac aggagagcga ucugauccuu      420

uggcugaagc aaucuaaaga caauggcauu gaacucuuca aggccaacuc cgacaucacc      480

gauaucgacg aggcccugga gauuaucaag agcuuuaagg gguggacaac cuauuucaaa      540

ggauuucaug aaaauagaaa gaacguguac ucuuccgaug acauuccuac aagcaucauu      600

uaccggaucg uggaugacaa ucuuccgaag uuccuggaga acaaagcuaa guaugaaucu      660

cucaaagaua aggcccccga ggcuaucaau uacgaacaga uuaaaaagga ccuggccgag      720

gagcugaccu uugauaucga cuauaagaca uccgaaguca accagcgcgu guucagccuc      780

gaugagguuu ucgaaauugc gaauuuuaac aacuaccuga aucaguccgg caucaccaaa      840

uucaacacga uuaucggggg aaaguuugug aauggcgaga acaccaaaag aaagggcauc      900

aaugaauaua uuaaccuuua cucucaacag aucaaugaca aaacacugaa gaaguauaaa      960

augagcgucc ucuucaagca gauucugucc gacaccgaga gcaaaucuuu ugugaucgau     1020

aaacuugagg acgauuccga cguugugaca accaugcaga gcuucuacga acaaauugcc     1080

gcuuuuaaaa cggucgagga aaagucuauc aaagagacac ugagccuccu guucgaugac     1140

cugaaggccc agaagcucga ucuguccaaa auuuacuuua agaacgacaa aucucuuacc     1200

gaucugagcc agcaaguguu cgacgauuau uccgugaucg ggacagcugu ccucgaauac     1260

aucacccagc agguugcccc uaagaaucug gacaacccca gcaaaaagga gcaggaucuu     1320

auugcgaaga aaaccgagaa ggccaaauau cugucucucg aaacaaucaa gcuggcucug     1380

gaggaauuua auaaacaccg ggacauugac aagcaauguc gcuucgagga gauccucgcc     1440

aacuuugcug ccauuccuau gaucuucgau gaaaucgccc agaauaagga caaccuggcu     1500

cagauuuccc uuaaauacca gaaucaagga aagaaagauc ugcuccaggc gagcgccgag     1560

gaagacguga aggcuaucaa agaucugcug gaccagacga acaaccuucu ccauagacug     1620

aagauuuucc acaucucuca guccgaggau aaggccaaua uucuggacaa agaugaacau     1680

uuuuaucucg uguucgagga gugcuacuuu gaacuggcca acaucguccc ccuuuauaau     1740

aagauccgga acuacauuac ccaaaaaccu uacagcgacg agaaguucaa acugaauuuu     1800

gaaaacucca cacucgcuaa uggcugggau aagaacaaag agccggacaa uaccgccauc     1860

cuguucauua aggaugacaa guauuaccug ggggugauga acaaaaagaa uaacaaaauc     1920

uuugacgaua aggcgauuaa agaaaauaag ggagagggcu auaagaaaau cguuuacaaa     1980

cuucuccccg gggcuaacaa gaugcugccu aagguguucu uuucugccaa aagcaucaag     2040

uucuauaauc ccuccgagga cauucugcgc aucagaaacc acucuacaca caccaagaac     2100

ggaaauccuc agaaaggcua cgaaaaguuu gaguucaaca uugaagauug ucggaaauuu     2160

aucgacuucu auaaggagag cauuuccaaa caucccgaau ggaaggauuu cggcuuucgc     2220

uucagcgaca cgcagagaua caauucuauc gaugaguuuu accgggaggu cgaaaaccaa     2280

ggguauaagc ucacauucga gaauauuucc gaaagcuaca ucgacucugu gguuaaccag     2340

ggaaaacugu aucuuuuuca gaucuacaau aaggauuucu ccgccuauag caaaggccgc     2400

ccgaaccugc acacccucua cuggaaggcu cuguuugacg agagaaaucu gcaggaugug     2460

gucuauaaac uuaacgggga ggccgaacuc uucuaccgga agcaaucuau uccuaagaaa     2520

aucacccauc ccgcuaagga ggccauugcg aauaaaaaca aggacaaucc uaaaaaggaa     2580

agcguguuug aguacgaccu gaucaaagau aagagauuca cagaagacaa guuuuucuuc     2640

cacugcccca uuaccaucaa cuuuaaaucc agcggagcca auaaguucaa cgaugagauc     2700

aaccugcucc ugaaagagaa ggcuaaugac guccacauuc uuucuaucga ucgcggcgaa     2760

cggcaucugg ccuauuacac acucguggac gggaaaggaa acauuaucaa gcaggauacg     2820

uuuaauauua ucggcaacga cagaaugaag accaauuauc acgauaaacu ggcugccauc     2880

gagaaggacc gcgauuccgc gcggaaagac uggaagaaaa uuaacaauau caaggaaaug     2940

aaggagggcu accugagcca gguugugcau gaaauugcca aacuugucau cgagcacaac     3000

gcuauugugg uuuucgagga ucucaauuuu ggguucaaga gaggacgcuu uaaaguggaa     3060

aagcaggucu aucaaaaacu ggagaagaug cugaucgaaa agcucaacua ccugguguuc     3120

aaagacaaug aguuugacaa gacaggcggg gugcuucggg ccuaucagcu gaccgcuccc     3180

uucgaaacau uuaaaaagau gggaaaacag accggcauua ucuacuacgu uccugccggg     3240

uucacgucca agaucugucc ggucaccggc uuugugaacc agcucuaucc caaauacgag     3300

ucugugagca agucccaaga guucuucucu aaguuugaua aaauuugcua uaaucuggac     3360

aagggauacu ucgaauuuag cuucgauuau aaaaacuuug gcgacaaggc cgcuaaaggg     3420

aaguggacaa ucgcguccuu cggaucuaga cugauuaauu uucgcaacag cgauaagaau     3480

cauaacuggg acacccggga ggucuacccu acaaaagaac uugagaagcu gcucaaagau     3540

uauuccaucg aguacggcca cggggaaugu auuaaggccg cuaucugcgg agagagcgac     3600

aaaaaguucu uugccaagcu gaccucugug cucaacacga uccugcagau gagaaauucc     3660

aaaacaggca ccgaacuuga uuaccugauu agccccguug ccgacgugaa cgggaauuuc     3720

uuugauucuc gccaggcucc uaagaacaug ccccaagacg ccgaugcgaa uggcgcuuau     3780

cacaucggac ucaaaggccu gaugcugcuu gaccggauua agaacaauca ggaggggaaa     3840

aagcugaacc ucgucaucaa gaaugaagag uacuucgagu uugugcagaa cagaaauaac     3900

uga                                                                   3903


<210>  4624
<211>  1071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4624
augaagacca acuaccacga caaacuggcc gcuaucgaga aggauagaga aagcgcccgg       60

aaagacugga agaaaauuaa uaacaucaag gagaugaagg aaggcuaucu cucccaggug      120

guccaugaga uugcgaaacu gguuaucggg uacaaugcua uugugguguu cgaagaucuu      180

aacuuuggau ucaagcgcgg cagauuuaaa gucgagaagc aaguguauca gaaacuggag      240

aagaugcuca ucgaaaagcu gaauuaccuu guuuucaaag acaacgaguu ugauaaggcc      300

gggggagugc ugcgggccua ucagcucaca gcucccuucg aaacguuuaa aaagaugggc      360

aaacagaccg ggaucauuua cuaugucccu gccgacuuca caucuaagau cugcccggug      420

accggauuug uuaaucaacu guaccccaaa uacgagagcg uguccaaguc ucaggaguuc      480

uuuagcaagu ucgauaaaau uuguuauaac cuggacaagg gcuacuuuga guucuccuuc      540

gauuauaaaa auuuuggcga caaggcugcc aaagggaagu ggacaaucgc gucuuucgga      600

agccgccuca uuaacuuuag aaauuccgau aagaaccaca auugggacac ccgggaaguc      660

uacccuacaa aagaacugga gaaacuucug aaggauuaua gcaucgaaua cggccauggg      720

gagugcauua aggccgcuau cuaugccgag aacgacaaaa aguucuuugc uaagcucacc      780

ucuauccuua auuccauucu ucagaugcgc aacagcaaaa cgggaaccga acuggacuac      840

cucaucucuc ccguggccga ugugaacggc aauuucuuug acuccagaca cgcgccuaag      900

aacaugcccc aggaugccga cgcuaauggg gccuaccaua uuggacugaa aggccugaug      960

cuccuguauc ggaucaagaa caaucaagau ggcaaaaagc ugaaccuggu cauuaagaau     1020

gaggaauacu ucgaguuugu ucagaaccgc aauaaaagcu cuaagaucug a              1071


<210>  4625
<211>  3792
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4625
auggaggacu acagcggcuu cgugaacauc uauuccauuc agaagacccu gagauuugaa       60

cucaaacccg ucgggaagac acuggagcac aucgagaaaa agggauuccu uaaaaaggau      120

aagauucggg ccgaggacua caaagcuguu aagaaaauca uugauaagua ucaucgcgcc      180

uacaucgaag agguguuuga cucugugcug caccagaaaa agaagaaaga uaagacgaga      240

uucagcaccc aauuuaucaa agagauuaag gaguucuccg agcucuauua caaaacagaa      300

aagaauaucc cugacaaaga gcggcuggag gcgcuuucug aaaagcugcg caagaugcuc      360

gucggcgcuu uuaaagggga guucagcgaa gagguggccg aaaaguauaa aaaccuguuu      420

uccaaggagc ugauuagaaa ugagaucgaa aaauucugcg agaccgauga ggaacggaag      480

cagguuucua acuuuaagag cuucacaacc uacuuuacag gauuccauuc caaucgccag      540

aacauuuaca gcgacgagaa aaagucuacc gccaucggcu auagaauuau ccaccaaaau      600

cucccgaaau uccuggauaa ccuuaagauc auugaaucca uccagcggcg cuuuaaagac      660

uuccccugga gcgaucugaa gaagaaucuc aaaaagauug acaaaaacau caagcugacg      720

gaguacuuuu cuauugaugg guucgugaau guccuuaacc agaaaggaau cgacgcuuau      780

aauaccaucc ugggcggcaa guccgaagag agcggggaaa agauucaggg acucaacgag      840

uacaucaauc uguauagaca gaaaaacaac auugaccgga agaaucugcc uaacgugaaa      900

auccucuuua agcaaauucu gggcgaucgc gaaacaaaau cuuucauccc cgaggccuuu      960

ccugacgauc aguccguucu uaauagcauc accgaguucg cuaaguaccu gaaacucgac     1020

aagaagaaaa aguccauuau cgccgaacug aaaaaguuuc uuucuagcuu caacagauau     1080

gagcuggaug ggauuuaccu cgcgaaugac aacucccugg ccagcaucuc uacauuucug     1140

uucgaugacu gguccuuuau uaaaaagagc gugucuuuca aguacgauga aagcgucgga     1200

gaccccaaaa agaaaaucaa guccccucuc aaauaugaga aggagaagga aaaauggcug     1260

aagcagaaau acuauaccau uucuuuccuu aaugaugcua ucgagagcua cuccaagagc     1320

caagacgaaa aacgggugaa gauccgccug gaggccuauu uugcugaguu caagucuaaa     1380

gacgaugcca agaaacaguu ugaccuccug gaaagaauug aggaagcgua cgccaucgug     1440

gagccgcuuc ugggcgcuga auauccccgg gauagaaacc ucaaggccga caaaaaggag     1500

gucgggaaaa uuaaggauuu ccuggacucc aucaagagcc ugcaguuuuu ccucaaaccu     1560

cugcuuucug cugaaauuuu ugaugagaag gaccugggau ucuacaauca acucgagggc     1620

uacuaugaag agaucgauuc caucggccau cuguacaaca aaguucgcaa uuaucugacg     1680

gggaagauuu acagcaaaga aaaguuuaaa cuuaacuucg aaaauuccac acuccugaaa     1740

ggaugggacg aaaaccggga gguggccaau cuguguguga ucuuuagaga ggaucagaag     1800

uauuaccucg gcgucaugga caaagaaaac aacaccauuc ugucugauau ccccaaggug     1860

aaaccuaaug agcuuuucua ugaaaagaug guuuacaagc ugauucccac accgcacaug     1920

cagcucccuc gcaucaucuu uagcuccgac aaccugucua uuuacaaucc cagcaaaucc     1980

auccugaaga uucgggaggc caagagcuuc aaggagggga aaaacuucaa acuuaaggac     2040

ugccacaaau uuaucgauuu cuauaaggaa ucuauuucca aaaaugagga cuggagcaga     2100

uuugauuuca aguuuucuaa aaccuccagc uacgaaaaca ucucugaguu cuaucgcgaa     2160

guggagcggc agggauacaa ucucgacuuu aagaagguca gcaaauucua uaucgauucc     2220

cugguggagg acggcaagcu guaccucuuu caaauuuaua acaaagauuu cagcaucuuu     2280

ucuaagggga aaccuaaucu gcauaccauu uacuucagau cccuuuucag caaggaaaac     2340

cugaaagacg uuugucucaa gcugaaugga gaggcugaaa uguuuuuccg caagaaaucc     2400

aucaacuacg augagaagaa aaagcgggaa ggccaccauc ccgagcuguu ugagaaacuu     2460

aaguauccca uucucaagga caaaagauac ucugaagaua aguuccaguu ucaucugccu     2520

aucagccuga auuucaaauc caaggagcgc cucaacuuua aucugaaagu gaacgaguuc     2580

cuuaagcgga acaaggacau uaauaucauc ggcauugaua gaggggaacg gaaccugcuc     2640

uaucugguca ugaucaauca gaaaggagaa auucugaagc agacacuucu cgacucuaug     2700

caaagcggca aagggcgccc ggagaucaac uacaaggaga aacugcagga aaaggagauu     2760

gaaagagaca aggcgcggaa auccugggga acgguggaga auaucaagga gcugaaagaa     2820

ggcuaucucu cuaucgucau ucaccagauc agcaagcuga ugguggagaa caaugccauu     2880

guugugcuug aagaucugaa caucggguuu aaaagaggcc gccagaaggu cgagcggcaa     2940

guguaccaga aauucgaaaa gaugcucauu gacaagcuga auuuucuggu uuucaaagag     3000

aacaagccca ccgagccugg aggcgugcuu aaggcuuauc agcugacaga ugaauuucag     3060

uccuucgaga agcucagcaa acaaaccggg uuccuguuuu acguccccuc uuggaauaca     3120

uccaagaucg acccuagaac cggauucauc gauuuucucc accccgccua cgaaaacauu     3180

gagaaggcca aacaguggau caauaaguuc gacagcauuc gcuuuaacuc uaaaauggau     3240

ugguucgaau uuacggcuga cacaagaaag uucuccgaga aucugaugcu uggcaaaaac     3300

cggguguggg ugaucugcac caccaacguu gagcgcuauu uuacaagcaa gaccgccaau     3360

ucuuccauuc aguacaacag cauccaaauu acagaaaagc ugaaagaacu cuucgucgau     3420

aucccguuuu ccaaugggca ggaccugaag ccugaaaucc ugcggaaaaa cgaugcggug     3480

uucuuuaaga gccuucuguu cuauauuaaa acgacccucu cucugagaca gaauaacgga     3540

aagaagggcg aggaagagaa agacuucauc cucagccccg uggucgacuc caaggggcgc     3600

uuuuucaauu cucuggaggc uagcgaugac gaaccuaaag augccgacgc caacggcgcu     3660

uaccacauug cccuuaaggg acugaugaau cuccuggugc ugaacgagac aaaagaagag     3720

aaucuuucca gacccaagug gaaaaucaag aacaaggauu ggcuggaguu uguuugggaa     3780

cggaaucgcu ga                                                         3792


<210>  4626
<211>  2820
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4626
augaaccugu acagcaaucu caccaacaag uauucccugu cuaaaacacu uagauucgag       60

cugauccccc agggcgaaac gcucgagaau auuaaggccc gggggcugau ccuugacgau      120

gaaaaacgcg cuaaggacua caaaaaggcc aagcaaauua ucgauaaaua ucaccaguuu      180

uucauugagg aaauccugag cuccgugugc aucucugagg accuccugca gaacuacagc      240

gaugucuauu uuaagcugaa aaaguccgac gaugacaauc uccagaaaga uuucaagucu      300

gcgaaggaca ccauuaaaaa gcauaucagc agauacauua acgauuccga gaaauuuaag      360

aaucuguuca accaaaaucu uaucgacgcu aaaaagggac aggaaagcga ucugauucuc      420

uggcugaaac agucuaagga caacggcauc gagcuuuuua aggccaauuc cgacauuaca      480

gauaucgacg aagcccugga gaucauuaag agcuucaagg gguggaccac auauuuuaaa      540

ggauuccacg agaaccggaa gaauguuuac ucuuccgaug acaucccuac cagcauuauc      600

uaccgcauug uggaugacaa ccucccgaaa uuuaucgaaa auaaggcuaa guaugagaac      660

cugaaagaua aggcccccga agcuaucaac uacgagcaga uuaaaaagga ccuggccgag      720

gagcucacau ucgauaucga cuauaaaacc ucugagguga aucaaagagu cuuuucccug      780

gaugaagugu ucgagauugc gaacuucaau aacuaccuua aucagagcgg caucacgaag      840

uuuaacacca uuaucggggg aaaguucguu aauggcgaaa acacaaaacg gaagggcauc      900

aaugaguaua uuaaccugua cucccagcag aucaaugaca aaacccucaa gaaauauaag      960

augucugugc uguuuaagca aauucuuagc gacacagaau ccaagagcuu cgucaucgau     1020

aaacuggagg acgauucuga cgugguuacc acgaugcagu ccuuuuacga gcagauugcc     1080

gcuuucaaaa cacucgaaga aaagagcauc aaagaaaccc ugucucugcu cuuugaugac     1140

cugaaggccc aaaaacuuga ucugagcaag auuuacuuca agaacgacaa aucccucaca     1200

gaucugucuc agcaggucuu ugacgauuau agcgugaucg ggaccgcugu gcuugaguac     1260

aucacccagc aaguggcccc uaagaaucug gacaaccccu ccaaaaagga acaggaucuc     1320

auugcgaaaa agacagagaa ggccaaauau cugagccugg agacgaucaa gcucgcucug     1380

gaagaguuca auaaauaccg cgacauugac aagcagugua gauuugaaga gaucuucgcc     1440

ucuuuugcug auauuccugu ccuuuucgac gagaucgccc agaacaaaaa caaucuggcc     1500

caaaucucca uuaaguauca gaaccaggga aagaaagauc uccugcagac cagcgcugaa     1560

guugacguga aggcgaucaa agaucugcuu gaccaaacaa auaaccuccu gcauaagcug     1620

aaaauuuucc acaucaccca gucugaggau aaggccaaua uucucgacaa ggaugaacau     1680

uuuuaccugg uguucgacga gugcuauuuu gaacuugcua acaucgucgc ccuguacaau     1740

aaaauccgga acuacauuac acagaagccc uauuccgaug agaaauucaa gcucaauuuu     1800

gagaacagca cccuggccaa uggcugggac aaaaacaagg aaccugauaa uacggcuauc     1860

cuguucauua aagacgacaa guacuaucuu ggggugauga acaagaaaaa uaacaagauc     1920

uuugaugaca aagccauuaa ggagaacaaa ggagaaggcu acaagaaggu uguguauaaa     1980

cuccugccgg gggcgaauaa gaugcugccc aaagucuucu uuuccgcuaa gucuaucaac     2040

uucuacaauc cuagcgagga uauccuccgc auuagaaacc acuccacaca caccaaaaau     2100

ggaucucccc agaagggcua ugaaaagcug gaguuuaaca ucgaggacug ucggaaauuc     2160

auugauuuuu acaagcauag caucucccgc cacccugaau ggaaagacuu cggcuucaga     2220

uuuagcgaua ccaagaaaua caauucuauu gacgaguucu aucgggaagu ggagaaccaa     2280

ggguacaaac uuacauuuga aaauaucucc gagagcuaua uugauucucu gguugacgag     2340

ggaaagcucu accuguucca gaucuauaac aaagauuuuu ccguguacag caagggcaaa     2400

cccaaucugc auacccuuua uuggaaggcc cucuucgacg aacgcaaccu gcaggacguc     2460

guguacaaac ugaaugggga ggccgaacuc uuuuacagaa agcaaucuau cccgaagaaa     2520

auuacacacc cugcuaagga ggccaucgcu aacaaaaaua aggauaaccc caaaaaggag     2580

agcauuuucg aauaugaccu gaucaaagau aagcgguuua cggaggacaa guucuuuuuc     2640

cacugcccua uuaccaucaa cuucaaaucc agcggagcca auaaguuuaa cgaugaaauc     2700

aaucuucugc ucaaagagaa ggcgaacgac guccauauuc ugucuaucga uagaggcgaa     2760

cgccaccugg ccuacuauac acuuguggac gggaaaggaa auauuaucug uaagaacuga     2820


<210>  4627
<211>  3795
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4627
augcuguucc aggaguuuac ccaccucuac ccccugagca agacagugag auucgaacuu       60

aaaccuaucg gcaagacgcu ggagcauauu cacgccaaag acuuucucuc ccaagaugaa      120

accauggcug acauguauca gaaggucaaa gccauccugg augacuacca ucgggauuuc      180

auugcggaca ugaugucugg gguugugcuu acaaagcugg cugaguuuua ugaaguguac      240

cucgcccugc gcaagacccc gaaagaugac agccugcaga agcagcucac agagauccaa      300

accgcccuga gaaaagagau ugucaagccc aucggauccg gcgggaaaua uaaggcugga      360

uacgaucggc uuuucggcgc caagcuguuu aaagacggga aggaacucgg agaucuggcu      420

aaauucguga ucacacagga gggcgaaucu agcccuaaac uugcccacau ugcgcauuuu      480

gagaaauucu ccaccuauuu uacgggcuuc cacgacaacc gcaagaauau guacucugau      540

gaggacaaac acaccgccau cgcuuacaga cugauucaug aaaaccuccc ccgguuuauc      600

gacaaucugc agauucuggc cacaaucaag cagaagcaca gcgcucucua ugaucaaauu      660

guuaacgagc ugaaugccaa cgggcuugac gugucccugg cgagccaucu cgauggauac      720

cacaaacugc uuacccagga aggcaucaca gccuauaaua cccugcucgg gggaaucucu      780

ggcgaggcug ggucccgcaa gauucaggga aucaacgaac ugauuaauau ccaccauaac      840

cagcacugcc agaaaagcga gagaauugcc aagcugcggc cucuccauaa acaaauccug      900

ucugacggca ugggcgucuc cuuccuuccc agcaaguucg cugaugacuc ugaggugugu      960

caggccguua augaauuuua ccgccacuau gcccaugugu ucgcuaaggu ccaaucccug     1020

uuugauagau ucgacgauua ccggaaagac gggaucuaug uggagcacaa gaaccucaau     1080

gaacugagca aacaggcguu uggagauuuc gcccuucugg gccgcgugcu cgacggguac     1140

uacgucgacg uugugaaccc ugaguuuaau gaaagauucg cuaaggccaa aacggauaac     1200

gccaaggaga agcugacaaa agagaaggac aaauuuauua agggagugca cucccuggcu     1260

ucucucgaac aggccaucga gcauuauauu gcgggccacg augacgaaag cguccaggcu     1320

gggaaacugg gacaauacuu caagcauggc cuugccggcg uggauaaccc gauccagaag     1380

auucacaauu cccacagcac caucaaaggg uuucuggagc gggaacgccc cgccggagag     1440

agagcucucc cuaagaucaa aucugacaag ucccccgaga ugacacagcu gcggcagcug     1500

aaagaacuuc ucgauaacgc ccugaauguu gugcauuucg cuaagcugcu caccaccaaa     1560

acaacgcugg acaaccaaga uggcaauuuu uauggggagu ucggagcccu uuacgacgaa     1620

cuggcgaaga uugccacccu cuauaacaag gucagagauu accugagcca gaaaccuuuc     1680

ucuacagaga aguauaaacu gaauuuuggc aaccccaccc uucucaaugg gugggaccug     1740

aacaaggaga aagauaauuu cggcgugauc cugcagaagg acggaugcua cuaccucgcu     1800

cugcuugaca aggcccacaa aaagguuuuu gauaacgcuc cgaaugccgg caaaagcgug     1860

uaucagaaga uggucuacaa acugcucccu gggcccaaca agaugcugcc uaagguguuc     1920

uuugcgaaau ccaaucugga cuauuacaac cccucugccg aacuucucga uaaguaugcu     1980

caaggaacac auaaaaaggg caacaauuuc aaccugaaag acugucacgc ccugauugau     2040

uucuucaagg cuagcaucaa uaagcauccc gaguggcagc acuuuggguu cgaauuuucc     2100

ccuaccagcu cuuaccagga ccucuccgau uucuaucgcg aggucgaacc gcagggauac     2160

caagugaaau uuguugacau uaacgccgag uacaucgaug agcuggugga acagggccag     2220

cuuuaucugu uccaaauuua caauaaggac uucagcccca aagcccacgg gaagccuaac     2280

cuccauacgc uguauuuuaa agcucuguuc ucugagaaua accuugcgaa ucccaucuac     2340

aagcucaacg gcgaagccga gaucuuuuau cggaaggcuu cccuggauau gaaugaaaca     2400

accauucaca gagccggaga gguccuggag aacaagaauc cugacaaccc caagaaacgc     2460

caguucgugu acgauaucau uaaggacaaa cgguauaccc aggacaaguu uaugcuccau     2520

guuccgauca caaugaauuu cggcgugcag gggaugacca uuaaagaauu uaacaagaag     2580

gucaaccaaa gcauccagca guacaaugau gugaacguga ucggaauuga cagaggcgag     2640

cgccaucugc uuuaccugac aguuaucaau uccaaagggg aaauucucga gcagcggucu     2700

cugaacgaua ucauuacgac cagcgccaau ggaacacaaa ugaccacacc uuaucacaag     2760

auccuggaca aaagagaaau cgagcgccuu aacgcucggg ucggcugggg cgagauugaa     2820

accaucaagg agcugaaauc cggguaccuc ucucacgugg ugcaccagau uagccagcug     2880

augcucaagu auaaugccau cgucgugcug gaagaucuua acuucggauu uaagagaggc     2940

cgcuucaaag uugagaagca gauuuaccaa aauuuugaga acgcgcugau caaaaagcuc     3000

aaucaucugg cucugaaaga caaggccgau gacgaaaucg gguccuauaa gaacgcccuu     3060

cagcugacga auaacuucac cgaucucaaa agcauuggaa agcagacagg cuuccuguuu     3120

uacgugcccg cuuggaauac cucuaaaauc gacccugcca caggguucgu cgaucuccug     3180

aagccccggu augagaacau ugcgcaaucc caggccuuuu ucggaaaauu ugacaagauc     3240

uucuacaacg cugacaaggg cuacuuugaa uuuuaugugg auuacgccaa auuuaccgac     3300

aaggcuaaaa auagcagaca gacguggguu auuugcucuc acggggauaa gcgguaugug     3360

uacgacaaaa cagccaacca gaauaagggc gcuaccaucg gaauuaacgu caaugaugag     3420

cuuaaauccc uguucgcccg cuaucauauc aacgacaagc aaccuaaucu cgugauggau     3480

aucugucaga acaaugacaa ggaauuucac aaaagccuga uguaccugcu uaaggcgcug     3540

cucgcccuga gauauucuaa cgcuagcucc gaugaggacu ucauucucag ccccguugcc     3600

gaugacaaag gcguguuuuu caauucugcu cuggccgaug acacacagcc gcagaacgcc     3660

gacgcuaaug gggcguacca caucgcccuu aagggacugu ggcugcucaa cgagcugaaa     3720

aauuccaacg aucuugacaa gauuaagcug gcuaucgaua aucaaaccug gcucaacuuc     3780

gcccagaacc gguga                                                      3795


<210>  4628
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4628
augagcaucu accaggaguu cgugaacaag uauucccugu cuaaaacccu cagauuugaa       60

cugauucccc aaggcaagac acuugagaau aucaaagccc gggggcugau ucucgacgau      120

gaaaagcgcg cuaaagacua caagaaggcc aaacagauca uugauaagua ucaccaguuc      180

uuuaucgagg aaauccugag cuccgucugc auuucugagg accuucugca gaacuacagc      240

gauguuuauu ucaaacucaa gaaauccgac gaugacaauc ugcaaaagga uuuuaagucu      300

gcgaaagaca cgaucaagaa acagauuagc gaguacauca aggauuccga aaaauucaag      360

aaccuguuua aucagaaccu cauugacgcu aaaaagggac aggagagcga ucugauccuu      420

uggcugaagc aaucuaaaga caauggcauu gaacucuuca aggccaacuc cgacaucacc      480

gauaucgacg aggcccugga gauuaucaag agcuuuaagg gguggacaac cuauuucaaa      540

ggauuucaug aaaauagaaa gaacguguac ucuuccgaug acauuccuac aagcaucauu      600

uaccggaucg uggaugacaa ucuuccgaag uuccuggaga acaaagcuaa guaugaaucu      660

cucaaagaua aggcccccga ggcuaucaau uacgaacaga uuaaaaagga ccuggccgag      720

gagcugaccu uugauaucga cuauaagaca uccgaaguca accagcgcgu guucagccuc      780

gaugagguuu ucgaaauugc gaauuuuaac aacuaccuga aucaguccgg caucaccaaa      840

uucaacacga uuaucggggg aaaguuugug aauggcgaga acaccaaaag aaagggcauc      900

aaugaauaua uuaaccuuua cucucaacag aucaaugaca aaacacugaa gaaguauaaa      960

augagcgucc ucuucaagca gauucugucc gacaccgaga gcaaaucuuu ugugaucgau     1020

aaacuugagg acgauuccga cguugugaca accaugcaga gcuucuacga acaaauugcc     1080

gcuuuuaaaa cggucgagga aaagucuauc aaagagacac ugagccuccu guucgaugac     1140

cugaaggccc agaagcucga ucuguccaaa auuuacuuua agaacgacaa aucucuuacc     1200

gaucugagcc agcaaguguu cgacgauuau uccgugaucg ggacagcugu ccucgaauac     1260

aucacccagc agguugcccc uaagaaucug gacaacccca gcaaaaagga gcaggaucuu     1320

auugcgaaga aaaccgagaa ggccaaauau cugucucucg aaacaaucaa gcuggcucug     1380

gaggaauuua auaaacaccg ggacauugac aagcaauguc gcuucgagga gauccucgcc     1440

aacuuugcug ccauuccuau gaucuucgau gaaaucgccc agaauaagga caaccuggcu     1500

cagauuuccc uuaaauacca gaaucaagga aagaaagauc ugcuccaggc gagcgccgag     1560

gaagacguga aggcuaucaa agaucugcug gaccagacga acaaccuucu ccauagacug     1620

aagauuuucc acaucucuca guccgaggau aaggccaaua uucuggacaa agaugaacau     1680

uuuuaucucg uguucgagga gugcuacuuu gaacuggcca acaucguccc ccuuuauaau     1740

aagauccgga acuacauuac ccaaaaaccu uacagcgacg agaaguucaa acugaauuuu     1800

gaaaacucca cacucgcuaa uggcugggau aagaacaaag agccggacaa uaccgccauc     1860

cuguucauua aggaugacaa guauuaccug ggggugauga acaaaaagaa uaacaaaauc     1920

uuugacgaua aggcgauuaa agaaaauaag ggagagggcu auaagaaaau cguuuacaaa     1980

cuucuccccg gggcuaacaa gaugcugccu aagguguucu uuucugccaa aagcaucaag     2040

uucuauaauc ccuccgagga cauucugcgc aucagaaacc acucuacaca caccaagaac     2100

ggaaauccuc agaaaggcua cgaaaaguuu gaguucaaca uugaagauug ucggaaauuu     2160

aucgacuucu auaaggagag cauuuccaaa caucccgaau ggaaggauuu cggcuuucgc     2220

uucagcgaca cgcagagaua caauucuauc gaugaguuuu accgggaggu cgaaaaccaa     2280

ggguauaagc ucacauucga gaauauuucc gaaagcuaca ucgacucugu gguuaaccag     2340

ggaaaacugu aucuuuuuca gaucuacaau aaggauuucu ccgccuauag caaaggccgc     2400

ccgaaccugc acacccucua cuggaaggcu cuguuugacg agagaaaucu gcaggaugug     2460

gucuauaaac uuaacgggga ggccgaacuc uucuaccgga agcaaucuau uccuaagaaa     2520

aucacccauc ccgcuaagga ggccauugcg aauaaaaaca aggacaaucc uaaaaaggaa     2580

agcguguuug aguacgaccu gaucaaagau aagagauuca cagaagacaa guuuuucuuc     2640

cacugcccca uuaccaucaa cuuuaaaucc agcggagcca auaaguucaa cgaugagauc     2700

aaccugcucc ugaaagagaa ggcuaaugac guccacauuc uuucuaucga ucgcggcgaa     2760

cggcaucugg ccuauuacac acucguggac gggaaaggaa acauuaucaa gcaggauacg     2820

uuuaauauua ucggcaacga cagaaugaag accaauuauc acgauaaacu ggcugccauc     2880

gagaaggacc gcgauuccgc gcggaaagac uggaagaaaa uuaacaauau caaggaaaug     2940

aaggagggcu accugagcca gguugugcau gaaauugcca aacuugucau cgagcacaac     3000

gcuauugugg uuuucgagga ucucaauuuu ggguucaaga gaggacgcuu uaaaguggaa     3060

aagcaggucu aucaaaaacu ggagaagaug cugaucgaaa agcucaacua ccugguguuc     3120

aaagacaaug aguuugacaa gacaggcggg gugcuucggg ccuaucagcu gaccgcuccc     3180

uucgaaacau uuaaaaagau gggaaaacag accggcauua ucuacuacgu uccugccggg     3240

uucacgucca agaucugucc ggucaccggc uuugugaacc agcucuaucc caaauacgag     3300

ucugugagca agucccaaga guucuucucu aaguuugaua aaauuugcua uaaucuggac     3360

aagggauacu ucgaauuuag cuucgauuau aaaaacuuug gcgacaaggc cgcuaaaggg     3420

aaguggacaa ucgcguccuu cggaucuaga cugauuaauu uucgcaacag cgauaagaau     3480

cauaacuggg acacccggga ggucuacccu acaaaagaac uugagaagcu gcucaaagau     3540

uauuccaucg aguacggcca cggggaaugu auuaaggccg cuaucugcgg agagagcgac     3600

aaaaaguucu uugccaagcu gaccucugug cucaacacga uccugcagau gagaaauucc     3660

aaaacaggca ccgaacuuga uuaccugauu agccccguug ccgacgugaa cgggaauuuc     3720

uuugauucuc gccaggcucc uaagaacaug ccccaagacg ccgaugcgaa uggcgcuuau     3780

cacaucggac ucaaaggccu gaugcugcuu gaccggauua agaacaauca ggaggggaaa     3840

aagcugaacc ucgucaucaa gaaugaagag uacuucgagu uugugcagaa cagaaauaac     3900

uga                                                                   3903


<210>  4629
<211>  3756
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4629
augaaccaca ugaagcaguu caccaaucaa uuuagccugu ccaaaacacu cagauucgag       60

cugauccccc agggcaagac gaaagaauuu auugagauca acgggcuuau ugaaaaggac      120

aaugagcggg ccgugucuua caaaaagguc aagaaaauca uugaugaaua ucauaaguac      180

uucaucgaga ugguucugug cgacuuuaaa cuccacggac uggagaccua ugaaacaauc      240

uucaacaaga aagagaagga ugacaccgau aagaaagaau uugacaauau ucgcaacagc      300

cuuagaaagc agaucgcuga ugccuucgcg aaaaauccua acgacgagau uaaggagcgg      360

uuuaaaaauc uguucgcuaa ggaacucauc aaacaggauc ugcugaacuu uguggacgau      420

gagcaaaagg aacucgugaa ugaguucaag gacuuuacaa ccuacuucac aggcuuccau      480

cagaaccgca gaaauaugua ugucgccgau gaaaaagcca ccgcuauugc cuaccggcug      540

gugaacgaga aucuuccgaa guuuaucgac aaccugaaaa uuuaugagaa gaucaaaaag      600

gacgcucccg aacucaucuc cgaucugaac aagacgcuug uugagaugga agagauugug      660

caggggaaaa cccuggacga aaucuucucu cucagcuuuu ucaaucagac acugacccaa      720

acaggaauug agcuguacaa caucgucauu ggcgggcgca ccgccgauga gggaaagacg      780

aaaaucaagg gccucaauga auacaucaac acagacuaua aucagaaaca gaccgauaag      840

aagaaaaagc aggcgaaauu uaagcaacug uacaaacaga uucuuuccga cagacacucu      900

gugagcuucg uugccgagac auuugaaacc gaugcucagc ugcucgagaa caucgaacaa      960

uucuauucca gcgugcugug uaauuacgag gacgauggcc auaccacaaa cauuuuugag     1020

gccaucaaga aucuuauuau cgggcugaag acguucgacc ucucuaaaau cuaucugcgg     1080

aacgauaccu cccugacaga cauuagccag aagcucuuug gagacugguc uaucauuucc     1140

agcgcucuga augauuacua ugaaaaacag aacccuaucu cuuccaagga gaaacaggaa     1200

aaguacgacg agcgcaaagc caaguggcuu aagcaagauu ucaauauuga gaccauccag     1260

acagcgcuga acgaaugcga cagcgagauu aucaaagaaa agaauaacaa aaacaucguc     1320

uccgaguacu uugccaagcu cggccuggau aaagacaaua agauugaucu ucugcagaag     1380

auccaccaca acuauguggu gauuaaagac cuccugaaug aacccuaccc ugagaacauc     1440

aagcugggga aucagaaaga gcaagucucu cagauuaagg auuuccucga cagcauccug     1500

aaccuuaucc auuuccugaa accccucucc cugaaggaua aggacaaaga aaaggaugag     1560

cuguuuuaua gccuuuucac cgcucucuuu gaacaccugu cucagacgau uuccaucuac     1620

aauaaaguua gaaacuaucu gacacagaag gccuacagca ccgagaaaau uaagcucaau     1680

uucgaaaacu cuacccugcu uaauggaugg gacgugaaca aggagccugu gaauacaagc     1740

gucaucuuuc ggaaaaacgg ccuguucuau cucgggauua uguccaaguc uaauaaccgc     1800

aucuuugaga gaaacgugcc gguuuguaaa aaugaagaga ccgcuuucga aaagaugaac     1860

uacaaacugc ugcccggagc caauaagaug cuuccuaaag uguuucucag cgccaagggc     1920

aucgaguccu uccaacccag cgcugaaauu cagucuaagu accagaaaga gacacauaag     1980

aaaggcgacg cguuuguccg gaaggauaug gagaaccuga ucgacuucuu uaaacagucc     2040

auugccaagc acacggauug gaagcacuuc aaucaucaau ucagcaaaac cgaaacauau     2100

aacgaccugu cugaguuuua caaggaagug gagaaacagg gguauaagcu caccuucaca     2160

aaacuggaug agaccuacau caaucagcuu guugacgaag gaaagcugua ucucuuucaa     2220

auuuacaaca aggauuucuc cccuuuuagc aaaggcaagc ccaauaugca cacgcuguau     2280

uggaaaaugc uguucgacga gcagaaccuu cagaaugugg ucuacaagcu caacggggaa     2340

gcugaggugu uuuuccgcca guccucuauc aaacaaaccg auagaaucau ucauaaggcc     2400

aaucaggcca ucgacaacaa gaauccgcug aacaacaaaa agcagagcuc cuuuaauuac     2460

gaucugauua aagacaagcg guucacacuc gauaaauuuc aguuccacgu cccuaucacc     2520

cugaacuuca aggcugaagg aaaugaguau cuuaacacaa aggugaauga guaccugaaa     2580

ucuaacagcg acguuaagau uaucggccuc gacagagggg aacgccaucu gauuuaucug     2640

acccuuauca aucaaaaagg agagcuccug aagcagcagu cccugaacgu gaucgccacg     2700

agccaggaac acgagacaga uuacaaaaau cuccugguca acaaggaaaa ugagcgggcg     2760

aacgcuagac aagacuggaa aaccauugag acaaucaagg aacuuaagga gggcuaucug     2820

ucucaggugg uucaccagau ugccaccaug augguggaug aaaaugccau cgucgugaug     2880

gaggaccuca acgcuggcuu uaugcgcggg cggcaaaaag uggaaagaca gguuuaccag     2940

aagcuggaga aaaugcugau ugagaaacuu aauuaucugg ucuucaagaa caaugaugug     3000

aacgaaaccg ccggagugcu caacgcucug cagcucacaa auaaguuuga guccuucgaa     3060

aagaugggca aacaaagcgg guuucuguuc uacguccccg ccuggaacac gucuaagauc     3120

gacccugcga ccggauuugu ggauuuccuu aaacccaagu acgaguccgu ugagaaagcc     3180

aagcuguuuu ucgaaaaguu ugagagcauc aaauucaaug cugacaagaa cuauuuugaa     3240

uuugaguucg auuacaaaaa guucacagaa aaagccgagg gcucucagac caaguggaca     3300

gugugcaccc auagcgacgu ccgcuaucgg uacaaucccc agacgaaggc uuccgaugag     3360

gugaacguua caaaugaacu caaacugauu uucgacaagu uuaaaaucga guauaagaac     3420

gggaaaaauc ugaagaccga acuucugcuc caggacgaua aacaacuguu cagcaagcuc     3480

cugcacuacc uugcccugac ccucaugcug agacagucua aguccggcac agacauugau     3540

uuuauccuga gcccuguggc gaaaaacgga gucuucuaug acucccgcaa ugccaugccg     3600

aaccuuccca aggaugcuga cgccaauggc gcuuuucaua uugcccugaa agggcucugg     3660

ugugugcagc agaucaagaa agccgaugac cugaagaaga ucaaacucgc uauuucuaac     3720

aaggaguggc ugagcuucgu ucaaaaucuu aaauga                               3756


<210>  4630
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4630
augaucgaca acaccaagga gaaaaaggaa ggcagcgugu ucgauggguu uacaagaaaa       60

uaccagcugu ccaagacgcu ccgguucgag cugcgcccca uucuuaauac cccuaaaaug      120

cuggacgaug aacaagucau caagaacgac gagacaagac ggaagaaaua ugaagccguu      180

aagccguggu uugaucagcu ccaccgcgag uucauugagg acgcucugaa aucuuuuaag      240

uucaaaaauc uugccaucua ccaggauacc uuucagacau ggcaaaagga cagaaagagc      300

aaacagaaga aagauacccu ggugaagauu gaagugggac uccgggagga aaucguccgc      360

agauucgagg agguggcgaa caucuggguu cgguccgaac aguauaaacu gcugggcauu      420

aagaaagagg ggcucggaau gcuguuugaa gcuggcgugu uccgccuucu gaaggagaga      480

uuuaagaaug aaaaagacac aaccgucgau gggaacaaua ucuucgacga guggacgcgg      540

uggaccggau acuuuaagaa auucuucgag acacgcaaga acuuuuauaa aucugaugac      600

accagcacag ccauugccua cagagugauc aaucagaacc uccggcgcuu cugcgaaaau      660

auucaaaucu uugagaagau uuccgaaaag aucgaguucu cugaaguuga gaaaucuuuu      720

gacaucuccu gugcuggcau uuucagccug gccuauuaca acgcuugccu ucugcagggc      780

gggaucgaua ccuacaauaa gauuaucgga ggcgaggugg acgaaaaaga uaagaaaauu      840

cccgggauca acgagcucau caauaaguau agacaggaca acucuggaga aaagauuccu      900

uuucugaaac agcuggauaa gcaaauccau uccgccaaag aggcguucau ugaaagcauc      960

gagacgaaca aggagcucgu cggcaaacug aagacauuuu acgaaaaugc cgaggugaag     1020

auucagucuu uccggaaccu uaucgcugac aucgugaccg auuauuccgg guacgacauu     1080

gauaaaaucu aucugacaaa ggaagccguc agccacaaug cuucuagaug guuugccucc     1140

uucgagagcu uugagcgcga ccucuucgcg guuguggccg aaaaacagaa caagcuggug     1200

uacgagcuuc ugcggaccca uaaaaaugau uccaagauuu cugacaaaga cggaaaguuc     1260

agcuuucccg auuucaucaa guguucccac auuaaaagag cucucgaaaa gcaagagggc     1320

cgcaucugga aaggcgaaua uuacgaggac auugucgauu uugagaagau caaagacgug     1380

uucacccagu uucugugcgu uuucaaguuu gaacuggagc agcaguucuu ucggaagaca     1440

acgagcgccc aaaccgggga acagacaaaa aucggauacg agauuuucgu gaccaagauc     1500

aacgaacuca uuacaagaga gaauccuguc aucgaucugg aggagaaaau ugcuaucaag     1560

aacuuugccg acgccacccu ucugaucuau cagauugcua aauacuucgc gguggaaaag     1620

cgccggggcu ggcucgauaa uuaugaccug gaugacagau uuuacaaguc uuccgauauc     1680

ggguaucuua acuucuaccg cgacgccuuc gaacagauug uucggcccua uaaucuguuu     1740

agaaacuacc ucacgaaaaa gccuuacaau acaaacaaau gggugcugag cuucgagaau     1800

ccgacccugg cugauggaug ggacaagaac aaagaaaaga ccaacgccgc cgucauccuc     1860

cgcaaggacg gccgguauua ccuggggauu aucaaagagg auuguaaguc ucuuuuugcu     1920

gacagauaua gcaaagagau guccgaagga aucgagucug gcagcuucca aaagauggcc     1980

uacaaauuuu uccccgaagc guccaagaug auuccuaaau gcagcacaca gaccaagaau     2040

gugaaggagc auuuucggaa aucuuccagc gauuauaacc uguuccacga gaaggacuac     2100

aaaaucucug ucgcuauuac aaagaauauc uaugaacuca acaauguguu uuaccgcaaa     2160

gauaacauug aggaauccuu cguucccaag aaugacuuug agaagaaacu gggcgugaag     2220

aaauuccaga gacaguaccu ggaaaucagc cgggauaaca auggguauaa gcaagcccuu     2280

gcccagugga ucgaguucug uauuagauuu cucaaggcuu acaaauccac gaccaucuuc     2340

gacuauucuc gccugcggga ggccaaggaa uacgagagcc uggaugcuuu uuaucaggac     2400

auuaacgccc ucacauacaa uaucuccuuc gucccuauuu cugaacagua uaucaaagag     2460

aagaacgaua auggagaacu guuucuuuuc gagauuuaca acaaagacug gagccugggc     2520

cccauggaua agaauagaaa acgcaccaag aaccuccaca cacuguacuu ugagcaacug     2580

uucuccaagg aaaacgagca ggaaaauuuu cuuuuccagc ucaacgggga ggcggaacug     2640

uuuuucagac cgaaaaccga ggagaagcgg cugggauaua aaguguggga cgccggcgaa     2700

aagaaauggg uuaaggcuaa ggagaaagaa gacggggccg ugaucgaucg caagagauac     2760

gcuaaagaca ucauucucuu ucauugcccu aucacgcuga aucgggucag cgagucuaag     2820

accaaacgcg agauggaugu ggaaauuaga gaggugcuuu ccagcacacc cggcguucac     2880

aucauuggag ucgaccgggg cgaaaagcau cuggccuauu acucugugau cgaucaaaac     2940

gggaaaauca uugagaccga cacacucaau uccaucggaa aggauggccg cgggaagccu     3000

guggaauaug cgagcaaacu ggagaagaga gcccaggagc gggaagcuag ccgcagagac     3060

ugggaggaag ucgaggccau uaaagaucug aagaaaggau acaucagcca ggugauucgg     3120

aaccuugcug accucaucau caagcacaau gccauuaucg uuuucgaaga ucugaacaug     3180

agauucaagc agauucgcgg cgggaucgag aaauccgccu aucaacagcu ggagcgggcu     3240

cucauugaca agcugagcuu ucuugugaaa aagggcgaag aggaccccaa acagaccgga     3300

cacauccuga gagcguacca gcucgccgcu cccgucauug ccuucaagga uaugggcaag     3360

caaacggggc ugaucuuuua cacacaggcc ggauauaccu cuaaaacaug uccugaaugc     3420

ggcuaccgcc ggaauaucaa gugucuguuc gagaacauug aacaggcuaa aacccuuauc     3480

gagaaucucg aguccauuaa cuauaauaag aaagaagacg uguuucagau cagcuacucc     3540

cuggagaagc ugucuagcaa ggaucagaaa aaggagaaaa agguuuccaa cgaacucuau     3600

gccaaaaccc ugaagaaaga cauuuucauc cuuacaacga agaaugcgcu gagauacaag     3660

ugguaugauc gcuacucuga gaaagcuaag guggccaaac gggggaucga cgaauacaag     3720

ggagagguca acgaaagcga gaccaaaaag ggcgugguua aggaauuuaa ccucacagag     3780

uaucugaaag ggcugcuuaa gaccuacgag auugauuaug aacauggcgg aaucagagag     3840

caaauucugu ccguggcccg cggccgggag uucuacaaag acuuucucua ugcucuguuc     3900

cuccugacag aaaccagaca cucuaucagc gggcggaaua cggauuacau ucagugcccg     3960

gaaugcgagu uugacucccg caagggauuc aaagauauca aggaguuuaa cggcgacgcc     4020

aauggggcgu auaacaucgc uagaaaggga auuaugaucc uugaaaagau uaaacaauuc     4080

aaaaaggaua augacggcaa ccuggagaaa augggguggg gcgaccucag caucucuauu     4140

gaagaguggg auaaguucac acagaaggaa uga                                  4173


<210>  4631
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4631
augaacgccg agaagaaaug cgcuuucaau gaauuuacca accuguacga gcucagcaag       60

acacugaaau ucgaacuuaa gcccgugggc aaaacgaagg agaucauuaa gaauuuuaga      120

gaggaacugg agaaaaacaa uccuauccag cucgacaagg aaauuuccau cgccaaacaa      180

gagauuaaga aagaucugga cauccuucac cgggaauuug uccgccaguc uuuugagcug      240

gcguucaagg agaacaagau caauuaugaa auuuucuucg agaucuacag aaacuuuaaa      300

aaguucagca aaaauaagaa auccgaugaa uauauugcug ccaucaagaa ccucaaagag      360

gaaaagauua agcugguuaa aaagaucgcc gacuacuuug ugaaaaugga ucaggagugg      420

cggaaguauu acccgucuga gaaaaagagc uuccugcuca uguccaaugg ggugauuaac      480

cugcuuaucg acaucaauaa ggaucgcaug gacuauucuu uuauucccaa agaagucugu      540

gagaagauca aaggaaaggg caaaauugaa cuguacgaga aguucaacaa gcauuucggg      600

uaccucagca aauauaauac cgcuagagaa aacuuuuacu ccgaugacgu gaagaacagc      660

gccucuguug cuuaucgggc caucugcgag aaucuggaga aauucuguga uaacaagauu      720

aaaguggaag cggacaauuu uccuaaggag aucaaggaaa aauuccuuga guacuuuaac      780

aagaucacag aucuguccuu cuauaaucgc ugccucagcc agaacggaau ugaagaguac      840

cacaaauuua aggacgagau caauagaucu auuaacgaac uguaucccaa uaacuccaaa      900

aauaagccuc aaaacauccc cuaccugagc aaacucgaca agcagauucu gucugagaag      960

gauaaacagu ccuggaucga agagggcuuc aucgacgaga auaacuuuau uuucgaauuu     1020

gagaaguuca ucaaaaugaa ugauaaguac auuccuagcg aaaagaaacu uuuuaacgag     1080

uucuucaucu uucagaacaa ugacgaaguc cugcaaaaac ucuuccugcu uaaaaagacc     1140

auuaacaauu uugccaacau cuaucuggau gacccgaaaa aucucaugga ucugaugccc     1200

uccaagggga caaagaagcg ggacuaccug ucucuccugg agauuaagga ggcucuuaac     1260

agcuaugccg aaaaucagaa caucggagag acccagaucu ucaaaauuaa guacgaugac     1320

gugaucgaua aaacaaagac cccuuuugaa uguuucauga aaauuuggaa guaugaguac     1380

gaccugcucu auaaugaagg cgacggcaag aaaaacaucg aggaggggug ggaaaagauu     1440

ugcgagaaac ugcagaauaa gaaagaugaa uuuacgggag acuacggcaa guacaaaaag     1500

gagaucaagg auuauuguga aauggcucuu ucccugagcc aacgcaucaa acaucucgac     1560

auuagaauca agaaacggga ugggucugag gacgccgaua acaaucuguu cgagggaguu     1620

gaaaagcugg acgaguuuua cgaucgccuc uucgaaauug uggacgagca caacgcguau     1680

caguaccauc acgaguuuca ggccuuccug accaaaaagc ccuauaagac agauaaauuu     1740

aaacuuuccu ucgacaaucc ucaacugcuc aagggcuggg aucugaacaa ggaacccgac     1800

uacuucgggg cucugcuugu caaaaacgag aaguauuuuc ucgccguguu ccacaagcag     1860

uacgcuaaug aaaucuuuag acagaaaaac aagaauagca ccgugaaaau uaaggaggcc     1920

uacgacgucu ucaacggaaa uuauuacaaa aagguugaac ugaagcagau cccgaaacug     1980

uuuaugaacc ucccucggau cugcuucgcc aagucuaauc aagaggaguu uggccugaca     2040

auugaacuug agaaaaucaa gagcgaguuc gauaaauuuc agaagcugaa gaaagaacgc     2100

caggacaaca aucucaaguu cgaucaggaa aaauuuauua agcugcuggc uuauuacaga     2160

cauugucuug agauccggga ggacuggaaa guguucaagu auaacuucaa accccucuac     2220

aaguauucca aucugggcga auuuuacaga gaucuggaga aggacaccua caaccucgau     2280

cuggugcgcg ucaauucuga auauguggag aaaaagcgga acgaagggaa acuuuaccug     2340

uucgagguuu auaauaagga cuuuagcgau aaauccauug gaagaaagaa cauccacagc     2400

gacuacuuca agucucucuu ucugccugau aaccugacga auaaaaagau cgcgcuugcc     2460

gcugacgccg agauuuucuu ucgcgaaaaa ucccucaaga acaaagauga gaucauuaca     2520

caaaagaaua agaucaaaau ugacaagaac aaucauaaag gcgaaaaggc cuauagcaaa     2580

aaccgguaca ccgacgauaa gaucauucug caucugccca ucaaucucaa cuucggggag     2640

aauaagaacc acaaagacuu uaauuaugag aucaacgauu cucugcuuaa gaauaacaga     2700

gacauuaaaa ucauuggaau cgaucgcggc gaaaaguacc ugcucuacua uuccgugauu     2760

gaccagaaag gggauaucaa ggagauggga gacuucaaug ucgugaacgg cguugauuac     2820

uauagcaaac ugguggcuua cgaagagaag cggaagcagg ccagacugga cuggggcgau     2880

auccagucca uuaaagaacu uaaggagggg uauaucucuc acgucauuca cgagaucugc     2940

gaccucauua aaaaguacaa ugcgaucgug guccuggaag accugagcau gggauuuaaa     3000

cgcggccggu ucuuuagaga gcgccaagug uaucagaagu ucgaacucgc ucuguuccgg     3060

aaacuuaacu acuggguuga uaagaacaag gaggaagggg agaaaggauc cgcccugucu     3120

gccuggcagc ucacacccaa ugagaaggac uuuaaaguga gccagcugga aaaggcuaga     3180

caacugggca uccuuuucua caccgccgcu ggguauaccu ccaagacaug uccugauugc     3240

ggcuuucgga aaaacauuac gcugaaguac gucaaugaca aacagcucaa ggaggaucug     3300

ggaaaacuca agaucaacuu cguggaagac cauuuuaaga uuaaauauaa ucugcuugau     3360

uucgagaaga acaaaaagag cgaaaaagcc aaggacaccg uucugccgaa agaggagcgc     3420

aagaucucuu uuguggccaa uuccaagguc auuagaaucc gguggcacga uauuaaaaca     3480

aagcucgcga aaagagaacu ggacggcgag aagaucauca aaaaguuccc caagggggug     3540

auuaaagaau acgauaucaa cauuugccug aagcgcauca uuggagacaa aaucgauuuu     3600

agcaaggacu cuaucaccga gcagcuuauu aauggccggu ucgggaaaga cuuuuauugg     3660

gagcugaugu acuuccucaa ccugauccuc gaaauuagaa augugguuuc cgagacagaa     3720

aucgauuaua uucaaugucc ugucugcgga uuucauagcg acgauggcuu ccgcgggaac     3780

aaguucaaug gcgacgcuaa cggagccuac aauaucgcua gaaagggccg gcugauccuu     3840

gagaaaauua accagcacaa gaaccugaaa gaacucaaga ccggggaucu gucugugaau     3900

aucguggagu gggacaaagc cgucaacaau ugggauaagu uugugcaggg agacaacacg     3960

gagagcaaga auauugaaau ccccgcgaaa gaggaaauga uuuccguuga ggugcgcgaa     4020

agcuga                                                                4026


<210>  4632
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4632
augaagaaag agaaggaguu caaaucuuuu ggcgacuuca ccaaccugua cgaaaucucc       60

aagacacuca gauuugaacu gaaacccgug gagaauacgc agaccaugcu ugaugaagcc      120

gacgucuucg ggaaggauaa gguuauuaaa gacaaguaua caaaaaccaa gccuuuuauc      180

gauaaacugc accgggaguu cguggacgag ucucucaagg augugagccu guccggacuu      240

aagaaauacu cugaaguccu ggagaacugg aagaaaaaua agaaagacaa ggauauugug      300

aaagaacuca agaaggagga ggaacgccug agaaaagagg uuguggaauu uuucgacaac      360

acagcuaaga aaugggccaa ugagaaguau aaagaacugg gccucaagaa gaaagauauc      420

gggauucugu uugaggagag cgucuucgac cuucugaagg aaaaauacgg agaggaacaa      480

gauuccuuuc ucaaggagga aaaaggcgac uuccugaaga acgagaaggg ggagaaagug      540

ucuaucuuug acgaauggaa gggauucguu ggcuauuuca ccaaauuuca ggagacacgg      600

aagaauuucu acaaaaacga uggcaccgaa acggcgcuug cuacccgcau cauugaccag      660

aaucugaaga gauuuugcga uaacaucgac gauuucaaga aaauuaagaa uaaaaucgac      720

uuuagcgagg uggaaaagaa cuucaauaaa acagccgaug ucuuuucccu cgacuucuau      780

aaccaguguc ugcugcaaaa ggggauugau agcuacaaug aguuuaucgg aggcaaaacc      840

cucgagaacg ggaagaagcu gaaaggagug aacgaacuug ugaaugagua ccggcagaag      900

aacaaaaaug aaaaggucuc uuuccugaaa cuccuggaca agcagauucu uuccgagaag      960

gagaaacuga gcuuuggcau cgaaaacgau gagcagcucc ugguugugcu gaauagcuuc     1020

uaugaaacag ccgaggaaaa gaccaaaauc cuccgcacgc uguuugggga cuucguggag     1080

cauaacgaga auuacgaccu ugauaagaca uauauuucca aagucgcuuu caacaccauc     1140

agccacaagu ggacaaauga aacccauaag uuugaggaac ugcucuacgg agccaugaaa     1200

gaggacaagc cgauuggccu gaacuaugau aaaaaggaag acucuuacaa auuccccgau     1260

uuuaucgcuc uuggcuaucu gaagaagugc cucaauaacc uggacuguga uaccaaauuc     1320

uggaaggaga aauacuacga gaauaacgcc gacaagaaag auaaggacaa aggguuucug     1380

acaggaggcc aaaaugcgug ggaucaguuc cuccagauuu uuaucuucga auuuaaccag     1440

cuguucaacu ccgaggccuu ugacaauaag gggaaggaaa ucaaaauugg auaugauaac     1500

uucagaaagg acuuugagga aaucauuaau caaaaagacu ucaagaacga ugagaaucuu     1560

aaaaucgcua uuaagaacuu cgccgacagc gugcugugga ucuaccagau ggcuaaguau     1620

uuugccaucg agaaaaagcg gggcugggau gacgauuucg aacucuccga guuuuacacg     1680

aauccuucua acggguauag ccuguucuac gaccgcgcgu augaagagau uguucagaaa     1740

uacaaugauc ugagaaacua ccuuaccaag aaacccuaua aggaggacaa guggaaacuc     1800

aauuuugaaa acccuacacu ggccaaugga uucgauaaga acaaagaguc cgacaauagc     1860

accgugaucc ugcggaagaa acgcaaguac uaucucggcc ugaugaagaa aggcaacaac     1920

aagauuuuug aagauagaaa uaaagcugag uucauccgga acauugaauc uggggccuac     1980

gagaagaugg cuuauaaaua ccuucccgac gucgccaaga ugaucccuaa gugcuccaca     2040

caacugaaug aggccaaaaa ccacuuucgc aauagcgcug augaccucga aauuaagaaa     2100

ucuuucagca acccgcugaa gaucaccaaa agaaucuuug accugaauaa cauucaguau     2160

gauaagacga auguguccaa aaagaucucu ggagacaaca agggcauuaa aaucuuccag     2220

aaggaguacu acaaaauuag cggggauuuc gacguuuaua aguccgcgcu uaaugauugg     2280

aucgacuuuu guaaagauuu ccucagcaag uacgacucua caaaggauuu ugacuucucc     2340

auccugcgga aaaccaagga uuauaaaagc cuggacgaau uuuacgugga cgucgccaag     2400

auuaccuaua aaaucucuuu cacacccgug uccgagagcu acauugauca gaagaacaag     2460

aauggagaac ucuaucuguu ugagaucuac aaccaagacu ucgcuaaagg caagaugggg     2520

gccaaaaauc uucauacccu guacuuugag aacgucuucu ccccugaaaa cauuucuaag     2580

aauuuuccca ucaaacucaa cggcaaugcc gagcuguucu uuagaccuaa gagcaucgaa     2640

uccaagaaag agaagcgcaa cuucgugcgg gaaauuguua auaaaaagag auauucugag     2700

gauaaaaucu ucuuucacug ccccauuaca cugaaccgcg agacgggaag caucuaccgg     2760

uucaauaacu augugaauaa cuuucuuucc gaaaauaaca uuaauaucau cggcgucgac     2820

agaggggaga agcaccucgc uuacuauagc gugauugaua aaaacggagu uaagaucggc     2880

gggggaucuu ucaaugaaau uaacaaggug gacuacgcca aaaagcugga ggagcgcgcg     2940

ggcgaacggg agcaguccag aaaagauugg caggucgugg aagggaucaa ggaccugaaa     3000

aagggcuaua uuagccaggu gguucgcgag cucgcugauc uggccaucaa gcauaaugcc     3060

auuaucgucc uugaagaccu gaacaugcgg uuuaaacaaa ucagaggagg cauugagaag     3120

ucuaucuacc agcagcucga gaaagcucug auugauaagc uguccuuccu uguggaaaaa     3180

ggggagaagg acccgaacca ggccggacac auccucaagg cuuaccaacu ggccgcgccu     3240

uuuaccagcu ucaaagauau gggcaagcag acagggauug uguuuuauac ccaggccucu     3300

uacacaagca aaaccugucc caauugcgga uuccgcaaga acaauaacaa auuuuauuuc     3360

gaaaauaaca ucggcaaggc ucaggacgcc cugaagaaac ucaagacguu ugaguacgac     3420

uccgaaaaua aauguuucgg gcugagcuau ugccuuucug auuucgcuaa caaggaggag     3480

gucgagaaaa auaagaacaa aaagcggaau aacgccccuu acuccgacau cgaaaagaaa     3540

gauuguuuug aacugagcac caaggacgcg gugagauauc gguggcauga uaaaaauaca     3600

gagcgcggca agaccuucuu ugaaggagag uccguuuacg aggaaaaaga ggaaaaggag     3660

auuggccaaa caaagagagg gcucgugaaa gaguacgaca ucucuaagug ccugauugga     3720

cuguucgaga aaaccggccu ugauuauaag cagaaccugc ucgacaaaau caauagcggg     3780

aaguuugaug gaacguucua caagaaccug uuuaacuauc ucaaucugcu uuucgaaauu     3840

cggaacucca ucucuggcac agaaaucgac uacauuagcu gccccgagug ucaguuucac     3900

accgauaaau ccaagacaau caaaaauggg gacgauaacg gcucuuauaa uauugccaga     3960

aagggaauga ucauucugga caaaaucaag caauucaaga aagaaaacgg cagccucgac     4020

aagauggggu ggggagagcu guuuauugau cuggaggaau gggacaaauu cgcucagaag     4080

aaaaauaaca auaucaucga uaaguga                                         4107


<210>  4633
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant

<400>  4633
augagcaaga acaaugugug ggacaaauuc accaaccagu acucccuguc uaagacacuc       60

agauuugagc ugaaacccgu cggcaagacg cgggaaaaga ugaaugagca acuuaaguau      120

gauaagcagc ugcagaccuu ccucaaagac caggaaaucg aggaugccua ccaauaucug      180

aagccguuuu ucgacaaacu ucacaaggaa uuuauuacag auagccugga cuccgauacc      240

accaaaaaga ucgacuucuc ugauuaccuc auugacuauc gcgauaagaa agaccugaca      300

aagaaagagg auagccugag aaagaacauc ggggagguuu uugugaaagc uggagaccgg      360

uggaagaaag aaaaguaccc uaaguaugag uggaaaaagg gcuccaaugu ggccaacggg      420

ucuggcauuc uccugacaca ggacguccuu augcugauca aagaucucaa ucccaacgac      480

cagaagauca aaaagaugau ugaagagacc uucaagggau uuuucacgua cuuuagcggg      540

uucaaucuga accgcgagaa uuacuauacc acaaaagugg aaaaggcgac cgcuguugcc      600

acaagaaucg ugcaugauaa ccuuccuaaa uuuugcgaca aucuggucca guucgagugu      660

auuaucaaga aaaagaagaa cggagaagug gaucggauug agcgcaaauc cgaauaccuc      720

aaugccuauc aauaccucaa gagcaaaggc agaaccacgc agaucaagga cgcugagucu      780

ggcaaaauga uugaggccga aguuaucaac gagaacaugu ucuccaucag cuauuuuucu      840

gcuugccugu cccagagcgg gauugaagag uacaaucgga caaucggaca cuauaaccug      900

cugauuaauc uuuacaacca ggccaaggaa ggcgaggaga aggaucugaa aaagucugag      960

aaaaaguuca aacgccuccc caucuuuaag acccuguaca aacaaauugg guguggagaa     1020

aaggaaccuc uuuucuuuuc ccugacacau gacaccaagc uccaggugca ggagaauaaa     1080

gaaaacuaua agaaacccua cagcgucgag caaauccugg agcaggcgaa gcuggccggc     1140

gauaaauauu ucaaggggaa guccaaugac ggaauccuca acaccauucc ugaauuuucu     1200

gauuacaucc ugcagaaaga gagcuauggc ggcguguacu gguccaaggu ggcuauuaau     1260

acaaucagca acagauauuu cgaaaauugg cacacgcuua aagacgcccu gauugacgcu     1320

aaggucuuug agaaagggaa gaagggagau gaggaagacg uuaaaauccc ggagucuauc     1380

gaacuccagg gcuucuuuga ugugcuggac acccaagagg gguggaaguc cgaaggaauu     1440

uucuuuaaaa agagccuuac agaggagcug aaaaacaagg aagccgauac caagaauaaa     1500

aagcggcgcg agaucgugaa agaagcgaag agacccucuc aggcccuccu ggcucuccug     1560

uucgacgaua uucaggagca ugccaaccug uuccuuaauc agagcgacga aauccugaaa     1620

cucgagaagu acuccgagaa agaaucuaag gagaagauua aacaauggau ggauagcgcu     1680

cuggccguca accggauccu gaaguacuuu uucgugaaag aauccaaggc caaaggcgag     1740

cagauugacc cuaucaucag cgaagcucuu aagguucucu uuaacucuga ggaugcgaca     1800

ugguucaagu gguaugacgc ccugcgcaau uaccugacca aaaagcccca ggaugacgug     1860

aaagagaaca agcucaaacu gaauuuugaa aacuccacgc uugcuggggg augggacauu     1920

aauaaggaga gcgauaacua uugcgucauc cugcaggacc aaaagggcaa acagcaccuc     1980

ggcauuaucg ccaagaaaga aaagcagaga ggguacaaua aaauuuucga gcggacaccu     2040

gagaaccccc uguauaagau cgauucugga gaaguguggc agaagaugga guacaaacaa     2100

aucgccguuc cgaccggcau ugggggauuu gugagaaagu guuucaauac cgcucagcag     2160

uauggcugga aaugcccuga caacugucuu aauuccgaag ggaagaucau uaucaaaaac     2220

gaugaggcca aggaaaaucu ggaggcgauu aucgacugcu acaaggacuu uuucacaaaa     2280

uacgagaagg auggcuuuaa auauaagaac uucaauuuua acuucaaaga aagcgaggaa     2340

uacgaggaac ucaacuccuu cuuuucugau gucgagcgcc aaggauauaa gcugggcuuc     2400

aucaccguga auaaaacaau uaucgacaag ugggucgagg augggacgau uuaccuguuu     2460

gaaaucaaga accaggacac caaugauggc aaaaaggacg gacacaaaaa caaucuccau     2520

acaauuuauu ggaaggcucu guucgaggac guggggaaca aacccaaacu uaauggaggc     2580

gccgaacugu uuuaccggaa ggcccuccug aaagauaagc aggagaaaau cgaagacaac     2640

uaugggaagg agaucauuaa aaauuucaga uuuagcaagg agaaguuccu guuucacugu     2700

ccuaucaaac uuaacuacaa ggcuaaaucc uacucucauc ccaaguaugc ccuccccgaa     2760

auuaauaaac agaccaacga guucuuuauc acaauuagcg auaucugcuu ccugggcauu     2820

gaccgcggag aaaagcaccu ggcuuacuau ucccucguua aucaaaagga ggagaucauc     2880

gaucagggca cccugaacau gccuuucgug gacaaagaug ggaagccgcg gagcauuaaa     2940

aaggaaaaau uuagauucga cgccaagguc gauaaauggg ugucugagga aauggacugu     3000

uggaauuaca acgaucuucu ggacgcgaug gccuccaacc gcgacauggc ucggaagaau     3060

uggcagacga ucggaaccau uaaggagcuc aaaaacggcu auaucagcca gguugugaga     3120

aagauugucg aucuggccau cgacaaaaau cgcgggaucc ccucuuuuau ugugcuggaa     3180

aaccuuaaug cuggauucau gcggggcaga caaaagaucg agaaauccgu guaccagaag     3240

cucgagcugg cccuggcgaa gaaacucaac uuuguugucg auaagacagu gcagaauggg     3300

gaaauuggca gcgugaccaa cgcccugcag cuuacaccuc ccgucaccaa uuuccaaaac     3360

aucgagaauc gcaaacaggu gggaauuaug cuguauacgc gggcugacua cacaucucag     3420

accgauccug uuacaggcug gagaaagagc aucuaccuca aaaagggguc cgaagaguau     3480

aucaagagcc aaauuaugga cucuuuugau gacuucggau uugauggcaa aaacuacuau     3540

uucaaguaca ccguggaccu gaucgauccc auuaaaaaga ccuccgagaa aaaguuuaag     3600

aaacagugga cacuguauag cgggaucaau ggaaaguccc uugaccgguu ccgcggcgaa     3660

agaucuagcg auaaaaacgu cuggauuaag acgcugcagg acaucaaaac cacacucgac     3720

gccguguuua uuggguucga ucugcagcac ucccuccggu cucaaaucau caaugacggc     3780

auuaugccga agaaaaucga gaacagccag uucaccggau gggaaucccu gagauuugcu     3840

auugagauga uccagcagau ucgcaauauc ggcguugaaa agggggauga ggacuucauc     3900

cuuucuccug ugcgggauaa gaacgaaaac cauuuugaca gcagaaaagc caaggagaau     3960

gagcccaacu ccggagaugc caauggcgcu uacaacauug cgcgcaaagg gaucauuaug     4020

aaugaacaca ucaagaaaga ccugauuccu uucauccaag auaaggagug ggacgccugg     4080

cucgcuggaa gagaagucug ggauaagugg cugaucgaga acaaagacag ccugauuuau     4140

aagaaauga                                                             4149


<210>  4634
<211>  4203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4634
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc cgacaagaaa       60

uacagcauug gacuggauau cggcaccaac uccguugggu gggcggugau uacagacgag      120

uauaaggugc cgucuaagaa auucaagguc cucggaaaua cggaucggca uagcaucaaa      180

aagaaccuga uuggcgcucu gcuguuugac uccggggaaa ccgccgaggc cacacgccuc      240

aaaagaaccg cucggcgcag auacacacgg cgcaagaaua gaaucugcua ucugcaggaa      300

aucuucucua acgagauggc caagguggau gacagcuuuu uccaccggcu ugaagagucc      360

uuucugguug aggaagauaa aaagcaugag cgccacccca uuuucggaaa uaucguggac      420

gaagucgcuu accaugagaa auauccuacc auuuaccacc ucagaaagaa acugguggau      480

ucuacagaca aggccgaucu gcggcucauc uaucuggcgc uugcccacau gauuaaauuu      540

cgcggccauu uccugaucga gggcgaccuc aaccccgaua auagcgacgu ugacaagcug      600

uuuauucaac uugugcagac cuacaaccag cuguucgaag agaauccuau caacgcuucc      660

ggggucgaug ccaaggcuau ccucagcgcc agacugucua aaucccggcg ccuggaaaau      720

cucauugcgc agcugcccgg agagaagaaa aacggccuuu uugggaaucu gaucgcccuc      780

agccugggac uuacgccuaa cuucaagucu aauuuugacc uggcugaaga ugccaaacuc      840

caacugucca aggacaccua cgaugacgau cuggacaacc uccuggcuca gauuggcgau      900

caguaugccg accuuuuccu ggccgcuaag aaccucagcg augcgauccu gcuuucugac      960

auucugagag ugaauacaga gaucaccaaa gccccgcucu ccgcuagcau gaucaagcgg     1020

uacgacgagc accaucagga ucugacacug cucaaagccc uggugagaca acagcuuccc     1080

gaaaaguaua aagagauuuu cuuugaccag uccaagaacg gguacgccgg auauaucgau     1140

ggcggcgcuu cucaggaaga guucuacaag uuuauuaaac cuauccugga aaagauggac     1200

gggaccgagg agcuccuggu caaacugaau cgcgaagauc uucuccggaa gcaaagaacg     1260

uucgacaacg gaagcauucc ccaccagauc caccugggcg agcugcaugc cauccuccgc     1320

cggcaggaag auuuuuaucc uuuccugaaa gacaauagag agaagauuga aaagauccuu     1380

acauuucgca uucccuacua cguugggccg cuggcgcggg gaaacuccag auucgcuugg     1440

augacccgca aaagcgagga gacaaucacc ccuuggaauu uugaagaggu gguggauaag     1500

ggcgccucug cccaauccuu cauugaacgg augaccaacu uugacaaaaa ucuccccaac     1560

gagaaggucc ugccuaaaca cagccugcuu uaugaguacu ucacagugua uaaugaacuc     1620

acgaagguua aauacgugac cgaggggaug agaaagcccg cuuuucuguc uggagaacag     1680

aagaaagcca ucgucgaucu ucucuucaag acaaaccgca aagugaccgu uaagcagcug     1740

aaagaggacu auuucaagaa gauugaaugu uuugauagcg uggagaucuc cggcgucgag     1800

gaccgguuca augcuucucu uggcacauac caugaccugc ucaaaaucau uaaggauaaa     1860

gacuuucugg auaacgaaga gaaugaagac auccuggagg auauugugcu uacccucacg     1920

cuguucgaag acagagagau gaucgaggaa cggcugaaga cauaugccca ccucuuugau     1980

gacaaaguca ugaagcagcu gaagcgcaga cgguacaccg gguggggaag acuuagccgc     2040

aaacugauua acggcauccg ggauaagcaa uccgggaaaa ccauccucga cuuccuuaag     2100

agcgauggau uugcgaacag aaauuucaug cagcugauuc augacgauuc ucugacauuu     2160

aaagaggaca uccagaaggc ccaggugucc ggccaagggg acagccucca cgaacacauu     2220

gcuaaccugg ccggcucucc cgcuaucaag aaaggcauuc ugcagaccgu uaaggugguc     2280

gaugagcucg ugaaaguuau gggacgccau aagccugaaa auaucgugau cgagauggcc     2340

agagagaacc agacaacgca gaaagggcaa aagaacuccc gggaacgcau gaaaagaauu     2400

gaggaaggaa ucaaggagcu gggcagccag auucuuaagg agcacccggu cgaaaauacc     2460

cagcugcaga augagaaacu cuaccuguau uaccugcaaa acgggcggga cauguaugug     2520

gaucaggaac uugacaucaa ucgccugucc gauuacgacg uggaucauau uguuccccag     2580

ucuuuccuca aggacgauag caucgacaac aaaguccuga caagauccga uaagaaucgg     2640

ggaaaaucug acaacgugcc uagcgaggaa guggucaaga agaugaaaaa uuauuggcgc     2700

caacuccuga acgcgaaacu uaucacccag agaaaauuug acaaucugac aaaggccgag     2760

cggggcgggc ucuccgagcu ggauaaagcu ggcuucauua agcgccagcu gguggaaacc     2820

agacagauca cgaagcacgu ugcccaaauu cuugacagcc ggaugaacac caaauacgau     2880

gagaacgaca agcugaucag agaagugaaa gucauuacac ucaagucuaa acuggugucc     2940

gauuuucgca aggacuucca guucuauaag guucgggaga ucaauaacua ccaccaugcu     3000

cacgaugccu accucaaugc cguggucgga accgcucuga uuaaaaagua ucccaaacuu     3060

gaaagcgagu uuguguacgg cgacuauaag guuuacgaug ugagaaaaau gaucgcgaag     3120

ucugagcagg aaaucgggaa ggccacagcu aaauauuucu uuuacuccaa cauuaugaau     3180

uucuuuaaga ccgagaucac gcuggccaac ggagaaauuc gcaaacggcc ucucaucgag     3240

acaaauggcg aaaccgggga gauugucugg gacaagggaa gagauuucgc cacagugcgc     3300

aaagugcuga gcaugcccca ggucaacauc guuaagaaaa ccgaggugca aaccggcggg     3360

uuuucuaagg aaagcauccu gccgaagaga aauuccgaca aacuuauugc ucggaagaaa     3420

gauugggacc cuaagaaaua uggcggauuc gacagcccca caguggccua cucuguccug     3480

gugguugcga agguggagaa gggcaaaucc aagaaacuca agagcgucaa agaacugcuc     3540

gggaucacga uuauggagcg cuccucuuuu gagaagaacc cuaucgauuu ccuggaagcu     3600

aagggauaca aagaggugaa gaaagaccuu auuaucaagc ugcccaaaua uagccuguuu     3660

gaacucgaga auggccggaa gagaaugcug gccuccgccg gggaacuuca gaagggaaac     3720

gagcuggcuc ucccuucuaa auacguuaau uuccuguauc ucgccagcca uuacgagaag     3780

cugaaaggcu cccccgaaga uaacgagcag aagcagcuuu ucguggaaca acacaaacau     3840

uaucuggacg agaucauuga acagaucucu gaguuuagca agcgggucau ucuggcggau     3900

gcuaaucucg acaaagugcu guccgccuac aacaagcacc gcgauaagcc gaucagagag     3960

caggccgaaa acauuaucca ccuuuucacc cugacaaauc ucggggcucc ugccgcuuuu     4020

aaauauuucg acaccacaau ugaucggaag cgcuacacca gcacgaaaga gguucuggac     4080

gccacacuca uccaucaauc uaucaccggc cuguacgaaa ccagaauuga ucuuucccag     4140

cugggaggcg acaagcggcc cgcggccaca aagaaggcug ggcaggccaa gaaaaagaaa     4200

uga                                                                   4203


<210>  4635
<211>  4986
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4635
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc caacuucaag       60

auucugccga ucgcgauuga ccucggaguu aaaaauacag gcguguuuag cgcuuucuac      120

cagaagggga caucccugga gcggcuugau aacaagaaug gaaaagugua ugaacugucu      180

aaggacagcu acacgcuccu gaugaacaau cgcaccgcca gacggcauca acgcagaggc      240

aucgaucgga aacagcuugu caagcgccug uuuaaacuca uuuggacaga gcagcugaac      300

cuggaauggg acaaggauac ccagcaagcc aucuccuucc ucuuuaauag acggggguuc      360

ucuuuuauca cagacggaua uagccccgag uaccugaaca uugugccuga acagguuaag      420

gcuauccuua uggauauuuu cgacgauuau aauggcgagg acgaucugga cuccuaccuc      480

aaacuggcca ccgagcagga aucuaagauc agcgagauuu auaacaaacu uaugcagaag      540

auccuggaau uuaaacucau gaagcugugc acagauauua aagacgacaa gguguccacc      600

aagacgcuga aagagaucac cagcuacgag uucgaacucc uggcugauua ccuugccaau      660

uauucugagu cccugaagac acaaaaauuu agcuacaccg acaagcaggg caaccucaaa      720

gaacugucuu auuaccacca ugauaaguau aauauccagg aguuccuuaa gcgccacgcg      780

acaauuaacg acagaauccu ggauacccuc cugacggacg aucuggacau uuggaauuuu      840

aacuucgaaa aauucgauuu ugacaagaac gaggagaaac uccagaauca agaagauaag      900

gaccauaucc aggcccaucu gcaccacuuc gucuuugcug ugaacaaaau uaaguccgag      960

auggccagcg ggggacggca ccgcucucag uacuuccaag aaaucacaaa uguucuugac     1020

gagaacaauc aucaggaagg cuaucugaag aacuuuugug agaaucucca caacaaaaag     1080

uacuccaauc ugagcgugaa aaaccuuguc aaucugaucg ggaaccucuc caaucuggag     1140

cugaagcccc ucagaaaaua cuucaacgau aagauucacg cuaaggccga ccauugggau     1200

gaacagaaau uuaccgagac auauugccac uggauccugg gagaauggcg ggugggcgug     1260

aaggaccagg auaaaaagga cggggcgaaa uacucuuaua aggaucuuug uaaugagcug     1320

aaacaaaagg ucaccaaggc cggacucguu gacuuccugc uggaacuuga uccuugccgc     1380

accauucccc cuuaccucga caacaacaau agaaaaccgc ccaaguguca gagccugauc     1440

cugaacccua aauuucucga uaaucaguau cccaacuggc agcaauaccu gcaggagcuu     1500

aagaaacugc aguccauuca gaauuaucuc gacagcuucg agacagaucu gaaggugcug     1560

aagucuucca aagaccaacc uuacuuugug gaauacaaga gcucuaacca gcagaucgcu     1620

agcggccagc gggacuauaa agaucuugac gccagaaucc uccaauucau uuuugaucgc     1680

gucaaggcuu ccgacgagcu gcugcucaau gaaaucuacu uccaggccaa aaagcugaag     1740

cagaaagccu cuagcgagcu ugagaagcug gaauccagca aaaagcucga ugaggugauu     1800

gcuaacucuc aacuguccca gauccugaaa agccagcaua cgaauggcau uuucgaacag     1860

gggaccuuuc uucaccucgu uugcaaguau uacaagcaac ggcagagagc gcgcgacucu     1920

cggcuguaua ucaugcccga guacagauau gauaaaaagc ugcauaaaua caacaauaca     1980

ggacgcuucg acgaugacaa ccagcuccug accuacugua aucacaagcc gcggcagaaa     2040

agauaucaac uucugaacga ucucgccggc gugcugcagg ucuccccuaa uuuucugaag     2100

gacaaaauug ggagcgauga cgaccuuuuc aucuccaagu ggcucgugga acacauccgc     2160

ggauuuaaga aggcuugcga ggauucucug aaaauucaga aagacaaccg gggccugcuc     2220

aaccauaaga ucaauauugc cagaaacaca aaagggaagu gugagaagga aaucuucaau     2280

cugauuugca aaaucgaggg aagcgaagau aagaaaggca acuacaagca cggccuugcc     2340

uaugagcugg ggguucuccu guuuggagaa cccaaugagg cuuccaaacc ugaguucgac     2400

cgcaagauca agaaauuuaa cucuauuuac agcuucgccc agauccaaca gauugcguuu     2460

gcugaacgga agggcaaugc caacaccugu gccgugugcu ccgcugauaa ugcccauaga     2520

augcagcaaa ucaaaauuac ggagcccguc gaagacaaca aggauaaaau cauccugagc     2580

gcuaaggccc agcggcuucc cgcgauuccu acacgcaucg uggacggggc cgucaagaaa     2640

auggcuacca uucuggccaa gaauaucgug gaugacaacu ggcagaauau uaaacagguu     2700

cucucugcua agcaccaacu gcacaucccg aucauuaccg aguccaacgc cuucgaauuu     2760

gagcccgcgc ucgccgaugu gaaaggaaag agccugaagg acagacggaa aaaggcucuu     2820

gagagaaucu cuccugaaaa uauuuucaaa gacaagaaca accgcaucaa agaguucgcc     2880

aagggcauuu ccgcuuauag cggggccaau cugacagaug gcgacuuuga uggagccaaa     2940

gaggaacucg accauaucau uccccggucu cacaagaagu acggcacccu gaacgaugag     3000

gcuaaucuga ucugcgucac aagaggggac aacaaaaaua agggaaaccg caucuucugu     3060

cuuagagauc uggcggacaa uuauaaacuc aagcaguuug aaacgaccga ugaccuggag     3120

auugagaaaa agaucgccga uacaauuugg gacgcuaaca agaaagauuu caaguuuggc     3180

aauuaccgga gcuucaucaa ccucaccccu caggaacaga aagccuuucg ccaugcccug     3240

uuccuugcug acgaaaaucc cauuaagcaa gccgugauca gagcgaucaa caaucggaac     3300

cgcacauuug uuaaugggac ccagagauac uucgcugagg ugcuggccaa caauauuuau     3360

cuccgggcca aaaaggagaa ccugaacacg gacaagaucu ccuuugauua cuucggcauu     3420

ccgaccaucg gaaaugggcg cggcauugcu gaaaucagac agcuguauga gaaagucgac     3480

agcgauaucc aggccuacgc gaagggagac aaaccucagg cuucuuauuc ccaccuuauu     3540

gaugccaugc uggccuucug caucgcugcc gacgaacauc ggaacgaugg cagcauuggg     3600

cucgagaucg acaagaauua cucccuguau ccccucgaua aaaacacagg agaaguguuu     3660

accaaggaca uuuucucuca aaucaagauu acagauaaug aguuuagcga caaaaagcug     3720

gugcgcaaaa aggcuaucga gggcuucaac acccacagac agaugacgcg ggaugggauc     3780

uacgccgaaa auuaccuucc uauucugauc cacaaagagc ugaacgaagu uagaaaggga     3840

uauacaugga aaaauuccga ggaaauuaag aucuuuaagg gcaaaaagua cgacauucag     3900

caacucaaca aucuggucua uugucuuaaa uucguggaca agcccaucuc uaucgauauu     3960

cagaucagca cccuggagga gcuccgcaac auucugacaa ccaauaacau cgcggccacc     4020

gcugaauacu auuacauuaa ucucaaaaca cagaagcugc augaguauua caucgaaaac     4080

uacaacacgg cccuugggua uaagaaauac uccaaggaga uggaguuucu gcggucucug     4140

gcuuauagaa gcgaacgcgu gaaaaucaag uccauugacg augucaaaca agugcucgac     4200

aaggauagca auuucaucau uggcaagauc acccugccuu uuaaaaagga guggcagcgg     4260

cuuuacagag aauggcagaa cacaaccauu aaagacgauu augaguuccu gaagucuuuc     4320

uucaauguua aauccaucac aaagcuccac aagaaagugc gcaaggacuu uagccugccc     4380

auuucuacca acgaaggaaa auuccucguc aagcggaaaa cgugggauaa uaacuucauc     4440

uaccagaucc ugaaugacuc cgauagcaga gccgacggca caaagccguu uauuccugcc     4500

uucgacaucu cuaaaaacga gauuguggag gcuaucauug auuccuuuac cagcaagaau     4560

aucuucuggc uucccaagaa caucgaacug caaaaaguug acaauaagaa cauuuuugcg     4620

aucgauaccu ccaaaugguu cgagguggaa acacccagcg accugcgcga uauugggauc     4680

gccaccauuc aguauaagau cgacaauaac ucucggccua aagucagagu gaagcucgau     4740

uacguuaucg acgaugacag caagauuaau uacuuuauga accauucccu gcuuaaaucu     4800

cgguauccgg auaaggugcu ggagauccuc aaacagagca caauuaucga auuugagucc     4860

ucuggauuua acaagacgau uaaagagaug cugggcauga agcucgcugg gaucuacaau     4920

gaaaccagca acaauaagcg ccccgccgcc accaaaaagg cuggacaggc caaaaagaaa     4980

aaguga                                                                4986


<210>  4636
<211>  3258
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4636
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc caagcggaac       60

uacauucugg gacucgacau cggcauuacc agcguugggu auggaaucau ugauuacgag      120

acacgcgacg ugaucgaugc gggcgugaga cuguucaaag aagcuaaugu cgagaacaau      180

gaagggcggc gcuccaagag aggagcccgg cgccuuaaga gacggcgcag acaucggauc      240

cagcgcguga aaaagcugcu cuuugacuau aaccugcuua cggaucacuc ugagcugagc      300

ggcauuaauc cguacgaagc cagaguuaaa ggccucuccc aaaagcuguc ugaggaggag      360

uucagcgcug cccugcucca ucuggcuaaa cggcgcgggg ugcacaacgu caaugaagug      420

gaagaggaca ccggaaacga gcuuuccaca aaggaacaga ucucuagaaa uagcaaggcc      480

cuggaggaaa aauauguugc ggagcuccag cuggaacggc uuaagaaaga uggcgaggug      540

agagggucca uuaaccgcuu uaagaccagc gacuacguca aagaggccaa gcagcugcuc      600

aaagugcaaa aggcuuauca ucagcuggau cagucuuuca ucgacacaua cauugaucug      660

cucgaaaccc ggagaacaua cuaugaggga cccggcgaag gguccccuuu uggauggaag      720

gacaucaaag agugguacga aaugcugaug ggccacugca ccuauuuccc cgaggagcuu      780

cgcagcguga aguacgccua uaaugcugau cuguacaacg cccucaauga ccugaacaau      840

cuugucauua cgcgggacga aaacgagaaa cuggaauauu acgagaaguu ucagaucauc      900

gaaaacguuu ucaaacaaaa gaagaaaccu acccucaagc agauugcgaa agagauccug      960

gugaaugagg aagauauuaa gggcuacaga gugacaucua ccgggaaacc cgaguuuaca     1020

aaccugaagg ucuaucacga caucaaggau auuaccgccc gcaaagaaau caucgagaau     1080

gcugagcucc uggaccagau ugccaagauc cuuacgauuu accaguccag cgaagauauc     1140

caagaggaac ugacaaaccu caauucugag cugacccagg aagagauuga gcagaucucc     1200

aaccuuaaag gauauacagg cacccauaau cugagccuca aggcuaucaa ccugauucug     1260

gacgaacucu ggcacaccaa ugauaaccaa aucgccauuu ucaaucggcu gaaacuugug     1320

ccuaagaagg uugaccuguc ccagcagaaa gagaucccga caacgcucgu ggaugacuuu     1380

auucugucuc ccgucgugaa gcgcagcuuc auccagucca uuaaaguuau caacgccauc     1440

auuaagaaau acgggcugcc uaaugauauc auuaucgaac uugcucgcga gaagaacagc     1500

aaagacgcgc aaaagaugau uaaugaaaug cagaagcgga acagacagac caacgagcgc     1560

aucgaggaaa ucauucggac aaccggaaaa gagaaugcca aguaucucau cgagaaaauu     1620

aagcugcaug acaugcagga aggcaaaugu cuguacucuc ucgaagcuau cccccuggag     1680

gaucuucuga acaauccuuu uaacuaugag guggaccaca uuauccccag auccgucagc     1740

uucgauaauu cuuucaacaa uaaggugcuc gucaagcaag aagagaacag caaaaagggg     1800

aaucggacac cguuucagua ccuguccucu agcgacucca aaaucagcua cgaaaccuuc     1860

aagaaacaca uucugaaccu ugccaaggga aagggccgca ucucuaaaac gaagaaagag     1920

uaucuccugg aggaaagaga uauuaaucgg uuuuccgugc agaaggacuu caucaacaga     1980

aaucugguug auacacgcua cgccacccgg ggccucauga accugcuuag aagcuauuuu     2040

cgcgugaaua accuggacgu caaagugaag ucuauuaacg ggggauucac cuccuuucuc     2100

agacggaagu ggaaauucaa gaaagagcgc aauaagggcu acaaacauca cgcugaagau     2160

gcccugauca ucgcgaacgc ugacuuuauu uucaaggagu ggaaaaagcu ggauaaggcc     2220

aaaaagguua uggaaaauca gauguuugag gagaaacaag ccgaaagcau gccugagauc     2280

gaaacagagc aggaauauaa ggagauuuuc aucacacccc aucagauuaa acacaucaag     2340

gacuuuaagg auuacaaaua uucccauaga guggacaaga aaccuaaccg ggagcuuauu     2400

aaugacaccc ucuacucuac gcgcaaggau gacaaaggga acacccugau cgucaauaac     2460

cugaauggac ucuacgauaa ggacaacgau aagcugaaga aacuuaucaa uaaaagcccc     2520

gaaaagcugc ucauguauca ccacgacccc cagacauacc aaaaacugaa gcugauuaug     2580

gagcaguaug gcgaugaaaa gaacccucuu uacaaauauu acgaggaaac cgggaauuau     2640

cucacaaagu acuccaaaaa ggacaacggc ccggugauca aaaagauuaa guacuaugga     2700

aauaaacuga acgcucaucu ggauaucacc gacgauuacc ccaacucuag aaauaaggug     2760

guuaaacuca gccugaagcc uuaucgguuc gacgucuacc uugauaacgg cguguauaaa     2820

uucgugacgg ucaagaaucu ggacgugauu aaaaaggaga acuacuauga gguuaauucc     2880

aagugcuacg aagaggccaa aaagcucaaa aagaucagca accaggcuga auuuaucgcc     2940

ucuuucuaca auaacgaccu gauuaaaauc aauggggagc uguaucgcgu gauuggaguc     3000

aacaaugauc uucugaacag aaucgaggug aauaugauug acaucaccua ccgggaauau     3060

cucgagaaca ugaaugauaa gagacccccu agaaucauua agacaaucgc guccaaaacc     3120

caaagcauua agaaauacuc uacagacauc cugggcaacc ucuaugaagu uaaguccaaa     3180

aagcaccccc agauuaucaa gaaagggaag cggccggccg cuaccaaaaa ggccggacag     3240

gcuaaaaaga agaaauga                                                   3258


<210>  4637
<211>  4326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4637
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc ccuguucaac       60

aagugcauua ucauuagcau caaucucgac uuuuccaaca aagagaagug uaugaccaag      120

ccguacucua uuggacugga uaucggcaca aauagcguug ggugggcggu gaucacggac      180

aacuauaaag ugcccuccaa gaaaaugaag guccuuggaa auaccucuaa aaaguacauu      240

aagaaaaacc ugcucggcgu gcugcuuuuc gauagcggga ucacagcuga aggacggcgc      300

cugaagagaa ccgcccggcg cagauauaca cggcgcagaa aucggauucu cuaccugcag      360

gagaucuuuu ccaccgaaau ggccacacug gacgaugcuu ucuuucaacg ccucgacgau      420

ucuuuccugg uuccugacga uaaaagagac agcaaguauc ccauuuuugg caaccuugug      480

gaggaaaaag ucuaccauga ugaguucccu accaucuauc aucugcggaa guaccucgcc      540

gacuccacga aaaaggcuga ucugcgccuu guguaccugg cccucgcgca caugauuaag      600

uauagaggcc acuuucugau cgagggggag uucaauagca aaaacaauga cauccagaag      660

aacuuucagg acuuccugga uaccuacaau gccauuuuug aaucugaccu cucccuggaa      720

aacagcaaac agcuugagga gaucguuaag gauaaaauuu cuaagcugga aaagaaagac      780

cggauccuca agcuguuccc cggagagaaa aauuccggca uuuucagcga auuucuuaag      840

cugaucgugg ggaaccaagc ugauuuccgc aaaugcuuua accucgacga gaaggccucu      900

cugcauuucu ccaaggaaag cuaugaugag gaccuggaga cacuccuggg auacaucggc      960

gaugacuauu ccgaugucuu ucuuaaagcu aagaaacugu acgacgccau ucuccugucu     1020

ggguuccuua ccgugacaga caaugaaacc gaggcgccuc ugagcuccgc caugaucaag     1080

agauauaacg aacacaaaga ggaucucgcu cugcugaagg aauacauucg gaauaucagc     1140

cucaagacgu acaacgaggu guuuaaagac gauacaaaga auggauaugc cggcuacauu     1200

gacggcaaaa ccaaccagga ggauuucuau gucuaccuga agaaucuucu ggcugaauuu     1260

gagggggccg acuauuuccu cgaaaagauc gauagagagg acuuucugcg caagcagcgg     1320

acauucgaua acggaucuau cccguaccag auucaucugc aagaaaugag agccauccuu     1380

gacaaacagg cuaaguuuua ucccuuccuc gcgaagaaua aagagcgcau ugagaagauc     1440

cugaccuucc ggauuccuua cuacguuggc ccccuggcca gagggaacuc cgauuuugcu     1500

uggagcaucc gcaaacggaa ugaaaagauu accccuugga acuucgagga cgugaucgau     1560

aaagaaucua gcgccgaggc cuuuaucaau agaaugacau ccuucgaccu cuaucugccc     1620

gaggaaaagg ugcuuccgaa gcacucucug cucuacgaga cguuuaacgu cuauaaugaa     1680

cugaccaaag ugcgcuucau ugcugagagc augcgggacu accaguuucu ggauuccaag     1740

cagaaaaagg acaucguuag acuuuauuuc aaagauaagc gcaaggugac agacaaagau     1800

auuaucgaau accuccacgc cauuuaugga uacgacggca ucgagcugaa ggggaucgag     1860

aaacaauuua acagcucucu guccaccuac caugaucucc ugaacauuau caaugacaag     1920

gaguuccuug augacagcuc uaacgaagcg auuaucgaag agauuaucca cacacugacc     1980

aucuuugaag aucgggagau gauuaaacag agacucucca aguucgagaa uaucuuugac     2040

aagagcgucc ugaaaaagcu gucccggcgc cacuauacgg gauggggcaa acuuucugcu     2100

aagcucauua acggcaucag agaugaaaag agcgggaaua caauucugga cuaccugauc     2160

gacgauggaa ucuccaaccg gaauuucaug cagcucauuc augacgaugc ccugucuuuc     2220

aagaaaaaga uccaaaaggc ccagauuauc ggcgacgagg auaaagggaa cauuaaggaa     2280

gugguuaaaa gccuuccugg aucccccgcu aucaagaaag gcauucugca gagcaucaag     2340

aucguggacg agcucgucaa ggugaugggc gggagaaaac cugaaucuau ugucguggag     2400

auggcccgcg agaaucagua uaccaaccaa ggaaagucca auagccagca gcggcugaaa     2460

agacuggaaa agucucuuaa agagcucggc uccaagaucc ugaaggaaaa cauucccgcu     2520

aaacugagca agaucgauaa uaacgcccuc cagaaugacc gccuguaccu uuauuaccug     2580

caaaacggga aagauaugua uaccggagac gaucucgaca uugacagacu gucuaacuac     2640

gauaucgacc acaucauucc ccaggcguuu cugaaggaua auagcaucga caacaaaguu     2700

cuugugucca gcgccucuaa ucggggcaag uccgaugacu ucccuagccu ggaggucgug     2760

aagaaacgca agacauuuug guaccagcuc cugaaaucca agcucauuuc ucagagaaaa     2820

uucgauaacc ugaccaaggc ugagcgcggg ggacuucugc cggaagacaa ggccggcuuu     2880

auccaacggc agcucguuga gacaagacag auuacgaaac auguggcucg gcugcuggau     2940

gaaaaguuca auaacaaaaa ggacgagaau aaccgcgccg ucagaaccgu gaaaaucauc     3000

acacuuaaga gcacccuggu gucccaguuu cggaaagauu ucgaacucua uaagguucgc     3060

gagauuaaug acuuucacca ugcgcacgau gccuaccuga acgcugucau cgccucugcu     3120

cuccugaaga aauaucccaa acuugagccu gaguucgugu acggggacua ucccaaauac     3180

aauagcuuua gagaacggaa guccgccaca gaaaaagugu auuucuacuc uaacauuaug     3240

aauaucuuca aaaagagcau uucccuggcc gacggcagag ucaucgagcg cccucucauu     3300

gaagugaacg aggagaccgg agaaagcguu uggaauaaag agucugaucu ggcuacggug     3360

cggagagucc uguccuaccc ccaagugaac guugugaaga aagucgagga acagaaccac     3420

ggccuugacc gcgggaagcc gaaaggacug uuuaaugcga accucagcuc uaagccuaag     3480

cccaauucca acgaaaaucu ggugggcgcc aaagaguauc ucgauccuaa gaaauacggg     3540

ggauaugcug gcaucagcaa cucuuucgcc guucugguga aggggaccau cgagaaaggc     3600

gccaagaaga aaauuacaaa uguccuugaa uuucagggaa ucuccauucu ggaccggauc     3660

aacuacagaa aggauaaacu gaauuuccuc cuggagaagg gcuauaaaga cauugaacuu     3720

aucaucgagc ugcccaagua cagccucuuu gagcuguccg augggagccg ccggaugcuc     3780

gcuucuauuc ugagcaccaa caauaaaaga ggagaaaucc auaagggcaa ccaaauuuuc     3840

cuuucccaga aguuugugaa acugcuguau cacgccaagc gcaucucuaa uacaauuaac     3900

gagaaucauc ggaaauacgu ggaaaaccac aagaaagagu ucgaggaacu cuuuuacuau     3960

auccuggagu ucaaugaaaa cuacgucggg gcgaagaaga acggaaaacu ucugaauagc     4020

gcuuuucagu ccuggcagaa ccacucuauc gacgagcucu guagcuccuu cauuggcccu     4080

accgggucug aaagaaaggg ccuguuugag cucacgagcc ggggauccgc cgccgauuuc     4140

gaguuccugg gcguuaaaau cccccgcuau agagacuaca caccgagcuc ucuucugaag     4200

gaugcuaccc ugauucauca auccgugaca gggcucuaug aaacccggau cgaccuggcc     4260

aaacuuggag agggcaagcg cccugcggcu accaagaaag ccgggcaggc caagaaaaag     4320

aaauga                                                                4326


<210>  4638
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4638
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc cugguacgcg       60

agccugaugu ccgcucauca ccuccggguu ggaauugacg ugggcaccca uucugugggg      120

cuggccacac uucgcgucga ugaccacgga acgccgaucg agcugcucag cgcccugucc      180

cauauucacg auucuggcgu ggggaaggaa gggaaaaagg accacgauac cagaaagaaa      240

cuuagcggca ucgcucggcg cgccagacgg cugcuccauc accgcagaac acagcugcaa      300

cagcuggacg agguucuccg ggaucugggc uuccccauuc cuacccccgg ggaauuucuu      360

gaccugaacg agcagacaga uccuuaucgc guguggagag uccgggcucg ccucguggaa      420

gagaagcugc ccgaggaacu uagaggaccu gccaucucca uggcgguucg gcauaucgcc      480

cgccacagag gcuggcggaa uccguacucu aaaguggaga gccugcucuc ccccgcugaa      540

gagagcccuu ucaugaaggc ccugagagag cgcauucugg cuaccacagg ggaaguccuc      600

gacgauggaa ucacccccgg ccaggccaug gcgcaagugg cccugacgca caacauuucu      660

augcgggggc cugagggaau ccuuggcaaa cugcaucagu ccgacaaugc uaacgaaauu      720

agaaagaucu gcgcccgcca gggcgugagc cccgaugucu guaagcagcu ccugcgggcu      780

guuuuuaaag ccgacucucc gagagggucc gccgugagcc gcguggcucc ugacccccuu      840

ccuggacaag gcucuuuccg gagagcgccc aagugcgauc ccgaguuuca gcgcuuccgg      900

auuaucucca ucgucgccaa ucugagaauu agcgaaacca aaggggagaa ccgcccucuc      960

acagcugacg agcggagaca cgugguuacc uuucugacag aagauuccca ggccgaccug     1020

accugggugg augucgccga gaagcucgga gugcaucggc gcgaccugag aggcacggcu     1080

guucacacag augacgggga acggucugcc gcgagaccgc ccaucgaugc uaccgaccgc     1140

auuaugcggc agacaaaaau cagcucccuu aagaccuggu gggaggaagc cgauagcgag     1200

caaagaggag ccaugauucg cuaucuguac gaggacccua ccgacucuga augugcugag     1260

aucaucgccg aacuccccga ggaagaucag gcuaaacugg acucccuuca ucugccugcc     1320

ggcagagcgg ccuauagccg ggagucucuc acagcucuga gcgaucacau gcuggccacg     1380

accgacgauc uccacgaggc ucgcaagaga cuguucggcg uggacgauuc cugggcgccc     1440

ccggccgaag ccauuaaugc uccugucggg aaccccucug uggaccggac acuuaagauc     1500

gucggacgcu accugagcgc cguggagucc auguggggca ccccugaagu uauucaugug     1560

gagcacguca gagauggguu uacaagcgag cggauggcug acgaacgcga uaaagccaau     1620

agacggcgcu auaacgacaa ucaggaggcc augaagaaaa uccaaagaga uuacggaaag     1680

gaaggcuaca uuucucgggg agacaucgug agacucgacg cucuggagcu gcagggcugc     1740

gcgugucuuu auugcggaac cacgaucggc uaccauacau gucagcucga ucacauuguu     1800

ccccaggccg ggccuggauc caacaaucgc cggggcaacc ugguggcugu cugcgaaaga     1860

uguaaucgca gcaaaucuaa cacccccuuc gccguguggg cccaaaagug cgggaucccg     1920

cacgugggag uuaaggaggc uauuggccgg gucagagggu ggcgcaaaca gaccccuaac     1980

acauccagcg aggaccugac ccggcucaag aaagaaguga ucgccagacu gcgccggaca     2040

caggaggauc ccgaaauuga cgagagaucc auggaaucug uggcguggau ggcuaaugag     2100

cuucaucacc ggaucgccgc cgcuuauccc gagacgaccg ucauggugua ccgcggcagc     2160

auuacagccg cggcuagaaa ggccgccgga aucgauuccc ggaucaaccu gauuggcgaa     2220

aaagggcgca aggacagaau cgaucggaga caucacgcug uugacgccuc uguggucgcu     2280

cucauggagg ccagcguggc gaagacccug gccgaacgcu ccagccugcg gggagagcag     2340

agacuuacag gcaaagaaca aaccuggaag caguauacgg ggucuaccgu uggagcucgc     2400

gagcauuuug agauguggcg gggccacaug cuccaccuga cagaacuguu caaugagaga     2460

cucgccgaag auaaagugua cgucacccag aacauucgcc ugcggcuuuc cgacgggaau     2520

gcucauacag ugaacccuag caagcugguu ucucacagac ucggcgaugg acugaccgug     2580

cagcaaaucg accgcgccug uacgccggcc cuguggugcg cucuuacaag agagaaagau     2640

uuugacgaga agaauggccu ccccgcgcgg gaagaccgcg ccauuagagu ccaugggcac     2700

gagaucaagu ccagcgauua uauccaggug uucucuaaac ggaagaaaac cgacagcgau     2760

cgcgacgaaa caccuuucgg agcuauugcc gugagaggcg gguuugucga gaucggaccc     2820

uccauucauc acgcccggau cuacagaguu gaaggcaaga aaccugugua cgcuaugcug     2880

cgcguguuca cccacgaucu gcucagccag cggcaugggg accuguuuuc ugccgucauu     2940

cccccucagu ccaucagcau gagaugcgcg gagcccaaac uucgcaaagc uaucaccaca     3000

ggcaacgcca cguaucuggg augggugguu gugggcgaug agcucgaaau uaaugucgac     3060

uccuucacca aguacgccau cgggcgguuu cuggaggauu ucccgaacac aaccagaugg     3120

cggauuugug gauaugacac aaauucuaag cugacccuua aaccuaucgu gcucgcugcc     3180

gaaggccugg agaaccccag cuccgcgguu aaugaaauug uggagcugaa gggguggcgc     3240

gucgcuauca acgugcucac gaaaguucac ccuacagugg ucagacggga ugcccuggga     3300

cgccccagau acucuagccg guccaaucuu ccgaccucuu ggaccaucga gaagcgcccu     3360

gccgcuacaa agaaggccgg ccaagcuaag aaaaagaaau ga                        3402


<210>  4639
<211>  4263
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4639
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc caccaagccg       60

uacagcauug gacuggacau cggcacaaac uccguugggu gggcggugac gaccgauaau      120

uauaaagugc ccucuaagaa gaugaaaguc cucggaaaca caagcaagaa auacauuaag      180

aaaaaucugc uuggcgugcu ccuguucgac uccgggauca ccgcugaggg acggcgccug      240

aagagaacag cccggcgcag auauacccgg cgcagaaacc ggauucuuua ccugcaggaa      300

aucuuuucua cagagauggc cacccucgau gacgcuuucu uucaacgccu ggaugacagc      360

uuccugguuc cugaugacaa gagagauucc aaauauccca ucuuuggcaa ucucguggaa      420

gagaaggccu accaugacga guucccuacg auuuaucauc ugcggaaaua ccuugcugau      480

ucuaccaaga aagccgaccu gcgccucguc uaccuggcgc uugcccacau gaucaaguau      540

agaggccacu uucugauuga gggggaguuc aacagcaaaa auaacgauau ccagaagaau      600

uuucaggacu uccucgacac auacaacgcu auuuuugaau ccgaucugag ccuggaaaau      660

ucuaagcagc ucgaagagau cgugaaagac aagauuucca aacuggagaa gaaagaucgg      720

auccuuaagc uguuccccgg agaaaagaac agcggcaucu uuucugaguu ccucaaacug      780

auuguuggga aucaagccga cuuccgcaag ugcuuuaacc uugaugaaaa ggcuucccug      840

cauuucagca aagagucuua ugacgaagau cucgagaccc ugcugggaua caucggcgac      900

gauuauuccg acguguuucu caaagccaag aagcuguacg augcgauucu ucugagcggg      960

uuccucacag ucaccgacaa ugagacggaa gccccucugu ccucugcuau gaucaaaaga     1020

uauaacgagc acaaggaaga ccuugcccug cucaaagagu acauucggaa caucagccug     1080

aagacauaca augaaguguu uaaagaugac accaagaacg gauaugcugg cuacaucgau     1140

ggcaagacaa aucaggagga cuucuaugug uaccugaaaa agcuccuggc cgaguuugaa     1200

ggggccgauu auuuccuuga gaaaauugac agagaagauu uucugcgcaa gcagcggacc     1260

uucgacaacg gauccauccc guaccagauu caccuccaag agaugagagc uauccuggau     1320

aaacaggcga aguuuuaucc cuuccuggcc aaaaauaagg aacgcauuga gaagauccuu     1380

accuuucgga ucccuuacua cgucggcccc cucgcuagag ggaacagcga cuucgccugg     1440

ucuauucgca aacggaauga gaagaucaca ccuuggaacu ucgaagaugu uauugacaaa     1500

gaguccagcg ccgaggcuuu uaucaauaga augacgucuu ucgaucugua ucugcccgaa     1560

gagaaggugc ucccgaaaca uagccugcuu uacgaaaccu uuaacgugua uaaugagcug     1620

acaaaggucc gcuucauugc cgaauccaug cgggacuacc aguuucucga cucuaagcag     1680

aaaaaggaua ucgugagacu guauuucaaa gacaagcgca aaguuaccga uaaggacauu     1740

aucgaguacc ugcacgcgau cuauggauac gauggcauug aacuuaaggg gaucgagaaa     1800

caauuuaaca gcucccucag cacauaccau gaccugcuga auauuaucaa cgauaaggag     1860

uuccucgacg auucuuccaa ugaagcuauu aucgaggaaa ucauucacac ccugacgauc     1920

uuugaggacc gggaaaugau uaaacagaga cuuagcaagu ucgagaacau cuuugauaaa     1980

ucugugcuga agaagcucuc ccggcgccac uauacaggau ggggcaaacu gagcgccaag     2040

cugauuaacg gcaucagaga cgagaaaucc gggaauacca uccuugauua ccucauugac     2100

gacggaaucu cuaaccggaa uuucaugcag cugauucaug augacgcccu gagcuuuaag     2160

aaaaagaucc aaaaagcuca gauuaucggc gaugaagaca aggggaacau caaggagguc     2220

gugaaauccc ucccuggauc ucccgccauu aagaaaggca uccugcagag cauuaagauc     2280

guugaugaac uugugaaagu caugggcggg agaaagccug aguccauugu ggucgaaaug     2340

gcucgcgaga aucaguauac caaccaagga aagagcaauu cucagcagcg gcugaaaaga     2400

cucgagaagu cccugaaaga acugggcagc aagauccuua aagagaacau ucccgccaag     2460

cucucuaaga ucgacaauaa cgcgcugcag aaugaucgcc uguaccucua uuaccugcaa     2520

aacgggaaag acauguauac aggagaugac cuugauaucg acagacuguc caauuacgac     2580

auugaucaca ucauucccca ggccuuccuc aaggacaaca gcaucgauaa uaaagugcug     2640

guuucuagcg cuuccaaccg gggcaagagc gacgaugugc cuucucugga agucgugaaa     2700

aagcgcaaga ccuucuggua ccagcuucug aaauccaagc ucauuagcca gagaaaauuu     2760

gacaaccuga caaaggccga gcgcggggga cucuccccgg aggauaaagc uggcuucauc     2820

caacggcagc ugguugaaac gagacagauc accaagcaug uggcccggcu ucuggacgag     2880

aaguuuaaua acaaaaagga ugaaaauaac cgcgcgguca gaacagugaa aauuaucacc     2940

cucaagucua cacuggugag ccaguuccgg aaagacuuug agcuguauaa gguucgcgaa     3000

auuaaugauu uccaccaugc ccacgacgcu uaccuuaacg ccgucguggc uuccgcccug     3060

cucaaaaagu aucccaagcu ggagccugag uuuguguacg gggauuaucc caaauacaau     3120

ucuuucagag aaagaaagag cgccaccgag aaagucuauu uuuacuccaa caucaugaau     3180

auuuucaaga aaucuaucag ccucgcugac ggccggguga ucgaacgccc ucugauugag     3240

guuaacgaag agacgggaga guccgugugg aauaaggaaa gcgaccuugc gaccguccgg     3300

agagugcugu cuuaccccca aguuaacgug gucaagaaag uggaggaaca gaaucacggc     3360

cucgaucgcg ggaagccgaa aggacuguuu aacgccaauc uguccagcaa gccuaaaccc     3420

aacucuaacg agaaucuugu uggcgcuaag gaauaucugg acccuaagaa auacggggga     3480

uaugccggca ucuccaacag cuucacagug cucgucaagg ggaccauuga gaaaggcgcc     3540

aagaaaaaga ucacaaaugu gcuggaguuc cagggaauuu cuauccucga ucggauuaac     3600

uacagaaagg acaaacugaa uuuucuucug gaaaagggcu auaaagauau cgagcugauc     3660

auugaacucc ccaaguacuc ccuguucgag cuuagcgacg ggucccgccg gaugcuggcu     3720

agcauccucu cuaccaacaa uaaaagagga gagauucaua agggcaacca aaucuuucug     3780

agccagaaau ucgugaagcu ccuguaucac gccaagcgca uuuccaauac gaucaacgaa     3840

aaucaucgga aauacgucga gaaccacaag aaagaauuug aggaacuuuu cuacuauauc     3900

cuggaguuua augagaacua cguuggggcg aagaaaaaug gaaagcugcu caacucugcu     3960

uuccagagcu ggcagaauca cuccauugau gaacuguguu cuagcuuuau cggcccuaca     4020

ggguccgaga gaaagggccu uuucgaacug accucucggg gaagcgccgc cgacuuugag     4080

uuccucggcg ugaaaauucc ccgcuauaga gauuacacac cguccagccu gcucaaggac     4140

gcuacccuga uccaucaauc ugugaccggg cuuuaugaaa cacggauuga ucuggccaaa     4200

cugggagagg gcaagcgccc ugcggcuacg aaaaaggccg ggcaggccaa gaaaaagaaa     4260

uga                                                                   4263


<210>  4640
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4640
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc cgcggcuuuc       60

aagccgaaca gcauuaauua cauccuggga cucgacauug gcaucgccuc cguugggugg      120

gccauggugg agauugauga agaggaaaac cccauccggc ugaucgaccu uggagugcgc      180

gucuuugaga gagcugaagu gccuaaaacc ggcgauucuc uggccauggc ucggcgccuc      240

gccagaagcg uucggcgccu gacaagacgg cgcgcgcaua gacuucugcg gacgcgcaga      300

cuccugaagc gggagggggu gcugcaggcc gcuaauuucg acgagaacgg acucauuaag      360

ucccugccca auaccccuug gcaacuucgc gccgcugccc uggauagaaa acucacaccc      420

cuggaauggu cugcgguccu ucugcaccuc aucaagcauc ggggcuaucu gagccagcgc      480

aaaaacgagg gcgaaaccgc cgacaaggag cugggggcuc uccugaaagg aguggccggc      540

aaugcucacg cccuucagac aggggauuuu agaaccccug ccgagcuggc ucucaacaag      600

uucgaaaagg aguccggaca uauucggaau cagagaucug acuacagcca cacauuuucc      660

cgcaaagauc ugcaagcgga acuuauccuc cuguucgaga agcagaaaga auuuggcaac      720

ccgcacguua gcgggggacu gaaggagggc auugagaccc ugcucaugac gcagcggccc      780

gcccugucug gcgacgcugu gcagaaaaug cuugggcauu gcaccuucga accugccgag      840

cccaaggccg cuaaaaauac auauaccgcc gaaagauuua ucuggcugac aaagcucaac      900

aaucugcgca uucuugagca aggauccgaa cggccucuga ccgauacgga gagagcgaca      960

cucauggacg agcccuaccg caagagcaaa cugaccuaug cucaggcccg gaagcugcuc     1020

ggccuggaag auacagccuu cuuuaaaggg cuuagauacg gaaaggacaa cgcugaggcc     1080

ucuacccuga uggaaaugaa ggcuuaucac gccaucuccc gcgcgcucga gaaggaaggc     1140

cugaaggaca aaaagagccc gcugaaucuu ucuccugagc uccaggauga gaucgggacc     1200

gccuucuccc uguuuaaaac agacgaagau auuacgggac ggcugaagga cagaauccag     1260

cccgagauuc ucgaagcucu gcuuaaacau aucagcuucg auaaguucgu ccaaauuucc     1320

cugaaggccc uccggcgcau cgugccucug auggagcagg gcaaaagaua cgacgaggcu     1380

ugugccgaaa ucuacggcga ucacuauggg aagaaaaaca ccgaggaaaa gauuuaccug     1440

ccucccaucc ccgcggacga gauucggaac ccgguggucc uuagagcccu cucucaggcu     1500

cgcaaaguua ucaauggagu ggugcggaga uauggcagcc ccgcccgcau ucacaucgaa     1560

acagcuagag aggucgggaa guccuuuaag gaucggaaag agaucgaaaa gcgccaagag     1620

gaaaacagaa aagaccggga gaaggccgcc gcuaaauucc gcgaauacuu uccuaauuuc     1680

gugggagagc ccaagagcaa agauauucug aagcugagac ucuaugagca gcagcauggc     1740

aagugccugu acucugggaa agaaaucaac cuuggccggc ugaaugagaa gggauauguu     1800

gaaauugacc acgcgcuccc uuuuucccgc accugggacg auagcuucaa caauaaagug     1860

cugguccugg gcucugagaa ccagaauaag gggaaccaaa cacccuacga auacuuuaau     1920

ggaaaagaca acagcagaga guggcaggag uucaaggccc ggguggaaac cucccgcuuu     1980

ccgagaucua agaaacagcg gauccuucuc cagaaguucg augaggacgg cuuuaaagaa     2040

agaaaucuga acgauacgcg cuauguuaau cgguuccugu gucaauuugu ggcugacaga     2100

augcgccuca cagggaaggg caaaaagcgg gucuucgcca gcaacggaca gauuaccaac     2160

cugcuuagag gguucugggg ccugcgcaag gugcgggccg agaaugauag acaucacgcu     2220

cucgacgccg ucgugguugc gugcuccacc guggcuaugc agcagaaaau cacacgcuuu     2280

guccgguaca aggagaugaa cgccuucgau ggaaaaacca uugacaagga aacaggcgag     2340

gugcugcauc agaaaacgca cuuuccucag cccugggagu ucuuugccca ggaaguuaug     2400

aucagagugu ucgggaagcc ugauggaaag cccgaauuug aggaggcuga cacccuggaa     2460

aaacuucgga cacuccuggc cgagaagcug agcucucgcc cugaagcggu ccacgaguau     2520

gugacacccc ucuucguguc cagagcuccg aaucggaaaa ugagcggcca ggggcauaug     2580

gaaaccguua agucugccaa acgccuggau gagggagucu ccgugcuuag agugccucug     2640

acccaacuca agcugaaaga ccuggagaag auggucaacc gggaaagaga gcccaaacuu     2700

uacgaagccc ugaaagcucg ccucgaggcc cacaaggacg aucccgcuaa agccuuugcg     2760

gaaccuuucu auaaguacga caaagccggc aaucggacgc aacaggugaa ggcuguuaga     2820

guggagcaag uccagaagac cggggugugg guucgcaacc auaauggcau cgccgauaac     2880

gcuacaaugg ugcgggucga cguguuugag aaaggagaua aguauuaccu gguuccgauu     2940

uacagcuggc aaguggccaa aggcauccuc cccgacagag ccgucgugca ggggaaggau     3000

gaagaggacu ggcagcugau ugaugacucc uucaauuuua aauucucucu ucacccuaac     3060

gaucuggugg aagucaucac caagaaggcu cgcauguucg gauauuuugc gagcugucac     3120

cggggcacag ggaauauuaa caucagaauc caugaccucg aucacaaaau uggaaagaau     3180

ggcauccugg aggggauugg cguuaaaacc gcccuguccu uccagaagua ccagaucgac     3240

gagcuuggaa aagaaauucg ccccugcaga cugaagaagc ggccucccgu gcgcaaaaga     3300

ccugcugcca cgaagaaagc cggccaggcu aagaaaaaga aguga                     3345


<210>  4641
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4641
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc caagaaaccg       60

uacaccauug gacuggacau cggcacaaac agcguugggu gggcggugcu cacggaucag      120

uaugaccugg ugaagcggaa gaugaaaauu gcuggagauu ccgagaagaa acaaaucaag      180

aaaaauuucu ggggcguccg ccuuuuugac gaagggcaga ccgccgccga uagacggaug      240

gcucgcacag ccagacggcg cauugagaga cggcgcaaca gaaucucuua ccugcaggga      300

aucuucgcug aagagaugag caagaccgac gccaauuucu ucugccggcu cuccgauucu      360

uuuuaugugg acaacgaaaa gcgcaauagc agacaucccu ucuuugcgac aauugaggag      420

gaaguugagu accacaaaaa cuauccuacc aucuaccauc ugcgggagga acuugucaau      480

uccucugaga aggccgaucu gcgccucguc uaucuggcuc uggcccacau uaucaaauac      540

agaggcaacu uccucauuga aggcgcucug gacacacaga auaccagcgu ggaugggauc      600

uacaagcaau uuauucagac guauaaccag guuuucgccu ccggaaucga ggacggcagc      660

cuuaaaaagc uggaagauaa uaaagacgug gcgaagaucc ucgucgagaa ggugacccgg      720

aaagaaaagc uggagcgcau ucuuaaacug uaccccgggg agaagucugc cggaauguuu      780

gcucaguuca ucucccucau ugugggcagc aaagggaacu uucaaaagcc uuucgaccug      840

aucgaaaagu cugauauuga gugugccaaa gacuccuaug aagaggaucu ggaaagccuc      900

cuggcucuua ucggagacga guacgccgag cuguucgucg ccgcuaagaa ugcguauucu      960

gccguugugc ucuccagcau cauuacagug gcugaaaccg agacaaacgc caaacugucc     1020

gccucuauga ucgaaagauu ugauacccau gaggaagacc uuggcgagcu gaaggcuuuc     1080

auuaaacucc aucugcccaa gcacuacgag gaaaucuuua gcaauacgga gaagcacggc     1140

uaugccgggu acauugaugg aaaaacaaag caggcggacu ucuacaaaua uaugaagaug     1200

acccuggaaa acaucgaggg cgcugauuac uuuaucgcca aaauugagaa ggaaaacuuc     1260

cuccggaagc agagaacauu ugacaauggg gccaucccuc accagcugca ucuugaggaa     1320

cuggaggcua uucuccacca acaggccaaa uauuacccgu uccugaagga aaacuaugau     1380

aaaaucaagu cccuugucac cuuucgcauu cccuacuucg ugggaccucu ggcuaauggc     1440

cagagcgagu uugccuggcu cacccggaaa gcggacgggg agaucagacc cuggaacauu     1500

gaagagaagg uugacuucgg aaaaucugcc guggauuuua ucgaaaagau gacaaauaag     1560

gacacguauc ugccuaaaga gaacguccug cccaagcacu cccucugcua ccaaaaauac     1620

cugguguaua augaacuuac caagguucgc uacaucaacg aucagggcaa aacaagcuau     1680

uucucuggcc aggagaagga gcagauuuuc aaugaccugu uuaagcaaaa acggaaggug     1740

aaaaagaaag aucucgaacu guuccugaga aacaugagcc augucgaguc cccgaccauc     1800

gaagggcuug aggacucuuu uaauagcucc uacagcacau aucacgaucu ccugaaggug     1860

ggaauuaagc aggaaauccu ggacaacccu gucaauaccg agaugcucga gaacauugug     1920

aaaauccuga cgguuuucga agauaagcgc augaucaaag agcagcuuca gcaauuuucu     1980

gacgugcugg auggcgucgu gcucaagaaa cuggaacgga gacauuacac agggugggga     2040

cgccuguccg cuaagcuccu caugggcauu cgggacaagc agagccaccu gaccauccug     2100

gauuaucuca ugaaugacga cgggcugaac agaaaccuua ugcagcugau uaaugauucu     2160

aaccucuccu ucaaaagcau cauugagaag gagcagguua ccacagccga caaagauauc     2220

caauccaucg uggcugaccu ggccggcucu cccgcgauua agaaaggaau ccugcagagc     2280

cuuaagauug ucgaugaacu cguguccgug augggcuacc cuccccagac caucguuguc     2340

gagauggccc gggaaaauca gacaacgggg aaaggaaaga acaauucucg ccccagauac     2400

aagagccugg agaaagcuau uaaggaauuu ggcucccaaa uccugaaaga gcauccuacc     2460

gacaaccagg agcuccggaa uaacagacug uaucuuuacu aucugcagaa ugggaaggau     2520

auguacacag gacaagaccu cgauauccac aaccugagca auuaugacau ugaucacauc     2580

gugccgcagu cuuucauuac cgacaacucc aucgauaauc uggugcuuac aagcucugcc     2640

ggcaaccgcg aaaaagggga cgacgugccc ccucucgaga uugugcggaa gagaaaggug     2700

uuuugggaaa aacuguacca gggcaaucug auguccaagc gcaaauucga uuaucucacc     2760

aaggcugaga gaggaggccu gacggaagcc gacaaagccc gguuuaucca ucgccagcuu     2820

guugagacca gacaaauuac aaagaacguc gcuaacaucc ugcaccagcg guucaauuac     2880

gagaaggaug accaugggaa caccaugaaa caggugcgca ucguuacacu caagagcgcg     2940

cuggugucuc aguuuagaaa acaauuccag cuguacaagg uccgggaugu gaaugacuau     3000

caccacgccc augaugcuua ccuuaacgga guuguggcca auacccugcu caaagucuau     3060

ccccagcugg aaccugaguu cguguacggc gacuaucacc aguuugauug guucaaggcc     3120

aacaaggcua cggccaaaaa gcaauuuuac acaaauauua ugcuuuucuu ugcgcagaaa     3180

gaccgcauca uugaugaaaa cggggagauc cugugggaca agaaauaucu ugacaccgug     3240

aagaagguca ugagcuacag acagaugaau auuguuaaaa agacagaaau ccaaaaagga     3300

gaguucucca aggcuaccau caaacccaag ggcaacagcu cuaaacugau uccgcggaag     3360

accaauuggg auccuaugaa guacgggggc cucgacuccc ccaacauggc cuaugccgug     3420

gugaucgagu acgcuaaagg aaagaauaaa cuggucuuug aaaagaaaau uauccgcgug     3480

acaauuaugg agagaaaggc cuucgaaaag gaugagaaag cguuucugga ggaacagggc     3540

uaucggcagc cuaagguucu ugcuaaacug cccaaguaca cgcucuauga gugugaagag     3600

gggagacgcc ggaugcuggc cagcgccaac gaagcucaga aaggaaauca acaggugcuc     3660

ccuaaccauc uggucacccu ucugcaccau gccgcuaacu gcgagguguc cgacggcaag     3720

ucucucgauu acaucgagag caauagagaa auguucgccg agcugcuggc gcacguuucc     3780

gaauuugcca agcgcuauac acuugcugag gccaaccuga auaaaaucaa ccagcucuuu     3840

gaacagaaua aggaggggga cauuaaagcu aucgcccaau cuuucgugga ucugauggcc     3900

uuuaacgcua ugggagcgcc cgccagcuuc aaguuuuucg agaccacaau ugaacggaaa     3960

agauacaaua accucaagga gcugcuuaau uccaccauca uuuaccaguc uaucacgggc     4020

cuguaugaaa gccgcaagcg gcuggacgau aaaagaccgg cugccacaaa gaaagccggg     4080

caggcuaaga aaaagaaaug a                                               4101


<210>  4642
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4642
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc caagaaaccg       60

uacagcauug gacuggacau cggcaccaac uccguugggu gggcgguggu gacagaugac      120

uauaaggucc ccgcuaagaa aaugaaggug cucggaaaua cggauaaauc ucauauugag      180

aagaaccugc uuggcgcccu ccuguucgac agcgggaaua ccgccgaaga ucggcgccug      240

aaaagaacag cucggcgcag auacacccgg cgcagaaacc ggauccuuua ucugcaggag      300

auuuucuccg aagagauggg aaagguugac gauucuuucu uucaccgccu cgaagacagc      360

uuccugguga cagaggauaa gagaggcgag cggcauccua ucuuuggcaa ucuggaagag      420

gaagucaaau accacgagaa cuuccccacc aucuaucauc uccgccaaua ccuggccgac      480

aauccugaga agguggaucu uagacugguu uaucucgcuc uggcccacau uaucaaauuu      540

cgggggcacu uccuuauuga aggaaaguuu gacacacgca acaaugaugu gcagagacug      600

uuccaggagu uucucgcggu cuacgacaac accuucgaaa auuccucucu gcaggagcaa      660

aacguccagg uggaagagau ccugacggac aaaauuagca aguccgccaa aaaggaucgg      720

gugcucaagc uguuucccaa ugagaaaagc aacggcagau ucgcugaauu ucuuaagcug      780

aucguuggga aucaggccga cuuuaaaaag cauuucgagc ucgaagagaa agcuccucug      840

caguuuucua aggauaccua cgaagaggag cuugaagugc ugcucgccca aauuggagac      900

aacuaugcgg agcuguuccu guccgccaag aaacucuacg auagcauccu gcuuucuggc      960

auccugacag ugaccgacgu cgggacaaag gcuccgcucu ccgccagcau gauucagcgc     1020

uauaacgaac accagaugga ucuggcucag cuuaaacaau uuauccggca gaagcugucu     1080

gacaaauaca augagguguu cuccgauguu agcaaggacg gauaugccgg cuacauugau     1140

ggcaagacca accaggaagc cuuuuauaaa uaccucaagg ggcugcugaa uaaaaucgag     1200

ggauccggcu acuuccucga caagauugag agagaagacu uucugcgcaa acaacggacg     1260

uucgauaacg ggucuauccc ccaucagauc caccuucagg agaugagagc uauuauccgc     1320

cggcaggcgg aauuuuaucc uuuccuggcc gacaaucaag auagaauuga gaagcuccug     1380

acauuucgca uccccuacua ugugggaccu cuggcucggg gcaagagcga cuucgccugg     1440

cuuuccagaa aaagcgccga uaagauuacc cccuggaacu ucgacgagau cgucgauaaa     1500

gaaucuuccg cugaggccuu uaucaaucgc augacaaacu acgaccucua ucugccgaau     1560

cagaaggugc ugccuaaaca cagccuccug uacgaaaagu ucaccguuua uaacgagcuu     1620

accaaaguga aguacaagac agaacagggg aaaacggcgu uuuucgaugc uaauaugaag     1680

caggagauuu uugacggagu cuucaaagug uaccggaagg ucaccaaaga uaagcugaug     1740

gacuuucucg agaaggaguu cgaugaauuu agaaucgugg accugacagg ccuggacaaa     1800

gaaaacaagg uuuucaaugc cucuuauggc accuaccaug aucuuugcaa aauucucgac     1860

aaggauuuuc uggacaacag caaaaaugag aagauccugg aagauauugu gcucacacug     1920

acccuuuucg aggaccggga gaugauccgc aagagacugg aaaacuauuc cgaucuccug     1980

acgaaagagc aagucaagaa acuggaacgg agacacuaca cagggugggg acgccuuucu     2040

gccgagcuca uucauggcau ccggaacaag gaaagcagaa aaaccauccu ggacuaucug     2100

auugaugacg gaaauuccaa ccgcaauuuu augcagcuca ucaacgauga cgcucugagc     2160

uucaaggagg agauugccaa ggcucaggug aucggggaaa ccgauaaucu uaaccagguu     2220

gugucugaca uugccggcuc ccccgcgauc aaaaagggga uccugcaaag ccucaaaauu     2280

gucgacgagc uggugaagau caugggccac cagccugaaa auauuguggu ugagauggcc     2340

agagagaacc aguucacaaa ucagggacgg cgcaacucuc aacagagacu gaaaggccuu     2400

accgauucca ucaaggaauu ugggagccag auucucaaag agcaucccgu cgaaaauucc     2460

caacugcaga acgaccggcu guuccucuac uaucugcaga auggacgcga uauguacaca     2520

ggcgaggaac uugacaucga uuaccugucu caguaugaca ucgaucacau uaucccccag     2580

gcuuuuauua aggacaacag caucgauaau agagugcuca cguccucuaa ggagaaccgg     2640

gggaaaagcg acgaugugcc uuccaaggac gucgugcgca aaaugaaaag cuacuggucu     2700

aaacugcugu ccgccaaacu uauuacccaa agaaaguucg acaaccugac aaaagcugag     2760

cggggaggcc ucaccgauga cgauaaggcc ggguuuauca aacgccagcu gguugaaaca     2820

agacagauca ccaagcacgu ggcgcggauu cucgacgaga gauucaauac ggaaaccgau     2880

gagaacaaua aaaagauccg ccaagucaag auugugacac ugaaaagcaa ccuuguuucu     2940

aauuuucgga aggaguucga acuguauaaa gugagagaga ucaacgacua ccaucacgcc     3000

caugaugcuu aucucaaugc cgucauuggc aaggcucugc ugggagugua cccgcagcuu     3060

gaacccgagu uuguuuaugg cgacuacccu cacuuccacg ggcauaaaga aaacaaggcc     3120

accgccaaga aauuuuucua cuccaauauc augaacuucu uuaagaaaga ugacgugcgc     3180

acagauaaga auggagagau uaucuggaaa aaggacgaac acaucagcaa cauuaagaaa     3240

guccugucuu auccccaggu gaauaucgug aagaaagucg aggagcagac cggcggguuc     3300

agcaaggaau ccauucuccc uaaaggaaac agcgauaagc ugaucccccg gaaaacgaag     3360

aaguuuuacu gggacacaaa gaaguauggc ggguucgacu cuccgauugu ugcuuacucc     3420

auccucguga ucgcggauau ugagaaaggc aagagcaaaa agcugaagac cgugaaagcc     3480

cuugucggag ugacaaucau ggaaaagaug accuuugaga gagacccugu ugcuuuccug     3540

gagcgcaaag gcuaucggaa ugugcaagaa gagaacauua ucaagcuccc caaauacucc     3600

cuguuuaagc uggaaaacgg gagaaagcgc cuucuggccu cugcccggga gcuccagaaa     3660

ggaaaugaaa uuguccugcc uaaccaucuc ggcacccugc uuuaucacgc uaagaauauc     3720

cauaaagugg augagcccaa gcaccuggac uacguugaua aacacaagga cgaguucaag     3780

gaacugcucg auguggucag caacuuuucc aaaaaguaca cacuggccga ggggaaucuu     3840

gaaaagauca aggagcugua ugcgcagaac aauggagaag accucaaaga gcuggcuucu     3900

agcuucauua accuccugac guuuaccgcc aucggcgccc cugcuacauu caaguuuuuc     3960

gauaaaaaua uugacagaaa gcgguacacc uccacaaccg agauccuuaa cgccacgcug     4020

auucaucaau cuaucacagg gcuguaugaa acccgcauug aucucaauaa gcugggcgga     4080

gacaaaagac ccgcggcuac caagaaagcc ggccaggcca agaaaaagaa guga           4134


<210>  4643
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4643
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc ccagacaaca       60

aaccugagcu acauucucgg acuggaccuu ggcaucgcgu ccguugggug ggcuguggug      120

gagauuaaug aaaacgagga uccgaucgga cugauugacg ucggcgugcg gaucuucgaa      180

cgcgccgagg uucccaagac gggggaaucu cucgcccuga gcagacggcu ugcucgcucc      240

accagacggc ugauccgcag acgggcccau cgccuccugc uggcuaaaag auuucucaag      300

cgggagggga uucugucuac aaucgaucuu gagaagggcc ugccuaauca agccugggaa      360

cuccgcgugg cgggccugga gagacggcuu agcgccauug aauggggagc uguccugcuc      420

caccugauca aacaucgcgg auaucugucc aagcggaaaa acgagucuca gaccaauaac      480

aaggagcucg gcgcccugcu uagcggggug gcucagaauc accagcugcu ccaauccgac      540

gauuacagaa cacccgccga acuggcgcuu aaaaaguucg ccaaggagga aggacauauu      600

agaaaccagc gcggcgcuua uacccacaca uuuaaucggc uggaccuccu ggccgagcug      660

aaccuccugu ucgcucagca gcaccaauuu gggaauccuc auugcaaaga acacauccag      720

caguacauga ccgagcuucu gauguggcag aagcccgccc ucagcggaga ggccauucug      780

aaaaugcuug gcaaguguac gcaugaaaag aacgaguuca aagcugcgaa acacaccuau      840

ucugccgaaa gauuuguuug gcugacaaag cucaauaacc ugcgcauccu ggaggauggc      900

gcugaacggg cccucaauga ggaggaaaga caacugcuua ucaaccaccc uuacgagaag      960

uccaaacuga ccuaugccca ggugcgcaag cuccuggggc ugagcgaaca ggcuauuuuc     1020

aaacaucuuc gguacucuaa ggagaacgcc gaauccgcga cauuuaugga gcucaaggcu     1080

uggcacgcca ucagaaaagc ccuggagaau caaggacuga aggacaccug gcaggaucuc     1140

gcuaaaaagc cggaccugcu ugaugaaauu ggcacggccu ucagccugua caaaacagac     1200

gaggauaucc agcaguaucu caccaacaag guccccaauu cugugauuaa cgcucugcug     1260

gugucccuua auuuugacaa auucaucgaa cucagccuga agucccugcg caagauccuc     1320

ccucugaugg agcaagggaa acgguacgac caggccugca gagagauuua uggacaucac     1380

uacggcgaag cgaaccagaa gacaucucag cuucugcccg ccaucccugc ucaagagauu     1440

cgcaaucccg ucguucuccg gacccugagc caggccagaa aagugaucaa cgcuauuauc     1500

cggcaguaug gguccccggc ccgcgugcau aucgaaaccg gaagagagcu gggcaagagc     1560

uuuaaagaac ggagagagau ucagaagcaa caggaggaua aucgcacaaa gcgggaaucu     1620

gcgguccaga aauucaagga gcuuuucucc gacuuuagcu cugaaccuaa aagcaaggau     1680

auccucaaau ucagacugua cgagcagcaa cacggcaagu gucuguauuc cgggaaagaa     1740

auuaacaucc accgccucaa ugagaaggga uacguggaga uugaccaugc ccugcccuuu     1800

ucuagaacgu gggaugacag cuucaacaau aagguucuug ugcuggcuuc cgaaaaccag     1860

aauaaaggca accagacccc uuacgagugg cuccaaggga agaucaacag cgaacggugg     1920

aaaaauuuug ucgcccuggu gcugggaucu cagugcuccg cugccaagaa acagcgccuu     1980

cucacacagg uuauugauga caacaaguuc aucgauagaa aucugaacga cacccgguau     2040

aucgcccgcu uucugagcaa uuacauucaa gagaaccucc ugcuuguggg caagaauaaa     2100

aagaacgucu ucacacccaa ugggcagauc accgcucugc ucagaucucg guggggccug     2160

auuaaagcgc gcgaaaacaa uaacagacac caugcccugg augcuaucgu ggucgccugu     2220

gccacgcccu ccaugcagca gaagauuaca cgguuuaucc gcuucaaaga ggugcacccu     2280

uauaagaucg agaauagaua cgaaaugguu gaccaagaga gcggagaaau uaucuccccg     2340

cacuuucccg agccuugggc uuauuuccgg caggagguga acauuagagu cuuugacaau     2400

caucccgaua ccgugcuuaa ggaaaugcug ccugaccgcc cccaggccaa ccaccaguuc     2460

guucaaccuc ucuucguguc ucgggcgccg accagaaaaa ugagcggcca ggggcauaug     2520

gagacaauca aguccgcuaa acgccuggcc gaaggaauuu cuguccuccg gaucccccug     2580

acccagcuua agccuaaccu gcucgagaau auggugaaca aagaaagaga gcccgcccug     2640

uacgcuggcc ugaaggcccg ccuugcggag uuuaaucaag auccugcuaa ggccuucgcc     2700

acacccuuuu auaaacaagg gggacaacag gugaaggcua uccggguuga acagguccag     2760

aaaagcggcg ugcuggugag agagaacaau ggggucgccg acaacgcuuc cauugugcgc     2820

acggauguuu ucaucaagaa uaacaaauuu uuccucgugc cgauuuacac cuggcagguc     2880

gccaagggca uccugccuaa uaaagcgauu guggcccaca agaacgaaga cgagugggaa     2940

gagauggaug agggagcuaa guuuaaauuc agccucuuuc ccaaugaccu gguugaacuu     3000

aagaccaaaa aggaguacuu cuuuggcuau uacaucgggc uggaucgggc cacaggaaac     3060

aucucucuca aagaacauga cggcgagauu uccaagggga aggauggagu guauagaguc     3120

ggcgugaaac uggcucugag cuucgaaaag uaccagguug acgagcuugg gaaaaaucgg     3180

cagaucugcc gcccccagca gagacagccu gugcggaagc gcccggccgc caccaaaaag     3240

gcuggccaag cgaagaaaaa gaaauga                                         3267


<210>  4644
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4644
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc cgcggcuuuc       60

aagccgaacc ccauuaauua cauccuggga cucgacauug gcaucgccag cguugggugg      120

gccauggugg agauugauga agacgagaac ccuaucugcc ugaucgaucu uggagugcgg      180

gucuuugaac gcgcugaggu gcccaaaacc ggcgacuccc uggccauggc uagacggcuc      240

gcccgcucug uuagacggcu gacacgcaga cgggcgcauc gccuucugag agcccggcgc      300

cuccugaaga gagaaggggu gcugcaggcu gccgauuucg acgagaaugg acucauuaag      360

agccugccua acacgcccug gcaacuucgg gcugccgcgc uggaucgcaa acucaccccu      420

cuggaguggu ccgccguccu ucugcaccuc aucaagcaua gaggcuaucu gucucagcgg      480

aaaaaugaag gcgagacagc ugacaaggaa cugggggccc uccugaaagg aguggcugau      540

aacgcccacg cccuucagac cggcgacuuu agaacaccgg cugagcuggc gcucaauaag      600

uucgagaagg aaagcgggca uauucgcaac cagcggggag auuacuccca caccuuuucu      660

agaaaagacc ugcaagccga gcuuauccuc cuguucgaaa agcagaaaga guuuggcaau      720

ccccacguua gcgggggacu gaaggaaggc auugagacac ugcucaugac ccagcgcccu      780

gcucuguccg gcgaugccgu gcagaaaaug cuugggcauu guacguucga gcccgccgaa      840

ccuaaggcug ccaaaaacac cuauacagcg gagcgguuua ucuggcugac caagcucaau      900

aaccugcgca uucuugaaca aggaagcgag agaccccuga cagacaccga acgggcuacg      960

cucauggacg agccguacag aaagucuaaa cugacauaug cccaggcccg caagcugcuc     1020

ggccuggagg auaccgcuuu cuuuaaaggg cuucgguacg gaaaggacaa ugccgaggcu     1080

uccacacuga uggaaaugaa agccuaucac gcgaucagca gagcccucga aaaggagggc     1140

cugaaggaua aaaagucucc ucugaaccuu ucccccgaac uccaggacga gaucgggacc     1200

gcuuucagcc uguuuaaaac cgaugaggac auuacaggac gccugaagga ucggauccag     1260

ccugaaauuc ucgaggcccu gcuuaaacau aucucuuucg acaaguucgu ccaaauuucc     1320

cugaaggcuc ucagacggau cgugccccug auggaacagg gcaaacgcua cgaugaggcc     1380

ugcgcggaga ucuacggcga ccacuauggg aagaaaaaua cggaagagaa gauuuaccug     1440

cccccuaucc cggccgauga aauuagaaac cccguggucc uucgggcucu cagccaggcc     1500

agaaaaguua ucaacggagu ggugcgccgg uauggcuccc cugcucgcau ucacaucgag     1560

accgccagag aagucgggaa gucuuuuaag gacagaaaag agaucgagaa gcggcaagaa     1620

gagaaucgca aagacagaga aaaggccgcu gcgaaauucc gggaguacuu ucccaacuuc     1680

gugggagaac cuaagagcaa agauauucug aagcugcgcc ucuaugagca gcagcauggc     1740

aagugucugu acuccgggaa agagaucaau cuuggcagac ugaacgaaaa gggauauguu     1800

gagauugacc acgcccuccc cuuuagccgg acaugggaug acucuuucaa uaacaaagug     1860

cugguccugg gcuccgaaaa ucagaacaag gggaaucaaa ccccguacga guacuuuaac     1920

ggaaaagaua auagccgcga auggcaggag uucaaggcua gaguggagac aucucgguuu     1980

ccucgcagca agaaacagag aauccuucuc cagaaguucg acgaagaugg cuuuaaagag     2040

cggaaccuga augacaccag auauguuaac cgcuuccugu gccaauuugu ggccgaucgg     2100

augagacuca cggggaaggg caaaaagcgc gucuucgccu ccaauggaca gauuacaaac     2160

cugcuucggg gguucugggg ccugagaaag gugcgcgcug aaaacgaccg gcaucacgcc     2220

cucgaugcgg ucgugguugc uuguucuacc guggccaugc agcagaaaau caccagauuu     2280

guccgcuaca aggagaugaa ugccuucgac ggaaaaacaa uugauaagga gaccggcgaa     2340

gugcugcauc agaaaacaca cuuuccccag ccuugggagu ucuuugcuca ggaaguuaug     2400

auccgggugu ucgggaagcc cgacggaaag ccugaguuug aagaggccga cacgcccgag     2460

aaacugagaa cccuucucgc ggaaaagcug agcucccggc cggaggcugu ccacgaauau     2520

gugacaccuc uguucgugag ccgcgccccc aacagaaaaa ugucuggcca ggggcauaug     2580

gagaccguua aguccgccaa acggcucgau gaaggaguca gcgugcugcg cgugccccuu     2640

acacaacuga agcucaaaga ccuggagaag auggucaaua gagagcggga accuaagcug     2700

uacgaggcuc uuaaagccag acuggaagcu cacaaggaug acccggccaa agcguuugcc     2760

gagcccuucu auaaguacga uaaagcuggc aaccgcaccc aacaggugaa ggccguucgg     2820

guggaacaag uccagaagac gggggugugg guuagaaauc auaacggcau cgcugacaau     2880

gccaccaugg ugcgcgucga uguguuugag aaaggagaca aguauuaccu cguuccuauu     2940

uacucuuggc aaguggccaa aggcauccug cccgaucggg cugucgugca ggggaaggac     3000

gaggaagauu ggcagcucau ugacgauucc uucaacuuua aauucagccu gcacccuaau     3060

gaccuugugg aggucaucac aaagaaggcg agaauguucg gauauuuugc cuccugccac     3120

cgcggcaccg ggaacauuaa uauccggauc caugaccugg aucacaaaau uggaaagaac     3180

ggcauccucg aagggauugg cguuaaaaca gcucugucuu uccagaagua ccagaucgac     3240

gagcugggaa aagagauuag acccugucgc cuuaagaaga gaccucccgu gcggaaacgc     3300

ccggccgcca ccaagaaagc uggccaggcc aagaaaaaga aguga                     3345


<210>  4645
<211>  3351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4645
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc caaguaccau       60

guuggaauug acgugggcac cuucagcgug gggcuggcgg cuaucgaggu cgaugacgcc      120

ggaaugccga uuaaaacacu cucccuggug ucucacaucc augauagcgg ccuugacccc      180

gaugaaauua aguccgccgu uacgcggcug gcuucuagcg ggaucgcccg cagaacccgg      240

cgccucuaua gacggaagcg cagacggcug cagcaacuug acaaauuuau ccagcgccag      300

ggauggccug ugauugagcu ggaagauuac uccgaccccc ucuauccuug gaaggucaga      360

gcugagcugg ccgcgucuua caucgccgau gaaaaagagc ggggcgagaa gcugagcgug      420

gcucuccgcc acauugccag acaucggggc uggcgcaacc ccuaugcuaa aguuuccagc      480

cuguaccuuc cugacgggcc gucugaugcc uucaaggcga ucagagaaga gauuaagcgg      540

gccuccggac agcccgugcc ugaaacagcu accgucggcc aaauggugac acugugcgag      600

cucgggaccc ugaaacuuag aggagagggc ggggugcuga gcgcccgccu ccagcagucu      660

gacuaugcuc gggaaaucca ggagauuugu agaaugcaag aaaucggaca ggagcuguac      720

cgcaagauca uugaugucgu uuuugccgcc gaauccccca aaggcagcgc uucuucccgg      780

gugggcaagg acccucugca gcccgggaaa aauagagcgc ucaaggccag cgacgcuuuc      840

cagcgcuacc ggaucgccgc ccugauugga aaccuuagag ugcgcgucga uggcgagaaa      900

cggauccugu ccguggagga aaagaaucuc guuuuugacc accuggugaa ccuuacaccg      960

aagaaagagc cugaaugggu caccauugcu gagauccugg ggaucgauag aggacaacuc     1020

auuggcacgg ccaccaugac agacgauggg gaacgcgcgg gagcucggcc cccuacccac     1080

gacacaaaua gaucuaucgu gaacagccgg auugcucccc ugguugauug guggaagacc     1140

gccuccgccc uggagcagca ugccauggug aaagcucuca gcaaugccga ggucgacgau     1200

uucgacucuc ccgaaggcgc gaaggugcag gccuuuuucg cugaucugga cgacgauguc     1260

cacgccaaac uugacucccu gcaucucccu gugggccgcg cugccuauag cgaggauacg     1320

cugguuagac uuacacggag aaugcugucu gacggggugg aucucuacac cgcgcgccug     1380

caagaauuug gaaucgagcc gagcuggaca cccccuacac cccggauugg cgaaccuguc     1440

gggaaccccg ccguggacag aguucugaag accguguccc gcuggcucga gucugcuacc     1500

aagacguggg gagccccgga gagagucauc aucgaacacg ugcgggaggg cuucgugacc     1560

gaaaaacgcg cuagagagau ggauggggac augcggcgca gagccgcccg gaaugcuaag     1620

cuguuucagg agaugcagga aaaacuuaac guucagggca agccuagccg cgcggaucug     1680

uggagauauc aauccgucca gcggcagaau ugccagugug ccuacugcgg aagccccauu     1740

acauucucua acuccgagau ggaccacauc gugccucgcg cuggccaagg gagcaccaau     1800

acaagagaaa accucguggc cgucugucau cggugcaauc agucuaaagg aaacaccccc     1860

uuugccauuu gggcuaagaa cacguccauc gagggcguga gcguuaagga agccguggag     1920

agaacacgcc acugggucac cgauaccggg augcggucca cagacuucaa aaaguucacc     1980

aaagcggugg uugagagauu ucagcgcgcu acaauggaug aagagauuga cgcccggucu     2040

auggaaagcg uggccuggau ggcuaaugag cugagauccc gcgucgccca gcauuucgcg     2100

ucucacggaa cgaccgugcg gguuuauaga ggcagccuga cagcugaagc ccgccgggcc     2160

uccgggauca gcggcaagcu gaaauuuuuc gacggagugg gcaagucuag acucgaucgg     2220

cgccaucacg cuauugacgc cgcugucauc gccuuuaccu ccgauuacgu ggcggagaca     2280

cuggccguga gaagcaaccu gaagcaaucu caggcucacc ggcaggaggc cccucagugg     2340

cgcgaauuua ccgggaaaga cgcugagcau agagccgccu ggcgggucug gugucaaaag     2400

auggaaaaac ucuccgcucu gcuuacggag gaucugagag acgaucgcgu uguggugaug     2460

agcaaugucc ggcucagacu gggaaacggc ucugcgcaca aggaaaccau cgggaaacug     2520

agcaagguga aacuuuccag ccagcucucu guuuccgaca uugauaaggc cagcuccgag     2580

gcucuguggu gcgcccugac acgcgagccc ggauuugacc cgaaggaagg ccucccugcc     2640

aaucccgagc ggcauaucag agugaacggg acccacgucu augcuggcga uaauauugga     2700

cuguuccccg ugucugccgg cagcaucgcg cuucgcgggg gauacgcuga acugggcucc     2760

ucuuuucacc augcccgggu uuacaaaauu acaagcggga agaaaccugc cuucgcuaug     2820

cucagagugu auaccaucga ccugcugccg uaccgcaacc aggaucuuuu cuccgucgag     2880

cucaagcccc aaacgauguc uaugagacag gccgagaaaa agcugcggga cgcgcuggcu     2940

acaggaaaug ccgaauaucu cggcuggcug gugguugacg augagcuugu ggucgacacc     3000

agcaagaucg ccacagauca ggugaaagcu guggaagccg agcuggggac cauucgcaga     3060

uggcgggucg acggcuucuu uuccccuagc aagcuccgcc ugagaccccu gcagaugucu     3120

aaagaaggaa ucaagaaaga guccgcuccu gagcuuagca agauuaucga ucggcccggc     3180

uggcucccug ccguuaacaa gcuguucucu gacgggaaug ugaccguggu cagacgcgau     3240

ucccugggac gggugagacu cgaaagcaca gcgcaccuuc ccguuacgug gaaagugcaa     3300

aagcgcccgg ccgcuaccaa aaaggccggc caggcuaaaa agaagaaaug a              3351


<210>  4646
<211>  3051
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4646
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc cgcgcggauu       60

cuggcuuucg acaucggaau uagcuccauc ggcugggccu uuucugagaa cgaugaacuc      120

aaggacugcg ggguucgcau uuucaccaaa guggagaauc cgaagacagg agaaagccug      180

gcccuuccca gacggcuggc ucgcuccgcc agaaagcggc ucgcucgcag aaaagcccgg      240

cugaaccauc uuaagcaccu gaucgcgaau gaguuuaaac ucaacuacga agauuaucag      300

ucuuucgacg agagccuggc caaggcuuac aaaggcuccc ugaucucucc uuaugagcuc      360

cgcuuuagag cccugaauga acuucugagc aagcaagauu ucgcucgggu gauucuccau      420

aucgccaagc gcagagggua cgacgauauu aaaaacuccg acgauaagga gaaaggagcg      480

auccugaagg ccauuaaaca gaaugaagag aaacuugcua acuaucagag cgucggcgag      540

uaccuguaua aagaauacuu ucagaaguuc aaggaaaauu cuaaagaauu uacgaacgug      600

cggaauaaga aagaguccua cgaacgcugu aucgcccaga gcuuccucaa ggacgagcug      660

aaacugauuu uuaagaagca aagagaguuc ggcuuuucuu ucuccaaaaa guucgaagag      720

gaaguucuca gcguggcuuu uuauaaacgg gcccugaagg auuucucuca ccuugucggg      780

aacugcuccu uuuucaccga cgagaaaaga gcccccaaga auagcccucu ggcuuuuaug      840

uucguggcgc ucacacgcau caucaaccug cuuaauaacc ugaagaacac cgaaggcauu      900

cucuacacaa aagaugaccu gaaugcccug cucaacgagg uucugaagaa uggaacccuu      960

acauauaaac agaccaagaa acugcucggg cuguccgacg auuacgaguu uaagggagaa     1020

aagggcacgu auuucaucga guuuaaaaag uacaaagagu ucauuaaggc ucuuggggag     1080

cauaaccugu cucaggacga ucucaaugaa aucgccaaag acauuacccu gaucaaggau     1140

gagaucaaac ugaagaaggc ccucgcuaaa uaugaccuga accaaaauca gauugauagc     1200

cuuuccaagc uggaguuuaa agaccaccuc aacaucagcu ucaaggcccu gaaacuggug     1260

acaccccuua ugcucgaagg aaagaaguac gaugaggcgu guaaugaacu gaaccugaaa     1320

gucgcuauua augaggacaa gaaagauuuu cucccugccu ucaacgagac cuacuauaag     1380

gacgaaguga caaauccggu gguccugcgg gccaucaaag aguacagaaa gguucuuaac     1440

gcucugcuca agaaauaugg caaggugcac aaaauuaaua ucgaacuggc ccgcgaggug     1500

ggcaagaacc auucucagcg ggcuaaaauc gaaaaggagc agaacgagaa uuacaaggcc     1560

aaaaaggacg cggaacugga gugcgaaaaa cuugggcuca agauuaacuc caaaaauauc     1620

cugaagcuga gacucuucaa agagcaaaag gaauuuugug ccuauagcgg agagaagauu     1680

aaaaucucug aucugcagga cgagaagaug cuugaaauug aucacaucua ccccuauagc     1740

cgcuccuucg acgauucuua caugaacaaa guccuggugu uuaccaagca gaaucaagag     1800

aaacucaacc agacgccuuu cgaggcuuuu ggcaaugaca gcgccaagug gcagaagauu     1860

gaaguucugg cuaaaaaccu gcccacaaag aaacagaagc ggauccuuga uaaaaauuac     1920

aaggacaagg aacaaaagaa cuucaaggau agaaaucuca acgacacccg cuauaucgcc     1980

cggcuggugc ugaauuacac aaaagauuau cucgacuuuc ugccucuuuc cgaugacgag     2040

aacaccaagc ugaaugacac ccagaaaggg agcaaggucc auguggaggc gaagucugga     2100

augcucacau ccgcccugag acacacgugg ggcuucagcg cuaaagaucg caacaaccau     2160

cugcaccacg ccauugacgc uguuaucauu gccuacgcca auaacucuau cgugaaggcu     2220

uuuuccgauu ucaaaaagga acaggagagc aauuccgcgg aacuuuaugc caaaaagauu     2280

ucugagcucg acuacaagaa caaacggaag uuuuucgagc ccuuuagcgg guucagacag     2340

aaaguccugg auaagaucga cgaaaucuuc guguccaaac cggagcggaa gaaaccuucu     2400

ggagcucugc augaagagac cuuucgcaag gaagaggagu ucuaucaaag cuacggcggc     2460

aaggaagggg uccucaaagc ccuggagcuu ggaaagauua gaaaagugaa uggcaagauc     2520

guuaaaaacg gggauauguu ucggguggac auuuucaagc acaagaaaac aaauaaguuu     2580

uacgccgucc ccaucuauac cauggauuuc gcucugaaag ugcucccuaa caaggccguu     2640

gcgagaucca aaaagggaga aauuaaggac uggauccuga uggaugagaa uuacgaauuu     2700

ugcuucagcc uguauaaaga cucucuuauc cugauucaga caaaggauau gcaggagccc     2760

gaguuugugu acuauaacgc uuucaccucc agcacggucu cucucaucgu guccaaacau     2820

gacaauaagu uugaaacacu gagcaaaaac cagaagauuc ucuucaagaa ugccaacgag     2880

aaagaaguga ucgccaaguc uauuggcauc caaaaucuga aaguuuucga gaaguacauc     2940

gucagcgcuc uuggggaagu gaccaaagcc gaguuucgcc agcgggagga cuucaagaaa     3000

aagagacccg cggcuaccaa gaaagccggc caggccaaga aaaagaaaug a              3051


<210>  4647
<211>  3462
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4647
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc cagcgaccug       60

guucucggac uggauauugg caucgggucc gugggagugg gcauucuuaa caaggucacc      120

ggggagauca uucauaaaaa uucucggauc uucccggcgg cucaggccga aaacaaucug      180

gugcgcagaa caaaccggca aggacgcaga cucgcccggc gcaagaagca cagacggguu      240

cgccugaaua gacuuuuuga ggaaagcggc cugaucacgg acuucaccaa aauuuccauc      300

aaccucaauc ccuaccagcu gcgggugaag ggccugacag augagcucuc uaacgaggag      360

cuguuuauug cucuuaaaaa uauggucaag caucgcggga ucagcuaucu ggacgaugcc      420

uccgacgaug gaaacucuag cgugggcgac uacgcucaga uuguuaaaga aaauuccaag      480

cagcucgaaa ccaagacacc ugggcaaauc cagcuggaga gauaucagac cuacggacag      540

cuucggggcg auuucacagu ggaaaaagac gggaagaaac accgccugau uaacgucuuu      600

cccaccagcg ccuauagauc ugaggcgcuc cggauccugc aaacgcagca ggaguucaau      660

ccucagauca ccgaugaauu uauuaacaga uaccuggaga uccucacagg aaagcgcaaa      720

uauuaccaug gccccggcaa ugaaaagucc cggaccgacu acgggagaua ucgcacaagc      780

ggagagaccc uggauaacau uuucggcauc cuuauuggga aaugcacguu uuacccugac      840

gaguuccggg ccgcuaaggc cucuuauaca gcucaagaau uuaaccugcu caaugaccug      900

aacaaucuua ccgugccgac agagaccaag aaacugucca aggaacagaa aaaccagauc      960

aucaauuacg ugaagaacga gaaagccaug ggacccgcga aacucuucaa guauauugcc     1020

aaacugcuga gcugugaugu cgcugacauc aagggcuaca gaauugauaa aucugggaag     1080

gccgaaaucc acaccuuuga ggcuuaucgc aaaaugaaga cacucgaaac gcuggacauu     1140

gagcaaaugg aucgggagac ccuugacaag cuggccuacg uucucacacu gaauaccgaa     1200

agagagggaa uccaggaagc ccuugagcac gaguucgcug auggcuccuu cagccagaaa     1260

cagguggacg aacuggugca auuucgcaag gcgaacuccu cuaucuucgg caaagggugg     1320

cauaauuuua gcgucaagcu caugauggag cugauuccug aacuguacga gacauccgag     1380

gaacagauga ccauccucac gcggcuggga aaacagaaga caaccucuuc uuccaacaag     1440

accaaauaua uugaugagaa acuucugaca gaagagaucu acaaucccgu gguugccaag     1500

agcgugagac aggcuauuaa gaucgucaac gccgccauua aagaauaugg cgacuucgau     1560

aauaucguga ucgagauggc ucgcgaaacc aacgaggacg acgagaagaa agccauucaa     1620

aagauccaga aggcgaauaa agaugaaaag gacgcugcca ugcucaaagc cgcuaaccag     1680

uacaacggga aggccgagcu gccucacucu guuuuucaug gacacaaaca gcuggcuaca     1740

aagauucggc uuuggcacca acagggcgaa agaugccucu auacggggaa gaccaucagc     1800

auucaugauc ugaucaauaa cuccaaucag uucgaggugg accacauccu gccccucucu     1860

auuacauuug augacagccu ggccaacaaa guccuugugu acgcgaccgc caaucaggaa     1920

aagggccaac ggacaccgua ucaggcucug gauuccaugg acgaugccug gagcuuccgc     1980

gagcucaagg cuuuugucag agagucuaag acccugucca acaaaaagaa ggaauaccug     2040

cuuacggagg aagacaucag caaauucgau gugcggaaga aauuuauuga gagaaaucuc     2100

guugacaccc gcuacgccuc ucgggugguc cugaacgcgc ugcaggaaca uuucagagcc     2160

cacaagaucg auacaaaagu guccguugug cgcggacaau uuaccagcca gcucagacgg     2220

cauuggggca uugagaagac acgcgacacc uaucaccacc augcugucga cgcccugauc     2280

aucgcugccu ccucucagcu uaaucugugg aagaaacaga agaacacgcu cgugagcuac     2340

uccgaggauc aacugcugga cauugaaaca ggggagcuua ucucugauga cgaauauaaa     2400

gagagcgugu ucaaggcccc uuaccagcac uucguugaua cccucaaauc caaggaguuu     2460

gaagacagca uucuguucuc uuaucagguc gauuccaaau uuaauagaaa gaucagcgac     2520

gcuacaauuu acgcgacccg gcaggccaag gugggaaaag auaaggcuga cgagaccuau     2580

gugcugggca aaaucaagga uaucuacaca caagacgggu acgaugccuu caugaaaauu     2640

uauaagaagg acaaaucuaa guuucucaug uaccgccaug acccccagac guucgaaaaa     2700

gucaucgagc cuauucugga aaacuauccc aauaagcaga ucaacgagaa aggaaaggag     2760

gugcccugua auccuuuucu uaaguacaaa gaggaacacg gcuauauuag aaaguacucc     2820

aaaaagggga acggcccgga aaucaaaagc cugaaguauu acgauucuaa gcucggaaac     2880

cacauugaca ucacccccaa agauagcaau aacaagguug ugcugcaguc cgucagcccu     2940

uggcgggccg acguguacuu caauaaaaca accggcaagu augagauccu ggggcuuaaa     3000

uacgcugauc uccaauuuga aaagggaaca ggcaccuaua agauuucuca ggagaaauac     3060

aacgacauca agaaaaagga ggggguugau uccgacagcg aguucaaauu uacgcuguau     3120

aagaaugauc ugcuccuggu gaaagacaca gaaaccaagg aacagcaacu uuuccgcuuu     3180

cuguccagaa ccaugcccaa gcagaaacau uacgucgagc ucaagccuua ugauaaacag     3240

aaguucgaag gaggcgaggc ccugauuaaa gugcugggga acguugcgaa uagcggccag     3300

ugcaagaagg gacuuggcaa aagcaacauc uccauuuaca aggugcggac agacguccug     3360

gggaaucaac acaucaucaa aaacgaggga gacaagccca aacucgauuu caagcgcccg     3420

gcugccacca agaaagccgg ccaggcuaag aaaaagaaau ga                        3462


<210>  4648
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4648
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc cacccaguuc       60

gagggauuua caaaccugua ccaaguuagc aagacgcucc gguucgaacu gauuccgcag      120

ggcaaaaccc uuaagcauau ccaggagcag ggguuuauug aagaggacaa ggcgcgcaau      180

gaucacuaua aagaacugaa gcccaucauu gacagaaucu acaaaacaua ugcugaucaa      240

ugccuccagc uggugcagcu ugacugggag aaccuguccg ccgccaucga uucuuaccgg      300

aaggagaaaa ccgaagagac acgcaaugcu cucauugaag aacaggccac cuauagaaac      360

gcuauccaug acuacuucau uggacggaca gauaaucuga ccgacgccau caacaagcgc      420

cacgcggaga uuuauaaggg ccuguuuaaa gccgaacucu ucaaugggaa ggugcugaaa      480

caacuuggaa cggucaccac aaccgagcau gaaaacgcuc ugcucagauc cuuugauaag      540

uucacaaccu acuuuagcgg cuucuacgag aaucggaaaa acguguuuuc ugccgaagac      600

aucagcacgg cuauuccuca ccgcaucguu caggauaauu uccccaaguu uaaagagaac      660

ugucacaucu ucacaagacu gauuaccgcc gugccuuccc uucgggagca uuucgaaaau      720

gucaagaagg cgaucggcau uuuugugucu acaagcaucg aggaaguuuu cuccuuuccc      780

uucuauaacc agcugcucac ccagacccaa auugaccugu acaaucagcu gcucggggga      840

aucagccgcg aggccggcac agagaaaauc aaggggcuga acgaggugcu uaaccuggcu      900

auucagaaaa augaugagac ggcccacauc auugcuucuc ucccucauag auuuaucccg      960

cuguucaagc aaauucuuuc cgaccggaac acccugagcu uuauccucga agaguucaaa     1020

ucugacgaag aggucaucca guccuuuugc aaguauaaga cacugcugcg caaugaaaac     1080

gugcucgaga ccgccgaggc ccuguucaau gaacuuaaca gcauugaucu gacacacauc     1140

uuuauuucuc acaaaaagcu cgagaccauc uccagcgcuc ugugugacca uugggauacg     1200

cuuagaaaug cgcuguacga acggagaauu uccgagcuca caggaaaaau caccaagucu     1260

gccaaagaga aggugcagcg cagccugaag cacgaagaca uuaaccugca ggagaucauc     1320

uccgcugccg gcaaagaacu cagcgaggcc uucaagcaga aaaccucuga aauucugucc     1380

caugcucacg ccgcgcuuga ucaaccccug ccuacaaccc ucaagaaaca ggaggagaag     1440

gaaauccuua agagccagcu ggacucucuu cucgggcugu aucaucugcu cgauugguuu     1500

gcugucgacg agagcaauga aguugauccc gaguucuccg cccggcugac aggaauuaaa     1560

cuugaaaugg agccuucucu gagcuucuac aacaaggcca gaaauuaugc uacgaaaaag     1620

cccuacuccg uggagaaauu uaagcucaac uuccaaaugc cgacccuggc cagcggcugg     1680

gacgugaaua aagaaaagaa caauggcgcu auccuguuug ucaagaacgg gcuuuacuau     1740

cucggaauua ugccuaaaca gaagggccgc uacaaagccc ugucuuucga gcccacagaa     1800

aagaccuccg agggguuuga uaaaauguau uacgacuauu ucccugacgc ggccaagaug     1860

auccccaagu gcagcacaca gcugaaagcu gugaccgccc acuuucagac gcacaccaca     1920

cccauccucc ugucuaacaa uuucauugaa ccucuugaga ucaccaagga gauuuacgau     1980

cugaacaauc cggaaaaaga gcccaagaaa uuucaaacag cuuaugccaa gaagaccgga     2040

gaccagaaag gcuaccggga agcgcucugu aaguggaucg auuucacgag agacuuucug     2100

uccaaauaca caaagaccac aagcauugau cuguccucuc uucgcccuag cucccaguau     2160

aaagaccucg gggaguacua ugccgagcug aacccccugc ucuaccauau cucuuuccag     2220

cggaucgcug aaaaggagau uauggaugcc guugaaaccg gaaagcugua ucuuuuucaa     2280

aucuacaaua aagacuucgc uaagggccac cauggcaaac cuaaccugca cacccucuau     2340

uggacagggc uguucagccc cgagaaucug gccaagacgu ccauuaaacu uaacggacag     2400

gccgaacucu uuuacagacc uaagagccgc augaaacgga uggcucacag acugggcgag     2460

aagaugcuga auaagaaacu caaggaucag aaaaccccga uccccgacac acuguaccaa     2520

gagcuuuaug auuacgugaa ccaucgccug ucucacgacc ucuccgauga agcgcgggcc     2580

cugcugccua augucauuac caaggaggug agccaugaaa ucaucaaaga cagacgguuc     2640

acaucugaca aguuuuucuu ucacguuccc auuacccuua acuaucaggc ugccaauucc     2700

ccuagcaagu ucaaccagcg cgugaaugcc uaccugaaag agcaucccga aacgccgauc     2760

auugggaucg auagaggaga gcggaaccuc auuuauauca cagucauuga cucuaccggc     2820

aagauccugg agcagagaag ccucaacacc auccaacagu uugauuacca gaaaaagcug     2880

gacaaucgcg aaaaagagcg gguggcugcc agacaggcgu gguccgucgu ggggacaauu     2940

aaggaucuua agcaaggcua ucugagccag guuauccacg aaauugugga ccucaugauc     3000

cacuaccagg cugucguggu ucuggagaac cugaauuucg gauuuaaauc uaagcgcacc     3060

ggcauugccg aaaaagccgu guaccagcaa uucgagaaga ugcuuaucga uaaacugaac     3120

ugccucgucc ugaaggacua uccugcugag aagguugggg gagugcucaa ucccuaccag     3180

cugacagauc aguuuacguc cuucgccaaa augggcaccc aaagcggguu ccuguuuuau     3240

gugcccgcgc cuuacacauc caagaucgac ccgcugaccg gauucgucga ucccuuugug     3300

uggaaaacaa uuaagaacca ugaaucuaga aaacacuucc ucgagggcuu ugacuuccug     3360

cauuaugaug ugaagaccgg ggacuuuauc cugcacuuca agaugaaucg gaaccuuagc     3420

uuucagcgcg gccugccugg auucaugccc gcuugggaca uugucuuuga gaaaaaugaa     3480

acgcaguucg augccaaggg caccccuuuu aucgccggga aaagaauugu gcccguuauc     3540

gagaaccauc gguucacagg acgcuacaga gaccucuauc cugcuaauga acugaucgcc     3600

cuccuggagg aaaagggcau uguguuccgg gaugggucca acauccuucc caaacugcug     3660

gagaaugacg auucucacgc uauugacacc auggucgccc ucauccgcag cgugcugcag     3720

augagaaacu ccaaugcggc cacaggagaa gauuacauua acucuccggu ucgggaccuu     3780

aacggcgugu guuuugauag ccgcuuccaa aauccugagu ggcccaugga cgcugaugcc     3840

aacggggcuu accacaucgc ccugaagggc cagcuccugc ucaaucaucu gaaagagucc     3900

aaggaccuua agcugcagaa cggaauuagc aaucaggacu ggcuggccua uauccaagaa     3960

cucagaaaca aacggccugc ugcgaccaag aaagccggcc aggcuaagaa aaagaaguga     4020


<210>  4649
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4649
auggccccca agaaaaagag aaaagugggc auccacgggg ucccugcugc cagcauuuac       60

caggaguucg uuaacaagua uucccugucu aaaacccucc gguuugaacu gaucccgcaa      120

ggaaagacac uugagaauau uaaggcgcgc ggccugaucc ucgacgauga aaagagagcu      180

aaggacuaca aaaaggccaa acagauuauc gauaaguauc aucaguucuu uaucgaggaa      240

auucugagcu ccgugugcau cucugaggac cuucugcaga acuacagcga uguguauuuc      300

aagcucaaaa aguccgacga ugacaaucug caaaaagauu uuaagucugc caaagacacg      360

auuaagaaac agaucagcga guacauuaag gauuccgaaa aguucaaaaa ccuguuuaau      420

cagaaccuca ucgacgcuaa gaaagggcag gagagcgauc ugauucuuug gcugaagcaa      480

ucuaaagaca auggaaucga acucuucaag gccaacuccg acaucaccga uauugacgag      540

gcucuggaga ucauuaagag cuuuaaaggc uggacaaccu auuucaaggg guuucacgaa      600

aaucggaaaa acgucuacuc uuccaaugau auccccacaa gcauuaucua ccgcaucgug      660

gacgauaacc uuccuaaguu ccuggagaau aaagccaagu augaaucucu caaggacaaa      720

gcgcccgagg ccauuaacua cgaacagauc aagaaagauc uggcugagga gcugaccuuu      780

gacauugauu auaagacauc cgaaguuaau cagagagugu ucagccucga cgaggucuuc      840

gaaaucgcca acuuuaacaa uuaccugaac caguccggaa uuaccaaauu caauacgauc      900

aucggcggca aguuugugaa cggggagaau accaagcgga aaggaauuaa cgaauauauc      960

aaucuuuacu cucaacagau uaacgauaag acacugaaaa aguauaaaau gagcguucuc     1020

uucaagcaga uccuguccga caccgagagc aaaucuuuug ugauugacaa acuugaggau     1080

gacuccgaug ucgugacaac caugcagagc uucuacgaac aaaucgcugc cuuuaagacg     1140

guggaggaaa aaucuauuaa ggagacacug agccuccugu ucgacgaucu gaaagcgcag     1200

aagcucgacc uguccaaaau cuacuuuaag aaugauaagu cucuuaccga ccugagccag     1260

caagucuucg augacuauuc cguuaucggc acagccgugc ucgaauacau uacccagcag     1320

aucgcuccua aaaaccugga uaaucccagc aagaaagagc aggagcuuau ugccaagaaa     1380

accgaaaagg cuaaguaucu gucucucgag acaaucaaac uggcccugga agaguuuaac     1440

aagcaucgcg acauugauaa acaauguaga uucgaggaaa uccucgccaa uuuugcugcg     1500

aucccuauga uuuucgacga gaucgcccag aacaaggaca aucuggcuca gauuuccauc     1560

aaauaccaga accaagggaa gaaggaucuu cugcaggcca gcgccgaaga cgaugugaaa     1620

gcuauuaagg accuccugga ucagacgaac aaucugcuuc acaaacucaa gaucuuccau     1680

aucucucagu ccgaggacaa agccaacauu cuggauaagg acgaacacuu uuaucugguc     1740

uucgaggagu gcuacuuuga acucgcgaau aucgugccgc uguauaacaa aauucggaau     1800

uacaucaccc aaaagcccua cagcgaugag aaguucaaac uuaacuuuga aaauuccaca     1860

cuggcuaacg gaugggacaa gaauaaagag ccugauaaca ccgccauucu cuucaucaag     1920

gacgauaaau auuaccuggg cguuaugaau aagaagaaca auaaaaucuu ugacgacaag     1980

gccauuaaag aaaacaaggg ggagggauau aaaaagaucg uguacaagcu gcuucccggc     2040

gcuaauaaaa ugcucccuaa ggucuucuuu ucugccaaaa gcauuaaguu cuauaacccc     2100

uccgaggaua uccugcgcau uagaaaccac ucuacacaua ccaaaaaugg cagcccgcag     2160

aagggguacg aaaaguuuga guucaacauc gaagacuguc ggaaauuuau ugauuucuau     2220

aagcagucca ucagcaaaca cccugagugg aaggacuucg gauuucgcuu cucugauacg     2280

caaagauaca auuccaucga cgaauuuuac cgggaggugg agaaccaggg cuauaaacug     2340

acauucgaaa auauuagcga gucuuacauc gauuccguug ugaaccaggg gaagcucuau     2400

cuguuucaga uuuacaauaa ggacuucagc gcuuauucua aaggacgccc caaccuucau     2460

acccuguacu ggaaggcccu cuuugaugaa agaaaucugc aagacgucgu guauaaacug     2520

aacggcgagg cggagcuuuu cuaccggaag cagagcaucc cuaaaaagau uacccacccc     2580

gccaaagaag cuaucgccaa uaagaacaag gauaauccca aaaaggaguc cgucuuugaa     2640

uacgaccuca ucaaagacaa gagauucaca gaggauaaau uuuucuucca cugcccuauu     2700

accaucaacu uuaagagcuc uggggcuaau aaguucaacg acgaaauuaa ccugcugcuc     2760

aaagagaagg ccaaugaugu gcauauccug uccauugacc gcggagagcg gcaccuugcg     2820

uauuacacac ugguugaugg caaaggcaac aucaucaagc aggacacguu uaauauuauc     2880

gggaacgaua gaaugaaaac caauuaucau gacaagcucg ccgcuauuga aaaggaucgc     2940

gacagcgccc ggaaagauug gaagaaaauc aacaauauua aggagaugaa agaaggauac     3000

cugucccagg ugguccacga gaucgcuaag cuggugauug aauauaacgc caucguugug     3060

uucgaggacc uuaauuuugg cuucaagaga gggcgcuuua aagucgagaa gcaaguguac     3120

cagaaacucg aaaagaugcu gaucgagaaa cugaacuauc ucguguucaa ggauaaugaa     3180

uuugacaaaa caggaggcgu ucugcgggcc uaccagcuua ccgcuccguu cgagacauuu     3240

aagaagaugg ggaaacagac cggcauuauc uacuaugucc ccgcgggauu cacgucuaag     3300

auuuguccug ugaccggcuu ugugaaccaa cuguacccca aauaugaaag cgucuccaag     3360

ucucaggagu ucuucagcaa auuugacaag aucugcuaca aucucgauaa gggguauuuc     3420

gaguuuuccu ucgacuacaa aaacuuugga gauaaggccg cuaaaggcaa guggacaauu     3480

gccucuuucg ggagcagacu gaucaauuuu cgcaacuccg acaaaaauca uaacugggau     3540

acccgggaag uguauccuac aaaggagcug gaaaaacuuc ugaaagacua cagcaucgag     3600

uacggacacg gcgaguguau uaaggccgcu aucugcgggg aaucugauaa aaaguucuuu     3660

gccaaacuca ccuccguucu gaacacgauu cuccagauga gaaauagcaa gacaggcacc     3720

gagcuggacu aucuuaucuc ucccguggcg gaugucaacg gaaauuucuu ugacucccgc     3780

caagcuccga agaacaugcc ucaggaugcc gacgccaaug gcgcuuacca cauugggcug     3840

aaaggacuca ugcugcuggg ccggaucaag aacaaucagg aagggaaaaa gcugaaccuu     3900

gugaucaaaa augaggaaua uuucgaguuu guucagaaca gaaauaacaa gcggcccgcc     3960

gcgacaaaga aggcuggaca agccaagaaa aagaaauga                            3999


<210>  4650
<211>  4716
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4650
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccga caagaaauac agcauuggac uggauaucgg caccaacucc guuggguggg      180

cggugauuac agacgaguau aaggugccgu cuaagaaauu caagguccuc ggaaauacgg      240

aucggcauag caucaaaaag aaccugauug gcgcucugcu guuugacucc ggggaaaccg      300

ccgaggccac acgccucaaa agaaccgcuc ggcgcagaua cacacggcgc aagaauagaa      360

ucugcuaucu gcaggaaauc uucucuaacg agauggccaa gguggaugac agcuuuuucc      420

accggcuuga agaguccuuu cugguugagg aagauaaaaa gcaugagcgc caccccauuu      480

ucggaaauau cguggacgaa gucgcuuacc augagaaaua uccuaccauu uaccaccuca      540

gaaagaaacu gguggauucu acagacaagg ccgaucugcg gcucaucuau cuggcgcuug      600

cccacaugau uaaauuucgc ggccauuucc ugaucgaggg cgaccucaac cccgauaaua      660

gcgacguuga caagcuguuu auucaacuug ugcagaccua caaccagcug uucgaagaga      720

auccuaucaa cgcuuccggg gucgaugcca aggcuauccu cagcgccaga cugucuaaau      780

cccggcgccu ggaaaaucuc auugcgcagc ugcccggaga gaagaaaaac ggccuuuuug      840

ggaaucugau cgcccucagc cugggacuua cgccuaacuu caagucuaau uuugaccugg      900

cugaagaugc caaacuccaa cuguccaagg acaccuacga ugacgaucug gacaaccucc      960

uggcucagau uggcgaucag uaugccgacc uuuuccuggc cgcuaagaac cucagcgaug     1020

cgauccugcu uucugacauu cugagaguga auacagagau caccaaagcc ccgcucuccg     1080

cuagcaugau caagcgguac gacgagcacc aucaggaucu gacacugcuc aaagcccugg     1140

ugagacaaca gcuucccgaa aaguauaaag agauuuucuu ugaccagucc aagaacgggu     1200

acgccggaua uaucgauggc ggcgcuucuc aggaagaguu cuacaaguuu auuaaaccua     1260

uccuggaaaa gauggacggg accgaggagc uccuggucaa acugaaucgc gaagaucuuc     1320

uccggaagca aagaacguuc gacaacggaa gcauucccca ccagauccac cugggcgagc     1380

ugcaugccau ccuccgccgg caggaagauu uuuauccuuu ccugaaagac aauagagaga     1440

agauugaaaa gauccuuaca uuucgcauuc ccuacuacgu ugggccgcug gcgcggggaa     1500

acuccagauu cgcuuggaug acccgcaaaa gcgaggagac aaucaccccu uggaauuuug     1560

aagagguggu ggauaagggc gccucugccc aauccuucau ugaacggaug accaacuuug     1620

acaaaaaucu ccccaacgag aagguccugc cuaaacacag ccugcuuuau gaguacuuca     1680

caguguauaa ugaacucacg aagguuaaau acgugaccga ggggaugaga aagcccgcuu     1740

uucugucugg agaacagaag aaagccaucg ucgaucuucu cuucaagaca aaccgcaaag     1800

ugaccguuaa gcagcugaaa gaggacuauu ucaagaagau ugaauguuuu gauagcgugg     1860

agaucuccgg cgucgaggac cgguucaaug cuucucuugg cacauaccau gaccugcuca     1920

aaaucauuaa ggauaaagac uuucuggaua acgaagagaa ugaagacauc cuggaggaua     1980

uugugcuuac ccucacgcug uucgaagaca gagagaugau cgaggaacgg cugaagacau     2040

augcccaccu cuuugaugac aaagucauga agcagcugaa gcgcagacgg uacaccgggu     2100

ggggaagacu uagccgcaaa cugauuaacg gcauccggga uaagcaaucc gggaaaacca     2160

uccucgacuu ccuuaagagc gauggauuug cgaacagaaa uuucaugcag cugauucaug     2220

acgauucucu gacauuuaaa gaggacaucc agaaggccca gguguccggc caaggggaca     2280

gccuccacga acacauugcu aaccuggccg gcucucccgc uaucaagaaa ggcauucugc     2340

agaccguuaa gguggucgau gagcucguga aaguuauggg acgccauaag ccugaaaaua     2400

ucgugaucga gauggccaga gagaaccaga caacgcagaa agggcaaaag aacucccggg     2460

aacgcaugaa aagaauugag gaaggaauca aggagcuggg cagccagauu cuuaaggagc     2520

acccggucga aaauacccag cugcagaaug agaaacucua ccuguauuac cugcaaaacg     2580

ggcgggacau guauguggau caggaacuug acaucaaucg ccuguccgau uacgacgugg     2640

aucauauugu uccccagucu uuccucaagg acgauagcau cgacaacaaa guccugacaa     2700

gauccgauaa gaaucgggga aaaucugaca acgugccuag cgaggaagug gucaagaaga     2760

ugaaaaauua uuggcgccaa cuccugaacg cgaaacuuau cacccagaga aaauuugaca     2820

aucugacaaa ggccgagcgg ggcgggcucu ccgagcugga uaaagcuggc uucauuaagc     2880

gccagcuggu ggaaaccaga cagaucacga agcacguugc ccaaauucuu gacagccgga     2940

ugaacaccaa auacgaugag aacgacaagc ugaucagaga agugaaaguc auuacacuca     3000

agucuaaacu gguguccgau uuucgcaagg acuuccaguu cuauaagguu cgggagauca     3060

auaacuacca ccaugcucac gaugccuacc ucaaugccgu ggucggaacc gcucugauua     3120

aaaaguaucc caaacuugaa agcgaguuug uguacggcga cuauaagguu uacgauguga     3180

gaaaaaugau cgcgaagucu gagcaggaaa ucgggaaggc cacagcuaaa uauuucuuuu     3240

acuccaacau uaugaauuuc uuuaagaccg agaucacgcu ggccaacgga gaaauucgca     3300

aacggccucu caucgagaca aauggcgaaa ccggggagau ugucugggac aagggaagag     3360

auuucgccac agugcgcaaa gugcugagca ugccccaggu caacaucguu aagaaaaccg     3420

aggugcaaac cggcggguuu ucuaaggaaa gcauccugcc gaagagaaau uccgacaaac     3480

uuauugcucg gaagaaagau ugggacccua agaaauaugg cggauucgac agccccacag     3540

uggccuacuc uguccuggug guugcgaagg uggagaaggg caaauccaag aaacucaaga     3600

gcgucaaaga acugcucggg aucacgauua uggagcgcuc cucuuuugag aagaacccua     3660

ucgauuuccu ggaagcuaag ggauacaaag aggugaagaa agaccuuauu aucaagcugc     3720

ccaaauauag ccuguuugaa cucgagaaug gccggaagag aaugcuggcc uccgccgggg     3780

aacuucagaa gggaaacgag cuggcucucc cuucuaaaua cguuaauuuc cuguaucucg     3840

ccagccauua cgagaagcug aaaggcuccc ccgaagauaa cgagcagaag cagcuuuucg     3900

uggaacaaca caaacauuau cuggacgaga ucauugaaca gaucucugag uuuagcaagc     3960

gggucauucu ggcggaugcu aaucucgaca aagugcuguc cgccuacaac aagcaccgcg     4020

auaagccgau cagagagcag gccgaaaaca uuauccaccu uuucacccug acaaaucucg     4080

gggcuccugc cgcuuuuaaa uauuucgaca ccacaauuga ucggaagcgc uacaccagca     4140

cgaaagaggu ucuggacgcc acacucaucc aucaaucuau caccggccug uacgaaacca     4200

gaauugaucu uucccagcug ggaggcgaca agcggcccgc ggccacaaag aaggcugggc     4260

aggccaagaa aaagaaauaa ggacuaguga agggaacacc caaauuuaau ucagccuuaa     4320

gcacaauuaa uuaagaguga aacguaauug uacaagcagu uggucaccca ccauagggca     4380

ugaucaacac cgcaaccuuu ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu     4440

cagcuugcuu agauguucca aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa     4500

aaaaaggcca caaaaguucc cucucacuuu caguaaauaa aauaaaagca gcaacagaaa     4560

uaaagaaaua aaugaaauuc aaaaugaaau aaauauugug uugugcagca uuaaaaaauc     4620

aauaaaaauu aaaaaugagc aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               4716


<210>  4651
<211>  5499
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4651
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa cuucaagauu cugccgaucg cgauugaccu cggaguuaaa aauacaggcg      180

uguuuagcgc uuucuaccag aaggggacau cccuggagcg gcuugauaac aagaauggaa      240

aaguguauga acugucuaag gacagcuaca cgcuccugau gaacaaucgc accgccagac      300

ggcaucaacg cagaggcauc gaucggaaac agcuugucaa gcgccuguuu aaacucauuu      360

ggacagagca gcugaaccug gaaugggaca aggauaccca gcaagccauc uccuuccucu      420

uuaauagacg gggguucucu uuuaucacag acggauauag ccccgaguac cugaacauug      480

ugccugaaca gguuaaggcu auccuuaugg auauuuucga cgauuauaau ggcgaggacg      540

aucuggacuc cuaccucaaa cuggccaccg agcaggaauc uaagaucagc gagauuuaua      600

acaaacuuau gcagaagauc cuggaauuua aacucaugaa gcugugcaca gauauuaaag      660

acgacaaggu guccaccaag acgcugaaag agaucaccag cuacgaguuc gaacuccugg      720

cugauuaccu ugccaauuau ucugaguccc ugaagacaca aaaauuuagc uacaccgaca      780

agcagggcaa ccucaaagaa cugucuuauu accaccauga uaaguauaau auccaggagu      840

uccuuaagcg ccacgcgaca auuaacgaca gaauccugga uacccuccug acggacgauc      900

uggacauuug gaauuuuaac uucgaaaaau ucgauuuuga caagaacgag gagaaacucc      960

agaaucaaga agauaaggac cauauccagg cccaucugca ccacuucguc uuugcuguga     1020

acaaaauuaa guccgagaug gccagcgggg gacggcaccg cucucaguac uuccaagaaa     1080

ucacaaaugu ucuugacgag aacaaucauc aggaaggcua ucugaagaac uuuugugaga     1140

aucuccacaa caaaaaguac uccaaucuga gcgugaaaaa ccuugucaau cugaucggga     1200

accucuccaa ucuggagcug aagccccuca gaaaauacuu caacgauaag auucacgcua     1260

aggccgacca uugggaugaa cagaaauuua ccgagacaua uugccacugg auccugggag     1320

aauggcgggu gggcgugaag gaccaggaua aaaaggacgg ggcgaaauac ucuuauaagg     1380

aucuuuguaa ugagcugaaa caaaagguca ccaaggccgg acucguugac uuccugcugg     1440

aacuugaucc uugccgcacc auucccccuu accucgacaa caacaauaga aaaccgccca     1500

agugucagag ccugauccug aacccuaaau uucucgauaa ucaguauccc aacuggcagc     1560

aauaccugca ggagcuuaag aaacugcagu ccauucagaa uuaucucgac agcuucgaga     1620

cagaucugaa ggugcugaag ucuuccaaag accaaccuua cuuuguggaa uacaagagcu     1680

cuaaccagca gaucgcuagc ggccagcggg acuauaaaga ucuugacgcc agaauccucc     1740

aauucauuuu ugaucgcguc aaggcuuccg acgagcugcu gcucaaugaa aucuacuucc     1800

aggccaaaaa gcugaagcag aaagccucua gcgagcuuga gaagcuggaa uccagcaaaa     1860

agcucgauga ggugauugcu aacucucaac ugucccagau ccugaaaagc cagcauacga     1920

auggcauuuu cgaacagggg accuuucuuc accucguuug caaguauuac aagcaacggc     1980

agagagcgcg cgacucucgg cuguauauca ugcccgagua cagauaugau aaaaagcugc     2040

auaaauacaa caauacagga cgcuucgacg augacaacca gcuccugacc uacuguaauc     2100

acaagccgcg gcagaaaaga uaucaacuuc ugaacgaucu cgccggcgug cugcaggucu     2160

ccccuaauuu ucugaaggac aaaauuggga gcgaugacga ccuuuucauc uccaaguggc     2220

ucguggaaca cauccgcgga uuuaagaagg cuugcgagga uucucugaaa auucagaaag     2280

acaaccgggg ccugcucaac cauaagauca auauugccag aaacacaaaa gggaagugug     2340

agaaggaaau cuucaaucug auuugcaaaa ucgagggaag cgaagauaag aaaggcaacu     2400

acaagcacgg ccuugccuau gagcuggggg uucuccuguu uggagaaccc aaugaggcuu     2460

ccaaaccuga guucgaccgc aagaucaaga aauuuaacuc uauuuacagc uucgcccaga     2520

uccaacagau ugcguuugcu gaacggaagg gcaaugccaa caccugugcc gugugcuccg     2580

cugauaaugc ccauagaaug cagcaaauca aaauuacgga gcccgucgaa gacaacaagg     2640

auaaaaucau ccugagcgcu aaggcccagc ggcuucccgc gauuccuaca cgcaucgugg     2700

acggggccgu caagaaaaug gcuaccauuc uggccaagaa uaucguggau gacaacuggc     2760

agaauauuaa acagguucuc ucugcuaagc accaacugca caucccgauc auuaccgagu     2820

ccaacgccuu cgaauuugag cccgcgcucg ccgaugugaa aggaaagagc cugaaggaca     2880

gacggaaaaa ggcucuugag agaaucucuc cugaaaauau uuucaaagac aagaacaacc     2940

gcaucaaaga guucgccaag ggcauuuccg cuuauagcgg ggccaaucug acagauggcg     3000

acuuugaugg agccaaagag gaacucgacc auaucauucc ccggucucac aagaaguacg     3060

gcacccugaa cgaugaggcu aaucugaucu gcgucacaag aggggacaac aaaaauaagg     3120

gaaaccgcau cuucugucuu agagaucugg cggacaauua uaaacucaag caguuugaaa     3180

cgaccgauga ccuggagauu gagaaaaaga ucgccgauac aauuugggac gcuaacaaga     3240

aagauuucaa guuuggcaau uaccggagcu ucaucaaccu caccccucag gaacagaaag     3300

ccuuucgcca ugcccuguuc cuugcugacg aaaaucccau uaagcaagcc gugaucagag     3360

cgaucaacaa ucggaaccgc acauuuguua augggaccca gagauacuuc gcugaggugc     3420

uggccaacaa uauuuaucuc cgggccaaaa aggagaaccu gaacacggac aagaucuccu     3480

uugauuacuu cggcauuccg accaucggaa augggcgcgg cauugcugaa aucagacagc     3540

uguaugagaa agucgacagc gauauccagg ccuacgcgaa gggagacaaa ccucaggcuu     3600

cuuauuccca ccuuauugau gccaugcugg ccuucugcau cgcugccgac gaacaucgga     3660

acgauggcag cauugggcuc gagaucgaca agaauuacuc ccuguauccc cucgauaaaa     3720

acacaggaga aguguuuacc aaggacauuu ucucucaaau caagauuaca gauaaugagu     3780

uuagcgacaa aaagcuggug cgcaaaaagg cuaucgaggg cuucaacacc cacagacaga     3840

ugacgcggga ugggaucuac gccgaaaauu accuuccuau ucugauccac aaagagcuga     3900

acgaaguuag aaagggauau acauggaaaa auuccgagga aauuaagauc uuuaagggca     3960

aaaaguacga cauucagcaa cucaacaauc uggucuauug ucuuaaauuc guggacaagc     4020

ccaucucuau cgauauucag aucagcaccc uggaggagcu ccgcaacauu cugacaacca     4080

auaacaucgc ggccaccgcu gaauacuauu acauuaaucu caaaacacag aagcugcaug     4140

aguauuacau cgaaaacuac aacacggccc uuggguauaa gaaauacucc aaggagaugg     4200

aguuucugcg gucucuggcu uauagaagcg aacgcgugaa aaucaagucc auugacgaug     4260

ucaaacaagu gcucgacaag gauagcaauu ucaucauugg caagaucacc cugccuuuua     4320

aaaaggagug gcagcggcuu uacagagaau ggcagaacac aaccauuaaa gacgauuaug     4380

aguuccugaa gucuuucuuc aauguuaaau ccaucacaaa gcuccacaag aaagugcgca     4440

aggacuuuag ccugcccauu ucuaccaacg aaggaaaauu ccucgucaag cggaaaacgu     4500

gggauaauaa cuucaucuac cagauccuga augacuccga uagcagagcc gacggcacaa     4560

agccguuuau uccugccuuc gacaucucua aaaacgagau uguggaggcu aucauugauu     4620

ccuuuaccag caagaauauc uucuggcuuc ccaagaacau cgaacugcaa aaaguugaca     4680

auaagaacau uuuugcgauc gauaccucca aaugguucga gguggaaaca cccagcgacc     4740

ugcgcgauau ugggaucgcc accauucagu auaagaucga caauaacucu cggccuaaag     4800

ucagagugaa gcucgauuac guuaucgacg augacagcaa gauuaauuac uuuaugaacc     4860

auucccugcu uaaaucucgg uauccggaua aggugcugga gauccucaaa cagagcacaa     4920

uuaucgaauu ugaguccucu ggauuuaaca agacgauuaa agagaugcug ggcaugaagc     4980

ucgcugggau cuacaaugaa accagcaaca auaagcgccc cgccgccacc aaaaaggcug     5040

gacaggccaa aaagaaaaag uaaggacuag ugaagggaac acccaaauuu aauucagccu     5100

uaagcacaau uaauuaagag ugaaacguaa uuguacaagc aguuggucac ccaccauagg     5160

gcaugaucaa caccgcaacc uuuccuuuuu cccccaguga uucugaaaaa ccccucuucc     5220

cuucagcuug cuuagauguu ccaaauuuag uaagcuuaag gcggccuaca gaagaaaaag     5280

aaaaaaaagg ccacaaaagu ucccucucac uuucaguaaa uaaaauaaaa gcagcaacag     5340

aaauaaagaa auaaaugaaa uucaaaauga aauaaauauu guguugugca gcauuaaaaa     5400

aucaauaaaa auuaaaaaug agcaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5460

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            5499


<210>  4652
<211>  3771
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4652
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gcggaacuac auucugggac ucgacaucgg cauuaccagc guuggguaug      180

gaaucauuga uuacgagaca cgcgacguga ucgaugcggg cgugagacug uucaaagaag      240

cuaaugucga gaacaaugaa gggcggcgcu ccaagagagg agcccggcgc cuuaagagac      300

ggcgcagaca ucggauccag cgcgugaaaa agcugcucuu ugacuauaac cugcuuacgg      360

aucacucuga gcugagcggc auuaauccgu acgaagccag aguuaaaggc cucucccaaa      420

agcugucuga ggaggaguuc agcgcugccc ugcuccaucu ggcuaaacgg cgcggggugc      480

acaacgucaa ugaaguggaa gaggacaccg gaaacgagcu uuccacaaag gaacagaucu      540

cuagaaauag caaggcccug gaggaaaaau auguugcgga gcuccagcug gaacggcuua      600

agaaagaugg cgaggugaga ggguccauua accgcuuuaa gaccagcgac uacgucaaag      660

aggccaagca gcugcucaaa gugcaaaagg cuuaucauca gcuggaucag ucuuucaucg      720

acacauacau ugaucugcuc gaaacccgga gaacauacua ugagggaccc ggcgaagggu      780

ccccuuuugg auggaaggac aucaaagagu gguacgaaau gcugaugggc cacugcaccu      840

auuuccccga ggagcuucgc agcgugaagu acgccuauaa ugcugaucug uacaacgccc      900

ucaaugaccu gaacaaucuu gucauuacgc gggacgaaaa cgagaaacug gaauauuacg      960

agaaguuuca gaucaucgaa aacguuuuca aacaaaagaa gaaaccuacc cucaagcaga     1020

uugcgaaaga gauccuggug aaugaggaag auauuaaggg cuacagagug acaucuaccg     1080

ggaaacccga guuuacaaac cugaaggucu aucacgacau caaggauauu accgcccgca     1140

aagaaaucau cgagaaugcu gagcuccugg accagauugc caagauccuu acgauuuacc     1200

aguccagcga agauauccaa gaggaacuga caaaccucaa uucugagcug acccaggaag     1260

agauugagca gaucuccaac cuuaaaggau auacaggcac ccauaaucug agccucaagg     1320

cuaucaaccu gauucuggac gaacucuggc acaccaauga uaaccaaauc gccauuuuca     1380

aucggcugaa acuugugccu aagaagguug accuguccca gcagaaagag aucccgacaa     1440

cgcucgugga ugacuuuauu cugucucccg ucgugaagcg cagcuucauc caguccauua     1500

aaguuaucaa cgccaucauu aagaaauacg ggcugccuaa ugauaucauu aucgaacuug     1560

cucgcgagaa gaacagcaaa gacgcgcaaa agaugauuaa ugaaaugcag aagcggaaca     1620

gacagaccaa cgagcgcauc gaggaaauca uucggacaac cggaaaagag aaugccaagu     1680

aucucaucga gaaaauuaag cugcaugaca ugcaggaagg caaaugucug uacucucucg     1740

aagcuauccc ccuggaggau cuucugaaca auccuuuuaa cuaugaggug gaccacauua     1800

uccccagauc cgucagcuuc gauaauucuu ucaacaauaa ggugcucguc aagcaagaag     1860

agaacagcaa aaaggggaau cggacaccgu uucaguaccu guccucuagc gacuccaaaa     1920

ucagcuacga aaccuucaag aaacacauuc ugaaccuugc caagggaaag ggccgcaucu     1980

cuaaaacgaa gaaagaguau cuccuggagg aaagagauau uaaucgguuu uccgugcaga     2040

aggacuucau caacagaaau cugguugaua cacgcuacgc cacccggggc cucaugaacc     2100

ugcuuagaag cuauuuucgc gugaauaacc uggacgucaa agugaagucu auuaacgggg     2160

gauucaccuc cuuucucaga cggaagugga aauucaagaa agagcgcaau aagggcuaca     2220

aacaucacgc ugaagaugcc cugaucaucg cgaacgcuga cuuuauuuuc aaggagugga     2280

aaaagcugga uaaggccaaa aagguuaugg aaaaucagau guuugaggag aaacaagccg     2340

aaagcaugcc ugagaucgaa acagagcagg aauauaagga gauuuucauc acaccccauc     2400

agauuaaaca caucaaggac uuuaaggauu acaaauauuc ccauagagug gacaagaaac     2460

cuaaccggga gcuuauuaau gacacccucu acucuacgcg caaggaugac aaagggaaca     2520

cccugaucgu caauaaccug aauggacucu acgauaagga caacgauaag cugaagaaac     2580

uuaucaauaa aagccccgaa aagcugcuca uguaucacca cgacccccag acauaccaaa     2640

aacugaagcu gauuauggag caguauggcg augaaaagaa cccucuuuac aaauauuacg     2700

aggaaaccgg gaauuaucuc acaaaguacu ccaaaaagga caacggcccg gugaucaaaa     2760

agauuaagua cuauggaaau aaacugaacg cucaucugga uaucaccgac gauuacccca     2820

acucuagaaa uaaggugguu aaacucagcc ugaagccuua ucgguucgac gucuaccuug     2880

auaacggcgu guauaaauuc gugacgguca agaaucugga cgugauuaaa aaggagaacu     2940

acuaugaggu uaauuccaag ugcuacgaag aggccaaaaa gcucaaaaag aucagcaacc     3000

aggcugaauu uaucgccucu uucuacaaua acgaccugau uaaaaucaau ggggagcugu     3060

aucgcgugau uggagucaac aaugaucuuc ugaacagaau cgaggugaau augauugaca     3120

ucaccuaccg ggaauaucuc gagaacauga augauaagag acccccuaga aucauuaaga     3180

caaucgcguc caaaacccaa agcauuaaga aauacucuac agacauccug ggcaaccucu     3240

augaaguuaa guccaaaaag cacccccaga uuaucaagaa agggaagcgg ccggccgcua     3300

ccaaaaaggc cggacaggcu aaaaagaaga aauaaggacu agugaaggga acacccaaau     3360

uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa gcaguugguc     3420

acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu gauucugaaa     3480

aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua aggcggccua     3540

cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua aauaaaauaa     3600

aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua uuguguugug     3660

cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              3771


<210>  4653
<211>  4839
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4653
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcccu guucaacaag ugcauuauca uuagcaucaa ucucgacuuu uccaacaaag      180

agaaguguau gaccaagccg uacucuauug gacuggauau cggcacaaau agcguugggu      240

gggcggugau cacggacaac uauaaagugc ccuccaagaa aaugaagguc cuuggaaaua      300

ccucuaaaaa guacauuaag aaaaaccugc ucggcgugcu gcuuuucgau agcgggauca      360

cagcugaagg acggcgccug aagagaaccg cccggcgcag auauacacgg cgcagaaauc      420

ggauucucua ccugcaggag aucuuuucca ccgaaauggc cacacuggac gaugcuuucu      480

uucaacgccu cgacgauucu uuccugguuc cugacgauaa aagagacagc aaguauccca      540

uuuuuggcaa ccuuguggag gaaaaagucu accaugauga guucccuacc aucuaucauc      600

ugcggaagua ccucgccgac uccacgaaaa aggcugaucu gcgccuugug uaccuggccc      660

ucgcgcacau gauuaaguau agaggccacu uucugaucga gggggaguuc aauagcaaaa      720

acaaugacau ccagaagaac uuucaggacu uccuggauac cuacaaugcc auuuuugaau      780

cugaccucuc ccuggaaaac agcaaacagc uugaggagau cguuaaggau aaaauuucua      840

agcuggaaaa gaaagaccgg auccucaagc uguuccccgg agagaaaaau uccggcauuu      900

ucagcgaauu ucuuaagcug aucgugggga accaagcuga uuuccgcaaa ugcuuuaacc      960

ucgacgagaa ggccucucug cauuucucca aggaaagcua ugaugaggac cuggagacac     1020

uccugggaua caucggcgau gacuauuccg augucuuucu uaaagcuaag aaacuguacg     1080

acgccauucu ccugucuggg uuccuuaccg ugacagacaa ugaaaccgag gcgccucuga     1140

gcuccgccau gaucaagaga uauaacgaac acaaagagga ucucgcucug cugaaggaau     1200

acauucggaa uaucagccuc aagacguaca acgagguguu uaaagacgau acaaagaaug     1260

gauaugccgg cuacauugac ggcaaaacca accaggagga uuucuauguc uaccugaaga     1320

aucuucuggc ugaauuugag ggggccgacu auuuccucga aaagaucgau agagaggacu     1380

uucugcgcaa gcagcggaca uucgauaacg gaucuauccc guaccagauu caucugcaag     1440

aaaugagagc cauccuugac aaacaggcua aguuuuaucc cuuccucgcg aagaauaaag     1500

agcgcauuga gaagauccug accuuccgga uuccuuacua cguuggcccc cuggccagag     1560

ggaacuccga uuuugcuugg agcauccgca aacggaauga aaagauuacc ccuuggaacu     1620

ucgaggacgu gaucgauaaa gaaucuagcg ccgaggccuu uaucaauaga augacauccu     1680

ucgaccucua ucugcccgag gaaaaggugc uuccgaagca cucucugcuc uacgagacgu     1740

uuaacgucua uaaugaacug accaaagugc gcuucauugc ugagagcaug cgggacuacc     1800

aguuucugga uuccaagcag aaaaaggaca ucguuagacu uuauuucaaa gauaagcgca     1860

aggugacaga caaagauauu aucgaauacc uccacgccau uuauggauac gacggcaucg     1920

agcugaaggg gaucgagaaa caauuuaaca gcucucuguc caccuaccau gaucuccuga     1980

acauuaucaa ugacaaggag uuccuugaug acagcucuaa cgaagcgauu aucgaagaga     2040

uuauccacac acugaccauc uuugaagauc gggagaugau uaaacagaga cucuccaagu     2100

ucgagaauau cuuugacaag agcguccuga aaaagcuguc ccggcgccac uauacgggau     2160

ggggcaaacu uucugcuaag cucauuaacg gcaucagaga ugaaaagagc gggaauacaa     2220

uucuggacua ccugaucgac gauggaaucu ccaaccggaa uuucaugcag cucauucaug     2280

acgaugcccu gucuuucaag aaaaagaucc aaaaggccca gauuaucggc gacgaggaua     2340

aagggaacau uaaggaagug guuaaaagcc uuccuggauc ccccgcuauc aagaaaggca     2400

uucugcagag caucaagauc guggacgagc ucgucaaggu gaugggcggg agaaaaccug     2460

aaucuauugu cguggagaug gcccgcgaga aucaguauac caaccaagga aaguccaaua     2520

gccagcagcg gcugaaaaga cuggaaaagu cucuuaaaga gcucggcucc aagauccuga     2580

aggaaaacau ucccgcuaaa cugagcaaga ucgauaauaa cgcccuccag aaugaccgcc     2640

uguaccuuua uuaccugcaa aacgggaaag auauguauac cggagacgau cucgacauug     2700

acagacuguc uaacuacgau aucgaccaca ucauucccca ggcguuucug aaggauaaua     2760

gcaucgacaa caaaguucuu guguccagcg ccucuaaucg gggcaagucc gaugacuucc     2820

cuagccugga ggucgugaag aaacgcaaga cauuuuggua ccagcuccug aaauccaagc     2880

ucauuucuca gagaaaauuc gauaaccuga ccaaggcuga gcgcggggga cuucugccgg     2940

aagacaaggc cggcuuuauc caacggcagc ucguugagac aagacagauu acgaaacaug     3000

uggcucggcu gcuggaugaa aaguucaaua acaaaaagga cgagaauaac cgcgccguca     3060

gaaccgugaa aaucaucaca cuuaagagca cccugguguc ccaguuucgg aaagauuucg     3120

aacucuauaa gguucgcgag auuaaugacu uucaccaugc gcacgaugcc uaccugaacg     3180

cugucaucgc cucugcucuc cugaagaaau aucccaaacu ugagccugag uucguguacg     3240

gggacuaucc caaauacaau agcuuuagag aacggaaguc cgccacagaa aaaguguauu     3300

ucuacucuaa cauuaugaau aucuucaaaa agagcauuuc ccuggccgac ggcagaguca     3360

ucgagcgccc ucucauugaa gugaacgagg agaccggaga aagcguuugg aauaaagagu     3420

cugaucuggc uacggugcgg agaguccugu ccuaccccca agugaacguu gugaagaaag     3480

ucgaggaaca gaaccacggc cuugaccgcg ggaagccgaa aggacuguuu aaugcgaacc     3540

ucagcucuaa gccuaagccc aauuccaacg aaaaucuggu gggcgccaaa gaguaucucg     3600

auccuaagaa auacggggga uaugcuggca ucagcaacuc uuucgccguu cuggugaagg     3660

ggaccaucga gaaaggcgcc aagaagaaaa uuacaaaugu ccuugaauuu cagggaaucu     3720

ccauucugga ccggaucaac uacagaaagg auaaacugaa uuuccuccug gagaagggcu     3780

auaaagacau ugaacuuauc aucgagcugc ccaaguacag ccucuuugag cuguccgaug     3840

ggagccgccg gaugcucgcu ucuauucuga gcaccaacaa uaaaagagga gaaauccaua     3900

agggcaacca aauuuuccuu ucccagaagu uugugaaacu gcuguaucac gccaagcgca     3960

ucucuaauac aauuaacgag aaucaucgga aauacgugga aaaccacaag aaagaguucg     4020

aggaacucuu uuacuauauc cuggaguuca augaaaacua cgucggggcg aagaagaacg     4080

gaaaacuucu gaauagcgcu uuucaguccu ggcagaacca cucuaucgac gagcucugua     4140

gcuccuucau uggcccuacc gggucugaaa gaaagggccu guuugagcuc acgagccggg     4200

gauccgccgc cgauuucgag uuccugggcg uuaaaauccc ccgcuauaga gacuacacac     4260

cgagcucucu ucugaaggau gcuacccuga uucaucaauc cgugacaggg cucuaugaaa     4320

cccggaucga ccuggccaaa cuuggagagg gcaagcgccc ugcggcuacc aagaaagccg     4380

ggcaggccaa gaaaaagaaa uaaggacuag ugaagggaac acccaaauuu aauucagccu     4440

uaagcacaau uaauuaagag ugaaacguaa uuguacaagc aguuggucac ccaccauagg     4500

gcaugaucaa caccgcaacc uuuccuuuuu cccccaguga uucugaaaaa ccccucuucc     4560

cuucagcuug cuuagauguu ccaaauuuag uaagcuuaag gcggccuaca gaagaaaaag     4620

aaaaaaaagg ccacaaaagu ucccucucac uuucaguaaa uaaaauaaaa gcagcaacag     4680

aaauaaagaa auaaaugaaa uucaaaauga aauaaauauu guguugugca gcauuaaaaa     4740

aucaauaaaa auuaaaaaug agcaagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            4839


<210>  4654
<211>  3915
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4654
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccug guacgcgagc cugauguccg cucaucaccu ccggguugga auugacgugg      180

gcacccauuc uguggggcug gccacacuuc gcgucgauga ccacggaacg ccgaucgagc      240

ugcucagcgc ccugucccau auucacgauu cuggcguggg gaaggaaggg aaaaaggacc      300

acgauaccag aaagaaacuu agcggcaucg cucggcgcgc cagacggcug cuccaucacc      360

gcagaacaca gcugcaacag cuggacgagg uucuccggga ucugggcuuc cccauuccua      420

cccccgggga auuucuugac cugaacgagc agacagaucc uuaucgcgug uggagagucc      480

gggcucgccu cguggaagag aagcugcccg aggaacuuag aggaccugcc aucuccaugg      540

cgguucggca uaucgcccgc cacagaggcu ggcggaaucc guacucuaaa guggagagcc      600

ugcucucccc cgcugaagag agcccuuuca ugaaggcccu gagagagcgc auucuggcua      660

ccacagggga aguccucgac gauggaauca cccccggcca ggccauggcg caaguggccc      720

ugacgcacaa cauuucuaug cgggggccug agggaauccu uggcaaacug caucaguccg      780

acaaugcuaa cgaaauuaga aagaucugcg cccgccaggg cgugagcccc gaugucugua      840

agcagcuccu gcgggcuguu uuuaaagccg acucuccgag aggguccgcc gugagccgcg      900

uggcuccuga cccccuuccu ggacaaggcu cuuuccggag agcgcccaag ugcgaucccg      960

aguuucagcg cuuccggauu aucuccaucg ucgccaaucu gagaauuagc gaaaccaaag     1020

gggagaaccg cccucucaca gcugacgagc ggagacacgu gguuaccuuu cugacagaag     1080

auucccaggc cgaccugacc uggguggaug ucgccgagaa gcucggagug caucggcgcg     1140

accugagagg cacggcuguu cacacagaug acggggaacg gucugccgcg agaccgccca     1200

ucgaugcuac cgaccgcauu augcggcaga caaaaaucag cucccuuaag accugguggg     1260

aggaagccga uagcgagcaa agaggagcca ugauucgcua ucuguacgag gacccuaccg     1320

acucugaaug ugcugagauc aucgccgaac uccccgagga agaucaggcu aaacuggacu     1380

cccuucaucu gccugccggc agagcggccu auagccggga gucucucaca gcucugagcg     1440

aucacaugcu ggccacgacc gacgaucucc acgaggcucg caagagacug uucggcgugg     1500

acgauuccug ggcgcccccg gccgaagcca uuaaugcucc ugucgggaac cccucugugg     1560

accggacacu uaagaucguc ggacgcuacc ugagcgccgu ggaguccaug uggggcaccc     1620

cugaaguuau ucauguggag cacgucagag auggguuuac aagcgagcgg auggcugacg     1680

aacgcgauaa agccaauaga cggcgcuaua acgacaauca ggaggccaug aagaaaaucc     1740

aaagagauua cggaaaggaa ggcuacauuu cucggggaga caucgugaga cucgacgcuc     1800

uggagcugca gggcugcgcg ugucuuuauu gcggaaccac gaucggcuac cauacauguc     1860

agcucgauca cauuguuccc caggccgggc cuggauccaa caaucgccgg ggcaaccugg     1920

uggcugucug cgaaagaugu aaucgcagca aaucuaacac ccccuucgcc gugugggccc     1980

aaaagugcgg gaucccgcac gugggaguua aggaggcuau uggccggguc agaggguggc     2040

gcaaacagac cccuaacaca uccagcgagg accugacccg gcucaagaaa gaagugaucg     2100

ccagacugcg ccggacacag gaggaucccg aaauugacga gagauccaug gaaucugugg     2160

cguggauggc uaaugagcuu caucaccgga ucgccgccgc uuaucccgag acgaccguca     2220

ugguguaccg cggcagcauu acagccgcgg cuagaaaggc cgccggaauc gauucccgga     2280

ucaaccugau uggcgaaaaa gggcgcaagg acagaaucga ucggagacau cacgcuguug     2340

acgccucugu ggucgcucuc auggaggcca gcguggcgaa gacccuggcc gaacgcucca     2400

gccugcgggg agagcagaga cuuacaggca aagaacaaac cuggaagcag uauacggggu     2460

cuaccguugg agcucgcgag cauuuugaga uguggcgggg ccacaugcuc caccugacag     2520

aacuguucaa ugagagacuc gccgaagaua aaguguacgu cacccagaac auucgccugc     2580

ggcuuuccga cgggaaugcu cauacaguga acccuagcaa gcugguuucu cacagacucg     2640

gcgauggacu gaccgugcag caaaucgacc gcgccuguac gccggcccug uggugcgcuc     2700

uuacaagaga gaaagauuuu gacgagaaga auggccuccc cgcgcgggaa gaccgcgcca     2760

uuagagucca ugggcacgag aucaagucca gcgauuauau ccagguguuc ucuaaacgga     2820

agaaaaccga cagcgaucgc gacgaaacac cuuucggagc uauugccgug agaggcgggu     2880

uugucgagau cggacccucc auucaucacg cccggaucua cagaguugaa ggcaagaaac     2940

cuguguacgc uaugcugcgc guguucaccc acgaucugcu cagccagcgg cauggggacc     3000

uguuuucugc cgucauuccc ccucagucca ucagcaugag augcgcggag cccaaacuuc     3060

gcaaagcuau caccacaggc aacgccacgu aucugggaug ggugguugug ggcgaugagc     3120

ucgaaauuaa ugucgacucc uucaccaagu acgccaucgg gcgguuucug gaggauuucc     3180

cgaacacaac cagauggcgg auuuguggau augacacaaa uucuaagcug acccuuaaac     3240

cuaucgugcu cgcugccgaa ggccuggaga accccagcuc cgcgguuaau gaaauugugg     3300

agcugaaggg guggcgcguc gcuaucaacg ugcucacgaa aguucacccu acagugguca     3360

gacgggaugc ccugggacgc cccagauacu cuagccgguc caaucuuccg accucuugga     3420

ccaucgagaa gcgcccugcc gcuacaaaga aggccggcca agcuaagaaa aagaaauaag     3480

gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau uaagagugaa     3540

acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc gcaaccuuuc     3600

cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa     3660

auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc     3720

ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa augaaauuca     3780

aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua aaaaugagca     3840

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3900

aaaaaaaaaa gaauu                                                      3915


<210>  4655
<211>  4776
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4655
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccac caagccguac agcauuggac uggacaucgg cacaaacucc guuggguggg      180

cggugacgac cgauaauuau aaagugcccu cuaagaagau gaaaguccuc ggaaacacaa      240

gcaagaaaua cauuaagaaa aaucugcuug gcgugcuccu guucgacucc gggaucaccg      300

cugagggacg gcgccugaag agaacagccc ggcgcagaua uacccggcgc agaaaccgga      360

uucuuuaccu gcaggaaauc uuuucuacag agauggccac ccucgaugac gcuuucuuuc      420

aacgccugga ugacagcuuc cugguuccug augacaagag agauuccaaa uaucccaucu      480

uuggcaaucu cguggaagag aaggccuacc augacgaguu cccuacgauu uaucaucugc      540

ggaaauaccu ugcugauucu accaagaaag ccgaccugcg ccucgucuac cuggcgcuug      600

cccacaugau caaguauaga ggccacuuuc ugauugaggg ggaguucaac agcaaaaaua      660

acgauaucca gaagaauuuu caggacuucc ucgacacaua caacgcuauu uuugaauccg      720

aucugagccu ggaaaauucu aagcagcucg aagagaucgu gaaagacaag auuuccaaac      780

uggagaagaa agaucggauc cuuaagcugu uccccggaga aaagaacagc ggcaucuuuu      840

cugaguuccu caaacugauu guugggaauc aagccgacuu ccgcaagugc uuuaaccuug      900

augaaaaggc uucccugcau uucagcaaag agucuuauga cgaagaucuc gagacccugc      960

ugggauacau cggcgacgau uauuccgacg uguuucucaa agccaagaag cuguacgaug     1020

cgauucuucu gagcggguuc cucacaguca ccgacaauga gacggaagcc ccucuguccu     1080

cugcuaugau caaaagauau aacgagcaca aggaagaccu ugcccugcuc aaagaguaca     1140

uucggaacau cagccugaag acauacaaug aaguguuuaa agaugacacc aagaacggau     1200

augcuggcua caucgauggc aagacaaauc aggaggacuu cuauguguac cugaaaaagc     1260

uccuggccga guuugaaggg gccgauuauu uccuugagaa aauugacaga gaagauuuuc     1320

ugcgcaagca gcggaccuuc gacaacggau ccaucccgua ccagauucac cuccaagaga     1380

ugagagcuau ccuggauaaa caggcgaagu uuuaucccuu ccuggccaaa aauaaggaac     1440

gcauugagaa gauccuuacc uuucggaucc cuuacuacgu cggcccccuc gcuagaggga     1500

acagcgacuu cgccuggucu auucgcaaac ggaaugagaa gaucacaccu uggaacuucg     1560

aagauguuau ugacaaagag uccagcgccg aggcuuuuau caauagaaug acgucuuucg     1620

aucuguaucu gcccgaagag aaggugcucc cgaaacauag ccugcuuuac gaaaccuuua     1680

acguguauaa ugagcugaca aagguccgcu ucauugccga auccaugcgg gacuaccagu     1740

uucucgacuc uaagcagaaa aaggauaucg ugagacugua uuucaaagac aagcgcaaag     1800

uuaccgauaa ggacauuauc gaguaccugc acgcgaucua uggauacgau ggcauugaac     1860

uuaaggggau cgagaaacaa uuuaacagcu cccucagcac auaccaugac cugcugaaua     1920

uuaucaacga uaaggaguuc cucgacgauu cuuccaauga agcuauuauc gaggaaauca     1980

uucacacccu gacgaucuuu gaggaccggg aaaugauuaa acagagacuu agcaaguucg     2040

agaacaucuu ugauaaaucu gugcugaaga agcucucccg gcgccacuau acaggauggg     2100

gcaaacugag cgccaagcug auuaacggca ucagagacga gaaauccggg aauaccaucc     2160

uugauuaccu cauugacgac ggaaucucua accggaauuu caugcagcug auucaugaug     2220

acgcccugag cuuuaagaaa aagauccaaa aagcucagau uaucggcgau gaagacaagg     2280

ggaacaucaa ggaggucgug aaaucccucc cuggaucucc cgccauuaag aaaggcaucc     2340

ugcagagcau uaagaucguu gaugaacuug ugaaagucau gggcgggaga aagccugagu     2400

ccauuguggu cgaaauggcu cgcgagaauc aguauaccaa ccaaggaaag agcaauucuc     2460

agcagcggcu gaaaagacuc gagaaguccc ugaaagaacu gggcagcaag auccuuaaag     2520

agaacauucc cgccaagcuc ucuaagaucg acaauaacgc gcugcagaau gaucgccugu     2580

accucuauua ccugcaaaac gggaaagaca uguauacagg agaugaccuu gauaucgaca     2640

gacuguccaa uuacgacauu gaucacauca uuccccaggc cuuccucaag gacaacagca     2700

ucgauaauaa agugcugguu ucuagcgcuu ccaaccgggg caagagcgac gaugugccuu     2760

cucuggaagu cgugaaaaag cgcaagaccu ucugguacca gcuucugaaa uccaagcuca     2820

uuagccagag aaaauuugac aaccugacaa aggccgagcg cgggggacuc uccccggagg     2880

auaaagcugg cuucauccaa cggcagcugg uugaaacgag acagaucacc aagcaugugg     2940

cccggcuucu ggacgagaag uuuaauaaca aaaaggauga aaauaaccgc gcggucagaa     3000

cagugaaaau uaucacccuc aagucuacac uggugagcca guuccggaaa gacuuugagc     3060

uguauaaggu ucgcgaaauu aaugauuucc accaugccca cgacgcuuac cuuaacgccg     3120

ucguggcuuc cgcccugcuc aaaaaguauc ccaagcugga gccugaguuu guguacgggg     3180

auuaucccaa auacaauucu uucagagaaa gaaagagcgc caccgagaaa gucuauuuuu     3240

acuccaacau caugaauauu uucaagaaau cuaucagccu cgcugacggc cgggugaucg     3300

aacgcccucu gauugagguu aacgaagaga cgggagaguc cguguggaau aaggaaagcg     3360

accuugcgac cguccggaga gugcugucuu acccccaagu uaacgugguc aagaaagugg     3420

aggaacagaa ucacggccuc gaucgcggga agccgaaagg acuguuuaac gccaaucugu     3480

ccagcaagcc uaaacccaac ucuaacgaga aucuuguugg cgcuaaggaa uaucuggacc     3540

cuaagaaaua cgggggauau gccggcaucu ccaacagcuu cacagugcuc gucaagggga     3600

ccauugagaa aggcgccaag aaaaagauca caaaugugcu ggaguuccag ggaauuucua     3660

uccucgaucg gauuaacuac agaaaggaca aacugaauuu ucuucuggaa aagggcuaua     3720

aagauaucga gcugaucauu gaacucccca aguacucccu guucgagcuu agcgacgggu     3780

cccgccggau gcuggcuagc auccucucua ccaacaauaa aagaggagag auucauaagg     3840

gcaaccaaau cuuucugagc cagaaauucg ugaagcuccu guaucacgcc aagcgcauuu     3900

ccaauacgau caacgaaaau caucggaaau acgucgagaa ccacaagaaa gaauuugagg     3960

aacuuuucua cuauauccug gaguuuaaug agaacuacgu uggggcgaag aaaaauggaa     4020

agcugcucaa cucugcuuuc cagagcuggc agaaucacuc cauugaugaa cuguguucua     4080

gcuuuaucgg cccuacaggg uccgagagaa agggccuuuu cgaacugacc ucucggggaa     4140

gcgccgccga cuuugaguuc cucggcguga aaauuccccg cuauagagau uacacaccgu     4200

ccagccugcu caaggacgcu acccugaucc aucaaucugu gaccgggcuu uaugaaacac     4260

ggauugaucu ggccaaacug ggagagggca agcgcccugc ggcuacgaaa aaggccgggc     4320

aggccaagaa aaagaaauaa ggacuaguga agggaacacc caaauuuaau ucagccuuaa     4380

gcacaauuaa uuaagaguga aacguaauug uacaagcagu uggucaccca ccauagggca     4440

ugaucaacac cgcaaccuuu ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu     4500

cagcuugcuu agauguucca aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa     4560

aaaaaggcca caaaaguucc cucucacuuu caguaaauaa aauaaaagca gcaacagaaa     4620

uaaagaaaua aaugaaauuc aaaaugaaau aaauauugug uugugcagca uuaaaaaauc     4680

aauaaaaauu aaaaaugagc aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               4776


<210>  4656
<211>  3858
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4656
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc ggcuuucaag ccgaacagca uuaauuacau ccugggacuc gacauuggca      180

ucgccuccgu ugggugggcc augguggaga uugaugaaga ggaaaacccc auccggcuga      240

ucgaccuugg agugcgcguc uuugagagag cugaagugcc uaaaaccggc gauucucugg      300

ccauggcucg gcgccucgcc agaagcguuc ggcgccugac aagacggcgc gcgcauagac      360

uucugcggac gcgcagacuc cugaagcggg agggggugcu gcaggccgcu aauuucgacg      420

agaacggacu cauuaagucc cugcccaaua ccccuuggca acuucgcgcc gcugcccugg      480

auagaaaacu cacaccccug gaauggucug cgguccuucu gcaccucauc aagcaucggg      540

gcuaucugag ccagcgcaaa aacgagggcg aaaccgccga caaggagcug ggggcucucc      600

ugaaaggagu ggccggcaau gcucacgccc uucagacagg ggauuuuaga accccugccg      660

agcuggcucu caacaaguuc gaaaaggagu ccggacauau ucggaaucag agaucugacu      720

acagccacac auuuucccgc aaagaucugc aagcggaacu uauccuccug uucgagaagc      780

agaaagaauu uggcaacccg cacguuagcg ggggacugaa ggagggcauu gagacccugc      840

ucaugacgca gcggcccgcc cugucuggcg acgcugugca gaaaaugcuu gggcauugca      900

ccuucgaacc ugccgagccc aaggccgcua aaaauacaua uaccgccgaa agauuuaucu      960

ggcugacaaa gcucaacaau cugcgcauuc uugagcaagg auccgaacgg ccucugaccg     1020

auacggagag agcgacacuc auggacgagc ccuaccgcaa gagcaaacug accuaugcuc     1080

aggcccggaa gcugcucggc cuggaagaua cagccuucuu uaaagggcuu agauacggaa     1140

aggacaacgc ugaggccucu acccugaugg aaaugaaggc uuaucacgcc aucucccgcg     1200

cgcucgagaa ggaaggccug aaggacaaaa agagcccgcu gaaucuuucu ccugagcucc     1260

aggaugagau cgggaccgcc uucucccugu uuaaaacaga cgaagauauu acgggacggc     1320

ugaaggacag aauccagccc gagauucucg aagcucugcu uaaacauauc agcuucgaua     1380

aguucgucca aauuucccug aaggcccucc ggcgcaucgu gccucugaug gagcagggca     1440

aaagauacga cgaggcuugu gccgaaaucu acggcgauca cuaugggaag aaaaacaccg     1500

aggaaaagau uuaccugccu cccauccccg cggacgagau ucggaacccg gugguccuua     1560

gagcccucuc ucaggcucgc aaaguuauca auggaguggu gcggagauau ggcagccccg     1620

cccgcauuca caucgaaaca gcuagagagg ucgggaaguc cuuuaaggau cggaaagaga     1680

ucgaaaagcg ccaagaggaa aacagaaaag accgggagaa ggccgccgcu aaauuccgcg     1740

aauacuuucc uaauuucgug ggagagccca agagcaaaga uauucugaag cugagacucu     1800

augagcagca gcauggcaag ugccuguacu cugggaaaga aaucaaccuu ggccggcuga     1860

augagaaggg auauguugaa auugaccacg cgcucccuuu uucccgcacc ugggacgaua     1920

gcuucaacaa uaaagugcug guccugggcu cugagaacca gaauaagggg aaccaaacac     1980

ccuacgaaua cuuuaaugga aaagacaaca gcagagagug gcaggaguuc aaggcccggg     2040

uggaaaccuc ccgcuuuccg agaucuaaga aacagcggau ccuucuccag aaguucgaug     2100

aggacggcuu uaaagaaaga aaucugaacg auacgcgcua uguuaaucgg uuccuguguc     2160

aauuuguggc ugacagaaug cgccucacag ggaagggcaa aaagcggguc uucgccagca     2220

acggacagau uaccaaccug cuuagagggu ucuggggccu gcgcaaggug cgggccgaga     2280

augauagaca ucacgcucuc gacgccgucg ugguugcgug cuccaccgug gcuaugcagc     2340

agaaaaucac acgcuuuguc cgguacaagg agaugaacgc cuucgaugga aaaaccauug     2400

acaaggaaac aggcgaggug cugcaucaga aaacgcacuu uccucagccc ugggaguucu     2460

uugcccagga aguuaugauc agaguguucg ggaagccuga uggaaagccc gaauuugagg     2520

aggcugacac ccuggaaaaa cuucggacac uccuggccga gaagcugagc ucucgcccug     2580

aagcggucca cgaguaugug acaccccucu ucguguccag agcuccgaau cggaaaauga     2640

gcggccaggg gcauauggaa accguuaagu cugccaaacg ccuggaugag ggagucuccg     2700

ugcuuagagu gccucugacc caacucaagc ugaaagaccu ggagaagaug gucaaccggg     2760

aaagagagcc caaacuuuac gaagcccuga aagcucgccu cgaggcccac aaggacgauc     2820

ccgcuaaagc cuuugcggaa ccuuucuaua aguacgacaa agccggcaau cggacgcaac     2880

aggugaaggc uguuagagug gagcaagucc agaagaccgg gguguggguu cgcaaccaua     2940

auggcaucgc cgauaacgcu acaauggugc gggucgacgu guuugagaaa ggagauaagu     3000

auuaccuggu uccgauuuac agcuggcaag uggccaaagg cauccucccc gacagagccg     3060

ucgugcaggg gaaggaugaa gaggacuggc agcugauuga ugacuccuuc aauuuuaaau     3120

ucucucuuca cccuaacgau cugguggaag ucaucaccaa gaaggcucgc auguucggau     3180

auuuugcgag cugucaccgg ggcacaggga auauuaacau cagaauccau gaccucgauc     3240

acaaaauugg aaagaauggc auccuggagg ggauuggcgu uaaaaccgcc cuguccuucc     3300

agaaguacca gaucgacgag cuuggaaaag aaauucgccc cugcagacug aagaagcggc     3360

cucccgugcg caaaagaccu gcugccacga agaaagccgg ccaggcuaag aaaaagaagu     3420

aaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu aauuaagagu     3480

gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac accgcaaccu     3540

uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc uuagauguuc     3600

caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu     3660

cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau     3720

ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga     3780

gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaagaauu                                                   3858


<210>  4657
<211>  4614
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4657
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gaaaccguac accauuggac uggacaucgg cacaaacagc guuggguggg      180

cggugcucac ggaucaguau gaccugguga agcggaagau gaaaauugcu ggagauuccg      240

agaagaaaca aaucaagaaa aauuucuggg gcguccgccu uuuugacgaa gggcagaccg      300

ccgccgauag acggauggcu cgcacagcca gacggcgcau ugagagacgg cgcaacagaa      360

ucucuuaccu gcagggaauc uucgcugaag agaugagcaa gaccgacgcc aauuucuucu      420

gccggcucuc cgauucuuuu uauguggaca acgaaaagcg caauagcaga caucccuucu      480

uugcgacaau ugaggaggaa guugaguacc acaaaaacua uccuaccauc uaccaucugc      540

gggaggaacu ugucaauucc ucugagaagg ccgaucugcg ccucgucuau cuggcucugg      600

cccacauuau caaauacaga ggcaacuucc ucauugaagg cgcucuggac acacagaaua      660

ccagcgugga ugggaucuac aagcaauuua uucagacgua uaaccagguu uucgccuccg      720

gaaucgagga cggcagccuu aaaaagcugg aagauaauaa agacguggcg aagauccucg      780

ucgagaaggu gacccggaaa gaaaagcugg agcgcauucu uaaacuguac cccggggaga      840

agucugccgg aauguuugcu caguucaucu cccucauugu gggcagcaaa gggaacuuuc      900

aaaagccuuu cgaccugauc gaaaagucug auauugagug ugccaaagac uccuaugaag      960

aggaucugga aagccuccug gcucuuaucg gagacgagua cgccgagcug uucgucgccg     1020

cuaagaaugc guauucugcc guugugcucu ccagcaucau uacaguggcu gaaaccgaga     1080

caaacgccaa acuguccgcc ucuaugaucg aaagauuuga uacccaugag gaagaccuug     1140

gcgagcugaa ggcuuucauu aaacuccauc ugcccaagca cuacgaggaa aucuuuagca     1200

auacggagaa gcacggcuau gccggguaca uugauggaaa aacaaagcag gcggacuucu     1260

acaaauauau gaagaugacc cuggaaaaca ucgagggcgc ugauuacuuu aucgccaaaa     1320

uugagaagga aaacuuccuc cggaagcaga gaacauuuga caauggggcc aucccucacc     1380

agcugcaucu ugaggaacug gaggcuauuc uccaccaaca ggccaaauau uacccguucc     1440

ugaaggaaaa cuaugauaaa aucaaguccc uugucaccuu ucgcauuccc uacuucgugg     1500

gaccucuggc uaauggccag agcgaguuug ccuggcucac ccggaaagcg gacggggaga     1560

ucagacccug gaacauugaa gagaagguug acuucggaaa aucugccgug gauuuuaucg     1620

aaaagaugac aaauaaggac acguaucugc cuaaagagaa cguccugccc aagcacuccc     1680

ucugcuacca aaaauaccug guguauaaug aacuuaccaa gguucgcuac aucaacgauc     1740

agggcaaaac aagcuauuuc ucuggccagg agaaggagca gauuuucaau gaccuguuua     1800

agcaaaaacg gaaggugaaa aagaaagauc ucgaacuguu ccugagaaac augagccaug     1860

ucgagucccc gaccaucgaa gggcuugagg acucuuuuaa uagcuccuac agcacauauc     1920

acgaucuccu gaagguggga auuaagcagg aaauccugga caacccuguc aauaccgaga     1980

ugcucgagaa cauugugaaa auccugacgg uuuucgaaga uaagcgcaug aucaaagagc     2040

agcuucagca auuuucugac gugcuggaug gcgucgugcu caagaaacug gaacggagac     2100

auuacacagg guggggacgc cuguccgcua agcuccucau gggcauucgg gacaagcaga     2160

gccaccugac cauccuggau uaucucauga augacgacgg gcugaacaga aaccuuaugc     2220

agcugauuaa ugauucuaac cucuccuuca aaagcaucau ugagaaggag cagguuacca     2280

cagccgacaa agauauccaa uccaucgugg cugaccuggc cggcucuccc gcgauuaaga     2340

aaggaauccu gcagagccuu aagauugucg augaacucgu guccgugaug ggcuacccuc     2400

cccagaccau cguugucgag auggcccggg aaaaucagac aacggggaaa ggaaagaaca     2460

auucucgccc cagauacaag agccuggaga aagcuauuaa ggaauuuggc ucccaaaucc     2520

ugaaagagca uccuaccgac aaccaggagc uccggaauaa cagacuguau cuuuacuauc     2580

ugcagaaugg gaaggauaug uacacaggac aagaccucga uauccacaac cugagcaauu     2640

augacauuga ucacaucgug ccgcagucuu ucauuaccga caacuccauc gauaaucugg     2700

ugcuuacaag cucugccggc aaccgcgaaa aaggggacga cgugcccccu cucgagauug     2760

ugcggaagag aaagguguuu ugggaaaaac uguaccaggg caaucugaug uccaagcgca     2820

aauucgauua ucucaccaag gcugagagag gaggccugac ggaagccgac aaagcccggu     2880

uuauccaucg ccagcuuguu gagaccagac aaauuacaaa gaacgucgcu aacauccugc     2940

accagcgguu caauuacgag aaggaugacc augggaacac caugaaacag gugcgcaucg     3000

uuacacucaa gagcgcgcug gugucucagu uuagaaaaca auuccagcug uacaaggucc     3060

gggaugugaa ugacuaucac cacgcccaug augcuuaccu uaacggaguu guggccaaua     3120

cccugcucaa agucuauccc cagcuggaac cugaguucgu guacggcgac uaucaccagu     3180

uugauugguu caaggccaac aaggcuacgg ccaaaaagca auuuuacaca aauauuaugc     3240

uuuucuuugc gcagaaagac cgcaucauug augaaaacgg ggagauccug ugggacaaga     3300

aauaucuuga caccgugaag aaggucauga gcuacagaca gaugaauauu guuaaaaaga     3360

cagaaaucca aaaaggagag uucuccaagg cuaccaucaa acccaagggc aacagcucua     3420

aacugauucc gcggaagacc aauugggauc cuaugaagua cgggggccuc gacuccccca     3480

acauggccua ugccguggug aucgaguacg cuaaaggaaa gaauaaacug gucuuugaaa     3540

agaaaauuau ccgcgugaca auuauggaga gaaaggccuu cgaaaaggau gagaaagcgu     3600

uucuggagga acagggcuau cggcagccua agguucuugc uaaacugccc aaguacacgc     3660

ucuaugagug ugaagagggg agacgccgga ugcuggccag cgccaacgaa gcucagaaag     3720

gaaaucaaca ggugcucccu aaccaucugg ucacccuucu gcaccaugcc gcuaacugcg     3780

agguguccga cggcaagucu cucgauuaca ucgagagcaa uagagaaaug uucgccgagc     3840

ugcuggcgca cguuuccgaa uuugccaagc gcuauacacu ugcugaggcc aaccugaaua     3900

aaaucaacca gcucuuugaa cagaauaagg agggggacau uaaagcuauc gcccaaucuu     3960

ucguggaucu gauggccuuu aacgcuaugg gagcgcccgc cagcuucaag uuuuucgaga     4020

ccacaauuga acggaaaaga uacaauaacc ucaaggagcu gcuuaauucc accaucauuu     4080

accagucuau cacgggccug uaugaaagcc gcaagcggcu ggacgauaaa agaccggcug     4140

ccacaaagaa agccgggcag gcuaagaaaa agaaauaagg acuagugaag ggaacaccca     4200

aauuuaauuc agccuuaagc acaauuaauu aagagugaaa cguaauugua caagcaguug     4260

gucacccacc auagggcaug aucaacaccg caaccuuucc uuuuuccccc agugauucug     4320

aaaaaccccu cuucccuuca gcuugcuuag auguuccaaa uuuaguaagc uuaaggcggc     4380

cuacagaaga aaaagaaaaa aaaggccaca aaaguucccu cucacuuuca guaaauaaaa     4440

uaaaagcagc aacagaaaua aagaaauaaa ugaaauucaa aaugaaauaa auauuguguu     4500

gugcagcauu aaaaaaucaa uaaaaauuaa aaaugagcaa gaucuaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           4614


<210>  4658
<211>  4647
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4658
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gaaaccguac agcauuggac uggacaucgg caccaacucc guuggguggg      180

cgguggugac agaugacuau aagguccccg cuaagaaaau gaaggugcuc ggaaauacgg      240

auaaaucuca uauugagaag aaccugcuug gcgcccuccu guucgacagc gggaauaccg      300

ccgaagaucg gcgccugaaa agaacagcuc ggcgcagaua cacccggcgc agaaaccgga      360

uccuuuaucu gcaggagauu uucuccgaag agaugggaaa gguugacgau ucuuucuuuc      420

accgccucga agacagcuuc cuggugacag aggauaagag aggcgagcgg cauccuaucu      480

uuggcaaucu ggaagaggaa gucaaauacc acgagaacuu ccccaccauc uaucaucucc      540

gccaauaccu ggccgacaau ccugagaagg uggaucuuag acugguuuau cucgcucugg      600

cccacauuau caaauuucgg gggcacuucc uuauugaagg aaaguuugac acacgcaaca      660

augaugugca gagacuguuc caggaguuuc ucgcggucua cgacaacacc uucgaaaauu      720

ccucucugca ggagcaaaac guccaggugg aagagauccu gacggacaaa auuagcaagu      780

ccgccaaaaa ggaucgggug cucaagcugu uucccaauga gaaaagcaac ggcagauucg      840

cugaauuucu uaagcugauc guugggaauc aggccgacuu uaaaaagcau uucgagcucg      900

aagagaaagc uccucugcag uuuucuaagg auaccuacga agaggagcuu gaagugcugc      960

ucgcccaaau uggagacaac uaugcggagc uguuccuguc cgccaagaaa cucuacgaua     1020

gcauccugcu uucuggcauc cugacaguga ccgacgucgg gacaaaggcu ccgcucuccg     1080

ccagcaugau ucagcgcuau aacgaacacc agauggaucu ggcucagcuu aaacaauuua     1140

uccggcagaa gcugucugac aaauacaaug agguguucuc cgauguuagc aaggacggau     1200

augccggcua cauugauggc aagaccaacc aggaagccuu uuauaaauac cucaaggggc     1260

ugcugaauaa aaucgaggga uccggcuacu uccucgacaa gauugagaga gaagacuuuc     1320

ugcgcaaaca acggacguuc gauaacgggu cuauccccca ucagauccac cuucaggaga     1380

ugagagcuau uauccgccgg caggcggaau uuuauccuuu ccuggccgac aaucaagaua     1440

gaauugagaa gcuccugaca uuucgcaucc ccuacuaugu gggaccucug gcucggggca     1500

agagcgacuu cgccuggcuu uccagaaaaa gcgccgauaa gauuaccccc uggaacuucg     1560

acgagaucgu cgauaaagaa ucuuccgcug aggccuuuau caaucgcaug acaaacuacg     1620

accucuaucu gccgaaucag aaggugcugc cuaaacacag ccuccuguac gaaaaguuca     1680

ccguuuauaa cgagcuuacc aaagugaagu acaagacaga acaggggaaa acggcguuuu     1740

ucgaugcuaa uaugaagcag gagauuuuug acggagucuu caaaguguac cggaagguca     1800

ccaaagauaa gcugauggac uuucucgaga aggaguucga ugaauuuaga aucguggacc     1860

ugacaggccu ggacaaagaa aacaagguuu ucaaugccuc uuauggcacc uaccaugauc     1920

uuugcaaaau ucucgacaag gauuuucugg acaacagcaa aaaugagaag auccuggaag     1980

auauugugcu cacacugacc cuuuucgagg accgggagau gauccgcaag agacuggaaa     2040

acuauuccga ucuccugacg aaagagcaag ucaagaaacu ggaacggaga cacuacacag     2100

gguggggacg ccuuucugcc gagcucauuc auggcauccg gaacaaggaa agcagaaaaa     2160

ccauccugga cuaucugauu gaugacggaa auuccaaccg caauuuuaug cagcucauca     2220

acgaugacgc ucugagcuuc aaggaggaga uugccaaggc ucaggugauc ggggaaaccg     2280

auaaucuuaa ccagguugug ucugacauug ccggcucccc cgcgaucaaa aaggggaucc     2340

ugcaaagccu caaaauuguc gacgagcugg ugaagaucau gggccaccag ccugaaaaua     2400

uugugguuga gauggccaga gagaaccagu ucacaaauca gggacggcgc aacucucaac     2460

agagacugaa aggccuuacc gauuccauca aggaauuugg gagccagauu cucaaagagc     2520

aucccgucga aaauucccaa cugcagaacg accggcuguu ccucuacuau cugcagaaug     2580

gacgcgauau guacacaggc gaggaacuug acaucgauua ccugucucag uaugacaucg     2640

aucacauuau cccccaggcu uuuauuaagg acaacagcau cgauaauaga gugcucacgu     2700

ccucuaagga gaaccggggg aaaagcgacg augugccuuc caaggacguc gugcgcaaaa     2760

ugaaaagcua cuggucuaaa cugcuguccg ccaaacuuau uacccaaaga aaguucgaca     2820

accugacaaa agcugagcgg ggaggccuca ccgaugacga uaaggccggg uuuaucaaac     2880

gccagcuggu ugaaacaaga cagaucacca agcacguggc gcggauucuc gacgagagau     2940

ucaauacgga aaccgaugag aacaauaaaa agauccgcca agucaagauu gugacacuga     3000

aaagcaaccu uguuucuaau uuucggaagg aguucgaacu guauaaagug agagagauca     3060

acgacuacca ucacgcccau gaugcuuauc ucaaugccgu cauuggcaag gcucugcugg     3120

gaguguaccc gcagcuugaa cccgaguuug uuuauggcga cuacccucac uuccacgggc     3180

auaaagaaaa caaggccacc gccaagaaau uuuucuacuc caauaucaug aacuucuuua     3240

agaaagauga cgugcgcaca gauaagaaug gagagauuau cuggaaaaag gacgaacaca     3300

ucagcaacau uaagaaaguc cugucuuauc cccaggugaa uaucgugaag aaagucgagg     3360

agcagaccgg cggguucagc aaggaaucca uucucccuaa aggaaacagc gauaagcuga     3420

ucccccggaa aacgaagaag uuuuacuggg acacaaagaa guauggcggg uucgacucuc     3480

cgauuguugc uuacuccauc cucgugaucg cggauauuga gaaaggcaag agcaaaaagc     3540

ugaagaccgu gaaagcccuu gucggaguga caaucaugga aaagaugacc uuugagagag     3600

acccuguugc uuuccuggag cgcaaaggcu aucggaaugu gcaagaagag aacauuauca     3660

agcuccccaa auacucccug uuuaagcugg aaaacgggag aaagcgccuu cuggccucug     3720

cccgggagcu ccagaaagga aaugaaauug uccugccuaa ccaucucggc acccugcuuu     3780

aucacgcuaa gaauauccau aaaguggaug agcccaagca ccuggacuac guugauaaac     3840

acaaggacga guucaaggaa cugcucgaug uggucagcaa cuuuuccaaa aaguacacac     3900

uggccgaggg gaaucuugaa aagaucaagg agcuguaugc gcagaacaau ggagaagacc     3960

ucaaagagcu ggcuucuagc uucauuaacc uccugacguu uaccgccauc ggcgccccug     4020

cuacauucaa guuuuucgau aaaaauauug acagaaagcg guacaccucc acaaccgaga     4080

uccuuaacgc cacgcugauu caucaaucua ucacagggcu guaugaaacc cgcauugauc     4140

ucaauaagcu gggcggagac aaaagacccg cggcuaccaa gaaagccggc caggccaaga     4200

aaaagaagua aggacuagug aagggaacac ccaaauuuaa uucagccuua agcacaauua     4260

auuaagagug aaacguaauu guacaagcag uuggucaccc accauagggc augaucaaca     4320

ccgcaaccuu uccuuuuucc cccagugauu cugaaaaacc ccucuucccu ucagcuugcu     4380

uagauguucc aaauuuagua agcuuaaggc ggccuacaga agaaaaagaa aaaaaaggcc     4440

acaaaaguuc ccucucacuu ucaguaaaua aaauaaaagc agcaacagaa auaaagaaau     4500

aaaugaaauu caaaaugaaa uaaauauugu guugugcagc auuaaaaaau caauaaaaau     4560

uaaaaaugag caagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaaaaaa aagaauu                                         4647


<210>  4659
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4659
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccca gacaacaaac cugagcuaca uucucggacu ggaccuuggc aucgcguccg      180

uugggugggc ugugguggag auuaaugaaa acgaggaucc gaucggacug auugacgucg      240

gcgugcggau cuucgaacgc gccgagguuc ccaagacggg ggaaucucuc gcccugagca      300

gacggcuugc ucgcuccacc agacggcuga uccgcagacg ggcccaucgc cuccugcugg      360

cuaaaagauu ucucaagcgg gaggggauuc ugucuacaau cgaucuugag aagggccugc      420

cuaaucaagc cugggaacuc cgcguggcgg gccuggagag acggcuuagc gccauugaau      480

ggggagcugu ccugcuccac cugaucaaac aucgcggaua ucuguccaag cggaaaaacg      540

agucucagac caauaacaag gagcucggcg cccugcuuag cgggguggcu cagaaucacc      600

agcugcucca auccgacgau uacagaacac ccgccgaacu ggcgcuuaaa aaguucgcca      660

aggaggaagg acauauuaga aaccagcgcg gcgcuuauac ccacacauuu aaucggcugg      720

accuccuggc cgagcugaac cuccuguucg cucagcagca ccaauuuggg aauccucauu      780

gcaaagaaca cauccagcag uacaugaccg agcuucugau guggcagaag cccgcccuca      840

gcggagaggc cauucugaaa augcuuggca aguguacgca ugaaaagaac gaguucaaag      900

cugcgaaaca caccuauucu gccgaaagau uuguuuggcu gacaaagcuc aauaaccugc      960

gcauccugga ggauggcgcu gaacgggccc ucaaugagga ggaaagacaa cugcuuauca     1020

accacccuua cgagaagucc aaacugaccu augcccaggu gcgcaagcuc cuggggcuga     1080

gcgaacaggc uauuuucaaa caucuucggu acucuaagga gaacgccgaa uccgcgacau     1140

uuauggagcu caaggcuugg cacgccauca gaaaagcccu ggagaaucaa ggacugaagg     1200

acaccuggca ggaucucgcu aaaaagccgg accugcuuga ugaaauuggc acggccuuca     1260

gccuguacaa aacagacgag gauauccagc aguaucucac caacaagguc cccaauucug     1320

ugauuaacgc ucugcuggug ucccuuaauu uugacaaauu caucgaacuc agccugaagu     1380

cccugcgcaa gauccucccu cugauggagc aagggaaacg guacgaccag gccugcagag     1440

agauuuaugg acaucacuac ggcgaagcga accagaagac aucucagcuu cugcccgcca     1500

ucccugcuca agagauucgc aaucccgucg uucuccggac ccugagccag gccagaaaag     1560

ugaucaacgc uauuauccgg caguaugggu ccccggcccg cgugcauauc gaaaccggaa     1620

gagagcuggg caagagcuuu aaagaacgga gagagauuca gaagcaacag gaggauaauc     1680

gcacaaagcg ggaaucugcg guccagaaau ucaaggagcu uuucuccgac uuuagcucug     1740

aaccuaaaag caaggauauc cucaaauuca gacuguacga gcagcaacac ggcaaguguc     1800

uguauuccgg gaaagaaauu aacauccacc gccucaauga gaagggauac guggagauug     1860

accaugcccu gcccuuuucu agaacguggg augacagcuu caacaauaag guucuugugc     1920

uggcuuccga aaaccagaau aaaggcaacc agaccccuua cgaguggcuc caagggaaga     1980

ucaacagcga acgguggaaa aauuuugucg cccuggugcu gggaucucag ugcuccgcug     2040

ccaagaaaca gcgccuucuc acacagguua uugaugacaa caaguucauc gauagaaauc     2100

ugaacgacac ccgguauauc gcccgcuuuc ugagcaauua cauucaagag aaccuccugc     2160

uugugggcaa gaauaaaaag aacgucuuca cacccaaugg gcagaucacc gcucugcuca     2220

gaucucggug gggccugauu aaagcgcgcg aaaacaauaa cagacaccau gcccuggaug     2280

cuaucguggu cgccugugcc acgcccucca ugcagcagaa gauuacacgg uuuauccgcu     2340

ucaaagaggu gcacccuuau aagaucgaga auagauacga aaugguugac caagagagcg     2400

gagaaauuau cuccccgcac uuucccgagc cuugggcuua uuuccggcag gaggugaaca     2460

uuagagucuu ugacaaucau cccgauaccg ugcuuaagga aaugcugccu gaccgccccc     2520

aggccaacca ccaguucguu caaccucucu ucgugucucg ggcgccgacc agaaaaauga     2580

gcggccaggg gcauauggag acaaucaagu ccgcuaaacg ccuggccgaa ggaauuucug     2640

uccuccggau cccccugacc cagcuuaagc cuaaccugcu cgagaauaug gugaacaaag     2700

aaagagagcc cgcccuguac gcuggccuga aggcccgccu ugcggaguuu aaucaagauc     2760

cugcuaaggc cuucgccaca cccuuuuaua aacaaggggg acaacaggug aaggcuaucc     2820

ggguugaaca gguccagaaa agcggcgugc uggugagaga gaacaauggg gucgccgaca     2880

acgcuuccau ugugcgcacg gauguuuuca ucaagaauaa caaauuuuuc cucgugccga     2940

uuuacaccug gcaggucgcc aagggcaucc ugccuaauaa agcgauugug gcccacaaga     3000

acgaagacga gugggaagag auggaugagg gagcuaaguu uaaauucagc cucuuuccca     3060

augaccuggu ugaacuuaag accaaaaagg aguacuucuu uggcuauuac aucgggcugg     3120

aucgggccac aggaaacauc ucucucaaag aacaugacgg cgagauuucc aaggggaagg     3180

auggagugua uagagucggc gugaaacugg cucugagcuu cgaaaaguac cagguugacg     3240

agcuugggaa aaaucggcag aucugccgcc cccagcagag acagccugug cggaagcgcc     3300

cggccgccac caaaaaggcu ggccaagcga agaaaaagaa auaaggacua gugaagggaa     3360

cacccaaauu uaauucagcc uuaagcacaa uuaauuaaga gugaaacgua auuguacaag     3420

caguugguca cccaccauag ggcaugauca acaccgcaac cuuuccuuuu ucccccagug     3480

auucugaaaa accccucuuc ccuucagcuu gcuuagaugu uccaaauuua guaagcuuaa     3540

ggcggccuac agaagaaaaa gaaaaaaaag gccacaaaag uucccucuca cuuucaguaa     3600

auaaaauaaa agcagcaaca gaaauaaaga aauaaaugaa auucaaaaug aaauaaauau     3660

uguguugugc agcauuaaaa aaucaauaaa aauuaaaaau gagcaagauc uaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3780


<210>  4660
<211>  3858
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4660
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc ggcuuucaag ccgaacccca uuaauuacau ccugggacuc gacauuggca      180

ucgccagcgu ugggugggcc augguggaga uugaugaaga cgagaacccu aucugccuga      240

ucgaucuugg agugcggguc uuugaacgcg cugaggugcc caaaaccggc gacucccugg      300

ccauggcuag acggcucgcc cgcucuguua gacggcugac acgcagacgg gcgcaucgcc      360

uucugagagc ccggcgccuc cugaagagag aaggggugcu gcaggcugcc gauuucgacg      420

agaauggacu cauuaagagc cugccuaaca cgcccuggca acuucgggcu gccgcgcugg      480

aucgcaaacu caccccucug gagugguccg ccguccuucu gcaccucauc aagcauagag      540

gcuaucuguc ucagcggaaa aaugaaggcg agacagcuga caaggaacug ggggcccucc      600

ugaaaggagu ggcugauaac gcccacgccc uucagaccgg cgacuuuaga acaccggcug      660

agcuggcgcu caauaaguuc gagaaggaaa gcgggcauau ucgcaaccag cggggagauu      720

acucccacac cuuuucuaga aaagaccugc aagccgagcu uauccuccug uucgaaaagc      780

agaaagaguu uggcaauccc cacguuagcg ggggacugaa ggaaggcauu gagacacugc      840

ucaugaccca gcgcccugcu cuguccggcg augccgugca gaaaaugcuu gggcauugua      900

cguucgagcc cgccgaaccu aaggcugcca aaaacaccua uacagcggag cgguuuaucu      960

ggcugaccaa gcucaauaac cugcgcauuc uugaacaagg aagcgagaga ccccugacag     1020

acaccgaacg ggcuacgcuc auggacgagc cguacagaaa gucuaaacug acauaugccc     1080

aggcccgcaa gcugcucggc cuggaggaua ccgcuuucuu uaaagggcuu cgguacggaa     1140

aggacaaugc cgaggcuucc acacugaugg aaaugaaagc cuaucacgcg aucagcagag     1200

cccucgaaaa ggagggccug aaggauaaaa agucuccucu gaaccuuucc cccgaacucc     1260

aggacgagau cgggaccgcu uucagccugu uuaaaaccga ugaggacauu acaggacgcc     1320

ugaaggaucg gauccagccu gaaauucucg aggcccugcu uaaacauauc ucuuucgaca     1380

aguucgucca aauuucccug aaggcucuca gacggaucgu gccccugaug gaacagggca     1440

aacgcuacga ugaggccugc gcggagaucu acggcgacca cuaugggaag aaaaauacgg     1500

aagagaagau uuaccugccc ccuaucccgg ccgaugaaau uagaaacccc gugguccuuc     1560

gggcucucag ccaggccaga aaaguuauca acggaguggu gcgccgguau ggcuccccug     1620

cucgcauuca caucgagacc gccagagaag ucgggaaguc uuuuaaggac agaaaagaga     1680

ucgagaagcg gcaagaagag aaucgcaaag acagagaaaa ggccgcugcg aaauuccggg     1740

aguacuuucc caacuucgug ggagaaccua agagcaaaga uauucugaag cugcgccucu     1800

augagcagca gcauggcaag ugucuguacu ccgggaaaga gaucaaucuu ggcagacuga     1860

acgaaaaggg auauguugag auugaccacg cccuccccuu uagccggaca ugggaugacu     1920

cuuucaauaa caaagugcug guccugggcu ccgaaaauca gaacaagggg aaucaaaccc     1980

cguacgagua cuuuaacgga aaagauaaua gccgcgaaug gcaggaguuc aaggcuagag     2040

uggagacauc ucgguuuccu cgcagcaaga aacagagaau ccuucuccag aaguucgacg     2100

aagauggcuu uaaagagcgg aaccugaaug acaccagaua uguuaaccgc uuccugugcc     2160

aauuuguggc cgaucggaug agacucacgg ggaagggcaa aaagcgcguc uucgccucca     2220

auggacagau uacaaaccug cuucgggggu ucuggggccu gagaaaggug cgcgcugaaa     2280

acgaccggca ucacgcccuc gaugcggucg ugguugcuug uucuaccgug gccaugcagc     2340

agaaaaucac cagauuuguc cgcuacaagg agaugaaugc cuucgacgga aaaacaauug     2400

auaaggagac cggcgaagug cugcaucaga aaacacacuu uccccagccu ugggaguucu     2460

uugcucagga aguuaugauc cggguguucg ggaagcccga cggaaagccu gaguuugaag     2520

aggccgacac gcccgagaaa cugagaaccc uucucgcgga aaagcugagc ucccggccgg     2580

aggcugucca cgaauaugug acaccucugu ucgugagccg cgcccccaac agaaaaaugu     2640

cuggccaggg gcauauggag accguuaagu ccgccaaacg gcucgaugaa ggagucagcg     2700

ugcugcgcgu gccccuuaca caacugaagc ucaaagaccu ggagaagaug gucaauagag     2760

agcgggaacc uaagcuguac gaggcucuua aagccagacu ggaagcucac aaggaugacc     2820

cggccaaagc guuugccgag cccuucuaua aguacgauaa agcuggcaac cgcacccaac     2880

aggugaaggc cguucgggug gaacaagucc agaagacggg gguguggguu agaaaucaua     2940

acggcaucgc ugacaaugcc accauggugc gcgucgaugu guuugagaaa ggagacaagu     3000

auuaccucgu uccuauuuac ucuuggcaag uggccaaagg cauccugccc gaucgggcug     3060

ucgugcaggg gaaggacgag gaagauuggc agcucauuga cgauuccuuc aacuuuaaau     3120

ucagccugca cccuaaugac cuuguggagg ucaucacaaa gaaggcgaga auguucggau     3180

auuuugccuc cugccaccgc ggcaccggga acauuaauau ccggauccau gaccuggauc     3240

acaaaauugg aaagaacggc auccucgaag ggauuggcgu uaaaacagcu cugucuuucc     3300

agaaguacca gaucgacgag cugggaaaag agauuagacc cugucgccuu aagaagagac     3360

cucccgugcg gaaacgcccg gccgccacca agaaagcugg ccaggccaag aaaaagaagu     3420

aaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu aauuaagagu     3480

gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac accgcaaccu     3540

uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc uuagauguuc     3600

caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc cacaaaaguu     3660

cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa uaaaugaaau     3720

ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa uuaaaaauga     3780

gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaagaauu                                                   3858


<210>  4661
<211>  3864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4661
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa guaccauguu ggaauugacg ugggcaccuu cagcgugggg cuggcggcua      180

ucgaggucga ugacgccgga augccgauua aaacacucuc ccuggugucu cacauccaug      240

auagcggccu ugaccccgau gaaauuaagu ccgccguuac gcggcuggcu ucuagcggga      300

ucgcccgcag aacccggcgc cucuauagac ggaagcgcag acggcugcag caacuugaca      360

aauuuaucca gcgccaggga uggccuguga uugagcugga agauuacucc gacccccucu      420

auccuuggaa ggucagagcu gagcuggccg cgucuuacau cgccgaugaa aaagagcggg      480

gcgagaagcu gagcguggcu cuccgccaca uugccagaca ucggggcugg cgcaaccccu      540

augcuaaagu uuccagccug uaccuuccug acgggccguc ugaugccuuc aaggcgauca      600

gagaagagau uaagcgggcc uccggacagc ccgugccuga aacagcuacc gucggccaaa      660

uggugacacu gugcgagcuc gggacccuga aacuuagagg agagggcggg gugcugagcg      720

cccgccucca gcagucugac uaugcucggg aaauccagga gauuuguaga augcaagaaa      780

ucggacagga gcuguaccgc aagaucauug augucguuuu ugccgccgaa ucccccaaag      840

gcagcgcuuc uucccgggug ggcaaggacc cucugcagcc cgggaaaaau agagcgcuca      900

aggccagcga cgcuuuccag cgcuaccgga ucgccgcccu gauuggaaac cuuagagugc      960

gcgucgaugg cgagaaacgg auccuguccg uggaggaaaa gaaucucguu uuugaccacc     1020

uggugaaccu uacaccgaag aaagagccug aaugggucac cauugcugag auccugggga     1080

ucgauagagg acaacucauu ggcacggcca ccaugacaga cgauggggaa cgcgcgggag     1140

cucggccccc uacccacgac acaaauagau cuaucgugaa cagccggauu gcuccccugg     1200

uugauuggug gaagaccgcc uccgcccugg agcagcaugc cauggugaaa gcucucagca     1260

augccgaggu cgacgauuuc gacucucccg aaggcgcgaa ggugcaggcc uuuuucgcug     1320

aucuggacga cgauguccac gccaaacuug acucccugca ucucccugug ggccgcgcug     1380

ccuauagcga ggauacgcug guuagacuua cacggagaau gcugucugac gggguggauc     1440

ucuacaccgc gcgccugcaa gaauuuggaa ucgagccgag cuggacaccc ccuacacccc     1500

ggauuggcga accugucggg aaccccgccg uggacagagu ucugaagacc gugucccgcu     1560

ggcucgaguc ugcuaccaag acguggggag ccccggagag agucaucauc gaacacgugc     1620

gggagggcuu cgugaccgaa aaacgcgcua gagagaugga uggggacaug cggcgcagag     1680

ccgcccggaa ugcuaagcug uuucaggaga ugcaggaaaa acuuaacguu cagggcaagc     1740

cuagccgcgc ggaucugugg agauaucaau ccguccagcg gcagaauugc cagugugccu     1800

acugcggaag ccccauuaca uucucuaacu ccgagaugga ccacaucgug ccucgcgcug     1860

gccaagggag caccaauaca agagaaaacc ucguggccgu cugucaucgg ugcaaucagu     1920

cuaaaggaaa cacccccuuu gccauuuggg cuaagaacac guccaucgag ggcgugagcg     1980

uuaaggaagc cguggagaga acacgccacu gggucaccga uaccgggaug cgguccacag     2040

acuucaaaaa guucaccaaa gcggugguug agagauuuca gcgcgcuaca auggaugaag     2100

agauugacgc ccggucuaug gaaagcgugg ccuggauggc uaaugagcug agaucccgcg     2160

ucgcccagca uuucgcgucu cacggaacga ccgugcgggu uuauagaggc agccugacag     2220

cugaagcccg ccgggccucc gggaucagcg gcaagcugaa auuuuucgac ggagugggca     2280

agucuagacu cgaucggcgc caucacgcua uugacgccgc ugucaucgcc uuuaccuccg     2340

auuacguggc ggagacacug gccgugagaa gcaaccugaa gcaaucucag gcucaccggc     2400

aggaggcccc ucaguggcgc gaauuuaccg ggaaagacgc ugagcauaga gccgccuggc     2460

gggucuggug ucaaaagaug gaaaaacucu ccgcucugcu uacggaggau cugagagacg     2520

aucgcguugu ggugaugagc aauguccggc ucagacuggg aaacggcucu gcgcacaagg     2580

aaaccaucgg gaaacugagc aaggugaaac uuuccagcca gcucucuguu uccgacauug     2640

auaaggccag cuccgaggcu cuguggugcg cccugacacg cgagcccgga uuugacccga     2700

aggaaggccu cccugccaau cccgagcggc auaucagagu gaacgggacc cacgucuaug     2760

cuggcgauaa uauuggacug uuccccgugu cugccggcag caucgcgcuu cgcgggggau     2820

acgcugaacu gggcuccucu uuucaccaug cccggguuua caaaauuaca agcgggaaga     2880

aaccugccuu cgcuaugcuc agaguguaua ccaucgaccu gcugccguac cgcaaccagg     2940

aucuuuucuc cgucgagcuc aagccccaaa cgaugucuau gagacaggcc gagaaaaagc     3000

ugcgggacgc gcuggcuaca ggaaaugccg aauaucucgg cuggcuggug guugacgaug     3060

agcuuguggu cgacaccagc aagaucgcca cagaucaggu gaaagcugug gaagccgagc     3120

uggggaccau ucgcagaugg cgggucgacg gcuucuuuuc cccuagcaag cuccgccuga     3180

gaccccugca gaugucuaaa gaaggaauca agaaagaguc cgcuccugag cuuagcaaga     3240

uuaucgaucg gcccggcugg cucccugccg uuaacaagcu guucucugac gggaauguga     3300

ccguggucag acgcgauucc cugggacggg ugagacucga aagcacagcg caccuucccg     3360

uuacguggaa agugcaaaag cgcccggccg cuaccaaaaa ggccggccag gcuaaaaaga     3420

agaaauaagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc acaauuaauu     3480

aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug aucaacaccg     3540

caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca gcuugcuuag     3600

auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa aaaggccaca     3660

aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua aagaaauaaa     3720

ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa uaaaaauuaa     3780

aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaag aauu                                            3864


<210>  4662
<211>  3564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4662
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc gcggauucug gcuuucgaca ucggaauuag cuccaucggc ugggccuuuu      180

cugagaacga ugaacucaag gacugcgggg uucgcauuuu caccaaagug gagaauccga      240

agacaggaga aagccuggcc cuucccagac ggcuggcucg cuccgccaga aagcggcucg      300

cucgcagaaa agcccggcug aaccaucuua agcaccugau cgcgaaugag uuuaaacuca      360

acuacgaaga uuaucagucu uucgacgaga gccuggccaa ggcuuacaaa ggcucccuga      420

ucucuccuua ugagcuccgc uuuagagccc ugaaugaacu ucugagcaag caagauuucg      480

cucgggugau ucuccauauc gccaagcgca gaggguacga cgauauuaaa aacuccgacg      540

auaaggagaa aggagcgauc cugaaggcca uuaaacagaa ugaagagaaa cuugcuaacu      600

aucagagcgu cggcgaguac cuguauaaag aauacuuuca gaaguucaag gaaaauucua      660

aagaauuuac gaacgugcgg aauaagaaag aguccuacga acgcuguauc gcccagagcu      720

uccucaagga cgagcugaaa cugauuuuua agaagcaaag agaguucggc uuuucuuucu      780

ccaaaaaguu cgaagaggaa guucucagcg uggcuuuuua uaaacgggcc cugaaggauu      840

ucucucaccu ugucgggaac ugcuccuuuu ucaccgacga gaaaagagcc cccaagaaua      900

gcccucuggc uuuuauguuc guggcgcuca cacgcaucau caaccugcuu aauaaccuga      960

agaacaccga aggcauucuc uacacaaaag augaccugaa ugcccugcuc aacgagguuc     1020

ugaagaaugg aacccuuaca uauaaacaga ccaagaaacu gcucgggcug uccgacgauu     1080

acgaguuuaa gggagaaaag ggcacguauu ucaucgaguu uaaaaaguac aaagaguuca     1140

uuaaggcucu uggggagcau aaccugucuc aggacgaucu caaugaaauc gccaaagaca     1200

uuacccugau caaggaugag aucaaacuga agaaggcccu cgcuaaauau gaccugaacc     1260

aaaaucagau ugauagccuu uccaagcugg aguuuaaaga ccaccucaac aucagcuuca     1320

aggcccugaa acuggugaca ccccuuaugc ucgaaggaaa gaaguacgau gaggcgugua     1380

augaacugaa ccugaaaguc gcuauuaaug aggacaagaa agauuuucuc ccugccuuca     1440

acgagaccua cuauaaggac gaagugacaa auccgguggu ccugcgggcc aucaaagagu     1500

acagaaaggu ucuuaacgcu cugcucaaga aauauggcaa ggugcacaaa auuaauaucg     1560

aacuggcccg cgaggugggc aagaaccauu cucagcgggc uaaaaucgaa aaggagcaga     1620

acgagaauua caaggccaaa aaggacgcgg aacuggagug cgaaaaacuu gggcucaaga     1680

uuaacuccaa aaauauccug aagcugagac ucuucaaaga gcaaaaggaa uuuugugccu     1740

auagcggaga gaagauuaaa aucucugauc ugcaggacga gaagaugcuu gaaauugauc     1800

acaucuaccc cuauagccgc uccuucgacg auucuuacau gaacaaaguc cugguguuua     1860

ccaagcagaa ucaagagaaa cucaaccaga cgccuuucga ggcuuuuggc aaugacagcg     1920

ccaaguggca gaagauugaa guucuggcua aaaaccugcc cacaaagaaa cagaagcgga     1980

uccuugauaa aaauuacaag gacaaggaac aaaagaacuu caaggauaga aaucucaacg     2040

acacccgcua uaucgcccgg cuggugcuga auuacacaaa agauuaucuc gacuuucugc     2100

cucuuuccga ugacgagaac accaagcuga augacaccca gaaagggagc aagguccaug     2160

uggaggcgaa gucuggaaug cucacauccg cccugagaca cacguggggc uucagcgcua     2220

aagaucgcaa caaccaucug caccacgcca uugacgcugu uaucauugcc uacgccaaua     2280

acucuaucgu gaaggcuuuu uccgauuuca aaaaggaaca ggagagcaau uccgcggaac     2340

uuuaugccaa aaagauuucu gagcucgacu acaagaacaa acggaaguuu uucgagcccu     2400

uuagcggguu cagacagaaa guccuggaua agaucgacga aaucuucgug uccaaaccgg     2460

agcggaagaa accuucugga gcucugcaug aagagaccuu ucgcaaggaa gaggaguucu     2520

aucaaagcua cggcggcaag gaaggggucc ucaaagcccu ggagcuugga aagauuagaa     2580

aagugaaugg caagaucguu aaaaacgggg auauguuucg gguggacauu uucaagcaca     2640

agaaaacaaa uaaguuuuac gccgucccca ucuauaccau ggauuucgcu cugaaagugc     2700

ucccuaacaa ggccguugcg agauccaaaa agggagaaau uaaggacugg auccugaugg     2760

augagaauua cgaauuuugc uucagccugu auaaagacuc ucuuauccug auucagacaa     2820

aggauaugca ggagcccgag uuuguguacu auaacgcuuu caccuccagc acggucucuc     2880

ucaucguguc caaacaugac aauaaguuug aaacacugag caaaaaccag aagauucucu     2940

ucaagaaugc caacgagaaa gaagugaucg ccaagucuau uggcauccaa aaucugaaag     3000

uuuucgagaa guacaucguc agcgcucuug gggaagugac caaagccgag uuucgccagc     3060

gggaggacuu caagaaaaag agacccgcgg cuaccaagaa agccggccag gccaagaaaa     3120

agaaauaagg acuagugaag ggaacaccca aauuuaauuc agccuuaagc acaauuaauu     3180

aagagugaaa cguaauugua caagcaguug gucacccacc auagggcaug aucaacaccg     3240

caaccuuucc uuuuuccccc agugauucug aaaaaccccu cuucccuuca gcuugcuuag     3300

auguuccaaa uuuaguaagc uuaaggcggc cuacagaaga aaaagaaaaa aaaggccaca     3360

aaaguucccu cucacuuuca guaaauaaaa uaaaagcagc aacagaaaua aagaaauaaa     3420

ugaaauucaa aaugaaauaa auauuguguu gugcagcauu aaaaaaucaa uaaaaauuaa     3480

aaaugagcaa gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaag aauu                                            3564


<210>  4663
<211>  3975
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4663
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccag cgaccugguu cucggacugg auauuggcau cggguccgug ggagugggca      180

uucuuaacaa ggucaccggg gagaucauuc auaaaaauuc ucggaucuuc ccggcggcuc      240

aggccgaaaa caaucuggug cgcagaacaa accggcaagg acgcagacuc gcccggcgca      300

agaagcacag acggguucgc cugaauagac uuuuugagga aagcggccug aucacggacu      360

ucaccaaaau uuccaucaac cucaaucccu accagcugcg ggugaagggc cugacagaug      420

agcucucuaa cgaggagcug uuuauugcuc uuaaaaauau ggucaagcau cgcgggauca      480

gcuaucugga cgaugccucc gacgauggaa acucuagcgu gggcgacuac gcucagauug      540

uuaaagaaaa uuccaagcag cucgaaacca agacaccugg gcaaauccag cuggagagau      600

aucagaccua cggacagcuu cggggcgauu ucacagugga aaaagacggg aagaaacacc      660

gccugauuaa cgucuuuccc accagcgccu auagaucuga ggcgcuccgg auccugcaaa      720

cgcagcagga guucaauccu cagaucaccg augaauuuau uaacagauac cuggagaucc      780

ucacaggaaa gcgcaaauau uaccauggcc ccggcaauga aaagucccgg accgacuacg      840

ggagauaucg cacaagcgga gagacccugg auaacauuuu cggcauccuu auugggaaau      900

gcacguuuua cccugacgag uuccgggccg cuaaggccuc uuauacagcu caagaauuua      960

accugcucaa ugaccugaac aaucuuaccg ugccgacaga gaccaagaaa cuguccaagg     1020

aacagaaaaa ccagaucauc aauuacguga agaacgagaa agccauggga cccgcgaaac     1080

ucuucaagua uauugccaaa cugcugagcu gugaugucgc ugacaucaag ggcuacagaa     1140

uugauaaauc ugggaaggcc gaaauccaca ccuuugaggc uuaucgcaaa augaagacac     1200

ucgaaacgcu ggacauugag caaauggauc gggagacccu ugacaagcug gccuacguuc     1260

ucacacugaa uaccgaaaga gagggaaucc aggaagcccu ugagcacgag uucgcugaug     1320

gcuccuucag ccagaaacag guggacgaac uggugcaauu ucgcaaggcg aacuccucua     1380

ucuucggcaa aggguggcau aauuuuagcg ucaagcucau gauggagcug auuccugaac     1440

uguacgagac auccgaggaa cagaugacca uccucacgcg gcugggaaaa cagaagacaa     1500

ccucuucuuc caacaagacc aaauauauug augagaaacu ucugacagaa gagaucuaca     1560

aucccguggu ugccaagagc gugagacagg cuauuaagau cgucaacgcc gccauuaaag     1620

aauauggcga cuucgauaau aucgugaucg agauggcucg cgaaaccaac gaggacgacg     1680

agaagaaagc cauucaaaag auccagaagg cgaauaaaga ugaaaaggac gcugccaugc     1740

ucaaagccgc uaaccaguac aacgggaagg ccgagcugcc ucacucuguu uuucauggac     1800

acaaacagcu ggcuacaaag auucggcuuu ggcaccaaca gggcgaaaga ugccucuaua     1860

cggggaagac caucagcauu caugaucuga ucaauaacuc caaucaguuc gagguggacc     1920

acauccugcc ccucucuauu acauuugaug acagccuggc caacaaaguc cuuguguacg     1980

cgaccgccaa ucaggaaaag ggccaacgga caccguauca ggcucuggau uccauggacg     2040

augccuggag cuuccgcgag cucaaggcuu uugucagaga gucuaagacc cuguccaaca     2100

aaaagaagga auaccugcuu acggaggaag acaucagcaa auucgaugug cggaagaaau     2160

uuauugagag aaaucucguu gacacccgcu acgccucucg ggugguccug aacgcgcugc     2220

aggaacauuu cagagcccac aagaucgaua caaaaguguc cguugugcgc ggacaauuua     2280

ccagccagcu cagacggcau uggggcauug agaagacacg cgacaccuau caccaccaug     2340

cugucgacgc ccugaucauc gcugccuccu cucagcuuaa ucuguggaag aaacagaaga     2400

acacgcucgu gagcuacucc gaggaucaac ugcuggacau ugaaacaggg gagcuuaucu     2460

cugaugacga auauaaagag agcguguuca aggccccuua ccagcacuuc guugauaccc     2520

ucaaauccaa ggaguuugaa gacagcauuc uguucucuua ucaggucgau uccaaauuua     2580

auagaaagau cagcgacgcu acaauuuacg cgacccggca ggccaaggug ggaaaagaua     2640

aggcugacga gaccuaugug cugggcaaaa ucaaggauau cuacacacaa gacggguacg     2700

augccuucau gaaaauuuau aagaaggaca aaucuaaguu ucucauguac cgccaugacc     2760

cccagacguu cgaaaaaguc aucgagccua uucuggaaaa cuaucccaau aagcagauca     2820

acgagaaagg aaaggaggug cccuguaauc cuuuucuuaa guacaaagag gaacacggcu     2880

auauuagaaa guacuccaaa aaggggaacg gcccggaaau caaaagccug aaguauuacg     2940

auucuaagcu cggaaaccac auugacauca cccccaaaga uagcaauaac aagguugugc     3000

ugcaguccgu cagcccuugg cgggccgacg uguacuucaa uaaaacaacc ggcaaguaug     3060

agauccuggg gcuuaaauac gcugaucucc aauuugaaaa gggaacaggc accuauaaga     3120

uuucucagga gaaauacaac gacaucaaga aaaaggaggg gguugauucc gacagcgagu     3180

ucaaauuuac gcuguauaag aaugaucugc uccuggugaa agacacagaa accaaggaac     3240

agcaacuuuu ccgcuuucug uccagaacca ugcccaagca gaaacauuac gucgagcuca     3300

agccuuauga uaaacagaag uucgaaggag gcgaggcccu gauuaaagug cuggggaacg     3360

uugcgaauag cggccagugc aagaagggac uuggcaaaag caacaucucc auuuacaagg     3420

ugcggacaga cguccugggg aaucaacaca ucaucaaaaa cgagggagac aagcccaaac     3480

ucgauuucaa gcgcccggcu gccaccaaga aagccggcca ggcuaagaaa aagaaauaag     3540

gacuagugaa gggaacaccc aaauuuaauu cagccuuaag cacaauuaau uaagagugaa     3600

acguaauugu acaagcaguu ggucacccac cauagggcau gaucaacacc gcaaccuuuc     3660

cuuuuucccc cagugauucu gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa     3720

auuuaguaag cuuaaggcgg ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc     3780

ucucacuuuc aguaaauaaa auaaaagcag caacagaaau aaagaaauaa augaaauuca     3840

aaaugaaaua aauauugugu ugugcagcau uaaaaaauca auaaaaauua aaaaugagca     3900

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3960

aaaaaaaaaa gaauu                                                      3975


<210>  4664
<211>  4533
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4664
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccac ccaguucgag ggauuuacaa accuguacca aguuagcaag acgcuccggu      180

ucgaacugau uccgcagggc aaaacccuua agcauaucca ggagcagggg uuuauugaag      240

aggacaaggc gcgcaaugau cacuauaaag aacugaagcc caucauugac agaaucuaca      300

aaacauaugc ugaucaaugc cuccagcugg ugcagcuuga cugggagaac cuguccgccg      360

ccaucgauuc uuaccggaag gagaaaaccg aagagacacg caaugcucuc auugaagaac      420

aggccaccua uagaaacgcu auccaugacu acuucauugg acggacagau aaucugaccg      480

acgccaucaa caagcgccac gcggagauuu auaagggccu guuuaaagcc gaacucuuca      540

augggaaggu gcugaaacaa cuuggaacgg ucaccacaac cgagcaugaa aacgcucugc      600

ucagauccuu ugauaaguuc acaaccuacu uuagcggcuu cuacgagaau cggaaaaacg      660

uguuuucugc cgaagacauc agcacggcua uuccucaccg caucguucag gauaauuucc      720

ccaaguuuaa agagaacugu cacaucuuca caagacugau uaccgccgug ccuucccuuc      780

gggagcauuu cgaaaauguc aagaaggcga ucggcauuuu ugugucuaca agcaucgagg      840

aaguuuucuc cuuucccuuc uauaaccagc ugcucaccca gacccaaauu gaccuguaca      900

aucagcugcu cgggggaauc agccgcgagg ccggcacaga gaaaaucaag gggcugaacg      960

aggugcuuaa ccuggcuauu cagaaaaaug augagacggc ccacaucauu gcuucucucc     1020

cucauagauu uaucccgcug uucaagcaaa uucuuuccga ccggaacacc cugagcuuua     1080

uccucgaaga guucaaaucu gacgaagagg ucauccaguc cuuuugcaag uauaagacac     1140

ugcugcgcaa ugaaaacgug cucgagaccg ccgaggcccu guucaaugaa cuuaacagca     1200

uugaucugac acacaucuuu auuucucaca aaaagcucga gaccaucucc agcgcucugu     1260

gugaccauug ggauacgcuu agaaaugcgc uguacgaacg gagaauuucc gagcucacag     1320

gaaaaaucac caagucugcc aaagagaagg ugcagcgcag ccugaagcac gaagacauua     1380

accugcagga gaucaucucc gcugccggca aagaacucag cgaggccuuc aagcagaaaa     1440

ccucugaaau ucugucccau gcucacgccg cgcuugauca accccugccu acaacccuca     1500

agaaacagga ggagaaggaa auccuuaaga gccagcugga cucucuucuc gggcuguauc     1560

aucugcucga uugguuugcu gucgacgaga gcaaugaagu ugaucccgag uucuccgccc     1620

ggcugacagg aauuaaacuu gaaauggagc cuucucugag cuucuacaac aaggccagaa     1680

auuaugcuac gaaaaagccc uacuccgugg agaaauuuaa gcucaacuuc caaaugccga     1740

cccuggccag cggcugggac gugaauaaag aaaagaacaa uggcgcuauc cuguuuguca     1800

agaacgggcu uuacuaucuc ggaauuaugc cuaaacagaa gggccgcuac aaagcccugu     1860

cuuucgagcc cacagaaaag accuccgagg gguuugauaa aauguauuac gacuauuucc     1920

cugacgcggc caagaugauc cccaagugca gcacacagcu gaaagcugug accgcccacu     1980

uucagacgca caccacaccc auccuccugu cuaacaauuu cauugaaccu cuugagauca     2040

ccaaggagau uuacgaucug aacaauccgg aaaaagagcc caagaaauuu caaacagcuu     2100

augccaagaa gaccggagac cagaaaggcu accgggaagc gcucuguaag uggaucgauu     2160

ucacgagaga cuuucugucc aaauacacaa agaccacaag cauugaucug uccucucuuc     2220

gcccuagcuc ccaguauaaa gaccucgggg aguacuaugc cgagcugaac ccccugcucu     2280

accauaucuc uuuccagcgg aucgcugaaa aggagauuau ggaugccguu gaaaccggaa     2340

agcuguaucu uuuucaaauc uacaauaaag acuucgcuaa gggccaccau ggcaaaccua     2400

accugcacac ccucuauugg acagggcugu ucagccccga gaaucuggcc aagacgucca     2460

uuaaacuuaa cggacaggcc gaacucuuuu acagaccuaa gagccgcaug aaacggaugg     2520

cucacagacu gggcgagaag augcugaaua agaaacucaa ggaucagaaa accccgaucc     2580

ccgacacacu guaccaagag cuuuaugauu acgugaacca ucgccugucu cacgaccucu     2640

ccgaugaagc gcgggcccug cugccuaaug ucauuaccaa ggaggugagc caugaaauca     2700

ucaaagacag acgguucaca ucugacaagu uuuucuuuca cguucccauu acccuuaacu     2760

aucaggcugc caauuccccu agcaaguuca accagcgcgu gaaugccuac cugaaagagc     2820

aucccgaaac gccgaucauu gggaucgaua gaggagagcg gaaccucauu uauaucacag     2880

ucauugacuc uaccggcaag auccuggagc agagaagccu caacaccauc caacaguuug     2940

auuaccagaa aaagcuggac aaucgcgaaa aagagcgggu ggcugccaga caggcguggu     3000

ccgucguggg gacaauuaag gaucuuaagc aaggcuaucu gagccagguu auccacgaaa     3060

uuguggaccu caugauccac uaccaggcug ucgugguucu ggagaaccug aauuucggau     3120

uuaaaucuaa gcgcaccggc auugccgaaa aagccgugua ccagcaauuc gagaagaugc     3180

uuaucgauaa acugaacugc cucguccuga aggacuaucc ugcugagaag guugggggag     3240

ugcucaaucc cuaccagcug acagaucagu uuacguccuu cgccaaaaug ggcacccaaa     3300

gcggguuccu guuuuaugug cccgcgccuu acacauccaa gaucgacccg cugaccggau     3360

ucgucgaucc cuuugugugg aaaacaauua agaaccauga aucuagaaaa cacuuccucg     3420

agggcuuuga cuuccugcau uaugauguga agaccgggga cuuuauccug cacuucaaga     3480

ugaaucggaa ccuuagcuuu cagcgcggcc ugccuggauu caugcccgcu ugggacauug     3540

ucuuugagaa aaaugaaacg caguucgaug ccaagggcac cccuuuuauc gccgggaaaa     3600

gaauugugcc cguuaucgag aaccaucggu ucacaggacg cuacagagac cucuauccug     3660

cuaaugaacu gaucgcccuc cuggaggaaa agggcauugu guuccgggau ggguccaaca     3720

uccuucccaa acugcuggag aaugacgauu cucacgcuau ugacaccaug gucgcccuca     3780

uccgcagcgu gcugcagaug agaaacucca augcggccac aggagaagau uacauuaacu     3840

cuccgguucg ggaccuuaac ggcguguguu uugauagccg cuuccaaaau ccugaguggc     3900

ccauggacgc ugaugccaac ggggcuuacc acaucgcccu gaagggccag cuccugcuca     3960

aucaucugaa agaguccaag gaccuuaagc ugcagaacgg aauuagcaau caggacuggc     4020

uggccuauau ccaagaacuc agaaacaaac ggccugcugc gaccaagaaa gccggccagg     4080

cuaagaaaaa gaaguaagga cuagugaagg gaacacccaa auuuaauuca gccuuaagca     4140

caauuaauua agagugaaac guaauuguac aagcaguugg ucacccacca uagggcauga     4200

ucaacaccgc aaccuuuccu uuuuccccca gugauucuga aaaaccccuc uucccuucag     4260

cuugcuuaga uguuccaaau uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa     4320

aaggccacaa aaguucccuc ucacuuucag uaaauaaaau aaaagcagca acagaaauaa     4380

agaaauaaau gaaauucaaa augaaauaaa uauuguguug ugcagcauua aaaaaucaau     4440

aaaaauuaaa aaugagcaag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  4533


<210>  4665
<211>  4512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4665
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccag cauuuaccag gaguucguua acaaguauuc ccugucuaaa acccuccggu      180

uugaacugau cccgcaagga aagacacuug agaauauuaa ggcgcgcggc cugauccucg      240

acgaugaaaa gagagcuaag gacuacaaaa aggccaaaca gauuaucgau aaguaucauc      300

aguucuuuau cgaggaaauu cugagcuccg ugugcaucuc ugaggaccuu cugcagaacu      360

acagcgaugu guauuucaag cucaaaaagu ccgacgauga caaucugcaa aaagauuuua      420

agucugccaa agacacgauu aagaaacaga ucagcgagua cauuaaggau uccgaaaagu      480

ucaaaaaccu guuuaaucag aaccucaucg acgcuaagaa agggcaggag agcgaucuga      540

uucuuuggcu gaagcaaucu aaagacaaug gaaucgaacu cuucaaggcc aacuccgaca      600

ucaccgauau ugacgaggcu cuggagauca uuaagagcuu uaaaggcugg acaaccuauu      660

ucaagggguu ucacgaaaau cggaaaaacg ucuacucuuc caaugauauc cccacaagca      720

uuaucuaccg caucguggac gauaaccuuc cuaaguuccu ggagaauaaa gccaaguaug      780

aaucucucaa ggacaaagcg cccgaggcca uuaacuacga acagaucaag aaagaucugg      840

cugaggagcu gaccuuugac auugauuaua agacauccga aguuaaucag agaguguuca      900

gccucgacga ggucuucgaa aucgccaacu uuaacaauua ccugaaccag uccggaauua      960

ccaaauucaa uacgaucauc ggcggcaagu uugugaacgg ggagaauacc aagcggaaag     1020

gaauuaacga auauaucaau cuuuacucuc aacagauuaa cgauaagaca cugaaaaagu     1080

auaaaaugag cguucucuuc aagcagaucc uguccgacac cgagagcaaa ucuuuuguga     1140

uugacaaacu ugaggaugac uccgaugucg ugacaaccau gcagagcuuc uacgaacaaa     1200

ucgcugccuu uaagacggug gaggaaaaau cuauuaagga gacacugagc cuccuguucg     1260

acgaucugaa agcgcagaag cucgaccugu ccaaaaucua cuuuaagaau gauaagucuc     1320

uuaccgaccu gagccagcaa gucuucgaug acuauuccgu uaucggcaca gccgugcucg     1380

aauacauuac ccagcagauc gcuccuaaaa accuggauaa ucccagcaag aaagagcagg     1440

agcuuauugc caagaaaacc gaaaaggcua aguaucuguc ucucgagaca aucaaacugg     1500

cccuggaaga guuuaacaag caucgcgaca uugauaaaca auguagauuc gaggaaaucc     1560

ucgccaauuu ugcugcgauc ccuaugauuu ucgacgagau cgcccagaac aaggacaauc     1620

uggcucagau uuccaucaaa uaccagaacc aagggaagaa ggaucuucug caggccagcg     1680

ccgaagacga ugugaaagcu auuaaggacc uccuggauca gacgaacaau cugcuucaca     1740

aacucaagau cuuccauauc ucucaguccg aggacaaagc caacauucug gauaaggacg     1800

aacacuuuua ucuggucuuc gaggagugcu acuuugaacu cgcgaauauc gugccgcugu     1860

auaacaaaau ucggaauuac aucacccaaa agcccuacag cgaugagaag uucaaacuua     1920

acuuugaaaa uuccacacug gcuaacggau gggacaagaa uaaagagccu gauaacaccg     1980

ccauucucuu caucaaggac gauaaauauu accugggcgu uaugaauaag aagaacaaua     2040

aaaucuuuga cgacaaggcc auuaaagaaa acaaggggga gggauauaaa aagaucgugu     2100

acaagcugcu ucccggcgcu aauaaaaugc ucccuaaggu cuucuuuucu gccaaaagca     2160

uuaaguucua uaaccccucc gaggauaucc ugcgcauuag aaaccacucu acacauacca     2220

aaaauggcag cccgcagaag ggguacgaaa aguuugaguu caacaucgaa gacugucgga     2280

aauuuauuga uuucuauaag caguccauca gcaaacaccc ugaguggaag gacuucggau     2340

uucgcuucuc ugauacgcaa agauacaauu ccaucgacga auuuuaccgg gagguggaga     2400

accagggcua uaaacugaca uucgaaaaua uuagcgaguc uuacaucgau uccguuguga     2460

accaggggaa gcucuaucug uuucagauuu acaauaagga cuucagcgcu uauucuaaag     2520

gacgccccaa ccuucauacc cuguacugga aggcccucuu ugaugaaaga aaucugcaag     2580

acgucgugua uaaacugaac ggcgaggcgg agcuuuucua ccggaagcag agcaucccua     2640

aaaagauuac ccaccccgcc aaagaagcua ucgccaauaa gaacaaggau aaucccaaaa     2700

aggaguccgu cuuugaauac gaccucauca aagacaagag auucacagag gauaaauuuu     2760

ucuuccacug cccuauuacc aucaacuuua agagcucugg ggcuaauaag uucaacgacg     2820

aaauuaaccu gcugcucaaa gagaaggcca augaugugca uauccugucc auugaccgcg     2880

gagagcggca ccuugcguau uacacacugg uugauggcaa aggcaacauc aucaagcagg     2940

acacguuuaa uauuaucggg aacgauagaa ugaaaaccaa uuaucaugac aagcucgccg     3000

cuauugaaaa ggaucgcgac agcgcccgga aagauuggaa gaaaaucaac aauauuaagg     3060

agaugaaaga aggauaccug ucccaggugg uccacgagau cgcuaagcug gugauugaau     3120

auaacgccau cguuguguuc gaggaccuua auuuuggcuu caagagaggg cgcuuuaaag     3180

ucgagaagca aguguaccag aaacucgaaa agaugcugau cgagaaacug aacuaucucg     3240

uguucaagga uaaugaauuu gacaaaacag gaggcguucu gcgggccuac cagcuuaccg     3300

cuccguucga gacauuuaag aagaugggga aacagaccgg cauuaucuac uauguccccg     3360

cgggauucac gucuaagauu uguccuguga ccggcuuugu gaaccaacug uaccccaaau     3420

augaaagcgu cuccaagucu caggaguucu ucagcaaauu ugacaagauc ugcuacaauc     3480

ucgauaaggg guauuucgag uuuuccuucg acuacaaaaa cuuuggagau aaggccgcua     3540

aaggcaagug gacaauugcc ucuuucggga gcagacugau caauuuucgc aacuccgaca     3600

aaaaucauaa cugggauacc cgggaagugu auccuacaaa ggagcuggaa aaacuucuga     3660

aagacuacag caucgaguac ggacacggcg aguguauuaa ggccgcuauc ugcggggaau     3720

cugauaaaaa guucuuugcc aaacucaccu ccguucugaa cacgauucuc cagaugagaa     3780

auagcaagac aggcaccgag cuggacuauc uuaucucucc cguggcggau gucaacggaa     3840

auuucuuuga cucccgccaa gcuccgaaga acaugccuca ggaugccgac gccaauggcg     3900

cuuaccacau ugggcugaaa ggacucaugc ugcugggccg gaucaagaac aaucaggaag     3960

ggaaaaagcu gaaccuugug aucaaaaaug aggaauauuu cgaguuuguu cagaacagaa     4020

auaacaagcg gcccgccgcg acaaagaagg cuggacaagc caagaaaaag aaauaaggac     4080

uagugaaggg aacacccaaa uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg     4140

uaauuguaca agcaguuggu cacccaccau agggcaugau caacaccgca accuuuccuu     4200

uuucccccag ugauucugaa aaaccccucu ucccuucagc uugcuuagau guuccaaauu     4260

uaguaagcuu aaggcggccu acagaagaaa aagaaaaaaa aggccacaaa aguucccucu     4320

cacuuucagu aaauaaaaua aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa     4380

ugaaauaaau auuguguugu gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga     4440

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaagaa uu                                                         4512


<210>  4666
<211>  4813
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4666
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgacaa gaaauacagc      240

auuggacugg auaucggcac caacuccguu gggugggcgg ugauuacaga cgaguauaag      300

gugccgucua agaaauucaa gguccucgga aauacggauc ggcauagcau caaaaagaac      360

cugauuggcg cucugcuguu ugacuccggg gaaaccgccg aggccacacg ccucaaaaga      420

accgcucggc gcagauacac acggcgcaag aauagaaucu gcuaucugca ggaaaucuuc      480

ucuaacgaga uggccaaggu ggaugacagc uuuuuccacc ggcuugaaga guccuuucug      540

guugaggaag auaaaaagca ugagcgccac cccauuuucg gaaauaucgu ggacgaaguc      600

gcuuaccaug agaaauaucc uaccauuuac caccucagaa agaaacuggu ggauucuaca      660

gacaaggccg aucugcggcu caucuaucug gcgcuugccc acaugauuaa auuucgcggc      720

cauuuccuga ucgagggcga ccucaacccc gauaauagcg acguugacaa gcuguuuauu      780

caacuugugc agaccuacaa ccagcuguuc gaagagaauc cuaucaacgc uuccgggguc      840

gaugccaagg cuauccucag cgccagacug ucuaaauccc ggcgccugga aaaucucauu      900

gcgcagcugc ccggagagaa gaaaaacggc cuuuuuggga aucugaucgc ccucagccug      960

ggacuuacgc cuaacuucaa gucuaauuuu gaccuggcug aagaugccaa acuccaacug     1020

uccaaggaca ccuacgauga cgaucuggac aaccuccugg cucagauugg cgaucaguau     1080

gccgaccuuu uccuggccgc uaagaaccuc agcgaugcga uccugcuuuc ugacauucug     1140

agagugaaua cagagaucac caaagccccg cucuccgcua gcaugaucaa gcgguacgac     1200

gagcaccauc aggaucugac acugcucaaa gcccugguga gacaacagcu ucccgaaaag     1260

uauaaagaga uuuucuuuga ccaguccaag aacggguacg ccggauauau cgauggcggc     1320

gcuucucagg aagaguucua caaguuuauu aaaccuaucc uggaaaagau ggacgggacc     1380

gaggagcucc uggucaaacu gaaucgcgaa gaucuucucc ggaagcaaag aacguucgac     1440

aacggaagca uuccccacca gauccaccug ggcgagcugc augccauccu ccgccggcag     1500

gaagauuuuu auccuuuccu gaaagacaau agagagaaga uugaaaagau ccuuacauuu     1560

cgcauucccu acuacguugg gccgcuggcg cggggaaacu ccagauucgc uuggaugacc     1620

cgcaaaagcg aggagacaau caccccuugg aauuuugaag agguggugga uaagggcgcc     1680

ucugcccaau ccuucauuga acggaugacc aacuuugaca aaaaucuccc caacgagaag     1740

guccugccua aacacagccu gcuuuaugag uacuucacag uguauaauga acucacgaag     1800

guuaaauacg ugaccgaggg gaugagaaag cccgcuuuuc ugucuggaga acagaagaaa     1860

gccaucgucg aucuucucuu caagacaaac cgcaaaguga ccguuaagca gcugaaagag     1920

gacuauuuca agaagauuga auguuuugau agcguggaga ucuccggcgu cgaggaccgg     1980

uucaaugcuu cucuuggcac auaccaugac cugcucaaaa ucauuaagga uaaagacuuu     2040

cuggauaacg aagagaauga agacauccug gaggauauug ugcuuacccu cacgcuguuc     2100

gaagacagag agaugaucga ggaacggcug aagacauaug cccaccucuu ugaugacaaa     2160

gucaugaagc agcugaagcg cagacgguac accggguggg gaagacuuag ccgcaaacug     2220

auuaacggca uccgggauaa gcaauccggg aaaaccaucc ucgacuuccu uaagagcgau     2280

ggauuugcga acagaaauuu caugcagcug auucaugacg auucucugac auuuaaagag     2340

gacauccaga aggcccaggu guccggccaa ggggacagcc uccacgaaca cauugcuaac     2400

cuggccggcu cucccgcuau caagaaaggc auucugcaga ccguuaaggu ggucgaugag     2460

cucgugaaag uuaugggacg ccauaagccu gaaaauaucg ugaucgagau ggccagagag     2520

aaccagacaa cgcagaaagg gcaaaagaac ucccgggaac gcaugaaaag aauugaggaa     2580

ggaaucaagg agcugggcag ccagauucuu aaggagcacc cggucgaaaa uacccagcug     2640

cagaaugaga aacucuaccu guauuaccug caaaacgggc gggacaugua uguggaucag     2700

gaacuugaca ucaaucgccu guccgauuac gacguggauc auauuguucc ccagucuuuc     2760

cucaaggacg auagcaucga caacaaaguc cugacaagau ccgauaagaa ucggggaaaa     2820

ucugacaacg ugccuagcga ggaagugguc aagaagauga aaaauuauug gcgccaacuc     2880

cugaacgcga aacuuaucac ccagagaaaa uuugacaauc ugacaaaggc cgagcggggc     2940

gggcucuccg agcuggauaa agcuggcuuc auuaagcgcc agcuggugga aaccagacag     3000

aucacgaagc acguugccca aauucuugac agccggauga acaccaaaua cgaugagaac     3060

gacaagcuga ucagagaagu gaaagucauu acacucaagu cuaaacuggu guccgauuuu     3120

cgcaaggacu uccaguucua uaagguucgg gagaucaaua acuaccacca ugcucacgau     3180

gccuaccuca augccguggu cggaaccgcu cugauuaaaa aguaucccaa acuugaaagc     3240

gaguuugugu acggcgacua uaagguuuac gaugugagaa aaaugaucgc gaagucugag     3300

caggaaaucg ggaaggccac agcuaaauau uucuuuuacu ccaacauuau gaauuucuuu     3360

aagaccgaga ucacgcuggc caacggagaa auucgcaaac ggccucucau cgagacaaau     3420

ggcgaaaccg gggagauugu cugggacaag ggaagagauu ucgccacagu gcgcaaagug     3480

cugagcaugc cccaggucaa caucguuaag aaaaccgagg ugcaaaccgg cggguuuucu     3540

aaggaaagca uccugccgaa gagaaauucc gacaaacuua uugcucggaa gaaagauugg     3600

gacccuaaga aauauggcgg auucgacagc cccacagugg ccuacucugu ccuggugguu     3660

gcgaaggugg agaagggcaa auccaagaaa cucaagagcg ucaaagaacu gcucgggauc     3720

acgauuaugg agcgcuccuc uuuugagaag aacccuaucg auuuccugga agcuaaggga     3780

uacaaagagg ugaagaaaga ccuuauuauc aagcugccca aauauagccu guuugaacuc     3840

gagaauggcc ggaagagaau gcuggccucc gccggggaac uucagaaggg aaacgagcug     3900

gcucucccuu cuaaauacgu uaauuuccug uaucucgcca gccauuacga gaagcugaaa     3960

ggcucccccg aagauaacga gcagaagcag cuuuucgugg aacaacacaa acauuaucug     4020

gacgagauca uugaacagau cucugaguuu agcaagcggg ucauucuggc ggaugcuaau     4080

cucgacaaag ugcuguccgc cuacaacaag caccgcgaua agccgaucag agagcaggcc     4140

gaaaacauua uccaccuuuu cacccugaca aaucucgggg cuccugccgc uuuuaaauau     4200

uucgacacca caauugaucg gaagcgcuac accagcacga aagagguucu ggacgccaca     4260

cucauccauc aaucuaucac cggccuguac gaaaccagaa uugaucuuuc ccagcuggga     4320

ggcgacaagc ggcccgcggc cacaaagaag gcugggcagg ccaagaaaaa gaaauaagga     4380

cuagugaagg gaacacccaa auuuaauuca gccuuaagca caauuaauua agagugaaac     4440

guaauuguac aagcaguugg ucacccacca uagggcauga ucaacaccgc aaccuuuccu     4500

uuuuccccca gugauucuga aaaaccccuc uucccuucag cuugcuuaga uguuccaaau     4560

uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa aaggccacaa aaguucccuc     4620

ucacuuucag uaaauaaaau aaaagcagca acagaaauaa agaaauaaau gaaauucaaa     4680

augaaauaaa uauuguguug ugcagcauua aaaaaucaau aaaaauuaaa aaugagcaag     4740

aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4800

aaaaaaaaga auu                                                        4813


<210>  4667
<211>  5596
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4667
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaacuu caagauucug      240

ccgaucgcga uugaccucgg aguuaaaaau acaggcgugu uuagcgcuuu cuaccagaag      300

gggacauccc uggagcggcu ugauaacaag aauggaaaag uguaugaacu gucuaaggac      360

agcuacacgc uccugaugaa caaucgcacc gccagacggc aucaacgcag aggcaucgau      420

cggaaacagc uugucaagcg ccuguuuaaa cucauuugga cagagcagcu gaaccuggaa      480

ugggacaagg auacccagca agccaucucc uuccucuuua auagacgggg guucucuuuu      540

aucacagacg gauauagccc cgaguaccug aacauugugc cugaacaggu uaaggcuauc      600

cuuauggaua uuuucgacga uuauaauggc gaggacgauc uggacuccua ccucaaacug      660

gccaccgagc aggaaucuaa gaucagcgag auuuauaaca aacuuaugca gaagauccug      720

gaauuuaaac ucaugaagcu gugcacagau auuaaagacg acaagguguc caccaagacg      780

cugaaagaga ucaccagcua cgaguucgaa cuccuggcug auuaccuugc caauuauucu      840

gagucccuga agacacaaaa auuuagcuac accgacaagc agggcaaccu caaagaacug      900

ucuuauuacc accaugauaa guauaauauc caggaguucc uuaagcgcca cgcgacaauu      960

aacgacagaa uccuggauac ccuccugacg gacgaucugg acauuuggaa uuuuaacuuc     1020

gaaaaauucg auuuugacaa gaacgaggag aaacuccaga aucaagaaga uaaggaccau     1080

auccaggccc aucugcacca cuucgucuuu gcugugaaca aaauuaaguc cgagauggcc     1140

agcgggggac ggcaccgcuc ucaguacuuc caagaaauca caaauguucu ugacgagaac     1200

aaucaucagg aaggcuaucu gaagaacuuu ugugagaauc uccacaacaa aaaguacucc     1260

aaucugagcg ugaaaaaccu ugucaaucug aucgggaacc ucuccaaucu ggagcugaag     1320

ccccucagaa aauacuucaa cgauaagauu cacgcuaagg ccgaccauug ggaugaacag     1380

aaauuuaccg agacauauug ccacuggauc cugggagaau ggcggguggg cgugaaggac     1440

caggauaaaa aggacggggc gaaauacucu uauaaggauc uuuguaauga gcugaaacaa     1500

aaggucacca aggccggacu cguugacuuc cugcuggaac uugauccuug ccgcaccauu     1560

cccccuuacc ucgacaacaa caauagaaaa ccgcccaagu gucagagccu gauccugaac     1620

ccuaaauuuc ucgauaauca guaucccaac uggcagcaau accugcagga gcuuaagaaa     1680

cugcagucca uucagaauua ucucgacagc uucgagacag aucugaaggu gcugaagucu     1740

uccaaagacc aaccuuacuu uguggaauac aagagcucua accagcagau cgcuagcggc     1800

cagcgggacu auaaagaucu ugacgccaga auccuccaau ucauuuuuga ucgcgucaag     1860

gcuuccgacg agcugcugcu caaugaaauc uacuuccagg ccaaaaagcu gaagcagaaa     1920

gccucuagcg agcuugagaa gcuggaaucc agcaaaaagc ucgaugaggu gauugcuaac     1980

ucucaacugu cccagauccu gaaaagccag cauacgaaug gcauuuucga acaggggacc     2040

uuucuucacc ucguuugcaa guauuacaag caacggcaga gagcgcgcga cucucggcug     2100

uauaucaugc ccgaguacag auaugauaaa aagcugcaua aauacaacaa uacaggacgc     2160

uucgacgaug acaaccagcu ccugaccuac uguaaucaca agccgcggca gaaaagauau     2220

caacuucuga acgaucucgc cggcgugcug caggucuccc cuaauuuucu gaaggacaaa     2280

auugggagcg augacgaccu uuucaucucc aaguggcucg uggaacacau ccgcggauuu     2340

aagaaggcuu gcgaggauuc ucugaaaauu cagaaagaca accggggccu gcucaaccau     2400

aagaucaaua uugccagaaa cacaaaaggg aagugugaga aggaaaucuu caaucugauu     2460

ugcaaaaucg agggaagcga agauaagaaa ggcaacuaca agcacggccu ugccuaugag     2520

cuggggguuc uccuguuugg agaacccaau gaggcuucca aaccugaguu cgaccgcaag     2580

aucaagaaau uuaacucuau uuacagcuuc gcccagaucc aacagauugc guuugcugaa     2640

cggaagggca augccaacac cugugccgug ugcuccgcug auaaugccca uagaaugcag     2700

caaaucaaaa uuacggagcc cgucgaagac aacaaggaua aaaucauccu gagcgcuaag     2760

gcccagcggc uucccgcgau uccuacacgc aucguggacg gggccgucaa gaaaauggcu     2820

accauucugg ccaagaauau cguggaugac aacuggcaga auauuaaaca gguucucucu     2880

gcuaagcacc aacugcacau cccgaucauu accgagucca acgccuucga auuugagccc     2940

gcgcucgccg augugaaagg aaagagccug aaggacagac ggaaaaaggc ucuugagaga     3000

aucucuccug aaaauauuuu caaagacaag aacaaccgca ucaaagaguu cgccaagggc     3060

auuuccgcuu auagcggggc caaucugaca gauggcgacu uugauggagc caaagaggaa     3120

cucgaccaua ucauuccccg gucucacaag aaguacggca cccugaacga ugaggcuaau     3180

cugaucugcg ucacaagagg ggacaacaaa aauaagggaa accgcaucuu cugucuuaga     3240

gaucuggcgg acaauuauaa acucaagcag uuugaaacga ccgaugaccu ggagauugag     3300

aaaaagaucg ccgauacaau uugggacgcu aacaagaaag auuucaaguu uggcaauuac     3360

cggagcuuca ucaaccucac cccucaggaa cagaaagccu uucgccaugc ccuguuccuu     3420

gcugacgaaa aucccauuaa gcaagccgug aucagagcga ucaacaaucg gaaccgcaca     3480

uuuguuaaug ggacccagag auacuucgcu gaggugcugg ccaacaauau uuaucuccgg     3540

gccaaaaagg agaaccugaa cacggacaag aucuccuuug auuacuucgg cauuccgacc     3600

aucggaaaug ggcgcggcau ugcugaaauc agacagcugu augagaaagu cgacagcgau     3660

auccaggccu acgcgaaggg agacaaaccu caggcuucuu auucccaccu uauugaugcc     3720

augcuggccu ucugcaucgc ugccgacgaa caucggaacg auggcagcau ugggcucgag     3780

aucgacaaga auuacucccu guauccccuc gauaaaaaca caggagaagu guuuaccaag     3840

gacauuuucu cucaaaucaa gauuacagau aaugaguuua gcgacaaaaa gcuggugcgc     3900

aaaaaggcua ucgagggcuu caacacccac agacagauga cgcgggaugg gaucuacgcc     3960

gaaaauuacc uuccuauucu gauccacaaa gagcugaacg aaguuagaaa gggauauaca     4020

uggaaaaauu ccgaggaaau uaagaucuuu aagggcaaaa aguacgacau ucagcaacuc     4080

aacaaucugg ucuauugucu uaaauucgug gacaagccca ucucuaucga uauucagauc     4140

agcacccugg aggagcuccg caacauucug acaaccaaua acaucgcggc caccgcugaa     4200

uacuauuaca uuaaucucaa aacacagaag cugcaugagu auuacaucga aaacuacaac     4260

acggcccuug gguauaagaa auacuccaag gagauggagu uucugcgguc ucuggcuuau     4320

agaagcgaac gcgugaaaau caaguccauu gacgauguca aacaagugcu cgacaaggau     4380

agcaauuuca ucauuggcaa gaucacccug ccuuuuaaaa aggaguggca gcggcuuuac     4440

agagaauggc agaacacaac cauuaaagac gauuaugagu uccugaaguc uuucuucaau     4500

guuaaaucca ucacaaagcu ccacaagaaa gugcgcaagg acuuuagccu gcccauuucu     4560

accaacgaag gaaaauuccu cgucaagcgg aaaacguggg auaauaacuu caucuaccag     4620

auccugaaug acuccgauag cagagccgac ggcacaaagc cguuuauucc ugccuucgac     4680

aucucuaaaa acgagauugu ggaggcuauc auugauuccu uuaccagcaa gaauaucuuc     4740

uggcuuccca agaacaucga acugcaaaaa guugacaaua agaacauuuu ugcgaucgau     4800

accuccaaau gguucgaggu ggaaacaccc agcgaccugc gcgauauugg gaucgccacc     4860

auucaguaua agaucgacaa uaacucucgg ccuaaaguca gagugaagcu cgauuacguu     4920

aucgacgaug acagcaagau uaauuacuuu augaaccauu cccugcuuaa aucucgguau     4980

ccggauaagg ugcuggagau ccucaaacag agcacaauua ucgaauuuga guccucugga     5040

uuuaacaaga cgauuaaaga gaugcugggc augaagcucg cugggaucua caaugaaacc     5100

agcaacaaua agcgccccgc cgccaccaaa aaggcuggac aggccaaaaa gaaaaaguaa     5160

ggacuaguga agggaacacc caaauuuaau ucagccuuaa gcacaauuaa uuaagaguga     5220

aacguaauug uacaagcagu uggucaccca ccauagggca ugaucaacac cgcaaccuuu     5280

ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu cagcuugcuu agauguucca     5340

aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa aaaaaggcca caaaaguucc     5400

cucucacuuu caguaaauaa aauaaaagca gcaacagaaa uaaagaaaua aaugaaauuc     5460

aaaaugaaau aaauauugug uugugcagca uuaaaaaauc aauaaaaauu aaaaaugagc     5520

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5580

aaaaaaaaaa agaauu                                                     5596


<210>  4668
<211>  3868
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4668
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagcg gaacuacauu      240

cugggacucg acaucggcau uaccagcguu ggguauggaa ucauugauua cgagacacgc      300

gacgugaucg augcgggcgu gagacuguuc aaagaagcua augucgagaa caaugaaggg      360

cggcgcucca agagaggagc ccggcgccuu aagagacggc gcagacaucg gauccagcgc      420

gugaaaaagc ugcucuuuga cuauaaccug cuuacggauc acucugagcu gagcggcauu      480

aauccguacg aagccagagu uaaaggccuc ucccaaaagc ugucugagga ggaguucagc      540

gcugcccugc uccaucuggc uaaacggcgc ggggugcaca acgucaauga aguggaagag      600

gacaccggaa acgagcuuuc cacaaaggaa cagaucucua gaaauagcaa ggcccuggag      660

gaaaaauaug uugcggagcu ccagcuggaa cggcuuaaga aagauggcga ggugagaggg      720

uccauuaacc gcuuuaagac cagcgacuac gucaaagagg ccaagcagcu gcucaaagug      780

caaaaggcuu aucaucagcu ggaucagucu uucaucgaca cauacauuga ucugcucgaa      840

acccggagaa cauacuauga gggacccggc gaaggguccc cuuuuggaug gaaggacauc      900

aaagaguggu acgaaaugcu gaugggccac ugcaccuauu uccccgagga gcuucgcagc      960

gugaaguacg ccuauaaugc ugaucuguac aacgcccuca augaccugaa caaucuuguc     1020

auuacgcggg acgaaaacga gaaacuggaa uauuacgaga aguuucagau caucgaaaac     1080

guuuucaaac aaaagaagaa accuacccuc aagcagauug cgaaagagau ccuggugaau     1140

gaggaagaua uuaagggcua cagagugaca ucuaccggga aacccgaguu uacaaaccug     1200

aaggucuauc acgacaucaa ggauauuacc gcccgcaaag aaaucaucga gaaugcugag     1260

cuccuggacc agauugccaa gauccuuacg auuuaccagu ccagcgaaga uauccaagag     1320

gaacugacaa accucaauuc ugagcugacc caggaagaga uugagcagau cuccaaccuu     1380

aaaggauaua caggcaccca uaaucugagc cucaaggcua ucaaccugau ucuggacgaa     1440

cucuggcaca ccaaugauaa ccaaaucgcc auuuucaauc ggcugaaacu ugugccuaag     1500

aagguugacc ugucccagca gaaagagauc ccgacaacgc ucguggauga cuuuauucug     1560

ucucccgucg ugaagcgcag cuucauccag uccauuaaag uuaucaacgc caucauuaag     1620

aaauacgggc ugccuaauga uaucauuauc gaacuugcuc gcgagaagaa cagcaaagac     1680

gcgcaaaaga ugauuaauga aaugcagaag cggaacagac agaccaacga gcgcaucgag     1740

gaaaucauuc ggacaaccgg aaaagagaau gccaaguauc ucaucgagaa aauuaagcug     1800

caugacaugc aggaaggcaa augucuguac ucucucgaag cuaucccccu ggaggaucuu     1860

cugaacaauc cuuuuaacua ugagguggac cacauuaucc ccagauccgu cagcuucgau     1920

aauucuuuca acaauaaggu gcucgucaag caagaagaga acagcaaaaa ggggaaucgg     1980

acaccguuuc aguaccuguc cucuagcgac uccaaaauca gcuacgaaac cuucaagaaa     2040

cacauucuga accuugccaa gggaaagggc cgcaucucua aaacgaagaa agaguaucuc     2100

cuggaggaaa gagauauuaa ucgguuuucc gugcagaagg acuucaucaa cagaaaucug     2160

guugauacac gcuacgccac ccggggccuc augaaccugc uuagaagcua uuuucgcgug     2220

aauaaccugg acgucaaagu gaagucuauu aacgggggau ucaccuccuu ucucagacgg     2280

aaguggaaau ucaagaaaga gcgcaauaag ggcuacaaac aucacgcuga agaugcccug     2340

aucaucgcga acgcugacuu uauuuucaag gaguggaaaa agcuggauaa ggccaaaaag     2400

guuauggaaa aucagauguu ugaggagaaa caagccgaaa gcaugccuga gaucgaaaca     2460

gagcaggaau auaaggagau uuucaucaca ccccaucaga uuaaacacau caaggacuuu     2520

aaggauuaca aauauuccca uagaguggac aagaaaccua accgggagcu uauuaaugac     2580

acccucuacu cuacgcgcaa ggaugacaaa gggaacaccc ugaucgucaa uaaccugaau     2640

ggacucuacg auaaggacaa cgauaagcug aagaaacuua ucaauaaaag ccccgaaaag     2700

cugcucaugu aucaccacga cccccagaca uaccaaaaac ugaagcugau uauggagcag     2760

uauggcgaug aaaagaaccc ucuuuacaaa uauuacgagg aaaccgggaa uuaucucaca     2820

aaguacucca aaaaggacaa cggcccggug aucaaaaaga uuaaguacua uggaaauaaa     2880

cugaacgcuc aucuggauau caccgacgau uaccccaacu cuagaaauaa ggugguuaaa     2940

cucagccuga agccuuaucg guucgacguc uaccuugaua acggcgugua uaaauucgug     3000

acggucaaga aucuggacgu gauuaaaaag gagaacuacu augagguuaa uuccaagugc     3060

uacgaagagg ccaaaaagcu caaaaagauc agcaaccagg cugaauuuau cgccucuuuc     3120

uacaauaacg accugauuaa aaucaauggg gagcuguauc gcgugauugg agucaacaau     3180

gaucuucuga acagaaucga ggugaauaug auugacauca ccuaccggga auaucucgag     3240

aacaugaaug auaagagacc cccuagaauc auuaagacaa ucgcguccaa aacccaaagc     3300

auuaagaaau acucuacaga cauccugggc aaccucuaug aaguuaaguc caaaaagcac     3360

ccccagauua ucaagaaagg gaagcggccg gccgcuacca aaaaggccgg acaggcuaaa     3420

aagaagaaau aaggacuagu gaagggaaca cccaaauuua auucagccuu aagcacaauu     3480

aauuaagagu gaaacguaau uguacaagca guuggucacc caccauaggg caugaucaac     3540

accgcaaccu uuccuuuuuc ccccagugau ucugaaaaac cccucuuccc uucagcuugc     3600

uuagauguuc caaauuuagu aagcuuaagg cggccuacag aagaaaaaga aaaaaaaggc     3660

cacaaaaguu cccucucacu uucaguaaau aaaauaaaag cagcaacaga aauaaagaaa     3720

uaaaugaaau ucaaaaugaa auaaauauug uguugugcag cauuaaaaaa ucaauaaaaa     3780

uuaaaaauga gcaagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        3868


<210>  4669
<211>  4936
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4669
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcccuguu caacaagugc      240

auuaucauua gcaucaaucu cgacuuuucc aacaaagaga aguguaugac caagccguac      300

ucuauuggac uggauaucgg cacaaauagc guuggguggg cggugaucac ggacaacuau      360

aaagugcccu ccaagaaaau gaagguccuu ggaaauaccu cuaaaaagua cauuaagaaa      420

aaccugcucg gcgugcugcu uuucgauagc gggaucacag cugaaggacg gcgccugaag      480

agaaccgccc ggcgcagaua uacacggcgc agaaaucgga uucucuaccu gcaggagauc      540

uuuuccaccg aaauggccac acuggacgau gcuuucuuuc aacgccucga cgauucuuuc      600

cugguuccug acgauaaaag agacagcaag uaucccauuu uuggcaaccu uguggaggaa      660

aaagucuacc augaugaguu cccuaccauc uaucaucugc ggaaguaccu cgccgacucc      720

acgaaaaagg cugaucugcg ccuuguguac cuggcccucg cgcacaugau uaaguauaga      780

ggccacuuuc ugaucgaggg ggaguucaau agcaaaaaca augacaucca gaagaacuuu      840

caggacuucc uggauaccua caaugccauu uuugaaucug accucucccu ggaaaacagc      900

aaacagcuug aggagaucgu uaaggauaaa auuucuaagc uggaaaagaa agaccggauc      960

cucaagcugu uccccggaga gaaaaauucc ggcauuuuca gcgaauuucu uaagcugauc     1020

guggggaacc aagcugauuu ccgcaaaugc uuuaaccucg acgagaaggc cucucugcau     1080

uucuccaagg aaagcuauga ugaggaccug gagacacucc ugggauacau cggcgaugac     1140

uauuccgaug ucuuucuuaa agcuaagaaa cuguacgacg ccauucuccu gucuggguuc     1200

cuuaccguga cagacaauga aaccgaggcg ccucugagcu ccgccaugau caagagauau     1260

aacgaacaca aagaggaucu cgcucugcug aaggaauaca uucggaauau cagccucaag     1320

acguacaacg agguguuuaa agacgauaca aagaauggau augccggcua cauugacggc     1380

aaaaccaacc aggaggauuu cuaugucuac cugaagaauc uucuggcuga auuugagggg     1440

gccgacuauu uccucgaaaa gaucgauaga gaggacuuuc ugcgcaagca gcggacauuc     1500

gauaacggau cuaucccgua ccagauucau cugcaagaaa ugagagccau ccuugacaaa     1560

caggcuaagu uuuaucccuu ccucgcgaag aauaaagagc gcauugagaa gauccugacc     1620

uuccggauuc cuuacuacgu uggcccccug gccagaggga acuccgauuu ugcuuggagc     1680

auccgcaaac ggaaugaaaa gauuaccccu uggaacuucg aggacgugau cgauaaagaa     1740

ucuagcgccg aggccuuuau caauagaaug acauccuucg accucuaucu gcccgaggaa     1800

aaggugcuuc cgaagcacuc ucugcucuac gagacguuua acgucuauaa ugaacugacc     1860

aaagugcgcu ucauugcuga gagcaugcgg gacuaccagu uucuggauuc caagcagaaa     1920

aaggacaucg uuagacuuua uuucaaagau aagcgcaagg ugacagacaa agauauuauc     1980

gaauaccucc acgccauuua uggauacgac ggcaucgagc ugaaggggau cgagaaacaa     2040

uuuaacagcu cucuguccac cuaccaugau cuccugaaca uuaucaauga caaggaguuc     2100

cuugaugaca gcucuaacga agcgauuauc gaagagauua uccacacacu gaccaucuuu     2160

gaagaucggg agaugauuaa acagagacuc uccaaguucg agaauaucuu ugacaagagc     2220

guccugaaaa agcugucccg gcgccacuau acgggauggg gcaaacuuuc ugcuaagcuc     2280

auuaacggca ucagagauga aaagagcggg aauacaauuc uggacuaccu gaucgacgau     2340

ggaaucucca accggaauuu caugcagcuc auucaugacg augcccuguc uuucaagaaa     2400

aagauccaaa aggcccagau uaucggcgac gaggauaaag ggaacauuaa ggaagugguu     2460

aaaagccuuc cuggaucccc cgcuaucaag aaaggcauuc ugcagagcau caagaucgug     2520

gacgagcucg ucaaggugau gggcgggaga aaaccugaau cuauugucgu ggagauggcc     2580

cgcgagaauc aguauaccaa ccaaggaaag uccaauagcc agcagcggcu gaaaagacug     2640

gaaaagucuc uuaaagagcu cggcuccaag auccugaagg aaaacauucc cgcuaaacug     2700

agcaagaucg auaauaacgc ccuccagaau gaccgccugu accuuuauua ccugcaaaac     2760

gggaaagaua uguauaccgg agacgaucuc gacauugaca gacugucuaa cuacgauauc     2820

gaccacauca uuccccaggc guuucugaag gauaauagca ucgacaacaa aguucuugug     2880

uccagcgccu cuaaucgggg caaguccgau gacuucccua gccuggaggu cgugaagaaa     2940

cgcaagacau uuugguacca gcuccugaaa uccaagcuca uuucucagag aaaauucgau     3000

aaccugacca aggcugagcg cgggggacuu cugccggaag acaaggccgg cuuuauccaa     3060

cggcagcucg uugagacaag acagauuacg aaacaugugg cucggcugcu ggaugaaaag     3120

uucaauaaca aaaaggacga gaauaaccgc gccgucagaa ccgugaaaau caucacacuu     3180

aagagcaccc ugguguccca guuucggaaa gauuucgaac ucuauaaggu ucgcgagauu     3240

aaugacuuuc accaugcgca cgaugccuac cugaacgcug ucaucgccuc ugcucuccug     3300

aagaaauauc ccaaacuuga gccugaguuc guguacgggg acuaucccaa auacaauagc     3360

uuuagagaac ggaaguccgc cacagaaaaa guguauuucu acucuaacau uaugaauauc     3420

uucaaaaaga gcauuucccu ggccgacggc agagucaucg agcgcccucu cauugaagug     3480

aacgaggaga ccggagaaag cguuuggaau aaagagucug aucuggcuac ggugcggaga     3540

guccuguccu acccccaagu gaacguugug aagaaagucg aggaacagaa ccacggccuu     3600

gaccgcggga agccgaaagg acuguuuaau gcgaaccuca gcucuaagcc uaagcccaau     3660

uccaacgaaa aucugguggg cgccaaagag uaucucgauc cuaagaaaua cgggggauau     3720

gcuggcauca gcaacucuuu cgccguucug gugaagggga ccaucgagaa aggcgccaag     3780

aagaaaauua caaauguccu ugaauuucag ggaaucucca uucuggaccg gaucaacuac     3840

agaaaggaua aacugaauuu ccuccuggag aagggcuaua aagacauuga acuuaucauc     3900

gagcugccca aguacagccu cuuugagcug uccgauggga gccgccggau gcucgcuucu     3960

auucugagca ccaacaauaa aagaggagaa auccauaagg gcaaccaaau uuuccuuucc     4020

cagaaguuug ugaaacugcu guaucacgcc aagcgcaucu cuaauacaau uaacgagaau     4080

caucggaaau acguggaaaa ccacaagaaa gaguucgagg aacucuuuua cuauauccug     4140

gaguucaaug aaaacuacgu cggggcgaag aagaacggaa aacuucugaa uagcgcuuuu     4200

caguccuggc agaaccacuc uaucgacgag cucuguagcu ccuucauugg cccuaccggg     4260

ucugaaagaa agggccuguu ugagcucacg agccggggau ccgccgccga uuucgaguuc     4320

cugggcguua aaaucccccg cuauagagac uacacaccga gcucucuucu gaaggaugcu     4380

acccugauuc aucaauccgu gacagggcuc uaugaaaccc ggaucgaccu ggccaaacuu     4440

ggagagggca agcgcccugc ggcuaccaag aaagccgggc aggccaagaa aaagaaauaa     4500

ggacuaguga agggaacacc caaauuuaau ucagccuuaa gcacaauuaa uuaagaguga     4560

aacguaauug uacaagcagu uggucaccca ccauagggca ugaucaacac cgcaaccuuu     4620

ccuuuuuccc ccagugauuc ugaaaaaccc cucuucccuu cagcuugcuu agauguucca     4680

aauuuaguaa gcuuaaggcg gccuacagaa gaaaaagaaa aaaaaggcca caaaaguucc     4740

cucucacuuu caguaaauaa aauaaaagca gcaacagaaa uaaagaaaua aaugaaauuc     4800

aaaaugaaau aaauauugug uugugcagca uuaaaaaauc aauaaaaauu aaaaaugagc     4860

aagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4920

aaaaaaaaaa agaauu                                                     4936


<210>  4670
<211>  4012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4670
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccuggua cgcgagccug      240

auguccgcuc aucaccuccg gguuggaauu gacgugggca cccauucugu ggggcuggcc      300

acacuucgcg ucgaugacca cggaacgccg aucgagcugc ucagcgcccu gucccauauu      360

cacgauucug gcguggggaa ggaagggaaa aaggaccacg auaccagaaa gaaacuuagc      420

ggcaucgcuc ggcgcgccag acggcugcuc caucaccgca gaacacagcu gcaacagcug      480

gacgagguuc uccgggaucu gggcuucccc auuccuaccc ccggggaauu ucuugaccug      540

aacgagcaga cagauccuua ucgcgugugg agaguccggg cucgccucgu ggaagagaag      600

cugcccgagg aacuuagagg accugccauc uccauggcgg uucggcauau cgcccgccac      660

agaggcuggc ggaauccgua cucuaaagug gagagccugc ucucccccgc ugaagagagc      720

ccuuucauga aggcccugag agagcgcauu cuggcuacca caggggaagu ccucgacgau      780

ggaaucaccc ccggccaggc cauggcgcaa guggcccuga cgcacaacau uucuaugcgg      840

gggccugagg gaauccuugg caaacugcau caguccgaca augcuaacga aauuagaaag      900

aucugcgccc gccagggcgu gagccccgau gucuguaagc agcuccugcg ggcuguuuuu      960

aaagccgacu cuccgagagg guccgccgug agccgcgugg cuccugaccc ccuuccugga     1020

caaggcucuu uccggagagc gcccaagugc gaucccgagu uucagcgcuu ccggauuauc     1080

uccaucgucg ccaaucugag aauuagcgaa accaaagggg agaaccgccc ucucacagcu     1140

gacgagcgga gacacguggu uaccuuucug acagaagauu cccaggccga ccugaccugg     1200

guggaugucg ccgagaagcu cggagugcau cggcgcgacc ugagaggcac ggcuguucac     1260

acagaugacg gggaacgguc ugccgcgaga ccgcccaucg augcuaccga ccgcauuaug     1320

cggcagacaa aaaucagcuc ccuuaagacc uggugggagg aagccgauag cgagcaaaga     1380

ggagccauga uucgcuaucu guacgaggac ccuaccgacu cugaaugugc ugagaucauc     1440

gccgaacucc ccgaggaaga ucaggcuaaa cuggacuccc uucaucugcc ugccggcaga     1500

gcggccuaua gccgggaguc ucucacagcu cugagcgauc acaugcuggc cacgaccgac     1560

gaucuccacg aggcucgcaa gagacuguuc ggcguggacg auuccugggc gcccccggcc     1620

gaagccauua augcuccugu cgggaacccc ucuguggacc ggacacuuaa gaucgucgga     1680

cgcuaccuga gcgccgugga guccaugugg ggcaccccug aaguuauuca uguggagcac     1740

gucagagaug gguuuacaag cgagcggaug gcugacgaac gcgauaaagc caauagacgg     1800

cgcuauaacg acaaucagga ggccaugaag aaaauccaaa gagauuacgg aaaggaaggc     1860

uacauuucuc ggggagacau cgugagacuc gacgcucugg agcugcaggg cugcgcgugu     1920

cuuuauugcg gaaccacgau cggcuaccau acaugucagc ucgaucacau uguuccccag     1980

gccgggccug gauccaacaa ucgccggggc aaccuggugg cugucugcga aagauguaau     2040

cgcagcaaau cuaacacccc cuucgccgug ugggcccaaa agugcgggau cccgcacgug     2100

ggaguuaagg aggcuauugg ccgggucaga ggguggcgca aacagacccc uaacacaucc     2160

agcgaggacc ugacccggcu caagaaagaa gugaucgcca gacugcgccg gacacaggag     2220

gaucccgaaa uugacgagag auccauggaa ucuguggcgu ggauggcuaa ugagcuucau     2280

caccggaucg ccgccgcuua ucccgagacg accgucaugg uguaccgcgg cagcauuaca     2340

gccgcggcua gaaaggccgc cggaaucgau ucccggauca accugauugg cgaaaaaggg     2400

cgcaaggaca gaaucgaucg gagacaucac gcuguugacg ccucuguggu cgcucucaug     2460

gaggccagcg uggcgaagac ccuggccgaa cgcuccagcc ugcggggaga gcagagacuu     2520

acaggcaaag aacaaaccug gaagcaguau acggggucua ccguuggagc ucgcgagcau     2580

uuugagaugu ggcggggcca caugcuccac cugacagaac uguucaauga gagacucgcc     2640

gaagauaaag uguacgucac ccagaacauu cgccugcggc uuuccgacgg gaaugcucau     2700

acagugaacc cuagcaagcu gguuucucac agacucggcg auggacugac cgugcagcaa     2760

aucgaccgcg ccuguacgcc ggcccugugg ugcgcucuua caagagagaa agauuuugac     2820

gagaagaaug gccuccccgc gcgggaagac cgcgccauua gaguccaugg gcacgagauc     2880

aaguccagcg auuauaucca gguguucucu aaacggaaga aaaccgacag cgaucgcgac     2940

gaaacaccuu ucggagcuau ugccgugaga ggcggguuug ucgagaucgg acccuccauu     3000

caucacgccc ggaucuacag aguugaaggc aagaaaccug uguacgcuau gcugcgcgug     3060

uucacccacg aucugcucag ccagcggcau ggggaccugu uuucugccgu cauucccccu     3120

caguccauca gcaugagaug cgcggagccc aaacuucgca aagcuaucac cacaggcaac     3180

gccacguauc ugggaugggu gguugugggc gaugagcucg aaauuaaugu cgacuccuuc     3240

accaaguacg ccaucgggcg guuucuggag gauuucccga acacaaccag auggcggauu     3300

uguggauaug acacaaauuc uaagcugacc cuuaaaccua ucgugcucgc ugccgaaggc     3360

cuggagaacc ccagcuccgc gguuaaugaa auuguggagc ugaaggggug gcgcgucgcu     3420

aucaacgugc ucacgaaagu ucacccuaca guggucagac gggaugcccu gggacgcccc     3480

agauacucua gccgguccaa ucuuccgacc ucuuggacca ucgagaagcg cccugccgcu     3540

acaaagaagg ccggccaagc uaagaaaaag aaauaaggac uagugaaggg aacacccaaa     3600

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu     3660

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa     3720

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu     3780

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     3840

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     3900

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     3960

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             4012


<210>  4671
<211>  4873
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4671
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaccaa gccguacagc      240

auuggacugg acaucggcac aaacuccguu gggugggcgg ugacgaccga uaauuauaaa      300

gugcccucua agaagaugaa aguccucgga aacacaagca agaaauacau uaagaaaaau      360

cugcuuggcg ugcuccuguu cgacuccggg aucaccgcug agggacggcg ccugaagaga      420

acagcccggc gcagauauac ccggcgcaga aaccggauuc uuuaccugca ggaaaucuuu      480

ucuacagaga uggccacccu cgaugacgcu uucuuucaac gccuggauga cagcuuccug      540

guuccugaug acaagagaga uuccaaauau cccaucuuug gcaaucucgu ggaagagaag      600

gccuaccaug acgaguuccc uacgauuuau caucugcgga aauaccuugc ugauucuacc      660

aagaaagccg accugcgccu cgucuaccug gcgcuugccc acaugaucaa guauagaggc      720

cacuuucuga uugaggggga guucaacagc aaaaauaacg auauccagaa gaauuuucag      780

gacuuccucg acacauacaa cgcuauuuuu gaauccgauc ugagccugga aaauucuaag      840

cagcucgaag agaucgugaa agacaagauu uccaaacugg agaagaaaga ucggauccuu      900

aagcuguucc ccggagaaaa gaacagcggc aucuuuucug aguuccucaa acugauuguu      960

gggaaucaag ccgacuuccg caagugcuuu aaccuugaug aaaaggcuuc ccugcauuuc     1020

agcaaagagu cuuaugacga agaucucgag acccugcugg gauacaucgg cgacgauuau     1080

uccgacgugu uucucaaagc caagaagcug uacgaugcga uucuucugag cggguuccuc     1140

acagucaccg acaaugagac ggaagccccu cuguccucug cuaugaucaa aagauauaac     1200

gagcacaagg aagaccuugc ccugcucaaa gaguacauuc ggaacaucag ccugaagaca     1260

uacaaugaag uguuuaaaga ugacaccaag aacggauaug cuggcuacau cgauggcaag     1320

acaaaucagg aggacuucua uguguaccug aaaaagcucc uggccgaguu ugaaggggcc     1380

gauuauuucc uugagaaaau ugacagagaa gauuuucugc gcaagcagcg gaccuucgac     1440

aacggaucca ucccguacca gauucaccuc caagagauga gagcuauccu ggauaaacag     1500

gcgaaguuuu aucccuuccu ggccaaaaau aaggaacgca uugagaagau ccuuaccuuu     1560

cggaucccuu acuacgucgg cccccucgcu agagggaaca gcgacuucgc cuggucuauu     1620

cgcaaacgga augagaagau cacaccuugg aacuucgaag auguuauuga caaagagucc     1680

agcgccgagg cuuuuaucaa uagaaugacg ucuuucgauc uguaucugcc cgaagagaag     1740

gugcucccga aacauagccu gcuuuacgaa accuuuaacg uguauaauga gcugacaaag     1800

guccgcuuca uugccgaauc caugcgggac uaccaguuuc ucgacucuaa gcagaaaaag     1860

gauaucguga gacuguauuu caaagacaag cgcaaaguua ccgauaagga cauuaucgag     1920

uaccugcacg cgaucuaugg auacgauggc auugaacuua aggggaucga gaaacaauuu     1980

aacagcuccc ucagcacaua ccaugaccug cugaauauua ucaacgauaa ggaguuccuc     2040

gacgauucuu ccaaugaagc uauuaucgag gaaaucauuc acacccugac gaucuuugag     2100

gaccgggaaa ugauuaaaca gagacuuagc aaguucgaga acaucuuuga uaaaucugug     2160

cugaagaagc ucucccggcg ccacuauaca ggauggggca aacugagcgc caagcugauu     2220

aacggcauca gagacgagaa auccgggaau accauccuug auuaccucau ugacgacgga     2280

aucucuaacc ggaauuucau gcagcugauu caugaugacg cccugagcuu uaagaaaaag     2340

auccaaaaag cucagauuau cggcgaugaa gacaagggga acaucaagga ggucgugaaa     2400

ucccucccug gaucucccgc cauuaagaaa ggcauccugc agagcauuaa gaucguugau     2460

gaacuuguga aagucauggg cgggagaaag ccugagucca uuguggucga aauggcucgc     2520

gagaaucagu auaccaacca aggaaagagc aauucucagc agcggcugaa aagacucgag     2580

aagucccuga aagaacuggg cagcaagauc cuuaaagaga acauucccgc caagcucucu     2640

aagaucgaca auaacgcgcu gcagaaugau cgccuguacc ucuauuaccu gcaaaacggg     2700

aaagacaugu auacaggaga ugaccuugau aucgacagac uguccaauua cgacauugau     2760

cacaucauuc cccaggccuu ccucaaggac aacagcaucg auaauaaagu gcugguuucu     2820

agcgcuucca accggggcaa gagcgacgau gugccuucuc uggaagucgu gaaaaagcgc     2880

aagaccuucu gguaccagcu ucugaaaucc aagcucauua gccagagaaa auuugacaac     2940

cugacaaagg ccgagcgcgg gggacucucc ccggaggaua aagcuggcuu cauccaacgg     3000

cagcugguug aaacgagaca gaucaccaag cauguggccc ggcuucugga cgagaaguuu     3060

aauaacaaaa aggaugaaaa uaaccgcgcg gucagaacag ugaaaauuau cacccucaag     3120

ucuacacugg ugagccaguu ccggaaagac uuugagcugu auaagguucg cgaaauuaau     3180

gauuuccacc augcccacga cgcuuaccuu aacgccgucg uggcuuccgc ccugcucaaa     3240

aaguauccca agcuggagcc ugaguuugug uacggggauu aucccaaaua caauucuuuc     3300

agagaaagaa agagcgccac cgagaaaguc uauuuuuacu ccaacaucau gaauauuuuc     3360

aagaaaucua ucagccucgc ugacggccgg gugaucgaac gcccucugau ugagguuaac     3420

gaagagacgg gagaguccgu guggaauaag gaaagcgacc uugcgaccgu ccggagagug     3480

cugucuuacc cccaaguuaa cguggucaag aaaguggagg aacagaauca cggccucgau     3540

cgcgggaagc cgaaaggacu guuuaacgcc aaucugucca gcaagccuaa acccaacucu     3600

aacgagaauc uuguuggcgc uaaggaauau cuggacccua agaaauacgg gggauaugcc     3660

ggcaucucca acagcuucac agugcucguc aaggggacca uugagaaagg cgccaagaaa     3720

aagaucacaa augugcugga guuccaggga auuucuaucc ucgaucggau uaacuacaga     3780

aaggacaaac ugaauuuucu ucuggaaaag ggcuauaaag auaucgagcu gaucauugaa     3840

cuccccaagu acucccuguu cgagcuuagc gacggguccc gccggaugcu ggcuagcauc     3900

cucucuacca acaauaaaag aggagagauu cauaagggca accaaaucuu ucugagccag     3960

aaauucguga agcuccugua ucacgccaag cgcauuucca auacgaucaa cgaaaaucau     4020

cggaaauacg ucgagaacca caagaaagaa uuugaggaac uuuucuacua uauccuggag     4080

uuuaaugaga acuacguugg ggcgaagaaa aauggaaagc ugcucaacuc ugcuuuccag     4140

agcuggcaga aucacuccau ugaugaacug uguucuagcu uuaucggccc uacagggucc     4200

gagagaaagg gccuuuucga acugaccucu cggggaagcg ccgccgacuu ugaguuccuc     4260

ggcgugaaaa uuccccgcua uagagauuac acaccgucca gccugcucaa ggacgcuacc     4320

cugauccauc aaucugugac cgggcuuuau gaaacacgga uugaucuggc caaacuggga     4380

gagggcaagc gcccugcggc uacgaaaaag gccgggcagg ccaagaaaaa gaaauaagga     4440

cuagugaagg gaacacccaa auuuaauuca gccuuaagca caauuaauua agagugaaac     4500

guaauuguac aagcaguugg ucacccacca uagggcauga ucaacaccgc aaccuuuccu     4560

uuuuccccca gugauucuga aaaaccccuc uucccuucag cuugcuuaga uguuccaaau     4620

uuaguaagcu uaaggcggcc uacagaagaa aaagaaaaaa aaggccacaa aaguucccuc     4680

ucacuuucag uaaauaaaau aaaagcagca acagaaauaa agaaauaaau gaaauucaaa     4740

augaaauaaa uauuguguug ugcagcauua aaaaaucaau aaaaauuaaa aaugagcaag     4800

aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4860

aaaaaaaaga auu                                                        4873


<210>  4672
<211>  3955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4672
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgcggc uuucaagccg      240

aacagcauua auuacauccu gggacucgac auuggcaucg ccuccguugg gugggccaug      300

guggagauug augaagagga aaaccccauc cggcugaucg accuuggagu gcgcgucuuu      360

gagagagcug aagugccuaa aaccggcgau ucucuggcca uggcucggcg ccucgccaga      420

agcguucggc gccugacaag acggcgcgcg cauagacuuc ugcggacgcg cagacuccug      480

aagcgggagg gggugcugca ggccgcuaau uucgacgaga acggacucau uaagucccug      540

cccaauaccc cuuggcaacu ucgcgccgcu gcccuggaua gaaaacucac accccuggaa      600

uggucugcgg uccuucugca ccucaucaag caucggggcu aucugagcca gcgcaaaaac      660

gagggcgaaa ccgccgacaa ggagcugggg gcucuccuga aaggaguggc cggcaaugcu      720

cacgcccuuc agacagggga uuuuagaacc ccugccgagc uggcucucaa caaguucgaa      780

aaggaguccg gacauauucg gaaucagaga ucugacuaca gccacacauu uucccgcaaa      840

gaucugcaag cggaacuuau ccuccuguuc gagaagcaga aagaauuugg caacccgcac      900

guuagcgggg gacugaagga gggcauugag acccugcuca ugacgcagcg gcccgcccug      960

ucuggcgacg cugugcagaa aaugcuuggg cauugcaccu ucgaaccugc cgagcccaag     1020

gccgcuaaaa auacauauac cgccgaaaga uuuaucuggc ugacaaagcu caacaaucug     1080

cgcauucuug agcaaggauc cgaacggccu cugaccgaua cggagagagc gacacucaug     1140

gacgagcccu accgcaagag caaacugacc uaugcucagg cccggaagcu gcucggccug     1200

gaagauacag ccuucuuuaa agggcuuaga uacggaaagg acaacgcuga ggccucuacc     1260

cugauggaaa ugaaggcuua ucacgccauc ucccgcgcgc ucgagaagga aggccugaag     1320

gacaaaaaga gcccgcugaa ucuuucuccu gagcuccagg augagaucgg gaccgccuuc     1380

ucccuguuua aaacagacga agauauuacg ggacggcuga aggacagaau ccagcccgag     1440

auucucgaag cucugcuuaa acauaucagc uucgauaagu ucguccaaau uucccugaag     1500

gcccuccggc gcaucgugcc ucugauggag cagggcaaaa gauacgacga ggcuugugcc     1560

gaaaucuacg gcgaucacua ugggaagaaa aacaccgagg aaaagauuua ccugccuccc     1620

auccccgcgg acgagauucg gaacccggug guccuuagag cccucucuca ggcucgcaaa     1680

guuaucaaug gaguggugcg gagauauggc agccccgccc gcauucacau cgaaacagcu     1740

agagaggucg ggaaguccuu uaaggaucgg aaagagaucg aaaagcgcca agaggaaaac     1800

agaaaagacc gggagaaggc cgccgcuaaa uuccgcgaau acuuuccuaa uuucguggga     1860

gagcccaaga gcaaagauau ucugaagcug agacucuaug agcagcagca uggcaagugc     1920

cuguacucug ggaaagaaau caaccuuggc cggcugaaug agaagggaua uguugaaauu     1980

gaccacgcgc ucccuuuuuc ccgcaccugg gacgauagcu ucaacaauaa agugcugguc     2040

cugggcucug agaaccagaa uaaggggaac caaacacccu acgaauacuu uaauggaaaa     2100

gacaacagca gagaguggca ggaguucaag gcccgggugg aaaccucccg cuuuccgaga     2160

ucuaagaaac agcggauccu ucuccagaag uucgaugagg acggcuuuaa agaaagaaau     2220

cugaacgaua cgcgcuaugu uaaucgguuc cugugucaau uuguggcuga cagaaugcgc     2280

cucacaggga agggcaaaaa gcgggucuuc gccagcaacg gacagauuac caaccugcuu     2340

agaggguucu ggggccugcg caaggugcgg gccgagaaug auagacauca cgcucucgac     2400

gccgucgugg uugcgugcuc caccguggcu augcagcaga aaaucacacg cuuuguccgg     2460

uacaaggaga ugaacgccuu cgauggaaaa accauugaca aggaaacagg cgaggugcug     2520

caucagaaaa cgcacuuucc ucagcccugg gaguucuuug cccaggaagu uaugaucaga     2580

guguucggga agccugaugg aaagcccgaa uuugaggagg cugacacccu ggaaaaacuu     2640

cggacacucc uggccgagaa gcugagcucu cgcccugaag cgguccacga guaugugaca     2700

ccccucuucg uguccagagc uccgaaucgg aaaaugagcg gccaggggca uauggaaacc     2760

guuaagucug ccaaacgccu ggaugaggga gucuccgugc uuagagugcc ucugacccaa     2820

cucaagcuga aagaccugga gaagaugguc aaccgggaaa gagagcccaa acuuuacgaa     2880

gcccugaaag cucgccucga ggcccacaag gacgaucccg cuaaagccuu ugcggaaccu     2940

uucuauaagu acgacaaagc cggcaaucgg acgcaacagg ugaaggcugu uagaguggag     3000

caaguccaga agaccggggu guggguucgc aaccauaaug gcaucgccga uaacgcuaca     3060

auggugcggg ucgacguguu ugagaaagga gauaaguauu accugguucc gauuuacagc     3120

uggcaagugg ccaaaggcau ccuccccgac agagccgucg ugcaggggaa ggaugaagag     3180

gacuggcagc ugauugauga cuccuucaau uuuaaauucu cucuucaccc uaacgaucug     3240

guggaaguca ucaccaagaa ggcucgcaug uucggauauu uugcgagcug ucaccggggc     3300

acagggaaua uuaacaucag aauccaugac cucgaucaca aaauuggaaa gaauggcauc     3360

cuggagggga uuggcguuaa aaccgcccug uccuuccaga aguaccagau cgacgagcuu     3420

ggaaaagaaa uucgccccug cagacugaag aagcggccuc ccgugcgcaa aagaccugcu     3480

gccacgaaga aagccggcca ggcuaagaaa aagaaguaag gacuagugaa gggaacaccc     3540

aaauuuaauu cagccuuaag cacaauuaau uaagagugaa acguaauugu acaagcaguu     3600

ggucacccac cauagggcau gaucaacacc gcaaccuuuc cuuuuucccc cagugauucu     3660

gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa auuuaguaag cuuaaggcgg     3720

ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc ucucacuuuc aguaaauaaa     3780

auaaaagcag caacagaaau aaagaaauaa augaaauuca aaaugaaaua aauauugugu     3840

ugugcagcau uaaaaaauca auaaaaauua aaaaugagca agaucuaaaa aaaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu          3955


<210>  4673
<211>  4711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4673
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagaa accguacacc      240

auuggacugg acaucggcac aaacagcguu gggugggcgg ugcucacgga ucaguaugac      300

cuggugaagc ggaagaugaa aauugcugga gauuccgaga agaaacaaau caagaaaaau      360

uucuggggcg uccgccuuuu ugacgaaggg cagaccgccg ccgauagacg gauggcucgc      420

acagccagac ggcgcauuga gagacggcgc aacagaaucu cuuaccugca gggaaucuuc      480

gcugaagaga ugagcaagac cgacgccaau uucuucugcc ggcucuccga uucuuuuuau      540

guggacaacg aaaagcgcaa uagcagacau cccuucuuug cgacaauuga ggaggaaguu      600

gaguaccaca aaaacuaucc uaccaucuac caucugcggg aggaacuugu caauuccucu      660

gagaaggccg aucugcgccu cgucuaucug gcucuggccc acauuaucaa auacagaggc      720

aacuuccuca uugaaggcgc ucuggacaca cagaauacca gcguggaugg gaucuacaag      780

caauuuauuc agacguauaa ccagguuuuc gccuccggaa ucgaggacgg cagccuuaaa      840

aagcuggaag auaauaaaga cguggcgaag auccucgucg agaaggugac ccggaaagaa      900

aagcuggagc gcauucuuaa acuguacccc ggggagaagu cugccggaau guuugcucag      960

uucaucuccc ucauuguggg cagcaaaggg aacuuucaaa agccuuucga ccugaucgaa     1020

aagucugaua uugagugugc caaagacucc uaugaagagg aucuggaaag ccuccuggcu     1080

cuuaucggag acgaguacgc cgagcuguuc gucgccgcua agaaugcgua uucugccguu     1140

gugcucucca gcaucauuac aguggcugaa accgagacaa acgccaaacu guccgccucu     1200

augaucgaaa gauuugauac ccaugaggaa gaccuuggcg agcugaaggc uuucauuaaa     1260

cuccaucugc ccaagcacua cgaggaaauc uuuagcaaua cggagaagca cggcuaugcc     1320

ggguacauug auggaaaaac aaagcaggcg gacuucuaca aauauaugaa gaugacccug     1380

gaaaacaucg agggcgcuga uuacuuuauc gccaaaauug agaaggaaaa cuuccuccgg     1440

aagcagagaa cauuugacaa uggggccauc ccucaccagc ugcaucuuga ggaacuggag     1500

gcuauucucc accaacaggc caaauauuac ccguuccuga aggaaaacua ugauaaaauc     1560

aagucccuug ucaccuuucg cauucccuac uucgugggac cucuggcuaa uggccagagc     1620

gaguuugccu ggcucacccg gaaagcggac ggggagauca gacccuggaa cauugaagag     1680

aagguugacu ucggaaaauc ugccguggau uuuaucgaaa agaugacaaa uaaggacacg     1740

uaucugccua aagagaacgu ccugcccaag cacucccucu gcuaccaaaa auaccuggug     1800

uauaaugaac uuaccaaggu ucgcuacauc aacgaucagg gcaaaacaag cuauuucucu     1860

ggccaggaga aggagcagau uuucaaugac cuguuuaagc aaaaacggaa ggugaaaaag     1920

aaagaucucg aacuguuccu gagaaacaug agccaugucg aguccccgac caucgaaggg     1980

cuugaggacu cuuuuaauag cuccuacagc acauaucacg aucuccugaa ggugggaauu     2040

aagcaggaaa uccuggacaa cccugucaau accgagaugc ucgagaacau ugugaaaauc     2100

cugacgguuu ucgaagauaa gcgcaugauc aaagagcagc uucagcaauu uucugacgug     2160

cuggauggcg ucgugcucaa gaaacuggaa cggagacauu acacagggug gggacgccug     2220

uccgcuaagc uccucauggg cauucgggac aagcagagcc accugaccau ccuggauuau     2280

cucaugaaug acgacgggcu gaacagaaac cuuaugcagc ugauuaauga uucuaaccuc     2340

uccuucaaaa gcaucauuga gaaggagcag guuaccacag ccgacaaaga uauccaaucc     2400

aucguggcug accuggccgg cucucccgcg auuaagaaag gaauccugca gagccuuaag     2460

auugucgaug aacucguguc cgugaugggc uacccucccc agaccaucgu ugucgagaug     2520

gcccgggaaa aucagacaac ggggaaagga aagaacaauu cucgccccag auacaagagc     2580

cuggagaaag cuauuaagga auuuggcucc caaauccuga aagagcaucc uaccgacaac     2640

caggagcucc ggaauaacag acuguaucuu uacuaucugc agaaugggaa ggauauguac     2700

acaggacaag accucgauau ccacaaccug agcaauuaug acauugauca caucgugccg     2760

cagucuuuca uuaccgacaa cuccaucgau aaucuggugc uuacaagcuc ugccggcaac     2820

cgcgaaaaag gggacgacgu gcccccucuc gagauugugc ggaagagaaa gguguuuugg     2880

gaaaaacugu accagggcaa ucugaugucc aagcgcaaau ucgauuaucu caccaaggcu     2940

gagagaggag gccugacgga agccgacaaa gcccgguuua uccaucgcca gcuuguugag     3000

accagacaaa uuacaaagaa cgucgcuaac auccugcacc agcgguucaa uuacgagaag     3060

gaugaccaug ggaacaccau gaaacaggug cgcaucguua cacucaagag cgcgcuggug     3120

ucucaguuua gaaaacaauu ccagcuguac aagguccggg augugaauga cuaucaccac     3180

gcccaugaug cuuaccuuaa cggaguugug gccaauaccc ugcucaaagu cuauccccag     3240

cuggaaccug aguucgugua cggcgacuau caccaguuug auugguucaa ggccaacaag     3300

gcuacggcca aaaagcaauu uuacacaaau auuaugcuuu ucuuugcgca gaaagaccgc     3360

aucauugaug aaaacgggga gauccugugg gacaagaaau aucuugacac cgugaagaag     3420

gucaugagcu acagacagau gaauauuguu aaaaagacag aaauccaaaa aggagaguuc     3480

uccaaggcua ccaucaaacc caagggcaac agcucuaaac ugauuccgcg gaagaccaau     3540

ugggauccua ugaaguacgg gggccucgac ucccccaaca uggccuaugc cguggugauc     3600

gaguacgcua aaggaaagaa uaaacugguc uuugaaaaga aaauuauccg cgugacaauu     3660

auggagagaa aggccuucga aaaggaugag aaagcguuuc uggaggaaca gggcuaucgg     3720

cagccuaagg uucuugcuaa acugcccaag uacacgcucu augaguguga agaggggaga     3780

cgccggaugc uggccagcgc caacgaagcu cagaaaggaa aucaacaggu gcucccuaac     3840

caucugguca cccuucugca ccaugccgcu aacugcgagg uguccgacgg caagucucuc     3900

gauuacaucg agagcaauag agaaauguuc gccgagcugc uggcgcacgu uuccgaauuu     3960

gccaagcgcu auacacuugc ugaggccaac cugaauaaaa ucaaccagcu cuuugaacag     4020

aauaaggagg gggacauuaa agcuaucgcc caaucuuucg uggaucugau ggccuuuaac     4080

gcuaugggag cgcccgccag cuucaaguuu uucgagacca caauugaacg gaaaagauac     4140

aauaaccuca aggagcugcu uaauuccacc aucauuuacc agucuaucac gggccuguau     4200

gaaagccgca agcggcugga cgauaaaaga ccggcugcca caaagaaagc cgggcaggcu     4260

aagaaaaaga aauaaggacu agugaaggga acacccaaau uuaauucagc cuuaagcaca     4320

auuaauuaag agugaaacgu aauuguacaa gcaguugguc acccaccaua gggcaugauc     4380

aacaccgcaa ccuuuccuuu uucccccagu gauucugaaa aaccccucuu cccuucagcu     4440

ugcuuagaug uuccaaauuu aguaagcuua aggcggccua cagaagaaaa agaaaaaaaa     4500

ggccacaaaa guucccucuc acuuucagua aauaaaauaa aagcagcaac agaaauaaag     4560

aaauaaauga aauucaaaau gaaauaaaua uuguguugug cagcauuaaa aaaucaauaa     4620

aaauuaaaaa ugagcaagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                                    4711


<210>  4674
<211>  4744
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4674
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagaa accguacagc      240

auuggacugg acaucggcac caacuccguu gggugggcgg uggugacaga ugacuauaag      300

guccccgcua agaaaaugaa ggugcucgga aauacggaua aaucucauau ugagaagaac      360

cugcuuggcg cccuccuguu cgacagcggg aauaccgccg aagaucggcg ccugaaaaga      420

acagcucggc gcagauacac ccggcgcaga aaccggaucc uuuaucugca ggagauuuuc      480

uccgaagaga ugggaaaggu ugacgauucu uucuuucacc gccucgaaga cagcuuccug      540

gugacagagg auaagagagg cgagcggcau ccuaucuuug gcaaucugga agaggaaguc      600

aaauaccacg agaacuuccc caccaucuau caucuccgcc aauaccuggc cgacaauccu      660

gagaaggugg aucuuagacu gguuuaucuc gcucuggccc acauuaucaa auuucggggg      720

cacuuccuua uugaaggaaa guuugacaca cgcaacaaug augugcagag acuguuccag      780

gaguuucucg cggucuacga caacaccuuc gaaaauuccu cucugcagga gcaaaacguc      840

cagguggaag agauccugac ggacaaaauu agcaaguccg ccaaaaagga ucgggugcuc      900

aagcuguuuc ccaaugagaa aagcaacggc agauucgcug aauuucuuaa gcugaucguu      960

gggaaucagg ccgacuuuaa aaagcauuuc gagcucgaag agaaagcucc ucugcaguuu     1020

ucuaaggaua ccuacgaaga ggagcuugaa gugcugcucg cccaaauugg agacaacuau     1080

gcggagcugu uccuguccgc caagaaacuc uacgauagca uccugcuuuc uggcauccug     1140

acagugaccg acgucgggac aaaggcuccg cucuccgcca gcaugauuca gcgcuauaac     1200

gaacaccaga uggaucuggc ucagcuuaaa caauuuaucc ggcagaagcu gucugacaaa     1260

uacaaugagg uguucuccga uguuagcaag gacggauaug ccggcuacau ugauggcaag     1320

accaaccagg aagccuuuua uaaauaccuc aaggggcugc ugaauaaaau cgagggaucc     1380

ggcuacuucc ucgacaagau ugagagagaa gacuuucugc gcaaacaacg gacguucgau     1440

aacgggucua ucccccauca gauccaccuu caggagauga gagcuauuau ccgccggcag     1500

gcggaauuuu auccuuuccu ggccgacaau caagauagaa uugagaagcu ccugacauuu     1560

cgcauccccu acuauguggg accucuggcu cggggcaaga gcgacuucgc cuggcuuucc     1620

agaaaaagcg ccgauaagau uacccccugg aacuucgacg agaucgucga uaaagaaucu     1680

uccgcugagg ccuuuaucaa ucgcaugaca aacuacgacc ucuaucugcc gaaucagaag     1740

gugcugccua aacacagccu ccuguacgaa aaguucaccg uuuauaacga gcuuaccaaa     1800

gugaaguaca agacagaaca ggggaaaacg gcguuuuucg augcuaauau gaagcaggag     1860

auuuuugacg gagucuucaa aguguaccgg aaggucacca aagauaagcu gauggacuuu     1920

cucgagaagg aguucgauga auuuagaauc guggaccuga caggccugga caaagaaaac     1980

aagguuuuca augccucuua uggcaccuac caugaucuuu gcaaaauucu cgacaaggau     2040

uuucuggaca acagcaaaaa ugagaagauc cuggaagaua uugugcucac acugacccuu     2100

uucgaggacc gggagaugau ccgcaagaga cuggaaaacu auuccgaucu ccugacgaaa     2160

gagcaaguca agaaacugga acggagacac uacacagggu ggggacgccu uucugccgag     2220

cucauucaug gcauccggaa caaggaaagc agaaaaacca uccuggacua ucugauugau     2280

gacggaaauu ccaaccgcaa uuuuaugcag cucaucaacg augacgcucu gagcuucaag     2340

gaggagauug ccaaggcuca ggugaucggg gaaaccgaua aucuuaacca gguugugucu     2400

gacauugccg gcucccccgc gaucaaaaag gggauccugc aaagccucaa aauugucgac     2460

gagcugguga agaucauggg ccaccagccu gaaaauauug ugguugagau ggccagagag     2520

aaccaguuca caaaucaggg acggcgcaac ucucaacaga gacugaaagg ccuuaccgau     2580

uccaucaagg aauuugggag ccagauucuc aaagagcauc ccgucgaaaa uucccaacug     2640

cagaacgacc ggcuguuccu cuacuaucug cagaauggac gcgauaugua cacaggcgag     2700

gaacuugaca ucgauuaccu gucucaguau gacaucgauc acauuauccc ccaggcuuuu     2760

auuaaggaca acagcaucga uaauagagug cucacguccu cuaaggagaa ccgggggaaa     2820

agcgacgaug ugccuuccaa ggacgucgug cgcaaaauga aaagcuacug gucuaaacug     2880

cuguccgcca aacuuauuac ccaaagaaag uucgacaacc ugacaaaagc ugagcgggga     2940

ggccucaccg augacgauaa ggccggguuu aucaaacgcc agcugguuga aacaagacag     3000

aucaccaagc acguggcgcg gauucucgac gagagauuca auacggaaac cgaugagaac     3060

aauaaaaaga uccgccaagu caagauugug acacugaaaa gcaaccuugu uucuaauuuu     3120

cggaaggagu ucgaacugua uaaagugaga gagaucaacg acuaccauca cgcccaugau     3180

gcuuaucuca augccgucau uggcaaggcu cugcugggag uguacccgca gcuugaaccc     3240

gaguuuguuu auggcgacua cccucacuuc cacgggcaua aagaaaacaa ggccaccgcc     3300

aagaaauuuu ucuacuccaa uaucaugaac uucuuuaaga aagaugacgu gcgcacagau     3360

aagaauggag agauuaucug gaaaaaggac gaacacauca gcaacauuaa gaaaguccug     3420

ucuuaucccc aggugaauau cgugaagaaa gucgaggagc agaccggcgg guucagcaag     3480

gaauccauuc ucccuaaagg aaacagcgau aagcugaucc cccggaaaac gaagaaguuu     3540

uacugggaca caaagaagua uggcggguuc gacucuccga uuguugcuua cuccauccuc     3600

gugaucgcgg auauugagaa aggcaagagc aaaaagcuga agaccgugaa agcccuuguc     3660

ggagugacaa ucauggaaaa gaugaccuuu gagagagacc cuguugcuuu ccuggagcgc     3720

aaaggcuauc ggaaugugca agaagagaac auuaucaagc uccccaaaua cucccuguuu     3780

aagcuggaaa acgggagaaa gcgccuucug gccucugccc gggagcucca gaaaggaaau     3840

gaaauugucc ugccuaacca ucucggcacc cugcuuuauc acgcuaagaa uauccauaaa     3900

guggaugagc ccaagcaccu ggacuacguu gauaaacaca aggacgaguu caaggaacug     3960

cucgaugugg ucagcaacuu uuccaaaaag uacacacugg ccgaggggaa ucuugaaaag     4020

aucaaggagc uguaugcgca gaacaaugga gaagaccuca aagagcuggc uucuagcuuc     4080

auuaaccucc ugacguuuac cgccaucggc gccccugcua cauucaaguu uuucgauaaa     4140

aauauugaca gaaagcggua caccuccaca accgagaucc uuaacgccac gcugauucau     4200

caaucuauca cagggcugua ugaaacccgc auugaucuca auaagcuggg cggagacaaa     4260

agacccgcgg cuaccaagaa agccggccag gccaagaaaa agaaguaagg acuagugaag     4320

ggaacaccca aauuuaauuc agccuuaagc acaauuaauu aagagugaaa cguaauugua     4380

caagcaguug gucacccacc auagggcaug aucaacaccg caaccuuucc uuuuuccccc     4440

agugauucug aaaaaccccu cuucccuuca gcuugcuuag auguuccaaa uuuaguaagc     4500

uuaaggcggc cuacagaaga aaaagaaaaa aaaggccaca aaaguucccu cucacuuuca     4560

guaaauaaaa uaaaagcagc aacagaaaua aagaaauaaa ugaaauucaa aaugaaauaa     4620

auauuguguu gugcagcauu aaaaaaucaa uaaaaauuaa aaaugagcaa gaucuaaaaa     4680

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag     4740

aauu                                                                  4744


<210>  4675
<211>  3877
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4675
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcccagac aacaaaccug      240

agcuacauuc ucggacugga ccuuggcauc gcguccguug ggugggcugu gguggagauu      300

aaugaaaacg aggauccgau cggacugauu gacgucggcg ugcggaucuu cgaacgcgcc      360

gagguuccca agacggggga aucucucgcc cugagcagac ggcuugcucg cuccaccaga      420

cggcugaucc gcagacgggc ccaucgccuc cugcuggcua aaagauuucu caagcgggag      480

gggauucugu cuacaaucga ucuugagaag ggccugccua aucaagccug ggaacuccgc      540

guggcgggcc uggagagacg gcuuagcgcc auugaauggg gagcuguccu gcuccaccug      600

aucaaacauc gcggauaucu guccaagcgg aaaaacgagu cucagaccaa uaacaaggag      660

cucggcgccc ugcuuagcgg gguggcucag aaucaccagc ugcuccaauc cgacgauuac      720

agaacacccg ccgaacuggc gcuuaaaaag uucgccaagg aggaaggaca uauuagaaac      780

cagcgcggcg cuuauaccca cacauuuaau cggcuggacc uccuggccga gcugaaccuc      840

cuguucgcuc agcagcacca auuugggaau ccucauugca aagaacacau ccagcaguac      900

augaccgagc uucugaugug gcagaagccc gcccucagcg gagaggccau ucugaaaaug      960

cuuggcaagu guacgcauga aaagaacgag uucaaagcug cgaaacacac cuauucugcc     1020

gaaagauuug uuuggcugac aaagcucaau aaccugcgca uccuggagga uggcgcugaa     1080

cgggcccuca augaggagga aagacaacug cuuaucaacc acccuuacga gaaguccaaa     1140

cugaccuaug cccaggugcg caagcuccug gggcugagcg aacaggcuau uuucaaacau     1200

cuucgguacu cuaaggagaa cgccgaaucc gcgacauuua uggagcucaa ggcuuggcac     1260

gccaucagaa aagcccugga gaaucaagga cugaaggaca ccuggcagga ucucgcuaaa     1320

aagccggacc ugcuugauga aauuggcacg gccuucagcc uguacaaaac agacgaggau     1380

auccagcagu aucucaccaa caaggucccc aauucuguga uuaacgcucu gcuggugucc     1440

cuuaauuuug acaaauucau cgaacucagc cugaaguccc ugcgcaagau ccucccucug     1500

auggagcaag ggaaacggua cgaccaggcc ugcagagaga uuuauggaca ucacuacggc     1560

gaagcgaacc agaagacauc ucagcuucug cccgccaucc cugcucaaga gauucgcaau     1620

cccgucguuc uccggacccu gagccaggcc agaaaaguga ucaacgcuau uauccggcag     1680

uauggguccc cggcccgcgu gcauaucgaa accggaagag agcugggcaa gagcuuuaaa     1740

gaacggagag agauucagaa gcaacaggag gauaaucgca caaagcggga aucugcgguc     1800

cagaaauuca aggagcuuuu cuccgacuuu agcucugaac cuaaaagcaa ggauauccuc     1860

aaauucagac uguacgagca gcaacacggc aagugucugu auuccgggaa agaaauuaac     1920

auccaccgcc ucaaugagaa gggauacgug gagauugacc augcccugcc cuuuucuaga     1980

acgugggaug acagcuucaa caauaagguu cuugugcugg cuuccgaaaa ccagaauaaa     2040

ggcaaccaga ccccuuacga guggcuccaa gggaagauca acagcgaacg guggaaaaau     2100

uuugucgccc uggugcuggg aucucagugc uccgcugcca agaaacagcg ccuucucaca     2160

cagguuauug augacaacaa guucaucgau agaaaucuga acgacacccg guauaucgcc     2220

cgcuuucuga gcaauuacau ucaagagaac cuccugcuug ugggcaagaa uaaaaagaac     2280

gucuucacac ccaaugggca gaucaccgcu cugcucagau cucggugggg ccugauuaaa     2340

gcgcgcgaaa acaauaacag acaccaugcc cuggaugcua ucguggucgc cugugccacg     2400

cccuccaugc agcagaagau uacacgguuu auccgcuuca aagaggugca cccuuauaag     2460

aucgagaaua gauacgaaau gguugaccaa gagagcggag aaauuaucuc cccgcacuuu     2520

cccgagccuu gggcuuauuu ccggcaggag gugaacauua gagucuuuga caaucauccc     2580

gauaccgugc uuaaggaaau gcugccugac cgcccccagg ccaaccacca guucguucaa     2640

ccucucuucg ugucucgggc gccgaccaga aaaaugagcg gccaggggca uauggagaca     2700

aucaaguccg cuaaacgccu ggccgaagga auuucugucc uccggauccc ccugacccag     2760

cuuaagccua accugcucga gaauauggug aacaaagaaa gagagcccgc ccuguacgcu     2820

ggccugaagg cccgccuugc ggaguuuaau caagauccug cuaaggccuu cgccacaccc     2880

uuuuauaaac aagggggaca acaggugaag gcuauccggg uugaacaggu ccagaaaagc     2940

ggcgugcugg ugagagagaa caaugggguc gccgacaacg cuuccauugu gcgcacggau     3000

guuuucauca agaauaacaa auuuuuccuc gugccgauuu acaccuggca ggucgccaag     3060

ggcauccugc cuaauaaagc gauuguggcc cacaagaacg aagacgagug ggaagagaug     3120

gaugagggag cuaaguuuaa auucagccuc uuucccaaug accugguuga acuuaagacc     3180

aaaaaggagu acuucuuugg cuauuacauc gggcuggauc gggccacagg aaacaucucu     3240

cucaaagaac augacggcga gauuuccaag gggaaggaug gaguguauag agucggcgug     3300

aaacuggcuc ugagcuucga aaaguaccag guugacgagc uugggaaaaa ucggcagauc     3360

ugccgccccc agcagagaca gccugugcgg aagcgcccgg ccgccaccaa aaaggcuggc     3420

caagcgaaga aaaagaaaua aggacuagug aagggaacac ccaaauuuaa uucagccuua     3480

agcacaauua auuaagagug aaacguaauu guacaagcag uuggucaccc accauagggc     3540

augaucaaca ccgcaaccuu uccuuuuucc cccagugauu cugaaaaacc ccucuucccu     3600

ucagcuugcu uagauguucc aaauuuagua agcuuaaggc ggccuacaga agaaaaagaa     3660

aaaaaaggcc acaaaaguuc ccucucacuu ucaguaaaua aaauaaaagc agcaacagaa     3720

auaaagaaau aaaugaaauu caaaaugaaa uaaauauugu guugugcagc auuaaaaaau     3780

caauaaaaau uaaaaaugag caagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                              3877


<210>  4676
<211>  3955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4676
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgcggc uuucaagccg      240

aaccccauua auuacauccu gggacucgac auuggcaucg ccagcguugg gugggccaug      300

guggagauug augaagacga gaacccuauc ugccugaucg aucuuggagu gcgggucuuu      360

gaacgcgcug aggugcccaa aaccggcgac ucccuggcca uggcuagacg gcucgcccgc      420

ucuguuagac ggcugacacg cagacgggcg caucgccuuc ugagagcccg gcgccuccug      480

aagagagaag gggugcugca ggcugccgau uucgacgaga auggacucau uaagagccug      540

ccuaacacgc ccuggcaacu ucgggcugcc gcgcuggauc gcaaacucac cccucuggag      600

ugguccgccg uccuucugca ccucaucaag cauagaggcu aucugucuca gcggaaaaau      660

gaaggcgaga cagcugacaa ggaacugggg gcccuccuga aaggaguggc ugauaacgcc      720

cacgcccuuc agaccggcga cuuuagaaca ccggcugagc uggcgcucaa uaaguucgag      780

aaggaaagcg ggcauauucg caaccagcgg ggagauuacu cccacaccuu uucuagaaaa      840

gaccugcaag ccgagcuuau ccuccuguuc gaaaagcaga aagaguuugg caauccccac      900

guuagcgggg gacugaagga aggcauugag acacugcuca ugacccagcg cccugcucug      960

uccggcgaug ccgugcagaa aaugcuuggg cauuguacgu ucgagcccgc cgaaccuaag     1020

gcugccaaaa acaccuauac agcggagcgg uuuaucuggc ugaccaagcu caauaaccug     1080

cgcauucuug aacaaggaag cgagagaccc cugacagaca ccgaacgggc uacgcucaug     1140

gacgagccgu acagaaaguc uaaacugaca uaugcccagg cccgcaagcu gcucggccug     1200

gaggauaccg cuuucuuuaa agggcuucgg uacggaaagg acaaugccga ggcuuccaca     1260

cugauggaaa ugaaagccua ucacgcgauc agcagagccc ucgaaaagga gggccugaag     1320

gauaaaaagu cuccucugaa ccuuuccccc gaacuccagg acgagaucgg gaccgcuuuc     1380

agccuguuua aaaccgauga ggacauuaca ggacgccuga aggaucggau ccagccugaa     1440

auucucgagg cccugcuuaa acauaucucu uucgacaagu ucguccaaau uucccugaag     1500

gcucucagac ggaucgugcc ccugauggaa cagggcaaac gcuacgauga ggccugcgcg     1560

gagaucuacg gcgaccacua ugggaagaaa aauacggaag agaagauuua ccugcccccu     1620

aucccggccg augaaauuag aaaccccgug guccuucggg cucucagcca ggccagaaaa     1680

guuaucaacg gaguggugcg ccgguauggc uccccugcuc gcauucacau cgagaccgcc     1740

agagaagucg ggaagucuuu uaaggacaga aaagagaucg agaagcggca agaagagaau     1800

cgcaaagaca gagaaaaggc cgcugcgaaa uuccgggagu acuuucccaa cuucguggga     1860

gaaccuaaga gcaaagauau ucugaagcug cgccucuaug agcagcagca uggcaagugu     1920

cuguacuccg ggaaagagau caaucuuggc agacugaacg aaaagggaua uguugagauu     1980

gaccacgccc uccccuuuag ccggacaugg gaugacucuu ucaauaacaa agugcugguc     2040

cugggcuccg aaaaucagaa caaggggaau caaaccccgu acgaguacuu uaacggaaaa     2100

gauaauagcc gcgaauggca ggaguucaag gcuagagugg agacaucucg guuuccucgc     2160

agcaagaaac agagaauccu ucuccagaag uucgacgaag auggcuuuaa agagcggaac     2220

cugaaugaca ccagauaugu uaaccgcuuc cugugccaau uuguggccga ucggaugaga     2280

cucacgggga agggcaaaaa gcgcgucuuc gccuccaaug gacagauuac aaaccugcuu     2340

cggggguucu ggggccugag aaaggugcgc gcugaaaacg accggcauca cgcccucgau     2400

gcggucgugg uugcuuguuc uaccguggcc augcagcaga aaaucaccag auuuguccgc     2460

uacaaggaga ugaaugccuu cgacggaaaa acaauugaua aggagaccgg cgaagugcug     2520

caucagaaaa cacacuuucc ccagccuugg gaguucuuug cucaggaagu uaugauccgg     2580

guguucggga agcccgacgg aaagccugag uuugaagagg ccgacacgcc cgagaaacug     2640

agaacccuuc ucgcggaaaa gcugagcucc cggccggagg cuguccacga auaugugaca     2700

ccucuguucg ugagccgcgc ccccaacaga aaaaugucug gccaggggca uauggagacc     2760

guuaaguccg ccaaacggcu cgaugaagga gucagcgugc ugcgcgugcc ccuuacacaa     2820

cugaagcuca aagaccugga gaagaugguc aauagagagc gggaaccuaa gcuguacgag     2880

gcucuuaaag ccagacugga agcucacaag gaugacccgg ccaaagcguu ugccgagccc     2940

uucuauaagu acgauaaagc uggcaaccgc acccaacagg ugaaggccgu ucggguggaa     3000

caaguccaga agacgggggu guggguuaga aaucauaacg gcaucgcuga caaugccacc     3060

auggugcgcg ucgauguguu ugagaaagga gacaaguauu accucguucc uauuuacucu     3120

uggcaagugg ccaaaggcau ccugcccgau cgggcugucg ugcaggggaa ggacgaggaa     3180

gauuggcagc ucauugacga uuccuucaac uuuaaauuca gccugcaccc uaaugaccuu     3240

guggagguca ucacaaagaa ggcgagaaug uucggauauu uugccuccug ccaccgcggc     3300

accgggaaca uuaauauccg gauccaugac cuggaucaca aaauuggaaa gaacggcauc     3360

cucgaaggga uuggcguuaa aacagcucug ucuuuccaga aguaccagau cgacgagcug     3420

ggaaaagaga uuagacccug ucgccuuaag aagagaccuc ccgugcggaa acgcccggcc     3480

gccaccaaga aagcuggcca ggccaagaaa aagaaguaag gacuagugaa gggaacaccc     3540

aaauuuaauu cagccuuaag cacaauuaau uaagagugaa acguaauugu acaagcaguu     3600

ggucacccac cauagggcau gaucaacacc gcaaccuuuc cuuuuucccc cagugauucu     3660

gaaaaacccc ucuucccuuc agcuugcuua gauguuccaa auuuaguaag cuuaaggcgg     3720

ccuacagaag aaaaagaaaa aaaaggccac aaaaguuccc ucucacuuuc aguaaauaaa     3780

auaaaagcag caacagaaau aaagaaauaa augaaauuca aaaugaaaua aauauugugu     3840

ugugcagcau uaaaaaauca auaaaaauua aaaaugagca agaucuaaaa aaaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu          3955


<210>  4677
<211>  3961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4677
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagua ccauguugga      240

auugacgugg gcaccuucag cguggggcug gcggcuaucg aggucgauga cgccggaaug      300

ccgauuaaaa cacucucccu ggugucucac auccaugaua gcggccuuga ccccgaugaa      360

auuaaguccg ccguuacgcg gcuggcuucu agcgggaucg cccgcagaac ccggcgccuc      420

uauagacgga agcgcagacg gcugcagcaa cuugacaaau uuauccagcg ccagggaugg      480

ccugugauug agcuggaaga uuacuccgac ccccucuauc cuuggaaggu cagagcugag      540

cuggccgcgu cuuacaucgc cgaugaaaaa gagcggggcg agaagcugag cguggcucuc      600

cgccacauug ccagacaucg gggcuggcgc aaccccuaug cuaaaguuuc cagccuguac      660

cuuccugacg ggccgucuga ugccuucaag gcgaucagag aagagauuaa gcgggccucc      720

ggacagcccg ugccugaaac agcuaccguc ggccaaaugg ugacacugug cgagcucggg      780

acccugaaac uuagaggaga gggcggggug cugagcgccc gccuccagca gucugacuau      840

gcucgggaaa uccaggagau uuguagaaug caagaaaucg gacaggagcu guaccgcaag      900

aucauugaug ucguuuuugc cgccgaaucc cccaaaggca gcgcuucuuc ccgggugggc      960

aaggacccuc ugcagcccgg gaaaaauaga gcgcucaagg ccagcgacgc uuuccagcgc     1020

uaccggaucg ccgcccugau uggaaaccuu agagugcgcg ucgauggcga gaaacggauc     1080

cuguccgugg aggaaaagaa ucucguuuuu gaccaccugg ugaaccuuac accgaagaaa     1140

gagccugaau gggucaccau ugcugagauc cuggggaucg auagaggaca acucauuggc     1200

acggccacca ugacagacga uggggaacgc gcgggagcuc ggcccccuac ccacgacaca     1260

aauagaucua ucgugaacag ccggauugcu ccccugguug auugguggaa gaccgccucc     1320

gcccuggagc agcaugccau ggugaaagcu cucagcaaug ccgaggucga cgauuucgac     1380

ucucccgaag gcgcgaaggu gcaggccuuu uucgcugauc uggacgacga uguccacgcc     1440

aaacuugacu cccugcaucu cccugugggc cgcgcugccu auagcgagga uacgcugguu     1500

agacuuacac ggagaaugcu gucugacggg guggaucucu acaccgcgcg ccugcaagaa     1560

uuuggaaucg agccgagcug gacacccccu acaccccgga uuggcgaacc ugucgggaac     1620

cccgccgugg acagaguucu gaagaccgug ucccgcuggc ucgagucugc uaccaagacg     1680

uggggagccc cggagagagu caucaucgaa cacgugcggg agggcuucgu gaccgaaaaa     1740

cgcgcuagag agauggaugg ggacaugcgg cgcagagccg cccggaaugc uaagcuguuu     1800

caggagaugc aggaaaaacu uaacguucag ggcaagccua gccgcgcgga ucuguggaga     1860

uaucaauccg uccagcggca gaauugccag ugugccuacu gcggaagccc cauuacauuc     1920

ucuaacuccg agauggacca caucgugccu cgcgcuggcc aagggagcac caauacaaga     1980

gaaaaccucg uggccgucug ucaucggugc aaucagucua aaggaaacac ccccuuugcc     2040

auuugggcua agaacacguc caucgagggc gugagcguua aggaagccgu ggagagaaca     2100

cgccacuggg ucaccgauac cgggaugcgg uccacagacu ucaaaaaguu caccaaagcg     2160

gugguugaga gauuucagcg cgcuacaaug gaugaagaga uugacgcccg gucuauggaa     2220

agcguggccu ggauggcuaa ugagcugaga ucccgcgucg cccagcauuu cgcgucucac     2280

ggaacgaccg ugcggguuua uagaggcagc cugacagcug aagcccgccg ggccuccggg     2340

aucagcggca agcugaaauu uuucgacgga gugggcaagu cuagacucga ucggcgccau     2400

cacgcuauug acgccgcugu caucgccuuu accuccgauu acguggcgga gacacuggcc     2460

gugagaagca accugaagca aucucaggcu caccggcagg aggccccuca guggcgcgaa     2520

uuuaccggga aagacgcuga gcauagagcc gccuggcggg ucugguguca aaagauggaa     2580

aaacucuccg cucugcuuac ggaggaucug agagacgauc gcguuguggu gaugagcaau     2640

guccggcuca gacugggaaa cggcucugcg cacaaggaaa ccaucgggaa acugagcaag     2700

gugaaacuuu ccagccagcu cucuguuucc gacauugaua aggccagcuc cgaggcucug     2760

uggugcgccc ugacacgcga gcccggauuu gacccgaagg aaggccuccc ugccaauccc     2820

gagcggcaua ucagagugaa cgggacccac gucuaugcug gcgauaauau uggacuguuc     2880

cccgugucug ccggcagcau cgcgcuucgc gggggauacg cugaacuggg cuccucuuuu     2940

caccaugccc ggguuuacaa aauuacaagc gggaagaaac cugccuucgc uaugcucaga     3000

guguauacca ucgaccugcu gccguaccgc aaccaggauc uuuucuccgu cgagcucaag     3060

ccccaaacga ugucuaugag acaggccgag aaaaagcugc gggacgcgcu ggcuacagga     3120

aaugccgaau aucucggcug gcuggugguu gacgaugagc uuguggucga caccagcaag     3180

aucgccacag aucaggugaa agcuguggaa gccgagcugg ggaccauucg cagauggcgg     3240

gucgacggcu ucuuuucccc uagcaagcuc cgccugagac cccugcagau gucuaaagaa     3300

ggaaucaaga aagaguccgc uccugagcuu agcaagauua ucgaucggcc cggcuggcuc     3360

ccugccguua acaagcuguu cucugacggg aaugugaccg uggucagacg cgauucccug     3420

ggacggguga gacucgaaag cacagcgcac cuucccguua cguggaaagu gcaaaagcgc     3480

ccggccgcua ccaaaaaggc cggccaggcu aaaaagaaga aauaaggacu agugaaggga     3540

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa     3600

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu     3660

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua     3720

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua     3780

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     3840

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau     3960

u                                                                     3961


<210>  4678
<211>  3661
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4678
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgcgcg gauucuggcu      240

uucgacaucg gaauuagcuc caucggcugg gccuuuucug agaacgauga acucaaggac      300

ugcgggguuc gcauuuucac caaaguggag aauccgaaga caggagaaag ccuggcccuu      360

cccagacggc uggcucgcuc cgccagaaag cggcucgcuc gcagaaaagc ccggcugaac      420

caucuuaagc accugaucgc gaaugaguuu aaacucaacu acgaagauua ucagucuuuc      480

gacgagagcc uggccaaggc uuacaaaggc ucccugaucu cuccuuauga gcuccgcuuu      540

agagcccuga augaacuucu gagcaagcaa gauuucgcuc gggugauucu ccauaucgcc      600

aagcgcagag gguacgacga uauuaaaaac uccgacgaua aggagaaagg agcgauccug      660

aaggccauua aacagaauga agagaaacuu gcuaacuauc agagcgucgg cgaguaccug      720

uauaaagaau acuuucagaa guucaaggaa aauucuaaag aauuuacgaa cgugcggaau      780

aagaaagagu ccuacgaacg cuguaucgcc cagagcuucc ucaaggacga gcugaaacug      840

auuuuuaaga agcaaagaga guucggcuuu ucuuucucca aaaaguucga agaggaaguu      900

cucagcgugg cuuuuuauaa acgggcccug aaggauuucu cucaccuugu cgggaacugc      960

uccuuuuuca ccgacgagaa aagagccccc aagaauagcc cucuggcuuu uauguucgug     1020

gcgcucacac gcaucaucaa ccugcuuaau aaccugaaga acaccgaagg cauucucuac     1080

acaaaagaug accugaaugc ccugcucaac gagguucuga agaauggaac ccuuacauau     1140

aaacagacca agaaacugcu cgggcugucc gacgauuacg aguuuaaggg agaaaagggc     1200

acguauuuca ucgaguuuaa aaaguacaaa gaguucauua aggcucuugg ggagcauaac     1260

cugucucagg acgaucucaa ugaaaucgcc aaagacauua cccugaucaa ggaugagauc     1320

aaacugaaga aggcccucgc uaaauaugac cugaaccaaa aucagauuga uagccuuucc     1380

aagcuggagu uuaaagacca ccucaacauc agcuucaagg cccugaaacu ggugacaccc     1440

cuuaugcucg aaggaaagaa guacgaugag gcguguaaug aacugaaccu gaaagucgcu     1500

auuaaugagg acaagaaaga uuuucucccu gccuucaacg agaccuacua uaaggacgaa     1560

gugacaaauc cggugguccu gcgggccauc aaagaguaca gaaagguucu uaacgcucug     1620

cucaagaaau auggcaaggu gcacaaaauu aauaucgaac uggcccgcga ggugggcaag     1680

aaccauucuc agcgggcuaa aaucgaaaag gagcagaacg agaauuacaa ggccaaaaag     1740

gacgcggaac uggagugcga aaaacuuggg cucaagauua acuccaaaaa uauccugaag     1800

cugagacucu ucaaagagca aaaggaauuu ugugccuaua gcggagagaa gauuaaaauc     1860

ucugaucugc aggacgagaa gaugcuugaa auugaucaca ucuaccccua uagccgcucc     1920

uucgacgauu cuuacaugaa caaaguccug guguuuacca agcagaauca agagaaacuc     1980

aaccagacgc cuuucgaggc uuuuggcaau gacagcgcca aguggcagaa gauugaaguu     2040

cuggcuaaaa accugcccac aaagaaacag aagcggaucc uugauaaaaa uuacaaggac     2100

aaggaacaaa agaacuucaa ggauagaaau cucaacgaca cccgcuauau cgcccggcug     2160

gugcugaauu acacaaaaga uuaucucgac uuucugccuc uuuccgauga cgagaacacc     2220

aagcugaaug acacccagaa agggagcaag guccaugugg aggcgaaguc uggaaugcuc     2280

acauccgccc ugagacacac guggggcuuc agcgcuaaag aucgcaacaa ccaucugcac     2340

cacgccauug acgcuguuau cauugccuac gccaauaacu cuaucgugaa ggcuuuuucc     2400

gauuucaaaa aggaacagga gagcaauucc gcggaacuuu augccaaaaa gauuucugag     2460

cucgacuaca agaacaaacg gaaguuuuuc gagcccuuua gcggguucag acagaaaguc     2520

cuggauaaga ucgacgaaau cuucgugucc aaaccggagc ggaagaaacc uucuggagcu     2580

cugcaugaag agaccuuucg caaggaagag gaguucuauc aaagcuacgg cggcaaggaa     2640

gggguccuca aagcccugga gcuuggaaag auuagaaaag ugaauggcaa gaucguuaaa     2700

aacggggaua uguuucgggu ggacauuuuc aagcacaaga aaacaaauaa guuuuacgcc     2760

guccccaucu auaccaugga uuucgcucug aaagugcucc cuaacaaggc cguugcgaga     2820

uccaaaaagg gagaaauuaa ggacuggauc cugauggaug agaauuacga auuuugcuuc     2880

agccuguaua aagacucucu uauccugauu cagacaaagg auaugcagga gcccgaguuu     2940

guguacuaua acgcuuucac cuccagcacg gucucucuca ucguguccaa acaugacaau     3000

aaguuugaaa cacugagcaa aaaccagaag auucucuuca agaaugccaa cgagaaagaa     3060

gugaucgcca agucuauugg cauccaaaau cugaaaguuu ucgagaagua caucgucagc     3120

gcucuugggg aagugaccaa agccgaguuu cgccagcggg aggacuucaa gaaaaagaga     3180

cccgcggcua ccaagaaagc cggccaggcc aagaaaaaga aauaaggacu agugaaggga     3240

acacccaaau uuaauucagc cuuaagcaca auuaauuaag agugaaacgu aauuguacaa     3300

gcaguugguc acccaccaua gggcaugauc aacaccgcaa ccuuuccuuu uucccccagu     3360

gauucugaaa aaccccucuu cccuucagcu ugcuuagaug uuccaaauuu aguaagcuua     3420

aggcggccua cagaagaaaa agaaaaaaaa ggccacaaaa guucccucuc acuuucagua     3480

aauaaaauaa aagcagcaac agaaauaaag aaauaaauga aauucaaaau gaaauaaaua     3540

uuguguugug cagcauuaaa aaaucaauaa aaauuaaaaa ugagcaagau cuaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau     3660

u                                                                     3661


<210>  4679
<211>  4072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4679
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccagcga ccugguucuc      240

ggacuggaua uuggcaucgg guccguggga gugggcauuc uuaacaaggu caccggggag      300

aucauucaua aaaauucucg gaucuucccg gcggcucagg ccgaaaacaa ucuggugcgc      360

agaacaaacc ggcaaggacg cagacucgcc cggcgcaaga agcacagacg gguucgccug      420

aauagacuuu uugaggaaag cggccugauc acggacuuca ccaaaauuuc caucaaccuc      480

aaucccuacc agcugcgggu gaagggccug acagaugagc ucucuaacga ggagcuguuu      540

auugcucuua aaaauauggu caagcaucgc gggaucagcu aucuggacga ugccuccgac      600

gauggaaacu cuagcguggg cgacuacgcu cagauuguua aagaaaauuc caagcagcuc      660

gaaaccaaga caccugggca aauccagcug gagagauauc agaccuacgg acagcuucgg      720

ggcgauuuca caguggaaaa agacgggaag aaacaccgcc ugauuaacgu cuuucccacc      780

agcgccuaua gaucugaggc gcuccggauc cugcaaacgc agcaggaguu caauccucag      840

aucaccgaug aauuuauuaa cagauaccug gagauccuca caggaaagcg caaauauuac      900

cauggccccg gcaaugaaaa gucccggacc gacuacggga gauaucgcac aagcggagag      960

acccuggaua acauuuucgg cauccuuauu gggaaaugca cguuuuaccc ugacgaguuc     1020

cgggccgcua aggccucuua uacagcucaa gaauuuaacc ugcucaauga ccugaacaau     1080

cuuaccgugc cgacagagac caagaaacug uccaaggaac agaaaaacca gaucaucaau     1140

uacgugaaga acgagaaagc caugggaccc gcgaaacucu ucaaguauau ugccaaacug     1200

cugagcugug augucgcuga caucaagggc uacagaauug auaaaucugg gaaggccgaa     1260

auccacaccu uugaggcuua ucgcaaaaug aagacacucg aaacgcugga cauugagcaa     1320

auggaucggg agacccuuga caagcuggcc uacguucuca cacugaauac cgaaagagag     1380

ggaauccagg aagcccuuga gcacgaguuc gcugauggcu ccuucagcca gaaacaggug     1440

gacgaacugg ugcaauuucg caaggcgaac uccucuaucu ucggcaaagg guggcauaau     1500

uuuagcguca agcucaugau ggagcugauu ccugaacugu acgagacauc cgaggaacag     1560

augaccaucc ucacgcggcu gggaaaacag aagacaaccu cuucuuccaa caagaccaaa     1620

uauauugaug agaaacuucu gacagaagag aucuacaauc ccgugguugc caagagcgug     1680

agacaggcua uuaagaucgu caacgccgcc auuaaagaau auggcgacuu cgauaauauc     1740

gugaucgaga uggcucgcga aaccaacgag gacgacgaga agaaagccau ucaaaagauc     1800

cagaaggcga auaaagauga aaaggacgcu gccaugcuca aagccgcuaa ccaguacaac     1860

gggaaggccg agcugccuca cucuguuuuu cauggacaca aacagcuggc uacaaagauu     1920

cggcuuuggc accaacaggg cgaaagaugc cucuauacgg ggaagaccau cagcauucau     1980

gaucugauca auaacuccaa ucaguucgag guggaccaca uccugccccu cucuauuaca     2040

uuugaugaca gccuggccaa caaaguccuu guguacgcga ccgccaauca ggaaaagggc     2100

caacggacac cguaucaggc ucuggauucc auggacgaug ccuggagcuu ccgcgagcuc     2160

aaggcuuuug ucagagaguc uaagacccug uccaacaaaa agaaggaaua ccugcuuacg     2220

gaggaagaca ucagcaaauu cgaugugcgg aagaaauuua uugagagaaa ucucguugac     2280

acccgcuacg ccucucgggu gguccugaac gcgcugcagg aacauuucag agcccacaag     2340

aucgauacaa aaguguccgu ugugcgcgga caauuuacca gccagcucag acggcauugg     2400

ggcauugaga agacacgcga caccuaucac caccaugcug ucgacgcccu gaucaucgcu     2460

gccuccucuc agcuuaaucu guggaagaaa cagaagaaca cgcucgugag cuacuccgag     2520

gaucaacugc uggacauuga aacaggggag cuuaucucug augacgaaua uaaagagagc     2580

guguucaagg ccccuuacca gcacuucguu gauacccuca aauccaagga guuugaagac     2640

agcauucugu ucucuuauca ggucgauucc aaauuuaaua gaaagaucag cgacgcuaca     2700

auuuacgcga cccggcaggc caagguggga aaagauaagg cugacgagac cuaugugcug     2760

ggcaaaauca aggauaucua cacacaagac ggguacgaug ccuucaugaa aauuuauaag     2820

aaggacaaau cuaaguuucu cauguaccgc caugaccccc agacguucga aaaagucauc     2880

gagccuauuc uggaaaacua ucccaauaag cagaucaacg agaaaggaaa ggaggugccc     2940

uguaauccuu uucuuaagua caaagaggaa cacggcuaua uuagaaagua cuccaaaaag     3000

gggaacggcc cggaaaucaa aagccugaag uauuacgauu cuaagcucgg aaaccacauu     3060

gacaucaccc ccaaagauag caauaacaag guugugcugc aguccgucag cccuuggcgg     3120

gccgacgugu acuucaauaa aacaaccggc aaguaugaga uccuggggcu uaaauacgcu     3180

gaucuccaau uugaaaaggg aacaggcacc uauaagauuu cucaggagaa auacaacgac     3240

aucaagaaaa aggagggggu ugauuccgac agcgaguuca aauuuacgcu guauaagaau     3300

gaucugcucc uggugaaaga cacagaaacc aaggaacagc aacuuuuccg cuuucugucc     3360

agaaccaugc ccaagcagaa acauuacguc gagcucaagc cuuaugauaa acagaaguuc     3420

gaaggaggcg aggcccugau uaaagugcug gggaacguug cgaauagcgg ccagugcaag     3480

aagggacuug gcaaaagcaa caucuccauu uacaaggugc ggacagacgu ccuggggaau     3540

caacacauca ucaaaaacga gggagacaag cccaaacucg auuucaagcg cccggcugcc     3600

accaagaaag ccggccaggc uaagaaaaag aaauaaggac uagugaaggg aacacccaaa     3660

uuuaauucag ccuuaagcac aauuaauuaa gagugaaacg uaauuguaca agcaguuggu     3720

cacccaccau agggcaugau caacaccgca accuuuccuu uuucccccag ugauucugaa     3780

aaaccccucu ucccuucagc uugcuuagau guuccaaauu uaguaagcuu aaggcggccu     3840

acagaagaaa aagaaaaaaa aggccacaaa aguucccucu cacuuucagu aaauaaaaua     3900

aaagcagcaa cagaaauaaa gaaauaaaug aaauucaaaa ugaaauaaau auuguguugu     3960

gcagcauuaa aaaaucaaua aaaauuaaaa augagcaaga ucuaaaaaaa aaaaaaaaaa     4020

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu             4072


<210>  4680
<211>  4630
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4680
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaccca guucgaggga      240

uuuacaaacc uguaccaagu uagcaagacg cuccgguucg aacugauucc gcagggcaaa      300

acccuuaagc auauccagga gcagggguuu auugaagagg acaaggcgcg caaugaucac      360

uauaaagaac ugaagcccau cauugacaga aucuacaaaa cauaugcuga ucaaugccuc      420

cagcuggugc agcuugacug ggagaaccug uccgccgcca ucgauucuua ccggaaggag      480

aaaaccgaag agacacgcaa ugcucucauu gaagaacagg ccaccuauag aaacgcuauc      540

caugacuacu ucauuggacg gacagauaau cugaccgacg ccaucaacaa gcgccacgcg      600

gagauuuaua agggccuguu uaaagccgaa cucuucaaug ggaaggugcu gaaacaacuu      660

ggaacgguca ccacaaccga gcaugaaaac gcucugcuca gauccuuuga uaaguucaca      720

accuacuuua gcggcuucua cgagaaucgg aaaaacgugu uuucugccga agacaucagc      780

acggcuauuc cucaccgcau cguucaggau aauuucccca aguuuaaaga gaacugucac      840

aucuucacaa gacugauuac cgccgugccu ucccuucggg agcauuucga aaaugucaag      900

aaggcgaucg gcauuuuugu gucuacaagc aucgaggaag uuuucuccuu ucccuucuau      960

aaccagcugc ucacccagac ccaaauugac cuguacaauc agcugcucgg gggaaucagc     1020

cgcgaggccg gcacagagaa aaucaagggg cugaacgagg ugcuuaaccu ggcuauucag     1080

aaaaaugaug agacggccca caucauugcu ucucucccuc auagauuuau cccgcuguuc     1140

aagcaaauuc uuuccgaccg gaacacccug agcuuuaucc ucgaagaguu caaaucugac     1200

gaagagguca uccaguccuu uugcaaguau aagacacugc ugcgcaauga aaacgugcuc     1260

gagaccgccg aggcccuguu caaugaacuu aacagcauug aucugacaca caucuuuauu     1320

ucucacaaaa agcucgagac caucuccagc gcucugugug accauuggga uacgcuuaga     1380

aaugcgcugu acgaacggag aauuuccgag cucacaggaa aaaucaccaa gucugccaaa     1440

gagaaggugc agcgcagccu gaagcacgaa gacauuaacc ugcaggagau caucuccgcu     1500

gccggcaaag aacucagcga ggccuucaag cagaaaaccu cugaaauucu gucccaugcu     1560

cacgccgcgc uugaucaacc ccugccuaca acccucaaga aacaggagga gaaggaaauc     1620

cuuaagagcc agcuggacuc ucuucucggg cuguaucauc ugcucgauug guuugcuguc     1680

gacgagagca augaaguuga ucccgaguuc uccgcccggc ugacaggaau uaaacuugaa     1740

auggagccuu cucugagcuu cuacaacaag gccagaaauu augcuacgaa aaagcccuac     1800

uccguggaga aauuuaagcu caacuuccaa augccgaccc uggccagcgg cugggacgug     1860

aauaaagaaa agaacaaugg cgcuauccug uuugucaaga acgggcuuua cuaucucgga     1920

auuaugccua aacagaaggg ccgcuacaaa gcccugucuu ucgagcccac agaaaagacc     1980

uccgaggggu uugauaaaau guauuacgac uauuucccug acgcggccaa gaugaucccc     2040

aagugcagca cacagcugaa agcugugacc gcccacuuuc agacgcacac cacacccauc     2100

cuccugucua acaauuucau ugaaccucuu gagaucacca aggagauuua cgaucugaac     2160

aauccggaaa aagagcccaa gaaauuucaa acagcuuaug ccaagaagac cggagaccag     2220

aaaggcuacc gggaagcgcu cuguaagugg aucgauuuca cgagagacuu ucuguccaaa     2280

uacacaaaga ccacaagcau ugaucugucc ucucuucgcc cuagcuccca guauaaagac     2340

cucggggagu acuaugccga gcugaacccc cugcucuacc auaucucuuu ccagcggauc     2400

gcugaaaagg agauuaugga ugccguugaa accggaaagc uguaucuuuu ucaaaucuac     2460

aauaaagacu ucgcuaaggg ccaccauggc aaaccuaacc ugcacacccu cuauuggaca     2520

gggcuguuca gccccgagaa ucuggccaag acguccauua aacuuaacgg acaggccgaa     2580

cucuuuuaca gaccuaagag ccgcaugaaa cggauggcuc acagacuggg cgagaagaug     2640

cugaauaaga aacucaagga ucagaaaacc ccgauccccg acacacugua ccaagagcuu     2700

uaugauuacg ugaaccaucg ccugucucac gaccucuccg augaagcgcg ggcccugcug     2760

ccuaauguca uuaccaagga ggugagccau gaaaucauca aagacagacg guucacaucu     2820

gacaaguuuu ucuuucacgu ucccauuacc cuuaacuauc aggcugccaa uuccccuagc     2880

aaguucaacc agcgcgugaa ugccuaccug aaagagcauc ccgaaacgcc gaucauuggg     2940

aucgauagag gagagcggaa ccucauuuau aucacaguca uugacucuac cggcaagauc     3000

cuggagcaga gaagccucaa caccauccaa caguuugauu accagaaaaa gcuggacaau     3060

cgcgaaaaag agcggguggc ugccagacag gcgugguccg ucguggggac aauuaaggau     3120

cuuaagcaag gcuaucugag ccagguuauc cacgaaauug uggaccucau gauccacuac     3180

caggcugucg ugguucugga gaaccugaau uucggauuua aaucuaagcg caccggcauu     3240

gccgaaaaag ccguguacca gcaauucgag aagaugcuua ucgauaaacu gaacugccuc     3300

guccugaagg acuauccugc ugagaagguu gggggagugc ucaaucccua ccagcugaca     3360

gaucaguuua cguccuucgc caaaaugggc acccaaagcg gguuccuguu uuaugugccc     3420

gcgccuuaca cauccaagau cgacccgcug accggauucg ucgaucccuu uguguggaaa     3480

acaauuaaga accaugaauc uagaaaacac uuccucgagg gcuuugacuu ccugcauuau     3540

gaugugaaga ccggggacuu uauccugcac uucaagauga aucggaaccu uagcuuucag     3600

cgcggccugc cuggauucau gcccgcuugg gacauugucu uugagaaaaa ugaaacgcag     3660

uucgaugcca agggcacccc uuuuaucgcc gggaaaagaa uugugcccgu uaucgagaac     3720

caucgguuca caggacgcua cagagaccuc uauccugcua augaacugau cgcccuccug     3780

gaggaaaagg gcauuguguu ccgggauggg uccaacaucc uucccaaacu gcuggagaau     3840

gacgauucuc acgcuauuga caccaugguc gcccucaucc gcagcgugcu gcagaugaga     3900

aacuccaaug cggccacagg agaagauuac auuaacucuc cgguucggga ccuuaacggc     3960

guguguuuug auagccgcuu ccaaaauccu gaguggccca uggacgcuga ugccaacggg     4020

gcuuaccaca ucgcccugaa gggccagcuc cugcucaauc aucugaaaga guccaaggac     4080

cuuaagcugc agaacggaau uagcaaucag gacuggcugg ccuauaucca agaacucaga     4140

aacaaacggc cugcugcgac caagaaagcc ggccaggcua agaaaaagaa guaaggacua     4200

gugaagggaa cacccaaauu uaauucagcc uuaagcacaa uuaauuaaga gugaaacgua     4260

auuguacaag caguugguca cccaccauag ggcaugauca acaccgcaac cuuuccuuuu     4320

ucccccagug auucugaaaa accccucuuc ccuucagcuu gcuuagaugu uccaaauuua     4380

guaagcuuaa ggcggccuac agaagaaaaa gaaaaaaaag gccacaaaag uucccucuca     4440

cuuucaguaa auaaaauaaa agcagcaaca gaaauaaaga aauaaaugaa auucaaaaug     4500

aaauaaauau uguguugugc agcauuaaaa aaucaauaaa aauuaaaaau gagcaagauc     4560

uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaagaauu                                                            4630


<210>  4681
<211>  4609
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4681
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccagcau uuaccaggag      240

uucguuaaca aguauucccu gucuaaaacc cuccgguuug aacugauccc gcaaggaaag      300

acacuugaga auauuaaggc gcgcggccug auccucgacg augaaaagag agcuaaggac      360

uacaaaaagg ccaaacagau uaucgauaag uaucaucagu ucuuuaucga ggaaauucug      420

agcuccgugu gcaucucuga ggaccuucug cagaacuaca gcgaugugua uuucaagcuc      480

aaaaaguccg acgaugacaa ucugcaaaaa gauuuuaagu cugccaaaga cacgauuaag      540

aaacagauca gcgaguacau uaaggauucc gaaaaguuca aaaaccuguu uaaucagaac      600

cucaucgacg cuaagaaagg gcaggagagc gaucugauuc uuuggcugaa gcaaucuaaa      660

gacaauggaa ucgaacucuu caaggccaac uccgacauca ccgauauuga cgaggcucug      720

gagaucauua agagcuuuaa aggcuggaca accuauuuca agggguuuca cgaaaaucgg      780

aaaaacgucu acucuuccaa ugauaucccc acaagcauua ucuaccgcau cguggacgau      840

aaccuuccua aguuccugga gaauaaagcc aaguaugaau cucucaagga caaagcgccc      900

gaggccauua acuacgaaca gaucaagaaa gaucuggcug aggagcugac cuuugacauu      960

gauuauaaga cauccgaagu uaaucagaga guguucagcc ucgacgaggu cuucgaaauc     1020

gccaacuuua acaauuaccu gaaccagucc ggaauuacca aauucaauac gaucaucggc     1080

ggcaaguuug ugaacgggga gaauaccaag cggaaaggaa uuaacgaaua uaucaaucuu     1140

uacucucaac agauuaacga uaagacacug aaaaaguaua aaaugagcgu ucucuucaag     1200

cagauccugu ccgacaccga gagcaaaucu uuugugauug acaaacuuga ggaugacucc     1260

gaugucguga caaccaugca gagcuucuac gaacaaaucg cugccuuuaa gacgguggag     1320

gaaaaaucua uuaaggagac acugagccuc cuguucgacg aucugaaagc gcagaagcuc     1380

gaccugucca aaaucuacuu uaagaaugau aagucucuua ccgaccugag ccagcaaguc     1440

uucgaugacu auuccguuau cggcacagcc gugcucgaau acauuaccca gcagaucgcu     1500

ccuaaaaacc uggauaaucc cagcaagaaa gagcaggagc uuauugccaa gaaaaccgaa     1560

aaggcuaagu aucugucucu cgagacaauc aaacuggccc uggaagaguu uaacaagcau     1620

cgcgacauug auaaacaaug uagauucgag gaaauccucg ccaauuuugc ugcgaucccu     1680

augauuuucg acgagaucgc ccagaacaag gacaaucugg cucagauuuc caucaaauac     1740

cagaaccaag ggaagaagga ucuucugcag gccagcgccg aagacgaugu gaaagcuauu     1800

aaggaccucc uggaucagac gaacaaucug cuucacaaac ucaagaucuu ccauaucucu     1860

caguccgagg acaaagccaa cauucuggau aaggacgaac acuuuuaucu ggucuucgag     1920

gagugcuacu uugaacucgc gaauaucgug ccgcuguaua acaaaauucg gaauuacauc     1980

acccaaaagc ccuacagcga ugagaaguuc aaacuuaacu uugaaaauuc cacacuggcu     2040

aacggauggg acaagaauaa agagccugau aacaccgcca uucucuucau caaggacgau     2100

aaauauuacc ugggcguuau gaauaagaag aacaauaaaa ucuuugacga caaggccauu     2160

aaagaaaaca agggggaggg auauaaaaag aucguguaca agcugcuucc cggcgcuaau     2220

aaaaugcucc cuaaggucuu cuuuucugcc aaaagcauua aguucuauaa ccccuccgag     2280

gauauccugc gcauuagaaa ccacucuaca cauaccaaaa auggcagccc gcagaagggg     2340

uacgaaaagu uugaguucaa caucgaagac ugucggaaau uuauugauuu cuauaagcag     2400

uccaucagca aacacccuga guggaaggac uucggauuuc gcuucucuga uacgcaaaga     2460

uacaauucca ucgacgaauu uuaccgggag guggagaacc agggcuauaa acugacauuc     2520

gaaaauauua gcgagucuua caucgauucc guugugaacc aggggaagcu cuaucuguuu     2580

cagauuuaca auaaggacuu cagcgcuuau ucuaaaggac gccccaaccu ucauacccug     2640

uacuggaagg cccucuuuga ugaaagaaau cugcaagacg ucguguauaa acugaacggc     2700

gaggcggagc uuuucuaccg gaagcagagc aucccuaaaa agauuaccca ccccgccaaa     2760

gaagcuaucg ccaauaagaa caaggauaau cccaaaaagg aguccgucuu ugaauacgac     2820

cucaucaaag acaagagauu cacagaggau aaauuuuucu uccacugccc uauuaccauc     2880

aacuuuaaga gcucuggggc uaauaaguuc aacgacgaaa uuaaccugcu gcucaaagag     2940

aaggccaaug augugcauau ccuguccauu gaccgcggag agcggcaccu ugcguauuac     3000

acacugguug auggcaaagg caacaucauc aagcaggaca cguuuaauau uaucgggaac     3060

gauagaauga aaaccaauua ucaugacaag cucgccgcua uugaaaagga ucgcgacagc     3120

gcccggaaag auuggaagaa aaucaacaau auuaaggaga ugaaagaagg auaccugucc     3180

cagguggucc acgagaucgc uaagcuggug auugaauaua acgccaucgu uguguucgag     3240

gaccuuaauu uuggcuucaa gagagggcgc uuuaaagucg agaagcaagu guaccagaaa     3300

cucgaaaaga ugcugaucga gaaacugaac uaucucgugu ucaaggauaa ugaauuugac     3360

aaaacaggag gcguucugcg ggccuaccag cuuaccgcuc cguucgagac auuuaagaag     3420

auggggaaac agaccggcau uaucuacuau guccccgcgg gauucacguc uaagauuugu     3480

ccugugaccg gcuuugugaa ccaacuguac cccaaauaug aaagcgucuc caagucucag     3540

gaguucuuca gcaaauuuga caagaucugc uacaaucucg auaaggggua uuucgaguuu     3600

uccuucgacu acaaaaacuu uggagauaag gccgcuaaag gcaaguggac aauugccucu     3660

uucgggagca gacugaucaa uuuucgcaac uccgacaaaa aucauaacug ggauacccgg     3720

gaaguguauc cuacaaagga gcuggaaaaa cuucugaaag acuacagcau cgaguacgga     3780

cacggcgagu guauuaaggc cgcuaucugc ggggaaucug auaaaaaguu cuuugccaaa     3840

cucaccuccg uucugaacac gauucuccag augagaaaua gcaagacagg caccgagcug     3900

gacuaucuua ucucucccgu ggcggauguc aacggaaauu ucuuugacuc ccgccaagcu     3960

ccgaagaaca ugccucagga ugccgacgcc aauggcgcuu accacauugg gcugaaagga     4020

cucaugcugc ugggccggau caagaacaau caggaaggga aaaagcugaa ccuugugauc     4080

aaaaaugagg aauauuucga guuuguucag aacagaaaua acaagcggcc cgccgcgaca     4140

aagaaggcug gacaagccaa gaaaaagaaa uaaggacuag ugaagggaac acccaaauuu     4200

aauucagccu uaagcacaau uaauuaagag ugaaacguaa uuguacaagc aguuggucac     4260

ccaccauagg gcaugaucaa caccgcaacc uuuccuuuuu cccccaguga uucugaaaaa     4320

ccccucuucc cuucagcuug cuuagauguu ccaaauuuag uaagcuuaag gcggccuaca     4380

gaagaaaaag aaaaaaaagg ccacaaaagu ucccucucac uuucaguaaa uaaaauaaaa     4440

gcagcaacag aaauaaagaa auaaaugaaa uucaaaauga aauaaauauu guguugugca     4500

gcauuaaaaa aucaauaaaa auuaaaaaug agcaagaucu aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                 4609


<210>  4682
<211>  4497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4682
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc cgacaagaaa uacagcauug gacuggauau cggcaccaac      180

uccguugggu gggcggugau uacagacgag uauaaggugc cgucuaagaa auucaagguc      240

cucggaaaua cggaucggca uagcaucaaa aagaaccuga uuggcgcucu gcuguuugac      300

uccggggaaa ccgccgaggc cacacgccuc aaaagaaccg cucggcgcag auacacacgg      360

cgcaagaaua gaaucugcua ucugcaggaa aucuucucua acgagauggc caagguggau      420

gacagcuuuu uccaccggcu ugaagagucc uuucugguug aggaagauaa aaagcaugag      480

cgccacccca uuuucggaaa uaucguggac gaagucgcuu accaugagaa auauccuacc      540

auuuaccacc ucagaaagaa acugguggau ucuacagaca aggccgaucu gcggcucauc      600

uaucuggcgc uugcccacau gauuaaauuu cgcggccauu uccugaucga gggcgaccuc      660

aaccccgaua auagcgacgu ugacaagcug uuuauucaac uugugcagac cuacaaccag      720

cuguucgaag agaauccuau caacgcuucc ggggucgaug ccaaggcuau ccucagcgcc      780

agacugucua aaucccggcg ccuggaaaau cucauugcgc agcugcccgg agagaagaaa      840

aacggccuuu uugggaaucu gaucgcccuc agccugggac uuacgccuaa cuucaagucu      900

aauuuugacc uggcugaaga ugccaaacuc caacugucca aggacaccua cgaugacgau      960

cuggacaacc uccuggcuca gauuggcgau caguaugccg accuuuuccu ggccgcuaag     1020

aaccucagcg augcgauccu gcuuucugac auucugagag ugaauacaga gaucaccaaa     1080

gccccgcucu ccgcuagcau gaucaagcgg uacgacgagc accaucagga ucugacacug     1140

cucaaagccc uggugagaca acagcuuccc gaaaaguaua aagagauuuu cuuugaccag     1200

uccaagaacg gguacgccgg auauaucgau ggcggcgcuu cucaggaaga guucuacaag     1260

uuuauuaaac cuauccugga aaagauggac gggaccgagg agcuccuggu caaacugaau     1320

cgcgaagauc uucuccggaa gcaaagaacg uucgacaacg gaagcauucc ccaccagauc     1380

caccugggcg agcugcaugc cauccuccgc cggcaggaag auuuuuaucc uuuccugaaa     1440

gacaauagag agaagauuga aaagauccuu acauuucgca uucccuacua cguugggccg     1500

cuggcgcggg gaaacuccag auucgcuugg augacccgca aaagcgagga gacaaucacc     1560

ccuuggaauu uugaagaggu gguggauaag ggcgccucug cccaauccuu cauugaacgg     1620

augaccaacu uugacaaaaa ucuccccaac gagaaggucc ugccuaaaca cagccugcuu     1680

uaugaguacu ucacagugua uaaugaacuc acgaagguua aauacgugac cgaggggaug     1740

agaaagcccg cuuuucuguc uggagaacag aagaaagcca ucgucgaucu ucucuucaag     1800

acaaaccgca aagugaccgu uaagcagcug aaagaggacu auuucaagaa gauugaaugu     1860

uuugauagcg uggagaucuc cggcgucgag gaccgguuca augcuucucu uggcacauac     1920

caugaccugc ucaaaaucau uaaggauaaa gacuuucugg auaacgaaga gaaugaagac     1980

auccuggagg auauugugcu uacccucacg cuguucgaag acagagagau gaucgaggaa     2040

cggcugaaga cauaugccca ccucuuugau gacaaaguca ugaagcagcu gaagcgcaga     2100

cgguacaccg gguggggaag acuuagccgc aaacugauua acggcauccg ggauaagcaa     2160

uccgggaaaa ccauccucga cuuccuuaag agcgauggau uugcgaacag aaauuucaug     2220

cagcugauuc augacgauuc ucugacauuu aaagaggaca uccagaaggc ccaggugucc     2280

ggccaagggg acagccucca cgaacacauu gcuaaccugg ccggcucucc cgcuaucaag     2340

aaaggcauuc ugcagaccgu uaaggugguc gaugagcucg ugaaaguuau gggacgccau     2400

aagccugaaa auaucgugau cgagauggcc agagagaacc agacaacgca gaaagggcaa     2460

aagaacuccc gggaacgcau gaaaagaauu gaggaaggaa ucaaggagcu gggcagccag     2520

auucuuaagg agcacccggu cgaaaauacc cagcugcaga augagaaacu cuaccuguau     2580

uaccugcaaa acgggcggga cauguaugug gaucaggaac uugacaucaa ucgccugucc     2640

gauuacgacg uggaucauau uguuccccag ucuuuccuca aggacgauag caucgacaac     2700

aaaguccuga caagauccga uaagaaucgg ggaaaaucug acaacgugcc uagcgaggaa     2760

guggucaaga agaugaaaaa uuauuggcgc caacuccuga acgcgaaacu uaucacccag     2820

agaaaauuug acaaucugac aaaggccgag cggggcgggc ucuccgagcu ggauaaagcu     2880

ggcuucauua agcgccagcu gguggaaacc agacagauca cgaagcacgu ugcccaaauu     2940

cuugacagcc ggaugaacac caaauacgau gagaacgaca agcugaucag agaagugaaa     3000

gucauuacac ucaagucuaa acuggugucc gauuuucgca aggacuucca guucuauaag     3060

guucgggaga ucaauaacua ccaccaugcu cacgaugccu accucaaugc cguggucgga     3120

accgcucuga uuaaaaagua ucccaaacuu gaaagcgagu uuguguacgg cgacuauaag     3180

guuuacgaug ugagaaaaau gaucgcgaag ucugagcagg aaaucgggaa ggccacagcu     3240

aaauauuucu uuuacuccaa cauuaugaau uucuuuaaga ccgagaucac gcuggccaac     3300

ggagaaauuc gcaaacggcc ucucaucgag acaaauggcg aaaccgggga gauugucugg     3360

gacaagggaa gagauuucgc cacagugcgc aaagugcuga gcaugcccca ggucaacauc     3420

guuaagaaaa ccgaggugca aaccggcggg uuuucuaagg aaagcauccu gccgaagaga     3480

aauuccgaca aacuuauugc ucggaagaaa gauugggacc cuaagaaaua uggcggauuc     3540

gacagcccca caguggccua cucuguccug gugguugcga agguggagaa gggcaaaucc     3600

aagaaacuca agagcgucaa agaacugcuc gggaucacga uuauggagcg cuccucuuuu     3660

gagaagaacc cuaucgauuu ccuggaagcu aagggauaca aagaggugaa gaaagaccuu     3720

auuaucaagc ugcccaaaua uagccuguuu gaacucgaga auggccggaa gagaaugcug     3780

gccuccgccg gggaacuuca gaagggaaac gagcuggcuc ucccuucuaa auacguuaau     3840

uuccuguauc ucgccagcca uuacgagaag cugaaaggcu cccccgaaga uaacgagcag     3900

aagcagcuuu ucguggaaca acacaaacau uaucuggacg agaucauuga acagaucucu     3960

gaguuuagca agcgggucau ucuggcggau gcuaaucucg acaaagugcu guccgccuac     4020

aacaagcacc gcgauaagcc gaucagagag caggccgaaa acauuaucca ccuuuucacc     4080

cugacaaauc ucggggcucc ugccgcuuuu aaauauuucg acaccacaau ugaucggaag     4140

cgcuacacca gcacgaaaga gguucuggac gccacacuca uccaucaauc uaucaccggc     4200

cuguacgaaa ccagaauuga ucuuucccag cugggaggcg acaagcggcc cgcggccaca     4260

aagaaggcug ggcaggccaa gaaaaagaaa uaaggacuag uaauaaggaa acuguaugaa     4320

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4380

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4497


<210>  4683
<211>  5280
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4683
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc caacuucaag auucugccga ucgcgauuga ccucggaguu      180

aaaaauacag gcguguuuag cgcuuucuac cagaagggga caucccugga gcggcuugau      240

aacaagaaug gaaaagugua ugaacugucu aaggacagcu acacgcuccu gaugaacaau      300

cgcaccgcca gacggcauca acgcagaggc aucgaucgga aacagcuugu caagcgccug      360

uuuaaacuca uuuggacaga gcagcugaac cuggaauggg acaaggauac ccagcaagcc      420

aucuccuucc ucuuuaauag acggggguuc ucuuuuauca cagacggaua uagccccgag      480

uaccugaaca uugugccuga acagguuaag gcuauccuua uggauauuuu cgacgauuau      540

aauggcgagg acgaucugga cuccuaccuc aaacuggcca ccgagcagga aucuaagauc      600

agcgagauuu auaacaaacu uaugcagaag auccuggaau uuaaacucau gaagcugugc      660

acagauauua aagacgacaa gguguccacc aagacgcuga aagagaucac cagcuacgag      720

uucgaacucc uggcugauua ccuugccaau uauucugagu cccugaagac acaaaaauuu      780

agcuacaccg acaagcaggg caaccucaaa gaacugucuu auuaccacca ugauaaguau      840

aauauccagg aguuccuuaa gcgccacgcg acaauuaacg acagaauccu ggauacccuc      900

cugacggacg aucuggacau uuggaauuuu aacuucgaaa aauucgauuu ugacaagaac      960

gaggagaaac uccagaauca agaagauaag gaccauaucc aggcccaucu gcaccacuuc     1020

gucuuugcug ugaacaaaau uaaguccgag auggccagcg ggggacggca ccgcucucag     1080

uacuuccaag aaaucacaaa uguucuugac gagaacaauc aucaggaagg cuaucugaag     1140

aacuuuugug agaaucucca caacaaaaag uacuccaauc ugagcgugaa aaaccuuguc     1200

aaucugaucg ggaaccucuc caaucuggag cugaagcccc ucagaaaaua cuucaacgau     1260

aagauucacg cuaaggccga ccauugggau gaacagaaau uuaccgagac auauugccac     1320

uggauccugg gagaauggcg ggugggcgug aaggaccagg auaaaaagga cggggcgaaa     1380

uacucuuaua aggaucuuug uaaugagcug aaacaaaagg ucaccaaggc cggacucguu     1440

gacuuccugc uggaacuuga uccuugccgc accauucccc cuuaccucga caacaacaau     1500

agaaaaccgc ccaaguguca gagccugauc cugaacccua aauuucucga uaaucaguau     1560

cccaacuggc agcaauaccu gcaggagcuu aagaaacugc aguccauuca gaauuaucuc     1620

gacagcuucg agacagaucu gaaggugcug aagucuucca aagaccaacc uuacuuugug     1680

gaauacaaga gcucuaacca gcagaucgcu agcggccagc gggacuauaa agaucuugac     1740

gccagaaucc uccaauucau uuuugaucgc gucaaggcuu ccgacgagcu gcugcucaau     1800

gaaaucuacu uccaggccaa aaagcugaag cagaaagccu cuagcgagcu ugagaagcug     1860

gaauccagca aaaagcucga ugaggugauu gcuaacucuc aacuguccca gauccugaaa     1920

agccagcaua cgaauggcau uuucgaacag gggaccuuuc uucaccucgu uugcaaguau     1980

uacaagcaac ggcagagagc gcgcgacucu cggcuguaua ucaugcccga guacagauau     2040

gauaaaaagc ugcauaaaua caacaauaca ggacgcuucg acgaugacaa ccagcuccug     2100

accuacugua aucacaagcc gcggcagaaa agauaucaac uucugaacga ucucgccggc     2160

gugcugcagg ucuccccuaa uuuucugaag gacaaaauug ggagcgauga cgaccuuuuc     2220

aucuccaagu ggcucgugga acacauccgc ggauuuaaga aggcuugcga ggauucucug     2280

aaaauucaga aagacaaccg gggccugcuc aaccauaaga ucaauauugc cagaaacaca     2340

aaagggaagu gugagaagga aaucuucaau cugauuugca aaaucgaggg aagcgaagau     2400

aagaaaggca acuacaagca cggccuugcc uaugagcugg ggguucuccu guuuggagaa     2460

cccaaugagg cuuccaaacc ugaguucgac cgcaagauca agaaauuuaa cucuauuuac     2520

agcuucgccc agauccaaca gauugcguuu gcugaacgga agggcaaugc caacaccugu     2580

gccgugugcu ccgcugauaa ugcccauaga augcagcaaa ucaaaauuac ggagcccguc     2640

gaagacaaca aggauaaaau cauccugagc gcuaaggccc agcggcuucc cgcgauuccu     2700

acacgcaucg uggacggggc cgucaagaaa auggcuacca uucuggccaa gaauaucgug     2760

gaugacaacu ggcagaauau uaaacagguu cucucugcua agcaccaacu gcacaucccg     2820

aucauuaccg aguccaacgc cuucgaauuu gagcccgcgc ucgccgaugu gaaaggaaag     2880

agccugaagg acagacggaa aaaggcucuu gagagaaucu cuccugaaaa uauuuucaaa     2940

gacaagaaca accgcaucaa agaguucgcc aagggcauuu ccgcuuauag cggggccaau     3000

cugacagaug gcgacuuuga uggagccaaa gaggaacucg accauaucau uccccggucu     3060

cacaagaagu acggcacccu gaacgaugag gcuaaucuga ucugcgucac aagaggggac     3120

aacaaaaaua agggaaaccg caucuucugu cuuagagauc uggcggacaa uuauaaacuc     3180

aagcaguuug aaacgaccga ugaccuggag auugagaaaa agaucgccga uacaauuugg     3240

gacgcuaaca agaaagauuu caaguuuggc aauuaccgga gcuucaucaa ccucaccccu     3300

caggaacaga aagccuuucg ccaugcccug uuccuugcug acgaaaaucc cauuaagcaa     3360

gccgugauca gagcgaucaa caaucggaac cgcacauuug uuaaugggac ccagagauac     3420

uucgcugagg ugcuggccaa caauauuuau cuccgggcca aaaaggagaa ccugaacacg     3480

gacaagaucu ccuuugauua cuucggcauu ccgaccaucg gaaaugggcg cggcauugcu     3540

gaaaucagac agcuguauga gaaagucgac agcgauaucc aggccuacgc gaagggagac     3600

aaaccucagg cuucuuauuc ccaccuuauu gaugccaugc uggccuucug caucgcugcc     3660

gacgaacauc ggaacgaugg cagcauuggg cucgagaucg acaagaauua cucccuguau     3720

ccccucgaua aaaacacagg agaaguguuu accaaggaca uuuucucuca aaucaagauu     3780

acagauaaug aguuuagcga caaaaagcug gugcgcaaaa aggcuaucga gggcuucaac     3840

acccacagac agaugacgcg ggaugggauc uacgccgaaa auuaccuucc uauucugauc     3900

cacaaagagc ugaacgaagu uagaaaggga uauacaugga aaaauuccga ggaaauuaag     3960

aucuuuaagg gcaaaaagua cgacauucag caacucaaca aucuggucua uugucuuaaa     4020

uucguggaca agcccaucuc uaucgauauu cagaucagca cccuggagga gcuccgcaac     4080

auucugacaa ccaauaacau cgcggccacc gcugaauacu auuacauuaa ucucaaaaca     4140

cagaagcugc augaguauua caucgaaaac uacaacacgg cccuugggua uaagaaauac     4200

uccaaggaga uggaguuucu gcggucucug gcuuauagaa gcgaacgcgu gaaaaucaag     4260

uccauugacg augucaaaca agugcucgac aaggauagca auuucaucau uggcaagauc     4320

acccugccuu uuaaaaagga guggcagcgg cuuuacagag aauggcagaa cacaaccauu     4380

aaagacgauu augaguuccu gaagucuuuc uucaauguua aauccaucac aaagcuccac     4440

aagaaagugc gcaaggacuu uagccugccc auuucuacca acgaaggaaa auuccucguc     4500

aagcggaaaa cgugggauaa uaacuucauc uaccagaucc ugaaugacuc cgauagcaga     4560

gccgacggca caaagccguu uauuccugcc uucgacaucu cuaaaaacga gauuguggag     4620

gcuaucauug auuccuuuac cagcaagaau aucuucuggc uucccaagaa caucgaacug     4680

caaaaaguug acaauaagaa cauuuuugcg aucgauaccu ccaaaugguu cgagguggaa     4740

acacccagcg accugcgcga uauugggauc gccaccauuc aguauaagau cgacaauaac     4800

ucucggccua aagucagagu gaagcucgau uacguuaucg acgaugacag caagauuaau     4860

uacuuuauga accauucccu gcuuaaaucu cgguauccgg auaaggugcu ggagauccuc     4920

aaacagagca caauuaucga auuugagucc ucuggauuua acaagacgau uaaagagaug     4980

cugggcauga agcucgcugg gaucuacaau gaaaccagca acaauaagcg ccccgccgcc     5040

accaaaaagg cuggacaggc caaaaagaaa aaguaaggac uaguaauaag gaaacuguau     5100

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     5160

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     5220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     5280


<210>  4684
<211>  3552
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4684
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc caagcggaac uacauucugg gacucgacau cggcauuacc      180

agcguugggu auggaaucau ugauuacgag acacgcgacg ugaucgaugc gggcgugaga      240

cuguucaaag aagcuaaugu cgagaacaau gaagggcggc gcuccaagag aggagcccgg      300

cgccuuaaga gacggcgcag acaucggauc cagcgcguga aaaagcugcu cuuugacuau      360

aaccugcuua cggaucacuc ugagcugagc ggcauuaauc cguacgaagc cagaguuaaa      420

ggccucuccc aaaagcuguc ugaggaggag uucagcgcug cccugcucca ucuggcuaaa      480

cggcgcgggg ugcacaacgu caaugaagug gaagaggaca ccggaaacga gcuuuccaca      540

aaggaacaga ucucuagaaa uagcaaggcc cuggaggaaa aauauguugc ggagcuccag      600

cuggaacggc uuaagaaaga uggcgaggug agagggucca uuaaccgcuu uaagaccagc      660

gacuacguca aagaggccaa gcagcugcuc aaagugcaaa aggcuuauca ucagcuggau      720

cagucuuuca ucgacacaua cauugaucug cucgaaaccc ggagaacaua cuaugaggga      780

cccggcgaag gguccccuuu uggauggaag gacaucaaag agugguacga aaugcugaug      840

ggccacugca ccuauuuccc cgaggagcuu cgcagcguga aguacgccua uaaugcugau      900

cuguacaacg cccucaauga ccugaacaau cuugucauua cgcgggacga aaacgagaaa      960

cuggaauauu acgagaaguu ucagaucauc gaaaacguuu ucaaacaaaa gaagaaaccu     1020

acccucaagc agauugcgaa agagauccug gugaaugagg aagauauuaa gggcuacaga     1080

gugacaucua ccgggaaacc cgaguuuaca aaccugaagg ucuaucacga caucaaggau     1140

auuaccgccc gcaaagaaau caucgagaau gcugagcucc uggaccagau ugccaagauc     1200

cuuacgauuu accaguccag cgaagauauc caagaggaac ugacaaaccu caauucugag     1260

cugacccagg aagagauuga gcagaucucc aaccuuaaag gauauacagg cacccauaau     1320

cugagccuca aggcuaucaa ccugauucug gacgaacucu ggcacaccaa ugauaaccaa     1380

aucgccauuu ucaaucggcu gaaacuugug ccuaagaagg uugaccuguc ccagcagaaa     1440

gagaucccga caacgcucgu ggaugacuuu auucugucuc ccgucgugaa gcgcagcuuc     1500

auccagucca uuaaaguuau caacgccauc auuaagaaau acgggcugcc uaaugauauc     1560

auuaucgaac uugcucgcga gaagaacagc aaagacgcgc aaaagaugau uaaugaaaug     1620

cagaagcgga acagacagac caacgagcgc aucgaggaaa ucauucggac aaccggaaaa     1680

gagaaugcca aguaucucau cgagaaaauu aagcugcaug acaugcagga aggcaaaugu     1740

cuguacucuc ucgaagcuau cccccuggag gaucuucuga acaauccuuu uaacuaugag     1800

guggaccaca uuauccccag auccgucagc uucgauaauu cuuucaacaa uaaggugcuc     1860

gucaagcaag aagagaacag caaaaagggg aaucggacac cguuucagua ccuguccucu     1920

agcgacucca aaaucagcua cgaaaccuuc aagaaacaca uucugaaccu ugccaaggga     1980

aagggccgca ucucuaaaac gaagaaagag uaucuccugg aggaaagaga uauuaaucgg     2040

uuuuccgugc agaaggacuu caucaacaga aaucugguug auacacgcua cgccacccgg     2100

ggccucauga accugcuuag aagcuauuuu cgcgugaaua accuggacgu caaagugaag     2160

ucuauuaacg ggggauucac cuccuuucuc agacggaagu ggaaauucaa gaaagagcgc     2220

aauaagggcu acaaacauca cgcugaagau gcccugauca ucgcgaacgc ugacuuuauu     2280

uucaaggagu ggaaaaagcu ggauaaggcc aaaaagguua uggaaaauca gauguuugag     2340

gagaaacaag ccgaaagcau gccugagauc gaaacagagc aggaauauaa ggagauuuuc     2400

aucacacccc aucagauuaa acacaucaag gacuuuaagg auuacaaaua uucccauaga     2460

guggacaaga aaccuaaccg ggagcuuauu aaugacaccc ucuacucuac gcgcaaggau     2520

gacaaaggga acacccugau cgucaauaac cugaauggac ucuacgauaa ggacaacgau     2580

aagcugaaga aacuuaucaa uaaaagcccc gaaaagcugc ucauguauca ccacgacccc     2640

cagacauacc aaaaacugaa gcugauuaug gagcaguaug gcgaugaaaa gaacccucuu     2700

uacaaauauu acgaggaaac cgggaauuau cucacaaagu acuccaaaaa ggacaacggc     2760

ccggugauca aaaagauuaa guacuaugga aauaaacuga acgcucaucu ggauaucacc     2820

gacgauuacc ccaacucuag aaauaaggug guuaaacuca gccugaagcc uuaucgguuc     2880

gacgucuacc uugauaacgg cguguauaaa uucgugacgg ucaagaaucu ggacgugauu     2940

aaaaaggaga acuacuauga gguuaauucc aagugcuacg aagaggccaa aaagcucaaa     3000

aagaucagca accaggcuga auuuaucgcc ucuuucuaca auaacgaccu gauuaaaauc     3060

aauggggagc uguaucgcgu gauuggaguc aacaaugauc uucugaacag aaucgaggug     3120

aauaugauug acaucaccua ccgggaauau cucgagaaca ugaaugauaa gagacccccu     3180

agaaucauua agacaaucgc guccaaaacc caaagcauua agaaauacuc uacagacauc     3240

cugggcaacc ucuaugaagu uaaguccaaa aagcaccccc agauuaucaa gaaagggaag     3300

cggccggccg cuaccaaaaa ggccggacag gcuaaaaaga agaaauaagg acuaguaaua     3360

aggaaacugu augaaugucu gugggcagga agugaagaga uccuucugua aagguuuuug     3420

gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua aaucugguag aaaaaugaga     3480

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaagaa uu                                                         3552


<210>  4685
<211>  4620
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4685
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc ccuguucaac aagugcauua ucauuagcau caaucucgac      180

uuuuccaaca aagagaagug uaugaccaag ccguacucua uuggacugga uaucggcaca      240

aauagcguug ggugggcggu gaucacggac aacuauaaag ugcccuccaa gaaaaugaag      300

guccuuggaa auaccucuaa aaaguacauu aagaaaaacc ugcucggcgu gcugcuuuuc      360

gauagcggga ucacagcuga aggacggcgc cugaagagaa ccgcccggcg cagauauaca      420

cggcgcagaa aucggauucu cuaccugcag gagaucuuuu ccaccgaaau ggccacacug      480

gacgaugcuu ucuuucaacg ccucgacgau ucuuuccugg uuccugacga uaaaagagac      540

agcaaguauc ccauuuuugg caaccuugug gaggaaaaag ucuaccauga ugaguucccu      600

accaucuauc aucugcggaa guaccucgcc gacuccacga aaaaggcuga ucugcgccuu      660

guguaccugg cccucgcgca caugauuaag uauagaggcc acuuucugau cgagggggag      720

uucaauagca aaaacaauga cauccagaag aacuuucagg acuuccugga uaccuacaau      780

gccauuuuug aaucugaccu cucccuggaa aacagcaaac agcuugagga gaucguuaag      840

gauaaaauuu cuaagcugga aaagaaagac cggauccuca agcuguuccc cggagagaaa      900

aauuccggca uuuucagcga auuucuuaag cugaucgugg ggaaccaagc ugauuuccgc      960

aaaugcuuua accucgacga gaaggccucu cugcauuucu ccaaggaaag cuaugaugag     1020

gaccuggaga cacuccuggg auacaucggc gaugacuauu ccgaugucuu ucuuaaagcu     1080

aagaaacugu acgacgccau ucuccugucu ggguuccuua ccgugacaga caaugaaacc     1140

gaggcgccuc ugagcuccgc caugaucaag agauauaacg aacacaaaga ggaucucgcu     1200

cugcugaagg aauacauucg gaauaucagc cucaagacgu acaacgaggu guuuaaagac     1260

gauacaaaga auggauaugc cggcuacauu gacggcaaaa ccaaccagga ggauuucuau     1320

gucuaccuga agaaucuucu ggcugaauuu gagggggccg acuauuuccu cgaaaagauc     1380

gauagagagg acuuucugcg caagcagcgg acauucgaua acggaucuau cccguaccag     1440

auucaucugc aagaaaugag agccauccuu gacaaacagg cuaaguuuua ucccuuccuc     1500

gcgaagaaua aagagcgcau ugagaagauc cugaccuucc ggauuccuua cuacguuggc     1560

ccccuggcca gagggaacuc cgauuuugcu uggagcaucc gcaaacggaa ugaaaagauu     1620

accccuugga acuucgagga cgugaucgau aaagaaucua gcgccgaggc cuuuaucaau     1680

agaaugacau ccuucgaccu cuaucugccc gaggaaaagg ugcuuccgaa gcacucucug     1740

cucuacgaga cguuuaacgu cuauaaugaa cugaccaaag ugcgcuucau ugcugagagc     1800

augcgggacu accaguuucu ggauuccaag cagaaaaagg acaucguuag acuuuauuuc     1860

aaagauaagc gcaaggugac agacaaagau auuaucgaau accuccacgc cauuuaugga     1920

uacgacggca ucgagcugaa ggggaucgag aaacaauuua acagcucucu guccaccuac     1980

caugaucucc ugaacauuau caaugacaag gaguuccuug augacagcuc uaacgaagcg     2040

auuaucgaag agauuaucca cacacugacc aucuuugaag aucgggagau gauuaaacag     2100

agacucucca aguucgagaa uaucuuugac aagagcgucc ugaaaaagcu gucccggcgc     2160

cacuauacgg gauggggcaa acuuucugcu aagcucauua acggcaucag agaugaaaag     2220

agcgggaaua caauucugga cuaccugauc gacgauggaa ucuccaaccg gaauuucaug     2280

cagcucauuc augacgaugc ccugucuuuc aagaaaaaga uccaaaaggc ccagauuauc     2340

ggcgacgagg auaaagggaa cauuaaggaa gugguuaaaa gccuuccugg aucccccgcu     2400

aucaagaaag gcauucugca gagcaucaag aucguggacg agcucgucaa ggugaugggc     2460

gggagaaaac cugaaucuau ugucguggag auggcccgcg agaaucagua uaccaaccaa     2520

ggaaagucca auagccagca gcggcugaaa agacuggaaa agucucuuaa agagcucggc     2580

uccaagaucc ugaaggaaaa cauucccgcu aaacugagca agaucgauaa uaacgcccuc     2640

cagaaugacc gccuguaccu uuauuaccug caaaacggga aagauaugua uaccggagac     2700

gaucucgaca uugacagacu gucuaacuac gauaucgacc acaucauucc ccaggcguuu     2760

cugaaggaua auagcaucga caacaaaguu cuugugucca gcgccucuaa ucggggcaag     2820

uccgaugacu ucccuagccu ggaggucgug aagaaacgca agacauuuug guaccagcuc     2880

cugaaaucca agcucauuuc ucagagaaaa uucgauaacc ugaccaaggc ugagcgcggg     2940

ggacuucugc cggaagacaa ggccggcuuu auccaacggc agcucguuga gacaagacag     3000

auuacgaaac auguggcucg gcugcuggau gaaaaguuca auaacaaaaa ggacgagaau     3060

aaccgcgccg ucagaaccgu gaaaaucauc acacuuaaga gcacccuggu gucccaguuu     3120

cggaaagauu ucgaacucua uaagguucgc gagauuaaug acuuucacca ugcgcacgau     3180

gccuaccuga acgcugucau cgccucugcu cuccugaaga aauaucccaa acuugagccu     3240

gaguucgugu acggggacua ucccaaauac aauagcuuua gagaacggaa guccgccaca     3300

gaaaaagugu auuucuacuc uaacauuaug aauaucuuca aaaagagcau uucccuggcc     3360

gacggcagag ucaucgagcg cccucucauu gaagugaacg aggagaccgg agaaagcguu     3420

uggaauaaag agucugaucu ggcuacggug cggagagucc uguccuaccc ccaagugaac     3480

guugugaaga aagucgagga acagaaccac ggccuugacc gcgggaagcc gaaaggacug     3540

uuuaaugcga accucagcuc uaagccuaag cccaauucca acgaaaaucu ggugggcgcc     3600

aaagaguauc ucgauccuaa gaaauacggg ggauaugcug gcaucagcaa cucuuucgcc     3660

guucugguga aggggaccau cgagaaaggc gccaagaaga aaauuacaaa uguccuugaa     3720

uuucagggaa ucuccauucu ggaccggauc aacuacagaa aggauaaacu gaauuuccuc     3780

cuggagaagg gcuauaaaga cauugaacuu aucaucgagc ugcccaagua cagccucuuu     3840

gagcuguccg augggagccg ccggaugcuc gcuucuauuc ugagcaccaa caauaaaaga     3900

ggagaaaucc auaagggcaa ccaaauuuuc cuuucccaga aguuugugaa acugcuguau     3960

cacgccaagc gcaucucuaa uacaauuaac gagaaucauc ggaaauacgu ggaaaaccac     4020

aagaaagagu ucgaggaacu cuuuuacuau auccuggagu ucaaugaaaa cuacgucggg     4080

gcgaagaaga acggaaaacu ucugaauagc gcuuuucagu ccuggcagaa ccacucuauc     4140

gacgagcucu guagcuccuu cauuggcccu accgggucug aaagaaaggg ccuguuugag     4200

cucacgagcc ggggauccgc cgccgauuuc gaguuccugg gcguuaaaau cccccgcuau     4260

agagacuaca caccgagcuc ucuucugaag gaugcuaccc ugauucauca auccgugaca     4320

gggcucuaug aaacccggau cgaccuggcc aaacuuggag agggcaagcg cccugcggcu     4380

accaagaaag ccgggcaggc caagaaaaag aaauaaggac uaguaauaag gaaacuguau     4440

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     4500

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     4620


<210>  4686
<211>  3696
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4686
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc cugguacgcg agccugaugu ccgcucauca ccuccggguu      180

ggaauugacg ugggcaccca uucugugggg cuggccacac uucgcgucga ugaccacgga      240

acgccgaucg agcugcucag cgcccugucc cauauucacg auucuggcgu ggggaaggaa      300

gggaaaaagg accacgauac cagaaagaaa cuuagcggca ucgcucggcg cgccagacgg      360

cugcuccauc accgcagaac acagcugcaa cagcuggacg agguucuccg ggaucugggc      420

uuccccauuc cuacccccgg ggaauuucuu gaccugaacg agcagacaga uccuuaucgc      480

guguggagag uccgggcucg ccucguggaa gagaagcugc ccgaggaacu uagaggaccu      540

gccaucucca uggcgguucg gcauaucgcc cgccacagag gcuggcggaa uccguacucu      600

aaaguggaga gccugcucuc ccccgcugaa gagagcccuu ucaugaaggc ccugagagag      660

cgcauucugg cuaccacagg ggaaguccuc gacgauggaa ucacccccgg ccaggccaug      720

gcgcaagugg cccugacgca caacauuucu augcgggggc cugagggaau ccuuggcaaa      780

cugcaucagu ccgacaaugc uaacgaaauu agaaagaucu gcgcccgcca gggcgugagc      840

cccgaugucu guaagcagcu ccugcgggcu guuuuuaaag ccgacucucc gagagggucc      900

gccgugagcc gcguggcucc ugacccccuu ccuggacaag gcucuuuccg gagagcgccc      960

aagugcgauc ccgaguuuca gcgcuuccgg auuaucucca ucgucgccaa ucugagaauu     1020

agcgaaacca aaggggagaa ccgcccucuc acagcugacg agcggagaca cgugguuacc     1080

uuucugacag aagauuccca ggccgaccug accugggugg augucgccga gaagcucgga     1140

gugcaucggc gcgaccugag aggcacggcu guucacacag augacgggga acggucugcc     1200

gcgagaccgc ccaucgaugc uaccgaccgc auuaugcggc agacaaaaau cagcucccuu     1260

aagaccuggu gggaggaagc cgauagcgag caaagaggag ccaugauucg cuaucuguac     1320

gaggacccua ccgacucuga augugcugag aucaucgccg aacuccccga ggaagaucag     1380

gcuaaacugg acucccuuca ucugccugcc ggcagagcgg ccuauagccg ggagucucuc     1440

acagcucuga gcgaucacau gcuggccacg accgacgauc uccacgaggc ucgcaagaga     1500

cuguucggcg uggacgauuc cugggcgccc ccggccgaag ccauuaaugc uccugucggg     1560

aaccccucug uggaccggac acuuaagauc gucggacgcu accugagcgc cguggagucc     1620

auguggggca ccccugaagu uauucaugug gagcacguca gagauggguu uacaagcgag     1680

cggauggcug acgaacgcga uaaagccaau agacggcgcu auaacgacaa ucaggaggcc     1740

augaagaaaa uccaaagaga uuacggaaag gaaggcuaca uuucucgggg agacaucgug     1800

agacucgacg cucuggagcu gcagggcugc gcgugucuuu auugcggaac cacgaucggc     1860

uaccauacau gucagcucga ucacauuguu ccccaggccg ggccuggauc caacaaucgc     1920

cggggcaacc ugguggcugu cugcgaaaga uguaaucgca gcaaaucuaa cacccccuuc     1980

gccguguggg cccaaaagug cgggaucccg cacgugggag uuaaggaggc uauuggccgg     2040

gucagagggu ggcgcaaaca gaccccuaac acauccagcg aggaccugac ccggcucaag     2100

aaagaaguga ucgccagacu gcgccggaca caggaggauc ccgaaauuga cgagagaucc     2160

auggaaucug uggcguggau ggcuaaugag cuucaucacc ggaucgccgc cgcuuauccc     2220

gagacgaccg ucauggugua ccgcggcagc auuacagccg cggcuagaaa ggccgccgga     2280

aucgauuccc ggaucaaccu gauuggcgaa aaagggcgca aggacagaau cgaucggaga     2340

caucacgcug uugacgccuc uguggucgcu cucauggagg ccagcguggc gaagacccug     2400

gccgaacgcu ccagccugcg gggagagcag agacuuacag gcaaagaaca aaccuggaag     2460

caguauacgg ggucuaccgu uggagcucgc gagcauuuug agauguggcg gggccacaug     2520

cuccaccuga cagaacuguu caaugagaga cucgccgaag auaaagugua cgucacccag     2580

aacauucgcc ugcggcuuuc cgacgggaau gcucauacag ugaacccuag caagcugguu     2640

ucucacagac ucggcgaugg acugaccgug cagcaaaucg accgcgccug uacgccggcc     2700

cuguggugcg cucuuacaag agagaaagau uuugacgaga agaauggccu ccccgcgcgg     2760

gaagaccgcg ccauuagagu ccaugggcac gagaucaagu ccagcgauua uauccaggug     2820

uucucuaaac ggaagaaaac cgacagcgau cgcgacgaaa caccuuucgg agcuauugcc     2880

gugagaggcg gguuugucga gaucggaccc uccauucauc acgcccggau cuacagaguu     2940

gaaggcaaga aaccugugua cgcuaugcug cgcguguuca cccacgaucu gcucagccag     3000

cggcaugggg accuguuuuc ugccgucauu cccccucagu ccaucagcau gagaugcgcg     3060

gagcccaaac uucgcaaagc uaucaccaca ggcaacgcca cguaucuggg augggugguu     3120

gugggcgaug agcucgaaau uaaugucgac uccuucacca aguacgccau cgggcgguuu     3180

cuggaggauu ucccgaacac aaccagaugg cggauuugug gauaugacac aaauucuaag     3240

cugacccuua aaccuaucgu gcucgcugcc gaaggccugg agaaccccag cuccgcgguu     3300

aaugaaauug uggagcugaa gggguggcgc gucgcuauca acgugcucac gaaaguucac     3360

ccuacagugg ucagacggga ugcccuggga cgccccagau acucuagccg guccaaucuu     3420

ccgaccucuu ggaccaucga gaagcgcccu gccgcuacaa agaaggccgg ccaagcuaag     3480

aaaaagaaau aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     3540

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     3600

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3696


<210>  4687
<211>  4557
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4687
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc caccaagccg uacagcauug gacuggacau cggcacaaac      180

uccguugggu gggcggugac gaccgauaau uauaaagugc ccucuaagaa gaugaaaguc      240

cucggaaaca caagcaagaa auacauuaag aaaaaucugc uuggcgugcu ccuguucgac      300

uccgggauca ccgcugaggg acggcgccug aagagaacag cccggcgcag auauacccgg      360

cgcagaaacc ggauucuuua ccugcaggaa aucuuuucua cagagauggc cacccucgau      420

gacgcuuucu uucaacgccu ggaugacagc uuccugguuc cugaugacaa gagagauucc      480

aaauauccca ucuuuggcaa ucucguggaa gagaaggccu accaugacga guucccuacg      540

auuuaucauc ugcggaaaua ccuugcugau ucuaccaaga aagccgaccu gcgccucguc      600

uaccuggcgc uugcccacau gaucaaguau agaggccacu uucugauuga gggggaguuc      660

aacagcaaaa auaacgauau ccagaagaau uuucaggacu uccucgacac auacaacgcu      720

auuuuugaau ccgaucugag ccuggaaaau ucuaagcagc ucgaagagau cgugaaagac      780

aagauuucca aacuggagaa gaaagaucgg auccuuaagc uguuccccgg agaaaagaac      840

agcggcaucu uuucugaguu ccucaaacug auuguuggga aucaagccga cuuccgcaag      900

ugcuuuaacc uugaugaaaa ggcuucccug cauuucagca aagagucuua ugacgaagau      960

cucgagaccc ugcugggaua caucggcgac gauuauuccg acguguuucu caaagccaag     1020

aagcuguacg augcgauucu ucugagcggg uuccucacag ucaccgacaa ugagacggaa     1080

gccccucugu ccucugcuau gaucaaaaga uauaacgagc acaaggaaga ccuugcccug     1140

cucaaagagu acauucggaa caucagccug aagacauaca augaaguguu uaaagaugac     1200

accaagaacg gauaugcugg cuacaucgau ggcaagacaa aucaggagga cuucuaugug     1260

uaccugaaaa agcuccuggc cgaguuugaa ggggccgauu auuuccuuga gaaaauugac     1320

agagaagauu uucugcgcaa gcagcggacc uucgacaacg gauccauccc guaccagauu     1380

caccuccaag agaugagagc uauccuggau aaacaggcga aguuuuaucc cuuccuggcc     1440

aaaaauaagg aacgcauuga gaagauccuu accuuucgga ucccuuacua cgucggcccc     1500

cucgcuagag ggaacagcga cuucgccugg ucuauucgca aacggaauga gaagaucaca     1560

ccuuggaacu ucgaagaugu uauugacaaa gaguccagcg ccgaggcuuu uaucaauaga     1620

augacgucuu ucgaucugua ucugcccgaa gagaaggugc ucccgaaaca uagccugcuu     1680

uacgaaaccu uuaacgugua uaaugagcug acaaaggucc gcuucauugc cgaauccaug     1740

cgggacuacc aguuucucga cucuaagcag aaaaaggaua ucgugagacu guauuucaaa     1800

gacaagcgca aaguuaccga uaaggacauu aucgaguacc ugcacgcgau cuauggauac     1860

gauggcauug aacuuaaggg gaucgagaaa caauuuaaca gcucccucag cacauaccau     1920

gaccugcuga auauuaucaa cgauaaggag uuccucgacg auucuuccaa ugaagcuauu     1980

aucgaggaaa ucauucacac ccugacgauc uuugaggacc gggaaaugau uaaacagaga     2040

cuuagcaagu ucgagaacau cuuugauaaa ucugugcuga agaagcucuc ccggcgccac     2100

uauacaggau ggggcaaacu gagcgccaag cugauuaacg gcaucagaga cgagaaaucc     2160

gggaauacca uccuugauua ccucauugac gacggaaucu cuaaccggaa uuucaugcag     2220

cugauucaug augacgcccu gagcuuuaag aaaaagaucc aaaaagcuca gauuaucggc     2280

gaugaagaca aggggaacau caaggagguc gugaaauccc ucccuggauc ucccgccauu     2340

aagaaaggca uccugcagag cauuaagauc guugaugaac uugugaaagu caugggcggg     2400

agaaagccug aguccauugu ggucgaaaug gcucgcgaga aucaguauac caaccaagga     2460

aagagcaauu cucagcagcg gcugaaaaga cucgagaagu cccugaaaga acugggcagc     2520

aagauccuua aagagaacau ucccgccaag cucucuaaga ucgacaauaa cgcgcugcag     2580

aaugaucgcc uguaccucua uuaccugcaa aacgggaaag acauguauac aggagaugac     2640

cuugauaucg acagacuguc caauuacgac auugaucaca ucauucccca ggccuuccuc     2700

aaggacaaca gcaucgauaa uaaagugcug guuucuagcg cuuccaaccg gggcaagagc     2760

gacgaugugc cuucucugga agucgugaaa aagcgcaaga ccuucuggua ccagcuucug     2820

aaauccaagc ucauuagcca gagaaaauuu gacaaccuga caaaggccga gcgcggggga     2880

cucuccccgg aggauaaagc uggcuucauc caacggcagc ugguugaaac gagacagauc     2940

accaagcaug uggcccggcu ucuggacgag aaguuuaaua acaaaaagga ugaaaauaac     3000

cgcgcgguca gaacagugaa aauuaucacc cucaagucua cacuggugag ccaguuccgg     3060

aaagacuuug agcuguauaa gguucgcgaa auuaaugauu uccaccaugc ccacgacgcu     3120

uaccuuaacg ccgucguggc uuccgcccug cucaaaaagu aucccaagcu ggagccugag     3180

uuuguguacg gggauuaucc caaauacaau ucuuucagag aaagaaagag cgccaccgag     3240

aaagucuauu uuuacuccaa caucaugaau auuuucaaga aaucuaucag ccucgcugac     3300

ggccggguga ucgaacgccc ucugauugag guuaacgaag agacgggaga guccgugugg     3360

aauaaggaaa gcgaccuugc gaccguccgg agagugcugu cuuaccccca aguuaacgug     3420

gucaagaaag uggaggaaca gaaucacggc cucgaucgcg ggaagccgaa aggacuguuu     3480

aacgccaauc uguccagcaa gccuaaaccc aacucuaacg agaaucuugu uggcgcuaag     3540

gaauaucugg acccuaagaa auacggggga uaugccggca ucuccaacag cuucacagug     3600

cucgucaagg ggaccauuga gaaaggcgcc aagaaaaaga ucacaaaugu gcuggaguuc     3660

cagggaauuu cuauccucga ucggauuaac uacagaaagg acaaacugaa uuuucuucug     3720

gaaaagggcu auaaagauau cgagcugauc auugaacucc ccaaguacuc ccuguucgag     3780

cuuagcgacg ggucccgccg gaugcuggcu agcauccucu cuaccaacaa uaaaagagga     3840

gagauucaua agggcaacca aaucuuucug agccagaaau ucgugaagcu ccuguaucac     3900

gccaagcgca uuuccaauac gaucaacgaa aaucaucgga aauacgucga gaaccacaag     3960

aaagaauuug aggaacuuuu cuacuauauc cuggaguuua augagaacua cguuggggcg     4020

aagaaaaaug gaaagcugcu caacucugcu uuccagagcu ggcagaauca cuccauugau     4080

gaacuguguu cuagcuuuau cggcccuaca ggguccgaga gaaagggccu uuucgaacug     4140

accucucggg gaagcgccgc cgacuuugag uuccucggcg ugaaaauucc ccgcuauaga     4200

gauuacacac cguccagccu gcucaaggac gcuacccuga uccaucaauc ugugaccggg     4260

cuuuaugaaa cacggauuga ucuggccaaa cugggagagg gcaagcgccc ugcggcuacg     4320

aaaaaggccg ggcaggccaa gaaaaagaaa uaaggacuag uaauaaggaa acuguaugaa     4380

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4440

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4557


<210>  4688
<211>  3639
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4688
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc cgcggcuuuc aagccgaaca gcauuaauua cauccuggga      180

cucgacauug gcaucgccuc cguugggugg gccauggugg agauugauga agaggaaaac      240

cccauccggc ugaucgaccu uggagugcgc gucuuugaga gagcugaagu gccuaaaacc      300

ggcgauucuc uggccauggc ucggcgccuc gccagaagcg uucggcgccu gacaagacgg      360

cgcgcgcaua gacuucugcg gacgcgcaga cuccugaagc gggagggggu gcugcaggcc      420

gcuaauuucg acgagaacgg acucauuaag ucccugccca auaccccuug gcaacuucgc      480

gccgcugccc uggauagaaa acucacaccc cuggaauggu cugcgguccu ucugcaccuc      540

aucaagcauc ggggcuaucu gagccagcgc aaaaacgagg gcgaaaccgc cgacaaggag      600

cugggggcuc uccugaaagg aguggccggc aaugcucacg cccuucagac aggggauuuu      660

agaaccccug ccgagcuggc ucucaacaag uucgaaaagg aguccggaca uauucggaau      720

cagagaucug acuacagcca cacauuuucc cgcaaagauc ugcaagcgga acuuauccuc      780

cuguucgaga agcagaaaga auuuggcaac ccgcacguua gcgggggacu gaaggagggc      840

auugagaccc ugcucaugac gcagcggccc gcccugucug gcgacgcugu gcagaaaaug      900

cuugggcauu gcaccuucga accugccgag cccaaggccg cuaaaaauac auauaccgcc      960

gaaagauuua ucuggcugac aaagcucaac aaucugcgca uucuugagca aggauccgaa     1020

cggccucuga ccgauacgga gagagcgaca cucauggacg agcccuaccg caagagcaaa     1080

cugaccuaug cucaggcccg gaagcugcuc ggccuggaag auacagccuu cuuuaaaggg     1140

cuuagauacg gaaaggacaa cgcugaggcc ucuacccuga uggaaaugaa ggcuuaucac     1200

gccaucuccc gcgcgcucga gaaggaaggc cugaaggaca aaaagagccc gcugaaucuu     1260

ucuccugagc uccaggauga gaucgggacc gccuucuccc uguuuaaaac agacgaagau     1320

auuacgggac ggcugaagga cagaauccag cccgagauuc ucgaagcucu gcuuaaacau     1380

aucagcuucg auaaguucgu ccaaauuucc cugaaggccc uccggcgcau cgugccucug     1440

auggagcagg gcaaaagaua cgacgaggcu ugugccgaaa ucuacggcga ucacuauggg     1500

aagaaaaaca ccgaggaaaa gauuuaccug ccucccaucc ccgcggacga gauucggaac     1560

ccgguggucc uuagagcccu cucucaggcu cgcaaaguua ucaauggagu ggugcggaga     1620

uauggcagcc ccgcccgcau ucacaucgaa acagcuagag aggucgggaa guccuuuaag     1680

gaucggaaag agaucgaaaa gcgccaagag gaaaacagaa aagaccggga gaaggccgcc     1740

gcuaaauucc gcgaauacuu uccuaauuuc gugggagagc ccaagagcaa agauauucug     1800

aagcugagac ucuaugagca gcagcauggc aagugccugu acucugggaa agaaaucaac     1860

cuuggccggc ugaaugagaa gggauauguu gaaauugacc acgcgcuccc uuuuucccgc     1920

accugggacg auagcuucaa caauaaagug cugguccugg gcucugagaa ccagaauaag     1980

gggaaccaaa cacccuacga auacuuuaau ggaaaagaca acagcagaga guggcaggag     2040

uucaaggccc ggguggaaac cucccgcuuu ccgagaucua agaaacagcg gauccuucuc     2100

cagaaguucg augaggacgg cuuuaaagaa agaaaucuga acgauacgcg cuauguuaau     2160

cgguuccugu gucaauuugu ggcugacaga augcgccuca cagggaaggg caaaaagcgg     2220

gucuucgcca gcaacggaca gauuaccaac cugcuuagag gguucugggg ccugcgcaag     2280

gugcgggccg agaaugauag acaucacgcu cucgacgccg ucgugguugc gugcuccacc     2340

guggcuaugc agcagaaaau cacacgcuuu guccgguaca aggagaugaa cgccuucgau     2400

ggaaaaacca uugacaagga aacaggcgag gugcugcauc agaaaacgca cuuuccucag     2460

cccugggagu ucuuugccca ggaaguuaug aucagagugu ucgggaagcc ugauggaaag     2520

cccgaauuug aggaggcuga cacccuggaa aaacuucgga cacuccuggc cgagaagcug     2580

agcucucgcc cugaagcggu ccacgaguau gugacacccc ucuucguguc cagagcuccg     2640

aaucggaaaa ugagcggcca ggggcauaug gaaaccguua agucugccaa acgccuggau     2700

gagggagucu ccgugcuuag agugccucug acccaacuca agcugaaaga ccuggagaag     2760

auggucaacc gggaaagaga gcccaaacuu uacgaagccc ugaaagcucg ccucgaggcc     2820

cacaaggacg aucccgcuaa agccuuugcg gaaccuuucu auaaguacga caaagccggc     2880

aaucggacgc aacaggugaa ggcuguuaga guggagcaag uccagaagac cggggugugg     2940

guucgcaacc auaauggcau cgccgauaac gcuacaaugg ugcgggucga cguguuugag     3000

aaaggagaua aguauuaccu gguuccgauu uacagcuggc aaguggccaa aggcauccuc     3060

cccgacagag ccgucgugca ggggaaggau gaagaggacu ggcagcugau ugaugacucc     3120

uucaauuuua aauucucucu ucacccuaac gaucuggugg aagucaucac caagaaggcu     3180

cgcauguucg gauauuuugc gagcugucac cggggcacag ggaauauuaa caucagaauc     3240

caugaccucg aucacaaaau uggaaagaau ggcauccugg aggggauugg cguuaaaacc     3300

gcccuguccu uccagaagua ccagaucgac gagcuuggaa aagaaauucg ccccugcaga     3360

cugaagaagc ggccucccgu gcgcaaaaga ccugcugcca cgaagaaagc cggccaggcu     3420

aagaaaaaga aguaaggacu aguaauaagg aaacuguaug aaugucugug ggcaggaagu     3480

gaagagaucc uucuguaaag guuuuuggaa uuaugucugc ugaauaauaa acuuuuuuga     3540

aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            3639


<210>  4689
<211>  4395
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4689
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc caagaaaccg uacaccauug gacuggacau cggcacaaac      180

agcguugggu gggcggugcu cacggaucag uaugaccugg ugaagcggaa gaugaaaauu      240

gcuggagauu ccgagaagaa acaaaucaag aaaaauuucu ggggcguccg ccuuuuugac      300

gaagggcaga ccgccgccga uagacggaug gcucgcacag ccagacggcg cauugagaga      360

cggcgcaaca gaaucucuua ccugcaggga aucuucgcug aagagaugag caagaccgac      420

gccaauuucu ucugccggcu cuccgauucu uuuuaugugg acaacgaaaa gcgcaauagc      480

agacaucccu ucuuugcgac aauugaggag gaaguugagu accacaaaaa cuauccuacc      540

aucuaccauc ugcgggagga acuugucaau uccucugaga aggccgaucu gcgccucguc      600

uaucuggcuc uggcccacau uaucaaauac agaggcaacu uccucauuga aggcgcucug      660

gacacacaga auaccagcgu ggaugggauc uacaagcaau uuauucagac guauaaccag      720

guuuucgccu ccggaaucga ggacggcagc cuuaaaaagc uggaagauaa uaaagacgug      780

gcgaagaucc ucgucgagaa ggugacccgg aaagaaaagc uggagcgcau ucuuaaacug      840

uaccccgggg agaagucugc cggaauguuu gcucaguuca ucucccucau ugugggcagc      900

aaagggaacu uucaaaagcc uuucgaccug aucgaaaagu cugauauuga gugugccaaa      960

gacuccuaug aagaggaucu ggaaagccuc cuggcucuua ucggagacga guacgccgag     1020

cuguucgucg ccgcuaagaa ugcguauucu gccguugugc ucuccagcau cauuacagug     1080

gcugaaaccg agacaaacgc caaacugucc gccucuauga ucgaaagauu ugauacccau     1140

gaggaagacc uuggcgagcu gaaggcuuuc auuaaacucc aucugcccaa gcacuacgag     1200

gaaaucuuua gcaauacgga gaagcacggc uaugccgggu acauugaugg aaaaacaaag     1260

caggcggacu ucuacaaaua uaugaagaug acccuggaaa acaucgaggg cgcugauuac     1320

uuuaucgcca aaauugagaa ggaaaacuuc cuccggaagc agagaacauu ugacaauggg     1380

gccaucccuc accagcugca ucuugaggaa cuggaggcua uucuccacca acaggccaaa     1440

uauuacccgu uccugaagga aaacuaugau aaaaucaagu cccuugucac cuuucgcauu     1500

cccuacuucg ugggaccucu ggcuaauggc cagagcgagu uugccuggcu cacccggaaa     1560

gcggacgggg agaucagacc cuggaacauu gaagagaagg uugacuucgg aaaaucugcc     1620

guggauuuua ucgaaaagau gacaaauaag gacacguauc ugccuaaaga gaacguccug     1680

cccaagcacu cccucugcua ccaaaaauac cugguguaua augaacuuac caagguucgc     1740

uacaucaacg aucagggcaa aacaagcuau uucucuggcc aggagaagga gcagauuuuc     1800

aaugaccugu uuaagcaaaa acggaaggug aaaaagaaag aucucgaacu guuccugaga     1860

aacaugagcc augucgaguc cccgaccauc gaagggcuug aggacucuuu uaauagcucc     1920

uacagcacau aucacgaucu ccugaaggug ggaauuaagc aggaaauccu ggacaacccu     1980

gucaauaccg agaugcucga gaacauugug aaaauccuga cgguuuucga agauaagcgc     2040

augaucaaag agcagcuuca gcaauuuucu gacgugcugg auggcgucgu gcucaagaaa     2100

cuggaacgga gacauuacac agggugggga cgccuguccg cuaagcuccu caugggcauu     2160

cgggacaagc agagccaccu gaccauccug gauuaucuca ugaaugacga cgggcugaac     2220

agaaaccuua ugcagcugau uaaugauucu aaccucuccu ucaaaagcau cauugagaag     2280

gagcagguua ccacagccga caaagauauc caauccaucg uggcugaccu ggccggcucu     2340

cccgcgauua agaaaggaau ccugcagagc cuuaagauug ucgaugaacu cguguccgug     2400

augggcuacc cuccccagac caucguuguc gagauggccc gggaaaauca gacaacgggg     2460

aaaggaaaga acaauucucg ccccagauac aagagccugg agaaagcuau uaaggaauuu     2520

ggcucccaaa uccugaaaga gcauccuacc gacaaccagg agcuccggaa uaacagacug     2580

uaucuuuacu aucugcagaa ugggaaggau auguacacag gacaagaccu cgauauccac     2640

aaccugagca auuaugacau ugaucacauc gugccgcagu cuuucauuac cgacaacucc     2700

aucgauaauc uggugcuuac aagcucugcc ggcaaccgcg aaaaagggga cgacgugccc     2760

ccucucgaga uugugcggaa gagaaaggug uuuugggaaa aacuguacca gggcaaucug     2820

auguccaagc gcaaauucga uuaucucacc aaggcugaga gaggaggccu gacggaagcc     2880

gacaaagccc gguuuaucca ucgccagcuu guugagacca gacaaauuac aaagaacguc     2940

gcuaacaucc ugcaccagcg guucaauuac gagaaggaug accaugggaa caccaugaaa     3000

caggugcgca ucguuacacu caagagcgcg cuggugucuc aguuuagaaa acaauuccag     3060

cuguacaagg uccgggaugu gaaugacuau caccacgccc augaugcuua ccuuaacgga     3120

guuguggcca auacccugcu caaagucuau ccccagcugg aaccugaguu cguguacggc     3180

gacuaucacc aguuugauug guucaaggcc aacaaggcua cggccaaaaa gcaauuuuac     3240

acaaauauua ugcuuuucuu ugcgcagaaa gaccgcauca uugaugaaaa cggggagauc     3300

cugugggaca agaaauaucu ugacaccgug aagaagguca ugagcuacag acagaugaau     3360

auuguuaaaa agacagaaau ccaaaaagga gaguucucca aggcuaccau caaacccaag     3420

ggcaacagcu cuaaacugau uccgcggaag accaauuggg auccuaugaa guacgggggc     3480

cucgacuccc ccaacauggc cuaugccgug gugaucgagu acgcuaaagg aaagaauaaa     3540

cuggucuuug aaaagaaaau uauccgcgug acaauuaugg agagaaaggc cuucgaaaag     3600

gaugagaaag cguuucugga ggaacagggc uaucggcagc cuaagguucu ugcuaaacug     3660

cccaaguaca cgcucuauga gugugaagag gggagacgcc ggaugcuggc cagcgccaac     3720

gaagcucaga aaggaaauca acaggugcuc ccuaaccauc uggucacccu ucugcaccau     3780

gccgcuaacu gcgagguguc cgacggcaag ucucucgauu acaucgagag caauagagaa     3840

auguucgccg agcugcuggc gcacguuucc gaauuugcca agcgcuauac acuugcugag     3900

gccaaccuga auaaaaucaa ccagcucuuu gaacagaaua aggaggggga cauuaaagcu     3960

aucgcccaau cuuucgugga ucugauggcc uuuaacgcua ugggagcgcc cgccagcuuc     4020

aaguuuuucg agaccacaau ugaacggaaa agauacaaua accucaagga gcugcuuaau     4080

uccaccauca uuuaccaguc uaucacgggc cuguaugaaa gccgcaagcg gcuggacgau     4140

aaaagaccgg cugccacaaa gaaagccggg caggcuaaga aaaagaaaua aggacuagua     4200

auaaggaaac uguaugaaug ucugugggca ggaagugaag agauccuucu guaaagguuu     4260

uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua auaaaucugg uagaaaaaug     4320

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa gaauu                                                      4395


<210>  4690
<211>  4428
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4690
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc caagaaaccg uacagcauug gacuggacau cggcaccaac      180

uccguugggu gggcgguggu gacagaugac uauaaggucc ccgcuaagaa aaugaaggug      240

cucggaaaua cggauaaauc ucauauugag aagaaccugc uuggcgcccu ccuguucgac      300

agcgggaaua ccgccgaaga ucggcgccug aaaagaacag cucggcgcag auacacccgg      360

cgcagaaacc ggauccuuua ucugcaggag auuuucuccg aagagauggg aaagguugac      420

gauucuuucu uucaccgccu cgaagacagc uuccugguga cagaggauaa gagaggcgag      480

cggcauccua ucuuuggcaa ucuggaagag gaagucaaau accacgagaa cuuccccacc      540

aucuaucauc uccgccaaua ccuggccgac aauccugaga agguggaucu uagacugguu      600

uaucucgcuc uggcccacau uaucaaauuu cgggggcacu uccuuauuga aggaaaguuu      660

gacacacgca acaaugaugu gcagagacug uuccaggagu uucucgcggu cuacgacaac      720

accuucgaaa auuccucucu gcaggagcaa aacguccagg uggaagagau ccugacggac      780

aaaauuagca aguccgccaa aaaggaucgg gugcucaagc uguuucccaa ugagaaaagc      840

aacggcagau ucgcugaauu ucuuaagcug aucguuggga aucaggccga cuuuaaaaag      900

cauuucgagc ucgaagagaa agcuccucug caguuuucua aggauaccua cgaagaggag      960

cuugaagugc ugcucgccca aauuggagac aacuaugcgg agcuguuccu guccgccaag     1020

aaacucuacg auagcauccu gcuuucuggc auccugacag ugaccgacgu cgggacaaag     1080

gcuccgcucu ccgccagcau gauucagcgc uauaacgaac accagaugga ucuggcucag     1140

cuuaaacaau uuauccggca gaagcugucu gacaaauaca augagguguu cuccgauguu     1200

agcaaggacg gauaugccgg cuacauugau ggcaagacca accaggaagc cuuuuauaaa     1260

uaccucaagg ggcugcugaa uaaaaucgag ggauccggcu acuuccucga caagauugag     1320

agagaagacu uucugcgcaa acaacggacg uucgauaacg ggucuauccc ccaucagauc     1380

caccuucagg agaugagagc uauuauccgc cggcaggcgg aauuuuaucc uuuccuggcc     1440

gacaaucaag auagaauuga gaagcuccug acauuucgca uccccuacua ugugggaccu     1500

cuggcucggg gcaagagcga cuucgccugg cuuuccagaa aaagcgccga uaagauuacc     1560

cccuggaacu ucgacgagau cgucgauaaa gaaucuuccg cugaggccuu uaucaaucgc     1620

augacaaacu acgaccucua ucugccgaau cagaaggugc ugccuaaaca cagccuccug     1680

uacgaaaagu ucaccguuua uaacgagcuu accaaaguga aguacaagac agaacagggg     1740

aaaacggcgu uuuucgaugc uaauaugaag caggagauuu uugacggagu cuucaaagug     1800

uaccggaagg ucaccaaaga uaagcugaug gacuuucucg agaaggaguu cgaugaauuu     1860

agaaucgugg accugacagg ccuggacaaa gaaaacaagg uuuucaaugc cucuuauggc     1920

accuaccaug aucuuugcaa aauucucgac aaggauuuuc uggacaacag caaaaaugag     1980

aagauccugg aagauauugu gcucacacug acccuuuucg aggaccggga gaugauccgc     2040

aagagacugg aaaacuauuc cgaucuccug acgaaagagc aagucaagaa acuggaacgg     2100

agacacuaca cagggugggg acgccuuucu gccgagcuca uucauggcau ccggaacaag     2160

gaaagcagaa aaaccauccu ggacuaucug auugaugacg gaaauuccaa ccgcaauuuu     2220

augcagcuca ucaacgauga cgcucugagc uucaaggagg agauugccaa ggcucaggug     2280

aucggggaaa ccgauaaucu uaaccagguu gugucugaca uugccggcuc ccccgcgauc     2340

aaaaagggga uccugcaaag ccucaaaauu gucgacgagc uggugaagau caugggccac     2400

cagccugaaa auauuguggu ugagauggcc agagagaacc aguucacaaa ucagggacgg     2460

cgcaacucuc aacagagacu gaaaggccuu accgauucca ucaaggaauu ugggagccag     2520

auucucaaag agcaucccgu cgaaaauucc caacugcaga acgaccggcu guuccucuac     2580

uaucugcaga auggacgcga uauguacaca ggcgaggaac uugacaucga uuaccugucu     2640

caguaugaca ucgaucacau uaucccccag gcuuuuauua aggacaacag caucgauaau     2700

agagugcuca cguccucuaa ggagaaccgg gggaaaagcg acgaugugcc uuccaaggac     2760

gucgugcgca aaaugaaaag cuacuggucu aaacugcugu ccgccaaacu uauuacccaa     2820

agaaaguucg acaaccugac aaaagcugag cggggaggcc ucaccgauga cgauaaggcc     2880

ggguuuauca aacgccagcu gguugaaaca agacagauca ccaagcacgu ggcgcggauu     2940

cucgacgaga gauucaauac ggaaaccgau gagaacaaua aaaagauccg ccaagucaag     3000

auugugacac ugaaaagcaa ccuuguuucu aauuuucgga aggaguucga acuguauaaa     3060

gugagagaga ucaacgacua ccaucacgcc caugaugcuu aucucaaugc cgucauuggc     3120

aaggcucugc ugggagugua cccgcagcuu gaacccgagu uuguuuaugg cgacuacccu     3180

cacuuccacg ggcauaaaga aaacaaggcc accgccaaga aauuuuucua cuccaauauc     3240

augaacuucu uuaagaaaga ugacgugcgc acagauaaga auggagagau uaucuggaaa     3300

aaggacgaac acaucagcaa cauuaagaaa guccugucuu auccccaggu gaauaucgug     3360

aagaaagucg aggagcagac cggcggguuc agcaaggaau ccauucuccc uaaaggaaac     3420

agcgauaagc ugaucccccg gaaaacgaag aaguuuuacu gggacacaaa gaaguauggc     3480

ggguucgacu cuccgauugu ugcuuacucc auccucguga ucgcggauau ugagaaaggc     3540

aagagcaaaa agcugaagac cgugaaagcc cuugucggag ugacaaucau ggaaaagaug     3600

accuuugaga gagacccugu ugcuuuccug gagcgcaaag gcuaucggaa ugugcaagaa     3660

gagaacauua ucaagcuccc caaauacucc cuguuuaagc uggaaaacgg gagaaagcgc     3720

cuucuggccu cugcccggga gcuccagaaa ggaaaugaaa uuguccugcc uaaccaucuc     3780

ggcacccugc uuuaucacgc uaagaauauc cauaaagugg augagcccaa gcaccuggac     3840

uacguugaua aacacaagga cgaguucaag gaacugcucg auguggucag caacuuuucc     3900

aaaaaguaca cacuggccga ggggaaucuu gaaaagauca aggagcugua ugcgcagaac     3960

aauggagaag accucaaaga gcuggcuucu agcuucauua accuccugac guuuaccgcc     4020

aucggcgccc cugcuacauu caaguuuuuc gauaaaaaua uugacagaaa gcgguacacc     4080

uccacaaccg agauccuuaa cgccacgcug auucaucaau cuaucacagg gcuguaugaa     4140

acccgcauug aucucaauaa gcugggcgga gacaaaagac ccgcggcuac caagaaagcc     4200

ggccaggcca agaaaaagaa guaaggacua guaauaagga aacuguauga augucugugg     4260

gcaggaagug aagagauccu ucuguaaagg uuuuuggaau uaugucugcu gaauaauaaa     4320

cuuuuuugaa auaauaaauc ugguagaaaa augagaucua aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  4428


<210>  4691
<211>  3561
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4691
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc ccagacaaca aaccugagcu acauucucgg acuggaccuu      180

ggcaucgcgu ccguugggug ggcuguggug gagauuaaug aaaacgagga uccgaucgga      240

cugauugacg ucggcgugcg gaucuucgaa cgcgccgagg uucccaagac gggggaaucu      300

cucgcccuga gcagacggcu ugcucgcucc accagacggc ugauccgcag acgggcccau      360

cgccuccugc uggcuaaaag auuucucaag cgggagggga uucugucuac aaucgaucuu      420

gagaagggcc ugccuaauca agccugggaa cuccgcgugg cgggccugga gagacggcuu      480

agcgccauug aauggggagc uguccugcuc caccugauca aacaucgcgg auaucugucc      540

aagcggaaaa acgagucuca gaccaauaac aaggagcucg gcgcccugcu uagcggggug      600

gcucagaauc accagcugcu ccaauccgac gauuacagaa cacccgccga acuggcgcuu      660

aaaaaguucg ccaaggagga aggacauauu agaaaccagc gcggcgcuua uacccacaca      720

uuuaaucggc uggaccuccu ggccgagcug aaccuccugu ucgcucagca gcaccaauuu      780

gggaauccuc auugcaaaga acacauccag caguacauga ccgagcuucu gauguggcag      840

aagcccgccc ucagcggaga ggccauucug aaaaugcuug gcaaguguac gcaugaaaag      900

aacgaguuca aagcugcgaa acacaccuau ucugccgaaa gauuuguuug gcugacaaag      960

cucaauaacc ugcgcauccu ggaggauggc gcugaacggg cccucaauga ggaggaaaga     1020

caacugcuua ucaaccaccc uuacgagaag uccaaacuga ccuaugccca ggugcgcaag     1080

cuccuggggc ugagcgaaca ggcuauuuuc aaacaucuuc gguacucuaa ggagaacgcc     1140

gaauccgcga cauuuaugga gcucaaggcu uggcacgcca ucagaaaagc ccuggagaau     1200

caaggacuga aggacaccug gcaggaucuc gcuaaaaagc cggaccugcu ugaugaaauu     1260

ggcacggccu ucagccugua caaaacagac gaggauaucc agcaguaucu caccaacaag     1320

guccccaauu cugugauuaa cgcucugcug gugucccuua auuuugacaa auucaucgaa     1380

cucagccuga agucccugcg caagauccuc ccucugaugg agcaagggaa acgguacgac     1440

caggccugca gagagauuua uggacaucac uacggcgaag cgaaccagaa gacaucucag     1500

cuucugcccg ccaucccugc ucaagagauu cgcaaucccg ucguucuccg gacccugagc     1560

caggccagaa aagugaucaa cgcuauuauc cggcaguaug gguccccggc ccgcgugcau     1620

aucgaaaccg gaagagagcu gggcaagagc uuuaaagaac ggagagagau ucagaagcaa     1680

caggaggaua aucgcacaaa gcgggaaucu gcgguccaga aauucaagga gcuuuucucc     1740

gacuuuagcu cugaaccuaa aagcaaggau auccucaaau ucagacugua cgagcagcaa     1800

cacggcaagu gucuguauuc cgggaaagaa auuaacaucc accgccucaa ugagaaggga     1860

uacguggaga uugaccaugc ccugcccuuu ucuagaacgu gggaugacag cuucaacaau     1920

aagguucuug ugcuggcuuc cgaaaaccag aauaaaggca accagacccc uuacgagugg     1980

cuccaaggga agaucaacag cgaacggugg aaaaauuuug ucgcccuggu gcugggaucu     2040

cagugcuccg cugccaagaa acagcgccuu cucacacagg uuauugauga caacaaguuc     2100

aucgauagaa aucugaacga cacccgguau aucgcccgcu uucugagcaa uuacauucaa     2160

gagaaccucc ugcuuguggg caagaauaaa aagaacgucu ucacacccaa ugggcagauc     2220

accgcucugc ucagaucucg guggggccug auuaaagcgc gcgaaaacaa uaacagacac     2280

caugcccugg augcuaucgu ggucgccugu gccacgcccu ccaugcagca gaagauuaca     2340

cgguuuaucc gcuucaaaga ggugcacccu uauaagaucg agaauagaua cgaaaugguu     2400

gaccaagaga gcggagaaau uaucuccccg cacuuucccg agccuugggc uuauuuccgg     2460

caggagguga acauuagagu cuuugacaau caucccgaua ccgugcuuaa ggaaaugcug     2520

ccugaccgcc cccaggccaa ccaccaguuc guucaaccuc ucuucguguc ucgggcgccg     2580

accagaaaaa ugagcggcca ggggcauaug gagacaauca aguccgcuaa acgccuggcc     2640

gaaggaauuu cuguccuccg gaucccccug acccagcuua agccuaaccu gcucgagaau     2700

auggugaaca aagaaagaga gcccgcccug uacgcuggcc ugaaggcccg ccuugcggag     2760

uuuaaucaag auccugcuaa ggccuucgcc acacccuuuu auaaacaagg gggacaacag     2820

gugaaggcua uccggguuga acagguccag aaaagcggcg ugcuggugag agagaacaau     2880

ggggucgccg acaacgcuuc cauugugcgc acggauguuu ucaucaagaa uaacaaauuu     2940

uuccucgugc cgauuuacac cuggcagguc gccaagggca uccugccuaa uaaagcgauu     3000

guggcccaca agaacgaaga cgagugggaa gagauggaug agggagcuaa guuuaaauuc     3060

agccucuuuc ccaaugaccu gguugaacuu aagaccaaaa aggaguacuu cuuuggcuau     3120

uacaucgggc uggaucgggc cacaggaaac aucucucuca aagaacauga cggcgagauu     3180

uccaagggga aggauggagu guauagaguc ggcgugaaac uggcucugag cuucgaaaag     3240

uaccagguug acgagcuugg gaaaaaucgg cagaucugcc gcccccagca gagacagccu     3300

gugcggaagc gcccggccgc caccaaaaag gcuggccaag cgaagaaaaa gaaauaagga     3360

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     3420

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     3480

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaagaau u                                               3561


<210>  4692
<211>  3639
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4692
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc cgcggcuuuc aagccgaacc ccauuaauua cauccuggga      180

cucgacauug gcaucgccag cguugggugg gccauggugg agauugauga agacgagaac      240

ccuaucugcc ugaucgaucu uggagugcgg gucuuugaac gcgcugaggu gcccaaaacc      300

ggcgacuccc uggccauggc uagacggcuc gcccgcucug uuagacggcu gacacgcaga      360

cgggcgcauc gccuucugag agcccggcgc cuccugaaga gagaaggggu gcugcaggcu      420

gccgauuucg acgagaaugg acucauuaag agccugccua acacgcccug gcaacuucgg      480

gcugccgcgc uggaucgcaa acucaccccu cuggaguggu ccgccguccu ucugcaccuc      540

aucaagcaua gaggcuaucu gucucagcgg aaaaaugaag gcgagacagc ugacaaggaa      600

cugggggccc uccugaaagg aguggcugau aacgcccacg cccuucagac cggcgacuuu      660

agaacaccgg cugagcuggc gcucaauaag uucgagaagg aaagcgggca uauucgcaac      720

cagcggggag auuacuccca caccuuuucu agaaaagacc ugcaagccga gcuuauccuc      780

cuguucgaaa agcagaaaga guuuggcaau ccccacguua gcgggggacu gaaggaaggc      840

auugagacac ugcucaugac ccagcgcccu gcucuguccg gcgaugccgu gcagaaaaug      900

cuugggcauu guacguucga gcccgccgaa ccuaaggcug ccaaaaacac cuauacagcg      960

gagcgguuua ucuggcugac caagcucaau aaccugcgca uucuugaaca aggaagcgag     1020

agaccccuga cagacaccga acgggcuacg cucauggacg agccguacag aaagucuaaa     1080

cugacauaug cccaggcccg caagcugcuc ggccuggagg auaccgcuuu cuuuaaaggg     1140

cuucgguacg gaaaggacaa ugccgaggcu uccacacuga uggaaaugaa agccuaucac     1200

gcgaucagca gagcccucga aaaggagggc cugaaggaua aaaagucucc ucugaaccuu     1260

ucccccgaac uccaggacga gaucgggacc gcuuucagcc uguuuaaaac cgaugaggac     1320

auuacaggac gccugaagga ucggauccag ccugaaauuc ucgaggcccu gcuuaaacau     1380

aucucuuucg acaaguucgu ccaaauuucc cugaaggcuc ucagacggau cgugccccug     1440

auggaacagg gcaaacgcua cgaugaggcc ugcgcggaga ucuacggcga ccacuauggg     1500

aagaaaaaua cggaagagaa gauuuaccug cccccuaucc cggccgauga aauuagaaac     1560

cccguggucc uucgggcucu cagccaggcc agaaaaguua ucaacggagu ggugcgccgg     1620

uauggcuccc cugcucgcau ucacaucgag accgccagag aagucgggaa gucuuuuaag     1680

gacagaaaag agaucgagaa gcggcaagaa gagaaucgca aagacagaga aaaggccgcu     1740

gcgaaauucc gggaguacuu ucccaacuuc gugggagaac cuaagagcaa agauauucug     1800

aagcugcgcc ucuaugagca gcagcauggc aagugucugu acuccgggaa agagaucaau     1860

cuuggcagac ugaacgaaaa gggauauguu gagauugacc acgcccuccc cuuuagccgg     1920

acaugggaug acucuuucaa uaacaaagug cugguccugg gcuccgaaaa ucagaacaag     1980

gggaaucaaa ccccguacga guacuuuaac ggaaaagaua auagccgcga auggcaggag     2040

uucaaggcua gaguggagac aucucgguuu ccucgcagca agaaacagag aauccuucuc     2100

cagaaguucg acgaagaugg cuuuaaagag cggaaccuga augacaccag auauguuaac     2160

cgcuuccugu gccaauuugu ggccgaucgg augagacuca cggggaaggg caaaaagcgc     2220

gucuucgccu ccaauggaca gauuacaaac cugcuucggg gguucugggg ccugagaaag     2280

gugcgcgcug aaaacgaccg gcaucacgcc cucgaugcgg ucgugguugc uuguucuacc     2340

guggccaugc agcagaaaau caccagauuu guccgcuaca aggagaugaa ugccuucgac     2400

ggaaaaacaa uugauaagga gaccggcgaa gugcugcauc agaaaacaca cuuuccccag     2460

ccuugggagu ucuuugcuca ggaaguuaug auccgggugu ucgggaagcc cgacggaaag     2520

ccugaguuug aagaggccga cacgcccgag aaacugagaa cccuucucgc ggaaaagcug     2580

agcucccggc cggaggcugu ccacgaauau gugacaccuc uguucgugag ccgcgccccc     2640

aacagaaaaa ugucuggcca ggggcauaug gagaccguua aguccgccaa acggcucgau     2700

gaaggaguca gcgugcugcg cgugccccuu acacaacuga agcucaaaga ccuggagaag     2760

auggucaaua gagagcggga accuaagcug uacgaggcuc uuaaagccag acuggaagcu     2820

cacaaggaug acccggccaa agcguuugcc gagcccuucu auaaguacga uaaagcuggc     2880

aaccgcaccc aacaggugaa ggccguucgg guggaacaag uccagaagac gggggugugg     2940

guuagaaauc auaacggcau cgcugacaau gccaccaugg ugcgcgucga uguguuugag     3000

aaaggagaca aguauuaccu cguuccuauu uacucuuggc aaguggccaa aggcauccug     3060

cccgaucggg cugucgugca ggggaaggac gaggaagauu ggcagcucau ugacgauucc     3120

uucaacuuua aauucagccu gcacccuaau gaccuugugg aggucaucac aaagaaggcg     3180

agaauguucg gauauuuugc cuccugccac cgcggcaccg ggaacauuaa uauccggauc     3240

caugaccugg aucacaaaau uggaaagaac ggcauccucg aagggauugg cguuaaaaca     3300

gcucugucuu uccagaagua ccagaucgac gagcugggaa aagagauuag acccugucgc     3360

cuuaagaaga gaccucccgu gcggaaacgc ccggccgcca ccaagaaagc uggccaggcc     3420

aagaaaaaga aguaaggacu aguaauaagg aaacuguaug aaugucugug ggcaggaagu     3480

gaagagaucc uucuguaaag guuuuuggaa uuaugucugc ugaauaauaa acuuuuuuga     3540

aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            3639


<210>  4693
<211>  3645
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4693
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc caaguaccau guuggaauug acgugggcac cuucagcgug      180

gggcuggcgg cuaucgaggu cgaugacgcc ggaaugccga uuaaaacacu cucccuggug      240

ucucacaucc augauagcgg ccuugacccc gaugaaauua aguccgccgu uacgcggcug      300

gcuucuagcg ggaucgcccg cagaacccgg cgccucuaua gacggaagcg cagacggcug      360

cagcaacuug acaaauuuau ccagcgccag ggauggccug ugauugagcu ggaagauuac      420

uccgaccccc ucuauccuug gaaggucaga gcugagcugg ccgcgucuua caucgccgau      480

gaaaaagagc ggggcgagaa gcugagcgug gcucuccgcc acauugccag acaucggggc      540

uggcgcaacc ccuaugcuaa aguuuccagc cuguaccuuc cugacgggcc gucugaugcc      600

uucaaggcga ucagagaaga gauuaagcgg gccuccggac agcccgugcc ugaaacagcu      660

accgucggcc aaauggugac acugugcgag cucgggaccc ugaaacuuag aggagagggc      720

ggggugcuga gcgcccgccu ccagcagucu gacuaugcuc gggaaaucca ggagauuugu      780

agaaugcaag aaaucggaca ggagcuguac cgcaagauca uugaugucgu uuuugccgcc      840

gaauccccca aaggcagcgc uucuucccgg gugggcaagg acccucugca gcccgggaaa      900

aauagagcgc ucaaggccag cgacgcuuuc cagcgcuacc ggaucgccgc ccugauugga      960

aaccuuagag ugcgcgucga uggcgagaaa cggauccugu ccguggagga aaagaaucuc     1020

guuuuugacc accuggugaa ccuuacaccg aagaaagagc cugaaugggu caccauugcu     1080

gagauccugg ggaucgauag aggacaacuc auuggcacgg ccaccaugac agacgauggg     1140

gaacgcgcgg gagcucggcc cccuacccac gacacaaaua gaucuaucgu gaacagccgg     1200

auugcucccc ugguugauug guggaagacc gccuccgccc uggagcagca ugccauggug     1260

aaagcucuca gcaaugccga ggucgacgau uucgacucuc ccgaaggcgc gaaggugcag     1320

gccuuuuucg cugaucugga cgacgauguc cacgccaaac uugacucccu gcaucucccu     1380

gugggccgcg cugccuauag cgaggauacg cugguuagac uuacacggag aaugcugucu     1440

gacggggugg aucucuacac cgcgcgccug caagaauuug gaaucgagcc gagcuggaca     1500

cccccuacac cccggauugg cgaaccuguc gggaaccccg ccguggacag aguucugaag     1560

accguguccc gcuggcucga gucugcuacc aagacguggg gagccccgga gagagucauc     1620

aucgaacacg ugcgggaggg cuucgugacc gaaaaacgcg cuagagagau ggauggggac     1680

augcggcgca gagccgcccg gaaugcuaag cuguuucagg agaugcagga aaaacuuaac     1740

guucagggca agccuagccg cgcggaucug uggagauauc aauccgucca gcggcagaau     1800

ugccagugug ccuacugcgg aagccccauu acauucucua acuccgagau ggaccacauc     1860

gugccucgcg cuggccaagg gagcaccaau acaagagaaa accucguggc cgucugucau     1920

cggugcaauc agucuaaagg aaacaccccc uuugccauuu gggcuaagaa cacguccauc     1980

gagggcguga gcguuaagga agccguggag agaacacgcc acugggucac cgauaccggg     2040

augcggucca cagacuucaa aaaguucacc aaagcggugg uugagagauu ucagcgcgcu     2100

acaauggaug aagagauuga cgcccggucu auggaaagcg uggccuggau ggcuaaugag     2160

cugagauccc gcgucgccca gcauuucgcg ucucacggaa cgaccgugcg gguuuauaga     2220

ggcagccuga cagcugaagc ccgccgggcc uccgggauca gcggcaagcu gaaauuuuuc     2280

gacggagugg gcaagucuag acucgaucgg cgccaucacg cuauugacgc cgcugucauc     2340

gccuuuaccu ccgauuacgu ggcggagaca cuggccguga gaagcaaccu gaagcaaucu     2400

caggcucacc ggcaggaggc cccucagugg cgcgaauuua ccgggaaaga cgcugagcau     2460

agagccgccu ggcgggucug gugucaaaag auggaaaaac ucuccgcucu gcuuacggag     2520

gaucugagag acgaucgcgu uguggugaug agcaaugucc ggcucagacu gggaaacggc     2580

ucugcgcaca aggaaaccau cgggaaacug agcaagguga aacuuuccag ccagcucucu     2640

guuuccgaca uugauaaggc cagcuccgag gcucuguggu gcgcccugac acgcgagccc     2700

ggauuugacc cgaaggaagg ccucccugcc aaucccgagc ggcauaucag agugaacggg     2760

acccacgucu augcuggcga uaauauugga cuguuccccg ugucugccgg cagcaucgcg     2820

cuucgcgggg gauacgcuga acugggcucc ucuuuucacc augcccgggu uuacaaaauu     2880

acaagcggga agaaaccugc cuucgcuaug cucagagugu auaccaucga ccugcugccg     2940

uaccgcaacc aggaucuuuu cuccgucgag cucaagcccc aaacgauguc uaugagacag     3000

gccgagaaaa agcugcggga cgcgcuggcu acaggaaaug ccgaauaucu cggcuggcug     3060

gugguugacg augagcuugu ggucgacacc agcaagaucg ccacagauca ggugaaagcu     3120

guggaagccg agcuggggac cauucgcaga uggcgggucg acggcuucuu uuccccuagc     3180

aagcuccgcc ugagaccccu gcagaugucu aaagaaggaa ucaagaaaga guccgcuccu     3240

gagcuuagca agauuaucga ucggcccggc uggcucccug ccguuaacaa gcuguucucu     3300

gacgggaaug ugaccguggu cagacgcgau ucccugggac gggugagacu cgaaagcaca     3360

gcgcaccuuc ccguuacgug gaaagugcaa aagcgcccgg ccgcuaccaa aaaggccggc     3420

caggcuaaaa agaagaaaua aggacuagua auaaggaaac uguaugaaug ucugugggca     3480

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     3540

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3645


<210>  4694
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4694
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc cgcgcggauu cuggcuuucg acaucggaau uagcuccauc      180

ggcugggccu uuucugagaa cgaugaacuc aaggacugcg ggguucgcau uuucaccaaa      240

guggagaauc cgaagacagg agaaagccug gcccuuccca gacggcuggc ucgcuccgcc      300

agaaagcggc ucgcucgcag aaaagcccgg cugaaccauc uuaagcaccu gaucgcgaau      360

gaguuuaaac ucaacuacga agauuaucag ucuuucgacg agagccuggc caaggcuuac      420

aaaggcuccc ugaucucucc uuaugagcuc cgcuuuagag cccugaauga acuucugagc      480

aagcaagauu ucgcucgggu gauucuccau aucgccaagc gcagagggua cgacgauauu      540

aaaaacuccg acgauaagga gaaaggagcg auccugaagg ccauuaaaca gaaugaagag      600

aaacuugcua acuaucagag cgucggcgag uaccuguaua aagaauacuu ucagaaguuc      660

aaggaaaauu cuaaagaauu uacgaacgug cggaauaaga aagaguccua cgaacgcugu      720

aucgcccaga gcuuccucaa ggacgagcug aaacugauuu uuaagaagca aagagaguuc      780

ggcuuuucuu ucuccaaaaa guucgaagag gaaguucuca gcguggcuuu uuauaaacgg      840

gcccugaagg auuucucuca ccuugucggg aacugcuccu uuuucaccga cgagaaaaga      900

gcccccaaga auagcccucu ggcuuuuaug uucguggcgc ucacacgcau caucaaccug      960

cuuaauaacc ugaagaacac cgaaggcauu cucuacacaa aagaugaccu gaaugcccug     1020

cucaacgagg uucugaagaa uggaacccuu acauauaaac agaccaagaa acugcucggg     1080

cuguccgacg auuacgaguu uaagggagaa aagggcacgu auuucaucga guuuaaaaag     1140

uacaaagagu ucauuaaggc ucuuggggag cauaaccugu cucaggacga ucucaaugaa     1200

aucgccaaag acauuacccu gaucaaggau gagaucaaac ugaagaaggc ccucgcuaaa     1260

uaugaccuga accaaaauca gauugauagc cuuuccaagc uggaguuuaa agaccaccuc     1320

aacaucagcu ucaaggcccu gaaacuggug acaccccuua ugcucgaagg aaagaaguac     1380

gaugaggcgu guaaugaacu gaaccugaaa gucgcuauua augaggacaa gaaagauuuu     1440

cucccugccu ucaacgagac cuacuauaag gacgaaguga caaauccggu gguccugcgg     1500

gccaucaaag aguacagaaa gguucuuaac gcucugcuca agaaauaugg caaggugcac     1560

aaaauuaaua ucgaacuggc ccgcgaggug ggcaagaacc auucucagcg ggcuaaaauc     1620

gaaaaggagc agaacgagaa uuacaaggcc aaaaaggacg cggaacugga gugcgaaaaa     1680

cuugggcuca agauuaacuc caaaaauauc cugaagcuga gacucuucaa agagcaaaag     1740

gaauuuugug ccuauagcgg agagaagauu aaaaucucug aucugcagga cgagaagaug     1800

cuugaaauug aucacaucua ccccuauagc cgcuccuucg acgauucuua caugaacaaa     1860

guccuggugu uuaccaagca gaaucaagag aaacucaacc agacgccuuu cgaggcuuuu     1920

ggcaaugaca gcgccaagug gcagaagauu gaaguucugg cuaaaaaccu gcccacaaag     1980

aaacagaagc ggauccuuga uaaaaauuac aaggacaagg aacaaaagaa cuucaaggau     2040

agaaaucuca acgacacccg cuauaucgcc cggcuggugc ugaauuacac aaaagauuau     2100

cucgacuuuc ugccucuuuc cgaugacgag aacaccaagc ugaaugacac ccagaaaggg     2160

agcaaggucc auguggaggc gaagucugga augcucacau ccgcccugag acacacgugg     2220

ggcuucagcg cuaaagaucg caacaaccau cugcaccacg ccauugacgc uguuaucauu     2280

gccuacgcca auaacucuau cgugaaggcu uuuuccgauu ucaaaaagga acaggagagc     2340

aauuccgcgg aacuuuaugc caaaaagauu ucugagcucg acuacaagaa caaacggaag     2400

uuuuucgagc ccuuuagcgg guucagacag aaaguccugg auaagaucga cgaaaucuuc     2460

guguccaaac cggagcggaa gaaaccuucu ggagcucugc augaagagac cuuucgcaag     2520

gaagaggagu ucuaucaaag cuacggcggc aaggaagggg uccucaaagc ccuggagcuu     2580

ggaaagauua gaaaagugaa uggcaagauc guuaaaaacg gggauauguu ucggguggac     2640

auuuucaagc acaagaaaac aaauaaguuu uacgccgucc ccaucuauac cauggauuuc     2700

gcucugaaag ugcucccuaa caaggccguu gcgagaucca aaaagggaga aauuaaggac     2760

uggauccuga uggaugagaa uuacgaauuu ugcuucagcc uguauaaaga cucucuuauc     2820

cugauucaga caaaggauau gcaggagccc gaguuugugu acuauaacgc uuucaccucc     2880

agcacggucu cucucaucgu guccaaacau gacaauaagu uugaaacacu gagcaaaaac     2940

cagaagauuc ucuucaagaa ugccaacgag aaagaaguga ucgccaaguc uauuggcauc     3000

caaaaucuga aaguuuucga gaaguacauc gucagcgcuc uuggggaagu gaccaaagcc     3060

gaguuucgcc agcgggagga cuucaagaaa aagagacccg cggcuaccaa gaaagccggc     3120

caggccaaga aaaagaaaua aggacuagua auaaggaaac uguaugaaug ucugugggca     3180

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     3240

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3300

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3345


<210>  4695
<211>  3756
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4695
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc cagcgaccug guucucggac uggauauugg caucgggucc      180

gugggagugg gcauucuuaa caaggucacc ggggagauca uucauaaaaa uucucggauc      240

uucccggcgg cucaggccga aaacaaucug gugcgcagaa caaaccggca aggacgcaga      300

cucgcccggc gcaagaagca cagacggguu cgccugaaua gacuuuuuga ggaaagcggc      360

cugaucacgg acuucaccaa aauuuccauc aaccucaauc ccuaccagcu gcgggugaag      420

ggccugacag augagcucuc uaacgaggag cuguuuauug cucuuaaaaa uauggucaag      480

caucgcggga ucagcuaucu ggacgaugcc uccgacgaug gaaacucuag cgugggcgac      540

uacgcucaga uuguuaaaga aaauuccaag cagcucgaaa ccaagacacc ugggcaaauc      600

cagcuggaga gauaucagac cuacggacag cuucggggcg auuucacagu ggaaaaagac      660

gggaagaaac accgccugau uaacgucuuu cccaccagcg ccuauagauc ugaggcgcuc      720

cggauccugc aaacgcagca ggaguucaau ccucagauca ccgaugaauu uauuaacaga      780

uaccuggaga uccucacagg aaagcgcaaa uauuaccaug gccccggcaa ugaaaagucc      840

cggaccgacu acgggagaua ucgcacaagc ggagagaccc uggauaacau uuucggcauc      900

cuuauuggga aaugcacguu uuacccugac gaguuccggg ccgcuaaggc cucuuauaca      960

gcucaagaau uuaaccugcu caaugaccug aacaaucuua ccgugccgac agagaccaag     1020

aaacugucca aggaacagaa aaaccagauc aucaauuacg ugaagaacga gaaagccaug     1080

ggacccgcga aacucuucaa guauauugcc aaacugcuga gcugugaugu cgcugacauc     1140

aagggcuaca gaauugauaa aucugggaag gccgaaaucc acaccuuuga ggcuuaucgc     1200

aaaaugaaga cacucgaaac gcuggacauu gagcaaaugg aucgggagac ccuugacaag     1260

cuggccuacg uucucacacu gaauaccgaa agagagggaa uccaggaagc ccuugagcac     1320

gaguucgcug auggcuccuu cagccagaaa cagguggacg aacuggugca auuucgcaag     1380

gcgaacuccu cuaucuucgg caaagggugg cauaauuuua gcgucaagcu caugauggag     1440

cugauuccug aacuguacga gacauccgag gaacagauga ccauccucac gcggcuggga     1500

aaacagaaga caaccucuuc uuccaacaag accaaauaua uugaugagaa acuucugaca     1560

gaagagaucu acaaucccgu gguugccaag agcgugagac aggcuauuaa gaucgucaac     1620

gccgccauua aagaauaugg cgacuucgau aauaucguga ucgagauggc ucgcgaaacc     1680

aacgaggacg acgagaagaa agccauucaa aagauccaga aggcgaauaa agaugaaaag     1740

gacgcugcca ugcucaaagc cgcuaaccag uacaacggga aggccgagcu gccucacucu     1800

guuuuucaug gacacaaaca gcuggcuaca aagauucggc uuuggcacca acagggcgaa     1860

agaugccucu auacggggaa gaccaucagc auucaugauc ugaucaauaa cuccaaucag     1920

uucgaggugg accacauccu gccccucucu auuacauuug augacagccu ggccaacaaa     1980

guccuugugu acgcgaccgc caaucaggaa aagggccaac ggacaccgua ucaggcucug     2040

gauuccaugg acgaugccug gagcuuccgc gagcucaagg cuuuugucag agagucuaag     2100

acccugucca acaaaaagaa ggaauaccug cuuacggagg aagacaucag caaauucgau     2160

gugcggaaga aauuuauuga gagaaaucuc guugacaccc gcuacgccuc ucgggugguc     2220

cugaacgcgc ugcaggaaca uuucagagcc cacaagaucg auacaaaagu guccguugug     2280

cgcggacaau uuaccagcca gcucagacgg cauuggggca uugagaagac acgcgacacc     2340

uaucaccacc augcugucga cgcccugauc aucgcugccu ccucucagcu uaaucugugg     2400

aagaaacaga agaacacgcu cgugagcuac uccgaggauc aacugcugga cauugaaaca     2460

ggggagcuua ucucugauga cgaauauaaa gagagcgugu ucaaggcccc uuaccagcac     2520

uucguugaua cccucaaauc caaggaguuu gaagacagca uucuguucuc uuaucagguc     2580

gauuccaaau uuaauagaaa gaucagcgac gcuacaauuu acgcgacccg gcaggccaag     2640

gugggaaaag auaaggcuga cgagaccuau gugcugggca aaaucaagga uaucuacaca     2700

caagacgggu acgaugccuu caugaaaauu uauaagaagg acaaaucuaa guuucucaug     2760

uaccgccaug acccccagac guucgaaaaa gucaucgagc cuauucugga aaacuauccc     2820

aauaagcaga ucaacgagaa aggaaaggag gugcccugua auccuuuucu uaaguacaaa     2880

gaggaacacg gcuauauuag aaaguacucc aaaaagggga acggcccgga aaucaaaagc     2940

cugaaguauu acgauucuaa gcucggaaac cacauugaca ucacccccaa agauagcaau     3000

aacaagguug ugcugcaguc cgucagcccu uggcgggccg acguguacuu caauaaaaca     3060

accggcaagu augagauccu ggggcuuaaa uacgcugauc uccaauuuga aaagggaaca     3120

ggcaccuaua agauuucuca ggagaaauac aacgacauca agaaaaagga ggggguugau     3180

uccgacagcg aguucaaauu uacgcuguau aagaaugauc ugcuccuggu gaaagacaca     3240

gaaaccaagg aacagcaacu uuuccgcuuu cuguccagaa ccaugcccaa gcagaaacau     3300

uacgucgagc ucaagccuua ugauaaacag aaguucgaag gaggcgaggc ccugauuaaa     3360

gugcugggga acguugcgaa uagcggccag ugcaagaagg gacuuggcaa aagcaacauc     3420

uccauuuaca aggugcggac agacguccug gggaaucaac acaucaucaa aaacgaggga     3480

gacaagccca aacucgauuu caagcgcccg gcugccacca agaaagccgg ccaggcuaag     3540

aaaaagaaau aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     3600

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     3660

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3756


<210>  4696
<211>  4314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4696
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc cacccaguuc gagggauuua caaaccugua ccaaguuagc      180

aagacgcucc gguucgaacu gauuccgcag ggcaaaaccc uuaagcauau ccaggagcag      240

ggguuuauug aagaggacaa ggcgcgcaau gaucacuaua aagaacugaa gcccaucauu      300

gacagaaucu acaaaacaua ugcugaucaa ugccuccagc uggugcagcu ugacugggag      360

aaccuguccg ccgccaucga uucuuaccgg aaggagaaaa ccgaagagac acgcaaugcu      420

cucauugaag aacaggccac cuauagaaac gcuauccaug acuacuucau uggacggaca      480

gauaaucuga ccgacgccau caacaagcgc cacgcggaga uuuauaaggg ccuguuuaaa      540

gccgaacucu ucaaugggaa ggugcugaaa caacuuggaa cggucaccac aaccgagcau      600

gaaaacgcuc ugcucagauc cuuugauaag uucacaaccu acuuuagcgg cuucuacgag      660

aaucggaaaa acguguuuuc ugccgaagac aucagcacgg cuauuccuca ccgcaucguu      720

caggauaauu uccccaaguu uaaagagaac ugucacaucu ucacaagacu gauuaccgcc      780

gugccuuccc uucgggagca uuucgaaaau gucaagaagg cgaucggcau uuuugugucu      840

acaagcaucg aggaaguuuu cuccuuuccc uucuauaacc agcugcucac ccagacccaa      900

auugaccugu acaaucagcu gcucggggga aucagccgcg aggccggcac agagaaaauc      960

aaggggcuga acgaggugcu uaaccuggcu auucagaaaa augaugagac ggcccacauc     1020

auugcuucuc ucccucauag auuuaucccg cuguucaagc aaauucuuuc cgaccggaac     1080

acccugagcu uuauccucga agaguucaaa ucugacgaag aggucaucca guccuuuugc     1140

aaguauaaga cacugcugcg caaugaaaac gugcucgaga ccgccgaggc ccuguucaau     1200

gaacuuaaca gcauugaucu gacacacauc uuuauuucuc acaaaaagcu cgagaccauc     1260

uccagcgcuc ugugugacca uugggauacg cuuagaaaug cgcuguacga acggagaauu     1320

uccgagcuca caggaaaaau caccaagucu gccaaagaga aggugcagcg cagccugaag     1380

cacgaagaca uuaaccugca ggagaucauc uccgcugccg gcaaagaacu cagcgaggcc     1440

uucaagcaga aaaccucuga aauucugucc caugcucacg ccgcgcuuga ucaaccccug     1500

ccuacaaccc ucaagaaaca ggaggagaag gaaauccuua agagccagcu ggacucucuu     1560

cucgggcugu aucaucugcu cgauugguuu gcugucgacg agagcaauga aguugauccc     1620

gaguucuccg cccggcugac aggaauuaaa cuugaaaugg agccuucucu gagcuucuac     1680

aacaaggcca gaaauuaugc uacgaaaaag cccuacuccg uggagaaauu uaagcucaac     1740

uuccaaaugc cgacccuggc cagcggcugg gacgugaaua aagaaaagaa caauggcgcu     1800

auccuguuug ucaagaacgg gcuuuacuau cucggaauua ugccuaaaca gaagggccgc     1860

uacaaagccc ugucuuucga gcccacagaa aagaccuccg agggguuuga uaaaauguau     1920

uacgacuauu ucccugacgc ggccaagaug auccccaagu gcagcacaca gcugaaagcu     1980

gugaccgccc acuuucagac gcacaccaca cccauccucc ugucuaacaa uuucauugaa     2040

ccucuugaga ucaccaagga gauuuacgau cugaacaauc cggaaaaaga gcccaagaaa     2100

uuucaaacag cuuaugccaa gaagaccgga gaccagaaag gcuaccggga agcgcucugu     2160

aaguggaucg auuucacgag agacuuucug uccaaauaca caaagaccac aagcauugau     2220

cuguccucuc uucgcccuag cucccaguau aaagaccucg gggaguacua ugccgagcug     2280

aacccccugc ucuaccauau cucuuuccag cggaucgcug aaaaggagau uauggaugcc     2340

guugaaaccg gaaagcugua ucuuuuucaa aucuacaaua aagacuucgc uaagggccac     2400

cauggcaaac cuaaccugca cacccucuau uggacagggc uguucagccc cgagaaucug     2460

gccaagacgu ccauuaaacu uaacggacag gccgaacucu uuuacagacc uaagagccgc     2520

augaaacgga uggcucacag acugggcgag aagaugcuga auaagaaacu caaggaucag     2580

aaaaccccga uccccgacac acuguaccaa gagcuuuaug auuacgugaa ccaucgccug     2640

ucucacgacc ucuccgauga agcgcgggcc cugcugccua augucauuac caaggaggug     2700

agccaugaaa ucaucaaaga cagacgguuc acaucugaca aguuuuucuu ucacguuccc     2760

auuacccuua acuaucaggc ugccaauucc ccuagcaagu ucaaccagcg cgugaaugcc     2820

uaccugaaag agcaucccga aacgccgauc auugggaucg auagaggaga gcggaaccuc     2880

auuuauauca cagucauuga cucuaccggc aagauccugg agcagagaag ccucaacacc     2940

auccaacagu uugauuacca gaaaaagcug gacaaucgcg aaaaagagcg gguggcugcc     3000

agacaggcgu gguccgucgu ggggacaauu aaggaucuua agcaaggcua ucugagccag     3060

guuauccacg aaauugugga ccucaugauc cacuaccagg cugucguggu ucuggagaac     3120

cugaauuucg gauuuaaauc uaagcgcacc ggcauugccg aaaaagccgu guaccagcaa     3180

uucgagaaga ugcuuaucga uaaacugaac ugccucgucc ugaaggacua uccugcugag     3240

aagguugggg gagugcucaa ucccuaccag cugacagauc aguuuacguc cuucgccaaa     3300

augggcaccc aaagcggguu ccuguuuuau gugcccgcgc cuuacacauc caagaucgac     3360

ccgcugaccg gauucgucga ucccuuugug uggaaaacaa uuaagaacca ugaaucuaga     3420

aaacacuucc ucgagggcuu ugacuuccug cauuaugaug ugaagaccgg ggacuuuauc     3480

cugcacuuca agaugaaucg gaaccuuagc uuucagcgcg gccugccugg auucaugccc     3540

gcuugggaca uugucuuuga gaaaaaugaa acgcaguucg augccaaggg caccccuuuu     3600

aucgccggga aaagaauugu gcccguuauc gagaaccauc gguucacagg acgcuacaga     3660

gaccucuauc cugcuaauga acugaucgcc cuccuggagg aaaagggcau uguguuccgg     3720

gaugggucca acauccuucc caaacugcug gagaaugacg auucucacgc uauugacacc     3780

auggucgccc ucauccgcag cgugcugcag augagaaacu ccaaugcggc cacaggagaa     3840

gauuacauua acucuccggu ucgggaccuu aacggcgugu guuuugauag ccgcuuccaa     3900

aauccugagu ggcccaugga cgcugaugcc aacggggcuu accacaucgc ccugaagggc     3960

cagcuccugc ucaaucaucu gaaagagucc aaggaccuua agcugcagaa cggaauuagc     4020

aaucaggacu ggcuggccua uauccaagaa cucagaaaca aacggccugc ugcgaccaag     4080

aaagccggcc aggcuaagaa aaagaaguaa ggacuaguaa uaaggaaacu guaugaaugu     4140

cugugggcag gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau     4200

aauaaacuuu uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           4314


<210>  4697
<211>  4293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4697
gggagagcgg cucggccauu uugucccagu caguccggag gcugcggcug cagaaguacc       60

gccugcggag uaacugcaaa gaagcuuacc auggccccca agaaaaagag aaaagugggc      120

auccacgggg ucccugcugc cagcauuuac caggaguucg uuaacaagua uucccugucu      180

aaaacccucc gguuugaacu gaucccgcaa ggaaagacac uugagaauau uaaggcgcgc      240

ggccugaucc ucgacgauga aaagagagcu aaggacuaca aaaaggccaa acagauuauc      300

gauaaguauc aucaguucuu uaucgaggaa auucugagcu ccgugugcau cucugaggac      360

cuucugcaga acuacagcga uguguauuuc aagcucaaaa aguccgacga ugacaaucug      420

caaaaagauu uuaagucugc caaagacacg auuaagaaac agaucagcga guacauuaag      480

gauuccgaaa aguucaaaaa ccuguuuaau cagaaccuca ucgacgcuaa gaaagggcag      540

gagagcgauc ugauucuuug gcugaagcaa ucuaaagaca auggaaucga acucuucaag      600

gccaacuccg acaucaccga uauugacgag gcucuggaga ucauuaagag cuuuaaaggc      660

uggacaaccu auuucaaggg guuucacgaa aaucggaaaa acgucuacuc uuccaaugau      720

auccccacaa gcauuaucua ccgcaucgug gacgauaacc uuccuaaguu ccuggagaau      780

aaagccaagu augaaucucu caaggacaaa gcgcccgagg ccauuaacua cgaacagauc      840

aagaaagauc uggcugagga gcugaccuuu gacauugauu auaagacauc cgaaguuaau      900

cagagagugu ucagccucga cgaggucuuc gaaaucgcca acuuuaacaa uuaccugaac      960

caguccggaa uuaccaaauu caauacgauc aucggcggca aguuugugaa cggggagaau     1020

accaagcgga aaggaauuaa cgaauauauc aaucuuuacu cucaacagau uaacgauaag     1080

acacugaaaa aguauaaaau gagcguucuc uucaagcaga uccuguccga caccgagagc     1140

aaaucuuuug ugauugacaa acuugaggau gacuccgaug ucgugacaac caugcagagc     1200

uucuacgaac aaaucgcugc cuuuaagacg guggaggaaa aaucuauuaa ggagacacug     1260

agccuccugu ucgacgaucu gaaagcgcag aagcucgacc uguccaaaau cuacuuuaag     1320

aaugauaagu cucuuaccga ccugagccag caagucuucg augacuauuc cguuaucggc     1380

acagccgugc ucgaauacau uacccagcag aucgcuccua aaaaccugga uaaucccagc     1440

aagaaagagc aggagcuuau ugccaagaaa accgaaaagg cuaaguaucu gucucucgag     1500

acaaucaaac uggcccugga agaguuuaac aagcaucgcg acauugauaa acaauguaga     1560

uucgaggaaa uccucgccaa uuuugcugcg aucccuauga uuuucgacga gaucgcccag     1620

aacaaggaca aucuggcuca gauuuccauc aaauaccaga accaagggaa gaaggaucuu     1680

cugcaggcca gcgccgaaga cgaugugaaa gcuauuaagg accuccugga ucagacgaac     1740

aaucugcuuc acaaacucaa gaucuuccau aucucucagu ccgaggacaa agccaacauu     1800

cuggauaagg acgaacacuu uuaucugguc uucgaggagu gcuacuuuga acucgcgaau     1860

aucgugccgc uguauaacaa aauucggaau uacaucaccc aaaagcccua cagcgaugag     1920

aaguucaaac uuaacuuuga aaauuccaca cuggcuaacg gaugggacaa gaauaaagag     1980

ccugauaaca ccgccauucu cuucaucaag gacgauaaau auuaccuggg cguuaugaau     2040

aagaagaaca auaaaaucuu ugacgacaag gccauuaaag aaaacaaggg ggagggauau     2100

aaaaagaucg uguacaagcu gcuucccggc gcuaauaaaa ugcucccuaa ggucuucuuu     2160

ucugccaaaa gcauuaaguu cuauaacccc uccgaggaua uccugcgcau uagaaaccac     2220

ucuacacaua ccaaaaaugg cagcccgcag aagggguacg aaaaguuuga guucaacauc     2280

gaagacuguc ggaaauuuau ugauuucuau aagcagucca ucagcaaaca cccugagugg     2340

aaggacuucg gauuucgcuu cucugauacg caaagauaca auuccaucga cgaauuuuac     2400

cgggaggugg agaaccaggg cuauaaacug acauucgaaa auauuagcga gucuuacauc     2460

gauuccguug ugaaccaggg gaagcucuau cuguuucaga uuuacaauaa ggacuucagc     2520

gcuuauucua aaggacgccc caaccuucau acccuguacu ggaaggcccu cuuugaugaa     2580

agaaaucugc aagacgucgu guauaaacug aacggcgagg cggagcuuuu cuaccggaag     2640

cagagcaucc cuaaaaagau uacccacccc gccaaagaag cuaucgccaa uaagaacaag     2700

gauaauccca aaaaggaguc cgucuuugaa uacgaccuca ucaaagacaa gagauucaca     2760

gaggauaaau uuuucuucca cugcccuauu accaucaacu uuaagagcuc uggggcuaau     2820

aaguucaacg acgaaauuaa ccugcugcuc aaagagaagg ccaaugaugu gcauauccug     2880

uccauugacc gcggagagcg gcaccuugcg uauuacacac ugguugaugg caaaggcaac     2940

aucaucaagc aggacacguu uaauauuauc gggaacgaua gaaugaaaac caauuaucau     3000

gacaagcucg ccgcuauuga aaaggaucgc gacagcgccc ggaaagauug gaagaaaauc     3060

aacaauauua aggagaugaa agaaggauac cugucccagg ugguccacga gaucgcuaag     3120

cuggugauug aauauaacgc caucguugug uucgaggacc uuaauuuugg cuucaagaga     3180

gggcgcuuua aagucgagaa gcaaguguac cagaaacucg aaaagaugcu gaucgagaaa     3240

cugaacuauc ucguguucaa ggauaaugaa uuugacaaaa caggaggcgu ucugcgggcc     3300

uaccagcuua ccgcuccguu cgagacauuu aagaagaugg ggaaacagac cggcauuauc     3360

uacuaugucc ccgcgggauu cacgucuaag auuuguccug ugaccggcuu ugugaaccaa     3420

cuguacccca aauaugaaag cgucuccaag ucucaggagu ucuucagcaa auuugacaag     3480

aucugcuaca aucucgauaa gggguauuuc gaguuuuccu ucgacuacaa aaacuuugga     3540

gauaaggccg cuaaaggcaa guggacaauu gccucuuucg ggagcagacu gaucaauuuu     3600

cgcaacuccg acaaaaauca uaacugggau acccgggaag uguauccuac aaaggagcug     3660

gaaaaacuuc ugaaagacua cagcaucgag uacggacacg gcgaguguau uaaggccgcu     3720

aucugcgggg aaucugauaa aaaguucuuu gccaaacuca ccuccguucu gaacacgauu     3780

cuccagauga gaaauagcaa gacaggcacc gagcuggacu aucuuaucuc ucccguggcg     3840

gaugucaacg gaaauuucuu ugacucccgc caagcuccga agaacaugcc ucaggaugcc     3900

gacgccaaug gcgcuuacca cauugggcug aaaggacuca ugcugcuggg ccggaucaag     3960

aacaaucagg aagggaaaaa gcugaaccuu gugaucaaaa augaggaaua uuucgaguuu     4020

guucagaaca gaaauaacaa gcggcccgcc gcgacaaaga aggcuggaca agccaagaaa     4080

aagaaauaag gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag     4140

auccuucugu aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau     4200

aaaucuggua gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  4293


<210>  4698
<211>  4439
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4698
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccgac aagaaauaca gcauuggacu ggauaucggc      180

accaacuccg uugggugggc ggugauuaca gacgaguaua aggugccguc uaagaaauuc      240

aagguccucg gaaauacgga ucggcauagc aucaaaaaga accugauugg cgcucugcug      300

uuugacuccg gggaaaccgc cgaggccaca cgccucaaaa gaaccgcucg gcgcagauac      360

acacggcgca agaauagaau cugcuaucug caggaaaucu ucucuaacga gauggccaag      420

guggaugaca gcuuuuucca ccggcuugaa gaguccuuuc ugguugagga agauaaaaag      480

caugagcgcc accccauuuu cggaaauauc guggacgaag ucgcuuacca ugagaaauau      540

ccuaccauuu accaccucag aaagaaacug guggauucua cagacaaggc cgaucugcgg      600

cucaucuauc uggcgcuugc ccacaugauu aaauuucgcg gccauuuccu gaucgagggc      660

gaccucaacc ccgauaauag cgacguugac aagcuguuua uucaacuugu gcagaccuac      720

aaccagcugu ucgaagagaa uccuaucaac gcuuccgggg ucgaugccaa ggcuauccuc      780

agcgccagac ugucuaaauc ccggcgccug gaaaaucuca uugcgcagcu gcccggagag      840

aagaaaaacg gccuuuuugg gaaucugauc gcccucagcc ugggacuuac gccuaacuuc      900

aagucuaauu uugaccuggc ugaagaugcc aaacuccaac uguccaagga caccuacgau      960

gacgaucugg acaaccuccu ggcucagauu ggcgaucagu augccgaccu uuuccuggcc     1020

gcuaagaacc ucagcgaugc gauccugcuu ucugacauuc ugagagugaa uacagagauc     1080

accaaagccc cgcucuccgc uagcaugauc aagcgguacg acgagcacca ucaggaucug     1140

acacugcuca aagcccuggu gagacaacag cuucccgaaa aguauaaaga gauuuucuuu     1200

gaccagucca agaacgggua cgccggauau aucgauggcg gcgcuucuca ggaagaguuc     1260

uacaaguuua uuaaaccuau ccuggaaaag auggacggga ccgaggagcu ccuggucaaa     1320

cugaaucgcg aagaucuucu ccggaagcaa agaacguucg acaacggaag cauuccccac     1380

cagauccacc ugggcgagcu gcaugccauc cuccgccggc aggaagauuu uuauccuuuc     1440

cugaaagaca auagagagaa gauugaaaag auccuuacau uucgcauucc cuacuacguu     1500

gggccgcugg cgcggggaaa cuccagauuc gcuuggauga cccgcaaaag cgaggagaca     1560

aucaccccuu ggaauuuuga agagguggug gauaagggcg ccucugccca auccuucauu     1620

gaacggauga ccaacuuuga caaaaaucuc cccaacgaga agguccugcc uaaacacagc     1680

cugcuuuaug aguacuucac aguguauaau gaacucacga agguuaaaua cgugaccgag     1740

gggaugagaa agcccgcuuu ucugucugga gaacagaaga aagccaucgu cgaucuucuc     1800

uucaagacaa accgcaaagu gaccguuaag cagcugaaag aggacuauuu caagaagauu     1860

gaauguuuug auagcgugga gaucuccggc gucgaggacc gguucaaugc uucucuuggc     1920

acauaccaug accugcucaa aaucauuaag gauaaagacu uucuggauaa cgaagagaau     1980

gaagacaucc uggaggauau ugugcuuacc cucacgcugu ucgaagacag agagaugauc     2040

gaggaacggc ugaagacaua ugcccaccuc uuugaugaca aagucaugaa gcagcugaag     2100

cgcagacggu acaccgggug gggaagacuu agccgcaaac ugauuaacgg cauccgggau     2160

aagcaauccg ggaaaaccau ccucgacuuc cuuaagagcg auggauuugc gaacagaaau     2220

uucaugcagc ugauucauga cgauucucug acauuuaaag aggacaucca gaaggcccag     2280

guguccggcc aaggggacag ccuccacgaa cacauugcua accuggccgg cucucccgcu     2340

aucaagaaag gcauucugca gaccguuaag guggucgaug agcucgugaa aguuauggga     2400

cgccauaagc cugaaaauau cgugaucgag auggccagag agaaccagac aacgcagaaa     2460

gggcaaaaga acucccggga acgcaugaaa agaauugagg aaggaaucaa ggagcugggc     2520

agccagauuc uuaaggagca cccggucgaa aauacccagc ugcagaauga gaaacucuac     2580

cuguauuacc ugcaaaacgg gcgggacaug uauguggauc aggaacuuga caucaaucgc     2640

cuguccgauu acgacgugga ucauauuguu ccccagucuu uccucaagga cgauagcauc     2700

gacaacaaag uccugacaag auccgauaag aaucggggaa aaucugacaa cgugccuagc     2760

gaggaagugg ucaagaagau gaaaaauuau uggcgccaac uccugaacgc gaaacuuauc     2820

acccagagaa aauuugacaa ucugacaaag gccgagcggg gcgggcucuc cgagcuggau     2880

aaagcuggcu ucauuaagcg ccagcuggug gaaaccagac agaucacgaa gcacguugcc     2940

caaauucuug acagccggau gaacaccaaa uacgaugaga acgacaagcu gaucagagaa     3000

gugaaaguca uuacacucaa gucuaaacug guguccgauu uucgcaagga cuuccaguuc     3060

uauaagguuc gggagaucaa uaacuaccac caugcucacg augccuaccu caaugccgug     3120

gucggaaccg cucugauuaa aaaguauccc aaacuugaaa gcgaguuugu guacggcgac     3180

uauaagguuu acgaugugag aaaaaugauc gcgaagucug agcaggaaau cgggaaggcc     3240

acagcuaaau auuucuuuua cuccaacauu augaauuucu uuaagaccga gaucacgcug     3300

gccaacggag aaauucgcaa acggccucuc aucgagacaa auggcgaaac cggggagauu     3360

gucugggaca agggaagaga uuucgccaca gugcgcaaag ugcugagcau gccccagguc     3420

aacaucguua agaaaaccga ggugcaaacc ggcggguuuu cuaaggaaag cauccugccg     3480

aagagaaauu ccgacaaacu uauugcucgg aagaaagauu gggacccuaa gaaauauggc     3540

ggauucgaca gccccacagu ggccuacucu guccuggugg uugcgaaggu ggagaagggc     3600

aaauccaaga aacucaagag cgucaaagaa cugcucggga ucacgauuau ggagcgcucc     3660

ucuuuugaga agaacccuau cgauuuccug gaagcuaagg gauacaaaga ggugaagaaa     3720

gaccuuauua ucaagcugcc caaauauagc cuguuugaac ucgagaaugg ccggaagaga     3780

augcuggccu ccgccgggga acuucagaag ggaaacgagc uggcucuccc uucuaaauac     3840

guuaauuucc uguaucucgc cagccauuac gagaagcuga aaggcucccc cgaagauaac     3900

gagcagaagc agcuuuucgu ggaacaacac aaacauuauc uggacgagau cauugaacag     3960

aucucugagu uuagcaagcg ggucauucug gcggaugcua aucucgacaa agugcugucc     4020

gccuacaaca agcaccgcga uaagccgauc agagagcagg ccgaaaacau uauccaccuu     4080

uucacccuga caaaucucgg ggcuccugcc gcuuuuaaau auuucgacac cacaauugau     4140

cggaagcgcu acaccagcac gaaagagguu cuggacgcca cacucaucca ucaaucuauc     4200

accggccugu acgaaaccag aauugaucuu ucccagcugg gaggcgacaa gcggcccgcg     4260

gccacaaaga aggcugggca ggccaagaaa aagaaauaag gacuaguccc uguucccaga     4320

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      4439


<210>  4699
<211>  5222
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4699
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaac uucaagauuc ugccgaucgc gauugaccuc      180

ggaguuaaaa auacaggcgu guuuagcgcu uucuaccaga aggggacauc ccuggagcgg      240

cuugauaaca agaauggaaa aguguaugaa cugucuaagg acagcuacac gcuccugaug      300

aacaaucgca ccgccagacg gcaucaacgc agaggcaucg aucggaaaca gcuugucaag      360

cgccuguuua aacucauuug gacagagcag cugaaccugg aaugggacaa ggauacccag      420

caagccaucu ccuuccucuu uaauagacgg ggguucucuu uuaucacaga cggauauagc      480

cccgaguacc ugaacauugu gccugaacag guuaaggcua uccuuaugga uauuuucgac      540

gauuauaaug gcgaggacga ucuggacucc uaccucaaac uggccaccga gcaggaaucu      600

aagaucagcg agauuuauaa caaacuuaug cagaagaucc uggaauuuaa acucaugaag      660

cugugcacag auauuaaaga cgacaaggug uccaccaaga cgcugaaaga gaucaccagc      720

uacgaguucg aacuccuggc ugauuaccuu gccaauuauu cugagucccu gaagacacaa      780

aaauuuagcu acaccgacaa gcagggcaac cucaaagaac ugucuuauua ccaccaugau      840

aaguauaaua uccaggaguu ccuuaagcgc cacgcgacaa uuaacgacag aauccuggau      900

acccuccuga cggacgaucu ggacauuugg aauuuuaacu ucgaaaaauu cgauuuugac      960

aagaacgagg agaaacucca gaaucaagaa gauaaggacc auauccaggc ccaucugcac     1020

cacuucgucu uugcugugaa caaaauuaag uccgagaugg ccagcggggg acggcaccgc     1080

ucucaguacu uccaagaaau cacaaauguu cuugacgaga acaaucauca ggaaggcuau     1140

cugaagaacu uuugugagaa ucuccacaac aaaaaguacu ccaaucugag cgugaaaaac     1200

cuugucaauc ugaucgggaa ccucuccaau cuggagcuga agccccucag aaaauacuuc     1260

aacgauaaga uucacgcuaa ggccgaccau ugggaugaac agaaauuuac cgagacauau     1320

ugccacugga uccugggaga auggcgggug ggcgugaagg accaggauaa aaaggacggg     1380

gcgaaauacu cuuauaagga ucuuuguaau gagcugaaac aaaaggucac caaggccgga     1440

cucguugacu uccugcugga acuugauccu ugccgcacca uucccccuua ccucgacaac     1500

aacaauagaa aaccgcccaa gugucagagc cugauccuga acccuaaauu ucucgauaau     1560

caguauccca acuggcagca auaccugcag gagcuuaaga aacugcaguc cauucagaau     1620

uaucucgaca gcuucgagac agaucugaag gugcugaagu cuuccaaaga ccaaccuuac     1680

uuuguggaau acaagagcuc uaaccagcag aucgcuagcg gccagcggga cuauaaagau     1740

cuugacgcca gaauccucca auucauuuuu gaucgcguca aggcuuccga cgagcugcug     1800

cucaaugaaa ucuacuucca ggccaaaaag cugaagcaga aagccucuag cgagcuugag     1860

aagcuggaau ccagcaaaaa gcucgaugag gugauugcua acucucaacu gucccagauc     1920

cugaaaagcc agcauacgaa uggcauuuuc gaacagggga ccuuucuuca ccucguuugc     1980

aaguauuaca agcaacggca gagagcgcgc gacucucggc uguauaucau gcccgaguac     2040

agauaugaua aaaagcugca uaaauacaac aauacaggac gcuucgacga ugacaaccag     2100

cuccugaccu acuguaauca caagccgcgg cagaaaagau aucaacuucu gaacgaucuc     2160

gccggcgugc ugcaggucuc cccuaauuuu cugaaggaca aaauugggag cgaugacgac     2220

cuuuucaucu ccaaguggcu cguggaacac auccgcggau uuaagaaggc uugcgaggau     2280

ucucugaaaa uucagaaaga caaccggggc cugcucaacc auaagaucaa uauugccaga     2340

aacacaaaag ggaaguguga gaaggaaauc uucaaucuga uuugcaaaau cgagggaagc     2400

gaagauaaga aaggcaacua caagcacggc cuugccuaug agcugggggu ucuccuguuu     2460

ggagaaccca augaggcuuc caaaccugag uucgaccgca agaucaagaa auuuaacucu     2520

auuuacagcu ucgcccagau ccaacagauu gcguuugcug aacggaaggg caaugccaac     2580

accugugccg ugugcuccgc ugauaaugcc cauagaaugc agcaaaucaa aauuacggag     2640

cccgucgaag acaacaagga uaaaaucauc cugagcgcua aggcccagcg gcuucccgcg     2700

auuccuacac gcaucgugga cggggccguc aagaaaaugg cuaccauucu ggccaagaau     2760

aucguggaug acaacuggca gaauauuaaa cagguucucu cugcuaagca ccaacugcac     2820

aucccgauca uuaccgaguc caacgccuuc gaauuugagc ccgcgcucgc cgaugugaaa     2880

ggaaagagcc ugaaggacag acggaaaaag gcucuugaga gaaucucucc ugaaaauauu     2940

uucaaagaca agaacaaccg caucaaagag uucgccaagg gcauuuccgc uuauagcggg     3000

gccaaucuga cagauggcga cuuugaugga gccaaagagg aacucgacca uaucauuccc     3060

cggucucaca agaaguacgg cacccugaac gaugaggcua aucugaucug cgucacaaga     3120

ggggacaaca aaaauaaggg aaaccgcauc uucugucuua gagaucuggc ggacaauuau     3180

aaacucaagc aguuugaaac gaccgaugac cuggagauug agaaaaagau cgccgauaca     3240

auuugggacg cuaacaagaa agauuucaag uuuggcaauu accggagcuu caucaaccuc     3300

accccucagg aacagaaagc cuuucgccau gcccuguucc uugcugacga aaaucccauu     3360

aagcaagccg ugaucagagc gaucaacaau cggaaccgca cauuuguuaa ugggacccag     3420

agauacuucg cugaggugcu ggccaacaau auuuaucucc gggccaaaaa ggagaaccug     3480

aacacggaca agaucuccuu ugauuacuuc ggcauuccga ccaucggaaa ugggcgcggc     3540

auugcugaaa ucagacagcu guaugagaaa gucgacagcg auauccaggc cuacgcgaag     3600

ggagacaaac cucaggcuuc uuauucccac cuuauugaug ccaugcuggc cuucugcauc     3660

gcugccgacg aacaucggaa cgauggcagc auugggcucg agaucgacaa gaauuacucc     3720

cuguaucccc ucgauaaaaa cacaggagaa guguuuacca aggacauuuu cucucaaauc     3780

aagauuacag auaaugaguu uagcgacaaa aagcuggugc gcaaaaaggc uaucgagggc     3840

uucaacaccc acagacagau gacgcgggau gggaucuacg ccgaaaauua ccuuccuauu     3900

cugauccaca aagagcugaa cgaaguuaga aagggauaua cauggaaaaa uuccgaggaa     3960

auuaagaucu uuaagggcaa aaaguacgac auucagcaac ucaacaaucu ggucuauugu     4020

cuuaaauucg uggacaagcc caucucuauc gauauucaga ucagcacccu ggaggagcuc     4080

cgcaacauuc ugacaaccaa uaacaucgcg gccaccgcug aauacuauua cauuaaucuc     4140

aaaacacaga agcugcauga guauuacauc gaaaacuaca acacggcccu uggguauaag     4200

aaauacucca aggagaugga guuucugcgg ucucuggcuu auagaagcga acgcgugaaa     4260

aucaagucca uugacgaugu caaacaagug cucgacaagg auagcaauuu caucauuggc     4320

aagaucaccc ugccuuuuaa aaaggagugg cagcggcuuu acagagaaug gcagaacaca     4380

accauuaaag acgauuauga guuccugaag ucuuucuuca auguuaaauc caucacaaag     4440

cuccacaaga aagugcgcaa ggacuuuagc cugcccauuu cuaccaacga aggaaaauuc     4500

cucgucaagc ggaaaacgug ggauaauaac uucaucuacc agauccugaa ugacuccgau     4560

agcagagccg acggcacaaa gccguuuauu ccugccuucg acaucucuaa aaacgagauu     4620

guggaggcua ucauugauuc cuuuaccagc aagaauaucu ucuggcuucc caagaacauc     4680

gaacugcaaa aaguugacaa uaagaacauu uuugcgaucg auaccuccaa augguucgag     4740

guggaaacac ccagcgaccu gcgcgauauu gggaucgcca ccauucagua uaagaucgac     4800

aauaacucuc ggccuaaagu cagagugaag cucgauuacg uuaucgacga ugacagcaag     4860

auuaauuacu uuaugaacca uucccugcuu aaaucucggu auccggauaa ggugcuggag     4920

auccucaaac agagcacaau uaucgaauuu gaguccucug gauuuaacaa gacgauuaaa     4980

gagaugcugg gcaugaagcu cgcugggauc uacaaugaaa ccagcaacaa uaagcgcccc     5040

gccgccacca aaaaggcugg acaggccaaa aagaaaaagu aaggacuagu cccuguuccc     5100

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     5160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     5220

uu                                                                    5222


<210>  4700
<211>  3494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4700
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaag cggaacuaca uucugggacu cgacaucggc      180

auuaccagcg uuggguaugg aaucauugau uacgagacac gcgacgugau cgaugcgggc      240

gugagacugu ucaaagaagc uaaugucgag aacaaugaag ggcggcgcuc caagagagga      300

gcccggcgcc uuaagagacg gcgcagacau cggauccagc gcgugaaaaa gcugcucuuu      360

gacuauaacc ugcuuacgga ucacucugag cugagcggca uuaauccgua cgaagccaga      420

guuaaaggcc ucucccaaaa gcugucugag gaggaguuca gcgcugcccu gcuccaucug      480

gcuaaacggc gcggggugca caacgucaau gaaguggaag aggacaccgg aaacgagcuu      540

uccacaaagg aacagaucuc uagaaauagc aaggcccugg aggaaaaaua uguugcggag      600

cuccagcugg aacggcuuaa gaaagauggc gaggugagag gguccauuaa ccgcuuuaag      660

accagcgacu acgucaaaga ggccaagcag cugcucaaag ugcaaaaggc uuaucaucag      720

cuggaucagu cuuucaucga cacauacauu gaucugcucg aaacccggag aacauacuau      780

gagggacccg gcgaaggguc cccuuuugga uggaaggaca ucaaagagug guacgaaaug      840

cugaugggcc acugcaccua uuuccccgag gagcuucgca gcgugaagua cgccuauaau      900

gcugaucugu acaacgcccu caaugaccug aacaaucuug ucauuacgcg ggacgaaaac      960

gagaaacugg aauauuacga gaaguuucag aucaucgaaa acguuuucaa acaaaagaag     1020

aaaccuaccc ucaagcagau ugcgaaagag auccugguga augaggaaga uauuaagggc     1080

uacagaguga caucuaccgg gaaacccgag uuuacaaacc ugaaggucua ucacgacauc     1140

aaggauauua ccgcccgcaa agaaaucauc gagaaugcug agcuccugga ccagauugcc     1200

aagauccuua cgauuuacca guccagcgaa gauauccaag aggaacugac aaaccucaau     1260

ucugagcuga cccaggaaga gauugagcag aucuccaacc uuaaaggaua uacaggcacc     1320

cauaaucuga gccucaaggc uaucaaccug auucuggacg aacucuggca caccaaugau     1380

aaccaaaucg ccauuuucaa ucggcugaaa cuugugccua agaagguuga ccugucccag     1440

cagaaagaga ucccgacaac gcucguggau gacuuuauuc ugucucccgu cgugaagcgc     1500

agcuucaucc aguccauuaa aguuaucaac gccaucauua agaaauacgg gcugccuaau     1560

gauaucauua ucgaacuugc ucgcgagaag aacagcaaag acgcgcaaaa gaugauuaau     1620

gaaaugcaga agcggaacag acagaccaac gagcgcaucg aggaaaucau ucggacaacc     1680

ggaaaagaga augccaagua ucucaucgag aaaauuaagc ugcaugacau gcaggaaggc     1740

aaaugucugu acucucucga agcuaucccc cuggaggauc uucugaacaa uccuuuuaac     1800

uaugaggugg accacauuau ccccagaucc gucagcuucg auaauucuuu caacaauaag     1860

gugcucguca agcaagaaga gaacagcaaa aaggggaauc ggacaccguu ucaguaccug     1920

uccucuagcg acuccaaaau cagcuacgaa accuucaaga aacacauucu gaaccuugcc     1980

aagggaaagg gccgcaucuc uaaaacgaag aaagaguauc uccuggagga aagagauauu     2040

aaucgguuuu ccgugcagaa ggacuucauc aacagaaauc ugguugauac acgcuacgcc     2100

acccggggcc ucaugaaccu gcuuagaagc uauuuucgcg ugaauaaccu ggacgucaaa     2160

gugaagucua uuaacggggg auucaccucc uuucucagac ggaaguggaa auucaagaaa     2220

gagcgcaaua agggcuacaa acaucacgcu gaagaugccc ugaucaucgc gaacgcugac     2280

uuuauuuuca aggaguggaa aaagcuggau aaggccaaaa agguuaugga aaaucagaug     2340

uuugaggaga aacaagccga aagcaugccu gagaucgaaa cagagcagga auauaaggag     2400

auuuucauca caccccauca gauuaaacac aucaaggacu uuaaggauua caaauauucc     2460

cauagagugg acaagaaacc uaaccgggag cuuauuaaug acacccucua cucuacgcgc     2520

aaggaugaca aagggaacac ccugaucguc aauaaccuga auggacucua cgauaaggac     2580

aacgauaagc ugaagaaacu uaucaauaaa agccccgaaa agcugcucau guaucaccac     2640

gacccccaga cauaccaaaa acugaagcug auuauggagc aguauggcga ugaaaagaac     2700

ccucuuuaca aauauuacga ggaaaccggg aauuaucuca caaaguacuc caaaaaggac     2760

aacggcccgg ugaucaaaaa gauuaaguac uauggaaaua aacugaacgc ucaucuggau     2820

aucaccgacg auuaccccaa cucuagaaau aaggugguua aacucagccu gaagccuuau     2880

cgguucgacg ucuaccuuga uaacggcgug uauaaauucg ugacggucaa gaaucuggac     2940

gugauuaaaa aggagaacua cuaugagguu aauuccaagu gcuacgaaga ggccaaaaag     3000

cucaaaaaga ucagcaacca ggcugaauuu aucgccucuu ucuacaauaa cgaccugauu     3060

aaaaucaaug gggagcugua ucgcgugauu ggagucaaca augaucuucu gaacagaauc     3120

gaggugaaua ugauugacau caccuaccgg gaauaucucg agaacaugaa ugauaagaga     3180

cccccuagaa ucauuaagac aaucgcgucc aaaacccaaa gcauuaagaa auacucuaca     3240

gacauccugg gcaaccucua ugaaguuaag uccaaaaagc acccccagau uaucaagaaa     3300

gggaagcggc cggccgcuac caaaaaggcc ggacaggcua aaaagaagaa auaaggacua     3360

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     3420

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaag aauu                                                       3494


<210>  4701
<211>  4562
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4701
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcccug uucaacaagu gcauuaucau uagcaucaau      180

cucgacuuuu ccaacaaaga gaaguguaug accaagccgu acucuauugg acuggauauc      240

ggcacaaaua gcguugggug ggcggugauc acggacaacu auaaagugcc cuccaagaaa      300

augaaggucc uuggaaauac cucuaaaaag uacauuaaga aaaaccugcu cggcgugcug      360

cuuuucgaua gcgggaucac agcugaagga cggcgccuga agagaaccgc ccggcgcaga      420

uauacacggc gcagaaaucg gauucucuac cugcaggaga ucuuuuccac cgaaauggcc      480

acacuggacg augcuuucuu ucaacgccuc gacgauucuu uccugguucc ugacgauaaa      540

agagacagca aguaucccau uuuuggcaac cuuguggagg aaaaagucua ccaugaugag      600

uucccuacca ucuaucaucu gcggaaguac cucgccgacu ccacgaaaaa ggcugaucug      660

cgccuugugu accuggcccu cgcgcacaug auuaaguaua gaggccacuu ucugaucgag      720

ggggaguuca auagcaaaaa caaugacauc cagaagaacu uucaggacuu ccuggauacc      780

uacaaugcca uuuuugaauc ugaccucucc cuggaaaaca gcaaacagcu ugaggagauc      840

guuaaggaua aaauuucuaa gcuggaaaag aaagaccgga uccucaagcu guuccccgga      900

gagaaaaauu ccggcauuuu cagcgaauuu cuuaagcuga ucguggggaa ccaagcugau      960

uuccgcaaau gcuuuaaccu cgacgagaag gccucucugc auuucuccaa ggaaagcuau     1020

gaugaggacc uggagacacu ccugggauac aucggcgaug acuauuccga ugucuuucuu     1080

aaagcuaaga aacuguacga cgccauucuc cugucugggu uccuuaccgu gacagacaau     1140

gaaaccgagg cgccucugag cuccgccaug aucaagagau auaacgaaca caaagaggau     1200

cucgcucugc ugaaggaaua cauucggaau aucagccuca agacguacaa cgagguguuu     1260

aaagacgaua caaagaaugg auaugccggc uacauugacg gcaaaaccaa ccaggaggau     1320

uucuaugucu accugaagaa ucuucuggcu gaauuugagg gggccgacua uuuccucgaa     1380

aagaucgaua gagaggacuu ucugcgcaag cagcggacau ucgauaacgg aucuaucccg     1440

uaccagauuc aucugcaaga aaugagagcc auccuugaca aacaggcuaa guuuuauccc     1500

uuccucgcga agaauaaaga gcgcauugag aagauccuga ccuuccggau uccuuacuac     1560

guuggccccc uggccagagg gaacuccgau uuugcuugga gcauccgcaa acggaaugaa     1620

aagauuaccc cuuggaacuu cgaggacgug aucgauaaag aaucuagcgc cgaggccuuu     1680

aucaauagaa ugacauccuu cgaccucuau cugcccgagg aaaaggugcu uccgaagcac     1740

ucucugcucu acgagacguu uaacgucuau aaugaacuga ccaaagugcg cuucauugcu     1800

gagagcaugc gggacuacca guuucuggau uccaagcaga aaaaggacau cguuagacuu     1860

uauuucaaag auaagcgcaa ggugacagac aaagauauua ucgaauaccu ccacgccauu     1920

uauggauacg acggcaucga gcugaagggg aucgagaaac aauuuaacag cucucugucc     1980

accuaccaug aucuccugaa cauuaucaau gacaaggagu uccuugauga cagcucuaac     2040

gaagcgauua ucgaagagau uauccacaca cugaccaucu uugaagaucg ggagaugauu     2100

aaacagagac ucuccaaguu cgagaauauc uuugacaaga gcguccugaa aaagcugucc     2160

cggcgccacu auacgggaug gggcaaacuu ucugcuaagc ucauuaacgg caucagagau     2220

gaaaagagcg ggaauacaau ucuggacuac cugaucgacg auggaaucuc caaccggaau     2280

uucaugcagc ucauucauga cgaugcccug ucuuucaaga aaaagaucca aaaggcccag     2340

auuaucggcg acgaggauaa agggaacauu aaggaagugg uuaaaagccu uccuggaucc     2400

cccgcuauca agaaaggcau ucugcagagc aucaagaucg uggacgagcu cgucaaggug     2460

augggcggga gaaaaccuga aucuauuguc guggagaugg cccgcgagaa ucaguauacc     2520

aaccaaggaa aguccaauag ccagcagcgg cugaaaagac uggaaaaguc ucuuaaagag     2580

cucggcucca agauccugaa ggaaaacauu cccgcuaaac ugagcaagau cgauaauaac     2640

gcccuccaga augaccgccu guaccuuuau uaccugcaaa acgggaaaga uauguauacc     2700

ggagacgauc ucgacauuga cagacugucu aacuacgaua ucgaccacau cauuccccag     2760

gcguuucuga aggauaauag caucgacaac aaaguucuug uguccagcgc cucuaaucgg     2820

ggcaaguccg augacuuccc uagccuggag gucgugaaga aacgcaagac auuuugguac     2880

cagcuccuga aauccaagcu cauuucucag agaaaauucg auaaccugac caaggcugag     2940

cgcgggggac uucugccgga agacaaggcc ggcuuuaucc aacggcagcu cguugagaca     3000

agacagauua cgaaacaugu ggcucggcug cuggaugaaa aguucaauaa caaaaaggac     3060

gagaauaacc gcgccgucag aaccgugaaa aucaucacac uuaagagcac ccuggugucc     3120

caguuucgga aagauuucga acucuauaag guucgcgaga uuaaugacuu ucaccaugcg     3180

cacgaugccu accugaacgc ugucaucgcc ucugcucucc ugaagaaaua ucccaaacuu     3240

gagccugagu ucguguacgg ggacuauccc aaauacaaua gcuuuagaga acggaagucc     3300

gccacagaaa aaguguauuu cuacucuaac auuaugaaua ucuucaaaaa gagcauuucc     3360

cuggccgacg gcagagucau cgagcgcccu cucauugaag ugaacgagga gaccggagaa     3420

agcguuugga auaaagaguc ugaucuggcu acggugcgga gaguccuguc cuacccccaa     3480

gugaacguug ugaagaaagu cgaggaacag aaccacggcc uugaccgcgg gaagccgaaa     3540

ggacuguuua augcgaaccu cagcucuaag ccuaagccca auuccaacga aaaucuggug     3600

ggcgccaaag aguaucucga uccuaagaaa uacgggggau augcuggcau cagcaacucu     3660

uucgccguuc uggugaaggg gaccaucgag aaaggcgcca agaagaaaau uacaaauguc     3720

cuugaauuuc agggaaucuc cauucuggac cggaucaacu acagaaagga uaaacugaau     3780

uuccuccugg agaagggcua uaaagacauu gaacuuauca ucgagcugcc caaguacagc     3840

cucuuugagc uguccgaugg gagccgccgg augcucgcuu cuauucugag caccaacaau     3900

aaaagaggag aaauccauaa gggcaaccaa auuuuccuuu cccagaaguu ugugaaacug     3960

cuguaucacg ccaagcgcau cucuaauaca auuaacgaga aucaucggaa auacguggaa     4020

aaccacaaga aagaguucga ggaacucuuu uacuauaucc uggaguucaa ugaaaacuac     4080

gucggggcga agaagaacgg aaaacuucug aauagcgcuu uucaguccug gcagaaccac     4140

ucuaucgacg agcucuguag cuccuucauu ggcccuaccg ggucugaaag aaagggccug     4200

uuugagcuca cgagccgggg auccgccgcc gauuucgagu uccugggcgu uaaaaucccc     4260

cgcuauagag acuacacacc gagcucucuu cugaaggaug cuacccugau ucaucaaucc     4320

gugacagggc ucuaugaaac ccggaucgac cuggccaaac uuggagaggg caagcgcccu     4380

gcggcuacca agaaagccgg gcaggccaag aaaaagaaau aaggacuagu cccuguuccc     4440

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     4560

uu                                                                    4562


<210>  4702
<211>  3638
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4702
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccugg uacgcgagcc ugauguccgc ucaucaccuc      180

cggguuggaa uugacguggg cacccauucu guggggcugg ccacacuucg cgucgaugac      240

cacggaacgc cgaucgagcu gcucagcgcc cugucccaua uucacgauuc uggcgugggg      300

aaggaaggga aaaaggacca cgauaccaga aagaaacuua gcggcaucgc ucggcgcgcc      360

agacggcugc uccaucaccg cagaacacag cugcaacagc uggacgaggu ucuccgggau      420

cugggcuucc ccauuccuac ccccggggaa uuucuugacc ugaacgagca gacagauccu      480

uaucgcgugu ggagaguccg ggcucgccuc guggaagaga agcugcccga ggaacuuaga      540

ggaccugcca ucuccauggc gguucggcau aucgcccgcc acagaggcug gcggaauccg      600

uacucuaaag uggagagccu gcucuccccc gcugaagaga gcccuuucau gaaggcccug      660

agagagcgca uucuggcuac cacaggggaa guccucgacg auggaaucac ccccggccag      720

gccauggcgc aaguggcccu gacgcacaac auuucuaugc gggggccuga gggaauccuu      780

ggcaaacugc aucaguccga caaugcuaac gaaauuagaa agaucugcgc ccgccagggc      840

gugagccccg augucuguaa gcagcuccug cgggcuguuu uuaaagccga cucuccgaga      900

ggguccgccg ugagccgcgu ggcuccugac ccccuuccug gacaaggcuc uuuccggaga      960

gcgcccaagu gcgaucccga guuucagcgc uuccggauua ucuccaucgu cgccaaucug     1020

agaauuagcg aaaccaaagg ggagaaccgc ccucucacag cugacgagcg gagacacgug     1080

guuaccuuuc ugacagaaga uucccaggcc gaccugaccu ggguggaugu cgccgagaag     1140

cucggagugc aucggcgcga ccugagaggc acggcuguuc acacagauga cggggaacgg     1200

ucugccgcga gaccgcccau cgaugcuacc gaccgcauua ugcggcagac aaaaaucagc     1260

ucccuuaaga ccugguggga ggaagccgau agcgagcaaa gaggagccau gauucgcuau     1320

cuguacgagg acccuaccga cucugaaugu gcugagauca ucgccgaacu ccccgaggaa     1380

gaucaggcua aacuggacuc ccuucaucug ccugccggca gagcggccua uagccgggag     1440

ucucucacag cucugagcga ucacaugcug gccacgaccg acgaucucca cgaggcucgc     1500

aagagacugu ucggcgugga cgauuccugg gcgcccccgg ccgaagccau uaaugcuccu     1560

gucgggaacc ccucugugga ccggacacuu aagaucgucg gacgcuaccu gagcgccgug     1620

gaguccaugu ggggcacccc ugaaguuauu cauguggagc acgucagaga uggguuuaca     1680

agcgagcgga uggcugacga acgcgauaaa gccaauagac ggcgcuauaa cgacaaucag     1740

gaggccauga agaaaaucca aagagauuac ggaaaggaag gcuacauuuc ucggggagac     1800

aucgugagac ucgacgcucu ggagcugcag ggcugcgcgu gucuuuauug cggaaccacg     1860

aucggcuacc auacauguca gcucgaucac auuguucccc aggccgggcc uggauccaac     1920

aaucgccggg gcaaccuggu ggcugucugc gaaagaugua aucgcagcaa aucuaacacc     1980

cccuucgccg ugugggccca aaagugcggg aucccgcacg ugggaguuaa ggaggcuauu     2040

ggccggguca gaggguggcg caaacagacc ccuaacacau ccagcgagga ccugacccgg     2100

cucaagaaag aagugaucgc cagacugcgc cggacacagg aggaucccga aauugacgag     2160

agauccaugg aaucuguggc guggauggcu aaugagcuuc aucaccggau cgccgccgcu     2220

uaucccgaga cgaccgucau gguguaccgc ggcagcauua cagccgcggc uagaaaggcc     2280

gccggaaucg auucccggau caaccugauu ggcgaaaaag ggcgcaagga cagaaucgau     2340

cggagacauc acgcuguuga cgccucugug gucgcucuca uggaggccag cguggcgaag     2400

acccuggccg aacgcuccag ccugcgggga gagcagagac uuacaggcaa agaacaaacc     2460

uggaagcagu auacgggguc uaccguugga gcucgcgagc auuuugagau guggcggggc     2520

cacaugcucc accugacaga acuguucaau gagagacucg ccgaagauaa aguguacguc     2580

acccagaaca uucgccugcg gcuuuccgac gggaaugcuc auacagugaa cccuagcaag     2640

cugguuucuc acagacucgg cgauggacug accgugcagc aaaucgaccg cgccuguacg     2700

ccggcccugu ggugcgcucu uacaagagag aaagauuuug acgagaagaa uggccucccc     2760

gcgcgggaag accgcgccau uagaguccau gggcacgaga ucaaguccag cgauuauauc     2820

cagguguucu cuaaacggaa gaaaaccgac agcgaucgcg acgaaacacc uuucggagcu     2880

auugccguga gaggcggguu ugucgagauc ggacccucca uucaucacgc ccggaucuac     2940

agaguugaag gcaagaaacc uguguacgcu augcugcgcg uguucaccca cgaucugcuc     3000

agccagcggc auggggaccu guuuucugcc gucauucccc cucaguccau cagcaugaga     3060

ugcgcggagc ccaaacuucg caaagcuauc accacaggca acgccacgua ucugggaugg     3120

gugguugugg gcgaugagcu cgaaauuaau gucgacuccu ucaccaagua cgccaucggg     3180

cgguuucugg aggauuuccc gaacacaacc agauggcgga uuuguggaua ugacacaaau     3240

ucuaagcuga cccuuaaacc uaucgugcuc gcugccgaag gccuggagaa ccccagcucc     3300

gcgguuaaug aaauugugga gcugaagggg uggcgcgucg cuaucaacgu gcucacgaaa     3360

guucacccua caguggucag acgggaugcc cugggacgcc ccagauacuc uagccggucc     3420

aaucuuccga ccucuuggac caucgagaag cgcccugccg cuacaaagaa ggccggccaa     3480

gcuaagaaaa agaaauaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu     3540

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                             3638


<210>  4703
<211>  4499
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4703
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccacc aagccguaca gcauuggacu ggacaucggc      180

acaaacuccg uugggugggc ggugacgacc gauaauuaua aagugcccuc uaagaagaug      240

aaaguccucg gaaacacaag caagaaauac auuaagaaaa aucugcuugg cgugcuccug      300

uucgacuccg ggaucaccgc ugagggacgg cgccugaaga gaacagcccg gcgcagauau      360

acccggcgca gaaaccggau ucuuuaccug caggaaaucu uuucuacaga gauggccacc      420

cucgaugacg cuuucuuuca acgccuggau gacagcuucc ugguuccuga ugacaagaga      480

gauuccaaau aucccaucuu uggcaaucuc guggaagaga aggccuacca ugacgaguuc      540

ccuacgauuu aucaucugcg gaaauaccuu gcugauucua ccaagaaagc cgaccugcgc      600

cucgucuacc uggcgcuugc ccacaugauc aaguauagag gccacuuucu gauugagggg      660

gaguucaaca gcaaaaauaa cgauauccag aagaauuuuc aggacuuccu cgacacauac      720

aacgcuauuu uugaauccga ucugagccug gaaaauucua agcagcucga agagaucgug      780

aaagacaaga uuuccaaacu ggagaagaaa gaucggaucc uuaagcuguu ccccggagaa      840

aagaacagcg gcaucuuuuc ugaguuccuc aaacugauug uugggaauca agccgacuuc      900

cgcaagugcu uuaaccuuga ugaaaaggcu ucccugcauu ucagcaaaga gucuuaugac      960

gaagaucucg agacccugcu gggauacauc ggcgacgauu auuccgacgu guuucucaaa     1020

gccaagaagc uguacgaugc gauucuucug agcggguucc ucacagucac cgacaaugag     1080

acggaagccc cucuguccuc ugcuaugauc aaaagauaua acgagcacaa ggaagaccuu     1140

gcccugcuca aagaguacau ucggaacauc agccugaaga cauacaauga aguguuuaaa     1200

gaugacacca agaacggaua ugcuggcuac aucgauggca agacaaauca ggaggacuuc     1260

uauguguacc ugaaaaagcu ccuggccgag uuugaagggg ccgauuauuu ccuugagaaa     1320

auugacagag aagauuuucu gcgcaagcag cggaccuucg acaacggauc caucccguac     1380

cagauucacc uccaagagau gagagcuauc cuggauaaac aggcgaaguu uuaucccuuc     1440

cuggccaaaa auaaggaacg cauugagaag auccuuaccu uucggauccc uuacuacguc     1500

ggcccccucg cuagagggaa cagcgacuuc gccuggucua uucgcaaacg gaaugagaag     1560

aucacaccuu ggaacuucga agauguuauu gacaaagagu ccagcgccga ggcuuuuauc     1620

aauagaauga cgucuuucga ucuguaucug cccgaagaga aggugcuccc gaaacauagc     1680

cugcuuuacg aaaccuuuaa cguguauaau gagcugacaa agguccgcuu cauugccgaa     1740

uccaugcggg acuaccaguu ucucgacucu aagcagaaaa aggauaucgu gagacuguau     1800

uucaaagaca agcgcaaagu uaccgauaag gacauuaucg aguaccugca cgcgaucuau     1860

ggauacgaug gcauugaacu uaaggggauc gagaaacaau uuaacagcuc ccucagcaca     1920

uaccaugacc ugcugaauau uaucaacgau aaggaguucc ucgacgauuc uuccaaugaa     1980

gcuauuaucg aggaaaucau ucacacccug acgaucuuug aggaccggga aaugauuaaa     2040

cagagacuua gcaaguucga gaacaucuuu gauaaaucug ugcugaagaa gcucucccgg     2100

cgccacuaua caggaugggg caaacugagc gccaagcuga uuaacggcau cagagacgag     2160

aaauccggga auaccauccu ugauuaccuc auugacgacg gaaucucuaa ccggaauuuc     2220

augcagcuga uucaugauga cgcccugagc uuuaagaaaa agauccaaaa agcucagauu     2280

aucggcgaug aagacaaggg gaacaucaag gaggucguga aaucccuccc uggaucuccc     2340

gccauuaaga aaggcauccu gcagagcauu aagaucguug augaacuugu gaaagucaug     2400

ggcgggagaa agccugaguc cauugugguc gaaauggcuc gcgagaauca guauaccaac     2460

caaggaaaga gcaauucuca gcagcggcug aaaagacucg agaagucccu gaaagaacug     2520

ggcagcaaga uccuuaaaga gaacauuccc gccaagcucu cuaagaucga caauaacgcg     2580

cugcagaaug aucgccugua ccucuauuac cugcaaaacg ggaaagacau guauacagga     2640

gaugaccuug auaucgacag acuguccaau uacgacauug aucacaucau uccccaggcc     2700

uuccucaagg acaacagcau cgauaauaaa gugcugguuu cuagcgcuuc caaccggggc     2760

aagagcgacg augugccuuc ucuggaaguc gugaaaaagc gcaagaccuu cugguaccag     2820

cuucugaaau ccaagcucau uagccagaga aaauuugaca accugacaaa ggccgagcgc     2880

gggggacucu ccccggagga uaaagcuggc uucauccaac ggcagcuggu ugaaacgaga     2940

cagaucacca agcauguggc ccggcuucug gacgagaagu uuaauaacaa aaaggaugaa     3000

aauaaccgcg cggucagaac agugaaaauu aucacccuca agucuacacu ggugagccag     3060

uuccggaaag acuuugagcu guauaagguu cgcgaaauua augauuucca ccaugcccac     3120

gacgcuuacc uuaacgccgu cguggcuucc gcccugcuca aaaaguaucc caagcuggag     3180

ccugaguuug uguacgggga uuaucccaaa uacaauucuu ucagagaaag aaagagcgcc     3240

accgagaaag ucuauuuuua cuccaacauc augaauauuu ucaagaaauc uaucagccuc     3300

gcugacggcc gggugaucga acgcccucug auugagguua acgaagagac gggagagucc     3360

guguggaaua aggaaagcga ccuugcgacc guccggagag ugcugucuua cccccaaguu     3420

aacgugguca agaaagugga ggaacagaau cacggccucg aucgcgggaa gccgaaagga     3480

cuguuuaacg ccaaucuguc cagcaagccu aaacccaacu cuaacgagaa ucuuguuggc     3540

gcuaaggaau aucuggaccc uaagaaauac gggggauaug ccggcaucuc caacagcuuc     3600

acagugcucg ucaaggggac cauugagaaa ggcgccaaga aaaagaucac aaaugugcug     3660

gaguuccagg gaauuucuau ccucgaucgg auuaacuaca gaaaggacaa acugaauuuu     3720

cuucuggaaa agggcuauaa agauaucgag cugaucauug aacuccccaa guacucccug     3780

uucgagcuua gcgacggguc ccgccggaug cuggcuagca uccucucuac caacaauaaa     3840

agaggagaga uucauaaggg caaccaaauc uuucugagcc agaaauucgu gaagcuccug     3900

uaucacgcca agcgcauuuc caauacgauc aacgaaaauc aucggaaaua cgucgagaac     3960

cacaagaaag aauuugagga acuuuucuac uauauccugg aguuuaauga gaacuacguu     4020

ggggcgaaga aaaauggaaa gcugcucaac ucugcuuucc agagcuggca gaaucacucc     4080

auugaugaac uguguucuag cuuuaucggc ccuacagggu ccgagagaaa gggccuuuuc     4140

gaacugaccu cucggggaag cgccgccgac uuugaguucc ucggcgugaa aauuccccgc     4200

uauagagauu acacaccguc cagccugcuc aaggacgcua cccugaucca ucaaucugug     4260

accgggcuuu augaaacacg gauugaucug gccaaacugg gagagggcaa gcgcccugcg     4320

gcuacgaaaa aggccgggca ggccaagaaa aagaaauaag gacuaguccc uguucccaga     4380

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      4499


<210>  4704
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4704
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccgcg gcuuucaagc cgaacagcau uaauuacauc      180

cugggacucg acauuggcau cgccuccguu gggugggcca ugguggagau ugaugaagag      240

gaaaacccca uccggcugau cgaccuugga gugcgcgucu uugagagagc ugaagugccu      300

aaaaccggcg auucucuggc cauggcucgg cgccucgcca gaagcguucg gcgccugaca      360

agacggcgcg cgcauagacu ucugcggacg cgcagacucc ugaagcggga gggggugcug      420

caggccgcua auuucgacga gaacggacuc auuaaguccc ugcccaauac cccuuggcaa      480

cuucgcgccg cugcccugga uagaaaacuc acaccccugg aauggucugc gguccuucug      540

caccucauca agcaucgggg cuaucugagc cagcgcaaaa acgagggcga aaccgccgac      600

aaggagcugg gggcucuccu gaaaggagug gccggcaaug cucacgcccu ucagacaggg      660

gauuuuagaa ccccugccga gcuggcucuc aacaaguucg aaaaggaguc cggacauauu      720

cggaaucaga gaucugacua cagccacaca uuuucccgca aagaucugca agcggaacuu      780

auccuccugu ucgagaagca gaaagaauuu ggcaacccgc acguuagcgg gggacugaag      840

gagggcauug agacccugcu caugacgcag cggcccgccc ugucuggcga cgcugugcag      900

aaaaugcuug ggcauugcac cuucgaaccu gccgagccca aggccgcuaa aaauacauau      960

accgccgaaa gauuuaucug gcugacaaag cucaacaauc ugcgcauucu ugagcaagga     1020

uccgaacggc cucugaccga uacggagaga gcgacacuca uggacgagcc cuaccgcaag     1080

agcaaacuga ccuaugcuca ggcccggaag cugcucggcc uggaagauac agccuucuuu     1140

aaagggcuua gauacggaaa ggacaacgcu gaggccucua cccugaugga aaugaaggcu     1200

uaucacgcca ucucccgcgc gcucgagaag gaaggccuga aggacaaaaa gagcccgcug     1260

aaucuuucuc cugagcucca ggaugagauc gggaccgccu ucucccuguu uaaaacagac     1320

gaagauauua cgggacggcu gaaggacaga auccagcccg agauucucga agcucugcuu     1380

aaacauauca gcuucgauaa guucguccaa auuucccuga aggcccuccg gcgcaucgug     1440

ccucugaugg agcagggcaa aagauacgac gaggcuugug ccgaaaucua cggcgaucac     1500

uaugggaaga aaaacaccga ggaaaagauu uaccugccuc ccauccccgc ggacgagauu     1560

cggaacccgg ugguccuuag agcccucucu caggcucgca aaguuaucaa uggaguggug     1620

cggagauaug gcagccccgc ccgcauucac aucgaaacag cuagagaggu cgggaagucc     1680

uuuaaggauc ggaaagagau cgaaaagcgc caagaggaaa acagaaaaga ccgggagaag     1740

gccgccgcua aauuccgcga auacuuuccu aauuucgugg gagagcccaa gagcaaagau     1800

auucugaagc ugagacucua ugagcagcag cauggcaagu gccuguacuc ugggaaagaa     1860

aucaaccuug gccggcugaa ugagaaggga uauguugaaa uugaccacgc gcucccuuuu     1920

ucccgcaccu gggacgauag cuucaacaau aaagugcugg uccugggcuc ugagaaccag     1980

aauaagggga accaaacacc cuacgaauac uuuaauggaa aagacaacag cagagagugg     2040

caggaguuca aggcccgggu ggaaaccucc cgcuuuccga gaucuaagaa acagcggauc     2100

cuucuccaga aguucgauga ggacggcuuu aaagaaagaa aucugaacga uacgcgcuau     2160

guuaaucggu uccuguguca auuuguggcu gacagaaugc gccucacagg gaagggcaaa     2220

aagcgggucu ucgccagcaa cggacagauu accaaccugc uuagaggguu cuggggccug     2280

cgcaaggugc gggccgagaa ugauagacau cacgcucucg acgccgucgu gguugcgugc     2340

uccaccgugg cuaugcagca gaaaaucaca cgcuuugucc gguacaagga gaugaacgcc     2400

uucgauggaa aaaccauuga caaggaaaca ggcgaggugc ugcaucagaa aacgcacuuu     2460

ccucagcccu gggaguucuu ugcccaggaa guuaugauca gaguguucgg gaagccugau     2520

ggaaagcccg aauuugagga ggcugacacc cuggaaaaac uucggacacu ccuggccgag     2580

aagcugagcu cucgcccuga agcgguccac gaguauguga caccccucuu cguguccaga     2640

gcuccgaauc ggaaaaugag cggccagggg cauauggaaa ccguuaaguc ugccaaacgc     2700

cuggaugagg gagucuccgu gcuuagagug ccucugaccc aacucaagcu gaaagaccug     2760

gagaagaugg ucaaccggga aagagagccc aaacuuuacg aagcccugaa agcucgccuc     2820

gaggcccaca aggacgaucc cgcuaaagcc uuugcggaac cuuucuauaa guacgacaaa     2880

gccggcaauc ggacgcaaca ggugaaggcu guuagagugg agcaagucca gaagaccggg     2940

guguggguuc gcaaccauaa uggcaucgcc gauaacgcua caauggugcg ggucgacgug     3000

uuugagaaag gagauaagua uuaccugguu ccgauuuaca gcuggcaagu ggccaaaggc     3060

auccuccccg acagagccgu cgugcagggg aaggaugaag aggacuggca gcugauugau     3120

gacuccuuca auuuuaaauu cucucuucac ccuaacgauc ugguggaagu caucaccaag     3180

aaggcucgca uguucggaua uuuugcgagc ugucaccggg gcacagggaa uauuaacauc     3240

agaauccaug accucgauca caaaauugga aagaauggca uccuggaggg gauuggcguu     3300

aaaaccgccc uguccuucca gaaguaccag aucgacgagc uuggaaaaga aauucgcccc     3360

ugcagacuga agaagcggcc ucccgugcgc aaaagaccug cugccacgaa gaaagccggc     3420

caggcuaaga aaaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3480

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  4705
<211>  4337
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4705
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaag aaaccguaca ccauuggacu ggacaucggc      180

acaaacagcg uugggugggc ggugcucacg gaucaguaug accuggugaa gcggaagaug      240

aaaauugcug gagauuccga gaagaaacaa aucaagaaaa auuucugggg cguccgccuu      300

uuugacgaag ggcagaccgc cgccgauaga cggauggcuc gcacagccag acggcgcauu      360

gagagacggc gcaacagaau cucuuaccug cagggaaucu ucgcugaaga gaugagcaag      420

accgacgcca auuucuucug ccggcucucc gauucuuuuu auguggacaa cgaaaagcgc      480

aauagcagac aucccuucuu ugcgacaauu gaggaggaag uugaguacca caaaaacuau      540

ccuaccaucu accaucugcg ggaggaacuu gucaauuccu cugagaaggc cgaucugcgc      600

cucgucuauc uggcucuggc ccacauuauc aaauacagag gcaacuuccu cauugaaggc      660

gcucuggaca cacagaauac cagcguggau gggaucuaca agcaauuuau ucagacguau      720

aaccagguuu ucgccuccgg aaucgaggac ggcagccuua aaaagcugga agauaauaaa      780

gacguggcga agauccucgu cgagaaggug acccggaaag aaaagcugga gcgcauucuu      840

aaacuguacc ccggggagaa gucugccgga auguuugcuc aguucaucuc ccucauugug      900

ggcagcaaag ggaacuuuca aaagccuuuc gaccugaucg aaaagucuga uauugagugu      960

gccaaagacu ccuaugaaga ggaucuggaa agccuccugg cucuuaucgg agacgaguac     1020

gccgagcugu ucgucgccgc uaagaaugcg uauucugccg uugugcucuc cagcaucauu     1080

acaguggcug aaaccgagac aaacgccaaa cuguccgccu cuaugaucga aagauuugau     1140

acccaugagg aagaccuugg cgagcugaag gcuuucauua aacuccaucu gcccaagcac     1200

uacgaggaaa ucuuuagcaa uacggagaag cacggcuaug ccggguacau ugauggaaaa     1260

acaaagcagg cggacuucua caaauauaug aagaugaccc uggaaaacau cgagggcgcu     1320

gauuacuuua ucgccaaaau ugagaaggaa aacuuccucc ggaagcagag aacauuugac     1380

aauggggcca ucccucacca gcugcaucuu gaggaacugg aggcuauucu ccaccaacag     1440

gccaaauauu acccguuccu gaaggaaaac uaugauaaaa ucaagucccu ugucaccuuu     1500

cgcauucccu acuucguggg accucuggcu aauggccaga gcgaguuugc cuggcucacc     1560

cggaaagcgg acggggagau cagacccugg aacauugaag agaagguuga cuucggaaaa     1620

ucugccgugg auuuuaucga aaagaugaca aauaaggaca cguaucugcc uaaagagaac     1680

guccugccca agcacucccu cugcuaccaa aaauaccugg uguauaauga acuuaccaag     1740

guucgcuaca ucaacgauca gggcaaaaca agcuauuucu cuggccagga gaaggagcag     1800

auuuucaaug accuguuuaa gcaaaaacgg aaggugaaaa agaaagaucu cgaacuguuc     1860

cugagaaaca ugagccaugu cgaguccccg accaucgaag ggcuugagga cucuuuuaau     1920

agcuccuaca gcacauauca cgaucuccug aaggugggaa uuaagcagga aauccuggac     1980

aacccuguca auaccgagau gcucgagaac auugugaaaa uccugacggu uuucgaagau     2040

aagcgcauga ucaaagagca gcuucagcaa uuuucugacg ugcuggaugg cgucgugcuc     2100

aagaaacugg aacggagaca uuacacaggg uggggacgcc uguccgcuaa gcuccucaug     2160

ggcauucggg acaagcagag ccaccugacc auccuggauu aucucaugaa ugacgacggg     2220

cugaacagaa accuuaugca gcugauuaau gauucuaacc ucuccuucaa aagcaucauu     2280

gagaaggagc agguuaccac agccgacaaa gauauccaau ccaucguggc ugaccuggcc     2340

ggcucucccg cgauuaagaa aggaauccug cagagccuua agauugucga ugaacucgug     2400

uccgugaugg gcuacccucc ccagaccauc guugucgaga uggcccggga aaaucagaca     2460

acggggaaag gaaagaacaa uucucgcccc agauacaaga gccuggagaa agcuauuaag     2520

gaauuuggcu cccaaauccu gaaagagcau ccuaccgaca accaggagcu ccggaauaac     2580

agacuguauc uuuacuaucu gcagaauggg aaggauaugu acacaggaca agaccucgau     2640

auccacaacc ugagcaauua ugacauugau cacaucgugc cgcagucuuu cauuaccgac     2700

aacuccaucg auaaucuggu gcuuacaagc ucugccggca accgcgaaaa aggggacgac     2760

gugcccccuc ucgagauugu gcggaagaga aagguguuuu gggaaaaacu guaccagggc     2820

aaucugaugu ccaagcgcaa auucgauuau cucaccaagg cugagagagg aggccugacg     2880

gaagccgaca aagcccgguu uauccaucgc cagcuuguug agaccagaca aauuacaaag     2940

aacgucgcua acauccugca ccagcgguuc aauuacgaga aggaugacca ugggaacacc     3000

augaaacagg ugcgcaucgu uacacucaag agcgcgcugg ugucucaguu uagaaaacaa     3060

uuccagcugu acaagguccg ggaugugaau gacuaucacc acgcccauga ugcuuaccuu     3120

aacggaguug uggccaauac ccugcucaaa gucuaucccc agcuggaacc ugaguucgug     3180

uacggcgacu aucaccaguu ugauugguuc aaggccaaca aggcuacggc caaaaagcaa     3240

uuuuacacaa auauuaugcu uuucuuugcg cagaaagacc gcaucauuga ugaaaacggg     3300

gagauccugu gggacaagaa auaucuugac accgugaaga aggucaugag cuacagacag     3360

augaauauug uuaaaaagac agaaauccaa aaaggagagu ucuccaaggc uaccaucaaa     3420

cccaagggca acagcucuaa acugauuccg cggaagacca auugggaucc uaugaaguac     3480

gggggccucg acucccccaa cauggccuau gccgugguga ucgaguacgc uaaaggaaag     3540

aauaaacugg ucuuugaaaa gaaaauuauc cgcgugacaa uuauggagag aaaggccuuc     3600

gaaaaggaug agaaagcguu ucuggaggaa cagggcuauc ggcagccuaa gguucuugcu     3660

aaacugccca aguacacgcu cuaugagugu gaagagggga gacgccggau gcuggccagc     3720

gccaacgaag cucagaaagg aaaucaacag gugcucccua accaucuggu cacccuucug     3780

caccaugccg cuaacugcga gguguccgac ggcaagucuc ucgauuacau cgagagcaau     3840

agagaaaugu ucgccgagcu gcuggcgcac guuuccgaau uugccaagcg cuauacacuu     3900

gcugaggcca accugaauaa aaucaaccag cucuuugaac agaauaagga gggggacauu     3960

aaagcuaucg cccaaucuuu cguggaucug auggccuuua acgcuauggg agcgcccgcc     4020

agcuucaagu uuuucgagac cacaauugaa cggaaaagau acaauaaccu caaggagcug     4080

cuuaauucca ccaucauuua ccagucuauc acgggccugu augaaagccg caagcggcug     4140

gacgauaaaa gaccggcugc cacaaagaaa gccgggcagg cuaagaaaaa gaaauaagga     4200

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4260

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aagaauu                                                    4337


<210>  4706
<211>  4370
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4706
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaag aaaccguaca gcauuggacu ggacaucggc      180

accaacuccg uugggugggc gguggugaca gaugacuaua agguccccgc uaagaaaaug      240

aaggugcucg gaaauacgga uaaaucucau auugagaaga accugcuugg cgcccuccug      300

uucgacagcg ggaauaccgc cgaagaucgg cgccugaaaa gaacagcucg gcgcagauac      360

acccggcgca gaaaccggau ccuuuaucug caggagauuu ucuccgaaga gaugggaaag      420

guugacgauu cuuucuuuca ccgccucgaa gacagcuucc uggugacaga ggauaagaga      480

ggcgagcggc auccuaucuu uggcaaucug gaagaggaag ucaaauacca cgagaacuuc      540

cccaccaucu aucaucuccg ccaauaccug gccgacaauc cugagaaggu ggaucuuaga      600

cugguuuauc ucgcucuggc ccacauuauc aaauuucggg ggcacuuccu uauugaagga      660

aaguuugaca cacgcaacaa ugaugugcag agacuguucc aggaguuucu cgcggucuac      720

gacaacaccu ucgaaaauuc cucucugcag gagcaaaacg uccaggugga agagauccug      780

acggacaaaa uuagcaaguc cgccaaaaag gaucgggugc ucaagcuguu ucccaaugag      840

aaaagcaacg gcagauucgc ugaauuucuu aagcugaucg uugggaauca ggccgacuuu      900

aaaaagcauu ucgagcucga agagaaagcu ccucugcagu uuucuaagga uaccuacgaa      960

gaggagcuug aagugcugcu cgcccaaauu ggagacaacu augcggagcu guuccugucc     1020

gccaagaaac ucuacgauag cauccugcuu ucuggcaucc ugacagugac cgacgucggg     1080

acaaaggcuc cgcucuccgc cagcaugauu cagcgcuaua acgaacacca gauggaucug     1140

gcucagcuua aacaauuuau ccggcagaag cugucugaca aauacaauga gguguucucc     1200

gauguuagca aggacggaua ugccggcuac auugauggca agaccaacca ggaagccuuu     1260

uauaaauacc ucaaggggcu gcugaauaaa aucgagggau ccggcuacuu ccucgacaag     1320

auugagagag aagacuuucu gcgcaaacaa cggacguucg auaacggguc uaucccccau     1380

cagauccacc uucaggagau gagagcuauu auccgccggc aggcggaauu uuauccuuuc     1440

cuggccgaca aucaagauag aauugagaag cuccugacau uucgcauccc cuacuaugug     1500

ggaccucugg cucggggcaa gagcgacuuc gccuggcuuu ccagaaaaag cgccgauaag     1560

auuacccccu ggaacuucga cgagaucguc gauaaagaau cuuccgcuga ggccuuuauc     1620

aaucgcauga caaacuacga ccucuaucug ccgaaucaga aggugcugcc uaaacacagc     1680

cuccuguacg aaaaguucac cguuuauaac gagcuuacca aagugaagua caagacagaa     1740

caggggaaaa cggcguuuuu cgaugcuaau augaagcagg agauuuuuga cggagucuuc     1800

aaaguguacc ggaaggucac caaagauaag cugauggacu uucucgagaa ggaguucgau     1860

gaauuuagaa ucguggaccu gacaggccug gacaaagaaa acaagguuuu caaugccucu     1920

uauggcaccu accaugaucu uugcaaaauu cucgacaagg auuuucugga caacagcaaa     1980

aaugagaaga uccuggaaga uauugugcuc acacugaccc uuuucgagga ccgggagaug     2040

auccgcaaga gacuggaaaa cuauuccgau cuccugacga aagagcaagu caagaaacug     2100

gaacggagac acuacacagg guggggacgc cuuucugccg agcucauuca uggcauccgg     2160

aacaaggaaa gcagaaaaac cauccuggac uaucugauug augacggaaa uuccaaccgc     2220

aauuuuaugc agcucaucaa cgaugacgcu cugagcuuca aggaggagau ugccaaggcu     2280

caggugaucg gggaaaccga uaaucuuaac cagguugugu cugacauugc cggcuccccc     2340

gcgaucaaaa aggggauccu gcaaagccuc aaaauugucg acgagcuggu gaagaucaug     2400

ggccaccagc cugaaaauau ugugguugag auggccagag agaaccaguu cacaaaucag     2460

ggacggcgca acucucaaca gagacugaaa ggccuuaccg auuccaucaa ggaauuuggg     2520

agccagauuc ucaaagagca ucccgucgaa aauucccaac ugcagaacga ccggcuguuc     2580

cucuacuauc ugcagaaugg acgcgauaug uacacaggcg aggaacuuga caucgauuac     2640

cugucucagu augacaucga ucacauuauc ccccaggcuu uuauuaagga caacagcauc     2700

gauaauagag ugcucacguc cucuaaggag aaccggggga aaagcgacga ugugccuucc     2760

aaggacgucg ugcgcaaaau gaaaagcuac uggucuaaac ugcuguccgc caaacuuauu     2820

acccaaagaa aguucgacaa ccugacaaaa gcugagcggg gaggccucac cgaugacgau     2880

aaggccgggu uuaucaaacg ccagcugguu gaaacaagac agaucaccaa gcacguggcg     2940

cggauucucg acgagagauu caauacggaa accgaugaga acaauaaaaa gauccgccaa     3000

gucaagauug ugacacugaa aagcaaccuu guuucuaauu uucggaagga guucgaacug     3060

uauaaaguga gagagaucaa cgacuaccau cacgcccaug augcuuaucu caaugccguc     3120

auuggcaagg cucugcuggg aguguacccg cagcuugaac ccgaguuugu uuauggcgac     3180

uacccucacu uccacgggca uaaagaaaac aaggccaccg ccaagaaauu uuucuacucc     3240

aauaucauga acuucuuuaa gaaagaugac gugcgcacag auaagaaugg agagauuauc     3300

uggaaaaagg acgaacacau cagcaacauu aagaaagucc ugucuuaucc ccaggugaau     3360

aucgugaaga aagucgagga gcagaccggc ggguucagca aggaauccau ucucccuaaa     3420

ggaaacagcg auaagcugau cccccggaaa acgaagaagu uuuacuggga cacaaagaag     3480

uauggcgggu ucgacucucc gauuguugcu uacuccaucc ucgugaucgc ggauauugag     3540

aaaggcaaga gcaaaaagcu gaagaccgug aaagcccuug ucggagugac aaucauggaa     3600

aagaugaccu uugagagaga cccuguugcu uuccuggagc gcaaaggcua ucggaaugug     3660

caagaagaga acauuaucaa gcuccccaaa uacucccugu uuaagcugga aaacgggaga     3720

aagcgccuuc uggccucugc ccgggagcuc cagaaaggaa augaaauugu ccugccuaac     3780

caucucggca cccugcuuua ucacgcuaag aauauccaua aaguggauga gcccaagcac     3840

cuggacuacg uugauaaaca caaggacgag uucaaggaac ugcucgaugu ggucagcaac     3900

uuuuccaaaa aguacacacu ggccgagggg aaucuugaaa agaucaagga gcuguaugcg     3960

cagaacaaug gagaagaccu caaagagcug gcuucuagcu ucauuaaccu ccugacguuu     4020

accgccaucg gcgccccugc uacauucaag uuuuucgaua aaaauauuga cagaaagcgg     4080

uacaccucca caaccgagau ccuuaacgcc acgcugauuc aucaaucuau cacagggcug     4140

uaugaaaccc gcauugaucu caauaagcug ggcggagaca aaagacccgc ggcuaccaag     4200

aaagccggcc aggccaagaa aaagaaguaa ggacuagucc cuguucccag agcccacuuu     4260

uuuuucuuuu uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                4370


<210>  4707
<211>  3503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4707
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcccag acaacaaacc ugagcuacau ucucggacug      180

gaccuuggca ucgcguccgu ugggugggcu gugguggaga uuaaugaaaa cgaggauccg      240

aucggacuga uugacgucgg cgugcggauc uucgaacgcg ccgagguucc caagacgggg      300

gaaucucucg cccugagcag acggcuugcu cgcuccacca gacggcugau ccgcagacgg      360

gcccaucgcc uccugcuggc uaaaagauuu cucaagcggg aggggauucu gucuacaauc      420

gaucuugaga agggccugcc uaaucaagcc ugggaacucc gcguggcggg ccuggagaga      480

cggcuuagcg ccauugaaug gggagcuguc cugcuccacc ugaucaaaca ucgcggauau      540

cuguccaagc ggaaaaacga gucucagacc aauaacaagg agcucggcgc ccugcuuagc      600

gggguggcuc agaaucacca gcugcuccaa uccgacgauu acagaacacc cgccgaacug      660

gcgcuuaaaa aguucgccaa ggaggaagga cauauuagaa accagcgcgg cgcuuauacc      720

cacacauuua aucggcugga ccuccuggcc gagcugaacc uccuguucgc ucagcagcac      780

caauuuggga auccucauug caaagaacac auccagcagu acaugaccga gcuucugaug      840

uggcagaagc ccgcccucag cggagaggcc auucugaaaa ugcuuggcaa guguacgcau      900

gaaaagaacg aguucaaagc ugcgaaacac accuauucug ccgaaagauu uguuuggcug      960

acaaagcuca auaaccugcg cauccuggag gauggcgcug aacgggcccu caaugaggag     1020

gaaagacaac ugcuuaucaa ccacccuuac gagaagucca aacugaccua ugcccaggug     1080

cgcaagcucc uggggcugag cgaacaggcu auuuucaaac aucuucggua cucuaaggag     1140

aacgccgaau ccgcgacauu uauggagcuc aaggcuuggc acgccaucag aaaagcccug     1200

gagaaucaag gacugaagga caccuggcag gaucucgcua aaaagccgga ccugcuugau     1260

gaaauuggca cggccuucag ccuguacaaa acagacgagg auauccagca guaucucacc     1320

aacaaggucc ccaauucugu gauuaacgcu cugcuggugu cccuuaauuu ugacaaauuc     1380

aucgaacuca gccugaaguc ccugcgcaag auccucccuc ugauggagca agggaaacgg     1440

uacgaccagg ccugcagaga gauuuaugga caucacuacg gcgaagcgaa ccagaagaca     1500

ucucagcuuc ugcccgccau cccugcucaa gagauucgca aucccgucgu ucuccggacc     1560

cugagccagg ccagaaaagu gaucaacgcu auuauccggc aguauggguc cccggcccgc     1620

gugcauaucg aaaccggaag agagcugggc aagagcuuua aagaacggag agagauucag     1680

aagcaacagg aggauaaucg cacaaagcgg gaaucugcgg uccagaaauu caaggagcuu     1740

uucuccgacu uuagcucuga accuaaaagc aaggauaucc ucaaauucag acuguacgag     1800

cagcaacacg gcaagugucu guauuccggg aaagaaauua acauccaccg ccucaaugag     1860

aagggauacg uggagauuga ccaugcccug cccuuuucua gaacguggga ugacagcuuc     1920

aacaauaagg uucuugugcu ggcuuccgaa aaccagaaua aaggcaacca gaccccuuac     1980

gaguggcucc aagggaagau caacagcgaa cgguggaaaa auuuugucgc ccuggugcug     2040

ggaucucagu gcuccgcugc caagaaacag cgccuucuca cacagguuau ugaugacaac     2100

aaguucaucg auagaaaucu gaacgacacc cgguauaucg cccgcuuucu gagcaauuac     2160

auucaagaga accuccugcu ugugggcaag aauaaaaaga acgucuucac acccaauggg     2220

cagaucaccg cucugcucag aucucggugg ggccugauua aagcgcgcga aaacaauaac     2280

agacaccaug cccuggaugc uaucgugguc gccugugcca cgcccuccau gcagcagaag     2340

auuacacggu uuauccgcuu caaagaggug cacccuuaua agaucgagaa uagauacgaa     2400

augguugacc aagagagcgg agaaauuauc uccccgcacu uucccgagcc uugggcuuau     2460

uuccggcagg aggugaacau uagagucuuu gacaaucauc ccgauaccgu gcuuaaggaa     2520

augcugccug accgccccca ggccaaccac caguucguuc aaccucucuu cgugucucgg     2580

gcgccgacca gaaaaaugag cggccagggg cauauggaga caaucaaguc cgcuaaacgc     2640

cuggccgaag gaauuucugu ccuccggauc ccccugaccc agcuuaagcc uaaccugcuc     2700

gagaauaugg ugaacaaaga aagagagccc gcccuguacg cuggccugaa ggcccgccuu     2760

gcggaguuua aucaagaucc ugcuaaggcc uucgccacac ccuuuuauaa acaaggggga     2820

caacagguga aggcuauccg gguugaacag guccagaaaa gcggcgugcu ggugagagag     2880

aacaaugggg ucgccgacaa cgcuuccauu gugcgcacgg auguuuucau caagaauaac     2940

aaauuuuucc ucgugccgau uuacaccugg caggucgcca agggcauccu gccuaauaaa     3000

gcgauugugg cccacaagaa cgaagacgag ugggaagaga uggaugaggg agcuaaguuu     3060

aaauucagcc ucuuucccaa ugaccugguu gaacuuaaga ccaaaaagga guacuucuuu     3120

ggcuauuaca ucgggcugga ucgggccaca ggaaacaucu cucucaaaga acaugacggc     3180

gagauuucca aggggaagga uggaguguau agagucggcg ugaaacuggc ucugagcuuc     3240

gaaaaguacc agguugacga gcuugggaaa aaucggcaga ucugccgccc ccagcagaga     3300

cagccugugc ggaagcgccc ggccgccacc aaaaaggcug gccaagcgaa gaaaaagaaa     3360

uaaggacuag ucccuguucc cagagcccac uuuuuuuucu uuuuuugaaa uaaaauagcc     3420

ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaga auu                                             3503


<210>  4708
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4708
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccgcg gcuuucaagc cgaaccccau uaauuacauc      180

cugggacucg acauuggcau cgccagcguu gggugggcca ugguggagau ugaugaagac      240

gagaacccua ucugccugau cgaucuugga gugcgggucu uugaacgcgc ugaggugccc      300

aaaaccggcg acucccuggc cauggcuaga cggcucgccc gcucuguuag acggcugaca      360

cgcagacggg cgcaucgccu ucugagagcc cggcgccucc ugaagagaga aggggugcug      420

caggcugccg auuucgacga gaauggacuc auuaagagcc ugccuaacac gcccuggcaa      480

cuucgggcug ccgcgcugga ucgcaaacuc accccucugg agugguccgc cguccuucug      540

caccucauca agcauagagg cuaucugucu cagcggaaaa augaaggcga gacagcugac      600

aaggaacugg gggcccuccu gaaaggagug gcugauaacg cccacgcccu ucagaccggc      660

gacuuuagaa caccggcuga gcuggcgcuc aauaaguucg agaaggaaag cgggcauauu      720

cgcaaccagc ggggagauua cucccacacc uuuucuagaa aagaccugca agccgagcuu      780

auccuccugu ucgaaaagca gaaagaguuu ggcaaucccc acguuagcgg gggacugaag      840

gaaggcauug agacacugcu caugacccag cgcccugcuc uguccggcga ugccgugcag      900

aaaaugcuug ggcauuguac guucgagccc gccgaaccua aggcugccaa aaacaccuau      960

acagcggagc gguuuaucug gcugaccaag cucaauaacc ugcgcauucu ugaacaagga     1020

agcgagagac cccugacaga caccgaacgg gcuacgcuca uggacgagcc guacagaaag     1080

ucuaaacuga cauaugccca ggcccgcaag cugcucggcc uggaggauac cgcuuucuuu     1140

aaagggcuuc gguacggaaa ggacaaugcc gaggcuucca cacugaugga aaugaaagcc     1200

uaucacgcga ucagcagagc ccucgaaaag gagggccuga aggauaaaaa gucuccucug     1260

aaccuuuccc ccgaacucca ggacgagauc gggaccgcuu ucagccuguu uaaaaccgau     1320

gaggacauua caggacgccu gaaggaucgg auccagccug aaauucucga ggcccugcuu     1380

aaacauaucu cuuucgacaa guucguccaa auuucccuga aggcucucag acggaucgug     1440

ccccugaugg aacagggcaa acgcuacgau gaggccugcg cggagaucua cggcgaccac     1500

uaugggaaga aaaauacgga agagaagauu uaccugcccc cuaucccggc cgaugaaauu     1560

agaaaccccg ugguccuucg ggcucucagc caggccagaa aaguuaucaa cggaguggug     1620

cgccgguaug gcuccccugc ucgcauucac aucgagaccg ccagagaagu cgggaagucu     1680

uuuaaggaca gaaaagagau cgagaagcgg caagaagaga aucgcaaaga cagagaaaag     1740

gccgcugcga aauuccggga guacuuuccc aacuucgugg gagaaccuaa gagcaaagau     1800

auucugaagc ugcgccucua ugagcagcag cauggcaagu gucuguacuc cgggaaagag     1860

aucaaucuug gcagacugaa cgaaaaggga uauguugaga uugaccacgc ccuccccuuu     1920

agccggacau gggaugacuc uuucaauaac aaagugcugg uccugggcuc cgaaaaucag     1980

aacaagggga aucaaacccc guacgaguac uuuaacggaa aagauaauag ccgcgaaugg     2040

caggaguuca aggcuagagu ggagacaucu cgguuuccuc gcagcaagaa acagagaauc     2100

cuucuccaga aguucgacga agauggcuuu aaagagcgga accugaauga caccagauau     2160

guuaaccgcu uccugugcca auuuguggcc gaucggauga gacucacggg gaagggcaaa     2220

aagcgcgucu ucgccuccaa uggacagauu acaaaccugc uucggggguu cuggggccug     2280

agaaaggugc gcgcugaaaa cgaccggcau cacgcccucg augcggucgu gguugcuugu     2340

ucuaccgugg ccaugcagca gaaaaucacc agauuugucc gcuacaagga gaugaaugcc     2400

uucgacggaa aaacaauuga uaaggagacc ggcgaagugc ugcaucagaa aacacacuuu     2460

ccccagccuu gggaguucuu ugcucaggaa guuaugaucc ggguguucgg gaagcccgac     2520

ggaaagccug aguuugaaga ggccgacacg cccgagaaac ugagaacccu ucucgcggaa     2580

aagcugagcu cccggccgga ggcuguccac gaauauguga caccucuguu cgugagccgc     2640

gcccccaaca gaaaaauguc uggccagggg cauauggaga ccguuaaguc cgccaaacgg     2700

cucgaugaag gagucagcgu gcugcgcgug ccccuuacac aacugaagcu caaagaccug     2760

gagaagaugg ucaauagaga gcgggaaccu aagcuguacg aggcucuuaa agccagacug     2820

gaagcucaca aggaugaccc ggccaaagcg uuugccgagc ccuucuauaa guacgauaaa     2880

gcuggcaacc gcacccaaca ggugaaggcc guucgggugg aacaagucca gaagacgggg     2940

guguggguua gaaaucauaa cggcaucgcu gacaaugcca ccauggugcg cgucgaugug     3000

uuugagaaag gagacaagua uuaccucguu ccuauuuacu cuuggcaagu ggccaaaggc     3060

auccugcccg aucgggcugu cgugcagggg aaggacgagg aagauuggca gcucauugac     3120

gauuccuuca acuuuaaauu cagccugcac ccuaaugacc uuguggaggu caucacaaag     3180

aaggcgagaa uguucggaua uuuugccucc ugccaccgcg gcaccgggaa cauuaauauc     3240

cggauccaug accuggauca caaaauugga aagaacggca uccucgaagg gauuggcguu     3300

aaaacagcuc ugucuuucca gaaguaccag aucgacgagc ugggaaaaga gauuagaccc     3360

ugucgccuua agaagagacc ucccgugcgg aaacgcccgg ccgccaccaa gaaagcuggc     3420

caggccaaga aaaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3480

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  4709
<211>  3587
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4709
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaag uaccauguug gaauugacgu gggcaccuuc      180

agcguggggc uggcggcuau cgaggucgau gacgccggaa ugccgauuaa aacacucucc      240

cuggugucuc acauccauga uagcggccuu gaccccgaug aaauuaaguc cgccguuacg      300

cggcuggcuu cuagcgggau cgcccgcaga acccggcgcc ucuauagacg gaagcgcaga      360

cggcugcagc aacuugacaa auuuauccag cgccagggau ggccugugau ugagcuggaa      420

gauuacuccg acccccucua uccuuggaag gucagagcug agcuggccgc gucuuacauc      480

gccgaugaaa aagagcgggg cgagaagcug agcguggcuc uccgccacau ugccagacau      540

cggggcuggc gcaaccccua ugcuaaaguu uccagccugu accuuccuga cgggccgucu      600

gaugccuuca aggcgaucag agaagagauu aagcgggccu ccggacagcc cgugccugaa      660

acagcuaccg ucggccaaau ggugacacug ugcgagcucg ggacccugaa acuuagagga      720

gagggcgggg ugcugagcgc ccgccuccag cagucugacu augcucggga aauccaggag      780

auuuguagaa ugcaagaaau cggacaggag cuguaccgca agaucauuga ugucguuuuu      840

gccgccgaau cccccaaagg cagcgcuucu ucccgggugg gcaaggaccc ucugcagccc      900

gggaaaaaua gagcgcucaa ggccagcgac gcuuuccagc gcuaccggau cgccgcccug      960

auuggaaacc uuagagugcg cgucgauggc gagaaacgga uccuguccgu ggaggaaaag     1020

aaucucguuu uugaccaccu ggugaaccuu acaccgaaga aagagccuga augggucacc     1080

auugcugaga uccuggggau cgauagagga caacucauug gcacggccac caugacagac     1140

gauggggaac gcgcgggagc ucggcccccu acccacgaca caaauagauc uaucgugaac     1200

agccggauug cuccccuggu ugauuggugg aagaccgccu ccgcccugga gcagcaugcc     1260

auggugaaag cucucagcaa ugccgagguc gacgauuucg acucucccga aggcgcgaag     1320

gugcaggccu uuuucgcuga ucuggacgac gauguccacg ccaaacuuga cucccugcau     1380

cucccugugg gccgcgcugc cuauagcgag gauacgcugg uuagacuuac acggagaaug     1440

cugucugacg ggguggaucu cuacaccgcg cgccugcaag aauuuggaau cgagccgagc     1500

uggacacccc cuacaccccg gauuggcgaa ccugucggga accccgccgu ggacagaguu     1560

cugaagaccg ugucccgcug gcucgagucu gcuaccaaga cguggggagc cccggagaga     1620

gucaucaucg aacacgugcg ggagggcuuc gugaccgaaa aacgcgcuag agagauggau     1680

ggggacaugc ggcgcagagc cgcccggaau gcuaagcugu uucaggagau gcaggaaaaa     1740

cuuaacguuc agggcaagcc uagccgcgcg gaucugugga gauaucaauc cguccagcgg     1800

cagaauugcc agugugccua cugcggaagc cccauuacau ucucuaacuc cgagauggac     1860

cacaucgugc cucgcgcugg ccaagggagc accaauacaa gagaaaaccu cguggccguc     1920

ugucaucggu gcaaucaguc uaaaggaaac acccccuuug ccauuugggc uaagaacacg     1980

uccaucgagg gcgugagcgu uaaggaagcc guggagagaa cacgccacug ggucaccgau     2040

accgggaugc gguccacaga cuucaaaaag uucaccaaag cggugguuga gagauuucag     2100

cgcgcuacaa uggaugaaga gauugacgcc cggucuaugg aaagcguggc cuggauggcu     2160

aaugagcuga gaucccgcgu cgcccagcau uucgcgucuc acggaacgac cgugcggguu     2220

uauagaggca gccugacagc ugaagcccgc cgggccuccg ggaucagcgg caagcugaaa     2280

uuuuucgacg gagugggcaa gucuagacuc gaucggcgcc aucacgcuau ugacgccgcu     2340

gucaucgccu uuaccuccga uuacguggcg gagacacugg ccgugagaag caaccugaag     2400

caaucucagg cucaccggca ggaggccccu caguggcgcg aauuuaccgg gaaagacgcu     2460

gagcauagag ccgccuggcg ggucuggugu caaaagaugg aaaaacucuc cgcucugcuu     2520

acggaggauc ugagagacga ucgcguugug gugaugagca auguccggcu cagacuggga     2580

aacggcucug cgcacaagga aaccaucggg aaacugagca aggugaaacu uuccagccag     2640

cucucuguuu ccgacauuga uaaggccagc uccgaggcuc uguggugcgc ccugacacgc     2700

gagcccggau uugacccgaa ggaaggccuc ccugccaauc ccgagcggca uaucagagug     2760

aacgggaccc acgucuaugc uggcgauaau auuggacugu uccccguguc ugccggcagc     2820

aucgcgcuuc gcgggggaua cgcugaacug ggcuccucuu uucaccaugc ccggguuuac     2880

aaaauuacaa gcgggaagaa accugccuuc gcuaugcuca gaguguauac caucgaccug     2940

cugccguacc gcaaccagga ucuuuucucc gucgagcuca agccccaaac gaugucuaug     3000

agacaggccg agaaaaagcu gcgggacgcg cuggcuacag gaaaugccga auaucucggc     3060

uggcuggugg uugacgauga gcuugugguc gacaccagca agaucgccac agaucaggug     3120

aaagcugugg aagccgagcu ggggaccauu cgcagauggc gggucgacgg cuucuuuucc     3180

ccuagcaagc uccgccugag accccugcag augucuaaag aaggaaucaa gaaagagucc     3240

gcuccugagc uuagcaagau uaucgaucgg cccggcuggc ucccugccgu uaacaagcug     3300

uucucugacg ggaaugugac cguggucaga cgcgauuccc ugggacgggu gagacucgaa     3360

agcacagcgc accuucccgu uacguggaaa gugcaaaagc gcccggccgc uaccaaaaag     3420

gccggccagg cuaaaaagaa gaaauaagga cuagucccug uucccagagc ccacuuuuuu     3480

uucuuuuuuu gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   3587


<210>  4710
<211>  3287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4710
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccgcg cggauucugg cuuucgacau cggaauuagc      180

uccaucggcu gggccuuuuc ugagaacgau gaacucaagg acugcggggu ucgcauuuuc      240

accaaagugg agaauccgaa gacaggagaa agccuggccc uucccagacg gcuggcucgc      300

uccgccagaa agcggcucgc ucgcagaaaa gcccggcuga accaucuuaa gcaccugauc      360

gcgaaugagu uuaaacucaa cuacgaagau uaucagucuu ucgacgagag ccuggccaag      420

gcuuacaaag gcucccugau cucuccuuau gagcuccgcu uuagagcccu gaaugaacuu      480

cugagcaagc aagauuucgc ucgggugauu cuccauaucg ccaagcgcag aggguacgac      540

gauauuaaaa acuccgacga uaaggagaaa ggagcgaucc ugaaggccau uaaacagaau      600

gaagagaaac uugcuaacua ucagagcguc ggcgaguacc uguauaaaga auacuuucag      660

aaguucaagg aaaauucuaa agaauuuacg aacgugcgga auaagaaaga guccuacgaa      720

cgcuguaucg cccagagcuu ccucaaggac gagcugaaac ugauuuuuaa gaagcaaaga      780

gaguucggcu uuucuuucuc caaaaaguuc gaagaggaag uucucagcgu ggcuuuuuau      840

aaacgggccc ugaaggauuu cucucaccuu gucgggaacu gcuccuuuuu caccgacgag      900

aaaagagccc ccaagaauag cccucuggcu uuuauguucg uggcgcucac acgcaucauc      960

aaccugcuua auaaccugaa gaacaccgaa ggcauucucu acacaaaaga ugaccugaau     1020

gcccugcuca acgagguucu gaagaaugga acccuuacau auaaacagac caagaaacug     1080

cucgggcugu ccgacgauua cgaguuuaag ggagaaaagg gcacguauuu caucgaguuu     1140

aaaaaguaca aagaguucau uaaggcucuu ggggagcaua accugucuca ggacgaucuc     1200

aaugaaaucg ccaaagacau uacccugauc aaggaugaga ucaaacugaa gaaggcccuc     1260

gcuaaauaug accugaacca aaaucagauu gauagccuuu ccaagcugga guuuaaagac     1320

caccucaaca ucagcuucaa ggcccugaaa cuggugacac cccuuaugcu cgaaggaaag     1380

aaguacgaug aggcguguaa ugaacugaac cugaaagucg cuauuaauga ggacaagaaa     1440

gauuuucucc cugccuucaa cgagaccuac uauaaggacg aagugacaaa uccggugguc     1500

cugcgggcca ucaaagagua cagaaagguu cuuaacgcuc ugcucaagaa auauggcaag     1560

gugcacaaaa uuaauaucga acuggcccgc gaggugggca agaaccauuc ucagcgggcu     1620

aaaaucgaaa aggagcagaa cgagaauuac aaggccaaaa aggacgcgga acuggagugc     1680

gaaaaacuug ggcucaagau uaacuccaaa aauauccuga agcugagacu cuucaaagag     1740

caaaaggaau uuugugccua uagcggagag aagauuaaaa ucucugaucu gcaggacgag     1800

aagaugcuug aaauugauca caucuacccc uauagccgcu ccuucgacga uucuuacaug     1860

aacaaagucc ugguguuuac caagcagaau caagagaaac ucaaccagac gccuuucgag     1920

gcuuuuggca augacagcgc caaguggcag aagauugaag uucuggcuaa aaaccugccc     1980

acaaagaaac agaagcggau ccuugauaaa aauuacaagg acaaggaaca aaagaacuuc     2040

aaggauagaa aucucaacga cacccgcuau aucgcccggc uggugcugaa uuacacaaaa     2100

gauuaucucg acuuucugcc ucuuuccgau gacgagaaca ccaagcugaa ugacacccag     2160

aaagggagca agguccaugu ggaggcgaag ucuggaaugc ucacauccgc ccugagacac     2220

acguggggcu ucagcgcuaa agaucgcaac aaccaucugc accacgccau ugacgcuguu     2280

aucauugccu acgccaauaa cucuaucgug aaggcuuuuu ccgauuucaa aaaggaacag     2340

gagagcaauu ccgcggaacu uuaugccaaa aagauuucug agcucgacua caagaacaaa     2400

cggaaguuuu ucgagcccuu uagcggguuc agacagaaag uccuggauaa gaucgacgaa     2460

aucuucgugu ccaaaccgga gcggaagaaa ccuucuggag cucugcauga agagaccuuu     2520

cgcaaggaag aggaguucua ucaaagcuac ggcggcaagg aagggguccu caaagcccug     2580

gagcuuggaa agauuagaaa agugaauggc aagaucguua aaaacgggga uauguuucgg     2640

guggacauuu ucaagcacaa gaaaacaaau aaguuuuacg ccguccccau cuauaccaug     2700

gauuucgcuc ugaaagugcu cccuaacaag gccguugcga gauccaaaaa gggagaaauu     2760

aaggacugga uccugaugga ugagaauuac gaauuuugcu ucagccugua uaaagacucu     2820

cuuauccuga uucagacaaa ggauaugcag gagcccgagu uuguguacua uaacgcuuuc     2880

accuccagca cggucucucu caucgugucc aaacaugaca auaaguuuga aacacugagc     2940

aaaaaccaga agauucucuu caagaaugcc aacgagaaag aagugaucgc caagucuauu     3000

ggcauccaaa aucugaaagu uuucgagaag uacaucguca gcgcucuugg ggaagugacc     3060

aaagccgagu uucgccagcg ggaggacuuc aagaaaaaga gacccgcggc uaccaagaaa     3120

gccggccagg ccaagaaaaa gaaauaagga cuagucccug uucccagagc ccacuuuuuu     3180

uucuuuuuuu gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   3287


<210>  4711
<211>  3698
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4711
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccagc gaccugguuc ucggacugga uauuggcauc      180

ggguccgugg gagugggcau ucuuaacaag gucaccgggg agaucauuca uaaaaauucu      240

cggaucuucc cggcggcuca ggccgaaaac aaucuggugc gcagaacaaa ccggcaagga      300

cgcagacucg cccggcgcaa gaagcacaga cggguucgcc ugaauagacu uuuugaggaa      360

agcggccuga ucacggacuu caccaaaauu uccaucaacc ucaaucccua ccagcugcgg      420

gugaagggcc ugacagauga gcucucuaac gaggagcugu uuauugcucu uaaaaauaug      480

gucaagcauc gcgggaucag cuaucuggac gaugccuccg acgauggaaa cucuagcgug      540

ggcgacuacg cucagauugu uaaagaaaau uccaagcagc ucgaaaccaa gacaccuggg      600

caaauccagc uggagagaua ucagaccuac ggacagcuuc ggggcgauuu cacaguggaa      660

aaagacggga agaaacaccg ccugauuaac gucuuuccca ccagcgccua uagaucugag      720

gcgcuccgga uccugcaaac gcagcaggag uucaauccuc agaucaccga ugaauuuauu      780

aacagauacc uggagauccu cacaggaaag cgcaaauauu accauggccc cggcaaugaa      840

aagucccgga ccgacuacgg gagauaucgc acaagcggag agacccugga uaacauuuuc      900

ggcauccuua uugggaaaug cacguuuuac ccugacgagu uccgggccgc uaaggccucu      960

uauacagcuc aagaauuuaa ccugcucaau gaccugaaca aucuuaccgu gccgacagag     1020

accaagaaac uguccaagga acagaaaaac cagaucauca auuacgugaa gaacgagaaa     1080

gccaugggac ccgcgaaacu cuucaaguau auugccaaac ugcugagcug ugaugucgcu     1140

gacaucaagg gcuacagaau ugauaaaucu gggaaggccg aaauccacac cuuugaggcu     1200

uaucgcaaaa ugaagacacu cgaaacgcug gacauugagc aaauggaucg ggagacccuu     1260

gacaagcugg ccuacguucu cacacugaau accgaaagag agggaaucca ggaagcccuu     1320

gagcacgagu ucgcugaugg cuccuucagc cagaaacagg uggacgaacu ggugcaauuu     1380

cgcaaggcga acuccucuau cuucggcaaa ggguggcaua auuuuagcgu caagcucaug     1440

auggagcuga uuccugaacu guacgagaca uccgaggaac agaugaccau ccucacgcgg     1500

cugggaaaac agaagacaac cucuucuucc aacaagacca aauauauuga ugagaaacuu     1560

cugacagaag agaucuacaa ucccgugguu gccaagagcg ugagacaggc uauuaagauc     1620

gucaacgccg ccauuaaaga auauggcgac uucgauaaua ucgugaucga gauggcucgc     1680

gaaaccaacg aggacgacga gaagaaagcc auucaaaaga uccagaaggc gaauaaagau     1740

gaaaaggacg cugccaugcu caaagccgcu aaccaguaca acgggaaggc cgagcugccu     1800

cacucuguuu uucauggaca caaacagcug gcuacaaaga uucggcuuug gcaccaacag     1860

ggcgaaagau gccucuauac ggggaagacc aucagcauuc augaucugau caauaacucc     1920

aaucaguucg agguggacca cauccugccc cucucuauua cauuugauga cagccuggcc     1980

aacaaagucc uuguguacgc gaccgccaau caggaaaagg gccaacggac accguaucag     2040

gcucuggauu ccauggacga ugccuggagc uuccgcgagc ucaaggcuuu ugucagagag     2100

ucuaagaccc uguccaacaa aaagaaggaa uaccugcuua cggaggaaga caucagcaaa     2160

uucgaugugc ggaagaaauu uauugagaga aaucucguug acacccgcua cgccucucgg     2220

gugguccuga acgcgcugca ggaacauuuc agagcccaca agaucgauac aaaagugucc     2280

guugugcgcg gacaauuuac cagccagcuc agacggcauu ggggcauuga gaagacacgc     2340

gacaccuauc accaccaugc ugucgacgcc cugaucaucg cugccuccuc ucagcuuaau     2400

cuguggaaga aacagaagaa cacgcucgug agcuacuccg aggaucaacu gcuggacauu     2460

gaaacagggg agcuuaucuc ugaugacgaa uauaaagaga gcguguucaa ggccccuuac     2520

cagcacuucg uugauacccu caaauccaag gaguuugaag acagcauucu guucucuuau     2580

caggucgauu ccaaauuuaa uagaaagauc agcgacgcua caauuuacgc gacccggcag     2640

gccaaggugg gaaaagauaa ggcugacgag accuaugugc ugggcaaaau caaggauauc     2700

uacacacaag acggguacga ugccuucaug aaaauuuaua agaaggacaa aucuaaguuu     2760

cucauguacc gccaugaccc ccagacguuc gaaaaaguca ucgagccuau ucuggaaaac     2820

uaucccaaua agcagaucaa cgagaaagga aaggaggugc ccuguaaucc uuuucuuaag     2880

uacaaagagg aacacggcua uauuagaaag uacuccaaaa aggggaacgg cccggaaauc     2940

aaaagccuga aguauuacga uucuaagcuc ggaaaccaca uugacaucac ccccaaagau     3000

agcaauaaca agguugugcu gcaguccguc agcccuuggc gggccgacgu guacuucaau     3060

aaaacaaccg gcaaguauga gauccugggg cuuaaauacg cugaucucca auuugaaaag     3120

ggaacaggca ccuauaagau uucucaggag aaauacaacg acaucaagaa aaaggagggg     3180

guugauuccg acagcgaguu caaauuuacg cuguauaaga augaucugcu ccuggugaaa     3240

gacacagaaa ccaaggaaca gcaacuuuuc cgcuuucugu ccagaaccau gcccaagcag     3300

aaacauuacg ucgagcucaa gccuuaugau aaacagaagu ucgaaggagg cgaggcccug     3360

auuaaagugc uggggaacgu ugcgaauagc ggccagugca agaagggacu uggcaaaagc     3420

aacaucucca uuuacaaggu gcggacagac guccugggga aucaacacau caucaaaaac     3480

gagggagaca agcccaaacu cgauuucaag cgcccggcug ccaccaagaa agccggccag     3540

gcuaagaaaa agaaauaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu     3600

ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                             3698


<210>  4712
<211>  4256
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4712
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccacc caguucgagg gauuuacaaa ccuguaccaa      180

guuagcaaga cgcuccgguu cgaacugauu ccgcagggca aaacccuuaa gcauauccag      240

gagcaggggu uuauugaaga ggacaaggcg cgcaaugauc acuauaaaga acugaagccc      300

aucauugaca gaaucuacaa aacauaugcu gaucaaugcc uccagcuggu gcagcuugac      360

ugggagaacc uguccgccgc caucgauucu uaccggaagg agaaaaccga agagacacgc      420

aaugcucuca uugaagaaca ggccaccuau agaaacgcua uccaugacua cuucauugga      480

cggacagaua aucugaccga cgccaucaac aagcgccacg cggagauuua uaagggccug      540

uuuaaagccg aacucuucaa ugggaaggug cugaaacaac uuggaacggu caccacaacc      600

gagcaugaaa acgcucugcu cagauccuuu gauaaguuca caaccuacuu uagcggcuuc      660

uacgagaauc ggaaaaacgu guuuucugcc gaagacauca gcacggcuau uccucaccgc      720

aucguucagg auaauuuccc caaguuuaaa gagaacuguc acaucuucac aagacugauu      780

accgccgugc cuucccuucg ggagcauuuc gaaaauguca agaaggcgau cggcauuuuu      840

gugucuacaa gcaucgagga aguuuucucc uuucccuucu auaaccagcu gcucacccag      900

acccaaauug accuguacaa ucagcugcuc gggggaauca gccgcgaggc cggcacagag      960

aaaaucaagg ggcugaacga ggugcuuaac cuggcuauuc agaaaaauga ugagacggcc     1020

cacaucauug cuucucuccc ucauagauuu aucccgcugu ucaagcaaau ucuuuccgac     1080

cggaacaccc ugagcuuuau ccucgaagag uucaaaucug acgaagaggu cauccagucc     1140

uuuugcaagu auaagacacu gcugcgcaau gaaaacgugc ucgagaccgc cgaggcccug     1200

uucaaugaac uuaacagcau ugaucugaca cacaucuuua uuucucacaa aaagcucgag     1260

accaucucca gcgcucugug ugaccauugg gauacgcuua gaaaugcgcu guacgaacgg     1320

agaauuuccg agcucacagg aaaaaucacc aagucugcca aagagaaggu gcagcgcagc     1380

cugaagcacg aagacauuaa ccugcaggag aucaucuccg cugccggcaa agaacucagc     1440

gaggccuuca agcagaaaac cucugaaauu cugucccaug cucacgccgc gcuugaucaa     1500

ccccugccua caacccucaa gaaacaggag gagaaggaaa uccuuaagag ccagcuggac     1560

ucucuucucg ggcuguauca ucugcucgau ugguuugcug ucgacgagag caaugaaguu     1620

gaucccgagu ucuccgcccg gcugacagga auuaaacuug aaauggagcc uucucugagc     1680

uucuacaaca aggccagaaa uuaugcuacg aaaaagcccu acuccgugga gaaauuuaag     1740

cucaacuucc aaaugccgac ccuggccagc ggcugggacg ugaauaaaga aaagaacaau     1800

ggcgcuaucc uguuugucaa gaacgggcuu uacuaucucg gaauuaugcc uaaacagaag     1860

ggccgcuaca aagcccuguc uuucgagccc acagaaaaga ccuccgaggg guuugauaaa     1920

auguauuacg acuauuuccc ugacgcggcc aagaugaucc ccaagugcag cacacagcug     1980

aaagcuguga ccgcccacuu ucagacgcac accacaccca uccuccuguc uaacaauuuc     2040

auugaaccuc uugagaucac caaggagauu uacgaucuga acaauccgga aaaagagccc     2100

aagaaauuuc aaacagcuua ugccaagaag accggagacc agaaaggcua ccgggaagcg     2160

cucuguaagu ggaucgauuu cacgagagac uuucugucca aauacacaaa gaccacaagc     2220

auugaucugu ccucucuucg cccuagcucc caguauaaag accucgggga guacuaugcc     2280

gagcugaacc cccugcucua ccauaucucu uuccagcgga ucgcugaaaa ggagauuaug     2340

gaugccguug aaaccggaaa gcuguaucuu uuucaaaucu acaauaaaga cuucgcuaag     2400

ggccaccaug gcaaaccuaa ccugcacacc cucuauugga cagggcuguu cagccccgag     2460

aaucuggcca agacguccau uaaacuuaac ggacaggccg aacucuuuua cagaccuaag     2520

agccgcauga aacggauggc ucacagacug ggcgagaaga ugcugaauaa gaaacucaag     2580

gaucagaaaa ccccgauccc cgacacacug uaccaagagc uuuaugauua cgugaaccau     2640

cgccugucuc acgaccucuc cgaugaagcg cgggcccugc ugccuaaugu cauuaccaag     2700

gaggugagcc augaaaucau caaagacaga cgguucacau cugacaaguu uuucuuucac     2760

guucccauua cccuuaacua ucaggcugcc aauuccccua gcaaguucaa ccagcgcgug     2820

aaugccuacc ugaaagagca ucccgaaacg ccgaucauug ggaucgauag aggagagcgg     2880

aaccucauuu auaucacagu cauugacucu accggcaaga uccuggagca gagaagccuc     2940

aacaccaucc aacaguuuga uuaccagaaa aagcuggaca aucgcgaaaa agagcgggug     3000

gcugccagac aggcgugguc cgucgugggg acaauuaagg aucuuaagca aggcuaucug     3060

agccagguua uccacgaaau uguggaccuc augauccacu accaggcugu cgugguucug     3120

gagaaccuga auuucggauu uaaaucuaag cgcaccggca uugccgaaaa agccguguac     3180

cagcaauucg agaagaugcu uaucgauaaa cugaacugcc ucguccugaa ggacuauccu     3240

gcugagaagg uugggggagu gcucaauccc uaccagcuga cagaucaguu uacguccuuc     3300

gccaaaaugg gcacccaaag cggguuccug uuuuaugugc ccgcgccuua cacauccaag     3360

aucgacccgc ugaccggauu cgucgauccc uuugugugga aaacaauuaa gaaccaugaa     3420

ucuagaaaac acuuccucga gggcuuugac uuccugcauu augaugugaa gaccggggac     3480

uuuauccugc acuucaagau gaaucggaac cuuagcuuuc agcgcggccu gccuggauuc     3540

augcccgcuu gggacauugu cuuugagaaa aaugaaacgc aguucgaugc caagggcacc     3600

ccuuuuaucg ccgggaaaag aauugugccc guuaucgaga accaucgguu cacaggacgc     3660

uacagagacc ucuauccugc uaaugaacug aucgcccucc uggaggaaaa gggcauugug     3720

uuccgggaug gguccaacau ccuucccaaa cugcuggaga augacgauuc ucacgcuauu     3780

gacaccaugg ucgcccucau ccgcagcgug cugcagauga gaaacuccaa ugcggccaca     3840

ggagaagauu acauuaacuc uccgguucgg gaccuuaacg gcguguguuu ugauagccgc     3900

uuccaaaauc cugaguggcc cauggacgcu gaugccaacg gggcuuacca caucgcccug     3960

aagggccagc uccugcucaa ucaucugaaa gaguccaagg accuuaagcu gcagaacgga     4020

auuagcaauc aggacuggcu ggccuauauc caagaacuca gaaacaaacg gccugcugcg     4080

accaagaaag ccggccaggc uaagaaaaag aaguaaggac uagucccugu ucccagagcc     4140

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         4256


<210>  4713
<211>  4235
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4713
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccagc auuuaccagg aguucguuaa caaguauucc      180

cugucuaaaa cccuccgguu ugaacugauc ccgcaaggaa agacacuuga gaauauuaag      240

gcgcgcggcc ugauccucga cgaugaaaag agagcuaagg acuacaaaaa ggccaaacag      300

auuaucgaua aguaucauca guucuuuauc gaggaaauuc ugagcuccgu gugcaucucu      360

gaggaccuuc ugcagaacua cagcgaugug uauuucaagc ucaaaaaguc cgacgaugac      420

aaucugcaaa aagauuuuaa gucugccaaa gacacgauua agaaacagau cagcgaguac      480

auuaaggauu ccgaaaaguu caaaaaccug uuuaaucaga accucaucga cgcuaagaaa      540

gggcaggaga gcgaucugau ucuuuggcug aagcaaucua aagacaaugg aaucgaacuc      600

uucaaggcca acuccgacau caccgauauu gacgaggcuc uggagaucau uaagagcuuu      660

aaaggcugga caaccuauuu caagggguuu cacgaaaauc ggaaaaacgu cuacucuucc      720

aaugauaucc ccacaagcau uaucuaccgc aucguggacg auaaccuucc uaaguuccug      780

gagaauaaag ccaaguauga aucucucaag gacaaagcgc ccgaggccau uaacuacgaa      840

cagaucaaga aagaucuggc ugaggagcug accuuugaca uugauuauaa gacauccgaa      900

guuaaucaga gaguguucag ccucgacgag gucuucgaaa ucgccaacuu uaacaauuac      960

cugaaccagu ccggaauuac caaauucaau acgaucaucg gcggcaaguu ugugaacggg     1020

gagaauacca agcggaaagg aauuaacgaa uauaucaauc uuuacucuca acagauuaac     1080

gauaagacac ugaaaaagua uaaaaugagc guucucuuca agcagauccu guccgacacc     1140

gagagcaaau cuuuugugau ugacaaacuu gaggaugacu ccgaugucgu gacaaccaug     1200

cagagcuucu acgaacaaau cgcugccuuu aagacggugg aggaaaaauc uauuaaggag     1260

acacugagcc uccuguucga cgaucugaaa gcgcagaagc ucgaccuguc caaaaucuac     1320

uuuaagaaug auaagucucu uaccgaccug agccagcaag ucuucgauga cuauuccguu     1380

aucggcacag ccgugcucga auacauuacc cagcagaucg cuccuaaaaa ccuggauaau     1440

cccagcaaga aagagcagga gcuuauugcc aagaaaaccg aaaaggcuaa guaucugucu     1500

cucgagacaa ucaaacuggc ccuggaagag uuuaacaagc aucgcgacau ugauaaacaa     1560

uguagauucg aggaaauccu cgccaauuuu gcugcgaucc cuaugauuuu cgacgagauc     1620

gcccagaaca aggacaaucu ggcucagauu uccaucaaau accagaacca agggaagaag     1680

gaucuucugc aggccagcgc cgaagacgau gugaaagcua uuaaggaccu ccuggaucag     1740

acgaacaauc ugcuucacaa acucaagauc uuccauaucu cucaguccga ggacaaagcc     1800

aacauucugg auaaggacga acacuuuuau cuggucuucg aggagugcua cuuugaacuc     1860

gcgaauaucg ugccgcugua uaacaaaauu cggaauuaca ucacccaaaa gcccuacagc     1920

gaugagaagu ucaaacuuaa cuuugaaaau uccacacugg cuaacggaug ggacaagaau     1980

aaagagccug auaacaccgc cauucucuuc aucaaggacg auaaauauua ccugggcguu     2040

augaauaaga agaacaauaa aaucuuugac gacaaggcca uuaaagaaaa caagggggag     2100

ggauauaaaa agaucgugua caagcugcuu cccggcgcua auaaaaugcu cccuaagguc     2160

uucuuuucug ccaaaagcau uaaguucuau aaccccuccg aggauauccu gcgcauuaga     2220

aaccacucua cacauaccaa aaauggcagc ccgcagaagg gguacgaaaa guuugaguuc     2280

aacaucgaag acugucggaa auuuauugau uucuauaagc aguccaucag caaacacccu     2340

gaguggaagg acuucggauu ucgcuucucu gauacgcaaa gauacaauuc caucgacgaa     2400

uuuuaccggg agguggagaa ccagggcuau aaacugacau ucgaaaauau uagcgagucu     2460

uacaucgauu ccguugugaa ccaggggaag cucuaucugu uucagauuua caauaaggac     2520

uucagcgcuu auucuaaagg acgccccaac cuucauaccc uguacuggaa ggcccucuuu     2580

gaugaaagaa aucugcaaga cgucguguau aaacugaacg gcgaggcgga gcuuuucuac     2640

cggaagcaga gcaucccuaa aaagauuacc caccccgcca aagaagcuau cgccaauaag     2700

aacaaggaua aucccaaaaa ggaguccguc uuugaauacg accucaucaa agacaagaga     2760

uucacagagg auaaauuuuu cuuccacugc ccuauuacca ucaacuuuaa gagcucuggg     2820

gcuaauaagu ucaacgacga aauuaaccug cugcucaaag agaaggccaa ugaugugcau     2880

auccugucca uugaccgcgg agagcggcac cuugcguauu acacacuggu ugauggcaaa     2940

ggcaacauca ucaagcagga cacguuuaau auuaucggga acgauagaau gaaaaccaau     3000

uaucaugaca agcucgccgc uauugaaaag gaucgcgaca gcgcccggaa agauuggaag     3060

aaaaucaaca auauuaagga gaugaaagaa ggauaccugu cccagguggu ccacgagauc     3120

gcuaagcugg ugauugaaua uaacgccauc guuguguucg aggaccuuaa uuuuggcuuc     3180

aagagagggc gcuuuaaagu cgagaagcaa guguaccaga aacucgaaaa gaugcugauc     3240

gagaaacuga acuaucucgu guucaaggau aaugaauuug acaaaacagg aggcguucug     3300

cgggccuacc agcuuaccgc uccguucgag acauuuaaga agauggggaa acagaccggc     3360

auuaucuacu auguccccgc gggauucacg ucuaagauuu guccugugac cggcuuugug     3420

aaccaacugu accccaaaua ugaaagcguc uccaagucuc aggaguucuu cagcaaauuu     3480

gacaagaucu gcuacaaucu cgauaagggg uauuucgagu uuuccuucga cuacaaaaac     3540

uuuggagaua aggccgcuaa aggcaagugg acaauugccu cuuucgggag cagacugauc     3600

aauuuucgca acuccgacaa aaaucauaac ugggauaccc gggaagugua uccuacaaag     3660

gagcuggaaa aacuucugaa agacuacagc aucgaguacg gacacggcga guguauuaag     3720

gccgcuaucu gcggggaauc ugauaaaaag uucuuugcca aacucaccuc cguucugaac     3780

acgauucucc agaugagaaa uagcaagaca ggcaccgagc uggacuaucu uaucucuccc     3840

guggcggaug ucaacggaaa uuucuuugac ucccgccaag cuccgaagaa caugccucag     3900

gaugccgacg ccaauggcgc uuaccacauu gggcugaaag gacucaugcu gcugggccgg     3960

aucaagaaca aucaggaagg gaaaaagcug aaccuuguga ucaaaaauga ggaauauuuc     4020

gaguuuguuc agaacagaaa uaacaagcgg cccgccgcga caaagaaggc uggacaagcc     4080

aagaaaaaga aauaaggacu agucccuguu cccagagccc acuuuuuuuu cuuuuuuuga     4140

aauaaaauag ccugucuuuc agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                4235


<210>  4714
<211>  4420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4714
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccga caagaaauac agcauuggac uggauaucgg caccaacucc guuggguggg      180

cggugauuac agacgaguau aaggugccgu cuaagaaauu caagguccuc ggaaauacgg      240

aucggcauag caucaaaaag aaccugauug gcgcucugcu guuugacucc ggggaaaccg      300

ccgaggccac acgccucaaa agaaccgcuc ggcgcagaua cacacggcgc aagaauagaa      360

ucugcuaucu gcaggaaauc uucucuaacg agauggccaa gguggaugac agcuuuuucc      420

accggcuuga agaguccuuu cugguugagg aagauaaaaa gcaugagcgc caccccauuu      480

ucggaaauau cguggacgaa gucgcuuacc augagaaaua uccuaccauu uaccaccuca      540

gaaagaaacu gguggauucu acagacaagg ccgaucugcg gcucaucuau cuggcgcuug      600

cccacaugau uaaauuucgc ggccauuucc ugaucgaggg cgaccucaac cccgauaaua      660

gcgacguuga caagcuguuu auucaacuug ugcagaccua caaccagcug uucgaagaga      720

auccuaucaa cgcuuccggg gucgaugcca aggcuauccu cagcgccaga cugucuaaau      780

cccggcgccu ggaaaaucuc auugcgcagc ugcccggaga gaagaaaaac ggccuuuuug      840

ggaaucugau cgcccucagc cugggacuua cgccuaacuu caagucuaau uuugaccugg      900

cugaagaugc caaacuccaa cuguccaagg acaccuacga ugacgaucug gacaaccucc      960

uggcucagau uggcgaucag uaugccgacc uuuuccuggc cgcuaagaac cucagcgaug     1020

cgauccugcu uucugacauu cugagaguga auacagagau caccaaagcc ccgcucuccg     1080

cuagcaugau caagcgguac gacgagcacc aucaggaucu gacacugcuc aaagcccugg     1140

ugagacaaca gcuucccgaa aaguauaaag agauuuucuu ugaccagucc aagaacgggu     1200

acgccggaua uaucgauggc ggcgcuucuc aggaagaguu cuacaaguuu auuaaaccua     1260

uccuggaaaa gauggacggg accgaggagc uccuggucaa acugaaucgc gaagaucuuc     1320

uccggaagca aagaacguuc gacaacggaa gcauucccca ccagauccac cugggcgagc     1380

ugcaugccau ccuccgccgg caggaagauu uuuauccuuu ccugaaagac aauagagaga     1440

agauugaaaa gauccuuaca uuucgcauuc ccuacuacgu ugggccgcug gcgcggggaa     1500

acuccagauu cgcuuggaug acccgcaaaa gcgaggagac aaucaccccu uggaauuuug     1560

aagagguggu ggauaagggc gccucugccc aauccuucau ugaacggaug accaacuuug     1620

acaaaaaucu ccccaacgag aagguccugc cuaaacacag ccugcuuuau gaguacuuca     1680

caguguauaa ugaacucacg aagguuaaau acgugaccga ggggaugaga aagcccgcuu     1740

uucugucugg agaacagaag aaagccaucg ucgaucuucu cuucaagaca aaccgcaaag     1800

ugaccguuaa gcagcugaaa gaggacuauu ucaagaagau ugaauguuuu gauagcgugg     1860

agaucuccgg cgucgaggac cgguucaaug cuucucuugg cacauaccau gaccugcuca     1920

aaaucauuaa ggauaaagac uuucuggaua acgaagagaa ugaagacauc cuggaggaua     1980

uugugcuuac ccucacgcug uucgaagaca gagagaugau cgaggaacgg cugaagacau     2040

augcccaccu cuuugaugac aaagucauga agcagcugaa gcgcagacgg uacaccgggu     2100

ggggaagacu uagccgcaaa cugauuaacg gcauccggga uaagcaaucc gggaaaacca     2160

uccucgacuu ccuuaagagc gauggauuug cgaacagaaa uuucaugcag cugauucaug     2220

acgauucucu gacauuuaaa gaggacaucc agaaggccca gguguccggc caaggggaca     2280

gccuccacga acacauugcu aaccuggccg gcucucccgc uaucaagaaa ggcauucugc     2340

agaccguuaa gguggucgau gagcucguga aaguuauggg acgccauaag ccugaaaaua     2400

ucgugaucga gauggccaga gagaaccaga caacgcagaa agggcaaaag aacucccggg     2460

aacgcaugaa aagaauugag gaaggaauca aggagcuggg cagccagauu cuuaaggagc     2520

acccggucga aaauacccag cugcagaaug agaaacucua ccuguauuac cugcaaaacg     2580

ggcgggacau guauguggau caggaacuug acaucaaucg ccuguccgau uacgacgugg     2640

aucauauugu uccccagucu uuccucaagg acgauagcau cgacaacaaa guccugacaa     2700

gauccgauaa gaaucgggga aaaucugaca acgugccuag cgaggaagug gucaagaaga     2760

ugaaaaauua uuggcgccaa cuccugaacg cgaaacuuau cacccagaga aaauuugaca     2820

aucugacaaa ggccgagcgg ggcgggcucu ccgagcugga uaaagcuggc uucauuaagc     2880

gccagcuggu ggaaaccaga cagaucacga agcacguugc ccaaauucuu gacagccgga     2940

ugaacaccaa auacgaugag aacgacaagc ugaucagaga agugaaaguc auuacacuca     3000

agucuaaacu gguguccgau uuucgcaagg acuuccaguu cuauaagguu cgggagauca     3060

auaacuacca ccaugcucac gaugccuacc ucaaugccgu ggucggaacc gcucugauua     3120

aaaaguaucc caaacuugaa agcgaguuug uguacggcga cuauaagguu uacgauguga     3180

gaaaaaugau cgcgaagucu gagcaggaaa ucgggaaggc cacagcuaaa uauuucuuuu     3240

acuccaacau uaugaauuuc uuuaagaccg agaucacgcu ggccaacgga gaaauucgca     3300

aacggccucu caucgagaca aauggcgaaa ccggggagau ugucugggac aagggaagag     3360

auuucgccac agugcgcaaa gugcugagca ugccccaggu caacaucguu aagaaaaccg     3420

aggugcaaac cggcggguuu ucuaaggaaa gcauccugcc gaagagaaau uccgacaaac     3480

uuauugcucg gaagaaagau ugggacccua agaaauaugg cggauucgac agccccacag     3540

uggccuacuc uguccuggug guugcgaagg uggagaaggg caaauccaag aaacucaaga     3600

gcgucaaaga acugcucggg aucacgauua uggagcgcuc cucuuuugag aagaacccua     3660

ucgauuuccu ggaagcuaag ggauacaaag aggugaagaa agaccuuauu aucaagcugc     3720

ccaaauauag ccuguuugaa cucgagaaug gccggaagag aaugcuggcc uccgccgggg     3780

aacuucagaa gggaaacgag cuggcucucc cuucuaaaua cguuaauuuc cuguaucucg     3840

ccagccauua cgagaagcug aaaggcuccc ccgaagauaa cgagcagaag cagcuuuucg     3900

uggaacaaca caaacauuau cuggacgaga ucauugaaca gaucucugag uuuagcaagc     3960

gggucauucu ggcggaugcu aaucucgaca aagugcuguc cgccuacaac aagcaccgcg     4020

auaagccgau cagagagcag gccgaaaaca uuauccaccu uuucacccug acaaaucucg     4080

gggcuccugc cgcuuuuaaa uauuucgaca ccacaauuga ucggaagcgc uacaccagca     4140

cgaaagaggu ucuggacgcc acacucaucc aucaaucuau caccggccug uacgaaacca     4200

gaauugaucu uucccagcug ggaggcgaca agcggcccgc ggccacaaag aaggcugggc     4260

aggccaagaa aaagaaauaa ggacuagucc cuguucccag agcccacuuu uuuuucuuuu     4320

uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                           4420


<210>  4715
<211>  5203
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4715
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa cuucaagauu cugccgaucg cgauugaccu cggaguuaaa aauacaggcg      180

uguuuagcgc uuucuaccag aaggggacau cccuggagcg gcuugauaac aagaauggaa      240

aaguguauga acugucuaag gacagcuaca cgcuccugau gaacaaucgc accgccagac      300

ggcaucaacg cagaggcauc gaucggaaac agcuugucaa gcgccuguuu aaacucauuu      360

ggacagagca gcugaaccug gaaugggaca aggauaccca gcaagccauc uccuuccucu      420

uuaauagacg gggguucucu uuuaucacag acggauauag ccccgaguac cugaacauug      480

ugccugaaca gguuaaggcu auccuuaugg auauuuucga cgauuauaau ggcgaggacg      540

aucuggacuc cuaccucaaa cuggccaccg agcaggaauc uaagaucagc gagauuuaua      600

acaaacuuau gcagaagauc cuggaauuua aacucaugaa gcugugcaca gauauuaaag      660

acgacaaggu guccaccaag acgcugaaag agaucaccag cuacgaguuc gaacuccugg      720

cugauuaccu ugccaauuau ucugaguccc ugaagacaca aaaauuuagc uacaccgaca      780

agcagggcaa ccucaaagaa cugucuuauu accaccauga uaaguauaau auccaggagu      840

uccuuaagcg ccacgcgaca auuaacgaca gaauccugga uacccuccug acggacgauc      900

uggacauuug gaauuuuaac uucgaaaaau ucgauuuuga caagaacgag gagaaacucc      960

agaaucaaga agauaaggac cauauccagg cccaucugca ccacuucguc uuugcuguga     1020

acaaaauuaa guccgagaug gccagcgggg gacggcaccg cucucaguac uuccaagaaa     1080

ucacaaaugu ucuugacgag aacaaucauc aggaaggcua ucugaagaac uuuugugaga     1140

aucuccacaa caaaaaguac uccaaucuga gcgugaaaaa ccuugucaau cugaucggga     1200

accucuccaa ucuggagcug aagccccuca gaaaauacuu caacgauaag auucacgcua     1260

aggccgacca uugggaugaa cagaaauuua ccgagacaua uugccacugg auccugggag     1320

aauggcgggu gggcgugaag gaccaggaua aaaaggacgg ggcgaaauac ucuuauaagg     1380

aucuuuguaa ugagcugaaa caaaagguca ccaaggccgg acucguugac uuccugcugg     1440

aacuugaucc uugccgcacc auucccccuu accucgacaa caacaauaga aaaccgccca     1500

agugucagag ccugauccug aacccuaaau uucucgauaa ucaguauccc aacuggcagc     1560

aauaccugca ggagcuuaag aaacugcagu ccauucagaa uuaucucgac agcuucgaga     1620

cagaucugaa ggugcugaag ucuuccaaag accaaccuua cuuuguggaa uacaagagcu     1680

cuaaccagca gaucgcuagc ggccagcggg acuauaaaga ucuugacgcc agaauccucc     1740

aauucauuuu ugaucgcguc aaggcuuccg acgagcugcu gcucaaugaa aucuacuucc     1800

aggccaaaaa gcugaagcag aaagccucua gcgagcuuga gaagcuggaa uccagcaaaa     1860

agcucgauga ggugauugcu aacucucaac ugucccagau ccugaaaagc cagcauacga     1920

auggcauuuu cgaacagggg accuuucuuc accucguuug caaguauuac aagcaacggc     1980

agagagcgcg cgacucucgg cuguauauca ugcccgagua cagauaugau aaaaagcugc     2040

auaaauacaa caauacagga cgcuucgacg augacaacca gcuccugacc uacuguaauc     2100

acaagccgcg gcagaaaaga uaucaacuuc ugaacgaucu cgccggcgug cugcaggucu     2160

ccccuaauuu ucugaaggac aaaauuggga gcgaugacga ccuuuucauc uccaaguggc     2220

ucguggaaca cauccgcgga uuuaagaagg cuugcgagga uucucugaaa auucagaaag     2280

acaaccgggg ccugcucaac cauaagauca auauugccag aaacacaaaa gggaagugug     2340

agaaggaaau cuucaaucug auuugcaaaa ucgagggaag cgaagauaag aaaggcaacu     2400

acaagcacgg ccuugccuau gagcuggggg uucuccuguu uggagaaccc aaugaggcuu     2460

ccaaaccuga guucgaccgc aagaucaaga aauuuaacuc uauuuacagc uucgcccaga     2520

uccaacagau ugcguuugcu gaacggaagg gcaaugccaa caccugugcc gugugcuccg     2580

cugauaaugc ccauagaaug cagcaaauca aaauuacgga gcccgucgaa gacaacaagg     2640

auaaaaucau ccugagcgcu aaggcccagc ggcuucccgc gauuccuaca cgcaucgugg     2700

acggggccgu caagaaaaug gcuaccauuc uggccaagaa uaucguggau gacaacuggc     2760

agaauauuaa acagguucuc ucugcuaagc accaacugca caucccgauc auuaccgagu     2820

ccaacgccuu cgaauuugag cccgcgcucg ccgaugugaa aggaaagagc cugaaggaca     2880

gacggaaaaa ggcucuugag agaaucucuc cugaaaauau uuucaaagac aagaacaacc     2940

gcaucaaaga guucgccaag ggcauuuccg cuuauagcgg ggccaaucug acagauggcg     3000

acuuugaugg agccaaagag gaacucgacc auaucauucc ccggucucac aagaaguacg     3060

gcacccugaa cgaugaggcu aaucugaucu gcgucacaag aggggacaac aaaaauaagg     3120

gaaaccgcau cuucugucuu agagaucugg cggacaauua uaaacucaag caguuugaaa     3180

cgaccgauga ccuggagauu gagaaaaaga ucgccgauac aauuugggac gcuaacaaga     3240

aagauuucaa guuuggcaau uaccggagcu ucaucaaccu caccccucag gaacagaaag     3300

ccuuucgcca ugcccuguuc cuugcugacg aaaaucccau uaagcaagcc gugaucagag     3360

cgaucaacaa ucggaaccgc acauuuguua augggaccca gagauacuuc gcugaggugc     3420

uggccaacaa uauuuaucuc cgggccaaaa aggagaaccu gaacacggac aagaucuccu     3480

uugauuacuu cggcauuccg accaucggaa augggcgcgg cauugcugaa aucagacagc     3540

uguaugagaa agucgacagc gauauccagg ccuacgcgaa gggagacaaa ccucaggcuu     3600

cuuauuccca ccuuauugau gccaugcugg ccuucugcau cgcugccgac gaacaucgga     3660

acgauggcag cauugggcuc gagaucgaca agaauuacuc ccuguauccc cucgauaaaa     3720

acacaggaga aguguuuacc aaggacauuu ucucucaaau caagauuaca gauaaugagu     3780

uuagcgacaa aaagcuggug cgcaaaaagg cuaucgaggg cuucaacacc cacagacaga     3840

ugacgcggga ugggaucuac gccgaaaauu accuuccuau ucugauccac aaagagcuga     3900

acgaaguuag aaagggauau acauggaaaa auuccgagga aauuaagauc uuuaagggca     3960

aaaaguacga cauucagcaa cucaacaauc uggucuauug ucuuaaauuc guggacaagc     4020

ccaucucuau cgauauucag aucagcaccc uggaggagcu ccgcaacauu cugacaacca     4080

auaacaucgc ggccaccgcu gaauacuauu acauuaaucu caaaacacag aagcugcaug     4140

aguauuacau cgaaaacuac aacacggccc uuggguauaa gaaauacucc aaggagaugg     4200

aguuucugcg gucucuggcu uauagaagcg aacgcgugaa aaucaagucc auugacgaug     4260

ucaaacaagu gcucgacaag gauagcaauu ucaucauugg caagaucacc cugccuuuua     4320

aaaaggagug gcagcggcuu uacagagaau ggcagaacac aaccauuaaa gacgauuaug     4380

aguuccugaa gucuuucuuc aauguuaaau ccaucacaaa gcuccacaag aaagugcgca     4440

aggacuuuag ccugcccauu ucuaccaacg aaggaaaauu ccucgucaag cggaaaacgu     4500

gggauaauaa cuucaucuac cagauccuga augacuccga uagcagagcc gacggcacaa     4560

agccguuuau uccugccuuc gacaucucua aaaacgagau uguggaggcu aucauugauu     4620

ccuuuaccag caagaauauc uucuggcuuc ccaagaacau cgaacugcaa aaaguugaca     4680

auaagaacau uuuugcgauc gauaccucca aaugguucga gguggaaaca cccagcgacc     4740

ugcgcgauau ugggaucgcc accauucagu auaagaucga caauaacucu cggccuaaag     4800

ucagagugaa gcucgauuac guuaucgacg augacagcaa gauuaauuac uuuaugaacc     4860

auucccugcu uaaaucucgg uauccggaua aggugcugga gauccucaaa cagagcacaa     4920

uuaucgaauu ugaguccucu ggauuuaaca agacgauuaa agagaugcug ggcaugaagc     4980

ucgcugggau cuacaaugaa accagcaaca auaagcgccc cgccgccacc aaaaaggcug     5040

gacaggccaa aaagaaaaag uaaggacuag ucccuguucc cagagcccac uuuuuuuucu     5100

uuuuuugaaa uaaaauagcc ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     5160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                       5203


<210>  4716
<211>  3475
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4716
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gcggaacuac auucugggac ucgacaucgg cauuaccagc guuggguaug      180

gaaucauuga uuacgagaca cgcgacguga ucgaugcggg cgugagacug uucaaagaag      240

cuaaugucga gaacaaugaa gggcggcgcu ccaagagagg agcccggcgc cuuaagagac      300

ggcgcagaca ucggauccag cgcgugaaaa agcugcucuu ugacuauaac cugcuuacgg      360

aucacucuga gcugagcggc auuaauccgu acgaagccag aguuaaaggc cucucccaaa      420

agcugucuga ggaggaguuc agcgcugccc ugcuccaucu ggcuaaacgg cgcggggugc      480

acaacgucaa ugaaguggaa gaggacaccg gaaacgagcu uuccacaaag gaacagaucu      540

cuagaaauag caaggcccug gaggaaaaau auguugcgga gcuccagcug gaacggcuua      600

agaaagaugg cgaggugaga ggguccauua accgcuuuaa gaccagcgac uacgucaaag      660

aggccaagca gcugcucaaa gugcaaaagg cuuaucauca gcuggaucag ucuuucaucg      720

acacauacau ugaucugcuc gaaacccgga gaacauacua ugagggaccc ggcgaagggu      780

ccccuuuugg auggaaggac aucaaagagu gguacgaaau gcugaugggc cacugcaccu      840

auuuccccga ggagcuucgc agcgugaagu acgccuauaa ugcugaucug uacaacgccc      900

ucaaugaccu gaacaaucuu gucauuacgc gggacgaaaa cgagaaacug gaauauuacg      960

agaaguuuca gaucaucgaa aacguuuuca aacaaaagaa gaaaccuacc cucaagcaga     1020

uugcgaaaga gauccuggug aaugaggaag auauuaaggg cuacagagug acaucuaccg     1080

ggaaacccga guuuacaaac cugaaggucu aucacgacau caaggauauu accgcccgca     1140

aagaaaucau cgagaaugcu gagcuccugg accagauugc caagauccuu acgauuuacc     1200

aguccagcga agauauccaa gaggaacuga caaaccucaa uucugagcug acccaggaag     1260

agauugagca gaucuccaac cuuaaaggau auacaggcac ccauaaucug agccucaagg     1320

cuaucaaccu gauucuggac gaacucuggc acaccaauga uaaccaaauc gccauuuuca     1380

aucggcugaa acuugugccu aagaagguug accuguccca gcagaaagag aucccgacaa     1440

cgcucgugga ugacuuuauu cugucucccg ucgugaagcg cagcuucauc caguccauua     1500

aaguuaucaa cgccaucauu aagaaauacg ggcugccuaa ugauaucauu aucgaacuug     1560

cucgcgagaa gaacagcaaa gacgcgcaaa agaugauuaa ugaaaugcag aagcggaaca     1620

gacagaccaa cgagcgcauc gaggaaauca uucggacaac cggaaaagag aaugccaagu     1680

aucucaucga gaaaauuaag cugcaugaca ugcaggaagg caaaugucug uacucucucg     1740

aagcuauccc ccuggaggau cuucugaaca auccuuuuaa cuaugaggug gaccacauua     1800

uccccagauc cgucagcuuc gauaauucuu ucaacaauaa ggugcucguc aagcaagaag     1860

agaacagcaa aaaggggaau cggacaccgu uucaguaccu guccucuagc gacuccaaaa     1920

ucagcuacga aaccuucaag aaacacauuc ugaaccuugc caagggaaag ggccgcaucu     1980

cuaaaacgaa gaaagaguau cuccuggagg aaagagauau uaaucgguuu uccgugcaga     2040

aggacuucau caacagaaau cugguugaua cacgcuacgc cacccggggc cucaugaacc     2100

ugcuuagaag cuauuuucgc gugaauaacc uggacgucaa agugaagucu auuaacgggg     2160

gauucaccuc cuuucucaga cggaagugga aauucaagaa agagcgcaau aagggcuaca     2220

aacaucacgc ugaagaugcc cugaucaucg cgaacgcuga cuuuauuuuc aaggagugga     2280

aaaagcugga uaaggccaaa aagguuaugg aaaaucagau guuugaggag aaacaagccg     2340

aaagcaugcc ugagaucgaa acagagcagg aauauaagga gauuuucauc acaccccauc     2400

agauuaaaca caucaaggac uuuaaggauu acaaauauuc ccauagagug gacaagaaac     2460

cuaaccggga gcuuauuaau gacacccucu acucuacgcg caaggaugac aaagggaaca     2520

cccugaucgu caauaaccug aauggacucu acgauaagga caacgauaag cugaagaaac     2580

uuaucaauaa aagccccgaa aagcugcuca uguaucacca cgacccccag acauaccaaa     2640

aacugaagcu gauuauggag caguauggcg augaaaagaa cccucuuuac aaauauuacg     2700

aggaaaccgg gaauuaucuc acaaaguacu ccaaaaagga caacggcccg gugaucaaaa     2760

agauuaagua cuauggaaau aaacugaacg cucaucugga uaucaccgac gauuacccca     2820

acucuagaaa uaaggugguu aaacucagcc ugaagccuua ucgguucgac gucuaccuug     2880

auaacggcgu guauaaauuc gugacgguca agaaucugga cgugauuaaa aaggagaacu     2940

acuaugaggu uaauuccaag ugcuacgaag aggccaaaaa gcucaaaaag aucagcaacc     3000

aggcugaauu uaucgccucu uucuacaaua acgaccugau uaaaaucaau ggggagcugu     3060

aucgcgugau uggagucaac aaugaucuuc ugaacagaau cgaggugaau augauugaca     3120

ucaccuaccg ggaauaucuc gagaacauga augauaagag acccccuaga aucauuaaga     3180

caaucgcguc caaaacccaa agcauuaaga aauacucuac agacauccug ggcaaccucu     3240

augaaguuaa guccaaaaag cacccccaga uuaucaagaa agggaagcgg ccggccgcua     3300

ccaaaaaggc cggacaggcu aaaaagaaga aauaaggacu agucccuguu cccagagccc     3360

acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa aaaaaaaaaa     3420

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu          3475


<210>  4717
<211>  4543
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4717
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcccu guucaacaag ugcauuauca uuagcaucaa ucucgacuuu uccaacaaag      180

agaaguguau gaccaagccg uacucuauug gacuggauau cggcacaaau agcguugggu      240

gggcggugau cacggacaac uauaaagugc ccuccaagaa aaugaagguc cuuggaaaua      300

ccucuaaaaa guacauuaag aaaaaccugc ucggcgugcu gcuuuucgau agcgggauca      360

cagcugaagg acggcgccug aagagaaccg cccggcgcag auauacacgg cgcagaaauc      420

ggauucucua ccugcaggag aucuuuucca ccgaaauggc cacacuggac gaugcuuucu      480

uucaacgccu cgacgauucu uuccugguuc cugacgauaa aagagacagc aaguauccca      540

uuuuuggcaa ccuuguggag gaaaaagucu accaugauga guucccuacc aucuaucauc      600

ugcggaagua ccucgccgac uccacgaaaa aggcugaucu gcgccuugug uaccuggccc      660

ucgcgcacau gauuaaguau agaggccacu uucugaucga gggggaguuc aauagcaaaa      720

acaaugacau ccagaagaac uuucaggacu uccuggauac cuacaaugcc auuuuugaau      780

cugaccucuc ccuggaaaac agcaaacagc uugaggagau cguuaaggau aaaauuucua      840

agcuggaaaa gaaagaccgg auccucaagc uguuccccgg agagaaaaau uccggcauuu      900

ucagcgaauu ucuuaagcug aucgugggga accaagcuga uuuccgcaaa ugcuuuaacc      960

ucgacgagaa ggccucucug cauuucucca aggaaagcua ugaugaggac cuggagacac     1020

uccugggaua caucggcgau gacuauuccg augucuuucu uaaagcuaag aaacuguacg     1080

acgccauucu ccugucuggg uuccuuaccg ugacagacaa ugaaaccgag gcgccucuga     1140

gcuccgccau gaucaagaga uauaacgaac acaaagagga ucucgcucug cugaaggaau     1200

acauucggaa uaucagccuc aagacguaca acgagguguu uaaagacgau acaaagaaug     1260

gauaugccgg cuacauugac ggcaaaacca accaggagga uuucuauguc uaccugaaga     1320

aucuucuggc ugaauuugag ggggccgacu auuuccucga aaagaucgau agagaggacu     1380

uucugcgcaa gcagcggaca uucgauaacg gaucuauccc guaccagauu caucugcaag     1440

aaaugagagc cauccuugac aaacaggcua aguuuuaucc cuuccucgcg aagaauaaag     1500

agcgcauuga gaagauccug accuuccgga uuccuuacua cguuggcccc cuggccagag     1560

ggaacuccga uuuugcuugg agcauccgca aacggaauga aaagauuacc ccuuggaacu     1620

ucgaggacgu gaucgauaaa gaaucuagcg ccgaggccuu uaucaauaga augacauccu     1680

ucgaccucua ucugcccgag gaaaaggugc uuccgaagca cucucugcuc uacgagacgu     1740

uuaacgucua uaaugaacug accaaagugc gcuucauugc ugagagcaug cgggacuacc     1800

aguuucugga uuccaagcag aaaaaggaca ucguuagacu uuauuucaaa gauaagcgca     1860

aggugacaga caaagauauu aucgaauacc uccacgccau uuauggauac gacggcaucg     1920

agcugaaggg gaucgagaaa caauuuaaca gcucucuguc caccuaccau gaucuccuga     1980

acauuaucaa ugacaaggag uuccuugaug acagcucuaa cgaagcgauu aucgaagaga     2040

uuauccacac acugaccauc uuugaagauc gggagaugau uaaacagaga cucuccaagu     2100

ucgagaauau cuuugacaag agcguccuga aaaagcuguc ccggcgccac uauacgggau     2160

ggggcaaacu uucugcuaag cucauuaacg gcaucagaga ugaaaagagc gggaauacaa     2220

uucuggacua ccugaucgac gauggaaucu ccaaccggaa uuucaugcag cucauucaug     2280

acgaugcccu gucuuucaag aaaaagaucc aaaaggccca gauuaucggc gacgaggaua     2340

aagggaacau uaaggaagug guuaaaagcc uuccuggauc ccccgcuauc aagaaaggca     2400

uucugcagag caucaagauc guggacgagc ucgucaaggu gaugggcggg agaaaaccug     2460

aaucuauugu cguggagaug gcccgcgaga aucaguauac caaccaagga aaguccaaua     2520

gccagcagcg gcugaaaaga cuggaaaagu cucuuaaaga gcucggcucc aagauccuga     2580

aggaaaacau ucccgcuaaa cugagcaaga ucgauaauaa cgcccuccag aaugaccgcc     2640

uguaccuuua uuaccugcaa aacgggaaag auauguauac cggagacgau cucgacauug     2700

acagacuguc uaacuacgau aucgaccaca ucauucccca ggcguuucug aaggauaaua     2760

gcaucgacaa caaaguucuu guguccagcg ccucuaaucg gggcaagucc gaugacuucc     2820

cuagccugga ggucgugaag aaacgcaaga cauuuuggua ccagcuccug aaauccaagc     2880

ucauuucuca gagaaaauuc gauaaccuga ccaaggcuga gcgcggggga cuucugccgg     2940

aagacaaggc cggcuuuauc caacggcagc ucguugagac aagacagauu acgaaacaug     3000

uggcucggcu gcuggaugaa aaguucaaua acaaaaagga cgagaauaac cgcgccguca     3060

gaaccgugaa aaucaucaca cuuaagagca cccugguguc ccaguuucgg aaagauuucg     3120

aacucuauaa gguucgcgag auuaaugacu uucaccaugc gcacgaugcc uaccugaacg     3180

cugucaucgc cucugcucuc cugaagaaau aucccaaacu ugagccugag uucguguacg     3240

gggacuaucc caaauacaau agcuuuagag aacggaaguc cgccacagaa aaaguguauu     3300

ucuacucuaa cauuaugaau aucuucaaaa agagcauuuc ccuggccgac ggcagaguca     3360

ucgagcgccc ucucauugaa gugaacgagg agaccggaga aagcguuugg aauaaagagu     3420

cugaucuggc uacggugcgg agaguccugu ccuaccccca agugaacguu gugaagaaag     3480

ucgaggaaca gaaccacggc cuugaccgcg ggaagccgaa aggacuguuu aaugcgaacc     3540

ucagcucuaa gccuaagccc aauuccaacg aaaaucuggu gggcgccaaa gaguaucucg     3600

auccuaagaa auacggggga uaugcuggca ucagcaacuc uuucgccguu cuggugaagg     3660

ggaccaucga gaaaggcgcc aagaagaaaa uuacaaaugu ccuugaauuu cagggaaucu     3720

ccauucugga ccggaucaac uacagaaagg auaaacugaa uuuccuccug gagaagggcu     3780

auaaagacau ugaacuuauc aucgagcugc ccaaguacag ccucuuugag cuguccgaug     3840

ggagccgccg gaugcucgcu ucuauucuga gcaccaacaa uaaaagagga gaaauccaua     3900

agggcaacca aauuuuccuu ucccagaagu uugugaaacu gcuguaucac gccaagcgca     3960

ucucuaauac aauuaacgag aaucaucgga aauacgugga aaaccacaag aaagaguucg     4020

aggaacucuu uuacuauauc cuggaguuca augaaaacua cgucggggcg aagaagaacg     4080

gaaaacuucu gaauagcgcu uuucaguccu ggcagaacca cucuaucgac gagcucugua     4140

gcuccuucau uggcccuacc gggucugaaa gaaagggccu guuugagcuc acgagccggg     4200

gauccgccgc cgauuucgag uuccugggcg uuaaaauccc ccgcuauaga gacuacacac     4260

cgagcucucu ucugaaggau gcuacccuga uucaucaauc cgugacaggg cucuaugaaa     4320

cccggaucga ccuggccaaa cuuggagagg gcaagcgccc ugcggcuacc aagaaagccg     4380

ggcaggccaa gaaaaagaaa uaaggacuag ucccuguucc cagagcccac uuuuuuuucu     4440

uuuuuugaaa uaaaauagcc ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                       4543


<210>  4718
<211>  3619
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4718
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccug guacgcgagc cugauguccg cucaucaccu ccggguugga auugacgugg      180

gcacccauuc uguggggcug gccacacuuc gcgucgauga ccacggaacg ccgaucgagc      240

ugcucagcgc ccugucccau auucacgauu cuggcguggg gaaggaaggg aaaaaggacc      300

acgauaccag aaagaaacuu agcggcaucg cucggcgcgc cagacggcug cuccaucacc      360

gcagaacaca gcugcaacag cuggacgagg uucuccggga ucugggcuuc cccauuccua      420

cccccgggga auuucuugac cugaacgagc agacagaucc uuaucgcgug uggagagucc      480

gggcucgccu cguggaagag aagcugcccg aggaacuuag aggaccugcc aucuccaugg      540

cgguucggca uaucgcccgc cacagaggcu ggcggaaucc guacucuaaa guggagagcc      600

ugcucucccc cgcugaagag agcccuuuca ugaaggcccu gagagagcgc auucuggcua      660

ccacagggga aguccucgac gauggaauca cccccggcca ggccauggcg caaguggccc      720

ugacgcacaa cauuucuaug cgggggccug agggaauccu uggcaaacug caucaguccg      780

acaaugcuaa cgaaauuaga aagaucugcg cccgccaggg cgugagcccc gaugucugua      840

agcagcuccu gcgggcuguu uuuaaagccg acucuccgag aggguccgcc gugagccgcg      900

uggcuccuga cccccuuccu ggacaaggcu cuuuccggag agcgcccaag ugcgaucccg      960

aguuucagcg cuuccggauu aucuccaucg ucgccaaucu gagaauuagc gaaaccaaag     1020

gggagaaccg cccucucaca gcugacgagc ggagacacgu gguuaccuuu cugacagaag     1080

auucccaggc cgaccugacc uggguggaug ucgccgagaa gcucggagug caucggcgcg     1140

accugagagg cacggcuguu cacacagaug acggggaacg gucugccgcg agaccgccca     1200

ucgaugcuac cgaccgcauu augcggcaga caaaaaucag cucccuuaag accugguggg     1260

aggaagccga uagcgagcaa agaggagcca ugauucgcua ucuguacgag gacccuaccg     1320

acucugaaug ugcugagauc aucgccgaac uccccgagga agaucaggcu aaacuggacu     1380

cccuucaucu gccugccggc agagcggccu auagccggga gucucucaca gcucugagcg     1440

aucacaugcu ggccacgacc gacgaucucc acgaggcucg caagagacug uucggcgugg     1500

acgauuccug ggcgcccccg gccgaagcca uuaaugcucc ugucgggaac cccucugugg     1560

accggacacu uaagaucguc ggacgcuacc ugagcgccgu ggaguccaug uggggcaccc     1620

cugaaguuau ucauguggag cacgucagag auggguuuac aagcgagcgg auggcugacg     1680

aacgcgauaa agccaauaga cggcgcuaua acgacaauca ggaggccaug aagaaaaucc     1740

aaagagauua cggaaaggaa ggcuacauuu cucggggaga caucgugaga cucgacgcuc     1800

uggagcugca gggcugcgcg ugucuuuauu gcggaaccac gaucggcuac cauacauguc     1860

agcucgauca cauuguuccc caggccgggc cuggauccaa caaucgccgg ggcaaccugg     1920

uggcugucug cgaaagaugu aaucgcagca aaucuaacac ccccuucgcc gugugggccc     1980

aaaagugcgg gaucccgcac gugggaguua aggaggcuau uggccggguc agaggguggc     2040

gcaaacagac cccuaacaca uccagcgagg accugacccg gcucaagaaa gaagugaucg     2100

ccagacugcg ccggacacag gaggaucccg aaauugacga gagauccaug gaaucugugg     2160

cguggauggc uaaugagcuu caucaccgga ucgccgccgc uuaucccgag acgaccguca     2220

ugguguaccg cggcagcauu acagccgcgg cuagaaaggc cgccggaauc gauucccgga     2280

ucaaccugau uggcgaaaaa gggcgcaagg acagaaucga ucggagacau cacgcuguug     2340

acgccucugu ggucgcucuc auggaggcca gcguggcgaa gacccuggcc gaacgcucca     2400

gccugcgggg agagcagaga cuuacaggca aagaacaaac cuggaagcag uauacggggu     2460

cuaccguugg agcucgcgag cauuuugaga uguggcgggg ccacaugcuc caccugacag     2520

aacuguucaa ugagagacuc gccgaagaua aaguguacgu cacccagaac auucgccugc     2580

ggcuuuccga cgggaaugcu cauacaguga acccuagcaa gcugguuucu cacagacucg     2640

gcgauggacu gaccgugcag caaaucgacc gcgccuguac gccggcccug uggugcgcuc     2700

uuacaagaga gaaagauuuu gacgagaaga auggccuccc cgcgcgggaa gaccgcgcca     2760

uuagagucca ugggcacgag aucaagucca gcgauuauau ccagguguuc ucuaaacgga     2820

agaaaaccga cagcgaucgc gacgaaacac cuuucggagc uauugccgug agaggcgggu     2880

uugucgagau cggacccucc auucaucacg cccggaucua cagaguugaa ggcaagaaac     2940

cuguguacgc uaugcugcgc guguucaccc acgaucugcu cagccagcgg cauggggacc     3000

uguuuucugc cgucauuccc ccucagucca ucagcaugag augcgcggag cccaaacuuc     3060

gcaaagcuau caccacaggc aacgccacgu aucugggaug ggugguugug ggcgaugagc     3120

ucgaaauuaa ugucgacucc uucaccaagu acgccaucgg gcgguuucug gaggauuucc     3180

cgaacacaac cagauggcgg auuuguggau augacacaaa uucuaagcug acccuuaaac     3240

cuaucgugcu cgcugccgaa ggccuggaga accccagcuc cgcgguuaau gaaauugugg     3300

agcugaaggg guggcgcguc gcuaucaacg ugcucacgaa aguucacccu acagugguca     3360

gacgggaugc ccugggacgc cccagauacu cuagccgguc caaucuuccg accucuugga     3420

ccaucgagaa gcgcccugcc gcuacaaaga aggccggcca agcuaagaaa aagaaauaag     3480

gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc     3540

uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaagaauu                                                  3619


<210>  4719
<211>  4480
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4719
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccac caagccguac agcauuggac uggacaucgg cacaaacucc guuggguggg      180

cggugacgac cgauaauuau aaagugcccu cuaagaagau gaaaguccuc ggaaacacaa      240

gcaagaaaua cauuaagaaa aaucugcuug gcgugcuccu guucgacucc gggaucaccg      300

cugagggacg gcgccugaag agaacagccc ggcgcagaua uacccggcgc agaaaccgga      360

uucuuuaccu gcaggaaauc uuuucuacag agauggccac ccucgaugac gcuuucuuuc      420

aacgccugga ugacagcuuc cugguuccug augacaagag agauuccaaa uaucccaucu      480

uuggcaaucu cguggaagag aaggccuacc augacgaguu cccuacgauu uaucaucugc      540

ggaaauaccu ugcugauucu accaagaaag ccgaccugcg ccucgucuac cuggcgcuug      600

cccacaugau caaguauaga ggccacuuuc ugauugaggg ggaguucaac agcaaaaaua      660

acgauaucca gaagaauuuu caggacuucc ucgacacaua caacgcuauu uuugaauccg      720

aucugagccu ggaaaauucu aagcagcucg aagagaucgu gaaagacaag auuuccaaac      780

uggagaagaa agaucggauc cuuaagcugu uccccggaga aaagaacagc ggcaucuuuu      840

cugaguuccu caaacugauu guugggaauc aagccgacuu ccgcaagugc uuuaaccuug      900

augaaaaggc uucccugcau uucagcaaag agucuuauga cgaagaucuc gagacccugc      960

ugggauacau cggcgacgau uauuccgacg uguuucucaa agccaagaag cuguacgaug     1020

cgauucuucu gagcggguuc cucacaguca ccgacaauga gacggaagcc ccucuguccu     1080

cugcuaugau caaaagauau aacgagcaca aggaagaccu ugcccugcuc aaagaguaca     1140

uucggaacau cagccugaag acauacaaug aaguguuuaa agaugacacc aagaacggau     1200

augcuggcua caucgauggc aagacaaauc aggaggacuu cuauguguac cugaaaaagc     1260

uccuggccga guuugaaggg gccgauuauu uccuugagaa aauugacaga gaagauuuuc     1320

ugcgcaagca gcggaccuuc gacaacggau ccaucccgua ccagauucac cuccaagaga     1380

ugagagcuau ccuggauaaa caggcgaagu uuuaucccuu ccuggccaaa aauaaggaac     1440

gcauugagaa gauccuuacc uuucggaucc cuuacuacgu cggcccccuc gcuagaggga     1500

acagcgacuu cgccuggucu auucgcaaac ggaaugagaa gaucacaccu uggaacuucg     1560

aagauguuau ugacaaagag uccagcgccg aggcuuuuau caauagaaug acgucuuucg     1620

aucuguaucu gcccgaagag aaggugcucc cgaaacauag ccugcuuuac gaaaccuuua     1680

acguguauaa ugagcugaca aagguccgcu ucauugccga auccaugcgg gacuaccagu     1740

uucucgacuc uaagcagaaa aaggauaucg ugagacugua uuucaaagac aagcgcaaag     1800

uuaccgauaa ggacauuauc gaguaccugc acgcgaucua uggauacgau ggcauugaac     1860

uuaaggggau cgagaaacaa uuuaacagcu cccucagcac auaccaugac cugcugaaua     1920

uuaucaacga uaaggaguuc cucgacgauu cuuccaauga agcuauuauc gaggaaauca     1980

uucacacccu gacgaucuuu gaggaccggg aaaugauuaa acagagacuu agcaaguucg     2040

agaacaucuu ugauaaaucu gugcugaaga agcucucccg gcgccacuau acaggauggg     2100

gcaaacugag cgccaagcug auuaacggca ucagagacga gaaauccggg aauaccaucc     2160

uugauuaccu cauugacgac ggaaucucua accggaauuu caugcagcug auucaugaug     2220

acgcccugag cuuuaagaaa aagauccaaa aagcucagau uaucggcgau gaagacaagg     2280

ggaacaucaa ggaggucgug aaaucccucc cuggaucucc cgccauuaag aaaggcaucc     2340

ugcagagcau uaagaucguu gaugaacuug ugaaagucau gggcgggaga aagccugagu     2400

ccauuguggu cgaaauggcu cgcgagaauc aguauaccaa ccaaggaaag agcaauucuc     2460

agcagcggcu gaaaagacuc gagaaguccc ugaaagaacu gggcagcaag auccuuaaag     2520

agaacauucc cgccaagcuc ucuaagaucg acaauaacgc gcugcagaau gaucgccugu     2580

accucuauua ccugcaaaac gggaaagaca uguauacagg agaugaccuu gauaucgaca     2640

gacuguccaa uuacgacauu gaucacauca uuccccaggc cuuccucaag gacaacagca     2700

ucgauaauaa agugcugguu ucuagcgcuu ccaaccgggg caagagcgac gaugugccuu     2760

cucuggaagu cgugaaaaag cgcaagaccu ucugguacca gcuucugaaa uccaagcuca     2820

uuagccagag aaaauuugac aaccugacaa aggccgagcg cgggggacuc uccccggagg     2880

auaaagcugg cuucauccaa cggcagcugg uugaaacgag acagaucacc aagcaugugg     2940

cccggcuucu ggacgagaag uuuaauaaca aaaaggauga aaauaaccgc gcggucagaa     3000

cagugaaaau uaucacccuc aagucuacac uggugagcca guuccggaaa gacuuugagc     3060

uguauaaggu ucgcgaaauu aaugauuucc accaugccca cgacgcuuac cuuaacgccg     3120

ucguggcuuc cgcccugcuc aaaaaguauc ccaagcugga gccugaguuu guguacgggg     3180

auuaucccaa auacaauucu uucagagaaa gaaagagcgc caccgagaaa gucuauuuuu     3240

acuccaacau caugaauauu uucaagaaau cuaucagccu cgcugacggc cgggugaucg     3300

aacgcccucu gauugagguu aacgaagaga cgggagaguc cguguggaau aaggaaagcg     3360

accuugcgac cguccggaga gugcugucuu acccccaagu uaacgugguc aagaaagugg     3420

aggaacagaa ucacggccuc gaucgcggga agccgaaagg acuguuuaac gccaaucugu     3480

ccagcaagcc uaaacccaac ucuaacgaga aucuuguugg cgcuaaggaa uaucuggacc     3540

cuaagaaaua cgggggauau gccggcaucu ccaacagcuu cacagugcuc gucaagggga     3600

ccauugagaa aggcgccaag aaaaagauca caaaugugcu ggaguuccag ggaauuucua     3660

uccucgaucg gauuaacuac agaaaggaca aacugaauuu ucuucuggaa aagggcuaua     3720

aagauaucga gcugaucauu gaacucccca aguacucccu guucgagcuu agcgacgggu     3780

cccgccggau gcuggcuagc auccucucua ccaacaauaa aagaggagag auucauaagg     3840

gcaaccaaau cuuucugagc cagaaauucg ugaagcuccu guaucacgcc aagcgcauuu     3900

ccaauacgau caacgaaaau caucggaaau acgucgagaa ccacaagaaa gaauuugagg     3960

aacuuuucua cuauauccug gaguuuaaug agaacuacgu uggggcgaag aaaaauggaa     4020

agcugcucaa cucugcuuuc cagagcuggc agaaucacuc cauugaugaa cuguguucua     4080

gcuuuaucgg cccuacaggg uccgagagaa agggccuuuu cgaacugacc ucucggggaa     4140

gcgccgccga cuuugaguuc cucggcguga aaauuccccg cuauagagau uacacaccgu     4200

ccagccugcu caaggacgcu acccugaucc aucaaucugu gaccgggcuu uaugaaacac     4260

ggauugaucu ggccaaacug ggagagggca agcgcccugc ggcuacgaaa aaggccgggc     4320

aggccaagaa aaagaaauaa ggacuagucc cuguucccag agcccacuuu uuuuucuuuu     4380

uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                           4480


<210>  4720
<211>  3562
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4720
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc ggcuuucaag ccgaacagca uuaauuacau ccugggacuc gacauuggca      180

ucgccuccgu ugggugggcc augguggaga uugaugaaga ggaaaacccc auccggcuga      240

ucgaccuugg agugcgcguc uuugagagag cugaagugcc uaaaaccggc gauucucugg      300

ccauggcucg gcgccucgcc agaagcguuc ggcgccugac aagacggcgc gcgcauagac      360

uucugcggac gcgcagacuc cugaagcggg agggggugcu gcaggccgcu aauuucgacg      420

agaacggacu cauuaagucc cugcccaaua ccccuuggca acuucgcgcc gcugcccugg      480

auagaaaacu cacaccccug gaauggucug cgguccuucu gcaccucauc aagcaucggg      540

gcuaucugag ccagcgcaaa aacgagggcg aaaccgccga caaggagcug ggggcucucc      600

ugaaaggagu ggccggcaau gcucacgccc uucagacagg ggauuuuaga accccugccg      660

agcuggcucu caacaaguuc gaaaaggagu ccggacauau ucggaaucag agaucugacu      720

acagccacac auuuucccgc aaagaucugc aagcggaacu uauccuccug uucgagaagc      780

agaaagaauu uggcaacccg cacguuagcg ggggacugaa ggagggcauu gagacccugc      840

ucaugacgca gcggcccgcc cugucuggcg acgcugugca gaaaaugcuu gggcauugca      900

ccuucgaacc ugccgagccc aaggccgcua aaaauacaua uaccgccgaa agauuuaucu      960

ggcugacaaa gcucaacaau cugcgcauuc uugagcaagg auccgaacgg ccucugaccg     1020

auacggagag agcgacacuc auggacgagc ccuaccgcaa gagcaaacug accuaugcuc     1080

aggcccggaa gcugcucggc cuggaagaua cagccuucuu uaaagggcuu agauacggaa     1140

aggacaacgc ugaggccucu acccugaugg aaaugaaggc uuaucacgcc aucucccgcg     1200

cgcucgagaa ggaaggccug aaggacaaaa agagcccgcu gaaucuuucu ccugagcucc     1260

aggaugagau cgggaccgcc uucucccugu uuaaaacaga cgaagauauu acgggacggc     1320

ugaaggacag aauccagccc gagauucucg aagcucugcu uaaacauauc agcuucgaua     1380

aguucgucca aauuucccug aaggcccucc ggcgcaucgu gccucugaug gagcagggca     1440

aaagauacga cgaggcuugu gccgaaaucu acggcgauca cuaugggaag aaaaacaccg     1500

aggaaaagau uuaccugccu cccauccccg cggacgagau ucggaacccg gugguccuua     1560

gagcccucuc ucaggcucgc aaaguuauca auggaguggu gcggagauau ggcagccccg     1620

cccgcauuca caucgaaaca gcuagagagg ucgggaaguc cuuuaaggau cggaaagaga     1680

ucgaaaagcg ccaagaggaa aacagaaaag accgggagaa ggccgccgcu aaauuccgcg     1740

aauacuuucc uaauuucgug ggagagccca agagcaaaga uauucugaag cugagacucu     1800

augagcagca gcauggcaag ugccuguacu cugggaaaga aaucaaccuu ggccggcuga     1860

augagaaggg auauguugaa auugaccacg cgcucccuuu uucccgcacc ugggacgaua     1920

gcuucaacaa uaaagugcug guccugggcu cugagaacca gaauaagggg aaccaaacac     1980

ccuacgaaua cuuuaaugga aaagacaaca gcagagagug gcaggaguuc aaggcccggg     2040

uggaaaccuc ccgcuuuccg agaucuaaga aacagcggau ccuucuccag aaguucgaug     2100

aggacggcuu uaaagaaaga aaucugaacg auacgcgcua uguuaaucgg uuccuguguc     2160

aauuuguggc ugacagaaug cgccucacag ggaagggcaa aaagcggguc uucgccagca     2220

acggacagau uaccaaccug cuuagagggu ucuggggccu gcgcaaggug cgggccgaga     2280

augauagaca ucacgcucuc gacgccgucg ugguugcgug cuccaccgug gcuaugcagc     2340

agaaaaucac acgcuuuguc cgguacaagg agaugaacgc cuucgaugga aaaaccauug     2400

acaaggaaac aggcgaggug cugcaucaga aaacgcacuu uccucagccc ugggaguucu     2460

uugcccagga aguuaugauc agaguguucg ggaagccuga uggaaagccc gaauuugagg     2520

aggcugacac ccuggaaaaa cuucggacac uccuggccga gaagcugagc ucucgcccug     2580

aagcggucca cgaguaugug acaccccucu ucguguccag agcuccgaau cggaaaauga     2640

gcggccaggg gcauauggaa accguuaagu cugccaaacg ccuggaugag ggagucuccg     2700

ugcuuagagu gccucugacc caacucaagc ugaaagaccu ggagaagaug gucaaccggg     2760

aaagagagcc caaacuuuac gaagcccuga aagcucgccu cgaggcccac aaggacgauc     2820

ccgcuaaagc cuuugcggaa ccuuucuaua aguacgacaa agccggcaau cggacgcaac     2880

aggugaaggc uguuagagug gagcaagucc agaagaccgg gguguggguu cgcaaccaua     2940

auggcaucgc cgauaacgcu acaauggugc gggucgacgu guuugagaaa ggagauaagu     3000

auuaccuggu uccgauuuac agcuggcaag uggccaaagg cauccucccc gacagagccg     3060

ucgugcaggg gaaggaugaa gaggacuggc agcugauuga ugacuccuuc aauuuuaaau     3120

ucucucuuca cccuaacgau cugguggaag ucaucaccaa gaaggcucgc auguucggau     3180

auuuugcgag cugucaccgg ggcacaggga auauuaacau cagaauccau gaccucgauc     3240

acaaaauugg aaagaauggc auccuggagg ggauuggcgu uaaaaccgcc cuguccuucc     3300

agaaguacca gaucgacgag cuuggaaaag aaauucgccc cugcagacug aagaagcggc     3360

cucccgugcg caaaagaccu gcugccacga agaaagccgg ccaggcuaag aaaaagaagu     3420

aaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu     3480

gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaagaa uu                                              3562


<210>  4721
<211>  4318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4721
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gaaaccguac accauuggac uggacaucgg cacaaacagc guuggguggg      180

cggugcucac ggaucaguau gaccugguga agcggaagau gaaaauugcu ggagauuccg      240

agaagaaaca aaucaagaaa aauuucuggg gcguccgccu uuuugacgaa gggcagaccg      300

ccgccgauag acggauggcu cgcacagcca gacggcgcau ugagagacgg cgcaacagaa      360

ucucuuaccu gcagggaauc uucgcugaag agaugagcaa gaccgacgcc aauuucuucu      420

gccggcucuc cgauucuuuu uauguggaca acgaaaagcg caauagcaga caucccuucu      480

uugcgacaau ugaggaggaa guugaguacc acaaaaacua uccuaccauc uaccaucugc      540

gggaggaacu ugucaauucc ucugagaagg ccgaucugcg ccucgucuau cuggcucugg      600

cccacauuau caaauacaga ggcaacuucc ucauugaagg cgcucuggac acacagaaua      660

ccagcgugga ugggaucuac aagcaauuua uucagacgua uaaccagguu uucgccuccg      720

gaaucgagga cggcagccuu aaaaagcugg aagauaauaa agacguggcg aagauccucg      780

ucgagaaggu gacccggaaa gaaaagcugg agcgcauucu uaaacuguac cccggggaga      840

agucugccgg aauguuugcu caguucaucu cccucauugu gggcagcaaa gggaacuuuc      900

aaaagccuuu cgaccugauc gaaaagucug auauugagug ugccaaagac uccuaugaag      960

aggaucugga aagccuccug gcucuuaucg gagacgagua cgccgagcug uucgucgccg     1020

cuaagaaugc guauucugcc guugugcucu ccagcaucau uacaguggcu gaaaccgaga     1080

caaacgccaa acuguccgcc ucuaugaucg aaagauuuga uacccaugag gaagaccuug     1140

gcgagcugaa ggcuuucauu aaacuccauc ugcccaagca cuacgaggaa aucuuuagca     1200

auacggagaa gcacggcuau gccggguaca uugauggaaa aacaaagcag gcggacuucu     1260

acaaauauau gaagaugacc cuggaaaaca ucgagggcgc ugauuacuuu aucgccaaaa     1320

uugagaagga aaacuuccuc cggaagcaga gaacauuuga caauggggcc aucccucacc     1380

agcugcaucu ugaggaacug gaggcuauuc uccaccaaca ggccaaauau uacccguucc     1440

ugaaggaaaa cuaugauaaa aucaaguccc uugucaccuu ucgcauuccc uacuucgugg     1500

gaccucuggc uaauggccag agcgaguuug ccuggcucac ccggaaagcg gacggggaga     1560

ucagacccug gaacauugaa gagaagguug acuucggaaa aucugccgug gauuuuaucg     1620

aaaagaugac aaauaaggac acguaucugc cuaaagagaa cguccugccc aagcacuccc     1680

ucugcuacca aaaauaccug guguauaaug aacuuaccaa gguucgcuac aucaacgauc     1740

agggcaaaac aagcuauuuc ucuggccagg agaaggagca gauuuucaau gaccuguuua     1800

agcaaaaacg gaaggugaaa aagaaagauc ucgaacuguu ccugagaaac augagccaug     1860

ucgagucccc gaccaucgaa gggcuugagg acucuuuuaa uagcuccuac agcacauauc     1920

acgaucuccu gaagguggga auuaagcagg aaauccugga caacccuguc aauaccgaga     1980

ugcucgagaa cauugugaaa auccugacgg uuuucgaaga uaagcgcaug aucaaagagc     2040

agcuucagca auuuucugac gugcuggaug gcgucgugcu caagaaacug gaacggagac     2100

auuacacagg guggggacgc cuguccgcua agcuccucau gggcauucgg gacaagcaga     2160

gccaccugac cauccuggau uaucucauga augacgacgg gcugaacaga aaccuuaugc     2220

agcugauuaa ugauucuaac cucuccuuca aaagcaucau ugagaaggag cagguuacca     2280

cagccgacaa agauauccaa uccaucgugg cugaccuggc cggcucuccc gcgauuaaga     2340

aaggaauccu gcagagccuu aagauugucg augaacucgu guccgugaug ggcuacccuc     2400

cccagaccau cguugucgag auggcccggg aaaaucagac aacggggaaa ggaaagaaca     2460

auucucgccc cagauacaag agccuggaga aagcuauuaa ggaauuuggc ucccaaaucc     2520

ugaaagagca uccuaccgac aaccaggagc uccggaauaa cagacuguau cuuuacuauc     2580

ugcagaaugg gaaggauaug uacacaggac aagaccucga uauccacaac cugagcaauu     2640

augacauuga ucacaucgug ccgcagucuu ucauuaccga caacuccauc gauaaucugg     2700

ugcuuacaag cucugccggc aaccgcgaaa aaggggacga cgugcccccu cucgagauug     2760

ugcggaagag aaagguguuu ugggaaaaac uguaccaggg caaucugaug uccaagcgca     2820

aauucgauua ucucaccaag gcugagagag gaggccugac ggaagccgac aaagcccggu     2880

uuauccaucg ccagcuuguu gagaccagac aaauuacaaa gaacgucgcu aacauccugc     2940

accagcgguu caauuacgag aaggaugacc augggaacac caugaaacag gugcgcaucg     3000

uuacacucaa gagcgcgcug gugucucagu uuagaaaaca auuccagcug uacaaggucc     3060

gggaugugaa ugacuaucac cacgcccaug augcuuaccu uaacggaguu guggccaaua     3120

cccugcucaa agucuauccc cagcuggaac cugaguucgu guacggcgac uaucaccagu     3180

uugauugguu caaggccaac aaggcuacgg ccaaaaagca auuuuacaca aauauuaugc     3240

uuuucuuugc gcagaaagac cgcaucauug augaaaacgg ggagauccug ugggacaaga     3300

aauaucuuga caccgugaag aaggucauga gcuacagaca gaugaauauu guuaaaaaga     3360

cagaaaucca aaaaggagag uucuccaagg cuaccaucaa acccaagggc aacagcucua     3420

aacugauucc gcggaagacc aauugggauc cuaugaagua cgggggccuc gacuccccca     3480

acauggccua ugccguggug aucgaguacg cuaaaggaaa gaauaaacug gucuuugaaa     3540

agaaaauuau ccgcgugaca auuauggaga gaaaggccuu cgaaaaggau gagaaagcgu     3600

uucuggagga acagggcuau cggcagccua agguucuugc uaaacugccc aaguacacgc     3660

ucuaugagug ugaagagggg agacgccgga ugcuggccag cgccaacgaa gcucagaaag     3720

gaaaucaaca ggugcucccu aaccaucugg ucacccuucu gcaccaugcc gcuaacugcg     3780

agguguccga cggcaagucu cucgauuaca ucgagagcaa uagagaaaug uucgccgagc     3840

ugcuggcgca cguuuccgaa uuugccaagc gcuauacacu ugcugaggcc aaccugaaua     3900

aaaucaacca gcucuuugaa cagaauaagg agggggacau uaaagcuauc gcccaaucuu     3960

ucguggaucu gauggccuuu aacgcuaugg gagcgcccgc cagcuucaag uuuuucgaga     4020

ccacaauuga acggaaaaga uacaauaacc ucaaggagcu gcuuaauucc accaucauuu     4080

accagucuau cacgggccug uaugaaagcc gcaagcggcu ggacgauaaa agaccggcug     4140

ccacaaagaa agccgggcag gcuaagaaaa agaaauaagg acuagucccu guucccagag     4200

cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu       4318


<210>  4722
<211>  4351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4722
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gaaaccguac agcauuggac uggacaucgg caccaacucc guuggguggg      180

cgguggugac agaugacuau aagguccccg cuaagaaaau gaaggugcuc ggaaauacgg      240

auaaaucuca uauugagaag aaccugcuug gcgcccuccu guucgacagc gggaauaccg      300

ccgaagaucg gcgccugaaa agaacagcuc ggcgcagaua cacccggcgc agaaaccgga      360

uccuuuaucu gcaggagauu uucuccgaag agaugggaaa gguugacgau ucuuucuuuc      420

accgccucga agacagcuuc cuggugacag aggauaagag aggcgagcgg cauccuaucu      480

uuggcaaucu ggaagaggaa gucaaauacc acgagaacuu ccccaccauc uaucaucucc      540

gccaauaccu ggccgacaau ccugagaagg uggaucuuag acugguuuau cucgcucugg      600

cccacauuau caaauuucgg gggcacuucc uuauugaagg aaaguuugac acacgcaaca      660

augaugugca gagacuguuc caggaguuuc ucgcggucua cgacaacacc uucgaaaauu      720

ccucucugca ggagcaaaac guccaggugg aagagauccu gacggacaaa auuagcaagu      780

ccgccaaaaa ggaucgggug cucaagcugu uucccaauga gaaaagcaac ggcagauucg      840

cugaauuucu uaagcugauc guugggaauc aggccgacuu uaaaaagcau uucgagcucg      900

aagagaaagc uccucugcag uuuucuaagg auaccuacga agaggagcuu gaagugcugc      960

ucgcccaaau uggagacaac uaugcggagc uguuccuguc cgccaagaaa cucuacgaua     1020

gcauccugcu uucuggcauc cugacaguga ccgacgucgg gacaaaggcu ccgcucuccg     1080

ccagcaugau ucagcgcuau aacgaacacc agauggaucu ggcucagcuu aaacaauuua     1140

uccggcagaa gcugucugac aaauacaaug agguguucuc cgauguuagc aaggacggau     1200

augccggcua cauugauggc aagaccaacc aggaagccuu uuauaaauac cucaaggggc     1260

ugcugaauaa aaucgaggga uccggcuacu uccucgacaa gauugagaga gaagacuuuc     1320

ugcgcaaaca acggacguuc gauaacgggu cuauccccca ucagauccac cuucaggaga     1380

ugagagcuau uauccgccgg caggcggaau uuuauccuuu ccuggccgac aaucaagaua     1440

gaauugagaa gcuccugaca uuucgcaucc ccuacuaugu gggaccucug gcucggggca     1500

agagcgacuu cgccuggcuu uccagaaaaa gcgccgauaa gauuaccccc uggaacuucg     1560

acgagaucgu cgauaaagaa ucuuccgcug aggccuuuau caaucgcaug acaaacuacg     1620

accucuaucu gccgaaucag aaggugcugc cuaaacacag ccuccuguac gaaaaguuca     1680

ccguuuauaa cgagcuuacc aaagugaagu acaagacaga acaggggaaa acggcguuuu     1740

ucgaugcuaa uaugaagcag gagauuuuug acggagucuu caaaguguac cggaagguca     1800

ccaaagauaa gcugauggac uuucucgaga aggaguucga ugaauuuaga aucguggacc     1860

ugacaggccu ggacaaagaa aacaagguuu ucaaugccuc uuauggcacc uaccaugauc     1920

uuugcaaaau ucucgacaag gauuuucugg acaacagcaa aaaugagaag auccuggaag     1980

auauugugcu cacacugacc cuuuucgagg accgggagau gauccgcaag agacuggaaa     2040

acuauuccga ucuccugacg aaagagcaag ucaagaaacu ggaacggaga cacuacacag     2100

gguggggacg ccuuucugcc gagcucauuc auggcauccg gaacaaggaa agcagaaaaa     2160

ccauccugga cuaucugauu gaugacggaa auuccaaccg caauuuuaug cagcucauca     2220

acgaugacgc ucugagcuuc aaggaggaga uugccaaggc ucaggugauc ggggaaaccg     2280

auaaucuuaa ccagguugug ucugacauug ccggcucccc cgcgaucaaa aaggggaucc     2340

ugcaaagccu caaaauuguc gacgagcugg ugaagaucau gggccaccag ccugaaaaua     2400

uugugguuga gauggccaga gagaaccagu ucacaaauca gggacggcgc aacucucaac     2460

agagacugaa aggccuuacc gauuccauca aggaauuugg gagccagauu cucaaagagc     2520

aucccgucga aaauucccaa cugcagaacg accggcuguu ccucuacuau cugcagaaug     2580

gacgcgauau guacacaggc gaggaacuug acaucgauua ccugucucag uaugacaucg     2640

aucacauuau cccccaggcu uuuauuaagg acaacagcau cgauaauaga gugcucacgu     2700

ccucuaagga gaaccggggg aaaagcgacg augugccuuc caaggacguc gugcgcaaaa     2760

ugaaaagcua cuggucuaaa cugcuguccg ccaaacuuau uacccaaaga aaguucgaca     2820

accugacaaa agcugagcgg ggaggccuca ccgaugacga uaaggccggg uuuaucaaac     2880

gccagcuggu ugaaacaaga cagaucacca agcacguggc gcggauucuc gacgagagau     2940

ucaauacgga aaccgaugag aacaauaaaa agauccgcca agucaagauu gugacacuga     3000

aaagcaaccu uguuucuaau uuucggaagg aguucgaacu guauaaagug agagagauca     3060

acgacuacca ucacgcccau gaugcuuauc ucaaugccgu cauuggcaag gcucugcugg     3120

gaguguaccc gcagcuugaa cccgaguuug uuuauggcga cuacccucac uuccacgggc     3180

auaaagaaaa caaggccacc gccaagaaau uuuucuacuc caauaucaug aacuucuuua     3240

agaaagauga cgugcgcaca gauaagaaug gagagauuau cuggaaaaag gacgaacaca     3300

ucagcaacau uaagaaaguc cugucuuauc cccaggugaa uaucgugaag aaagucgagg     3360

agcagaccgg cggguucagc aaggaaucca uucucccuaa aggaaacagc gauaagcuga     3420

ucccccggaa aacgaagaag uuuuacuggg acacaaagaa guauggcggg uucgacucuc     3480

cgauuguugc uuacuccauc cucgugaucg cggauauuga gaaaggcaag agcaaaaagc     3540

ugaagaccgu gaaagcccuu gucggaguga caaucaugga aaagaugacc uuugagagag     3600

acccuguugc uuuccuggag cgcaaaggcu aucggaaugu gcaagaagag aacauuauca     3660

agcuccccaa auacucccug uuuaagcugg aaaacgggag aaagcgccuu cuggccucug     3720

cccgggagcu ccagaaagga aaugaaauug uccugccuaa ccaucucggc acccugcuuu     3780

aucacgcuaa gaauauccau aaaguggaug agcccaagca ccuggacuac guugauaaac     3840

acaaggacga guucaaggaa cugcucgaug uggucagcaa cuuuuccaaa aaguacacac     3900

uggccgaggg gaaucuugaa aagaucaagg agcuguaugc gcagaacaau ggagaagacc     3960

ucaaagagcu ggcuucuagc uucauuaacc uccugacguu uaccgccauc ggcgccccug     4020

cuacauucaa guuuuucgau aaaaauauug acagaaagcg guacaccucc acaaccgaga     4080

uccuuaacgc cacgcugauu caucaaucua ucacagggcu guaugaaacc cgcauugauc     4140

ucaauaagcu gggcggagac aaaagacccg cggcuaccaa gaaagccggc caggccaaga     4200

aaaagaagua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu uuuugaaaua     4260

aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                                    4351


<210>  4723
<211>  3484
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4723
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccca gacaacaaac cugagcuaca uucucggacu ggaccuuggc aucgcguccg      180

uugggugggc ugugguggag auuaaugaaa acgaggaucc gaucggacug auugacgucg      240

gcgugcggau cuucgaacgc gccgagguuc ccaagacggg ggaaucucuc gcccugagca      300

gacggcuugc ucgcuccacc agacggcuga uccgcagacg ggcccaucgc cuccugcugg      360

cuaaaagauu ucucaagcgg gaggggauuc ugucuacaau cgaucuugag aagggccugc      420

cuaaucaagc cugggaacuc cgcguggcgg gccuggagag acggcuuagc gccauugaau      480

ggggagcugu ccugcuccac cugaucaaac aucgcggaua ucuguccaag cggaaaaacg      540

agucucagac caauaacaag gagcucggcg cccugcuuag cgggguggcu cagaaucacc      600

agcugcucca auccgacgau uacagaacac ccgccgaacu ggcgcuuaaa aaguucgcca      660

aggaggaagg acauauuaga aaccagcgcg gcgcuuauac ccacacauuu aaucggcugg      720

accuccuggc cgagcugaac cuccuguucg cucagcagca ccaauuuggg aauccucauu      780

gcaaagaaca cauccagcag uacaugaccg agcuucugau guggcagaag cccgcccuca      840

gcggagaggc cauucugaaa augcuuggca aguguacgca ugaaaagaac gaguucaaag      900

cugcgaaaca caccuauucu gccgaaagau uuguuuggcu gacaaagcuc aauaaccugc      960

gcauccugga ggauggcgcu gaacgggccc ucaaugagga ggaaagacaa cugcuuauca     1020

accacccuua cgagaagucc aaacugaccu augcccaggu gcgcaagcuc cuggggcuga     1080

gcgaacaggc uauuuucaaa caucuucggu acucuaagga gaacgccgaa uccgcgacau     1140

uuauggagcu caaggcuugg cacgccauca gaaaagcccu ggagaaucaa ggacugaagg     1200

acaccuggca ggaucucgcu aaaaagccgg accugcuuga ugaaauuggc acggccuuca     1260

gccuguacaa aacagacgag gauauccagc aguaucucac caacaagguc cccaauucug     1320

ugauuaacgc ucugcuggug ucccuuaauu uugacaaauu caucgaacuc agccugaagu     1380

cccugcgcaa gauccucccu cugauggagc aagggaaacg guacgaccag gccugcagag     1440

agauuuaugg acaucacuac ggcgaagcga accagaagac aucucagcuu cugcccgcca     1500

ucccugcuca agagauucgc aaucccgucg uucuccggac ccugagccag gccagaaaag     1560

ugaucaacgc uauuauccgg caguaugggu ccccggcccg cgugcauauc gaaaccggaa     1620

gagagcuggg caagagcuuu aaagaacgga gagagauuca gaagcaacag gaggauaauc     1680

gcacaaagcg ggaaucugcg guccagaaau ucaaggagcu uuucuccgac uuuagcucug     1740

aaccuaaaag caaggauauc cucaaauuca gacuguacga gcagcaacac ggcaaguguc     1800

uguauuccgg gaaagaaauu aacauccacc gccucaauga gaagggauac guggagauug     1860

accaugcccu gcccuuuucu agaacguggg augacagcuu caacaauaag guucuugugc     1920

uggcuuccga aaaccagaau aaaggcaacc agaccccuua cgaguggcuc caagggaaga     1980

ucaacagcga acgguggaaa aauuuugucg cccuggugcu gggaucucag ugcuccgcug     2040

ccaagaaaca gcgccuucuc acacagguua uugaugacaa caaguucauc gauagaaauc     2100

ugaacgacac ccgguauauc gcccgcuuuc ugagcaauua cauucaagag aaccuccugc     2160

uugugggcaa gaauaaaaag aacgucuuca cacccaaugg gcagaucacc gcucugcuca     2220

gaucucggug gggccugauu aaagcgcgcg aaaacaauaa cagacaccau gcccuggaug     2280

cuaucguggu cgccugugcc acgcccucca ugcagcagaa gauuacacgg uuuauccgcu     2340

ucaaagaggu gcacccuuau aagaucgaga auagauacga aaugguugac caagagagcg     2400

gagaaauuau cuccccgcac uuucccgagc cuugggcuua uuuccggcag gaggugaaca     2460

uuagagucuu ugacaaucau cccgauaccg ugcuuaagga aaugcugccu gaccgccccc     2520

aggccaacca ccaguucguu caaccucucu ucgugucucg ggcgccgacc agaaaaauga     2580

gcggccaggg gcauauggag acaaucaagu ccgcuaaacg ccuggccgaa ggaauuucug     2640

uccuccggau cccccugacc cagcuuaagc cuaaccugcu cgagaauaug gugaacaaag     2700

aaagagagcc cgcccuguac gcuggccuga aggcccgccu ugcggaguuu aaucaagauc     2760

cugcuaaggc cuucgccaca cccuuuuaua aacaaggggg acaacaggug aaggcuaucc     2820

ggguugaaca gguccagaaa agcggcgugc uggugagaga gaacaauggg gucgccgaca     2880

acgcuuccau ugugcgcacg gauguuuuca ucaagaauaa caaauuuuuc cucgugccga     2940

uuuacaccug gcaggucgcc aagggcaucc ugccuaauaa agcgauugug gcccacaaga     3000

acgaagacga gugggaagag auggaugagg gagcuaaguu uaaauucagc cucuuuccca     3060

augaccuggu ugaacuuaag accaaaaagg aguacuucuu uggcuauuac aucgggcugg     3120

aucgggccac aggaaacauc ucucucaaag aacaugacgg cgagauuucc aaggggaagg     3180

auggagugua uagagucggc gugaaacugg cucugagcuu cgaaaaguac cagguugacg     3240

agcuugggaa aaaucggcag aucugccgcc cccagcagag acagccugug cggaagcgcc     3300

cggccgccac caaaaaggcu ggccaagcga agaaaaagaa auaaggacua gucccuguuc     3360

ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca gaucuaaaaa     3420

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag     3480

aauu                                                                  3484


<210>  4724
<211>  3562
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4724
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc ggcuuucaag ccgaacccca uuaauuacau ccugggacuc gacauuggca      180

ucgccagcgu ugggugggcc augguggaga uugaugaaga cgagaacccu aucugccuga      240

ucgaucuugg agugcggguc uuugaacgcg cugaggugcc caaaaccggc gacucccugg      300

ccauggcuag acggcucgcc cgcucuguua gacggcugac acgcagacgg gcgcaucgcc      360

uucugagagc ccggcgccuc cugaagagag aaggggugcu gcaggcugcc gauuucgacg      420

agaauggacu cauuaagagc cugccuaaca cgcccuggca acuucgggcu gccgcgcugg      480

aucgcaaacu caccccucug gagugguccg ccguccuucu gcaccucauc aagcauagag      540

gcuaucuguc ucagcggaaa aaugaaggcg agacagcuga caaggaacug ggggcccucc      600

ugaaaggagu ggcugauaac gcccacgccc uucagaccgg cgacuuuaga acaccggcug      660

agcuggcgcu caauaaguuc gagaaggaaa gcgggcauau ucgcaaccag cggggagauu      720

acucccacac cuuuucuaga aaagaccugc aagccgagcu uauccuccug uucgaaaagc      780

agaaagaguu uggcaauccc cacguuagcg ggggacugaa ggaaggcauu gagacacugc      840

ucaugaccca gcgcccugcu cuguccggcg augccgugca gaaaaugcuu gggcauugua      900

cguucgagcc cgccgaaccu aaggcugcca aaaacaccua uacagcggag cgguuuaucu      960

ggcugaccaa gcucaauaac cugcgcauuc uugaacaagg aagcgagaga ccccugacag     1020

acaccgaacg ggcuacgcuc auggacgagc cguacagaaa gucuaaacug acauaugccc     1080

aggcccgcaa gcugcucggc cuggaggaua ccgcuuucuu uaaagggcuu cgguacggaa     1140

aggacaaugc cgaggcuucc acacugaugg aaaugaaagc cuaucacgcg aucagcagag     1200

cccucgaaaa ggagggccug aaggauaaaa agucuccucu gaaccuuucc cccgaacucc     1260

aggacgagau cgggaccgcu uucagccugu uuaaaaccga ugaggacauu acaggacgcc     1320

ugaaggaucg gauccagccu gaaauucucg aggcccugcu uaaacauauc ucuuucgaca     1380

aguucgucca aauuucccug aaggcucuca gacggaucgu gccccugaug gaacagggca     1440

aacgcuacga ugaggccugc gcggagaucu acggcgacca cuaugggaag aaaaauacgg     1500

aagagaagau uuaccugccc ccuaucccgg ccgaugaaau uagaaacccc gugguccuuc     1560

gggcucucag ccaggccaga aaaguuauca acggaguggu gcgccgguau ggcuccccug     1620

cucgcauuca caucgagacc gccagagaag ucgggaaguc uuuuaaggac agaaaagaga     1680

ucgagaagcg gcaagaagag aaucgcaaag acagagaaaa ggccgcugcg aaauuccggg     1740

aguacuuucc caacuucgug ggagaaccua agagcaaaga uauucugaag cugcgccucu     1800

augagcagca gcauggcaag ugucuguacu ccgggaaaga gaucaaucuu ggcagacuga     1860

acgaaaaggg auauguugag auugaccacg cccuccccuu uagccggaca ugggaugacu     1920

cuuucaauaa caaagugcug guccugggcu ccgaaaauca gaacaagggg aaucaaaccc     1980

cguacgagua cuuuaacgga aaagauaaua gccgcgaaug gcaggaguuc aaggcuagag     2040

uggagacauc ucgguuuccu cgcagcaaga aacagagaau ccuucuccag aaguucgacg     2100

aagauggcuu uaaagagcgg aaccugaaug acaccagaua uguuaaccgc uuccugugcc     2160

aauuuguggc cgaucggaug agacucacgg ggaagggcaa aaagcgcguc uucgccucca     2220

auggacagau uacaaaccug cuucgggggu ucuggggccu gagaaaggug cgcgcugaaa     2280

acgaccggca ucacgcccuc gaugcggucg ugguugcuug uucuaccgug gccaugcagc     2340

agaaaaucac cagauuuguc cgcuacaagg agaugaaugc cuucgacgga aaaacaauug     2400

auaaggagac cggcgaagug cugcaucaga aaacacacuu uccccagccu ugggaguucu     2460

uugcucagga aguuaugauc cggguguucg ggaagcccga cggaaagccu gaguuugaag     2520

aggccgacac gcccgagaaa cugagaaccc uucucgcgga aaagcugagc ucccggccgg     2580

aggcugucca cgaauaugug acaccucugu ucgugagccg cgcccccaac agaaaaaugu     2640

cuggccaggg gcauauggag accguuaagu ccgccaaacg gcucgaugaa ggagucagcg     2700

ugcugcgcgu gccccuuaca caacugaagc ucaaagaccu ggagaagaug gucaauagag     2760

agcgggaacc uaagcuguac gaggcucuua aagccagacu ggaagcucac aaggaugacc     2820

cggccaaagc guuugccgag cccuucuaua aguacgauaa agcuggcaac cgcacccaac     2880

aggugaaggc cguucgggug gaacaagucc agaagacggg gguguggguu agaaaucaua     2940

acggcaucgc ugacaaugcc accauggugc gcgucgaugu guuugagaaa ggagacaagu     3000

auuaccucgu uccuauuuac ucuuggcaag uggccaaagg cauccugccc gaucgggcug     3060

ucgugcaggg gaaggacgag gaagauuggc agcucauuga cgauuccuuc aacuuuaaau     3120

ucagccugca cccuaaugac cuuguggagg ucaucacaaa gaaggcgaga auguucggau     3180

auuuugccuc cugccaccgc ggcaccggga acauuaauau ccggauccau gaccuggauc     3240

acaaaauugg aaagaacggc auccucgaag ggauuggcgu uaaaacagcu cugucuuucc     3300

agaaguacca gaucgacgag cugggaaaag agauuagacc cugucgccuu aagaagagac     3360

cucccgugcg gaaacgcccg gccgccacca agaaagcugg ccaggccaag aaaaagaagu     3420

aaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu     3480

gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaagaa uu                                              3562


<210>  4725
<211>  3568
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4725
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa guaccauguu ggaauugacg ugggcaccuu cagcgugggg cuggcggcua      180

ucgaggucga ugacgccgga augccgauua aaacacucuc ccuggugucu cacauccaug      240

auagcggccu ugaccccgau gaaauuaagu ccgccguuac gcggcuggcu ucuagcggga      300

ucgcccgcag aacccggcgc cucuauagac ggaagcgcag acggcugcag caacuugaca      360

aauuuaucca gcgccaggga uggccuguga uugagcugga agauuacucc gacccccucu      420

auccuuggaa ggucagagcu gagcuggccg cgucuuacau cgccgaugaa aaagagcggg      480

gcgagaagcu gagcguggcu cuccgccaca uugccagaca ucggggcugg cgcaaccccu      540

augcuaaagu uuccagccug uaccuuccug acgggccguc ugaugccuuc aaggcgauca      600

gagaagagau uaagcgggcc uccggacagc ccgugccuga aacagcuacc gucggccaaa      660

uggugacacu gugcgagcuc gggacccuga aacuuagagg agagggcggg gugcugagcg      720

cccgccucca gcagucugac uaugcucggg aaauccagga gauuuguaga augcaagaaa      780

ucggacagga gcuguaccgc aagaucauug augucguuuu ugccgccgaa ucccccaaag      840

gcagcgcuuc uucccgggug ggcaaggacc cucugcagcc cgggaaaaau agagcgcuca      900

aggccagcga cgcuuuccag cgcuaccgga ucgccgcccu gauuggaaac cuuagagugc      960

gcgucgaugg cgagaaacgg auccuguccg uggaggaaaa gaaucucguu uuugaccacc     1020

uggugaaccu uacaccgaag aaagagccug aaugggucac cauugcugag auccugggga     1080

ucgauagagg acaacucauu ggcacggcca ccaugacaga cgauggggaa cgcgcgggag     1140

cucggccccc uacccacgac acaaauagau cuaucgugaa cagccggauu gcuccccugg     1200

uugauuggug gaagaccgcc uccgcccugg agcagcaugc cauggugaaa gcucucagca     1260

augccgaggu cgacgauuuc gacucucccg aaggcgcgaa ggugcaggcc uuuuucgcug     1320

aucuggacga cgauguccac gccaaacuug acucccugca ucucccugug ggccgcgcug     1380

ccuauagcga ggauacgcug guuagacuua cacggagaau gcugucugac gggguggauc     1440

ucuacaccgc gcgccugcaa gaauuuggaa ucgagccgag cuggacaccc ccuacacccc     1500

ggauuggcga accugucggg aaccccgccg uggacagagu ucugaagacc gugucccgcu     1560

ggcucgaguc ugcuaccaag acguggggag ccccggagag agucaucauc gaacacgugc     1620

gggagggcuu cgugaccgaa aaacgcgcua gagagaugga uggggacaug cggcgcagag     1680

ccgcccggaa ugcuaagcug uuucaggaga ugcaggaaaa acuuaacguu cagggcaagc     1740

cuagccgcgc ggaucugugg agauaucaau ccguccagcg gcagaauugc cagugugccu     1800

acugcggaag ccccauuaca uucucuaacu ccgagaugga ccacaucgug ccucgcgcug     1860

gccaagggag caccaauaca agagaaaacc ucguggccgu cugucaucgg ugcaaucagu     1920

cuaaaggaaa cacccccuuu gccauuuggg cuaagaacac guccaucgag ggcgugagcg     1980

uuaaggaagc cguggagaga acacgccacu gggucaccga uaccgggaug cgguccacag     2040

acuucaaaaa guucaccaaa gcggugguug agagauuuca gcgcgcuaca auggaugaag     2100

agauugacgc ccggucuaug gaaagcgugg ccuggauggc uaaugagcug agaucccgcg     2160

ucgcccagca uuucgcgucu cacggaacga ccgugcgggu uuauagaggc agccugacag     2220

cugaagcccg ccgggccucc gggaucagcg gcaagcugaa auuuuucgac ggagugggca     2280

agucuagacu cgaucggcgc caucacgcua uugacgccgc ugucaucgcc uuuaccuccg     2340

auuacguggc ggagacacug gccgugagaa gcaaccugaa gcaaucucag gcucaccggc     2400

aggaggcccc ucaguggcgc gaauuuaccg ggaaagacgc ugagcauaga gccgccuggc     2460

gggucuggug ucaaaagaug gaaaaacucu ccgcucugcu uacggaggau cugagagacg     2520

aucgcguugu ggugaugagc aauguccggc ucagacuggg aaacggcucu gcgcacaagg     2580

aaaccaucgg gaaacugagc aaggugaaac uuuccagcca gcucucuguu uccgacauug     2640

auaaggccag cuccgaggcu cuguggugcg cccugacacg cgagcccgga uuugacccga     2700

aggaaggccu cccugccaau cccgagcggc auaucagagu gaacgggacc cacgucuaug     2760

cuggcgauaa uauuggacug uuccccgugu cugccggcag caucgcgcuu cgcgggggau     2820

acgcugaacu gggcuccucu uuucaccaug cccggguuua caaaauuaca agcgggaaga     2880

aaccugccuu cgcuaugcuc agaguguaua ccaucgaccu gcugccguac cgcaaccagg     2940

aucuuuucuc cgucgagcuc aagccccaaa cgaugucuau gagacaggcc gagaaaaagc     3000

ugcgggacgc gcuggcuaca ggaaaugccg aauaucucgg cuggcuggug guugacgaug     3060

agcuuguggu cgacaccagc aagaucgcca cagaucaggu gaaagcugug gaagccgagc     3120

uggggaccau ucgcagaugg cgggucgacg gcuucuuuuc cccuagcaag cuccgccuga     3180

gaccccugca gaugucuaaa gaaggaauca agaaagaguc cgcuccugag cuuagcaaga     3240

uuaucgaucg gcccggcugg cucccugccg uuaacaagcu guucucugac gggaauguga     3300

ccguggucag acgcgauucc cugggacggg ugagacucga aagcacagcg caccuucccg     3360

uuacguggaa agugcaaaag cgcccggccg cuaccaaaaa ggccggccag gcuaaaaaga     3420

agaaauaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa     3480

uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        3568


<210>  4726
<211>  3268
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4726
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc gcggauucug gcuuucgaca ucggaauuag cuccaucggc ugggccuuuu      180

cugagaacga ugaacucaag gacugcgggg uucgcauuuu caccaaagug gagaauccga      240

agacaggaga aagccuggcc cuucccagac ggcuggcucg cuccgccaga aagcggcucg      300

cucgcagaaa agcccggcug aaccaucuua agcaccugau cgcgaaugag uuuaaacuca      360

acuacgaaga uuaucagucu uucgacgaga gccuggccaa ggcuuacaaa ggcucccuga      420

ucucuccuua ugagcuccgc uuuagagccc ugaaugaacu ucugagcaag caagauuucg      480

cucgggugau ucuccauauc gccaagcgca gaggguacga cgauauuaaa aacuccgacg      540

auaaggagaa aggagcgauc cugaaggcca uuaaacagaa ugaagagaaa cuugcuaacu      600

aucagagcgu cggcgaguac cuguauaaag aauacuuuca gaaguucaag gaaaauucua      660

aagaauuuac gaacgugcgg aauaagaaag aguccuacga acgcuguauc gcccagagcu      720

uccucaagga cgagcugaaa cugauuuuua agaagcaaag agaguucggc uuuucuuucu      780

ccaaaaaguu cgaagaggaa guucucagcg uggcuuuuua uaaacgggcc cugaaggauu      840

ucucucaccu ugucgggaac ugcuccuuuu ucaccgacga gaaaagagcc cccaagaaua      900

gcccucuggc uuuuauguuc guggcgcuca cacgcaucau caaccugcuu aauaaccuga      960

agaacaccga aggcauucuc uacacaaaag augaccugaa ugcccugcuc aacgagguuc     1020

ugaagaaugg aacccuuaca uauaaacaga ccaagaaacu gcucgggcug uccgacgauu     1080

acgaguuuaa gggagaaaag ggcacguauu ucaucgaguu uaaaaaguac aaagaguuca     1140

uuaaggcucu uggggagcau aaccugucuc aggacgaucu caaugaaauc gccaaagaca     1200

uuacccugau caaggaugag aucaaacuga agaaggcccu cgcuaaauau gaccugaacc     1260

aaaaucagau ugauagccuu uccaagcugg aguuuaaaga ccaccucaac aucagcuuca     1320

aggcccugaa acuggugaca ccccuuaugc ucgaaggaaa gaaguacgau gaggcgugua     1380

augaacugaa ccugaaaguc gcuauuaaug aggacaagaa agauuuucuc ccugccuuca     1440

acgagaccua cuauaaggac gaagugacaa auccgguggu ccugcgggcc aucaaagagu     1500

acagaaaggu ucuuaacgcu cugcucaaga aauauggcaa ggugcacaaa auuaauaucg     1560

aacuggcccg cgaggugggc aagaaccauu cucagcgggc uaaaaucgaa aaggagcaga     1620

acgagaauua caaggccaaa aaggacgcgg aacuggagug cgaaaaacuu gggcucaaga     1680

uuaacuccaa aaauauccug aagcugagac ucuucaaaga gcaaaaggaa uuuugugccu     1740

auagcggaga gaagauuaaa aucucugauc ugcaggacga gaagaugcuu gaaauugauc     1800

acaucuaccc cuauagccgc uccuucgacg auucuuacau gaacaaaguc cugguguuua     1860

ccaagcagaa ucaagagaaa cucaaccaga cgccuuucga ggcuuuuggc aaugacagcg     1920

ccaaguggca gaagauugaa guucuggcua aaaaccugcc cacaaagaaa cagaagcgga     1980

uccuugauaa aaauuacaag gacaaggaac aaaagaacuu caaggauaga aaucucaacg     2040

acacccgcua uaucgcccgg cuggugcuga auuacacaaa agauuaucuc gacuuucugc     2100

cucuuuccga ugacgagaac accaagcuga augacaccca gaaagggagc aagguccaug     2160

uggaggcgaa gucuggaaug cucacauccg cccugagaca cacguggggc uucagcgcua     2220

aagaucgcaa caaccaucug caccacgcca uugacgcugu uaucauugcc uacgccaaua     2280

acucuaucgu gaaggcuuuu uccgauuuca aaaaggaaca ggagagcaau uccgcggaac     2340

uuuaugccaa aaagauuucu gagcucgacu acaagaacaa acggaaguuu uucgagcccu     2400

uuagcggguu cagacagaaa guccuggaua agaucgacga aaucuucgug uccaaaccgg     2460

agcggaagaa accuucugga gcucugcaug aagagaccuu ucgcaaggaa gaggaguucu     2520

aucaaagcua cggcggcaag gaaggggucc ucaaagcccu ggagcuugga aagauuagaa     2580

aagugaaugg caagaucguu aaaaacgggg auauguuucg gguggacauu uucaagcaca     2640

agaaaacaaa uaaguuuuac gccgucccca ucuauaccau ggauuucgcu cugaaagugc     2700

ucccuaacaa ggccguugcg agauccaaaa agggagaaau uaaggacugg auccugaugg     2760

augagaauua cgaauuuugc uucagccugu auaaagacuc ucuuauccug auucagacaa     2820

aggauaugca ggagcccgag uuuguguacu auaacgcuuu caccuccagc acggucucuc     2880

ucaucguguc caaacaugac aauaaguuug aaacacugag caaaaaccag aagauucucu     2940

ucaagaaugc caacgagaaa gaagugaucg ccaagucuau uggcauccaa aaucugaaag     3000

uuuucgagaa guacaucguc agcgcucuug gggaagugac caaagccgag uuucgccagc     3060

gggaggacuu caagaaaaag agacccgcgg cuaccaagaa agccggccag gccaagaaaa     3120

agaaauaagg acuagucccu guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa     3180

uagccugucu uucagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaagaauu                                        3268


<210>  4727
<211>  3679
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4727
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccag cgaccugguu cucggacugg auauuggcau cggguccgug ggagugggca      180

uucuuaacaa ggucaccggg gagaucauuc auaaaaauuc ucggaucuuc ccggcggcuc      240

aggccgaaaa caaucuggug cgcagaacaa accggcaagg acgcagacuc gcccggcgca      300

agaagcacag acggguucgc cugaauagac uuuuugagga aagcggccug aucacggacu      360

ucaccaaaau uuccaucaac cucaaucccu accagcugcg ggugaagggc cugacagaug      420

agcucucuaa cgaggagcug uuuauugcuc uuaaaaauau ggucaagcau cgcgggauca      480

gcuaucugga cgaugccucc gacgauggaa acucuagcgu gggcgacuac gcucagauug      540

uuaaagaaaa uuccaagcag cucgaaacca agacaccugg gcaaauccag cuggagagau      600

aucagaccua cggacagcuu cggggcgauu ucacagugga aaaagacggg aagaaacacc      660

gccugauuaa cgucuuuccc accagcgccu auagaucuga ggcgcuccgg auccugcaaa      720

cgcagcagga guucaauccu cagaucaccg augaauuuau uaacagauac cuggagaucc      780

ucacaggaaa gcgcaaauau uaccauggcc ccggcaauga aaagucccgg accgacuacg      840

ggagauaucg cacaagcgga gagacccugg auaacauuuu cggcauccuu auugggaaau      900

gcacguuuua cccugacgag uuccgggccg cuaaggccuc uuauacagcu caagaauuua      960

accugcucaa ugaccugaac aaucuuaccg ugccgacaga gaccaagaaa cuguccaagg     1020

aacagaaaaa ccagaucauc aauuacguga agaacgagaa agccauggga cccgcgaaac     1080

ucuucaagua uauugccaaa cugcugagcu gugaugucgc ugacaucaag ggcuacagaa     1140

uugauaaauc ugggaaggcc gaaauccaca ccuuugaggc uuaucgcaaa augaagacac     1200

ucgaaacgcu ggacauugag caaauggauc gggagacccu ugacaagcug gccuacguuc     1260

ucacacugaa uaccgaaaga gagggaaucc aggaagcccu ugagcacgag uucgcugaug     1320

gcuccuucag ccagaaacag guggacgaac uggugcaauu ucgcaaggcg aacuccucua     1380

ucuucggcaa aggguggcau aauuuuagcg ucaagcucau gauggagcug auuccugaac     1440

uguacgagac auccgaggaa cagaugacca uccucacgcg gcugggaaaa cagaagacaa     1500

ccucuucuuc caacaagacc aaauauauug augagaaacu ucugacagaa gagaucuaca     1560

aucccguggu ugccaagagc gugagacagg cuauuaagau cgucaacgcc gccauuaaag     1620

aauauggcga cuucgauaau aucgugaucg agauggcucg cgaaaccaac gaggacgacg     1680

agaagaaagc cauucaaaag auccagaagg cgaauaaaga ugaaaaggac gcugccaugc     1740

ucaaagccgc uaaccaguac aacgggaagg ccgagcugcc ucacucuguu uuucauggac     1800

acaaacagcu ggcuacaaag auucggcuuu ggcaccaaca gggcgaaaga ugccucuaua     1860

cggggaagac caucagcauu caugaucuga ucaauaacuc caaucaguuc gagguggacc     1920

acauccugcc ccucucuauu acauuugaug acagccuggc caacaaaguc cuuguguacg     1980

cgaccgccaa ucaggaaaag ggccaacgga caccguauca ggcucuggau uccauggacg     2040

augccuggag cuuccgcgag cucaaggcuu uugucagaga gucuaagacc cuguccaaca     2100

aaaagaagga auaccugcuu acggaggaag acaucagcaa auucgaugug cggaagaaau     2160

uuauugagag aaaucucguu gacacccgcu acgccucucg ggugguccug aacgcgcugc     2220

aggaacauuu cagagcccac aagaucgaua caaaaguguc cguugugcgc ggacaauuua     2280

ccagccagcu cagacggcau uggggcauug agaagacacg cgacaccuau caccaccaug     2340

cugucgacgc ccugaucauc gcugccuccu cucagcuuaa ucuguggaag aaacagaaga     2400

acacgcucgu gagcuacucc gaggaucaac ugcuggacau ugaaacaggg gagcuuaucu     2460

cugaugacga auauaaagag agcguguuca aggccccuua ccagcacuuc guugauaccc     2520

ucaaauccaa ggaguuugaa gacagcauuc uguucucuua ucaggucgau uccaaauuua     2580

auagaaagau cagcgacgcu acaauuuacg cgacccggca ggccaaggug ggaaaagaua     2640

aggcugacga gaccuaugug cugggcaaaa ucaaggauau cuacacacaa gacggguacg     2700

augccuucau gaaaauuuau aagaaggaca aaucuaaguu ucucauguac cgccaugacc     2760

cccagacguu cgaaaaaguc aucgagccua uucuggaaaa cuaucccaau aagcagauca     2820

acgagaaagg aaaggaggug cccuguaauc cuuuucuuaa guacaaagag gaacacggcu     2880

auauuagaaa guacuccaaa aaggggaacg gcccggaaau caaaagccug aaguauuacg     2940

auucuaagcu cggaaaccac auugacauca cccccaaaga uagcaauaac aagguugugc     3000

ugcaguccgu cagcccuugg cgggccgacg uguacuucaa uaaaacaacc ggcaaguaug     3060

agauccuggg gcuuaaauac gcugaucucc aauuugaaaa gggaacaggc accuauaaga     3120

uuucucagga gaaauacaac gacaucaaga aaaaggaggg gguugauucc gacagcgagu     3180

ucaaauuuac gcuguauaag aaugaucugc uccuggugaa agacacagaa accaaggaac     3240

agcaacuuuu ccgcuuucug uccagaacca ugcccaagca gaaacauuac gucgagcuca     3300

agccuuauga uaaacagaag uucgaaggag gcgaggcccu gauuaaagug cuggggaacg     3360

uugcgaauag cggccagugc aagaagggac uuggcaaaag caacaucucc auuuacaagg     3420

ugcggacaga cguccugggg aaucaacaca ucaucaaaaa cgagggagac aagcccaaac     3480

ucgauuucaa gcgcccggcu gccaccaaga aagccggcca ggcuaagaaa aagaaauaag     3540

gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc     3600

uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaagaauu                                                  3679


<210>  4728
<211>  4237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4728
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccac ccaguucgag ggauuuacaa accuguacca aguuagcaag acgcuccggu      180

ucgaacugau uccgcagggc aaaacccuua agcauaucca ggagcagggg uuuauugaag      240

aggacaaggc gcgcaaugau cacuauaaag aacugaagcc caucauugac agaaucuaca      300

aaacauaugc ugaucaaugc cuccagcugg ugcagcuuga cugggagaac cuguccgccg      360

ccaucgauuc uuaccggaag gagaaaaccg aagagacacg caaugcucuc auugaagaac      420

aggccaccua uagaaacgcu auccaugacu acuucauugg acggacagau aaucugaccg      480

acgccaucaa caagcgccac gcggagauuu auaagggccu guuuaaagcc gaacucuuca      540

augggaaggu gcugaaacaa cuuggaacgg ucaccacaac cgagcaugaa aacgcucugc      600

ucagauccuu ugauaaguuc acaaccuacu uuagcggcuu cuacgagaau cggaaaaacg      660

uguuuucugc cgaagacauc agcacggcua uuccucaccg caucguucag gauaauuucc      720

ccaaguuuaa agagaacugu cacaucuuca caagacugau uaccgccgug ccuucccuuc      780

gggagcauuu cgaaaauguc aagaaggcga ucggcauuuu ugugucuaca agcaucgagg      840

aaguuuucuc cuuucccuuc uauaaccagc ugcucaccca gacccaaauu gaccuguaca      900

aucagcugcu cgggggaauc agccgcgagg ccggcacaga gaaaaucaag gggcugaacg      960

aggugcuuaa ccuggcuauu cagaaaaaug augagacggc ccacaucauu gcuucucucc     1020

cucauagauu uaucccgcug uucaagcaaa uucuuuccga ccggaacacc cugagcuuua     1080

uccucgaaga guucaaaucu gacgaagagg ucauccaguc cuuuugcaag uauaagacac     1140

ugcugcgcaa ugaaaacgug cucgagaccg ccgaggcccu guucaaugaa cuuaacagca     1200

uugaucugac acacaucuuu auuucucaca aaaagcucga gaccaucucc agcgcucugu     1260

gugaccauug ggauacgcuu agaaaugcgc uguacgaacg gagaauuucc gagcucacag     1320

gaaaaaucac caagucugcc aaagagaagg ugcagcgcag ccugaagcac gaagacauua     1380

accugcagga gaucaucucc gcugccggca aagaacucag cgaggccuuc aagcagaaaa     1440

ccucugaaau ucugucccau gcucacgccg cgcuugauca accccugccu acaacccuca     1500

agaaacagga ggagaaggaa auccuuaaga gccagcugga cucucuucuc gggcuguauc     1560

aucugcucga uugguuugcu gucgacgaga gcaaugaagu ugaucccgag uucuccgccc     1620

ggcugacagg aauuaaacuu gaaauggagc cuucucugag cuucuacaac aaggccagaa     1680

auuaugcuac gaaaaagccc uacuccgugg agaaauuuaa gcucaacuuc caaaugccga     1740

cccuggccag cggcugggac gugaauaaag aaaagaacaa uggcgcuauc cuguuuguca     1800

agaacgggcu uuacuaucuc ggaauuaugc cuaaacagaa gggccgcuac aaagcccugu     1860

cuuucgagcc cacagaaaag accuccgagg gguuugauaa aauguauuac gacuauuucc     1920

cugacgcggc caagaugauc cccaagugca gcacacagcu gaaagcugug accgcccacu     1980

uucagacgca caccacaccc auccuccugu cuaacaauuu cauugaaccu cuugagauca     2040

ccaaggagau uuacgaucug aacaauccgg aaaaagagcc caagaaauuu caaacagcuu     2100

augccaagaa gaccggagac cagaaaggcu accgggaagc gcucuguaag uggaucgauu     2160

ucacgagaga cuuucugucc aaauacacaa agaccacaag cauugaucug uccucucuuc     2220

gcccuagcuc ccaguauaaa gaccucgggg aguacuaugc cgagcugaac ccccugcucu     2280

accauaucuc uuuccagcgg aucgcugaaa aggagauuau ggaugccguu gaaaccggaa     2340

agcuguaucu uuuucaaauc uacaauaaag acuucgcuaa gggccaccau ggcaaaccua     2400

accugcacac ccucuauugg acagggcugu ucagccccga gaaucuggcc aagacgucca     2460

uuaaacuuaa cggacaggcc gaacucuuuu acagaccuaa gagccgcaug aaacggaugg     2520

cucacagacu gggcgagaag augcugaaua agaaacucaa ggaucagaaa accccgaucc     2580

ccgacacacu guaccaagag cuuuaugauu acgugaacca ucgccugucu cacgaccucu     2640

ccgaugaagc gcgggcccug cugccuaaug ucauuaccaa ggaggugagc caugaaauca     2700

ucaaagacag acgguucaca ucugacaagu uuuucuuuca cguucccauu acccuuaacu     2760

aucaggcugc caauuccccu agcaaguuca accagcgcgu gaaugccuac cugaaagagc     2820

aucccgaaac gccgaucauu gggaucgaua gaggagagcg gaaccucauu uauaucacag     2880

ucauugacuc uaccggcaag auccuggagc agagaagccu caacaccauc caacaguuug     2940

auuaccagaa aaagcuggac aaucgcgaaa aagagcgggu ggcugccaga caggcguggu     3000

ccgucguggg gacaauuaag gaucuuaagc aaggcuaucu gagccagguu auccacgaaa     3060

uuguggaccu caugauccac uaccaggcug ucgugguucu ggagaaccug aauuucggau     3120

uuaaaucuaa gcgcaccggc auugccgaaa aagccgugua ccagcaauuc gagaagaugc     3180

uuaucgauaa acugaacugc cucguccuga aggacuaucc ugcugagaag guugggggag     3240

ugcucaaucc cuaccagcug acagaucagu uuacguccuu cgccaaaaug ggcacccaaa     3300

gcggguuccu guuuuaugug cccgcgccuu acacauccaa gaucgacccg cugaccggau     3360

ucgucgaucc cuuugugugg aaaacaauua agaaccauga aucuagaaaa cacuuccucg     3420

agggcuuuga cuuccugcau uaugauguga agaccgggga cuuuauccug cacuucaaga     3480

ugaaucggaa ccuuagcuuu cagcgcggcc ugccuggauu caugcccgcu ugggacauug     3540

ucuuugagaa aaaugaaacg caguucgaug ccaagggcac cccuuuuauc gccgggaaaa     3600

gaauugugcc cguuaucgag aaccaucggu ucacaggacg cuacagagac cucuauccug     3660

cuaaugaacu gaucgcccuc cuggaggaaa agggcauugu guuccgggau ggguccaaca     3720

uccuucccaa acugcuggag aaugacgauu cucacgcuau ugacaccaug gucgcccuca     3780

uccgcagcgu gcugcagaug agaaacucca augcggccac aggagaagau uacauuaacu     3840

cuccgguucg ggaccuuaac ggcguguguu uugauagccg cuuccaaaau ccugaguggc     3900

ccauggacgc ugaugccaac ggggcuuacc acaucgcccu gaagggccag cuccugcuca     3960

aucaucugaa agaguccaag gaccuuaagc ugcagaacgg aauuagcaau caggacuggc     4020

uggccuauau ccaagaacuc agaaacaaac ggccugcugc gaccaagaaa gccggccagg     4080

cuaagaaaaa gaaguaagga cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu     4140

gaaauaaaau agccugucuu ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                              4237


<210>  4729
<211>  4216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4729
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccag cauuuaccag gaguucguua acaaguauuc ccugucuaaa acccuccggu      180

uugaacugau cccgcaagga aagacacuug agaauauuaa ggcgcgcggc cugauccucg      240

acgaugaaaa gagagcuaag gacuacaaaa aggccaaaca gauuaucgau aaguaucauc      300

aguucuuuau cgaggaaauu cugagcuccg ugugcaucuc ugaggaccuu cugcagaacu      360

acagcgaugu guauuucaag cucaaaaagu ccgacgauga caaucugcaa aaagauuuua      420

agucugccaa agacacgauu aagaaacaga ucagcgagua cauuaaggau uccgaaaagu      480

ucaaaaaccu guuuaaucag aaccucaucg acgcuaagaa agggcaggag agcgaucuga      540

uucuuuggcu gaagcaaucu aaagacaaug gaaucgaacu cuucaaggcc aacuccgaca      600

ucaccgauau ugacgaggcu cuggagauca uuaagagcuu uaaaggcugg acaaccuauu      660

ucaagggguu ucacgaaaau cggaaaaacg ucuacucuuc caaugauauc cccacaagca      720

uuaucuaccg caucguggac gauaaccuuc cuaaguuccu ggagaauaaa gccaaguaug      780

aaucucucaa ggacaaagcg cccgaggcca uuaacuacga acagaucaag aaagaucugg      840

cugaggagcu gaccuuugac auugauuaua agacauccga aguuaaucag agaguguuca      900

gccucgacga ggucuucgaa aucgccaacu uuaacaauua ccugaaccag uccggaauua      960

ccaaauucaa uacgaucauc ggcggcaagu uugugaacgg ggagaauacc aagcggaaag     1020

gaauuaacga auauaucaau cuuuacucuc aacagauuaa cgauaagaca cugaaaaagu     1080

auaaaaugag cguucucuuc aagcagaucc uguccgacac cgagagcaaa ucuuuuguga     1140

uugacaaacu ugaggaugac uccgaugucg ugacaaccau gcagagcuuc uacgaacaaa     1200

ucgcugccuu uaagacggug gaggaaaaau cuauuaagga gacacugagc cuccuguucg     1260

acgaucugaa agcgcagaag cucgaccugu ccaaaaucua cuuuaagaau gauaagucuc     1320

uuaccgaccu gagccagcaa gucuucgaug acuauuccgu uaucggcaca gccgugcucg     1380

aauacauuac ccagcagauc gcuccuaaaa accuggauaa ucccagcaag aaagagcagg     1440

agcuuauugc caagaaaacc gaaaaggcua aguaucuguc ucucgagaca aucaaacugg     1500

cccuggaaga guuuaacaag caucgcgaca uugauaaaca auguagauuc gaggaaaucc     1560

ucgccaauuu ugcugcgauc ccuaugauuu ucgacgagau cgcccagaac aaggacaauc     1620

uggcucagau uuccaucaaa uaccagaacc aagggaagaa ggaucuucug caggccagcg     1680

ccgaagacga ugugaaagcu auuaaggacc uccuggauca gacgaacaau cugcuucaca     1740

aacucaagau cuuccauauc ucucaguccg aggacaaagc caacauucug gauaaggacg     1800

aacacuuuua ucuggucuuc gaggagugcu acuuugaacu cgcgaauauc gugccgcugu     1860

auaacaaaau ucggaauuac aucacccaaa agcccuacag cgaugagaag uucaaacuua     1920

acuuugaaaa uuccacacug gcuaacggau gggacaagaa uaaagagccu gauaacaccg     1980

ccauucucuu caucaaggac gauaaauauu accugggcgu uaugaauaag aagaacaaua     2040

aaaucuuuga cgacaaggcc auuaaagaaa acaaggggga gggauauaaa aagaucgugu     2100

acaagcugcu ucccggcgcu aauaaaaugc ucccuaaggu cuucuuuucu gccaaaagca     2160

uuaaguucua uaaccccucc gaggauaucc ugcgcauuag aaaccacucu acacauacca     2220

aaaauggcag cccgcagaag ggguacgaaa aguuugaguu caacaucgaa gacugucgga     2280

aauuuauuga uuucuauaag caguccauca gcaaacaccc ugaguggaag gacuucggau     2340

uucgcuucuc ugauacgcaa agauacaauu ccaucgacga auuuuaccgg gagguggaga     2400

accagggcua uaaacugaca uucgaaaaua uuagcgaguc uuacaucgau uccguuguga     2460

accaggggaa gcucuaucug uuucagauuu acaauaagga cuucagcgcu uauucuaaag     2520

gacgccccaa ccuucauacc cuguacugga aggcccucuu ugaugaaaga aaucugcaag     2580

acgucgugua uaaacugaac ggcgaggcgg agcuuuucua ccggaagcag agcaucccua     2640

aaaagauuac ccaccccgcc aaagaagcua ucgccaauaa gaacaaggau aaucccaaaa     2700

aggaguccgu cuuugaauac gaccucauca aagacaagag auucacagag gauaaauuuu     2760

ucuuccacug cccuauuacc aucaacuuua agagcucugg ggcuaauaag uucaacgacg     2820

aaauuaaccu gcugcucaaa gagaaggcca augaugugca uauccugucc auugaccgcg     2880

gagagcggca ccuugcguau uacacacugg uugauggcaa aggcaacauc aucaagcagg     2940

acacguuuaa uauuaucggg aacgauagaa ugaaaaccaa uuaucaugac aagcucgccg     3000

cuauugaaaa ggaucgcgac agcgcccgga aagauuggaa gaaaaucaac aauauuaagg     3060

agaugaaaga aggauaccug ucccaggugg uccacgagau cgcuaagcug gugauugaau     3120

auaacgccau cguuguguuc gaggaccuua auuuuggcuu caagagaggg cgcuuuaaag     3180

ucgagaagca aguguaccag aaacucgaaa agaugcugau cgagaaacug aacuaucucg     3240

uguucaagga uaaugaauuu gacaaaacag gaggcguucu gcgggccuac cagcuuaccg     3300

cuccguucga gacauuuaag aagaugggga aacagaccgg cauuaucuac uauguccccg     3360

cgggauucac gucuaagauu uguccuguga ccggcuuugu gaaccaacug uaccccaaau     3420

augaaagcgu cuccaagucu caggaguucu ucagcaaauu ugacaagauc ugcuacaauc     3480

ucgauaaggg guauuucgag uuuuccuucg acuacaaaaa cuuuggagau aaggccgcua     3540

aaggcaagug gacaauugcc ucuuucggga gcagacugau caauuuucgc aacuccgaca     3600

aaaaucauaa cugggauacc cgggaagugu auccuacaaa ggagcuggaa aaacuucuga     3660

aagacuacag caucgaguac ggacacggcg aguguauuaa ggccgcuauc ugcggggaau     3720

cugauaaaaa guucuuugcc aaacucaccu ccguucugaa cacgauucuc cagaugagaa     3780

auagcaagac aggcaccgag cuggacuauc uuaucucucc cguggcggau gucaacggaa     3840

auuucuuuga cucccgccaa gcuccgaaga acaugccuca ggaugccgac gccaauggcg     3900

cuuaccacau ugggcugaaa ggacucaugc ugcugggccg gaucaagaac aaucaggaag     3960

ggaaaaagcu gaaccuugug aucaaaaaug aggaauauuu cgaguuuguu cagaacagaa     4020

auaacaagcg gcccgccgcg acaaagaagg cuggacaagc caagaaaaag aaauaaggac     4080

uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu     4140

cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa agaauu                                                     4216


<210>  4730
<211>  4530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4730
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccga caagaaauac      120

agcauuggac uggauaucgg caccaacucc guuggguggg cggugauuac agacgaguau      180

aaggugccgu cuaagaaauu caagguccuc ggaaauacgg aucggcauag caucaaaaag      240

aaccugauug gcgcucugcu guuugacucc ggggaaaccg ccgaggccac acgccucaaa      300

agaaccgcuc ggcgcagaua cacacggcgc aagaauagaa ucugcuaucu gcaggaaauc      360

uucucuaacg agauggccaa gguggaugac agcuuuuucc accggcuuga agaguccuuu      420

cugguugagg aagauaaaaa gcaugagcgc caccccauuu ucggaaauau cguggacgaa      480

gucgcuuacc augagaaaua uccuaccauu uaccaccuca gaaagaaacu gguggauucu      540

acagacaagg ccgaucugcg gcucaucuau cuggcgcuug cccacaugau uaaauuucgc      600

ggccauuucc ugaucgaggg cgaccucaac cccgauaaua gcgacguuga caagcuguuu      660

auucaacuug ugcagaccua caaccagcug uucgaagaga auccuaucaa cgcuuccggg      720

gucgaugcca aggcuauccu cagcgccaga cugucuaaau cccggcgccu ggaaaaucuc      780

auugcgcagc ugcccggaga gaagaaaaac ggccuuuuug ggaaucugau cgcccucagc      840

cugggacuua cgccuaacuu caagucuaau uuugaccugg cugaagaugc caaacuccaa      900

cuguccaagg acaccuacga ugacgaucug gacaaccucc uggcucagau uggcgaucag      960

uaugccgacc uuuuccuggc cgcuaagaac cucagcgaug cgauccugcu uucugacauu     1020

cugagaguga auacagagau caccaaagcc ccgcucuccg cuagcaugau caagcgguac     1080

gacgagcacc aucaggaucu gacacugcuc aaagcccugg ugagacaaca gcuucccgaa     1140

aaguauaaag agauuuucuu ugaccagucc aagaacgggu acgccggaua uaucgauggc     1200

ggcgcuucuc aggaagaguu cuacaaguuu auuaaaccua uccuggaaaa gauggacggg     1260

accgaggagc uccuggucaa acugaaucgc gaagaucuuc uccggaagca aagaacguuc     1320

gacaacggaa gcauucccca ccagauccac cugggcgagc ugcaugccau ccuccgccgg     1380

caggaagauu uuuauccuuu ccugaaagac aauagagaga agauugaaaa gauccuuaca     1440

uuucgcauuc ccuacuacgu ugggccgcug gcgcggggaa acuccagauu cgcuuggaug     1500

acccgcaaaa gcgaggagac aaucaccccu uggaauuuug aagagguggu ggauaagggc     1560

gccucugccc aauccuucau ugaacggaug accaacuuug acaaaaaucu ccccaacgag     1620

aagguccugc cuaaacacag ccugcuuuau gaguacuuca caguguauaa ugaacucacg     1680

aagguuaaau acgugaccga ggggaugaga aagcccgcuu uucugucugg agaacagaag     1740

aaagccaucg ucgaucuucu cuucaagaca aaccgcaaag ugaccguuaa gcagcugaaa     1800

gaggacuauu ucaagaagau ugaauguuuu gauagcgugg agaucuccgg cgucgaggac     1860

cgguucaaug cuucucuugg cacauaccau gaccugcuca aaaucauuaa ggauaaagac     1920

uuucuggaua acgaagagaa ugaagacauc cuggaggaua uugugcuuac ccucacgcug     1980

uucgaagaca gagagaugau cgaggaacgg cugaagacau augcccaccu cuuugaugac     2040

aaagucauga agcagcugaa gcgcagacgg uacaccgggu ggggaagacu uagccgcaaa     2100

cugauuaacg gcauccggga uaagcaaucc gggaaaacca uccucgacuu ccuuaagagc     2160

gauggauuug cgaacagaaa uuucaugcag cugauucaug acgauucucu gacauuuaaa     2220

gaggacaucc agaaggccca gguguccggc caaggggaca gccuccacga acacauugcu     2280

aaccuggccg gcucucccgc uaucaagaaa ggcauucugc agaccguuaa gguggucgau     2340

gagcucguga aaguuauggg acgccauaag ccugaaaaua ucgugaucga gauggccaga     2400

gagaaccaga caacgcagaa agggcaaaag aacucccggg aacgcaugaa aagaauugag     2460

gaaggaauca aggagcuggg cagccagauu cuuaaggagc acccggucga aaauacccag     2520

cugcagaaug agaaacucua ccuguauuac cugcaaaacg ggcgggacau guauguggau     2580

caggaacuug acaucaaucg ccuguccgau uacgacgugg aucauauugu uccccagucu     2640

uuccucaagg acgauagcau cgacaacaaa guccugacaa gauccgauaa gaaucgggga     2700

aaaucugaca acgugccuag cgaggaagug gucaagaaga ugaaaaauua uuggcgccaa     2760

cuccugaacg cgaaacuuau cacccagaga aaauuugaca aucugacaaa ggccgagcgg     2820

ggcgggcucu ccgagcugga uaaagcuggc uucauuaagc gccagcuggu ggaaaccaga     2880

cagaucacga agcacguugc ccaaauucuu gacagccgga ugaacaccaa auacgaugag     2940

aacgacaagc ugaucagaga agugaaaguc auuacacuca agucuaaacu gguguccgau     3000

uuucgcaagg acuuccaguu cuauaagguu cgggagauca auaacuacca ccaugcucac     3060

gaugccuacc ucaaugccgu ggucggaacc gcucugauua aaaaguaucc caaacuugaa     3120

agcgaguuug uguacggcga cuauaagguu uacgauguga gaaaaaugau cgcgaagucu     3180

gagcaggaaa ucgggaaggc cacagcuaaa uauuucuuuu acuccaacau uaugaauuuc     3240

uuuaagaccg agaucacgcu ggccaacgga gaaauucgca aacggccucu caucgagaca     3300

aauggcgaaa ccggggagau ugucugggac aagggaagag auuucgccac agugcgcaaa     3360

gugcugagca ugccccaggu caacaucguu aagaaaaccg aggugcaaac cggcggguuu     3420

ucuaaggaaa gcauccugcc gaagagaaau uccgacaaac uuauugcucg gaagaaagau     3480

ugggacccua agaaauaugg cggauucgac agccccacag uggccuacuc uguccuggug     3540

guugcgaagg uggagaaggg caaauccaag aaacucaaga gcgucaaaga acugcucggg     3600

aucacgauua uggagcgcuc cucuuuugag aagaacccua ucgauuuccu ggaagcuaag     3660

ggauacaaag aggugaagaa agaccuuauu aucaagcugc ccaaauauag ccuguuugaa     3720

cucgagaaug gccggaagag aaugcuggcc uccgccgggg aacuucagaa gggaaacgag     3780

cuggcucucc cuucuaaaua cguuaauuuc cuguaucucg ccagccauua cgagaagcug     3840

aaaggcuccc ccgaagauaa cgagcagaag cagcuuuucg uggaacaaca caaacauuau     3900

cuggacgaga ucauugaaca gaucucugag uuuagcaagc gggucauucu ggcggaugcu     3960

aaucucgaca aagugcuguc cgccuacaac aagcaccgcg auaagccgau cagagagcag     4020

gccgaaaaca uuauccaccu uuucacccug acaaaucucg gggcuccugc cgcuuuuaaa     4080

uauuucgaca ccacaauuga ucggaagcgc uacaccagca cgaaagaggu ucuggacgcc     4140

acacucaucc aucaaucuau caccggccug uacgaaacca gaauugaucu uucccagcug     4200

ggaggcgaca agcggcccgc ggccacaaag aaggcugggc aggccaagaa aaagaaauaa     4260

ggacuagugc aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau     4320

gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu     4380

cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa     4440

aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4530


<210>  4731
<211>  5313
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4731
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccaa cuucaagauu      120

cugccgaucg cgauugaccu cggaguuaaa aauacaggcg uguuuagcgc uuucuaccag      180

aaggggacau cccuggagcg gcuugauaac aagaauggaa aaguguauga acugucuaag      240

gacagcuaca cgcuccugau gaacaaucgc accgccagac ggcaucaacg cagaggcauc      300

gaucggaaac agcuugucaa gcgccuguuu aaacucauuu ggacagagca gcugaaccug      360

gaaugggaca aggauaccca gcaagccauc uccuuccucu uuaauagacg gggguucucu      420

uuuaucacag acggauauag ccccgaguac cugaacauug ugccugaaca gguuaaggcu      480

auccuuaugg auauuuucga cgauuauaau ggcgaggacg aucuggacuc cuaccucaaa      540

cuggccaccg agcaggaauc uaagaucagc gagauuuaua acaaacuuau gcagaagauc      600

cuggaauuua aacucaugaa gcugugcaca gauauuaaag acgacaaggu guccaccaag      660

acgcugaaag agaucaccag cuacgaguuc gaacuccugg cugauuaccu ugccaauuau      720

ucugaguccc ugaagacaca aaaauuuagc uacaccgaca agcagggcaa ccucaaagaa      780

cugucuuauu accaccauga uaaguauaau auccaggagu uccuuaagcg ccacgcgaca      840

auuaacgaca gaauccugga uacccuccug acggacgauc uggacauuug gaauuuuaac      900

uucgaaaaau ucgauuuuga caagaacgag gagaaacucc agaaucaaga agauaaggac      960

cauauccagg cccaucugca ccacuucguc uuugcuguga acaaaauuaa guccgagaug     1020

gccagcgggg gacggcaccg cucucaguac uuccaagaaa ucacaaaugu ucuugacgag     1080

aacaaucauc aggaaggcua ucugaagaac uuuugugaga aucuccacaa caaaaaguac     1140

uccaaucuga gcgugaaaaa ccuugucaau cugaucggga accucuccaa ucuggagcug     1200

aagccccuca gaaaauacuu caacgauaag auucacgcua aggccgacca uugggaugaa     1260

cagaaauuua ccgagacaua uugccacugg auccugggag aauggcgggu gggcgugaag     1320

gaccaggaua aaaaggacgg ggcgaaauac ucuuauaagg aucuuuguaa ugagcugaaa     1380

caaaagguca ccaaggccgg acucguugac uuccugcugg aacuugaucc uugccgcacc     1440

auucccccuu accucgacaa caacaauaga aaaccgccca agugucagag ccugauccug     1500

aacccuaaau uucucgauaa ucaguauccc aacuggcagc aauaccugca ggagcuuaag     1560

aaacugcagu ccauucagaa uuaucucgac agcuucgaga cagaucugaa ggugcugaag     1620

ucuuccaaag accaaccuua cuuuguggaa uacaagagcu cuaaccagca gaucgcuagc     1680

ggccagcggg acuauaaaga ucuugacgcc agaauccucc aauucauuuu ugaucgcguc     1740

aaggcuuccg acgagcugcu gcucaaugaa aucuacuucc aggccaaaaa gcugaagcag     1800

aaagccucua gcgagcuuga gaagcuggaa uccagcaaaa agcucgauga ggugauugcu     1860

aacucucaac ugucccagau ccugaaaagc cagcauacga auggcauuuu cgaacagggg     1920

accuuucuuc accucguuug caaguauuac aagcaacggc agagagcgcg cgacucucgg     1980

cuguauauca ugcccgagua cagauaugau aaaaagcugc auaaauacaa caauacagga     2040

cgcuucgacg augacaacca gcuccugacc uacuguaauc acaagccgcg gcagaaaaga     2100

uaucaacuuc ugaacgaucu cgccggcgug cugcaggucu ccccuaauuu ucugaaggac     2160

aaaauuggga gcgaugacga ccuuuucauc uccaaguggc ucguggaaca cauccgcgga     2220

uuuaagaagg cuugcgagga uucucugaaa auucagaaag acaaccgggg ccugcucaac     2280

cauaagauca auauugccag aaacacaaaa gggaagugug agaaggaaau cuucaaucug     2340

auuugcaaaa ucgagggaag cgaagauaag aaaggcaacu acaagcacgg ccuugccuau     2400

gagcuggggg uucuccuguu uggagaaccc aaugaggcuu ccaaaccuga guucgaccgc     2460

aagaucaaga aauuuaacuc uauuuacagc uucgcccaga uccaacagau ugcguuugcu     2520

gaacggaagg gcaaugccaa caccugugcc gugugcuccg cugauaaugc ccauagaaug     2580

cagcaaauca aaauuacgga gcccgucgaa gacaacaagg auaaaaucau ccugagcgcu     2640

aaggcccagc ggcuucccgc gauuccuaca cgcaucgugg acggggccgu caagaaaaug     2700

gcuaccauuc uggccaagaa uaucguggau gacaacuggc agaauauuaa acagguucuc     2760

ucugcuaagc accaacugca caucccgauc auuaccgagu ccaacgccuu cgaauuugag     2820

cccgcgcucg ccgaugugaa aggaaagagc cugaaggaca gacggaaaaa ggcucuugag     2880

agaaucucuc cugaaaauau uuucaaagac aagaacaacc gcaucaaaga guucgccaag     2940

ggcauuuccg cuuauagcgg ggccaaucug acagauggcg acuuugaugg agccaaagag     3000

gaacucgacc auaucauucc ccggucucac aagaaguacg gcacccugaa cgaugaggcu     3060

aaucugaucu gcgucacaag aggggacaac aaaaauaagg gaaaccgcau cuucugucuu     3120

agagaucugg cggacaauua uaaacucaag caguuugaaa cgaccgauga ccuggagauu     3180

gagaaaaaga ucgccgauac aauuugggac gcuaacaaga aagauuucaa guuuggcaau     3240

uaccggagcu ucaucaaccu caccccucag gaacagaaag ccuuucgcca ugcccuguuc     3300

cuugcugacg aaaaucccau uaagcaagcc gugaucagag cgaucaacaa ucggaaccgc     3360

acauuuguua augggaccca gagauacuuc gcugaggugc uggccaacaa uauuuaucuc     3420

cgggccaaaa aggagaaccu gaacacggac aagaucuccu uugauuacuu cggcauuccg     3480

accaucggaa augggcgcgg cauugcugaa aucagacagc uguaugagaa agucgacagc     3540

gauauccagg ccuacgcgaa gggagacaaa ccucaggcuu cuuauuccca ccuuauugau     3600

gccaugcugg ccuucugcau cgcugccgac gaacaucgga acgauggcag cauugggcuc     3660

gagaucgaca agaauuacuc ccuguauccc cucgauaaaa acacaggaga aguguuuacc     3720

aaggacauuu ucucucaaau caagauuaca gauaaugagu uuagcgacaa aaagcuggug     3780

cgcaaaaagg cuaucgaggg cuucaacacc cacagacaga ugacgcggga ugggaucuac     3840

gccgaaaauu accuuccuau ucugauccac aaagagcuga acgaaguuag aaagggauau     3900

acauggaaaa auuccgagga aauuaagauc uuuaagggca aaaaguacga cauucagcaa     3960

cucaacaauc uggucuauug ucuuaaauuc guggacaagc ccaucucuau cgauauucag     4020

aucagcaccc uggaggagcu ccgcaacauu cugacaacca auaacaucgc ggccaccgcu     4080

gaauacuauu acauuaaucu caaaacacag aagcugcaug aguauuacau cgaaaacuac     4140

aacacggccc uuggguauaa gaaauacucc aaggagaugg aguuucugcg gucucuggcu     4200

uauagaagcg aacgcgugaa aaucaagucc auugacgaug ucaaacaagu gcucgacaag     4260

gauagcaauu ucaucauugg caagaucacc cugccuuuua aaaaggagug gcagcggcuu     4320

uacagagaau ggcagaacac aaccauuaaa gacgauuaug aguuccugaa gucuuucuuc     4380

aauguuaaau ccaucacaaa gcuccacaag aaagugcgca aggacuuuag ccugcccauu     4440

ucuaccaacg aaggaaaauu ccucgucaag cggaaaacgu gggauaauaa cuucaucuac     4500

cagauccuga augacuccga uagcagagcc gacggcacaa agccguuuau uccugccuuc     4560

gacaucucua aaaacgagau uguggaggcu aucauugauu ccuuuaccag caagaauauc     4620

uucuggcuuc ccaagaacau cgaacugcaa aaaguugaca auaagaacau uuuugcgauc     4680

gauaccucca aaugguucga gguggaaaca cccagcgacc ugcgcgauau ugggaucgcc     4740

accauucagu auaagaucga caauaacucu cggccuaaag ucagagugaa gcucgauuac     4800

guuaucgacg augacagcaa gauuaauuac uuuaugaacc auucccugcu uaaaucucgg     4860

uauccggaua aggugcugga gauccucaaa cagagcacaa uuaucgaauu ugaguccucu     4920

ggauuuaaca agacgauuaa agagaugcug ggcaugaagc ucgcugggau cuacaaugaa     4980

accagcaaca auaagcgccc cgccgccacc aaaaaggcug gacaggccaa aaagaaaaag     5040

uaaggacuag ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa     5100

aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc     5160

ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau     5220

aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5280

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  5313


<210>  4732
<211>  3585
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4732
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccaa gcggaacuac      120

auucugggac ucgacaucgg cauuaccagc guuggguaug gaaucauuga uuacgagaca      180

cgcgacguga ucgaugcggg cgugagacug uucaaagaag cuaaugucga gaacaaugaa      240

gggcggcgcu ccaagagagg agcccggcgc cuuaagagac ggcgcagaca ucggauccag      300

cgcgugaaaa agcugcucuu ugacuauaac cugcuuacgg aucacucuga gcugagcggc      360

auuaauccgu acgaagccag aguuaaaggc cucucccaaa agcugucuga ggaggaguuc      420

agcgcugccc ugcuccaucu ggcuaaacgg cgcggggugc acaacgucaa ugaaguggaa      480

gaggacaccg gaaacgagcu uuccacaaag gaacagaucu cuagaaauag caaggcccug      540

gaggaaaaau auguugcgga gcuccagcug gaacggcuua agaaagaugg cgaggugaga      600

ggguccauua accgcuuuaa gaccagcgac uacgucaaag aggccaagca gcugcucaaa      660

gugcaaaagg cuuaucauca gcuggaucag ucuuucaucg acacauacau ugaucugcuc      720

gaaacccgga gaacauacua ugagggaccc ggcgaagggu ccccuuuugg auggaaggac      780

aucaaagagu gguacgaaau gcugaugggc cacugcaccu auuuccccga ggagcuucgc      840

agcgugaagu acgccuauaa ugcugaucug uacaacgccc ucaaugaccu gaacaaucuu      900

gucauuacgc gggacgaaaa cgagaaacug gaauauuacg agaaguuuca gaucaucgaa      960

aacguuuuca aacaaaagaa gaaaccuacc cucaagcaga uugcgaaaga gauccuggug     1020

aaugaggaag auauuaaggg cuacagagug acaucuaccg ggaaacccga guuuacaaac     1080

cugaaggucu aucacgacau caaggauauu accgcccgca aagaaaucau cgagaaugcu     1140

gagcuccugg accagauugc caagauccuu acgauuuacc aguccagcga agauauccaa     1200

gaggaacuga caaaccucaa uucugagcug acccaggaag agauugagca gaucuccaac     1260

cuuaaaggau auacaggcac ccauaaucug agccucaagg cuaucaaccu gauucuggac     1320

gaacucuggc acaccaauga uaaccaaauc gccauuuuca aucggcugaa acuugugccu     1380

aagaagguug accuguccca gcagaaagag aucccgacaa cgcucgugga ugacuuuauu     1440

cugucucccg ucgugaagcg cagcuucauc caguccauua aaguuaucaa cgccaucauu     1500

aagaaauacg ggcugccuaa ugauaucauu aucgaacuug cucgcgagaa gaacagcaaa     1560

gacgcgcaaa agaugauuaa ugaaaugcag aagcggaaca gacagaccaa cgagcgcauc     1620

gaggaaauca uucggacaac cggaaaagag aaugccaagu aucucaucga gaaaauuaag     1680

cugcaugaca ugcaggaagg caaaugucug uacucucucg aagcuauccc ccuggaggau     1740

cuucugaaca auccuuuuaa cuaugaggug gaccacauua uccccagauc cgucagcuuc     1800

gauaauucuu ucaacaauaa ggugcucguc aagcaagaag agaacagcaa aaaggggaau     1860

cggacaccgu uucaguaccu guccucuagc gacuccaaaa ucagcuacga aaccuucaag     1920

aaacacauuc ugaaccuugc caagggaaag ggccgcaucu cuaaaacgaa gaaagaguau     1980

cuccuggagg aaagagauau uaaucgguuu uccgugcaga aggacuucau caacagaaau     2040

cugguugaua cacgcuacgc cacccggggc cucaugaacc ugcuuagaag cuauuuucgc     2100

gugaauaacc uggacgucaa agugaagucu auuaacgggg gauucaccuc cuuucucaga     2160

cggaagugga aauucaagaa agagcgcaau aagggcuaca aacaucacgc ugaagaugcc     2220

cugaucaucg cgaacgcuga cuuuauuuuc aaggagugga aaaagcugga uaaggccaaa     2280

aagguuaugg aaaaucagau guuugaggag aaacaagccg aaagcaugcc ugagaucgaa     2340

acagagcagg aauauaagga gauuuucauc acaccccauc agauuaaaca caucaaggac     2400

uuuaaggauu acaaauauuc ccauagagug gacaagaaac cuaaccggga gcuuauuaau     2460

gacacccucu acucuacgcg caaggaugac aaagggaaca cccugaucgu caauaaccug     2520

aauggacucu acgauaagga caacgauaag cugaagaaac uuaucaauaa aagccccgaa     2580

aagcugcuca uguaucacca cgacccccag acauaccaaa aacugaagcu gauuauggag     2640

caguauggcg augaaaagaa cccucuuuac aaauauuacg aggaaaccgg gaauuaucuc     2700

acaaaguacu ccaaaaagga caacggcccg gugaucaaaa agauuaagua cuauggaaau     2760

aaacugaacg cucaucugga uaucaccgac gauuacccca acucuagaaa uaaggugguu     2820

aaacucagcc ugaagccuua ucgguucgac gucuaccuug auaacggcgu guauaaauuc     2880

gugacgguca agaaucugga cgugauuaaa aaggagaacu acuaugaggu uaauuccaag     2940

ugcuacgaag aggccaaaaa gcucaaaaag aucagcaacc aggcugaauu uaucgccucu     3000

uucuacaaua acgaccugau uaaaaucaau ggggagcugu aucgcgugau uggagucaac     3060

aaugaucuuc ugaacagaau cgaggugaau augauugaca ucaccuaccg ggaauaucuc     3120

gagaacauga augauaagag acccccuaga aucauuaaga caaucgcguc caaaacccaa     3180

agcauuaaga aauacucuac agacauccug ggcaaccucu augaaguuaa guccaaaaag     3240

cacccccaga uuaucaagaa agggaagcgg ccggccgcua ccaaaaaggc cggacaggcu     3300

aaaaagaaga aauaaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa     3360

uaagagaaag aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua     3420

aagccaacac ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu     3480

gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3585


<210>  4733
<211>  4653
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4733
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugcccu guucaacaag      120

ugcauuauca uuagcaucaa ucucgacuuu uccaacaaag agaaguguau gaccaagccg      180

uacucuauug gacuggauau cggcacaaau agcguugggu gggcggugau cacggacaac      240

uauaaagugc ccuccaagaa aaugaagguc cuuggaaaua ccucuaaaaa guacauuaag      300

aaaaaccugc ucggcgugcu gcuuuucgau agcgggauca cagcugaagg acggcgccug      360

aagagaaccg cccggcgcag auauacacgg cgcagaaauc ggauucucua ccugcaggag      420

aucuuuucca ccgaaauggc cacacuggac gaugcuuucu uucaacgccu cgacgauucu      480

uuccugguuc cugacgauaa aagagacagc aaguauccca uuuuuggcaa ccuuguggag      540

gaaaaagucu accaugauga guucccuacc aucuaucauc ugcggaagua ccucgccgac      600

uccacgaaaa aggcugaucu gcgccuugug uaccuggccc ucgcgcacau gauuaaguau      660

agaggccacu uucugaucga gggggaguuc aauagcaaaa acaaugacau ccagaagaac      720

uuucaggacu uccuggauac cuacaaugcc auuuuugaau cugaccucuc ccuggaaaac      780

agcaaacagc uugaggagau cguuaaggau aaaauuucua agcuggaaaa gaaagaccgg      840

auccucaagc uguuccccgg agagaaaaau uccggcauuu ucagcgaauu ucuuaagcug      900

aucgugggga accaagcuga uuuccgcaaa ugcuuuaacc ucgacgagaa ggccucucug      960

cauuucucca aggaaagcua ugaugaggac cuggagacac uccugggaua caucggcgau     1020

gacuauuccg augucuuucu uaaagcuaag aaacuguacg acgccauucu ccugucuggg     1080

uuccuuaccg ugacagacaa ugaaaccgag gcgccucuga gcuccgccau gaucaagaga     1140

uauaacgaac acaaagagga ucucgcucug cugaaggaau acauucggaa uaucagccuc     1200

aagacguaca acgagguguu uaaagacgau acaaagaaug gauaugccgg cuacauugac     1260

ggcaaaacca accaggagga uuucuauguc uaccugaaga aucuucuggc ugaauuugag     1320

ggggccgacu auuuccucga aaagaucgau agagaggacu uucugcgcaa gcagcggaca     1380

uucgauaacg gaucuauccc guaccagauu caucugcaag aaaugagagc cauccuugac     1440

aaacaggcua aguuuuaucc cuuccucgcg aagaauaaag agcgcauuga gaagauccug     1500

accuuccgga uuccuuacua cguuggcccc cuggccagag ggaacuccga uuuugcuugg     1560

agcauccgca aacggaauga aaagauuacc ccuuggaacu ucgaggacgu gaucgauaaa     1620

gaaucuagcg ccgaggccuu uaucaauaga augacauccu ucgaccucua ucugcccgag     1680

gaaaaggugc uuccgaagca cucucugcuc uacgagacgu uuaacgucua uaaugaacug     1740

accaaagugc gcuucauugc ugagagcaug cgggacuacc aguuucugga uuccaagcag     1800

aaaaaggaca ucguuagacu uuauuucaaa gauaagcgca aggugacaga caaagauauu     1860

aucgaauacc uccacgccau uuauggauac gacggcaucg agcugaaggg gaucgagaaa     1920

caauuuaaca gcucucuguc caccuaccau gaucuccuga acauuaucaa ugacaaggag     1980

uuccuugaug acagcucuaa cgaagcgauu aucgaagaga uuauccacac acugaccauc     2040

uuugaagauc gggagaugau uaaacagaga cucuccaagu ucgagaauau cuuugacaag     2100

agcguccuga aaaagcuguc ccggcgccac uauacgggau ggggcaaacu uucugcuaag     2160

cucauuaacg gcaucagaga ugaaaagagc gggaauacaa uucuggacua ccugaucgac     2220

gauggaaucu ccaaccggaa uuucaugcag cucauucaug acgaugcccu gucuuucaag     2280

aaaaagaucc aaaaggccca gauuaucggc gacgaggaua aagggaacau uaaggaagug     2340

guuaaaagcc uuccuggauc ccccgcuauc aagaaaggca uucugcagag caucaagauc     2400

guggacgagc ucgucaaggu gaugggcggg agaaaaccug aaucuauugu cguggagaug     2460

gcccgcgaga aucaguauac caaccaagga aaguccaaua gccagcagcg gcugaaaaga     2520

cuggaaaagu cucuuaaaga gcucggcucc aagauccuga aggaaaacau ucccgcuaaa     2580

cugagcaaga ucgauaauaa cgcccuccag aaugaccgcc uguaccuuua uuaccugcaa     2640

aacgggaaag auauguauac cggagacgau cucgacauug acagacuguc uaacuacgau     2700

aucgaccaca ucauucccca ggcguuucug aaggauaaua gcaucgacaa caaaguucuu     2760

guguccagcg ccucuaaucg gggcaagucc gaugacuucc cuagccugga ggucgugaag     2820

aaacgcaaga cauuuuggua ccagcuccug aaauccaagc ucauuucuca gagaaaauuc     2880

gauaaccuga ccaaggcuga gcgcggggga cuucugccgg aagacaaggc cggcuuuauc     2940

caacggcagc ucguugagac aagacagauu acgaaacaug uggcucggcu gcuggaugaa     3000

aaguucaaua acaaaaagga cgagaauaac cgcgccguca gaaccgugaa aaucaucaca     3060

cuuaagagca cccugguguc ccaguuucgg aaagauuucg aacucuauaa gguucgcgag     3120

auuaaugacu uucaccaugc gcacgaugcc uaccugaacg cugucaucgc cucugcucuc     3180

cugaagaaau aucccaaacu ugagccugag uucguguacg gggacuaucc caaauacaau     3240

agcuuuagag aacggaaguc cgccacagaa aaaguguauu ucuacucuaa cauuaugaau     3300

aucuucaaaa agagcauuuc ccuggccgac ggcagaguca ucgagcgccc ucucauugaa     3360

gugaacgagg agaccggaga aagcguuugg aauaaagagu cugaucuggc uacggugcgg     3420

agaguccugu ccuaccccca agugaacguu gugaagaaag ucgaggaaca gaaccacggc     3480

cuugaccgcg ggaagccgaa aggacuguuu aaugcgaacc ucagcucuaa gccuaagccc     3540

aauuccaacg aaaaucuggu gggcgccaaa gaguaucucg auccuaagaa auacggggga     3600

uaugcuggca ucagcaacuc uuucgccguu cuggugaagg ggaccaucga gaaaggcgcc     3660

aagaagaaaa uuacaaaugu ccuugaauuu cagggaaucu ccauucugga ccggaucaac     3720

uacagaaagg auaaacugaa uuuccuccug gagaagggcu auaaagacau ugaacuuauc     3780

aucgagcugc ccaaguacag ccucuuugag cuguccgaug ggagccgccg gaugcucgcu     3840

ucuauucuga gcaccaacaa uaaaagagga gaaauccaua agggcaacca aauuuuccuu     3900

ucccagaagu uugugaaacu gcuguaucac gccaagcgca ucucuaauac aauuaacgag     3960

aaucaucgga aauacgugga aaaccacaag aaagaguucg aggaacucuu uuacuauauc     4020

cuggaguuca augaaaacua cgucggggcg aagaagaacg gaaaacuucu gaauagcgcu     4080

uuucaguccu ggcagaacca cucuaucgac gagcucugua gcuccuucau uggcccuacc     4140

gggucugaaa gaaagggccu guuugagcuc acgagccggg gauccgccgc cgauuucgag     4200

uuccugggcg uuaaaauccc ccgcuauaga gacuacacac cgagcucucu ucugaaggau     4260

gcuacccuga uucaucaauc cgugacaggg cucuaugaaa cccggaucga ccuggccaaa     4320

cuuggagagg gcaagcgccc ugcggcuacc aagaaagccg ggcaggccaa gaaaaagaaa     4380

uaaggacuag ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa     4440

aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc     4500

ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau     4560

aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu                                  4653


<210>  4734
<211>  3729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4734
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccug guacgcgagc      120

cugauguccg cucaucaccu ccggguugga auugacgugg gcacccauuc uguggggcug      180

gccacacuuc gcgucgauga ccacggaacg ccgaucgagc ugcucagcgc ccugucccau      240

auucacgauu cuggcguggg gaaggaaggg aaaaaggacc acgauaccag aaagaaacuu      300

agcggcaucg cucggcgcgc cagacggcug cuccaucacc gcagaacaca gcugcaacag      360

cuggacgagg uucuccggga ucugggcuuc cccauuccua cccccgggga auuucuugac      420

cugaacgagc agacagaucc uuaucgcgug uggagagucc gggcucgccu cguggaagag      480

aagcugcccg aggaacuuag aggaccugcc aucuccaugg cgguucggca uaucgcccgc      540

cacagaggcu ggcggaaucc guacucuaaa guggagagcc ugcucucccc cgcugaagag      600

agcccuuuca ugaaggcccu gagagagcgc auucuggcua ccacagggga aguccucgac      660

gauggaauca cccccggcca ggccauggcg caaguggccc ugacgcacaa cauuucuaug      720

cgggggccug agggaauccu uggcaaacug caucaguccg acaaugcuaa cgaaauuaga      780

aagaucugcg cccgccaggg cgugagcccc gaugucugua agcagcuccu gcgggcuguu      840

uuuaaagccg acucuccgag aggguccgcc gugagccgcg uggcuccuga cccccuuccu      900

ggacaaggcu cuuuccggag agcgcccaag ugcgaucccg aguuucagcg cuuccggauu      960

aucuccaucg ucgccaaucu gagaauuagc gaaaccaaag gggagaaccg cccucucaca     1020

gcugacgagc ggagacacgu gguuaccuuu cugacagaag auucccaggc cgaccugacc     1080

uggguggaug ucgccgagaa gcucggagug caucggcgcg accugagagg cacggcuguu     1140

cacacagaug acggggaacg gucugccgcg agaccgccca ucgaugcuac cgaccgcauu     1200

augcggcaga caaaaaucag cucccuuaag accugguggg aggaagccga uagcgagcaa     1260

agaggagcca ugauucgcua ucuguacgag gacccuaccg acucugaaug ugcugagauc     1320

aucgccgaac uccccgagga agaucaggcu aaacuggacu cccuucaucu gccugccggc     1380

agagcggccu auagccggga gucucucaca gcucugagcg aucacaugcu ggccacgacc     1440

gacgaucucc acgaggcucg caagagacug uucggcgugg acgauuccug ggcgcccccg     1500

gccgaagcca uuaaugcucc ugucgggaac cccucugugg accggacacu uaagaucguc     1560

ggacgcuacc ugagcgccgu ggaguccaug uggggcaccc cugaaguuau ucauguggag     1620

cacgucagag auggguuuac aagcgagcgg auggcugacg aacgcgauaa agccaauaga     1680

cggcgcuaua acgacaauca ggaggccaug aagaaaaucc aaagagauua cggaaaggaa     1740

ggcuacauuu cucggggaga caucgugaga cucgacgcuc uggagcugca gggcugcgcg     1800

ugucuuuauu gcggaaccac gaucggcuac cauacauguc agcucgauca cauuguuccc     1860

caggccgggc cuggauccaa caaucgccgg ggcaaccugg uggcugucug cgaaagaugu     1920

aaucgcagca aaucuaacac ccccuucgcc gugugggccc aaaagugcgg gaucccgcac     1980

gugggaguua aggaggcuau uggccggguc agaggguggc gcaaacagac cccuaacaca     2040

uccagcgagg accugacccg gcucaagaaa gaagugaucg ccagacugcg ccggacacag     2100

gaggaucccg aaauugacga gagauccaug gaaucugugg cguggauggc uaaugagcuu     2160

caucaccgga ucgccgccgc uuaucccgag acgaccguca ugguguaccg cggcagcauu     2220

acagccgcgg cuagaaaggc cgccggaauc gauucccgga ucaaccugau uggcgaaaaa     2280

gggcgcaagg acagaaucga ucggagacau cacgcuguug acgccucugu ggucgcucuc     2340

auggaggcca gcguggcgaa gacccuggcc gaacgcucca gccugcgggg agagcagaga     2400

cuuacaggca aagaacaaac cuggaagcag uauacggggu cuaccguugg agcucgcgag     2460

cauuuugaga uguggcgggg ccacaugcuc caccugacag aacuguucaa ugagagacuc     2520

gccgaagaua aaguguacgu cacccagaac auucgccugc ggcuuuccga cgggaaugcu     2580

cauacaguga acccuagcaa gcugguuucu cacagacucg gcgauggacu gaccgugcag     2640

caaaucgacc gcgccuguac gccggcccug uggugcgcuc uuacaagaga gaaagauuuu     2700

gacgagaaga auggccuccc cgcgcgggaa gaccgcgcca uuagagucca ugggcacgag     2760

aucaagucca gcgauuauau ccagguguuc ucuaaacgga agaaaaccga cagcgaucgc     2820

gacgaaacac cuuucggagc uauugccgug agaggcgggu uugucgagau cggacccucc     2880

auucaucacg cccggaucua cagaguugaa ggcaagaaac cuguguacgc uaugcugcgc     2940

guguucaccc acgaucugcu cagccagcgg cauggggacc uguuuucugc cgucauuccc     3000

ccucagucca ucagcaugag augcgcggag cccaaacuuc gcaaagcuau caccacaggc     3060

aacgccacgu aucugggaug ggugguugug ggcgaugagc ucgaaauuaa ugucgacucc     3120

uucaccaagu acgccaucgg gcgguuucug gaggauuucc cgaacacaac cagauggcgg     3180

auuuguggau augacacaaa uucuaagcug acccuuaaac cuaucgugcu cgcugccgaa     3240

ggccuggaga accccagcuc cgcgguuaau gaaauugugg agcugaaggg guggcgcguc     3300

gcuaucaacg ugcucacgaa aguucacccu acagugguca gacgggaugc ccugggacgc     3360

cccagauacu cuagccgguc caaucuuccg accucuugga ccaucgagaa gcgcccugcc     3420

gcuacaaaga aggccggcca agcuaagaaa aagaaauaag gacuagugca ucacauuuaa     3480

aagcaucuca gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau     3540

cucuuuuucu uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu     3600

aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaagaauu                                                             3729


<210>  4735
<211>  4590
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4735
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccac caagccguac      120

agcauuggac uggacaucgg cacaaacucc guuggguggg cggugacgac cgauaauuau      180

aaagugcccu cuaagaagau gaaaguccuc ggaaacacaa gcaagaaaua cauuaagaaa      240

aaucugcuug gcgugcuccu guucgacucc gggaucaccg cugagggacg gcgccugaag      300

agaacagccc ggcgcagaua uacccggcgc agaaaccgga uucuuuaccu gcaggaaauc      360

uuuucuacag agauggccac ccucgaugac gcuuucuuuc aacgccugga ugacagcuuc      420

cugguuccug augacaagag agauuccaaa uaucccaucu uuggcaaucu cguggaagag      480

aaggccuacc augacgaguu cccuacgauu uaucaucugc ggaaauaccu ugcugauucu      540

accaagaaag ccgaccugcg ccucgucuac cuggcgcuug cccacaugau caaguauaga      600

ggccacuuuc ugauugaggg ggaguucaac agcaaaaaua acgauaucca gaagaauuuu      660

caggacuucc ucgacacaua caacgcuauu uuugaauccg aucugagccu ggaaaauucu      720

aagcagcucg aagagaucgu gaaagacaag auuuccaaac uggagaagaa agaucggauc      780

cuuaagcugu uccccggaga aaagaacagc ggcaucuuuu cugaguuccu caaacugauu      840

guugggaauc aagccgacuu ccgcaagugc uuuaaccuug augaaaaggc uucccugcau      900

uucagcaaag agucuuauga cgaagaucuc gagacccugc ugggauacau cggcgacgau      960

uauuccgacg uguuucucaa agccaagaag cuguacgaug cgauucuucu gagcggguuc     1020

cucacaguca ccgacaauga gacggaagcc ccucuguccu cugcuaugau caaaagauau     1080

aacgagcaca aggaagaccu ugcccugcuc aaagaguaca uucggaacau cagccugaag     1140

acauacaaug aaguguuuaa agaugacacc aagaacggau augcuggcua caucgauggc     1200

aagacaaauc aggaggacuu cuauguguac cugaaaaagc uccuggccga guuugaaggg     1260

gccgauuauu uccuugagaa aauugacaga gaagauuuuc ugcgcaagca gcggaccuuc     1320

gacaacggau ccaucccgua ccagauucac cuccaagaga ugagagcuau ccuggauaaa     1380

caggcgaagu uuuaucccuu ccuggccaaa aauaaggaac gcauugagaa gauccuuacc     1440

uuucggaucc cuuacuacgu cggcccccuc gcuagaggga acagcgacuu cgccuggucu     1500

auucgcaaac ggaaugagaa gaucacaccu uggaacuucg aagauguuau ugacaaagag     1560

uccagcgccg aggcuuuuau caauagaaug acgucuuucg aucuguaucu gcccgaagag     1620

aaggugcucc cgaaacauag ccugcuuuac gaaaccuuua acguguauaa ugagcugaca     1680

aagguccgcu ucauugccga auccaugcgg gacuaccagu uucucgacuc uaagcagaaa     1740

aaggauaucg ugagacugua uuucaaagac aagcgcaaag uuaccgauaa ggacauuauc     1800

gaguaccugc acgcgaucua uggauacgau ggcauugaac uuaaggggau cgagaaacaa     1860

uuuaacagcu cccucagcac auaccaugac cugcugaaua uuaucaacga uaaggaguuc     1920

cucgacgauu cuuccaauga agcuauuauc gaggaaauca uucacacccu gacgaucuuu     1980

gaggaccggg aaaugauuaa acagagacuu agcaaguucg agaacaucuu ugauaaaucu     2040

gugcugaaga agcucucccg gcgccacuau acaggauggg gcaaacugag cgccaagcug     2100

auuaacggca ucagagacga gaaauccggg aauaccaucc uugauuaccu cauugacgac     2160

ggaaucucua accggaauuu caugcagcug auucaugaug acgcccugag cuuuaagaaa     2220

aagauccaaa aagcucagau uaucggcgau gaagacaagg ggaacaucaa ggaggucgug     2280

aaaucccucc cuggaucucc cgccauuaag aaaggcaucc ugcagagcau uaagaucguu     2340

gaugaacuug ugaaagucau gggcgggaga aagccugagu ccauuguggu cgaaauggcu     2400

cgcgagaauc aguauaccaa ccaaggaaag agcaauucuc agcagcggcu gaaaagacuc     2460

gagaaguccc ugaaagaacu gggcagcaag auccuuaaag agaacauucc cgccaagcuc     2520

ucuaagaucg acaauaacgc gcugcagaau gaucgccugu accucuauua ccugcaaaac     2580

gggaaagaca uguauacagg agaugaccuu gauaucgaca gacuguccaa uuacgacauu     2640

gaucacauca uuccccaggc cuuccucaag gacaacagca ucgauaauaa agugcugguu     2700

ucuagcgcuu ccaaccgggg caagagcgac gaugugccuu cucuggaagu cgugaaaaag     2760

cgcaagaccu ucugguacca gcuucugaaa uccaagcuca uuagccagag aaaauuugac     2820

aaccugacaa aggccgagcg cgggggacuc uccccggagg auaaagcugg cuucauccaa     2880

cggcagcugg uugaaacgag acagaucacc aagcaugugg cccggcuucu ggacgagaag     2940

uuuaauaaca aaaaggauga aaauaaccgc gcggucagaa cagugaaaau uaucacccuc     3000

aagucuacac uggugagcca guuccggaaa gacuuugagc uguauaaggu ucgcgaaauu     3060

aaugauuucc accaugccca cgacgcuuac cuuaacgccg ucguggcuuc cgcccugcuc     3120

aaaaaguauc ccaagcugga gccugaguuu guguacgggg auuaucccaa auacaauucu     3180

uucagagaaa gaaagagcgc caccgagaaa gucuauuuuu acuccaacau caugaauauu     3240

uucaagaaau cuaucagccu cgcugacggc cgggugaucg aacgcccucu gauugagguu     3300

aacgaagaga cgggagaguc cguguggaau aaggaaagcg accuugcgac cguccggaga     3360

gugcugucuu acccccaagu uaacgugguc aagaaagugg aggaacagaa ucacggccuc     3420

gaucgcggga agccgaaagg acuguuuaac gccaaucugu ccagcaagcc uaaacccaac     3480

ucuaacgaga aucuuguugg cgcuaaggaa uaucuggacc cuaagaaaua cgggggauau     3540

gccggcaucu ccaacagcuu cacagugcuc gucaagggga ccauugagaa aggcgccaag     3600

aaaaagauca caaaugugcu ggaguuccag ggaauuucua uccucgaucg gauuaacuac     3660

agaaaggaca aacugaauuu ucuucuggaa aagggcuaua aagauaucga gcugaucauu     3720

gaacucccca aguacucccu guucgagcuu agcgacgggu cccgccggau gcuggcuagc     3780

auccucucua ccaacaauaa aagaggagag auucauaagg gcaaccaaau cuuucugagc     3840

cagaaauucg ugaagcuccu guaucacgcc aagcgcauuu ccaauacgau caacgaaaau     3900

caucggaaau acgucgagaa ccacaagaaa gaauuugagg aacuuuucua cuauauccug     3960

gaguuuaaug agaacuacgu uggggcgaag aaaaauggaa agcugcucaa cucugcuuuc     4020

cagagcuggc agaaucacuc cauugaugaa cuguguucua gcuuuaucgg cccuacaggg     4080

uccgagagaa agggccuuuu cgaacugacc ucucggggaa gcgccgccga cuuugaguuc     4140

cucggcguga aaauuccccg cuauagagau uacacaccgu ccagccugcu caaggacgcu     4200

acccugaucc aucaaucugu gaccgggcuu uaugaaacac ggauugaucu ggccaaacug     4260

ggagagggca agcgcccugc ggcuacgaaa aaggccgggc aggccaagaa aaagaaauaa     4320

ggacuagugc aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau     4380

gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu     4440

cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa     4500

aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4590


<210>  4736
<211>  3672
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4736
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccgc ggcuuucaag      120

ccgaacagca uuaauuacau ccugggacuc gacauuggca ucgccuccgu ugggugggcc      180

augguggaga uugaugaaga ggaaaacccc auccggcuga ucgaccuugg agugcgcguc      240

uuugagagag cugaagugcc uaaaaccggc gauucucugg ccauggcucg gcgccucgcc      300

agaagcguuc ggcgccugac aagacggcgc gcgcauagac uucugcggac gcgcagacuc      360

cugaagcggg agggggugcu gcaggccgcu aauuucgacg agaacggacu cauuaagucc      420

cugcccaaua ccccuuggca acuucgcgcc gcugcccugg auagaaaacu cacaccccug      480

gaauggucug cgguccuucu gcaccucauc aagcaucggg gcuaucugag ccagcgcaaa      540

aacgagggcg aaaccgccga caaggagcug ggggcucucc ugaaaggagu ggccggcaau      600

gcucacgccc uucagacagg ggauuuuaga accccugccg agcuggcucu caacaaguuc      660

gaaaaggagu ccggacauau ucggaaucag agaucugacu acagccacac auuuucccgc      720

aaagaucugc aagcggaacu uauccuccug uucgagaagc agaaagaauu uggcaacccg      780

cacguuagcg ggggacugaa ggagggcauu gagacccugc ucaugacgca gcggcccgcc      840

cugucuggcg acgcugugca gaaaaugcuu gggcauugca ccuucgaacc ugccgagccc      900

aaggccgcua aaaauacaua uaccgccgaa agauuuaucu ggcugacaaa gcucaacaau      960

cugcgcauuc uugagcaagg auccgaacgg ccucugaccg auacggagag agcgacacuc     1020

auggacgagc ccuaccgcaa gagcaaacug accuaugcuc aggcccggaa gcugcucggc     1080

cuggaagaua cagccuucuu uaaagggcuu agauacggaa aggacaacgc ugaggccucu     1140

acccugaugg aaaugaaggc uuaucacgcc aucucccgcg cgcucgagaa ggaaggccug     1200

aaggacaaaa agagcccgcu gaaucuuucu ccugagcucc aggaugagau cgggaccgcc     1260

uucucccugu uuaaaacaga cgaagauauu acgggacggc ugaaggacag aauccagccc     1320

gagauucucg aagcucugcu uaaacauauc agcuucgaua aguucgucca aauuucccug     1380

aaggcccucc ggcgcaucgu gccucugaug gagcagggca aaagauacga cgaggcuugu     1440

gccgaaaucu acggcgauca cuaugggaag aaaaacaccg aggaaaagau uuaccugccu     1500

cccauccccg cggacgagau ucggaacccg gugguccuua gagcccucuc ucaggcucgc     1560

aaaguuauca auggaguggu gcggagauau ggcagccccg cccgcauuca caucgaaaca     1620

gcuagagagg ucgggaaguc cuuuaaggau cggaaagaga ucgaaaagcg ccaagaggaa     1680

aacagaaaag accgggagaa ggccgccgcu aaauuccgcg aauacuuucc uaauuucgug     1740

ggagagccca agagcaaaga uauucugaag cugagacucu augagcagca gcauggcaag     1800

ugccuguacu cugggaaaga aaucaaccuu ggccggcuga augagaaggg auauguugaa     1860

auugaccacg cgcucccuuu uucccgcacc ugggacgaua gcuucaacaa uaaagugcug     1920

guccugggcu cugagaacca gaauaagggg aaccaaacac ccuacgaaua cuuuaaugga     1980

aaagacaaca gcagagagug gcaggaguuc aaggcccggg uggaaaccuc ccgcuuuccg     2040

agaucuaaga aacagcggau ccuucuccag aaguucgaug aggacggcuu uaaagaaaga     2100

aaucugaacg auacgcgcua uguuaaucgg uuccuguguc aauuuguggc ugacagaaug     2160

cgccucacag ggaagggcaa aaagcggguc uucgccagca acggacagau uaccaaccug     2220

cuuagagggu ucuggggccu gcgcaaggug cgggccgaga augauagaca ucacgcucuc     2280

gacgccgucg ugguugcgug cuccaccgug gcuaugcagc agaaaaucac acgcuuuguc     2340

cgguacaagg agaugaacgc cuucgaugga aaaaccauug acaaggaaac aggcgaggug     2400

cugcaucaga aaacgcacuu uccucagccc ugggaguucu uugcccagga aguuaugauc     2460

agaguguucg ggaagccuga uggaaagccc gaauuugagg aggcugacac ccuggaaaaa     2520

cuucggacac uccuggccga gaagcugagc ucucgcccug aagcggucca cgaguaugug     2580

acaccccucu ucguguccag agcuccgaau cggaaaauga gcggccaggg gcauauggaa     2640

accguuaagu cugccaaacg ccuggaugag ggagucuccg ugcuuagagu gccucugacc     2700

caacucaagc ugaaagaccu ggagaagaug gucaaccggg aaagagagcc caaacuuuac     2760

gaagcccuga aagcucgccu cgaggcccac aaggacgauc ccgcuaaagc cuuugcggaa     2820

ccuuucuaua aguacgacaa agccggcaau cggacgcaac aggugaaggc uguuagagug     2880

gagcaagucc agaagaccgg gguguggguu cgcaaccaua auggcaucgc cgauaacgcu     2940

acaauggugc gggucgacgu guuugagaaa ggagauaagu auuaccuggu uccgauuuac     3000

agcuggcaag uggccaaagg cauccucccc gacagagccg ucgugcaggg gaaggaugaa     3060

gaggacuggc agcugauuga ugacuccuuc aauuuuaaau ucucucuuca cccuaacgau     3120

cugguggaag ucaucaccaa gaaggcucgc auguucggau auuuugcgag cugucaccgg     3180

ggcacaggga auauuaacau cagaauccau gaccucgauc acaaaauugg aaagaauggc     3240

auccuggagg ggauuggcgu uaaaaccgcc cuguccuucc agaaguacca gaucgacgag     3300

cuuggaaaag aaauucgccc cugcagacug aagaagcggc cucccgugcg caaaagaccu     3360

gcugccacga agaaagccgg ccaggcuaag aaaaagaagu aaggacuagu gcaucacauu     3420

uaaaagcauc ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu     3480

caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc     3540

uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga     3600

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaagaa uu                                                         3672


<210>  4737
<211>  4428
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4737
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccaa gaaaccguac      120

accauuggac uggacaucgg cacaaacagc guuggguggg cggugcucac ggaucaguau      180

gaccugguga agcggaagau gaaaauugcu ggagauuccg agaagaaaca aaucaagaaa      240

aauuucuggg gcguccgccu uuuugacgaa gggcagaccg ccgccgauag acggauggcu      300

cgcacagcca gacggcgcau ugagagacgg cgcaacagaa ucucuuaccu gcagggaauc      360

uucgcugaag agaugagcaa gaccgacgcc aauuucuucu gccggcucuc cgauucuuuu      420

uauguggaca acgaaaagcg caauagcaga caucccuucu uugcgacaau ugaggaggaa      480

guugaguacc acaaaaacua uccuaccauc uaccaucugc gggaggaacu ugucaauucc      540

ucugagaagg ccgaucugcg ccucgucuau cuggcucugg cccacauuau caaauacaga      600

ggcaacuucc ucauugaagg cgcucuggac acacagaaua ccagcgugga ugggaucuac      660

aagcaauuua uucagacgua uaaccagguu uucgccuccg gaaucgagga cggcagccuu      720

aaaaagcugg aagauaauaa agacguggcg aagauccucg ucgagaaggu gacccggaaa      780

gaaaagcugg agcgcauucu uaaacuguac cccggggaga agucugccgg aauguuugcu      840

caguucaucu cccucauugu gggcagcaaa gggaacuuuc aaaagccuuu cgaccugauc      900

gaaaagucug auauugagug ugccaaagac uccuaugaag aggaucugga aagccuccug      960

gcucuuaucg gagacgagua cgccgagcug uucgucgccg cuaagaaugc guauucugcc     1020

guugugcucu ccagcaucau uacaguggcu gaaaccgaga caaacgccaa acuguccgcc     1080

ucuaugaucg aaagauuuga uacccaugag gaagaccuug gcgagcugaa ggcuuucauu     1140

aaacuccauc ugcccaagca cuacgaggaa aucuuuagca auacggagaa gcacggcuau     1200

gccggguaca uugauggaaa aacaaagcag gcggacuucu acaaauauau gaagaugacc     1260

cuggaaaaca ucgagggcgc ugauuacuuu aucgccaaaa uugagaagga aaacuuccuc     1320

cggaagcaga gaacauuuga caauggggcc aucccucacc agcugcaucu ugaggaacug     1380

gaggcuauuc uccaccaaca ggccaaauau uacccguucc ugaaggaaaa cuaugauaaa     1440

aucaaguccc uugucaccuu ucgcauuccc uacuucgugg gaccucuggc uaauggccag     1500

agcgaguuug ccuggcucac ccggaaagcg gacggggaga ucagacccug gaacauugaa     1560

gagaagguug acuucggaaa aucugccgug gauuuuaucg aaaagaugac aaauaaggac     1620

acguaucugc cuaaagagaa cguccugccc aagcacuccc ucugcuacca aaaauaccug     1680

guguauaaug aacuuaccaa gguucgcuac aucaacgauc agggcaaaac aagcuauuuc     1740

ucuggccagg agaaggagca gauuuucaau gaccuguuua agcaaaaacg gaaggugaaa     1800

aagaaagauc ucgaacuguu ccugagaaac augagccaug ucgagucccc gaccaucgaa     1860

gggcuugagg acucuuuuaa uagcuccuac agcacauauc acgaucuccu gaagguggga     1920

auuaagcagg aaauccugga caacccuguc aauaccgaga ugcucgagaa cauugugaaa     1980

auccugacgg uuuucgaaga uaagcgcaug aucaaagagc agcuucagca auuuucugac     2040

gugcuggaug gcgucgugcu caagaaacug gaacggagac auuacacagg guggggacgc     2100

cuguccgcua agcuccucau gggcauucgg gacaagcaga gccaccugac cauccuggau     2160

uaucucauga augacgacgg gcugaacaga aaccuuaugc agcugauuaa ugauucuaac     2220

cucuccuuca aaagcaucau ugagaaggag cagguuacca cagccgacaa agauauccaa     2280

uccaucgugg cugaccuggc cggcucuccc gcgauuaaga aaggaauccu gcagagccuu     2340

aagauugucg augaacucgu guccgugaug ggcuacccuc cccagaccau cguugucgag     2400

auggcccggg aaaaucagac aacggggaaa ggaaagaaca auucucgccc cagauacaag     2460

agccuggaga aagcuauuaa ggaauuuggc ucccaaaucc ugaaagagca uccuaccgac     2520

aaccaggagc uccggaauaa cagacuguau cuuuacuauc ugcagaaugg gaaggauaug     2580

uacacaggac aagaccucga uauccacaac cugagcaauu augacauuga ucacaucgug     2640

ccgcagucuu ucauuaccga caacuccauc gauaaucugg ugcuuacaag cucugccggc     2700

aaccgcgaaa aaggggacga cgugcccccu cucgagauug ugcggaagag aaagguguuu     2760

ugggaaaaac uguaccaggg caaucugaug uccaagcgca aauucgauua ucucaccaag     2820

gcugagagag gaggccugac ggaagccgac aaagcccggu uuauccaucg ccagcuuguu     2880

gagaccagac aaauuacaaa gaacgucgcu aacauccugc accagcgguu caauuacgag     2940

aaggaugacc augggaacac caugaaacag gugcgcaucg uuacacucaa gagcgcgcug     3000

gugucucagu uuagaaaaca auuccagcug uacaaggucc gggaugugaa ugacuaucac     3060

cacgcccaug augcuuaccu uaacggaguu guggccaaua cccugcucaa agucuauccc     3120

cagcuggaac cugaguucgu guacggcgac uaucaccagu uugauugguu caaggccaac     3180

aaggcuacgg ccaaaaagca auuuuacaca aauauuaugc uuuucuuugc gcagaaagac     3240

cgcaucauug augaaaacgg ggagauccug ugggacaaga aauaucuuga caccgugaag     3300

aaggucauga gcuacagaca gaugaauauu guuaaaaaga cagaaaucca aaaaggagag     3360

uucuccaagg cuaccaucaa acccaagggc aacagcucua aacugauucc gcggaagacc     3420

aauugggauc cuaugaagua cgggggccuc gacuccccca acauggccua ugccguggug     3480

aucgaguacg cuaaaggaaa gaauaaacug gucuuugaaa agaaaauuau ccgcgugaca     3540

auuauggaga gaaaggccuu cgaaaaggau gagaaagcgu uucuggagga acagggcuau     3600

cggcagccua agguucuugc uaaacugccc aaguacacgc ucuaugagug ugaagagggg     3660

agacgccgga ugcuggccag cgccaacgaa gcucagaaag gaaaucaaca ggugcucccu     3720

aaccaucugg ucacccuucu gcaccaugcc gcuaacugcg agguguccga cggcaagucu     3780

cucgauuaca ucgagagcaa uagagaaaug uucgccgagc ugcuggcgca cguuuccgaa     3840

uuugccaagc gcuauacacu ugcugaggcc aaccugaaua aaaucaacca gcucuuugaa     3900

cagaauaagg agggggacau uaaagcuauc gcccaaucuu ucguggaucu gauggccuuu     3960

aacgcuaugg gagcgcccgc cagcuucaag uuuuucgaga ccacaauuga acggaaaaga     4020

uacaauaacc ucaaggagcu gcuuaauucc accaucauuu accagucuau cacgggccug     4080

uaugaaagcc gcaagcggcu ggacgauaaa agaccggcug ccacaaagaa agccgggcag     4140

gcuaagaaaa agaaauaagg acuagugcau cacauuuaaa agcaucucag ccuaccauga     4200

gaauaagaga aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu     4260

guaaagccaa cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc     4320

ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  4428


<210>  4738
<211>  4461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4738
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccaa gaaaccguac      120

agcauuggac uggacaucgg caccaacucc guuggguggg cgguggugac agaugacuau      180

aagguccccg cuaagaaaau gaaggugcuc ggaaauacgg auaaaucuca uauugagaag      240

aaccugcuug gcgcccuccu guucgacagc gggaauaccg ccgaagaucg gcgccugaaa      300

agaacagcuc ggcgcagaua cacccggcgc agaaaccgga uccuuuaucu gcaggagauu      360

uucuccgaag agaugggaaa gguugacgau ucuuucuuuc accgccucga agacagcuuc      420

cuggugacag aggauaagag aggcgagcgg cauccuaucu uuggcaaucu ggaagaggaa      480

gucaaauacc acgagaacuu ccccaccauc uaucaucucc gccaauaccu ggccgacaau      540

ccugagaagg uggaucuuag acugguuuau cucgcucugg cccacauuau caaauuucgg      600

gggcacuucc uuauugaagg aaaguuugac acacgcaaca augaugugca gagacuguuc      660

caggaguuuc ucgcggucua cgacaacacc uucgaaaauu ccucucugca ggagcaaaac      720

guccaggugg aagagauccu gacggacaaa auuagcaagu ccgccaaaaa ggaucgggug      780

cucaagcugu uucccaauga gaaaagcaac ggcagauucg cugaauuucu uaagcugauc      840

guugggaauc aggccgacuu uaaaaagcau uucgagcucg aagagaaagc uccucugcag      900

uuuucuaagg auaccuacga agaggagcuu gaagugcugc ucgcccaaau uggagacaac      960

uaugcggagc uguuccuguc cgccaagaaa cucuacgaua gcauccugcu uucuggcauc     1020

cugacaguga ccgacgucgg gacaaaggcu ccgcucuccg ccagcaugau ucagcgcuau     1080

aacgaacacc agauggaucu ggcucagcuu aaacaauuua uccggcagaa gcugucugac     1140

aaauacaaug agguguucuc cgauguuagc aaggacggau augccggcua cauugauggc     1200

aagaccaacc aggaagccuu uuauaaauac cucaaggggc ugcugaauaa aaucgaggga     1260

uccggcuacu uccucgacaa gauugagaga gaagacuuuc ugcgcaaaca acggacguuc     1320

gauaacgggu cuauccccca ucagauccac cuucaggaga ugagagcuau uauccgccgg     1380

caggcggaau uuuauccuuu ccuggccgac aaucaagaua gaauugagaa gcuccugaca     1440

uuucgcaucc ccuacuaugu gggaccucug gcucggggca agagcgacuu cgccuggcuu     1500

uccagaaaaa gcgccgauaa gauuaccccc uggaacuucg acgagaucgu cgauaaagaa     1560

ucuuccgcug aggccuuuau caaucgcaug acaaacuacg accucuaucu gccgaaucag     1620

aaggugcugc cuaaacacag ccuccuguac gaaaaguuca ccguuuauaa cgagcuuacc     1680

aaagugaagu acaagacaga acaggggaaa acggcguuuu ucgaugcuaa uaugaagcag     1740

gagauuuuug acggagucuu caaaguguac cggaagguca ccaaagauaa gcugauggac     1800

uuucucgaga aggaguucga ugaauuuaga aucguggacc ugacaggccu ggacaaagaa     1860

aacaagguuu ucaaugccuc uuauggcacc uaccaugauc uuugcaaaau ucucgacaag     1920

gauuuucugg acaacagcaa aaaugagaag auccuggaag auauugugcu cacacugacc     1980

cuuuucgagg accgggagau gauccgcaag agacuggaaa acuauuccga ucuccugacg     2040

aaagagcaag ucaagaaacu ggaacggaga cacuacacag gguggggacg ccuuucugcc     2100

gagcucauuc auggcauccg gaacaaggaa agcagaaaaa ccauccugga cuaucugauu     2160

gaugacggaa auuccaaccg caauuuuaug cagcucauca acgaugacgc ucugagcuuc     2220

aaggaggaga uugccaaggc ucaggugauc ggggaaaccg auaaucuuaa ccagguugug     2280

ucugacauug ccggcucccc cgcgaucaaa aaggggaucc ugcaaagccu caaaauuguc     2340

gacgagcugg ugaagaucau gggccaccag ccugaaaaua uugugguuga gauggccaga     2400

gagaaccagu ucacaaauca gggacggcgc aacucucaac agagacugaa aggccuuacc     2460

gauuccauca aggaauuugg gagccagauu cucaaagagc aucccgucga aaauucccaa     2520

cugcagaacg accggcuguu ccucuacuau cugcagaaug gacgcgauau guacacaggc     2580

gaggaacuug acaucgauua ccugucucag uaugacaucg aucacauuau cccccaggcu     2640

uuuauuaagg acaacagcau cgauaauaga gugcucacgu ccucuaagga gaaccggggg     2700

aaaagcgacg augugccuuc caaggacguc gugcgcaaaa ugaaaagcua cuggucuaaa     2760

cugcuguccg ccaaacuuau uacccaaaga aaguucgaca accugacaaa agcugagcgg     2820

ggaggccuca ccgaugacga uaaggccggg uuuaucaaac gccagcuggu ugaaacaaga     2880

cagaucacca agcacguggc gcggauucuc gacgagagau ucaauacgga aaccgaugag     2940

aacaauaaaa agauccgcca agucaagauu gugacacuga aaagcaaccu uguuucuaau     3000

uuucggaagg aguucgaacu guauaaagug agagagauca acgacuacca ucacgcccau     3060

gaugcuuauc ucaaugccgu cauuggcaag gcucugcugg gaguguaccc gcagcuugaa     3120

cccgaguuug uuuauggcga cuacccucac uuccacgggc auaaagaaaa caaggccacc     3180

gccaagaaau uuuucuacuc caauaucaug aacuucuuua agaaagauga cgugcgcaca     3240

gauaagaaug gagagauuau cuggaaaaag gacgaacaca ucagcaacau uaagaaaguc     3300

cugucuuauc cccaggugaa uaucgugaag aaagucgagg agcagaccgg cggguucagc     3360

aaggaaucca uucucccuaa aggaaacagc gauaagcuga ucccccggaa aacgaagaag     3420

uuuuacuggg acacaaagaa guauggcggg uucgacucuc cgauuguugc uuacuccauc     3480

cucgugaucg cggauauuga gaaaggcaag agcaaaaagc ugaagaccgu gaaagcccuu     3540

gucggaguga caaucaugga aaagaugacc uuugagagag acccuguugc uuuccuggag     3600

cgcaaaggcu aucggaaugu gcaagaagag aacauuauca agcuccccaa auacucccug     3660

uuuaagcugg aaaacgggag aaagcgccuu cuggccucug cccgggagcu ccagaaagga     3720

aaugaaauug uccugccuaa ccaucucggc acccugcuuu aucacgcuaa gaauauccau     3780

aaaguggaug agcccaagca ccuggacuac guugauaaac acaaggacga guucaaggaa     3840

cugcucgaug uggucagcaa cuuuuccaaa aaguacacac uggccgaggg gaaucuugaa     3900

aagaucaagg agcuguaugc gcagaacaau ggagaagacc ucaaagagcu ggcuucuagc     3960

uucauuaacc uccugacguu uaccgccauc ggcgccccug cuacauucaa guuuuucgau     4020

aaaaauauug acagaaagcg guacaccucc acaaccgaga uccuuaacgc cacgcugauu     4080

caucaaucua ucacagggcu guaugaaacc cgcauugauc ucaauaagcu gggcggagac     4140

aaaagacccg cggcuaccaa gaaagccggc caggccaaga aaaagaagua aggacuagug     4200

caucacauuu aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa     4260

uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac     4320

auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa     4380

gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaagaau u                                               4461


<210>  4739
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4739
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccca gacaacaaac      120

cugagcuaca uucucggacu ggaccuuggc aucgcguccg uugggugggc ugugguggag      180

auuaaugaaa acgaggaucc gaucggacug auugacgucg gcgugcggau cuucgaacgc      240

gccgagguuc ccaagacggg ggaaucucuc gcccugagca gacggcuugc ucgcuccacc      300

agacggcuga uccgcagacg ggcccaucgc cuccugcugg cuaaaagauu ucucaagcgg      360

gaggggauuc ugucuacaau cgaucuugag aagggccugc cuaaucaagc cugggaacuc      420

cgcguggcgg gccuggagag acggcuuagc gccauugaau ggggagcugu ccugcuccac      480

cugaucaaac aucgcggaua ucuguccaag cggaaaaacg agucucagac caauaacaag      540

gagcucggcg cccugcuuag cgggguggcu cagaaucacc agcugcucca auccgacgau      600

uacagaacac ccgccgaacu ggcgcuuaaa aaguucgcca aggaggaagg acauauuaga      660

aaccagcgcg gcgcuuauac ccacacauuu aaucggcugg accuccuggc cgagcugaac      720

cuccuguucg cucagcagca ccaauuuggg aauccucauu gcaaagaaca cauccagcag      780

uacaugaccg agcuucugau guggcagaag cccgcccuca gcggagaggc cauucugaaa      840

augcuuggca aguguacgca ugaaaagaac gaguucaaag cugcgaaaca caccuauucu      900

gccgaaagau uuguuuggcu gacaaagcuc aauaaccugc gcauccugga ggauggcgcu      960

gaacgggccc ucaaugagga ggaaagacaa cugcuuauca accacccuua cgagaagucc     1020

aaacugaccu augcccaggu gcgcaagcuc cuggggcuga gcgaacaggc uauuuucaaa     1080

caucuucggu acucuaagga gaacgccgaa uccgcgacau uuauggagcu caaggcuugg     1140

cacgccauca gaaaagcccu ggagaaucaa ggacugaagg acaccuggca ggaucucgcu     1200

aaaaagccgg accugcuuga ugaaauuggc acggccuuca gccuguacaa aacagacgag     1260

gauauccagc aguaucucac caacaagguc cccaauucug ugauuaacgc ucugcuggug     1320

ucccuuaauu uugacaaauu caucgaacuc agccugaagu cccugcgcaa gauccucccu     1380

cugauggagc aagggaaacg guacgaccag gccugcagag agauuuaugg acaucacuac     1440

ggcgaagcga accagaagac aucucagcuu cugcccgcca ucccugcuca agagauucgc     1500

aaucccgucg uucuccggac ccugagccag gccagaaaag ugaucaacgc uauuauccgg     1560

caguaugggu ccccggcccg cgugcauauc gaaaccggaa gagagcuggg caagagcuuu     1620

aaagaacgga gagagauuca gaagcaacag gaggauaauc gcacaaagcg ggaaucugcg     1680

guccagaaau ucaaggagcu uuucuccgac uuuagcucug aaccuaaaag caaggauauc     1740

cucaaauuca gacuguacga gcagcaacac ggcaaguguc uguauuccgg gaaagaaauu     1800

aacauccacc gccucaauga gaagggauac guggagauug accaugcccu gcccuuuucu     1860

agaacguggg augacagcuu caacaauaag guucuugugc uggcuuccga aaaccagaau     1920

aaaggcaacc agaccccuua cgaguggcuc caagggaaga ucaacagcga acgguggaaa     1980

aauuuugucg cccuggugcu gggaucucag ugcuccgcug ccaagaaaca gcgccuucuc     2040

acacagguua uugaugacaa caaguucauc gauagaaauc ugaacgacac ccgguauauc     2100

gcccgcuuuc ugagcaauua cauucaagag aaccuccugc uugugggcaa gaauaaaaag     2160

aacgucuuca cacccaaugg gcagaucacc gcucugcuca gaucucggug gggccugauu     2220

aaagcgcgcg aaaacaauaa cagacaccau gcccuggaug cuaucguggu cgccugugcc     2280

acgcccucca ugcagcagaa gauuacacgg uuuauccgcu ucaaagaggu gcacccuuau     2340

aagaucgaga auagauacga aaugguugac caagagagcg gagaaauuau cuccccgcac     2400

uuucccgagc cuugggcuua uuuccggcag gaggugaaca uuagagucuu ugacaaucau     2460

cccgauaccg ugcuuaagga aaugcugccu gaccgccccc aggccaacca ccaguucguu     2520

caaccucucu ucgugucucg ggcgccgacc agaaaaauga gcggccaggg gcauauggag     2580

acaaucaagu ccgcuaaacg ccuggccgaa ggaauuucug uccuccggau cccccugacc     2640

cagcuuaagc cuaaccugcu cgagaauaug gugaacaaag aaagagagcc cgcccuguac     2700

gcuggccuga aggcccgccu ugcggaguuu aaucaagauc cugcuaaggc cuucgccaca     2760

cccuuuuaua aacaaggggg acaacaggug aaggcuaucc ggguugaaca gguccagaaa     2820

agcggcgugc uggugagaga gaacaauggg gucgccgaca acgcuuccau ugugcgcacg     2880

gauguuuuca ucaagaauaa caaauuuuuc cucgugccga uuuacaccug gcaggucgcc     2940

aagggcaucc ugccuaauaa agcgauugug gcccacaaga acgaagacga gugggaagag     3000

auggaugagg gagcuaaguu uaaauucagc cucuuuccca augaccuggu ugaacuuaag     3060

accaaaaagg aguacuucuu uggcuauuac aucgggcugg aucgggccac aggaaacauc     3120

ucucucaaag aacaugacgg cgagauuucc aaggggaagg auggagugua uagagucggc     3180

gugaaacugg cucugagcuu cgaaaaguac cagguugacg agcuugggaa aaaucggcag     3240

aucugccgcc cccagcagag acagccugug cggaagcgcc cggccgccac caaaaaggcu     3300

ggccaagcga agaaaaagaa auaaggacua gugcaucaca uuuaaaagca ucucagccua     3360

ccaugagaau aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc     3420

guugguguaa agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu     3480

uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           3594


<210>  4740
<211>  3672
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4740
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccgc ggcuuucaag      120

ccgaacccca uuaauuacau ccugggacuc gacauuggca ucgccagcgu ugggugggcc      180

augguggaga uugaugaaga cgagaacccu aucugccuga ucgaucuugg agugcggguc      240

uuugaacgcg cugaggugcc caaaaccggc gacucccugg ccauggcuag acggcucgcc      300

cgcucuguua gacggcugac acgcagacgg gcgcaucgcc uucugagagc ccggcgccuc      360

cugaagagag aaggggugcu gcaggcugcc gauuucgacg agaauggacu cauuaagagc      420

cugccuaaca cgcccuggca acuucgggcu gccgcgcugg aucgcaaacu caccccucug      480

gagugguccg ccguccuucu gcaccucauc aagcauagag gcuaucuguc ucagcggaaa      540

aaugaaggcg agacagcuga caaggaacug ggggcccucc ugaaaggagu ggcugauaac      600

gcccacgccc uucagaccgg cgacuuuaga acaccggcug agcuggcgcu caauaaguuc      660

gagaaggaaa gcgggcauau ucgcaaccag cggggagauu acucccacac cuuuucuaga      720

aaagaccugc aagccgagcu uauccuccug uucgaaaagc agaaagaguu uggcaauccc      780

cacguuagcg ggggacugaa ggaaggcauu gagacacugc ucaugaccca gcgcccugcu      840

cuguccggcg augccgugca gaaaaugcuu gggcauugua cguucgagcc cgccgaaccu      900

aaggcugcca aaaacaccua uacagcggag cgguuuaucu ggcugaccaa gcucaauaac      960

cugcgcauuc uugaacaagg aagcgagaga ccccugacag acaccgaacg ggcuacgcuc     1020

auggacgagc cguacagaaa gucuaaacug acauaugccc aggcccgcaa gcugcucggc     1080

cuggaggaua ccgcuuucuu uaaagggcuu cgguacggaa aggacaaugc cgaggcuucc     1140

acacugaugg aaaugaaagc cuaucacgcg aucagcagag cccucgaaaa ggagggccug     1200

aaggauaaaa agucuccucu gaaccuuucc cccgaacucc aggacgagau cgggaccgcu     1260

uucagccugu uuaaaaccga ugaggacauu acaggacgcc ugaaggaucg gauccagccu     1320

gaaauucucg aggcccugcu uaaacauauc ucuuucgaca aguucgucca aauuucccug     1380

aaggcucuca gacggaucgu gccccugaug gaacagggca aacgcuacga ugaggccugc     1440

gcggagaucu acggcgacca cuaugggaag aaaaauacgg aagagaagau uuaccugccc     1500

ccuaucccgg ccgaugaaau uagaaacccc gugguccuuc gggcucucag ccaggccaga     1560

aaaguuauca acggaguggu gcgccgguau ggcuccccug cucgcauuca caucgagacc     1620

gccagagaag ucgggaaguc uuuuaaggac agaaaagaga ucgagaagcg gcaagaagag     1680

aaucgcaaag acagagaaaa ggccgcugcg aaauuccggg aguacuuucc caacuucgug     1740

ggagaaccua agagcaaaga uauucugaag cugcgccucu augagcagca gcauggcaag     1800

ugucuguacu ccgggaaaga gaucaaucuu ggcagacuga acgaaaaggg auauguugag     1860

auugaccacg cccuccccuu uagccggaca ugggaugacu cuuucaauaa caaagugcug     1920

guccugggcu ccgaaaauca gaacaagggg aaucaaaccc cguacgagua cuuuaacgga     1980

aaagauaaua gccgcgaaug gcaggaguuc aaggcuagag uggagacauc ucgguuuccu     2040

cgcagcaaga aacagagaau ccuucuccag aaguucgacg aagauggcuu uaaagagcgg     2100

aaccugaaug acaccagaua uguuaaccgc uuccugugcc aauuuguggc cgaucggaug     2160

agacucacgg ggaagggcaa aaagcgcguc uucgccucca auggacagau uacaaaccug     2220

cuucgggggu ucuggggccu gagaaaggug cgcgcugaaa acgaccggca ucacgcccuc     2280

gaugcggucg ugguugcuug uucuaccgug gccaugcagc agaaaaucac cagauuuguc     2340

cgcuacaagg agaugaaugc cuucgacgga aaaacaauug auaaggagac cggcgaagug     2400

cugcaucaga aaacacacuu uccccagccu ugggaguucu uugcucagga aguuaugauc     2460

cggguguucg ggaagcccga cggaaagccu gaguuugaag aggccgacac gcccgagaaa     2520

cugagaaccc uucucgcgga aaagcugagc ucccggccgg aggcugucca cgaauaugug     2580

acaccucugu ucgugagccg cgcccccaac agaaaaaugu cuggccaggg gcauauggag     2640

accguuaagu ccgccaaacg gcucgaugaa ggagucagcg ugcugcgcgu gccccuuaca     2700

caacugaagc ucaaagaccu ggagaagaug gucaauagag agcgggaacc uaagcuguac     2760

gaggcucuua aagccagacu ggaagcucac aaggaugacc cggccaaagc guuugccgag     2820

cccuucuaua aguacgauaa agcuggcaac cgcacccaac aggugaaggc cguucgggug     2880

gaacaagucc agaagacggg gguguggguu agaaaucaua acggcaucgc ugacaaugcc     2940

accauggugc gcgucgaugu guuugagaaa ggagacaagu auuaccucgu uccuauuuac     3000

ucuuggcaag uggccaaagg cauccugccc gaucgggcug ucgugcaggg gaaggacgag     3060

gaagauuggc agcucauuga cgauuccuuc aacuuuaaau ucagccugca cccuaaugac     3120

cuuguggagg ucaucacaaa gaaggcgaga auguucggau auuuugccuc cugccaccgc     3180

ggcaccggga acauuaauau ccggauccau gaccuggauc acaaaauugg aaagaacggc     3240

auccucgaag ggauuggcgu uaaaacagcu cugucuuucc agaaguacca gaucgacgag     3300

cugggaaaag agauuagacc cugucgccuu aagaagagac cucccgugcg gaaacgcccg     3360

gccgccacca agaaagcugg ccaggccaag aaaaagaagu aaggacuagu gcaucacauu     3420

uaaaagcauc ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu     3480

caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc     3540

uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga     3600

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaagaa uu                                                         3672


<210>  4741
<211>  3678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4741
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccaa guaccauguu      120

ggaauugacg ugggcaccuu cagcgugggg cuggcggcua ucgaggucga ugacgccgga      180

augccgauua aaacacucuc ccuggugucu cacauccaug auagcggccu ugaccccgau      240

gaaauuaagu ccgccguuac gcggcuggcu ucuagcggga ucgcccgcag aacccggcgc      300

cucuauagac ggaagcgcag acggcugcag caacuugaca aauuuaucca gcgccaggga      360

uggccuguga uugagcugga agauuacucc gacccccucu auccuuggaa ggucagagcu      420

gagcuggccg cgucuuacau cgccgaugaa aaagagcggg gcgagaagcu gagcguggcu      480

cuccgccaca uugccagaca ucggggcugg cgcaaccccu augcuaaagu uuccagccug      540

uaccuuccug acgggccguc ugaugccuuc aaggcgauca gagaagagau uaagcgggcc      600

uccggacagc ccgugccuga aacagcuacc gucggccaaa uggugacacu gugcgagcuc      660

gggacccuga aacuuagagg agagggcggg gugcugagcg cccgccucca gcagucugac      720

uaugcucggg aaauccagga gauuuguaga augcaagaaa ucggacagga gcuguaccgc      780

aagaucauug augucguuuu ugccgccgaa ucccccaaag gcagcgcuuc uucccgggug      840

ggcaaggacc cucugcagcc cgggaaaaau agagcgcuca aggccagcga cgcuuuccag      900

cgcuaccgga ucgccgcccu gauuggaaac cuuagagugc gcgucgaugg cgagaaacgg      960

auccuguccg uggaggaaaa gaaucucguu uuugaccacc uggugaaccu uacaccgaag     1020

aaagagccug aaugggucac cauugcugag auccugggga ucgauagagg acaacucauu     1080

ggcacggcca ccaugacaga cgauggggaa cgcgcgggag cucggccccc uacccacgac     1140

acaaauagau cuaucgugaa cagccggauu gcuccccugg uugauuggug gaagaccgcc     1200

uccgcccugg agcagcaugc cauggugaaa gcucucagca augccgaggu cgacgauuuc     1260

gacucucccg aaggcgcgaa ggugcaggcc uuuuucgcug aucuggacga cgauguccac     1320

gccaaacuug acucccugca ucucccugug ggccgcgcug ccuauagcga ggauacgcug     1380

guuagacuua cacggagaau gcugucugac gggguggauc ucuacaccgc gcgccugcaa     1440

gaauuuggaa ucgagccgag cuggacaccc ccuacacccc ggauuggcga accugucggg     1500

aaccccgccg uggacagagu ucugaagacc gugucccgcu ggcucgaguc ugcuaccaag     1560

acguggggag ccccggagag agucaucauc gaacacgugc gggagggcuu cgugaccgaa     1620

aaacgcgcua gagagaugga uggggacaug cggcgcagag ccgcccggaa ugcuaagcug     1680

uuucaggaga ugcaggaaaa acuuaacguu cagggcaagc cuagccgcgc ggaucugugg     1740

agauaucaau ccguccagcg gcagaauugc cagugugccu acugcggaag ccccauuaca     1800

uucucuaacu ccgagaugga ccacaucgug ccucgcgcug gccaagggag caccaauaca     1860

agagaaaacc ucguggccgu cugucaucgg ugcaaucagu cuaaaggaaa cacccccuuu     1920

gccauuuggg cuaagaacac guccaucgag ggcgugagcg uuaaggaagc cguggagaga     1980

acacgccacu gggucaccga uaccgggaug cgguccacag acuucaaaaa guucaccaaa     2040

gcggugguug agagauuuca gcgcgcuaca auggaugaag agauugacgc ccggucuaug     2100

gaaagcgugg ccuggauggc uaaugagcug agaucccgcg ucgcccagca uuucgcgucu     2160

cacggaacga ccgugcgggu uuauagaggc agccugacag cugaagcccg ccgggccucc     2220

gggaucagcg gcaagcugaa auuuuucgac ggagugggca agucuagacu cgaucggcgc     2280

caucacgcua uugacgccgc ugucaucgcc uuuaccuccg auuacguggc ggagacacug     2340

gccgugagaa gcaaccugaa gcaaucucag gcucaccggc aggaggcccc ucaguggcgc     2400

gaauuuaccg ggaaagacgc ugagcauaga gccgccuggc gggucuggug ucaaaagaug     2460

gaaaaacucu ccgcucugcu uacggaggau cugagagacg aucgcguugu ggugaugagc     2520

aauguccggc ucagacuggg aaacggcucu gcgcacaagg aaaccaucgg gaaacugagc     2580

aaggugaaac uuuccagcca gcucucuguu uccgacauug auaaggccag cuccgaggcu     2640

cuguggugcg cccugacacg cgagcccgga uuugacccga aggaaggccu cccugccaau     2700

cccgagcggc auaucagagu gaacgggacc cacgucuaug cuggcgauaa uauuggacug     2760

uuccccgugu cugccggcag caucgcgcuu cgcgggggau acgcugaacu gggcuccucu     2820

uuucaccaug cccggguuua caaaauuaca agcgggaaga aaccugccuu cgcuaugcuc     2880

agaguguaua ccaucgaccu gcugccguac cgcaaccagg aucuuuucuc cgucgagcuc     2940

aagccccaaa cgaugucuau gagacaggcc gagaaaaagc ugcgggacgc gcuggcuaca     3000

ggaaaugccg aauaucucgg cuggcuggug guugacgaug agcuuguggu cgacaccagc     3060

aagaucgcca cagaucaggu gaaagcugug gaagccgagc uggggaccau ucgcagaugg     3120

cgggucgacg gcuucuuuuc cccuagcaag cuccgccuga gaccccugca gaugucuaaa     3180

gaaggaauca agaaagaguc cgcuccugag cuuagcaaga uuaucgaucg gcccggcugg     3240

cucccugccg uuaacaagcu guucucugac gggaauguga ccguggucag acgcgauucc     3300

cugggacggg ugagacucga aagcacagcg caccuucccg uuacguggaa agugcaaaag     3360

cgcccggccg cuaccaaaaa ggccggccag gcuaaaaaga agaaauaagg acuagugcau     3420

cacauuuaaa agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag     3480

cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua     3540

aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa     3600

ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaagaauu                                                   3678


<210>  4742
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4742
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccgc gcggauucug      120

gcuuucgaca ucggaauuag cuccaucggc ugggccuuuu cugagaacga ugaacucaag      180

gacugcgggg uucgcauuuu caccaaagug gagaauccga agacaggaga aagccuggcc      240

cuucccagac ggcuggcucg cuccgccaga aagcggcucg cucgcagaaa agcccggcug      300

aaccaucuua agcaccugau cgcgaaugag uuuaaacuca acuacgaaga uuaucagucu      360

uucgacgaga gccuggccaa ggcuuacaaa ggcucccuga ucucuccuua ugagcuccgc      420

uuuagagccc ugaaugaacu ucugagcaag caagauuucg cucgggugau ucuccauauc      480

gccaagcgca gaggguacga cgauauuaaa aacuccgacg auaaggagaa aggagcgauc      540

cugaaggcca uuaaacagaa ugaagagaaa cuugcuaacu aucagagcgu cggcgaguac      600

cuguauaaag aauacuuuca gaaguucaag gaaaauucua aagaauuuac gaacgugcgg      660

aauaagaaag aguccuacga acgcuguauc gcccagagcu uccucaagga cgagcugaaa      720

cugauuuuua agaagcaaag agaguucggc uuuucuuucu ccaaaaaguu cgaagaggaa      780

guucucagcg uggcuuuuua uaaacgggcc cugaaggauu ucucucaccu ugucgggaac      840

ugcuccuuuu ucaccgacga gaaaagagcc cccaagaaua gcccucuggc uuuuauguuc      900

guggcgcuca cacgcaucau caaccugcuu aauaaccuga agaacaccga aggcauucuc      960

uacacaaaag augaccugaa ugcccugcuc aacgagguuc ugaagaaugg aacccuuaca     1020

uauaaacaga ccaagaaacu gcucgggcug uccgacgauu acgaguuuaa gggagaaaag     1080

ggcacguauu ucaucgaguu uaaaaaguac aaagaguuca uuaaggcucu uggggagcau     1140

aaccugucuc aggacgaucu caaugaaauc gccaaagaca uuacccugau caaggaugag     1200

aucaaacuga agaaggcccu cgcuaaauau gaccugaacc aaaaucagau ugauagccuu     1260

uccaagcugg aguuuaaaga ccaccucaac aucagcuuca aggcccugaa acuggugaca     1320

ccccuuaugc ucgaaggaaa gaaguacgau gaggcgugua augaacugaa ccugaaaguc     1380

gcuauuaaug aggacaagaa agauuuucuc ccugccuuca acgagaccua cuauaaggac     1440

gaagugacaa auccgguggu ccugcgggcc aucaaagagu acagaaaggu ucuuaacgcu     1500

cugcucaaga aauauggcaa ggugcacaaa auuaauaucg aacuggcccg cgaggugggc     1560

aagaaccauu cucagcgggc uaaaaucgaa aaggagcaga acgagaauua caaggccaaa     1620

aaggacgcgg aacuggagug cgaaaaacuu gggcucaaga uuaacuccaa aaauauccug     1680

aagcugagac ucuucaaaga gcaaaaggaa uuuugugccu auagcggaga gaagauuaaa     1740

aucucugauc ugcaggacga gaagaugcuu gaaauugauc acaucuaccc cuauagccgc     1800

uccuucgacg auucuuacau gaacaaaguc cugguguuua ccaagcagaa ucaagagaaa     1860

cucaaccaga cgccuuucga ggcuuuuggc aaugacagcg ccaaguggca gaagauugaa     1920

guucuggcua aaaaccugcc cacaaagaaa cagaagcgga uccuugauaa aaauuacaag     1980

gacaaggaac aaaagaacuu caaggauaga aaucucaacg acacccgcua uaucgcccgg     2040

cuggugcuga auuacacaaa agauuaucuc gacuuucugc cucuuuccga ugacgagaac     2100

accaagcuga augacaccca gaaagggagc aagguccaug uggaggcgaa gucuggaaug     2160

cucacauccg cccugagaca cacguggggc uucagcgcua aagaucgcaa caaccaucug     2220

caccacgcca uugacgcugu uaucauugcc uacgccaaua acucuaucgu gaaggcuuuu     2280

uccgauuuca aaaaggaaca ggagagcaau uccgcggaac uuuaugccaa aaagauuucu     2340

gagcucgacu acaagaacaa acggaaguuu uucgagcccu uuagcggguu cagacagaaa     2400

guccuggaua agaucgacga aaucuucgug uccaaaccgg agcggaagaa accuucugga     2460

gcucugcaug aagagaccuu ucgcaaggaa gaggaguucu aucaaagcua cggcggcaag     2520

gaaggggucc ucaaagcccu ggagcuugga aagauuagaa aagugaaugg caagaucguu     2580

aaaaacgggg auauguuucg gguggacauu uucaagcaca agaaaacaaa uaaguuuuac     2640

gccgucccca ucuauaccau ggauuucgcu cugaaagugc ucccuaacaa ggccguugcg     2700

agauccaaaa agggagaaau uaaggacugg auccugaugg augagaauua cgaauuuugc     2760

uucagccugu auaaagacuc ucuuauccug auucagacaa aggauaugca ggagcccgag     2820

uuuguguacu auaacgcuuu caccuccagc acggucucuc ucaucguguc caaacaugac     2880

aauaaguuug aaacacugag caaaaaccag aagauucucu ucaagaaugc caacgagaaa     2940

gaagugaucg ccaagucuau uggcauccaa aaucugaaag uuuucgagaa guacaucguc     3000

agcgcucuug gggaagugac caaagccgag uuucgccagc gggaggacuu caagaaaaag     3060

agacccgcgg cuaccaagaa agccggccag gccaagaaaa agaaauaagg acuagugcau     3120

cacauuuaaa agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag     3180

cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua     3240

aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa     3300

ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3360

aaaaaaaaaa aaagaauu                                                   3378


<210>  4743
<211>  3789
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4743
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccag cgaccugguu      120

cucggacugg auauuggcau cggguccgug ggagugggca uucuuaacaa ggucaccggg      180

gagaucauuc auaaaaauuc ucggaucuuc ccggcggcuc aggccgaaaa caaucuggug      240

cgcagaacaa accggcaagg acgcagacuc gcccggcgca agaagcacag acggguucgc      300

cugaauagac uuuuugagga aagcggccug aucacggacu ucaccaaaau uuccaucaac      360

cucaaucccu accagcugcg ggugaagggc cugacagaug agcucucuaa cgaggagcug      420

uuuauugcuc uuaaaaauau ggucaagcau cgcgggauca gcuaucugga cgaugccucc      480

gacgauggaa acucuagcgu gggcgacuac gcucagauug uuaaagaaaa uuccaagcag      540

cucgaaacca agacaccugg gcaaauccag cuggagagau aucagaccua cggacagcuu      600

cggggcgauu ucacagugga aaaagacggg aagaaacacc gccugauuaa cgucuuuccc      660

accagcgccu auagaucuga ggcgcuccgg auccugcaaa cgcagcagga guucaauccu      720

cagaucaccg augaauuuau uaacagauac cuggagaucc ucacaggaaa gcgcaaauau      780

uaccauggcc ccggcaauga aaagucccgg accgacuacg ggagauaucg cacaagcgga      840

gagacccugg auaacauuuu cggcauccuu auugggaaau gcacguuuua cccugacgag      900

uuccgggccg cuaaggccuc uuauacagcu caagaauuua accugcucaa ugaccugaac      960

aaucuuaccg ugccgacaga gaccaagaaa cuguccaagg aacagaaaaa ccagaucauc     1020

aauuacguga agaacgagaa agccauggga cccgcgaaac ucuucaagua uauugccaaa     1080

cugcugagcu gugaugucgc ugacaucaag ggcuacagaa uugauaaauc ugggaaggcc     1140

gaaauccaca ccuuugaggc uuaucgcaaa augaagacac ucgaaacgcu ggacauugag     1200

caaauggauc gggagacccu ugacaagcug gccuacguuc ucacacugaa uaccgaaaga     1260

gagggaaucc aggaagcccu ugagcacgag uucgcugaug gcuccuucag ccagaaacag     1320

guggacgaac uggugcaauu ucgcaaggcg aacuccucua ucuucggcaa aggguggcau     1380

aauuuuagcg ucaagcucau gauggagcug auuccugaac uguacgagac auccgaggaa     1440

cagaugacca uccucacgcg gcugggaaaa cagaagacaa ccucuucuuc caacaagacc     1500

aaauauauug augagaaacu ucugacagaa gagaucuaca aucccguggu ugccaagagc     1560

gugagacagg cuauuaagau cgucaacgcc gccauuaaag aauauggcga cuucgauaau     1620

aucgugaucg agauggcucg cgaaaccaac gaggacgacg agaagaaagc cauucaaaag     1680

auccagaagg cgaauaaaga ugaaaaggac gcugccaugc ucaaagccgc uaaccaguac     1740

aacgggaagg ccgagcugcc ucacucuguu uuucauggac acaaacagcu ggcuacaaag     1800

auucggcuuu ggcaccaaca gggcgaaaga ugccucuaua cggggaagac caucagcauu     1860

caugaucuga ucaauaacuc caaucaguuc gagguggacc acauccugcc ccucucuauu     1920

acauuugaug acagccuggc caacaaaguc cuuguguacg cgaccgccaa ucaggaaaag     1980

ggccaacgga caccguauca ggcucuggau uccauggacg augccuggag cuuccgcgag     2040

cucaaggcuu uugucagaga gucuaagacc cuguccaaca aaaagaagga auaccugcuu     2100

acggaggaag acaucagcaa auucgaugug cggaagaaau uuauugagag aaaucucguu     2160

gacacccgcu acgccucucg ggugguccug aacgcgcugc aggaacauuu cagagcccac     2220

aagaucgaua caaaaguguc cguugugcgc ggacaauuua ccagccagcu cagacggcau     2280

uggggcauug agaagacacg cgacaccuau caccaccaug cugucgacgc ccugaucauc     2340

gcugccuccu cucagcuuaa ucuguggaag aaacagaaga acacgcucgu gagcuacucc     2400

gaggaucaac ugcuggacau ugaaacaggg gagcuuaucu cugaugacga auauaaagag     2460

agcguguuca aggccccuua ccagcacuuc guugauaccc ucaaauccaa ggaguuugaa     2520

gacagcauuc uguucucuua ucaggucgau uccaaauuua auagaaagau cagcgacgcu     2580

acaauuuacg cgacccggca ggccaaggug ggaaaagaua aggcugacga gaccuaugug     2640

cugggcaaaa ucaaggauau cuacacacaa gacggguacg augccuucau gaaaauuuau     2700

aagaaggaca aaucuaaguu ucucauguac cgccaugacc cccagacguu cgaaaaaguc     2760

aucgagccua uucuggaaaa cuaucccaau aagcagauca acgagaaagg aaaggaggug     2820

cccuguaauc cuuuucuuaa guacaaagag gaacacggcu auauuagaaa guacuccaaa     2880

aaggggaacg gcccggaaau caaaagccug aaguauuacg auucuaagcu cggaaaccac     2940

auugacauca cccccaaaga uagcaauaac aagguugugc ugcaguccgu cagcccuugg     3000

cgggccgacg uguacuucaa uaaaacaacc ggcaaguaug agauccuggg gcuuaaauac     3060

gcugaucucc aauuugaaaa gggaacaggc accuauaaga uuucucagga gaaauacaac     3120

gacaucaaga aaaaggaggg gguugauucc gacagcgagu ucaaauuuac gcuguauaag     3180

aaugaucugc uccuggugaa agacacagaa accaaggaac agcaacuuuu ccgcuuucug     3240

uccagaacca ugcccaagca gaaacauuac gucgagcuca agccuuauga uaaacagaag     3300

uucgaaggag gcgaggcccu gauuaaagug cuggggaacg uugcgaauag cggccagugc     3360

aagaagggac uuggcaaaag caacaucucc auuuacaagg ugcggacaga cguccugggg     3420

aaucaacaca ucaucaaaaa cgagggagac aagcccaaac ucgauuucaa gcgcccggcu     3480

gccaccaaga aagccggcca ggcuaagaaa aagaaauaag gacuagugca ucacauuuaa     3540

aagcaucuca gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau     3600

cucuuuuucu uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu     3660

aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3780

aaaagaauu                                                             3789


<210>  4744
<211>  4347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4744
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccac ccaguucgag      120

ggauuuacaa accuguacca aguuagcaag acgcuccggu ucgaacugau uccgcagggc      180

aaaacccuua agcauaucca ggagcagggg uuuauugaag aggacaaggc gcgcaaugau      240

cacuauaaag aacugaagcc caucauugac agaaucuaca aaacauaugc ugaucaaugc      300

cuccagcugg ugcagcuuga cugggagaac cuguccgccg ccaucgauuc uuaccggaag      360

gagaaaaccg aagagacacg caaugcucuc auugaagaac aggccaccua uagaaacgcu      420

auccaugacu acuucauugg acggacagau aaucugaccg acgccaucaa caagcgccac      480

gcggagauuu auaagggccu guuuaaagcc gaacucuuca augggaaggu gcugaaacaa      540

cuuggaacgg ucaccacaac cgagcaugaa aacgcucugc ucagauccuu ugauaaguuc      600

acaaccuacu uuagcggcuu cuacgagaau cggaaaaacg uguuuucugc cgaagacauc      660

agcacggcua uuccucaccg caucguucag gauaauuucc ccaaguuuaa agagaacugu      720

cacaucuuca caagacugau uaccgccgug ccuucccuuc gggagcauuu cgaaaauguc      780

aagaaggcga ucggcauuuu ugugucuaca agcaucgagg aaguuuucuc cuuucccuuc      840

uauaaccagc ugcucaccca gacccaaauu gaccuguaca aucagcugcu cgggggaauc      900

agccgcgagg ccggcacaga gaaaaucaag gggcugaacg aggugcuuaa ccuggcuauu      960

cagaaaaaug augagacggc ccacaucauu gcuucucucc cucauagauu uaucccgcug     1020

uucaagcaaa uucuuuccga ccggaacacc cugagcuuua uccucgaaga guucaaaucu     1080

gacgaagagg ucauccaguc cuuuugcaag uauaagacac ugcugcgcaa ugaaaacgug     1140

cucgagaccg ccgaggcccu guucaaugaa cuuaacagca uugaucugac acacaucuuu     1200

auuucucaca aaaagcucga gaccaucucc agcgcucugu gugaccauug ggauacgcuu     1260

agaaaugcgc uguacgaacg gagaauuucc gagcucacag gaaaaaucac caagucugcc     1320

aaagagaagg ugcagcgcag ccugaagcac gaagacauua accugcagga gaucaucucc     1380

gcugccggca aagaacucag cgaggccuuc aagcagaaaa ccucugaaau ucugucccau     1440

gcucacgccg cgcuugauca accccugccu acaacccuca agaaacagga ggagaaggaa     1500

auccuuaaga gccagcugga cucucuucuc gggcuguauc aucugcucga uugguuugcu     1560

gucgacgaga gcaaugaagu ugaucccgag uucuccgccc ggcugacagg aauuaaacuu     1620

gaaauggagc cuucucugag cuucuacaac aaggccagaa auuaugcuac gaaaaagccc     1680

uacuccgugg agaaauuuaa gcucaacuuc caaaugccga cccuggccag cggcugggac     1740

gugaauaaag aaaagaacaa uggcgcuauc cuguuuguca agaacgggcu uuacuaucuc     1800

ggaauuaugc cuaaacagaa gggccgcuac aaagcccugu cuuucgagcc cacagaaaag     1860

accuccgagg gguuugauaa aauguauuac gacuauuucc cugacgcggc caagaugauc     1920

cccaagugca gcacacagcu gaaagcugug accgcccacu uucagacgca caccacaccc     1980

auccuccugu cuaacaauuu cauugaaccu cuugagauca ccaaggagau uuacgaucug     2040

aacaauccgg aaaaagagcc caagaaauuu caaacagcuu augccaagaa gaccggagac     2100

cagaaaggcu accgggaagc gcucuguaag uggaucgauu ucacgagaga cuuucugucc     2160

aaauacacaa agaccacaag cauugaucug uccucucuuc gcccuagcuc ccaguauaaa     2220

gaccucgggg aguacuaugc cgagcugaac ccccugcucu accauaucuc uuuccagcgg     2280

aucgcugaaa aggagauuau ggaugccguu gaaaccggaa agcuguaucu uuuucaaauc     2340

uacaauaaag acuucgcuaa gggccaccau ggcaaaccua accugcacac ccucuauugg     2400

acagggcugu ucagccccga gaaucuggcc aagacgucca uuaaacuuaa cggacaggcc     2460

gaacucuuuu acagaccuaa gagccgcaug aaacggaugg cucacagacu gggcgagaag     2520

augcugaaua agaaacucaa ggaucagaaa accccgaucc ccgacacacu guaccaagag     2580

cuuuaugauu acgugaacca ucgccugucu cacgaccucu ccgaugaagc gcgggcccug     2640

cugccuaaug ucauuaccaa ggaggugagc caugaaauca ucaaagacag acgguucaca     2700

ucugacaagu uuuucuuuca cguucccauu acccuuaacu aucaggcugc caauuccccu     2760

agcaaguuca accagcgcgu gaaugccuac cugaaagagc aucccgaaac gccgaucauu     2820

gggaucgaua gaggagagcg gaaccucauu uauaucacag ucauugacuc uaccggcaag     2880

auccuggagc agagaagccu caacaccauc caacaguuug auuaccagaa aaagcuggac     2940

aaucgcgaaa aagagcgggu ggcugccaga caggcguggu ccgucguggg gacaauuaag     3000

gaucuuaagc aaggcuaucu gagccagguu auccacgaaa uuguggaccu caugauccac     3060

uaccaggcug ucgugguucu ggagaaccug aauuucggau uuaaaucuaa gcgcaccggc     3120

auugccgaaa aagccgugua ccagcaauuc gagaagaugc uuaucgauaa acugaacugc     3180

cucguccuga aggacuaucc ugcugagaag guugggggag ugcucaaucc cuaccagcug     3240

acagaucagu uuacguccuu cgccaaaaug ggcacccaaa gcggguuccu guuuuaugug     3300

cccgcgccuu acacauccaa gaucgacccg cugaccggau ucgucgaucc cuuugugugg     3360

aaaacaauua agaaccauga aucuagaaaa cacuuccucg agggcuuuga cuuccugcau     3420

uaugauguga agaccgggga cuuuauccug cacuucaaga ugaaucggaa ccuuagcuuu     3480

cagcgcggcc ugccuggauu caugcccgcu ugggacauug ucuuugagaa aaaugaaacg     3540

caguucgaug ccaagggcac cccuuuuauc gccgggaaaa gaauugugcc cguuaucgag     3600

aaccaucggu ucacaggacg cuacagagac cucuauccug cuaaugaacu gaucgcccuc     3660

cuggaggaaa agggcauugu guuccgggau ggguccaaca uccuucccaa acugcuggag     3720

aaugacgauu cucacgcuau ugacaccaug gucgcccuca uccgcagcgu gcugcagaug     3780

agaaacucca augcggccac aggagaagau uacauuaacu cuccgguucg ggaccuuaac     3840

ggcguguguu uugauagccg cuuccaaaau ccugaguggc ccauggacgc ugaugccaac     3900

ggggcuuacc acaucgcccu gaagggccag cuccugcuca aucaucugaa agaguccaag     3960

gaccuuaagc ugcagaacgg aauuagcaau caggacuggc uggccuauau ccaagaacuc     4020

agaaacaaac ggccugcugc gaccaagaaa gccggccagg cuaagaaaaa gaaguaagga     4080

cuagugcauc acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa     4140

gaucaauagc uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua     4200

aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa     4260

uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aagaauu                                         4347


<210>  4745
<211>  4326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4745
gggagaggcg cugccuacgg agguggcagc caucuccuuc ucggcaucaa gcuuaccaug       60

gcccccaaga aaaagagaaa agugggcauc cacggggucc cugcugccag cauuuaccag      120

gaguucguua acaaguauuc ccugucuaaa acccuccggu uugaacugau cccgcaagga      180

aagacacuug agaauauuaa ggcgcgcggc cugauccucg acgaugaaaa gagagcuaag      240

gacuacaaaa aggccaaaca gauuaucgau aaguaucauc aguucuuuau cgaggaaauu      300

cugagcuccg ugugcaucuc ugaggaccuu cugcagaacu acagcgaugu guauuucaag      360

cucaaaaagu ccgacgauga caaucugcaa aaagauuuua agucugccaa agacacgauu      420

aagaaacaga ucagcgagua cauuaaggau uccgaaaagu ucaaaaaccu guuuaaucag      480

aaccucaucg acgcuaagaa agggcaggag agcgaucuga uucuuuggcu gaagcaaucu      540

aaagacaaug gaaucgaacu cuucaaggcc aacuccgaca ucaccgauau ugacgaggcu      600

cuggagauca uuaagagcuu uaaaggcugg acaaccuauu ucaagggguu ucacgaaaau      660

cggaaaaacg ucuacucuuc caaugauauc cccacaagca uuaucuaccg caucguggac      720

gauaaccuuc cuaaguuccu ggagaauaaa gccaaguaug aaucucucaa ggacaaagcg      780

cccgaggcca uuaacuacga acagaucaag aaagaucugg cugaggagcu gaccuuugac      840

auugauuaua agacauccga aguuaaucag agaguguuca gccucgacga ggucuucgaa      900

aucgccaacu uuaacaauua ccugaaccag uccggaauua ccaaauucaa uacgaucauc      960

ggcggcaagu uugugaacgg ggagaauacc aagcggaaag gaauuaacga auauaucaau     1020

cuuuacucuc aacagauuaa cgauaagaca cugaaaaagu auaaaaugag cguucucuuc     1080

aagcagaucc uguccgacac cgagagcaaa ucuuuuguga uugacaaacu ugaggaugac     1140

uccgaugucg ugacaaccau gcagagcuuc uacgaacaaa ucgcugccuu uaagacggug     1200

gaggaaaaau cuauuaagga gacacugagc cuccuguucg acgaucugaa agcgcagaag     1260

cucgaccugu ccaaaaucua cuuuaagaau gauaagucuc uuaccgaccu gagccagcaa     1320

gucuucgaug acuauuccgu uaucggcaca gccgugcucg aauacauuac ccagcagauc     1380

gcuccuaaaa accuggauaa ucccagcaag aaagagcagg agcuuauugc caagaaaacc     1440

gaaaaggcua aguaucuguc ucucgagaca aucaaacugg cccuggaaga guuuaacaag     1500

caucgcgaca uugauaaaca auguagauuc gaggaaaucc ucgccaauuu ugcugcgauc     1560

ccuaugauuu ucgacgagau cgcccagaac aaggacaauc uggcucagau uuccaucaaa     1620

uaccagaacc aagggaagaa ggaucuucug caggccagcg ccgaagacga ugugaaagcu     1680

auuaaggacc uccuggauca gacgaacaau cugcuucaca aacucaagau cuuccauauc     1740

ucucaguccg aggacaaagc caacauucug gauaaggacg aacacuuuua ucuggucuuc     1800

gaggagugcu acuuugaacu cgcgaauauc gugccgcugu auaacaaaau ucggaauuac     1860

aucacccaaa agcccuacag cgaugagaag uucaaacuua acuuugaaaa uuccacacug     1920

gcuaacggau gggacaagaa uaaagagccu gauaacaccg ccauucucuu caucaaggac     1980

gauaaauauu accugggcgu uaugaauaag aagaacaaua aaaucuuuga cgacaaggcc     2040

auuaaagaaa acaaggggga gggauauaaa aagaucgugu acaagcugcu ucccggcgcu     2100

aauaaaaugc ucccuaaggu cuucuuuucu gccaaaagca uuaaguucua uaaccccucc     2160

gaggauaucc ugcgcauuag aaaccacucu acacauacca aaaauggcag cccgcagaag     2220

ggguacgaaa aguuugaguu caacaucgaa gacugucgga aauuuauuga uuucuauaag     2280

caguccauca gcaaacaccc ugaguggaag gacuucggau uucgcuucuc ugauacgcaa     2340

agauacaauu ccaucgacga auuuuaccgg gagguggaga accagggcua uaaacugaca     2400

uucgaaaaua uuagcgaguc uuacaucgau uccguuguga accaggggaa gcucuaucug     2460

uuucagauuu acaauaagga cuucagcgcu uauucuaaag gacgccccaa ccuucauacc     2520

cuguacugga aggcccucuu ugaugaaaga aaucugcaag acgucgugua uaaacugaac     2580

ggcgaggcgg agcuuuucua ccggaagcag agcaucccua aaaagauuac ccaccccgcc     2640

aaagaagcua ucgccaauaa gaacaaggau aaucccaaaa aggaguccgu cuuugaauac     2700

gaccucauca aagacaagag auucacagag gauaaauuuu ucuuccacug cccuauuacc     2760

aucaacuuua agagcucugg ggcuaauaag uucaacgacg aaauuaaccu gcugcucaaa     2820

gagaaggcca augaugugca uauccugucc auugaccgcg gagagcggca ccuugcguau     2880

uacacacugg uugauggcaa aggcaacauc aucaagcagg acacguuuaa uauuaucggg     2940

aacgauagaa ugaaaaccaa uuaucaugac aagcucgccg cuauugaaaa ggaucgcgac     3000

agcgcccgga aagauuggaa gaaaaucaac aauauuaagg agaugaaaga aggauaccug     3060

ucccaggugg uccacgagau cgcuaagcug gugauugaau auaacgccau cguuguguuc     3120

gaggaccuua auuuuggcuu caagagaggg cgcuuuaaag ucgagaagca aguguaccag     3180

aaacucgaaa agaugcugau cgagaaacug aacuaucucg uguucaagga uaaugaauuu     3240

gacaaaacag gaggcguucu gcgggccuac cagcuuaccg cuccguucga gacauuuaag     3300

aagaugggga aacagaccgg cauuaucuac uauguccccg cgggauucac gucuaagauu     3360

uguccuguga ccggcuuugu gaaccaacug uaccccaaau augaaagcgu cuccaagucu     3420

caggaguucu ucagcaaauu ugacaagauc ugcuacaauc ucgauaaggg guauuucgag     3480

uuuuccuucg acuacaaaaa cuuuggagau aaggccgcua aaggcaagug gacaauugcc     3540

ucuuucggga gcagacugau caauuuucgc aacuccgaca aaaaucauaa cugggauacc     3600

cgggaagugu auccuacaaa ggagcuggaa aaacuucuga aagacuacag caucgaguac     3660

ggacacggcg aguguauuaa ggccgcuauc ugcggggaau cugauaaaaa guucuuugcc     3720

aaacucaccu ccguucugaa cacgauucuc cagaugagaa auagcaagac aggcaccgag     3780

cuggacuauc uuaucucucc cguggcggau gucaacggaa auuucuuuga cucccgccaa     3840

gcuccgaaga acaugccuca ggaugccgac gccaauggcg cuuaccacau ugggcugaaa     3900

ggacucaugc ugcugggccg gaucaagaac aaucaggaag ggaaaaagcu gaaccuugug     3960

aucaaaaaug aggaauauuu cgaguuuguu cagaacagaa auaacaagcg gcccgccgcg     4020

acaaagaagg cuggacaagc caagaaaaag aaauaaggac uagugcauca cauuuaaaag     4080

caucucagcc uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc     4140

uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau     4200

cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

agaauu                                                                4326


<210>  4746
<211>  4582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4746
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccgacaag aaauacagca      120

uuggacugga uaucggcacc aacuccguug ggugggcggu gauuacagac gaguauaagg      180

ugccgucuaa gaaauucaag guccucggaa auacggaucg gcauagcauc aaaaagaacc      240

ugauuggcgc ucugcuguuu gacuccgggg aaaccgccga ggccacacgc cucaaaagaa      300

ccgcucggcg cagauacaca cggcgcaaga auagaaucug cuaucugcag gaaaucuucu      360

cuaacgagau ggccaaggug gaugacagcu uuuuccaccg gcuugaagag uccuuucugg      420

uugaggaaga uaaaaagcau gagcgccacc ccauuuucgg aaauaucgug gacgaagucg      480

cuuaccauga gaaauauccu accauuuacc accucagaaa gaaacuggug gauucuacag      540

acaaggccga ucugcggcuc aucuaucugg cgcuugccca caugauuaaa uuucgcggcc      600

auuuccugau cgagggcgac cucaaccccg auaauagcga cguugacaag cuguuuauuc      660

aacuugugca gaccuacaac cagcuguucg aagagaaucc uaucaacgcu uccggggucg      720

augccaaggc uauccucagc gccagacugu cuaaaucccg gcgccuggaa aaucucauug      780

cgcagcugcc cggagagaag aaaaacggcc uuuuugggaa ucugaucgcc cucagccugg      840

gacuuacgcc uaacuucaag ucuaauuuug accuggcuga agaugccaaa cuccaacugu      900

ccaaggacac cuacgaugac gaucuggaca accuccuggc ucagauuggc gaucaguaug      960

ccgaccuuuu ccuggccgcu aagaaccuca gcgaugcgau ccugcuuucu gacauucuga     1020

gagugaauac agagaucacc aaagccccgc ucuccgcuag caugaucaag cgguacgacg     1080

agcaccauca ggaucugaca cugcucaaag cccuggugag acaacagcuu cccgaaaagu     1140

auaaagagau uuucuuugac caguccaaga acggguacgc cggauauauc gauggcggcg     1200

cuucucagga agaguucuac aaguuuauua aaccuauccu ggaaaagaug gacgggaccg     1260

aggagcuccu ggucaaacug aaucgcgaag aucuucuccg gaagcaaaga acguucgaca     1320

acggaagcau uccccaccag auccaccugg gcgagcugca ugccauccuc cgccggcagg     1380

aagauuuuua uccuuuccug aaagacaaua gagagaagau ugaaaagauc cuuacauuuc     1440

gcauucccua cuacguuggg ccgcuggcgc ggggaaacuc cagauucgcu uggaugaccc     1500

gcaaaagcga ggagacaauc accccuugga auuuugaaga ggugguggau aagggcgccu     1560

cugcccaauc cuucauugaa cggaugacca acuuugacaa aaaucucccc aacgagaagg     1620

uccugccuaa acacagccug cuuuaugagu acuucacagu guauaaugaa cucacgaagg     1680

uuaaauacgu gaccgagggg augagaaagc ccgcuuuucu gucuggagaa cagaagaaag     1740

ccaucgucga ucuucucuuc aagacaaacc gcaaagugac cguuaagcag cugaaagagg     1800

acuauuucaa gaagauugaa uguuuugaua gcguggagau cuccggcguc gaggaccggu     1860

ucaaugcuuc ucuuggcaca uaccaugacc ugcucaaaau cauuaaggau aaagacuuuc     1920

uggauaacga agagaaugaa gacauccugg aggauauugu gcuuacccuc acgcuguucg     1980

aagacagaga gaugaucgag gaacggcuga agacauaugc ccaccucuuu gaugacaaag     2040

ucaugaagca gcugaagcgc agacgguaca ccgggugggg aagacuuagc cgcaaacuga     2100

uuaacggcau ccgggauaag caauccggga aaaccauccu cgacuuccuu aagagcgaug     2160

gauuugcgaa cagaaauuuc augcagcuga uucaugacga uucucugaca uuuaaagagg     2220

acauccagaa ggcccaggug uccggccaag gggacagccu ccacgaacac auugcuaacc     2280

uggccggcuc ucccgcuauc aagaaaggca uucugcagac cguuaaggug gucgaugagc     2340

ucgugaaagu uaugggacgc cauaagccug aaaauaucgu gaucgagaug gccagagaga     2400

accagacaac gcagaaaggg caaaagaacu cccgggaacg caugaaaaga auugaggaag     2460

gaaucaagga gcugggcagc cagauucuua aggagcaccc ggucgaaaau acccagcugc     2520

agaaugagaa acucuaccug uauuaccugc aaaacgggcg ggacauguau guggaucagg     2580

aacuugacau caaucgccug uccgauuacg acguggauca uauuguuccc cagucuuucc     2640

ucaaggacga uagcaucgac aacaaagucc ugacaagauc cgauaagaau cggggaaaau     2700

cugacaacgu gccuagcgag gaagugguca agaagaugaa aaauuauugg cgccaacucc     2760

ugaacgcgaa acuuaucacc cagagaaaau uugacaaucu gacaaaggcc gagcggggcg     2820

ggcucuccga gcuggauaaa gcuggcuuca uuaagcgcca gcugguggaa accagacaga     2880

ucacgaagca cguugcccaa auucuugaca gccggaugaa caccaaauac gaugagaacg     2940

acaagcugau cagagaagug aaagucauua cacucaaguc uaaacuggug uccgauuuuc     3000

gcaaggacuu ccaguucuau aagguucggg agaucaauaa cuaccaccau gcucacgaug     3060

ccuaccucaa ugccgugguc ggaaccgcuc ugauuaaaaa guaucccaaa cuugaaagcg     3120

aguuugugua cggcgacuau aagguuuacg augugagaaa aaugaucgcg aagucugagc     3180

aggaaaucgg gaaggccaca gcuaaauauu ucuuuuacuc caacauuaug aauuucuuua     3240

agaccgagau cacgcuggcc aacggagaaa uucgcaaacg gccucucauc gagacaaaug     3300

gcgaaaccgg ggagauuguc ugggacaagg gaagagauuu cgccacagug cgcaaagugc     3360

ugagcaugcc ccaggucaac aucguuaaga aaaccgaggu gcaaaccggc ggguuuucua     3420

aggaaagcau ccugccgaag agaaauuccg acaaacuuau ugcucggaag aaagauuggg     3480

acccuaagaa auauggcgga uucgacagcc ccacaguggc cuacucuguc cuggugguug     3540

cgaaggugga gaagggcaaa uccaagaaac ucaagagcgu caaagaacug cucgggauca     3600

cgauuaugga gcgcuccucu uuugagaaga acccuaucga uuuccuggaa gcuaagggau     3660

acaaagaggu gaagaaagac cuuauuauca agcugcccaa auauagccug uuugaacucg     3720

agaauggccg gaagagaaug cuggccuccg ccggggaacu ucagaaggga aacgagcugg     3780

cucucccuuc uaaauacguu aauuuccugu aucucgccag ccauuacgag aagcugaaag     3840

gcucccccga agauaacgag cagaagcagc uuuucgugga acaacacaaa cauuaucugg     3900

acgagaucau ugaacagauc ucugaguuua gcaagcgggu cauucuggcg gaugcuaauc     3960

ucgacaaagu gcuguccgcc uacaacaagc accgcgauaa gccgaucaga gagcaggccg     4020

aaaacauuau ccaccuuuuc acccugacaa aucucggggc uccugccgcu uuuaaauauu     4080

ucgacaccac aauugaucgg aagcgcuaca ccagcacgaa agagguucug gacgccacac     4140

ucauccauca aucuaucacc ggccuguacg aaaccagaau ugaucuuucc cagcugggag     4200

gcgacaagcg gcccgcggcc acaaagaagg cugggcaggc caagaaaaag aaaggacuag     4260

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     4320

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     4380

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     4440

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     4560

ucuuuucaga gccaccagaa uu                                              4582


<210>  4747
<211>  5365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4747
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaacuuc aagauucugc      120

cgaucgcgau ugaccucgga guuaaaaaua caggcguguu uagcgcuuuc uaccagaagg      180

ggacaucccu ggagcggcuu gauaacaaga auggaaaagu guaugaacug ucuaaggaca      240

gcuacacgcu ccugaugaac aaucgcaccg ccagacggca ucaacgcaga ggcaucgauc      300

ggaaacagcu ugucaagcgc cuguuuaaac ucauuuggac agagcagcug aaccuggaau      360

gggacaagga uacccagcaa gccaucuccu uccucuuuaa uagacggggg uucucuuuua      420

ucacagacgg auauagcccc gaguaccuga acauugugcc ugaacagguu aaggcuaucc      480

uuauggauau uuucgacgau uauaauggcg aggacgaucu ggacuccuac cucaaacugg      540

ccaccgagca ggaaucuaag aucagcgaga uuuauaacaa acuuaugcag aagauccugg      600

aauuuaaacu caugaagcug ugcacagaua uuaaagacga caaggugucc accaagacgc      660

ugaaagagau caccagcuac gaguucgaac uccuggcuga uuaccuugcc aauuauucug      720

agucccugaa gacacaaaaa uuuagcuaca ccgacaagca gggcaaccuc aaagaacugu      780

cuuauuacca ccaugauaag uauaauaucc aggaguuccu uaagcgccac gcgacaauua      840

acgacagaau ccuggauacc cuccugacgg acgaucugga cauuuggaau uuuaacuucg      900

aaaaauucga uuuugacaag aacgaggaga aacuccagaa ucaagaagau aaggaccaua      960

uccaggccca ucugcaccac uucgucuuug cugugaacaa aauuaagucc gagauggcca     1020

gcgggggacg gcaccgcucu caguacuucc aagaaaucac aaauguucuu gacgagaaca     1080

aucaucagga aggcuaucug aagaacuuuu gugagaaucu ccacaacaaa aaguacucca     1140

aucugagcgu gaaaaaccuu gucaaucuga ucgggaaccu cuccaaucug gagcugaagc     1200

cccucagaaa auacuucaac gauaagauuc acgcuaaggc cgaccauugg gaugaacaga     1260

aauuuaccga gacauauugc cacuggaucc ugggagaaug gcgggugggc gugaaggacc     1320

aggauaaaaa ggacggggcg aaauacucuu auaaggaucu uuguaaugag cugaaacaaa     1380

aggucaccaa ggccggacuc guugacuucc ugcuggaacu ugauccuugc cgcaccauuc     1440

ccccuuaccu cgacaacaac aauagaaaac cgcccaagug ucagagccug auccugaacc     1500

cuaaauuucu cgauaaucag uaucccaacu ggcagcaaua ccugcaggag cuuaagaaac     1560

ugcaguccau ucagaauuau cucgacagcu ucgagacaga ucugaaggug cugaagucuu     1620

ccaaagacca accuuacuuu guggaauaca agagcucuaa ccagcagauc gcuagcggcc     1680

agcgggacua uaaagaucuu gacgccagaa uccuccaauu cauuuuugau cgcgucaagg     1740

cuuccgacga gcugcugcuc aaugaaaucu acuuccaggc caaaaagcug aagcagaaag     1800

ccucuagcga gcuugagaag cuggaaucca gcaaaaagcu cgaugaggug auugcuaacu     1860

cucaacuguc ccagauccug aaaagccagc auacgaaugg cauuuucgaa caggggaccu     1920

uucuucaccu cguuugcaag uauuacaagc aacggcagag agcgcgcgac ucucggcugu     1980

auaucaugcc cgaguacaga uaugauaaaa agcugcauaa auacaacaau acaggacgcu     2040

ucgacgauga caaccagcuc cugaccuacu guaaucacaa gccgcggcag aaaagauauc     2100

aacuucugaa cgaucucgcc ggcgugcugc aggucucccc uaauuuucug aaggacaaaa     2160

uugggagcga ugacgaccuu uucaucucca aguggcucgu ggaacacauc cgcggauuua     2220

agaaggcuug cgaggauucu cugaaaauuc agaaagacaa ccggggccug cucaaccaua     2280

agaucaauau ugccagaaac acaaaaggga agugugagaa ggaaaucuuc aaucugauuu     2340

gcaaaaucga gggaagcgaa gauaagaaag gcaacuacaa gcacggccuu gccuaugagc     2400

uggggguucu ccuguuugga gaacccaaug aggcuuccaa accugaguuc gaccgcaaga     2460

ucaagaaauu uaacucuauu uacagcuucg cccagaucca acagauugcg uuugcugaac     2520

ggaagggcaa ugccaacacc ugugccgugu gcuccgcuga uaaugcccau agaaugcagc     2580

aaaucaaaau uacggagccc gucgaagaca acaaggauaa aaucauccug agcgcuaagg     2640

cccagcggcu ucccgcgauu ccuacacgca ucguggacgg ggccgucaag aaaauggcua     2700

ccauucuggc caagaauauc guggaugaca acuggcagaa uauuaaacag guucucucug     2760

cuaagcacca acugcacauc ccgaucauua ccgaguccaa cgccuucgaa uuugagcccg     2820

cgcucgccga ugugaaagga aagagccuga aggacagacg gaaaaaggcu cuugagagaa     2880

ucucuccuga aaauauuuuc aaagacaaga acaaccgcau caaagaguuc gccaagggca     2940

uuuccgcuua uagcggggcc aaucugacag auggcgacuu ugauggagcc aaagaggaac     3000

ucgaccauau cauuccccgg ucucacaaga aguacggcac ccugaacgau gaggcuaauc     3060

ugaucugcgu cacaagaggg gacaacaaaa auaagggaaa ccgcaucuuc ugucuuagag     3120

aucuggcgga caauuauaaa cucaagcagu uugaaacgac cgaugaccug gagauugaga     3180

aaaagaucgc cgauacaauu ugggacgcua acaagaaaga uuucaaguuu ggcaauuacc     3240

ggagcuucau caaccucacc ccucaggaac agaaagccuu ucgccaugcc cuguuccuug     3300

cugacgaaaa ucccauuaag caagccguga ucagagcgau caacaaucgg aaccgcacau     3360

uuguuaaugg gacccagaga uacuucgcug aggugcuggc caacaauauu uaucuccggg     3420

ccaaaaagga gaaccugaac acggacaaga ucuccuuuga uuacuucggc auuccgacca     3480

ucggaaaugg gcgcggcauu gcugaaauca gacagcugua ugagaaaguc gacagcgaua     3540

uccaggccua cgcgaaggga gacaaaccuc aggcuucuua uucccaccuu auugaugcca     3600

ugcuggccuu cugcaucgcu gccgacgaac aucggaacga uggcagcauu gggcucgaga     3660

ucgacaagaa uuacucccug uauccccucg auaaaaacac aggagaagug uuuaccaagg     3720

acauuuucuc ucaaaucaag auuacagaua augaguuuag cgacaaaaag cuggugcgca     3780

aaaaggcuau cgagggcuuc aacacccaca gacagaugac gcgggauggg aucuacgccg     3840

aaaauuaccu uccuauucug auccacaaag agcugaacga aguuagaaag ggauauacau     3900

ggaaaaauuc cgaggaaauu aagaucuuua agggcaaaaa guacgacauu cagcaacuca     3960

acaaucuggu cuauugucuu aaauucgugg acaagcccau cucuaucgau auucagauca     4020

gcacccugga ggagcuccgc aacauucuga caaccaauaa caucgcggcc accgcugaau     4080

acuauuacau uaaucucaaa acacagaagc ugcaugagua uuacaucgaa aacuacaaca     4140

cggcccuugg guauaagaaa uacuccaagg agauggaguu ucugcggucu cuggcuuaua     4200

gaagcgaacg cgugaaaauc aaguccauug acgaugucaa acaagugcuc gacaaggaua     4260

gcaauuucau cauuggcaag aucacccugc cuuuuaaaaa ggaguggcag cggcuuuaca     4320

gagaauggca gaacacaacc auuaaagacg auuaugaguu ccugaagucu uucuucaaug     4380

uuaaauccau cacaaagcuc cacaagaaag ugcgcaagga cuuuagccug cccauuucua     4440

ccaacgaagg aaaauuccuc gucaagcgga aaacguggga uaauaacuuc aucuaccaga     4500

uccugaauga cuccgauagc agagccgacg gcacaaagcc guuuauuccu gccuucgaca     4560

ucucuaaaaa cgagauugug gaggcuauca uugauuccuu uaccagcaag aauaucuucu     4620

ggcuucccaa gaacaucgaa cugcaaaaag uugacaauaa gaacauuuuu gcgaucgaua     4680

ccuccaaaug guucgaggug gaaacaccca gcgaccugcg cgauauuggg aucgccacca     4740

uucaguauaa gaucgacaau aacucucggc cuaaagucag agugaagcuc gauuacguua     4800

ucgacgauga cagcaagauu aauuacuuua ugaaccauuc ccugcuuaaa ucucgguauc     4860

cggauaaggu gcuggagauc cucaaacaga gcacaauuau cgaauuugag uccucuggau     4920

uuaacaagac gauuaaagag augcugggca ugaagcucgc ugggaucuac aaugaaacca     4980

gcaacaauaa gcgccccgcc gccaccaaaa aggcuggaca ggccaaaaag aaaaagggac     5040

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     5100

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     5160

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     5220

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     5340

ggcucuuuuc agagccacca gaauu                                           5365


<210>  4748
<211>  3637
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4748
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaagcgg aacuacauuc      120

ugggacucga caucggcauu accagcguug gguauggaau cauugauuac gagacacgcg      180

acgugaucga ugcgggcgug agacuguuca aagaagcuaa ugucgagaac aaugaagggc      240

ggcgcuccaa gagaggagcc cggcgccuua agagacggcg cagacaucgg auccagcgcg      300

ugaaaaagcu gcucuuugac uauaaccugc uuacggauca cucugagcug agcggcauua      360

auccguacga agccagaguu aaaggccucu cccaaaagcu gucugaggag gaguucagcg      420

cugcccugcu ccaucuggcu aaacggcgcg gggugcacaa cgucaaugaa guggaagagg      480

acaccggaaa cgagcuuucc acaaaggaac agaucucuag aaauagcaag gcccuggagg      540

aaaaauaugu ugcggagcuc cagcuggaac ggcuuaagaa agauggcgag gugagagggu      600

ccauuaaccg cuuuaagacc agcgacuacg ucaaagaggc caagcagcug cucaaagugc      660

aaaaggcuua ucaucagcug gaucagucuu ucaucgacac auacauugau cugcucgaaa      720

cccggagaac auacuaugag ggacccggcg aagggucccc uuuuggaugg aaggacauca      780

aagaguggua cgaaaugcug augggccacu gcaccuauuu ccccgaggag cuucgcagcg      840

ugaaguacgc cuauaaugcu gaucuguaca acgcccucaa ugaccugaac aaucuuguca      900

uuacgcggga cgaaaacgag aaacuggaau auuacgagaa guuucagauc aucgaaaacg      960

uuuucaaaca aaagaagaaa ccuacccuca agcagauugc gaaagagauc cuggugaaug     1020

aggaagauau uaagggcuac agagugacau cuaccgggaa acccgaguuu acaaaccuga     1080

aggucuauca cgacaucaag gauauuaccg cccgcaaaga aaucaucgag aaugcugagc     1140

uccuggacca gauugccaag auccuuacga uuuaccaguc cagcgaagau auccaagagg     1200

aacugacaaa ccucaauucu gagcugaccc aggaagagau ugagcagauc uccaaccuua     1260

aaggauauac aggcacccau aaucugagcc ucaaggcuau caaccugauu cuggacgaac     1320

ucuggcacac caaugauaac caaaucgcca uuuucaaucg gcugaaacuu gugccuaaga     1380

agguugaccu gucccagcag aaagagaucc cgacaacgcu cguggaugac uuuauucugu     1440

cucccgucgu gaagcgcagc uucauccagu ccauuaaagu uaucaacgcc aucauuaaga     1500

aauacgggcu gccuaaugau aucauuaucg aacuugcucg cgagaagaac agcaaagacg     1560

cgcaaaagau gauuaaugaa augcagaagc ggaacagaca gaccaacgag cgcaucgagg     1620

aaaucauucg gacaaccgga aaagagaaug ccaaguaucu caucgagaaa auuaagcugc     1680

augacaugca ggaaggcaaa ugucuguacu cucucgaagc uaucccccug gaggaucuuc     1740

ugaacaaucc uuuuaacuau gagguggacc acauuauccc cagauccguc agcuucgaua     1800

auucuuucaa caauaaggug cucgucaagc aagaagagaa cagcaaaaag gggaaucgga     1860

caccguuuca guaccugucc ucuagcgacu ccaaaaucag cuacgaaacc uucaagaaac     1920

acauucugaa ccuugccaag ggaaagggcc gcaucucuaa aacgaagaaa gaguaucucc     1980

uggaggaaag agauauuaau cgguuuuccg ugcagaagga cuucaucaac agaaaucugg     2040

uugauacacg cuacgccacc cggggccuca ugaaccugcu uagaagcuau uuucgcguga     2100

auaaccugga cgucaaagug aagucuauua acgggggauu caccuccuuu cucagacgga     2160

aguggaaauu caagaaagag cgcaauaagg gcuacaaaca ucacgcugaa gaugcccuga     2220

ucaucgcgaa cgcugacuuu auuuucaagg aguggaaaaa gcuggauaag gccaaaaagg     2280

uuauggaaaa ucagauguuu gaggagaaac aagccgaaag caugccugag aucgaaacag     2340

agcaggaaua uaaggagauu uucaucacac cccaucagau uaaacacauc aaggacuuua     2400

aggauuacaa auauucccau agaguggaca agaaaccuaa ccgggagcuu auuaaugaca     2460

cccucuacuc uacgcgcaag gaugacaaag ggaacacccu gaucgucaau aaccugaaug     2520

gacucuacga uaaggacaac gauaagcuga agaaacuuau caauaaaagc cccgaaaagc     2580

ugcucaugua ucaccacgac ccccagacau accaaaaacu gaagcugauu auggagcagu     2640

auggcgauga aaagaacccu cuuuacaaau auuacgagga aaccgggaau uaucucacaa     2700

aguacuccaa aaaggacaac ggcccgguga ucaaaaagau uaaguacuau ggaaauaaac     2760

ugaacgcuca ucuggauauc accgacgauu accccaacuc uagaaauaag gugguuaaac     2820

ucagccugaa gccuuaucgg uucgacgucu accuugauaa cggcguguau aaauucguga     2880

cggucaagaa ucuggacgug auuaaaaagg agaacuacua ugagguuaau uccaagugcu     2940

acgaagaggc caaaaagcuc aaaaagauca gcaaccaggc ugaauuuauc gccucuuucu     3000

acaauaacga ccugauuaaa aucaaugggg agcuguaucg cgugauugga gucaacaaug     3060

aucuucugaa cagaaucgag gugaauauga uugacaucac cuaccgggaa uaucucgaga     3120

acaugaauga uaagagaccc ccuagaauca uuaagacaau cgcguccaaa acccaaagca     3180

uuaagaaaua cucuacagac auccugggca accucuauga aguuaagucc aaaaagcacc     3240

cccagauuau caagaaaggg aagcggccgg ccgcuaccaa aaaggccgga caggcuaaaa     3300

agaagaaagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     3360

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     3420

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     3480

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     3600

ccccccccca aaggcucuuu ucagagccac cagaauu                              3637


<210>  4749
<211>  4705
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4749
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcccuguuc aacaagugca      120

uuaucauuag caucaaucuc gacuuuucca acaaagagaa guguaugacc aagccguacu      180

cuauuggacu ggauaucggc acaaauagcg uugggugggc ggugaucacg gacaacuaua      240

aagugcccuc caagaaaaug aagguccuug gaaauaccuc uaaaaaguac auuaagaaaa      300

accugcucgg cgugcugcuu uucgauagcg ggaucacagc ugaaggacgg cgccugaaga      360

gaaccgcccg gcgcagauau acacggcgca gaaaucggau ucucuaccug caggagaucu      420

uuuccaccga aauggccaca cuggacgaug cuuucuuuca acgccucgac gauucuuucc      480

ugguuccuga cgauaaaaga gacagcaagu aucccauuuu uggcaaccuu guggaggaaa      540

aagucuacca ugaugaguuc ccuaccaucu aucaucugcg gaaguaccuc gccgacucca      600

cgaaaaaggc ugaucugcgc cuuguguacc uggcccucgc gcacaugauu aaguauagag      660

gccacuuucu gaucgagggg gaguucaaua gcaaaaacaa ugacauccag aagaacuuuc      720

aggacuuccu ggauaccuac aaugccauuu uugaaucuga ccucucccug gaaaacagca      780

aacagcuuga ggagaucguu aaggauaaaa uuucuaagcu ggaaaagaaa gaccggaucc      840

ucaagcuguu ccccggagag aaaaauuccg gcauuuucag cgaauuucuu aagcugaucg      900

uggggaacca agcugauuuc cgcaaaugcu uuaaccucga cgagaaggcc ucucugcauu      960

ucuccaagga aagcuaugau gaggaccugg agacacuccu gggauacauc ggcgaugacu     1020

auuccgaugu cuuucuuaaa gcuaagaaac uguacgacgc cauucuccug ucuggguucc     1080

uuaccgugac agacaaugaa accgaggcgc cucugagcuc cgccaugauc aagagauaua     1140

acgaacacaa agaggaucuc gcucugcuga aggaauacau ucggaauauc agccucaaga     1200

cguacaacga gguguuuaaa gacgauacaa agaauggaua ugccggcuac auugacggca     1260

aaaccaacca ggaggauuuc uaugucuacc ugaagaaucu ucuggcugaa uuugaggggg     1320

ccgacuauuu ccucgaaaag aucgauagag aggacuuucu gcgcaagcag cggacauucg     1380

auaacggauc uaucccguac cagauucauc ugcaagaaau gagagccauc cuugacaaac     1440

aggcuaaguu uuaucccuuc cucgcgaaga auaaagagcg cauugagaag auccugaccu     1500

uccggauucc uuacuacguu ggcccccugg ccagagggaa cuccgauuuu gcuuggagca     1560

uccgcaaacg gaaugaaaag auuaccccuu ggaacuucga ggacgugauc gauaaagaau     1620

cuagcgccga ggccuuuauc aauagaauga cauccuucga ccucuaucug cccgaggaaa     1680

aggugcuucc gaagcacucu cugcucuacg agacguuuaa cgucuauaau gaacugacca     1740

aagugcgcuu cauugcugag agcaugcggg acuaccaguu ucuggauucc aagcagaaaa     1800

aggacaucgu uagacuuuau uucaaagaua agcgcaaggu gacagacaaa gauauuaucg     1860

aauaccucca cgccauuuau ggauacgacg gcaucgagcu gaaggggauc gagaaacaau     1920

uuaacagcuc ucuguccacc uaccaugauc uccugaacau uaucaaugac aaggaguucc     1980

uugaugacag cucuaacgaa gcgauuaucg aagagauuau ccacacacug accaucuuug     2040

aagaucggga gaugauuaaa cagagacucu ccaaguucga gaauaucuuu gacaagagcg     2100

uccugaaaaa gcugucccgg cgccacuaua cgggaugggg caaacuuucu gcuaagcuca     2160

uuaacggcau cagagaugaa aagagcggga auacaauucu ggacuaccug aucgacgaug     2220

gaaucuccaa ccggaauuuc augcagcuca uucaugacga ugcccugucu uucaagaaaa     2280

agauccaaaa ggcccagauu aucggcgacg aggauaaagg gaacauuaag gaagugguua     2340

aaagccuucc uggauccccc gcuaucaaga aaggcauucu gcagagcauc aagaucgugg     2400

acgagcucgu caaggugaug ggcgggagaa aaccugaauc uauugucgug gagauggccc     2460

gcgagaauca guauaccaac caaggaaagu ccaauagcca gcagcggcug aaaagacugg     2520

aaaagucucu uaaagagcuc ggcuccaaga uccugaagga aaacauuccc gcuaaacuga     2580

gcaagaucga uaauaacgcc cuccagaaug accgccugua ccuuuauuac cugcaaaacg     2640

ggaaagauau guauaccgga gacgaucucg acauugacag acugucuaac uacgauaucg     2700

accacaucau uccccaggcg uuucugaagg auaauagcau cgacaacaaa guucuugugu     2760

ccagcgccuc uaaucggggc aaguccgaug acuucccuag ccuggagguc gugaagaaac     2820

gcaagacauu uugguaccag cuccugaaau ccaagcucau uucucagaga aaauucgaua     2880

accugaccaa ggcugagcgc gggggacuuc ugccggaaga caaggccggc uuuauccaac     2940

ggcagcucgu ugagacaaga cagauuacga aacauguggc ucggcugcug gaugaaaagu     3000

ucaauaacaa aaaggacgag aauaaccgcg ccgucagaac cgugaaaauc aucacacuua     3060

agagcacccu ggugucccag uuucggaaag auuucgaacu cuauaagguu cgcgagauua     3120

augacuuuca ccaugcgcac gaugccuacc ugaacgcugu caucgccucu gcucuccuga     3180

agaaauaucc caaacuugag ccugaguucg uguacgggga cuaucccaaa uacaauagcu     3240

uuagagaacg gaaguccgcc acagaaaaag uguauuucua cucuaacauu augaauaucu     3300

ucaaaaagag cauuucccug gccgacggca gagucaucga gcgcccucuc auugaaguga     3360

acgaggagac cggagaaagc guuuggaaua aagagucuga ucuggcuacg gugcggagag     3420

uccuguccua cccccaagug aacguuguga agaaagucga ggaacagaac cacggccuug     3480

accgcgggaa gccgaaagga cuguuuaaug cgaaccucag cucuaagccu aagcccaauu     3540

ccaacgaaaa ucuggugggc gccaaagagu aucucgaucc uaagaaauac gggggauaug     3600

cuggcaucag caacucuuuc gccguucugg ugaaggggac caucgagaaa ggcgccaaga     3660

agaaaauuac aaauguccuu gaauuucagg gaaucuccau ucuggaccgg aucaacuaca     3720

gaaaggauaa acugaauuuc cuccuggaga agggcuauaa agacauugaa cuuaucaucg     3780

agcugcccaa guacagccuc uuugagcugu ccgaugggag ccgccggaug cucgcuucua     3840

uucugagcac caacaauaaa agaggagaaa uccauaaggg caaccaaauu uuccuuuccc     3900

agaaguuugu gaaacugcug uaucacgcca agcgcaucuc uaauacaauu aacgagaauc     3960

aucggaaaua cguggaaaac cacaagaaag aguucgagga acucuuuuac uauauccugg     4020

aguucaauga aaacuacguc ggggcgaaga agaacggaaa acuucugaau agcgcuuuuc     4080

aguccuggca gaaccacucu aucgacgagc ucuguagcuc cuucauuggc ccuaccgggu     4140

cugaaagaaa gggccuguuu gagcucacga gccggggauc cgccgccgau uucgaguucc     4200

ugggcguuaa aaucccccgc uauagagacu acacaccgag cucucuucug aaggaugcua     4260

cccugauuca ucaauccgug acagggcucu augaaacccg gaucgaccug gccaaacuug     4320

gagagggcaa gcgcccugcg gcuaccaaga aagccgggca ggccaagaaa aagaaaggac     4380

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     4440

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     4500

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     4560

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     4680

ggcucuuuuc agagccacca gaauu                                           4705


<210>  4750
<211>  3781
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4750
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccugguac gcgagccuga      120

uguccgcuca ucaccuccgg guuggaauug acgugggcac ccauucugug gggcuggcca      180

cacuucgcgu cgaugaccac ggaacgccga ucgagcugcu cagcgcccug ucccauauuc      240

acgauucugg cguggggaag gaagggaaaa aggaccacga uaccagaaag aaacuuagcg      300

gcaucgcucg gcgcgccaga cggcugcucc aucaccgcag aacacagcug caacagcugg      360

acgagguucu ccgggaucug ggcuucccca uuccuacccc cggggaauuu cuugaccuga      420

acgagcagac agauccuuau cgcgugugga gaguccgggc ucgccucgug gaagagaagc      480

ugcccgagga acuuagagga ccugccaucu ccauggcggu ucggcauauc gcccgccaca      540

gaggcuggcg gaauccguac ucuaaagugg agagccugcu cucccccgcu gaagagagcc      600

cuuucaugaa ggcccugaga gagcgcauuc uggcuaccac aggggaaguc cucgacgaug      660

gaaucacccc cggccaggcc auggcgcaag uggcccugac gcacaacauu ucuaugcggg      720

ggccugaggg aauccuuggc aaacugcauc aguccgacaa ugcuaacgaa auuagaaaga      780

ucugcgcccg ccagggcgug agccccgaug ucuguaagca gcuccugcgg gcuguuuuua      840

aagccgacuc uccgagaggg uccgccguga gccgcguggc uccugacccc cuuccuggac      900

aaggcucuuu ccggagagcg cccaagugcg aucccgaguu ucagcgcuuc cggauuaucu      960

ccaucgucgc caaucugaga auuagcgaaa ccaaagggga gaaccgcccu cucacagcug     1020

acgagcggag acacgugguu accuuucuga cagaagauuc ccaggccgac cugaccuggg     1080

uggaugucgc cgagaagcuc ggagugcauc ggcgcgaccu gagaggcacg gcuguucaca     1140

cagaugacgg ggaacggucu gccgcgagac cgcccaucga ugcuaccgac cgcauuaugc     1200

ggcagacaaa aaucagcucc cuuaagaccu ggugggagga agccgauagc gagcaaagag     1260

gagccaugau ucgcuaucug uacgaggacc cuaccgacuc ugaaugugcu gagaucaucg     1320

ccgaacuccc cgaggaagau caggcuaaac uggacucccu ucaucugccu gccggcagag     1380

cggccuauag ccgggagucu cucacagcuc ugagcgauca caugcuggcc acgaccgacg     1440

aucuccacga ggcucgcaag agacuguucg gcguggacga uuccugggcg cccccggccg     1500

aagccauuaa ugcuccuguc gggaaccccu cuguggaccg gacacuuaag aucgucggac     1560

gcuaccugag cgccguggag uccauguggg gcaccccuga aguuauucau guggagcacg     1620

ucagagaugg guuuacaagc gagcggaugg cugacgaacg cgauaaagcc aauagacggc     1680

gcuauaacga caaucaggag gccaugaaga aaauccaaag agauuacgga aaggaaggcu     1740

acauuucucg gggagacauc gugagacucg acgcucugga gcugcagggc ugcgcguguc     1800

uuuauugcgg aaccacgauc ggcuaccaua caugucagcu cgaucacauu guuccccagg     1860

ccgggccugg auccaacaau cgccggggca accugguggc ugucugcgaa agauguaauc     1920

gcagcaaauc uaacaccccc uucgccgugu gggcccaaaa gugcgggauc ccgcacgugg     1980

gaguuaagga ggcuauuggc cgggucagag gguggcgcaa acagaccccu aacacaucca     2040

gcgaggaccu gacccggcuc aagaaagaag ugaucgccag acugcgccgg acacaggagg     2100

aucccgaaau ugacgagaga uccauggaau cuguggcgug gauggcuaau gagcuucauc     2160

accggaucgc cgccgcuuau cccgagacga ccgucauggu guaccgcggc agcauuacag     2220

ccgcggcuag aaaggccgcc ggaaucgauu cccggaucaa ccugauuggc gaaaaagggc     2280

gcaaggacag aaucgaucgg agacaucacg cuguugacgc cucugugguc gcucucaugg     2340

aggccagcgu ggcgaagacc cuggccgaac gcuccagccu gcggggagag cagagacuua     2400

caggcaaaga acaaaccugg aagcaguaua cggggucuac cguuggagcu cgcgagcauu     2460

uugagaugug gcggggccac augcuccacc ugacagaacu guucaaugag agacucgccg     2520

aagauaaagu guacgucacc cagaacauuc gccugcggcu uuccgacggg aaugcucaua     2580

cagugaaccc uagcaagcug guuucucaca gacucggcga uggacugacc gugcagcaaa     2640

ucgaccgcgc cuguacgccg gcccuguggu gcgcucuuac aagagagaaa gauuuugacg     2700

agaagaaugg ccuccccgcg cgggaagacc gcgccauuag aguccauggg cacgagauca     2760

aguccagcga uuauauccag guguucucua aacggaagaa aaccgacagc gaucgcgacg     2820

aaacaccuuu cggagcuauu gccgugagag gcggguuugu cgagaucgga cccuccauuc     2880

aucacgcccg gaucuacaga guugaaggca agaaaccugu guacgcuaug cugcgcgugu     2940

ucacccacga ucugcucagc cagcggcaug gggaccuguu uucugccguc auucccccuc     3000

aguccaucag caugagaugc gcggagccca aacuucgcaa agcuaucacc acaggcaacg     3060

ccacguaucu gggaugggug guugugggcg augagcucga aauuaauguc gacuccuuca     3120

ccaaguacgc caucgggcgg uuucuggagg auuucccgaa cacaaccaga uggcggauuu     3180

guggauauga cacaaauucu aagcugaccc uuaaaccuau cgugcucgcu gccgaaggcc     3240

uggagaaccc cagcuccgcg guuaaugaaa uuguggagcu gaaggggugg cgcgucgcua     3300

ucaacgugcu cacgaaaguu cacccuacag uggucagacg ggaugcccug ggacgcccca     3360

gauacucuag ccgguccaau cuuccgaccu cuuggaccau cgagaagcgc ccugccgcua     3420

caaagaaggc cggccaagcu aagaaaaaga aaggacuagu gcaucacauu uaaaagcauc     3480

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     3540

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     3600

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     3720

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     3780

u                                                                     3781


<210>  4751
<211>  4642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4751
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaccaag ccguacagca      120

uuggacugga caucggcaca aacuccguug ggugggcggu gacgaccgau aauuauaaag      180

ugcccucuaa gaagaugaaa guccucggaa acacaagcaa gaaauacauu aagaaaaauc      240

ugcuuggcgu gcuccuguuc gacuccggga ucaccgcuga gggacggcgc cugaagagaa      300

cagcccggcg cagauauacc cggcgcagaa accggauucu uuaccugcag gaaaucuuuu      360

cuacagagau ggccacccuc gaugacgcuu ucuuucaacg ccuggaugac agcuuccugg      420

uuccugauga caagagagau uccaaauauc ccaucuuugg caaucucgug gaagagaagg      480

ccuaccauga cgaguucccu acgauuuauc aucugcggaa auaccuugcu gauucuacca      540

agaaagccga ccugcgccuc gucuaccugg cgcuugccca caugaucaag uauagaggcc      600

acuuucugau ugagggggag uucaacagca aaaauaacga uauccagaag aauuuucagg      660

acuuccucga cacauacaac gcuauuuuug aauccgaucu gagccuggaa aauucuaagc      720

agcucgaaga gaucgugaaa gacaagauuu ccaaacugga gaagaaagau cggauccuua      780

agcuguuccc cggagaaaag aacagcggca ucuuuucuga guuccucaaa cugauuguug      840

ggaaucaagc cgacuuccgc aagugcuuua accuugauga aaaggcuucc cugcauuuca      900

gcaaagaguc uuaugacgaa gaucucgaga cccugcuggg auacaucggc gacgauuauu      960

ccgacguguu ucucaaagcc aagaagcugu acgaugcgau ucuucugagc ggguuccuca     1020

cagucaccga caaugagacg gaagccccuc uguccucugc uaugaucaaa agauauaacg     1080

agcacaagga agaccuugcc cugcucaaag aguacauucg gaacaucagc cugaagacau     1140

acaaugaagu guuuaaagau gacaccaaga acggauaugc uggcuacauc gauggcaaga     1200

caaaucagga ggacuucuau guguaccuga aaaagcuccu ggccgaguuu gaaggggccg     1260

auuauuuccu ugagaaaauu gacagagaag auuuucugcg caagcagcgg accuucgaca     1320

acggauccau cccguaccag auucaccucc aagagaugag agcuauccug gauaaacagg     1380

cgaaguuuua ucccuuccug gccaaaaaua aggaacgcau ugagaagauc cuuaccuuuc     1440

ggaucccuua cuacgucggc ccccucgcua gagggaacag cgacuucgcc uggucuauuc     1500

gcaaacggaa ugagaagauc acaccuugga acuucgaaga uguuauugac aaagagucca     1560

gcgccgaggc uuuuaucaau agaaugacgu cuuucgaucu guaucugccc gaagagaagg     1620

ugcucccgaa acauagccug cuuuacgaaa ccuuuaacgu guauaaugag cugacaaagg     1680

uccgcuucau ugccgaaucc augcgggacu accaguuucu cgacucuaag cagaaaaagg     1740

auaucgugag acuguauuuc aaagacaagc gcaaaguuac cgauaaggac auuaucgagu     1800

accugcacgc gaucuaugga uacgauggca uugaacuuaa ggggaucgag aaacaauuua     1860

acagcucccu cagcacauac caugaccugc ugaauauuau caacgauaag gaguuccucg     1920

acgauucuuc caaugaagcu auuaucgagg aaaucauuca cacccugacg aucuuugagg     1980

accgggaaau gauuaaacag agacuuagca aguucgagaa caucuuugau aaaucugugc     2040

ugaagaagcu cucccggcgc cacuauacag gauggggcaa acugagcgcc aagcugauua     2100

acggcaucag agacgagaaa uccgggaaua ccauccuuga uuaccucauu gacgacggaa     2160

ucucuaaccg gaauuucaug cagcugauuc augaugacgc ccugagcuuu aagaaaaaga     2220

uccaaaaagc ucagauuauc ggcgaugaag acaaggggaa caucaaggag gucgugaaau     2280

cccucccugg aucucccgcc auuaagaaag gcauccugca gagcauuaag aucguugaug     2340

aacuugugaa agucaugggc gggagaaagc cugaguccau uguggucgaa auggcucgcg     2400

agaaucagua uaccaaccaa ggaaagagca auucucagca gcggcugaaa agacucgaga     2460

agucccugaa agaacugggc agcaagaucc uuaaagagaa cauucccgcc aagcucucua     2520

agaucgacaa uaacgcgcug cagaaugauc gccuguaccu cuauuaccug caaaacggga     2580

aagacaugua uacaggagau gaccuugaua ucgacagacu guccaauuac gacauugauc     2640

acaucauucc ccaggccuuc cucaaggaca acagcaucga uaauaaagug cugguuucua     2700

gcgcuuccaa ccggggcaag agcgacgaug ugccuucucu ggaagucgug aaaaagcgca     2760

agaccuucug guaccagcuu cugaaaucca agcucauuag ccagagaaaa uuugacaacc     2820

ugacaaaggc cgagcgcggg ggacucuccc cggaggauaa agcuggcuuc auccaacggc     2880

agcugguuga aacgagacag aucaccaagc auguggcccg gcuucuggac gagaaguuua     2940

auaacaaaaa ggaugaaaau aaccgcgcgg ucagaacagu gaaaauuauc acccucaagu     3000

cuacacuggu gagccaguuc cggaaagacu uugagcugua uaagguucgc gaaauuaaug     3060

auuuccacca ugcccacgac gcuuaccuua acgccgucgu ggcuuccgcc cugcucaaaa     3120

aguaucccaa gcuggagccu gaguuugugu acggggauua ucccaaauac aauucuuuca     3180

gagaaagaaa gagcgccacc gagaaagucu auuuuuacuc caacaucaug aauauuuuca     3240

agaaaucuau cagccucgcu gacggccggg ugaucgaacg cccucugauu gagguuaacg     3300

aagagacggg agaguccgug uggaauaagg aaagcgaccu ugcgaccguc cggagagugc     3360

ugucuuaccc ccaaguuaac guggucaaga aaguggagga acagaaucac ggccucgauc     3420

gcgggaagcc gaaaggacug uuuaacgcca aucuguccag caagccuaaa cccaacucua     3480

acgagaaucu uguuggcgcu aaggaauauc uggacccuaa gaaauacggg ggauaugccg     3540

gcaucuccaa cagcuucaca gugcucguca aggggaccau ugagaaaggc gccaagaaaa     3600

agaucacaaa ugugcuggag uuccagggaa uuucuauccu cgaucggauu aacuacagaa     3660

aggacaaacu gaauuuucuu cuggaaaagg gcuauaaaga uaucgagcug aucauugaac     3720

uccccaagua cucccuguuc gagcuuagcg acgggucccg ccggaugcug gcuagcaucc     3780

ucucuaccaa caauaaaaga ggagagauuc auaagggcaa ccaaaucuuu cugagccaga     3840

aauucgugaa gcuccuguau cacgccaagc gcauuuccaa uacgaucaac gaaaaucauc     3900

ggaaauacgu cgagaaccac aagaaagaau uugaggaacu uuucuacuau auccuggagu     3960

uuaaugagaa cuacguuggg gcgaagaaaa auggaaagcu gcucaacucu gcuuuccaga     4020

gcuggcagaa ucacuccauu gaugaacugu guucuagcuu uaucggcccu acaggguccg     4080

agagaaaggg ccuuuucgaa cugaccucuc ggggaagcgc cgccgacuuu gaguuccucg     4140

gcgugaaaau uccccgcuau agagauuaca caccguccag ccugcucaag gacgcuaccc     4200

ugauccauca aucugugacc gggcuuuaug aaacacggau ugaucuggcc aaacugggag     4260

agggcaagcg cccugcggcu acgaaaaagg ccgggcaggc caagaaaaag aaaggacuag     4320

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     4380

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     4440

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     4500

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     4620

ucuuuucaga gccaccagaa uu                                              4642


<210>  4752
<211>  3724
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4752
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccgcggcu uucaagccga      120

acagcauuaa uuacauccug ggacucgaca uuggcaucgc cuccguuggg ugggccaugg      180

uggagauuga ugaagaggaa aaccccaucc ggcugaucga ccuuggagug cgcgucuuug      240

agagagcuga agugccuaaa accggcgauu cucuggccau ggcucggcgc cucgccagaa      300

gcguucggcg ccugacaaga cggcgcgcgc auagacuucu gcggacgcgc agacuccuga      360

agcgggaggg ggugcugcag gccgcuaauu ucgacgagaa cggacucauu aagucccugc      420

ccaauacccc uuggcaacuu cgcgccgcug cccuggauag aaaacucaca ccccuggaau      480

ggucugcggu ccuucugcac cucaucaagc aucggggcua ucugagccag cgcaaaaacg      540

agggcgaaac cgccgacaag gagcuggggg cucuccugaa aggaguggcc ggcaaugcuc      600

acgcccuuca gacaggggau uuuagaaccc cugccgagcu ggcucucaac aaguucgaaa      660

aggaguccgg acauauucgg aaucagagau cugacuacag ccacacauuu ucccgcaaag      720

aucugcaagc ggaacuuauc cuccuguucg agaagcagaa agaauuuggc aacccgcacg      780

uuagcggggg acugaaggag ggcauugaga cccugcucau gacgcagcgg cccgcccugu      840

cuggcgacgc ugugcagaaa augcuugggc auugcaccuu cgaaccugcc gagcccaagg      900

ccgcuaaaaa uacauauacc gccgaaagau uuaucuggcu gacaaagcuc aacaaucugc      960

gcauucuuga gcaaggaucc gaacggccuc ugaccgauac ggagagagcg acacucaugg     1020

acgagcccua ccgcaagagc aaacugaccu augcucaggc ccggaagcug cucggccugg     1080

aagauacagc cuucuuuaaa gggcuuagau acggaaagga caacgcugag gccucuaccc     1140

ugauggaaau gaaggcuuau cacgccaucu cccgcgcgcu cgagaaggaa ggccugaagg     1200

acaaaaagag cccgcugaau cuuucuccug agcuccagga ugagaucggg accgccuucu     1260

cccuguuuaa aacagacgaa gauauuacgg gacggcugaa ggacagaauc cagcccgaga     1320

uucucgaagc ucugcuuaaa cauaucagcu ucgauaaguu cguccaaauu ucccugaagg     1380

cccuccggcg caucgugccu cugauggagc agggcaaaag auacgacgag gcuugugccg     1440

aaaucuacgg cgaucacuau gggaagaaaa acaccgagga aaagauuuac cugccuccca     1500

uccccgcgga cgagauucgg aacccggugg uccuuagagc ccucucucag gcucgcaaag     1560

uuaucaaugg aguggugcgg agauauggca gccccgcccg cauucacauc gaaacagcua     1620

gagaggucgg gaaguccuuu aaggaucgga aagagaucga aaagcgccaa gaggaaaaca     1680

gaaaagaccg ggagaaggcc gccgcuaaau uccgcgaaua cuuuccuaau uucgugggag     1740

agcccaagag caaagauauu cugaagcuga gacucuauga gcagcagcau ggcaagugcc     1800

uguacucugg gaaagaaauc aaccuuggcc ggcugaauga gaagggauau guugaaauug     1860

accacgcgcu cccuuuuucc cgcaccuggg acgauagcuu caacaauaaa gugcuggucc     1920

ugggcucuga gaaccagaau aaggggaacc aaacacccua cgaauacuuu aauggaaaag     1980

acaacagcag agaguggcag gaguucaagg cccgggugga aaccucccgc uuuccgagau     2040

cuaagaaaca gcggauccuu cuccagaagu ucgaugagga cggcuuuaaa gaaagaaauc     2100

ugaacgauac gcgcuauguu aaucgguucc ugugucaauu uguggcugac agaaugcgcc     2160

ucacagggaa gggcaaaaag cgggucuucg ccagcaacgg acagauuacc aaccugcuua     2220

gaggguucug gggccugcgc aaggugcggg ccgagaauga uagacaucac gcucucgacg     2280

ccgucguggu ugcgugcucc accguggcua ugcagcagaa aaucacacgc uuuguccggu     2340

acaaggagau gaacgccuuc gauggaaaaa ccauugacaa ggaaacaggc gaggugcugc     2400

aucagaaaac gcacuuuccu cagcccuggg aguucuuugc ccaggaaguu augaucagag     2460

uguucgggaa gccugaugga aagcccgaau uugaggaggc ugacacccug gaaaaacuuc     2520

ggacacuccu ggccgagaag cugagcucuc gcccugaagc gguccacgag uaugugacac     2580

cccucuucgu guccagagcu ccgaaucgga aaaugagcgg ccaggggcau auggaaaccg     2640

uuaagucugc caaacgccug gaugagggag ucuccgugcu uagagugccu cugacccaac     2700

ucaagcugaa agaccuggag aagaugguca accgggaaag agagcccaaa cuuuacgaag     2760

cccugaaagc ucgccucgag gcccacaagg acgaucccgc uaaagccuuu gcggaaccuu     2820

ucuauaagua cgacaaagcc ggcaaucgga cgcaacaggu gaaggcuguu agaguggagc     2880

aaguccagaa gaccggggug uggguucgca accauaaugg caucgccgau aacgcuacaa     2940

uggugcgggu cgacguguuu gagaaaggag auaaguauua ccugguuccg auuuacagcu     3000

ggcaaguggc caaaggcauc cuccccgaca gagccgucgu gcaggggaag gaugaagagg     3060

acuggcagcu gauugaugac uccuucaauu uuaaauucuc ucuucacccu aacgaucugg     3120

uggaagucau caccaagaag gcucgcaugu ucggauauuu ugcgagcugu caccggggca     3180

cagggaauau uaacaucaga auccaugacc ucgaucacaa aauuggaaag aauggcaucc     3240

uggaggggau uggcguuaaa accgcccugu ccuuccagaa guaccagauc gacgagcuug     3300

gaaaagaaau ucgccccugc agacugaaga agcggccucc cgugcgcaaa agaccugcug     3360

ccacgaagaa agccggccag gcuaagaaaa agaagggacu agugcaucac auuuaaaagc     3420

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     3480

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     3540

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     3720

aauu                                                                  3724


<210>  4753
<211>  4480
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4753
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaagaaa ccguacacca      120

uuggacugga caucggcaca aacagcguug ggugggcggu gcucacggau caguaugacc      180

uggugaagcg gaagaugaaa auugcuggag auuccgagaa gaaacaaauc aagaaaaauu      240

ucuggggcgu ccgccuuuuu gacgaagggc agaccgccgc cgauagacgg auggcucgca      300

cagccagacg gcgcauugag agacggcgca acagaaucuc uuaccugcag ggaaucuucg      360

cugaagagau gagcaagacc gacgccaauu ucuucugccg gcucuccgau ucuuuuuaug      420

uggacaacga aaagcgcaau agcagacauc ccuucuuugc gacaauugag gaggaaguug      480

aguaccacaa aaacuauccu accaucuacc aucugcggga ggaacuuguc aauuccucug      540

agaaggccga ucugcgccuc gucuaucugg cucuggccca cauuaucaaa uacagaggca      600

acuuccucau ugaaggcgcu cuggacacac agaauaccag cguggauggg aucuacaagc      660

aauuuauuca gacguauaac cagguuuucg ccuccggaau cgaggacggc agccuuaaaa      720

agcuggaaga uaauaaagac guggcgaaga uccucgucga gaaggugacc cggaaagaaa      780

agcuggagcg cauucuuaaa cuguaccccg gggagaaguc ugccggaaug uuugcucagu      840

ucaucucccu cauugugggc agcaaaggga acuuucaaaa gccuuucgac cugaucgaaa      900

agucugauau ugagugugcc aaagacuccu augaagagga ucuggaaagc cuccuggcuc      960

uuaucggaga cgaguacgcc gagcuguucg ucgccgcuaa gaaugcguau ucugccguug     1020

ugcucuccag caucauuaca guggcugaaa ccgagacaaa cgccaaacug uccgccucua     1080

ugaucgaaag auuugauacc caugaggaag accuuggcga gcugaaggcu uucauuaaac     1140

uccaucugcc caagcacuac gaggaaaucu uuagcaauac ggagaagcac ggcuaugccg     1200

gguacauuga uggaaaaaca aagcaggcgg acuucuacaa auauaugaag augacccugg     1260

aaaacaucga gggcgcugau uacuuuaucg ccaaaauuga gaaggaaaac uuccuccgga     1320

agcagagaac auuugacaau ggggccaucc cucaccagcu gcaucuugag gaacuggagg     1380

cuauucucca ccaacaggcc aaauauuacc cguuccugaa ggaaaacuau gauaaaauca     1440

agucccuugu caccuuucgc auucccuacu ucgugggacc ucuggcuaau ggccagagcg     1500

aguuugccug gcucacccgg aaagcggacg gggagaucag acccuggaac auugaagaga     1560

agguugacuu cggaaaaucu gccguggauu uuaucgaaaa gaugacaaau aaggacacgu     1620

aucugccuaa agagaacguc cugcccaagc acucccucug cuaccaaaaa uaccuggugu     1680

auaaugaacu uaccaagguu cgcuacauca acgaucaggg caaaacaagc uauuucucug     1740

gccaggagaa ggagcagauu uucaaugacc uguuuaagca aaaacggaag gugaaaaaga     1800

aagaucucga acuguuccug agaaacauga gccaugucga guccccgacc aucgaagggc     1860

uugaggacuc uuuuaauagc uccuacagca cauaucacga ucuccugaag gugggaauua     1920

agcaggaaau ccuggacaac ccugucaaua ccgagaugcu cgagaacauu gugaaaaucc     1980

ugacgguuuu cgaagauaag cgcaugauca aagagcagcu ucagcaauuu ucugacgugc     2040

uggauggcgu cgugcucaag aaacuggaac ggagacauua cacagggugg ggacgccugu     2100

ccgcuaagcu ccucaugggc auucgggaca agcagagcca ccugaccauc cuggauuauc     2160

ucaugaauga cgacgggcug aacagaaacc uuaugcagcu gauuaaugau ucuaaccucu     2220

ccuucaaaag caucauugag aaggagcagg uuaccacagc cgacaaagau auccaaucca     2280

ucguggcuga ccuggccggc ucucccgcga uuaagaaagg aauccugcag agccuuaaga     2340

uugucgauga acucgugucc gugaugggcu acccucccca gaccaucguu gucgagaugg     2400

cccgggaaaa ucagacaacg gggaaaggaa agaacaauuc ucgccccaga uacaagagcc     2460

uggagaaagc uauuaaggaa uuuggcuccc aaauccugaa agagcauccu accgacaacc     2520

aggagcuccg gaauaacaga cuguaucuuu acuaucugca gaaugggaag gauauguaca     2580

caggacaaga ccucgauauc cacaaccuga gcaauuauga cauugaucac aucgugccgc     2640

agucuuucau uaccgacaac uccaucgaua aucuggugcu uacaagcucu gccggcaacc     2700

gcgaaaaagg ggacgacgug cccccucucg agauugugcg gaagagaaag guguuuuggg     2760

aaaaacugua ccagggcaau cugaugucca agcgcaaauu cgauuaucuc accaaggcug     2820

agagaggagg ccugacggaa gccgacaaag cccgguuuau ccaucgccag cuuguugaga     2880

ccagacaaau uacaaagaac gucgcuaaca uccugcacca gcgguucaau uacgagaagg     2940

augaccaugg gaacaccaug aaacaggugc gcaucguuac acucaagagc gcgcuggugu     3000

cucaguuuag aaaacaauuc cagcuguaca agguccggga ugugaaugac uaucaccacg     3060

cccaugaugc uuaccuuaac ggaguugugg ccaauacccu gcucaaaguc uauccccagc     3120

uggaaccuga guucguguac ggcgacuauc accaguuuga uugguucaag gccaacaagg     3180

cuacggccaa aaagcaauuu uacacaaaua uuaugcuuuu cuuugcgcag aaagaccgca     3240

ucauugauga aaacggggag auccuguggg acaagaaaua ucuugacacc gugaagaagg     3300

ucaugagcua cagacagaug aauauuguua aaaagacaga aauccaaaaa ggagaguucu     3360

ccaaggcuac caucaaaccc aagggcaaca gcucuaaacu gauuccgcgg aagaccaauu     3420

gggauccuau gaaguacggg ggccucgacu cccccaacau ggccuaugcc guggugaucg     3480

aguacgcuaa aggaaagaau aaacuggucu uugaaaagaa aauuauccgc gugacaauua     3540

uggagagaaa ggccuucgaa aaggaugaga aagcguuucu ggaggaacag ggcuaucggc     3600

agccuaaggu ucuugcuaaa cugcccaagu acacgcucua ugagugugaa gaggggagac     3660

gccggaugcu ggccagcgcc aacgaagcuc agaaaggaaa ucaacaggug cucccuaacc     3720

aucuggucac ccuucugcac caugccgcua acugcgaggu guccgacggc aagucucucg     3780

auuacaucga gagcaauaga gaaauguucg ccgagcugcu ggcgcacguu uccgaauuug     3840

ccaagcgcua uacacuugcu gaggccaacc ugaauaaaau caaccagcuc uuugaacaga     3900

auaaggaggg ggacauuaaa gcuaucgccc aaucuuucgu ggaucugaug gccuuuaacg     3960

cuaugggagc gcccgccagc uucaaguuuu ucgagaccac aauugaacgg aaaagauaca     4020

auaaccucaa ggagcugcuu aauuccacca ucauuuacca gucuaucacg ggccuguaug     4080

aaagccgcaa gcggcuggac gauaaaagac cggcugccac aaagaaagcc gggcaggcua     4140

agaaaaagaa aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     4200

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     4260

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     4320

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     4440

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           4480


<210>  4754
<211>  4513
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4754
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaagaaa ccguacagca      120

uuggacugga caucggcacc aacuccguug ggugggcggu ggugacagau gacuauaagg      180

uccccgcuaa gaaaaugaag gugcucggaa auacggauaa aucucauauu gagaagaacc      240

ugcuuggcgc ccuccuguuc gacagcggga auaccgccga agaucggcgc cugaaaagaa      300

cagcucggcg cagauacacc cggcgcagaa accggauccu uuaucugcag gagauuuucu      360

ccgaagagau gggaaagguu gacgauucuu ucuuucaccg ccucgaagac agcuuccugg      420

ugacagagga uaagagaggc gagcggcauc cuaucuuugg caaucuggaa gaggaaguca      480

aauaccacga gaacuucccc accaucuauc aucuccgcca auaccuggcc gacaauccug      540

agaaggugga ucuuagacug guuuaucucg cucuggccca cauuaucaaa uuucgggggc      600

acuuccuuau ugaaggaaag uuugacacac gcaacaauga ugugcagaga cuguuccagg      660

aguuucucgc ggucuacgac aacaccuucg aaaauuccuc ucugcaggag caaaacgucc      720

agguggaaga gauccugacg gacaaaauua gcaaguccgc caaaaaggau cgggugcuca      780

agcuguuucc caaugagaaa agcaacggca gauucgcuga auuucuuaag cugaucguug      840

ggaaucaggc cgacuuuaaa aagcauuucg agcucgaaga gaaagcuccu cugcaguuuu      900

cuaaggauac cuacgaagag gagcuugaag ugcugcucgc ccaaauugga gacaacuaug      960

cggagcuguu ccuguccgcc aagaaacucu acgauagcau ccugcuuucu ggcauccuga     1020

cagugaccga cgucgggaca aaggcuccgc ucuccgccag caugauucag cgcuauaacg     1080

aacaccagau ggaucuggcu cagcuuaaac aauuuauccg gcagaagcug ucugacaaau     1140

acaaugaggu guucuccgau guuagcaagg acggauaugc cggcuacauu gauggcaaga     1200

ccaaccagga agccuuuuau aaauaccuca aggggcugcu gaauaaaauc gagggauccg     1260

gcuacuuccu cgacaagauu gagagagaag acuuucugcg caaacaacgg acguucgaua     1320

acgggucuau cccccaucag auccaccuuc aggagaugag agcuauuauc cgccggcagg     1380

cggaauuuua uccuuuccug gccgacaauc aagauagaau ugagaagcuc cugacauuuc     1440

gcauccccua cuauguggga ccucuggcuc ggggcaagag cgacuucgcc uggcuuucca     1500

gaaaaagcgc cgauaagauu acccccugga acuucgacga gaucgucgau aaagaaucuu     1560

ccgcugaggc cuuuaucaau cgcaugacaa acuacgaccu cuaucugccg aaucagaagg     1620

ugcugccuaa acacagccuc cuguacgaaa aguucaccgu uuauaacgag cuuaccaaag     1680

ugaaguacaa gacagaacag gggaaaacgg cguuuuucga ugcuaauaug aagcaggaga     1740

uuuuugacgg agucuucaaa guguaccgga aggucaccaa agauaagcug auggacuuuc     1800

ucgagaagga guucgaugaa uuuagaaucg uggaccugac aggccuggac aaagaaaaca     1860

agguuuucaa ugccucuuau ggcaccuacc augaucuuug caaaauucuc gacaaggauu     1920

uucuggacaa cagcaaaaau gagaagaucc uggaagauau ugugcucaca cugacccuuu     1980

ucgaggaccg ggagaugauc cgcaagagac uggaaaacua uuccgaucuc cugacgaaag     2040

agcaagucaa gaaacuggaa cggagacacu acacagggug gggacgccuu ucugccgagc     2100

ucauucaugg cauccggaac aaggaaagca gaaaaaccau ccuggacuau cugauugaug     2160

acggaaauuc caaccgcaau uuuaugcagc ucaucaacga ugacgcucug agcuucaagg     2220

aggagauugc caaggcucag gugaucgggg aaaccgauaa ucuuaaccag guugugucug     2280

acauugccgg cucccccgcg aucaaaaagg ggauccugca aagccucaaa auugucgacg     2340

agcuggugaa gaucaugggc caccagccug aaaauauugu gguugagaug gccagagaga     2400

accaguucac aaaucaggga cggcgcaacu cucaacagag acugaaaggc cuuaccgauu     2460

ccaucaagga auuugggagc cagauucuca aagagcaucc cgucgaaaau ucccaacugc     2520

agaacgaccg gcuguuccuc uacuaucugc agaauggacg cgauauguac acaggcgagg     2580

aacuugacau cgauuaccug ucucaguaug acaucgauca cauuaucccc caggcuuuua     2640

uuaaggacaa cagcaucgau aauagagugc ucacguccuc uaaggagaac cgggggaaaa     2700

gcgacgaugu gccuuccaag gacgucgugc gcaaaaugaa aagcuacugg ucuaaacugc     2760

uguccgccaa acuuauuacc caaagaaagu ucgacaaccu gacaaaagcu gagcggggag     2820

gccucaccga ugacgauaag gccggguuua ucaaacgcca gcugguugaa acaagacaga     2880

ucaccaagca cguggcgcgg auucucgacg agagauucaa uacggaaacc gaugagaaca     2940

auaaaaagau ccgccaaguc aagauuguga cacugaaaag caaccuuguu ucuaauuuuc     3000

ggaaggaguu cgaacuguau aaagugagag agaucaacga cuaccaucac gcccaugaug     3060

cuuaucucaa ugccgucauu ggcaaggcuc ugcugggagu guacccgcag cuugaacccg     3120

aguuuguuua uggcgacuac ccucacuucc acgggcauaa agaaaacaag gccaccgcca     3180

agaaauuuuu cuacuccaau aucaugaacu ucuuuaagaa agaugacgug cgcacagaua     3240

agaauggaga gauuaucugg aaaaaggacg aacacaucag caacauuaag aaaguccugu     3300

cuuaucccca ggugaauauc gugaagaaag ucgaggagca gaccggcggg uucagcaagg     3360

aauccauucu cccuaaagga aacagcgaua agcugauccc ccggaaaacg aagaaguuuu     3420

acugggacac aaagaaguau ggcggguucg acucuccgau uguugcuuac uccauccucg     3480

ugaucgcgga uauugagaaa ggcaagagca aaaagcugaa gaccgugaaa gcccuugucg     3540

gagugacaau cauggaaaag augaccuuug agagagaccc uguugcuuuc cuggagcgca     3600

aaggcuaucg gaaugugcaa gaagagaaca uuaucaagcu ccccaaauac ucccuguuua     3660

agcuggaaaa cgggagaaag cgccuucugg ccucugcccg ggagcuccag aaaggaaaug     3720

aaauuguccu gccuaaccau cucggcaccc ugcuuuauca cgcuaagaau auccauaaag     3780

uggaugagcc caagcaccug gacuacguug auaaacacaa ggacgaguuc aaggaacugc     3840

ucgauguggu cagcaacuuu uccaaaaagu acacacuggc cgaggggaau cuugaaaaga     3900

ucaaggagcu guaugcgcag aacaauggag aagaccucaa agagcuggcu ucuagcuuca     3960

uuaaccuccu gacguuuacc gccaucggcg ccccugcuac auucaaguuu uucgauaaaa     4020

auauugacag aaagcgguac accuccacaa ccgagauccu uaacgccacg cugauucauc     4080

aaucuaucac agggcuguau gaaacccgca uugaucucaa uaagcugggc ggagacaaaa     4140

gacccgcggc uaccaagaaa gccggccagg ccaagaaaaa gaagggacua gugcaucaca     4200

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     4260

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     4320

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     4380

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     4500

agccaccaga auu                                                        4513


<210>  4755
<211>  3646
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4755
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcccagaca acaaaccuga      120

gcuacauucu cggacuggac cuuggcaucg cguccguugg gugggcugug guggagauua      180

augaaaacga ggauccgauc ggacugauug acgucggcgu gcggaucuuc gaacgcgccg      240

agguucccaa gacgggggaa ucucucgccc ugagcagacg gcuugcucgc uccaccagac      300

ggcugauccg cagacgggcc caucgccucc ugcuggcuaa aagauuucuc aagcgggagg      360

ggauucuguc uacaaucgau cuugagaagg gccugccuaa ucaagccugg gaacuccgcg      420

uggcgggccu ggagagacgg cuuagcgcca uugaaugggg agcuguccug cuccaccuga      480

ucaaacaucg cggauaucug uccaagcgga aaaacgaguc ucagaccaau aacaaggagc      540

ucggcgcccu gcuuagcggg guggcucaga aucaccagcu gcuccaaucc gacgauuaca      600

gaacacccgc cgaacuggcg cuuaaaaagu ucgccaagga ggaaggacau auuagaaacc      660

agcgcggcgc uuauacccac acauuuaauc ggcuggaccu ccuggccgag cugaaccucc      720

uguucgcuca gcagcaccaa uuugggaauc cucauugcaa agaacacauc cagcaguaca      780

ugaccgagcu ucugaugugg cagaagcccg cccucagcgg agaggccauu cugaaaaugc      840

uuggcaagug uacgcaugaa aagaacgagu ucaaagcugc gaaacacacc uauucugccg      900

aaagauuugu uuggcugaca aagcucaaua accugcgcau ccuggaggau ggcgcugaac      960

gggcccucaa ugaggaggaa agacaacugc uuaucaacca cccuuacgag aaguccaaac     1020

ugaccuaugc ccaggugcgc aagcuccugg ggcugagcga acaggcuauu uucaaacauc     1080

uucgguacuc uaaggagaac gccgaauccg cgacauuuau ggagcucaag gcuuggcacg     1140

ccaucagaaa agcccuggag aaucaaggac ugaaggacac cuggcaggau cucgcuaaaa     1200

agccggaccu gcuugaugaa auuggcacgg ccuucagccu guacaaaaca gacgaggaua     1260

uccagcagua ucucaccaac aaggucccca auucugugau uaacgcucug cugguguccc     1320

uuaauuuuga caaauucauc gaacucagcc ugaagucccu gcgcaagauc cucccucuga     1380

uggagcaagg gaaacgguac gaccaggccu gcagagagau uuauggacau cacuacggcg     1440

aagcgaacca gaagacaucu cagcuucugc ccgccauccc ugcucaagag auucgcaauc     1500

ccgucguucu ccggacccug agccaggcca gaaaagugau caacgcuauu auccggcagu     1560

augggucccc ggcccgcgug cauaucgaaa ccggaagaga gcugggcaag agcuuuaaag     1620

aacggagaga gauucagaag caacaggagg auaaucgcac aaagcgggaa ucugcggucc     1680

agaaauucaa ggagcuuuuc uccgacuuua gcucugaacc uaaaagcaag gauauccuca     1740

aauucagacu guacgagcag caacacggca agugucugua uuccgggaaa gaaauuaaca     1800

uccaccgccu caaugagaag ggauacgugg agauugacca ugcccugccc uuuucuagaa     1860

cgugggauga cagcuucaac aauaagguuc uugugcuggc uuccgaaaac cagaauaaag     1920

gcaaccagac cccuuacgag uggcuccaag ggaagaucaa cagcgaacgg uggaaaaauu     1980

uugucgcccu ggugcuggga ucucagugcu ccgcugccaa gaaacagcgc cuucucacac     2040

agguuauuga ugacaacaag uucaucgaua gaaaucugaa cgacacccgg uauaucgccc     2100

gcuuucugag caauuacauu caagagaacc uccugcuugu gggcaagaau aaaaagaacg     2160

ucuucacacc caaugggcag aucaccgcuc ugcucagauc ucgguggggc cugauuaaag     2220

cgcgcgaaaa caauaacaga caccaugccc uggaugcuau cguggucgcc ugugccacgc     2280

ccuccaugca gcagaagauu acacgguuua uccgcuucaa agaggugcac ccuuauaaga     2340

ucgagaauag auacgaaaug guugaccaag agagcggaga aauuaucucc ccgcacuuuc     2400

ccgagccuug ggcuuauuuc cggcaggagg ugaacauuag agucuuugac aaucaucccg     2460

auaccgugcu uaaggaaaug cugccugacc gcccccaggc caaccaccag uucguucaac     2520

cucucuucgu gucucgggcg ccgaccagaa aaaugagcgg ccaggggcau auggagacaa     2580

ucaaguccgc uaaacgccug gccgaaggaa uuucuguccu ccggaucccc cugacccagc     2640

uuaagccuaa ccugcucgag aauaugguga acaaagaaag agagcccgcc cuguacgcug     2700

gccugaaggc ccgccuugcg gaguuuaauc aagauccugc uaaggccuuc gccacacccu     2760

uuuauaaaca agggggacaa caggugaagg cuauccgggu ugaacagguc cagaaaagcg     2820

gcgugcuggu gagagagaac aauggggucg ccgacaacgc uuccauugug cgcacggaug     2880

uuuucaucaa gaauaacaaa uuuuuccucg ugccgauuua caccuggcag gucgccaagg     2940

gcauccugcc uaauaaagcg auuguggccc acaagaacga agacgagugg gaagagaugg     3000

augagggagc uaaguuuaaa uucagccucu uucccaauga ccugguugaa cuuaagacca     3060

aaaaggagua cuucuuuggc uauuacaucg ggcuggaucg ggccacagga aacaucucuc     3120

ucaaagaaca ugacggcgag auuuccaagg ggaaggaugg aguguauaga gucggcguga     3180

aacuggcucu gagcuucgaa aaguaccagg uugacgagcu ugggaaaaau cggcagaucu     3240

gccgccccca gcagagacag ccugugcgga agcgcccggc cgccaccaaa aaggcuggcc     3300

aagcgaagaa aaagaaagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     3360

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     3420

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     3480

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     3600

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    3646


<210>  4756
<211>  3724
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4756
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccgcggcu uucaagccga      120

accccauuaa uuacauccug ggacucgaca uuggcaucgc cagcguuggg ugggccaugg      180

uggagauuga ugaagacgag aacccuaucu gccugaucga ucuuggagug cgggucuuug      240

aacgcgcuga ggugcccaaa accggcgacu cccuggccau ggcuagacgg cucgcccgcu      300

cuguuagacg gcugacacgc agacgggcgc aucgccuucu gagagcccgg cgccuccuga      360

agagagaagg ggugcugcag gcugccgauu ucgacgagaa uggacucauu aagagccugc      420

cuaacacgcc cuggcaacuu cgggcugccg cgcuggaucg caaacucacc ccucuggagu      480

gguccgccgu ccuucugcac cucaucaagc auagaggcua ucugucucag cggaaaaaug      540

aaggcgagac agcugacaag gaacuggggg cccuccugaa aggaguggcu gauaacgccc      600

acgcccuuca gaccggcgac uuuagaacac cggcugagcu ggcgcucaau aaguucgaga      660

aggaaagcgg gcauauucgc aaccagcggg gagauuacuc ccacaccuuu ucuagaaaag      720

accugcaagc cgagcuuauc cuccuguucg aaaagcagaa agaguuuggc aauccccacg      780

uuagcggggg acugaaggaa ggcauugaga cacugcucau gacccagcgc ccugcucugu      840

ccggcgaugc cgugcagaaa augcuugggc auuguacguu cgagcccgcc gaaccuaagg      900

cugccaaaaa caccuauaca gcggagcggu uuaucuggcu gaccaagcuc aauaaccugc      960

gcauucuuga acaaggaagc gagagacccc ugacagacac cgaacgggcu acgcucaugg     1020

acgagccgua cagaaagucu aaacugacau augcccaggc ccgcaagcug cucggccugg     1080

aggauaccgc uuucuuuaaa gggcuucggu acggaaagga caaugccgag gcuuccacac     1140

ugauggaaau gaaagccuau cacgcgauca gcagagcccu cgaaaaggag ggccugaagg     1200

auaaaaaguc uccucugaac cuuucccccg aacuccagga cgagaucggg accgcuuuca     1260

gccuguuuaa aaccgaugag gacauuacag gacgccugaa ggaucggauc cagccugaaa     1320

uucucgaggc ccugcuuaaa cauaucucuu ucgacaaguu cguccaaauu ucccugaagg     1380

cucucagacg gaucgugccc cugauggaac agggcaaacg cuacgaugag gccugcgcgg     1440

agaucuacgg cgaccacuau gggaagaaaa auacggaaga gaagauuuac cugcccccua     1500

ucccggccga ugaaauuaga aaccccgugg uccuucgggc ucucagccag gccagaaaag     1560

uuaucaacgg aguggugcgc cgguauggcu ccccugcucg cauucacauc gagaccgcca     1620

gagaagucgg gaagucuuuu aaggacagaa aagagaucga gaagcggcaa gaagagaauc     1680

gcaaagacag agaaaaggcc gcugcgaaau uccgggagua cuuucccaac uucgugggag     1740

aaccuaagag caaagauauu cugaagcugc gccucuauga gcagcagcau ggcaaguguc     1800

uguacuccgg gaaagagauc aaucuuggca gacugaacga aaagggauau guugagauug     1860

accacgcccu ccccuuuagc cggacauggg augacucuuu caauaacaaa gugcuggucc     1920

ugggcuccga aaaucagaac aaggggaauc aaaccccgua cgaguacuuu aacggaaaag     1980

auaauagccg cgaauggcag gaguucaagg cuagagugga gacaucucgg uuuccucgca     2040

gcaagaaaca gagaauccuu cuccagaagu ucgacgaaga uggcuuuaaa gagcggaacc     2100

ugaaugacac cagauauguu aaccgcuucc ugugccaauu uguggccgau cggaugagac     2160

ucacggggaa gggcaaaaag cgcgucuucg ccuccaaugg acagauuaca aaccugcuuc     2220

ggggguucug gggccugaga aaggugcgcg cugaaaacga ccggcaucac gcccucgaug     2280

cggucguggu ugcuuguucu accguggcca ugcagcagaa aaucaccaga uuuguccgcu     2340

acaaggagau gaaugccuuc gacggaaaaa caauugauaa ggagaccggc gaagugcugc     2400

aucagaaaac acacuuuccc cagccuuggg aguucuuugc ucaggaaguu augauccggg     2460

uguucgggaa gcccgacgga aagccugagu uugaagaggc cgacacgccc gagaaacuga     2520

gaacccuucu cgcggaaaag cugagcuccc ggccggaggc uguccacgaa uaugugacac     2580

cucuguucgu gagccgcgcc cccaacagaa aaaugucugg ccaggggcau auggagaccg     2640

uuaaguccgc caaacggcuc gaugaaggag ucagcgugcu gcgcgugccc cuuacacaac     2700

ugaagcucaa agaccuggag aagaugguca auagagagcg ggaaccuaag cuguacgagg     2760

cucuuaaagc cagacuggaa gcucacaagg augacccggc caaagcguuu gccgagcccu     2820

ucuauaagua cgauaaagcu ggcaaccgca cccaacaggu gaaggccguu cggguggaac     2880

aaguccagaa gacgggggug uggguuagaa aucauaacgg caucgcugac aaugccacca     2940

uggugcgcgu cgauguguuu gagaaaggag acaaguauua ccucguuccu auuuacucuu     3000

ggcaaguggc caaaggcauc cugcccgauc gggcugucgu gcaggggaag gacgaggaag     3060

auuggcagcu cauugacgau uccuucaacu uuaaauucag ccugcacccu aaugaccuug     3120

uggaggucau cacaaagaag gcgagaaugu ucggauauuu ugccuccugc caccgcggca     3180

ccgggaacau uaauauccgg auccaugacc uggaucacaa aauuggaaag aacggcaucc     3240

ucgaagggau uggcguuaaa acagcucugu cuuuccagaa guaccagauc gacgagcugg     3300

gaaaagagau uagacccugu cgccuuaaga agagaccucc cgugcggaaa cgcccggccg     3360

ccaccaagaa agcuggccag gccaagaaaa agaagggacu agugcaucac auuuaaaagc     3420

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     3480

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     3540

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     3720

aauu                                                                  3724


<210>  4757
<211>  3730
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4757
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaaguac cauguuggaa      120

uugacguggg caccuucagc guggggcugg cggcuaucga ggucgaugac gccggaaugc      180

cgauuaaaac acucucccug gugucucaca uccaugauag cggccuugac cccgaugaaa      240

uuaaguccgc cguuacgcgg cuggcuucua gcgggaucgc ccgcagaacc cggcgccucu      300

auagacggaa gcgcagacgg cugcagcaac uugacaaauu uauccagcgc cagggauggc      360

cugugauuga gcuggaagau uacuccgacc cccucuaucc uuggaagguc agagcugagc      420

uggccgcguc uuacaucgcc gaugaaaaag agcggggcga gaagcugagc guggcucucc      480

gccacauugc cagacaucgg ggcuggcgca accccuaugc uaaaguuucc agccuguacc      540

uuccugacgg gccgucugau gccuucaagg cgaucagaga agagauuaag cgggccuccg      600

gacagcccgu gccugaaaca gcuaccgucg gccaaauggu gacacugugc gagcucggga      660

cccugaaacu uagaggagag ggcggggugc ugagcgcccg ccuccagcag ucugacuaug      720

cucgggaaau ccaggagauu uguagaaugc aagaaaucgg acaggagcug uaccgcaaga      780

ucauugaugu cguuuuugcc gccgaauccc ccaaaggcag cgcuucuucc cgggugggca      840

aggacccucu gcagcccggg aaaaauagag cgcucaaggc cagcgacgcu uuccagcgcu      900

accggaucgc cgcccugauu ggaaaccuua gagugcgcgu cgauggcgag aaacggaucc      960

uguccgugga ggaaaagaau cucguuuuug accaccuggu gaaccuuaca ccgaagaaag     1020

agccugaaug ggucaccauu gcugagaucc uggggaucga uagaggacaa cucauuggca     1080

cggccaccau gacagacgau ggggaacgcg cgggagcucg gcccccuacc cacgacacaa     1140

auagaucuau cgugaacagc cggauugcuc cccugguuga uugguggaag accgccuccg     1200

cccuggagca gcaugccaug gugaaagcuc ucagcaaugc cgaggucgac gauuucgacu     1260

cucccgaagg cgcgaaggug caggccuuuu ucgcugaucu ggacgacgau guccacgcca     1320

aacuugacuc ccugcaucuc ccugugggcc gcgcugccua uagcgaggau acgcugguua     1380

gacuuacacg gagaaugcug ucugacgggg uggaucucua caccgcgcgc cugcaagaau     1440

uuggaaucga gccgagcugg acacccccua caccccggau uggcgaaccu gucgggaacc     1500

ccgccgugga cagaguucug aagaccgugu cccgcuggcu cgagucugcu accaagacgu     1560

ggggagcccc ggagagaguc aucaucgaac acgugcggga gggcuucgug accgaaaaac     1620

gcgcuagaga gauggauggg gacaugcggc gcagagccgc ccggaaugcu aagcuguuuc     1680

aggagaugca ggaaaaacuu aacguucagg gcaagccuag ccgcgcggau cuguggagau     1740

aucaauccgu ccagcggcag aauugccagu gugccuacug cggaagcccc auuacauucu     1800

cuaacuccga gauggaccac aucgugccuc gcgcuggcca agggagcacc aauacaagag     1860

aaaaccucgu ggccgucugu caucggugca aucagucuaa aggaaacacc cccuuugcca     1920

uuugggcuaa gaacacgucc aucgagggcg ugagcguuaa ggaagccgug gagagaacac     1980

gccacugggu caccgauacc gggaugcggu ccacagacuu caaaaaguuc accaaagcgg     2040

ugguugagag auuucagcgc gcuacaaugg augaagagau ugacgcccgg ucuauggaaa     2100

gcguggccug gauggcuaau gagcugagau cccgcgucgc ccagcauuuc gcgucucacg     2160

gaacgaccgu gcggguuuau agaggcagcc ugacagcuga agcccgccgg gccuccggga     2220

ucagcggcaa gcugaaauuu uucgacggag ugggcaaguc uagacucgau cggcgccauc     2280

acgcuauuga cgccgcuguc aucgccuuua ccuccgauua cguggcggag acacuggccg     2340

ugagaagcaa ccugaagcaa ucucaggcuc accggcagga ggccccucag uggcgcgaau     2400

uuaccgggaa agacgcugag cauagagccg ccuggcgggu cuggugucaa aagauggaaa     2460

aacucuccgc ucugcuuacg gaggaucuga gagacgaucg cguuguggug augagcaaug     2520

uccggcucag acugggaaac ggcucugcgc acaaggaaac caucgggaaa cugagcaagg     2580

ugaaacuuuc cagccagcuc ucuguuuccg acauugauaa ggccagcucc gaggcucugu     2640

ggugcgcccu gacacgcgag cccggauuug acccgaagga aggccucccu gccaaucccg     2700

agcggcauau cagagugaac gggacccacg ucuaugcugg cgauaauauu ggacuguucc     2760

ccgugucugc cggcagcauc gcgcuucgcg ggggauacgc ugaacugggc uccucuuuuc     2820

accaugcccg gguuuacaaa auuacaagcg ggaagaaacc ugccuucgcu augcucagag     2880

uguauaccau cgaccugcug ccguaccgca accaggaucu uuucuccguc gagcucaagc     2940

cccaaacgau gucuaugaga caggccgaga aaaagcugcg ggacgcgcug gcuacaggaa     3000

augccgaaua ucucggcugg cuggugguug acgaugagcu uguggucgac accagcaaga     3060

ucgccacaga ucaggugaaa gcuguggaag ccgagcuggg gaccauucgc agauggcggg     3120

ucgacggcuu cuuuuccccu agcaagcucc gccugagacc ccugcagaug ucuaaagaag     3180

gaaucaagaa agaguccgcu ccugagcuua gcaagauuau cgaucggccc ggcuggcucc     3240

cugccguuaa caagcuguuc ucugacggga augugaccgu ggucagacgc gauucccugg     3300

gacgggugag acucgaaagc acagcgcacc uucccguuac guggaaagug caaaagcgcc     3360

cggccgcuac caaaaaggcc ggccaggcua aaaagaagaa aggacuagug caucacauuu     3420

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     3480

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     3540

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     3600

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     3720

caccagaauu                                                            3730


<210>  4758
<211>  3430
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4758
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccgcgcgg auucuggcuu      120

ucgacaucgg aauuagcucc aucggcuggg ccuuuucuga gaacgaugaa cucaaggacu      180

gcgggguucg cauuuucacc aaaguggaga auccgaagac aggagaaagc cuggcccuuc      240

ccagacggcu ggcucgcucc gccagaaagc ggcucgcucg cagaaaagcc cggcugaacc      300

aucuuaagca ccugaucgcg aaugaguuua aacucaacua cgaagauuau cagucuuucg      360

acgagagccu ggccaaggcu uacaaaggcu cccugaucuc uccuuaugag cuccgcuuua      420

gagcccugaa ugaacuucug agcaagcaag auuucgcucg ggugauucuc cauaucgcca      480

agcgcagagg guacgacgau auuaaaaacu ccgacgauaa ggagaaagga gcgauccuga      540

aggccauuaa acagaaugaa gagaaacuug cuaacuauca gagcgucggc gaguaccugu      600

auaaagaaua cuuucagaag uucaaggaaa auucuaaaga auuuacgaac gugcggaaua      660

agaaagaguc cuacgaacgc uguaucgccc agagcuuccu caaggacgag cugaaacuga      720

uuuuuaagaa gcaaagagag uucggcuuuu cuuucuccaa aaaguucgaa gaggaaguuc      780

ucagcguggc uuuuuauaaa cgggcccuga aggauuucuc ucaccuuguc gggaacugcu      840

ccuuuuucac cgacgagaaa agagccccca agaauagccc ucuggcuuuu auguucgugg      900

cgcucacacg caucaucaac cugcuuaaua accugaagaa caccgaaggc auucucuaca      960

caaaagauga ccugaaugcc cugcucaacg agguucugaa gaauggaacc cuuacauaua     1020

aacagaccaa gaaacugcuc gggcuguccg acgauuacga guuuaaggga gaaaagggca     1080

cguauuucau cgaguuuaaa aaguacaaag aguucauuaa ggcucuuggg gagcauaacc     1140

ugucucagga cgaucucaau gaaaucgcca aagacauuac ccugaucaag gaugagauca     1200

aacugaagaa ggcccucgcu aaauaugacc ugaaccaaaa ucagauugau agccuuucca     1260

agcuggaguu uaaagaccac cucaacauca gcuucaaggc ccugaaacug gugacacccc     1320

uuaugcucga aggaaagaag uacgaugagg cguguaauga acugaaccug aaagucgcua     1380

uuaaugagga caagaaagau uuucucccug ccuucaacga gaccuacuau aaggacgaag     1440

ugacaaaucc ggugguccug cgggccauca aagaguacag aaagguucuu aacgcucugc     1500

ucaagaaaua uggcaaggug cacaaaauua auaucgaacu ggcccgcgag gugggcaaga     1560

accauucuca gcgggcuaaa aucgaaaagg agcagaacga gaauuacaag gccaaaaagg     1620

acgcggaacu ggagugcgaa aaacuugggc ucaagauuaa cuccaaaaau auccugaagc     1680

ugagacucuu caaagagcaa aaggaauuuu gugccuauag cggagagaag auuaaaaucu     1740

cugaucugca ggacgagaag augcuugaaa uugaucacau cuaccccuau agccgcuccu     1800

ucgacgauuc uuacaugaac aaaguccugg uguuuaccaa gcagaaucaa gagaaacuca     1860

accagacgcc uuucgaggcu uuuggcaaug acagcgccaa guggcagaag auugaaguuc     1920

uggcuaaaaa ccugcccaca aagaaacaga agcggauccu ugauaaaaau uacaaggaca     1980

aggaacaaaa gaacuucaag gauagaaauc ucaacgacac ccgcuauauc gcccggcugg     2040

ugcugaauua cacaaaagau uaucucgacu uucugccucu uuccgaugac gagaacacca     2100

agcugaauga cacccagaaa gggagcaagg uccaugugga ggcgaagucu ggaaugcuca     2160

cauccgcccu gagacacacg uggggcuuca gcgcuaaaga ucgcaacaac caucugcacc     2220

acgccauuga cgcuguuauc auugccuacg ccaauaacuc uaucgugaag gcuuuuuccg     2280

auuucaaaaa ggaacaggag agcaauuccg cggaacuuua ugccaaaaag auuucugagc     2340

ucgacuacaa gaacaaacgg aaguuuuucg agcccuuuag cggguucaga cagaaagucc     2400

uggauaagau cgacgaaauc uucgugucca aaccggagcg gaagaaaccu ucuggagcuc     2460

ugcaugaaga gaccuuucgc aaggaagagg aguucuauca aagcuacggc ggcaaggaag     2520

ggguccucaa agcccuggag cuuggaaaga uuagaaaagu gaauggcaag aucguuaaaa     2580

acggggauau guuucgggug gacauuuuca agcacaagaa aacaaauaag uuuuacgccg     2640

uccccaucua uaccauggau uucgcucuga aagugcuccc uaacaaggcc guugcgagau     2700

ccaaaaaggg agaaauuaag gacuggaucc ugauggauga gaauuacgaa uuuugcuuca     2760

gccuguauaa agacucucuu auccugauuc agacaaagga uaugcaggag cccgaguuug     2820

uguacuauaa cgcuuucacc uccagcacgg ucucucucau cguguccaaa caugacaaua     2880

aguuugaaac acugagcaaa aaccagaaga uucucuucaa gaaugccaac gagaaagaag     2940

ugaucgccaa gucuauuggc auccaaaauc ugaaaguuuu cgagaaguac aucgucagcg     3000

cucuugggga agugaccaaa gccgaguuuc gccagcggga ggacuucaag aaaaagagac     3060

ccgcggcuac caagaaagcc ggccaggcca agaaaaagaa aggacuagug caucacauuu     3120

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     3180

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     3240

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     3300

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3360

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     3420

caccagaauu                                                            3430


<210>  4759
<211>  3841
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4759
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccagcgac cugguucucg      120

gacuggauau uggcaucggg uccgugggag ugggcauucu uaacaagguc accggggaga      180

ucauucauaa aaauucucgg aucuucccgg cggcucaggc cgaaaacaau cuggugcgca      240

gaacaaaccg gcaaggacgc agacucgccc ggcgcaagaa gcacagacgg guucgccuga      300

auagacuuuu ugaggaaagc ggccugauca cggacuucac caaaauuucc aucaaccuca      360

aucccuacca gcugcgggug aagggccuga cagaugagcu cucuaacgag gagcuguuua      420

uugcucuuaa aaauaugguc aagcaucgcg ggaucagcua ucuggacgau gccuccgacg      480

auggaaacuc uagcgugggc gacuacgcuc agauuguuaa agaaaauucc aagcagcucg      540

aaaccaagac accugggcaa auccagcugg agagauauca gaccuacgga cagcuucggg      600

gcgauuucac aguggaaaaa gacgggaaga aacaccgccu gauuaacguc uuucccacca      660

gcgccuauag aucugaggcg cuccggaucc ugcaaacgca gcaggaguuc aauccucaga      720

ucaccgauga auuuauuaac agauaccugg agauccucac aggaaagcgc aaauauuacc      780

auggccccgg caaugaaaag ucccggaccg acuacgggag auaucgcaca agcggagaga      840

cccuggauaa cauuuucggc auccuuauug ggaaaugcac guuuuacccu gacgaguucc      900

gggccgcuaa ggccucuuau acagcucaag aauuuaaccu gcucaaugac cugaacaauc      960

uuaccgugcc gacagagacc aagaaacugu ccaaggaaca gaaaaaccag aucaucaauu     1020

acgugaagaa cgagaaagcc augggacccg cgaaacucuu caaguauauu gccaaacugc     1080

ugagcuguga ugucgcugac aucaagggcu acagaauuga uaaaucuggg aaggccgaaa     1140

uccacaccuu ugaggcuuau cgcaaaauga agacacucga aacgcuggac auugagcaaa     1200

uggaucggga gacccuugac aagcuggccu acguucucac acugaauacc gaaagagagg     1260

gaauccagga agcccuugag cacgaguucg cugauggcuc cuucagccag aaacaggugg     1320

acgaacuggu gcaauuucgc aaggcgaacu ccucuaucuu cggcaaaggg uggcauaauu     1380

uuagcgucaa gcucaugaug gagcugauuc cugaacugua cgagacaucc gaggaacaga     1440

ugaccauccu cacgcggcug ggaaaacaga agacaaccuc uucuuccaac aagaccaaau     1500

auauugauga gaaacuucug acagaagaga ucuacaaucc cgugguugcc aagagcguga     1560

gacaggcuau uaagaucguc aacgccgcca uuaaagaaua uggcgacuuc gauaauaucg     1620

ugaucgagau ggcucgcgaa accaacgagg acgacgagaa gaaagccauu caaaagaucc     1680

agaaggcgaa uaaagaugaa aaggacgcug ccaugcucaa agccgcuaac caguacaacg     1740

ggaaggccga gcugccucac ucuguuuuuc auggacacaa acagcuggcu acaaagauuc     1800

ggcuuuggca ccaacagggc gaaagaugcc ucuauacggg gaagaccauc agcauucaug     1860

aucugaucaa uaacuccaau caguucgagg uggaccacau ccugccccuc ucuauuacau     1920

uugaugacag ccuggccaac aaaguccuug uguacgcgac cgccaaucag gaaaagggcc     1980

aacggacacc guaucaggcu cuggauucca uggacgaugc cuggagcuuc cgcgagcuca     2040

aggcuuuugu cagagagucu aagacccugu ccaacaaaaa gaaggaauac cugcuuacgg     2100

aggaagacau cagcaaauuc gaugugcgga agaaauuuau ugagagaaau cucguugaca     2160

cccgcuacgc cucucgggug guccugaacg cgcugcagga acauuucaga gcccacaaga     2220

ucgauacaaa aguguccguu gugcgcggac aauuuaccag ccagcucaga cggcauuggg     2280

gcauugagaa gacacgcgac accuaucacc accaugcugu cgacgcccug aucaucgcug     2340

ccuccucuca gcuuaaucug uggaagaaac agaagaacac gcucgugagc uacuccgagg     2400

aucaacugcu ggacauugaa acaggggagc uuaucucuga ugacgaauau aaagagagcg     2460

uguucaaggc cccuuaccag cacuucguug auacccucaa auccaaggag uuugaagaca     2520

gcauucuguu cucuuaucag gucgauucca aauuuaauag aaagaucagc gacgcuacaa     2580

uuuacgcgac ccggcaggcc aaggugggaa aagauaaggc ugacgagacc uaugugcugg     2640

gcaaaaucaa ggauaucuac acacaagacg gguacgaugc cuucaugaaa auuuauaaga     2700

aggacaaauc uaaguuucuc auguaccgcc augaccccca gacguucgaa aaagucaucg     2760

agccuauucu ggaaaacuau cccaauaagc agaucaacga gaaaggaaag gaggugcccu     2820

guaauccuuu ucuuaaguac aaagaggaac acggcuauau uagaaaguac uccaaaaagg     2880

ggaacggccc ggaaaucaaa agccugaagu auuacgauuc uaagcucgga aaccacauug     2940

acaucacccc caaagauagc aauaacaagg uugugcugca guccgucagc ccuuggcggg     3000

ccgacgugua cuucaauaaa acaaccggca aguaugagau ccuggggcuu aaauacgcug     3060

aucuccaauu ugaaaaggga acaggcaccu auaagauuuc ucaggagaaa uacaacgaca     3120

ucaagaaaaa ggaggggguu gauuccgaca gcgaguucaa auuuacgcug uauaagaaug     3180

aucugcuccu ggugaaagac acagaaacca aggaacagca acuuuuccgc uuucugucca     3240

gaaccaugcc caagcagaaa cauuacgucg agcucaagcc uuaugauaaa cagaaguucg     3300

aaggaggcga ggcccugauu aaagugcugg ggaacguugc gaauagcggc cagugcaaga     3360

agggacuugg caaaagcaac aucuccauuu acaaggugcg gacagacguc cuggggaauc     3420

aacacaucau caaaaacgag ggagacaagc ccaaacucga uuucaagcgc ccggcugcca     3480

ccaagaaagc cggccaggcu aagaaaaaga aaggacuagu gcaucacauu uaaaagcauc     3540

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     3600

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     3660

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     3780

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     3840

u                                                                     3841


<210>  4760
<211>  4399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4760
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccacccag uucgagggau      120

uuacaaaccu guaccaaguu agcaagacgc uccgguucga acugauuccg cagggcaaaa      180

cccuuaagca uauccaggag cagggguuua uugaagagga caaggcgcgc aaugaucacu      240

auaaagaacu gaagcccauc auugacagaa ucuacaaaac auaugcugau caaugccucc      300

agcuggugca gcuugacugg gagaaccugu ccgccgccau cgauucuuac cggaaggaga      360

aaaccgaaga gacacgcaau gcucucauug aagaacaggc caccuauaga aacgcuaucc      420

augacuacuu cauuggacgg acagauaauc ugaccgacgc caucaacaag cgccacgcgg      480

agauuuauaa gggccuguuu aaagccgaac ucuucaaugg gaaggugcug aaacaacuug      540

gaacggucac cacaaccgag caugaaaacg cucugcucag auccuuugau aaguucacaa      600

ccuacuuuag cggcuucuac gagaaucgga aaaacguguu uucugccgaa gacaucagca      660

cggcuauucc ucaccgcauc guucaggaua auuuccccaa guuuaaagag aacugucaca      720

ucuucacaag acugauuacc gccgugccuu cccuucggga gcauuucgaa aaugucaaga      780

aggcgaucgg cauuuuugug ucuacaagca ucgaggaagu uuucuccuuu cccuucuaua      840

accagcugcu cacccagacc caaauugacc uguacaauca gcugcucggg ggaaucagcc      900

gcgaggccgg cacagagaaa aucaaggggc ugaacgaggu gcuuaaccug gcuauucaga      960

aaaaugauga gacggcccac aucauugcuu cucucccuca uagauuuauc ccgcuguuca     1020

agcaaauucu uuccgaccgg aacacccuga gcuuuauccu cgaagaguuc aaaucugacg     1080

aagaggucau ccaguccuuu ugcaaguaua agacacugcu gcgcaaugaa aacgugcucg     1140

agaccgccga ggcccuguuc aaugaacuua acagcauuga ucugacacac aucuuuauuu     1200

cucacaaaaa gcucgagacc aucuccagcg cucuguguga ccauugggau acgcuuagaa     1260

augcgcugua cgaacggaga auuuccgagc ucacaggaaa aaucaccaag ucugccaaag     1320

agaaggugca gcgcagccug aagcacgaag acauuaaccu gcaggagauc aucuccgcug     1380

ccggcaaaga acucagcgag gccuucaagc agaaaaccuc ugaaauucug ucccaugcuc     1440

acgccgcgcu ugaucaaccc cugccuacaa cccucaagaa acaggaggag aaggaaaucc     1500

uuaagagcca gcuggacucu cuucucgggc uguaucaucu gcucgauugg uuugcugucg     1560

acgagagcaa ugaaguugau cccgaguucu ccgcccggcu gacaggaauu aaacuugaaa     1620

uggagccuuc ucugagcuuc uacaacaagg ccagaaauua ugcuacgaaa aagcccuacu     1680

ccguggagaa auuuaagcuc aacuuccaaa ugccgacccu ggccagcggc ugggacguga     1740

auaaagaaaa gaacaauggc gcuauccugu uugucaagaa cgggcuuuac uaucucggaa     1800

uuaugccuaa acagaagggc cgcuacaaag cccugucuuu cgagcccaca gaaaagaccu     1860

ccgagggguu ugauaaaaug uauuacgacu auuucccuga cgcggccaag augaucccca     1920

agugcagcac acagcugaaa gcugugaccg cccacuuuca gacgcacacc acacccaucc     1980

uccugucuaa caauuucauu gaaccucuug agaucaccaa ggagauuuac gaucugaaca     2040

auccggaaaa agagcccaag aaauuucaaa cagcuuaugc caagaagacc ggagaccaga     2100

aaggcuaccg ggaagcgcuc uguaagugga ucgauuucac gagagacuuu cuguccaaau     2160

acacaaagac cacaagcauu gaucuguccu cucuucgccc uagcucccag uauaaagacc     2220

ucggggagua cuaugccgag cugaaccccc ugcucuacca uaucucuuuc cagcggaucg     2280

cugaaaagga gauuauggau gccguugaaa ccggaaagcu guaucuuuuu caaaucuaca     2340

auaaagacuu cgcuaagggc caccauggca aaccuaaccu gcacacccuc uauuggacag     2400

ggcuguucag ccccgagaau cuggccaaga cguccauuaa acuuaacgga caggccgaac     2460

ucuuuuacag accuaagagc cgcaugaaac ggauggcuca cagacugggc gagaagaugc     2520

ugaauaagaa acucaaggau cagaaaaccc cgauccccga cacacuguac caagagcuuu     2580

augauuacgu gaaccaucgc cugucucacg accucuccga ugaagcgcgg gcccugcugc     2640

cuaaugucau uaccaaggag gugagccaug aaaucaucaa agacagacgg uucacaucug     2700

acaaguuuuu cuuucacguu cccauuaccc uuaacuauca ggcugccaau uccccuagca     2760

aguucaacca gcgcgugaau gccuaccuga aagagcaucc cgaaacgccg aucauuggga     2820

ucgauagagg agagcggaac cucauuuaua ucacagucau ugacucuacc ggcaagaucc     2880

uggagcagag aagccucaac accauccaac aguuugauua ccagaaaaag cuggacaauc     2940

gcgaaaaaga gcggguggcu gccagacagg cgugguccgu cguggggaca auuaaggauc     3000

uuaagcaagg cuaucugagc cagguuaucc acgaaauugu ggaccucaug auccacuacc     3060

aggcugucgu gguucuggag aaccugaauu ucggauuuaa aucuaagcgc accggcauug     3120

ccgaaaaagc cguguaccag caauucgaga agaugcuuau cgauaaacug aacugccucg     3180

uccugaagga cuauccugcu gagaagguug ggggagugcu caaucccuac cagcugacag     3240

aucaguuuac guccuucgcc aaaaugggca cccaaagcgg guuccuguuu uaugugcccg     3300

cgccuuacac auccaagauc gacccgcuga ccggauucgu cgaucccuuu guguggaaaa     3360

caauuaagaa ccaugaaucu agaaaacacu uccucgaggg cuuugacuuc cugcauuaug     3420

augugaagac cggggacuuu auccugcacu ucaagaugaa ucggaaccuu agcuuucagc     3480

gcggccugcc uggauucaug cccgcuuggg acauugucuu ugagaaaaau gaaacgcagu     3540

ucgaugccaa gggcaccccu uuuaucgccg ggaaaagaau ugugcccguu aucgagaacc     3600

aucgguucac aggacgcuac agagaccucu auccugcuaa ugaacugauc gcccuccugg     3660

aggaaaaggg cauuguguuc cgggaugggu ccaacauccu ucccaaacug cuggagaaug     3720

acgauucuca cgcuauugac accauggucg cccucauccg cagcgugcug cagaugagaa     3780

acuccaaugc ggccacagga gaagauuaca uuaacucucc gguucgggac cuuaacggcg     3840

uguguuuuga uagccgcuuc caaaauccug aguggcccau ggacgcugau gccaacgggg     3900

cuuaccacau cgcccugaag ggccagcucc ugcucaauca ucugaaagag uccaaggacc     3960

uuaagcugca gaacggaauu agcaaucagg acuggcuggc cuauauccaa gaacucagaa     4020

acaaacggcc ugcugcgacc aagaaagccg gccaggcuaa gaaaaagaag ggacuagugc     4080

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     4140

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     4200

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     4260

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     4380

uuucagagcc accagaauu                                                  4399


<210>  4761
<211>  4378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4761
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccagcauu uaccaggagu      120

ucguuaacaa guauucccug ucuaaaaccc uccgguuuga acugaucccg caaggaaaga      180

cacuugagaa uauuaaggcg cgcggccuga uccucgacga ugaaaagaga gcuaaggacu      240

acaaaaaggc caaacagauu aucgauaagu aucaucaguu cuuuaucgag gaaauucuga      300

gcuccgugug caucucugag gaccuucugc agaacuacag cgauguguau uucaagcuca      360

aaaaguccga cgaugacaau cugcaaaaag auuuuaaguc ugccaaagac acgauuaaga      420

aacagaucag cgaguacauu aaggauuccg aaaaguucaa aaaccuguuu aaucagaacc      480

ucaucgacgc uaagaaaggg caggagagcg aucugauucu uuggcugaag caaucuaaag      540

acaauggaau cgaacucuuc aaggccaacu ccgacaucac cgauauugac gaggcucugg      600

agaucauuaa gagcuuuaaa ggcuggacaa ccuauuucaa gggguuucac gaaaaucgga      660

aaaacgucua cucuuccaau gauaucccca caagcauuau cuaccgcauc guggacgaua      720

accuuccuaa guuccuggag aauaaagcca aguaugaauc ucucaaggac aaagcgcccg      780

aggccauuaa cuacgaacag aucaagaaag aucuggcuga ggagcugacc uuugacauug      840

auuauaagac auccgaaguu aaucagagag uguucagccu cgacgagguc uucgaaaucg      900

ccaacuuuaa caauuaccug aaccaguccg gaauuaccaa auucaauacg aucaucggcg      960

gcaaguuugu gaacggggag aauaccaagc ggaaaggaau uaacgaauau aucaaucuuu     1020

acucucaaca gauuaacgau aagacacuga aaaaguauaa aaugagcguu cucuucaagc     1080

agauccuguc cgacaccgag agcaaaucuu uugugauuga caaacuugag gaugacuccg     1140

augucgugac aaccaugcag agcuucuacg aacaaaucgc ugccuuuaag acgguggagg     1200

aaaaaucuau uaaggagaca cugagccucc uguucgacga ucugaaagcg cagaagcucg     1260

accuguccaa aaucuacuuu aagaaugaua agucucuuac cgaccugagc cagcaagucu     1320

ucgaugacua uuccguuauc ggcacagccg ugcucgaaua cauuacccag cagaucgcuc     1380

cuaaaaaccu ggauaauccc agcaagaaag agcaggagcu uauugccaag aaaaccgaaa     1440

aggcuaagua ucugucucuc gagacaauca aacuggcccu ggaagaguuu aacaagcauc     1500

gcgacauuga uaaacaaugu agauucgagg aaauccucgc caauuuugcu gcgaucccua     1560

ugauuuucga cgagaucgcc cagaacaagg acaaucuggc ucagauuucc aucaaauacc     1620

agaaccaagg gaagaaggau cuucugcagg ccagcgccga agacgaugug aaagcuauua     1680

aggaccuccu ggaucagacg aacaaucugc uucacaaacu caagaucuuc cauaucucuc     1740

aguccgagga caaagccaac auucuggaua aggacgaaca cuuuuaucug gucuucgagg     1800

agugcuacuu ugaacucgcg aauaucgugc cgcuguauaa caaaauucgg aauuacauca     1860

cccaaaagcc cuacagcgau gagaaguuca aacuuaacuu ugaaaauucc acacuggcua     1920

acggauggga caagaauaaa gagccugaua acaccgccau ucucuucauc aaggacgaua     1980

aauauuaccu gggcguuaug aauaagaaga acaauaaaau cuuugacgac aaggccauua     2040

aagaaaacaa gggggaggga uauaaaaaga ucguguacaa gcugcuuccc ggcgcuaaua     2100

aaaugcuccc uaaggucuuc uuuucugcca aaagcauuaa guucuauaac cccuccgagg     2160

auauccugcg cauuagaaac cacucuacac auaccaaaaa uggcagcccg cagaaggggu     2220

acgaaaaguu ugaguucaac aucgaagacu gucggaaauu uauugauuuc uauaagcagu     2280

ccaucagcaa acacccugag uggaaggacu ucggauuucg cuucucugau acgcaaagau     2340

acaauuccau cgacgaauuu uaccgggagg uggagaacca gggcuauaaa cugacauucg     2400

aaaauauuag cgagucuuac aucgauuccg uugugaacca ggggaagcuc uaucuguuuc     2460

agauuuacaa uaaggacuuc agcgcuuauu cuaaaggacg ccccaaccuu cauacccugu     2520

acuggaaggc ccucuuugau gaaagaaauc ugcaagacgu cguguauaaa cugaacggcg     2580

aggcggagcu uuucuaccgg aagcagagca ucccuaaaaa gauuacccac cccgccaaag     2640

aagcuaucgc caauaagaac aaggauaauc ccaaaaagga guccgucuuu gaauacgacc     2700

ucaucaaaga caagagauuc acagaggaua aauuuuucuu ccacugcccu auuaccauca     2760

acuuuaagag cucuggggcu aauaaguuca acgacgaaau uaaccugcug cucaaagaga     2820

aggccaauga ugugcauauc cuguccauug accgcggaga gcggcaccuu gcguauuaca     2880

cacugguuga uggcaaaggc aacaucauca agcaggacac guuuaauauu aucgggaacg     2940

auagaaugaa aaccaauuau caugacaagc ucgccgcuau ugaaaaggau cgcgacagcg     3000

cccggaaaga uuggaagaaa aucaacaaua uuaaggagau gaaagaagga uaccuguccc     3060

agguggucca cgagaucgcu aagcugguga uugaauauaa cgccaucguu guguucgagg     3120

accuuaauuu uggcuucaag agagggcgcu uuaaagucga gaagcaagug uaccagaaac     3180

ucgaaaagau gcugaucgag aaacugaacu aucucguguu caaggauaau gaauuugaca     3240

aaacaggagg cguucugcgg gccuaccagc uuaccgcucc guucgagaca uuuaagaaga     3300

uggggaaaca gaccggcauu aucuacuaug uccccgcggg auucacgucu aagauuuguc     3360

cugugaccgg cuuugugaac caacuguacc ccaaauauga aagcgucucc aagucucagg     3420

aguucuucag caaauuugac aagaucugcu acaaucucga uaagggguau uucgaguuuu     3480

ccuucgacua caaaaacuuu ggagauaagg ccgcuaaagg caaguggaca auugccucuu     3540

ucgggagcag acugaucaau uuucgcaacu ccgacaaaaa ucauaacugg gauacccggg     3600

aaguguaucc uacaaaggag cuggaaaaac uucugaaaga cuacagcauc gaguacggac     3660

acggcgagug uauuaaggcc gcuaucugcg gggaaucuga uaaaaaguuc uuugccaaac     3720

ucaccuccgu ucugaacacg auucuccaga ugagaaauag caagacaggc accgagcugg     3780

acuaucuuau cucucccgug gcggauguca acggaaauuu cuuugacucc cgccaagcuc     3840

cgaagaacau gccucaggau gccgacgcca auggcgcuua ccacauuggg cugaaaggac     3900

ucaugcugcu gggccggauc aagaacaauc aggaagggaa aaagcugaac cuugugauca     3960

aaaaugagga auauuucgag uuuguucaga acagaaauaa caagcggccc gccgcgacaa     4020

agaaggcugg acaagccaag aaaaagaaag gacuagugca ucacauuuaa aagcaucuca     4080

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     4140

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     4200

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     4320

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       4378


<210>  4762
<211>  4484
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4762
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccga caagaaauac agcauuggac uggauaucgg caccaacucc guuggguggg      180

cggugauuac agacgaguau aaggugccgu cuaagaaauu caagguccuc ggaaauacgg      240

aucggcauag caucaaaaag aaccugauug gcgcucugcu guuugacucc ggggaaaccg      300

ccgaggccac acgccucaaa agaaccgcuc ggcgcagaua cacacggcgc aagaauagaa      360

ucugcuaucu gcaggaaauc uucucuaacg agauggccaa gguggaugac agcuuuuucc      420

accggcuuga agaguccuuu cugguugagg aagauaaaaa gcaugagcgc caccccauuu      480

ucggaaauau cguggacgaa gucgcuuacc augagaaaua uccuaccauu uaccaccuca      540

gaaagaaacu gguggauucu acagacaagg ccgaucugcg gcucaucuau cuggcgcuug      600

cccacaugau uaaauuucgc ggccauuucc ugaucgaggg cgaccucaac cccgauaaua      660

gcgacguuga caagcuguuu auucaacuug ugcagaccua caaccagcug uucgaagaga      720

auccuaucaa cgcuuccggg gucgaugcca aggcuauccu cagcgccaga cugucuaaau      780

cccggcgccu ggaaaaucuc auugcgcagc ugcccggaga gaagaaaaac ggccuuuuug      840

ggaaucugau cgcccucagc cugggacuua cgccuaacuu caagucuaau uuugaccugg      900

cugaagaugc caaacuccaa cuguccaagg acaccuacga ugacgaucug gacaaccucc      960

uggcucagau uggcgaucag uaugccgacc uuuuccuggc cgcuaagaac cucagcgaug     1020

cgauccugcu uucugacauu cugagaguga auacagagau caccaaagcc ccgcucuccg     1080

cuagcaugau caagcgguac gacgagcacc aucaggaucu gacacugcuc aaagcccugg     1140

ugagacaaca gcuucccgaa aaguauaaag agauuuucuu ugaccagucc aagaacgggu     1200

acgccggaua uaucgauggc ggcgcuucuc aggaagaguu cuacaaguuu auuaaaccua     1260

uccuggaaaa gauggacggg accgaggagc uccuggucaa acugaaucgc gaagaucuuc     1320

uccggaagca aagaacguuc gacaacggaa gcauucccca ccagauccac cugggcgagc     1380

ugcaugccau ccuccgccgg caggaagauu uuuauccuuu ccugaaagac aauagagaga     1440

agauugaaaa gauccuuaca uuucgcauuc ccuacuacgu ugggccgcug gcgcggggaa     1500

acuccagauu cgcuuggaug acccgcaaaa gcgaggagac aaucaccccu uggaauuuug     1560

aagagguggu ggauaagggc gccucugccc aauccuucau ugaacggaug accaacuuug     1620

acaaaaaucu ccccaacgag aagguccugc cuaaacacag ccugcuuuau gaguacuuca     1680

caguguauaa ugaacucacg aagguuaaau acgugaccga ggggaugaga aagcccgcuu     1740

uucugucugg agaacagaag aaagccaucg ucgaucuucu cuucaagaca aaccgcaaag     1800

ugaccguuaa gcagcugaaa gaggacuauu ucaagaagau ugaauguuuu gauagcgugg     1860

agaucuccgg cgucgaggac cgguucaaug cuucucuugg cacauaccau gaccugcuca     1920

aaaucauuaa ggauaaagac uuucuggaua acgaagagaa ugaagacauc cuggaggaua     1980

uugugcuuac ccucacgcug uucgaagaca gagagaugau cgaggaacgg cugaagacau     2040

augcccaccu cuuugaugac aaagucauga agcagcugaa gcgcagacgg uacaccgggu     2100

ggggaagacu uagccgcaaa cugauuaacg gcauccggga uaagcaaucc gggaaaacca     2160

uccucgacuu ccuuaagagc gauggauuug cgaacagaaa uuucaugcag cugauucaug     2220

acgauucucu gacauuuaaa gaggacaucc agaaggccca gguguccggc caaggggaca     2280

gccuccacga acacauugcu aaccuggccg gcucucccgc uaucaagaaa ggcauucugc     2340

agaccguuaa gguggucgau gagcucguga aaguuauggg acgccauaag ccugaaaaua     2400

ucgugaucga gauggccaga gagaaccaga caacgcagaa agggcaaaag aacucccggg     2460

aacgcaugaa aagaauugag gaaggaauca aggagcuggg cagccagauu cuuaaggagc     2520

acccggucga aaauacccag cugcagaaug agaaacucua ccuguauuac cugcaaaacg     2580

ggcgggacau guauguggau caggaacuug acaucaaucg ccuguccgau uacgacgugg     2640

aucauauugu uccccagucu uuccucaagg acgauagcau cgacaacaaa guccugacaa     2700

gauccgauaa gaaucgggga aaaucugaca acgugccuag cgaggaagug gucaagaaga     2760

ugaaaaauua uuggcgccaa cuccugaacg cgaaacuuau cacccagaga aaauuugaca     2820

aucugacaaa ggccgagcgg ggcgggcucu ccgagcugga uaaagcuggc uucauuaagc     2880

gccagcuggu ggaaaccaga cagaucacga agcacguugc ccaaauucuu gacagccgga     2940

ugaacaccaa auacgaugag aacgacaagc ugaucagaga agugaaaguc auuacacuca     3000

agucuaaacu gguguccgau uuucgcaagg acuuccaguu cuauaagguu cgggagauca     3060

auaacuacca ccaugcucac gaugccuacc ucaaugccgu ggucggaacc gcucugauua     3120

aaaaguaucc caaacuugaa agcgaguuug uguacggcga cuauaagguu uacgauguga     3180

gaaaaaugau cgcgaagucu gagcaggaaa ucgggaaggc cacagcuaaa uauuucuuuu     3240

acuccaacau uaugaauuuc uuuaagaccg agaucacgcu ggccaacgga gaaauucgca     3300

aacggccucu caucgagaca aauggcgaaa ccggggagau ugucugggac aagggaagag     3360

auuucgccac agugcgcaaa gugcugagca ugccccaggu caacaucguu aagaaaaccg     3420

aggugcaaac cggcggguuu ucuaaggaaa gcauccugcc gaagagaaau uccgacaaac     3480

uuauugcucg gaagaaagau ugggacccua agaaauaugg cggauucgac agccccacag     3540

uggccuacuc uguccuggug guugcgaagg uggagaaggg caaauccaag aaacucaaga     3600

gcgucaaaga acugcucggg aucacgauua uggagcgcuc cucuuuugag aagaacccua     3660

ucgauuuccu ggaagcuaag ggauacaaag aggugaagaa agaccuuauu aucaagcugc     3720

ccaaauauag ccuguuugaa cucgagaaug gccggaagag aaugcuggcc uccgccgggg     3780

aacuucagaa gggaaacgag cuggcucucc cuucuaaaua cguuaauuuc cuguaucucg     3840

ccagccauua cgagaagcug aaaggcuccc ccgaagauaa cgagcagaag cagcuuuucg     3900

uggaacaaca caaacauuau cuggacgaga ucauugaaca gaucucugag uuuagcaagc     3960

gggucauucu ggcggaugcu aaucucgaca aagugcuguc cgccuacaac aagcaccgcg     4020

auaagccgau cagagagcag gccgaaaaca uuauccaccu uuucacccug acaaaucucg     4080

gggcuccugc cgcuuuuaaa uauuucgaca ccacaauuga ucggaagcgc uacaccagca     4140

cgaaagaggu ucuggacgcc acacucaucc aucaaucuau caccggccug uacgaaacca     4200

gaauugaucu uucccagcug ggaggcgaca agcggcccgc ggccacaaag aaggcugggc     4260

aggccaagaa aaagaaauaa ggacuaguaa uaaggaaacu guaugaaugu cugugggcag     4320

gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu     4380

uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4484


<210>  4763
<211>  5267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4763
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa cuucaagauu cugccgaucg cgauugaccu cggaguuaaa aauacaggcg      180

uguuuagcgc uuucuaccag aaggggacau cccuggagcg gcuugauaac aagaauggaa      240

aaguguauga acugucuaag gacagcuaca cgcuccugau gaacaaucgc accgccagac      300

ggcaucaacg cagaggcauc gaucggaaac agcuugucaa gcgccuguuu aaacucauuu      360

ggacagagca gcugaaccug gaaugggaca aggauaccca gcaagccauc uccuuccucu      420

uuaauagacg gggguucucu uuuaucacag acggauauag ccccgaguac cugaacauug      480

ugccugaaca gguuaaggcu auccuuaugg auauuuucga cgauuauaau ggcgaggacg      540

aucuggacuc cuaccucaaa cuggccaccg agcaggaauc uaagaucagc gagauuuaua      600

acaaacuuau gcagaagauc cuggaauuua aacucaugaa gcugugcaca gauauuaaag      660

acgacaaggu guccaccaag acgcugaaag agaucaccag cuacgaguuc gaacuccugg      720

cugauuaccu ugccaauuau ucugaguccc ugaagacaca aaaauuuagc uacaccgaca      780

agcagggcaa ccucaaagaa cugucuuauu accaccauga uaaguauaau auccaggagu      840

uccuuaagcg ccacgcgaca auuaacgaca gaauccugga uacccuccug acggacgauc      900

uggacauuug gaauuuuaac uucgaaaaau ucgauuuuga caagaacgag gagaaacucc      960

agaaucaaga agauaaggac cauauccagg cccaucugca ccacuucguc uuugcuguga     1020

acaaaauuaa guccgagaug gccagcgggg gacggcaccg cucucaguac uuccaagaaa     1080

ucacaaaugu ucuugacgag aacaaucauc aggaaggcua ucugaagaac uuuugugaga     1140

aucuccacaa caaaaaguac uccaaucuga gcgugaaaaa ccuugucaau cugaucggga     1200

accucuccaa ucuggagcug aagccccuca gaaaauacuu caacgauaag auucacgcua     1260

aggccgacca uugggaugaa cagaaauuua ccgagacaua uugccacugg auccugggag     1320

aauggcgggu gggcgugaag gaccaggaua aaaaggacgg ggcgaaauac ucuuauaagg     1380

aucuuuguaa ugagcugaaa caaaagguca ccaaggccgg acucguugac uuccugcugg     1440

aacuugaucc uugccgcacc auucccccuu accucgacaa caacaauaga aaaccgccca     1500

agugucagag ccugauccug aacccuaaau uucucgauaa ucaguauccc aacuggcagc     1560

aauaccugca ggagcuuaag aaacugcagu ccauucagaa uuaucucgac agcuucgaga     1620

cagaucugaa ggugcugaag ucuuccaaag accaaccuua cuuuguggaa uacaagagcu     1680

cuaaccagca gaucgcuagc ggccagcggg acuauaaaga ucuugacgcc agaauccucc     1740

aauucauuuu ugaucgcguc aaggcuuccg acgagcugcu gcucaaugaa aucuacuucc     1800

aggccaaaaa gcugaagcag aaagccucua gcgagcuuga gaagcuggaa uccagcaaaa     1860

agcucgauga ggugauugcu aacucucaac ugucccagau ccugaaaagc cagcauacga     1920

auggcauuuu cgaacagggg accuuucuuc accucguuug caaguauuac aagcaacggc     1980

agagagcgcg cgacucucgg cuguauauca ugcccgagua cagauaugau aaaaagcugc     2040

auaaauacaa caauacagga cgcuucgacg augacaacca gcuccugacc uacuguaauc     2100

acaagccgcg gcagaaaaga uaucaacuuc ugaacgaucu cgccggcgug cugcaggucu     2160

ccccuaauuu ucugaaggac aaaauuggga gcgaugacga ccuuuucauc uccaaguggc     2220

ucguggaaca cauccgcgga uuuaagaagg cuugcgagga uucucugaaa auucagaaag     2280

acaaccgggg ccugcucaac cauaagauca auauugccag aaacacaaaa gggaagugug     2340

agaaggaaau cuucaaucug auuugcaaaa ucgagggaag cgaagauaag aaaggcaacu     2400

acaagcacgg ccuugccuau gagcuggggg uucuccuguu uggagaaccc aaugaggcuu     2460

ccaaaccuga guucgaccgc aagaucaaga aauuuaacuc uauuuacagc uucgcccaga     2520

uccaacagau ugcguuugcu gaacggaagg gcaaugccaa caccugugcc gugugcuccg     2580

cugauaaugc ccauagaaug cagcaaauca aaauuacgga gcccgucgaa gacaacaagg     2640

auaaaaucau ccugagcgcu aaggcccagc ggcuucccgc gauuccuaca cgcaucgugg     2700

acggggccgu caagaaaaug gcuaccauuc uggccaagaa uaucguggau gacaacuggc     2760

agaauauuaa acagguucuc ucugcuaagc accaacugca caucccgauc auuaccgagu     2820

ccaacgccuu cgaauuugag cccgcgcucg ccgaugugaa aggaaagagc cugaaggaca     2880

gacggaaaaa ggcucuugag agaaucucuc cugaaaauau uuucaaagac aagaacaacc     2940

gcaucaaaga guucgccaag ggcauuuccg cuuauagcgg ggccaaucug acagauggcg     3000

acuuugaugg agccaaagag gaacucgacc auaucauucc ccggucucac aagaaguacg     3060

gcacccugaa cgaugaggcu aaucugaucu gcgucacaag aggggacaac aaaaauaagg     3120

gaaaccgcau cuucugucuu agagaucugg cggacaauua uaaacucaag caguuugaaa     3180

cgaccgauga ccuggagauu gagaaaaaga ucgccgauac aauuugggac gcuaacaaga     3240

aagauuucaa guuuggcaau uaccggagcu ucaucaaccu caccccucag gaacagaaag     3300

ccuuucgcca ugcccuguuc cuugcugacg aaaaucccau uaagcaagcc gugaucagag     3360

cgaucaacaa ucggaaccgc acauuuguua augggaccca gagauacuuc gcugaggugc     3420

uggccaacaa uauuuaucuc cgggccaaaa aggagaaccu gaacacggac aagaucuccu     3480

uugauuacuu cggcauuccg accaucggaa augggcgcgg cauugcugaa aucagacagc     3540

uguaugagaa agucgacagc gauauccagg ccuacgcgaa gggagacaaa ccucaggcuu     3600

cuuauuccca ccuuauugau gccaugcugg ccuucugcau cgcugccgac gaacaucgga     3660

acgauggcag cauugggcuc gagaucgaca agaauuacuc ccuguauccc cucgauaaaa     3720

acacaggaga aguguuuacc aaggacauuu ucucucaaau caagauuaca gauaaugagu     3780

uuagcgacaa aaagcuggug cgcaaaaagg cuaucgaggg cuucaacacc cacagacaga     3840

ugacgcggga ugggaucuac gccgaaaauu accuuccuau ucugauccac aaagagcuga     3900

acgaaguuag aaagggauau acauggaaaa auuccgagga aauuaagauc uuuaagggca     3960

aaaaguacga cauucagcaa cucaacaauc uggucuauug ucuuaaauuc guggacaagc     4020

ccaucucuau cgauauucag aucagcaccc uggaggagcu ccgcaacauu cugacaacca     4080

auaacaucgc ggccaccgcu gaauacuauu acauuaaucu caaaacacag aagcugcaug     4140

aguauuacau cgaaaacuac aacacggccc uuggguauaa gaaauacucc aaggagaugg     4200

aguuucugcg gucucuggcu uauagaagcg aacgcgugaa aaucaagucc auugacgaug     4260

ucaaacaagu gcucgacaag gauagcaauu ucaucauugg caagaucacc cugccuuuua     4320

aaaaggagug gcagcggcuu uacagagaau ggcagaacac aaccauuaaa gacgauuaug     4380

aguuccugaa gucuuucuuc aauguuaaau ccaucacaaa gcuccacaag aaagugcgca     4440

aggacuuuag ccugcccauu ucuaccaacg aaggaaaauu ccucgucaag cggaaaacgu     4500

gggauaauaa cuucaucuac cagauccuga augacuccga uagcagagcc gacggcacaa     4560

agccguuuau uccugccuuc gacaucucua aaaacgagau uguggaggcu aucauugauu     4620

ccuuuaccag caagaauauc uucuggcuuc ccaagaacau cgaacugcaa aaaguugaca     4680

auaagaacau uuuugcgauc gauaccucca aaugguucga gguggaaaca cccagcgacc     4740

ugcgcgauau ugggaucgcc accauucagu auaagaucga caauaacucu cggccuaaag     4800

ucagagugaa gcucgauuac guuaucgacg augacagcaa gauuaauuac uuuaugaacc     4860

auucccugcu uaaaucucgg uauccggaua aggugcugga gauccucaaa cagagcacaa     4920

uuaucgaauu ugaguccucu ggauuuaaca agacgauuaa agagaugcug ggcaugaagc     4980

ucgcugggau cuacaaugaa accagcaaca auaagcgccc cgccgccacc aaaaaggcug     5040

gacaggccaa aaagaaaaag uaaggacuag uaauaaggaa acuguaugaa ugucuguggg     5100

caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug aauaauaaac     5160

uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa aaaaaaaaaa     5220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   5267


<210>  4764
<211>  3539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4764
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gcggaacuac auucugggac ucgacaucgg cauuaccagc guuggguaug      180

gaaucauuga uuacgagaca cgcgacguga ucgaugcggg cgugagacug uucaaagaag      240

cuaaugucga gaacaaugaa gggcggcgcu ccaagagagg agcccggcgc cuuaagagac      300

ggcgcagaca ucggauccag cgcgugaaaa agcugcucuu ugacuauaac cugcuuacgg      360

aucacucuga gcugagcggc auuaauccgu acgaagccag aguuaaaggc cucucccaaa      420

agcugucuga ggaggaguuc agcgcugccc ugcuccaucu ggcuaaacgg cgcggggugc      480

acaacgucaa ugaaguggaa gaggacaccg gaaacgagcu uuccacaaag gaacagaucu      540

cuagaaauag caaggcccug gaggaaaaau auguugcgga gcuccagcug gaacggcuua      600

agaaagaugg cgaggugaga ggguccauua accgcuuuaa gaccagcgac uacgucaaag      660

aggccaagca gcugcucaaa gugcaaaagg cuuaucauca gcuggaucag ucuuucaucg      720

acacauacau ugaucugcuc gaaacccgga gaacauacua ugagggaccc ggcgaagggu      780

ccccuuuugg auggaaggac aucaaagagu gguacgaaau gcugaugggc cacugcaccu      840

auuuccccga ggagcuucgc agcgugaagu acgccuauaa ugcugaucug uacaacgccc      900

ucaaugaccu gaacaaucuu gucauuacgc gggacgaaaa cgagaaacug gaauauuacg      960

agaaguuuca gaucaucgaa aacguuuuca aacaaaagaa gaaaccuacc cucaagcaga     1020

uugcgaaaga gauccuggug aaugaggaag auauuaaggg cuacagagug acaucuaccg     1080

ggaaacccga guuuacaaac cugaaggucu aucacgacau caaggauauu accgcccgca     1140

aagaaaucau cgagaaugcu gagcuccugg accagauugc caagauccuu acgauuuacc     1200

aguccagcga agauauccaa gaggaacuga caaaccucaa uucugagcug acccaggaag     1260

agauugagca gaucuccaac cuuaaaggau auacaggcac ccauaaucug agccucaagg     1320

cuaucaaccu gauucuggac gaacucuggc acaccaauga uaaccaaauc gccauuuuca     1380

aucggcugaa acuugugccu aagaagguug accuguccca gcagaaagag aucccgacaa     1440

cgcucgugga ugacuuuauu cugucucccg ucgugaagcg cagcuucauc caguccauua     1500

aaguuaucaa cgccaucauu aagaaauacg ggcugccuaa ugauaucauu aucgaacuug     1560

cucgcgagaa gaacagcaaa gacgcgcaaa agaugauuaa ugaaaugcag aagcggaaca     1620

gacagaccaa cgagcgcauc gaggaaauca uucggacaac cggaaaagag aaugccaagu     1680

aucucaucga gaaaauuaag cugcaugaca ugcaggaagg caaaugucug uacucucucg     1740

aagcuauccc ccuggaggau cuucugaaca auccuuuuaa cuaugaggug gaccacauua     1800

uccccagauc cgucagcuuc gauaauucuu ucaacaauaa ggugcucguc aagcaagaag     1860

agaacagcaa aaaggggaau cggacaccgu uucaguaccu guccucuagc gacuccaaaa     1920

ucagcuacga aaccuucaag aaacacauuc ugaaccuugc caagggaaag ggccgcaucu     1980

cuaaaacgaa gaaagaguau cuccuggagg aaagagauau uaaucgguuu uccgugcaga     2040

aggacuucau caacagaaau cugguugaua cacgcuacgc cacccggggc cucaugaacc     2100

ugcuuagaag cuauuuucgc gugaauaacc uggacgucaa agugaagucu auuaacgggg     2160

gauucaccuc cuuucucaga cggaagugga aauucaagaa agagcgcaau aagggcuaca     2220

aacaucacgc ugaagaugcc cugaucaucg cgaacgcuga cuuuauuuuc aaggagugga     2280

aaaagcugga uaaggccaaa aagguuaugg aaaaucagau guuugaggag aaacaagccg     2340

aaagcaugcc ugagaucgaa acagagcagg aauauaagga gauuuucauc acaccccauc     2400

agauuaaaca caucaaggac uuuaaggauu acaaauauuc ccauagagug gacaagaaac     2460

cuaaccggga gcuuauuaau gacacccucu acucuacgcg caaggaugac aaagggaaca     2520

cccugaucgu caauaaccug aauggacucu acgauaagga caacgauaag cugaagaaac     2580

uuaucaauaa aagccccgaa aagcugcuca uguaucacca cgacccccag acauaccaaa     2640

aacugaagcu gauuauggag caguauggcg augaaaagaa cccucuuuac aaauauuacg     2700

aggaaaccgg gaauuaucuc acaaaguacu ccaaaaagga caacggcccg gugaucaaaa     2760

agauuaagua cuauggaaau aaacugaacg cucaucugga uaucaccgac gauuacccca     2820

acucuagaaa uaaggugguu aaacucagcc ugaagccuua ucgguucgac gucuaccuug     2880

auaacggcgu guauaaauuc gugacgguca agaaucugga cgugauuaaa aaggagaacu     2940

acuaugaggu uaauuccaag ugcuacgaag aggccaaaaa gcucaaaaag aucagcaacc     3000

aggcugaauu uaucgccucu uucuacaaua acgaccugau uaaaaucaau ggggagcugu     3060

aucgcgugau uggagucaac aaugaucuuc ugaacagaau cgaggugaau augauugaca     3120

ucaccuaccg ggaauaucuc gagaacauga augauaagag acccccuaga aucauuaaga     3180

caaucgcguc caaaacccaa agcauuaaga aauacucuac agacauccug ggcaaccucu     3240

augaaguuaa guccaaaaag cacccccaga uuaucaagaa agggaagcgg ccggccgcua     3300

ccaaaaaggc cggacaggcu aaaaagaaga aauaaggacu aguaauaagg aaacuguaug     3360

aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa uuaugucugc     3420

ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      3539


<210>  4765
<211>  4607
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4765
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcccu guucaacaag ugcauuauca uuagcaucaa ucucgacuuu uccaacaaag      180

agaaguguau gaccaagccg uacucuauug gacuggauau cggcacaaau agcguugggu      240

gggcggugau cacggacaac uauaaagugc ccuccaagaa aaugaagguc cuuggaaaua      300

ccucuaaaaa guacauuaag aaaaaccugc ucggcgugcu gcuuuucgau agcgggauca      360

cagcugaagg acggcgccug aagagaaccg cccggcgcag auauacacgg cgcagaaauc      420

ggauucucua ccugcaggag aucuuuucca ccgaaauggc cacacuggac gaugcuuucu      480

uucaacgccu cgacgauucu uuccugguuc cugacgauaa aagagacagc aaguauccca      540

uuuuuggcaa ccuuguggag gaaaaagucu accaugauga guucccuacc aucuaucauc      600

ugcggaagua ccucgccgac uccacgaaaa aggcugaucu gcgccuugug uaccuggccc      660

ucgcgcacau gauuaaguau agaggccacu uucugaucga gggggaguuc aauagcaaaa      720

acaaugacau ccagaagaac uuucaggacu uccuggauac cuacaaugcc auuuuugaau      780

cugaccucuc ccuggaaaac agcaaacagc uugaggagau cguuaaggau aaaauuucua      840

agcuggaaaa gaaagaccgg auccucaagc uguuccccgg agagaaaaau uccggcauuu      900

ucagcgaauu ucuuaagcug aucgugggga accaagcuga uuuccgcaaa ugcuuuaacc      960

ucgacgagaa ggccucucug cauuucucca aggaaagcua ugaugaggac cuggagacac     1020

uccugggaua caucggcgau gacuauuccg augucuuucu uaaagcuaag aaacuguacg     1080

acgccauucu ccugucuggg uuccuuaccg ugacagacaa ugaaaccgag gcgccucuga     1140

gcuccgccau gaucaagaga uauaacgaac acaaagagga ucucgcucug cugaaggaau     1200

acauucggaa uaucagccuc aagacguaca acgagguguu uaaagacgau acaaagaaug     1260

gauaugccgg cuacauugac ggcaaaacca accaggagga uuucuauguc uaccugaaga     1320

aucuucuggc ugaauuugag ggggccgacu auuuccucga aaagaucgau agagaggacu     1380

uucugcgcaa gcagcggaca uucgauaacg gaucuauccc guaccagauu caucugcaag     1440

aaaugagagc cauccuugac aaacaggcua aguuuuaucc cuuccucgcg aagaauaaag     1500

agcgcauuga gaagauccug accuuccgga uuccuuacua cguuggcccc cuggccagag     1560

ggaacuccga uuuugcuugg agcauccgca aacggaauga aaagauuacc ccuuggaacu     1620

ucgaggacgu gaucgauaaa gaaucuagcg ccgaggccuu uaucaauaga augacauccu     1680

ucgaccucua ucugcccgag gaaaaggugc uuccgaagca cucucugcuc uacgagacgu     1740

uuaacgucua uaaugaacug accaaagugc gcuucauugc ugagagcaug cgggacuacc     1800

aguuucugga uuccaagcag aaaaaggaca ucguuagacu uuauuucaaa gauaagcgca     1860

aggugacaga caaagauauu aucgaauacc uccacgccau uuauggauac gacggcaucg     1920

agcugaaggg gaucgagaaa caauuuaaca gcucucuguc caccuaccau gaucuccuga     1980

acauuaucaa ugacaaggag uuccuugaug acagcucuaa cgaagcgauu aucgaagaga     2040

uuauccacac acugaccauc uuugaagauc gggagaugau uaaacagaga cucuccaagu     2100

ucgagaauau cuuugacaag agcguccuga aaaagcuguc ccggcgccac uauacgggau     2160

ggggcaaacu uucugcuaag cucauuaacg gcaucagaga ugaaaagagc gggaauacaa     2220

uucuggacua ccugaucgac gauggaaucu ccaaccggaa uuucaugcag cucauucaug     2280

acgaugcccu gucuuucaag aaaaagaucc aaaaggccca gauuaucggc gacgaggaua     2340

aagggaacau uaaggaagug guuaaaagcc uuccuggauc ccccgcuauc aagaaaggca     2400

uucugcagag caucaagauc guggacgagc ucgucaaggu gaugggcggg agaaaaccug     2460

aaucuauugu cguggagaug gcccgcgaga aucaguauac caaccaagga aaguccaaua     2520

gccagcagcg gcugaaaaga cuggaaaagu cucuuaaaga gcucggcucc aagauccuga     2580

aggaaaacau ucccgcuaaa cugagcaaga ucgauaauaa cgcccuccag aaugaccgcc     2640

uguaccuuua uuaccugcaa aacgggaaag auauguauac cggagacgau cucgacauug     2700

acagacuguc uaacuacgau aucgaccaca ucauucccca ggcguuucug aaggauaaua     2760

gcaucgacaa caaaguucuu guguccagcg ccucuaaucg gggcaagucc gaugacuucc     2820

cuagccugga ggucgugaag aaacgcaaga cauuuuggua ccagcuccug aaauccaagc     2880

ucauuucuca gagaaaauuc gauaaccuga ccaaggcuga gcgcggggga cuucugccgg     2940

aagacaaggc cggcuuuauc caacggcagc ucguugagac aagacagauu acgaaacaug     3000

uggcucggcu gcuggaugaa aaguucaaua acaaaaagga cgagaauaac cgcgccguca     3060

gaaccgugaa aaucaucaca cuuaagagca cccugguguc ccaguuucgg aaagauuucg     3120

aacucuauaa gguucgcgag auuaaugacu uucaccaugc gcacgaugcc uaccugaacg     3180

cugucaucgc cucugcucuc cugaagaaau aucccaaacu ugagccugag uucguguacg     3240

gggacuaucc caaauacaau agcuuuagag aacggaaguc cgccacagaa aaaguguauu     3300

ucuacucuaa cauuaugaau aucuucaaaa agagcauuuc ccuggccgac ggcagaguca     3360

ucgagcgccc ucucauugaa gugaacgagg agaccggaga aagcguuugg aauaaagagu     3420

cugaucuggc uacggugcgg agaguccugu ccuaccccca agugaacguu gugaagaaag     3480

ucgaggaaca gaaccacggc cuugaccgcg ggaagccgaa aggacuguuu aaugcgaacc     3540

ucagcucuaa gccuaagccc aauuccaacg aaaaucuggu gggcgccaaa gaguaucucg     3600

auccuaagaa auacggggga uaugcuggca ucagcaacuc uuucgccguu cuggugaagg     3660

ggaccaucga gaaaggcgcc aagaagaaaa uuacaaaugu ccuugaauuu cagggaaucu     3720

ccauucugga ccggaucaac uacagaaagg auaaacugaa uuuccuccug gagaagggcu     3780

auaaagacau ugaacuuauc aucgagcugc ccaaguacag ccucuuugag cuguccgaug     3840

ggagccgccg gaugcucgcu ucuauucuga gcaccaacaa uaaaagagga gaaauccaua     3900

agggcaacca aauuuuccuu ucccagaagu uugugaaacu gcuguaucac gccaagcgca     3960

ucucuaauac aauuaacgag aaucaucgga aauacgugga aaaccacaag aaagaguucg     4020

aggaacucuu uuacuauauc cuggaguuca augaaaacua cgucggggcg aagaagaacg     4080

gaaaacuucu gaauagcgcu uuucaguccu ggcagaacca cucuaucgac gagcucugua     4140

gcuccuucau uggcccuacc gggucugaaa gaaagggccu guuugagcuc acgagccggg     4200

gauccgccgc cgauuucgag uuccugggcg uuaaaauccc ccgcuauaga gacuacacac     4260

cgagcucucu ucugaaggau gcuacccuga uucaucaauc cgugacaggg cucuaugaaa     4320

cccggaucga ccuggccaaa cuuggagagg gcaagcgccc ugcggcuacc aagaaagccg     4380

ggcaggccaa gaaaaagaaa uaaggacuag uaauaaggaa acuguaugaa ugucuguggg     4440

caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug aauaauaaac     4500

uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu                   4607


<210>  4766
<211>  3683
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4766
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccug guacgcgagc cugauguccg cucaucaccu ccggguugga auugacgugg      180

gcacccauuc uguggggcug gccacacuuc gcgucgauga ccacggaacg ccgaucgagc      240

ugcucagcgc ccugucccau auucacgauu cuggcguggg gaaggaaggg aaaaaggacc      300

acgauaccag aaagaaacuu agcggcaucg cucggcgcgc cagacggcug cuccaucacc      360

gcagaacaca gcugcaacag cuggacgagg uucuccggga ucugggcuuc cccauuccua      420

cccccgggga auuucuugac cugaacgagc agacagaucc uuaucgcgug uggagagucc      480

gggcucgccu cguggaagag aagcugcccg aggaacuuag aggaccugcc aucuccaugg      540

cgguucggca uaucgcccgc cacagaggcu ggcggaaucc guacucuaaa guggagagcc      600

ugcucucccc cgcugaagag agcccuuuca ugaaggcccu gagagagcgc auucuggcua      660

ccacagggga aguccucgac gauggaauca cccccggcca ggccauggcg caaguggccc      720

ugacgcacaa cauuucuaug cgggggccug agggaauccu uggcaaacug caucaguccg      780

acaaugcuaa cgaaauuaga aagaucugcg cccgccaggg cgugagcccc gaugucugua      840

agcagcuccu gcgggcuguu uuuaaagccg acucuccgag aggguccgcc gugagccgcg      900

uggcuccuga cccccuuccu ggacaaggcu cuuuccggag agcgcccaag ugcgaucccg      960

aguuucagcg cuuccggauu aucuccaucg ucgccaaucu gagaauuagc gaaaccaaag     1020

gggagaaccg cccucucaca gcugacgagc ggagacacgu gguuaccuuu cugacagaag     1080

auucccaggc cgaccugacc uggguggaug ucgccgagaa gcucggagug caucggcgcg     1140

accugagagg cacggcuguu cacacagaug acggggaacg gucugccgcg agaccgccca     1200

ucgaugcuac cgaccgcauu augcggcaga caaaaaucag cucccuuaag accugguggg     1260

aggaagccga uagcgagcaa agaggagcca ugauucgcua ucuguacgag gacccuaccg     1320

acucugaaug ugcugagauc aucgccgaac uccccgagga agaucaggcu aaacuggacu     1380

cccuucaucu gccugccggc agagcggccu auagccggga gucucucaca gcucugagcg     1440

aucacaugcu ggccacgacc gacgaucucc acgaggcucg caagagacug uucggcgugg     1500

acgauuccug ggcgcccccg gccgaagcca uuaaugcucc ugucgggaac cccucugugg     1560

accggacacu uaagaucguc ggacgcuacc ugagcgccgu ggaguccaug uggggcaccc     1620

cugaaguuau ucauguggag cacgucagag auggguuuac aagcgagcgg auggcugacg     1680

aacgcgauaa agccaauaga cggcgcuaua acgacaauca ggaggccaug aagaaaaucc     1740

aaagagauua cggaaaggaa ggcuacauuu cucggggaga caucgugaga cucgacgcuc     1800

uggagcugca gggcugcgcg ugucuuuauu gcggaaccac gaucggcuac cauacauguc     1860

agcucgauca cauuguuccc caggccgggc cuggauccaa caaucgccgg ggcaaccugg     1920

uggcugucug cgaaagaugu aaucgcagca aaucuaacac ccccuucgcc gugugggccc     1980

aaaagugcgg gaucccgcac gugggaguua aggaggcuau uggccggguc agaggguggc     2040

gcaaacagac cccuaacaca uccagcgagg accugacccg gcucaagaaa gaagugaucg     2100

ccagacugcg ccggacacag gaggaucccg aaauugacga gagauccaug gaaucugugg     2160

cguggauggc uaaugagcuu caucaccgga ucgccgccgc uuaucccgag acgaccguca     2220

ugguguaccg cggcagcauu acagccgcgg cuagaaaggc cgccggaauc gauucccgga     2280

ucaaccugau uggcgaaaaa gggcgcaagg acagaaucga ucggagacau cacgcuguug     2340

acgccucugu ggucgcucuc auggaggcca gcguggcgaa gacccuggcc gaacgcucca     2400

gccugcgggg agagcagaga cuuacaggca aagaacaaac cuggaagcag uauacggggu     2460

cuaccguugg agcucgcgag cauuuugaga uguggcgggg ccacaugcuc caccugacag     2520

aacuguucaa ugagagacuc gccgaagaua aaguguacgu cacccagaac auucgccugc     2580

ggcuuuccga cgggaaugcu cauacaguga acccuagcaa gcugguuucu cacagacucg     2640

gcgauggacu gaccgugcag caaaucgacc gcgccuguac gccggcccug uggugcgcuc     2700

uuacaagaga gaaagauuuu gacgagaaga auggccuccc cgcgcgggaa gaccgcgcca     2760

uuagagucca ugggcacgag aucaagucca gcgauuauau ccagguguuc ucuaaacgga     2820

agaaaaccga cagcgaucgc gacgaaacac cuuucggagc uauugccgug agaggcgggu     2880

uugucgagau cggacccucc auucaucacg cccggaucua cagaguugaa ggcaagaaac     2940

cuguguacgc uaugcugcgc guguucaccc acgaucugcu cagccagcgg cauggggacc     3000

uguuuucugc cgucauuccc ccucagucca ucagcaugag augcgcggag cccaaacuuc     3060

gcaaagcuau caccacaggc aacgccacgu aucugggaug ggugguugug ggcgaugagc     3120

ucgaaauuaa ugucgacucc uucaccaagu acgccaucgg gcgguuucug gaggauuucc     3180

cgaacacaac cagauggcgg auuuguggau augacacaaa uucuaagcug acccuuaaac     3240

cuaucgugcu cgcugccgaa ggccuggaga accccagcuc cgcgguuaau gaaauugugg     3300

agcugaaggg guggcgcguc gcuaucaacg ugcucacgaa aguucacccu acagugguca     3360

gacgggaugc ccugggacgc cccagauacu cuagccgguc caaucuuccg accucuugga     3420

ccaucgagaa gcgcccugcc gcuacaaaga aggccggcca agcuaagaaa aagaaauaag     3480

gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag auccuucugu     3540

aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau aaaucuggua     3600

gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaga auu                                             3683


<210>  4767
<211>  4544
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4767
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccac caagccguac agcauuggac uggacaucgg cacaaacucc guuggguggg      180

cggugacgac cgauaauuau aaagugcccu cuaagaagau gaaaguccuc ggaaacacaa      240

gcaagaaaua cauuaagaaa aaucugcuug gcgugcuccu guucgacucc gggaucaccg      300

cugagggacg gcgccugaag agaacagccc ggcgcagaua uacccggcgc agaaaccgga      360

uucuuuaccu gcaggaaauc uuuucuacag agauggccac ccucgaugac gcuuucuuuc      420

aacgccugga ugacagcuuc cugguuccug augacaagag agauuccaaa uaucccaucu      480

uuggcaaucu cguggaagag aaggccuacc augacgaguu cccuacgauu uaucaucugc      540

ggaaauaccu ugcugauucu accaagaaag ccgaccugcg ccucgucuac cuggcgcuug      600

cccacaugau caaguauaga ggccacuuuc ugauugaggg ggaguucaac agcaaaaaua      660

acgauaucca gaagaauuuu caggacuucc ucgacacaua caacgcuauu uuugaauccg      720

aucugagccu ggaaaauucu aagcagcucg aagagaucgu gaaagacaag auuuccaaac      780

uggagaagaa agaucggauc cuuaagcugu uccccggaga aaagaacagc ggcaucuuuu      840

cugaguuccu caaacugauu guugggaauc aagccgacuu ccgcaagugc uuuaaccuug      900

augaaaaggc uucccugcau uucagcaaag agucuuauga cgaagaucuc gagacccugc      960

ugggauacau cggcgacgau uauuccgacg uguuucucaa agccaagaag cuguacgaug     1020

cgauucuucu gagcggguuc cucacaguca ccgacaauga gacggaagcc ccucuguccu     1080

cugcuaugau caaaagauau aacgagcaca aggaagaccu ugcccugcuc aaagaguaca     1140

uucggaacau cagccugaag acauacaaug aaguguuuaa agaugacacc aagaacggau     1200

augcuggcua caucgauggc aagacaaauc aggaggacuu cuauguguac cugaaaaagc     1260

uccuggccga guuugaaggg gccgauuauu uccuugagaa aauugacaga gaagauuuuc     1320

ugcgcaagca gcggaccuuc gacaacggau ccaucccgua ccagauucac cuccaagaga     1380

ugagagcuau ccuggauaaa caggcgaagu uuuaucccuu ccuggccaaa aauaaggaac     1440

gcauugagaa gauccuuacc uuucggaucc cuuacuacgu cggcccccuc gcuagaggga     1500

acagcgacuu cgccuggucu auucgcaaac ggaaugagaa gaucacaccu uggaacuucg     1560

aagauguuau ugacaaagag uccagcgccg aggcuuuuau caauagaaug acgucuuucg     1620

aucuguaucu gcccgaagag aaggugcucc cgaaacauag ccugcuuuac gaaaccuuua     1680

acguguauaa ugagcugaca aagguccgcu ucauugccga auccaugcgg gacuaccagu     1740

uucucgacuc uaagcagaaa aaggauaucg ugagacugua uuucaaagac aagcgcaaag     1800

uuaccgauaa ggacauuauc gaguaccugc acgcgaucua uggauacgau ggcauugaac     1860

uuaaggggau cgagaaacaa uuuaacagcu cccucagcac auaccaugac cugcugaaua     1920

uuaucaacga uaaggaguuc cucgacgauu cuuccaauga agcuauuauc gaggaaauca     1980

uucacacccu gacgaucuuu gaggaccggg aaaugauuaa acagagacuu agcaaguucg     2040

agaacaucuu ugauaaaucu gugcugaaga agcucucccg gcgccacuau acaggauggg     2100

gcaaacugag cgccaagcug auuaacggca ucagagacga gaaauccggg aauaccaucc     2160

uugauuaccu cauugacgac ggaaucucua accggaauuu caugcagcug auucaugaug     2220

acgcccugag cuuuaagaaa aagauccaaa aagcucagau uaucggcgau gaagacaagg     2280

ggaacaucaa ggaggucgug aaaucccucc cuggaucucc cgccauuaag aaaggcaucc     2340

ugcagagcau uaagaucguu gaugaacuug ugaaagucau gggcgggaga aagccugagu     2400

ccauuguggu cgaaauggcu cgcgagaauc aguauaccaa ccaaggaaag agcaauucuc     2460

agcagcggcu gaaaagacuc gagaaguccc ugaaagaacu gggcagcaag auccuuaaag     2520

agaacauucc cgccaagcuc ucuaagaucg acaauaacgc gcugcagaau gaucgccugu     2580

accucuauua ccugcaaaac gggaaagaca uguauacagg agaugaccuu gauaucgaca     2640

gacuguccaa uuacgacauu gaucacauca uuccccaggc cuuccucaag gacaacagca     2700

ucgauaauaa agugcugguu ucuagcgcuu ccaaccgggg caagagcgac gaugugccuu     2760

cucuggaagu cgugaaaaag cgcaagaccu ucugguacca gcuucugaaa uccaagcuca     2820

uuagccagag aaaauuugac aaccugacaa aggccgagcg cgggggacuc uccccggagg     2880

auaaagcugg cuucauccaa cggcagcugg uugaaacgag acagaucacc aagcaugugg     2940

cccggcuucu ggacgagaag uuuaauaaca aaaaggauga aaauaaccgc gcggucagaa     3000

cagugaaaau uaucacccuc aagucuacac uggugagcca guuccggaaa gacuuugagc     3060

uguauaaggu ucgcgaaauu aaugauuucc accaugccca cgacgcuuac cuuaacgccg     3120

ucguggcuuc cgcccugcuc aaaaaguauc ccaagcugga gccugaguuu guguacgggg     3180

auuaucccaa auacaauucu uucagagaaa gaaagagcgc caccgagaaa gucuauuuuu     3240

acuccaacau caugaauauu uucaagaaau cuaucagccu cgcugacggc cgggugaucg     3300

aacgcccucu gauugagguu aacgaagaga cgggagaguc cguguggaau aaggaaagcg     3360

accuugcgac cguccggaga gugcugucuu acccccaagu uaacgugguc aagaaagugg     3420

aggaacagaa ucacggccuc gaucgcggga agccgaaagg acuguuuaac gccaaucugu     3480

ccagcaagcc uaaacccaac ucuaacgaga aucuuguugg cgcuaaggaa uaucuggacc     3540

cuaagaaaua cgggggauau gccggcaucu ccaacagcuu cacagugcuc gucaagggga     3600

ccauugagaa aggcgccaag aaaaagauca caaaugugcu ggaguuccag ggaauuucua     3660

uccucgaucg gauuaacuac agaaaggaca aacugaauuu ucuucuggaa aagggcuaua     3720

aagauaucga gcugaucauu gaacucccca aguacucccu guucgagcuu agcgacgggu     3780

cccgccggau gcuggcuagc auccucucua ccaacaauaa aagaggagag auucauaagg     3840

gcaaccaaau cuuucugagc cagaaauucg ugaagcuccu guaucacgcc aagcgcauuu     3900

ccaauacgau caacgaaaau caucggaaau acgucgagaa ccacaagaaa gaauuugagg     3960

aacuuuucua cuauauccug gaguuuaaug agaacuacgu uggggcgaag aaaaauggaa     4020

agcugcucaa cucugcuuuc cagagcuggc agaaucacuc cauugaugaa cuguguucua     4080

gcuuuaucgg cccuacaggg uccgagagaa agggccuuuu cgaacugacc ucucggggaa     4140

gcgccgccga cuuugaguuc cucggcguga aaauuccccg cuauagagau uacacaccgu     4200

ccagccugcu caaggacgcu acccugaucc aucaaucugu gaccgggcuu uaugaaacac     4260

ggauugaucu ggccaaacug ggagagggca agcgcccugc ggcuacgaaa aaggccgggc     4320

aggccaagaa aaagaaauaa ggacuaguaa uaaggaaacu guaugaaugu cugugggcag     4380

gaagugaaga gauccuucug uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu     4440

uuugaaauaa uaaaucuggu agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4544


<210>  4768
<211>  3626
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4768
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc ggcuuucaag ccgaacagca uuaauuacau ccugggacuc gacauuggca      180

ucgccuccgu ugggugggcc augguggaga uugaugaaga ggaaaacccc auccggcuga      240

ucgaccuugg agugcgcguc uuugagagag cugaagugcc uaaaaccggc gauucucugg      300

ccauggcucg gcgccucgcc agaagcguuc ggcgccugac aagacggcgc gcgcauagac      360

uucugcggac gcgcagacuc cugaagcggg agggggugcu gcaggccgcu aauuucgacg      420

agaacggacu cauuaagucc cugcccaaua ccccuuggca acuucgcgcc gcugcccugg      480

auagaaaacu cacaccccug gaauggucug cgguccuucu gcaccucauc aagcaucggg      540

gcuaucugag ccagcgcaaa aacgagggcg aaaccgccga caaggagcug ggggcucucc      600

ugaaaggagu ggccggcaau gcucacgccc uucagacagg ggauuuuaga accccugccg      660

agcuggcucu caacaaguuc gaaaaggagu ccggacauau ucggaaucag agaucugacu      720

acagccacac auuuucccgc aaagaucugc aagcggaacu uauccuccug uucgagaagc      780

agaaagaauu uggcaacccg cacguuagcg ggggacugaa ggagggcauu gagacccugc      840

ucaugacgca gcggcccgcc cugucuggcg acgcugugca gaaaaugcuu gggcauugca      900

ccuucgaacc ugccgagccc aaggccgcua aaaauacaua uaccgccgaa agauuuaucu      960

ggcugacaaa gcucaacaau cugcgcauuc uugagcaagg auccgaacgg ccucugaccg     1020

auacggagag agcgacacuc auggacgagc ccuaccgcaa gagcaaacug accuaugcuc     1080

aggcccggaa gcugcucggc cuggaagaua cagccuucuu uaaagggcuu agauacggaa     1140

aggacaacgc ugaggccucu acccugaugg aaaugaaggc uuaucacgcc aucucccgcg     1200

cgcucgagaa ggaaggccug aaggacaaaa agagcccgcu gaaucuuucu ccugagcucc     1260

aggaugagau cgggaccgcc uucucccugu uuaaaacaga cgaagauauu acgggacggc     1320

ugaaggacag aauccagccc gagauucucg aagcucugcu uaaacauauc agcuucgaua     1380

aguucgucca aauuucccug aaggcccucc ggcgcaucgu gccucugaug gagcagggca     1440

aaagauacga cgaggcuugu gccgaaaucu acggcgauca cuaugggaag aaaaacaccg     1500

aggaaaagau uuaccugccu cccauccccg cggacgagau ucggaacccg gugguccuua     1560

gagcccucuc ucaggcucgc aaaguuauca auggaguggu gcggagauau ggcagccccg     1620

cccgcauuca caucgaaaca gcuagagagg ucgggaaguc cuuuaaggau cggaaagaga     1680

ucgaaaagcg ccaagaggaa aacagaaaag accgggagaa ggccgccgcu aaauuccgcg     1740

aauacuuucc uaauuucgug ggagagccca agagcaaaga uauucugaag cugagacucu     1800

augagcagca gcauggcaag ugccuguacu cugggaaaga aaucaaccuu ggccggcuga     1860

augagaaggg auauguugaa auugaccacg cgcucccuuu uucccgcacc ugggacgaua     1920

gcuucaacaa uaaagugcug guccugggcu cugagaacca gaauaagggg aaccaaacac     1980

ccuacgaaua cuuuaaugga aaagacaaca gcagagagug gcaggaguuc aaggcccggg     2040

uggaaaccuc ccgcuuuccg agaucuaaga aacagcggau ccuucuccag aaguucgaug     2100

aggacggcuu uaaagaaaga aaucugaacg auacgcgcua uguuaaucgg uuccuguguc     2160

aauuuguggc ugacagaaug cgccucacag ggaagggcaa aaagcggguc uucgccagca     2220

acggacagau uaccaaccug cuuagagggu ucuggggccu gcgcaaggug cgggccgaga     2280

augauagaca ucacgcucuc gacgccgucg ugguugcgug cuccaccgug gcuaugcagc     2340

agaaaaucac acgcuuuguc cgguacaagg agaugaacgc cuucgaugga aaaaccauug     2400

acaaggaaac aggcgaggug cugcaucaga aaacgcacuu uccucagccc ugggaguucu     2460

uugcccagga aguuaugauc agaguguucg ggaagccuga uggaaagccc gaauuugagg     2520

aggcugacac ccuggaaaaa cuucggacac uccuggccga gaagcugagc ucucgcccug     2580

aagcggucca cgaguaugug acaccccucu ucguguccag agcuccgaau cggaaaauga     2640

gcggccaggg gcauauggaa accguuaagu cugccaaacg ccuggaugag ggagucuccg     2700

ugcuuagagu gccucugacc caacucaagc ugaaagaccu ggagaagaug gucaaccggg     2760

aaagagagcc caaacuuuac gaagcccuga aagcucgccu cgaggcccac aaggacgauc     2820

ccgcuaaagc cuuugcggaa ccuuucuaua aguacgacaa agccggcaau cggacgcaac     2880

aggugaaggc uguuagagug gagcaagucc agaagaccgg gguguggguu cgcaaccaua     2940

auggcaucgc cgauaacgcu acaauggugc gggucgacgu guuugagaaa ggagauaagu     3000

auuaccuggu uccgauuuac agcuggcaag uggccaaagg cauccucccc gacagagccg     3060

ucgugcaggg gaaggaugaa gaggacuggc agcugauuga ugacuccuuc aauuuuaaau     3120

ucucucuuca cccuaacgau cugguggaag ucaucaccaa gaaggcucgc auguucggau     3180

auuuugcgag cugucaccgg ggcacaggga auauuaacau cagaauccau gaccucgauc     3240

acaaaauugg aaagaauggc auccuggagg ggauuggcgu uaaaaccgcc cuguccuucc     3300

agaaguacca gaucgacgag cuuggaaaag aaauucgccc cugcagacug aagaagcggc     3360

cucccgugcg caaaagaccu gcugccacga agaaagccgg ccaggcuaag aaaaagaagu     3420

aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa gagauccuuc     3480

uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau aauaaaucug     3540

guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa agaauu                                          3626


<210>  4769
<211>  4382
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4769
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gaaaccguac accauuggac uggacaucgg cacaaacagc guuggguggg      180

cggugcucac ggaucaguau gaccugguga agcggaagau gaaaauugcu ggagauuccg      240

agaagaaaca aaucaagaaa aauuucuggg gcguccgccu uuuugacgaa gggcagaccg      300

ccgccgauag acggauggcu cgcacagcca gacggcgcau ugagagacgg cgcaacagaa      360

ucucuuaccu gcagggaauc uucgcugaag agaugagcaa gaccgacgcc aauuucuucu      420

gccggcucuc cgauucuuuu uauguggaca acgaaaagcg caauagcaga caucccuucu      480

uugcgacaau ugaggaggaa guugaguacc acaaaaacua uccuaccauc uaccaucugc      540

gggaggaacu ugucaauucc ucugagaagg ccgaucugcg ccucgucuau cuggcucugg      600

cccacauuau caaauacaga ggcaacuucc ucauugaagg cgcucuggac acacagaaua      660

ccagcgugga ugggaucuac aagcaauuua uucagacgua uaaccagguu uucgccuccg      720

gaaucgagga cggcagccuu aaaaagcugg aagauaauaa agacguggcg aagauccucg      780

ucgagaaggu gacccggaaa gaaaagcugg agcgcauucu uaaacuguac cccggggaga      840

agucugccgg aauguuugcu caguucaucu cccucauugu gggcagcaaa gggaacuuuc      900

aaaagccuuu cgaccugauc gaaaagucug auauugagug ugccaaagac uccuaugaag      960

aggaucugga aagccuccug gcucuuaucg gagacgagua cgccgagcug uucgucgccg     1020

cuaagaaugc guauucugcc guugugcucu ccagcaucau uacaguggcu gaaaccgaga     1080

caaacgccaa acuguccgcc ucuaugaucg aaagauuuga uacccaugag gaagaccuug     1140

gcgagcugaa ggcuuucauu aaacuccauc ugcccaagca cuacgaggaa aucuuuagca     1200

auacggagaa gcacggcuau gccggguaca uugauggaaa aacaaagcag gcggacuucu     1260

acaaauauau gaagaugacc cuggaaaaca ucgagggcgc ugauuacuuu aucgccaaaa     1320

uugagaagga aaacuuccuc cggaagcaga gaacauuuga caauggggcc aucccucacc     1380

agcugcaucu ugaggaacug gaggcuauuc uccaccaaca ggccaaauau uacccguucc     1440

ugaaggaaaa cuaugauaaa aucaaguccc uugucaccuu ucgcauuccc uacuucgugg     1500

gaccucuggc uaauggccag agcgaguuug ccuggcucac ccggaaagcg gacggggaga     1560

ucagacccug gaacauugaa gagaagguug acuucggaaa aucugccgug gauuuuaucg     1620

aaaagaugac aaauaaggac acguaucugc cuaaagagaa cguccugccc aagcacuccc     1680

ucugcuacca aaaauaccug guguauaaug aacuuaccaa gguucgcuac aucaacgauc     1740

agggcaaaac aagcuauuuc ucuggccagg agaaggagca gauuuucaau gaccuguuua     1800

agcaaaaacg gaaggugaaa aagaaagauc ucgaacuguu ccugagaaac augagccaug     1860

ucgagucccc gaccaucgaa gggcuugagg acucuuuuaa uagcuccuac agcacauauc     1920

acgaucuccu gaagguggga auuaagcagg aaauccugga caacccuguc aauaccgaga     1980

ugcucgagaa cauugugaaa auccugacgg uuuucgaaga uaagcgcaug aucaaagagc     2040

agcuucagca auuuucugac gugcuggaug gcgucgugcu caagaaacug gaacggagac     2100

auuacacagg guggggacgc cuguccgcua agcuccucau gggcauucgg gacaagcaga     2160

gccaccugac cauccuggau uaucucauga augacgacgg gcugaacaga aaccuuaugc     2220

agcugauuaa ugauucuaac cucuccuuca aaagcaucau ugagaaggag cagguuacca     2280

cagccgacaa agauauccaa uccaucgugg cugaccuggc cggcucuccc gcgauuaaga     2340

aaggaauccu gcagagccuu aagauugucg augaacucgu guccgugaug ggcuacccuc     2400

cccagaccau cguugucgag auggcccggg aaaaucagac aacggggaaa ggaaagaaca     2460

auucucgccc cagauacaag agccuggaga aagcuauuaa ggaauuuggc ucccaaaucc     2520

ugaaagagca uccuaccgac aaccaggagc uccggaauaa cagacuguau cuuuacuauc     2580

ugcagaaugg gaaggauaug uacacaggac aagaccucga uauccacaac cugagcaauu     2640

augacauuga ucacaucgug ccgcagucuu ucauuaccga caacuccauc gauaaucugg     2700

ugcuuacaag cucugccggc aaccgcgaaa aaggggacga cgugcccccu cucgagauug     2760

ugcggaagag aaagguguuu ugggaaaaac uguaccaggg caaucugaug uccaagcgca     2820

aauucgauua ucucaccaag gcugagagag gaggccugac ggaagccgac aaagcccggu     2880

uuauccaucg ccagcuuguu gagaccagac aaauuacaaa gaacgucgcu aacauccugc     2940

accagcgguu caauuacgag aaggaugacc augggaacac caugaaacag gugcgcaucg     3000

uuacacucaa gagcgcgcug gugucucagu uuagaaaaca auuccagcug uacaaggucc     3060

gggaugugaa ugacuaucac cacgcccaug augcuuaccu uaacggaguu guggccaaua     3120

cccugcucaa agucuauccc cagcuggaac cugaguucgu guacggcgac uaucaccagu     3180

uugauugguu caaggccaac aaggcuacgg ccaaaaagca auuuuacaca aauauuaugc     3240

uuuucuuugc gcagaaagac cgcaucauug augaaaacgg ggagauccug ugggacaaga     3300

aauaucuuga caccgugaag aaggucauga gcuacagaca gaugaauauu guuaaaaaga     3360

cagaaaucca aaaaggagag uucuccaagg cuaccaucaa acccaagggc aacagcucua     3420

aacugauucc gcggaagacc aauugggauc cuaugaagua cgggggccuc gacuccccca     3480

acauggccua ugccguggug aucgaguacg cuaaaggaaa gaauaaacug gucuuugaaa     3540

agaaaauuau ccgcgugaca auuauggaga gaaaggccuu cgaaaaggau gagaaagcgu     3600

uucuggagga acagggcuau cggcagccua agguucuugc uaaacugccc aaguacacgc     3660

ucuaugagug ugaagagggg agacgccgga ugcuggccag cgccaacgaa gcucagaaag     3720

gaaaucaaca ggugcucccu aaccaucugg ucacccuucu gcaccaugcc gcuaacugcg     3780

agguguccga cggcaagucu cucgauuaca ucgagagcaa uagagaaaug uucgccgagc     3840

ugcuggcgca cguuuccgaa uuugccaagc gcuauacacu ugcugaggcc aaccugaaua     3900

aaaucaacca gcucuuugaa cagaauaagg agggggacau uaaagcuauc gcccaaucuu     3960

ucguggaucu gauggccuuu aacgcuaugg gagcgcccgc cagcuucaag uuuuucgaga     4020

ccacaauuga acggaaaaga uacaauaacc ucaaggagcu gcuuaauucc accaucauuu     4080

accagucuau cacgggccug uaugaaagcc gcaagcggcu ggacgauaaa agaccggcug     4140

ccacaaagaa agccgggcag gcuaagaaaa agaaauaagg acuaguaaua aggaaacugu     4200

augaaugucu gugggcagga agugaagaga uccuucugua aagguuuuug gaauuauguc     4260

ugcugaauaa uaaacuuuuu ugaaauaaua aaucugguag aaaaaugaga ucuaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     4380

uu                                                                    4382


<210>  4770
<211>  4415
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4770
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gaaaccguac agcauuggac uggacaucgg caccaacucc guuggguggg      180

cgguggugac agaugacuau aagguccccg cuaagaaaau gaaggugcuc ggaaauacgg      240

auaaaucuca uauugagaag aaccugcuug gcgcccuccu guucgacagc gggaauaccg      300

ccgaagaucg gcgccugaaa agaacagcuc ggcgcagaua cacccggcgc agaaaccgga      360

uccuuuaucu gcaggagauu uucuccgaag agaugggaaa gguugacgau ucuuucuuuc      420

accgccucga agacagcuuc cuggugacag aggauaagag aggcgagcgg cauccuaucu      480

uuggcaaucu ggaagaggaa gucaaauacc acgagaacuu ccccaccauc uaucaucucc      540

gccaauaccu ggccgacaau ccugagaagg uggaucuuag acugguuuau cucgcucugg      600

cccacauuau caaauuucgg gggcacuucc uuauugaagg aaaguuugac acacgcaaca      660

augaugugca gagacuguuc caggaguuuc ucgcggucua cgacaacacc uucgaaaauu      720

ccucucugca ggagcaaaac guccaggugg aagagauccu gacggacaaa auuagcaagu      780

ccgccaaaaa ggaucgggug cucaagcugu uucccaauga gaaaagcaac ggcagauucg      840

cugaauuucu uaagcugauc guugggaauc aggccgacuu uaaaaagcau uucgagcucg      900

aagagaaagc uccucugcag uuuucuaagg auaccuacga agaggagcuu gaagugcugc      960

ucgcccaaau uggagacaac uaugcggagc uguuccuguc cgccaagaaa cucuacgaua     1020

gcauccugcu uucuggcauc cugacaguga ccgacgucgg gacaaaggcu ccgcucuccg     1080

ccagcaugau ucagcgcuau aacgaacacc agauggaucu ggcucagcuu aaacaauuua     1140

uccggcagaa gcugucugac aaauacaaug agguguucuc cgauguuagc aaggacggau     1200

augccggcua cauugauggc aagaccaacc aggaagccuu uuauaaauac cucaaggggc     1260

ugcugaauaa aaucgaggga uccggcuacu uccucgacaa gauugagaga gaagacuuuc     1320

ugcgcaaaca acggacguuc gauaacgggu cuauccccca ucagauccac cuucaggaga     1380

ugagagcuau uauccgccgg caggcggaau uuuauccuuu ccuggccgac aaucaagaua     1440

gaauugagaa gcuccugaca uuucgcaucc ccuacuaugu gggaccucug gcucggggca     1500

agagcgacuu cgccuggcuu uccagaaaaa gcgccgauaa gauuaccccc uggaacuucg     1560

acgagaucgu cgauaaagaa ucuuccgcug aggccuuuau caaucgcaug acaaacuacg     1620

accucuaucu gccgaaucag aaggugcugc cuaaacacag ccuccuguac gaaaaguuca     1680

ccguuuauaa cgagcuuacc aaagugaagu acaagacaga acaggggaaa acggcguuuu     1740

ucgaugcuaa uaugaagcag gagauuuuug acggagucuu caaaguguac cggaagguca     1800

ccaaagauaa gcugauggac uuucucgaga aggaguucga ugaauuuaga aucguggacc     1860

ugacaggccu ggacaaagaa aacaagguuu ucaaugccuc uuauggcacc uaccaugauc     1920

uuugcaaaau ucucgacaag gauuuucugg acaacagcaa aaaugagaag auccuggaag     1980

auauugugcu cacacugacc cuuuucgagg accgggagau gauccgcaag agacuggaaa     2040

acuauuccga ucuccugacg aaagagcaag ucaagaaacu ggaacggaga cacuacacag     2100

gguggggacg ccuuucugcc gagcucauuc auggcauccg gaacaaggaa agcagaaaaa     2160

ccauccugga cuaucugauu gaugacggaa auuccaaccg caauuuuaug cagcucauca     2220

acgaugacgc ucugagcuuc aaggaggaga uugccaaggc ucaggugauc ggggaaaccg     2280

auaaucuuaa ccagguugug ucugacauug ccggcucccc cgcgaucaaa aaggggaucc     2340

ugcaaagccu caaaauuguc gacgagcugg ugaagaucau gggccaccag ccugaaaaua     2400

uugugguuga gauggccaga gagaaccagu ucacaaauca gggacggcgc aacucucaac     2460

agagacugaa aggccuuacc gauuccauca aggaauuugg gagccagauu cucaaagagc     2520

aucccgucga aaauucccaa cugcagaacg accggcuguu ccucuacuau cugcagaaug     2580

gacgcgauau guacacaggc gaggaacuug acaucgauua ccugucucag uaugacaucg     2640

aucacauuau cccccaggcu uuuauuaagg acaacagcau cgauaauaga gugcucacgu     2700

ccucuaagga gaaccggggg aaaagcgacg augugccuuc caaggacguc gugcgcaaaa     2760

ugaaaagcua cuggucuaaa cugcuguccg ccaaacuuau uacccaaaga aaguucgaca     2820

accugacaaa agcugagcgg ggaggccuca ccgaugacga uaaggccggg uuuaucaaac     2880

gccagcuggu ugaaacaaga cagaucacca agcacguggc gcggauucuc gacgagagau     2940

ucaauacgga aaccgaugag aacaauaaaa agauccgcca agucaagauu gugacacuga     3000

aaagcaaccu uguuucuaau uuucggaagg aguucgaacu guauaaagug agagagauca     3060

acgacuacca ucacgcccau gaugcuuauc ucaaugccgu cauuggcaag gcucugcugg     3120

gaguguaccc gcagcuugaa cccgaguuug uuuauggcga cuacccucac uuccacgggc     3180

auaaagaaaa caaggccacc gccaagaaau uuuucuacuc caauaucaug aacuucuuua     3240

agaaagauga cgugcgcaca gauaagaaug gagagauuau cuggaaaaag gacgaacaca     3300

ucagcaacau uaagaaaguc cugucuuauc cccaggugaa uaucgugaag aaagucgagg     3360

agcagaccgg cggguucagc aaggaaucca uucucccuaa aggaaacagc gauaagcuga     3420

ucccccggaa aacgaagaag uuuuacuggg acacaaagaa guauggcggg uucgacucuc     3480

cgauuguugc uuacuccauc cucgugaucg cggauauuga gaaaggcaag agcaaaaagc     3540

ugaagaccgu gaaagcccuu gucggaguga caaucaugga aaagaugacc uuugagagag     3600

acccuguugc uuuccuggag cgcaaaggcu aucggaaugu gcaagaagag aacauuauca     3660

agcuccccaa auacucccug uuuaagcugg aaaacgggag aaagcgccuu cuggccucug     3720

cccgggagcu ccagaaagga aaugaaauug uccugccuaa ccaucucggc acccugcuuu     3780

aucacgcuaa gaauauccau aaaguggaug agcccaagca ccuggacuac guugauaaac     3840

acaaggacga guucaaggaa cugcucgaug uggucagcaa cuuuuccaaa aaguacacac     3900

uggccgaggg gaaucuugaa aagaucaagg agcuguaugc gcagaacaau ggagaagacc     3960

ucaaagagcu ggcuucuagc uucauuaacc uccugacguu uaccgccauc ggcgccccug     4020

cuacauucaa guuuuucgau aaaaauauug acagaaagcg guacaccucc acaaccgaga     4080

uccuuaacgc cacgcugauu caucaaucua ucacagggcu guaugaaacc cgcauugauc     4140

ucaauaagcu gggcggagac aaaagacccg cggcuaccaa gaaagccggc caggccaaga     4200

aaaagaagua aggacuagua auaaggaaac uguaugaaug ucugugggca ggaagugaag     4260

agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu uuuugaaaua     4320

auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                4415


<210>  4771
<211>  3548
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4771
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccca gacaacaaac cugagcuaca uucucggacu ggaccuuggc aucgcguccg      180

uugggugggc ugugguggag auuaaugaaa acgaggaucc gaucggacug auugacgucg      240

gcgugcggau cuucgaacgc gccgagguuc ccaagacggg ggaaucucuc gcccugagca      300

gacggcuugc ucgcuccacc agacggcuga uccgcagacg ggcccaucgc cuccugcugg      360

cuaaaagauu ucucaagcgg gaggggauuc ugucuacaau cgaucuugag aagggccugc      420

cuaaucaagc cugggaacuc cgcguggcgg gccuggagag acggcuuagc gccauugaau      480

ggggagcugu ccugcuccac cugaucaaac aucgcggaua ucuguccaag cggaaaaacg      540

agucucagac caauaacaag gagcucggcg cccugcuuag cgggguggcu cagaaucacc      600

agcugcucca auccgacgau uacagaacac ccgccgaacu ggcgcuuaaa aaguucgcca      660

aggaggaagg acauauuaga aaccagcgcg gcgcuuauac ccacacauuu aaucggcugg      720

accuccuggc cgagcugaac cuccuguucg cucagcagca ccaauuuggg aauccucauu      780

gcaaagaaca cauccagcag uacaugaccg agcuucugau guggcagaag cccgcccuca      840

gcggagaggc cauucugaaa augcuuggca aguguacgca ugaaaagaac gaguucaaag      900

cugcgaaaca caccuauucu gccgaaagau uuguuuggcu gacaaagcuc aauaaccugc      960

gcauccugga ggauggcgcu gaacgggccc ucaaugagga ggaaagacaa cugcuuauca     1020

accacccuua cgagaagucc aaacugaccu augcccaggu gcgcaagcuc cuggggcuga     1080

gcgaacaggc uauuuucaaa caucuucggu acucuaagga gaacgccgaa uccgcgacau     1140

uuauggagcu caaggcuugg cacgccauca gaaaagcccu ggagaaucaa ggacugaagg     1200

acaccuggca ggaucucgcu aaaaagccgg accugcuuga ugaaauuggc acggccuuca     1260

gccuguacaa aacagacgag gauauccagc aguaucucac caacaagguc cccaauucug     1320

ugauuaacgc ucugcuggug ucccuuaauu uugacaaauu caucgaacuc agccugaagu     1380

cccugcgcaa gauccucccu cugauggagc aagggaaacg guacgaccag gccugcagag     1440

agauuuaugg acaucacuac ggcgaagcga accagaagac aucucagcuu cugcccgcca     1500

ucccugcuca agagauucgc aaucccgucg uucuccggac ccugagccag gccagaaaag     1560

ugaucaacgc uauuauccgg caguaugggu ccccggcccg cgugcauauc gaaaccggaa     1620

gagagcuggg caagagcuuu aaagaacgga gagagauuca gaagcaacag gaggauaauc     1680

gcacaaagcg ggaaucugcg guccagaaau ucaaggagcu uuucuccgac uuuagcucug     1740

aaccuaaaag caaggauauc cucaaauuca gacuguacga gcagcaacac ggcaaguguc     1800

uguauuccgg gaaagaaauu aacauccacc gccucaauga gaagggauac guggagauug     1860

accaugcccu gcccuuuucu agaacguggg augacagcuu caacaauaag guucuugugc     1920

uggcuuccga aaaccagaau aaaggcaacc agaccccuua cgaguggcuc caagggaaga     1980

ucaacagcga acgguggaaa aauuuugucg cccuggugcu gggaucucag ugcuccgcug     2040

ccaagaaaca gcgccuucuc acacagguua uugaugacaa caaguucauc gauagaaauc     2100

ugaacgacac ccgguauauc gcccgcuuuc ugagcaauua cauucaagag aaccuccugc     2160

uugugggcaa gaauaaaaag aacgucuuca cacccaaugg gcagaucacc gcucugcuca     2220

gaucucggug gggccugauu aaagcgcgcg aaaacaauaa cagacaccau gcccuggaug     2280

cuaucguggu cgccugugcc acgcccucca ugcagcagaa gauuacacgg uuuauccgcu     2340

ucaaagaggu gcacccuuau aagaucgaga auagauacga aaugguugac caagagagcg     2400

gagaaauuau cuccccgcac uuucccgagc cuugggcuua uuuccggcag gaggugaaca     2460

uuagagucuu ugacaaucau cccgauaccg ugcuuaagga aaugcugccu gaccgccccc     2520

aggccaacca ccaguucguu caaccucucu ucgugucucg ggcgccgacc agaaaaauga     2580

gcggccaggg gcauauggag acaaucaagu ccgcuaaacg ccuggccgaa ggaauuucug     2640

uccuccggau cccccugacc cagcuuaagc cuaaccugcu cgagaauaug gugaacaaag     2700

aaagagagcc cgcccuguac gcuggccuga aggcccgccu ugcggaguuu aaucaagauc     2760

cugcuaaggc cuucgccaca cccuuuuaua aacaaggggg acaacaggug aaggcuaucc     2820

ggguugaaca gguccagaaa agcggcgugc uggugagaga gaacaauggg gucgccgaca     2880

acgcuuccau ugugcgcacg gauguuuuca ucaagaauaa caaauuuuuc cucgugccga     2940

uuuacaccug gcaggucgcc aagggcaucc ugccuaauaa agcgauugug gcccacaaga     3000

acgaagacga gugggaagag auggaugagg gagcuaaguu uaaauucagc cucuuuccca     3060

augaccuggu ugaacuuaag accaaaaagg aguacuucuu uggcuauuac aucgggcugg     3120

aucgggccac aggaaacauc ucucucaaag aacaugacgg cgagauuucc aaggggaagg     3180

auggagugua uagagucggc gugaaacugg cucugagcuu cgaaaaguac cagguugacg     3240

agcuugggaa aaaucggcag aucugccgcc cccagcagag acagccugug cggaagcgcc     3300

cggccgccac caaaaaggcu ggccaagcga agaaaaagaa auaaggacua guaauaagga     3360

aacuguauga augucugugg gcaggaagug aagagauccu ucuguaaagg uuuuuggaau     3420

uaugucugcu gaauaauaaa cuuuuuugaa auaauaaauc ugguagaaaa augagaucua     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaagaauu                                                              3548


<210>  4772
<211>  3626
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4772
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc ggcuuucaag ccgaacccca uuaauuacau ccugggacuc gacauuggca      180

ucgccagcgu ugggugggcc augguggaga uugaugaaga cgagaacccu aucugccuga      240

ucgaucuugg agugcggguc uuugaacgcg cugaggugcc caaaaccggc gacucccugg      300

ccauggcuag acggcucgcc cgcucuguua gacggcugac acgcagacgg gcgcaucgcc      360

uucugagagc ccggcgccuc cugaagagag aaggggugcu gcaggcugcc gauuucgacg      420

agaauggacu cauuaagagc cugccuaaca cgcccuggca acuucgggcu gccgcgcugg      480

aucgcaaacu caccccucug gagugguccg ccguccuucu gcaccucauc aagcauagag      540

gcuaucuguc ucagcggaaa aaugaaggcg agacagcuga caaggaacug ggggcccucc      600

ugaaaggagu ggcugauaac gcccacgccc uucagaccgg cgacuuuaga acaccggcug      660

agcuggcgcu caauaaguuc gagaaggaaa gcgggcauau ucgcaaccag cggggagauu      720

acucccacac cuuuucuaga aaagaccugc aagccgagcu uauccuccug uucgaaaagc      780

agaaagaguu uggcaauccc cacguuagcg ggggacugaa ggaaggcauu gagacacugc      840

ucaugaccca gcgcccugcu cuguccggcg augccgugca gaaaaugcuu gggcauugua      900

cguucgagcc cgccgaaccu aaggcugcca aaaacaccua uacagcggag cgguuuaucu      960

ggcugaccaa gcucaauaac cugcgcauuc uugaacaagg aagcgagaga ccccugacag     1020

acaccgaacg ggcuacgcuc auggacgagc cguacagaaa gucuaaacug acauaugccc     1080

aggcccgcaa gcugcucggc cuggaggaua ccgcuuucuu uaaagggcuu cgguacggaa     1140

aggacaaugc cgaggcuucc acacugaugg aaaugaaagc cuaucacgcg aucagcagag     1200

cccucgaaaa ggagggccug aaggauaaaa agucuccucu gaaccuuucc cccgaacucc     1260

aggacgagau cgggaccgcu uucagccugu uuaaaaccga ugaggacauu acaggacgcc     1320

ugaaggaucg gauccagccu gaaauucucg aggcccugcu uaaacauauc ucuuucgaca     1380

aguucgucca aauuucccug aaggcucuca gacggaucgu gccccugaug gaacagggca     1440

aacgcuacga ugaggccugc gcggagaucu acggcgacca cuaugggaag aaaaauacgg     1500

aagagaagau uuaccugccc ccuaucccgg ccgaugaaau uagaaacccc gugguccuuc     1560

gggcucucag ccaggccaga aaaguuauca acggaguggu gcgccgguau ggcuccccug     1620

cucgcauuca caucgagacc gccagagaag ucgggaaguc uuuuaaggac agaaaagaga     1680

ucgagaagcg gcaagaagag aaucgcaaag acagagaaaa ggccgcugcg aaauuccggg     1740

aguacuuucc caacuucgug ggagaaccua agagcaaaga uauucugaag cugcgccucu     1800

augagcagca gcauggcaag ugucuguacu ccgggaaaga gaucaaucuu ggcagacuga     1860

acgaaaaggg auauguugag auugaccacg cccuccccuu uagccggaca ugggaugacu     1920

cuuucaauaa caaagugcug guccugggcu ccgaaaauca gaacaagggg aaucaaaccc     1980

cguacgagua cuuuaacgga aaagauaaua gccgcgaaug gcaggaguuc aaggcuagag     2040

uggagacauc ucgguuuccu cgcagcaaga aacagagaau ccuucuccag aaguucgacg     2100

aagauggcuu uaaagagcgg aaccugaaug acaccagaua uguuaaccgc uuccugugcc     2160

aauuuguggc cgaucggaug agacucacgg ggaagggcaa aaagcgcguc uucgccucca     2220

auggacagau uacaaaccug cuucgggggu ucuggggccu gagaaaggug cgcgcugaaa     2280

acgaccggca ucacgcccuc gaugcggucg ugguugcuug uucuaccgug gccaugcagc     2340

agaaaaucac cagauuuguc cgcuacaagg agaugaaugc cuucgacgga aaaacaauug     2400

auaaggagac cggcgaagug cugcaucaga aaacacacuu uccccagccu ugggaguucu     2460

uugcucagga aguuaugauc cggguguucg ggaagcccga cggaaagccu gaguuugaag     2520

aggccgacac gcccgagaaa cugagaaccc uucucgcgga aaagcugagc ucccggccgg     2580

aggcugucca cgaauaugug acaccucugu ucgugagccg cgcccccaac agaaaaaugu     2640

cuggccaggg gcauauggag accguuaagu ccgccaaacg gcucgaugaa ggagucagcg     2700

ugcugcgcgu gccccuuaca caacugaagc ucaaagaccu ggagaagaug gucaauagag     2760

agcgggaacc uaagcuguac gaggcucuua aagccagacu ggaagcucac aaggaugacc     2820

cggccaaagc guuugccgag cccuucuaua aguacgauaa agcuggcaac cgcacccaac     2880

aggugaaggc cguucgggug gaacaagucc agaagacggg gguguggguu agaaaucaua     2940

acggcaucgc ugacaaugcc accauggugc gcgucgaugu guuugagaaa ggagacaagu     3000

auuaccucgu uccuauuuac ucuuggcaag uggccaaagg cauccugccc gaucgggcug     3060

ucgugcaggg gaaggacgag gaagauuggc agcucauuga cgauuccuuc aacuuuaaau     3120

ucagccugca cccuaaugac cuuguggagg ucaucacaaa gaaggcgaga auguucggau     3180

auuuugccuc cugccaccgc ggcaccggga acauuaauau ccggauccau gaccuggauc     3240

acaaaauugg aaagaacggc auccucgaag ggauuggcgu uaaaacagcu cugucuuucc     3300

agaaguacca gaucgacgag cugggaaaag agauuagacc cugucgccuu aagaagagac     3360

cucccgugcg gaaacgcccg gccgccacca agaaagcugg ccaggccaag aaaaagaagu     3420

aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa gagauccuuc     3480

uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau aauaaaucug     3540

guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa agaauu                                          3626


<210>  4773
<211>  3632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4773
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa guaccauguu ggaauugacg ugggcaccuu cagcgugggg cuggcggcua      180

ucgaggucga ugacgccgga augccgauua aaacacucuc ccuggugucu cacauccaug      240

auagcggccu ugaccccgau gaaauuaagu ccgccguuac gcggcuggcu ucuagcggga      300

ucgcccgcag aacccggcgc cucuauagac ggaagcgcag acggcugcag caacuugaca      360

aauuuaucca gcgccaggga uggccuguga uugagcugga agauuacucc gacccccucu      420

auccuuggaa ggucagagcu gagcuggccg cgucuuacau cgccgaugaa aaagagcggg      480

gcgagaagcu gagcguggcu cuccgccaca uugccagaca ucggggcugg cgcaaccccu      540

augcuaaagu uuccagccug uaccuuccug acgggccguc ugaugccuuc aaggcgauca      600

gagaagagau uaagcgggcc uccggacagc ccgugccuga aacagcuacc gucggccaaa      660

uggugacacu gugcgagcuc gggacccuga aacuuagagg agagggcggg gugcugagcg      720

cccgccucca gcagucugac uaugcucggg aaauccagga gauuuguaga augcaagaaa      780

ucggacagga gcuguaccgc aagaucauug augucguuuu ugccgccgaa ucccccaaag      840

gcagcgcuuc uucccgggug ggcaaggacc cucugcagcc cgggaaaaau agagcgcuca      900

aggccagcga cgcuuuccag cgcuaccgga ucgccgcccu gauuggaaac cuuagagugc      960

gcgucgaugg cgagaaacgg auccuguccg uggaggaaaa gaaucucguu uuugaccacc     1020

uggugaaccu uacaccgaag aaagagccug aaugggucac cauugcugag auccugggga     1080

ucgauagagg acaacucauu ggcacggcca ccaugacaga cgauggggaa cgcgcgggag     1140

cucggccccc uacccacgac acaaauagau cuaucgugaa cagccggauu gcuccccugg     1200

uugauuggug gaagaccgcc uccgcccugg agcagcaugc cauggugaaa gcucucagca     1260

augccgaggu cgacgauuuc gacucucccg aaggcgcgaa ggugcaggcc uuuuucgcug     1320

aucuggacga cgauguccac gccaaacuug acucccugca ucucccugug ggccgcgcug     1380

ccuauagcga ggauacgcug guuagacuua cacggagaau gcugucugac gggguggauc     1440

ucuacaccgc gcgccugcaa gaauuuggaa ucgagccgag cuggacaccc ccuacacccc     1500

ggauuggcga accugucggg aaccccgccg uggacagagu ucugaagacc gugucccgcu     1560

ggcucgaguc ugcuaccaag acguggggag ccccggagag agucaucauc gaacacgugc     1620

gggagggcuu cgugaccgaa aaacgcgcua gagagaugga uggggacaug cggcgcagag     1680

ccgcccggaa ugcuaagcug uuucaggaga ugcaggaaaa acuuaacguu cagggcaagc     1740

cuagccgcgc ggaucugugg agauaucaau ccguccagcg gcagaauugc cagugugccu     1800

acugcggaag ccccauuaca uucucuaacu ccgagaugga ccacaucgug ccucgcgcug     1860

gccaagggag caccaauaca agagaaaacc ucguggccgu cugucaucgg ugcaaucagu     1920

cuaaaggaaa cacccccuuu gccauuuggg cuaagaacac guccaucgag ggcgugagcg     1980

uuaaggaagc cguggagaga acacgccacu gggucaccga uaccgggaug cgguccacag     2040

acuucaaaaa guucaccaaa gcggugguug agagauuuca gcgcgcuaca auggaugaag     2100

agauugacgc ccggucuaug gaaagcgugg ccuggauggc uaaugagcug agaucccgcg     2160

ucgcccagca uuucgcgucu cacggaacga ccgugcgggu uuauagaggc agccugacag     2220

cugaagcccg ccgggccucc gggaucagcg gcaagcugaa auuuuucgac ggagugggca     2280

agucuagacu cgaucggcgc caucacgcua uugacgccgc ugucaucgcc uuuaccuccg     2340

auuacguggc ggagacacug gccgugagaa gcaaccugaa gcaaucucag gcucaccggc     2400

aggaggcccc ucaguggcgc gaauuuaccg ggaaagacgc ugagcauaga gccgccuggc     2460

gggucuggug ucaaaagaug gaaaaacucu ccgcucugcu uacggaggau cugagagacg     2520

aucgcguugu ggugaugagc aauguccggc ucagacuggg aaacggcucu gcgcacaagg     2580

aaaccaucgg gaaacugagc aaggugaaac uuuccagcca gcucucuguu uccgacauug     2640

auaaggccag cuccgaggcu cuguggugcg cccugacacg cgagcccgga uuugacccga     2700

aggaaggccu cccugccaau cccgagcggc auaucagagu gaacgggacc cacgucuaug     2760

cuggcgauaa uauuggacug uuccccgugu cugccggcag caucgcgcuu cgcgggggau     2820

acgcugaacu gggcuccucu uuucaccaug cccggguuua caaaauuaca agcgggaaga     2880

aaccugccuu cgcuaugcuc agaguguaua ccaucgaccu gcugccguac cgcaaccagg     2940

aucuuuucuc cgucgagcuc aagccccaaa cgaugucuau gagacaggcc gagaaaaagc     3000

ugcgggacgc gcuggcuaca ggaaaugccg aauaucucgg cuggcuggug guugacgaug     3060

agcuuguggu cgacaccagc aagaucgcca cagaucaggu gaaagcugug gaagccgagc     3120

uggggaccau ucgcagaugg cgggucgacg gcuucuuuuc cccuagcaag cuccgccuga     3180

gaccccugca gaugucuaaa gaaggaauca agaaagaguc cgcuccugag cuuagcaaga     3240

uuaucgaucg gcccggcugg cucccugccg uuaacaagcu guucucugac gggaauguga     3300

ccguggucag acgcgauucc cugggacggg ugagacucga aagcacagcg caccuucccg     3360

uuacguggaa agugcaaaag cgcccggccg cuaccaaaaa ggccggccag gcuaaaaaga     3420

agaaauaagg acuaguaaua aggaaacugu augaaugucu gugggcagga agugaagaga     3480

uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua     3540

aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3632


<210>  4774
<211>  3332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4774
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc gcggauucug gcuuucgaca ucggaauuag cuccaucggc ugggccuuuu      180

cugagaacga ugaacucaag gacugcgggg uucgcauuuu caccaaagug gagaauccga      240

agacaggaga aagccuggcc cuucccagac ggcuggcucg cuccgccaga aagcggcucg      300

cucgcagaaa agcccggcug aaccaucuua agcaccugau cgcgaaugag uuuaaacuca      360

acuacgaaga uuaucagucu uucgacgaga gccuggccaa ggcuuacaaa ggcucccuga      420

ucucuccuua ugagcuccgc uuuagagccc ugaaugaacu ucugagcaag caagauuucg      480

cucgggugau ucuccauauc gccaagcgca gaggguacga cgauauuaaa aacuccgacg      540

auaaggagaa aggagcgauc cugaaggcca uuaaacagaa ugaagagaaa cuugcuaacu      600

aucagagcgu cggcgaguac cuguauaaag aauacuuuca gaaguucaag gaaaauucua      660

aagaauuuac gaacgugcgg aauaagaaag aguccuacga acgcuguauc gcccagagcu      720

uccucaagga cgagcugaaa cugauuuuua agaagcaaag agaguucggc uuuucuuucu      780

ccaaaaaguu cgaagaggaa guucucagcg uggcuuuuua uaaacgggcc cugaaggauu      840

ucucucaccu ugucgggaac ugcuccuuuu ucaccgacga gaaaagagcc cccaagaaua      900

gcccucuggc uuuuauguuc guggcgcuca cacgcaucau caaccugcuu aauaaccuga      960

agaacaccga aggcauucuc uacacaaaag augaccugaa ugcccugcuc aacgagguuc     1020

ugaagaaugg aacccuuaca uauaaacaga ccaagaaacu gcucgggcug uccgacgauu     1080

acgaguuuaa gggagaaaag ggcacguauu ucaucgaguu uaaaaaguac aaagaguuca     1140

uuaaggcucu uggggagcau aaccugucuc aggacgaucu caaugaaauc gccaaagaca     1200

uuacccugau caaggaugag aucaaacuga agaaggcccu cgcuaaauau gaccugaacc     1260

aaaaucagau ugauagccuu uccaagcugg aguuuaaaga ccaccucaac aucagcuuca     1320

aggcccugaa acuggugaca ccccuuaugc ucgaaggaaa gaaguacgau gaggcgugua     1380

augaacugaa ccugaaaguc gcuauuaaug aggacaagaa agauuuucuc ccugccuuca     1440

acgagaccua cuauaaggac gaagugacaa auccgguggu ccugcgggcc aucaaagagu     1500

acagaaaggu ucuuaacgcu cugcucaaga aauauggcaa ggugcacaaa auuaauaucg     1560

aacuggcccg cgaggugggc aagaaccauu cucagcgggc uaaaaucgaa aaggagcaga     1620

acgagaauua caaggccaaa aaggacgcgg aacuggagug cgaaaaacuu gggcucaaga     1680

uuaacuccaa aaauauccug aagcugagac ucuucaaaga gcaaaaggaa uuuugugccu     1740

auagcggaga gaagauuaaa aucucugauc ugcaggacga gaagaugcuu gaaauugauc     1800

acaucuaccc cuauagccgc uccuucgacg auucuuacau gaacaaaguc cugguguuua     1860

ccaagcagaa ucaagagaaa cucaaccaga cgccuuucga ggcuuuuggc aaugacagcg     1920

ccaaguggca gaagauugaa guucuggcua aaaaccugcc cacaaagaaa cagaagcgga     1980

uccuugauaa aaauuacaag gacaaggaac aaaagaacuu caaggauaga aaucucaacg     2040

acacccgcua uaucgcccgg cuggugcuga auuacacaaa agauuaucuc gacuuucugc     2100

cucuuuccga ugacgagaac accaagcuga augacaccca gaaagggagc aagguccaug     2160

uggaggcgaa gucuggaaug cucacauccg cccugagaca cacguggggc uucagcgcua     2220

aagaucgcaa caaccaucug caccacgcca uugacgcugu uaucauugcc uacgccaaua     2280

acucuaucgu gaaggcuuuu uccgauuuca aaaaggaaca ggagagcaau uccgcggaac     2340

uuuaugccaa aaagauuucu gagcucgacu acaagaacaa acggaaguuu uucgagcccu     2400

uuagcggguu cagacagaaa guccuggaua agaucgacga aaucuucgug uccaaaccgg     2460

agcggaagaa accuucugga gcucugcaug aagagaccuu ucgcaaggaa gaggaguucu     2520

aucaaagcua cggcggcaag gaaggggucc ucaaagcccu ggagcuugga aagauuagaa     2580

aagugaaugg caagaucguu aaaaacgggg auauguuucg gguggacauu uucaagcaca     2640

agaaaacaaa uaaguuuuac gccgucccca ucuauaccau ggauuucgcu cugaaagugc     2700

ucccuaacaa ggccguugcg agauccaaaa agggagaaau uaaggacugg auccugaugg     2760

augagaauua cgaauuuugc uucagccugu auaaagacuc ucuuauccug auucagacaa     2820

aggauaugca ggagcccgag uuuguguacu auaacgcuuu caccuccagc acggucucuc     2880

ucaucguguc caaacaugac aauaaguuug aaacacugag caaaaaccag aagauucucu     2940

ucaagaaugc caacgagaaa gaagugaucg ccaagucuau uggcauccaa aaucugaaag     3000

uuuucgagaa guacaucguc agcgcucuug gggaagugac caaagccgag uuucgccagc     3060

gggaggacuu caagaaaaag agacccgcgg cuaccaagaa agccggccag gccaagaaaa     3120

agaaauaagg acuaguaaua aggaaacugu augaaugucu gugggcagga agugaagaga     3180

uccuucugua aagguuuuug gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua     3240

aaucugguag aaaaaugaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3300

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3332


<210>  4775
<211>  3743
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4775
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccag cgaccugguu cucggacugg auauuggcau cggguccgug ggagugggca      180

uucuuaacaa ggucaccggg gagaucauuc auaaaaauuc ucggaucuuc ccggcggcuc      240

aggccgaaaa caaucuggug cgcagaacaa accggcaagg acgcagacuc gcccggcgca      300

agaagcacag acggguucgc cugaauagac uuuuugagga aagcggccug aucacggacu      360

ucaccaaaau uuccaucaac cucaaucccu accagcugcg ggugaagggc cugacagaug      420

agcucucuaa cgaggagcug uuuauugcuc uuaaaaauau ggucaagcau cgcgggauca      480

gcuaucugga cgaugccucc gacgauggaa acucuagcgu gggcgacuac gcucagauug      540

uuaaagaaaa uuccaagcag cucgaaacca agacaccugg gcaaauccag cuggagagau      600

aucagaccua cggacagcuu cggggcgauu ucacagugga aaaagacggg aagaaacacc      660

gccugauuaa cgucuuuccc accagcgccu auagaucuga ggcgcuccgg auccugcaaa      720

cgcagcagga guucaauccu cagaucaccg augaauuuau uaacagauac cuggagaucc      780

ucacaggaaa gcgcaaauau uaccauggcc ccggcaauga aaagucccgg accgacuacg      840

ggagauaucg cacaagcgga gagacccugg auaacauuuu cggcauccuu auugggaaau      900

gcacguuuua cccugacgag uuccgggccg cuaaggccuc uuauacagcu caagaauuua      960

accugcucaa ugaccugaac aaucuuaccg ugccgacaga gaccaagaaa cuguccaagg     1020

aacagaaaaa ccagaucauc aauuacguga agaacgagaa agccauggga cccgcgaaac     1080

ucuucaagua uauugccaaa cugcugagcu gugaugucgc ugacaucaag ggcuacagaa     1140

uugauaaauc ugggaaggcc gaaauccaca ccuuugaggc uuaucgcaaa augaagacac     1200

ucgaaacgcu ggacauugag caaauggauc gggagacccu ugacaagcug gccuacguuc     1260

ucacacugaa uaccgaaaga gagggaaucc aggaagcccu ugagcacgag uucgcugaug     1320

gcuccuucag ccagaaacag guggacgaac uggugcaauu ucgcaaggcg aacuccucua     1380

ucuucggcaa aggguggcau aauuuuagcg ucaagcucau gauggagcug auuccugaac     1440

uguacgagac auccgaggaa cagaugacca uccucacgcg gcugggaaaa cagaagacaa     1500

ccucuucuuc caacaagacc aaauauauug augagaaacu ucugacagaa gagaucuaca     1560

aucccguggu ugccaagagc gugagacagg cuauuaagau cgucaacgcc gccauuaaag     1620

aauauggcga cuucgauaau aucgugaucg agauggcucg cgaaaccaac gaggacgacg     1680

agaagaaagc cauucaaaag auccagaagg cgaauaaaga ugaaaaggac gcugccaugc     1740

ucaaagccgc uaaccaguac aacgggaagg ccgagcugcc ucacucuguu uuucauggac     1800

acaaacagcu ggcuacaaag auucggcuuu ggcaccaaca gggcgaaaga ugccucuaua     1860

cggggaagac caucagcauu caugaucuga ucaauaacuc caaucaguuc gagguggacc     1920

acauccugcc ccucucuauu acauuugaug acagccuggc caacaaaguc cuuguguacg     1980

cgaccgccaa ucaggaaaag ggccaacgga caccguauca ggcucuggau uccauggacg     2040

augccuggag cuuccgcgag cucaaggcuu uugucagaga gucuaagacc cuguccaaca     2100

aaaagaagga auaccugcuu acggaggaag acaucagcaa auucgaugug cggaagaaau     2160

uuauugagag aaaucucguu gacacccgcu acgccucucg ggugguccug aacgcgcugc     2220

aggaacauuu cagagcccac aagaucgaua caaaaguguc cguugugcgc ggacaauuua     2280

ccagccagcu cagacggcau uggggcauug agaagacacg cgacaccuau caccaccaug     2340

cugucgacgc ccugaucauc gcugccuccu cucagcuuaa ucuguggaag aaacagaaga     2400

acacgcucgu gagcuacucc gaggaucaac ugcuggacau ugaaacaggg gagcuuaucu     2460

cugaugacga auauaaagag agcguguuca aggccccuua ccagcacuuc guugauaccc     2520

ucaaauccaa ggaguuugaa gacagcauuc uguucucuua ucaggucgau uccaaauuua     2580

auagaaagau cagcgacgcu acaauuuacg cgacccggca ggccaaggug ggaaaagaua     2640

aggcugacga gaccuaugug cugggcaaaa ucaaggauau cuacacacaa gacggguacg     2700

augccuucau gaaaauuuau aagaaggaca aaucuaaguu ucucauguac cgccaugacc     2760

cccagacguu cgaaaaaguc aucgagccua uucuggaaaa cuaucccaau aagcagauca     2820

acgagaaagg aaaggaggug cccuguaauc cuuuucuuaa guacaaagag gaacacggcu     2880

auauuagaaa guacuccaaa aaggggaacg gcccggaaau caaaagccug aaguauuacg     2940

auucuaagcu cggaaaccac auugacauca cccccaaaga uagcaauaac aagguugugc     3000

ugcaguccgu cagcccuugg cgggccgacg uguacuucaa uaaaacaacc ggcaaguaug     3060

agauccuggg gcuuaaauac gcugaucucc aauuugaaaa gggaacaggc accuauaaga     3120

uuucucagga gaaauacaac gacaucaaga aaaaggaggg gguugauucc gacagcgagu     3180

ucaaauuuac gcuguauaag aaugaucugc uccuggugaa agacacagaa accaaggaac     3240

agcaacuuuu ccgcuuucug uccagaacca ugcccaagca gaaacauuac gucgagcuca     3300

agccuuauga uaaacagaag uucgaaggag gcgaggcccu gauuaaagug cuggggaacg     3360

uugcgaauag cggccagugc aagaagggac uuggcaaaag caacaucucc auuuacaagg     3420

ugcggacaga cguccugggg aaucaacaca ucaucaaaaa cgagggagac aagcccaaac     3480

ucgauuucaa gcgcccggcu gccaccaaga aagccggcca ggcuaagaaa aagaaauaag     3540

gacuaguaau aaggaaacug uaugaauguc ugugggcagg aagugaagag auccuucugu     3600

aaagguuuuu ggaauuaugu cugcugaaua auaaacuuuu uugaaauaau aaaucuggua     3660

gaaaaaugag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaga auu                                             3743


<210>  4776
<211>  4301
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4776
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccac ccaguucgag ggauuuacaa accuguacca aguuagcaag acgcuccggu      180

ucgaacugau uccgcagggc aaaacccuua agcauaucca ggagcagggg uuuauugaag      240

aggacaaggc gcgcaaugau cacuauaaag aacugaagcc caucauugac agaaucuaca      300

aaacauaugc ugaucaaugc cuccagcugg ugcagcuuga cugggagaac cuguccgccg      360

ccaucgauuc uuaccggaag gagaaaaccg aagagacacg caaugcucuc auugaagaac      420

aggccaccua uagaaacgcu auccaugacu acuucauugg acggacagau aaucugaccg      480

acgccaucaa caagcgccac gcggagauuu auaagggccu guuuaaagcc gaacucuuca      540

augggaaggu gcugaaacaa cuuggaacgg ucaccacaac cgagcaugaa aacgcucugc      600

ucagauccuu ugauaaguuc acaaccuacu uuagcggcuu cuacgagaau cggaaaaacg      660

uguuuucugc cgaagacauc agcacggcua uuccucaccg caucguucag gauaauuucc      720

ccaaguuuaa agagaacugu cacaucuuca caagacugau uaccgccgug ccuucccuuc      780

gggagcauuu cgaaaauguc aagaaggcga ucggcauuuu ugugucuaca agcaucgagg      840

aaguuuucuc cuuucccuuc uauaaccagc ugcucaccca gacccaaauu gaccuguaca      900

aucagcugcu cgggggaauc agccgcgagg ccggcacaga gaaaaucaag gggcugaacg      960

aggugcuuaa ccuggcuauu cagaaaaaug augagacggc ccacaucauu gcuucucucc     1020

cucauagauu uaucccgcug uucaagcaaa uucuuuccga ccggaacacc cugagcuuua     1080

uccucgaaga guucaaaucu gacgaagagg ucauccaguc cuuuugcaag uauaagacac     1140

ugcugcgcaa ugaaaacgug cucgagaccg ccgaggcccu guucaaugaa cuuaacagca     1200

uugaucugac acacaucuuu auuucucaca aaaagcucga gaccaucucc agcgcucugu     1260

gugaccauug ggauacgcuu agaaaugcgc uguacgaacg gagaauuucc gagcucacag     1320

gaaaaaucac caagucugcc aaagagaagg ugcagcgcag ccugaagcac gaagacauua     1380

accugcagga gaucaucucc gcugccggca aagaacucag cgaggccuuc aagcagaaaa     1440

ccucugaaau ucugucccau gcucacgccg cgcuugauca accccugccu acaacccuca     1500

agaaacagga ggagaaggaa auccuuaaga gccagcugga cucucuucuc gggcuguauc     1560

aucugcucga uugguuugcu gucgacgaga gcaaugaagu ugaucccgag uucuccgccc     1620

ggcugacagg aauuaaacuu gaaauggagc cuucucugag cuucuacaac aaggccagaa     1680

auuaugcuac gaaaaagccc uacuccgugg agaaauuuaa gcucaacuuc caaaugccga     1740

cccuggccag cggcugggac gugaauaaag aaaagaacaa uggcgcuauc cuguuuguca     1800

agaacgggcu uuacuaucuc ggaauuaugc cuaaacagaa gggccgcuac aaagcccugu     1860

cuuucgagcc cacagaaaag accuccgagg gguuugauaa aauguauuac gacuauuucc     1920

cugacgcggc caagaugauc cccaagugca gcacacagcu gaaagcugug accgcccacu     1980

uucagacgca caccacaccc auccuccugu cuaacaauuu cauugaaccu cuugagauca     2040

ccaaggagau uuacgaucug aacaauccgg aaaaagagcc caagaaauuu caaacagcuu     2100

augccaagaa gaccggagac cagaaaggcu accgggaagc gcucuguaag uggaucgauu     2160

ucacgagaga cuuucugucc aaauacacaa agaccacaag cauugaucug uccucucuuc     2220

gcccuagcuc ccaguauaaa gaccucgggg aguacuaugc cgagcugaac ccccugcucu     2280

accauaucuc uuuccagcgg aucgcugaaa aggagauuau ggaugccguu gaaaccggaa     2340

agcuguaucu uuuucaaauc uacaauaaag acuucgcuaa gggccaccau ggcaaaccua     2400

accugcacac ccucuauugg acagggcugu ucagccccga gaaucuggcc aagacgucca     2460

uuaaacuuaa cggacaggcc gaacucuuuu acagaccuaa gagccgcaug aaacggaugg     2520

cucacagacu gggcgagaag augcugaaua agaaacucaa ggaucagaaa accccgaucc     2580

ccgacacacu guaccaagag cuuuaugauu acgugaacca ucgccugucu cacgaccucu     2640

ccgaugaagc gcgggcccug cugccuaaug ucauuaccaa ggaggugagc caugaaauca     2700

ucaaagacag acgguucaca ucugacaagu uuuucuuuca cguucccauu acccuuaacu     2760

aucaggcugc caauuccccu agcaaguuca accagcgcgu gaaugccuac cugaaagagc     2820

aucccgaaac gccgaucauu gggaucgaua gaggagagcg gaaccucauu uauaucacag     2880

ucauugacuc uaccggcaag auccuggagc agagaagccu caacaccauc caacaguuug     2940

auuaccagaa aaagcuggac aaucgcgaaa aagagcgggu ggcugccaga caggcguggu     3000

ccgucguggg gacaauuaag gaucuuaagc aaggcuaucu gagccagguu auccacgaaa     3060

uuguggaccu caugauccac uaccaggcug ucgugguucu ggagaaccug aauuucggau     3120

uuaaaucuaa gcgcaccggc auugccgaaa aagccgugua ccagcaauuc gagaagaugc     3180

uuaucgauaa acugaacugc cucguccuga aggacuaucc ugcugagaag guugggggag     3240

ugcucaaucc cuaccagcug acagaucagu uuacguccuu cgccaaaaug ggcacccaaa     3300

gcggguuccu guuuuaugug cccgcgccuu acacauccaa gaucgacccg cugaccggau     3360

ucgucgaucc cuuugugugg aaaacaauua agaaccauga aucuagaaaa cacuuccucg     3420

agggcuuuga cuuccugcau uaugauguga agaccgggga cuuuauccug cacuucaaga     3480

ugaaucggaa ccuuagcuuu cagcgcggcc ugccuggauu caugcccgcu ugggacauug     3540

ucuuugagaa aaaugaaacg caguucgaug ccaagggcac cccuuuuauc gccgggaaaa     3600

gaauugugcc cguuaucgag aaccaucggu ucacaggacg cuacagagac cucuauccug     3660

cuaaugaacu gaucgcccuc cuggaggaaa agggcauugu guuccgggau ggguccaaca     3720

uccuucccaa acugcuggag aaugacgauu cucacgcuau ugacaccaug gucgcccuca     3780

uccgcagcgu gcugcagaug agaaacucca augcggccac aggagaagau uacauuaacu     3840

cuccgguucg ggaccuuaac ggcguguguu uugauagccg cuuccaaaau ccugaguggc     3900

ccauggacgc ugaugccaac ggggcuuacc acaucgcccu gaagggccag cuccugcuca     3960

aucaucugaa agaguccaag gaccuuaagc ugcagaacgg aauuagcaau caggacuggc     4020

uggccuauau ccaagaacuc agaaacaaac ggccugcugc gaccaagaaa gccggccagg     4080

cuaagaaaaa gaaguaagga cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa     4140

gugaagagau ccuucuguaa agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu     4200

gaaauaauaa aucugguaga aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         4301


<210>  4777
<211>  4280
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4777
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccag cauuuaccag gaguucguua acaaguauuc ccugucuaaa acccuccggu      180

uugaacugau cccgcaagga aagacacuug agaauauuaa ggcgcgcggc cugauccucg      240

acgaugaaaa gagagcuaag gacuacaaaa aggccaaaca gauuaucgau aaguaucauc      300

aguucuuuau cgaggaaauu cugagcuccg ugugcaucuc ugaggaccuu cugcagaacu      360

acagcgaugu guauuucaag cucaaaaagu ccgacgauga caaucugcaa aaagauuuua      420

agucugccaa agacacgauu aagaaacaga ucagcgagua cauuaaggau uccgaaaagu      480

ucaaaaaccu guuuaaucag aaccucaucg acgcuaagaa agggcaggag agcgaucuga      540

uucuuuggcu gaagcaaucu aaagacaaug gaaucgaacu cuucaaggcc aacuccgaca      600

ucaccgauau ugacgaggcu cuggagauca uuaagagcuu uaaaggcugg acaaccuauu      660

ucaagggguu ucacgaaaau cggaaaaacg ucuacucuuc caaugauauc cccacaagca      720

uuaucuaccg caucguggac gauaaccuuc cuaaguuccu ggagaauaaa gccaaguaug      780

aaucucucaa ggacaaagcg cccgaggcca uuaacuacga acagaucaag aaagaucugg      840

cugaggagcu gaccuuugac auugauuaua agacauccga aguuaaucag agaguguuca      900

gccucgacga ggucuucgaa aucgccaacu uuaacaauua ccugaaccag uccggaauua      960

ccaaauucaa uacgaucauc ggcggcaagu uugugaacgg ggagaauacc aagcggaaag     1020

gaauuaacga auauaucaau cuuuacucuc aacagauuaa cgauaagaca cugaaaaagu     1080

auaaaaugag cguucucuuc aagcagaucc uguccgacac cgagagcaaa ucuuuuguga     1140

uugacaaacu ugaggaugac uccgaugucg ugacaaccau gcagagcuuc uacgaacaaa     1200

ucgcugccuu uaagacggug gaggaaaaau cuauuaagga gacacugagc cuccuguucg     1260

acgaucugaa agcgcagaag cucgaccugu ccaaaaucua cuuuaagaau gauaagucuc     1320

uuaccgaccu gagccagcaa gucuucgaug acuauuccgu uaucggcaca gccgugcucg     1380

aauacauuac ccagcagauc gcuccuaaaa accuggauaa ucccagcaag aaagagcagg     1440

agcuuauugc caagaaaacc gaaaaggcua aguaucuguc ucucgagaca aucaaacugg     1500

cccuggaaga guuuaacaag caucgcgaca uugauaaaca auguagauuc gaggaaaucc     1560

ucgccaauuu ugcugcgauc ccuaugauuu ucgacgagau cgcccagaac aaggacaauc     1620

uggcucagau uuccaucaaa uaccagaacc aagggaagaa ggaucuucug caggccagcg     1680

ccgaagacga ugugaaagcu auuaaggacc uccuggauca gacgaacaau cugcuucaca     1740

aacucaagau cuuccauauc ucucaguccg aggacaaagc caacauucug gauaaggacg     1800

aacacuuuua ucuggucuuc gaggagugcu acuuugaacu cgcgaauauc gugccgcugu     1860

auaacaaaau ucggaauuac aucacccaaa agcccuacag cgaugagaag uucaaacuua     1920

acuuugaaaa uuccacacug gcuaacggau gggacaagaa uaaagagccu gauaacaccg     1980

ccauucucuu caucaaggac gauaaauauu accugggcgu uaugaauaag aagaacaaua     2040

aaaucuuuga cgacaaggcc auuaaagaaa acaaggggga gggauauaaa aagaucgugu     2100

acaagcugcu ucccggcgcu aauaaaaugc ucccuaaggu cuucuuuucu gccaaaagca     2160

uuaaguucua uaaccccucc gaggauaucc ugcgcauuag aaaccacucu acacauacca     2220

aaaauggcag cccgcagaag ggguacgaaa aguuugaguu caacaucgaa gacugucgga     2280

aauuuauuga uuucuauaag caguccauca gcaaacaccc ugaguggaag gacuucggau     2340

uucgcuucuc ugauacgcaa agauacaauu ccaucgacga auuuuaccgg gagguggaga     2400

accagggcua uaaacugaca uucgaaaaua uuagcgaguc uuacaucgau uccguuguga     2460

accaggggaa gcucuaucug uuucagauuu acaauaagga cuucagcgcu uauucuaaag     2520

gacgccccaa ccuucauacc cuguacugga aggcccucuu ugaugaaaga aaucugcaag     2580

acgucgugua uaaacugaac ggcgaggcgg agcuuuucua ccggaagcag agcaucccua     2640

aaaagauuac ccaccccgcc aaagaagcua ucgccaauaa gaacaaggau aaucccaaaa     2700

aggaguccgu cuuugaauac gaccucauca aagacaagag auucacagag gauaaauuuu     2760

ucuuccacug cccuauuacc aucaacuuua agagcucugg ggcuaauaag uucaacgacg     2820

aaauuaaccu gcugcucaaa gagaaggcca augaugugca uauccugucc auugaccgcg     2880

gagagcggca ccuugcguau uacacacugg uugauggcaa aggcaacauc aucaagcagg     2940

acacguuuaa uauuaucggg aacgauagaa ugaaaaccaa uuaucaugac aagcucgccg     3000

cuauugaaaa ggaucgcgac agcgcccgga aagauuggaa gaaaaucaac aauauuaagg     3060

agaugaaaga aggauaccug ucccaggugg uccacgagau cgcuaagcug gugauugaau     3120

auaacgccau cguuguguuc gaggaccuua auuuuggcuu caagagaggg cgcuuuaaag     3180

ucgagaagca aguguaccag aaacucgaaa agaugcugau cgagaaacug aacuaucucg     3240

uguucaagga uaaugaauuu gacaaaacag gaggcguucu gcgggccuac cagcuuaccg     3300

cuccguucga gacauuuaag aagaugggga aacagaccgg cauuaucuac uauguccccg     3360

cgggauucac gucuaagauu uguccuguga ccggcuuugu gaaccaacug uaccccaaau     3420

augaaagcgu cuccaagucu caggaguucu ucagcaaauu ugacaagauc ugcuacaauc     3480

ucgauaaggg guauuucgag uuuuccuucg acuacaaaaa cuuuggagau aaggccgcua     3540

aaggcaagug gacaauugcc ucuuucggga gcagacugau caauuuucgc aacuccgaca     3600

aaaaucauaa cugggauacc cgggaagugu auccuacaaa ggagcuggaa aaacuucuga     3660

aagacuacag caucgaguac ggacacggcg aguguauuaa ggccgcuauc ugcggggaau     3720

cugauaaaaa guucuuugcc aaacucaccu ccguucugaa cacgauucuc cagaugagaa     3780

auagcaagac aggcaccgag cuggacuauc uuaucucucc cguggcggau gucaacggaa     3840

auuucuuuga cucccgccaa gcuccgaaga acaugccuca ggaugccgac gccaauggcg     3900

cuuaccacau ugggcugaaa ggacucaugc ugcugggccg gaucaagaac aaucaggaag     3960

ggaaaaagcu gaaccuugug aucaaaaaug aggaauauuu cgaguuuguu cagaacagaa     4020

auaacaagcg gcccgccgcg acaaagaagg cuggacaagc caagaaaaag aaauaaggac     4080

uaguaauaag gaaacuguau gaaugucugu gggcaggaag ugaagagauc cuucuguaaa     4140

gguuuuugga auuaugucug cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa     4200

aaaugagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaagaauu                                                 4280


<210>  4778
<211>  4581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4778
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgacaa gaaauacagc      240

auuggacugg auaucggcac caacuccguu gggugggcgg ugauuacaga cgaguauaag      300

gugccgucua agaaauucaa gguccucgga aauacggauc ggcauagcau caaaaagaac      360

cugauuggcg cucugcuguu ugacuccggg gaaaccgccg aggccacacg ccucaaaaga      420

accgcucggc gcagauacac acggcgcaag aauagaaucu gcuaucugca ggaaaucuuc      480

ucuaacgaga uggccaaggu ggaugacagc uuuuuccacc ggcuugaaga guccuuucug      540

guugaggaag auaaaaagca ugagcgccac cccauuuucg gaaauaucgu ggacgaaguc      600

gcuuaccaug agaaauaucc uaccauuuac caccucagaa agaaacuggu ggauucuaca      660

gacaaggccg aucugcggcu caucuaucug gcgcuugccc acaugauuaa auuucgcggc      720

cauuuccuga ucgagggcga ccucaacccc gauaauagcg acguugacaa gcuguuuauu      780

caacuugugc agaccuacaa ccagcuguuc gaagagaauc cuaucaacgc uuccgggguc      840

gaugccaagg cuauccucag cgccagacug ucuaaauccc ggcgccugga aaaucucauu      900

gcgcagcugc ccggagagaa gaaaaacggc cuuuuuggga aucugaucgc ccucagccug      960

ggacuuacgc cuaacuucaa gucuaauuuu gaccuggcug aagaugccaa acuccaacug     1020

uccaaggaca ccuacgauga cgaucuggac aaccuccugg cucagauugg cgaucaguau     1080

gccgaccuuu uccuggccgc uaagaaccuc agcgaugcga uccugcuuuc ugacauucug     1140

agagugaaua cagagaucac caaagccccg cucuccgcua gcaugaucaa gcgguacgac     1200

gagcaccauc aggaucugac acugcucaaa gcccugguga gacaacagcu ucccgaaaag     1260

uauaaagaga uuuucuuuga ccaguccaag aacggguacg ccggauauau cgauggcggc     1320

gcuucucagg aagaguucua caaguuuauu aaaccuaucc uggaaaagau ggacgggacc     1380

gaggagcucc uggucaaacu gaaucgcgaa gaucuucucc ggaagcaaag aacguucgac     1440

aacggaagca uuccccacca gauccaccug ggcgagcugc augccauccu ccgccggcag     1500

gaagauuuuu auccuuuccu gaaagacaau agagagaaga uugaaaagau ccuuacauuu     1560

cgcauucccu acuacguugg gccgcuggcg cggggaaacu ccagauucgc uuggaugacc     1620

cgcaaaagcg aggagacaau caccccuugg aauuuugaag agguggugga uaagggcgcc     1680

ucugcccaau ccuucauuga acggaugacc aacuuugaca aaaaucuccc caacgagaag     1740

guccugccua aacacagccu gcuuuaugag uacuucacag uguauaauga acucacgaag     1800

guuaaauacg ugaccgaggg gaugagaaag cccgcuuuuc ugucuggaga acagaagaaa     1860

gccaucgucg aucuucucuu caagacaaac cgcaaaguga ccguuaagca gcugaaagag     1920

gacuauuuca agaagauuga auguuuugau agcguggaga ucuccggcgu cgaggaccgg     1980

uucaaugcuu cucuuggcac auaccaugac cugcucaaaa ucauuaagga uaaagacuuu     2040

cuggauaacg aagagaauga agacauccug gaggauauug ugcuuacccu cacgcuguuc     2100

gaagacagag agaugaucga ggaacggcug aagacauaug cccaccucuu ugaugacaaa     2160

gucaugaagc agcugaagcg cagacgguac accggguggg gaagacuuag ccgcaaacug     2220

auuaacggca uccgggauaa gcaauccggg aaaaccaucc ucgacuuccu uaagagcgau     2280

ggauuugcga acagaaauuu caugcagcug auucaugacg auucucugac auuuaaagag     2340

gacauccaga aggcccaggu guccggccaa ggggacagcc uccacgaaca cauugcuaac     2400

cuggccggcu cucccgcuau caagaaaggc auucugcaga ccguuaaggu ggucgaugag     2460

cucgugaaag uuaugggacg ccauaagccu gaaaauaucg ugaucgagau ggccagagag     2520

aaccagacaa cgcagaaagg gcaaaagaac ucccgggaac gcaugaaaag aauugaggaa     2580

ggaaucaagg agcugggcag ccagauucuu aaggagcacc cggucgaaaa uacccagcug     2640

cagaaugaga aacucuaccu guauuaccug caaaacgggc gggacaugua uguggaucag     2700

gaacuugaca ucaaucgccu guccgauuac gacguggauc auauuguucc ccagucuuuc     2760

cucaaggacg auagcaucga caacaaaguc cugacaagau ccgauaagaa ucggggaaaa     2820

ucugacaacg ugccuagcga ggaagugguc aagaagauga aaaauuauug gcgccaacuc     2880

cugaacgcga aacuuaucac ccagagaaaa uuugacaauc ugacaaaggc cgagcggggc     2940

gggcucuccg agcuggauaa agcuggcuuc auuaagcgcc agcuggugga aaccagacag     3000

aucacgaagc acguugccca aauucuugac agccggauga acaccaaaua cgaugagaac     3060

gacaagcuga ucagagaagu gaaagucauu acacucaagu cuaaacuggu guccgauuuu     3120

cgcaaggacu uccaguucua uaagguucgg gagaucaaua acuaccacca ugcucacgau     3180

gccuaccuca augccguggu cggaaccgcu cugauuaaaa aguaucccaa acuugaaagc     3240

gaguuugugu acggcgacua uaagguuuac gaugugagaa aaaugaucgc gaagucugag     3300

caggaaaucg ggaaggccac agcuaaauau uucuuuuacu ccaacauuau gaauuucuuu     3360

aagaccgaga ucacgcuggc caacggagaa auucgcaaac ggccucucau cgagacaaau     3420

ggcgaaaccg gggagauugu cugggacaag ggaagagauu ucgccacagu gcgcaaagug     3480

cugagcaugc cccaggucaa caucguuaag aaaaccgagg ugcaaaccgg cggguuuucu     3540

aaggaaagca uccugccgaa gagaaauucc gacaaacuua uugcucggaa gaaagauugg     3600

gacccuaaga aauauggcgg auucgacagc cccacagugg ccuacucugu ccuggugguu     3660

gcgaaggugg agaagggcaa auccaagaaa cucaagagcg ucaaagaacu gcucgggauc     3720

acgauuaugg agcgcuccuc uuuugagaag aacccuaucg auuuccugga agcuaaggga     3780

uacaaagagg ugaagaaaga ccuuauuauc aagcugccca aauauagccu guuugaacuc     3840

gagaauggcc ggaagagaau gcuggccucc gccggggaac uucagaaggg aaacgagcug     3900

gcucucccuu cuaaauacgu uaauuuccug uaucucgcca gccauuacga gaagcugaaa     3960

ggcucccccg aagauaacga gcagaagcag cuuuucgugg aacaacacaa acauuaucug     4020

gacgagauca uugaacagau cucugaguuu agcaagcggg ucauucuggc ggaugcuaau     4080

cucgacaaag ugcuguccgc cuacaacaag caccgcgaua agccgaucag agagcaggcc     4140

gaaaacauua uccaccuuuu cacccugaca aaucucgggg cuccugccgc uuuuaaauau     4200

uucgacacca caauugaucg gaagcgcuac accagcacga aagagguucu ggacgccaca     4260

cucauccauc aaucuaucac cggccuguac gaaaccagaa uugaucuuuc ccagcuggga     4320

ggcgacaagc ggcccgcggc cacaaagaag gcugggcagg ccaagaaaaa gaaauaagga     4380

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     4440

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     4500

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaagaau u                                               4581


<210>  4779
<211>  5364
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4779
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaacuu caagauucug      240

ccgaucgcga uugaccucgg aguuaaaaau acaggcgugu uuagcgcuuu cuaccagaag      300

gggacauccc uggagcggcu ugauaacaag aauggaaaag uguaugaacu gucuaaggac      360

agcuacacgc uccugaugaa caaucgcacc gccagacggc aucaacgcag aggcaucgau      420

cggaaacagc uugucaagcg ccuguuuaaa cucauuugga cagagcagcu gaaccuggaa      480

ugggacaagg auacccagca agccaucucc uuccucuuua auagacgggg guucucuuuu      540

aucacagacg gauauagccc cgaguaccug aacauugugc cugaacaggu uaaggcuauc      600

cuuauggaua uuuucgacga uuauaauggc gaggacgauc uggacuccua ccucaaacug      660

gccaccgagc aggaaucuaa gaucagcgag auuuauaaca aacuuaugca gaagauccug      720

gaauuuaaac ucaugaagcu gugcacagau auuaaagacg acaagguguc caccaagacg      780

cugaaagaga ucaccagcua cgaguucgaa cuccuggcug auuaccuugc caauuauucu      840

gagucccuga agacacaaaa auuuagcuac accgacaagc agggcaaccu caaagaacug      900

ucuuauuacc accaugauaa guauaauauc caggaguucc uuaagcgcca cgcgacaauu      960

aacgacagaa uccuggauac ccuccugacg gacgaucugg acauuuggaa uuuuaacuuc     1020

gaaaaauucg auuuugacaa gaacgaggag aaacuccaga aucaagaaga uaaggaccau     1080

auccaggccc aucugcacca cuucgucuuu gcugugaaca aaauuaaguc cgagauggcc     1140

agcgggggac ggcaccgcuc ucaguacuuc caagaaauca caaauguucu ugacgagaac     1200

aaucaucagg aaggcuaucu gaagaacuuu ugugagaauc uccacaacaa aaaguacucc     1260

aaucugagcg ugaaaaaccu ugucaaucug aucgggaacc ucuccaaucu ggagcugaag     1320

ccccucagaa aauacuucaa cgauaagauu cacgcuaagg ccgaccauug ggaugaacag     1380

aaauuuaccg agacauauug ccacuggauc cugggagaau ggcggguggg cgugaaggac     1440

caggauaaaa aggacggggc gaaauacucu uauaaggauc uuuguaauga gcugaaacaa     1500

aaggucacca aggccggacu cguugacuuc cugcuggaac uugauccuug ccgcaccauu     1560

cccccuuacc ucgacaacaa caauagaaaa ccgcccaagu gucagagccu gauccugaac     1620

ccuaaauuuc ucgauaauca guaucccaac uggcagcaau accugcagga gcuuaagaaa     1680

cugcagucca uucagaauua ucucgacagc uucgagacag aucugaaggu gcugaagucu     1740

uccaaagacc aaccuuacuu uguggaauac aagagcucua accagcagau cgcuagcggc     1800

cagcgggacu auaaagaucu ugacgccaga auccuccaau ucauuuuuga ucgcgucaag     1860

gcuuccgacg agcugcugcu caaugaaauc uacuuccagg ccaaaaagcu gaagcagaaa     1920

gccucuagcg agcuugagaa gcuggaaucc agcaaaaagc ucgaugaggu gauugcuaac     1980

ucucaacugu cccagauccu gaaaagccag cauacgaaug gcauuuucga acaggggacc     2040

uuucuucacc ucguuugcaa guauuacaag caacggcaga gagcgcgcga cucucggcug     2100

uauaucaugc ccgaguacag auaugauaaa aagcugcaua aauacaacaa uacaggacgc     2160

uucgacgaug acaaccagcu ccugaccuac uguaaucaca agccgcggca gaaaagauau     2220

caacuucuga acgaucucgc cggcgugcug caggucuccc cuaauuuucu gaaggacaaa     2280

auugggagcg augacgaccu uuucaucucc aaguggcucg uggaacacau ccgcggauuu     2340

aagaaggcuu gcgaggauuc ucugaaaauu cagaaagaca accggggccu gcucaaccau     2400

aagaucaaua uugccagaaa cacaaaaggg aagugugaga aggaaaucuu caaucugauu     2460

ugcaaaaucg agggaagcga agauaagaaa ggcaacuaca agcacggccu ugccuaugag     2520

cuggggguuc uccuguuugg agaacccaau gaggcuucca aaccugaguu cgaccgcaag     2580

aucaagaaau uuaacucuau uuacagcuuc gcccagaucc aacagauugc guuugcugaa     2640

cggaagggca augccaacac cugugccgug ugcuccgcug auaaugccca uagaaugcag     2700

caaaucaaaa uuacggagcc cgucgaagac aacaaggaua aaaucauccu gagcgcuaag     2760

gcccagcggc uucccgcgau uccuacacgc aucguggacg gggccgucaa gaaaauggcu     2820

accauucugg ccaagaauau cguggaugac aacuggcaga auauuaaaca gguucucucu     2880

gcuaagcacc aacugcacau cccgaucauu accgagucca acgccuucga auuugagccc     2940

gcgcucgccg augugaaagg aaagagccug aaggacagac ggaaaaaggc ucuugagaga     3000

aucucuccug aaaauauuuu caaagacaag aacaaccgca ucaaagaguu cgccaagggc     3060

auuuccgcuu auagcggggc caaucugaca gauggcgacu uugauggagc caaagaggaa     3120

cucgaccaua ucauuccccg gucucacaag aaguacggca cccugaacga ugaggcuaau     3180

cugaucugcg ucacaagagg ggacaacaaa aauaagggaa accgcaucuu cugucuuaga     3240

gaucuggcgg acaauuauaa acucaagcag uuugaaacga ccgaugaccu ggagauugag     3300

aaaaagaucg ccgauacaau uugggacgcu aacaagaaag auuucaaguu uggcaauuac     3360

cggagcuuca ucaaccucac cccucaggaa cagaaagccu uucgccaugc ccuguuccuu     3420

gcugacgaaa aucccauuaa gcaagccgug aucagagcga ucaacaaucg gaaccgcaca     3480

uuuguuaaug ggacccagag auacuucgcu gaggugcugg ccaacaauau uuaucuccgg     3540

gccaaaaagg agaaccugaa cacggacaag aucuccuuug auuacuucgg cauuccgacc     3600

aucggaaaug ggcgcggcau ugcugaaauc agacagcugu augagaaagu cgacagcgau     3660

auccaggccu acgcgaaggg agacaaaccu caggcuucuu auucccaccu uauugaugcc     3720

augcuggccu ucugcaucgc ugccgacgaa caucggaacg auggcagcau ugggcucgag     3780

aucgacaaga auuacucccu guauccccuc gauaaaaaca caggagaagu guuuaccaag     3840

gacauuuucu cucaaaucaa gauuacagau aaugaguuua gcgacaaaaa gcuggugcgc     3900

aaaaaggcua ucgagggcuu caacacccac agacagauga cgcgggaugg gaucuacgcc     3960

gaaaauuacc uuccuauucu gauccacaaa gagcugaacg aaguuagaaa gggauauaca     4020

uggaaaaauu ccgaggaaau uaagaucuuu aagggcaaaa aguacgacau ucagcaacuc     4080

aacaaucugg ucuauugucu uaaauucgug gacaagccca ucucuaucga uauucagauc     4140

agcacccugg aggagcuccg caacauucug acaaccaaua acaucgcggc caccgcugaa     4200

uacuauuaca uuaaucucaa aacacagaag cugcaugagu auuacaucga aaacuacaac     4260

acggcccuug gguauaagaa auacuccaag gagauggagu uucugcgguc ucuggcuuau     4320

agaagcgaac gcgugaaaau caaguccauu gacgauguca aacaagugcu cgacaaggau     4380

agcaauuuca ucauuggcaa gaucacccug ccuuuuaaaa aggaguggca gcggcuuuac     4440

agagaauggc agaacacaac cauuaaagac gauuaugagu uccugaaguc uuucuucaau     4500

guuaaaucca ucacaaagcu ccacaagaaa gugcgcaagg acuuuagccu gcccauuucu     4560

accaacgaag gaaaauuccu cgucaagcgg aaaacguggg auaauaacuu caucuaccag     4620

auccugaaug acuccgauag cagagccgac ggcacaaagc cguuuauucc ugccuucgac     4680

aucucuaaaa acgagauugu ggaggcuauc auugauuccu uuaccagcaa gaauaucuuc     4740

uggcuuccca agaacaucga acugcaaaaa guugacaaua agaacauuuu ugcgaucgau     4800

accuccaaau gguucgaggu ggaaacaccc agcgaccugc gcgauauugg gaucgccacc     4860

auucaguaua agaucgacaa uaacucucgg ccuaaaguca gagugaagcu cgauuacguu     4920

aucgacgaug acagcaagau uaauuacuuu augaaccauu cccugcuuaa aucucgguau     4980

ccggauaagg ugcuggagau ccucaaacag agcacaauua ucgaauuuga guccucugga     5040

uuuaacaaga cgauuaaaga gaugcugggc augaagcucg cugggaucua caaugaaacc     5100

agcaacaaua agcgccccgc cgccaccaaa aaggcuggac aggccaaaaa gaaaaaguaa     5160

ggacuaguaa uaaggaaacu guaugaaugu cugugggcag gaagugaaga gauccuucug     5220

uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu uuugaaauaa uaaaucuggu     5280

agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5340

aaaaaaaaaa aaaaaaaaag aauu                                            5364


<210>  4780
<211>  3636
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4780
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagcg gaacuacauu      240

cugggacucg acaucggcau uaccagcguu ggguauggaa ucauugauua cgagacacgc      300

gacgugaucg augcgggcgu gagacuguuc aaagaagcua augucgagaa caaugaaggg      360

cggcgcucca agagaggagc ccggcgccuu aagagacggc gcagacaucg gauccagcgc      420

gugaaaaagc ugcucuuuga cuauaaccug cuuacggauc acucugagcu gagcggcauu      480

aauccguacg aagccagagu uaaaggccuc ucccaaaagc ugucugagga ggaguucagc      540

gcugcccugc uccaucuggc uaaacggcgc ggggugcaca acgucaauga aguggaagag      600

gacaccggaa acgagcuuuc cacaaaggaa cagaucucua gaaauagcaa ggcccuggag      660

gaaaaauaug uugcggagcu ccagcuggaa cggcuuaaga aagauggcga ggugagaggg      720

uccauuaacc gcuuuaagac cagcgacuac gucaaagagg ccaagcagcu gcucaaagug      780

caaaaggcuu aucaucagcu ggaucagucu uucaucgaca cauacauuga ucugcucgaa      840

acccggagaa cauacuauga gggacccggc gaaggguccc cuuuuggaug gaaggacauc      900

aaagaguggu acgaaaugcu gaugggccac ugcaccuauu uccccgagga gcuucgcagc      960

gugaaguacg ccuauaaugc ugaucuguac aacgcccuca augaccugaa caaucuuguc     1020

auuacgcggg acgaaaacga gaaacuggaa uauuacgaga aguuucagau caucgaaaac     1080

guuuucaaac aaaagaagaa accuacccuc aagcagauug cgaaagagau ccuggugaau     1140

gaggaagaua uuaagggcua cagagugaca ucuaccggga aacccgaguu uacaaaccug     1200

aaggucuauc acgacaucaa ggauauuacc gcccgcaaag aaaucaucga gaaugcugag     1260

cuccuggacc agauugccaa gauccuuacg auuuaccagu ccagcgaaga uauccaagag     1320

gaacugacaa accucaauuc ugagcugacc caggaagaga uugagcagau cuccaaccuu     1380

aaaggauaua caggcaccca uaaucugagc cucaaggcua ucaaccugau ucuggacgaa     1440

cucuggcaca ccaaugauaa ccaaaucgcc auuuucaauc ggcugaaacu ugugccuaag     1500

aagguugacc ugucccagca gaaagagauc ccgacaacgc ucguggauga cuuuauucug     1560

ucucccgucg ugaagcgcag cuucauccag uccauuaaag uuaucaacgc caucauuaag     1620

aaauacgggc ugccuaauga uaucauuauc gaacuugcuc gcgagaagaa cagcaaagac     1680

gcgcaaaaga ugauuaauga aaugcagaag cggaacagac agaccaacga gcgcaucgag     1740

gaaaucauuc ggacaaccgg aaaagagaau gccaaguauc ucaucgagaa aauuaagcug     1800

caugacaugc aggaaggcaa augucuguac ucucucgaag cuaucccccu ggaggaucuu     1860

cugaacaauc cuuuuaacua ugagguggac cacauuaucc ccagauccgu cagcuucgau     1920

aauucuuuca acaauaaggu gcucgucaag caagaagaga acagcaaaaa ggggaaucgg     1980

acaccguuuc aguaccuguc cucuagcgac uccaaaauca gcuacgaaac cuucaagaaa     2040

cacauucuga accuugccaa gggaaagggc cgcaucucua aaacgaagaa agaguaucuc     2100

cuggaggaaa gagauauuaa ucgguuuucc gugcagaagg acuucaucaa cagaaaucug     2160

guugauacac gcuacgccac ccggggccuc augaaccugc uuagaagcua uuuucgcgug     2220

aauaaccugg acgucaaagu gaagucuauu aacgggggau ucaccuccuu ucucagacgg     2280

aaguggaaau ucaagaaaga gcgcaauaag ggcuacaaac aucacgcuga agaugcccug     2340

aucaucgcga acgcugacuu uauuuucaag gaguggaaaa agcuggauaa ggccaaaaag     2400

guuauggaaa aucagauguu ugaggagaaa caagccgaaa gcaugccuga gaucgaaaca     2460

gagcaggaau auaaggagau uuucaucaca ccccaucaga uuaaacacau caaggacuuu     2520

aaggauuaca aauauuccca uagaguggac aagaaaccua accgggagcu uauuaaugac     2580

acccucuacu cuacgcgcaa ggaugacaaa gggaacaccc ugaucgucaa uaaccugaau     2640

ggacucuacg auaaggacaa cgauaagcug aagaaacuua ucaauaaaag ccccgaaaag     2700

cugcucaugu aucaccacga cccccagaca uaccaaaaac ugaagcugau uauggagcag     2760

uauggcgaug aaaagaaccc ucuuuacaaa uauuacgagg aaaccgggaa uuaucucaca     2820

aaguacucca aaaaggacaa cggcccggug aucaaaaaga uuaaguacua uggaaauaaa     2880

cugaacgcuc aucuggauau caccgacgau uaccccaacu cuagaaauaa ggugguuaaa     2940

cucagccuga agccuuaucg guucgacguc uaccuugaua acggcgugua uaaauucgug     3000

acggucaaga aucuggacgu gauuaaaaag gagaacuacu augagguuaa uuccaagugc     3060

uacgaagagg ccaaaaagcu caaaaagauc agcaaccagg cugaauuuau cgccucuuuc     3120

uacaauaacg accugauuaa aaucaauggg gagcuguauc gcgugauugg agucaacaau     3180

gaucuucuga acagaaucga ggugaauaug auugacauca ccuaccggga auaucucgag     3240

aacaugaaug auaagagacc cccuagaauc auuaagacaa ucgcguccaa aacccaaagc     3300

auuaagaaau acucuacaga cauccugggc aaccucuaug aaguuaaguc caaaaagcac     3360

ccccagauua ucaagaaagg gaagcggccg gccgcuacca aaaaggccgg acaggcuaaa     3420

aagaagaaau aaggacuagu aauaaggaaa cuguaugaau gucugugggc aggaagugaa     3480

gagauccuuc uguaaagguu uuuggaauua ugucugcuga auaauaaacu uuuuugaaau     3540

aauaaaucug guagaaaaau gagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3636


<210>  4781
<211>  4704
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4781
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcccuguu caacaagugc      240

auuaucauua gcaucaaucu cgacuuuucc aacaaagaga aguguaugac caagccguac      300

ucuauuggac uggauaucgg cacaaauagc guuggguggg cggugaucac ggacaacuau      360

aaagugcccu ccaagaaaau gaagguccuu ggaaauaccu cuaaaaagua cauuaagaaa      420

aaccugcucg gcgugcugcu uuucgauagc gggaucacag cugaaggacg gcgccugaag      480

agaaccgccc ggcgcagaua uacacggcgc agaaaucgga uucucuaccu gcaggagauc      540

uuuuccaccg aaauggccac acuggacgau gcuuucuuuc aacgccucga cgauucuuuc      600

cugguuccug acgauaaaag agacagcaag uaucccauuu uuggcaaccu uguggaggaa      660

aaagucuacc augaugaguu cccuaccauc uaucaucugc ggaaguaccu cgccgacucc      720

acgaaaaagg cugaucugcg ccuuguguac cuggcccucg cgcacaugau uaaguauaga      780

ggccacuuuc ugaucgaggg ggaguucaau agcaaaaaca augacaucca gaagaacuuu      840

caggacuucc uggauaccua caaugccauu uuugaaucug accucucccu ggaaaacagc      900

aaacagcuug aggagaucgu uaaggauaaa auuucuaagc uggaaaagaa agaccggauc      960

cucaagcugu uccccggaga gaaaaauucc ggcauuuuca gcgaauuucu uaagcugauc     1020

guggggaacc aagcugauuu ccgcaaaugc uuuaaccucg acgagaaggc cucucugcau     1080

uucuccaagg aaagcuauga ugaggaccug gagacacucc ugggauacau cggcgaugac     1140

uauuccgaug ucuuucuuaa agcuaagaaa cuguacgacg ccauucuccu gucuggguuc     1200

cuuaccguga cagacaauga aaccgaggcg ccucugagcu ccgccaugau caagagauau     1260

aacgaacaca aagaggaucu cgcucugcug aaggaauaca uucggaauau cagccucaag     1320

acguacaacg agguguuuaa agacgauaca aagaauggau augccggcua cauugacggc     1380

aaaaccaacc aggaggauuu cuaugucuac cugaagaauc uucuggcuga auuugagggg     1440

gccgacuauu uccucgaaaa gaucgauaga gaggacuuuc ugcgcaagca gcggacauuc     1500

gauaacggau cuaucccgua ccagauucau cugcaagaaa ugagagccau ccuugacaaa     1560

caggcuaagu uuuaucccuu ccucgcgaag aauaaagagc gcauugagaa gauccugacc     1620

uuccggauuc cuuacuacgu uggcccccug gccagaggga acuccgauuu ugcuuggagc     1680

auccgcaaac ggaaugaaaa gauuaccccu uggaacuucg aggacgugau cgauaaagaa     1740

ucuagcgccg aggccuuuau caauagaaug acauccuucg accucuaucu gcccgaggaa     1800

aaggugcuuc cgaagcacuc ucugcucuac gagacguuua acgucuauaa ugaacugacc     1860

aaagugcgcu ucauugcuga gagcaugcgg gacuaccagu uucuggauuc caagcagaaa     1920

aaggacaucg uuagacuuua uuucaaagau aagcgcaagg ugacagacaa agauauuauc     1980

gaauaccucc acgccauuua uggauacgac ggcaucgagc ugaaggggau cgagaaacaa     2040

uuuaacagcu cucuguccac cuaccaugau cuccugaaca uuaucaauga caaggaguuc     2100

cuugaugaca gcucuaacga agcgauuauc gaagagauua uccacacacu gaccaucuuu     2160

gaagaucggg agaugauuaa acagagacuc uccaaguucg agaauaucuu ugacaagagc     2220

guccugaaaa agcugucccg gcgccacuau acgggauggg gcaaacuuuc ugcuaagcuc     2280

auuaacggca ucagagauga aaagagcggg aauacaauuc uggacuaccu gaucgacgau     2340

ggaaucucca accggaauuu caugcagcuc auucaugacg augcccuguc uuucaagaaa     2400

aagauccaaa aggcccagau uaucggcgac gaggauaaag ggaacauuaa ggaagugguu     2460

aaaagccuuc cuggaucccc cgcuaucaag aaaggcauuc ugcagagcau caagaucgug     2520

gacgagcucg ucaaggugau gggcgggaga aaaccugaau cuauugucgu ggagauggcc     2580

cgcgagaauc aguauaccaa ccaaggaaag uccaauagcc agcagcggcu gaaaagacug     2640

gaaaagucuc uuaaagagcu cggcuccaag auccugaagg aaaacauucc cgcuaaacug     2700

agcaagaucg auaauaacgc ccuccagaau gaccgccugu accuuuauua ccugcaaaac     2760

gggaaagaua uguauaccgg agacgaucuc gacauugaca gacugucuaa cuacgauauc     2820

gaccacauca uuccccaggc guuucugaag gauaauagca ucgacaacaa aguucuugug     2880

uccagcgccu cuaaucgggg caaguccgau gacuucccua gccuggaggu cgugaagaaa     2940

cgcaagacau uuugguacca gcuccugaaa uccaagcuca uuucucagag aaaauucgau     3000

aaccugacca aggcugagcg cgggggacuu cugccggaag acaaggccgg cuuuauccaa     3060

cggcagcucg uugagacaag acagauuacg aaacaugugg cucggcugcu ggaugaaaag     3120

uucaauaaca aaaaggacga gaauaaccgc gccgucagaa ccgugaaaau caucacacuu     3180

aagagcaccc ugguguccca guuucggaaa gauuucgaac ucuauaaggu ucgcgagauu     3240

aaugacuuuc accaugcgca cgaugccuac cugaacgcug ucaucgccuc ugcucuccug     3300

aagaaauauc ccaaacuuga gccugaguuc guguacgggg acuaucccaa auacaauagc     3360

uuuagagaac ggaaguccgc cacagaaaaa guguauuucu acucuaacau uaugaauauc     3420

uucaaaaaga gcauuucccu ggccgacggc agagucaucg agcgcccucu cauugaagug     3480

aacgaggaga ccggagaaag cguuuggaau aaagagucug aucuggcuac ggugcggaga     3540

guccuguccu acccccaagu gaacguugug aagaaagucg aggaacagaa ccacggccuu     3600

gaccgcggga agccgaaagg acuguuuaau gcgaaccuca gcucuaagcc uaagcccaau     3660

uccaacgaaa aucugguggg cgccaaagag uaucucgauc cuaagaaaua cgggggauau     3720

gcuggcauca gcaacucuuu cgccguucug gugaagggga ccaucgagaa aggcgccaag     3780

aagaaaauua caaauguccu ugaauuucag ggaaucucca uucuggaccg gaucaacuac     3840

agaaaggaua aacugaauuu ccuccuggag aagggcuaua aagacauuga acuuaucauc     3900

gagcugccca aguacagccu cuuugagcug uccgauggga gccgccggau gcucgcuucu     3960

auucugagca ccaacaauaa aagaggagaa auccauaagg gcaaccaaau uuuccuuucc     4020

cagaaguuug ugaaacugcu guaucacgcc aagcgcaucu cuaauacaau uaacgagaau     4080

caucggaaau acguggaaaa ccacaagaaa gaguucgagg aacucuuuua cuauauccug     4140

gaguucaaug aaaacuacgu cggggcgaag aagaacggaa aacuucugaa uagcgcuuuu     4200

caguccuggc agaaccacuc uaucgacgag cucuguagcu ccuucauugg cccuaccggg     4260

ucugaaagaa agggccuguu ugagcucacg agccggggau ccgccgccga uuucgaguuc     4320

cugggcguua aaaucccccg cuauagagac uacacaccga gcucucuucu gaaggaugcu     4380

acccugauuc aucaauccgu gacagggcuc uaugaaaccc ggaucgaccu ggccaaacuu     4440

ggagagggca agcgcccugc ggcuaccaag aaagccgggc aggccaagaa aaagaaauaa     4500

ggacuaguaa uaaggaaacu guaugaaugu cugugggcag gaagugaaga gauccuucug     4560

uaaagguuuu uggaauuaug ucugcugaau aauaaacuuu uuugaaauaa uaaaucuggu     4620

agaaaaauga gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4680

aaaaaaaaaa aaaaaaaaag aauu                                            4704


<210>  4782
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4782
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccuggua cgcgagccug      240

auguccgcuc aucaccuccg gguuggaauu gacgugggca cccauucugu ggggcuggcc      300

acacuucgcg ucgaugacca cggaacgccg aucgagcugc ucagcgcccu gucccauauu      360

cacgauucug gcguggggaa ggaagggaaa aaggaccacg auaccagaaa gaaacuuagc      420

ggcaucgcuc ggcgcgccag acggcugcuc caucaccgca gaacacagcu gcaacagcug      480

gacgagguuc uccgggaucu gggcuucccc auuccuaccc ccggggaauu ucuugaccug      540

aacgagcaga cagauccuua ucgcgugugg agaguccggg cucgccucgu ggaagagaag      600

cugcccgagg aacuuagagg accugccauc uccauggcgg uucggcauau cgcccgccac      660

agaggcuggc ggaauccgua cucuaaagug gagagccugc ucucccccgc ugaagagagc      720

ccuuucauga aggcccugag agagcgcauu cuggcuacca caggggaagu ccucgacgau      780

ggaaucaccc ccggccaggc cauggcgcaa guggcccuga cgcacaacau uucuaugcgg      840

gggccugagg gaauccuugg caaacugcau caguccgaca augcuaacga aauuagaaag      900

aucugcgccc gccagggcgu gagccccgau gucuguaagc agcuccugcg ggcuguuuuu      960

aaagccgacu cuccgagagg guccgccgug agccgcgugg cuccugaccc ccuuccugga     1020

caaggcucuu uccggagagc gcccaagugc gaucccgagu uucagcgcuu ccggauuauc     1080

uccaucgucg ccaaucugag aauuagcgaa accaaagggg agaaccgccc ucucacagcu     1140

gacgagcgga gacacguggu uaccuuucug acagaagauu cccaggccga ccugaccugg     1200

guggaugucg ccgagaagcu cggagugcau cggcgcgacc ugagaggcac ggcuguucac     1260

acagaugacg gggaacgguc ugccgcgaga ccgcccaucg augcuaccga ccgcauuaug     1320

cggcagacaa aaaucagcuc ccuuaagacc uggugggagg aagccgauag cgagcaaaga     1380

ggagccauga uucgcuaucu guacgaggac ccuaccgacu cugaaugugc ugagaucauc     1440

gccgaacucc ccgaggaaga ucaggcuaaa cuggacuccc uucaucugcc ugccggcaga     1500

gcggccuaua gccgggaguc ucucacagcu cugagcgauc acaugcuggc cacgaccgac     1560

gaucuccacg aggcucgcaa gagacuguuc ggcguggacg auuccugggc gcccccggcc     1620

gaagccauua augcuccugu cgggaacccc ucuguggacc ggacacuuaa gaucgucgga     1680

cgcuaccuga gcgccgugga guccaugugg ggcaccccug aaguuauuca uguggagcac     1740

gucagagaug gguuuacaag cgagcggaug gcugacgaac gcgauaaagc caauagacgg     1800

cgcuauaacg acaaucagga ggccaugaag aaaauccaaa gagauuacgg aaaggaaggc     1860

uacauuucuc ggggagacau cgugagacuc gacgcucugg agcugcaggg cugcgcgugu     1920

cuuuauugcg gaaccacgau cggcuaccau acaugucagc ucgaucacau uguuccccag     1980

gccgggccug gauccaacaa ucgccggggc aaccuggugg cugucugcga aagauguaau     2040

cgcagcaaau cuaacacccc cuucgccgug ugggcccaaa agugcgggau cccgcacgug     2100

ggaguuaagg aggcuauugg ccgggucaga ggguggcgca aacagacccc uaacacaucc     2160

agcgaggacc ugacccggcu caagaaagaa gugaucgcca gacugcgccg gacacaggag     2220

gaucccgaaa uugacgagag auccauggaa ucuguggcgu ggauggcuaa ugagcuucau     2280

caccggaucg ccgccgcuua ucccgagacg accgucaugg uguaccgcgg cagcauuaca     2340

gccgcggcua gaaaggccgc cggaaucgau ucccggauca accugauugg cgaaaaaggg     2400

cgcaaggaca gaaucgaucg gagacaucac gcuguugacg ccucuguggu cgcucucaug     2460

gaggccagcg uggcgaagac ccuggccgaa cgcuccagcc ugcggggaga gcagagacuu     2520

acaggcaaag aacaaaccug gaagcaguau acggggucua ccguuggagc ucgcgagcau     2580

uuugagaugu ggcggggcca caugcuccac cugacagaac uguucaauga gagacucgcc     2640

gaagauaaag uguacgucac ccagaacauu cgccugcggc uuuccgacgg gaaugcucau     2700

acagugaacc cuagcaagcu gguuucucac agacucggcg auggacugac cgugcagcaa     2760

aucgaccgcg ccuguacgcc ggcccugugg ugcgcucuua caagagagaa agauuuugac     2820

gagaagaaug gccuccccgc gcgggaagac cgcgccauua gaguccaugg gcacgagauc     2880

aaguccagcg auuauaucca gguguucucu aaacggaaga aaaccgacag cgaucgcgac     2940

gaaacaccuu ucggagcuau ugccgugaga ggcggguuug ucgagaucgg acccuccauu     3000

caucacgccc ggaucuacag aguugaaggc aagaaaccug uguacgcuau gcugcgcgug     3060

uucacccacg aucugcucag ccagcggcau ggggaccugu uuucugccgu cauucccccu     3120

caguccauca gcaugagaug cgcggagccc aaacuucgca aagcuaucac cacaggcaac     3180

gccacguauc ugggaugggu gguugugggc gaugagcucg aaauuaaugu cgacuccuuc     3240

accaaguacg ccaucgggcg guuucuggag gauuucccga acacaaccag auggcggauu     3300

uguggauaug acacaaauuc uaagcugacc cuuaaaccua ucgugcucgc ugccgaaggc     3360

cuggagaacc ccagcuccgc gguuaaugaa auuguggagc ugaaggggug gcgcgucgcu     3420

aucaacgugc ucacgaaagu ucacccuaca guggucagac gggaugcccu gggacgcccc     3480

agauacucua gccgguccaa ucuuccgacc ucuuggacca ucgagaagcg cccugccgcu     3540

acaaagaagg ccggccaagc uaagaaaaag aaauaaggac uaguaauaag gaaacuguau     3600

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     3660

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3780


<210>  4783
<211>  4641
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4783
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaccaa gccguacagc      240

auuggacugg acaucggcac aaacuccguu gggugggcgg ugacgaccga uaauuauaaa      300

gugcccucua agaagaugaa aguccucgga aacacaagca agaaauacau uaagaaaaau      360

cugcuuggcg ugcuccuguu cgacuccggg aucaccgcug agggacggcg ccugaagaga      420

acagcccggc gcagauauac ccggcgcaga aaccggauuc uuuaccugca ggaaaucuuu      480

ucuacagaga uggccacccu cgaugacgcu uucuuucaac gccuggauga cagcuuccug      540

guuccugaug acaagagaga uuccaaauau cccaucuuug gcaaucucgu ggaagagaag      600

gccuaccaug acgaguuccc uacgauuuau caucugcgga aauaccuugc ugauucuacc      660

aagaaagccg accugcgccu cgucuaccug gcgcuugccc acaugaucaa guauagaggc      720

cacuuucuga uugaggggga guucaacagc aaaaauaacg auauccagaa gaauuuucag      780

gacuuccucg acacauacaa cgcuauuuuu gaauccgauc ugagccugga aaauucuaag      840

cagcucgaag agaucgugaa agacaagauu uccaaacugg agaagaaaga ucggauccuu      900

aagcuguucc ccggagaaaa gaacagcggc aucuuuucug aguuccucaa acugauuguu      960

gggaaucaag ccgacuuccg caagugcuuu aaccuugaug aaaaggcuuc ccugcauuuc     1020

agcaaagagu cuuaugacga agaucucgag acccugcugg gauacaucgg cgacgauuau     1080

uccgacgugu uucucaaagc caagaagcug uacgaugcga uucuucugag cggguuccuc     1140

acagucaccg acaaugagac ggaagccccu cuguccucug cuaugaucaa aagauauaac     1200

gagcacaagg aagaccuugc ccugcucaaa gaguacauuc ggaacaucag ccugaagaca     1260

uacaaugaag uguuuaaaga ugacaccaag aacggauaug cuggcuacau cgauggcaag     1320

acaaaucagg aggacuucua uguguaccug aaaaagcucc uggccgaguu ugaaggggcc     1380

gauuauuucc uugagaaaau ugacagagaa gauuuucugc gcaagcagcg gaccuucgac     1440

aacggaucca ucccguacca gauucaccuc caagagauga gagcuauccu ggauaaacag     1500

gcgaaguuuu aucccuuccu ggccaaaaau aaggaacgca uugagaagau ccuuaccuuu     1560

cggaucccuu acuacgucgg cccccucgcu agagggaaca gcgacuucgc cuggucuauu     1620

cgcaaacgga augagaagau cacaccuugg aacuucgaag auguuauuga caaagagucc     1680

agcgccgagg cuuuuaucaa uagaaugacg ucuuucgauc uguaucugcc cgaagagaag     1740

gugcucccga aacauagccu gcuuuacgaa accuuuaacg uguauaauga gcugacaaag     1800

guccgcuuca uugccgaauc caugcgggac uaccaguuuc ucgacucuaa gcagaaaaag     1860

gauaucguga gacuguauuu caaagacaag cgcaaaguua ccgauaagga cauuaucgag     1920

uaccugcacg cgaucuaugg auacgauggc auugaacuua aggggaucga gaaacaauuu     1980

aacagcuccc ucagcacaua ccaugaccug cugaauauua ucaacgauaa ggaguuccuc     2040

gacgauucuu ccaaugaagc uauuaucgag gaaaucauuc acacccugac gaucuuugag     2100

gaccgggaaa ugauuaaaca gagacuuagc aaguucgaga acaucuuuga uaaaucugug     2160

cugaagaagc ucucccggcg ccacuauaca ggauggggca aacugagcgc caagcugauu     2220

aacggcauca gagacgagaa auccgggaau accauccuug auuaccucau ugacgacgga     2280

aucucuaacc ggaauuucau gcagcugauu caugaugacg cccugagcuu uaagaaaaag     2340

auccaaaaag cucagauuau cggcgaugaa gacaagggga acaucaagga ggucgugaaa     2400

ucccucccug gaucucccgc cauuaagaaa ggcauccugc agagcauuaa gaucguugau     2460

gaacuuguga aagucauggg cgggagaaag ccugagucca uuguggucga aauggcucgc     2520

gagaaucagu auaccaacca aggaaagagc aauucucagc agcggcugaa aagacucgag     2580

aagucccuga aagaacuggg cagcaagauc cuuaaagaga acauucccgc caagcucucu     2640

aagaucgaca auaacgcgcu gcagaaugau cgccuguacc ucuauuaccu gcaaaacggg     2700

aaagacaugu auacaggaga ugaccuugau aucgacagac uguccaauua cgacauugau     2760

cacaucauuc cccaggccuu ccucaaggac aacagcaucg auaauaaagu gcugguuucu     2820

agcgcuucca accggggcaa gagcgacgau gugccuucuc uggaagucgu gaaaaagcgc     2880

aagaccuucu gguaccagcu ucugaaaucc aagcucauua gccagagaaa auuugacaac     2940

cugacaaagg ccgagcgcgg gggacucucc ccggaggaua aagcuggcuu cauccaacgg     3000

cagcugguug aaacgagaca gaucaccaag cauguggccc ggcuucugga cgagaaguuu     3060

aauaacaaaa aggaugaaaa uaaccgcgcg gucagaacag ugaaaauuau cacccucaag     3120

ucuacacugg ugagccaguu ccggaaagac uuugagcugu auaagguucg cgaaauuaau     3180

gauuuccacc augcccacga cgcuuaccuu aacgccgucg uggcuuccgc ccugcucaaa     3240

aaguauccca agcuggagcc ugaguuugug uacggggauu aucccaaaua caauucuuuc     3300

agagaaagaa agagcgccac cgagaaaguc uauuuuuacu ccaacaucau gaauauuuuc     3360

aagaaaucua ucagccucgc ugacggccgg gugaucgaac gcccucugau ugagguuaac     3420

gaagagacgg gagaguccgu guggaauaag gaaagcgacc uugcgaccgu ccggagagug     3480

cugucuuacc cccaaguuaa cguggucaag aaaguggagg aacagaauca cggccucgau     3540

cgcgggaagc cgaaaggacu guuuaacgcc aaucugucca gcaagccuaa acccaacucu     3600

aacgagaauc uuguuggcgc uaaggaauau cuggacccua agaaauacgg gggauaugcc     3660

ggcaucucca acagcuucac agugcucguc aaggggacca uugagaaagg cgccaagaaa     3720

aagaucacaa augugcugga guuccaggga auuucuaucc ucgaucggau uaacuacaga     3780

aaggacaaac ugaauuuucu ucuggaaaag ggcuauaaag auaucgagcu gaucauugaa     3840

cuccccaagu acucccuguu cgagcuuagc gacggguccc gccggaugcu ggcuagcauc     3900

cucucuacca acaauaaaag aggagagauu cauaagggca accaaaucuu ucugagccag     3960

aaauucguga agcuccugua ucacgccaag cgcauuucca auacgaucaa cgaaaaucau     4020

cggaaauacg ucgagaacca caagaaagaa uuugaggaac uuuucuacua uauccuggag     4080

uuuaaugaga acuacguugg ggcgaagaaa aauggaaagc ugcucaacuc ugcuuuccag     4140

agcuggcaga aucacuccau ugaugaacug uguucuagcu uuaucggccc uacagggucc     4200

gagagaaagg gccuuuucga acugaccucu cggggaagcg ccgccgacuu ugaguuccuc     4260

ggcgugaaaa uuccccgcua uagagauuac acaccgucca gccugcucaa ggacgcuacc     4320

cugauccauc aaucugugac cgggcuuuau gaaacacgga uugaucuggc caaacuggga     4380

gagggcaagc gcccugcggc uacgaaaaag gccgggcagg ccaagaaaaa gaaauaagga     4440

cuaguaauaa ggaaacugua ugaaugucug ugggcaggaa gugaagagau ccuucuguaa     4500

agguuuuugg aauuaugucu gcugaauaau aaacuuuuuu gaaauaauaa aucugguaga     4560

aaaaugagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaaagaau u                                               4641


<210>  4784
<211>  3723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4784
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgcggc uuucaagccg      240

aacagcauua auuacauccu gggacucgac auuggcaucg ccuccguugg gugggccaug      300

guggagauug augaagagga aaaccccauc cggcugaucg accuuggagu gcgcgucuuu      360

gagagagcug aagugccuaa aaccggcgau ucucuggcca uggcucggcg ccucgccaga      420

agcguucggc gccugacaag acggcgcgcg cauagacuuc ugcggacgcg cagacuccug      480

aagcgggagg gggugcugca ggccgcuaau uucgacgaga acggacucau uaagucccug      540

cccaauaccc cuuggcaacu ucgcgccgcu gcccuggaua gaaaacucac accccuggaa      600

uggucugcgg uccuucugca ccucaucaag caucggggcu aucugagcca gcgcaaaaac      660

gagggcgaaa ccgccgacaa ggagcugggg gcucuccuga aaggaguggc cggcaaugcu      720

cacgcccuuc agacagggga uuuuagaacc ccugccgagc uggcucucaa caaguucgaa      780

aaggaguccg gacauauucg gaaucagaga ucugacuaca gccacacauu uucccgcaaa      840

gaucugcaag cggaacuuau ccuccuguuc gagaagcaga aagaauuugg caacccgcac      900

guuagcgggg gacugaagga gggcauugag acccugcuca ugacgcagcg gcccgcccug      960

ucuggcgacg cugugcagaa aaugcuuggg cauugcaccu ucgaaccugc cgagcccaag     1020

gccgcuaaaa auacauauac cgccgaaaga uuuaucuggc ugacaaagcu caacaaucug     1080

cgcauucuug agcaaggauc cgaacggccu cugaccgaua cggagagagc gacacucaug     1140

gacgagcccu accgcaagag caaacugacc uaugcucagg cccggaagcu gcucggccug     1200

gaagauacag ccuucuuuaa agggcuuaga uacggaaagg acaacgcuga ggccucuacc     1260

cugauggaaa ugaaggcuua ucacgccauc ucccgcgcgc ucgagaagga aggccugaag     1320

gacaaaaaga gcccgcugaa ucuuucuccu gagcuccagg augagaucgg gaccgccuuc     1380

ucccuguuua aaacagacga agauauuacg ggacggcuga aggacagaau ccagcccgag     1440

auucucgaag cucugcuuaa acauaucagc uucgauaagu ucguccaaau uucccugaag     1500

gcccuccggc gcaucgugcc ucugauggag cagggcaaaa gauacgacga ggcuugugcc     1560

gaaaucuacg gcgaucacua ugggaagaaa aacaccgagg aaaagauuua ccugccuccc     1620

auccccgcgg acgagauucg gaacccggug guccuuagag cccucucuca ggcucgcaaa     1680

guuaucaaug gaguggugcg gagauauggc agccccgccc gcauucacau cgaaacagcu     1740

agagaggucg ggaaguccuu uaaggaucgg aaagagaucg aaaagcgcca agaggaaaac     1800

agaaaagacc gggagaaggc cgccgcuaaa uuccgcgaau acuuuccuaa uuucguggga     1860

gagcccaaga gcaaagauau ucugaagcug agacucuaug agcagcagca uggcaagugc     1920

cuguacucug ggaaagaaau caaccuuggc cggcugaaug agaagggaua uguugaaauu     1980

gaccacgcgc ucccuuuuuc ccgcaccugg gacgauagcu ucaacaauaa agugcugguc     2040

cugggcucug agaaccagaa uaaggggaac caaacacccu acgaauacuu uaauggaaaa     2100

gacaacagca gagaguggca ggaguucaag gcccgggugg aaaccucccg cuuuccgaga     2160

ucuaagaaac agcggauccu ucuccagaag uucgaugagg acggcuuuaa agaaagaaau     2220

cugaacgaua cgcgcuaugu uaaucgguuc cugugucaau uuguggcuga cagaaugcgc     2280

cucacaggga agggcaaaaa gcgggucuuc gccagcaacg gacagauuac caaccugcuu     2340

agaggguucu ggggccugcg caaggugcgg gccgagaaug auagacauca cgcucucgac     2400

gccgucgugg uugcgugcuc caccguggcu augcagcaga aaaucacacg cuuuguccgg     2460

uacaaggaga ugaacgccuu cgauggaaaa accauugaca aggaaacagg cgaggugcug     2520

caucagaaaa cgcacuuucc ucagcccugg gaguucuuug cccaggaagu uaugaucaga     2580

guguucggga agccugaugg aaagcccgaa uuugaggagg cugacacccu ggaaaaacuu     2640

cggacacucc uggccgagaa gcugagcucu cgcccugaag cgguccacga guaugugaca     2700

ccccucuucg uguccagagc uccgaaucgg aaaaugagcg gccaggggca uauggaaacc     2760

guuaagucug ccaaacgccu ggaugaggga gucuccgugc uuagagugcc ucugacccaa     2820

cucaagcuga aagaccugga gaagaugguc aaccgggaaa gagagcccaa acuuuacgaa     2880

gcccugaaag cucgccucga ggcccacaag gacgaucccg cuaaagccuu ugcggaaccu     2940

uucuauaagu acgacaaagc cggcaaucgg acgcaacagg ugaaggcugu uagaguggag     3000

caaguccaga agaccggggu guggguucgc aaccauaaug gcaucgccga uaacgcuaca     3060

auggugcggg ucgacguguu ugagaaagga gauaaguauu accugguucc gauuuacagc     3120

uggcaagugg ccaaaggcau ccuccccgac agagccgucg ugcaggggaa ggaugaagag     3180

gacuggcagc ugauugauga cuccuucaau uuuaaauucu cucuucaccc uaacgaucug     3240

guggaaguca ucaccaagaa ggcucgcaug uucggauauu uugcgagcug ucaccggggc     3300

acagggaaua uuaacaucag aauccaugac cucgaucaca aaauuggaaa gaauggcauc     3360

cuggagggga uuggcguuaa aaccgcccug uccuuccaga aguaccagau cgacgagcuu     3420

ggaaaagaaa uucgccccug cagacugaag aagcggccuc ccgugcgcaa aagaccugcu     3480

gccacgaaga aagccggcca ggcuaagaaa aagaaguaag gacuaguaau aaggaaacug     3540

uaugaauguc ugugggcagg aagugaagag auccuucugu aaagguuuuu ggaauuaugu     3600

cugcugaaua auaaacuuuu uugaaauaau aaaucuggua gaaaaaugag aucuaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     3720

auu                                                                   3723


<210>  4785
<211>  4479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4785
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagaa accguacacc      240

auuggacugg acaucggcac aaacagcguu gggugggcgg ugcucacgga ucaguaugac      300

cuggugaagc ggaagaugaa aauugcugga gauuccgaga agaaacaaau caagaaaaau      360

uucuggggcg uccgccuuuu ugacgaaggg cagaccgccg ccgauagacg gauggcucgc      420

acagccagac ggcgcauuga gagacggcgc aacagaaucu cuuaccugca gggaaucuuc      480

gcugaagaga ugagcaagac cgacgccaau uucuucugcc ggcucuccga uucuuuuuau      540

guggacaacg aaaagcgcaa uagcagacau cccuucuuug cgacaauuga ggaggaaguu      600

gaguaccaca aaaacuaucc uaccaucuac caucugcggg aggaacuugu caauuccucu      660

gagaaggccg aucugcgccu cgucuaucug gcucuggccc acauuaucaa auacagaggc      720

aacuuccuca uugaaggcgc ucuggacaca cagaauacca gcguggaugg gaucuacaag      780

caauuuauuc agacguauaa ccagguuuuc gccuccggaa ucgaggacgg cagccuuaaa      840

aagcuggaag auaauaaaga cguggcgaag auccucgucg agaaggugac ccggaaagaa      900

aagcuggagc gcauucuuaa acuguacccc ggggagaagu cugccggaau guuugcucag      960

uucaucuccc ucauuguggg cagcaaaggg aacuuucaaa agccuuucga ccugaucgaa     1020

aagucugaua uugagugugc caaagacucc uaugaagagg aucuggaaag ccuccuggcu     1080

cuuaucggag acgaguacgc cgagcuguuc gucgccgcua agaaugcgua uucugccguu     1140

gugcucucca gcaucauuac aguggcugaa accgagacaa acgccaaacu guccgccucu     1200

augaucgaaa gauuugauac ccaugaggaa gaccuuggcg agcugaaggc uuucauuaaa     1260

cuccaucugc ccaagcacua cgaggaaauc uuuagcaaua cggagaagca cggcuaugcc     1320

ggguacauug auggaaaaac aaagcaggcg gacuucuaca aauauaugaa gaugacccug     1380

gaaaacaucg agggcgcuga uuacuuuauc gccaaaauug agaaggaaaa cuuccuccgg     1440

aagcagagaa cauuugacaa uggggccauc ccucaccagc ugcaucuuga ggaacuggag     1500

gcuauucucc accaacaggc caaauauuac ccguuccuga aggaaaacua ugauaaaauc     1560

aagucccuug ucaccuuucg cauucccuac uucgugggac cucuggcuaa uggccagagc     1620

gaguuugccu ggcucacccg gaaagcggac ggggagauca gacccuggaa cauugaagag     1680

aagguugacu ucggaaaauc ugccguggau uuuaucgaaa agaugacaaa uaaggacacg     1740

uaucugccua aagagaacgu ccugcccaag cacucccucu gcuaccaaaa auaccuggug     1800

uauaaugaac uuaccaaggu ucgcuacauc aacgaucagg gcaaaacaag cuauuucucu     1860

ggccaggaga aggagcagau uuucaaugac cuguuuaagc aaaaacggaa ggugaaaaag     1920

aaagaucucg aacuguuccu gagaaacaug agccaugucg aguccccgac caucgaaggg     1980

cuugaggacu cuuuuaauag cuccuacagc acauaucacg aucuccugaa ggugggaauu     2040

aagcaggaaa uccuggacaa cccugucaau accgagaugc ucgagaacau ugugaaaauc     2100

cugacgguuu ucgaagauaa gcgcaugauc aaagagcagc uucagcaauu uucugacgug     2160

cuggauggcg ucgugcucaa gaaacuggaa cggagacauu acacagggug gggacgccug     2220

uccgcuaagc uccucauggg cauucgggac aagcagagcc accugaccau ccuggauuau     2280

cucaugaaug acgacgggcu gaacagaaac cuuaugcagc ugauuaauga uucuaaccuc     2340

uccuucaaaa gcaucauuga gaaggagcag guuaccacag ccgacaaaga uauccaaucc     2400

aucguggcug accuggccgg cucucccgcg auuaagaaag gaauccugca gagccuuaag     2460

auugucgaug aacucguguc cgugaugggc uacccucccc agaccaucgu ugucgagaug     2520

gcccgggaaa aucagacaac ggggaaagga aagaacaauu cucgccccag auacaagagc     2580

cuggagaaag cuauuaagga auuuggcucc caaauccuga aagagcaucc uaccgacaac     2640

caggagcucc ggaauaacag acuguaucuu uacuaucugc agaaugggaa ggauauguac     2700

acaggacaag accucgauau ccacaaccug agcaauuaug acauugauca caucgugccg     2760

cagucuuuca uuaccgacaa cuccaucgau aaucuggugc uuacaagcuc ugccggcaac     2820

cgcgaaaaag gggacgacgu gcccccucuc gagauugugc ggaagagaaa gguguuuugg     2880

gaaaaacugu accagggcaa ucugaugucc aagcgcaaau ucgauuaucu caccaaggcu     2940

gagagaggag gccugacgga agccgacaaa gcccgguuua uccaucgcca gcuuguugag     3000

accagacaaa uuacaaagaa cgucgcuaac auccugcacc agcgguucaa uuacgagaag     3060

gaugaccaug ggaacaccau gaaacaggug cgcaucguua cacucaagag cgcgcuggug     3120

ucucaguuua gaaaacaauu ccagcuguac aagguccggg augugaauga cuaucaccac     3180

gcccaugaug cuuaccuuaa cggaguugug gccaauaccc ugcucaaagu cuauccccag     3240

cuggaaccug aguucgugua cggcgacuau caccaguuug auugguucaa ggccaacaag     3300

gcuacggcca aaaagcaauu uuacacaaau auuaugcuuu ucuuugcgca gaaagaccgc     3360

aucauugaug aaaacgggga gauccugugg gacaagaaau aucuugacac cgugaagaag     3420

gucaugagcu acagacagau gaauauuguu aaaaagacag aaauccaaaa aggagaguuc     3480

uccaaggcua ccaucaaacc caagggcaac agcucuaaac ugauuccgcg gaagaccaau     3540

ugggauccua ugaaguacgg gggccucgac ucccccaaca uggccuaugc cguggugauc     3600

gaguacgcua aaggaaagaa uaaacugguc uuugaaaaga aaauuauccg cgugacaauu     3660

auggagagaa aggccuucga aaaggaugag aaagcguuuc uggaggaaca gggcuaucgg     3720

cagccuaagg uucuugcuaa acugcccaag uacacgcucu augaguguga agaggggaga     3780

cgccggaugc uggccagcgc caacgaagcu cagaaaggaa aucaacaggu gcucccuaac     3840

caucugguca cccuucugca ccaugccgcu aacugcgagg uguccgacgg caagucucuc     3900

gauuacaucg agagcaauag agaaauguuc gccgagcugc uggcgcacgu uuccgaauuu     3960

gccaagcgcu auacacuugc ugaggccaac cugaauaaaa ucaaccagcu cuuugaacag     4020

aauaaggagg gggacauuaa agcuaucgcc caaucuuucg uggaucugau ggccuuuaac     4080

gcuaugggag cgcccgccag cuucaaguuu uucgagacca caauugaacg gaaaagauac     4140

aauaaccuca aggagcugcu uaauuccacc aucauuuacc agucuaucac gggccuguau     4200

gaaagccgca agcggcugga cgauaaaaga ccggcugcca caaagaaagc cgggcaggcu     4260

aagaaaaaga aauaaggacu aguaauaagg aaacuguaug aaugucugug ggcaggaagu     4320

gaagagaucc uucuguaaag guuuuuggaa uuaugucugc ugaauaauaa acuuuuuuga     4380

aauaauaaau cugguagaaa aaugagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu                            4479


<210>  4786
<211>  4512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4786
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagaa accguacagc      240

auuggacugg acaucggcac caacuccguu gggugggcgg uggugacaga ugacuauaag      300

guccccgcua agaaaaugaa ggugcucgga aauacggaua aaucucauau ugagaagaac      360

cugcuuggcg cccuccuguu cgacagcggg aauaccgccg aagaucggcg ccugaaaaga      420

acagcucggc gcagauacac ccggcgcaga aaccggaucc uuuaucugca ggagauuuuc      480

uccgaagaga ugggaaaggu ugacgauucu uucuuucacc gccucgaaga cagcuuccug      540

gugacagagg auaagagagg cgagcggcau ccuaucuuug gcaaucugga agaggaaguc      600

aaauaccacg agaacuuccc caccaucuau caucuccgcc aauaccuggc cgacaauccu      660

gagaaggugg aucuuagacu gguuuaucuc gcucuggccc acauuaucaa auuucggggg      720

cacuuccuua uugaaggaaa guuugacaca cgcaacaaug augugcagag acuguuccag      780

gaguuucucg cggucuacga caacaccuuc gaaaauuccu cucugcagga gcaaaacguc      840

cagguggaag agauccugac ggacaaaauu agcaaguccg ccaaaaagga ucgggugcuc      900

aagcuguuuc ccaaugagaa aagcaacggc agauucgcug aauuucuuaa gcugaucguu      960

gggaaucagg ccgacuuuaa aaagcauuuc gagcucgaag agaaagcucc ucugcaguuu     1020

ucuaaggaua ccuacgaaga ggagcuugaa gugcugcucg cccaaauugg agacaacuau     1080

gcggagcugu uccuguccgc caagaaacuc uacgauagca uccugcuuuc uggcauccug     1140

acagugaccg acgucgggac aaaggcuccg cucuccgcca gcaugauuca gcgcuauaac     1200

gaacaccaga uggaucuggc ucagcuuaaa caauuuaucc ggcagaagcu gucugacaaa     1260

uacaaugagg uguucuccga uguuagcaag gacggauaug ccggcuacau ugauggcaag     1320

accaaccagg aagccuuuua uaaauaccuc aaggggcugc ugaauaaaau cgagggaucc     1380

ggcuacuucc ucgacaagau ugagagagaa gacuuucugc gcaaacaacg gacguucgau     1440

aacgggucua ucccccauca gauccaccuu caggagauga gagcuauuau ccgccggcag     1500

gcggaauuuu auccuuuccu ggccgacaau caagauagaa uugagaagcu ccugacauuu     1560

cgcauccccu acuauguggg accucuggcu cggggcaaga gcgacuucgc cuggcuuucc     1620

agaaaaagcg ccgauaagau uacccccugg aacuucgacg agaucgucga uaaagaaucu     1680

uccgcugagg ccuuuaucaa ucgcaugaca aacuacgacc ucuaucugcc gaaucagaag     1740

gugcugccua aacacagccu ccuguacgaa aaguucaccg uuuauaacga gcuuaccaaa     1800

gugaaguaca agacagaaca ggggaaaacg gcguuuuucg augcuaauau gaagcaggag     1860

auuuuugacg gagucuucaa aguguaccgg aaggucacca aagauaagcu gauggacuuu     1920

cucgagaagg aguucgauga auuuagaauc guggaccuga caggccugga caaagaaaac     1980

aagguuuuca augccucuua uggcaccuac caugaucuuu gcaaaauucu cgacaaggau     2040

uuucuggaca acagcaaaaa ugagaagauc cuggaagaua uugugcucac acugacccuu     2100

uucgaggacc gggagaugau ccgcaagaga cuggaaaacu auuccgaucu ccugacgaaa     2160

gagcaaguca agaaacugga acggagacac uacacagggu ggggacgccu uucugccgag     2220

cucauucaug gcauccggaa caaggaaagc agaaaaacca uccuggacua ucugauugau     2280

gacggaaauu ccaaccgcaa uuuuaugcag cucaucaacg augacgcucu gagcuucaag     2340

gaggagauug ccaaggcuca ggugaucggg gaaaccgaua aucuuaacca gguugugucu     2400

gacauugccg gcucccccgc gaucaaaaag gggauccugc aaagccucaa aauugucgac     2460

gagcugguga agaucauggg ccaccagccu gaaaauauug ugguugagau ggccagagag     2520

aaccaguuca caaaucaggg acggcgcaac ucucaacaga gacugaaagg ccuuaccgau     2580

uccaucaagg aauuugggag ccagauucuc aaagagcauc ccgucgaaaa uucccaacug     2640

cagaacgacc ggcuguuccu cuacuaucug cagaauggac gcgauaugua cacaggcgag     2700

gaacuugaca ucgauuaccu gucucaguau gacaucgauc acauuauccc ccaggcuuuu     2760

auuaaggaca acagcaucga uaauagagug cucacguccu cuaaggagaa ccgggggaaa     2820

agcgacgaug ugccuuccaa ggacgucgug cgcaaaauga aaagcuacug gucuaaacug     2880

cuguccgcca aacuuauuac ccaaagaaag uucgacaacc ugacaaaagc ugagcgggga     2940

ggccucaccg augacgauaa ggccggguuu aucaaacgcc agcugguuga aacaagacag     3000

aucaccaagc acguggcgcg gauucucgac gagagauuca auacggaaac cgaugagaac     3060

aauaaaaaga uccgccaagu caagauugug acacugaaaa gcaaccuugu uucuaauuuu     3120

cggaaggagu ucgaacugua uaaagugaga gagaucaacg acuaccauca cgcccaugau     3180

gcuuaucuca augccgucau uggcaaggcu cugcugggag uguacccgca gcuugaaccc     3240

gaguuuguuu auggcgacua cccucacuuc cacgggcaua aagaaaacaa ggccaccgcc     3300

aagaaauuuu ucuacuccaa uaucaugaac uucuuuaaga aagaugacgu gcgcacagau     3360

aagaauggag agauuaucug gaaaaaggac gaacacauca gcaacauuaa gaaaguccug     3420

ucuuaucccc aggugaauau cgugaagaaa gucgaggagc agaccggcgg guucagcaag     3480

gaauccauuc ucccuaaagg aaacagcgau aagcugaucc cccggaaaac gaagaaguuu     3540

uacugggaca caaagaagua uggcggguuc gacucuccga uuguugcuua cuccauccuc     3600

gugaucgcgg auauugagaa aggcaagagc aaaaagcuga agaccgugaa agcccuuguc     3660

ggagugacaa ucauggaaaa gaugaccuuu gagagagacc cuguugcuuu ccuggagcgc     3720

aaaggcuauc ggaaugugca agaagagaac auuaucaagc uccccaaaua cucccuguuu     3780

aagcuggaaa acgggagaaa gcgccuucug gccucugccc gggagcucca gaaaggaaau     3840

gaaauugucc ugccuaacca ucucggcacc cugcuuuauc acgcuaagaa uauccauaaa     3900

guggaugagc ccaagcaccu ggacuacguu gauaaacaca aggacgaguu caaggaacug     3960

cucgaugugg ucagcaacuu uuccaaaaag uacacacugg ccgaggggaa ucuugaaaag     4020

aucaaggagc uguaugcgca gaacaaugga gaagaccuca aagagcuggc uucuagcuuc     4080

auuaaccucc ugacguuuac cgccaucggc gccccugcua cauucaaguu uuucgauaaa     4140

aauauugaca gaaagcggua caccuccaca accgagaucc uuaacgccac gcugauucau     4200

caaucuauca cagggcugua ugaaacccgc auugaucuca auaagcuggg cggagacaaa     4260

agacccgcgg cuaccaagaa agccggccag gccaagaaaa agaaguaagg acuaguaaua     4320

aggaaacugu augaaugucu gugggcagga agugaagaga uccuucugua aagguuuuug     4380

gaauuauguc ugcugaauaa uaaacuuuuu ugaaauaaua aaucugguag aaaaaugaga     4440

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaagaa uu                                                         4512


<210>  4787
<211>  3645
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4787
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcccagac aacaaaccug      240

agcuacauuc ucggacugga ccuuggcauc gcguccguug ggugggcugu gguggagauu      300

aaugaaaacg aggauccgau cggacugauu gacgucggcg ugcggaucuu cgaacgcgcc      360

gagguuccca agacggggga aucucucgcc cugagcagac ggcuugcucg cuccaccaga      420

cggcugaucc gcagacgggc ccaucgccuc cugcuggcua aaagauuucu caagcgggag      480

gggauucugu cuacaaucga ucuugagaag ggccugccua aucaagccug ggaacuccgc      540

guggcgggcc uggagagacg gcuuagcgcc auugaauggg gagcuguccu gcuccaccug      600

aucaaacauc gcggauaucu guccaagcgg aaaaacgagu cucagaccaa uaacaaggag      660

cucggcgccc ugcuuagcgg gguggcucag aaucaccagc ugcuccaauc cgacgauuac      720

agaacacccg ccgaacuggc gcuuaaaaag uucgccaagg aggaaggaca uauuagaaac      780

cagcgcggcg cuuauaccca cacauuuaau cggcuggacc uccuggccga gcugaaccuc      840

cuguucgcuc agcagcacca auuugggaau ccucauugca aagaacacau ccagcaguac      900

augaccgagc uucugaugug gcagaagccc gcccucagcg gagaggccau ucugaaaaug      960

cuuggcaagu guacgcauga aaagaacgag uucaaagcug cgaaacacac cuauucugcc     1020

gaaagauuug uuuggcugac aaagcucaau aaccugcgca uccuggagga uggcgcugaa     1080

cgggcccuca augaggagga aagacaacug cuuaucaacc acccuuacga gaaguccaaa     1140

cugaccuaug cccaggugcg caagcuccug gggcugagcg aacaggcuau uuucaaacau     1200

cuucgguacu cuaaggagaa cgccgaaucc gcgacauuua uggagcucaa ggcuuggcac     1260

gccaucagaa aagcccugga gaaucaagga cugaaggaca ccuggcagga ucucgcuaaa     1320

aagccggacc ugcuugauga aauuggcacg gccuucagcc uguacaaaac agacgaggau     1380

auccagcagu aucucaccaa caaggucccc aauucuguga uuaacgcucu gcuggugucc     1440

cuuaauuuug acaaauucau cgaacucagc cugaaguccc ugcgcaagau ccucccucug     1500

auggagcaag ggaaacggua cgaccaggcc ugcagagaga uuuauggaca ucacuacggc     1560

gaagcgaacc agaagacauc ucagcuucug cccgccaucc cugcucaaga gauucgcaau     1620

cccgucguuc uccggacccu gagccaggcc agaaaaguga ucaacgcuau uauccggcag     1680

uauggguccc cggcccgcgu gcauaucgaa accggaagag agcugggcaa gagcuuuaaa     1740

gaacggagag agauucagaa gcaacaggag gauaaucgca caaagcggga aucugcgguc     1800

cagaaauuca aggagcuuuu cuccgacuuu agcucugaac cuaaaagcaa ggauauccuc     1860

aaauucagac uguacgagca gcaacacggc aagugucugu auuccgggaa agaaauuaac     1920

auccaccgcc ucaaugagaa gggauacgug gagauugacc augcccugcc cuuuucuaga     1980

acgugggaug acagcuucaa caauaagguu cuugugcugg cuuccgaaaa ccagaauaaa     2040

ggcaaccaga ccccuuacga guggcuccaa gggaagauca acagcgaacg guggaaaaau     2100

uuugucgccc uggugcuggg aucucagugc uccgcugcca agaaacagcg ccuucucaca     2160

cagguuauug augacaacaa guucaucgau agaaaucuga acgacacccg guauaucgcc     2220

cgcuuucuga gcaauuacau ucaagagaac cuccugcuug ugggcaagaa uaaaaagaac     2280

gucuucacac ccaaugggca gaucaccgcu cugcucagau cucggugggg ccugauuaaa     2340

gcgcgcgaaa acaauaacag acaccaugcc cuggaugcua ucguggucgc cugugccacg     2400

cccuccaugc agcagaagau uacacgguuu auccgcuuca aagaggugca cccuuauaag     2460

aucgagaaua gauacgaaau gguugaccaa gagagcggag aaauuaucuc cccgcacuuu     2520

cccgagccuu gggcuuauuu ccggcaggag gugaacauua gagucuuuga caaucauccc     2580

gauaccgugc uuaaggaaau gcugccugac cgcccccagg ccaaccacca guucguucaa     2640

ccucucuucg ugucucgggc gccgaccaga aaaaugagcg gccaggggca uauggagaca     2700

aucaaguccg cuaaacgccu ggccgaagga auuucugucc uccggauccc ccugacccag     2760

cuuaagccua accugcucga gaauauggug aacaaagaaa gagagcccgc ccuguacgcu     2820

ggccugaagg cccgccuugc ggaguuuaau caagauccug cuaaggccuu cgccacaccc     2880

uuuuauaaac aagggggaca acaggugaag gcuauccggg uugaacaggu ccagaaaagc     2940

ggcgugcugg ugagagagaa caaugggguc gccgacaacg cuuccauugu gcgcacggau     3000

guuuucauca agaauaacaa auuuuuccuc gugccgauuu acaccuggca ggucgccaag     3060

ggcauccugc cuaauaaagc gauuguggcc cacaagaacg aagacgagug ggaagagaug     3120

gaugagggag cuaaguuuaa auucagccuc uuucccaaug accugguuga acuuaagacc     3180

aaaaaggagu acuucuuugg cuauuacauc gggcuggauc gggccacagg aaacaucucu     3240

cucaaagaac augacggcga gauuuccaag gggaaggaug gaguguauag agucggcgug     3300

aaacuggcuc ugagcuucga aaaguaccag guugacgagc uugggaaaaa ucggcagauc     3360

ugccgccccc agcagagaca gccugugcgg aagcgcccgg ccgccaccaa aaaggcuggc     3420

caagcgaaga aaaagaaaua aggacuagua auaaggaaac uguaugaaug ucugugggca     3480

ggaagugaag agauccuucu guaaagguuu uuggaauuau gucugcugaa uaauaaacuu     3540

uuuugaaaua auaaaucugg uagaaaaaug agaucuaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                     3645


<210>  4788
<211>  3723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4788
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgcggc uuucaagccg      240

aaccccauua auuacauccu gggacucgac auuggcaucg ccagcguugg gugggccaug      300

guggagauug augaagacga gaacccuauc ugccugaucg aucuuggagu gcgggucuuu      360

gaacgcgcug aggugcccaa aaccggcgac ucccuggcca uggcuagacg gcucgcccgc      420

ucuguuagac ggcugacacg cagacgggcg caucgccuuc ugagagcccg gcgccuccug      480

aagagagaag gggugcugca ggcugccgau uucgacgaga auggacucau uaagagccug      540

ccuaacacgc ccuggcaacu ucgggcugcc gcgcuggauc gcaaacucac cccucuggag      600

ugguccgccg uccuucugca ccucaucaag cauagaggcu aucugucuca gcggaaaaau      660

gaaggcgaga cagcugacaa ggaacugggg gcccuccuga aaggaguggc ugauaacgcc      720

cacgcccuuc agaccggcga cuuuagaaca ccggcugagc uggcgcucaa uaaguucgag      780

aaggaaagcg ggcauauucg caaccagcgg ggagauuacu cccacaccuu uucuagaaaa      840

gaccugcaag ccgagcuuau ccuccuguuc gaaaagcaga aagaguuugg caauccccac      900

guuagcgggg gacugaagga aggcauugag acacugcuca ugacccagcg cccugcucug      960

uccggcgaug ccgugcagaa aaugcuuggg cauuguacgu ucgagcccgc cgaaccuaag     1020

gcugccaaaa acaccuauac agcggagcgg uuuaucuggc ugaccaagcu caauaaccug     1080

cgcauucuug aacaaggaag cgagagaccc cugacagaca ccgaacgggc uacgcucaug     1140

gacgagccgu acagaaaguc uaaacugaca uaugcccagg cccgcaagcu gcucggccug     1200

gaggauaccg cuuucuuuaa agggcuucgg uacggaaagg acaaugccga ggcuuccaca     1260

cugauggaaa ugaaagccua ucacgcgauc agcagagccc ucgaaaagga gggccugaag     1320

gauaaaaagu cuccucugaa ccuuuccccc gaacuccagg acgagaucgg gaccgcuuuc     1380

agccuguuua aaaccgauga ggacauuaca ggacgccuga aggaucggau ccagccugaa     1440

auucucgagg cccugcuuaa acauaucucu uucgacaagu ucguccaaau uucccugaag     1500

gcucucagac ggaucgugcc ccugauggaa cagggcaaac gcuacgauga ggccugcgcg     1560

gagaucuacg gcgaccacua ugggaagaaa aauacggaag agaagauuua ccugcccccu     1620

aucccggccg augaaauuag aaaccccgug guccuucggg cucucagcca ggccagaaaa     1680

guuaucaacg gaguggugcg ccgguauggc uccccugcuc gcauucacau cgagaccgcc     1740

agagaagucg ggaagucuuu uaaggacaga aaagagaucg agaagcggca agaagagaau     1800

cgcaaagaca gagaaaaggc cgcugcgaaa uuccgggagu acuuucccaa cuucguggga     1860

gaaccuaaga gcaaagauau ucugaagcug cgccucuaug agcagcagca uggcaagugu     1920

cuguacuccg ggaaagagau caaucuuggc agacugaacg aaaagggaua uguugagauu     1980

gaccacgccc uccccuuuag ccggacaugg gaugacucuu ucaauaacaa agugcugguc     2040

cugggcuccg aaaaucagaa caaggggaau caaaccccgu acgaguacuu uaacggaaaa     2100

gauaauagcc gcgaauggca ggaguucaag gcuagagugg agacaucucg guuuccucgc     2160

agcaagaaac agagaauccu ucuccagaag uucgacgaag auggcuuuaa agagcggaac     2220

cugaaugaca ccagauaugu uaaccgcuuc cugugccaau uuguggccga ucggaugaga     2280

cucacgggga agggcaaaaa gcgcgucuuc gccuccaaug gacagauuac aaaccugcuu     2340

cggggguucu ggggccugag aaaggugcgc gcugaaaacg accggcauca cgcccucgau     2400

gcggucgugg uugcuuguuc uaccguggcc augcagcaga aaaucaccag auuuguccgc     2460

uacaaggaga ugaaugccuu cgacggaaaa acaauugaua aggagaccgg cgaagugcug     2520

caucagaaaa cacacuuucc ccagccuugg gaguucuuug cucaggaagu uaugauccgg     2580

guguucggga agcccgacgg aaagccugag uuugaagagg ccgacacgcc cgagaaacug     2640

agaacccuuc ucgcggaaaa gcugagcucc cggccggagg cuguccacga auaugugaca     2700

ccucuguucg ugagccgcgc ccccaacaga aaaaugucug gccaggggca uauggagacc     2760

guuaaguccg ccaaacggcu cgaugaagga gucagcgugc ugcgcgugcc ccuuacacaa     2820

cugaagcuca aagaccugga gaagaugguc aauagagagc gggaaccuaa gcuguacgag     2880

gcucuuaaag ccagacugga agcucacaag gaugacccgg ccaaagcguu ugccgagccc     2940

uucuauaagu acgauaaagc uggcaaccgc acccaacagg ugaaggccgu ucggguggaa     3000

caaguccaga agacgggggu guggguuaga aaucauaacg gcaucgcuga caaugccacc     3060

auggugcgcg ucgauguguu ugagaaagga gacaaguauu accucguucc uauuuacucu     3120

uggcaagugg ccaaaggcau ccugcccgau cgggcugucg ugcaggggaa ggacgaggaa     3180

gauuggcagc ucauugacga uuccuucaac uuuaaauuca gccugcaccc uaaugaccuu     3240

guggagguca ucacaaagaa ggcgagaaug uucggauauu uugccuccug ccaccgcggc     3300

accgggaaca uuaauauccg gauccaugac cuggaucaca aaauuggaaa gaacggcauc     3360

cucgaaggga uuggcguuaa aacagcucug ucuuuccaga aguaccagau cgacgagcug     3420

ggaaaagaga uuagacccug ucgccuuaag aagagaccuc ccgugcggaa acgcccggcc     3480

gccaccaaga aagcuggcca ggccaagaaa aagaaguaag gacuaguaau aaggaaacug     3540

uaugaauguc ugugggcagg aagugaagag auccuucugu aaagguuuuu ggaauuaugu     3600

cugcugaaua auaaacuuuu uugaaauaau aaaucuggua gaaaaaugag aucuaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     3720

auu                                                                   3723


<210>  4789
<211>  3729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4789
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagua ccauguugga      240

auugacgugg gcaccuucag cguggggcug gcggcuaucg aggucgauga cgccggaaug      300

ccgauuaaaa cacucucccu ggugucucac auccaugaua gcggccuuga ccccgaugaa      360

auuaaguccg ccguuacgcg gcuggcuucu agcgggaucg cccgcagaac ccggcgccuc      420

uauagacgga agcgcagacg gcugcagcaa cuugacaaau uuauccagcg ccagggaugg      480

ccugugauug agcuggaaga uuacuccgac ccccucuauc cuuggaaggu cagagcugag      540

cuggccgcgu cuuacaucgc cgaugaaaaa gagcggggcg agaagcugag cguggcucuc      600

cgccacauug ccagacaucg gggcuggcgc aaccccuaug cuaaaguuuc cagccuguac      660

cuuccugacg ggccgucuga ugccuucaag gcgaucagag aagagauuaa gcgggccucc      720

ggacagcccg ugccugaaac agcuaccguc ggccaaaugg ugacacugug cgagcucggg      780

acccugaaac uuagaggaga gggcggggug cugagcgccc gccuccagca gucugacuau      840

gcucgggaaa uccaggagau uuguagaaug caagaaaucg gacaggagcu guaccgcaag      900

aucauugaug ucguuuuugc cgccgaaucc cccaaaggca gcgcuucuuc ccgggugggc      960

aaggacccuc ugcagcccgg gaaaaauaga gcgcucaagg ccagcgacgc uuuccagcgc     1020

uaccggaucg ccgcccugau uggaaaccuu agagugcgcg ucgauggcga gaaacggauc     1080

cuguccgugg aggaaaagaa ucucguuuuu gaccaccugg ugaaccuuac accgaagaaa     1140

gagccugaau gggucaccau ugcugagauc cuggggaucg auagaggaca acucauuggc     1200

acggccacca ugacagacga uggggaacgc gcgggagcuc ggcccccuac ccacgacaca     1260

aauagaucua ucgugaacag ccggauugcu ccccugguug auugguggaa gaccgccucc     1320

gcccuggagc agcaugccau ggugaaagcu cucagcaaug ccgaggucga cgauuucgac     1380

ucucccgaag gcgcgaaggu gcaggccuuu uucgcugauc uggacgacga uguccacgcc     1440

aaacuugacu cccugcaucu cccugugggc cgcgcugccu auagcgagga uacgcugguu     1500

agacuuacac ggagaaugcu gucugacggg guggaucucu acaccgcgcg ccugcaagaa     1560

uuuggaaucg agccgagcug gacacccccu acaccccgga uuggcgaacc ugucgggaac     1620

cccgccgugg acagaguucu gaagaccgug ucccgcuggc ucgagucugc uaccaagacg     1680

uggggagccc cggagagagu caucaucgaa cacgugcggg agggcuucgu gaccgaaaaa     1740

cgcgcuagag agauggaugg ggacaugcgg cgcagagccg cccggaaugc uaagcuguuu     1800

caggagaugc aggaaaaacu uaacguucag ggcaagccua gccgcgcgga ucuguggaga     1860

uaucaauccg uccagcggca gaauugccag ugugccuacu gcggaagccc cauuacauuc     1920

ucuaacuccg agauggacca caucgugccu cgcgcuggcc aagggagcac caauacaaga     1980

gaaaaccucg uggccgucug ucaucggugc aaucagucua aaggaaacac ccccuuugcc     2040

auuugggcua agaacacguc caucgagggc gugagcguua aggaagccgu ggagagaaca     2100

cgccacuggg ucaccgauac cgggaugcgg uccacagacu ucaaaaaguu caccaaagcg     2160

gugguugaga gauuucagcg cgcuacaaug gaugaagaga uugacgcccg gucuauggaa     2220

agcguggccu ggauggcuaa ugagcugaga ucccgcgucg cccagcauuu cgcgucucac     2280

ggaacgaccg ugcggguuua uagaggcagc cugacagcug aagcccgccg ggccuccggg     2340

aucagcggca agcugaaauu uuucgacgga gugggcaagu cuagacucga ucggcgccau     2400

cacgcuauug acgccgcugu caucgccuuu accuccgauu acguggcgga gacacuggcc     2460

gugagaagca accugaagca aucucaggcu caccggcagg aggccccuca guggcgcgaa     2520

uuuaccggga aagacgcuga gcauagagcc gccuggcggg ucugguguca aaagauggaa     2580

aaacucuccg cucugcuuac ggaggaucug agagacgauc gcguuguggu gaugagcaau     2640

guccggcuca gacugggaaa cggcucugcg cacaaggaaa ccaucgggaa acugagcaag     2700

gugaaacuuu ccagccagcu cucuguuucc gacauugaua aggccagcuc cgaggcucug     2760

uggugcgccc ugacacgcga gcccggauuu gacccgaagg aaggccuccc ugccaauccc     2820

gagcggcaua ucagagugaa cgggacccac gucuaugcug gcgauaauau uggacuguuc     2880

cccgugucug ccggcagcau cgcgcuucgc gggggauacg cugaacuggg cuccucuuuu     2940

caccaugccc ggguuuacaa aauuacaagc gggaagaaac cugccuucgc uaugcucaga     3000

guguauacca ucgaccugcu gccguaccgc aaccaggauc uuuucuccgu cgagcucaag     3060

ccccaaacga ugucuaugag acaggccgag aaaaagcugc gggacgcgcu ggcuacagga     3120

aaugccgaau aucucggcug gcuggugguu gacgaugagc uuguggucga caccagcaag     3180

aucgccacag aucaggugaa agcuguggaa gccgagcugg ggaccauucg cagauggcgg     3240

gucgacggcu ucuuuucccc uagcaagcuc cgccugagac cccugcagau gucuaaagaa     3300

ggaaucaaga aagaguccgc uccugagcuu agcaagauua ucgaucggcc cggcuggcuc     3360

ccugccguua acaagcuguu cucugacggg aaugugaccg uggucagacg cgauucccug     3420

ggacggguga gacucgaaag cacagcgcac cuucccguua cguggaaagu gcaaaagcgc     3480

ccggccgcua ccaaaaaggc cggccaggcu aaaaagaaga aauaaggacu aguaauaagg     3540

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa     3600

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

aaaagaauu                                                             3729


<210>  4790
<211>  3429
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4790
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgcgcg gauucuggcu      240

uucgacaucg gaauuagcuc caucggcugg gccuuuucug agaacgauga acucaaggac      300

ugcgggguuc gcauuuucac caaaguggag aauccgaaga caggagaaag ccuggcccuu      360

cccagacggc uggcucgcuc cgccagaaag cggcucgcuc gcagaaaagc ccggcugaac      420

caucuuaagc accugaucgc gaaugaguuu aaacucaacu acgaagauua ucagucuuuc      480

gacgagagcc uggccaaggc uuacaaaggc ucccugaucu cuccuuauga gcuccgcuuu      540

agagcccuga augaacuucu gagcaagcaa gauuucgcuc gggugauucu ccauaucgcc      600

aagcgcagag gguacgacga uauuaaaaac uccgacgaua aggagaaagg agcgauccug      660

aaggccauua aacagaauga agagaaacuu gcuaacuauc agagcgucgg cgaguaccug      720

uauaaagaau acuuucagaa guucaaggaa aauucuaaag aauuuacgaa cgugcggaau      780

aagaaagagu ccuacgaacg cuguaucgcc cagagcuucc ucaaggacga gcugaaacug      840

auuuuuaaga agcaaagaga guucggcuuu ucuuucucca aaaaguucga agaggaaguu      900

cucagcgugg cuuuuuauaa acgggcccug aaggauuucu cucaccuugu cgggaacugc      960

uccuuuuuca ccgacgagaa aagagccccc aagaauagcc cucuggcuuu uauguucgug     1020

gcgcucacac gcaucaucaa ccugcuuaau aaccugaaga acaccgaagg cauucucuac     1080

acaaaagaug accugaaugc ccugcucaac gagguucuga agaauggaac ccuuacauau     1140

aaacagacca agaaacugcu cgggcugucc gacgauuacg aguuuaaggg agaaaagggc     1200

acguauuuca ucgaguuuaa aaaguacaaa gaguucauua aggcucuugg ggagcauaac     1260

cugucucagg acgaucucaa ugaaaucgcc aaagacauua cccugaucaa ggaugagauc     1320

aaacugaaga aggcccucgc uaaauaugac cugaaccaaa aucagauuga uagccuuucc     1380

aagcuggagu uuaaagacca ccucaacauc agcuucaagg cccugaaacu ggugacaccc     1440

cuuaugcucg aaggaaagaa guacgaugag gcguguaaug aacugaaccu gaaagucgcu     1500

auuaaugagg acaagaaaga uuuucucccu gccuucaacg agaccuacua uaaggacgaa     1560

gugacaaauc cggugguccu gcgggccauc aaagaguaca gaaagguucu uaacgcucug     1620

cucaagaaau auggcaaggu gcacaaaauu aauaucgaac uggcccgcga ggugggcaag     1680

aaccauucuc agcgggcuaa aaucgaaaag gagcagaacg agaauuacaa ggccaaaaag     1740

gacgcggaac uggagugcga aaaacuuggg cucaagauua acuccaaaaa uauccugaag     1800

cugagacucu ucaaagagca aaaggaauuu ugugccuaua gcggagagaa gauuaaaauc     1860

ucugaucugc aggacgagaa gaugcuugaa auugaucaca ucuaccccua uagccgcucc     1920

uucgacgauu cuuacaugaa caaaguccug guguuuacca agcagaauca agagaaacuc     1980

aaccagacgc cuuucgaggc uuuuggcaau gacagcgcca aguggcagaa gauugaaguu     2040

cuggcuaaaa accugcccac aaagaaacag aagcggaucc uugauaaaaa uuacaaggac     2100

aaggaacaaa agaacuucaa ggauagaaau cucaacgaca cccgcuauau cgcccggcug     2160

gugcugaauu acacaaaaga uuaucucgac uuucugccuc uuuccgauga cgagaacacc     2220

aagcugaaug acacccagaa agggagcaag guccaugugg aggcgaaguc uggaaugcuc     2280

acauccgccc ugagacacac guggggcuuc agcgcuaaag aucgcaacaa ccaucugcac     2340

cacgccauug acgcuguuau cauugccuac gccaauaacu cuaucgugaa ggcuuuuucc     2400

gauuucaaaa aggaacagga gagcaauucc gcggaacuuu augccaaaaa gauuucugag     2460

cucgacuaca agaacaaacg gaaguuuuuc gagcccuuua gcggguucag acagaaaguc     2520

cuggauaaga ucgacgaaau cuucgugucc aaaccggagc ggaagaaacc uucuggagcu     2580

cugcaugaag agaccuuucg caaggaagag gaguucuauc aaagcuacgg cggcaaggaa     2640

gggguccuca aagcccugga gcuuggaaag auuagaaaag ugaauggcaa gaucguuaaa     2700

aacggggaua uguuucgggu ggacauuuuc aagcacaaga aaacaaauaa guuuuacgcc     2760

guccccaucu auaccaugga uuucgcucug aaagugcucc cuaacaaggc cguugcgaga     2820

uccaaaaagg gagaaauuaa ggacuggauc cugauggaug agaauuacga auuuugcuuc     2880

agccuguaua aagacucucu uauccugauu cagacaaagg auaugcagga gcccgaguuu     2940

guguacuaua acgcuuucac cuccagcacg gucucucuca ucguguccaa acaugacaau     3000

aaguuugaaa cacugagcaa aaaccagaag auucucuuca agaaugccaa cgagaaagaa     3060

gugaucgcca agucuauugg cauccaaaau cugaaaguuu ucgagaagua caucgucagc     3120

gcucuugggg aagugaccaa agccgaguuu cgccagcggg aggacuucaa gaaaaagaga     3180

cccgcggcua ccaagaaagc cggccaggcc aagaaaaaga aauaaggacu aguaauaagg     3240

aaacuguaug aaugucugug ggcaggaagu gaagagaucc uucuguaaag guuuuuggaa     3300

uuaugucugc ugaauaauaa acuuuuuuga aauaauaaau cugguagaaa aaugagaucu     3360

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3420

aaaagaauu                                                             3429


<210>  4791
<211>  3840
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4791
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccagcga ccugguucuc      240

ggacuggaua uuggcaucgg guccguggga gugggcauuc uuaacaaggu caccggggag      300

aucauucaua aaaauucucg gaucuucccg gcggcucagg ccgaaaacaa ucuggugcgc      360

agaacaaacc ggcaaggacg cagacucgcc cggcgcaaga agcacagacg gguucgccug      420

aauagacuuu uugaggaaag cggccugauc acggacuuca ccaaaauuuc caucaaccuc      480

aaucccuacc agcugcgggu gaagggccug acagaugagc ucucuaacga ggagcuguuu      540

auugcucuua aaaauauggu caagcaucgc gggaucagcu aucuggacga ugccuccgac      600

gauggaaacu cuagcguggg cgacuacgcu cagauuguua aagaaaauuc caagcagcuc      660

gaaaccaaga caccugggca aauccagcug gagagauauc agaccuacgg acagcuucgg      720

ggcgauuuca caguggaaaa agacgggaag aaacaccgcc ugauuaacgu cuuucccacc      780

agcgccuaua gaucugaggc gcuccggauc cugcaaacgc agcaggaguu caauccucag      840

aucaccgaug aauuuauuaa cagauaccug gagauccuca caggaaagcg caaauauuac      900

cauggccccg gcaaugaaaa gucccggacc gacuacggga gauaucgcac aagcggagag      960

acccuggaua acauuuucgg cauccuuauu gggaaaugca cguuuuaccc ugacgaguuc     1020

cgggccgcua aggccucuua uacagcucaa gaauuuaacc ugcucaauga ccugaacaau     1080

cuuaccgugc cgacagagac caagaaacug uccaaggaac agaaaaacca gaucaucaau     1140

uacgugaaga acgagaaagc caugggaccc gcgaaacucu ucaaguauau ugccaaacug     1200

cugagcugug augucgcuga caucaagggc uacagaauug auaaaucugg gaaggccgaa     1260

auccacaccu uugaggcuua ucgcaaaaug aagacacucg aaacgcugga cauugagcaa     1320

auggaucggg agacccuuga caagcuggcc uacguucuca cacugaauac cgaaagagag     1380

ggaauccagg aagcccuuga gcacgaguuc gcugauggcu ccuucagcca gaaacaggug     1440

gacgaacugg ugcaauuucg caaggcgaac uccucuaucu ucggcaaagg guggcauaau     1500

uuuagcguca agcucaugau ggagcugauu ccugaacugu acgagacauc cgaggaacag     1560

augaccaucc ucacgcggcu gggaaaacag aagacaaccu cuucuuccaa caagaccaaa     1620

uauauugaug agaaacuucu gacagaagag aucuacaauc ccgugguugc caagagcgug     1680

agacaggcua uuaagaucgu caacgccgcc auuaaagaau auggcgacuu cgauaauauc     1740

gugaucgaga uggcucgcga aaccaacgag gacgacgaga agaaagccau ucaaaagauc     1800

cagaaggcga auaaagauga aaaggacgcu gccaugcuca aagccgcuaa ccaguacaac     1860

gggaaggccg agcugccuca cucuguuuuu cauggacaca aacagcuggc uacaaagauu     1920

cggcuuuggc accaacaggg cgaaagaugc cucuauacgg ggaagaccau cagcauucau     1980

gaucugauca auaacuccaa ucaguucgag guggaccaca uccugccccu cucuauuaca     2040

uuugaugaca gccuggccaa caaaguccuu guguacgcga ccgccaauca ggaaaagggc     2100

caacggacac cguaucaggc ucuggauucc auggacgaug ccuggagcuu ccgcgagcuc     2160

aaggcuuuug ucagagaguc uaagacccug uccaacaaaa agaaggaaua ccugcuuacg     2220

gaggaagaca ucagcaaauu cgaugugcgg aagaaauuua uugagagaaa ucucguugac     2280

acccgcuacg ccucucgggu gguccugaac gcgcugcagg aacauuucag agcccacaag     2340

aucgauacaa aaguguccgu ugugcgcgga caauuuacca gccagcucag acggcauugg     2400

ggcauugaga agacacgcga caccuaucac caccaugcug ucgacgcccu gaucaucgcu     2460

gccuccucuc agcuuaaucu guggaagaaa cagaagaaca cgcucgugag cuacuccgag     2520

gaucaacugc uggacauuga aacaggggag cuuaucucug augacgaaua uaaagagagc     2580

guguucaagg ccccuuacca gcacuucguu gauacccuca aauccaagga guuugaagac     2640

agcauucugu ucucuuauca ggucgauucc aaauuuaaua gaaagaucag cgacgcuaca     2700

auuuacgcga cccggcaggc caagguggga aaagauaagg cugacgagac cuaugugcug     2760

ggcaaaauca aggauaucua cacacaagac ggguacgaug ccuucaugaa aauuuauaag     2820

aaggacaaau cuaaguuucu cauguaccgc caugaccccc agacguucga aaaagucauc     2880

gagccuauuc uggaaaacua ucccaauaag cagaucaacg agaaaggaaa ggaggugccc     2940

uguaauccuu uucuuaagua caaagaggaa cacggcuaua uuagaaagua cuccaaaaag     3000

gggaacggcc cggaaaucaa aagccugaag uauuacgauu cuaagcucgg aaaccacauu     3060

gacaucaccc ccaaagauag caauaacaag guugugcugc aguccgucag cccuuggcgg     3120

gccgacgugu acuucaauaa aacaaccggc aaguaugaga uccuggggcu uaaauacgcu     3180

gaucuccaau uugaaaaggg aacaggcacc uauaagauuu cucaggagaa auacaacgac     3240

aucaagaaaa aggagggggu ugauuccgac agcgaguuca aauuuacgcu guauaagaau     3300

gaucugcucc uggugaaaga cacagaaacc aaggaacagc aacuuuuccg cuuucugucc     3360

agaaccaugc ccaagcagaa acauuacguc gagcucaagc cuuaugauaa acagaaguuc     3420

gaaggaggcg aggcccugau uaaagugcug gggaacguug cgaauagcgg ccagugcaag     3480

aagggacuug gcaaaagcaa caucuccauu uacaaggugc ggacagacgu ccuggggaau     3540

caacacauca ucaaaaacga gggagacaag cccaaacucg auuucaagcg cccggcugcc     3600

accaagaaag ccggccaggc uaagaaaaag aaauaaggac uaguaauaag gaaacuguau     3660

gaaugucugu gggcaggaag ugaagagauc cuucuguaaa gguuuuugga auuaugucug     3720

cugaauaaua aacuuuuuug aaauaauaaa ucugguagaa aaaugagauc uaaaaaaaaa     3780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3840


<210>  4792
<211>  4398
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4792
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaccca guucgaggga      240

uuuacaaacc uguaccaagu uagcaagacg cuccgguucg aacugauucc gcagggcaaa      300

acccuuaagc auauccagga gcagggguuu auugaagagg acaaggcgcg caaugaucac      360

uauaaagaac ugaagcccau cauugacaga aucuacaaaa cauaugcuga ucaaugccuc      420

cagcuggugc agcuugacug ggagaaccug uccgccgcca ucgauucuua ccggaaggag      480

aaaaccgaag agacacgcaa ugcucucauu gaagaacagg ccaccuauag aaacgcuauc      540

caugacuacu ucauuggacg gacagauaau cugaccgacg ccaucaacaa gcgccacgcg      600

gagauuuaua agggccuguu uaaagccgaa cucuucaaug ggaaggugcu gaaacaacuu      660

ggaacgguca ccacaaccga gcaugaaaac gcucugcuca gauccuuuga uaaguucaca      720

accuacuuua gcggcuucua cgagaaucgg aaaaacgugu uuucugccga agacaucagc      780

acggcuauuc cucaccgcau cguucaggau aauuucccca aguuuaaaga gaacugucac      840

aucuucacaa gacugauuac cgccgugccu ucccuucggg agcauuucga aaaugucaag      900

aaggcgaucg gcauuuuugu gucuacaagc aucgaggaag uuuucuccuu ucccuucuau      960

aaccagcugc ucacccagac ccaaauugac cuguacaauc agcugcucgg gggaaucagc     1020

cgcgaggccg gcacagagaa aaucaagggg cugaacgagg ugcuuaaccu ggcuauucag     1080

aaaaaugaug agacggccca caucauugcu ucucucccuc auagauuuau cccgcuguuc     1140

aagcaaauuc uuuccgaccg gaacacccug agcuuuaucc ucgaagaguu caaaucugac     1200

gaagagguca uccaguccuu uugcaaguau aagacacugc ugcgcaauga aaacgugcuc     1260

gagaccgccg aggcccuguu caaugaacuu aacagcauug aucugacaca caucuuuauu     1320

ucucacaaaa agcucgagac caucuccagc gcucugugug accauuggga uacgcuuaga     1380

aaugcgcugu acgaacggag aauuuccgag cucacaggaa aaaucaccaa gucugccaaa     1440

gagaaggugc agcgcagccu gaagcacgaa gacauuaacc ugcaggagau caucuccgcu     1500

gccggcaaag aacucagcga ggccuucaag cagaaaaccu cugaaauucu gucccaugcu     1560

cacgccgcgc uugaucaacc ccugccuaca acccucaaga aacaggagga gaaggaaauc     1620

cuuaagagcc agcuggacuc ucuucucggg cuguaucauc ugcucgauug guuugcuguc     1680

gacgagagca augaaguuga ucccgaguuc uccgcccggc ugacaggaau uaaacuugaa     1740

auggagccuu cucugagcuu cuacaacaag gccagaaauu augcuacgaa aaagcccuac     1800

uccguggaga aauuuaagcu caacuuccaa augccgaccc uggccagcgg cugggacgug     1860

aauaaagaaa agaacaaugg cgcuauccug uuugucaaga acgggcuuua cuaucucgga     1920

auuaugccua aacagaaggg ccgcuacaaa gcccugucuu ucgagcccac agaaaagacc     1980

uccgaggggu uugauaaaau guauuacgac uauuucccug acgcggccaa gaugaucccc     2040

aagugcagca cacagcugaa agcugugacc gcccacuuuc agacgcacac cacacccauc     2100

cuccugucua acaauuucau ugaaccucuu gagaucacca aggagauuua cgaucugaac     2160

aauccggaaa aagagcccaa gaaauuucaa acagcuuaug ccaagaagac cggagaccag     2220

aaaggcuacc gggaagcgcu cuguaagugg aucgauuuca cgagagacuu ucuguccaaa     2280

uacacaaaga ccacaagcau ugaucugucc ucucuucgcc cuagcuccca guauaaagac     2340

cucggggagu acuaugccga gcugaacccc cugcucuacc auaucucuuu ccagcggauc     2400

gcugaaaagg agauuaugga ugccguugaa accggaaagc uguaucuuuu ucaaaucuac     2460

aauaaagacu ucgcuaaggg ccaccauggc aaaccuaacc ugcacacccu cuauuggaca     2520

gggcuguuca gccccgagaa ucuggccaag acguccauua aacuuaacgg acaggccgaa     2580

cucuuuuaca gaccuaagag ccgcaugaaa cggauggcuc acagacuggg cgagaagaug     2640

cugaauaaga aacucaagga ucagaaaacc ccgauccccg acacacugua ccaagagcuu     2700

uaugauuacg ugaaccaucg ccugucucac gaccucuccg augaagcgcg ggcccugcug     2760

ccuaauguca uuaccaagga ggugagccau gaaaucauca aagacagacg guucacaucu     2820

gacaaguuuu ucuuucacgu ucccauuacc cuuaacuauc aggcugccaa uuccccuagc     2880

aaguucaacc agcgcgugaa ugccuaccug aaagagcauc ccgaaacgcc gaucauuggg     2940

aucgauagag gagagcggaa ccucauuuau aucacaguca uugacucuac cggcaagauc     3000

cuggagcaga gaagccucaa caccauccaa caguuugauu accagaaaaa gcuggacaau     3060

cgcgaaaaag agcggguggc ugccagacag gcgugguccg ucguggggac aauuaaggau     3120

cuuaagcaag gcuaucugag ccagguuauc cacgaaauug uggaccucau gauccacuac     3180

caggcugucg ugguucugga gaaccugaau uucggauuua aaucuaagcg caccggcauu     3240

gccgaaaaag ccguguacca gcaauucgag aagaugcuua ucgauaaacu gaacugccuc     3300

guccugaagg acuauccugc ugagaagguu gggggagugc ucaaucccua ccagcugaca     3360

gaucaguuua cguccuucgc caaaaugggc acccaaagcg gguuccuguu uuaugugccc     3420

gcgccuuaca cauccaagau cgacccgcug accggauucg ucgaucccuu uguguggaaa     3480

acaauuaaga accaugaauc uagaaaacac uuccucgagg gcuuugacuu ccugcauuau     3540

gaugugaaga ccggggacuu uauccugcac uucaagauga aucggaaccu uagcuuucag     3600

cgcggccugc cuggauucau gcccgcuugg gacauugucu uugagaaaaa ugaaacgcag     3660

uucgaugcca agggcacccc uuuuaucgcc gggaaaagaa uugugcccgu uaucgagaac     3720

caucgguuca caggacgcua cagagaccuc uauccugcua augaacugau cgcccuccug     3780

gaggaaaagg gcauuguguu ccgggauggg uccaacaucc uucccaaacu gcuggagaau     3840

gacgauucuc acgcuauuga caccaugguc gcccucaucc gcagcgugcu gcagaugaga     3900

aacuccaaug cggccacagg agaagauuac auuaacucuc cgguucggga ccuuaacggc     3960

guguguuuug auagccgcuu ccaaaauccu gaguggccca uggacgcuga ugccaacggg     4020

gcuuaccaca ucgcccugaa gggccagcuc cugcucaauc aucugaaaga guccaaggac     4080

cuuaagcugc agaacggaau uagcaaucag gacuggcugg ccuauaucca agaacucaga     4140

aacaaacggc cugcugcgac caagaaagcc ggccaggcua agaaaaagaa guaaggacua     4200

guaauaagga aacuguauga augucugugg gcaggaagug aagagauccu ucuguaaagg     4260

uuuuuggaau uaugucugcu gaauaauaaa cuuuuuugaa auaauaaauc ugguagaaaa     4320

augagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaagaauu                                                   4398


<210>  4793
<211>  4377
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4793
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccagcau uuaccaggag      240

uucguuaaca aguauucccu gucuaaaacc cuccgguuug aacugauccc gcaaggaaag      300

acacuugaga auauuaaggc gcgcggccug auccucgacg augaaaagag agcuaaggac      360

uacaaaaagg ccaaacagau uaucgauaag uaucaucagu ucuuuaucga ggaaauucug      420

agcuccgugu gcaucucuga ggaccuucug cagaacuaca gcgaugugua uuucaagcuc      480

aaaaaguccg acgaugacaa ucugcaaaaa gauuuuaagu cugccaaaga cacgauuaag      540

aaacagauca gcgaguacau uaaggauucc gaaaaguuca aaaaccuguu uaaucagaac      600

cucaucgacg cuaagaaagg gcaggagagc gaucugauuc uuuggcugaa gcaaucuaaa      660

gacaauggaa ucgaacucuu caaggccaac uccgacauca ccgauauuga cgaggcucug      720

gagaucauua agagcuuuaa aggcuggaca accuauuuca agggguuuca cgaaaaucgg      780

aaaaacgucu acucuuccaa ugauaucccc acaagcauua ucuaccgcau cguggacgau      840

aaccuuccua aguuccugga gaauaaagcc aaguaugaau cucucaagga caaagcgccc      900

gaggccauua acuacgaaca gaucaagaaa gaucuggcug aggagcugac cuuugacauu      960

gauuauaaga cauccgaagu uaaucagaga guguucagcc ucgacgaggu cuucgaaauc     1020

gccaacuuua acaauuaccu gaaccagucc ggaauuacca aauucaauac gaucaucggc     1080

ggcaaguuug ugaacgggga gaauaccaag cggaaaggaa uuaacgaaua uaucaaucuu     1140

uacucucaac agauuaacga uaagacacug aaaaaguaua aaaugagcgu ucucuucaag     1200

cagauccugu ccgacaccga gagcaaaucu uuugugauug acaaacuuga ggaugacucc     1260

gaugucguga caaccaugca gagcuucuac gaacaaaucg cugccuuuaa gacgguggag     1320

gaaaaaucua uuaaggagac acugagccuc cuguucgacg aucugaaagc gcagaagcuc     1380

gaccugucca aaaucuacuu uaagaaugau aagucucuua ccgaccugag ccagcaaguc     1440

uucgaugacu auuccguuau cggcacagcc gugcucgaau acauuaccca gcagaucgcu     1500

ccuaaaaacc uggauaaucc cagcaagaaa gagcaggagc uuauugccaa gaaaaccgaa     1560

aaggcuaagu aucugucucu cgagacaauc aaacuggccc uggaagaguu uaacaagcau     1620

cgcgacauug auaaacaaug uagauucgag gaaauccucg ccaauuuugc ugcgaucccu     1680

augauuuucg acgagaucgc ccagaacaag gacaaucugg cucagauuuc caucaaauac     1740

cagaaccaag ggaagaagga ucuucugcag gccagcgccg aagacgaugu gaaagcuauu     1800

aaggaccucc uggaucagac gaacaaucug cuucacaaac ucaagaucuu ccauaucucu     1860

caguccgagg acaaagccaa cauucuggau aaggacgaac acuuuuaucu ggucuucgag     1920

gagugcuacu uugaacucgc gaauaucgug ccgcuguaua acaaaauucg gaauuacauc     1980

acccaaaagc ccuacagcga ugagaaguuc aaacuuaacu uugaaaauuc cacacuggcu     2040

aacggauggg acaagaauaa agagccugau aacaccgcca uucucuucau caaggacgau     2100

aaauauuacc ugggcguuau gaauaagaag aacaauaaaa ucuuugacga caaggccauu     2160

aaagaaaaca agggggaggg auauaaaaag aucguguaca agcugcuucc cggcgcuaau     2220

aaaaugcucc cuaaggucuu cuuuucugcc aaaagcauua aguucuauaa ccccuccgag     2280

gauauccugc gcauuagaaa ccacucuaca cauaccaaaa auggcagccc gcagaagggg     2340

uacgaaaagu uugaguucaa caucgaagac ugucggaaau uuauugauuu cuauaagcag     2400

uccaucagca aacacccuga guggaaggac uucggauuuc gcuucucuga uacgcaaaga     2460

uacaauucca ucgacgaauu uuaccgggag guggagaacc agggcuauaa acugacauuc     2520

gaaaauauua gcgagucuua caucgauucc guugugaacc aggggaagcu cuaucuguuu     2580

cagauuuaca auaaggacuu cagcgcuuau ucuaaaggac gccccaaccu ucauacccug     2640

uacuggaagg cccucuuuga ugaaagaaau cugcaagacg ucguguauaa acugaacggc     2700

gaggcggagc uuuucuaccg gaagcagagc aucccuaaaa agauuaccca ccccgccaaa     2760

gaagcuaucg ccaauaagaa caaggauaau cccaaaaagg aguccgucuu ugaauacgac     2820

cucaucaaag acaagagauu cacagaggau aaauuuuucu uccacugccc uauuaccauc     2880

aacuuuaaga gcucuggggc uaauaaguuc aacgacgaaa uuaaccugcu gcucaaagag     2940

aaggccaaug augugcauau ccuguccauu gaccgcggag agcggcaccu ugcguauuac     3000

acacugguug auggcaaagg caacaucauc aagcaggaca cguuuaauau uaucgggaac     3060

gauagaauga aaaccaauua ucaugacaag cucgccgcua uugaaaagga ucgcgacagc     3120

gcccggaaag auuggaagaa aaucaacaau auuaaggaga ugaaagaagg auaccugucc     3180

cagguggucc acgagaucgc uaagcuggug auugaauaua acgccaucgu uguguucgag     3240

gaccuuaauu uuggcuucaa gagagggcgc uuuaaagucg agaagcaagu guaccagaaa     3300

cucgaaaaga ugcugaucga gaaacugaac uaucucgugu ucaaggauaa ugaauuugac     3360

aaaacaggag gcguucugcg ggccuaccag cuuaccgcuc cguucgagac auuuaagaag     3420

auggggaaac agaccggcau uaucuacuau guccccgcgg gauucacguc uaagauuugu     3480

ccugugaccg gcuuugugaa ccaacuguac cccaaauaug aaagcgucuc caagucucag     3540

gaguucuuca gcaaauuuga caagaucugc uacaaucucg auaaggggua uuucgaguuu     3600

uccuucgacu acaaaaacuu uggagauaag gccgcuaaag gcaaguggac aauugccucu     3660

uucgggagca gacugaucaa uuuucgcaac uccgacaaaa aucauaacug ggauacccgg     3720

gaaguguauc cuacaaagga gcuggaaaaa cuucugaaag acuacagcau cgaguacgga     3780

cacggcgagu guauuaaggc cgcuaucugc ggggaaucug auaaaaaguu cuuugccaaa     3840

cucaccuccg uucugaacac gauucuccag augagaaaua gcaagacagg caccgagcug     3900

gacuaucuua ucucucccgu ggcggauguc aacggaaauu ucuuugacuc ccgccaagcu     3960

ccgaagaaca ugccucagga ugccgacgcc aauggcgcuu accacauugg gcugaaagga     4020

cucaugcugc ugggccggau caagaacaau caggaaggga aaaagcugaa ccuugugauc     4080

aaaaaugagg aauauuucga guuuguucag aacagaaaua acaagcggcc cgccgcgaca     4140

aagaaggcug gacaagccaa gaaaaagaaa uaaggacuag uaauaaggaa acuguaugaa     4200

ugucuguggg caggaaguga agagauccuu cuguaaaggu uuuuggaauu augucugcug     4260

aauaauaaac uuuuuugaaa uaauaaaucu gguagaaaaa ugagaucuaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaauu        4377


<210>  4794
<211>  4517
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4794
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgacaa gaaauacagc      240

auuggacugg auaucggcac caacuccguu gggugggcgg ugauuacaga cgaguauaag      300

gugccgucua agaaauucaa gguccucgga aauacggauc ggcauagcau caaaaagaac      360

cugauuggcg cucugcuguu ugacuccggg gaaaccgccg aggccacacg ccucaaaaga      420

accgcucggc gcagauacac acggcgcaag aauagaaucu gcuaucugca ggaaaucuuc      480

ucuaacgaga uggccaaggu ggaugacagc uuuuuccacc ggcuugaaga guccuuucug      540

guugaggaag auaaaaagca ugagcgccac cccauuuucg gaaauaucgu ggacgaaguc      600

gcuuaccaug agaaauaucc uaccauuuac caccucagaa agaaacuggu ggauucuaca      660

gacaaggccg aucugcggcu caucuaucug gcgcuugccc acaugauuaa auuucgcggc      720

cauuuccuga ucgagggcga ccucaacccc gauaauagcg acguugacaa gcuguuuauu      780

caacuugugc agaccuacaa ccagcuguuc gaagagaauc cuaucaacgc uuccgggguc      840

gaugccaagg cuauccucag cgccagacug ucuaaauccc ggcgccugga aaaucucauu      900

gcgcagcugc ccggagagaa gaaaaacggc cuuuuuggga aucugaucgc ccucagccug      960

ggacuuacgc cuaacuucaa gucuaauuuu gaccuggcug aagaugccaa acuccaacug     1020

uccaaggaca ccuacgauga cgaucuggac aaccuccugg cucagauugg cgaucaguau     1080

gccgaccuuu uccuggccgc uaagaaccuc agcgaugcga uccugcuuuc ugacauucug     1140

agagugaaua cagagaucac caaagccccg cucuccgcua gcaugaucaa gcgguacgac     1200

gagcaccauc aggaucugac acugcucaaa gcccugguga gacaacagcu ucccgaaaag     1260

uauaaagaga uuuucuuuga ccaguccaag aacggguacg ccggauauau cgauggcggc     1320

gcuucucagg aagaguucua caaguuuauu aaaccuaucc uggaaaagau ggacgggacc     1380

gaggagcucc uggucaaacu gaaucgcgaa gaucuucucc ggaagcaaag aacguucgac     1440

aacggaagca uuccccacca gauccaccug ggcgagcugc augccauccu ccgccggcag     1500

gaagauuuuu auccuuuccu gaaagacaau agagagaaga uugaaaagau ccuuacauuu     1560

cgcauucccu acuacguugg gccgcuggcg cggggaaacu ccagauucgc uuggaugacc     1620

cgcaaaagcg aggagacaau caccccuugg aauuuugaag agguggugga uaagggcgcc     1680

ucugcccaau ccuucauuga acggaugacc aacuuugaca aaaaucuccc caacgagaag     1740

guccugccua aacacagccu gcuuuaugag uacuucacag uguauaauga acucacgaag     1800

guuaaauacg ugaccgaggg gaugagaaag cccgcuuuuc ugucuggaga acagaagaaa     1860

gccaucgucg aucuucucuu caagacaaac cgcaaaguga ccguuaagca gcugaaagag     1920

gacuauuuca agaagauuga auguuuugau agcguggaga ucuccggcgu cgaggaccgg     1980

uucaaugcuu cucuuggcac auaccaugac cugcucaaaa ucauuaagga uaaagacuuu     2040

cuggauaacg aagagaauga agacauccug gaggauauug ugcuuacccu cacgcuguuc     2100

gaagacagag agaugaucga ggaacggcug aagacauaug cccaccucuu ugaugacaaa     2160

gucaugaagc agcugaagcg cagacgguac accggguggg gaagacuuag ccgcaaacug     2220

auuaacggca uccgggauaa gcaauccggg aaaaccaucc ucgacuuccu uaagagcgau     2280

ggauuugcga acagaaauuu caugcagcug auucaugacg auucucugac auuuaaagag     2340

gacauccaga aggcccaggu guccggccaa ggggacagcc uccacgaaca cauugcuaac     2400

cuggccggcu cucccgcuau caagaaaggc auucugcaga ccguuaaggu ggucgaugag     2460

cucgugaaag uuaugggacg ccauaagccu gaaaauaucg ugaucgagau ggccagagag     2520

aaccagacaa cgcagaaagg gcaaaagaac ucccgggaac gcaugaaaag aauugaggaa     2580

ggaaucaagg agcugggcag ccagauucuu aaggagcacc cggucgaaaa uacccagcug     2640

cagaaugaga aacucuaccu guauuaccug caaaacgggc gggacaugua uguggaucag     2700

gaacuugaca ucaaucgccu guccgauuac gacguggauc auauuguucc ccagucuuuc     2760

cucaaggacg auagcaucga caacaaaguc cugacaagau ccgauaagaa ucggggaaaa     2820

ucugacaacg ugccuagcga ggaagugguc aagaagauga aaaauuauug gcgccaacuc     2880

cugaacgcga aacuuaucac ccagagaaaa uuugacaauc ugacaaaggc cgagcggggc     2940

gggcucuccg agcuggauaa agcuggcuuc auuaagcgcc agcuggugga aaccagacag     3000

aucacgaagc acguugccca aauucuugac agccggauga acaccaaaua cgaugagaac     3060

gacaagcuga ucagagaagu gaaagucauu acacucaagu cuaaacuggu guccgauuuu     3120

cgcaaggacu uccaguucua uaagguucgg gagaucaaua acuaccacca ugcucacgau     3180

gccuaccuca augccguggu cggaaccgcu cugauuaaaa aguaucccaa acuugaaagc     3240

gaguuugugu acggcgacua uaagguuuac gaugugagaa aaaugaucgc gaagucugag     3300

caggaaaucg ggaaggccac agcuaaauau uucuuuuacu ccaacauuau gaauuucuuu     3360

aagaccgaga ucacgcuggc caacggagaa auucgcaaac ggccucucau cgagacaaau     3420

ggcgaaaccg gggagauugu cugggacaag ggaagagauu ucgccacagu gcgcaaagug     3480

cugagcaugc cccaggucaa caucguuaag aaaaccgagg ugcaaaccgg cggguuuucu     3540

aaggaaagca uccugccgaa gagaaauucc gacaaacuua uugcucggaa gaaagauugg     3600

gacccuaaga aauauggcgg auucgacagc cccacagugg ccuacucugu ccuggugguu     3660

gcgaaggugg agaagggcaa auccaagaaa cucaagagcg ucaaagaacu gcucgggauc     3720

acgauuaugg agcgcuccuc uuuugagaag aacccuaucg auuuccugga agcuaaggga     3780

uacaaagagg ugaagaaaga ccuuauuauc aagcugccca aauauagccu guuugaacuc     3840

gagaauggcc ggaagagaau gcuggccucc gccggggaac uucagaaggg aaacgagcug     3900

gcucucccuu cuaaauacgu uaauuuccug uaucucgcca gccauuacga gaagcugaaa     3960

ggcucccccg aagauaacga gcagaagcag cuuuucgugg aacaacacaa acauuaucug     4020

gacgagauca uugaacagau cucugaguuu agcaagcggg ucauucuggc ggaugcuaau     4080

cucgacaaag ugcuguccgc cuacaacaag caccgcgaua agccgaucag agagcaggcc     4140

gaaaacauua uccaccuuuu cacccugaca aaucucgggg cuccugccgc uuuuaaauau     4200

uucgacacca caauugaucg gaagcgcuac accagcacga aagagguucu ggacgccaca     4260

cucauccauc aaucuaucac cggccuguac gaaaccagaa uugaucuuuc ccagcuggga     4320

ggcgacaagc ggcccgcggc cacaaagaag gcugggcagg ccaagaaaaa gaaauaagga     4380

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4440

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aagaauu                                                    4517


<210>  4795
<211>  5300
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4795
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaacuu caagauucug      240

ccgaucgcga uugaccucgg aguuaaaaau acaggcgugu uuagcgcuuu cuaccagaag      300

gggacauccc uggagcggcu ugauaacaag aauggaaaag uguaugaacu gucuaaggac      360

agcuacacgc uccugaugaa caaucgcacc gccagacggc aucaacgcag aggcaucgau      420

cggaaacagc uugucaagcg ccuguuuaaa cucauuugga cagagcagcu gaaccuggaa      480

ugggacaagg auacccagca agccaucucc uuccucuuua auagacgggg guucucuuuu      540

aucacagacg gauauagccc cgaguaccug aacauugugc cugaacaggu uaaggcuauc      600

cuuauggaua uuuucgacga uuauaauggc gaggacgauc uggacuccua ccucaaacug      660

gccaccgagc aggaaucuaa gaucagcgag auuuauaaca aacuuaugca gaagauccug      720

gaauuuaaac ucaugaagcu gugcacagau auuaaagacg acaagguguc caccaagacg      780

cugaaagaga ucaccagcua cgaguucgaa cuccuggcug auuaccuugc caauuauucu      840

gagucccuga agacacaaaa auuuagcuac accgacaagc agggcaaccu caaagaacug      900

ucuuauuacc accaugauaa guauaauauc caggaguucc uuaagcgcca cgcgacaauu      960

aacgacagaa uccuggauac ccuccugacg gacgaucugg acauuuggaa uuuuaacuuc     1020

gaaaaauucg auuuugacaa gaacgaggag aaacuccaga aucaagaaga uaaggaccau     1080

auccaggccc aucugcacca cuucgucuuu gcugugaaca aaauuaaguc cgagauggcc     1140

agcgggggac ggcaccgcuc ucaguacuuc caagaaauca caaauguucu ugacgagaac     1200

aaucaucagg aaggcuaucu gaagaacuuu ugugagaauc uccacaacaa aaaguacucc     1260

aaucugagcg ugaaaaaccu ugucaaucug aucgggaacc ucuccaaucu ggagcugaag     1320

ccccucagaa aauacuucaa cgauaagauu cacgcuaagg ccgaccauug ggaugaacag     1380

aaauuuaccg agacauauug ccacuggauc cugggagaau ggcggguggg cgugaaggac     1440

caggauaaaa aggacggggc gaaauacucu uauaaggauc uuuguaauga gcugaaacaa     1500

aaggucacca aggccggacu cguugacuuc cugcuggaac uugauccuug ccgcaccauu     1560

cccccuuacc ucgacaacaa caauagaaaa ccgcccaagu gucagagccu gauccugaac     1620

ccuaaauuuc ucgauaauca guaucccaac uggcagcaau accugcagga gcuuaagaaa     1680

cugcagucca uucagaauua ucucgacagc uucgagacag aucugaaggu gcugaagucu     1740

uccaaagacc aaccuuacuu uguggaauac aagagcucua accagcagau cgcuagcggc     1800

cagcgggacu auaaagaucu ugacgccaga auccuccaau ucauuuuuga ucgcgucaag     1860

gcuuccgacg agcugcugcu caaugaaauc uacuuccagg ccaaaaagcu gaagcagaaa     1920

gccucuagcg agcuugagaa gcuggaaucc agcaaaaagc ucgaugaggu gauugcuaac     1980

ucucaacugu cccagauccu gaaaagccag cauacgaaug gcauuuucga acaggggacc     2040

uuucuucacc ucguuugcaa guauuacaag caacggcaga gagcgcgcga cucucggcug     2100

uauaucaugc ccgaguacag auaugauaaa aagcugcaua aauacaacaa uacaggacgc     2160

uucgacgaug acaaccagcu ccugaccuac uguaaucaca agccgcggca gaaaagauau     2220

caacuucuga acgaucucgc cggcgugcug caggucuccc cuaauuuucu gaaggacaaa     2280

auugggagcg augacgaccu uuucaucucc aaguggcucg uggaacacau ccgcggauuu     2340

aagaaggcuu gcgaggauuc ucugaaaauu cagaaagaca accggggccu gcucaaccau     2400

aagaucaaua uugccagaaa cacaaaaggg aagugugaga aggaaaucuu caaucugauu     2460

ugcaaaaucg agggaagcga agauaagaaa ggcaacuaca agcacggccu ugccuaugag     2520

cuggggguuc uccuguuugg agaacccaau gaggcuucca aaccugaguu cgaccgcaag     2580

aucaagaaau uuaacucuau uuacagcuuc gcccagaucc aacagauugc guuugcugaa     2640

cggaagggca augccaacac cugugccgug ugcuccgcug auaaugccca uagaaugcag     2700

caaaucaaaa uuacggagcc cgucgaagac aacaaggaua aaaucauccu gagcgcuaag     2760

gcccagcggc uucccgcgau uccuacacgc aucguggacg gggccgucaa gaaaauggcu     2820

accauucugg ccaagaauau cguggaugac aacuggcaga auauuaaaca gguucucucu     2880

gcuaagcacc aacugcacau cccgaucauu accgagucca acgccuucga auuugagccc     2940

gcgcucgccg augugaaagg aaagagccug aaggacagac ggaaaaaggc ucuugagaga     3000

aucucuccug aaaauauuuu caaagacaag aacaaccgca ucaaagaguu cgccaagggc     3060

auuuccgcuu auagcggggc caaucugaca gauggcgacu uugauggagc caaagaggaa     3120

cucgaccaua ucauuccccg gucucacaag aaguacggca cccugaacga ugaggcuaau     3180

cugaucugcg ucacaagagg ggacaacaaa aauaagggaa accgcaucuu cugucuuaga     3240

gaucuggcgg acaauuauaa acucaagcag uuugaaacga ccgaugaccu ggagauugag     3300

aaaaagaucg ccgauacaau uugggacgcu aacaagaaag auuucaaguu uggcaauuac     3360

cggagcuuca ucaaccucac cccucaggaa cagaaagccu uucgccaugc ccuguuccuu     3420

gcugacgaaa aucccauuaa gcaagccgug aucagagcga ucaacaaucg gaaccgcaca     3480

uuuguuaaug ggacccagag auacuucgcu gaggugcugg ccaacaauau uuaucuccgg     3540

gccaaaaagg agaaccugaa cacggacaag aucuccuuug auuacuucgg cauuccgacc     3600

aucggaaaug ggcgcggcau ugcugaaauc agacagcugu augagaaagu cgacagcgau     3660

auccaggccu acgcgaaggg agacaaaccu caggcuucuu auucccaccu uauugaugcc     3720

augcuggccu ucugcaucgc ugccgacgaa caucggaacg auggcagcau ugggcucgag     3780

aucgacaaga auuacucccu guauccccuc gauaaaaaca caggagaagu guuuaccaag     3840

gacauuuucu cucaaaucaa gauuacagau aaugaguuua gcgacaaaaa gcuggugcgc     3900

aaaaaggcua ucgagggcuu caacacccac agacagauga cgcgggaugg gaucuacgcc     3960

gaaaauuacc uuccuauucu gauccacaaa gagcugaacg aaguuagaaa gggauauaca     4020

uggaaaaauu ccgaggaaau uaagaucuuu aagggcaaaa aguacgacau ucagcaacuc     4080

aacaaucugg ucuauugucu uaaauucgug gacaagccca ucucuaucga uauucagauc     4140

agcacccugg aggagcuccg caacauucug acaaccaaua acaucgcggc caccgcugaa     4200

uacuauuaca uuaaucucaa aacacagaag cugcaugagu auuacaucga aaacuacaac     4260

acggcccuug gguauaagaa auacuccaag gagauggagu uucugcgguc ucuggcuuau     4320

agaagcgaac gcgugaaaau caaguccauu gacgauguca aacaagugcu cgacaaggau     4380

agcaauuuca ucauuggcaa gaucacccug ccuuuuaaaa aggaguggca gcggcuuuac     4440

agagaauggc agaacacaac cauuaaagac gauuaugagu uccugaaguc uuucuucaau     4500

guuaaaucca ucacaaagcu ccacaagaaa gugcgcaagg acuuuagccu gcccauuucu     4560

accaacgaag gaaaauuccu cgucaagcgg aaaacguggg auaauaacuu caucuaccag     4620

auccugaaug acuccgauag cagagccgac ggcacaaagc cguuuauucc ugccuucgac     4680

aucucuaaaa acgagauugu ggaggcuauc auugauuccu uuaccagcaa gaauaucuuc     4740

uggcuuccca agaacaucga acugcaaaaa guugacaaua agaacauuuu ugcgaucgau     4800

accuccaaau gguucgaggu ggaaacaccc agcgaccugc gcgauauugg gaucgccacc     4860

auucaguaua agaucgacaa uaacucucgg ccuaaaguca gagugaagcu cgauuacguu     4920

aucgacgaug acagcaagau uaauuacuuu augaaccauu cccugcuuaa aucucgguau     4980

ccggauaagg ugcuggagau ccucaaacag agcacaauua ucgaauuuga guccucugga     5040

uuuaacaaga cgauuaaaga gaugcugggc augaagcucg cugggaucua caaugaaacc     5100

agcaacaaua agcgccccgc cgccaccaaa aaggcuggac aggccaaaaa gaaaaaguaa     5160

ggacuagucc cuguucccag agcccacuuu uuuuucuuuu uuugaaauaa aauagccugu     5220

cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5280

aaaaaaaaaa aaaaagaauu                                                 5300


<210>  4796
<211>  3572
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4796
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagcg gaacuacauu      240

cugggacucg acaucggcau uaccagcguu ggguauggaa ucauugauua cgagacacgc      300

gacgugaucg augcgggcgu gagacuguuc aaagaagcua augucgagaa caaugaaggg      360

cggcgcucca agagaggagc ccggcgccuu aagagacggc gcagacaucg gauccagcgc      420

gugaaaaagc ugcucuuuga cuauaaccug cuuacggauc acucugagcu gagcggcauu      480

aauccguacg aagccagagu uaaaggccuc ucccaaaagc ugucugagga ggaguucagc      540

gcugcccugc uccaucuggc uaaacggcgc ggggugcaca acgucaauga aguggaagag      600

gacaccggaa acgagcuuuc cacaaaggaa cagaucucua gaaauagcaa ggcccuggag      660

gaaaaauaug uugcggagcu ccagcuggaa cggcuuaaga aagauggcga ggugagaggg      720

uccauuaacc gcuuuaagac cagcgacuac gucaaagagg ccaagcagcu gcucaaagug      780

caaaaggcuu aucaucagcu ggaucagucu uucaucgaca cauacauuga ucugcucgaa      840

acccggagaa cauacuauga gggacccggc gaaggguccc cuuuuggaug gaaggacauc      900

aaagaguggu acgaaaugcu gaugggccac ugcaccuauu uccccgagga gcuucgcagc      960

gugaaguacg ccuauaaugc ugaucuguac aacgcccuca augaccugaa caaucuuguc     1020

auuacgcggg acgaaaacga gaaacuggaa uauuacgaga aguuucagau caucgaaaac     1080

guuuucaaac aaaagaagaa accuacccuc aagcagauug cgaaagagau ccuggugaau     1140

gaggaagaua uuaagggcua cagagugaca ucuaccggga aacccgaguu uacaaaccug     1200

aaggucuauc acgacaucaa ggauauuacc gcccgcaaag aaaucaucga gaaugcugag     1260

cuccuggacc agauugccaa gauccuuacg auuuaccagu ccagcgaaga uauccaagag     1320

gaacugacaa accucaauuc ugagcugacc caggaagaga uugagcagau cuccaaccuu     1380

aaaggauaua caggcaccca uaaucugagc cucaaggcua ucaaccugau ucuggacgaa     1440

cucuggcaca ccaaugauaa ccaaaucgcc auuuucaauc ggcugaaacu ugugccuaag     1500

aagguugacc ugucccagca gaaagagauc ccgacaacgc ucguggauga cuuuauucug     1560

ucucccgucg ugaagcgcag cuucauccag uccauuaaag uuaucaacgc caucauuaag     1620

aaauacgggc ugccuaauga uaucauuauc gaacuugcuc gcgagaagaa cagcaaagac     1680

gcgcaaaaga ugauuaauga aaugcagaag cggaacagac agaccaacga gcgcaucgag     1740

gaaaucauuc ggacaaccgg aaaagagaau gccaaguauc ucaucgagaa aauuaagcug     1800

caugacaugc aggaaggcaa augucuguac ucucucgaag cuaucccccu ggaggaucuu     1860

cugaacaauc cuuuuaacua ugagguggac cacauuaucc ccagauccgu cagcuucgau     1920

aauucuuuca acaauaaggu gcucgucaag caagaagaga acagcaaaaa ggggaaucgg     1980

acaccguuuc aguaccuguc cucuagcgac uccaaaauca gcuacgaaac cuucaagaaa     2040

cacauucuga accuugccaa gggaaagggc cgcaucucua aaacgaagaa agaguaucuc     2100

cuggaggaaa gagauauuaa ucgguuuucc gugcagaagg acuucaucaa cagaaaucug     2160

guugauacac gcuacgccac ccggggccuc augaaccugc uuagaagcua uuuucgcgug     2220

aauaaccugg acgucaaagu gaagucuauu aacgggggau ucaccuccuu ucucagacgg     2280

aaguggaaau ucaagaaaga gcgcaauaag ggcuacaaac aucacgcuga agaugcccug     2340

aucaucgcga acgcugacuu uauuuucaag gaguggaaaa agcuggauaa ggccaaaaag     2400

guuauggaaa aucagauguu ugaggagaaa caagccgaaa gcaugccuga gaucgaaaca     2460

gagcaggaau auaaggagau uuucaucaca ccccaucaga uuaaacacau caaggacuuu     2520

aaggauuaca aauauuccca uagaguggac aagaaaccua accgggagcu uauuaaugac     2580

acccucuacu cuacgcgcaa ggaugacaaa gggaacaccc ugaucgucaa uaaccugaau     2640

ggacucuacg auaaggacaa cgauaagcug aagaaacuua ucaauaaaag ccccgaaaag     2700

cugcucaugu aucaccacga cccccagaca uaccaaaaac ugaagcugau uauggagcag     2760

uauggcgaug aaaagaaccc ucuuuacaaa uauuacgagg aaaccgggaa uuaucucaca     2820

aaguacucca aaaaggacaa cggcccggug aucaaaaaga uuaaguacua uggaaauaaa     2880

cugaacgcuc aucuggauau caccgacgau uaccccaacu cuagaaauaa ggugguuaaa     2940

cucagccuga agccuuaucg guucgacguc uaccuugaua acggcgugua uaaauucgug     3000

acggucaaga aucuggacgu gauuaaaaag gagaacuacu augagguuaa uuccaagugc     3060

uacgaagagg ccaaaaagcu caaaaagauc agcaaccagg cugaauuuau cgccucuuuc     3120

uacaauaacg accugauuaa aaucaauggg gagcuguauc gcgugauugg agucaacaau     3180

gaucuucuga acagaaucga ggugaauaug auugacauca ccuaccggga auaucucgag     3240

aacaugaaug auaagagacc cccuagaauc auuaagacaa ucgcguccaa aacccaaagc     3300

auuaagaaau acucuacaga cauccugggc aaccucuaug aaguuaaguc caaaaagcac     3360

ccccagauua ucaagaaagg gaagcggccg gccgcuacca aaaaggccgg acaggcuaaa     3420

aagaagaaau aaggacuagu cccuguuccc agagcccacu uuuuuuucuu uuuuugaaau     3480

aaaauagccu gucuuucaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3572


<210>  4797
<211>  4640
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4797
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcccuguu caacaagugc      240

auuaucauua gcaucaaucu cgacuuuucc aacaaagaga aguguaugac caagccguac      300

ucuauuggac uggauaucgg cacaaauagc guuggguggg cggugaucac ggacaacuau      360

aaagugcccu ccaagaaaau gaagguccuu ggaaauaccu cuaaaaagua cauuaagaaa      420

aaccugcucg gcgugcugcu uuucgauagc gggaucacag cugaaggacg gcgccugaag      480

agaaccgccc ggcgcagaua uacacggcgc agaaaucgga uucucuaccu gcaggagauc      540

uuuuccaccg aaauggccac acuggacgau gcuuucuuuc aacgccucga cgauucuuuc      600

cugguuccug acgauaaaag agacagcaag uaucccauuu uuggcaaccu uguggaggaa      660

aaagucuacc augaugaguu cccuaccauc uaucaucugc ggaaguaccu cgccgacucc      720

acgaaaaagg cugaucugcg ccuuguguac cuggcccucg cgcacaugau uaaguauaga      780

ggccacuuuc ugaucgaggg ggaguucaau agcaaaaaca augacaucca gaagaacuuu      840

caggacuucc uggauaccua caaugccauu uuugaaucug accucucccu ggaaaacagc      900

aaacagcuug aggagaucgu uaaggauaaa auuucuaagc uggaaaagaa agaccggauc      960

cucaagcugu uccccggaga gaaaaauucc ggcauuuuca gcgaauuucu uaagcugauc     1020

guggggaacc aagcugauuu ccgcaaaugc uuuaaccucg acgagaaggc cucucugcau     1080

uucuccaagg aaagcuauga ugaggaccug gagacacucc ugggauacau cggcgaugac     1140

uauuccgaug ucuuucuuaa agcuaagaaa cuguacgacg ccauucuccu gucuggguuc     1200

cuuaccguga cagacaauga aaccgaggcg ccucugagcu ccgccaugau caagagauau     1260

aacgaacaca aagaggaucu cgcucugcug aaggaauaca uucggaauau cagccucaag     1320

acguacaacg agguguuuaa agacgauaca aagaauggau augccggcua cauugacggc     1380

aaaaccaacc aggaggauuu cuaugucuac cugaagaauc uucuggcuga auuugagggg     1440

gccgacuauu uccucgaaaa gaucgauaga gaggacuuuc ugcgcaagca gcggacauuc     1500

gauaacggau cuaucccgua ccagauucau cugcaagaaa ugagagccau ccuugacaaa     1560

caggcuaagu uuuaucccuu ccucgcgaag aauaaagagc gcauugagaa gauccugacc     1620

uuccggauuc cuuacuacgu uggcccccug gccagaggga acuccgauuu ugcuuggagc     1680

auccgcaaac ggaaugaaaa gauuaccccu uggaacuucg aggacgugau cgauaaagaa     1740

ucuagcgccg aggccuuuau caauagaaug acauccuucg accucuaucu gcccgaggaa     1800

aaggugcuuc cgaagcacuc ucugcucuac gagacguuua acgucuauaa ugaacugacc     1860

aaagugcgcu ucauugcuga gagcaugcgg gacuaccagu uucuggauuc caagcagaaa     1920

aaggacaucg uuagacuuua uuucaaagau aagcgcaagg ugacagacaa agauauuauc     1980

gaauaccucc acgccauuua uggauacgac ggcaucgagc ugaaggggau cgagaaacaa     2040

uuuaacagcu cucuguccac cuaccaugau cuccugaaca uuaucaauga caaggaguuc     2100

cuugaugaca gcucuaacga agcgauuauc gaagagauua uccacacacu gaccaucuuu     2160

gaagaucggg agaugauuaa acagagacuc uccaaguucg agaauaucuu ugacaagagc     2220

guccugaaaa agcugucccg gcgccacuau acgggauggg gcaaacuuuc ugcuaagcuc     2280

auuaacggca ucagagauga aaagagcggg aauacaauuc uggacuaccu gaucgacgau     2340

ggaaucucca accggaauuu caugcagcuc auucaugacg augcccuguc uuucaagaaa     2400

aagauccaaa aggcccagau uaucggcgac gaggauaaag ggaacauuaa ggaagugguu     2460

aaaagccuuc cuggaucccc cgcuaucaag aaaggcauuc ugcagagcau caagaucgug     2520

gacgagcucg ucaaggugau gggcgggaga aaaccugaau cuauugucgu ggagauggcc     2580

cgcgagaauc aguauaccaa ccaaggaaag uccaauagcc agcagcggcu gaaaagacug     2640

gaaaagucuc uuaaagagcu cggcuccaag auccugaagg aaaacauucc cgcuaaacug     2700

agcaagaucg auaauaacgc ccuccagaau gaccgccugu accuuuauua ccugcaaaac     2760

gggaaagaua uguauaccgg agacgaucuc gacauugaca gacugucuaa cuacgauauc     2820

gaccacauca uuccccaggc guuucugaag gauaauagca ucgacaacaa aguucuugug     2880

uccagcgccu cuaaucgggg caaguccgau gacuucccua gccuggaggu cgugaagaaa     2940

cgcaagacau uuugguacca gcuccugaaa uccaagcuca uuucucagag aaaauucgau     3000

aaccugacca aggcugagcg cgggggacuu cugccggaag acaaggccgg cuuuauccaa     3060

cggcagcucg uugagacaag acagauuacg aaacaugugg cucggcugcu ggaugaaaag     3120

uucaauaaca aaaaggacga gaauaaccgc gccgucagaa ccgugaaaau caucacacuu     3180

aagagcaccc ugguguccca guuucggaaa gauuucgaac ucuauaaggu ucgcgagauu     3240

aaugacuuuc accaugcgca cgaugccuac cugaacgcug ucaucgccuc ugcucuccug     3300

aagaaauauc ccaaacuuga gccugaguuc guguacgggg acuaucccaa auacaauagc     3360

uuuagagaac ggaaguccgc cacagaaaaa guguauuucu acucuaacau uaugaauauc     3420

uucaaaaaga gcauuucccu ggccgacggc agagucaucg agcgcccucu cauugaagug     3480

aacgaggaga ccggagaaag cguuuggaau aaagagucug aucuggcuac ggugcggaga     3540

guccuguccu acccccaagu gaacguugug aagaaagucg aggaacagaa ccacggccuu     3600

gaccgcggga agccgaaagg acuguuuaau gcgaaccuca gcucuaagcc uaagcccaau     3660

uccaacgaaa aucugguggg cgccaaagag uaucucgauc cuaagaaaua cgggggauau     3720

gcuggcauca gcaacucuuu cgccguucug gugaagggga ccaucgagaa aggcgccaag     3780

aagaaaauua caaauguccu ugaauuucag ggaaucucca uucuggaccg gaucaacuac     3840

agaaaggaua aacugaauuu ccuccuggag aagggcuaua aagacauuga acuuaucauc     3900

gagcugccca aguacagccu cuuugagcug uccgauggga gccgccggau gcucgcuucu     3960

auucugagca ccaacaauaa aagaggagaa auccauaagg gcaaccaaau uuuccuuucc     4020

cagaaguuug ugaaacugcu guaucacgcc aagcgcaucu cuaauacaau uaacgagaau     4080

caucggaaau acguggaaaa ccacaagaaa gaguucgagg aacucuuuua cuauauccug     4140

gaguucaaug aaaacuacgu cggggcgaag aagaacggaa aacuucugaa uagcgcuuuu     4200

caguccuggc agaaccacuc uaucgacgag cucuguagcu ccuucauugg cccuaccggg     4260

ucugaaagaa agggccuguu ugagcucacg agccggggau ccgccgccga uuucgaguuc     4320

cugggcguua aaaucccccg cuauagagac uacacaccga gcucucuucu gaaggaugcu     4380

acccugauuc aucaauccgu gacagggcuc uaugaaaccc ggaucgaccu ggccaaacuu     4440

ggagagggca agcgcccugc ggcuaccaag aaagccgggc aggccaagaa aaagaaauaa     4500

ggacuagucc cuguucccag agcccacuuu uuuuucuuuu uuugaaauaa aauagccugu     4560

cuuucagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4620

aaaaaaaaaa aaaaagaauu                                                 4640


<210>  4798
<211>  3716
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4798
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccuggua cgcgagccug      240

auguccgcuc aucaccuccg gguuggaauu gacgugggca cccauucugu ggggcuggcc      300

acacuucgcg ucgaugacca cggaacgccg aucgagcugc ucagcgcccu gucccauauu      360

cacgauucug gcguggggaa ggaagggaaa aaggaccacg auaccagaaa gaaacuuagc      420

ggcaucgcuc ggcgcgccag acggcugcuc caucaccgca gaacacagcu gcaacagcug      480

gacgagguuc uccgggaucu gggcuucccc auuccuaccc ccggggaauu ucuugaccug      540

aacgagcaga cagauccuua ucgcgugugg agaguccggg cucgccucgu ggaagagaag      600

cugcccgagg aacuuagagg accugccauc uccauggcgg uucggcauau cgcccgccac      660

agaggcuggc ggaauccgua cucuaaagug gagagccugc ucucccccgc ugaagagagc      720

ccuuucauga aggcccugag agagcgcauu cuggcuacca caggggaagu ccucgacgau      780

ggaaucaccc ccggccaggc cauggcgcaa guggcccuga cgcacaacau uucuaugcgg      840

gggccugagg gaauccuugg caaacugcau caguccgaca augcuaacga aauuagaaag      900

aucugcgccc gccagggcgu gagccccgau gucuguaagc agcuccugcg ggcuguuuuu      960

aaagccgacu cuccgagagg guccgccgug agccgcgugg cuccugaccc ccuuccugga     1020

caaggcucuu uccggagagc gcccaagugc gaucccgagu uucagcgcuu ccggauuauc     1080

uccaucgucg ccaaucugag aauuagcgaa accaaagggg agaaccgccc ucucacagcu     1140

gacgagcgga gacacguggu uaccuuucug acagaagauu cccaggccga ccugaccugg     1200

guggaugucg ccgagaagcu cggagugcau cggcgcgacc ugagaggcac ggcuguucac     1260

acagaugacg gggaacgguc ugccgcgaga ccgcccaucg augcuaccga ccgcauuaug     1320

cggcagacaa aaaucagcuc ccuuaagacc uggugggagg aagccgauag cgagcaaaga     1380

ggagccauga uucgcuaucu guacgaggac ccuaccgacu cugaaugugc ugagaucauc     1440

gccgaacucc ccgaggaaga ucaggcuaaa cuggacuccc uucaucugcc ugccggcaga     1500

gcggccuaua gccgggaguc ucucacagcu cugagcgauc acaugcuggc cacgaccgac     1560

gaucuccacg aggcucgcaa gagacuguuc ggcguggacg auuccugggc gcccccggcc     1620

gaagccauua augcuccugu cgggaacccc ucuguggacc ggacacuuaa gaucgucgga     1680

cgcuaccuga gcgccgugga guccaugugg ggcaccccug aaguuauuca uguggagcac     1740

gucagagaug gguuuacaag cgagcggaug gcugacgaac gcgauaaagc caauagacgg     1800

cgcuauaacg acaaucagga ggccaugaag aaaauccaaa gagauuacgg aaaggaaggc     1860

uacauuucuc ggggagacau cgugagacuc gacgcucugg agcugcaggg cugcgcgugu     1920

cuuuauugcg gaaccacgau cggcuaccau acaugucagc ucgaucacau uguuccccag     1980

gccgggccug gauccaacaa ucgccggggc aaccuggugg cugucugcga aagauguaau     2040

cgcagcaaau cuaacacccc cuucgccgug ugggcccaaa agugcgggau cccgcacgug     2100

ggaguuaagg aggcuauugg ccgggucaga ggguggcgca aacagacccc uaacacaucc     2160

agcgaggacc ugacccggcu caagaaagaa gugaucgcca gacugcgccg gacacaggag     2220

gaucccgaaa uugacgagag auccauggaa ucuguggcgu ggauggcuaa ugagcuucau     2280

caccggaucg ccgccgcuua ucccgagacg accgucaugg uguaccgcgg cagcauuaca     2340

gccgcggcua gaaaggccgc cggaaucgau ucccggauca accugauugg cgaaaaaggg     2400

cgcaaggaca gaaucgaucg gagacaucac gcuguugacg ccucuguggu cgcucucaug     2460

gaggccagcg uggcgaagac ccuggccgaa cgcuccagcc ugcggggaga gcagagacuu     2520

acaggcaaag aacaaaccug gaagcaguau acggggucua ccguuggagc ucgcgagcau     2580

uuugagaugu ggcggggcca caugcuccac cugacagaac uguucaauga gagacucgcc     2640

gaagauaaag uguacgucac ccagaacauu cgccugcggc uuuccgacgg gaaugcucau     2700

acagugaacc cuagcaagcu gguuucucac agacucggcg auggacugac cgugcagcaa     2760

aucgaccgcg ccuguacgcc ggcccugugg ugcgcucuua caagagagaa agauuuugac     2820

gagaagaaug gccuccccgc gcgggaagac cgcgccauua gaguccaugg gcacgagauc     2880

aaguccagcg auuauaucca gguguucucu aaacggaaga aaaccgacag cgaucgcgac     2940

gaaacaccuu ucggagcuau ugccgugaga ggcggguuug ucgagaucgg acccuccauu     3000

caucacgccc ggaucuacag aguugaaggc aagaaaccug uguacgcuau gcugcgcgug     3060

uucacccacg aucugcucag ccagcggcau ggggaccugu uuucugccgu cauucccccu     3120

caguccauca gcaugagaug cgcggagccc aaacuucgca aagcuaucac cacaggcaac     3180

gccacguauc ugggaugggu gguugugggc gaugagcucg aaauuaaugu cgacuccuuc     3240

accaaguacg ccaucgggcg guuucuggag gauuucccga acacaaccag auggcggauu     3300

uguggauaug acacaaauuc uaagcugacc cuuaaaccua ucgugcucgc ugccgaaggc     3360

cuggagaacc ccagcuccgc gguuaaugaa auuguggagc ugaaggggug gcgcgucgcu     3420

aucaacgugc ucacgaaagu ucacccuaca guggucagac gggaugcccu gggacgcccc     3480

agauacucua gccgguccaa ucuuccgacc ucuuggacca ucgagaagcg cccugccgcu     3540

acaaagaagg ccggccaagc uaagaaaaag aaauaaggac uagucccugu ucccagagcc     3600

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         3716


<210>  4799
<211>  4577
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4799
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaccaa gccguacagc      240

auuggacugg acaucggcac aaacuccguu gggugggcgg ugacgaccga uaauuauaaa      300

gugcccucua agaagaugaa aguccucgga aacacaagca agaaauacau uaagaaaaau      360

cugcuuggcg ugcuccuguu cgacuccggg aucaccgcug agggacggcg ccugaagaga      420

acagcccggc gcagauauac ccggcgcaga aaccggauuc uuuaccugca ggaaaucuuu      480

ucuacagaga uggccacccu cgaugacgcu uucuuucaac gccuggauga cagcuuccug      540

guuccugaug acaagagaga uuccaaauau cccaucuuug gcaaucucgu ggaagagaag      600

gccuaccaug acgaguuccc uacgauuuau caucugcgga aauaccuugc ugauucuacc      660

aagaaagccg accugcgccu cgucuaccug gcgcuugccc acaugaucaa guauagaggc      720

cacuuucuga uugaggggga guucaacagc aaaaauaacg auauccagaa gaauuuucag      780

gacuuccucg acacauacaa cgcuauuuuu gaauccgauc ugagccugga aaauucuaag      840

cagcucgaag agaucgugaa agacaagauu uccaaacugg agaagaaaga ucggauccuu      900

aagcuguucc ccggagaaaa gaacagcggc aucuuuucug aguuccucaa acugauuguu      960

gggaaucaag ccgacuuccg caagugcuuu aaccuugaug aaaaggcuuc ccugcauuuc     1020

agcaaagagu cuuaugacga agaucucgag acccugcugg gauacaucgg cgacgauuau     1080

uccgacgugu uucucaaagc caagaagcug uacgaugcga uucuucugag cggguuccuc     1140

acagucaccg acaaugagac ggaagccccu cuguccucug cuaugaucaa aagauauaac     1200

gagcacaagg aagaccuugc ccugcucaaa gaguacauuc ggaacaucag ccugaagaca     1260

uacaaugaag uguuuaaaga ugacaccaag aacggauaug cuggcuacau cgauggcaag     1320

acaaaucagg aggacuucua uguguaccug aaaaagcucc uggccgaguu ugaaggggcc     1380

gauuauuucc uugagaaaau ugacagagaa gauuuucugc gcaagcagcg gaccuucgac     1440

aacggaucca ucccguacca gauucaccuc caagagauga gagcuauccu ggauaaacag     1500

gcgaaguuuu aucccuuccu ggccaaaaau aaggaacgca uugagaagau ccuuaccuuu     1560

cggaucccuu acuacgucgg cccccucgcu agagggaaca gcgacuucgc cuggucuauu     1620

cgcaaacgga augagaagau cacaccuugg aacuucgaag auguuauuga caaagagucc     1680

agcgccgagg cuuuuaucaa uagaaugacg ucuuucgauc uguaucugcc cgaagagaag     1740

gugcucccga aacauagccu gcuuuacgaa accuuuaacg uguauaauga gcugacaaag     1800

guccgcuuca uugccgaauc caugcgggac uaccaguuuc ucgacucuaa gcagaaaaag     1860

gauaucguga gacuguauuu caaagacaag cgcaaaguua ccgauaagga cauuaucgag     1920

uaccugcacg cgaucuaugg auacgauggc auugaacuua aggggaucga gaaacaauuu     1980

aacagcuccc ucagcacaua ccaugaccug cugaauauua ucaacgauaa ggaguuccuc     2040

gacgauucuu ccaaugaagc uauuaucgag gaaaucauuc acacccugac gaucuuugag     2100

gaccgggaaa ugauuaaaca gagacuuagc aaguucgaga acaucuuuga uaaaucugug     2160

cugaagaagc ucucccggcg ccacuauaca ggauggggca aacugagcgc caagcugauu     2220

aacggcauca gagacgagaa auccgggaau accauccuug auuaccucau ugacgacgga     2280

aucucuaacc ggaauuucau gcagcugauu caugaugacg cccugagcuu uaagaaaaag     2340

auccaaaaag cucagauuau cggcgaugaa gacaagggga acaucaagga ggucgugaaa     2400

ucccucccug gaucucccgc cauuaagaaa ggcauccugc agagcauuaa gaucguugau     2460

gaacuuguga aagucauggg cgggagaaag ccugagucca uuguggucga aauggcucgc     2520

gagaaucagu auaccaacca aggaaagagc aauucucagc agcggcugaa aagacucgag     2580

aagucccuga aagaacuggg cagcaagauc cuuaaagaga acauucccgc caagcucucu     2640

aagaucgaca auaacgcgcu gcagaaugau cgccuguacc ucuauuaccu gcaaaacggg     2700

aaagacaugu auacaggaga ugaccuugau aucgacagac uguccaauua cgacauugau     2760

cacaucauuc cccaggccuu ccucaaggac aacagcaucg auaauaaagu gcugguuucu     2820

agcgcuucca accggggcaa gagcgacgau gugccuucuc uggaagucgu gaaaaagcgc     2880

aagaccuucu gguaccagcu ucugaaaucc aagcucauua gccagagaaa auuugacaac     2940

cugacaaagg ccgagcgcgg gggacucucc ccggaggaua aagcuggcuu cauccaacgg     3000

cagcugguug aaacgagaca gaucaccaag cauguggccc ggcuucugga cgagaaguuu     3060

aauaacaaaa aggaugaaaa uaaccgcgcg gucagaacag ugaaaauuau cacccucaag     3120

ucuacacugg ugagccaguu ccggaaagac uuugagcugu auaagguucg cgaaauuaau     3180

gauuuccacc augcccacga cgcuuaccuu aacgccgucg uggcuuccgc ccugcucaaa     3240

aaguauccca agcuggagcc ugaguuugug uacggggauu aucccaaaua caauucuuuc     3300

agagaaagaa agagcgccac cgagaaaguc uauuuuuacu ccaacaucau gaauauuuuc     3360

aagaaaucua ucagccucgc ugacggccgg gugaucgaac gcccucugau ugagguuaac     3420

gaagagacgg gagaguccgu guggaauaag gaaagcgacc uugcgaccgu ccggagagug     3480

cugucuuacc cccaaguuaa cguggucaag aaaguggagg aacagaauca cggccucgau     3540

cgcgggaagc cgaaaggacu guuuaacgcc aaucugucca gcaagccuaa acccaacucu     3600

aacgagaauc uuguuggcgc uaaggaauau cuggacccua agaaauacgg gggauaugcc     3660

ggcaucucca acagcuucac agugcucguc aaggggacca uugagaaagg cgccaagaaa     3720

aagaucacaa augugcugga guuccaggga auuucuaucc ucgaucggau uaacuacaga     3780

aaggacaaac ugaauuuucu ucuggaaaag ggcuauaaag auaucgagcu gaucauugaa     3840

cuccccaagu acucccuguu cgagcuuagc gacggguccc gccggaugcu ggcuagcauc     3900

cucucuacca acaauaaaag aggagagauu cauaagggca accaaaucuu ucugagccag     3960

aaauucguga agcuccugua ucacgccaag cgcauuucca auacgaucaa cgaaaaucau     4020

cggaaauacg ucgagaacca caagaaagaa uuugaggaac uuuucuacua uauccuggag     4080

uuuaaugaga acuacguugg ggcgaagaaa aauggaaagc ugcucaacuc ugcuuuccag     4140

agcuggcaga aucacuccau ugaugaacug uguucuagcu uuaucggccc uacagggucc     4200

gagagaaagg gccuuuucga acugaccucu cggggaagcg ccgccgacuu ugaguuccuc     4260

ggcgugaaaa uuccccgcua uagagauuac acaccgucca gccugcucaa ggacgcuacc     4320

cugauccauc aaucugugac cgggcuuuau gaaacacgga uugaucuggc caaacuggga     4380

gagggcaagc gcccugcggc uacgaaaaag gccgggcagg ccaagaaaaa gaaauaagga     4440

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4500

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aagaauu                                                    4577


<210>  4800
<211>  3659
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4800
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgcggc uuucaagccg      240

aacagcauua auuacauccu gggacucgac auuggcaucg ccuccguugg gugggccaug      300

guggagauug augaagagga aaaccccauc cggcugaucg accuuggagu gcgcgucuuu      360

gagagagcug aagugccuaa aaccggcgau ucucuggcca uggcucggcg ccucgccaga      420

agcguucggc gccugacaag acggcgcgcg cauagacuuc ugcggacgcg cagacuccug      480

aagcgggagg gggugcugca ggccgcuaau uucgacgaga acggacucau uaagucccug      540

cccaauaccc cuuggcaacu ucgcgccgcu gcccuggaua gaaaacucac accccuggaa      600

uggucugcgg uccuucugca ccucaucaag caucggggcu aucugagcca gcgcaaaaac      660

gagggcgaaa ccgccgacaa ggagcugggg gcucuccuga aaggaguggc cggcaaugcu      720

cacgcccuuc agacagggga uuuuagaacc ccugccgagc uggcucucaa caaguucgaa      780

aaggaguccg gacauauucg gaaucagaga ucugacuaca gccacacauu uucccgcaaa      840

gaucugcaag cggaacuuau ccuccuguuc gagaagcaga aagaauuugg caacccgcac      900

guuagcgggg gacugaagga gggcauugag acccugcuca ugacgcagcg gcccgcccug      960

ucuggcgacg cugugcagaa aaugcuuggg cauugcaccu ucgaaccugc cgagcccaag     1020

gccgcuaaaa auacauauac cgccgaaaga uuuaucuggc ugacaaagcu caacaaucug     1080

cgcauucuug agcaaggauc cgaacggccu cugaccgaua cggagagagc gacacucaug     1140

gacgagcccu accgcaagag caaacugacc uaugcucagg cccggaagcu gcucggccug     1200

gaagauacag ccuucuuuaa agggcuuaga uacggaaagg acaacgcuga ggccucuacc     1260

cugauggaaa ugaaggcuua ucacgccauc ucccgcgcgc ucgagaagga aggccugaag     1320

gacaaaaaga gcccgcugaa ucuuucuccu gagcuccagg augagaucgg gaccgccuuc     1380

ucccuguuua aaacagacga agauauuacg ggacggcuga aggacagaau ccagcccgag     1440

auucucgaag cucugcuuaa acauaucagc uucgauaagu ucguccaaau uucccugaag     1500

gcccuccggc gcaucgugcc ucugauggag cagggcaaaa gauacgacga ggcuugugcc     1560

gaaaucuacg gcgaucacua ugggaagaaa aacaccgagg aaaagauuua ccugccuccc     1620

auccccgcgg acgagauucg gaacccggug guccuuagag cccucucuca ggcucgcaaa     1680

guuaucaaug gaguggugcg gagauauggc agccccgccc gcauucacau cgaaacagcu     1740

agagaggucg ggaaguccuu uaaggaucgg aaagagaucg aaaagcgcca agaggaaaac     1800

agaaaagacc gggagaaggc cgccgcuaaa uuccgcgaau acuuuccuaa uuucguggga     1860

gagcccaaga gcaaagauau ucugaagcug agacucuaug agcagcagca uggcaagugc     1920

cuguacucug ggaaagaaau caaccuuggc cggcugaaug agaagggaua uguugaaauu     1980

gaccacgcgc ucccuuuuuc ccgcaccugg gacgauagcu ucaacaauaa agugcugguc     2040

cugggcucug agaaccagaa uaaggggaac caaacacccu acgaauacuu uaauggaaaa     2100

gacaacagca gagaguggca ggaguucaag gcccgggugg aaaccucccg cuuuccgaga     2160

ucuaagaaac agcggauccu ucuccagaag uucgaugagg acggcuuuaa agaaagaaau     2220

cugaacgaua cgcgcuaugu uaaucgguuc cugugucaau uuguggcuga cagaaugcgc     2280

cucacaggga agggcaaaaa gcgggucuuc gccagcaacg gacagauuac caaccugcuu     2340

agaggguucu ggggccugcg caaggugcgg gccgagaaug auagacauca cgcucucgac     2400

gccgucgugg uugcgugcuc caccguggcu augcagcaga aaaucacacg cuuuguccgg     2460

uacaaggaga ugaacgccuu cgauggaaaa accauugaca aggaaacagg cgaggugcug     2520

caucagaaaa cgcacuuucc ucagcccugg gaguucuuug cccaggaagu uaugaucaga     2580

guguucggga agccugaugg aaagcccgaa uuugaggagg cugacacccu ggaaaaacuu     2640

cggacacucc uggccgagaa gcugagcucu cgcccugaag cgguccacga guaugugaca     2700

ccccucuucg uguccagagc uccgaaucgg aaaaugagcg gccaggggca uauggaaacc     2760

guuaagucug ccaaacgccu ggaugaggga gucuccgugc uuagagugcc ucugacccaa     2820

cucaagcuga aagaccugga gaagaugguc aaccgggaaa gagagcccaa acuuuacgaa     2880

gcccugaaag cucgccucga ggcccacaag gacgaucccg cuaaagccuu ugcggaaccu     2940

uucuauaagu acgacaaagc cggcaaucgg acgcaacagg ugaaggcugu uagaguggag     3000

caaguccaga agaccggggu guggguucgc aaccauaaug gcaucgccga uaacgcuaca     3060

auggugcggg ucgacguguu ugagaaagga gauaaguauu accugguucc gauuuacagc     3120

uggcaagugg ccaaaggcau ccuccccgac agagccgucg ugcaggggaa ggaugaagag     3180

gacuggcagc ugauugauga cuccuucaau uuuaaauucu cucuucaccc uaacgaucug     3240

guggaaguca ucaccaagaa ggcucgcaug uucggauauu uugcgagcug ucaccggggc     3300

acagggaaua uuaacaucag aauccaugac cucgaucaca aaauuggaaa gaauggcauc     3360

cuggagggga uuggcguuaa aaccgcccug uccuuccaga aguaccagau cgacgagcuu     3420

ggaaaagaaa uucgccccug cagacugaag aagcggccuc ccgugcgcaa aagaccugcu     3480

gccacgaaga aagccggcca ggcuaagaaa aagaaguaag gacuaguccc uguucccaga     3540

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      3659


<210>  4801
<211>  4415
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4801
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagaa accguacacc      240

auuggacugg acaucggcac aaacagcguu gggugggcgg ugcucacgga ucaguaugac      300

cuggugaagc ggaagaugaa aauugcugga gauuccgaga agaaacaaau caagaaaaau      360

uucuggggcg uccgccuuuu ugacgaaggg cagaccgccg ccgauagacg gauggcucgc      420

acagccagac ggcgcauuga gagacggcgc aacagaaucu cuuaccugca gggaaucuuc      480

gcugaagaga ugagcaagac cgacgccaau uucuucugcc ggcucuccga uucuuuuuau      540

guggacaacg aaaagcgcaa uagcagacau cccuucuuug cgacaauuga ggaggaaguu      600

gaguaccaca aaaacuaucc uaccaucuac caucugcggg aggaacuugu caauuccucu      660

gagaaggccg aucugcgccu cgucuaucug gcucuggccc acauuaucaa auacagaggc      720

aacuuccuca uugaaggcgc ucuggacaca cagaauacca gcguggaugg gaucuacaag      780

caauuuauuc agacguauaa ccagguuuuc gccuccggaa ucgaggacgg cagccuuaaa      840

aagcuggaag auaauaaaga cguggcgaag auccucgucg agaaggugac ccggaaagaa      900

aagcuggagc gcauucuuaa acuguacccc ggggagaagu cugccggaau guuugcucag      960

uucaucuccc ucauuguggg cagcaaaggg aacuuucaaa agccuuucga ccugaucgaa     1020

aagucugaua uugagugugc caaagacucc uaugaagagg aucuggaaag ccuccuggcu     1080

cuuaucggag acgaguacgc cgagcuguuc gucgccgcua agaaugcgua uucugccguu     1140

gugcucucca gcaucauuac aguggcugaa accgagacaa acgccaaacu guccgccucu     1200

augaucgaaa gauuugauac ccaugaggaa gaccuuggcg agcugaaggc uuucauuaaa     1260

cuccaucugc ccaagcacua cgaggaaauc uuuagcaaua cggagaagca cggcuaugcc     1320

ggguacauug auggaaaaac aaagcaggcg gacuucuaca aauauaugaa gaugacccug     1380

gaaaacaucg agggcgcuga uuacuuuauc gccaaaauug agaaggaaaa cuuccuccgg     1440

aagcagagaa cauuugacaa uggggccauc ccucaccagc ugcaucuuga ggaacuggag     1500

gcuauucucc accaacaggc caaauauuac ccguuccuga aggaaaacua ugauaaaauc     1560

aagucccuug ucaccuuucg cauucccuac uucgugggac cucuggcuaa uggccagagc     1620

gaguuugccu ggcucacccg gaaagcggac ggggagauca gacccuggaa cauugaagag     1680

aagguugacu ucggaaaauc ugccguggau uuuaucgaaa agaugacaaa uaaggacacg     1740

uaucugccua aagagaacgu ccugcccaag cacucccucu gcuaccaaaa auaccuggug     1800

uauaaugaac uuaccaaggu ucgcuacauc aacgaucagg gcaaaacaag cuauuucucu     1860

ggccaggaga aggagcagau uuucaaugac cuguuuaagc aaaaacggaa ggugaaaaag     1920

aaagaucucg aacuguuccu gagaaacaug agccaugucg aguccccgac caucgaaggg     1980

cuugaggacu cuuuuaauag cuccuacagc acauaucacg aucuccugaa ggugggaauu     2040

aagcaggaaa uccuggacaa cccugucaau accgagaugc ucgagaacau ugugaaaauc     2100

cugacgguuu ucgaagauaa gcgcaugauc aaagagcagc uucagcaauu uucugacgug     2160

cuggauggcg ucgugcucaa gaaacuggaa cggagacauu acacagggug gggacgccug     2220

uccgcuaagc uccucauggg cauucgggac aagcagagcc accugaccau ccuggauuau     2280

cucaugaaug acgacgggcu gaacagaaac cuuaugcagc ugauuaauga uucuaaccuc     2340

uccuucaaaa gcaucauuga gaaggagcag guuaccacag ccgacaaaga uauccaaucc     2400

aucguggcug accuggccgg cucucccgcg auuaagaaag gaauccugca gagccuuaag     2460

auugucgaug aacucguguc cgugaugggc uacccucccc agaccaucgu ugucgagaug     2520

gcccgggaaa aucagacaac ggggaaagga aagaacaauu cucgccccag auacaagagc     2580

cuggagaaag cuauuaagga auuuggcucc caaauccuga aagagcaucc uaccgacaac     2640

caggagcucc ggaauaacag acuguaucuu uacuaucugc agaaugggaa ggauauguac     2700

acaggacaag accucgauau ccacaaccug agcaauuaug acauugauca caucgugccg     2760

cagucuuuca uuaccgacaa cuccaucgau aaucuggugc uuacaagcuc ugccggcaac     2820

cgcgaaaaag gggacgacgu gcccccucuc gagauugugc ggaagagaaa gguguuuugg     2880

gaaaaacugu accagggcaa ucugaugucc aagcgcaaau ucgauuaucu caccaaggcu     2940

gagagaggag gccugacgga agccgacaaa gcccgguuua uccaucgcca gcuuguugag     3000

accagacaaa uuacaaagaa cgucgcuaac auccugcacc agcgguucaa uuacgagaag     3060

gaugaccaug ggaacaccau gaaacaggug cgcaucguua cacucaagag cgcgcuggug     3120

ucucaguuua gaaaacaauu ccagcuguac aagguccggg augugaauga cuaucaccac     3180

gcccaugaug cuuaccuuaa cggaguugug gccaauaccc ugcucaaagu cuauccccag     3240

cuggaaccug aguucgugua cggcgacuau caccaguuug auugguucaa ggccaacaag     3300

gcuacggcca aaaagcaauu uuacacaaau auuaugcuuu ucuuugcgca gaaagaccgc     3360

aucauugaug aaaacgggga gauccugugg gacaagaaau aucuugacac cgugaagaag     3420

gucaugagcu acagacagau gaauauuguu aaaaagacag aaauccaaaa aggagaguuc     3480

uccaaggcua ccaucaaacc caagggcaac agcucuaaac ugauuccgcg gaagaccaau     3540

ugggauccua ugaaguacgg gggccucgac ucccccaaca uggccuaugc cguggugauc     3600

gaguacgcua aaggaaagaa uaaacugguc uuugaaaaga aaauuauccg cgugacaauu     3660

auggagagaa aggccuucga aaaggaugag aaagcguuuc uggaggaaca gggcuaucgg     3720

cagccuaagg uucuugcuaa acugcccaag uacacgcucu augaguguga agaggggaga     3780

cgccggaugc uggccagcgc caacgaagcu cagaaaggaa aucaacaggu gcucccuaac     3840

caucugguca cccuucugca ccaugccgcu aacugcgagg uguccgacgg caagucucuc     3900

gauuacaucg agagcaauag agaaauguuc gccgagcugc uggcgcacgu uuccgaauuu     3960

gccaagcgcu auacacuugc ugaggccaac cugaauaaaa ucaaccagcu cuuugaacag     4020

aauaaggagg gggacauuaa agcuaucgcc caaucuuucg uggaucugau ggccuuuaac     4080

gcuaugggag cgcccgccag cuucaaguuu uucgagacca caauugaacg gaaaagauac     4140

aauaaccuca aggagcugcu uaauuccacc aucauuuacc agucuaucac gggccuguau     4200

gaaagccgca agcggcugga cgauaaaaga ccggcugcca caaagaaagc cgggcaggcu     4260

aagaaaaaga aauaaggacu agucccuguu cccagagccc acuuuuuuuu cuuuuuuuga     4320

aauaaaauag ccugucuuuc agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                4415


<210>  4802
<211>  4448
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4802
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagaa accguacagc      240

auuggacugg acaucggcac caacuccguu gggugggcgg uggugacaga ugacuauaag      300

guccccgcua agaaaaugaa ggugcucgga aauacggaua aaucucauau ugagaagaac      360

cugcuuggcg cccuccuguu cgacagcggg aauaccgccg aagaucggcg ccugaaaaga      420

acagcucggc gcagauacac ccggcgcaga aaccggaucc uuuaucugca ggagauuuuc      480

uccgaagaga ugggaaaggu ugacgauucu uucuuucacc gccucgaaga cagcuuccug      540

gugacagagg auaagagagg cgagcggcau ccuaucuuug gcaaucugga agaggaaguc      600

aaauaccacg agaacuuccc caccaucuau caucuccgcc aauaccuggc cgacaauccu      660

gagaaggugg aucuuagacu gguuuaucuc gcucuggccc acauuaucaa auuucggggg      720

cacuuccuua uugaaggaaa guuugacaca cgcaacaaug augugcagag acuguuccag      780

gaguuucucg cggucuacga caacaccuuc gaaaauuccu cucugcagga gcaaaacguc      840

cagguggaag agauccugac ggacaaaauu agcaaguccg ccaaaaagga ucgggugcuc      900

aagcuguuuc ccaaugagaa aagcaacggc agauucgcug aauuucuuaa gcugaucguu      960

gggaaucagg ccgacuuuaa aaagcauuuc gagcucgaag agaaagcucc ucugcaguuu     1020

ucuaaggaua ccuacgaaga ggagcuugaa gugcugcucg cccaaauugg agacaacuau     1080

gcggagcugu uccuguccgc caagaaacuc uacgauagca uccugcuuuc uggcauccug     1140

acagugaccg acgucgggac aaaggcuccg cucuccgcca gcaugauuca gcgcuauaac     1200

gaacaccaga uggaucuggc ucagcuuaaa caauuuaucc ggcagaagcu gucugacaaa     1260

uacaaugagg uguucuccga uguuagcaag gacggauaug ccggcuacau ugauggcaag     1320

accaaccagg aagccuuuua uaaauaccuc aaggggcugc ugaauaaaau cgagggaucc     1380

ggcuacuucc ucgacaagau ugagagagaa gacuuucugc gcaaacaacg gacguucgau     1440

aacgggucua ucccccauca gauccaccuu caggagauga gagcuauuau ccgccggcag     1500

gcggaauuuu auccuuuccu ggccgacaau caagauagaa uugagaagcu ccugacauuu     1560

cgcauccccu acuauguggg accucuggcu cggggcaaga gcgacuucgc cuggcuuucc     1620

agaaaaagcg ccgauaagau uacccccugg aacuucgacg agaucgucga uaaagaaucu     1680

uccgcugagg ccuuuaucaa ucgcaugaca aacuacgacc ucuaucugcc gaaucagaag     1740

gugcugccua aacacagccu ccuguacgaa aaguucaccg uuuauaacga gcuuaccaaa     1800

gugaaguaca agacagaaca ggggaaaacg gcguuuuucg augcuaauau gaagcaggag     1860

auuuuugacg gagucuucaa aguguaccgg aaggucacca aagauaagcu gauggacuuu     1920

cucgagaagg aguucgauga auuuagaauc guggaccuga caggccugga caaagaaaac     1980

aagguuuuca augccucuua uggcaccuac caugaucuuu gcaaaauucu cgacaaggau     2040

uuucuggaca acagcaaaaa ugagaagauc cuggaagaua uugugcucac acugacccuu     2100

uucgaggacc gggagaugau ccgcaagaga cuggaaaacu auuccgaucu ccugacgaaa     2160

gagcaaguca agaaacugga acggagacac uacacagggu ggggacgccu uucugccgag     2220

cucauucaug gcauccggaa caaggaaagc agaaaaacca uccuggacua ucugauugau     2280

gacggaaauu ccaaccgcaa uuuuaugcag cucaucaacg augacgcucu gagcuucaag     2340

gaggagauug ccaaggcuca ggugaucggg gaaaccgaua aucuuaacca gguugugucu     2400

gacauugccg gcucccccgc gaucaaaaag gggauccugc aaagccucaa aauugucgac     2460

gagcugguga agaucauggg ccaccagccu gaaaauauug ugguugagau ggccagagag     2520

aaccaguuca caaaucaggg acggcgcaac ucucaacaga gacugaaagg ccuuaccgau     2580

uccaucaagg aauuugggag ccagauucuc aaagagcauc ccgucgaaaa uucccaacug     2640

cagaacgacc ggcuguuccu cuacuaucug cagaauggac gcgauaugua cacaggcgag     2700

gaacuugaca ucgauuaccu gucucaguau gacaucgauc acauuauccc ccaggcuuuu     2760

auuaaggaca acagcaucga uaauagagug cucacguccu cuaaggagaa ccgggggaaa     2820

agcgacgaug ugccuuccaa ggacgucgug cgcaaaauga aaagcuacug gucuaaacug     2880

cuguccgcca aacuuauuac ccaaagaaag uucgacaacc ugacaaaagc ugagcgggga     2940

ggccucaccg augacgauaa ggccggguuu aucaaacgcc agcugguuga aacaagacag     3000

aucaccaagc acguggcgcg gauucucgac gagagauuca auacggaaac cgaugagaac     3060

aauaaaaaga uccgccaagu caagauugug acacugaaaa gcaaccuugu uucuaauuuu     3120

cggaaggagu ucgaacugua uaaagugaga gagaucaacg acuaccauca cgcccaugau     3180

gcuuaucuca augccgucau uggcaaggcu cugcugggag uguacccgca gcuugaaccc     3240

gaguuuguuu auggcgacua cccucacuuc cacgggcaua aagaaaacaa ggccaccgcc     3300

aagaaauuuu ucuacuccaa uaucaugaac uucuuuaaga aagaugacgu gcgcacagau     3360

aagaauggag agauuaucug gaaaaaggac gaacacauca gcaacauuaa gaaaguccug     3420

ucuuaucccc aggugaauau cgugaagaaa gucgaggagc agaccggcgg guucagcaag     3480

gaauccauuc ucccuaaagg aaacagcgau aagcugaucc cccggaaaac gaagaaguuu     3540

uacugggaca caaagaagua uggcggguuc gacucuccga uuguugcuua cuccauccuc     3600

gugaucgcgg auauugagaa aggcaagagc aaaaagcuga agaccgugaa agcccuuguc     3660

ggagugacaa ucauggaaaa gaugaccuuu gagagagacc cuguugcuuu ccuggagcgc     3720

aaaggcuauc ggaaugugca agaagagaac auuaucaagc uccccaaaua cucccuguuu     3780

aagcuggaaa acgggagaaa gcgccuucug gccucugccc gggagcucca gaaaggaaau     3840

gaaauugucc ugccuaacca ucucggcacc cugcuuuauc acgcuaagaa uauccauaaa     3900

guggaugagc ccaagcaccu ggacuacguu gauaaacaca aggacgaguu caaggaacug     3960

cucgaugugg ucagcaacuu uuccaaaaag uacacacugg ccgaggggaa ucuugaaaag     4020

aucaaggagc uguaugcgca gaacaaugga gaagaccuca aagagcuggc uucuagcuuc     4080

auuaaccucc ugacguuuac cgccaucggc gccccugcua cauucaaguu uuucgauaaa     4140

aauauugaca gaaagcggua caccuccaca accgagaucc uuaacgccac gcugauucau     4200

caaucuauca cagggcugua ugaaacccgc auugaucuca auaagcuggg cggagacaaa     4260

agacccgcgg cuaccaagaa agccggccag gccaagaaaa agaaguaagg acuagucccu     4320

guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaagaauu                                                              4448


<210>  4803
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4803
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugcccagac aacaaaccug      240

agcuacauuc ucggacugga ccuuggcauc gcguccguug ggugggcugu gguggagauu      300

aaugaaaacg aggauccgau cggacugauu gacgucggcg ugcggaucuu cgaacgcgcc      360

gagguuccca agacggggga aucucucgcc cugagcagac ggcuugcucg cuccaccaga      420

cggcugaucc gcagacgggc ccaucgccuc cugcuggcua aaagauuucu caagcgggag      480

gggauucugu cuacaaucga ucuugagaag ggccugccua aucaagccug ggaacuccgc      540

guggcgggcc uggagagacg gcuuagcgcc auugaauggg gagcuguccu gcuccaccug      600

aucaaacauc gcggauaucu guccaagcgg aaaaacgagu cucagaccaa uaacaaggag      660

cucggcgccc ugcuuagcgg gguggcucag aaucaccagc ugcuccaauc cgacgauuac      720

agaacacccg ccgaacuggc gcuuaaaaag uucgccaagg aggaaggaca uauuagaaac      780

cagcgcggcg cuuauaccca cacauuuaau cggcuggacc uccuggccga gcugaaccuc      840

cuguucgcuc agcagcacca auuugggaau ccucauugca aagaacacau ccagcaguac      900

augaccgagc uucugaugug gcagaagccc gcccucagcg gagaggccau ucugaaaaug      960

cuuggcaagu guacgcauga aaagaacgag uucaaagcug cgaaacacac cuauucugcc     1020

gaaagauuug uuuggcugac aaagcucaau aaccugcgca uccuggagga uggcgcugaa     1080

cgggcccuca augaggagga aagacaacug cuuaucaacc acccuuacga gaaguccaaa     1140

cugaccuaug cccaggugcg caagcuccug gggcugagcg aacaggcuau uuucaaacau     1200

cuucgguacu cuaaggagaa cgccgaaucc gcgacauuua uggagcucaa ggcuuggcac     1260

gccaucagaa aagcccugga gaaucaagga cugaaggaca ccuggcagga ucucgcuaaa     1320

aagccggacc ugcuugauga aauuggcacg gccuucagcc uguacaaaac agacgaggau     1380

auccagcagu aucucaccaa caaggucccc aauucuguga uuaacgcucu gcuggugucc     1440

cuuaauuuug acaaauucau cgaacucagc cugaaguccc ugcgcaagau ccucccucug     1500

auggagcaag ggaaacggua cgaccaggcc ugcagagaga uuuauggaca ucacuacggc     1560

gaagcgaacc agaagacauc ucagcuucug cccgccaucc cugcucaaga gauucgcaau     1620

cccgucguuc uccggacccu gagccaggcc agaaaaguga ucaacgcuau uauccggcag     1680

uauggguccc cggcccgcgu gcauaucgaa accggaagag agcugggcaa gagcuuuaaa     1740

gaacggagag agauucagaa gcaacaggag gauaaucgca caaagcggga aucugcgguc     1800

cagaaauuca aggagcuuuu cuccgacuuu agcucugaac cuaaaagcaa ggauauccuc     1860

aaauucagac uguacgagca gcaacacggc aagugucugu auuccgggaa agaaauuaac     1920

auccaccgcc ucaaugagaa gggauacgug gagauugacc augcccugcc cuuuucuaga     1980

acgugggaug acagcuucaa caauaagguu cuugugcugg cuuccgaaaa ccagaauaaa     2040

ggcaaccaga ccccuuacga guggcuccaa gggaagauca acagcgaacg guggaaaaau     2100

uuugucgccc uggugcuggg aucucagugc uccgcugcca agaaacagcg ccuucucaca     2160

cagguuauug augacaacaa guucaucgau agaaaucuga acgacacccg guauaucgcc     2220

cgcuuucuga gcaauuacau ucaagagaac cuccugcuug ugggcaagaa uaaaaagaac     2280

gucuucacac ccaaugggca gaucaccgcu cugcucagau cucggugggg ccugauuaaa     2340

gcgcgcgaaa acaauaacag acaccaugcc cuggaugcua ucguggucgc cugugccacg     2400

cccuccaugc agcagaagau uacacgguuu auccgcuuca aagaggugca cccuuauaag     2460

aucgagaaua gauacgaaau gguugaccaa gagagcggag aaauuaucuc cccgcacuuu     2520

cccgagccuu gggcuuauuu ccggcaggag gugaacauua gagucuuuga caaucauccc     2580

gauaccgugc uuaaggaaau gcugccugac cgcccccagg ccaaccacca guucguucaa     2640

ccucucuucg ugucucgggc gccgaccaga aaaaugagcg gccaggggca uauggagaca     2700

aucaaguccg cuaaacgccu ggccgaagga auuucugucc uccggauccc ccugacccag     2760

cuuaagccua accugcucga gaauauggug aacaaagaaa gagagcccgc ccuguacgcu     2820

ggccugaagg cccgccuugc ggaguuuaau caagauccug cuaaggccuu cgccacaccc     2880

uuuuauaaac aagggggaca acaggugaag gcuauccggg uugaacaggu ccagaaaagc     2940

ggcgugcugg ugagagagaa caaugggguc gccgacaacg cuuccauugu gcgcacggau     3000

guuuucauca agaauaacaa auuuuuccuc gugccgauuu acaccuggca ggucgccaag     3060

ggcauccugc cuaauaaagc gauuguggcc cacaagaacg aagacgagug ggaagagaug     3120

gaugagggag cuaaguuuaa auucagccuc uuucccaaug accugguuga acuuaagacc     3180

aaaaaggagu acuucuuugg cuauuacauc gggcuggauc gggccacagg aaacaucucu     3240

cucaaagaac augacggcga gauuuccaag gggaaggaug gaguguauag agucggcgug     3300

aaacuggcuc ugagcuucga aaaguaccag guugacgagc uugggaaaaa ucggcagauc     3360

ugccgccccc agcagagaca gccugugcgg aagcgcccgg ccgccaccaa aaaggcuggc     3420

caagcgaaga aaaagaaaua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3480

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  4804
<211>  3659
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4804
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgcggc uuucaagccg      240

aaccccauua auuacauccu gggacucgac auuggcaucg ccagcguugg gugggccaug      300

guggagauug augaagacga gaacccuauc ugccugaucg aucuuggagu gcgggucuuu      360

gaacgcgcug aggugcccaa aaccggcgac ucccuggcca uggcuagacg gcucgcccgc      420

ucuguuagac ggcugacacg cagacgggcg caucgccuuc ugagagcccg gcgccuccug      480

aagagagaag gggugcugca ggcugccgau uucgacgaga auggacucau uaagagccug      540

ccuaacacgc ccuggcaacu ucgggcugcc gcgcuggauc gcaaacucac cccucuggag      600

ugguccgccg uccuucugca ccucaucaag cauagaggcu aucugucuca gcggaaaaau      660

gaaggcgaga cagcugacaa ggaacugggg gcccuccuga aaggaguggc ugauaacgcc      720

cacgcccuuc agaccggcga cuuuagaaca ccggcugagc uggcgcucaa uaaguucgag      780

aaggaaagcg ggcauauucg caaccagcgg ggagauuacu cccacaccuu uucuagaaaa      840

gaccugcaag ccgagcuuau ccuccuguuc gaaaagcaga aagaguuugg caauccccac      900

guuagcgggg gacugaagga aggcauugag acacugcuca ugacccagcg cccugcucug      960

uccggcgaug ccgugcagaa aaugcuuggg cauuguacgu ucgagcccgc cgaaccuaag     1020

gcugccaaaa acaccuauac agcggagcgg uuuaucuggc ugaccaagcu caauaaccug     1080

cgcauucuug aacaaggaag cgagagaccc cugacagaca ccgaacgggc uacgcucaug     1140

gacgagccgu acagaaaguc uaaacugaca uaugcccagg cccgcaagcu gcucggccug     1200

gaggauaccg cuuucuuuaa agggcuucgg uacggaaagg acaaugccga ggcuuccaca     1260

cugauggaaa ugaaagccua ucacgcgauc agcagagccc ucgaaaagga gggccugaag     1320

gauaaaaagu cuccucugaa ccuuuccccc gaacuccagg acgagaucgg gaccgcuuuc     1380

agccuguuua aaaccgauga ggacauuaca ggacgccuga aggaucggau ccagccugaa     1440

auucucgagg cccugcuuaa acauaucucu uucgacaagu ucguccaaau uucccugaag     1500

gcucucagac ggaucgugcc ccugauggaa cagggcaaac gcuacgauga ggccugcgcg     1560

gagaucuacg gcgaccacua ugggaagaaa aauacggaag agaagauuua ccugcccccu     1620

aucccggccg augaaauuag aaaccccgug guccuucggg cucucagcca ggccagaaaa     1680

guuaucaacg gaguggugcg ccgguauggc uccccugcuc gcauucacau cgagaccgcc     1740

agagaagucg ggaagucuuu uaaggacaga aaagagaucg agaagcggca agaagagaau     1800

cgcaaagaca gagaaaaggc cgcugcgaaa uuccgggagu acuuucccaa cuucguggga     1860

gaaccuaaga gcaaagauau ucugaagcug cgccucuaug agcagcagca uggcaagugu     1920

cuguacuccg ggaaagagau caaucuuggc agacugaacg aaaagggaua uguugagauu     1980

gaccacgccc uccccuuuag ccggacaugg gaugacucuu ucaauaacaa agugcugguc     2040

cugggcuccg aaaaucagaa caaggggaau caaaccccgu acgaguacuu uaacggaaaa     2100

gauaauagcc gcgaauggca ggaguucaag gcuagagugg agacaucucg guuuccucgc     2160

agcaagaaac agagaauccu ucuccagaag uucgacgaag auggcuuuaa agagcggaac     2220

cugaaugaca ccagauaugu uaaccgcuuc cugugccaau uuguggccga ucggaugaga     2280

cucacgggga agggcaaaaa gcgcgucuuc gccuccaaug gacagauuac aaaccugcuu     2340

cggggguucu ggggccugag aaaggugcgc gcugaaaacg accggcauca cgcccucgau     2400

gcggucgugg uugcuuguuc uaccguggcc augcagcaga aaaucaccag auuuguccgc     2460

uacaaggaga ugaaugccuu cgacggaaaa acaauugaua aggagaccgg cgaagugcug     2520

caucagaaaa cacacuuucc ccagccuugg gaguucuuug cucaggaagu uaugauccgg     2580

guguucggga agcccgacgg aaagccugag uuugaagagg ccgacacgcc cgagaaacug     2640

agaacccuuc ucgcggaaaa gcugagcucc cggccggagg cuguccacga auaugugaca     2700

ccucuguucg ugagccgcgc ccccaacaga aaaaugucug gccaggggca uauggagacc     2760

guuaaguccg ccaaacggcu cgaugaagga gucagcgugc ugcgcgugcc ccuuacacaa     2820

cugaagcuca aagaccugga gaagaugguc aauagagagc gggaaccuaa gcuguacgag     2880

gcucuuaaag ccagacugga agcucacaag gaugacccgg ccaaagcguu ugccgagccc     2940

uucuauaagu acgauaaagc uggcaaccgc acccaacagg ugaaggccgu ucggguggaa     3000

caaguccaga agacgggggu guggguuaga aaucauaacg gcaucgcuga caaugccacc     3060

auggugcgcg ucgauguguu ugagaaagga gacaaguauu accucguucc uauuuacucu     3120

uggcaagugg ccaaaggcau ccugcccgau cgggcugucg ugcaggggaa ggacgaggaa     3180

gauuggcagc ucauugacga uuccuucaac uuuaaauuca gccugcaccc uaaugaccuu     3240

guggagguca ucacaaagaa ggcgagaaug uucggauauu uugccuccug ccaccgcggc     3300

accgggaaca uuaauauccg gauccaugac cuggaucaca aaauuggaaa gaacggcauc     3360

cucgaaggga uuggcguuaa aacagcucug ucuuuccaga aguaccagau cgacgagcug     3420

ggaaaagaga uuagacccug ucgccuuaag aagagaccuc ccgugcggaa acgcccggcc     3480

gccaccaaga aagcuggcca ggccaagaaa aagaaguaag gacuaguccc uguucccaga     3540

gcccacuuuu uuuucuuuuu uugaaauaaa auagccuguc uuucagaucu aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaauu      3659


<210>  4805
<211>  3665
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4805
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaagua ccauguugga      240

auugacgugg gcaccuucag cguggggcug gcggcuaucg aggucgauga cgccggaaug      300

ccgauuaaaa cacucucccu ggugucucac auccaugaua gcggccuuga ccccgaugaa      360

auuaaguccg ccguuacgcg gcuggcuucu agcgggaucg cccgcagaac ccggcgccuc      420

uauagacgga agcgcagacg gcugcagcaa cuugacaaau uuauccagcg ccagggaugg      480

ccugugauug agcuggaaga uuacuccgac ccccucuauc cuuggaaggu cagagcugag      540

cuggccgcgu cuuacaucgc cgaugaaaaa gagcggggcg agaagcugag cguggcucuc      600

cgccacauug ccagacaucg gggcuggcgc aaccccuaug cuaaaguuuc cagccuguac      660

cuuccugacg ggccgucuga ugccuucaag gcgaucagag aagagauuaa gcgggccucc      720

ggacagcccg ugccugaaac agcuaccguc ggccaaaugg ugacacugug cgagcucggg      780

acccugaaac uuagaggaga gggcggggug cugagcgccc gccuccagca gucugacuau      840

gcucgggaaa uccaggagau uuguagaaug caagaaaucg gacaggagcu guaccgcaag      900

aucauugaug ucguuuuugc cgccgaaucc cccaaaggca gcgcuucuuc ccgggugggc      960

aaggacccuc ugcagcccgg gaaaaauaga gcgcucaagg ccagcgacgc uuuccagcgc     1020

uaccggaucg ccgcccugau uggaaaccuu agagugcgcg ucgauggcga gaaacggauc     1080

cuguccgugg aggaaaagaa ucucguuuuu gaccaccugg ugaaccuuac accgaagaaa     1140

gagccugaau gggucaccau ugcugagauc cuggggaucg auagaggaca acucauuggc     1200

acggccacca ugacagacga uggggaacgc gcgggagcuc ggcccccuac ccacgacaca     1260

aauagaucua ucgugaacag ccggauugcu ccccugguug auugguggaa gaccgccucc     1320

gcccuggagc agcaugccau ggugaaagcu cucagcaaug ccgaggucga cgauuucgac     1380

ucucccgaag gcgcgaaggu gcaggccuuu uucgcugauc uggacgacga uguccacgcc     1440

aaacuugacu cccugcaucu cccugugggc cgcgcugccu auagcgagga uacgcugguu     1500

agacuuacac ggagaaugcu gucugacggg guggaucucu acaccgcgcg ccugcaagaa     1560

uuuggaaucg agccgagcug gacacccccu acaccccgga uuggcgaacc ugucgggaac     1620

cccgccgugg acagaguucu gaagaccgug ucccgcuggc ucgagucugc uaccaagacg     1680

uggggagccc cggagagagu caucaucgaa cacgugcggg agggcuucgu gaccgaaaaa     1740

cgcgcuagag agauggaugg ggacaugcgg cgcagagccg cccggaaugc uaagcuguuu     1800

caggagaugc aggaaaaacu uaacguucag ggcaagccua gccgcgcgga ucuguggaga     1860

uaucaauccg uccagcggca gaauugccag ugugccuacu gcggaagccc cauuacauuc     1920

ucuaacuccg agauggacca caucgugccu cgcgcuggcc aagggagcac caauacaaga     1980

gaaaaccucg uggccgucug ucaucggugc aaucagucua aaggaaacac ccccuuugcc     2040

auuugggcua agaacacguc caucgagggc gugagcguua aggaagccgu ggagagaaca     2100

cgccacuggg ucaccgauac cgggaugcgg uccacagacu ucaaaaaguu caccaaagcg     2160

gugguugaga gauuucagcg cgcuacaaug gaugaagaga uugacgcccg gucuauggaa     2220

agcguggccu ggauggcuaa ugagcugaga ucccgcgucg cccagcauuu cgcgucucac     2280

ggaacgaccg ugcggguuua uagaggcagc cugacagcug aagcccgccg ggccuccggg     2340

aucagcggca agcugaaauu uuucgacgga gugggcaagu cuagacucga ucggcgccau     2400

cacgcuauug acgccgcugu caucgccuuu accuccgauu acguggcgga gacacuggcc     2460

gugagaagca accugaagca aucucaggcu caccggcagg aggccccuca guggcgcgaa     2520

uuuaccggga aagacgcuga gcauagagcc gccuggcggg ucugguguca aaagauggaa     2580

aaacucuccg cucugcuuac ggaggaucug agagacgauc gcguuguggu gaugagcaau     2640

guccggcuca gacugggaaa cggcucugcg cacaaggaaa ccaucgggaa acugagcaag     2700

gugaaacuuu ccagccagcu cucuguuucc gacauugaua aggccagcuc cgaggcucug     2760

uggugcgccc ugacacgcga gcccggauuu gacccgaagg aaggccuccc ugccaauccc     2820

gagcggcaua ucagagugaa cgggacccac gucuaugcug gcgauaauau uggacuguuc     2880

cccgugucug ccggcagcau cgcgcuucgc gggggauacg cugaacuggg cuccucuuuu     2940

caccaugccc ggguuuacaa aauuacaagc gggaagaaac cugccuucgc uaugcucaga     3000

guguauacca ucgaccugcu gccguaccgc aaccaggauc uuuucuccgu cgagcucaag     3060

ccccaaacga ugucuaugag acaggccgag aaaaagcugc gggacgcgcu ggcuacagga     3120

aaugccgaau aucucggcug gcuggugguu gacgaugagc uuguggucga caccagcaag     3180

aucgccacag aucaggugaa agcuguggaa gccgagcugg ggaccauucg cagauggcgg     3240

gucgacggcu ucuuuucccc uagcaagcuc cgccugagac cccugcagau gucuaaagaa     3300

ggaaucaaga aagaguccgc uccugagcuu agcaagauua ucgaucggcc cggcuggcuc     3360

ccugccguua acaagcuguu cucugacggg aaugugaccg uggucagacg cgauucccug     3420

ggacggguga gacucgaaag cacagcgcac cuucccguua cguggaaagu gcaaaagcgc     3480

ccggccgcua ccaaaaaggc cggccaggcu aaaaagaaga aauaaggacu agucccuguu     3540

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

gaauu                                                                 3665


<210>  4806
<211>  3365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4806
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccgcgcg gauucuggcu      240

uucgacaucg gaauuagcuc caucggcugg gccuuuucug agaacgauga acucaaggac      300

ugcgggguuc gcauuuucac caaaguggag aauccgaaga caggagaaag ccuggcccuu      360

cccagacggc uggcucgcuc cgccagaaag cggcucgcuc gcagaaaagc ccggcugaac      420

caucuuaagc accugaucgc gaaugaguuu aaacucaacu acgaagauua ucagucuuuc      480

gacgagagcc uggccaaggc uuacaaaggc ucccugaucu cuccuuauga gcuccgcuuu      540

agagcccuga augaacuucu gagcaagcaa gauuucgcuc gggugauucu ccauaucgcc      600

aagcgcagag gguacgacga uauuaaaaac uccgacgaua aggagaaagg agcgauccug      660

aaggccauua aacagaauga agagaaacuu gcuaacuauc agagcgucgg cgaguaccug      720

uauaaagaau acuuucagaa guucaaggaa aauucuaaag aauuuacgaa cgugcggaau      780

aagaaagagu ccuacgaacg cuguaucgcc cagagcuucc ucaaggacga gcugaaacug      840

auuuuuaaga agcaaagaga guucggcuuu ucuuucucca aaaaguucga agaggaaguu      900

cucagcgugg cuuuuuauaa acgggcccug aaggauuucu cucaccuugu cgggaacugc      960

uccuuuuuca ccgacgagaa aagagccccc aagaauagcc cucuggcuuu uauguucgug     1020

gcgcucacac gcaucaucaa ccugcuuaau aaccugaaga acaccgaagg cauucucuac     1080

acaaaagaug accugaaugc ccugcucaac gagguucuga agaauggaac ccuuacauau     1140

aaacagacca agaaacugcu cgggcugucc gacgauuacg aguuuaaggg agaaaagggc     1200

acguauuuca ucgaguuuaa aaaguacaaa gaguucauua aggcucuugg ggagcauaac     1260

cugucucagg acgaucucaa ugaaaucgcc aaagacauua cccugaucaa ggaugagauc     1320

aaacugaaga aggcccucgc uaaauaugac cugaaccaaa aucagauuga uagccuuucc     1380

aagcuggagu uuaaagacca ccucaacauc agcuucaagg cccugaaacu ggugacaccc     1440

cuuaugcucg aaggaaagaa guacgaugag gcguguaaug aacugaaccu gaaagucgcu     1500

auuaaugagg acaagaaaga uuuucucccu gccuucaacg agaccuacua uaaggacgaa     1560

gugacaaauc cggugguccu gcgggccauc aaagaguaca gaaagguucu uaacgcucug     1620

cucaagaaau auggcaaggu gcacaaaauu aauaucgaac uggcccgcga ggugggcaag     1680

aaccauucuc agcgggcuaa aaucgaaaag gagcagaacg agaauuacaa ggccaaaaag     1740

gacgcggaac uggagugcga aaaacuuggg cucaagauua acuccaaaaa uauccugaag     1800

cugagacucu ucaaagagca aaaggaauuu ugugccuaua gcggagagaa gauuaaaauc     1860

ucugaucugc aggacgagaa gaugcuugaa auugaucaca ucuaccccua uagccgcucc     1920

uucgacgauu cuuacaugaa caaaguccug guguuuacca agcagaauca agagaaacuc     1980

aaccagacgc cuuucgaggc uuuuggcaau gacagcgcca aguggcagaa gauugaaguu     2040

cuggcuaaaa accugcccac aaagaaacag aagcggaucc uugauaaaaa uuacaaggac     2100

aaggaacaaa agaacuucaa ggauagaaau cucaacgaca cccgcuauau cgcccggcug     2160

gugcugaauu acacaaaaga uuaucucgac uuucugccuc uuuccgauga cgagaacacc     2220

aagcugaaug acacccagaa agggagcaag guccaugugg aggcgaaguc uggaaugcuc     2280

acauccgccc ugagacacac guggggcuuc agcgcuaaag aucgcaacaa ccaucugcac     2340

cacgccauug acgcuguuau cauugccuac gccaauaacu cuaucgugaa ggcuuuuucc     2400

gauuucaaaa aggaacagga gagcaauucc gcggaacuuu augccaaaaa gauuucugag     2460

cucgacuaca agaacaaacg gaaguuuuuc gagcccuuua gcggguucag acagaaaguc     2520

cuggauaaga ucgacgaaau cuucgugucc aaaccggagc ggaagaaacc uucuggagcu     2580

cugcaugaag agaccuuucg caaggaagag gaguucuauc aaagcuacgg cggcaaggaa     2640

gggguccuca aagcccugga gcuuggaaag auuagaaaag ugaauggcaa gaucguuaaa     2700

aacggggaua uguuucgggu ggacauuuuc aagcacaaga aaacaaauaa guuuuacgcc     2760

guccccaucu auaccaugga uuucgcucug aaagugcucc cuaacaaggc cguugcgaga     2820

uccaaaaagg gagaaauuaa ggacuggauc cugauggaug agaauuacga auuuugcuuc     2880

agccuguaua aagacucucu uauccugauu cagacaaagg auaugcagga gcccgaguuu     2940

guguacuaua acgcuuucac cuccagcacg gucucucuca ucguguccaa acaugacaau     3000

aaguuugaaa cacugagcaa aaaccagaag auucucuuca agaaugccaa cgagaaagaa     3060

gugaucgcca agucuauugg cauccaaaau cugaaaguuu ucgagaagua caucgucagc     3120

gcucuugggg aagugaccaa agccgaguuu cgccagcggg aggacuucaa gaaaaagaga     3180

cccgcggcua ccaagaaagc cggccaggcc aagaaaaaga aauaaggacu agucccuguu     3240

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3300

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3360

gaauu                                                                 3365


<210>  4807
<211>  3776
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4807
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccagcga ccugguucuc      240

ggacuggaua uuggcaucgg guccguggga gugggcauuc uuaacaaggu caccggggag      300

aucauucaua aaaauucucg gaucuucccg gcggcucagg ccgaaaacaa ucuggugcgc      360

agaacaaacc ggcaaggacg cagacucgcc cggcgcaaga agcacagacg gguucgccug      420

aauagacuuu uugaggaaag cggccugauc acggacuuca ccaaaauuuc caucaaccuc      480

aaucccuacc agcugcgggu gaagggccug acagaugagc ucucuaacga ggagcuguuu      540

auugcucuua aaaauauggu caagcaucgc gggaucagcu aucuggacga ugccuccgac      600

gauggaaacu cuagcguggg cgacuacgcu cagauuguua aagaaaauuc caagcagcuc      660

gaaaccaaga caccugggca aauccagcug gagagauauc agaccuacgg acagcuucgg      720

ggcgauuuca caguggaaaa agacgggaag aaacaccgcc ugauuaacgu cuuucccacc      780

agcgccuaua gaucugaggc gcuccggauc cugcaaacgc agcaggaguu caauccucag      840

aucaccgaug aauuuauuaa cagauaccug gagauccuca caggaaagcg caaauauuac      900

cauggccccg gcaaugaaaa gucccggacc gacuacggga gauaucgcac aagcggagag      960

acccuggaua acauuuucgg cauccuuauu gggaaaugca cguuuuaccc ugacgaguuc     1020

cgggccgcua aggccucuua uacagcucaa gaauuuaacc ugcucaauga ccugaacaau     1080

cuuaccgugc cgacagagac caagaaacug uccaaggaac agaaaaacca gaucaucaau     1140

uacgugaaga acgagaaagc caugggaccc gcgaaacucu ucaaguauau ugccaaacug     1200

cugagcugug augucgcuga caucaagggc uacagaauug auaaaucugg gaaggccgaa     1260

auccacaccu uugaggcuua ucgcaaaaug aagacacucg aaacgcugga cauugagcaa     1320

auggaucggg agacccuuga caagcuggcc uacguucuca cacugaauac cgaaagagag     1380

ggaauccagg aagcccuuga gcacgaguuc gcugauggcu ccuucagcca gaaacaggug     1440

gacgaacugg ugcaauuucg caaggcgaac uccucuaucu ucggcaaagg guggcauaau     1500

uuuagcguca agcucaugau ggagcugauu ccugaacugu acgagacauc cgaggaacag     1560

augaccaucc ucacgcggcu gggaaaacag aagacaaccu cuucuuccaa caagaccaaa     1620

uauauugaug agaaacuucu gacagaagag aucuacaauc ccgugguugc caagagcgug     1680

agacaggcua uuaagaucgu caacgccgcc auuaaagaau auggcgacuu cgauaauauc     1740

gugaucgaga uggcucgcga aaccaacgag gacgacgaga agaaagccau ucaaaagauc     1800

cagaaggcga auaaagauga aaaggacgcu gccaugcuca aagccgcuaa ccaguacaac     1860

gggaaggccg agcugccuca cucuguuuuu cauggacaca aacagcuggc uacaaagauu     1920

cggcuuuggc accaacaggg cgaaagaugc cucuauacgg ggaagaccau cagcauucau     1980

gaucugauca auaacuccaa ucaguucgag guggaccaca uccugccccu cucuauuaca     2040

uuugaugaca gccuggccaa caaaguccuu guguacgcga ccgccaauca ggaaaagggc     2100

caacggacac cguaucaggc ucuggauucc auggacgaug ccuggagcuu ccgcgagcuc     2160

aaggcuuuug ucagagaguc uaagacccug uccaacaaaa agaaggaaua ccugcuuacg     2220

gaggaagaca ucagcaaauu cgaugugcgg aagaaauuua uugagagaaa ucucguugac     2280

acccgcuacg ccucucgggu gguccugaac gcgcugcagg aacauuucag agcccacaag     2340

aucgauacaa aaguguccgu ugugcgcgga caauuuacca gccagcucag acggcauugg     2400

ggcauugaga agacacgcga caccuaucac caccaugcug ucgacgcccu gaucaucgcu     2460

gccuccucuc agcuuaaucu guggaagaaa cagaagaaca cgcucgugag cuacuccgag     2520

gaucaacugc uggacauuga aacaggggag cuuaucucug augacgaaua uaaagagagc     2580

guguucaagg ccccuuacca gcacuucguu gauacccuca aauccaagga guuugaagac     2640

agcauucugu ucucuuauca ggucgauucc aaauuuaaua gaaagaucag cgacgcuaca     2700

auuuacgcga cccggcaggc caagguggga aaagauaagg cugacgagac cuaugugcug     2760

ggcaaaauca aggauaucua cacacaagac ggguacgaug ccuucaugaa aauuuauaag     2820

aaggacaaau cuaaguuucu cauguaccgc caugaccccc agacguucga aaaagucauc     2880

gagccuauuc uggaaaacua ucccaauaag cagaucaacg agaaaggaaa ggaggugccc     2940

uguaauccuu uucuuaagua caaagaggaa cacggcuaua uuagaaagua cuccaaaaag     3000

gggaacggcc cggaaaucaa aagccugaag uauuacgauu cuaagcucgg aaaccacauu     3060

gacaucaccc ccaaagauag caauaacaag guugugcugc aguccgucag cccuuggcgg     3120

gccgacgugu acuucaauaa aacaaccggc aaguaugaga uccuggggcu uaaauacgcu     3180

gaucuccaau uugaaaaggg aacaggcacc uauaagauuu cucaggagaa auacaacgac     3240

aucaagaaaa aggagggggu ugauuccgac agcgaguuca aauuuacgcu guauaagaau     3300

gaucugcucc uggugaaaga cacagaaacc aaggaacagc aacuuuuccg cuuucugucc     3360

agaaccaugc ccaagcagaa acauuacguc gagcucaagc cuuaugauaa acagaaguuc     3420

gaaggaggcg aggcccugau uaaagugcug gggaacguug cgaauagcgg ccagugcaag     3480

aagggacuug gcaaaagcaa caucuccauu uacaaggugc ggacagacgu ccuggggaau     3540

caacacauca ucaaaaacga gggagacaag cccaaacucg auuucaagcg cccggcugcc     3600

accaagaaag ccggccaggc uaagaaaaag aaauaaggac uagucccugu ucccagagcc     3660

cacuuuuuuu ucuuuuuuug aaauaaaaua gccugucuuu cagaucuaaa aaaaaaaaaa     3720

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         3776


<210>  4808
<211>  4334
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4808
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccaccca guucgaggga      240

uuuacaaacc uguaccaagu uagcaagacg cuccgguucg aacugauucc gcagggcaaa      300

acccuuaagc auauccagga gcagggguuu auugaagagg acaaggcgcg caaugaucac      360

uauaaagaac ugaagcccau cauugacaga aucuacaaaa cauaugcuga ucaaugccuc      420

cagcuggugc agcuugacug ggagaaccug uccgccgcca ucgauucuua ccggaaggag      480

aaaaccgaag agacacgcaa ugcucucauu gaagaacagg ccaccuauag aaacgcuauc      540

caugacuacu ucauuggacg gacagauaau cugaccgacg ccaucaacaa gcgccacgcg      600

gagauuuaua agggccuguu uaaagccgaa cucuucaaug ggaaggugcu gaaacaacuu      660

ggaacgguca ccacaaccga gcaugaaaac gcucugcuca gauccuuuga uaaguucaca      720

accuacuuua gcggcuucua cgagaaucgg aaaaacgugu uuucugccga agacaucagc      780

acggcuauuc cucaccgcau cguucaggau aauuucccca aguuuaaaga gaacugucac      840

aucuucacaa gacugauuac cgccgugccu ucccuucggg agcauuucga aaaugucaag      900

aaggcgaucg gcauuuuugu gucuacaagc aucgaggaag uuuucuccuu ucccuucuau      960

aaccagcugc ucacccagac ccaaauugac cuguacaauc agcugcucgg gggaaucagc     1020

cgcgaggccg gcacagagaa aaucaagggg cugaacgagg ugcuuaaccu ggcuauucag     1080

aaaaaugaug agacggccca caucauugcu ucucucccuc auagauuuau cccgcuguuc     1140

aagcaaauuc uuuccgaccg gaacacccug agcuuuaucc ucgaagaguu caaaucugac     1200

gaagagguca uccaguccuu uugcaaguau aagacacugc ugcgcaauga aaacgugcuc     1260

gagaccgccg aggcccuguu caaugaacuu aacagcauug aucugacaca caucuuuauu     1320

ucucacaaaa agcucgagac caucuccagc gcucugugug accauuggga uacgcuuaga     1380

aaugcgcugu acgaacggag aauuuccgag cucacaggaa aaaucaccaa gucugccaaa     1440

gagaaggugc agcgcagccu gaagcacgaa gacauuaacc ugcaggagau caucuccgcu     1500

gccggcaaag aacucagcga ggccuucaag cagaaaaccu cugaaauucu gucccaugcu     1560

cacgccgcgc uugaucaacc ccugccuaca acccucaaga aacaggagga gaaggaaauc     1620

cuuaagagcc agcuggacuc ucuucucggg cuguaucauc ugcucgauug guuugcuguc     1680

gacgagagca augaaguuga ucccgaguuc uccgcccggc ugacaggaau uaaacuugaa     1740

auggagccuu cucugagcuu cuacaacaag gccagaaauu augcuacgaa aaagcccuac     1800

uccguggaga aauuuaagcu caacuuccaa augccgaccc uggccagcgg cugggacgug     1860

aauaaagaaa agaacaaugg cgcuauccug uuugucaaga acgggcuuua cuaucucgga     1920

auuaugccua aacagaaggg ccgcuacaaa gcccugucuu ucgagcccac agaaaagacc     1980

uccgaggggu uugauaaaau guauuacgac uauuucccug acgcggccaa gaugaucccc     2040

aagugcagca cacagcugaa agcugugacc gcccacuuuc agacgcacac cacacccauc     2100

cuccugucua acaauuucau ugaaccucuu gagaucacca aggagauuua cgaucugaac     2160

aauccggaaa aagagcccaa gaaauuucaa acagcuuaug ccaagaagac cggagaccag     2220

aaaggcuacc gggaagcgcu cuguaagugg aucgauuuca cgagagacuu ucuguccaaa     2280

uacacaaaga ccacaagcau ugaucugucc ucucuucgcc cuagcuccca guauaaagac     2340

cucggggagu acuaugccga gcugaacccc cugcucuacc auaucucuuu ccagcggauc     2400

gcugaaaagg agauuaugga ugccguugaa accggaaagc uguaucuuuu ucaaaucuac     2460

aauaaagacu ucgcuaaggg ccaccauggc aaaccuaacc ugcacacccu cuauuggaca     2520

gggcuguuca gccccgagaa ucuggccaag acguccauua aacuuaacgg acaggccgaa     2580

cucuuuuaca gaccuaagag ccgcaugaaa cggauggcuc acagacuggg cgagaagaug     2640

cugaauaaga aacucaagga ucagaaaacc ccgauccccg acacacugua ccaagagcuu     2700

uaugauuacg ugaaccaucg ccugucucac gaccucuccg augaagcgcg ggcccugcug     2760

ccuaauguca uuaccaagga ggugagccau gaaaucauca aagacagacg guucacaucu     2820

gacaaguuuu ucuuucacgu ucccauuacc cuuaacuauc aggcugccaa uuccccuagc     2880

aaguucaacc agcgcgugaa ugccuaccug aaagagcauc ccgaaacgcc gaucauuggg     2940

aucgauagag gagagcggaa ccucauuuau aucacaguca uugacucuac cggcaagauc     3000

cuggagcaga gaagccucaa caccauccaa caguuugauu accagaaaaa gcuggacaau     3060

cgcgaaaaag agcggguggc ugccagacag gcgugguccg ucguggggac aauuaaggau     3120

cuuaagcaag gcuaucugag ccagguuauc cacgaaauug uggaccucau gauccacuac     3180

caggcugucg ugguucugga gaaccugaau uucggauuua aaucuaagcg caccggcauu     3240

gccgaaaaag ccguguacca gcaauucgag aagaugcuua ucgauaaacu gaacugccuc     3300

guccugaagg acuauccugc ugagaagguu gggggagugc ucaaucccua ccagcugaca     3360

gaucaguuua cguccuucgc caaaaugggc acccaaagcg gguuccuguu uuaugugccc     3420

gcgccuuaca cauccaagau cgacccgcug accggauucg ucgaucccuu uguguggaaa     3480

acaauuaaga accaugaauc uagaaaacac uuccucgagg gcuuugacuu ccugcauuau     3540

gaugugaaga ccggggacuu uauccugcac uucaagauga aucggaaccu uagcuuucag     3600

cgcggccugc cuggauucau gcccgcuugg gacauugucu uugagaaaaa ugaaacgcag     3660

uucgaugcca agggcacccc uuuuaucgcc gggaaaagaa uugugcccgu uaucgagaac     3720

caucgguuca caggacgcua cagagaccuc uauccugcua augaacugau cgcccuccug     3780

gaggaaaagg gcauuguguu ccgggauggg uccaacaucc uucccaaacu gcuggagaau     3840

gacgauucuc acgcuauuga caccaugguc gcccucaucc gcagcgugcu gcagaugaga     3900

aacuccaaug cggccacagg agaagauuac auuaacucuc cgguucggga ccuuaacggc     3960

guguguuuug auagccgcuu ccaaaauccu gaguggccca uggacgcuga ugccaacggg     4020

gcuuaccaca ucgcccugaa gggccagcuc cugcucaauc aucugaaaga guccaaggac     4080

cuuaagcugc agaacggaau uagcaaucag gacuggcugg ccuauaucca agaacucaga     4140

aacaaacggc cugcugcgac caagaaagcc ggccaggcua agaaaaagaa guaaggacua     4200

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     4260

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaag aauu                                                       4334


<210>  4809
<211>  4313
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4809
gggagagggc gcuuggcuug caaggacccu gagcugcggc auugaagcac acccaaccca       60

acucgacuga agucagccuc acugaaccgg aucugagaau cuucucucuc ugggcuugcc      120

agggcucucc gaaccuagcu agcauccucu ucaauuccaa cuagaaagcu uaccauggcc      180

cccaagaaaa agagaaaagu gggcauccac ggggucccug cugccagcau uuaccaggag      240

uucguuaaca aguauucccu gucuaaaacc cuccgguuug aacugauccc gcaaggaaag      300

acacuugaga auauuaaggc gcgcggccug auccucgacg augaaaagag agcuaaggac      360

uacaaaaagg ccaaacagau uaucgauaag uaucaucagu ucuuuaucga ggaaauucug      420

agcuccgugu gcaucucuga ggaccuucug cagaacuaca gcgaugugua uuucaagcuc      480

aaaaaguccg acgaugacaa ucugcaaaaa gauuuuaagu cugccaaaga cacgauuaag      540

aaacagauca gcgaguacau uaaggauucc gaaaaguuca aaaaccuguu uaaucagaac      600

cucaucgacg cuaagaaagg gcaggagagc gaucugauuc uuuggcugaa gcaaucuaaa      660

gacaauggaa ucgaacucuu caaggccaac uccgacauca ccgauauuga cgaggcucug      720

gagaucauua agagcuuuaa aggcuggaca accuauuuca agggguuuca cgaaaaucgg      780

aaaaacgucu acucuuccaa ugauaucccc acaagcauua ucuaccgcau cguggacgau      840

aaccuuccua aguuccugga gaauaaagcc aaguaugaau cucucaagga caaagcgccc      900

gaggccauua acuacgaaca gaucaagaaa gaucuggcug aggagcugac cuuugacauu      960

gauuauaaga cauccgaagu uaaucagaga guguucagcc ucgacgaggu cuucgaaauc     1020

gccaacuuua acaauuaccu gaaccagucc ggaauuacca aauucaauac gaucaucggc     1080

ggcaaguuug ugaacgggga gaauaccaag cggaaaggaa uuaacgaaua uaucaaucuu     1140

uacucucaac agauuaacga uaagacacug aaaaaguaua aaaugagcgu ucucuucaag     1200

cagauccugu ccgacaccga gagcaaaucu uuugugauug acaaacuuga ggaugacucc     1260

gaugucguga caaccaugca gagcuucuac gaacaaaucg cugccuuuaa gacgguggag     1320

gaaaaaucua uuaaggagac acugagccuc cuguucgacg aucugaaagc gcagaagcuc     1380

gaccugucca aaaucuacuu uaagaaugau aagucucuua ccgaccugag ccagcaaguc     1440

uucgaugacu auuccguuau cggcacagcc gugcucgaau acauuaccca gcagaucgcu     1500

ccuaaaaacc uggauaaucc cagcaagaaa gagcaggagc uuauugccaa gaaaaccgaa     1560

aaggcuaagu aucugucucu cgagacaauc aaacuggccc uggaagaguu uaacaagcau     1620

cgcgacauug auaaacaaug uagauucgag gaaauccucg ccaauuuugc ugcgaucccu     1680

augauuuucg acgagaucgc ccagaacaag gacaaucugg cucagauuuc caucaaauac     1740

cagaaccaag ggaagaagga ucuucugcag gccagcgccg aagacgaugu gaaagcuauu     1800

aaggaccucc uggaucagac gaacaaucug cuucacaaac ucaagaucuu ccauaucucu     1860

caguccgagg acaaagccaa cauucuggau aaggacgaac acuuuuaucu ggucuucgag     1920

gagugcuacu uugaacucgc gaauaucgug ccgcuguaua acaaaauucg gaauuacauc     1980

acccaaaagc ccuacagcga ugagaaguuc aaacuuaacu uugaaaauuc cacacuggcu     2040

aacggauggg acaagaauaa agagccugau aacaccgcca uucucuucau caaggacgau     2100

aaauauuacc ugggcguuau gaauaagaag aacaauaaaa ucuuugacga caaggccauu     2160

aaagaaaaca agggggaggg auauaaaaag aucguguaca agcugcuucc cggcgcuaau     2220

aaaaugcucc cuaaggucuu cuuuucugcc aaaagcauua aguucuauaa ccccuccgag     2280

gauauccugc gcauuagaaa ccacucuaca cauaccaaaa auggcagccc gcagaagggg     2340

uacgaaaagu uugaguucaa caucgaagac ugucggaaau uuauugauuu cuauaagcag     2400

uccaucagca aacacccuga guggaaggac uucggauuuc gcuucucuga uacgcaaaga     2460

uacaauucca ucgacgaauu uuaccgggag guggagaacc agggcuauaa acugacauuc     2520

gaaaauauua gcgagucuua caucgauucc guugugaacc aggggaagcu cuaucuguuu     2580

cagauuuaca auaaggacuu cagcgcuuau ucuaaaggac gccccaaccu ucauacccug     2640

uacuggaagg cccucuuuga ugaaagaaau cugcaagacg ucguguauaa acugaacggc     2700

gaggcggagc uuuucuaccg gaagcagagc aucccuaaaa agauuaccca ccccgccaaa     2760

gaagcuaucg ccaauaagaa caaggauaau cccaaaaagg aguccgucuu ugaauacgac     2820

cucaucaaag acaagagauu cacagaggau aaauuuuucu uccacugccc uauuaccauc     2880

aacuuuaaga gcucuggggc uaauaaguuc aacgacgaaa uuaaccugcu gcucaaagag     2940

aaggccaaug augugcauau ccuguccauu gaccgcggag agcggcaccu ugcguauuac     3000

acacugguug auggcaaagg caacaucauc aagcaggaca cguuuaauau uaucgggaac     3060

gauagaauga aaaccaauua ucaugacaag cucgccgcua uugaaaagga ucgcgacagc     3120

gcccggaaag auuggaagaa aaucaacaau auuaaggaga ugaaagaagg auaccugucc     3180

cagguggucc acgagaucgc uaagcuggug auugaauaua acgccaucgu uguguucgag     3240

gaccuuaauu uuggcuucaa gagagggcgc uuuaaagucg agaagcaagu guaccagaaa     3300

cucgaaaaga ugcugaucga gaaacugaac uaucucgugu ucaaggauaa ugaauuugac     3360

aaaacaggag gcguucugcg ggccuaccag cuuaccgcuc cguucgagac auuuaagaag     3420

auggggaaac agaccggcau uaucuacuau guccccgcgg gauucacguc uaagauuugu     3480

ccugugaccg gcuuugugaa ccaacuguac cccaaauaug aaagcgucuc caagucucag     3540

gaguucuuca gcaaauuuga caagaucugc uacaaucucg auaaggggua uuucgaguuu     3600

uccuucgacu acaaaaacuu uggagauaag gccgcuaaag gcaaguggac aauugccucu     3660

uucgggagca gacugaucaa uuuucgcaac uccgacaaaa aucauaacug ggauacccgg     3720

gaaguguauc cuacaaagga gcuggaaaaa cuucugaaag acuacagcau cgaguacgga     3780

cacggcgagu guauuaaggc cgcuaucugc ggggaaucug auaaaaaguu cuuugccaaa     3840

cucaccuccg uucugaacac gauucuccag augagaaaua gcaagacagg caccgagcug     3900

gacuaucuua ucucucccgu ggcggauguc aacggaaauu ucuuugacuc ccgccaagcu     3960

ccgaagaaca ugccucagga ugccgacgcc aauggcgcuu accacauugg gcugaaagga     4020

cucaugcugc ugggccggau caagaacaau caggaaggga aaaagcugaa ccuugugauc     4080

aaaaaugagg aauauuucga guuuguucag aacagaaaua acaagcggcc cgccgcgaca     4140

aagaaggcug gacaagccaa gaaaaagaaa uaaggacuag ucccuguucc cagagcccac     4200

uuuuuuuucu uuuuuugaaa uaaaauagcc ugucuuucag aucuaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4313


<210>  4810
<211>  4466
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4810
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccgacaag      180

aaauacagca uuggacugga uaucggcacc aacuccguug ggugggcggu gauuacagac      240

gaguauaagg ugccgucuaa gaaauucaag guccucggaa auacggaucg gcauagcauc      300

aaaaagaacc ugauuggcgc ucugcuguuu gacuccgggg aaaccgccga ggccacacgc      360

cucaaaagaa ccgcucggcg cagauacaca cggcgcaaga auagaaucug cuaucugcag      420

gaaaucuucu cuaacgagau ggccaaggug gaugacagcu uuuuccaccg gcuugaagag      480

uccuuucugg uugaggaaga uaaaaagcau gagcgccacc ccauuuucgg aaauaucgug      540

gacgaagucg cuuaccauga gaaauauccu accauuuacc accucagaaa gaaacuggug      600

gauucuacag acaaggccga ucugcggcuc aucuaucugg cgcuugccca caugauuaaa      660

uuucgcggcc auuuccugau cgagggcgac cucaaccccg auaauagcga cguugacaag      720

cuguuuauuc aacuugugca gaccuacaac cagcuguucg aagagaaucc uaucaacgcu      780

uccggggucg augccaaggc uauccucagc gccagacugu cuaaaucccg gcgccuggaa      840

aaucucauug cgcagcugcc cggagagaag aaaaacggcc uuuuugggaa ucugaucgcc      900

cucagccugg gacuuacgcc uaacuucaag ucuaauuuug accuggcuga agaugccaaa      960

cuccaacugu ccaaggacac cuacgaugac gaucuggaca accuccuggc ucagauuggc     1020

gaucaguaug ccgaccuuuu ccuggccgcu aagaaccuca gcgaugcgau ccugcuuucu     1080

gacauucuga gagugaauac agagaucacc aaagccccgc ucuccgcuag caugaucaag     1140

cgguacgacg agcaccauca ggaucugaca cugcucaaag cccuggugag acaacagcuu     1200

cccgaaaagu auaaagagau uuucuuugac caguccaaga acggguacgc cggauauauc     1260

gauggcggcg cuucucagga agaguucuac aaguuuauua aaccuauccu ggaaaagaug     1320

gacgggaccg aggagcuccu ggucaaacug aaucgcgaag aucuucuccg gaagcaaaga     1380

acguucgaca acggaagcau uccccaccag auccaccugg gcgagcugca ugccauccuc     1440

cgccggcagg aagauuuuua uccuuuccug aaagacaaua gagagaagau ugaaaagauc     1500

cuuacauuuc gcauucccua cuacguuggg ccgcuggcgc ggggaaacuc cagauucgcu     1560

uggaugaccc gcaaaagcga ggagacaauc accccuugga auuuugaaga ggugguggau     1620

aagggcgccu cugcccaauc cuucauugaa cggaugacca acuuugacaa aaaucucccc     1680

aacgagaagg uccugccuaa acacagccug cuuuaugagu acuucacagu guauaaugaa     1740

cucacgaagg uuaaauacgu gaccgagggg augagaaagc ccgcuuuucu gucuggagaa     1800

cagaagaaag ccaucgucga ucuucucuuc aagacaaacc gcaaagugac cguuaagcag     1860

cugaaagagg acuauuucaa gaagauugaa uguuuugaua gcguggagau cuccggcguc     1920

gaggaccggu ucaaugcuuc ucuuggcaca uaccaugacc ugcucaaaau cauuaaggau     1980

aaagacuuuc uggauaacga agagaaugaa gacauccugg aggauauugu gcuuacccuc     2040

acgcuguucg aagacagaga gaugaucgag gaacggcuga agacauaugc ccaccucuuu     2100

gaugacaaag ucaugaagca gcugaagcgc agacgguaca ccgggugggg aagacuuagc     2160

cgcaaacuga uuaacggcau ccgggauaag caauccggga aaaccauccu cgacuuccuu     2220

aagagcgaug gauuugcgaa cagaaauuuc augcagcuga uucaugacga uucucugaca     2280

uuuaaagagg acauccagaa ggcccaggug uccggccaag gggacagccu ccacgaacac     2340

auugcuaacc uggccggcuc ucccgcuauc aagaaaggca uucugcagac cguuaaggug     2400

gucgaugagc ucgugaaagu uaugggacgc cauaagccug aaaauaucgu gaucgagaug     2460

gccagagaga accagacaac gcagaaaggg caaaagaacu cccgggaacg caugaaaaga     2520

auugaggaag gaaucaagga gcugggcagc cagauucuua aggagcaccc ggucgaaaau     2580

acccagcugc agaaugagaa acucuaccug uauuaccugc aaaacgggcg ggacauguau     2640

guggaucagg aacuugacau caaucgccug uccgauuacg acguggauca uauuguuccc     2700

cagucuuucc ucaaggacga uagcaucgac aacaaagucc ugacaagauc cgauaagaau     2760

cggggaaaau cugacaacgu gccuagcgag gaagugguca agaagaugaa aaauuauugg     2820

cgccaacucc ugaacgcgaa acuuaucacc cagagaaaau uugacaaucu gacaaaggcc     2880

gagcggggcg ggcucuccga gcuggauaaa gcuggcuuca uuaagcgcca gcugguggaa     2940

accagacaga ucacgaagca cguugcccaa auucuugaca gccggaugaa caccaaauac     3000

gaugagaacg acaagcugau cagagaagug aaagucauua cacucaaguc uaaacuggug     3060

uccgauuuuc gcaaggacuu ccaguucuau aagguucggg agaucaauaa cuaccaccau     3120

gcucacgaug ccuaccucaa ugccgugguc ggaaccgcuc ugauuaaaaa guaucccaaa     3180

cuugaaagcg aguuugugua cggcgacuau aagguuuacg augugagaaa aaugaucgcg     3240

aagucugagc aggaaaucgg gaaggccaca gcuaaauauu ucuuuuacuc caacauuaug     3300

aauuucuuua agaccgagau cacgcuggcc aacggagaaa uucgcaaacg gccucucauc     3360

gagacaaaug gcgaaaccgg ggagauuguc ugggacaagg gaagagauuu cgccacagug     3420

cgcaaagugc ugagcaugcc ccaggucaac aucguuaaga aaaccgaggu gcaaaccggc     3480

ggguuuucua aggaaagcau ccugccgaag agaaauuccg acaaacuuau ugcucggaag     3540

aaagauuggg acccuaagaa auauggcgga uucgacagcc ccacaguggc cuacucuguc     3600

cuggugguug cgaaggugga gaagggcaaa uccaagaaac ucaagagcgu caaagaacug     3660

cucgggauca cgauuaugga gcgcuccucu uuugagaaga acccuaucga uuuccuggaa     3720

gcuaagggau acaaagaggu gaagaaagac cuuauuauca agcugcccaa auauagccug     3780

uuugaacucg agaauggccg gaagagaaug cuggccuccg ccggggaacu ucagaaggga     3840

aacgagcugg cucucccuuc uaaauacguu aauuuccugu aucucgccag ccauuacgag     3900

aagcugaaag gcucccccga agauaacgag cagaagcagc uuuucgugga acaacacaaa     3960

cauuaucugg acgagaucau ugaacagauc ucugaguuua gcaagcgggu cauucuggcg     4020

gaugcuaauc ucgacaaagu gcuguccgcc uacaacaagc accgcgauaa gccgaucaga     4080

gagcaggccg aaaacauuau ccaccuuuuc acccugacaa aucucggggc uccugccgcu     4140

uuuaaauauu ucgacaccac aauugaucgg aagcgcuaca ccagcacgaa agagguucug     4200

gacgccacac ucauccauca aucuaucacc ggccuguacg aaaccagaau ugaucuuucc     4260

cagcugggag gcgacaagcg gcccgcggcc acaaagaagg cugggcaggc caagaaaaag     4320

aaauaaggac uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua     4380

gccugucuuu cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa agaauu                                          4466


<210>  4811
<211>  5249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4811
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaacuuc      180

aagauucugc cgaucgcgau ugaccucgga guuaaaaaua caggcguguu uagcgcuuuc      240

uaccagaagg ggacaucccu ggagcggcuu gauaacaaga auggaaaagu guaugaacug      300

ucuaaggaca gcuacacgcu ccugaugaac aaucgcaccg ccagacggca ucaacgcaga      360

ggcaucgauc ggaaacagcu ugucaagcgc cuguuuaaac ucauuuggac agagcagcug      420

aaccuggaau gggacaagga uacccagcaa gccaucuccu uccucuuuaa uagacggggg      480

uucucuuuua ucacagacgg auauagcccc gaguaccuga acauugugcc ugaacagguu      540

aaggcuaucc uuauggauau uuucgacgau uauaauggcg aggacgaucu ggacuccuac      600

cucaaacugg ccaccgagca ggaaucuaag aucagcgaga uuuauaacaa acuuaugcag      660

aagauccugg aauuuaaacu caugaagcug ugcacagaua uuaaagacga caaggugucc      720

accaagacgc ugaaagagau caccagcuac gaguucgaac uccuggcuga uuaccuugcc      780

aauuauucug agucccugaa gacacaaaaa uuuagcuaca ccgacaagca gggcaaccuc      840

aaagaacugu cuuauuacca ccaugauaag uauaauaucc aggaguuccu uaagcgccac      900

gcgacaauua acgacagaau ccuggauacc cuccugacgg acgaucugga cauuuggaau      960

uuuaacuucg aaaaauucga uuuugacaag aacgaggaga aacuccagaa ucaagaagau     1020

aaggaccaua uccaggccca ucugcaccac uucgucuuug cugugaacaa aauuaagucc     1080

gagauggcca gcgggggacg gcaccgcucu caguacuucc aagaaaucac aaauguucuu     1140

gacgagaaca aucaucagga aggcuaucug aagaacuuuu gugagaaucu ccacaacaaa     1200

aaguacucca aucugagcgu gaaaaaccuu gucaaucuga ucgggaaccu cuccaaucug     1260

gagcugaagc cccucagaaa auacuucaac gauaagauuc acgcuaaggc cgaccauugg     1320

gaugaacaga aauuuaccga gacauauugc cacuggaucc ugggagaaug gcgggugggc     1380

gugaaggacc aggauaaaaa ggacggggcg aaauacucuu auaaggaucu uuguaaugag     1440

cugaaacaaa aggucaccaa ggccggacuc guugacuucc ugcuggaacu ugauccuugc     1500

cgcaccauuc ccccuuaccu cgacaacaac aauagaaaac cgcccaagug ucagagccug     1560

auccugaacc cuaaauuucu cgauaaucag uaucccaacu ggcagcaaua ccugcaggag     1620

cuuaagaaac ugcaguccau ucagaauuau cucgacagcu ucgagacaga ucugaaggug     1680

cugaagucuu ccaaagacca accuuacuuu guggaauaca agagcucuaa ccagcagauc     1740

gcuagcggcc agcgggacua uaaagaucuu gacgccagaa uccuccaauu cauuuuugau     1800

cgcgucaagg cuuccgacga gcugcugcuc aaugaaaucu acuuccaggc caaaaagcug     1860

aagcagaaag ccucuagcga gcuugagaag cuggaaucca gcaaaaagcu cgaugaggug     1920

auugcuaacu cucaacuguc ccagauccug aaaagccagc auacgaaugg cauuuucgaa     1980

caggggaccu uucuucaccu cguuugcaag uauuacaagc aacggcagag agcgcgcgac     2040

ucucggcugu auaucaugcc cgaguacaga uaugauaaaa agcugcauaa auacaacaau     2100

acaggacgcu ucgacgauga caaccagcuc cugaccuacu guaaucacaa gccgcggcag     2160

aaaagauauc aacuucugaa cgaucucgcc ggcgugcugc aggucucccc uaauuuucug     2220

aaggacaaaa uugggagcga ugacgaccuu uucaucucca aguggcucgu ggaacacauc     2280

cgcggauuua agaaggcuug cgaggauucu cugaaaauuc agaaagacaa ccggggccug     2340

cucaaccaua agaucaauau ugccagaaac acaaaaggga agugugagaa ggaaaucuuc     2400

aaucugauuu gcaaaaucga gggaagcgaa gauaagaaag gcaacuacaa gcacggccuu     2460

gccuaugagc uggggguucu ccuguuugga gaacccaaug aggcuuccaa accugaguuc     2520

gaccgcaaga ucaagaaauu uaacucuauu uacagcuucg cccagaucca acagauugcg     2580

uuugcugaac ggaagggcaa ugccaacacc ugugccgugu gcuccgcuga uaaugcccau     2640

agaaugcagc aaaucaaaau uacggagccc gucgaagaca acaaggauaa aaucauccug     2700

agcgcuaagg cccagcggcu ucccgcgauu ccuacacgca ucguggacgg ggccgucaag     2760

aaaauggcua ccauucuggc caagaauauc guggaugaca acuggcagaa uauuaaacag     2820

guucucucug cuaagcacca acugcacauc ccgaucauua ccgaguccaa cgccuucgaa     2880

uuugagcccg cgcucgccga ugugaaagga aagagccuga aggacagacg gaaaaaggcu     2940

cuugagagaa ucucuccuga aaauauuuuc aaagacaaga acaaccgcau caaagaguuc     3000

gccaagggca uuuccgcuua uagcggggcc aaucugacag auggcgacuu ugauggagcc     3060

aaagaggaac ucgaccauau cauuccccgg ucucacaaga aguacggcac ccugaacgau     3120

gaggcuaauc ugaucugcgu cacaagaggg gacaacaaaa auaagggaaa ccgcaucuuc     3180

ugucuuagag aucuggcgga caauuauaaa cucaagcagu uugaaacgac cgaugaccug     3240

gagauugaga aaaagaucgc cgauacaauu ugggacgcua acaagaaaga uuucaaguuu     3300

ggcaauuacc ggagcuucau caaccucacc ccucaggaac agaaagccuu ucgccaugcc     3360

cuguuccuug cugacgaaaa ucccauuaag caagccguga ucagagcgau caacaaucgg     3420

aaccgcacau uuguuaaugg gacccagaga uacuucgcug aggugcuggc caacaauauu     3480

uaucuccggg ccaaaaagga gaaccugaac acggacaaga ucuccuuuga uuacuucggc     3540

auuccgacca ucggaaaugg gcgcggcauu gcugaaauca gacagcugua ugagaaaguc     3600

gacagcgaua uccaggccua cgcgaaggga gacaaaccuc aggcuucuua uucccaccuu     3660

auugaugcca ugcuggccuu cugcaucgcu gccgacgaac aucggaacga uggcagcauu     3720

gggcucgaga ucgacaagaa uuacucccug uauccccucg auaaaaacac aggagaagug     3780

uuuaccaagg acauuuucuc ucaaaucaag auuacagaua augaguuuag cgacaaaaag     3840

cuggugcgca aaaaggcuau cgagggcuuc aacacccaca gacagaugac gcgggauggg     3900

aucuacgccg aaaauuaccu uccuauucug auccacaaag agcugaacga aguuagaaag     3960

ggauauacau ggaaaaauuc cgaggaaauu aagaucuuua agggcaaaaa guacgacauu     4020

cagcaacuca acaaucuggu cuauugucuu aaauucgugg acaagcccau cucuaucgau     4080

auucagauca gcacccugga ggagcuccgc aacauucuga caaccaauaa caucgcggcc     4140

accgcugaau acuauuacau uaaucucaaa acacagaagc ugcaugagua uuacaucgaa     4200

aacuacaaca cggcccuugg guauaagaaa uacuccaagg agauggaguu ucugcggucu     4260

cuggcuuaua gaagcgaacg cgugaaaauc aaguccauug acgaugucaa acaagugcuc     4320

gacaaggaua gcaauuucau cauuggcaag aucacccugc cuuuuaaaaa ggaguggcag     4380

cggcuuuaca gagaauggca gaacacaacc auuaaagacg auuaugaguu ccugaagucu     4440

uucuucaaug uuaaauccau cacaaagcuc cacaagaaag ugcgcaagga cuuuagccug     4500

cccauuucua ccaacgaagg aaaauuccuc gucaagcgga aaacguggga uaauaacuuc     4560

aucuaccaga uccugaauga cuccgauagc agagccgacg gcacaaagcc guuuauuccu     4620

gccuucgaca ucucuaaaaa cgagauugug gaggcuauca uugauuccuu uaccagcaag     4680

aauaucuucu ggcuucccaa gaacaucgaa cugcaaaaag uugacaauaa gaacauuuuu     4740

gcgaucgaua ccuccaaaug guucgaggug gaaacaccca gcgaccugcg cgauauuggg     4800

aucgccacca uucaguauaa gaucgacaau aacucucggc cuaaagucag agugaagcuc     4860

gauuacguua ucgacgauga cagcaagauu aauuacuuua ugaaccauuc ccugcuuaaa     4920

ucucgguauc cggauaaggu gcuggagauc cucaaacaga gcacaauuau cgaauuugag     4980

uccucuggau uuaacaagac gauuaaagag augcugggca ugaagcucgc ugggaucuac     5040

aaugaaacca gcaacaauaa gcgccccgcc gccaccaaaa aggcuggaca ggccaaaaag     5100

aaaaaguaag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     5160

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5220

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       5249


<210>  4812
<211>  3521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4812
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaagcgg      180

aacuacauuc ugggacucga caucggcauu accagcguug gguauggaau cauugauuac      240

gagacacgcg acgugaucga ugcgggcgug agacuguuca aagaagcuaa ugucgagaac      300

aaugaagggc ggcgcuccaa gagaggagcc cggcgccuua agagacggcg cagacaucgg      360

auccagcgcg ugaaaaagcu gcucuuugac uauaaccugc uuacggauca cucugagcug      420

agcggcauua auccguacga agccagaguu aaaggccucu cccaaaagcu gucugaggag      480

gaguucagcg cugcccugcu ccaucuggcu aaacggcgcg gggugcacaa cgucaaugaa      540

guggaagagg acaccggaaa cgagcuuucc acaaaggaac agaucucuag aaauagcaag      600

gcccuggagg aaaaauaugu ugcggagcuc cagcuggaac ggcuuaagaa agauggcgag      660

gugagagggu ccauuaaccg cuuuaagacc agcgacuacg ucaaagaggc caagcagcug      720

cucaaagugc aaaaggcuua ucaucagcug gaucagucuu ucaucgacac auacauugau      780

cugcucgaaa cccggagaac auacuaugag ggacccggcg aagggucccc uuuuggaugg      840

aaggacauca aagaguggua cgaaaugcug augggccacu gcaccuauuu ccccgaggag      900

cuucgcagcg ugaaguacgc cuauaaugcu gaucuguaca acgcccucaa ugaccugaac      960

aaucuuguca uuacgcggga cgaaaacgag aaacuggaau auuacgagaa guuucagauc     1020

aucgaaaacg uuuucaaaca aaagaagaaa ccuacccuca agcagauugc gaaagagauc     1080

cuggugaaug aggaagauau uaagggcuac agagugacau cuaccgggaa acccgaguuu     1140

acaaaccuga aggucuauca cgacaucaag gauauuaccg cccgcaaaga aaucaucgag     1200

aaugcugagc uccuggacca gauugccaag auccuuacga uuuaccaguc cagcgaagau     1260

auccaagagg aacugacaaa ccucaauucu gagcugaccc aggaagagau ugagcagauc     1320

uccaaccuua aaggauauac aggcacccau aaucugagcc ucaaggcuau caaccugauu     1380

cuggacgaac ucuggcacac caaugauaac caaaucgcca uuuucaaucg gcugaaacuu     1440

gugccuaaga agguugaccu gucccagcag aaagagaucc cgacaacgcu cguggaugac     1500

uuuauucugu cucccgucgu gaagcgcagc uucauccagu ccauuaaagu uaucaacgcc     1560

aucauuaaga aauacgggcu gccuaaugau aucauuaucg aacuugcucg cgagaagaac     1620

agcaaagacg cgcaaaagau gauuaaugaa augcagaagc ggaacagaca gaccaacgag     1680

cgcaucgagg aaaucauucg gacaaccgga aaagagaaug ccaaguaucu caucgagaaa     1740

auuaagcugc augacaugca ggaaggcaaa ugucuguacu cucucgaagc uaucccccug     1800

gaggaucuuc ugaacaaucc uuuuaacuau gagguggacc acauuauccc cagauccguc     1860

agcuucgaua auucuuucaa caauaaggug cucgucaagc aagaagagaa cagcaaaaag     1920

gggaaucgga caccguuuca guaccugucc ucuagcgacu ccaaaaucag cuacgaaacc     1980

uucaagaaac acauucugaa ccuugccaag ggaaagggcc gcaucucuaa aacgaagaaa     2040

gaguaucucc uggaggaaag agauauuaau cgguuuuccg ugcagaagga cuucaucaac     2100

agaaaucugg uugauacacg cuacgccacc cggggccuca ugaaccugcu uagaagcuau     2160

uuucgcguga auaaccugga cgucaaagug aagucuauua acgggggauu caccuccuuu     2220

cucagacgga aguggaaauu caagaaagag cgcaauaagg gcuacaaaca ucacgcugaa     2280

gaugcccuga ucaucgcgaa cgcugacuuu auuuucaagg aguggaaaaa gcuggauaag     2340

gccaaaaagg uuauggaaaa ucagauguuu gaggagaaac aagccgaaag caugccugag     2400

aucgaaacag agcaggaaua uaaggagauu uucaucacac cccaucagau uaaacacauc     2460

aaggacuuua aggauuacaa auauucccau agaguggaca agaaaccuaa ccgggagcuu     2520

auuaaugaca cccucuacuc uacgcgcaag gaugacaaag ggaacacccu gaucgucaau     2580

aaccugaaug gacucuacga uaaggacaac gauaagcuga agaaacuuau caauaaaagc     2640

cccgaaaagc ugcucaugua ucaccacgac ccccagacau accaaaaacu gaagcugauu     2700

auggagcagu auggcgauga aaagaacccu cuuuacaaau auuacgagga aaccgggaau     2760

uaucucacaa aguacuccaa aaaggacaac ggcccgguga ucaaaaagau uaaguacuau     2820

ggaaauaaac ugaacgcuca ucuggauauc accgacgauu accccaacuc uagaaauaag     2880

gugguuaaac ucagccugaa gccuuaucgg uucgacgucu accuugauaa cggcguguau     2940

aaauucguga cggucaagaa ucuggacgug auuaaaaagg agaacuacua ugagguuaau     3000

uccaagugcu acgaagaggc caaaaagcuc aaaaagauca gcaaccaggc ugaauuuauc     3060

gccucuuucu acaauaacga ccugauuaaa aucaaugggg agcuguaucg cgugauugga     3120

gucaacaaug aucuucugaa cagaaucgag gugaauauga uugacaucac cuaccgggaa     3180

uaucucgaga acaugaauga uaagagaccc ccuagaauca uuaagacaau cgcguccaaa     3240

acccaaagca uuaagaaaua cucuacagac auccugggca accucuauga aguuaagucc     3300

aaaaagcacc cccagauuau caagaaaggg aagcggccgg ccgcuaccaa aaaggccgga     3360

caggcuaaaa agaagaaaua aggacuaguc ccuguuccca gagcccacuu uuuuuucuuu     3420

uuuugaaaua aaauagccug ucuuucagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3521


<210>  4813
<211>  4589
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4813
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcccuguuc      180

aacaagugca uuaucauuag caucaaucuc gacuuuucca acaaagagaa guguaugacc      240

aagccguacu cuauuggacu ggauaucggc acaaauagcg uugggugggc ggugaucacg      300

gacaacuaua aagugcccuc caagaaaaug aagguccuug gaaauaccuc uaaaaaguac      360

auuaagaaaa accugcucgg cgugcugcuu uucgauagcg ggaucacagc ugaaggacgg      420

cgccugaaga gaaccgcccg gcgcagauau acacggcgca gaaaucggau ucucuaccug      480

caggagaucu uuuccaccga aauggccaca cuggacgaug cuuucuuuca acgccucgac      540

gauucuuucc ugguuccuga cgauaaaaga gacagcaagu aucccauuuu uggcaaccuu      600

guggaggaaa aagucuacca ugaugaguuc ccuaccaucu aucaucugcg gaaguaccuc      660

gccgacucca cgaaaaaggc ugaucugcgc cuuguguacc uggcccucgc gcacaugauu      720

aaguauagag gccacuuucu gaucgagggg gaguucaaua gcaaaaacaa ugacauccag      780

aagaacuuuc aggacuuccu ggauaccuac aaugccauuu uugaaucuga ccucucccug      840

gaaaacagca aacagcuuga ggagaucguu aaggauaaaa uuucuaagcu ggaaaagaaa      900

gaccggaucc ucaagcuguu ccccggagag aaaaauuccg gcauuuucag cgaauuucuu      960

aagcugaucg uggggaacca agcugauuuc cgcaaaugcu uuaaccucga cgagaaggcc     1020

ucucugcauu ucuccaagga aagcuaugau gaggaccugg agacacuccu gggauacauc     1080

ggcgaugacu auuccgaugu cuuucuuaaa gcuaagaaac uguacgacgc cauucuccug     1140

ucuggguucc uuaccgugac agacaaugaa accgaggcgc cucugagcuc cgccaugauc     1200

aagagauaua acgaacacaa agaggaucuc gcucugcuga aggaauacau ucggaauauc     1260

agccucaaga cguacaacga gguguuuaaa gacgauacaa agaauggaua ugccggcuac     1320

auugacggca aaaccaacca ggaggauuuc uaugucuacc ugaagaaucu ucuggcugaa     1380

uuugaggggg ccgacuauuu ccucgaaaag aucgauagag aggacuuucu gcgcaagcag     1440

cggacauucg auaacggauc uaucccguac cagauucauc ugcaagaaau gagagccauc     1500

cuugacaaac aggcuaaguu uuaucccuuc cucgcgaaga auaaagagcg cauugagaag     1560

auccugaccu uccggauucc uuacuacguu ggcccccugg ccagagggaa cuccgauuuu     1620

gcuuggagca uccgcaaacg gaaugaaaag auuaccccuu ggaacuucga ggacgugauc     1680

gauaaagaau cuagcgccga ggccuuuauc aauagaauga cauccuucga ccucuaucug     1740

cccgaggaaa aggugcuucc gaagcacucu cugcucuacg agacguuuaa cgucuauaau     1800

gaacugacca aagugcgcuu cauugcugag agcaugcggg acuaccaguu ucuggauucc     1860

aagcagaaaa aggacaucgu uagacuuuau uucaaagaua agcgcaaggu gacagacaaa     1920

gauauuaucg aauaccucca cgccauuuau ggauacgacg gcaucgagcu gaaggggauc     1980

gagaaacaau uuaacagcuc ucuguccacc uaccaugauc uccugaacau uaucaaugac     2040

aaggaguucc uugaugacag cucuaacgaa gcgauuaucg aagagauuau ccacacacug     2100

accaucuuug aagaucggga gaugauuaaa cagagacucu ccaaguucga gaauaucuuu     2160

gacaagagcg uccugaaaaa gcugucccgg cgccacuaua cgggaugggg caaacuuucu     2220

gcuaagcuca uuaacggcau cagagaugaa aagagcggga auacaauucu ggacuaccug     2280

aucgacgaug gaaucuccaa ccggaauuuc augcagcuca uucaugacga ugcccugucu     2340

uucaagaaaa agauccaaaa ggcccagauu aucggcgacg aggauaaagg gaacauuaag     2400

gaagugguua aaagccuucc uggauccccc gcuaucaaga aaggcauucu gcagagcauc     2460

aagaucgugg acgagcucgu caaggugaug ggcgggagaa aaccugaauc uauugucgug     2520

gagauggccc gcgagaauca guauaccaac caaggaaagu ccaauagcca gcagcggcug     2580

aaaagacugg aaaagucucu uaaagagcuc ggcuccaaga uccugaagga aaacauuccc     2640

gcuaaacuga gcaagaucga uaauaacgcc cuccagaaug accgccugua ccuuuauuac     2700

cugcaaaacg ggaaagauau guauaccgga gacgaucucg acauugacag acugucuaac     2760

uacgauaucg accacaucau uccccaggcg uuucugaagg auaauagcau cgacaacaaa     2820

guucuugugu ccagcgccuc uaaucggggc aaguccgaug acuucccuag ccuggagguc     2880

gugaagaaac gcaagacauu uugguaccag cuccugaaau ccaagcucau uucucagaga     2940

aaauucgaua accugaccaa ggcugagcgc gggggacuuc ugccggaaga caaggccggc     3000

uuuauccaac ggcagcucgu ugagacaaga cagauuacga aacauguggc ucggcugcug     3060

gaugaaaagu ucaauaacaa aaaggacgag aauaaccgcg ccgucagaac cgugaaaauc     3120

aucacacuua agagcacccu ggugucccag uuucggaaag auuucgaacu cuauaagguu     3180

cgcgagauua augacuuuca ccaugcgcac gaugccuacc ugaacgcugu caucgccucu     3240

gcucuccuga agaaauaucc caaacuugag ccugaguucg uguacgggga cuaucccaaa     3300

uacaauagcu uuagagaacg gaaguccgcc acagaaaaag uguauuucua cucuaacauu     3360

augaauaucu ucaaaaagag cauuucccug gccgacggca gagucaucga gcgcccucuc     3420

auugaaguga acgaggagac cggagaaagc guuuggaaua aagagucuga ucuggcuacg     3480

gugcggagag uccuguccua cccccaagug aacguuguga agaaagucga ggaacagaac     3540

cacggccuug accgcgggaa gccgaaagga cuguuuaaug cgaaccucag cucuaagccu     3600

aagcccaauu ccaacgaaaa ucuggugggc gccaaagagu aucucgaucc uaagaaauac     3660

gggggauaug cuggcaucag caacucuuuc gccguucugg ugaaggggac caucgagaaa     3720

ggcgccaaga agaaaauuac aaauguccuu gaauuucagg gaaucuccau ucuggaccgg     3780

aucaacuaca gaaaggauaa acugaauuuc cuccuggaga agggcuauaa agacauugaa     3840

cuuaucaucg agcugcccaa guacagccuc uuugagcugu ccgaugggag ccgccggaug     3900

cucgcuucua uucugagcac caacaauaaa agaggagaaa uccauaaggg caaccaaauu     3960

uuccuuuccc agaaguuugu gaaacugcug uaucacgcca agcgcaucuc uaauacaauu     4020

aacgagaauc aucggaaaua cguggaaaac cacaagaaag aguucgagga acucuuuuac     4080

uauauccugg aguucaauga aaacuacguc ggggcgaaga agaacggaaa acuucugaau     4140

agcgcuuuuc aguccuggca gaaccacucu aucgacgagc ucuguagcuc cuucauuggc     4200

ccuaccgggu cugaaagaaa gggccuguuu gagcucacga gccggggauc cgccgccgau     4260

uucgaguucc ugggcguuaa aaucccccgc uauagagacu acacaccgag cucucuucug     4320

aaggaugcua cccugauuca ucaauccgug acagggcucu augaaacccg gaucgaccug     4380

gccaaacuug gagagggcaa gcgcccugcg gcuaccaaga aagccgggca ggccaagaaa     4440

aagaaauaag gacuaguccc uguucccaga gcccacuuuu uuuucuuuuu uugaaauaaa     4500

auagccuguc uuucagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       4589


<210>  4814
<211>  3665
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4814
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccugguac      180

gcgagccuga uguccgcuca ucaccuccgg guuggaauug acgugggcac ccauucugug      240

gggcuggcca cacuucgcgu cgaugaccac ggaacgccga ucgagcugcu cagcgcccug      300

ucccauauuc acgauucugg cguggggaag gaagggaaaa aggaccacga uaccagaaag      360

aaacuuagcg gcaucgcucg gcgcgccaga cggcugcucc aucaccgcag aacacagcug      420

caacagcugg acgagguucu ccgggaucug ggcuucccca uuccuacccc cggggaauuu      480

cuugaccuga acgagcagac agauccuuau cgcgugugga gaguccgggc ucgccucgug      540

gaagagaagc ugcccgagga acuuagagga ccugccaucu ccauggcggu ucggcauauc      600

gcccgccaca gaggcuggcg gaauccguac ucuaaagugg agagccugcu cucccccgcu      660

gaagagagcc cuuucaugaa ggcccugaga gagcgcauuc uggcuaccac aggggaaguc      720

cucgacgaug gaaucacccc cggccaggcc auggcgcaag uggcccugac gcacaacauu      780

ucuaugcggg ggccugaggg aauccuuggc aaacugcauc aguccgacaa ugcuaacgaa      840

auuagaaaga ucugcgcccg ccagggcgug agccccgaug ucuguaagca gcuccugcgg      900

gcuguuuuua aagccgacuc uccgagaggg uccgccguga gccgcguggc uccugacccc      960

cuuccuggac aaggcucuuu ccggagagcg cccaagugcg aucccgaguu ucagcgcuuc     1020

cggauuaucu ccaucgucgc caaucugaga auuagcgaaa ccaaagggga gaaccgcccu     1080

cucacagcug acgagcggag acacgugguu accuuucuga cagaagauuc ccaggccgac     1140

cugaccuggg uggaugucgc cgagaagcuc ggagugcauc ggcgcgaccu gagaggcacg     1200

gcuguucaca cagaugacgg ggaacggucu gccgcgagac cgcccaucga ugcuaccgac     1260

cgcauuaugc ggcagacaaa aaucagcucc cuuaagaccu ggugggagga agccgauagc     1320

gagcaaagag gagccaugau ucgcuaucug uacgaggacc cuaccgacuc ugaaugugcu     1380

gagaucaucg ccgaacuccc cgaggaagau caggcuaaac uggacucccu ucaucugccu     1440

gccggcagag cggccuauag ccgggagucu cucacagcuc ugagcgauca caugcuggcc     1500

acgaccgacg aucuccacga ggcucgcaag agacuguucg gcguggacga uuccugggcg     1560

cccccggccg aagccauuaa ugcuccuguc gggaaccccu cuguggaccg gacacuuaag     1620

aucgucggac gcuaccugag cgccguggag uccauguggg gcaccccuga aguuauucau     1680

guggagcacg ucagagaugg guuuacaagc gagcggaugg cugacgaacg cgauaaagcc     1740

aauagacggc gcuauaacga caaucaggag gccaugaaga aaauccaaag agauuacgga     1800

aaggaaggcu acauuucucg gggagacauc gugagacucg acgcucugga gcugcagggc     1860

ugcgcguguc uuuauugcgg aaccacgauc ggcuaccaua caugucagcu cgaucacauu     1920

guuccccagg ccgggccugg auccaacaau cgccggggca accugguggc ugucugcgaa     1980

agauguaauc gcagcaaauc uaacaccccc uucgccgugu gggcccaaaa gugcgggauc     2040

ccgcacgugg gaguuaagga ggcuauuggc cgggucagag gguggcgcaa acagaccccu     2100

aacacaucca gcgaggaccu gacccggcuc aagaaagaag ugaucgccag acugcgccgg     2160

acacaggagg aucccgaaau ugacgagaga uccauggaau cuguggcgug gauggcuaau     2220

gagcuucauc accggaucgc cgccgcuuau cccgagacga ccgucauggu guaccgcggc     2280

agcauuacag ccgcggcuag aaaggccgcc ggaaucgauu cccggaucaa ccugauuggc     2340

gaaaaagggc gcaaggacag aaucgaucgg agacaucacg cuguugacgc cucugugguc     2400

gcucucaugg aggccagcgu ggcgaagacc cuggccgaac gcuccagccu gcggggagag     2460

cagagacuua caggcaaaga acaaaccugg aagcaguaua cggggucuac cguuggagcu     2520

cgcgagcauu uugagaugug gcggggccac augcuccacc ugacagaacu guucaaugag     2580

agacucgccg aagauaaagu guacgucacc cagaacauuc gccugcggcu uuccgacggg     2640

aaugcucaua cagugaaccc uagcaagcug guuucucaca gacucggcga uggacugacc     2700

gugcagcaaa ucgaccgcgc cuguacgccg gcccuguggu gcgcucuuac aagagagaaa     2760

gauuuugacg agaagaaugg ccuccccgcg cgggaagacc gcgccauuag aguccauggg     2820

cacgagauca aguccagcga uuauauccag guguucucua aacggaagaa aaccgacagc     2880

gaucgcgacg aaacaccuuu cggagcuauu gccgugagag gcggguuugu cgagaucgga     2940

cccuccauuc aucacgcccg gaucuacaga guugaaggca agaaaccugu guacgcuaug     3000

cugcgcgugu ucacccacga ucugcucagc cagcggcaug gggaccuguu uucugccguc     3060

auucccccuc aguccaucag caugagaugc gcggagccca aacuucgcaa agcuaucacc     3120

acaggcaacg ccacguaucu gggaugggug guugugggcg augagcucga aauuaauguc     3180

gacuccuuca ccaaguacgc caucgggcgg uuucuggagg auuucccgaa cacaaccaga     3240

uggcggauuu guggauauga cacaaauucu aagcugaccc uuaaaccuau cgugcucgcu     3300

gccgaaggcc uggagaaccc cagcuccgcg guuaaugaaa uuguggagcu gaaggggugg     3360

cgcgucgcua ucaacgugcu cacgaaaguu cacccuacag uggucagacg ggaugcccug     3420

ggacgcccca gauacucuag ccgguccaau cuuccgaccu cuuggaccau cgagaagcgc     3480

ccugccgcua caaagaaggc cggccaagcu aagaaaaaga aauaaggacu agucccuguu     3540

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

gaauu                                                                 3665


<210>  4815
<211>  4526
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4815
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaccaag      180

ccguacagca uuggacugga caucggcaca aacuccguug ggugggcggu gacgaccgau      240

aauuauaaag ugcccucuaa gaagaugaaa guccucggaa acacaagcaa gaaauacauu      300

aagaaaaauc ugcuuggcgu gcuccuguuc gacuccggga ucaccgcuga gggacggcgc      360

cugaagagaa cagcccggcg cagauauacc cggcgcagaa accggauucu uuaccugcag      420

gaaaucuuuu cuacagagau ggccacccuc gaugacgcuu ucuuucaacg ccuggaugac      480

agcuuccugg uuccugauga caagagagau uccaaauauc ccaucuuugg caaucucgug      540

gaagagaagg ccuaccauga cgaguucccu acgauuuauc aucugcggaa auaccuugcu      600

gauucuacca agaaagccga ccugcgccuc gucuaccugg cgcuugccca caugaucaag      660

uauagaggcc acuuucugau ugagggggag uucaacagca aaaauaacga uauccagaag      720

aauuuucagg acuuccucga cacauacaac gcuauuuuug aauccgaucu gagccuggaa      780

aauucuaagc agcucgaaga gaucgugaaa gacaagauuu ccaaacugga gaagaaagau      840

cggauccuua agcuguuccc cggagaaaag aacagcggca ucuuuucuga guuccucaaa      900

cugauuguug ggaaucaagc cgacuuccgc aagugcuuua accuugauga aaaggcuucc      960

cugcauuuca gcaaagaguc uuaugacgaa gaucucgaga cccugcuggg auacaucggc     1020

gacgauuauu ccgacguguu ucucaaagcc aagaagcugu acgaugcgau ucuucugagc     1080

ggguuccuca cagucaccga caaugagacg gaagccccuc uguccucugc uaugaucaaa     1140

agauauaacg agcacaagga agaccuugcc cugcucaaag aguacauucg gaacaucagc     1200

cugaagacau acaaugaagu guuuaaagau gacaccaaga acggauaugc uggcuacauc     1260

gauggcaaga caaaucagga ggacuucuau guguaccuga aaaagcuccu ggccgaguuu     1320

gaaggggccg auuauuuccu ugagaaaauu gacagagaag auuuucugcg caagcagcgg     1380

accuucgaca acggauccau cccguaccag auucaccucc aagagaugag agcuauccug     1440

gauaaacagg cgaaguuuua ucccuuccug gccaaaaaua aggaacgcau ugagaagauc     1500

cuuaccuuuc ggaucccuua cuacgucggc ccccucgcua gagggaacag cgacuucgcc     1560

uggucuauuc gcaaacggaa ugagaagauc acaccuugga acuucgaaga uguuauugac     1620

aaagagucca gcgccgaggc uuuuaucaau agaaugacgu cuuucgaucu guaucugccc     1680

gaagagaagg ugcucccgaa acauagccug cuuuacgaaa ccuuuaacgu guauaaugag     1740

cugacaaagg uccgcuucau ugccgaaucc augcgggacu accaguuucu cgacucuaag     1800

cagaaaaagg auaucgugag acuguauuuc aaagacaagc gcaaaguuac cgauaaggac     1860

auuaucgagu accugcacgc gaucuaugga uacgauggca uugaacuuaa ggggaucgag     1920

aaacaauuua acagcucccu cagcacauac caugaccugc ugaauauuau caacgauaag     1980

gaguuccucg acgauucuuc caaugaagcu auuaucgagg aaaucauuca cacccugacg     2040

aucuuugagg accgggaaau gauuaaacag agacuuagca aguucgagaa caucuuugau     2100

aaaucugugc ugaagaagcu cucccggcgc cacuauacag gauggggcaa acugagcgcc     2160

aagcugauua acggcaucag agacgagaaa uccgggaaua ccauccuuga uuaccucauu     2220

gacgacggaa ucucuaaccg gaauuucaug cagcugauuc augaugacgc ccugagcuuu     2280

aagaaaaaga uccaaaaagc ucagauuauc ggcgaugaag acaaggggaa caucaaggag     2340

gucgugaaau cccucccugg aucucccgcc auuaagaaag gcauccugca gagcauuaag     2400

aucguugaug aacuugugaa agucaugggc gggagaaagc cugaguccau uguggucgaa     2460

auggcucgcg agaaucagua uaccaaccaa ggaaagagca auucucagca gcggcugaaa     2520

agacucgaga agucccugaa agaacugggc agcaagaucc uuaaagagaa cauucccgcc     2580

aagcucucua agaucgacaa uaacgcgcug cagaaugauc gccuguaccu cuauuaccug     2640

caaaacggga aagacaugua uacaggagau gaccuugaua ucgacagacu guccaauuac     2700

gacauugauc acaucauucc ccaggccuuc cucaaggaca acagcaucga uaauaaagug     2760

cugguuucua gcgcuuccaa ccggggcaag agcgacgaug ugccuucucu ggaagucgug     2820

aaaaagcgca agaccuucug guaccagcuu cugaaaucca agcucauuag ccagagaaaa     2880

uuugacaacc ugacaaaggc cgagcgcggg ggacucuccc cggaggauaa agcuggcuuc     2940

auccaacggc agcugguuga aacgagacag aucaccaagc auguggcccg gcuucuggac     3000

gagaaguuua auaacaaaaa ggaugaaaau aaccgcgcgg ucagaacagu gaaaauuauc     3060

acccucaagu cuacacuggu gagccaguuc cggaaagacu uugagcugua uaagguucgc     3120

gaaauuaaug auuuccacca ugcccacgac gcuuaccuua acgccgucgu ggcuuccgcc     3180

cugcucaaaa aguaucccaa gcuggagccu gaguuugugu acggggauua ucccaaauac     3240

aauucuuuca gagaaagaaa gagcgccacc gagaaagucu auuuuuacuc caacaucaug     3300

aauauuuuca agaaaucuau cagccucgcu gacggccggg ugaucgaacg cccucugauu     3360

gagguuaacg aagagacggg agaguccgug uggaauaagg aaagcgaccu ugcgaccguc     3420

cggagagugc ugucuuaccc ccaaguuaac guggucaaga aaguggagga acagaaucac     3480

ggccucgauc gcgggaagcc gaaaggacug uuuaacgcca aucuguccag caagccuaaa     3540

cccaacucua acgagaaucu uguuggcgcu aaggaauauc uggacccuaa gaaauacggg     3600

ggauaugccg gcaucuccaa cagcuucaca gugcucguca aggggaccau ugagaaaggc     3660

gccaagaaaa agaucacaaa ugugcuggag uuccagggaa uuucuauccu cgaucggauu     3720

aacuacagaa aggacaaacu gaauuuucuu cuggaaaagg gcuauaaaga uaucgagcug     3780

aucauugaac uccccaagua cucccuguuc gagcuuagcg acgggucccg ccggaugcug     3840

gcuagcaucc ucucuaccaa caauaaaaga ggagagauuc auaagggcaa ccaaaucuuu     3900

cugagccaga aauucgugaa gcuccuguau cacgccaagc gcauuuccaa uacgaucaac     3960

gaaaaucauc ggaaauacgu cgagaaccac aagaaagaau uugaggaacu uuucuacuau     4020

auccuggagu uuaaugagaa cuacguuggg gcgaagaaaa auggaaagcu gcucaacucu     4080

gcuuuccaga gcuggcagaa ucacuccauu gaugaacugu guucuagcuu uaucggcccu     4140

acaggguccg agagaaaggg ccuuuucgaa cugaccucuc ggggaagcgc cgccgacuuu     4200

gaguuccucg gcgugaaaau uccccgcuau agagauuaca caccguccag ccugcucaag     4260

gacgcuaccc ugauccauca aucugugacc gggcuuuaug aaacacggau ugaucuggcc     4320

aaacugggag agggcaagcg cccugcggcu acgaaaaagg ccgggcaggc caagaaaaag     4380

aaauaaggac uagucccugu ucccagagcc cacuuuuuuu ucuuuuuuug aaauaaaaua     4440

gccugucuuu cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa agaauu                                          4526


<210>  4816
<211>  3608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4816
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccgcggcu      180

uucaagccga acagcauuaa uuacauccug ggacucgaca uuggcaucgc cuccguuggg      240

ugggccaugg uggagauuga ugaagaggaa aaccccaucc ggcugaucga ccuuggagug      300

cgcgucuuug agagagcuga agugccuaaa accggcgauu cucuggccau ggcucggcgc      360

cucgccagaa gcguucggcg ccugacaaga cggcgcgcgc auagacuucu gcggacgcgc      420

agacuccuga agcgggaggg ggugcugcag gccgcuaauu ucgacgagaa cggacucauu      480

aagucccugc ccaauacccc uuggcaacuu cgcgccgcug cccuggauag aaaacucaca      540

ccccuggaau ggucugcggu ccuucugcac cucaucaagc aucggggcua ucugagccag      600

cgcaaaaacg agggcgaaac cgccgacaag gagcuggggg cucuccugaa aggaguggcc      660

ggcaaugcuc acgcccuuca gacaggggau uuuagaaccc cugccgagcu ggcucucaac      720

aaguucgaaa aggaguccgg acauauucgg aaucagagau cugacuacag ccacacauuu      780

ucccgcaaag aucugcaagc ggaacuuauc cuccuguucg agaagcagaa agaauuuggc      840

aacccgcacg uuagcggggg acugaaggag ggcauugaga cccugcucau gacgcagcgg      900

cccgcccugu cuggcgacgc ugugcagaaa augcuugggc auugcaccuu cgaaccugcc      960

gagcccaagg ccgcuaaaaa uacauauacc gccgaaagau uuaucuggcu gacaaagcuc     1020

aacaaucugc gcauucuuga gcaaggaucc gaacggccuc ugaccgauac ggagagagcg     1080

acacucaugg acgagcccua ccgcaagagc aaacugaccu augcucaggc ccggaagcug     1140

cucggccugg aagauacagc cuucuuuaaa gggcuuagau acggaaagga caacgcugag     1200

gccucuaccc ugauggaaau gaaggcuuau cacgccaucu cccgcgcgcu cgagaaggaa     1260

ggccugaagg acaaaaagag cccgcugaau cuuucuccug agcuccagga ugagaucggg     1320

accgccuucu cccuguuuaa aacagacgaa gauauuacgg gacggcugaa ggacagaauc     1380

cagcccgaga uucucgaagc ucugcuuaaa cauaucagcu ucgauaaguu cguccaaauu     1440

ucccugaagg cccuccggcg caucgugccu cugauggagc agggcaaaag auacgacgag     1500

gcuugugccg aaaucuacgg cgaucacuau gggaagaaaa acaccgagga aaagauuuac     1560

cugccuccca uccccgcgga cgagauucgg aacccggugg uccuuagagc ccucucucag     1620

gcucgcaaag uuaucaaugg aguggugcgg agauauggca gccccgcccg cauucacauc     1680

gaaacagcua gagaggucgg gaaguccuuu aaggaucgga aagagaucga aaagcgccaa     1740

gaggaaaaca gaaaagaccg ggagaaggcc gccgcuaaau uccgcgaaua cuuuccuaau     1800

uucgugggag agcccaagag caaagauauu cugaagcuga gacucuauga gcagcagcau     1860

ggcaagugcc uguacucugg gaaagaaauc aaccuuggcc ggcugaauga gaagggauau     1920

guugaaauug accacgcgcu cccuuuuucc cgcaccuggg acgauagcuu caacaauaaa     1980

gugcuggucc ugggcucuga gaaccagaau aaggggaacc aaacacccua cgaauacuuu     2040

aauggaaaag acaacagcag agaguggcag gaguucaagg cccgggugga aaccucccgc     2100

uuuccgagau cuaagaaaca gcggauccuu cuccagaagu ucgaugagga cggcuuuaaa     2160

gaaagaaauc ugaacgauac gcgcuauguu aaucgguucc ugugucaauu uguggcugac     2220

agaaugcgcc ucacagggaa gggcaaaaag cgggucuucg ccagcaacgg acagauuacc     2280

aaccugcuua gaggguucug gggccugcgc aaggugcggg ccgagaauga uagacaucac     2340

gcucucgacg ccgucguggu ugcgugcucc accguggcua ugcagcagaa aaucacacgc     2400

uuuguccggu acaaggagau gaacgccuuc gauggaaaaa ccauugacaa ggaaacaggc     2460

gaggugcugc aucagaaaac gcacuuuccu cagcccuggg aguucuuugc ccaggaaguu     2520

augaucagag uguucgggaa gccugaugga aagcccgaau uugaggaggc ugacacccug     2580

gaaaaacuuc ggacacuccu ggccgagaag cugagcucuc gcccugaagc gguccacgag     2640

uaugugacac cccucuucgu guccagagcu ccgaaucgga aaaugagcgg ccaggggcau     2700

auggaaaccg uuaagucugc caaacgccug gaugagggag ucuccgugcu uagagugccu     2760

cugacccaac ucaagcugaa agaccuggag aagaugguca accgggaaag agagcccaaa     2820

cuuuacgaag cccugaaagc ucgccucgag gcccacaagg acgaucccgc uaaagccuuu     2880

gcggaaccuu ucuauaagua cgacaaagcc ggcaaucgga cgcaacaggu gaaggcuguu     2940

agaguggagc aaguccagaa gaccggggug uggguucgca accauaaugg caucgccgau     3000

aacgcuacaa uggugcgggu cgacguguuu gagaaaggag auaaguauua ccugguuccg     3060

auuuacagcu ggcaaguggc caaaggcauc cuccccgaca gagccgucgu gcaggggaag     3120

gaugaagagg acuggcagcu gauugaugac uccuucaauu uuaaauucuc ucuucacccu     3180

aacgaucugg uggaagucau caccaagaag gcucgcaugu ucggauauuu ugcgagcugu     3240

caccggggca cagggaauau uaacaucaga auccaugacc ucgaucacaa aauuggaaag     3300

aauggcaucc uggaggggau uggcguuaaa accgcccugu ccuuccagaa guaccagauc     3360

gacgagcuug gaaaagaaau ucgccccugc agacugaaga agcggccucc cgugcgcaaa     3420

agaccugcug ccacgaagaa agccggccag gcuaagaaaa agaaguaagg acuagucccu     3480

guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaagaauu                                                              3608


<210>  4817
<211>  4364
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4817
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaagaaa      180

ccguacacca uuggacugga caucggcaca aacagcguug ggugggcggu gcucacggau      240

caguaugacc uggugaagcg gaagaugaaa auugcuggag auuccgagaa gaaacaaauc      300

aagaaaaauu ucuggggcgu ccgccuuuuu gacgaagggc agaccgccgc cgauagacgg      360

auggcucgca cagccagacg gcgcauugag agacggcgca acagaaucuc uuaccugcag      420

ggaaucuucg cugaagagau gagcaagacc gacgccaauu ucuucugccg gcucuccgau      480

ucuuuuuaug uggacaacga aaagcgcaau agcagacauc ccuucuuugc gacaauugag      540

gaggaaguug aguaccacaa aaacuauccu accaucuacc aucugcggga ggaacuuguc      600

aauuccucug agaaggccga ucugcgccuc gucuaucugg cucuggccca cauuaucaaa      660

uacagaggca acuuccucau ugaaggcgcu cuggacacac agaauaccag cguggauggg      720

aucuacaagc aauuuauuca gacguauaac cagguuuucg ccuccggaau cgaggacggc      780

agccuuaaaa agcuggaaga uaauaaagac guggcgaaga uccucgucga gaaggugacc      840

cggaaagaaa agcuggagcg cauucuuaaa cuguaccccg gggagaaguc ugccggaaug      900

uuugcucagu ucaucucccu cauugugggc agcaaaggga acuuucaaaa gccuuucgac      960

cugaucgaaa agucugauau ugagugugcc aaagacuccu augaagagga ucuggaaagc     1020

cuccuggcuc uuaucggaga cgaguacgcc gagcuguucg ucgccgcuaa gaaugcguau     1080

ucugccguug ugcucuccag caucauuaca guggcugaaa ccgagacaaa cgccaaacug     1140

uccgccucua ugaucgaaag auuugauacc caugaggaag accuuggcga gcugaaggcu     1200

uucauuaaac uccaucugcc caagcacuac gaggaaaucu uuagcaauac ggagaagcac     1260

ggcuaugccg gguacauuga uggaaaaaca aagcaggcgg acuucuacaa auauaugaag     1320

augacccugg aaaacaucga gggcgcugau uacuuuaucg ccaaaauuga gaaggaaaac     1380

uuccuccgga agcagagaac auuugacaau ggggccaucc cucaccagcu gcaucuugag     1440

gaacuggagg cuauucucca ccaacaggcc aaauauuacc cguuccugaa ggaaaacuau     1500

gauaaaauca agucccuugu caccuuucgc auucccuacu ucgugggacc ucuggcuaau     1560

ggccagagcg aguuugccug gcucacccgg aaagcggacg gggagaucag acccuggaac     1620

auugaagaga agguugacuu cggaaaaucu gccguggauu uuaucgaaaa gaugacaaau     1680

aaggacacgu aucugccuaa agagaacguc cugcccaagc acucccucug cuaccaaaaa     1740

uaccuggugu auaaugaacu uaccaagguu cgcuacauca acgaucaggg caaaacaagc     1800

uauuucucug gccaggagaa ggagcagauu uucaaugacc uguuuaagca aaaacggaag     1860

gugaaaaaga aagaucucga acuguuccug agaaacauga gccaugucga guccccgacc     1920

aucgaagggc uugaggacuc uuuuaauagc uccuacagca cauaucacga ucuccugaag     1980

gugggaauua agcaggaaau ccuggacaac ccugucaaua ccgagaugcu cgagaacauu     2040

gugaaaaucc ugacgguuuu cgaagauaag cgcaugauca aagagcagcu ucagcaauuu     2100

ucugacgugc uggauggcgu cgugcucaag aaacuggaac ggagacauua cacagggugg     2160

ggacgccugu ccgcuaagcu ccucaugggc auucgggaca agcagagcca ccugaccauc     2220

cuggauuauc ucaugaauga cgacgggcug aacagaaacc uuaugcagcu gauuaaugau     2280

ucuaaccucu ccuucaaaag caucauugag aaggagcagg uuaccacagc cgacaaagau     2340

auccaaucca ucguggcuga ccuggccggc ucucccgcga uuaagaaagg aauccugcag     2400

agccuuaaga uugucgauga acucgugucc gugaugggcu acccucccca gaccaucguu     2460

gucgagaugg cccgggaaaa ucagacaacg gggaaaggaa agaacaauuc ucgccccaga     2520

uacaagagcc uggagaaagc uauuaaggaa uuuggcuccc aaauccugaa agagcauccu     2580

accgacaacc aggagcuccg gaauaacaga cuguaucuuu acuaucugca gaaugggaag     2640

gauauguaca caggacaaga ccucgauauc cacaaccuga gcaauuauga cauugaucac     2700

aucgugccgc agucuuucau uaccgacaac uccaucgaua aucuggugcu uacaagcucu     2760

gccggcaacc gcgaaaaagg ggacgacgug cccccucucg agauugugcg gaagagaaag     2820

guguuuuggg aaaaacugua ccagggcaau cugaugucca agcgcaaauu cgauuaucuc     2880

accaaggcug agagaggagg ccugacggaa gccgacaaag cccgguuuau ccaucgccag     2940

cuuguugaga ccagacaaau uacaaagaac gucgcuaaca uccugcacca gcgguucaau     3000

uacgagaagg augaccaugg gaacaccaug aaacaggugc gcaucguuac acucaagagc     3060

gcgcuggugu cucaguuuag aaaacaauuc cagcuguaca agguccggga ugugaaugac     3120

uaucaccacg cccaugaugc uuaccuuaac ggaguugugg ccaauacccu gcucaaaguc     3180

uauccccagc uggaaccuga guucguguac ggcgacuauc accaguuuga uugguucaag     3240

gccaacaagg cuacggccaa aaagcaauuu uacacaaaua uuaugcuuuu cuuugcgcag     3300

aaagaccgca ucauugauga aaacggggag auccuguggg acaagaaaua ucuugacacc     3360

gugaagaagg ucaugagcua cagacagaug aauauuguua aaaagacaga aauccaaaaa     3420

ggagaguucu ccaaggcuac caucaaaccc aagggcaaca gcucuaaacu gauuccgcgg     3480

aagaccaauu gggauccuau gaaguacggg ggccucgacu cccccaacau ggccuaugcc     3540

guggugaucg aguacgcuaa aggaaagaau aaacuggucu uugaaaagaa aauuauccgc     3600

gugacaauua uggagagaaa ggccuucgaa aaggaugaga aagcguuucu ggaggaacag     3660

ggcuaucggc agccuaaggu ucuugcuaaa cugcccaagu acacgcucua ugagugugaa     3720

gaggggagac gccggaugcu ggccagcgcc aacgaagcuc agaaaggaaa ucaacaggug     3780

cucccuaacc aucuggucac ccuucugcac caugccgcua acugcgaggu guccgacggc     3840

aagucucucg auuacaucga gagcaauaga gaaauguucg ccgagcugcu ggcgcacguu     3900

uccgaauuug ccaagcgcua uacacuugcu gaggccaacc ugaauaaaau caaccagcuc     3960

uuugaacaga auaaggaggg ggacauuaaa gcuaucgccc aaucuuucgu ggaucugaug     4020

gccuuuaacg cuaugggagc gcccgccagc uucaaguuuu ucgagaccac aauugaacgg     4080

aaaagauaca auaaccucaa ggagcugcuu aauuccacca ucauuuacca gucuaucacg     4140

ggccuguaug aaagccgcaa gcggcuggac gauaaaagac cggcugccac aaagaaagcc     4200

gggcaggcua agaaaaagaa auaaggacua gucccuguuc ccagagccca cuuuuuuuuc     4260

uuuuuuugaa auaaaauagc cugucuuuca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4364


<210>  4818
<211>  4397
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4818
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaagaaa      180

ccguacagca uuggacugga caucggcacc aacuccguug ggugggcggu ggugacagau      240

gacuauaagg uccccgcuaa gaaaaugaag gugcucggaa auacggauaa aucucauauu      300

gagaagaacc ugcuuggcgc ccuccuguuc gacagcggga auaccgccga agaucggcgc      360

cugaaaagaa cagcucggcg cagauacacc cggcgcagaa accggauccu uuaucugcag      420

gagauuuucu ccgaagagau gggaaagguu gacgauucuu ucuuucaccg ccucgaagac      480

agcuuccugg ugacagagga uaagagaggc gagcggcauc cuaucuuugg caaucuggaa      540

gaggaaguca aauaccacga gaacuucccc accaucuauc aucuccgcca auaccuggcc      600

gacaauccug agaaggugga ucuuagacug guuuaucucg cucuggccca cauuaucaaa      660

uuucgggggc acuuccuuau ugaaggaaag uuugacacac gcaacaauga ugugcagaga      720

cuguuccagg aguuucucgc ggucuacgac aacaccuucg aaaauuccuc ucugcaggag      780

caaaacgucc agguggaaga gauccugacg gacaaaauua gcaaguccgc caaaaaggau      840

cgggugcuca agcuguuucc caaugagaaa agcaacggca gauucgcuga auuucuuaag      900

cugaucguug ggaaucaggc cgacuuuaaa aagcauuucg agcucgaaga gaaagcuccu      960

cugcaguuuu cuaaggauac cuacgaagag gagcuugaag ugcugcucgc ccaaauugga     1020

gacaacuaug cggagcuguu ccuguccgcc aagaaacucu acgauagcau ccugcuuucu     1080

ggcauccuga cagugaccga cgucgggaca aaggcuccgc ucuccgccag caugauucag     1140

cgcuauaacg aacaccagau ggaucuggcu cagcuuaaac aauuuauccg gcagaagcug     1200

ucugacaaau acaaugaggu guucuccgau guuagcaagg acggauaugc cggcuacauu     1260

gauggcaaga ccaaccagga agccuuuuau aaauaccuca aggggcugcu gaauaaaauc     1320

gagggauccg gcuacuuccu cgacaagauu gagagagaag acuuucugcg caaacaacgg     1380

acguucgaua acgggucuau cccccaucag auccaccuuc aggagaugag agcuauuauc     1440

cgccggcagg cggaauuuua uccuuuccug gccgacaauc aagauagaau ugagaagcuc     1500

cugacauuuc gcauccccua cuauguggga ccucuggcuc ggggcaagag cgacuucgcc     1560

uggcuuucca gaaaaagcgc cgauaagauu acccccugga acuucgacga gaucgucgau     1620

aaagaaucuu ccgcugaggc cuuuaucaau cgcaugacaa acuacgaccu cuaucugccg     1680

aaucagaagg ugcugccuaa acacagccuc cuguacgaaa aguucaccgu uuauaacgag     1740

cuuaccaaag ugaaguacaa gacagaacag gggaaaacgg cguuuuucga ugcuaauaug     1800

aagcaggaga uuuuugacgg agucuucaaa guguaccgga aggucaccaa agauaagcug     1860

auggacuuuc ucgagaagga guucgaugaa uuuagaaucg uggaccugac aggccuggac     1920

aaagaaaaca agguuuucaa ugccucuuau ggcaccuacc augaucuuug caaaauucuc     1980

gacaaggauu uucuggacaa cagcaaaaau gagaagaucc uggaagauau ugugcucaca     2040

cugacccuuu ucgaggaccg ggagaugauc cgcaagagac uggaaaacua uuccgaucuc     2100

cugacgaaag agcaagucaa gaaacuggaa cggagacacu acacagggug gggacgccuu     2160

ucugccgagc ucauucaugg cauccggaac aaggaaagca gaaaaaccau ccuggacuau     2220

cugauugaug acggaaauuc caaccgcaau uuuaugcagc ucaucaacga ugacgcucug     2280

agcuucaagg aggagauugc caaggcucag gugaucgggg aaaccgauaa ucuuaaccag     2340

guugugucug acauugccgg cucccccgcg aucaaaaagg ggauccugca aagccucaaa     2400

auugucgacg agcuggugaa gaucaugggc caccagccug aaaauauugu gguugagaug     2460

gccagagaga accaguucac aaaucaggga cggcgcaacu cucaacagag acugaaaggc     2520

cuuaccgauu ccaucaagga auuugggagc cagauucuca aagagcaucc cgucgaaaau     2580

ucccaacugc agaacgaccg gcuguuccuc uacuaucugc agaauggacg cgauauguac     2640

acaggcgagg aacuugacau cgauuaccug ucucaguaug acaucgauca cauuaucccc     2700

caggcuuuua uuaaggacaa cagcaucgau aauagagugc ucacguccuc uaaggagaac     2760

cgggggaaaa gcgacgaugu gccuuccaag gacgucgugc gcaaaaugaa aagcuacugg     2820

ucuaaacugc uguccgccaa acuuauuacc caaagaaagu ucgacaaccu gacaaaagcu     2880

gagcggggag gccucaccga ugacgauaag gccggguuua ucaaacgcca gcugguugaa     2940

acaagacaga ucaccaagca cguggcgcgg auucucgacg agagauucaa uacggaaacc     3000

gaugagaaca auaaaaagau ccgccaaguc aagauuguga cacugaaaag caaccuuguu     3060

ucuaauuuuc ggaaggaguu cgaacuguau aaagugagag agaucaacga cuaccaucac     3120

gcccaugaug cuuaucucaa ugccgucauu ggcaaggcuc ugcugggagu guacccgcag     3180

cuugaacccg aguuuguuua uggcgacuac ccucacuucc acgggcauaa agaaaacaag     3240

gccaccgcca agaaauuuuu cuacuccaau aucaugaacu ucuuuaagaa agaugacgug     3300

cgcacagaua agaauggaga gauuaucugg aaaaaggacg aacacaucag caacauuaag     3360

aaaguccugu cuuaucccca ggugaauauc gugaagaaag ucgaggagca gaccggcggg     3420

uucagcaagg aauccauucu cccuaaagga aacagcgaua agcugauccc ccggaaaacg     3480

aagaaguuuu acugggacac aaagaaguau ggcggguucg acucuccgau uguugcuuac     3540

uccauccucg ugaucgcgga uauugagaaa ggcaagagca aaaagcugaa gaccgugaaa     3600

gcccuugucg gagugacaau cauggaaaag augaccuuug agagagaccc uguugcuuuc     3660

cuggagcgca aaggcuaucg gaaugugcaa gaagagaaca uuaucaagcu ccccaaauac     3720

ucccuguuua agcuggaaaa cgggagaaag cgccuucugg ccucugcccg ggagcuccag     3780

aaaggaaaug aaauuguccu gccuaaccau cucggcaccc ugcuuuauca cgcuaagaau     3840

auccauaaag uggaugagcc caagcaccug gacuacguug auaaacacaa ggacgaguuc     3900

aaggaacugc ucgauguggu cagcaacuuu uccaaaaagu acacacuggc cgaggggaau     3960

cuugaaaaga ucaaggagcu guaugcgcag aacaauggag aagaccucaa agagcuggcu     4020

ucuagcuuca uuaaccuccu gacguuuacc gccaucggcg ccccugcuac auucaaguuu     4080

uucgauaaaa auauugacag aaagcgguac accuccacaa ccgagauccu uaacgccacg     4140

cugauucauc aaucuaucac agggcuguau gaaacccgca uugaucucaa uaagcugggc     4200

ggagacaaaa gacccgcggc uaccaagaaa gccggccagg ccaagaaaaa gaaguaagga     4260

cuagucccug uucccagagc ccacuuuuuu uucuuuuuuu gaaauaaaau agccugucuu     4320

ucagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aagaauu                                                    4397


<210>  4819
<211>  3530
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4819
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugcccagaca      180

acaaaccuga gcuacauucu cggacuggac cuuggcaucg cguccguugg gugggcugug      240

guggagauua augaaaacga ggauccgauc ggacugauug acgucggcgu gcggaucuuc      300

gaacgcgccg agguucccaa gacgggggaa ucucucgccc ugagcagacg gcuugcucgc      360

uccaccagac ggcugauccg cagacgggcc caucgccucc ugcuggcuaa aagauuucuc      420

aagcgggagg ggauucuguc uacaaucgau cuugagaagg gccugccuaa ucaagccugg      480

gaacuccgcg uggcgggccu ggagagacgg cuuagcgcca uugaaugggg agcuguccug      540

cuccaccuga ucaaacaucg cggauaucug uccaagcgga aaaacgaguc ucagaccaau      600

aacaaggagc ucggcgcccu gcuuagcggg guggcucaga aucaccagcu gcuccaaucc      660

gacgauuaca gaacacccgc cgaacuggcg cuuaaaaagu ucgccaagga ggaaggacau      720

auuagaaacc agcgcggcgc uuauacccac acauuuaauc ggcuggaccu ccuggccgag      780

cugaaccucc uguucgcuca gcagcaccaa uuugggaauc cucauugcaa agaacacauc      840

cagcaguaca ugaccgagcu ucugaugugg cagaagcccg cccucagcgg agaggccauu      900

cugaaaaugc uuggcaagug uacgcaugaa aagaacgagu ucaaagcugc gaaacacacc      960

uauucugccg aaagauuugu uuggcugaca aagcucaaua accugcgcau ccuggaggau     1020

ggcgcugaac gggcccucaa ugaggaggaa agacaacugc uuaucaacca cccuuacgag     1080

aaguccaaac ugaccuaugc ccaggugcgc aagcuccugg ggcugagcga acaggcuauu     1140

uucaaacauc uucgguacuc uaaggagaac gccgaauccg cgacauuuau ggagcucaag     1200

gcuuggcacg ccaucagaaa agcccuggag aaucaaggac ugaaggacac cuggcaggau     1260

cucgcuaaaa agccggaccu gcuugaugaa auuggcacgg ccuucagccu guacaaaaca     1320

gacgaggaua uccagcagua ucucaccaac aaggucccca auucugugau uaacgcucug     1380

cugguguccc uuaauuuuga caaauucauc gaacucagcc ugaagucccu gcgcaagauc     1440

cucccucuga uggagcaagg gaaacgguac gaccaggccu gcagagagau uuauggacau     1500

cacuacggcg aagcgaacca gaagacaucu cagcuucugc ccgccauccc ugcucaagag     1560

auucgcaauc ccgucguucu ccggacccug agccaggcca gaaaagugau caacgcuauu     1620

auccggcagu augggucccc ggcccgcgug cauaucgaaa ccggaagaga gcugggcaag     1680

agcuuuaaag aacggagaga gauucagaag caacaggagg auaaucgcac aaagcgggaa     1740

ucugcggucc agaaauucaa ggagcuuuuc uccgacuuua gcucugaacc uaaaagcaag     1800

gauauccuca aauucagacu guacgagcag caacacggca agugucugua uuccgggaaa     1860

gaaauuaaca uccaccgccu caaugagaag ggauacgugg agauugacca ugcccugccc     1920

uuuucuagaa cgugggauga cagcuucaac aauaagguuc uugugcuggc uuccgaaaac     1980

cagaauaaag gcaaccagac cccuuacgag uggcuccaag ggaagaucaa cagcgaacgg     2040

uggaaaaauu uugucgcccu ggugcuggga ucucagugcu ccgcugccaa gaaacagcgc     2100

cuucucacac agguuauuga ugacaacaag uucaucgaua gaaaucugaa cgacacccgg     2160

uauaucgccc gcuuucugag caauuacauu caagagaacc uccugcuugu gggcaagaau     2220

aaaaagaacg ucuucacacc caaugggcag aucaccgcuc ugcucagauc ucgguggggc     2280

cugauuaaag cgcgcgaaaa caauaacaga caccaugccc uggaugcuau cguggucgcc     2340

ugugccacgc ccuccaugca gcagaagauu acacgguuua uccgcuucaa agaggugcac     2400

ccuuauaaga ucgagaauag auacgaaaug guugaccaag agagcggaga aauuaucucc     2460

ccgcacuuuc ccgagccuug ggcuuauuuc cggcaggagg ugaacauuag agucuuugac     2520

aaucaucccg auaccgugcu uaaggaaaug cugccugacc gcccccaggc caaccaccag     2580

uucguucaac cucucuucgu gucucgggcg ccgaccagaa aaaugagcgg ccaggggcau     2640

auggagacaa ucaaguccgc uaaacgccug gccgaaggaa uuucuguccu ccggaucccc     2700

cugacccagc uuaagccuaa ccugcucgag aauaugguga acaaagaaag agagcccgcc     2760

cuguacgcug gccugaaggc ccgccuugcg gaguuuaauc aagauccugc uaaggccuuc     2820

gccacacccu uuuauaaaca agggggacaa caggugaagg cuauccgggu ugaacagguc     2880

cagaaaagcg gcgugcuggu gagagagaac aauggggucg ccgacaacgc uuccauugug     2940

cgcacggaug uuuucaucaa gaauaacaaa uuuuuccucg ugccgauuua caccuggcag     3000

gucgccaagg gcauccugcc uaauaaagcg auuguggccc acaagaacga agacgagugg     3060

gaagagaugg augagggagc uaaguuuaaa uucagccucu uucccaauga ccugguugaa     3120

cuuaagacca aaaaggagua cuucuuuggc uauuacaucg ggcuggaucg ggccacagga     3180

aacaucucuc ucaaagaaca ugacggcgag auuuccaagg ggaaggaugg aguguauaga     3240

gucggcguga aacuggcucu gagcuucgaa aaguaccagg uugacgagcu ugggaaaaau     3300

cggcagaucu gccgccccca gcagagacag ccugugcgga agcgcccggc cgccaccaaa     3360

aaggcuggcc aagcgaagaa aaagaaauaa ggacuagucc cuguucccag agcccacuuu     3420

uuuuucuuuu uuugaaauaa aauagccugu cuuucagauc uaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                3530


<210>  4820
<211>  3608
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4820
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccgcggcu      180

uucaagccga accccauuaa uuacauccug ggacucgaca uuggcaucgc cagcguuggg      240

ugggccaugg uggagauuga ugaagacgag aacccuaucu gccugaucga ucuuggagug      300

cgggucuuug aacgcgcuga ggugcccaaa accggcgacu cccuggccau ggcuagacgg      360

cucgcccgcu cuguuagacg gcugacacgc agacgggcgc aucgccuucu gagagcccgg      420

cgccuccuga agagagaagg ggugcugcag gcugccgauu ucgacgagaa uggacucauu      480

aagagccugc cuaacacgcc cuggcaacuu cgggcugccg cgcuggaucg caaacucacc      540

ccucuggagu gguccgccgu ccuucugcac cucaucaagc auagaggcua ucugucucag      600

cggaaaaaug aaggcgagac agcugacaag gaacuggggg cccuccugaa aggaguggcu      660

gauaacgccc acgcccuuca gaccggcgac uuuagaacac cggcugagcu ggcgcucaau      720

aaguucgaga aggaaagcgg gcauauucgc aaccagcggg gagauuacuc ccacaccuuu      780

ucuagaaaag accugcaagc cgagcuuauc cuccuguucg aaaagcagaa agaguuuggc      840

aauccccacg uuagcggggg acugaaggaa ggcauugaga cacugcucau gacccagcgc      900

ccugcucugu ccggcgaugc cgugcagaaa augcuugggc auuguacguu cgagcccgcc      960

gaaccuaagg cugccaaaaa caccuauaca gcggagcggu uuaucuggcu gaccaagcuc     1020

aauaaccugc gcauucuuga acaaggaagc gagagacccc ugacagacac cgaacgggcu     1080

acgcucaugg acgagccgua cagaaagucu aaacugacau augcccaggc ccgcaagcug     1140

cucggccugg aggauaccgc uuucuuuaaa gggcuucggu acggaaagga caaugccgag     1200

gcuuccacac ugauggaaau gaaagccuau cacgcgauca gcagagcccu cgaaaaggag     1260

ggccugaagg auaaaaaguc uccucugaac cuuucccccg aacuccagga cgagaucggg     1320

accgcuuuca gccuguuuaa aaccgaugag gacauuacag gacgccugaa ggaucggauc     1380

cagccugaaa uucucgaggc ccugcuuaaa cauaucucuu ucgacaaguu cguccaaauu     1440

ucccugaagg cucucagacg gaucgugccc cugauggaac agggcaaacg cuacgaugag     1500

gccugcgcgg agaucuacgg cgaccacuau gggaagaaaa auacggaaga gaagauuuac     1560

cugcccccua ucccggccga ugaaauuaga aaccccgugg uccuucgggc ucucagccag     1620

gccagaaaag uuaucaacgg aguggugcgc cgguauggcu ccccugcucg cauucacauc     1680

gagaccgcca gagaagucgg gaagucuuuu aaggacagaa aagagaucga gaagcggcaa     1740

gaagagaauc gcaaagacag agaaaaggcc gcugcgaaau uccgggagua cuuucccaac     1800

uucgugggag aaccuaagag caaagauauu cugaagcugc gccucuauga gcagcagcau     1860

ggcaaguguc uguacuccgg gaaagagauc aaucuuggca gacugaacga aaagggauau     1920

guugagauug accacgcccu ccccuuuagc cggacauggg augacucuuu caauaacaaa     1980

gugcuggucc ugggcuccga aaaucagaac aaggggaauc aaaccccgua cgaguacuuu     2040

aacggaaaag auaauagccg cgaauggcag gaguucaagg cuagagugga gacaucucgg     2100

uuuccucgca gcaagaaaca gagaauccuu cuccagaagu ucgacgaaga uggcuuuaaa     2160

gagcggaacc ugaaugacac cagauauguu aaccgcuucc ugugccaauu uguggccgau     2220

cggaugagac ucacggggaa gggcaaaaag cgcgucuucg ccuccaaugg acagauuaca     2280

aaccugcuuc ggggguucug gggccugaga aaggugcgcg cugaaaacga ccggcaucac     2340

gcccucgaug cggucguggu ugcuuguucu accguggcca ugcagcagaa aaucaccaga     2400

uuuguccgcu acaaggagau gaaugccuuc gacggaaaaa caauugauaa ggagaccggc     2460

gaagugcugc aucagaaaac acacuuuccc cagccuuggg aguucuuugc ucaggaaguu     2520

augauccggg uguucgggaa gcccgacgga aagccugagu uugaagaggc cgacacgccc     2580

gagaaacuga gaacccuucu cgcggaaaag cugagcuccc ggccggaggc uguccacgaa     2640

uaugugacac cucuguucgu gagccgcgcc cccaacagaa aaaugucugg ccaggggcau     2700

auggagaccg uuaaguccgc caaacggcuc gaugaaggag ucagcgugcu gcgcgugccc     2760

cuuacacaac ugaagcucaa agaccuggag aagaugguca auagagagcg ggaaccuaag     2820

cuguacgagg cucuuaaagc cagacuggaa gcucacaagg augacccggc caaagcguuu     2880

gccgagcccu ucuauaagua cgauaaagcu ggcaaccgca cccaacaggu gaaggccguu     2940

cggguggaac aaguccagaa gacgggggug uggguuagaa aucauaacgg caucgcugac     3000

aaugccacca uggugcgcgu cgauguguuu gagaaaggag acaaguauua ccucguuccu     3060

auuuacucuu ggcaaguggc caaaggcauc cugcccgauc gggcugucgu gcaggggaag     3120

gacgaggaag auuggcagcu cauugacgau uccuucaacu uuaaauucag ccugcacccu     3180

aaugaccuug uggaggucau cacaaagaag gcgagaaugu ucggauauuu ugccuccugc     3240

caccgcggca ccgggaacau uaauauccgg auccaugacc uggaucacaa aauuggaaag     3300

aacggcaucc ucgaagggau uggcguuaaa acagcucugu cuuuccagaa guaccagauc     3360

gacgagcugg gaaaagagau uagacccugu cgccuuaaga agagaccucc cgugcggaaa     3420

cgcccggccg ccaccaagaa agcuggccag gccaagaaaa agaaguaagg acuagucccu     3480

guucccagag cccacuuuuu uuucuuuuuu ugaaauaaaa uagccugucu uucagaucua     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaagaauu                                                              3608


<210>  4821
<211>  3614
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4821
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccaaguac      180

cauguuggaa uugacguggg caccuucagc guggggcugg cggcuaucga ggucgaugac      240

gccggaaugc cgauuaaaac acucucccug gugucucaca uccaugauag cggccuugac      300

cccgaugaaa uuaaguccgc cguuacgcgg cuggcuucua gcgggaucgc ccgcagaacc      360

cggcgccucu auagacggaa gcgcagacgg cugcagcaac uugacaaauu uauccagcgc      420

cagggauggc cugugauuga gcuggaagau uacuccgacc cccucuaucc uuggaagguc      480

agagcugagc uggccgcguc uuacaucgcc gaugaaaaag agcggggcga gaagcugagc      540

guggcucucc gccacauugc cagacaucgg ggcuggcgca accccuaugc uaaaguuucc      600

agccuguacc uuccugacgg gccgucugau gccuucaagg cgaucagaga agagauuaag      660

cgggccuccg gacagcccgu gccugaaaca gcuaccgucg gccaaauggu gacacugugc      720

gagcucggga cccugaaacu uagaggagag ggcggggugc ugagcgcccg ccuccagcag      780

ucugacuaug cucgggaaau ccaggagauu uguagaaugc aagaaaucgg acaggagcug      840

uaccgcaaga ucauugaugu cguuuuugcc gccgaauccc ccaaaggcag cgcuucuucc      900

cgggugggca aggacccucu gcagcccggg aaaaauagag cgcucaaggc cagcgacgcu      960

uuccagcgcu accggaucgc cgcccugauu ggaaaccuua gagugcgcgu cgauggcgag     1020

aaacggaucc uguccgugga ggaaaagaau cucguuuuug accaccuggu gaaccuuaca     1080

ccgaagaaag agccugaaug ggucaccauu gcugagaucc uggggaucga uagaggacaa     1140

cucauuggca cggccaccau gacagacgau ggggaacgcg cgggagcucg gcccccuacc     1200

cacgacacaa auagaucuau cgugaacagc cggauugcuc cccugguuga uugguggaag     1260

accgccuccg cccuggagca gcaugccaug gugaaagcuc ucagcaaugc cgaggucgac     1320

gauuucgacu cucccgaagg cgcgaaggug caggccuuuu ucgcugaucu ggacgacgau     1380

guccacgcca aacuugacuc ccugcaucuc ccugugggcc gcgcugccua uagcgaggau     1440

acgcugguua gacuuacacg gagaaugcug ucugacgggg uggaucucua caccgcgcgc     1500

cugcaagaau uuggaaucga gccgagcugg acacccccua caccccggau uggcgaaccu     1560

gucgggaacc ccgccgugga cagaguucug aagaccgugu cccgcuggcu cgagucugcu     1620

accaagacgu ggggagcccc ggagagaguc aucaucgaac acgugcggga gggcuucgug     1680

accgaaaaac gcgcuagaga gauggauggg gacaugcggc gcagagccgc ccggaaugcu     1740

aagcuguuuc aggagaugca ggaaaaacuu aacguucagg gcaagccuag ccgcgcggau     1800

cuguggagau aucaauccgu ccagcggcag aauugccagu gugccuacug cggaagcccc     1860

auuacauucu cuaacuccga gauggaccac aucgugccuc gcgcuggcca agggagcacc     1920

aauacaagag aaaaccucgu ggccgucugu caucggugca aucagucuaa aggaaacacc     1980

cccuuugcca uuugggcuaa gaacacgucc aucgagggcg ugagcguuaa ggaagccgug     2040

gagagaacac gccacugggu caccgauacc gggaugcggu ccacagacuu caaaaaguuc     2100

accaaagcgg ugguugagag auuucagcgc gcuacaaugg augaagagau ugacgcccgg     2160

ucuauggaaa gcguggccug gauggcuaau gagcugagau cccgcgucgc ccagcauuuc     2220

gcgucucacg gaacgaccgu gcggguuuau agaggcagcc ugacagcuga agcccgccgg     2280

gccuccggga ucagcggcaa gcugaaauuu uucgacggag ugggcaaguc uagacucgau     2340

cggcgccauc acgcuauuga cgccgcuguc aucgccuuua ccuccgauua cguggcggag     2400

acacuggccg ugagaagcaa ccugaagcaa ucucaggcuc accggcagga ggccccucag     2460

uggcgcgaau uuaccgggaa agacgcugag cauagagccg ccuggcgggu cuggugucaa     2520

aagauggaaa aacucuccgc ucugcuuacg gaggaucuga gagacgaucg cguuguggug     2580

augagcaaug uccggcucag acugggaaac ggcucugcgc acaaggaaac caucgggaaa     2640

cugagcaagg ugaaacuuuc cagccagcuc ucuguuuccg acauugauaa ggccagcucc     2700

gaggcucugu ggugcgcccu gacacgcgag cccggauuug acccgaagga aggccucccu     2760

gccaaucccg agcggcauau cagagugaac gggacccacg ucuaugcugg cgauaauauu     2820

ggacuguucc ccgugucugc cggcagcauc gcgcuucgcg ggggauacgc ugaacugggc     2880

uccucuuuuc accaugcccg gguuuacaaa auuacaagcg ggaagaaacc ugccuucgcu     2940

augcucagag uguauaccau cgaccugcug ccguaccgca accaggaucu uuucuccguc     3000

gagcucaagc cccaaacgau gucuaugaga caggccgaga aaaagcugcg ggacgcgcug     3060

gcuacaggaa augccgaaua ucucggcugg cuggugguug acgaugagcu uguggucgac     3120

accagcaaga ucgccacaga ucaggugaaa gcuguggaag ccgagcuggg gaccauucgc     3180

agauggcggg ucgacggcuu cuuuuccccu agcaagcucc gccugagacc ccugcagaug     3240

ucuaaagaag gaaucaagaa agaguccgcu ccugagcuua gcaagauuau cgaucggccc     3300

ggcuggcucc cugccguuaa caagcuguuc ucugacggga augugaccgu ggucagacgc     3360

gauucccugg gacgggugag acucgaaagc acagcgcacc uucccguuac guggaaagug     3420

caaaagcgcc cggccgcuac caaaaaggcc ggccaggcua aaaagaagaa auaaggacua     3480

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     3540

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaag aauu                                                       3614


<210>  4822
<211>  3314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4822
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccgcgcgg      180

auucuggcuu ucgacaucgg aauuagcucc aucggcuggg ccuuuucuga gaacgaugaa      240

cucaaggacu gcgggguucg cauuuucacc aaaguggaga auccgaagac aggagaaagc      300

cuggcccuuc ccagacggcu ggcucgcucc gccagaaagc ggcucgcucg cagaaaagcc      360

cggcugaacc aucuuaagca ccugaucgcg aaugaguuua aacucaacua cgaagauuau      420

cagucuuucg acgagagccu ggccaaggcu uacaaaggcu cccugaucuc uccuuaugag      480

cuccgcuuua gagcccugaa ugaacuucug agcaagcaag auuucgcucg ggugauucuc      540

cauaucgcca agcgcagagg guacgacgau auuaaaaacu ccgacgauaa ggagaaagga      600

gcgauccuga aggccauuaa acagaaugaa gagaaacuug cuaacuauca gagcgucggc      660

gaguaccugu auaaagaaua cuuucagaag uucaaggaaa auucuaaaga auuuacgaac      720

gugcggaaua agaaagaguc cuacgaacgc uguaucgccc agagcuuccu caaggacgag      780

cugaaacuga uuuuuaagaa gcaaagagag uucggcuuuu cuuucuccaa aaaguucgaa      840

gaggaaguuc ucagcguggc uuuuuauaaa cgggcccuga aggauuucuc ucaccuuguc      900

gggaacugcu ccuuuuucac cgacgagaaa agagccccca agaauagccc ucuggcuuuu      960

auguucgugg cgcucacacg caucaucaac cugcuuaaua accugaagaa caccgaaggc     1020

auucucuaca caaaagauga ccugaaugcc cugcucaacg agguucugaa gaauggaacc     1080

cuuacauaua aacagaccaa gaaacugcuc gggcuguccg acgauuacga guuuaaggga     1140

gaaaagggca cguauuucau cgaguuuaaa aaguacaaag aguucauuaa ggcucuuggg     1200

gagcauaacc ugucucagga cgaucucaau gaaaucgcca aagacauuac ccugaucaag     1260

gaugagauca aacugaagaa ggcccucgcu aaauaugacc ugaaccaaaa ucagauugau     1320

agccuuucca agcuggaguu uaaagaccac cucaacauca gcuucaaggc ccugaaacug     1380

gugacacccc uuaugcucga aggaaagaag uacgaugagg cguguaauga acugaaccug     1440

aaagucgcua uuaaugagga caagaaagau uuucucccug ccuucaacga gaccuacuau     1500

aaggacgaag ugacaaaucc ggugguccug cgggccauca aagaguacag aaagguucuu     1560

aacgcucugc ucaagaaaua uggcaaggug cacaaaauua auaucgaacu ggcccgcgag     1620

gugggcaaga accauucuca gcgggcuaaa aucgaaaagg agcagaacga gaauuacaag     1680

gccaaaaagg acgcggaacu ggagugcgaa aaacuugggc ucaagauuaa cuccaaaaau     1740

auccugaagc ugagacucuu caaagagcaa aaggaauuuu gugccuauag cggagagaag     1800

auuaaaaucu cugaucugca ggacgagaag augcuugaaa uugaucacau cuaccccuau     1860

agccgcuccu ucgacgauuc uuacaugaac aaaguccugg uguuuaccaa gcagaaucaa     1920

gagaaacuca accagacgcc uuucgaggcu uuuggcaaug acagcgccaa guggcagaag     1980

auugaaguuc uggcuaaaaa ccugcccaca aagaaacaga agcggauccu ugauaaaaau     2040

uacaaggaca aggaacaaaa gaacuucaag gauagaaauc ucaacgacac ccgcuauauc     2100

gcccggcugg ugcugaauua cacaaaagau uaucucgacu uucugccucu uuccgaugac     2160

gagaacacca agcugaauga cacccagaaa gggagcaagg uccaugugga ggcgaagucu     2220

ggaaugcuca cauccgcccu gagacacacg uggggcuuca gcgcuaaaga ucgcaacaac     2280

caucugcacc acgccauuga cgcuguuauc auugccuacg ccaauaacuc uaucgugaag     2340

gcuuuuuccg auuucaaaaa ggaacaggag agcaauuccg cggaacuuua ugccaaaaag     2400

auuucugagc ucgacuacaa gaacaaacgg aaguuuuucg agcccuuuag cggguucaga     2460

cagaaagucc uggauaagau cgacgaaauc uucgugucca aaccggagcg gaagaaaccu     2520

ucuggagcuc ugcaugaaga gaccuuucgc aaggaagagg aguucuauca aagcuacggc     2580

ggcaaggaag ggguccucaa agcccuggag cuuggaaaga uuagaaaagu gaauggcaag     2640

aucguuaaaa acggggauau guuucgggug gacauuuuca agcacaagaa aacaaauaag     2700

uuuuacgccg uccccaucua uaccauggau uucgcucuga aagugcuccc uaacaaggcc     2760

guugcgagau ccaaaaaggg agaaauuaag gacuggaucc ugauggauga gaauuacgaa     2820

uuuugcuuca gccuguauaa agacucucuu auccugauuc agacaaagga uaugcaggag     2880

cccgaguuug uguacuauaa cgcuuucacc uccagcacgg ucucucucau cguguccaaa     2940

caugacaaua aguuugaaac acugagcaaa aaccagaaga uucucuucaa gaaugccaac     3000

gagaaagaag ugaucgccaa gucuauuggc auccaaaauc ugaaaguuuu cgagaaguac     3060

aucgucagcg cucuugggga agugaccaaa gccgaguuuc gccagcggga ggacuucaag     3120

aaaaagagac ccgcggcuac caagaaagcc ggccaggcca agaaaaagaa auaaggacua     3180

gucccuguuc ccagagccca cuuuuuuuuc uuuuuuugaa auaaaauagc cugucuuuca     3240

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3300

aaaaaaaaag aauu                                                       3314


<210>  4823
<211>  3725
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4823
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccagcgac      180

cugguucucg gacuggauau uggcaucggg uccgugggag ugggcauucu uaacaagguc      240

accggggaga ucauucauaa aaauucucgg aucuucccgg cggcucaggc cgaaaacaau      300

cuggugcgca gaacaaaccg gcaaggacgc agacucgccc ggcgcaagaa gcacagacgg      360

guucgccuga auagacuuuu ugaggaaagc ggccugauca cggacuucac caaaauuucc      420

aucaaccuca aucccuacca gcugcgggug aagggccuga cagaugagcu cucuaacgag      480

gagcuguuua uugcucuuaa aaauaugguc aagcaucgcg ggaucagcua ucuggacgau      540

gccuccgacg auggaaacuc uagcgugggc gacuacgcuc agauuguuaa agaaaauucc      600

aagcagcucg aaaccaagac accugggcaa auccagcugg agagauauca gaccuacgga      660

cagcuucggg gcgauuucac aguggaaaaa gacgggaaga aacaccgccu gauuaacguc      720

uuucccacca gcgccuauag aucugaggcg cuccggaucc ugcaaacgca gcaggaguuc      780

aauccucaga ucaccgauga auuuauuaac agauaccugg agauccucac aggaaagcgc      840

aaauauuacc auggccccgg caaugaaaag ucccggaccg acuacgggag auaucgcaca      900

agcggagaga cccuggauaa cauuuucggc auccuuauug ggaaaugcac guuuuacccu      960

gacgaguucc gggccgcuaa ggccucuuau acagcucaag aauuuaaccu gcucaaugac     1020

cugaacaauc uuaccgugcc gacagagacc aagaaacugu ccaaggaaca gaaaaaccag     1080

aucaucaauu acgugaagaa cgagaaagcc augggacccg cgaaacucuu caaguauauu     1140

gccaaacugc ugagcuguga ugucgcugac aucaagggcu acagaauuga uaaaucuggg     1200

aaggccgaaa uccacaccuu ugaggcuuau cgcaaaauga agacacucga aacgcuggac     1260

auugagcaaa uggaucggga gacccuugac aagcuggccu acguucucac acugaauacc     1320

gaaagagagg gaauccagga agcccuugag cacgaguucg cugauggcuc cuucagccag     1380

aaacaggugg acgaacuggu gcaauuucgc aaggcgaacu ccucuaucuu cggcaaaggg     1440

uggcauaauu uuagcgucaa gcucaugaug gagcugauuc cugaacugua cgagacaucc     1500

gaggaacaga ugaccauccu cacgcggcug ggaaaacaga agacaaccuc uucuuccaac     1560

aagaccaaau auauugauga gaaacuucug acagaagaga ucuacaaucc cgugguugcc     1620

aagagcguga gacaggcuau uaagaucguc aacgccgcca uuaaagaaua uggcgacuuc     1680

gauaauaucg ugaucgagau ggcucgcgaa accaacgagg acgacgagaa gaaagccauu     1740

caaaagaucc agaaggcgaa uaaagaugaa aaggacgcug ccaugcucaa agccgcuaac     1800

caguacaacg ggaaggccga gcugccucac ucuguuuuuc auggacacaa acagcuggcu     1860

acaaagauuc ggcuuuggca ccaacagggc gaaagaugcc ucuauacggg gaagaccauc     1920

agcauucaug aucugaucaa uaacuccaau caguucgagg uggaccacau ccugccccuc     1980

ucuauuacau uugaugacag ccuggccaac aaaguccuug uguacgcgac cgccaaucag     2040

gaaaagggcc aacggacacc guaucaggcu cuggauucca uggacgaugc cuggagcuuc     2100

cgcgagcuca aggcuuuugu cagagagucu aagacccugu ccaacaaaaa gaaggaauac     2160

cugcuuacgg aggaagacau cagcaaauuc gaugugcgga agaaauuuau ugagagaaau     2220

cucguugaca cccgcuacgc cucucgggug guccugaacg cgcugcagga acauuucaga     2280

gcccacaaga ucgauacaaa aguguccguu gugcgcggac aauuuaccag ccagcucaga     2340

cggcauuggg gcauugagaa gacacgcgac accuaucacc accaugcugu cgacgcccug     2400

aucaucgcug ccuccucuca gcuuaaucug uggaagaaac agaagaacac gcucgugagc     2460

uacuccgagg aucaacugcu ggacauugaa acaggggagc uuaucucuga ugacgaauau     2520

aaagagagcg uguucaaggc cccuuaccag cacuucguug auacccucaa auccaaggag     2580

uuugaagaca gcauucuguu cucuuaucag gucgauucca aauuuaauag aaagaucagc     2640

gacgcuacaa uuuacgcgac ccggcaggcc aaggugggaa aagauaaggc ugacgagacc     2700

uaugugcugg gcaaaaucaa ggauaucuac acacaagacg gguacgaugc cuucaugaaa     2760

auuuauaaga aggacaaauc uaaguuucuc auguaccgcc augaccccca gacguucgaa     2820

aaagucaucg agccuauucu ggaaaacuau cccaauaagc agaucaacga gaaaggaaag     2880

gaggugcccu guaauccuuu ucuuaaguac aaagaggaac acggcuauau uagaaaguac     2940

uccaaaaagg ggaacggccc ggaaaucaaa agccugaagu auuacgauuc uaagcucgga     3000

aaccacauug acaucacccc caaagauagc aauaacaagg uugugcugca guccgucagc     3060

ccuuggcggg ccgacgugua cuucaauaaa acaaccggca aguaugagau ccuggggcuu     3120

aaauacgcug aucuccaauu ugaaaaggga acaggcaccu auaagauuuc ucaggagaaa     3180

uacaacgaca ucaagaaaaa ggaggggguu gauuccgaca gcgaguucaa auuuacgcug     3240

uauaagaaug aucugcuccu ggugaaagac acagaaacca aggaacagca acuuuuccgc     3300

uuucugucca gaaccaugcc caagcagaaa cauuacgucg agcucaagcc uuaugauaaa     3360

cagaaguucg aaggaggcga ggcccugauu aaagugcugg ggaacguugc gaauagcggc     3420

cagugcaaga agggacuugg caaaagcaac aucuccauuu acaaggugcg gacagacguc     3480

cuggggaauc aacacaucau caaaaacgag ggagacaagc ccaaacucga uuucaagcgc     3540

ccggcugcca ccaagaaagc cggccaggcu aagaaaaaga aauaaggacu agucccuguu     3600

cccagagccc acuuuuuuuu cuuuuuuuga aauaaaauag ccugucuuuc agaucuaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3720

gaauu                                                                 3725


<210>  4824
<211>  4283
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4824
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccacccag      180

uucgagggau uuacaaaccu guaccaaguu agcaagacgc uccgguucga acugauuccg      240

cagggcaaaa cccuuaagca uauccaggag cagggguuua uugaagagga caaggcgcgc      300

aaugaucacu auaaagaacu gaagcccauc auugacagaa ucuacaaaac auaugcugau      360

caaugccucc agcuggugca gcuugacugg gagaaccugu ccgccgccau cgauucuuac      420

cggaaggaga aaaccgaaga gacacgcaau gcucucauug aagaacaggc caccuauaga      480

aacgcuaucc augacuacuu cauuggacgg acagauaauc ugaccgacgc caucaacaag      540

cgccacgcgg agauuuauaa gggccuguuu aaagccgaac ucuucaaugg gaaggugcug      600

aaacaacuug gaacggucac cacaaccgag caugaaaacg cucugcucag auccuuugau      660

aaguucacaa ccuacuuuag cggcuucuac gagaaucgga aaaacguguu uucugccgaa      720

gacaucagca cggcuauucc ucaccgcauc guucaggaua auuuccccaa guuuaaagag      780

aacugucaca ucuucacaag acugauuacc gccgugccuu cccuucggga gcauuucgaa      840

aaugucaaga aggcgaucgg cauuuuugug ucuacaagca ucgaggaagu uuucuccuuu      900

cccuucuaua accagcugcu cacccagacc caaauugacc uguacaauca gcugcucggg      960

ggaaucagcc gcgaggccgg cacagagaaa aucaaggggc ugaacgaggu gcuuaaccug     1020

gcuauucaga aaaaugauga gacggcccac aucauugcuu cucucccuca uagauuuauc     1080

ccgcuguuca agcaaauucu uuccgaccgg aacacccuga gcuuuauccu cgaagaguuc     1140

aaaucugacg aagaggucau ccaguccuuu ugcaaguaua agacacugcu gcgcaaugaa     1200

aacgugcucg agaccgccga ggcccuguuc aaugaacuua acagcauuga ucugacacac     1260

aucuuuauuu cucacaaaaa gcucgagacc aucuccagcg cucuguguga ccauugggau     1320

acgcuuagaa augcgcugua cgaacggaga auuuccgagc ucacaggaaa aaucaccaag     1380

ucugccaaag agaaggugca gcgcagccug aagcacgaag acauuaaccu gcaggagauc     1440

aucuccgcug ccggcaaaga acucagcgag gccuucaagc agaaaaccuc ugaaauucug     1500

ucccaugcuc acgccgcgcu ugaucaaccc cugccuacaa cccucaagaa acaggaggag     1560

aaggaaaucc uuaagagcca gcuggacucu cuucucgggc uguaucaucu gcucgauugg     1620

uuugcugucg acgagagcaa ugaaguugau cccgaguucu ccgcccggcu gacaggaauu     1680

aaacuugaaa uggagccuuc ucugagcuuc uacaacaagg ccagaaauua ugcuacgaaa     1740

aagcccuacu ccguggagaa auuuaagcuc aacuuccaaa ugccgacccu ggccagcggc     1800

ugggacguga auaaagaaaa gaacaauggc gcuauccugu uugucaagaa cgggcuuuac     1860

uaucucggaa uuaugccuaa acagaagggc cgcuacaaag cccugucuuu cgagcccaca     1920

gaaaagaccu ccgagggguu ugauaaaaug uauuacgacu auuucccuga cgcggccaag     1980

augaucccca agugcagcac acagcugaaa gcugugaccg cccacuuuca gacgcacacc     2040

acacccaucc uccugucuaa caauuucauu gaaccucuug agaucaccaa ggagauuuac     2100

gaucugaaca auccggaaaa agagcccaag aaauuucaaa cagcuuaugc caagaagacc     2160

ggagaccaga aaggcuaccg ggaagcgcuc uguaagugga ucgauuucac gagagacuuu     2220

cuguccaaau acacaaagac cacaagcauu gaucuguccu cucuucgccc uagcucccag     2280

uauaaagacc ucggggagua cuaugccgag cugaaccccc ugcucuacca uaucucuuuc     2340

cagcggaucg cugaaaagga gauuauggau gccguugaaa ccggaaagcu guaucuuuuu     2400

caaaucuaca auaaagacuu cgcuaagggc caccauggca aaccuaaccu gcacacccuc     2460

uauuggacag ggcuguucag ccccgagaau cuggccaaga cguccauuaa acuuaacgga     2520

caggccgaac ucuuuuacag accuaagagc cgcaugaaac ggauggcuca cagacugggc     2580

gagaagaugc ugaauaagaa acucaaggau cagaaaaccc cgauccccga cacacuguac     2640

caagagcuuu augauuacgu gaaccaucgc cugucucacg accucuccga ugaagcgcgg     2700

gcccugcugc cuaaugucau uaccaaggag gugagccaug aaaucaucaa agacagacgg     2760

uucacaucug acaaguuuuu cuuucacguu cccauuaccc uuaacuauca ggcugccaau     2820

uccccuagca aguucaacca gcgcgugaau gccuaccuga aagagcaucc cgaaacgccg     2880

aucauuggga ucgauagagg agagcggaac cucauuuaua ucacagucau ugacucuacc     2940

ggcaagaucc uggagcagag aagccucaac accauccaac aguuugauua ccagaaaaag     3000

cuggacaauc gcgaaaaaga gcggguggcu gccagacagg cgugguccgu cguggggaca     3060

auuaaggauc uuaagcaagg cuaucugagc cagguuaucc acgaaauugu ggaccucaug     3120

auccacuacc aggcugucgu gguucuggag aaccugaauu ucggauuuaa aucuaagcgc     3180

accggcauug ccgaaaaagc cguguaccag caauucgaga agaugcuuau cgauaaacug     3240

aacugccucg uccugaagga cuauccugcu gagaagguug ggggagugcu caaucccuac     3300

cagcugacag aucaguuuac guccuucgcc aaaaugggca cccaaagcgg guuccuguuu     3360

uaugugcccg cgccuuacac auccaagauc gacccgcuga ccggauucgu cgaucccuuu     3420

guguggaaaa caauuaagaa ccaugaaucu agaaaacacu uccucgaggg cuuugacuuc     3480

cugcauuaug augugaagac cggggacuuu auccugcacu ucaagaugaa ucggaaccuu     3540

agcuuucagc gcggccugcc uggauucaug cccgcuuggg acauugucuu ugagaaaaau     3600

gaaacgcagu ucgaugccaa gggcaccccu uuuaucgccg ggaaaagaau ugugcccguu     3660

aucgagaacc aucgguucac aggacgcuac agagaccucu auccugcuaa ugaacugauc     3720

gcccuccugg aggaaaaggg cauuguguuc cgggaugggu ccaacauccu ucccaaacug     3780

cuggagaaug acgauucuca cgcuauugac accauggucg cccucauccg cagcgugcug     3840

cagaugagaa acuccaaugc ggccacagga gaagauuaca uuaacucucc gguucgggac     3900

cuuaacggcg uguguuuuga uagccgcuuc caaaauccug aguggcccau ggacgcugau     3960

gccaacgggg cuuaccacau cgcccugaag ggccagcucc ugcucaauca ucugaaagag     4020

uccaaggacc uuaagcugca gaacggaauu agcaaucagg acuggcuggc cuauauccaa     4080

gaacucagaa acaaacggcc ugcugcgacc aagaaagccg gccaggcuaa gaaaaagaag     4140

uaaggacuag ucccuguucc cagagcccac uuuuuuuucu uuuuuugaaa uaaaauagcc     4200

ugucuuucag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaaaaaaaa aaaaaaaaga auu                                             4283


<210>  4825
<211>  4262
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4825
gggagacucc ugucgggcgg aaguaggagg aguagaguuu aaaaacagua cucuuuuucc       60

gguucgggac guaguugaag caacgacaag ccggauaacc gcucuugaga caggaagcuu      120

accauggccc ccaagaaaaa gagaaaagug ggcauccacg gggucccugc ugccagcauu      180

uaccaggagu ucguuaacaa guauucccug ucuaaaaccc uccgguuuga acugaucccg      240

caaggaaaga cacuugagaa uauuaaggcg cgcggccuga uccucgacga ugaaaagaga      300

gcuaaggacu acaaaaaggc caaacagauu aucgauaagu aucaucaguu cuuuaucgag      360

gaaauucuga gcuccgugug caucucugag gaccuucugc agaacuacag cgauguguau      420

uucaagcuca aaaaguccga cgaugacaau cugcaaaaag auuuuaaguc ugccaaagac      480

acgauuaaga aacagaucag cgaguacauu aaggauuccg aaaaguucaa aaaccuguuu      540

aaucagaacc ucaucgacgc uaagaaaggg caggagagcg aucugauucu uuggcugaag      600

caaucuaaag acaauggaau cgaacucuuc aaggccaacu ccgacaucac cgauauugac      660

gaggcucugg agaucauuaa gagcuuuaaa ggcuggacaa ccuauuucaa gggguuucac      720

gaaaaucgga aaaacgucua cucuuccaau gauaucccca caagcauuau cuaccgcauc      780

guggacgaua accuuccuaa guuccuggag aauaaagcca aguaugaauc ucucaaggac      840

aaagcgcccg aggccauuaa cuacgaacag aucaagaaag aucuggcuga ggagcugacc      900

uuugacauug auuauaagac auccgaaguu aaucagagag uguucagccu cgacgagguc      960

uucgaaaucg ccaacuuuaa caauuaccug aaccaguccg gaauuaccaa auucaauacg     1020

aucaucggcg gcaaguuugu gaacggggag aauaccaagc ggaaaggaau uaacgaauau     1080

aucaaucuuu acucucaaca gauuaacgau aagacacuga aaaaguauaa aaugagcguu     1140

cucuucaagc agauccuguc cgacaccgag agcaaaucuu uugugauuga caaacuugag     1200

gaugacuccg augucgugac aaccaugcag agcuucuacg aacaaaucgc ugccuuuaag     1260

acgguggagg aaaaaucuau uaaggagaca cugagccucc uguucgacga ucugaaagcg     1320

cagaagcucg accuguccaa aaucuacuuu aagaaugaua agucucuuac cgaccugagc     1380

cagcaagucu ucgaugacua uuccguuauc ggcacagccg ugcucgaaua cauuacccag     1440

cagaucgcuc cuaaaaaccu ggauaauccc agcaagaaag agcaggagcu uauugccaag     1500

aaaaccgaaa aggcuaagua ucugucucuc gagacaauca aacuggcccu ggaagaguuu     1560

aacaagcauc gcgacauuga uaaacaaugu agauucgagg aaauccucgc caauuuugcu     1620

gcgaucccua ugauuuucga cgagaucgcc cagaacaagg acaaucuggc ucagauuucc     1680

aucaaauacc agaaccaagg gaagaaggau cuucugcagg ccagcgccga agacgaugug     1740

aaagcuauua aggaccuccu ggaucagacg aacaaucugc uucacaaacu caagaucuuc     1800

cauaucucuc aguccgagga caaagccaac auucuggaua aggacgaaca cuuuuaucug     1860

gucuucgagg agugcuacuu ugaacucgcg aauaucgugc cgcuguauaa caaaauucgg     1920

aauuacauca cccaaaagcc cuacagcgau gagaaguuca aacuuaacuu ugaaaauucc     1980

acacuggcua acggauggga caagaauaaa gagccugaua acaccgccau ucucuucauc     2040

aaggacgaua aauauuaccu gggcguuaug aauaagaaga acaauaaaau cuuugacgac     2100

aaggccauua aagaaaacaa gggggaggga uauaaaaaga ucguguacaa gcugcuuccc     2160

ggcgcuaaua aaaugcuccc uaaggucuuc uuuucugcca aaagcauuaa guucuauaac     2220

cccuccgagg auauccugcg cauuagaaac cacucuacac auaccaaaaa uggcagcccg     2280

cagaaggggu acgaaaaguu ugaguucaac aucgaagacu gucggaaauu uauugauuuc     2340

uauaagcagu ccaucagcaa acacccugag uggaaggacu ucggauuucg cuucucugau     2400

acgcaaagau acaauuccau cgacgaauuu uaccgggagg uggagaacca gggcuauaaa     2460

cugacauucg aaaauauuag cgagucuuac aucgauuccg uugugaacca ggggaagcuc     2520

uaucuguuuc agauuuacaa uaaggacuuc agcgcuuauu cuaaaggacg ccccaaccuu     2580

cauacccugu acuggaaggc ccucuuugau gaaagaaauc ugcaagacgu cguguauaaa     2640

cugaacggcg aggcggagcu uuucuaccgg aagcagagca ucccuaaaaa gauuacccac     2700

cccgccaaag aagcuaucgc caauaagaac aaggauaauc ccaaaaagga guccgucuuu     2760

gaauacgacc ucaucaaaga caagagauuc acagaggaua aauuuuucuu ccacugcccu     2820

auuaccauca acuuuaagag cucuggggcu aauaaguuca acgacgaaau uaaccugcug     2880

cucaaagaga aggccaauga ugugcauauc cuguccauug accgcggaga gcggcaccuu     2940

gcguauuaca cacugguuga uggcaaaggc aacaucauca agcaggacac guuuaauauu     3000

aucgggaacg auagaaugaa aaccaauuau caugacaagc ucgccgcuau ugaaaaggau     3060

cgcgacagcg cccggaaaga uuggaagaaa aucaacaaua uuaaggagau gaaagaagga     3120

uaccuguccc agguggucca cgagaucgcu aagcugguga uugaauauaa cgccaucguu     3180

guguucgagg accuuaauuu uggcuucaag agagggcgcu uuaaagucga gaagcaagug     3240

uaccagaaac ucgaaaagau gcugaucgag aaacugaacu aucucguguu caaggauaau     3300

gaauuugaca aaacaggagg cguucugcgg gccuaccagc uuaccgcucc guucgagaca     3360

uuuaagaaga uggggaaaca gaccggcauu aucuacuaug uccccgcggg auucacgucu     3420

aagauuuguc cugugaccgg cuuugugaac caacuguacc ccaaauauga aagcgucucc     3480

aagucucagg aguucuucag caaauuugac aagaucugcu acaaucucga uaagggguau     3540

uucgaguuuu ccuucgacua caaaaacuuu ggagauaagg ccgcuaaagg caaguggaca     3600

auugccucuu ucgggagcag acugaucaau uuucgcaacu ccgacaaaaa ucauaacugg     3660

gauacccggg aaguguaucc uacaaaggag cuggaaaaac uucugaaaga cuacagcauc     3720

gaguacggac acggcgagug uauuaaggcc gcuaucugcg gggaaucuga uaaaaaguuc     3780

uuugccaaac ucaccuccgu ucugaacacg auucuccaga ugagaaauag caagacaggc     3840

accgagcugg acuaucuuau cucucccgug gcggauguca acggaaauuu cuuugacucc     3900

cgccaagcuc cgaagaacau gccucaggau gccgacgcca auggcgcuua ccacauuggg     3960

cugaaaggac ucaugcugcu gggccggauc aagaacaauc aggaagggaa aaagcugaac     4020

cuugugauca aaaaugagga auauuucgag uuuguucaga acagaaauaa caagcggccc     4080

gccgcgacaa agaaggcugg acaagccaag aaaaagaaau aaggacuagu cccuguuccc     4140

agagcccacu uuuuuuucuu uuuuugaaau aaaauagccu gucuuucaga ucuaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa     4260

uu                                                                    4262


<210>  4826
<211>  4452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4826
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccgac aagaaauaca gcauuggacu ggauaucggc      180

accaacuccg uugggugggc ggugauuaca gacgaguaua aggugccguc uaagaaauuc      240

aagguccucg gaaauacgga ucggcauagc aucaaaaaga accugauugg cgcucugcug      300

uuugacuccg gggaaaccgc cgaggccaca cgccucaaaa gaaccgcucg gcgcagauac      360

acacggcgca agaauagaau cugcuaucug caggaaaucu ucucuaacga gauggccaag      420

guggaugaca gcuuuuucca ccggcuugaa gaguccuuuc ugguugagga agauaaaaag      480

caugagcgcc accccauuuu cggaaauauc guggacgaag ucgcuuacca ugagaaauau      540

ccuaccauuu accaccucag aaagaaacug guggauucua cagacaaggc cgaucugcgg      600

cucaucuauc uggcgcuugc ccacaugauu aaauuucgcg gccauuuccu gaucgagggc      660

gaccucaacc ccgauaauag cgacguugac aagcuguuua uucaacuugu gcagaccuac      720

aaccagcugu ucgaagagaa uccuaucaac gcuuccgggg ucgaugccaa ggcuauccuc      780

agcgccagac ugucuaaauc ccggcgccug gaaaaucuca uugcgcagcu gcccggagag      840

aagaaaaacg gccuuuuugg gaaucugauc gcccucagcc ugggacuuac gccuaacuuc      900

aagucuaauu uugaccuggc ugaagaugcc aaacuccaac uguccaagga caccuacgau      960

gacgaucugg acaaccuccu ggcucagauu ggcgaucagu augccgaccu uuuccuggcc     1020

gcuaagaacc ucagcgaugc gauccugcuu ucugacauuc ugagagugaa uacagagauc     1080

accaaagccc cgcucuccgc uagcaugauc aagcgguacg acgagcacca ucaggaucug     1140

acacugcuca aagcccuggu gagacaacag cuucccgaaa aguauaaaga gauuuucuuu     1200

gaccagucca agaacgggua cgccggauau aucgauggcg gcgcuucuca ggaagaguuc     1260

uacaaguuua uuaaaccuau ccuggaaaag auggacggga ccgaggagcu ccuggucaaa     1320

cugaaucgcg aagaucuucu ccggaagcaa agaacguucg acaacggaag cauuccccac     1380

cagauccacc ugggcgagcu gcaugccauc cuccgccggc aggaagauuu uuauccuuuc     1440

cugaaagaca auagagagaa gauugaaaag auccuuacau uucgcauucc cuacuacguu     1500

gggccgcugg cgcggggaaa cuccagauuc gcuuggauga cccgcaaaag cgaggagaca     1560

aucaccccuu ggaauuuuga agagguggug gauaagggcg ccucugccca auccuucauu     1620

gaacggauga ccaacuuuga caaaaaucuc cccaacgaga agguccugcc uaaacacagc     1680

cugcuuuaug aguacuucac aguguauaau gaacucacga agguuaaaua cgugaccgag     1740

gggaugagaa agcccgcuuu ucugucugga gaacagaaga aagccaucgu cgaucuucuc     1800

uucaagacaa accgcaaagu gaccguuaag cagcugaaag aggacuauuu caagaagauu     1860

gaauguuuug auagcgugga gaucuccggc gucgaggacc gguucaaugc uucucuuggc     1920

acauaccaug accugcucaa aaucauuaag gauaaagacu uucuggauaa cgaagagaau     1980

gaagacaucc uggaggauau ugugcuuacc cucacgcugu ucgaagacag agagaugauc     2040

gaggaacggc ugaagacaua ugcccaccuc uuugaugaca aagucaugaa gcagcugaag     2100

cgcagacggu acaccgggug gggaagacuu agccgcaaac ugauuaacgg cauccgggau     2160

aagcaauccg ggaaaaccau ccucgacuuc cuuaagagcg auggauuugc gaacagaaau     2220

uucaugcagc ugauucauga cgauucucug acauuuaaag aggacaucca gaaggcccag     2280

guguccggcc aaggggacag ccuccacgaa cacauugcua accuggccgg cucucccgcu     2340

aucaagaaag gcauucugca gaccguuaag guggucgaug agcucgugaa aguuauggga     2400

cgccauaagc cugaaaauau cgugaucgag auggccagag agaaccagac aacgcagaaa     2460

gggcaaaaga acucccggga acgcaugaaa agaauugagg aaggaaucaa ggagcugggc     2520

agccagauuc uuaaggagca cccggucgaa aauacccagc ugcagaauga gaaacucuac     2580

cuguauuacc ugcaaaacgg gcgggacaug uauguggauc aggaacuuga caucaaucgc     2640

cuguccgauu acgacgugga ucauauuguu ccccagucuu uccucaagga cgauagcauc     2700

gacaacaaag uccugacaag auccgauaag aaucggggaa aaucugacaa cgugccuagc     2760

gaggaagugg ucaagaagau gaaaaauuau uggcgccaac uccugaacgc gaaacuuauc     2820

acccagagaa aauuugacaa ucugacaaag gccgagcggg gcgggcucuc cgagcuggau     2880

aaagcuggcu ucauuaagcg ccagcuggug gaaaccagac agaucacgaa gcacguugcc     2940

caaauucuug acagccggau gaacaccaaa uacgaugaga acgacaagcu gaucagagaa     3000

gugaaaguca uuacacucaa gucuaaacug guguccgauu uucgcaagga cuuccaguuc     3060

uauaagguuc gggagaucaa uaacuaccac caugcucacg augccuaccu caaugccgug     3120

gucggaaccg cucugauuaa aaaguauccc aaacuugaaa gcgaguuugu guacggcgac     3180

uauaagguuu acgaugugag aaaaaugauc gcgaagucug agcaggaaau cgggaaggcc     3240

acagcuaaau auuucuuuua cuccaacauu augaauuucu uuaagaccga gaucacgcug     3300

gccaacggag aaauucgcaa acggccucuc aucgagacaa auggcgaaac cggggagauu     3360

gucugggaca agggaagaga uuucgccaca gugcgcaaag ugcugagcau gccccagguc     3420

aacaucguua agaaaaccga ggugcaaacc ggcggguuuu cuaaggaaag cauccugccg     3480

aagagaaauu ccgacaaacu uauugcucgg aagaaagauu gggacccuaa gaaauauggc     3540

ggauucgaca gccccacagu ggccuacucu guccuggugg uugcgaaggu ggagaagggc     3600

aaauccaaga aacucaagag cgucaaagaa cugcucggga ucacgauuau ggagcgcucc     3660

ucuuuugaga agaacccuau cgauuuccug gaagcuaagg gauacaaaga ggugaagaaa     3720

gaccuuauua ucaagcugcc caaauauagc cuguuugaac ucgagaaugg ccggaagaga     3780

augcuggccu ccgccgggga acuucagaag ggaaacgagc uggcucuccc uucuaaauac     3840

guuaauuucc uguaucucgc cagccauuac gagaagcuga aaggcucccc cgaagauaac     3900

gagcagaagc agcuuuucgu ggaacaacac aaacauuauc uggacgagau cauugaacag     3960

aucucugagu uuagcaagcg ggucauucug gcggaugcua aucucgacaa agugcugucc     4020

gccuacaaca agcaccgcga uaagccgauc agagagcagg ccgaaaacau uauccaccuu     4080

uucacccuga caaaucucgg ggcuccugcc gcuuuuaaau auuucgacac cacaauugau     4140

cggaagcgcu acaccagcac gaaagagguu cuggacgcca cacucaucca ucaaucuauc     4200

accggccugu acgaaaccag aauugaucuu ucccagcugg gaggcgacaa gcggcccgcg     4260

gccacaaaga aggcugggca ggccaagaaa aagaaauaag gacuaguguc caccuguccc     4320

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     4380

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4440

aaaaaaagaa uu                                                         4452


<210>  4827
<211>  5235
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4827
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaac uucaagauuc ugccgaucgc gauugaccuc      180

ggaguuaaaa auacaggcgu guuuagcgcu uucuaccaga aggggacauc ccuggagcgg      240

cuugauaaca agaauggaaa aguguaugaa cugucuaagg acagcuacac gcuccugaug      300

aacaaucgca ccgccagacg gcaucaacgc agaggcaucg aucggaaaca gcuugucaag      360

cgccuguuua aacucauuug gacagagcag cugaaccugg aaugggacaa ggauacccag      420

caagccaucu ccuuccucuu uaauagacgg ggguucucuu uuaucacaga cggauauagc      480

cccgaguacc ugaacauugu gccugaacag guuaaggcua uccuuaugga uauuuucgac      540

gauuauaaug gcgaggacga ucuggacucc uaccucaaac uggccaccga gcaggaaucu      600

aagaucagcg agauuuauaa caaacuuaug cagaagaucc uggaauuuaa acucaugaag      660

cugugcacag auauuaaaga cgacaaggug uccaccaaga cgcugaaaga gaucaccagc      720

uacgaguucg aacuccuggc ugauuaccuu gccaauuauu cugagucccu gaagacacaa      780

aaauuuagcu acaccgacaa gcagggcaac cucaaagaac ugucuuauua ccaccaugau      840

aaguauaaua uccaggaguu ccuuaagcgc cacgcgacaa uuaacgacag aauccuggau      900

acccuccuga cggacgaucu ggacauuugg aauuuuaacu ucgaaaaauu cgauuuugac      960

aagaacgagg agaaacucca gaaucaagaa gauaaggacc auauccaggc ccaucugcac     1020

cacuucgucu uugcugugaa caaaauuaag uccgagaugg ccagcggggg acggcaccgc     1080

ucucaguacu uccaagaaau cacaaauguu cuugacgaga acaaucauca ggaaggcuau     1140

cugaagaacu uuugugagaa ucuccacaac aaaaaguacu ccaaucugag cgugaaaaac     1200

cuugucaauc ugaucgggaa ccucuccaau cuggagcuga agccccucag aaaauacuuc     1260

aacgauaaga uucacgcuaa ggccgaccau ugggaugaac agaaauuuac cgagacauau     1320

ugccacugga uccugggaga auggcgggug ggcgugaagg accaggauaa aaaggacggg     1380

gcgaaauacu cuuauaagga ucuuuguaau gagcugaaac aaaaggucac caaggccgga     1440

cucguugacu uccugcugga acuugauccu ugccgcacca uucccccuua ccucgacaac     1500

aacaauagaa aaccgcccaa gugucagagc cugauccuga acccuaaauu ucucgauaau     1560

caguauccca acuggcagca auaccugcag gagcuuaaga aacugcaguc cauucagaau     1620

uaucucgaca gcuucgagac agaucugaag gugcugaagu cuuccaaaga ccaaccuuac     1680

uuuguggaau acaagagcuc uaaccagcag aucgcuagcg gccagcggga cuauaaagau     1740

cuugacgcca gaauccucca auucauuuuu gaucgcguca aggcuuccga cgagcugcug     1800

cucaaugaaa ucuacuucca ggccaaaaag cugaagcaga aagccucuag cgagcuugag     1860

aagcuggaau ccagcaaaaa gcucgaugag gugauugcua acucucaacu gucccagauc     1920

cugaaaagcc agcauacgaa uggcauuuuc gaacagggga ccuuucuuca ccucguuugc     1980

aaguauuaca agcaacggca gagagcgcgc gacucucggc uguauaucau gcccgaguac     2040

agauaugaua aaaagcugca uaaauacaac aauacaggac gcuucgacga ugacaaccag     2100

cuccugaccu acuguaauca caagccgcgg cagaaaagau aucaacuucu gaacgaucuc     2160

gccggcgugc ugcaggucuc cccuaauuuu cugaaggaca aaauugggag cgaugacgac     2220

cuuuucaucu ccaaguggcu cguggaacac auccgcggau uuaagaaggc uugcgaggau     2280

ucucugaaaa uucagaaaga caaccggggc cugcucaacc auaagaucaa uauugccaga     2340

aacacaaaag ggaaguguga gaaggaaauc uucaaucuga uuugcaaaau cgagggaagc     2400

gaagauaaga aaggcaacua caagcacggc cuugccuaug agcugggggu ucuccuguuu     2460

ggagaaccca augaggcuuc caaaccugag uucgaccgca agaucaagaa auuuaacucu     2520

auuuacagcu ucgcccagau ccaacagauu gcguuugcug aacggaaggg caaugccaac     2580

accugugccg ugugcuccgc ugauaaugcc cauagaaugc agcaaaucaa aauuacggag     2640

cccgucgaag acaacaagga uaaaaucauc cugagcgcua aggcccagcg gcuucccgcg     2700

auuccuacac gcaucgugga cggggccguc aagaaaaugg cuaccauucu ggccaagaau     2760

aucguggaug acaacuggca gaauauuaaa cagguucucu cugcuaagca ccaacugcac     2820

aucccgauca uuaccgaguc caacgccuuc gaauuugagc ccgcgcucgc cgaugugaaa     2880

ggaaagagcc ugaaggacag acggaaaaag gcucuugaga gaaucucucc ugaaaauauu     2940

uucaaagaca agaacaaccg caucaaagag uucgccaagg gcauuuccgc uuauagcggg     3000

gccaaucuga cagauggcga cuuugaugga gccaaagagg aacucgacca uaucauuccc     3060

cggucucaca agaaguacgg cacccugaac gaugaggcua aucugaucug cgucacaaga     3120

ggggacaaca aaaauaaggg aaaccgcauc uucugucuua gagaucuggc ggacaauuau     3180

aaacucaagc aguuugaaac gaccgaugac cuggagauug agaaaaagau cgccgauaca     3240

auuugggacg cuaacaagaa agauuucaag uuuggcaauu accggagcuu caucaaccuc     3300

accccucagg aacagaaagc cuuucgccau gcccuguucc uugcugacga aaaucccauu     3360

aagcaagccg ugaucagagc gaucaacaau cggaaccgca cauuuguuaa ugggacccag     3420

agauacuucg cugaggugcu ggccaacaau auuuaucucc gggccaaaaa ggagaaccug     3480

aacacggaca agaucuccuu ugauuacuuc ggcauuccga ccaucggaaa ugggcgcggc     3540

auugcugaaa ucagacagcu guaugagaaa gucgacagcg auauccaggc cuacgcgaag     3600

ggagacaaac cucaggcuuc uuauucccac cuuauugaug ccaugcuggc cuucugcauc     3660

gcugccgacg aacaucggaa cgauggcagc auugggcucg agaucgacaa gaauuacucc     3720

cuguaucccc ucgauaaaaa cacaggagaa guguuuacca aggacauuuu cucucaaauc     3780

aagauuacag auaaugaguu uagcgacaaa aagcuggugc gcaaaaaggc uaucgagggc     3840

uucaacaccc acagacagau gacgcgggau gggaucuacg ccgaaaauua ccuuccuauu     3900

cugauccaca aagagcugaa cgaaguuaga aagggauaua cauggaaaaa uuccgaggaa     3960

auuaagaucu uuaagggcaa aaaguacgac auucagcaac ucaacaaucu ggucuauugu     4020

cuuaaauucg uggacaagcc caucucuauc gauauucaga ucagcacccu ggaggagcuc     4080

cgcaacauuc ugacaaccaa uaacaucgcg gccaccgcug aauacuauua cauuaaucuc     4140

aaaacacaga agcugcauga guauuacauc gaaaacuaca acacggcccu uggguauaag     4200

aaauacucca aggagaugga guuucugcgg ucucuggcuu auagaagcga acgcgugaaa     4260

aucaagucca uugacgaugu caaacaagug cucgacaagg auagcaauuu caucauuggc     4320

aagaucaccc ugccuuuuaa aaaggagugg cagcggcuuu acagagaaug gcagaacaca     4380

accauuaaag acgauuauga guuccugaag ucuuucuuca auguuaaauc caucacaaag     4440

cuccacaaga aagugcgcaa ggacuuuagc cugcccauuu cuaccaacga aggaaaauuc     4500

cucgucaagc ggaaaacgug ggauaauaac uucaucuacc agauccugaa ugacuccgau     4560

agcagagccg acggcacaaa gccguuuauu ccugccuucg acaucucuaa aaacgagauu     4620

guggaggcua ucauugauuc cuuuaccagc aagaauaucu ucuggcuucc caagaacauc     4680

gaacugcaaa aaguugacaa uaagaacauu uuugcgaucg auaccuccaa augguucgag     4740

guggaaacac ccagcgaccu gcgcgauauu gggaucgcca ccauucagua uaagaucgac     4800

aauaacucuc ggccuaaagu cagagugaag cucgauuacg uuaucgacga ugacagcaag     4860

auuaauuacu uuaugaacca uucccugcuu aaaucucggu auccggauaa ggugcuggag     4920

auccucaaac agagcacaau uaucgaauuu gaguccucug gauuuaacaa gacgauuaaa     4980

gagaugcugg gcaugaagcu cgcugggauc uacaaugaaa ccagcaacaa uaagcgcccc     5040

gccgccacca aaaaggcugg acaggccaaa aagaaaaagu aaggacuagu guccaccugu     5100

cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac     5160

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     5220

aaaaaaaaaa gaauu                                                      5235


<210>  4828
<211>  3507
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4828
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaag cggaacuaca uucugggacu cgacaucggc      180

auuaccagcg uuggguaugg aaucauugau uacgagacac gcgacgugau cgaugcgggc      240

gugagacugu ucaaagaagc uaaugucgag aacaaugaag ggcggcgcuc caagagagga      300

gcccggcgcc uuaagagacg gcgcagacau cggauccagc gcgugaaaaa gcugcucuuu      360

gacuauaacc ugcuuacgga ucacucugag cugagcggca uuaauccgua cgaagccaga      420

guuaaaggcc ucucccaaaa gcugucugag gaggaguuca gcgcugcccu gcuccaucug      480

gcuaaacggc gcggggugca caacgucaau gaaguggaag aggacaccgg aaacgagcuu      540

uccacaaagg aacagaucuc uagaaauagc aaggcccugg aggaaaaaua uguugcggag      600

cuccagcugg aacggcuuaa gaaagauggc gaggugagag gguccauuaa ccgcuuuaag      660

accagcgacu acgucaaaga ggccaagcag cugcucaaag ugcaaaaggc uuaucaucag      720

cuggaucagu cuuucaucga cacauacauu gaucugcucg aaacccggag aacauacuau      780

gagggacccg gcgaaggguc cccuuuugga uggaaggaca ucaaagagug guacgaaaug      840

cugaugggcc acugcaccua uuuccccgag gagcuucgca gcgugaagua cgccuauaau      900

gcugaucugu acaacgcccu caaugaccug aacaaucuug ucauuacgcg ggacgaaaac      960

gagaaacugg aauauuacga gaaguuucag aucaucgaaa acguuuucaa acaaaagaag     1020

aaaccuaccc ucaagcagau ugcgaaagag auccugguga augaggaaga uauuaagggc     1080

uacagaguga caucuaccgg gaaacccgag uuuacaaacc ugaaggucua ucacgacauc     1140

aaggauauua ccgcccgcaa agaaaucauc gagaaugcug agcuccugga ccagauugcc     1200

aagauccuua cgauuuacca guccagcgaa gauauccaag aggaacugac aaaccucaau     1260

ucugagcuga cccaggaaga gauugagcag aucuccaacc uuaaaggaua uacaggcacc     1320

cauaaucuga gccucaaggc uaucaaccug auucuggacg aacucuggca caccaaugau     1380

aaccaaaucg ccauuuucaa ucggcugaaa cuugugccua agaagguuga ccugucccag     1440

cagaaagaga ucccgacaac gcucguggau gacuuuauuc ugucucccgu cgugaagcgc     1500

agcuucaucc aguccauuaa aguuaucaac gccaucauua agaaauacgg gcugccuaau     1560

gauaucauua ucgaacuugc ucgcgagaag aacagcaaag acgcgcaaaa gaugauuaau     1620

gaaaugcaga agcggaacag acagaccaac gagcgcaucg aggaaaucau ucggacaacc     1680

ggaaaagaga augccaagua ucucaucgag aaaauuaagc ugcaugacau gcaggaaggc     1740

aaaugucugu acucucucga agcuaucccc cuggaggauc uucugaacaa uccuuuuaac     1800

uaugaggugg accacauuau ccccagaucc gucagcuucg auaauucuuu caacaauaag     1860

gugcucguca agcaagaaga gaacagcaaa aaggggaauc ggacaccguu ucaguaccug     1920

uccucuagcg acuccaaaau cagcuacgaa accuucaaga aacacauucu gaaccuugcc     1980

aagggaaagg gccgcaucuc uaaaacgaag aaagaguauc uccuggagga aagagauauu     2040

aaucgguuuu ccgugcagaa ggacuucauc aacagaaauc ugguugauac acgcuacgcc     2100

acccggggcc ucaugaaccu gcuuagaagc uauuuucgcg ugaauaaccu ggacgucaaa     2160

gugaagucua uuaacggggg auucaccucc uuucucagac ggaaguggaa auucaagaaa     2220

gagcgcaaua agggcuacaa acaucacgcu gaagaugccc ugaucaucgc gaacgcugac     2280

uuuauuuuca aggaguggaa aaagcuggau aaggccaaaa agguuaugga aaaucagaug     2340

uuugaggaga aacaagccga aagcaugccu gagaucgaaa cagagcagga auauaaggag     2400

auuuucauca caccccauca gauuaaacac aucaaggacu uuaaggauua caaauauucc     2460

cauagagugg acaagaaacc uaaccgggag cuuauuaaug acacccucua cucuacgcgc     2520

aaggaugaca aagggaacac ccugaucguc aauaaccuga auggacucua cgauaaggac     2580

aacgauaagc ugaagaaacu uaucaauaaa agccccgaaa agcugcucau guaucaccac     2640

gacccccaga cauaccaaaa acugaagcug auuauggagc aguauggcga ugaaaagaac     2700

ccucuuuaca aauauuacga ggaaaccggg aauuaucuca caaaguacuc caaaaaggac     2760

aacggcccgg ugaucaaaaa gauuaaguac uauggaaaua aacugaacgc ucaucuggau     2820

aucaccgacg auuaccccaa cucuagaaau aaggugguua aacucagccu gaagccuuau     2880

cgguucgacg ucuaccuuga uaacggcgug uauaaauucg ugacggucaa gaaucuggac     2940

gugauuaaaa aggagaacua cuaugagguu aauuccaagu gcuacgaaga ggccaaaaag     3000

cucaaaaaga ucagcaacca ggcugaauuu aucgccucuu ucuacaauaa cgaccugauu     3060

aaaaucaaug gggagcugua ucgcgugauu ggagucaaca augaucuucu gaacagaauc     3120

gaggugaaua ugauugacau caccuaccgg gaauaucucg agaacaugaa ugauaagaga     3180

cccccuagaa ucauuaagac aaucgcgucc aaaacccaaa gcauuaagaa auacucuaca     3240

gacauccugg gcaaccucua ugaaguuaag uccaaaaagc acccccagau uaucaagaaa     3300

gggaagcggc cggccgcuac caaaaaggcc ggacaggcua aaaagaagaa auaaggacua     3360

guguccaccu gucccuccug ggcugcugga uugucucguu uuccugccaa auaaacagga     3420

ucagcgcuuu acagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aagaauu                                         3507


<210>  4829
<211>  4575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4829
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcccug uucaacaagu gcauuaucau uagcaucaau      180

cucgacuuuu ccaacaaaga gaaguguaug accaagccgu acucuauugg acuggauauc      240

ggcacaaaua gcguugggug ggcggugauc acggacaacu auaaagugcc cuccaagaaa      300

augaaggucc uuggaaauac cucuaaaaag uacauuaaga aaaaccugcu cggcgugcug      360

cuuuucgaua gcgggaucac agcugaagga cggcgccuga agagaaccgc ccggcgcaga      420

uauacacggc gcagaaaucg gauucucuac cugcaggaga ucuuuuccac cgaaauggcc      480

acacuggacg augcuuucuu ucaacgccuc gacgauucuu uccugguucc ugacgauaaa      540

agagacagca aguaucccau uuuuggcaac cuuguggagg aaaaagucua ccaugaugag      600

uucccuacca ucuaucaucu gcggaaguac cucgccgacu ccacgaaaaa ggcugaucug      660

cgccuugugu accuggcccu cgcgcacaug auuaaguaua gaggccacuu ucugaucgag      720

ggggaguuca auagcaaaaa caaugacauc cagaagaacu uucaggacuu ccuggauacc      780

uacaaugcca uuuuugaauc ugaccucucc cuggaaaaca gcaaacagcu ugaggagauc      840

guuaaggaua aaauuucuaa gcuggaaaag aaagaccgga uccucaagcu guuccccgga      900

gagaaaaauu ccggcauuuu cagcgaauuu cuuaagcuga ucguggggaa ccaagcugau      960

uuccgcaaau gcuuuaaccu cgacgagaag gccucucugc auuucuccaa ggaaagcuau     1020

gaugaggacc uggagacacu ccugggauac aucggcgaug acuauuccga ugucuuucuu     1080

aaagcuaaga aacuguacga cgccauucuc cugucugggu uccuuaccgu gacagacaau     1140

gaaaccgagg cgccucugag cuccgccaug aucaagagau auaacgaaca caaagaggau     1200

cucgcucugc ugaaggaaua cauucggaau aucagccuca agacguacaa cgagguguuu     1260

aaagacgaua caaagaaugg auaugccggc uacauugacg gcaaaaccaa ccaggaggau     1320

uucuaugucu accugaagaa ucuucuggcu gaauuugagg gggccgacua uuuccucgaa     1380

aagaucgaua gagaggacuu ucugcgcaag cagcggacau ucgauaacgg aucuaucccg     1440

uaccagauuc aucugcaaga aaugagagcc auccuugaca aacaggcuaa guuuuauccc     1500

uuccucgcga agaauaaaga gcgcauugag aagauccuga ccuuccggau uccuuacuac     1560

guuggccccc uggccagagg gaacuccgau uuugcuugga gcauccgcaa acggaaugaa     1620

aagauuaccc cuuggaacuu cgaggacgug aucgauaaag aaucuagcgc cgaggccuuu     1680

aucaauagaa ugacauccuu cgaccucuau cugcccgagg aaaaggugcu uccgaagcac     1740

ucucugcucu acgagacguu uaacgucuau aaugaacuga ccaaagugcg cuucauugcu     1800

gagagcaugc gggacuacca guuucuggau uccaagcaga aaaaggacau cguuagacuu     1860

uauuucaaag auaagcgcaa ggugacagac aaagauauua ucgaauaccu ccacgccauu     1920

uauggauacg acggcaucga gcugaagggg aucgagaaac aauuuaacag cucucugucc     1980

accuaccaug aucuccugaa cauuaucaau gacaaggagu uccuugauga cagcucuaac     2040

gaagcgauua ucgaagagau uauccacaca cugaccaucu uugaagaucg ggagaugauu     2100

aaacagagac ucuccaaguu cgagaauauc uuugacaaga gcguccugaa aaagcugucc     2160

cggcgccacu auacgggaug gggcaaacuu ucugcuaagc ucauuaacgg caucagagau     2220

gaaaagagcg ggaauacaau ucuggacuac cugaucgacg auggaaucuc caaccggaau     2280

uucaugcagc ucauucauga cgaugcccug ucuuucaaga aaaagaucca aaaggcccag     2340

auuaucggcg acgaggauaa agggaacauu aaggaagugg uuaaaagccu uccuggaucc     2400

cccgcuauca agaaaggcau ucugcagagc aucaagaucg uggacgagcu cgucaaggug     2460

augggcggga gaaaaccuga aucuauuguc guggagaugg cccgcgagaa ucaguauacc     2520

aaccaaggaa aguccaauag ccagcagcgg cugaaaagac uggaaaaguc ucuuaaagag     2580

cucggcucca agauccugaa ggaaaacauu cccgcuaaac ugagcaagau cgauaauaac     2640

gcccuccaga augaccgccu guaccuuuau uaccugcaaa acgggaaaga uauguauacc     2700

ggagacgauc ucgacauuga cagacugucu aacuacgaua ucgaccacau cauuccccag     2760

gcguuucuga aggauaauag caucgacaac aaaguucuug uguccagcgc cucuaaucgg     2820

ggcaaguccg augacuuccc uagccuggag gucgugaaga aacgcaagac auuuugguac     2880

cagcuccuga aauccaagcu cauuucucag agaaaauucg auaaccugac caaggcugag     2940

cgcgggggac uucugccgga agacaaggcc ggcuuuaucc aacggcagcu cguugagaca     3000

agacagauua cgaaacaugu ggcucggcug cuggaugaaa aguucaauaa caaaaaggac     3060

gagaauaacc gcgccgucag aaccgugaaa aucaucacac uuaagagcac ccuggugucc     3120

caguuucgga aagauuucga acucuauaag guucgcgaga uuaaugacuu ucaccaugcg     3180

cacgaugccu accugaacgc ugucaucgcc ucugcucucc ugaagaaaua ucccaaacuu     3240

gagccugagu ucguguacgg ggacuauccc aaauacaaua gcuuuagaga acggaagucc     3300

gccacagaaa aaguguauuu cuacucuaac auuaugaaua ucuucaaaaa gagcauuucc     3360

cuggccgacg gcagagucau cgagcgcccu cucauugaag ugaacgagga gaccggagaa     3420

agcguuugga auaaagaguc ugaucuggcu acggugcgga gaguccuguc cuacccccaa     3480

gugaacguug ugaagaaagu cgaggaacag aaccacggcc uugaccgcgg gaagccgaaa     3540

ggacuguuua augcgaaccu cagcucuaag ccuaagccca auuccaacga aaaucuggug     3600

ggcgccaaag aguaucucga uccuaagaaa uacgggggau augcuggcau cagcaacucu     3660

uucgccguuc uggugaaggg gaccaucgag aaaggcgcca agaagaaaau uacaaauguc     3720

cuugaauuuc agggaaucuc cauucuggac cggaucaacu acagaaagga uaaacugaau     3780

uuccuccugg agaagggcua uaaagacauu gaacuuauca ucgagcugcc caaguacagc     3840

cucuuugagc uguccgaugg gagccgccgg augcucgcuu cuauucugag caccaacaau     3900

aaaagaggag aaauccauaa gggcaaccaa auuuuccuuu cccagaaguu ugugaaacug     3960

cuguaucacg ccaagcgcau cucuaauaca auuaacgaga aucaucggaa auacguggaa     4020

aaccacaaga aagaguucga ggaacucuuu uacuauaucc uggaguucaa ugaaaacuac     4080

gucggggcga agaagaacgg aaaacuucug aauagcgcuu uucaguccug gcagaaccac     4140

ucuaucgacg agcucuguag cuccuucauu ggcccuaccg ggucugaaag aaagggccug     4200

uuugagcuca cgagccgggg auccgccgcc gauuucgagu uccugggcgu uaaaaucccc     4260

cgcuauagag acuacacacc gagcucucuu cugaaggaug cuacccugau ucaucaaucc     4320

gugacagggc ucuaugaaac ccggaucgac cuggccaaac uuggagaggg caagcgcccu     4380

gcggcuacca agaaagccgg gcaggccaag aaaaagaaau aaggacuagu guccaccugu     4440

cccuccuggg cugcuggauu gucucguuuu ccugccaaau aaacaggauc agcgcuuuac     4500

agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4560

aaaaaaaaaa gaauu                                                      4575


<210>  4830
<211>  3651
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4830
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccugg uacgcgagcc ugauguccgc ucaucaccuc      180

cggguuggaa uugacguggg cacccauucu guggggcugg ccacacuucg cgucgaugac      240

cacggaacgc cgaucgagcu gcucagcgcc cugucccaua uucacgauuc uggcgugggg      300

aaggaaggga aaaaggacca cgauaccaga aagaaacuua gcggcaucgc ucggcgcgcc      360

agacggcugc uccaucaccg cagaacacag cugcaacagc uggacgaggu ucuccgggau      420

cugggcuucc ccauuccuac ccccggggaa uuucuugacc ugaacgagca gacagauccu      480

uaucgcgugu ggagaguccg ggcucgccuc guggaagaga agcugcccga ggaacuuaga      540

ggaccugcca ucuccauggc gguucggcau aucgcccgcc acagaggcug gcggaauccg      600

uacucuaaag uggagagccu gcucuccccc gcugaagaga gcccuuucau gaaggcccug      660

agagagcgca uucuggcuac cacaggggaa guccucgacg auggaaucac ccccggccag      720

gccauggcgc aaguggcccu gacgcacaac auuucuaugc gggggccuga gggaauccuu      780

ggcaaacugc aucaguccga caaugcuaac gaaauuagaa agaucugcgc ccgccagggc      840

gugagccccg augucuguaa gcagcuccug cgggcuguuu uuaaagccga cucuccgaga      900

ggguccgccg ugagccgcgu ggcuccugac ccccuuccug gacaaggcuc uuuccggaga      960

gcgcccaagu gcgaucccga guuucagcgc uuccggauua ucuccaucgu cgccaaucug     1020

agaauuagcg aaaccaaagg ggagaaccgc ccucucacag cugacgagcg gagacacgug     1080

guuaccuuuc ugacagaaga uucccaggcc gaccugaccu ggguggaugu cgccgagaag     1140

cucggagugc aucggcgcga ccugagaggc acggcuguuc acacagauga cggggaacgg     1200

ucugccgcga gaccgcccau cgaugcuacc gaccgcauua ugcggcagac aaaaaucagc     1260

ucccuuaaga ccugguggga ggaagccgau agcgagcaaa gaggagccau gauucgcuau     1320

cuguacgagg acccuaccga cucugaaugu gcugagauca ucgccgaacu ccccgaggaa     1380

gaucaggcua aacuggacuc ccuucaucug ccugccggca gagcggccua uagccgggag     1440

ucucucacag cucugagcga ucacaugcug gccacgaccg acgaucucca cgaggcucgc     1500

aagagacugu ucggcgugga cgauuccugg gcgcccccgg ccgaagccau uaaugcuccu     1560

gucgggaacc ccucugugga ccggacacuu aagaucgucg gacgcuaccu gagcgccgug     1620

gaguccaugu ggggcacccc ugaaguuauu cauguggagc acgucagaga uggguuuaca     1680

agcgagcgga uggcugacga acgcgauaaa gccaauagac ggcgcuauaa cgacaaucag     1740

gaggccauga agaaaaucca aagagauuac ggaaaggaag gcuacauuuc ucggggagac     1800

aucgugagac ucgacgcucu ggagcugcag ggcugcgcgu gucuuuauug cggaaccacg     1860

aucggcuacc auacauguca gcucgaucac auuguucccc aggccgggcc uggauccaac     1920

aaucgccggg gcaaccuggu ggcugucugc gaaagaugua aucgcagcaa aucuaacacc     1980

cccuucgccg ugugggccca aaagugcggg aucccgcacg ugggaguuaa ggaggcuauu     2040

ggccggguca gaggguggcg caaacagacc ccuaacacau ccagcgagga ccugacccgg     2100

cucaagaaag aagugaucgc cagacugcgc cggacacagg aggaucccga aauugacgag     2160

agauccaugg aaucuguggc guggauggcu aaugagcuuc aucaccggau cgccgccgcu     2220

uaucccgaga cgaccgucau gguguaccgc ggcagcauua cagccgcggc uagaaaggcc     2280

gccggaaucg auucccggau caaccugauu ggcgaaaaag ggcgcaagga cagaaucgau     2340

cggagacauc acgcuguuga cgccucugug gucgcucuca uggaggccag cguggcgaag     2400

acccuggccg aacgcuccag ccugcgggga gagcagagac uuacaggcaa agaacaaacc     2460

uggaagcagu auacgggguc uaccguugga gcucgcgagc auuuugagau guggcggggc     2520

cacaugcucc accugacaga acuguucaau gagagacucg ccgaagauaa aguguacguc     2580

acccagaaca uucgccugcg gcuuuccgac gggaaugcuc auacagugaa cccuagcaag     2640

cugguuucuc acagacucgg cgauggacug accgugcagc aaaucgaccg cgccuguacg     2700

ccggcccugu ggugcgcucu uacaagagag aaagauuuug acgagaagaa uggccucccc     2760

gcgcgggaag accgcgccau uagaguccau gggcacgaga ucaaguccag cgauuauauc     2820

cagguguucu cuaaacggaa gaaaaccgac agcgaucgcg acgaaacacc uuucggagcu     2880

auugccguga gaggcggguu ugucgagauc ggacccucca uucaucacgc ccggaucuac     2940

agaguugaag gcaagaaacc uguguacgcu augcugcgcg uguucaccca cgaucugcuc     3000

agccagcggc auggggaccu guuuucugcc gucauucccc cucaguccau cagcaugaga     3060

ugcgcggagc ccaaacuucg caaagcuauc accacaggca acgccacgua ucugggaugg     3120

gugguugugg gcgaugagcu cgaaauuaau gucgacuccu ucaccaagua cgccaucggg     3180

cgguuucugg aggauuuccc gaacacaacc agauggcgga uuuguggaua ugacacaaau     3240

ucuaagcuga cccuuaaacc uaucgugcuc gcugccgaag gccuggagaa ccccagcucc     3300

gcgguuaaug aaauugugga gcugaagggg uggcgcgucg cuaucaacgu gcucacgaaa     3360

guucacccua caguggucag acgggaugcc cugggacgcc ccagauacuc uagccggucc     3420

aaucuuccga ccucuuggac caucgagaag cgcccugccg cuacaaagaa ggccggccaa     3480

gcuaagaaaa agaaauaagg acuagugucc accugucccu ccugggcugc uggauugucu     3540

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              3651


<210>  4831
<211>  4512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4831
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccacc aagccguaca gcauuggacu ggacaucggc      180

acaaacuccg uugggugggc ggugacgacc gauaauuaua aagugcccuc uaagaagaug      240

aaaguccucg gaaacacaag caagaaauac auuaagaaaa aucugcuugg cgugcuccug      300

uucgacuccg ggaucaccgc ugagggacgg cgccugaaga gaacagcccg gcgcagauau      360

acccggcgca gaaaccggau ucuuuaccug caggaaaucu uuucuacaga gauggccacc      420

cucgaugacg cuuucuuuca acgccuggau gacagcuucc ugguuccuga ugacaagaga      480

gauuccaaau aucccaucuu uggcaaucuc guggaagaga aggccuacca ugacgaguuc      540

ccuacgauuu aucaucugcg gaaauaccuu gcugauucua ccaagaaagc cgaccugcgc      600

cucgucuacc uggcgcuugc ccacaugauc aaguauagag gccacuuucu gauugagggg      660

gaguucaaca gcaaaaauaa cgauauccag aagaauuuuc aggacuuccu cgacacauac      720

aacgcuauuu uugaauccga ucugagccug gaaaauucua agcagcucga agagaucgug      780

aaagacaaga uuuccaaacu ggagaagaaa gaucggaucc uuaagcuguu ccccggagaa      840

aagaacagcg gcaucuuuuc ugaguuccuc aaacugauug uugggaauca agccgacuuc      900

cgcaagugcu uuaaccuuga ugaaaaggcu ucccugcauu ucagcaaaga gucuuaugac      960

gaagaucucg agacccugcu gggauacauc ggcgacgauu auuccgacgu guuucucaaa     1020

gccaagaagc uguacgaugc gauucuucug agcggguucc ucacagucac cgacaaugag     1080

acggaagccc cucuguccuc ugcuaugauc aaaagauaua acgagcacaa ggaagaccuu     1140

gcccugcuca aagaguacau ucggaacauc agccugaaga cauacaauga aguguuuaaa     1200

gaugacacca agaacggaua ugcuggcuac aucgauggca agacaaauca ggaggacuuc     1260

uauguguacc ugaaaaagcu ccuggccgag uuugaagggg ccgauuauuu ccuugagaaa     1320

auugacagag aagauuuucu gcgcaagcag cggaccuucg acaacggauc caucccguac     1380

cagauucacc uccaagagau gagagcuauc cuggauaaac aggcgaaguu uuaucccuuc     1440

cuggccaaaa auaaggaacg cauugagaag auccuuaccu uucggauccc uuacuacguc     1500

ggcccccucg cuagagggaa cagcgacuuc gccuggucua uucgcaaacg gaaugagaag     1560

aucacaccuu ggaacuucga agauguuauu gacaaagagu ccagcgccga ggcuuuuauc     1620

aauagaauga cgucuuucga ucuguaucug cccgaagaga aggugcuccc gaaacauagc     1680

cugcuuuacg aaaccuuuaa cguguauaau gagcugacaa agguccgcuu cauugccgaa     1740

uccaugcggg acuaccaguu ucucgacucu aagcagaaaa aggauaucgu gagacuguau     1800

uucaaagaca agcgcaaagu uaccgauaag gacauuaucg aguaccugca cgcgaucuau     1860

ggauacgaug gcauugaacu uaaggggauc gagaaacaau uuaacagcuc ccucagcaca     1920

uaccaugacc ugcugaauau uaucaacgau aaggaguucc ucgacgauuc uuccaaugaa     1980

gcuauuaucg aggaaaucau ucacacccug acgaucuuug aggaccggga aaugauuaaa     2040

cagagacuua gcaaguucga gaacaucuuu gauaaaucug ugcugaagaa gcucucccgg     2100

cgccacuaua caggaugggg caaacugagc gccaagcuga uuaacggcau cagagacgag     2160

aaauccggga auaccauccu ugauuaccuc auugacgacg gaaucucuaa ccggaauuuc     2220

augcagcuga uucaugauga cgcccugagc uuuaagaaaa agauccaaaa agcucagauu     2280

aucggcgaug aagacaaggg gaacaucaag gaggucguga aaucccuccc uggaucuccc     2340

gccauuaaga aaggcauccu gcagagcauu aagaucguug augaacuugu gaaagucaug     2400

ggcgggagaa agccugaguc cauugugguc gaaauggcuc gcgagaauca guauaccaac     2460

caaggaaaga gcaauucuca gcagcggcug aaaagacucg agaagucccu gaaagaacug     2520

ggcagcaaga uccuuaaaga gaacauuccc gccaagcucu cuaagaucga caauaacgcg     2580

cugcagaaug aucgccugua ccucuauuac cugcaaaacg ggaaagacau guauacagga     2640

gaugaccuug auaucgacag acuguccaau uacgacauug aucacaucau uccccaggcc     2700

uuccucaagg acaacagcau cgauaauaaa gugcugguuu cuagcgcuuc caaccggggc     2760

aagagcgacg augugccuuc ucuggaaguc gugaaaaagc gcaagaccuu cugguaccag     2820

cuucugaaau ccaagcucau uagccagaga aaauuugaca accugacaaa ggccgagcgc     2880

gggggacucu ccccggagga uaaagcuggc uucauccaac ggcagcuggu ugaaacgaga     2940

cagaucacca agcauguggc ccggcuucug gacgagaagu uuaauaacaa aaaggaugaa     3000

aauaaccgcg cggucagaac agugaaaauu aucacccuca agucuacacu ggugagccag     3060

uuccggaaag acuuugagcu guauaagguu cgcgaaauua augauuucca ccaugcccac     3120

gacgcuuacc uuaacgccgu cguggcuucc gcccugcuca aaaaguaucc caagcuggag     3180

ccugaguuug uguacgggga uuaucccaaa uacaauucuu ucagagaaag aaagagcgcc     3240

accgagaaag ucuauuuuua cuccaacauc augaauauuu ucaagaaauc uaucagccuc     3300

gcugacggcc gggugaucga acgcccucug auugagguua acgaagagac gggagagucc     3360

guguggaaua aggaaagcga ccuugcgacc guccggagag ugcugucuua cccccaaguu     3420

aacgugguca agaaagugga ggaacagaau cacggccucg aucgcgggaa gccgaaagga     3480

cuguuuaacg ccaaucuguc cagcaagccu aaacccaacu cuaacgagaa ucuuguuggc     3540

gcuaaggaau aucuggaccc uaagaaauac gggggauaug ccggcaucuc caacagcuuc     3600

acagugcucg ucaaggggac cauugagaaa ggcgccaaga aaaagaucac aaaugugcug     3660

gaguuccagg gaauuucuau ccucgaucgg auuaacuaca gaaaggacaa acugaauuuu     3720

cuucuggaaa agggcuauaa agauaucgag cugaucauug aacuccccaa guacucccug     3780

uucgagcuua gcgacggguc ccgccggaug cuggcuagca uccucucuac caacaauaaa     3840

agaggagaga uucauaaggg caaccaaauc uuucugagcc agaaauucgu gaagcuccug     3900

uaucacgcca agcgcauuuc caauacgauc aacgaaaauc aucggaaaua cgucgagaac     3960

cacaagaaag aauuugagga acuuuucuac uauauccugg aguuuaauga gaacuacguu     4020

ggggcgaaga aaaauggaaa gcugcucaac ucugcuuucc agagcuggca gaaucacucc     4080

auugaugaac uguguucuag cuuuaucggc ccuacagggu ccgagagaaa gggccuuuuc     4140

gaacugaccu cucggggaag cgccgccgac uuugaguucc ucggcgugaa aauuccccgc     4200

uauagagauu acacaccguc cagccugcuc aaggacgcua cccugaucca ucaaucugug     4260

accgggcuuu augaaacacg gauugaucug gccaaacugg gagagggcaa gcgcccugcg     4320

gcuacgaaaa aggccgggca ggccaagaaa aagaaauaag gacuaguguc caccuguccc     4380

uccugggcug cuggauuguc ucguuuuccu gccaaauaaa caggaucagc gcuuuacaga     4440

ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4500

aaaaaaagaa uu                                                         4512


<210>  4832
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4832
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccgcg gcuuucaagc cgaacagcau uaauuacauc      180

cugggacucg acauuggcau cgccuccguu gggugggcca ugguggagau ugaugaagag      240

gaaaacccca uccggcugau cgaccuugga gugcgcgucu uugagagagc ugaagugccu      300

aaaaccggcg auucucuggc cauggcucgg cgccucgcca gaagcguucg gcgccugaca      360

agacggcgcg cgcauagacu ucugcggacg cgcagacucc ugaagcggga gggggugcug      420

caggccgcua auuucgacga gaacggacuc auuaaguccc ugcccaauac cccuuggcaa      480

cuucgcgccg cugcccugga uagaaaacuc acaccccugg aauggucugc gguccuucug      540

caccucauca agcaucgggg cuaucugagc cagcgcaaaa acgagggcga aaccgccgac      600

aaggagcugg gggcucuccu gaaaggagug gccggcaaug cucacgcccu ucagacaggg      660

gauuuuagaa ccccugccga gcuggcucuc aacaaguucg aaaaggaguc cggacauauu      720

cggaaucaga gaucugacua cagccacaca uuuucccgca aagaucugca agcggaacuu      780

auccuccugu ucgagaagca gaaagaauuu ggcaacccgc acguuagcgg gggacugaag      840

gagggcauug agacccugcu caugacgcag cggcccgccc ugucuggcga cgcugugcag      900

aaaaugcuug ggcauugcac cuucgaaccu gccgagccca aggccgcuaa aaauacauau      960

accgccgaaa gauuuaucug gcugacaaag cucaacaauc ugcgcauucu ugagcaagga     1020

uccgaacggc cucugaccga uacggagaga gcgacacuca uggacgagcc cuaccgcaag     1080

agcaaacuga ccuaugcuca ggcccggaag cugcucggcc uggaagauac agccuucuuu     1140

aaagggcuua gauacggaaa ggacaacgcu gaggccucua cccugaugga aaugaaggcu     1200

uaucacgcca ucucccgcgc gcucgagaag gaaggccuga aggacaaaaa gagcccgcug     1260

aaucuuucuc cugagcucca ggaugagauc gggaccgccu ucucccuguu uaaaacagac     1320

gaagauauua cgggacggcu gaaggacaga auccagcccg agauucucga agcucugcuu     1380

aaacauauca gcuucgauaa guucguccaa auuucccuga aggcccuccg gcgcaucgug     1440

ccucugaugg agcagggcaa aagauacgac gaggcuugug ccgaaaucua cggcgaucac     1500

uaugggaaga aaaacaccga ggaaaagauu uaccugccuc ccauccccgc ggacgagauu     1560

cggaacccgg ugguccuuag agcccucucu caggcucgca aaguuaucaa uggaguggug     1620

cggagauaug gcagccccgc ccgcauucac aucgaaacag cuagagaggu cgggaagucc     1680

uuuaaggauc ggaaagagau cgaaaagcgc caagaggaaa acagaaaaga ccgggagaag     1740

gccgccgcua aauuccgcga auacuuuccu aauuucgugg gagagcccaa gagcaaagau     1800

auucugaagc ugagacucua ugagcagcag cauggcaagu gccuguacuc ugggaaagaa     1860

aucaaccuug gccggcugaa ugagaaggga uauguugaaa uugaccacgc gcucccuuuu     1920

ucccgcaccu gggacgauag cuucaacaau aaagugcugg uccugggcuc ugagaaccag     1980

aauaagggga accaaacacc cuacgaauac uuuaauggaa aagacaacag cagagagugg     2040

caggaguuca aggcccgggu ggaaaccucc cgcuuuccga gaucuaagaa acagcggauc     2100

cuucuccaga aguucgauga ggacggcuuu aaagaaagaa aucugaacga uacgcgcuau     2160

guuaaucggu uccuguguca auuuguggcu gacagaaugc gccucacagg gaagggcaaa     2220

aagcgggucu ucgccagcaa cggacagauu accaaccugc uuagaggguu cuggggccug     2280

cgcaaggugc gggccgagaa ugauagacau cacgcucucg acgccgucgu gguugcgugc     2340

uccaccgugg cuaugcagca gaaaaucaca cgcuuugucc gguacaagga gaugaacgcc     2400

uucgauggaa aaaccauuga caaggaaaca ggcgaggugc ugcaucagaa aacgcacuuu     2460

ccucagcccu gggaguucuu ugcccaggaa guuaugauca gaguguucgg gaagccugau     2520

ggaaagcccg aauuugagga ggcugacacc cuggaaaaac uucggacacu ccuggccgag     2580

aagcugagcu cucgcccuga agcgguccac gaguauguga caccccucuu cguguccaga     2640

gcuccgaauc ggaaaaugag cggccagggg cauauggaaa ccguuaaguc ugccaaacgc     2700

cuggaugagg gagucuccgu gcuuagagug ccucugaccc aacucaagcu gaaagaccug     2760

gagaagaugg ucaaccggga aagagagccc aaacuuuacg aagcccugaa agcucgccuc     2820

gaggcccaca aggacgaucc cgcuaaagcc uuugcggaac cuuucuauaa guacgacaaa     2880

gccggcaauc ggacgcaaca ggugaaggcu guuagagugg agcaagucca gaagaccggg     2940

guguggguuc gcaaccauaa uggcaucgcc gauaacgcua caauggugcg ggucgacgug     3000

uuugagaaag gagauaagua uuaccugguu ccgauuuaca gcuggcaagu ggccaaaggc     3060

auccuccccg acagagccgu cgugcagggg aaggaugaag aggacuggca gcugauugau     3120

gacuccuuca auuuuaaauu cucucuucac ccuaacgauc ugguggaagu caucaccaag     3180

aaggcucgca uguucggaua uuuugcgagc ugucaccggg gcacagggaa uauuaacauc     3240

agaauccaug accucgauca caaaauugga aagaauggca uccuggaggg gauuggcguu     3300

aaaaccgccc uguccuucca gaaguaccag aucgacgagc uuggaaaaga aauucgcccc     3360

ugcagacuga agaagcggcc ucccgugcgc aaaagaccug cugccacgaa gaaagccggc     3420

caggcuaaga aaaagaagua aggacuagug uccaccuguc ccuccugggc ugcuggauug     3480

ucucguuuuc cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           3594


<210>  4833
<211>  4350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4833
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaag aaaccguaca ccauuggacu ggacaucggc      180

acaaacagcg uugggugggc ggugcucacg gaucaguaug accuggugaa gcggaagaug      240

aaaauugcug gagauuccga gaagaaacaa aucaagaaaa auuucugggg cguccgccuu      300

uuugacgaag ggcagaccgc cgccgauaga cggauggcuc gcacagccag acggcgcauu      360

gagagacggc gcaacagaau cucuuaccug cagggaaucu ucgcugaaga gaugagcaag      420

accgacgcca auuucuucug ccggcucucc gauucuuuuu auguggacaa cgaaaagcgc      480

aauagcagac aucccuucuu ugcgacaauu gaggaggaag uugaguacca caaaaacuau      540

ccuaccaucu accaucugcg ggaggaacuu gucaauuccu cugagaaggc cgaucugcgc      600

cucgucuauc uggcucuggc ccacauuauc aaauacagag gcaacuuccu cauugaaggc      660

gcucuggaca cacagaauac cagcguggau gggaucuaca agcaauuuau ucagacguau      720

aaccagguuu ucgccuccgg aaucgaggac ggcagccuua aaaagcugga agauaauaaa      780

gacguggcga agauccucgu cgagaaggug acccggaaag aaaagcugga gcgcauucuu      840

aaacuguacc ccggggagaa gucugccgga auguuugcuc aguucaucuc ccucauugug      900

ggcagcaaag ggaacuuuca aaagccuuuc gaccugaucg aaaagucuga uauugagugu      960

gccaaagacu ccuaugaaga ggaucuggaa agccuccugg cucuuaucgg agacgaguac     1020

gccgagcugu ucgucgccgc uaagaaugcg uauucugccg uugugcucuc cagcaucauu     1080

acaguggcug aaaccgagac aaacgccaaa cuguccgccu cuaugaucga aagauuugau     1140

acccaugagg aagaccuugg cgagcugaag gcuuucauua aacuccaucu gcccaagcac     1200

uacgaggaaa ucuuuagcaa uacggagaag cacggcuaug ccggguacau ugauggaaaa     1260

acaaagcagg cggacuucua caaauauaug aagaugaccc uggaaaacau cgagggcgcu     1320

gauuacuuua ucgccaaaau ugagaaggaa aacuuccucc ggaagcagag aacauuugac     1380

aauggggcca ucccucacca gcugcaucuu gaggaacugg aggcuauucu ccaccaacag     1440

gccaaauauu acccguuccu gaaggaaaac uaugauaaaa ucaagucccu ugucaccuuu     1500

cgcauucccu acuucguggg accucuggcu aauggccaga gcgaguuugc cuggcucacc     1560

cggaaagcgg acggggagau cagacccugg aacauugaag agaagguuga cuucggaaaa     1620

ucugccgugg auuuuaucga aaagaugaca aauaaggaca cguaucugcc uaaagagaac     1680

guccugccca agcacucccu cugcuaccaa aaauaccugg uguauaauga acuuaccaag     1740

guucgcuaca ucaacgauca gggcaaaaca agcuauuucu cuggccagga gaaggagcag     1800

auuuucaaug accuguuuaa gcaaaaacgg aaggugaaaa agaaagaucu cgaacuguuc     1860

cugagaaaca ugagccaugu cgaguccccg accaucgaag ggcuugagga cucuuuuaau     1920

agcuccuaca gcacauauca cgaucuccug aaggugggaa uuaagcagga aauccuggac     1980

aacccuguca auaccgagau gcucgagaac auugugaaaa uccugacggu uuucgaagau     2040

aagcgcauga ucaaagagca gcuucagcaa uuuucugacg ugcuggaugg cgucgugcuc     2100

aagaaacugg aacggagaca uuacacaggg uggggacgcc uguccgcuaa gcuccucaug     2160

ggcauucggg acaagcagag ccaccugacc auccuggauu aucucaugaa ugacgacggg     2220

cugaacagaa accuuaugca gcugauuaau gauucuaacc ucuccuucaa aagcaucauu     2280

gagaaggagc agguuaccac agccgacaaa gauauccaau ccaucguggc ugaccuggcc     2340

ggcucucccg cgauuaagaa aggaauccug cagagccuua agauugucga ugaacucgug     2400

uccgugaugg gcuacccucc ccagaccauc guugucgaga uggcccggga aaaucagaca     2460

acggggaaag gaaagaacaa uucucgcccc agauacaaga gccuggagaa agcuauuaag     2520

gaauuuggcu cccaaauccu gaaagagcau ccuaccgaca accaggagcu ccggaauaac     2580

agacuguauc uuuacuaucu gcagaauggg aaggauaugu acacaggaca agaccucgau     2640

auccacaacc ugagcaauua ugacauugau cacaucgugc cgcagucuuu cauuaccgac     2700

aacuccaucg auaaucuggu gcuuacaagc ucugccggca accgcgaaaa aggggacgac     2760

gugcccccuc ucgagauugu gcggaagaga aagguguuuu gggaaaaacu guaccagggc     2820

aaucugaugu ccaagcgcaa auucgauuau cucaccaagg cugagagagg aggccugacg     2880

gaagccgaca aagcccgguu uauccaucgc cagcuuguug agaccagaca aauuacaaag     2940

aacgucgcua acauccugca ccagcgguuc aauuacgaga aggaugacca ugggaacacc     3000

augaaacagg ugcgcaucgu uacacucaag agcgcgcugg ugucucaguu uagaaaacaa     3060

uuccagcugu acaagguccg ggaugugaau gacuaucacc acgcccauga ugcuuaccuu     3120

aacggaguug uggccaauac ccugcucaaa gucuaucccc agcuggaacc ugaguucgug     3180

uacggcgacu aucaccaguu ugauugguuc aaggccaaca aggcuacggc caaaaagcaa     3240

uuuuacacaa auauuaugcu uuucuuugcg cagaaagacc gcaucauuga ugaaaacggg     3300

gagauccugu gggacaagaa auaucuugac accgugaaga aggucaugag cuacagacag     3360

augaauauug uuaaaaagac agaaauccaa aaaggagagu ucuccaaggc uaccaucaaa     3420

cccaagggca acagcucuaa acugauuccg cggaagacca auugggaucc uaugaaguac     3480

gggggccucg acucccccaa cauggccuau gccgugguga ucgaguacgc uaaaggaaag     3540

aauaaacugg ucuuugaaaa gaaaauuauc cgcgugacaa uuauggagag aaaggccuuc     3600

gaaaaggaug agaaagcguu ucuggaggaa cagggcuauc ggcagccuaa gguucuugcu     3660

aaacugccca aguacacgcu cuaugagugu gaagagggga gacgccggau gcuggccagc     3720

gccaacgaag cucagaaagg aaaucaacag gugcucccua accaucuggu cacccuucug     3780

caccaugccg cuaacugcga gguguccgac ggcaagucuc ucgauuacau cgagagcaau     3840

agagaaaugu ucgccgagcu gcuggcgcac guuuccgaau uugccaagcg cuauacacuu     3900

gcugaggcca accugaauaa aaucaaccag cucuuugaac agaauaagga gggggacauu     3960

aaagcuaucg cccaaucuuu cguggaucug auggccuuua acgcuauggg agcgcccgcc     4020

agcuucaagu uuuucgagac cacaauugaa cggaaaagau acaauaaccu caaggagcug     4080

cuuaauucca ccaucauuua ccagucuauc acgggccugu augaaagccg caagcggcug     4140

gacgauaaaa gaccggcugc cacaaagaaa gccgggcagg cuaagaaaaa gaaauaagga     4200

cuagugucca ccugucccuc cugggcugcu ggauugucuc guuuuccugc caaauaaaca     4260

ggaucagcgc uuuacagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaagaauu                                      4350


<210>  4834
<211>  4383
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4834
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaag aaaccguaca gcauuggacu ggacaucggc      180

accaacuccg uugggugggc gguggugaca gaugacuaua agguccccgc uaagaaaaug      240

aaggugcucg gaaauacgga uaaaucucau auugagaaga accugcuugg cgcccuccug      300

uucgacagcg ggaauaccgc cgaagaucgg cgccugaaaa gaacagcucg gcgcagauac      360

acccggcgca gaaaccggau ccuuuaucug caggagauuu ucuccgaaga gaugggaaag      420

guugacgauu cuuucuuuca ccgccucgaa gacagcuucc uggugacaga ggauaagaga      480

ggcgagcggc auccuaucuu uggcaaucug gaagaggaag ucaaauacca cgagaacuuc      540

cccaccaucu aucaucuccg ccaauaccug gccgacaauc cugagaaggu ggaucuuaga      600

cugguuuauc ucgcucuggc ccacauuauc aaauuucggg ggcacuuccu uauugaagga      660

aaguuugaca cacgcaacaa ugaugugcag agacuguucc aggaguuucu cgcggucuac      720

gacaacaccu ucgaaaauuc cucucugcag gagcaaaacg uccaggugga agagauccug      780

acggacaaaa uuagcaaguc cgccaaaaag gaucgggugc ucaagcuguu ucccaaugag      840

aaaagcaacg gcagauucgc ugaauuucuu aagcugaucg uugggaauca ggccgacuuu      900

aaaaagcauu ucgagcucga agagaaagcu ccucugcagu uuucuaagga uaccuacgaa      960

gaggagcuug aagugcugcu cgcccaaauu ggagacaacu augcggagcu guuccugucc     1020

gccaagaaac ucuacgauag cauccugcuu ucuggcaucc ugacagugac cgacgucggg     1080

acaaaggcuc cgcucuccgc cagcaugauu cagcgcuaua acgaacacca gauggaucug     1140

gcucagcuua aacaauuuau ccggcagaag cugucugaca aauacaauga gguguucucc     1200

gauguuagca aggacggaua ugccggcuac auugauggca agaccaacca ggaagccuuu     1260

uauaaauacc ucaaggggcu gcugaauaaa aucgagggau ccggcuacuu ccucgacaag     1320

auugagagag aagacuuucu gcgcaaacaa cggacguucg auaacggguc uaucccccau     1380

cagauccacc uucaggagau gagagcuauu auccgccggc aggcggaauu uuauccuuuc     1440

cuggccgaca aucaagauag aauugagaag cuccugacau uucgcauccc cuacuaugug     1500

ggaccucugg cucggggcaa gagcgacuuc gccuggcuuu ccagaaaaag cgccgauaag     1560

auuacccccu ggaacuucga cgagaucguc gauaaagaau cuuccgcuga ggccuuuauc     1620

aaucgcauga caaacuacga ccucuaucug ccgaaucaga aggugcugcc uaaacacagc     1680

cuccuguacg aaaaguucac cguuuauaac gagcuuacca aagugaagua caagacagaa     1740

caggggaaaa cggcguuuuu cgaugcuaau augaagcagg agauuuuuga cggagucuuc     1800

aaaguguacc ggaaggucac caaagauaag cugauggacu uucucgagaa ggaguucgau     1860

gaauuuagaa ucguggaccu gacaggccug gacaaagaaa acaagguuuu caaugccucu     1920

uauggcaccu accaugaucu uugcaaaauu cucgacaagg auuuucugga caacagcaaa     1980

aaugagaaga uccuggaaga uauugugcuc acacugaccc uuuucgagga ccgggagaug     2040

auccgcaaga gacuggaaaa cuauuccgau cuccugacga aagagcaagu caagaaacug     2100

gaacggagac acuacacagg guggggacgc cuuucugccg agcucauuca uggcauccgg     2160

aacaaggaaa gcagaaaaac cauccuggac uaucugauug augacggaaa uuccaaccgc     2220

aauuuuaugc agcucaucaa cgaugacgcu cugagcuuca aggaggagau ugccaaggcu     2280

caggugaucg gggaaaccga uaaucuuaac cagguugugu cugacauugc cggcuccccc     2340

gcgaucaaaa aggggauccu gcaaagccuc aaaauugucg acgagcuggu gaagaucaug     2400

ggccaccagc cugaaaauau ugugguugag auggccagag agaaccaguu cacaaaucag     2460

ggacggcgca acucucaaca gagacugaaa ggccuuaccg auuccaucaa ggaauuuggg     2520

agccagauuc ucaaagagca ucccgucgaa aauucccaac ugcagaacga ccggcuguuc     2580

cucuacuauc ugcagaaugg acgcgauaug uacacaggcg aggaacuuga caucgauuac     2640

cugucucagu augacaucga ucacauuauc ccccaggcuu uuauuaagga caacagcauc     2700

gauaauagag ugcucacguc cucuaaggag aaccggggga aaagcgacga ugugccuucc     2760

aaggacgucg ugcgcaaaau gaaaagcuac uggucuaaac ugcuguccgc caaacuuauu     2820

acccaaagaa aguucgacaa ccugacaaaa gcugagcggg gaggccucac cgaugacgau     2880

aaggccgggu uuaucaaacg ccagcugguu gaaacaagac agaucaccaa gcacguggcg     2940

cggauucucg acgagagauu caauacggaa accgaugaga acaauaaaaa gauccgccaa     3000

gucaagauug ugacacugaa aagcaaccuu guuucuaauu uucggaagga guucgaacug     3060

uauaaaguga gagagaucaa cgacuaccau cacgcccaug augcuuaucu caaugccguc     3120

auuggcaagg cucugcuggg aguguacccg cagcuugaac ccgaguuugu uuauggcgac     3180

uacccucacu uccacgggca uaaagaaaac aaggccaccg ccaagaaauu uuucuacucc     3240

aauaucauga acuucuuuaa gaaagaugac gugcgcacag auaagaaugg agagauuauc     3300

uggaaaaagg acgaacacau cagcaacauu aagaaagucc ugucuuaucc ccaggugaau     3360

aucgugaaga aagucgagga gcagaccggc ggguucagca aggaauccau ucucccuaaa     3420

ggaaacagcg auaagcugau cccccggaaa acgaagaagu uuuacuggga cacaaagaag     3480

uauggcgggu ucgacucucc gauuguugcu uacuccaucc ucgugaucgc ggauauugag     3540

aaaggcaaga gcaaaaagcu gaagaccgug aaagcccuug ucggagugac aaucauggaa     3600

aagaugaccu uugagagaga cccuguugcu uuccuggagc gcaaaggcua ucggaaugug     3660

caagaagaga acauuaucaa gcuccccaaa uacucccugu uuaagcugga aaacgggaga     3720

aagcgccuuc uggccucugc ccgggagcuc cagaaaggaa augaaauugu ccugccuaac     3780

caucucggca cccugcuuua ucacgcuaag aauauccaua aaguggauga gcccaagcac     3840

cuggacuacg uugauaaaca caaggacgag uucaaggaac ugcucgaugu ggucagcaac     3900

uuuuccaaaa aguacacacu ggccgagggg aaucuugaaa agaucaagga gcuguaugcg     3960

cagaacaaug gagaagaccu caaagagcug gcuucuagcu ucauuaaccu ccugacguuu     4020

accgccaucg gcgccccugc uacauucaag uuuuucgaua aaaauauuga cagaaagcgg     4080

uacaccucca caaccgagau ccuuaacgcc acgcugauuc aucaaucuau cacagggcug     4140

uaugaaaccc gcauugaucu caauaagcug ggcggagaca aaagacccgc ggcuaccaag     4200

aaagccggcc aggccaagaa aaagaaguaa ggacuagugu ccaccugucc cuccugggcu     4260

gcuggauugu cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga     4380

auu                                                                   4383


<210>  4835
<211>  3516
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4835
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugcccag acaacaaacc ugagcuacau ucucggacug      180

gaccuuggca ucgcguccgu ugggugggcu gugguggaga uuaaugaaaa cgaggauccg      240

aucggacuga uugacgucgg cgugcggauc uucgaacgcg ccgagguucc caagacgggg      300

gaaucucucg cccugagcag acggcuugcu cgcuccacca gacggcugau ccgcagacgg      360

gcccaucgcc uccugcuggc uaaaagauuu cucaagcggg aggggauucu gucuacaauc      420

gaucuugaga agggccugcc uaaucaagcc ugggaacucc gcguggcggg ccuggagaga      480

cggcuuagcg ccauugaaug gggagcuguc cugcuccacc ugaucaaaca ucgcggauau      540

cuguccaagc ggaaaaacga gucucagacc aauaacaagg agcucggcgc ccugcuuagc      600

gggguggcuc agaaucacca gcugcuccaa uccgacgauu acagaacacc cgccgaacug      660

gcgcuuaaaa aguucgccaa ggaggaagga cauauuagaa accagcgcgg cgcuuauacc      720

cacacauuua aucggcugga ccuccuggcc gagcugaacc uccuguucgc ucagcagcac      780

caauuuggga auccucauug caaagaacac auccagcagu acaugaccga gcuucugaug      840

uggcagaagc ccgcccucag cggagaggcc auucugaaaa ugcuuggcaa guguacgcau      900

gaaaagaacg aguucaaagc ugcgaaacac accuauucug ccgaaagauu uguuuggcug      960

acaaagcuca auaaccugcg cauccuggag gauggcgcug aacgggcccu caaugaggag     1020

gaaagacaac ugcuuaucaa ccacccuuac gagaagucca aacugaccua ugcccaggug     1080

cgcaagcucc uggggcugag cgaacaggcu auuuucaaac aucuucggua cucuaaggag     1140

aacgccgaau ccgcgacauu uauggagcuc aaggcuuggc acgccaucag aaaagcccug     1200

gagaaucaag gacugaagga caccuggcag gaucucgcua aaaagccgga ccugcuugau     1260

gaaauuggca cggccuucag ccuguacaaa acagacgagg auauccagca guaucucacc     1320

aacaaggucc ccaauucugu gauuaacgcu cugcuggugu cccuuaauuu ugacaaauuc     1380

aucgaacuca gccugaaguc ccugcgcaag auccucccuc ugauggagca agggaaacgg     1440

uacgaccagg ccugcagaga gauuuaugga caucacuacg gcgaagcgaa ccagaagaca     1500

ucucagcuuc ugcccgccau cccugcucaa gagauucgca aucccgucgu ucuccggacc     1560

cugagccagg ccagaaaagu gaucaacgcu auuauccggc aguauggguc cccggcccgc     1620

gugcauaucg aaaccggaag agagcugggc aagagcuuua aagaacggag agagauucag     1680

aagcaacagg aggauaaucg cacaaagcgg gaaucugcgg uccagaaauu caaggagcuu     1740

uucuccgacu uuagcucuga accuaaaagc aaggauaucc ucaaauucag acuguacgag     1800

cagcaacacg gcaagugucu guauuccggg aaagaaauua acauccaccg ccucaaugag     1860

aagggauacg uggagauuga ccaugcccug cccuuuucua gaacguggga ugacagcuuc     1920

aacaauaagg uucuugugcu ggcuuccgaa aaccagaaua aaggcaacca gaccccuuac     1980

gaguggcucc aagggaagau caacagcgaa cgguggaaaa auuuugucgc ccuggugcug     2040

ggaucucagu gcuccgcugc caagaaacag cgccuucuca cacagguuau ugaugacaac     2100

aaguucaucg auagaaaucu gaacgacacc cgguauaucg cccgcuuucu gagcaauuac     2160

auucaagaga accuccugcu ugugggcaag aauaaaaaga acgucuucac acccaauggg     2220

cagaucaccg cucugcucag aucucggugg ggccugauua aagcgcgcga aaacaauaac     2280

agacaccaug cccuggaugc uaucgugguc gccugugcca cgcccuccau gcagcagaag     2340

auuacacggu uuauccgcuu caaagaggug cacccuuaua agaucgagaa uagauacgaa     2400

augguugacc aagagagcgg agaaauuauc uccccgcacu uucccgagcc uugggcuuau     2460

uuccggcagg aggugaacau uagagucuuu gacaaucauc ccgauaccgu gcuuaaggaa     2520

augcugccug accgccccca ggccaaccac caguucguuc aaccucucuu cgugucucgg     2580

gcgccgacca gaaaaaugag cggccagggg cauauggaga caaucaaguc cgcuaaacgc     2640

cuggccgaag gaauuucugu ccuccggauc ccccugaccc agcuuaagcc uaaccugcuc     2700

gagaauaugg ugaacaaaga aagagagccc gcccuguacg cuggccugaa ggcccgccuu     2760

gcggaguuua aucaagaucc ugcuaaggcc uucgccacac ccuuuuauaa acaaggggga     2820

caacagguga aggcuauccg gguugaacag guccagaaaa gcggcgugcu ggugagagag     2880

aacaaugggg ucgccgacaa cgcuuccauu gugcgcacgg auguuuucau caagaauaac     2940

aaauuuuucc ucgugccgau uuacaccugg caggucgcca agggcauccu gccuaauaaa     3000

gcgauugugg cccacaagaa cgaagacgag ugggaagaga uggaugaggg agcuaaguuu     3060

aaauucagcc ucuuucccaa ugaccugguu gaacuuaaga ccaaaaagga guacuucuuu     3120

ggcuauuaca ucgggcugga ucgggccaca ggaaacaucu cucucaaaga acaugacggc     3180

gagauuucca aggggaagga uggaguguau agagucggcg ugaaacuggc ucugagcuuc     3240

gaaaaguacc agguugacga gcuugggaaa aaucggcaga ucugccgccc ccagcagaga     3300

cagccugugc ggaagcgccc ggccgccacc aaaaaggcug gccaagcgaa gaaaaagaaa     3360

uaaggacuag uguccaccug ucccuccugg gcugcuggau ugucucguuu uccugccaaa     3420

uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu                               3516


<210>  4836
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4836
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccgcg gcuuucaagc cgaaccccau uaauuacauc      180

cugggacucg acauuggcau cgccagcguu gggugggcca ugguggagau ugaugaagac      240

gagaacccua ucugccugau cgaucuugga gugcgggucu uugaacgcgc ugaggugccc      300

aaaaccggcg acucccuggc cauggcuaga cggcucgccc gcucuguuag acggcugaca      360

cgcagacggg cgcaucgccu ucugagagcc cggcgccucc ugaagagaga aggggugcug      420

caggcugccg auuucgacga gaauggacuc auuaagagcc ugccuaacac gcccuggcaa      480

cuucgggcug ccgcgcugga ucgcaaacuc accccucugg agugguccgc cguccuucug      540

caccucauca agcauagagg cuaucugucu cagcggaaaa augaaggcga gacagcugac      600

aaggaacugg gggcccuccu gaaaggagug gcugauaacg cccacgcccu ucagaccggc      660

gacuuuagaa caccggcuga gcuggcgcuc aauaaguucg agaaggaaag cgggcauauu      720

cgcaaccagc ggggagauua cucccacacc uuuucuagaa aagaccugca agccgagcuu      780

auccuccugu ucgaaaagca gaaagaguuu ggcaaucccc acguuagcgg gggacugaag      840

gaaggcauug agacacugcu caugacccag cgcccugcuc uguccggcga ugccgugcag      900

aaaaugcuug ggcauuguac guucgagccc gccgaaccua aggcugccaa aaacaccuau      960

acagcggagc gguuuaucug gcugaccaag cucaauaacc ugcgcauucu ugaacaagga     1020

agcgagagac cccugacaga caccgaacgg gcuacgcuca uggacgagcc guacagaaag     1080

ucuaaacuga cauaugccca ggcccgcaag cugcucggcc uggaggauac cgcuuucuuu     1140

aaagggcuuc gguacggaaa ggacaaugcc gaggcuucca cacugaugga aaugaaagcc     1200

uaucacgcga ucagcagagc ccucgaaaag gagggccuga aggauaaaaa gucuccucug     1260

aaccuuuccc ccgaacucca ggacgagauc gggaccgcuu ucagccuguu uaaaaccgau     1320

gaggacauua caggacgccu gaaggaucgg auccagccug aaauucucga ggcccugcuu     1380

aaacauaucu cuuucgacaa guucguccaa auuucccuga aggcucucag acggaucgug     1440

ccccugaugg aacagggcaa acgcuacgau gaggccugcg cggagaucua cggcgaccac     1500

uaugggaaga aaaauacgga agagaagauu uaccugcccc cuaucccggc cgaugaaauu     1560

agaaaccccg ugguccuucg ggcucucagc caggccagaa aaguuaucaa cggaguggug     1620

cgccgguaug gcuccccugc ucgcauucac aucgagaccg ccagagaagu cgggaagucu     1680

uuuaaggaca gaaaagagau cgagaagcgg caagaagaga aucgcaaaga cagagaaaag     1740

gccgcugcga aauuccggga guacuuuccc aacuucgugg gagaaccuaa gagcaaagau     1800

auucugaagc ugcgccucua ugagcagcag cauggcaagu gucuguacuc cgggaaagag     1860

aucaaucuug gcagacugaa cgaaaaggga uauguugaga uugaccacgc ccuccccuuu     1920

agccggacau gggaugacuc uuucaauaac aaagugcugg uccugggcuc cgaaaaucag     1980

aacaagggga aucaaacccc guacgaguac uuuaacggaa aagauaauag ccgcgaaugg     2040

caggaguuca aggcuagagu ggagacaucu cgguuuccuc gcagcaagaa acagagaauc     2100

cuucuccaga aguucgacga agauggcuuu aaagagcgga accugaauga caccagauau     2160

guuaaccgcu uccugugcca auuuguggcc gaucggauga gacucacggg gaagggcaaa     2220

aagcgcgucu ucgccuccaa uggacagauu acaaaccugc uucggggguu cuggggccug     2280

agaaaggugc gcgcugaaaa cgaccggcau cacgcccucg augcggucgu gguugcuugu     2340

ucuaccgugg ccaugcagca gaaaaucacc agauuugucc gcuacaagga gaugaaugcc     2400

uucgacggaa aaacaauuga uaaggagacc ggcgaagugc ugcaucagaa aacacacuuu     2460

ccccagccuu gggaguucuu ugcucaggaa guuaugaucc ggguguucgg gaagcccgac     2520

ggaaagccug aguuugaaga ggccgacacg cccgagaaac ugagaacccu ucucgcggaa     2580

aagcugagcu cccggccgga ggcuguccac gaauauguga caccucuguu cgugagccgc     2640

gcccccaaca gaaaaauguc uggccagggg cauauggaga ccguuaaguc cgccaaacgg     2700

cucgaugaag gagucagcgu gcugcgcgug ccccuuacac aacugaagcu caaagaccug     2760

gagaagaugg ucaauagaga gcgggaaccu aagcuguacg aggcucuuaa agccagacug     2820

gaagcucaca aggaugaccc ggccaaagcg uuugccgagc ccuucuauaa guacgauaaa     2880

gcuggcaacc gcacccaaca ggugaaggcc guucgggugg aacaagucca gaagacgggg     2940

guguggguua gaaaucauaa cggcaucgcu gacaaugcca ccauggugcg cgucgaugug     3000

uuugagaaag gagacaagua uuaccucguu ccuauuuacu cuuggcaagu ggccaaaggc     3060

auccugcccg aucgggcugu cgugcagggg aaggacgagg aagauuggca gcucauugac     3120

gauuccuuca acuuuaaauu cagccugcac ccuaaugacc uuguggaggu caucacaaag     3180

aaggcgagaa uguucggaua uuuugccucc ugccaccgcg gcaccgggaa cauuaauauc     3240

cggauccaug accuggauca caaaauugga aagaacggca uccucgaagg gauuggcguu     3300

aaaacagcuc ugucuuucca gaaguaccag aucgacgagc ugggaaaaga gauuagaccc     3360

ugucgccuua agaagagacc ucccgugcgg aaacgcccgg ccgccaccaa gaaagcuggc     3420

caggccaaga aaaagaagua aggacuagug uccaccuguc ccuccugggc ugcuggauug     3480

ucucguuuuc cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu           3594


<210>  4837
<211>  3600
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4837
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccaag uaccauguug gaauugacgu gggcaccuuc      180

agcguggggc uggcggcuau cgaggucgau gacgccggaa ugccgauuaa aacacucucc      240

cuggugucuc acauccauga uagcggccuu gaccccgaug aaauuaaguc cgccguuacg      300

cggcuggcuu cuagcgggau cgcccgcaga acccggcgcc ucuauagacg gaagcgcaga      360

cggcugcagc aacuugacaa auuuauccag cgccagggau ggccugugau ugagcuggaa      420

gauuacuccg acccccucua uccuuggaag gucagagcug agcuggccgc gucuuacauc      480

gccgaugaaa aagagcgggg cgagaagcug agcguggcuc uccgccacau ugccagacau      540

cggggcuggc gcaaccccua ugcuaaaguu uccagccugu accuuccuga cgggccgucu      600

gaugccuuca aggcgaucag agaagagauu aagcgggccu ccggacagcc cgugccugaa      660

acagcuaccg ucggccaaau ggugacacug ugcgagcucg ggacccugaa acuuagagga      720

gagggcgggg ugcugagcgc ccgccuccag cagucugacu augcucggga aauccaggag      780

auuuguagaa ugcaagaaau cggacaggag cuguaccgca agaucauuga ugucguuuuu      840

gccgccgaau cccccaaagg cagcgcuucu ucccgggugg gcaaggaccc ucugcagccc      900

gggaaaaaua gagcgcucaa ggccagcgac gcuuuccagc gcuaccggau cgccgcccug      960

auuggaaacc uuagagugcg cgucgauggc gagaaacgga uccuguccgu ggaggaaaag     1020

aaucucguuu uugaccaccu ggugaaccuu acaccgaaga aagagccuga augggucacc     1080

auugcugaga uccuggggau cgauagagga caacucauug gcacggccac caugacagac     1140

gauggggaac gcgcgggagc ucggcccccu acccacgaca caaauagauc uaucgugaac     1200

agccggauug cuccccuggu ugauuggugg aagaccgccu ccgcccugga gcagcaugcc     1260

auggugaaag cucucagcaa ugccgagguc gacgauuucg acucucccga aggcgcgaag     1320

gugcaggccu uuuucgcuga ucuggacgac gauguccacg ccaaacuuga cucccugcau     1380

cucccugugg gccgcgcugc cuauagcgag gauacgcugg uuagacuuac acggagaaug     1440

cugucugacg ggguggaucu cuacaccgcg cgccugcaag aauuuggaau cgagccgagc     1500

uggacacccc cuacaccccg gauuggcgaa ccugucggga accccgccgu ggacagaguu     1560

cugaagaccg ugucccgcug gcucgagucu gcuaccaaga cguggggagc cccggagaga     1620

gucaucaucg aacacgugcg ggagggcuuc gugaccgaaa aacgcgcuag agagauggau     1680

ggggacaugc ggcgcagagc cgcccggaau gcuaagcugu uucaggagau gcaggaaaaa     1740

cuuaacguuc agggcaagcc uagccgcgcg gaucugugga gauaucaauc cguccagcgg     1800

cagaauugcc agugugccua cugcggaagc cccauuacau ucucuaacuc cgagauggac     1860

cacaucgugc cucgcgcugg ccaagggagc accaauacaa gagaaaaccu cguggccguc     1920

ugucaucggu gcaaucaguc uaaaggaaac acccccuuug ccauuugggc uaagaacacg     1980

uccaucgagg gcgugagcgu uaaggaagcc guggagagaa cacgccacug ggucaccgau     2040

accgggaugc gguccacaga cuucaaaaag uucaccaaag cggugguuga gagauuucag     2100

cgcgcuacaa uggaugaaga gauugacgcc cggucuaugg aaagcguggc cuggauggcu     2160

aaugagcuga gaucccgcgu cgcccagcau uucgcgucuc acggaacgac cgugcggguu     2220

uauagaggca gccugacagc ugaagcccgc cgggccuccg ggaucagcgg caagcugaaa     2280

uuuuucgacg gagugggcaa gucuagacuc gaucggcgcc aucacgcuau ugacgccgcu     2340

gucaucgccu uuaccuccga uuacguggcg gagacacugg ccgugagaag caaccugaag     2400

caaucucagg cucaccggca ggaggccccu caguggcgcg aauuuaccgg gaaagacgcu     2460

gagcauagag ccgccuggcg ggucuggugu caaaagaugg aaaaacucuc cgcucugcuu     2520

acggaggauc ugagagacga ucgcguugug gugaugagca auguccggcu cagacuggga     2580

aacggcucug cgcacaagga aaccaucggg aaacugagca aggugaaacu uuccagccag     2640

cucucuguuu ccgacauuga uaaggccagc uccgaggcuc uguggugcgc ccugacacgc     2700

gagcccggau uugacccgaa ggaaggccuc ccugccaauc ccgagcggca uaucagagug     2760

aacgggaccc acgucuaugc uggcgauaau auuggacugu uccccguguc ugccggcagc     2820

aucgcgcuuc gcgggggaua cgcugaacug ggcuccucuu uucaccaugc ccggguuuac     2880

aaaauuacaa gcgggaagaa accugccuuc gcuaugcuca gaguguauac caucgaccug     2940

cugccguacc gcaaccagga ucuuuucucc gucgagcuca agccccaaac gaugucuaug     3000

agacaggccg agaaaaagcu gcgggacgcg cuggcuacag gaaaugccga auaucucggc     3060

uggcuggugg uugacgauga gcuugugguc gacaccagca agaucgccac agaucaggug     3120

aaagcugugg aagccgagcu ggggaccauu cgcagauggc gggucgacgg cuucuuuucc     3180

ccuagcaagc uccgccugag accccugcag augucuaaag aaggaaucaa gaaagagucc     3240

gcuccugagc uuagcaagau uaucgaucgg cccggcuggc ucccugccgu uaacaagcug     3300

uucucugacg ggaaugugac cguggucaga cgcgauuccc ugggacgggu gagacucgaa     3360

agcacagcgc accuucccgu uacguggaaa gugcaaaagc gcccggccgc uaccaaaaag     3420

gccggccagg cuaaaaagaa gaaauaagga cuagugucca ccugucccuc cugggcugcu     3480

ggauugucuc guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3600


<210>  4838
<211>  3300
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4838
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccgcg cggauucugg cuuucgacau cggaauuagc      180

uccaucggcu gggccuuuuc ugagaacgau gaacucaagg acugcggggu ucgcauuuuc      240

accaaagugg agaauccgaa gacaggagaa agccuggccc uucccagacg gcuggcucgc      300

uccgccagaa agcggcucgc ucgcagaaaa gcccggcuga accaucuuaa gcaccugauc      360

gcgaaugagu uuaaacucaa cuacgaagau uaucagucuu ucgacgagag ccuggccaag      420

gcuuacaaag gcucccugau cucuccuuau gagcuccgcu uuagagcccu gaaugaacuu      480

cugagcaagc aagauuucgc ucgggugauu cuccauaucg ccaagcgcag aggguacgac      540

gauauuaaaa acuccgacga uaaggagaaa ggagcgaucc ugaaggccau uaaacagaau      600

gaagagaaac uugcuaacua ucagagcguc ggcgaguacc uguauaaaga auacuuucag      660

aaguucaagg aaaauucuaa agaauuuacg aacgugcgga auaagaaaga guccuacgaa      720

cgcuguaucg cccagagcuu ccucaaggac gagcugaaac ugauuuuuaa gaagcaaaga      780

gaguucggcu uuucuuucuc caaaaaguuc gaagaggaag uucucagcgu ggcuuuuuau      840

aaacgggccc ugaaggauuu cucucaccuu gucgggaacu gcuccuuuuu caccgacgag      900

aaaagagccc ccaagaauag cccucuggcu uuuauguucg uggcgcucac acgcaucauc      960

aaccugcuua auaaccugaa gaacaccgaa ggcauucucu acacaaaaga ugaccugaau     1020

gcccugcuca acgagguucu gaagaaugga acccuuacau auaaacagac caagaaacug     1080

cucgggcugu ccgacgauua cgaguuuaag ggagaaaagg gcacguauuu caucgaguuu     1140

aaaaaguaca aagaguucau uaaggcucuu ggggagcaua accugucuca ggacgaucuc     1200

aaugaaaucg ccaaagacau uacccugauc aaggaugaga ucaaacugaa gaaggcccuc     1260

gcuaaauaug accugaacca aaaucagauu gauagccuuu ccaagcugga guuuaaagac     1320

caccucaaca ucagcuucaa ggcccugaaa cuggugacac cccuuaugcu cgaaggaaag     1380

aaguacgaug aggcguguaa ugaacugaac cugaaagucg cuauuaauga ggacaagaaa     1440

gauuuucucc cugccuucaa cgagaccuac uauaaggacg aagugacaaa uccggugguc     1500

cugcgggcca ucaaagagua cagaaagguu cuuaacgcuc ugcucaagaa auauggcaag     1560

gugcacaaaa uuaauaucga acuggcccgc gaggugggca agaaccauuc ucagcgggcu     1620

aaaaucgaaa aggagcagaa cgagaauuac aaggccaaaa aggacgcgga acuggagugc     1680

gaaaaacuug ggcucaagau uaacuccaaa aauauccuga agcugagacu cuucaaagag     1740

caaaaggaau uuugugccua uagcggagag aagauuaaaa ucucugaucu gcaggacgag     1800

aagaugcuug aaauugauca caucuacccc uauagccgcu ccuucgacga uucuuacaug     1860

aacaaagucc ugguguuuac caagcagaau caagagaaac ucaaccagac gccuuucgag     1920

gcuuuuggca augacagcgc caaguggcag aagauugaag uucuggcuaa aaaccugccc     1980

acaaagaaac agaagcggau ccuugauaaa aauuacaagg acaaggaaca aaagaacuuc     2040

aaggauagaa aucucaacga cacccgcuau aucgcccggc uggugcugaa uuacacaaaa     2100

gauuaucucg acuuucugcc ucuuuccgau gacgagaaca ccaagcugaa ugacacccag     2160

aaagggagca agguccaugu ggaggcgaag ucuggaaugc ucacauccgc ccugagacac     2220

acguggggcu ucagcgcuaa agaucgcaac aaccaucugc accacgccau ugacgcuguu     2280

aucauugccu acgccaauaa cucuaucgug aaggcuuuuu ccgauuucaa aaaggaacag     2340

gagagcaauu ccgcggaacu uuaugccaaa aagauuucug agcucgacua caagaacaaa     2400

cggaaguuuu ucgagcccuu uagcggguuc agacagaaag uccuggauaa gaucgacgaa     2460

aucuucgugu ccaaaccgga gcggaagaaa ccuucuggag cucugcauga agagaccuuu     2520

cgcaaggaag aggaguucua ucaaagcuac ggcggcaagg aagggguccu caaagcccug     2580

gagcuuggaa agauuagaaa agugaauggc aagaucguua aaaacgggga uauguuucgg     2640

guggacauuu ucaagcacaa gaaaacaaau aaguuuuacg ccguccccau cuauaccaug     2700

gauuucgcuc ugaaagugcu cccuaacaag gccguugcga gauccaaaaa gggagaaauu     2760

aaggacugga uccugaugga ugagaauuac gaauuuugcu ucagccugua uaaagacucu     2820

cuuauccuga uucagacaaa ggauaugcag gagcccgagu uuguguacua uaacgcuuuc     2880

accuccagca cggucucucu caucgugucc aaacaugaca auaaguuuga aacacugagc     2940

aaaaaccaga agauucucuu caagaaugcc aacgagaaag aagugaucgc caagucuauu     3000

ggcauccaaa aucugaaagu uuucgagaag uacaucguca gcgcucuugg ggaagugacc     3060

aaagccgagu uucgccagcg ggaggacuuc aagaaaaaga gacccgcggc uaccaagaaa     3120

gccggccagg ccaagaaaaa gaaauaagga cuagugucca ccugucccuc cugggcugcu     3180

ggauugucuc guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu     3300


<210>  4839
<211>  3711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4839
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccagc gaccugguuc ucggacugga uauuggcauc      180

ggguccgugg gagugggcau ucuuaacaag gucaccgggg agaucauuca uaaaaauucu      240

cggaucuucc cggcggcuca ggccgaaaac aaucuggugc gcagaacaaa ccggcaagga      300

cgcagacucg cccggcgcaa gaagcacaga cggguucgcc ugaauagacu uuuugaggaa      360

agcggccuga ucacggacuu caccaaaauu uccaucaacc ucaaucccua ccagcugcgg      420

gugaagggcc ugacagauga gcucucuaac gaggagcugu uuauugcucu uaaaaauaug      480

gucaagcauc gcgggaucag cuaucuggac gaugccuccg acgauggaaa cucuagcgug      540

ggcgacuacg cucagauugu uaaagaaaau uccaagcagc ucgaaaccaa gacaccuggg      600

caaauccagc uggagagaua ucagaccuac ggacagcuuc ggggcgauuu cacaguggaa      660

aaagacggga agaaacaccg ccugauuaac gucuuuccca ccagcgccua uagaucugag      720

gcgcuccgga uccugcaaac gcagcaggag uucaauccuc agaucaccga ugaauuuauu      780

aacagauacc uggagauccu cacaggaaag cgcaaauauu accauggccc cggcaaugaa      840

aagucccgga ccgacuacgg gagauaucgc acaagcggag agacccugga uaacauuuuc      900

ggcauccuua uugggaaaug cacguuuuac ccugacgagu uccgggccgc uaaggccucu      960

uauacagcuc aagaauuuaa ccugcucaau gaccugaaca aucuuaccgu gccgacagag     1020

accaagaaac uguccaagga acagaaaaac cagaucauca auuacgugaa gaacgagaaa     1080

gccaugggac ccgcgaaacu cuucaaguau auugccaaac ugcugagcug ugaugucgcu     1140

gacaucaagg gcuacagaau ugauaaaucu gggaaggccg aaauccacac cuuugaggcu     1200

uaucgcaaaa ugaagacacu cgaaacgcug gacauugagc aaauggaucg ggagacccuu     1260

gacaagcugg ccuacguucu cacacugaau accgaaagag agggaaucca ggaagcccuu     1320

gagcacgagu ucgcugaugg cuccuucagc cagaaacagg uggacgaacu ggugcaauuu     1380

cgcaaggcga acuccucuau cuucggcaaa ggguggcaua auuuuagcgu caagcucaug     1440

auggagcuga uuccugaacu guacgagaca uccgaggaac agaugaccau ccucacgcgg     1500

cugggaaaac agaagacaac cucuucuucc aacaagacca aauauauuga ugagaaacuu     1560

cugacagaag agaucuacaa ucccgugguu gccaagagcg ugagacaggc uauuaagauc     1620

gucaacgccg ccauuaaaga auauggcgac uucgauaaua ucgugaucga gauggcucgc     1680

gaaaccaacg aggacgacga gaagaaagcc auucaaaaga uccagaaggc gaauaaagau     1740

gaaaaggacg cugccaugcu caaagccgcu aaccaguaca acgggaaggc cgagcugccu     1800

cacucuguuu uucauggaca caaacagcug gcuacaaaga uucggcuuug gcaccaacag     1860

ggcgaaagau gccucuauac ggggaagacc aucagcauuc augaucugau caauaacucc     1920

aaucaguucg agguggacca cauccugccc cucucuauua cauuugauga cagccuggcc     1980

aacaaagucc uuguguacgc gaccgccaau caggaaaagg gccaacggac accguaucag     2040

gcucuggauu ccauggacga ugccuggagc uuccgcgagc ucaaggcuuu ugucagagag     2100

ucuaagaccc uguccaacaa aaagaaggaa uaccugcuua cggaggaaga caucagcaaa     2160

uucgaugugc ggaagaaauu uauugagaga aaucucguug acacccgcua cgccucucgg     2220

gugguccuga acgcgcugca ggaacauuuc agagcccaca agaucgauac aaaagugucc     2280

guugugcgcg gacaauuuac cagccagcuc agacggcauu ggggcauuga gaagacacgc     2340

gacaccuauc accaccaugc ugucgacgcc cugaucaucg cugccuccuc ucagcuuaau     2400

cuguggaaga aacagaagaa cacgcucgug agcuacuccg aggaucaacu gcuggacauu     2460

gaaacagggg agcuuaucuc ugaugacgaa uauaaagaga gcguguucaa ggccccuuac     2520

cagcacuucg uugauacccu caaauccaag gaguuugaag acagcauucu guucucuuau     2580

caggucgauu ccaaauuuaa uagaaagauc agcgacgcua caauuuacgc gacccggcag     2640

gccaaggugg gaaaagauaa ggcugacgag accuaugugc ugggcaaaau caaggauauc     2700

uacacacaag acggguacga ugccuucaug aaaauuuaua agaaggacaa aucuaaguuu     2760

cucauguacc gccaugaccc ccagacguuc gaaaaaguca ucgagccuau ucuggaaaac     2820

uaucccaaua agcagaucaa cgagaaagga aaggaggugc ccuguaaucc uuuucuuaag     2880

uacaaagagg aacacggcua uauuagaaag uacuccaaaa aggggaacgg cccggaaauc     2940

aaaagccuga aguauuacga uucuaagcuc ggaaaccaca uugacaucac ccccaaagau     3000

agcaauaaca agguugugcu gcaguccguc agcccuuggc gggccgacgu guacuucaau     3060

aaaacaaccg gcaaguauga gauccugggg cuuaaauacg cugaucucca auuugaaaag     3120

ggaacaggca ccuauaagau uucucaggag aaauacaacg acaucaagaa aaaggagggg     3180

guugauuccg acagcgaguu caaauuuacg cuguauaaga augaucugcu ccuggugaaa     3240

gacacagaaa ccaaggaaca gcaacuuuuc cgcuuucugu ccagaaccau gcccaagcag     3300

aaacauuacg ucgagcucaa gccuuaugau aaacagaagu ucgaaggagg cgaggcccug     3360

auuaaagugc uggggaacgu ugcgaauagc ggccagugca agaagggacu uggcaaaagc     3420

aacaucucca uuuacaaggu gcggacagac guccugggga aucaacacau caucaaaaac     3480

gagggagaca agcccaaacu cgauuucaag cgcccggcug ccaccaagaa agccggccag     3540

gcuaagaaaa agaaauaagg acuagugucc accugucccu ccugggcugc uggauugucu     3600

cguuuuccug ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u              3711


<210>  4840
<211>  4269
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4840
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccacc caguucgagg gauuuacaaa ccuguaccaa      180

guuagcaaga cgcuccgguu cgaacugauu ccgcagggca aaacccuuaa gcauauccag      240

gagcaggggu uuauugaaga ggacaaggcg cgcaaugauc acuauaaaga acugaagccc      300

aucauugaca gaaucuacaa aacauaugcu gaucaaugcc uccagcuggu gcagcuugac      360

ugggagaacc uguccgccgc caucgauucu uaccggaagg agaaaaccga agagacacgc      420

aaugcucuca uugaagaaca ggccaccuau agaaacgcua uccaugacua cuucauugga      480

cggacagaua aucugaccga cgccaucaac aagcgccacg cggagauuua uaagggccug      540

uuuaaagccg aacucuucaa ugggaaggug cugaaacaac uuggaacggu caccacaacc      600

gagcaugaaa acgcucugcu cagauccuuu gauaaguuca caaccuacuu uagcggcuuc      660

uacgagaauc ggaaaaacgu guuuucugcc gaagacauca gcacggcuau uccucaccgc      720

aucguucagg auaauuuccc caaguuuaaa gagaacuguc acaucuucac aagacugauu      780

accgccgugc cuucccuucg ggagcauuuc gaaaauguca agaaggcgau cggcauuuuu      840

gugucuacaa gcaucgagga aguuuucucc uuucccuucu auaaccagcu gcucacccag      900

acccaaauug accuguacaa ucagcugcuc gggggaauca gccgcgaggc cggcacagag      960

aaaaucaagg ggcugaacga ggugcuuaac cuggcuauuc agaaaaauga ugagacggcc     1020

cacaucauug cuucucuccc ucauagauuu aucccgcugu ucaagcaaau ucuuuccgac     1080

cggaacaccc ugagcuuuau ccucgaagag uucaaaucug acgaagaggu cauccagucc     1140

uuuugcaagu auaagacacu gcugcgcaau gaaaacgugc ucgagaccgc cgaggcccug     1200

uucaaugaac uuaacagcau ugaucugaca cacaucuuua uuucucacaa aaagcucgag     1260

accaucucca gcgcucugug ugaccauugg gauacgcuua gaaaugcgcu guacgaacgg     1320

agaauuuccg agcucacagg aaaaaucacc aagucugcca aagagaaggu gcagcgcagc     1380

cugaagcacg aagacauuaa ccugcaggag aucaucuccg cugccggcaa agaacucagc     1440

gaggccuuca agcagaaaac cucugaaauu cugucccaug cucacgccgc gcuugaucaa     1500

ccccugccua caacccucaa gaaacaggag gagaaggaaa uccuuaagag ccagcuggac     1560

ucucuucucg ggcuguauca ucugcucgau ugguuugcug ucgacgagag caaugaaguu     1620

gaucccgagu ucuccgcccg gcugacagga auuaaacuug aaauggagcc uucucugagc     1680

uucuacaaca aggccagaaa uuaugcuacg aaaaagcccu acuccgugga gaaauuuaag     1740

cucaacuucc aaaugccgac ccuggccagc ggcugggacg ugaauaaaga aaagaacaau     1800

ggcgcuaucc uguuugucaa gaacgggcuu uacuaucucg gaauuaugcc uaaacagaag     1860

ggccgcuaca aagcccuguc uuucgagccc acagaaaaga ccuccgaggg guuugauaaa     1920

auguauuacg acuauuuccc ugacgcggcc aagaugaucc ccaagugcag cacacagcug     1980

aaagcuguga ccgcccacuu ucagacgcac accacaccca uccuccuguc uaacaauuuc     2040

auugaaccuc uugagaucac caaggagauu uacgaucuga acaauccgga aaaagagccc     2100

aagaaauuuc aaacagcuua ugccaagaag accggagacc agaaaggcua ccgggaagcg     2160

cucuguaagu ggaucgauuu cacgagagac uuucugucca aauacacaaa gaccacaagc     2220

auugaucugu ccucucuucg cccuagcucc caguauaaag accucgggga guacuaugcc     2280

gagcugaacc cccugcucua ccauaucucu uuccagcgga ucgcugaaaa ggagauuaug     2340

gaugccguug aaaccggaaa gcuguaucuu uuucaaaucu acaauaaaga cuucgcuaag     2400

ggccaccaug gcaaaccuaa ccugcacacc cucuauugga cagggcuguu cagccccgag     2460

aaucuggcca agacguccau uaaacuuaac ggacaggccg aacucuuuua cagaccuaag     2520

agccgcauga aacggauggc ucacagacug ggcgagaaga ugcugaauaa gaaacucaag     2580

gaucagaaaa ccccgauccc cgacacacug uaccaagagc uuuaugauua cgugaaccau     2640

cgccugucuc acgaccucuc cgaugaagcg cgggcccugc ugccuaaugu cauuaccaag     2700

gaggugagcc augaaaucau caaagacaga cgguucacau cugacaaguu uuucuuucac     2760

guucccauua cccuuaacua ucaggcugcc aauuccccua gcaaguucaa ccagcgcgug     2820

aaugccuacc ugaaagagca ucccgaaacg ccgaucauug ggaucgauag aggagagcgg     2880

aaccucauuu auaucacagu cauugacucu accggcaaga uccuggagca gagaagccuc     2940

aacaccaucc aacaguuuga uuaccagaaa aagcuggaca aucgcgaaaa agagcgggug     3000

gcugccagac aggcgugguc cgucgugggg acaauuaagg aucuuaagca aggcuaucug     3060

agccagguua uccacgaaau uguggaccuc augauccacu accaggcugu cgugguucug     3120

gagaaccuga auuucggauu uaaaucuaag cgcaccggca uugccgaaaa agccguguac     3180

cagcaauucg agaagaugcu uaucgauaaa cugaacugcc ucguccugaa ggacuauccu     3240

gcugagaagg uugggggagu gcucaauccc uaccagcuga cagaucaguu uacguccuuc     3300

gccaaaaugg gcacccaaag cggguuccug uuuuaugugc ccgcgccuua cacauccaag     3360

aucgacccgc ugaccggauu cgucgauccc uuugugugga aaacaauuaa gaaccaugaa     3420

ucuagaaaac acuuccucga gggcuuugac uuccugcauu augaugugaa gaccggggac     3480

uuuauccugc acuucaagau gaaucggaac cuuagcuuuc agcgcggccu gccuggauuc     3540

augcccgcuu gggacauugu cuuugagaaa aaugaaacgc aguucgaugc caagggcacc     3600

ccuuuuaucg ccgggaaaag aauugugccc guuaucgaga accaucgguu cacaggacgc     3660

uacagagacc ucuauccugc uaaugaacug aucgcccucc uggaggaaaa gggcauugug     3720

uuccgggaug gguccaacau ccuucccaaa cugcuggaga augacgauuc ucacgcuauu     3780

gacaccaugg ucgcccucau ccgcagcgug cugcagauga gaaacuccaa ugcggccaca     3840

ggagaagauu acauuaacuc uccgguucgg gaccuuaacg gcguguguuu ugauagccgc     3900

uuccaaaauc cugaguggcc cauggacgcu gaugccaacg gggcuuacca caucgcccug     3960

aagggccagc uccugcucaa ucaucugaaa gaguccaagg accuuaagcu gcagaacgga     4020

auuagcaauc aggacuggcu ggccuauauc caagaacuca gaaacaaacg gccugcugcg     4080

accaagaaag ccggccaggc uaagaaaaag aaguaaggac uaguguccac cugucccucc     4140

ugggcugcug gauugucucg uuuuccugcc aaauaaacag gaucagcgcu uuacagaucu     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4260

aaaagaauu                                                             4269


<210>  4841
<211>  4248
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4841
gggagagucc gcucagccag guugcagaag cggcuuagcg uguguccuaa ucuucucucu       60

gcguguaggu aggccugugc cgcaaacaag cuuaccaugg cccccaagaa aaagagaaaa      120

gugggcaucc acgggguccc ugcugccagc auuuaccagg aguucguuaa caaguauucc      180

cugucuaaaa cccuccgguu ugaacugauc ccgcaaggaa agacacuuga gaauauuaag      240

gcgcgcggcc ugauccucga cgaugaaaag agagcuaagg acuacaaaaa ggccaaacag      300

auuaucgaua aguaucauca guucuuuauc gaggaaauuc ugagcuccgu gugcaucucu      360

gaggaccuuc ugcagaacua cagcgaugug uauuucaagc ucaaaaaguc cgacgaugac      420

aaucugcaaa aagauuuuaa gucugccaaa gacacgauua agaaacagau cagcgaguac      480

auuaaggauu ccgaaaaguu caaaaaccug uuuaaucaga accucaucga cgcuaagaaa      540

gggcaggaga gcgaucugau ucuuuggcug aagcaaucua aagacaaugg aaucgaacuc      600

uucaaggcca acuccgacau caccgauauu gacgaggcuc uggagaucau uaagagcuuu      660

aaaggcugga caaccuauuu caagggguuu cacgaaaauc ggaaaaacgu cuacucuucc      720

aaugauaucc ccacaagcau uaucuaccgc aucguggacg auaaccuucc uaaguuccug      780

gagaauaaag ccaaguauga aucucucaag gacaaagcgc ccgaggccau uaacuacgaa      840

cagaucaaga aagaucuggc ugaggagcug accuuugaca uugauuauaa gacauccgaa      900

guuaaucaga gaguguucag ccucgacgag gucuucgaaa ucgccaacuu uaacaauuac      960

cugaaccagu ccggaauuac caaauucaau acgaucaucg gcggcaaguu ugugaacggg     1020

gagaauacca agcggaaagg aauuaacgaa uauaucaauc uuuacucuca acagauuaac     1080

gauaagacac ugaaaaagua uaaaaugagc guucucuuca agcagauccu guccgacacc     1140

gagagcaaau cuuuugugau ugacaaacuu gaggaugacu ccgaugucgu gacaaccaug     1200

cagagcuucu acgaacaaau cgcugccuuu aagacggugg aggaaaaauc uauuaaggag     1260

acacugagcc uccuguucga cgaucugaaa gcgcagaagc ucgaccuguc caaaaucuac     1320

uuuaagaaug auaagucucu uaccgaccug agccagcaag ucuucgauga cuauuccguu     1380

aucggcacag ccgugcucga auacauuacc cagcagaucg cuccuaaaaa ccuggauaau     1440

cccagcaaga aagagcagga gcuuauugcc aagaaaaccg aaaaggcuaa guaucugucu     1500

cucgagacaa ucaaacuggc ccuggaagag uuuaacaagc aucgcgacau ugauaaacaa     1560

uguagauucg aggaaauccu cgccaauuuu gcugcgaucc cuaugauuuu cgacgagauc     1620

gcccagaaca aggacaaucu ggcucagauu uccaucaaau accagaacca agggaagaag     1680

gaucuucugc aggccagcgc cgaagacgau gugaaagcua uuaaggaccu ccuggaucag     1740

acgaacaauc ugcuucacaa acucaagauc uuccauaucu cucaguccga ggacaaagcc     1800

aacauucugg auaaggacga acacuuuuau cuggucuucg aggagugcua cuuugaacuc     1860

gcgaauaucg ugccgcugua uaacaaaauu cggaauuaca ucacccaaaa gcccuacagc     1920

gaugagaagu ucaaacuuaa cuuugaaaau uccacacugg cuaacggaug ggacaagaau     1980

aaagagccug auaacaccgc cauucucuuc aucaaggacg auaaauauua ccugggcguu     2040

augaauaaga agaacaauaa aaucuuugac gacaaggcca uuaaagaaaa caagggggag     2100

ggauauaaaa agaucgugua caagcugcuu cccggcgcua auaaaaugcu cccuaagguc     2160

uucuuuucug ccaaaagcau uaaguucuau aaccccuccg aggauauccu gcgcauuaga     2220

aaccacucua cacauaccaa aaauggcagc ccgcagaagg gguacgaaaa guuugaguuc     2280

aacaucgaag acugucggaa auuuauugau uucuauaagc aguccaucag caaacacccu     2340

gaguggaagg acuucggauu ucgcuucucu gauacgcaaa gauacaauuc caucgacgaa     2400

uuuuaccggg agguggagaa ccagggcuau aaacugacau ucgaaaauau uagcgagucu     2460

uacaucgauu ccguugugaa ccaggggaag cucuaucugu uucagauuua caauaaggac     2520

uucagcgcuu auucuaaagg acgccccaac cuucauaccc uguacuggaa ggcccucuuu     2580

gaugaaagaa aucugcaaga cgucguguau aaacugaacg gcgaggcgga gcuuuucuac     2640

cggaagcaga gcaucccuaa aaagauuacc caccccgcca aagaagcuau cgccaauaag     2700

aacaaggaua aucccaaaaa ggaguccguc uuugaauacg accucaucaa agacaagaga     2760

uucacagagg auaaauuuuu cuuccacugc ccuauuacca ucaacuuuaa gagcucuggg     2820

gcuaauaagu ucaacgacga aauuaaccug cugcucaaag agaaggccaa ugaugugcau     2880

auccugucca uugaccgcgg agagcggcac cuugcguauu acacacuggu ugauggcaaa     2940

ggcaacauca ucaagcagga cacguuuaau auuaucggga acgauagaau gaaaaccaau     3000

uaucaugaca agcucgccgc uauugaaaag gaucgcgaca gcgcccggaa agauuggaag     3060

aaaaucaaca auauuaagga gaugaaagaa ggauaccugu cccagguggu ccacgagauc     3120

gcuaagcugg ugauugaaua uaacgccauc guuguguucg aggaccuuaa uuuuggcuuc     3180

aagagagggc gcuuuaaagu cgagaagcaa guguaccaga aacucgaaaa gaugcugauc     3240

gagaaacuga acuaucucgu guucaaggau aaugaauuug acaaaacagg aggcguucug     3300

cgggccuacc agcuuaccgc uccguucgag acauuuaaga agauggggaa acagaccggc     3360

auuaucuacu auguccccgc gggauucacg ucuaagauuu guccugugac cggcuuugug     3420

aaccaacugu accccaaaua ugaaagcguc uccaagucuc aggaguucuu cagcaaauuu     3480

gacaagaucu gcuacaaucu cgauaagggg uauuucgagu uuuccuucga cuacaaaaac     3540

uuuggagaua aggccgcuaa aggcaagugg acaauugccu cuuucgggag cagacugauc     3600

aauuuucgca acuccgacaa aaaucauaac ugggauaccc gggaagugua uccuacaaag     3660

gagcuggaaa aacuucugaa agacuacagc aucgaguacg gacacggcga guguauuaag     3720

gccgcuaucu gcggggaauc ugauaaaaag uucuuugcca aacucaccuc cguucugaac     3780

acgauucucc agaugagaaa uagcaagaca ggcaccgagc uggacuaucu uaucucuccc     3840

guggcggaug ucaacggaaa uuucuuugac ucccgccaag cuccgaagaa caugccucag     3900

gaugccgacg ccaauggcgc uuaccacauu gggcugaaag gacucaugcu gcugggccgg     3960

aucaagaaca aucaggaagg gaaaaagcug aaccuuguga ucaaaaauga ggaauauuuc     4020

gaguuuguuc agaacagaaa uaacaagcgg cccgccgcga caaagaaggc uggacaagcc     4080

aagaaaaaga aauaaggacu aguguccacc ugucccuccu gggcugcugg auugucucgu     4140

uuuccugcca aauaaacagg aucagcgcuu uacagaucua aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaauu                  4248


<210>  4842
<211>  4433
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4842
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccga caagaaauac agcauuggac uggauaucgg caccaacucc guuggguggg      180

cggugauuac agacgaguau aaggugccgu cuaagaaauu caagguccuc ggaaauacgg      240

aucggcauag caucaaaaag aaccugauug gcgcucugcu guuugacucc ggggaaaccg      300

ccgaggccac acgccucaaa agaaccgcuc ggcgcagaua cacacggcgc aagaauagaa      360

ucugcuaucu gcaggaaauc uucucuaacg agauggccaa gguggaugac agcuuuuucc      420

accggcuuga agaguccuuu cugguugagg aagauaaaaa gcaugagcgc caccccauuu      480

ucggaaauau cguggacgaa gucgcuuacc augagaaaua uccuaccauu uaccaccuca      540

gaaagaaacu gguggauucu acagacaagg ccgaucugcg gcucaucuau cuggcgcuug      600

cccacaugau uaaauuucgc ggccauuucc ugaucgaggg cgaccucaac cccgauaaua      660

gcgacguuga caagcuguuu auucaacuug ugcagaccua caaccagcug uucgaagaga      720

auccuaucaa cgcuuccggg gucgaugcca aggcuauccu cagcgccaga cugucuaaau      780

cccggcgccu ggaaaaucuc auugcgcagc ugcccggaga gaagaaaaac ggccuuuuug      840

ggaaucugau cgcccucagc cugggacuua cgccuaacuu caagucuaau uuugaccugg      900

cugaagaugc caaacuccaa cuguccaagg acaccuacga ugacgaucug gacaaccucc      960

uggcucagau uggcgaucag uaugccgacc uuuuccuggc cgcuaagaac cucagcgaug     1020

cgauccugcu uucugacauu cugagaguga auacagagau caccaaagcc ccgcucuccg     1080

cuagcaugau caagcgguac gacgagcacc aucaggaucu gacacugcuc aaagcccugg     1140

ugagacaaca gcuucccgaa aaguauaaag agauuuucuu ugaccagucc aagaacgggu     1200

acgccggaua uaucgauggc ggcgcuucuc aggaagaguu cuacaaguuu auuaaaccua     1260

uccuggaaaa gauggacggg accgaggagc uccuggucaa acugaaucgc gaagaucuuc     1320

uccggaagca aagaacguuc gacaacggaa gcauucccca ccagauccac cugggcgagc     1380

ugcaugccau ccuccgccgg caggaagauu uuuauccuuu ccugaaagac aauagagaga     1440

agauugaaaa gauccuuaca uuucgcauuc ccuacuacgu ugggccgcug gcgcggggaa     1500

acuccagauu cgcuuggaug acccgcaaaa gcgaggagac aaucaccccu uggaauuuug     1560

aagagguggu ggauaagggc gccucugccc aauccuucau ugaacggaug accaacuuug     1620

acaaaaaucu ccccaacgag aagguccugc cuaaacacag ccugcuuuau gaguacuuca     1680

caguguauaa ugaacucacg aagguuaaau acgugaccga ggggaugaga aagcccgcuu     1740

uucugucugg agaacagaag aaagccaucg ucgaucuucu cuucaagaca aaccgcaaag     1800

ugaccguuaa gcagcugaaa gaggacuauu ucaagaagau ugaauguuuu gauagcgugg     1860

agaucuccgg cgucgaggac cgguucaaug cuucucuugg cacauaccau gaccugcuca     1920

aaaucauuaa ggauaaagac uuucuggaua acgaagagaa ugaagacauc cuggaggaua     1980

uugugcuuac ccucacgcug uucgaagaca gagagaugau cgaggaacgg cugaagacau     2040

augcccaccu cuuugaugac aaagucauga agcagcugaa gcgcagacgg uacaccgggu     2100

ggggaagacu uagccgcaaa cugauuaacg gcauccggga uaagcaaucc gggaaaacca     2160

uccucgacuu ccuuaagagc gauggauuug cgaacagaaa uuucaugcag cugauucaug     2220

acgauucucu gacauuuaaa gaggacaucc agaaggccca gguguccggc caaggggaca     2280

gccuccacga acacauugcu aaccuggccg gcucucccgc uaucaagaaa ggcauucugc     2340

agaccguuaa gguggucgau gagcucguga aaguuauggg acgccauaag ccugaaaaua     2400

ucgugaucga gauggccaga gagaaccaga caacgcagaa agggcaaaag aacucccggg     2460

aacgcaugaa aagaauugag gaaggaauca aggagcuggg cagccagauu cuuaaggagc     2520

acccggucga aaauacccag cugcagaaug agaaacucua ccuguauuac cugcaaaacg     2580

ggcgggacau guauguggau caggaacuug acaucaaucg ccuguccgau uacgacgugg     2640

aucauauugu uccccagucu uuccucaagg acgauagcau cgacaacaaa guccugacaa     2700

gauccgauaa gaaucgggga aaaucugaca acgugccuag cgaggaagug gucaagaaga     2760

ugaaaaauua uuggcgccaa cuccugaacg cgaaacuuau cacccagaga aaauuugaca     2820

aucugacaaa ggccgagcgg ggcgggcucu ccgagcugga uaaagcuggc uucauuaagc     2880

gccagcuggu ggaaaccaga cagaucacga agcacguugc ccaaauucuu gacagccgga     2940

ugaacaccaa auacgaugag aacgacaagc ugaucagaga agugaaaguc auuacacuca     3000

agucuaaacu gguguccgau uuucgcaagg acuuccaguu cuauaagguu cgggagauca     3060

auaacuacca ccaugcucac gaugccuacc ucaaugccgu ggucggaacc gcucugauua     3120

aaaaguaucc caaacuugaa agcgaguuug uguacggcga cuauaagguu uacgauguga     3180

gaaaaaugau cgcgaagucu gagcaggaaa ucgggaaggc cacagcuaaa uauuucuuuu     3240

acuccaacau uaugaauuuc uuuaagaccg agaucacgcu ggccaacgga gaaauucgca     3300

aacggccucu caucgagaca aauggcgaaa ccggggagau ugucugggac aagggaagag     3360

auuucgccac agugcgcaaa gugcugagca ugccccaggu caacaucguu aagaaaaccg     3420

aggugcaaac cggcggguuu ucuaaggaaa gcauccugcc gaagagaaau uccgacaaac     3480

uuauugcucg gaagaaagau ugggacccua agaaauaugg cggauucgac agccccacag     3540

uggccuacuc uguccuggug guugcgaagg uggagaaggg caaauccaag aaacucaaga     3600

gcgucaaaga acugcucggg aucacgauua uggagcgcuc cucuuuugag aagaacccua     3660

ucgauuuccu ggaagcuaag ggauacaaag aggugaagaa agaccuuauu aucaagcugc     3720

ccaaauauag ccuguuugaa cucgagaaug gccggaagag aaugcuggcc uccgccgggg     3780

aacuucagaa gggaaacgag cuggcucucc cuucuaaaua cguuaauuuc cuguaucucg     3840

ccagccauua cgagaagcug aaaggcuccc ccgaagauaa cgagcagaag cagcuuuucg     3900

uggaacaaca caaacauuau cuggacgaga ucauugaaca gaucucugag uuuagcaagc     3960

gggucauucu ggcggaugcu aaucucgaca aagugcuguc cgccuacaac aagcaccgcg     4020

auaagccgau cagagagcag gccgaaaaca uuauccaccu uuucacccug acaaaucucg     4080

gggcuccugc cgcuuuuaaa uauuucgaca ccacaauuga ucggaagcgc uacaccagca     4140

cgaaagaggu ucuggacgcc acacucaucc aucaaucuau caccggccug uacgaaacca     4200

gaauugaucu uucccagcug ggaggcgaca agcggcccgc ggccacaaag aaggcugggc     4260

aggccaagaa aaagaaauaa ggacuagugu ccaccugucc cuccugggcu gcuggauugu     4320

cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa aaaaaaaaaa     4380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4433


<210>  4843
<211>  5216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4843
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa cuucaagauu cugccgaucg cgauugaccu cggaguuaaa aauacaggcg      180

uguuuagcgc uuucuaccag aaggggacau cccuggagcg gcuugauaac aagaauggaa      240

aaguguauga acugucuaag gacagcuaca cgcuccugau gaacaaucgc accgccagac      300

ggcaucaacg cagaggcauc gaucggaaac agcuugucaa gcgccuguuu aaacucauuu      360

ggacagagca gcugaaccug gaaugggaca aggauaccca gcaagccauc uccuuccucu      420

uuaauagacg gggguucucu uuuaucacag acggauauag ccccgaguac cugaacauug      480

ugccugaaca gguuaaggcu auccuuaugg auauuuucga cgauuauaau ggcgaggacg      540

aucuggacuc cuaccucaaa cuggccaccg agcaggaauc uaagaucagc gagauuuaua      600

acaaacuuau gcagaagauc cuggaauuua aacucaugaa gcugugcaca gauauuaaag      660

acgacaaggu guccaccaag acgcugaaag agaucaccag cuacgaguuc gaacuccugg      720

cugauuaccu ugccaauuau ucugaguccc ugaagacaca aaaauuuagc uacaccgaca      780

agcagggcaa ccucaaagaa cugucuuauu accaccauga uaaguauaau auccaggagu      840

uccuuaagcg ccacgcgaca auuaacgaca gaauccugga uacccuccug acggacgauc      900

uggacauuug gaauuuuaac uucgaaaaau ucgauuuuga caagaacgag gagaaacucc      960

agaaucaaga agauaaggac cauauccagg cccaucugca ccacuucguc uuugcuguga     1020

acaaaauuaa guccgagaug gccagcgggg gacggcaccg cucucaguac uuccaagaaa     1080

ucacaaaugu ucuugacgag aacaaucauc aggaaggcua ucugaagaac uuuugugaga     1140

aucuccacaa caaaaaguac uccaaucuga gcgugaaaaa ccuugucaau cugaucggga     1200

accucuccaa ucuggagcug aagccccuca gaaaauacuu caacgauaag auucacgcua     1260

aggccgacca uugggaugaa cagaaauuua ccgagacaua uugccacugg auccugggag     1320

aauggcgggu gggcgugaag gaccaggaua aaaaggacgg ggcgaaauac ucuuauaagg     1380

aucuuuguaa ugagcugaaa caaaagguca ccaaggccgg acucguugac uuccugcugg     1440

aacuugaucc uugccgcacc auucccccuu accucgacaa caacaauaga aaaccgccca     1500

agugucagag ccugauccug aacccuaaau uucucgauaa ucaguauccc aacuggcagc     1560

aauaccugca ggagcuuaag aaacugcagu ccauucagaa uuaucucgac agcuucgaga     1620

cagaucugaa ggugcugaag ucuuccaaag accaaccuua cuuuguggaa uacaagagcu     1680

cuaaccagca gaucgcuagc ggccagcggg acuauaaaga ucuugacgcc agaauccucc     1740

aauucauuuu ugaucgcguc aaggcuuccg acgagcugcu gcucaaugaa aucuacuucc     1800

aggccaaaaa gcugaagcag aaagccucua gcgagcuuga gaagcuggaa uccagcaaaa     1860

agcucgauga ggugauugcu aacucucaac ugucccagau ccugaaaagc cagcauacga     1920

auggcauuuu cgaacagggg accuuucuuc accucguuug caaguauuac aagcaacggc     1980

agagagcgcg cgacucucgg cuguauauca ugcccgagua cagauaugau aaaaagcugc     2040

auaaauacaa caauacagga cgcuucgacg augacaacca gcuccugacc uacuguaauc     2100

acaagccgcg gcagaaaaga uaucaacuuc ugaacgaucu cgccggcgug cugcaggucu     2160

ccccuaauuu ucugaaggac aaaauuggga gcgaugacga ccuuuucauc uccaaguggc     2220

ucguggaaca cauccgcgga uuuaagaagg cuugcgagga uucucugaaa auucagaaag     2280

acaaccgggg ccugcucaac cauaagauca auauugccag aaacacaaaa gggaagugug     2340

agaaggaaau cuucaaucug auuugcaaaa ucgagggaag cgaagauaag aaaggcaacu     2400

acaagcacgg ccuugccuau gagcuggggg uucuccuguu uggagaaccc aaugaggcuu     2460

ccaaaccuga guucgaccgc aagaucaaga aauuuaacuc uauuuacagc uucgcccaga     2520

uccaacagau ugcguuugcu gaacggaagg gcaaugccaa caccugugcc gugugcuccg     2580

cugauaaugc ccauagaaug cagcaaauca aaauuacgga gcccgucgaa gacaacaagg     2640

auaaaaucau ccugagcgcu aaggcccagc ggcuucccgc gauuccuaca cgcaucgugg     2700

acggggccgu caagaaaaug gcuaccauuc uggccaagaa uaucguggau gacaacuggc     2760

agaauauuaa acagguucuc ucugcuaagc accaacugca caucccgauc auuaccgagu     2820

ccaacgccuu cgaauuugag cccgcgcucg ccgaugugaa aggaaagagc cugaaggaca     2880

gacggaaaaa ggcucuugag agaaucucuc cugaaaauau uuucaaagac aagaacaacc     2940

gcaucaaaga guucgccaag ggcauuuccg cuuauagcgg ggccaaucug acagauggcg     3000

acuuugaugg agccaaagag gaacucgacc auaucauucc ccggucucac aagaaguacg     3060

gcacccugaa cgaugaggcu aaucugaucu gcgucacaag aggggacaac aaaaauaagg     3120

gaaaccgcau cuucugucuu agagaucugg cggacaauua uaaacucaag caguuugaaa     3180

cgaccgauga ccuggagauu gagaaaaaga ucgccgauac aauuugggac gcuaacaaga     3240

aagauuucaa guuuggcaau uaccggagcu ucaucaaccu caccccucag gaacagaaag     3300

ccuuucgcca ugcccuguuc cuugcugacg aaaaucccau uaagcaagcc gugaucagag     3360

cgaucaacaa ucggaaccgc acauuuguua augggaccca gagauacuuc gcugaggugc     3420

uggccaacaa uauuuaucuc cgggccaaaa aggagaaccu gaacacggac aagaucuccu     3480

uugauuacuu cggcauuccg accaucggaa augggcgcgg cauugcugaa aucagacagc     3540

uguaugagaa agucgacagc gauauccagg ccuacgcgaa gggagacaaa ccucaggcuu     3600

cuuauuccca ccuuauugau gccaugcugg ccuucugcau cgcugccgac gaacaucgga     3660

acgauggcag cauugggcuc gagaucgaca agaauuacuc ccuguauccc cucgauaaaa     3720

acacaggaga aguguuuacc aaggacauuu ucucucaaau caagauuaca gauaaugagu     3780

uuagcgacaa aaagcuggug cgcaaaaagg cuaucgaggg cuucaacacc cacagacaga     3840

ugacgcggga ugggaucuac gccgaaaauu accuuccuau ucugauccac aaagagcuga     3900

acgaaguuag aaagggauau acauggaaaa auuccgagga aauuaagauc uuuaagggca     3960

aaaaguacga cauucagcaa cucaacaauc uggucuauug ucuuaaauuc guggacaagc     4020

ccaucucuau cgauauucag aucagcaccc uggaggagcu ccgcaacauu cugacaacca     4080

auaacaucgc ggccaccgcu gaauacuauu acauuaaucu caaaacacag aagcugcaug     4140

aguauuacau cgaaaacuac aacacggccc uuggguauaa gaaauacucc aaggagaugg     4200

aguuucugcg gucucuggcu uauagaagcg aacgcgugaa aaucaagucc auugacgaug     4260

ucaaacaagu gcucgacaag gauagcaauu ucaucauugg caagaucacc cugccuuuua     4320

aaaaggagug gcagcggcuu uacagagaau ggcagaacac aaccauuaaa gacgauuaug     4380

aguuccugaa gucuuucuuc aauguuaaau ccaucacaaa gcuccacaag aaagugcgca     4440

aggacuuuag ccugcccauu ucuaccaacg aaggaaaauu ccucgucaag cggaaaacgu     4500

gggauaauaa cuucaucuac cagauccuga augacuccga uagcagagcc gacggcacaa     4560

agccguuuau uccugccuuc gacaucucua aaaacgagau uguggaggcu aucauugauu     4620

ccuuuaccag caagaauauc uucuggcuuc ccaagaacau cgaacugcaa aaaguugaca     4680

auaagaacau uuuugcgauc gauaccucca aaugguucga gguggaaaca cccagcgacc     4740

ugcgcgauau ugggaucgcc accauucagu auaagaucga caauaacucu cggccuaaag     4800

ucagagugaa gcucgauuac guuaucgacg augacagcaa gauuaauuac uuuaugaacc     4860

auucccugcu uaaaucucgg uauccggaua aggugcugga gauccucaaa cagagcacaa     4920

uuaucgaauu ugaguccucu ggauuuaaca agacgauuaa agagaugcug ggcaugaagc     4980

ucgcugggau cuacaaugaa accagcaaca auaagcgccc cgccgccacc aaaaaggcug     5040

gacaggccaa aaagaaaaag uaaggacuag uguccaccug ucccuccugg gcugcuggau     5100

ugucucguuu uccugccaaa uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa     5160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         5216


<210>  4844
<211>  3488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4844
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gcggaacuac auucugggac ucgacaucgg cauuaccagc guuggguaug      180

gaaucauuga uuacgagaca cgcgacguga ucgaugcggg cgugagacug uucaaagaag      240

cuaaugucga gaacaaugaa gggcggcgcu ccaagagagg agcccggcgc cuuaagagac      300

ggcgcagaca ucggauccag cgcgugaaaa agcugcucuu ugacuauaac cugcuuacgg      360

aucacucuga gcugagcggc auuaauccgu acgaagccag aguuaaaggc cucucccaaa      420

agcugucuga ggaggaguuc agcgcugccc ugcuccaucu ggcuaaacgg cgcggggugc      480

acaacgucaa ugaaguggaa gaggacaccg gaaacgagcu uuccacaaag gaacagaucu      540

cuagaaauag caaggcccug gaggaaaaau auguugcgga gcuccagcug gaacggcuua      600

agaaagaugg cgaggugaga ggguccauua accgcuuuaa gaccagcgac uacgucaaag      660

aggccaagca gcugcucaaa gugcaaaagg cuuaucauca gcuggaucag ucuuucaucg      720

acacauacau ugaucugcuc gaaacccgga gaacauacua ugagggaccc ggcgaagggu      780

ccccuuuugg auggaaggac aucaaagagu gguacgaaau gcugaugggc cacugcaccu      840

auuuccccga ggagcuucgc agcgugaagu acgccuauaa ugcugaucug uacaacgccc      900

ucaaugaccu gaacaaucuu gucauuacgc gggacgaaaa cgagaaacug gaauauuacg      960

agaaguuuca gaucaucgaa aacguuuuca aacaaaagaa gaaaccuacc cucaagcaga     1020

uugcgaaaga gauccuggug aaugaggaag auauuaaggg cuacagagug acaucuaccg     1080

ggaaacccga guuuacaaac cugaaggucu aucacgacau caaggauauu accgcccgca     1140

aagaaaucau cgagaaugcu gagcuccugg accagauugc caagauccuu acgauuuacc     1200

aguccagcga agauauccaa gaggaacuga caaaccucaa uucugagcug acccaggaag     1260

agauugagca gaucuccaac cuuaaaggau auacaggcac ccauaaucug agccucaagg     1320

cuaucaaccu gauucuggac gaacucuggc acaccaauga uaaccaaauc gccauuuuca     1380

aucggcugaa acuugugccu aagaagguug accuguccca gcagaaagag aucccgacaa     1440

cgcucgugga ugacuuuauu cugucucccg ucgugaagcg cagcuucauc caguccauua     1500

aaguuaucaa cgccaucauu aagaaauacg ggcugccuaa ugauaucauu aucgaacuug     1560

cucgcgagaa gaacagcaaa gacgcgcaaa agaugauuaa ugaaaugcag aagcggaaca     1620

gacagaccaa cgagcgcauc gaggaaauca uucggacaac cggaaaagag aaugccaagu     1680

aucucaucga gaaaauuaag cugcaugaca ugcaggaagg caaaugucug uacucucucg     1740

aagcuauccc ccuggaggau cuucugaaca auccuuuuaa cuaugaggug gaccacauua     1800

uccccagauc cgucagcuuc gauaauucuu ucaacaauaa ggugcucguc aagcaagaag     1860

agaacagcaa aaaggggaau cggacaccgu uucaguaccu guccucuagc gacuccaaaa     1920

ucagcuacga aaccuucaag aaacacauuc ugaaccuugc caagggaaag ggccgcaucu     1980

cuaaaacgaa gaaagaguau cuccuggagg aaagagauau uaaucgguuu uccgugcaga     2040

aggacuucau caacagaaau cugguugaua cacgcuacgc cacccggggc cucaugaacc     2100

ugcuuagaag cuauuuucgc gugaauaacc uggacgucaa agugaagucu auuaacgggg     2160

gauucaccuc cuuucucaga cggaagugga aauucaagaa agagcgcaau aagggcuaca     2220

aacaucacgc ugaagaugcc cugaucaucg cgaacgcuga cuuuauuuuc aaggagugga     2280

aaaagcugga uaaggccaaa aagguuaugg aaaaucagau guuugaggag aaacaagccg     2340

aaagcaugcc ugagaucgaa acagagcagg aauauaagga gauuuucauc acaccccauc     2400

agauuaaaca caucaaggac uuuaaggauu acaaauauuc ccauagagug gacaagaaac     2460

cuaaccggga gcuuauuaau gacacccucu acucuacgcg caaggaugac aaagggaaca     2520

cccugaucgu caauaaccug aauggacucu acgauaagga caacgauaag cugaagaaac     2580

uuaucaauaa aagccccgaa aagcugcuca uguaucacca cgacccccag acauaccaaa     2640

aacugaagcu gauuauggag caguauggcg augaaaagaa cccucuuuac aaauauuacg     2700

aggaaaccgg gaauuaucuc acaaaguacu ccaaaaagga caacggcccg gugaucaaaa     2760

agauuaagua cuauggaaau aaacugaacg cucaucugga uaucaccgac gauuacccca     2820

acucuagaaa uaaggugguu aaacucagcc ugaagccuua ucgguucgac gucuaccuug     2880

auaacggcgu guauaaauuc gugacgguca agaaucugga cgugauuaaa aaggagaacu     2940

acuaugaggu uaauuccaag ugcuacgaag aggccaaaaa gcucaaaaag aucagcaacc     3000

aggcugaauu uaucgccucu uucuacaaua acgaccugau uaaaaucaau ggggagcugu     3060

aucgcgugau uggagucaac aaugaucuuc ugaacagaau cgaggugaau augauugaca     3120

ucaccuaccg ggaauaucuc gagaacauga augauaagag acccccuaga aucauuaaga     3180

caaucgcguc caaaacccaa agcauuaaga aauacucuac agacauccug ggcaaccucu     3240

augaaguuaa guccaaaaag cacccccaga uuaucaagaa agggaagcgg ccggccgcua     3300

ccaaaaaggc cggacaggcu aaaaagaaga aauaaggacu aguguccacc ugucccuccu     3360

gggcugcugg auugucucgu uuuccugcca aauaaacagg aucagcgcuu uacagaucua     3420

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaagaauu                                                              3488


<210>  4845
<211>  4556
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4845
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugcccu guucaacaag ugcauuauca uuagcaucaa ucucgacuuu uccaacaaag      180

agaaguguau gaccaagccg uacucuauug gacuggauau cggcacaaau agcguugggu      240

gggcggugau cacggacaac uauaaagugc ccuccaagaa aaugaagguc cuuggaaaua      300

ccucuaaaaa guacauuaag aaaaaccugc ucggcgugcu gcuuuucgau agcgggauca      360

cagcugaagg acggcgccug aagagaaccg cccggcgcag auauacacgg cgcagaaauc      420

ggauucucua ccugcaggag aucuuuucca ccgaaauggc cacacuggac gaugcuuucu      480

uucaacgccu cgacgauucu uuccugguuc cugacgauaa aagagacagc aaguauccca      540

uuuuuggcaa ccuuguggag gaaaaagucu accaugauga guucccuacc aucuaucauc      600

ugcggaagua ccucgccgac uccacgaaaa aggcugaucu gcgccuugug uaccuggccc      660

ucgcgcacau gauuaaguau agaggccacu uucugaucga gggggaguuc aauagcaaaa      720

acaaugacau ccagaagaac uuucaggacu uccuggauac cuacaaugcc auuuuugaau      780

cugaccucuc ccuggaaaac agcaaacagc uugaggagau cguuaaggau aaaauuucua      840

agcuggaaaa gaaagaccgg auccucaagc uguuccccgg agagaaaaau uccggcauuu      900

ucagcgaauu ucuuaagcug aucgugggga accaagcuga uuuccgcaaa ugcuuuaacc      960

ucgacgagaa ggccucucug cauuucucca aggaaagcua ugaugaggac cuggagacac     1020

uccugggaua caucggcgau gacuauuccg augucuuucu uaaagcuaag aaacuguacg     1080

acgccauucu ccugucuggg uuccuuaccg ugacagacaa ugaaaccgag gcgccucuga     1140

gcuccgccau gaucaagaga uauaacgaac acaaagagga ucucgcucug cugaaggaau     1200

acauucggaa uaucagccuc aagacguaca acgagguguu uaaagacgau acaaagaaug     1260

gauaugccgg cuacauugac ggcaaaacca accaggagga uuucuauguc uaccugaaga     1320

aucuucuggc ugaauuugag ggggccgacu auuuccucga aaagaucgau agagaggacu     1380

uucugcgcaa gcagcggaca uucgauaacg gaucuauccc guaccagauu caucugcaag     1440

aaaugagagc cauccuugac aaacaggcua aguuuuaucc cuuccucgcg aagaauaaag     1500

agcgcauuga gaagauccug accuuccgga uuccuuacua cguuggcccc cuggccagag     1560

ggaacuccga uuuugcuugg agcauccgca aacggaauga aaagauuacc ccuuggaacu     1620

ucgaggacgu gaucgauaaa gaaucuagcg ccgaggccuu uaucaauaga augacauccu     1680

ucgaccucua ucugcccgag gaaaaggugc uuccgaagca cucucugcuc uacgagacgu     1740

uuaacgucua uaaugaacug accaaagugc gcuucauugc ugagagcaug cgggacuacc     1800

aguuucugga uuccaagcag aaaaaggaca ucguuagacu uuauuucaaa gauaagcgca     1860

aggugacaga caaagauauu aucgaauacc uccacgccau uuauggauac gacggcaucg     1920

agcugaaggg gaucgagaaa caauuuaaca gcucucuguc caccuaccau gaucuccuga     1980

acauuaucaa ugacaaggag uuccuugaug acagcucuaa cgaagcgauu aucgaagaga     2040

uuauccacac acugaccauc uuugaagauc gggagaugau uaaacagaga cucuccaagu     2100

ucgagaauau cuuugacaag agcguccuga aaaagcuguc ccggcgccac uauacgggau     2160

ggggcaaacu uucugcuaag cucauuaacg gcaucagaga ugaaaagagc gggaauacaa     2220

uucuggacua ccugaucgac gauggaaucu ccaaccggaa uuucaugcag cucauucaug     2280

acgaugcccu gucuuucaag aaaaagaucc aaaaggccca gauuaucggc gacgaggaua     2340

aagggaacau uaaggaagug guuaaaagcc uuccuggauc ccccgcuauc aagaaaggca     2400

uucugcagag caucaagauc guggacgagc ucgucaaggu gaugggcggg agaaaaccug     2460

aaucuauugu cguggagaug gcccgcgaga aucaguauac caaccaagga aaguccaaua     2520

gccagcagcg gcugaaaaga cuggaaaagu cucuuaaaga gcucggcucc aagauccuga     2580

aggaaaacau ucccgcuaaa cugagcaaga ucgauaauaa cgcccuccag aaugaccgcc     2640

uguaccuuua uuaccugcaa aacgggaaag auauguauac cggagacgau cucgacauug     2700

acagacuguc uaacuacgau aucgaccaca ucauucccca ggcguuucug aaggauaaua     2760

gcaucgacaa caaaguucuu guguccagcg ccucuaaucg gggcaagucc gaugacuucc     2820

cuagccugga ggucgugaag aaacgcaaga cauuuuggua ccagcuccug aaauccaagc     2880

ucauuucuca gagaaaauuc gauaaccuga ccaaggcuga gcgcggggga cuucugccgg     2940

aagacaaggc cggcuuuauc caacggcagc ucguugagac aagacagauu acgaaacaug     3000

uggcucggcu gcuggaugaa aaguucaaua acaaaaagga cgagaauaac cgcgccguca     3060

gaaccgugaa aaucaucaca cuuaagagca cccugguguc ccaguuucgg aaagauuucg     3120

aacucuauaa gguucgcgag auuaaugacu uucaccaugc gcacgaugcc uaccugaacg     3180

cugucaucgc cucugcucuc cugaagaaau aucccaaacu ugagccugag uucguguacg     3240

gggacuaucc caaauacaau agcuuuagag aacggaaguc cgccacagaa aaaguguauu     3300

ucuacucuaa cauuaugaau aucuucaaaa agagcauuuc ccuggccgac ggcagaguca     3360

ucgagcgccc ucucauugaa gugaacgagg agaccggaga aagcguuugg aauaaagagu     3420

cugaucuggc uacggugcgg agaguccugu ccuaccccca agugaacguu gugaagaaag     3480

ucgaggaaca gaaccacggc cuugaccgcg ggaagccgaa aggacuguuu aaugcgaacc     3540

ucagcucuaa gccuaagccc aauuccaacg aaaaucuggu gggcgccaaa gaguaucucg     3600

auccuaagaa auacggggga uaugcuggca ucagcaacuc uuucgccguu cuggugaagg     3660

ggaccaucga gaaaggcgcc aagaagaaaa uuacaaaugu ccuugaauuu cagggaaucu     3720

ccauucugga ccggaucaac uacagaaagg auaaacugaa uuuccuccug gagaagggcu     3780

auaaagacau ugaacuuauc aucgagcugc ccaaguacag ccucuuugag cuguccgaug     3840

ggagccgccg gaugcucgcu ucuauucuga gcaccaacaa uaaaagagga gaaauccaua     3900

agggcaacca aauuuuccuu ucccagaagu uugugaaacu gcuguaucac gccaagcgca     3960

ucucuaauac aauuaacgag aaucaucgga aauacgugga aaaccacaag aaagaguucg     4020

aggaacucuu uuacuauauc cuggaguuca augaaaacua cgucggggcg aagaagaacg     4080

gaaaacuucu gaauagcgcu uuucaguccu ggcagaacca cucuaucgac gagcucugua     4140

gcuccuucau uggcccuacc gggucugaaa gaaagggccu guuugagcuc acgagccggg     4200

gauccgccgc cgauuucgag uuccugggcg uuaaaauccc ccgcuauaga gacuacacac     4260

cgagcucucu ucugaaggau gcuacccuga uucaucaauc cgugacaggg cucuaugaaa     4320

cccggaucga ccuggccaaa cuuggagagg gcaagcgccc ugcggcuacc aagaaagccg     4380

ggcaggccaa gaaaaagaaa uaaggacuag uguccaccug ucccuccugg gcugcuggau     4440

ugucucguuu uccugccaaa uaaacaggau cagcgcuuua cagaucuaaa aaaaaaaaaa     4500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agaauu         4556


<210>  4846
<211>  3632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4846
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccug guacgcgagc cugauguccg cucaucaccu ccggguugga auugacgugg      180

gcacccauuc uguggggcug gccacacuuc gcgucgauga ccacggaacg ccgaucgagc      240

ugcucagcgc ccugucccau auucacgauu cuggcguggg gaaggaaggg aaaaaggacc      300

acgauaccag aaagaaacuu agcggcaucg cucggcgcgc cagacggcug cuccaucacc      360

gcagaacaca gcugcaacag cuggacgagg uucuccggga ucugggcuuc cccauuccua      420

cccccgggga auuucuugac cugaacgagc agacagaucc uuaucgcgug uggagagucc      480

gggcucgccu cguggaagag aagcugcccg aggaacuuag aggaccugcc aucuccaugg      540

cgguucggca uaucgcccgc cacagaggcu ggcggaaucc guacucuaaa guggagagcc      600

ugcucucccc cgcugaagag agcccuuuca ugaaggcccu gagagagcgc auucuggcua      660

ccacagggga aguccucgac gauggaauca cccccggcca ggccauggcg caaguggccc      720

ugacgcacaa cauuucuaug cgggggccug agggaauccu uggcaaacug caucaguccg      780

acaaugcuaa cgaaauuaga aagaucugcg cccgccaggg cgugagcccc gaugucugua      840

agcagcuccu gcgggcuguu uuuaaagccg acucuccgag aggguccgcc gugagccgcg      900

uggcuccuga cccccuuccu ggacaaggcu cuuuccggag agcgcccaag ugcgaucccg      960

aguuucagcg cuuccggauu aucuccaucg ucgccaaucu gagaauuagc gaaaccaaag     1020

gggagaaccg cccucucaca gcugacgagc ggagacacgu gguuaccuuu cugacagaag     1080

auucccaggc cgaccugacc uggguggaug ucgccgagaa gcucggagug caucggcgcg     1140

accugagagg cacggcuguu cacacagaug acggggaacg gucugccgcg agaccgccca     1200

ucgaugcuac cgaccgcauu augcggcaga caaaaaucag cucccuuaag accugguggg     1260

aggaagccga uagcgagcaa agaggagcca ugauucgcua ucuguacgag gacccuaccg     1320

acucugaaug ugcugagauc aucgccgaac uccccgagga agaucaggcu aaacuggacu     1380

cccuucaucu gccugccggc agagcggccu auagccggga gucucucaca gcucugagcg     1440

aucacaugcu ggccacgacc gacgaucucc acgaggcucg caagagacug uucggcgugg     1500

acgauuccug ggcgcccccg gccgaagcca uuaaugcucc ugucgggaac cccucugugg     1560

accggacacu uaagaucguc ggacgcuacc ugagcgccgu ggaguccaug uggggcaccc     1620

cugaaguuau ucauguggag cacgucagag auggguuuac aagcgagcgg auggcugacg     1680

aacgcgauaa agccaauaga cggcgcuaua acgacaauca ggaggccaug aagaaaaucc     1740

aaagagauua cggaaaggaa ggcuacauuu cucggggaga caucgugaga cucgacgcuc     1800

uggagcugca gggcugcgcg ugucuuuauu gcggaaccac gaucggcuac cauacauguc     1860

agcucgauca cauuguuccc caggccgggc cuggauccaa caaucgccgg ggcaaccugg     1920

uggcugucug cgaaagaugu aaucgcagca aaucuaacac ccccuucgcc gugugggccc     1980

aaaagugcgg gaucccgcac gugggaguua aggaggcuau uggccggguc agaggguggc     2040

gcaaacagac cccuaacaca uccagcgagg accugacccg gcucaagaaa gaagugaucg     2100

ccagacugcg ccggacacag gaggaucccg aaauugacga gagauccaug gaaucugugg     2160

cguggauggc uaaugagcuu caucaccgga ucgccgccgc uuaucccgag acgaccguca     2220

ugguguaccg cggcagcauu acagccgcgg cuagaaaggc cgccggaauc gauucccgga     2280

ucaaccugau uggcgaaaaa gggcgcaagg acagaaucga ucggagacau cacgcuguug     2340

acgccucugu ggucgcucuc auggaggcca gcguggcgaa gacccuggcc gaacgcucca     2400

gccugcgggg agagcagaga cuuacaggca aagaacaaac cuggaagcag uauacggggu     2460

cuaccguugg agcucgcgag cauuuugaga uguggcgggg ccacaugcuc caccugacag     2520

aacuguucaa ugagagacuc gccgaagaua aaguguacgu cacccagaac auucgccugc     2580

ggcuuuccga cgggaaugcu cauacaguga acccuagcaa gcugguuucu cacagacucg     2640

gcgauggacu gaccgugcag caaaucgacc gcgccuguac gccggcccug uggugcgcuc     2700

uuacaagaga gaaagauuuu gacgagaaga auggccuccc cgcgcgggaa gaccgcgcca     2760

uuagagucca ugggcacgag aucaagucca gcgauuauau ccagguguuc ucuaaacgga     2820

agaaaaccga cagcgaucgc gacgaaacac cuuucggagc uauugccgug agaggcgggu     2880

uugucgagau cggacccucc auucaucacg cccggaucua cagaguugaa ggcaagaaac     2940

cuguguacgc uaugcugcgc guguucaccc acgaucugcu cagccagcgg cauggggacc     3000

uguuuucugc cgucauuccc ccucagucca ucagcaugag augcgcggag cccaaacuuc     3060

gcaaagcuau caccacaggc aacgccacgu aucugggaug ggugguugug ggcgaugagc     3120

ucgaaauuaa ugucgacucc uucaccaagu acgccaucgg gcgguuucug gaggauuucc     3180

cgaacacaac cagauggcgg auuuguggau augacacaaa uucuaagcug acccuuaaac     3240

cuaucgugcu cgcugccgaa ggccuggaga accccagcuc cgcgguuaau gaaauugugg     3300

agcugaaggg guggcgcguc gcuaucaacg ugcucacgaa aguucacccu acagugguca     3360

gacgggaugc ccugggacgc cccagauacu cuagccgguc caaucuuccg accucuugga     3420

ccaucgagaa gcgcccugcc gcuacaaaga aggccggcca agcuaagaaa aagaaauaag     3480

gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu gccaaauaaa     3540

caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3600

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3632


<210>  4847
<211>  4493
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4847
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccac caagccguac agcauuggac uggacaucgg cacaaacucc guuggguggg      180

cggugacgac cgauaauuau aaagugcccu cuaagaagau gaaaguccuc ggaaacacaa      240

gcaagaaaua cauuaagaaa aaucugcuug gcgugcuccu guucgacucc gggaucaccg      300

cugagggacg gcgccugaag agaacagccc ggcgcagaua uacccggcgc agaaaccgga      360

uucuuuaccu gcaggaaauc uuuucuacag agauggccac ccucgaugac gcuuucuuuc      420

aacgccugga ugacagcuuc cugguuccug augacaagag agauuccaaa uaucccaucu      480

uuggcaaucu cguggaagag aaggccuacc augacgaguu cccuacgauu uaucaucugc      540

ggaaauaccu ugcugauucu accaagaaag ccgaccugcg ccucgucuac cuggcgcuug      600

cccacaugau caaguauaga ggccacuuuc ugauugaggg ggaguucaac agcaaaaaua      660

acgauaucca gaagaauuuu caggacuucc ucgacacaua caacgcuauu uuugaauccg      720

aucugagccu ggaaaauucu aagcagcucg aagagaucgu gaaagacaag auuuccaaac      780

uggagaagaa agaucggauc cuuaagcugu uccccggaga aaagaacagc ggcaucuuuu      840

cugaguuccu caaacugauu guugggaauc aagccgacuu ccgcaagugc uuuaaccuug      900

augaaaaggc uucccugcau uucagcaaag agucuuauga cgaagaucuc gagacccugc      960

ugggauacau cggcgacgau uauuccgacg uguuucucaa agccaagaag cuguacgaug     1020

cgauucuucu gagcggguuc cucacaguca ccgacaauga gacggaagcc ccucuguccu     1080

cugcuaugau caaaagauau aacgagcaca aggaagaccu ugcccugcuc aaagaguaca     1140

uucggaacau cagccugaag acauacaaug aaguguuuaa agaugacacc aagaacggau     1200

augcuggcua caucgauggc aagacaaauc aggaggacuu cuauguguac cugaaaaagc     1260

uccuggccga guuugaaggg gccgauuauu uccuugagaa aauugacaga gaagauuuuc     1320

ugcgcaagca gcggaccuuc gacaacggau ccaucccgua ccagauucac cuccaagaga     1380

ugagagcuau ccuggauaaa caggcgaagu uuuaucccuu ccuggccaaa aauaaggaac     1440

gcauugagaa gauccuuacc uuucggaucc cuuacuacgu cggcccccuc gcuagaggga     1500

acagcgacuu cgccuggucu auucgcaaac ggaaugagaa gaucacaccu uggaacuucg     1560

aagauguuau ugacaaagag uccagcgccg aggcuuuuau caauagaaug acgucuuucg     1620

aucuguaucu gcccgaagag aaggugcucc cgaaacauag ccugcuuuac gaaaccuuua     1680

acguguauaa ugagcugaca aagguccgcu ucauugccga auccaugcgg gacuaccagu     1740

uucucgacuc uaagcagaaa aaggauaucg ugagacugua uuucaaagac aagcgcaaag     1800

uuaccgauaa ggacauuauc gaguaccugc acgcgaucua uggauacgau ggcauugaac     1860

uuaaggggau cgagaaacaa uuuaacagcu cccucagcac auaccaugac cugcugaaua     1920

uuaucaacga uaaggaguuc cucgacgauu cuuccaauga agcuauuauc gaggaaauca     1980

uucacacccu gacgaucuuu gaggaccggg aaaugauuaa acagagacuu agcaaguucg     2040

agaacaucuu ugauaaaucu gugcugaaga agcucucccg gcgccacuau acaggauggg     2100

gcaaacugag cgccaagcug auuaacggca ucagagacga gaaauccggg aauaccaucc     2160

uugauuaccu cauugacgac ggaaucucua accggaauuu caugcagcug auucaugaug     2220

acgcccugag cuuuaagaaa aagauccaaa aagcucagau uaucggcgau gaagacaagg     2280

ggaacaucaa ggaggucgug aaaucccucc cuggaucucc cgccauuaag aaaggcaucc     2340

ugcagagcau uaagaucguu gaugaacuug ugaaagucau gggcgggaga aagccugagu     2400

ccauuguggu cgaaauggcu cgcgagaauc aguauaccaa ccaaggaaag agcaauucuc     2460

agcagcggcu gaaaagacuc gagaaguccc ugaaagaacu gggcagcaag auccuuaaag     2520

agaacauucc cgccaagcuc ucuaagaucg acaauaacgc gcugcagaau gaucgccugu     2580

accucuauua ccugcaaaac gggaaagaca uguauacagg agaugaccuu gauaucgaca     2640

gacuguccaa uuacgacauu gaucacauca uuccccaggc cuuccucaag gacaacagca     2700

ucgauaauaa agugcugguu ucuagcgcuu ccaaccgggg caagagcgac gaugugccuu     2760

cucuggaagu cgugaaaaag cgcaagaccu ucugguacca gcuucugaaa uccaagcuca     2820

uuagccagag aaaauuugac aaccugacaa aggccgagcg cgggggacuc uccccggagg     2880

auaaagcugg cuucauccaa cggcagcugg uugaaacgag acagaucacc aagcaugugg     2940

cccggcuucu ggacgagaag uuuaauaaca aaaaggauga aaauaaccgc gcggucagaa     3000

cagugaaaau uaucacccuc aagucuacac uggugagcca guuccggaaa gacuuugagc     3060

uguauaaggu ucgcgaaauu aaugauuucc accaugccca cgacgcuuac cuuaacgccg     3120

ucguggcuuc cgcccugcuc aaaaaguauc ccaagcugga gccugaguuu guguacgggg     3180

auuaucccaa auacaauucu uucagagaaa gaaagagcgc caccgagaaa gucuauuuuu     3240

acuccaacau caugaauauu uucaagaaau cuaucagccu cgcugacggc cgggugaucg     3300

aacgcccucu gauugagguu aacgaagaga cgggagaguc cguguggaau aaggaaagcg     3360

accuugcgac cguccggaga gugcugucuu acccccaagu uaacgugguc aagaaagugg     3420

aggaacagaa ucacggccuc gaucgcggga agccgaaagg acuguuuaac gccaaucugu     3480

ccagcaagcc uaaacccaac ucuaacgaga aucuuguugg cgcuaaggaa uaucuggacc     3540

cuaagaaaua cgggggauau gccggcaucu ccaacagcuu cacagugcuc gucaagggga     3600

ccauugagaa aggcgccaag aaaaagauca caaaugugcu ggaguuccag ggaauuucua     3660

uccucgaucg gauuaacuac agaaaggaca aacugaauuu ucuucuggaa aagggcuaua     3720

aagauaucga gcugaucauu gaacucccca aguacucccu guucgagcuu agcgacgggu     3780

cccgccggau gcuggcuagc auccucucua ccaacaauaa aagaggagag auucauaagg     3840

gcaaccaaau cuuucugagc cagaaauucg ugaagcuccu guaucacgcc aagcgcauuu     3900

ccaauacgau caacgaaaau caucggaaau acgucgagaa ccacaagaaa gaauuugagg     3960

aacuuuucua cuauauccug gaguuuaaug agaacuacgu uggggcgaag aaaaauggaa     4020

agcugcucaa cucugcuuuc cagagcuggc agaaucacuc cauugaugaa cuguguucua     4080

gcuuuaucgg cccuacaggg uccgagagaa agggccuuuu cgaacugacc ucucggggaa     4140

gcgccgccga cuuugaguuc cucggcguga aaauuccccg cuauagagau uacacaccgu     4200

ccagccugcu caaggacgcu acccugaucc aucaaucugu gaccgggcuu uaugaaacac     4260

ggauugaucu ggccaaacug ggagagggca agcgcccugc ggcuacgaaa aaggccgggc     4320

aggccaagaa aaagaaauaa ggacuagugu ccaccugucc cuccugggcu gcuggauugu     4380

cucguuuucc ugccaaauaa acaggaucag cgcuuuacag aucuaaaaaa aaaaaaaaaa     4440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaga auu            4493


<210>  4848
<211>  3575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4848
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc ggcuuucaag ccgaacagca uuaauuacau ccugggacuc gacauuggca      180

ucgccuccgu ugggugggcc augguggaga uugaugaaga ggaaaacccc auccggcuga      240

ucgaccuugg agugcgcguc uuugagagag cugaagugcc uaaaaccggc gauucucugg      300

ccauggcucg gcgccucgcc agaagcguuc ggcgccugac aagacggcgc gcgcauagac      360

uucugcggac gcgcagacuc cugaagcggg agggggugcu gcaggccgcu aauuucgacg      420

agaacggacu cauuaagucc cugcccaaua ccccuuggca acuucgcgcc gcugcccugg      480

auagaaaacu cacaccccug gaauggucug cgguccuucu gcaccucauc aagcaucggg      540

gcuaucugag ccagcgcaaa aacgagggcg aaaccgccga caaggagcug ggggcucucc      600

ugaaaggagu ggccggcaau gcucacgccc uucagacagg ggauuuuaga accccugccg      660

agcuggcucu caacaaguuc gaaaaggagu ccggacauau ucggaaucag agaucugacu      720

acagccacac auuuucccgc aaagaucugc aagcggaacu uauccuccug uucgagaagc      780

agaaagaauu uggcaacccg cacguuagcg ggggacugaa ggagggcauu gagacccugc      840

ucaugacgca gcggcccgcc cugucuggcg acgcugugca gaaaaugcuu gggcauugca      900

ccuucgaacc ugccgagccc aaggccgcua aaaauacaua uaccgccgaa agauuuaucu      960

ggcugacaaa gcucaacaau cugcgcauuc uugagcaagg auccgaacgg ccucugaccg     1020

auacggagag agcgacacuc auggacgagc ccuaccgcaa gagcaaacug accuaugcuc     1080

aggcccggaa gcugcucggc cuggaagaua cagccuucuu uaaagggcuu agauacggaa     1140

aggacaacgc ugaggccucu acccugaugg aaaugaaggc uuaucacgcc aucucccgcg     1200

cgcucgagaa ggaaggccug aaggacaaaa agagcccgcu gaaucuuucu ccugagcucc     1260

aggaugagau cgggaccgcc uucucccugu uuaaaacaga cgaagauauu acgggacggc     1320

ugaaggacag aauccagccc gagauucucg aagcucugcu uaaacauauc agcuucgaua     1380

aguucgucca aauuucccug aaggcccucc ggcgcaucgu gccucugaug gagcagggca     1440

aaagauacga cgaggcuugu gccgaaaucu acggcgauca cuaugggaag aaaaacaccg     1500

aggaaaagau uuaccugccu cccauccccg cggacgagau ucggaacccg gugguccuua     1560

gagcccucuc ucaggcucgc aaaguuauca auggaguggu gcggagauau ggcagccccg     1620

cccgcauuca caucgaaaca gcuagagagg ucgggaaguc cuuuaaggau cggaaagaga     1680

ucgaaaagcg ccaagaggaa aacagaaaag accgggagaa ggccgccgcu aaauuccgcg     1740

aauacuuucc uaauuucgug ggagagccca agagcaaaga uauucugaag cugagacucu     1800

augagcagca gcauggcaag ugccuguacu cugggaaaga aaucaaccuu ggccggcuga     1860

augagaaggg auauguugaa auugaccacg cgcucccuuu uucccgcacc ugggacgaua     1920

gcuucaacaa uaaagugcug guccugggcu cugagaacca gaauaagggg aaccaaacac     1980

ccuacgaaua cuuuaaugga aaagacaaca gcagagagug gcaggaguuc aaggcccggg     2040

uggaaaccuc ccgcuuuccg agaucuaaga aacagcggau ccuucuccag aaguucgaug     2100

aggacggcuu uaaagaaaga aaucugaacg auacgcgcua uguuaaucgg uuccuguguc     2160

aauuuguggc ugacagaaug cgccucacag ggaagggcaa aaagcggguc uucgccagca     2220

acggacagau uaccaaccug cuuagagggu ucuggggccu gcgcaaggug cgggccgaga     2280

augauagaca ucacgcucuc gacgccgucg ugguugcgug cuccaccgug gcuaugcagc     2340

agaaaaucac acgcuuuguc cgguacaagg agaugaacgc cuucgaugga aaaaccauug     2400

acaaggaaac aggcgaggug cugcaucaga aaacgcacuu uccucagccc ugggaguucu     2460

uugcccagga aguuaugauc agaguguucg ggaagccuga uggaaagccc gaauuugagg     2520

aggcugacac ccuggaaaaa cuucggacac uccuggccga gaagcugagc ucucgcccug     2580

aagcggucca cgaguaugug acaccccucu ucguguccag agcuccgaau cggaaaauga     2640

gcggccaggg gcauauggaa accguuaagu cugccaaacg ccuggaugag ggagucuccg     2700

ugcuuagagu gccucugacc caacucaagc ugaaagaccu ggagaagaug gucaaccggg     2760

aaagagagcc caaacuuuac gaagcccuga aagcucgccu cgaggcccac aaggacgauc     2820

ccgcuaaagc cuuugcggaa ccuuucuaua aguacgacaa agccggcaau cggacgcaac     2880

aggugaaggc uguuagagug gagcaagucc agaagaccgg gguguggguu cgcaaccaua     2940

auggcaucgc cgauaacgcu acaauggugc gggucgacgu guuugagaaa ggagauaagu     3000

auuaccuggu uccgauuuac agcuggcaag uggccaaagg cauccucccc gacagagccg     3060

ucgugcaggg gaaggaugaa gaggacuggc agcugauuga ugacuccuuc aauuuuaaau     3120

ucucucuuca cccuaacgau cugguggaag ucaucaccaa gaaggcucgc auguucggau     3180

auuuugcgag cugucaccgg ggcacaggga auauuaacau cagaauccau gaccucgauc     3240

acaaaauugg aaagaauggc auccuggagg ggauuggcgu uaaaaccgcc cuguccuucc     3300

agaaguacca gaucgacgag cuuggaaaag aaauucgccc cugcagacug aagaagcggc     3360

cucccgugcg caaaagaccu gcugccacga agaaagccgg ccaggcuaag aaaaagaagu     3420

aaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu ccugccaaau     3480

aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                3575


<210>  4849
<211>  4331
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4849
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gaaaccguac accauuggac uggacaucgg cacaaacagc guuggguggg      180

cggugcucac ggaucaguau gaccugguga agcggaagau gaaaauugcu ggagauuccg      240

agaagaaaca aaucaagaaa aauuucuggg gcguccgccu uuuugacgaa gggcagaccg      300

ccgccgauag acggauggcu cgcacagcca gacggcgcau ugagagacgg cgcaacagaa      360

ucucuuaccu gcagggaauc uucgcugaag agaugagcaa gaccgacgcc aauuucuucu      420

gccggcucuc cgauucuuuu uauguggaca acgaaaagcg caauagcaga caucccuucu      480

uugcgacaau ugaggaggaa guugaguacc acaaaaacua uccuaccauc uaccaucugc      540

gggaggaacu ugucaauucc ucugagaagg ccgaucugcg ccucgucuau cuggcucugg      600

cccacauuau caaauacaga ggcaacuucc ucauugaagg cgcucuggac acacagaaua      660

ccagcgugga ugggaucuac aagcaauuua uucagacgua uaaccagguu uucgccuccg      720

gaaucgagga cggcagccuu aaaaagcugg aagauaauaa agacguggcg aagauccucg      780

ucgagaaggu gacccggaaa gaaaagcugg agcgcauucu uaaacuguac cccggggaga      840

agucugccgg aauguuugcu caguucaucu cccucauugu gggcagcaaa gggaacuuuc      900

aaaagccuuu cgaccugauc gaaaagucug auauugagug ugccaaagac uccuaugaag      960

aggaucugga aagccuccug gcucuuaucg gagacgagua cgccgagcug uucgucgccg     1020

cuaagaaugc guauucugcc guugugcucu ccagcaucau uacaguggcu gaaaccgaga     1080

caaacgccaa acuguccgcc ucuaugaucg aaagauuuga uacccaugag gaagaccuug     1140

gcgagcugaa ggcuuucauu aaacuccauc ugcccaagca cuacgaggaa aucuuuagca     1200

auacggagaa gcacggcuau gccggguaca uugauggaaa aacaaagcag gcggacuucu     1260

acaaauauau gaagaugacc cuggaaaaca ucgagggcgc ugauuacuuu aucgccaaaa     1320

uugagaagga aaacuuccuc cggaagcaga gaacauuuga caauggggcc aucccucacc     1380

agcugcaucu ugaggaacug gaggcuauuc uccaccaaca ggccaaauau uacccguucc     1440

ugaaggaaaa cuaugauaaa aucaaguccc uugucaccuu ucgcauuccc uacuucgugg     1500

gaccucuggc uaauggccag agcgaguuug ccuggcucac ccggaaagcg gacggggaga     1560

ucagacccug gaacauugaa gagaagguug acuucggaaa aucugccgug gauuuuaucg     1620

aaaagaugac aaauaaggac acguaucugc cuaaagagaa cguccugccc aagcacuccc     1680

ucugcuacca aaaauaccug guguauaaug aacuuaccaa gguucgcuac aucaacgauc     1740

agggcaaaac aagcuauuuc ucuggccagg agaaggagca gauuuucaau gaccuguuua     1800

agcaaaaacg gaaggugaaa aagaaagauc ucgaacuguu ccugagaaac augagccaug     1860

ucgagucccc gaccaucgaa gggcuugagg acucuuuuaa uagcuccuac agcacauauc     1920

acgaucuccu gaagguggga auuaagcagg aaauccugga caacccuguc aauaccgaga     1980

ugcucgagaa cauugugaaa auccugacgg uuuucgaaga uaagcgcaug aucaaagagc     2040

agcuucagca auuuucugac gugcuggaug gcgucgugcu caagaaacug gaacggagac     2100

auuacacagg guggggacgc cuguccgcua agcuccucau gggcauucgg gacaagcaga     2160

gccaccugac cauccuggau uaucucauga augacgacgg gcugaacaga aaccuuaugc     2220

agcugauuaa ugauucuaac cucuccuuca aaagcaucau ugagaaggag cagguuacca     2280

cagccgacaa agauauccaa uccaucgugg cugaccuggc cggcucuccc gcgauuaaga     2340

aaggaauccu gcagagccuu aagauugucg augaacucgu guccgugaug ggcuacccuc     2400

cccagaccau cguugucgag auggcccggg aaaaucagac aacggggaaa ggaaagaaca     2460

auucucgccc cagauacaag agccuggaga aagcuauuaa ggaauuuggc ucccaaaucc     2520

ugaaagagca uccuaccgac aaccaggagc uccggaauaa cagacuguau cuuuacuauc     2580

ugcagaaugg gaaggauaug uacacaggac aagaccucga uauccacaac cugagcaauu     2640

augacauuga ucacaucgug ccgcagucuu ucauuaccga caacuccauc gauaaucugg     2700

ugcuuacaag cucugccggc aaccgcgaaa aaggggacga cgugcccccu cucgagauug     2760

ugcggaagag aaagguguuu ugggaaaaac uguaccaggg caaucugaug uccaagcgca     2820

aauucgauua ucucaccaag gcugagagag gaggccugac ggaagccgac aaagcccggu     2880

uuauccaucg ccagcuuguu gagaccagac aaauuacaaa gaacgucgcu aacauccugc     2940

accagcgguu caauuacgag aaggaugacc augggaacac caugaaacag gugcgcaucg     3000

uuacacucaa gagcgcgcug gugucucagu uuagaaaaca auuccagcug uacaaggucc     3060

gggaugugaa ugacuaucac cacgcccaug augcuuaccu uaacggaguu guggccaaua     3120

cccugcucaa agucuauccc cagcuggaac cugaguucgu guacggcgac uaucaccagu     3180

uugauugguu caaggccaac aaggcuacgg ccaaaaagca auuuuacaca aauauuaugc     3240

uuuucuuugc gcagaaagac cgcaucauug augaaaacgg ggagauccug ugggacaaga     3300

aauaucuuga caccgugaag aaggucauga gcuacagaca gaugaauauu guuaaaaaga     3360

cagaaaucca aaaaggagag uucuccaagg cuaccaucaa acccaagggc aacagcucua     3420

aacugauucc gcggaagacc aauugggauc cuaugaagua cgggggccuc gacuccccca     3480

acauggccua ugccguggug aucgaguacg cuaaaggaaa gaauaaacug gucuuugaaa     3540

agaaaauuau ccgcgugaca auuauggaga gaaaggccuu cgaaaaggau gagaaagcgu     3600

uucuggagga acagggcuau cggcagccua agguucuugc uaaacugccc aaguacacgc     3660

ucuaugagug ugaagagggg agacgccgga ugcuggccag cgccaacgaa gcucagaaag     3720

gaaaucaaca ggugcucccu aaccaucugg ucacccuucu gcaccaugcc gcuaacugcg     3780

agguguccga cggcaagucu cucgauuaca ucgagagcaa uagagaaaug uucgccgagc     3840

ugcuggcgca cguuuccgaa uuugccaagc gcuauacacu ugcugaggcc aaccugaaua     3900

aaaucaacca gcucuuugaa cagaauaagg agggggacau uaaagcuauc gcccaaucuu     3960

ucguggaucu gauggccuuu aacgcuaugg gagcgcccgc cagcuucaag uuuuucgaga     4020

ccacaauuga acggaaaaga uacaauaacc ucaaggagcu gcuuaauucc accaucauuu     4080

accagucuau cacgggccug uaugaaagcc gcaagcggcu ggacgauaaa agaccggcug     4140

ccacaaagaa agccgggcag gcuaagaaaa agaaauaagg acuagugucc accugucccu     4200

ccugggcugc uggauugucu cguuuuccug ccaaauaaac aggaucagcg cuuuacagau     4260

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaagaau u                                                          4331


<210>  4850
<211>  4364
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4850
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa gaaaccguac agcauuggac uggacaucgg caccaacucc guuggguggg      180

cgguggugac agaugacuau aagguccccg cuaagaaaau gaaggugcuc ggaaauacgg      240

auaaaucuca uauugagaag aaccugcuug gcgcccuccu guucgacagc gggaauaccg      300

ccgaagaucg gcgccugaaa agaacagcuc ggcgcagaua cacccggcgc agaaaccgga      360

uccuuuaucu gcaggagauu uucuccgaag agaugggaaa gguugacgau ucuuucuuuc      420

accgccucga agacagcuuc cuggugacag aggauaagag aggcgagcgg cauccuaucu      480

uuggcaaucu ggaagaggaa gucaaauacc acgagaacuu ccccaccauc uaucaucucc      540

gccaauaccu ggccgacaau ccugagaagg uggaucuuag acugguuuau cucgcucugg      600

cccacauuau caaauuucgg gggcacuucc uuauugaagg aaaguuugac acacgcaaca      660

augaugugca gagacuguuc caggaguuuc ucgcggucua cgacaacacc uucgaaaauu      720

ccucucugca ggagcaaaac guccaggugg aagagauccu gacggacaaa auuagcaagu      780

ccgccaaaaa ggaucgggug cucaagcugu uucccaauga gaaaagcaac ggcagauucg      840

cugaauuucu uaagcugauc guugggaauc aggccgacuu uaaaaagcau uucgagcucg      900

aagagaaagc uccucugcag uuuucuaagg auaccuacga agaggagcuu gaagugcugc      960

ucgcccaaau uggagacaac uaugcggagc uguuccuguc cgccaagaaa cucuacgaua     1020

gcauccugcu uucuggcauc cugacaguga ccgacgucgg gacaaaggcu ccgcucuccg     1080

ccagcaugau ucagcgcuau aacgaacacc agauggaucu ggcucagcuu aaacaauuua     1140

uccggcagaa gcugucugac aaauacaaug agguguucuc cgauguuagc aaggacggau     1200

augccggcua cauugauggc aagaccaacc aggaagccuu uuauaaauac cucaaggggc     1260

ugcugaauaa aaucgaggga uccggcuacu uccucgacaa gauugagaga gaagacuuuc     1320

ugcgcaaaca acggacguuc gauaacgggu cuauccccca ucagauccac cuucaggaga     1380

ugagagcuau uauccgccgg caggcggaau uuuauccuuu ccuggccgac aaucaagaua     1440

gaauugagaa gcuccugaca uuucgcaucc ccuacuaugu gggaccucug gcucggggca     1500

agagcgacuu cgccuggcuu uccagaaaaa gcgccgauaa gauuaccccc uggaacuucg     1560

acgagaucgu cgauaaagaa ucuuccgcug aggccuuuau caaucgcaug acaaacuacg     1620

accucuaucu gccgaaucag aaggugcugc cuaaacacag ccuccuguac gaaaaguuca     1680

ccguuuauaa cgagcuuacc aaagugaagu acaagacaga acaggggaaa acggcguuuu     1740

ucgaugcuaa uaugaagcag gagauuuuug acggagucuu caaaguguac cggaagguca     1800

ccaaagauaa gcugauggac uuucucgaga aggaguucga ugaauuuaga aucguggacc     1860

ugacaggccu ggacaaagaa aacaagguuu ucaaugccuc uuauggcacc uaccaugauc     1920

uuugcaaaau ucucgacaag gauuuucugg acaacagcaa aaaugagaag auccuggaag     1980

auauugugcu cacacugacc cuuuucgagg accgggagau gauccgcaag agacuggaaa     2040

acuauuccga ucuccugacg aaagagcaag ucaagaaacu ggaacggaga cacuacacag     2100

gguggggacg ccuuucugcc gagcucauuc auggcauccg gaacaaggaa agcagaaaaa     2160

ccauccugga cuaucugauu gaugacggaa auuccaaccg caauuuuaug cagcucauca     2220

acgaugacgc ucugagcuuc aaggaggaga uugccaaggc ucaggugauc ggggaaaccg     2280

auaaucuuaa ccagguugug ucugacauug ccggcucccc cgcgaucaaa aaggggaucc     2340

ugcaaagccu caaaauuguc gacgagcugg ugaagaucau gggccaccag ccugaaaaua     2400

uugugguuga gauggccaga gagaaccagu ucacaaauca gggacggcgc aacucucaac     2460

agagacugaa aggccuuacc gauuccauca aggaauuugg gagccagauu cucaaagagc     2520

aucccgucga aaauucccaa cugcagaacg accggcuguu ccucuacuau cugcagaaug     2580

gacgcgauau guacacaggc gaggaacuug acaucgauua ccugucucag uaugacaucg     2640

aucacauuau cccccaggcu uuuauuaagg acaacagcau cgauaauaga gugcucacgu     2700

ccucuaagga gaaccggggg aaaagcgacg augugccuuc caaggacguc gugcgcaaaa     2760

ugaaaagcua cuggucuaaa cugcuguccg ccaaacuuau uacccaaaga aaguucgaca     2820

accugacaaa agcugagcgg ggaggccuca ccgaugacga uaaggccggg uuuaucaaac     2880

gccagcuggu ugaaacaaga cagaucacca agcacguggc gcggauucuc gacgagagau     2940

ucaauacgga aaccgaugag aacaauaaaa agauccgcca agucaagauu gugacacuga     3000

aaagcaaccu uguuucuaau uuucggaagg aguucgaacu guauaaagug agagagauca     3060

acgacuacca ucacgcccau gaugcuuauc ucaaugccgu cauuggcaag gcucugcugg     3120

gaguguaccc gcagcuugaa cccgaguuug uuuauggcga cuacccucac uuccacgggc     3180

auaaagaaaa caaggccacc gccaagaaau uuuucuacuc caauaucaug aacuucuuua     3240

agaaagauga cgugcgcaca gauaagaaug gagagauuau cuggaaaaag gacgaacaca     3300

ucagcaacau uaagaaaguc cugucuuauc cccaggugaa uaucgugaag aaagucgagg     3360

agcagaccgg cggguucagc aaggaaucca uucucccuaa aggaaacagc gauaagcuga     3420

ucccccggaa aacgaagaag uuuuacuggg acacaaagaa guauggcggg uucgacucuc     3480

cgauuguugc uuacuccauc cucgugaucg cggauauuga gaaaggcaag agcaaaaagc     3540

ugaagaccgu gaaagcccuu gucggaguga caaucaugga aaagaugacc uuugagagag     3600

acccuguugc uuuccuggag cgcaaaggcu aucggaaugu gcaagaagag aacauuauca     3660

agcuccccaa auacucccug uuuaagcugg aaaacgggag aaagcgccuu cuggccucug     3720

cccgggagcu ccagaaagga aaugaaauug uccugccuaa ccaucucggc acccugcuuu     3780

aucacgcuaa gaauauccau aaaguggaug agcccaagca ccuggacuac guugauaaac     3840

acaaggacga guucaaggaa cugcucgaug uggucagcaa cuuuuccaaa aaguacacac     3900

uggccgaggg gaaucuugaa aagaucaagg agcuguaugc gcagaacaau ggagaagacc     3960

ucaaagagcu ggcuucuagc uucauuaacc uccugacguu uaccgccauc ggcgccccug     4020

cuacauucaa guuuuucgau aaaaauauug acagaaagcg guacaccucc acaaccgaga     4080

uccuuaacgc cacgcugauu caucaaucua ucacagggcu guaugaaacc cgcauugauc     4140

ucaauaagcu gggcggagac aaaagacccg cggcuaccaa gaaagccggc caggccaaga     4200

aaaagaagua aggacuagug uccaccuguc ccuccugggc ugcuggauug ucucguuuuc     4260

cugccaaaua aacaggauca gcgcuuuaca gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     4320

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aauu                      4364


<210>  4851
<211>  3497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4851
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccca gacaacaaac cugagcuaca uucucggacu ggaccuuggc aucgcguccg      180

uugggugggc ugugguggag auuaaugaaa acgaggaucc gaucggacug auugacgucg      240

gcgugcggau cuucgaacgc gccgagguuc ccaagacggg ggaaucucuc gcccugagca      300

gacggcuugc ucgcuccacc agacggcuga uccgcagacg ggcccaucgc cuccugcugg      360

cuaaaagauu ucucaagcgg gaggggauuc ugucuacaau cgaucuugag aagggccugc      420

cuaaucaagc cugggaacuc cgcguggcgg gccuggagag acggcuuagc gccauugaau      480

ggggagcugu ccugcuccac cugaucaaac aucgcggaua ucuguccaag cggaaaaacg      540

agucucagac caauaacaag gagcucggcg cccugcuuag cgggguggcu cagaaucacc      600

agcugcucca auccgacgau uacagaacac ccgccgaacu ggcgcuuaaa aaguucgcca      660

aggaggaagg acauauuaga aaccagcgcg gcgcuuauac ccacacauuu aaucggcugg      720

accuccuggc cgagcugaac cuccuguucg cucagcagca ccaauuuggg aauccucauu      780

gcaaagaaca cauccagcag uacaugaccg agcuucugau guggcagaag cccgcccuca      840

gcggagaggc cauucugaaa augcuuggca aguguacgca ugaaaagaac gaguucaaag      900

cugcgaaaca caccuauucu gccgaaagau uuguuuggcu gacaaagcuc aauaaccugc      960

gcauccugga ggauggcgcu gaacgggccc ucaaugagga ggaaagacaa cugcuuauca     1020

accacccuua cgagaagucc aaacugaccu augcccaggu gcgcaagcuc cuggggcuga     1080

gcgaacaggc uauuuucaaa caucuucggu acucuaagga gaacgccgaa uccgcgacau     1140

uuauggagcu caaggcuugg cacgccauca gaaaagcccu ggagaaucaa ggacugaagg     1200

acaccuggca ggaucucgcu aaaaagccgg accugcuuga ugaaauuggc acggccuuca     1260

gccuguacaa aacagacgag gauauccagc aguaucucac caacaagguc cccaauucug     1320

ugauuaacgc ucugcuggug ucccuuaauu uugacaaauu caucgaacuc agccugaagu     1380

cccugcgcaa gauccucccu cugauggagc aagggaaacg guacgaccag gccugcagag     1440

agauuuaugg acaucacuac ggcgaagcga accagaagac aucucagcuu cugcccgcca     1500

ucccugcuca agagauucgc aaucccgucg uucuccggac ccugagccag gccagaaaag     1560

ugaucaacgc uauuauccgg caguaugggu ccccggcccg cgugcauauc gaaaccggaa     1620

gagagcuggg caagagcuuu aaagaacgga gagagauuca gaagcaacag gaggauaauc     1680

gcacaaagcg ggaaucugcg guccagaaau ucaaggagcu uuucuccgac uuuagcucug     1740

aaccuaaaag caaggauauc cucaaauuca gacuguacga gcagcaacac ggcaaguguc     1800

uguauuccgg gaaagaaauu aacauccacc gccucaauga gaagggauac guggagauug     1860

accaugcccu gcccuuuucu agaacguggg augacagcuu caacaauaag guucuugugc     1920

uggcuuccga aaaccagaau aaaggcaacc agaccccuua cgaguggcuc caagggaaga     1980

ucaacagcga acgguggaaa aauuuugucg cccuggugcu gggaucucag ugcuccgcug     2040

ccaagaaaca gcgccuucuc acacagguua uugaugacaa caaguucauc gauagaaauc     2100

ugaacgacac ccgguauauc gcccgcuuuc ugagcaauua cauucaagag aaccuccugc     2160

uugugggcaa gaauaaaaag aacgucuuca cacccaaugg gcagaucacc gcucugcuca     2220

gaucucggug gggccugauu aaagcgcgcg aaaacaauaa cagacaccau gcccuggaug     2280

cuaucguggu cgccugugcc acgcccucca ugcagcagaa gauuacacgg uuuauccgcu     2340

ucaaagaggu gcacccuuau aagaucgaga auagauacga aaugguugac caagagagcg     2400

gagaaauuau cuccccgcac uuucccgagc cuugggcuua uuuccggcag gaggugaaca     2460

uuagagucuu ugacaaucau cccgauaccg ugcuuaagga aaugcugccu gaccgccccc     2520

aggccaacca ccaguucguu caaccucucu ucgugucucg ggcgccgacc agaaaaauga     2580

gcggccaggg gcauauggag acaaucaagu ccgcuaaacg ccuggccgaa ggaauuucug     2640

uccuccggau cccccugacc cagcuuaagc cuaaccugcu cgagaauaug gugaacaaag     2700

aaagagagcc cgcccuguac gcuggccuga aggcccgccu ugcggaguuu aaucaagauc     2760

cugcuaaggc cuucgccaca cccuuuuaua aacaaggggg acaacaggug aaggcuaucc     2820

ggguugaaca gguccagaaa agcggcgugc uggugagaga gaacaauggg gucgccgaca     2880

acgcuuccau ugugcgcacg gauguuuuca ucaagaauaa caaauuuuuc cucgugccga     2940

uuuacaccug gcaggucgcc aagggcaucc ugccuaauaa agcgauugug gcccacaaga     3000

acgaagacga gugggaagag auggaugagg gagcuaaguu uaaauucagc cucuuuccca     3060

augaccuggu ugaacuuaag accaaaaagg aguacuucuu uggcuauuac aucgggcugg     3120

aucgggccac aggaaacauc ucucucaaag aacaugacgg cgagauuucc aaggggaagg     3180

auggagugua uagagucggc gugaaacugg cucugagcuu cgaaaaguac cagguugacg     3240

agcuugggaa aaaucggcag aucugccgcc cccagcagag acagccugug cggaagcgcc     3300

cggccgccac caaaaaggcu ggccaagcga agaaaaagaa auaaggacua guguccaccu     3360

gucccuccug ggcugcugga uugucucguu uuccugccaa auaaacagga ucagcgcuuu     3420

acagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3480

aaaaaaaaaa aagaauu                                                    3497


<210>  4852
<211>  3575
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4852
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc ggcuuucaag ccgaacccca uuaauuacau ccugggacuc gacauuggca      180

ucgccagcgu ugggugggcc augguggaga uugaugaaga cgagaacccu aucugccuga      240

ucgaucuugg agugcggguc uuugaacgcg cugaggugcc caaaaccggc gacucccugg      300

ccauggcuag acggcucgcc cgcucuguua gacggcugac acgcagacgg gcgcaucgcc      360

uucugagagc ccggcgccuc cugaagagag aaggggugcu gcaggcugcc gauuucgacg      420

agaauggacu cauuaagagc cugccuaaca cgcccuggca acuucgggcu gccgcgcugg      480

aucgcaaacu caccccucug gagugguccg ccguccuucu gcaccucauc aagcauagag      540

gcuaucuguc ucagcggaaa aaugaaggcg agacagcuga caaggaacug ggggcccucc      600

ugaaaggagu ggcugauaac gcccacgccc uucagaccgg cgacuuuaga acaccggcug      660

agcuggcgcu caauaaguuc gagaaggaaa gcgggcauau ucgcaaccag cggggagauu      720

acucccacac cuuuucuaga aaagaccugc aagccgagcu uauccuccug uucgaaaagc      780

agaaagaguu uggcaauccc cacguuagcg ggggacugaa ggaaggcauu gagacacugc      840

ucaugaccca gcgcccugcu cuguccggcg augccgugca gaaaaugcuu gggcauugua      900

cguucgagcc cgccgaaccu aaggcugcca aaaacaccua uacagcggag cgguuuaucu      960

ggcugaccaa gcucaauaac cugcgcauuc uugaacaagg aagcgagaga ccccugacag     1020

acaccgaacg ggcuacgcuc auggacgagc cguacagaaa gucuaaacug acauaugccc     1080

aggcccgcaa gcugcucggc cuggaggaua ccgcuuucuu uaaagggcuu cgguacggaa     1140

aggacaaugc cgaggcuucc acacugaugg aaaugaaagc cuaucacgcg aucagcagag     1200

cccucgaaaa ggagggccug aaggauaaaa agucuccucu gaaccuuucc cccgaacucc     1260

aggacgagau cgggaccgcu uucagccugu uuaaaaccga ugaggacauu acaggacgcc     1320

ugaaggaucg gauccagccu gaaauucucg aggcccugcu uaaacauauc ucuuucgaca     1380

aguucgucca aauuucccug aaggcucuca gacggaucgu gccccugaug gaacagggca     1440

aacgcuacga ugaggccugc gcggagaucu acggcgacca cuaugggaag aaaaauacgg     1500

aagagaagau uuaccugccc ccuaucccgg ccgaugaaau uagaaacccc gugguccuuc     1560

gggcucucag ccaggccaga aaaguuauca acggaguggu gcgccgguau ggcuccccug     1620

cucgcauuca caucgagacc gccagagaag ucgggaaguc uuuuaaggac agaaaagaga     1680

ucgagaagcg gcaagaagag aaucgcaaag acagagaaaa ggccgcugcg aaauuccggg     1740

aguacuuucc caacuucgug ggagaaccua agagcaaaga uauucugaag cugcgccucu     1800

augagcagca gcauggcaag ugucuguacu ccgggaaaga gaucaaucuu ggcagacuga     1860

acgaaaaggg auauguugag auugaccacg cccuccccuu uagccggaca ugggaugacu     1920

cuuucaauaa caaagugcug guccugggcu ccgaaaauca gaacaagggg aaucaaaccc     1980

cguacgagua cuuuaacgga aaagauaaua gccgcgaaug gcaggaguuc aaggcuagag     2040

uggagacauc ucgguuuccu cgcagcaaga aacagagaau ccuucuccag aaguucgacg     2100

aagauggcuu uaaagagcgg aaccugaaug acaccagaua uguuaaccgc uuccugugcc     2160

aauuuguggc cgaucggaug agacucacgg ggaagggcaa aaagcgcguc uucgccucca     2220

auggacagau uacaaaccug cuucgggggu ucuggggccu gagaaaggug cgcgcugaaa     2280

acgaccggca ucacgcccuc gaugcggucg ugguugcuug uucuaccgug gccaugcagc     2340

agaaaaucac cagauuuguc cgcuacaagg agaugaaugc cuucgacgga aaaacaauug     2400

auaaggagac cggcgaagug cugcaucaga aaacacacuu uccccagccu ugggaguucu     2460

uugcucagga aguuaugauc cggguguucg ggaagcccga cggaaagccu gaguuugaag     2520

aggccgacac gcccgagaaa cugagaaccc uucucgcgga aaagcugagc ucccggccgg     2580

aggcugucca cgaauaugug acaccucugu ucgugagccg cgcccccaac agaaaaaugu     2640

cuggccaggg gcauauggag accguuaagu ccgccaaacg gcucgaugaa ggagucagcg     2700

ugcugcgcgu gccccuuaca caacugaagc ucaaagaccu ggagaagaug gucaauagag     2760

agcgggaacc uaagcuguac gaggcucuua aagccagacu ggaagcucac aaggaugacc     2820

cggccaaagc guuugccgag cccuucuaua aguacgauaa agcuggcaac cgcacccaac     2880

aggugaaggc cguucgggug gaacaagucc agaagacggg gguguggguu agaaaucaua     2940

acggcaucgc ugacaaugcc accauggugc gcgucgaugu guuugagaaa ggagacaagu     3000

auuaccucgu uccuauuuac ucuuggcaag uggccaaagg cauccugccc gaucgggcug     3060

ucgugcaggg gaaggacgag gaagauuggc agcucauuga cgauuccuuc aacuuuaaau     3120

ucagccugca cccuaaugac cuuguggagg ucaucacaaa gaaggcgaga auguucggau     3180

auuuugccuc cugccaccgc ggcaccggga acauuaauau ccggauccau gaccuggauc     3240

acaaaauugg aaagaacggc auccucgaag ggauuggcgu uaaaacagcu cugucuuucc     3300

agaaguacca gaucgacgag cugggaaaag agauuagacc cugucgccuu aagaagagac     3360

cucccgugcg gaaacgcccg gccgccacca agaaagcugg ccaggccaag aaaaagaagu     3420

aaggacuagu guccaccugu cccuccuggg cugcuggauu gucucguuuu ccugccaaau     3480

aaacaggauc agcgcuuuac agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gaauu                                3575


<210>  4853
<211>  3581
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4853
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccaa guaccauguu ggaauugacg ugggcaccuu cagcgugggg cuggcggcua      180

ucgaggucga ugacgccgga augccgauua aaacacucuc ccuggugucu cacauccaug      240

auagcggccu ugaccccgau gaaauuaagu ccgccguuac gcggcuggcu ucuagcggga      300

ucgcccgcag aacccggcgc cucuauagac ggaagcgcag acggcugcag caacuugaca      360

aauuuaucca gcgccaggga uggccuguga uugagcugga agauuacucc gacccccucu      420

auccuuggaa ggucagagcu gagcuggccg cgucuuacau cgccgaugaa aaagagcggg      480

gcgagaagcu gagcguggcu cuccgccaca uugccagaca ucggggcugg cgcaaccccu      540

augcuaaagu uuccagccug uaccuuccug acgggccguc ugaugccuuc aaggcgauca      600

gagaagagau uaagcgggcc uccggacagc ccgugccuga aacagcuacc gucggccaaa      660

uggugacacu gugcgagcuc gggacccuga aacuuagagg agagggcggg gugcugagcg      720

cccgccucca gcagucugac uaugcucggg aaauccagga gauuuguaga augcaagaaa      780

ucggacagga gcuguaccgc aagaucauug augucguuuu ugccgccgaa ucccccaaag      840

gcagcgcuuc uucccgggug ggcaaggacc cucugcagcc cgggaaaaau agagcgcuca      900

aggccagcga cgcuuuccag cgcuaccgga ucgccgcccu gauuggaaac cuuagagugc      960

gcgucgaugg cgagaaacgg auccuguccg uggaggaaaa gaaucucguu uuugaccacc     1020

uggugaaccu uacaccgaag aaagagccug aaugggucac cauugcugag auccugggga     1080

ucgauagagg acaacucauu ggcacggcca ccaugacaga cgauggggaa cgcgcgggag     1140

cucggccccc uacccacgac acaaauagau cuaucgugaa cagccggauu gcuccccugg     1200

uugauuggug gaagaccgcc uccgcccugg agcagcaugc cauggugaaa gcucucagca     1260

augccgaggu cgacgauuuc gacucucccg aaggcgcgaa ggugcaggcc uuuuucgcug     1320

aucuggacga cgauguccac gccaaacuug acucccugca ucucccugug ggccgcgcug     1380

ccuauagcga ggauacgcug guuagacuua cacggagaau gcugucugac gggguggauc     1440

ucuacaccgc gcgccugcaa gaauuuggaa ucgagccgag cuggacaccc ccuacacccc     1500

ggauuggcga accugucggg aaccccgccg uggacagagu ucugaagacc gugucccgcu     1560

ggcucgaguc ugcuaccaag acguggggag ccccggagag agucaucauc gaacacgugc     1620

gggagggcuu cgugaccgaa aaacgcgcua gagagaugga uggggacaug cggcgcagag     1680

ccgcccggaa ugcuaagcug uuucaggaga ugcaggaaaa acuuaacguu cagggcaagc     1740

cuagccgcgc ggaucugugg agauaucaau ccguccagcg gcagaauugc cagugugccu     1800

acugcggaag ccccauuaca uucucuaacu ccgagaugga ccacaucgug ccucgcgcug     1860

gccaagggag caccaauaca agagaaaacc ucguggccgu cugucaucgg ugcaaucagu     1920

cuaaaggaaa cacccccuuu gccauuuggg cuaagaacac guccaucgag ggcgugagcg     1980

uuaaggaagc cguggagaga acacgccacu gggucaccga uaccgggaug cgguccacag     2040

acuucaaaaa guucaccaaa gcggugguug agagauuuca gcgcgcuaca auggaugaag     2100

agauugacgc ccggucuaug gaaagcgugg ccuggauggc uaaugagcug agaucccgcg     2160

ucgcccagca uuucgcgucu cacggaacga ccgugcgggu uuauagaggc agccugacag     2220

cugaagcccg ccgggccucc gggaucagcg gcaagcugaa auuuuucgac ggagugggca     2280

agucuagacu cgaucggcgc caucacgcua uugacgccgc ugucaucgcc uuuaccuccg     2340

auuacguggc ggagacacug gccgugagaa gcaaccugaa gcaaucucag gcucaccggc     2400

aggaggcccc ucaguggcgc gaauuuaccg ggaaagacgc ugagcauaga gccgccuggc     2460

gggucuggug ucaaaagaug gaaaaacucu ccgcucugcu uacggaggau cugagagacg     2520

aucgcguugu ggugaugagc aauguccggc ucagacuggg aaacggcucu gcgcacaagg     2580

aaaccaucgg gaaacugagc aaggugaaac uuuccagcca gcucucuguu uccgacauug     2640

auaaggccag cuccgaggcu cuguggugcg cccugacacg cgagcccgga uuugacccga     2700

aggaaggccu cccugccaau cccgagcggc auaucagagu gaacgggacc cacgucuaug     2760

cuggcgauaa uauuggacug uuccccgugu cugccggcag caucgcgcuu cgcgggggau     2820

acgcugaacu gggcuccucu uuucaccaug cccggguuua caaaauuaca agcgggaaga     2880

aaccugccuu cgcuaugcuc agaguguaua ccaucgaccu gcugccguac cgcaaccagg     2940

aucuuuucuc cgucgagcuc aagccccaaa cgaugucuau gagacaggcc gagaaaaagc     3000

ugcgggacgc gcuggcuaca ggaaaugccg aauaucucgg cuggcuggug guugacgaug     3060

agcuuguggu cgacaccagc aagaucgcca cagaucaggu gaaagcugug gaagccgagc     3120

uggggaccau ucgcagaugg cgggucgacg gcuucuuuuc cccuagcaag cuccgccuga     3180

gaccccugca gaugucuaaa gaaggaauca agaaagaguc cgcuccugag cuuagcaaga     3240

uuaucgaucg gcccggcugg cucccugccg uuaacaagcu guucucugac gggaauguga     3300

ccguggucag acgcgauucc cugggacggg ugagacucga aagcacagcg caccuucccg     3360

uuacguggaa agugcaaaag cgcccggccg cuaccaaaaa ggccggccag gcuaaaaaga     3420

agaaauaagg acuagugucc accugucccu ccugggcugc uggauugucu cguuuuccug     3480

ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3581


<210>  4854
<211>  3281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4854
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccgc gcggauucug gcuuucgaca ucggaauuag cuccaucggc ugggccuuuu      180

cugagaacga ugaacucaag gacugcgggg uucgcauuuu caccaaagug gagaauccga      240

agacaggaga aagccuggcc cuucccagac ggcuggcucg cuccgccaga aagcggcucg      300

cucgcagaaa agcccggcug aaccaucuua agcaccugau cgcgaaugag uuuaaacuca      360

acuacgaaga uuaucagucu uucgacgaga gccuggccaa ggcuuacaaa ggcucccuga      420

ucucuccuua ugagcuccgc uuuagagccc ugaaugaacu ucugagcaag caagauuucg      480

cucgggugau ucuccauauc gccaagcgca gaggguacga cgauauuaaa aacuccgacg      540

auaaggagaa aggagcgauc cugaaggcca uuaaacagaa ugaagagaaa cuugcuaacu      600

aucagagcgu cggcgaguac cuguauaaag aauacuuuca gaaguucaag gaaaauucua      660

aagaauuuac gaacgugcgg aauaagaaag aguccuacga acgcuguauc gcccagagcu      720

uccucaagga cgagcugaaa cugauuuuua agaagcaaag agaguucggc uuuucuuucu      780

ccaaaaaguu cgaagaggaa guucucagcg uggcuuuuua uaaacgggcc cugaaggauu      840

ucucucaccu ugucgggaac ugcuccuuuu ucaccgacga gaaaagagcc cccaagaaua      900

gcccucuggc uuuuauguuc guggcgcuca cacgcaucau caaccugcuu aauaaccuga      960

agaacaccga aggcauucuc uacacaaaag augaccugaa ugcccugcuc aacgagguuc     1020

ugaagaaugg aacccuuaca uauaaacaga ccaagaaacu gcucgggcug uccgacgauu     1080

acgaguuuaa gggagaaaag ggcacguauu ucaucgaguu uaaaaaguac aaagaguuca     1140

uuaaggcucu uggggagcau aaccugucuc aggacgaucu caaugaaauc gccaaagaca     1200

uuacccugau caaggaugag aucaaacuga agaaggcccu cgcuaaauau gaccugaacc     1260

aaaaucagau ugauagccuu uccaagcugg aguuuaaaga ccaccucaac aucagcuuca     1320

aggcccugaa acuggugaca ccccuuaugc ucgaaggaaa gaaguacgau gaggcgugua     1380

augaacugaa ccugaaaguc gcuauuaaug aggacaagaa agauuuucuc ccugccuuca     1440

acgagaccua cuauaaggac gaagugacaa auccgguggu ccugcgggcc aucaaagagu     1500

acagaaaggu ucuuaacgcu cugcucaaga aauauggcaa ggugcacaaa auuaauaucg     1560

aacuggcccg cgaggugggc aagaaccauu cucagcgggc uaaaaucgaa aaggagcaga     1620

acgagaauua caaggccaaa aaggacgcgg aacuggagug cgaaaaacuu gggcucaaga     1680

uuaacuccaa aaauauccug aagcugagac ucuucaaaga gcaaaaggaa uuuugugccu     1740

auagcggaga gaagauuaaa aucucugauc ugcaggacga gaagaugcuu gaaauugauc     1800

acaucuaccc cuauagccgc uccuucgacg auucuuacau gaacaaaguc cugguguuua     1860

ccaagcagaa ucaagagaaa cucaaccaga cgccuuucga ggcuuuuggc aaugacagcg     1920

ccaaguggca gaagauugaa guucuggcua aaaaccugcc cacaaagaaa cagaagcgga     1980

uccuugauaa aaauuacaag gacaaggaac aaaagaacuu caaggauaga aaucucaacg     2040

acacccgcua uaucgcccgg cuggugcuga auuacacaaa agauuaucuc gacuuucugc     2100

cucuuuccga ugacgagaac accaagcuga augacaccca gaaagggagc aagguccaug     2160

uggaggcgaa gucuggaaug cucacauccg cccugagaca cacguggggc uucagcgcua     2220

aagaucgcaa caaccaucug caccacgcca uugacgcugu uaucauugcc uacgccaaua     2280

acucuaucgu gaaggcuuuu uccgauuuca aaaaggaaca ggagagcaau uccgcggaac     2340

uuuaugccaa aaagauuucu gagcucgacu acaagaacaa acggaaguuu uucgagcccu     2400

uuagcggguu cagacagaaa guccuggaua agaucgacga aaucuucgug uccaaaccgg     2460

agcggaagaa accuucugga gcucugcaug aagagaccuu ucgcaaggaa gaggaguucu     2520

aucaaagcua cggcggcaag gaaggggucc ucaaagcccu ggagcuugga aagauuagaa     2580

aagugaaugg caagaucguu aaaaacgggg auauguuucg gguggacauu uucaagcaca     2640

agaaaacaaa uaaguuuuac gccgucccca ucuauaccau ggauuucgcu cugaaagugc     2700

ucccuaacaa ggccguugcg agauccaaaa agggagaaau uaaggacugg auccugaugg     2760

augagaauua cgaauuuugc uucagccugu auaaagacuc ucuuauccug auucagacaa     2820

aggauaugca ggagcccgag uuuguguacu auaacgcuuu caccuccagc acggucucuc     2880

ucaucguguc caaacaugac aauaaguuug aaacacugag caaaaaccag aagauucucu     2940

ucaagaaugc caacgagaaa gaagugaucg ccaagucuau uggcauccaa aaucugaaag     3000

uuuucgagaa guacaucguc agcgcucuug gggaagugac caaagccgag uuucgccagc     3060

gggaggacuu caagaaaaag agacccgcgg cuaccaagaa agccggccag gccaagaaaa     3120

agaaauaagg acuagugucc accugucccu ccugggcugc uggauugucu cguuuuccug     3180

ccaaauaaac aggaucagcg cuuuacagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaau u                         3281


<210>  4855
<211>  3692
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant mRNA product

<400>  4855
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccag cgaccugguu cucggacugg auauuggcau cggguccgug ggagugggca      180

uucuuaacaa ggucaccggg gagaucauuc auaaaaauuc ucggaucuuc ccggcggcuc      240

aggccgaaaa caaucuggug cgcagaacaa accggcaagg acgcagacuc gcccggcgca      300

agaagcacag acggguucgc cugaauagac uuuuugagga aagcggccug aucacggacu      360

ucaccaaaau uuccaucaac cucaaucccu accagcugcg ggugaagggc cugacagaug      420

agcucucuaa cgaggagcug uuuauugcuc uuaaaaauau ggucaagcau cgcgggauca      480

gcuaucugga cgaugccucc gacgauggaa acucuagcgu gggcgacuac gcucagauug      540

uuaaagaaaa uuccaagcag cucgaaacca agacaccugg gcaaauccag cuggagagau      600

aucagaccua cggacagcuu cggggcgauu ucacagugga aaaagacggg aagaaacacc      660

gccugauuaa cgucuuuccc accagcgccu auagaucuga ggcgcuccgg auccugcaaa      720

cgcagcagga guucaauccu cagaucaccg augaauuuau uaacagauac cuggagaucc      780

ucacaggaaa gcgcaaauau uaccauggcc ccggcaauga aaagucccgg accgacuacg      840

ggagauaucg cacaagcgga gagacccugg auaacauuuu cggcauccuu auugggaaau      900

gcacguuuua cccugacgag uuccgggccg cuaaggccuc uuauacagcu caagaauuua      960

accugcucaa ugaccugaac aaucuuaccg ugccgacaga gaccaagaaa cuguccaagg     1020

aacagaaaaa ccagaucauc aauuacguga agaacgagaa agccauggga cccgcgaaac     1080

ucuucaagua uauugccaaa cugcugagcu gugaugucgc ugacaucaag ggcuacagaa     1140

uugauaaauc ugggaaggcc gaaauccaca ccuuugaggc uuaucgcaaa augaagacac     1200

ucgaaacgcu ggacauugag caaauggauc gggagacccu ugacaagcug gccuacguuc     1260

ucacacugaa uaccgaaaga gagggaaucc aggaagcccu ugagcacgag uucgcugaug     1320

gcuccuucag ccagaaacag guggacgaac uggugcaauu ucgcaaggcg aacuccucua     1380

ucuucggcaa aggguggcau aauuuuagcg ucaagcucau gauggagcug auuccugaac     1440

uguacgagac auccgaggaa cagaugacca uccucacgcg gcugggaaaa cagaagacaa     1500

ccucuucuuc caacaagacc aaauauauug augagaaacu ucugacagaa gagaucuaca     1560

aucccguggu ugccaagagc gugagacagg cuauuaagau cgucaacgcc gccauuaaag     1620

aauauggcga cuucgauaau aucgugaucg agauggcucg cgaaaccaac gaggacgacg     1680

agaagaaagc cauucaaaag auccagaagg cgaauaaaga ugaaaaggac gcugccaugc     1740

ucaaagccgc uaaccaguac aacgggaagg ccgagcugcc ucacucuguu uuucauggac     1800

acaaacagcu ggcuacaaag auucggcuuu ggcaccaaca gggcgaaaga ugccucuaua     1860

cggggaagac caucagcauu caugaucuga ucaauaacuc caaucaguuc gagguggacc     1920

acauccugcc ccucucuauu acauuugaug acagccuggc caacaaaguc cuuguguacg     1980

cgaccgccaa ucaggaaaag ggccaacgga caccguauca ggcucuggau uccauggacg     2040

augccuggag cuuccgcgag cucaaggcuu uugucagaga gucuaagacc cuguccaaca     2100

aaaagaagga auaccugcuu acggaggaag acaucagcaa auucgaugug cggaagaaau     2160

uuauugagag aaaucucguu gacacccgcu acgccucucg ggugguccug aacgcgcugc     2220

aggaacauuu cagagcccac aagaucgaua caaaaguguc cguugugcgc ggacaauuua     2280

ccagccagcu cagacggcau uggggcauug agaagacacg cgacaccuau caccaccaug     2340

cugucgacgc ccugaucauc gcugccuccu cucagcuuaa ucuguggaag aaacagaaga     2400

acacgcucgu gagcuacucc gaggaucaac ugcuggacau ugaaacaggg gagcuuaucu     2460

cugaugacga auauaaagag agcguguuca aggccccuua ccagcacuuc guugauaccc     2520

ucaaauccaa ggaguuugaa gacagcauuc uguucucuua ucaggucgau uccaaauuua     2580

auagaaagau cagcgacgcu acaauuuacg cgacccggca ggccaaggug ggaaaagaua     2640

aggcugacga gaccuaugug cugggcaaaa ucaaggauau cuacacacaa gacggguacg     2700

augccuucau gaaaauuuau aagaaggaca aaucuaaguu ucucauguac cgccaugacc     2760

cccagacguu cgaaaaaguc aucgagccua uucuggaaaa cuaucccaau aagcagauca     2820

acgagaaagg aaaggaggug cccuguaauc cuuuucuuaa guacaaagag gaacacggcu     2880

auauuagaaa guacuccaaa aaggggaacg gcccggaaau caaaagccug aaguauuacg     2940

auucuaagcu cggaaaccac auugacauca cccccaaaga uagcaauaac aagguugugc     3000

ugcaguccgu cagcccuugg cgggccgacg uguacuucaa uaaaacaacc ggcaaguaug     3060

agauccuggg gcuuaaauac gcugaucucc aauuugaaaa gggaacaggc accuauaaga     3120

uuucucagga gaaauacaac gacaucaaga aaaaggaggg gguugauucc gacagcgagu     3180

ucaaauuuac gcuguauaag aaugaucugc uccuggugaa agacacagaa accaaggaac     3240

agcaacuuuu ccgcuuucug uccagaacca ugcccaagca gaaacauuac gucgagcuca     3300

agccuuauga uaaacagaag uucgaaggag gcgaggcccu gauuaaagug cuggggaacg     3360

uugcgaauag cggccagugc aagaagggac uuggcaaaag caacaucucc auuuacaagg     3420

ugcggacaga cguccugggg aaucaacaca ucaucaaaaa cgagggagac aagcccaaac     3480

ucgauuucaa gcgcccggcu gccaccaaga aagccggcca ggcuaagaaa aagaaauaag     3540

gacuaguguc caccuguccc uccugggcug cuggauuguc ucguuuuccu gccaaauaaa     3600

caggaucagc gcuuuacaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3660

aaaaaaaaaa aaaaaaaaaa aaaaaaagaa uu                                   3692


<210>  4856
<211>  4250
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4856
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccac ccaguucgag ggauuuacaa accuguacca aguuagcaag acgcuccggu      180

ucgaacugau uccgcagggc aaaacccuua agcauaucca ggagcagggg uuuauugaag      240

aggacaaggc gcgcaaugau cacuauaaag aacugaagcc caucauugac agaaucuaca      300

aaacauaugc ugaucaaugc cuccagcugg ugcagcuuga cugggagaac cuguccgccg      360

ccaucgauuc uuaccggaag gagaaaaccg aagagacacg caaugcucuc auugaagaac      420

aggccaccua uagaaacgcu auccaugacu acuucauugg acggacagau aaucugaccg      480

acgccaucaa caagcgccac gcggagauuu auaagggccu guuuaaagcc gaacucuuca      540

augggaaggu gcugaaacaa cuuggaacgg ucaccacaac cgagcaugaa aacgcucugc      600

ucagauccuu ugauaaguuc acaaccuacu uuagcggcuu cuacgagaau cggaaaaacg      660

uguuuucugc cgaagacauc agcacggcua uuccucaccg caucguucag gauaauuucc      720

ccaaguuuaa agagaacugu cacaucuuca caagacugau uaccgccgug ccuucccuuc      780

gggagcauuu cgaaaauguc aagaaggcga ucggcauuuu ugugucuaca agcaucgagg      840

aaguuuucuc cuuucccuuc uauaaccagc ugcucaccca gacccaaauu gaccuguaca      900

aucagcugcu cgggggaauc agccgcgagg ccggcacaga gaaaaucaag gggcugaacg      960

aggugcuuaa ccuggcuauu cagaaaaaug augagacggc ccacaucauu gcuucucucc     1020

cucauagauu uaucccgcug uucaagcaaa uucuuuccga ccggaacacc cugagcuuua     1080

uccucgaaga guucaaaucu gacgaagagg ucauccaguc cuuuugcaag uauaagacac     1140

ugcugcgcaa ugaaaacgug cucgagaccg ccgaggcccu guucaaugaa cuuaacagca     1200

uugaucugac acacaucuuu auuucucaca aaaagcucga gaccaucucc agcgcucugu     1260

gugaccauug ggauacgcuu agaaaugcgc uguacgaacg gagaauuucc gagcucacag     1320

gaaaaaucac caagucugcc aaagagaagg ugcagcgcag ccugaagcac gaagacauua     1380

accugcagga gaucaucucc gcugccggca aagaacucag cgaggccuuc aagcagaaaa     1440

ccucugaaau ucugucccau gcucacgccg cgcuugauca accccugccu acaacccuca     1500

agaaacagga ggagaaggaa auccuuaaga gccagcugga cucucuucuc gggcuguauc     1560

aucugcucga uugguuugcu gucgacgaga gcaaugaagu ugaucccgag uucuccgccc     1620

ggcugacagg aauuaaacuu gaaauggagc cuucucugag cuucuacaac aaggccagaa     1680

auuaugcuac gaaaaagccc uacuccgugg agaaauuuaa gcucaacuuc caaaugccga     1740

cccuggccag cggcugggac gugaauaaag aaaagaacaa uggcgcuauc cuguuuguca     1800

agaacgggcu uuacuaucuc ggaauuaugc cuaaacagaa gggccgcuac aaagcccugu     1860

cuuucgagcc cacagaaaag accuccgagg gguuugauaa aauguauuac gacuauuucc     1920

cugacgcggc caagaugauc cccaagugca gcacacagcu gaaagcugug accgcccacu     1980

uucagacgca caccacaccc auccuccugu cuaacaauuu cauugaaccu cuugagauca     2040

ccaaggagau uuacgaucug aacaauccgg aaaaagagcc caagaaauuu caaacagcuu     2100

augccaagaa gaccggagac cagaaaggcu accgggaagc gcucuguaag uggaucgauu     2160

ucacgagaga cuuucugucc aaauacacaa agaccacaag cauugaucug uccucucuuc     2220

gcccuagcuc ccaguauaaa gaccucgggg aguacuaugc cgagcugaac ccccugcucu     2280

accauaucuc uuuccagcgg aucgcugaaa aggagauuau ggaugccguu gaaaccggaa     2340

agcuguaucu uuuucaaauc uacaauaaag acuucgcuaa gggccaccau ggcaaaccua     2400

accugcacac ccucuauugg acagggcugu ucagccccga gaaucuggcc aagacgucca     2460

uuaaacuuaa cggacaggcc gaacucuuuu acagaccuaa gagccgcaug aaacggaugg     2520

cucacagacu gggcgagaag augcugaaua agaaacucaa ggaucagaaa accccgaucc     2580

ccgacacacu guaccaagag cuuuaugauu acgugaacca ucgccugucu cacgaccucu     2640

ccgaugaagc gcgggcccug cugccuaaug ucauuaccaa ggaggugagc caugaaauca     2700

ucaaagacag acgguucaca ucugacaagu uuuucuuuca cguucccauu acccuuaacu     2760

aucaggcugc caauuccccu agcaaguuca accagcgcgu gaaugccuac cugaaagagc     2820

aucccgaaac gccgaucauu gggaucgaua gaggagagcg gaaccucauu uauaucacag     2880

ucauugacuc uaccggcaag auccuggagc agagaagccu caacaccauc caacaguuug     2940

auuaccagaa aaagcuggac aaucgcgaaa aagagcgggu ggcugccaga caggcguggu     3000

ccgucguggg gacaauuaag gaucuuaagc aaggcuaucu gagccagguu auccacgaaa     3060

uuguggaccu caugauccac uaccaggcug ucgugguucu ggagaaccug aauuucggau     3120

uuaaaucuaa gcgcaccggc auugccgaaa aagccgugua ccagcaauuc gagaagaugc     3180

uuaucgauaa acugaacugc cucguccuga aggacuaucc ugcugagaag guugggggag     3240

ugcucaaucc cuaccagcug acagaucagu uuacguccuu cgccaaaaug ggcacccaaa     3300

gcggguuccu guuuuaugug cccgcgccuu acacauccaa gaucgacccg cugaccggau     3360

ucgucgaucc cuuugugugg aaaacaauua agaaccauga aucuagaaaa cacuuccucg     3420

agggcuuuga cuuccugcau uaugauguga agaccgggga cuuuauccug cacuucaaga     3480

ugaaucggaa ccuuagcuuu cagcgcggcc ugccuggauu caugcccgcu ugggacauug     3540

ucuuugagaa aaaugaaacg caguucgaug ccaagggcac cccuuuuauc gccgggaaaa     3600

gaauugugcc cguuaucgag aaccaucggu ucacaggacg cuacagagac cucuauccug     3660

cuaaugaacu gaucgcccuc cuggaggaaa agggcauugu guuccgggau ggguccaaca     3720

uccuucccaa acugcuggag aaugacgauu cucacgcuau ugacaccaug gucgcccuca     3780

uccgcagcgu gcugcagaug agaaacucca augcggccac aggagaagau uacauuaacu     3840

cuccgguucg ggaccuuaac ggcguguguu uugauagccg cuuccaaaau ccugaguggc     3900

ccauggacgc ugaugccaac ggggcuuacc acaucgcccu gaagggccag cuccugcuca     3960

aucaucugaa agaguccaag gaccuuaagc ugcagaacgg aauuagcaau caggacuggc     4020

uggccuauau ccaagaacuc agaaacaaac ggccugcugc gaccaagaaa gccggccagg     4080

cuaagaaaaa gaaguaagga cuagugucca ccugucccuc cugggcugcu ggauugucuc     4140

guuuuccugc caaauaaaca ggaucagcgc uuuacagauc uaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaauu                4250


<210>  4857
<211>  4229
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 variant mRNA product

<400>  4857
gggagagucc cgcagucggc guccagcggc ucugcuuguu cgugugugug ucguugcagg       60

ccuuauucaa gcuuaccaug gcccccaaga aaaagagaaa agugggcauc cacggggucc      120

cugcugccag cauuuaccag gaguucguua acaaguauuc ccugucuaaa acccuccggu      180

uugaacugau cccgcaagga aagacacuug agaauauuaa ggcgcgcggc cugauccucg      240

acgaugaaaa gagagcuaag gacuacaaaa aggccaaaca gauuaucgau aaguaucauc      300

aguucuuuau cgaggaaauu cugagcuccg ugugcaucuc ugaggaccuu cugcagaacu      360

acagcgaugu guauuucaag cucaaaaagu ccgacgauga caaucugcaa aaagauuuua      420

agucugccaa agacacgauu aagaaacaga ucagcgagua cauuaaggau uccgaaaagu      480

ucaaaaaccu guuuaaucag aaccucaucg acgcuaagaa agggcaggag agcgaucuga      540

uucuuuggcu gaagcaaucu aaagacaaug gaaucgaacu cuucaaggcc aacuccgaca      600

ucaccgauau ugacgaggcu cuggagauca uuaagagcuu uaaaggcugg acaaccuauu      660

ucaagggguu ucacgaaaau cggaaaaacg ucuacucuuc caaugauauc cccacaagca      720

uuaucuaccg caucguggac gauaaccuuc cuaaguuccu ggagaauaaa gccaaguaug      780

aaucucucaa ggacaaagcg cccgaggcca uuaacuacga acagaucaag aaagaucugg      840

cugaggagcu gaccuuugac auugauuaua agacauccga aguuaaucag agaguguuca      900

gccucgacga ggucuucgaa aucgccaacu uuaacaauua ccugaaccag uccggaauua      960

ccaaauucaa uacgaucauc ggcggcaagu uugugaacgg ggagaauacc aagcggaaag     1020

gaauuaacga auauaucaau cuuuacucuc aacagauuaa cgauaagaca cugaaaaagu     1080

auaaaaugag cguucucuuc aagcagaucc uguccgacac cgagagcaaa ucuuuuguga     1140

uugacaaacu ugaggaugac uccgaugucg ugacaaccau gcagagcuuc uacgaacaaa     1200

ucgcugccuu uaagacggug gaggaaaaau cuauuaagga gacacugagc cuccuguucg     1260

acgaucugaa agcgcagaag cucgaccugu ccaaaaucua cuuuaagaau gauaagucuc     1320

uuaccgaccu gagccagcaa gucuucgaug acuauuccgu uaucggcaca gccgugcucg     1380

aauacauuac ccagcagauc gcuccuaaaa accuggauaa ucccagcaag aaagagcagg     1440

agcuuauugc caagaaaacc gaaaaggcua aguaucuguc ucucgagaca aucaaacugg     1500

cccuggaaga guuuaacaag caucgcgaca uugauaaaca auguagauuc gaggaaaucc     1560

ucgccaauuu ugcugcgauc ccuaugauuu ucgacgagau cgcccagaac aaggacaauc     1620

uggcucagau uuccaucaaa uaccagaacc aagggaagaa ggaucuucug caggccagcg     1680

ccgaagacga ugugaaagcu auuaaggacc uccuggauca gacgaacaau cugcuucaca     1740

aacucaagau cuuccauauc ucucaguccg aggacaaagc caacauucug gauaaggacg     1800

aacacuuuua ucuggucuuc gaggagugcu acuuugaacu cgcgaauauc gugccgcugu     1860

auaacaaaau ucggaauuac aucacccaaa agcccuacag cgaugagaag uucaaacuua     1920

acuuugaaaa uuccacacug gcuaacggau gggacaagaa uaaagagccu gauaacaccg     1980

ccauucucuu caucaaggac gauaaauauu accugggcgu uaugaauaag aagaacaaua     2040

aaaucuuuga cgacaaggcc auuaaagaaa acaaggggga gggauauaaa aagaucgugu     2100

acaagcugcu ucccggcgcu aauaaaaugc ucccuaaggu cuucuuuucu gccaaaagca     2160

uuaaguucua uaaccccucc gaggauaucc ugcgcauuag aaaccacucu acacauacca     2220

aaaauggcag cccgcagaag ggguacgaaa aguuugaguu caacaucgaa gacugucgga     2280

aauuuauuga uuucuauaag caguccauca gcaaacaccc ugaguggaag gacuucggau     2340

uucgcuucuc ugauacgcaa agauacaauu ccaucgacga auuuuaccgg gagguggaga     2400

accagggcua uaaacugaca uucgaaaaua uuagcgaguc uuacaucgau uccguuguga     2460

accaggggaa gcucuaucug uuucagauuu acaauaagga cuucagcgcu uauucuaaag     2520

gacgccccaa ccuucauacc cuguacugga aggcccucuu ugaugaaaga aaucugcaag     2580

acgucgugua uaaacugaac ggcgaggcgg agcuuuucua ccggaagcag agcaucccua     2640

aaaagauuac ccaccccgcc aaagaagcua ucgccaauaa gaacaaggau aaucccaaaa     2700

aggaguccgu cuuugaauac gaccucauca aagacaagag auucacagag gauaaauuuu     2760

ucuuccacug cccuauuacc aucaacuuua agagcucugg ggcuaauaag uucaacgacg     2820

aaauuaaccu gcugcucaaa gagaaggcca augaugugca uauccugucc auugaccgcg     2880

gagagcggca ccuugcguau uacacacugg uugauggcaa aggcaacauc aucaagcagg     2940

acacguuuaa uauuaucggg aacgauagaa ugaaaaccaa uuaucaugac aagcucgccg     3000

cuauugaaaa ggaucgcgac agcgcccgga aagauuggaa gaaaaucaac aauauuaagg     3060

agaugaaaga aggauaccug ucccaggugg uccacgagau cgcuaagcug gugauugaau     3120

auaacgccau cguuguguuc gaggaccuua auuuuggcuu caagagaggg cgcuuuaaag     3180

ucgagaagca aguguaccag aaacucgaaa agaugcugau cgagaaacug aacuaucucg     3240

uguucaagga uaaugaauuu gacaaaacag gaggcguucu gcgggccuac cagcuuaccg     3300

cuccguucga gacauuuaag aagaugggga aacagaccgg cauuaucuac uauguccccg     3360

cgggauucac gucuaagauu uguccuguga ccggcuuugu gaaccaacug uaccccaaau     3420

augaaagcgu cuccaagucu caggaguucu ucagcaaauu ugacaagauc ugcuacaauc     3480

ucgauaaggg guauuucgag uuuuccuucg acuacaaaaa cuuuggagau aaggccgcua     3540

aaggcaagug gacaauugcc ucuuucggga gcagacugau caauuuucgc aacuccgaca     3600

aaaaucauaa cugggauacc cgggaagugu auccuacaaa ggagcuggaa aaacuucuga     3660

aagacuacag caucgaguac ggacacggcg aguguauuaa ggccgcuauc ugcggggaau     3720

cugauaaaaa guucuuugcc aaacucaccu ccguucugaa cacgauucuc cagaugagaa     3780

auagcaagac aggcaccgag cuggacuauc uuaucucucc cguggcggau gucaacggaa     3840

auuucuuuga cucccgccaa gcuccgaaga acaugccuca ggaugccgac gccaauggcg     3900

cuuaccacau ugggcugaaa ggacucaugc ugcugggccg gaucaagaac aaucaggaag     3960

ggaaaaagcu gaaccuugug aucaaaaaug aggaauauuu cgaguuuguu cagaacagaa     4020

auaacaagcg gcccgccgcg acaaagaagg cuggacaagc caagaaaaag aaauaaggac     4080

uaguguccac cugucccucc ugggcugcug gauugucucg uuuuccugcc aaauaaacag     4140

gaucagcgcu uuacagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaagaauu                                       4229


<210>  4858
<211>  51
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS2 variant

<400>  4858
auggcaccaa aaaaaaaaag aaaaguagga auacacggag uaccagcagc a                51


<210>  4859
<211>  48
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NLS4 variant

<400>  4859
aaaagaccag cagcaacaaa aaaagcagga caagcaaaaa aaaaaaaa                    48


<210>  4860
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4860
auggauaaga aauauaguau aggccuugau auaggcacca auagcguagg augggccgua       60

auaacagaug aauauaaagu uccaagcaag aaauuuaaag uauuaggaaa cacagaucga      120

cauucaauua agaaaaaccu aauuggagca cuacuuuucg acaguggaga aacagcagaa      180

gcaacucguc uaaaaagaac cgcaagacga agauauacuc gaagaaagaa cagaauuugc      240

uaccuacaag aaauauuuuc uaacgaaaug gcuaaaguag acgacucauu uuuccacaga      300

cuagaagaau ccuuucuggu ugaagaagau aagaaacaug aacgacaucc gauuuucgga      360

aauauagucg augaaguggc auaccacgaa aaauacccaa ccaucuacca ccuucgaaag      420

aaauuagugg acagcacaga caaagcagac uuaagguuaa uauaucuagc uuuggcacac      480

augaucaaau uccgaggaca uuuucuaauu gagggagauc uaaaccccga caacucugau      540

gucgacaaac uauuuauaca auuaguacaa acuuacaauc aacuauuuga ggaaaaccca      600

aucaacgccu caggugucga cgcaaaagcc auucuaucag caagauuauc aaaaagccga      660

agacuagaaa acuugaucgc acaacuccca ggagagaaga aaaauggacu cuucggaaau      720

cuuauagcgc ucuccuuagg cuuaacaccc aacuucaaau caaauuucga ccuagcagaa      780

gaugcaaaac uacaacuuuc caaagauacu uaugacgacg acuuggacaa cuuacuagcu      840

cagauaggag aucaauaugc ugaucuauuu uuagccgcca aaaaucucuc agacgcaauc      900

uuacuaagug auauucuaag aguaaauaca gaaauaacca aagccccucu auccgcaagu      960

augaucaaaa gauacgacga acaccaccaa gaccugacgc uacuaaaagc ccuaguacgu     1020

caacaauuac cagaaaaaua caaggaaaua uucuuugauc aauccaagaa cggauacgcc     1080

ggauacauag auggaggggc aucacaagaa gaauuuuaca aauuuauaaa accaauucua     1140

gagaaaaugg augggacgga agaacuauua gugaaacuaa accgagaaga cuuacuacgu     1200

aaacaaagaa cguucgauaa uggcucuaua ccacaccaaa uccacuuagg agaacuacac     1260

gccauauuac gacgccaaga agacuuuuau ccauuccuaa aagauaaucg agagaaaaua     1320

gagaaaauuu uaaccuuccg aauaccguau uacguaggcc cucucgcgcg ugguaacuca     1380

agauucgcau ggaugacuag aaaaucagaa gaaacuauca caccauggaa cuucgaagaa     1440

gucguagaca aaggagcgag ugcucaaucu uuuauagaaa gaaugacuaa cuucgauaaa     1500

aauuuaccaa augaaaaagu acuaccaaaa cacagcuugc uauaugaaua cuucacagua     1560

uacaaugaac uuacaaaagu uaaauaugua acagaaggua ugagaaaacc agccuucuua     1620

agcggagaac aaaagaaagc aaucgucgau uuauuauuua aaacaaacag gaaaguaacu     1680

gucaaacaau uaaaagaaga cuacuuuaag aaaauagaau guuucgauuc aguagaaaua     1740

ucaggaguag aagaccgauu caacgcauca cuggggacau accacgaccu acuuaaaaua     1800

aucaaagaca aagauuuccu agacaaugaa gaaaacgaag auauacuuga agauauagua     1860

cuaaccuuaa cccuauuuga agacagagaa augauagaag aaagacuaaa aacuuaugca     1920

caccuauuug augacaaggu gaugaaacaa cucaaaagaa gaagauacac agguugggga     1980

agacuaucaa gaaaauuaau caauggcauc agagacaaac aguccggaaa aacaauacug     2040

gacuuucuga aaucagacgg auucgccaac agaaauuuca ugcaauuaau acacgaugac     2100

agccuaacau ucaaagaaga uauacaaaaa gcucaaguaa gcggccaagg ggacucucua     2160

caugaacaca ucgcuaaccu agcaggauca ccagcaauaa agaaagggau uuuacaaacu     2220

guuaaaguug uagacgagcu aguaaaaguc augggaagac auaaaccgga aaauauaguu     2280

auagaaaugg cacgugaaaa ccaaacuacu caaaaagggc agaaaaacuc aagagaaagg     2340

augaaaagaa uugaagaagg cauaaaagaa uuagggagcc aaauucuaaa agaacaccca     2400

guagaaaaca cacaacuuca aaacgagaaa cuguaucuuu acuauuuaca aaacgguaga     2460

gacauguacg uagaucaaga auuagauauc aaccgccuau cugauuacga ugucgaccau     2520

auagucccac aaucauucuu aaaagaugau ucgauagaca acaagguauu gaccaggagu     2580

gacaaaaaca gggggaaauc agacaaugua ccaucagaag aggugguaaa gaaaaugaaa     2640

aacuauuggc gacaacuuuu aaaugcuaaa uugauaacuc aaagaaaguu ugauaaucuc     2700

acgaaagccg aacguggcgg ccugagcgaa cucgauaaag cagguuucau aaaacgacag     2760

cuugucgaaa cuagacaaau aacaaaacac gucgcacaaa uacuggacuc aagaaugaau     2820

acuaaauacg augaaaacga caaauuaaua agagaaguaa aaguaauaac acuaaaguca     2880

aaauugguau cagauuuuag aaaagauuuc caauucuaca aaguucgcga aauaaauaau     2940

uaucaucacg cacacgacgc uuaucuaaac gcaguaguag gaacugcacu aauaaagaaa     3000

uauccuaaau uagaaucaga auuuguauac ggcgacuaua aaguguacga uguacgaaaa     3060

augaucgcaa aaucagaaca ggaaauaggc aaagcaaccg ccaaauacuu uuucuauucu     3120

aauaucauga acuucuuuaa aacagaaauc acacuugcaa acggggaaau acguaaaaga     3180

ccccuuauag aaacuaacgg ggaaacagga gaaauaguau gggacaaagg cagagacuuu     3240

gcaacaguaa gaaaaguacu uucaaugccc caaguaaaca uaguaaagaa aaccgaagua     3300

caaacgggcg gcuuuucaaa agaaucaauu uuaccuaaac gaaacagcga uaaacuaaua     3360

gcuagaaaga aagauuggga uccaaagaaa uauggaggau uugauagccc caccguagca     3420

uauuccgucc uuguaguugc aaaaguagaa aaaggaaaaa guaagaaauu aaaaucgguc     3480

aaagaauuau uaggaauuac cauaauggaa agaaguucgu uugagaaaaa cccaauugac     3540

uuccuggaag caaaaggcua uaaagaaguc aagaaagauc ucauuaucaa acuuccaaaa     3600

uauagucuau uugaacuaga gaauggaaga aaacgcaugc uagcgagcgc uggugaacua     3660

cagaaaggaa acgaacuagc auuacccagc aaauacguua auuuccuaua ucuugcaagc     3720

cauuaugaaa aacuaaaagg cuccccagaa gauaaugaac aaaaacaacu auuuguagaa     3780

caacauaaac auuaucuaga ugaaauuaua gaacaaauaa gcgaguucuc caaaagggua     3840

auacuagcag augcaaauuu ggacaaagua cuuucggcau auaauaaaca cagggauaaa     3900

ccaaucagag aacaagccga aaauaucaua cacuuauuua cauuaaccaa uuuaggagcc     3960

ccagcggcau uuaaauauuu ugauacaacc auugacagaa aaagauauac aucaacuaaa     4020

gaagugcuug acgcaacccu aauacaucaa ucgauaacag gauuauacga aacgcgcauu     4080

gacuuaaguc aacuaggcgg ugacuga                                         4107


<210>  4861
<211>  4890
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4861
augaauuuca aaauacuucc aauagcgauu gauuuaggug uuaaaaacac cgguguauuu       60

agcgcguucu accaaaaagg aacgucacuu gaaaggcugg auaauaaaaa ugguaagguc      120

uaugaacugu caaaagauag uuacacgcuc cuuaugaaua accgaacugc acgaagacac      180

caacgaagag gcauugauag aaaacaacuc gucaaaaggu uauuuaaacu cauauggacu      240

gaacaacuga accuagaaug ggacaaagac acccaacaag ccaucaguuu ccuuuuuaac      300

agacgaggcu uuucauuuau aaccgaugga uauuccccug aauaccucaa cauaguacca      360

gagcaaguaa aagcaaucuu aauggauaua uuugacgacu auaauggcga agaugaucuc      420

gacucuuauc ugaagcucgc cacugaacaa gaauccaaaa uuucagaaau cuacaacaaa      480

uuaaugcaaa aaauauuaga auuuaaacuc augaaacuau guacggacau aaaagacgac      540

aaagucucaa caaaaacacu gaaagaaauu acgagcuaug aauucgaacu auuagcagac      600

uaucuagcaa acuauucuga aucacucaaa acacaaaaau ucucauacac cgacaaacaa      660

ggaaauuuaa aagaacucuc auacuaccau cacgauaaau acaauaucca agaguuccua      720

aaacgacacg caacuaucaa ugauagaaua cucgauacac uacucacuga cgaucuggau      780

auuuggaacu ucaacuucga aaaauucgac uuugacaaga augaagaaaa acuacaaaac      840

caagaagaua aagaccauau ccaagcacau cuacaccauu ucguauuugc aguaaacaaa      900

auaaaaucug agauggcauc agguggacgu cacagaucac aauacuuuca agaaaucacu      960

aauguacuag acgaaaauaa ccaccaagaa ggguacuuga aaaauuucug ugagaaccua     1020

cacaacaaaa aauacagcaa uuuaaguguu aaaaaucuag ugaaccuaau cggcaaccua     1080

aguaacuugg aauuaaaacc ccucaggaaa uauuucaacg auaaaauaca cgcaaaagcg     1140

gaccacuggg acgaacaaaa auucacagaa acauacugcc acuggauacu cggcgaaugg     1200

agagugggag uuaaagauca ggauaaaaaa gacggugcaa aauacucaua caaagaucua     1260

ugcaacgagc uaaaacagaa aguaaccaag gcgggacuag uagauuucuu acucgaauua     1320

gacccaugca gaacaauucc accauaccua gacaacaaca auaggaaacc cccgaaaugc     1380

caaucauuaa ucuuaaaucc caaauuucua gacaaccaau auccuaauug gcaacaguac     1440

uuacaagagc uaaaaaaauu acaaucaaua caaaauuacu uggauucuuu cgaaaccgau     1500

cuaaaaguac uuaaaaguag uaaggaccaa ccauacuucg uagaauauaa auccaguaau     1560

caacaaauag cgagcggaca acgagacuac aaggaucuug acgccagaau uuuacaauuu     1620

auauuugauc gaguaaaagc uucugacgaa uuauuacuaa acgaaauuua cuuccaggca     1680

aaaaaacuga agcaaaaagc auccucagag cucgagaaau uggaaucuuc aaaaaaacua     1740

gaugagguaa uagcgaauuc acaacuuucc caaauacuaa aaagucaaca uaccaacgga     1800

auauucgaac aggggacauu ccuccaucuc guguguaaau auuauaagca gagacaaaga     1860

gcucgagauu cacgucuaua uaucaugccu gaguaccgau acgauaaaaa auuacauaaa     1920

uacaacaaua cugguagauu cgacgaugau aaucaacuac uaaccuauug caaucauaaa     1980

ccaagacaga aacgauacca acucuuaaac gauuuagcgg gaguacucca aguauccccu     2040

aauuuucuaa aagauaaaau cggaagugau gacgaccucu ucaucucgaa auggcuggua     2100

gaacacauac gcggcuucaa gaaagcaugu gaagacuccc uaaaaauaca aaaagacaau     2160

agaggccuac uuaaccauaa gaucaauauc gcacguaaca ccaaaggcaa augcgaaaaa     2220

gaaauauuua aucuaauuug caaaauagaa ggcucagaag auaaaaaagg uaauuacaaa     2280

cauggcuuag cauacgaacu ggggguuuua cuauuuggag aacccaacga ggcaucaaaa     2340

ccagaauuug acagaaaaau caaaaaauuc aauucaauuu auagcuucgc ccaaauucaa     2400

cagauagccu uugccgaaag aaagggaaac gcaaacaccu gcgccguaug uagugcagau     2460

aacgcucacc gcaugcaaca aauuaagauc acggaaccag uagaagauaa caaagacaaa     2520

aucaucuuaa gcgcaaaagc acaacgccua ccggcaauac caaccagaau cguagaugga     2580

gcggugaaaa aaauggcaac cauacuugca aaaaauauag ucgacgacaa uuggcaaaac     2640

auuaaacaag uguuaagcgc caaacaccaa cuucacauac ccaucaucac ggagaguaac     2700

gcauuugaau ucgaaccggc gcucgccgac guaaaaggua aaucucuaaa agauagaagg     2760

aaaaaagccc uagaaagaau uagcccagag aauaucuuua aagacaaaaa caauagaaua     2820

aaagaauucg ccaaaggaau aagcgcguac ucaggagcaa auuuaacaga cggcgauuuu     2880

gacggagcua aggaagaacu agaccacauu auacccagaa gccacaaaaa auauggaacc     2940

cugaacgaug aagcaaauuu aauaugcgua acacgaggag auaacaaaaa caaagguaau     3000

agaauauucu gucucagaga uuuagccgac aauuacaaau uaaaacaguu cgaaaccacc     3060

gacgaccuag aaauagaaaa aaaaaucgcu gauaccauau gggaugcaaa caaaaaagau     3120

uuuaaauuug guaacuaccg cuccuuuauc aaccuaacuc cacaagaaca aaaagcauuc     3180

aggcaugccc uauuccuagc agacgaaaac ccuauaaaac aagcuguuau acgagcuaua     3240

aacaacagaa accgaacauu uguuaaugga acacaaagau acuuugcaga aguccuagca     3300

aacaacauuu accuaagagc gaaaaaggaa aauuuaaaca cagauaaaau aaguuucgac     3360

uacuuuggaa ucccaacuau cggaaaugga agaggcauag ccgaaauucg acaacuguac     3420

gaaaaagucg acucagauau ccaagcauac gcuaaaggcg auaagccaca ggcaucauau     3480

ucucaccuaa uugaugcaau gcuugcauuc uguaucgcug cagacgaaca cagaaaugau     3540

ggaucaauag gacuagaaau agauaaaaac uauucacuau acccgcuaga caaaaacacc     3600

ggagaaguuu ucacuaaaga caucuuuuca caaauuaaga uuacagauaa ugaauuuucc     3660

gacaaaaaau uaguaagaaa aaaagcuauc gaaggguuca acacucauag acagaugacu     3720

cgagacggaa ucuacgcaga aaauuaccua ccuauauuaa uacauaagga auuaaaugaa     3780

gucagaaagg gauacacuug gaaaaauuca gaagaaauaa aaaucuuuaa aggaaaaaaa     3840

uaugauauac aacaacuaaa caaccuuguu uauuguuuaa aauucgucga uaaaccaaua     3900

agcauagaua uacaaauauc cacccuagaa gaauuaagaa acauucuaac cacaaacaau     3960

auagcugcga cggcugaaua cuauuauauc aaccuaaaga cacaaaaacu ccacgaguac     4020

uacauagaga acuauaacac agcccuaggu uauaaaaaau acagcaaaga aauggaauuu     4080

cugcgaucuc uagcauaccg cucggaacgu guuaaaauua aauccauuga cgacguaaaa     4140

cagguacucg acaaagauuc aaauuuuaua auaggaaaga uaacuuuacc auucaaaaaa     4200

gaguggcaac gauuauaccg agaauggcaa aauaccacaa uaaaagauga uuacgaauuc     4260

uuaaaauccu uuuucaacgu caaguccauc acuaaacucc acaaaaaagu gcgaaaagac     4320

uuuaguuuac caauaucgac uaacgaaggc aaauuuuuag uaaaaagaaa aacaugggau     4380

aacaacuuca ucuaccaaau ccuuaacgac ucagacagua gagcggaugg uacaaaaccc     4440

uuuauuccug ccuucgauau uaguaaaaac gaaauugucg aagcaauaau agauaguuuc     4500

acuucgaaaa acauauuuug guuaccaaaa aacauagaac uacaaaaagu cgacaacaaa     4560

aauauuuucg ccaucgauac uagcaaaugg uucgaaguag aaacaccauc ggacuuaaga     4620

gacaucggaa ucgcaacuau ccaauacaaa auagauaaca acucacgucc aaagguaaga     4680

guaaaacuag auuauguaau agacgacgac aguaagauaa auuacuucau gaaccauucu     4740

cuuuuaaaau caagauaucc cgauaaggua uuggaaaucc uaaaacaauc aacaauaaua     4800

gaauuugaaa guagcggauu uaacaaaaca aucaaagaaa uguuagguau gaaacuagcc     4860

gggauuuaua acgagacauc aaauaauuga                                      4890


<210>  4862
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4862
augaaacgaa acuacauccu aggacuagau auaggcauaa ccucuguagg guacggcauc       60

auagacuacg aaacaaggga cguaauugau gcaggugucc gauuauuuaa agaagcaaac      120

guagaaaaua acgaaggcag aagaucaaaa agaggugcca gaagacucaa acgaaggcga      180

cgacaccgga uacaaagagu caaaaaauua cucuucgauu auaauuuauu aacagaucac      240

uccgaacugu ccggcauaaa uccauaugaa gcaagaguca aaggacuguc ccaaaaacua      300

ucagaagaag aauucucagc ugcacuacua caccucgcca aacgacgcgg uguacauaac      360

guaaacgaag uagaagaaga cacaggaaac gaacuaucca cgaaagaaca aaucucacgu      420

aacaguaaag cuuuggaaga aaaauacguc gcagagcuuc aacuagagag acugaaaaaa      480

gacggggaag ugaggggauc aauaaauaga uucaaaacca gcgauuacgu uaaggaagca      540

aaacaacuac uaaaaguuca aaaggccuac caccaacuag aucaaucauu cauagauaca      600

uacauugacc uacuagaaac acguagaaca uacuacgagg gucccggaga aggaucuccg      660

uucggaugga aggacauuaa agaaugguac gaaaugcuaa ugggacauug uacauauuuu      720

ccagaagaau uacgcucugu aaaauaugcu uacaacgccg acuuauacaa cgcuuuaaau      780

gacuuaaaua acuuaguaau aacaagagau gagaacgaaa aacuagaaua uuaugaaaaa      840

uuccaaauca uugaaaaugu auucaagcaa aagaaaaaac cuacauugaa acagauagcc      900

aaagaaauuc ucguaaacga agaagauauc aaaggcuaca gaguaacauc uaccggcaaa      960

cccgaauuca ccaaucuuaa aguauaccac gauauaaaag acauaacagc cagaaaagaa     1020

auaaucgaaa acgcagaauu acuagaucaa auagcgaaaa uacuaaccau uuaccagucc     1080

uccgaggaca uacaagaaga auuaacaaac cuaaacucag aauuaacaca agaagagaua     1140

gaacaaauau cuaaccucaa aggauacaca ggaacucaua acuuaagcuu aaaagcaauc     1200

aaccuaauac uugacgaacu uuggcacaca aacgacaauc aaauagcaau cuuuaacagg     1260

cuaaaacuag uaccaaaaaa aguagacuua ucgcaacaaa aagaaauacc cacaacacua     1320

gucgaugacu ucauucuauc acccguagua aaaagaucau ucauacaaag cauuaaaguu     1380

aucaaugcca uaaucaaaaa auacggauug ccuaaugaua uaaucaucga acuagccaga     1440

gaaaaaaaca gcaaagaugc acaaaaaaug auaaacgaaa ugcaaaaacg aaauagacag     1500

acgaaugaac gaauagaaga aaucauacgu acaacaggua aagaaaaugc caaauaccua     1560

auagaaaaaa ucaaauuaca cgacaugcaa gaaggaaaau gccucuauuc ccuugaagcu     1620

auaccauuag aagacuuacu aaauaacccc uucaauuacg aaguugauca cauaauacca     1680

agaaguguau cauucgacaa uucauuuaac aauaaaguuc ucguaaaaca agaagaaaac     1740

agcaaaaaag guaauagaac uccauuccaa uauuuaucca guagcgacuc caaaauauca     1800

uacgaaacuu uuaaaaaaca cauauuaaau uuagcaaagg gaaaaggaag aauaagcaaa     1860

acaaaaaaag aauacuuauu agaagaaaga gacaucaaca gauuuucagu ccaaaaagac     1920

uuuauaaaua gaaacuuagu agauacccgc uaugcaacuc ggggucucau gaaccuuuua     1980

agaucauauu ucaggguaaa caacuuagau gucaaaguaa aaucaauuaa cggaggauuu     2040

acuucuuucu uaagaagaaa auggaaauuc aaaaaagaaa ggaacaaagg uuauaaacau     2100

cacgccgaag acgcacuaau aauagcaaac gcagauuuca uauucaaaga auggaaaaaa     2160

uuagacaaag caaagaaagu aauggaaaac caaauguuug aagaaaaaca agcagaauca     2220

augccagaga ucgaaacaga gcaagaauac aaagaaauau ucaucacccc ccaucaaaua     2280

aagcacauua aagacuucaa agacuacaaa uacucacaua gaguagacaa aaaaccaaau     2340

agagaacuca ucaaugacac auuauacucg acacgaaaag acgacaaagg aaacacccuc     2400

auuguaaaua aucuaaacgg ccuauacgac aaagacaacg acaaacuaaa aaaacucaua     2460

aacaaaucac ccgaaaaauu acucauguac caccacgacc cacaaacaua ccaaaaacuc     2520

aaacugauaa uggaacaaua cggagacgaa aaaaaucccc uuuacaaaua cuacgaagaa     2580

acaggaaacu acuuaacaaa auauaguaag aaagacaaug gacccguaau aaaaaaaaua     2640

aaauauuaug gcaauaaacu gaacgcacau uuagacauaa cagaugacua cccaaauagu     2700

agaaacaaag uugucaaacu aagcuuaaaa ccauacagau ucgauguaua ccuggacaac     2760

ggcguauaca aauucguaac cgugaaaaac cuugacguca uaaaaaaaga aaauuacuau     2820

gaagugaacu caaaauguua ugaagaagca aaaaaauuaa aaaaaauuuc gaaucaagcg     2880

gaauucauag ccuccuuuua caacaacgac uuaauaaaaa uaaacggaga acucuaucga     2940

guaaucggag uaaauaauga ccuccuaaac agaauugagg uaaauaugau agacauuacu     3000

uacagagaau aucuagaaaa uaugaacgac aaacgcccac cacgaauaau aaaaacaaua     3060

gcaagcaaaa cacaaagcau aaaaaaauac uccacugaca uccuuggaaa ucuauaugaa     3120

guaaaaucua aaaaacaucc acaaauaaua aagaagggau ga                        3162


<210>  4863
<211>  4230
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4863
augcuauuua auaaaugcau aauaaucagu auaaaccuug auuucucuaa uaaagaaaaa       60

uguaugacga aaccauauuc aauaggcuua gacaucggga caaauagcgu aggaugggcg      120

guaauaacag auaauuauaa aguaccgucg aagaaaauga aaguauuggg aaacacaucc      180

aaaaaauaca ucaagaaaaa ccucuuaggc guuuuacucu ucgacuccgg aauuacggca      240

gaaggcagac gacuaaagag aaccgcaaga agacguuaua cacgaagaag aaaucgaaua      300

cuuuaucuac aagaaauauu caguacagaa auggcaacac uugacgaugc auuuuuccag      360

cgccuugacg auaguuuccu cguuccagau gacaaacgag auagcaaaua uccaaucuuu      420

gguaauuuag uagaagaaaa aguuuaucau gaugaguucc caaccauaua ucacuuaaga      480

aaauaccuag cugauaguac uaagaaagca gaucuacguc ucguauaccu agcacuugca      540

cauaugauca aguauagagg acacuucuug aucgaaggag aauuuaauuc caaaaacaac      600

gauauucaaa aaaacuucca agauuuccua gacaccuaua acgcaauuuu cgaaucagac      660

cugagccuag aaaacucaaa acaauuagaa gagauaguga aagauaaaau cucaaaauug      720

gaaaaaaaag accgaauacu aaaacuauuc ccaggagaaa agaacucagg aauauucucc      780

gaauuucuua aacuuauagu gggaaaucaa gcggauuuuc gaaaaugcuu uaacuuagac      840

gaaaaagcaa gucuacacuu uucgaaagaa ucauaugacg aagaccuaga gacauuacua      900

gguuauauag gugaugacua uuccgacgua uucuugaaag caaaaaaauu auaugaugca      960

auuuuauuaa guggguuccu gaccguaaca gauaaugaaa cagaagcacc acuuaguuca     1020

gccaugauua aaagauacaa cgagcacaag gaagaucuag cccuacuaaa agaauacauc     1080

agaaacauau ccuuaaaaac uuauaaugaa guauucaaag augauaccaa aaacggauau     1140

gccggcuaca ucgauggcaa aacgaaccaa gaagacuucu acguuuauuu aaaaaaccua     1200

uuagcggaau uugaaggagc cgacuacuuu cuagaaaaaa uagauagaga ggauuucuua     1260

agaaagcaac gcacauucga uaaugggagu aucccauacc aaauucauuu acaagaaaug     1320

agggcaauac uagacaaaca agcuaaauuc uacccauuuu uggcuaagaa uaaagaacgu     1380

auagaaaaaa uacuuacauu caggauacca uacuauguag gcccauuagc cagaggaaau     1440

agugauuucg ccuggucaau aagaaaaagg aacgaaaaaa ucacaccaug gaacuuugaa     1500

gaugucauug auaaagaauc aagugccgag gcauucauca auagaaugac aucauuugau     1560

cuauaucuuc ccgaagaaaa aguacuaccc aaacauaguc uauuauauga aacuuuuaau     1620

guauacaacg aacuaacuaa aguuagauuc aucgcagaau caaugagaga uuaucaauuc     1680

cuagacucaa aacaaaaaaa agauaucguc agauuauacu uuaaagacaa aagaaaaguu     1740

acagacaaag acaucaucga auaccuucac gcaauuuacg gauacgaugg aauagaacuu     1800

aaaggaauag aaaaacaguu uaacucaagu cuuaguaccu aucaugauuu auuaaacauc     1860

auaaaugaca aagaauuccu agacgacuca ucaaacgaag caauaauaga ggaaauaauc     1920

cacacacuaa ccauauuuga agacagagaa augauaaaac aacggcuauc uaaauuugaa     1980

aauaucuuug acaaauccgu acuaaaaaaa cuaucacgaa ggcauuauac aggauggggc     2040

aaacuguccg cuaaacuuau aaacggaaua agagaugaga aaucaggaaa cacaauauua     2100

gacuaucuaa uugacgaugg aauuaguaau cgaaauuuca ugcaauuaau acacgacgac     2160

gcccuaaguu ucaaaaaaaa aauacaaaag gcacaaauaa uaggugauga agacaaagga     2220

aauauuaaag aagucguuaa gucccuaccu ggaagucccg caaucaaaaa aggaauucuu     2280

caaagcauca aaauaguuga cgaacucgua aaaguuaugg gaggacgaaa acccgaguca     2340

aucguggucg aaauggcuag agaaaaucaa uacacaaauc aaggaaaaag uaacucacaa     2400

caacgauuaa aaagguuaga aaaaucgcuc aaagaauuag gaucaaaaau cuuaaaagaa     2460

aauauaccag ccaaacucuc aaaaauagau aauaacgccu uacagaacga cagauuauau     2520

cuauacuacc ugcaaaacgg aaaagacaug uauacuggag augaccuaga cauugacaga     2580

uuaucuaacu augacauuga ccacaucaua ccacaagcau ucuuaaaaga caauucaauc     2640

gacaacaaag uacucguuag uucagccagc aaucgaggaa aaucggacga uuucccaagc     2700

uuagagguag uuaaaaaaag aaaaacguuc ugguaucaac uauuaaaauc caaauuaauu     2760

agccaacgca aauucgacaa ccugacaaaa gcagaaaggg gcggacuacu uccagaagac     2820

aaggcaggcu uuauacaaag acagcuugua gaaacaaggc aaaucacaaa gcacguggca     2880

agacuucuag acgaaaaauu uaacaauaaa aaagacgaga auaauagagc agucagaacu     2940

guaaaaauaa ucacccucaa auccacauug guaagccagu uucgaaaaga uuuugagcuc     3000

uauaaaguaa gagaaauaaa cgacuuccac cacgcacacg acgccuauuu aaaugccguc     3060

auagccuccg cauuacuuaa gaaauaccca aaauuagaac cagaguuugu auauggagac     3120

uacccaaaau acaauucauu uagagaacga aagucagcga cugagaaagu cuauuuuuac     3180

ucaaacauua ugaacauauu caaaaaaucc auaucucuug cagauggaag aguuaucgaa     3240

cgcccccuaa uagaaguaaa cgaggaaaca ggagaauccg ucuggaacaa agaauccgau     3300

uuagcaaccg uaagaagagu guugagcuac ccccaaguua auguaguaaa aaagguagaa     3360

gaacaaaauc acggacucga cagaggcaaa cccaaaggac uauucaacgc aaaucugagu     3420

aguaagccaa aaccaaacuc uaaugagaac cucguaggag cuaaagagua ucucgaccca     3480

aaaaaauacg guggauaugc cgguauuagc aacaguuuug cuguuuuagu aaaagggacu     3540

auagaaaagg gagcaaaaaa aaaaaucacu aacguauuag aauuucaagg uauaucaaua     3600

uuagacagga uaaacuaccg aaaggacaaa uuaaacuucc ugcuagaaaa aggauacaaa     3660

gauauugaac uuauaauaga acuaccaaaa uauagucugu uugaauuauc agacggcuca     3720

cgacgcaugc ucgcaagcau auuaucaacc aacaauaaac gaggugaaau ccauaaaggc     3780

aaccaaaucu uccuguccca aaaguuugua aaacuucucu accaugcgaa aagaauauca     3840

aacacaauaa acgaaaauca uagaaaauac guagaaaauc acaaaaaaga auuugaagaa     3900

cuuuucuauu auauacucga auuuaacgag aacuauguag gugcaaaaaa aaacggaaaa     3960

uuacuaaaca gcgcuuucca aagcuggcaa aaccacucaa ucgaugaacu augcuccucc     4020

uuuauaggac cuacagguuc agaaagaaag ggucuuuucg aauuaaccuc ccgugguucg     4080

gcagcagauu uugaauuccu uggaguuaaa auaccacgcu accgugauua uacuccauca     4140

agccuucuua aagacgcaac acucauacau caauccguua caggauuaua cgaaacacga     4200

auagaccuag caaaacuagg ggaaggauga                                      4230


<210>  4864
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4864
augugguaug ccucauuaau guccgcacau cauuugagag uggguauuga uguagguacu       60

cacaguguug gacuagcaac acugagagua gaugaccaug gaacaccaau agaauuacuc      120

ucagcuuuau cgcauauaca cgacucagga guagguaagg aaggcaagaa agaccaugac      180

acaaggaaga aacuaagugg gauagcaaga cgagcaagac gucuauugca ccacagaaga      240

acccaacucc agcaacucga ugaaguauua agagacuugg gauuuccaau accuacacca      300

ggugaauuuu uagaucuaaa ugaacaaaca gauccuuaca gaguauggag aguaagagca      360

cgauuagucg aagaaaaacu accugaagaa cucagaggcc cggcuauauc aauggcuguu      420

agacauauug caagacacag aggauggaga aauccauauu cgaaaguuga aucauuacuu      480

aguccugcug aagaaagccc uuuuaugaaa gcauuaagag agagaauauu agcaacuacc      540

ggagaaguau uagaugacgg aauaacgccc ggucaagcaa uggcccaggu agcauuaacu      600

cacaauauaa guaugagagg uccugaaggu auauuaggua aauuacacca aagugacaau      660

gccaacgaaa uacgaaaaau augugcaaga caaggaguau ccccugacgu cugcaaacaa      720

cuauugcgag caguauucaa agcagauucu ccccgaggau cagcagucuc aagaguugcc      780

ccggacccau uacccggaca aggaucauuu agacgagccc caaaauguga cccagaauuu      840

caaagauuua gaauuauaag uauaguagca aacuuaagaa uaucggaaac gaaaggagaa      900

aauagaccuc uuaccgcuga ugaaaggagg cauguaguua cuuucuugac agaagauucu      960

caagcggacc ucacaugggu ugauguagcu gaaaaauuag gaguacacag aagagaccuu     1020

cgaggaacag caguacauac agaugacgga gagagaucug cagccagacc accaauagau     1080

gccacggaca gaauaaugag acaaacaaaa auaucaucac uuaaaacaug gugggaagaa     1140

gcagacagug aacaaagagg agcaaugaua agauauuuau acgaagaucc gacagauagu     1200

gagugugcug aaauaauagc agaacuacca gaggaagauc aggccaaauu agauuccuug     1260

caucuaccug cggguagagc ugcauacuca agagaaaguu ugacugcucu aucagaucac     1320

auguuagcua cuacagauga ccuccacgaa gcaagaaaac gacuauuugg agucgaugac     1380

agcugggcac cacccgcuga agcaauaaau gcacccguag guaauccauc gguagauagg     1440

acuuuaaaaa uaguuggacg auaccuauca gcaguugaaa gcaugugggg aacuccugag     1500

guuauccaug uagaacacgu acgagaugga uuuacaagug aaagaauggc cgaugaaaga     1560

gacaaggcaa aucgacgaag guacaacgac aaccaggagg cgaugaagaa aauucaacga     1620

gauuacggaa aagaaggaua caucagccga ggagacauag uaagacuuga cgcuuuagaa     1680

uuacaagguu gugcaugucu auauuguggu acuacaauug gauaucauac augucaauua     1740

gaucacauug uuccacaagc aggcccagga ucaaauaaua gaagaggcaa uuuaguagca     1800

guaugcgaaa gauguaauag gucuaaauca aacacaccuu uugcaguuug ggcacaaaaa     1860

uguggaauac cacacguagg gguuaaggaa gcaauaggaa gaguaagagg uuggaggaaa     1920

caaacuccaa auacuucaag ugaggauuua acgagauuaa agaaagaagu aauugcuaga     1980

uuaagacgaa cacaagaaga uccagaaaua gaugaacgua gcauggaauc gguagcuugg     2040

auggcgaaug aauuacacca uaggauagcg gcagcauacc cagaaacaac uguuaugguu     2100

uacagaggaa guauuacagc cgcagcuaga aaagcagccg gaauagauuc caggauaaau     2160

cuaauaggag agaaggguag aaaagauaga auugaucgaa ggcaucaugc cguggacgcu     2220

ucaguaguug cauuaaugga ggcuagcgua gcaaaaacuc uagcggagcg aucaucacua     2280

cgcggugaac aaagacugac gggaaaagaa caaacaugga aacaauauac aggcaguacu     2340

guaggugcuc gagaacacuu ugaaaugugg agaggacaca ugcuucauuu aacagaacua     2400

uuuaacgaac gucuagcaga agauaaggua uacgucacac aaaauauaag acuacgcuua     2460

agugauggaa acgcucauac gguaaauccc aguaaauuag uaucacauag auuaggagau     2520

ggguuaacag uacaacaaau agauagagcg uguacaccag cauuguggug ugcgcuuacg     2580

agagaaaaag acuuugacga gaaaaacgga cuaccagcgc gagaagauag agcuauacgc     2640

guccacggac acgaaauaaa gucaagcgau uacauacaag uuuucaguaa aaggaagaaa     2700

accgauucag auagagauga aacaccauuc ggagccauag caguucgugg aggguuuguu     2760

gaaauaggac caagcauuca ucacgcuagg auauaucgag uugagggaaa gaaaccagua     2820

uaugcaaugc uaagaguauu uacccaugac cuacugucac aaaggcacgg agauuuauuu     2880

uccgcaguga uaccaccuca aucaauaagc augagaugug cagaaccaaa acuuagaaaa     2940

gccauaacua cgggaaaugc aacauacuua ggcugggucg ugguugguga cgaauuggaa     3000

aucaauguug acaguuuuac gaaauaugcc auuggccgau uuuuagaaga uuucccaaau     3060

acuacaagau ggcgaauuug cggguaugau acaaauucua aauuaacgcu aaaaccuaua     3120

guucucgcag cugaaggguu agagaaccca uccucagcag ucaaugaaau uguagaacuu     3180

aaaggaugga ggguugcaau uaauguauua acaaagguac auccuacagu aguaagaaga     3240

gaugcccucg gacgaccucg cuauucuagc agaucaaacc uacccacauc guggacaaua     3300

gaauga                                                                3306


<210>  4865
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4865
augacuaaac cauauagcau cggauuggac auaggcacaa acaguguagg augggcagua       60

acaaccgaca auuacaaagu uccaagcaaa aaaaugaaag ucuuaggcaa cacuucaaaa      120

aaguacauaa aaaaaaauuu acuaggcgua uuacucuucg auucggguau aacagcugaa      180

ggaagacgac uaaaaagaac agcacgaaga agauacacgc guagaagaaa ccgaauauua      240

uauuuacaag aaaucuucuc aacagaaaug gcaacacuag augacgccuu uuuccaacgg      300

cuagacgacu cuuuucuagu accugacgau aagcgagacu ccaaauaucc uauauucggc      360

aauuuaguag aagagaaggc auaccaugac gaauuuccua cuaucuauca cuuaagaaaa      420

uaucuagcgg acucgacaaa aaaagccgac uuacgauuag uuuaccuagc uuuagcacac      480

augaucaaau acagagguca uuuucuuaua gaaggugaau uuaacagcaa aaauaaugac      540

auacagaaaa acuuccaaga uuuccuugau accuauaaug caaucuucga aagcgaccuu      600

aguuuagaaa auucuaagca auuagaagaa auuguaaaag auaaaauuuc uaaacucgaa      660

aaaaaagaua gaauacuaaa acuuuucccg ggagaaaaaa acucaggaau auucucggaa      720

uucuuaaaau ugaucguagg caaucaagcc gauuucagaa aguguuucaa ucucgacgag      780

aaagcgagcu uacauuucag uaaagaaucu uacgacgaag aucuugagac ccuucuagga      840

uauaucggug augacuacuc cgacguauuu cuuaaagcaa aaaaguuaua cgaugcuaua      900

cuucuaucag guuuucuaac aguaacugau aaugaaacag aagcuccacu uucaagugcc      960

augauaaaaa gauauaacga acauaaagaa gaccucgcuu uacucaaaga auauauccgc     1020

aacauaucac ugaaaacaua caacgaagug uuuaaagacg auacuaaaaa cgguuacgca     1080

gguuauauag auggaaaaac uaaucaagaa gacuuuuacg uguauuuaaa aaaauuauua     1140

gcagaguucg aaggugcuga uuacuuccuu gaaaaaauag acagagaaga cuuuuuaaga     1200

aaacagcgaa cguucgauaa cggauccauc ccauaccaaa uccaccugca agaaaugcgu     1260

gcaauucugg auaaacaagc caaauuuuau ccuuuccuag cuaaaaacaa ggaacgcaua     1320

gaaaaaaucc uuacuuuccg aaucccauac uacguaggcc cauuggcaag aggcaacagc     1380

gacuuugccu ggucaauucg aaaacgcaac gaaaaaauua caccauggaa uuuugaggau     1440

guaaucgaua aagaaucauc agcagaagcc uuuaucaaua ggaugacauc cuuugaucuu     1500

uaucugccag aagaaaaagu acuaccaaag cauucauuac uguacgaaac auuuaacguu     1560

uacaaugaac ugacaaaagu aagauucaua gcagaaucga ugagagacua ccaguucuua     1620

gauuccaaac aaaaaaagga uauaguucga cuuuacuuua aagauaaaag aaaagucaca     1680

gauaaagaua uaauagagua cuuacacgcc auauauggau augauggaau agagcuaaaa     1740

gguauagaaa aacaauucaa cagcucacua uccacauauc augacuuacu aaauauaaua     1800

aacgauaaag aauucuuaga cgacagcucu aaugaagcca uaauagagga gauaauucac     1860

acacuuacua uauuugaaga cagggaaaug auaaaacaaa gacuguccaa auucgaaaau     1920

aucuucgaua aaucaguauu aaaaaaacuu ucucgacgac acuacacagg uuggggaaaa     1980

cuauccgcca aauuaauaaa cggaauaaga gaugaaaaga guggaaacac aauacuugac     2040

uacuugauag augauggaau cucaaauagg aauuucaugc aauuaauaca ugaugaugca     2100

uuaucauuca aaaaaaaaau acaaaaagcg caaauuauag gugacgaaga uaaaggaaac     2160

auaaaagaag uuguuaaaag ccuuccaggc ucaccagcaa ucaagaaagg aauccuucaa     2220

ucaaucaaaa uagucgauga auuaguuaag gucaugggag guagaaaacc agaaucgaua     2280

gucguggaaa uggcacgaga aaaucaauau acaaaucaag gcaaaucuaa cagccagcag     2340

agacuaaaaa gacuugaaaa aagucucaaa gaacuaggca gcaaaauacu aaaagaaaac     2400

auacccgcaa aacuaucgaa aauagacaac aacgcacuuc aaaacgaucg cuuguaucua     2460

uacuauuuac aaaacgggaa ggacauguac acuggggacg accucgauau ugaccgacuc     2520

ucaaacuacg auauugauca cauaauucca caagcauucc uaaaagacaa uucaauagau     2580

aauaaaguac uaguaagcag ugcuaguaau cgaggaaaau cagaugacgu cccuucucuc     2640

gagguaguca agaaaagaaa aacauucugg uaucaacuuc uuaaaagcaa acucauauca     2700

caaagaaagu uugauaaucu cacuaaagca gaacguggag gauuaucacc agaagauaaa     2760

gcagguuuua uacaaaggca acucguagaa acaagacaaa uuacaaagca uguagcacgu     2820

uuguuagacg aaaaauucaa caauaaaaaa gacgaaaaca accgcgcagu acgcacagua     2880

aaaaucauua cuuuaaagag cacacucgua ucccaauuua gaaaagauuu ugaacucuau     2940

aaaguccgag aaauaaacga uuuucaccau gcucacgacg cguauuugaa ugcuguagua     3000

gcaagugcuc uacugaaaaa auauccuaaa cuagaacccg aauucguaua cggagacuau     3060

ccaaaauaca acuccuuccg agaaagaaag agcgccacag aaaaaguaua cuucuauuca     3120

aacauaauga acauauuuaa aaaaaguaua agucuagcag auggucgcgu uauagaaagg     3180

ccauuaauag aagugaacga agaaaccgga gagucaguau ggaacaaaga aagcgacuua     3240

gcaacaguua gacgaguauu aucauaucca caagugaacg uuguaaagaa agucgaagag     3300

caaaaucacg gauuagaucg agguaaaccc aaaggucugu uuaaugcaaa cuuaucauca     3360

aaaccaaaac caaacucaaa cgaaaaccua gucggagcaa aagaauacuu agacccaaaa     3420

aaauacggag gcuacgccgg uauaucaaau aguuuuacgg uucuuguaaa agguacaauc     3480

gaaaaaggag cuaaaaaaaa aauaaccaau guacuugaau uucaagggau aucaauucuc     3540

gaucgcauaa auuaucgcaa agauaaauua aacuucuuac uagaaaaagg auauaaagac     3600

auagaauuaa uaaucgaacu ucccaaauac ucacuguucg aacuaucaga cggaucaaga     3660

agaaugcuag cgaguauacu aucaacuaac aacaaaagag gagaaaucca uaaagguaac     3720

caaaucuuuc uaucccaaaa auuugucaaa cuauuauauc acgcaaaaag aauaucaaac     3780

acuaucaacg aaaaccaucg aaaauauguc gaaaaccaca aaaaggaauu cgaagaauug     3840

uucuacuaua uccuugaauu uaacgaaaac uacguaggcg caaaaaaaaa ugguaaacua     3900

uugaacucug cauuucaaag cuggcaaaac cauaguauag acgaacuuug cucuucauuc     3960

auagguccaa cagguucuga acguaaagga uuauuugaac uuaccucuag agggucagca     4020

gcugauuuug aauuucuagg cguuaaaaua cccagauacc gagauuauac gccgucaucc     4080

uuacugaaag acgcaacacu aauccaucaa aguguaacag gauuguacga aacgcgcaua     4140

gaucuugcaa aauuagggga aggauga                                         4167


<210>  4866
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4866
auggccgcgu uuaaaccaaa cucaauuaau uauauauuag gauuagauau cggaauugcc       60

ucuguagguu gggcgauggu ugaaaucgac gaagaagaaa aucccauaag auuaauagau      120

uuaggaguac guguauucga gcgagcagaa guaccaaaaa ccggcgacuc auuagcaaug      180

gcaagaagau uagcucgauc uguaagaaga cuaacaagaa ggagagccca uagacuguua      240

agaacaaggc gacuuuuaaa acgugaaggu guacuccaag cugccaacuu ugacgaaaac      300

gggcuaauua aaagccuucc aaacacaccc uggcaacuaa gggcagcugc cuuagauaga      360

aaacuaacac ccuuagaaug gucggcaguu cuauuacacc ugauaaaaca uagaggguau      420

uuaucacaaa ggaaaaauga aggugagacc gcagauaaag aacuuggagc auuguuaaaa      480

ggaguagcgg guaaugcaca ugcuuuacaa acaggugacu uuagaacccc cgcagaacua      540

gcacucaaca aauucgaaaa agaaucagga cacauuagaa accaaagauc ugacuacuca      600

cauacauuuu caaggaaaga uuuacaagca gaauuaaucc uauuguucga aaaacaaaaa      660

gaguucggaa acccucacgu aucaggaggu cuaaaagaag gaauagaaac acuccuaaug      720

acgcaaagac cagcgcucuc cggagaugcc guacagaaaa uguuaggaca cugcacuuuc      780

gaaccugcag aaccgaaagc ugcgaaaaac acauauacag cagaacgcuu uauauggcuc      840

acaaaacuua acaauuugag aaucuuagaa caagguucag aaagaccacu gacagacaca      900

gagcgagcua ccuuaaugga cgaaccguau cgaaagagca aauuaaccua cgcacaagca      960

agaaaauugu uaggauuaga agauacagca uucuuuaaag gauuacgaua uggaaaagac     1020

aacgcagaag ccucgaccuu aauggaaaug aaagcguacc acgcaaucuc gagagcuuua     1080

gaaaaagaag gucuaaaaga uaagaaaucc ccacuaaacu uaucaccgga acuacaagau     1140

gaaauaggaa cagcauuuuc acucuuuaaa acagaugaag acauaacagg aagacuaaaa     1200

gauagaauac aaccagaaau auuagaggcu uuacuaaaac auauaucauu ugauaaauuu     1260

guacaaauau cauugaaggc uuugagaaga auuguaccuu uaauggaaca aggaaaaaga     1320

uacgacgaag ccugcgcuga aauauaugga gaccauuaug gaaagaaaaa cacagaagag     1380

aaaauauacu uaccuccaau accagcugau gagauuagaa acccaguagu acuuagagca     1440

cuuucgcaag cucgaaaagu gauuaacggc guaguaagaa gauacggcuc cccggcuaga     1500

auacauauag agacagcaag ggaaguuggc aaaaguuuua aagaccguaa agaaauagag     1560

aaaagacagg aagaaaacag gaaagaccga gaaaaagcag cugcaaaauu cagagaauau     1620

uuuccaaacu uuguaggaga accaaaauca aaagauauau uaaaacugag acuauaugaa     1680

caacaacaug gcaaaugucu auacucagga aaagaaauaa accuuggaag acugaaugaa     1740

aaaggauaug uggagaucga ucaugcgcua ccauucagcc gaacauggga cgauucauuc     1800

aauaauaaag ugcuaguacu aggauccgaa aaucaaaaua aaggcaauca aacgccguau     1860

gaguauuuua acgggaaaga caacucaaga gaauggcaag aauuuaaagc aaggguugaa     1920

accagcaggu ucccucgaag caagaaacaa agaauauuau uacagaaauu cgacgaagac     1980

ggauuuaaag aaagaaaccu uaaugauaca agauauguaa acagguuucu cugucaauuu     2040

guagcagaua ggaugagauu aacugggaaa gguaagaaac gcguauucgc aucaaauggg     2100

caaauaacaa aucuacuaag aggauuuugg ggacuacgaa aaguaagagc cgaaaacgac     2160

agacaucacg cucuagaugc aguagugguu gcauguagua cggucgcaau gcagcagaaa     2220

auaacuagau ucgugagaua uaaagaaaug aaugccuuug auggaaaaac aauagacaaa     2280

gaaaccggag aaguccuaca ucagaaaacg cacuucccac aaccuuggga auuuuucgcu     2340

caagaaguua ugauacgagu auuugguaaa ccagacggca aaccagaguu cgaagaagcu     2400

gauacccuug aaaaauuacg uacccuauua gcagagaaac uaagcagcag acccgaagca     2460

guacacgaau augugacucc gcuauuugua aguagggcac caaauagaaa aaugucuggu     2520

caagggcaua uggaaacugu caaaucagca aaaagacuag augagggagu cucagucuua     2580

agaguacccu uaacacaauu aaaauuaaaa gacuuagaga aaaugguaaa ucgagaaaga     2640

gaaccaaaac uauaugaagc acugaaagcu agacuugaag cacacaaaga ugacccagcg     2700

aaagcauucg cagaaccauu uuauaaauac gauaaagccg gcaauagaac ccaacaaguu     2760

aaagcaguac gaguugaaca agugcagaaa acugggguau ggguccgcaa ucacaaugga     2820

auagcagaua acgcuacaau ggucagagua gauguauucg aaaaagggga caaauauuac     2880

uuaguaccaa uauauuccug gcaagucgcc aaaggaauac uuccugaccg ggcuguaguu     2940

caaggaaaag augaagaaga uuggcaauug auagaugaca gcuuuaacuu caaauucagc     3000

cuacacccaa acgaccucgu ugaagugauc acaaagaaag caagaauguu cgguuauuuc     3060

gcaaguuguc accgugguac agggaacauu aauauaagaa uacacgauuu agaccacaaa     3120

aucgggaaaa acggaauacu cgaaggaauc ggaguaaaaa ccgcuuuguc uuuucaaaaa     3180

uaccaaauug augaauuagg aaaagaaauu agaccaugua gacuaaagaa acguccacca     3240

guuagguga                                                             3249


<210>  4867
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4867
augaaaaagc cuuacaccau uggacuagac auuggaacaa acagcguugg cugggccgua       60

uuaacugauc aauaugaucu agucaaacgu aaaaugaaaa uagccggaga uuccgaaaag      120

aaacaaauca agaaaaauuu uuggggugua cgacuauuug acgaaggaca aacggcagcc      180

gaccgcagaa uggccaggac ggccaggaga agaauagaaa gacgacguaa ccgcauauca      240

uaccuccaag gaauauucgc agaagaaaug ucuaaaaccg augccaacuu cuuuugccgc      300

uuaagcgauu cauuuuacgu agacaaugag aaaagaaacu cccgucaucc uuucuuugcc      360

acaauagaag aagaaguaga auaucauaaa aauuacccua caauuuauca ucuaagagaa      420

gaacugguua acuccagcga aaaagcagac cuaagauuag ucuaccucgc gcucgcgcau      480

auaauuaaau auagaggaaa cuuuuuaaua gaaggagcuu uagauacaca aaauacgagu      540

guggauggua uauacaaaca auuuauccag acguauaauc aaguauuugc uaguggaauc      600

gaagacggcu cacuuaagaa acucgaagac aauaaagaug uagccaaaau ucuagucgag      660

aaagucacga ggaaagaaaa acuagaaaga auacucaaau uauacccagg ggagaaaagc      720

gcuggaaugu uugcacaauu uauaucucuc auaguaggaa gcaaagguaa uuuucaaaaa      780

ccauuugauu uaauagaaaa aucggauauc gaaugugcaa aagauagcua ugaagaagau      840

cucgaaucac ucuuggcguu gauuggcgac gaauacgcag aacuguuugu cgcagccaaa      900

aaugcauaca gcgcagucgu ccucucaagu auaauaaccg uagcagaaac cgaaacuaau      960

gcaaaacugu ccgcaucaau gauagaaaga uucgauacgc augaggaaga ucuaggugaa     1020

uuaaaagccu uuauaaaauu gcacuuacca aaacacuaug aagaaauauu uagcaauacc     1080

gaaaaacacg guuacgcagg auacaucgau ggcaaaacaa aacaagcaga uuucuacaaa     1140

uacaugaaga ugacacuaga aaacauagaa ggugcagacu acuucauagc aaagauagag     1200

aaggaaaacu uccucagaaa gcaacguacu uuugauaaug gugcuauccc acaccaauua     1260

cacuuagaag aacuagaggc aauauuacac caacaagcua aauacuaucc guuucucaaa     1320

gaaaacuaug acaaaauaaa aucauuagua acauuuagga uuccauauuu cguuggaccc     1380

uuggcaaacg gacaauccga guucgcaugg cuaacuagga aagcggacgg ggaaauccga     1440

cccuggaaua uugaagaaaa aguagacuuc gguaaaagug caguagauuu cauagagaaa     1500

augacaaaca aagauacaua ucuaccaaaa gaaaacguuu uaccaaaaca uaguuuaugu     1560

uaccaaaaau accuaguaua uaaugaacuc acaaaaguua gguauauuaa cgaccaaggu     1620

aaaacaucgu auuuuagcgg acaagaaaaa gaacaaauau ucaaugaccu auucaaacaa     1680

aaacgaaaag uaaagaagaa agaucucgaa cuauucuuaa gaaauaugag ucacguagaa     1740

ucaccaacua uagaaggacu agaagacagu uuuaauuccu ccuauaguac auaccaugau     1800

cuacucaaag uagggauaaa acaagaaauu uuagacaacc caguaaauac agaaauguua     1860

gaaaauaucg ucaaaaucuu aacaguauuu gaagauaaac gaaugauaaa agaacaacuc     1920

cagcaauuuu ccgauguacu cgacgguguc gucuuaaaga aacuugaaag aagacauuau     1980

accggauggg gacgauuauc agcaaaacua uuaaugggca uucgagacaa acagagccac     2040

cuaacaaucu uggauuaccu aaugaaugac gauggacuca accgaaauuu gaugcaacuc     2100

auaaacgaua guaacuuauc uuucaaauca aucauagaaa aagaacaagu cacaacugca     2160

gacaaagaua uccagagcau agucgcugau cuagccgguu cacccgcuau uaagaaaggc     2220

auauugcaau cauuaaaaau aguggacgaa uuaguaucag ucaugggcua cccaccgcaa     2280

acaauugucg uagaaauggc uagagaaaau caaacaacag gaaaaggcaa aaauaauagc     2340

agaccuagau acaaaucacu ugaaaaagcc auaaaagagu ucggcucuca gauauuaaaa     2400

gaacauccca cagauaauca ggaacugagg aacaauagau uauaucuaua uuacuugcag     2460

aaugguaaag auauguacac uggacaagac cuugacauac acaauuuauc aaauuacgau     2520

aucgaccaca uagugcccca aaguuuuauu acugacaauu cgauugacaa ucuaguacuc     2580

acaucaucag cuggaaauag ggaaaaaggc gaugauguac caccacuaga aaucguacga     2640

aaacgaaaag uauuuuggga aaaauuauac caaggcaauu uaaugaguaa aagaaaauuc     2700

gacuaucuua caaaagcuga acgaggagga cucacugaag ccgacaaagc aagauuuaua     2760

cacagacaau uaguagaaac gcggcagaua acuaaaaacg uagcuaacau acuacaccaa     2820

agauucaacu acgaaaagga cgaccacgga aacacaauga aacaaguaag gauagugaca     2880

uuaaaauccg cgcuaguaag ccaauucaga aaacaguuuc aacuauacaa aguuagagac     2940

guaaaugauu aucaucaugc ucaugaugcg uaccucaacg gcguuguggc aaauacauua     3000

uuaaaagugu auccacaauu agaaccagaa uuuguuuacg gagauuacca ucaauucgac     3060

ugguuuaaag caaauaaggc uacagcgaag aaacaauucu acacuaauau uaugcuauuc     3120

uucgcacaaa aagauagaau cauagaugaa aauggagaaa uucuuuggga caagaaauac     3180

cucgauacag uuaagaaagu aaugucauac agacaaauga acauagucaa gaaaacggaa     3240

auucaaaaag gagaguucuc gaaagcaacc auaaaaccga aaggaaauuc cagcaaacuc     3300

auacccagaa aaacaaauug ggauccaaug aaauacggag gcuuagacuc gccaaauaug     3360

gcauaugccg uagucauaga auacgcuaaa gguaaaaaca aauuaguauu cgaaaagaaa     3420

auaauuagag ucacaauaau ggaaagaaaa gcauucgaaa aagacgaaaa agcguuucua     3480

gaagaacagg gauauagaca gcccaaagua uuagcaaaac uaccuaaaua uacguuauac     3540

gaaugugaag aaggaagaag gagaaugcug gcaucugcca acgaagcuca aaaaggaaac     3600

caacaaguau uaccuaauca uuuaguaaca cuucuacauc augcggccaa cugcgaaguu     3660

ucggacggaa aaagucuaga cuacauagaa ucaaacagag aaauguuugc ggaauuacua     3720

gcucacguua gugaguucgc aaaacguuac acuuuagcug aagcaaacuu aaacaaaaua     3780

aaccaauuau ucgaacagaa uaaagaaggc gauauuaaag ccauagcuca aaguuuugua     3840

gaucuuaugg cuuuuaaugc aaugggggca ccagccucau ucaaauuuuu cgaaacuacc     3900

auugaaagga aaagauauaa caaucuuaaa gaacuacuca acucaacaau aauauaccaa     3960

ucaaucacag gauuauauga aucacgaaaa agauuagaug acuga                     4005


<210>  4868
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4868
augaaaaaac cauacucgau ugggcuugac auagguacaa auucagucgg cugggcaguc       60

guaacggaug acuacaaggu uccagccaaa aaaaugaaag ugcuaggcaa uacagacaaa      120

ucacauauag aaaaaaaccu uuugggcgca cuauuauucg acucaggaaa uacagccgaa      180

gaccgucgac uaaaaaggac agcccguaga agauauacaa gacgaagaaa cagaaucuua      240

uacuuacaag aaauauuuuc ugaagaaaug ggaaaaguug augauucauu cuuccaccga      300

cuagaagaca gcuucuuggu aacugaagac aaacgaggag agagacaccc aauauucggc      360

aaccucgaag aagaagucaa auaucacgaa aauuuuccca caauauauca uuuacgucag      420

uauuuagcgg auaacccuga aaaaguagau uuacgcuuag uauaccucgc acuagcccac      480

aucauaaagu ucagaggaca cuuucuuauu gagggcaaau ucgacacaag gaacaaugac      540

guucaacgac uauuccaaga auuuuuagca guguacgaua acaccuucga gaauagcucc      600

cuccaagaac aaaacguuca agucgaagaa auacuaaccg auaaaaucuc aaaauccgca      660

aaaaaagaua gaguacugaa acuauucccg aaugaaaaaa gcaauggacg auuugcagaa      720

uuuuugaaac uaaucguagg caaccaagcc gacuuuaaaa aacacuuuga auuagaagaa      780

aaagcuccau uacaauuuag uaaagacaca uacgaagaag aacucgaagu auuauuggcu      840

caaauuggug auaacuacgc agaacuauuu cuaucugcaa aaaaauuaua ugacucuauu      900

cuacuaucag gcauacuuac uguuacagac guaggaacga aagcgccauu auccgcgagc      960

augauacaac guuauaacga acaccagaug gacuuggcac agcuaaaaca auuuauucga     1020

caaaaauuga gugacaaaua caacgaagug uuuagcgacg uaucaaaaga cgguuaugca     1080

gguuacauag augguaaaac aaaucaggaa gcauucuaca aauacuuaaa aggccuacua     1140

aacaaaauag agggaucagg auauuuccua gacaaaauag aaagagagga uuuccuucgu     1200

aaacaaagaa cauucgacaa uggaucuaua ccacaccaaa uacaccugca agagaugaga     1260

gcaauuauac ggcgccaagc agaauuuuac cccuucuuag ccgauaauca agauagaauc     1320

gaaaaacuau uaacauucag aaucccauac uacguaggac cguuagcacg aggaaaaagc     1380

gauuuugcau gguuaucuag aaaguccgcc gauaaaauca cacccuggaa cuuugaugaa     1440

aucguagaca aagaaucauc ggcagaagcc uucauuaacc ggaugacaaa uuaugacuua     1500

uauuuaccaa accaaaaagu ccuacccaaa cacuccuuau uauaugagaa auuuaccgua     1560

uauaacgaac ucacaaaagu caaauauaaa acagaacaag gaaagacagc uuuuuuugau     1620

gcgaauauga agcaagaaau auuugauggc gucuucaaag uauaccgaaa aguaacuaaa     1680

gauaaauuaa uggacuuccu agagaaggaa uucgaugaau uuagaauugu agaccuaacg     1740

gggcuagaca aagaaaauaa ggucuucaac gcaagcuaug gcaccuauca ugaucuuugu     1800

aaaauauugg acaaagauuu uuuagauaau ucaaagaaug aaaaaauacu ggaagacaua     1860

guauuaacac uuacccuuuu ugaagaucgc gagaugauaa gaaaaagauu agaaaacuac     1920

agugaucucc uaacaaaaga acaaguaaaa aaauuagaaa gaaggcacua caccggaugg     1980

ggaagacuau cugcggaauu aauacaugga auuagaaaca aagaaagcag aaaaacuaua     2040

cuagauuacc uaaucgacga cgguaauagc aauagaaauu ucaugcaacu aauaaacgac     2100

gacgcacuau ccuuuaaaga agaaauagcu aaagcucagg uaauaggcga aacugauaac     2160

uuaaaucaag uaguaucuga cauagccggc agcccagcaa uaaaaaaggg uauccuucaa     2220

ucuuuaaaaa ucgucgauga acuaguaaag auuaugggcc accaaccaga aaacauaguc     2280

guagagaugg cacgcgaaaa ccaauuuacg aaccaaggaa gaagaaauuc acaacaacga     2340

cuuaagggau uaaccgauuc uauaaaagaa uuugggucac aaauauugaa agaacaucca     2400

guagaaaauu cacaauuaca aaacgaccga cuauuucuuu auuaucuuca aaauggacgu     2460

gauauguaca ccggugaaga acuugauaua gacuaucuaa gccaauauga cauugaccac     2520

auaauuccac aagcuuuuau aaaagauaau uccauagaua auaggguacu cacgucauca     2580

aaagaaaaca gaggaaaauc agaugauguu ccuagcaaag auguuguaag gaaaaugaaa     2640

agcuacuggu caaagcuccu aucugcaaaa uuaauaacac aaagaaaauu cgacaaccuu     2700

acaaaagcgg aacgaggagg ccucacagac gacgacaaag caggauucau caaaaggcaa     2760

cuaguagaaa cuagacaaau cacaaaacac guagcuagaa uauuagacga acgauuuaac     2820

accgaaacug acgaaaauaa caagaaaauc aggcaaguca aaauagucac auugaaauca     2880

aaccucgucu ccaacuuuag aaaagaauuc gaacuuuaua aaguacgaga aauaaacgau     2940

uaccaucacg cacacgacgc auaccuuaau gcaguaauag gaaaagccuu acuaggugua     3000

uacccccaac uagagccaga guuuguauac ggcgacuacc cacacuuuca uggccacaaa     3060

gaaaacaaag caacugcaaa aaaauucuuc uauaguaaca uaaugaauuu uuuuaaaaaa     3120

gacgacgucc ggaccgauaa gaauggcgaa auaauaugga agaaagauga acacauaagu     3180

aacauaaaaa aaguacuuuc cuacccucaa guaaacaucg uuaaaaaagu agaagagcaa     3240

acgggaggcu ucaguaaaga aagcauauua ccaaaaggaa auucugacaa acuaauccca     3300

agaaaaacua aaaaauucua uugggacaca aaaaaguacg ggggauuuga cuccccuauc     3360

guagcauauu caauccuagu aauugcagau aucgaaaaag gaaagucgaa aaaacuaaaa     3420

acaguaaaag cuuugguagg gguaacaauu auggaaaaaa ugaccuuuga aagagaucca     3480

guggcuuucc uagaaagaaa agguuacaga aacguacaag aagagaacau uauaaaacuc     3540

ccaaaauaca gccuauuuaa acuugaaaac ggaagaaaaa ggcuacuggc aucggcucga     3600

gaacugcaga agggaaacga aauagugcua ccaaaucauu uaggaaccuu gcuauaucac     3660

gcaaaaaaua uccauaaggu cgaugaaccg aaacacuuag auuacguaga uaaacauaaa     3720

gaugaauuca aagaacuccu agauguggua aguaauuuua gcaaaaaaua uacccuugca     3780

gagggaaacc uagaaaaaau aaaagaacuc uaugcucaaa acaacggcga agauuuaaaa     3840

gaacuagcuu caucauucau uaaucuacua acauucacag caaucggugc cccagcaaca     3900

uuuaaauuuu uugacaaaaa caucgacaga aaacgauaua caucuacaac ggaaauacuc     3960

aaugcaacac uaauccauca aucaauuacu ggccucuacg aaacucgaau ugaccuuaau     4020

aaacucggag gcgauuga                                                   4038


<210>  4869
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4869
augcaaacua caaaccuguc guacauauua gggcuagacc uaggaaucgc uagcguaggc       60

ugggcugucg uagaaauaaa ugaaaaugaa gacccaauag gacuaaucga cguaggugug      120

cgaaucuucg aaagagcaga aguuccuaaa acaggagaau cgcuagcacu cucacgucga      180

cuugccagau ccacccguag guugauacgu agacgugcac acagacuacu auuagcgaaa      240

agauuucuaa aaagagaagg cauacuuucc acaauagauc ucgaaaaggg auuaccuaau      300

caggcuuggg aacuuagagu agcaggacuu gaacgucgau uaagugcaau ugaaugggga      360

gcaguacuau ugcaccuaau aaaacauaga ggcuaucucu caaaaagaaa gaaugaaagc      420

caaacgaaca auaaagaacu aggagcauug cuaagcggug uagcucaaaa ccaucaacua      480

cugcaaagcg augauuacag aacaccagcc gaauuagcac uaaagaaauu cgcaaaagaa      540

gaaggccaua ucagaaacca acgaggggca uauacgcaca cuuucaacag acuagauuua      600

cucgcugaau ugaaccuauu auucgcacaa caacaucaau uuggcaaucc acauuguaaa      660

gaacacauac aacaauacau gacagaacua cuuauguggc aaaagccagc ccucucuggc      720

gaggcaauuc uaaagaugcu aggcaaaugc acacacgaga aaaaugaauu uaaagcagca      780

aaacauacuu acuccgccga aagauuugua uggcuuacua aacuuaacaa cuuaagaauc      840

cuagaagacg gcgcagaaag agcacuaaau gaagaagaac gacaacuauu aauaaaucac      900

ccuuacgaaa aaucgaaacu aacauacgcg caaguaagaa aacuucucgg uuuaucagaa      960

caagcaauau ucaaacauuu aagauauucu aaagaaaaug cagaaagcgc aaccuucaug     1020

gaauuaaaag cauggcacgc uauaagaaaa gcucuugaaa accaaggguu aaaagauaca     1080

uggcaagauc uagcaaagaa acccgacuua cuagacgaaa ucggaacggc uuuuagccua     1140

uauaaaacag augaagauau acaacaauac cucacgaaua aaguaccaaa caguguuaua     1200

aacgcacuau uagucucauu aaacuuugau aaguuuauag aguuaucacu aaaaucgcuc     1260

cgaaaaauau uaccucuaau ggaacaaggu aaacguuacg accaggcuug ccgugaaaua     1320

uauggacauc acuacgggga agcaaaucag aaaacuucac aacuauuacc cgcaauaccu     1380

gcccaagaaa uaagaaaucc gguaguacua agaacacuau cacaagcacg aaaaguaaua     1440

aaugccauaa uaagacaaua cggaucucca gcaagaguac auauagaaac agggagagaa     1500

uuagguaaau cuuucaaaga acgaagggaa auccaaaaac aacaagaaga uaauagaaca     1560

aaaagagaau cagcuguaca aaaguuuaaa gaacuauucu ccgauuucuc uucggaacca     1620

aaaaguaaag auauauuaaa auuuagacuc uaugaacaac aacacggcaa augcuuauau     1680

ucaggcaaag aaauaaauau acaucgacuc aaugaaaaag gauacguuga gaucgaccac     1740

gcacuuccgu uuaguaggac cugggaugac ucauuuaaca auaaaguccu aguauuagca     1800

ucagagaacc aaaauaaagg aaaucaaacu ccuuacgaau gguuacaagg caaaaucaac     1860

ucagaacgau ggaaaaacuu cguagcauua guauuaggaa gucagugcuc ggcggccaag     1920

aaacaaagac uacuuacgca aguaauugau gacaacaaau ucaucgauag aaaccuaaac     1980

gacaccagau acauagcgag auuuuuaucc aauuacauac aagaaaauuu auuauuagua     2040

gguaaaaaca agaaaaaugu auucacaccc aacggacaga uaacagcgcu auuacgaucu     2100

cgauggggau uaauaaaagc ccgagaaaau aacaacagac accacgcauu agaugcaaua     2160

guaguagcuu gcgcaacacc uagcaugcaa cagaaaauua cccgauuuau cagauucaaa     2220

gaagugcacc cuuauaaaau ugaaaaccga uacgagaugg uagaccaaga gagcggagag     2280

aucauauccc cacacuuucc agaaccaugg gcauauuuca gacaagaagu caacauacga     2340

gucuuugaua accauccaga cacugugcug aaagaaaugu uacccgaucg cccacaagca     2400

aaucaucaau uuguacaacc ccuuuucguu ucaagagccc caacuagaaa gaugagugga     2460

caaggacaca uggagacuau aaaaucggcc aaacgacuag ccgaaggaau auccguacua     2520

agaauacccu uaacacaacu aaaaccaaau cuacuggaaa auaugguaaa caaagaaaga     2580

gaaccagcuc uauacgcagg ccuaaaagca agacuagcag aguucaacca agauccagca     2640

aaagcauucg caaccccauu cuacaaacaa ggaggacaac aaguuaaagc aauccgaguc     2700

gaacaagucc aaaaaucugg aguacugguu cgcgaaaaca acggaguagc agacaaugcg     2760

ucaauuguaa gaaccgacgu auucauuaaa aacaauaaau ucuuucucgu acccauauac     2820

acauggcaag uggcuaaggg gauucuaccc aacaaagcua uaguagccca uaaaaaugaa     2880

gacgaauggg aggaaaugga cgaaggagca aaauuuaaau ucucucuuuu uccuaacgac     2940

cuugucgaau uaaaaacaaa gaaagaauac uuuuucggau auuacaucgg auuagacaga     3000

gcaaccggaa acauaagucu aaaagaacac gacggagaaa uaagcaaagg aaaagacgga     3060

guauauagag uaggaguuaa gcucgcauua ucauuugaaa aauaucaagu cgacgaacug     3120

ggcaaaaauc gacaaauaug ccguccccaa caaagacaac cagucagaug a              3171


<210>  4870
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4870
auggccgccu ucaaaccaaa uccuauaaac uauaucuuag gcuuagacau aggaauagca       60

uccguuggau gggcaauggu ggaaauagau gaagaugaaa auccuauaug ccuaauagau      120

uuaggaguua ggguauuuga aagagccgaa guaccaaaaa caggagauag ccuugccaug      180

gcgagaagau uagcaagauc uguaagaagg cuaacacgac ggagagcaca uagacuacua      240

cgagcaagaa gacuauuaaa gagagaggga guuuuacaag cagcagacuu ugaugagaau      300

ggauuaauaa agucccuucc uaauacacca uggcaacuuc gagcagcugc cuuagacagg      360

aagcuaacac cauuggagug gucagcugua uuauuacauc uaauuaaaca uagggguuau      420

uuaucucaaa ggaaaaacga aggagaaaca gcugacaaag aacuaggggc acuacuaaaa      480

ggcguggcag acaaugccca cgcauuacaa acaggagauu uuagaacccc ugcagaauua      540

gcacuaaaua aguuugaaaa ggaaucagga cacaucagaa aucaaagagg ggauuauuca      600

cauacauuuu ccagaaaaga ucuucaagca gaacucauuu uauuauuuga aaaacaaaaa      660

gaauuuggga auccacaugu aagcggagga cuaaaagaag gaauagaaac auuacuuaug      720

acacaaagac cagcacuaag uggggaugca guccagaaaa uguuaggaca uugcacauuu      780

gaaccugccg aaccaaaagc ugcgaaaaac acauacacag cagaaagauu uauuugguua      840

acaaaacuca acaaucuaag aauacucgaa caagguucug aaaggccauu aacugacaca      900

gaaagagcga cacuaaugga cgaaccauac aggaaaucaa aauugacuua ugcacaagcc      960

agaaaauugu uaggacuaga agauaccgcu uucuuuaaag gacugagaua ugguaaagau     1020

aaugccgaag cgucaacccu gauggaaaug aaagcauauc acgcuauaag uagagcucug     1080

gaaaaagaag gauuaaaaga caagaaaucu ccauuaaacc uaucgccaga auuacaagau     1140

gaaauaggua cugcauuuuc ccuuuuuaaa acagacgaag auauuacggg aagauuaaag     1200

gaccgcaucc aaccagaaau uuuagaggcu uuacuaaaac acauaucauu ugauaaguuc     1260

guucaaauaa gucugaaagc auuaagaaga auaguuccuu uaauggaaca agggaaaaga     1320

uaugaugaag caugugcaga aauauauggu gaccauuacg ggaagaaaaa cacggaagag     1380

aaaauauauu ugccaccaau accagcugau gaaauuagga acccagucgu auuacgagca     1440

uuaucacaag caagaaaagu uaucaacggu guagugcgac gauaugguag cccggcucga     1500

auacacauag aaacagcaag agaaguagga aaaaguuuua aagacagaaa agaaauagag     1560

aaaagacaag aagaaaaucg caaagauaga gaaaaagccg ccgcaaaauu uagagaauac     1620

uuccccaauu uuguagguga accaaaauca aaagacauac uuaaacuaag acuauacgaa     1680

caacagcacg gaaaauguuu auauuccggc aaagaaauca aucuugguag acucaaugaa     1740

aaagguuaug uagaaauaga ucaugcacua ccguuuagca gaacauggga ugauagcuuc     1800

aauaauaaag uacuuguauu aggaagugaa aaucaaaaua aagggaauca aacaccauau     1860

gaauacuuca acggaaaaga uaacagcaga gaauggcaag aguucaaagc ucgaguagaa     1920

acaagcagau ucccucguag uaagaaacaa cgcauauugc uccaaaaauu cgacgaagau     1980

ggcuucaaag aaagaaaucu aaaugauacg agauacguca auagauuuuu gugucaauuu     2040

gucgcugaca ggaugagacu aacagguaaa ggcaaaaaga gaguauucgc uagcaaugga     2100

caaauaacua acuuauugcg cggauuuugg ggauuaagaa aaguaagagc agaaaacgac     2160

agacaccaug cauuggacgc agucguagua gcaugcagca caguagcaau gcaacaaaag     2220

auaacuagau ucguacgaua uaaggaaaug aacgcauucg augggaaaac aauugauaag     2280

gaaacaggug aaguacuaca ucagaagacu cauuuuccac aaccuuggga auuuuucgcc     2340

caagaaguua ugauucgagu auuuggaaaa ccagauggaa aaccagaauu ugaagaagcc     2400

gauacuccag aaaaacuacg cacauuacuc gcggaaaaac uaucaagcag accagaagca     2460

guucaugaau auguuacucc acuuuucgua ucaagagcac cuaauagaaa gaugucgggu     2520

caaggacaca uggaaacagu aaaaagugcc aaaagacuag augaaggcgu aaguguguua     2580

agaguucccu ugacgcaacu aaaacuaaaa gauuuagaga aaauggucaa uagagaaaga     2640

gaacccaaau uauacgaagc acucaaagca agauuagaag cacauaagga ugauccagca     2700

aaagcauuug cugaaccuuu cuauaaauau gauaaagcgg guaacagaac ccaacaaguu     2760

aaagcaguaa gaguugaaca aguacagaaa acaggcguuu ggguaagaaa ccacaacgga     2820

auagcagaua acgcuacaau gguaagagua gauguguuug aaaagggaga uaaguacuau     2880

uugguaccaa ucuauagcug gcaaguugcu aaagggauac uaccugaccg ugcaguagua     2940

caaggaaaag augaagaaga cuggcaacua aucgacgacu cauuuaauuu caaauuuagu     3000

uuacacccua acgacuuagu cgaaguuaua acuaagaaag cuaggauguu uggauauuuu     3060

gcaagcugcc auaggggcac uggaaauaua aauauaagaa uacacgauuu agaucauaaa     3120

auugguaaaa acggaauauu agaaggaaua ggcgucaaaa cugcauuauc uuuucaaaaa     3180

uaucaaauag augaauuagg aaaagaaauu agaccaugua gacucaagaa acgaccucca     3240

guccgauga                                                             3249


<210>  4871
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4871
augaaauauc auguagguau agacgucgga acauuuucag uuggacuggc ugcaaucgaa       60

guagaugacg caggcaugcc cauaaagacu cuaaguuuag uaucucacau acaugauagc      120

ggauuagacc cagaugaaau aaaaagugca gugacaagac uugccaguag ugguauagca      180

agaagaacaa gaagauuaua cagaagaaag agaagaagau uacaacaguu agauaaguuu      240

auacaaaggc aaggguggcc aguuaucgaa cuagaagauu acagugaucc acuauaucca      300

uggaaagugc gagcugaauu agccgcuagc uauaucgcug acgaaaaaga aagaggagaa      360

aaacuaucag ucgcccuucg ccauauugca cgacacagag gcuggcgaaa ccccuaugcc      420

aaaguaaguu cacuauauuu accugauggu ccaucugaug cuuuuaaagc aauaagagaa      480

gaaauuaaaa gggcaagcgg ucaaccugua ccggaaacag cuacaguagg acaaaugguu      540

acgcuaugug aguuaggaac uuugaaacua aggggagaag gagggguacu aagugcaaga      600

uuacaacaaa gugauuaugc aagggaaauu caagaaauuu guagaaugca agagauaggu      660

caagaauuau acagaaaaau uaucgauguu guuuuugcag cggaaagucc gaaaggaagu      720

gcuagcagcc guguagggaa ggauccauua caaccaggua aaaaucgcgc auuaaaagca      780

agcgaugcgu uucaacggua cagaauagca gcauugaucg gaaacuuaag aguaagagug      840

gauggggaaa agagaauccu uucgguagag gagaaaaauc uaguauuuga ucaucuaguu      900

aacuuaacac cuaagaaaga accagaaugg guaaccauag cggaaauacu agguauagau      960

cggggacaau uaauaggaac cgccacuaug acugaugaug gugaaagagc cggagccaga     1020

ccuccaacuc acgacacuaa uagaucaauc guaaauagua gaauagcacc uuuaguagau     1080

ugguggaaaa cggcgucugc acuagaacaa caugcuaugg uaaaagccuu aaguaaugcc     1140

gaaguugaug acuuugauuc uccggaagga gccaaaguac aagcauucuu ugcugaucuu     1200

gaugacgacg uacaugcuaa acuagauuca cugcauuugc ccguagguag ggcggcguac     1260

agugaagaua cucuaguacg auuaacaaga cguauguuaa gugauggggu agaucuauac     1320

acugcaaggc uacaggaguu cgggauagaa ccgaguugga cgccacccac accaagaaua     1380

ggugaacccg uaggcaaucc agcuguagau aggguucuaa aaacaguaag uagguggcuu     1440

gaaucagcaa cuaaaacaug gggugcucca gaaagaguga uuauagaaca ugugagagaa     1500

ggguucguca cagagaaaag agcuagggaa auggacggag auaugaggcg uagagcggcc     1560

agaaacgcaa aauuauucca agaaaugcaa gaaaaauuga acguacaagg aaaaccuuca     1620

agagcugauc uauggagaua ucaaagugua cagagacaaa auugccaaug cgcauacugu     1680

ggaagcccaa ucacauuuag caauucggaa auggaccaua uaguaccucg agcgggucaa     1740

ggaucaacaa auacucgaga aaaucuagua gcaguauguc auagaugcaa ucagucaaaa     1800

gggaauacac cauuugcaau cugggcgaaa aacaccagua uagaaggugu aagcguaaaa     1860

gaagcaguag aacgaaccag gcauugggua acagauacag gaaugcgaag cacggacuuu     1920

aaaaaguuua caaaagcagu uguagaaaga uuccaaagag caacaaugga cgaagaaaua     1980

gaugcucgau caauggaauc aguagcuugg auggcaaacg aacuaagauc uagaguagca     2040

caacauuuug caucacaugg aaccacggua agaguauaua gagguaguuu aaccgcagaa     2100

gcuagacgag caucaggaau uaguggaaaa cuaaaauucu uugauggagu ugguaagucg     2160

agauuagaua gaagacauca ugcgauagau gcggcuguaa uagcauuuac aagugacuau     2220

guggcagaaa cuuuagcugu aagaaguaac uuaaaacaau cgcaggcaca uagacaggaa     2280

gcuccacaau ggagagaguu uacaggaaaa gaugccgaac aucgggcggc auggagaguu     2340

uggugucaga aaauggaaaa acuuagcgca cuacuaacag aagaccuuag agacgaucgg     2400

gugguuguua ugaguaaugu uagacuacga uuaggaaaug gaagcgccca caaggaaacg     2460

auagggaaac uaucaaaagu uaaauuauca ucacaacuga gugugucaga uauagacaaa     2520

gcaucauccg aagcauugug gugugcauua acucgagaac caggauuuga uccaaaagag     2580

ggucuaccgg caaaucccga acggcauaua aggguaaacg gcacacacgu cuaugccggu     2640

gauaauaucg gguuauuccc gguaagcgca ggaaguaucg cacuaagagg gggauaugca     2700

gagcuaggcu cauccuucca ucaugcacga guguacaaaa uuacgagcgg caagaaacca     2760

gcauuugcua ugcuuagggu auauacaauu gaucuauuac cauauagaaa ccaagauuua     2820

uuuaguguug aacuaaaacc acaaacaaug ucaaugagac aagcagaaaa gaaauuaaga     2880

gaugcuuuag ccacuggaaa ugccgaauau cucggauggc ugguuguuga ugaugaacuu     2940

gucguagaua cuucaaaaau agccaccgau caagugaaag caguagaagc ugaacuaggg     3000

acuauuagaa gauggagagu agacggcuuc uuuagcccau caaaauuaag gcuaagacca     3060

cuacaaaugu cuaaagaagg cauuaagaaa gaaagcgccc cagaauuauc aaaaauuaua     3120

gauagaccgg guugguuacc cgcugugaau aaacuauuuu ccgaugguaa uguaacaguc     3180

gugagacgug acucucuugg ucgaguaaga cuagaaagua cugcgcaccu accaguuaca     3240

uggaaagugc aauga                                                      3255


<210>  4872
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4872
auggcaagaa uucucgcauu ugauaucgga aucucaucaa uaggaugggc auucagcgaa       60

aacgacgaac uaaaagauug uggggugcgc auauuuacaa aagucgaaaa uccaaaaacu      120

ggagaaucgu uagcacuacc acgaagauua gcucgaucug cccgaaaaag acuugcccga      180

aggaaagcaa gacuuaauca ccuaaaacau cuaauagcaa acgaauucaa acuaaauuac      240

gaagacuauc aaagcuuuga cgaaucauua gccaaagcau auaaaggaag ccucauaucu      300

ccauaugaau uaagauuuag ggcucugaac gaacuauuau caaaacaaga cuuugcaaga      360

guaauauuac acauagcaaa acgaagagga uacgaugaca ucaaaaauuc cgacgauaaa      420

gaaaaaggag cuauacuaaa agcaauuaaa caaaacgaag aaaaacuugc uaacuaccaa      480

ucagugggag aauaccuaua uaaagaauac uuccaaaaau ucaaagaaaa uagcaaagaa      540

uucacaaaug ucaggaacaa aaaagaaagu uaugaacgau gcauagcuca aucauucuua      600

aaagaugaau uaaaacuaau auuuaaaaaa caaagagaau ucggauuuuc cuucagcaaa      660

aaauuugaag aagaaguacu uagcgucgca uuuuauaaaa gggcacucaa agacuucagc      720

caccuaguag ggaacugcuc auucuuuacc gaugaaaaaa gagcaccaaa aaauucgccc      780

uuagccuuca uguucguagc acucacaagg auaaucaauc uacuaaauaa uuuaaaaaac      840

acagaaggaa uacuuuauac aaaagacgau cuaaacgcau uacuuaacga aguacucaaa      900

aauggaacuc uuacauacaa gcaaacaaaa aaacuacuag gacuauccga cgauuacgaa      960

uuuaaaggag aaaaaggcac auauuucauu gaauucaaaa aauacaaaga guuuauaaaa     1020

gcucucggag agcauaaucu aucccaagac gacuuaaacg aaaucgccaa agacauaacc     1080

uuaauaaaag acgagauaaa guuaaaaaaa gcacucgcaa aauacgaucu gaaccagaac     1140

cagauagaua gccucucaaa acuggaguuc aaggaccacc ucaauauaag cuucaaagca     1200

uuaaaacuag uaacuccccu gaugcuggaa ggaaaaaaau augaugaagc cugcaacgaa     1260

uugaaccuaa aaguagccau caaugaagac aaaaaagauu ucuuaccagc auuuaaugaa     1320

acauacuaua aagaugaggu uacaaauccc guaguacuua gagcaauuaa ggaguaccga     1380

aagguuuuaa augcucuacu uaaaaaauac gggaaaguac acaaaaucaa cauagaguua     1440

gcacgagaag uagggaaaaa ucacucucaa agggccaaaa uagaaaaaga acaaaacgaa     1500

aauuauaaag caaaaaaaga ugcagaacua gaaugcgaaa aacuaggauu aaaaauaaac     1560

uccaaaaaua uauugaaauu acgucuauuc aaagaacaaa aagaguuuug cgcauauuca     1620

ggagaaaaaa ucaaaaucuc agauuuacag gaugaaaaga ugcuagaaau ugaccacaua     1680

uacccauacu ccagaucauu cgaugauagc uacaugaaca aaguacuagu uuucaccaag     1740

caaaaccaag aaaagcuaaa ucaaacacca uucgaagccu uuggaaauga cagcgcaaaa     1800

uggcaaaaga uagaaguauu agccaaaaac cucccaacaa aaaagcaaaa aaggauacuc     1860

gacaaaaacu auaaagacaa agaacaaaaa aacuuuaaag auagaaacuu aaaugacacc     1920

agguacauug cucgacucgu auuaaacuac accaaagacu accuagacuu ccuuccccuc     1980

ucagacgacg aaaacacuaa gcuuaacgac acccaaaaag gaagcaaggu acaugucgaa     2040

gcuaaaucag gaaugcuuac cuccgcacuu cgacauacau ggggcuuuuc agcaaaagac     2100

aggaacaauc accuacacca cgccaucgau gcagucauca uagcauacgc aaacaauagc     2160

auuguaaaag cauuuagcga uuucaaaaaa gaacaggaaa gcaauucagc cgaacuguau     2220

gcaaaaaaaa uuagcgaacu agauuacaaa aauaaaagaa aguucuuuga accuuucuca     2280

ggauucagac aaaaaguacu cgacaaaaua gacgaaauau ucguaaguaa accagagaga     2340

aagaaaccua guggagcauu acacgaagaa acauucagaa aagaagaaga auucuaccaa     2400

ucauauggug gcaaagaggg aguacuaaaa gcacuagaac uagguaaaau acgcaaagua     2460

aacggaaaaa uaguaaaaaa uggcgacaug uuuagagucg auauauucaa gcacaaaaaa     2520

acaaacaaau uuuaugcagu ucccauauau accauggauu uugccuuaaa aguccuacca     2580

aauaaagccg uugcaagauc aaaaaaagga gaaauuaaag acuggaucuu aauggaugaa     2640

aacuacgaau uuuguuuuuc cuuauacaaa gacagccuua uccuuauaca aaccaaagau     2700

augcaggaac cugaauuugu cuauuauaau gcuuucacca gcucaaccgu uucacuaaua     2760

gugucaaaac augauaacaa guucgaaaca cugaguaaga accaaaaaau auuguuuaaa     2820

aacgcaaaug aaaaagaagu gauagcaaaa ucuaucggaa uucaaaaccu aaaaguauuc     2880

gaaaaauaca uaguuagcgc auuaggagaa guaacaaaag cugaauuccg acaaagagaa     2940

gacuucaaaa aauga                                                      2955


<210>  4873
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4873
auguccgacc uaguauuagg acuagacaua ggaauaggcu caguaggagu uggaauuuua       60

aacaaaguca caggcgaaau aauucacaaa aauucacgaa uuuuuccggc agcccaagcc      120

gaaaauaauc uaguaagacg cacuaauaga caaggacgaa gacucgcaag acgaaagaaa      180

cauagacgag uaagauuaaa uagacuauuc gaagaaagug gacucauaac agacuucaca      240

aaaauuucua uaaaucuaaa cccuuaucaa uuacgcguua aagggcuaac ugaugaacua      300

uccaacgaag aauuauuuau agcacuuaaa aauaugguaa aacauagggg cauaaguuau      360

uuagacgaug cauccgacga cggaaacagc uccguaggug acuaugcaca aauaguaaaa      420

gaaaacucua aacaguuaga aacaaaaacc ccuggacaga uucaauuaga aagguaccaa      480

acuuauggcc aauuaagagg cgacuucaca gucgaaaaag acggaaagaa acauagacua      540

aucaauguau uuccaacauc ugcauaucga agugaagcgu ugaggauacu ucaaacucaa      600

caagaguuca acccucaaau aacagaugaa uuuauaaaua gauaucuaga aauacuaaca      660

ggaaaaagaa aauauuacca uggaccagga aaugagaaaa gcagaacuga cuauggacgg      720

uaccgaacaa gcggugaaac auuagauaau auuuucggaa uccuaaucgg aaaaugcaca      780

uucuaccccg augaauuuag agcggcuaaa gcaucauaca cagcacaaga guucaauuua      840

cuaaacgacc uaaacaacuu aacagucccc accgaaacaa agaaacuaag caaagaacag      900

aaaaaccaaa uuaucaauua cguuaaaaau gaaaaagcaa ugggacccgc uaaacuauuu      960

aaauauaucg caaaacuccu uucaugcgau guagcggaca uaaaagggua cagaaucgac     1020

aaaucaggaa aagcagaaau acauacuuuc gaagcauaua gaaaaaugaa aacauuagaa     1080

acacuagaca ucgaacaaau ggacagagaa acacuagaua aauuggcgua uguacuaaca     1140

cucaacacug aaagggaggg aauacaagaa gcucuugaac augaguucgc agaugggagc     1200

uuuucacaaa aacaagucga cgaacuugua caauuccgua aagcaaacag uucaauauuc     1260

ggaaaaggau ggcauaacuu uagcgugaaa uuaaugaugg agcuaauacc agaauuauac     1320

gaaacaagug aagaacaaau gacuauauug acacgucuag gaaaacaaaa gacaacgagu     1380

uccaguaaca aaacuaaaua uauagaugaa aaacuacuaa cagaagaaau uuauaacccc     1440

guuguagcua aauccgucag acaagcuauu aaaaucguaa augcugcaau aaaagaauac     1500

ggagauuucg auaacauagu aauagaaaug gcacgagaaa caaacgaaga cgaugaaaag     1560

aaagcaauac agaaaauuca aaaagcaaac aaagaugaaa aagacgcagc aaugcuaaaa     1620

gccgcaaacc aauacaacgg aaaagcugaa cugccacauu cgguuuucca cggacauaaa     1680

caauuagcaa caaaaauaag auuauggcau caacaaggcg aaagaugcuu auacacagga     1740

aaaacaaucu caauucacga ccugauuaac aacagcaauc aguucgaggu agaucauauc     1800

cuaccccuau cuauaaccuu ugaugacucu cucgccaaca aaguucuagu auaugccacu     1860

gcaaaucaag aaaaagggca aagaacuccc uaucaagcau uagauucgau ggaugaugca     1920

uggucauuua gagaacuaaa agccuucguu agggaaagua aaacauuauc gaauaaaaag     1980

aaagaauauc uuuuaacaga agaggacauc ucaaaauuug acguuagaaa gaaauuuaua     2040

gaacgcaauc uagucgacac ccgauaugcg uccagaguug uauuaaaugc acuccaggaa     2100

cauuuuagag cucauaaaau agauaccaaa guaucaguag uaagaggaca guucacaagc     2160

caauuaagaa gacacugggg uauagagaaa acaagagaca ccuaccacca ccaugcuguc     2220

gacgcccuua uaaucgcagc uucaagccaa cuaaacuuau ggaagaaaca gaaaaauacc     2280

cugguaagcu auagugaaga ucaacuacuc gauauugaaa caggagaauu aauauccgau     2340

gaugaauaca aagaauccgu auuuaaagca ccauaccaac acuuugugga cacauuaaaa     2400

ucaaaagagu ucgaagacuc cauacuuuuc ucauaccaag uagauucaaa auucaaccgu     2460

aaaauaagcg augcaacaau uuaugcuacg cggcaagcaa aaguaggcaa agacaaagca     2520

gacgaaaccu augugcuggg aaaaauaaaa gacauauaua cacaagacgg auacgacgcc     2580

uucaugaaaa uauauaagaa agauaaauca aaauuuuuaa uguauagaca cgacccacaa     2640

accuucgaaa aaguaauaga acccauucug gaaaauuauc caaacaaaca aauaaacgaa     2700

aaaggcaagg aaguucccug uaacccauuc uuaaaauaua aagaagaaca cggauacauu     2760

agaaaauauu caaagaaagg caaugggcca gaaauaaaaa guuuaaaaua uuaugacagu     2820

aaacugggga accacaucga uauaaccccg aaagacucaa auaauaaagu aguccuucaa     2880

uccguaucac ccuggagagc agaugucuau uuuaacaaaa cgaccggaaa auaugaaaua     2940

cuaggcuuaa aauaugcaga cuuacaauuc gaaaaaggua caggaacuua uaaaauauca     3000

caagaaaaau auaacgacau uaagaagaaa gaaggaguug acagcgacuc agaguucaaa     3060

uuuaccuugu auaaaaacga ccuccuucuu gucaaagaca ccgaaacaaa agaacaacaa     3120

cuuuuuagau uccuuucaag aacaaugcca aaacaaaaac acuauguaga auuaaaaccc     3180

uaugauaaac aaaaauucga aggaggggaa gcguuaauaa aaguccucgg uaacguagcc     3240

aacuccggac aguguaagaa aggccuaggc aaaucaaaca uauccauuua caaaguaagg     3300

acagacguuu ugggaaauca gcacaucauu aaaaacgaag gagacaaacc aaaauuagau     3360

uucuga                                                                3366


<210>  4874
<211>  2556
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4874
augacaaaug guaaaauacu uggacucgau auuggcguug cuaguguagg aguaggaauc       60

auagaagcga aaacaggaaa aguaauucau gcaaauagcc gguuauucuc cgcggcuaau      120

gcagaaaaca acgccgaaag aagaggauuc cgcggagcaa gacgacuaac acgaagaaag      180

aaacacagag uaaaaagagu aagagaucuc uucgaaaaau acgauaucuu aacugacuuc      240

agaaauuuaa accuuaaccc auacgaacua cgggucaaag gacuaaccga acaacucaca      300

aacgaggaau uauucgcagc acuucgaacu auagcaaaaa gacgcggcau aaguuacuua      360

gacgaugcag aagaugauuc aacuggguca uccgauuacg ccaaauccau agacgaaaac      420

agaagacuau uaaaaucuau gacccccgga caaauacaac uagaaagacu agaaaaauau      480

ggacaacuca gaggaaauuu cacaguuuau gacgaaaaug gugaagccca cagacuaaua      540

aacguauuca guaccuccga uuauaaaaac gaagcuagaa aaauauuaga aacacaauca      600

aauuauaaua aacaaauaac agacgaguuc auagaagauu acauagaaau acuaacacag      660

aaaagaaaau acuaucacgg accaggaaac gaaaaaucca gaacagauua uggaagauuu      720

agaaccgacg gcacaacucu ugaaaacaua uucggcauac uaauagguaa augcucauuc      780

uauccagaag aauacagagc auccaaagcg agcuacacgg cacaagaguu caauuuccug      840

aacgaucuga acaaucuaaa aguaccgaca gaaacaggaa aauuguccac agaacaaaaa      900

gaauaucuag ucaacuucgc caaaaauuca aaaacacucg gagcaaguaa acuacuaaaa      960

gaaauagcaa aacucgucga uggagaugua aaagacauaa guggauauag ggaagacucu     1020

aaagguaaac cagaucuaca uaccuucgaa ccauauagga aacuuaaauu uaaccuaaca     1080

acaguagaca uagacaauuu aaguagagau auauuagaua aacuugcaga uauccucaca     1140

cuaaacacag aacgagaagg aauagaagac gcaaucaauc aaaaucuacc agaacaauuc     1200

accaaagaac aaaucucaga aauagugcaa auacgaaaaa gucaaagcuc agcauucaac     1260

aaaggauggc acucuuucuc agccaaacua augaacgaac uaauaccaga auuauacgca     1320

acaagcgagg aacaaaugac aauuuuaaca aggcucgaaa aauucaaagc aacaaagaaa     1380

ucaaguaaaa auaccaaaac aauagaugaa aaagaaauaa cugacgaaau cuauaaucca     1440

guaguggcaa aaucaguaag acaaacaauc aaaauaauaa acgcagcagu aaagaaauau     1500

ggugauuuug acaaaauagu aauagaaaug ccaagagaca aaaaugcaga ugacgagaag     1560

aaauucauag auaagaaaaa uaaagaaaau aagaaagaaa aagaugacuc acugaaaaga     1620

gcggccuauc uauacaacgg aacugacaaa cuaccugacg acguauucca cggaaacaaa     1680

cagcuagcca caaaaaucag guugugguau caacaaggag aaagaugccu auacagcgga     1740

aaaccaauac uaauacaaga ucuaguucac aacucaaaca acuucgaaau cgaucauaua     1800

cuacccuuau cauuaaguuu cgacgacagc uuagcaaaca aaguauuagu auaugcaugg     1860

gccaaccaag agaaagaaca gaaaacgccc uaccaaguua uagauucaau ggacgcagca     1920

ugguccuuua gagaaaugaa agacuacgua cuaaaacaaa aagguauagg aaagaagaaa     1980

agagaauauc uacuaacuac agaaaacaua gauaaaauag aaguaaagaa gaaauuuauu     2040

gaaagaaauc uaguagacac uagauaugca aguagaguag uacuaaacag uuuacaaaca     2100

gcauuaaaag aauuaggaaa agacacaaaa guuagcguag ucagaggcca auucacaucc     2160

caacuacgaa ggaaauggaa aauagauaaa agccgagaaa cauaccacca ccacgccgua     2220

gacgcccuaa uaauagcagc aaguucccaa cuaaaauuau ggcaaaaaca cgaaaauuua     2280

auguuugaaa auuacggaga aaaucaagua guaaauaaag aaacagguga aauacucagu     2340

auaagcgacg acgaauauaa agaaucggua uuucaaccac ccuaucaagg guucgugaau     2400

acaaucucgu caaaagcguu ugaagacgaa auuuuauuca guuaccaagu ugacucaaaa     2460

uuuaaucgaa aagucucaga cgccacgaua uauucgacaa gaaaagcaaa acuaggaaaa     2520

gauaagaaag aagaaaccua cguacuugga aaauga                               2556


<210>  4875
<211>  2598
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4875
augagacaca aaaucggacu agacaucgga auaaccagug uuggcugggc aguaauaaau       60

cuagauauac cucguaucga agaucuagga guacgaauuu ucgacagggc cgaaaauccu      120

cagaccggag aaucauuagc acuacccaga agacuugcaa ggucugccag aagaagacua      180

agacgaagaa aacacagacu ggagagaaua cgaagacucu uaauaagaga agguauauua      240

acaaaagaag aacuagauaa auuauuugaa gaaaaacacg aaauagacgu auggcaauua      300

agaguagaag cauuggauag gaaacuaaac aacgaugaau uagcacgugu ucuacuacac      360

uuagcaaaac gaagaggauu uaaaucaaau agaaaaucag aaagaaauaa caaggagaac      420

agcacuaugc ugaaacacau agaagaaaau agagcauugu uauccgguua cagaacagua      480

gcagaaauga uaguaaaaga cccaaaauuu gcauuccaca aaagaaauaa aggagaaaac      540

uauaccaaua cuauagcgag agaugaccua gaaagagaaa ucaaacuaau auuuacgaaa      600

caaagagaau auggaaacau uguguguaca gaaacauuug aaaacgaaua uauuaugauu      660

ugggcaagcc aacgaccauu ugcaucaaaa aacgauauag aaaaaaaagu agguuuuugu      720

acuuuugaac caaaagaaaa aagagcucca aaagcaacau auaccuucca aaguuuuguc      780

gcaugggagc auaucaauaa acuccgauug gucuauccaa ccggaacacg aggacuaacc      840

gacgaagaaa gacgauuguu auauaaacaa gccuuccaca aaaauaaaau aacuuaucau      900

gauauaagaa cuuuacuuca uuuaccagau gacacauacu uuaaaggaau cguuuaugac      960

agaggagccc cacuaaaaca aucagaaaca auaagauuuu uagaacucga cgcuuaucau     1020

caaauaagaa aagcaguaga caaaguauau ggcaagggaa aaaguucauc auuccuucca     1080

aucgacuuug acacauucgg auaugcacua acacuauuca aagacgacgc ugacauaaga     1140

ucauauuuaa gaaaugaaua ugaacagaac gguaaaagaa ugccaaaucu agcaaacaag     1200

gucuacgaua augagccuau cgaggaacuc uuaaaucuau cauuuacaaa auucggacac     1260

cuaucucuua aagcauuacg caacauacuu ccauacaugg aacaaggaga aguuuauuca     1320

guagcuugug aaagggcagg guacaccuuc acaggaccaa aaaaaaaaca aaaaacacua     1380

cuacuaccaa acauaccacc aauagcaaac cccgucguaa ugcgcgcacu cacacaagca     1440

agaaaaguug uaaaugcaau aaucaaaaaa uacgguucgc caguaucgau acacauagaa     1500

cucgccagag aucuauccca aacauucgac gaaagaagaa aaacaaaacg agaacaagau     1560

gaaaaucgua aaaaaaacga aacagccaua aggcaguuga uggaauacgg acuaaccauu     1620

aacccuaccg gacaugauau uguaaaauuu aaacuauggu ccgagcaaaa uggacggugu     1680

gccuauucuc uacaaccaau agaaauagaa agacuucuag aaccaggaua cguugaagua     1740

gaccacguaa uaccauauag ccgcagucua gacgacucuu auacgaacaa aguacuugug     1800

cuaacaaaag aaaauagaga aaaagguaac cgaauaccag cagaauauuu aggagucggg     1860

acagaaagau ggcaacaauu uaaaacauuu guacuaacaa acaaacaauu cucuaaaaaa     1920

aaaagagaua gauuacuaag auugcacuac gacgagaaug aagaagcuga auggaaaaac     1980

cgaaauuuaa augauacuag auauauuuca agauuuuucg ccaauuuuau ccgcgaacac     2040

cuaaaauuug cagaaucaga ugauaaacag aaagucuaca caguaaaugg aagagugaca     2100

gcccacuuga ggagccgaug ggauuuuaau aaaaaccgag aagaaaguga cuuacaccac     2160

gcaguugacg cugcaauagu cgcaugcacu ucacccuccg acauagcaag aguaacagca     2220

uuuuaucaac gcagggagca augcaaagag agugccaaaa aagcggaacc ccacuucccc     2280

caacccuggc cucauuuugc ugaugaauua cgagccagac uaucgaaaaa ucccaaagag     2340

ucaauaaaag cuuuaaacuu aggaaacuau gaugaccaaa aacuagaauc ucuacaacca     2400

guguuuguau cacgaaugcc aaaacgcuca guuacuggag cagcacauca ggaaacauua     2460

cgcagauaua uuggaaucga cgaaagaucc gguaaaauac aaaccguagu caaaacaaaa     2520

cuuucagaaa uuaaauuaga ugaaacagga cacuucccaa uguauggcaa agaaucagac     2580

cccccagcaa gugcauga                                                   2598


<210>  4876
<211>  2886
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4876
auggcaagaa uacuugcuuu ugauauaggu auuagcagca uaggcugggc cuucucugaa       60

aaugacgaac ucaaagacug uggcgucaga aucuucacaa aagcugaaaa uccaaaaaca      120

ggcgaaucac uagcacuucc ccgaagacua gcaagaucag cacgaaaaag acuugcucgc      180

cgaaaagcaa gauuaaacca ucuaaaacau cuaauagcaa acgaauucaa acuaaacuac      240

gaagacuacc aaucauuuga cgaaucuuua gcaaaagcau auaaaggcuc acuaaucagu      300

ccuuacgaac ucagauucag agcacucaac gaacuacuaa guaaacagga cuucgcaaga      360

guaauacugc acauagcaaa aagaagagga uacgaugaca uaaaaaacaa uggagacgaa      420

gaaaaaggaa aaauauuaaa ggcaauaaaa caaaacgaag aaaaauuagu gaacuaucaa      480

agcguaggag aauacuuaua caaagaauac uuucaaaaau ucaaagaaaa uucuaaagaa      540

uucauuaaug uaagaaacaa aaaagaaagu uacgaaagau gcauaacaca aaguuuccuu      600

aaauaugaac uaaaacuaau cuuccaaaaa caaagagaau ucggauucuc cuuuuccaaa      660

aaauucgaag aagaaguacu auccguagca uuuuauaaac gagcacuaaa agauuucagu      720

caccuugucg gaaauugcuc cuucuucagc gacgaaaaaa gagcaccaaa aaacucccca      780

cucgcauuua uguucgucgc acuaacacga aucauaaacu uacuaaauaa ccuaaaaaau      840

acagaaggua uccuuuacac aaaggaugac uuaaacgcac uucugaacga gguacugaaa      900

aauggaacuc uaacauacaa acaaacaaaa aaauuauuag gauuaaguga cgacuaugaa      960

uucaaaggcg aaaaaggcac auauuuuaua gaauucaaaa aauacaagga auucauuaaa     1020

gcauuagggg aacauaaccu cucacaagac gaccuaaacg aaauagcuaa agauauaacu     1080

uugauaaagg augaaauaaa acuaaaaaaa gccuuagcaa aauacgaccu aaaccaaaau     1140

caaauagacu cauuaucaaa acuagaauuc aaagaccacc ucaacauagg guucaaagca     1200

cuaaaacuaa ucacccccuu aauguuagaa gguaaaaaau augaugaagc auguaaugaa     1260

cuaaauuuaa aaguugccau caaugaggac aaaaaggacu uucuacccgc cuuuaaugaa     1320

acuuauuaca aagaugaagu cacaaacccc guagucuuac gagcaauaaa agaauacagg     1380

aagguacuua acgcauuauu aaaaaaauac ggaaaaguac acaaaauuaa cauagaacua     1440

gccagagaag uugguaaaaa ccacagccaa agagcaaaga uugaaaaaga acaaaacgaa     1500

aacuacaaag caaaaaaaga ugcagagcua gaaugcgaaa aacucggacu aaaaauaaac     1560

uccaaaaaca uauuaaaacu ccgguuauuu aaagaacaga aagaauucug ugcauacuca     1620

ggugaaaaga uaaaacugag ugaccuacag gacgagaaaa ugcuagagau cgaccacaua     1680

uacccauauu ccagaagcuu ugaugauagc uauaugaaca aagugcucgu uuucacaaaa     1740

caaaaccagg aaaagcuaaa uaaaacucca uucgaagcau ucggaaacga caacguaaaa     1800

uggcaaaaaa uagaaguuuu agcuaaaaac cuaccagaaa aaaaacaaaa aagaauauua     1860

gacaaaaacu acaaagacaa agaacaaaaa gacuuuaagg auaggaaccu uaacgauacc     1920

agauacauag cucgauuagu auuaaacuac acaaaagacu accuagacuu cuuaccucuc     1980

ucagacgacg aaaacaccaa acucaaagga ucaaaaguuc auguagaagc aaaaucagga     2040

auguuaacaa gcgcacuaag gcauacaugg gguuucagua cuaaagacag gaauaaccac     2100

cuacaccacg caauagacgc agcaauuaua gccuacgcaa auaacagcau aguaaaagac     2160

uucagcgacu uuaaaaaaga acaagaaucu aacucagccg aacucuaugc uaaaaaaaua     2220

uccgaauuag acuacaaaaa uaaacgcaaa uuuuucgaac cuuuuucagg auucagacaa     2280

aaaguguuag auaaaauaga ugaaauauuu guaucaaaac cagaacgcaa aaaaccuagc     2340

ggcgcucuac augaagaaac auuuagaaaa gaagaagaau uuuaucaaag cuauggaggc     2400

aaagaaggag uacuaaaagc acucgaacua ggaaaaauuc gaaagguuaa uggaaagauu     2460

guaaaaaacg gagacauguu ccguguagac auauuuaaac acaagaaaac caacaaauuc     2520

uaugccgucc cuauauacac aauggauuuu gcucuuaaag uacuaccaaa caaagccgua     2580

gcuagaucaa aaaaagggga aaucaaagac uggauccuaa uggacgaaaa uuaugaguuu     2640

uguuucagcc uuuacaaaga cagucuaauc cuuauacaga ccaaagacau gcaagaacca     2700

gaacuaguau acuucaaugc cuuuaccagu agcacgguau ccuuaauagu aaguaaacau     2760

gauaauaagu ucgaaacacu caguaaaaac cuaaaaguau ucgaaaagua uauaguuagc     2820

gcacucggag aagugacuaa agcagaguuc cgacaaagag aagauuucaa aaacgaacua     2880

cgcuga                                                                2886


<210>  4877
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4877
auggcaaaaa uacuagcauu cgauauaggc auaucgagca uaggcugggc cuucucagaa       60

aacgaugaac ugaaagacug ugguguuaga auauuuacca aagcggaaaa cccaaaaaca      120

ggagaauccu uagcucuacc aagaagaaua gcaaggucug caagaaagcg auuagcuaga      180

cguaaagcaa gauuaaacca cuuaaaacac cuaauagcaa acgaauuuaa acuaaauuau      240

gaagacuacc aauccuucga cgaaauccuc gcaaaagcuu acaaaggauc cuuaauaagu      300

ccuuaugaau uaagguuuag agcacucaac gaauuacugu cuaaacaaga cuuugcacga      360

guaauucuac acauagccaa acguagaggc uaugacgaua uaaaaaacaa cggagacgaa      420

gagaagggaa aaauacuaaa agcuauaaaa caaaacgaag aaaaacuggc caacuaccaa      480

ucuguaggug aauacuuaua caaagaauac uuucaaaaau uuaaagaaaa cucaaaagaa      540

uuuaucaacg uaagaaacaa aaaagaauca uacgaaagau gcauagcaca auccuuccua      600

aaagacgaac uuaaguuaau auucaaaaaa caaagagagu ucggauucuc auucagcaaa      660

aaguucgaag aagaaguccu auccguagca uucuacaaaa gagcacuaaa ggacuucuca      720

cauuuaguug guaauugcag uuucuucacg gaugaaaaaa gggcaccaaa aaacagccca      780

cuagcauuca uguucguagc acuuacaaga auaauuaauc uuuuaaacaa ccuaaaaaau      840

acacuuuaua cgaaagacga cuugaaugca uuauuaaacg aaguccuaaa aaacggaacc      900

uuaaccuaua aacaaacaaa aaaacuacuc gguuuaucag acgauuacga auucaaagga      960

gaaaaaggua auuacuucau agaauuuaag aaauacaaag aauucauaaa agcacucgga     1020

gaacauucac uaucacaaga cgaucuaaac gaaauugcaa aagacauuac acuaauuaag     1080

gaugagauua aauuaaaaaa ggcccuugca aaauaugauu uaaacgaaaa ucaaauugau     1140

aaccuaagca aauuagaauu uaaagaucac cuaaacauau cuuucaaagc gcuaaaguua     1200

auaaccccac uaauguuaga aggcaaaaag uacgaugaag caugcaauga acuaaccuua     1260

aaaguugcaa ucaaugaaga caaaaaagac uuucuaccag cauucaauga aaccuacuac     1320

aaagaugaag uaacaaaccc cgucgucuua cgagcaauca aagaauaucg aaaaguacua     1380

aacgcacuau uaaaaaaaua ugggaaagua cacaaaauua auauagaauu agcacgcgaa     1440

guagggaaaa accauucaca aagagccaaa auagaaaaag agcaaaacga aaauuauaaa     1500

gcaaaaaaag acgcagaacu cgaaugcgaa aaacuaggac uaaaaauaaa uaguaaaaau     1560

auuuuaaaac uaagacuauu caaagaacaa aaagaauuuu gcguauauag cggagaaaaa     1620

auaaaauuaa gugaucuaca agaugaaaaa augcuagaga ucgaccacau uuaccccuac     1680

agucgaucau ucgacgauuc cuauaugaau aagguguuag uauucaccaa acaaaaccaa     1740

gaaaaacuca accaaacacc auucgaagca uucggaaaug auucugcuaa auggcagaaa     1800

aucgaaguau uagcuaaaaa ccuaccgacc aaaaaacaaa aacgaauacu agacaaaaac     1860

uacaaagaca aagaacagaa agacuucaag gauagaaauc uuaacgauac ccgauacauc     1920

gcaagauuag uuuuaaacua uacaaaagac uaccuagacu uuuugccguu auccgacgau     1980

gaaaacacaa aacucaauga uacacaaaag gguucaaaag uacacguaga ggcaaaaucc     2040

ggaaugcuaa ccagugcguu aagacacacc uggggauucu cuacaaaaga ucggaacaac     2100

caccuccacc acgcaauuga cgcuguaaua auagcauacg caaacaacag cauaguuaaa     2160

gcuuuuucag auuucaaaaa agaacaagaa ucaaacacug ccgaauuaua ugcaaaaaaa     2220

auaagugaac uagauuacaa aaacaaaaga aaauucuucg aacccuucuc aggauuuaga     2280

caaaaaguac uagauaaaau agacgaaauc uucguaucaa aaccagaaag aaaaaagcca     2340

ucaggcgcac uacacgaaga aacauucaga aaagaagaag aauucuauca aaguuacggu     2400

ggcaaagaag gaguucuaaa agcucuagaa cugggaaaaa uacgaaaagu uaacggaaaa     2460

auaguaaaaa acggugauau guuccgaguu gacaucuuca aacacaaaaa aacgaacaaa     2520

uucuacgcag ugccaauaua cacuauggac uucgcacuaa agguauuacc gaacaaagca     2580

gucgcuagga gcaagaaagg agaaauaaaa gacuggauac uaauggauga aaacuacgaa     2640

uucugcuucu cacuauauaa agacucacua auacucauac aaacuaaaga caugcaagaa     2700

ccagaauuug uauauuacaa cgcauuuaca ucauccacag ucagccuaau aguaucaaaa     2760

cacgacaaca aauucgagac ccucagcaaa aaccaaaaaa uuuuauuuaa aaacgcaaac     2820

gaaaaagaag ucauagccaa auccauaggc auacaaaacc ugaaaguguu ugaaaaauac     2880

auagucucag cacuaggaga agucacaaaa gcagaauuuc gacaaagaga agaauucaaa     2940

aacgaacugc gauga                                                      2955


<210>  4878
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4878
auggcacgaa uacuagccuu cgacaucgga auaucaucaa uuggaugggc auucucagaa       60

aaugacgaac uaaaagacug cggcgugaga auauucacua aagcugagaa uccaaaaaca      120

ggagaaaguc uagcauuacc cagaagacua gccagaucag caagaaaaag acuugcaaga      180

agaaaagcaa gauugaacag auuaaaacau uuaauagcaa acgaauuuaa auuaaacuac      240

gaagacuauc agucauucga cgaaagccug gcuaaggcau auaaaggauc acuaauuagc      300

ccuuacgaac uacguuucag agccuugaac gaguuacucu caaaacaaga uuuugcaaga      360

guaauacuac acauagcaaa aagaagggga uaugacgaua uaaaaaauuc agacgacaaa      420

gaaaaaggcg ccauccuuaa agccaucaaa caaaaugaag aaaagcuagc aaacuaccaa      480

ucagucggag aauaccucua uaaagaauac uuccaaaaau ucaaagagaa cucaaaagag      540

uucaccaaug uacgaaacaa aaaagaaagc uacgaaagau guauagccca gucauuccuc      600

aaagacgaau uaaaguuaau auucaaaaaa caaagagaau ucggauuuag cuucucuaaa      660

aaauuugaag aagaaguccu aagcguggcc uucuauaaac gagcacuaaa agauuucuca      720

caccuaguag guaacugcuc cuucuuuacu gacgaaaaga gagcgccaaa aaacucgccg      780

uuagcauuca uguucguagc auuaacaaga aucaucaacc uacugaacaa cuuaaaaaau      840

acagaaggua uccuauacac caaagaugac cuaaacgccc uacucaacga auuuuuaaaa      900

aacggcacuc uaaccuauaa acaaacuaaa aaauuacucg gauuaaguga ugacuacgaa      960

uucaaaggag aaaagggaac uuacuucaua gaauuuaaaa aauacaaaga guucauaaaa     1020

gcauuaggcg aacauaaucu aagucaagac gaccuuaaug aaauagcuaa ggauauaacu     1080

cuaaucaaag acgaaaucaa acucaaaaaa gccuuagcua aauaugaccu uaaccaaaac     1140

caaauagaca gcuuaaguaa auuagaauuu aaagaccauu uaaauauauc cuuuaaggca     1200

cuaaaauuaa uaacaccccu uaugcuagag gggaaaaaau acgaugaagc gugcaaugaa     1260

cuuaaucuca aaguagcuau aaaugaagau aaaaaagauu uccuaccagc auucaacgaa     1320

acauauuaua aagaugaagu aacaaaccca guaguauuga gagcaauaaa agaauaucga     1380

aaaguccuaa acgcacuuuu aaaaaaguac ggcaaaguac auaaaaucaa cauugaacuc     1440

gccagagaag uuggaaaaaa cuauagucaa agagccaaaa uagaaaaaga acaaaaugaa     1500

aacuauaaag cuaaaaaaga cgccgagcuu gagugugaga aacucggacu aaagauaaau     1560

ucaaagaaca uauuaaaauu acguuuauuu aaagaacaaa aagaauucug cgcguauagc     1620

ggcgaaaaaa uaaagaucuc ugaucuacaa gaugaaaaaa uguuagaaau agaccauaua     1680

uacccuuaca guagguccuu cgacgacagc uacaugaaca aaguguuagu auuuacaaaa     1740

cagaaccaag aaaaauuaaa ucaaaccccg uucgaagccu uuggaaacga cagcgcaaaa     1800

uggcaaaaaa uagaagucuu agcaaaaaac cucccaacua aaaaacaaaa aagaauccua     1860

gauaaaaauu auaaagacaa ggaacaaaaa gauuuuaaag acaggaaccu aaacgauaca     1920

agauauauag caagacuagu ucucaauuau accaaagacu auuuagacuu uuuaccgcuu     1980

agugacgaug aaaacaccaa guuaaacgau acucauaaag gaucgaaagu ucacguagaa     2040

gccaaaagug gaauguugac aagcgcguua agacauacau ggggcuuuuc aacuaaagau     2100

cgaaauaacc acuuaagaca ugcaauagac gcgguaauaa uagcauaugc caacaacucu     2160

auagucaaag ccuucaguga uuuuaaaaaa gagcaagaau caaauuccgc agaacuauac     2220

gcgaaaaaaa uuagugaauu agacuauaaa aacaaaagaa aauuuuuuga accguuuuca     2280

ggauuuagac aaaaaguacu ugacaaaauc gacgaaaucu uuguaucgaa acccgagaga     2340

aaaaaaccau ccggggcucu acaugaggaa acuuucagaa aagaagaaga guucuaccaa     2400

ucuuacggag gaaaagaagg aguacucaaa gcacuagaau uaggaaaaau acgaaaaguc     2460

aacggaaaaa ucguaaaaaa cggcgauaug uuuagaguag acaucuuuaa acacaaaaaa     2520

accaacaaau ucuaugccgu gcccauauac acaauggacu uugcuuuaaa aguacuacca     2580

aacaaagccg uugcuagaag caaaaagggu gaaauaaaag auuggauccu aauggacgaa     2640

aacuaugaau ucugcuucuc ucuauacaaa gacucucuua uccuaauaca aacaaaagac     2700

augcaagaac cagaguuugu auacuacaac gcauucacaa guucaacagu aagucuaaua     2760

guuucaaaac augacaauaa auucgaaacc cuaucaaaaa accaaaaaau acuuuucaaa     2820

aacgcaaacg aaaaagaagu gauugcaaaa ucaaucggaa uacaaaaccu caaaguauuc     2880

gaaaaauaca uuguaucagc ucucggagaa guaacaaaag ccgaauucag acaaagggaa     2940

gacuuuaaaa aauga                                                      2955


<210>  4879
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4879
auggcacgaa uacucgcguu ugauauuggu auuagcucca uuggaugggc auucucagaa       60

aaugacgaac ucaaagacug cggcgucagg aucuucacua aaguagagaa cccaaaaacc      120

ggagaaagcc uugcacuacc uagacgacua gcacguagug caagaaaaag acucgcacgg      180

cgaaaagcaa gacuaaauca ucuaaaacac uuaauagcaa acgaauuuaa auuaaauuau      240

gaagacuacc aguccuucga ugaaucacua gccaaagcau acaaaggaag ccucauaagu      300

cccuacgaac ucagauuccg ggcauuaaau gaacuucuau caaaacaaga uuucgcacga      360

gucauauuac acauugcaaa acgcagagga uaugacgaca uaaaaaauuc agacgacaaa      420

gaaaaaggag caauacuaaa agcaaucaaa caaaaugaag aaaaacuagc aaacuaccaa      480

ucaguaggcg aauaccuaua uaaagaauac uuucaaaaau ucaaggaaaa cagcaaagaa      540

uucacuaacg uacggaauaa aaaagaauca uaugaaagau guauagcaca aucauuucua      600

aaagacgagc uaaaacuuau auucaaaaaa caacgagaau uuggauucuc auuuagcaaa      660

aaauuugaag aggaagugcu gucaguugca uucuauaaaa gagcucuaaa agacuuuagc      720

cauuugguag gaaacuguag cuucuucacc gacgaaaaac gagcaccuaa aaauagccca      780

uuagcguuca uguucguaac ucuuaccaga auaauaaacc ucuuaaauaa ucugaaaaau      840

acagaaggaa uacuauacac caaagacgac cuaaaugcuc uacucaauga aguuuuaaaa      900

aacggaacgu uaacauacaa acaaacaaaa aaacuacuag gacuaagcga cgacuacgaa      960

uucaaaggag aaaaaggaac auacuuuauc gaauucaaaa aauacaaaga auuuauaaaa     1020

gcgcuuggcg aacacaaucu aagccaagau gaccuuaacg aaaucgcaaa agauauuacg     1080

cuaauuaaag acgaaauaaa acuaaaaaaa gcauuagcaa aauaugaccu aaaccaaaau     1140

caaauagauu cacuuaguaa acuagaguuc aaagaucacc uaaacauuuc auucaaagcu     1200

cuaaagcuaa uaacaccauu aaugcuagag gggaaaaaau acgacgaagc augcaaugaa     1260

uugaaccuca aaguagccau caacgaagac aaaaaagacu uucuaccagc auucaacgaa     1320

accuacuaca aagacgaagu aaccaauccu guaguucuaa gagcaauaaa agaauacaga     1380

aaaguccuaa acgcgcuccu aaaaaaauac ggaaaaguac auaaaauuaa cauagaauua     1440

gcaagagaag uaggaaaaaa ucacucacaa cgagcuaaaa ucgaaaaaga acaaaacgaa     1500

aauuacaaag ccaaaaaaga cgcugaguua gaaugugaaa aauuagguuu aaaaauaaau     1560

ucaaaaaaca ucuuaaaacu acgccuauuc aaagaacaga aagaguuuug cgcuuacucg     1620

ggagaaaaaa ucaaaaucag ugacuuacaa gaugaaaaaa uguuagaaau ugaucacaua     1680

uauccauauu cccguaguuu ugacgacucu uacaugaaca aaguacuagu guuuacaaaa     1740

caaaaccagg aaaaacuaaa uaaaacacca uucgaagcau ucggaaauga uuccgcaaag     1800

uggcaaaaaa uugaaguacu agcuaaaaau uuacccacaa aaaaacaaaa acgcauacua     1860

gacaaaaauu auaaagacaa agaacaaaaa gauuuuaaag accggaaucu aaacgauaca     1920

agauacaucg caagacuagu acucaacuac acaaaggauu aucuugauuu ucuaccucuc     1980

ucagaugacg aaaacacaaa auuaaacgau acacaaaaag gaucaaaagu gcacguagaa     2040

gcaaaaucug gcaugcuaac aucagcauua cgccauaccu ggggauuuuc aacuaaggac     2100

agaaauaacc acuuacacca ugcaauagau gcagucauca uagcuuacgc gaauaauuca     2160

aucgucaaag cauucuccga cuuuaaaaaa gaacaagaaa guaacucagc agagcuuuac     2220

gcaaaaaaaa uuucggaauu agacuacaaa aauaaacgaa aauuuuucga accauucagc     2280

gguuuuagac aaaaaguauu agacaagaua gaugaaauau uugucucgaa accagaacga     2340

aaaaaaccaa guggagccuu acaugaagaa acuuuccgaa aagaagaaga auucuaccaa     2400

aguuauggag gcaaagaggg cgucuuaaaa gcauuagaau uaggaaaaau acggaaagua     2460

aauggaaaaa uagucaaaaa cggcgauaug uuuagaguag acauauucaa acacaaaaag     2520

acaaauaaau uuuaugcagu uccaauauac accauggacu ucgcucuaaa aguuuuaccc     2580

aauaaagcgg uagcaagauc aaaaaaagga gagauaaaag auuggauacu aauggaugaa     2640

aauuacgaau uuugcuucag ccucuacaag gacaguuuaa uacucaucca aaccaaagac     2700

augcaagaac cagaauucgu uuauuacaau gcguuuacau caucaacagu aagccuaauu     2760

guaucaaaac acgacaauaa auucgaaaca uuaucaaaaa accaaaaaau auuauuuaaa     2820

aacgcaaaug aaaaagaagu aauagcuaaa ucaaucggca uacaaaauuu gaaaguauuc     2880

gaaaaauaua uuguaucagc acuuggugaa guaaccaaag ccgaauucag acaaagggaa     2940

gacuuuaaga aauga                                                      2955


<210>  4880
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4880
auggcaagaa uacuagcguu ugacaucgga auaucaucaa uaggaugggc auucucggaa       60

aaugacgaac uaaaagacug uggaguaaga auauuuacaa aagcggaaaa ccccaaaacu      120

ggugaaucau uagcacuucc cagaagauua gcaagaagug cuagaaaacg acuagcacga      180

agaaaagcaa gauuaaauca ccuaaaacau cuaaucgcaa acgaauucaa acucaacuau      240

gaagacuauc aaucauuuga cgaaucacua gccaaagcau acaaagguuc guuaauaagc      300

cccuaugaac ucagauucag agcccucaac gaacuauuaa guaaacaaga cuucgcucgu      360

guaauacucc acauagcuaa aagaagagga uacgaugaca uuaagaacuc agacgacaaa      420

gaaaaaggag cuauacuaaa agccauaaaa cagaacgaag aaaagcuagc aaauuaccaa      480

ucaguaggcg aauaccuuua caaagaauac uuccaaaaau ucaaagaaaa cuccaaagaa      540

uuuacgaacg ugagaaacaa aaaagaaucc uacgaaagau gcauagcaca aagcuuuuua      600

aaagacgaac uaaaacuaau auuuaaaaag caacgagaau uuggauuuuc auuuagcaaa      660

aaguucgaag aagaaguacu aagcguugca uuuuacaaac gagcacucaa agacuuuuca      720

caucucgucg gcaauuguuc guuuuucaca gacgaaaaga gagcacccaa aaacucacca      780

cucgcuuuca uguuuguagc guuaacaaga auaauaaauc uccuaaauaa ccucaaaaac      840

acggaaggua uauuauauac aaaagacgau cucaacgccc uacucaacga aguucuuaaa      900

aaugguacac uaacuuacaa acaaacaaaa aaguuacuag gacuaagcga cgauuaugaa      960

uucaaaggcg aaaaagguac auacuucauc gaauucaaaa aauacaaaga auucauaaaa     1020

gcuuuaggcg aucauucacu aucacaggac gaccuaaaug aaauagccaa agauauaacg     1080

uuaauaaaag acgaaauaaa acugaaaaaa gcgcucgcaa aauaugaccu uaaccaaaac     1140

cagauagaca gccucaguaa auuagaauuc aaagaccacu uaaauauuuc auucaaagcc     1200

uuaaaauuaa ucacaccauu aaugcuagaa gguaaaaaau augacgaagc cuguaacgaa     1260

cuaaauuuaa aaguggcaau aaacgaagac aaaaaagacu ucuuacccgc auuuaacgag     1320

acauauuaua aagacgaagu uacaaaccca guaguacucc gagcuauaaa agaauauaga     1380

aaaauauuaa acgcacuauu aaaaaaauau ggaaaaguac acaaaauaaa caucgaauua     1440

gcgagagaag ugggaaaaaa ccauagucaa agagcaaaaa ucgaaaaaga gcaaaacgaa     1500

aauuauaaag caaaaaaaga cgcagaaaua gaaugugaaa aacuggguuu gaaaauaaac     1560

uccaaaaaca uacuaaaauu aagauuauuc aaagagcaaa aagaauucug cgcauauuca     1620

ggcgaaaaaa uaaaauuaag cgaucuucaa gaugaaaaaa ugcucgaaau agaccacauc     1680

uacccuuaca guagaucauu cgacgacagu uauaugaaca aaguucuagu auuuaccaaa     1740

caaaaccaag aaaaacuaaa caagacacca uuugaagccu ucggaaauga cagcacaaaa     1800

uggcaaaaaa uagaggucuu ggccaaaaac cuaccagaaa aaaaacaaaa gcguauacua     1860

gacaaaaauu acaaggacaa agagcaaaaa gauuucaaag auagaaauuu aaaugauaca     1920

agauacauag caagauuagu acuuaacuac acaaaggacu aucuagacuu ucugccacuu     1980

agcgacgacg aaaacaccaa acugaaugau acacaaaaag guucaaaagu gcacguagaa     2040

gcaaaaucgg gcauguuaac aucagcacuc agacacaccu ggggcuuuuc cgcaaaagac     2100

aggaauaauc acuuacacca ugcaauagau gcagcuauca ucgccuaugc aaauaacucc     2160

aucguaaaag cauuuagcga uuucaaaaaa gaacaagaaa guaauucggu agaacuuuac     2220

gcaaaaaaga uaucagaacu agauuauaaa aacaaaagaa aauucuucga acccuucuca     2280

ggcuuuagac aaaaaguacu cgacaaaaua gacgaaaucu uuguaucaaa gccagaacga     2340

aaaaaaccaa guggagcuuu acaugaagaa acauucagaa aagaggaaga auucuaccaa     2400

aguuacggcg gaaaagaagg cguauuaaaa gcacuagaau uaggaaaaau ucgaaaagua     2460

aauggcaaaa uaguaaaaaa cggugacaug uuuagaguag auaucuuuaa acacaaaaag     2520

acaaacaaau ucuacgcagu accaauuuac acgauggacu ucgcauuaaa aguacuacca     2580

aauaaggcag ucgcccgcuc aaaaaaaggc gaaauaaaag acuggaucuu aauggacgaa     2640

aacuaugaau ucuguuucuc auuauacaaa gacucccuga uacucauaca aacaaaagac     2700

augcaggaac cagagcuugu cuacuucaac gcuuuuacuu ccuccaccgu uagccuaauc     2760

guaucuaaac acgacaacaa auucgaaaca cugaguaaaa accaaaaaau acuguucaaa     2820

aaugccaacg aaaaagaggu cauagcuaaa ucaauaggua uacaaaaucu uaaaguguuu     2880

gaaaaauaca uagucucagc guuaggagaa gucacaaaag cagaauucag acaaagagaa     2940

gacuuuaaaa aauga                                                      2955


<210>  4881
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4881
auggcaagga uacuggccuu cgacauaggu aucucgucua uaggaugggc cuuuucagaa       60

aaugaugaac uaaaagacug cggaguaaga auauuuacca aaguagaaaa ucccaaaaca      120

ggagagucac uagccuuacc acgacgucuu gcgagaagcg caagaaaaag auuagcgagg      180

aggaaagcac gacuaaauca ccuuaaacau uuaauagcua acgaauuuaa auuaaacuac      240

gaagacuacc aaaguuuuga ugaaaguuug gcuaaagccu acaaaggaag ccucaucagu      300

cccuacgaac uaagauuuag agcccugaau gaacuccuua guaaacaaga cuuugcacga      360

guaauauuac acauagcaaa acguagaggu uaugaugaca uaaaaaacag cgaugacaaa      420

gaaaaaggag ccauacugaa agcgauaaaa caaaacgaag aaaaauuagc aaacuaucag      480

ucggucggug aauaccucua caaagaauau uuucaaaaau ucaaagaaaa uagcaaagaa      540

uucaccaaug uaagaaacaa aaaagaaagc uacgaacgau gcaucgcaca aagcuuccua      600

aaagaugaau uaaagcuaau auucaagaaa caaagagaau ucggauuuuc cuucagcaaa      660

aaauucgaag aagaaguacu aucagucgcu uucuacaaac gagcacuaaa agacuucagc      720

cauuuaguag gaaacuguag cuucuucaca gaugaaaaac gggcaccaaa aaacagcccc      780

cuagccuuca uguuuguugc gcuaacacga auaauaaauc ucuuaaacaa ucucaaaaac      840

acagaaggaa uacuauauac aaaagaugac cuuaaugcau uacugaacga agucuuaaaa      900

aacgguacac uuacuuauaa acaaacuaaa aaacuacucg gccuaaguga cgacuaugaa      960

uucaaaggag aaaagggcac auacuuuaua gaauucaaaa aauauaaaga auuuauaaaa     1020

gcauuaggcg aacauaaucu aucccaagac gaucucaacg aaauagcuaa agauaucacu     1080

uuaauaaaag augaaauaaa auuaaaaaaa gccuuagcaa aauaugaccu aaaccaaaau     1140

caaauagauu cacuaucuaa auuagaauuu aaggaccauu uaaauaucuc auuuaaagcc     1200

cuaaaacuaa uaacaccccu aaugcuagaa ggcaaaaagu augacgaagc cugcaacgaa     1260

cuaaaucuaa aaguagcaau aaaugaagau aaaaaagauu uucucccagc auucaacgaa     1320

acguacuaca aagacgaagu aacaaaccca guaguccuaa gagccauaaa agaguauaga     1380

aaaguauuaa acgcacuacu caaaaaauau ggaaaggugc auaaaauaaa cauagaacuu     1440

gcccgugaag uaggaaaaaa ucauucucaa agagcaaaaa uugaaaaaga acaaaacgaa     1500

aacuauaagg caaaaaaaga cgccgaacuu gaaugugaaa agcuaggccu uaaaauaaau     1560

ucaaaaaaca uauuaaaacu cagacuauuc aaagaacaaa aagaauuuug cgcauacagu     1620

ggagaaaaaa ucaaaauaag ugaucuccag gacgaaaaaa ugcuggaaau agaccacauc     1680

uaccccuaca gcagaaguuu ugacgacucu uacaugaaua agguucuagu auuuacaaaa     1740

caaaaccaag agaaauuaaa caaaacacca uuugaagcau ucggcaacga cucagcgaaa     1800

uggcaaaaaa uagagguucu agcaaagaac cugccaacaa aaaaacaaaa acguaucuua     1860

gacaaaaauu acaaagauaa agaacaaaaa gacuucaaag acagaaaucu caacgacacc     1920

cgauacauag caagacuagu gcuaaauuac accaaagacu auuuagacuu cuuaccacuc     1980

ucagacgacg aaaacacaaa acuaaaugac acacaaaaag gcucaaaagu ccacguagaa     2040

gcaaaaucag gaaugcuaac uucggcccua aggcauacau ggggauucuc aacuaaagac     2100

agaaacaacc acuugcacca ugccauugac gcaguaauaa ucgcauacgc aaacaacucc     2160

aucguuaaag cuuucagcga uuucaaaaaa gaacaagaau cuaacucagc ggaacuauac     2220

gcaaaaaaaa ucucggaacu agacuauaag aacaaacgaa aauucuucga accauucagc     2280

ggcuucaggc aaaaaguacu cgacaagauc gacgaaaucu ucguuucaaa gccagaacgu     2340

aaaaaaccga gcggagccuu acacgaagaa acauucagaa aagaagaaga auuuuaccaa     2400

ucauacggag gcaaagaagg aguuuugaaa gcacuggaac uugguaaaau acgaaaagua     2460

aacggcaaga uaguaaaaaa cggagauaug uuuagagugg auauauucaa acauaaaaaa     2520

acaaauaaau ucuacgcagu accaaucuau acaauggacu uugccuuaaa aguucuaccu     2580

aauaaagcgg ucgcucgguc aaagaaagga gaaauaaaag acuggauuuu aauggacgaa     2640

aacuacgaau uuuguuuuuc acucuacaaa gacagucuaa uauuaauuca aaccaaagau     2700

augcaagaac cggaauuugu auacuacaac gcuuuuacaa guagcaccgu aucccucauu     2760

guaucaaaac augacaacaa auuugaaacc cuauccaaaa accaaaaaau acuauucaaa     2820

aacgcaaaug aaaaagaagu cauagcaaaa ucaauaggaa uacaaaaccu aaaaguauuu     2880

gaaaaguaua uaguaucugc gcucggugag gucacuaaag cagaauuccg ccaaagagaa     2940

gacuuuaaaa aauga                                                      2955


<210>  4882
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4882
auggcccgca uauuagcauu ugauauaggu auauccucaa ucggaugggc cuucagugaa       60

aacgacgaac uaaaagacug cggaguaaga auuuuuacaa aagcagaaaa cccaaagaca      120

ggcgaaagcc ucgcguuacc cagaagauua gcacgcucag caagaaagag acucgcccga      180

agaaaagcuc gacuaaacca cuuaaaacau cuuauagcua augaauucaa acucaacuau      240

gaagauuacc aauccuucga cgaaucacuc gccaaggcau acaaaggcag uuuaauuagu      300

cccuacgaau uacguuuccg cgcacuaaau gaacuccucu ccaaacaaga uuuugcucgg      360

gugauacuac auaucgccaa acgaagaggg uacgacgaua uaaaaaacuc agacgacaag      420

gaaaaaggag caauacuaaa agcaauaaaa caaaaugaag aaaaauuagc aaauuaucaa      480

ucaguuggag aauaccuaua caaagaauac uuucaaaaau ucaaagaaaa uucuaaagaa      540

uucacaaacg uaagaaacaa aaaagaaucc uaugaaagau gcauagcaca aucauuccua      600

aaagaugagc uuaaauuaau cuuuaaaaaa caacgugaau uuggauuuuc auucucaaaa      660

aaauuugaag aagaagucuu aucaguagca uucuacaaaa gggcucuuaa agauuucuca      720

caccuaguag gaaacugcuc cuuuuucaca gaugaaaaac gagccccuaa aaacagucca      780

cuagcauuua uguuuguagc acuaacuaga auaaucaacc uacucaauaa ccuuaaaaau      840

acagaaggga uucucuauac aaaagaugau uuaaacgccc uauuaaacga gguccuaaaa      900

aacgguacuu uaacauauaa acaaacgaag aaacuucuag gccuaaguga cgacuaugaa      960

uuuaaaggag aaaaagguac auacuuuaua gaauuuaaga aauauaaaga auuuauaaaa     1020

gcacuaggag accauagccu aucccaagau gaccuaaaug aaauagccaa ggauaucacc     1080

cuaauaaaag acgaaaucaa guuaaaaaaa gcccuagcca aauaugaccu aaaucaaaau     1140

caaauagauu cacuaagcaa acucgaauuc aaagaucacc uuaauauauc cuucaaagca     1200

uuaaaauuaa ucacuccuuu aaugcuugag ggaaaaaaau acgaugaagc cuguaacgaa     1260

uuaaauuuaa aaguagcuau caaugaagau aaaaaagauu uucugcccgc auucaacgaa     1320

acuuauuaua aagaugaggu gacaaacccc gugguacucc gagccauaaa agaauaccgc     1380

aaaguccuaa augcacuccu aaaaaaauac ggaaagguac acaaaauuaa cauagaacua     1440

gcaagagaag uaggaaaaaa cuauagucaa agagcaaaaa uagaaaaaga acaaaaugaa     1500

aacuacaaag ccaaaaaaga cgcagaacua gaaugugaaa aauuaggauu aaaaauaaac     1560

ucaaaaaaua uauuaaaacu ucgacuauuu aaagaacaaa aagaguuuug ugccuauuca     1620

ggagaaaaaa uaaaaauauc ugacuuacaa gacgaaaaaa ugcuggaaau agaccacaua     1680

uaccccuaca gucgaaguuu cgaugauuuu uacaugaaua aaguucuagu uuucacaaaa     1740

caaaaccaag aaaaguuaaa ucaaacaccc uucgaagcau uuggaaacga uagugcaaaa     1800

uggcagaaaa uagagguccu agccaaaaac uuacccacua aaaaacaaaa acgaauacua     1860

gacaaaaacu acaaagacaa agagcaaaaa gauuuuaaag acaggaaccu aaacgauaca     1920

agauauauag cucggcuugu gcuaaauuac acaaaagauu accuagacuu cuuaccauua     1980

ucggacgacg aaaauaccaa acuaaacgau acccaaaaag guucaaaagu ucacguugaa     2040

gcaaaaagug gaauguuaac aucugcccuc aggcauacau ggggguuuag cgcaaaagac     2100

cgaaacaacc accuacacca ugcaaucgac gcgguuauaa uagcauaugc aaacaauuca     2160

auugucaaag cauuuuccga cuuuaaaaaa gaacaagaau caaacagugc cgaacuauau     2220

gcaaaaaaaa uuucagaacu agacuauaaa aacaaaagaa aauucuucga acccuuuagc     2280

ggauuuagac aaaaagucuu agauaaaaua gacgaaauau uugucucgaa accagaaaga     2340

aagaagccuu ccggagcgcu acaugaagaa acauuuagga aagaggagga auucuaccaa     2400

ucauauggcg gaaaagaagg cguucuaaaa gcacucgaac uaggaaaaau acgaaaagua     2460

aauggaaaaa uagucaaaaa uggugacaug uuucgcguag acauauucaa acacaaaaag     2520

accaauaaau uuuacgcagu uccaauauac acgauggauu ucgcacugaa aguauuacca     2580

aauaaagcag uagcacgaag uaaaaaaggu gaaauaaaag auuggauauu gauggaugaa     2640

aacuaugaau ucugcuuuuc acucuacaaa gacucacuaa uacuaauaca aacaaaagau     2700

augcaggaac ccgaauucgu cuacuacaau gcauuuacau caucaacugu aucauuaauc     2760

guaucaaaac acgacaauaa auucgaaaca cuaagcaaaa accagaaaau acuauucaag     2820

aaugcgaacg agaaagaagu uauagcaaag ucaauuggga uucaaaacuu aaaaguauuc     2880

gaaaaauaca uaguaagcgc ccucggagaa guaacaaaag cagaauucag acagcgcgaa     2940

gauuuuaaaa aauga                                                      2955


<210>  4883
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4883
auggcaagaa uacuugccuu ugauauaggc auaaguucua uaggcugggc uuucucagaa       60

aacgaugaac uaaaggauug cggagugagg auauucacca aagcugaaaa ucccaaaaca      120

ggagaaucau uagcacuucc aagacgacuu gcuagaagcg caaggaaacg auuagcaaga      180

agaaaagcaa gacucaacca ucugaaacac cuaauugcaa augaauuuaa acucaauuac      240

gaagacuacc aaucuuuuga ugaaagccua gccaaagcau acaaaggauc gcuuauaagc      300

ccauacgaac uacgauucag agcuuuaaau gaauuacuau caaaacaaga cuuugcacgc      360

guaauauuac acaucgcaaa aagacgagga uaugaugaua uuaaaaacuc agacgacaag      420

gaaaaaggag caauccucaa agcuaucaaa caaaacgaag aaaaacuagc caacuaucaa      480

agcgucggug aauacuuaua caaagaauau uuucaaaaau ucaaggagaa uucuaaagaa      540

uucacaaaug uacgaaauaa aaaagaaucc uaugaaagau gcauugcaca aaguuuucuc      600

aaagacgagc ugaaacuaau auuuaagaag caaagagaau ucggauuuuc auucuccaaa      660

aaauuugaag aagaaguacu aagcgucgcc uuuuauaaac gggcucuaaa agacuuuagc      720

caucuugucg gaaacugcag cuuuuucaca gaugaaaaga gagcacccaa aaauucacca      780

cuagccuuca uguuuguugc ucuuacuaga auaauaaacc uacuaaacaa ucuaaaaaac      840

acagaaggaa uauuguacac aaaagaugac cuuaaugccc uucuaaauga auucuuaaaa      900

aauggcacau uaaccuauaa acaaaccaaa aaacuauuag gccucagcga cgauuacgaa      960

uuuaaaggag aaaaaggaac guacuuuaua gaauucaaaa aauacaaaga auucauaaaa     1020

gcgcuaggag aacauaacuu aagucaagac gacuuaaaug aaauagccaa agauauaaca     1080

cugauaaaag augaaaucaa auuaaaaaaa gcucuagcaa aauacgaccu caaccagaac     1140

caaauugacu ccuugucaaa gcucgaauuu aaggaucacc uuaacauuag cuuuaaagca     1200

uuaaaacuaa ucacaccccu uauguuagaa ggcaaaaaau acgaugaagc augcaaugaa     1260

uuaaauuuaa aaauugcaau aaacgaagac aaaaaagacu uccuuccagc cuucaacgag     1320

acguacuaca aagacgaagu cacaaaccca guaguauuac gagcaauaaa ggaauacagg     1380

aaaguacuga augccuuacu aaaaaaguac ggcaaaguuc acaaaauaaa cauagaacuc     1440

gcucgugaag ugggaaaaaa ccacagucaa agagcaaaga uagaaaaaga acaaaacgaa     1500

aacuacaagg caaaaaaaga ugcugaacuu gaaugcgaaa aauuaggacu aaaaaucaau     1560

ucaaaaaaua uucuaaaacu acgcuuauuc aaggaacaaa aagaauucug cgcauauuca     1620

ggagaaaaaa uaaaaauuuc agacuugcaa gaugaaaaaa ugcuagaaau agaccauaua     1680

uaucccuaca gucguuccuu ugacgauucc uauaugaaua aagugcucgu cuucacuaaa     1740

caaaaccaag aaaaacuaaa ccaaacccca uucgaagcau uuggaaauga cagcgccaaa     1800

uggcaaaaaa ucgaaguucu cgcuaaaaac uuaccaacaa aaaagcaaaa gcgaauacua     1860

gacaaaaacu acaaagauaa agaacaaaaa aacuuuaaag auaggaaucu uaacgauaca     1920

agauacauug caagacuugu acucaauuac acaaaagauu aucuagauuu cuuaccauua     1980

agcgacgaug aaaauacaaa acuaaaugac acccagaaag gaucaaaggu ccauguagaa     2040

gccaaaucag gaauguuaac aagugcacua cgacacacau ggggcuucag ugcgaaagac     2100

agaaacaauc acuuacacca ugccaucgac gcaguaauua uagcuuacgc aaacaacucc     2160

auaguuaaag cauuuucaga cuuuaaaaaa gaacaagaga gcaacucagc ugaauuauac     2220

gccaaaaaaa ucagcgaacu agacuauaaa aacaaacgaa aauucuucga accauuuagc     2280

ggauuccgcc aaaaaguuuu agacaaaaua gaugagauau ucguaagcaa acccgaacga     2340

aaaaagccaa guggugcauu acacgaagaa accuuuagga aagaagaaga auucuaucaa     2400

ucauauggug gcaaggaagg cguauuaaaa gcacuagaau uggguaaaau cagaaaagua     2460

aacggaaaaa ucguuaaaaa uggcgacaug uucagaguag auaucuucaa acacaagaaa     2520

acaaacaaau uuuacgcagu accaauauau accauggauu ucgcacuaaa aguucuaccu     2580

aauaaagcag ucgccagauc gaaaaaggga gaaaucaaag acuggauacu uauggacgaa     2640

aacuacgaau ucugcuucag ccuauacaaa gacagucuaa uacuaauaca gacgaaagac     2700

augcaagaac cagaauuugu auacuacaac gcauuuacaa gcucaacagu aucuuuaauc     2760

guuucaaaac acgacaacaa auucgaaacu uuaagcaaaa accagaagau ccuuuuuaaa     2820

aaugcuaaug aaaaggaagu aauugcaaaa ucaauaggca uacaaaaccu aaaaguauuu     2880

gaaaaauaua uagucagcgc cuuaggagaa guaacuaaag cagaguuccg acaacgugaa     2940

gacuuuaaaa aauga                                                      2955


<210>  4884
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4884
augguuagaa ucuuagcauu cgacaucggc aucucaagua uagguugggc auuuagcgaa       60

aacgaugaac uuaaagacug cggaguccga auauuuacaa aaguagaaaa cccuaaaaca      120

ggagaauccu uggcguugcc aagaagacua gcaagaucag cuagaaaaag gcucgcacgc      180

cgcaaagcua gauuaaauca cuugaaacau cuaauagcaa acgaauuuaa acuuaacuac      240

gaagacuauc aaucauucga cgaaucauua gccaaagcau acaaaggauc auuaaucuca      300

ccauacgaau uaagauucag agcacuaaac gaacucuuau caaaacaaga uuucgcacgc      360

gucauacuuc acaucgcaaa aagaagagga uaugacgaca ucaaaaauuc agaugacaaa      420

gaaaaaggag caauccuaaa agccaucaaa caaaaugaag aaaaacuagc caacuaccaa      480

ucaguuggag aauaucuaua caaagaauau uuccaaaaau ucaaagaaaa uuccaaggag      540

uucaccaacg ucagaaauaa gaaagaauca uaugaacgcu guauugccca aucauucuua      600

aaagaugaac uaaaauuaau uuuuaagaaa caaagagaau uuggauucag uuucaguaag      660

aaauucgaag aagaaguacu auccguagca uucuacaaaa gagcacuaaa agacuuuagc      720

cacuuaguag gaaacuguuc auucuucaca gaugagaaaa gagcaccuaa aaacucucca      780

cuagccuuua uguuuguagc ccuaaccaga aucauuaauu uauuaaauaa ccugaaaaau      840

acggaaggua uccuauacac caaagacgau uuaaacgcuc uacugaacga aguacucaaa      900

aacggaacac uaacguacaa acaaacaaag aaacuacuag gacucucaga cgauuaugag      960

uucaaaggag aaaaaggaac auauuuuauc gaguucaaga aauauaaaga guucauaaaa     1020

gcacucggcg aacauaaccu uucacaagac gaccucaacg aaaucgcaaa agauauaaca     1080

cugauaaaag acgaaauaaa acuaaagaaa gcauuagcaa aauacgaccu aaaccaaaau     1140

caaaucgacu ccuuguccaa acuugaauuu aaagaccacc uaaacauuuc auucaaggcc     1200

cugaaacuaa uuacaccccu cauguuggaa gguaagaaau acgaugaagc augcaacgaa     1260

cucaaccuaa aaguagcaau aaaugaagau aagaaagacu uccuuccugc cuucaacgaa     1320

acguacuaca aagacgaagu aacaaaucca guaguacuaa gagcaauaaa agaauacaga     1380

aaaguccuca acgcacuacu aaagaaauau ggaaaaguac acaaaauaaa uauugaacuc     1440

gcaagagaag uagguaaaaa ccauucgcaa agagccaaaa uagaaaaaga acaaaacgaa     1500

aacuacaaag cuaagaaaga cgcagaacua gaaugcgaaa aacucggauu aaaaauaaau     1560

ucuaaaaaca uccuaaaacu acgacuauuc aaagaacaga aagaguucug cgcuuauuca     1620

ggagagaaaa uuaaaaucuc agaucuacaa gaugagaaaa ugcuagaaau agaucacaua     1680

uacccuuacu cacgcucauu cgacgacucu uacaugaaca aagugcucgu cuucacaaaa     1740

caaaaccaag aaaaacucaa caaaacucca uuugaggcuu ucggaaauga cucagcaaaa     1800

uggcagaaaa uagaaguacu agcuaaaaac uuaccaacua agaaacaaaa acgaauccua     1860

gauaaaaacu auaaagacaa agaacaaaaa gacuucaaag acagaaaucu aaaugacacg     1920

agauacauag cacgucuggu acugaauuac acaaaagauu aucuggacuu ccuaccacuc     1980

agcgaugacg aaaacacaaa auugaacgau acacaaaaag gaucuaaagu acauguagaa     2040

gcaaaaucag gaaugcuaac cucagcacua agacacacau ggggauucuc aacuaaagac     2100

agaaacaauc aucuacacca cgcaauagac gcagugauua ucgcauaugc aaauaauuca     2160

auagugaaag ccuucagcga cuucaagaaa gaacaagaaa guaacucugc agaacucuac     2220

gcuaagaaaa uaucagaacu cgauuauaaa aacaaaagga aauucuucga accauucucc     2280

ggguucagac aaaaaguacu agacaaaauu gacgaaauau ucguaagcaa accagaaaga     2340

aagaaaccaa gcggagcacu ucaugaagaa acauuuagaa aagaagaaga guucuaucaa     2400

ucauacggag gaaaagaagg aguacuaaaa gcauuagaac ucggaaaaau acgaaaagua     2460

aacggaaaaa uagucaaaaa cggcgauaug uuuagaguag acauauucaa acacaagaaa     2520

accaacaaau ucuacgcagu acccaucuau accauggacu ucgcguugaa aguauuaccc     2580

aauaaagcag uagcaaggag caagaaagga gaaauaaaag auuggauacu aauggaugaa     2640

aauuaugaau uuugcuucuc auuauacaaa gauucuuuaa uauuaauaca aacaaaagac     2700

augcaggaac cagaguucgu auauuauaau gcauucacau cuucaacugu aagccuaauc     2760

gucuccaaac acgauaacaa auucgaaacu cucagcaaaa aucagaaaau acuguucaaa     2820

aacgcaaacg aaaaagaagu aauagcuaaa ucaauaggaa uacaaaaucu aaaaguauuc     2880

gaaaaauaca uuguaucagc uuuaggagaa guaaccaaag cugaauuuag acaaagagaa     2940

gacuucaaga aauga                                                      2955


<210>  4885
<211>  2955
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4885
auggccagga uauuagcuuu cgauauaggc auaagcagca ucggaugggc guuuagugag       60

aaugaugagc ucaaagacug uggcgugcgc auauucacaa aagcagaaaa cccuaaaacc      120

ggcgaaagcu uagcccuacc aagacggcuu gcuagaagcg ccagaaaaag auuagcuaga      180

cgaaaagcaa gacuaaacca ucuaaaacac uuaauagcca acgaauuuaa auuaaauuac      240

gaagauuacc aaucauucga ugaaucgcua gcaaaagcgu auaaaggauc guuaauaagc      300

ccauaugaac uucgauuccg agcacuuaau gaauuauuau caaaacaaga cuuugccaga      360

guaauauugc auauagcaaa aagaagagga uaugaugaca uaaaaaauag cgaugacaaa      420

gaaaaaggag cuauccuaaa agcaauaaaa cagaaugaag aaaagcuagc aaacuaccaa      480

uccguaggag aauaucuuua caaagaguac uuccaaaaau uuaaagaaaa uucuaaagaa      540

uucacaaacg ugagaaauaa aaaagaauca uaugaacgau guauagcuca aucauucuua      600

aaagaugaac uaaaacuuau cuucaaaaaa caacgagaau uuggauuuuc guucucaaaa      660

aaauucgaag aagaaguuuu aucagucgcc uucuacaaac gugcuuuaaa ggauuuuuca      720

caucuuguag gcaacuguuc uuucuucaca gacgaaaagc gcgccccaaa gaauagucca      780

cuugcguuca uguucgucgc gcucacaaga aucauaaacc uccuuaauaa cuugaaaaac      840

acagaaggca uuuuauacac gaaagaugac uuaaacgcac uucuaaacga aguccuaaaa      900

aacgggacuc ucacuuacaa acaaaccaaa aaacuccuag gacugucuga cgauuacgaa      960

uucaaaggug aaaaaggaac cuacuuuaua gaauucaaaa aauacaaaga auuuauaaaa     1020

gcccuaggcg aucacuccuu aucgcaagac gaucuaaaug aaauagccaa agauauuacc     1080

cuaauaaagg augaaauuaa acuaaaaaaa gcauuagcaa aguaugaucu uaaucaaaac     1140

caaauugacu cacuaucaaa acuagaauuu aaggaucacc uaaauauauc guucaaagca     1200

uuaaaacuua ucaccccauu aauguuggaa ggaaaaaaau acgacgaagc gugcaacgaa     1260

uuaaauuuaa aaguagcaau aaacgaggau aaaaaggacu uccuacccgc auucaacgaa     1320

acauacuaca aagaugaagu gacaaauccu guaguauuac gagcuauaaa agaauauaga     1380

aaaguccuua acgcgcuacu aaaaaaauau ggcaaaguac auaaaauaaa caucgaauua     1440

gcaagggaag uuggcaaaaa cuauucucaa agggcaaaaa uugaaaaaga acaaaacgaa     1500

aacuacaagg caaaaaagga cgcagaacuu gaaugcgaaa aacucggccu aaaaauuaau     1560

ucuaaaaaca uccuuaaacu aagacuuuuc aaagaacaaa aagaauuuug ugcauacuca     1620

ggugagaaaa ucaaaauauc agaccuacaa gaugaaaaaa uguuagaaau agaucauauu     1680

uacccguauu caagaucauu cgacgauuca uacaugaaua aaguauuagu cuucacaaaa     1740

caaaaccaag aaaaacuaaa ucaaacucca uucgaagcau uuggaaauga cagcgcaaaa     1800

uggcagaaaa uagaaguauu agcuaaaaau uuaccaacaa aaaaacaaaa aagaauuuua     1860

gauaaaaauu auaaagauaa ggaacaaaaa gacuuuaaag acagaaaccu aaacgacacc     1920

cgguacauag cacgauuagu acucaauuac acuaaagacu accuagacuu ucucccacua     1980

ucagacgaug aaaauacaaa acuaaaugac acucaaaaag gaucaaaagu ccacguagaa     2040

gcaaaaucag gaaugcucac aucagcacug cgucauaccu ggggcuucuc ggcaaaagac     2100

cgaaacaacc aucuacacca cgcaauugau gcaguaauaa ucgcauaugc aaauaacucc     2160

aucgucaaag cauucagcga cuucaaaaaa gaacaggaau ccaauagcgc agaacuauau     2220

gccaaaaaaa uauccgaauu agacuacaaa aacaaacgaa aauucuucga acccuucuca     2280

ggauuuagac aaaagguacu agauaaaaua gaugaaauau ucguaaguaa accagagcgc     2340

aaaaaaccaa gcggugcacu acacgaagaa accuuucgaa aagaggaaga guucuaucaa     2400

agcuauggag gaaaagaagg cguacuuaaa gcguuagaac uagguaaaau acgaaaggua     2460

aacggaaaaa uaguuaaaaa cggcgacaug uuucgaguag acauauuuaa acauaaaaaa     2520

acaaacaaau ucuaugcagu accaaucuac acaauggauu uugcguuaaa aguauuacca     2580

aacaaagcgg uagcacgauc caaaaaaggc gagaucaaag acuggauauu aauggacgaa     2640

aauuacgaau ucuguuucag cuuauauaaa gacuccuuaa uuuuaauaca gaccaaagac     2700

augcaagaac cagaauuugu auauuauaac gcuuuuacau cauccacugu uucauuaaua     2760

guaucuaaac augacaauaa auuugaaacc cuaucaaaaa aucaaaaaau uuuauucaaa     2820

aacgcaaaug aaaaagaagu aaucgcaaaa ucgaucggua uccagaacuu aaaaguauuc     2880

gaaaaauaca ucguauccgc ccuaggugaa guuacaaaag ccgaauucag acaaagggaa     2940

gacuucaaaa aauga                                                      2955


<210>  4886
<211>  2958
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4886
auggcaaaaa uacuagcguu ugacauugga auaucgucaa uaggaugggc cuucguagaa       60

aaugaugagc ucaaagacug cggaguccga aucuuuacua aggcagaaaa ccccaaaaca      120

ggggaaaguu uagcacuacc ccgaagacuu gcccgcucag cuagaaagag acuagcgcga      180

agaaaagcua gacucaauca ccucaaacac cugauagcaa augaauucaa acuuaacuac      240

gaagacuacc aaucuuuuga cgaaucacuc gcaaaagcau auaaagggac acuaauaagu      300

ccauaugaac uacgauucaa agcauuaaac gaauuauuau caaaacaaga ccuagcaaga      360

guaaucuuac auauagcaaa aagaagagga uacgauaaca uaaaaaacuc cgacgacaaa      420

gaaaaaggag caauucucaa agcaguaaaa gaaaacgaag aaaagcuagc caauuauaaa      480

uccguaggag aauaucuaua caaagaguau uuccaaaaau uuaaagaaaa uagcaaagaa      540

uucauaaacg uucguaacaa aaaagaaagc uacgaaagau gcauagcaca auccuuccua      600

aaagacgaau uaaaauuaau auuccaaaaa caaagagaau ucggcuuuaa cuucaguaaa      660

aaauuugaag aagaaguauu aagcguagca uucuauaaac gagcauuaaa agacuucuca      720

caccuuguag guaauuguuc uuuuuucgua gacgaaaaac gcgcgccaaa aaauucacca      780

cuagccuucg aauuuguugc acuaacacga auaguaaauc uacucaauaa ccucaacaaa      840

aauaauucau acauaaaaga cgacuuaaac gcacuucuau cagagguccu caaaaacggc      900

acacuaacau acaagcaaac uaaaaaaauc cuaggauuau cagacgacua cgaauuuaaa      960

ggccaaaaag gcaauuacuu uaucgaauuu aaaaaauaca aagaauucau aaaagccuua     1020

ugcgaacaua auuuaaauca agacgaucua aaugaaauag cuaaagacau aacucugguc     1080

aaagacgaaa caaaauuaaa aaaagccuug gcgaaauacg acuuaaacca aaaccaaaua     1140

gaaaaucuuu cuaaaauaga auucaaagac cauuuaaaua uaaguuucaa agcacuaaaa     1200

uuaaucguac cacugaugcg agaaggaaaa aaauaugaug aaguuugcaa cgaacucaac     1260

cucaaaacag caaucaauga agauaaaaaa gaguuuuuac cagcuuuuaa ugaaacauau     1320

uacaaagacg aagugaauaa cccggucgua cuacgagcua ucaaggaaua cagaaaaguc     1380

cuaaacuccu uacugaaaaa auacggcaaa guacacaaaa uaaauauaga gcuagccaga     1440

gaaguuggaa aaaaucacag agaacguuca aaaauagaaa aagaacaaaa ugaaaauuuu     1500

aaagcaaaaa aagacgcaga acuggaaugc gaaaaauuag gucuaaaaau aaacaguaaa     1560

aauaucuuaa aauuacgcuu auucaaagaa caaaaagaau ucugcgucua cagcggagaa     1620

aaaauaaaaa ucucagaccu ucaagacgaa aaaaugcuag aaauagacca caucuacccu     1680

uauagcagau cauucgacga uagcuacaug aacaaaguac uagucuuuac uaaacagaau     1740

caagagaaac uaaacaaaac accauucgag gcauucggaa aagacgaugu aaaguggcaa     1800

aaaaucgaaa cucuagccaa aaacuugcca gcgaaaaaac aaaagcgaau ccuagacaaa     1860

aacuacaaag acaaggaaca aaaagauuuc aaagacagga accucaauga cacccgauau     1920

auagcacgac uaguacugaa cuacacuaaa gauuaucuag aauuucuacc auuaucagau     1980

gacgaaaaua uaaaacucaa cgacacacaa aaaggaucca aaguacaugu cgaagcaaaa     2040

ucgggaaugu uaacaucagu acuaagacac acauggggau ucucgacaaa ggacagaaac     2100

aaucaccuac accaugccau agacgcagua auaauagccu acgcaaaugc aaguguagua     2160

aaagccuucu cagauuucaa gaaagaacaa gaaaauuaca cagcagaauu auacgcaaaa     2220

aaaaucaacg aacucgacuu caaaaauaaa aggaaauuuu uugagcccuu cucaaacuuc     2280

agacaaaaag uacuagaaaa aauagaugaa auauucguaa guaaaccuga aagaaaaaaa     2340

cccuccggcg cauuacauga agaaacauuc uacaaagaag aagaauucua caaagaguau     2400

ggaggaaaag aagggguauu aaaagcacua gagcuaggaa aaauacgaaa agucaacggc     2460

aaaaucguua guaacgcuaa cauguuuaga guagauauuu ucaaauauaa aaagacgaac     2520

aaauucuaug caguacccau uuacacaaug gacuuugcac ucggcauuuu accaaacaaa     2580

gcaguagucg gagguaaaga caaacaagga auaauaaaag auuggcuuuu aauggacgaa     2640

aauuaugaau uuugcuuuuc acuauacaaa gauagccuaa ucuuguuaca gaccaaggac     2700

augcaagagc cagaacuagu cuacuucaaa ucauucaaaa guucuacagc aggacuagua     2760

guaucaaaac acgacaacaa auucgagaau uuaaaugaaa accaaaaaaa gcuauuuaag     2820

ucagcuaacg aaaaaaaagu cgucgcagag ggaauaggaa uacaaaacuu aaaaguuuuc     2880

gagaaauaca uaguauccgc acucggugaa auaacaaagg cagaaauaaa agaaagagaa     2940

aacuucaaaa aaaaauga                                                   2958


<210>  4887
<211>  2961
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4887
augccaagcg auccagcaga agccaaacca uuagaauuau uggauccaua ugccuugagg       60

gcacaaggac uagaccaagc auugccccuc acacacuuug guagagcccu cuuccaucua      120

aaucaacgaa gagguuucaa aaguaaucga aagacagauc gaggcgauaa cgaaaaugga      180

aaaaucaaag augcgucaca acguuuagac ugggcaaugc augaagcaaa agcaagaaca      240

uauggagagu uuuuacaucu aaggagacaa aaagcaccag acccuagaca aguuccaaca      300

guacguacaa ggcucucagu cgguaaaaga ggaggaccag augcaaaaga agaagcugga      360

uaugacuuuu acccagauag aaggcauuug gaagaagaau uucaugaacu augguccgca      420

caagcacaau uucaucccga acucacacag gaccuacgcg acuugguauu cgaaaagaua      480

uuuuaucagc guccucuuaa agaaccuaaa gucgguuuau guuuauucac uucagaagaa      540

agauuagcga aagcccaucc auuaacacaa cgacgaguuu uguacgaaac aguaaaucaa      600

uuaaggauaa cagcugaugg aagaagcguu aggccacuaa guagggaaca aagagaucaa      660

auuaucuugg cacuagacac aaagaaaccu acaaaaucac uuaacucaau gguaguaaca      720

cugcaagccc uuggaaaagu acuuaaauua cgagaaggag aaagguucac ucuagaauca      780

ggcguaagag aggcuauagc augcgaccca gugcgggcag caaugcuugc aacauucggu      840

ccaagauggu cagcacuuga uuggccagaa caauggcaag uuauaucaag agugagaaaa      900

cuacaagaug acaaagauuu ugcugagcua gucgaauggu uaauagaauu guacggagua      960

gacaaagaag cagccguaaa aaucgcaaau acccacuuac cagaaggaua uggaagacua     1020

ggacugacgg caacgacaag gauauuggau cggcuaaaau caaauguauu uacauacgca     1080

caagcaguag cugcaugugg auggcaucau uccgaccaga gaacaggaga augccuagau     1140

agacuaccgu acuauggagc aguuuuagau agacacguca uaccaggcac auaugacgaa     1200

aaugaugaug acaucacaag auuuggaaga auaacaaauc caacuguuca uaucggacua     1260

aaucaacucc gaaggcuagu uaauaaaauu auagaaguuu acggcaaacc aaaccaaaua     1320

guaguagaac uugcacgaga auuaaaacaa agugaacaac agaaaaacga cgcaauaaaa     1380

uuaauaagag acacaacaac agcagcaaaa caaagaucag aaaaauuaga agaauuggga     1440

auaacagaca acgguagaaa uagaauguua cuaagacuau gggaagaccu aaacccagac     1500

gaugcaauga gaagauucug cccauacacc ggacaaagga uauccgcagc aaugaucuuc     1560

gauggaucuu gcgauguaga ucauauacua ccuuauucaa ggacucuaga ugaugguuuc     1620

gcaaauagaa cacuaugucu aagggaggca aaccgagaaa aacguaacca aacuccaugg     1680

gaagcauggg gaggaacucc gaauugggaa gcaauagaaa caaaucuaaa gaauuuaccu     1740

gaaaauaaaa gauggagauu ugcgccagau gcaauggcaa gguuugaagg agaaaacgac     1800

uuccucgaua gagcucuggu agacacgcag uaccuagcaa gaauaaguag aacauaucua     1860

gauacccuau ucacagaagg uggccacgug uggguaguac caggacgcuu gacagaaaug     1920

cuuagaagac acuggggccu uaacucuuug cuaacagaua aagauagagg ugccguaaaa     1980

agcaaaaacc gaacagacca uaggcaccau aguauagaug cagcaguagu agcugcgacu     2040

gacagaaguu uacuuaauag aauaucgcga gcagccgguc aaggagaagu agcaggacag     2100

agugcagaau uaauugcaag ggagacucca ccaccuuggg aagguuuccg aaaugaucua     2160

aaaagucaac uaggaagaau uauuguaagu cacagagcag aucauggacg gauagacaga     2220

caagcaagaa aacaaggacg ggacucuaca gccggucaac uacaucaaga aacagcguac     2280

uccauaguug augauacaag aguagcaagc agaaucgacu uuuuaagugu aaaaccagca     2340

caguuacuug accaaccagg uagaucagga cagguaaggg aucuacaauu aagaaaagcg     2400

uuacgaguag cuacaggcga ccugaccgga aaagacuacg agaaagcacu aagggauuuc     2460

gcugcaaaac cagguccaua uaaagguaua aggagaguac gaauaauaaa acccuugcaa     2520

gaacaagcgc gagugcccgu cccaguucuu gaccccauaa aagcauacca gggaggauca     2580

aaccauguau ucgaaauaug gcauuuacca gacggcaagg uagaaccaca agugauaacc     2640

ucauuugaag cacaugcguu agaaguggaa aagcguccac acccugcagc uaaaagacua     2700

uuaagaguac auaaaggcga uaugguagca cuaaaacgug aaggaagaac aguaguagga     2760

cacguucaac agaugcauau uaaaaauggu uuauucauag uuccgcauaa ugaagcuaau     2820

gccgaugcca gaaauucuga caggacagac ccuuuuaaau ggauucaaau ugcugcucag     2880

ccagcaauag cagcagguau acgucgugug gcaguggacg aaauagggag guuaagagau     2940

ggcggagcaa aaacaauaug a                                               2961


<210>  4888
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4888
auggcaagaa uucuagcauu ugacauagga aucucaucca uaggcugggc guucucugaa       60

aaugacgagc ucaaagauug ugguguuaga auauucacaa aagcagaaaa ccccaaaacc      120

ggcgaaucau uagcacuacc cagacgacua ucuagaagcg cuagaaagcg ccuggcacga      180

agaaaagcaa gacuuaacca uuuaaaacau cugauugcaa augaauucaa auuaaauuau      240

gaagacuauc aaagcuuuga cgaaagccua gccaaggcgu auaaaggguc acuuauauca      300

ccauacgaac uaagauucag ggcucuaaac gaauucuuau caaaacaaga cuucgcaaga      360

guaauacucc auauagcaaa acguaggggg uaugacgaca uaaaaaauaa cggagaugag      420

gaaaagggcg aaauacuuaa agcaaucaaa caaaaugaag aaaaguuagu cucauaccaa      480

ucaguaggug aguaccuaua caaagaguau uuucaaaaau ucaaagaaaa cuccaaagaa      540

uucauaaaug uccgaaauaa aaaagaauca uacgaacgau guaucgcaca aucauuucuu      600

aaagacgaac uuaaauuaau cuuccaaaaa caacgagaau ucggauucag cuuuucuaaa      660

aaauuugagg aggaaguauu aucaguagcc uuuuacaaac gcgcauuaaa agacuuuagc      720

cacuugguag gaaauugcuc auucuuuacu gacgaaaaac gagcgccaaa aaauucgcca      780

uuagcauuca uguucguugc auuaacccga auaauaaacu uacuaaacaa ucuaaaaaau      840

acggaaggaa uacuauauac caaagacgac cugaauacgc uacuaaacga aguauuaaaa      900

aacggaacuc uaaccuacaa acaaacuaaa aaauuacuag gauuauccaa cgacuaugaa      960

uucaaaggag aaaaagguac auauuuuauc gaauuccaga aauauaaaga auuuguaaaa     1020

gccuuaggag aacacaaucu gagucaagac gaucuaaaug aaauugcaaa agacaucacc     1080

cuaauaaaag acgaaauaaa auuaaagaaa gcucuagcca aauacgaccu aaaccaaaau     1140

caaauagauu cccugagcaa acuagaauuu aaagaccauc uaaauauaag cuuuaaggcc     1200

cuuaaacuaa uaacuccucu aaugcuagaa gguaaaaagu augacgaagc guguaacgaa     1260

cuuaaccuaa aaguagcuau uaaugaagau aaaaaagauu uuuuaccggc auucaaugaa     1320

acauauuaca aagaugaagu aacuaaccca guaguacuca gagcaaucaa agaauauaga     1380

aaaguacuca augcucugcu caaaaaauac ggaaaaguuc acaaaaucaa cauagaacua     1440

gcaagagaag ucggaaaaaa ccauucccaa agagcaaaaa uugaaaaaga acaaaaugaa     1500

aacuauaaag cuaaaaaaga ugcagaacuu gaaugugaaa aauuaggacu caaaaucaac     1560

ucaaaaaaca uacuaaaacu acgacuauuc aaagaacaaa aagaauuuug cguuuauagu     1620

ggagaaaaaa uuaaaauauc cgaccuacaa gaugaaaaga ugcucgagau agaccauaua     1680

uauccauaca guagaucauu cgacgauucc uauaugaaua agguucuagu cuucacaaaa     1740

caaaaccaag aaaaauuaaa uaaaacacca uucgaaaccu ucgguaauga ucgcgucaaa     1800

uggcaaaaaa uugagguacu cgccaaaaau cuaccaacga aaaaacaaaa gcgcauauua     1860

gauaaaaacu acaaagacaa agaacagaaa gacuucaaag acaggaaccu aaaugacaca     1920

agguacauag caaggcuugu acuaaacuac acaaaagacu accuagacuu ucuaccacua     1980

ucagacgacg aaaauaccaa auuaaacgau acccaaaaag guaguaaagu acacguagaa     2040

gcaaaaucgg gaaugcuaac aaguacguua cgacacacuu ggggauucag uaccaaagau     2100

cgagauaacc auuuacacca cgcaauugac gcagcaauaa uagccuaugc uaacaauagu     2160

auaguaaagg ccuuuuccga cuuuaaaaaa gaacaagagu caaacacagc ugaacuauac     2220

gccaaaaaaa uuucugagcu agacuacaaa aacaaaagaa aauucuuuga accauucagc     2280

ggcuuuagac aaaaacuauu agauaaaaua gaugaaaucu uuguaucaaa accagagcgg     2340

aaaaaaccaa gcggggcucu ucaugaagaa acguucagaa aagaagagga auucuaccaa     2400

uccuacggcg gcaaagaagg aguucuaaaa gcacuagaac uaggaaaaau cagaaaagua     2460

aacgguaaaa uggugaaaaa uggagauaug uuccguguug acaucuuuaa acauaaaaaa     2520

acuaacaaau ucuaugcagu accaauuuau acaauggauu uugcacucaa aguacuacca     2580

aauaaagcag uaguccaagg aaaagacaaa aaaagcggac uaauuaaaga cuggauucug     2640

auggaugaaa acuaugaauu uuguuucuca cuauauaaag acucacuaau ccuaauacaa     2700

acaaaggaca ugcaagagcc agaacuugua uauuaugaaa gacuagacuc uagcaacgcc     2760

ucauuaacgg uuucaaaaca ugauaauaaa uuugaaacau uaagcaaaaa ccaaaaaaua     2820

uuauucaaaa acgcaaacga gaaagaaguc auagcgaaau ccauaggaau ccaaaacuua     2880

aaaguauuug aaaaauauau cgugagcgcc cucggcgaag ucacuaaagc agaauucaga     2940

caacgagaag acuuuaaaaa auga                                            2964


<210>  4889
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4889
auggcacgaa uucucgcauu cgacauugga auaagcucca uaggaugggc auucucagaa       60

aacgacgagc ucaaagauug uggcguaaga aucuucacaa aagcagagaa ccccaaaaca      120

ggagaaucau uagcauuacc aaggagacuc gccagaagcg cccgaaaacg ucucgcaaga      180

aggaaggcca gauuaaacca ccuaaaacau cuuauagcaa augaauucaa acuaaauuac      240

gaagacuauc aaaguuucga cgaaucacug gcgaaagcau acaaagggag ccucaucuca      300

ccauacgaac ucagauucag agcauuaaau gaacuacucu ccaaacaaga cuucgcaaga      360

guuauacuac acauagcaaa aagacgagga uaugacgaca uaaaaaacuc agacgauaaa      420

gaaaagggag ccauauuaaa agccauaaaa caaaaugaag aaaaauuagc aaacuaccaa      480

ucaguaggag aguaucuaua caaagaauac uuccaaaaau ucaaagaaaa cuccaaagaa      540

uucacaaacg uaagaaacaa aaaagaaucc uacgaacguu guaucgccca aaguuuuuua      600

aaagacgaau uaaaacucau uuucaaaaaa caaagagaau ucggcuuuag uuuuagcaaa      660

aaauuugaag aagaagucuu aaguguagca uucuauaaaa gagcauuaaa agacuucucu      720

caucucgugg gaaacuguuc cuucuucacu gacgaaaaaa gagccccaaa aaauucaccu      780

uuagcauuua uguuuguagc ccuaacaaga auaauuaacu uacuaaacaa ccuaaaaaac      840

acagaaggaa uacuauacac aaaagaugac uuaaacgcau ugcuuaacga aguauuaaaa      900

aacggaacau uaacauacaa gcaaacaaaa aaacuucuag gacuauccga cgauuaugaa      960

uucaaaggcg aaaaaggaac cuacuuuauc gaauuuaaaa aauacaaaga auucauaaag     1020

gcacuaggag accacucccu gucucaagac gaucucaacg aaauagcuaa agacaucacu     1080

cuuauaaaag augaaauuaa acucaaaaaa gcauuagcca aauacgauuu gaaucaaaau     1140

caaauagaua gucucaguaa acuagaauuu aaagaucacc uaaacauaag uuucaaagca     1200

uuaaaacuua uaacgcccuu aaugcucgaa ggaaaaaaau acgaugaagc uugcaaugaa     1260

cuuaaucuaa aagucgcaau uaacgaagau aaaaaagauu uucucccagc guucaaugaa     1320

accuacuaca aagacgaagu aacgaaucca guaguucuca gagcgauaaa agaauaucga     1380

aaaguucuca augccuuacu aaaaaaauac ggaaaagucc acaaaauuaa caucgaacua     1440

gcaagagaag uagggaaaaa uuauucacaa agagcaaaaa uugaaaaaga acaaaaugaa     1500

aauuacaaag cuaaaaaaga cgcugaacuc gaaugcgaaa aacuuggcuu aaaaauuaac     1560

ucgaaaaaca uacucaaacu acgacuauuc aaagagcaaa aagaauuuug cgcauauucg     1620

ggugaaaaaa ucaagaucuc ugauuuacaa gaugaaaaga ugcuugaaau agaccacaua     1680

uauccauaua gcagaucauu cgaugacagc uauaugaaca aaguucucgu uuucacgaaa     1740

caaaaucagg aaaaacuaaa ucaaacaccu uuugaagcau ucggaaacga cucugcuaaa     1800

uggcaaaaaa uagaaguacu ugcaaaaaac cuuccaacca aaaaacaaaa aagaauccuu     1860

gacaagaauu acaaagacaa ggaacaaaaa gacuucaagg aucgaaacuu aaacgacacu     1920

cgcuauaucg cucgucucgu cuuaaacuau acaaaggacu acuuagauuu ucucccacuc     1980

ucagaugacg aaaacacaaa acuaaaugac acacaaaaag gaucuaaagu acauguagaa     2040

gcaaaaucag gcaugcucac uucagcauua aggcauacau ggggauucuc agcuaaggac     2100

cggaauaacc accuacacca ugcaauagau gcaguaauca ucgccuacgc caacaacagc     2160

auaguaaaag cuuucaguga cuucaaaaaa gaacaagaau cuaacagugc cgaacucuau     2220

gcaaaaaaaa uuagcgaacu ggacuacaaa aauaaaagaa aauucuucga accauucuca     2280

ggauuccggc aaaaaguacu agacaaaauc gacgaaauau ucguaucaaa acccgaaaga     2340

aaaaaaccca guggcgcccu acacgaagaa accuucagaa aagaagaaga auuuuaucaa     2400

ucauacggag gaaaagaagg agugcuaaaa gcguuagaac uaggaaaaau cagaaaagua     2460

aacgguaaaa uaguaaaaaa uggcgacaug uuccgagucg auaucuuuaa gcacaaaaaa     2520

accaacaaau ucuaugcagu gccaauauau acaauggauu uugcacuaaa aguuuuaccu     2580

aacaaagcug uggcacgauc aaaaaaagga gaaaucaaag auuggauauu aauggaugaa     2640

aauuacgaau ucugcuuuuc guuauauaaa gacucacuaa uacuuaucca aacuaaggau     2700

augcaagaac cagaauuagu auacuuuaac gcauucacuu caucaacagu aucauuaaua     2760

guaaguaaac acgauaacaa auuugagaca cuaagcaaaa accaaaaaau acucuuuaaa     2820

aaugccaaug agaaagaagu gauagccaaa ucaauaggaa uacaaaaccu uaaaguuuuc     2880

gaaaaauaca ucguaagcgc acuaggagaa gucacaaaag cagaauuccg ccaaagagaa     2940

gauuucaaga augaauuacg auga                                            2964


<210>  4890
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4890
auggcaagga uccuggcauu cgacauagga auaagcucaa ucggaugggc auucagcgaa       60

aaugaugaau uaaaagacug cggcguacgc aucuuuacaa aagccgaaaa cccaaaaaca      120

ggagagagcu uagcccuccc acgccgacuu gcaagaagcg cacguaaacg auuagcuaga      180

agaaaagcaa gauuaaacca uuuaaaacau uuaauagcua augaauuuaa acuaaauuac      240

gaagauuacc aaucuuuuga cgaaucuuua gcaaaagcau acaaaggcuc acucauaagu      300

ccuuacgaac ucagauucag agcccuaaac gaacuauuaa guaaacaaga uuucgcgcgg      360

guaauccugc auauagccaa aagaagagga uaugacgaca uuaaaaauaa uggcgacgaa      420

gaaaaaucag aaauacucaa agccauaaaa caaaaugaag aaaaacucgu gaacuaucaa      480

agcguuggag aauauuuaua caaagaauau uuucaaaaau ucaaagaaaa cagcaaagaa      540

uuuauaaaug uaaggaacaa gaaagaaagc uacgaacggu guauagcaca aaguuuucua      600

aaagaugaac uaaaauuaau auuucaaaaa caaagagagu ucggauucuc auucucgaag      660

aaauucgaag aagaaguauu aagcguagca uuuuacaaac gcgcacuaaa agacuuuucu      720

caucuaguag gcaacugcuc auuuuucacg gacgaaaaac gagcacccaa aaacucccca      780

uugguauuca uguuuguagc cuuaacgaga auaauaaacc ugcuuaauaa ucucaaaaau      840

acagaaggaa uauuauacac aaaagacgac uugaacgcau uacucaauga aguacuaaag      900

aaugguacac uaacauacaa acaaacaaag aaauuauugg gccucaguga cgauuaugag      960

uucaaaggug aaaaaggcac auacuucaua gaguucaaga aauauaaaga guucaucaaa     1020

gcgcucgacg aacacagcuu aucacaagac gauuuaaacg aaauagcaaa agacauuacc     1080

cuaauaaaag acgaaauuaa acuaaagaaa gcacucgcca aauacgaccu aaaagaaaau     1140

caaauugauu cauuaucaaa acuagaauuu aaagaccacc ucaacaucag cuucaaagcu     1200

cuaaaauuaa uaacaccauu aauguuagaa ggcaaaaagu acgaugaagc augcaacgaa     1260

cuaaaucuca agguugccau aaacgaagac aagaaggauu uccuaccagc auucaacgaa     1320

acauauuaca aagacgaagu aaccaaucca guaguacucc gcgcuauaaa agaauauaga     1380

aaaauauuaa acgcucuauu aaagaaauac ggaaaaguuc auaaaauaaa uauugaacua     1440

gcacgcgaag uagguaaaaa ccacucgcaa cgagcuaaaa ucgaaaagga acaaaacgaa     1500

aauuauaaag caaagaaaga ugcagaacuc gaaugcgaaa aauugggcuu aaaaauaaau     1560

ucuaaaaaca uccuaaaacu aagacuauuu aaagaacaaa aagaauuuug cguauacuca     1620

ggcgaaaaga ucaaauuauc agacuugcaa gaugagaaaa uguuagaaau ugaucauauc     1680

uacccuuaua gcaggagcuu cgacgacuca uacaugaaua aaguauuagu auucgccaaa     1740

caaaaccaag aaaaacuaaa uaaaacccca uucgaagcau ucggaaauga cucaacgaaa     1800

uggcagaaaa uagaaguccu aacuaaaaac cuaccaacaa agaaacagaa aagaauccua     1860

gacaaaaacu acaaagacaa agaacaaaaa gacuucaaag acagaaaucu aaaugauaca     1920

cgauacauag cacgacucgu ccuaaauuac accaaagauu acuuagauuu ccuaccacua     1980

ucagacgacg aaaacacaaa acucaacgau acacaaaaag gcaguaaagu acauguggaa     2040

gcaaaaucag gaauguuaac cagugcauua agacacacgu ggggauucag uacuaaagac     2100

cgaaauaacc aucuccacca cgccauagac gcaguaauaa uagcuuaugc gaacaacagu     2160

auaguaaaag cauuuuccga cuuuaagaaa gaacaagaaa gcaacagcgc agaacuauac     2220

gccaagaaaa uaagugaauu ggacuauaaa aacaaaagaa aauuuuucga accauuuagc     2280

ggauucagac aaaaaguacu agacaaaaua gacgaaauuu ucgucucuaa accagaacga     2340

aagaaaccau cgggcgcacu acacgaagaa acuuucagaa aagaagaaga guucuaccaa     2400

uccuacggcg gaaaagaagg gguacucaaa gcauuagaau uaggcaaaau aagaaaagua     2460

aacggaaaaa uagugaaaaa cggcgacaug uuucguguag acauauucaa acauaagaaa     2520

accaacaaau ucuacgcagu acccauauac acaauggauu uugcacuaaa aguacuaccc     2580

aacaaagcag uagcgcgauc uaagaaagga gaaauaaaag acuggauucu gauggacgaa     2640

aacuaugagu ucugcuucuc cuuauacaaa gauucucuaa uccuaauaca aacaaaagau     2700

augcaagaac ccgaacucgu auauuucaau gcuuuuacaa guagcacagu uagcuuaaua     2760

guaagcaaac acgacaacaa auucgaaaca uuaaguaaaa aucaaaagau acuauuuaaa     2820

aaugccaacg aaaaagaagu aauagcgaaa ucaauaggaa uacaaaaccu aaaagucuuc     2880

gaaaaauaca uuguaucagc acuuggagaa guaacaaaag cagaauuucg acaacgcgaa     2940

gacuuuaaaa acgaacuccg auga                                            2964


<210>  4891
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4891
auggcaagaa uccuagcauu cgacaucggu auaaguucaa uaggcugggc cuuuucagaa       60

aaugacgaac ucaaagacug cggcgugaga auauucacaa aagcagaaaa cccuaaaaca      120

ggggaauccc uagcauuacc aagacgacua gcccgauccg cucgaaaaag gcuagcaaga      180

agaaaagccc gacuaaauca cuuaaaacau cuaauagcaa acgaauuuaa acuaaacuac      240

gaagauuacc aaucauucga cgaaagucua gcaaaagcau acaaaggaag ccucauaagc      300

ccauaugaac ugagauuccg cgcacucaac gaacuucuau caaagcaaga cuucgcuagg      360

gugauacugc acauagcuaa acgaagaggu uacgaugaua ucaaaaacuc agacgauaaa      420

gaaaaaggcg caauauuaaa agcuauuaaa caaaacgaag aaaaauuagc aaacuaccaa      480

agcgucggag aauaccucua uaaagaauac uuucagaaau ucaaagaaaa uucaaaagaa      540

uuuacaaaug uucgaaauaa gaaagaauca uacgaacgcu gcauagcaca aucauuccuu      600

aaagacgaac ucaaacuaau auuuaaaaaa caaagagaau uugguuucuc auuuucuaaa      660

aaauucgaag aagaagucuu aucaguugca uuuuacaaac gcgcauuaaa agauuuuagu      720

caccuaguag gaaauugcag uuucuuuacg gacgaaaaaa gagccccaaa aaacagucca      780

cuagcauuca uguuuguagc auuaacaagg auaauuaacc uucuaaacaa cuuaaaaaau      840

acugaaggga uacuauacac uaaagacgac cuaaauacac uauuaaauga aguucuaaaa      900

aauggaacac ucacauauaa acaaaccaaa aaacuucuag gccuaucaga ugauuacgaa      960

uucaaaggag aaaaaggcac auauuuuaua gaguucaaaa aauacaaaga auucauaaaa     1020

gcguuaggcg aacacaauuu aucgcaagac gaccuaaacg aaaucgcaaa agacauaaca     1080

cuaauaaaag augaaauaaa auuaaaaaaa gcgcuagcaa aauacgaucu gaaucaaaac     1140

caaauagacu cacucucuaa acuagaauuu aaagaccacu uaaacaucuc auuuaaggca     1200

cuuaaauuag ucacaccacu aaugcuagaa ggcaaaaaau augaugaagc uuguaacgaa     1260

cucaaccuua aaguagcuau caacgaagau aaaaaagauu ucuuaccagc cuucaaugaa     1320

acuuacuaua aagacgaagu aaccaaccca guuguacuac gugcaauuaa agaauacaga     1380

aaaguacuaa acgcacuacu aaaaaaauau ggcaaaguac acaaaauaaa uauagaacuc     1440

gcacgugaag uagguaaaaa ucacucgcaa agagcuaaaa uagaaaaaga acaaaacgaa     1500

aauuacaaag caaaaaaaga ugcagaauua gaaugcgaaa aacuagguuu aaaaauaaac     1560

ucgaaaaaca uacuaaaacu aagacuauuu aaagaacaaa aagaauuuug cgcauauagu     1620

ggcgaaaaaa uaaaaauaag cgacuuacaa gacgaaaaaa uguuagaaau agaucauauc     1680

uaccccuauu caagaucauu ugacgacuca uauaugaaua aaguucuagu auuuacaaaa     1740

caaaaucagg aaaaacuaaa ccaaacacca uuugaagcau ucgguaacga cagcgcaaaa     1800

uggcagaaaa uugaaguacu cgcaaaaaac uuaccaacaa aaaaacaaaa acgaauacua     1860

gacaaaaacu acaaagacaa agaacaaaag aacuucaaag auagaaauuu gaaugacacc     1920

agauauauag caagguuagu auuaaacuac acaaaagauu accuagacuu cuuaccacua     1980

ucagacgacg aaaauacuaa acucaacgac acacagaagg gaucgaaagu acauguagaa     2040

gcaaaaagug ggaugcuaac auccgcacug cgacacacgu ggggauuuuc ggcgaaagac     2100

aggaauaacc auuuacacca cgccauagac gcgguaauaa ucgcuuacgc aaauaacagc     2160

auuguaaaag cauucuccga uuucaaaaaa gagcaagaaa gcaacuccgc ugaauuauac     2220

gcaaaaaaaa uuucagaacu agauuacaaa aacaaaagga aauuuuucga gccuuucagc     2280

ggauuuaggc aaaaagucuu agacaaaauu gacgagaucu uuguuucuaa accugaaaga     2340

aaaaaaccaa gcggagccuu acaugaagaa acauuccgaa aagaagaaga auuuuaccaa     2400

uccuacggag gaaaagaagg cgucuuaaag gcacuagagc uaggaaagau aaggaaaguc     2460

aaugggaaaa uaguaaaaaa cggugauaug uuccgaguag auauauucaa acacaaaaaa     2520

acaaauaagu ucuaugcagu accuauauac acaauggacu ucgcuuuaaa aguccucccu     2580

aacaaagcgg uagcacgcuc aaaaaaagga gaaauuaaag auuggauacu aauggacgaa     2640

aacuacgaau uuugcuuuag ucuauacaaa gauucacuaa uucuaauaca aacaaaagau     2700

augcaggaac cagaacuagu auauuucaac gcauucaccu caaguacagu aagccuaauc     2760

guauccaaac acgauaauaa auucgaaacc cuaagcaaga aucaaaaaau acuauucaaa     2820

aaugccaaug aaaaagaagu uauagcuaaa ucaauuggca uacaaaaucu aaaaguuuuu     2880

gaaaaauaca uuguaagcgc ccugggagaa gucacuaagg cagaauuccg acaacgagaa     2940

gacuucaaaa augaacuaag auga                                            2964


<210>  4892
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4892
auggcaagaa uauuagcauu cgacauugga auaagcagua uugggugggc guuuucggaa       60

aacgaugaau uaaaagauug cggaguaaga aucuuuacga aagcagaaaa uccaaaaaca      120

ggagaaagcc ucgccuuacc ucgacgacua gcuagaucag caagaaaaag acuagcaaga      180

cgaaaagcac ggcuuaacca ucuaaaacac cuaaucgcaa augaauuuaa acuaaauuac      240

gaagacuacc aaaguuucga cgagagcuua gcuaaagcau auaagggcag ucucauaagu      300

cccuacgaac uacgauuccg agcacuaaau gaacuacuau ccaaacaaga cuuugcgaga      360

guaauacuac auauagcaaa gcgucgagga uacgaugaua uaaagaacuc cgaugacaaa      420

gaaaaaggag caaucuuaaa agcaaucaag caaaacgaag aaaagcuagc gaacuaccaa      480

ucaguaggag aauaccucua caaagaauau uuucaaaaau ucaaagaaaa uaguaaagag      540

uucacgaacg uacguaacaa aaaggaauca uacgaaagau gcauagcgca auccuuccuu      600

aaagaugaau uaaaacucau auucaagaaa caaagagaau uuggauucuc auuuuccaaa      660

aaguuugaag aagaaguacu uagcguagca uucuacaaaa gagcauugaa agauuucuca      720

caucuaguag ggaauuguuc auucuuuaca gacgaaaaac gcgccccgaa aaauucacca      780

cuagcauuua uguuugucgc acuaacaaga auaauaaacu uauuaaacaa ccuaaagaac      840

acagaaggua uauuauauac caaagacgac uuaaacgcac uauuaaauga aguucucaaa      900

aacuccaccc uaaccuacaa acaaacaaag aaacuacuag guuuaagcga cgacuacgag      960

uucaaaggcg aaaaaggcac auauuucaua gaauuuaaga aauauaaaga auuuaucaaa     1020

gcacucggcg accacucccu aagccaagac gaccuaaacg aaaucgcaaa agacauuacc     1080

uugauuaaag augaaaucaa auuaaagaaa gcacuugcaa aauacgacuu aaaccaaaac     1140

caaaucgauu cucuuucaaa auuggaauuu aaagaucauu uaaauauauc auucaaagca     1200

uuaaaacuua uaacaccacu aauguuagaa gggaagaaau acgacgaagc auguaacgaa     1260

uuaaacuuaa aaguagcaau aaacgaagac aagaaagacu ucuuaccagc auucaaugaa     1320

acguauuaua aagacgaagu cacaaaccca guaguauuac gcgcaauaaa agaauacaga     1380

aaaguauuaa augcacuacu aaagaaauac ggaaaaguac acaaaauuaa caucgaacua     1440

gcuagagaag uagggaaaaa cuacagucaa agagcgaaaa ucgaaaaaga acaaaaugaa     1500

aauuacaaag ccaagaaaga cgcagaacua gaaugugaga aauuaggauu aaaaauuaac     1560

ucuaaaaaca uucugaaacu gcgccuauuu aaagaacaaa aagaguucug ugcauacagc     1620

ggagagaaaa uaaaaauaag ugaccuacaa gaugaaaaga uguuagaaau agaccacaua     1680

uacccauaua guagguccuu cgaugauagc uacaugaaua aaguacucgu auucacuaaa     1740

caaaaccaag aaaaacuaaa uaaaacacca uucgaagccu ucggcaauga uucagccaaa     1800

uggcagaaaa ucgaaguauu agccaaaaac cuaccaacua agaaacaaaa acgaauauua     1860

gacaaaaauu auaaagacaa agaacaaaaa gacuuuaaag acagaaauuu aaacgacacc     1920

agauacauag cacgacuugu acuaaauuau accaaagauu accuagacuu ccuaccucuc     1980

ucagaugaug aaaacaccaa acuuaaugac acucaaaaag gcucaaaagu acacguagaa     2040

gcaaaauccg guaugcuaac cagugccuua agacauacuu gggguuuuuc ugcaaaagac     2100

aggaacaauc accuacacca ugcuauagac gcaguaauaa uagcauaugc gaacaacagc     2160

auugucaaag cauuuucuga cuucaagaaa gaacaagaaa gcaauucagc agaacuauac     2220

gccaagaaaa ucagugaacu ugacuacaaa aacaaaagaa aauuuuucga acccuucagc     2280

ggguucagac aaaaaguacu agacaaaaua gaugaaauau uuguaagcaa accggaaaga     2340

aagaaaccgu ccggagcacu acacgaagaa acauuuagaa aagaagaaga guucuaccaa     2400

ucauacggag gcaaagaagg agucuuaaaa gcacuagaau uaggaaaaau cagaaaagua     2460

aauggcaaaa ucgucaaaaa uggagauaug uuccgaguag acaucuuuaa acauaagaaa     2520

acuaacaaau ucuaugcagu accaauauac accauggauu ucgccuuaaa aguauuacca     2580

aauaaagcag uagcaagauc aaagaaagga gaaauaaaag auuggauacu aauggaugaa     2640

aauuaugaau uuugcuuuuc ucucuauaaa gauagccuaa uucugauuca aacaaaagau     2700

augcaagaac cagagcuagu cuacuucaau gcauuuacau ccucaacagu uucacugaua     2760

guaagcaaac augacaacaa auuugaaacg cuaagcaaaa accagaaaau acuauuuaaa     2820

aacgcaaaug aaaaagaagu aauagcaaaa ucaauaggaa uacaaaaucu caaaguauuc     2880

gaaaaauaca uaguaagcgc auuaggcgaa guaaccaaag cagaguucag acaaagagaa     2940

gauuucaaaa acgaacuaag auga                                            2964


<210>  4893
<211>  2964
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4893
auggcuagaa uauuagcauu cgacaucgga auauccucua ucgggugggc auuuucagaa       60

aaugaugaac uuaaagauug cggaguaaga auauucacua aagcagaaaa uccaaaaaca      120

ggagaaucac uggcacuacc ucgcagacua gccagaagug cacgaaaacg acuagcacgu      180

agaaaagcac gccuaaacca ucuaaaacac uugauagcua acgaguuuaa acuuaauuac      240

gaagacuauc aaagcuuuga cgaaucccuc gcgaaagcuu acaaaggauc auuaauuuca      300

ccauacgaac ucagauucag agcacuaaac gaauuacuaa guaaacaaga uuuugccaga      360

guaauccuac auauugcaaa acguagaggu uacgacgaca uaaagaauuc agacgacaaa      420

gaaaaaggcg caauauuaaa ggcuauaaaa caaaacgaag aaaaauuagc uaauuaccaa      480

ucaguaggcg aauaccucua caaagaguau uuucaaaaau ucaaagaaaa uuccaaggag      540

uuuaccaaug ucagaaauaa gaaagaguca uacgaaagau gcauagcaca aaguuuucua      600

aaagacgaac uaaaguuaau auucaaaaaa caaagagaau ucggauuuuc uuucucaaaa      660

aaauucgaag aagaaguauu aucaguagca uucuauaaac gagcauuaaa agacuucuca      720

caccuaguag gaaacuguuc auucuucaca gaugaaaaga gagcaccaaa aaacucacca      780

cuagcauuca uguucguugc ccuaacccga auaauuaacu uauuaaauaa ucucaaaaau      840

acagaaggga uacuauacac uaaagaugau uuaaacgcgc uacuaaacga aguucuuaaa      900

aauggaacgc uaacauauaa acaaacaaaa aaacuacucg gguuaucaga cgacuacgaa      960

uuuaaaggag aaaaaggaac auacuuuauc gaguuuaaaa aauacaaaga auuuauaaaa     1020

gcucuaggag accacagcuu aagucaagac gaucuaaaug aaauagccaa agacauaacg     1080

uuaauaaaag acgaaaucaa acuaaaaaaa gcgcucgcaa aauacgaccu aaaccaaaac     1140

caaauagacu cauuaaguaa auuagaauuc aaagaucacc uaaacauuuc auuuaaagca     1200

cuaaaacuaa uaacaccacu uaugcuugaa ggaaaaaagu augaugaagc augcaacgaa     1260

cucaaccuga aaguagcaau aaacgaagac aaaaaagacu ucuuaccggc uuucaaugaa     1320

acguacuaca aagaugaagu gacgaaccca gucguuuuaa gagccauaaa agaauacaga     1380

aaaguauuaa augcuuuauu aaaaaaauau gguaaaguuc acaaaauaaa uaucgaauua     1440

gcgagagagg uaggaaaaaa uuacagccaa agagccaaaa uagaaaaaga acaaaacgaa     1500

aacuacaaag caaaaaaaga cgcugaacuu gaaugcgaaa aacugggccu caaaauaaac     1560

ucaaaaaaca uccuaaaacu cagacuuuuc aaagagcaga aagaauuuug cgcuuacuca     1620

ggugaaaaga uaaaaaucag cgauuuacaa gacgaaaaaa uguuagaaau agaucacauc     1680

uaccccuacu cacgaucauu cgacgauucu uauaugaaua aaguuuuagu uuucaccaaa     1740

caaaaccaag aaaaauuaaa ccaaacuccc uucgaagcgu ucggaaacga uagugccaaa     1800

uggcaaaaaa uagaaguucu agccaaaaau cuaccuacca agaaacaaaa gagaauauug     1860

gacaaaaacu auaaagacaa agagcaaaaa gauuuuaaag acagaaaucu aaaugauaca     1920

agauacauag cacgacuagu auuaaacuau accaaagacu acuuagauuu ccuaccgcua     1980

agugacgacg aaaauacaaa acuaaacgau acacaaaaag guucaaaagu acauguggaa     2040

gccaaaucug gaaugcuaac aagcgcuuua agacauacuu ggggauucag ugcaaaagau     2100

agaaauaacc accugcauca ugccauagau gcaguaauaa uugcauacgc aaauaauucu     2160

auuguaaaag ccuuuagcga uuucaaaaaa gaacaagaaa gcaauagugc cgaauuguau     2220

gcaaaaaaaa uuuccgaacu agauuauaaa aauaaaagaa aauuuuuuga accuuuuagc     2280

ggauuuaggc aaaaaguauu agacaaaauu gaugaaauau uuguaucuaa acccgaaaga     2340

aaaaaaccaa guggugcccu acacgaagaa acauucagaa aagaagaaga auucuaucaa     2400

agcuacggag gaaaagaagg cguacuaaaa gcauuagaac ucggcaaaau uagaaaagua     2460

aaugguaaaa ucguaaaaaa uggagauaug uuccgcguag acauauuuaa acacaaaaaa     2520

acaaacaaau ucuacgccgu gccuaucuac acaauggacu ucgcccuuaa aguucuacca     2580

aacaaagcag ucgcgagauc uaaaaaaggc gaaauaaaag auuggauauu aauggaugaa     2640

aauuaugaau uuugcuucuc acucuacaaa gauucacuua uccuuauuca aacaaaagau     2700

augcaggaac cagaauuugu auacuacaau gcauuuacaa gcucuaccgu cucauuaauc     2760

guaaguaaac augauaacaa auucgaaacc uugagcaaaa accaaaagau acuauucaaa     2820

aacgcaaaug aaaaagaagu aauagcaaag ucuauaggaa uccagaacuu aaaagucuuu     2880

gaaaaguaua ucgugucagc auuaggugaa guaacuaaag cagaauucag gcaacgggaa     2940

gacuucaaaa acgaacuacg auga                                            2964


<210>  4894
<211>  2979
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4894
augagaauau uuagcgaugg gagaaaucca aaagauggau caagccuugc cguaacuaga       60

agggcagcua gagcaaugag aagaagaaga gaccgucuuu uaaaaagaaa aacaagaaug      120

caagcaaagc uaguagaaca uggauucuuu ccugcugaug caggaaaaag gaaagccuua      180

gagcaacuca auccauaugc auuaagggca aaaggauuac aagaagcauu guuaccaggu      240

gaauuugcac gggccuuauu ucauauaaau caaagaagag gauuuaaauc aaauagaaaa      300

acagauaaga aagauaauga cucuggagug uuaaagaaag cuauugguca auuaagacaa      360

caaauggcag aacaaggaag ucguacugua ggagaauauc uauggacuag acuacaacaa      420

ggucaaggcg uaagagcacg guacagagaa aaaccauaua cgacugaaga aggaaagaaa      480

cgaauagaca aaucauauga cuuauauaua gauagagcaa ugauagaaca agaauuugau      540

gcacuauggg ccgcgcaggc agcauucaac ccuacauugu uucaugaggc agcaagagca      600

gacuuaaaag acacauuacu acaucaaagg ccacuuagac caguaaaacc aggaagaugu      660

acacuccuac cagaagaaga aagagcacca cuagcgcuuc cgaguacaca aagauuccga      720

auacaccaag aagucaauca cuuacgacuu cuagaugaaa aucuaagaga aguggcacua      780

acucuagcgc aacgagacgc gguuguuaca gcucuugaga cuaaagcuaa auuaucauuc      840

gaacaaauuc gaaaauuguu aaaauuaagu ggaaguguac aguucaaucu ugaagacgcg      900

aaaaggacag aacuaaaagg uaaugcaacu agcgcagcuu uagccagaaa agaauuauuc      960

ggcgcugcuu ggaguggauu ugaugaagcu cuacaagacg aaauaguaug gcaguuagug     1020

acugaggaag gagaaggagc gcuuauagcg uggcugcaga cucacacggg ugucgacgaa     1080

gcaagagcac aagcaauagu ggacguguca uuaccagaag gauaugggaa uuuauccaga     1140

aaggcuuuag caaggauagu acccgcauua cgagcugcug uaauaacgua ugauaaagcu     1200

guacaggcug cagguuuuga ucaccauagc caacuaggau uugaauauga cgccucagaa     1260

guggaagauu uaguacaucc agaaacagga gaaauccgaa guguauuuaa acaacuacca     1320

uauuauggaa aagcacuaca aaggcauguc gcauucgguu caggcaagcc agaggauccg     1380

gaugagaaaa gauaugggaa aauagcuaac ccaacaguac acauaggucu gaaccaaguc     1440

agaaugguag uaaaugcguu aauaagaaga uaugguagac cgacggaagu gguaaucgaa     1500

uuagcaagag accuuaaaca aagucgggaa caaaaagucg aagcccaaag aagacaagca     1560

gauaaucaaa gacggaaugc aagaauuaga agaaguauag cagaaguauu agguauagga     1620

gaagaaagag uaagaggaag ugauauacaa aaguggauau gcugggagga acucuccuuc     1680

gacgcagcag auagaagaug cccauauagu ggaguacaaa uaagcgcggc uaugcuacuc     1740

ucugacgagg uugaaguaga acauauauua ccuuucucaa aaacgcuaga cgauagccuc     1800

aacaauagaa caguagcuau gaggcaagcg aauagaauca aaagaaauag aacaccaugg     1860

gaugcaaggg cagaauuuga agcacaagga ugguccuacg aagauauauu acaaagagca     1920

gagagaaugc cauuacgaaa acgauaucgc uuugccccug augguuauga aaggugguua     1980

ggagacgaca aagauuuccu ugcacgagcu uuaaaugaua ccagguauuu aaguagagua     2040

gcagcggaau accugcgacu uguaugcccg ggaacaagag ucauaccagg acaauuaacu     2100

gcacuauuga gaggaaaauu uggcuugaau gaugucuuag gcuuagaugg ugagaaaaau     2160

agaaaugacc aucgacauca cgcaguagac gcguguguaa uaggaguuac cgaucaagga     2220

uuaaugcaac gcuucgcaac agcgucagca caagcuaggg gagacggcuu aacgcgacug     2280

guggacggua ugcccaugcc guggccaaca uacagagauc acguugaaag agcaguacgu     2340

cacauauggg uuagucauag accagaucau gguuuugaag gugcuaugau ggaagaaacu     2400

ucguauggua uaagaaaaga cgggagcaua aaacaaagaa gaaaagcaga cggaucagcu     2460

gguagagaaa uaaguaaucu aauuagaaua cacgaagcca cacaaccguu acgucacgga     2520

guuagcgcgg acggacaacc ccuugcauau aaaggauacg uaggaggaag caauuacugc     2580

auugaaauaa cagugaauga caagggcaaa ugggagggcg aaguuauuuc aacauuuaga     2640

gccuacggug uaguacgugc aggagggaug ggaagauuac gaaauccuca ugaaggucaa     2700

aacgguagaa aacuuauaau gagauuaguc auaggggaua guguccgauu ggaaguagau     2760

ggugcagaaa gaaccaugag aauaguaaaa auaucaggua guaacgggca aauauucaug     2820

gcacccauac acgaagcuaa cguagacgcc cgaaauacgg acaagcaaga ugcauucaca     2880

uacaccagua aguacgcagg aaguuuacaa aaagcgaaga caagaagggu uaccauauca     2940

ccaauaggcg aagugagaga uccagguuuu aaaggauga                            2979


<210>  4895
<211>  2985
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4895
augacucucg gauuagacuu aggaacgaac agcauaggau gggcauugau agauuuugac       60

gauaaccaac aaccgagaaa ucugauagcu ugcggaucaa gaauauuuca agaagcugua      120

gaagcaaaaa cagguacccc caaaaaucau gcaagaagaa cagccagagc agcucgcaaa      180

uuaguugcuc ggagaaaaca aagacgaaca aaacuauuaa accugcuaau aaggcauggc      240

uuauuaccuc aagacguugc agaaagaaau gcacuauuau uagacaacca ggcuaacaac      300

cccuaccaau uacggaaacg aggauuagau gaaaaacuug aaccuuuugc uuuugguaga      360

gccuuauauc aucucgcuca ucgaagaggg uuucaaucca aucgaaaggc agcuagugau      420

gaagauggga aagucaaaac ggcgauuuca acuuuaagag ccgaaaugca aggaagaaca      480

uuaggagccu uuuuggcaga ccaaccaacg caaaggcgac gauauacuga cagggcaaug      540

uacguagaag aauuugaaca acuauggagu aagcaggcaa guuuuaccca agaacaauua      600

acacaggcac ucaaaguagc aauacauaau ucuaucuucu uccaaagacc auuaaaauca      660

caaaaacacc uaguagguaa gugcacauuu gaaccgacua gaaaaagagc cgcaaaagca      720

auacuuuuaa cccaaagauu uagaauuuua caagacguaa aucauuuagc aguaaagaac      780

ccaauuacca gagaauuuag accauuaacu caagaugaac ggaacaaauu agccgaagca      840

cuugacaaac agaaauccau gacaugggga gcgguucgaa aaguaauagg uuggaauaag      900

aaagauucgu uacaugaagg ugaaacauuu aauuuggaag aggguaagaa agacaaauua      960

auaggaaaua gaacggcaug ugaccucaga ggagcucucg gagauagaug ggacgcuuua     1020

acugcacaac aacaggacga ucuaguaaca gacuuguuga caauagacaa cacggaugga     1080

uuuuuaaaaa gaaugaaaac acacugggaa uuugacgacg caacagccga aguaauugca     1140

aaaauagaau uagaaccugg cuaugcgaga uuaucagcaa aagcaaugag ggcaauacug     1200

ccauaucuag aacaaggaau gauuuacuca gacgcaugca aagcugccgg auaugaccac     1260

ucaaauccaa aucaaagaac gaucacagac aaauuaggac uaccaucaaa uguaagaaac     1320

cccguaguac agaaagcacu uuuugaaacu agaaaaguag uaaaugccau aguuagaaaa     1380

uauggcaaac cagcaguaau aagaguugaa auggcaagag auaugaaacu gucaaagcgc     1440

cagaaagaag aacuccaaaa agaacaaaau aaacaaaaga aagcuaauga auuagcagaa     1500

ggaauuuuaa gaaaagaguu cggaauacag caaccagcua gagcagacau ucaaaaauac     1560

aacuuguggu uagaauguaa agaaguuugc ccuuauacag guacaaccau uucaagagaa     1620

augcuauuuu caccagaagu ugaugucgaa cauauacuac cauauagucg aucccuugau     1680

gauucuuaua ugaauaaaac acuaugcaug gccaccuuca acaggcaggu uaaacuaaau     1740

aacacaccuu acgagauugg gcaaggagau ccauuagcuu augcagaaau auuucaaaga     1800

auaaaaacau ugccauuucc aaaacgaaga agauuugaac aaaaagaaau agaaacagau     1860

aaauucauag aacgacaacu aaaugauacu agguauauau gcguagaagu acgagacuuu     1920

cuacaacaau ugggagcacc aguagaaguc ucaaagggag aagcuacugc gaaauuaaga     1980

ggaagaugga aauuaaacac acuauuaagc ccagauggau caaacgaaaa auauagagga     2040

gaucacagac aucaugcaau agaugcaguu guuauugcac ucacuaaucg gagucucuuu     2100

cauaaauuga guagauuauc agcacaauca auaaguuuag acaaaagcag auuugacgua     2160

cccccacccu gggacggauu uuaugacgac guauacgaga aaaucgacaa aguaauagua     2220

ucacacgcac cagcuagaaa aauaggugga gcucuccaug aagacacugc guauggucau     2280

uuuaaagaag aaguagcuga ucccaaaaca ggaaaaguaa guauagagga cagauucgua     2340

uauagaaaac cauuagccac gcucacagca aacgaaguug guaaaauaag agacgcaaaa     2400

guccgagaau uugucgaagc ucgacucgcc caauucggaa auaacucaaa aacagccuuc     2460

ggagauccaa auaacccauu acuacacgca gacgguaaaa cgccaauacg aacaguuaga     2520

uuaguaacaa auuuuaacaa agccaccaca caugcaauac aaaaaggcca agguagagac     2580

uacaaauuuu ucaaauacgg uaacaaucau cauguagaga uuauagaaca cguugaaacc     2640

ggaaaacgaa aaggaaucuu cguaacagca auggaagcag caagaagagu acgcggagua     2700

gcuaauacag aaaagucaga uaucguccaa agaaaucacg gagaggaaug gagauucgua     2760

augucuuuau caauaaauga ccuuguaagu auucaggaag cugaucaagu cagaauaaug     2820

agaguacaau uguuugaugc cucaaauggg aauauaauau uuagagauca aaaaucagca     2880

augcuagaug acaagcuauc acgcuaucaa acgaaagccc aucugcuagu augucagaaa     2940

auuaccaccg auccacuagg aaaucuagca ccuuguaaug auuga                     2985


<210>  4896
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4896
augucaaaag uucuaggacu agauauagga gcaaacagca uaggaugggc ccuuaucgau       60

gacgaagaaa acgauauaau aggugcaggg guacggguau ucaaagaagg agucaauuua      120

aagggaguaa aagaagaaag uaaaaaugua gaaagaagag cagcaagguu agcccgaaga      180

caacauaaaa gacgaagcca aagacggaau agacuagucg cacuacuaaa aacccuugaa      240

auguuuccag agaauaacga agauauuuca gauuacuacg cacaagaccc auaugaacua      300

agaaaaaaag gcuuauacga aaaacuaaca auguaugaau ucgguagagu auuauuacac      360

auaugcagaa gaagagguuu uaaaagcagc cgaaaaacag auauaucaga agaagguaaa      420

aucuuugaag gaaaagacgg aauaguagga auaaaucaaa caagagacag cauaagggag      480

gguggcuuca aaaccaucgg ugaauacuua gcaucacuug auccacaugu uauaagaagg      540

agaaacagau ucacacuacg agauauguac gaagaagaau ucaaccuaau gugggagaaa      600

caacaaucau uuuauccccu ccaauuaucu aacgaauuca aagaagccau agcagaaguu      660

auucuaaguc aaagaaaacu aaaaucacag aaagcgcugg ugggcaauug cggauuagaa      720

ccuaauaaaa aaacagcacc gaaaaguucu ccugcauuuc aguacuuucg gauauuagag      780

caacuaagca gaauucuuau aucgauacca cacacgggua gguacaauga cccuuuaaac      840

gaagaagaaa ggagaauguu aaucgacaaa cugucaguca cagaaaaacu caaauucagu      900

gacaugagaa aaaugcuaaa ccuaccggga gaaucugaca uuaauauaga ggaagaaggu      960

ggaagacuac uuggcaacac cacauacgca aggcuaauaa aaguuuucgg aaaaaaaaga     1020

ugggcagguu uuucagaaga agaaaaagaa caaauuuggc acacauucca uuucgcagau     1080

aaccaugaau ggcuaaaaga auaugcauua aaaaaguggu caauaacacc agacgagauu     1140

aaagaacuug gaaaaguggc auuagaaaaa gaauaugcgc gauuuucuaa aaaaucacug     1200

aaaaaaauaa uaccccaacu agaagaaggg cuaacgcuag auaaagcuaa aauagaagca     1260

gguuacacuc cagaauauga aauagagaaa agccuaagug cguacuuacc auuaccagaa     1320

aauauccgaa acccaaucgu acaacaagca cucuacgaau uaagacacgu agugaaugcg     1380

gucauaagaa cauacgguaa accagauaua gcuagaguag aacuggcaag agaaaugaaa     1440

aaacccaaac aaaaacguga aaaaaugaga ucagaguuaa gaaaacgcca aaugaaauuu     1500

gaagaaauaa aaucaauucu aaaagaaaga uuaggauuua uagacccaaa aagagacgaa     1560

auucauaaau auauguuaug ggaggaaugu cgcgaaacau guccauauac uggcagaaaa     1620

auaucucucu cggcccuaua cgguggagau uuugaaguag aacauauaau acccuauaaa     1680

agaucccuag augauucauu auuuaacaaa acugugugcu uaagagaaga aaaccaaaua     1740

aagggagaua gaacaccaua cgaggcauac aguggggacc gucagaaaua ugaagaaauu     1800

auuuuaagag uccaaaaaac gucaaugccu cgaggaaaaa aaaaccuuuu ugcaaucaaa     1860

gaauuaaaag augacuucgc acacagacag cuauccgaca cagcauauau cucacgcgaa     1920

acacgaaaau accuuggcag cacaauaaaa aaagugcaag cgguuaaagg uggcagcaca     1980

gcaaaauuac gauacuacug gggucuaaau aguauacugg gaguacgaga cauaaaaaug     2040

agagacgacc auagacauca ugcaguggac gcucuuguca uagcaaauac aacuuaugcu     2100

uuuguuaaua cgcucagccg auaucacucg auggauguag aaccucauga aucuagauuu     2160

ccauugccau gggaacaauu uagaaaugau gcugaaagcg uaguaagaaa uauauucgug     2220

agccaccguc uuggaaaaaa agcaacggga caauuacaug aagaaacaua uuacgguaaa     2280

auaagaaaca ccgauggaga gccauauuuc gucguuagga aaaaacuagg uacguuaaca     2340

gccaaacaaa uagcaaauau uguugauccg guaguaaaga cacaaguguu cgaaagacua     2400

gaagaaaaag gaaucgacau cgaaucuuca aacaaaauac cagcagaugc uuuuguagaa     2460

accuuauaca ugccagauag gaaaacaccu auuaaaucag uaagaguuaa aguaccauca     2520

acaacuauga uacaacucua caaggacaaa gaacuauaug uggacccagg uucaaaucac     2580

cacaucguaa uauucgaaaa ugaggaagga aaacgagaug gcauaguagu gucacuauuu     2640

gaagcagcac aaagacuaag aagaaacgaa ccugucauac aaaaaacaug gaaaccagaa     2700

uggcauuucg uaaugagucu aucaauagau gaacuuauau uacucgacgu agaugagaag     2760

aauaucgauu ggggcgauac aggauuagcu uugcgauacu cgaaagcauu auauagaguu     2820

caaaaaaugg acguaaauaa aauaauaacu cugagacacc aucucgucgc uagguuaaaa     2880

gaugacgaag gaaaagaauu cggaagagaa cacagaauac cuagcacacu gaggggucua     2940

aaagucaaaa uugaucauau uggaagacua agaagggcgu augacuga                  2988


<210>  4897
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4897
augaaaguaa uaggauucga cauuggaaua aauuccaucg gaugggcauu cguagaaaac       60

gacgaacuua aagacugcgg ugugagaaua uucaccaaag cagaaaaucc caaaaacaaa      120

gaaucacuag cguuaccgag aagaaacgcc agguccagua ggaaaagacu aaaacgaaga      180

aaacuccgcc uaauugcaau gaaacacauc cucgccaaag aacuuaauuu aaacuacaaa      240

gacuacauag caaaugaugg cgaacucccc aaagccuacg aaggaaaacu caucaguccc      300

uacgaacuca gguauaaagc acucaaucaa aaacuggaag cgaaagacuu agucagagua      360

auacuacaca uagcaaaaca ccguggauac augaacaaaa augauaaaaa aaguaaugac      420

gccgaaaaag guaaaauauu gucggcacua aaaaauaacg cacuaaaacu agaaaaauac      480

cagucaguag gagaauauuu cuacaaagaa uucuuccaaa aauacaaaga aaauacuaaa      540

gacuucauaa aaauaagaaa cauaaaagac aacuacaaua acugcguauu agccagugac      600

cuagaaaaag aauuaaaacu aauacucgaa aaacaaaaag aauucggaua cagcuacuca      660

aaugacuuua uagaagaagu aauacaagua gcauuuuucc aacgaccucu aaaagauuuc      720

agccacuuag uaggaaccug cacauuuuuu gaagaagaaa aacgagcaug caaaaacagc      780

uacucagcau gggaauuugu agcucucaca aaaauuguaa augaacucaa aucauuagaa      840

aaaauaucag gagaacuuau aucaacucaa acaauaaacg aaauacuaaa cuacauacuc      900

gacaaaggaa gcauaacaua uaaaaaauuu agaucauaua uaaaccucua ugaaaguaua      960

aaauuuaaaa gcuuaaaaua cgacaaagau aaugcagaaa gugcaaaauu gauagauuuc     1020

agaaaacuag uugaauuuaa aaaagcucua ggugaacacu acuuuaacag acaagaacuu     1080

gaucaaauuu caacuuacau aacacuaaua aaagacaaug uaaaacuaaa aacagcauua     1140

gaaaaauaca aucuaucaaa ugaacaaaua aacaacuuac uggaaauaga auucaacgau     1200

cacauaaauc uuuccuucaa agcauuaucg augauuuuac cccuuaugaa agaaggaaaa     1260

aaauaugaug aagcauguga aauagccaau uuaaaaccga aaaccacaaa ccaaaaacaa     1320

gauuuuuugc cagcauucug uaacucaaua uucguccacg aacuaucaaa uccaaucgua     1380

aacagagcua ucagcgaaua cagaaaagua cuaaacgcac uacuuaaaaa auacggaaaa     1440

guacacaaaa uacaccucga acucgcaaga gauaucgggc uauccaaaaa agccagagaa     1500

aaaauagaga aagaacaaaa agaaaaccaa gcaauaaaua caugggcccu aaaugaaugc     1560

gaaaacaugg gaauaaaagc aaacgcuaaa aacauacuaa aacuaaaacu auggaaagaa     1620

caaaaagaaa uaugcauaua uagcggcaag aaaauaucaa uagaacaucu aaaagacgag     1680

aaagcacuug aaguagacca uauauacccc uacucgagaa guuucgacga cucauacaug     1740

aacaaaguau uaguauucac caaggaaaac caagaaaaau ugaauaaaac accuuucgaa     1800

gcauucggaa aaaauaucga aaaauggaac aagauacaaa cacuagccca aauauuacca     1860

uauaaaaaga aaaacaaaau acuagaugaa aacuucaagg auaaucagca acaagacuuu     1920

auaaguagga accuaaauga uacuagauac auagcaaccc ucauagcuaa auacacaaaa     1980

gaauaccuua acuuucuacc auuaaaugaa aaugaaaaca uaaaucucaa aaguggagaa     2040

aagggaucaa aaauacacau acaaacaguc aaugggaugc uaacaucggu gcuccgccau     2100

acuuggggcu uugacaaaaa agauagaaac aaucaccuuc accaugcccu agacgcgauc     2160

auaguagcau acucuacuaa uucauuaauc aaggcauuca gugacuuuaa aaaaaaucaa     2220

gaguuacuua aagccaaacu auacgcaaaa gaacuuacaa acgacaauua cauccaucaa     2280

gcaaaauucu ucgaacccuu ugaaggcuuu agagaaaaga uacuaaacaa aauagaugag     2340

auauuuguau caaagccgcc acgaaaaaga gcacgaggag cccuacacaa agagacauuu     2400

uacucagaga aaaaaaugau aaaaaaauau aacuccaaag aaggacuaaa cauagcacua     2460

gacugcggaa aaauaagaaa aauaggaaca aaauacguag aaaaugauac aauggugaga     2520

guagacauau ucaaaaaaca aaacaaauuc uacgccaucc ccauauacac cauggacuuu     2580

gcacuaggga uacuccccaa caaaauuguc guaauuggca aagacaaaaa uaacaaucca     2640

aaacaauggc aagaaaucga ugaaucauac gaauucuguu uuucacuaua uaaaaaugau     2700

cuuauccuau uacaaaaaaa agauaugcaa gaagcagaau ucgccuacua uaacggauuc     2760

gacauugacu cuagucgaau aaacauaaag aaacacgaca auaaauucga aaaccucaca     2820

aguaaucaaa aacuacuauu cacaaacaaa gagaaaaaca ggacaggaau ucaaaaucuu     2880

aaaauauucg aaaaauauau cguuacaccc uuaggaaaca aaaucaaagc ugacuuucaa     2940

ccaagacaaa acauaaguuu aaaauccagc aaaaaaaaug gcuuauga                  2988


<210>  4898
<211>  2988
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4898
augaaaaucc ugggauuuga caucggaauu aacucaauag gcugggccuu cguagaaaac       60

gacgaauuaa aagauugugg cguacgaaua uucacaaaag cagaaaaccc aaaaaauaaa      120

gaaucacucg cacucccacg aagaaaugca cgaucaaacc gucgaagacu caaaagaaga      180

aaaagcagac ugauagcaau uaaacauaua cuugcaaaag aacuuaaucu aaauuauaaa      240

gacuauauag caaaugacgg agaacuacca aaagccuacg aagguaaauu aaccaauguu      300

uacgaacuaa gauauaaagc acucaaccaa aaacucgagg cuaaagauuu agcaagaguc      360

auucuacaca uagcaaaaca uagaggauac augaacaaaa augaaaaaaa aucgaaugac      420

agcgaaaaag gcaaaauacu cagcgcucua aaaaacaacg cacucaaacu agaaaaauau      480

caaucuguag gagaauacuu uuauaaagaa uuuuuccaaa aauacaaaga aaacacaaaa      540

aacuucauaa aaauacguaa cacaaaagac aacuauaaca auugcguacu cgcuucagau      600

cuugaaaaag aauuaaaacu aauacuagag aaacaaaaag aguucggaua uucauacaac      660

gaugacuuca ucaaagaaau cauaaacaua gcuuuuuucc aaagaccacu aaaagacuuu      720

ucacaccuag uuggaagcug cacauuuuuc gaagaagaaa aacgagcuug uaaaaacuca      780

uacucugccu gggaauuugu agccuuaagu aaaauaauca acgaauuaaa acgauuagaa      840

aaaauaucag gagaacuuau aucugcacaa acuauaaaug aaauacuuaa ccacauauua      900

gacaaaggaa acauaacgua caaaaaauuu agaucaugca uaaaucucca ugagagcaua      960

gaauucaaau cacuaaauua ugauaaagac aacgcagaau cagcaaaacu cguagacuuc     1020

aaaaaacuaa ucgaauucaa aaaagcacua ggcgaacacu cauuuaaccg acaagaacuu     1080

gaugaaaucg caacccauau cacccucauu aaagacaacg auaaacucaa aacagccuua     1140

gaaaaauaua aucuaaauaa cgagcaaaua aacgcacucc uagaaauaga auucaacgac     1200

cauauuaauc ucucuuucaa agcccuuaac uuaauauuac cccucaugag agaagguaaa     1260

agauacgacg aagccugcga aauagucaau cuaaaaagca aaacaaguaa ccagaaacaa     1320

gauuuacucc cugccuucug ugacuccauu uuuagucaag aacuguccaa uccuguggua     1380

aauagagcca uuucagaaua cagaaaagua uugaaugccc uacucaaaaa auacggaaaa     1440

guccacaaaa uacaccuaga gcucgcacga gauguaggac ucuccaaaaa agcacgugaa     1500

aaaauugaaa aagaacaaaa agaaaacaag gagauaaacg acugggcauu agacgaaugc     1560

gaaaaauuug gcuuaaaagc uaauaacaag aauauacuca aacuuaaacu auggaaagaa     1620

caaaaagaaa uuugcgcaua caguggcaag aaaauaucaa ucgaacaccu aaaagaugag     1680

aaagccuuag aaguggauca cauauaccca uacucaagga gcuucgacga cuccuacaug     1740

aacaaaguuc uaguuuuuac caaagaaaac caagaaaagc uaaacaagac acccuucgaa     1800

gcauuuggca aaaauaucga aagauggaac aaaauccaag cacuagccca aaaccugccc     1860

uacaaaaaaa aaaauaaaau acuugaugaa aacuucaaag auaaacaaca acaagacuuu     1920

auuucacgaa aucugaauga cacacgauau aucgcuaccu uaauugcaaa auacacuaaa     1980

gaauaccuua acuuucuacc gcucaaugaa aacgaaaaua caaaccucaa aucaggagaa     2040

aaaggaucca aaauacaugu acaaaccaua aacggaaugc ucaccuccgu uuuaagacau     2100

acuugggguu uugauaaaaa agaucgaaac aaucacuuac aucacgcacu ugacgcaaua     2160

aucguagccu auaguacaaa uucaauaaua aaagccuuuu cugauuuuaa aaaaaaccaa     2220

gaacuacuua aagcuaaacu uuacgcaaaa gaacuaacgu cagacaauua caaacaccaa     2280

gcaaaauucu ucgaaccuuu ugaaggauuu cgagaaaaaa uacuaaacaa aauagacgaa     2340

auauucguaa gcaaaccgcc gagaaagaga gcacgaggaa cauuacauaa agaaacauuu     2400

uacccagaag aaaccaugau aaagaaauac aacuccaaag agggacuaaa cauagcacuc     2460

aacugcggaa agauacgcaa aauaggcaca aaguacguag aaaaugacac aaugguaaga     2520

uuagacauau ucaaaaagca aaacaaauuc uaugcuauac caauauacac aauggauuuc     2580

gcacucggaa uacuccccaa uaaaauuguc aucgcaggca aggacaaaaa caauaaccca     2640

aaacaauggc aagaaauaga ugagaguuau gaguuuugcu uuagccucua uaaaaacgau     2700

cuaauacuuu uacaaaaaaa agacaugcaa gaagccgaau ucgcauauua uaauucauuc     2760

gacauagacu cgagucgaau caacaucaaa aagcaugaca acaaauucga aaaccuuaca     2820

gacaaccaaa aauuacuauu uacaaaucaa gaaaaaaaca gaacuggaau acaaaaccug     2880

aaaauuuucg aaaaauacau uguaacaccu uuaggagaca aaauaaaggc agacuuucag     2940

ccacgccaaa acauaagucu aaaaacaagu aagaaauaug guuuauga                  2988


<210>  4899
<211>  2991
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4899
augaugggua acuucacacu cggauuagau cuaggaacua acucaauagg augggcccua       60

auagauuuug augacaacca acaaccucaa aauuuaauag cuugcgggag ucgcauauuu      120

caagaagcag uagaagccaa aacuggcaca cccaaaaauc acgcaagacg agcagcccga      180

gccgccagaa aguuaguugc acgacgaaaa caaaggagga caaaauuacu aaauuuauua      240

guaugucacg accuccuucc acaagaugaa gcagaaagaa acgcacucuu acuagauaau      300

caagcaaaca auccauacca gcuaaggaaa aggggucuag augaaaaacu agaaccauuu      360

gcauuugggc gggccuuaua ccauuuagcc cacaggagag gauuucaaag uaauagaaaa      420

gccgcaucag augaagaugg aaaaguaaaa acagcaauuu cagcacuucg agcagaaaug      480

caaggccaca cuuuaggagc cuuucuagcu gaucaaccca cacaaagaag aagauauacc      540

gacagagcaa uguaugcgga agaauuugaa caacuauggu caaaacaaac aauauuugca      600

cccgaacaac uaacucaagc acuaaaggua gaaauuaaaa auaguauauu cuuccagcga      660

ccauugaaaa gucaaaaaca ucuaguaggc aaauguaccu uugaaccaac acgaaaaaga      720

gcagcaaaag caauauuacu cgcgcaacac uuucguauac ugcaggacgu aaaucauuua      780

gcaguuaaaa auccaauaac aagagaauuu cgaccucuga cacaagacga acgaaacaaa      840

uuagcagaag cauuagauaa acaaaaggga augaauuggu cugcaguaag aaaaguaaua      900

ggauggaaua agaaagauuc gcuacacgaa ggagaaacuu uuaaccucga agaggggaag      960

aaagauaaau uaauaggcaa cagaacagca ugcgaccuac guggagcguu aggagacaga     1020

ugggacgcuu ucacggcacc acaacaagac gacuuaguua cagauuuacu aaccauagau     1080

aacccugacg gauuccuuaa aagaaugaaa acacauuggg gcuuugacga cgcuacugca     1140

gaagcauuag cuacgacaga auuagagccu ggauaugcua ggcuaagcuc aaaagcgaug     1200

agggcaauuu uaccgcaucu agagcaagga augauauacu cagacgcaug uaaagccgca     1260

gguuaugauc acucaaaucc aaaucaaaga acaauaacag acaaauuggg cuuaccaucg     1320

aauguccgga auccaguagu acaaaaagca cuuuuugaga caagaaaagu aguaaaugcc     1380

auaguaagaa aauacggaaa accugcgguu auacgugucg aaauggccag agacaugaaa     1440

uuaucaaaac gacaaaaaga agaauuacaa aaagaacaaa auaaacaaaa gaaagcaaac     1500

gaacuagcag aaggaaucuu aagaaaagaa uuugguauac aacaaccagc aaggacugau     1560

auacaaaaau acaaucuaug guuagaaugu aaggagguuu gcccauauac aggcacaguu     1620

auaagcagag aaauguuaua uaguccagaa guagauguag aacacauauu accguauuca     1680

agaagucuag acgauucaua caugaacaaa acacuaugua uggccacaua uaaucgacaa     1740

acaaaauuaa auaauacacc uuacgaaaua ggucaaggag acccagucgc uuaugcagaa     1800

auauuucaga gaaucaagac acuaccauuc ccaaaaagaa gacgcuuuga acaaaaagaa     1860

auagagaccg acaaauuuau agaaagacaa uuaaacgaua cacgauacau augcguagaa     1920

guaagagacu uucuacaaca acuaggugcc ccaguagaag uaucaaaagg ugaagcgaca     1980

gcaaaauuaa gaggaagaug gaaauugaau acuuuauuau cacccggcgg ugguaacgaa     2040

aagaauagag cagaucauag acaucacgcu auagacgcag uuguuauagc ucuaacaaau     2100

cgauccuuau uccauaaauu aucacgacuc ucagcgcaga guauaucauu ugauaaaagc     2160

cguuucgacg uacccccacc cugggacgac uuuuaugacg auguauacga aaagauugau     2220

aaagucaucg ucucacacgc accuaccaga aaaauaagug gagcauuaca cgaagcuaca     2280

gcauauggac auuuuaaaga agaaauagca gacccgaaaa cagguaaauu cucuauugaa     2340

gacagauucg uuuacagaaa accucucacu acuuugacag caaaugaagu agguaaaauc     2400

agagaugcua aaguaaggga gcuaguagaa gcccgauuag cacaauucgg aaacaacuca     2460

aaagcugcau uuggagaucc gaacgagcca cugcuucaug ccgacggaaa aaccccaaua     2520

aggaccauaa gacuaacaac aaacuucaac aaaacuacaa cacacgcaau ccaaaaagga     2580

caagauagag auuauaaauu cuucaaguau ggaaauaauc aucacguaga aauaauagaa     2640

cauguugaaa cuggaaagag aaagggaaua uucgucaccg caauggaagc agcaaggaga     2700

gcaagaggug uggcaaauac ugaaaaaucu aauauagucc aacgaaauca cggcgacgga     2760

uggaaauuug uuauguccuu agccgucaau gacaugguac guacagaaga uacgcaagca     2820

auauauaggg uucaaaaacu agauggaagu aauaacagau uuacucuaag gagccauaac     2880

gcagccacac uugaugauaa acaaacaggc guagacaaga cuccaaaccu cuucaaaggu     2940

accaaaauaa uaguagaccc auuaggcaau cuuacacagu guaaugauug a              2991


<210>  4900
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4900
augaaaguuc uaguagaugc aggauuaaug cccagugauc cagcagcugc aaaagcauua       60

gaaacacuag accccuacga auuacgagcu acuggucuag auguaaugcu accacuaaca      120

cacuuaggga gagccguuuu ucaccuaaau caaagaagag gauuuaaaag uaauagaaaa      180

acggauagag gagauaauga aucaggaaaa auaaaagaug cgacaaaaag guuaacuugg      240

gcaaugcaag aagcuggggc cagaacauau ggagaauuuc uucacaugag aagggcacgg      300

gcaagugauc caaggcaggu accaagcguu agaacaagau uaaguguugc gagcagggga      360

ggacccgaua guaaagagga agcaggguau gauuuuuauc ccgagcgcgc gcacuuagaa      420

gacgaauuuc acgcacuaug gagugcccaa gcugccuucc acccugaucu aacgugugaa      480

uuacgcgaug uaguguuuga gaaaauauuu uaucaaagac cauuaaaaga accaaaggua      540

ggcuuaugcu uauuuacagc ugaagaaagg uuaccuaaag cccauccguu gacugguaga      600

agaguauuau acgagacagu aaaccaauua agaguuacug cagacggaag gacaacaaga      660

ccccuaacau uagaagaaag agacaugauu guauacgcac uugacaauaa aaagccugua      720

aagucauuga gcagcaugca ucuaaaauua guugcucuug guaagcuagu gaaacuaaaa      780

gacggugaac gauuuacauu agagucaggg guaagagaug cgauagcaug ugauccuaua      840

cgagcaacaa ugggucaucc agauggauuc ggaccagcuu ggaguaggcu ggacuggcaa      900

gcucaauggg cacuaauaga aaaauuacgu caaguucaaa gugaugauga uuuugcuucu      960

uuaguagccu gguuaggcgc aacacauaac uugucagaag aucaugcaaa aagaguagca     1020

aauuuacacc uacccgaagg uuauggaagg uugggcuuga ccgcaaccga agcaauucua     1080

uguaaucuaa aagcugaggu auguacguau ucacaagcag uggaaagaug gggcaaacac     1140

cauucaaacc aaaggacagg agaguguuua gaagcucugc cauauuaugg agagguauua     1200

gauagacaug ucauaccugg aacauauaac gaagacgacg augacguuac acgauaugga     1260

agaauaacga auccaacagu ucauaucggu cuuaaccaac uaagacgauu aguuaauaga     1320

auaauugcaa cuuauggcaa accagaucaa auagucgucg aacucgcaag agaauuaaaa     1380

caaucagaag cccagaaaag cgaggcccua aagaaaauaa gagauacaac cgcagaugcg     1440

auaagaagag gacgacaacu agaagagaua ggucagaaaa auacaggugc caacagacua     1500

uuguuaaggu uaugggaaga uuuagggccc gcuguagguc cuagaugcug ccccuauaca     1560

ggaaaaccaa uaucaguuac gauguuauuu gauggagcau gugauguuga ucacauuuua     1620

ccauauucac gcacacuaga cgacuccuuc gcuaaccgua caauauguuu aagagaagcu     1680

aacagacaaa aaggcgauag aaccccaugg gaggcguggg guggaucgga ucaaugggau     1740

gcaauugaag caaacuuaaa agaucuaccg gaaaauaaac gaaaaagauu cgcaccugau     1800

gcuauggaaa gauuugaagg agaaagagac uuuuuagaua gagcucuagu ggauacgcaa     1860

uaccucgcaa gaauaagcag ggcguaucuc gacacacuuu ucacagaagg cggucaugua     1920

uggguaguuc cuggaagaau gacagaaaug cuucgccgac auuggggucu gaacgcauua     1980

cuaagcgaua aagauagagg agccggaaaa guuaaaaaca gaacagauca ucgacaccau     2040

gcaauagaug cagcaguaau ugcagcgaca gaucacaguu uaguaaacag aaucucaaaa     2100

gcagcaggac aaggugaguc agcagggcaa uccgcagaau uaauagcacg ugacacaccc     2160

gaaccuuggg aaggauuuag aaaugacaua agugcacaaa uugcgcguau ugugguauca     2220

caucgagcag aucauggaag aauagaugua gaaggaagaa agcauggaaa agauaguacc     2280

gcaggaagac uacacaacga uacagccuau ggaauaguag augaucauac gguaguaagu     2340

agaacgccac ucuuaucacu uaaacccgga gauauagaag uaaccaccaa agguaaaaac     2400

auuagagaua cacaacuaca aaaagcguua aguguagcua caacaggaaa agauggaaaa     2460

ggauuugaag aagcguuaag aaacuuugca gaaaaagaag gaccuuauca aggcauaaga     2520

agaguaagau uaauagaaac auuacaaagu agugcgagag uagagguagg aucagaugaa     2580

gauggacaac cuuuaaaagc auacaagggu gauaguaacc acuguuacga ucuguggaag     2640

cuaccugaug guaaaguggu accccaugua gucagcacuu augaagcgca cgccggcaca     2700

gacaaaagac cucauccagc agcaaaaaga auauuaagag uauuuaagaa agauauggug     2760

gcuauugaac aagaugauca aacagcaauc uuuuauguac aaaaauuaga uagagcaaau     2820

ggucucuuuc uggcaccaca uuuagaagca aaugcugaug cacgacauag aaaucccaau     2880

gaugccuuua aauuucucca aaugggagcg gggagcgucg uaagaaauaa acugaggcga     2940

guauacguag augaaauagg gagaauaaaa gauccagguc caccaaaaca uuga           2994


<210>  4901
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4901
augaaaauau uaggauucga cauaggaauu aauaguauug gaugggcauu uguugaaaac       60

gaugagcuaa aagauugcgg ggucagaauc uuuacagaag cugagaaucc aucaaauaaa      120

gaaagcuugg cacuuccaag aaggaacgca agaucaagua gaagacguuu aaaaagacga      180

aaagccagau uaguugcaau caagcgaauu cuugccaaag aacuaaaacu aaauuuuaaa      240

gauuaugucg cagcagacgg agagcugcca aaagcauacg aagguaaacu uacuucuauc      300

uacgaacuac gcuacaaagc auuaauacaa caguuagaaa caaaagaucu agccagagug      360

auccuccaua ucgcgaagca cagaggcuau augaacaaga augaaaaaaa gaguaacgac      420

accaaaaaag guaaaauacu cagcgcauua aaaaacaacg cucuaaagcu cgaaaauuac      480

caaagcgugg gugaguauuu cuauaaagaa uucuuucaaa aauauaaaga aaacacaaaa      540

aauuucauua aaauaaggaa uacaaaagac aacuacaaca acuguguacu gucauccgau      600

cuagaaaaag aauuaaaacu aauauuagaa aaacaaaaag aauuuggaua caauuauucc      660

gaagacuuca uuaacgaaau auuaaaggug gcauucuucc aaagaccacu aaaagacuuc      720

agucacuuag ugggagcaug cacauucuuc gaggaagaaa aaagagcaug caaaaauuca      780

uacagcgcgu gggaauucgu agcauuaaca aaaaucauaa acgaaaucaa aucauuagaa      840

aaaauaucag gagaaauagu accaacacaa accauaaaug aaauacucaa cuuaauauua      900

gacaaaggua gcauaaccua caaaaaauuc agaucaugua uaaaccuaca ugaaucaaua      960

uccuuuaaau cccuuaaaua ugauaaagaa aacgcagaaa acgcaaaacu aauagacuuc     1020

agaaaacuag uagaauucaa aaaagcacua ggaguacacu cauuaucaag acaggaacug     1080

gaucaaaucu caacacauau cacacuaauu aaagacaaug uaaaacuaaa aaccguucua     1140

gaaaaauaua auuuauccaa ugaacaaauc aauaaucucc ucgaaauaga auucaaugac     1200

uauauaaauu uaucuuuuaa agcccuuggc augauccuac ccuuaaugcg ugaaggaaaa     1260

agguacuaug aagcaugcga aaucacaaac cuaaaaccaa aaaccgucga ugaaaaagaa     1320

gauuuccuuc cagcuuucug cgauucaauc uucgcccacg aacuaaguaa uccugucgua     1380

aaucgcgcaa ucuccgaaua uagaaaaguc uuaaaugcac uccuuaaaaa auacggaaag     1440

guacacaaaa uacaucuaga acuagcacgc gacguuggau uaagcaaaaa ggcaagagaa     1500

aagauagaag aucaacaaaa aaacaacaaa gcaauaaacg acugggcacu aaaagaaugc     1560

gaaaacaucg gaauuaaagc aagcgcuaag aacauacuca aacuaaaauu auggaaggaa     1620

cagaaagaaa uaugcauaua uucuggaaac aaaaucucaa uagaacaucu aaaagaugaa     1680

aaagcccucg aaguugacca caucuaucca uacucuagau cuuuugacga cucauucaua     1740

aauaagguac ucgucuuuac aaaagaaaac caagaaaagc uaaacaaaac accauuugaa     1800

gcauucggaa aaaauaucga aaaauggagu aaaauccaaa cuuuggcaca aaaucucccc     1860

uauaaaaaaa aaaauaaaau cuuagacgaa aauuucaaag auaaacaaca acaagacuuc     1920

auaucuagaa acuuaaacga cacaagauau auaacaaccc uaauagcaaa auacacaaag     1980

gaauaucuca auuucuugcc cuuaagcgaa aacgaaaaca ccaaccuaaa gucaggagaa     2040

aaaggcagua aaauccacau acaaacaaua ucugguaugc uaaccucagu cuuaagacac     2100

acauggggau ucgacaaaaa ggacagaaau aaucauuugc accacgcauu agaugccaua     2160

aucguagcau acucuacuuc aucaaucaua aaagcauucu ccgacuuuaa aaaaaaucaa     2220

gaauuauuaa aagcacgauu cuacgcaaaa gaacuaacau cagacaacua caagcaucaa     2280

gucaaauuuu uugaaccauu caaaggguuu agagaaaaaa uucuaaguaa aauugacgaa     2340

auauucguuu caacaccccc acguaaacga gcaagaggcg cuuuacacga agaaacauuc     2400

uauucggaag acaaaaugau caaaaaguau aauucaaaag aaggauuaca aauagcuuua     2460

ucaugcgggc gagucagaaa aaucggaaca aaauauguag aaaacgacac caugguucga     2520

guagacauau ucaaaaaaca aaauaaauuc uacgcgaucc caauauacgu gauggacuuc     2580

gcacuaggaa uccuacccaa caaaauagua auaacaggaa aagacaaaaa caacaaucca     2640

aagcaauggc aaacaauaga cgaaucuuac gaauuuuguu uuucauuaua caaaaacgac     2700

cuaauacugc uccaaaagaa aaacaugcaa gaaccagaau ucgcauauua uaacaguuuc     2760

gacauagaua gaucaaggau aaaaauaaaa aaacacgaua acaaauuuga aaacuuaaca     2820

agcaaccaaa aacuacuauu uacaaacaaa gacaaaggca aaaaccgaac uggcauucaa     2880

aaccucaaga uauucgaaaa auacauagua acaccacuag gagauaaaau uaaggcagac     2940

uuccagcccc gcgaaaauau aucauuaaag acuucuaaga agcauggccu auga           2994


<210>  4902
<211>  2994
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4902
auggguaauu acacauuagg auuggauauu gguagcaauu caauuggaug ggcauuaguu       60

gaagaugcgg ucgaggcacc acgcauagua gauaugggag ugagaguauu uccggaaggc      120

guggaccgcg acacgaaagg auuggaaaaa ucgaaaaacg caacgaggag agaagcacgu      180

ggcgcgagac ggacacacca gagaagaagg caaagaaaac aaagacuugu gagagcacua      240

agacaaguag gucuacugcc augugacaag gccgaaguaa gaagccuguu ucagaccgac      300

ccuuacgaaa uaagaaaaag agguuuagac cauagauuag aacucuauga aauaggaagg      360

gcauuuuauc accuaucuca aagaagagga uucaaaagua auagaaaauc aggcgcucuc      420

aaagaagacg gaacggucaa gaaagaagcu ggagaguugc aaagaaaaau agaugaggca      480

ggauguagaa cuaucggaga auauuuugca ggccuagacc cagaacagca aaggauccgc      540

ggcagauaua cuuucagaag uauguaccaa aaagaauuug aucuacuaug ggcuaaacaa      600

guagaauuuu auccgaaagu auuaacagag gauuuaagau caaaaguacg cgaugaaaua      660

aucuucuuuc aaagaccacu aaaaagcgcc aaagacuuaa uaggagauug ugaacuagaa      720

cccacagaaa aacgaugucc ucgagcagau ugguaugcaa ggagauuuag auuacuacaa      780

gauauuaaua auuuaauaau acaaaauccg gacgguacag aacagaaacu aaguccagac      840

caacgagcag cacugcuugg cgaacuagga caaagagaaa aacuaaccuu cgauaacaua      900

agagcaaagc uaggauuauu agaaacgcaa aaauuuaaug ccgaauauag aauugguaga      960

aaagguaaga aaguagaagc aauaaaaggc gacguauuug ucuggacuau gaggggaaaa     1020

ucgguuuuug gaccaaaagc cugggaugca auggcugaug aaagaaaagu uagcuuaaau     1080

gaauuguuug uagaguuaga ggacgaugau uuagcuagaa aauuagagac cgaauauggc     1140

uuaacagaua aacaaguaca agcagcaaug aaggcuucuc ucccacaagg auacaugagu     1200

uucucaagaa aagcaguuuu aaaacuccua ccauuuaugg aggcaggagc auuaacucau     1260

gaagccauag aucaagcagg auauagaaaa gaagaaccaa cacaaacaga ugcaauagca     1320

agaguaaguu uuccuccaga ucugagaaau cccauagugc aaaaagcauu auucgaggug     1380

cgaaaagugg ucaacgcccu aguacgagag uauggaaaac cgacaagaau caaaauagaa     1440

auggcacgag acguucaagg uagcaaaagg caaagagaag aauugcauug gcgaaaugaa     1500

gauaacagaa agaaaaacga agaaguacgu gacagauuaa gaacugacau acacauagac     1560

gauccuagca gagacgaugu aauuaaauau aaacuauggc aggaaagcgg uaaaaugugc     1620

ccauauacag gaagacaaau augucaagaa gcguuguuug gacccaaucc agaguuccaa     1680

gucgaacaua uccuaccgua ugacagaucu cuagaugauu ccuacaugaa uuuaacauua     1740

ugcgaaguuc augaaaacau acauggcaaa agaaaucaaa caccauuuga agcauacgga     1800

aguggagcag aacgauauga acaaauaaag caaagaauua aagucuuacc uuggccgaaa     1860

agacagaaau ucuugcaaca agaaaucaau cuagauagcc auauaagcag ggaacuuaau     1920

gauacaagau acauaugcag agaaacagua gcauaucuaa agcaacuagg aguccaugua     1980

aggggaacaa gaggaaaaau aacagcagaa uuaagacauc aauggggauu agauggaauu     2040

uucagugaau uagguguacg aagagaugau gaccaccgac gacaugcagu agacgcauug     2100

gucgucgcug ucaccgauaa ugaacauuua cgucgauuag cggugaguaa auacguauua     2160

gcaggcccaa gguuugaaac accguggagu gccuucagag augaagcaag agguaacgua     2220

aagagaauga auguaucgca ucgaguuugu agaaaaguua guggugcgcu acaugaagag     2280

acaaacuacg gaccgacggg cguacgagau caagcaggac aagauauaua cgcguauaga     2340

aagaaacuag aagacuuaac aaccccaaug guagagaaaa uagucgaucc ugucguacga     2400

gacauaguua gacaaagauu auuaaugaag ggaauagacc cugcagcugc agacagaaaa     2460

auaccaaaag auguauggaa agaaccacua uacaugcgua gaacaaaauc agauaaaaug     2520

auacaaauaa gaagaguuag aauacacgau guuaaaaacu cuauuauugu auugacagac     2580

gaaucaggcc gaccauauag agcaguagaa ccaggaggaa aucaccaugu cgagguauuc     2640

gaguacguac acgggacaaa aacugguacc cgagaagccg aaguaguaac aauguuugaa     2700

gcuguccgaa gaucaagggc aagagcacca guggugagaa gagaucacgg uccagguaca     2760

agguucguau gcucuuuagc aauaaaugac augguuauga ugcccgacaa aaguggaaaa     2820

auggaucucu uuagaauaca aaagauguca gccagcaaac aaguauacuu uagacaucau     2880

acagcggcuc gaaucgacag cgaaagaaca uuaauaagaa aacaagccca ucuauucaau     2940

ggauauaaag ugucuguuga cccuacugga gcaauuugua gagcaaauga uuga           2994


<210>  4903
<211>  3000
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4903
augaaaauac ugggauucga uauaggaaua aacuccauug gaugggcauu uguagaagac       60

aaucaauuac aagauugugg aguacgcuua uuuacaaaag cagaagaucc aaaaaccaaa      120

gagucacugg cacuaccuag aaggaacgca agaucaaaca gaagaagauu aggccgcaga      180

cgaucacgac uaauugcauu aaaacauaua auuagcaaag ggcuaaaacu aaacuaccaa      240

gacuacauag cuaacgacgg agaacuacca aaagcuuaug aaggacgcuu aauaucucca      300

uacgaacucc guuauaaagc ucuaaaugaa aaaaucgaac caaaagaccu agcacgagua      360

auacuacaca uagcaaaaca ccgaggcuac augaacaaaa acgaaaaaaa gucaucagac      420

aacgaaaaag guaaaauacu aagcgcauua aaaacaaacg cacuaaaacu agaaaaauac      480

caauccguag gagaauacuu uuacaaggag uucuuucaga aauaccgaga aaacacgaag      540

gacuucauaa auauaagaaa uaaagaagga agcuaugaaa auuguguauu agcuagugac      600

uuagagaaag aauuaaaauu gaucuuggaa aaacagaaag aauggggaua cuccuauaau      660

gauaacuuca uaaaagaaau uuuaaaagua gcauuuuucc aaagaccucu caaagacuuu      720

ucuuaucucg uaggagcaug uacauucuuc gaagacgaaa aaagagcuug caaaaauagu      780

uacucugcau gggaauucgu agcacuaacc aagauaauca augaacucaa aucauuagaa      840

aaagaaagcg gagaacuggu gucaucacaa aucauaaacg aaauacuaaa ucauauauua      900

gauaaaggca guaucacuua caaaaaauuc agagaauaua uaaaacuaca ugaaucaaug      960

aaauucaaaa gucucaaaua cgauaaggau aaugucgaaa gcacgaaacu aauagaauuu     1020

cgaaaacuag uagaguuuaa aaaagcacua ggugagcacu cccucucaag agaagaacuu     1080

gaccaaauug ccacauacau aacacucaua aaagauaaug aaaaauuaaa aauaacauua     1140

gagaaauacu cacuaaacaa ugaacaaaua aaaaaccucc uagaaauaga uuuuaacgau     1200

cauaucaacc uaagcuucaa agcccucaau cugauacuac cauuaaugaa agaagggaaa     1260

agguacgacg aagcaugcaa acugcuaaau cuaaaaacaa aauccaauaa ucaaaaauuu     1320

gauuuucuac ccgcauuuug ugauucaaua uuugcccaag aauuaacuaa cccaauagug     1380

aacagagcaa uaagcgaaua ccgaaaagua cuaaaugcac uacuaaaaaa auauggcaaa     1440

augcauaaaa uucacauaga acuagcuaga gauauaggau uaucaaaaaa gcucagagca     1500

aaaaucgaaa aagaacaaaa agaaaacuac gagaacaaca uaugggcccu aaaugaaugc     1560

gaaaaauucg gucuaaaagc aaacgcaaaa aacaucuuaa aauuaaaacu uuggaaagaa     1620

caaaaagaau uuuguauaua uuccggaaaa aaaauuagca ucgagcaccu ucgagacgaa     1680

aaagcguuag aaguugacca uauuuaccca uacucaagaa gcuucgacga cucauuccua     1740

aacaaaguuu uagucuucac aaaagaaaac caagaaaaau uaaacaaaac acccuuugag     1800

gcauucgggg cuaaugaaga aagauggagu aaaauacagg cguuagcaca aaacuuacca     1860

uacaaaaaaa aaaauaaaau acuggaugaa gcauuuaaag gaaaacagca gcaggauuuu     1920

aucucgcgaa accucaacga caccagauau auaucgacac uuauaguuaa guacacaaaa     1980

gaauaccuag acuuccuacc cuuagaugaa aaagaagaug ucaaccucaa aucaggagaa     2040

aaaggaucaa aaauucaugu acaaacaaua aauggcaugc uuacaucagu ucugagacac     2100

acauggggau uugcacaaaa ggauagaaac aaccaccugc aucaugcccu agaugccacc     2160

auaguagccu acucaacaaa cgcaauaaua aaagcauucu cagacuuuaa aaaagaacaa     2220

gagcuacuca aagcaaaacu uuaugcuaaa gaacugacaa gcgauucaua caagcaccag     2280

gcaaaauuuu uugaaccauu cgaggguuuu agagaacaaa uacuuaacca aauaaacaaa     2340

cuauuugucu caaaaccacc aagaaaaaga gcacggggag cacuucauaa agaaacguuu     2400

uacucaaaag acgaaaugau aaaaaaauac aauucccaag aaggaguaga aauagccuua     2460

aacugcggaa aaauaagaaa aauaggaaca aaauacguag aaaacgauac aaugguccga     2520

aucgauauuu ucaaaaaaca aaauaaauuc uacgccauac cuauauacac cauggacuuu     2580

gcauugggcg uauuaccaaa caagauagua auaauaggaa aagacaaaaa aggaaauccu     2640

aaacaauggc aggaaauaga ugaaucauau gaauuuugcu uuagcuugca caaagacgac     2700

uuaguacuua uucaaaaaaa agauaugcaa gaaccagaau ucgcuuacua caacggcuuc     2760

gacauuagua auagcucaau cugcguagaa aaacaugaca acaaauucga aaaucuaaca     2820

gacaaucaaa gguuauuauu uacuaacgca gaaggaaaug ucaaagcaaa aaaaauagga     2880

auccaaggac uacgaauauu cgagaaauac aucauaacac cccuugguga aaagauaaaa     2940

gcagacuuua aaccaagaga ggacauagca uuaaaaacau caaaaaaaca cggauuauga     3000


<210>  4904
<211>  3003
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4904
augaagauau uaggauucga uauagguaua aauucaauag gaugggcauu cguagaaaac       60

gacgaacuca aagacugcgg aguaagaaua uucacgaagg ccgaaaaucc aaaaaacaaa      120

gaaagccuag cacuaccacg cagaaaugca cgcucuucca gaagacgacu caaaagaagg      180

aaggcaagac uaaucgcuau aaaaagaauc uuagcaaaag aacucaaauu aaacuucaag      240

gauuacgucg cagcagaugg agaacuacca aaagccuacg aaggcucuuu agcaucugua      300

uacgaacuca gauauaaagc acucacgcag aaauuagaaa caaaagaccu agcaagggua      360

auacuacaua uagcaaagca uagaggguac augaacaaaa acgaaaaaaa aucaaaugac      420

gcaaaaaaag gaaaaauacu aucugcccua aaaaauaaug cccuaaaauu agaaaauuau      480

caaagcgucg gcgaauauuu uuauaaagag uucuuucaga aguacaaaga aaauacaaaa      540

aacuuuauaa aaauaagaaa cacaaaggac aacuauaaca acuguguccu cucuucugac      600

cuagaaaaag aacucaaauu caucuuagaa aaacaaaaag aauucggcua uaaguauucu      660

gaaggcuuca uaaacgaaau acugaaagua gcguuuuuuc aacgaccgcu aaaagacuuu      720

ucucaccuug uuggagccug caccuucuuu gaagaggaaa aucgagcaug caaaaacucc      780

uauucagcau gggaauucgu agcauugaca aaaauaauaa acgaaaucaa aucgcuagaa      840

aaaauaucag gagaaauagu aucuacacaa acaauaaaug aagugcuaaa ccuaauauua      900

gacaaaggcu ccauaacaua uaaaaaguuc aggagcugca ucaaucucca cgaaucaaua      960

agcuuuaaau cguuaaaaua cgacaaagaa aacgccgaaa augcaaaacu aauagacuuu     1020

cgcaaacucg uggaauucaa gaaagcauua ggcgugcauu ccuuaucaag gcaagaauua     1080

gaucaaauau ccacacacgu aacauuaaua aaagacaacg uaaaacuaaa aacaguacua     1140

gaaaaauaca accuaucaaa cgaacaaauc aacaaucuau uagaaauaga auuuaaugau     1200

uauaucaacc uuucauuuaa agccuuaggg augauauuac cccucaugcg agaagguaag     1260

agauaugaug aagcaugcga aauuacaaau cucaaaccaa aaaccguaga cgaaaaaaaa     1320

gauuuucuac cagcauucug cgacaguauc uuugcacacg aacuuucaaa cccaguagua     1380

aacagagcca uaagugaaua ccgaaaagua cuaaacgccu uguuaaaaaa auacgauaaa     1440

guccauaaaa uccauuugga auuagcaagg gacguaggcc uuagcaaaaa agcacgggaa     1500

aaaaucgaaa aggaacaaaa agagaaccaa gcaguaaacg caugggcucu aaaagaaugu     1560

gaaaacaucg gucucaaagc cucggccaaa aauauacuaa agcuuaagcu auggaaagag     1620

caaaaagaaa uuugcaucua cucaggaaau aaaauaagca uagaacauuu aaaagacgaa     1680

aaagcgcuag aaguagacca uauauaucca uauagucgua guuucgacga caguuuuaug     1740

aauaaaguau uaguauucac uaaagaaaau caagaaaaac uaaauaaaac accuuuugaa     1800

gccuuuggaa aaaacauuga aaaaugguca aaaauacaaa cacuagcuca aaauuuacca     1860

uacaaaaaaa aaaacaaaau auuggacgaa aacuuuaagg auaaacaaca agaagacuuu     1920

auauccagga accuaaacga cacaagauac auugcuacgc uaauaaccaa auauacaaaa     1980

gaauaucuaa auuucuuacc ucucaaugaa aaugaaaaua ccaauuuaaa aucgggagaa     2040

aaagguagca agauacacgu ccaaacaguc aaugguaugc ucaccucagu acuaagacau     2100

acauggggau ucgacaaaaa agaccguaac aaccaucuac accacgcccu cgacgcaauc     2160

auaguagcau acagcacaaa cucaauaaua aaagccuucu cagacuucaa aaaaaaccaa     2220

gaacuacuaa aagcacgguu uuaugcaaaa gaacuaacca gcgauaauua caagcaucaa     2280

guuaaauucu ucgaacccuu uaaagguuuu agagaaaaaa uccucucaaa aauagaugaa     2340

aucuucguau caaaaccacc caggaaacga gcuagaggcg cguuacacaa agaaaccuuu     2400

uacucagaag acgagaugau aaaaaaauac aacucgaaag aaggucugca aauugcccuu     2460

ucaugcggcc gaguaaggaa aauagguaca aaauauguug aaaacgacac aauggucaga     2520

guagauauau uuaaaaagca aaacaaauuu uaugcaauac caauauaugc gauggauuuc     2580

gcacuaggca uauuaccaaa caaaauugua guaguaggca aagauaaaaa uaauaacccu     2640

aaacaauggc aaacuauaga ugaaaguuau gaauucuguu ucucccugua caaaaacgau     2700

uuaauaaugc uucaaaaaaa ggacaugcaa gaagcagaau ucgcuuauua uaaugguuuc     2760

gacaucucaa caucuagcau cuguguugaa aaacaugacu caaaauuuga aaaucucaca     2820

aguaaucaaa aacuacuauu uuccaaugcu aaagaaggaa gcguaaaagu agaaaaccuc     2880

ggaauacaaa accuaaaaau auucgaaaag uacauaauaa caccauuagg cgacaagauc     2940

aaagcagauu uccaaccacg cgaaaacaua agucucaaaa caagcaaaaa aaacgguuua     3000

uga                                                                   3003


<210>  4905
<211>  3003
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4905
auggcaaggg cucagagacg uuuaauaucc agaagagcca acagaaugaa uaaacugaga       60

agauuauuaa aacaagaaaa uguuuuacaa gcuaacgauu augacgaaaa aggccuaauu      120

auaggacuac ccaaucaagc augggaacuu cgcgcacaag gccuagauag aaaauuagaa      180

ccaaaagaau gggccgcugu guuaauaaac cucguaaaac auagagggua uuuaagucaa      240

cguaaaaaug aaucacaaac agcagacaaa gagcuaggag cccuauuaag uggaaugaau      300

cauaaucauc aacugcuaca aucacaaagu aaucaauaca gaacuccagc agaauuagca      360

auaaaacaau uugcgacaca agauggacau auuagaaacc agagaggagc auacacacac      420

acuuuuaaua gauuagaccu acaaaacgag uuaaaucagc uauucgacgc acaaacaaga      480

cuuggcaauc cacacguaag uggaucccuc aaaaccgcga uaaaugacuu acuaaugaau      540

caaaaagcag cauuaucugg cgacgcgauc cuugaaaugc uaggacgaug uacauucgaa      600

ccaucagaau auaaagccgc aaaaaauacc uacagugcgg aaagauuugu auggcucggc      660

aaacuuacaa acuuaagaau acaagauaau ggauuagaaa gcgcccuuuc aagagaacaa      720

agggaacuac uacuuaauga accauauaac aaaacaaaac uaacauacca acagguucga      780

aaacuacuac ggcuaccaga aacagccuuu uucaaagguc uaagauaugg uaaagacaac      840

gcagaaucag caacauucau ggaaaugaaa ucauaccacg caauaagaaa aucucuagaa      900

aacaaaaguc ucaaaaguga augggaaaaa cuaaaaacuc agccaaaacu ccuugaucua      960

auuggcacug cauucaguau auauaaaaca gaugaugaaa uugccggaug ucuaaaaaau     1020

cagcucccag aaaauguuau aauugcgcua caagaacaau uaaauuucag caacuuuaug     1080

gaauuguccu uaaaagcacu agaaaaauug cuaccacuca uggaacaagg uuugcgauac     1140

gaugaagcau gcacgcaaau uuacgguaau agacacgaaa aaauagccga uaaaacagag     1200

aaauuccucc caaacauacc ggcugacaaa uuacgcaacc cugucguuuu aagaacauua     1260

acacaagcaa gaaaaguuau aaacgccauc auucgaagau augguagccc agccagggua     1320

cacauagaaa cagguagaga aguaggaaaa uccuuuucag acagagacaa acucaaaaaa     1380

cagcaagaag aaaaucaaga acaaagaaga cgcgcaauag acgaauggaa acaauuuuuu     1440

ccaaacgcag cucacgaacc aaaagcaguc gauauucuaa aauuaagacu auaucaacua     1500

caacagggua aaugucuuua uagcgggcuu ccaaucgacg ucagaaggcu accagaaaaa     1560

ggauacguag aaauagauca cgcacuacca uuuuccagaa caugggauga cucauucaau     1620

aacaaaaucc uaguacuagc cucagaaaac caaaauaaag gcaaucaaac accuuacgaa     1680

uaccucagag gagcagacaa cuccgaaagc uggcgacuuu uuguugaaaa uguacaaaac     1740

ugccaauuua guccaaauaa aaagcaaaaa auaauggcaa aacagcuaga ugaaaaagga     1800

uuuuuagaaa ggaaccucaa cgacacgcga uaugucagcc gcuuuuuaug ccaauucaua     1860

agugaacauc ugcauuuaac agguaaagga aagaaacgag uuuuugccuc uaauggccaa     1920

auaacugcac uauuaagagg aagaugggga uuaucaaaaa aucgugaaga aaaugauagg     1980

caucaugcag uagacgcugu cguaguggca uguagcacgg uugcaaugca acaaaaaaua     2040

acacaauucg ucagauauca aaaaggaaau gucuuuacug gagaacgaau agacaaagaa     2100

acaggagaag uaauauccau ccauuuccca acucccuggg aacacuucag agaagaaguc     2160

augauccgag uauucgacga uaauccacua cgcacauuac cagaaaaguu acccaacagg     2220

cccgaagcuc uacacgaaua cguuaugccu cuauuuguaa guagaaugcc aacaaggaaa     2280

augacaggac aaggacacaa ggaaacaaua aaaucugcca agcgccucga ugaaaaaaua     2340

agcguauuaa gaguaccacu uacccaacuc aaauugaaug acuuggagaa caugguuaac     2400

agagaaagag aaccagcacu auaugaagcu cuaaaagcac gucuagaaca auuuaaaaac     2460

gauccugcaa aagcuuuugc ugaaccauuu uucaaacaag gcggacaaca agucaaagca     2520

guaagagucg aaauaguaca aaaaaguggc augcuauuac guaaaauaaa uggggucgcc     2580

gauaauggau cgaugguaag aguagaugua uucgaaaaaa guggaaaauu uuaccuagua     2640

cccaucuacg cguggcaggu ugcagaaggu auacucccaa acuaugcagu cgucgcacac     2700

aaagaugaaa gcgacuggau auuaauggau gaaaguuauc acuuuaaauu ugcauuauau     2760

aaaaaugacc uacuagaagu ccaaacaaaa aaagguggua uaaugggaua uuucgcaagc     2820

uuagaucgag caacgggcgc caucacaaua agagaacacg aucgugauaa aaaaaaaugc     2880

aaagauggcg agcaucgauc aauaggagug aaaauggcau cauccuuuaa aaaacuucag     2940

gucgaugaac uuggcaaaaa uauacacgaa acaagaaguc gaaaaagacc uaauauagga     3000

uga                                                                   3003


<210>  4906
<211>  3012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4906
augcgaauuc uagguuuuga uauuggaaua aacucuaucg gaugggccuu cguagaaaac       60

gacgaacuca aagauugcgg agugagaaua uucacaaaag cugaaaaccc aaaaaacaaa      120

gaaucacuag cacuccccag aagaaacgca aggucaucac gacgacgauu aaaaagacga      180

aaagcucgac ucauagcaau caaaagaaua cuagcaaaag aacuaaaacu aaacuacaaa      240

gacuacgugg cugcugaugg agaauuaccc aaagcauacg agggaucacu agccagcguc      300

uacgaacuuc gcuacaaagc acugacacaa aaucuagaaa caaaagaucu cgcacgagua      360

auacuccaca ucgcaaaaca uagaggauac augaauaaaa acgaaaaaaa gaguaaugac      420

gccaaaaaag gaaaaauuuu aucagcacua aaaaacaacg cacuaaaacu cgaaaacuau      480

caaagcgucg gagaguacuu uuauaaagaa uucuuccaaa aguacaaaaa aaauacaaaa      540

aauuucauua aaaucagaaa cacuaaagau aacuacaaca auuguguauu aucuagcgau      600

uuagagaaag aacuaaaacu uauccuugaa aaacaaaaag aauuuggaua caauuacucc      660

gaagacuuua ucaacgaaau acuaaaaguc gcauucuucc aacgaccccu caaggacuuu      720

agccaccuag ucggagcaug cacauuuuuu gaagaagaaa aaagagccug uaaaaacucu      780

uacucagccu gggaauuugu agcccuaaca aaaauaauaa augaaauaaa aucuuuagaa      840

aaaaucagcg gagaaaucgu uccaacacaa acaauaaacg aaguucucaa cuuaauacuc      900

gacaaagguu cgauaaccua caaaaaauuc agauccugca ucaacuuaca ugaaucaauc      960

ucauucaaau cacuaaaaua cgauaaagaa aacgcagaaa augcaaaacu aauugauuuu     1020

cggaaacuag uagaauucaa gaaagcacua gguguccauu cucuaucuag acaggaacua     1080

gaucaaauau caacacauau aacacuaaua aaggacaaug uaaaacuaaa aacaguucuu     1140

gaaaaauaca aucuuucaaa ugaacaaaua aauaaccuac uagaaauaga auucaacgac     1200

uauauaaacc ucucauucaa agcccuagga augauccuac cccucaugag agaaggcaaa     1260

cgauacgaug aagcaugcga aaucgcaaac cuuaaaccaa agacagugga cgaaaaaaaa     1320

gauuuccucc ccgcauucug cgauaguauc uucgcucaug aacucucaaa ucccguuguc     1380

aaccgagcaa uaagcgaaua cagaaaaguu uuaaacgcuc uccuuaaaaa auacggcaaa     1440

guucacaaaa uccaccuaga acuagcacgu gauguaggac uuucaaaaaa agcacgagaa     1500

aaaaucgaaa aagaacaaaa agaaaaucaa gcaguaaacg caugggcgcu uaaagaaugc     1560

gaaaacauag gauuaaaagc aucagcgaaa aacauacuaa agcuaaagcu auggaaagaa     1620

caaaaagaaa uauguaucua uagcggcaac aaaauaagua ucgaacacuu aaaagacgag     1680

aaagcucuag aaguugauca uauauaucca uacucuagaa guuucgauga caguuucauu     1740

aauaaaguac uaguauuuac uaaagaaaac caagagaaac uaaauaaaac ccccuucgaa     1800

gcuuuuggca aaaacaucga aaaauggucc aaaauucaga cccuugcaca aaacuuaccc     1860

uacaaaaaaa aaaacaaaau ccuagacgag aacuuuaaag acaaacaaca agaagacuuu     1920

auauccagaa accucaauga cacuagauau aucgcaaccc ugauagcaaa auauacaaag     1980

gaauaucuaa acuuuuuacu ccuaucagaa aaugaaaaug caaaccuuaa aagcggagag     2040

aaaggaagua aaauacacgu acaaacaaua agcgggaugu uaacaucagu acuccgacac     2100

acauggggau ucgauaaaaa agauagaaau aaccaccuac accacgcucu agacgcuaua     2160

aucguugcuu acagcacuaa cagcaucaua aaagcauucu cagauuucag aaaaaaccaa     2220

gaguuacuaa aagcccgauu uuaugccaaa gagcuaacca gcgacaauua caaacaucag     2280

guaaaauucu ucgaaccauu caaaagcuuu cgagaaaaaa uacucuccaa aaucgaugaa     2340

auuuucguaa gcaaaccucc ccguaaaaga gcaaggagag cccuccauaa agacacauuc     2400

cauucugaaa acaaaauaau ugacaaaugu ucuuauaacu caaaagaagg acuacaaaua     2460

gcauuaaguu gcggucgagu acgcaaaauu ggcacaaaau augucgaaaa cgauacaauc     2520

guccgaguug acauauucaa aaaacaaaac aaauucuacg cuaucccaau auaugccaug     2580

gacuucgcau uagguauacu accaaacaag aucguaauua ccggaaaaga caaaaacaau     2640

aacccaaaac aauggcaaac aauagacgaa ucguacgaau ucugcuuuuc ccuauacaaa     2700

aaugaccuaa uauuacugca aaaaaaaaac augcaagaac cugaauucgc auauuacaau     2760

gacuucagua uaucaacauc aucaauuugc gucgaaaaac acgauaauaa auuugaaaac     2820

cucacaucaa accaaaagcu auuauucucc aaugcaaaag aaggaucugu caaaguagaa     2880

uccuuaggaa uacaaaaucu aaaaguauuc gaaaaguaca uaauaacacc ccucggcgau     2940

aaaauaaagg cagacuucca accuagagaa aacauuagcc uaaaaaccuc aaaaaaguac     3000

ggcuuaagau ga                                                         3012


<210>  4907
<211>  3012
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4907
augauagacg agagcuugac cuuuggcaua gauuuaggaa uagggucuug cgguugggcg       60

guacuucgca ggcccagugc cuuuggaaga aaagggguua uagaaggaau ggguucaugg      120

uguuucgacg uaccagagac aaguaaagaa agaacuccaa caaaucaaau aagacgaucu      180

aauagacuau uacguagagu caucagaaga agaagaaaca gaauggcugc aauaagaaga      240

cugcuccacg cagcaggucu acuaccuuca acugacuccg augcuuuaaa aagaccgggg      300

cacgacccgu gggaacuaag ggcuagaggg cuagauaaac cacucaaacc uguagaauuu      360

gcagucguau ugggacacau agcaaaaagg cgaggauuua agucagcugc uaaaaggaag      420

gcuacaaaua uaucauccga ugacaagaaa auguuaacgg cacuagaagc aacacgugaa      480

aggcuagguc guuaucgcac cguaggugaa auguuugcca gagaccccga uuuugcauca      540

aggaggagaa auagagaagg aaaguaugac cguacaacug caagagauga cuuagagcau      600

gaaguacaug cauuauucgc ugcccaaagg cgacuuggac aaggauucgc aagucccgaa      660

cuagaggaag cguuuacugc aagugcauuu caccaaagac cuaugcaaga uucagaaaga      720

uuaguaggau ucugcccuuu ugagaggacu gagaaaagag cagcaaaacu aacuccaagu      780

uuugaaagau uccgacuauu agcuagguug cuaaaccuga gaauaacaac accagaugga      840

gaaagaccau uaacaguuga ugaaaucgcu cuugucacca gagauuuagg aaaaacagca      900

aaauuaagca uuaagagagu uagaacauua auagggcuag aagauaauca aagauuuacu      960

acgauucgac ccgaagauga agauagggac auaguugcaa ggaccggcgg agcuaugaca     1020

ggcaccgcua cauuaagaaa agcacuagga gaggcauugu ggacagacau gcaagaaaga     1080

ccagaacagu uggaugccau aguacaagug cucagcuucu uugaagcaaa ugaaacaaua     1140

acagaaaagu uaagggaaau aggauuaaca cuugcuguuc ucgauguauu acugacagcc     1200

cuagaugcgg ggguuuuugc uaaguucaaa ggagcugcac auauaucaac aaaagcagca     1260

cgaaacuuau uaccucaccu cgaacaagga aggagauaug augaagcaug uacaauggca     1320

ggauacgacc augccgcauc aagacuaucu caucauggac agauuguugc uaaaacccag     1380

uucaaugcau uagugacaga gauaggagag ucaauagcaa auccuauugc aagaaaagcu     1440

cucaucgaag gguuaaaaca gauaugggcc augaggaacc acugggggcu gccaggaucg     1500

auacauguag aauuagcaag agacguugga aauaguauag agaaaagaag ggaaaucgaa     1560

aagcauauag agaaaaauac agcgcuacga gccagagaaa ggagggaagu acaugacuua     1620

uuagaccuag aagacguuaa cggagauacc uuacuacgau auagauugug gaaagaacaa     1680

ggaggaaaau gucuauauac uggaaaagca auacauauaa gacaaauugc ugcaacagau     1740

aacagcgugc aaguagauca uauauuaccu uggucaagau ucggcgacga cucauuuaau     1800

aauaaaacau uauguuuagc cuccgcaaac caacaaaaga aaagaucaac uccauacgaa     1860

uggcuaagcg gacaaacggg ugaugcuugg aaugcuuucg uucaaagaau cgaaacaaau     1920

aaagaacuaa ggggauuuaa gaaaagaaac uaucuacuua aaaaugccaa agaagcagaa     1980

gaaaaauuua gaagucgaaa ucuaaaugau acacgguacg cagcgcgauu auuugcagaa     2040

gcagugaaau uauuauaugc uuuuggugaa agacaagaaa aaggcgguaa uaggagaguu     2100

uucacuagac caggugcacu aaccgcagca uuaaggcaag cauggggugu agagagucua     2160

aagaaacaag acggaaaacg aauaaacgac gacagacauc acgcauuaga ugcauuaacg     2220

guagcggcgg uagaugaagc agaaauacaa agauuaacua aaucuuuuca ugaaugggaa     2280

caacaagguc ucggaagacc auuaagaagg guagagccac ccugggaaag uuuucgagca     2340

gauguagaag cuacauaucc agagguuuuu gucgcuagac cagaacgaag gagagcuagg     2400

ggagaaggac augcagcaac gauaagacaa guaaaagaga gagaguguac uccaauuguu     2460

uuugaaagga aagcuguauc aucauugaaa gaagccgauu uagagagaau aaaagauggg     2520

gaacgaaaug aagccauugu agaagcaaua agaucaugga uagcaacagg cagaccugca     2580

gacgcaccgc caagaagucc uagaggcgau auaauaacaa agaucaggcu agcaacaacu     2640

auaaaagcug ccguaccugu cagaggagga accgcaggga gaggagaaau gguaagggca     2700

gauguauucu cuaaaccaaa uagacgagga aaagaugaau gguaucuugu accgguauac     2760

ccccaucaaa uaaugaauag aaaagcaugg ccaaaaccgc cgaugcguuc aauuguugca     2820

aauaaagaug aagacgagug gacugaagua ggucccgaac accaauuuag auuuucauua     2880

uaccccagau caaauauaga aaucauucga cccaguggug aagugauaga gggauauuuc     2940

guaggacuac acagaaacac aggagcacuu auucccaccc caguaggacc ugacucauau     3000

gugauagcau ga                                                         3012


<210>  4908
<211>  3024
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4908
augaaaguuc ugggauucga caucggaauc aacagcauag ggugggcauu uguagaaaac       60

aacgaauuaa aagacugcgg aguucgacuc uuuacaaaag cugaaaaccc aaaaacaaaa      120

gaaucauuag cacuaccaag acgaaaugca cgaaguucaa gaguccggcu acgcagacga      180

agaaguaggc ugcucagccu aaaacacaua auaucuaaag aauucggacu acacuacgaa      240

gauuacauug cacucgacgg gaaucuacca aaagcuuaca ccggaaaaau agaaucaaua      300

uacaaauuaa gauacgaagc gcuaagccaa gcaauaagca aagaagaccu agcaaggguu      360

auccuucaua uagcaaaaca cagaggauau augaacaaaa accaaaaaac aucccaagau      420

ucagaaaaag gagccauucu aucagccauc aaagaaaaua guaacaaaau caagcauuau      480

caaacagcag gggaauauuu uuauaaagaa cuauucgaga accuaaggga ugguacuaaa      540

gaguucaaaa auauaagaaa caaagcaaug aaaaaugaaa aaacgggaaa cauggaauca      600

acauacuaua auaccauacu agcaucugau cuugaaaaag aauuaaaacu gauucuagaa      660

aaacaacaag ccuaucacaa uuacagugac acauucauaa aagaaguauu ggaaauagca      720

uuuuaccaac gcccucuaaa agacuucagu caucugguag gcagcuguac auuuuacgaa      780

aaugagaaga gagcaugcaa aaacagcuau ucagcauggg aauucauagc uuuagguaag      840

auaauaaaca cacuagaauc aauuaaaaaa gacacaggag aaauauucag caaugaaaua      900

auaacacaaa uuuuaaaaga aguacuagau aaaggauccc ucaguuacac aaaauuaaga      960

aaaaugauaa aucuagauga gaaaguaaaa uuuaaaggau uaaaauauga caaagaagga     1020

guagaaaaaa cgaaucuaau agaguucaaa aaauuaaaag aauucaaaaa agcacuaggc     1080

gaacacaacc ucgaacgaaa aacauuagau gaaauagcaa cacauauaac acucacaaaa     1140

gacgaaaauc aacuaaaaaa acaacuggaa caauacgcac ucucacagaa ucaaauagau     1200

gcguuagccg aacuggacuu caacgaccau aucaacuuau cauucaaagc acuauacgaa     1260

auacuacccc uaauggaaaa aggaaaaaga uacgacgaag cuugcaacga acuuggacua     1320

aaagcuaaaa ccagcaauca aaaaucagag uucuuaccag ccuucugcga uuccauauuc     1380

gcacaggaau uaacaaaccc aaucgucaac agagcuauaa gcgaguaccg caaaguacua     1440

aacagacuac uaaaaaaaua uggaaaaaug cacaaaauac acauagaauu agccagagac     1500

gcagguuuaa gcaaagaagc ccguaacaaa auagaaaagg aacaaaagga gaauaaagac     1560

auaaacgaca aagcagaaaa aacaugcaaa gaauucggau uaaaaccgaa cgcgaaaaac     1620

auacuaaaaa ucaagcugug gcgagaacaa ggagaaauau gcgcauacuc ugggaaaaaa     1680

auaacaauag acgaccuaaa ggaagacaag gcuuuagaag uagaucacau auacccauac     1740

ucccgguccu acgacgauuc auacaauaac aaaguccuag uauucauaaa agaaaaccaa     1800

cuaaaacuua aucagacacc auacgaagcu uucggaagca aucaagaaaa auggagcaaa     1860

auacaaguuu uagcacaaaa acuaccauau aaaaaaaaaa auagaauacu agaugaaaac     1920

uucaaaaaca aagaccagau agaauucaua ucaagaaauu uaaacgauac aagguaugca     1980

acaagccuaa uagcuaaaua uacuaaagaa uaccuagaau uucuaccauu aucggaugac     2040

gaagauauaa guuuaaaauc aggagaaaaa ggaagcaaaa cacacguaca gacuaugaac     2100

gguaugcuaa caagcguacu aagacacgca ugggguuucu cccaaaaaga cagaaacaau     2160

caucugcacc acgcacuaga ugccauaauu auagcuuaua guaccaacuc aauuauaaaa     2220

gcauuuucag acuucaaaaa aaaccaagaa auacucaaag caaaacuaua cgcuaagaaa     2280

uuaacaagcg aagaauacaa aaaccaaagc aaauucacug gauuugaaaa auacagaagc     2340

aaaauucuag aaaaacuaga cucaauauuu guaucuaaac ccccgaacaa aaacacuaaa     2400

ggugcacucc acgagcaaac cuuuuacagu uaucaagaca uacuggaaga auauaaaacc     2460

caagaaggcc uuaaaagggc acuagaaugc gguaaaguac gcaaaauagg uacaaaauac     2520

guagucaaug auaggauggu acgccucgau auauuuaaaa aagacaauaa auuuuacggu     2580

auacccauau auacuaugga uuuugcacua ggaguccuuc caaauaaagu aguuacuauc     2640

gggaccgaca aagauaaaau aaaaaaggaa uggaaaacaa uugacgaaac cuacgaauuc     2700

uguuucucau uauauaaagg ugacuuggug cuuauacaaa agaaagaaau gcaagaauca     2760

gaauuugcuu auuacaaugg auuugacaua ucuagugcaa guaucagccu cgagaaacau     2820

gauaacaaau uugaaaaucu aacagaaaau caaaaauugc uauucccaaa agccaaagaa     2880

ggugauguag aaguacgagg cauuggaaua caaggacuaa aaguauuuaa aaaauacaug     2940

guaaccccuc ucggggagau acaagagacc agaacagaag cacgacaaaa uauacaacca     3000

cgaaaaaaac acggacugag auga                                            3024


<210>  4909
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4909
augucugauu cuucauaugu acuagguuua gaccuuggaa ccaacagcau uggaugggca       60

uguguaagau uaaacaaagg aaaaccagau ggacuauuag cgcaaggcgu aagaaucuuu      120

gaagcaggau uggaaauaga cgcguauggc agagguacac cauugaacac agaaaggcga      180

aacgcacgac aaagaagaag auuacaugac cgacgaaaga ggagaauccu aaaagugagg      240

agaauacuac aagaagcagg aaugcuacca gcuaauaagc cagaucacaa agaugcugag      300

uggaaaaugu uacuugcacg uaauccauac agauuaagag ccaaggcuuu agaugaacaa      360

aucgacuuga aagagauagg auuaugcauu uaucaauucg cacacagaag aggauuuuua      420

ucaaauagca aaauauuaaa gaaagacgau gacaggggua aauuaaaaga ugcuacuaaa      480

gcauuugcug aagacaugga acaaaaagga gcucgaacau ggggagaaua cuuaucucac      540

uuagacccac acgaacaacg cauaagaaca aacuacaccc uuagagaaug guauaccuca      600

gaauuugacc uaauauggga aaaacaacaa ucauuuuacc cgcauguacu aacagacgac      660

uuauauaaaa gggucaagga agcacuauuu uaucaaagac cauugaaaag ccaagcccau      720

cuauuaggcc aaugcgaacu cgaaccagga cgcaaacgcg cacccuuugc uuuguuaaug      780

gcucaagaau uuagauuauu acagagcuua aaccaucuuc gagugguauc aagcacaggc      840

gaugaacgcu uauuaacuga cgaagaaagg aaacaagcac uggcauuacu uagaagcaga      900

ggaaaccaaa aauucacaac acuaagaaaa guccuaaauu uaagaggaac aaaauuuaau      960

uuagaacgag guggagagaa aacaauccaa ggaaauaaca cagcagcagc auugggaaau     1020

cuauuuggag aagccuggga ccauuuuagu ucugaacaga aaaaucaaau cguagaagac     1080

aggcuaacaa ucggugacac agaugcauua auacguagaa ugaagaaaac guggggauug     1140

gacuccguaa cugccgaaau guucagcgag accgaacucg aagacggaua uuguggauuu     1200

ucgcgcaaag cccuagaaaa auuauuacca cuucuagaga gagggcacgc cuuaauggaa     1260

gcgguacaag ccgcauaccc agauuaucag aaaaaucugc cacgaacaga uaccuuacca     1320

ccacuagaaa auuuaagaaa uccaauaguu caaagaaccc uaucugaacu uagaagagua     1380

gucaaugcaa uaauuaaaga auauggcaaa agugacaaaa uaagaauaga auuagcaaga     1440

gauaugaaac aaaccgccaa aaacagggaa aaaucauggg aucggaugag aguacgcgaa     1500

agacaaagaa aagaggcagc aaagaaaaua cuugaagaaa cagguaaucc cgcaccaagg     1560

agacaugaaa uugaaaaagu ccugcuagca gaagaaugca auugggagug uccuuauaca     1620

ggaaaacgaa uauguaugaa uacacuacuu ggagacgcuc cucaauuuga cauugaacac     1680

auuaucccau ucagccgauc auucgacaau aguuuuagca auaaaacuuu augcgacguc     1740

aauuauaaua gaaauguaaa aggcaacaaa acaccauacg agagcgcauc agaaacagaa     1800

cacgaagaaa uggugugccg agucaagaga uuuaccggag auuauaaaga gauaaaauua     1860

gauagauuua auaccagaga aacagacuca agggcagacu ucauagaaga auuuuccaca     1920

ucacaauuaa augacacaag auaugcuuca agacucgcag gagaauauuu aggaaaguua     1980

uauggaagug aauggcgaaa acauauccaa auugcaaagg gcgguacaac aaaauauuua     2040

cgugacgcau gggaucuaaa cagaauacua agcgauggag gaacuaaauc uagagacgau     2100

cacagacauc augcgguaga cgcugucgua guagcauuaa cuacaccaca aaccguaaaa     2160

gcccuaagcg acgcaaauca aagagcacca gagcauagaa gguacgcguu cucagauaug     2220

ccuuuaccgu gggagacauu uagacaagac gucgaacaag caauaaauuc aauaacagug     2280

agucacaaag uaugucgaaa agcaaaugga caauugcaca aagaaacuuu uuauggacuc     2340

aucaaagauc gaaaaaccgg ggacacaaga acaguacuga gaaagccguu acacgaacuc     2400

acagacaaag agauaaaaag agccgaaaua guagauagcu ccauacgaaa acuaauagaa     2460

caaaaauuaa aagauuuaga ccaaccgcca aagacagcau ucaaagauag aaauaaccau     2520

ccauucauca uugacaagaa cgguaauaag cgauucauac auaaaguaag aguguaccaa     2580

gaugcaaaac cggucaaaau ugcggaaaac agacacguca aauuaggagg aaaucaccau     2640

cuugaaauau uugaaguaau agauaagaag ggaaauccaa cauggaaaug ugaaguaaua     2700

uccuuauuug acacuagaca acgagucaaa gaaaagagac ccgucaucgu uagacaagau     2760

caaaauggaa auccauuaaa auucucauua uccaucggag aagcaguaca auuagacugg     2820

aacggaggaa gagaaacagc aguuguacaa aagaugagua aaacaccgaa uacugucgaa     2880

uauguauuua gaaaacacaa ugaugcucgc aaagcggcag acauaccaua uaaagaaaca     2940

aucacaguaa aaagcgauag cgcccucuuu gcaucaaacu uacagaaaau uguaauaacu     3000

cccacaggcc uuauacgacg agcaaacgau uga                                  3033


<210>  4910
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4910
augaaaaaua gaaauguaau aggcauugau guuggcacua acucgauugg augggcccua       60

guugaagaga acgaaaaugg uuuaccacaa ucucuaaaag cagcaggcgu aagaauauuc      120

caagaagcag uagaagcaaa aaccagaacu cccaaaaauc acaaaaggag acaagcaaga      180

cuagcuagac gaguucuggc gcgcagagca agaagaaaac aaagacuuca aaaucuacua      240

uuaaaacaug gacugcuccc aggaggagua acagacccug uccaaagaga agccgcuuuc      300

accacacuag gagauccuua ucaguuacgc gcaaaaggac uaaaucuccc guugggucua      360

caugaauuug gacgcguccu auuucaacua ggcgcaagga gaggauuuuu aagcaacagg      420

aaaacuagau auggacaccu uaauaaacua gacgaguuag cugauuuaau ugaagcagag      480

gacgcagcac ccaaaacugg agaugcagaa gaaggaauaa uucgaaaaga aauaggagaa      540

uuaagacaag cgaugucagc agccgguagc agaacccugg gcgaauauuu agccggcuua      600

gcgaaagaag aaaggaaaag augucgacau acagaacgug caauguuaga ggaagaguuc      660

gaacgucuuu ggaugguaca agcgagauuu cauccugaau ugagugacga acuaaaagua      720

caaguacaca cugugauauu cuuucaaaga ccccuuaaau caaaucgagc caguuuggga      780

aaguguagcc uagaaccauc aagaagaaaa gcagcaagag cauggccaga agcccaacga      840

uuuagguuac uccaagacuu aaauaaccuc gaaauauugg auccaagaac gggggaauua      900

agaagauuaa gagacacaga aagagcuauu uuguugaaug cucuagaaca acaaaaauca      960

augacaugga acgcuguaag aaagaaauua ggccuucauc aaggugaaaa auuuaauuua     1020

gaaaccgggg caaaagcuga aaccggucua aaaggacuac agacccuagc aucauuacau     1080

gcaaucuuac aggggugguu cgacgaacua ggagauaaaa gagaucugcu aauagaagac     1140

cuaauaacaa uagaagauag ugcaggacua uacagaagau uacgagagca uugggguuuc     1200

gauagagaaa cagcguucag auuagcgaca uuagaacuag aaccgggaua cgcaucccua     1260

ucacugaaag caaucagaaa auuauuaccg cauaugggag cuggccauag auacucggaa     1320

gcccgacaac aaguggguua cggauaugaa acaggacaaa gagauuuaca agaaagauua     1380

ggagaaccac cagauccuag aaauccagua guagauaaag cccugcacga acuacgccga     1440

guaauaaaug cauuaauaca uagauuugga agacccgaug uaguaagggu agagcuggca     1500

agagaucuga aauuagcaaa acgagacaaa gaaagacucg aaaaacaaaa uagagaaaac     1560

cagagacuga acagaaaagc agaagcagca cuagcagaau uagguuuagg acagcccuca     1620

cgagacgacc uaauuaaaua uagacuuugg gaggaaucga aaggccuaug ucccuauacc     1680

ggaagagaaa uaggccuaag uaccuuauuc aguccggaag uagaugucga acacauuaua     1740

cccuacagua gaugccuaga ugacucauac augaauaaaa cauuaugcau gacagaagaa     1800

aaccgaaugc agaaaggaaa ccgcacacca ugggaggcau acggcggcga cccagcaaga     1860

uaugaacaaa uauuacaaug cgcgagauau uuuccuaugg ccaaaagaaa aagauucuua     1920

gaacagggcu cagauuuugu ugaccaauuc auacaaagac aauuaaauga cacgagauac     1980

auagccaaau uagcgaaaga auaucuagca gcugucugua ccaaugucca aguaacaaaa     2040

ggaggaacaa cagccaugcu aaggcaucac uggggauuaa auaggcuauu aggagaggau     2100

gauggucaaa aagagcgagg agaucacaga caucaugcac uagacgcagc agucauugca     2160

gucacuucac gaagucucua ucaaagacuc gcucgcaucg cacaccaaag aggucaugcg     2220

ccaagucaaa ggggguuuua uauagaccca ccuuggcaag gauaucaagc ggaucuagcu     2280

caaacauugg ccggaaucgu uguaucccac gcuacuaaua gaaaauuaac uggagcacua     2340

cacgaagaaa cagccuacgg ucacauaccg cauguagguu uuguuuauag aaaaacacuc     2400

aacaaagauu ucaaacucgg ggacauagaa gcuauauacg acccaucauu acaagaauua     2460

gucaaagcgc auuuagaaca ucacggaaau gacccuaaaa gagccuuugc cgaaggaauc     2520

caaugcuauc auaaagacgg ucacacaccu auuaaacgag uaaggauucg agcaaagcaa     2580

guaucagaaa aaggaacauu uaguauuagc gauagggcug gcagagccua uaaacaucau     2640

aagacaggaa gcaaccauca ugucgaaauu uuaagacaca gagcgacugg aaaaauagaa     2700

ccaguauauc ucacuacaaa agaagcuaca gaaagagcac gcagaggggg cgaacccguc     2760

auacaacgug aucauggccc agaaugggaa uuuauaauaa gucucugcau caaugauaug     2820

guacgacuag gagaaggagg acaagucgcg uacuuucgcg uacagaaaau ugaagcaacg     2880

aauaauaggg ucacccuuag aagucauuua gccgcuacuc uagacgacga agauaugaaa     2940

guuacaacau caguuccuaa gcuagcagua augcccggcu uccaaaaagu auuaguuaca     3000

gcaguuggag aaguuuuaga acuucacgau uga                                  3033


<210>  4911
<211>  3033
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4911
augaagagaa uggcagcagu cggacuuaug ccagcagauc cuacagaagc aaaagcacuc       60

gaauuacuag auccauaugc acuaagggcg gcaggcuuag aucaggcccu gccucuaacc      120

cacuucggga gagcauuauu ucacuuaaau caaagacgag gauucaaaag uaauagaaaa      180

acggacaaag gagauaacga aagugggaaa auaaaagaug caaccaaaag acuagaugaa      240

gaaauggcua uuaagaacgc aagaacauau ggugaauuuu uacauaugag aagaacauca      300

gcaaccgauc cgaaaaaugu accaacaguc agaacaaggu ugucgauagc acgaagagau      360

aacgccgaaa aagaagaagc cgguuaugau uuuuacccag accgaagaca uuuggcagaa      420

gaauuugaua aguuauggaa agcgcaagca ggauacucac cagacguacu aacagacgaa      480

uuaagagaug aaauaagugu aauaauauuu caucaaagac cauuaaaaac gcccgaaguc      540

ggacuauguc uauuuuccgg cucacacggc guaccuaaga aagauaaacg gauaccuagu      600

gcacauccau uaaaucaaag acgaauacua uuugaaacaa uuaacaauuu acgcguagca      660

gcaagaggag aaccagccag aggucuuaca agagaugaga gggaugcaau aguacacgca      720

uuagauaaua aagcgcauac aaaaucacua ucaggaaugu ccaugaaacu aaaggcauua      780

ggaaaaguaa uaaaauuaag accagaacaa aguuucacuc uacaaacggc gaauagagaa      840

acaauugcau gugauccagu aagagcaagc cuaagucauc cagaucgauu uggcccuaga      900

uggaguaugc uggaugcuga cgcacagugg gauguaguac aaaggauaag agcaguucaa      960

ucagaagccg aacacgaugc uuuaguaaaa uggcuaaugg caacacacgg guuagauaga     1020

gaccaugcag aaaguacugc aaaagcaccc uuaccagaag gauauggaag acuaggauua     1080

acugcuacaa aaagaauacu cgcugcacua gaagcagaug uaauaccaua uucagaugca     1140

guaaaggcau gcggauggca ucacuccgau ggacgaacag gugaaguacu uacagaauua     1200

ccauauuaug gucaaauauu ggauaggcau gugauaccag gaacauacaa cgaaucugau     1260

gaugacguua cgagauacgg uaggauaaca aauccgacug uccauauugg uuuaaaccaa     1320

uuaagaaggc uagugaauaa aauaauaaca guauauggua agccagauga gauuguagua     1380

gagcuugcaa gagaccuaaa auuaagugaa gaucagaaaa gggauguaca aagagauaua     1440

aagaaaaaua cagacgcagc cauagcgaga ggugcgaaau ugucagagau ggguguaccc     1500

gauacuggag caaauaggau gguacugaga uugugggaaa guuuggguga ugacguaaug     1560

acacgcaauu gcccuuauuc aggcaaaaga auuucagcca caauguuauu ugauggcagc     1620

ugcgauguug aucacauuuu accuuacuca agaacacuug acgacggauu ugcuaacaga     1680

acacuuuguc uaaaagaauu uaauagacaa aagacagaua agacaccaug ggaagcaugg     1740

ggagauacuc cagcaugggc gacaauagaa gcuaaccuca aaaaucuacc ggcaaauaag     1800

gcuuggagau uugcaccaga ugcuauggaa cgauuugaag gagaaaauga cuucaccagu     1860

cgugcacuaa aagaugcaca auaccuuucu aggauagccc gaacguaucu ggacgcacua     1920

uaugauggag cagauggaaa aagucaugua uggguuguac ccggaagacu gacugaaaug     1980

uuaagacguc acuggggauu aaauucguua uuaccagaua aagacggugc aguaaaagcg     2040

aaaaauagaa cagaucaucg gcaccaugca auugacgcag caguagucgc agcaacagau     2100

agaucuuuaa uccaaagaau aaguaaaaug gcgagaaaag augaagugaa cggagcagaa     2160

gaaguagcua gaucgguucc uccaccgugg gaaggauucc gcacagauau agaaacucaa     2220

uuggaccgga uaauuauaag ucauagagca gaccacggaa gaauagaccc cgcagcaaga     2280

aaagacggac augauucuac aucaggucaa uuacauaaug acaccgccua cgggcuugga     2340

gaagguaaca gcgugguuuc cagaaaacca uuauugagcu uaaaaucuaa ugauauagaa     2400

gugacaacaa aaggcgcgaa uauaagggau guagacuuac aacgucauuu agagagaaua     2460

acaagaggau uagacggaaa agcccuggaa gcggcauuag cagaguucgc ugcaacugcg     2520

aaacuaccag acggaacuga uaauccauau uacggcuuaa gaagaguacg ucuagaagaa     2580

acgcuccaac aaggagcaag aguggaagua aaagauagaa acggaaaacc auucaaagcc     2640

uauaaaggug auucaaacca uuguuaugaa auauggagga ugccagaugg aaaaguagua     2700

ccucaaguua uaacaacaua ugaagcacau cagaucgaug uugaaaagaa accacauccg     2760

gccgcaaaaa gauuguuacg uauauuuaaa agagauaugg ucgcgauuga acaagaugac     2820

aaagcaauua uuggguacgu ccaaucgaug caugucgcca auggccuauu uauugccccg     2880

cauacagaag cuaaugccga ugcaagaagu agagauaaau cggauaguuu uagguuaauc     2940

caaaugucag caggaccgcu aguaaaagca aaugcaagaa gaguuuaugu agaugaaaug     3000

ggaagacuaa gagauccagg cgccccauca uga                                  3033


<210>  4912
<211>  3039
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4912
auggcaaaua ugcguuacag auuggcgcuu gaccuaggaa gcacaucauu aggaugggcc       60

augguaagau uagacgccca aaacgaaacc ugugcaguaa uaaaagcggg aguaagaauu      120

uucagcgaug gacgaaaccc gaaagacgga aguaguuuag caguaucacg uagagaagca      180

agagcaauga ggaggaggag agauagacug cuuaaacgua aaacaagaau gauggcaaca      240

cuaaugaagc acggcuucuu cccacaagau gaagccgcua gacaagcauu aguaaauaag      300

gaaccauucg cccuaagagc aaaaggacua gaccaugcac uacuaccuga agaauuugca      360

agagcacuau uucacauaaa ucaaagacga gguuuuaaau cuaaccguaa aacagauaag      420

aaagaaaccg acaguagugc ucuaaagaaa gcaauaggac aacuaaggga agcuaugcaa      480

gcaucaggau guagaacagu cggugaaugg uuaugugcua gacaucaaca aggcuuauca      540

guucgugcca gauaucgaga aaauagaaua acuaaagaug augguaaaac aaaaauagag      600

aaaucauacg acuuauauau ugaucgagcg augauagagg cugaauuuga ugcccugugg      660

gcaaaacaau cagaguuaaa uccagaguua uuccacgaaa cugcucgugc agaguuaaaa      720

gauugucuau uacaucaacg accgcuaaaa ccaguuaaac cuggaagaug uacuuugcua      780

ccagaugagg aaagagcgcc acuugcgcua ccgagccaac aaagauucag aauauaccaa      840

gagguaaaca aucuuaggcu acuacgagaa ggauuaaaug aagaaccauu gacauuaaca      900

caacgcgauc aauugauaca ggcuuuagaa acaaauccaa aacgaucauu uacacaaaua      960

caaaaauuau uaggauuuag uggacaauuc aaucuacaag acgauaaacg uacagaauua     1020

aaaggcaaua gcacaagugc uaugcuuagc aagaaagaau uuuucggauc acaauggcua     1080

ucauuugaug aaacucauca agaugaaaua guaaugcaau uaguaagcga agaaaacgaa     1140

gcacgguuaa uagcuuggcu acaagcagag acaggagucg acgaaaucac ugcagagaga     1200

auagcaaaua ccccauuagc agaaggauau ggaaguuuau cagcaaaagc auuagagaaa     1260

auccucccag ccuuacgagc agauguaaua accuaugaua aagcuguauu agcagcugga     1320

uuugaucauc acagucauau aucacaugca gcaacaggug agaucuuacc agauuuacca     1380

uauuacggac aauaucuaca aagacacgua ggauuuggua caggcaaacc ugaggacccu     1440

gcagagaaaa gauaugguaa aauagcaaac ccaaccguac auauaggauu aaaccaagua     1500

aggaccguug uaaaugcacu uauaaaaaga uacggacauc cuagcgaagu gguaguggaa     1560

uuagcacgcg aucuaaaaca aagucaagaa caaagaaaag aagaacaagc acaacaagcu     1620

aaaaaucaaa gaagaaauga gaggaugaga caagauauag caggccuauu aaacacaagu     1680

accgagagag uaagggcauu agauuuacag aaaaugauuc uuugggaaga acuaucguuu     1740

aaugucgcag acagaagaug uccuuauagu ggugugcaaa uaagugcaca aaugcucuuu     1800

agugaucaag ucgagauaga acauauccua ccuuuuagcc aaacacuaga ugauucauua     1860

aauaauaaaa cuguagcaau gagacaagcg aaccgaauaa aggguaaucg aaccccagca     1920

gaagcagcag ccgauuuugc agcacaaggc uggccugucg aagauauguu acaucgugcu     1980

ucuuuaaugc caaaagggaa acgauacagg uuugcucaag augggauggc acaaugguua     2040

agagaagaua aaggcuuuuu agcaagagca cuuaaugaua cacgucaucu aagcagagua     2100

gcacgagaau auuuaaguuu agucugccca caaggaaccc gaguaauucc cgguagaaug     2160

acugccaugc uaagagcuaa auucggucuu aaugauauac uuggccuuaa cggugaaaag     2220

aauagaaaug accacagaca ucaugcagua gaugccugug uaauugcggu uacggaucag     2280

ucgauguuac aaaaauucgc aaaagcauca gccucagcaa gggaaaagca acuaaauaaa     2340

uuagucgaau caaugccauu accuugggac ucguauaggg accacguaca aagagcagug     2400

gagcgaauau ggguaucuca uaaaccagau cauggacaug aaggagcaau gcauaacgau     2460

acugcauaug gacucuuagg uggaggccac guucaaguua gaaaaaggau ggauggugug     2520

agaacacgag aaacagcugc auuaaaaguu auagaguucg cugaccgaca agcacaucca     2580

cgacauggau uauugccaaa cggagaacca augccauaca aaggauauaa aggcgauuca     2640

aauuacugua uggaaauugu ucgaaaugau aaaggaaaau gggaaggaga aguagucagu     2700

accuuugaag cguaucaacu gguaagacaa caugguuuag caagacuccg acauccggcg     2760

uuaaguguau cagggaggcc acuuguuaug cgacuauuga ggaacgacac auuaagagua     2820

auacacaacg gugaacaacg aacaguaaga auaugcaaaa ugacggcgga aggaguaauc     2880

gcauuagcag auaccacaga aucgaacgug gaugcaagaa cgagaaccaa agauuuauca     2940

uauauauuua aaaccgcugg aucacuucag aaaaguaaag guagacgggu ugcaauaagc     3000

ccuucaggag aaaugaaaga uccuggauuc uucgacuga                            3039


<210>  4913
<211>  3051
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4913
auggaagucu uuguagaugu aagcgcaagg caauuaggca uagauuuagg uauaggaaca       60

uguggcuggg cucuccuggc ugaagauuca uuaauagccu uagguacaag aacauuugau      120

guuccagaaa cagauaaaga aaggaucccg accaaccaga uccgacguca agcacguggc      180

augagacgag uaauagaucg aagacgacaa agaaugaacg cugucagaag acuguuggca      240

gaucaugguc ugcucgcaga ugggggaaaa uuaggccucg caacaguuag acucgaucca      300

uggcaagcaa gagccgaagg ucuagaucgu uuacuuagug cuggugaauu agcaguugca      360

cuaggacaua uagcaaaaca ucgcggauuc agaaguaaua gcaaaaggga ccgaggcgcu      420

aaugcuccua aagaaucguc ugccaugcug ggagcaauug cacaaacggg agaacgacuc      480

caacauuggc gaacgguagg agaaauguuu uggaaagacg aauuuuacca aacaagacga      540

agaaauagag auggagauua uaguagaacu guacaaagau uagauuuaga aacagaaguu      600

cacagguuau uagcugcgca acgacgccug ggaaacccau uagcaacauu ugauuuagag      660

caaaguuuua uagacguagc auucuuucaa aggccucuag cggacagcga ugauaagguc      720

gggccuugcc cauuugaacc uucagagaaa agggcugcaa gacauucgcc gucguuugaa      780

cgauuuagac uguuaagucg auuagcugca cuaagacuau caaguggugc uggagaaagg      840

ccauuaagcg acgccgaguu cgcagcugcc ugugcagacu ucggaaggcu acaaggauuu      900

ucauucaaaa gauuaaggca gcauuuaaaa cuagauccgu cuaucagauu ugcaggaaua      960

gcacccgaug aagaaggaaa aagagaugua guagcuaggu cgggagaagc caccccggga     1020

agugcagcau uaagacauug uuugggggac acaguuuggg auaauaugcu uggaacacga     1080

cccgcauuau uagaugcaau agcagccaua cucacauuuc gagaugaugc agauagaaua     1140

gcggaaggau uagcagguuu aggaauagaa gaaccgauuu uagaugcauu aauggauggg     1200

guuagaaacg ggucauuugg aaaauuuaag gggccaggac auauaucagc uaaggcaugc     1260

agagcauuac ucccccauuu agcacgaggg agaguauauu cagacgcaug cgcugaugcg     1320

ggcuaugauc acaguaggca aacugaaaca aauuuagacg auauaagaaa cccaguagca     1380

aggaaagccu uaggugaagc auuaaaacag auaaagauua uaauccauac acacggaagg     1440

ccaacagcga uacacguaga aauggcaagg gauauaggua aaucagcaga ugaaagagac     1500

gaaauaaaga aaggaauaga uaaaagaaac ucagaaaaag auagagccag gcagcaauuc     1560

cacgaaaugg uuggaacucc agcagccggu gcagaagauu uguuacgcuu ugaacucugg     1620

aaagagcaaa auggauggug uuuauacaca ggaagagaga uaccaauuga ugcacuggug     1680

ucaucagaca aucgaauaca gguagaucau auauuaccau ggagcagaag uggagaugau     1740

ucauauauua auaaaacacu augcuucacu gaagcaaacc aacacaagaa agguaugaca     1800

ccaugggaau gguuacacga ugacccagaa cguugggaaa aauucgccag aacuguugaa     1860

ggcuguaaaa cuaugaaagg aaggaagaaa agaaauuugu uauuaagaga cucgucuaga     1920

uuagaaaaag aguucagauc ccgaaauuug aaugauacac guuaugcaug uagauuauua     1980

uuaggaauau uaaaacauac auauccugau auaguggucg ccgccaggcc uggccccuua     2040

acagacagau uacgacgagc uuggggacua caaucacuaa agaaacauga agauggaacc     2100

cgaaaagaug augauaggca ccaugcucua gacgcugcag uaguggcagc aacaucacaa     2160

aguauguuac aacgauuaac aacacuauuu caaaaagcug aaagagaagg uggaagaagg     2220

gacuucguag gacuggaacc uccauggcca gguuucaggg cagauuuaga agcaaaauua     2280

gaaggggugg uaguaucgcg ggcggaacga agacgagcaa gaggugcagg acauaaagac     2340

acgauaagag ccauaggaga aggugacgcu ggcccaguag uguacgagag aaaacccgua     2400

gcagcuuuaa cagaaaaaga uuuagauaga guaaaggacc cggaaagaaa uaaaguguug     2460

aucgaaaauu uaagacaaug gauagcagcg ggaaaaccua aagaucccga caaggcccca     2520

cgaagcccaa aaggcgaucc aaugacuaag guaaaauuag uaacaacaga ugacguacga     2580

aucuuaguaa gaggcggaac agcugauaga ggggacaugg cccgaguuga uguauuuaga     2640

caggcggauc caaaaggaag auggagguuu uucuugguac caguauaucc acaucaagua     2700

uuugacccua cugauuggcc ucaaccuccu aauagagccg ugguagcaua uaaaccagag     2760

aaagaaugga cgguuauggg accggaccau gaguucuuau gguccuuaua cccauuuuca     2820

uggauugcgg uagaaaaagc agauggaaca agaaucgaag gauacuuuag agguaugcau     2880

cguucuacgg gagcaaucuc cauaucgccc caccauagca agagcuuagc aucaaccgga     2940

auaggagcaa aaacccuagc aaguuuuagg aaauuugcaa uggacagaau ggguaacuua     3000

ucagaaauag aaagagaagu acgaacuugg caugggguag cauguacgug a              3051


<210>  4914
<211>  3054
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4914
augagaguag auucauuagg aagguguuua ucagaugaag aaugggcuag aguuauauac       60

cauuuaugua aacauagagg auuucauugg auaucaagag ccgaagcuaa acaaacagaa      120

ggagacagua aaucagaagg agggaaaguu aaacaaggcu uagcaggaac agcaaaacua      180

auggacgaaa aagguuaucg uagcgcagca gaaaugguau uagccgaguu cccagaagca      240

cagaggaaua aacaaggaga auauagaaaa gcucuaucaa gagaacuacu agcaaaagaa      300

uuagcacucu uauucgccag acaacgaguu uuuggaaaca gacaugcaac ggacacauua      360

gaaacagcua uacuagguaa uucagauaag aaaagcggcc uauuuuggca acaaaaacca      420

gcucuagcgg gugcagauuu auuaaaaaug cuaggaacau guacuuuuga gaaaaaugaa      480

uaucgugcac caaaagcgag cuuuacggca gaaaggcaug uguggcuuac aaggcuuaau      540

aaucuacgaa uaguaacgga cggaauaaca cgacaauuaa cagaacagga acgacaauua      600

guacuaccac uucccuaucg acaagcaggg gauuuaacuu auaaacaacu aggaauggca      660

uuaacaaaag caggauuacu ugaagcagga uguuuuaaau uuauagguuu agcguaccca      720

accgaagcgc aaaaagcgga agguaaaacu aaagauccag aaagcgcgac ccucguaaaa      780

auaccggcau ggcaagaauu aagacaaaca uuaaacaaag ccagucuaaa ugacgaaugg      840

gaaaaacuuu caggagaugc ccuuugcggu cagccagaaa uauuugauaa aauagcuugg      900

auacuuucag uauacaaaga ugaugacgaa guacgaacag aauuagccaa auuaguacug      960

cccaacccag aaggacuaac cgaagcacua cuuaauauaa gguuugauaa auuccaugca     1020

uuaucacuca aggcuuuaag ggcaauaguc ccacacaugg aaaaagguuu aagauaugau     1080

gaggcuugug uucaagcggg uuaucaccau ucacaauuac acaaaauagg agaaggcgaa     1140

cauaaauacc uaccuccuuu auacgauggu agagacggag augguagaau gauuuuugcg     1200

gaagaugccg auauaccacg aaauccagua guauugcgaa guuugaauca agccagaaag     1260

guaguaaacg cgauuauuag acguuacgga uccccuucag ccguacauau agaaauggca     1320

agagaucuca guagaccauu ugacgaacgu aacaaaauca agaaagauca ggaugaauau     1380

caagcaagaa aucaaagaga uagggaacaa uuuauagaau uguuuggaca uccaccuaaa     1440

ggacuagagu ucgaaaaaug gcgacuauau agagaacaaa auggacaaug ugcauacuca     1500

caaaaacccu uagcaagcag uggagauguc gcugaaauuu uucuugacgg cucuacagaa     1560

auagaccaug cauuaccaua uucuagaucu uuugaugaca guaaaaauaa uaaaguauua     1620

guacuuacac gagaaaauag agauaaagga aaccgaacac cguaugaaua ccuuggagga     1680

gcagaaaacu cagagcguug gcaaagauuc uuuaguuuug uagaauccaa uaaagcauac     1740

cgacuugcaa aacgaacgcg ucugcugaga aaagauuuug gagcaaaaga aucagaagag     1800

uucagagaac gaaauuuaaa cgacacacga uaugucuguc gcuucuuuaa aaacuaugua     1860

gaacaacauc ugaaacuugc ugaaggauca gaagccaaaa gguguguagu cguaucagga     1920

caacugacaa acuuucuacg aaccaggugg gguuuauuaa aaauacgaag cgauucugau     1980

cgacaucaug cauuagacgc cgcaguagua gcagccugcu cacacuccau gguacaaagg     2040

cuaagcaacu auaguagaac acgagaacug gagcagguac gagaugguuu ugucaauauc     2100

gaaacuggug aaauaguuaa uccugcuaug caccaacaac uccgugaaca uuuuccugau     2160

ccauggccgu auuuuagaca ugaauuagaa gcucgacuaa aaauagauag uccagcuuua     2220

uuacgcgaag aaauggcaag acugucuagc uacacagaag aagaacuagc agcacuaaaa     2280

ccacuguuug uaaguagggc accucaaaga agaaauggug gugcugcaca uaaagacaca     2340

auuuauggac aaccagaaaa acuaaagaaa aacgguucug uaucacaaaa aguugcacua     2400

gccucgcuug cguugaaaga caucgauuua uuggccgauc cccaucgaaa ugcgaaauua     2460

uauggagcaa uaagggaaag acuagaagcc cauggaggaa aaggggauaa agcauuuccc     2520

gccacaaauc cacucaggaa accagacaaa gaaggcaauc caacaggucc aauuguuaga     2580

acaguaacua aagucaucga uaaacuaagc ggaaucccag uccgaggagg aauugccaaa     2640

aaugacacaa uguuacgagu agacguguuu acaaaagcag gcagauucca uuuagugccu     2700

guuuauguuc aucaucgagu caccggauua cccaaccaag caaucaccag agguaaagau     2760

gaaaaucaau gggcgauaau ugaugauaca uucgaauggc gauuuucauu auauccuaau     2820

gauuuaguac aaauaagacu caaagaugaa acacaucucg gcuacuaugc aucaacugau     2880

agaaguacaa guucaauauc aauuugggcu caugacagaa accagucagu aggaaaagau     2940

gguuuaauuc gaagcauagg aguaacaaca gcgaaagaca uugagaaauu ucauguggau     3000

acguuaggaa acguauaccc ugcuccccca gagaaaagga gaggacucgc auga           3054


<210>  4915
<211>  3066
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4915
augagaagaa gaagagauag guuuaaacag agacaggcag cguuauuaaa acauuuggca       60

ggaguaggau uauuuccagc agacgaagcu gcacgcagag aauuagcugg uuuggauccg      120

uaugagcuaa gagcgagagc acuucaugaa gcacuaccac ugaaccauau cgggagggcu      180

cuauuccacc uaaaccaaag acgggguuuu aaaucaaaca gaaaaacuga cagagguaaa      240

gcugaugaug aagguaagau cagacuaggc guugcacgau ugcaagaggc aaugcaugaa      300

aaaggcagug cgacauuagg agaguucuug cauggaagac gagcagcagc aacagauccc      360

aaucaaauuc cuagcguuag aacaagauua agagcagaaa guggcgaagg cgcaagagga      420

gaugguuaug auuuuuaccc agauagaagg auguuagagg acgaauuuga agcaauaugg      480

gaaucacaac aagugcauca cccagcaguu cuuacugaug aaauaaaaca aagauucuuu      540

gaaguaauau uuuaccaaag accgcuaaaa caaccagaaa uagggacaug uacacucgua      600

gcuggcgaaa gaagauuacc aaaagcucau ccacuauuuc aaaagagacg auuauuagag      660

gaacuaaaug cuuuaagaau cguuuggcca ggaguacaag ccgaauuguu aucacgugaa      720

cagagggacc uagcauaccu gaaauuaaaa gauaaaaggg caguuagauu ugaaucacua      780

agaaaagcac uaaaauugga uccugaugca agauuuaaua aagaaaguga aaaccgaagu      840

gaacuaaagg gugaugaagu ggcagcagaa cuaggagcca aaucgcguuu uggaggaaga      900

uggggacaca cggcaccagc ggaccagugg gaaauaguuu ccagaauaca agccuuggaa      960

agugaugaug acgaagcaca auucuguguu uggcuaucug aaagauaugg ucuagauagc     1020

gagcaagcaa gggcaguagc agcagcacga uuaccacaag guuucgggcg uuuugggcuu     1080

acugcaacua cccgcuuaau uaaggcuuua caagacgaag ucauuguaua cucagaagca     1140

guacaaaaga cuggacuugg gcaccauagu gacuuucgca acggagaagu auuggcagaa     1200

cuccccuauu auggaaucgc gcuagaaaga cauauaaugc cagguacagg agauccugga     1260

gauagcgaug aaaugagagu gggucgagua acaaauccca cgguucauau aggauuaaau     1320

caauuaagaa gaguaguaaa ccgauuaaua aaaagauacg ggccucccag acagauagca     1380

guugaauuag cacgugaauu aaaacuagau gaucaacaua aaagggagau aaauggaagg     1440

aauagacaaa auagggaagc agcggaacga agaagucaga aauuaguaga uauagguauu     1500

gcagacaaag gagcaaacag ggcauuauug aaauuauggg aagaacuaaa uccagauaau     1560

gccuuggaua ggagaugcgu guacacgggg agacaaauuu cuauagaaau guuauuuagu     1620

ggggcaguug aaguugauca cauauuaccg uuugcagcaa cgcuagacga cagcaaugca     1680

aauaaauuau uaugcuugag ggaagcaaac cggaacaaaa gaaggagaag uccauucgaa     1740

gcauuugguc agggagcaga uugggaagaa auagcagcac gugcugcuag guuaccgagg     1800

gaaaaacgau ggcguuuuga accagaugca auggcaagau uugauacaga agagaaauuu     1860

uuagcacgac aauuagucga cacucaguau uuaagucgcc uggcaagaga auauuuaggc     1920

guauuauuuc ccgacaaagg agaggggucu ggacgaguau ggguuagucc aggaagacuu     1980

accgagaugc uaagaagauc auggggauua aaucauuuac ugccggauca caaucuagga     2040

ggaggagcag aucaaccuaa aaauagaagg gaucaucgac accacgccau ugaugcaaua     2100

guuguugcag uaaccgauag acauuuacua caagcuauag cgagagaagc aggacaaaga     2160

ggacaugagg cagccaauag gaugacagcu gaucuaccag uuccuuggcc agacuuugau     2220

agagauguug ggcaagucau aagacagguc gucguaaguc aucgcgcuga ccaugguaca     2280

guuucaaagg caggagccgc gaaaggacaa gacucaacgg cuggcagacu acauaaugac     2340

acugcauacg gccuaacagg acaaaccgau gaaaaaggca auacgauagu gguaaggaga     2400

gucccguuaa cugcauuaaa gaaaccuaca aguaucgagg cuguacgaga ugaugaauua     2460

cgaaaagcau uaagugucug gacuagagga uaugauggaa aagccuuuga agcaagauua     2520

agcagauuuc cggaacuagg accauuacag uuuagaggaa uaagaagagu uagagucguc     2580

gagccucuua ccguaauucc aauccgagau agcagugguc ggguauacaa aggauauaaa     2640

ggugacucaa auuacaggua ugauguaugg gaacuuaaag augguaaaug gguagcagaa     2700

guagugucaa uguucgaugc ucaucaaagu ucuuggacaa guccgguaag aagagaaaau     2760

ccuacugcgc gaaagguauu aagucuacaa caaaaugaua uuguggcuau ugaaccugaa     2820

ggaaaagaaa gagaauuaau gcgaguggca caacuuagac aaggugguca gauaaccuua     2880

gcagcguguc acgaagcagg ggauuuaaag cgaagagaug cggaaaguaa ugaagucgau     2940

ccuuuuaaau auuuuagucc aacagcaggg ggucuaaaga gagucagagc uagacaagua     3000

agagucgacg aauuaggacg aguacuagau ccuggaagua gagacagaga gcguggggca     3060

gcguga                                                                3066


<210>  4916
<211>  3066
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4916
augaaauaua cccugggacu agaugucgga auagcaucag uaggaugggc agucauagau       60

aaagacaaca acaaaauaau agaucuagga guccgauguu uugacaaagc agaagagucu      120

aaaacaggag aaucacuagc uaccgcuaga cggaucgcca gaggaaugag aaggagaauc      180

agucgacgau cacaaagacu acgcuuaguc aaaaaacuau uuguucaaua cgaaauuauc      240

aaagacucaa gugaauuuaa cagaauauuu gacacuagcc gagauggaug gaaagaccca      300

ugggaacuaa gauacaacgc gcuaucacga auauuaaaac ccuacgaauu aguacaaguu      360

cugacacaua uuacaaaaag acgaggauuc aaaagcaacc gcaaagaaga ccuuagcacu      420

acaaaagagg ggguagugau aacgucaaua aaaaacaacu cugaaaugcu cagaacaaag      480

aauuacagaa cuauaggaga aaugauauuc auggagaccc ccgaaaacag uaauaaaaga      540

aauaaggucg acgaauauau acauaccaua gcgagagaag aucucuuaaa cgagaucaaa      600

uacauauuca guauccaacg aaaacuaggu ucaccguucg uaaccgaaaa acuugaacau      660

gauuucuuaa auauauggga auuccagaga ccauucgcau caggagauag cauccuaucc      720

aaaguaggaa aauguaccuu auuaaaagaa gaacuuagag cacccaccuc augcuauacc      780

ucagaauacu ucggucuguu acaaucgauu aauaaucucg uucuaguaga agauaauaau      840

acacugacau uaaacaacga ccagagagcu aaaauuaucg aauaugcaca uuuuaagaau      900

gaaauaaaau acagugaaau aaggaaacuc uuagacauag aaccagaaau acuauuuaaa      960

gcacacaacu uaacccacaa aaauccaucu gggaauaacg aaucaaaaaa auucuacgaa     1020

augaaaucau accacaaacu uaaauccaca uuacccacug acaucugggg aaaacuccac     1080

uccaacaaag aaagccuuga caacuuauuc uauugucuca cggucuacaa aaaugauaau     1140

gaaauaaaag auuaucucca agcaaauaac cuugacuauc ucauagaaua uauugccaaa     1200

cuaccuacau ucaacaaauu caagcauuua ucauuaguag ccaugaaaag gauaauacca     1260

uucauggaaa aaggauauaa auauagcgac gcuuguaaca uggccgaacu agauuuuaca     1320

ggaucuucaa aacuagaaaa augcaacaaa cuaacuguag aaccaaucau agaaaacgua     1380

acgaauccag uaguaauccg ugcacugaca caagcaagaa aagucauaaa ugcaauaaua     1440

caaaaauaug gacuaccuua cauggucaau auagaacuag caagagaagc cggaaugacg     1500

cgccaagauc gcgacaauuu aaaaaaagag cacgaaaaca accgaaaagc acgagagaaa     1560

auaucagauc uaauaagaca gaauggacgc guagcaucag gacuagacau ccuaaaaugg     1620

agauuauggg aagaccaagg aggaagaugc gcuuauagcg gaaaacccau accaguuugu     1680

gacuuacuua acgacucauu aacucaaaua gaccauauau acccuuacuc aagaucaaug     1740

gacgacagcu acaugaauaa aguauugguu cuaaccgacg aaaaccaaaa caaacgaagc     1800

uacaccccau augaagucug gggaucuacu gaaaaauggg aagacuucga ggccagaaua     1860

uacucaaugc aucucccgca aagcaaggaa aaaagauuac uaaacagaaa uuucaucacu     1920

aaagaccugg auucauuuau aucacgaaac cuaaaugaua cacgcuacau aucaagauuc     1980

uuaaaaaacu acaucgaauc auacuuacaa uucagcaaug auucaccuaa aagcugcguc     2040

guaugcguua acggccaaug uaccgcucaa uuaagaucca gauggggacu aaauaaaaac     2100

cgggaagaga gugacuuaca ucacgcuuua gaugcagcag uaauagcgug cgcggacaga     2160

aaaauaauca aagaaauaac aaauuauuau aacgaaaggg aaaaccauaa uuauaaagua     2220

aaauauccuc ugccauggca cuccuucaga caagacuuaa uggaaacucu agccggagua     2280

uuuauaucaa gagcaccaag aagaaaaaua acgggaccgg cacacgacga gacaauaaga     2340

ucacccaaac acuucaacaa aggacuuacc ucaguaaaaa uaccuuuaac cacuguaacc     2400

uuagaaaaac uugaaacaau gguaaaaaac acaaaaggcg gcaucuccga caaagccgua     2460

uacaauguau uaaaaaaccg acuaauagaa cauaacaaca aaccccuaaa agccuucgcc     2520

gagaaaaucu acaaaccacu uaaaaacgga acaaauggag ccaucauaag gucgauacga     2580

guagaaaccc cauccuacac aggaguguuc aggaaugaag guaaaggcau aucagauaau     2640

ucacucaugg uaagagugga uguuuuuaaa aaaaaagaua aauauuauuu aguaccaaua     2700

uacguagcac acaugauaaa aaaagaauug ccauccaaag caauaguacc cuuaaaaccc     2760

gaaucacaau gggaacuaau cgacaguaca cacgaauucu uauuuucccu uuaccaaaau     2820

gacuauuuag uaaucaaaac uaaaaaggga auaacagaag gauacuaccg aucaugccau     2880

agaggaacag gcagcuuauc acucaugccc cauuucgcaa acaauaaaaa cgugaaaauu     2940

gacauaggcg uucguacagc aauuucaaua gaaaaauaua auguagauau acuuggaaac     3000

aaaucuauug uaaaaggaga accucguaga ggcauggaaa aauacaacag uuuuaaaucc     3060

aacuga                                                                3066


<210>  4917
<211>  3072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4917
augaaaaucc uuggauuuga uauaggagua gcaagcauug gaugggcauu uguugaaggc       60

gaugaaauca gagauugcgg aguaagaaua uucacgaaag cugaaaaucc aaaaacaggu      120

gaaagucuag cucugcccag aagagaagca cgcggaguaa gacgacggcu cgcuaggagg      180

agaguaaggc uuaacacuau uaagaaaaua auaugcaaag aauuugaccu aagauuaucc      240

gacuauauag cagacgacgg cgaacuaccu aaggcuuauc aaaccaacaa agauacaaaa      300

ucaccauacg aacuaagaua uaaagcacua aacgaacgac uuucaaaaga agaauuagca      360

agaguaauac uccacauagc caaacaucga gguuacggca acaaacacgc uaaagacggg      420

gaugauaaag auagcgguaa aguaaagaaa gcaauaggag aaaauaaaac acuacuagaa      480

caaaaagggu acgaaucagu aggcgaauau ugcuucagag aguucuucaa aucaaucaaa      540

gacaaagaug gggaaacaca caauauacca agccagaaca uacgaaacaa gaaagguaac      600

uacgauagau guguuucgca aaaacaauua gaggaugaac uaaaauuaau uuucgcaaaa      660

caacgaaaac ucggguuuaa uaaagaguuc gaagaugcga uauuagaagu agcuuuuuac      720

caaagaccgu uaaaagauuu ugaugacaaa auaggagacu guauuuucuu ugacaaugaa      780

aaacgagcac caaaagaauc cauaucugca auagaauuug uagcguuaac uagaauaaua      840

aacacacuaa aauaccuauc aaaaacaaca ggagaaguau augaaaaaga aaucauuaac      900

aaaaucuggc aaaagauacu agaaaacggg aaaaugacuu acgcggaauu aagaaaaaua      960

uuagcacuag auucaagcgu guuuuucaag gauaaagacc ucgacuacac cuuagguauu     1020

aaagaagcgg agaaaauaaa auucauagag uucaagaaac uaauaaaauu caagaaagca     1080

cuagggucau ucgauggauu auccagggaa auacucgaca acaucgcaau gcaaauagca     1140

auaacaaaag acaggcaaag auuauuagaa aaacuugaag uguuuccacu aaauaaagaa     1200

cagaaagaaa auuuaaguca acuaaauuuu aguaaauaca uagaucuaag ccuaaaagcc     1260

cuaaaucaga uacuuccauu uaugagagaa ggucgaagau acgaugaagc aaaagaugau     1320

gcgaaauugc aagaaaaagc gaaaaauacu aagaaagaag aguuccuacc accguuaaaa     1380

gaauuugauc uaaacuuaac aaauccagua guagcacgcg cacucgcaga auuuagaaaa     1440

guacuaaacg cccuacuuaa gaaauauggc aaaguucaua aaauacauuu agaguucgca     1500

agagacauga gacuagauuu cgacgaacga gaaaggcuca ucaguauaca aaacgacaac     1560

uucaauaaaa gacaacuugc cgaagauuuu uguaaagaac auggucuaga accaucgaac     1620

gcaaauauuc uaaaaauaaa auuguggcaa gaacaacaau ggcacugcau guacucaggc     1680

gaacucauag ggauaccaga cuuaaaagac ccuacaaaac uacaaguuga ucacauauau     1740

ccauacucga gaucuuuuga ugauaguuau acuaacaaag uacuugucag agcggaaugg     1800

aaccaagaaa aaggcaauca uaccccuuuc gaagcauucg gcggcgauaa aaacaaaugg     1860

gauaaaauug uagcacuagc ugaaaaacua ccaccaucaa agaaacuaaa uagaucagaa     1920

aaaucaaaga aagauaaacu acuagacgaa aaauuuaacg acaaagaacu cggauuuuua     1980

gcacgaaaua uaguagacac agguuacaua gcaagacuug uagcgaaaua uaucgauaug     2040

uaccuugagu ucuuaccacu ugaagaaaau gaaaauaccu cuuuagcccg aggagaaaaa     2100

ggcaguaaga aacauauagg aauaguaaac ggaagacuua caucagaagu aagacauaac     2160

uggggccuug auccuaaaga ucgcgauaau caccuacacc augcuauuga ugccauagua     2220

auugcauaca ugaacgauaa acgaauaaaa gcauuugcag auuucagaag auuaaaggaa     2280

cuaaaaauaa aacgauacgg acgggaaaua acagaagaug aauaccaaga aaagaagaaa     2340

gaacuaucau gcgcaccauu cgacgacuuc agaaaccgaa cccucgaaaa cauugacaaa     2400

auauuuguaa gcagaccacc ccgaaaacgu gcacgaggca cauuacauga agaaaccuuu     2460

uauucuauag augauaagaa acuguuaaaa gcauauggag gaaaagaagg caugcaacga     2520

gcacucaguc uggggaaaau aagacaaauc gguacaaaaa ucguuaaaaa ugggacaaug     2580

guaaggguag acauauucaa agacaaacga ggacgauucu augggguacc gauauauaca     2640

auggacuuug cguuaggagu auugccuaau aaagcagcug ucaguggaaa agacaaaaac     2700

ggauuaauaa aagaaugggc cgaaauggac ucaggauacg aguucugcuu cagccuauuu     2760

aaagaugacu ugauucuagu ccaaagaaaa gaaaugcaag aaccagaauu auguuauuuc     2820

guuagcuucg auaguucaaa ugcacagaua gacguaagaa gucacaauaa ccacuucagu     2880

guacucaacg aaaaucaaaa acuccucuuu aaauccgcaa cacaagcaga aguaauagga     2940

gaauccauag gaauccaaaa ccuaaaagua uuugaaaaau ggcauguauc accguuagga     3000

gaaguacgaa aagcagaauu uaguccacga ggugaagcua aacugaaaac uagcaagaaa     3060

cgaaagaaau ga                                                         3072


<210>  4918
<211>  3072
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4918
augaaaaucu acucauuuga uauaggagua gccucgauag gaugggcuuu cauagaaaac       60

gaaguccuac aggauugcgg aauucguaua uuuacaaaag ccgagaaucc aaaaaacggu      120

gcgucuuuag cauuacccag aagagaagcc agaggaguac gaaaaagauu agcacgaaga      180

agagguagac uuaacacaau uaaacaacug uuauguaaag aauuuggauu acaacucgag      240

gauuaccucu caaacgaugg ugaacuacca aaagcauaca cagcuucgaa agccaauccc      300

uuaaaaucac cauacgaacu ucgaacauua gcuuuaucuc aaaaauuaga cuccaaagac      360

cucguuagag uaauacuaca caucgcaaaa cauaggggau acggaaacaa acacgcuaaa      420

gauuccaagg auaccgaguc gggaaaagua aaaaaggcaa uagaauucaa ucgaaaaaca      480

cuagcagaaa aaggauauag gucagucggg gaauacuugu accgagaauu cuuucaacaa      540

agcagaauca gccaaaacaa cggcgcaaca gaauuuauua acguaagaaa uaaagccgga      600

aacuacgagc auugcguugc ccaagacaug cuaaaagaag aauuagaguc uauccuuaac      660

gcccaaagac aauucggcuu uucacuaaaa caagacuucg aaucaaaauu auuaacuaag      720

auauuugaac agcgaccucu aaaaagcuuc gcagacaagg ucggcaauug cugcuuuaua      780

gcaggcgaaa aacgugcccc aaaagacuca ccaucagcua uagaauucau cgcauuaucu      840

agaacaauaa acacucugau aaacuuaagc aaagaaagcg gugaaauaua uaaccgagcu      900

aucauacucc aaaucguaga aagcacacua aaaaaaggcg agauaucaua uaaagaacuu      960

agagaaauaa uaagacuaga ugaaagguua cguuucaaag acccacgcuu agacuacuca     1020

agagaaauaa aagaggcaga gaaaguuaaa uuccuggaau uuaaacaauu aaaagguuuc     1080

aaaaaggcau uaggcgaauc guuuacaaca cuagcaauuu cacaauuaga ugagauugca     1140

gaacauauag cccuaauaaa agacagaguc aaauugaaug aaaagcuaga aucauacgcc     1200

cucacaaaag agcaaagaga ggcauuauca aauuuaagcu ucucagcaca cauaaaucuu     1260

ucacuucaag cauuaaaaca aauacuacca uucaugcgug gcgacaaugg agaacaaugu     1320

uuagacuaug acgaauccgu ggaaaaagcc ggauuaaaag cacucucuaa ggauacacua     1380

aaaggccaaa ugcugccgcc acuaaaugaa uucgaacccu aucuaucuaa cccgguagua     1440

gcaagagcau uagcugaaua cagaaaagua uuaaauguau uacuuaaaca auacgguuca     1500

ccccauaaaa uucacauaga auaugcuaga gaagcaaaac uaagcucaac ugaaagacaa     1560

aaauaugaaa aagaacaaaa agauaauuau gcugcuaacc aggcagcaca uaaaaaaugu     1620

caagaauuag gcuuagaacc uucuagcaca aauuuauuaa aacuaaaauu auggcaagaa     1680

cagggaggac ugugccuaua uucaggagca aaaauaacga uaucauaccu ccaagaucca     1740

acagcacugc aaguagacca uaucuacccu uauucccgcu cauuugacga cagcuauaug     1800

aacaaauguu ugguacuaac caaagcuaac caggaaaaag gaaaucggac uccauuugaa     1860

gccuucggaa cagacagugc aaaauggggu guaauaacca cauuagccca aaaacuaccg     1920

cauaaaaaaa gaagaagaau auuaaauaca gcauucaaug acaaagaagc aggauuuaaa     1980

cuuagaaacu uaaacgauac aagcuacaua gcgagacuug uagcugacua caccgaaacg     2040

uaccuggaau uucuaccucu uguagaauca gagaacacaa cuuugucaaa aggccaaaaa     2100

gguucaaaaa aacacauagc aauaguaaac ggaguacuaa caaguacuau gagguacuac     2160

cuaggcuucg cgaguaagga cagagauaac caccuacacc augcccuuga ugccguuauc     2220

auuggauuca ugaaugauag cgucauaaaa gccuuuagcg acuucaaaaa aacacaagag     2280

acaagcaaag cugccuauua cgcacauacc cuaucuaaac aagaauacaa aaaacaaaga     2340

gcauucauac aacuacccag uggagaaaac uuccgaguau caguauuaga aaaagucgaa     2400

aguguauucg uaucaaaacc ucccagaaaa aggguacgag gagcccuaca cgaagccacu     2460

uuuuauucac caaacgaucc caaacuccua aaaaacuacg gagguaccaa aggaguagaa     2520

cgagcuuuag cuuuagggaa aaugagaaaa guuggcgcaa aaauaguauc aaauggagca     2580

augguaagag uagauauuuu cagacacaaa agcaguggaa aauuuuaugg cguaccagug     2640

uacacuaugg acuuugcacu cggcauauua ccaaacaaag ccgucgucgu aggcaaagac     2700

caaaaugggg ucauaaaaga cuggcuagaa auggacaguu cauacgaauu uauuuucuca     2760

cuauacaaag acgacguaau acuaguccaa aaaaaagaaa ugcaacggcc agaauuaugu     2820

uacuucguau cuuuugacac aucaggugcc ucaauaaaag uagcaaaaca ugauaauaau     2880

uucucuaccu uaacagaaaa ucaaaaauua cuauuuagua augcuacaaa agaagaggua     2940

guagguaaaa gcauaggcau ucaaaaccua aaaguguucg aaaaauggca aguaagcgua     3000

uuaggagaag uuaaaagaac acaaaguuau ccgagagaaa auaucagcuu agcaagcaaa     3060

gcuaaaaaau ga                                                         3072


<210>  4919
<211>  3075
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4919
augauaagga cacugggaau agauaucgga auagcaucaa uaggaugggc aguaauagaa       60

ggagaauaua cagauaaagg auuagaaaau aaagaaauag uagcaucugg gguaagaguc      120

uuuacaaaag ccgaaaaccc caaaaacaaa gaaucauuag cucuaccgag aacauuagcc      180

agaucagccc guagaagaaa cgcaagaaaa aaaggcagaa uccaacaagu aaaacacuac      240

cuaaguaaag cauuaggacu agaccuagaa uguuucguuc aaggggaaaa acuagcaaca      300

cuauuucaga ccuccaaaga cuuccucagu ccaugggaau uaagagaaag ggcacuauac      360

cguguucucg auaaggaaga acuagcaagg guuauccuac auauagcuaa acgaagaggu      420

uaugaugaua uaacuuacgg aguagaagau aacgauucag guaagauaaa aaaggcaauc      480

gccgaaaaca gcaaaagaau aaaagaagag caaugcaaaa ccauuggaga aaugauguac      540

aaauuauauu uccaaaagag cuuaaacguc aggaacaaaa aagaaaguua uaaucgaugc      600

guaggaagaa gcgaacuaag agaggaauua aaaaccauuu uccaaauaca acaagaacua      660

aaaucaccau gggucaacga agaauuaaua uauaaacuau uagguaaucc agaugcacaa      720

aguaaacaag agagagaagg auuaauauuu uaucagagac cucuaaaagg auucggagac      780

aaaauuggaa aaugcucaca uauaaaaaaa ggagagaacu caccuuaccg ggcauguaaa      840

caugcuccau cagcugaaga auucgucgca cuaacaaaau ccauaaauuu ccuaaaaaau      900

uuaacaaaca gacacgggcu augcuuuagc caagaagaua ugugugucua ccuaggaaaa      960

auacuccaag aagcucaaaa aaaugaaaaa ggacucacuu auagcaaacu uaaacuauua     1020

cucgauuuac cgagcgacuu cgaauucuua gguuuagauu auucagguaa aaauccagaa     1080

aaagccguuu ucuuaagucu acccaguaca uucaaauuaa acaaaauaac acaagacaga     1140

aagacacaag auaaaaucgc aaauauacua ggagcuaaca aagacuggga agcaauauua     1200

aaagaauuag agucacuaca acuaucaaaa gaacaaauac agacaauaaa agaugcaaaa     1260

cuaaauuuuu cuaaacauau caaucucagc uuagaagcuu uauaccacuu acugccccug     1320

augagagaag guaaaagaua ugacgaagga guagaaauac uacaagaaag aggcaucuuc     1380

agcaaaccuc aaccaaaaaa ccgacaacua cuaccucccu uaagcgaacu agcaaaagaa     1440

gagucauacu uugacauccc aaaccccguc cuaagacgag cuuuaaguga auuuagaaaa     1500

gucguuaacg cccuacugga gaaauacggc ggauuccauu acuuucauau agaauuaaca     1560

agggacgugu gcaaagcuaa aagugccagg augcaacucg aaaaaauaaa caaaaaaaac     1620

aaauccgaga augacgcggc aucacaacuc cuagagguac uaggauuacc caauacauac     1680

aauaaccgac ucaaaugcaa acuauggaaa caacaagaag aauauuguuu auacaguggc     1740

gaaaaaauua caauagacca cuugaaagau caacgagcuc uacaaaucga ccaugcauuu     1800

ccacuuucaa gaagucugga ugauucacaa uccaacaaag uucuaugccu aacgucaucc     1860

aaucaagaaa aaaguaacaa aacucccuac gaauggcuag gauccgacga gaaaaaaugg     1920

gacauguacg uaggaagggu auacagcuca aacuuuaguc ccagcaaaaa acguaaauua     1980

acacaaaaaa acuucaaaga acgaaacgaa gaagacuucu uagcaagaaa ccuaguagac     2040

acagguuaca uuggaagagu cacaaaagaa uauaucaaac auucccuauc auucuuaccu     2100

cuacccgaug guaaaaaaga acacauuaga auaaucucag gauccaugac aaguacaaug     2160

cguagcuucu gggggguaca agaaaaaaac agagaucacc accuacauca cgcacaggac     2220

gcgauaauua uagcauguau agaacccagc augauacaaa aauacacaac auaucuaaaa     2280

gacaaggaaa cacauagacu aaaaagucac caaaaagcac aaauccuucg agaaggugac     2340

cauaaauuaa gccuacgcug gcccaugagc aauuucaaag auaaaauuca agaaucaaua     2400

caaaacauaa uaccaaguca ccacguaucc cacaaaguca ccggagaauu acaccaagaa     2460

acaguaagaa ccaaagaauu cuacuaucaa gcguucggag gagaagaagg aguuaaaaaa     2520

gcauuaaaau ucggaaaaau ucgagagaua aaccaaggaa ucguagacaa cggugcaaug     2580

guuagaguag auaucuucaa aucaaaagac aaagguaagu uuuacgcugu ucccauuuau     2640

acauacgauu ucgcaauagg aaaauuaccc aacaaagcaa uuguccaagg aaaaaaaaau     2700

gguauaauaa aggacugguu agaaauggac gagaacuacg aauucuguuu cagccucuuc     2760

aaaaacgauu guauaaaaau acaaacaaaa gaaaugcaag aggcugucuu agcuaucuac     2820

aaaucuacaa acucugccaa agcaacaauc gaacuagaac accuaagcaa auacgcuuua     2880

aaaaacgaag acgaagaaaa aauguucaca gauacagaca aggaaaaaaa uaaaacaaug     2940

acaagagaaa guugcggaau acaaggauug aaaguauuuc aaaagguuaa auuaagcguc     3000

cugggugagg uauuagaaca caaaccucgu aacagacaaa acauagcuuu aaaaacgaca     3060

ccuaagcacg uuuga                                                      3075


<210>  4920
<211>  3078
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4920
augggaggau auaugccaca auuaaugcga uaucgccuag cauuagacau uggaucaaca       60

ucaauuggau gggcaaugau aagauuauca augcaaaaug agccaauagc uauaauaaga      120

augggcguua ggauauucuc agauggaaga aaccccaaag auggaucaag uuuagcugua      180

acccgaagag uugccagaca aaugcguaga cgaagagaua gauuacuaaa aagaaaaaca      240

cguuuacuaa augcacuggu acaaaagaaa uuuuucccug aagaugcaga ugccagaaag      300

gcuuuaguag uuuugaaucc cuaugaauua cgaaaagaag gccuauauag gcaacuauca      360

ggcccagagu uuggcagagc acucuuucau auaucccaaa gaagggguuu ucuauccaac      420

agaaaaacag acaagaaaga caacgacagc ucagucuuaa agaaagcuau aguugaccuc      480

agacaacaac ucgaaguaga gaauugucaa acuauagggg aaugguuguu uaaacaacaa      540

caaaguggca aaucagucag agcaaggcuu agaggaaaaa cgcagaaaga caaagcauau      600

gacuuuuaua uagauagagc aaugauagca cacgaauuug acacuuuaug gguaucacaa      660

gcuaaauaug auccuuuacu guuuaacgaa gaugccagag caauacuaag agacauucua      720

cuauaccaaa gaccgcuaaa accagugaaa cccgguaggu guacucuaau accagacgaa      780

ucucgugcuc cauuagcacu acccuuaaca caacaagcaa gaauauacca agaacuaaau      840

caucuucgca uuaccacacc agcgcuagga gcacaggcuu uaacaguugc acaaagaaau      900

acgauagcag cccuucugga aaagagcgac guaacauuug uaaaaauagc caaagcacuc      960

ggacuuacaa guacaacgga guucaauuua caagacauua aaagagauag guuaaaaggu     1020

aacgcuacag caauuaucuu agcuagaaac caauacuucg gacgacgaug gcaugauuuc     1080

aaugcugauc uacaaaaccu aauuguagau aaauuaguua augaaggcuc agaaacagca     1140

uuaauagaau gguuaaaagc aaauacagac guugacgaau cagcagcuga aaggguauca     1200

gguguuacac ucccagaagg uuaugggucc uuaaguguag cagccuuaag aagauuauua     1260

ccagcauuaa aagaggacgu aguaguauac agcgaagcag ucaaaagagc aggauuugau     1320

ucacacucug cuuuaaguuu aauugaacaa acaggagagu uauaucaaga acuaccuuac     1380

uauggagaag uacuacaaag acauguaggg uucggaucag gugauccaaa ugauagucca     1440

gaacgucgau uuggaaaaau ugccaaucca acaguacaua uaggguuaaa ugaacuaaga     1500

aaaauaauua augcguuaau aaaaagauau ggaaauccag cagagguagu uguagaguua     1560

gcaagggauu uaaaacuaag ucgagagaaa agaaaagaaa uacaagaaga acaaaaacaa     1620

cgacaagauc gaaacgaaaa gagaauaaaa gaugcuugcg aaauucuagg uauagauccu     1680

cuagugauug accguggcaa aagaagagaa auuucucaga aaaugcaacu augggaugaa     1740

uuaaacccaa aagacgcagc aaauagacgu ugcccauaua caggggauca aauauccaua     1800

gaaaaauuac uuagugcaga agucgaaaua gaacacauau uaccauucuc caaaacacuu     1860

gaugacaguu uaaauaauaa aacuguagca augaggagag caaauagaga caaaggaaau     1920

cgaacaccau augaagcauu cggagaaagu cuuaacccag auuaugauua ugacgcaauc     1980

cuacaacgag caucucuuau gcccagagaa aaacgaaaaa gauucgcuag cgaugguuac     2040

caacaauggu uacgagauga uaaagacuuu cuaagcagag cuuuaaauga cacggcguau     2100

cugucgcgga uagcaaaaga auauuuaaca uuaauaugcc cuccuaauuc aguacgagcc     2160

auuccaggac gaaugacugc acaauuacga gcuauguuug gucuaaauag auuauuaucc     2220

gaaggcggaa ucaaaaauag agaagaucac agacaucacg caauagaugc agcgguaaua     2280

ggaguaaccg aucaaggacu auuacaaaaa uuuuccagag caucagcacg aacacgugaa     2340

uuacaauuaa guagacuugu ugaugacaug ccuuuaccau gggacacaua cagagaacac     2400

gucacaagag cauuagagca uuuaguaguc agucacaaac cagaucacgg auaucaaggc     2460

aaaaugcaug aagauacugc uuggggauua agggacaacg gacaaguaac aagacguguu     2520

cgcucagaag auggaggacc aagacaacgu cuccuagaaa auaaaaacgu cguuaagaua     2580

aauucaacac gcgacuuuag uagacaugga auagaugaac aaggccaagu uaacgcauau     2640

aagggguacg uuggaggaag uaacuacugc auagaaaucu ggauagauga aaaaggaaaa     2700

uggaacucag acguaguauc aacuuuucaa gcauaucaga uagucagaga auuaggugaa     2760

aaggccgcau ggcaaaaauu acguaaucca uuauuauccc ugagugcaaa accacucguu     2820

augagauuaa ucauagauga uguagugaaa augcaaauag cuggacaaaa uaaaauaaug     2880

agaguuguga aaauagccca aaauggacaa auauuuuucg cggaacauaa ugaagcuaac     2940

guagaugcuc gagauaagga caaaaacaau ccauucaaau auauaucaaa aacggcgggu     3000

ucgcuccaaa aagcaggcgg aauacauaug gcaauaucgc ccaucgguga cgcaagaccg     3060

guacgauaug gaagauga                                                   3078


<210>  4921
<211>  3084
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4921
augaaauaua cauuaggacu agacguaggc acuaauucaa uaggcugggc cguauuagaa       60

cuaaacacua acggagaacc acaaagaaua gaaaaauuag gaaguagaau auucacugac      120

ggaagacacc caaaagaugg aaccacacua gcuagcaaac gaaggcaaaa acgccacgaa      180

cgcagaagaa gggacagauu gcaucaaaga aagaagcuag uacucaauca auuaguaagc      240

augggucuau ucccacaauc agaacaagaa caacaagauc uaaaaagccu agacguacua      300

acauuacgag cgaacggcgu uucaaaacaa cuaucugcuu augaacuagg aaggguauuc      360

uacuauuuaa acuuaaaaag aggauucaaa aguaacagga aaggcggauc agaagaagac      420

aaagcuaaca aaauauccga aagaauuaag aaacuacaag aacaacuaaa agaaaaugau      480

uucaaaacag uaggccaaua uuuacacgac cgguauauaa aaggacuauc cacaaaagca      540

acaauagaaa acgacuuuca ccuauuaaga caacuuauag aagacgaguu cgauuucaua      600

guagaaaccc aaucaaaauu cuuccccgaa aucuccgaug aacaauggaa uacacuccgg      660

aacagaaugu uccaucaaag acccuuaaaa ccaguggaaa caggcacaug uagucuauuc      720

gaagacaaga aacgaacaua caaauacaug ccaaguuuug aagaauauag auuccuaacu      780

gaacucuuca accuguccua ucaagacgaa aacuuucacu cacauucacu aucgucagaa      840

caaauuuuca aagucuucca cgaacuuaaa aacacacgag aagcaacaua uacuaaaaua      900

aagaaauuau uaaaacuccc cagcgacuua aguuucucua uagaaagauc aaaaucaaaa      960

gauaaaauaa aaaucucaac uacaaaccac uucuucucca aaucauuacu auucgcagaa     1020

gaaugggcca gccuagaucu aaaacuaaaa gacgaaguag cugaaauaua uuucuccgac     1080

augucgguag aagagauuaa gaaaggauua cuccaacuaa aaaucgauuc cgaaaucaua     1140

gaaaaccucc uagaagaaaa agucccaaac guacaagaag cgaccugcag cuacucaaaa     1200

gaagcacuac agaaaauagu aucauuaauc cucgaagaag aacgucaccc cacacuaaua     1260

gucgacgacc uauucaaaaa cgaaaaagaa gaaacgucac ucauagaccg acuagacuac     1320

uacggagcau caauuccaga cucaaugcaa ccuauaccac aacauaucaa agacaauaac     1380

uuaacauuaa augaagauga aaaacgcuac gggaaaaucg cuaaccccac aguacaugcc     1440

gcucuaaauc aaauucggca agucguaaau gaccucauag aacaauacgg aaaaccaaca     1500

aacauacaca ucgaguucgc aagagauuug aaaaagucua aagaagaacg agacuucgau     1560

aaccgaaaga aaaagaaaaa cgaagaacua aacgaacaag uaagagacuu cauagaaaaa     1620

cacaaucaaa aaccuucugc cuuuaauuuc gaacgaguua agcuaugguu cgaacuagac     1680

gcaaugaaua aucaaguaug cgcauacuca ggaaaaacaa ucucugcacg caugguacuc     1740

ucagacgaag uccaaguaga ccacaucuua ccauuuagca gaacauuaga cgauuccuua     1800

gcgaauaagg uguuaguauu agcuucggaa aauguaaaga aacguaauag aaccccauac     1860

gaagccuucg gucaagauac agaaaaaugg gcaucaauuc aagaauuagc caaaagacua     1920

ccauaucaca aacaauggag auuugcagaa gacgcuauua agaaauuuga agaaaaagau     1980

caauuccuac aaagacaucu caaugauaca agauauauau ccaaaauagc aaagaaauau     2040

uuaggcucua ucaccgaucc auauaaaaua gucgcaucac gaggccaaau gacauccaua     2100

auaaggggua aauuaggacu aaaucaauuu auccaaaaac ccgauggaac caaaaauaga     2160

gaagaucaua gacaucaugc uaucgacgcc uuaacaguag cgcuaacauc ccgaaguuac     2220

cuaaaacgaa ucagugacgc aucagcaaga aaucaagacc ccaacagaau acccauaccc     2280

gaaccaugga aaggcuuuau aaaagacgca gacgucaaau ucaaugaaau aauaguuucc     2340

cacaaagucg accaugguaa aaacggacca uucauggaag aaacauguuu ugguuugauc     2400

aaaaacccca auaaauauga agaagaaaac gacuucaaac uaaucacaac aaaaucauua     2460

gacuccguca aagacccauc uacgauuaga agucagagcu ugcaaaacuu agcagaaaaa     2520

ucuggaguaa ucuccuuggc caaaucaggg guaaagaaac uacgugucua ugacguaacc     2580

aaagaaaauc cugcugacau cgguuuaaua gaaaguggac uggccaaaau aacucacgga     2640

aaagauaaac aacacguuaa acauuaccaa aaaggagaca uaaauuacuu agcaaucugg     2700

ugucuaccgc aaaauauaac cuuaaaauca gacaaccaca agaaacgcaa aacagauuau     2760

auauuuacag caauaaagac auuugaccua aacaguaaaa auccaaauga ucuaagaccc     2820

cauccugcag caaaauuagu aacaaaaguc uauaaaggcg acacaguagc uuuagaagua     2880

aauaaccaau uaaacuacua cauuguaaaa ucaauacgag cagcaaauaa aaagcuauau     2940

uuccuaaaaa ucaaucaauc uuccgaauua gccaacgaaa cacaauucau acccgcauac     3000

accaagcuag uagacuauaa auuccgaaaa gucucaguau caccuaccgg cguaauaaca     3060

gauaauggac cacuacuaaa auga                                            3084


<210>  4922
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4922
augaggagcc uuagauauag auuagcauua gacuugggau caacgagucu aggaugggca       60

cuguuccguu uagaugcaug uaauagacca acagcaguaa uaaaagcugg agugagaaua      120

uuuucagaug gaaggaaucc gaaggauggu uccagccuag ccguuacaag gagagcggcu      180

agagcaauga gaagaaggag ggacaggcua uuaaaaagaa aaacaagaau gcaagcaaag      240

uugguggaac acggcuucuu uccggcagac gcaggaaaac gaaaagcucu agaacaacuu      300

aauccuuacg cauuaagagc aaaagggcuc caagaagcau uauugccagg cgaauuugca      360

cgagcauuau uucacauuaa ccaaagacga ggauucaaaa guaauaggaa aacggauaag      420

aaagacaacg acucaggugu auuaaagaaa gcuauuggac aacugagaca gcaaauggcu      480

gagcaaggaa guagaacagu gggagaauac uuauggacac gauuacaaca aggucaaggc      540

guaagagcaa gguaccguga gaaaccauau acuacugaag aaggaaagaa aagaauagau      600

aaaucuuaug aucuauauau ugauagagca augauugagc aggaguucga cgcuuuaugg      660

gcagcacaag cugcuuuuaa cccuaccuua uuccaugaag cugcucgagc agauuuaaaa      720

gauacacucu uacaccaaag gcccuuaaga ccaguaaaac caggccgaug uacucuuuug      780

cccgaagaag aaagggcacc cuuagcacuu cccagcaccc aaagauuccg cauucaucaa      840

gaaguaaauc auuuaagauu auuagaugaa aaucuaagag aaguugcccu aacacuggcc      900

caacgagaug caguaguaac agcucuagaa accaaagcua aauuaaguuu cgaacaaauu      960

agaaaacuac uaaaacuuuc aggcucugua caguucaauu uagaagacgc aaaacgaacg     1020

gaacuaaaag gcaacgcaac aucagccgca uuggcaagaa aagaacuauu uggugcagcg     1080

uggucaggau uugaugaagc gcuacaagac gaaauaguau ggcaacuugu aacagaagaa     1140

ggagaaggag cauugauagc augguuacag acacacacug guguagacga agcaagagca     1200

caagcgaucg uugaugucag ccuuccagaa ggauacggua aucuaucucg gaaagcauua     1260

gcgagaauag uaccagcuuu aagagcagcc guaaucacau augauaaagc uguacaggcu     1320

gcaggauucg accaccauuc acaacuuggc uucgaguacg acgcauccga aguagaagac     1380

uuaguucacc cagaaacagg ggagauuaga ucuguauuua aacaacuccc auacuauggu     1440

aaagcacuac aaagacaugu agcuuuugga agugggaaac cagaggaucc ggaugagaaa     1500

agauacggga agauagcaaa uccaaccguu cauauaggau uaaaucaagu acgaauggug     1560

guaaaugcgu uaauacggag auacggaaga ccuacagaag ucguaauaga auuagcaagg     1620

gaccuuaagc aaagcagaga acaaaagguc gaagcucaaa gaagacaggc agauaaucaa     1680

agaagaaaug caagaauacg acgaagcaua gcagaaguau uaggcauagg cgaagaaagg     1740

guaagagguu cugauauuca aaaauggaua uguugggaag aauuaaguuu cgacgcagca     1800

gaucgaagau guccguauag uggaguacaa auaucagcag caauguuguu aucagaugaa     1860

guagaaguag aacauauacu accguuuuca aaaacccuug acgauagccu aaauaauaga     1920

acaguagcaa ugcgacaagc aaacagaauu aaaagaaaca gaaccccaug ggaugcacgc     1980

gcugaauuug aagcucaagg cuggucauac gaagauauau uacaacgggc agaaaggaug     2040

ccccuaagaa aaagauacag auucgcaccg gauggauaug aaagaugguu aggagacgac     2100

aaagauuuuc ucgcccgagc ucuaaacgau acuagauauc uaucuagagu ugcagcagaa     2160

uaucuaagau uaguaugucc ugguacccgu guuaucccag ggcaacuaac ggcauugcua     2220

agaggaaaau uuggguuaaa ugauguauua ggauuagaug gagagaaaaa ucguaacgau     2280

cauagacacc augcaguaga ugcauguguc auagguguaa cagaucaagg acuuaugcaa     2340

agauucgcaa cugccucagc acaggcaaga ggugaugggu uaaccagauu agucgacgga     2400

augccaaugc cuuggccaac cuauagagau cauguugaaa gagcagucag acauaucugg     2460

guuucacaua gaccagacca cggauucgaa ggggcaauga uggaagaaac uucauaugga     2520

auacgaaagg auggauccau uaaacaaaga agaaaagcag auggaucagc aggucgugaa     2580

auaaguaauu uaauacgaau acacgaagca acccagccau uacgacaugg aguauccgca     2640

gauggccaac cauuagcaua caaaggauau guaggaggga guaauuacug cauugaaauu     2700

acagucaaug auaaaggaaa augggaagga gaaguaauau caacuuuuag ggcauaugga     2760

guaguaaggg cgggaggaau gggacggcua cgaaaccccc augaagguca aaauggaaga     2820

aaguuaauaa ugagauuggu aaucggggau ucuguacgcu uagaaguuga uggagcagaa     2880

cguacaauga gaauuguuaa aaucucaggg ucaaaugguc agauauuuau ggccccaauu     2940

cacgaagcaa auguagaugc uagaaauacc gacaaacaag acgcuuucac auauacaagu     3000

aaauacgcug ggagcuuaca gaaagcaaag acuaggagag ugacaaucuc gccgauaggc     3060

gaagucagag aucccggcuu caaagguuga                                      3090


<210>  4923
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4923
augaaauacc gacuugccuu agauuuagga aguacgucac uaggaugggc uauaguccaa       60

cucaaugcca aagaugaacc uugugcgauu guacgagcug gaguuagaau uuuuccugau      120

ggaagaaacc caaaagaugg uuccaguuug gcagucacac gaagaaaugc aagggcuaug      180

cgaagaaggc gagacagacu ucugaaaaga aaagcaagaa ugcaaagacu auugauagaa      240

caugaucucu uuccagcaga ugaagcagca agaaaggcgu uagagacccu gaauccauau      300

gagcuaagag cuaaaggauu agaugcagca cuuacaccag cugaauuugc uagagcucua      360

uuucauuuaa aucaaaggag aggauucaaa aguaacagac gcaccgauag aaaagacaau      420

gacucuggag ccuuaaaagc ggccauaggg ggucuacgag cucaacuaga uacagcggga      480

gcggacggca gagcaagaac cguaggugaa cuauuaugga gaagaaugag cgauccaaau      540

gucuuaacgg uccgagcaag auauagggaa caaagaauaa ccaaagauga cggaaagaca      600

agaguggaca aaucauacga uuuauaugua gauagggcua uggucgaagc agaauuugau      660

gcauuauggg caaaacaagc agaacuaagc ccuacacaau uuggggaagc agcucgagca      720

accuuaaaag acuguuuauu acaucaacgu aauuuaagac cgguuagacc aggaaggugu      780

acguuagaac cagacgaacc acgggcuccu uuagcauuac caucccaaca aagauuuaga      840

auacuucaag aaguaaauca ucuaagauua uuaggaggca gacuacuaga agagaguuua      900

ucaguagagc aaagagacaa auuaguagcu gcuuuggaag auggcucaaa aucauuuaaa      960

acggccauac caaaauuacu uggauuaccg gggacagucc aauuuaaucu agcagacgca     1020

aaaagaacag aacuuaaggg uaaugcaacg aguaugcuac ucggcaagaa agacuuuuuc     1080

gguccagcgu ggcauggauu uagcgaagca cgacaagaug aaauugugau gagaauacaa     1140

gaagaagaau cggaagccag acucguagau uggcuagcaa caucaaccgg agucgaugaa     1200

gcucaugcgc uuaaaauagc uaaugcagcg cuaccagaag gguauggaag ccuaagugca     1260

aaagcacugg caaagaucuu accagcgaug agagcagaag uaaucacgua cgauaaagcu     1320

guuuuagcag caggauauga ucaucauuca cacauaagcc augcagcuuc aacgggugaa     1380

auauuaccag aauuaccaua cuauggagaa uacuuacaga gacacguagg auucggaacu     1440

ggggaauuaa acgauacccc ugaaaagagg uuuggacgga ucgcaaaucc aacaguccau     1500

auaggacuua aucaaguuag aguaguaguu aaugcauuga uaaaaagaua uggacaucca     1560

acagagguca uaguagaagu ugcaagagac cuuaaacaaa gccaagauca aaggagagag     1620

acacaagaac aacaagcuaa aaaucagaaa agaaaugaaa gauuaagaaa agaugcagcu     1680

gcgauacuag gaauagcaga cgagagagua cgauuagccg acauacaaaa auuaauauua     1740

ugggaagaau ugaguuuuga cauagcagac cguagauguc cauauagugg aguucaacua     1800

agcgcagcca ugguauuauc agaacaagua gaaauagaac acauuuugcc uuuuagcaug     1860

acuuuggaug auagcuugaa caacaaaacc gucgcuauga gguuagugaa cagauuaaag     1920

gggaauagaa caccauggga ugcuagggaa gcauuugccg gccaaggaug ggauuaugac     1980

gggauauuaa ugagagcgga acguaugcca cgagcgaaaa gacaaagauu ugcaccugac     2040

gggcuugaaa aaugguugaa agaagacaaa gauuucuugg ccagggcauu aaaugacacu     2100

agacaccuaa gcagaguagc gagggaauau cucagccugg uuuguccugc aucgacaaga     2160

guaauacccg gacaaaugac agcaaugcua agagcuaaau ucggucuaaa ugacauacua     2220

ggacuuaaug gcgaaaagaa cagaaaugau cauagacauc augcaguaga ugcaugugua     2280

guagcgguua cggaccaggg auuauuacag agguuugccc aagccucagc agacgcacga     2340

ggcaaagguu uagaucgucu cguagaaacc augccagauc caugggacag cuauagagaa     2400

cacgugcaac gagcuguuaa uagcauaugg guuagccaca agccggacca uggauuugaa     2460

ggagcuaugc acgaagauac aacauaucgu ccaccacacu uagacccgaa aaacaugugg     2520

agaacgagag ggauaggagg uggaaaacca aacgaaaaag aagccgacag caaggcaaug     2580

guaggauuua caggaccuga acaucuacag agacaucaua gagauaaagc cggucaacaa     2640

cugccauaua aaggcuaugu agcaucaccc aaauuuugua uggauauagu ucggaaugac     2700

aaaggaagau gggaaggaga aguuguguca acauuugaag cguauagguu aguaagugaa     2760

uuuggacgag augcagcucu agcccauaua caaucaaaaa guagaagcau aucaggcaag     2820

ccacucguaa ugagacugca aggaggagau augguacgau uugaugacga ugaagggcga     2880

ccaguaguuc uuaggguugu caauaugacu gguaauggac aaauauucuu ugcuccacau     2940

aaugaagcaa acggagaugc gagaaaccga gauaaacaag aucaauuugu uuauuuauca     3000

aaauacgcag ggagcuuaca gaaagcaaga ggccgcagag uaaccgucag cccagcagga     3060

gaauuaagag acccuggauu uaaagaauga                                      3090


<210>  4924
<211>  3090
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4924
augcgaaguu uacguuacag auuagcacuu gauuuaggaa gcacaagucu agguugggca       60

cuauucagac uggaugcuug uaacagaccu acagcaguga uaaaggcagg aguacgaauc      120

uucagugaug gaaggaaucc aaaagauggu ucauccuuag caguaacaag gcgugcugcc      180

agagcaaugc gaagaagaag agauagguua uuaaaaagaa aaacaagaau gcaagcuaaa      240

cuggucgaac augguuucuu uccugccgau gcaggaaaaa gaaaagcacu agaacaauua      300

aauccauacg cccuaagagc gaaaggauua caagaagccc uucuacccgg agaauuugca      360

agagcucugu uccacauaaa ucaaagacgu ggauuuaaga guaauagaaa gaccgauaaa      420

aaggauaaug acucaggugc auuaaagaaa gcaauaggac aacuaaggca acaaauggca      480

gaacaaggau caagaacagu aggagaauau cuuuggacaa gguuacaaca aggucaagga      540

guaagagcuc gauauagaga gaaacccuau acaaccgaag aagguaagaa aaggauagau      600

aaaaguuaug acuuauacau cgacagagcu augauugaac aagaauuuga cgcccuaugg      660

gcggcccaag cagcauuuaa cccuacguua uuucacgaag ccgcacgggc ggauuuaaaa      720

gauacccuac uccaccaaag gccauugaga ccaguaaaac caggacgaug uacuuuacua      780

ccugaagagg aacgagcccc auuagcauua ccaucuacac agagauucag gauacaucaa      840

gaagucaauc aucuacgauu acuugaugaa aaucuaaggg aaguagcgcu uacacuugcu      900

caaagagaug cgguaguaac agcguuagag acaaaagcga aauuaucauu ugaacaaaua      960

cguaaauuau uaaaauuauc aggaucggua caguuuaauu uagaggaugc caaaagaaca     1020

gaguuaaaag guaaugcuac cagugcugcc uuagcaagga aagaauuguu uggugcggcu     1080

uggucuggau uugaugaggc uuugcaagac gaaauuguuu ggcaauuagu aacugaagaa     1140

ggggaaggcg cgcuaauugc augguuacaa acccauacag guguagauga ggcacgagca     1200

caggcaauag uagauguauc gcuuccugaa ggauacggua aucuaucaag gaaagcauua     1260

gccagaauag uaccugcacu acgagcagca guaauaacau augauaaagc agugcaagca     1320

gcugguuuug aucaucacuc acaauugggc uucgaauacg acgcaucaga gguagaagac     1380

cuaguacauc cagaaacagg ugaaauaaga ucaguauuua aacaacuucc auauuacgga     1440

aaagcccuuc aaaggcacgu ugcuuuuggc ucagguaaac cagaagaccc agacgagaaa     1500

agauauggaa agauagcaaa uccuacagug cauauagguu uaaaccaagu aaggaugguc     1560

gucaaugcac ugauuaggag auauggaaga ccaacugaag uaguaauaga acucgcacga     1620

gauuuaaaac aaaguaggga acaaaaagua gaggcacaaa ggcgacaagc ugauaaucaa     1680

aggagaaaug cacgcauaag aagaucuaua gcagaaguau ugggaauagg agaagaaaga     1740

gugcgaggaa gugacauaca aaaauggauu ugcugggaag aacucagcuu ugacgcagcu     1800

gauaggcgau guccauacuc cggggugcaa auaagugcug caauguuauu aucagaugaa     1860

guagaagugg aacauauacu accauucagc aaaacauugg augauaguuu aaauaaucga     1920

acgguagcua ugaggcaagc aaacagaaua aagcgaaauc gaacgccaug ggaugcgcga     1980

gcggaauuug aagcacaagg auggucguac gaagauauau uacaaagagc cgaacgaaug     2040

ccccucagga aacgauaucg auucgcaccg gaugguuaug aaagaugguu gggugacgau     2100

aaagacuuuc uagccagagc uuuaaaugac acuagguacu uaucaagagu agcagcggaa     2160

uacuuacgau uaguaugccc agguacgagg guaauacccg gacaguuaac ggcauugcuc     2220

aggggaaagu uuggcuuaaa cgauguauua ggcuuagacg gggagaaaaa uagaaaugac     2280

caucgacacc acgcaguaga cgcuuguguu auugguguaa cagaucaagg uuuaaugcaa     2340

agguuugcua cagcaagugc ucaagcacga ggagaugguc uaacuagauu gguagaugga     2400

augccuaugc cuuggccgac uuacagagac cauguagaaa gggcuguacg ucauauaugg     2460

guaagucauc gaccagacca uggauucgaa ggagcuauga uggaagaaac cucauaugga     2520

auaagaaaag auggauccau uaaacaaaga agaaaagcag auggcucagc uggcagggaa     2580

auaaguaauc uaauaaggau ucaugaagca acacaaccac uaagacaugg ugucucugcu     2640

gauggacaac cauuagcaua uaaaggauac guaggagguu ccaauuauug cauagaaauu     2700

accguaaaug auaagggcaa augggaggga gaagugauuu caacauuuag agcauaugga     2760

auaguaagag cgggagguau gggacgauua cguaaucccc acgaggguca gaaugggcgc     2820

aaauugauaa ugagauuagu uauaggagac ucgguaagac uagaaguaga uggagcagaa     2880

agaacaaugc gaauaguaaa aauaagcgga agcaauggac agauauuuau ggcaccaaua     2940

cacgaagcaa auguagacgc aagaaauaca gauaaacaag augcguuuac auauacaucu     3000

aaauaugcag gaagucuaca gaaagcgaaa acgagaagag ucacgauauc accuauaggu     3060

gaaguaagag auccaggcuu uaaaggauga                                      3090


<210>  4925
<211>  3096
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4925
augccagcaa ucuacagguu ggcauuagac cugggaagca cuagccuugg augggcggua       60

cucagacuag aaccggcagc gcuagaaccg auagcaauaa ucaaagcggg cgugagaauu      120

uuuagcgaug guagaaaucc caaagauggc gcaaguuuag cuguaacacg gagagcugca      180

agggcuauga gacguagaag agauagauua cucaaaagga aagcaagaau gcaagacaaa      240

uugguaaaac auggguucuu uccaucagau auggaugaaa gaaaagcacu ugaaagacua      300

gauccauaug cuuuaagggc uaaaggguua cgugaagcau uaacuccagg ugaauuugca      360

cgugcauugu uucacauuaa ucaacgaaga ggauuuaaau cuaauagaaa aacagauaag      420

aaagacaaug acucaggugc gcuaaagaaa gcuauaucgc auuuacggca acaaauggaa      480

gcaggaggac acagaaccgu aggugaucac cuauggucaa gacugcaaca aggauugggc      540

guaagagcuc guuaucgaga aacaccauuu acuaaugaug aagguaagaa aagaauugau      600

aaaucauaug aucuauacau agaccgcgcc augauagagc aagaauuuga ugcacuaugg      660

gcagcucaag cugcuuucaa uccggcuuua uuccaugaag uagcaagggc agaucuaaaa      720

gauacacuau uacaccaaag aaggcuaaga ccagucaaac cugguagaug uacacuguua      780

ccagaggaag aaagagcacc ucuagcacuu ccaucaacac aacgauuccg uauauaucaa      840

gaaguuaauc aucuacgccu auuaggagag gacuuacggg aaguagcauu aacacucgcg      900

caaagggaug caguugcaaa cgccuuggaa agcaaagcuc gacuaacuuu ugagcagaua      960

agaaaguuau uaaaacuauc aggaaguguu caauuuaauc uugaagaugc aaaaaggaca     1020

gaacucaaag ggaaugcaac aucagcugcc uuggcaagaa aagaacucuu cggugcagca     1080

uggggaggau uuggagaagc ucuacaagac gaaaucguau ggcagcuagu gacagaagaa     1140

ggagagggag cauuaauugc auggcuacaa gcucauacag gaguagacga agcuagagca     1200

caagcaauug cugaugcgag uuuacccgaa ggauauggua gccuaaguag aaaagcucug     1260

gcuagaauug uacccgccuu aagagcaggc guaguaacau augacaaagc cguacaagcu     1320

gcaggauucg accaccacuc acaacuagga uuugaauacg augcuggaga aguagaagau     1380

uggguucauc cagacacugg cgagauacga ccaguauuca aacaauuacc uuauuacgga     1440

aaaccacucc aacgacacgu cgcuuuugga ucagggaaac cugaagaccc agaugaaaga     1500

cgauauggaa aaaucgcaaa cccaaccguc cauauaggau uaaaucaagu ccgaacagua     1560

guaaaugcuu uaaucagacg auauggucgg ccuacggaag uaguaauaga auuagcaaga     1620

gaucucaaac aaucgagaga acaaaaagua gaagcacaaa gaaaacaagc agaaaaucaa     1680

agacgaaaug caagaauaag gaagucgauu gcagagguac ucggaauugg agaggaacaa     1740

guuagaggag cagauaucca aaaauggauc ugcugggaag aacuaucuuu cgacguugcu     1800

gaaaggagau gcccauacuc gggaguucaa auaagugcaa ggauguuauu aagugacgaa     1860

guagaaguug aacauauccu uccuuuuagu aaaacucuag acgauaguuu aaacaauaga     1920

acuguagcga ugaggcaagc gaaccggaua aaacggaaca gaacgccuug gacugcaaga     1980

gacgauuucg aagcacaagg auggagcuau gaaggaauau uacaacgagc agaaagaaug     2040

ccauuaagaa aaagauauag auuugcgcca gauggauaug aaagguggcu aggagaugac     2100

aaagauuuuc ucgccagagc acucaaugau acaagauauu uaagucgagu cgcagcggaa     2160

uauuugaggu uaguaugccc gggaucaggc acgagaguaa ucccaggaca aaugacugcc     2220

agacuaagag ggaaauuugg uuuaaacgga guucuugguc cagacggcga gaaaaaccgc     2280

aaugaccauc gccaccacgc uguagacgca ugcgugauug guguuacuga ucaaggauug     2340

augcaaagau ucgcgaacgc aagugcucaa gcaagggaag auggacuaac cagauuggua     2400

gaugauaugc cuagaccgug gccaacuuac agggcgcaug uagaaagggc uguaagacau     2460

auuugggucu cacacagacc ggaucauggc uuugaaggag caaugaugga agaaacguca     2520

uauggaauag gaaaggacgg aagcauaaaa caaagaagaa aagcugaugg cucaccaggc     2580

cgagaaauua caaaucuaau aagaauccau gaagcaacac aaccacagcg gcauggagcu     2640

ggaccagaug gacagccacu accauauaaa ggauacguag gagggaguaa uuauugcaua     2700

gaaauaacaa gaaaugauaa aggaaagugg gaaggagagg uuauuucuac uuuuagagca     2760

uauggcauug uaagaacauc aggccuaggg caacuuagac auccuagagu aggccaaaau     2820

ggaagagagc uagugaugcg uuuagugauc ggagacucag uacgauugga aauugacgga     2880

auagaaagaa caaugagaau aguaaaaaua aguggaucaa auggucaaau auuuauggca     2940

ccuauucaug aagcaaaugu agacgcucgc aauagugaua agcaagaugc auuugcguac     3000

acaucaaaau augcagguuc guuacagaaa acgaaagcca gauuugucac uauaucgcca     3060

auaggagaag uaagagauuu gggcuucaaa ggauga                               3096


<210>  4926
<211>  3099
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4926
augaaaaaaa gaauauuagg acucgacaua ggaauagcca guguagguug ggcgcuaguc       60

gaauuugacg augaauuuca agauaauaaa aaaaaaggaa caauaauaaa aagcggcgua      120

aggauauuca cacgggcaga aacaccuaaa gaaagagaau cacuagcuaa accccguagg      180

gaagcacguu cccaaagaag acgaauacga agacguguag aaagauuaaa cagcauuagg      240

gaauuauuug uaaaaaacgg aguaguggag gaauggucag uaucaaaaaa aaacccaaau      300

aauauauaca ucacccuaaa ccaaauaaag accccauggc aauuaaggau ggaaagccuu      360

gacagaaaau uaaccaauga acaguuugcu guaguccuaa cacauuuagc aaaacacaga      420

ggauauaaaa gccuacgaaa agaugauaaa gcaggaaaag uccuggaagc aauaucacaa      480

aauaaaaaaa gacuuaauga cagcgacuau cgaacauuag gugaaauguu uuguaagaac      540

gaaagcuaua aagaacacaa gcguaacaag ggcggagagu acauaaauuc aguagcacga      600

gaauuaauug aagaagaagu agaaaaaauu uucguaaaac agagagaaaa gggcaacaac      660

uucgcagaug ucaauacaga aagagaauac cuaaaaauug ccuucucaca gaaaccagcc      720

gguagcauau caaaaauggu ugguuucugc acauucgaaa acggagaaaa acgagcacca      780

agagauucau acagcgccga acuauucaaa gugcuaugua caauaaacaa cacaacguuu      840

acggauauga aaacaggaga aauaagaucc uuuagcaaag aggaaauagg aaagacucua      900

gagacaguaa aaaacgucaa agauguaaaa uaugcaaaaa uaagaaaaga ucuaggcuua      960

gaagauagcg uaaaauucag uggagucgac uaucgcgaaa augcuaaaag uaaaggcgaa     1020

ggugaaauau uuguaagcau gaaaaccuau cacgaaauua aauccaaaau agaaaaacua     1080

ucaaaagaaa aaugggauaa ccuaaagggu aauaccgaau uacuagacuc aaucguaaau     1140

auaauagcua acuugaaaga cgaugaaagu auaaaaaaag aauuccaaaa acuuaaaaua     1200

gacgaagaca ucauagaugu auuacuaaac uucgaauuca aaaaauuuau caaccuauca     1260

uucaaagcac uaagaaaaau aauaccauuc caguuccaag ggcaaaaaua cauaacagcc     1320

ucgacauccg cagguuauuc acucaagcaa gaaggaauca aaaauaaauu aguuccgcca     1380

gcaaaagaaa acauaaaugu ggcaguggua gcaagagccu uugcucaaac acgcaaagua     1440

auaaacgccg uaauaagaaa guacggacag uuugaccaga ucaauauaga acuggcaacu     1500

gaauugaaaa acuccaaauu cgaccgauca aaaauagagg agggacaaaa gaaauuccaa     1560

ucacaaaaag auaaaauauu uaaagaagua caagaacuac gcggagggau gacgccauca     1620

uccucuuuac uccuaaaaua cagauuaugg cgagaacaag acgagagaug cguauacagc     1680

ggaaggaaaa uaucuuucga agaucuauua gacaccggau uucuagacau agaucauaua     1740

auaccguaca gcaaaucaau ggacgauucc uucaacaaca aaguccuaug ucuagcagaa     1800

gaaaaucgaa acaaaaaaaa ugauauacca uuuaacuacu accaaagaau agaucgagau     1860

uggaaccuau uaauaaguau cauaacuagc augaaaaaca ugaaaauagc caaaaaaagc     1920

agauuacuaa aacaggaguu aagcgauaua gaaggcuuca uagaaaggaa uauaaaugac     1980

acaagauaug cuacaagaua uaucaaagau uuucuagaaa auaauuuaga auucaaagaa     2040

aacgaauuca uaaaaguaaa aguacaagcu cgauccggug gauuaacauc agucuuaagu     2100

uacaacuggg guaugagcaa ggacagagaa gaaaguuacu uccaccaugc uaaagaugca     2160

auaauaguag ccuguagcac acaagggaug guacaauaua uuucaaaaau aagccaacaa     2220

uacgaaaaug acaaagacaa gaugaaaaaa auaaaggaua aaacagaaaa uuguaucccc     2280

aaaccguggg acucauuccg agcugauguc gaagaaucua uagaagguau auuuguuagu     2340

uuugcgccaa gacacaaagu uacagguagc gcacauaaag aaacuauaua uagcaaaaaa     2400

caucucgaaa aagaaaggua uguuacagua aaaaagucau uagauaaaau aaaacuaucc     2460

gaccuagaaa acauaccaug uaaugaaaac ugcgguauca uacgaguucu aaaagagagg     2520

cuagaaaaau ucaaaaacga uaguuuaaaa gcauuugcag acccaguaca caugccaacc     2580

aaaugcccga acaaaaaagg accaguaauc cggagcguaa aaauaaaaga aucaaacaaa     2640

acaggcauag aagugagaaa aggauuggca gaacguggug aaaugguaag aguugaugug     2700

uuuauaaaag auaaaaaaua uuaucuaguc ccaauauacg ucucagauuu caaaaauaaa     2760

gauuuaccaa auaaagcaau cgcagcuuua aaacuugaaa augaauggau caugauagac     2820

aaaaccuaua gcuuuaaauu cucacuauuu aaagacgacc uuauaaaaau aaaaaaaaaa     2880

aaagaagaga ucuucgguua cuuuggcgga gugcaucgca gcacuggaac aauaaaaauu     2940

aagagcagac acaaaccuaa agaaauagau gaagggauag gaucacgaug ccuauuagac     3000

uucaggaagu uucaaguaga cguacucggc aacuacaccg aaguaaaaca cgagaaaaga     3060

augccugcau auauaacaag aaaagacaaa aaacacuga                            3099


<210>  4927
<211>  3105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4927
augaaaauuu uaggauucga cauagggaua gcaaguauag gaugggcauu ugucgaaaac       60

ggagaacuca gggauugugg gguucgaaua uucacaaaag cagaaaaccc aaaaacagga      120

gacucacuag caaugccgcg aagagaggca agaagcguca ggaggcgacu ugcaaggaga      180

aaagggcgau uagaaacauu aaaaagacua cuugcaaaag aaugggaucu augcuaugaa      240

gacuacauag cagcagaugg ggaacuaccc aaagcauuca uggguaaaaa ccuaacaaac      300

ccauacguuc uacgguacga agcauuacag agacuccugg gaaaggaaga gcuagcaaga      360

guaauauuac acauagcuaa acauagaggc uacgguaaua aaaacgcaaa aauaacaaaa      420

ucugaagagu caaaaagaga acaaggaaaa auauuaucag cacucgccac caacgcaagc      480

guaauugcua gauauagaac aguaggugaa uauuuuuaua aagaauucug ugagguaaua      540

aaaaaccccc aaggauuaaa caccaaugaa aauuguacgc aaccaaaagu cagaguacua      600

aaaccaaucc gcaacaaagg aggugaauac acaaacugca uacuacaaga agacuuacag      660

agagaguuac gauguauauu ugaacaucaa aaaggauuug gguuuccaau aacucaagaa      720

uuucaagaca aaauucuuaa aauagcauuu uaucaaaggu cacuuaaaga cuucucacau      780

cucguaggaa aaugcacauu uuauccugau gaaccccgug caccaaaauu cagucuauca      840

gcuauagaau uuauaacaaa agcaaagacg aucaaucucc uuguaggaau ugugaaggaa      900

aguggugaag ucugggauaa agaacaaugg agagagcguc uugauuccgu uuucagcgcc      960

gucugcgaaa gaggaaaucu aacauucgca caaaugcgaa aaauuuugaa aauagacgaa     1020

cgaauacaau uuaaagaagu agauuauacg gcuaaaaauc ccgaaaacaa aaaauuuguc     1080

gaauuuaaaa aucuaaaaaa auuuugugaa aucuuaggcg caauaguugg agacagagca     1140

cuagcugauu cuauugcgcg agacaugacc cugaucaaag augaagaaga gcuagcgcaa     1200

aaacuacgag gauauggaaa auuuucccaa gaacaaauca cacaacucgg cgcauuaaac     1260

uuuagucacc auauaaacuu aucacucaaa gcacuaagug aaauacuacc uuuuaugcga     1320

gagggaaugc guuaugauga agcaugucaa gaagccaagc uccaagcuaa gcacaacgac     1380

aaaaaaagua aguuccuacc ucccuuuugu ggaucuaucu augcggacga gcuuacaaau     1440

ccaguaguac auagagcgau agcagaauac agaaaaauau uaaacgcacu uauagccaaa     1500

uauggcaacg uucacaaaau ucacauagaa cuaacuagag auguagguaa gaauuuucag     1560

gaaagagaaa aauauaaaaa agaaauagaa agcaacuaca aagcaagagu acaagcaaug     1620

caagaaugug aaaaacuugg auuaacauua ucagagggua auauauuaaa acuacgccua     1680

uucauggagc aaaaugaaag augcguauau ucagguagaa agaucacccu agcaaaccuu     1740

aaagaacaag gggccuuaga aauagaccau auccuaccau auagcagauc cucagaugac     1800

ucauacauga acaaaguccu aguauucaca aacgaaaacc aaaacaaggg gaacaaaacu     1860

ccauaugaag cauuuggugg agacucacaa aaaugggggg aaauagaaag uuuagccuua     1920

cgaucuggau accccaaaaa aaaagcaaaa agaauacucg acaagggauu uggggaccgg     1980

gaagcuggau ucaaaucaag aaauauaguu gacucgggau auaucgcaag auuaauagca     2040

aacuauacaa aagaauaccu aaaauuucua ccacuagauu cacaugaaaa uacagcacua     2100

aucgcuggcg aaaaagauuc aaagauacau guagaagcag ucaaaggaau guuaacugca     2160

acuaugaggc auuucugggg acugggauca aaaaacagau augaacauac acaccacgca     2220

auagacgcaa uuauaauagc auauaucaac gccgcaauga uacaaagauu cagucaauuu     2280

cgacaaaacc aagagucacu aaaagcacgc uucuaugcua aagaauuagc gaaggaagaa     2340

uucaaaacac aaagagcauu cuuugagcca uuuaguggau ucagagaaca aguauuagcu     2400

aaaguagguc aaauauucgu aucccgaccu cccagaaagc gagcgcgugg agcauuacau     2460

gaagaaaccu uuuauucaau agaugacaaa aaauugucag aaacauaugg cggaaaggaa     2520

ggaguacaaa gagcgcucag ccuaggaaag auaagacaaa uaggaacaaa aauaguagca     2580

aacggaccga ugguacgagu agauauuuuu aaacauaaaa agucaggaag guuuuacgca     2640

gugcccauau acacuaugga uuucgcacug ggcauacugc caaauaaagc uguggucucu     2700

ggaaaagaca augauagaau aauuaaagac ugguuagaaa uggacaguaa cuacgaauuu     2760

uguuuuagcu uguuuaaaga ugacguaaua gaaguucaaa aaaaagauau ggagaaacca     2820

gaauuagcau auuucguuuc auuaaacacu ucaucagcaa gcauagcacu cguaaaacau     2880

gacaauaaau ucgaaacacu gacuaguaac caagcaaaau uauuuagcaa ugcgaaagaa     2940

aacaacguaa uugcagaauc auuggguaua caaaaucuca aaguauucaa aaaauauaaa     3000

guaucaccuc ucggagaaau caaagaagcc aaauacgaac caaggcaacc aauagcacua     3060

aagacaucac caaagaaaca uagaaaacaa aauaacgcac aauga                     3105


<210>  4928
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4928
augagacgau uagguuugga cuuagguaca aacagcauag gauggugccu uuuagacuua       60

ggagaugaug gagaacccgu uucaauauuc agaacaggug caagaauauu uucagauggu      120

cgcgauccua aaaguuuggg aucauuaaaa gcaacacgua gggaagcaag acuaacuaga      180

agacggagag auagauuuau acaaagacaa aaaaaucuaa uaaaugcauu agucaaauau      240

gguuuaaugc cagcagacga aauacaaaga caagcgcuag cauauaaaga cccauauccu      300

auacguaaga aagcacuaga ugaagccaua gauccauaug aaaugggaag agcaauauuu      360

cacauuaauc aaagacgggg uuuuaaauca aaccguaagu cagcagauaa ugaagcagga      420

guuguaaaac aaagcauugc agaccuggaa augaaauuag gagaagcugg agcucgaaca      480

auaggagaau uccuugccga ucgacaagcu accaacgaca cuguaagagc aagacgacua      540

aguggaacaa acgcauuaua cgaauuuuau ccagaccgcu auauguuaga acaagaauuu      600

gauacacugu gggcuaaaca agcagcauuu aaccccaguu uauauauaga agcggcacga      660

gaaagauuga aagaaauagu auuuuuucaa aggaaauuaa aaccacaaga aguuggacga      720

ugcauuuuuu uaagugauga agacagaaua aguaaagcac uaccaucauu ucagagauuu      780

agaauuuauc aagaacuauc aaaucuugca uggauagauc acgauggcgu agcacaccga      840

aucacagcua gucuagcuuu aagagaucau uuguuugacg aauuagaaca uaaaaaaaaa      900

cuaacauuua aagcaaugag agcgauucua agaaaacaag guguagucga cuauccaguu      960

ggauucaauc uagaaagcga uaaucgagau caccuaauag guaauuugac aucauguauu     1020

augcgagaug caaaaaaaau gauuggcucu gcaugggaua gauuagacga ggaagaacaa     1080

gauuccuuua uucuaauguu acaagacgau caaaaagggg acgaugaagu caggucaaua     1140

uugacucaac aauacggacu cagugacgau guugcagaag acugccuaga ugugagacug     1200

ccagauggac auggaaguuu auccaaaaaa gcaauagaua gaauauuacc aguacuuaga     1260

gaucaaggau ugauuuauua ugacgcaguu aaagaagcag ggcuagguga agccaaucuc     1320

uacgacccuu augccgcacu cucagauaaa cuagauuauu acggaaaagc ucuagcaggc     1380

caugucaugg gagcgucugg caaauuugaa gauucugaug aaaaaagaua cgggaccauc     1440

aguaauccaa caguacacau agcacucaau caaguuaggg caguagucaa ugaguuaauc     1500

agacuucacg ggaaaccaga ugaaguagua auagaaauag gcagagacuu gccaaugggc     1560

gcugacggaa aacgagaacu ugaaagauuc caaaaagaag gcagggcaaa aaacgaaaga     1620

gcaagagacg aauuaaaaaa gcuaggacau auagacucua gggaaaguag gcaaaaauuu     1680

caacuauggg aacaauuggc uaaagaaccg guagaccguu guugcccauu uacaggaaaa     1740

augaugucca uuucagauuu guucucagau aagguugaaa ucgaacauuu auuaccuuuu     1800

agucucacuu uagaugacuc uauggcaaau aagacuguau guuuucgaca agcuaacaga     1860

gacaagggaa auagagcacc uuucgaugcu uucggaaacu caccagcagg auaugauugg     1920

caagaaauuc uaggacgaag ucaaaauuua ccauacgcga aaagauggag auucuuacca     1980

gacgccauga aaagauuuga agcagaugga gguuuucugg aaagacaacu aaaugacacu     2040

agauauauuu cucguuacac aacagaauac aucucaacaa ucauaccuaa aaauaaaauc     2100

uggguaguaa cgggacgacu uacaucguua cuacgcgguu ucuggggauu aaacaguaua     2160

cuaaggggac auaauacuga cgacggaaca ccggcaaaaa aaucaagaga ugaucaucga     2220

caucaugcga uagaugcaau aguuguagga augaccagcc ggggacuguu acagaaagua     2280

ucuaaagccg ccagaagauc agaagaucua gaucuaacga gauuguucga aggacgaauu     2340

gauccuuggg auggauuucg ugacgaagua aaaaaacaca uagaugcaau aauaguaagc     2400

caucgaccca gaaaaaaaag ucaaggggcu uuacauaaug auaccgcaua cgguaucguu     2460

gaacaugcag aaaauggugc aaguaccgua guacaucgug uaccuaucac caguuuaggu     2520

aaacaaagcg auauagaaaa aguaagagau ccacucauaa aaucagcauu gcucaaugaa     2580

acggcaggau uaucaggaaa aaguuucgaa aaugcaguac aaaaauggug ugcagauaac     2640

aguauaaaau cgcuaagaau aguugaaacc guaaguauaa uuccaaucac ugacaaagaa     2700

gguguagcau auaaaggaua caaaggagau ggaaaugcau acauggauau auaccaagau     2760

ccaacaucaa guaaauggaa aggggaaaua guuucucgcu uugaugcaaa ccaaaagggu     2820

uucauaccau cauggcaguc gcaauuucca acagcucgau uaauaaugcg acuaagaaua     2880

aacgauuuac uaaaauuaca agauggcgag auagaagaga uauauagagu gcaacgacua     2940

agugguucaa aaauacuuau ggcaccacau acagaggcca auguagaugc aagagauaga     3000

gacaaaaacg acacauucaa acuaaccagu aaaaguccgg gcaaacuaca auccgcuucg     3060

gcaagaaaag ugcacauaag cccuacagga cuaauaaggg aaggauga                  3108


<210>  4929
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4929
auggaacaau uaaauuacau auuaggacua gauauaggaa ucgcaagcgu cggaugggca       60

guaguagaaa uagaucacaa ugaagauccu auccgauuaa uagauguagg aguaagaaca      120

uuuaauaaag ccgaagucgc aaaaacagga gaaaguuuaa gccuaauaag gagauuggca      180

agggggucca gaagguuggu acgucguaag aaaaugcgac uacucacagc caaaagauua      240

cuaaaaagug aaggcauacu aagcgacuua gauuucaaua aaggagcuau acuaaacuug      300

ccgaacaauu gcugggaacu gcgaguaaaa ggacucgaca agaaauuaag cgauaaagaa      360

ugggcagcag uucuauuaca uauaaucaaa cacagaggau accuaucaca aagaaaacau      420

gacagaaaaa cggaugauaa agaacuggga gcauuauuau ccggcgugag uaaaaaccac      480

ucacuacuag aagagggaga auuucgcaca gcagcagaau uagcaaucaa uguguuuugc      540

aacucaaaag gacacauaag aaaucaaugg ggagacuaua gccauacauu uuccagacua      600

gaccuccaaa aagaaauuaa acaauuauuc guaagccaac gugaauuagg gaaucccuau      660

acaucaucau cauucgaaga aaauauuaua agccuuuuaa ugaaacaaaa agaagccaua      720

ucagguaacg acauauugaa aaugcuagga aaaugcacau acgagaaaaa ugaauauaaa      780

gcagcuaaaa acacauauag cgcagaaaga uucguauggc uaacaaaauu aaauaacuua      840

agaauaguag acgauggagu cgaacgcgca uuaaacucaa gugagagaaa auugcuaaua      900

gacgaacccu auaagaaagc uaaacucacg uaugcccaag uucguaaaau acuagaauua      960

ccagacagcg cugucuucaa aggaguaaga uacuuugcug auaacgcaga gagcucaaca     1020

uuaauggaaa ugaaauuuug gcauucgaua cgaaaagcau uggaaucgaa uaaccuaaaa     1080

gacgaauggu uaguacuaaa aacaaaacca gaauuacuag acgaaauagg aacaggguuu     1140

agcauauaca aaacugauga ugauauaacc uccuaucuaa gcaacaaauu agauaaaaag     1200

guacuagaca uacucuuagu cgaaguaucc uucgauaaau ucauagaauu aaguuuaaaa     1260

ugccuuagaa aaauaauucc acuaauggaa aaaggauaca gauaugacga agccuacaaa     1320

uauaucuaug gggaaguaaa uaucgaauau aaccuuaaug acauaaagaa uccgguugug     1380

uuaagaaguu uuucccaaag uagaaaagua auuaacgcgg ucauaagauu guacggcuca     1440

ccagcuagaa uacacauaga aacggcgaga gacauuggua agaguuucaa agaaagaaaa     1500

gcaauagaaa aacaacagga gaaaaacagg uuagaaaaag uccgagcggc ggaacacuuc     1560

aaacaacuau uuccuaauua uguaggagaa cccaaagcaa aagauauucu aaaaguacga     1620

cuuuacgaac aacaacaggg aaaaugucuc uauucaggca aaauauuaga caucaaacga     1680

uuaauggaac acgguuaugu agaaauugac caugcacucc ccuuuucacg aacaugggau     1740

gauucuuuca acaacaaagu auuagucuua gcaaaugaaa accaaaauaa aggaaacaaa     1800

accccauaug aaugguucaa uggcaaagau aacucagaua gcuggaaaag auucuuagca     1860

uuaguaaaua acuccggauu cucggcagua aagaaacaac auauuuuaau gaaagaaaua     1920

gacgaggaag gauuuaaaca aagaaacuua aacgacacua gguacauaac aagauuucuu     1980

uguaacuggg uagaaaauaa caucccucuu uuaggaaaag guguaaaaag aguauuugca     2040

ccccaaggca aaauaacagc acuacuaaga ucaaauuggg gaaucagaaa aguaagagaa     2100

gacaacgacc gucaucaugc aauugacgca auaguagugg cuugcacaac uguaucaaua     2160

caacaaagaa uaacaaacua uguuaaaaac agugaaauua acagaaaaau aaaaagacaa     2220

aacuaccuua aauucccgga acccuggaaa uucuucagag aagacauaag uauccgagua     2280

uuuucuaaua acccuauuga agaacucaaa caacaacuaa gugaccgacc cgaagcuaau     2340

caccaauucg uaacaccacu auuuguauca agagcgccag ugcgaaaaau gacaggacaa     2400

gggcaugagg acaccauaag auccgcaaaa agaauauccg aagguaucag cgucuuaaga     2460

guaccacuaa cuaaauuaaa cgauaagagu cuagagcuua uggugaacag agaaagagaa     2520

aaagagcuau augaugcgcu uaaacaaaga cuccucgagu ucaaaaacga cccugaaaag     2580

gcuuuugcug aaccuuuuua uaagaaagga gggcagcaag uaaaaaccgu uagaauagaa     2640

agaguucaga aaaauggagu auugguaaac aacaacacag gcauagcaga uaauggcaac     2700

auaguaagag ucgaugucuu uuuaaagaag aagaaauacu ucuuaguacc aauauauucc     2760

uggcaaguag caaaagggau cuuaccuaau aaagcuauag uagcacauaa aaaugaaugu     2820

gacugggccg acauggacgg gucagcccaa uuucuauuua gcuuauauac aaacgaccug     2880

aucgaagucg uauuaaaaga uagcaccaua uuaggauauu ucaguaguau agaccgagcc     2940

acagcagcaa ucauaauuaa agaaccagau auggaaaaau cuaaaggaaa agaaggaaua     3000

cacagaguag guguaaaaac agccaggagc uuuauaaaau acaacguaga cguccuaggg     3060

aaaaacauuu ccagauguag aaaagccaaa agauuaccag uaagauga                  3108


<210>  4930
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4930
auguuacgga agaugcauua uagauuagca uuagaucugg guaguacaag ucuggguugg       60

gcuaugaucc gacuaaaugc aggaaacgaa cccugcgcag uaauaaaagc cggaguacga      120

aucuucagua auggaagaaa cccaaaggac ggaagcucau uagcaguuac gcgaagagag      180

gccagagcua ugagaagaag gagagaccgu uuauuaaaaa gaaaagcaag gaugauacaa      240

acuuuaauag aauauggguu cuuuccagcg aaagaagauc agaggaaagc acucgaaauu      300

augaauccau uugcauuaag agcaaaagga uuaaacgaag cauuaacacc agcagaauuu      360

gcaagagcac uauuucauau aaaucaaaga agagguuuca aaaguaaccg aaaaacagau      420

aagaaagaua acgacuccgg agcauuaaaa acugcuauua auaaacuaag ggaaacauua      480

aaaacagaga acugcaggac aguaggugaa uggcuuaaca aacgagacgu agccggacua      540

acagugagag caagauauca ccaggauaaa acaauaaaag aagaugguaa aaguaaaaua      600

aacaaguacu augauuuaua uauugacaga gcaaugauag agcaugaguu cgaugaauua      660

uggaaaaagc aagcggaauu aaauccgacu cuauucaccg aagcagcacg agcagaucua      720

aaagacguau uacuccacca gagagcucua aaaccaguua agccaggaag gugcacauuu      780

augccggacg aagaaagggc uccucuagca cuaccuucga cgcaaagauu cagaauguac      840

caagaaguaa acaauuuacg uauauuaaga gaugguuuaa aagaagaauc acuuacacug      900

aaccaaagag augaucuaau agcagcauua gaggaaaaca auaaaagaac cuuuacacaa      960

aucaagaaau uacuaggacu agguggcgua guuaaauuua acuuugaaga uccuaaaaga     1020

gaggaauuga aaggaaacac uacaagugca auauuaggaa aagacgaaca uuuuggaucc     1080

guuugguuua aauuugauga agccaaacag gacgcgauag uaaugcaauu auuuaaagaa     1140

gagaacgaag caaaacuacu aagauggcua caaaaugaaa cuggaauaga ugagaaaaga     1200

gcugaaacaa uagcuaaugu aggccugcca gaaggauacg gaucauuaug cgcuuuagca     1260

cuggcaagga uuuuacccga auuaagaaga gacguaguac cauacucaga cgcagcaaaa     1320

gcagcaggau uccaucacag cgacacaagc aauaaucaag aagucccugg auuaaccuuc     1380

ccaauagaac uuguaguaac agaaaccgga gagauaaaaa caaaacauau cuuccaugaa     1440

uuacccuauu auggagaaua cuuacaaaga cauguagggu uugggagugg caagccagaa     1500

gauagcgcug agaaacgaua ugguaaaaua gcaaauccaa caguccacau aggauuaaau     1560

caaguuagau ugguuguaaa cacacuaaua aaaagauaug gacauccaag ugaaguuaua     1620

gucgagguag cucgagacuu aaaacaaagc aaagauuuaa gagaugaaga aggaaaacgu     1680

caagcagaaa aucaaaaacg aaauaaacga auacgaacag cuauagcagg aguacuucaa     1740

acaagugaag aaagaguuag aagggacgau auagagaaaa ugauacuaug ggaagaauua     1800

ucuuuugacc cagcugauag aagaugccca uauucaggcg uucaaauauc ugcagcaaug     1860

uugcuaucug acgaaguaga aauagagcac aucuuaccgu ucucacaaac auuagacgac     1920

agccuaaaua acaaaacagu ugcacucagg caggcaaaua gaguaaaagg aaaccgaacu     1980

ccaugggaag cauucggagc ccagaguacg gccggcuucg acuaugccac aauacuggca     2040

cgagcagaac auaugccaaa aggaaagaga uaccgauucg cagaagaugg cuaccaacgu     2100

uggcuaaaag acgacgcagg auuucuaccu cgugcgcuaa acgauacuag acauaugagu     2160

aaagucgcaa gagaauacau gagccuaaua ugcccuaaua ccagaguuau accaggacga     2220

augacggcaa ugcuaagagg aaaauuugga uugaacgacg uauugggucu aaauggagag     2280

aaaaaucgaa augaucaucg acaucacgca guagacgcuu gugucauagc cguaaccgau     2340

caagguuuac uacaaagauu cgccaaagcu agugcgagcg ccagggaaca gcaacuaaac     2400

agauuagucg aaaauaugcc acuccccugg gaauccuaua gagaacaugu agaaagagca     2460

auagacgcaa uauggguaag ucauaaacca gaucauaguc augaaggcgc caugcauaac     2520

gacacagcau auggacuauu aggagaugga aaaguaucca uacauaaaau gguagaagga     2580

aaaagagaac gaaucgaaga aucauuaaaa guuaucgcuu uuacaaacgc aaaggccaau     2640

gauagacaug gacuccuaga caacggagaa ccaagaccau auaaaggaua uaagggagau     2700

aguaacuacu gcauugaaau uauaagaaau gaaaagggaa aaugggaagg ggagguaauc     2760

aguacauuug acgcauacca auuaguacga gagcauggcg uugcaagauu gagacauccg     2820

acuuugucag uaagcggaaa accuuuaguc augagacuaa ugaaagacga aacaauacgc     2880

augguaacca acgaaaaacc gauaacugca agggucugcu ggguuagaag ugauacucga     2940

auagcauucg cuucaacuuc ggaagccaau guagaugcuc gagacagaga caaaaaugau     3000

ucauucucau auauaguaaa aacagcaagu guccuacaaa aauuacaagc aagaagaaua     3060

ucaauaaguc caacaggaga gcuaagagac cccggauuca aagaauga                  3108


<210>  4931
<211>  3108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4931
auggaacaac uaaacuacau auuaggauua gacauaggaa uugcuucagu cggaugggcg       60

gucguagaaa uagaccauaa cgaagaucca auaagguuaa uugacguagg aguacgcaca      120

uucaacaaag cugaaguugc aaaaacagga gaaagccuau cacuaauaag aaggcuagcu      180

cgaggaagca ggaggcuagu aagaagaaag aaaaugcggc ugcuuaccgc uaaaagauua      240

cuaaaaagcg agggaauccu gucagacuug gacuucaaca aaggagcaau ucuuaacuua      300

ccaaauaacu gcugggaacu cagaguaaaa ggccuagaca aaaagcugag ugacaaggaa      360

ugggccgcag uguugcuaca uauaauaaaa caucgugguu aucuaaguca aagaaaacau      420

gauagaaaaa cggaugauaa ggaacuaggu gcucuacuaa gcggcguauc uaaaaaccau      480

ucacuacuag aagaggguga auuuagaaca gcagccgaac uagcuauaaa cguauuuugc      540

aauuccaaag gucacaucag aaaccaaugg ggagauuacu cacacacuuu cagcaggcua      600

gaucuacaaa aagaaauaaa acaauuguuu guaagccaac gagaacuagg gaacccauac      660

acauccucga guuuugaaga aaauauuaua ucucuauuga ugaaacagaa agaagcaauu      720

ucaggaaacg auauucuaaa aauguuagga aagugcacau augagaaaaa cgaauacaaa      780

gccgcuaaaa auaccuauuc ugcugaaaga uucgucuggc ugacaaaauu aaauaacuua      840

cgaaucgucg acgacggcgu cgaaagagcu uuaaacagua gugaacgcaa gcuauuaaua      900

gaugaaccau acaagaaagc aaaacuuaca uaugcacaag ugcgaaagau auuagaauua      960

ccagacagug caguauucaa gggaguuaga uacuuugccg acaacgcgga aagcucaaca     1020

uugauggaaa ugaaauucug gcauagcaua agaaaagcac uagaaucgaa caaucuaaaa     1080

gacgaauggc uaguauuaaa aacaaaaccu gaacugcuug augaaauagg aacaggauuu     1140

uccauauaua aaacugacga cgacauaacu agcuauuuaa guaauaaauu agacaagaaa     1200

gucuuagaua uauuacucgu ugaagucagu uuugacaaau uuauagaauu aucucuaaaa     1260

ugccuuagaa aaauuauacc auuaauggaa aaaggauaua gauacgacga agcauacaaa     1320

uacauauaug gagaaguaaa uaucgaauau aaucucaaug auaucaaaaa cccaguagua     1380

uuacgaucau uuagucaauc aagaaaagua auaaacgccg uaaucagacu auacggcuca     1440

ccagcaagaa uacacauaga aaccgcaaga gacauaggca agaguuuuaa agagagaaaa     1500

gccauagaaa agcaacaaga aaagaaccga uuagaaaaag uucgagcagc cgagcauuuc     1560

aaacaacuau ucccuaacua cguaggugaa ccaaaagcca aagacauauu aaaagucaga     1620

uuauacgaac aacaacaagg aaagugcuua uacaguggaa aaauccuaga caucaaaaga     1680

uugauggaac acggauacgu agaaauugac caugcccucc cauucucgcg aacuugggau     1740

gacucauuca acaacaaagu auuagucuua gccaaugaaa accagaacaa aggaaacaaa     1800

accccguaug aaugguuuaa cgguaaagac aacucagaua gcuggaaaag auuucucgca     1860

uuaguaaaua acucgggauu cucagcagua aagaaacaac auauacuaau gaaagaaauu     1920

gacgaagaag guuucaaaca aagaaacuua aacgauacaa gauacaucac aagauuccuc     1980

uguaacuggg uagaaaacaa uauaccccua uuagguaaag gaguaaaaag aguauuugca     2040

ccacaaggaa aaauaaccgc uuuacugcgc aguaauuggg gcauaagaaa aguaagagaa     2100

gacaaugaca gacaccaugc uaucgaugca auaguuguag caugcacuac uguaucuauu     2160

caacagagaa uaacaaacua cguuaaaaac agugaaauaa acagaaagau aaaaagacaa     2220

aacuauuuaa aauuuccaga accguggaag uucuuuaggg aagacauaag cauaagaguc     2280

uucucaaaua acccaauaga ggaacuuaaa caacaacuau cagaucggcc agaagcaaau     2340

caccaauuug uaacuccauu auuuguaagc agagcgccag uaaggaaaau gacaggccaa     2400

ggacacgaag auacaauaag aagcgcaaaa agaauaagcg aaggaauauc aguauuaaga     2460

gucccccuca caaaauuaaa cgauaaauca uuggaacuaa uggugaauag agaaagggag     2520

aaagaacuuu augacgcauu aaaacaaagg uuacuagagu ucaaaaacga ccccgaaaaa     2580

gcauuugcag aaccauucua uaagaaaggu ggccaacagg uaaaaacagu cagaauugaa     2640

cgaguccaga aaaauggagu uuugguaaac aauaacacag gcauagcuga uaacggaaac     2700

auaguaagag uagacguauu uuuaaagaaa aagaaauauu uucucguacc aauauacucg     2760

uggcaaguag ccaaagguau acuuccuaau aaagcgauag uagcacacaa aaacgaaugc     2820

gauugggcag acauggacgg aagcgcgcaa uuccuauuuu cacuguacac caacgauuua     2880

auagaaguug ugcugaaaga cucuacaauc cuaggguacu uuaguucaau ugacagagca     2940

acagcugccu ugauaauaaa agaaccagac auggagaaaa gcaaaggcaa agaaggaaua     3000

caccgaguug gaguaaaaac agcaagaucu uuuaucaaau acaauguaga uguucuaggu     3060

aaaaacauua guagaugcag aaaagcaaaa agacuucccg ucagguga                  3108


<210>  4932
<211>  3111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4932
augagaauau ucgguuucga caucggcauc acaucaauag gaugggcaua uguugaaagc       60

aaugaacuca aggauugugg cguaagaaua uuuaccaaag cagaaaaccc uaaaaacgga      120

aaaagccuag cacucccaag gcgagaagca cguggugcac gaaggagacu ugcacgcaga      180

aaggcaagac uuaacgcuau aaaacgauua cuaugcaaag aguucggcuu aaacguauca      240

gacuacuuug caaacgaugg ugaauuacca aaagcauaug aaacaacaaa agacacuaaa      300

ucaccauaug aacuaagauc ccuggcauug gaacaaaaau uagaacccaa agaacuggcu      360

agaguaauau uacacauagc aaagcaucga ggcuauggaa auaaacacgc aaaaacagga      420

ucagauaaag auaguggcaa aguauuaucc gcaaucaaag uaaaugaaac ugccaugaaa      480

gacaaauacc aaaguguagg cgaauaucua uacaaggagu ucuaccagaa acuaaggcuc      540

gaaagcgaaa agaaaggaaa caacauaaca aaagaguuca aaaacgucag aaacaaagga      600

gaaaauuacg aacauugugu cgcgcaagaa caaguacgag cugaauuaga acuaauuuuc      660

gcaaaacaaa aagauuuugg agcuacuuuc ucagaaaauu ucgaaucaca gauuauagaa      720

accgcuuucu aucaacgaga cuuaaaaagu uucgaagaua aaguagguaa augcguauuc      780

uaugaaaacg aaaauagagc accaaaagac ucacuaagug ccauggaauu uguagcuuua      840

acuagaauaa uaaauaccuu gaaaaauuug gaagagaaaa guaaaaaccu aggaaucgga      900

gaagucuaug gaaaagacaa aauacaggaa auacuuaaaa uaguccuaga uaaaggcgaa      960

augagcuaca agaaacuaag agaaauauua caccuagaug aacacauacu auucgcaaaa     1020

gacagcaaac uggacuacac ccaaggugua caagaagcag aaaaagcaaa auucaucgaa     1080

cuaaagaauc uaaaagcauu uaagaaagcc augggaggag acuuuagcaa cuucgcgaga     1140

gaagaauuag acaaaauagc aacagauaua acacucguua aaagcaaaga aaauuuagcc     1200

aagaaauuac aaaauuacag ugcacuuuca aaagaacaag uugaagcccu uucgaaccua     1260

aguuuugcga aacauauuga ccuaaguuua aaagcacuag aacaaauccu accguucaug     1320

agaggagaca aaacagguaa augucuacga uacgaugaag cagucaagaa agcagaauua     1380

caagaacaca gaaaacauaa acaaaaagga gaguuccuaa uuccgcuaaa agacuacgaa     1440

ccauacuuag caaauccagu aguggcgaga gcacuuucag aauaucgaaa aguacuaaac     1500

gcgcuauuaa gaaaauacgg aaaagugcac aagauacauc uagaguucac aagagaagca     1560

aaauuaucug caaaagaacg acaaaaauau gaaaaagaac aaaaggaaca uuuugaagca     1620

aaucaaagag caauccaaca gugugaaaua cuaggccuuc caauuaaugg aacaaauaua     1680

uuaaagauga aacuauggcu agaacaaggg gaauguugcg cauauuccgg agagaaaaua     1740

acaagagaac aucuucuaga uagcaaugcg uugcaaguag accauauuua cccauauagc     1800

agaucauuug acgauucuua uaugaauaaa guacuggucu ucgcaaaaga aaaucaagac     1860

aaaggaaaua aaacaccauu cgaagcauuu gggggcgaua aggaaaaaug ggaucguaua     1920

uuaucauuaa gcgcgaaacu acccaagcaa aaacaacgua gaaucucaaa uacaaauuuu     1980

aaagauaaag aaggaggauu cuuagcaaga aaucuaaacg auacuggaua uauagcgaaa     2040

uuagcaucac aguggaccaa agacugccua gaguucgaag accuaagaga aaaugaagua     2100

acuaucgcag gagaaaaggg aagcucaguu cauauagaag ucguaagugg ccaauuaacu     2160

agcauguuga gacacuacug gggacuagga aacaaagauc guaauaacca cuuacaccau     2220

gcugucgacg cagucauauu agcuuauaca aacaacgcaa ugauuaaagc cuuugcuaac     2280

uucagaaaga aucaagaaga aaauaaaaag aaauucuaug caaaacaaau agcagaauca     2340

gaauauaaag caaagaaagc cuucuucgaa cccugcaaaa auuuuagaca aagagucgaa     2400

gagaaaauca aagaaauuuu cguaucuaaa ccuccgagaa aaaggguacg aggagccuua     2460

caucaagaaa cauuuuaccc auuugaaaag uuaugcaaug acaaagaaua cucagaaaaa     2520

cuaaaaaugg auuacggggg agaaaaaggu augcaaaagg caauagcauu agggaagaua     2580

cgccaaauag gaacgaaaau uguaaguaau ggagccaugg uuagaguaga caucuuuaaa     2640

gauaagaaag guaaguucua ugcgguccca auauauacaa uggauuuugc acuaggcauu     2700

cuaccaaaua aagcaguugc uauuggaaaa gauaaagaug gaguaauaaa agacuggaua     2760

gaaauggaua guaauuacga cuucugcuuu agucuauuua aagaugauuu aauuuuaguc     2820

caaaagaaag aaauggaaag agcugaacua uguuacuuug uuucauucgg uaguacaaac     2880

gcacaaauaa acguagaaaa acacaauaau aaauucgaag gauuaaguga gaacgagaaa     2940

auauuauacu ccaaugcgac ccaaaaagaa guagcaggaa aaucaauagg uauacaaaau     3000

uuaaaaacau uugaaaaaua ccaagucagc cccuuagggg aaguacagaa agcagaguuc     3060

gaaccacacc aagaaaucaa acuaaaauca aguccgaaac gaaaggacug a              3111


<210>  4933
<211>  3111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4933
auguugcaca aaaugcauua uagacuugca cuugaucucg gaucaacauc acuaggaugg       60

gcaaugauaa gauuagacgc cgauaaucaa ccgugcgcag uuauaaaggc uggaguaaga      120

auauuuagua auggcagaaa uccaaaagau ggcagcucgu uagcagucac uagaagagaa      180

gcuagggcaa ugaggagacg aagagaccgu cuucucaaaa gaaaagcaag gaugaugagg      240

acacuaauug aauacggauu uuucccggca gaagcagcgc aaagaaaggc auuagaaaca      300

auuaauccau uugcauuacg ggcaaaagga cuagacgaag cucuaacacc agcagaguuc      360

ggacgagcau uauuucauau aaaccaaaga agaggguuua aaaguaauag aaaaacagac      420

aagaaagaua augauucagg ugcgcuaaaa acugcaauug gaaaagugag agcaacaaua      480

gaagcagaag gaugccgcac aguaggagag ugguuacaca aaagagacgu agcaggacuu      540

acuguaagag caagauauca ucaagauaaa guaauuaaag aggacggaaa aacuaaaauu      600

gauaaauauu augauuuaua uauagaccga gcgaugauag aacaagaauu ugaugaguua      660

uggaagaaac aagcagagcu uaaccccgca uuauucacag aggcagcgag agccgacuua      720

aaagauguac uuuuacacca aaggccguua aagccgguaa aauccggaag auguacauuc      780

augccggacg aagaaagagc gccacuggca cuaccuucaa cgcaaagauu ccgaauguau      840

caagaaguua acaauuuaag aauacucaga gacggauuaa aggaagaacc auugacuuua      900

aaacaaagag augauuuaau ugcugcuuua gagaaaaauu ccaaacacag cuuuacacaa      960

auuaaaacac uacuaggagu cgguggagca guacaauuua auuuugaaga ucuaaaacga     1020

caagaacuua aaggaaacac uaccagugca auauuaucga acgauaaaca uuucggaaaa     1080

gccugguuca cuuuugacga agcuaaacaa gaugcuaucg uacuucaauu gguuaaagaa     1140

gaaaaugaag cuaaauuagu uagauggcuu caagaugaaa cagguauaga ugaaaaacgc     1200

gcggaagcaa uugccaaugu uggguuaccg gaaggauacg gcaguuuaug cgcaaaagcc     1260

uuagcacgua ucuuacccga acuaagaagg gauguaauga cauaugauaa agcaguacaa     1320

gcagcggguu ucgaacauca cucaagacuu ggaucaaaca cugaaauccc aggauuaaca     1380

uucaaaauag aaucuauaga ucaagacagc ggagaaguaa aacaauucca uauacauaaa     1440

gugcuaccau auuauggaga auaccugcaa agacauguag guuucgguag uggcgaacca     1500

gaagacccag cagagaaaag auacggaaaa auagcaaacc caacagucca uauuggacua     1560

aaucaaauua gguuaguagu aaaugcauua cuuaaacgau auggucaucc aagcgaggug     1620

auaguagaac ucgcaagaga ucuaaaacaa aguaaagauc aaagagacga ggaaaguaag     1680

agacaagcag aaaaucaaaa acgaaauaaa agguuaagag augcaaucgc cgguacauua     1740

aaaauuucag aagaaagagu aagaagagau gauauacaga aaaugauauu gugggaagaa     1800

uuaucuuuug auccggccga cagaagaugc ccuuauucag gagcccagau aagugccaca     1860

augcuacuaa gcgacgaagu ugaaguagaa cacauacuac cuuucagcca aacacuggau     1920

gauucauuaa acaacaagac agucgcguua aggcaagcaa auagaauaaa aggcaaccga     1980

acaccauggg augcuuuugg ggcacaagca gucgcuggau uugauuacga gucuauacuu     2040

guaagagcug aaggcaugcc aaaagcaaaa agguauagau ucgcagaaaa cgguuaccaa     2100

cggugguuaa aagacgaugc aucguuuuua gcacgcgcau ugaaugauac uagacacaug     2160

uccaaaguag cauucgaaua ccuaaaacuu auaugcccca auacaagagu aauaccagga     2220

cgaaugacag caaugcugcg agcaaaauuu ggguuaaaug auguacuagg acuaaaugga     2280

gagaaaaaua gaaaugauca caggcaccau gccguagaug cauguguaau agcaguuaca     2340

gaucaaggcc uauuacaaag guucgcuaaa gcaucagcau cagcuagaga acaacaauua     2400

aauagauuag uugaaaacau gccucuaccg uggggaagcu accgagaaca cguacaaagg     2460

gcuauaaaug ccauauggau uucccauaaa ccagaucacu cucaugaagg ugcaaugcau     2520

aaugauacag cauauggauu acgaggagac ggaaagguua gcuuucauaa aaugguugaa     2580

ggaguacgua cucauagcga agauaaccua aaaguaaucg aaauaucaaa ugcaaaagca     2640

acugaccgac augggcuacu uccaaauggu gaaccacgac ccuauaaagg uuacaaaggu     2700

gacaguaacu acuguauaga gaucguuaga aaugagaaag guaaauggga aggcgagguu     2760

auaucaacau ucgaagcaua ccagcuaguc cgagaacacg guauagcucg auuacgccac     2820

ucgacauuaa gcguaagcgg uaaacuauug guaaugagac uaaugauaga cgacguagua     2880

cgauuaauaa uagaugaaca aacuagaaca augagaaucg cuacacuuag uggaaauggg     2940

caaauauuca uggcagacac aucagaggcg aauguagaug caaggaauag aagcaaagaa     3000

gaaacauuug cuuacguauc aaaaauggcc gggucauuac aaaaagccaa cgcgcgacga     3060

auaaacauaa guccaauagg agaacuccga gacccaggcu uuaaagaaug a              3111


<210>  4934
<211>  3114
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4934
auggaaagaa uuuuugguuu ugacauugga acuacaagua uuggguuuuc cgucauugau       60

uacaguucga cacagagcgc aggcaauaua caaagauuag guguccgaau uuuuccggag      120

gcgagagauc cagauggaac cccucugaac caacaacgac gccagaaaag aaugaugaga      180

agacaauuaa gaagaagaag gauacgcaga aaagccuuga acgaaaccuu acaugaagcc      240

ggauuuuuac cugcuuaugg cucagcagac uggccuguag ugauggcgga ugaaccauau      300

gaguuaagga gacggggacu agaagaagga uuaagcgccu augaguucgg uagagcaauu      360

uaucaccucg cacaacaccg acauuucaaa ggaagagaau uagaagaauc agauacucca      420

gacccugacg uagaugauga aaaagaagca gcuaaugaaa gggcugccac acuuaaagca      480

uuaaagaaug aacagacuac acuaggagcu ugguuagcgc gaagaccgcc aucagacagg      540

aaacgaggaa uacaugccca cagaaacgua guagcagaag aguucgaaag auuaugggaa      600

gugcaaagca aauuccaucc agcauuaaaa ucugaagaga ugagagcaag aauaagcgau      660

acaauauuug cacaaagacc cgucuucugg cguaaaaaua cccuaggaga auguagauuu      720

augccaggug aaccauugug cccaaaagga aguuggcuau cccaacaaag aagaaugcuc      780

gaaaaauuaa auaauuuagc aauugccggu gggaacgcaa gaccauugga cgcagaagaa      840

agagacgcga uauugucuaa auuacaacag caggcuucaa ugucauggcc aggaguaaga      900

ucagcacuca aagcacuaua uaaacaaaga ggugaaccgg gagcagagaa aaguuuaaaa      960

uucaauuuag aacucggugg agaauccaaa uugcuaggga augcuuuaga agcgaaauua     1020

gcagauaugu ucggaccaga uuggccagcu cacccaagaa aacaagaaau aagacaugca     1080

guacacgaac gcuuaugggc agcugacuac ggcgaaacac cagacaagaa aagaguaaua     1140

aucuuaucug aaaaagauag aaaagcucau agagaagcag cagcaaauuc cuucgucgcc     1200

gauuucggaa uaacaggaga acaagcagcg caauugcaag cauuaaaacu accgacagga     1260

ugggaaccgu auucaauucc cgcgcuaaau cucuuccuag cagaauuaga aaaaggcgaa     1320

agauucggag cacuugugaa uggaccagac ugggaaggau ggcgaagaac aaauuuucca     1380

cauagaaauc aaccgacagg ggaaauuuua gauaaacuuc cuucgccagc gucuaaagaa     1440

gaaagagaaa ggauaucaca auugaggaac ccuacaguug uaagaacaca aaaugaacuc     1500

agaaaaguag ucaacaaucu aauuggucuc uauggcaaac cagauagaau acgaauugaa     1560

guaggaagag acguuggaaa aucaaaaaga gaaagagaag aaauacaaag uggaauaaga     1620

agaaaugaaa aacaaagaaa gaaggcaacu gaagaccuaa ucaaaaacgg cauagcuaac     1680

ccaucgcgag acgauguuga aaaauggaua uuauggaaag aaggacaaga aagaugucca     1740

uauaccggcg accaaauagg uuuuaacgca cuauuuagag aaggaagaua ugaaguugaa     1800

cauauauggc ccagaagccg aucauuugac aacucaccac gaaacaaaac acuuuguagg     1860

aaagacguaa auauagaaaa aggaaacaga augccuuucg aagcguuugg ccaugaugaa     1920

gaccgauggu cagcaauaca gauuagacug caaggaaugg uaucagcaaa aggcgguaca     1980

ggaaugaguc cuggaaaagu aaagagauuu cuagcaaaga ccaugccaga agauuuugca     2040

gcaagacaac uaaacgauac aagauacgcu gcaaaacaaa uauuagcaca auuaaaaaga     2100

cuauggccug auaugggacc agaagcacca guaaaggugg aagcaguaac aggucaggua     2160

acagcacagu uaagaaaauu guggacauua aacaacauac uugcagauga uggagagaag     2220

acuagagcag accauagaca ucaugcaauu gaugcauuaa caguggcuug uacacaucca     2280

gguaugacua acaaacuuuc acgauauugg caacuaagag acgauccacg ugcagagaaa     2340

ccagcucuaa cuccacccug ggacacaauu agagccgaug cagaaaaagc uguuagugaa     2400

auaguaguuu cacaccgagu aagaaagaag guaucaggac cuuuacauaa agaaacaaca     2460

uauggugaca cggguacuga uaucaagaca aaaucaggua cauacagaca auuugugacu     2520

cgcaaaaaga uagaaaguuu gaguaaagga gaacuugaug aaauaagaga uccaaggauc     2580

aaagaaauag uugcagcaca cguagcggga agaggcgggg accccaagaa agcauuucca     2640

ccauaucccu guguaagucc aggagggcca gaaauuagga aaguaagauu aacaucaaaa     2700

caacaacuaa accugauggc ucagacaggg aauggauaug cagaccuagg aucaaaucau     2760

cauauugcaa uauauagguu accugacggg aaagccgauu uugaaauagu cagcuuauuu     2820

gaugccucuc gaagauuagc acaaagaaau ccuauuguac agaggacaag agcugacggc     2880

gcuucauuug ugaugucacu agcagcuggg gaagcuauca ugauuccuga aggaucaaag     2940

aaagguaucu ggauaguaca aggcguaugg gcaucaggac aaguuguauu agaacgagau     3000

acagacgcug aucauagcac gaccaccaga ccaaugccua auccuauucu caaagacgau     3060

gcuaagaaag uaagcauaga cccaaucggu agaguaagac caucaaacga uuga           3114


<210>  4935
<211>  3117
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4935
auggcaaaac aagucagaua cagauuauca cuggaccuug gaaguacaag ucuaggaugg       60

gcuauauuca ggcuagacgc ugaugcacau ccaacagcua uaauaaaagc ugguguaaga      120

auauuuucgg acggacgaaa ugcaaaagau gguucuucac uagcuguaac gaggcgagcu      180

gcaagaucaa ugagaagacg acgagacaga uuacuucgca gaaaaacccg aaugcuagau      240

gcauugcugg aauauggauu uuucccacaa gacgcagcac aaagaaaagc acuagaaagg      300

cuaaacccau augcucuaag ggccgaagga uuagcaagac cauuaacacc aggagaguuc      360

gcaagagcua uuuuccaccu aaaucaaaga agggguuuua aaucaaauag aaaaacagau      420

aaaaacgaua acgacucagg agccuuaaaa acagcuacaa gucaauuaag acaagauauc      480

caagcgucag gaugccgcac uguaggggaa uggcucugga cucaacguuu gcaaaagaaa      540

ccagaaggag ucccacauca agguguaaga gcaagauaua gagagacucc acauauaaau      600

agcgaaggua agaaaagaau agaaaagaaa uaugauuuau auaucgauag gucgaugaua      660

gaagcugaau uugacgcguu auggagugca caagcuacau uuaaucccgu uguauuucau      720

gcuacagcac acgaaaaauu aaaagauaua cuuuuauauc agcgaccgcu uagaccaguc      780

aaaccuggaa gaugcacauu auuaccagaa gaagaacgag cgccucuggc cuuaccaucc      840

acacaaagau uuagaauuua ucaagaauua aauaacuuaa gacuguuagu ugaucaauua      900

aacgaacagc cucuaacgcu ugcgcaaaga gacacaauag caacucucau ggaggggaga      960

gcaaaaauaa guuucgauca aauaagaaaa gcauuaaaac uaggaucaau gacaaaauuc     1020

aauuuagaag acgacaaaag gaacgaauua aagggaaaug caacuggauc ucaauuagca     1080

aagaaagaau uuuucggccc agcguggcac caauuugaug aggcuuuaca agacgauaua     1140

guauggcauu uacuaaguga agaaaacgaa agcgcucuca uaaguugguu acaagauaga     1200

accggaauag augcagcgac ugcacaagca guaucgaacg cauccuuacc ugaaggcuau     1260

gguaguuuau cgagaaaagc acuagaaguu auauuaccca gauuacgguc ugaaguaaua     1320

acguaugaca aagcagugca agcagcagga uuuagccauc auucacaauu gggcuuugag     1380

uacgauccug augauguaga agauuggaua gaucaugcaa cgggagaagu aaaaccaaua     1440

uuuaaaagau uaccuuauua uggaaaagca cuacaacgcc auguagcauu uggaagcggu     1500

aaacccgaag auagccagga aaaacguuau gguaaaauug cuaauccaac gguucacauu     1560

ggcuuaaauc aaguaagagu aguugcuaau gcauuaauug caagauaugg cagaccuacc     1620

gagaucauaa uugaauuggc aagagacuua aaacaaucaa gagaacaaag acagcaagac     1680

caaagaagac aagcagauaa ucagaaaaga aaugcacgua uacgaaauuc aauagcacag     1740

gcuuuaggaa ucgcugaggc ccgaguaaga cgcgaagaca uccaaaaaug gauucuaugg     1800

gaggaacuag auccaggcga cgcuaugcau agaugcuguc cauauucugg aguuccaaua     1860

ucugcaucua uguuauuauc agaacaagua gagauugagc acaucuuacc auucucuaug     1920

acucuagacg auucacuuaa uaauagaaca guaucauuaa gacaagcuaa uaggauuaaa     1980

cgcaauagaa guccauggga ugccagagaa gacuucgaag cccaagguug gcauuaugau     2040

gggaucuuac aaagagcggg acuuaugcca guacaaaaga gguacagauu cgcagcagau     2100

ggauaucaac gaugguuaag ggacgaccaa gauuuucucg cgagggcacu aaaugacaca     2160

agauaccuau caagaauagc cgcggaguac uuaagacuug uuuguccgca aggaacaagg     2220

gucauaccag ggcaauuaac cgcacuacuu cgaagaagau uugggcuaaa ugauauacua     2280

ggauuagagg gagagaaaaa cagaaaugau cacagacauc acgcaguaga cgcaugcguu     2340

auaggaauua ccgaucaugg auugaugcaa agauucgcaa gagccagugc acaagcaaga     2400

gaacaagguc ugacaaggcu agcagaagaa augccacugc ccuggccuac auauagagac     2460

cauguagaca gggcggugag cuuaauaugg guaagucaua aaccagacca uggacacgaa     2520

ggagcaauga uggaagaaac agcuuauggg cuaaugaaag auggaaccau aagacaacgc     2580

cauaaagcug auggaaccaa aggcagggaa guagcaaacg uuauaaggau ugcggaaccc     2640

gcucaaccga cuaggcacgg aguggaugac caagguagac cucuuccaua uaagggauau     2700

auaggcgguu caaauuauug cauugaaaua accagaggcg cacaagguaa augggaggga     2760

accguuaucu caacaucgga ugcuuaugga auagugcgag caagaggaau ugcaggauua     2820

cguaauacau cgcaaggaca aaauggacau ccauuaguaa ugagauuggu aauaggcgau     2880

aguuuacgau uacgaaucaa cgaugcacua caaaccaugc gaguaacaaa aauaagugga     2940

aauggucaag uauucauggc cgggguucau gaagcaaaug uagacgcaag aaauagagau     3000

aaaucucaag ccuucgcccu aguaaguaaa auggcaggcu cacugcaagc agcacaagcc     3060

cgacaagcaa caaucucucc aauaggggaa auaagagauc caggauuucc cggauga        3117


<210>  4936
<211>  3120
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4936
augaagaaaa gaauacucgg auuggacaua ggaauagcau cggugggaug ggcacuuaua       60

gaauuugacg acgaguucca agacaacaaa aagaaaggaa cgauuauaaa aaguggcguu      120

agaauauuua cgagagcaga aacgccaaaa gaaagagaac cacuugccaa accaagaaga      180

gaagcacgau cucaaagacg aagaauaagc ugcagaguag aacgacuaaa uuucguacga      240

gaauuauucg ugaaaaacgg cuuaguugca aaagaaucug ugaauaaaaa uaauccaaac      300

aacauuuaca ucacgcuaaa ucaaauuaaa acaccuuggc aacuaagaau ggaagcucuc      360

gacaggaaau uaacaaauga agaguucgcc guaguacuaa cccaucuagc gaaacauagg      420

ggauacaagu cacuaagaaa aacggacuca aaagacaaca aaacagggga aaugcuaaaa      480

gcaaucaacc aaaauaaaga aagauuagau aacagcgacu acagaacacu aggagaaaug      540

uuuuguaagc augaaagcua caaagaacau aaacggaaua aagauggaga auauauuaac      600

agcgucacua gagaauuaau ugaagaagaa gcagaaaaga uauuugccaa acaaagaaag      660

gaagggaaua acuucgcaga uguagacauc gaacgagaau aucuuaaaau agcauucuca      720

caaaaaccaa caggaaguau aaacaaaaug guaggcuuuu gcacauucga aaauggugag      780

aaaagagcgc cucgugauuc uuacuccgcg gaauuauuua aaguccuuaa uaccauaaau      840

aauaccacaa uuacugacau gaaaacaggg gaaacaagaa auuucacagg agcggaaauu      900

aaaaagauaa uagaaauaau aaaagacacc aaaggaauaa cauacgucaa aaucaagaaa      960

gaacugaaau uaggaaguuc ucaauucagu ggaguagauu auagagaaaa ugcaaaaucc     1020

aaaggagaag gugaaauauu cguuucaaug aaaacauacc augaaauaaa aagcaaaaua     1080

gaaaaacuua gcaaagaaaa auggaauaaa uuaaaagaaa auacugaauu acuagauagu     1140

auaguaaaua uaauagcgaa uuucaaagac gaugaaucaa uaaagaaaga guuccaaaaa     1200

uuaaaaauug acgaagacau aauagacgua uuauuaaauu uugaauuuaa gaaauucaua     1260

aaccuuucau ucaaagcacu uaggaaaaua auaccguucc aauuucaagg ccagaaauac     1320

auaacagcuu ccauguccgu aggcuauagc cugaaacaag aagggaucaa aaauaaauuc     1380

cuaagagugc caagucaaga agaaaacgua aacguuccag uaguagccag agcauucgca     1440

caaacacgaa aaguaauaaa ugcuguaauu agagaauaug gucaauucga ccaaauuaau     1500

auagaacuag caacagaucu caaaaauagu aaauuugaua gaagcaaaau agaaaaaggg     1560

caaaaagagu uccaaagcca aaaagauaaa guauuccaag aaguucaaga ccuaacagga     1620

gaaauuauac caucaucauc acuccuccua aaauauagac uauggcgaga acaagacgaa     1680

agauguaucu acagcggacg gaaaauauca aggaaagauu uacuagacac aggcuuuuua     1740

gauauagacc acauaauacc cuacucaaaa aguauggacg auggauucaa uaacaaagua     1800

uuaugucugg cagaagaaaa ccgaaauaag aaaaacgaca uaccauucaa cuauuaccag     1860

cgaaucggaa gagacuggaa uuuucuaauc ucaauaauag ccucaaugaa aaauaugaaa     1920

uacgugaaaa agaaaagauu acuaaaacaa gaacuaagug auauugaaau agaauccuuu     1980

auagaaagaa acauaaacga caccagauau gcaacacgau auaucaaaaa uuuccuagaa     2040

aacaaccuag aguucaaaga aaaugaguuc auaaaagcua aaguacaagc aagacaugga     2100

ggacuaacaa gcguucuaag auacaauugg ggcauaagca aaaacagaga agaaagcuac     2160

uugcaccacg caaaagacgc aauaacgguu gcuugcagua cucaaggaau gguacaauau     2220

auaucaaaca uaagccaaaa auaugaaaau ggaaaauaca aaaugaaaca aauaaaagaa     2280

cgaacagaaa auagaauccc aaaaccuugg gauucauuua aagacgacgu agaaagaucu     2340

auaaacgaca uauucguauc auucgcaccc agacauaaag uaacgggaag cgcccauaaa     2400

gaaacuauau auaguaagaa acaucuagaa aaaggauaug uaacuauaaa gaaaucgcua     2460

gauaaaauua aacuuucuga ccucaaaaac auacccugug augaaaacug cgauauaaua     2520

cgaguacuaa aagaacgacu agaaaaauuc aaugacgaca gucuaaaagc auucgcagau     2580

ccaguacaua ugccaacaaa augcucaaau aagaaaggac caauaauaag aucgguaaaa     2640

auaaaagaaa acaacaaaac aggaaucgaa guacgcaaag gguuaguaga aagaggugau     2700

augguacgag uagacgucuu cauuaaagau aagaaauauu acuuaguucc aauauaugua     2760

ucagauuuua aaaacaaaga ucuuccaaac aaagccauag uaucaucaaa auccgaaaac     2820

gaauggauaa ugauugacaa aacguacagc uucaaauuua gcuuauucaa agacgaccua     2880

auaaaaauua aaaacaagaa aggcgaaaua uucggauacu uaggaggggu ccaccgucuc     2940

accgguggga uaaauauaaa aagcugcuau aaaccaaaag aaaaagacgg uaacauagga     3000

agcaaauguc uauuagacuu uagaaaauuc caaguaggga uacuaggaga uuacaaugaa     3060

gugaaacacg agaaaaggau gccagcauau aucaauacua agaaagacaa agaacacuga     3120


<210>  4937
<211>  3123
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4937
auggaucgac gggaugcaag ggcuaugaga aggagaaggg aucgauuuca acaaagacaa       60

agggcucugu uaaaauaucu gguugcagac ggauuauuuc cugaagauga aggugagcgu      120

aaagcacuag cagcuuuaga ccccuaugca cuaagagcaa gagcauuaga ugaagcacua      180

agccuccaug aauuagggag ggcguuauuu cauuugaacc aaagaagagg guuuaaaucu      240

aaccgaaaag ccgauaggaa ggcggacgau gacgcaggaa aaauagcuau aggcguagau      300

agggcaagag aggcaauggc ggaaaacgga gccaggaccu auggcgaaug guugcauagg      360

aggagagcga gcgcuacuga ugcaaaugcu auuccuccug uaagaacaag auuaagaccu      420

gaaaguggcg aagaugcaaa aggagaugga uaugauuucu auccagguag agaucuacua      480

gaagaggaau uugcagcgau augggaagcc caagcagcgc aucacccaga cguacuuaca      540

gaugauguau augaaagauu auuugaaaua guauuuuauc agagaccuuu aaaagcacca      600

caaauuggga uguguacacu uauagcaggc gauagaagac ucccaaaggc acacccacua      660

uuucagagac ggcgucuguu agaagaacug aaugcucuaa ugauuguacg accuggagag      720

guagcacaaa gauuaacccg ugaggagaga gauacucuau ucuuaaaauu aaaagauaga      780

aacaaaguau cauuugagag uuuacgaaaa acacuaaagc uagaccccga ugcaagauuu      840

aacaaagaau cagaaaauag aacagaauug aaaggagaug aaguagcagc cgcuaugggc      900

aguaaaacaa gauuugggaa uagaugggua cauuuaucaa ucgaagaaca augggcagug      960

auagagcgau uaggagaugu agaauccgau gcagaagaug cggcguuuag ggcaugguua     1020

gcugaacgau acgaauuaac gccggagcaa acuagagcgg uaacaaaugc accucuaccu     1080

caggguuaug gaagauuugg agaaacagca acaagaaaac uaauagaugc uuugagagau     1140

ggggagaaag acggcagagu acugguauau agugaagcgg ugacagcagc aggauuagga     1200

caucauuccg acagaagaac cggugaagua uuugaagaug aagaagguag accucaacug     1260

ccauacuaug ggguggcacu agaaagacac auuaugccag gcacugguga cccaagcgau     1320

ucagacgaaa ugcgaguagg uagauuaaca aauccaacgg uacauaucgg uuugaaucaa     1380

cugagacgaa uaguaaauag auuaaucaga gcuuacggac ccccagcaga aauugcaaua     1440

gaauuagcuc gugaacuaaa guuaacagau gaggacaaga aaagaauaaa caaagaaaau     1500

aacaguaaca gacuagacgc agagagaaga agugaaaaau uauuggcaau aggcaaaccu     1560

gacacaggag cgaauagagc cuuacuaaaa uuaugggaag aacuuaaucc agcuaauguc     1620

cuagaucgaa gauguauaua uucugggguu caaauaucca ucgauaugcu auuuucagca     1680

gcaguagaag ucgaucacau acuaccauuc accgcuacau uagaugacuc uaauggcaau     1740

aaaauagugu guacuaggga ggcuaacagg gagaaacgaa aaaggagccc auaugaggcu     1800

uggggacaua gugcaaggug ggaagagaua gcggcaagag cugcacaauu gccuaggaac     1860

aagagauggc gguuugaacc agacgcgaug gaaaaauuca aagacgaugg aggauuucaa     1920

gcacgacacu uaaucgacac acaauaucuu gcaagauuaa guagagaaua uuuagccaca     1980

cuauauccug auaaaggaga aggaagcgga cacauauggg uaucuccggg ccgcuuaacu     2040

gaaaugguga gaagaaaguu aggacuuaau gaucuacucc ccgaucauaa cuuuggugga     2100

ggcgcugauc aagcuaaaaa ucgcuuagau cacagacacc acgccauaga cgcaguagua     2160

guagcaauag uagauagagg cauguuaaac aggauagcaa gagcaucggg acaggaagga     2220

gcagagggaa gagaaagaau aauaauucca gaaccuuggg aaggguuuag agaugaccua     2280

agaacagcag uuaacgcuau aacagucuca cauagaacag aucauggaac agcaaguaaa     2340

gcgggacuac ccaaaggaaa agaugcaaca gcaggacgcu uacauaauga cacugcauau     2400

ggauuuacag gagaaucaga cgcuaaagga aauucuaucg uuguuagaag aguaccauua     2460

agcagcuuua aaaagcuagc cgacauagau agaguccgag acccagagcu uaaagcagca     2520

uuauaucaac auuuagacgg ucuugaaggu aaagcauucg agaaagcaau aggugaguuu     2580

ggaucacgug aauggcaucc uuacgguggu gucagaagag ugagaguuac agaaccacua     2640

ucaguaauac cuauacgaga cagaaaugga cacauauaca aagguuauaa aggagacagu     2700

aauuacaggu augacguuug ggaauuaccu ggcggcaaau ggguaaauga aguugucagu     2760

auguuugacg cacaucaacc aggauggcaa ucagcuauca gagcggccaa ucccacagca     2820

agaaaagugc ugagucuaca uagagacgau guacuagcga ucgaaagaaa uggagcagaa     2880

cgagaauuag uaagaguagu caaauucagu gaaaaacaau uugcuguagc uccgccuaau     2940

gagggagggg cauuaaaagc uagggacgca gacaaaggug acccauuuaa guauucauau     3000

ccaagcccaa auacauugaa agcauuacgu gccagacaag ucagaaucga ugaauuggga     3060

cgaguucuag auccuggauu cccggcaaga acuaaacagc gcaaaacucg aaaaccaaac     3120

uga                                                                   3123


<210>  4938
<211>  3132
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4938
augcaaagag acaacccgcu cgucuauaga uuagccuuag aucuuggauc gacaagccua       60

ggaugggcgg uauuccgauu agaugcggaa ggaucaccuu uggcaauaau aaaagcagga      120

gcaagaauau ucagugaugg acgaaauccu aaagaugguu caaguuuagc aguuacacgc      180

agagcagcaa gagcaaugag aagaagacga gauagauuac uaaagcguaa ggcacgaaug      240

uugggagcac ucgaacguca uggauuuuuc ccacaagacg aaucagcacg aaaagucuua      300

gaaacacuua acccguuccu auuaagagcu caaggucuag aaguagcuuu aaaaccagaa      360

gaguuuggga gagcauuauu ucaucucaac caaagaagag guuuccaaag uaauaggaaa      420

acagacagga aagauucgga uucaggugca cuaaaacagg caauaggaac auuaagagcg      480

caaaugcaug aaucaggaug uagaacugua ggggaauggu ucuggaaagu uaggauggaa      540

cauaaaccag aaggagucaa aggacaagga guuagggcua gauauaggga gacaccauuc      600

acgacagaug aagggaagaa acgcauagau aaaagauaug acuuauacgu ugaccgagca      660

augguagcac aagaauuuga ugcacuaugg gcugcucaag cugcauucaa uccagcacua      720

uucaacgaag cagccagggc ugaacucagg gacauacuac uucaucagag aaaauuaaga      780

cccgugcaac cuggaaggug caccuuaguc ccagaagaac caagagcguu auuggcacuu      840

aguaguacgc aaagauuucg gaucuaucag gaggucaauc auuuaagagu ucugcaugau      900

gaacuaagag aagagacauu aacauuagcg caacgugauc aaauaguugc auuacuugaa      960

aaacaagcaa aagucacuuu uacagcaaug agaaaggcuu uaggauuuuc cgcauuauuu     1020

aaucucgaag acgcaaaacg ugauggguua aaagguaaug caacugcagc agcauuguca     1080

aagaaagacu uauucggacc uuuaugggca ggguucggga cugcucuaca agaugaaauu     1140

guaacacaac uaaugacuga agaaucggaa ccuagauuaa uugcguggcu gcaggaacau     1200

acaggcgucg augaggcuag agcucaagca auagcaaaca caggcuuacc ggaaggauac     1260

gguagcuuag gacgaacggc guuggcaaga auaguuccgg aauuacaaag ggauguaaua     1320

acauacgaua aagcaguuca agcugcagga uuugcgcauc acucagauuu gggauucucu     1380

uuugaacacg gugcugauga aguagaaagg aucggagaac guacaauagc aagcacugga     1440

gaaauaaaac ccguauuugc auucaaacaa uuaccauauu auggacgagc acuccaaaga     1500

cauguagcau uugcaaaaga uaauccaagg aaugaugagg aacgauaugg aaaaaucgcu     1560

aauccuacag uacauauagg acuaaaccag guaagagugg ucguuaaugc cuuaaugaga     1620

agauauggua gaccagcgga aguaguuaua gaauuagcca gggauuuaaa acaaaguagg     1680

gaacaaaaau uggaagcaca gcaacgacag gcagauaauc agaaaagaaa ugcaagaauu     1740

agacgaagua uagcugaagu acuagguaua ucagaagaaa gaguucgaac acuagauaua     1800

caaaaaugga ucuuauggga agaauuaagc caugacgcug cagauagaag gugcccguac     1860

ucugguguac aaauaucagc uucaauguua cuaggagaaa gaguagaaau agaacauauc     1920

cuuccauuuu cuaugacacu agaugauuca uuaaauaaua ggacaguggc auuaagagaa     1980

gcaaaccgua ucaagagaaa cagggccccc ugggaagcca gagcagauuu ugaagcccaa     2040

gguuggagcu augaugggau ucuacaacga gcagaacgua ugccacuacg aaaaagauau     2100

agguuugcac cagaugguua ugaacgcugg cuaggagaag auaaagauuu ccucgcucgu     2160

gcacuaaaug auacuaggua uuugucaagg guugcagcug aauauuuaag gcuagugugc     2220

ccaggaucag cgacaagggu aauaccagga caaaugacag cacuauugag aggaaaauuu     2280

gguuuaaacg auguacuagg uuuagauggu gaaaagaaua ggaaugauca uaggcaccac     2340

gcuguagaug cauguguaau uggaguuaca gaucaacgga ugaugcaaag guucgcucaa     2400

gcaaaugcgc aggcaagaga agacggauua acuagauugg uugaagacau gccccuaccu     2460

uggcccaccu acagagauca ugucgaacga gcaguucauc auauuugggu uagccauaaa     2520

ccagaccaug gcuuugaggg agcaaugaug gaggaaacau cguauggaau uuuaagagau     2580

ggcuccauaa aacaaagaag aaaagcagau ggaagcgcag gaagagaaau aaguaaccuc     2640

aucaggauug ccgaaccagg ucaaccccaa aggcauggag uagaugcuga aggacgcccc     2700

uuaccuuaca aaggauacgu uggagggagc aacuauugca uugaaauaac aguaaaugau     2760

aaagguaaau gggagggaca agucauaagu acguucgaug cguauagaau agucagagaa     2820

ggcgguuuaa gacaacuaag acauccuaca caaggucaaa acgguagggc auuagcuaug     2880

agauuaguua uaggggauuc aguaagauua gaagcugggg aaggaaguuu acaaacauua     2940

agaguugcaa aaaugucagc agcgggacaa guauuuauga gcgagguuaa ccaagcaaau     3000

guugaugcaa gaaauagaaa uggggauuua ccuuauguau ccaagauggc aggaaguuuu     3060

cagaaagcua gagcucgcca aguaacaauc ucuccuauag gagaacuaag agauccugga     3120

uuuaaaggau ga                                                         3132


<210>  4939
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4939
augaaauaca cauuaggucu agacuugggc uacaacagca uagguugggc aauacuugaa       60

aacgacgaaa acggaagccc gauaaaauua uucaaugcag gaguacauau auucgaaccu      120

ucaaucgaag acauagagau agacgggaaa ggcaaaacca aaaaugucaa aagaagagag      180

gccagacuca gaaggcgaca acuauucaga aaaacuaaaa gacucgaaaa acuccuaaau      240

uuuuucaucc aggaaggauu uuuccaaaau cagaaaguau uaauaaacga ucccuucgaa      300

cgagcagaau ucuuauauca acuagauaaa aaaagcagaa acccauacau acuacgcaga      360

gaagcacuag acaacagacu aagccuagaa gaaauagcaa gaaucuucua ucauauagcc      420

caacacagag gauucaaaag cacaaaaaga gaacuugccg aaaugaauaa agacgaaaaa      480

aaaaaaaaag aagacggaau aauucaaagu gaaaucaaca cacuaaagga agaaauaaaa      540

aaaaauaguu guagaacucu aggagaauac uucgccaaac uauuugaaaa cggagaaaaa      600

auacgggguc gucauacuga cagaaaaaug uaugaagaag aauuccaaaa aauuuuuaac      660

aaacaaaagg aauucuaccc caauauauuc acugacgaau ucaaaaaaaa acuauauaaa      720

uuaaucuucc aucaacgaga acuuaaaagu caaaaagaua aaguuggcuu cugcuccuua      780

gaacgaggaa aaaaaagagc accacuggca cuacuuguaa gucaacgaua cagauacaug      840

ugcaaaguua acaaccuaaa cgucauagac accgagacug gagagaaccg agaauuaaca      900

guagaugaaa aaaaaaaacu cuacgaaaaa cuagaaaaca gcgaaaaacu aagcuuuaaa      960

gagauuaaaa aaauccugaa ccucucugag aaaagauaca uauucaaccu cgaaaaaagc     1020

gaaaaguaua uuauaggcaa caaaacaaau guaaaaauga aaaacaucau caaagacugg     1080

gacaacuucg acgaggauaa aaaaaacuuu uuuauaaaug aggcacgcac ugugguaaaa     1140

auagaaaccu uagaaagacg gggaaagaaa cuaggacuca gugaugaaga aagcaaagaa     1200

uucagcaaag uaguauucga agauggauau uuaaacuuca guaaaaaagc aguagaaaaa     1260

uuauuaccau accuaaaaga aggaaaaaag gaauacgaag cgauaaaauu agcauaucca     1320

gaaaauuccg uucaaaauga uaaccuaaaa gagcuggauu uccuaccacc aauaaaaucc     1380

aaagaacuga aaaaauacuu cgagguacga aacccaauag uagaaaaagc guuaacagaa     1440

acacgauacc uaguuaacuu ccuaaucaaa aaauacggca aaccggaaac cauaaaaauc     1500

gaacuugcuc gcgaauuaaa acuuucgaac aaacaaaaag agcgaaaaca caaagaaaac     1560

caagaaaacc guaagaaaag agaagaaguc auagaaaaac uaaaaaacga cuuaaaaaua     1620

ucagaaccga cagaaacaca aauccaaaaa uaccuuuugg cagaagaaug cgacuguaaa     1680

ugccccuaua cugguagauc uaucucaaug gacaguuuau ucggagaaca cccuuuguuc     1740

gacauagaac acauaauccc guuugaucga agccuagaca auucguuccu aaauaaaacu     1800

cuauguuaca gcgaagaaaa uagaaaagua aagcacaaca aaacacccua ugaagccuau     1860

uauggaacag agaaauggga ggccauaauu gaacgaguga agaaguucaa aagcaacgua     1920

agaaacgaaa aauuaagaag guuccaaaug accaaagaag acguagaaaa cuauauacaa     1980

aauuuuucaa cacaaaaucu aaacgauaca aaauaugcaa gcaaauuagc caaaaaauau     2040

cucggacuac uauauggaag cauaaacgaa gacggcauag auaagaaucg uaagagaaga     2100

auuuuugcau caucgggaaa gguaacaucc acgcuacgau acgcacuagg gcuaaauacc     2160

auauuaaacg acgaaaacau caaaacacgc gaagaccaua gacaucaugc aauugaugcc     2220

auaacaauug cauuaacaga caguucuaug auaaaaaaaa uaucagaugc uagcaaaaga     2280

gaaaaaaaca uaaauagcuu auucaaagau uuccaagcuc ccugggaaaa uuucagaaaa     2340

gaagucgaag agauaaucgc aaaaaugaau auaucgauaa gaauaaauaa aaaaguaucc     2400

ggacaaauac acgaugaaac auucuauaag aaaccguuua aagaagaaga gaaaagcuau     2460

acacaccaac gaaucaaccu cgaggaacua acaauaaaag augaagacaa gaagaagcaa     2520

ucuuccaaau ugaucgacaa cauuguagac ucuaaaauaa gaaauauaau aauaaauagu     2580

cuuaacggag aaaagccaaa gauuagguuu aaggacccaa aagaccaccc auacaucauc     2640

cagaaaaacg gaaaaaaaau acaaauacau aaaguaacca uuaaaacaaa gcucgaaaca     2700

uacaaugaaa uaggaccaga aaaagcaaaa agauacguaa aaucgaguga cagucaccac     2760

aucgaaauau ucgaagacuu aaaaaccaag aaaugggacg gcuacgucgu aagccuacua     2820

gaagcauacg aacgaaaaaa aaaaaaauua ccaaucguaa acaaaaaccu aggcgaaggu     2880

aaaaaauuuu uauucagccu agcgaaagga gaaaucauag aacuugacac agaaaaugga     2940

aaacgggaac uauucguugu aagaauaauc ucggccucaa auaaucaaau uaaguucaca     3000

agaaucaacg acucacguaa aaucaccgac auaccagcaa cgggccugac agcuugccca     3060

uccagcuuaa agaaaagaaa cugcaaaaag guagucauaa acauacuagg cgaaaagcac     3120

uacaguaacg auuga                                                      3135


<210>  4940
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4940
auggcagaaa agaaauuuaa auaccgguug ggauuagacc uaggcacaaa caguaucgga       60

ugguguaugc uaagacuuaa caaagacaau gaaccagcag caguuauaaa aucuggaguc      120

aggauuuucc acgacggaag aaacgagaaa accaaagaac ccuuagcagu aguuagaaga      180

aacgcucgaa gcaucagaag aaaucuagau cgaaaaaucu cuagacguaa ucaucuuuua      240

aacacauugg ucaaauauuu ucuacuucca agcaaugaaa ucgaaagaaa agcccuagcu      300

guacuaaacc ccuacgaauu acgaagucga gcaguaaacg aaagauuaga acuauucgaa      360

cuaggaagac uacucaugca ccuuucaaaa agaaggggau ucaaauccaa uagaaaagua      420

gauaaagagg acagcagcgg caaaauuaag ccagcaauag agagacuuaa aaacaaaaug      480

caagaaacga auaagaaaac cgcaggggaa uaccuguaua gccucaugca aaacggaaaa      540

ccaguuagag caagauuagg cagaauugau ggaucaaaug gcuacgagau auauuuagau      600

agagcacuaa uagaagagga auauaacuua auuaugaaug aacaaaagaa acaucacaaa      660

gaauuaacag acgauaucau aaacgaaauc uuuaacauca uauucuacca aagaccacua      720

aagaaacaaa uaaucggcaa augcagucuc accggagaag acacaaaacu aaagaaagca      780

cacguucuag cacaagauuu uaucuuguua caaaaaguac aagaccuaag aguauucgac      840

gacaaccaac uugaguuccg accauuaagu aaugaugaaa aagacauaau caaaagagaa      900

uuacgcaaaa agaaagaagu aaguuacgaa aacaucagaa aauuacucaa gaaauacuau      960

gaaaacguua aauaugguau guucucgcau gaaacgcuau auaagaaacu ucaagggaac     1020

aaaacaaaug cacuuauguc agauaaagaa aucaucggag acagcuggua uucacuaucc     1080

cccgaaguac aguacgauau cauagaggaa cuauauuccg acaaagacaa ugacgaguua     1140

agcaaacucu auucagaaaa auucaacuua aaugaaagcc aaauaaacgg auuguuaaac     1200

aaagccaucu auagacuuga cagcgguuau auaagauaug gcaaaacagc agucacaaau     1260

uuaaauucaa ucauggacaa agacaauuua aaccuucaug augcaagaga agaagcagga     1320

uacgaaaaag aaaauaaaca agaugcaucc gaauaccuag aguacuacgg caaaguauua     1380

ccggacuccg ucguugaucc ucaaauagaa aauccuaaaa acgacgaaga aagauaugga     1440

aaaauugcaa acccaacagu acaugccgcu cucaaucaac uaagaaaauu aguaaacgaa     1500

cuaauaaaac uauauggcaa accagagcaa aucguaauug aacuagcuag agaccucaaa     1560

aacucaagaa aagcaaaaga uguuaucaau aagaaaauug ccgaaaaccg aaaauuaaau     1620

gaagaagucg auaaaacauu acaagaauau ucagaaaaau acaauacaca cauagugaaa     1680

aacaaauuaa auagagauaa aguaaaacua uggagggaau uaggaaaaga cccauuagaa     1740

agaaaaugca uauacaccgg agagcaaaua aauauagcaa aacuauuuuc ugacgaaguc     1800

caaauagaac acauaguccc auacucuaaa acauuagacg auucauuuaa caacaaaaca     1860

uuauccauga aacaagcaaa cuacuacaaa ggaaauaaaa caccauacga agcauuuaaa     1920

gacaguaaag auggauauaa cuaugaugac aucauagcgc gggcaaaacu ccuaaacaac     1980

gacaacaaau uuaaacgguu cacaaaagau gcaauggaca uauauaauag agauggcaaa     2040

gauuuuauag cccgacaacu uucggacaca caauaucuau ccacacgagc auuaauuuau     2100

uuacagagcu uauacgaaaa agcacaccac aguagcauau ggacaauacc gggacaacuu     2160

acagcauuaa uaagaaauua ccuuggucua aacacccuaa uaucaaacga uaaaaauaaa     2220

aacagaaaug aucaccguca ccacgcaguc gacgcauucg uaguagcagu cacaaguaga     2280

ucccuacuac agaaaauauc auaugccgcu ucacuccaag aagaccuaga caauagaaau     2340

guaaguaguu acagaaacaa acuauuagag aaaaugccug aaccguuccc aaacuaccga     2400

aaaucucuag aacuagcuau cgacaacauc aaaacaucac acaaagcuga cagaucaaua     2460

aguggaaaac uccacgaaga uacagcauau ggaauaguag aaaacgacga caaauacaau     2520

guuguaacaa gauggacugu ugacaaauuc aaagaacaaa aacacuucga aacaauaaga     2580

gacaaugagc ucaaagagau cgcauguagu gacaaagaac uauucgcaaa aaucguggaa     2640

accagaaaaa ucaaaucuau aagaaaacuc gccaaagaaa accccguaau aaaaaucaaa     2700

gauaaauccg gcagagauua uaaagcauuu gcaggaggaa acaacauuug cguagaaaua     2760

uacgaagucg auggagucag aaaaagcgaa guuauccaau uauuugaugc ugcacaaaaa     2820

ggauuuaugc cggaauggau gaagaaauac ccaaacggua aacuauugau gagacuauuc     2880

aaaggcgacg uaaucggauu uauagaaaac agaaaauaua auuauuacac aauaaaaggg     2940

aucucaauag cagaucaaaa ccucaaauug cuagccauca auaaagcaga ggggauauuc     3000

aagaaagguc uuaacauacu auucgauucc aauaauaaaa auagcuuaaa cgcaaaacaa     3060

uucaacauau caguaacagg aaucguaacu aaaaagaaaa cagccgaugu aaauuucugg     3120

aaaaacagaa aauga                                                      3135


<210>  4941
<211>  3135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4941
augcuacaca aaaugcgaua cagauuagcu uuagaccuag ggucuacuag ccuaggaugg       60

gcaaugguca ggcuuaaugc aaaccaacaa ccaugugcag uuauaaaagc uggcguacgg      120

auauucucua auggaaggaa cccaaaagac ggaaguaguc uagcaguaac uagacgagaa      180

gcaaggucaa ugcguagaag aagagauagg cuauuaaaaa gaaaggcccg caugaugaga      240

acgcuuauug aauacggauu cuucccagcu aacgaaacgc aaagaaaggc acucgaaaau      300

uuaaauccuu augcucuuag agccaaggga cuggaugaag cguuaacucc aagugaauuu      360

gcuagagcac uauuccacau aaaccaaaga agaggguuua aaucaaauag aaagacugac      420

aagaaagacu cggauagugg cgcgcugaaa acagcaauaa aacaauuaca uucaguuuua      480

gacccacaag gaaaugacgg caagccaaga acaguaggag aacuacuaua uaagcgauuc      540

acagauuuaa guaaacuacc aaaagaucga acaguacgag cuagguauag acaagacaaa      600

auaguuaaag acgacggaaa aaccaaaaua gacaaauauu augauuuaua uauagauaga      660

gcaaugauag aacaagaauu ugaugccuua uggaagaaac aaucagaauu aaaccccaua      720

uuguucaccg aaaaugcuag agcagaccua aaagacguac ugcuauauca aaggagucua      780

aaaccaguaa aaccgggcag augcacauuc augagugaag aagaaagagc accccucgca      840

uuaccaagua cucaacgguu uagaauguau caagaaguua auaaucuccg aauacuuaga      900

gagggauuaa aagaagaauc auugacauua cagcagcgag acgauuuaau uaacgcacua      960

gagaaaaaca acaggaggac cuuuacacaa auaaagaaac uauuaggagu agguggauca     1020

guacaauuua auuuugaaga cccuaaaaga gaagaacuaa aaggaaauac gaccagcgca     1080

auauugggua aaagcgaaca uuucggggaa gcaugguuug cauuuaacga agcaaaacaa     1140

gaugcaauag uacuccaauu aauaaaagaa gagaaugaag caaaauuagu acguugguua     1200

caagacgaaa cugguauaga cgagaaaaga gccgaaguca uagcaaaugc cggauuacca     1260

gaagguuacg gaucauuaug cauugaagcc cuagcacgaa uuuuaccuga acuaagaaga     1320

gauguaguua cauaugacaa agcagugcaa gcagcaggau uugaacacca cucaaaauug     1380

aauagaaaug aagaaauacc agauauuaca uuuaagauug aaucaauaga ucgaaauuca     1440

ggagaaauca aacaauuuca ucuacacaaa gaacuacccu auuacgguga auacuuacaa     1500

cgucauguug gauuugguuc aggcaagcca gaagacucaa uagagaaaag auauggaaaa     1560

auagccaacc caacagucca cauaggacua aaucaaguaa ggguaguagu aaaugcacug     1620

auuaaaagau acggacaucc aucagaaaua auuguggaag uggcaagaga uuuaaaacag     1680

aguaaagaua gaagggacga agaaaauaaa agacaagcag aaaaucaaaa gagaaaugaa     1740

agacuaagaa aagacaucgc ugacauauua ggaauuucgg aggaacgagu ccgucgggau     1800

gauauagaaa agaugauauu augggaagaa uuaucuuuug auccagcaga ccgcagaugu     1860

ccauauucag gcguccaagu cucaacagua augcuacuaa gugacgaagu agaaguagaa     1920

cauauauuac cauuuucaca aacucuagau gauaguuuga auaacaaaac cguagcgcua     1980

agacaagcua accgaauaaa aggcaacaga acaccauggg aagcguucgg aacauccaac     2040

auaucgggau ucgacuacuc aggaauauua acacgagccg aacuaaugcc gaaagcgaaa     2100

agauauagau uugccgaaga uggauaccag agauggcuaa aagaugacgc gggauuccug     2160

gcaagagcuc uaaacgacac cagacauuug ucuaaaauug caagagaaua uaugucauua     2220

auuugcccaa auacacgcgu aauucccgga caaaugacag cuaugcucag aagaaauuuc     2280

ggacuuaaug acgugcuugg gcugaauggc gagaaaaaua gaaaugauca caggcaucau     2340

gcaguagacg ccuguguaau agccgugaca gaucaaggau uacuacaaag guuugcagca     2400

gcaagcgcua gcgcuaggga aagacaacuu aaucgccuag uugagaacau gccucuaccg     2460

ugggagucau acagagaaca uguaagacga gcgauugaug gaaucugggu cagccacaga     2520

ccugaucaua gccaugaagg ugcuaugcau aacgauacug cauacggcuu gaggggaaau     2580

gggaggguau cguuucacaa aaugguagag ggaaaaagag aguauauuga agacaacuua     2640

aaaguaauag aaauaacaga cacuaaagca auagacagac auggauuacu cccaaccgga     2700

aaaccaagac cauauaaggg auauaaaggu gacuccaacu acugcauuga aauaguaaga     2760

aaugaaaaag gcagauggga aggugaaaua auaucaacau uugaugcgua ccagcuaguu     2820

cggaaguacg guauagucca acuaagauau ccgguauuau caguaagugg uaagccccua     2880

guaaugaggc uuaucaggaa ugacuuugug agauuagaac augaaggcau aacaagaacc     2940

uuaagaguau guaaaaugag agaagacgga caggucgcau uagcagaugu gacagaggca     3000

aacguagaug caaggacuag aaccaaagaa auauguuacg ucuuuaaaac aggagggucu     3060

cuacaaaagg caaaagcaag gagaauuaca auaaguccaa uuggagaauu gagggaucca     3120

ggcuuuaaag aauga                                                      3135


<210>  4942
<211>  3138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4942
augcaaaaag aaaaaccauu agcauacaga cuagccuugg auuuagguag uacaucucua       60

ggaugggcag uauuaagacu guccgcgcca ggagagccaa gcgcagucau uagagcaggu      120

ucaagaauau uuagugacgg gagaaaucca aaagauggaa gcucgcuagc agugacgaga      180

cgagcugcaa gggcaaugag aagaagaaga gaucggcucu uaaaaagaaa agccagaaug      240

uuagcugcgu uagaaagaca uggauucuuc ccacaagaug aagcugcaag aaaagcucua      300

gaaaaacuaa acccauaugg acuuagggcc agaggauuag aaucagcacu aaagccagaa      360

gaguucggua gagcucucuu ucaucucaau caaagaagag gauuucaaag uaauagaaaa      420

acggaccgaa aagauucaga uucaggggca cuuaaacagg caauagggac acuaagagcc      480

caaaugcacg aaagugguug ucguacaguu ggagaauggu ucuggaaagu gagaauggaa      540

agaaaaccgg aagggguaaa aggacaaggc guaagagcaa gauauaggga aaccccuuuu      600

acaacagaug aagguaagaa acguauagau aaaagauaug accuauaugu cgauagagca      660

augguagcac aagaauuuga ugcauuaugg gcugcacaag cuacuuucgc accagcccua      720

uucaaugaag cggcacgugc agaacuaaga gacauauuau ugcaccaaag aaaauuaaga      780

ccuguacagc cagggcgaug uacuuuagua ccagaagaac cucgagcucu uuuggcauua      840

ccgucaacac aaagauuuag aaucuaucaa gaaguaaacc aucuaagggu acuucacgau      900

gaacuaaggg aagaagcauu aacuuuagcu cagagggacc aaauaguugc auuauuggaa      960

aggcaagcaa agguaacguu uacagcuaug agaaaagcac uaggcuucuc cgcauuauuu     1020

aaucuugaag acgcaaaaag agauggacua aaaggcaaug cgacaacagc ggcacuagga     1080

aagaaagauu uauuuggucc uuuaugggcu gguuuugaua cagcuuuaca ggaugaaaua     1140

guaacccaau uaaugacaga agagucagaa ccuagacuga uagcauggcu ccaagaacau     1200

acaggaguag augaggcgag agcucaagca auagcaaacg caggauuacc ggaaggcuac     1260

ggaaguuuag ggagaacggc uuuagcaaga aucguacccg aacuacaaag agauguuaua     1320

accuaugaua aagcaguuca agcugcugga uuugcacauc auucagaccu uggcuuuucu     1380

uuugagcaug acgcagauga aguagaaaga auaggugaac gcacuauagc gucaacaggu     1440

gaaauuaaac cuguauuugc auucaaacaa uuaccguauu acggccgagc acuacaaagg     1500

cacguugcgu ucgccaaaga aaauccaagg aaugaugaag aaagauaugg uaaaauugca     1560

aauccuacug uucacauugg acuaaaucag guaagaguag uaguaaacgc acuaaugugu     1620

agauauggaa gaccgacaga aguagugaua gaauuggcac gagaucugaa acaaucuaga     1680

gaacagaaau uggaggcuca gcaaaggcaa gcagacaauc aaaaacggaa cgcuaggaua     1740

agacgaagca uugccgaagu uuuggggauu agcgaagaaa gagucagaac acuagacaua     1800

caaaaaugga uacuguggga agaauugagu caugacgcag cagaucggag auguccauau     1860

ucaggaguac aaauuucagc aucaauguua uuaggagaaa gagucgaaau ugaacauaua     1920

cuuccauuuu cuaugacauu agacgauagu uuaaauaauc gaacaguggc auuacgagaa     1980

gcaaacagga uaaaacgaaa uagaacacca ugggaugcaa ggacagauuu ugaggcccau     2040

gguuggucau acgacggaau uuuacaacgc gccgagagaa ugccacaaag aaaaagacac     2100

cgguuugcac cugauggaua ugaaagaugg cucggugaag auaaagacuu ucuagcucgu     2160

gcauuaaaug auacaaggua uuuaucuaga guagcagcug aguacuuaag auuaguuugc     2220

ccaggaucug caacacgagu uauaccagga cagaugacag cucuacuuag aggaaaauuc     2280

ggauuaaaug auguuuuggg cuuagauggg gagaaaaacc gcaaugacca uagacaucac     2340

gcaguagacg ccuguguaau aggagucacg gaccaaagaa ugaugcaaag auuugcgcag     2400

gccaaugccc aagcccgaga agauggucuc acacgauuag uugaagacau gccucuucca     2460

uggccaacau acagggcaca uguugagcga gcaguaaggc acauuugggu aagccauaaa     2520

ccagaucacg gcuucgaagg agcuaugaug gaagaaacua gcuauggaau acuucgagac     2580

ggaacaauaa aacaaagacg caaagcugac ggaucagcag gccgagaaau caguagauua     2640

aucagaauaa cagagccaag ccaaccgcaa cgacaugggg uugaugcaga aggaagacca     2700

cuuccauaua aaggauaugu gggcggaagc aacuauuguc uagaaauuac cguuaaugau     2760

aaaggaaaau gggaagguca aguaauauca acauucgaug cauacaggau ugucagagaa     2820

ggaggauuga gacaacuaag acauagcacu caaggacaaa auggacaagc acuugccaug     2880

agacuuguua uaggagacuc aguaagguua gaagucgacg aacaagaacg cguaaugaga     2940

aucguuaaga uaaaugguaa uaaugggcag gucuucaugg ccccaguaca ugaagcgaau     3000

guugaugcaa gaaauuccga uaaaaaugaa gcauucgcau acacaucaaa gauugcaggc     3060

agcuuucaga aaacaaaggc aagacaaguu acaaucaguc ccauuggaga auuaagagau     3120

ccuggguuca agggauga                                                   3138


<210>  4943
<211>  3138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4943
augaaaccaa gaaaccugaa cuauaucuua ggacuagauc uaggcauagc aucaguuggg       60

uggagcguug ucgaaauuga cgagaaugaa uaccccauaa gacucauaga uguaggugua      120

agaacauuug aacgagcuga agucccuaaa acuggcgaaa gccuagcacu agcuaggaga      180

cuagcuaggu ccaccagaag auuaauacga agaagagcgu ucagauuauu aaaagcaaaa      240

agacuacuaa aacaccacca aauagucaau gcagaagaau uaacacagcu accaaaucaa      300

ugcugggagu ugagagugaa aggacucgau ucacuacucu cuaacaccga augggcagcc      360

guguuacucc aucuacuaaa gcauagagga uauuuaaguc aacgcaaaaa ugaagcacaa      420

aacgcagaua aagaacucgg aaaacuccgu gaaggaaugg acaacaacuc aaaacuguua      480

cuagaaaaca auuacagaac uccugcugau auugcaguga aaaaauucgc cguagaagaa      540

ggacauauga gaaaucaaag gggggcauau acccacaccu ucaacagauu agacauccuc      600

gcagaaaugc aacuauuauu caaaacacaa agagagcuug guucuacaua ugcaaauggu      660

gaacuagaac aagcuuuuug ugaauuauua uuauggcaaa aacaagcacu aaaccgaagc      720

caaauguuaa gucuaguugg aaaauguacc uucgaaaaag aagagaaaag agcagcaaaa      780

gcaucauacu cugcagaaag auuuguagca auucaaaaac uacaaaauuu acggauccua      840

gaaaauggag aagaaagggg auuauucgau agcgaguucu ccuuacuacu agaaaacgcu      900

uacaacuuaa aaucaggacu uacauauaag caaguucgua aaauccuaag uuuaagugaa      960

aacgcuauau ucaaaggacu accguauuua ucugaugacc uggaaaaacc agagaaaaca     1020

caauucuuag cguucaaauu uuaucaccaa cuggcagaua ucuuaaaaaa caauggauuu     1080

ucagaugaau ggcaaaagcu aucacaagag ccaaccuuac uagacaaacu aggaacagaa     1140

uuaucacuau guaaagaaga aaaugaauuc auagcacaau ucaauggaga auugccagaa     1200

gcaauguuau cuacgcucuu caaccauacc aauuucgaca aauuuauaca uauaucauua     1260

aaagcacuua auaacauacu accacuaaug gaacaaggua augauuacac aaaagcuugg     1320

cguaaaguau acccggaacc aacgaaaaaa gaugaaaaaa cacucccacc aauuccagcg     1380

gaugagauua gaaacccggu agucuugaga agccuaagcc aggcgagaaa ggucauaaau     1440

gcgguaauca gacuauacgg cucaccugcc cgaauacaca uagaaacagc ccgugaacua     1500

ggaaaaucau augacgaucg ccaaaaaaua aaaaagcaac aagacaaaaa uucagaugaa     1560

agagaccaag caguaaaaaa auuccucgaa gaaugcccua acuuugcaaa caaaguaaaa     1620

ggcaaagaca uauuaaaaau aagacuauac auaaaccaag auggaaaaug ucuauacucg     1680

gggaaaccac uagauccaca uagacuauug gaaauaggau auguugaaau agaccaugca     1740

cuaccauuuu ccagaacgug ggacgauuca caaaacaaua agguccuagu acuagcaaac     1800

gaaaaucaaa auaaaggcaa ccaaacacca uuugaauggu uagguaaaga cgaacaucaa     1860

ugggcacugu uuguagcacg aguaaacgga ugccgauucc cauacgcaaa aaaacagagg     1920

auacuuacaa aaaaauuaga cgaacaaggu uucuugaaaa gaaacuugaa cgauacacgg     1980

uacguauccg ccuacuuaau gaaacacaua aaggaaaacc uacaccuagu uggcaaaggg     2040

agugauaaag uuuucgcauc caacggccaa guaaccaauu uucuaagacg auguugggga     2100

uuagaaaaga aaagagaaga aggugacaga caucacgcac uagacgcaau cguugucgcg     2160

uguucaacgg cuucaaugag gcaaaaaauu acauuauuua agaaauacca aagauggaau     2220

uuaaaaacug gaaaacauau ugaucaagag acaggcgaaa uaauaccacu acacuuccca     2280

gcuccauggg auuucuucag acaagagguu augaucagaa uuuucaguga aaugccacaa     2340

gaagacuuaa uaaugcaacu accagacagg ccgcaagcca aucaugaauu uguacaacca     2400

cuauuuguuu cucgugcccc aucaagaaaa auguccgguc agggccauga agcaaaguug     2460

agaucagcua gaaugcugga agccaccggc aaaucaauca aaaaagaauu uuuaaccgau     2520

cugaccauaa aaaaucuaga acacaugguc aacaaagaaa gagaaccaga auuauaccaa     2580

gcauuagucg aacacuucaa aaaauacgga gauaaaccua aagaaaaauu uuuuaaaaag     2640

gggggaguag aaguaaaauc aauaagaaua ucaaaaaccc aaaauaaauc cguaaaucua     2700

ggaaauaaaa cuauugcaga uaauggcgac auaguacgca cagacuuauu uuuaaagaac     2760

aaaaaguauu acuucguaaa cauauaugca uggcaaguuu caaaaggcau acucccuaag     2820

gaaacaacaa ccggaaacau acuggacaac aguuacgagu uucaauuuuc guuauuuaaa     2880

aacgacuuau uagaaauacc ccauccaaaa aacgagaacg auaguauacu ugccuacuuu     2940

auacgaccag acgacgaacg aagauggauc cuuaaauucc augauaacgc aaagauaccc     3000

gauauauaug guaaaaaaga cgaaacuuca auuagacucu ccauucaagg acaaaaguuc     3060

auaaaaaaau accaaguaga ugaacucgga aagaauauua gaccaugccg accuacuaag     3120

agacaaggag uacgauga                                                   3138


<210>  4944
<211>  3141
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4944
augaaaaucu acaguuucga cauaggagug gcaagcauug gaugggcauu cauagaaaac       60

gaagucuuac aagauugugg cauacgaauc uucacaaaag cagaaaaccc caaaaacgga      120

gcaucucuag cacuaccacg aagagaagcu agaggaguac guaaacgauu agcgcgaaga      180

aggggacgcc uaaacacaau aaaacaacua cuguguaaag aauuuggauu acagcuagaa      240

gacuacuuaa guaacgaugg agaacuucca aaggcuuaua cggcaucaaa agcaaauccc      300

uuaaaaucac cauacgaacu acguacacua gcccuaagcc aaaaacucga cuccaaagau      360

uuaguaagag ucauccuuca uauagccaaa cacagagggu acggaaauaa acacgccaaa      420

gacucaaaag acacugaauc aggaaaaguc aagaaagcca uagaauuuaa cagaaaaaca      480

cuugcagaaa aagguuaucg uucaguuggu gaauauuuau acagagaguu cuuucaacaa      540

agcagaauau cacaaaacaa uggcgcaacu gaguuuguca acguaagaaa uaaaacaggc      600

aauuacgaac acuguguagc ucaagacaug uugaaagaug aacuagaauu aauauuccgc      660

aaacagaaag aguuaggauu acaccuauca gaugguuuug aaaaacaauu acuugacaaa      720

auauucgaac aacgaccccu aaaguccuuc gcugauaaag uaggaaaaug ccaauucauu      780

gccggugcaa aacgagcucc caaagacuca auuagcgcca uagaauuugu ugcacuauca      840

aggauaauaa auacacuagc aaacuuauca aagaaaucgg gagagaugua cgauaaagca      900

augguacuaa caauccuaga uucuguacua gaaaaaggcg agaugaguua cuaugcccug      960

cgcgaagcaa uuaaccuaga cgagaaaaua agauucgccg acucaagacu ugauuacucc     1020

aaagaaauaa aagaaacaga aaaagugaaa uucguagaau gcaaaaaucu aaaaucuuuc     1080

aagaaaguau uaggaaauug cuacaaaacc cuggagcgaa aacauauaga cuccauugca     1140

gaacgcauag cgguaauaaa agaugucgaa aaacuucaca aagaacuaga aagcuauugu     1200

gcuacugaac aauuaucacu cacaucggaa caaauccaag cacuauccca uuuaaacuuu     1260

ucuucacaua uaucauuaag ccucaaagca uuagaacaaa uccuuccauu caugagaggc     1320

gaacgagaag caagaagcuc agaugcagac uauugcaucg gaaucgacga aucuggugaa     1380

agccaaugcc ugagauauga cgaaucagua gaaaaagccg ggcucaaggc uuuaucaaaa     1440

gacacgcuaa aaggacaaau auuacccccu cuuaaugagu ucgaacccua ccuagcaaau     1500

ccaguaguag caagagcucu agccgaauau agaaaagucu uaaaugcacu acucaaacaa     1560

uacggcucac cacacaaaau acauauagaa uacgcaagag aggccaaacu aaacacgaca     1620

gaaagacaaa aauacgaaaa agaacaaaaa gacaacuacg ccgcaaacca agcagcacau     1680

aagaaaugcc aagaacuagg cuuggaaccc ucuagcacua auuuacuaaa acuuaaacug     1740

uggcaagagc agggaggcuu auguuuauac aguggugcua aaauuacaau aagcuaccua     1800

caagacccca cagcacuaca aguagaccac auauauccau acucgcgauc auucgacgau     1860

agcuauauga auaaaugccu aguucuaacu aaagcuaacc aagaaaaagg caauagaaca     1920

ccuuuugaag cauuuggcac agauagcgca aaauggggug uaauuacaac cuuagcacaa     1980

aaauuacccc auaagaaaag aagaagaaua uuaaauacag cauuuaauga caaagaagca     2040

ggcuuuaaau uaagaaaccu aaacgauacu ucuuauauug caagacuugu agcugacuau     2100

acagaaaccu acuuagaauu ucucucauug guagaaucag agaacacaac auugggaaaa     2160

ggacaaaaag gaagcaagaa acacaucgca auagucaacg gcgcccuaac aucaacuaug     2220

agauacuacc uagguuucgc aucaaaagau cgggacaacc aucugcacca cgcauuagau     2280

gcagucauaa uagguuucau gaaugacucu gugauuaaag cauucuccga cuucaagaaa     2340

acucaagaaa caucaaaagc agcauacuau gcacacacau uguccaaaca agaauacaag     2400

aaacaaagag cuuuuaucca acuccccuca ggggaaaacu uccgagccaa cguacuagag     2460

aaagcugaau cagucuucgu aaguaaacca ccacgaaaaa ggguaagagg cgcacuacac     2520

gaggagagcu uuuacucuca aaacgacaca aaacuucuca aaaguuacgg cggugcagaa     2580

gguauugaaa aagcauuaca acuaggcaaa auaagacaaa uagguacaaa aauaguaucg     2640

aacggagcaa ugguacgcgu cgauauuuuc aaacaucgaa aaagcgggaa auucuacgga     2700

guacccguau acacaaugga uuucgcauua gggauacuac cuaacaaagc uguuguccaa     2760

ggcaaagaca aaaacgacgu aauuaaagau uggcuagaaa uggauucuuc cuaugaauuu     2820

auauuuaguc uauacaaaga cgaccuacua uuaguacaaa agaaagaaau ggcagaacca     2880

gaacuuuguu acuuugucuc auucgauaca ucuggggcuu caauuaaagu ugcaaaacac     2940

gacaacaauu uuagcacuuu aaccgaaaau caaaaauuac uuuucucaaa ugcaacaaaa     3000

gaagaaguag uaggaaaauc aauagguauu caaaauuuaa aaguuuuuga aaaauggcaa     3060

guaucaguac ucggagaagu uaaaagaacu caaaguuauc cacgagaaaa cauaucacua     3120

gcaucaaaag cuaagaaaug a                                               3141


<210>  4945
<211>  3144
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4945
augaaacucu uacgauauau auuaggacua gaccuaggaa uagcaucugu agggugggca       60

auuguagaaa uagacgagaa ugauuuucca auaagauuaa uagauauagg cgucagaacu      120

uuugauaaag cugaaguauc caaaacugga gaaucauugg cuuuggcaag aagauuagcc      180

agaaguacaa gaagauuaau acgaagacga gcauuuagac uucucaaagg uaagagacua      240

uuaaaacaac acaacguaau aaauaaggag gcauuaacca cacuaccaac ugauuguugg      300

gccuugaggg uaaaaggacu ugacgaaaga uuaaguccaa ccgaauggag ugcgguccua      360

uuacaucuuc ugaaacaucg aggauaccug ucacaaagga aaaacgaagc caaaagugca      420

gacaaagaga ugggagcccu ccuuagugga guagccucaa accaucaacu ccuaguggaa      480

aaccaauuua gaacgccagc agaaauagcu guaaagaaau uugccuuaga agaaggucau      540

auaagaaauc aauugggagc uuauacacau acauuuaaua gacuugacuu acuggcagaa      600

augaaacuac uauuucaaac acaacgagaa cuuggaaacc cccauacaag uggccaauua      660

gaacaggaau uuugugaacu cuuaaugugg cagaaaccgg cauugagugg agacgcuauu      720

uugaagaugc uagguaaaug cacuuuugaa acuaaugaau auaaagcagc caaacacucc      780

uacacagcag aacguuuugu auggcuaacu aaauuaaaca aucuuagaau acucgaacau      840

ggaucagaaa gaguacuaaa cacagaugaa cgaagucuau uauuaaacca accauacgaa      900

aaagcgaaac uaacauauac acaaguuaga aaacuacucg gacuaucaga agaagcuaga      960

uuuaauggug ugagauacgg uaaagaaaaa gcagaagaug caaccuuaau cgaacuaaag     1020

gcuuaucacg cuauaagaaa agccgucgag aaagcagaca aaagcgcaug ggaaacuuug     1080

aaaaaucaaa ccgcauuauu agacgaaauc gguacgacau uuucaauaua uaaaacagau     1140

gacgauauca cugagcaguu aaauggaaaa uugagcgaca caguacuaaa cgcuuuauua     1200

gagaacuuaa auuuugauaa auucauacaa uuaagccuaa aaaguuuaua uaacauugua     1260

ccuuuaaugg aacaaggccu aagauacgac gaagcauguc aacaaauaua cggagaccac     1320

uuuggaaaga aaucuggaga aauaaacagg uuacuaccac cgauuccagc agaugagaua     1380

cgaaauccag uaguucuacg aucucuaacu caagccagaa aaguaauaaa ugcggucaua     1440

agauuauacg ggagcccugc aagaauacac auagagacgg cuagagaacu agguaaaagu     1500

uacaaagaua gaaaagaaau cgagaaaaga caagaacaaa auagagcuga acgcgaaaaa     1560

gcgaccgcuc aauucaaaac acucuucccu acuuuuauag gggaaccaaa agguaaagau     1620

auuuugauga ugcgacucua cgaacaacaa aacggacaau gccuauacag uggaaaacca     1680

cuaaaccuaa aucgucuuuu agagaaagga uauguagaaa ucgaccacag cuuaccguuc     1740

ucaagaacau gggaugacag ucaaaauaau aaaguucuug ugcuagcagg cgaaaaccaa     1800

aauaaaggaa aucaaacacc uuucgaaugg cuaggaaaag augaacauca augggcacua     1860

uucguagcac gaguaaacgg uugucgauuu cccuacgcaa agaaacaacg aauauuaaca     1920

caaaaacuag acgaacaggg uuuuauugaa agaaaucuaa augauacuag auauauugca     1980

cgcuuucuag uaaauuuugu acgagaaaac uuacauuuaa cugggaaagg caaaaauagg     2040

guauucgcaa gcaauggaca gauaacagca uuucuaagag gaagaugggg acuuagcaaa     2100

guaagagagg aaucggaccg ucaccaugca cuagacgcgg uaguaguagc uuguucaacc     2160

gucgcaaugc aacagaaaau uacacgguuu gucaaauauc aagaaggaaa ccuacaaaca     2220

gcagaaagaa uagacagaga aacaggagaa auaauaccau ugcauuuucc cgacccuugg     2280

cauuucuuuc gacaagaagu uaugauccga aucuuuagug aaaacccaca agaagaucua     2340

auaauccaau uaccggaucg uccagaagcc aaucacgagu ucguacaacc uuuguuuguc     2400

ucaaggaugc caacacgaaa aaugagcggu caaggacaua uggaaaccau aaaaagugcc     2460

aaacgacuug augaaggaau uucagucucc aaaguaccac uaacacaacu aaaacuaaaa     2520

gacuuagaca auauggucaa uagagagcgu gaaaaagcau uauacgaggc uuuaaaagca     2580

aggcucaaug ccuuuaauga ugacccaacu aaagcauuug cagaaccuuu cuauaagaaa     2640

gguggacaac aaguaaaagc aguuagagua gaacaaguac aaaaauccgg uguaguugua     2700

agaaacggaa auggaguagc agauaaugca ucaaugguga gagucgaugu auucauaaaa     2760

gggggaaaau auuucuuagu accuaucuac accuggcaag ucgcaaaagg aauauuacca     2820

gaucgggccg uuguugcugc uaaagaugaa ucugaauggg aacucaugga caaaacagca     2880

caauuccuuu ucaguauguu uccaaaugac cuaauucaag uuaaaacaaa gaaagaaaca     2940

uucuuuggcu acuauaaugg cuuagaccga gcaacaggug cgauaaaucu aaaagaaccu     3000

gauucaaaua uaaacaaagg caaaagugga cuccauagaa gcauaggagu caaaacagca     3060

cucucagucg agaaauacca aguugacgaa uuggguaaaa auauaagacc auguaggccc     3120

aguaagcgac aggcaguaag auga                                            3144


<210>  4946
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4946
augaaaagac uaggauuaga uauaggaaca aauucgauag gauggugucu gauagaagau       60

gauggagaug gcaaaggaag aaucaucgau auaggagcaa gaaucuucuc agacgguaga      120

gauccaaaau cuggagcauc uuuggcggua gacaggagag ccgcaagagc uaugagacga      180

agaagagaua gguaccuugg cagaagaaca gccuuccuaa aagcacuaaa aagauucgga      240

cugaugccug cagaugaaag cgcggccaag uuacuugcag acagagaccc auaugaauua      300

cgagcaagag cacuagauga gagacuagaa ccacacgaga ucgggcgagc auuauuucau      360

uugaaucaaa gaagaggcuu uaaaucaaau agaaaagcag agagaaaaac aaaagaugcu      420

gaagacggga aaauugcuaa uggagccaaa gcgcuggacc aggcaauggc agaaaguggc      480

gcugaaacau uaggacaauu ucuuucauca cgagauacaa aaagaguaag aaugggaagc      540

gaugcucaag gcuaugacuu uuauccgcaa agaaggcaug uagaauauga guucgacaca      600

auauggagcc gacagcaaca acaucaucca gcacugcuaa cagaagaagc ucggaagaaa      660

uuacacgaca uuuuguucuu ucaaaggccc cucaaagaac aggaaguagg uguauguacc      720

uuuguucaua acgaaugccg acuaccuaaa gcacauccac uauuccaaga aaggcgauua      780

uaugaagaag uuaaccaacu agaaauuaca acuccuggag ccccgaaucg gaaauuaaca      840

auggaugagc gagacgcccu uauauuagaa uuaagaagua gaaaagaaag auccuucgau      900

ucauuggcaa aaagacugaa auuagcaccu ggacagagcu uuaauaaagc uagugaaagu      960

cgaacccggc uucguggaga ugaaguauau gcaguaaugg cccauaagaa aagauucgga     1020

gauagguggc cucaucuaga uagagauaga caaugggcua uaauaaaacg aauaauagaa     1080

gaagaagacc cagacaaacu acaugcauuu cuuacaggag aauggggacu uacugacgaa     1140

caagcguuag caacagccag agcaccauua ccagaauuau augggcgccu aggaccaaca     1200

gcaacggcac gaauauuaga ggaauuaaag aaagauguua ucacuuauga ugaagcagua     1260

cgacgagcag gauggcauca cagugaugac agaacuggag aaaucuugcc acaacuaccu     1320

uauuauggag agcuauuaac uagagacaua ccaccgggaa cacaagaccc aaaugauccc     1380

ccggaacaga gauggggaaa aauaacgaau ccaaccguuc auauaggacu aagacaacua     1440

gaaaagcuag ugaaugcuau aauaaaagua cacggaagac cugaucaaau ugucguagaa     1500

cuugcuagag aauugaaacu aaaugagaaa gacaaagaag aacacaauag aagaauaaga     1560

agagacacug cagcagcgca auuaagguca gcaaaauuac agagugaagg cauacccgau     1620

acaggagcua auaggaugcu uuugcgaaua ugggaagaac uuaauccagg agaaguacug     1680

gauagacgau guccauauug uggagaacca guaggaauga gaauguuauu ugcuggggaa     1740

gcagauauag accacauaau uccauauucc aggucauuag acgacagugc aggaaauaaa     1800

guaguagcgc auaggaguug uaacagagca aaagggaaca gaacaccaua ugaaaaaugg     1860

guccaugauc cagacaggug ggauguaauc ucaacacagg uagcuagauu gcauaaauca     1920

aaacaaugga gauuuggccc agacgcuaug gcacgggucg aaaaagacgg uggauuuaua     1980

gcccgccaau uaacagauac acaauaucua uccagacuag caggacgaua uuuaagcuca     2040

cucuacccca cgaaagacga uggaucaguc uauguaauac caggacguau gacagcuaug     2100

cuaagaagac gaugggguuu aaauagcuua uuaccagauc auaauuucgu agacaaugaa     2160

cauucaaaug caccgaaaaa uagacuagac cauaggcacc augccauaga ugcagcugua     2220

guggcuguua cgacuagagc acuaaugcaa gaaauugcac gaacagccgg ccgagcggaa     2280

gauaaagacu uagaccgauu auuugaagau uuaccgcaac caugggaagg uuuuagggaa     2340

gaguuaggug aaaaauuagu cagaguaacu gucagccaua aaccggacca uggacgaaaa     2400

ggucuuccuc cuaaaaauag agauguaaca gcaggccgac uucacaauga uaccgcauau     2460

ggccucacug gugacguggc agcagacgga aaaacaccca uuguagucca ucggguccca     2520

cuuaugucgu uaaaaccggc agauauaucg gauccagacc gaauaccaga uccugcucua     2580

agaucggcac uccauagagc aacagaugga cugaguggaa aagcguauga ugccgcacua     2640

gcgggauucg caaaaacuca uccucaauuu gccggaauaa gacgaguacg aguuagagag     2700

ccacuaacug uaauccccau agcagauaga gaugggaggc cuuuuaaggc uuauaaagga     2760

gaugcaaaua gucgaguuga uauauggcga augccagacg gaaaauggcu aucugauguu     2820

gucacuaugu augaagcaca uagaagagau cauucagacc gucggccaca uccagcagcu     2880

aagaaauuaa ugucccuccg acaaaaugau augauagcag uagaaagaga cggggcucca     2940

cgacaaauca ugguagugaa acaaauaugg cccacacaaa uuaaucuagu agaacauaaa     3000

gaaacaggua acuuaaaaga uagacauagu aaugaaacug acccuuuccg cuuuuucgcg     3060

ccaacggccg gcggauuaaa gaaaaugaaa gcacgacaag uaagaauaga cccccuagga     3120

agaauauuug auccaggucc aagauga                                         3147


<210>  4947
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4947
auggcgaaca uagcugaaaa ucuuguauuu ggaauagacc uuggcauagg aagcuguggc       60

ugggcagugc uaaaucaaaa agcaaaagau gaaaacauag uugccuuagg aucguggugc      120

uuugacguac cagaaacuga uaaagaaaga auaccuacaa accagaucag acgugcaaac      180

agacucuuac ggagaguaau aagaagacga cggaauagaa uggcagaaau uaggcgguua      240

uucgcaucua agggaaugcu agauacagca gacccagaug cgcuagcaaa aauaucagcg      300

gaucccuggg aaaugagggc aaaaggacua gacuaucagu uaacaccgca agaauuugca      360

guugcauuag gacauauagc aaaaagaaga gguuucaaau cggcggcaaa aagaguaucg      420

accaaugcac caucagaaga uaguaaaaug cucgcugcgc uagaaaaaac aaaggagcaa      480

agugcccaau auagaacaau aggagaaaug uucgcuagag aucccgccua ugccgauaga      540

agaaggaacc gagauggaug uuucgacaga acuaugucua gagaagaucu auuuaaagaa      600

acacaaauac ucuucgaauc acaaagacgc cuaggaaauu uuagugcuac cucugaaacc      660

gaacaagcau uuauagauau agcauuuaga caaaaaccaa ugcaagauuc ugaaagacua      720

guugaaacuu gucuguucga accugaugaa aaaagagcuu caagauuugc accuucauuu      780

gaaagguuua gguuauugug uagacuagua aauuugagag uaacugaugg uuuggaagau      840

agaccacuaa caccggaaga auuaagacuu gcaugugaag augcugggaa aacacguaaa      900

uugucaguaa agaaaguacg aucuuuaaua ggacuacgag augaucaacg auucacuacu      960

auaccucccg aucaagaaca acacgauaua gcggcuagaa ccggcgacgc auuaacuggu     1020

acggccacau uccgagcaau ucuaggagaa aguuucugga gacaauuauu accacaccca     1080

gaacaacuug acagagcggc auggauaauu uccuuucaug aguuaacaga cacaauaauu     1140

agcaaguuag cgacgauugg acuaccuuca gaaguaaugg aacuuauaag aaaagcccua     1200

gaagacggga aguuuaguaa auuuaaagga gcggcaaguu uaucagauaa agccgcaaga     1260

aaauuaauac cguuccuaca agaagggcua acauaugaua aagcuuguca gaaggcagga     1320

uacaaccaug cagcaaguaa auuuacacac uacgauucaa uagauacaaa agcaaaauuu     1380

aauucucuaa uggacgaugu aagagacagc auuacaaacc caguggcaag aaaagcaaua     1440

agcgaaggua ugaaacaagu augggcuaug agaaaucaau ggggauuacc aggagccaua     1500

ugcauugaau uagcaagaga uguugggaau agugcagaaa aaaggcgaga aauugaaagu     1560

ggaaucaaga aaaacaccgc ucaaagagaa cgugagcgac aagaagcuaa agaacaccuu     1620

aaccagagug auauaagcag cgaaacucuu uugagauauc gacuauggaa ggaacagggu     1680

ggaagauguu guuacacgga ucaaccuaua acuccaucac aacuaauagc aagcgauaac     1740

ucuguacagg uagaccacau auuaccaugg ucaagauucg gagaugauag cuaccugaau     1800

aaagcacucu gcacagcaaa ggcaaaucaa gaaaaaaaaa acagaacucc uugggaaugg     1860

auccacaacc aaaaaggcgu agaagccugg aaucauuuua ccgcugcagu ggaaagccga     1920

aaagaaaccc gaggcuuaaa aaaaaggaau uuucuauuaa aaaguucaaa agaaacagaa     1980

gaaagguuca ggaguagaaa uuuaaacgau acacgauaug cagcccgggu aauggccgaa     2040

gcaguaaggu uacuguaucc aagugggaac agagcugaga agggaggugu gaaacgaguc     2100

uuuaccagac ccggagccuu aacugcagca uuacggcaug cuuggggggu agaaagcuua     2160

aaaaaaauaa augguaaaag guuaaacgac gacagacauc acgcucuaga cgcagcagug     2220

guggcggcgg uaucagaaaa agaaauacaa aaauugacaa aaucauucca aauaugcgaa     2280

caaaaagguu uagcgagacc caugcgagau guacccaugc caugggaagg auuccgacau     2340

caauuggaaa cggcauaugc agauauucga guagcacggc cagaaagaag aagagcaaga     2400

ggaaaaggac augacgccac uauacggcaa cuccgagaag aaaaagaugg accaguagug     2460

uaugaaagaa gagcaauaag ugacuuaaaa cuaacagauu uagagagaau uaaagaucca     2520

gagagaaacu cagccauaau aagcagcuua aggacgugga uagaaaaggg uaaaccagca     2580

gacuauccac cgcaaucucc gaaaggagau gaaauaagga aaauaagggu uaugaguaga     2640

aauaaaccgg cgguauccgu ucgaggagga accgcugaua ggggugaaau gacaagggug     2700

gacguauuua caaagauggg aaaaaaaggc aaaccagaau gguuccuagu accgauauau     2760

aggcaucaag uaauagaucg aauccaaugg ccucaaccac caaaccaagc agcagcaaau     2820

ggcaagagug aaaaaaacug gccuaccaug ggacuagaac augaauuucg auuuucauug     2880

uacccuagau cauaugucca aauaguaaaa agagacggcg aaauuauaga aggauauuuc     2940

gcaggacugg acagaacggu agcagcguuu agucuucuau ccaguucaga uccgacuuca     3000

auacaacgag guauaggagc aaagacauua gaaaguauac ggaaauacaa cguaaaccga     3060

uuuggagaua uaagcauggu ggaacaagaa acacgaacau ggcauggaga cguaugcauc     3120

agcccggauc aaccggauuc cgcauga                                         3147


<210>  4948
<211>  3147
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4948
auggucgaaa aaauacuugg uauagaccua gggauuagcu cacuuggaug ggcaguagua       60

gaauaugaua aagauaacga ugagaauaau aaaauaauag acuguggagu aagacuauuu      120

acagccgcgg aaaccccaaa agaaaaggag ucgccaaaua aagcacgacg ugaugccaga      180

ggucuaagga gaguuauaaa gagacguaga guuagaauga acacaauaaa aaacuuacua      240

auuaccuaua aauuaauaga caaaacccuc uuagaugagg aaaugggcau guuucauucc      300

caaagcaacc gaguggacgu auggaaacua cgccacgaug cgcuauauca ccucuuguca      360

ggugacgaac uagcuagagu ccuaauccac auagcaaaac acagaggaua caaguucuua      420

ggugacgacg aaagugacga agaaaguggu aaaguuaaaa aagcaggagc agaacugaaa      480

aaaaaauucu uagaagccgg augccagagu guaggugaau ggcuuuggaa agaaagagga      540

cuccaaggaa aaaaaaggaa caaaucagga gacuaugaaa uuuccauucc acgugacuuc      600

cuagucgaag aaauacaaag aauauuugaa acucaacaga aauucggauc uaccuucgca      660

acaagcgaau uacaaaaagc cuauacugac auagcauucu acguaaaacc aaugcaaagc      720

auugaagaca ugguaggaua uugcaccuuc uacccaaaaa uaaaaucuaa aaaucaagac      780

ggcgaaaaaa gagcacccaa agccucacca agcgcagaac aguuugugau auuaucaaaa      840

aucuuuucaa caauagugau agaugaaaau aaacaagaaa aaaaauuaau agaacuaaaa      900

aguauagaac agcugauaca aauugcccgu uccaaagaaa cacucaaaua caaacaacua      960

aggaaagaac uaaacuuagc aaaagacaua uccuuuaaaa guauaaguga ugaggaaaaa     1020

acauggauca accuaguagg caacgcaaag uucaaaaaaa uccuaggacu aaacuaugaa     1080

acauuccuua aaaacacaga aauaucagac gaaaucgcca aaauauuaac uuacgauaaa     1140

acauucgaac aaaaagaaac aaaacuuaaa aaccuacuag uaaacaucga cuggauagac     1200

aauaaccaca uagcagaauu agcaaaauua agcuucucac aauucaacca acuuuccuua     1260

aaagcuauaa aaaucauauc aaaaauaaug auagaaggau acgcuagaua ugacgaagca     1320

guacaauacg cauucgaaaa uaaucuacuc ccaaaaccuu cacaugaaaa aucaauacua     1380

uugccacccu uaaaagaaac aaacaucgca auauuaaacc cuacgguaau ccgugcauuu     1440

gcacaauucc gucaaguagc caaugcguua guuaguaaau acggaagcuu cgacaaagua     1500

cacuucgaac uagcuagaga agucaauacc aaagaagaca gaaaaagaug ggaaaaagac     1560

agagauaaaa augaaaaaau gcaccgacaa auaacagaaa agcucguuga agaaggggua     1620

aaaccgucau acaaaaauau acuaaaaagu aaauuaagaa gugaacaaaa agacaccugc     1680

cccuacugcc aaaaaaaucu acauuauccc augauauuug aagauggcua cgcagaaaua     1740

gaccauauuc uacccuuaag ucaaucccag gacgacucau acgucaauaa aguucuaguc     1800

cacucagcau gcaaccagaa caaaaaaaac agaacaccau ucgaaugguu ucaagaugaa     1860

aaaaaagauu gggauacauu caagaguuac aucuuaaugg aaagcacacu gggagaaaaa     1920

aaacgcaacu accuaaucaa agaaaauuuu ucagacccuc aaucacguaa agaauuuaua     1980

agccgaaauc ucaaugacac acgcuacaug ucuaaagcaa uaaagaccua cugcgaaaac     2040

cauuggaaac uaagccauga cgacgacaaa cuccgcauac aaguacgaag uggaaaacua     2100

acaagcacac uaaggcauca auggggacua gacaacaaaa acagagaaac ucacacacau     2160

cacgcaaugg augcaauuau gauagccuuc ucaacucaag gaaugguaaa aaaacucagu     2220

gauuauuuug caaaaaaaga agcgaaaguu gaaaaagaua aaccaguacu aauaacccca     2280

auaaaacaau ucaaagaagc aguagaacaa gcuacuacuu uagaacguca agaaaguaua     2340

caaacaaaag cuggagacac aauaacacuc aaccgacuac uaauaucacg accuccucgu     2400

gcuuccguua caggagccgc acaugaacaa acugccaagc ccuacccuag aauaaaacca     2460

aucaaaaaua aauauaaaag gagaagaaua ccaaucgaug aagauaaauu cgaauuauuu     2520

aggaaugaua aaguagcaag uggaaaugac aagaacuuuu acaacagcag cacaauacca     2580

agaguagaua uauacaaaaa agacgauaaa uaucacguug ucccaauaua ucuaagugau     2640

augaccaaag cugaaguacc aaacaaaucg cuaggaacaa acccugaagg cauggaugaa     2700

aaauacuucu guuucuccgu cuuuaaaaau gaccuaauag aauuagaaac uaaagcaacu     2760

cccaaaaaac caucuaaaaa acucuuaggu uacuucaaac aacucaacgg agcuaacuuc     2820

auacuaaacu caauucauaa ugguauaauc gacggcuucg uauguagccc cauaacacua     2880

uuuaaacaac agaaagacau gugcaaaaaa ugucuccccg aagacagagc cauaggcaac     2940

uguucccagg aaacauuaga auucugggaa gcagaaaaca uaaaaguacc aaaaaaagau     3000

uuugaaugcg accaagguau caaauuugcu auugcuguua gaaaauacac cauagaccca     3060

cuaggauacu aucaugaagu aaaaggcgaa aaguuauuag gaacaauacc acagggggca     3120

aaaaaacauc caaaacgaca gaaauga                                         3147


<210>  4949
<211>  3150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4949
augauaaaug aaagauugac uuuuggcaua gaccuuggca uugggagcug cggaugggca       60

guccuaagac acgcugaugc agcuggggag ccaggaguua uagaaggacu gggcucaugg      120

uguuucgaug uaccagaaac aaguaaagaa agaauaccaa cuaaucaagu gagaagauca      180

aaucgauuau uaagacgggu aauaaggaga aggagaaauc gaauggcaga aauaagaagg      240

cuauuucacg cacagggauu acuuccgagu gcagaugaag augcauuaaa acguccgaga      300

uuagacccau gggaguuaag ggcucgcgga cuggaccagu guuuaagugc ugaagaauuu      360

gcuguggcac ucggccauau ugcaaggcau cgaggauuca gaucaucugc uaaacguaaa      420

gcugcaaaua caagugggga agacucuaaa augcuaucug cucuacuugu uacgagagag      480

aggacugcua gauaucgaac uguaggggaa auguuugcga gagauccaga auacagugcg      540

cgaagaagga acagagacgg uauuuucgau agaacuguag caagagauga uuuaauacau      600

gaaguaggaa aauuauuuga ggcacaaaga gcaaggggaa gugcauucgc uagcccugac      660

uuggaagcug auuuuaacgc aauugcuuuc agacaggcug aaaugcaaga cucggaaaga      720

cuuguuggaa acugucccuu ugaaagagau gaaaaacgag cugcucgaug ggccccauca      780

uuugaaaggu ucagauuauu aacgagauua auaaacuuga gaauagcaac agcagaagga      840

gaacggagau uuaccccaga ggauuuggca aggguaucag ccgaacuagg agcuacagca      900

aaauuaccug uaaguagagu aagggcauua auaggauuac uaccugauga acgauucaca      960

acaauuagag uagaagauga ggauagagau auuguagcaa gaacaggaga agcagcacca     1020

ggaacuugga gacucagaaa aacauuaggu gaagcucucu gggcagagau gcaggcacga     1080

ccugaucaau uagacgcuau agcacacguu uuaaguuucu ucgaagcuac agcaaaaaua     1140

agguccgaac uggauaggcu uggccugcca gcagcaguua uugaagcacu cgaucgagga     1200

cuggaagaug gagcauuugc uaaauuuaag ggagcugguc auauuucagc uaaggcaugu     1260

cguaacuuac ugccucauuu acaacaagga cauagauacg accaggcaug uagacucgcc     1320

gguuaugauc augccgccag uacucuauca cauacagcac aaguaguuuc caaggcagga     1380

uuuaaugcgu uaguuacgga acuaggagaa ucaaucgcua auccaauagc uagaaaagca     1440

uuaacagaag guuugaaaca aauuagagcu augugcgcaa gauggggauu acccggagcg     1500

auacacaucg aacuagcuag agaaguaggu aauagcauag agaaaagaag ggaacuagaa     1560

agucaaagag aaacaacaac aaaaagaaga caaacagaaa gagaugaaau aagaggacug     1620

uuagccuuag aagauguaga ugaugauaca uuacucaggu auagacuaug gaaagaacaa     1680

ggaggaaaau gucucuauac aggaaaagau auccacguga gacaauuaau agcuacagau     1740

aauucugucc aaguugauca uauuuuacca ugguccagau ucggagauga uagcuuuaau     1800

aauaaaacac uaugcuuagc gagugcaaac caaagaaaaa gaggacaaac accaugggaa     1860

ugguuuaguu uaacagguac accagaagca ugggaaguuu ucgcuucccg uguugaaaca     1920

aguaaagauu uaaggggauu uaagaaaagg aauuugcuuc ucaaaaaugc agaugaaguu     1980

gcucaacggu uuagaaguag aaacuuaaau gacacaagau augccgcuag auuauuagca     2040

gaagcggcaa aacuguucua uccagcagga gauaggcaag aaaaagaugg acguagaaga     2100

guuuugacga gaccuggacc auugacagca gcuuuaagac acgccugggg auuagagucc     2160

uuaaagaaag cugaugguaa acguguaucc gaugauaggc aucaugccuu agaugcgcua     2220

gcuguagcag ccauuagcga aagagaagua caaagauuaa caaggaguuu ccaagaaugg     2280

gaacaacaag ggcuagcucg accacuaagg cacguggcac caccauggcc uggauuucgg     2340

ggagauguac uagcugcuua cgcucaggcg uucgucgcaa gaccagaaag aagacgagcu     2400

agaggaaaag gacaugaugc aagcauacgc caaguaguag gagaaggucc agaagcacaa     2460

guauucgaga gacggggauu ggcugaucua aaagaagcug aucuagacag aguaaaagau     2520

ccugagagaa aucaagcuau aauagaagca guuagagcau ggauauuagc aggaagacca     2580

gcagaugcac caccaagauc cccaaggggu gaugucauug ugaaauuaag aacugcuccu     2640

gcgaagaaaa ucaaaccggc aguaccaguc cgaggaggaa cggcagacag gggagacaug     2700

guacguauag auguauuuac aaggccaaac agaaaaggca gagacgaaug guaucuaguu     2760

ccaauauauc cacaucaggu aaugaauaga aaagcauggc cacacccgcc uaugagaagu     2820

guaguagcau acaaagauga agcagguugg acagagauag gagcggaaca cucauucagg     2880

uuuagcuugu auccgagauc auauguacag uuggucaaaa guaacggaga uguaauagaa     2940

ggauauuuag ucaguaugga caggaguacu ggggcauuca gaguguuugu gcauaauaau     3000

ccagcugaga uggcaaaagg aauaggugca aaaacauuau uaaaucuuag aaaauuuaac     3060

guggacaggu uuggcgugag gacggaagua aaaagugagg uacgaacgug gcauggagag     3120

guaugcaccu caccuguacc accagacuga                                      3150


<210>  4950
<211>  3150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4950
augcaacuau caaaacuaaa cuacauuuua ggcuuagauc uaggaauagc uucaguaggu       60

uggucuguga ucgaaauaga ugaaacugaa augccaauac gauuaaucga uguaggagua      120

agaacauuug aacgagcaga aguacccaaa acuggugaau cucuggcacu agcuaggcga      180

uuagcaagau cuacaagaag acuuauaaga aggagagcau uuaggcucuu aaaagcaaaa      240

aggcuacuaa agaaucauca aauaguaagu gccgacgaau uaagcacccu gccaaaucac      300

uguugggaau uaagaguaaa aggucuagac gaacgacuca caugucagga augggcagca      360

guacuucucc auuuauuaaa acacagaggg uauuugagcc aaagaaaguc ggaagcacaa      420

aaugcagaca aagaaauggg agcucuacuu uccggaguag caaacaacca caaacuacuu      480

ucagaaaacc acuaccgaac acccgcagaa auagccguaa agaaauuugc cuuagaagag      540

ggacacauga gaaaucaaag aggagcuuau acucauacau uuaacagacu cgacuuauug      600

gcagaaaugc guuuacuauu ucaaacucag agagaacuag guucaccuua cgcauccgga      660

gaacuagaac aagaauuuug caaacuacug auguggcaaa aaccagcacu uaguggcgac      720

gcaauacuaa aaaugcuagg aaaauguacu cuagaaccca cagaauauaa agcagcuaaa      780

aauacauaua cagcagaaag auucguaugg cucacuaaau uaaacaauuu aagaauccua      840

gagaaugguu cagaacgagc auuaaaugca gaugaaaggg cacuauuacu aaaccaacca      900

uacgaaaaag caaaauuuac guaugaacaa gcaaggaaac uuuuacagcu aaaugaggca      960

gcgagauuua auagcguucg auauggaaaa gaaaaugcag agaaaagcac uuuaauggaa     1020

augaaagccu aucaugcaau ucguaaagca guggaaacag cagacucauc agccugggca     1080

acacuaaaaa cacaaccacu ucuuuuagac gaaauaggaa cugcauuuuc aauauauaaa     1140

acagaugaag auauaaaaac acaacuacaa ggcaaauuag cagacaaugu aauagauguc     1200

uuacuagaaa auuuaaauuu ugauaaauuu auacaauuau cauuaaaagc acucuacaaa     1260

auauuacccc uuauggaaca aggacuacgc uacgacgaag cauguucaca aauuuauggu     1320

gaucauuucg gcaagaaaga acagaaaaau gauagacuau uaccccaaau uccugcagau     1380

gaaauaagaa acccaguugu auuacgaacg cuaucacaag cccgaaaagu aauaaaugcc     1440

guaauaaggc ucuacggaag cccagcaaga auacacauag aaacagcaag agaacugggc     1500

aaauccuaua aagacaggaa agaaauagag aaaagacaag aacaaaaucg agccgaaagg     1560

gaaaaagcag uagacaaauu caaagaauua uuucccaacu uuaucggaga accaaaagcc     1620

aaagacaucc uaacuaugcg uuuauaugaa caacaaaacg gacaaugcuu auauucaggg     1680

aaaagucuag aucuacaccg uuuauuggaa aaagguuacg uagaaauaga ucacgcucuc     1740

ccuuuuucaa gaacauggga cgacucauuu aacaauaaag uucucguauu ggcuggugaa     1800

aaccaaaaca aaucuaacca aacaccauuc gaauggcuag guaaagauga acaaaaaugg     1860

gcacuauuua cagcuagagu caacagcugc agauuuccgu auuccaagaa acaacgaaua     1920

cuaacaaaga aauuagacga ugaagguuuu auacaaagaa accuaaacga uacuagauac     1980

auagcaaggu uccucguaaa cuuuguuaau gacaaucuac auuuaaccgg aaaaggaaaa     2040

agacgugugu uugccucaaa uggacaaaua accgccuucc ugcgagguag auggggacua     2100

acaaaagugc gagaagaaag cgacagacau cacgcacuag acgcaguagu gguagcaugu     2160

aguacaguuu cuauacagca gaaaaucacu agguucguga aauaucaaga agguaaccua     2220

caaacagcag aaagaauaga ucgagaaaca ggagaaauca uuccauugca uuuuccugau     2280

ccaugggcau ucuuuagaga agaaguuaug auuagaauau uuagugaaau gccacaggaa     2340

gaccuaguaa uaaaacuacc agaucgucca caggcaaacc acgaauuugu acaaccacuu     2400

uuugucagca gaaugccuac ucgaaaaaug ucaggucaag gccauaugga aacaaucaaa     2460

ucggccaaaa gacucgauga agguauaagu gugucaaaag uaccauuaac acaauuaaaa     2520

cuaaaagacc uagaaaauau gguuaaucgc gauagagaaa uugcucucua ugaagcacuc     2580

aaagcgagac ucacagaguu uaaagaugac ccugcgaagg ccuuugcgga accauucuau     2640

aagaaaggag gaguacaagu aaaaucaguc cgaguugaac agguccaaaa guccggaguu     2700

guagucagaa acgggaaugg cguagcagau aaugcaucaa uggucagagu ugaugucuuu     2760

auaaaaggug gaaaauauuu ccuuguccca auauacacgu ggcaaguugc aaaagguauu     2820

cucccggaca gagcaguaau agcauauaca ccagagaacg aaugggaacu aauggacgau     2880

ucagcccaau uucaauuuuc aaucuaccca aaugauuuaa ucaaauugac uaccaagaaa     2940

gccaucuuuu ucggauauua uucaggauuu aacaggcuaa caggcgcaau uaacaucaaa     3000

gaacacgauc uccaaauauc aaaaggggac aaaggaguuc aucucgguuu aggcguaaaa     3060

cuagcacuaa gccuugaaaa auaccaaguc gaugaacuag gcaaaaacau aagaccaugc     3120

agacccacac aaaggcaaaa cguaagauga                                      3150


<210>  4951
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4951
augaguuccu auagauuuuc auuugacuua ggaaccaauu caaucggaug ggcuguauuc       60

gagcuggacc cuaaaacgcg accaguagcu uuagcagauu guggcguccg aaucuuucca      120

gacggaagaa acccaaaaga uaaacagucc uuggcaguag caagaagaga ggcccgagcc      180

guuagacgac gaagagaucg uuauuuaaaa agaaggucag aauuaaugga aagauuagua      240

gcaaaaggac uaaugccaga aaguagagac gaucaaaaag cauuagaagg acucgauccc      300

uaugcacuac gagcugcagg auuagacgac gcauuaccac cacaccacuu uggcagagcc      360

uuauuucauc uaaaucaaag aagaggauuu aaaucuaaua gaaaaaccga uaggggagaa      420

aacagugaaa caggaauaac aaaaauagga augaagaaau uacaugaggc ucuacucagu      480

gaaggaugua ggacacucgg agaauuacaa cauaggcauc acgaagcuaa ccaaaccccu      540

gcaauggaca caaaucccaa aucaguuaga aguaaauuac gcggugaggg ggcaaaagcu      600

guauaugauu ucuauccauc aagggacgca guagaagaug aauuuaacaa acuaugggac      660

agucagaaaa gguuucaccc agauuugcua acagacucag cacgagaugc uauuagagau      720

gcaauauucu aucaaagaaa auuaaaaaca ccugacgcug gaagaugcuc auuuaauccu      780

gcggaagaga gaguagcaag agcaaugccc auagcacaac acuuccgaau acuacaauuu      840

guaggcgauc ugagauuaau agauaacgau cuaguacgac auccguugaa caaugaucaa      900

aagaaagcuc ucuacagaga uuuacuagca aaagguaaau ugacauuuaa agcaaugagg      960

aaaacaauag guaucggcuc agaguacgcc uuuucucaug aaagugaaaa acaaucugag     1020

uuuagaggag augaaacagc agcaagacug gcuggaccua aauacuuugg accgaagugg     1080

agagaacuau ccuucaacga ccaaucugaa auaguagaca gguuauuaca ugaagaaaac     1140

gaagaaacuu ugauacaaua ucuucaagau cgauuuggau uugaucauga ucgaugcgaa     1200

guaauagcug acgcacccuu gcccgacgga cacaccagac ucggaaaaac ugcucuagaa     1260

acauuaguag aaauaaugag agacgaucac uugcccuaug aucaagcaug caccaaagca     1320

ggauacgaac augcacuacc accugaugga guaauacuug augaucuacc auauuauggc     1380

aucgcauuag aacgacacgu agcauuugga gauggaaaaa gugagaaucc agagaagagg     1440

uauggaaaga uagcaaaucc uacaguacac auagcuuuaa accaacuacg uaagguaauc     1500

aaugcuuuga uugcaagaua uggacacccu gaucaagugg uaauggaaau ugccagagau     1560

uuaaaacagu cuaaagcaaa aagagaugau auaagcaaag aacagaagaa aaaccaagac     1620

cgaaaugauc agcgccgcaa gaaacuucaa gaggcccaua uagcaguuaa cgcagaaaau     1680

auguuacgua ugagacuuug ggaagaacug gacgaggaua acgcugcagc aagagcuugu     1740

ccauuuacug gugaaacaau cucaauggca cgauuauuuu cacccgaagu ggaaauagaa     1800

cauauuuuac cuuuuucacg aucacuggau aacaguccgg caaauaaaac ucuaugcaug     1860

guaugggcaa acagagcaaa agguaaucgc acuccauggg aagcauuugg aagagauaga     1920

gaaccagggu uugauuggcc ugaaauugcg gcacgagcag cuagauuacc caaaaacaaa     1980

aguuggagau uuggcccaga ugcuauggaa cgauuggaga gagaaggaga cuuucucgau     2040

aggcagcuaa cagacaccca auauuuguca agaauagcga gagaauacgu aacaaaaaua     2100

ugugauccaa aucaaguuug ggucacacca ggaagauuaa cagccugguu aagaaggaac     2160

uggggauuaa auggaaucuu accaacugga aaugcaacua aaaauaggga cgaucacaga     2220

caccacgcaa uagaugccau aguagugggg uuaacggaua gaggaauacu aaauagagua     2280

agcaggucag cagccuuagc cgaagcagaa gauuuagaca gaagcaucgc agaaaugcca     2340

gauccauuug acggauuccg ggauaauaua cgacacauau uagaacaaau uaucguuagc     2400

cauaaacccg augcaggauu uguaccugga caucccaagg gaaauacgac accaggagca     2460

cuccauaaug auacagcuua uggaauuaua aaaacagaug ccaaagguau gucagaaguu     2520

gucacaagaa aagacguuuu gucuuucaaa gggacaaagg aaauagaaag uauaagagac     2580

gcagcaacgc gacaaagccu auuagaugca gucgcaggag uccccagcgg uuuagcauuu     2640

aaggaagcau uaagggacug gucaguaaga acagguacaa aacgaguucg aguaacauuc     2700

aauaugucag ugauaccaau acaggaucga gauggaaaag cguauaaagg auacaaagga     2760

gauggcaacc uaugcgcaga aauuuaugaa cgaccugacg gaaaaugggg ugacgagaua     2820

aucucaagau uugaugcagu ucaaccgggc uuuacacccc aauggcaacg agaucacccu     2880

gcggcacauc gcguaaugag acuguucagg gaugacauga uaauggcgga gauagaugga     2940

guuaugacgg uauguaggau aguaaaaaug aguccuggaa aaguaguauu cacaccacau     3000

cacgaagcaa auaccgauag uaggaaucga gacaaagaug acccuuuuuc auuccauacc     3060

ugcuccggag aaaguuuacg caaaagaaaa gcacaaaaac uauuuguaga uccacucgga     3120

aagauaagag guuauagagc aaaagaaggg uga                                  3153


<210>  4952
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4952
augggugaaa auaugauaga cgaaucacuu acauucggua uagauuuagg uauaggaagu       60

ugugguuggg cgguacugcg ucgaccauca gcauucgggc gaaaaggggu aaucgaagga      120

augggaucuu ggugcuuuga ugucccagaa acuagcaaag aaaggacacc aacgaaccaa      180

auacgaagau caaaucgguu auuaagacgg guaauaagaa gaagaagaaa ucguauggca      240

gcaauacgaa ggcuauuaca cgcugccgga uuauuaccga guacagauuc agaugcuuua      300

aagagaccag gucacgaucc augggaauua agagcaagag gacuggauaa accuuuaaaa      360

ccugucgaau uugcaguagu acuagggcac aucgcaaaac gaagaggcuu uaaaucggcu      420

gcgaaaagaa aagcaacaaa uauaaguuca gacgauaaga aaauguuaac agcacuugaa      480

gcuacaagag agagacuggg aagauauagg acagucggug aaauguuugc aagggaucca      540

gauuuugcca gcaggcgaag aaacagagaa gguaaauacg acagaacaac agcacgagau      600

gaucucgaac acgaggugca ugcacuauuu gcagcacaac gaagauuagg gcagggauuc      660

gcgagcccag aacucgaaga agcauucaca gccucagcau uucaucaacg uccgaugcaa      720

gauucagagc gauuaguagg auuuugucca uuugaaagaa cagagaaaag agcagcaaaa      780

uugacucccu cguuugaaag auucagacuu cuugcaagau uauuaaacuu aagaauaacu      840

acaccagacg gagaaagacc ccuaacugua gaugaaauag cgcuaguaac cagggaucuc      900

gguaaaacag caaaacuaag uauaaaacga gugagaacuu uaauaggcuu agaagacaau      960

caacgauuua cuacaauaag accagaagau gaagaucgag auaucguagc gagaacgggu     1020

ggggcgauga cgggaacagc uacccuaaga aaggcuuuag gugaagcauu guggaccgac     1080

augcaggaac ggccagaaca auuggacgca auuguucaag ugcuaucauu cuucgaagcc     1140

aaugaaacaa uaaccgaaaa auugcgagag auaggauuaa cuuuggcugu acuagacguu     1200

uuacucacug cucucgaugc uggcguguuc gcaaaauuca aaggugcugc acauauauca     1260

acuaaagcug ccaggaaccu acuuccacac uuagagcaag gaagacgaua ugaugaggca     1320

uguacaaugg ccggguauga ccaugcagca ucuagguuaa gccaucacgg ucaaaucgua     1380

gccaaaacac aauuuaacgc gcuaguaaca gaaauaggag aaaguauugc uaacccaauu     1440

gcaagaaaag cacuaauuga aggauuaaaa caaauauggg cuaugagaaa ucauugggga     1500

uuaccuggau caauacacgu cgaauuagcu agagacguag gcaauuccau agaaaaacga     1560

cgagaaauag aaaaacacau agagaaaaac acagcauuaa gagccagaga aagaagagaa     1620

guacaugacu uauuagacuu agaagaugua aauggagaua cacuauuaag auauagauug     1680

uggaaagaac aaggaggaaa augccuuuac accgguaaag caauacauau acgacaaaua     1740

gcagcuacag acaauucugu acaaguggau cacauucucc cauggaguag guucggugau     1800

gauuccuuca acaauaagac ucucuguuua gcauccgcca aucaacagaa aaagcgaagu     1860

acaccuuaug aaugguuauc aggacaaacc ggagacgcau ggaaugccuu uguccaacga     1920

auagagacca auaaagagcu aagggguuuu aagaaaagga acuaucuauu aaagaaugca     1980

aaagaggcug aagaaaaauu cagaucccga aaccuaaacg acacgcgaua ugcagcaaga     2040

cuauucgcag aagcaguuaa auuauuauau gcguuugggg aaagacaaga aaaaggugga     2100

aauagacgag uauuuacaag gccaggagcg uugacagcag cauuaagaca agcauggggu     2160

guagagucuc ugaagaaaca agauggaaaa agaauaaacg augacagaca ucacgcauua     2220

gacgcccuaa cuguugcagc agucgacgaa gcagaaauac aaaggcuaac caaaucauuu     2280

cacgaauggg aacaacaagg auuaggaaga ccacuacgac guguagaacc cccaugggag     2340

ucuuucaggg cagauguaga agccacguac ccagaagucu uuguugcaag gcccgaaaga     2400

agacgagcaa gaggggaagg ucacgcagcu acaauacgac aaguaaaaga acgagaaugu     2460

acuccaauag uauuugaacg uaaagcuguu agcagccuga aagaagcuga uuuagaacgu     2520

auaaaagacg gagaaaggaa ugaagcaaua gucgaagcaa uccgaaguug gauugcaaca     2580

ggaagaccag cugaugcacc accgagaagu cccagaggug acauaauaac uaaaauaaga     2640

uuagccacaa cuauuaaagc agccguaccg guacgcgggg gaacagccgg aaggggugaa     2700

augguucgcg cagacguauu caguaaacca aaucgacgag gaaaagacga augguaccua     2760

guacccguau acccacauca aauuaugaau agaaaagccu ggccgaaacc cccuaugaga     2820

ucgauuguag caaacaaaga ugaagaugaa uggacagaag uaggucccga gcaccaguuc     2880

cgauuuaguc uuuacccaag aucaaacaua gaaauaauaa gaccaucagg agaagucaua     2940

gaggguuauu uuguuggguu gcauagaaau acuggugcgc uaacaauauc ugcacauaau     3000

gauccaaaaa gcauacauag uggaauaggu accaaaacgc uguuagcaau uagcaaauac     3060

caaguggauc gcuuuggaag aaagucgcca guccgcaaag aaguuagaac uuggcauggu     3120

gaagcaugua uaagucccac accgccaggu uga                                  3153


<210>  4953
<211>  3153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4953
augaaaauau uagguuuuga cauagguaua gcaagcauag guugggcauu uguggaaaac       60

ggagaacuaa aagauugugg aguaagaaua uuuacaaaag cagaaaaucc gaaaacaggc      120

gacucauuag caaugccuag aagagaagcg agaagcguaa gacgacgcuu agcacggcga      180

aaaggacgau uagaaacauu aaaaagacua cuagcaaaag aaugggacuu gugcuaugaa      240

gauuauauag cagcggacgg agaacuaccu aaagccuuua uggguaaaaa uuuaaacaac      300

ccauauguau uaagauauga ggcacuucaa agauuacuaa gcaaagaaga auuagugaga      360

guaguacucc auauagccaa acacagagga uacggaaaua aaaacgcuaa aauuacaaaa      420

agugaagaaa gcaaaagaga acaaggaaaa auccucucag cacucgccac uaaugcauca      480

gcaauagcaa gauacagaac cguaggggaa uauuuuuaua aagaguucug cgaaguuaua      540

aagaacccuc aagguuuauc aacaaaugaa cauuauacac aauccaaagu acggguauua      600

aagccaauac ggaacaaggg aggagaauau accaauugca uauuacaaga agacuuacaa      660

cgcgaacuuc gauguaucuu ugaauaccaa aaagguuuug gauucccaau aacacaggag      720

uuccaggaca agauacucaa aauagcauuc uaucaaagac cacuaaaaga cuuuucacau      780

uuaguaggaa aaugcacauu cuauccagac gaguucagag cuuuaaaaug cucccuauca      840

gccguagagu ucauaacauu aaguaaaaca aucaacguac ugacauacau cuccaaagaa      900

ucaggagaag uauacucaaa aggucaauac gaacaaaugu uagauucagu auucaaagca      960

guaugcgaaa aaggaucccu auccuucgcc caauuacgaa agauauuaca cauagaugaa     1020

agaauaaggu ucaaagaagu agauuauaug gcacgcaaaa gcaaaaauac aaguggggac     1080

aguacacaca aacagaaaac aucaaauccc gaaacaaaga aauuuauaga acacaagaau     1140

uuaaagaaau uuugugaaau auuaagcgaa aucgccgaag auagagcccu agcggauuca     1200

auagcaagag acaugacgcu uauuaaagac gaagaaaaac uugcccaaaa auuacacgga     1260

uacgguaaau ucucacaaga acaaauaacc caacuaagug ccuuaaauuu cagucaccac     1320

auaaaucuau cauuaaaagc auuaucagaa auuuuacccu uuaugcgcga aggaaugcga     1380

uaugacgaag cauguaaacu ugccggcuua aaagcacgac acaaugacaa gaaaucaaaa     1440

uuucuaccac cauuuuguga aagcauuuau gcagacgaau uaacaaaccc aguagugcac     1500

agagcuaucg cagaauacag gaaaauuuua aacgcacuaa uagcgaaaua cgggucagua     1560

cacaaaauac auauugaacu cacaagagac guuggcaaaa auuuccaaga aagagaaaaa     1620

uauaagaaag aaauagaauc aaacuauaaa gccagagugc aagcaaugca agaaugugaa     1680

aaacuaggac uaacccucuc ugaaggaaau auacucaaac uaagauuauu ccgagaacaa     1740

aacgaaauau gcgucuacuc agguaggaaa auaacucuag caaaucuaaa agaacaaggu     1800

gcuuuagaaa uagaccacau acuacccuau ucacguucaa gcgacgauuc uuauaugaac     1860

aaagugcuag ucuuuacaaa cgaaaaccaa aauaaaggua acaaaacucc auaugaagca     1920

uucggaggag auucccaaaa auggggagaa auagaaagcc uagcauuaag aaguggauau     1980

cccaaaaaga aagccaaaag aauacuagau aaaggauucg gcgaucgaga agcaggauuc     2040

aaaucacgua acauaguaga uagcggauau aucgccagac uaauagccaa uuauacaaaa     2100

gaauaucuga aauuuuuacc auuagauucc cacgaaaaua cagcacuuau agccggugaa     2160

aaagauucaa aaauacaugu cgaagcagua aaaggcaugc ucaccgcaac uaugagacac     2220

uuuugggguu uaggaaguaa aaacagauau gaacauacgc accaugcagu ggaugcaaua     2280

auaauagccu acauaaaugc cgccaugaua caaagauuuu cacaauucag acagaaccag     2340

gagagcuuaa aagcacgauu cuacgccaaa gaacuagcga aagaagaguu caaaacacaa     2400

agagcauucu uugaaccuuu ucgcggguuu agagaacaag uccuagcaaa aguaggucaa     2460

auauuuguau caaggccucc aagaaaacgc gcaagagggg ccuuacacga agaaacguuu     2520

uacucaauag acgacaagaa acuuagcgaa acguauggag gcaaagaagg uguucaacgu     2580

gcucugagcu uaggaaaaau aagacaaauu gguacaaaaa uagucgcaaa cggaccuaug     2640

guacggguag acauuuuuaa acauaaacaa ucaaggaggu uuuaugcagu accaauauau     2700

acaauggacu ucgcauuagg cauccucccc aacaaagccg uaguaagcgg gaaagacacc     2760

aaaagcaaag uaauaaaaga cuggcuagaa auggauaguu gcuaugaguu cugcuucagu     2820

uuauucaaag acgaccucau agaaguucaa aaaucagaua uggaaaaacc cgaacuagcu     2880

uacuucguaa gccuagaugc uuccgacgga agaaucaagg uucgacacca cuguaacgau     2940

auaacaaaaa uuaacgaaaa ucaaaagaaa cuguuuucug aagcgauaga aaaagaaguc     3000

guuggacggg gaagcauaca aaacuuaaaa guuuucaaga aauauaaagu aucaccacuc     3060

ggagaaauua aagaagcuaa auaugaaccu cgacaaccaa uagcacucaa aaccucacca     3120

aagaaacaca ggaaacaaaa uaacgcgcaa uga                                  3153


<210>  4954
<211>  3156
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4954
augaacaauu acauacuagg auuagacauu ggcauaaccu cuguuggaua cggaauaguc       60

gauagcgaca caagagaaau uaaagaugcu ggaguaagac uauucccaga agcuaacguu      120

gauaauaacg aaggccgucg auccaaacga ggagcacgga gacuuaaacg cagaagaauc      180

cacaggcuag acagaguaaa acacuuacua gcagaauaug accuguuaga ccuaacaaau      240

auuccaaaau caacaaaccc auaccaaacu aggguaaaag gauuaaacga aaaauuaagc      300

aaagacgaac ucguaaucgc auuauuacau auagcaaaaa gaaggggcau ucauaaugua      360

aacgucauga uggacgacaa ugacucagga aacgagcuca gcacaaaaga ccaacugaag      420

aaaaaugcaa aagcacuguc ugacaaauac guaugugaac uucaauuaga aagauucgaa      480

caagauuaca aaguaagagg agagaaaaac cgauuuaaaa cugaggacuu cguccgggaa      540

gcaagaaaac uucuagaaac acaaagcaaa uucuucgaaa uagaccaaac auuuauuaug      600

agguauauag aauuaauaga aacaagacga gaguacuucg aaggaccagg aaaaggcuca      660

cccuucgguu gggaagguaa caucaagaaa ugguuugaac aaaugauggg acacugcacu      720

uacuuucccg aagaacuccg aagcguaaaa uacagcuaca gcgcagaacu guucaacgca      780

uugaaugauc uaaacaaucu aguaauaacc agagacgagg acgcaaaauu aaacuauggu      840

gaaaaauuuc aaauaaucga aaacguguuu aaacaaaaga aaacacccaa ucuaaaacaa      900

auagcaaucg aaauuggagu ccacgaaaca gaaauaaaag gcuauagagu aaauaaaagc      960

gguaaaccag aguucacuca auucaaacug uaccacgauc ugaaaaauau auuuaaagau     1020

ccaaaauacc uaaacgauau acaauuaaug gacaauauag cagaaauaau aacuauauac     1080

caagacgccg aaucuauaau aaaagaacug aaccaacuac cagaauuauu aucagaacga     1140

gaaaaagaga aaauaucagc auuaucuggc uacucuggaa cacaccgacu aucccuaaaa     1200

uguauaaacc uacuauuaga cgaccucugg gaaucaucac ucaaccaaau ggaauuauuu     1260

accaaacuca aucuaaaacc aaagaaaaua gacuuaucuc aacaacauaa aauaccaucg     1320

aagcuaguag acgauuucau uuuaaguccu guugucaaac gagcauuuau acaaucgaua     1380

caggucguaa acgcaauaau agauaaauac ggacuaccag aagacauaau aaucgaacua     1440

gcuagagaaa acaacucaga cgacagacga aaauuucuua aucaacuaca aaaacaaaac     1500

gaagaaacua gaaaacaagu agaaaaagua uuacgcgaau auggaaauga caaugcaaaa     1560

cgaaucguac agaaaauaaa acuucacaau augcaagagg gcaaauguuu auacucacuu     1620

aaagauauac cccuagaaga cuuacuaaga aauccacacc auuaugaggu ugaccacaua     1680

auaccuagaa gcguagccuu cgauaauagc augcacaaua aagucuuagu ucgcgccgau     1740

gaaaacucaa agaaaggaaa ccgcacacca uaccaauacc ucaacucauc agaaucaagc     1800

cuauccuaua augaauuuaa acaacauauc cuaaaccuua gcaaaaccaa agaccggauu     1860

acaaagaaga aaagagaaua ccuccuagaa gaacgagaca uaaacaaauu cgauguacaa     1920

aaagaauuua uaaauagaaa ccucgucgac acaagguacg caacaagaga auuaacuagc     1980

uuacucaaag cauauuucuc agccaauaac uuagacguaa aaguaaaaac aauaaauggu     2040

ucauucacaa acuaccuacg aaaagugugg aaauuugaca aagauagaaa uaaaggauau     2100

aaacaucacg cagaggaugc acuaauaauc gcgaacgcag acuuucuuuu uaaacauaac     2160

aaaaagcuaa gaaacaucaa uaagguauua gacgcaccau ccaaagaagu cgacaagaaa     2220

agaguuacag uacaaagcga agacgaguau aaccaaauau ucgaagacac ccaaaaagca     2280

caagccauaa agaaauucga aauaagaaaa uucucacauc guguugacaa gaaaccaaau     2340

agacaacuaa uuaaugauac ccuauauuca acacgaaaca uagacggaau agaguacgua     2400

guugaaucca ucaaagacau auauucagua aauaacgaca aaguaaaaac caaauuuaag     2460

aaagacccuc auagauuacu aauguauaga aaugauccac aaaccuuuga aaaauuugag     2520

aaaguauuua aacaauacga aagcgagaaa aauccauucg caaaauauua cgaagaaaca     2580

ggagagaaaa uacgaaaauu caguaaaaca ggccaagggc cuuacauaaa caaaauaaaa     2640

uaccugcgag aacgguuagg cagacacugu gauguuacaa acaaauauau aaauagcaga     2700

aauaaaaucg uucaacuaaa aaucuacuca uaccgauucg auauauauca auauggaaac     2760

aauuacaaaa ugauaacaau cuccuacaua gacuuagaac agaaaaguaa cuacuacuau     2820

auaucuagag aaaaauacga acagaaaaag aaagacaaac aaauagacga cucauacaaa     2880

uucaucggau cauucuauaa aaacgauauc aucaauuaca acggagaaau guaccgagua     2940

auugguguua acgacucaga gaaaaacaaa auacaacuag acaugauuga cauaucuauc     3000

aaagacuaca uggaacucaa uaauauaaag aaaacuggag uaauauacaa aacaauagga     3060

aaaucaacaa cucacauuga aaaauacacu acugacauau uaggaaaucu auacaaagcu     3120

gcacccccga agaaacccca acuaauuuuc aaauga                               3156


<210>  4955
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4955
auggaaaaua aaaaucuaca uuauauacua gguuuagauu ugggaaucgc aaguguagga       60

ugggcuguag uagaaauaga cgaaaaagag aaccccuuaa gacuaaucga uguaggcgua      120

agaaccuucg aacgagcaga aacacaaaaa ggagagagcu uagcuuuaag cagacgaucc      180

gcucgaagcg cacgccgguu aacacaaagg agaguagcuc gguuaaagaa agcaaaacgc      240

uuacucaaaa gugaaaauau uuuguuaagc accgaugaaa ggcugccaca ucaagugugg      300

caauuacgag uagaaggacu cgacagaaaa cuagaaagac aggaaugggc agcggugcug      360

cuccaucuca uaaaacaccg uggcuaucua ucgcagagaa agaaugaauc aaaaagugaa      420

aauaaagaac uaggcgcgcu acucucuggu guagcaagca aucaugaauu auuacaacag      480

gcaaccuauc gaacaccggc agagcuagca guaaagaaau uugaaguaga agaaggacau      540

auuaggaacc aacaaggcgc auauacacau acauucucaa gauuagacuu acuagcagaa      600

auggaacuac uuuuuagccg ucaacaacac uucggaaauc cauuugcauc agaaaaauua      660

uuagaaaauu uaacggcauu auugaugugg caaaaaccag cgcuuagugg agaagauaua      720

uuaaaaaugc uagguaaaug uaccuuugaa gaugaauaca aagcagcuaa aaauaccuau      780

acggcagaaa gauuuguaug gaucaccaaa cuuaauaauc uaagaauaca agaaaaugga      840

uuagaaagag cacucaguga uaaugagaga cuaauguuga ucgagcagcc uuacgaaaaa      900

gcuaaauuaa cauaugccca aguacguuca auacuuaacc ugucagacga ugcuauauuu      960

aagggaguca gguauucugg ggaagauaag aaggcuauag agacaaaaac caccuuaaug     1020

gaaaugaaag cauaucacca aauaaggaaa guacucgaag gaaauaacuu aaaggcagaa     1080

uggguggcac uaaaagcaaa ucccacccua cucgaugaaa uagguacagc auuuaguuua     1140

uauaaaacag augaagauau aagugccuau cuagcaggca aauuaucuca accaguccua     1200

aacgcacuau uagaaaaucu uucauuugac aaauuuauac aauuaucguu aaaggcuuua     1260

uauaaacuuc uaccacugau gcaacagggc uugagauacg augaggcuug uagagaaaua     1320

uauggggacc acuaugguaa gaaaacugaa gaaacgcauc auuuccuacc acaaauacca     1380

gccgaugaaa uacgaaaccc aguaguauua cgaaccuuaa cccaagcgcg aaaaguuauc     1440

aauggagucg ucagauuaua uggaagccca gccagaauac auauugaaac uggccgcgaa     1500

guuggaaaaa gcuacaaaga cagacaugaa cucaagaaaa gacaagaaga aaauaggaaa     1560

caaagagaga aaacaauuuc agaaauaaaa acauuauuuc cuaauuucuc uggugaacca     1620

aaaggaaaag acaucuuaaa aaugagacua uauuaccaac aaaaugcaaa guguuuauau     1680

ucuggcaaac cacuagaauu acauagacua uucgaacaaa aauauguuga aguagaucac     1740

gcacuacccc uaucacggac augggaugau agcuuuaaua acaaagugcu aguauuagcu     1800

aaugaaaacc aaaacaaagg gaaucuaacu ccauuugaau ggcuagaugg uaaaaacaau     1860

ucugaaagau ggagaacauu caaagcccua guagaaacau cagcauuccc uuaugcaaag     1920

aaacaaagaa uacucaguca aaaauuggau gaaaaaggau uuauagaaag aaaucucaau     1980

gauacaagau augucgcacg auuccuaugu aauuuuauag cagauaauau gcacuuaacg     2040

ggagagggaa aacgaaaagu auuugcaucg aacggacaaa uaaccgcucu uuugcgccga     2100

cgauggggac ucgcgaaaag cagggaagac aaugaccgac aucaugcacu cgacgcagua     2160

uuaguagcgu guaguacugu agcaaugcaa cagaaaauua cacgauucgu gagauuuaaa     2220

gcaggagaug uauuuacagg agaacgcaua gauagagaaa ccggcgaaau aaucccacua     2280

cauuucccaa cacccuggca auucuuuaaa caggaaguag aaauaagaau auucagcgac     2340

aauccuaaau uggaacuaga gaaccgauua cccgaccgcc cccaagcaaa ucacgaguuc     2400

guacaaccgc uauucgucag uaggaugccu acaagaaaaa ugaccgguca aggccacaug     2460

gaaacuguua aaucagccaa aagauuaaac gaaggaauau cgaugauuaa aaugccauua     2520

acuaaacuca aacucaaaga ccuugaacua augguaaaua gagaaagaga aaaagauuua     2580

uaugacgcgu uaaaaaccag auuagaagca uucaacgaug acccggcaaa agcguucgca     2640

gaaccauuua ugaagaaagg aggugcaauu guuaaaucug uacgcguaga acaaguacag     2700

aaaaguggag uguuaguacg acaaggaaau ggagucgcag acaaugcuag uaugguaaga     2760

guagacguau uuaccaaaga cggaaaauac uuuuuagucc caauauauac guggcaagug     2820

gcaaaaggaa uacucccaaa caaagcaguu auacaaggca aagaugaaga agauugggaa     2880

gacauagacg augcaacauu ucaauucuca cuucacccga acgaccugau cuccguaaaa     2940

acaaagaaag augaauuucu aggauauuuc aauggccuca acagacacac aggaggaaua     3000

aauauaagaa cacaugaccu cgaaaaauca aaaggaaaac agggcauauu ugaagguaua     3060

gggguaaaaa uagcacucag cuuugaaaaa uaucaaauag augaacuagg gaaaaauaua     3120

agacuaugua aaccgucuaa gagacaacca guaagguga                            3159


<210>  4956
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4956
augaaaaaua cgagucuaaa cuauguccua ggcuuagauu uaggcauauc aucaguuggg       60

ugggcaguag uagaaauaga ugaaaaagaa aacccauuau cuuuaauaga cguuggugua      120

agaacuuuug aacgugcuga aacucaaaaa ggcgaaaguu uagcauuagc caggagauua      180

gcgagauccg caagacgccu aacacaacgc aggguugcaa gacucaagaa agccaaaaga      240

cuuuuaaaaa gugaaaacau acuccuaagu acagacgaau gccuaccuca ccaaguuugg      300

caauuaagag uagaaggacu ugaaagaaaa cuagaaagac aagaaugggc agcaguauua      360

cuacauuuaa uuaaacacag agguuaucuu agccaaagga aaaaugaauc uaaaucagaa      420

aauaaagaau uaggagcacu uuuaucaggc guagcaaaua accauaaacu gcuacaacag      480

acuacguaca gaacaccagc agaauuagcc gugaagaaau uugaaguaga agaaggacau      540

auaaggaauc aacaaggaag uuauacacau acauucaguc gacuggaccu auuaagcgaa      600

auggaauuau uauuuucuag acaacaacac uuugguaacc cuuucacauc agaaaaacuu      660

cuugaaaacu uaacggcauu auuaaugugg cagaaaccag cacucagugg agaugcaaua      720

uuaaaaaugu uagggaaaug uacauucgag aacgaauaca aagcagccaa acauacauau      780

agugcugaaa gauucauaug gaucacaaaa cucaacaauc uacgaauaca agaaaauggu      840

uuggagaggg cauuaaauga uaacgaaaga uuggcuuuaa ugaaacaacc auaugaaaaa      900

gcuaaauuaa cuuaugcaca aguaagaucg aucuuaaauu uaagugacga agcuauauuc      960

aagggacuaa gauacucagg agaagauaag aaaacauccg aaacaaaaac aguacuaaug     1020

gaaaugaaag cauaucauca aauacgacua gcacuagaga aaaaugauuu aaaaacagaa     1080

ugggcagaac uaaaaguaaa cagcuccuua cucgacgaaa uagguacagc auucagcuua     1140

uacaaaacgg augaagauau aucaaccugc cuugccggua aacuaucaca gccaguauug     1200

aaugcacucc uugaaaaucu caauuucgac aaauucaucc aacuaucucu uaaagcccua     1260

cacaaaauuu uaccuuuaau gcaacaaggu cuuagauaug acgaagcaug ugcagaaaua     1320

uauggagauc acuauggaaa gaaaacugaa gaaaaucauc uucuacuucc acagauacca     1380

gcugaugaaa uuaggaaucc uguuguuuua cgaacucuca cacaagcacg uaaaguaauc     1440

aauggugucg ugcgacuuua uagcagccca gcuagaauac auauagaaac agguagagaa     1500

guaggaaaaa guuaccaaga ccguagagaa uuagaaaaac gccaagaaga aaacagaaag     1560

cagagagaaa aagcuauaag cgagauaaaa acguuauuuc caaacuuuag uggugaaccg     1620

aaagcuaaag auauauuaaa gaugcguuug uacuaucaac aaaaugcaaa augccuguac     1680

ucagggaaac cauuagaauu acacagacug cucgaacaag gauauguaga aauagaccau     1740

gcacuaccgu uuucgagaac augggaugau ucauucaaca acaaaguauu aguacuggcu     1800

aaugaaaacc aaaauaaagg caaccuaaca cccuuugaau gguuggacgg caagaacaau     1860

ucugaaagau ggaaggcauu caaggcuuug guagaaacua gcgcuuuucc cuauacaaag     1920

aaacaaagaa uauuaaguca gaaacuugac gaaaaaggcu uuauagaaag gaauuugaau     1980

gauacaaggu auguagcgcg auuuuuaugc aacuuuaucg cggacaauau gcaccucaca     2040

ggagaaggaa aacguaaagu auuugccuca aacggacaaa uuacagcguu auuacgcggc     2100

agauggggcc uugcuaaauc aagagaagac aaugaccgcc accacgcauu agaugcggua     2160

guaguagcau gcucaaccgu aucaaugcag cagaaaauaa caagauuugu acguuuugaa     2220

gaaggaaaug uauucaccgg ugagaggauc gacagagaga caggggaaau aaucccacuu     2280

cauuucccag caccauggaa auucuuuaga gaagaaguug aaaucagaau uuuuagcgau     2340

aacccaaaac ucgaauuaga gaacagguug auagcaagac cgcaagccaa ucacgaauuu     2400

guucaaccgu uauuuguauc aaggaugccc acuaggaaaa ugacuggaca aggucauaug     2460

gagacaauua aaucagcaaa aagacuaaau gaaggauuau caguaauaaa aauguuacuc     2520

acaaaacuaa gacuaaagga uuuagaauca augguaaaua gagaaagaga aaaagaauua     2580

uacgaggcuc ugaaagcaag acuagaggcu uuuaacgaug aaccggcaaa agcauucgca     2640

gaaccauuca ugaagaaagg cggugccaua gugaaaagcg uaagaguuga acaaguccaa     2700

aaaucuggag uacuuguaag ggacggaaac ggaguagcag acaaugcgag uaugguaaga     2760

guagaugucu ucaccaaagg cgguaaauac uuccuugucc caauauauac guggcaagua     2820

gcuaaaggga uucuaccaaa uaaagcgguc auccaaggga aaaacgaaga agauugggaa     2880

gugauggacg augcaacauu ucguuuuagc cuacaccaaa augacuuagu aaaacuuguu     2940

acaaagaaga aaacuauauu uggauacuuu aacggacuga aucgagcuac aggcaacaua     3000

gauauaaaag aacaugaccu agagaaaagu aaaguaaaag acggaauaca ccaaaguaua     3060

ggaauaaaac uagcgcuuag cuuugaaaaa uaccagguag augaacuagg uaaaaacaua     3120

agauugugua aaucaucuaa aagacaagca guaagguga                            3159


<210>  4957
<211>  3159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4957
augauaggug aacaugucag aggaggaugu uuauucgaug accauuggac accaaacugg       60

ggagcauuuc gauuaccuaa uacaguacga acauucacga aagcagaaaa uccgaaagac      120

ggaagcagcu uagcagaacc uaggagacaa gcaagaggac ucaggagaag auuaagaaga      180

aaaacccaaa gacuagaaga uuuacgaaga uuguuggcua aagaaggagu acugucucuc      240

agcgaccuag aaacguuauu cagagaaacu ccagcaaaag auccuuacca acuucgggcc      300

gaaggauuag acagaccuuu gucauucccc gaauggguca gaguucuaua ucauaucacg      360

aaacauagag gauuccaaag uaauagaaga aaccccguag aagauggaca agagagaucu      420

agacaagaag aagaaggaaa auuauuaagu ggggucggug aaaaugaaag auuauuaaga      480

gaaggagguu auaggacagc aggagaaaug cuagcuagag acccaaaauu ucaagaucau      540

agaagaaauc gagcaggaga uuacagucau acuuuaucac gcagcuuauu auuagaagaa      600

gcaagacgau uguuucaaag ucagagaaca uuaggaaauc cacacgccuc cucaaaucuu      660

gaagaagccu uucugcacuu aguagcuuuu caaaauccuu uugcaucagg agaagacaua      720

agaaauaaag caggucauug cagccuagaa ccggaucaaa uaagagcacc acgcagaagu      780

gcaagcgcgg aaacauuuau guuacuacag aaaaccggaa aucuuagacu aauccauaga      840

cguacaggag aagaaagacc uuuaacagau aaagaacggg aacaaauaca cuuacuagcu      900

uggaaacaag agaaaguuac gcauaaaaca uugagacgcc aucucgagau acccgaagaa      960

ugguuguuua caggccuccc uuaccauaga aguggugaca aagcagaaga aaaacuguuc     1020

guacauuuag cugguauuca ugaaaucagg aaagcguuag acaaaggacc agaucccgcg     1080

guuugggaca cguugagguc ucgaagggau cucuuagauu ccauugcuga cacuuuaaca     1140

uuuuauaaaa acgaagacga aauacuaccg agauuagaaa gccucggacu aucaccagaa     1200

aacgcaagag cauuagcacc acuuagcuuc agugggacag cacaccugag ucuaagcgcc     1260

uuaggaaaau uauuaccaca uuuagaagaa ggcaaaucau acacacaagc ucgggcagau     1320

gcuggauaug cggcaccucc accagauaga cacccuaaau uaccuccauu agaagaagca     1380

gacuggagaa auccuguagu auucagggcu cuuacucaaa cuagaaaagu uguaaaugca     1440

uugguucgaa gauacggucc accguggugu auacaucugg aaacugcuag agaacugagu     1500

caacccgcaa aaguucgacg aagaauagaa acagaacaac aagcuaauga aaagaagaaa     1560

caacaagcag aaagggaauu uuuagauaua guagguaccg cuccuggucc aggugauuug     1620

cuuaaaaugc gacuauggcg ugaacaagga ggauucugcc cguacuguga ggaauaccua     1680

aaucccacaa gacucgcgga accaggauau gcagagaugg accauauucu accauauagc     1740

agaagucuug auaacgggug gcauaauaga guuuuagucc acggaaaaga uaaucgagau     1800

aaagguaauc guacgcccuu ugaggcauuc gggggugaca cagcaaggug ggacagauua     1860

guagcauggg uacaagcguc gcacuuauca gcaccaaaga aaaggaacuu guuacgagaa     1920

gauuuuggag aagaagcuga acgugaauua aaagauagaa auuuaacuga uacgcgauuc     1980

auaacgaaaa cugcagccac auugcucaga gacagauuaa cguuucaucc ggaagcgcca     2040

aaagacccag uaaugacacu uaauggaaga uuaacagcau uucuaagaaa acaauggggu     2100

cuacauaaaa aucgcaaaaa uggagaccuu caccaugcuu uagacgcagc aguacucgcc     2160

gucgcaucuc gaaguuuugu uuacagacua ucaucacaua augcagcaug gggugaacua     2220

ccaagaggaa gagaagccga gaauggauuc agucucccau aucccgcauu uagaucagaa     2280

guauuagcaa gauuaugucc uacaagagaa gaaauacuuu uaagauuaga ucaaggaggu     2340

guuggauacg augaagcguu cagaaauggu cuacggcccg ucuucgucuc uagagcacca     2400

agucgaagac uuagaggaaa agcacacaug gaaacacuac gcucaccaaa auggaaagac     2460

cauccagaag gcccaagaac agcaucaaga auaccacuua aagacuuaaa ccuagaaaaa     2520

cuagaaagaa ugguaggaaa agaucgagac agaaaacuau acgaggcauu gagggaacga     2580

uuagcagcau ucggaggcaa uggaaagaaa gccuuugugg cucccuuuag aaaacccugc     2640

agauccggag aaggaccacu cguacgaagu cuaaggauau uugauagugg auacagcgga     2700

guagaacuuc gagauggugg agaaguauac gcagucgcug accacgaaag uaugguaaga     2760

guugacgucu acgcgaagaa aaaccgcuuu uaucuagugc cuguauaugu agccgacgua     2820

gcgcggggua uaguuaaaaa uagagcaauc guagcacaca aaucagaaga ggaaugggac     2880

cuaguagaug gaaguuuuga uuuuagauuu agcuuauuuc ccggagaucu aguagaaauu     2940

gaaaagaaag auggagcgua ccucggauau uauaaaucau gucacagagg agauggucga     3000

cuuuuauugg aucgacacga ccguaugccu cgagaauccg auuguggcac auucuacgua     3060

ucaacuagaa aagauguacu aaguaugagu aaauaucaag uagauccacu aggagaaaua     3120

agguuaguug gaagugaaaa accuccuuuu guucuuuga                            3159


<210>  4958
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4958
augaaaagaa acuauauuuu gggacucgac auuggcauaa caaguguagg auacgguauu       60

auagacuacg aaacaagaga cguuauagau gccggaguaa gacuauucaa agaagcaaac      120

guagaaaaca acgaaggaag aaggucuaag agaggcgcaa gaagacuaaa aaggagaagg      180

cggcaccgua uccaacgagu gaaaaaauua uuguuugauu acaaucuacu cacagaccac      240

uccgaacuau cgggcaucaa uccauaugaa gcgcguguua aaggacuaag ccagaaacua      300

ucagaagaag aauucucagc agcccuacua cauuuagcaa aacguagagg cguacacaac      360

gugaacgaag uggaggaaga uaccggaaac gaauuaucca caaaagaaca aauaucucgu      420

aacuccaaag cguuagaaga aaaguacgua gcagaacuac aauuagaacg auuaaaaaaa      480

gacggagaag uacgcggauc uauaaaccga uucaaaacaa gcgauuaugu aaaggaagca      540

aaacaacugu uaaaaguaca aaaagcuuac caccaauuag accaaucauu cauagauaca      600

uacaucgacu uacuagaaac aagaagaacu uauuacgaag gaccaggaga aggaucccca      660

uucggcugga aagacauaaa agaaugguac gaaaugcuaa uggggcacug uaccuacuuc      720

ccggaagaac uaagaucagu aaaauacgca uacaacgccg aucuguacaa ugcacuaaau      780

gaucuaaaca aucuaguaau aacacgugac gaaaaugaaa aacuagaaua cuaugaaaaa      840

uuccagauaa ucgaaaaugu auuuaaacaa aaaaaaaaac caacacuaaa acaaauagcu      900

aaagaaauac uaguuaauga ggaagacaua aaaggauaca gagucaccuc cacaggcaaa      960

ccagaauuca cgaaccuaaa aguauaccac gacaucaaag acauaacagc aagaaaagaa     1020

auaauagaaa augcagaacu acucgaccaa auugcaaaaa uccuaacaau auaccaaagu     1080

agcgaagaca uacaagaaga acugaccaau uuaaacagug agcuaacaca ggaagaaauc     1140

gaacaaauau caaaucuuaa aggauacacu ggcacccaua aucugucucu caaggcaauc     1200

aaccuaaucc uagaugaacu uuggcauaca aaugacaauc aaauagcgau auucaaccga     1260

uuaaaacuag uaccaaaaaa aguggaccua agccaacaaa aggaaauacc cacaacacua     1320

guugacgacu uuauauuauc accuguagua aaaagaucuu ucauacaguc gauaaaagua     1380

auuaaugcca ucauaaagaa auacggauua cccaacgaua ucaucaucga acuugcgaga     1440

gaaaaaaacu cuaaagacgc acaaaaaaug aucaacgaaa ugcaaaaacg aaacagacaa     1500

accaacgaac guauagaaga aaucauacgc acaacaggua aagaaaacgc uaaauaccuc     1560

auagagaaaa uaaaacuuca ugacaugcaa gaaggcaaau gucuauacuc cuuagaagcc     1620

aucccucuag aagaucuacu uaauaacccc uucaacuacg aaguagacca cauaaucccu     1680

agaagcguaa guuuugacaa uuccuuuaau aauaaaguac ugguaaaaca agaagaaaau     1740

aguaaaaaag gcaacagaac uccauuucaa uaccucucca gcagugacag uaaaaucuca     1800

uaugaaacau ucaaaaagca cauucuaaac uuagcaaaag gaaaaggaag aauaucaaaa     1860

acaaaaaaag aauaccuccu agaagaaaga gacaucaauc gauucagugu ccagaaagau     1920

uucauaaaua gaaaccuagu agacacacga uacgcaacac gcggacuaau gaaccuccuc     1980

agaagcuacu ucagaguaaa uaauuuagac gucaaaguaa aaucaauaaa uggcggcuuc     2040

acuagcuucu uaagaagaaa auggaaauuu aaaaaagaac ggaacaaagg auacaaacac     2100

caugcugagg augcccuaau aauagccaac gccgacuuca ucuuuaagga auggaaaaaa     2160

uuagacaaag cuaaaaaagu uauggaaaac caaauguucg aagagaaaca ggcagaaagu     2220

augccagaaa uagagacaga acaagaauac aaagaaaucu uuauaacacc acaccaaaua     2280

aaacacauua aagacuucaa agacuacaaa uauucccaca ggguagacaa aaaaccaaac     2340

agagaacuca uaaaugauac auuauacagc accagaaaag acgacaaagg aaauacacua     2400

aucguaaaca accuaaaugg auuauacgau aaagauaacg auaaacuaaa aaaacucauu     2460

aacaaaucuc cagaaaagcu acuaauguac caucaugacc cacagacaua ucaaaaacua     2520

aaauuaauaa uggaacaaua cggcgacgaa aagaauccac uauacaaaua uuaugaagag     2580

acaggaaauu accuaacgaa auauagcaaa aaagacaaug gacccguaau aaaaaaaaua     2640

aaauauuacg gaaacaaacu aaaugcacac cuagacauaa cggacgauua uccaaauagc     2700

agaaacaaag uaguaaaacu aucacuaaaa ccuuacagau uugacguuua ccucgacaau     2760

ggagucuaca aauuuguaac agucaagaau cuagacguaa uaaaaaaaga aaacuacuau     2820

gaaguaaacu caaaaugcua ugaagaagca aaaaaauuaa aaaaaauaag caaccaagcc     2880

gaguuuauag caaguuucua caauaaugac cuaauaaaaa uaaacggaga auuauacaga     2940

guaauaggag ucaacaauga ucuacuuaau cgaauagaag ucaauaugau agacauaacu     3000

uacagagagu accuagaaaa uaugaacgac aaacgacccc cuagaauuau aaaaacuauu     3060

gcaagcaaaa cacaaagcau caaaaaguau ucaacagaca uacucggcaa ucucuacgag     3120

guaaagucaa agaaacaccc ccaaaucaua aaaaaaggcu ga                        3162


<210>  4959
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4959
augaaaagaa acuacauucu uggacucgau auaggaauca caaguguugg cuacgggaua       60

auagacuaug aaacucgcaa ugugauagac gccggaguac gguuauuuaa agaagcaaac      120

guagaaaaca augaaggaag acgaagcaaa agaggaacaa gaagacuaaa aaggaggaga      180

cgccacagac uccaaagagu aaaaaaauua uuauuugacc auaauuucuu gacagacaac      240

ucagaguuaa guggaauauc accauaugag gcacgaguca aaggucucaa ucaaaaaaua      300

ucggaagaag aauucucagu agcacuguug caucuugcca aacgaagagg gguacauaac      360

guuaacgaag uagaagaaga cacaggcaau gaacuuagua cuaaagaaca aauaucccga      420

aacucgaaag cccuagaaga aaaauaugua gcagaauuac aacuagaaag auuaaaagua      480

gauggagaag uacgcggagc gguuaaucga uucaagacaa gcgacuacgu gaaagaagcu      540

caacaaauau uaaaaguaca gaaagcauau caucaauucg accaaagcuu cauagacaca      600

uauaucgacc uacuagaaac acgaagaacg uacuacgaag gaccgggaga gggaucaccc      660

uucgguugga aagacauuaa agaaugguac gaaauguuaa ugggacaaug cacauacuuc      720

cccgaagagc ugagaagcgu uaaauacgcc uauaaugcag accuuuacaa cgcacuaaac      780

gaccugaaua aucuaguuau agcaagagac gaaaacgaaa agcuugaaua uuaugaaaaa      840

uuccagauca uagaaaacgu guucaagcaa aaaaaaaaac caacucuuaa acaaauagcc      900

aaagaaauac ucguaaauga ggaagauaua aaggguuaua gaguaacauc cacaggaaaa      960

ccggaauuca cuaauuucaa aguauaccau gacauaaaau caauaaccag uagaaaagag     1020

auacucgaga acgccgaauu gcuaaaccaa auagcaaaaa uacuuaccau uuaccaauca     1080

uccgaggaca uacaaggaga acuagcacga cuauacucgg aauuaaccca agaagaaauc     1140

gaacaaauaa gcaaacuagc cggauacaca ggcacccaca gacuaucauu aaaagcaaua     1200

aaccugauca uagacgaacu auggcacaca agcgacaauc aaauggaaau auuuaauaga     1260

uuaaagcuag ucccaaaaaa aguugacuua ucacaacaaa aagaaauacc aaccacucua     1320

gucgaugacu ucauauuauc uccuguugua aaaaggaguu ucauacaaag cauuaaaguc     1380

auaaacgcaa uaauaaaaaa auaugguuuc cccaaugaca uaaucaucga acuagcaaga     1440

gaaaaaaaua guaaagacgc acaaaaauuc aucaaugaaa ugcaaaagag gaacagacaa     1500

acaaaugaga gaauagaaga gauaauaaga acuacaggaa aagaaaaugc caaauaccuc     1560

auagagaaaa uaaaauuaca cgacaugcaa gagggcaaau guuuauacuc ucucgaagca     1620

auaccacuag aagaucuacu aaacaauccc uuuaauuaug agguagauca cauaauuccg     1680

agaucuguau ccuuugauaa uucauuuaac aauaaaguuc uaguaaaaca agaagaaaac     1740

ucaaaaaaag gaaauagaac accguuccaa uacuucucau ccucagauuc aaaaauaagu     1800

uacgaaauau ucaaaaaaca cauccucaau uuagcaaaag gaaaagggcg cauaucaaaa     1860

aaaaaaaaag aguacuuacu agaagaaagg gacauaaaua gauucucagu acaaaaauac     1920

uucauaaaca gaaaucuugu agauacccgc uacgcaacaa gagaacucau gaaccuacuu     1980

aggagcuauu uccgaguaaa ugaucuugau auuaaaguaa aaaguauaaa uggaggcuuc     2040

acaagcuucu uacguaagaa auggaaauuc aaaaaagaac gcaacaaagg auacaaacac     2100

caugcugaag acgcacuaau aauagcuaac gcagacuuua ucuuuaaaga auggaaaaaa     2160

cuagacaaaa caaacaaagu uauggaagau caaauagucg aagaacaaca agcagaguca     2220

augcccgaga uagaaacaga acaagaauau aaagaaauuu ucauaacucc acaccagauu     2280

caagauauua aggacuucaa agauuauaaa uauucacaca gaguagacaa gaaaccaaac     2340

agagaacuug uaaacgauac ccuguacuca acaagaaaag acgacaaggg gaacacgcuu     2400

auaguuaaua acuuaaaagg ucucuaugac aaagacaacg acaaacugaa aaaacugaua     2460

aacaaaucac ccgaaaaacu acuuauguau caucaugacc cacaaacaua ucaaaaacuc     2520

aaaguaauca uggaacagua uucuaaugaa aaaaacccgu uauacaaaua ccaugaagaa     2580

accggaaacu aucuuacaaa auauucaaaa aaagauaacg gaccaguaau aaaaaaaauu     2640

aaauauuacg gcaacaaauu aaauguguac cuagacauaa cacgggauua cuuaaaauca     2700

aggaacaaaa uaguaaaacu aagcuuaaaa ccauaccgcu ucgaugucua cuuagacaau     2760

ggagucuaca aauuuguuac aguaaaaaau uuagacauaa uuaagaaaga agauuauuau     2820

gaaguagacu ccgauucaua cgaagaagca aagauaguca agaaaguuuc ugaacaagca     2880

caauucguag caagcuucua uaacaacgac cuuauaaaag uugauggcga acuauucaga     2940

guaauuggcg uaaacaauga cuuacuaaac agaauagaag uaaauauggu agacauuaca     3000

uauaaggaau aucuagagaa caucaaugac aaaaaaucaa gccgaaucau aaaaacaauc     3060

aguucuaaaa cacaaaguau aaaaaaauac agcacugaca uuuuaggaaa uuuauacgaa     3120

gcaaaaucca aacaaagacc acaaaugaca augaaagguu ga                        3162


<210>  4960
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4960
augaaaagaa acuauauacu cggucuagau auaggaauaa caucaguugg uuacggaauc       60

auugacuacg aaacaagaga cguaauugac gccgguguaa ggcuauuuaa agaagcaaau      120

guagaaaaua augaaggaag aagaaguaaa agaggugccg gccgucuuaa aagaagacgu      180

cgacaccguc uacagcgcgu aaaaaaacuu cuauucgauu acaaccuauu aacugaccau      240

ucagaacucu caggcaucaa cccauacgaa gcaagaguca aaggccuauc acaaaaacua      300

uccgaagaag aauuuagcgc agcccuauua caccucgcaa aaagaagagg uguacauaau      360

guaaacgaag uagaggaaga cacggguaau gaacuaagua caaaagaaca aaucucccga      420

aauagcaaag cacuugaaga aaaguauguc gcagaauuac aauuagaaag gcuaaaaacc      480

gauggugaag uacguggaag cauaaauaga uucaaaacau ccgacuaugu caaagaagca      540

aaacaauuac uaaaaguaca aaaagaauau caccaauuag accaaucauu cauagacaca      600

uacauagauc uauuagaaac uagacgaaca uacuacgaag guccaggcga aggaucacca      660

uuugggugga aggacauaaa agaaugguac gaaaugcuca ugggacacug caccuauuuc      720

ccggaagaac ucagauccgu aaaauaugcc uacaacgcag accuuuauaa ugcacuaaac      780

gaccuuaaca acuuaguaau agcaagagau gaaaacgaga aauuagaaua uuacgaaaaa      840

uuccaaauaa uagaaaacgu cuuuaaacaa aaaaaaaaac cuacguuaaa acaaauagaa      900

aaagagauau uaguaaacga agaagauauc aaaggauaca gaguaacguc aacaggaaaa      960

cccgaauuca guaaccucaa aguauaccac gauauaaaag acauaacagc aagaaaagaa     1020

auaauugaga acgcagaacu gcuagaccaa auagccgaaa uacuaacgau auaucaauca     1080

ucugaggaua uccaagaaga acucgucaaa uuaaauucag aacuaacaca agaagaaaua     1140

gaacagauaa guaacuuaaa agguuauacg ggaacucaua accuaucauu gaaagcuaua     1200

aaucuaauau uagaugaauu auggcauaca aacgacaacc aaauggcaau auucaacaga     1260

cuaaaacuug uaccaaaaaa aguagaccua agccaacaaa aagaaauccc aacuacucuc     1320

guagaugacu ucauacuauc cccaguaguc aaaagaagcu ucauacaaag cauaaaagua     1380

auaaacgcca uuauaaagaa auacggccuu ccaaaugaca ucaucaucga acuagcaaga     1440

gaaaaaaauu cuaaagaugc ccaaaaaaug aucaaugaaa ugcaaaaacg uaaucgacag     1500

accaaugaga gaauagaaga aauaauaagg accacuggaa aagaaaacgc aaaauaccug     1560

auagaaaaaa ucaaacuaca cgacaugcaa gaaggcaaau gcuuauauuc ucuagaagcc     1620

auaccgcuag aggacuuauu aaacaaucca uuuaacuacg aaguugauca uaucauaccg     1680

aggucaguua guuucgacaa cucguucaac aauaaagucc uagucaaaca agaagagaac     1740

aguaaaaaag gaaaccgaac accuuuucag uaucucagcu ccucagauuc aaaaauauca     1800

uaugaaaccu uuaaaaaaca cauacuaaau uuagcaaaag gaaaaggacg aauaucaaaa     1860

accaaaaaag aauaucuucu ugaagaaaga gauaucaaca gauuuagugu acagaaagau     1920

uucauuaauc gaaaccuagu ggacacgcga uaugcgacca gagaacucau gaaccuauua     1980

agaaguuacu uuagaguaaa caacuuagau guaaaaguaa aaucaauaaa cggcggauuu     2040

acaucauucc uucgaagaaa auggaaauuc aaaaaagagc gaaauaaagg auacaaacac     2100

cacgcagaag acgcacuaau aauagcaaau gcagacuuca uauuuaaaga auggaaaaaa     2160

uuagacaaag caaagaaagu uauggaaaau cagaccguag aagaaaaaca agcagaaagu     2220

augagcgaaa uagaaacaga acaagaauau aaagagaucu ucauaacacc ucaccaaauc     2280

caacacauua aagauuucaa agauuacaaa uacucacaua gaguagacaa aaaaccaaau     2340

agagaacuca uaaaugacac acucuacuca acaaggaaag acgacaaagg aaauacacuu     2400

aucguaaaca acuugaaugg uuuguaucac aaagacaacg acaaacuaaa aaaacuaaug     2460

aauaaaucac cagagaaacu acuaauguac caucacgacc cacagacaua ccaaaaacua     2520

aaauuaauaa uggaacaaua cggagaugaa aaaaauccac ucuacaaaua uuacgaggaa     2580

acaggaaacu auuuaacaaa auauucaaaa aaagacaacg gaccaguaau caaaaagauc     2640

aaauauuacg gaaacaaacu aaacgcgcac cuagacauaa cagacgacua cccuaacuca     2700

cguaauaaag ucguaaaacu uucacuaaaa ccauauagau ucgauguaua ucucgauaac     2760

ggagucuaca aguuuguuac cguuaaaaac cuaaacguca ucaaaaaaga aaauuauuau     2820

gaagugaaca guaaauguua cgaaaaagcc aaaaaacuga aaaaaauauc ggaccaagcc     2880

gaauuuauag cauccuuuua uaacaacgau uuaauaaaga uagauggcga acuauaucga     2940

gucauaggag uaaauacaga cuuaaucaac cgcaucgaag ucaacauggu agacaucaca     3000

uacagagaau accucgaaaa uaugaacgac aaaagaucgc ccagaaucuu uaaaacaaua     3060

gcaucaaaaa cacaauccau uaaaaaauau ucuacagaca uucuaggaac uuuauaugaa     3120

guaaacucaa aaaaacaccc ucaaaugaua augaaaggau ga                        3162


<210>  4961
<211>  3162
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4961
augaguggaa ugguuuucgg uauagauuua ggaauagcaa guuguggcug ggcugugcua       60

agacaaccac aaagagaugg cgaccccggc gagauaguag acuuaggaag uuggauguuu      120

gauguaccag agacugauaa agaaagaaca ccuacaaacc aaguucgcag aggcaaccgu      180

uuguuaagaa ggguaauaag aagaagggcg caaaggaugg uggagauuag aagacuuuuc      240

caugaccaug gacuauuagc uggacacgca ccugaagcac ugaaaagagc agguuuggau      300

ccuugggacc uuagagccag gucgcuagac aaagucuugg agccagcugc ccuagcggua      360

gcuuuaggac auaucgcgaa aaggagggga uucaaauccg cagcaagaag gaaagaagcu      420

aauacagccg gagaugauca gaaaauguua aaggcuuugg aggccacaca ugaaagacuu      480

ggaagauaua gaacaauugg agagauguuc gccagagacc cagauuuuga gucaagaaaa      540

agaaaucgag auggaauguu ugacagaaca caaggucgag augauuuauu acaugaagua      600

ggcgaaaucu uuaaagcaca aaggaggcuc ggcagugcau uagcaacugc ugaauuggaa      660

caggcuuuca cagcaauagc cuuuagacaa uuaccaauac aagauucaga acggcuagua      720

ggguuguguc aauuugagcc aaaagaaaag cgagcagcac gauucgcacc aaguuuugaa      780

agauuuaggu uguuacaacg auugacaaau uugagaguag uaacaguuga aggagaaaga      840

cccuuaacug cugaugaaau agcagcggca gcagcagauc uaggucgaac ugcaaaauua      900

uccguaaaag aaguacguaa acgaauugga uuagcagcag aucacagauu cgcggcuuuu      960

aaagcagaug aagaagauag agacauuaua gcaagaacag gagaggcacu acacgggaca     1020

uaucgauuga gaaaggcauu aggggaaggu uuaugggcgg aaauguuacc aggacaacuu     1080

gaugcaauug cacaugcacu uagcuucuuu gagacacaag augugauuuu aaaagaauua     1140

gacaaacuag auuugcccgc cggcgucaga gaugcaauug ccacaggcuu agaugcggga     1200

gcauuugcaa gauuuaaagg gacaggccac auaucagcac gugcggccag agcuuuacug     1260

ccgcaucugg aagcaggacu gagauaugau caagcgugca caaaagcagg uuaugaccau     1320

gccgcuucaa gaugggcaaa acgugaacag guagccgaca aagcagcauu uaacagauua     1380

guaacggaca ugggugcuga aauagcgaau ccuauagcaa gaaagagcuu aacggaagca     1440

uugaaacagc ugugggcgau gagaaauaga uggggacuuc cggaugcuau acauauagaa     1500

uuggcaaggg auguugguaa cucguuggag aaaagaagag aaaucgaaag agcaauugag     1560

aaaaacacug cagcgagaga aagagaaaga ggagaagcga gagaccuacu uggcauugau     1620

gaugucagug gcgauacucu ccuaagauau agacuaugga aggaacaggc aggcagaugu     1680

ccauauacgg augcaccaau cccaccagga gcaauaauag caaccgauaa caguuuucaa     1740

guggaucaca uauuacccug gaguagauuu ggugaugaua guuuugcaaa uaaaguacua     1800

ugugcaacag cagccaauca aagaaagaaa ggacaaacac cuugugaaug gauaacugca     1860

gcucaagggg aagaagggug ggccacauuu guagcuagaa uagaaggcaa ugcugcauuc     1920

aggggaccua agaaaaggaa cuauguauua aagaacgcga aagaagccga agaaagauuu     1980

agggcgagaa auuuaaauga uacaagauac gcagcaagac uauuggcuga ggcaguaaaa     2040

uuauucuauc cagaagguga aaggcaggac aaagguggag uaaggcgagu uuucaccaga     2100

ccaggaggac uaacagcggc ccuuagacau gcauggggcg uugaggcuuu aaagaaaaga     2160

gacgguaagc guguagauga cgcucgacac cacgcguuag acgcuuuagu agucgcagcu     2220

auaggagaag gugaaguuca aagacuaaca agaucauauc aagaauggga acaacaagga     2280

uuagcuaggc cauuaagacg aguagaucca ccauggggag auuuccauag cuucagacgc     2340

gaaguuaaag augccuacga uggaauuuuu guugcaagac cagaaagaag acgagcgaga     2400

ggugaaggcc acgcagccac gauaaggcaa guacgagaac gagauggggc agccgucguu     2460

uuugaacgga aagcaaucgc agaccugagu gagaaaagac ucgcagauau aaaggaccca     2520

gaaagaaauc aagcgauugu ugaagcuaua cgacaaugga uaguagacgg aagaccagca     2580

gauagauuac cgagaagucc agccggugau gaaauaagga aaguuagauu acgaacuaaa     2640

ggaaaaccgg caguacaagu acgaggaggg gcagcagauc gaggagaaau aguaagaguu     2700

gacguauuua ccaaaccaaa caagaaaggg aaaaaugaau uuuaccuggu accaauauac     2760

ccacaucaag ucaugaauaa agcagagugg ccaacgcccc cuaugagggc agucgucgcg     2820

uauaaagaug aaucugaaug gacccuacua gaugaaaacu ucggauuucu auucucauua     2880

uuuccaagau cauaugucga agugacuaaa ccgggaggag aaguauugag cggguacuuu     2940

caagguaugg auagaaguac aggagcgaua ucacuguuca aucaccgaga uucaagauca     3000

cuuacggaug auagcggaaa uaguacaaga ggaauaggug ccaaaacucu auuaacaaug     3060

aagaaauaua gcguggauag auuuggaaaa agagcugaag uuaaaucaga aguccggaca     3120

uggcacggug uagcguguac auauccuaca ccaccagggu ga                        3162


<210>  4962
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4962
augaaacuaa caccacucaa uuauauacua ggcuuggauc uaggaauagc aucaguaggg       60

ugggcggucg uagaaauaga cgaacaagaa aauccgcuug gacuuauuga uguagguguu      120

aggacuuuug auagagcaga agucgcaaaa acaggugaau ccuuagcauu agcucgaagg      180

uuggcgcgaa guucaagaag acuugugaaa aggagagcug acagaauaaa gaaaguaaag      240

agacuauuaa aaucagaaaa uauauuauua agcgcagaug aacaauuacc aaaugaugua      300

uggcaauuaa ggguaaaagg gcuagaucaa aaacuugaaa gacaagaaug ggcagccgua      360

cuacuacauu uacuaaaaca ucgaggguau uuaagccaaa ggaaaaacga aaguaaaagu      420

gaaaacaaag agcucggcgc acuaagaucc ggaguggcag cuaaucacca aauuuugcaa      480

ucagccgaau auagaacacc agcagagaua gcaguaaaga aauuccacgu agaagaagga      540

cauauaagaa accaaagagg agcauauacu cacaccuuua guaggcucga ucuacuagca      600

gaaauagaau uauuauucac aagacaaaca gaacucggca auccacauac aagcgcgaaa      660

uuguuagaaa accucaccgc cuugcuaaug uggcagaaac cugcauuagc aggagaggca      720

aucuuaaaaa uguuaggaaa auguacguuu gaaccuacgg aauacaaagc agcuaaaaac      780

uccuacucag cagagagguu cgucugguua acaaaacuaa auaacuuaag aauacucgaa      840

caaggagcag aaagagcacu aacagauaau gaaagguuug cucuacuaga ccaaccauac      900

gaaaaagcaa aauucacaua ugcacaagca agaacuaugc ucgcgcuacc agaugaggca      960

auauuuaaag ggguaaggua ccaaggagaa gauaagaaag cugucgaaac aaaaacaaua     1020

cuuauggaaa ugaaagcaua ucaucaaaua cgaaaagcac uggagaacgc cgaucuaaaa     1080

gcugaaugga acgaacugaa gaauaacuca gaacuacuug acgacauagg aacugcauuu     1140

aguuuacaua aaacagauga ggacauaugc cgauaccuug acgguaaauu aucagaaagc     1200

auauugaacg cacuacuaga aaaucuaaau uuugacaaau uuauucaguu aagccucaag     1260

gcuuuacaac aaauucuacc ccuaaugcua caaggacaaa gauaugauga agcuguaucc     1320

gcaaucuaug gagaccauua cggaaagaaa agcgcugaaa uuaaccgcuu auuaccuaca     1380

aucccugcgg augaaauaag aaacccagua guacucagaa cucucacgca ggcaagaaaa     1440

guaauaaaug cagugguaag auuauacgga uccccugcac gaauacacau ugaaaccgga     1500

agagaaguag gaaaaucaua ucaagauaga aagaaacugg aaaaacaaca agaagauaau     1560

agaaaacaua gagaaagugc uguacaaaaa uuuaaagaau auuuuccaaa uuuuguuggu     1620

gaaccuaaag gcaaagauau ucuaaaaaug agauuauacg aauugcaaca agccaaaugu     1680

cuuuauuccg gaaaaucgcu agaauuacac cgcuuauugg aaaaaggaua ugucgagguc     1740

gaucacgcuc uaccguuuuc gagaacaugg gacgacucau uuaauaauaa aguauuaguc     1800

cuugcaaaug aaaaccaaaa uaaaggaaac uugacaccau augaauggcu agacgguaaa     1860

aauaauucgg aaccuuggca aaauuucguc gcaagagugc aaacaagcgg guucucacau     1920

acaaagaaac aaagaauauu gucacauaaa cuagaugaga aagguuuuau ugagagaaac     1980

uuaaaugaca cuagauaugu aguaagauuc uuaugcaauu uuauagcaga uaauaugcug     2040

uuaacaggua aaggaaaacg aaaaguauuu gcaucuaacg gacaaauuac agcuuuauua     2100

agaggucgau ggggacuaca aaaaguaaga gaugauaacg auagacauca ugcauuggau     2160

gcagugguag uagccuguuc aaccguagca augcaacaga aaauaaccag guuuguuaga     2220

uacgaagaag gaaacguauu cagcggagaa cgaauugaca gggagacagg ggaaauuaua     2280

ccacuucauu uucccagucc augggcguuc uucagggaga auguagaaau ccgaauauuu     2340

ucagaaaacc caaaauuaga acuagaaaau agauuaccag acuauccaca auauaaccau     2400

gaguucguac aaccacuauu uguaagucgu augccaacac gaaaaaugac gggacaagga     2460

cauauggaaa cuguuaaauc agcaaaaaga uuagaugaag gguuauccgu auuaaaagua     2520

ccauuaacac aauuaaaacu aaaugaccua gaaagaaugg ucaauagaga acgagaaaug     2580

gcauuauaug agucauuaaa aguacgucua gaacaauucg auaaugaucc agccaaagca     2640

uucgcugaac ccuuuuauaa gaaaggagga gcccuaguaa aagcaguaag agucgaacaa     2700

acacagaaaa gcgguguacu gguuagagac ggcaauggag uagcagacaa ugcgaguaug     2760

guacgugucg acguuuucac aaaagcaggu aaauauuuuc uugucccuau uuauacaugg     2820

caagucgcaa aagguauccu accuaauaaa gcaguaacgg caaacguaga ugaaauagau     2880

uggcuagaga uggacgagag cuaucaauuc auauucacua uguauccgaa ugaucugguc     2940

aaagucaaau uaaagaaaga agaguucuuu ggauauuaug gaggacuuga uagagcaacu     3000

ggagcuauag ugauuaaaga acaugaucua gaaaaaucaa aagguagaca aggaaucuau     3060

agaauuggug uaaaauuagc acuaaguuuc gaaaaauauc aaguagauga auuaggcaaa     3120

aacauaagac ccugucgucc aacaaaaaga caacacguua gauga                     3165


<210>  4963
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4963
augcgaauac ucggaguaga ccuaggagua gccucaguug guugggcuuu agugaacgau       60

aaaaauaaua aaauaauaga uucaggaguu cgcauauuca cacagucaac caacagagag      120

ggaaaaacac uagccgcagu acgacgauca uuuaggggag gaagaagagu ccuaaguaga      180

agaaaacaaa gauuaaacca auuaaaaaaa cuauuuauug cccacaaauu gcuaucaaaa      240

ucagaauuca aacaccucuu caacagcaaa aacagacuag acauauggca acuacgugca      300

aaaggacuug aacaacaacu aaacaacaaa gaaugggcga gaguacugua ucacauugca      360

aaacgccgag cuuaccaauc aaaucguaaa uccgaagaau cuggaaacac cgacaaaaaa      420

aaaguacuaa guggcaucuc aaaaaacgag aaaaaaauga acgaauuagg uuacaaaaca      480

auuggcgaaa uguuauauac ugaaaauaga uacccugaua aucggauaag aagaaauaaa      540

aaagaccagu auaaacacuc aaucagcaga gauuuacuaa gaaaagaaau agacguucua      600

uuugaaaguc aaagagauuu ugguaaucag uuugcuacug auggauuaaa agaacacuac      660

aaagauaucg cauucacaca aagaccaaua aaauucaaag cagauuuagu cgguaaaugu      720

accuucgaaa cacaagagua ccgaggugcu aaaaacugcu ucagugucga aaaaucaacu      780

cuccuaucaa aaaucaauaa cacagaccua auagacaaau caaccggcga aauuaaaaga      840

uuauuugaaa uaagcgaauu cgauaaacua uuagauauau uuuauucaau caaagaagua      900

aaauacacaa cacuccgaaa acaacucaaa auuacggaug ccuuuaaauu caagcaauua      960

gacuaccauc aaaacuuuga auacaaaaca aacauaaaag ccaaaauaga agacuucuau     1020

aaauuagaag auuggcuaac uccagaacaa aaaguguuau uagaaaaaca aguucucgac     1080

gacaaaaaag aaaaauuuaa auguuacaaa uauagcucua uuagaucaau acucucacuu     1140

agugaauccc aaaaauucaa uggagugaaa uaugauacaa aaguggaaaa uacaguauuc     1200

ggaaaacuaa auggauacca uucaauuaaa aaagaacuaa acaaagaagu auucgaccua     1260

uuauucaucg auaaagacaa auucaacaaa auugcagaaa uacucucgua ccagaaagau     1320

gacaaaucag caacccaaga ucuaaaaaaa caaguauuca augauuuuaa cauggauaag     1380

acaauuguaa acgaagcaau caauucauua cuaaauauau cuuucucagg auucaacuca     1440

uuaucauuaa aagcaauaaa caaauuguua ccauauuuau gccaaggacu aaaauaccau     1500

gaugcaguag cacacucgga auauaaacau cauagucaau ucaaggcagc aaauccucaa     1560

caauaccuac gaccucuaaa caaagaagaa aacuaccaga uuaccagucc aacuguaaaa     1620

cgagucuucu cucaauuccg aaaaguucua aaugcaguaa uacgcaaaca uggcagcuuc     1680

gaugcaaugc acauugaaau ggccagagaa cucaaaaauu caaaaaagau aaaauucgaa     1740

auacaaacag gacaaaagga auacgcacaa gaaaaagaag caauaagggu caaauuccua     1800

gaaauauuca aauacgaagg uuccggacga gaucuccuaa aacuuagauu auacgaacaa     1860

cagcauggca aauguaucua caguaaaaaa gaaauagaaa uaaauaaauu auuagaagac     1920

ggauauguag aaauugacca cauccuaccu uggucaagaa cauucgacaa uucacuaaac     1980

aacaaagcgc ugugcuuaag cgcugaaaac caaaacaaag ccaaucaaac accauucgaa     2040

uauuuaacga acuccgauaa aaauagcaaa gauaguaaag aauggcagga guacaagaac     2100

uauguaagcu cauucgcagg aauuaaacag gcaaaaaaac auaaacuacu aaauacaacg     2160

cuaccacccc gaagggguaa ugacuuuagc gaugauauaa aaaauccaga aucaggauuc     2220

uuagcaagaa aucucaacga cacagcauau gcuucacgau uuauaaaaaa cuucguacaa     2280

aaccaucuac aguucuccca aaaugacaca auaaaacaaa aagucaaagu acguagcggu     2340

gcacuaacaa accagcugag auacaauugg ggaauagaag aaaaaaaccg cgauaacaac     2400

cuacaucacg cggaagacgc aauuauauua gcauucgcaa cucaaggaga agugcaaagg     2460

augucaacaa uaagcgcaaa acgagaagac uucaaguauc aaacggccaa ggaacgaaaa     2520

auaaaauuca caccaccauu uuccgauuuc aaauccguau uagacgaauc aguaggcgac     2580

auuuucguua guuuugcacc cagacgaaaa auaucaggag cagcacacaa agcgacaaua     2640

aaaccuaaag auacaggagg uaaauacaaa uucccgguaa auaacggcau ggcagaaaau     2700

ggaguaauac agagagucga uguauuuaaa aaugcaaaaa auaaauauca auucauaguu     2760

uucuacccug cagauuuuua caaagacgac uucccagaau uagaccucaa gggcaacgaa     2820

auagauaaga acguccaauu uuuauuuuca cuauucaaag acgaccucau agaauuuaag     2880

accaaaaaaa caaaaacaaa agacaguaaa caucuuuuag cauacuucaa auacaucaga     2940

ucagauggaa aaauagccuu ccaacagaau uacaaagcug aaauagaaag aaaagaauca     3000

gacaauaaaa aaggauacaa agacauccua cuagcaacag ggaguggacu agccucauua     3060

aaaaaauauc aaguaagugu acuaggcgac auagcagaag uaaagaacga aaaaagauua     3120

ccacuaauaa aacaaaugua uaaaagcaaa ccgaagaaaa aauga                     3165


<210>  4964
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4964
augaaccaaa aauuuaucuu aggauuagac auaggcauaa caucaguugg auacggauua       60

auagacuaug aaaccaaaaa caucauagac gcaggaguaa gacuuuuccc ugaagcaaau      120

guagaaaaca augaaggacg gcgaaguaaa agaggaucaa gaagacucaa acgaagaaga      180

auacaucgac uagaacgugu caaaaaacua uuggaagacu auaauuuauu ggaucaauca      240

caaauaccac agaguaccaa uccauaugca auacgaguca agggccucuc agaagcauua      300

ucaaaagacg aacucguaau agccuuacuu cacauagcaa aacguagagg aauccauaag      360

auagacguaa uugacucaaa cgacgacgua ggcaacgagc uaucuacaaa agaacaacua      420

aauaaaaacu cuaaacuacu caaagacaag uucgucugcc aaauccaacu agaacgaaug      480

aacgaagggc aaguacgcgg cgaaaagaac agauucaaaa ccgcagauau aauaaaagag      540

auaauacaac uauuaaacgu acaaaaaaac uuccaccagc uagacgaaaa uuucauuaau      600

aaauauauug aacuaguaga aaugcgcaga gaauauuuug aggguccagg gaaagguagc      660

ccauacggau gggaagggga ucccaaagcc ugguacgaga ccuugauggg acauugcacu      720

uacuuccccg augaacuucg aagugucaaa uacgccuauu cugccgaucu cuucaacgca      780

uuaaacgacc uuaauaacuu aguuauucaa agagacggac uaucaaaacu agaauaucau      840

gaaaaauauc auaucauaga aaaugucuuu aaacaaaaaa aaaaacccac acuaaaacaa      900

auagcaaacg aaauaaacgu aaauccagaa gacaucaaag gauacagaau aacuaaauca      960

ggaaaacccc aauucacaga auuuaaauua uaccacgauc ucaaaucugu acuauucgac     1020

caaucaaucc uagaaaacga agacguacuc gaucaaauag cagaaauacu gacaauauac     1080

caagacaaag acucaauuaa auccaaacua acagaauuag auauacuacu caaugaagaa     1140

gauaaagaaa acauagcgca acuaacugga uauaccggca cucacagacu gucacucaaa     1200

ugcauacguc uuguauugga agaacaaugg uauagcuccc gcaaucaaau ggaaaucuuc     1260

acacaucuaa acauaaaacc aaaaaaaaua aaccuaacag cagcgaacaa aaucccaaag     1320

gcgaugaucg acgaauuuau auuaagucca guggucaaac guacauucgg ucaagcgauc     1380

aaccuaauaa acaaaauaau cgaaaaguac ggaguuccag aagacauaau aauagaacuc     1440

gcgagagaga acaacucaaa agauaaacaa aaauuuauaa acgaaaugca aaagaagaau     1500

gaaaacacac ggaaaagaau aaacgaaaua auuggcaagu auggaaacca gaacgccaaa     1560

agacuaguag aaaaaauaag auuacaugac gaacaagaag gaaaauguuu auauucauua     1620

gaaucaaucc cccuagaaga cuuacucaau aauccaaauc auuaugaagu ugaucacauc     1680

auaccaagau caguaucauu cgauaacucc uaccauaaca aaguauuagu aaaacaauca     1740

gaaaauucga aaaaaucuaa uuuaacaccg uaccaauacu ucaacucagg caaaucaaag     1800

uuaucauaca accaauucaa acaacacaua uuaaaccuaa gcaaaaguca ggacagaaua     1860

ucaaaaaaga aaaaagaaua ucuauuggaa gaaagagaua ucaacaaauu cgagguacag     1920

aaagaauuua uaaauagaaa ucucguugac accagauacg caacacggga acuaacaaau     1980

uaucuuaaag ccuacuucag cgcaaacaau augaauguaa aaguaaaaac uauaaaugga     2040

ucauucacag acuaucuaag aaaaguaugg aaauuuaaaa aagaaagaaa ccauggauac     2100

aagcaccaug cugaagaugc ccuaaucaua gcuaacgccg acuuuuuauu uaaagaaaac     2160

aaaaaacuua aagccgucaa uuccguauua gaaaaaccug aaauagaauc uaaacaacug     2220

gauauccaag ucgacucaga agacaacuac ucagaaaugu ucauuauccc uaaacaagua     2280

caagacauaa aagacuuccg uaauuuuaaa uacucacacc gagucgacaa aaaacccaau     2340

cgccaacuaa uaaaugauac acuguauuca accagaaaaa aagacaacuc aacuuacaua     2400

guacaaacaa uuaaagacau cuacgcgaaa gacaacacaa cacuaaaaaa acaauucgau     2460

aaaucacccg aaaaauuucu aauguaccag caugaccccc gcacauucga aaaauuagaa     2520

gucaucauga aacaauacgc aaacgaaaaa aacccacugg cuaaauacca cgaagagaca     2580

ggcgaauauc uaacaaaaua uuccaaaaaa aacaaugguc caauagucaa gucccuaaaa     2640

uauauuggaa acaaacuagg aucucaucuc gacguaacgc accaguuuaa aucaagcaca     2700

aaaaaacuug uaaaacuaag cauaaaacca uacagauuug acguauaucu uacugacaaa     2760

ggauacaaau uuaucacgau aucauaccua gauguacuca aaaaagacaa cuacuacuac     2820

auaccagaac aaaaauacga uaaacuaaag cuaggcaaag caauugacaa aaacgccaaa     2880

uucauagcau cauuuuauaa aaacgaccua auaaaacucg acggugaaau uuauaaaaua     2940

auuggcguca auagugacac aagaaacaug auagaacugg aucugccaga cauacguuac     3000

aaagaauacu gcgaauuaaa caacauuaaa ggagaaccua gaaucaaaaa aacaauaggc     3060

aaaaaaguca acucaauuga aaagcuaaca accgauguuc uaggcaacgu auuuacaaau     3120

acacaauaca caaaaccgca acuccuuuuu aaaagaggua acuga                     3165


<210>  4965
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4965
augaaauuga caccuuugaa uuauauacuu ggauuagauu uaggaauugc uucaguugga       60

ugggcuguug uagaaauaga ugaacaagaa aauccuuuag gccuuaucga uguaggagua      120

cgcacguuug acagggcuga aguuccaaaa acuggagaau cacuagcacu ugcucgaaga      180

cuagcacggu cagcuaggag auuagucaaa aggagagcag acagaauaaa gaaagcaaaa      240

agauuacuaa aagcggaaaa uauacuacua agugcugaug aacaccuucc aaaugaugua      300

uggcaauuaa gaguuaaagg ucucgaccaa aaauuggaaa gacaagaaug ggcggcagua      360

uuacugcauu uacuuaaaca ccggggauac cugucacagc guaaaaacga aagcaaaucg      420

gaaaacaaag aacuuggcgc acugcuuagu ggcguagaaa cuaaucauca gaucuuacaa      480

ucagcagagu auagaacacc agcagaaaua gcuguaaaga aauuucaugu agaggacgga      540

cacauuagaa accagcgagg agcguauaca cauacauuuu cacgacuuga uuuacuugca      600

gaaauggaau uauuauuuca aagacaaaca gacuuaggaa auccucauac uucugcaaaa      660

uuauuagaaa accuaacagc uuuauuaaug uggcaaaaac cagcauuagc aggcgaagca      720

auauuaaaaa ugcucggaaa auguacauuu gaaccaacug aauauaaagc agccaaaaau      780

agcuauucag cagaaagauu uguaugguua accaaauuaa acaaucuuag gauacuagaa      840

caaggagcag aaagagcacu uacagauaau gaacgauucg cccuccugga ucaaccauac      900

gaaaaagcca aauuuacaua ugcacaagca cggacaaugu uagcuuuacc agaugaagcc      960

auuuucaaag gaguucguua ucaaggagag gacaagaaag caguugaaac caaaacgaua     1020

cuaauggaaa ugaaagccua ucaccaaauc cggaaagcau uagaaaaugc ugaucuaaaa     1080

gcagaaugga acgagcucaa aaacaacuca gaacucuuag acgacauagg uaccgcguuc     1140

agccuacaca aaacagauga ggacauaugu agauacuuag auggaaaauu gagugaguca     1200

auauugaacg cauuauuaga aaacuuaaau uuugauaaau ucauucaacu uucacugaaa     1260

gcacuacaac aaauacuucc auuaaugcuu caagggcaga gauacgauga agcaguuuca     1320

gcuaucuaug gagaccauua uggcaaaaag agugcagaaa uaaaccgauu acuaccgacc     1380

auuccagcug acgaaaucag aaaccccguu guccuccgaa cauuaaccca agcaagaaag     1440

guuauaaacg cuguagucag acuuuaugga agcccggcac gaauccauau agaaacgggc     1500

agagaaguag gaaaaaguua ccaagaccga aagaaauuag aaaaacaaca ggaagacaac     1560

agaaaacaaa gagaaucagc aguaaagaaa uuuaaagaau auuucccuaa uuuuguugga     1620

gaaccaaaag gaaaagauau ucuaaaaaug cgcuuauaug aacuacaaca agcaaaaugu     1680

uuauacagug guaaaucacu agaacuucau cguuuauuag aaaaagguua cguagaagua     1740

gaucacgcuc ucccauucag uagaacuugg gaugacaguu ucaauaauaa agugcuagua     1800

uuagcaaaug aaaaccaaaa uaaagguaac uuaacgccau augaaugguu agacgguaaa     1860

aacaauucag aacacuggca aaauuuugua gcuagaguac aaacaagugg auucucacac     1920

acuaagaaac aaagaaucuu gucccacaaa cucgacgaga aaggauuuau cgaaagaaac     1980

uuaaacgaua caagauacgu cgcgagauuu cuauguaacu ucauagcuga caauaugcua     2040

uuaacuggaa aagguaaaag aaaaguuuuc gcuucaaaug gacaaaucac ugcuuuacua     2100

agagguagau ggggacuaca aaaaguucgg gaugacaaug aucgucauca cgccuuagau     2160

gcagucgucg uugcauguuc uacaguagug augcaacaga aaauuacacg auuuguuaga     2220

uacgaagaag gcaauguguu uucaggagaa agaauagaca gagaaaccgg agaaaucauc     2280

ccauugcacu uuccaagucc cugggcauuc uuucgcgaaa augucgaaau aagaauauuu     2340

agugaaaauc caaaacuaga guuagaaaau cgcuuaccgg acuauccuca guauaaucau     2400

gaguuugugc aaccauuauu uguaaguaga augccaacaa ggaagaugac aggacaaggu     2460

cauauggaaa caguaaagag ugcuaagaga cuagacgaag gauuaagcgu auuaaaaguc     2520

ccauuaaccc aguuaaaauu aucagauuua gaacguaugg uuaacagaga acgugaagua     2580

acauuauacg aaagccuaaa agcaagauua gaacaauuug gaaaugaccc ugcaaaagcc     2640

uuugcagaac cauuucauaa gaaaggaggg gcagucguca aagcuguaag aguagaacaa     2700

acacagaaaa gcggaguucu ggucagagau ggaaacggug ucgccgauaa cgcaaguaug     2760

guccgagucg auguuuucac aaaaggagga aaauacuucc uaguaccuau auacacaugg     2820

caaguagcca aaggaauauu acccaacaag gcaguaacag ccaauguaga cgaaauagac     2880

ugguuagaaa uggaugaauc auaccaauuu auauucacaa uguauccaaa ugaucuagua     2940

aaaguuaaac uaaagaaaga agaauuuuuc gguuauuaug guggacuaga ccgagcuaca     3000

ggagcaauug ucauaaaaga acacgacuua gagaaaagua aagggaaaca aggcauauau     3060

cgaauaggag ugaaauuagc acuaaguuuu gaaaaauacc aaguagauga acuagggaaa     3120

aauauaagac cauguagacc aaccaaacga caacacguca gauga                     3165


<210>  4966
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4966
augaaagaaa aauauauuuu agggcuagac cuagguauca cuucgguagg auacgggauc       60

auaaacuucg aaacuaaaaa aaucaucgau gcaggaguac gcuuauuccc agaagcaaac      120

gucgauaaca acgaaggcag aagauccaaa cgaggcagua gaaggcuaaa aaggagacga      180

auacacagac uugaacgugu uaaacuauua uugaccgagu acgaccuaau uaacaaagaa      240

caaauaccaa ccucuaauaa ccccuaccaa auccgaguaa aaggacuauc agaaauauua      300

ucuaaagaug agcuagccau agcccuacuu cauuuagcaa aaagacgugg aauacacaau      360

auaaauguaa gcuccgaaga ugaagacgcu agcaacgaac uaucaacgaa agaacaaaua      420

aauagaaaua auaaauuacu aaaagauaaa uauguaugug aaguacaacu acaaagacuc      480

aaagaaggcc aaauucgggg agaaaaaaac cgcuucaaaa caacagauau acuaaaagaa      540

auagaccaac ucuuaaaagu ccaaaaggau uaccauaauu uggauaucga cuucaucaau      600

caauacaaag aaaucgugga aacacgcaga gaauacuuug agggcccagg acaaggcagc      660

ccuuuuggau ggaaugguga ucucaaaaaa ugguacgaga ugcugauggg acacugcacc      720

uacuucccac aagaacuaag gagcguuaaa uacgccuacu ccgcagaccu cuucaacgcg      780

cuaaaugacu uaaacaaucu cauaauacaa agagauaauu cugaaaagcu agaauaucac      840

gaaaaauauc auaucauaga aaacguauuc aaacaaaaaa aaaaaccaac auuaaaacaa      900

aucgcaaaag aaauuggagu uaacccagaa gauauaaaag gguaccgcau aacuaaauca      960

ggaaccccac aauucacuga guucaaauua uaucaugauc uuaaaaguau cgucuucgac     1020

aaaucaauuu uagaaaauga agccauccuc gaccaaauag cugaaaucuu aacaauauac     1080

caagaugagc aaucaauaaa agaggaacua aacaaacuuc cugaaauauu aaaugaacaa     1140

gauaaagccg aaauugcaaa auuaauuggg uacaacggua cacaccgucu aucacuuaaa     1200

ugcauccauc uaauaaacga agaauuaugg caaacaucca gaaaccaaau ggaaaucuuc     1260

aauuacuuga acauaaaacc aaacaaaguu gauuuaucgg aacagaacaa aauacccaaa     1320

gauaugguca augacuucau ccuuucacca guaguaaaaa gaacauucau acaaaguaua     1380

aacguaauaa acaaaguaau agaaaaauac ggcauaccag aggacauaau aaucgaacuc     1440

gcgcgagaaa acaacaguga cgauagaaaa aaauucauaa auaaucuaca aaaaaaaaac     1500

gaagcaacua gaaaaagaau aaacgaaaua auaggacaaa caggcaauca aaacgcaaag     1560

agaauagugg agaaaauaag auuacacgau caacaagaag gaaaaugccu auacucacua     1620

gaaagcauag cacucaugga ucuacuaaac aauccacaaa acuacgaagu cgaccauaua     1680

auuccaagau ccgucgcauu cgauaauuca auacacaaca aaguacuagu aaagcaaaua     1740

gaaaauucaa aaaaaggcaa cagaacacca uaccaauacc ucaauaguuc agaugcaaaa     1800

uuaagcuaua aucaguucaa acaacauauc cucaacuuau ccaaaucuaa agaucgcauu     1860

ucaaaaaaaa aaaaagauua cuuacuagaa gaaagagaca uaaacaaauu cgaaguacaa     1920

aaggaauuca uaaauagaaa ucuaguagac acccgauaug cuacgcgaga acuaaccucc     1980

uaucucaaag cuuacuucuc agccaacaau auggauguga aaguaaagac cauaaauggc     2040

ucuuucacga accauuuaag aaaaguaugg agauucgaua aauaucgaaa ccacggauau     2100

aaacaucacg ccgaagacgc acucaucaua gcaaacgccg acuuuuuauu uaaagaaaau     2160

aaaaaacuac aaaacacaaa caagauacug gaaaaaccca caauagaaaa uaauacaaaa     2220

aaaguaacag ucgaaaaaga agaagauuac aacaaugugu uugagacacc caaacuugua     2280

gaagauauca aacaauacag ggacuacaaa uucucacacc gcguagauaa aaaaccaaac     2340

agacaacuca uaaaugacac cuuauauagc acucgaauga aggacgaaca ugacuacaua     2400

guacaaacua uaacagacau auacggaaaa gauaacacga accucaaaaa gcaauucaac     2460

aaaaacccag aaaaauuccu aauguaucag aaugauccaa aaacuuuuga aaaauugucg     2520

auaauaauga aacaauacuc agaugaaaag aacccccuug caaaauacua ugaagaaacu     2580

ggagaauauu uaaccaagua cagcaaaaaa aacaacggac caauagucaa aaaaauuaaa     2640

cugcuaggca acaaagucgg uaaccaccua gacguaacaa acaaauauga aaacucaacu     2700

aaaaaauuag uaaaacuuuc cauaaaaaac uaccgcuucg acguauacuu aacagaaaag     2760

ggcuacaaau uuguaacaau agccuacuua aacguauuua aaaaagacaa cuacuacuac     2820

auaccaaagg acaaauacca agaacuaaaa gaaaaaaaaa aaaucaaaga uacagaccaa     2880

uuuauagcau cauucuauaa aaacgaucua auaaaacuga auggugaccu auacaagauc     2940

auuggaguaa acucugacga uagaaacaua auagaacuag auuauuacga uauaaaauac     3000

aaagauuauu gcgaaauaaa caacauaaaa ggcgaaccga gaauuaaaaa aaccauagga     3060

aaaaaaacag aauccauaga gaaauuuaca accgacgucc uaggaaacuu guaccuccau     3120

ucaacagaaa aagcacccca acuaaucuuc aaacgaggcc ucuga                     3165


<210>  4967
<211>  3165
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4967
auggaaaaua aaaauuuaaa uuauaucuua ggauuagacc uuggaauugc aaguguugga       60

ugggccguag uagaaaucga ugaaaaagaa aacccauuaa gacuaauuga uguaggaguu      120

agaacauucg aaagagcaga aguaccaaaa acaggagaau cauuagcauu aucacgaagg      180

cuagcaagau cugcaagaag acuaacccag cgaagagugg cgcgcuugaa gaaagcaaaa      240

agacucuuaa aaucagaaaa cauacuuuug ucgacagacg agagacugcc acaucaagua      300

uggcaguuac gaguagaagg guuagaccau aaacucgagc gacaagaaug ggccgcaguc      360

uuacugcauc uaauaaaaca cagaggcuau cuaagccaaa gaaagaacga aucaaaauca      420

gaaaauaaag aauuaggagc auuauuauca ggaguugaua acaaucacaa auuacuacaa      480

caagcuacau acaggucacc cgcagaauua gcgguaaaga aauuugaagu cgaggaagga      540

cauaucagga aucagcaagg agccuacacg cauacauucu cacgucuaga uuuauuagcc      600

gaaauggaau uauuauuuag ccgucaacaa cacuuuggua auccauucgc cagugaaaaa      660

cucuuagaga acuugaccgc cuuacuuaug uggcaaaaac cagcacucag uggugaagcu      720

auacuaaaaa ugcuaggaaa augcaccuuu gaagacgaau auaaagcagc caaaaacaca      780

uauucagcag aaagauucgu auggauaaca aaauuaaaua accugagaau acaagaaaac      840

gggcuagaaa gagcacuuaa ugacaacgaa cgucuggcau uaauggaaca accuuaugau      900

aaaaauagau uauuuuauuc acaaguaaga ucaauacuca aauuaagcga cgaagcaaua      960

uuuaaaggac uacgauauag uggagaagau aagaaagcaa uagaaacaaa agcagucuua     1020

auggaaauga aggcuuacca ccaaaucaga aaaguacuug aggggaacaa ucuaaaagcu     1080

gaaugggcag agcuaaaagc aaauccaacu cuacuagacg agauaggcac agcuuucagc     1140

cuauauaaaa cagacgaaga uauaagugcc uacuuggcag guaaauuaag ucaaccagua     1200

cuuaacgcuu uacuggaaaa ccuauccuuu gacaaauuua uacaauuguc acuaaaagca     1260

cuauauaaac uccuaccgcu uaugcaacaa ggcuuacggu acgaugaggc uugccgugaa     1320

auauacggcg aucauuaugg aaagaaaacg gaagaaaacc aucauuucuu accacaaaua     1380

ccagcagaug aaauacgcaa uccaguuguu cuacguacac uaacacaagc aagaaaagua     1440

auaaacggcg uaguaagauu auauggaagu ccagcacgaa uacauauaga aacaggacga     1500

gaaguuggaa aaagcuacaa agauagacgc gaauuagaaa aacgacagga ggaaaacaga     1560

aaacaaaggg aaaacgcuau aaaagaguuc aaagaauauu uuccgcauuu cgcaggagaa     1620

ccaaaagcaa aagacauacu caaaaugaga cuauacaaac aacagaacgc aaaaugccua     1680

uacuccggaa aacccauaga auuacaucgc uuacuagaga aaggauaugu cgagguggac     1740

caugcccuuc cauuuaguag aacaugggau gacagcuuua acaauaaagu ucugguauua     1800

gcaaaugaaa accaaaacaa aggaaacuua acuccauuug aauggcuaga cggaaaacac     1860

aauagcgaaa gauggagagc cuucaaggca cucguagaaa ccagugcuuu cccauaugca     1920

aagaaacaac guauacuaag ucaaaaacua gacgaaaaag gauuuauaga aagaaaccuu     1980

aacgacacaa gauaugucgc acgauuuuua uguaauuuua uagcagauaa uaugcacuua     2040

accggagaag guaaaagaaa aguauucgcu aguaaugggc aaauaacagc acucuuacga     2100

agcagauggg gacuagccaa aucaagggaa gauaaugacc gacaucaugc ucuagacgca     2160

guaguugucg caugcucaac uguagccaug caacagaaaa ucacaagauu uguuagauuu     2220

gaagcgggag acguguucac uggagagcga auugaccgag agacuggaga aauuauaccu     2280

cugcauuucc caacuccaug gcaauucuuc aaacaagaag uugagauacg aauauuuuca     2340

gauaauccca aauuagaauu agaaaaccgc cuaccagauc ggccacaagc aaaucacgag     2400

uucguacaac ccuuauucgu aagccgaaug ccuacucgua agaugacagg gcaaggucac     2460

auggaaaccg ucaaaucggc aaaaagauua aaugaaggga ucuccguaau uaaaaugcca     2520

uuaacuaaau uaaaacuaaa agacuuagaa uuaaugguga acagagaacg agaaaaagau     2580

cuauacgaua cucugaaggc uagacuagaa gcguuuaaug augacccugc caaagcauuc     2640

gcagaaccau uuauaaagaa aggaggagcc auaguaaaau caguuagggu agaacaaaua     2700

caaaaauccg gaguuuuagu uagagaagga aauggagucg cggauaaugc gaguauggua     2760

agagucgacg uauuuaccaa gggagguaaa uacuuuuuag uaccaauaua uacauggcaa     2820

guggcaaaag ggauacuucc aaacaaggcg gcaacucaau auaaagacga agaggacugg     2880

gaaguaaugg auaauucugc aacauuuaaa uuuagcuuac acccaaacga ucuagucaaa     2940

uuaguuacaa agaagaaaac aauauuaggc uacuuuaaug gccucaacag agcaaccgga     3000

aacaucgaua uaaaagaaca cgaccuagau aaaagcaaag gaaaacaagg aauauucgaa     3060

ggcguuggaa uaaaacucgc auuaucauuu gaaaaauacc agguagacga gcuagggaaa     3120

aauauucgau uauguaaacc aucgaaacgc caaccuguuc gguga                     3165


<210>  4968
<211>  3168
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4968
augaauaauu acaucuuagg auuagauaua gggaucacgu caguaggcua uggcauaguc       60

gacagcgaua caagggaaau aaaagacgca ggugugcgau uauuucccga agcaaacguc      120

gauaacaaug aaggacgucg uucaaaaaga ggagcaaggc gacucaaacg aagaaggaua      180

caccgcuugg auagaguaaa gcaucucuua gcagaauaua acuuacuaga ucuuaccaac      240

auuccaaaau cuacgaaccc auaccagaua cgaguuaaag gucuaaacga aaagcuauca      300

aaagacgaac uaguuauagc acuccugcac auagcaaagc gaagaggaau ccacaaugua      360

aaugucauga uggacgauaa cgacucaggc aaugaguuau caacaaaaga ccaacucaaa      420

aaaaacgcca aagcacuuag ugacaaauau guuugcgaac uacaauuaga aagauucgaa      480

caagacuaua aaguacgugg agaaaaaaac cgauucaaaa cagaagacuu uguacgggaa      540

gcuagaaaac uucuagaaac acaaucgaaa uucuuugaaa uagaccaaac uuucauaaug      600

agauacaucg accuaguaga aacgcgaaga gaauauuucg aaggccccgg aaaaggcagc      660

ccauucggau gggaaggcaa caucaaaaaa ugguucgaac aaaugauggg ucacugcaca      720

uacuucccug aagaacuaag aagcguuaaa uacgcauaca gcgcagaacu cuuuaaugcc      780

uuaaaugacc uaaacaaccu aguaaucacu cgcgaugaag aagccaaauu aaauuauggu      840

gagaaauucc aaauaauaga aaacgucuuu aaacaaaaaa aaacaccaaa ccuaaaacaa      900

auagcaaaag aaauuggagu auccgagaca gauauaaaag gauacagagu aaauaaguca      960

gguaagcccg aauucacuca auucaaauua uaucaugacu uaaaaaauau uuuugaagac     1020

ucaaaauacc uaaacgacgu acagcugaug gacaacauag cugagauaau aacaauauac     1080

caagacccag aaaguauuau caaagaacua aaucucuuac cagaauuacu cagcgaaaaa     1140

gaaaaagaaa aaauaagcgc auuaucagga uacgcuggaa cacacagauu aagccucaaa     1200

uguaucaauu uacugcuaga ugacuuaugg gagaguucau ugaaccaaau ggaauuguuu     1260

acaaaauuaa accuaaaacc caaaaaaaua gaccuauccc agcaacauaa aauacccaua     1320

aaauuaguug acgauuucau cuuaucuccg guaguaaaac gagcuuucau ccaaucaauc     1380

caaguaguaa acgcaauaau cgauaaauac ggacuuccag aagacaucau aauagaauua     1440

gcaagggaaa acaauagcga cgauagacga aaauuucuaa accagcuaca aaaacaaaac     1500

gcagaaacac gaaaacaagu agaaaaggua cuaagagaau auggaaauga caaugcaaaa     1560

aggaucgucc aaaaaauaaa acuacacaau augcaagaag gaaaauguuu auauucccuu     1620

aaagacauac ccuuagaaga ccuauuaaaa aaucccaauc auuaugaagu agaccacaua     1680

auaccacgau cagucgccuu ugacaacuca augcacaaua aaguucuagu acgcgcagag     1740

gaaaacucga aaaaaggcaa uagaacaccc uaccaauacu uaaauagcuc ugaaaguagc     1800

cuaucauaua augaauuuaa acaacauaua cucaaucuua gcaaaacaaa agacaggaua     1860

acuaaaaaga aaagagagua ccuacucgaa gaaagagaca uaaacaaaua cgacguccaa     1920

aaagaauuca uuaacagaaa ccuaguugau acaagauacg caacaagaga auuaacuucc     1980

uugcuaaaag cauauuucag cgccaacaac cucgacguca aaguuaaaac aauaaaugga     2040

ucauucacaa auuaucucag aaaaguaugg aaauucgaua aagaccggaa caaaggauac     2100

aagcaucacg ccgaagaugc cuuaauaauc gcaaaugcag acuuuuuauu caaacauaac     2160

aaaaaacuua gaaauauaaa uaaaguacua gaugcuccaa guaaagagau agauaaaaaa     2220

cgaguaacgg uacaaucuga agacgaauac aaccaaaugu ucgaagacac ucaaaaagca     2280

caagcaauca aaaaauucga aauaagaaaa uuuucacaua gagucgauaa aaaaccaaau     2340

agacaauuaa uaaaagacac auuauacuca acucgaaaca uagacggcau cgaguaugua     2400

guagaaagca uuaaagacau auacaguguu aacaacgaua aaguaaaaac aaaguuuaaa     2460

aaagaucccc auagacuuuu gauguacaga aaugacccac aaacauucga aaaauuugag     2520

aaagucuuca aacaauauga aagcgaaaag aacccauuug cgaaguauua cgaagaaaca     2580

ggagaaaaaa ucagaaaauu caguaaaaca ggacaaggac ccuauauaaa uaaaauuaaa     2640

uaccuaaggg aaagauuagg acgccauugc gauguaacaa auaaauauau aaacuccaga     2700

aacaaaauug ugcaacuaaa aaucuacucc uacagauucg auauauacca guacggaaac     2760

aacuacaaaa ugauaaccau aagcuauauu gauuuagaac aaaaaucaaa uuacuacuac     2820

auaagcaggg aaaaauauga acaaaagaaa aaagacaagc aaauagacga uucauauaaa     2880

uucauaggau cauucuacaa aaacgacaua auuaacuaca acggugaaau guacagagua     2940

auaggaguaa augauagcga aaaaaauaaa auacaacucg auaugauaga uauauccaua     3000

aaggacuaua uggaacuuaa uaacauaaag aaaacaggag uuauauacaa aacuauagga     3060

aaaaguacca cccauauaga aaaauacacc acggacauac uaggcaauuu guacaaagca     3120

gcaccaccaa aaaaacccca acuaauauuu aaacgaggca uggaguga                  3168


<210>  4969
<211>  3168
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4969
augacgccaa uuucggaguc acucuuauuc ggauuagaug ucggaauuag uucaugugga       60

ugggcuguuu uaaaacacuc ugacacaaca gggcauauag aagcucuugg aucuuggugu      120

uucaaugcac cagaaacaaa aacugaacga acaccaacua accaaauaag gagaucaucc      180

agauuacuua gacgaguagu uagaagaagg cgaaauagaa ugaccgaaau acgaagacua      240

uuugcuagac augguuuaau agauaaugau agaccagaug cacuaagaca accggcuaua      300

gauccauggg agaugcgugg ccaaggauug gauagacaac uaacaccaga agaauucgca      360

guggcacuag cacacauagc uaaacaccga ggauuuaaau caagugccaa aagaguaaaa      420

agcaaugcau caccagauga uaguaaaaug uuggcugcac uugaaaaaac aaaagaacuu      480

ucagcacaau acagaacaau aggagaaaug uuugccagag auuccgccua uguccaaagg      540

cgcaggaaca gagauggacu auucgaccga accauuagua gagaugacuu aaucgaugag      600

gcuaaaaucu uauuugaucg acaaagaaaa uuaggaaauc cugucgcaag cacugaaaua      660

gaagaggcuu uuacuaggau agcauucugg caaaacccaa ugcaagauag uggcguacug      720

gucgcauccu gucccuuuga acccaaggaa aaaagauccu cacgauuuag cccaucauuu      780

gaacgguuua gacuauugug ucgacuuaua aauuugcgag uaacagaugg uccugaagaa      840

aggccauuaa cggaagaaga auuacguuua gcauaugagc acgcaggaaa aacaagaaaa      900

uuaacagcug cugcaauacg aaagcuuaua ggauuaagag augaccaaca cuuuaccaca      960

auaccaccag aacaggaaaa acaagacauc acauccagaa ccggcgagac uuuugcugga     1020

acagcaaccu uuaggagugu auugggagaa agcuuauggc gacaauuacu gccacaucca     1080

gaaugguuag auaaugcagc cuggauuaua aguuuucacg aacuaacaga aacaauaaua     1140

aaaaaacuug gcgaaauagg cuugcccgua gaagcauaug aauuaauaug cgaugcacua     1200

aaaagcgaca aaagcccuuu ugcaaaauuu aaaggcgcau cuuccuuauc ugauaaagcc     1260

gugcgugcuu uaaucccaua ccuacagcag ggacuuacau acgauaaggc uugccaaaag     1320

gcaggauaug aucacgcggc gaguagguuu agugggcgag cuacgauaga uacaaaagcu     1380

aaauuuaacg cucuuauggc agaaguacgu gauucaaucg caaacccaau ugcucgaaaa     1440

gcgcuaucgg aaggaaugaa acaacuaugg gcaaugagaa accggugggg auuaccaggu     1500

gccauuugua uagaauuagc ucgcgacguc ggaaacagcc uagaaaagcg aaaagaaauu     1560

gaacggaauu uaaaaacaac cgcuaaacaa agagcgagag aaagagagga agcaugugag     1620

uuacuaaauu uaaaagaagu aaguagugau auauuauuac gauauagacu cuggaaggag     1680

caaggaggaa gaugcuguua cagugguaaa ucaauaacac cagacaugcu gauggcacaa     1740

gauaaucgaa uacaaauaga ucauauuuua ccauggucaa gauuugggga cgacagcuau     1800

ucaaacaaag gacucugucu ggcgaaagaa aaccaagcga aaaaaaauca aacaccauac     1860

gaauggauuc auggucaagg aacgacagca caaaaagaac aaugggauaa auuuguugcu     1920

gauguagaaa guagaaaaaa cacaaagggc cuaaaaaaac gaaauuucuu acuaaaaggu     1980

ucuaaagaaa uugaggaacg auuccgauca aggaaucuua acgauacgag auaugcugcc     2040

agacuuaugg uagaagcagc uaggcuauua uauccggcag gacaaagagg agagaaggga     2100

ggaacaagaa ggguauucac acgaccuggg gcuuuaacug cagcucuacg acaugcaugg     2160

gggguagaau cacuaaagaa aauagacggu gaacgauuac augauaacag acaccaugcu     2220

uuagaugcag caauugucgc cgcgguauca gaagcugaaa uacaaaagcu gacuaaaucc     2280

uuccaagaau gugaacaaaa aggacuaagc agacccauga gagacguggc accaccaugg     2340

gauggauuuc gagaccaauu aaaagagaaa uaugaaacaa uuuuuguagc acguccagaa     2400

aggcagagag caagaggcca aggacaugaa gcaacaauac gacaaguaag ggaagaagaa     2460

acaggaccag ugauauacga gagaaaagca guaagugcac uaacauuagc agaucucgcc     2520

aaaauuaaag auccagaucg aaaugcuggc uuaauugcga gccuaagaca guggauagaa     2580

gcaggcaaac cggcagauaa accaccacga acacccugcc accaauccgc agcugggcac     2640

gaaauaagaa aagucagauu agcaacaaua aucacaccag cggugccugu acggggaggu     2700

auggcaaaaa gaggagaaau gacucggguu gacauauuca aaguaauagg aaagaaagga     2760

aaaccagaau gguacaugau accaauauac ccacaccaaa uaaugaaucg cagucaaugg     2820

ccugcaccuc ccaacaaagc gguaguagca ggcaaauccg aagcugacug gccuauaaug     2880

ggugcagaag cucaauuuca auuuucauua uauccauguu cguauguaag acucuuuaaa     2940

gaagguacag uaauagaagg guauuucugu ggaugucaca gaucuagagc guauauaaca     3000

cuugcauuaa guacaggagc ugcuccacua caaaaaggca uagguauaaa aacucaagaa     3060

cauauacaaa aauuuucagu aaacagauuu ggagaacucu caccugucaa acaagagacg     3120

agaacauggc acggaguggc guguaccuca ccagaucaac acgauuga                  3168


<210>  4970
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4970
augaaagcac ugaauuauau uuuaggauua gaccuaggaa uugcuagugu aggaugggca       60

guaguagaaa uagaccagaa ugaguuaccu auacgauuaa uagaucuagg aguccgaacc      120

uuugaaagag ccgaaguccc aaaaacaggg gacucauuag cacucgcucg aagauuagcu      180

cguucuucaa gaagauuggu aagaagacga gcaguucgac uauuaaaagc aaaaagacua      240

cucaaacagg aaggaauauu acaagcagau gauuuccaca guagucacuu aaaaccauua      300

cccaauaacc caugggaauu aagaguaaaa ggacucaauc auuuacucga caggaaagaa      360

ugggcugcug uucuauuaca ucuacuaaaa cacagagggu auuuguccca aagaaagaau      420

gaaaguaaaa cagacaacaa agaguuagga gcacuacuga aagguguaag ugaaaacaga      480

caauuacuac aggcacaggc uuucaguaca cccgcagaaa uagcaaucaa gaaauuucaa      540

caagaagaag gacauauaag aaaucaacgc ggugcauacu cacacacauu cgacagaaau      600

gaucuccugg ccgaacuuag guuacuuuuu caaaaacaaa gagaauuuaa uaacccauau      660

acaucucaau cguuagagca agcgauagcu gaacuauuaa uguggcaaaa accagcacua      720

aguggagaag cuauauuaaa aaugcuugga aaaugcacuu uugaaccaag cgaauacaaa      780

gcgccuaaaa acaguuacuc ggcugaauac uuugucuggc uaacuaaacu aaacaacuua      840

agaauacuag agaauggcgc ggaucgggga cuaacagcug aagaaagaca auuacugaua      900

aaucaaccau ucgaaaaagc aaaauuuacg uacgcacaag cacgaaaacu ucuagaacuu      960

ucagaucuag caauauuuaa agguguaaga uacggaaaag auaaugccga gagcgccaca     1020

uuaauggaaa ugaaggcuug gcacgccaua agaaaagcgu uggaaucaaa uggucuaaaa     1080

acagaaugga auggcuuaaa aaccaaacca gaauuacucg auaaaauagg aaccgcuuuc     1140

uccauuuaca aaacugacga ggauauauca caauaucuaa auggcgaauu aaguccagcc     1200

guauugaacg cauuauuaga aaaucucagc uuugauaaau uuauaagacu uagcuuacaa     1260

aguuuggcca aaauacuccc auuaauggaa caaggauuaa gauacgauga ggcuuguaaa     1320

caaguauacg gggaccacua cggaaagaaa gaacaacuug aguuaagguu uuuaccaaaa     1380

auaccaguug aagauauuag aaauccaguu guauuacgaa cauuaucaca agcuaggaaa     1440

guuaucaaug gugucguaag auuauacgga agccccgcaa ggauucauau ugaaacaggg     1500

agagaagugg gaaaaucauu uagcgaacgc agagauauaa guaaaaggca agaagagaac     1560

aggaaugaaa gacaacgagc cguagaaaaa uucaaagagu ucuucccaca uuucguuggu     1620

gaacccaaag caaaagauau auuaaaaaug aggcucuacg aacaacaaca uggaaaaugc     1680

cuauauucag guaaagaauu agacuuaaac agauuacuag aaaaaggaua cguagaaaua     1740

gaucacgcuu ugccauuuag ccgaaccugg gaugauuccu uuaauaauaa aguauuaguc     1800

cuagcgucag aaaaucaaaa caaaggaaau caaacucccu acgaauggcu caacggcaaa     1860

gaaaaugcag aacgauggaa acuauuccag gcuuuaguaa acggaaguag auucccauac     1920

agcaaaaagc aaagaauacu cacuaaauau cuagacgaag auggauucag agaaaggaau     1980

uuaaaugaca cuagauacgu ugcgagguuu cuuugcaacu ucauugaaga uaauaugcaa     2040

uuaguagguc aagguaagaa acgaguauuu gcgucuaacg gacaaauaac ugcacuauua     2100

agaagaucau ggggaauaca aaaaguaaga gaggauaaug auagacauca ugcagucgau     2160

gcagucguag uagcaugcuc aacuagagcu cuacaaaaga aaauaacaga cuauguuaga     2220

uacaaagaaa ugaauguauu cacgggagag aaaauugauc aagaaacagg agaaauaaua     2280

aaauuucauu uuccagaacc auggaaauuc uuccgacaag aagucgaaau aaggguuuuc     2340

ucagagacac cagcagaaga cguaguaaua gcacuuccag auagaccaca agcaaaucau     2400

gaguucauac aaccuuuauu uguuuccaga gcacccacaa gaaaaaugac aggccaaggg     2460

cauauggaaa ccauaaaaag ugcuaaacga cucgccgaag ggcugucagu aaguagagua     2520

ccgcuaaccc aauuaaaacu aaaagaucug gaaaauaugg uuaaccgaga acgcgaaaaa     2580

gcacucuaug aagcacuaaa aguaagacuu gaaaaauuua acaaugaucc agcaaaagcc     2640

uucgcagacc cuuuuuacaa gaaaggcgga caacaaguua aagcuguuag aguagaacaa     2700

guccaaaaau caggggucuu agugagacaa gaaaauggcg uagcggacaa cgcuagcaug     2760

guacguguag acgucuucau aaaaggagga aaauacuucu uaguaccuau auauacaugg     2820

caaguagcaa aaggaauauu accuaaucgc gcagcaacuc agggaaaaga agaagaauau     2880

ugggacauca uggaugaaac agcuauauuc caauuuaguc uauaucagaa ugauuuaaua     2940

aaacucguua ccaaaaagaa aacuauuuuc ggauacuuua acggacuaaa uagagcaaca     3000

uccaacauaa auauaaaaga acaugaccua gacaaaagua aaggaaaaga cggaauauau     3060

cuagaaguag gaguaaaauu agcacuaucu uuagaaaaau accaaguaga ugaacugggu     3120

aaaaauauac aaccaugucg aaguaugaag aaaagaccac ccgucagaug a              3171


<210>  4971
<211>  3171
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4971
augcccaaac agaaaaguac aagauauaga cuggcacuag auuuaggaag uacaagucug       60

ggcugggccg uuuuaagacu aaaugaacaa caugaaccaa acgcaaucau aagagccgga      120

guuagaauau uuaguaaugg aagaaacccu aaagacgguu caucuuuagc uguaaccagg      180

cgagcggcua gggccaugag aaggaggcga gacagacuuc uaaaaagaaa agcgagaaug      240

augaacaaac uuguggaaua cggauucuuu ccugccgacg uagcagaacg aaagaaacua      300

gaaaggauua acccauacga auuacgugca aaaggacuag augcuccacu aacaccggga      360

gaguuugcaa gagcauuauu ucauauaaac caaagaagag gauucaaaag caaccguaaa      420

acugauaaga aagaaaauga uucaggagca cucaaagcag caauuuccca ucuaagacaa      480

caauuacaug aaagaaaagc aaggacagug ggugaauggc uauggcaacg cuugcaagcg      540

ggacuuggca caagagcaag auacagggaa agcaggguug cacaagcgaa uggaaaaaca      600

agaauagaga aaaguuauga ccucuacgua gauagacaaa ugauugccga agaguucgac      660

gcacuuuggg cagcucaagc ugcauuuaau ccucagcaau ucagcgaaca ggcuagagga      720

gaauuacgag auguccuacu guuccaaaga gaucugagac caguaagacc ggguaggugu      780

acauuguuac cugaugaaga acgagcucca uuagcguuac ccucaguaca aagauuccga      840

auauuacagg aagcaaauca ucuccgauua cuacacccag aucuaagaga agaaccacuu      900

ucguuggcac agagagaugc agucguggau gcuuuagaac auaauaguaa acgaacuuuu      960

gaacaacuaa gaaaauugcu uaaacuuaau ggaacaguca aauuuaaccu ugaagacgaa     1020

aaacguaaag aacucaaagg aaacgccacg agcgcuacac uaucgaaaga uggguuguuu     1080

ggcgaaaauu gguggaacuu agaugaagca cuacaagacg caauaguaug gcaauuagua     1140

ucagaacaau cagaagaaaa acuacaaaac ugguuacaag ccaacacagg uguaaaugca     1200

acacaagcag agacuauagc caaugcaggg cuaccggaag gauauggauc acucucauac     1260

aaagcacuug caaggaucau accagcuuua cgugguacgg aaguagcaaa uggaauagau     1320

guaccaagaa cauacgacca agcaguacaa gcugcaggau ucgcccauca uucggacuua     1380

gauucuggau ucgacuucga acacucacau gaugaaguug aacgaauugg agaacgagua     1440

auagcaagua caggagaagu aaaaccagua uuugcauuua aacaauuacc auauuacggu     1500

aaagcauuaa gaagacacgu ugcguuugca aaagaacauc caagaaacga agaagaacga     1560

uauggaaaaa uugcaaaucc aacaguucac auaggguuaa aucaauugag gguuguagua     1620

aaugcauuga uuaggcguua uggacgccca acugaagucg uuguugaacu agcaagagac     1680

cuaaaacaaa guagagaaca aagacaagaa gaacagaaaa ggcaagcuga cuaucagaaa     1740

aggaaugaac auaucagacg agaaauagcg agcaugcaug ggugcgcuga agaaaggguu     1800

agccacgcag auguucaaaa auggauacug ugggaagaau uaaguuuuga cguagcagac     1860

aggaggugcc cuuauucagg gguucaaaua agugcgcaaa uguuauugag uaaucaagua     1920

gaaauagaac acauauuacc cuuuucucaa acacuagaua auaguuuaaa uaaucguaca     1980

guagcaauga gacaagcaaa uaggauaaag ggaaauagaa cuccauggca agcacgagag     2040

gauuucgaaa gacaaggcug gaguuaugau agcauauuac aacgcgcuga acgaaugccg     2100

aaagcuaaac gauacagauu uucaagggau ggauaugaac gaugguuaaa agaagauaaa     2160

gacuuuuuag caagagcauu aaaugauaca agguauuuau cacgugucgc aaaaacauac     2220

auggaauugg ucuguccuuu agguacuaga gucauaccag gacaaaugac ugcccugcua     2280

agggcuaaau uuggauuaaa ugauguuuua gguuuaaacg gagagaaaaa ccgagaugau     2340

cacagacacc augccguaga ugcaugugua auugggguga cagaucaagg ucuucuacaa     2400

agauuugcuc aagcaucagc ggaugcaaga ggaaacggac ucgaaaaacu gguagaaaac     2460

augccagucc caugggcaac cuaucgagau cauguaaaaa gagcuauaca augcaucugg     2520

guuucucaua aaccagacca cgguuacgaa ggacccauga uggaagaaac ugcauauggu     2580

auucgaaaag acggaaguau cagacagaaa accaaagcag augguucaac ugggcgugaa     2640

auuacacauu uaauaaggau aacagaaccu acacaaaauu uaagacacgg agucgaugca     2700

caaggacaac cacuugcuua uaaagguuau guagguggau cgaaugauug cauagagaua     2760

acacagaaug aacaaggaaa augggaagga aauguaauua caacauuuca agcauaugau     2820

aucguucgua gauuaggguc aaaacaaggc ccacgucgac uuagacaucc cacauuagcc     2880

caaacauuag auguauaugg uaaaccaaag aagcuaguga ugagacuaau gauugaugau     2940

uguguccgcc uagaguuaga uggaaauaag aaaacuuuaa gaguaguuaa aauaacagga     3000

aauggucaag uuuuuauggc accaguacau gaagcuaaug uggauaaaag aaauagugau     3060

aaaacagauc cauuugcaua cguaucuaaa uaugccggau cauuucaaaa agcaaaagcu     3120

agaaggauca caauuucucc aauuggggaa cuccaugauc caggauucug a              3171


<210>  4972
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4972
augucugaua aaaguaaaau acuaggauuu gacauaggaa uaacaucuau aggcugggca       60

uacguagaag gagaagaacu gauagacugc ggagugagaa uauucacaaa agcggaaaac      120

cccaaaacaa aagaaucguu agcgcuaccc agacgugaag caagaggagu gagacgaaga      180

cuugcuagaa gaaaaggaag acuaaacgca auaaaacaac ucauuugcaa agaguucgga      240

uugaacuauc aagacuacau agcacaagau ggagaacuac cgaaagcaua ugcuggaaaa      300

cucauaaacc ccuacgaacu aagaagcaaa gcccuaagag aaaaacuuga aaaagaagaa      360

uuagcaagag uaauucucca cauagccaaa cacagaggcu acggaaacaa acaugcaaaa      420

gaaacagacg acgccgaaag cggcaaaauc aagaaagcga uuaguaauaa cgagaaaauu      480

cucaacgaaa aaggauacca aagugcaggc gaauaucucu accaagaauu uuuccaaaaa      540

gaacgacuac aagaagagaa gaaaggcaac aacacaacaa aagaguucaa aaaugugcga      600

aacaaagccg gcgacuacga acacuguauu gcucagacac aaaugcaaaa cgaacuaaga      660

cuuauauuug aaaaacaaag agaauuugga uuaaacuuca gcgaagaauu uaaaaagaaa      720

guauuggaaa ccgcauucuu ccaaagaccc cuaaaagauu ucucggauaa aauuggagau      780

ugcacauuca ucgaaggagc gaaaagagcg ccaaaagaua gcuuaucugc gauagaauuu      840

gucgcauuag gaagaauaau caauacacuu aaaaaucuac aaaacacaag uggagaagug      900

uauuccagag acaaaauaaa cgaaauacuc caaauaguau uagauaaagg agaaauaagc      960

uacaagaaau uaagagaaau acuagauauc gaaaguucag uaagauucaa agacucaaaa     1020

uuagacuaca caaaagaacu uaaagaagcu gagaaaacaa aauuauuuga aagcaaaaac     1080

uuacaaaaau uuaagaaagc acuaggaagu uuugaaggaa uaagcaggga aauacuagau     1140

agcauagcga aagaaauaau gauaaccaaa gauaaagaaa aauuagcaaa agaacucaag     1200

aaauuuccau uagaugaaac ccaaauacaa aaucugagca acuuaaguuu uagucaaaca     1260

auaaaccuau cauuggaagc gcuaucgcaa auacuuccau ucaugagagg cgaaagggaa     1320

gauaaaugcc uaagauauga ugaagccgua gaaaaagcag gccuaaaaga aguagccaaa     1380

aacaaaagua aagacgacuu ccucccacca uuagaaaauu acgaacccua ccucgcgaau     1440

ccagucguag cuagagcccu aucagaauau agaaaaguau uaaaugcacu guuaaggaaa     1500

uauggcaaaa ugcauaaaau acacauagag uucacaaggg aaguaggaaa aaguaaagaa     1560

gaacgaaaua aaauagaaaa agaacagaaa gaaaacuucg caaagaacca agaagcacag     1620

aaacagugcg aaauacuugg acugccaaua aacggagcua acauccucaa aaugaaauug     1680

uggcgugaac aacaggaauc augcguauac ucaggaaagg aaauagcuau agacgaucua     1740

aagaacccag caauauuaca agucgaccac auauauccuu acucaagauc auucgacgac     1800

ucuuacaaua acaaaguacu cguuuucuca aaagaaaacc aaaauaaauu gaacaaaacu     1860

ccguuugaag ccuuuggaga aaacaaagau aaaugggaga aaauauuaag ccuugcuagu     1920

aagcuaccga aaccaaagcg acaaagaaua uacaacaugc aauucaaaga uaaagaacaa     1980

ggcuuuuuaa cacgaaauau aaacgauaca ggcuauaucg ccaggcuagu aucucaaugg     2040

accaaaaacu gcuuauccuu ugaaaaacua ucagaagaug aagucuccau agcaggcgaa     2100

aaagguucaa aggugcaugu agaaguaaua agcggaucac uaacagcgau guuaagacac     2160

uauuggggac uaggagauaa aaacagagac aaccacuuac aucaugcagu agaugcaaua     2220

auaauagcgu auucaaacga aaaaguuauu aaagccuuca gcgauuauaa gaaacauaga     2280

gaacaaaaua aagcaaaauu cuaugcgaaa gaaaucgcag aaucagaaua caaaaagcag     2340

aaaguauucu ucgaacccac aaaccacuuc aggcaaaaag uccucgagaa aauagauuca     2400

auauucguaa gcaaaccacc cagaaaaaga gcaagaggag cacuccacga agaaacauuu     2460

uauagcuucg aaagauuaug caaugacaaa gaauaugcga agaaagagaa aauggauuac     2520

ggaggaaaag caggaauaca aaaagcaaua gcccucggaa aaauaaggca aguuggaacc     2580

aagauaguuu caaauggaac aaugguuaga guagacaucu uucaacacaa aucaaaugga     2640

aaauucuaug gaguaccaau auacacuaug gauuuugcac ucgguguacu accaaacaaa     2700

gcaguaguau caggaaagga uaaacaggga guuauaaaag acugguuaga aauggauagc     2760

aacuaugagu ucugcuuuag ccuauuuaaa gacgacuuaa uccuaguaca gaagaaagaa     2820

auggagaaag ccgaauuaug cuauuacaca gacuucaauu cuaauacggc cggcauaaaa     2880

uucgaaaaac acaacaacuu auauguaaau cucaaugaaa augaacgcaa gcuauucaca     2940

agcaaagaag ucguaaaagg aggcaagaaa aacguaugga aagauaaaac uccuaaagaa     3000

auagaacaac ucgagaaaaa cagaaaaauc auuggaggau uuguaaccau agaagaucuc     3060

aaaacuuuug aaaaauauca aguuucaccu uuaggagaag uacaaaaagc agauuucgcc     3120

ccaaggcaag ccauaacucu aaaaucaagu ccaaaacauc gaauaaacaa auga           3174


<210>  4973
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4973
augagcaauc ggguaccaua uagauuggca uuagauuugg gaacaaacag cauuggaugg       60

uguuuacuag acuuaaacgg gaacggugac ccaugcggug uaaggcggau ggggguacga      120

auauuucccg auggaagaga cccuaaguca aaggaaaguu uagccgcgga uaggagauua      180

gcucgaucua ugagaaggag gagagaccga uaucuucgaa gaagaagugc auuaaugaau      240

gcccuagugg cggcaggucu aaugccggca gauagagcaa caagaaaagc auuagagucg      300

gaagauccau gugaacuacg agcaagggca uuauuugaac cauuaacagc ugggcagguu      360

gguagagcau uauuucauuu aaaucaaaga aggggauuua aaucaaaucg uaaggcagcu      420

ggugaagaaa augaagcagg aaagauaaaa gcuggaauaa gugcauuaaa agccgaaaua      480

gaagccagcg gcgaaacuac uuuaggagcu ugguuagcug ccagacaucg aaaaagagag      540

accgugagag cgagacugga cggcgcggga gcaaaagcaa gcuacgaauu uuacccugaa      600

aggggauuag uacaugacga guuccaggcu auuagaacug cacaggcacu ucaucaccca      660

gcaguuacgg cagaacaaug ggauagaguc gaaagcguga uuuucagaca aagaccuuua      720

cggccggucc gaccagguaa auguacguua uucccagacc aagaacgagc accaugggca      780

cuaccagugg cucaagaauu ucgaauuuug acugaaauag guaauuuaaa acuguauaga      840

ccaggcgaag aaccucguga acuuucggcu gaucaacaug cacagcucuu ugcgaaauug      900

ugcagaucgg cggauuugac auuugagaaa auucgaaaag aauuaaaauu ggauccagga      960

cuaaaaguua aucuagaagg agagaaaaga gauagacuua aaggagaugc agucacgagu     1020

gcucucucag cuaagaaacg guuugguccc aaauggaaug cuuuaacucc ggacgacaga     1080

agagauauug ucggaagacu uauagaagca gaagaugaag cagaauuagc aggauggcua     1140

gagaguguuc acggacuucc aagcgagaau gcagcacaua cugcagcaag cccaggauua     1200

ccagacggcu auuguaggcu aucagagaag gcccuaguaa aauuaguacc ucuaaugaga     1260

gaacaaaggc ucagguacga ugaagcugua cuggcauuaa acuugcauca cucagauuuc     1320

cgaccugaag guuuacuuga cgaauuacca uauuauggug aaccacuaga acgauuuaua     1380

gcuuuuggaa guggagaacc cucagaccca ccagaacaac aucuuggaag auuuccuaau     1440

cccacaguac auauuggauu aaaugaacuu agaaaacuug uaaaucgcau aguagaaaga     1500

ugggguaaac cggcagauau uguuguagaa uuagcuaggg auuuaaaaag aucuagagau     1560

gagagagaca ccauccagag agaacaagca cagaaccaac guaaaaacga agacagauuu     1620

gcaaaaauaa aggaauuggg cauagaccca agaccggaua gcaugcuaag gcuacggcua     1680

ugggaagaau ugccgaaagu aggaguuggg cauguaugug uauacacagg ggaaccaauu     1740

gguauuagaa gacuauugga aggugacgua gauauagauc acauucuacc guuuagccgu     1800

accuuaucag acggcgccga uaacaaaauu guauguguac guagagcaaa uagauuuaaa     1860

agggacagaa gcccauggga ggcauuugga guaacagcug uagauggaua ugauugggcu     1920

gcaauagcac aaagagccga agaacugccu aaaaauaaac gguggagauu uuuaccagac     1980

gcaauggauc gauuugaagg ggaacgaggu uucuuagaua gacagcuagu agaaacaaga     2040

cauuuaaguc gaguggccag acaauauuua gcuuccaucg ucgacuuaaa caaaguucgc     2100

guugcaccag gaaaacuaac agccaugcuu agagcacgau ggggucucaa uaaaauucuu     2160

ucagauggaa auuuaaagaa uaggucagau cauagacacc augcaauaga ugcggcugug     2220

uuaggaauga uagaucaaag gcuaguacaa uuaguagcac aagcugcagc aagagcagaa     2280

gcgaaugaac uugaaagacu auuugaugga cucccagauc cauuugacgg guuagcagca     2340

acaucauuug gagaucaagu aagggagaaa aucagaggaa uaacuguaag ucacagggca     2400

gaacacgguu ggcaagggag aaugcauaac gacaccgcuu augguccggu ggaggcuguu     2460

aacggcgucc auacgguagu aaccaggaaa ccucuaucag gacuaacagc aaaagauuuc     2520

ccaaauauca gagauagggu uuuggcgaaa gcacuagaag aucacguagc agcggaagca     2580

gccgcuggua ggagcuuugg agaugcauua ggaucauuua aauggggagg acaaccagua     2640

cgaagaguaa gaauguuaga ugugcuagga aauccuauac caauacaaga uccaucuggg     2700

accguuuuua aaguguauaa aggagauuca aaugccuggu augaaauuuu gagggauccc     2760

agagggagau ggacaggaaa aauaguaagu acuuuugaag cauacagguu acauagagag     2820

ugguuagcag aaaccggagg agauucugga agaggcucag gaguauuuuu agacugggcc     2880

gcaaaaaggg uaguagguga agcaggaaca ccuuuguuua gauuguucaa aggagacuua     2940

cuagcucuug gagaagcucc agauagaaga auaguaagag uugucaaauu cucggaagga     3000

cagguugcac ucgcacuuca uuuagaagcu gguaaucuaa aagccaggga cgcagauaaa     3060

ggugauccau uuaaauauca auauugcagc ccagacucau uaagaaaaca ggcggcaaga     3120

ccagcuggag uagauuuacu aggauauguu caugauaaag gaccgcucga auga           3174


<210>  4974
<211>  3174
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4974
augcgaaggu ugggucuuga caucggaaca aauaguauug guugguguuu aauagaagau       60

gacaagaaaa uagucgacau aggagugaga aucuuuucgg acggacguga cccaaaaagu      120

ggagcguccc uagcuguaga cagaagagau gcucgugcaa ugcgcagaag aagagacaga      180

uauuuaggac gaagaagugc auucuuacaa gcucuaguag uccauggcuu aaugccugcu      240

gacgcagaug aagcuaaauu gguugccgau agagacccau accaauuaag agcaaaagca      300

uuacauguag cucuagagcc auuugaaauu gguagagcau uauuucauuu aaaccaaaga      360

cgaggauuca aaaguaauag gaaagcagau aggaaagcaa acgcagggga agacgguaaa      420

auagcugaag gagcaaaagc ucuagaucaa gcaaugucug aagcaggagu ggauacuuua      480

ggucaauuuu uagcuuuaaa agaaacuaaa agagugcgcu uaggaaguga uggucaagca      540

uaugauuucu auccacaaag aagacacaua gaauaugaau uugauaggau auggaaagua      600

caaucugaau accacccugc cuuacuuaca gaagcggcca gagcagcacu acacagaaua      660

cuauucuuuc aaagaccuuu aaaagcacaa gaaguaggau uuuguacuuu ugccggaguc      720

aaugguauag cagcaaauga acggcgucua ccaaaagcac acccacuguu ucagcagaga      780

agauuauacg aagaagucaa ucaauuacgu auuguaucug caggagcagc agauagacca      840

uuaacuuuag acgagagaga uaggcuaaua uuacgacuac aagauagaaa gaaaguuagc      900

uuugauacgu uagcaaagaa agauuuaaga cuugcagaug gcgaaagauu uaauaaagaa      960

ucagaaaaua gaaaagaguu aaucggagac gaaguacgag cagaaauguc agauaagaaa     1020

agguuuggga caagaugggu acacuuugga cuagaugagc aauuaaggau auuagcaaaa     1080

uuacaagaug aagaaaaccc agaggcacua cuagauuggc ugaggaguga auacggucug     1140

gaugaagcug cagcagaauc aguugcuaau gccagauuac cagaaggaua cggaagauuu     1200

ggagaaacag cgacacgucu ucuauuagau gcccuaaaag ccgaagugau aacuuaugac     1260

aaagcagcau ugagagcuuu ugggcaucac agcgauuucc guacggguga gguacuagau     1320

cgccuaccau acuauggaga auuacuaacc agagagauag caccaggaaa accugaauau     1380

ggagauccau uagaacgaca auaugguaaa auaacuaauc caacuguaca caucggguug     1440

agacagcuag aaaaauuagu uaacgcaguc auagcugugc acggaagacc ugaucaaaua     1500

guaguagaau uagcgcgaga gcucaaacuu aaugaaaaag aaaaagauga gcauaaacgg     1560

agaauaaaaa gagauacuga ugcagcaaua gcuagaggga gaaaacuagc aucagaaggg     1620

uuuaucgaua acggagcaaa ucgaauguua augagaauau gggaagaguu gaauccagcu     1680

aauccauuag acagaagaug uccauauugc gcagaaccca uaggaauaag agcacuuuuc     1740

ucgggagaag cagauauaga ucauauaaua ccauauagca gaaguuugga ugauaacgua     1800

gguaauaaag ucguagcaca uagaaguugu aaccgagcaa aagggaauaa aacaccauau     1860

gaaaaauggg guccacacau agauccagaa agaugggaaa gaauuucaga ucaaguagcg     1920

agguuacaua aaucgaaaca auggcgauuu ggaccagacg cgauggaaag aauagaucga     1980

gacgguggau uuuuagcaag acaauuaaca gauacccaau aucuuucgag gguagccgga     2040

aaauaucuca gaagucuaua ucccacagca caaaguggaa gaguugaugu aauaccuggu     2100

agaaugaccg cuauguuaag aagaaaaugg gggcuaaacu cauuacuucc agaccauaau     2160

uacguggaaa augaacacuc aaaugcaccc aaaaaucgau uagaccaucg ucaucacgcg     2220

auagaugcug cgguugcagc cguuacaacu uugucauuaa ugcaacaaau agcagaugcu     2280

gcaggacgcg cagaagacaa aaauuuagau cgacucuucg aagauauacc ccagccuugg     2340

ccaggauuua gagaagaauu aggcgaggca uuaagaucaa cuacgguaag ucacaaagcg     2400

gaccacggga gaaaacgacg uccuuccagg gauagagaug ucacggcugc uagacuacau     2460

aaugauacag cauaugguuu aacaggugau guagcagcgg acggaaaaac gccaauugua     2520

guucauagag ugcccuuagc uagcuuaaaa ccugcugauu uaauugaccc guugaggauu     2580

ccugauccag uucucagaga cgccuuauau gcagcaacac gugaccgaac aggaaaagaa     2640

uuugaacaag cccuaacgca auuugccaga caucacccac uauuuaaagg uauaaggaga     2700

gugagaguaa gggaaccccu aaauguaauc ccgauacgag acgcggcagg cagggcauau     2760

aaagcauaua aaggugacgc uaaugcacga uuugaugucu ggagacuacc aaauggaaaa     2820

uggguuucag auauaguauc cauguuugau gcacacugca uggcugacaa auccgauaga     2880

aggccacacc cggcugcaaa gaaaguacua ucacuaagac aaaacgauau gguagcaauu     2940

gaaagaucgg guguaacaca aauagcccgc guuguaaaau uuucaacgaa cgguaguaua     3000

gcacuagcug ccgacaacga agcaggagca cuuaaagcac gugaugcugc uccaaacgac     3060

guggauccau uuaaauauau auuuucauca gguggaggac uuaaaaacuu aaaagcuaga     3120

cagguaagag uugacccauu aggaagagua uuugacccag gaccaaggga auga           3174


<210>  4975
<211>  3177
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4975
augaaaaaua guuacauacu uggacuagac gucggaaucg caaguguagg auacggcuua       60

auacauucag acucaaaauc cguaauagac gccggaguac gacuauuucc agaagcaaau      120

guagaaaaca acgaggguag acgagcuaaa agaggcagcc gacgacuaaa aagacgcagg      180

auacauaggc uagauagagu uaaauacuua cuugcacaau accaccugau aauugacgac      240

aaaauaccaa aauccacaaa uccuuaugcu auccgaguaa aaggauuauc aucagcacuu      300

uccaaaagcg aguuaguaau agcauuacua caucucgcaa aacgaagagg cauccauaac      360

guaaacguag ucauggacga aaacgaaucg accaacgaau uaucaacaaa agaacaauua      420

aaagaaaacg cuaaacaacu agaaaauaga uacguaugcg aacuacaauu agaccgauua      480

aaugaacaau acaaagucag aggcgaaaga aacagauuca aaacagaaga cuuugucaga      540

gaagccagac aaauucuaac uacccaacaa aaauaucacg acaucgauga acacuucaua      600

gagcaauaua cacacuuacu agaaacuaga cgagaauauu augaaggacc aggaaaaggc      660

ucccaauacg gaugggaagg agacuugaaa caaugguacg aaauguuaau ggguaggugc      720

acauacuuuc cagaagaacu cagaagcguc aaauaugcuu acaccgccga cuuauuuaac      780

gcucuuaacg aucugaacaa ccuaguaauc gcacgcgaug aaaaugaaaa acuagaauac      840

uuugaaaaau accauauaau agaaaacguu uucaaacaaa agaaaagucc aacacuaaaa      900

caaauagcaa aagaaauaga cguuaaagaa ucugacauca aaggcuaucg cauaacuaaa      960

aauggcaaac cccaauucac cagcuucaaa cucuaccacg accuaaauaa aauauuccua     1020

gacaagaaaa aucuagaaaa uauagaacua cuagacgaaa uagcauuuau acuaacacuc     1080

uaccaagauc cccuuagcau uaaagaggcu uuagaccaac uccaagaccu acuaacagaa     1140

ucagaaaaag aagcaauugc acaucucaac ggcuacaccg guacacaccg acucagccuc     1200

aaaugcuuac auuugcugau cgaugaaaug uggcaaacca gccgcaacca aauggaagua     1260

uucacagcau uaaaucuaaa accucaaaaa uaccaacuau cgaaacagaa acaaaucccc     1320

aagaaaaugg ucgaagaauu uauacuuagu ccugucguaa aacgaucuuu uauacaguca     1380

auagaaauag uaaauacaau uauaaagaaa uacggauuac cugaagaaau aauaauagaa     1440

uuagcgaggg agaaaaauuc cgaagacaga agaaaauuuc uuaaccgcau acagaaagaa     1500

aaugaaaaca caagaaaaca aguagaagaa guaauucgcg aauauggaaa cagaaacgca     1560

aaaggauuag ucgagaaaau aaaauugcau caaaugcaag aaggcaaaug ucuauacagc     1620

cuccaaaaca uaccgcuaga ugaccuacua agaaacccau cacacuauga aauagaccac     1680

auaauaccaa gaagcguaag uuucgacaau aguuuuaaua auaaaguccu aguaaaacaa     1740

gaagaaaaca gcaagaaagg caacagaaca cccuaucaau aucuaaacuc aaaugaaagu     1800

guacugucau acguacaauu caaacaacac auucugaauc uuucaaaaag caaagaaaga     1860

aucagcaaaa agaaaagaga auaccuauua gaagaacgug acauaaauaa auuugaaguc     1920

caaaaagaau uuauaaaucg aaaccuaguu gacacaagau augcgacaag agaauuagua     1980

caacuacuaa aaucauauuu cucagcaaau gaccuagacg uuaaagugaa aacaauaaau     2040

ggcucauuca caaacuaccu acguaaagcc uggaaauucg acaaaucucg aaauaaagga     2100

uuuaaacacc acgcagaaga cgcucuaaua aucgcaaacg uuucauacuu auucaaacaa     2160

uccaaagaac uuaaagaagc caacgcaaua cucgaaaaca auaaccucag ucuaaauaaa     2220

aauaaagaua guaaaaaccu uuuaaccgaa caagacuaua gaaaaauguu ugaaauucca     2280

aaacaaguuc aagauauaaa agauuacaaa gacuuuaaau auucacaucg aguugauaag     2340

aaaccaaacc gacaacuuau aaaugacucc uuauauagca caagagaagu aaacgaaaau     2400

cacuacaucg uacagacagu aaaagauaua uaugcaaaag acaacaccaa aauaaaagau     2460

uuauucuuaa aaacuccaga aaaacuauua auguaucaac acgauccuca aacauucgaa     2520

aaacucaugc uagucauaaa acaauaugga gaagagaaaa acccguuugc caaauacuau     2580

gaagaaaaug gugaauaucu uacuaaauau gcaaagaaug aaaaaggucc aacuauaaaa     2640

cgcuuaaaau acauagguaa gaaaguagga acacacuuag acauauccac aaauuucucu     2700

ggggccaaaa gaaaagucgu gcaacucuca aucaaaccgu uucguuucga ugucuuucac     2760

uccaaagcag gauaucacau gcucaccuua acauacaugg auaucaaaaa gaaagaaaaa     2820

cauuaccaaa uacugaaaaa cgaauaccaa gcacuaaaag aaagauacaa agugccauca     2880

gacgcucaau ucauagguuc auucuauuac aaugaucuaa uaucaauaaa ugaugaacua     2940

uucagaguaa uaggaguuaa uaacucaaca agaaacguag ucgaguuaaa uaugguugau     3000

auaaauuaca aggauuauug ugaucucaug ggaauuaaga aaaccccacg aauauucaaa     3060

acuauaacaa agaaaacagg aaaaaucgaa aaauacucca cugauauauu aggaaaccag     3120

uucaaaguaa aaccauccaa aucaccccaa cucauauuca aaaggggugu cuuauga        3177


<210>  4976
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4976
augaggcgac ucggauugga cauugguaca aauucgauag gaugguguuu aacgcaaggc       60

ccgggagaug aagcagacgg aauugucgau auaggaguac guauauuuuc ugacggaaga      120

gauccacaga guggaacuuc guuagcugua gacagaagag augcgagagc cgcaagaaga      180

cgaagagacc gguauauugg uagaaggagc gcauuucugg auacguuagu agcacacgga      240

uuaaugccag cagaugcuga ugaagcuaag cuaguagcag cuuuagaucc auauacucuu      300

agggcccgag cauuagaagc accuuuaaca ccucaugaga uaggucgagc auuauuucau      360

uugaaucaac guagagguuu uaaaucuaau agaaaagcag aacggaaaac uaacgacgcu      420

gacgauggaa aaauagcaac agcagcauau gcguuagaca gggcaauguu agauggagga      480

uuugauacuc uagggcaauu uuuaaaugcu caagaaacaa aacgaguaag aauggaugga      540

gaagcacagg guuacgauuu cuauccucaa aggagacaug ucgaauauga guucgauagg      600

aucuggacag aacaagcaaa acaucaucca gcauuauuaa cagaaacagc gcgguaugca      660

cuacacagga uacuauucua ccaacgaccu cuuaaagcgc aaguugucgg uaaaugcacc      720

uuuguccauu cugaagauag auuagcaaaa gcacaucccu uauuucaaca aagacgauua      780

uaugaagaag uaaaccaauu agaaguuaca acaccaggag aagcuaauag aaaauuaaca      840

cuagacgaaa gagacagauu gguaagagaa cucagccaua agaaagcgau agcauuuacu      900

ucguuagcag caaaaauaaa auuagaaccg ggccaauccu uuaauaaagc gucagaaacu      960

cgaacacaau uagcuggaga ugagguaaga gcaguauuag gggauaagaa aagauucggg     1020

ccagccuggg aacauuuuga cagggacagg caguggacaa uuguugaaag auuauuggaa     1080

gaagaagauc ccgacgcacu ucacgcauuu uuaacccaag acugugguuu agaugaugaa     1140

cacgcugccg cuacugccaa aacaaguuua ccugaaggcu acggaagacu gggagcuaca     1200

gcaacagcga gaauuuuagc agaacuucaa gcggaaguca ucacauauga cgaagcugua     1260

cgccgaugcg gguggcauca uucagaucau agaaccgggg aaauauuacc ucaauuaccu     1320

uacuacggcg aacuauuaac aagggauauc ccuccaggua cacaagaucc aaacgaccca     1380

ccugagaaaa gaugggggag aauaacaaau ccuacagucc auauugguuu aaaccaagua     1440

cgacaauuag uuaaugcuau aguugcagua caugguagac cagaucaaau cguuguagaa     1500

cuagcaagag aauuaaaauu aaaugaaaaa gauaaagaag aacauaaccg aagaaucaga     1560

cgcgauacag cagcagcaau aaaacgguca gaaaaauuaa cacaagaagg uuuuccagau     1620

acaggcgcaa acagaaugcu acuaagauua ugggaagacc uaaauccggc gaacccacua     1680

gacagaagau gcccauauug uggagcccca auuggaauga gagcguuauu ugcaggagaa     1740

gcugauguug aucauaucau acccuacagc cgaaguuuag acgauucagc ugguaauaaa     1800

guuguagcac acagaaguug uaauagagcu aaaggcaaua gaaccccgua cgaaaagugg     1860

ucagacgauc cagauagaug ggacgcaauc accgcacaag uagcucgacu acaccgaagu     1920

aaacaauggc guuuuggacc agacgcaaug gcacgaguag agaaggaugg cggauuuuua     1980

agucgacaau uaacagauac ucaauauuua ucacgauuag cagguaggua uuuaaguagc     2040

uuauacccaa caaaagagga agguagcgua uaugugauac caggacgcau gaccgcuaug     2100

uugaggagau uguggggauu aaacgauaua uuaucugacc acaauauagu ggaaaacaag     2160

cacucuaacg caccuaaaaa cagauuggac cacaggcacc augcuaucga cgcagcagua     2220

guagcuguuu cuacaagauc ccuuaugcaa agaauagcua gaacugcagg acaggcagaa     2280

gaucguaauc uagaccgauu guuugaaggc cuugauccac cauggcccgg cuuuagagau     2340

caauuaagag auagauugcu ggcagucaca guaucacaua aagcagauca cggaagaacg     2400

gggacaccag uacccggaaa agaugcaacu gcaggaagac uacauaauga cacagcauac     2460

gggcucacag gcgaaaccag ugcaaaaggg uuaccaauag uaguacaucg cgucccucua     2520

acaagccuua aaccggcaga caucacggac cccgaucgca uacccgaucc aguacucaga     2580

gacgcaaucg aacgcgcgac agcaggcaaa augggaaaag acuuuacaga cgcucuagcg     2640

aaauucggaa gaaguggagc gcaagagaca gaucgaaaag gagaacgucu auuuagagga     2700

acaagaagag uaagagugag agaaccuuua gggguaauuc cgauagcuga ugccagugga     2760

agaguauaua aagcauacaa aggagacagu aaugcgcgau uugauguaug gcgauuacca     2820

gauggaaaau gggucuccga uauaguuuca auguuugacg cucacagacc cgaccaaggu     2880

gauagaagac cccauccagc agcgaagaaa guauuaaguc uaagacaaaa ugauaugauu     2940

gcaauagagc gugauggagc acccagagaa auuguacgcg uaguaaaauu cucaacuauu     3000

ggaagugugc aacuugcagc aacgacugaa gcaggccccc uaaaagccag agaugcuagu     3060

cccccagacg uagauccuuu ucgauauauu aauucaagcg cgggagguuu aaagaaaauu     3120

aaagcaagac agguccgaau agauccuuua ggccguauau uugauccugg uccgagauga     3180


<210>  4977
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4977
augauagaaa gaauauuagg aguugauuua ggaauaucaa gcuuaggaug ggcuauagua       60

gaauaugaua aagaugacga ggcagcaaac agaaucauag acuguggagu uagguuauuc      120

acagccgcag aaacucccaa aaaaaaggaa ucgccgaaca aagccagaag ggaagcuagg      180

ggcauacgcc gaguauuaaa uagaagaaga guccgaauga acaugauaaa aaaauuguuu      240

uuacgagcug gacuuauaca agauguugac cuggauggag aagguggaau guucuacuca      300

aaagcaaaca gggcagaugu augggaacua agacaugaug gauuauacag acuucugaaa      360

ggagacgaac uggcaagagu acucauacac auagcaaaac auagaggaua uaaauucauc      420

ggggaugaug aagcagauga agaauccgga aaagucaaaa aagcaggagu uguacuaagg      480

caaaacuucg aagcagcagg gugcagaaca gugggugaau ggcuauggag agagagagga      540

gcgaauggaa aaaaaagaaa uaaacaugga gauuacgaaa uaaguauuca cagagaccua      600

cuaguggagg aagucgaggc aauuuuugua gcucaacaag aaaugagauc gaccauagcg      660

acagacgcgu uaaaggcugc auauagagaa aucgcuuuuu ucguaaggcc caugcaaaga      720

auagaaaaaa ugguugguca cuguacauac uuccccgaag aaaggcgagc gccuaaauca      780

gcuccaacag cagaaaaauu uaucgcuauu aguaaauucu uuucaacugu aauuauagau      840

aacgaagguu gggaacaaaa aauaauagag agaaaaacuc uagaggaacu ucuagauuuu      900

gcaguuucaa gagaaaaggu ugaauuuaga caucuucgua aauuucuaga ucuaucagac      960

aacgaaauau uuaaaggcuu acauuauaaa ggaaaaccaa aaacggcaaa aaaaagagaa     1020

gcaacuuugu ucgauccaaa ugaaccaaca gaauuagaau ucgacaaagu ugaagcagaa     1080

aaaaaagcau ggauuaguuu gcgaggagca gcgaaauuac gagaagcauu gggaaacgaa     1140

uuuuaugguc guuucgucgc acuggggaaa cacgcugaug aagcaacaaa aauacuaacu     1200

uauuauaaag augaaggaca aaaacgaaga gaauuaacga aauuaccgcu agaggcagaa     1260

augguagaaa ggcuagugaa aaucggauuu agugacuuuu uaaaauuauc acuaaaagca     1320

auacgagaua uacuacccgc uauggagucc ggagcaagau augacgaagc cguauuaaug     1380

uuaggcguac cacauaaaga aaaaagugca auauuaccac cacucaacaa aaccgacaua     1440

gacauauuaa auccaaccgu aauaagagcu uucgcacaau uuagaaaagu agcuaacgcu     1500

cuaguacgaa aauacggagc auuugauagg guacacuucg aacuggcaag ggaaaucaau     1560

acaaagggug agaucgaaga cauaaaagaa agccaaagga agaacgaaaa agaaagaaaa     1620

gaagccgcgg acuggauagc cgaaacuuca uuucaaguac cauuaacaag aaaaaauauu     1680

cuaaaaaaac gauuauauau acaacaagau ggaagaugug cauauacugg agacguaauu     1740

gaacuagaac gucuauuuga ugaaggcuau ugugaaauag aucacauauu accaagaagc     1800

agaucugcag augacaguuu ugccaacaaa gucuuauguc uggcacgcgc aaaccaacaa     1860

aaaacagauc gaacaccaua ugaaugguuu ggacaugacg ccgcaagaug gaacgcuuuc     1920

gaaacuagaa caagcgcccc aucaaaucgg guaagaacag ggaaaggaaa aauagacaga     1980

cuauuaaaaa aaaauuucga ugaaaauagc gagauggcgu uuaaagauag gaauuuaaau     2040

gacacaagau auauggcuag ggcaauaaaa acauacugcg aacaauauug gguuuucaaa     2100

aacagccaca ccaaagcacc agugcaaguu agaucuggaa aacuaacuag cgugcuacgg     2160

uaucaauggg gucuggaaag caaagauaga gaaucccaca cacaucacgc aguagacgcg     2220

auaauuauag cuuuuagcac acaaggaaug guacaaaaac ucucggagua uuaccgcuuu     2280

aaagaaacac acagagaaaa agaacgucca aaauuagccg uaccauuggc caauuuucga     2340

gacgcaguug aagaggcuac uagaauagaa aacacagaaa ccgucaaaga aggugucgaa     2400

guuaaaagac uacuaauaag ccgaccaccc agagccaggg uaaccggaca agcacacgag     2460

caaacagcua aaccauaccc gagaauaaaa cagguaaaaa auaaaaaaaa auggagacuc     2520

gcaccaauug acgaggaaaa guucgagucc uuuaaagcag acaggguagc aagugcaaac     2580

caaaagaacu ucuaugaaac aucaacuauu ccgagaguag augucuacca caaaaaaggu     2640

aaauuucacu uagugccaau auauuuacau gaaaugguac ucaaugaauu gccaaacuua     2700

ucucuaggaa caaaccccga agcgauggac gagaauuucu uuaaauuuag uauuuuuaaa     2760

gaugaucuaa uaagcauuca gacacaaggu acuccgaaaa aaccagccaa aauuauaaug     2820

ggauauuuca agaauaugca cggagcuaac augguucucu caaguauuaa uaacucaccg     2880

ugugaaggau ucacauguac accuguauca auggacaaaa aacacaagga uaaauguaaa     2940

uuauguccgg aagagaacag aauagcuggc aggugucuuc aagguuuuuu agacuacugg     3000

agucaggaag gauuaagacc accccguaaa gaguuugagu gcgaucaagg aguaaaauuu     3060

gcauuggaug ucaagaaaua ucagauagau cccuuagggu acuauuacga gguaaaacaa     3120

gagaaacgac ucggaacgau cccgcaaaug agaucagcga aaaaauuagu caaaaaauga     3180


<210>  4978
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4978
auguccaaaa gacuaagaua uagauuggcu uuagauuuag ggacuaauuc aauaggauuc       60

ugucuuuugg auuuaaaugg ugacggaaga ccauguggcg ucagacgaau gggcgugcga      120

auuuuuccag auuuuaguaa acuacaacaa ucuuuagccg cagauagacg auuagcaaga      180

ucgaugagaa gaagaaggga ucguuauuua aggagacgca aagcucuaau gaaugcuuua      240

guugcggcug cauugaugcc agcagaucca aaagcaagaa aagcauugga agcgcuugau      300

ccauaugaac ucagagcggc ggcgguggca ggaccacuac cugcccauca guuggguaga      360

gcgcuauuuc auuugaacca aagaagggga uuuaaaagca acagaaaagc agcuggugac      420

gagaaugagg cagguaaagu gaaaucagga auuagugcuc uaaaagcaga acuagcugca      480

agcggggcug gaacacuagg acaguggcuu gcagcuagac augcaaacag acaaccagua      540

cgagcaagau uaacgggaac aggugcaaaa gcaacguaua guuucuaucc agaacgugca      600

uuagugcaug aagaauuuca agcuauuaga gcugcucaag cuccacacca cccgggggua      660

acagcagaac aaugggauag aauagaauca gugauauucc accagcgacc acucaaaccu      720

guaaaaccag gaaaauguac cuuauauccu gaagaagaaa gggcgccaug ggcucugccc      780

aucgcacaaa gauuucguau auuacacgaa cuccacaauu uaaggauagu aaggcccgga      840

caaggugaag agggauuaag uccagcagau uuugauagau ucuuugauag auuauguaga      900

aagggagauc uaacauucaa aacauuggcc aaagaauuaa aauuaccacc agaugcuaga      960

auaaacuugg aagaugauaa acgugacaga uugaaaggag augcuacugc agacgcuuug     1020

agugcagaaa aaggcaaaag aagaagaaua ggagcagcuu ggcauaguuu uccagcggac     1080

aggcaaagag aaauuguagg uagauuaaua gacgcagaag acgaaggcga auuagcugcu     1140

uggcuagucg aggaacuggg ccuaccaugg gaaguagcaa uacuaacugc agaacgaccc     1200

ggcuuaccug acgguuauug ccgauuauca gaaaaagcau uggaacaggu cguaccguua     1260

augcacgaag aacaccuacc auaugaugaa gcaguacgag cacucgguuu acaucauagu     1320

gaucauagaa ccggagaauu ucuagaugaa cuuccguacu auggccuucc auuagagaga     1380

uuuauagcgu uuggaaccgg ggaagcugau gauccgccag aauuaagaau aggaagauuc     1440

ccuaacccua ccguacaugu agcacuaaac gaacuacgua ggguaguaaa cagaauaauu     1500

agaagauggg ggaaaccaga ggaaauugug guagaacuug caagggauuu aaaacaaagu     1560

cgcgcagaca gagauaaaac acaacgagaa caagcggaua aucagaaaaa gaauuggaca     1620

cguacacaac aauuaaggga auuaggaaug gaaccgacag gugaagcucu auuaagguua     1680

agacuauggg aagaauuacc acgaauaggg ucagcucaug uuugugugua uacgggacaa     1740

ccgauaagca ugacgcaacu acugcaagga gauguugacg ucgaucauau auuaccauuc     1800

agcagaacau uagcaaaugg ggcagauaau aaaauaguau gucaucgacg ugcaaacaga     1860

auaaaacgua aucgaacacc cugggaagca uuugguguag uuucggcuga cggauacgau     1920

ugggcugcua ucgcugaaag agcugaauca uugccgaaaa acaaaaggug gcgguuuuug     1980

ccaaaugcaa uggaaagauu ugaaacugaa cgagguuuua uagaccgcca auuggucgag     2040

acaaggcauu uaaguagagu ugcaagggaa uaucuggggu guauaguucc acuagaaaaa     2100

guuagagcag cucccggccg uuuaacagca auguuaaggg gaagaugggg cuuaaauagu     2160

auauuaagug augcaaaucu aaagaauaga ucagaccauc gacaccaugc aguagaugca     2220

gcaguauuag gugugauaga ucaaagauua guacaaagag uagcggaugc cgccaaaaga     2280

gcugaagaga aagaauugga aaagcuauuu gauggacuac cagauccuuu cgaaggacuc     2340

ccagguccuu cauuuagaga ccaaguucaa gaaagggcac aggguauuau uguaagucau     2400

aaaccagaac acggguggca gggaagaaug cauaaugaua cagcauaugg uccaguagag     2460

cgauugcccg acggaacaca uagaguggug acacgaaagc cauuaggguc acuggcagag     2520

aaagauuuug gaaacauaag ggauagagca cuagcagcag cacuugagag acaugucgca     2580

gaacaagcgg cccaaggaag aucauuggga gaggcuuuga gcugcuucac uuggacagga     2640

ggcgaaguua gacgugucag aaugcuagac aaacuagggu cgccuguugc uguacaagau     2700

gaugacgguc auacauucaa acuauauaaa ggagacagua acgcuuggua cgaaauucua     2760

agagauggaa gaggaaggug gacaggacgu auagugucaa cauacgaagc auaucgauua     2820

caugcagaau ggcucgcaga aagaggagac ggggguagau cggaugcagc auucccagau     2880

ugggccgcgc gacgauuaug ugcgggaaua ggaaaaccau uauucagacu auuuaagggc     2940

gacaugcuug cauucggcac agcaccgaau agagaaauca ugaagguagu uaaauuuagu     3000

gaaggaagaa uaguauucgc cggccauaga gaagcgggaa acuuaaaaga aagagaugcu     3060

gauaaagaug auccuuuuaa auauaggacg uguucaccug aagcauuacg aaaggcacaa     3120

gcacgucccg uggguguaga uccagccgga uuuguacaug accgaggccc uuuagaauga     3180


<210>  4979
<211>  3180
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4979
auggguauua gauuugcuuu ugaccugggu acgaacucua ucggaugggc aguauggcga       60

acagggccag gcgucuuugg ugaagauacc gcugcgagcu uagauggaag uggaguacug      120

auauuuaaag auggaagaaa uccuaaagau ggacagucuc uggcgacaau gagaagaguu      180

ccaagacaaa gcagaaaacg uagagacaga uuuguucuua gaagacggga ucuuuuggca      240

gcguuacgaa aagcaggacu auuuccugua gaugucgaag aagguagaag acuagcggcg      300

acugauccau accacuuaag ggcgaaagca uuagaugaga gucugacacc acacgaaaug      360

ggaagaguaa uauuucaucu aaaucaaaga agaggauuuc guagcaacag aaaagcagau      420

cggcaagaua gggaaaaagg uaaaauugcc gaagggucua aacgauuagc agagacacua      480

gcugcaacca acuguagaac uuuaggggaa uuuuuauggu caagacacag gggaacaccu      540

aggacucgau cuccgacuag aauuaggaug gaaggagaag gugcaaaagc acuauaugcc      600

uuuuacccaa cacgagaaau gguaagggca gaguuugaaa gacuauggac agcucaauca      660

agauuugcgc cugaucuacu aacaccggaa cgacaugagg aaauagcggg uauuuuguuu      720

agacaaagag aucuagcucc uccuaaaauu ggauguugua cauucgaacc uucugaaagg      780

agauuaccuc gcgcacugcc uaguguugag gcaagaggaa uuuaugaaag auuagcacau      840

uuacgaauua caacugggcc agucucggac cguggauuaa caagaccuga aagagauguu      900

cuugcaucug cucuauuagc uggcaaaagu cuuacuuuca aagcagugag aaaaacuuug      960

aaaaucuuac cacaugcucu agugaauuuu gaggaggcgg gagaaaaagg ccuagacggu     1020

gcauuaacag caaaguuguu aaguaaaccu gaucauuacg gagcagccug gcaugguuua     1080

ucuuuugcug aaaaggauac auucguugga aaacuccugg augaagcuga ugaagaaaga     1140

cuaauuagaa gauuaguaac agagaauagg cuaagcgaag augcagccag aagaugcgcu     1200

ucuaucccuc uagcggaugg uuauggacgu uuagguagga cagcaaauac ugaaauauua     1260

gccgcauugg uagaagaaac ggaugagaca ggcacgguag uaacauaugc agaagcagua     1320

cggagagcug gagaaagaac aggacgaaac uggcaucaua gugaugaaag ggacgguguu     1380

auuuuagauc gacuuccaua uuauggggaa auccuacaaa gacauguugu uccaggcagc     1440

ggagaaccag aagagaaaaa cgaagcagca cgguggggua gacuugcaaa ucccacaguu     1500

cacauuggau ugaaucaacu uagaaaaguu guaaaccgac uaauagcagc gcacggaaga     1560

ccagaccaaa uagucguaga acuagcucgu gaacuaaaau uaaauagaga acaaaaggaa     1620

aggcuagaua gggaaaauag aaagaauagg gaagaaaaug aaaggagaac agcaauacug     1680

gcagaacaug gacaaaggga uacagcugaa aacaaaauaa gacuaagguu auuugaggaa     1740

caagcaagag cgaaugcugg aauugcauua uguccauaua ccggcagagc uauagguaua     1800

gcugaacuau uuacaucaga aguugaaaua gaccauaucc uaccgguaag ucuaacguua     1860

gacgacagcc uggcgaacag aguauuaugc aggagagaag cgaaucgaga gaaaagacgg     1920

caaacaccau uucaggccuu uggugcaacc ccagcgugga augauauagu ugcuagagca     1980

gcuaaacucc caccuaauaa aagauggcga uuugauccag cagcauuaga aagauuugaa     2040

agagaaggug gguuuuuagg aagacaacua aaugaaacaa aauaucuuuc acgccuggcu     2100

aaaauauacc ugggaaaaau augugaucca gaccguguau acguaacacc uggcacauua     2160

acuggauugu ugagagcaag guggggauua aauagcauau uauccgauuc uaauuucaaa     2220

aaucgaagcg aucaccguca ccaugcaguu gaugcaguag uuauuggggu acuaacaaga     2280

gggaugauac aaagaauagc acaugacgca gcaagagcag aagaucagga uuuagauaga     2340

guauucagag augucccagu gccauucgaa gacuuuagag accauguaag ggaacgagua     2400

agcacaauaa cgguagcagu uaaacccgag cacgguaaag gaggggcacu acaugaagau     2460

acgucauaug gacugguacc agacacagac ccgaacgcug cguugggaaa uuuaguuguu     2520

agaaaaccaa uucguaguuu aacagcuggu gaaguagauc gaguuagaga uagagcacua     2580

agagcaagac uaggagcuuu agccgcacca uuuagagaug agagcggaag aguaagagac     2640

gcaaagggac ucgcacaggc auuagaggcu uucggagcgg aaaaugguau acgaaggguu     2700

agaauccuga aaccagaugc gucaguagua acaauagcag auagaaggac ggggguacca     2760

uaccgagcug uagcacccgg cgaaaaucau cacguagaua ucguacaaau gcgggaugga     2820

aguuggaggg gcuuugcagc auccguuuuu gaaguuaauc gaccaggaug gagaccagaa     2880

ugggaaguaa agaaauuggg uggaaaacuu guuaugagac uacauaaagg agacauggua     2940

gaacuaucag auaaagaugg ucaaagaaga gugaaaguag uacaacagau agagauuuca     3000

gccaauaggg uaaggcuuuc accgcauaac gaugguggaa aacuucaaga caggcaugca     3060

gaugcagaug acccauuuag augggauuug gcgacuauac cauuauuaaa agaccguggg     3120

uguguagcgg uaagaguaga ucccauagga gucguuacac ugagaaggag uaauguuuga     3180


<210>  4980
<211>  3183
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4980
auggcaguaa aaucccuaaa uuauauacua ggucucgauu uaggaauagc aagcugugga       60

ugggcagucg uagaaaugga ugaacaagag aauccuuuaa gauuaauaga cguaggcgua      120

cguacuuuug aagaagcaga gaauccaaaa gacggaucua gccuugcuga aacacgacga      180

cucgcacgag cccaacggcg auugaucuca cgaagagcaa aucgcaugaa caaacuuaga      240

agacuauuaa agcaagaaaa cgugcuccag gcaaaugacu acgacgagaa aggauuaaua      300

auaggauuac cgaaucaagc augggaauua cgagcacaag gauuagauag gaaacuugag      360

ccaaaagagu gggccgcugu auuaauacau uugguaaaac acagaggcua ccuaagccaa      420

agaaaaaacg aaucucaaac agcagauaaa gaacuuggag cacuacuauc ugggaugaau      480

cauaaucauc aacuauuaca aagucaauca aaucaauaua gaacaccugc agaauuagca      540

auaaaacaau uugcaacaca agacggucau auaagaaauc aacgcggagc uuauacccac      600

acuuuuaaca ggcucgaucu ccaaaaugaa cucaaccaau uauucgaugc ccaaacuaga      660

cuugggaacc cacaugucag cggaucauua aaaacagcaa ucaacgauuu auuaaugaau      720

cagaaagcag cacuaucugg cgaugcgauu cuagaaaugu uaggaagaug uacauucgag      780

ccaagugaau auaaagcagc caaaaacacu uacagugccg aacgauuugu cuggcuagga      840

aaauuaacaa acuuaagaau ccaagacaac ggccuggagu cagcucuaag uagagaacaa      900

cgagaauuau ugcuaaauga accuuacaac aaaacaaaac ugacuuauca acaaguacga      960

aaacuacuac gacuaccaga aacugcauuu uucaaaggac uuagauacgg uaaagacaau     1020

gcagaaagug caacacuaau ggaaaugaaa ucauaucacg caauacguaa aucacucgaa     1080

aauaagaguu uaaaaucuga augggagaaa uugaaaacuc aaccaaaauu guuggaccua     1140

auaggaacgg cuuuuaguau cuacaaaaca gacgacgaga uugcaggcug cuuaaaaaac     1200

caauuaccag aaaacguaau cauagccuua caagaacaac uaaauuuuuc gaauuucaug     1260

gaauugucac ucaaagcacu ugagaaacua cuaccucuua uggaacaagg auuaagauac     1320

gaugaagccu gcacacaaau auauggaaau agacaugaaa aaauagcgga caaaacagaa     1380

aaauuccuac caaacauacc cgcagacaaa cucagaaacc cuguuguacu acgaacacua     1440

acgcaagccc gaaaaguaau aaaugcuaua auuagaaggu acggaucucc agcucgagua     1500

cacauagaaa caggaagaga aguaggaaaa ucauuuagcg accgugauaa auuaaaaaaa     1560

caacaggaag aaaaucaaga acaacgacga agagcaaucg acgaauggaa acaauuuuuc     1620

ccgaacgcug cgcacgagcc aaaggcaguu gauauuuuaa aacuacgccu cuaucaacua     1680

caacaaggca aaugucuaua caguggcuua ccaaucgaug uuagacgauu accagaaaaa     1740

gguuaugucg aaaucgacca ugcccuacca uucagcagaa caugggacga cucauuuaau     1800

aacaaaauuc uuguacuagc uuccgagaac cagaauaaag gaaaccaaac accguacgaa     1860

uacuuacgcg gugccgauaa uucagaaucu uggagacuau ucguagaaaa uguacaaaac     1920

ugucaguuuu cacccaauaa aaagcaaaaa auuauggcaa aacaauuaga ugaaaaagga     1980

uuucuugaaa gaaaccuaaa cgacacacga uaugucucaa gauuuuuaug ccaguuuauc     2040

agugaauauc uacaccugac cggaaaagga aaaaaaagag uguuugcaag caauggacaa     2100

aucacagcau uacuucgugg acgaugggga cuuaguaaaa acagagaaga aaaugauaga     2160

caccacgcag uugacgccgu aguaguagca ugcucaacug uagcaaugca acaaaaaaua     2220

acucaauucg ucagauauca aaaaggaaac gucuuuacgg gcgaaagaau agauaaagaa     2280

acuggcgaag uuauaucaau ucauuucccu acgccuuggg agcacuuuag agaagaaguu     2340

augauacgag uauucgauga uaacccuuua agaacacuac cagaaaaacu cccaaauagg     2400

ccugaagccc uacacgaaua uguaaugcca cuauucguaa gccgaaugcc cacaaggaag     2460

augacaggac aagggcauaa agaaaccaua aaaagcgcaa aaagacuaga cgaaaaaaua     2520

agcguacuac gcguuccauu gacacaauug aaauugaacg auuuagaaaa caugguaaac     2580

cgcgagcgcg aaccagccuu guaugaagcc cuaaaagcaa gauuagaaca auuuaaaaau     2640

gacccagcaa aagcguucgc agaaccauuu uucaaacaag gcggucaaca gguuaaggca     2700

guaagaguug aaauaguuca aaagagcggu auguuacuaa gaaaaauaaa ugguguagcc     2760

gauaauggca guaugguaag aguagacgua uuugaaaaau caggaaaguu cuaccucgug     2820

ccaauuuacg cauggcaagu ugcagaagga auuuuaccaa accaugcagu aguacacagu     2880

aaagaugaau ccgacuggau uuuaauggau gaaucauauc auuucaaauu cgcauuguac     2940

aaaaaugacc uacuggaagu ucaaaccaaa aaaggaugua ucaugggaua uuucgcaagu     3000

cuagaccgcg caacaggugc aauaacaauc agagaacacg acagagacaa aaaaaaaugc     3060

aaagacggag aacacagguc aauaggaguc aaaauggcau ccuccuuuaa aaaguuacaa     3120

guggaugaau uagguaaaaa cauucacgaa acaagaagca gaaaacgacc uaauauuggc     3180

uga                                                                   3183


<210>  4981
<211>  3186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4981
augcauaccc uaccauauac ucuaggacua gacauaggaa uugcgucagu aggugccgcg       60

cuauuaagug augaauccau acuuggaaug cacguaagaa ccuuuuccgc agcagaaaca      120

gauaaagaag gagauccauu aaauaaaauc agaagagaag caaggagugc ccguaggaga      180

auacguagga gagcacacag acuacuucga cuuagacgac uucuuaaacg uaccggacua      240

aucagcgaua cgaguuuaga aguacuaaaa acaucuaaaa caccauggca acuaagaagu      300

gaaggacuug aucgacuacu aucccccagu gaacuagcug cugcacucua ucauauagua      360

aaacaucgag gauuucaauc caaucgaaaa gcagaauuaa aaacggacgc aaaggcggga      420

cagaugcuaa guggcguauc agcaaaccaa caauuaauga gagauaaauc auauagaaca      480

auagccgaga ugguauguaa agacgacgca uucaaagacc auaaaagaaa caaagggggu      540

uccuaucgaa acacauuugc uagaucagac cuagccgacg agcuaaaaac acuacuaaag      600

aaacaaagag aauacgacaa uccccuugca accccugagc uagaagaagc aguucauaca      660

cuuuuaaugg caagaagacc agucuuuaaa gacgacgaua uagaaaaacu cguuggauuc      720

uguaccuuug aacauggaga gaaaagagcg ccgaaagcag cauucucuag cgaaagguuu      780

auauggcuug gaaaauuaaa uaacuuaaga uuaauuuauc caggagaaca gaggucauua      840

acuccagaag aaagguccca aauaauuaau uuaccauuua cacaggcuuc aguuaaauuu      900

agucaaauca gaaagaaauu agaucuaccu gaauauguua gauucaacuu agugcguuau      960

aaaccaggug aagaaaccag caaaagcgaa acggcaaccu uuuucgaagc aaaggcauuc     1020

cacgagcuaa ggaaaacuua ucgggagucc gguauaagug aacuuuggga aagagaucaa     1080

caugacccac aacgacuaga ucaaauagca acagcaauag caguauauag agacgaugaa     1140

cgaauuagaa acgaauuauc accucauacu auagaaaacg aagugguaga agccguacua     1200

aauugugauu uuacucaauu cauaaauuua agccuaaaag cguuaaauaa aauacuacca     1260

uauauggaac aaggccaaag auaugaugaa gcauguacau ccgccggaua ccagcaucau     1320

agcccacaac aaaacgaaga gaaaagcggc cgaauacccc cuccugauaa aaaugucaua     1380

agaaacccag uaguauacag aucucuaaac caagcaagga aauuaauaaa cgcaauaaca     1440

aaaagauaug gagcaccagc agccauucau cuugaaaugg cgagagacuu auccaaacca     1500

uucucugaaa gaaaaaguau agaacgagaa caagaaaaau acagaaaaca aaaaguacaa     1560

cuaagaaacg aguacauaga acacuuuggu acagaaccaa auggauuaaa cuuauugaaa     1620

uuuagacucu acaaagaaca agacgcacaa ugcgcuuaca cacaaaaagc auuagaucua     1680

ucaagacuaa acgaagcagg auacguagaa guagaccaca uccuaccaua cagccgauca     1740

uacgaugauu cccucuuaaa caaaacacuu guuuuaacgg aagccaauag agacaaagga     1800

aaccgaacac cauuugaauu uaugggagga gacgaucaaa gucaacacug gcaaaaauuu     1860

guacgguggg uacaaaugaa cccaaaaauu cgucaagcaa aaagaaauag auauuuaaga     1920

aaagauuuuu cagaaaaagc agcuagagaa uuuagggauc gacacuuaaa ugauacacga     1980

uacaucggaa gagaauuaaa agcuaugauc gaaagccaug uacaauggca uccggacuca     2040

aaaaguaaag acuguugugu aguauuauca ggacaacuaa cagcauuauu acgaggacgg     2100

ugggguuuua auaaagacag agcagcgggc gaucuacauc augccguaga ugccgcuguu     2160

guugcugcag caacuagggg uaugauucaa agaauggcaa gacacucaaa auuucacgaa     2220

cuaaagaaug uaaggaccag cuacgcugau ccggacacug gagagguagu aaauuuagcu     2280

gcggcucgag auuuagaaaa acacuuucca uuaccuuggg acaauuuuag aaaugauaca     2340

cuugcuuauc uaaaccccag cgcaauauca gcugaugaau ccgcacaaga agggcauaga     2400

gguguccgag uuucacgggc cccaaccaga aggaaguuag gagccgcaca ucaagaaaca     2460

auuagaucaa aaggcaaaga caacauucuc cuagaacagg gauuaucagc aguaaaaaca     2520

ccuuuaucaa aauuaacuuu gaaaaacaua gaaaacaugg uuggauacga agaucccaga     2580

aacgcagcau uagucaaagu ucuaagagaa agauuagaag ccuucggugg cgauggcgca     2640

aaagccuucg ccccuaauca accaccagua uauaaaccaa gcaaaccagg uaaacaagca     2700

ccagaaauaa gaucgguaaa aauggcauca aaaucuaaag gggcauuaca cguuagggaa     2760

gguuuagcag acucaggaag aaugauacga auagacauau augaaaccaa aaauggcuac     2820

guaggcguac caauauacgu cagugauaaa gccagaagua aauuaccaaa agcauacccc     2880

aaaaaggggg aagauagagc aaauuggcca gagguggaua caaacgacaa cuucuuauuc     2940

ucauuauaua gcacagauug gaucgaagcu aaaacaaaga aagaaacaau agccggauau     3000

guuaaaucgu augacauauu cgauggaaga cuauuaguau augcucauga uaacaagaag     3060

gugguaagga aacuuacacu uagcgcauua gcagaccuac aaaaauuaca uguuucaaua     3120

uuaggagacc ucuauaaggg aggaaccccg aaguguucac cacuuagaaa aggucaagag     3180

uuguga                                                                3186


<210>  4982
<211>  3186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4982
augcuuagau uagguuuaga uauaggcagu acuagcauag ggugguuuuu auacagaauu       60

acaaacggca ugauagcuga aauuauugau ugugguguua gauuauucag ugauggaaga      120

gacgcaaaaa cugggguuag uucagccgua gaacgacgaa acgcuagagc gcuaagaaga      180

agaagagaca gauacuuacg ucguagagcu gcauuaauga gaagauuagc ugcagcaggc      240

cucaugccag aagauccauc acaggcaaaa ccgcucgaac uauuagaccc cuauagacua      300

agagccgaug ccuuggacag aagauuagaa cuuacagaac uaggcagagc acuuuuucac      360

cuaaaucaaa ggagaggauu uaaaucaaac aggcaaacag acagagggaa uaacgaagga      420

ggcaaaauca uagacggaag ugcaagacua gaucaagcua ugauggcugu cggagcucgc      480

acuuaugggg aauuuuugca ucuacgccgu agucaagcag aagauuuacg acguguccca      540

ucaguaagaa cacgacugac acuaagauca guggaugggg gaaaagcuga aacaggguac      600

gacuucuauc cugauaggag acaccuagaa gaagaauuua auaaaguaug ggcagcacaa      660

gcugcacauc acccagucuu aacagacucc cuaagacaug aaguauuuga aacuauauuc      720

uuucaacgac cacugaaagc gccagcaguc gguaggugcc uauuuuuccc agaaccccga      780

cuaccacgug cucaucccuu aacgcagaga agaguacuau augaaacagu aaacgcauua      840

cggauuagaa ccgaaagcgg ugcugcggua ccacugggua aagagcaaag agacaaagua      900

auaaugcuau uagacggaaa agcacccgua aaagccaucg cuucagcuaa auuaucucua      960

aaagcaauug cuaaacaccu gaaauuaagg gauggccaga cauuuacauu agaaaaagga     1020

agggauuaua uagcguguga uccaguaagg gcaucuuuga gccauccgga uagauuuggu     1080

cccagauggu cuguuuuaga agcggaagua caagcugaaa uaguaggaag gauuagagau     1140

gacgaugaua ccauggcauu aguagaaugg uuaaaacaag uucaugguuu agaugaugca     1200

caugcaagag ccaccgcgga ugcaccacuu ccagaaggcu acggaagauu aggagagacc     1260

gcgacaagaa aauuauuaga cugcuuaaaa gcagaaguac uuacuuacuc ggaggcagua     1320

gguaaagcgu uagaucaaca ucauagugau agaagaacag gggaaauccu agaaucucua     1380

ccauauuacg gugaaguauu gcaagcucac guaaucccag gaacuggaga accacaccac     1440

gaugauauca caagguucgg aaggauaaca aauccuacgg uucauauugg gcuaaaucaa     1500

cucagaaggc uugugaauag gauuaucgca cgauacggga aaccagcaga aauuguagug     1560

gaauuagcua gagauuugaa acaaagcaca ggucaaaaga aagcggcaau agcauugaau     1620

uugaaaaaca ggaaagcagc agaggcuaga agugcguugu ugacugaaug gggaguucca     1680

gauacagggg cuaauagaau guacguacga cucuggcaag aauuacauga agaccccaug     1740

augagacgau guccguauac aggaacaauc auaucaccca gccuuuuauu uucuggugcu     1800

auagacauug accauauacu ucccuacuca agaacuuuag augauuccac agcaaaucga     1860

acauugugua ugaaagaagc aaaccgacag aaaagaaaca aaucaccauc agaagcaaga     1920

agagacggua ugagaauuug ggcacuagaa gaugauaauu uaagacauau accggaauuu     1980

aaaagaugga gauuugcaga agaugcuaug gcacgguucg aaggugaaag ggacuuugaa     2040

gcgagagcuc uaguugauac acaauaccuu ucaagaauau cuaaagcaua ucuagaaaca     2100

uuauaugaug gagcagaugg uaaaucucac gugugggugg uacccggaag guuaacugag     2160

auguugagaa gacauugggg ucuuaaugga auacuaacag auccucuucg ugacgugaca     2220

aaagagaaaa aucgaaugga ccaucgucac caugcaauag acgcagccgu uguagcugca     2280

acagaccgag gacugauuaa agcaauggca gaacaagcaa gaacagaugu ugaccaagga     2340

agagaagcuc uagcuucaag uauaaugcca ccuuggccgg gguuuagaga agcaguagcg     2400

gcacaagucg acaggauaau agucagccau aggccagauc auggcagaau agauuuaacu     2460

acgagaauga aagguagaga ucagacuaca ggaccacuac acagggacac ugcauaugga     2520

cuaacaggac aauccgaaaa aggagucccu cuuguuguaa cgagagaacc uuuugauaau     2580

cuaacuccaa caaugauugg aaaaauacga gacgaaccgc uaaaagcagc acuagcugcc     2640

guuacagcug gaaagaaaga ugacgcauuu gcggaagcau uggcccacuu uagagaccga     2700

ccaggaccau accauggcau aagaagagua agauugauug aaccaauauc agucguagaa     2760

auaagagacg caaguggacg cgcauauaaa gguuauaaag gaaacagcaa ccauuguuuu     2820

gaaguaugga gacuaccagg cggugcaauu acagaacaua uaguuucgac guuugaugca     2880

caccaacccg guccugaacc ucgaccucau ccagcugcaa aaagauuauu aagguuauuc     2940

aaaagugaua ugguuaaauu agacgacagc aaauucggac caguaauagc gacuguaguc     3000

auguucgauc ggaacgguaa aauauccuua gugccacaua ccgcaaguaa ugcuaggaaa     3060

agaugugaaa aauauaaaga agauuuauau uuacgauuca cagcuagaac uuuaauagaa     3120

gcuggggcua gaagaauaau agucgaugaa accggacauu uccgagaccc aggaagaaaa     3180

ggauga                                                                3186


<210>  4983
<211>  3189
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4983
auggccgaaa aacaacauag auggggauug gauauuggaa caaacagcau agguugggcu       60

gucaucgcac ucaucgaggg gaggccagcg ggucucguug cgacaggauc acgaauauuc      120

ucagacggaa gaaaucccaa agauggauca ucacuagcag uagaaagaag aggcccacga      180

caaaugaggc gcaggcgaga uagauaucuu agaagaagag aucgauuuau gcaagcguua      240

aucaaugucg gauuaaugcc uggagaugcc gcagcaagaa aagcauuagu aacagaaaac      300

ccauacguuc uaaggcaaag agguuuagac caagcacuua cuuuaccaga auuuggcaga      360

gcauuauucc auuuaaauca aagaagaggg uuucaaagua auaggaaaac ggauagggcu      420

acagcaaaag aaucaggaaa agugaaaaau gccauugcag cauucagagc agguaugggu      480

aaugcccgua caguaggaga agcccuagca agacgauuag aagauggaag gcccguaaga      540

gcgcggaugg uuggacaggg aaaagaugaa cacuaugaau uauauauugc acgugaaugg      600

auugcucagg aguucgacgc auuaugggca ucacagcaga gauuucaugc ugaaguccuc      660

gcagacgccg cuagagaccg uuugagagca aucuuauugu uucaaaggaa auuguuaccc      720

gucccugugg gaaaauguuu ucuagaaccu aaccagccaa gaguagcagc agcauuacca      780

ucagcacaaa gguucagacu uaugcaggaa cuaaaccacc uacgaguaau gacauuggca      840

gauaaacgag aaagaccauu aucauuccaa gaaagaaaug aucuauuggc acaacucgua      900

gcaagaccca aauguggauu ugacaugcua agaaaaaucg uuuucggugc aaauaaggaa      960

gcauauagau uuacgauaga aucagaaaga agaaaagaac uuaaaggaug ugauacugcu     1020

gcaaaauuag ccaaagugaa ugcacuaggg acgagauggc aggcgcuauc auuagaugaa     1080

caagacagac uuguaugcuu acuauuggac ggggaaaaug augcaguauu agccgaugcu     1140

uuaagggaac auuauggcuu aacagaugcu caaauugaca cacuacuagg acuuaguuuu     1200

gaagaugguc auaugaggcu ugggagauca gcacuguuaa gaguacuaga ugcauuagaa     1260

ucaggccgug augaacaagg auuaccacug aguuaugaua aagcugucgu ggcagcaggg     1320

uauccugcac acacagcaga uuuagaaaac ggagaaagag acgcauugcc guauuauggc     1380

gaguuauuau ggcgauauac gcaagaugcg ccaacugcua aaaaugaugc agaaagaaaa     1440

uucggaaaga ucgcaaaccc uacuguacac auaggauuaa accagcuaag aaaauuagug     1500

aaugccuuaa uucaaagaua cggaaagccc gcacagauag uaguugaacu ugcacgaaau     1560

uuaaaagccg guuuagaaga aaaagaacga auuaaaaagc aacaaacugc aaauuuggaa     1620

aggaaugaac gaauacgcca gaaauuacaa gaugcuggug ucccagacaa ucgagaaaau     1680

cgucuaagga ugcguuuauu ugaagaauua ggucaaggaa auggacuagg aacuccaugu     1740

auauauagug gaagacaaau aaguuuacaa agguuauucu caaaugaugu acaaguugac     1800

cacauauuac cuuuuagcaa aacauuggac gauucuuucg caaauaaggu auuagcacaa     1860

cacgaugcaa acagauauaa agguaacagg ggucccuuug aagccuuugg ugccaaucgc     1920

gauggcuaug cuugggauga uauacgcgcc agagcagccg uucuuccaag aaauaaacgg     1980

aaucguuuug ccgaaacagc caugcaagau ugguuacaua augaaacaga uuucuuggca     2040

cgucaguuga cagacacagc auaucuuagc aggguggcaa ggcaauauuu aacggcaaua     2100

uguagcaaag augauguaua cgugagccca gguagauuaa cagcaauguu gagagcuaaa     2160

uggggauuaa auagaguacu agauggagua auggaagaac agggcagacc ugcuguuaaa     2220

aaccgggacg aucaucgaca ucaugccaua gacgcaguag uuauaggagc aacagacaga     2280

gcaaugcuac aacaagucgc cacgcuagca gcacgggcaa gagaacaaga ugcagaaaga     2340

cuuauuggag auaugccuac acccuggccg aacuuuuuag aagauguccg agccgcagua     2400

gcacgaugug uaguaaguca uaaaccagau cauggaccag aaggaggauu acauaaugac     2460

acagcuuaug gaauaguagc aggaccuuuu gaggauggaa gguauagagu uagacauaga     2520

guaucauugu uugaucuuaa accuggagau cucaguaaug uacguuguga cgcaccuuua     2580

caagccgagu uggaaccaau auuugaacaa gacgacgcaa gagcacguga agucgcguua     2640

acagcacuug cagaacgaua uaggcaaaga aaaguauggc uugaagaauu aaugagugua     2700

cuucccauua ggccacgagg agaagauggu aagacacuac cagauagcgc accuuauaaa     2760

gcguauaaag gagauaguaa uuacuguuau gaacuuuuca uaaaugaaag aggccgaugg     2820

gacggcgaau uaauuucaac auuuagggca aaucaagcug cauacagaag guuuagaaau     2880

gauccugcaa gguuucgaag auacacugcu gguggcagac cguuacuuau gagacuaugc     2940

auaaaugacu auauugcagu ggggacggcc gccgaaagga caauuuuccg aguaguaaaa     3000

augagcgaaa acaaaaucac auuggcugaa cauuuugaag gaggaacguu aaaacaaaga     3060

gacgcagaua aggacgaucc uuuuaaauau cuaacgaaaa guccaggggc guuaagagac     3120

cuaggagcgc gaaggauauu cguagacuua auaggucgcg uucuagaucc agguauaaaa     3180

ggcgauuga                                                             3189


<210>  4984
<211>  3189
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4984
augaauuuaa auuauauacu aggucuagau cuuggaauug ccucaguugg augggcagua       60

guagaaauag augaacaaga aaacccacaa agacuaauag auguaggugu uagaacuuuu      120

gacagagccg aaguccuaaa aacaggcgaa ucuuuagcuc uugcucgaag acuagcaaga      180

agcagcagaa gacuuguaaa aagaagggca gaaagaauua aaaaggcuaa gagauuacua      240

aaagcugaaa aucuucuacu aucggcagau gaaauacuuc cuaacgaggc uuggcaauua      300

cgcguaaaag gcuuagauca aaaauuagaa agacaagaau gggcagcagu guuacuacau      360

cuacuuaaac acagggguua ucuaagucag cguaaaaacg aaucaaaaag cgaaaauaaa      420

gaguuaggug cauugcucag cggagucgcg auaaaucacc aaauauuaca aagugcagaa      480

uauagaacuc cggccgagau agcaguaaac aaauuccaag cagaagaggg acauauaaga      540

aaucaaagag gggcguauac acacacauuu ucaagacugg accuuuuggc cgaaauggaa      600

cuauuauucc aaagacaagc aacccuagga aauccacaua cuucgacuaa auuacuagaa      660

aaccuaaccg cauuacuaau guggcaaaaa ccagcauuag ccggugaagc aauauuaaaa      720

auguugggaa aaugcacguu cgaaccaacu gaguacaaag ccgcgaagaa uaguuauagu      780

gcagaaagau ucguuuggcu gaccaaacua aacaauuuaa gaauauuaga acaaggcgcg      840

gaacgcgcac uuacugacaa ugaaagauuu gcauuacuug accaaccaua ugaaaaagca      900

aaacuaacau augcccaagu acgaacucuc uuaggauuaa gcgaugaagc gaucuucaag      960

ggcuuaagau accaaggaga agacaagaaa gcgguagaga caaaaacuac auuaauggag     1020

guaaaagcau accaccagau aagaaaagca cuagaagguu uagauuuaaa agcugaaugg     1080

aaugaauuga aaaauaaucc ggcguuacua gaugaaauag gaacagcuuu cucauuauau     1140

aaaacagacg augacaucca cagauauuua gacggaaaau uaucugaaag cgugcuuaau     1200

gcucucuuag aaaauuuaaa uuuugacaaa uucauacaac uaucccuaaa agcacuacaa     1260

caaauuuuac cuuuaauguu acaaggucaa cgauacgacg aagcaguauc agcaauauau     1320

ggagaccacu auggaaagaa aucggacgaa auugacagau uacucccaaa uaucccaacu     1380

gacgaaauac gaaauccagu aguauuacga acacuaacac aagcaagaaa aguaauaaac     1440

gcaguuguaa gacuauacgg gaguccagcg aggauucaua uagaaacagg aagagaagua     1500

ggaaaaagcu aucaagauag aaagaaacuu gaaaaacagc aagaagaaaa ccgaaaacaa     1560

agagaaaaug cugugaagaa auucaaagaa uacuuuccaa auuuuguagg ggagccaaaa     1620

ggaaaagaua uuuuaaaaau gagacuauac gaauuacaac aagccaaaug uuuauacuca     1680

ggaaaaaguc uggaacuaua ucgcuuacuu gaaaaaggau auguugaagu cgaucaugcu     1740

cuaccauuua guagaacuug ggaugauuca uuuaauaaua aagugcuagu auuagcuaac     1800

gaaaaucaaa auaaaggaaa ugcaacaccc uaugaauggc ucgacgggaa aaauaauuca     1860

gaaggauggc aacauuucgu agcaagaguc caaacuagcg guuucuccua ugcuaagaaa     1920

caacgcguac uuauacaaga acuagaugaa aagucauuua uagaaagaaa ucucaaugau     1980

acgcgauaug uagcaagguu ucuauguaau cauauagcag acaauaugcu ucuaaccggu     2040

aaaggaaaac guaaggucuu ugcuucaaac ggacagauaa cugcacuuuu acgaggaugc     2100

ugggguuuac agaaaguccg agaaaauaac gaccgacauc augcccuaga ugcgguagua     2160

guagcuugcu caacaaaauc gaugcaagac aagauaacga aguucguaaa auauaaagaa     2220

gggagacuuu uuaaagauaa gaaagacgga guuuauaaag gugaaaggau aaauagagaa     2280

acaggugaaa uaauaagccu acacuuuccc accccaugga aauuuuucaa agaaaaugua     2340

gaaauucgca uauucucaga aaacccgaaa cuagaacucg aaaauaggcu accagacuau     2400

ccacaauaua accaugaauu uguacaacca cuauuuguau caaggaugcc gacucgaaag     2460

augacaggac aaggacacau ggaaacuguc aaaucagcca aacgacuuga uaaaggucua     2520

agcguacuga agguaccguu aacacaguua aaacuaucug accuagaaag aaugguaaac     2580

agagaacgug aaauggcacu guaugaagcu cuaaaagaau guuuagaaca auuuggaaac     2640

gauccagcua aagcguucgc agaaccguuc uauaaaaagg gaggagcuuu aguaaaagcu     2700

guacgcguag agcaaacuca aaaauccgga gugcuaguac gcaacggcaa uggcgucgca     2760

gacaaugcca caggaaguau aguaagaguu gauguuuuca cuaaaggagg gaaguacuuu     2820

cuuguaccaa uauacacuug gcaaguugca aaagguauau uaccuaauaa agcaguaauc     2880

caagguaaag augagucaga augggaagaa auggaugaaa acgcgguuuu ccaauucuca     2940

augcacaaaa augaccuuau uaaaguaaaa acaaagaaag gagaaauauu uggauauuuu     3000

aacggacuag auagagcgac aggaaguauu gaaauagaag aacacgaucu agagaaaucu     3060

aaaggcaaaa auggaaguca ucguggacug ggaguaaaaa cagcauucuu cuuggaaaaa     3120

uaucaaguug acgaacuggg caaaaauaua agaccaugua gaccgacaaa aagacaacau     3180

guccgauga                                                             3189


<210>  4985
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4985
auggaaaauc auauaaauua cagacuaggc cuugacauag ggauagccuc agugggcugg       60

agcguuauaa aucucgauaa gaaaagaaua gaagacuuag gaguaagaau uuucaaugcu      120

gcggaacacc caaaagaugg aaguagcuug gcacuuccga gaagaaucgc aagaggaagg      180

cgaagguugu uaaggagaaa ggcauacaga guaaaaagag uaaggaaauu aauuguagac      240

uauaauauau uaacaaaaga ucagcuaaac gaccuauuua aacaaaaaug ucaaaauucc      300

guaugggaaa uaagagucaa agcauuagac aacaaaguaa caaaugaaga augggcuagg      360

cuacugauaa auuuaugcaa aagacgagga uuuaaaagca acagaaagaa cgaagcagau      420

gacaaagaga ccaagaaagu aauaaauagc auaaaaucua auauuaaagg aaugaaagaa      480

aguggcagua gaacaauagg agaauauauc uauacacaaa cccugagcag uggcgauauc      540

ucaaaaucau ucagaaauaa agauggucaa uacaauaauu gcguauccag aaaaaugaua      600

gaggaggaaa uuaaaauacu auuuaagaaa caaagggaac ugggaugcga guucgcuuca      660

gaagaaauag aagaaaaaua ucucgcaaua uucaauucac aaagaccauu uaguaaauuc      720

gaagauuuag agaaaauggu aggauucugu acguuugaaa aagcgagcaa agaaaggaga      780

gccccaaaau guagcauaag cgcugaagaa uuuguauuau acgauaacau gaacaaacua      840

uccauauuaa auaaaggaga uacaagaaaa cuaacuaacg aagaaagaaa aaucaucgua      900

gaaaaugcau ucaaaaacaa agaaauaaaa uauucaaaac uacgaaagau auuaaaucug      960

uccaacgacg aaagauucag cacucuuuca uacagcaaua aaaccgaacu aagcaaaaca     1020

gaaaacagca aauuuguagc auuaaaagga uaccaugaaa uaaaauccgc cauugaaaag     1080

agcuuuggaa aagacacaug gaacaggguc aaaaauaaua ggacaaugcu caaugauaua     1140

gcguauguuc uaagcauagc aaaaaccgac gaagacauaa ucaaacaacu aaguuuaaga     1200

aacauaccua gggaaauaau agaugcuguu uuaggucuau cauuuucaaa auucagcaau     1260

cuaucuauua aggccaucga gaaaauacua ccuuaucuca aagacggaca gcaauauaau     1320

gaagcaugca aaucagcagg auacgacuuc aaagcaacau acaauggaga gaaaagcuau     1380

aaacuccccu uaauagauau agacgaaaua guaaauccag uagugaauag agcacuaagu     1440

caaacaagaa aaguuauaaa cgcaguuaua gacagauaug gcucaccaau aggaauuaau     1500

auagaacugg caagagauuu agccaagaau uuuaaagauc gaaguaaaau caaaaaggaa     1560

caagaagaaa aucgcgcuaa caaagagaaa aucaaaaaug aauuaaaaau guuaaugaac     1620

aaagaaccua ccucaucaga aauacuaaag uacagacucu ggaaagaaca acgagaagag     1680

ugugcauaua gucaagaaag aauauauaua gaagaccucu ucaaaguagg acaguacgag     1740

auagaccaca ucauaccauu uaguaggagc uuugaugauu cuuuaaacaa caaggucuua     1800

guaaaagccg cugaaaacca acgaaaagca aaccgaauac cauaugaaua cuuuggcagc     1860

gaugaagauc gaugguaccg cuucgaagua uggguugaac agucaucacu uagcuucaaa     1920

aagaaaaaua aucuacucaa gaaaagauuu ucccgagaag acagcauaga auuuaaagau     1980

cgaaauuuag uagacacaca auacauaagc agauacauuu caaacuucau aaaaagccga     2040

uuaauauuca aagaaagcga cucaaaacaa aaaguaauaa cgauaaacgg acgagcaacu     2100

ucauaucuaa gagcaaaaug gggauuauua aaaguaagag aaaauggcga caaacaucac     2160

gcauuagacg caacagucau agcgguagca acgcaaggaa ugguucagga gauaucaaaa     2220

uacucgaaag cccaugagcu aaaauacauu agacaaggag acgaguucau agacauagaa     2280

acaggagaaa uaguaaaagu uaaagaccac aaacaccuau caaaagaucg uuuaccagaa     2340

ccauggcuag gcuucagcag cgaacuacga cuccggcuuu cugaaaaucc aauagaagaa     2400

uuaaagaaaa guccgauaag cacauacgau gaagaauuua uacgaaaaac aguaagacca     2460

auauuuguaa guagaguccc cuacagaaaa gucacgggaa gacuauucga ggaaaccgua     2520

uauagcccaa aagccuuuau agacgaaggu uucaucgcca aaaagaaacu acaagauuug     2580

acauuaaaag auuuugaaaa caucuacaau uacaacagug acaagaaacu auacgaugca     2640

uuacgcgaaa gacuagaaga auauggugga aacgcaaaga aagcguucgc agaagaguuu     2700

aggaaaccaa cgaaaacagg aaaauuagga ccuauaguaa aaucaauaaa aguaaaaaca     2760

aaaauuccau ucaaagaugg aauagacuug aauggaggga aagugaagaa agauggucua     2820

guaagaauag acauauacga aaaagaggga aaauacuacg uagugccagu guauagauua     2880

gacauucaac aaggaaauau cccaaagaaa gcugcaguag cuaacaaaga agaaaaagac     2940

uggauacuua uggaugaaag uuauaacuuc aaauuuagcc uuuacaaaaa ugacuuaaua     3000

uacauaaaau acgacuccaa aggagaauac uucggauacu augacggauu cgacagacau     3060

acaggagcca uaacacuaga acgacacgac aacuccgaga gauauagggg aauaggaaua     3120

aaaacgggcg uaaaagagau auuuaaauau aacgucgacg uucuaggacg auauuauaaa     3180

guugcaaaau ga                                                         3192


<210>  4986
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4986
auggaaaagu uucauuaugu ucucggccua gaccugggaa uagcuucagu gggaugggca       60

gcuauugaaa uagauaaaga aacagagaca ucaauuggac uccuagacug cggugucaga      120

acauuugaac gcgcggaggu uccuaagacc ggggauucau uagcgaaagc uagaagagag      180

gcucgaagca caagaagacu gauaagacga cguagccaua gauuauuacg acuaaaaaga      240

cuacuaaaaa gagaaauauu cagacaacca gaaacauuca aagauuuacc aauaaaugcg      300

uggcaacuua gaguaaaagg guuagauuca cgacucaaug aauacgaaug ggcagcugua      360

uuacuacauc ucgucaaaca uagaggcuac cuuucacaaa gaaaaagcga aaugucagag      420

acagacucga aaucagaaau gggccgacuu uuagcgggug uagcagaaaa ccaucaacua      480

cuacaacagg aacaauaucg aacuccggcu gaauuagcuc ugaagaaauu cguuaaacac      540

uucaggaaua aagguggaga uuaugcacau acauucaaca gacuagacuu acaagcagaa      600

uuacaccuac ucuuccaaaa acaaagagaa uuaggaaacc cauuuacaag uccugagcua      660

gaaagacaag uagaugaucu uuuaaugacu cagagaucag cucuacaggg agaugcaauu      720

uugaaaaugc uaggucacug uggcuucgaa ccagaacaau ucaaagcagc caaaaauaca      780

uucucugccg aacgauucau auggcuaaca aaauuaaaua aucuacgaau acaagaccaa      840

ggaaaagaaa gagcauuaac agcagaugaa agaacaaaac uauuagauga accauauaag      900

aaaagcaaau uaacauaugc acaaguaaga aaacuucuau cucucccaca aacagcuauu      960

uuuaaaggau uaagauauga uuuagaacac gauaagaaag cugagaacuc cacucuaaug     1020

gaaaugaaau ccuaccauaa uauaagacaa accuuagaga aaagcggauu gaaaacagaa     1080

uggcaaucaa uagcaacuca acccgaaauc cuugacgcca uagguacagc uuucucaaua     1140

uauaaaacug augaagauau aucccacgag cuuaaaacau guagacuucc agaaaaugug     1200

uugaacgaac uauuaaagaa uauuaauuuc gacggauuua uccaacuauc acuaacugcc     1260

uuaagaaaaa uauugccgcu aauggaacaa ggguauaggu acgaugaagc auguacgcaa     1320

auauauggca aucaucacuc uggaucauua caacaggaau caaaacaauu uuuaccgcac     1380

auaccuauag acgaugucag aaauccagug guauuucgga cauuaacaca agcaagaaaa     1440

gucguuaaug caaucauuag gagguaugga ucgccugcaa gaguacacau agaaauggcg     1500

cgagaauuag gcaaaucaaa aucggauagg gauagaaucg aaaaacaaca acagaaaaau     1560

aagaaagaac gagaaaaugc aguagcaaaa uucaaagaag acuuuccgga cuucguuggc     1620

gaaccucgag ggaaagacau ucuaaaaaug agauuauacg aacaacaaca cggaaaaugc     1680

cucuacucag gacaugacau agacauaaau agacuaaaug aaaaaggcua uguggaaauu     1740

gaccaugcac uaccuuuuag uagaaccugg gacgacucac aaaauaacaa aguuuuagua     1800

cucggaucug aaaaucaaaa caaaagaaau cagacacccg augaauaucu agauggagcu     1860

aauaauucuc aaaggugguu agaauuucaa gcaagaguac aaaccuguca cuucucauau     1920

gguaagaaac aaagaauaca acuagccaaa uuagacgacg aaacggaaaa aggauuccua     1980

gaaagaaauc uaaaugacac ucgauacauu gcacguuuua ugugucaauu cgugcaagaa     2040

aaucuauauc uaacaggcaa agguaaaaga uuaguauuug caaguaaugg gggaaugaca     2100

gcaacauuaa gaaaucugug gggacuaaga aaaguaagag aagauaauga uaggcaucac     2160

gcccuugaug caaucguagu agccugcucu accgcgucaa ugcaacagaa aaucacaaaa     2220

gcauuccaaa gacaugaaag uauagaauau guagacacag aaacaggcga agucaaauuu     2280

agaaucccac agccauggga cuuuuucaga caagaaguca ugauccgugu cuuuuccgau     2340

caaccaugcg aagaucuugu ugaaaaacua agugcaagac cagaagcauu acaugauaac     2400

guuacuccac uauuuguuuc uagagcacca aauagaaaaa uguccggaca aggacaccuc     2460

gaaacaauca aaagugccaa aagauuaagu gaagaaaaca guaugguuaa gaaaccuuua     2520

acuacauuaa aacucaaaga caucccagaa aucguugguu aucccaguag agaaccacaa     2580

cuguacgcag cauugaaaac uagauuggaa acacaugaug acgaucccau uaaagcauuu     2640

gcaaaaccau ucuauaaacc aaacaaaaau ggagaacugg gagccuuagu gagaucagua     2700

agaguaaaag gaguucaaaa cacaggagua augguacaug auggaaaagg aauugcggac     2760

aacgccacaa ugguaagagu agaugucuac acuaaagcag ggaaaaauua ucuaguccca     2820

guauacguau ggcaaguugc gcaaggcaua cuuccuaaca gagcaguuac gagugggaaa     2880

agcgaagcug auugggacuu aauagacgag ucauuugagu ucaaauucag uuugucaaga     2940

ggagaccuag uagaaaugau cucuaacaaa ggucggauau uugguuacua caauggacua     3000

gauagagcaa acgguagcau uggaauaaga gaacaugauu uagaaaaauc aaaaggaaaa     3060

gauggaguac accgaguagg ggucaaaaca gcuacagcau ucaacaaaua ucacguagac     3120

ccucuaggaa aagagauaca uagauguuca agugagccaa gaccgacucu aaaaaucaaa     3180

ucuaaaaagu ga                                                         3192


<210>  4987
<211>  3192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4987
auggaaaaag acuacauacu uggacuagac auaggcauag gcucaguugg uuacggucua       60

auagauuaug acacaaaaag cauaauagac gcuggcguaa gauuauuucc agaagccaac      120

gcugauaaca aucucggaag gagagcaaaa agaggcgcca gaaggcuuaa acgacgccgu      180

auucacagac uagaacgugu caagucccua cuaucagaau auaaaauuau aagcggacua      240

gccccaacaa acaaccaacc cuauaauaua agaguaaaag ggcuaaccga gcaauuaaca      300

aaagacgaac uagcagucgc acucuugcac auagccaaac gacgagggau ucauaauguc      360

gacgucgccg cagacaaaga agagacagca agcgauagcc ucucaaccaa agaccaaaua      420

aacaagaacg caaaauucuu agaaucuaga uacgugugug aacuacaaaa agaacgcuua      480

gaaaacgaag gucacguaag aggaguagaa aacagauucu uaacuaaaga caucguuaga      540

gaggcaaaaa aaauuaucga cacucaaaug caguauuacc ccgaaauaga ugaaacauuu      600

aaagaaaaau acaucucauu aguggagacc agaagagaau acuacgaagg acccggaaag      660

gguucaccau auggauggga ugcagaugua aagaaauggu aucaacuuau gaugggacac      720

uguacguauu ucccugucga auucagaagu gucaaauaug cauacacggc agaucucuau      780

aacgcguuaa augaucucaa caaucuaaca auagcaagag acgauaaccc caagcucgaa      840

uaccacgaaa aauaccauau aauagaaaac gucuucaaac aaaaaagaaa cccaaccuua      900

aaacaaaucg caaaagagau aggagugaau gacauaaaca ucucaggaua ccgagucaca      960

aaaagcggaa aaccgcaauu cacaagcuuu aaauuauuuc augacuuaaa aaaaguagua     1020

aaagaccacg caauacucga ugacauagac cuauuaaacc aaauagcuga aauacuuacc     1080

aucuaccaag acaaagauuc aauaguugca gagcuaggac aacucgaaua uuuaauguca     1140

gaagcagaua aacaaucuau uucagaauua acaggauaca cagggacaca cucacuaucu     1200

cuaaaaugua ugaacaugau aauagacgaa cuauggcacu cauccaugaa ccaaauggaa     1260

guauucaccu auuuaaacau gaggccgaaa aaauaugaac uaaaaggaua ccaaagaauc     1320

ccaacggaua ugauagauga ugcaauccuc aguccaguag uaaaaagauc auucaaacaa     1380

gccauuggag uaguaaaugc aauaauaaag aaauacggac ucccaaaaga cauaauaauu     1440

gaauuagcac gagaaucaaa cucagcagaa aaaagccgau acuuaagagc aauacaaaaa     1500

aaaaacgaga aaacacgaga aagaaucgaa gcaaucauaa aagaauacgg aaaugaaaac     1560

gcuaaaggau ugguccaaaa aauuaaacua caugacgcuc aagaaggaaa augccuauac     1620

ucacuaaaag auauaccucu agaagaccuc cuccgaaacc caaacaacua cgauauugac     1680

cacaucauac cacgaucagu aucauucgac gacagcaugc auaauaaagu acuugugcgg     1740

cgagaacaaa acgcaaaaaa aaauaaccaa acuccauauc aauauuuaac aucgggauau     1800

gcagauauaa aguacucugu uuucaaacaa cacguguuaa accuagcaga aaauaaagau     1860

cgaaugacaa aaaaaaagag agaauauuua cuagaagaac gaaauauuaa caaauaugau     1920

gugcaaaaag aauucaucaa ucgaaaccug guagacacua gauacacaac aagagaacuc     1980

acuacuuuau uaaagacaua uuuuaccaua aauaaucuag acguaaaggu aaaaacaaua     2040

aauggcagcu uuacugauuu ucuacgaaaa agauggggau uuaagaaaaa uagagaugaa     2100

gguuacaaac aucacgcaga agaugcacua aucauagcua acgcugauua cuuauuuaaa     2160

gaacauaaau uauuaaaaga aauaaaagac guaucggacc ucgcaggaga cgaacgaaau     2220

uccaauguaa aggacgaaga ccaauacgaa gaaguauucg gggguuacuu uaaaauugaa     2280

gacauuaaaa aguacaaaau aaaaaaauuu agucauagag uagauaaaaa accuaauaga     2340

caauuaauaa augacacaau auacucuacc agagucaagg acgauaaaag auauuuaaua     2400

aacacacuaa agaaucuaua ugauaaaucu aacggugacc ugaaagaaag aaugcaaaaa     2460

gacccagaga gccuacuuau guaccaucau gacccacaga ccuucgaaaa guuaaaaaua     2520

guaaugaguc aguaugaaaa ugaaaaaaac ccacuagcaa aguauuucga agagacagga     2580

caauaucuaa cuaaauacgc aaaacacgac aauggaccag caauacauaa gauuaaauac     2640

uacggaaaua aacuuguaga acaccuagau aucacaaaaa acuaccacaa uccucaaaau     2700

aaagucguac aacugaguca gaaaaguuuc agauucgacg uguaccaaac agauaaagga     2760

uacaaauuua uaaguauagc auaccuaaca uuaaaaaacg aaaaaaauua uuaugcgauc     2820

ucacaagaaa aauaugauca acuaaaauca gaaaaaaaaa uaagcaauaa cgccguuuuc     2880

aucggaucau ucuauacaag cgacauaaua gaaauaaaua augaaaaauu uagaguuaua     2940

ggugucaacu ccgacaagaa caaccucaua gagguugacc ggauagauau acgucaaaaa     3000

gaauucauag aguuagagga agaaaagcag gucaauuaua ugaacucuga uucauaccug     3060

aagaacaaca gaauaaaagu aacaauagga agaaaaacca caaacauaga aaaauuccau     3120

acagacauau uaggaaacau guacaaauca aaaagaccaa aggcuccaca auuaguauuc     3180

aagaaaggau ga                                                         3192


<210>  4988
<211>  3195
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4988
augucagagu gcgucacgag gcgaauauua ggaauugacu uaggaauugc uaguugcgga       60

uggggaguaa ucgaaguagg agaagcaucc ggaucaauaa uugcuagcgg aguacgaugu      120

uuugacgcuc cuuuaauuga caaaacaggu gagccuaaau cagccacgag aagaaccgcu      180

agaggacaac guagaauaau aaggagaagg agacaaagaa ugaacgcagu ucgaagacua      240

cuagcagagu uuggaguacu uacagggcga ucgccugacg ccuuacacca agcauugcua      300

agguuaaguc aaaguguagc uggaagucag guaacaccuu ggacucuaag ggcggcagcu      360

caugaaagaa aacucacaaa cgacgaauua gcaguaguac uuggacauau agcaagacac      420

cggggauuua gaucaaauag caaaaaugac ggaggugcga augcagccga ugaaacauca      480

aaaaugaaga aagcgaugga gacaacgcga gaaggacuag cacgauauca cagcuuuggc      540

gcgaugauag cgagcgaccc aaaauucgcu gauagaaaaa ggaauagaga uaaagacuac      600

agucacacgg ccaaacguuc agaucuugaa gacgaaguac gcacgauuuu ucgaagucaa      660

acacgguucg guagcuuagu agcaucugaa aaauuaaguc aggcguuugc agacgcagcg      720

uucuuucaac gaccuuuaca agauagcgaa gacauggucg guucaugucc cuuugaacca      780

gggcaaaaac guaccgcaag acgagcaccg ucauuugaac uauuuagauu ccuaagcagg      840

cuggcaaauc ucaaacugac uguuggacga gcaccagaaa gaagauugac accagaugaa      900

auagcacuag ccgcaaaagg uuucggagaa accaagaaau caauaacauu uaaaucauua      960

agagaagcac uagacuuaga uccuaaugca agauuuagug gcguagcaaa agaaaaagag     1020

ucuacauuag auguggcagc aagaacuggc ggagccgcau auggaacuaa aacuuuaaaa     1080

gaugcacugg gggaugcgcc guggagaucu uuaaguagaa ugccugaaaa guuggaccgg     1140

auagcugaaa uauuaucuuu ccgagaagau augaaagcca uaaggaacgg ucuggaagaa     1200

gucgggcuug auggauuagu gguugacgca cuaaugcaag caacggcaaa uggagauuuu     1260

aaagauuuca caagagcagc acacauaagc gcgcuugccg caaggaacau aauuccugga     1320

uuaagagaag gauuaguaua cagcgaugca ugcacaagag uaggauauga ucaugcagca     1380

cgaccggcag ugccccuauc ccaaaucggu uccccaguua caagaaaggc uuuaagugaa     1440

gcauugaagc aagugcgggc gguagcaaga gaauauggac ccauagauua uuuucacauc     1500

gaacuagcaa gaagcaucgg caaaucagcc gaagaaagaa agaaacuaac cgacggaaua     1560

gaagcgagaa auguagagaa agaaaaacga aggaaagaag cugccgagca ucuaggucgg     1620

gcaccaucgg augaugaauu gcuaagauac gaauuagcaa aagaacagaa cuuuaagugc     1680

auauauucag gagauccuau agacccagcc gggauaucug ccaaugacac uagguaucaa     1740

guagaucaua uauuaccgug gaguagauuc ggagacgaua guuaugucaa uaagacacua     1800

uguacugcua gaucaaauca aaacaagcga ggacgcacac cauucgaaug guuugacgca     1860

gauaaaacug aagcugaaug gauggaauau agugcaagag ucgaggaucu aaaagaagua     1920

aagggucgaa agaaaagaaa cuauucuaua aaagaugcgg caucgguaga agacaaauuu     1980

aaagcaagga auuuaacaga cacucagugg gcaacaagac uguuggcaga ugaauuaaag     2040

cgaauguuuc cuccaagaga augugaacga guaguaacug uaagagcaga uggcggaaau     2100

gauggacuau cgauagucga agaaagaaga guauucacaa gaccaggagc aaucacaagu     2160

aaacuuagga gagcaugggg acuagaggga uuaaagaaac aagacggaaa aagaguagag     2220

gaugauagac accacgcagu cgacgcauua gucuuagcgg cuaccacuga aucacuacua     2280

aaucgguuaa caguagaagu acaacagcgu gaacgagaag gacgacaaga cgacauauuu     2340

cauuguaguc aaccauggcc aggguuucga guugacguac agaggacugu auauggaagc     2400

gaaacaaugc caggaauauu cgucucaaga gcagaaagaa ggagggccag aggaaaagca     2460

caugacgcua cugugaaaca aauacgagau auugacggag agagaauagu guucgagcga     2520

aaaccaauug aaaaacucac ugauaaagau uuagaacgua uacccguccc ugaaccauau     2580

ggaaaagcag cagauccuaa aaaguuaaga gaugaauuag uagaaaauuu aagagcuugg     2640

auugccgcug gaaaaccuaa agauaaacca ccaagaaguc cgaaaggaga uauuauuaga     2700

aaaguuagga ucgaaacaaa agauaaaguc gcgguggaaa uaaauggagg aacuguagau     2760

cgaggugaua uggcacgagu agauguauuc cgaaagaaaa auaagaaagg aguaugggag     2820

uuuuacguaa uuccgauuua cccccaucaa auaguagcga gugcccuacc cccaaauaga     2880

gcagugauag ccuauaaagc ugagucagaa uggacugcga uugaugguug uuuugaguuc     2940

gcauggucau uaaauccaau gucuuauuua gaauuaguaa aaaguaaugg ggagcuuaua     3000

gaagguuauu uuagauccau ggauagaacu acuggugcaa ucaauuuauc accgaugucc     3060

accaacagcg aaacuauaag aucaauaggc guaaaaacau uguccagcuu uagaaaauuc     3120

acaguagauc gacuuggucg aaaauucgaa auaccucgcg aaguccgaac uuggagaggg     3180

gaggcaugua cauga                                                      3195


<210>  4989
<211>  3195
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4989
augaaacgaa ccuccuugag agcauauaga cuaggagucg accuuggugc aaauagucug       60

ggaugguuug ucguuuggcu agaugaucau ggucaaccag aaggacuagg accaggagga      120

guaagaauau uuccagaugg uagaaauccc caaucaaaac aaaguaaugc agcugguagg      180

cgacuagcca ggagugcaag acgacgaaga gaucguuacc uucaacgucg aggaaaauua      240

augggucugu uagucaaaca cggguuaaug ccugcagaug aaccagcacg uaaacgcuua      300

gaaugucuug acccuuaugg acuucgagcc aaagcauuag acgaaguacu uccuuuacau      360

cacguaggua gagcauuguu ccaucuaaac caacggagag gacuauuugc caauagagca      420

auagaacaag gugacaaaga cgcaagugcg auaaaagcag cagcaggacg ucuacaaaca      480

ucaaugcaag caugcggagc cagaacauua ggugaauuuc uaaauagaag acaucaguug      540

agagcuacag uacgagcuag aucaccagug gguggugacg uccaagcaag auacgaguuc      600

uauccaacca gagcaauggu agaugcagaa uuugaagcaa uaugggcagc ucaagcccca      660

caucacccaa caaugacagc agaagcgcac gacaccauca gagaagcaau auucucacaa      720

cgagcaauga agagaccaag uauaggaaaa ugcucacuag acccagcgac uucacaagau      780

gacgucgaug gauucagaug ugcgugguca cauccacuug cccaaagauu cagaauaugg      840

caggauguaa gaaauuuagc aguugucgaa acagguccaa caucaucaag auuggguaaa      900

gaagaucagg acaaaguagc uagagcacug cuacaaaccg accaauuaag uuuugacgaa      960

auacggggau uauuaggauu accaucagau gcgagauuca aucuugaauc ugaccgucga     1020

gaucacuuaa aaggggaugc aacaggcgcu auacuuucag ccagaagaca uuuuggucca     1080

gcuuggcacg aucgcucauu agauagacaa auugauauag uugcacuucu agaaucagcu     1140

cuagaugaag cugcuauaau ugcaucucuu gguaccacac auucauuaga ugaagcagca     1200

gcucaacgcg cauuaucagc auuauugccu gauggauauu gucgguuagg uuuaagagca     1260

aucaagcgag uuuuaccauu aauggaagcu ggacgaaccu augcugaagc agccucagcu     1320

gccggauaug accaugcuuu auuaccuggg ggaaaacuau caccaacagg cuaccuacca     1380

uauuaugguc aauggcuaca aaaugaugua guuggaagug augaugaaag agauacuaau     1440

gaaagaagau gggggagacu accaaaucca acugugcaua uagguauugg acaacuacgc     1500

cggguaguaa augaacuaau uagauggcac ggaccgccag cugaaauaac agucgaauua     1560

acaagagauu uaaaauuauc accacgaaga uuagcagaac uugaaagaga acaagcagaa     1620

aaucaacgaa agaaugauaa acgaacaucu uuauuaagaa aacuaggacu accugcaucc     1680

acacauaauu uauuaaaacu aagacuaugg gaugaacaag gcgauguagc uagcgaaugu     1740

ccauauaccg gggaagcaau cggacuugaa aggcuagucu ccgaugacgu cgauauagau     1800

caccuaauac cauucagcau aaguugggau gauagcgccg caaauaaagu aguuuguaug     1860

agauacgcaa acagggaaaa aggaaauaga acacccuuug aagcauuugg acauagacaa     1920

gggagacccu augacugggc ugauauugcu gaaagagcag cuagauugcc uaggggcaaa     1980

agauggagau uuggacccgg agcacgagcu caauucgagg aauuaggaga uuuucaagcu     2040

agacuguuaa acgaaacuag cuggcuagcc agaguugcaa aacaguacuu ggcugcagua     2100

acgcauccuc accgcauuca cguacuucca ggcagacuua cagcacuacu acgagcaaca     2160

ugggaauuga augaccuuuu gcccggauca gacgauagag cugcaaaauc acgaaaagau     2220

cauagacauc acgcaauuga ugcacuuguu gcagcacuga cagaucaagc acuucuaaga     2280

cgaauggcaa augcucacga ugauacaagg agaaaaauag aaguacuucu uccguggcca     2340

acguuucgca uagacuuaga aacaagguua aaagcuaugc uagucucaca uaaacccgac     2400

cauggauuac aggcaagauu gcacgaagau accgcauacg gcacaguaga gcacccugaa     2460

accgaagacg gcgccaaucu aguauaucga aaaaccuucg uagauauauc cgaaaaagag     2520

auagaccgaa uccgagauag gagacuaaga gacuuaguac gugcucaugu agcuggagaa     2580

agacaacaag gaaaaacacu uaaagcugca guacuauccu ucgcacagag aagagacaua     2640

gcaggucauc caaacggaau cagacaugua agauuaacaa aaaguauaaa accugauuau     2700

cuuguaccaa uuagagauaa agcaggcaga aucuacaagu cauauaacgc aggagaaaau     2760

gcauuuguag acauauuaca agcagaaucu ggaagaugga uugcaagagc aacgacaguu     2820

uuucaagcua accaagcaaa ugagagccau gacgccccag cugcccaacc aauaaugaga     2880

guauucaaag gggauauguu gaggauagac caugcuggag cugaaaaauu cgucaaaauu     2940

guaagauugu caccaucaaa caaccuauua uauuuaguag aacaucauca agcaggaguu     3000

uuccaaacac gacacgacga uccugaagau agcuuuagau ggcuuuucgc uucauuugac     3060

aaauuaagag aguggaaugc ugaauuagua agaauagaua cguuggguca gccauggagg     3120

agaaaaagag gauuagaaac aggauccgaa gaugcaacaa ggauuggaug gacccggcca     3180

aagaaauggc cuuga                                                      3195


<210>  4990
<211>  3198
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4990
augaauaaag acauaaauua cgcacuagga uuagacauag guauuacauc ugucggcugg       60

gcuguaauua acuuagaucu aaauagaaua gaagaucugg gaguaagaau uuuuaacgca      120

gcagaaaacu uaaaagacgg aucaucauua gcccuaccac gaagacuagc aagaggacga      180

agaagacuac ucagaagaaa agcguauaga gucgaaagag ugcgaaaauu gauauuaaag      240

aacaauauau uaacaaaaga ggaacuagaa ggauuauuca aaaauaaaga agugauugau      300

gugugggaag cgagaguaaa agggcuagau uacaaacuaa gucgugaaga augggccaaa      360

aucuuaauaa auuuuugcaa acguagagga uucaaaagua acaggaaaaa cgaagcuaaa      420

gauaaagaag caggccaaau acugucauca auaaguuaca acauagaaaa gaugaaagaa      480

acaaacucga gaaccauagg cgaauacaua uacaaugaag uuaaaaacag ugaagacagu      540

uaugccccac uuagaaacaa auuuggagaa uauaaaaugu guguaagccg agauguaauc      600

agagaagaaa uacauacgcu auuugaaaaa caaagagaau uaggaaacaa cuucgcguca      660

gaugaaauag aagacaaaua cuucgaaaua uucaacagcc aaagacccuu uagcaacuuu      720

gaagaccuag aaaaacuggu agguuuuugc acauucgaaa gaaagaaaca uaaacgagca      780

ccaaaacacu gcaucagcuc cgaggaguuc acguuauaug aagggauaaa uaaacuuucc      840

auaauaaaag auggagagaa aagaaaauua agugaagaag aaaggacgcu aguggucaau      900

gaagcauuua auaagaaaga aaucaaguac acaacacuuc gaaaauuauu aggguuaagu      960

gaagaagaau uauuuucaac acuaacauau aguauagaca aagauauuag caaaaccgag     1020

aacacaaagu ucguaucacu caaaggauau cacgaaauca agagagcgau agaaaaagga     1080

guaucaaaga aagcguggca agauauagag aacaauagaa aucuacuaaa ugauauugca     1140

uauguauuaa cauuaggaaa aacagaugac gaaauugaaa aacaacucag auuaagaaau     1200

gugccugaag aauuauuuga aagucuauua gauaugagcu ucaacaaauu caauaaucua     1260

aguauauacg cacuagagaa aauccuacca uucaugaaag aagguuauca guacaacgag     1320

gcuugcgaaa aagccggaua caauuuuaaa gccauauacg aagguguaag gacaaagaaa     1380

uugccaguua uagaaauaga cgaaauaguu aacccuguag uaaauagagc auuagcacaa     1440

accagaaaag uaaucaauag cgugauagac aaauauggaa guccgguaag aauaaacauc     1500

gaguuagcca gagacuuagc caaaaauuuu aaagaucgaa aacucauaga aaaagaacaa     1560

aaagaaaauc gugcgaaucu agauaaaaua agagagaauc ugagggaauu aauggacaaa     1620

gaaccaacag uugucgaagu acuaaaauau cgauuauggg aacaacaaag aggcgagugu     1680

gcauauacgc aaucacaaau accacuagag agacuauuua gucccggaua cugcgaaauc     1740

gaccauauua uaccguuuag uagaaguuuu gacgauagcu uaagcaauaa aauauuagua     1800

cucggcagcg aaaaccaaag aaagggaaac aggacaccau augaauacuu uggcgaagau     1860

agugaaagau ggaauaguuu ugagaucugg gucaaaggau cauaucuaaa uuauaaaaag     1920

aaagccaacc uacuaaagaa gaaauucuca gaagaagaac aaagggacug gaaagcaagg     1980

aaccuacaag auaccaaaua uauuugucgu uacaucagua auuucguaaa caauagacua     2040

gaguucaaag aaucggagaa caaacagaaa gucauaaccg ugaauggacg ugcaacagcg     2100

uauuuacguu ccagaugggg ccugaacaaa guuagagaag acggugauaa acaucaugca     2160

uuagaugcag caguaguagg aguaacaaca cagggaaugg uacagaaaau aaguaaguac     2220

agcaaggcac augaauugua cacaguuagg aaaaaugaug aguucgucga cguggaaacu     2280

ggcgaaugcg uagacgugga ugaauacaaa gaaaucagaa aagacaucuu accaagaccu     2340

uggagaggau uuucggaaga auugaaauua agacuaagcg auaauccgcu cgaagaauua     2400

aagaaaaguc cgauagaaac guauaacgaa gaguucauaa aagaaaaugu aaaaccuaua     2460

uuuguaagca gagucccuuu uagaaaaaua ucuggaaaau uauucaaaga aacgauauau     2520

agugaaagag cuuuuaaaga cggauauuuu guaaguaaaa agagcuuagu agaucuuaaa     2580

cgaggagacc uagacaacuu cuacaacuau gaaugugaua agaaacuaua cgacgcuauu     2640

uccgaacgca ugaaagaauu uaacuacaau ggaaagaaag cguucgaaag ccccuuccga     2700

aaaccaacaa aaaguggaaa agaaggucca guuguaagga gcguaaaaau aaaauccgca     2760

guaccauuua aagaugggau ccccaugaau gagggacugg uugcgaaaga aggaauggug     2820

cgaauagaua uauacgaaaa agaagguaaa uauuuuguag ugccgguaua cagauaccaa     2880

uuagcuaaag gaauaguacc uaaaagagcg gcaauagcua agaaagauga acuuaaaugg     2940

ccuauaaugg aucagacuua caauuuuaaa uucucaaucu acaaaaauga cuuaauagaa     3000

guuaaauaug auaagaaagg uguauauuuc gguuauuaug acggcuuuga uagaaacuca     3060

gcgagccuaa ccauagaaaa acacgauaau agcgaaagau acagaucuau aggguuaaaa     3120

guaggaguau uggaaauaaa uaaauaugaa guggaugucu uagguaacua ucacaaagua     3180

aaauuaggag gaaauuga                                                   3198


<210>  4991
<211>  3198
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4991
augaacaaag auauaaauua cgcacuaggg cuagauauag gaauaacaag uguagguugg       60

gcaguaauaa acuuagacuu aaaucgaauu gaagauuuag gugugcgaau uuucaacgca      120

gcugaaaacc cuaaagacgg gucaucacuc gcacuaccua ggagauuagc aagggguagg      180

cgaagacugu uaagaagaaa agccuacagg guagaaaggg uuagaaaacu gauagugaaa      240

aacaauauuc uuacaaaaga agaacuagaa ggccuauuca aaaauaaaga aguaauagau      300

guaugggaag caagaguaaa agggcuagac uacaaacuaa gccgugaaga augggcaaaa      360

auauuaauca acuuuugcaa aagacgagga uuuaaaucaa accguaaaaa ugagaccaaa      420

gacaaagaag cuggacaaau acuaaguuca auuucguaua auauagagaa aaugaaagaa      480

acaaauucac gaacaauagg agaauacaua uauaacgaag uuaaaaacag ugaugacucc      540

uaugccccgc uaagaaacaa auucggagaa uauaagaugu guguaucacg agauguacua      600

agagaagaaa uucauaccuu guucgaaaaa caacgagaau uagggaauaa uuuugcaucc      660

gacgaaauag aagauaaaua cuuugaaaua uuuaauuccc aaagaccauu uagcaacuuu      720

gaagauuuag aaaaguuagu aggauuuugc acuuucgaac guaaaaagca caaaagagca      780

ccgaaacacu guaucucgag cgaagaauuu acauuauacg aagggauaaa uaagcuaagc      840

aucauuaaag auggagagaa aagaaaauua agcgaagaag aaagaacccu aguaguaaau      900

gaagcauuca auaagaaaga aauuaaauau acaacacuca gaaaauuacu cggccucagc      960

gaagacgaac ucuuuucaac auugacauac ucgauagaca aagauauaag caaaacagaa     1020

aacacaaaau ucguuaguuu aaaaggauau cacgaaauaa aaagagcaau agaaaaagga     1080

guaucgaaaa aggcauggca agauaucgag aauaauagaa aucuacuaaa cgacauagca     1140

uacguucuaa cacucggaaa aacagaugau gagauagaga aacaauuaag acuccgaaac     1200

guaccagagg aacuauucga aucgcuuuua gacaugagcu ucucaaaguu uaauaacuua     1260

ucaaucuacg cacuagagaa aauauuacca uucaugaaag aaggcuauca auacaacgaa     1320

gcaugcgaaa aagcgggaua caauuuuaag gcaauuuacg aagggguucg aacuaagaaa     1380

cucccaguca uagaaauaga ugaaauagug aacccaguag uaaacagagc acuagcucag     1440

acaagaaaag uaauaaacag ugucauagac aaauauggca guccgguaag gauaaauauu     1500

gaauuagcaa gagaccuagc uaaaaacuuu aaagaccgca aacuaauaga aaaagaacaa     1560

aaagaaaaca gagcaaauuu agauaaaaua agggaaaacc uacgagaacu aauggauaaa     1620

gaaccgacag cuguagaagu auuaaaauac agauuauggg aacaacaaag aggagaaugu     1680

gcauacacac aaagucaaau cgcuuuagaa agacuauuua gcccagguua cugugaaaua     1740

gaucacauaa uaccuuuuuc ccguaguuuc gaugauaguu uaagcaacaa aaucuuagug     1800

uuaggcuccg agaaccaacg gaagggaaau aagauaccgu acgaauauuu uggagaagau     1860

agugaaagau ggaacagcuu cgaaauuugg guuaaaggaa gcuacuuaaa uuauaaaaag     1920

aaagcaaacc uucuaaagaa gaaauucacc gaagaggaac aacgcgauug gaaagcaaga     1980

aaucuacaag auacaaaaua cauauguagg uauauaagca acuucguaaa uaacagauua     2040

gaguuuaaag aaagugagaa caaacaaaaa gucauuacag uaaacgguag agcuacagcu     2100

uaccuaagaa guagaugggg auuaaauaaa guaagagagg acggagauaa acaucaugcu     2160

cuagacgcag cuguaguagg aguaacgacc caaggaaugg ugcagaaaau aucaaaauau     2220

uccaaagcuc acgaacuaua uacuguccgg aaaaaugaug aguuuguaga cgucgaaaca     2280

ggagaaugcg uugacguuga ugaauauaaa gaaaucagaa aagacauacu accuagacca     2340

uggagaggau uuucggaaga acuaaaauua agauuaucag acaauccgcu agaagaguua     2400

aagaaacuac cgauagaaac auacaaugag gaguuuauca aagaaaaugu aaaaccaaua     2460

uucgugaguc gaguaccauu uagaaaaaua agugggaaac uguucaaaga aacaaucuac     2520

agcgaaaggg cuuucaaaga uggauauuuu gucaguaaga aaagccuagu agaucucaaa     2580

agaggagacc uagauaauuu cuacaacuau gaaugcgaca agaaauuaua cgaugcaaua     2640

agcgaacgaa ugaaagaguu uaauuacaau gguaagaaag cauucgaaag ccccuuccgc     2700

aaaccaacca aaagcggaaa agaaggaccg guuguaagaa guguaaaaau caaaucugca     2760

gccccguuca aagacggaau accaaugaau gaaggauuag uagccaaaga agggauggua     2820

agaaucgaca ucuaugagaa agaagguaaa uauuuuguag uaccaguaua ucgguaucaa     2880

cuagcaaaag gaauuguacc aaaaagagca gcaauagcag ggaaaucgga auuaaaaugg     2940

cccauaaugg accaaacaua uaauuucaaa uucagcauuu uuaaaaauga ucuaauugaa     3000

auaaaauacg caaagaaaga uggauacuuu ggauauuacg auggauuuga cagacacaca     3060

gccagcuuag uuauagaaaa ccacgauaau uccgauagau aucgagguau cggcguaaaa     3120

acaggaguag uagaguucaa caaauacgaa gucgauguuc uaggcaauua ucauaaagug     3180

aacuugggag guaauuga                                                   3198


<210>  4992
<211>  3201
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4992
augucagaac gaguuguacg gagaauauua gggauagauu uagguauagc auccugugga       60

uggggaguag uagacauauc uggcgcuggu ggaggcauaa ucgcaacagg gguuagaugu      120

uuugaugcac cacugauuga caaaacaggg gaaccuaaaa gcgcaacacg acgaacagca      180

agaggacaaa gaaggauagu acguagacga agacagcgua ugaauggagu acgaagauug      240

uuaugcgaau uugguuuguu acccgaccca agacccgacg cauuaaauca agcuaugcgu      300

agaaucagua cagcaucagc agcagcgcaa guaacgccau ggacacucag agcagcugca      360

caccaacgcc uguuaaguaa ugaagaacuu gcaguaguau uaggucacau ugcuagacau      420

aggggauuca gaucgaacgc uaaaaaugaa gcgggagcaa acgcugcaga cgaaaccagu      480

aaaaugaaga aagcaaugga agcaacuaga gaaggacuag ccaaauauca ugcauuugga      540

gauaugauag caaaugaucc uaaauucgcg aaucgcaaaa gaaauagaga caaagacuau      600

ucacacacag caaaaagaag cgauuuagaa gaugaaguga gagcuauauu acgagcacaa      660

cuuagauuug guagugcagc ugcaacggaa accuuagcac aaacauuugc agacguagcu      720

uucuuccaac gaccauuaca agauucggag gauagaguag gcgauugucc auucgagcca      780

ggacaaaaac gagccgcuag acgagcacca aguuuugaau uauuucgauu uuugucgaga      840

cuagcgaauc ugaaacuagc ugugggacga aguccugaac gacgacuuac ggcagaggaa      900

auagcuuuag cagcgaaagg cuucggagaa acuaagaaaa caaucacguu uaaaucacuu      960

agggaagcau uagaucucga cccgaaugcc agauucagug guauaggaaa agacaaagaa     1020

gcaucauugg auguaguagc aagaacaggc ggcgcugcau acggaaccaa aacacuaaaa     1080

gaugcgcuug gugaugcucc guggagauca cuuagccgaa ccccugaaac guuggacaga     1140

auugcugaaa uacuaucauu ucgagaagau aucacaagua ucagaaaugg acuagaggau     1200

cuaggguuag acucauuagu aguagaagca uuaaugcaag ccgcggcaaa uggcgauuuc     1260

aaagaguuca caagagcugg acauauaucu gcgagagccg cccgaaacau aauaccaggc     1320

cuacgagaag gucuaguaua uucagaagca ugugccagag uaggauauga ucaugcggca     1380

agauuaucag ugccauuaga ucaaguagga ucucccguaa caaggaaagc auuaagcgaa     1440

gcacuuaaac aagucagggc aguagcaagg gaauauggac cgaucgauua uuuucacaua     1500

gaacuggcua gauccguagg uaaaagugcu gaagagagga aacaauugac cgacggaaua     1560

gaagcccgaa auguugaaaa agcuaaacgg cgcaaacaag cggaggaaca ucuugguaga     1620

gcaccaucgg acgaugaauu auugagauac gaacuagcaa aagaacagaa cuucaagugu     1680

auauauucag gagacgcuau agacccagcu ggaguagcag caaaugacac aagguaccaa     1740

guagaccaua uauuaccuug gucaagauuu ggagaugacu cuuacuuaaa uaaaacgcua     1800

uguacugcaa gaagcaauca aaauaagaga ggucggacuc cauuugaaug guuugaagcu     1860

gauaaaacag acguagaaug gauggaauac guagcaagag uugaaaacuu acaagaaguu     1920

aaaggucgaa agaaaagaaa cuauucuauu aaagaugcgg cagcaauaga agauaaauuc     1980

aaagccagaa accuaacaga cacacagugg gcaacaagau uauuagcaga cgaacuaagc     2040

agaauguuuc caccaagaga augcgagaga gcgauuugug guagggcaga cggaggaaac     2100

gacggauuaa ccauagucga agagagaaga guauuuacaa gaccaggugc uaucacaagc     2160

aaauuaagga gggcaugggg guuagaagga uuaaagaaac aagacggaaa acguguagaa     2220

gacgaccgac aucacgcagu ggacgcccug guauuagcgg cgacaacaga aucauuauua     2280

cagagacuaa cuguagaagu acaacgaaga gaaagggaag gacggccuga ugacauuuuc     2340

cauugcgccg aaccauggag aggguuuaga gcugauguca gacgcacagu uuacggaagu     2400

gaaacgaugc caggaauuuu uguaucaaga gcagaacgua gacgagccag aggaaaagca     2460

caugacgcaa caauaaaaca aauacgugaa auugaaggag aaagacucgu auuugaaaga     2520

aaaccugugg aaaaauuaac ugauaaggau uuagagaaaa uuccuauucc aaaacccuau     2580

ggucaaguuu cagaccccaa aagguuaaga gaugaauuag uagaaucucu acgugcaugg     2640

auagccgcgg ggaagccuaa agacagacca ccguuaucac caaaaggaga ugucaucaga     2700

aaaguaagga uacaaacgaa cgacaaagua agcguugaaa uuaauggagg aaccguagau     2760

cggggcgaca uggcgagagu cgauguguuc agaaagaaaa auaagaaagc gguaugggaa     2820

uauuacguag uuccaauaua uccacaccaa auaguugcgu uaaaugaccc accagaucga     2880

gcuguuauug cuuaugcuga agauaaagau uggaaagaaa uagauaguag cuacgaguuc     2940

cuguggucau uauucggauu aagcuacgua gaaaucagua aagcuaaugg cgaaugcauu     3000

gacggguauu ucaggggauu acaucgaggu acaggagcug cuucaguaug caaacacauu     3060

agcuuaggaa aagaugcuac aguuagcgga auaggccuaa aaacacuugc aucauuuaag     3120

aaauuuacaa uagacagacu gggaagaaaa uucgaaauac cacgggaagu aagaacaugg     3180

agaggagagg cuugcaccug a                                               3201


<210>  4993
<211>  3201
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4993
augucacacc uagucuuagg acuagauuua ggaccaaauu caauaggaug ggcguuagua       60

aaugacgacc cggaacaucc gguagaauca aaauuaguug acuuaggagu uagagucuuu      120

ccagaaggag ucgauaacuu ugacacaucu aaagaauugu cacguaauga agauaggaga      180

guggcaaggg gaaugagaag acaaauaagg cgcagagcaa gaagaagaca acaauuaaaa      240

caagcguuaa uagauucaaa acuauggccc agugacccua augaugaagu uguucuauac      300

aauacaaauc cauacgaauu gagagcgaga gcacucaaag aacggcuaga accacacgag      360

guugguaggg uauuacuaca cuugaaucaa aggagaggau uccugagcaa uagaaagaaa      420

gacagagggg acaaagaagu acaaggaaug uuagcagaaa uaaaugaaaa cgaacaacaa      480

uguaaagacg gaggguuuga cacuauaggu gcuuggcuag cugacaaaca agcaaauaua      540

gaccaugcua accgagaaga gaacgaucau gucagaaaua gacaccuagc gagaagucag      600

uacguagacg aguuugaagc uauuuggagu guucaagcaa aacaucaucc gaaacuacuc      660

acagaagaac uaaaaguugg caaauuagga aaacaaacuu acccagucaa accgauaccu      720

agauggcaug aacauagaca agggcuaucc ccacuagaau cuuuuggaau auauggucuc      780

auauuuuucc aaagaccaau guauuggccg aaaucaguag uaggacuaug ugaacuggaa      840

ccaaaagaga gaaggugcca gcgaagcgau cguagacaac aaagauucag acucuuacaa      900

gaaguaaaua aucuaaaaua cguugauccu gauacacauu uagaacaaaa auuagacgac      960

aaacaaagac gauuauuauu ggauaaacuu agccauacua aagaaaugac auuugaccaa     1020

auacgaaaag cacuagacuu cuuagaaucu guaaaauuua accucgaacg aggggaaaga     1080

aguagacuaa aagggguacc uauagaugca cuauuugccg caaaggacgu acuagggcaa     1140

gagugguaug acagaccuga acaacagaaa accgaaauag uagcaguauu acuagauaac     1200

gaacgggacg acgaucgaau aaucgcaaga gcagucgcag aguggggaau gacagccaag     1260

gaagccgaag caaugcuugg ugucgaccug ccugcagggu augcgaacuu aaguuguaaa     1320

gcuauagaaa aacucuuacc gcauauggaa agaggucuac ucuauaugga cgaugaugaa     1380

ucaaauucag cuaugcaugc agccggauac uuacgacgag accaacuacg uagaagaauu     1440

uucgauguau uacccgaucc ggcaagaaca ccaaacuguc caauaggcga uauaccaaau     1500

ccaguaguaa aaagaacccu aacugaaauu aggaaaguag uuaaugcaau aguaagagaa     1560

uauggaaaac ccgaugccau acauuuggaa cuagcaagau caguacaaau gggaaaggag     1620

aaaagaucag aauauucaaa aaugauucgg gacagagaag cagaacgcga uggaauagua     1680

gaaaaguuaa gagaaagcgg aguaagggug acaagagaca acauacucaa guaccaguua     1740

ugggaacaac aaaaucaaga auguauuuac aguggcaaac ccauuagcuu ugaaaaauua     1800

uuuggggagg gagguggugu agaaguagau cauauacuac cccgcucuag gucacuagac     1860

gacagucaag caaauaaaau agucugccua agaacggcca acgcuggaaa gggcgaucag     1920

acaccauaug aguggcucgc cgauacacag ccaaaacaau acgaacaagu gugccaaaga     1980

gcagggaugu uaaugaggag uggaagaaug ccauaugcga aauauagaag auuuauucaa     2040

agagaacuug aacucgacaa auuuauagca agacaauuag cagauacagg auauauaacu     2100

aaagcgacaa uggaauaccu aagguaccuc uuugauaaag accaugaugu uuuaggauug     2160

aagggacaac uaacagcaga gcuuagauau cacugggguc ucgaaacaau acuugaagaa     2220

cuaccagauu cuccagcaug gcaugaagcu gacaguggaa aauuaagacc aggugagaaa     2280

aacagagcug accacagaca ucaugcuauu gaugccguag uuauugcucu aacaaauaga     2340

agacgacuug uacaacuauc ugauauagua aaacguggag gagcacgggc acauggagaa     2400

aucuuagaag acccuuggcc aaauuuuaga gaaaauguag uccaagcuau aaagaaaaca     2460

aacguaucac accgaguaga aagaaaaguu gcaggaaaau uacacgaaga gacauuauac     2520

gggccaacac gaacaccagg ggaaugggua cuaagaaaac cugucauagc ucuaucugca     2580

aaugagguag aaagaauuag agaugcuacg auuagacaaa uaguagugag cgcacuagca     2640

gaugcuggua uugaugucgg gcgaggaaag aaaccagacg cuaagaaaau gaaagaagca     2700

uuaucaaaau uaaaaaugcc uaguggaguu ccaauacgaa aaguacgucu aacaaaacca     2760

gaacagacca uacaaccugu aagagccggc agaccugauc aggcuuuugu caagccuggu     2820

aguacacauc auuuaugcau uuuugaauuu acagaaaaug gaaaaacaaa acgagaugca     2880

guauucguaa caaugcuaga agcaaugaac agacuaaaac gacgagaacc aguaauacaa     2940

aggacucauc ccgaacgccc agacagccga uuuguaauga guuugagcgc aagagaaaug     3000

cuacuugcca acuggaaaga uggugaaaga uuauuaacau ucaaaacagc aaguagcacu     3060

caaggacaaa uuugguucgc ugaacacaca gaugcuagaa aaucagcaga uuauaagcaa     3120

uuuguugcga acgcaaauac ccuagaugca aagaaaguca caguugaucc auuaggcaga     3180

auccgguggg caaacgauug a                                               3201


<210>  4994
<211>  3204
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4994
auggggacua gacuaggacu cgacauuggc acaaauucga uugguuggug uuuauuuaac       60

cucgacggaa guggaaggcc uauaggaauc agagauauag gaguuaggau auuuucagau      120

ggucgggauc caagaagugg ugcuagucuu gcaguggagc gacgaguagc uagagccaug      180

cgaagaagac gugaccgaua uauuaggaga cguucugccu uauuggaagc auuaauuaac      240

acaggcuuaa ugccugcgga uguacaagaa aguaaagcau uaacaggaug ugauccauau      300

guucucagag cucaggcacu agaucgagca uuagaugcac aucuacuagg aagaguauua      360

uuucauuuaa aucaacgaag gggauuuaaa ucuaauagaa aagcagaaag aaaagcaggc      420

gaugauaaag aagggggaaa aauagcggac ggagcaaaag cacuagaucu ugcaaugcau      480

gaagcgggag caagaacaua uggugaguuc cuugccggac gagauagcag gagaguaaga      540

cuuagagcag aaggagacgg auaugauuuc uacccagaaa gaagacauuu agaagcagaa      600

uuugaugcaa uaugggacgc ccaggcugca uacaacccug cccuuuuaac ugacguagug      660

agagacagau uaagaagaau aauauucuuu caaaggccac uuaaagcacc aaagguagga      720

ggaugcaccu ucuuuaauga ggaaccacga uuaccaaaag cacacccccu cuuucaagaa      780

agaagacuau augaagaagu gaaccaacua gaaauaacaa gggcuggagc accuucaaga      840

aaacuaacau uagaucaaag agauaaaaua auuaggaaau uaaaaagugc aaaaacagcc      900

ucuuucucag gacuggcacg acuguuaaag uuagcagaug gugaaucauu uucaagagua      960

acagaaaaua gaacagggcu agcaggagau gauauauuua cgucauuuag ugauaaaaga     1020

uguuucggag acagaugggc acauuucgau ccgggaguuc aauggucuau aauagaaacc     1080

guuacagaag aagaggaucc ugucagauua cgagauuggu uaauggcuac acauggaguc     1140

acaguugaac aggcagaagc aaucgggaga guacgccucc cggaaggaca cgggagacuu     1200

ggagaaacag cgaguagacu uauacuugcu caauuaaaau caggcagcaa agauggacac     1260

ccuuuaacau auaacgaagc aguagaagcu gcucuaggua aaucgcauuc ugauuuuagg     1320

acaggagaaa uauuagaaca auuaccauau uauggugaaa uucuaucaag agaaauccca     1380

cccggaaguu uagauccuga ugaugaugau gaaauaaggu ggggaaagau aacuaaucca     1440

acaguacaua ucggccuuaa ucaacucagg agacugcuua augcuuuaau aaaagcacau     1500

ggacgcccag augaaauagu aguagaauug gcacgagagu uaaaauuguc agaaaaagac     1560

aaggcugagc auaauagaag gauaggagcg aauacccgag cugcggaagc aagaucaaag     1620

aaauuacuag aaaguggcca gccggauacu ggugcaaaca gaagccuccu aaaacugugg     1680

gaggaucuaa acccaaacaa cccuuuagac agaugcuguc cuuuuugugg aagacacaua     1740

gggcagaugg cucuauuuag uggcgaaaca gaaauugaac auauaauacc auacagcaga     1800

ucguuagaug acagcgcggc aaauaaagug auagcucacc gcaguuguaa cagagagaaa     1860

ggaaacagaa caccauggga agccuggggu cauacagaac guugggcaau aauagcagaa     1920

caagucagca ggcuccacaa aucgaaacaa uggagauuug caccagaugc uauugagaga     1980

uuuaaucaag aaggaggauu cauagcaaga caacuaagug auacacaaua uuuaagccga     2040

auggcaagga cauauuuauc uucauuauau ccggauacag cggguguaua cguaauaccg     2100

gguaggauga cggcaaugcu acgacgucug ugggguuuaa acgaauuauu gcccgaucac     2160

aauuauguug agaauccgca uaguggagca ccgaaaaaca gauuagauca ccggcaccau     2220

gcgauagaug ccgccguagu aggugucacu acaagaggau uacuaaauga aauaucaaga     2280

gccgcuggac gagccgaacg gcaagacuug gacagacucu ucaucgaucu accaaaacca     2340

ugggaaaccu ucagagaaga ucugcacgaa aguuuagcga gaguuguugg aucucacaaa     2400

gcagaucaug gccgaaaagg aguaccagca aaaggaaaag acgcaaccgc agcuagacua     2460

cauaaugaaa cagcuuacgg acucaccggg uuaaccagug agaguggucu uccaauugua     2520

guacauagag uaccauuacu aucucuaaaa ccugaugauu uaagcaaucc aauaagaaua     2580

ccugaugcag cauuacaacg ggcucucaga gaggcuaccg acgguaaaag cggaaaagau     2640

uuccaagaag ccuuggcuca auucaguaaa uccgacccug uauuuaaggg aauaaggcau     2700

guaagggugc gagaaacacu aaaaguaaua ccaauucgag augcaucugg gcaugcguuu     2760

aaagccuaua aaggagaugc aaaugcucga uaugauguau ggagaaugcc agauggaaaa     2820

uggguuucaa guuggagaga uagagauggu aaagcccaua gcggaauaau aacuauguuu     2880

gcagcacauc aagcagguca accaguacaa aggccucacc cagcugcuaa aagaguacuu     2940

aguuuaagac aaaaugaucu cguggcugua gaaccucaag gccaaccaag aaaaauuaug     3000

agaguuguua aguuuucaag uggcggacag auaacguucg cagaacauca agaggcagga     3060

ccacuaaaag caagggaugc aaccagucca gauauugauc cuuucaaaua uuuuuacucu     3120

ucugcuggug gucuacaaaa agcgagggca agacaaauac gaauagauga gcuuggucga     3180

auuuucgacc caggccccag auga                                            3204


<210>  4995
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4995
augaaauaug uucucggacu agacauaggu auagcaucau gcggaugggc gguuauaaac       60

caggaaaaac acagaauaga ggacuuaggc guucguauau ucgauaaagc agaaaaccca      120

aaagauggaa agucccuagc cacaccaaga agagaugcuc gaucaacacg aagaacucua      180

agaagaaaaa agcaccgcau gcaaagaauc aagauacucc ugguuaaaca ugggcuucug      240

ucaaaaacag aaauagauca cuuauacgag agcgcaacug aaauugaugu cugguaucua      300

agacucaaug cacuagaacg acggcuaaac ccuaaggaau ucgcuagagu auuaauacac      360

uuaaguaaac guagaggcuu uaaaagcaac aggaaagaaa caacccucuc agaaaaugga      420

caaauacuug aaaacauauc cgaaaaccua caaauaaugg aacaaaaaaa uuauagaaca      480

guuggggaaa ugauucucaa agauaagaga uucgaaaauc acaaaagaaa caaagauggc      540

acauacauag gaacagucac acgacaacaa cuacaagaag agauacaaau gauauuuaau      600

gcucaaagac uauauaaaaa caaucacgca acugaaaaau uugaaaguag uuaucucgaa      660

auuugggcau cucaacgacc cuaugccucu aaagaccaaa uugaaaaaau gauaggguac      720

uguacccucg aacccaaaga aaaacgagua cccaaagccu cauggucauu ccaauacuuc      780

gucgcacucc aaacaauaaa uaaucuaaga cuaauaagca aagauagaau agaagaacuc      840

ucauucgaag aaaaaaaacg aauaaugaac cuagcauuag aaaaauccau aguuaaauau      900

auagacauaa gaaaacuauu auccguacca aaagaguuuc aauucaacag ucucuccuau      960

ucagcagaca uagucgauac aaccguagaa aacaaaaaau guauagaacu aaaagaguau     1020

cacuccauca acaagguaua uaaacaaauc uauggacaaa augcacuaaa ccuacuaccg     1080

aaagauuaug acacaaucgc auauggacua acaaucuuca aagacgacaa agauauacaa     1140

gaauaucuac aaaauaaaua uguaaaugca aaaggaaaac ccauuucaaa ucuagcaaau     1200

aaaacuuacg acgacacauu uauacaaguc cuccuuacac ucaacuucag caaaaugggu     1260

caccucuccu ucaaagcccu caaaaauaua aucccuuucu uagaagaagg acucaguuac     1320

gacaaagcuu gcgaaaaagc aggauacaac uuuaaaggca caucuuauac gaaaaaaacg     1380

aaauaccucc ccguaauacc ccaaaacaca aacccaguag uacaucgugc gcuaucacaa     1440

acaagaaaag uuauaaaugc aauaauaaaa aaauauggau cacccaaugc aauacacauc     1500

gaaacugcac gagaacuaag uaaaaccuuu caagaaagaa aagaaauaga uagcauguau     1560

caagauaacu cgaaaaaaaa ugaacaugca auauguaaac uaaaagagcu aggaaacaua     1620

aacccaagug guaucaauau aguaaaauuu aaacucugga acgaacaaga cggaagaugu     1680

auguacacag gaaaauacau ugaaccccau agauuauucg aagagggaua uacagaaguc     1740

gaucacauau uaccauacuc uagaucccuu gacgacucau auaauaauaa aguacucaca     1800

cuagguacug aaaaccagag aaaaggcaau aaaacaccuu acgaguacau gagaaacaau     1860

ucuaucuggg acgaauuuga aacuagagua cagucaaaua aaaaaauaaa caaaaaaaaa     1920

caacaaaaau uauugcuaca gaacuucuca cacgcaagag aacaagaauu caucgaacgg     1980

aaccuuaacg auacgagaua cgcaacuaua uaccucucau cucucauuca acaacaucua     2040

auuuuuuccg aauccucgcg agaaaaaaaa guacacacag uaucagguau aauaaccagu     2100

caccuacguu caagaugggg auucaacaaa gauaggaaag aaggacacau acaccaugcc     2160

uuagaugccg uaauagucgc aguaaccucu gaccauauga uacaacgagu caccaaauau     2220

uacaaacuua aagaauuaaa cagaaauaga caagccaaaa gaaugcaguu cccagaacca     2280

ugggaagguu uuaggcuaga acuugaagca cguauaucgc caaauacaca acaauacuua     2340

aaaggcauaa gauucaaaaa cuacgccgac guaaaccuca gcgaaaucaa accaauauuc     2400

guaucacgua ugccaaagcg aaguaucaca ggggaacucc aucaagaaac uauuagaaaa     2460

uuccucggcu auaaugaaaa aggaaaaguu uuaacagcaa caaaaacuaa acuugaagac     2520

aucccauucg acgcgaacgg agacuuuccu auguuuggaa aagaaacaga ccuauacaca     2580

uacaacgcca uaaaagaacg auauuuaucc cacaaaaaag auaaaucuaa aaguuuucaa     2640

gaaccauuau auaaaccaac aaaaucugga gguauaggac cacuaaucaa aucaauaaaa     2700

auaauggaua cccgaacaau aguaaaccca guaaaucaag gaaaaggagu aguauauaau     2760

agcaaaauag cacgaaccga cguuuucaaa aaaggagaaa aauacuaucu aaucccaaua     2820

uacacaauag accuaaugaa aaacacacuu ccccagaaag caauaacagc cggaaaagga     2880

uacgaagauu ggacaacuau agauccauca uucacguuuc uauuuucacu auuucccaac     2940

gaucuaauac aaauaguccc auccaaaaau aaaacaauaa aagccagaac aacaguaagu     3000

aagaaagagg uacuacuacc aagccuuacc ggguacuuca aaggagucca cucuggaaca     3060

gcaggaauaa cagucgaaac ccacgacgga ucaauaauug caaaugucgg aagcaaacaa     3120

cuauuaucau ucgagaaaua ccagguagac guauuagggc acuauacaaa aaucaaaaaa     3180

gaaaaaagaa uuggcauggc aauauga                                         3207


<210>  4996
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4996
augaauaaag caguagcaua uaggcuagca cuagaucuag gauccacauc gcuagguugg       60

gcaaucuuua gacuuaacga agcaagagaa ccuaccgcaa uaauucgagc aggaguuaga      120

auauucucag acgggagaaa ugccaauagu gaaccacuag caguucacag aagaguggca      180

cgggcaauga gaaggagaag agacagacua cuaaaaagaa agaaaagaau gcaugaccaa      240

cuuguccaac auggcuuuuu cccagcagaa guagcagaga ggaaagaacu ugaaagacua      300

aauccauauc aacuacgagc uaaaggucua caugaagcgu ugacacccgg ugaguucgcg      360

agagcauuau uucauauaaa ucaaagaaga gguuuuaaaa gcaauagaaa aacugauagg      420

aaagacaaug auucaggagc auuaaaacaa gcuauuagcg aacucagaaa aagaauacag      480

gacagugauu gugcgacagc uggagaaugg uuuuggaaag aacgaaugca gcaaaaacca      540

gaaggaguua ggggacaaug gguuagggca agauauagaa aaacacccuc aacaacagau      600

gaagggaaga aacggauagg uuaugaccua uaugucgauc gagcaauggu ggaacaggag      660

uucgaugcuu ugugggcagc gcaagcugcu uugcaaccgg gcuuauucac agaagcagca      720

cgugcagaac uaaaagacac ucuacuauau caaagagauc uaaggcccgu aaaaccggga      780

cggugcacgc ucuuaccggc agaagaaaga gcaccacucg cauugccuuc aacacaaaga      840

uuucgaauac uacaagaagu uaauaauuua agacuacuag augaggcuuu aagagaaguu      900

ccauugaacc uagcucaaag ggaugcaguu guaucugcgc uagaaggaaa gaaagaacua      960

ucguucgcag ccauuagaaa auuauugaca uuaagcggga aauuuaauuu ggaagaugag     1020

aaaagagcag aauuaaaagg uaaugcaacg ucaguuauuu uggcacgaaa ggauuuguuu     1080

ggagacgcuu gggcuggauu ugacgcagca guacaagaug aaauuguuug gagauuagua     1140

ucacaagaaa gugaaggggc auuaauagca uggcugcagc agcacacagg aguagacggc     1200

guuugugcag aggccauagu uaauacaagg uuaccugacg guuacgguag acuaucaagg     1260

aaggcguugg aaagaauagu accagcacua caacgugaag ucuguacaua ugacaaagca     1320

guacaagcag caggguucgc acaucacuca gaucuaggau uugauuucga uuaugcugaa     1380

gaugaaguac aacaaguagg agaacacaca auagcaagua cuggagaagu ccaggcacaa     1440

uaugcguuca aacaauuacc cuacuaugga aaagcauuac aaaggcaugu ugccuuuggu     1500

agcggagaug caaaagacca ugaagaaaaa uguuauggaa aaauugcuaa cccuacagua     1560

cauauaggau uaaaccaagu uagaacagug guaaaugcac uuauuagaag auaugggcau     1620

ccuacagagg uaguaguaga auuagcaagg gaccuaaaac aaucaagaga acaaaagcag     1680

caaacccaaa gggagcaagc ugauaaucag aaaagaaaug aagauauaag aaaacgaaua     1740

gcaccaauac uggaaacauc uccagaacga guuagggaca gagacauaaa gaaauggauc     1800

cuuugggagg aacugaauaa gaaagacauu gcugauagac auugucccua uaguggggaa     1860

agaauuuccg caacuaugcu cuuaagcgaa gcaguagaaa uagaacacau cuugccauuu     1920

agcagaacgc uagacgauuc auuaaauaac agaacagucg caaugagacg agcgaacaga     1980

auuaaaggag accggacacc augggaagcc agagcagauu ucgaggcaca aggauggcga     2040

uaugaagcua uacuccaaag ggccgaagga augcccccua gaaaaagaua uagauuugca     2100

gaagaugguu accaacgaug guuagguaaa gaccggaacu uccuagcaag agcuuuaaau     2160

gacacgagcu auuuaagucg acuagcugcu aauuacuuaa gauuaguuug cccacaagga     2220

guaagaguaa uuccaggaca aaugacagau aaacuaagag gaaaauuugg ucuaaauuca     2280

guacuaggau uagaugguaa gaaaaaucga aaugaccaca gacaccacgc aguugaugcc     2340

ugcguaaucg gaguaacaga ccaagguuug augcaaagau uugcaaaugc uucgaaacag     2400

gcaagagaga auggguuaac aagauuggua caagauaugu uacuaccuug guggccauca     2460

uauuacgauc auguagaaag agccguuaga cauaucaggg uaagucaccg acccgaucac     2520

ggauuugaag gcgcuaugau gaaagguaca gcacauggaa uuagagaaga uggaauuaga     2580

gaagauggaa gaauaaaaca gagaccaaaa gcuaaaggcu cagcagacca uaaaaccauc     2640

acauuaauac caauagauga accacgacaa uuggcaagac acgguguaga ugcagaggga     2700

aaaccauuac cauauaaagg auacgcauca ggaaguaauu auugcaucga aaucacgaaa     2760

aauggcaaag guaaauggga gggucaagug auaucgacgu uugaugcaua uagaaucaaa     2820

gcugcagcgg augcugcggc aagggcaggu caaguaauau caaccguaga ggcagauagu     2880

auaguacgca aaucugguug ggagagguua aggggcgcac aaucacaaaa cggucagcca     2940

uuggucauga gauuagugau aggcgauagc guuagaaugg aagucgaugg ucgagaugaa     3000

guaaugagag uaguaaaaau gucuggaaaa gaaauggucu ucgcuccagu aagagaagcu     3060

aacguggaua aaaggaauaa uaugccagau gaacaggauc cauuuacuua uaccuauaaa     3120

cgggcagacc aacuacguaa agcuaaagcc agacaaguaa cgauaagucc aaucggugaa     3180

cuacgugauc caggguucaa aggauga                                         3207


<210>  4997
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4997
augcaauacg ugcuaggccu ugauauagga aucgcauccu gcggaugggc aguaauuaac       60

caagaaaaag aaagaauaga agaucuaggg guucgcauau ucgacaaagc cgaaaauccg      120

aaagauggaa aaucacucgc ggcaccacgu cgcgaugcca gaucaacaag aaggacauua      180

agaagaaaaa aacaccgaau guuaagaaua aaaauacucu uaguaaaaca aggacuccua      240

ucgaaaaaug aacuaaauca uuuauacgaa ucaacgacag aagucgaugu guggaauuua      300

cgucuugacg cacuagaaag gaaacuaaac cccaaagaau uuacuagagu auugauacac      360

cucgcuaaac gaaggggauu caagucaaac agaaaggaaa cuacacuauc agaaaauggg      420

caaauauuag aaucuaucuc agaaaaccaa caaauaaugg aacagcaaaa uuauagaacc      480

guaggagaaa ugaucuuaaa agacaaaaaa uucgaaaacc auaaaagaaa caaagaugga      540

accuacaucg gaaccgucac acgacaacaa cuccaagaag aaauacaaau gauauucacc      600

gcacaaagau uauacaaaaa cgacuaugca acagaagaau uugaaaucuc cuacuuagaa      660

aucugggcaa gccaaagacc auacgcuucu aaagaccaaa uagaaaaaau gaucggauau      720

ugcacacucg aaccaaaaga gaaaagagua ccaaaagccu cuuggagcuu ucaauauuuu      780

guagcgcuac agacaaucaa uaaccuacga uuaauaucaa aagaccggau agaagaauug      840

agcuuuaaag aaaaaaagca aaucaugaac cuagcacuag agaaaucaau agucaaauac      900

auugacauua gaaaacuacu aagcauacca aacgaauuuc aauuuaauag cuuaucauac      960

agcgcagaca cuguagauac gacacuugaa aacaagagau gcauagaguu aaaagaauau     1020

uauucaauaa acaagguuua uaaacaaauc uaugaaaaaa augccuuaaa ccuacuccca     1080

auagauuacg acacuauagc auacgguuua acaauauuca aagaugauaa agacauuuua     1140

gaauauuuac aaaacaaaua cgugaaugcc aaagguaaac cuauaaacaa ccuagcaaaa     1200

aaaaccuacg acuacaccuu uauacaagcg cuucugaccc uaaacuucac uaaaauggga     1260

caccuaagcu ucaaagcucu uaaaaacauc auuccauuuc uagaagaagg acucucauac     1320

gacaaagccu gugaaaaagc uggguauaau uuuaaaggua caucauauac ugaaaaaacg     1380

aaauaccuac ccguuauacc ucaaaauacc aacccuguag uacacagagc acuaucgcaa     1440

acaagaaaag ucauaaacgc aaucaucaaa aaauacggau caccaaacgc aauacauaua     1500

gaaacagccc gagaauuauc uaaaacauuc caagaaagaa aagaaaucga cucaauguau     1560

caagacaauu caaaaaaaaa cgaacacgcc auacauaaac ucaaggaauu aggcaacauu     1620

aacccauccg gaaucaauau aguuaaauuc aaacuuugga acgaacaaga cggaagaugc     1680

auguacuccg gaaaauacau agaaccacac agacuuuucg aagaaggaua uacagaaguc     1740

gaucacauuc uaccuuauag ucgcucacua gacgacucau acaauaacaa aacgcucaca     1800

uuaggaaccg aaaaccaacg aaaaggaaau aaaacaccau acgaauauau gggaaacaca     1860

agcauaugga acgacuucga aacacgagua cacagcaaca aaaaaauuaa caaaaaaaag     1920

caacaaaaac uccuacucca acacuuuuca uacgcaagag agcaagaauu uauagagaga     1980

aacuuaaacg acaccagaua cgcuacaaua uaccuaucaa gccuaaucca gcaacaucua     2040

auauuuucag aaagcucuag aaaaaaaaaa guucacacag uaagcggaau caucaccuca     2100

caucuccgau cacguugggg auuuaauaaa gaccgcaaag aagggcacau acaucaugca     2160

cucgacgcag ucauuguagc aguuaccuca gaucacauga uacaacgggu aaccaaauau     2220

uacaaacuaa aagaauuaaa ccgaaaucua caagcaaaac gaaugccauu cccugaaccc     2280

ugggaggguu ucagguuaga acucgaagcc agaauaagcc caaacacaca gcaauaccuc     2340

aaaggauucc gauucaaaaa cuacgcggau guaaaccuaa gugagauaaa gccuauauuc     2400

guaucaagaa ugccaaaacg aucaauaacc ggagaacuac accaagaaac aauccguaaa     2460

uucauaggau acaaugaaaa aggaaaagua cuuaccgcaa uuaagacaaa acucgaagac     2520

auaccauuug augcaaaugg cgacuuucca auguauggaa aagaaacaga ccuauacaca     2580

uacaacgcaa uaaaagaaag auuucuauca cacaaaaaag auaaaagaaa aucauuccag     2640

gaaccucuau acaaacccac caaaucagga ggaauuggac cacuaauaaa aucaauuaaa     2700

auuauagaca cucgcaccau agucaacccc guaaaucaag gaaaaggggu aguguauaac     2760

agcaaaauag caagaacaga cguauuuaaa aaagaugaaa aauauuacuu aauacccauu     2820

uacacaauug aucugaugaa aaacauauua ccacagaaag cuaucaccgc cggcaaagga     2880

uaugaagacu ggacaaccau cgauccgucc uucacauuuc uauucagcuu guucccaaac     2940

gaccuaauac aaaucguacc auccaaaaau aagacaauaa aagcccgaac cacagucagc     3000

aaaaaagaag uacuacuccc gagccuaacc ggauacuuua aaggaguaca cucgggaacc     3060

gcuggaauca cgguggaaac ccacgauggu ucaguaaucg cuaacguggg cucaaaacaa     3120

cuauugcuaa uugagaaaua ccaaguagau auacuuggcc auuacacaaa aauuaaagaa     3180

gaaaaacgaa uaggaauggc aauuuga                                         3207


<210>  4998
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4998
augccuaaau ccgcuaaacc acaacaagcc uauacacuag gucucgauau aggcaucgcc       60

ucaguagggg cagcccuacu aggacaacag cauauaaucg gacuacaugu ccgcacguuu      120

gauagggcug aaacugccaa agaaggagag ccucuaaaca aaauaaggcg ggacgcacgu      180

cugacaagac guagguuaca acguagagca cauagauuau uaagauugag aagauuauuu      240

aaaaggcaua accuaauacc cgauacugac accucuacau uuauaucagg acaaucucca      300

ugggaacuaa gagcccaagg ccuagacaga caauuaacca gugcagaaug gacuaccgua      360

cuauaucaua uaguaaaaca ccguggguuc cauucaaauc guaaaagcga aguaaaaacc      420

gaugaaaaag cuggccaaau guugucaggc guaaaccaaa accaagcucu acuaaaacaa      480

aguggauuac gaacgauggg ugaacuugca gcuagacacg aggcuuuccg ugaagcuaaa      540

aggaauaaga aaggugcaua uaaccauaca uuuagccgac augacuuaga agacgaacua      600

cauuuauuau uuucucgaca acgugaauua ggaaauccau acgcaacacc agaauuagaa      660

gaugccguac auacauuauu aaugcagaga cgacccgcau uaucaggaac caacuuguua      720

aaaaugguug gacacugcac auuagaacca gaugaauuua gagcaccaaa agcaucauau      780

accgcagaac gauucauaug guuaacaaaa uuaaauaacc uccgaguuag uggaucuggu      840

gaaacccguc cucuaaauga auccgaaaga caggcgcucc uaagcaaacc cuuuucacaa      900

uccaaauuga cauauaaaca gguacgaaaa auccuagaac uaccggauaa uauucgauuu      960

guaggucuac auuaucaucc cgcccaagac aauggaaaaa guccagagaa ucguguuuuc     1020

uuugaagcaa aagccuuuca ugcauugagg aacguauaua agaaagcagg auuaaaccuu     1080

gaauggcaaa gggacucaca aaacccagaa aaacuagaua auauuacaua ugcacugaca     1140

guuuuuaaag aagauaaaga agcaaaacaa ugguuaauag aaaaaggagu cgaagcauca     1200

guaauugagg caguccugaa cgagagcuuc agcgaauuug uaagacuauc uaucaaagca     1260

uuacggaaga uaauacccca uauggaaauu ggacaucgcu acgacgaagc aguaaaauua     1320

gccggguauu cacaucacag ccaucuacca caaccuucaa aaacaccuua cauaccaagg     1380

uuuucaaaac agaguauagc aaauccuguc gucucaagag ccuuaaacca agcacgcaag     1440

cuaguaaaug caauaguacg agaauauggc ccucccgaug caguacauau agaacuagca     1500

cgugaccuga acaaaccuuu cgaugaacgc aggaaaauag aaaaagauca agaaaaauac     1560

caaaaagaua aagcaagaga uaucgaaaca uuccaggaaa auuuugguuu cacaccuaaa     1620

ggacuacaac ucacuaaaug gagacuauau agagaacaau caggcaaaug ugcauacuca     1680

cuuaaaccau uagacauaaa cagauuauuu gaggacggau augucgaaau agaucaugca     1740

cuucccuauu caagaucauu uaacgauggu augaauaaua aaguccugac acucacuacu     1800

gaaaacagaa auaaaggcaa ucaaacaccg uacgaauacc uagauggugc aaguaacagu     1860

gcccaaugga gaaacuuugu agcuugggua aacucuaaca aaucuuauag ggcaucgaaa     1920

caaagaaauc uacuccgaaa agauuuugcu gcacaagccg cuacagaguu ccgagaaagg     1980

caccuaacag auacaaggua uaucgcccgg gaguucaaac guaugguaga aacucaccua     2040

aaacuuaaca aaccuaacca aagaugcgua gucgucuccg gacaacucac ggcuuauuua     2100

agagcaagau gggguuuaau uaaaguaaga gaaaacggag aucuacacca cgcauuggac     2160

gcagcaguag uagcagcaug cucacaugca augguaaaaa gauuagcaga cuauaguaga     2220

aggggcgaau ugcaauacgc ucgaggccau cacuuugauc cagaaacagg agaaauaaua     2280

gauauuaaug cauugagaaa acucgacgac cacuuuccac agccauggac ugauuuccga     2340

cgugaacuau uggcauggcu aucaccagac ccugcgcaac agcuaaagaa ucuagcguau     2400

uauacagacg aacgauuaaa acaaguaaaa ccaauaagag uaucaagagc accgacucga     2460

cgcggauuag gugcagccca ucaagaaaca auaaggagcg caaaauaucu aggagaaaac     2520

cguuccacag ucaaaacucc ccuagaaaaa cuaaaacuua aagacauacc aaauauagua     2580

ggauaugaag aucccagaaa ucaccaacuu auccaagcaa ucagccaaag auuaacagaa     2640

cacggagaug acggaaagaa ggcuuucaaa gaacccuuau acaaaccagg aaauaauaaa     2700

aguacagcac caguaguacg aucaguaaaa cucuuuacag ugcagaaaag uggaauccca     2760

guaagaggag gaauagcgaa uaacggaaac augguucgug ucgauauuuu cacaaagaaa     2820

gaaagauacu uugcuguccc aguauacgua uccgacgcag ccagaaacga auuaccaaac     2880

aaagccgucg ccagaggcaa agauggaugg auagacaucg augacagcuu uucauuccua     2940

uucucauuau acccaaauga uuggguauca gucguaguaa aaccacauga agcacccugc     3000

gaaggauacu augccggacu agacauauca acuggagcua uccauauaug ggcgcacgac     3060

cgaaaccgaa gcguagguaa aaauggacuc augcgaagca uaggaauaaa aacagcucac     3120

uccguagaaa aauaccacgu cgaucuccuu ggaaacuuau acaggguggu acacgaaacu     3180

agaaaacccc uaagagccgg aaaauga                                         3207


<210>  4999
<211>  3207
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  4999
augcaauacg uauuaggacu agauaucgga auagccucuu guggaugggc cguaauaaac       60

caagaaaaag aacgaaucga agacuuagga guaagaauau ucgauaaagc agagaauccg      120

aaagauggca aaucuauggc aaccccacga cgcgacgcaa gauccacacg aagaacauua      180

agaagaaaaa aacauagaau gcaaaggaua aaaauaaucu uagucaaaca aggcuuacua      240

ucuaaaacag aacuugacca ucuauaugaa ucugccacug aaguagacgu auggaaucua      300

agauuagaug cucuugaaag aaaauuaaac ccaaaagaau uuacgcgcgu auuaauacac      360

cuugcaaaga gaagaggauu uaaaagcaau agaaaggaaa cuacauuauc agaaaauggc      420

caaaucuuag aaagcauaag cgaaaaccaa cagauuaugg gacagaaaaa cuaucgcacc      480

guaggagaaa ugauauuaca agauaaaaaa uucgaaagcc auaaaagaaa uaaagacgga      540

acauacauag gaacaguaac aagacagcaa uuacaagaug aaauacagau gauauucaac      600

gcccaaagac ucuacaagaa caauuacgcc acagccgaau ucgaaaacuc auauuuggaa      660

auaugggcca gucaaagacc auacgcaagu aaagaccaaa uagaaaaaau gauuggguac      720

ugcacauuag aacaaaaaga gaaacgagca cccaaggcga gcuggaguuu ccaguauuuc      780

guagcuuuac aaacaauuaa caaucuaaga cuaauaagua aagaccguau agaagaacuu      840

ucauucgagg aaaaaaaaca aauaaugaac uuagcuuuag aaaagucuau cguaaaauau      900

auagacauaa ggaaauuauu aucgguccca aacgaauuuc aguucaacuc uuuaucuuau      960

ucggccgaca caguagacau aacagucgaa aguagaaaau guauugaacu aaaggaauac     1020

cacaguauaa acaaaguaua caagcaaauc uaugaaaaaa acgcgcuaaa uuugcuuccg     1080

aaagacuaug auacaauagc uuacggacua accauauuua aagaugacaa agacauacuc     1140

gaauacuugc aaaacaaaua uguaaaugcu aaagggaaac caaucucaaa cuucgcaaac     1200

aagacauacg augauacguu uauacaagca uuauuaaacc uaaauuucac aaaaaugggu     1260

cacuuaagcu ucaaagcacu caaaaauaua auaccauuuu uagaagaagg cuuaagcuau     1320

gacaaagcau gugagaaagc aggauacaac uucaaaggca caucuuacac agaaaaaaca     1380

aaauaucuac caguuauacc ccaaaacacc aaccccgucg uacaucgagc ccuaagccag     1440

acaagaaaag uaaucaaugc uauaauaaaa aaauauggaa gucccaacgc aauacacaua     1500

gaaaccgcac gcgaauugag uaagacauuc caagaacgaa aagaaauaga cucaauguau     1560

caagacaaua gcaaaaaaaa ugaacacgca auauguaaac uaaaagaacu uggaaacaua     1620

aauccuucag gaauaaauau agucaaauuu aaacuuugga acgaacaaga uggucgcugc     1680

auguacucag gaaaauacau agaaccacac agauuauucg aagaagguua caccgaagua     1740

gaccacauau uaccuuacuc gcgcucauua gacgauucuu acaauaauaa aacacuuacc     1800

cucggaacag aaaaucaaag aaaagguaac aaaaccccau acgaauacau gggaaauacc     1860

ucaauaugga acgauuucga aacgaggguc caaucaaaua aaaaaauaac caaaaaaaag     1920

caacaaaaac uacuauuaca acauuuuucc uacgccagag aacaagaguu uaucgaacga     1980

aacuugaaug acacaagaua cgcaaccauc uaucucucgu cauuaaucca gcaacacuua     2040

aucuucagug aaucuucaag aaagaaaaaa guacauacag uuucgggaau uauuaccagc     2100

cacuugcgaa guagaugggg cuuuaacaaa gacagaaaag aaggccacau ucaucacgca     2160

cuagaugcag uaauaguagc uguaacauca gaccauauga uacaacgagu aacaaaauau     2220

uauaaacuaa aagagcucaa ucgaaaucuu caggcaaaac gcaugccguu ucccgaacca     2280

ugggaaggau uccgccuaga acuugaagcu agaauauccc caaacacaca acaauaccua     2340

aagggguuca gauuuaaaaa cuacgcagac guaaaucuaa gcgaaauuaa accaaucuuc     2400

guuucaagaa ugcccaaaag aagcauaaca ggggaauuac accaagaaac gauaagaaaa     2460

uuuauaggau auaacgaaaa gggaaaagua cuaacggcua ucaaaacuaa acucgaagau     2520

auaccauucg acgcaaacgg agacuuuccc auguacggca aggaaacaga ucuguauaca     2580

uacaacgcaa uaaaagaacg cuuucucucu cacaaaaagg auaaaagaaa aucuuuucag     2640

gaaccccucu auaaaccaac caagagcgga ggcauaggac cauuaauaaa aagcaucaaa     2700

auuauugaca caagaacaau agucaacccu guaaaccaag gcaaaggggu cguauauaau     2760

uccaaaauag cccgcaccga cguauucaaa aaagaugaaa aauauuaccu aaucccgauc     2820

uauaccauag accuuaugaa aaauauccuu ccacaaaaag cuauaacagc ugguaaaggu     2880

uaugaagauu ggacaaccau agacccauca uucaccuuuc uauucaguuu auuuccaaau     2940

gaucuuauac aaauaguccc cagcaaaaac aagacgauaa aagcacgaac uacaguuuca     3000

aaaaaagaag uacuacuccc aucccuaaca ggauacuuca aagguguaca uucaggaacc     3060

gcaggaauaa ccguagagac acacgacggg agcguaaucg ccaacguugg gucaaaacaa     3120

uuacuauugu ucgaaaaaua ccaaguugac guccuaggac acuacacaaa aauaaaggaa     3180

gaaaaacgaa uaggaauggc aauauga                                         3207


<210>  5000
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5000
augaagaaac aaccgcaaag auauaucuua ggacuggauc uaggaaucgc aaguguugga       60

ugggcucuca uauuagugga cggagcagaa aacccagugg gguuauuaga uuguggcgug      120

agaacauucg aacgugcuga aguaccaaaa acaggcgacu cccuagcccu agcacgaaga      180

caggcaagaa gcguaagacg auuaauacga agacgagcac aucggcuauu gagacucaga      240

agacuauuaa aaagagaagg aauacuauca gcagcagacu ucaauucaga uggacuaccu      300

aaucugccaa uugacgcaug gcaacuaaga guagccggcc uagaaagaaa auuaacaaac      360

caagaauggg ccgcuguacu acuccaccua gucaaacaca gaggauauuu aucucaaaga      420

aaaucagaaa cucaaacuga aaauaaagaa cuaggaagau uacuagcagg aguagcugaa      480

aauucuaagc uacuuucaga aagcucugaa caauaccgca caccagcuga auuggcugua      540

aaauuauuug cagaacaauc aggcagauua cgaaacaagg gaggcgauua uacacauaca      600

uuuaacagau uagaucuaca gaaagaauug caccuauuau uugaaagaca augugcauua      660

gguaacccau ucgcauccgc agaauuacaa aguaaaguag augaacuacu aaugacacag      720

cguagugccu uacaaggaga agcaguauua aaaaugcuag gaaaaugcac auucgaaccc      780

ucagaauaca aagcagccaa aaauacuuau ucagcagaac gauuuauaug gcuuacgaaa      840

cuaaauaauc uacggauauu auacaaugga gaagacagag cacuugauga aggagagcgc      900

aaacaauuau uagaugaacc cuacaagaaa gcgaaacuua cauacaagca aguaaggucc      960

auauuaaacc ugccggaaag ugcaguauuu aaaggcuuac gauauggaaa agauucugac     1020

gguuuaaaag cugaaaaugg agcauuaaug gaaaugaaag cauaucauaa aauaagaaca     1080

guguuagaaa aagcagguuu aaaaggagaa ugggaaaaua gaauuagaaa cccagaaauc     1140

cucgaucaaa uuggaacugc cuuuucaguc uacaaaacag acgaagacau aaaagccgaa     1200

uuagcagcuc uuaggcugcc ugaaaaugua cucgaugcgc uccuagcugg ucuaaacuuu     1260

gauaaauuua uacaacuaag ucuaaaagca uuaucaaaua uauuaccuuu aauggaaaga     1320

gguauaagau augaugaagc guguaaggaa guguacggag aucaucgggc auuagccgaa     1380

ucaaagaacc aaaugcuauu accgcaaaua cccgcaaacg aaauuagaaa uccaguggua     1440

uuaagaacgc ugacacaggc cagaaaagug aucaaugcua ucauaagaag auacgguagu     1500

ccccaaagag uacauaucga aacagcccga gaauuaggaa aaucauuuaa agauagaaaa     1560

gaaauagaga aaaggcagga agaaaacaga aaagaacgag aaagagcagc cgaacauuuc     1620

aaagaacuau uccccagauu uacaggcgaa ccaaagucug gagauauacu uaaacugaga     1680

cuguaucaac aacaacaagg caaaugucug uacucaggaa uaccuauaga cuuaggaagg     1740

uuaaacgaaa agggauacgu agaaaucgac caugcacuac ccuuuaguag aacaugggac     1800

gauucauuua acaauaaggu ucuaguauua ggaucagaaa accagaaaaa gaggaaucaa     1860

acacccuaug aauaucuaga uggagcacgg gauucccaaa gauggagaga auuucaagcu     1920

aggguacaag gauguagguu uccauacgca aagaaacaac gaauacaaac cgcaaaacua     1980

gauagcaaaa cagaacaagg auuuuuagaa cgaaaccuua augauacaag guacauagcg     2040

agauuccuau gcggauucau ugaaaagagc uuacaccuac aaggugccgg aaaaaggaga     2100

guauuugcau caaacggaca aauaaccgcu cuacucagag guuuaugggg cuuaaagaaa     2160

guucgagaag acaaugauag acaucacgca cuugaugcaa uaguaguagc cugcaguaca     2220

gugaguaugc agcagaaaau uacgaaagcu augcaaagga gggagacccu agagacaauc     2280

aacacagaaa caggagaagu uaaaacacga auaccucaac cuugggauuu cuucagacaa     2340

gaaguaauga uuagaguauu cagcgaugcu ccacgagaag aacuagcaga gaaacucaac     2400

ucuagacccg aagcacuaca ugauuuuguu agcccccuau uugucagcag agcaccaaac     2460

aggaaaauga caggacaagg ucacuuagaa acagugaaau cagccaaaag acuuagugaa     2520

aauauaucag ucguaaagaa accacugaca gcucucaaau uaaaagacau agaaaauaua     2580

guaggauacc cagaucgaga accugcauua uauagagcau uacaagaaag auuacaagcc     2640

uauaaagacg auccagcaaa ggcauuugca gaaccguucu auaaaccagc aaaagacggc     2700

gggcagggcu cacucguaaa ggcagucaga auaauagaaa acacaaaaca uggccuuaaa     2760

gugagaaacg ggauuaccga caccaacgga gacaugguac gugcagaugu auaccagaaa     2820

gccgacaaga aagggaaaag acaauauuuu auagucccag uuuacgcaug gcagguagag     2880

aaaaacgaac ucccaacagu agacuguaaa ggcuacgaaa ucgaugacac uuacgaauuu     2940

uguuucucuu uauauaaaua ugacauuaua gaaauccaaa aucccgacaa uaugucaaaa     3000

gaauuaaggg ucuauuacau uaacugugac aguagugacg gccgauucug guuagcacac     3060

cacgauaaaa gugcaaaaga acaacaauuc agaauaagca cucaaaacca accuauaaua     3120

aagaaauauc aaguggaucc cuuaggaaaa gaaauaagau uauguaaagc cgagaaaaga     3180

acagaacuaa acggaaagaa gaagaaauga                                      3210


<210>  5001
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5001
augaacuacg auauuggacu acgcaucgga auuacaucuu guggauggag cauaauaaau       60

aaagauuuag aaagaauuga agacuuagga guaagagucu ucgaaaaagc cgaaaacccc      120

gauggaacag ccucagcugc accaagaaga gaagcuagaa aaucucgaag aaaacauaga      180

cgaaaaaaac auagaauaga aagaauuaaa agacuaauag ugcaacauga ccuacuaucu      240

aaaaaagaau uggauucauu auacuuaagc ccauuuaaca uagauguaug gaaucucaga      300

guugaggcac uagaaagaaa auuagacaac agagaauucg cacgcguacu aauacauuua      360

guacaaagaa gaggauucca aacuauaaga aaaucagucg aaauucaaga agaaggaaaa      420

cuacuugaaa auguaagcga aaaugaacga auuaugaaag ugaauggaua uaaaacagua      480

ggcgaaaugu uuagcaaaca cgaaaaauuu acacaccaua aaaggaauaa agacggauca      540

uacacaagug uagucacuag aucccuacua cuaaaagaga uaaagacaau uuucaacuca      600

caacgcauau uagguaacgu auuuucgacc caaaaauuag aaucagacua cuuauauaua      660

uggggaucgc aacguccaac cuuaacauac gaacaauuaa uguccauggu cggaaauugu      720

aucuacgaaa aaaaagaaaa aagagcacca aaaacaaguu gggcuuuuca auauuucuca      780

uuauuacaaa aaauaaacaa auugaaagua uccgacgaca acacacuaag aaaauuauca      840

aaagaagaaa gagacauagu aauugaauua gccuucaaaa aaaagaacgu aagcuucgua      900

gacauaagaa aggcucugaa auuaaacgac aauacgaaau ucaaccaucu aacauacucu      960

cauguaguug aaacuaaaaa aguugaaaaa gcaacauuua uagaauuaaa aggauaccau     1020

cugauaaaaa aacaauuaaa agacagaaac guagaacuaa cccaagaacu acaaccaaaa     1080

gacuacgacg ccauagccgc agcaucgaca uucuucaaaa augacauaga auucagggac     1140

uaccuaagaa aucaauaucu ggauucaaau ggaaaacgua aaaguaauau agccaacaaa     1200

guauucgaag auaaaguuau agcggcagua uuugaauuaa acuucuccaa ggugugccac     1260

cuaucauuua aaucacuaua caagcugauc ccacaucuag aaaaaggauu ggacuacaaa     1320

gaggcaauuu ugugcgccgg cuauaauuuc agagaugaaa auaaacgaga uaaaaagcua     1380

uuccuaccgg uaauccaaca agaaaccaac ccaguagugc auagagcacu cacacaagcc     1440

cgcaaaguaa uaaacgcaau aauaagagaa uauggcaguc ccaauacuau cagauucguc     1500

guugcaaacg aucuagccaa aacauacaaa cagcgaaaag agcucgaauc uacuuuaaaa     1560

aaaaauagag cagaaaauga aaaaaucaaa acaaaacugu uauccauggg aaucauaaau     1620

ccuacaggca uggacaucgu aaaauacaaa cucugguaug agcaaagcgg aauaugcuug     1680

uacaccggaa gacaaaugga acucagcucu cuuuucaaac caggauauuc aauaguaaac     1740

cacauaauuc cuuauaacag aucauuugau gacaccuacc acaacagagu ucucacacua     1800

acagaaacaa aauacgaaaa agguaacaag auacccuucg aauacuucgg agcagaagaa     1860

acuuguugga augaauauga aaaaagggua ucuacaucaa aaaaaauaca cgauaaaaaa     1920

agagaaaagc uuuuaauacg gaacuuugac aucgaauacg agaaugaaau gauauccaau     1980

aauguauucg acacucacua cauagguuca uacuuugaaa aauacauuga cgaacaccua     2040

caauucaaaa aaagcacaaa aaaacaaaaa guguucagua uacacgguaa caugguagca     2100

caccuccauu cucgaugggg uuuuaauaaa aauagucgug aaauauauuu aaaucacgca     2160

guagacgccg caauaguagc uguacuuaca gaucaacuua uaaggaacau ugccgauuac     2220

uacagacgcg auaauauacu agaacaaaaa ggaagccuaa aauuuccaga accauggaaa     2280

ucauuuagca uagagcugga agcaagaauu uccccucaag uaagagaucu cauaaaaaaa     2340

cuaaauuuag auuccuauuu agacgucgau auacugcaca uaaagccagu auuuaucagc     2400

aaaaaaccuc guagaaaggu aaauggagaa gcccacaaaa gcaccauacg uagucuaauc     2460

ggaauaaaug aaauaggaaa aacaguuaca ugcauaaaaa cuagacuaga agauauaccc     2520

uucgacgaaa acggugauuu uccaauguau ggcaaagaua cagacaaaua cacauauaca     2580

gcgaucaaag aacgcuacuu agaguuuaac aaagacaaag aaaaagcauu uuccacccca     2640

uuauacaaac uuaagagaaa uggagaaccg ggaaaccaaa ucaaaucagu aaaaauuaua     2700

gacacaagaa acauagucaa uaaaguaaau caaggcaaag gaauagccua caacucaaau     2760

aucauuagag uagacguauu cucacgagau caaaaauauu acguagugcc aauauauuug     2820

cgagacuuca uaaaagguuc cuuaccgaac aaaauuauca cugccgcgaa aggauuuaug     2880

caauggcccg aaacagacaa caguuacaca uuccuguuca guaucuuccc caacgaucuc     2940

auaaaaguag uaccuaaaga agggaaauuc auuaaagcca aaaacgagaa uaaagaaaac     3000

auuaaccuua cggaaauacu cggcuauuuc aaaggauuag acucaucaac aggagcauuc     3060

acacuggaau cacaugacgg uagcuuacua gcaagaggaa uuggaaccaa aaacuuacua     3120

uuaauagaaa aauaccaggu cgauguucua ggcagauaca auaaagucgg caaugaagaa     3180

agacaagaua uaaaaauaca acaaaacuga                                      3210


<210>  5002
<211>  3210
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5002
augaacuacg auauuggauu aagaauagga auaacaaguu guggauggag caucauaaau       60

aaagaccucg aacgaauuga agacuuagga guaagaguau uugaaaaggc agaaaaucca      120

gacggaacag caucugcagc accuagaaga gaagcaagga aaagcagacg aaaacacaga      180

agaaaaaaac aucgcauuga acggauaaaa cgauuaauag uacaacacga cuuacuuuca      240

aaaaaagagu uagacucauu auaucuaagc ccauucaaua uugacgucug gaacuuacaa      300

guugaagcgc uugaaagaaa acuugacaac cgugaauucg cuagaguacu uauacaucua      360

guccaacgca gaggauuuca aacaauacga aaaucuguag aaauacaaga agagggaaaa      420

uuauuagaaa acguaaguga aaacgaaaga auaaugaaag uaaacggaua uaaaaccguc      480

ggugaaaugu uuuccaagca cgagaaauuc acacaccaua aacgaaauaa agauggaagc      540

uauacaagcg uuguaacgcg aucacuacua cucaaagaaa uuaagaccau uuucaacuca      600

caaagaauac ucggaaacgu auuuucgaca caaaaacucg aauccgauua ucuauacaua      660

uggggaaguc aacgccccac uuuaaccuau gaacaauuaa ugucaauggu uggcaacugc      720

aucuacgaaa aaaaagaaaa acgggcccca aaaacaucau gggcauuuca auauuucuca      780

cuucuacaaa agauaaacaa acuaaaggua agcgacgaca acacacuaag aaaacuaagc      840

aaagaagaaa gagacauagu caucgaacua gcauuuaaaa aaaaaaaugu auccuucgua      900

gauauucgaa aagcacuaaa auuaaacgau aacacaaaau uuaaucaccu aacuuacagu      960

caugugguag aaaccaaaaa agucgaaaaa gcaacauuca uagaacuaaa aggauaucau     1020

cucauaaaaa aacaacucaa agaucgaaac gucgaauuaa cacaagaauu acaaccuaaa     1080

gauuaugaug caaucgcggc agcuucaaca uucuuuaaaa acgacauuga auuucgcgau     1140

uaucuucgca aucaauaucu cgacucaaau gguaaacgaa aaucaaauau agccaauaaa     1200

guuuucgaag auaaaguaau agcagcagua uucgaacuua acuucaguaa gguaugucac     1260

uuaucauuua aaagccuaua uaagcuaaua ccccaucucg aaaaaggacu cgauuacaaa     1320

gaagcaauau uaugugcugg cuacaacuuc agagaugaaa auaaaagaga caagaaacuu     1380

uuccuccccg ucauacaaca agaaacaaau ccaguuguac auagagcauu aacccaagca     1440

agaaaaguaa uaaacgcgau uauaagagaa uacggaagcc cgaauacgau aagguucgua     1500

guagcaaacg acuuagcuaa aacguauaaa caacgaaaag aauuagaaag cacccuaaaa     1560

aaaaacagag cagagaacga aaaaauaaaa acaaagcugc uaucaauggg aauaauaaau     1620

ccuacaggua uggacauagu aaaauauaaa cuaugguacg aacaaucagg aauaugcuua     1680

uacacuggga ggcaaaugga auuaucauca cuauucaaac cuggauacuc aauagucaac     1740

cacaucauac cuuauaaccg gucauucgac gauacauauc acaauagggu acuaacauua     1800

acagagacua aauaugaaaa aggcaacaaa auacccuucg aauacuuugg cgcugaagaa     1860

acaugcugga acgaauacga aaaaagagua uccacuucaa aaaaaauaca ugauaaaaaa     1920

agagaaaaau uauuaauacg caauuucgau auugaauaug aaaacgaaau gaucaguaau     1980

aauguuuucg auacucacua cauaggaagc uacuuugaaa aauauauaga ugagcacuug     2040

caauuuaaaa aaucaaccaa aaaacaaaag guauucucca uccacgguaa cauggucgcc     2100

caucuacauu cgcgaugggg auucaacaaa aauucgcgag aaaucuaccu uaaucaugcc     2160

guugaugccg caaucguagc aguauuaacu gaucagcuaa uaagaaacau ugccgauuau     2220

uacagacgag auaacauucu ugaacaaaaa gguucacuaa aauucccaga accuuggaaa     2280

uccuucucaa ucgaauuaga agccagaaua agcccacaag uccgagaucu cauaaaaaag     2340

cugaaccuag acucauauuu agauguagau auacuacaca uaaaaccggu auucauaucu     2400

aaaaaaccca gaagaaaagu aaacggggaa gcacacaaau caacgauaag aagcuuaaua     2460

gggauaaacg aaauaggcaa aacuguaacu ugcauaaaaa caagacuaga agacauaccg     2520

uuugaugaaa auggugacuu ucccauguac ggaaaagaca ccgauaagua uaccuacacu     2580

gccaucaaag aaagauaucu agaauuuaau aaagauaaag aaaaagcuuu caguacaccu     2640

cuuuauaaau uaaaaaggaa cggagaaccc gggaaccaaa uaaaaagcgu aaaaauaaua     2700

gauaccagga acauaguaaa uaaaguaaac caaggcaaag gaauugcaua caauaguaac     2760

aucauacgag uagacguauu cucuagagau caaaaauacu acguaguacc aauauacuua     2820

cgcgacuuua uuaaaggguc acuacccaau aaaauuauaa ccgcggcaaa aggauucaug     2880

caauggccag aaacagacaa cucuuauacg uucuuauuuu cuauuuuucc uaaugaccua     2940

auaaaagucg uacccaaaga agguaaauuu auaaaagcua aaaaugaaaa caaagaaaac     3000

auaaaccuca cagaaauccu uggguacuuc aaagggcucg acaguucuac aggagcuuuc     3060

acacuagaaa gccacgacgg aagccuauua gcuagaggga uaggcaccaa aaaccuuuug     3120

cuuauagaaa aauaccaagu agauguacua ggccgauaca auaaaguagg aaacgaagaa     3180

cgccaagaca uaaagauaca acaaaacuga                                      3210


<210>  5003
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5003
auggcuacca gauuaggacu agacauagga acaaauagua uaggcuggug uuuauaugau       60

ggugacucca uacgcgacau aggaguaaga auuuuucuag auggacggga gccuucaaaa      120

cuuacaguua gugggagugg uacacccuug gcagucaaaa ggagagacgc gagaggagca      180

cgccgaagaa gagacaggua uauaggaaga cgauccgcaa ugcuaagaga acucagaaaa      240

cacggcuugu uaccagccga uccaaaacag gccaaaguac uagaaacaca agacccuuau      300

gcacuacgag uuagagcgcu ugaugagaaa uuagaaccac accaaauagg aagggccuua      360

uuccaucuga aucaaagaag aggcuucaag ucaaauagaa aagcagaaag agcguccaaa      420

gccggugaag augguaaaau agcaacagcg acaaaauugu uagaugaaga aaugcaaaga      480

uuaggagcca gaacacuugg agaauuucuc guaaaacgag auagugaauu ugacggagaa      540

gcaggaaaac guguaagaau gagagcagac ucgaaggcuu augauuuuua uccugauaga      600

cgacauguuc uagaugaauu uagagcaaua uggaaugcac aaaaagucug gcaucccgaa      660

uuauugacag aacaagcaug gaaugacauc uuucguguaa uagaauauca gagagaauua      720

aaaccacaag uaguggggca uuguacauuu gcuggaauaa acgguguacc agcagacgaa      780

aaacgacuuc cuaaagcaca uccccuauuc caacagagga gacucuauga agaaguuaau      840

caauuaaaag uuguagcgcc cggagaagcu gggcguccuc uaacuagaga cgaacgagau      900

gcccuaauac uuaaauuaca agauaagaaa aguguuagcu uugagacacu uggaaaaacu      960

cuaaaauuaa aagaugguga acgauuuaac aaagaaagcg agaauagaaa agcacuagac     1020

ggagaugaag uaagagcggc gcuaucagga aagaaauuau ucggaacaag augguuccau     1080

uucucugcag aggaacaaug gaugauaaua gagaggcucc uagaagaaga agacucagaa     1140

acauuauuaa caugguuaaa aauagguuau agauuagaug augaagcagc guuagcugug     1200

gcaaaugcaa aauuaccaga agggcaugga agauuuggau uaucagcuac aaccgcucua     1260

cuuaaagaau ugaaagcaga uguguguaca uaugcagaag cugcuaaaag agcaggauuu     1320

caucacagug acuucagaac aggagaagua uuugaucaau uaccauacua cggagaaaua     1380

cuagcuagag aaauagcacc uggaaaacca gaauacggag augaaaacga gcgaagauuc     1440

ggcaaaauaa caaacccuac aguucacaua ggacuuaacc aauuaacuaa gcuaguaaau     1500

gcuauaauua gacuacaugg aaaaccagac uauauuuuug uugaauuggc aagagaauua     1560

aaauugaacg aaaaggagaa aauagcauau aauaggcaac uuaaagaaaa uacagaugcu     1620

gcucgagaua ggggagcgaa gcuagguaca augggaguua gagacacggg uagaaauaga     1680

cucauucuua gguuguggac agaauuaaau ccaggugaug cauuaaacag aagaugucca     1740

uucuguggag aacaaauauc acaacgaaug auucagaaug gagaagugga aauagaacac     1800

auaauacccu auagccaaug cuuugaugac ggaauaggua acaaaguacu cucacaugua     1860

cgcuguaauc gccaaaaagg agaccaaacu ccuugggaac gguggggaca cgacccagau     1920

agauggagua uaauacagga acaaguugca caucuacaua ggagcaaaca auggcgauuc     1980

ggcccagaug caagagauag auaugccgca gaagaagauu cauuccuacc gagacaacuc     2040

auagacacac aauauuuaac cagaauagcu gcuaaguauu uagccugucu uuauccggaa     2100

cgcggcgaaa gacacguauu ugcaguacac ggcgguauga cagcaaugcu acguagacua     2160

ugggaucuua auucaauacu acuagaccau aauuggguag aaaaccugaa cuccaacgca     2220

cccaaaaaca gacuagauca ucgacaucau gcaaucgaug cugcuguugu agguauaaug     2280

acgccuggac uaauugcuga aauugcaaaa gcagccgccc gagcugaaaa gcagaacuua     2340

gaucgacuau uugaagaacu accuaugccu ugggcacaac acccaaacga cagaagauuc     2400

agagaugaac uaagagagaa aauauuagca gcuacaguga gccacaaagc cgaccauggc     2460

agacaaggua aaccggaaag aggcagagau aucacagcag gcaaauuaca caacgauacu     2520

gcuuacgggu ucacuggcuu aacgucagac acuggacuuc caguuguagu acaccgaguu     2580

ccauuaacau cccuuacacc aaaagauaua gcagacccag auaggauacc ggauacuacu     2640

uugcgaggau cauuauggca agccacacga gaccuaucag gaaaggaauu ugaaaaagca     2700

uuacuugauu uuaguagaac ucaugcacag uucaaaggua uacguagagu gcgaguuaga     2760

gaaggcuuaa cauuaauacc aauaagaaac cacgcaggaa aaccguacaa aggauaucuc     2820

ggugauucca augcgcgaua uaauguaugg caaaugccug acggaagaug ggugcaucaa     2880

gugauaucaa cuuuugauuu acaucaacca ggauuuauag aacaaaggcc ccacccagcc     2940

gcuaagaaag ugcuuaguuu aagacaaaau gacuuaauag cuauagaaag agauggagca     3000

uuacgacaga uaaugagagu acaaaaauau ggucaaaaug gacaaauauu ucugauagaa     3060

cacaaugaag caggaaauau ggcagaaagg aacaaaaaua cagacgaucc uuuuaaauuu     3120

uacggccccg gggcaucagc auuaagaaau augaaagccc gacaaguaag gaucgaugaa     3180

cuugggcgaa uauucgaccc aggucccaga uga                                  3213


<210>  5004
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5004
auggcaaaag auuuugaaua uauauuagga cuagaucucg gagaaacaag uauagguugg       60

ucagucguac gacuaaauga aggaguccca gaacaucucg agagacuagg cguacguaua      120

uguagcgaug gaagggacgc gcagucccac gagccacugu caguugcaag acgaauagcc      180

aggggccuaa gaagacgacg ggauagguau auacauagga gaauaagauu aacugacgua      240

cuuguuaaau auggacuaau gccggaagac auaaaagaaa gaaagaaacu acaagcccua      300

aacccauauc gcuuaagguc ugaagcucuc gauagaaaac uagaacccua ugaauuaggu      360

agaguguuau uccauauaaa ccaaagaaga gguuuuaaga guaauaugaa gacagauaga      420

aaagauucag aaaguggagc uaugaaacaa gcaauacuua aaacaagagc gaagauggaa      480

gcgagcggcg cccguacucu aggagaguuu uuauacuuuu uaaacaaaga caaaacauca      540

acacaagaau acguaccugu aagaaugaga agucacagug aaaagaauaa gacgguauac      600

gaaauguauc cugauagagc gauguaugaa gacgaagucc auaaaaucuu cgaacaacaa      660

gaacuaucac cagaagccca aacagaaaua uuuaauacga uauucuuuca gagaccauug      720

aaaccaguug aacuuggauu uugugaauuu gaagauggag aaagacgagc auauacagca      780

ucaguaguuu uucaaaaauu uagagcacuu caacaaauaa aucaaauaua uguacaagac      840

gauuacgaga gaagaccauu aacuaaagaa gaaaaagaua uacuaaaaca cuuuuuauua      900

gaagauuuuu cagcauugga uaaaaaugga cuacucacau gggcuaaagu gcgaacucuc      960

cuaaaacuac agaaaaagaa auucaauuua gaaucagaaa gaagaaaagg auugaacgcg     1020

gacguaacaa guuggaaauu aucaaacgca gcauguuuug gaagggaaug guucagcuuu     1080

ucagacgcga agaaagaaga aauaguagca cugaucacag gagcagaaaa agaagaagaa     1140

cuuauagcag aauuaaaaac aaaauacgga uuaagcgaug aaagggcuaa aaauacagca     1200

aauguaaguu uaccagaugg aguugcaagu uuaagugcaa aagcaaugaa gaaacuuaua     1260

ccuuucuuag aagaaggaau guuauaugac gacgcaguga aacaggcagg auauguuuuu     1320

aguagcagcu uuaaaggugu acuacccgaa aaauacgaaa cauuuauuga accagaauca     1380

ggggaauguu ucgacgaauu gccauauuac ggacaaguuc uaagaagaua uguauuagga     1440

gggguuccgu ccgaagaaaa caaacuauuu ccagaaaaau auuauggaaa aauaaacaac     1500

ccaacaguuc auauuguucu aaaucaauua cgcaaauuaa uaaacagcuu aguagcuaaa     1560

uacggacauc cuagaaagau aguuguagaa auggcacgag auuuaaaacu aagcaagaaa     1620

caaaaagacg cuauagcaaa agaacaagcu cuuaaugaac gagaaaauaa aaggauagga     1680

gcggaacuag agagacuaga uauuagaaau aauuacgaua auagaaugaa auauaaguua     1740

ugggaagacc uuucaacuga ucccacaaaa agaucauguc ccuuuugcgg caaacaaaua     1800

agccaacaag auauauuuag uccugaguuu gaaauagagc aucuaauccc auuuucgaga     1860

ucauuuaugg acgguagaag caauaaaguu uuaucaugua ggagauguaa cagagaaaaa     1920

ggaaacaaaa caccauucga ggccuuugga aacgacucga cacgguggaa cgccaucaug     1980

gguagaguag aaucguuacc agaaaauaaa cgcuggaaau uugcaaguga ugcaaugagu     2040

agauuugaaa gagauggcga ucuacuagcu aggaugauga cagauacgca auauauggcu     2100

agaauagcaa gaagauaucu auguugugca gcaaauccaa aucaagugua cggaauacca     2160

ggccaacuca cagcaaaguu gagagaacau uggggauuaa auucaauauu gagugaucaa     2220

aaggaaaagg accguagcga ccaucgucac cacgcgauag acgccuucgu aguggcaugu     2280

acggaccgag gaacauugca aaaguauuca acggaaagga gagauuacgc aaaaccagag     2340

augccaucag aaaaagcacc ccacauacca uuugcagauu uuagacuuag cgauauaaaa     2400

agaguguuug augagauaau agucagccau aaacccgaua ggggaaaugc cagagcagca     2460

auccuaaaag gggguacagu cgcaaaauua caugaggaga cauauuacgg aaugggagga     2520

gaagguagua agaaaggaac uuggaaauua auacaaagga uuccaguaag cgaaauaaag     2580

aaagcgagug aucuugauug ucugauagac caaaaaggua cagcgaaucc ucuaagagua     2640

uuauuaguag gugcaaguga aagugaacaa gaacaaauaa uagcuagcua cuuugccaga     2700

agggggacac auaaaguaag gacauauaga gagaggaaua aggauguuau uuuaccauuc     2760

aaagauaaag auggaaaacu auaucgauac gcugucuaug guucaaaugc cuaugcugaa     2820

auuuauugcc cagaucgagg uaaaaaugca ggaaaauggc aaguagaaau uauuccaaau     2880

uacugugcac accaaaaagg auuuauucca aaauggcguc acacagacgc gcaugcaaaa     2940

uuaauaauga gauuauacag aaaugacaug gucgcauaug aaaaagaagg aaagacuguu     3000

aucgcccgag uaaagaaaau ggguggaaau ggaguaauug uguuuagaaa ucauuuaaua     3060

gcuaaagagg agggagacag acugucaugg caagcaguag uaucagggau gcaaagcgca     3120

aaccuaagaa aaauauaugu agauguccug ggccgaguaa uugacccaaa aaggccgaug     3180

acucaaaaug aaacagacgg acaggacaau uga                                  3213


<210>  5005
<211>  3213
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5005
augucacacu uuagauuaaa acaauuaaau uauauacugg gacuugaccu aggaauagca       60

ucuguugguu gggcuguugu ggaaaucgau acagaagaaa acccaacucg acuaauagau      120

guaggaguaa ggacauucga acgcgcagaa guaccuaaaa cuggcgauuc acuugcagca      180

gcuagaaggc aagcucgauc agcaagacga cuuauaagaa gaagaaccca uagguuacua      240

agggcaagaa gacugcuaaa aagagaaggu auauuacacg cugacgaucu aaaugcugaa      300

ggccuaguua aagaacuucc aaacacacca uggcaauuac gaguugacgg ccuugauaga      360

aaacuaaugc caaaagaaug ggcagccguu cuucuucacu uaguaaaaca ucgaggcuau      420

uugucacaaa ggaaaaauga aagcgaagga gccgauaaag aauuaggaag acuauuagcu      480

gguguucaug aaaaccacca agcacuacaa ucaucagaau auagaacccc ugcagaacua      540

gcaguuaaga aauuucaagc acaaagcggu cacauuagaa aucagagagg agacuauaca      600

cauacauuug acagaaagga uuuacaacaa gaauuacauc aacuauuugc agcacaacaa      660

aaauauggaa aucccuuugu cagugaugga uuaaaacaag gcaucgaaac auugcuaaug      720

caacaaagac cagcccuaag uggugacgca guacagaaaa uguuaggucg augcacauuc      780

gaaacugaag aguucaaagc agccaaacac acguauucgg ccgaaagauu uauauggcua      840

acuaaacuaa acaaucugag aauacaagaa aacgggaaug agaggugcuu aaccgcccaa      900

gaacguggcc aaaucauuga cuuaccguac aagaaagcaa aacuuacaua ugcacaagug      960

agaaaagaac uaucuuuacc agaaaacgcc uucuuuaaag gucuacgcua uggcaaagaa     1020

aaugcggaaa cagcugcuuu gauggaaaug aaagcguauc acgccauuag gaaagcauua     1080

gagaaagcug gauuaaaaac ggaauggcaa ggacuagccu gcaaaccaga acuucuagau     1140

gcaauaggua cagucuuuuc ucucuauaaa acugaugaag auauaagcac aagauuacaa     1200

ggacaacuau cagaagaugu uauuaaugca cuucucgccg gauuaaacuu ugauaaauuc     1260

auacaacuuu cuuuaaaagc cuugucaaaa auacuuccau uaauggaaca aggacaaagg     1320

uacgacgaag cccacgcagc ccuuuacgua agaucaacac cucaaccuca acgccucuua     1380

ccacagauaa cggcagauga aauaugcaau ccgguaguac uaagaacgcu aucacaagcu     1440

agaaaaguaa uaaaugcuau cguuagacga uauggaucac cagcaagaau ccauauagaa     1500

acuggccgag aagugggaaa aaguuuuaaa gauaggcaag agauacagaa aagacaagag     1560

gauaaccaca aagaacgaaa acaagcagca gaaaaauucu uagaauauuu cccuaauuuu     1620

guaggucaac caaaagccaa agacauauua aaacugagau uauaccaaca ucaacacgga     1680

caaugucuau auagcgggaa aacccucgau auaugccgac uuaaugagaa agguuacgcc     1740

gaaauagacc augcacuccc auuuaguaga acuugggaug auaguuucaa uaauaaagug     1800

uuaguuuuag cuggcgagaa ccaaaacaaa gggaaucaga ccccauauga gugguuaggc     1860

ggggcaucaa acucaccaag guggcaagag uucguagcca gaguuggcag cagcagauuc     1920

caccacagca agaaacaaag aauauuauua agacaacucg acgaagaagg cuucaaagaa     1980

cgaaaccuua acgacaccag guacguagcu cgguuuuuau guaaauucau cgaagaaaac     2040

augcucuuac uaggacgcgg aaaacgacgc guauucgcau caaauggaca aauaacaucc     2100

uuucuuagau caagaugggg ucucagaaaa gucagagaag aaaaugacag acaucaugca     2160

uuggacgcag uaguaguagc augcucaaca guagcuaucc aacaaagaau uacguacuuc     2220

guuagacaga gagaaaugaa uauauuugau ggagaauuaa uagauaaaga aacauccgaa     2280

gucaguagac cauacuuccc ucaaccaugg gacuucuuua gacaagaagu aaugaugaga     2340

guauucggca aaccagaugg agauccauca uucccggaag cagauaccaa agaaacauua     2400

uguacacuac uagaaacgag auuaucuuca agaccccaag caguacauga auaugugaca     2460

ccccuuuucg uaagcagggc gccuacccga aaaaugagcg ggcaaggaca uaaggaaacu     2520

guaaaaucug caaaguuauu acacgaagga aaaucaguac uacguguacc auuaacacaa     2580

uuaaaaccaa auuugcuaga gaaaaugguu aaucgagaca gggaaccgca auuauaugaa     2640

gcccuuaaag aaagacuagc agcauauggu aaugauccag ccaaagcauu ugcgacugaa     2700

uuuagaaaga aagguggaca auuaguaaag gcgguucgag uagaagauac ucagaaaagc     2760

ggaguauuac uacauaaagg aacaggagua gcagauaaug cgacaauggu uagaguagau     2820

guuuucgaaa aauccggaaa auauuaucuc guaccuauau acucauggca aguugcucaa     2880

gguauacucc cuaauaaagc aguaauacaa ggcaaagaug agaaaaacug ggaugaaaug     2940

gaugaaacau auacuuucaa auucucaaug uauccgaacg aucucguaga aaucaauacc     3000

ggaaaacaaa gguuauuugg auauuauaua ggacucgaua gagcgacugg agcaauaucu     3060

auuagggaac acgacuuaga caauuuuaaa gguaaaaaug gaauuuaccg uguaggagua     3120

aaaacggcca uaaguuuuaa gaaauaccaa acagaugaac uaggaaaagu uguuguucca     3180

ugccgaccga agaaaagacu accaguuaga uga                                  3213


<210>  5006
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5006
augcgacuag gccuagacau cggcacaaau ucaauaggau ggugguuaua ccaaacagac       60

gauggaacaa uaacagaugu gcuggaugga ggagugcgaa uauuuaguga uggacgugau      120

ccccaaagca aagaaucacu ggcuguagau agacgaguag cacgugcuca aagacgucga      180

cgagauaggu aucuucguag aaaagcagcg cuuaugaaaa gaauggcugg agcaggcuug      240

augccuacag aucaagcaca ugcaaaaaag uuagaaaguc ucgauccaua cgcucuuaga      300

gcggaagguu uggauuguga auuaccauua acacaucucg gacgcgcacu cuuccaccua      360

aaucaacgaa gaggauuuaa aucaaauaga aaaacggauc gaggggauaa ugagucggga      420

aaaauaaaag augcuacggc uagauuagac ugggcaauga gagaugcaaa agcaagaacu      480

uauggcgaau uccuacacac ucgucaacaa aaagcggaug acccuaaaag aaugccaacu      540

guuagaacac gcuugacugu agcgcguaga gacaaugcgg aaaaagaaga agcagguuac      600

gauuuuuauc cagauagaag acauuuauca gaagaauucg augcauugug ggcggcgcag      660

gcagauuauc augaagcacu aucagacgac cuuagagauc aaaucaaaac uauuauauuu      720

caccagcgac cuuugaagac accugaagua ggcuuaugcc uauuuacaga cgaacgaagg      780

auaccuucag cccacccauu aaaccaacga cgaauauuau uagaaacugu uaacgcccuc      840

cgaauaguag ccagaggcga agcagcaaga agacuuacau uagaagaaag ggaucaaauu      900

guucaugcau uagauaauaa aaaccauacg aaaacuuuaa gugguauggc caugaaacua      960

aaagcguuag guaaaguaau aagauuaagg ucggaucaaa cguuuacauu agaaaccgcc     1020

aauagagacg cuauagcaug ugauuuagua cgcgccagcc uaucacaucc cgacagaaug     1080

gguggaguuu ggacuaccuu agaugaagga gcacaauggg aaguaguaca acgaauaagg     1140

gcagugcaau cagacucaga auucgaugcc uugguaguau gguuagaaca aaaucacggu     1200

cuagauaaug cacaugcaca agcaacagcu aaugcaccac uaccagaagg auauggacga     1260

augggauuga uggcuacaag aagaauacua gccgcccuac aaagcgacgu aaugccauau     1320

ucugaugcag uagcagcaug cgguugguca cauaguggcg gaccuaccgg cgaaguauua     1380

gaaaaauuac cauauuaugg caaaauauua gacaggcaug uuauaccagg aacauaugau     1440

gaaaauaaug aagacguaaa acgauacgga aggauaacua acccaacagu ccacauagga     1500

cucaaucaaa uaaggcgucu aguuaauaga auaauaugca uacauggaaa accagacgaa     1560

auuguuguag aauuagcgag agauuuaaaa aauagcgaag accaaaagag agaaauacaa     1620

aaaauaauca agaagaauac agaugcagcu auaaagagag gagaaaaauu aauaaacgaa     1680

cuaggucaaa aagauaccgg agcaaacaga cuaguauuac gauuauggga agauuuagga     1740

cacgacguaa ugacaagaaa uugcccuuau aguggaaagc gaauaucggc gggcauguua     1800

uucgaugguu caugcgaugu cgaucauauu cuaccauaua gccguacuuu agacgacgga     1860

auugccaaua aaacacuaug uuugaaagag gcaaacagac aaaaaaguaa uaacacacca     1920

ugggagguau ggggagaaac agagcaaugg gacacgauag cugcaaacuu aaaaaauuug     1980

ccagauaaua aagcuuggag guuugcaucu gaugcaaugc aaagguuuga aggagaaaau     2040

gacuuuagcg ccagagcuuu aaaagacacg caauaccuaa guagaauagc aagagcuuac     2100

cuugacgcuu uauaugacgg aggagacggc aauucgcacg uauggguugu uccgggaagg     2160

cuaacugaaa ugcuaaggag acauugggga cugaacggcc uagaagcauu aacggauagc     2220

gaugaaaaca caguuaaagc aaaaaauagg auggaccaua gacaucacgc aauagaugca     2280

gcaguaguag cggcuacuga uagaggauua auucaaagaa uaucaaaaau ugcaaagcgg     2340

gaugaacaag caggugcuga ggaaguagcu agaucaguaa gcccaccuug ggacgguuuu     2400

cgagaugaua uagcagcaca acuaaguaga auaauaguaa gucaucgggc agaccacggg     2460

cgaauagaca gucaagcaaa acaacaaggu gaagaugcaa cagcaggaca acuacacaug     2520

gauaccgccu acggaguagu ggacgcggga agaguaguua guagaaagcc uuuacuauca     2580

cugagcgaau ccgauauaaa gaaaauaaga gauccagauc uacaaaggca ccuaacaaga     2640

guaacaaaag guuuagauaa aaaagaauuu gaacacgccc uaagcgauuu ugcaaacgcg     2700

agaaaauugc cagaccagag cgaaaauccu uacuuuggaa ugcgucgagu uagauugauc     2760

gaugcauuac aggauucagc acgaguauca guaagggaca gagacggcca uauauacaag     2820

gcauacaagg caggcucaaa ucauugcuac gagguaugga gacuacccga ugggaaaauc     2880

aagccauggg cuaucuccac auuugaagcu caccaaacug gaacagaaag uaagccucau     2940

ccagcggcua aacgacuauu aagaguuuuc aaacgagaca uggucgcaau agaacgugac     3000

ggggaaacuu uaaugugcua uguacaaaaa auggacauug cuaauggacu cuuucucgug     3060

ccacauacag aagguaaugc agaugcaagg aacagagaca aagaugauuc auucaaauuc     3120

auacaaauga gcgcagugag ccugauaaga gcaaagauaa gacgaauaca cguggacgaa     3180

augggaagau uaagagauca aggcccacca cgauga                               3216


<210>  5007
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5007
augagauaca cgauagggcu agacauagga auugcaagcg uaggaugggc agucauaaau       60

gaagauaaau caagaauuga agaccugggc guaagaguuu ucaaaaaggc agaagaacca      120

gacggcaaaa gucucaaccu agcuagaaga gaagcaagag ggagcagaag gagaaucaga      180

agacgagcaa caagaaugca aaaagucaaa gaacuauuag ugaaauacaa uuuaguauca      240

gaaaaagaac uagaaacacu uuacauaauc acagaaacaa caccagaugu auacgaacua      300

agagcaaaag gacuagacag acuacugagc agacaagaau uugcaagagu gcuuacuaac      360

auugccaaaa gaagagggua uaaaaguaau agaaaaauug acgaagaaga caaagaaguu      420

gguaaauuau caaaaggaac aaucgaaaac aaaaggaucu uacaagaaaa aggauacaga      480

acaauaggag aaauguuuua caaagacgaa aaauucgaau acaacaaaag aaauaaagca      540

ggagaauaua acucaaccgu acuccgauca augcuaauag acgaaguaca cacucuauuu      600

gaaaugcaaa gaaaauucaa aucagaauuu gcaaaugaag aauuugaaaa agaguaugua      660

gagguaauga cauaccaaaa accguacaua acacccgaau uacuugagaa aaugcuagga      720

aaaugcacgu ucgaaaaagg cgaacccaga gcacccaaaa acucauacac auucgaaaga      780

uucaugcuac uacaaaaagu gaauaauuug aauauauaua uaaauggcga aaaaguugaa      840

auaccacaag aaaagaagaa agaaaucauu gaacuggcgu auaaacaauc agaaauaaaa      900

uauacacaac uaagaaagaa acuagaacug cccauagagg cuagguucgc cgauuuaaau      960

uaucaaauca aaagaacaga ggacgaaagu gaggaggcuu uaguaaagaa aauugaagac     1020

aagaaauuug uaaaacucga aggcuggcac aaaauaaggu uagcauuaaa agaaaacaaa     1080

gauaaauuug agaaaauuga gaaaaauccu gagcuacaaa acauaauagc agaugcccug     1140

guaagaaaua aaaccgacga aacaauuaaa aacuaccuag aaaaaucaga cauagaugaa     1200

gaaauaauua acgcaauacu gaacaucaac uucacgaaau uuggccauuu aaguuacaaa     1260

gccaugaaua aaauaauacc agaauuggaa aaaggcuuaa cauacgacaa agccugcgaa     1320

aauguaggau acgaauuuaa aggaagaaag aacgaacuac aauauaaacu accaccggua     1380

acugaauuag aagaugaaau auuaaauagu gucguauuaa gagcagugag ccaaacacga     1440

aaagucauaa augcgauaau agacaaauac gguagcccaa cagccauuua uauagaaaca     1500

gcaagagaac uaagcaaacc auaugacgaa agaaugaaaa uugagaaaag acaaaaagaa     1560

aacgcugcaa acaacgacca aauuaaagaa uauaucaaag aaacauucgg auuugauccu     1620

aaacccuuug acauaguaaa aaugaaauua uggagggaac aaaacgguaa augcgcuuac     1680

ucaaguaaac caauaccagc agagagguua uaugaagaaa acuucgucca aguagaucac     1740

auaauaccgu uuucucgaug uuucgaugau agcuaccaaa acaaaguucu aguauugacu     1800

gaugaaaacc agaaaaagaa agaaagaaca cccuucgagu auuuugggga agacaaagaa     1860

cgguggcacc aauucgaaga guucguaaac uuaacauaua aauucaacac aagaaagaaa     1920

gaaaacuuac uaauuaaaaa uuucaaugaa gaaaaaucaa aagaauggau acaaaggaac     1980

auaaaugaca caaaauacau aucaaaauuc auguacaauu acauagccaa uaacuuaaaa     2040

uuugcagaua gugaaucaaa aagaaaaguu uauaacauaa acggacaagc cacaagcaua     2100

cuacgacacu acuggggacu aaagaaagau agagaagaau cagacaaaca ucaugcacaa     2160

gaugccguca uuauagcaug cgcaacaaac aaaaacauca agaaaguguc cgacuauuca     2220

agacgaaaau uacuguacaa aaacaaugag auaaucgacg aggaaacagg agaaauaauc     2280

gacaagaaau acaacauaga cauaagcaua aaagaaccau ggccuaaauu cagagaagaa     2340

guagaagcaa gaauggagga cgcagauaau aauggacagc ucuacgcacu gaucaacgga     2400

aacuucacaa acuaugacga uguggacaua acacauauaa agccgaucuu cacaucgaga     2460

augccugaaa gaaaaauaac cggacgugcu cacaaagaua ccaugcgauc acaaaaauuc     2520

auacaacagg gacaaaacuu uaccguggug aagaaaccac ucaaagcaau aucaaaacaa     2580

gaaaucgaag guauuauaaa caauccagac uuuaaaaauc uauacuuauc agacaagaaa     2640

uuauacgaug auauauauca gaaaaugaaa gaagcagauu uuaaagcaga aaaagcauuu     2700

gcaaaugaau acagaaagua uagcaagaac gggaacucac cuauuguaag aaguauaaaa     2760

guagcuuccg ccggugcuac aggagugcaa cuaaagaacg gagccauugc ggaaaacgca     2820

aauaugguga gaguagacgu uuuugaaaaa gauaauaaaa acuauuuagu accaauauau     2880

guuagcgauu uugccaaaaa cgaauuaccc aacaaagcaa ucugcgcaaa uaaagaugaa     2940

aaagaaugga uugaaaugac ugaaaaguau aaguuuaaau ucagucuaua uccaaacgac     3000

cugguaaaaa uaaagaggaa aaacgaaaaa gaguucuaug ccuauuacug cucaacccau     3060

agaggaacgg gaaacauaaa cuuauuauua guaaacggug aagcgaggaa agacggugua     3120

ggaauuaaaa acacagaaau auucgaaaaa uaucaagucg auguguuagg aaacauaagu     3180

aaagucaaua aggagaaaag agaaggagua aaauga                               3216


<210>  5008
<211>  3216
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5008
auggacuaua gaaucggauu agacauaggu acaaacagca uagggugguc gauaauagaa       60

cugaccaccg uucagagcaa auuagauggc aaauauagac uaaaggcagu aggccuaaua      120

gaugcaggag uaagaauguu ugacagagca gaaguuccaa aaacuggagc aucauuagca      180

cuaccgagaa gacuugcuag aagcucgaga agacgacuaa cuaggagauc ggcgagaaag      240

aaagcaauac gcaacuuauu gguaaaagaa gauauacuaa aucaaauaga auuagaucac      300

cuauaucgga cguccgagcu uguugauaua uggcaaauac gaguagaagg acucgaaaga      360

agacucggac gacaagaaug ggcaagauua uuaauucacc uuggacaaaa acgaggauau      420

aggagcacua gaaaggccga agaaggcgca gauaccgaag gaagggcagu auugaguuca      480

auaaaagaaa accaagaaac ccuaaagcaa uauagaacag uuggagaaau gugggccaca      540

gaugacaaau uuaaaggccg aguaagaaac aguacaaaug aauuuauauu uaacguauca      600

agagauaacc uagaagaaga gauaagguuu uuguuccaac aacaacgaga acucgguuca      660

auguaugcgu caaagcuauu agaagaauca uauauggaag ucuggggaca ucaacuacca      720

uuugcuuccg gcgagagcau auuaaagaau guaggaacau guagucuuua ugaaaaagaa      780

aaacgagcag cuaaagcaac auauacauuu caauauuuca uggcauuaga uaaacuaaac      840

aacguaagaa uacuuccauc aaugcggaca uuaucugaag aagaaagagg cuuagcccuc      900

aaagcaaugu uugaacgaga caaauuaccu aaaaaucccc caaaguucac uuacagccau      960

cuacgaaaag caauaaaacu agaagaagac gaaaccuuuc aaaccauauc auacgacguu     1020

gagaaaacgc uagcgacaaa cgagucagcc gucuuuguaa accuggcagc uuauuaucaa     1080

auaaagaaag uuugugauga acacaaugaa aauuaugacu auguagauua uaacacaaua     1140

ggccacgcau uaacuguaua uaaaacagac gaagacauaa auaaauacuu agcaagcaaa     1200

aacaacauug caaagcgagu auacgcuaac gacuucaucg auucaguacu caaacacuca     1260

uacaccaccu uuggacacuu aucacuaaaa gcacuaucac gaauaauacc ccuaauggaa     1320

gaaggagagg uauuugcaaa agccaaagaa aaagcagggu ucaaggaaga aagcuaccuc     1380

gaaauaaagu caaaacuucu uacuaauaua ccagacgcga uuaaaaaucc ugucguaaug     1440

cgaucuuuaa cccaggcaag aaaaguuauc aaugcaauaa uuaaaagaau gggagagcaa     1500

ccagcaagca uacaaaucga gcuugcacga gaacuaagca aggauguaag ugaacguaaa     1560

aagauaacga acggguucgc agaaaauaga aguagaaacc aauucgccau aaaaacauua     1620

gaagaaaauc acauaacaaa uauacgagga caagauauug uaaaguuuaa acuauggcaa     1680

gaacaaggag ggcgaugugc auauucacua aagccaauaa aacuagaaga acuuaucucu     1740

uuucuuaaga aagaaagaag auaugaacau aaucuagacg uagaucauau aauaccauac     1800

agcaaaacau uugacgacag cuauacuaau aaaguuuugg uuuuuagcga cgaaaaccga     1860

aagaaagggg aucaaauacc ugcugaauac cuaggaauag guacaaaaag augggaaaaa     1920

uauaaagaau uuguaauuac gucaaaauuc ccggcaagca aaaagaaacg auuguuaaaa     1980

gaacaauuag uagagagaga agaacuauuc augaaagaaa gacauuuaaa cgauacacga     2040

uacgcaagca gauauuucau gaauuuucua agagagaauc uacuuagaaa agaaacaguu     2100

cuacaugaaa cccagugggu aacagcagua gcgggaagag uaacauccca uaucaggaaa     2160

cgacuaggau uugggaaaga uagggaagaa acacaccuac accaugcuuu ggaugcuaua     2220

auagucgcgu gcaccgacaa auggaugauu gauuguguaa ccaauuauua caaauccagg     2280

gaaggaggaa guauaaaaag aaaagaaauu cgguuuccau caccauggaa cggauuuugg     2340

caugauguac aaacuaaauu agcccagcug ccacaaccug aaguuaucaa ugaauccaua     2400

ucaaaaggua acccagaugc agucgacuac augcuuguau cucguaugcc uagaaaaagu     2460

auaacuggcg cagcacacaa ggaaacuauu ucaaaauggg gaggauacga cgauaaaggg     2520

aaagcacuau uaguuaagaa aguagcacua ucagaacuaa aauuuaaaaa ugacgacuuu     2580

ccuauguaua auaaagaaac ugauccuuua uuauacgagg caauuaaaaa ucgauauguc     2640

gaaaguggaa gagacgugaa aagagcuuuu gcuacacccc uauacaaacc uagcaaaagc     2700

ggaaaacgaa augaagugaa acgaguaaaa guaauagccg aaaagaaagc uauggucaga     2760

gaagucaaug gagggauagc cgaaaauggu ggauuaguac guucagauau auuccugaaa     2820

gaaaagaaau auguaauggu acccguauau accuucgaua cgauuaaaca acaguuacca     2880

aacaaauacg uaguaagcgg ucgaggauau gaacaauggc ccgaacuuac ugcagaacac     2940

guauuccagu uuucuguaua cccauacgag cuaguacaug uaggaacaaa caaaggagac     3000

aauuccuucu auuacuucgc cucauuaaau auaagcucaa acguacuaaa auucagcgaa     3060

uguaauaggc caacaaaaga cuauauagaa auaggccuaa auucaauaau ccuguuagaa     3120

aaauacgaaa uugacguuuu aggaagaaua accggacagu caaagaaagg aauaagacaa     3180

aguuuuaaaa acaauaaaag ccaaaugaaa gaauga                               3216


<210>  5009
<211>  3219
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5009
augcgacugg gucuggacau aggaacaaau ucaguaggau ggugguuaua uaaaaccgac       60

ggggaagcug ccagcgcaag aauaguugag guuauagacg gaggcguaag aauauuuagc      120

gacggucgcg auccaaaaag uaagacaucu uuagcaguag accgaagagu agcuagggca      180

gccagaagaa gaagagaucg auaccuacgc agacgagcaa gucucaugaa acuucuagca      240

cggacgggac ucaugccugc agacccagua gaagcaaaga aacuugaaag acuagacccg      300

uaugaguuga ggaguacagg cuuggaugau gcguuaccac uaacucauuu cgggagagcu      360

uuguuucauc uuaaccaaag gagagguuuu aaaucuaaua gaaaaacaga uagaggggau      420

aaugaaaaug guaaaauacg agaggcaacu gcuagacugg accuugaaau gaugacaucg      480

ggagcaaggu cguacgguga auuucuacau augcgacgac aaagagcuuc agacccucga      540

cauguaccuu caguuaggac aagauuauca guugccgcac guggaggacc agacgcgaaa      600

gcagaggcag gauacgauuu cuacccagau agacaucauc uagaagaaga auuucaugca      660

cuuugggcag cacaagcuac acaucaucaa gcccuaacag acgacuuacg agaucuuguu      720

uuugagaaaa uauuuuauca aagaccauua aaagaaccca aaauaggguu augucuauuc      780

acagcagaag aaagaauagc aaaagcacau ccguuaacac aaagaagggu acuauucgaa      840

acuauaaauc aacuaagagu gacugcagau gggcggagua caagaccauu aacacucgag      900

gaacgugaua cauuaguaca cgcauuggau aauaagaaac caacaaaaag uaugacuuca      960

augaauauga cacugaaggc uuuagcaaaa uuguuaaaac uaagagaugg agaaagauuc     1020

acuuuagaga cagguguuag agaaaauaua ucgugcgauc cagugagagc auguauggca     1080

cacccagcaa gguuuggccc acaauggagc agcuuagauu gggaagcuca augggacguu     1140

auauccagag uucgucaagu ucaaagcgaa ggagaguuca uagaacuuac ugacugguua     1200

auacaaggac acggauuaga ugcugaucac gcuaaagcug uaggaaaugc accauuacca     1260

gaaggcuacg cuagacuagg auucucugca acagaacgca uuuuagauca auuaaaagca     1320

gaaguuugca cauauuccga cgcaguagca aaauguggau ggcaucauuc agaucaacgc     1380

acaggggcgu gucuagaagu auuaccauac uauggagaag uguuagaucg ccauguaauu     1440

cccgguacuu augaugcuac agaugacgaa auaacuaggu augggcgaau aacaaaccca     1500

acagugcaua uaggucugaa ucaauuacga agauugguca acaaaaucau agaaguacau     1560

ggaaaaccag accaaauugu aguagaauua gcacgagaac ucaaacaauc agagccacaa     1620

aagaaagaug cuaugaaaag guuaagaaac acuacugcag augcaauuag aaggucggaa     1680

caacuagaac aacuaggcca accaaacacu ggacauaaua ggaugcuccu aagauuaugg     1740

gaagauuugg gaccagcaau aggaccaaga uguugcccgu auaccggaaa aacaauaagc     1800

guaacuaugg uauuugacgg aaguugugau guugaccaua uauuaccuuu uucucgaacu     1860

uuagaugacu cauuugcaaa ucgaacgcua ugucuuaaag aagcaaauag agagaaaucg     1920

gauagaacac cuugggaggc auggggugga acugaucaau gggacgauau ugccgcuaau     1980

cuaaagaauc uacccgaaaa gaaaaggaaa agauucggac ccgacgcaau ggagugguuu     2040

gaaggagaaa acgauuuucu agacagagcu uuaguugaua cccaauauuu agcuaaaaua     2100

ucaagaaugu accuagauac acuuuuuaca aaaggagguc augucugggu ugugccaggg     2160

aggaugacag aaauguuaag aaggcauugg ggguuaaacg cacuauugaa ugauaaagac     2220

cgaggagcag ucaaagccaa aaacagaaca gaucauagac aucaugcaau agaugcagca     2280

gucguagcag caacggacua ccgacucaug caaaagauaa guagagcagc cggacaagga     2340

gaaaaugcag gacaaagcgc agaauuaauc gcgcgagaua cggacgcucc augggaacac     2400

uuuagggcag acauugcauc acaacuugag aaaauaaucg uaagccacag ggcugaccau     2460

ggaagaauag acguagaagg gcgaaaaacg ggaagagauu cuacagcugg gagauuacac     2520

aaugauacag cauauggugu uauugacgac uuaacaguag cuucaagaac accgcuuaug     2580

agucucaaac caagugauau agcaaucaca gaaagaggga aaaauauaag agauccgcaa     2640

uuacaaaaug ccuuauaugu ugcuacuaaa gggaaagaag gaaaaauguu ugaacaagcc     2700

uuaguggauu uugcuagcaa agaagggcca uaccaaggga uuagacgagu gaggcuagua     2760

gaaacacuac aaaaggcugc uagaguggaa auaggaaaga ccgaaucagg ucaaccauug     2820

aaagcauaua agggagauuc caaucacugu uaugaacuau ggaaacuccc ggauggcaaa     2880

auaguuccac aaguggugac aacauaugaa gcacaugcag gcucagcucc aagaccucau     2940

ccagcagcua aacgaauacu ucgaguauuc aagaaagaua ugguaaaacu uacgaaagau     3000

ggcgaagaaa uaguaggaua cguacaauca augcauauua aaaacggccu auuuguagcu     3060

caacaccuug aagcaaaugc ugacgcaaga aauagagaua agacagaucc cuuuaaguuu     3120

cuccaaaugg gggcaggaac auugguaaaa acaggacuua gaagaguccu aguagaugaa     3180

auaggaagac ugagagaucc ggguccaccc aagcuauga                            3219


<210>  5010
<211>  3225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5010
augcaaacua aaaauuuacc auacacacug gggcuugaca uaggaauggc aagcguaggu       60

gcugcauuac uccuacccga ccaagaaagg auauuagccc uacauguaag gacauuugau      120

aaagcagaaa cggcaaagga aggagaaagu uuaaauaaaa uaagaaggga uagucgucuu      180

acacgaagac gaauaaggag acgagcacau aggcuaguac gauuagcuag auuaaugaaa      240

agaguuggac uaauagcgga cgcaaauccu gaggcauuug cgcucccagg ugucagucca      300

uggcauuuac gagcagacgg acuagauaga cuacuagaac ccaaagaaug ggcaggaguu      360

uuguaccacu uagucaaaca cagaggauuc caaucuaacc gaaaaaguga agcaaaggca      420

gacgaaaaag cgggacaaau guuaagcgga guugguagaa aucaagccuu auuaaaagaa      480

gcaggauaua gaacagucgg agaauuggca gcaagacacg aagauuucaa agaggcaaaa      540

agaaacaaag gagguaguua uagccacaca uucgcaagag cagacuuaaa ugcagaguua      600

aaucuuuuau ucgaaagaca gagagcacuc gggucuagau uggcgagugc agauuuucaa      660

gaaaauguua gagaucuauu aauggcuaga agaccaacgu uaaguggggc aaauuugcuu      720

aaaaugguag gaaaaugcac guuugaaaaa uccgaagacc gagcaccaaa agcgggauac      780

agagcagaaa gauuuguaug gcuaggaaaa uuaaauaauu uaaaaauugu gggcagugga      840

gacgcacguc cacugacuga cgcagaacac agaauuguca uagacuuacc guuuacucag      900

gcaaaauuga cauuuaaaca aguaagaaaa gcauuagagc uagaaacgca ccaaagguuu      960

aaccuucuga guuacagacc agacccuaaa gguaaagaca aagauccuga agagucaaca     1020

uucuuugaag caaagagcuu ucauacacuc aggaaagcgu acgaaggugc aggccugaaa     1080

aaggaauggc aaagagacgc cuuagauccc gauagguuag augcguuagc auacgcauua     1140

accuguuaca aagaugacac ugaaagcaga caaugguuag uagcacaagg uauaggcacg     1200

ccaauaauag aagccguauu agcagaaucu uuugacaaau uuauucauuu aagucaaaaa     1260

gcucuaaaag cuauauuacc gcacauggaa gcaggccaac guuaugauga ugcugcaaaa     1320

ucagcuggcu acgaucacuc gcaaccagac gcaggaaccg guaaacgggc aaccuugccg     1380

ccuccagaua aagacaccau acgaaauccg gucguauauc gagcuuuaaa ucaagcaaga     1440

aagcuaguca acgcaaucgu agcagaauau ggaccaccag cagcagucca cauagaacug     1500

gccagagauu uaucaaaacc aauagacgaa cggcgagaca uagagaaagg ucaaaaagaa     1560

uuucaagcuc uaaaagaaaa acucgcagca gcuuuugaag aaacuuuuca ccgaaaaccc     1620

ucaggccugg auuuauugaa auauagacua uuuagagaac aauuagauca auguccauac     1680

ugucaaaaag cauuggucgu cgacagauua uucgaaccug guuaugcaca aauugaucau     1740

gcauuaccau acucuagauc auucgaugac ggcaugaaua acaaaguagu uguacacgua     1800

gcgugcaauc aagauaaagg aaauagaacc ccuuaugaau uucuagacgg ugcaggagac     1860

ucagaaagau ggcaacguuu uguagccugg guucaaggaa acaaagcaau acgacaagca     1920

aaaagaaacc gauuauuaag agucaauuuc gguaccgaug aggcaaaaga guucagcaaa     1980

agaaaccucu cggauacacg auauauuugu agagaauuua aaagaacuau agaaacacaa     2040

uuugcguggc auucagacgc agaaggaaau gagaggugug uaguacugag ugggcaacuu     2100

acgagcuuac uaagagcuag auggggccuc auaaaaguaa gagaagaugg ugauuuacau     2160

caugcauuag auguagcagu cauagcugcg gcgucucgau cauuaguaaa gaggauggcu     2220

gacuacucaa gaaggaauga acuagaaaug gugcgaaaua gauauguaga uccugcaaca     2280

ggugaaguag uagacguagc agcacugaga caaauagaag accacuuucc caaaccaugg     2340

ccuuuauuca gagaagaauu acuagcgcgg cuggcaccag aaccaaaauu acaacugacu     2400

uguguaagca guuaugauga agguuugaga gagaguauaa gaccuauaag gguaaguaga     2460

gcgccaacac ggagaggcuu aggagcagca caucaagaaa caauaagaag ccuuagagau     2520

gguucacaau cugcaauuaa aacucccuua agagaccuua auuuaaagga ucuggaaaau     2580

auaguuggau uugagagaga cacagcacua auagaugcga uuagaacuag auuggaagca     2640

uuuggcggag aagguaagaa agccuuugau gacaaacagc cuccacuaag aaaaccaagu     2700

aaagauccgg acaaagcucc cauaauaaga ucaguaaaac uccuagugac acaaaaaucu     2760

ggaauaccaa ucagaggagg cauugcuaau aauggagcaa uguuaagaag ugauaucuuu     2820

acaaaagcgg guaaauuuua ugcugugcca auauacguag ccgaugcugu aaggggagaa     2880

cuaccacaua gagcagucgc gcaagcuaaa ccagaacuag aauggcccau gauggaugaa     2940

aguuaucagu ucuuauucuc auuacaucca aaugacugga ugacuauccg auuaaagaau     3000

gaggucagag agggauacua cgcaggucua gauagagcga cgggaaacau aagccuaugg     3060

cuacacgaca gaaaccagaa uguuggucaa aaaggacuac uaacaggaaa uggaauaaaa     3120

accgcacuag cagucgaaaa auaccacguu gauuugcuag gcaaacuaca ucgaguccau     3180

aaagaggaaa gacagccuau acacauaaaa ccaggcaaaa gguga                     3225


<210>  5011
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5011
augaaauauu caaucggacu ggauauuggg auagcaagcg ucgggugggc uguaauaaac       60

gaagacaucg aaagaauuga agaucuuggu guuagaauau uugacgucgc cgaacagcca      120

aaaacaggug cuucccucuc aaccccaaga agauuagcua gaaguacaag aaaacgacua      180

agaagaagaa gacaccgagu agaaagagug agaaaauuca ugauacagaa aggccuacuu      240

acccaaguac agauggaaca auuauaugau uggaaagaug gagaccuaga cauauggcua      300

guaaggguaa augccuuaga aagaaaauug ucagauagag aauucgcacg cauacuugua      360

cacuucgcaa aaaacagagg auuccaaagu aaucguaaau cagaacugca agaagaagac      420

aauggagcug uauugagcgc cguacaagaa aauaaaagac ucauggaaga aaaaggauac      480

cagaccauau ccgaaauguu acuauaugac gaaaaauuug aaggaacaaa aagaaacaaa      540

ggaggagaau acacacaugu aguugcaaga agcgauuuaa aaagagaaau acaauuaaua      600

uucgaaaaac agcgagcgua cggacacgca uuugcaacaa uggaaaauga agauaaauuc      660

cuucaaauau ggucaagcca aagaccguuc gccacuaaag acgauauagc aaaaaaaaua      720

ggaaauugua cauucgaacc aaaagaaaaa agagcaccca agaguacaua cacuuuugaa      780

agauuucgug cacuggacaa acuaaauaga cuaagaauac uaucagguac aucaccggca      840

cguucauuga cacaagaaga aagggauuug auacucaauu cucuuuucag caaaaaagaa      900

guaaaauaua aagaacuaag aaaauuauua aaauugaacg acgagcaaag auuuaaugaa      960

auauucuaug accccaacga aaaaauagaa aaaaaugaaa acagaacguu ccuaucauua     1020

gaaucccaau auaaaauacg caaaauucua caaaaaauag aaggcaaauc auugaacgac     1080

acauaccguc cagucgacuu agauacgauc gcuuacgcac uaacaguauu caaagaugac     1140

caagacauca gagauuauuu acaaaaccaa uauguagcuu cuaacggcaa aaaagaauua     1200

aauuuagcaa aucgaaagua uaaugacgaa cuuaucgaag aacuacuuca uuuaucauuu     1260

acuaaauuug gacaccuauc acuaaaagcc uuaagaaaca uacucccauu uauggaacaa     1320

ggaauaccuu aucaccaagc gugugaaaaa gcuggcuacc aauucaauaa aaaaguaaac     1380

agagaaaaac agaaacuauu accucccgua ccagccaaag auauagucaa uccaguaguu     1440

auuagaaguu uaucacaagu aagaaaagua auuaauggua ucauuaaaag auauggauca     1500

cccagcagca uuuacauaga acuugcccgc gaaaugggac gagguuacaa agaacgaaaa     1560

gaccuagaaa aacaauauaa uaaaaauaga caaaugaaug aaggagcaaa aaaacacauc     1620

uuggaacuua gaccugaauu uggagacccu agaggccacg auauacuuaa auacaagcua     1680

uggcaagaac aagacugccg augcgcuuac ucauucaaau cuauaccgau uaaacaguua     1740

uucgaaauag guuacgcuga agucgaucau aucauacccu acucuagaag uuuugacgac     1800

aguaauaaua acaaagucuu aguacuggca gccgagaauc aaaacaaaca aaaccgaaca     1860

ccuuaugaau gguuugguca cgaugaaaca aaauggcagg aauuuaccuc acuuguaaca     1920

acaaugaagg uaagcaaaaa gaaaaagucu uuauuauuaa agaaaaauuu ugacgaagag     1980

caagcagaag aauucagaag uagacaccuu aacgauacac gauauauaac aagauacauc     2040

aaaacuuuua uagaggacaa ccuacaauuc cguauagaag aggguaaaaa aacauaugua     2100

cacacaguua acggugcgua cacaucgcug augaggaaac gcuggggauu uaauaagaac     2160

agacaagaaa augcuuuaca ucaugcauug gacgccguca uaguagcagu aucauugcca     2220

uuuaaacaca agguaagagc auacuuuaaa agaagagaag aauacgcuca caaccagcua     2280

aaaagagaag gagaguuuuu uccagaaccu uggccagguu uuauacacga acuugaaauc     2340

agaaugaugc aagacucaga aaacaucaaa cuagcaaugg aaucuuugaa cuuaaaaaca     2400

uacgaugaaa guuucauaaa agagcugaaa ccaaucuuug ucaguagaau gccaaaaagg     2460

gcaaugaaag gacagauaca ugaagaaaca cuaaaacgag uacggggacg gacggacgau     2520

ggauacauaa gaaccgugaa gaaagucaaa cuggaccaaa uaccuuucga caaaaacgga     2580

gacuucccca uguucgggaa agagacagac augaaaacau acgaggcaau aaaaagucgg     2640

uaucuagaau caggaaaaga caaaaaaaaa gccuucaaua caccucucua caaaccagca     2700

aaaaaucccg aaaaugcgcc uauaauaaga uccauaaaaa uagaagagaa agcaaaucga     2760

guggugaugc uagacgaaaa aaccgcagca gacaacgcaa guauaguucg aacagaagua     2820

uuuagacaca aagagacagg aaacuacuuc cuuaccccug uguauguagc agauguacua     2880

gcaaaaaaag ucccagaucg ucuaaucaca acgaaaaaac ccuauaguga auggcgcaaa     2940

auaacagacg aauaugaauu cuuauucagc cuuuacccca acgaccuuau cagaguaaaa     3000

augccaaaag aaaaagaaac aaagacucac acaggagaaa aacauguaug gcaagaaaau     3060

uuuauguacu ucaaaggagu acauagcgga agugcaggag uaagaaugau ugaccauaug     3120

aacucauuug aagaugaaau uggaguacgc aaauuagucg uauucgaaaa auaccaagua     3180

gacccgcuag ggcgaaugaa caaaguacau ggcgagaaac gacauggaau cuga           3234


<210>  5012
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5012
augaaauaua guauaggacu ggacauaggc aucgccagug uugguugggc aguaauaaau       60

gaagacaucg aaagaauaga agacuuagga guaagaauuu ucgacgucgc ggaacaaccc      120

aaaacugggg caucacuaag uacaccaaga agauuagcaa gaucaacacg aaaaagacua      180

cgaaggagac gacaucgugu agaaagagua aggaaauuua ugauccaaaa aggccuucua      240

acacaaguuc agauggaaca auuauaugac uggaaagaug gagacuuaga cauuuggcug      300

guaagaguga acgcuuuaga aagaaaauua agcgaccgag aguucgcgcg aauacucguc      360

cauuuugcua aaaauagagg uuuccaauca aauagaaaau cagaauuaca agaagaagau      420

aauggagcag uauuaucugc cgugcaagaa aacaaaagau uaauggaaga aaaaggauac      480

caaacgauau cagaaauguu acuauaugau gagaaauuug aaggaacaaa acgaaacaaa      540

ggaggagagu auacacaugu uguugcgagg ucagaccuaa aaagagagau ucaacuaaua      600

uucgaaaaac agcgugcaua ugggcacgcc uuugccacua uggaaaacga agacaaauuc      660

cuccaaauuu ggucaagcca aagaccauuu gcgacaaaag augacauagc uaagaaaaua      720

ggaaauugca cauucgaacc aaaagaaaaa cgcgcaccca aaucaacgua cacuuucgaa      780

agauucagag cacuagauaa acuuaauaga uuaaggauuc uaagcggaac cucucccgca      840

agaucauuaa cucaagaaga aagagaucuu auuuuaaaua gccuuuuuag caagaaagaa      900

gucaaauaca aagaacuaag aaaauuacug aaauuaaacg acgaacaaag auucaacgaa      960

auuuucuacg accccaacga gaaaauagag aaaaacgaaa acagaacauu uuuaucgcuu     1020

gaaucccaau auaaaauuag aaaaauacua caaaagaucg aaggaaaauc acuaaaugau     1080

acuuaucgac ccgucgaccu agacacaaua gcauacgcuu uaacuguauu caaagaugac     1140

caagacauca gggauuacuu gcaaaaucag uacguagccu caaauggaaa gaaagaacug     1200

aacuuagcaa acaggaaaua uaacgaugaa uuaauagaag aacuacuaca uuuaucauuu     1260

acaaaauuug gacaccuauc acugaaagca uugagaaaca uacuuccauu cauggaacaa     1320

gguauuccau aucaccaagc augugaaaaa gccggauacc aauuuaacaa gaaaguuaau     1380

cgagagaaac aaaaacuucu ucccccggug ccagcuaaag acauagucaa cccagucgua     1440

auuagaucgu uaagccaggu aaggaaagua auaaacggaa uaauaaaacg auacggaagu     1500

cccaguagua ucuacauaga acucgcacgc gaaaugggaa gaggauacaa agaaagaaaa     1560

gacuuggaga aacaguacaa uaaaaaccgu caaaugaacg aaggugcaaa gaaacauaua     1620

cucgaauuac gccccgaguu uggggaucca agaggccacg auaucuuaaa auacaaauua     1680

uggcaagaac aagauugcag augugcauau aguuuuaaaa guauaccaau aaaacaauua     1740

uucgagauag gauaugcaga aguugaucau auaaucccau acagccggag uuucgacgac     1800

aguaauaaua auaaaguccu aguacuagcu gcggaaaauc aaaacaaaca aaacagaaca     1860

ccuuaugaau gguuuggaca cgaugaaacu aaauggcaag aauuuacaag ucuaguaacu     1920

accaugaaag uauccaaaaa gaagaaaucc uuacuguuaa agaaaaauuu ugaugaagaa     1980

caagcagaag aguucagguc aagacaccua aacgauacga gguauauaac acguuacaua     2040

aaaacauuua uagaagacaa ccuacaauuc agaauggagg aaggaaagaa acaauaugua     2100

cacacaguaa acggcgcaua uacaucauua augagaaaaa gguggggauu caauaaaaau     2160

cgacaagaaa acgacuuaca ucaugcacua gacgccguaa uaguagcagu aagccuacca     2220

uucaaacaua aaguaucagc auacuucaaa cguagagaag aauacgcaca uaaucaacug     2280

aaaagggaag gggaguucuu uccugaaccu uggccaggcu uuauacacga auuagaaaua     2340

agaaugaugc aagacucaga aaacauaaaa uuagcuaugg aaaguuuaaa ccuaaaaacu     2400

uacgaugaau cauucauaaa agaacuuaaa ccaaucuucg uaucgaggau gccgaaacga     2460

gcgaugcgag gucaaauaca cgaagaaaca uuaaaaaggg ucagaggaag aacugacgac     2520

ggauauauca gaacagucaa gaaaguuaaa uuagaccaaa ucccauucga uaaaaaugga     2580

gacuucccua uguucggaaa agaaacugau augaaaaccu acgaagccau aaaaaguaga     2640

uacuuagaaa guggaaaaga caaaaagaaa gcauucaaca ccccgcuaua uaaaccagcc     2700

aaaaacccug aaaacgcacc caucauaaga ucaauuaaaa uagaagaaaa agcgaacaga     2760

gucgucaugc uagaugagaa aaccgcagcc gacaacgcau cuauaguacg aacagaagua     2820

uucagacaca aagaaaccgg gaauuauuuu cuuacaccgg uuuaugucgc agaugucuug     2880

gcaaagaaag uaccugauag acuaauuacu acuaagaaac cauacucaga auggagaaaa     2940

aucacagacg aauaugaauu ucuguucucg cuguacccaa acgaccuaau acgaguaaaa     3000

augccaaaag aaaaaggaac aaaaacacau acuggugaaa aacacguaug gcaagaaaac     3060

uucauguauu uuaaaggagu ccauagugga ucugcaggag uaagaaugau agaccauaug     3120

aacucauucg aagacgaaau cggaguaaga aaauuagucg uuuuugaaaa auaucaggua     3180

gacccucuag guagaaugaa uaaaguucau ggcgagaaaa ggcacggaau auga           3234


<210>  5013
<211>  3234
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5013
augcaacaaa aaccacaacg uuauauacuu ggcuuagauc uagggauugc aagcguagga       60

ugggcuugug uaugggcaga cgaaaaugaa cauccaacag gauuguuaga uuguggagug      120

aggacauucg aaagggcaga aguaccuaag acaggagaua guuuggcuuu agcaagaaga      180

caagcaagga gcguaaggcg cuuaauacga agacgagcac auagauuauu aagacuaaga      240

agacuacuaa agagagaagg gguuuugcua agugcagauu uuaacgaaaa uggucuagug      300

aggggacucc cuaucgacgc cugggcacuc agaguggcag gauuagauag aaaguuaucc      360

aauaaagaau gggcagcagu acuuuuacac uuaguuaagc acagggguua ucuaagccaa      420

agaaagaaug aaacucaguc gaaagauaaa gaacuaggaa gauugcuagc aggaguaaac      480

ggaaacagag aagcacugca aaacagguca gacgaauaca gaacagccgc cgaacuagca      540

guuaagaaau ucgccgcuga aaauaacggg ucgcuaagga auaaaggagg agauuauagc      600

cacacauuug auagaaaaga uuuacaaaga gagcuacauc uauuauuuga acgucagaga      660

gcacuggaca auccauuuac aaguucagaa auggaagcag caguggauga auuauuaaug      720

aaacagagau ccgcacuuca aggugaagca gucuuaaaaa ugcuagguag auguacauuu      780

gaaccgagcg aauauaaagc agcuaaaaau acguauucag cagaaagauu uguaugguug      840

acaaaauuga acaauuuaag gauucaauau aauggagaag agagagccuu auccgaagca      900

gaacguaggc uucuuuuaga ugagcccuau caaaaagcua aauuaacaua uaaacaggua      960

agaacaaucc uagaacuucc agaaagugca gucuucaaag gguuaagaua cggccacgga     1020

gaagaugguu uaaaagcuga aaguggaaca cuaauggaga ugaaggcaua ccacgcgguc     1080

aggaaagcau uaaaagaugc aggacuugaa ggagaauggg aaaauaggau acggaaccca     1140

gaauuacucg augccauagg cacagcguuc agucuauaua aaacagacga agauauuaga     1200

gcaaauuuag cugcuuuuag acuuucagaa aauguacuug aagcguuauu agcaggauua     1260

aauuucgaua aauuuguuca ccucucucuc aaggcucugg cuaaaauauu accguuaaug     1320

gaacaaggaa ugcgauacga ugaggcaugc aaggaagcau acggagauca uuacggugcc     1380

aaagaacaag aagaucaccg acuauuacca ccauucguug cagacgauau aagaaacccu     1440

guuguuuuca gaacuuugac acaagcaaga aaaauuguaa augaaauagu uagaagauau     1500

ggauccccuc uuagaguaca cauugagaca gguagggaag ucggaaagag cuucagugac     1560

cgaaaagaaa uagagaaaag acaagaagaa aaccgaaaag aaagagaacg ugcagcagca     1620

cauuuuaaag aacuuuuucc uaauuucgcc ggugaaccga aggcacaaga cauacugaaa     1680

cugagauuau augaacaaca acauggaaaa uguuuguauu caggaaaaga aauagaccua     1740

agaagauuac cagaaaaagg guauguagaa aucgaucaug cacuaccauu uucgagaacc     1800

ugggacgaua guuuuaacaa caaaguucuc guauuaggua aagaaaacca agacaaaagg     1860

aacagaacuc cauacgaaua uuuagacgga aaaggcaaua guggagcaug gagagcauuc     1920

gcagaacgag ucucaggaug ucgauuuccg uaugcaaaga aacaacgacu uuuaacgcaa     1980

aaacuagaug agaaaacaga acaaggcuuu uuagaaagga aucugaauga cacacgauau     2040

gucgcgagau uuuuauguca auuuauagaa gagaaaaugc acuuggaagg ugcaggaaag     2100

aaaagaguau uugcuucaaa cggucaaaua acagcgcuau uaagagguuu cugggguuua     2160

aagaaaguaa gggaagaaaa ugauagacau cacgcucuag augcaguagu cgucgccugc     2220

aguuccguaa guaugcagca gaaaauaaca cgacauuuuc gauauagaga agaauuagaa     2280

cauauagaua gagaaacagg agaaguaauc cauggauuuc cacaaccaug ggaauuuuuc     2340

cgccaagaag ucaugacaag aguauuuagu gaugauccag uaucugaauu agcagaaaaa     2400

uuagacaguc gaccagaagc aguucaugaa uuuguaaccc cucuuuuugu aucaagagca     2460

ccacacagaa aaguaacagg ccaaggucac cuugaaacaa uuagaagugc aaaaagacua     2520

gaugagaaaa uaucaguagu aaaauuaccg uugacacaau uaaaaacaaa agacauagaa     2580

agaauagucg gauauccaaa cagagaacca gcacuauaug augcucuagc agcaaggcua     2640

gcagcauuua aagaugaucc agcaaaagca uuuagugaac cauucuauaa aacuggaaaa     2700

gauggaaaau gcgggguucu agucaaagcu guaagagucg aaaauguaca gaaaucagga     2760

cuuuugguaa gaaaagauga aagugguaaa augcaaggag uggcagauaa cgcaacgaug     2820

guucgcgucg auguuuucgg uaaaaacgga aagaauuacu uaguaccagu auaugcaugg     2880

cagguugaaa aaggugaacu uccaaaucgc gcaguaguag cauaugcaga ugaagaagau     2940

ugggaugaaa uggaugauag uuuugaguuc aaauuuaguu uauacccaaa ugaccuagua     3000

gaaguuguga caaagaaaga caaauucuuu ggauauuauu caggauucaa uagagcuacu     3060

ggugcuauaa acauuaaaga acaugauuua agcaaaagca aaguuaaaaa uggaguauac     3120

gaagguaucg ggguaaaaac ugcccaaagu uuucaaaaau aucaaguaga uacauugggu     3180

aaaacuaucc gauuaugcaa acccgagaaa agaaugggcu uuaaaaagaa auga           3234


<210>  5014
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5014
augagauaug uuuuaggucu agauauagga auagcuucau guggaugggc ugucauaaau       60

caagaaaaag aacgcauaga ggaccuagga guaagaauuu ucgacaaagc agaaaauccc      120

aaagacggca aaucacucgc agcacccaga agagacgcac gcucuacaag aagaacacuc      180

agaagaaaga aacaccgaau gcaacggauc aaagucuuac uuguaaaaca cggauuauua      240

ucaaaaacag agcuagacca uuuauacgaa ucagcaacug aaguagacgu auggaacuua      300

cgauuagacg ccuuagaacg uaaacuguac ccaaaagagu ucgcacggau acucauacac      360

cucgcgaaac gaagaggcuu caaaagcaau cgaaaagaaa cgacccucag cgaaaauggu      420

cagauauuag aaucuaucuc agaaaaucaa caaauaaugg aaaaacagaa cuaucgaacg      480

guaggagaaa ugauacugaa agacaagaaa uucgaaaauc auaaaagaaa caaaaaugga      540

acguacguag guacaguaac aagacaacaa cuccaagacg aaauucaaau gauauuccac      600

gcacaaagac uuuacaaaaa cgacuacgcu acagaggaau uugaaucuuc auacuuagaa      660

auaugggcua gucaacgacc auacgcaucg aaagaccaga uagagaaaau gauagguuau      720

uguacccuag aacccaaaga gaaaagagca cccaaagcaa gcuggucauu ucaauacuuc      780

gucgcucucc agacaaucaa uaacuuacga cugauaucaa aagaccgaau agaagaacua      840

ucauuugaag aaaagaaaca gauaaugaac uuagcacuug aaaaauccau aguaaaauau      900

auagauauca gaaaauuacu cucuguacca aacgaauuuc aauuuaacuc acucucuuau      960

uccgcagaua ccguagacac agcagucgaa ucacgaaaau guauagaauu aaaagaauau     1020

cacucgauca acaaaguaua uaaacaaaua uauggcgaaa augcauuaaa ccuucuuccu     1080

aaauacuacg auacaauagc guauggacuu accauauuca aagacgauaa agauauacua     1140

gaauacuuac aaaacaaaua uguaaacgca aaaggaaaac cuauaucaaa uuuugcaaau     1200

aaaacauaug augauacauu uauucaagcg cuacucaacu uaaacuuuac caaaauggga     1260

caucuaucau ucaaagcccu uaaaaacauu aucccauuuc uagaagaagg uuuaagcuac     1320

gacaaagcau gcgaaaaagc aggauacaac uuuaagggaa ccuccuacac agaacaaaca     1380

aaauaucuac cugucauacc ucaaaacaca aacccaguag uccacagggc acuaagccaa     1440

accaggaaag ugauuaacgc aauuauaaag aaauauggcu cuccaaacgc gauucacaua     1500

gaaacugcaa gagaacucuc uaaaacauuu caagaacgua aagaaaucga cucuauguau     1560

caagacaauu cuaagaaaaa ugaacacgca auccauaaac uaaaagaacu uggaaacaua     1620

aaccccucgg gaauaaacau cgucaaauuu aaacucugga acgaacaaga cgguaggugu     1680

auguacucag gaaaauauau cgagccacac agacuauucg aagaaggaua cacagaagua     1740

gaucacauuc uaccauauuc aagaucauua gaugauagcu auaauaacaa aacccugaca     1800

uuaggaacug aaaaccaacg aaaaggaaac aaaauacccu acgaauauau gggaaacaca     1860

ucuauaugga augauuucga aacacgagug caaucaaaca agaaaaucaa caaaaagaaa     1920

caacaaaaac uccuuuuaca acacuucucu uaugcucgag aacaagaguu caucgaaaga     1980

aaccuaaaug auacgagaua cgcuacuaua uacuuaagcu cacugauaca acaacaucuc     2040

aucuuuucag aaaguucaag gaaaaagaaa guacacacug uauccgguau aaucacauca     2100

caucuaagau ccagaugggg uuucaauaaa gauagaaaag aaggacacau acaucaugca     2160

cuagaugcug uaauaguagc uguaacaucc gaccacauga uccaaagagu aacaaaauau     2220

uauaaauuaa aagaacuuaa uagaaaucua caagcaaaaa gaaugccauu ccccgaaccc     2280

ugggaaggcu ucagacuaga auuagaagcc cgaauauccc caaauaccca acaguaccuu     2340

aaaggacuuc gauuuaaaaa cuaugcagac gucaaccuaa gcgaaauaaa aucaauauuu     2400

guaagucgca ugccaaaaca cucgauaacu ggagaacuac aucaagaaac aauaaggaaa     2460

uucauaggau auaacgaaaa agggaaagua uuaacagcca uuaaaacaaa acuagaagac     2520

aucccauucg augcaaaugg agacuucccc auguauggua aagaaucaga uuuauauaca     2580

uacaacgcca ucaaagaaag auaccuaucg cacaagaaag auaaaagaaa aucauuucaa     2640

gaaccacuuu auaaaccuac aaaauccgga ggaauaggac cucuaaucaa aucaauaaaa     2700

auaauggaua caagaacaau ugugaacccc gugaaucaag gaaaaggagu ugucuacaac     2760

uccaaaaucg cucgaaccga uguauuuaag aaagaagaga aauauuaccu uauucccaua     2820

uacacaauag auuuaaugaa aaacauccuu ccacaaaaag ccauaacagc aggaaaaggc     2880

uacgaagauu ggacaacaau agacccuucc uucacuuuuc uauucucauu auuccccaac     2940

gaucuaauac aaauuguccc uucuaaaaau aaaaccauaa aagcaagaac gaccguuucc     3000

aagaaagaag uauuacuccc aucacuuacc ggcuacuuua aaggcguuca uucaggaaca     3060

gcaggaauaa caguagaaac acaugacggg ucaguuauag caaaugucgg cagcaaacag     3120

uuacuuuuaa uagaaaaaua ccaagucgac gucuugggua acuauacuaa aauaaaagaa     3180

gagaaacgca ucgguuuaac aacauauaau gauaacaaga aaauagcagc uugcuga        3237


<210>  5015
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5015
augaaacaac aaccacaaag guauauucua ggccuagauu uaggaauugc aucaguaggg       60

ugggcacuaa uacuuguaga ugaagaaggc aaccccauag gacuacuaga cuguggcgua      120

agaacauucg aaagagcaga aguucccaaa acaggcgauu cgcuagcacu ugcaagaaga      180

caagcaagaa gcguaagaag guuaauccgu cgcagagcuc acagauuacu aagacuuaga      240

agacuucuaa aaagagaagg uguauuguca gcagcugacu uuaacagcga cggauuaccu      300

aaucuaccaa uagacacaug gcaacuacga gucgcuggcc uugaaagaaa acuaacaaau      360

caagaguggg cagcaguccu uuuacaucua gucaaacaua gaggauauuu aucccaacga      420

aaaagcgaaa cacaaacaga aaacaaagaa uugggauguu uacuugcauc aguugccaaa      480

aauucaaaau ugcuuucgga aaaugcaaac caauaucgca cuccggcgga guuagcaguu      540

cgccuauuug ccgaacaauc aggucacuua agaaauaaag gaggagauua uacucauaca      600

uucaaccgac uagaucuaca aaaagagcuu caccucuuau ucgaacaaca gcgaacauuu      660

ggaaaccccu ucgcuagugc agaauuacaa ucaaaagugg auggccuuuu aaugaugcag      720

cgcagugcac uacaaggaga agcuguacua aaaaugcucg ggaaaugcac guucgaacca      780

agcgaauaca aagccgcuaa acacacauac ucagccgaaa gauucauaug guuaacaaaa      840

cuaaauaauu uaagaauuuu agaaaacgga acagaaagag cgcuuaauga agcggaacga      900

aaauuauuac uagaugaacc cuacaagaaa gcuaaacuaa cguauagcca ggcacgcaaa      960

uuauuagaua ugagucacga ugcaacauuu aaaggacuaa gauauggaaa agacucggaa     1020

ggccucaaug cagaaaacgg ugcuuuaaug gaaaugaagg ccuaccauaa aauacgaacu     1080

guucuagaua aagcaggacu caagggagaa ugggaaaauc gcauuagaaa uccagaaauu     1140

uuagacaaga uaggaacagc auuuucacuc uauaaaacag augaagauau acaagcagcg     1200

cuugcagauc uuagacuucc agaaaaugua cuuaacgcac ucuuagcagg acuaaacuuc     1260

gacaaauuca uacaacuguc acuaaaggca uuaucaaaua uaaugccauu aauggaacgu     1320

ggaauaagau acgacgaagc cuguaaagaa gucuacggag accauagagc ucuagcagaa     1380

agcaaaaacc aaauguuacu ucccccaaua ccggcuaaug agauuagaaa ucccguugua     1440

cuccgaaccu uaacucaaac acguaaagua auaaacgcaa uaauucguag auauggaagc     1500

ccgcaacgug uacacauaga gaccgcaagg gaacucggua agagcuucaa ggauagaaaa     1560

gaaaucgaaa aacgucaaga agaaaacaga aaagaaaggg aaagagcggc agaacauuuc     1620

aaagaacucu ucccucauuu cgcaggggag ccuaagggug gagauauacu uaaacuaaga     1680

uuauaugaac aacaacaagg uaaaugcuua uauucaucca uaccaauaga ucuuggaagg     1740

uuaaaugaaa aaggauacgu cgaaauagac cacgcuuuac cuuuuagucg aacaugggac     1800

gauaguuuua acaauaaagu auuaguauua gguucagaaa aucaaaagaa aagaaaucaa     1860

acaccauacg aauaucucga cggggcuagc aacagucaaa gguggcggga guuccaagca     1920

cgauuacaag gaugcagauu uucauaugca aagaaacaaa gaauccagac agcaaaacuu     1980

gauaguaaaa cagagcaagg auuuuuagaa agaaacuuaa augauacuag auauauagca     2040

cgguuccuau gcgguuuuau ugaggaaaac cuacacuuac aaggggaagg uaaaagaaga     2100

guauuugccu ccaauggcca aauaacagcu uuacuaagag gacuaugggg cuuaaagaaa     2160

guaagagaag aaaaugaucg acaucaugca uuagaugcaa uaguaguagc auguaguacu     2220

gucucaaugc aacagaaaau aacaaaagcu augcaaagaa gagaaacuuu agaaacagua     2280

gauacagaaa caggagaagu aaaaacacga auaccacaac caugggacuu cuuucgucaa     2340

gaagucauga ugagaguauu uagcgauaca ccccacgaag aacuggcgga aaaauuaucu     2400

ggaagaccug aagcauuaca ugauuucgua aguccacugu uugucucacg agcaccuaau     2460

agaaaaauga cgggacaagg ucaucucgaa acaguuaaau cugcaaaaag auuaagugaa     2520

aauauuuccg ucgugagaaa accacuaacu gcauuaaaau uaaaagauau cgagaauaua     2580

gucggauauc cagauagaga accagcauua uaucaagcgu uacgugaaag auuacaagca     2640

uacaaagaug auccaguuaa ggcauucgcc gaacccuuuu acaaaccugc caaaaauggu     2700

gggcaaggau cucuagucaa agcagucaga guaaaggacg uacagaaaac aggagucaug     2760

guuagaaaag aucgcggugg cauugcacga ggaauagccg auaaugccac uaugguaaga     2820

guagauguau ucgguaagaa agguaaaaac uaucuaguuc ccgucuacgc uuggcaaguc     2880

gcaaagggua uauuaccaaa cagagcagua guagcauacg cugaugaagc agacugggau     2940

gaaauggaug acucauauga guucaaauuu acguuguauc caaacgaccu ggucgaaauu     3000

auaagcaaga aaggaaggau auucggauac uauaguggau uaaaucgagc aacaggcgca     3060

auagauauaa aagaacauga ccuaucuaaa gaaaaagcua aaaaugguau acaucagagc     3120

guaggaguaa aaacuguacu aucauuucaa aaauaucaag uagauccacu ugguaaagaa     3180

aucugcuugu gcaaaguaga aaaacauccc gaguuaaaca gcaaaaagaa gaaauga        3237


<210>  5016
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5016
augggauacu uaaucgguuu agauaucgga auuacaagug uaggauacgc aguacuccaa       60

accgacuuua aagggaaccc guacaaaauc uuacucauga auacagucau auucccaguu      120

gcugaagcac cuaaaacugg ggaaucacuu gccgcaccug ugagacagaa acgauccucu      180

cgacgcacua acagacgaac aaaauuccgc aaauugcgag uacaaaaacu auucguccga      240

uucggacucc uagaauccgc ugacguugca acauuucuaa aucaaaaagu cccccaucaa      300

gauaucuggc aauuaagaaa agacgcucuc aaccgcaaau uaucaaacag agaacuauuc      360

gaaauacuau acuuuuucgu cggacaucgc ggauuuaaau cuaacagaag aagugaagua      420

acagguaaac aaccaaaaga uaaccaagua guacucacgu cgauccauga ccgcggcaac      480

acuuuaaacg auuucgacuc gcucggagaa augaugcaag aauuacccga guucaaaaca      540

auaaaacaua acaaagcaua ccguccagac aauacuauau acccauuacg ugcauggauc      600

gaacaugaac uuucugccau auucucaguc caacagaaaa acaauagaca aaucaccgau      660

aauuuuauaa cucaauaccu ugauauauuc agaucacaaa gagauuucga ccaaggccca      720

gcagcuccaa guagauucgg aggaaacaug auagaaagaa ugguaggacc ggauucucuc      780

gacgcccacg agaaaagagc agccaaauua accaacacau ucuuuagauu uaacuuucua      840

ucaacacuua acaaucuuaa aauccaacuc acacuuggcg ccgaauuuca cccacucaca      900

acaccccaaa gagaaauagu acuccaagca gcgacccuca aauccuuaaa cuacaaacaa      960

aucagacaua aacuacaauu accugacuca gcccgauuua accucguacc auaucaaucu     1020

aacgaagacc caauaaaaac agaaucaaaa cauuauuugu ucaaacgaaa agccuuaaca     1080

caaauagaag ccguaaccga caacccacaa cuaaucgaua aaaucggcac aauacuagca     1140

uauuucaaag gagacaaaac ccgacaggaa aaacugagcg cacucaaccu cucaagagca     1200

gaaauaaaac aacuuuugcc aaucaaccaa ccaggauucg guaaucuauc auuaaaaaca     1260

augcacaaaa uucuaccaua uuuagaacaa ggccaucuau accaugaagc agcacaacug     1320

gcuggcuaug acuuucaaaa caaccucguu gacaggcaau accuccggga uaaucucaaa     1380

aaccccguag uaaacagagc caucggaaaa acaauaaaag uaguaaacgc aauuauaaag     1440

aaauacgggg aucccgacgc cauacauauu gaacuaacac gagaaaucaa acacucucac     1500

gaagagagac agaaaauagc aaggcaaaug aauaccaaca uggaucaaaa ugauaaaguc     1560

gccgaacauu uaagagaaaa cggaauaccc guaaaugguc aaaacauaau uaaaucaaaa     1620

uuauucuaug aacaaaaagg aauagaucua uauaguggac aacaaaucaa cgaacaagac     1680

uuauauacug aagaccauua ccaaguagau cacauuauac cauauucaau aucauucaau     1740

gauagcuuca ccaacaaaac ugucacgcga acaucuaaca aucaaaacaa agcaaaucgu     1800

uuaccacuag aauaccucag cgaagagaaa caagaugcau uuagagcaag agucaugagc     1860

aauauaucaa acuacagaaa acgacaaaac cucuuaaaag aagguauaac ugaagauaac     1920

cgacaacaau ggaaaacacg uaauaucaau gauacaggau acauaaaucg auuacuaucc     1980

caauaucuca gaaacaaugu acaauucacu aaaaagucuc auaacccugu guuuacaguc     2040

aauggagcaa ccacagcuua cauuagaaaa agguacggua uaauuaaaaa uagacaagca     2100

acagaccuac accaugcagu agaugcagua aucaucggcu guaucucaaa cacauucgua     2160

ucaagacuug ccaaauauuc ugaagcacaa gaagcacgau aucacaaaca acuauggcaa     2220

auaacacaau auagacaaga acauaaagac uuaucaacca uagaguacca agaacuuaua     2280

aaacaauucc cacucccaug gccagacuuc cgacaugagu uaauggcucg ucuaaguaca     2340

gacccagcaa aaaugauggc ucaucacaca ugggaacacu acacagauga agaaauaagc     2400

caacuaaaac cagccuucgu aauaagaucu gcuaaccaca aagugacagg aucugcucac     2460

gaugacacaa uuuacagcaa gaaacuauau gaccaacgug gaguaggcuu acuucgaaca     2520

ucucuacaaa aucuaaaaua cgacagcaag aaagacggaa uuaaaaacua cgaacggcua     2580

aaagauggca gcaacagucu agucuaugac ucauuacucc gugcccucaa agaacacgac     2640

ggaaacgcca agaaagcauu cccggacaac caacuacuaa uuucgacagg aaaccaucag     2700

caaauuauaa cacacgucaa aacaguaaag aaaauaaccc acccuauaga aguaaaucga     2760

gacacagcua uugcaauagg aggaagaaug aucagagucg acauuuucaa acaagcaaaa     2820

gcccaaaaau uaauuggaau accaguauac guaaaugaca cagucaaacc aacccuacca     2880

aacagagccu gcaccagugg uaaaagauau ucagacuggu aucaacucac aagacaagau     2940

caauuucuuu gcuccuuagu gucaaaugac cuaguccucg uagaaacugc cucuccauua     3000

aaagucacga acaacauuac aaacgaagaa guacaaacua cucaacuacu guguuacuuu     3060

ucuagcuuua auauagccaa cgcacaaaua aacuuccaua guauuaauaa ugacuccuca     3120

aucaauccaa uaguacuaaa caaaguaaca uauuuaacaa aauaucaauu agacuauuua     3180

ggaaacuacu uuccaguaaa acaugaaagc agaaaaacuu ucaauagaaa aagauga        3237


<210>  5017
<211>  3237
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5017
augcacacaa gacuagcauu agauauagga acaaccagua uaggauggau ccuauaugaa       60

uugguaaacg gacagccagc guccauauua gacggaggaa cucgaauuuu cagugauggu      120

agagaagcga aaacaggaca uucuuuagcg gucgggagga gaacagcccg agguaugaga      180

cgucgacgag aucgguacuu aagaaggcgu gcagcauuga ugaaaagacu agcagcaaua      240

ggcuuaaugc cagaaucucc agaagcugca gcucaacuag caacauuaga uccauaugaa      300

uuacgagcua gaggauuaga ccauccauua ccaccagagc auuuaggacg agcuuuauuc      360

caccuuaauc aaagaagagg auucaagagu aacagacgaa cagauaaagg aaauaacgaa      420

ggggguauga uugcggacgc gucagcacgu uuagagcaag caaugauggc agcuggugcc      480

cguaguuacg gcgaauuuuu acacaagaga cgagcagaag cgacggcucc aagagccacu      540

cccgcaguua gaacaagaau uaauccgcau cugacuggag aagaugguaa ggaacaacca      600

gguuacgauu uuuacccaga uaggaaacac cucgaagacg aauuucauag auuaugggaa      660

gcucaagcua gacauaaucc agaucuaacu ccagcaaugg gggauuugcu uuuucaaacu      720

auauuucauc aaagaccucu caaggcgccu gcaguuggaa gauguuuauu uuuggaugaa      780

ccaagauuac caaaggcaca uccacuaaca caacagagga cacucuauga gacagugaac      840

auguuaagga uugcaccacc aggucaaccu aaaagacguc uuucuagagu acagagagau      900

caaauaguac acgcuuuaaa uaauagaaaa auacuagcau caccaggaaa gaacguuuua      960

accuuagcag ccauaggaaa acuacuaaaa uuacgaccag gagaagcauu cagcuuagau     1020

acugcaaaca gagacgcaau ugccugcgau ccuguacgaa ugaguuuagc auaugaagga     1080

agauuugguc cacaguggag ugcauuauca guagaaucac agugggaauu aauacagcgu     1140

cugagagaug aucaagaugg agcagcacuc guugacugga uaauggaucg cuacggauuc     1200

gacagagaua gagcaagagc aauugcagac gcuccucuac cggaaggcuu uggacgacuc     1260

ggugagacgg caacaagagc aguuuuggcu guauuaauag gggguaccac agagcaagaa     1320

guuuuaaccu acagugaagc cguagcugca uguggauggg aucauucgcg gcuaggaaca     1380

ggggaaguuc uagauagacu uccauauuau ggacaaguau uagacagaca cguucuaccc     1440

gguacgggca gaccagaaga cgaugaaaua acaagauucg ggaggauaac gaauccaaca     1500

guucauauag ggcuagggca aguaaaaaga cuuguuaaua aaguaauuga cgccugggga     1560

cgaccacacg aaaugguagu ugaauuagcu agggauuuaa aaaugaguga agaucaaaaa     1620

cgcaauuuag caaaaguaaa cgcccaaaac caacgagcag cagaaaugcg auccgcaaaa     1680

cuuauaggcg auuuaaaaau agaagacaau gguaggaaua gagguauacu caggguaugg     1740

gaagagagug cagccgaugu auuacacaga uguuguccau auacaggagc ccaaauaagu     1800

gcguccaugc uauuugaugg aucaugugau auagaucaca uauugccaua cucaagaaca     1860

cuugaugaug guuuuaacaa uagaguuaua uguuuaaaaa gugcaaaccg ggagaaaaga     1920

aaccuuaccc caugggaagc auggggaaau acaccuaaau gggcugcaau agaagcaaau     1980

uuaaagaauu uaccaccggc aaaagccugg agauuugcuc cagaugcaau guuaagauau     2040

gaaggagaaa gagacuuuac agcaagagca uuaguagaca cgcaauacuu aucaagacua     2100

gcccgagaau accucgagug uuuauaugcg gaggacauag auccugggcc acauuccggu     2160

aagaaaccua gaagaccuau cuggguagug acgggaaaac uaacugaacu ucuacgaaga     2220

cauuggggcu uaaauagcuu auuaccagau gcucacagag gaaccgcuaa agccaaaaau     2280

agauuagacc auagacacca uaugauagau gcagcaguag uaggagcaac cgaucacgca     2340

acaguacaaa gaauagcaaa uauggcaaaa ggaaauguug aaaauggcuu agaagaccua     2400

gcuggaacca uacuaccacc auggccggga cuaagagaug aucuaaaagc uagacucgaa     2460

agaacaauag ucucacauag aggcaaccac ggagcaaucg acccugcagc ucgaaaacaa     2520

ggacgagaua cgacaucagg acaacuacau aaugacacgg cuuauggauu aacaggagaa     2580

acauuaaaag gagucccccu uguaguuaau agaaaaccaa uagacauguu aacacccgcc     2640

augauagaua aaauuagaga uccacauuua agccgguggc uaacugcugu aacgaaaggc     2700

aaagaaggaa aagauuucaa agcugcggua gaagcauuuc gagcccaucc aggacccuau     2760

agaggaauca ggcguguaag auugauagaa cccguagaag uaauuagaau aaaagauaga     2820

aaugggagac cguauaaagg guauaaaggc gacagcaauc aguguuauga aguauggugu     2880

uuacccgaua gaacauuaac acaccauguu guaaguacau uugcggccca ccaaggacaa     2940

acugggaaac cacacccagc agcuaaaaga uuagcacgau uauauaaagg agacauggua     3000

agacuagauc gcucaaaauu cggaucagug auugcaacag uagaaaaauu ugauagaaac     3060

ggucagcuag agcuaguacc ccacaaugaa ggcaaugcag aucaacgaua uagaaaagau     3120

aaaagugauu caguauucau uaggcuacga gcagccaccu ucauagcgag cggugcaaga     3180

cgcguaauag uugaugagau ggggaggaua cgggacccug guccagggaa gcauuga        3237


<210>  5018
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5018
augaaauacg gaauaggacu ggacugcggu auaaauucag uuggauacug uauaauggag       60

cuuaacucug acgacgaacc uaaaagaauc guaagacuua acucaagaau cuucacugaa      120

gcggaaaacc caaaagacgg uuccaguuua gccgcaccaa gaagagaagc aagaggauua      180

aggagaagaa uccgaagaca caaacaccgc cucgaaagaa ucagagccuu auuagucaga      240

gaaaacauag uaacacaagc ggaguuagac aauauguucu caggaacucu aucagacauu      300

uaugaauuaa gaacagcagc cuuagacgca ccuuuaaaca acauugaauu ugcacgcgua      360

cucauaaacu uagcucaacg aaggggauuu aaaagcaaca gaaagucaga aaaaucaucc      420

agcgacaaag aaaccggcaa acuguuaagc gcuauaaaaa aaaauaaaga gaguauagaa      480

gcaggaggau accgcacagu aggagaaaug cucuuuaaag augaaagaua ugccaagcac      540

aaaagaaaua aaggugaagc uuaccuaaac accgugagcc gagacaugau cgaggcagaa      600

auccacgcag ucuuugccuc acagagaaau uuuggaaugc cauuugcaaa cgaaaaaauc      660

gaaaacgaau auacagacau cgcauugucg caaagaccuu ucgaccuggg cccaggagaa      720

ggacccgaaa auuguaaauc uccauacgca ggaaaacaaa uaaaaaauau gauuggccaa      780

ugcaccuuuu uuccagaaga aaaaagagca guaaaagccu ccuacucauu ccaacuguuu      840

agucucuugc aaggaauaaa uaauauaacu cuugucuccu cugacggaaa uacuuuccca      900

cuaagucaag aacaaagaaa aacucuuaaa gacuacugcu uuaaaacagc aaauguaaca      960

uaugcaagua uacgaaaggu acugcaaaua ccucaagaau acacuuucaa aaauauaaca     1020

uacacagacg auggaaucga aaaaagcgaa aaaaaaacca aguuccaaca ccucaaacca     1080

uaccaugaaa ugaaaaaggu acugggauca gcaauacaaa cacucacacc agaagaacuu     1140

gaugaaauug gagaaauauu uacguucuau aaagacgaaa caaucauaau caaacaacua     1200

gaagaaacag aaauagacaa aucauuauau gauuuauuac uccaacuacc cacauuugaa     1260

aaagcgggac acauaucuac aaaagccugu aagaugauaa uaccauuuuu agaagaagga     1320

aaaacauaug acaaagccug cgaaugcgca ggucucaacu ucaaagcaca cacaaauaug     1380

cagaaacaaa ugcuucuacc ugcaaaaucu gacgaacuaa acgacguagu aaacccagua     1440

guccgaagag caaucagcca aacgaucaaa guaguuaaug cuauaauacg agagaugggu     1500

acuucaccua cauacauaaa cauagaauua gcaagagaac uuagcaaauc aaaaaaagaa     1560

agagaugaaa uaaacaaaaa auauaaauug aaucaggcag aaaacgaacg auuaaagaau     1620

gaaauaauag acaacuucaa aauaaaacca aacggacaag acauaauaaa acuaaaacuu     1680

uggcacgaac aggacgaugg aauaugccca uacaccggcg ucagaauaaa auaugaaaac     1740

uuauuugaug acggauacgc agaaauugau cauauaauac cguacuccuu aaguuuugac     1800

gacucauuua auaacaaaau ccuuacaacu gcaacccaaa auagacaaaa aggcaaucga     1860

auuccauugg aauaccuccc cgaaucagaa aaagauggcu ucagagucug gguuaacagc     1920

aacauacgca accauagaaa acgacaaaac cuucuaaaag aacacuuuac agaagcagau     1980

aaaaaagaau uuaaaagcag aaauuuaaau gauacccguu acuuaaacaa acuauuguua     2040

aacuauauca acgacaaccu auuauucacu gauuucuaua acggaaaaaa aaagcaugua     2100

acaucaguua acggggcagu aacagccuac aucaggaaaa gauggggaau uaauaaaaau     2160

agagaagaug gcgaccuaca ucaugcagua gaugcagccg uaaucgccug uguaacucaa     2220

ggaauaauaa acagaauaac ugcauauagc gcaagaaaug aagcaccaua cgaucugaau     2280

accgaaacug gugaaauaaa ggagcgauuc cccuuaccau accccaauuu caaaaaggaa     2340

cucgaugcua ggacaacuau ugaagaugaa aaaagguuaa aaaggguucu aaugagcuuu     2400

ucaaacuauu cauaugacga cgucgaaaag gccaaaccgu guuucguguc uagaaccccc     2460

cauagaaaag uaacaggagc agcacacgaa gaaaccauuc gaagcggcaa agucgaagga     2520

uuuacuguaa gcaaaacuga acugacuaaa cuaaaguuaa caaauggaga aauugaagga     2580

uauuacgaac cagaaucuga cacacuacua uacaaugcgu uacuucaaag acuuaacgaa     2640

uucggaggug augcaaaagc ggcauuugcc gacgaauucc auaaaccaaa agcagacggg     2700

acucccggac ccguaguaaa aaaaguaaag acaauaaaaa gauccucguc uaacguuaaa     2760

gcccgaaaua agaacggauu aggagucgca gacaauggca guaugauaag aaucgaugua     2820

uuuuauguag aaaaugaagg cuacuacuuc gucccaaucu auguugcaga cacaguaaaa     2880

gagaugccaa augaggcaug uccuuucggu aaggauaaaa aaaaaauuau gacggacgac     2940

gacuuucuau ucagcuuaua uccaaaugau cuaauaaaaa uuacagcuaa aaaagacaug     3000

aaguucagcc uugcgaacaa agaauccgga cugccaaaaa auaaauacgc uaaugaagua     3060

uuaguauauu acucagcagc agacauuagc aacgcaagca uuucugucau aaugcaugau     3120

aaugcauaca aauucagagg auguggaauu aaaucauuag uuaaacuaga aaaauacacg     3180

guggacccuc ucgguaacgu aucuaaaguc aauagggaaa aaaggaugua uuuuaaauga     3240


<210>  5019
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5019
augagacuag gccuugauau uggaaccuca uccauaggau ggugguuaua ugaaacugau       60

ggggcuggau cggaugcaag aauaacaggc guagucgacg gagguguaag aauauucuca      120

gauggaaggg acccaaaaag cggcgcaucg uuagcuguag auaggagagc cgcaagagca      180

augagaagaa gaagggaucg auaucuacga cgccgugcaa cauuaaugaa agugcuagca      240

gaaacgggau uaaugccugc agacccugca gaagcaaagg cuuuagaagc acuagacccu      300

uuugccuuaa gagcugccgg ucuagaugaa ccuuuaccac ugccucauuu ggguagggca      360

cuauuucacu uaaaucaaag gcgaggcuuu aaaaguaauc gaaaaaccga cagaggugau      420

aaugagagug ggaaaauuaa ggaugcaacu gcuagacuag auauggaaau gauggcuaau      480

ggagcucgga cauaugguga guuccuucac aaacgaagac aaaaagcaac agauccucga      540

caugucccua gcgucagaac cagauuauca auagcuaauc ggggaggccc ggacggcaag      600

gaagaagcag gauaugauuu uuauccagau cguagacacu uagaagaaga auuucauaaa      660

cuaugggcag cacaaggagc ccaucaucca gaauuaacag aaacgcuaag agacuugcua      720

uuugagaaaa ucuucuuuca aaggccacua aaagaacccg aaguuggauu auguuuauuu      780

ucugggcauc auggaguacc acccaaagau ccccgucugc caaaagcaca cccucuaacu      840

caaaggagag uccuuuacga aacaguaaau caauuaagag uuacagcuga cggaagagaa      900

gcucgcccau uaacuagaga agaacgagau caaguuauuc augcacugga uaacaaaaag      960

ccaacgaaau cucuuucguc aaugguacua aaacuaccag cuuuagcaaa aguauuaaaa     1020

cuacgagacg gagaaagauu uacccuagaa acuggagucc gagacgcgau agcaugcgau     1080

cccuuacgug caagcccggc ccauccagau agguuugguc caagguggag cauuuuagau     1140

gcggacgcuc aaugggaagu aauuaguaga auacgaagag uacaaucaga ugcagaacau     1200

gcugcccuag uugacugguu aaccgaagca cacggauuag acagagcaca cgcagaagca     1260

acugcucacg cuccauuacc agauggauau gggagauuag gauuaacggc aacaaccaga     1320

aucuuauauc aacuaacugc agacguagua acauaugccg acgccguaaa agcauguggc     1380

uggcaccauu cagauggacg aacaggcgaa uguuuugaca gguuaccaua cuacggugaa     1440

guauuagaaa gacacgucau accgggcucg uaccauccug augaugauga uauaacuagg     1500

uuuggaagaa uaaccaaccc gacaguacau auaggacuaa aucaauugag aagacucguc     1560

aauagaauaa ucgaaacaca cggaaaacca caccaaaucg uaguagaauu agcaagagac     1620

cuaaagaaau cagaagaaca aaagagagca gauauaaaac gcauaagaga cacuacagaa     1680

gcagcaaaga aacgcagcga gaaacuagag gaauuagaga uagaagauaa cggccguaau     1740

cgaauguuau ugagauuaug ggaagaccua aauccagacg augcuaugag gagguuuugu     1800

cccuacaccg guaccaggau aucagcagca augauauucg acgguucaug ugauguagac     1860

cauauauuac cauacagcag aacgcucgau gauucuuuuc ccaauaggac acuuugccuu     1920

agagaagcaa auagacagaa aagaaaucaa accccauggc aagcaugggg ugauacgccu     1980

cauuggcacg caauagcugc aaauuuaaag aaucuaccag agaacaaaag auggagauuu     2040

gcaccagacg caaugacccg guuugaaggg gaaaauggau uuuuagauag ggcauuaaaa     2100

gauacucaau aucuugcaag aauaaguaga agcuaccuag auaccuuauu uacaaaaggu     2160

ggucaugugu gggugguucc aggaagguuu accgagaugc uuagaagaca cuggggauua     2220

aauaguuuau uaucagaugc gggucgaggg gcuguaaaag ccaaaaaucg cacugaucac     2280

agacaccaug ccauagacgc agcagucauu gcagcaacag auccaggacu auuaaacaga     2340

auaucaagag cagcagguca aggugaagca gcaggacaaa gcgcagaacu uaucgccaga     2400

gauaccccac ccccauggga gggauucaga gaugaucuca gaguaagauu agauagaaua     2460

auaguaagcc aucgggcuga ucaugggagg auagaucacg cagccagaaa acaaggacgc     2520

gauuccacag cgggacaacu ucaccaagaa acggcauaua guauaguuga ugacauacau     2580

gucgcaucaa gaacagaucu ccuaagucua aaaccagcgc aauuauugga cgaaccagga     2640

aggaguggac aaguacgcga cccucaacua cgaaaagcau uaagaguggc caccggaggu     2700

aagacaggca aggacuuuga aaaugcucug agauauuuug ccagcaaacc cggcccauau     2760

caagcgauac gaagagugag aauaaucaag ccauugcaag cacaagcaag aguaccaguu     2820

cccgcacaag aucccaucaa agccuaucaa ggugguucua aucaucuuuu cgaaauuugg     2880

aggcuaccag auggugaaau agaagcgcaa guaauaacau cguuugaagc acacacuuua     2940

gaaggagaga aaagaccaca cccugcugca aaaagauuac uaagaguuca caaaggugau     3000

auggucgcac uagaaaggga cgggagaaga guagucggac acguacagaa aauggacaua     3060

gcaaauggau uauuuauagu cccacacaau gaagcgaaug cggauacccg uaauaaugau     3120

aaaucagauc cuuucaaaug gauacaaaua ggagcaaggc ccgcaauugc aucaggcaua     3180

agaagaguaa gugucgauga aauaggaaga uuaagagaug gaggaacaag accaaucuga     3240


<210>  5020
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5020
auggacgaag aagcgaaaau acaauuaagu aaagaaucuu augaagaaga acucgaaucu       60

uuacuagaga aaaguggaga agaauuuaga gauguguucc uacaagccaa aaagguauau      120

gacgcaaucu uguuaagcga cauacuaagc acaaagaaac aaaauucaaa agcaaaauua      180

uccuuaggaa ugaucgaaag auaugacuca cauaagaaag aucuagagga acuaaaacaa      240

uuuguaaaag caaaccuacc agagaaaaca gccauauuuu ucaaagacag caguaaaaac      300

ggauaugcag gcuacauaga cggaaagacg acucaagaag acuucuacaa auuucuaaag      360

aaagaacuaa acggaaucgc uggcucagaa cgcuuuaugg aaaaaguuga ccaagaaaac      420

uuccuccuca aacaacgcac aacagcuaac ggcguaauac cacaccaagu gcauuuaaca      480

gaacucaaag ccauaauuga aagacaaaaa cccuacuacc cauucuuaga agaggcacgg      540

gauaagauga uaagauuacu aacuuuuaga auaccauauu acguaggacc guuagcacaa      600

ggugaagaaa caaguucguu cgcaugguug gaaaggaaga caccagagaa aguaacacca      660

uggaacgcaa ccgaaguaau agauuauagc gcaucugcaa ugaaauucau acaaagaaug      720

auaaacuaug acaccuaucu accaacugaa aaaguccuac caaaacauuc uauauuguac      780

caaaaauaua caauauucaa cgaacuaaca aaaguggcau acaaagacga acgaggaaua      840

aaacaccaau uuucgucaaa ggaaaaacga gaaauauuua aagagcuauu ccaaaaacaa      900

cgaaaaguga ccgucaagaa auuacaacaa uuucuaagcg cgaacuauaa cauagaagac      960

gcggagauuu uaggcgucga caaagcauuc aacuccucau augcaacaua ucacgacuuc     1020

cuagaccuag ccaaacccaa cacagaacgu gucgcagaau uacuugaaca accagagaug     1080

aaugcaaugu ucgaagacau aguuaaaaua cucacaauau uugaagauag ggaaaugauc     1140

agaacacaac ucaagaaaua ucaaagcgua uuaggugaug gauucuuuaa gaaauuagua     1200

aagaaacauu acacaggaug ggguagauua agugaaagau uaaucaacgg caucagagac     1260

aagaaaacua acaaaacaau ucuagauuac cuaauagacg acgacgacuu cccauauaac     1320

agaaauagaa acuucaugca acuaauaaac gacgauucac uaucauucaa agaagaauua     1380

gccaacgaac ucgcauuggc aggcaaccaa aguuuacuag aaguaguaga agccuuacca     1440

gguucaccag ccauaaagaa aggaauuugg caaaccuuga aaauaguaga agaacucaua     1500

gaaauaauag gauacaaucc gaaaaauaua guaguagaaa uggcacguga aaaucagaga     1560

acuaacagaa gcaaacccag acuaaaagcu cucgaagaag cacucaaauc guuugauagu     1620

ccccuccuga aagaacaacc agucgacaac caagcccuac agaaagacag guuauaccuu     1680

uauuaucuac aaaacgggaa agauauguac acaggugaag cccuagauau agauagacua     1740

uccgaauacg acauagacca cauaauacca cgaaguuuca uagucgauaa cagcauagac     1800

aacaaaguuc uaguaucaag uaaagaaaau cgauugaaaa uggacgacgu uccugaucaa     1860

aaagucguca uccgaaugcg aagauauugg gaaaaacugu uaagagcaaa ccuaaucagu     1920

gaaagaaaau ucgcuuaucu uacaaaacuu gaguuaacac cugaagacaa agcacgauuu     1980

auucagagac agcuaguaga aaccagacaa aucacuaaac acguagccgc cauauuagac     2040

caauauuuca aucaaccaga agaauccaaa aacaaaggaa uacggaucau cacucuaaag     2100

ucaagccuag uaucacaauu cagaaaaaca uucggcauac auaaaguuag agaaauaaac     2160

aaucaucacc acgcacauga ugccuaccua aauggcguag uugcaauagc guuacucaaa     2220

aaguacccaa aauuagaacc cgaguucguc uauggaaauu acacaaaauu uaauuuggca     2280

acagaaaaca aagcaacagc caagaaagaa uuuuauucaa auauauuacg auuuuucgaa     2340

aaagaagaau acagcuauga cgagaaugga gaaauauuuu gggauaaggc aagacacaua     2400

ccucaaauca agaaaguaau aaguagccac caaguuaaua uagucaagaa aguagaaguc     2460

caaaccggag gauuuuauaa agaaacagua aacccaaaag guaaaccaga uaaacuaauu     2520

caaagaaaag cggguuggga cgucagcaaa uacggaggcu ucgguucacc aguaguugcc     2580

uacgcagucg cauuuauaua ugaaaaaggu aaagcacgaa agaaagcaaa agcaauagaa     2640

gguauaacaa uaaugaaaca aucauuauuc gaacaagacc ccauaggcuu uuuaucaaac     2700

aaaggcuauu caaacguuac aaaauuuauu aaauuaucca aauauacacu cuaugaacuu     2760

gaaaauggaa gaagacguau gguagcaagc cacaaagagg cccaaaaagc caauucauuu     2820

auacuuccag aaaagcuagu gacuuuacua uucuacgccc aacacuacga ugaaauagca     2880

cacaaagaaa guuuugacua cgucaacgac cauuuaagcg aguuccguga aaucuuagac     2940

caaguaauag auuucagcaa uagauacacg auagccgcga aaaacacuga gaaaaucgca     3000

gaauuauuug aacaaaacca agaaucaacc guacaaucau ugagucaauc auuuauaaau     3060

cucaugcaac uuaaugcaau gggagcccca gcugauuuca aauucuucga cguaauaaua     3120

cccagaaaaa gauauccauc cuuaacagaa auaugggaau caacuauuau auaccaaagu     3180

auaaccggac ugagggaaac ucgcacaaga auggcgaccu uaugggaugg ugaacaauga     3240


<210>  5021
<211>  3240
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5021
auggacgaag aagccaaaau acaacuaucg aaagaaaguu acgaagaaga auuggaauca       60

cugcucgaaa aaucagguga agaguucagg gauguuuucu uacaagcuaa aaagguguau      120

gacgcaauau uauuaucaga uauacuaucg acgaagaaac aaaauucaaa agcaaaacua      180

ucauuaggua ugaucgaaag auaugacucg cacaagaaag auuuagaaga acucaaacaa      240

uucgugaaag caaaucugcc agagaaaacc gcaauauuuu ucaaagacuc caguaaaaau      300

ggauaugcag gauacauuga cggaaaaaca acacaagaag acuuuuacaa guucuuaaag      360

aaagaacuaa augguauugc aggaucagaa agauucaugg aaaaaguaga ccaagaaaac      420

uuuuuacuua aacaacgaac aacagcaaau ggaguuauuc cacaucaagu acaucucaca      480

gaauugaaag caauaaucga aaggcaaaaa ccguauuauc cuucauugga agaagccaga      540

gacaaaauga uacgccuacu aaccuuuaga auccccuauu acguaggacc auuagcacaa      600

ggagaagaaa caagcucauu ugcaugguua gaacgaaaaa cuccagaaaa aguaacaccu      660

uggaaugcaa cagaaguaau ugacuacagu gcuucugcaa ugaaauucau acaaagaaug      720

auuaauuaug acacauauuu accaacugaa aaaguauuac cgaaacacuc aauacucuac      780

caaaaauaua caauauucaa cgaauugacc aaaguggcau auaaagacga aagagguauu      840

aaacaccaau uuucaucaaa agaaaaacga gaaauauuua aagaauuauu ucaaaaacaa      900

agaaaaguaa ccgucaaaaa gcuacaacaa uucuuaucag ccaauuauaa uauagaagau      960

gcagaaaucc ucggaguaga uaaagcauuu aacuccucau acgcaacuua ccacgacuuu     1020

cuugaucucg ccaaaccgaa caccgaaaga guagcugaac uucuggaaca accagaaaug     1080

aacgcaaugu ucgaagacau cguaaaaaua cucacaauuu ucgaagaucg agaaaugauc     1140

cgaacacaau uaaagaaaua ucaauccgua cuaggcgacg gauucuucaa aaagcuaguc     1200

aagaaacacu acacugguug gggaagauua ucagaacgac uaauaaaugg aauaagagau     1260

aagaaaacaa acaaaacaau auuagauuac cuaauagacg augacgacuu cccauauaac     1320

agaaaccgaa auuucaugca auuaauaaau gacgacucac ucagcuuuaa agaagaauua     1380

gccaaugaac ucgcgcuagc uggcaaccaa ucauugcucg aaguaguaga agcacuacua     1440

gguucaccag ccauuaagaa aggaauaugg caaacacuaa aaauaguaga agaacuaaua     1500

gaaauaaucg guuacaaucc caaaaauauc guggucgaaa uggcgagaga aaaccaaaga     1560

acaaauagaa guaaaccacg gcuaaaagca cuagaagaag cacucaaauc cuucgacuca     1620

ccuuuacuga aagaacaacc aguugacaac caagcacuac agaaagauag gcuauauuua     1680

uacuacuuac aaaacggaaa agauauguau acaggagaag cucuagacau agauagguua     1740

ucagaauacg auauagacca cauuauuccu cggucauuca uaguugacaa cucaauugau     1800

aacaaagucu uaguaagcuc aaaagaaaac cgacuaaaaa uggacgaugu uccagaucaa     1860

aaagugguaa uuagaaugag aagauauugg gaaaaacucc uacgagcuaa ccuaauauca     1920

gaacgaaaau uugcauaucu aacaaaacug gaauuaaccc cagaagacaa agcucgauuc     1980

auacaaagac aacucguaga aacaagacaa auaacuaaac acgucgcagc aauacuagau     2040

caauacuuua accaaccaga agaaaguaaa aacaaaggua ucagaaucau cacgcucaaa     2100

ucaucucuag uaucacaauu uagaaaaacu uucggcauaa acaaaguuag agaaaucaac     2160

aaccaucacc augcccauga cgcauaucua aacggaguag uagcgauagc auuacuaaag     2220

aaauauccca aacuggaacc agaguucgua uauggaaacu auacuaaauu uaaccucgcc     2280

accgaaaaca aagccacagc uaagaaagag uucuauucaa acaucuuacg auucuuugaa     2340

aaagaagaau acuccuauga ugaaaacggc gaaauauucu gggacaaagc aagacauaua     2400

ccccaaauaa agaaagucau cagcucacac caagucaaua uagugaagaa aguugaagua     2460

cagaccggcg guuucuacaa agaaacaguc aaccccaaag gaaaaccgga uaaacuaaua     2520

cagcguaaag cagguuggga cgucagcaaa uacggaggcu uuggaagccc agucguugcc     2580

uacgccguag cauuuauaua cgaaaaagga aaagcaagaa agaaagcaaa agcaauagaa     2640

ggaauaacaa uaaugaaaca aaguuuauuc gaacaagacc ccaucggauu ccuuaguaau     2700

aaagguuaca gcaacgucac aaaauucauc aaacugucaa aauacacauu auaugaacua     2760

gaaaauggaa ggcgucgaau gguggcuuca cauaaagaag cacaaaaagc uaacagcuuc     2820

auacuacccg aaaaauuagu cacuuuacuu uaccaugcac aacauuacga cgaaauagcc     2880

cacaaagaau ccuuugacua uguaaaugac cacuuaagcg aauuuaggga aauacuagac     2940

caaguaauag acuuuagcaa ccguuauacu aucgcagcga aaaacacuga gaaaaucgcc     3000

gaacuauucg aacaaaacca agaaucaaca guacaaucuc uaucccagag cuucauuaau     3060

uuaaugcaac uuaacgcgau gggagcaccc gccgacuuua aauuuuucga cguaauaaua     3120

ccccguaaaa gguauccaag ccuaacagaa aucugggaaa gcacaauaau auaucaauca     3180

auaaccgguc ugcgagaaac cagaacacgg auggcaacac ucugggacgg agaacaauga     3240


<210>  5022
<211>  3243
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5022
augaaaacua gcuucgccuu cgauauagga acgaacagca uugguuggug cguauuggcu       60

uuagacaaug caaaccugcc uuauagaaua uuagaugcug gcguaagaau uuuuagcgac      120

gggagggaac caaaaucagg aacauccuua gcugagggua gaagauuagc ucgcggaaug      180

aguagaagac gugauaggua caaacgaaga agaaaagcag ucauccguac acuaaucgaa      240

uauggacuaa ugccagauga uccagcugcu agaagggcau uaguagcgga aaccaacgac      300

aaagcuccag gcaaaaucgc aagcgacgug uaugcauuaa gagcuagagc uuuagaugau      360

aaauugccau uacaucauau ugguagagcu cuauuucaua uuaaccaacg aagaggauuc      420

aaaucaaaca gaaaaacuga uaaacgagau aacgaccaag guaaaauagc aucugguauc      480

ucccgacuua gagacaaacu agcaucagaa ggaaugcgua cacuaggaga auaucuaaau      540

cauagacauc aagcaggaaa uuggguuaga guaagaccau caagaguacc ugguaaagau      600

gauaaagaag aagacgguua cggauuuuau ccagaacgug cccuauugca agaugaguuc      660

aaugcuauau ggacagccca agcagcgcac uauccugacg uauuaacaga agaaagaaga      720

acacaucuag aaacaauaau uuucuuucaa agaccucuaa aaccagucca aguaggaaaa      780

ugcaguuuua aucccggaga agaaagacuc ccacgagcac aucccuuauu ccaagaauuu      840

cgacuuuaua aagaaguaaa ugaacuagag cuaguugaca gaaaucaaga ucaccacaaa      900

uuaucgcuag aucaaagaga cgcauuaaua gcacaauuga aaacacaaag agaagccgcc      960

uauucagcgc uaagaaaaac ggcgcgagca ccacuuggga cgcgcuucaa uaaagaaacu     1020

gaaucccgaa cgaaaaugaa aggagacgaa gucgccgcag aacuaucugc aaaaaccaga     1080

uucggacagg cuuggauauc gaugccucug gaagaaagau ggauaauugu agauaccuug     1140

agacgaguag aagacccacu agagcuaaca gcuuggcuag agcaacauua cggacuagac     1200

ggagaacaua uagaugcaau acuagcagcc cggcucccag aaggauaugg aagauuagga     1260

gaaacagcuu uaaccgaaau auuggucgag uuaaaggccg ccguaauaag cgaagcagaa     1320

gcugcuagga gagcaggaua ugaucaugcc uuauuacaaa gaggagcugg acuagaagca     1380

uuaccaaaau accaagaagu uauagcccgc aggaucccuc ccggaacagg gaauccggau     1440

gaucccuaug acguugaaaa aggccggauc acuaauccaa cuguucacau cggcuuaaac     1500

caacuacaaa aagucuuaaa ugcgcuuaua cgucgauaug gaaaaccuga acaaauugca     1560

aucgaacuag cacgugaauu aaaauuaaac gacgaacaga aaagagaaaa gaacauagaa     1620

auagccagaa auacuagagc agcggaacaa cgcucagcaa aauuaguaga acuuggcgug     1680

ccugauacag gauauaaucg ugugcuucua aaacuuuggg aagaacuaaa ccugcgagca     1740

ccagagaaca gaguuugcau cuacugcggu gaaccaauua auaucgaaac acuauucucu     1800

agcgcaguag auauagauca uauacuaccc uggucaaaaa cacuggauga uucucaagcc     1860

aauaggcuag uuugccacgu ggccuguaac aggcaaaagg cuaauuaugc gccagcagaa     1920

guaccacaau ggcaaggaag auacgacgac auauuagcaa gggcaaauac acuaccauug     1980

aauaaacgau ggagauuugg aagagacgcg augaaaaagu ucgcagacga agaaacauuu     2040

cuagcuagac aacuaaccga uacacaauau uuaaguagau uagcucaugc cuauauggcg     2100

gcucuuuacc cggaugaaga accugaugca caaggaguuc uagcaaggaa aaaucacguc     2160

agaguuauca cagggcgaau gacagaaaug uuacgaaggc aauggggucu caauucacua     2220

cuagcugauc auaaucagaa aaacaggaau gaccacagac accacgcaau ugaugcagca     2280

guaguugggg uaacaacacg agcucuuuua caaaggauag ccacaguggc agggggaaga     2340

gaagcacaag cacuugaaga aaccauagga aaaauugaac ccccauggcc cggcuuucgu     2400

gaugagcucc aagcuguaau aaaacgaauu guaguaucac auaaaccaga ucacgguacg     2460

uugccaaaac caggacaagg uggacaaaca gcuggucaac uacauaauga uacugcuuac     2520

gggcuaacag gcgaaaccga uucaagagga acgccuauag uaguaagacg aaaacccuuc     2580

acuucauuaa ccgaaaagga cuuaccauca auuagagaua gacaauuagc ccaggcacua     2640

gguucugcua uagcgggaag gucuggaaaa gaguugcaag cagcacuuga acaauucagg     2700

gauagagacg acuauaaaag caucagacau guacguguag uagaaccucu aaacgucaua     2760

ccaauaaggg auggaacagg cagagcuuau aaagcguaca aaggagaugc uaauuacaga     2820

uaugaugucu gggaacuacc aaauggaaaa uggaugcaug agguaguaag uauguucgac     2880

gcucaugaua gaucguggac aagugcagua cacauacauc acccagcggc acgaagagua     2940

uugucacuaa aacaaaauga caugguagca uaugaccauc cgcaagacgg accaacaaua     3000

gguagaguag uuaaauucuc acaaagcgga caaaugacau uugccccuca uaaagaagcu     3060

ggagcucuca aagcacgaga ugcugacccu gaagauagau uccgauauuu cuacaaauca     3120

gguucagcaa ugaaagauau aggacuacgu caaguaagag ucgacgaaau uggcagagua     3180

uuugaucccg gaccaagaga caaagaagcg agaaacgcaa gaaagaagaa aucgacagau     3240

uga                                                                   3243


<210>  5023
<211>  3243
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5023
auggcaaaau cuuucgcauu cgauaugggu accaacagua uaggaugggc aguauuaggu       60

cuagauagcg cgggagaucc agauucaaua gaaggugcag gcguccguau uuuuagcgac      120

ggaagagaac ccaagagugg agcuagcuua gcugaaggua ggcgaaucgc aagaggaaug      180

agcagacggc gagaaagaua uaaagcacga cgaaaggcac uaauaaggac acuuggugau      240

uauggauuaa ugccccuaga aagggcugcu agacgagcgu uaguggcaga gaccaacgac      300

agauccuuug gccuagucgc ccaggaugua uaugcgcuaa gagcaagagc cuuaguagaa      360

aaacuaccac ccuaucauuu ggggcgagca cuguuccacc ugaaucaaag acgagguuuu      420

aaaucaaauc ggaaaacaga cggacgagau aaugaacuag guaugauagc aacuggaaua      480

gcugcauuag acgcacaauu aaacggccgu acccuugggc aguaccuuca ugaaaugagg      540

ggugcugauc cuagacaaag ggggcaaguc agagucaggc cacaucuaga aauaaaugau      600

aaaggaagag aaauugcggc auacggauuu uauccucagc gcgcaauguu agaaagggaa      660

uuuaaugcaa uaugggcagc acaagcacca caucauccag aauuacucag cgaagaacgg      720

agagcacauu uauuucaagu caucuuuuac caaagacccc ugaagcaacc agauccagga      780

agguguuccu uuaauccagc agaaccccgc cuggccaaag cccauccucu auuucaaagg      840

uuccgccuag uaaaagagau aaaugaacug agaauuguaa cagguagcaa uagucaggga      900

guaccauuaa cucuagaaca acgcgauauc cuacuaacgc uacuaagaga uaagaaagca      960

ucuaguuuua aaacauuauu agcaaaaaua auaaaaggac aaccagaagc cagauucaac     1020

aaggcuucug acagcagaga uaaaaugaaa ggggaugaac uagcagcgac uaugagugac     1080

aaaacgagau ucggacaagc augguuugca augccugaag cagaacaaug gaggaucauu     1140

gcuaaauuaa gagauauaga cgaccccuua gaauuaaaug caugguuaga aagcgaauau     1200

ggacuagcuc cggagagaag ggaugcagua gcaaaugcau uacuaccuca aggauaugga     1260

cgacuaggag aaacagccuu aagaacaaug uuagauuauc uugaagccgc aguaauuagu     1320

gaggcagaag cugcagcuca ggccggcuac gaucaugccc uagcuagaag aggagcaggc     1380

uuagaucaac uacccaaaua ucaagaagua uuaggacgcc guauaccucc aggaaguggc     1440

gagccaaacg auccguacga cauaaggaug ggacgcauuu cuaacccaac uguucacaua     1500

gcacuaaacc aauuaagaag gguaguaaau gcacuaauac gcaaguacgg uaagccagca     1560

gaaauugcua uagaacuagc aagagauuua aaacuaucag aggaacaaaa gagagaagua     1620

aaugcaacua ucgcaaagaa caccagagau gcggaaagac gagcaguaag auuagcagau     1680

gaagguauug auaauacagg gcacaacaga gcuuuauuac guuucuggga ggaguuagcg     1740

gcggacccac uagaucgagu uuguguauau ugcggugggg guauaggagc ggcaagucua     1800

uuuaacggaa guguagacau cgaucacaua uuaccuuggu cuagaaccuu agacgauagc     1860

agggcaaaua gaauauuggu ucacaacagc uguaacaggc uuaaaagaaa cuuugcgcca     1920

uuugaaguac cggaauggcg agaaauauau caagacauau uaguaagagc agcuaaacua     1980

ccagcaaaua aaaguuggag auuugcaccu gacgcaaugg aucgauucga aggagagaaa     2040

gauuuccuag ccagacaauu aacagauaca caguaucugg caagaauggc ucacgaauau     2100

uuagcagccu uauauccgga ugaggaaccu gaugaauggg gaguauucgc acgcggaaau     2160

cauguaagag ugguaccagg gagacuaaca gaaaugcuca ggagagcaug ggguuuaaau     2220

gcaauccuua gugaugauaa cagaaagaau agaaaugacc accgacauca cgccauagau     2280

gcagucguug uaggagucac cacgagagac auguugaaca aaauaucaca agcagcagga     2340

agaggagcag auaugggugc agcagaugcg uugcgaagau uugauccucc auggccauca     2400

uuccaccaag ccguaagagc agccgucgaa agaauauuag ucagccaucg agcagaucau     2460

gggaccuuac ccggaacuga cggacaagga aguacagcag gucaacuuca uaaugauaca     2520

gcauaugguu uaacagggga aaccagaaaa ggauuagcgg uuguaguucg uagaaaacca     2580

uauuuaucuu uagaaccgcg ugauauagaa acuaucagag acccacaauu aagagaucau     2640

cuagccacag aaaccuaugg gcucgcggga gccgaguuca cggcagcgcu aaaaagauua     2700

caacaaacag acaaauuuua ugcaggaaua cgacgaguua gaguaacgga agcauuaagu     2760

guaauaccga uaagagaugc uacgggacac gcguauaaag cauacaaagg agaugcuaau     2820

uuucguuaug auguguggga auuacccaau ggaaaauggg uuccugaagu uauaaguaug     2880

uuugaugcgc augauagagg augggugucc caaguacaug cugcaaaucc agccgcacga     2940

cgugucuuaa gccucagaca aaaugacaug guugcuuacc agcacccgga acaaggccua     3000

guauacggaa gaguaguaaa auucgggcaa ucaggccaaa ugacuuucgc agcacaccac     3060

gaagggggau cuuuaaaaag ucgcgacgcc gauagagaug auccauuuaa auauuucuac     3120

aagucugcag gaacaaugaa agauauagga uuaagacaga uaagaguaga ugaaauaggu     3180

agaguacaag auccaggacc ucaagaucga gauucaaaaa gaguacgaaa aagcgacagg     3240

uga                                                                   3243


<210>  5024
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5024
auggcugcgu uuaaacccaa ucccaucaac uauauauuag gacuagauau agguauagca       60

ucuguaggau gggccauggu agaaauagac gaagaugaaa acccuaucug ucuuauagac      120

cuaggaguac guguauuuga aagagcagaa guaccaaaaa cuggcgauag ucuggcuaug      180

gcaagacgau uagccagauc aguaagaaga uuaaccagga gacgagcaca ucgacuacua      240

cgugcuagga gguuacucaa aagagaaggc gugcuacaag cagccgauuu ugaugaaaau      300

ggcuuaauaa aaaguuuacc aaauacccca uggcaacucc gagcagcagc gcuugauagg      360

aaauuaacac cccuggaaug gagcgcagug cuauuacauc ucauaaaaca ucguggauac      420

cucagccaac guaaaaacga aggggaaacu gccgacaaag aauuaggugc auuacugaaa      480

gggguagcag auaaugcaca cgcccugcaa acaggcgauu ucagaacacc cgcuaaauua      540

gcacuaaaca aauuugaaaa agaaucaggu cacauacgaa accaaagagg agauuacagc      600

cauacuuucu cacggaaaga cuuacaagcu gaacugaacu uacuauuuga aaaacaaaaa      660

gaauuuggca acccucacau aagcgauggc cugaaagaag gaaucgaaac gcuucuuaug      720

acacagagac cagcauuaag cggagaugcu guacaaaaga ugcucgguca cugcacauuu      780

gagccaacag aacccaaagc agcuaaaaac acauacacug cagaaagguu uguauggcuu      840

acaaaacuca acaauuuacg gauauuagaa caagggucag aacaaccauu aacagauaca      900

caaagggcaa aauugaugga cgaacccuac cguaaaucaa aauuaacaua ugcccaagca      960

agaaaacuac ucggacucga agauacggca uucuucaaag gacuacgcua uggaaaagau     1020

aaugcagagg cuucuacucu aauggaaaug aaagcguauc augcaauuag cagagcucua     1080

gaaaaagaag gguuaaaaga uaagaaaagu ccccucaauu uaucaccuga auuacaagau     1140

gaaauaggua cagccuuuag uuuguucaaa acagaugaag acauaacugg aagacuaaaa     1200

gaccgaguac aaccagaaau auuagaagca uuauuaaaac auauuucuuu cgacaaauuc     1260

guacaaaucu cuuuaaaagc acuaagaaga auaguaccac uaauggaaca agguaaacgu     1320

uacaacgaag ccugcgcaga gaucuaugga gaucauuacg ggaagaaaaa uacggaagag     1380

aaaauauauu uaccuccaau accagcugau gaaauaagaa aucccguagu cuuaagggca     1440

uuaagucaag caagaaaagu aauaaaugga guagugaggc gauacgggag uccggcgagg     1500

auucauaucg aaacagcgag agaaguaggu aaaaguuuua aagaucggaa agaaauagaa     1560

aaacgacaag aagaaaacag aaaagacaga gagaaagcag cagcaaaauu ucaagaguau     1620

uuucccaauu ucguaggcga accaaaauca aaagacauuu uaaaacuuag acuauaugaa     1680

caacaacaug ggaaaugucu auauucggga aaagaaauaa auuuaggaag auuaaaugaa     1740

aaaggcuaug uagaaauaga ccaugcauua ccauuuucca gaacauggga ugacucguuc     1800

aauaauaaag uucuuguucu ggguucagaa aaucaaaaca aaggaaauca aacaccguau     1860

gaauauuuua acggaaaaga uaacagucgu gaauggcaag aguuuaaagc uaggguugaa     1920

accagcagau uuccaaggag uaaaaagcaa aggauuuuau uacaaaaauu ugaugaggau     1980

ggauuuaaag aaagaaaucu uaaugacacg agguauguaa accgauucuu augucaauuu     2040

guagcagauc gaaugagacu aacuggaaaa gguaagaaac gaguguuugc auccaaugga     2100

caaauuacaa acuugcuaag aggcuucugg gguuuaagaa aaguuagagc ugagaacgac     2160

aggcaccaug cucuagacgc agucguagua gcauguucca cagucgcuau gcaacagaaa     2220

auaacccguu uuguucggua caaagaaaug aacgcauucg auggaaaaac aauagauaaa     2280

gaaaccggag aaguacuuca ccaaaagacu cauuucccac aaccauggga auuuuucgca     2340

caagaaguga ugauaagggu uuucggaaaa ccagauggaa aaccugaguu cgaagaagcu     2400

gauacacccg aaaagcuaag aacguuauua gcggaaaaau uaucuagcag accugaagca     2460

guccacgaau auguuacucc ucuauucguc ucuagagcac caaauaggaa aaugagugga     2520

caaggacaca uggaaacggu aaaaagugcu aaacgauuag acgaaggagu gucaguacua     2580

agggugccau ugacacaauu aaaauuaaaa gauuuagaga aaaugguaaa uagggaaagg     2640

gaaccaaaau uauacgaagc acuaaaagca cggcuagaag cacauaagga ugauccagca     2700

aaagcguucg ccgaacccuu cuacaaauau gauaaagcag gaaacagaac acaacaaguu     2760

aaagcggugc gaguagaaca aguucagaaa acaggaguau ggguuagaaa ucauaacgga     2820

auagccgaua acgcaacaau gguaagaguu gauguuuucg aaaaaggaga uaaauauuac     2880

cuaguuccaa uauauucaug gcaaguugca aaaggcauac uaccagacag ggcaguugua     2940

gccuacgcag acgaagaaga uuggacagua auagaugaau cauuuagauu caaauuugua     3000

cuauacucua augaucuaau aaaaguucaa cuaaagaagg acagcuuuuu aggauauuuc     3060

ucagguuuag acagagccac aggggcaaua ucauuaagag aacacgacuu agaaaaaucg     3120

aaaggaaaag auggaaugca uagaauuggu guaaaaacag cuuuaucauu ucaaaaguau     3180

caaauagaug aaauggguaa ggaaauccga ccaugucgau uaaagaaacg accacccguu     3240

cgcuga                                                                3246


<210>  5025
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5025
auggcggcau uuaaaccaaa cccaauaaau uauauccuag gacuagauau aggaauagca       60

ucaguaggau gggcaauggu agaaauagau gaagacgaaa auccgauaug ucugauagau      120

uuaggaguca gaguuuuuga aagagcagaa guaccaaaaa caggugauuc ucuagcugca      180

gcccgaagac uggcucguuc aguaagaaga cuuacaagga gaagggcuca uagguugcuc      240

agagcuagaa gguuacuaaa acgagaagga gucuuacaag cugcagauuu ugaugaaaau      300

ggacuaauaa agagcuuacc aaauacccca uggcaauuac gaucggcagc acuagauaga      360

aaauuaaccc cuuuagaaug gucagcugua cuacuacacc uaauuaaaca ucgaggauau      420

cuaagucaaa ggaaaaauga aggugaaacu gcagauaaag aacuuggcgc auuauuaaaa      480

ggcguugcag auaaugcuca cgcacuccaa acuggagauu uucgaacacc agcggaauug      540

gcucuaaaca aauuugaaaa agaaucagga cauauuagaa auagacgcgg agauuacuca      600

cacaccuuuu ccagaaaaga ccuacaagca gaacuugacc uauuauucga aaagcagaaa      660

gaguucggua auccacauau aagcgacgau cuaaaagaag gaauagaaac uuuguuaaug      720

acgcaacgac cagccuuauc uggugaugca guacagaaaa uguuagguca cuguacauuu      780

gaaccaacag aaccaaaagc agcuaaaaau acuuacaccg cagaaagauu uguaugguua      840

acuaagcuaa auaaccuaag gauauuagaa caaggaagcg aaagaccauu aaccgacaca      900

gaaagagcaa cccucaugga cgaacccuau cguaaaucaa aacugacaua ugcacaagca      960

agaaaacuuu uagaacuaga cgauacugca uucuuuaaag gauuaagaua uggaaaagac     1020

aaugcagaag caaguacguu aauggaaaug aaagcauacc acgcaauaag uagagcuuua     1080

gaaaaagaag gauugaaaga uaagaaaagc ccacuaaacc uaaguccaga auuacaagau     1140

gaaauaggaa cggcguuuuc acuauuuaaa accgaugaag auauuacagg gcgucuaaaa     1200

gacaggguac agccagaaau cuuagaagua uuauuaaaac acauuaguuu ugauaaauuu     1260

guacaaauau cacugaaagc auugagaagg auagucccau uaauggaaca aggaaaaaga     1320

uaugacgaag caugcgcaga aauuuacgga gaucacgacg gaaagaaaaa uacagaagag     1380

aaaauauacc uaccaccgau ucccgcugau gaaauaagaa acccaguagu auuaagagca     1440

uugucacaag cacgaaaagu uauaaacgca guaguaagaa gauaugguuc accagcaaga     1500

auacacauag aaacagcuag ggaaguggga aaaaguuuua aagauagaaa agaaauagag     1560

aaaagacaag aagaaaauag aaaagauagg gaaaaagcag cagcgaaauu ccgagaauac     1620

uuuccuaacu uuguaggaga accaaagucc aaggacauuc uaaaauuaag acuguacgaa     1680

caacaacaug gaaaaugucu auacucagga aaagaaauaa acuuaggaag auuaaaugaa     1740

aaaggcuaug uagaaauaga ucacgcauua ccauucaguc gaacuuggga cgacuccuuc     1800

aacaauaaag ugcugguccu cggaagugaa aaucaaaaca aagguaauca aacuccauau     1860

gaauauuuua acggaaaaga uaacucucga gaauggcaag aguucaaagc acgaguagaa     1920

acuagccgau uccccagauc aaagaaacaa cguauauuau uacaaaaauu ugaugaagaa     1980

ggauuuaaag aacgaaauuu aaacgauacu agguauguca auagauucuu gugucaguuc     2040

guagcagauc auauguuguu aacugguaaa gguaaaagac guguauucgc cucuaacggc     2100

caaauaacaa acuuguuaag gggauuuugg ggauuaagaa agguuagagc cgaaaacaac     2160

cgccaucaug cacuagacgc cgugguagua gcaugcucaa caguugcaau gcaacagaaa     2220

auaacacgcu ucguaaggua uaaagaaaug aacgcguucg augggaaaac aauugauaaa     2280

gaaacgggag aaguacuaca ccagaaaacu cauuuuccac aaccauggga auuuuucgcu     2340

caagaaguga ugauuagagu auuugguaaa cccgacggga aaccugaguu cgaagaagca     2400

gacacccccg aaaaauuaag aacgcuauua gcggaaaaau ugucaucgag accagaagca     2460

guacaugagu auguaacacc uuuauuuguc uccagggcac caaaccgaaa aaugaguggu     2520

caaggacaua uggaaacggu aaagagcgca aaacgacuag acgaaggugu gucaguccuc     2580

cgagucccac ugacacaauu aaaauuaaaa gacuuagaga aaaugguuaa uagagaaagg     2640

gaaccaaagu uauaugaagc acuaaaggca cguuuagaag cacauaaaga ugauccagca     2700

aaagccuucg cagaacccuu cuauaaauau gacaaagccg ggaauaggac acaacaggua     2760

aaagcuguaa gaguagaaca aguccagaaa acuggcguau gggugcgaaa ucacaaugga     2820

aucgcagaua augcaacgau gguccguguu gauguuuuug aaaaagcugg aaaauauuac     2880

uuaguuccaa uauacucgug gcaaguagcg aaaggcauuc uaccagaccg agcuguugua     2940

gcuuacgcgg acgaagaggg guggacagua auagaugaga gcuuuagauu caaguucgug     3000

uuauauagua augauuuaau aaaaguccaa cuaaagaaag acaguuuucu agguuauuuu     3060

ucaggucuag aucgagccac aggcgcaauu agucuucgag aacacgacuu agagaaaagu     3120

aaagggaaag auggcaugca cagaauuggu gucaaaacag cacuaucauu ucaaaaauau     3180

caaauagaug aaaugggaaa agaaauacgu cuaugccgau uaaagaaaag acccccagua     3240

agauga                                                                3246


<210>  5026
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5026
augaccgcau ucaaaccuaa ucccauuaau uacauacugg gcuuagacau agguauagcc       60

ucaguagguu gggcaauggu ugaaauugau gaagaagaaa acccgauuag gcuuauagac      120

uuaggaguaa gaguauucga acgagccgaa gucccaaaaa caggagacag ucuagccgca      180

gccagacgac uagcaagaag cgucagaagg uugacaagac gcagagcuca cagacuauua      240

agagccagga gauuguuaaa aagggaaggc guucuccaag cagcugacuu cgacgaaaau      300

gguuuaauua aaucucuucc uaauacaccu uggcaacuaa gagccgcugc auuggauaga      360

aaacucacuc cucuugaaug gagugcuguc cuguuacauc uaauaaaaca ucgagguuac      420

uuaagccaaa gaaaaaauga aggagaaacg gcagauaaag aacuaggcgc gcuucuuaaa      480

ggaguagcag auaaugccca ugcgcuacaa acaggagauu ucagaacgcc ugccgaacua      540

gcagucaaca aauuugaaaa agaaucuggc cauauuagaa accaaagagg cgauuacucc      600

cacacauuuu cgcgaaaaga uuuacaagca gaauuaguau uauuauuuga aaaacaaaaa      660

gaauuuggca acccacauau aucugacggc cugaaagaag gaauagaaac acucuuaaug      720

gcacaacgac cagcacuaag cggagaugcg guccaaaaaa uguuagguua cuguaccuuc      780

gaacccacag aacccaaagc ggcaaaaaau accuauacug ccgaaagauu uauaugguug      840

acgaagcuaa auaaccuacg aauccuagaa caagguucag aaagaccacu uacagauaca      900

gaacgagcaa cauuaauaga uaugccauac aaaaauaaau uaacuuacgc aaaagcacgu      960

gaacuacuag guuuagauga cacagcuuuu uucaaagguc uucgguaugg caaagauaac     1020

gcagaaagcg cuacccucau ggaaaugaaa gcauaucacg caauaagacg ugcacuagaa     1080

aaagaagguc uaaaagauaa aaaaagucca uuaucucuau caccagaacu acaagaugag     1140

aucggaacag cauuuagcuu auuuaaaacg gacgaagaca uaacgggaag auuaaaagga     1200

uguuuacaac cagagauucu agacgcauua cuuaaacaua uaaauuuuga caaauucguu     1260

caaauuucac uaaaagcauu aagacgaaua guaccauuaa uggaacaggg aaaaagguau     1320

gacgaagcau gcgcugaaac cuauggggac cacuacggug aaaaaaauuc agaagagaug     1380

cuauacuuac cacccauacc agcagaugaa aucagaaacc caguagucuu acgagcauua     1440

ucucaagcaa gaaaaguaau uaauggagug guacgaagau acggaucacc agcaagaauu     1500

cauauugaaa cagcacgcga aguagguaaa aguuucaaag auagaaaaga aauagaaaaa     1560

agacaagaag aaaauagaaa agauagggaa aaagcagcgg caaaauuuag ggaauauuuc     1620

ccgaauuucg ucggugaacc aaaaucaaaa gauauauuaa aacuuagauu auacgagcaa     1680

caacacggca aauguuuaua cucaggcaaa gcaaucgaua uuagacgauu aaacggaaaa     1740

ggauauguag aaauagauca ugcccuuccu uuuucuagaa cgugggauga uucuuuuaau     1800

aauaaaguac ucguucuagg aucugaaaac caaaacaaag gaaacagaac accauacgaa     1860

uaucuuaacg gaaaagacaa cucaagagaa ugguuagaau uuaaagcaag aguugaaacu     1920

agcagauuuc cucauaguaa aaaacaaaga guuuuacuac aaaaauucga cgaaaaaggu     1980

uuuaaagaaa ggaaucucaa ugacaccaga uacgugaaca gguuuuuaug ccaauuugua     2040

gcugaccaua ugcaacuaac agguaaaggg aaacgaagag uguucgcuag uaacggacaa     2100

auaacaaauc uauuaagagg cuucugggga cugcauaagg uaagagcgga aaacgacaga     2160

caucaugcuu uagacgcagu aguaguagca ugcucuacag uagccaugca acaaaaaaua     2220

acauguuuug ugagauauaa agaaaugaac gcauucgaug gaaaaacugu agacaaggaa     2280

acaggugaag uauugcauca aaagacccac uucccacaac cuugggaauu cuucgcacaa     2340

gaagucauga uaagagucuu uggaaaacca gauggaaaac cggaauucga agaagcagau     2400

acacccgaaa aacuuagagc gcuacuagca gaaaaacuau caagcagacc agaggcugua     2460

caugaauacg uuacgccacu auucguauca agagcaccaa auagaaaaau gucuggagcu     2520

cauaaagaua cgcuacguuc agcaaaaagg uucguaaagc auaaugaaaa aauaagugua     2580

aaacgggucu ggcuuacaga aauaaaauua gcagaucuag aaaauauggu aaauuacaaa     2640

aauggacgag agauagaauu auaugaagcc cucaaagcaa ggcuaggggc auacggagga     2700

aaugccaaac aagccuucga uccgaaagau aacccuuucu acaaaaaagg aggccaacua     2760

guuaaagcgg uaagaguaga aaaaacacaa gaaucuggcg uauuacuaaa uaagaaaaac     2820

gcauacacaa uugcagacaa cggagacaug guaagagucg acgucuuuug caaaguagau     2880

aaaaaaggaa aaaaucaaua uuuuauugua ccgauauaug cauggcaagu agcagaaaac     2940

aucuuaccug acauugauug caaagguuau cgaauagaug auaguuauac auuuuguuuc     3000

agucugcaua aauaugaucu aauagcuuuu cagaaagaug aaaaaucuaa aguagaauuu     3060

gcguauuaua uaaauuguga uuccucaaac ggacguuucu auuuagcaug gcaugacaaa     3120

ggaucaaaag aacaacaauu cagaauaagc acacaaaauc uaguauuaau gcaaaaauac     3180

caaauagaug aguuaggaaa agaaaucaga ccaugucgac ucaaaaaaag accaccgguc     3240

agauga                                                                3246


<210>  5027
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5027
auggcggcgu ucaaaccaaa ccccauaaac uacauauuag gacucgauau cggcauugca       60

agcgucggau gggcaauggu cgaaauagau gaagacgaaa auccaauaug ucuuaucgau      120

cuuggaguac guguauuuga acgugcugaa guaccuaaaa ccggagacag uuuggcagcg      180

gcucggcguu uagcuagguc gguaagaaga cuaacacgaa gaagggcaca ucgucuacua      240

agggcaagaa gacuacucaa aagagaaggc guacuucaag cagcugauuu cgacgaaaac      300

ggauuaauaa aaucucuccc aaacacacca uggcaauuaa ggagugcggc uuuagauaga      360

aaauuaaccc cacuggaaug gagugcugua cucuuacacc ugauaaaaca uagaggauau      420

cuuagccaac gcaaaaauga aggugagaca gcugacaagg aauugggcgc cuuguuaaaa      480

gggguagcua acaacgcgca cgcauuacaa acaggcgauu ucaggacucc agcugaauua      540

gcguuaaaca aauucgaaaa agaaagugga cauauaagga aucaaagggg cgauuauucu      600

cacacuuuua guagaaaaga cuuacaagcc gagcuaaucu uauuauucga aaaacaaaaa      660

gaguucggaa acccccaugu uuccgggggu cuaaaagaag gaauagaaac uuuacuaaug      720

acacaaagac cugcacuuuc gggcgaugca guucaaaaga ugcucggaca cugcacauuu      780

gaaccagcag aaccaaaagc ugcuaaaaau acauacaccg ccgaaagauu uauauggcuc      840

acuaaauuaa auaacuuacg cauuuuagaa caaggaagug aaaggccacu aacagauaca      900

gaacgcgcaa ccuuaaugga ugaaccauau cgcaaaucca aacuaacaua cgcacaggcu      960

cgcaaauuac uaggccuaga agauacagcu uucuuuaaag gacugaggua cggaaaagac     1020

aaugcagagg cuucaacucu uauggaaaug aaagcauauc acgcgaucuc aagagcucuc     1080

gaaaaagaag gacuuaaaga uaagaaaagu cccuuaaauu uaucaccaga acuacaagau     1140

gaaauuggaa cagccuuuuc auuauucaaa accgaugaag auauaacagg aagauuaaaa     1200

gauagaauac aaccagaaau ucucgaagcc uuauuaaaac auaucuccuu cgauaaauuu     1260

guucaaauau cgcuaaaagc acuucgacga auaguaccac uaauggaaca ggggaaaaga     1320

uaugaugagg cuugcgcaga aauauaugga gaucauuacg gaaagaaaaa caccgaagag     1380

aaaauauacu uaccuccaau cccagcugau gaaauccgaa accccgucgu acugagagcu     1440

uuaagucaag caagaaaggu aauuaauggc guaguaagaa gauauggaag ccccgcaaga     1500

auacauauag aaacagcgag agaagucgga aaaaguuuca aagacagaaa agaaauugaa     1560

aagaggcaag aagaaaauag aaaggacagg gaaaaagcag cagccaaauu uagagaauau     1620

uuuccaaauu uuguaggaga accaaaaagc aaggacaucu uaaaacuacg auuguaugaa     1680

caacaacaug gcaaauguuu auauucaggc aaagaaauaa accuagguag auuaaacgaa     1740

aaaggguaug uagagauaga ucaugcauua cccuucucca ggacauggga ugauaguuuc     1800

aauaauaaag uauuaguacu aggaagcgaa aaucagaaca aagguaauca aacaccuuau     1860

gaauacuuca acgguaaaga caacucacga gaauggcaag aauuuaaagc acguguagaa     1920

acuagcagau uuccgcgauc uaagaaacaa agaauauuau ugcaaaaauu cgacgaagac     1980

ggauuuaaag aaagaaaucu aaacgauaca agguauguaa accgauuccu augucaauuc     2040

guugcagaua gaaugagacu uacaggcaaa ggaaagaaac gaguguuugc aucuaaugga     2100

caaaucacaa aucucuuaag aggauuuugg ggacuaagga aaguacgcgc agagaaugau     2160

agacaccaug cauuagacgc aguaguaguc gcaugcucca caguagcaau gcaacagaaa     2220

auaacaagau ucguuagaua caaagaaaug aaugccuuug augguaaaac aauugauaaa     2280

gaaacgggag aaguauuaca ccagaaaacg cacuuuccac aacccuggga guucuucgcc     2340

caagaaguaa ugauacgagu cuucggaaaa ccugauggaa aacccgaauu ugaagaagcu     2400

gauacaccag aaaaauuaag aacacuguua gcugaaaaau uaucaagcag accagaagca     2460

guacacgaau augucacucc ccucuucguc aguagagcgc cuaacagaaa aaugagugga     2520

caaggacaca uggaaacugu uaaaagugcc aaacgucuag augaaggagu aucaguuuua     2580

agaguaccac uaacacaacu caaacuaaaa gaucucgaga aaaugguaaa cagagaaaga     2640

gaaccuaaac uuuaugaggc acucaaagcc agacucgaag cacauaaaga cgauccagca     2700

aaagcauucg cagaaccauu cuauaaauau gauaaagcug guaacaggac ccaacaagug     2760

aaagccguac gaguugaaca aguccagaaa acaggaguuu ggguaagaaa ccauaaugga     2820

auagcugaca augcaacgau ggucagagua gacgucuuug agaaagcagg aaaguauuac     2880

cuaguaccaa uauauuccug gcaaguagcu aaaggaauau uaccagauag agccguagua     2940

gcauaugcag augaggaagg uuggacaguc auagacgaau cauuuagauu caaauuugug     3000

uuauacagua augauuuaau caaaguacaa cucaagaaag auucauuccu cgguuauuuc     3060

ucagggcuag auagagcgac aggggcaauc ucacuaaggg aacaugauuu agaaaaauca     3120

aaaggaaaag acggcaugca uagaaucgga guuaagacug cacuauccuu ccaaaaauac     3180

caaaucgacg aaaugggaaa agaaauacgu uuaugcagau uaaagaaaag gccacccgua     3240

cguuga                                                                3246


<210>  5028
<211>  3246
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5028
augcuaaaaa gaguuuuagg aauagauguc ggaauaucca gcauaggaug ggcacuccuc       60

gaauuucaag gagacauaga caacaaagau cuaccuuccg gaaaaaucuu ggcaucaggg      120

guaagaguuu uuacucaagc agaaaacccg aaaaacggcg agucacuugc gaaaccacga      180

cgagaugcaa gauuggcacg aagaagaacc agaagacgag cacagaggcu aacagauaua      240

aaagauuuau ucuucaaaua uaaacucguc accgggauaa gcagaaauaa aauuaauagu      300

auauacaaaa ccgaagaaaa uaaaaaagac ccuuggacuu uaagaaaaga agcuuuauac      360

agaaaacuag acggagaaga guuugcaaga guguuaacuc acauagcaaa acacagaggg      420

uucaagucua ugagaaaaag cgaagaagaa ggcagcgaca caaaagcuaa agaaacgcaa      480

agaguuuuaa cagaaauagg aaaaaacaaa gacacacuaa agacaggcau auggcaaaca      540

gccggagaga ugauugcaag caggccaaaa ggagacgcca gacgaaacaa aacugacuca      600

uauaaaaaua gcauaucgag ggagcuacua caagaugaga caaagauaau auucgaaaaa      660

caaagaggaa ugaauucaca auucacguca gcagaucuag aaaaagaaua cagcgauaua      720

gccuuccacc aagcaccaau acaaucaaug cucaaaaugg ucgggggaug uacuuuugaa      780

aaggaugaag aagguaaacg auuaaaacga gccccaaaaa acucauauuc cgcagaaguu      840

uucaccgcuu uaacuaaaau aaauaaccua guacuuaaaa cuaaaacugc caacaucaac      900

gaccgcuucc uuacuacaga ggaaagaaaa aaggcacuag aacugugcca uucaaaugca      960

aaaguaacau ucaaacagcu uagaggugaa uuagggcuac cagaagacug gaucuucaac     1020

acaguagacu augcaaagaa accgaaaaaa accgccguca aagaaaccga acccgaaucu     1080

gcuugcuucu uccagaugag aggauauaau gaaauaagaa aaagcguggg aaaggacuug     1140

uggaccgaaa uaagcaugaa aaaagaaaua cuaaauagua uaguuacaac acuagcaaca     1200

gaaaaaagug acgaagccau caaggcucaa uuaaaaaaaa gugaaguccc ggaugaaauu     1260

gccgaaaaag uacuaggacu uaacuucaga gguuuucugc accuaaguau aaaagcucug     1320

gacaagauaa uuccguucau gauggcaggu gacaaauaca cagacgcaug ugauaaagca     1380

gguuacaagg aacaaauuag uggcauaaag aaaauauuuc ucagaaaacu aaacgaugaa     1440

gaaaacgaac aaauaacaaa ccccguagcc aagagagcua ucacacaguu uagaaaagua     1500

auuaacgcga uaauaagaga auacgggaaa uuugaccaaa ugaauauaga auuagcuaga     1560

gaucuagcaa aaucauacga cgacaggaaa aaaaucaaag aaggacaaga agauuucaga     1620

gcugaaaaag accgagccgc ccaaaggugu aaagauaaug gcuuagaucc aaauaguaaa     1680

gacaacuuaa guaaauaccg gcuaugggaa cagcaggaca acggauucug uauuuacugu     1740

ggcaaaccaa uagaacuaca gagacuggcu gaacaagguu acugugaagu agaucauaua     1800

cuacccuaca gccgaagcuu agaugacuca caacacaaca aaguacuagc ccacauacau     1860

gauaaucaac aaaaaaaaaa ucaaaucccu uuugaacacu uuggaggaga uggaccaaaa     1920

ugggaagaau ucguaggaag acuaaacagu caauucaaaa acuuaccaaa aucaaaacua     1980

aauagauuag ugaaaagaaa auucgacgaa aaagacaugg aaggcuucaa agaaagaaac     2040

aucaaugaca cacgauauau aucaagauuc auaaaaaauu uugucgagaa ccacuuagaa     2100

uucgcugaaa gcgcacaaaa acaaaaagua uucguacgaa gcgggucuuu aacagcauuu     2160

cugagacauc aaugggguuu gauaaaaaaa agagacgaau cagaucgcca ccacgcacua     2220

gaugcacucg uaacugcaug ugcaacacaa gccaugguaa aaucuacugc aauaacagcu     2280

caaaaaaugg aaaguuauaa uuggauaaau aaagaaaaac cuagaaugcc acuaccaugg     2340

cugaacuuuc gagacgacgu acaaaaaagc cuggaugccg uauucguaag ccgagcucca     2400

cgacacaaag uaggaggcgc ugcacacaaa gaaaccauaa gauccauaag aaaaugcggc     2460

gaagaaaauc uaauaaucga aaaaaccccc uuaguagaua uugcaacgaa aucaggaauc     2520

aaacucaaag acauugaaaa acuauauaaa aaagaacaga auagaaaacu cuacgaaaca     2580

uuaaaggaaa gauuagagca aaauggaaac gacccuguua aagcauuugg aaaccccgau     2640

gacccaguuc guaugccaac caaugaucua acaaaaacgg gaccaauuau aaaaucaaua     2700

aaacuaauaa cgaauaaaaa aagcggauua aacguaagag caggaauagc ugacaauggc     2760

gaaauagcaa ggcuagacgu uuacgaaaga aaaggaaagu ucuaccucau accgaauuau     2820

guagcugauu ucgcaaacaa agaauuaccu aaaaaggcca ucaaagcgaa uacaccagaa     2880

acagaauggc caauaauaga cagaaacuac aauuuuaaau ucucgcuguu caaagaugau     2940

cugauaagua uaaaacaaaa auuagacagu ccagaagagu ucuauuauua uaaaggaccc     3000

gacgugucaa cggcaucuau aacuguagaa agcgucgaua gaaaacggcu augggaagaa     3060

aaaaaaaaag gaaaaauuga aaacgucuuu cauacccgaa ucgggacaaa gacacuaaaa     3120

aguuuuagaa aauuuaacuu agauauauua ggcacuaaag caccaguaga aaucaaaaaa     3180

gaaacauggg uuggaguaau acggaaaaac ggagucgcaa acaacacagg uaacaaagca     3240

auguga                                                                3246


<210>  5029
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5029
auggccgccu uuaaaccaaa uccgaugaac uauauauuag gacuugauau aggaauagcg       60

aguguaggau gggcuauggu ugaaauagac aaagaagaaa acccaauaag auuaauagau      120

cuaggagugc gcguuuuuga aagggcggaa guaccaaaaa caggagauuc acuugcaaug      180

gccaggagac uugcaagaag uguaagaaga cuaacuagaa ggcgagcuca uagacuacuu      240

agggcaagaa gauuacuuaa aagagaaggg guauuacaag augcagauuu ugacgaaaac      300

ggacucguga aaagucuacc gaauacucca uggcaauuaa gagcugccgc auuagaucga      360

aaacucacuu guuuagaaug gagugccgua cuacuacauc uaauaaaaca cagaggcuac      420

uuaagucaaa ggaaaaauga aggagaaacu gcagauaaag aauuaggugc gcuauugaaa      480

ggcgucgcug auaaugcaca ugcccugcaa acuggagacu uuagaacacc cgcagaacua      540

gcacuuaaca aauucgaaaa agagaguggu cauaucagaa accaaagagg agacuauuca      600

cacacuuucu caagaaaaga cuuacaagca gaacuaaauu uauuauucga aaaacaaaaa      660

gaauuuagua auccacaugu aucagauuca cuaaaagaag gaauagaaac acuauuaaug      720

gcacaacgcc cagcguuaag cggggaugcg guacagaaaa ugcuagguca uugcacauuu      780

gaaccagcag agccaaaggc agccaaaaau acauauacug cagaaagauu cauaugguua      840

acuaaacuaa auaacuuaag aauuuuggag cagggauccg aaaggccauu aacagacacg      900

gaaagagcaa cauuaaugga ugaaccauau cguaaaagca aauuaacaua ugcacaagca      960

agaaaacuau uaggauuaga agacacagca uuuuucaaag guuuaaggua cgguaaagau     1020

aacgcagaag caccaacauu aauggaaaug aaagccuacc acgcgaucuc aagggcauua     1080

gaaaaagaag gccucaaaga uaagaaaucu ccauuaaauc ugucaacaga acuacaagau     1140

gaaauaggua cagcauucuc cuuguuuaaa acagauaaag acauaacagg acgacuaaaa     1200

gacagaguac aaccagaaau acuagaagcc cuacuaaaac auauaucauu cgacaaauuu     1260

guacagauuu cuuugaaagc acucagacgu aucguaccuc uaauggaaca aggaaaaaga     1320

uacgaugaag ccugugccga aauauaugga gaucacuauu gcaagaagaa ugcagaggag     1380

aaaauauauu uaccuccaau accugcagau gaaauuagga acccagucgu acuaagagcg     1440

cugagucagg cuaggaaagu aauaaauugc guaguacgaa gauauggauc cccagcuaga     1500

auccacauag aaacggcccg ugaaguaggu aaaaguuuua aagaccgaaa agaaauagag     1560

aaaagacagg aagaaaaccg caaagaccgg gaaaaagcag ccgcgaaauu ucgagaauau     1620

uuuccgaacu uuguaggaga accaaaaagu aaagauauau uaaaacuucg uuuguacgaa     1680

caacaacaug gcaaaugucu auauucaggu aaagaaauca accuuguccg auuaaaugaa     1740

aaagguuacg uagaaauaga ccacgcacua ccauucagua gaaccuggga cgacaguuuc     1800

aacaauaaag uacucgucuu aggaucagaa aaucaaaaua aagguaauca aacaccuuac     1860

gaauacuuca acgggaaaga uaauucacga gaauggcaag aguucaaagc aagaguugaa     1920

acuucacgau uuccgcgauc aaagaaacag aggauauuau uacaaaaauu cgaugaagaa     1980

ggauucaaag aacgcaacuu aaacgauacc agauauguua auagauuucu uugucaauuc     2040

guggcagacc auauucuauu aacuggcaag gguaaacgaa ggguuuucgc aaguaauggc     2100

caaauaacga aucuacuuag aggcuuuugg gguuuaagaa aaguaagggc ugaaaacgac     2160

agacaucaug cccuagaugc cguuguagua gcuuguagua cgguggcaau gcaacagaaa     2220

auaacuagau uuguucguua uaaggaaaug aaugcuuucg auggaaaaac aauagacaaa     2280

gaaacaggag aaguguuaca ucaaaaagca cacuuuccac aaccguggga guucuuugca     2340

caggaaguaa ugauccgggu auuuggaaag cccgauggaa agccagaauu ugaagaagca     2400

gauacaccag aaaaauuaag aacauuauug gcagaaaaac uaucaaguag acccgaagcg     2460

guccacgagu acguaacacc auuauucgug ucaagagccc caaauagaaa aaugagcgga     2520

caagggcaua uggaaacagu aaaaagugcg aagagguuag acgaagguau aagcguucuu     2580

agaguccccu uaacacaacu aaaacuaaag ggacuugaaa agaugguaaa ccgagaaaga     2640

gaaccaaaac uauaugacgc acuaaaagcc caauuagaga cacacaaaga uaaccccgcu     2700

aaggcuuuug cagaaccuuu uuauaaauac gauaaagcug gcaauaggac acaacaggua     2760

aaagcgguua gaauagaaca aguccagaaa accgguguau ggguucgcaa ucauaauggc     2820

auugcagaca acgcuaccau gguacgggua gacguuuucg aaaaaggggg caaauauuac     2880

cuaguaccaa uauauagcug gcaaguagcu aaaggaauau uaccagaucg agcaguuguc     2940

gcauuuaaag acgaagaaga cuggaccgua auggacgauu cguucgaguu cagauuuguu     3000

cuauaugcaa augacuuaau caaauuaaca gcaaagaaaa acgaauuucu cgguuacuuc     3060

guaucauuaa acagagcaac cggagcuaua gauauacgaa cacaugauac agacaguaca     3120

aaagguaaaa auggaaucuu ccaauccgua ggcguaaaaa cagcacucuc auuccagaaa     3180

aaucaaauug augaauuggg aaaagaaauu agaccaugua gauuaaagaa acgaccccca     3240

guaagauga                                                             3249


<210>  5030
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5030
auggcagcuu ucaagccuaa cccaauuaac uauauauuag ggcuugauau aggaaucgca       60

aguguagguu gggcaauggu agaaauagau gaagaagaaa auccaaucag auuaaucgau      120

cuagguguaa gaguauucga acgugcggag gugccuaaaa cuggagauag ucuugcuaug      180

gcacgacguc uagcaagauc aguacgaagg cucacacgaa ggagagcuca uagacucuua      240

agagcgagaa gacuauuaaa acgagaaggc guucugcaag cagcagacuu ugaugaaaau      300

ggccuuauaa aaagccuacc caauaccccg uggcaacuga gagcagcggc ccuagacaga      360

aaacuuacgc ccuuagaaug gucagccgug cuacuacauc uaauuaaaca uagagguuac      420

uuaagucaac gcaaaaacga aggagaaaca gcagacaaag aacuaggagc auuacuaaaa      480

ggaguggcaa acaaugcaca ugccuuacaa acuggugacu uuagaacucc ugcugaauua      540

gcauuaaaua aauuugaaaa agaaucugga cacauaagaa accaaagagg agacuauagu      600

cauacauuuu caagaaagga cuugcaagcg gaauuaauau uauuauuuga aaagcaaaaa      660

gaguucggaa acccacaugu gaguggcggc cucaaagaag gaauagaaac auuauuaaug      720

acacaaagac cugcucuuuc aggcgacgcg guccaaaaga ugcuuggaca cuguacauuu      780

gaaccagcag agccaaaagc ugccaaaaac acuuacacug cugaaagauu cauaugguug      840

acgaaacuca auaauuuacg aauacuagaa caaggaagug aaaggcccuu aacagauaca      900

gagagagcua cguuaaugga ugaaccguau agaaaaucaa aacuaacgua cgcacaagca      960

agaaaauuau uaggauuaga ggauaccgcc uuuuucaaag gacuaagaua cggaaaagau     1020

aaugccgaag caucgacacu cauggaaaug aaagcauauc augcaauauc acgugcauua     1080

gaaaaagaag gauuaaaaga uaagaaaagc ccucucaacc ucagcaguga acuacaagac     1140

gaaauaggaa cagcauuuag cuuauucaaa acagaugaag auauaacggg aagacugaaa     1200

gaucgcguac aacccgaaau uuuagaagcc uuauuaaaac acauuagcuu cgauaaauuc     1260

guccaaaucu cacuaaaagc acucagaaga aucgugccac uaauggaaca agguaagaga     1320

uaugaugagg cuugugcaga aauauacggu gaucauuacg guaagaaaaa cacugaagag     1380

aaaauauacu ugccaccuau accugcagau gaaauaagaa auccuguugu ucuuagggca     1440

uuaucacaag cacgaaaagu uauaaauggu guagugcgca gauauggauc cccagcuaga     1500

auccauauug aaacagcaag agaaguaggc aagagcuuua aagauagaaa agaaauugaa     1560

aaacgacaag aagaaaauag aaaagacagg gaaaaagcag ccgccaaauu cagagaguac     1620

uuuccaaauu uuguagggga accaaaaucg aaagacaucu uaaaguuacg acuuuacgaa     1680

caacaacaug gaaaauguuu auacucggga aaagaaauaa auuuaguaag auuaaacgaa     1740

aaaggauacg uagaaauaga ucacgcacua ccauuuucaa gaaccuggga cgacaguuuu     1800

aacaauaaag uauuaguauu aggcagugaa aaccaaaaca aaggaaacca aacccccuac     1860

gaauacuuua auggaaaaga uaauaguaga gaauggcaag aauuuaaagc aaggguagaa     1920

accagucgau uucccagauc uaagaaacaa agaauccuac uacaaaaauu ugaugaagac     1980

ggauuuaaag aaugcaaucu aaaugacacc cguuauguua acagauucuu augucaauuc     2040

guagcagauc acauauuacu cacaggaaaa ggaaaaagac gaguauucgc aucaaauggc     2100

caaauaacca aucuacuucg uggcuuuugg ggacuuagaa agguuagagc ugagaacgac     2160

agacaucaug cgcuugacgc cguaguagua gccugcucga caguugcaau gcaacagaaa     2220

aucacuagau uuguaagaua uaaagagaug aaugcauuug auggcaagac aauugacaaa     2280

gagacaggaa aaguauugca ucaaaagaca cauuucccuc aaccguggga guucuucgca     2340

caagaaguaa ugauacgagu auucggaaaa ccagacggaa aaccagaauu ugaagaggcu     2400

gacacaccag aaaaacucag aacauuacuu gcagaaaaac ugagcucaag accagaagca     2460

guacacgaau acgucacgcc ccuauuugua agcagggcuc caaaccguaa aaugucaggc     2520

caagggcaua uggaaacugu aaaaucagca aaacgauuag augaaggugu cuccgucuua     2580

cgaguuccau uaacacaauu aaaacuaaaa gauuuagaga aaauggucaa uagagaacga     2640

gaaccaaaac uauaugaagc acuaaaagcc cguuuagaag cucauaaaga cgauccagca     2700

aaagcauucg cugaaccauu cuacaaauau gauaaggcag gaaaccgcac acaacaagua     2760

aaagcuguaa gaguagaaca aguacagaaa acaggaguuu ggguacgaaa ucacaacgga     2820

auugcagaua augcaacaau ggugagggua gauguauucg aaaaaggcga uaaauacuau     2880

uuggucccua uuuacaguug gcaaguagcu aaaggaauau uaccagaccg agcaguagua     2940

caaggaaaag augaagaaga cuggcaacua auugaugaua gcuuuaauuu uaaauuuucu     3000

cugcauccaa acgaucuagu agaaguuaua acgaagaaag caagaauguu uggauauuuc     3060

gcaaguuguc aucggggcac cggaaacaua aauauacgua uucaugauuu agaucauaaa     3120

auaggaaaga acggcauucu agaaggaauc ggagucaaaa ccgcacuaag uuuccaaaaa     3180

uaucaaauag augaguuggg gaaagaaaua agaccaugca gacuuaaaaa gaggccuccu     3240

guaagauga                                                             3249


<210>  5031
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5031
auggcagcau ucaaacccaa cccgaucaau uacauauuag gcuuagauau cggaauagcg       60

aguguaggcu gggcuauggu ugaaauugac gaagaugaaa acccaauaug uuugaucgac      120

cuagguguac gcguguucga acgagcagaa gugccuaaga cuggggauag ucuugcuaug      180

gcaagacguu uagcaagguc aguaagacga uuaacacgac ggagagcaca uagauuacua      240

agagcaagac gauuacugaa gcgcgaagga gugcuacaag cugcugauuu ugaugaaaau      300

ggauuaauua agagcuuacc uaacacaccg uggcaauuac gagcagcugc ucuugauaga      360

aaacucacuc cuuuagaaug gagcgcugua cuauuacacu uaauuaaaca uagagguuac      420

cuuagccaaa ggaaaaauga aggagaaacu gcugauaaag aacuuggagc acuucucaaa      480

ggagucgcug auaaugcgca ugcacuccaa acuggagauu uucgaacccc ugcagaacuc      540

gcuuuaaaca aguuugaaaa agaaucagga cauauaagaa aucaaagagg agauuauagu      600

cacacauucu cucgaaaaga uuuacaagca gaauuaauac uauuauuuga gaaacagaaa      660

gaauuuggaa auccgcaugu cagcggcgga uuaaaagaag ggauagaaac ucucuuaaug      720

acacagagac cugcacuaag uggagaugcu guacagaaaa ugcuaggaca uuguacauuu      780

gaaccagcag aaccgaaagc agcuaaaaac acauauacag cagaaagauu uauuuggcuc      840

acuaaauuaa acaaucuuag gauacucgaa caaggaucag aaagaccacu aacagacacu      900

gaacgugcua cauuaaugga ugagccauac agaaaaucaa aauugacaua ugcacaagca      960

cgaaaacuac uuggucugga agacacagca uucuucaaag gacuuagaua uggcaaggau     1020

aacgcagaag caucuacccu aauggaaaug aaggcuuacc acgcaaucuc uagagcauua     1080

gagaaagaag gacuaaaaga uaagaaaagu ccauugaauc uaaguccgga acuucaagau     1140

gaaauaggua cagccuucag uuuauucaaa accgaugaag auauaaccgg aaggcuaaaa     1200

gacagaauac agccggaaau acuggaggcu uuacuaaaac acaucagcuu ugauaaauuc     1260

guacaaauua gcuuaaaagc cuuaagaaga auaguuccac uuauggaaca aggcaaaaga     1320

uaugacgagg cuugugcaga aaucuaugga gaccacuaug gaaagaaaaa uaccgaagag     1380

aaaauauauu uaccaccaau accagcugac gaaauccgaa acccaguagu acuaagagca     1440

cuuagucaag cucguaaagu aauuaaugga gucguucgac gauauggcag ucccgcacga     1500

auacacauug aaacagcccg cgaagucggc aaaucguuua aagaucgaaa agaaauugag     1560

aaaagacaag aagaaaauag aaaagacaga gaaaaagcgg cagcgaaauu ucgagaauau     1620

uuuccuaauu uugugggaga acccaaaucu aaagauaucu uaaaauuaag auuauacgaa     1680

caacagcacg ggaaauguuu auauaguggg aaagaaauaa acuuagguag acuaaaugaa     1740

aaaggauaug uagaaauuga ucaugcucua ccauucagcc gaacauggga ugauucauuc     1800

aacaauaaag uucuuguauu aggaagcgaa aaccagaaua aaggcaauca aacaccauau     1860

gaauacuuua acgggaaaga uaauucaaga gaauggcagg aguucaaagc acgaguagaa     1920

acaagcagau uuccgagaag uaagaaacaa agaauacuau ugcaaaaauu ugacgaagau     1980

ggauucaaag aaagaaaucu aaaugauacg agauauguca accgauuuuu augucaauuc     2040

guugcagauc guaugaggcu uaccggaaaa gggaagaaaa gaguuuuugc gucaaaugga     2100

caaauaacca aucuguuaag aggguucugg ggauugagga aagucagagc cgaaaaugac     2160

agacaucacg cacuagacgc aguuguugua gcuugcucga caguagccau gcaacaaaag     2220

auaacaagau uuguaagaua uaaagaaaug aacgcauuug auggcaaaac aauugauaaa     2280

gaaacaggug aaguacuaca ucagaaaaca cacuuuccuc aaccauggga auuuuucgca     2340

caggaaguaa ugauaagagu auuuggaaaa ccggauggua aaccagaauu ugaagaagca     2400

gacacaccag aaaaauuaag aacauuacug gcugagaaau uaucaucacg accagaagcu     2460

guucacgaau acguaacacc acuguuugua agcagagcac caaacagaaa aaugaguggu     2520

caaggacaua uggaaacugu uaaaucugca aaaagguuag augaaggagu uuccguccua     2580

cgaguuccac ugacucaacu aaaacuaaaa gaccuagaga aaauggucaa ccgcgaaaga     2640

gaacccaaau uauaugaagc acuaaaagca agguuagaag cacacaaaga ugauccagcc     2700

aaggcuuuug cagaaccauu uuauaaauac gauaaagcag gaaauagaac acaacaagua     2760

aaagcaguaa gaguggaaca gguacagaaa acaggaguau ggguacgcaa ccauaacgga     2820

auagcagaua acgcaacaau gguaagagua gauguauucg aaaaagguga caaauacuau     2880

uuaguuccaa ucuacaguug gcaaguugcc aaaggaauuu uaccugauag agcuguagua     2940

caaggaaaag acgaagaaga uuggcaacug auugaugaua gcuuuaacuu caaauuuuca     3000

uuacauccga augaccuagu agaaguuauc acaaagaaag caagaauguu cgguuauuuu     3060

gcaucauguc acagagguac agggaauaua aacauacgaa uacaugaccu ggaucauaaa     3120

auuggcaaaa augggauucu agaagggaua ggagucaaaa cagcauuaag uuuucaaaaa     3180

uaucaaauag acgaacuagg agaagaaauu agacccugca gacugaaaaa gagaccaccc     3240

guaagguga                                                             3249


<210>  5032
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5032
auggcagcau uuaaaccaaa cccuauaaau uauauauuag gucuagacau aggcauugcu       60

ucuguagggu gggcaauggu agaaauugau gaagaugaaa acccaauaug cuuaauugau      120

uuaggaguaa ggguauuuga aagagcugaa gucccaaaaa cuggcgauuc ccuagcaaug      180

gccagaagac uggcccgauc aguuagaaga uuaacuagac ggagagcaca cagauuguua      240

agagcaagaa ggcuccuuaa aagagaagga guacuacaag cggcagauuu ugacgaaaac      300

ggacuaauca aaucauuacc caacacacca uggcaauuac gagcugcagc ccucgaucgu      360

aaauuaacac cauuagaaug gagcgcuguc uuacugcauc uuauaaaaca uagaggauac      420

cucagucaaa ggaaaaauga aggagaaacg gcagacaaag aacugggagc uuuacucaaa      480

ggaguagcug acaaugcuca ugcauuacaa acaggagacu uccgaacccc ugcagaacuu      540

gcauuaaaua aauucgaaaa agaaagugga cacauaagaa accaaagagg ggacuauagc      600

cacacuuuuu cacgaaaaga uuuacaagcc gaacuaauau uacuauuuga aaaacaaaaa      660

gaguucggaa acccacaugu uucagguggc uuaaaagaag gaauagaaac acucuuaaug      720

acacaaagac cggcacuauc aggugaugca guccagaaaa uguuagggca uuguacauuu      780

gaaccagcug aaccuaaagc agccaaaaau acuuacacgg cagaaagauu cauauggcuu      840

acaaaauuaa acaaucuuag aauacuagaa caagggagcg aaagaccauu aacugauaca      900

gaacgagcua cgcucaugga ugaaccauac agaaagucaa aauugacgua cgcacaagcg      960

aggaaacugu uagggcuaga agauacugca uuuuucaagg gauuacgaua uggcaaagac     1020

aaugcugaag caucaacccu aauggaaaug aaagcauauc augcuaucuc ucgagcauua     1080

gaaaaggaag gacuuaaaga uaagaaaucu ccccuuaauu ugucacccga acuucaggau     1140

gagauaggua cugcguuuag ccuauuuaaa acugaugaag auauaacagg acgauuaaaa     1200

gacaggauuc aaccagaaau cuuggaagca uuacuuaaac auauaaguuu cgacaaauuc     1260

gugcaaauau cacuuaaagc acuaagaaga auagugccau uaauggaaca agggaaaaga     1320

uacgacgagg caugugcaga aauauaugga gaccacuacg gaaagaaaaa cacagaagag     1380

aaaauuuacu uaccgccaau ucccgcagac gaaauaagaa acccaguugu auuaagagca     1440

cugucacaag caagaaaagu caucaaugga guaguccgua gauacggaag uccugcaaga     1500

auacauauug aaacagcaag agaagucgga aaaucuuuca aagauagaaa agaaaucgaa     1560

aaacgacaag aagaaaauag aaaagacaga gaaaaggcag cagcaaaauu ucgagaauau     1620

uucccaaauu uuguaggaga accgaaaagc aaagacaucu uaaaauuaag acuguaugaa     1680

cagcaacaug gaaaaugucu auacucuggu aaagaaauca auuuaggucg auuaaaugaa     1740

aaaggauacg ucgaaauaga ucaugcacua ccauuuagca gaacauggga cgacucauuc     1800

aauaauaaag uauuagucuu agguagcgaa aaccaaaaua aaggaaacca aacuccguau     1860

gaauacuuca auggaaaaga uaauagcaga gaauggcaag aauuuaaagc acgaguugaa     1920

acaagcagau ucccaaggag caagaaacaa agaaucuuau uacaaaaauu ugacgaagau     1980

gguuuuaagg aacgaaauuu aaacgacaca agauacguga acagauuccu augccaauuu     2040

guagcagauc gcaugagguu aacugggaaa ggaaagaaac gaguauuugc aucuaacgga     2100

caaaucacua auuuauuaag aggauuuugg ggucuaagga aaguaagagc agagaacgau     2160

agacaccacg cgcuugaugc aguaguagua gcaugcagca cagucgcaau gcaacagaaa     2220

auuaccagau uuguaagaua uaaagaaaug aaugcguucg auggaaaaac uauagauaaa     2280

gaaacaggag agguacucca ucagaaaaca cauuucccac aaccauggga guucuucgca     2340

caagaaguaa ugauaagagu auucggaaaa ccagauggua aaccagaauu ugaagaagcc     2400

gacaccccag aaaaacuuag aacauuacuu gccgaaaaau uaaguaguag accugaagcc     2460

guacaugaau auguaacgcc uuuauuuguc uccagagcgc caaauaggaa aaugucagga     2520

caaggacaua uggaaacagu caaaucugca aaaagacuug augaaggagu aucaguauug     2580

cgagugccgu uaacacaacu aaaauuaaaa gaucuagaga aaaugguuaa ucgugaacgc     2640

gaaccaaaac uauaugaagc ccuaaaagca agacuugaag cacacaaaga ugauccugca     2700

aaagcauucg cagaaccuuu cuacaaauau gacaaagcag gaaaucgaac ucaacaagua     2760

aaggcuguca gaguggaaca agugcagaaa acaggaguuu ggguaaggaa ucauaacgga     2820

auagcagaua acgccacaau gguaagagua gacguauucg aaaaaggaga caaauacuac     2880

cuaguaccua uauacucaug gcaaguagcc aagggaauac uaccagaucg cgcaguuguc     2940

caaggaaaag acgaagaaga uuggcaacuu auugacgaca guuuuaauuu uaaauuuagu     3000

uugcauccaa augaucuugu ugaaguaauc acaaagaaag ccagaauguu ugguuacuuc     3060

gccagcuguc acagaggcac agguaauaua aauauaagga uucacgaucu ugaucacaaa     3120

auugggaaaa auggaauauu agaaggaaua ggaguaaaaa ccgcccuaag uuuccaaaag     3180

uaccaaaucg acgaauuagg aaaagaaaua agaccuugua gauuaaagaa aagaccgccc     3240

guaagauga                                                             3249


<210>  5033
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5033
auggcagcau uuaaaccaaa uccaauuaau uauauauuag gauuggauau aggaauagca       60

agugucgggu gggcuauggu ggaaauugau gaagacgaaa auccuauaug uuugauagau      120

cuuggaguac gaguauuuga aagagcugaa gucccuaaaa cgggugacuc acuagcagca      180

gcgaggcguc ucgcaagauc aguaagaaga uuaacacgaa gacgagcgca cagacuuuua      240

cgagcccgac gccuucuaaa aagagaaggu guacuucaag cagcggacuu ugaugaaaau      300

ggcuuaauaa aaucguugcc uaacacucca uggcaacuca gggcagcagc ucuagacaga      360

aaacucacac cacuagaaug guccgcugua uuacuacauc uaauaaaaca ccgaggauau      420

uuaagucaaa ggaaaaauga gggugaaacc gcagacaaag aacuaggagc auuacuuaaa      480

gguguagcag auaaugcuca cgcacuacaa acaggugacu ucagaacucc agcagaacua      540

gcacuaaaua aauuugaaaa agagaguggu cauauaagaa aucaaagagg ugauuauuca      600

cacacauuua guaggaaaga ccuacaagca gaauuaauac uacuauuuga aaaacaaaaa      660

gaguucggga auccacaugu aagcggcggu uuaaaagaag gaauagaaac ucuucuuaug      720

acacaacgac cagcucucag uggggacgca guccagaaaa ugcuugguca uugcacuuuu      780

gaaccagcug aacccaaagc ggcuaaaaau acuuacacag cagaaagauu cauaugguua      840

acaaaacuaa auaaucuacg aauccucgaa caaggcagcg aacggccuuu aacagauaca      900

gaacgagcga ccuuaaugga ugaaccuuau agaaaaagca aauuaacaua cgcacaagca      960

agaaaauuac ucggcuuaga agacacagcg uucuuuaaag gguuaagaua cggaaaagau     1020

aaugcagaag cgucaacacu uauggaaaug aaagcauacc acgcaauauc aagagcguua     1080

gaaaaagaag gacuuaaaga uaagaaauca ccacuaaauu ugucaccaga auuacaagau     1140

gaaauaggaa cugccuucag uuuauuuaaa accgaugaag auauaacggg aagauuaaaa     1200

gauagggugc aacccgaaau auuagaagcc uuauugaagc auauaucauu ugacaaauuu     1260

guacaaauau cauuaaaagc cuuaagaaga auuguaccgu uaauggaaca aggaaaaaga     1320

uaugaugagg cuugcgcuga aauauacggu gaucacuacg gcaaaaagaa uacggaagaa     1380

aagauauauu uaccaccaau accggcagac gaaauuagaa acccaguugu ucucagagca     1440

cuaucacaag caagaaaagu aauaaaugga guaguaagaa gauacggaag cccagcaaga     1500

auccauauag aaaccgcuag ggaaguagga aagucauuua aagauagaaa ggaaauagag     1560

aaaagacaag aagagaacag gaaagauaga gagaaagcag cggccaaauu cagagaauac     1620

uuuccaaauu ucguuggaga gccaaaaucg aaggacauac ucaaauuacg uuuauacgaa     1680

caacaacaug gaaaauguuu auacaguggc aaagaaauca aucuaggaag gcuaaaugaa     1740

aaaggauaug uugaaauuga ccaugcauug ccauuuucua gaacuuggga ugacaguuuu     1800

aauaacaaag uauuaguauu agggagcgaa aaucaaaaua aagggaauca aacaccauac     1860

gaauacuuua auggaaaaga uaauucaaga gaauggcaag aguucaaagc cagaguagaa     1920

acaagucgcu uuccuagaag caagaaacaa agaauauugc uucaaaaauu ugacgaagau     1980

ggcuucaaag aaagaaaucu aaacgauaca agauacguua auagauuucu uugucaauuc     2040

guagcagaua gaaugagguu aacagguaaa gguaagaaac gaguguuugc gucaaacggc     2100

caaauaacaa aucuacuuag aggcuuuugg ggauuaagaa aaguuagagc agaaaaugac     2160

cgacaccaug cucuggaugc aguaguaguc gcauguucca cuguagcaau gcaacaaaag     2220

auuacgaggu uugucagaua caaagaaaug aaugcauuug auggaaaaac cauagauaaa     2280

gaaacuggag aaguuuuaca ucagaaaaca cauuucccac aacccuggga guucuuugcg     2340

caagaaguaa ugauacgagu auuuggaaaa cccgacggaa aaccugaguu cgaagaagca     2400

gacacaccug aaaaacuacg aacccuacua gcggaaaaau ugaguaguag acccgaagca     2460

guacaugaau augucacacc acucuuugua aguagagcgc ccaauagaaa aaugucaggc     2520

caaggucaca uggaaacagu gaaaucagca aaacgcuuag acgaaggagu cucuguacua     2580

cgaguaccuc uuacacaauu aaaauuaaaa gaccuagaga aaaugguaaa uagagaacga     2640

gaaccgaaau uauacgaagc auuaaaagca agacuagaag cucauaaaga ugacccggcg     2700

aaagcauucg cagaaccguu uuauaaauac gacaaagcag gaaaucgcac ucaacaagua     2760

aaagcuguga gagucgaaca gguacagaaa acaggaguuu ggguaaggaa ucauaauggg     2820

auagcugaca augcaacaau gguaagggua gacguguuug aaaaagguga uaaauauuau     2880

uuaguaccaa uauauucaug gcaagucgca aaaggaauuu uaccagauag agcuguagua     2940

caagguaaag augaagaaga cuggcaacuc auagacgauu cguuuaauuu uaaauuuagu     3000

cuacacccaa augauuuggu agaagucauu accaagaaag caagaauguu cggguauuuu     3060

gcguccuguc auagaggaac aggaaauaua aauauacgua uacaugaccu ggaccacaaa     3120

auagguaaaa auggaauauu agaagguaua ggagucaaaa cugcauugag cuuucaaaaa     3180

uaccaaaucg augaacuagg aaaagaaaua agaccaugcc gcuuaaagaa aaggcccccg     3240

guaagauga                                                             3249


<210>  5034
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5034
auggcugcau uuaaacccaa uccaauaaau uacauacuug gccuagacau aggaauugca       60

ucuguaggau gggccauggu cgaaauugac gaggaagaaa auccaauuag acuuauagac      120

uuaggaguua gaguguucga aagagcagaa guaccaaaaa caggugauuc acuagcgaug      180

gcaagaagac uagcucgaag uguuagaaga cuaacgcguc guagagcaca ccgauuauug      240

agaacaagaa gguuacuuaa aagagaaggc guacuacaag cagcgaacuu ugacgaaaac      300

ggacuaauua aaagucuucc aaacacacua uggcaacuac gagcagcagc uuuagauaga      360

aaacuuacac cacuagaaug gucagcagua uuacuacauc uuauuaaaca cagagguuac      420

cuaagucaaa gaaagaauga aggcgaaacg gcagauaaag aacuaggagc acuucuaaaa      480

ggaguagcag gaaaugcaca ugcacuucaa acaggcgauu uuagaacacc cgcggaauua      540

gcauuaaaua aauuugaaaa agaaagugga cauaucagaa aucaaagagg agacuauagc      600

cauacguuua guagaaaaga ucuacaagca gaacucaacu uauuguucga aaaacaaaaa      660

gaguucggaa auccucacau aagcgacgga uuaaaagaag guauagaaac uuuauuaaug      720

acccaacgac cugcacuauc gggagaugcg guacagaaaa uguuaggaca uuguacauuu      780

gagccaacag agccuaaagc agcuaaaaac acauacacaa ccgaaagauu uaucuggcua      840

acaaaauuaa acaaucuccg aauacucgaa caagguucug aaagaccguu aacagauaca      900

gaaagagcua cucuuaugga ugagccuuau agaaaaucua aacugacuua ugcucaagcu      960

agaaaauugc ucgagcuaga ugauauugca uucuucaaag gauuaagaua uggaaaagau     1020

aaugccgaag cuaguacacu gauggaaaug aaagcauauc augcaauauc acgagcauug     1080

gaaaaagaag gauuaaaaga uaagaaaagc ccauuaaauc uaaguccaga auuacaagau     1140

gaaauaggga cggcauucag uuuauuuaaa accgaugaag acauaacagg ccgauugaaa     1200

gauagaauac aaccugagau auuagaggcu uuacucaaac auauuaacuu ugauaaauuu     1260

gugcaaauau cauuaaaagc acuaagacgu auaguaccuu uaauggaaca agggaaaaga     1320

uaugacgagg cuugcgcgga aauauaugga gaucauuaug gaaagaaaaa cacagaagag     1380

aaaauagauc uacccccaau uccagcagau gaaauacaua auccagucgu acuaagagca     1440

cuuucacaag cccgaaaagu aaucaaugga guaguucgca gguauggaag uccagcuaga     1500

auacacauag aaacugcgag agagguaggu aaaucuuuua aagaucgcaa agaaauagag     1560

aaaagacaag aagaaaacag aaaagacaga gaaaaagcag cagccaaauu ccgagaauac     1620

uuuccaaacu ucguaggaga accuaaauca aaagauauac ucaaauuaag acuuuacgaa     1680

caacaacaug gaaaaugucu auacagcgga aaagaaauca accuaggccg acuaaaugaa     1740

aaaggauacg uggagauuga ccacgcucuc ccauuuagca gaacauggga ugacucauuu     1800

aauaacaagg uuuuaguacu aggaagugaa aaucaaaaca agggaaacca aacaccauac     1860

gaguauuuua acggaaaaga caacucaaga gaauggcaag aauuuaaagc cagagucgaa     1920

acaagcagau uuccaagaag caagaaacaa cgaauauugu uacaaaaauu ugaugaagau     1980

gguuuuaaag aaagaaacuu gaaugauacc agauauguaa aucgauuuuu augccaauuu     2040

guagcugaca gaaugcgacu aacaggcaaa ggaaagaaaa gaguuuuugc aaguaaugga     2100

caaaucacua aucuacuuag gggauuuugg ggucuacgaa aaguuagagc ugaaaacgac     2160

cgccaucacg cauuagacgc aguagucgug gccugcagua caguggccau gcaacagaaa     2220

auaacuaggu uuguacgcua uaaagaaaug aacgccuuug acggaaaaac gauagacaaa     2280

gaaacgggag aaguacucca ucagaaaacc cauuuuccac aaccuuggga auuuuucgca     2340

caagaaguaa ugaucagagu auuugggaaa cccgauggga aacccgaauu ugaagaagca     2400

gacacaccag aaaagcuccg uacacuccuu accgaaaaau uaucaucaag accugaagcu     2460

guacaugaau auguaacacc ucucuucgua ucgagagcac ccaauagaaa aaugucagga     2520

caaggccaua uggaaaccgu uaagagugca aaaagauuag augaaggaau aucaguguua     2580

aggguacccc uaacucaacu aaaacugaaa gauuuagaga aaaugguuaa cagagaacga     2640

gaaccuaaac uauaugaagc ucuaaaagcc cgacuagaag cacauaaaga cgauccugca     2700

aaagcauuug ccgaaccauu uuauaaauac gauaaagcag gcaacagaac ccaacaagug     2760

aaggccaugc gaguggaaca aguacaaaag acaggaguau gggucagaaa ccacaauggc     2820

auugcagaua acgcaacaau ggugagagua gaugucuucg aaaaaggcga caaauacuau     2880

uuagucccca uauacaguug gcaaguagcc aaaggaauuu ugccugaucg agcaguagug     2940

cagggaaagg augaagaaga cuggcaacua aucgaugaua guuuuaacuu uaaauuuuca     3000

uuacauccaa acgaccuagu cgaaguaaua acuaagaaag cuaggauguu uggauauuuc     3060

gccucauguc acagaggaac cggaaauaua aacauuagaa uacacgaccu ugaucauaaa     3120

auugguaaaa acgggauacu agaaggaaua gguguaaaaa ccgcacuauc cuuccaaaag     3180

uaucaaauug augaacuagg aaaagagaua agacccugca gacuaaagaa aagaccacca     3240

guaagauga                                                             3249


<210>  5035
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5035
auggcagcau uuaaaccuaa cccuauuaau uacauucucg gccucgauau ugguauugca       60

ucagucggau gggcuauggu agaaaucgac gaagaugaaa auccaauaug uuuaauagau      120

cuaggaguaa gaguuuuuga aagagcagaa guaccaaaaa ccggagauag ccuagcgaug      180

gcaagaagac uagcaagauc aguaagacgu uuaacaagaa ggagggcuca uagguuacuc      240

agagcuagac gauuguuaaa aagagaaggc guauuacaag cagcagacuu ugaugaaaau      300

ggauuaauua aaagucuccc caauacgccc uggcaauuga gagcugcagc auuagaucga      360

aaacugaccc cacucgaaug gucagccgua cuauuacauc uaauuaaaca uagaggauau      420

cucagccaac ggaaaaauga aggggaaaca gcagauaaag aauuaggagc guuauugaaa      480

ggaguugcag auaaugcaca cgcacuacaa acaggagacu uuagaacacc agcagaauua      540

gcacuuaaua aauucgaaaa ggagucagga cauauaagaa accaaagagg cgacuauuca      600

cauaccuucu ccagaaaaga ucuucaagcu gaauugaacc uacuauucga aaagcaaaaa      660

gaguucggca auccucacau uucagauggc cuuaaagaag gaauagaaac acuauuaaug      720

acucaaagac cagcauuaag uggagacgcu guacagaaaa uguuaggaca uuguacauuu      780

gaaccggcag aaccaaaggc ugcuaaaaac accuauacgg cagaaagauu cauauggcuc      840

acaaaacuaa acaauuugcg aauuuuagaa caaggaucag agcgcccacu aacugacaca      900

gaacgaacua cauuaauaga ugaaccuuac cguaaaagua aguuaacaua ugcacaagca      960

cgaaaauugu uagaauuaga caauaccgca uuuuucaaag gacugaggua ugguaaagau     1020

aacgcagaag ccucaaccuu aauggaaaug aaagcauacc augcgauaag ccgagcauua     1080

gaaaaagaag gacuaaaaga uaagaaaagu ccauuaaauu uaucuccaga acuccaagau     1140

gagauaggga ccgcauucag ucuguucaaa acugacgaag auauaacagg cagacuaaaa     1200

gacagagucc aaccagaaau ccucgaagca uuauuaaaac auauaaguuu ugauaaauuc     1260

guccaaauau cauuaaaagc gcuaaggcgu auaguaccgc ucauggaaca aggaaaacgg     1320

uaugacgagg cuugugcaga aauauacgga gaucacuaug gaaagaaaaa uacugaagag     1380

aaaauugauu uaccacccau ucccgcggac gaaauaagaa acccagucgu uuuacgagcc     1440

uuaagucaag ccagaaaagu aauuaaugga guaguccgca gauacggauc gccggcuaga     1500

auacauauug aaacagccag agaaguggga aaaucuuuua aagacaggaa agaaaucgaa     1560

aaacgacaag aagaaaauag aaaagauaga gaaaaagcag ccacaaaauu cagagaauau     1620

uuuccaaacu uugucgguga acccaaaucu aaagacauac uuaaauuaag auuauaugaa     1680

caacaacaug gaaaaugcuu auacucagga aaagaaauua accuagguag gcugaacgaa     1740

aaagguuacg uagaaauaga ucaugcacua ccguucucac gcacauggga ugauaguuuc     1800

aacaauaaag ucuuaguccu cggaucagaa aaucaaaaua aaggaaauca aacaccauau     1860

gaauacuuca augguaaaga caauagcaga gaauggcaag aguucaaagc cagaguugaa     1920

acaucccgau uuccccgauc uaagaaacaa agaaucuuau uacaaaaauu cgacgaagaa     1980

ggauuuaaag aacgcaaccu aaacgacaca agauauguaa auagauuucu gugucaauuc     2040

guagcagauc auaugcuucu aaccggaaaa gguaaaagaa gaguuuuugc uucaaacggc     2100

caaauaacga auuuacuaag aggauuuugg gggcuaagaa aaguacgagc ugagaacgac     2160

agacaucacg cacuagaugc aguugucgua gcuuguagua cagucgcaau gcaacagaaa     2220

aucacacgau ucguacgaua uaaagaaaug aaugcuuucg augguaaaac uauagacaaa     2280

gaaaccggug aaguauuaca ucagaaaaca cacuucccac aaccauggga auuuuucgca     2340

caagaaguga ugauaagagu uuuugguaaa ccagacggaa aaccggaguu cgaagaagca     2400

gauacaccug aaaaacucag aacacuauua gcagaaaaac uaagcagcag accugaagcu     2460

guucacgaau acgucacacc acuauucgua agccgagcuc caaauagaaa aaugucaggu     2520

caagggcaua uggaaaccgu uaaaucagcc aagagauuag augaaggcgu caguguucua     2580

agaguucccc uaacacaauu aaaauuaaaa gacuuagaga aaaugguaaa ucgcgaacga     2640

gaaccaaaau uauaugaagc acugaaagca cgauuagaag cacauaaaga cgauccugcu     2700

aaagcauuug cagaaccuuu cuauaaauau gauaaagcag ggaauaggac ucaacaagug     2760

aaagccguaa gaguugaaca aguucagaaa acuggaguuu ggguaaggaa ucauaaugga     2820

auugcugaua acgcaacuau gguacgugua gacguauucg aaaaagguga uaaauacuac     2880

cuagucccga uauacucaug gcaagucgcc aaagguauuc uuccagacag ggcugucgua     2940

caaggaaaag acgaagaaga uuggcaacug aucgacgaua guuucaacuu uaaauucucg     3000

cuucauccga augaucuagu agaaguuaua acaaagaaag cucguauguu cggauauuuu     3060

gccucauguc accgcggaac aggaaauauu aacauucgua uucacgauuu agaccauaaa     3120

auuggcaaaa acgggauucu agaaggaauc ggaguuaaaa ccgcucucuc cuuucaaaaa     3180

uaccaaauag acgaauuagg caaagaaaua agaccaugua gauuaaagaa aaggccacca     3240

guaagauga                                                             3249


<210>  5036
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5036
auggcagcgu ucaaacccaa uccaaugaau uacaucuuag gacuagauau aggcauagcu       60

ucuguaggau gggcaauagu cgaaauagau gaagaagaaa aucccauaag acugauagac      120

uuaggaguac gaguauuuga aagggcugaa guuccaaaaa caggagauuc acuagcagca      180

gcaaggcgac uagcucgauc aguuagaaga cuaacaagaa gacgugcaca uagguuacua      240

agagcaagga gguuauuaaa aagagaaggc gucuuacaag cugcagauuu cgaugaaaac      300

ggacuaauaa aaucacuacc aaauacgccu uggcaauuac gugcugcggc auuagauaga      360

aaacuaacac cgcuugaaug gucagccgua uuacuccacc uuaucaaaca uagaggguac      420

cuaucucaaa gaaagaauga aggggagacc gcugauaaag aguuaggagc uuuacugaaa      480

gguguggcag auaacacaca cgccuuacaa acaggagacu ucagaacccc ugcagaacua      540

gcacucaaca aauuugaaaa agagucuggu cauauuagaa accaacgagg agacuauuca      600

cauaccuuua acagaaaaga cuuacaagcg gaacuuaacc uauuauuuga aaaacaaaaa      660

gaguucggaa acccacacgu gagcgacgga cuaaaagaag guauugaaac guuauuaaug      720

acacaacgac cagcacuauc aggagaugcu guucagaaaa ugcucggaca uugcacauuc      780

gaaccaacgg aacccaaagc agcuaaaaau acuuacacag cugaacgauu cguuugguua      840

acuaaacuaa acaauuuaag aauauuagaa caaggaagug aaagaccccu aacagauacu      900

gaaagggcua ccuuaaugga cgaaccauac cgaaaaagca aacuuaccua cgcacaagca      960

agaaaacuac uagaucuaga cgacacagca uucuuuaaag gacuuaggua cggaaaagau     1020

aaugcagaag caucaacacu cauggaaaug aaagcauauc augcuauaag cagagcauua     1080

gagaaagaag gguuaaaaga caagaaaucc ccgcuuaauc uuaguccuga auuacaagac     1140

gaaauaggaa cugcauuuuc cuuauuuaaa acagacgaag auauaacagg uagauuaaaa     1200

gauagaguac aaccagagau auuagaggcc cuauuaaaac auaucagcuu ugauaaauuu     1260

gugcaaaucu cauuaaaagc acuucgccga auuguaccau uaauggaaca ggguaacaga     1320

uacgacgaag caugcacuga aauauauggc gaccauuacg gaaagaaaaa cacagaagag     1380

aaaauuuacu uaccuccaau accagcugac gaaauccgua auccuguagu acuacgagca     1440

cuuagucaag cacgaaaagu aauaaacgga guaguuagaa gauacggauc uccagcucga     1500

auacacaucg aaacagcacg ugaaguaggu aaaucguuca aagaucggaa agaaauugag     1560

aaaagacagg aagaaaacag gaaagaccgc gagaaaagug cugcuaaauu cagagaauac     1620

uuuccuaauu uugucggcga accaaaaagc aaagacauuu uaaaacuccg acuauacgaa     1680

caacaacacg gaaaaugucu uuauagugga aaagaaauaa accucgguag acuaaaugaa     1740

aaaggauacg uagaaauaga ucaugcucuc ccuuuuagua gaacguggga cgauaguuuu     1800

aauaacaaag uacuagcauu aggaucugaa aaccaaaaua aagguaauca aacuccuuac     1860

gaauauuuua auggaaaaga uaauucaaga gaauggcaag aauuuaaagc aagagucgaa     1920

acuucacgcu uuccaagauc aaagaaacaa agaauacugc uccagaaauu ugaugaggac     1980

ggauuuaaag aacggaaucu aaaugacacu agguauauaa auagguuuuu augccaauuu     2040

guugcagauc acaugcuauu gacuggcaaa ggaaaacgaa gaguauuugc gagcaaugga     2100

caaauaacaa acuuauuaag aggcuuuugg ggauugagaa aaguacgagc ugaaaaugac     2160

cgucaccaug cguuggaugc aguuguagua gcauguucua cgauagcgau gcaacagaaa     2220

auaacccggu uuguaagaua caaagaaaug aaugccuucg augguaaaac cauagauaaa     2280

gaaacaggag agguacucca ucaaaaagca cauuuccccc aaccauggga guucuuugcg     2340

caagaaguaa ugauaagggu auucggaaaa ccagauggua aaccggaauu ugaagaagca     2400

gauacacccg aaaaauuacg aacucuauua gcagaaaaac uuucauccag acccgaagca     2460

guacauaaau acgugacacc guuauuuaua ucuagagcac caaaucgcaa aaugucuggg     2520

caaggacaca uggagaccgu aaaaucagca aagagauuag acgaaggaau cucuguacua     2580

agagucccau uaacucaauu aaaauuaaaa gacuuagaga aaauggucaa cagggaaaga     2640

gaaccaaaau uauacgaagc ucuaaaagcu cgacuagaag cacacaaaga ugaucccgcu     2700

aaagcauucg cggaaccauu cuacaaguac gauaaggccg gcaacaggac ucaacaagua     2760

aaagcuguaa gaguagaaca agugcagaaa acaggaguau ggguucauaa ucauaaugga     2820

auugcugaca augcaacgau aguaagagua gacguuuuug aaaaaggugg uaaauauuac     2880

cuaguuccua uuuauucaug gcaaguagcu aaagguaucc uuccggacag agcuguugug     2940

caaggaaaag augaagaaga uuggacagua auggaugaca gcuuugaauu uaaauuugua     3000

cuauacgcaa augauuuaau uaaauuaacg gcuaaaaaga acgaauuuuu agguuauuuc     3060

gucucauuaa auagagcaac gggagcaaua gauaucagaa cccaugauac agacucuacc     3120

aaaggcaaaa acggaauauu ucaaucagua ggaguuaaaa ccgcacuaag uuuucaaaaa     3180

uaucaaauag acgaacuagg aaaagaaaua agaccaugcc gacuuaagaa aagaccuccu     3240

gucagguga                                                             3249


<210>  5037
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5037
auggcagcau uuaaacccaa uucaauuaau uacauacucg guuuggauau cgguauugcc       60

aguguagguu gggcaauggu cgaaauagac aaagaagaaa acccaauccg acuuauagau      120

cuaggaguua gagucuucga aagggcagaa guaccuaaaa caggugauuc cuuagcagcc      180

gcacgccgac ucgcaagguc agugagacga cuaacaagaa ggagagcuca caggcuacuc      240

agaacaagaa gguuacugaa aagggaagga guucuacaag cagcgaacuu ugauaaaaau      300

ggauuaauaa aaucuuuacc aaacacauua uggcaauuac gagcggcugc acuagauaga      360

aaacuaacgc cacuugaaug gucagcagua cuacuacacu uaauaaaaca uaggggauau      420

uugagccaac gcaaaaacga aggagaaaca gcggacaaag aauugggagc auuacuaaaa      480

ggagucgcag gaaacgccca ugcucuacaa accggugauu uuagaacccc ugcagaacua      540

gcacuaaaua aauucgaaaa agaaucagga cauauacgua aucaaagagg agacuauuca      600

cacacauuuu caagaaaaga uuuacaagcu gaacuuaauu uacucuuuga gaaacagaaa      660

gaauuuggua acccccauau aaguggaggc cucaaagaag ggauagagac auuacuuaug      720

acccaaagac cggccuuaag uggggacgca guacagaaaa ugcuggguca cugcacauuu      780

gaaccaacug aaccaaaagc agcuaaaaau acuuacacaa ccgaacguuu cauuuggcua      840

accaaauuaa auaacuuaag gauuuuagag caaggaucag aaagaccauu aacagauaca      900

gaaagagcaa cacucaugga cgaaccauac cgaaaaucaa aauuaacaua cgcacaagca      960

agaaaacuac ucucucuaga agauacagcc uuuuucaaag gauuaaggua uggcaaagau     1020

aacgccgaag cgucaacuuu aauggaaaug aaggcguacc acacaauuag cagagcacua     1080

gaaaaagaag gguuaaaaga uaagaaaucu ccucuaaauc uaucucccga acuacaagac     1140

gaaauaggua cugcguuuag ccuauuuaaa acagacgaag auauaacagg aagguuaaaa     1200

gacaggauac aaccagaaau auuagaggca uuauuaaaac acauaaguuu cgauaaauuc     1260

gugcaaauau cauuaaaagc ucuaagaaga auaguuccuc uuauggaaca agguaaaaga     1320

uacgaugaag caugcgccga aauuuauggu gaccauuacg ggaagaaaaa uacagaagag     1380

aaaauauauu uacccccaau accagcggau gaaauaagaa auccagucgu acucagagcg     1440

uuaagucaag caagaaaggu uauaaauggu guggugagac gauauggaag uccugcaaga     1500

auacauaucg aaacagcaag ggaaguagga aaaucauuua aagacaggaa agaaauagaa     1560

aagagacaag aagaaaauag gaaagaucgg gaaaaagcug ccgcaaaauu ccgugaauac     1620

uucccaaauu ucguggggga accaaaauca aaggauauac uaaaauuaag acuuuaugaa     1680

caacaacaug gaaaaugccu auauucaggc aaagaaauua aucucguaag acuaaacgaa     1740

aaaggcuaug uugaaauaga ucacgccuua ccguuuucca ggacuuggga cgauaguuuu     1800

aacaauaaag uuuugguccu uggcucagaa aaccaaaaua aaggcaauca aacaccauau     1860

gaauacuuua auggaaaaga caacucgagg gaauggcaag aauuuaaggc aagaguagaa     1920

acaucacguu uuccuagauc caaaaagcaa agaauauuac uccaaaaauu cgacgaagac     1980

gguuucaaag aaagaaaccu uaacgacacg cgauauguua auagauuucu uugccaauuu     2040

guagcagacc acauuuuacu aacaggcaaa ggaaaaagaa gaguguuugc gucaaaugga     2100

caaauaacua acuugcuacg cgguuuuugg ggacuacgaa aaguacgcgc ugaaaacgau     2160

agacaccacg cccuggaugc aguuguagua gcauguucaa cagucgccau gcaacaaaag     2220

auaaccagau ucguucgaua uaaagaaaug aaugcauuug auggaaaaac gauagauaaa     2280

gaaaccggag aaguccuuca ucagaaaacu cacuuuccac aaccauggga guucuucgca     2340

caagaaguaa ugauucgugu uuuuggaaaa ccagauggaa aaccagaauu ugaagaagcc     2400

gauacuuuag aaaaacuaag aacauuguua gcagaaaaau ugucaagucg uccugaagca     2460

guacaugaau augucacacc gcucuuugua ucucgagcac caaauagaaa aauguccggc     2520

caagggcaca uggaaacagu aaaaucggca aaaagacuag acgaaggagu gucaguauua     2580

agagucccgu ugacacaauu aaaauugaaa gaucuagaga aaaugguaaa ucgcgaaaga     2640

gaaccaaaac uauaugaagc cuuaaaagca agacuagaag cacacaaaga ugacccugca     2700

aaagcauucg cugaaccauu cuacaaauau gacaaggcgg gaaacagaac acaacaaguc     2760

aaagcaguac gaguagaaca agugcagaaa accggaguau ggguccgaaa ucauaacgga     2820

auagcagaca augcaacgau gguuagaguu gacguuuuug aaaaaggaga uaaauauuau     2880

uugguaccaa uuuauucaug gcaaguagca aaaggaauau uaccagauag agcaguagua     2940

caaggaaaag acgaagaaga uuggcaacua auugacgacu cauuuaacuu caaauuuagu     3000

cuucauccga augacuuagu agaaguaaua acaaagaaag cuagaauguu cggauauuuu     3060

gcuucauguc acagagguac aggaaauaua aacauaagaa uacacgaucu ggaucacaaa     3120

auagggaaaa acggaauacu agaaggaaua ggagucaaaa cggcccucuc uuuccaaaaa     3180

uaucagauag augaauuagg aaaagaaaua agaccaugua gacuuaagaa aagaccccca     3240

guuagguga                                                             3249


<210>  5038
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5038
auggcagcau uuaaacccaa ucccauaaac uacauucuag gacuagacau uggaauagcc       60

ucaguaggau gggcaauggu cgagauagau gaagaagaaa accccauaag acuaaucgau      120

uuaggaguac gagucuuuga aagagcggaa guuccuaaaa caggagacuc acuagcuaug      180

guuagaagac ucgcaaggag uguaagaagg cugacuaggc ggagagcaca uagacuccuc      240

agagcuagac gauuauuaaa acgcgaagga gucuuacaag cagccgauuu ugaugaaaac      300

ggacuaauaa aaucuuuacc aaauacaccc uggcaacuac gggcggcggc acuagacaga      360

aaacucacac cauuagaaug gagugcaguu cuauugcauc uaauuaagca ucggggauac      420

uuaucacaac guaaaaacga aggagaaaca gcagauaaag aauuaggugc ucuauuaaaa      480

ggaguagcag acaacgcaca ugcacuucaa acaggcgauu ucagaacucc ggcagaacua      540

gcauugaaua aauucgaaaa agaaagcggc cacaucagga accaaagagg agauuauagc      600

cacacuuuca gccguaaaga ccuccaagca gaacucauau uacuuuucga aaaacaaaaa      660

gaguuuggua acccucauau auccggggga cuaaaagaag gaauugaaac ucuauuaaug      720

acacaaagac cagcucuauc cggagaugca gugcagaaaa uguuaggaca uugcacauuc      780

gaaccugcag aacccaaggc agcuaaaaau acauauaccg cagaaagguu uauuuggcua      840

acaaaauuaa auaauuuaag aaucuuagaa caaggaagug aaagaccucu gacugauaca      900

gaacgugcaa cauugaugga cgaaccauau cgcaaaucaa aacugacaua ugcacaagca      960

cgcaaauuau uaggauuaga agacacggca uuuuucaaag gacuuagaua cggaaaagau     1020

aaugccgaag caagcacauu aauggaaaug aaagcauauc augcaauaag ccgcgcauua     1080

gaaaaagagg gacuaaagga caagaaaagu cccuugaauc uuaguccgga auugcaagac     1140

gaaaucggaa cagccuuuuc acuauuuaaa acagaugaag acauaacugg aagauuaaaa     1200

gaucgaauac aaccugaaau acuggaagca cuauuaaaac auaucaguuu cgauaaguuc     1260

guacaaauaa guuuaaaagc acuaagaagg auugucccac ucauggaaca aggaaaaaga     1320

uaugacgaag caugugcaga aaucuacgga gaucauuaug gcaagaaaaa cacugaagag     1380

aaaauauacu uaccaccuau uccggcagau gaaauacgua acccuguggu auuacgagca     1440

cuaucacaag cuaggaaagu aauaaaugga guaguccgaa gauauggauc uccagcacgc     1500

auucacauag aaaccgcaag agaaguaggu aaaucauuua aagacagaaa agaaauagaa     1560

aaacgucaag aagaaaacag aaaagauaga gaaaaggcag cggcaaaauu uagagaauau     1620

uuucccaauu uuguaggaga gccuaagagu aaagacauau uaaaauuacg ccuauacgaa     1680

caacaacaug gcaaaugucu auauagcgga aaagaaauaa auuuaggacg ucuaaaugaa     1740

aaaggcuaug uagaaaucga ucacgcauua ccauuuucac gaacuuggga cgacucauuu     1800

aacaacaaag uauuaguauu aggaagcgaa aaccaaaaua aaggaaacca aacgcccuau     1860

gaauauuuua acggaaaaga uaacaguaga gaguggcaag aguucaaagc ccgaguagaa     1920

acaucacgau uuccgagaag caaaaagcag cgaauacuuu uacaaaaauu ugacgaagau     1980

ggauucaaag aaagaaauuu aaaugauaca agauauguua acagauuucu uugucaauuu     2040

guagcagauc gaaugcgucu aacaggaaaa gguaagaaaa gaguuuuugc uucaaauggc     2100

cagauaacua acuuacuuag aggauuuugg ggucuucgua aaguaagggc ugaaaaugac     2160

cggcaucaug cacuagacgc aguugucguu gcaugcucaa caguagcaau gcaacagaaa     2220

aucacucguu ucgugcgaua caaagaaaug aacgcauuug auggaaaaac aauagauaaa     2280

gaaacaggag aaguuuuaca ccagaaaaca cacuuuccac aaccuuggga guucuuugcc     2340

caagaaguaa ugauuagagu auuuggaaaa ccagacggaa aacccgaauu ugaagaagcg     2400

gauaccccag aaaaacucag aacacuuuua gcagaaaaac ucucaucaag accagaagca     2460

guacacgaau acguaacucc auuauuuguu agccgagcac caaauagaaa aaugucaggu     2520

caaggacaca uggaaacagu caaaucugcu aaacgacuug augaaggagu aucuguacua     2580

cgcguuccac ucacacaacu aaaacuuaaa gaccuagaga aaauggucaa ucgcgaaaga     2640

gaaccuaaac ucuaugaagc acuaaaagca agacuagaag cacauaaaga ugacccagca     2700

aaagcguuug cggaaccauu cuacaaauau gauaaagcag ggaacaggac acaacaagua     2760

aaggcuguaa ggguagaaca aguucagaaa acuggaguau ggguccgcaa ucauaacgga     2820

auugcagaca acgcuacaau gguaagaguu gauguauuug aaaaaggcga uaaauacuac     2880

uuaguaccaa uuuauucaug gcaaguagcc aaaggaauau uacccgacag agccgugguu     2940

caaggaaagg acgaggaaga cuggcaacuc auagaugacu cuuucaacuu caaauuuagu     3000

cuccauccaa acgauuuagu ugaaguaaua acuaagaaag caagaauguu cggguacuuc     3060

gcaucuuguc acagaggaac aggaaacauc aacauccgaa uacaugauuu agaccauaaa     3120

auagguaaaa acggaauauu agaaggaaua ggagucaaaa cggcacucuc guuucaaaaa     3180

uaucaaauag augaacucgg aaaagaaaua cggcccugca gacuaaagaa aaggccaccc     3240

guaagauga                                                             3249


<210>  5039
<211>  3249
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5039
augagaguuc uaggauuaga ugcgggaaua gcgucauuag gcugggcacu aauagaaaua       60

gaagaaagua auagagggga acuaagccag ggaacuauaa ucggcgcagg aacguggaug      120

uuugaugcgc cagaagagaa aacacaggcg ggugcaaaac uaaaaucaga acaacgaaga      180

acauuuagag gacaaaggag gguagucaga cgcagaagac aaagaaugaa ugaaguaaga      240

cgaauuuuac acagucaugg acucuugcca ucaucagaca gggacgcuuu gaaacaaccc      300

ggauuagacc cguggagaau aagagcagaa gcacuugauc gacuuuuagg accaguagaa      360

cuagcuguug cacuaggaca uauagcacga caucggggau ucaaaucaaa uaguaaagga      420

gcuaagacua augacccagc agacgauaca ucgaaaauga aaagggcugu gaaugaaaca      480

cgugaaaaau uagcgagauu uggaucagca gccaaaaugc uaguggagga cgaaaguuuu      540

guuuuaagac agacaccaac gaaaaauggg gcaagugaaa uagucagaag guuuagaaau      600

agagaagggg auuacaguag aucacuucua agagaugauu uggcggcaga gaugagagcc      660

cuuuuuacag cacaagcuag auuucaauca gcuauagcua cugcugauuu acaaaccgca      720

uucacaaaag cagcuuucuu ucaaaggcca cuacaagauu cagagaaauu aguuggucca      780

ugcccuuuug aaguugacga gaaaagagcu ccaaaaagag gauacucguu ugaacuuuuu      840

agguuuuuaa gccgacuuaa ccauguaaca cucagagacg ggaagcaaga aagaacuuug      900

acccgagaug aauuggcuuu agcagcagca gauuuuggag cagcagcaaa aguaucauuu      960

acagcacuaa gaaagaaacu aaaauuaccu gaaacaacug uuuuuguugg aguaaaagcc     1020

gacgaagaaa guaaauugga uguaguggcc agaucuggua aggcagcaga gggaacugca     1080

agauuacguu caguaauagu agaugccuua ggagaauugg cuuggggagc auuauuaugc     1140

ucaccagaaa aacuagauaa aauugcagag guaauaucau uccgaagcga cauaggacga     1200

auaucagaag guuuagcaca agcaggaugu aacgcgccuu uaguugacgc acuuacagca     1260

gcggcuucug auggucgguu ugacccguuc acaggcgcag gacauauuuc gagcaaggca     1320

gcaagaaaca uacugucugg gcuaaggcag gggaugacau augauaaagc guguugcgca     1380

gcagauuaug accacacagc uucaagagag agaggugcau ucgauguagg uggccacgga     1440

agagaggcau uaaaaagaau uuuacaggaa gagaggauaa guagagaauu aguaggcucc     1500

ccaacagcca gaaaggcgcu aauugaaagu auaaagcaag uaaaagcuau uguggaaaga     1560

uauggggugc cagauagaau acauguagaa uuagcucgag auguaggaaa aucaauugaa     1620

gaacgagaag aaauaacuag aggaauagaa aaacgaaacc gacaaaaaga uaaacuuaga     1680

ggauuauuug agaaagaagu uggaaggcca ccacaagaug gggcaagagg aaaggaagaa     1740

uuauugaggu uugaauuaug gagugaacaa augggaagau gucucuauac agaugauuac     1800

auaucaccau cacaauuggu ugcaacagac gaugcaguac aaguagacca uauauugcca     1860

uggucacgau uugcugauga uucauaugcg aauaaaacau uauguauggc caaagcaaac     1920

caggauaaga aaggaagaac cccauaugaa ugguucaaag cggagaaaac cgacacagaa     1980

ugggacgcgu ucaucguaag aguggaagca cuugcagaua ugaaaggauu uaagaaaaga     2040

aauuauaaac ucagaaacgc agaagaagca gcugcaaaau ucagaaacag aaauuuaaac     2100

gauacucgau gggcgugcag acucuuggca gaagcauuaa aacaauugua uccaaaagga     2160

gaaaaagaua aagacggaaa agaaagacga aggguguuuu caagaccagg agccuuaacu     2220

gauagauuac guagagccug gggauuacaa uggaugaaga aaucaacaaa aggagaccgc     2280

auuccggaug acagacauca ugcucuugac gcgaucguaa uagcugcaac aacagaauca     2340

cugcugcaga gagcaacaag ggaagugcaa gagauagaag auaaagggcu acauuacgau     2400

cuagucaaaa auguaacucc accuuggcca ggauucaggg aacaagcggu ugaggcugua     2460

gaaaaaguau uuguggcgag agcugaaagg agaagggcga ggggaaaagc ucacgaugca     2520

acgauaaggc auauagcagu aagagaggga gaacaacggg uuuacgagag aagaaaaguu     2580

gcugaauuaa aauuagcuga cuuagauagg guaaaagacg ccgaaagaaa cgcuagauua     2640

auagaaaaac uaaggaacug gauugaagcu ggaaguccaa aagaugaucc cccauuaagu     2700

ccaaaaggag auccaauauu caaaguaagg cuaguaacaa aaucaaaagu gaacauagca     2760

uuagacacag gaaacccaaa acgaccugga acagucgauc gaggcgaaau ggcacgagug     2820

gauguuuucc gcaaagcuag uaagaaagga aaauaugaau acuaucuagu accuauauau     2880

ccacacgaua uagcaacaau gaaaacaccu ccaauuagag ccguacaagc auauaaacca     2940

gaggaugagu ggccagagau ggauucauca uaugaguucu gcuggucuuu aguaccaaug     3000

acguaucuac aaguuauaag uagcaaagga gaaauauuug aaggguauua cagaggaaug     3060

aauaggucug uuggugcgau acagcuaagc gcccauucaa auaguucaga uguugugcag     3120

ggaauaggag ccagaacguu aacugaguuc aagaaauuca acguagacag auucgggaga     3180

aaacacgaag uagaacgaga auuaagaaca uggagaggag aaaccuggcg agggaaagcg     3240

uauauuuga                                                             3249


<210>  5040
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5040
augaaaaaca ccuuauaugg aauaggcuua gacauaggag uagcuucugu aggaugggcu       60

guaguaggac uaaauggcac uggcgaacca guaggucugc aucgucucgg aguacgaaua      120

uucgauaaag ccgaacagcc uaaaacuggu gaaucauuag cugccccgag aagaauggca      180

aggggcauga gacgaagacu caggagaaaa gcgcuaagga gagccgaugu cuaugcucua      240

cuagaaagau caggccuauc gacaagagaa gcauuagcac acauguuuga agcgggcggc      300

cuagaagaua uauaugcacu aagaacccga gcauuagaug aaccaguggg uaaagcagag      360

uucagccgaa uuuuacuaca cuuggcucaa agaaggggau uuaaaucaaa ccgaagaaca      420

gcuagugaug gcgaagaugg cagauuguug gccgcuguga acgaaaaucg cagaagaaug      480

gcacagggag gguggagaac cgucggagaa augcuauaua gacaugaagc auuugcacua      540

cauaaaagga auaaagcaga cgaauaccua ucaacgguag gaagagauau ggucgcugaa      600

gaagcaaguc uacuauuuca aagacaaagg gaguuggggu gugccugggc aacaccagaa      660

uuacaagcag aauaucuguc aauacuacuu agacaacgau cauuugacga aggaccaggg      720

ggaaacucac cauauggagg uaaucaaguc gagaaaaugg uaggaagaug uacguucgaa      780

cccgaugaac caagagcugc aaaagcggca uauagcuucg aauacuucag ccucuuacag      840

aaaaucaauc acaucagauu gguagaaaau ggugaaacaa ggccacuaac ucaacaacaa      900

cgucagcaau uacuaagucu cgcacacaaa acaccugaug ucagcuuagc cagaauaagg      960

aaggaacuug cacucccgga aacggugcaa uucaacggag ugagaugcag agcaaacgaa     1020

acucuagaag aaagugaaaa gaaagaaaaa uuugcguguc ugcccgcaua ucauaaaaug     1080

agaaaagccu uagacggagu aguuaaagga agaaucagcu cucuaaguau cagucaaaga     1140

gaugcagcag ccacagcucu uagucuauac aaaaaugaag auacauuacg agcaaaauua     1200

acagaagcag gcuuucaagc uccagaaauc gaugcucuag cuggauuaac cggguucucu     1260

aaauuuggac aucuaagucu gaaagcaugu agaaaauuaa uaccucaucu ugaacaagga     1320

cuaaccuacg aucaagccug uagugcagcc ggguaugacu uuaaagggca uggcgcuggu     1380

gaaagagcau ucacacuacc ugcagcagca ccagaaaugg aacaaauaac uucuccagua     1440

guucgaaggg caguagcaca aacaauaaaa gucguuaacg ggaucauaag agaaauggac     1500

gcaucaccgg cauggguuag aauagaacua gcgcgcgaau uauccaaaac auuuggggaa     1560

agacaagaaa uggaucguuc gaugagagaa aacgccgcac agaacgaaag acuaaugcaa     1620

gaacuucgug acacauuuca ucugcuauca cccacugggc aagaccuugu aaaauauaga     1680

cuuuggaaag aacaagaugg aguuugcgcu uacucacuaa gacguuugga cguagaaaga     1740

cuauuugaac cgggcuacgu agacguugau cacauaguac cauauucacu uagcuucgau     1800

gauagacgcu caaauaaagu ucucgugcuc ucaucagaaa aucgucaaaa agguaauaga     1860

uuaccauuac aguaucugca aggaaaaaga agagaggacu uuauuguaug gacgaacagu     1920

agcguuagag acuaccgaaa aagacaaaau cucuuaagag aaaaacuguc aggcgacgaa     1980

gcagaggggu uucggcaaag gaauuugcaa gauacucaac auauggcaag auuuuuauau     2040

aauuacauaa gugaucaucu ggcauucgcu caaucagaag cccuuggaaa gaaacggguc     2100

uuugcaguca gcggagcugu gacaagucau cuucgcaaac gguggggacu uagcaaagua     2160

agagcagaug gagauuuaca ucaugcacug gaugcggcag ucauagcuug uaccacagau     2220

ggcaugauac gaagaauaag cggauacuac ggacacauug agggagaaua ccuacaagau     2280

gcagacggag caggaagcca acacgcaaga acuaaagaac guuuuccugc accuuggccc     2340

cgauuucgag augaacuaau aguaagacua agcgagcaac caggagaaca ucugcuagau     2400

auaaauccgg aguucuauug ugaauauggg acagaacaca uaagaccagu auucguauca     2460

cgaaugccua gaagaaaagu cacuggccca ggacacaaag aaacgauaaa gggagccgcc     2520

gcugcagaug aaggccuacu uacaguaaga aaagcucuaa cagaacuaaa acuugauaag     2580

gauggagaaa uuaaagacua uuacaugcca agcagugaua cguuauuaua cgaagcacuc     2640

aaagcucaau uacgacgcuu uggaggagau agcaagaaag cauuugcaga accauucuac     2700

aaaccaaaag ccgaugguac cccaggaccu cuaguaagaa aaguaaaaac uacagaaaaa     2760

gccacacuaa ccguuccagu gcauggagga gcugcaucaa acgacacuau gguaagagua     2820

gauguauuuu uggugccagg agacggauac uauugggugc cgguauauac agcggacacg     2880

cuuaaaccag aacuaccaaa cagggcagua guacaaggaa aaccuuauuc ugaauggaaa     2940

gaaaugcgag aagaagacuu uauauuuagc cuauacccaa acgaucuagu auauguugaa     3000

cacaaaucag ggcuaaaauu caccuuacag aacgcagaua guacauuaga gaaaacccga     3060

acacaaaccu cggcauuugu auauuuugua ggugguucaa uaagcacagc uucaauaaca     3120

auaagaacac augacaaugc uuacggcauc ccaagccuag gaguuaaaac uuuaaaaacc     3180

uuccauaaau aucaaguaga uguauuagga aauguauucc caguacauaa agaaacaaga     3240

caaaaauuua gguga                                                      3255


<210>  5041
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5041
augaaaaaca cucuauaugg aaucggucuc gauauaggug uagcaagugu ugguugggca       60

gugguuggac uuaauggaac aggugagcca guuggauuac acagguuagg uguaagaaua      120

uuugauaaag cagaacaacc aaagacagga gaaaguuuag cagcaccaag aagaauggca      180

agaggcauga gacgaagguu gagaagaaaa gcguuaagac gagcggaugu uuacgccuua      240

cuagaacgau caggacuaag cacgagagaa gcauuagcac aaauguuuga ggcgggugga      300

cucgaagaca uuuaugcucu aagaacaaga gcauuagaug agccaguagg aaaggccgaa      360

uuuucaagaa uacuuuugca ucuagcacaa agaagagggu uuaagagcaa uagaagaacc      420

gcuuccgaug gagaagaugg aagguuacua gcagcaguga acgaaaauag gagacgaaug      480

gcacaagggg gauggcgcac aguaggagaa augcuauauc gacacgaagc auuugcauua      540

cacaaaagaa acaaagcuga cgauuaucua ucaacuguag gaagagauau gguagcugaa      600

gaggcuucau uauuauuuca aagacaaaga gaacuggggu gugcaugggc aacaccagaa      660

cuacaggcgg aauaccuaag uauucuacua aggcagaggu cauuugauga aggucccggu      720

ggagauucac cauauggugg aaaucaagua gagaaaaugg uugguagaug cacuuucgaa      780

ccugacgaac cuagagcagc aaaagcagca uauaguuuug aauauuucuc uuuauuacag      840

aagauaaacc auauaagacu ugccgaaaau ggugaaacua gaccucucac acaaccucaa      900

agacagcaau uauuauccuu agcacauaaa acuccugaua ugucacuagc aagaauuaga      960

aaagaacuag cuuugccgga gacaguacaa uucaacggag uaagaugccg agcuaaugaa     1020

acacucgagg aaucugagaa gaaagaaaaa uucgcauguu uacccgcaua ucauaaaaug     1080

aggaaagccu uagguggugu aguaaaaggc aggauaucuu cccucuccau cagucagagg     1140

gaugcagcug ccacggcuuu aaguuuguau aaaaaugaag acacacugag ggcuaaguua     1200

acagaagcag gauuucaagc acccgaaauu gaugcacuag caggguuaac agguuuuuca     1260

aaauuuggcc auuuaucuuu gaaagcaugc cgaaaacuga uaccacaucu agaacaagga     1320

cuaacauaug aucaggcuug cucggcagca ggauacgacu uuaaagguca cggagcgggu     1380

gaaagagcau uuacauuacc agcagcggcu cccgaaaugg aacaaauuac cucuccggua     1440

gugagacgag ccguagcaca aacaauaaaa guaguuaaug guauaauaag agaaauggau     1500

gcuaguccag cuuggguaag aauagaauua gcuagagaau uaucuaaaac uuuuggagaa     1560

agacaagaaa uggaccgcuc uaugagagaa aaugcagcca aaaaugaaag acugaugcaa     1620

gaacuuagag auacauucca ucuucuuucu ccaacaggcc aagaccuagu aaaauacagg     1680

cuuuggaaag aacaagacgg cguaugcgca uacagccuuc aacgauuaga uguugaacag     1740

cuuuuugaac caggauacgu agacguagac cauauaguac ccuauaguuu gagcuuugau     1800

gauagaagau caaauaaagu auuaguauua aguucagaga aucgacaaaa aggaaauaga     1860

uuaccauugc aguaucuaca aggaaaaaga cgagaagauu ucauagucug gacaaacucu     1920

aguguacaug acuacaggaa aagacaaaac cuauuaagag agaaauuauc agaugaugaa     1980

gcggaaggau uuagacaaag aaaucuacaa gauacccaac auauggccag auuucuguau     2040

aauuauauaa gcgaccaucu agcuuuugca caauccgagg cccuaggaaa gaaacgagua     2100

uuugcaguau ccggagccgu gacaagucau uuacgaaaaa gauggggacu cucaaaagua     2160

cgagcugaug gagacuuaca ucaugcauug gaugcagcug uaguugcgug uacaacugau     2220

gguaugauua gacguauaag cggguauuau ggacacauag aaggagaaua uuuccaagac     2280

gcagacggag cugguucaca gcaugcccgu acaaaagaaa gauucccugc accuuggcca     2340

agauuuagag augaacuaau aguuagacuc ucggaacagc ccggugaacc acuacucgac     2400

auaaauccag cauuuuacug ugaauaugga acagaacaua uaagaccagu uuuuguauca     2460

agaaugccaa gaagaaaagu aacgggucca agccauaaag agacaguaaa aggagcagca     2520

gcagccgaug aaggacucuu aacagucaga aaggcuuuga cagaacuaaa auuagauaaa     2580

gacggagaga uaaaagacua uuauaugccu agcagugaua cuuuacuaua cgaagcccuc     2640

aaagcucaau uacguagauu uggaggagau gggaagaaag ccuucgcaga acccuuuuac     2700

aaacccaaag cugauggaac accuggaccg uuagucagga aagucaaaac uacugaaaaa     2760

gcaaccuuaa caguaccagu acacggugga gcugcaucaa acgauacuau gguuagaaua     2820

gauguauucu uuaugccagg cgacggauau uauuggguuc cacuauacgu cgcagauaca     2880

cuuaaaccug aacuacccaa ucgagccgua guagcacaca aaccauauuc ugaauggcag     2940

gaaaugcgug aagaagauuu cguauuuagu cuauauccaa augauuuagu guacguagaa     3000

cauaaaagcg aauuaaaauu uacaguacaa aacgcagaca guacauuaga gaaaacuaga     3060

acgcaagcaa gugcauucgu guauuuugug uccgguggaa uuucaggugg cacaauaaca     3120

guaagaacuc augacaauuc cuacggaaua ccuagucuag gccuaaaaac auugaaauca     3180

uuaugcaaau accaaguaga uguauuaggu aaugucuucc cgguucauaa agaaacucgu     3240

caaaaauucc gauga                                                      3255


<210>  5042
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5042
augaaaaaca cgcuauaugg aauagggcuc gauauaggag uagcaucagu cggcugggca       60

gucguagguc ucaauggaac aggcgagccu guugguuuac aucgccuagg agugagaaua      120

uucgauaaag cagaacaacc aaagacagga gaaucauuag cagcacccag aagaauggcc      180

agagguauga gaagaagauu acgucgaaag gccuuacgac gugcagaugu uuacgcuuua      240

cuugaaaggu cgggacuauc aacacgugaa gaauuagcgc aaauguuuga agcaggcggc      300

uuagaagaca ucuacgcacu aagaacgcga gcauuagacg aacccguugg aaaagcagaa      360

uuuagcagaa uacuuuuaca uuuagcacaa agaagaggau uuaaaucaaa cagaagaaca      420

gcaagugaug gagaagacgg gaggcuacua gcagcaguaa augagaauag aagaagaaug      480

gcacaaggag gauggagaac aguaggagaa auguuauaca gacaugaagc auucgcauua      540

cauaaaagaa acaaagcuga ugacuaccua aguaccguag guagagauau gguagcugaa      600

gaagcaagua uucuauucga gagacaaaga gcaauggggu guagcugggc cgcucccgaa      660

cuacaagcug aauaccuauc cauauuacua aggcaaagaa guuuugauga aggaccugga      720

ggcaauagcc ccuauggagg aaaucagguu gagaaaaugg uuggaagaug uacauucgaa      780

cccgacgaac ccagagcagc aaaagcagcg uacagcuucg aauauuuuuc ccuuuuacaa      840

aaacuaaauc auauuagacu agcagaaaau ggcgaaacaa gaccacugac ccaaccacag      900

agacaacaau uauuaagucu cgcucauaaa accccggaca uaagucuagc acgaauaaga      960

aaagaguuag cccuaccuga aacuguacaa uucaauggag uacgauguag agcaaaugag     1020

acacuugaag aaagcgagaa aaaggaaaaa uuugcaugcc uaccugccua ccacaaaaug     1080

cgaaaagcau uggauggugu uguaaaaggc agaauuucga guuuaucaau aagucaacgg     1140

gacgcugccg ccacagcacu auccuuguac aaaaaugaag auacauuaag ggcgaaacuu     1200

accgaagcag gauuccaagc uccugaaauu gaugcacuag caggacugac cgguuuuuca     1260

aaauucgguc aucuuaguuu aaaggcuugu aggaaacuaa ucccacauuu agaacaaggu     1320

cugacauaug accaagcaug uacagcggca ggcuaugauu uuaaaggaca uggcgcaggg     1380

gaaagagcuu ucacuuuacc ggcggcagca ccagaaaugg aacaaaucac aagcccagua     1440

gugcgacgag caguugcaca aacaauuaaa guuguaaacg gaauaauacg agaaauggau     1500

gcaaguccag cauggguacg aauagaguua gcacgagaac uuucaaaaac uuucggagaa     1560

cggcaagaaa uggacagauc aaugagagaa aacgcagcgc aaaaugaaag guuaaugcaa     1620

gagcuaagag acacuuuuca uuuacuguca ccaacggguc aagaucuugu aaaauauaga     1680

cuauggaaag aacaagacgg aguaugugca uauucauuaa gaagauugga uguagaacga     1740

uuauucgagc caggauaugu cgacguagac cauauaguac cauauagucu aucuuucgac     1800

gaucgaagaa guaauaaagu uuuaguacug ucaagugaaa auaggcaaaa aggaaauaga     1860

uuaccauugc aauaccuaca agggaaaaga agagaggacu uuauuguaug gacaaauuca     1920

ucaguacacg auuaucguaa acgucaaaau uuacuaagag aaaaacuguc aggugacgag     1980

gcagaaggau uuagacaaag aaauuuacaa gauacacaac acauggcccg auuccuuuau     2040

aacuauauca gcgaccacuu agcuuuugca caaucagaag cacucgggaa gaaacgagug     2100

uuugcagugu cgggagcagu aaccagucau cuaagaaaaa gauggggauu aucaaaagug     2160

agagcagaug gugauuugca ucacgcauua gaugcugcag ugguggcaug uaccacugau     2220

gguaugauua gaaggauaag uggauauuau ggacacauag aaggagaaua ucuacaagac     2280

gcagauggcg cagguagcca acaugcaaga acaaaagaga gauuuccagc accauggccu     2340

agauucagag augaauuaau aguaaggcua ucagaacaac ccgcagaacc auuacuagau     2400

auaaacccag aguucuauug cgaauaugga acagaacaca uacggccagu uuucguauca     2460

agaaugccac gaagaaaagu aacaggaccu ggucauaaag aaacaauaaa aggcgcugcg     2520

gccgcagacg agggauuguu aacaguaaga aaagcauuga cagaacuaaa auuagauaaa     2580

gauggagaaa uuaaagauua cuauaugccu ucaucugaua cacuacuuua ugaagcucug     2640

aaagcucaac uucgaagguu ugggggagac gguaagaaag cauuugcaga accauuuuau     2700

aaaccaaaag cagaugguac cccaggacca cugguccgaa aaguaaaaac aacagagaaa     2760

gcaacgcuua caguccccgu acauggaggg gcagcaucaa acgacacgau ggucagggua     2820

gauguauuuu uagugccggg cgaugguuau uauuggguac caguauauac agccgacaca     2880

cuuaaaccag agcucccaaa ucgugcagua guucaaggua aaccuuauag ugaauggaaa     2940

gaaaugcgag aagaagauuu uauauuuagu uuauauccca augaccuagu uuacguagaa     3000

cauaaaagcg guuuaaaauu caccuugcaa aaugcggacu caacauuaga gaaaacaaga     3060

acacaaacau cagcguuugc guauuuuguu ucaggaucca uaagcacggc aucaauaaca     3120

auucguacac augauaaugc auauggaaua ccuucauuag gaguuaaaac ucuaaaaacc     3180

uuucauaaau accaaguuga uguacuagga aauguguuuc caguacauaa agaaacacgc     3240

caaaaauuca gauga                                                      3255


<210>  5043
<211>  3255
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5043
augagacucg gacucgacau aggaacaaau ucaauaggcu gguggcuaua ugguacagaa       60

ggagguagaa uaacagaagu uguagacggu ggaguaagaa uauucucaga uggaagagau      120

ccccaaagca aagcaagucu agcaguugac cggagagcag cuagaucgca aaggcggcga      180

agggaccgau auuuaaggag aaaggcggca cucaugaaaa gaauggcaca aguagguuug      240

augccagcag auccagcaga agcaaaagua cucgaaguuc uuaacccaua ugaauugaga      300

gccagcggau uagacggacc acuaccacua acacauuuug guagagcucu uuuccauuua      360

aaccaacgga gaggguuuaa aagcaaccga aaaaccgaua gaggagauaa ugaaucagga      420

aaaauuaagg augccacagc acgacuugau caagcaauga uggcaaaggg cgcaagaaca      480

uacggugaau uucuacauau gaggagagca gccgcuuuag auccuaaauc aguuccuagu      540

guuagaacac gguugacugu agcaaggagg gacgacgcug agaaagaaga agcaggguau      600

gauuucuauc cggacaggaa acauuuaucg gaagaauuug auaaauuaug ggccgcgcaa      660

gcucgccacg cuccagacgu ucuuacagaa gauuuacgcg acgaaauugc aacaauaauc      720

uuucaccaac gaccacuuaa aacaccagaa guaggucuau gucuauuuac aggacaacac      780

ggaguaccug aacgagacaa aagaauaccg agugcacauc cuuuaaauca aagacgaauc      840

cucauggaaa caguuaacaa cuuaaaagua gcagcacaag gagaaccuag cagaccacua      900

acaagagaag aaagggauca aauuguacac gcacuagaca acaaaaagca cacaaaaagu      960

cuuucaggaa ugacuaugaa acuaaaagca uugggaaagg uuauuaaacu aaggcccgaa     1020

caaggauuua cacucgagac ugcuaacaga gacgcaauag cuugcgaccc aguaagagca     1080

aguuuaaguc aucccgacag auucggggcg agauggagua cauuagaauc ugcaggucag     1140

ugggauguag uacaaagaau aagagcggua caaagugaug cugaacaugc agauuuaguc     1200

gguugguuaa uggaugugca cgguuuagau cgagaucacg cggaagaaac cgcaaaugca     1260

ccucuaccag aagguuacgg uagauuaggc cugacagcua cuacuagaau acuagccgcc     1320

cuagaagccg augugcugaa cuauucagcu gcaguggcag caugcggaug gcaucauagu     1380

gaugguagaa cgggagaagu uuuggaauca cuaccuuacu acggggaaau acuagaaaga     1440

cauguaauuc caggaacugg agaucccaau gaugaugaug ugaaaagaua cggucgaauc     1500

acaaauccua cuguacauau aggguuaaau caacuaagaa gacuuguaaa ucgaauaaua     1560

acaguauacg gacgaccuga ugaaauagug gucgaacuag cuagagaucu aaaacucagu     1620

gaagaucaaa agaaagaugu ccaaagggau aucaagaaaa acacagaagc ugcaauaaga     1680

cgcggaaaga aauuagaaga aaugggaguu ccaaacaccg gagccaacag aaugguacua     1740

agguuauggg aagacuuggg agaugacguu augacaagaa auugcccaua uacaggcaaa     1800

aggauaagcg caggaauguu auuugacgga agcugugacg uggaucauau ucuaccguac     1860

ucaagaaccc uagaugauag uuuugcuaau aggacccucu gucuaaaaga agccaauagg     1920

cagaaaacug auaaaacacc augggaggcc uggggagaaa cagagcaaug ggaagcaauc     1980

gcagcaaacc ucaaaaaucu accagccaau aaagccugga gguuugcacc agacgcaaug     2040

gaaagauucg agggugaaaa agauuuuuca gcaagagcgc uagccgacac ucaauauuua     2100

agucgaauug ccagggcaua ucuagacgca uuauaugaug gagaugaugg aaaaucccau     2160

gucuggguag uaccugguag acuaacagaa augcucagga ggcauugggg ucuaaacgga     2220

guugcugcuu uaacagauag cgauaaacaa acagucaaag cuaaaaaucg aacagaccac     2280

agacaucaug caauugacgc ugcaguagua gccgcaacug auagaggacu aauuaaaaga     2340

auagcagaua uggccaaaag agaugaguuu aauggagcag aagaagucgc ccggaguaua     2400

ccaccaccuu gggaaggauu ucgugacgau guuucggcac aaguaacuag aauaauagua     2460

agccacagag cagaucaugg aagaauugac ccggcagcca gaaaaucagg cagagauagu     2520

acaucuggcc aauuacauaa cgauacagcu uaugggauag guagagacgg cacaguggua     2580

uccagaaaac cauuagcauc cuuaucgcca aaugacauag uuguaacaaa acguggcgcu     2640

aauauacggg acguugaccu gcaaaagcau uuagcaagag uuacccacgg uuuagaugga     2700

aaggcauuug aagcugcucu uuuagccuuu gcagcaagcc caauauuacc ugauaacacu     2760

gauaauccau acuucgguuu acggcgggug cgacuagaag agacguugca agaaucugcu     2820

agaguagaag uagccgauca agaaggaagg cccuacaaag cauauaaagg agauagcaac     2880

cauugcuaug aaauauggug ccuaccagau ggaaaaauaa aaccacaggu uguuacuacc     2940

uacgaagcuc accaaagcgg cgcagaaaag aaaccccacc cagcagcaaa aaggcuuaug     3000

agaauuuuua aacgagauau gguuaugcua gaaagggacg gagaaacaau aauugguuau     3060

guacagaaaa ugaaacaaaa uggcagcaua uuuguggcac cucacacaga agcuaaugca     3120

gacgcacgag acagggaucc aaaagaugac uuuaaauuaa uacaauuagg cgcggguucu     3180

cuuguaaaag caaaagcaag acgaguaaua guggacgaaa ugggacgacu aagagaccca     3240

ggaccacccu uauga                                                      3255


<210>  5044
<211>  3258
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5044
auggauaaag ggaccaacag cauagguugg gcugcucuga gguuagauga acaaggagaa       60

ccggugggga uaauagaagc uggaguuagg aucuaugcag auggaagaga accaaagucc      120

ggaacaucgu uagcagaggg ucgaagaaua gcaagaggga ugucaaggag aagagaucga      180

uacaaaagac guagaagaac uguauuaaau acauuaacag aguucggauu gaugccagca      240

acaaaagcag caaggaaggc auuaguggca gaaacaaaug accgaagauu cggagaagua      300

gccaguaaug ucuaugcauu gagagcacga gcucuagaug aaaaauuacc ucuauaccaa      360

cuagguagag cuuuauuuca ucuaaaccaa cgaagaggau uuaaaagcaa uagaaaaaca      420

gauaaacggg auaaugauca aggaaaaaua gcaucagcaa ucagcagacu agguagaaaa      480

uuagaugaag caggcgcuag gaccuuagga gaguuccuac auaaacagca ugccgcuggu      540

aaauggguac gaguacgccc aaguagagua ccaggagaag augacaagga agaagaugga      600

uauggauucu acccugaaag gucauuuuug gaacgagaau uugcagcuau uugggaagca      660

caagcggcuu uuuacccuga gcuuuuaacg gaggaaagaa gagaucaucu acacuggguc      720

auguucuuuc aaaggccguu aaaaagacca acgguaggaa aaugcucguu ucacccagac      780

gaggaucgac uuccaaaagc acacccacuu uuucaagaau uucgacuaau aaaagaguua      840

aacgaacucg aaguagucca uagaggugau agaagggaaa aacuuacacc agaucaaaga      900

gacagguuau uuaaccaauu aagaacacaa agauccagug cauuuacggc auuacguaaa      960

acaauaaaag caccaacagg uacgagauuu aauaaagaaa gugaugcaag agauaaacuu     1020

aaaggagaug aaguugcugc agaaauguca cauaaggaua gguuugguca agcguggaua     1080

ggcaugccau uagcggaaag augggcaguu guugcgaugu uauuggauac agaagauccg     1140

uuagaaguaa aagcauggcu ugaagaaaga uacggguucg cagcagacag gauagaugcu     1200

aucgcugcag cuaaauugcc aacaggcuac ggaagacucg gagaaacagc acuacgugau     1260

auguuagacg aauuaagaaa cggaacagug aaagacgacg ccggacggga uguuguacuu     1320

acggaacgac aagccaugga uaacauaugg ggugauaacc cagcauuaca aagagcagua     1380

acagaugcga gggagcuucc gcccuaccaa gaaguacuag gccgaaggau accgccugga     1440

accggaaaac ccgcugaccc uuaugacguc caaaaaggaa gacugacaaa uccuacagua     1500

cauauaggac uaaaccaguu gaaacgaaua guaaauguau uaaugagaag acacggaagg     1560

ccugaacaga uagcuuuaga auuagcuaga gaccugaaac ucaaugaaga acaaaaagcg     1620

gagaaaaauc gcgaaauagc caggaacacu aaagcagcag aagcuagggg aaagaaauua     1680

gaggaacuug ggcacgcaaa uaauggauau aaucguguac uacuaaaacu augggaagac     1740

uuaaauuuaa aacaaccaga aaacagggaa uguauauacu gugguaaagc aaucggaauu     1800

gggacacuuu uaucaaacca aguugauauu gaccauauau uaccauggag caaaacccua     1860

gaugacucuc aagcaaauag aauaguuugu cauguagaau guaauagaca aaaagguaac     1920

cacgcaccag cggaaguucc acaauggcau gauagauaug acgauauuuu agcaagggca     1980

gaacgauuac caagaaauaa agcauggcga uuuggacgug aggcaaugaa gaaauuuaaa     2040

gaugaugaau cuuucuuagc aagacaacuu acagauacuc aguauuuauc aagauuggcc     2100

caugaauauu uaggagcguu guacccugau gaggaaccgg augagugggg uggaccagcc     2160

cguagaaauc augucagggu uauacccgga cguaugacug aaaugcuaag aagacaaugg     2220

gguuuaaaug caauaauuuc cgaccauaau cagaaaaaca gaaaugauca uaggcaucac     2280

gcaauugacg caauuguugu aggaguaaca acacgaagau uacuacaacg aaucgcaacg     2340

guagcaggag gacgagaggc ucuagcguua gaagagacaa uaggaaaaau agaaccucca     2400

uggccagguu ucuaugacga uguaaaagca gcuaucgaca gaauagucgu aucucauaaa     2460

ccagaucacg guucauuacc aaaacccgga aaaccaggua gaacagcggg acaacuccau     2520

aaugauacag cauauggauu uacuggugaa gcacguaaug gcgugccgau uaugguacac     2580

cguguaccau ucgcaucuuu gacagaaaaa gauauaccau cgauaagaga uagacaauua     2640

gcagaugcac uuuaugcggc uauaucagga auaucaggca aggcagaauu ggcugcagcu     2700

cuggcaagau ucagugaauc uggcccauau aaagggauuc gucaugugcg aguuguugag     2760

cccgucucag ucauugcaau uaaagaccaa gcaggaaaag cguacaaagg auauaaaggu     2820

gaugcaaauu acagauauga uguaugggaa cuaccuaaug gaaaguggac gcacgaaguu     2880

gugaguaugu ucaaugcgca ucgaccagac uggcaaucag aaauacgaag uaaaaacccu     2940

gcagcaagaa aaguucuauc auuacaucaa aacgacaugg uugcguacga acacccacaa     3000

gacgguuaua caauagggag aguaguaaaa uuuucugcug gaggacaaau ggcauuggca     3060

ggaaauagag aagggggagc ucuaaaaagc agagaugcug auccagaaga uagguucaga     3120

uauuuuuaua aaucaggggg agcaaugaga gacguacaau ugcgacaagu uagagucgau     3180

gaaacaggaa gaguuuuuga uccuggaccu caagauagag agucgaggaa ugcaagaaaa     3240

caaaacgacg caggauga                                                   3258


<210>  5045
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5045
augcgguaca aaauaggauu ggauauagga auaacaagcg uaggcugggc agugaugaau       60

uuagacaucc cuaggauaga agauuuagga guuagaauau uugacagagc agaaaacccc      120

caaacaggag aaucauuagc auuaccacga agacuggcaa gauccgcgag acgacgccua      180

agacgaagaa aacauaggcu agaacgaaua cgacgacuug uuauaagaga agguauacua      240

acaaaagaag aauuagauaa auuauuugaa gaaaaacacg aaauagacgu auggcaacuu      300

agaguugaag cacuagaccg aaaacuaaac aacgaugagu uagccagagu ccuacuacac      360

uuagcuaaaa gaagagguuu caaaaguaau aggaaaagug agagaagcaa caaagaaaau      420

aguacaaugc uaaaacauau agaagaaaac agagccauau uaucaucaua caggacugua      480

ggugaaauga uaguaaaaga uccaaaauuu gcccuacaua aaagaaauaa aggcgaaaac      540

uacacuaaua cgauagcacg cgacgaccug gaaagggaaa ucagacuuau auuuagcaaa      600

caaagagagu ucggaaauau gucgugcaca gaagaguucg aaaacgaaua uauaacaauc      660

ugggccaguc aaagacccgu agcuucaaaa gacgauauag aaaagaaagu aggcuuuugu      720

acauuugaac ccaaagaaaa gagagcaccu aaagccaccu acaccuucca gagcuucaua      780

gcuugggaac auauaaacaa auuacguuua auaucaccuu cuggagcaag aggauuaaca      840

gaugaagaaa gacgacuucu cuaugaacaa gcauuucaaa agaauaaaau uacauaucac      900

gacauaagaa cauuacucca ucuuccugau gauacuuacu uuaaaggaau aguauacgac      960

agaggagaaa gcagaaaaca aaacgaaaau auacgcuucc uagaauuaga ugcauaccac     1020

caaauuagaa aagccguaga uaaagucuau gguaaaggca aaucuuccuc auucuuacca     1080

auugauuuug auacauuugg cuaugccuua acccuauuca aagacgaugc agacauacac     1140

ucauaccuac gaaacgaaua cgaacaaaau ggcaagagaa ugccuaaucu ggcaaauaaa     1200

guauacgaua augaauugau agaagaauua uugaaccuuu ccuuuacuaa auuuggccau     1260

cugucccuga aagcucucag aucgauacua cccuauaugg aacaaggaga aguauacagc     1320

agcgccugcg aacgagcagg guacacauuu acaggcccua agaagaaaca gaaaacaaug     1380

cuacuaccca acauaccucc aauugccaac ccaguaguca ugagagcucu aacacaagcu     1440

cgcaaaguag ucaaugcuau aauuaagaaa uauggcagcc cagugagcau acauauagaa     1500

cuagcacgag aucuaaguca aaccuucgac gaaagaagaa aaacaaagaa agaacaagac     1560

gaaaauagga agaaaaacga aacagcaaua agacaauuaa uggaauacgg ccucacacuc     1620

aacccaaccg gacacgacau aguaaaauuu aaauuaugga gcgaacaaaa uggcagaugu     1680

gcuuauucau uacaacccau cgagauagaa cgauuauuag aaccagguua uguugaagua     1740

gaccacguaa uuccuuauuc acgaucgcua gaugauagcu auacaaauaa aguacuugua     1800

cuaacaagag aaaacagaga aaaagguaau cgaauaccag cagaauauuu aggaguagga     1860

acagaaagau ggcaacaauu cgagacauuu guucuaacaa acaaacaauu uaguaaaaag     1920

aaacgugaca gacucuugcg ucuacacuau gaugaaaacg aagaaacaga auuuaaaaac     1980

agaaaccuua augauacaag auauaucucc agauucuucg caaauuuuau cagagaacac     2040

cuuaaauuug cagaaaguga cgauaaacaa aaagucuaua cuguuaacgg aaggguaacu     2100

gcacaccuuc gaucaagaug ggaguucaac aaaaaccgug aagaaucaga ccugcaucac     2160

gcagucgaug cuguaaucgu ggcauguaca acaccuagcg auauagcgaa aguaacagcc     2220

uuuuaucaaa gacgagaaca aaacaaagaa cuagcgaaaa agaaagaacc acauuuucca     2280

caaccuuggc cacauuuugc agaugaacuc agggcacgac uaucaaaaca uccuaaagaa     2340

aguauaaaag ccuuaaacuu gggcaauuac gaugaucaaa aauuagaauc auugcaacca     2400

guauucguaa guagaaugcc caaaagauca guaacggggg cugcacacca agaaaccuua     2460

agaagguacg uaggcauaga ugaacguuca ggcaaaauuc aaacaguagu uaaaacaaaa     2520

cuaucagaaa uaaaacugga cgcaucagga cacuucccca uguaugggaa agaaagugau     2580

ccaaggaccu augaagccau acggcaaagg cuccuagaac auaauaacga uccgaaaaag     2640

gcuuuucaag aaccuuuaua uaaaccaaag aaaaacggag aaccaggacc aguaauaaga     2700

acaguaaaaa ucauagauac caaaaaucaa guuauaccau uaaaugaugg uaaaacagua     2760

gcuuauaaua guaauauagu uaggguagau guuuucgaga aagacggaaa auacuacugu     2820

guacccguau acacaaugga uauaaugaaa ggaauccuuc caaacaaagc aauagaacca     2880

aacaaaccuu acucagaaug gaaagagaug acagaggauu auacauuuag auucucguua     2940

uauccaaaug aucucauaag aaucgaacua ccacgggaga aaacaguaaa aacagcagca     3000

ggagaagaaa uuaacguaaa agauguauuc guuuacuaua aaacgauaga cuccgcaaac     3060

ggaggccucg aauuaauuuc ucaugaucac cgauuuagcu uacgaggagu gggaucuaga     3120

acacuaaaac gcuuugaaaa auaucaaguc gacgugcuug gaaacauaua caaaguaaga     3180

ggagagaaaa gagucggacu ugcuucuuca gcacauucua aaccaggaaa aacaauaaga     3240

cccuuacaau ccacaagaga uuga                                            3264


<210>  5046
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5046
augcucagaa ugaaauacgc aaucggacuu gauauuggca ucgcuucagu aggcuaugca       60

guguuagccc uagaccacga agagaacccc uggggaauca uuagacuagg aaguagaaua      120

uuugauguug ccgaaaaucc aaaagaugga gcaagucuag cucuaccucg aagagaagca      180

agaagcguga gacgacgucu aagacgacac caucauagac uagaaagaau uaaaaaucua      240

cuaauaaaca ccgaguucau aacaaaagac gaacuguuac aucuuuauga uggaaacuua      300

ucagacguau acgaacucag aguaaaagcu cuagacuauu ccguaacaaa ugcggaacug      360

acaaggauau uacuucaccu agcucaaaga cgaggcuuua aaagcaaucg aaaaagugac      420

gccaaugaca aagaagcagg acaauuacua gaagcagugu ccgcuaaugc aaaacgaaug      480

caagaaaacc auuacagaac cguaggagaa auguuuuaua aagaugacuu auucaguaaa      540

uauaaaagaa acaagggagg aacauauuua acaacaguac aucgagacau gauagcagca      600

gaagcucgaa caauauuaga aaaacaauuc gccuuaggaa auaauauaug uacccaagac      660

uucauagaua aauaucuauc uauacuucua agccaaagac aguuugauga agggccugga      720

gaaccauccc cauaugccgg aaaucagaua gcaaauauga uaggcaaaug uacuuucgaa      780

ccgaacgaau acagagcagc aaaagcaucc uauucguuug aaagguucaa uuuacuacaa      840

aaaguaaauc aucuccgccu auuacuugaa ggaaaaagua uagcacuaga caacgaacaa      900

agaaagaaaa uaauagcucu ugcccacgac aaagcugacc uuagauacuc ccacauaaga      960

aaagcccucg gauuagacga aaaaguacua uuuaacacaa uaacauauaa cgacgacgua     1020

agcguaauag agaaaaagac aaaauucaac uuuuuacagg cuuaucacca aauaaagaaa     1080

guucuagcug aagauaugca aaaauuaaca accgaacaac uggacaauau aggacaaaua     1140

cuaucaacau auaaaaguga caauaaacga accgaagaau uaucggcacu cggccuugag     1200

aagaaaauaa uagacgcauu acuaggaaua aauggacuaa guaaauucgc acaucuauca     1260

cuaaaagcau ugagaaagau aaauccauau cuagaagaag gaaagaucua uaacgaagcg     1320

uguacagccg cuggauauga uuuuaaagcc caugcaaaca cucagaaaac agaacuacuc     1380

cccgcauaca aagaagaaau ggaugacaua accucacccg uagcuaggag ggcgaucgca     1440

caauccauaa aagugauuaa cgcgauaaua agagaacaaa aaggcagucc acuguacaua     1500

aacauagaac uagcaagaga aauggcaaaa ggcuucgacg aaagaaccca aauagacaaa     1560

gcaaacaaag aaaaccaagc caaaaacgaa agaauaaugg aaagaauaag aaccgaguuc     1620

cauaaagcca acccuacagg cauggacuua auuaaacuaa aauuguggga agaacaagac     1680

ggcagaaguc cauacaguca aaaagcaauu aguauaaaca gauuauuuga accaggauau     1740

guugauauag aucacauagu accauacagu aucucauuug acgacucauu uaaaaacaaa     1800

guauuagucu uuucagauga aaaucgagac aaaggaaacc gacuaccuau agcguacuua     1860

caaucaaaau uuggcgcaaa agcggcagaa aauuucauga uauggguuca aagcaacaua     1920

aaagauuaua aaaagagaca aaaacuucuc aaaagagaga uaacagaaga agauauaaau     1980

aaauuuaaag aaaggaaccu ccaagauacc aaaacaauca gcagauucuu auauaauuau     2040

auaaacgauu accuccuauu cgcacccucu gacaccggca aaaagaaacg aguaacagca     2100

guaaacggaa caaucaccgc auaucuacga aaacgauggg ggauaaauaa aaucagggcu     2160

aauggagaua aacaucaugc cguagacgcc guagugauag cuugcacaac agacaaaaug     2220

auuaaagacu uaaguagcuu uagucgauau caugaacuag aguauacgca cacagacacu     2280

gaaaguuucc uuguaaacag uuuaacagga gaaauucuaa aaagguuucc auacccgugg     2340

gaagacuuua gaccugaauu aauggcccgg cuaucagaua auccagcaga cgccuuaaga     2400

aagcugaauu uaauauuuua ucacggaauc gaccuaucaa cgauaaagcc caucuuugua     2460

ucaagaaugc cccgucacaa aguuacggga gccgcccaca aagcuacuau uaaaucagcu     2520

agaugcuuag acaauggcau cgucauaugc aaaacaucuc uacaaaaucu aaaacuagac     2580

aaggaaggcg aaaucgcaaa uuauuaugca cccgaaagcg auacgauucu auauaaugca     2640

uuaaaagaaa gauuaagaag cuacgauaau aaaccagcaa aagcauucgc ugagccauuc     2700

uauaaaccua aagccgaugg aacuccagga cccuuaguaa agaaaguaaa agucuaugaa     2760

aaaucaacuc uuaaugucgc aguacaacaa aacacagcag ucgcagacaa cgauuccaug     2820

gugcgagucg acguauucca cguuaaaggu aacggauacu accuaguacc cauauauaua     2880

gcagacacgc ugaagaaaga acucccaaac aaagcaauag uagcauauaa accauaugcu     2940

gacuggcccg uaauggacga caguaauuuc auauuuagcc uuuaucccaa ugaucuaauu     3000

aaauuugagc acaaaaauag uguaaaauuc gcaaaaguua auaaagaaag cacacuaaau     3060

gauuucuacu ccacaaaauc agaaauagug uauuucaaag guugcaacau aaacacuggu     3120

gcuaucacaa ucauaaauca ugagaauaca uauguuguau caucccuugg agucaaaacc     3180

cuaccaaguc uggaaaaaua ucaagcagau guauuaggaa auuacacaaa aguuaagaaa     3240

gaagucagaa aaccuuuccg auga                                            3264


<210>  5047
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5047
augagauaua aaauaggauu agacaucgga aucacaagug uggggugggc cgucaucaau       60

cuagacauac caagaauaga agaccuagga guaagaauau ucgaucgagc ugaaaaucca      120

caaacagggg aaucacucgc acuuccaaga agacucgccc guucagcacg aagaagacuc      180

agaagacgaa aacaucgauu agaacgaaua cguagauuaa ucauaaggga aggaauacug      240

acaaaagaag aauuagauaa acuauuugaa gaaaaacacg aaauagaugu cuggcaauua      300

agaguagaag cguuagaucg aaaauugaau aacgaugaau uagcaagagu acuauuacac      360

cuagcuaaaa ggagaggauu caaaaguaau agaaaaucag aaagaucuaa caaagaaaac      420

uccacaaugu uaaaacauau agaggaaaac agagcaaucc uuuccucaua uaggacaguc      480

ggagaaauga uagucaaaga cccaaaauuc gcucuacaua aaagaaacaa aggagaaaac      540

uauacaaaca ccauugcccg agaugauuug gagagagaaa uccggcuaau auucaguaaa      600

cagagggagu ucggcaauau gagcuguacc gaggaguucg aaaacgaaua uaucgcaaua      660

ugggcaucac aacgcccugu agcgaguaaa gacgauaucg aaaagaaagu uggauuuugu      720

acauuugaac cuaaagaaaa acgagcacca aaagcaaccu acacuuucca gagcuucauu      780

gcaugggaac acauuaacaa acuccgauua auaagcccaa gcggaacucg agguuuaaca      840

gaugaagaaa ggcgauuacu auacgaacaa gcauuucaga aaaauaaaau aacauaccau      900

gacauaagaa cacuauuaca uuuaccagac gauacauauu uuaaaggaau aguauacgau      960

cgcggugaau caagaaaaca aaaugaaaac auacgauuuu uggaauuaga ugccuaccac     1020

caaaucagga aagcaguaga uaaaguauau ggaaaaggca aauccucauc cuucuuacca     1080

auugauuuug acacauucgg cuacgcucuc acacuauuca aagaugacgc agacauacgu     1140

uccuaucugc gaaacgaaua cgaacaaaau ggcaaaagaa ugccaaaucu agcaaacaaa     1200

guuuacgaca acgaguuaau agaagaauua cuaaaucuuu cuuucacaaa auucggacac     1260

cugagcuuaa aagcauuaag aucaauacuu ccuuacaugg aacaaggaga aguauacuca     1320

uccgcaugcg aaagagccgg cuauacauuu acagggccca aaaagaaaca gaaaacuaug     1380

cugcuuccaa auauuccccc aauugccaau ccaguuguaa ugcgggccuu aacacaagca     1440

agaaaagucg uaaacgcaau aauaaagaaa uacggaaguc caguaaguau acacaucgag     1500

cuagccagag auuuaagcca aacauucgau gaacggagga aaacaaagaa agaacaagau     1560

gaaaacagaa agaaaaauga aacugcaaua agacaacuca uggaauacgg ccuaaccuua     1620

aaccccacug gucaugauau aguuaaauuu aaauuauggu cagaacaaaa cggcagaugu     1680

gcuuacucau uacaaccaau agaaauugag agacucuuag aaccaggaua cacagaagua     1740

gaucauguaa ucccauauuc aaggucauua gacgacucau auacuaauaa aguauuagua     1800

cucaccaaag aaaaccgcga gaaagguaau cgaauacccg cggaauaccu agguguaggc     1860

acagaacgau ggcaacaauu cgagacuuuc guucucacaa acaaacaauu caguaaaaag     1920

aaacgugaca gauuauuaag acugcacuau gacgaaaacg aagaaacuga auuuaaaaau     1980

aggaacuuaa acgauacuag auacaucucu agguuuuucg cuaacuucau uagagaacau     2040

uuaaaauuug cugaaucuga ugacaaacaa aaagucuaca cugugaaugg ccgagucacg     2100

gcccaucuac gcucacgcug ggaauuuaac aaaaauagag aagaaagcga ucuccaucac     2160

gcgguagacg caguaauagu cgcuuguacc acgccaucag auauagcaaa aguuacugcu     2220

uuuuaccaaa gaagggaaca aaacaaagaa cuagcuaaga aaacugaacc acauuuccca     2280

caaccauggc cacacuuugc ugacgaacuc cgagcgagac ucucaaaaca cccgaaagaa     2340

uccaucaaag cucucaaucu aggaaacuau gacgaucaaa aauuagaaag uuuacaacca     2400

guauucguau ccagaaugcc uaaaagauca guaacaggcg ccgcccacca agaaacauua     2460

cgcagauaug uagguauuga ugaaagauca gguaaaauuc aaaccguugu caaaacaaaa     2520

uuauccgaaa ucaaacuaga ugcaucagga cauuuuccaa uguauggaaa agaaucagau     2580

ccuagaacgu augaagccau aagacaaaga cuacuagaac acaauaauga ccccaagaaa     2640

gcauuucaag aaccacuaua caaaccaaag aaaaacggag aaccagggcc uguaaucaga     2700

acagucaaaa uaauagacac caaaaaccaa guuauuccau uaaacgaugg gaaaacagua     2760

gccuauaauu caaauauagu acgagucgau guauucgaaa aagacggaaa auauuacugc     2820

guuccgguau auacaaugga cauaaugaaa ggaaucuuac ccaacaaagc uauugaacca     2880

aauaaaccau acuccgaaug gaaagaaaug acagaagacu acacauuccg auuuagucua     2940

uacccaaacg aucuuauaag aaucgaauua ccaagagaga aaauaaucaa aaccgcaggc     3000

ggggaagaaa uaaaaauaaa agaucuauuc gcauauuaca aaaccauuca cucaggcaca     3060

gcaggccuag aauuaguuag ccaugacugc aguuuuucac ucaguggugu aggcagucgc     3120

acacuaaaac gguucgaaaa auaccaaguc gauguccuag ggaauauuua uaaaguacgc     3180

ggcgaaaaac gcgucggacu ugcaaguagc gcccauucga aaacaggaga aacaauccga     3240

ccacuucaaa gcacuaggga uuga                                            3264


<210>  5048
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5048
augcgauaua aaauaggacu agacauaggu auaacaucug ucggcugggc uguaauuaau       60

cuugauuuac cuagaauaga ggaccuaggu guccguauau uugaccgagc ugaaaacccu      120

caaacgggag aaaguuuagc auuaccaaga agacucgcca gaucugcaag acguagacuu      180

agacgacgua gacacagacu agaaagaaua agaagacucc ucacgaggga aaauguguua      240

acacaagaag aacuugacaa acuauuugaa aaaaaacacg aaauagacgu uuggcaacuu      300

cgcguagaag cauuggauag aaaacuuaau aaugacgaau uagcuagggu gcucuuacau      360

cuagcgaaaa ggagagguuu caaaucaaau agaaagucag aaagaagcaa uaaagaaaac      420

agcacuaugu uaaaacacau agaagagaau cgauccauau uaacaggaua ccguacugug      480

ggagaaauga uaguaaggga cccaaaauuc gugcuacaua aaagaaacaa aggcgagaac      540

uauacuaaua ccauugcaag agacgaccua gaacgagaaa uacaacuaau cuuugcucgc      600

cagcgugaau uugguaauau gauauguacg gaggcauucg aaaacgaaua uauagcaaua      660

uggaguaguc aaagacccgu cgcaucuaaa gaugauauag aaaaaaaagu aggauuuugc      720

acuuuugaac caaaagaaaa acgagcuccc aaagcaaccu acacauuuca aucauuuaua      780

gcaugggaac acaucaauaa acuacgacua guaaguccaa gcggcacacg cgaacuaacc      840

gacgaagaaa gacgguuacu uuaugagcaa gcguuccaaa aaaauaaacu aaccuaccac      900

gacaucagaa cccuccuaca ucuaccagac gacacauacu ucaaagguau cguguacgau      960

uggggagaac cucugaagca aaacgaaaau auaagauucu uagaauugga ugcauaucac     1020

cagauacgua aaacaguaga caaaguguac ggccgcggaa aaucuagcuc auuucuaccu     1080

auagauuuug auacuuucgg auacgcacua acuuuauuua aagacgaugc acauauacgu     1140

ucauaccuaa ggaaugaaua ugagcaaaac ggcaaaagaa uagcaaaccu ggccaacaaa     1200

guauacgaca augaacuaau agaagaacuc cuaaaucuau cauuuacaaa auucggccau     1260

uuaaguuuga aagcacuaag aaacauauug ccauauaugg agcaaggcga aguauauagu     1320

ucugcaugcg aaagagcagg auauaccuuc acaggaccua cuaaaaaaca aaaaacuguc     1380

cuucuaccua caauaccacc caucgcaaac ccggucguaa ugcgcgcacu aacacaagca     1440

agaaaagugg uaaaugcaau aaucaaaaaa uaugguucuc caguaaguau ccauauagaa     1500

uuagcacgug acuuaucaca aacauucgau gaaaggaaaa aaacaaaaaa agaacaagac     1560

gagaauagga aaagaaauga aacagcgaua agacaacuga uagaauaugg acuaacacgc     1620

aauccaacag gacacgauau cguaaaauuu aaacuaugga gcgaacaaaa uggaaggugu     1680

gcuuauucac uacaacccau ugaaauaaaa agacuauuag aaccaggcua cguggagguu     1740

gaucacguaa uaccuuauuc acgaucacug gaugauucau acaccaauaa agugcucgua     1800

cuuacaaaag aaaacagaga aaaaggaaac cguauaccug cagaguaucu aggagugggc     1860

acagaaagau ggcaacaauu cgaaacauuu guccugacaa acaaacaauu caguaagaaa     1920

aaacgagaua gauuacuacg guuacacuau gaugaaaacg aagaagcuga auggaaaaac     1980

agaagccuca acgacacuag guacauauca agauuuuuug cuaauuucau aagggaacac     2040

uuaaaauuug cugauucaga cgacaaacaa aagguauaca cuguuaacgg cggagucaca     2100

gcacaccuac gauccagaug ggacuucaau aaaaacaggg aagaaagcga uuuacaucac     2160

gccguagaug cugcuauagu agcuugcaca acgcuaucug auauugcaag agugacggca     2220

uucuaccaac gaagagaaca agcuaaagag cuagcgaaaa aaacagaacc acacuuucca     2280

caaccauggc cacacuucgc agacgagcua agggcacggu uaucgaaaaa cccaaaagag     2340

ucaauaaaag cacuaaaccu aggcaauuac gacaacgaaa aacuugaauc cuugcaacca     2400

guguuuguaa guagaaugcc aaaaagaagc guaacaggag cagcucacca agaaacucua     2460

aggagguaca uuggaauaga ugaaagaagu gguaaaauuc aaacgguugu aaaaacuaaa     2520

uuaagugaaa ucaagcuaga ugcaucugga cauuucccaa uguauggaaa agaauccgau     2580

ccaagaaccu augaagcaau aagauuacgc cuacuagaac acaacaacga ucccagaaaa     2640

gcauuccagg aaccauuaua uaagccaaaa aaaaacggag aacuuggacc uauaauucgu     2700

acaguaaaaa uaauugauac aaaaaaucaa guaauacccu uaaacgaugg caaaacgguu     2760

gcauauaaca gcaauauagu gcgcguagau guguucgaaa aagaugggaa auacuacugu     2820

gugcccguau auacaaugga cauuaugaaa aaaguccugc ccaauaaagc aauagaacca     2880

aauaaaccau acucggaaug gaaagaaaug acggaagacu acacauuccg auuuucauua     2940

uacccuaaug auuuaauaag aauagaauua ccaagagaaa aaacaguaaa aacaacagca     3000

gggcaugaaa uaaauauuaa agacguauuu guuuauuaca aaacgaucga cucagcaaca     3060

ggaggccuag aacucauuag ucacgacaau ucguucagcu uaagagguau cggcucccgc     3120

acauuaaaac gauucgaaaa auaccagguc gauguauuag gaaauaucua caaaguccga     3180

ggugaaaaaa gagugggacu ugcgucuucc gcacauccgg agacaagaga aacuauuaga     3240

ccauuacaau ccacacgaga uuga                                            3264


<210>  5049
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5049
augaaaaaac uaguccuagg ccuggauaua gguguagcau caguaggcug ggggaucauc       60

gacgcagaaa auggagacgu uguagacaca ggagucaggc uauucaguuc aagagacgcg      120

uccgaaaaug aauccagaag aacaagccga caauccagac gacuuucgcg aagaaaaaaa      180

cacaggaggg aaagaauaaa acaauuacuc cuggcccaug acauggacau augcgacaua      240

guaaacgaaa acccauacga acuccgagua aaaggccuua acgaaaaacu agaaguauca      300

gaaaucuacg uagcacuaac ccaccuggcu aagcgaagag gcgucaguua ucucgacgau      360

gucguggaag aggguggaac aauaaaaagc gaacacuuaa acaaaaauau ggaagaaaua      420

aaauccggca agaugccuug ugaaauacaa cuagaacgau uugaaaaaua uggacaaguu      480

agaggaauga uagaaauaaa ccaagacacu gaagaaucau cauuccuagu aaacauauuc      540

agaacaucag acuauauuaa agaagcuaaa acaauccuag caacacaaca aaaauuucac      600

aaacaaauca ccaaugaauu cauugacgaa uauauccaca uuauggccuc aaaaagagac      660

uacuucauag guccaggaaa cgaaaaaagc cgaacagacu acggagugua cagaacuaac      720

ggcgacacau gggacaacuu auucgaaauu uuaauaggaa aauguucagu auucccagaa      780

gaaacuagag cugcuagaag uaguuaugua gcucaagaau uuaaucugcu aaacgauuua      840

aacaaccuaa aaauaucgaa ucucgaaagu ggaaaacuau cugccaguca aaaacacgac      900

auaguagaaa aaguaaaaaa cucuaaaaaa uucggaacaa aagaucuucu aaaauacauu      960

gccucaguau gcgaaugcaa aauagaagau auagguggcu gccgcguuga caaaaauaga     1020

aaaccaauaa ugcauaccuu ugaaaauuac agaaaaguuc auaacgcacu uaacgaagca     1080

ggcgucgacu uaagacuauc cgaaacagau uacgacgaau ugaugaaaau uaugacccuu     1140

aacagugaga ccuuagaaau aaaaaagcaa uguaaagaaa agcuaccaca acuaacagaa     1200

gauuauuuaa acguguguuu ggucuuaaau aaaaaaggcg ccuucagcgg cuggcacgcg     1260

cuaucuuuga aagcaaugaa auuaaugaug ccugaauuau acggaacguc aaaaaaccaa     1320

augcaaauaa uacacacaau gcaguuaggg aaagccuauc uaaaaccccu caaucaacaa     1380

aaauaccuaa auucagaggc cauauuagag gaaauauaca acccaauugc ugcaagauca     1440

auaaggcaaa caauaaaagu uacaaaugca auacuaaaaa aauauggaca ucuagacagc     1500

guuauaauag aaaugccucg agauuaucaa accagugaag acgaacgaaa aaugauagaa     1560

aaaaugcaaa aagaaaacga aaaagaaaag aaagcggcuc ugaaaagagc gagagaggag     1620

uauccauuuc cagacgaagc auuuagaaau cacaaagaac ucgcuaccaa auuaagacuu     1680

ugguaccaac aggaucaauu cuguguuuau ucuggacgaa aaauuuccgu ugucgaccuc     1740

guaagaaacc caaaucaauu cgaaauugau cacauaauac cucaaagugu auccuuagac     1800

gauaguuuaa acaacaaagu auuaugcuuu agugaagaaa accaaaagaa aggacaacaa     1860

acaccuuuuu auuaccacaa gggcaaaagc gguaacugga cguacgaaca auacaaacua     1920

uucguagaaa aacuauucga uaaaaaaaaa auaaguaaca aaaaaagaga auuacuucua     1980

uucgaagaag acauaaauaa augggaugua agaaggggau uuauagcacg aaacuuaguu     2040

gacacacgau acgcaucaag agugauacuc aacagcuacc aacaauauuu cggaaaucau     2100

gaaaugggaa caaaagucaa agucguuaga gguaaauuua cgucccaacu uagaaaaaaa     2160

uggagaaucu ucaaagauag aaaugaaagc cacucacacc acgcaauuga cgcccuaaua     2220

guagcugcaa cagcuaaccu aagacuaugg aaaaaaaaua cacuaggugu aagcgacgaa     2280

ggcaaaguuu acgauaagga aacaggagaa auuuuaccau ucgaaaaacu aacugaaagu     2340

gaauauaacg aaaugaugaa caaagaacca uaccuaaauu uuaaaaccaa acuucaaaau     2400

auagaaacaa aaguaaaaua uagcuacaga guaucaaaaa aaguaaauag aaaaaucuca     2460

gaugccacaa uuuauaguac acgagaaaga gauggcaucg acuauauagu aggaaaaaua     2520

aaaaacauuu augacguaaa aaccuacaaa gaccaauucc uaaaacgcua caaaaaaaac     2580

aaagagcaau ucuugaugua ucaacaugau ccacaaacuu ucgcaauacu cgaaaauauu     2640

auggcgacau accaggacgc agaaaaccca uucuuagcau acaaaaagga auacgggguu     2700

auaaggaaau acuccaaaaa aggugggggu ucuccaguaa ccucacucaa auuuauagac     2760

aaaguacuag gacaaaaaau agaucucacc gcaaaaaacc aggccgcauu accuaaaaac     2820

aaaaaaguag uauuacaauc aauaacacca uacagagcag acguauacua ugacgaaauu     2880

gacaagagau accaccuaau aggaguuaaa uacucacugu uuuccuuuuc gaaacaaggu     2940

uacguaauca acccuauugc guaccaaaaa uuguuagaga aagaacaaau acacucagag     3000

augaaauuuu guuucagcuu auacagagaa gacauaauag aauuugguga agacccagac     3060

ucccucacaa gauacagguu uuggucuaaa aacgagagcg gcaaaaacaa aauagaagua     3120

aaaccuaucg aaaaaccgaa uuuugagaaa caaacaguac ucaccaucac uaaaaaaauc     3180

aaaaucuuga acaaauacca aacagauguc uuagggaacu cuuuuaaagc ucaagaagaa     3240

aaagacccaa gaaauaucga auga                                            3264


<210>  5050
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5050
augagguaua aaauaggauu agauaucggc auaaccucag uggguugggc uguaaugaac       60

uuagacauac ccagaauaga agaccuaggg guaagaauuu ucgacagggc ugaaaaucca      120

caaaccgggg aaucccuagc acuaccccga agacuagcaa gaucagcacg cagaagacug      180

agacgacgua aacauagauu ggaaagaaua agaagauuag ucauaagaga aggaauacug      240

acuaaagaag aauuagacaa acucuucgaa gaaaaacaug aaaucgacgu auggcaacua      300

aggguugaag cccucgaccg uaaacuuaau aaugaugaac uagcaagagu gcuauuacau      360

cuagcuaaac gacgaggauu caaaaguaac agaaaaagcg aaagaagcaa uaaagaaaac      420

ucaacaaugu uaaaacacau agaagaaaau agagcuauac uuucauccua cagaacugua      480

ggagaaauga uaguaaaaga uccuaaauuc gcuuuacaca aaagaaacaa aggagaaaau      540

uacacaaaua caauagcacg agaugauuua gaaagagaaa uaagacuuau cuucaguaaa      600

caacgagaau uuggaaacau gucuugcaca gaagaguuug aaaaugaaua uauaacuaua      660

ugggccucuc aaagaccagu cgcuucaaaa gaugacauug aaaagaaagu aggauucugu      720

acauucgaac cuaaagagaa acgagcccca aaagcaaccu acaccuucca auccuucaua      780

gcuugggaac acaucaauaa auuacgacua auaucaccga gcggagcuag aggucuaaca      840

gacgaagaac gacgguuacu auacgaacaa gcauuucaga aaaauaaaau aaccuaucac      900

gacaucagga cuuuauuaca cuuacccgac gauacauauu uuaagggaau aguuuacgau      960

agaggcgaau caagaaaaca aaaugaaaac auacgauucc ucgaauuaga cgcguaccac     1020

caaauucgga aagcaguaga uaaaguuuac ggcaaaggua aaagcuccag cuuucuacca     1080

auugauuucg acaccuucgg auacgcucuc acacuguuca aagacgacgc cgauauucau     1140

ucuuauuuaa gaaacgaaua ugaacaaaac ggaaaacgaa ugccaaaccu agcaaauaaa     1200

gucuaugaua acgaacuaau agaggaacuu cuaaaucuga guuucacaaa auuuggacac     1260

uuaagucuaa aggcuuuaag aucaauauua ccguacaugg aacaaggaga agucuacucu     1320

ucggcaugug aacgcgccgg auauacauuu acgggcccaa agaagaaaca gaaaacuaug     1380

uuauuaccaa auauaccucc aaucgcuaac ccaguaguca ugagagcccu aacgcaagca     1440

cguaaaguag uaaaugcuau aauuaagaaa uacggcucac caguaucaau ucauauugaa     1500

cucgcacgug aucucucaca aacuuucgac gaaagaagga aaacuaagaa agaacaagac     1560

gaaaacagaa agaaaaauga aacagcaauc cgucaauuaa uggaauaugg auugacucua     1620

aauccuacag gacaugacau aguaaaauuc aaacuauggu ccgagcaaaa uggaagaugu     1680

gcguauuccu ugcaaccgau ugaaauagaa cggcuccuag aaccaggaua cguagaagua     1740

gaucacguca uaccuuacag uagaucuuua gacgacagcu auacaaauaa agugcuuguc     1800

uuaacaagag aaaauagaga aaaagggaac cgaauaccug ccgaauauuu aggaguugga     1860

acugagagau ggcagcaauu cgaaacuuuu guauuaacaa acaaacaguu cucuaaaaag     1920

aaaagagaua gacuacuaag auuacacuac gacgaaaaug aagaaacaga guucaaaaac     1980

agaaauuuga acgauacaag auauauuuca agauucuuug ccaacuuuau uagagaacac     2040

cuaaaauucg cagaaucaga ugauaaacaa aaaguguaca cagucaacgg acgaguaacu     2100

gcccaucuca gauccagaug ggaauuuaau aaaaaccgag aagagagcga ucuucaccac     2160

gcaguggacg ccgcaauagu ggcaugcacc acucccucag acauagcaaa aguaacugcg     2220

uucuaccaac gacgagaaca aaacaaagaa uuagcaaaga aaacggaacc acacuuccca     2280

caacccuggc cccacuuugc ugacgaauua agagcaagac uuucaaaaca uccaaaagaa     2340

agcauaaaag cacuuaaccu uggaaacuac gaugaccaaa aacucgagag cuuacagcca     2400

guauuuguau caaggaugcc caaaagauca guaacuggag cagcacacca agaaacauua     2460

cgaagauaug uaggcauaga cgaacgaagu ggaaaaaucc aaacaguggu aaaaacaaaa     2520

cugucugaaa uaaaacuaga cgcuagcggu cacuucccua uguacgguaa agaaucugac     2580

ccaagaacau acgaagcgau aagacaaaga uuacuagaac acaauaauga ccccaagaaa     2640

gccuuucaag aaccauuaua uaaaccgaag aaaaauggag aaccaggucc aguuauucga     2700

acgguaaaaa uaauagauac uaaaaaucaa gucauuccau uaaaugaugg caaaacagua     2760

gcauauaaua gcaauaucgu aagagucgau guauucgaaa aagacggcaa auauuauugc     2820

guuccaguau acaccaugga cauaaugaaa ggaauacuac caaacaaagc aauugaacca     2880

aauaaaccau auagcgaaug gaaagagaug acagaagacu acaccuuuag auuuucccuc     2940

uaccccaaug accuuauaag aaucgaauua ccacgagaga aaacagucaa aacagcagcc     3000

ggugaagaaa ucaacguaaa agauguuuuc guauauuaca aaacuauaga uucagcaaac     3060

ggaggauuag aacuaaucuc ccacgaccau agauucucac uaagaggagu aggaucuaga     3120

acgcuaaaaa gauuugaaaa auaucaagua gauguccucg guaacauuua caaaguacgc     3180

ggagagaaaa ggguaggacu ugcaucuuca gcacacagca aaaccgguga aacagugaga     3240

ccccuacaau ccacacgcga cuga                                            3264


<210>  5051
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5051
augagauaua aaauaggauu agauauaggu auaacaucag uagguugggc agucaugaac       60

cuagacauac ccagaauuga agauuuaggc guaagaauau uugaccgagc agaaaauccc      120

caaacagggg aaucuuuagc cuuacccaga cgauuagcua gauccgccag aagaagacuu      180

cgaagacgaa aacauagacu agaacgaauc aggcgucuag ucauccgaga aggaauacuu      240

acaaaagaag aguuggauaa acuuuucgaa gaaaaacacg aaauagacgu cuggcaauua      300

agagucgaag cucucgauag gaaacugaac aaugacgaau uagcacgagu acuacuacau      360

cuagcaaaaa gacgagguuu uaaaucuaau cguaaaucag aaagauccaa uaaagaaaac      420

aguacaaugc uaaaacacau agaagaaaac cgagcuauac uaucaaguua uagaacggua      480

ggugaaauga uagucaaaga uccaaaauuu gccuuacaca aaagaaacaa aggggaaaau      540

uauacaaaca caaucgcuag agaugaucua gaacgagaaa uaagauugau auucucaaaa      600

caaagagaau uuggaaacau gagcugcaca gaagaauuug aaaacgaaua uauaacuauc      660

ugggccagcc aaagaccagu cgcauccaaa gacgacaucg aaaagaaggu uggauucugc      720

acauucgaac caaaagagaa aagagcaccu aaagcaacuu auaccuuuca aucauuuaua      780

gcuugggaac acauaaacaa auuaaggcuc auaagcccau ccggugcacg cggacucaca      840

gaugaagaac gcagacuacu cuaugaacaa gccuuccaga aaaauaaaau aacauaccac      900

gacaucagaa cguuacuaca cuuaccagau gauaccuauu uuaaaggaau aguuuaugac      960

agaggagaau ccagaaagca aaacgaaaau aucagauuuc ucgaauuaga cgcauaccac     1020

caaauaagaa aagcaguaga caaaguauac ggaaaaggca aaagcagcuc cuuuuuacca     1080

auagacuucg acacguuugg auaugcucua acuuuauuua aagacgacgc agauauacac     1140

ucuuacuugc gaaacgaaua ugaacaaaau ggaaaaagaa ugccaaaucu ggcuaacaaa     1200

guauacgaua acgaauuaau cgaagaacua uuaaaccucu cauucacaaa auuuggacac     1260

cuuagucuua aagcacuuag aucaauauug ccauacaugg aacaaggaga agucuacucu     1320

ucagcaugug aacgagcagg auacacauuc acagguccaa agaagaaaca gaaaacuaug     1380

cuacucccaa acauaccacc gauagcaaac ccaguaguua ugagagcacu aacucaagcu     1440

cgaaaaguag uaaaugcaau aauuaagaaa uacggaagcc caguauccau acauauagaa     1500

cuagcaagag accugagcca aacauucgac gaacgccgaa aaacaaagaa agaacaagau     1560

gaaaauagga agaaaaacga aacagcuauc cgacaauuaa uggaauacgg cuuaacuuua     1620

aauccuaccg gucacgauau agucaaauuc aaacuuuggu ccgaacaaaa uggacgaugc     1680

gcauauuccc uacaaccuau agaaauagaa cgacucuugg aaccagguua uguagaaguu     1740

gaccauguaa ucccauacag cagaucauua gaugacuccu acaccaauaa aguccuagua     1800

uuaacucgcg aaaacagaga aaaaggaaac agaauaccag ccgaauacuu ggguguagga     1860

acugaacgau ggcaacaauu ugaaacauuc guacucacua auaaacaauu cucaaagaag     1920

aaaagagauc gcuuacuucg ccuacauuau gacgaaaaug aggaaaccga auuuaaaaau     1980

cgaaaucuaa augauacaag auacauauca cguuucuuug cuaacuuuau aagagaacac     2040

cuaaaauucg ccgaaagcga cgauaaacaa aaaguauaca caguaaaugg uagagucaca     2100

gcucacuuaa gaagucgaug ggaguucaau aaaaaucgag aagaaaguga ccuacaucac     2160

gcaguagacg caguaauagu cgcuuguacu accccaagug auauagcaaa aguaacagcu     2220

uucuaucaaa gaagagaaca gaauaaagaa cucgcaaaga aaacagaacc ccauuuucca     2280

caaccauggc cgcacuuugc agacgaacua agggcaagac uaucaaaaca uccaaaagaa     2340

ucuauaaaag cacuaaaccu aggaaauuac gacgaucaaa aacuagaauc auuacaacca     2400

guguucgucu caagaaugcc uaaaagaucu gucaccggug ccgcacauca agaaacacua     2460

agaagauacg uaggaauuga cgaaagaagc ggaaaaauuc aaaccguagu uaaaacgaaa     2520

uugagcgaaa uuaaauugga cgcuagcggg cacuuuccca uguacggcaa agaauccgac     2580

ccucgaacuu acgaagcuau uagacaaaga cugcuggaac auaacaauga ccccaagaaa     2640

gcauuucaag aaccacuaua caaaccgaag aaaaauggag aaccaggacc aguaauaaga     2700

acggugaaaa uaauagacac caaaaaucag gucaucccau uaaaugaugg aaaaacagua     2760

gcauauaacu caaauaucgu aagaguagau guauuugaaa aagacggaaa auacuacugc     2820

guaccuguuu auacaaugga uauaaugaaa ggaaucuuac caaacaaagc aaucgagcca     2880

aacaaacccu acucagaaug gaaagaaaug acagaagauu acacuuucag auucagccuc     2940

uauccaaaug accuaauaag aauagagcua cccagggaga aaaccgugaa aacggcagca     3000

ggagaagaaa uuaaugucaa agacgucuuc guuuacuaca aaacaaucga cagcgcaaac     3060

gguggccuag aauuaauauc acacgaucac cgauuuucac uuagaggagu aggaagccgu     3120

acacuaaaac gauuugaaaa auaucaagug gacguacucg gaaacauaua caaaguacgc     3180

ggagagaaaa gaguaggacu agcaucaucu gcucauucua aaccaggaaa aacaauucga     3240

ccucuccaau cuacgaggga uuga                                            3264


<210>  5052
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5052
augcgauaua aaauaggacu cgauauaggu auaacaagcg uagggugggc aguaauaaau       60

uuagacauac cgaggaucga agacuuaggg guacgcauau uugacagagc cgaaaauccg      120

cagaccggcg aaucgcuagc acuuccaagg agacuagcua gaucugcaag acgaagacua      180

cgaagaagaa aacacagacu agaaagaaua cgaaggcuaa uaauacgcga aggaauauua      240

accaaagaag aauuagauaa acucuuugaa gaaaaacaug aaauagaugu guggcaacua      300

agagucgagg cuuuagauag gaaauuaaau aacgaugaac uagcacgagu cuuauuacau      360

uuagccaaaa gaaggggauu uaaaaguaac agaaaaucag aaagaaguaa caaagaaaau      420

uccacuaugc uaaaacauau cgaagaaaau agagccaucu uauccgguua cagaacugua      480

ggugaaauga uaguaaaaga uccaaaauuu gccuuacaua aaagaaauaa aggagaaaau      540

uacacaaaua caauagcaag ggaugaucua gaacaugaga uaagauuaau auucucuaaa      600

caacgcgaau uuggaaacau gucaugcacg gaaaaauucg aaaaugaaua uauuacaaua      660

ugggcaucgc agagaccagu agccaguaaa gaugacauag agaaaaaggu cggauucugu      720

acuuucgaac cgaaggaaaa acgugcgccu aaagcuaccu acacuuuuca gagcuucaua      780

gcaugggaac acauuaauaa gcuaagauua auauucccau caggcgcaag aggacuaaca      840

gacgaagaaa gaaggcuauu auaugaacaa gcauuccaaa agaauaaaau aacguaccau      900

gauaucagaa cgcuacuaca uuuaccagau gacacuuacu ucaaagguau aguauaugau      960

agaggagaau cuagaaaaca aaaugaaaau auaagguucc uagaauuaga cgcauaucau     1020

caaauacgaa aggccgugga caaaguauac ggcaaaggaa aaucaagcag uuuucuucca     1080

aucgauuuug acacauuugg uuaugcauua acucucuuua aagaugacgc ugacauacau     1140

ucuuaccuaa gaaaugaaua cgaacaaaau ggaaaaagga ugccgaauuu agccaauaaa     1200

guauaugaca augaauuaau cgaagaacua uuaaauuuau ccuucacuaa guuuggacau     1260

uuaagucuaa aagcacuaag gaguauucua cccuacaugg aacagggaga gguguacagc     1320

ucagcgugug aaagagcagg auauacguuu accggcccua aaaagaaaca aaagacaaug     1380

cuacuaccaa auauaccacc aauugcaaac ccagugguaa ugagagccuu aacacaagca     1440

aggaaaguag uaaacgcaau aaucaagaaa uaugguagcc caguaagcau acauauagaa     1500

cuagcacgag accuaucaca aaccuuugau gaacgucgua aaacaaagaa agaacaagau     1560

gaaaaucgua agaaaaacga gacugccaua aggcaacuaa uggaauaugg guuaacguug     1620

aacccaacag gacaugauau aguaaaauuc aaacuguggu cagaacaaaa uggacgaugu     1680

gcuuauaguu uacaaccaau cgaaauagaa aggcuauuag agccuggcua uguagaaguc     1740

gaccacguaa uaccauauag caggucacua gaugacaguu acacuaauaa aguccuaguc     1800

cuaacaagag aaaaucgaga aaaaggaaac agaauaccag cugaguaccu gggaguagga     1860

acagaaaggu ggcagcaauu ugaaacauuu gucuugacaa acaaacaauu uucaaagaag     1920

aaaagagaua ggcuauuaag acuacacuac gacgaaaaug aagaaacaga auuuaaaaau     1980

cguaaccuaa augacaccag auauauauca agauucuuug ccaauuucau acgugaacac     2040

cugaaauuug cugagagcga ugacaaacaa aaaguuuaua ccguaaacgg acgaguaaca     2100

agccaccuua ggucaagaug ggauuuuaac aaaaaucgag aagaaucgga ccuucaucac     2160

gcuguggaug cagcaauagu agcauguacu acaccuucag acauagcaaa aguaacagcu     2220

uuuuaucaaa gaagggaaca aaauaaagaa cucgccaaga aaacagaacc acauuuucca     2280

caaccauggc cacauuuugc agacgaacua cgcgcacggc uuaguaaaca cccaaaagaa     2340

ucaauuaaag cacuaaaccu uggaaacuau gaugaccaga aacuagagag ccuccaaccc     2400

guauuugugu caagaaugcc uaaaagaagu gugacuggag ccgcacauca agaaacacua     2460

cgacgauaca uugggaucga cgaacgauca ggcaaaauac aaacaguagu aaaaacuaaa     2520

uuaagugaga uaaaacuaga ugccagcgga cauuuuccaa uguacgguaa agaaucugac     2580

ccaaggaccu acgaagcuau aagacagaga uuacuagaac auaauaauga cccuaagaaa     2640

gcauuucaag aaccgcugua uaaacccaag aaaaauggag aaccuggacc gauaauaaga     2700

acagucaaaa uaaucgauac uaaaaaccaa guaauaccac uuaaugacgg aaaaacaguu     2760

gcguacaauu cuaauauagu gagaguugau guauuugaaa aagacggaaa auauuauugu     2820

guaccaguuu auacuaugga cauaaugaaa gguauacugc caaauaaagc aauugaaccu     2880

aauaaaccau acagcgaaug gaaagaaaug accgaagauu acacauuucg auuuagucua     2940

uaccccaacg accuaauaag aauagaacua ccaagggaga aaacaguuaa aaccaccacc     3000

ggagaagaaa uaaacguaaa agacgucuuu guauauuaua aaaccauaga uucagcaaca     3060

gggggacucg aauuaauaag ucaugauaac cgcuuuaguc uuagaggugu aggauccaga     3120

acauuaaaaa gauucgaaaa auaucaaguc gacguacuag gaaacauaua caaaguaaga     3180

ggagagaaaa gaguaggacu ugcaucaagu gcucacucca aaacagguga aacaauaagg     3240

ccacuccaau ccacuaggga uuga                                            3264


<210>  5053
<211>  3264
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5053
augcgauaua agauaggauu agauauaggc auaacaagug uugggugggc aguaaucaau       60

uuggacauac cgaggauuga agaucugggu guacgcauau ucgauagagc agaaaaucca      120

caaacuggcg aaagucuagc auuaccuaga cgacuagcca gaagcgccag gcgaagauua      180

cgacguagaa aacaccgccu agaaagaaua agaagauugc ugauaagaga aggaguccua      240

acaaaagaag aacuagacaa acuuuucgaa gaaaaacacg aaaucgacgu auggcaacua      300

agaguagaag cauuagacag aaagcuaaau aacgaugagc uagucagagu cuuacuacac      360

cuggcuaaga gacguggauu caaaagcaau aggcaaagug aaagauccaa caaagaaaau      420

agcacaaugc ucaaacauau agaagagaac agggccauac ugucagggua cagaacuguc      480

ggagaaauga uaguaaaaga ucccaaauuc gcauuacaca aaagaaacaa aggagaaaau      540

uacacuaaca ccauagcaag agacgauuua gaacgcgaaa uucgauuaau auucucuaaa      600

caaagagagu ucggaaauau gagcuguacu gaagaguucg aaaacgagua uauagcaauc      660

ugggccucac agagaccugu agcaagcaaa gacgacauag aaaagaaagu aggauucugu      720

acguucgaac ccaaagagaa aagggcgcca aaagccacuu auacauucca aaguuucaua      780

gcaugggaac auauaaauaa acuaagauua auaaguccau caggaacacg aggguuaaca      840

gacgaagaaa gaagacuacu guacgaacaa gcauuucaga aaaacaaaau aacauaucac      900

gacauaagga cacuucuaca ccuaccagac gacacauauu uuaaaggaau cguauaugau      960

uggggagaau cucucaaaca aaaugaaaac auaagauucc uagaauuaga ugcauaucac     1020

caaauacgaa aagcaaucga uaaaguauau ggcaaaggaa agucgucuuc cuucuugccc     1080

auagacuucg acaccuucgg auaugcuuua acccuauuua aagaugacgc ugacaucaga     1140

ucauaucuac gaaaugaaua ugagcaaaau ggcaaaagaa uugcaaaccu cgcaaacaag     1200

guuuaugaca acgaacugau agaagaacuc uuaaaccuau cguuuaccaa auucggucau     1260

cucucccuua aggcuuugag gaauauccua ccuuauaugg aacaaggaga gguuuacuca     1320

agugcaugcg aaaaagcagg auacaccuuc acgggaccga aaaagaaaca gaaaacugua     1380

cuccucccaa ccauuccacc aauaguaaau ccagucguaa ugcgagcacu aacacaagcu     1440

cgaaaagucg ucaacgcuau aauaaagaaa uauggaucac cuguuagcau ucauauagaa     1500

cuagcaagag acuuaagcca aaccuucgau gagagaagaa aaacaaaaag agaacaagac     1560

gagaaccgua aaagaaacga aacagcaaua agacaauuaa uggaguaugg ucuuacuuua     1620

aauccaacug gauuggacau agucaaauuu aaauuauggu cagagcaaaa cggacgaugc     1680

gcauauucgc uccaaccgau cgaaaucgaa agacuacuug aaccagguua uguagaagug     1740

gaucacguca uuccauacuc uagaucauua gacgacucau auacaaauaa aguucuagua     1800

cugacuaaag aaaaccgaga aaaaggcaac aggauuccug cggaauaccu aggaguugga     1860

acugaaagau ggcaacaauu ugaaacauuu guauuaacaa acaaacaauu cuccaaaaag     1920

aaaagagauc gacuacucag acuacauuau gacgaaaaug aagaaaugga auuuaaauca     1980

cgaaaccuca acgacaccag auauauuuca agauucuuug cuaacuuuau aagagaacac     2040

cuaaaauucg cugaaucaga cgacaaacaa aaaguauaua caguuaacgg uagaguaaca     2100

ucacacuuaa gaagcagaug ggauuucaau aaaaauagag aagaaucaga ucuccaccau     2160

gccgucgaug cugcaauagu ggccugcacc accccaucag auauagcaaa gguaacagca     2220

uucuaccaac gccgcgaaca aaacaaagaa cuugcaaaga aaaccgaacc acaauuccca     2280

caaccauggc cucauuucgc cgacgaauua cgagcaagac uuagcaaaca uccaaaagaa     2340

ucgauaaaag cacuaaauuu aggaaacuau gacgaucaaa aacuugaaag ccuacaacca     2400

guguucguau caagaaugcc gaaaagaucu guaacaggag cugcacacca agaaacacua     2460

agaagauaca uagguauaga ugaaagaucu ggaaaaaucc aaacgguugu caaaacaaaa     2520

uuaagugaaa ucaagcuaga cgcaucaggu cacuucccua uguacggaaa agaaagugac     2580

ccuagaacau acgaagcuau aagacagagg uuauuagaac auaacaauga cccgaagaaa     2640

gccuuucaag aaccgcuuua uaaaccaaag aaaaacgggg agccaggacc aauuauaaga     2700

acagucaaaa uaauagacac caaaaaccaa guaauuccac ugaaugacgg aaaaacagug     2760

gcauauaaca guaacauagu aagaauagac guauucgaaa aagacggaaa auacuacugc     2820

guaccaguuu acaccaugga cauaaugaaa ggaguacugc caaacaaagc aauagagcca     2880

aacaaacccu acucugaaug gaaagaaaug acugaggauu acacauuucg auuuagccuu     2940

uacccuaaug aucuuaucag aauagaauug ccaagagaga aaacaguuaa aacagccgca     3000

ggagaagaaa uaaauauuaa agacauguuu guguauuaca aaacaauaga cagcgccaau     3060

ggaggauuag aacucaucuc ccacgacaau ucauuuuccu uaagaggcgu aggaucacga     3120

acgcuuaaaa gauuugaaaa auaucaagua gacguacuag guaauauaua caaaguaaga     3180

ggagagaaaa gaguaggccu agcuucauca gcucauucaa aaaccggaga aacuauaaga     3240

ccacuccagu cuacaagaga uuga                                            3264


<210>  5054
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5054
augccaaaag aauucgaccc acuuaagccc gcaaaauaca gauuggcauu agacauaggg       60

agcaaaagua uaggauucgc uauaauauuu cuaaaagaag augucaacaa acuacuuaaa      120

ccuuacgcaa uagucagaag cgguguuaga auauucgggg acgguaggac uccagcugca      180

ccaggacagc uuggcagcag ucuagcagca gaaagacgag uuaaaaggag cauacgacga      240

caauaugcga gaaaacgaaa aagauuacaa agacugcuuc gaaaccuaaa ggaacuaggu      300

uucuuuccag augacucuaa acugugcaaa ucauuugaag aucuaaaucc auaugaacua      360

cgacauaagg gccuuacuaa agaguuaaaa ccccaugaau uuggaagacu ccuaaugcac      420

auggcuaaaa aucguggauu ccagucaaac agaaaaguag acuuacuagc agacaauaua      480

gacucacaag caaaagcaga aacuagugca augaagagcg caauagcaac acuacagagu      540

cgguuaaacg gagaauuuga aacuuuaggc gcauauuuau acgaacgauu uaaaaaagga      600

gaaacuauaa aaucaaguaa aacgcaaaaa ggccaaaaag cggaauauga auucucaauu      660

ucaagaagaa ugguuaaaca agaauucgau cgacuauggg cugcccaaga acaauucaau      720

ccagaacuuu uuaacgagca ggcuaaaaau accuuaaaag acacuauauu uuuccaaaga      780

aaccucuuac cacaaaaagu cggaaaaugc accuucuugc cagagcauga acgagcaucu      840

aaggcucucc cuucaucaca auacuuucga auaaucucag aacuuaacaa gaucaguuuu      900

accgacgccg aagggcuuau auuaccaaua agccuagaac aaagaaacaa acuacugaaa      960

ucacuucgaa uaaaacaaaa auuaaguuuu gacagaauac gaacggaacu aaauuuagac     1020

ucaacaguau uaauaaaucu ugaacacgcu ucaaaggauu cuaucgaagg agacaaaaca     1080

ggauauuuac uaucuaaaaa agaguacuuu ggaccggccu ggagagacuu aucacuagaa     1140

acacaagacg ccauuguaac ugcacugcuc acccacgaaa acgaagcaga acuuguacag     1200

aaacucgucg augauuucaa cauagcacau gacguugcaa aagcccuugc caaugcuaga     1260

uuagaaucac aauauaugag auuaagcaaa gaagccauag aaagauuaau cccagaauua     1320

gaaaaaggug uggacuccca agauggauac aaaguaccgg ugaaauauca ugaggcaaca     1380

gaggcagccg guuuuggaca ccauagcgca uuacaagaaa auugggcuga uuuuccgacc     1440

cuagaucuaa aacaacuaca uucagacgau aacgacaaau acaccguugc agaacuaccg     1500

uuaccauacu auggaaaagu aauuacacgc caagucgcuu ucggaaccgg agacguaaau     1560

gaucccguug aaaaaagauu uggaaaaauu gcuaauccga caguucauau uuguuuaaac     1620

caaguacgug uacuguuaga ugagauuaua gcuaaauaug guuacccaua ugagguaaau     1680

auugaacuag cacgagaacu aaaacaaaac caugaacaac ggcagagaag uauaaaacaa     1740

caagccgcaa auaaaaaaga aaacgaagaa auccgaagag aaauaaaaca agcguacaaa     1800

guugaaaagg caagaauaau aacagauuca gagauuagcc gggccgauau agaaaaaugg     1860

agauuaugga gaagaaguaa aaguaagcua uguguauauu cagggaaaaa aauaugcgaa     1920

acaaugauuu uaaacaauga aguagagguc gaccauauac uaccuaggag uagaacugga     1980

gacaacucau aucaaaacaa aguacucguu cuaaauaaag caaaucgaau caaaaguaac     2040

agaacuccgu gggaagcaaa ggaacuauuu gaagcacagg gauggucaua cgaagacaua     2100

uuaaauagaa ugcgagcacu cgauaaacac caacuauuca gauucgccga aaauuccaug     2160

gacauauggg auaaaaacua ucgauccuuu gaagcaagag cacuaaauga uaccagauac     2220

auaaguagag uaguaaaaga uuaucuaaaa guaguaugcc auaaagaccg aaucgucguu     2280

agcaauggcg cucacacagc ccucauaaga cgagaauucg gacugaacga ucuguuggga     2340

aaaaaaggag aaaaaaaucg agacgaccac cgacaucacg cuauagaugc augcguuaua     2400

ggugcacuag accgaucaau guugagaaaa uugcaaauag caauggaggu agccgaaaaa     2460

caaaauagcg cacgcaauuu ugucggcuua caagaaccau gguuuaauuu cagcacacaa     2520

guacgcaggu caauaggaca aauauaugua ucccauaaac cgaaccauaa uuaugaagga     2580

gagauuuuug aagcaucaau auacggacua aggcucauau ccgauaagag aacagaaaag     2640

uacguaggaa uaaaacgaaa uccagaaaca ggaaaaacug auaaaccuaa aaaauccgua     2700

guaguuauau cagaucccaa aacacaaaac agauauggac uacgccauaa auuucacucu     2760

gaaggacuac caaaaaaaua cuauggacuc ucaucaaguu caguauucug ccucgaaauu     2820

uacagagacg aaaaaggaaa cuggcaagcu uacacuauac cacgguauga agcauacaga     2880

aucguaaaaa gacgaggcgu aaacgcauua uaugacaaua aaagaucuaa cuuugaggga     2940

cgaccgcuag uuaugcgucu aaugcgagau gauugcauag cauucgaaaa aaauggaguc     3000

aaagaaguau auagaauaca gaaaauaaau ggcuuaaaaa aaaacauaac acuuacuaga     3060

cacuucgaug caaauguuga cgauagauac agaagaaugc uaaaagcaca aaaggccgaa     3120

aaagcaggug aagacuuuga uagugaagau uuaugcgaug accuaguauu uucuucauuc     3180

ggcgcaucaa guuuauauuc aaauaaagca cgaaaaguaa cgauaucucc caugggcgug     3240

cuacgagacc caggguucaa aggcuga                                         3267


<210>  5055
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5055
augaagaaau ugguauuggg guuagacaua ggugucgcaa gcgucgguug gggaaucaua       60

gacgcagaaa auggagacgu acuagacgcc ggcguaagau uauucagcuc aagagacgcc      120

agugaaaaug agucacgcag aaccucaaga agacaaucaa gaagacucuc aagaagaaag      180

aaacauagga gagaacgcau uaaacaacua cuauuagcac acgacaugga cguaugugau      240

auaguaaaug aaaauccaua ccaacuuaga guaaaaggac uaaacgaaaa acuagaaaua      300

ucggaaauuu acgucgcacu aacacaccuu gcuaaaaggc ggggaguguc auaucuagac      360

gacgucgucg aagaaggggg agccauaaaa agugaacacu uaaacaaaaa uauggaagaa      420

auaaaaucag gaaaaaugcc cugcgaaaua caauuagaaa gauucgaaaa auacggucaa      480

guaagaggua ugauagaaau caaccaagac acagaagaau cuucauuccu aguaaacauu      540

uucagaacau ccgacuauau aaaagaagcg aaaacaaucu uagcaacaca acaaaaauuu      600

cacaaacaaa uuacaaauga guucauagac gaauauauaa auauaauggc auccaagaga      660

gacuacuuua uaggaccagg aaacgaaaaa ucaagaaccg acuacggcgu uuacagaaca      720

aacggcgaua caugggacaa uuuauucgaa auuuuaauag gaaaauguag cguguuucca      780

gaagaaacaa gagcagcaag aagcaguuac guagcacaag aauuuaaccu ucugaacgau      840

cuuaacaauu uaaaaauuuc aucacuagaa ucaggaaaau uaucaacaag ucagaaacac      900

gacaucgugg aaaaggucaa aaauagcaag aaauuuggca ccaaagauuu gcuaaaauac      960

auagcaagug uaugugaaug uaaaauagaa aauauagacg gaugccgaaa agacaaaaau     1020

agaaaaccca ucaugcauac auuugaaaac uauaggaaag uacauaaugc cuuaaacgaa     1080

gcggguguag accuacgacu uucugaagca gauuacgacg aguuaaugaa aaucaugacg     1140

cucaauucug aaacacuaga gauaaagaaa caauguaaag aaaaacuacc acauuuaaca     1200

gaagauuacc uaaauguaug ucuugucuua aacaagaaag gagcauucuc aggauggcac     1260

gcucuauccc uaaaagcuau gaaauuaaug augccagaau uauacggaac aagcaaaaau     1320

cagaugcaga uaauccauac uaugcaacuc ggcaaagcau auaucaaacc uuuaaaccaa     1380

caaaaauauc uaaauaguga agcaauuuug gaggagauau auaacccuau cgcagcaaga     1440

agcaucagac aaaccauaaa agugacuaac gcgauacuaa agaaauacgg accuuuagac     1500

agcguaauca uagaaaugcc acgugacuac caaaccucag aagacgaaag aaaaaugaua     1560

gagaaaaugc aaaaagaaaa cgaaaaggaa aagaaagccg cacuaaaaag agcaagagaa     1620

gaauacccau ucccagacga ggcuuuuaga aaucacaaag aacuagccac gaaacuacga     1680

cuaugguacc aacaagauca auucugugua uacucuggaa ggaaaauuuc aguaguagau     1740

cuaguuagaa auccaaauca auucgaaaua gaccauauaa ucccacaauc aguaucccua     1800

gacgacaguc uaaauaauaa aguucuaugu uucagugaag aaaaccaaaa gaaaggacaa     1860

caaaccccuu ucuacuacca caaaggaaaa ucaggaaacu ggacuuacga acaauauaaa     1920

uuauucgucg aaaaacuauu ugacaaaaag aaaauaucga auaagaaacg agaacuacua     1980

uuguuugaag aagauauaaa caaaugggau gucagaagag gcuucauagc acguaaucua     2040

guugauacua gauaugcaag cagaguaauc cuaaacagcu accagcaaua uuucggaaac     2100

cacgaaaugg gcacaaaagu aaaaguggua agaggaaaau ucaccuccca auuacgaaag     2160

aaauggaaaa uauucaaaga cagaaacgag agucacuccc accacgcuau agaugcauua     2220

auugucgcag caacagcaaa ucuaagacuu uggaagaaaa auaaacuagg aaucuccgac     2280

gaaggaaaag uauacgauaa agaaacuggc gaaauacugc cauuugagaa auuaacagcc     2340

uccgaguaca augaaaugau gaacaaagaa ccauaucuca auuucaaaac aaaacuacaa     2400

aauauagaga caaaaguaaa auauagcuau aaaguaucga agaaaguuaa uagaaaaaua     2460

agcaacgaua cuauauauuc aacaagagaa cgagacggca uagacuauau uguagguaaa     2520

auuaaaaaca uauacgacgu aaaaaccuac aaagaccaau uccuaaaacg auauaagaaa     2580

aacaaagaac aauuccuaau guaccaacac gacccacaaa cauuugcaau auuagaaagu     2640

auaauggcca cuuacccaga cgcggagaau ccauuuuuag ccuacaagaa agaacacgga     2700

guaauaagaa aauaugccaa aaacaaagcg ggagcaccag uaacauccuu aaaauuuaua     2760

gacggcgcau uaggacagaa aauagauguc acuuauaaaa acaaagcagc auuacccaaa     2820

aacaagaaag uaguacuaca aucaauaaaa ccauaucgcg ccgacguaua cuacgacgaa     2880

ggaaacaaaa gauaucaccu aauaggagua aaauauucuc uauucucauu uucaaaacaa     2940

ggauauguca uaaaccccau agcuuaucaa aaacuucuag aaaaagaaca aauucauagc     3000

gaaaugaaau uuuguuucag ucuauauaga gaagacauaa uagaaucagg agaagaccca     3060

gacucacuga caagauaucg guuuuggagc aaaaaugaau cagguaaaaa uaaaauagaa     3120

guaaaaccua uugaaaaacc aaacuuugaa gacagaaaag uaaucacaau aacaaagaaa     3180

aucaaaauac ugaacaaaua ucaaacagau guauuaggaa auucuuucaa agcacaagaa     3240

gaaaaagauc caagaucaau cgaauga                                         3267


<210>  5056
<211>  3267
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5056
augaacuaua agaugggauu agauauagga auugcaagcg ucgguugggc aguaauaaau       60

cuagaucuaa aaagaauaga agacuuaggc guacgaauau uugacaaagc cgaacacccu      120

caaaauggcg aaagccucgc ucuuccaaga agaauagcaa gaucagcaag acgcagacua      180

agaagaagaa aacaucgacu agaacgcaua cgaagauuac ucguaucuga aaacguucuu      240

acaaaagagg aaaugaacuu auuauuuaaa caaaaaaaac aaauagacgu guggcagcug      300

agaguugacg cacuagaacg uaaacucaac aacgacgaau uagcaagggu auuacuacau      360

uuagccaaga gaagaggcuu caaaaguaau agaaaauccg aaaggaauuc caaagaaagc      420

agcgaauucc uaaaaaauau cgaggaaaac caauccauuu uagcccaaua cagaagugua      480

ggcgaaauga uagugaaaga cucaaaauuc gccuaccaca aacgaaauaa acuagacuca      540

uacagcaaua ugauagcaag agaugaucua gaaagggaaa uaaaacuaau auucgaaaaa      600

caacgagaau ucaauaaucc cguaugcacc gaacgauuag aagaaaaaua uuuaaauauc      660

uggucuucac aacgaccauu ugcgaguaaa gaggauaucg aaaaaaaagu aggauucugc      720

acuuucgaac cuaaagaaaa aagggcaccg aaagccacuu auacauucca guccuucauu      780

guaugggagc auauaaacaa auuacgauug guaucaccag augaaaccag agcauuaaca      840

gaaauagaaa gaaaccuccu auacaaacaa gcuuuuagua aaaauaagau gacauauuac      900

gacauuagga aacuacuaaa ccuauccgac gauauacauu uuaaaggucu ucuuuaugac      960

cccaaaaguu cucucaaaca aauugagaac auaagauuuc uagaauuaga cagcuaccac     1020

aagauccgaa aaugcauaga gaacgucuac ggaaaagaug gaauaagaau guucaacgaa     1080

acagauauug auacuuucgg uuacgcauua acaaucuuua aggaugauga agauaucguc     1140

gcauaccuuc aaaaugaaua uauaacaaaa aauggaaaaa gagugagcaa ccucgcaaac     1200

aaaguuuaug acaaaagccu aauagaugaa uuacucaacc uauccuucag caaauuugcu     1260

cauuuaagua ugaaagccau aagaaacaua cuaccauaca uggagcaagg cgaaauauac     1320

uccaaggcgu gugaacuagc agguuauaac uucacagguc ccaaaaaaaa agagaaagca     1380

uuacuccuac cuguuauucc caacauagcc aauccuguug uuaugagagc ucuaacacaa     1440

ucaagaaaag uagucaacgc aaucauaaaa aaauacggaa guccggucuc aauacacaua     1500

gagcuggccc gugaucuauc acacagcuuc gacgaaagaa aaaaaauuca aaaagaucaa     1560

acagaaaacc gaaaaaaaaa cgaaacagcc auaaaacaac uaauagaaua cgaacuaacc     1620

aaaaacccua ccggacuaga uauaguaaaa uuuaaacuau ggagugaaca acaaggcagg     1680

uguauguauu ccuuaaaacc caucgaauua gaacgacuau uagaaccagg cuacguagag     1740

guagaccaca uccuacccua cuccagaagu cuagaugacu cuuaugcgaa caaaguacuu     1800

guacuaacaa aagaaaaccg agaaaaagga aaccacacac cgguugaaua uuuaggacua     1860

gguagcgaaa gauggaaaaa auucgaaaaa uuugugcuag ccaacaaaca auuuucaaaa     1920

aaaaaaaaac aaaauuuacu aagauuacga uacgaagaaa cagaagaaaa agaguuuaag     1980

gaacgaaacc uaaacgauac cagauacaua ucaaaauucu uugcuaauuu uauaaaagaa     2040

cauuuaaagu ucgcagacgg agacggagga caaaaaguuu acacaauaaa cggaaaaaua     2100

accgcccauc uaagaucuag augggacuuc aacaaaaaua gagaggaauc agaccuacau     2160

caugccguag augcagucau agucgcaugc gcaacccaag gaaugauaaa aaaaauaaca     2220

gaauuuuaca aagcaagaga acaaaauaaa gagagcgcaa aaaaaaaaga acccauauuc     2280

ccccaaccau ggccgcacuu cgccgacgaa cuaaaagcca gguuauccaa auucccgcag     2340

gaaaguauag aagcuuucgc acucggaaac uacgauagga aaaagcucga aucgcuaaga     2400

ccuguauucg uaucuagaau gcccaaaaga agugucacag gugccgcuca ccaagaaacc     2460

uuacgcagau guguaggaau ugacgaacaa ucgggaaaaa uacaaaccgc aguaaaaacc     2520

aaacucuccg auauaaaacu cgauaaagac ggacauuucc cuauguauca aaaagaauca     2580

gacccgcgaa cauacgaagc uaucagacaa cgauuacucg aacauaauaa cgaccccaaa     2640

aaagcauuuc aagagccuuu auauaaacca aaaaaaaaug gagaaccagg cccagucauc     2700

cgaacaguga aaaucauaga cacaaaaaau aaaguaguac acuuagaugg cagcaaaaca     2760

guagcuuaca acucaaauau ugucagaaca gaugucuucg aaaaagacgg gaaauauuac     2820

uguguuccug uuuacaccau ggacauaaug aaagguaccc uaccaaacaa ggcaauagag     2880

gccaacaaac cauacucuga auggaaagaa augacugaag aauauacauu ucaauuuuca     2940

cuauuuccaa acgauuuagu aagaaucgua cuaccucgag agaaaacuau aaaaacauca     3000

acaaacgaag aaauaauaau aaaggacaua uucgcuuacu auaaaacuau agauucagca     3060

acaggugggc uagaacucau aagccaugau agaaauuuuu cacucagagg agucggaucu     3120

aaaacauuga aaagauucga aaaauaccaa gucgacgugu uaggaaacau ccauaaaguu     3180

aaaggagaaa agagaguugg auuagcagcu ccaacaaacc aaaaaaaagg aaaaacuguu     3240

gacucauuac aaucaguauc agacuga                                         3267


<210>  5057
<211>  3270
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5057
augacugccu uuaaaccaaa ccccuuaaau uauauacuag gauuggacau cggaauagca       60

agcguaggcu gggcaauggu agaaaucgau gauaaagaaa auccaguaag auuaauagac      120

cuaggagucc guguguucga aagagccgaa augcccaaaa cuggagauag uuuagccgca      180

gcgagaagau uagcacgcuc agucagacgu cuaacacgua ggagagcaca uagacuacua      240

cgagcaagac gauuacuaaa acacgaaggu gucuuaaaac cagaagauuu cgaugaaaau      300

ggccuaguca aaccauuacc auauauccca aaacaaccac guaauacacc cuggcaauug      360

agggcugccg ccuuagacag aaaacucaca ccguuagaau gggcugcagu acuacuacau      420

cuaguaaaac auagaggaua ccuaucacaa aggaaaaacg aaggggagac ugaugauaaa      480

gaacugggug cccuacuaaa aggaguagca gacaauaccc augcguuaca agcuggaaac      540

uuuagaacac cagcggaauu agcacucaau aaauucgaaa aagaaagugg ucacauaaga      600

aaucaaagag gugauuauuc ccacacauuu ucaaggaaag acuuacaagc agagcuagua      660

cuauuauucg aaaaacagaa agaauuugga aauccuuacg uaucagauga uuugaaaaac      720

ggaauagaaa aucuccuaau gacccagagg ccagcauuau caggagaugc uauucagaaa      780

augcuagguc acugcaccuu ugaaccggcc gaaccaaaag cugcuaaaaa uacuuauacc      840

gcagaaagau ucaucugguu aacaaaauug aacaacuuac guauuuuaaa ccaaggaaau      900

gaaagacccu uaacagaugc cgaaagaaca acacuuaugg augaacccua uaagaaauca      960

aaauuaacgu augcucaagc aagaaaauua cuagauuuag augauacagc guuuuucaaa     1020

ggauuacgau augguaaaga uaacgccgag ucugcaacuu uaauggaaau gaaagcauac     1080

caugccauuu cuagagcacu agaaaaagaa gguuugaaag auaagaaauc cccgcuaucg     1140

uuaaguccag aauuacaaga ugaaauugga acagcuuucu cauuauuuaa aaccgacgaa     1200

gaaauuacag gcagauuaaa gggaugucua cagcccgaaa uauuagacgc acuucugaag     1260

cauauuaguu ucgauaaauu cgugcaaaua agucucaaag cucuaagaag gauaguuccu     1320

cuuauggaac agggaaaacg auacgacgaa gcaugcgcgg aaaucuacgg ugaccaccau     1380

ggaagaagaa auagugaaga aacacuuuau cuaccaccga uaccagcaga cgaaauccga     1440

aauccaguug uucuuagagc auuaagucaa gcaagaaaag uaaucaacgg cguaggaaga     1500

agauauggua guccagcaag gauucauaua gaaacagcga gagaaguagg gaagaguuuc     1560

aaggacagaa aagaaauaga aaagagacaa gaagaaaaua gaaaagacag agaaaaagca     1620

gcagcagagu uccaagaaua cuucccaaac uuuguaggag aaccuaaaag uaaagacaua     1680

cuaaaacuua gacuuuacag acaacaaaau uguaaauguc ucuacucagg caaagaaaua     1740

aacuuaggca gacuaaacga aaaagguuac guugaaauag aucaugcucu ccccuucuca     1800

cgaacauggg acgacucguu caauaauaaa gugcuuguau uagguagcga aaaucaaaac     1860

aaaggaaaua gaacaccgua ugaauaccua aauggaaaag acaauucaag agaauggcaa     1920

gaguucaagg caagaguuga aacaagucgu uucccucaua guaagaaaca aagaauacua     1980

uuacaaaaau ucgacgaaga agguuuuaaa gagagaaacu uaaaugauac uagauaugua     2040

aauagauuuu uaugcaaauu uguagcggac cauaugcaau uaacuggcaa agguaaacgu     2100

agaguauuug cuucaaacgg acagaucacu aauuuacuaa gaggauuuug ggggcuaaga     2160

aagguaagag cugaaaauga caggcaucac gcuuuggacg cugucguagu ugcaugcagu     2220

acaguagcaa ugcagcagag aaucacacgu uuuguuagau auaaagaaau gaacgcauuu     2280

gacgguaaaa caguugauaa agaaacagga gaaguccuac aucagaaaac ccauuuuccc     2340

caaccuuggg aauuuuucgc acaagaaguc augauaagag uauuuggaaa accagacggg     2400

aaacccgagc uagaagaggc ugauacgcca gaaaagcuaa gagcauuauu agcagaaaag     2460

uuaucaucac guccugaagc aguacaugaa uacgucgcuc cacuauucgu aagcagggcc     2520

ccaaacagaa aaaugagugg ccaaggacau auggaaacaa uaaaaucccc gaaacgacuu     2580

gacgaaggaa uaagcguagu cagaguacca cuuacucaac uaaaacucaa agacuuagag     2640

aaaaugguua acagagaaag ggaaccaaag uuguaugaag cauuaaaagc acguuuggaa     2700

gcacacaaag augacccagc uaaggcguuu gcagaaccau ucuacaaaca agacaaagcu     2760

ggaaacagaa cccaacaagu uaaagccgua agaauagaac aagugcaaaa gacaggggua     2820

uggguaagaa accaaaaugg gauagccgau aaugcaacaa ugguccgcgu agauguauuu     2880

gaaaagggag gcaaauacua ccugguacca auauauucau ggcaaguagc aaaaggaauu     2940

uugccagaua aagcaauuau agcauuuaaa gacgaagaag aauggcaauu aauagacgau     3000

ucauuuaauu uuaaauuccc ccuucauccu aaugaucuug uagaaguaau aacuaagaaa     3060

gcgagaauau uuggauauuu ugccaguugu cauaggggua cgggaaauau aaacauaaga     3120

auacacgacu uagauaacaa aguaggcaaa aauggaaucc uggaaggaau aggaguaaaa     3180

acagcguuau cauuccaaaa auaucaaaua gaugaacucg gaaaagaaau ccgaccaugu     3240

agguuaaaga aaagaccucc uguacgauga                                      3270


<210>  5058
<211>  3270
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5058
augaacguaa gauauaaaau uggauuagac auagguauaa caucaguagg augggcagua       60

guaaaccuag acauaccucg aauagaagau cuaggaguca gaauauuuga ccgcgcagaa      120

aacccacaaa caggugaauc ccuagcuuua ccaaggagac uagcacguag ugcacgcaga      180

agauuaagaa gaaggaaaca cagacuagag cgcauaaggc gccucuuaau aagagaaggc      240

auauugauga aagaagaguu ggacaagcuc uuugaagaaa ggcaugaaau cgauguuugg      300

caauuaagag ucgaggcuuu agaucgaaaa uuaaacucug augaauuagc acguguuuua      360

cuccaucuag cuaaacgcag aggauuuaaa ucuaauagaa aaucagaaca uaacagucga      420

gaaaauagca caaugcuaaa acacauugaa gaaaauagag ccauccuauc cucauauaga      480

acaguugcgg aaaugauagu aaaagacccu aaauucgcau uacacaaacg uaacaaggga      540

gaacacuaua cuaauacaau cgccagagac gauuuagaac gagaaauaca auuaauuuuu      600

acaaaacaaa gagaguucgg uaauaugaac ugcacaaaag aauuugaaaa ugaauauauc      660

gcuauauggg caucucaaag acccuucgcu ucaaaggacg auauagaaaa gaaaguagga      720

uucugcacuu uugaaccaaa agagaaaaga gcccccaaag caaccuauac auuucagucc      780

uucauagcau gggaacacau aaacaaauua cggcuaguau cccccgcagg gacaagagga      840

uugacagacg aagaaagaag acuauuauau gaacaagcau uucagaaaaa uaaaauaaca      900

uaucacgaca uuaggacauu auugcgacua ccagaugaua cauauuucaa aggggucgug      960

uacgacagga acaugccacu gaaacaaaau gaaacaauaa gauuucuaga acuagaugcc     1020

uaccaccaaa uaagaaaagu uauagauaaa gcauacggaa aagguaaauc gucuuccuuu     1080

uuaccuauag auuucgauac auucggcuac gcacucacac ucuuuaaaga cgacgccgac     1140

guccgcaguu accuacaaaa cgaauacgaa caaaauggaa aaccaaugcc aaaccucgcc     1200

aauaaaaccu acgauaacga acucauugaa gaacucuuaa accuaaguuu uacuaaauuc     1260

ggacaucuaa gucugaaagc gcugcauaau auccuaccgu acauggagca aggagaagua     1320

uacagcucag caugcgaaag ggcuggauau aaauuuacag gaccaagaaa gaaacagaaa     1380

acaauguuac uaccaaauau cccuaguaua gcgaauccag uggucaugag agcacuaaca     1440

caagccagga aagucgugaa ugccauaauc aaaaaguaug gaucaccugu aagcauacau     1500

auagagcucg cuagagaccu aucacaaaca uucgacgaac gaagaaaaac uaaaagggaa     1560

caagacgaaa aucggaaaaa gaaugaaacu gccauaagac aacuaaugga guauggauua     1620

acacuaaauc caaccggaca cgauauugua aaguuuaaau uauggaguga acaaaacggc     1680

agaugcgcau acagucuaca accaauagaa aucgaaagac ucuuagaacc aggguauaca     1740

gaaguugacc acguaauucc auacaguaga ucauuggaug acagcuauac aaauaagguc     1800

cuaguauuaa ccaaagaaaa ccgugaaaaa ggaaauagaa caccagcaga guacuuagga     1860

guaggcacag aaagauggca ccaauucgaa acauuuguau uaaccaacaa acaauucucc     1920

aaaaagaaaa gagauagauu auuacgacug cauuacgaug aaaacgaaga aacugaguuc     1980

aaaaacagaa aucuaaacga caccagauac auuaguagau uuuucgccaa uuucauaaga     2040

gaacauuuaa aauuugcaga aucagaugac aaacaaaaag uuuacaccgu aaacggacga     2100

auaacugcac auuuacgaag cagaugggag uucaacaaaa auagagaaga auccgaccuc     2160

caucaugcag uagacgccgc aauaguugcg ugcacaacac caagcgauau agcaaaaauu     2220

acugccuucu aucaacgaag ggaacaaaau aaagaacuag caaagaaaac agaaccacac     2280

uuuccccaac cuuggccaca uuuugcagau gaacuacgag cgagacuauc caaacaucca     2340

aaagaaagua uuaaagcacu aaacuuagga aacuaugaca augaaaaacu agaaucauua     2400

cacccugucu uuguuuccag aaugccaaaa agaaguguaa caggagcugc ccaccaggaa     2460

acacuaagga gauauguagg cauagaugaa agauccggaa aaauucagac aguugucaaa     2520

acaaaacuau ccgaaauaaa acuagacgcc aguggccacu uccccaugua cggcaaagaa     2580

agugauccgc gaacauacga agcaauaaga caacgauuac uagaacacaa caacgaucca     2640

aagaaagcau uccaggaacc acuuuauaaa ccaaagaaaa auggagaacc uggcccuaua     2700

auccgaacug uaaaaaucau agacacuaaa aaucaaguag uaccguugaa ugacggcaaa     2760

acaguagcau auaacagcaa caucguaaga gucgauguau ucgaaaaaga cgguaaauac     2820

uauugcgucc ccguauauac aauggauaua augaaaggug uauugccaaa caaagcaaua     2880

gaaccaaaca aaccauauuc agaauggaaa gaaaugaccg aagauuauac uuuccaauuu     2940

agcuuauacc ccaacgaucu aauacgaaua gaauuaccua gggagaaagc uauaagaaca     3000

aguacgggag aagaaauaaa aaucaaagac uucuucgcuu auuacaaaac cauccacagc     3060

ggaacaggag gaauggaacu aauuucacac gaccgaucuu uuucucucag cggaguaggc     3120

ucaagaacuu uaaaaagguu ugaaaaauac caaguagaug uacuuggaaa uauauauaaa     3180

guccguggug aaaaacgugu aggacuugcc aguagcgcac auagcaaaac agguaaaacu     3240

guaaggccgc uucaaagcac cagagauuga                                      3270


<210>  5059
<211>  3273
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5059
augagaugga gauuagggcu agacaugggu gcagguuccc uagguuggug ugcaguuggu       60

cuagacgacc aagguuaccc ugacucaauu uuagcagcag gaagccauau uuuuggagac      120

gguagagcac ccucaagggg ugaucagcaa gggacaccac uaggcguaga aagacgagca      180

gcaaaagcga ugagacgacg acgagacaga uucuuaggga gacaagcaaa gcuguuagcu      240

agauuaacac ucgcagguuu auuuccagcc gaucccguaa gggcaaaagc acuagaauua      300

cuugaucccu auguucuaag agccagagca uuagaugaag cauuaccccc gcaccagcuc      360

gggagagcau ucuuccaucu uaaucaaaga agaggauuua aaucaaauag aaaaacggac      420

agaggaguug acgacgaagc aggaaaaauu gccauaggaa uagauagacu aagagcagcc      480

auagcggcag cagaagcuag aacuaugggg gaguucuugc auaaacgacg acaaucagca      540

ccggauguua aucagauacc uaguguuaga accaggcuua gaccagaaac aggagaggag      600

gcuaaaggag acggcuacga uuucuaucca ggaagagaau uaguagaaga agaguucgac      660

gcaauauggg acgcccaagc gccacaccac ccaagucugu ugacaccgga caauagggcg      720

uuacuucaug aagcaauauu cuaucaaaga ccacuuaaag cuccaaaaau aggaaaaugc      780

acauuacuaa acaacgaacc aagacuacca aaggcucacc cacucuuuca aagaagaaga      840

cuuuuggaag aaguuaacgc acucagaauu guaaggcccg gugaaguggc uacaaagcua      900

accugcgaac agagagacuu auugauguug aaauuaaaag acaaagcuaa agucagcuuc      960

gaaacacuaa gaucaaaagu acuaaaauua gaaccagaag caagauucaa uaaagaaggu     1020

gaaaacagaa cagaacuugu aggugaugaa guaugugcac aaauguccgc gaaaacaaga     1080

uucggaaaag caugggcuca uuuuaccgau gaaaaacgau gggcaauuuu aucaagacua     1140

auugaagaag aagacgagga aagauuagau acuugguuaa gaacagaaca uggucuugac     1200

gcugaacaag caagagcagu ugcgggugcc aggcuuccgg cuggguuugg cagguuuggu     1260

gaaacagcaa ccagaagacu aauagcagcu cuacaagcag aaguuaucgu guauagugaa     1320

gcaguuguag cagcaggacu aggacaucac agcgacaugc gcacugguga aguuuuagca     1380

gaacuucccu auuaugguau gacacuagaa aggcauuuau uaccgggaac aggagauaaa     1440

gccgauccag aagaacuccg aauuggacga cucacaaauc caaccguaca cauagcauua     1500

aauaggcuua gacguaucgu uaacagguua auaaaaacuu acggacgacc agaacaaauc     1560

gcaauagaac ugggcagaga acuaaagcua acagaugagg acaagcagag auuaagucga     1620

caaaacaaca gaaacagagc agaugcagaa gcaagaagua aaaaguuggc ggaauuagga     1680

caagcagaca auggugcaaa uagggcaaga cucaaguuau gggaagaguu aaacagagaa     1740

aaugcuuugg auagacgaug uauuuauagu ggucagcaaa uaucuaucgc aauguuauuc     1800

ucagaugcag uugaaguuga ucacauacug ccauuugaug caacguuaga ugauagcaac     1860

aauaaucgua uucuuuguuu aagagaaugg aaucgaagaa aaagaaaaca aaguccuuau     1920

gaagcauggg gccacaccga uuauuggcca gauauagcag cacgagcugc cagacuacca     1980

aaagaaaaga gauggcgcuu ugaaccagac gcuaugcaaa gauucagcgc agaagauaga     2040

uucauagcaa gacauuuagu agacacacaa uauaugagcc guuuagccag agaauaucuu     2100

gaagcauuau acccugaaaa aggagaaggc uccggucacg uauggguuuc accaggaaga     2160

cugacagaau uaauucguag aaaguuagga cuaaacgaau uauuaccaga ucacaauuuu     2220

gcugguguca aaaauagauu agaccacagg caccauacaa uagaugcauu cguaguagcc     2280

guaacagaua gaucacuucu ucagggaauu ucucgagcga gugccuugga aggagaggaa     2340

ggaagagaga gaguaauagu accugagcca ugggacgguu uucgagaagc ccucagaaca     2400

acucuaaaua caaucacagu auggcacaga caagaccacg ggacagcauc uaaagcagga     2460

uuagcucaag ggagagacca aacagcagga aaauuacaua augauacagc auauggacuu     2520

acugggagac aagacaccaa ggguaaugau uuaguaguga gcagaaagcc auuaaugagc     2580

uuaaagccag cagauauaga accagucccu gaugucgcac cuauuacuaa aggaguuaga     2640

gauagagauc uaagaaaagc gcuagcucac uuuacacagg caacugaagg caaaggauuc     2700

cuacaagcgc uagaaagauu cacacgacuu gguccgcuuc auuucagagg uauucgacga     2760

gugcgauuga uccaaccauu agcuguaaua ccuauaaaag guccaggugg ggucccuuau     2820

aaaggauacc uaggagggga aaauuuucga ugugacguau gggaaaugcc agacggcgca     2880

uggaaaccug aagugacauc gauguuucaa gcacaucagc caggcuggac aucagcaauu     2940

agagcagcau guccuacagc caggaaagug cuuucacugc acaaaagaga uaugauagca     3000

auagaacgag aagaaggugu gagaacguug augguagucg cgcaaauuug guccaauggc     3060

caaguaacac ucguugaacc ccacgaggcc ggcgacuuaa acaagaggaa uaaaucugaa     3120

gaugauccau uuggauauuu uaugccaaca gcuggaggcc uaaaaagauu aaaagcaaga     3180

caagucagag uagaugaacu cggacguguc caagauccag guuuuccagc aaggaaagcc     3240

cguagaagga ccagaccaaa acagguggau uga                                  3273


<210>  5060
<211>  3273
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5060
auggauugga gacuuggccu agauuuaggu gcaaauucgc ugggaugggc agcauugcgg       60

uuagacaggg caggagaucc aauuggcaua cuagcuaugg guucucguau cuucucagau      120

ggacgagacc caaaauccgg agcuucuuua gcuguagaua ggcgagcagc cagagcaaug      180

agaagacgaa gggacagguu caaacagagg caggccgcac uacuaaaaua cuuaaucaga      240

gacggccucu uuccagcaga ccaagccgac agacaaagau uagcagauga agauccauau      300

gcauuaagag ccagagcccu agaugaagaa cuaucuuuau ucgaaauagg uagagcucua      360

uuucaccuga aucaaagaag aggauuccag agcaacagaa aggcagacag aggccaagau      420

gaugaagccg gaaaaauagc cgugggagua gacagacuaa accaacagau ggaagaacag      480

ggcgcaagaa cauuggggga auuuuuacau caaagaagaa gggcagcaac agaugccaac      540

cauauaccau cuguacguac uagauuaaga ccagaaacag gggaaggggc aaagggagac      600

ggauaugauu uuuacccauc gagagcacua auagccgagg aauuugaaga aauaugggcu      660

gcacaagcac cacacuaucc agauacacua acgcaacaag uacaugacag auuauaugaa      720

auuauauucc aucagagacc ccuaagagca ccacaagucg gacaguguac gcuuguugcc      780

ggagaaacaa ggcuaccaaa ggcucaccca cuauuccaga aaagacgauu auuagaggaa      840

aucaaugcuc uaaugauagu aaggcccggu gagauagcuc aacgauuaac uccugaagaa      900

agggauacgu uguaccucaa acuuaaagau aaacgaacag uuaguuuugc gacauugcga      960

aagaccuuga aacuagacuc ugcagcuaga uuuaauaaag aaagugaaaa cagaacugaa     1020

uuaaaaggag augaaguagc ugcagaacua ggagcaaaaa caaggcuagg aacuagaugg     1080

caacaccuga gcggggauca acaaugggaa guuguacaca gacuaauggc acuagaaagu     1140

caagcggaug aagcagcauu uagagauugg auuucccaaa cauacgaccu auuaccagaa     1200

caagcagcug caguauuagg ggcaagauua ccacaaggac augguagauu uggaccaaca     1260

gcgacaacaa cccugauaga cucauuauua cacgacagaa cugaagaugg gcggguggua     1320

guguauagcg aggcugucca acgaagcgga uuaggucauc auagcgaucu acgucacgau     1380

agagaugacg aagauguaac cgaauuacca uacuauggag uagcuuuaga aagacauaua     1440

augccgggaa cugcagaacc aacagauccu gaagaaauga gaauuggucg acuaaccaac     1500

ccaacuguac auaucggcuu gaaucaacug agacaugugg uaaauagauu aauuagagcc     1560

uauggaccac caaaagaaau agcaauagaa uuagcaagag agcuaaaacu cagcgaggac     1620

gaaaagaaga aaagaaauag cgaaaauaga gauaauagac uggcugcgga aggaagaagc     1680

aagaaauuag aagaaauaag acaaagagau acgggcgcaa aucgagcauu auuaaaauua     1740

ugggaagagu uaaauccugg gaauguuuua gaccgaaggu guauauauac aggcgaacaa     1800

auaagcaucg auaugcuauu uucagauaga guagaaguug aucauauccu gccauucgcc     1860

cagacacucg acgauucaaa uggaaauaaa cuucuauguu uaagggaagc gaacagagag     1920

aaaagaagaa ggucaccgua ugaggcuugg ggacauacag auagauggcc agacauugcu     1980

gaaagagcuu cucgauuacc caaaccuaaa agauggagau uugaaccaga cgcuaugcaa     2040

cgauuugcaa cugaagacgg cuuucuagca cgccaucuag uagacacaca auauuuagga     2100

agauuggcaa gagaauaucu aucaugguug uauccaggaa gaggagaagg uagugggcau     2160

guguggguaa gcccuggaag gcuuacugaa augguaagaa gaaaacuugg acuuaaugac     2220

uuacuaccag accacaauuu uggaggagga gcugaccagc cuaaaaaccg auuagaccau     2280

agacaucaug caaucgaugc gcuaguaguc gcaauaguag aucgagggau gcuacaagcc     2340

auagcaagag caucaggcca agauggguca gaaggaagag aaaggauuca caucccagcc     2400

ccaugggaug guuuuaggga ugaucuuaga guugccguca auggaauaau aguuagccac     2460

agaccagauc acgguacugu agcuaaagcg ggucuagcac gagguaggga ucaaacagcu     2520

ggcaggcuac auaaugauac agcguauggu uuaacaggag aaacagaugc aaaaggaaac     2580

agcauugugg ugcgacggau accauugagc gcuauaaaga aacccgcaga uauagccaga     2640

guaagagauc cacucuuaag aaaugcuuua gcugaauuua cuaaaggacu agauggaaaa     2700

gcauuugaag cucgaauugg aagauuccac gaacuaggcc cacuacuuua uagaggaauc     2760

agaaggguac gagucguaga aacacuaacc guaaucccua uacgggaugc agaagguagg     2820

gcauauaaag guuauaaggg cgacuccaac uauagguacg auguauggga aaugccagau     2880

ggaaaaugga gaugucaagu cguaucaaug uuugaugcac aucagagcac ugaacaagcu     2940

cgaccacauc cagcagcucg aaaaauacuu aguuuacaaa gaaacgacau acuugcaauu     3000

gaaagaacug aaggcgauag ggaauuagug cgcguaguua aguucucgga aaaacaauuu     3060

gcugucgcuc cuccacauga agguggaucc uuaaaaacac gagacggaaa cgcagaugau     3120

ccuuuuagau auuuauaccc gucaccaaau acacucaaag gauggagggc aaggcaaguu     3180

agaauagaug aacugggccg uguacuagac ccagguuucc cagcacgcaa gucuguaaga     3240

agaacuagac cuagggcagc uccuguagau uga                                  3273


<210>  5061
<211>  3276
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5061
auguacucua acuuacuauu uucauucgac auaggaacaa acucaauagg augguguguu       60

uucgcauuag augaagcagg agaaccuuau agaauagugg accucggagc aagaauauac      120

gcagauggaa gagacccaca auccaagaca agucuagcag uugcaagaag agaagccaga      180

gcaaugagca gaagaagaga cagguaucua agaagacgaa aggcaacauu acgcacaaug      240

auagaauacg gccuuaugcc aggagacaaa gccggccaag aagcaguauu acgcgaaacc      300

ggagauagag aaggaggaga ugaaaguuuu aauccauaug cucuaagagc aagagcacuu      360

agcgaaaaac uaccaccggc auauauagga agagugcuau uucaucuagg acaaagacga      420

ggcuuuaaau caaauagaaa aacugauaga aaagacaaug auaaagguaa aauagcauug      480

ggaauagacg aauuaaccgc agcaaugcau agaucaggaa guccaacacu aggagcuugg      540

cuagcaauga gacgugccga aggacauagc guacgauuaa gagcugguuc cgaaguauuu      600

gaugcagaag gauaugcuuu uuauccagag agaagccucc uagaagauga auuuagacaa      660

aucugggacg cucaagcagu ccaccauccc gacaucuuaa ccccagaaag acgagcgcau      720

cuuuucagag uaauguuuua ccaacgaccg uuaaagaaac cauuaguagg acgauguagu      780

uuuaacccag cugaguucag auugagucgu gcacaucccc uauuccaaga auuucggcua      840

uauaaagaga uaaaugaucu agaagucguc cuaccagacc aaucacacag aaaauuaaca      900

uuagaugaaa ggaacgcucu uguugcaaaa uugaaaacuu caagaaaagc aaguuuuagu      960

gcucuaagaa aaacguuaaa acuuacccca gacuuaguau ucaacaaaga aucagaagca     1020

agaaaagaau uauuggguga ugaaauuuac aguguacugg cagacgcaaa aauguucggu     1080

gcacguuggg gugguuuuag uagagcucgu caaugggaaa ucauaacaag auuaaaagaa     1140

gaagaagacc cugcaagucu aaguucaugg uuaaaauccg aguucggccu agaugacgaa     1200

agaguaacag caauagcaaa cgcgagcuua ccugaugguu auggacgauu gggagaaaca     1260

gcacucgcaa guaugcugga agaacuaaag acagcaguaa uaccggaauc agaagcagcg     1320

agacuaugug gauacgauca cagcaacuua gcuaaagaac aagaugaagg acucgauacc     1380

cuucccgagu accaggagau uuuagaacga cacauaccuc cagguacugg agaucccgau     1440

gauaucuacg auuuaagaaa aggacggauc accaacccca caguacauau aggacugaac     1500

caacuaagaa gaguaguaaa ugcucuaauu caucgcuuug gacguccgca aagaauagua     1560

guagaauugg caagagaccu acaacuaucc gaaaaacaaa aagaugacgu uaauagaagg     1620

auagcgaaaa acacaagaga agcagaagga cgaucucaaa aacuuauaga gaugggacaa     1680

uuagacacag gcuauaacag acuucuucua aaauuauggg aagaauuaaa uuuagacaaa     1740

ccagaagacc gaguguguau cuacagcgga aaaccaauug gaauagcaau gcuauuuagc     1800

ggagaaguug augucgacca uauauuaccg uggagcaaaa cacuagauga cucacaggcu     1860

aauaaacuuu uauguuuaaa aucugcaaau agacaaaagc gaaauagaac accaucggau     1920

gucccagaau ggagagauag auaugacgaa gugcuugcac gcgcagcuag acuaccaaga     1980

aauaagagau ggagauucgc ggccgaugca augcaacaau uugaagcgga aggaggauuu     2040

uuggcacgac aacuaacaga uacccaauau uuaaguagga uggcauuaga auauuugagc     2100

gcauuauauc ccagugaaga agcggauaaa aguggagaau uaagacaaag aaaaaggguc     2160

cauguaguac caggaaggcu aacugaacua cugagacgua auuggggacu uaacacauua     2220

cuacccgacc auaaucuagg ugaaauggca caagagaaaa auagaaggga ccacagacau     2280

cacgcuaucg augcagcagu uauaggaacu acgagucggu cuuuacugca aagaaugagu     2340

ggggcagcag cacgguugga cgauguagca uucgaugacu uaguucggac ugugguaaaa     2400

gauaaucccc cguggccggg auuuagggaa gaguuacuug cugcuauagg ucgcguuaca     2460

guaucccaua aacccgauca uggaacugua aguagagcag cauaugcaca agguaaagga     2520

cagacggccg guaaacuaca caacgauacu gcauauggac uaacaggauu ggucgaugcg     2580

aaaggaagcc cccugguggu caggagaaaa ccauucaugg cucuagaagu aaaagacauu     2640

gcuaguauac gcgauacgga acuccaaagc gcuuuauacg gugcaauagg uaguuuaaca     2700

gaaaagaaag cucuacaaga ggcacuaaua agauuuaggg auacacaucc gcaauuuaaa     2760

ggaauaagaa gaguuagagu ucucgaaaca uuaucuguaa uaccaauagu agacagggac     2820

ggcaaggcau auaaaggaua uaaaggagac gcaaacuacc gcuaugaggu augggaaacu     2880

cuagauggga aauggcauac ugaaguaguu ucuauguuug augcacauca accagguugg     2940

caaaguccau uucauaaaca gaauccugca gcaagacgug uccuaaaauu acaacaaaac     3000

gacauggucg cauaugaaca uccagcugau ggguacacaa uagcuagagu uguuaaauuc     3060

agcaacgaua aaagaauuua cuucgcaucu cauagagaaa guggaucauu aaaagcaaga     3120

gaugcagaua aaggagaucc auucucuuau uuugcuaaag caaacaaugg ucuaagagau     3180

auuaaaugua gacaagucag aauagaugcg gccggaagaa uuuuugaucc aggaccucaa     3240

gaucgggaga cgcgacaagc aaauaaaggu guuuga                               3276


<210>  5062
<211>  3279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5062
auggcauaca caaugggcau agauguggga auagcaucuu gugguugggc aauaguugau       60

cuagaaagac agagaauaau agauauaggu guaaggacgu ucgaaaaagc agaaaaccca      120

aaaaacggag aagcucuagc aguaccuaga agagaagcca gaucaucaag acggagauua      180

agacguaaaa aacauagaau cgaaagacua aaauacaugu uuguccggaa cgggcuagcu      240

guugauauac aacaccuaga acaaacucua cgcagccaga acgaaauuga cguauggcaa      300

uugagagugg auggacuuga cagaaugcua acacaaaagg aauggcuccg aguuuugaua      360

cauuuagcuc aaagaagagg auuucaauca aacagaaaaa cggacggaag cucugaggac      420

ggacaagugu ugguuaacgu caccgagaac gacagacuaa uggaggaaaa agauuauaga      480

acaguagccg aaaugauggu caaagaugaa aaauucagcg accauaaaag aaacaaaaac      540

ggcaacuacc acggugucgu uucuagagcc ucauuauuag uagaaauaca uacauuauuc      600

gaaucacaaa ggcaacauca caacuuuuua gcaagcaaag auuucgagcu ugaauauauu      660

aauauaugga gcucacaaag gccaguagca acaaaagacc aaauagaaaa aaugauaggc      720

acaugcacau uuuuaccaaa agagaaaaga gcuccaaaag caucauggca uuuucaauac      780

uuuaugcuac uacaaacuau uaaccacauc agaauaacua augcccaggg aaccagacca      840

uuaaauaaag aggaaauaga gcaaguuguu aauauggcac ucacaaaaag uaaaguaagu      900

uaccaugaua uaagaaaagu cuuagaucua ucagaagaau aucaauucgu gggacuggac      960

uacggaaaag aggacgaaaa aaaaaaagua gaaagcaaag aaacuauaau uaagcuugau     1020

gacuaccaca aauuaaacaa aauauuuaac gaaguagagc ucgcaaaagg cgaaacaugg     1080

gaagcaguug auuacgacac uguagcauac gcacuaacau ucuucaaaga cgaugaagac     1140

auuagggauu acuugcaaaa uaaauauaaa gacucuaaaa auagauuagu gaaaaaugua     1200

gcaaacaaag aauacacaaa ugaauuaaua gaaaaagucu caacacuuag cuucagaaaa     1260

guaggccacc uuucacuaaa agcuuuaaga aaaauaauac cauuccucga acaagggaug     1320

accuaugaua aagcguguca agccgcugga uuuaauuuuc aaggaauaag uaaaaaaaaa     1380

agaucagucg uacucccggu aauugaccaa auaaguaacc caguaguaaa cagagcacua     1440

acucagacua gaaaaguaau aaaugcauua auaaaaaaau acggaagccc ggagacaaua     1500

cacauagaga ccgcuagaga auuaucaaaa cgauucgaug aacgaaaaaa uauaacaaaa     1560

gauuacaaag aaaauaggga uaaaaacgaa agagcaaaaa agcacuuauc agaauuaggu     1620

auaaucaauc cgaccggcuu agauauagua aaauauaaau uaugguguga acaacaaggg     1680

agauguaugu acagcaacca accaauauca uucgaaaggu ugagagaaag cggcuauaca     1740

gaaguagauc auauaauacc auacucaaga agcaugaacg acuccuacaa uaauaggguu     1800

uuaguuauga cccgggaaaa cagagagaaa uccaaucaaa ccccuuacga auacauggga     1860

aaugacacuc aaagauggga ugaauucgag caaaggguga cgacaaaucc acaaauaaaa     1920

aaagaaaaaa gacaaaacuu gcuacuaaaa ggauuuacaa auagaagaga acucgaaaug     1980

uuggaacgaa acuuaaacga cacaagacau auaacgaaau acuuaaguca cuuuauaucc     2040

acuaacuuag aguucucccc cucugauaag aaaaaaaaag uaguaaacac aagcggaaga     2100

auaaccagcc aucuacgauc gaggugggga cuagaaaaaa auagggaaca aaaugacuua     2160

caccacgcua uggaugccgu uguuauagca guaacuucag acucuuuuau acaacaagua     2220

acgaauuacu auaaacgaaa agaaagaaga gaacuaaacg gcgaugauaa auuccccuua     2280

ccuuggaaau uuuuccgcga agaagucaua gcgagguuau cgccaaaccc uaaagaacag     2340

auagaagcac uucccaacca cuucuacagu gaagacgaac uagcagaucu acaacccauc     2400

uuuguuagca gaaugccgaa acguaguauu acaggcgagg cacaccaagc acaauuccga     2460

agaguaguag gaaaaacaaa agaaggaaaa aauaucacag caaaaaaaac ugcacugguu     2520

gacaucagcu acgacaaaaa cggagauuuu aauauguaug gaagagaaac agauccggca     2580

acauacgagg ccauuaagga gagauaccua gaguuuggag gaaaugcuaa aaaagcauuc     2640

agcacagauc uccacaaacc uaagaaggac ggaacaaagg guccguuaau uaaaucagug     2700

agaauaaugg aaaacaaaac acuaguccac ccaguuaaca aaggaaaagg cguaguuuac     2760

aacagcagua ucguaagaac ugauguauuu caaaggaaag aaaaauacua cuuauuaccu     2820

gucuauguua cugauguaac aaaaggaaaa cuucccaaca aaguuaucgc agcuaaaaaa     2880

ggauaugaug auuggauuga aguggacgau agcuuuacau ucuuguacuc guuauaucca     2940

aaugaccuaa ucuucauaag gcaaaaucca aaaaaaaaga uuucguuaaa aaagcgaaua     3000

gaaagccauu caauuagcga uucaaaggaa gugcaagaaa uauuaacaua uuacaaaggu     3060

guagacagcu caacggcugc caucgaguuu auaacccaug acggaucaua uuacgcaaag     3120

gggguaggug uacaaaauuu agacugcuuu gaaaaauauc aaguugacau auugggaaac     3180

uacuccaaag uuaaaggaga aaaaagauua gaauuagaaa caagugacaa uaaccauaaa     3240

ggaaaagacg uaaacagcau uaaaucuaca agucgcuga                            3279


<210>  5063
<211>  3279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5063
augaaauaca gcauaggccu ggauauuggc auagcaagug uaggcuggag uguaaucaac       60

aaagacaagg agagaauaga agacaugggu gucagaauau uucaaaaagc agaaaaucca      120

aaagauggau caucacuagc uucaucaaga agagaaaaac gaggaucacg ccguagaaac      180

agacgcaaaa aacaucgacu cgauagaaua aaaaacaucu uaugcgaaag cggacuuguc      240

aaaaaaaaug aaauagaaaa aauauacaaa aaugccuauc uuaaaucucc augggaauua      300

agagcuaaau ccuuagaagc caagauauca aacaaagaaa uugcacagau auuacuacac      360

aucgcaaaaa gaaggggauu caaaagcuuc agaaaaacag accgaaaugc ggaugauacg      420

ggaaaauugc uaucgggaau acaagaaaac aaaaaaauua uggaagaaaa aggauauuua      480

acgauuggcg acaugguugc caaggacccc aaauuuaaua cgcacguaag aaacaaagca      540

ggauccuauu uauuuucauu uuccaggaaa uuacuagaag acgaagucag aaaaauacaa      600

gcaaaacaaa aagaauuagg gaacacacac uucaccgaug acguucuaga aaaauacaua      660

gaaguauuca auucacaaag gaauuucgau gaaggaccaa gcaaaccuag cccauauuac      720

agcgaaauug gacaaauugc uaaaaugaua ggaaauugua cauucgaauc aucagaaaaa      780

aggacagcaa aaaacacaug gucgggcgaa cgcuucguau ucuugcagaa acuaaacaau      840

uuuagaauag uaggcuuauc aggaaaacga ccauuaacug aagaagaaag agauauagua      900

gagaaagaag ucuaccucaa aaaagaaguc agauaugaaa aacuaagaaa gauuuuauau      960

cugaaggaag aagaaagauu cggggaccua aacuacagca aagaugaaaa gcaagacaaa     1020

aaaacagaaa aaacuaaauu cauaucauua auagguaacu acacaaucaa aaaacuaaac     1080

uuaucagaaa aacucaaaag cgaaauugaa gaagauaaga gcaaacucga caaaauaauu     1140

gaaauauuaa cguuuaauaa auccgacaag acuauagaau caaaucuaaa aaaacuagaa     1200

cuaaguagag aagauauaga aauccuacua uccgaagagu ucagugguac acugaauuua     1260

ucuuuaaaag caauaaaaaa aauccucccu uauuuagaaa aaggacuauc auauaaugaa     1320

gcaugugaaa aagcugacua ugacuacaag aacaauggua ucaaguucaa aagaggagaa     1380

uuacuaccag uaguagacaa ggaucugaua gcaaaccccg ugguauuacg agcaauuuca     1440

caaacacgaa aaguagucaa cgcaaucaua agaaaauacg gaacaccaca cacaauacau     1500

guagaaguag cacgagacuu agcgaaaucg uaugacgaca gacaaaccau uaucaaagaa     1560

aacaaaaaaa gagaacuaga aaaugaaaaa acaaaaaaau ucauaucaga agaauucgga     1620

aucaaaaaug uaaaaggaaa auuauuacua aaauaccgac uuuaccaaga acaggaagga     1680

agaugcgcau acucacgaaa ggaacuauca cuauccgaag uaauacuaga cgaaaguaug     1740

accgacauag aucauauaau cccguacuca agaagcaugg augacucuua cagcaacaaa     1800

guauugguac ucaguggaga aaacagaaaa aaaucuaacu uguuaccaaa agaauacuuc     1860

gauagacaag gaagagauug ggauacauuu guauugaaug uaaaagcaau gaagauacau     1920

ccgaggaaaa agagcaaucu acugaaagaa aaauuuacaa gagaagacaa caaggauugg     1980

aaaaguaggg cacuaaauga cacacgauac auaucccgcu uugucgcuaa cuaucucgaa     2040

aacgcacuag aauauagaga cgauaguccg aaaaaacgag ucuucaugau uccuggucaa     2100

cuuacagcac aacugagagc aagauggaga uugaauaaag ugagagaaaa cggcgauuua     2160

caucacgcau uagaugccgc uguaguagca gugacagauc aaaaagcaau aaauaauaua     2220

agcaacaucu cacgauauaa ggaauuaaag aacuguaaag auguaauccc auccauagaa     2280

uaucacgcag acgaagaaac uggagaagua uauuucgaag aaguuaaaga cacaagguuc     2340

ccaaugccau ggucaggauu cgaucuagaa uuacaaaaaa ggcucgaauc agaaaaucca     2400

agagaagaau uuuacaaucu uuuaagugac aaacgauacc uaggaugguu uaacuacgaa     2460

gagggcuuua ucgagaaauu acgaccugua uucguaagcc ggaugcccaa uagaggaguc     2520

aaaggccaag cccaccaaga aacuauaaga aguucaaaaa aaauauccaa ucaaauugcu     2580

guaagcaaga aaccacucaa uaguauaaaa cuuaaagauc uggagaaaau gcaaggacga     2640

gauacagaca gaaaauugua ugaagcauua aaaaacagac uagaagagua cgacgauaag     2700

ccagagaaag cauuugcgga accauucuac aagcccacaa acagcgggaa acgaggaccu     2760

uuaguuaggg guauaaaagu cgaagaaaaa cagaacgucg gagucuaugu aaauggagga     2820

caagcaagca auggaucaau gguacgaauc gauguauuca ggaaaaacgg caaauucuac     2880

accguaccca uauacguaca ucaaacacuu cuaaaggaac uaccaaauag agccauaaac     2940

ggcaaaccgu acaaagauug ggacuuaaua gacgguucau ucgaauuuuu auauagcuuu     3000

uauccaaacg accucauuga aauagaauuc ggaaagucaa aaucgauaaa aaacgacaac     3060

aaacuaacaa aaacagaaau accagaaguc aacuuaucag agguauuagg auauuauaga     3120

ggcauggaca caagcacagg agcagcuaca auugauacuc aagauggcaa aauccaaaug     3180

agaaucggua ucaaaacggu aaagaacaua aaaaaauacc agguagacgu acucgggaau     3240

guauacaaag uuaaaagaga aaagcggcag acuuucuga                            3279


<210>  5064
<211>  3285
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5064
augaaaucaa cauuggcuau agcaucuauu ucaagucuag gauggguaau cgaauuagga       60

gaaaauagaa uaaugagacu cggacuagau auugggucaa ccaguauagg gugguggauc      120

uacgagacaa ccgacggaca aauaacaagu guaauagaug gaggugugag aauuuucuca      180

gauggaagag aucccaaauc aaaagguagc uuagcaguag accgaagagu ugcuagagca      240

caaagaagaa ggagagaccg auauuugcgg cgaaaagcag cauuaaugaa gaggauggca      300

gaaguaggac ugaugccuag cgacccaaaa caagcaaaac aauuagaagc acuugacccu      360

uaugaguuaa gagcaagagg acuugacgag acccuugaau ugacagaacu uggacgcgca      420

cuauuucauu uaaaucaaag aagaggauuu aaaucaaaca gaaaaaccga cgcaggagau      480

aacgaaucgg gaaaaauaau ugaugcaaca gcuagacuag auugggcaau gagagaucac      540

cgggcucgga cauaugguga auuuuuacac augcgacguc aaaaagcaga cacaccucgu      600

acaacuccag caguaagaac aagguuaaca auagcagcaa gagauggugc agaaaaagaa      660

gagucggggu acgauuucua cccugacagg caacaccuga gggaugaguu ugaugcauua      720

ugggaagcac aagcugaaua ucaugggguu uuaacagaag auuuaagaga ugaaauagga      780

acuauuauau uucaucaaag accgcuaaaa acuccaaaag uuggcaaaug ucuuuuuacu      840

aacgaagaac guguggcgag ugcccacccc uugaaucaga gacgaauuuu aguugagacu      900

guaaacgccc uaagaguagc ccaauuagga gaaguagcac gagcccuuac acuagaggaa      960

agagauacua uauuacaugc auuggauaac aaagcacaca caaaaucacu aagcggaaug     1020

agcauaaaau uaaaagcguu agggaaagua auaaaauuac gaucagggca auauuucacc     1080

cuagaaacag cgaacagaga ugcaauagca ugugauccug uaagagcuuc uuuaagcaaa     1140

ccggacagaa uaggaucaua uuggaguaca cucgaucaua acgcccagug ggaaguggua     1200

aaccgaauaa gggcagugca aucugaugca gaucacagag aauugguagc auggcuagua     1260

ucccaacaua gacucgccgc agagcaugcu gaaggagcag cauaugcacc auuacccgau     1320

gguuauucaa gauuagguau aacagcaacu aaaagaauac uugcugccuu agaggcaaac     1380

gucauuccgu acaguaaagc aguagagagu ugugguuggc accauucaga ugagagaacc     1440

ggagaaguuc uagaccgcuu accguauuau ggagagauac uagacagaca cguaauucca     1500

ggaacguacg augacucgga cgacgauauu acaagauuug gaagaauaac caauccuaca     1560

guacauauag gacugaauca gcuuaggcga uuagucaaua gaauaauaga aguacauggg     1620

aaacccgacg aaauaguugu agaacuagcg agagaccuua aacuaagcca agaccaaaaa     1680

gcagaugucg aaagaacuau uaaaaagaau acucaagcag cgauaagaag aggagaaaaa     1740

cuagaagaga ugggaguacc aaauacagga ggaaauagaa ugguauuaag guuaugggaa     1800

gacuuaaacg augacgaugu aaugaagaga uuuuguccau auacaggaca gcgaauaagu     1860

gcgggaaugu uauuugaugg aucaugugau guugaucaca uucuaccauu uucuagaacu     1920

uuggaugacg gguucacaaa uaaaacccuc ugucuaaaag aagcaaauag aaagaaaaga     1980

aauuacgccc cucaugaagc agcagagaaa gggcucuuag auagggaugc aauggauucu     2040

guagcaaaau aucuacccga caauaaggcg uggagauuug cgccggaugc aauagaacga     2100

uuugaaggcg aaagggacuu cacagcuaga gcacuaaaag acacacaaua ucucagccga     2160

uuagcaagaa ccuauuuaga cgccuuauau gauggaggag augauaaaag ucauguuugg     2220

guaguaccug gucgauuaac cgaaaugcuu agaagacauu ggggacuuaa uggacuugaa     2280

gcauuagcag auagugacga gaaaacagua aaacggaaaa auagaacuga ucauagacau     2340

caugcuauag augcugcagu uguagcggcu acagauagau cauuaaucca gaaaauaucu     2400

aagauagcag caucagauga acaagcagga gcagaagaua cauugaaguc aacuccacca     2460

ccaugggaua gcuuuaggga cgauauagca gcccaacuac agaauauaac agugucgcau     2520

agggcagacc acggcagaac agauagucua gcaaaagggc guggagaaga uacaacaagu     2580

ggagcacuac acgaagcaac agcauuagga auaaucgaug aaacccaugu aacagucaga     2640

auaccaauug aaucguuaaa aagcggacac cucaucgaag gaucuagaaa uggauauaua     2700

agagacccau accuaagaga ccaauuaaca uuagcuacua aaaacggaag ugauaaagau     2760

uucacaacag uccuacaaaa auuuuucgau cguaguaaaa gauacggagg gaccagaaga     2820

guacgcauaa uuaaaacacu gcaagacacu gcccgcauau cuguaccaca caaugcacca     2880

auaaaagccu acaaaggagg caguaaccau gcuuucgaaa ucuggaggcu gccaaacgga     2940

aaaauugaaa gccaagucau uacuacuugg gaacaucaca aucuaacaga accgaaacgu     3000

ccgcacccug cugcaaaaag aaucauccga cuacacaaaa cagauauggu cgcaauagaa     3060

agggauggua aaaccguuau auguuuugug cagaaaacag auccaaguaa cggggcguuu     3120

uugguaccac auuucgaagc gaacgcagac ggaagaaaac ggaaaaacga accuguagaa     3180

ugguaccaaa ugucagcaaa cucccuaaua aaagcagauu ugagaagagu agggguagau     3240

gaaaugggaa gaguacgaga uccaggacca ccacccauua gauga                     3285


<210>  5065
<211>  3285
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5065
augaaauaca gagugggcuu ugaccuggga acuaacagcu uaggaugggc cguuguacgg       60

cuaaaugcca guggggaacc agauagaaua gaaguugcug gcagcagaau auuuucugac      120

ggaagggaac caaaaagugg ugcgaguuua gcaggggcaa gacgagaugc aagagcaaug      180

aggaggcgga gagauagguu uaaacaaaga caagcggcau uaauaaaaua ucucacacaa      240

gcaggauucu uuccagccga ugaaucagag agaaaagcuc uaagcgcacu agauccauau      300

gaacuacgag caagagcauu agaugaagca uuaccacuag cgcaucuugg gagagcauua      360

uuccauuuaa aucaaaggag aggauucaag aguaauagaa agacagaucg aggaaagaau      420

gacgauacug guaaaauaag aauuggggua gcgcgacuau acgagcaaau gagagaugcu      480

gacgcacgaa cacucggaga guucuuacau aaaaggagag cugcugccag ugauccaaac      540

gcaauaccac augugaggac ucgaauaguu acacuagcgg cagaggaugg uaaagcaagc      600

gauguauaug auuuuuaucc agauagacaa acucuugaag aagaauuuga agcuauaugg      660

gaagcacaaa cgagucacca uccggaagcg cugaccgagg agcguagagc acaauucuac      720

gaaaucauau uucaucagag accuuuaaag caaccgaaga uaggauuuug uacgcuucua      780

ccaccagaga aaagauuacc aaaggcccac ccauuauuuc aaagaagaag acuacuagaa      840

gaaguuaacg cauugagaau agucuugcca gggaaagaaa caagagcacu agaaaaagca      900

gaaagagaug cauuauuaag caaacuuaau caaagaaaga aagcagcgua ugaaagucua      960

agaaaagcau uaaaauuaga cccagaugcu agguucaaua aagaaagcga caacagagaa     1020

gacaugauag gggacgaaau agcagcugaa auggcacaua aauccagauu cgggaaagug     1080

uggcuaggaa ugagugcgga agcacaggcc caaguuauag aaagauuaag agguuuagaa     1140

uccgaugcag augcagauga cuuugaagca uggcuacggg augaucaugg gcuagcgcaa     1200

gaacaagcga aggcaguaac agcggccaau uuaccagaag gcuacggaag auuuggggaa     1260

acagcaacga ggaaacuaau ugcagcuuug gaacaagaag uaauaguaua uucagaagcu     1320

gugcaaaggg caggucucgg ucaccacuca gacuuacggg auggccaagu guggacagac     1380

aagaaaggaa auccagcccu ccccuauuau ggugcaguau uagaaagaca uauaguaccu     1440

ggaacugcag auccuaacga aacagacgac gcauuaagaa uaggcagacu aacaaacccc     1500

acaguacaca uaggauuaaa ucaacuuaga agaguuguaa augcauuaau gagaagguac     1560

ggacaaccgc aggaaaucgc aauugaauua gcaagagaac uaaaacuauc agacgauagg     1620

aagaaagaaa uaaauacgag gaauagagaa aaucgaaaag cagcagagaa aagaucucag     1680

aaauuacgaa auauaggaca agcggauaaa ggagguaaua gggccuuacu aaaacuaugg     1740

gaggaacuca auccagaaaa uguauuagau agacgaugug uauacacagg gagaguaaua     1800

aguaucgaua ugcuauucag uggagcggcg gaaguagacc acauacuucc uuuuagugua     1860

ucucuagaug auaguaacgg caacagaaua uuaugccuaa gagaaguaaa uagaauaaaa     1920

cgacaaagaa cuccauggga agcacgagau gaccuacgag cacacuuugg cccagaugca     1980

gguugggaag cgauagccgc aagagcagcu cggcugccua gggagaaaag auggagauuu     2040

gaaccagacg cagcaaauag auuugaugca caaggugggu uccuugcacg ccaucugacc     2100

gauacacaac auuugaguag acuggcaaga agcuacuuaa gugcacucua ccccgaaacu     2160

ggagaaggcu caggacaugu uuggguuucu ccaggaaggc uuacggaaau gcuuaggaga     2220

gccuggucau uaaaccauca ucugccagac cacaauauag caggagcagc aaaucagccu     2280

aaaaacagaa aagaucacag acaucaugca auagaugcga ucguuguggc auuggccgau     2340

agagcaaugu uaaauagaau agccagagaa ucaggcaaag aaggacauga agcggcgggc     2400

cgguuaguug cgaguauucc ugaaccgugg gaaggcuuua gugaugaagu agggagagca     2460

guacgagcua uaacuguuag ccauagaccg gaucacggga ccgcuaguaa agcaggcaga     2520

gcuaaagaca aagaugcaac agcaggaaga uuacauaacg aaacagcaua cggauugaca     2580

ggagaaaaag augccagagg acuagauaua guaguuacac gaaaaccauu aagugcuuuc     2640

aagaaaagug cugacuuaga cgcaaucaga gaugaugauu uaaaacaaaa acugaaagcc     2700

ugggcagcag guaaagaagg cggagcauuc gaacaagcua ugcguaguuu cggagaucca     2760

gagagaggac cuagaaguca cccaggacua agaaggauaa ggguuacuga accauuaucc     2820

guaaucccaa uuagagaucg cgauggaagg ccuuauaaag cauauaaagg ggacucaaau     2880

uauagauuug auauauggga auugaaaaca gguaaaugga aagacgaagu gguaaguaug     2940

uuugaggcac auagaccaaa uuggacaagu ccaaucagag ccgggaaccc aacggcuaga     3000

aaaguauuaa gcguaagaca aaacgaugua auugcaauag agcgagaaaa uggcccagaa     3060

cucaugaggg ugguuaaauu uagugcagga ucacuagccc uagcaccccc acaggaagcc     3120

ggaucauuaa aagcccgaga cgcugacaag aaugaucccu uuaaauaugu guacggcagc     3180

ccuagcaguu uacaaagaug gaaagcuaga caaguaagaa uagaugaacu aggacgugug     3240

uucgauccug gguuugcagu acgaaguaga cgaucaagug aauga                     3285


<210>  5066
<211>  3291
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5066
augaaaaaua uauuuucauu cgacauagga acaaauucaa uagguugggc uguauuagca       60

cuugauggaa auggagaccc aguaagaauc gccgacgcag gaguaagaau auucuccgau      120

ggacgagauc caaaaagcaa agcaucuuug gcagaagcac gaagaauggc aagagcaaug      180

agucgaagaa gagaucgaac auugagacgc aaacgggcca cuuuaagaac uuuagcagag      240

auaggguuaa ugccggacac gagcuccgca aggcaggcac aauuaagaga gacaucggau      300

ccaaaaagug ggaguggaca auugacacga aacccauaug cauuacgagc auuggcguua      360

gaagagaaac uaccacuauu ucauauaggu agagcguugu accauuuagg acaacggaga      420

ggauuucaau cuaauaggaa aacagauaga aaagacaacg auaaaggaaa aauugcacuc      480

ggaauagaag aacuacacgc cgcaaugagg caggauggug cccaaacaua cggacaaugg      540

cucgcucgaa gacacgcacu aggucaagcc gucagacuca ggagcggagc guuagaccuu      600

gaaggagaag gauaugcuuu uuacccugaa aggaaacuac uagaagauga guuucaucga      660

auaugggagg cucaagcaag acauuaucca gauauauuaa caccagaaag aagagaccau      720

cuauuuacag uuauguucuu ucaaaggccu cuaaagaaac cuaaaguagg aaaauguagu      780

uuuaauaacc aagaagaaag guuaucaaag gcacacccgc uauuucagga auuuagauua      840

uauaaagaag uaaacgaacu agaaauaguc gaaccagauc uaacucacaa aaagcuaaca      900

cuagaccaaa gaaaugcauu aguaaagaaa uuacaaguaa guagagaggc aucguuugcc      960

gcccucagga aaguguuaaa acuuagacca gaccaacgac uaaauaaaga aucagaaagc     1020

agaaccaaau uaaaaggaaa ugaaguguau gcaguacuau cggauaagac cagauucgga     1080

ccgagauggg gaacauuuuc ccaagcagaa caaugggcua uaauagaacg acuaaaagaa     1140

gaagaagauc cuggacgccu auuugcaugg uuaagaucug cuuucgguau ugaaggagaa     1200

caagcugcug auauagccaa uguacauuua ccugaaggcc auggacgauu aggaaaaaca     1260

gcccuugaag ccaugcuaga agaacucaag gcggagguaa uaacagaagc ugaagcagca     1320

aaacguugug gauacgacca ugcuagacua gggggaggga gugaugaagg agagccagaa     1380

cuuccacccu accaagaaau acuucaacau cgaauaccac ccgguacgaa uaauccugac     1440

gauccauaug acauugaaaa aggaagauuu acaaauccaa caguacauau agcacuuaau     1500

caauuacguc auguaaugaa uacacucaua agaagauacg gaaaaccugc acaaauugca     1560

guugaauuag cgcgagauuu aaaauuaagc gaaagacaaa aggaagaagu gaacagaaca     1620

auagcaagaa acaccagaga ugcagaaaga cgcggaaaaa cgcuagaaga gaauagaaua     1680

ccaaacacag gauauaaucg cacauuauua agacuauggg aggaacuaaa cccaggaaaa     1740

guagaagaca gauguuguau uuauacaggu aaaccuauau caauaagcau gcuauucucc     1800

ggagaaguag acauagauca uauauugcca uggucacgca cuuuagauga cagccaugga     1860

aacaaacuau ugugucuaag agaagcgaac aggguaaaga gaaacagagc uccugcagau     1920

guaaccgaau ggagagacaa cuacgacgaa auucucagca gagcagcaag auuaccgcaa     1980

aauaagagag acagguuugc cccagacgca auggaaaggu uugaaaaggc cggagguuuu     2040

cuagcaagac aauugaccga uacacaauac uuaaguagga uggcuuuaga guaccuagcu     2100

uguuuauucc cggcugagga aauggaugga uuuggagaac uaagaagaca uagauauauu     2160

aacgucuguc cagggcgauu aacugaaaug cuuagacgaa acuggggauu aaauuccauc     2220

uuaucagaua gucaauuagg ugaaacagcg caagagaaaa accgaaaaga ucaucgucau     2280

cacgcaaucg augcaauagu uauuggagua acuggacgau cuuuacuaaa uagaauaagu     2340

uacgcugcag gacgccuaga agcacaacaa uuggaaacac ucguaaaaga agcagcggcu     2400

acaaauaaac ccugggacgg uuuuagggaa gacguagcaa aaguaguggg cgggaucauu     2460

guaucacaca aaccagauca ugggacagca ggcagaaaag caaaagaucc ugcaagacaa     2520

caaacagcag gaagguuaca uaaugauacg gcauauggau ugacaggaga aacagacgca     2580

agaggcaaua caauaguugu acggagaaaa ccguuuuuau cccugacguc aaaggacaua     2640

ccgagcauua gggacgaagc ccuuagggca gucuuagaac aacaucuacg aggcauacca     2700

gauggaaaag cauuucaagc cgcauuagac gccuuuagaa aacaaaaagg auuucaccac     2760

uaccauggaa uacgacaugu ucguuugacu gaaccccuaa gugugauugg uauaaaagau     2820

agagauggca aaguuuauaa aggauauaag ggagaugcua aucaacguua cgaugucugg     2880

gaaacuuuag acgguaaaug gcacgccgaa guaguaagca uguucgaugc gcaucaaccu     2940

ggauggcaau caagauucca ugcugaaaac ccuacugcaa gaagaguauu aagauugcaa     3000

caagaugaua ugguagcacu aaaugcuccu gacgguggac gccucuuagu acgaguagua     3060

aaguuccgac aaaacgggca gauuacuuuc gcaggucaca acgaaucugg aaacuugaaa     3120

gcuagagaug cggcaaguaa cgaacaagau ccuuuuaaau auauuagucg aauggcauca     3180

ucuuugaagg auugcggaug ucgucaaguc agaauugaug cuacaggaca aguauuugau     3240

ccggguccac aagauagagc aucaagacua cagaaacaaa guaaacgaug a              3291


<210>  5067
<211>  3294
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5067
augaagaaac aaccucaaag guacauauua ggacuugauc uuggaauagc auccguugga       60

ugggcacuaa uauuaguuga cggagccgaa aauccagucg gacuauuaga uugcggugua      120

agaacauuug aacgcgcaga aguacccaaa acuggcgauu cauuggcguu agcacgaaga      180

caagcaagaa gcguaaggag guuaaucaga agacgagcac aucgacuacu aaggcuaagg      240

cguuuacuca aaagggaagg aauccuuuca gcagcagacu ucaauucaga cgggcuucca      300

aaucuaccua uagacgcaug gcaacuaaga guugcaggac uggaaagaaa auuaacaaau      360

caagaauggg ccgcaguauu acuacauuua guuaaacaua gaggguacuu aucacaaagg      420

aaaagugaaa cgcaaacaga gaauaaagaa uugggacguc uauuagccgg uguagcugaa      480

aauucgaaac uauuaaguga aucuagcgaa caauaccgca cgccagcaga gcuagccgua      540

aaauuauucg ccgaacaauc aggacauuua cgaaauaaag gaggagaaua uacucauaca      600

uuuaauagau uggaucuaca aaaagaacua cauuuacuau uugaaagaca auacgcauua      660

ggcaauccau ucgcuucugc agaguuacaa ucaaaagucg augaauuauu aaugacacaa      720

agaucagcac uacaaggcga agccguacuc aaaauguuag gaaaauguac cuuugagccu      780

ucugaauaua aagcggcuaa aaauacauac agugcagaaa gauucauaug gcuuacaaaa      840

uuaaauaacc uuagaauauu auacaacggu gaagaaagag ccuuggauga aggagacaga      900

aagaaacucc ucgacgagcc auauaagaaa gcaaaauuaa cauacaaaca aguaaggagc      960

auacuaaauc ugcccgaauc cgcaguauuu aaaggacuuc gauacggaaa agauucggac     1020

ggacuaaaag cugagaaugg aacacuaaug gagaugaaag ccuaucauaa aaucagaacc     1080

aucuuggaca aagcaggacu aaaaggagaa ugggaaaaua gaauuagaaa ucccgaaaua     1140

uuagacaaaa ucggcacagc uuucucacua uauaaaacgg acgaagauau acaagcagcg     1200

cuagccgauu uaagauugcc agaaaaugua cuagacgccc uuuuagcagg acugaacuuu     1260

gauaaauuua uacaauuauc uuugaaagcg cuacggaaua uacuuccuuu aauggaaaga     1320

ggaauuagau augaugaagc auguaaagag guuuacggug accacagagc ucuugcagaa     1380

ucuaaaaacc agguauuauu accgccuauu cccgcggacg aaaucaggaa uccagucguu     1440

cuuagaaccc uaacacaagc uagaaaagua auaaacgcaa uaauacgaag auacggauca     1500

ccccagagag uucacauuga aacagcuaga gaguuaggaa agaguaaaca agaaagagac     1560

aaaauagacc gcaaaaacaa agaaaaucag aaugacagaa auagacuugc aucagauauc     1620

agaaaacauu uuaauuuuga acccaccccu aaggacauac uaaaauugag acuauacgaa     1680

cagcaacaug gaaaauguuu auacucggau uuaagagaag aaaaaggaga augucuauac     1740

acaggaaaug ucauagaacu ucacagacuu uucgaagaug gauacguuga aauagaccau     1800

gcauuaccau ugaguagaac augggacaau ucucuaaaua acaaaguucu aguacuaaaa     1860

ucauccaauc aaaacaaagg uaauaaaacu ccauacgaau ggcucgaugg cgcaaacaau     1920

aguagaagau ggagagaguu ccaagcaaga guacaagguu gccauuuccc auauguaaag     1980

aaacaacgca uucaaacagc uaaacuugac aguaaagcag aacaggguuu ccucgaaaga     2040

aaccucaacg acaccagaua uauaagcaaa uuuauguguc aauuuauaac ugcaaaccua     2100

cgauuagcag aaacaagcaa ugcucuaaug gaagaaguag caggagaaaa ucccuauugg     2160

aaagaacgaa aaagacaacg aguauucgcu ccgucagguu ccauaauagc acuucuaagg     2220

ggacucuggg gccuaaagaa aguaagagaa gacaaugauc gacaucaugc cuuagaugca     2280

auuguaguag cgugcaguac uguaagcaug caacagaaaa uaacuaaagc aaugcaacga     2340

cgcgaaaccu uagaaacagu agacucagag accggagaag uaaaaacacg aauaccccaa     2400

ccaugggauu ucuucagaca agaggugaug auacgaguau uuagcgauuc uccacgcgaa     2460

gaacuagcag aaaaacucaa uucaagaccu gaagcauuac augauuuugu cagcccuuua     2520

uuuguaagcc gugcucccaa uagaaaaaug acaggucaag gccauuuaga gaccgugaaa     2580

ucagcaaaaa gauuaaguga aaacauaucu gucguacgaa aaccccugac ugcauuaaaa     2640

cuaaaagaua ucgaaaauau aguaggguau cccgacagag aaccugcauu auaucaagcc     2700

uuaagagagc gccuucucgc acauaaagau gaucccguua aggcuuucgc agauaaacca     2760

uucuauaaac cagcgaaaga ugguggccaa gggagccuug uaaaagcugu aagaauaauu     2820

gaaaacacaa aacacggacu aaaaauuaga aacggcauca cugacacaaa cggagauaug     2880

guaagaacag acguguacca gaaaacagau aagaaaggua aaagacaaua cuuuauagug     2940

cccgucuacg cauggcaagu agagaaaaac gaacuaccaa cuguagacug caaaggguac     3000

gaaauagacg acaacuacga auuuugcuuu ucauuauaua aauaugaccu gauagaaaua     3060

caaaauccca augauauaaa ccaagaacug agagcuuauu auauaaacug cgauuccuca     3120

gacgggagau uuuggcuagc cuaucacgac aaaagcgcua aagaacaaca auuucgaaua     3180

aguacacaaa accagccaau aauaaagaaa uaucaaguug acacacuagg gaaagaaaua     3240

agacuaugua aagcagagaa aagaacagcc cuaaacggua aaaagaagaa auga           3294


<210>  5068
<211>  3294
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5068
augggaaagg gugacaucuu auucaguuuu gauauaggua caaacucaau aggauggugu       60

guguuugcac ucucagauaa uggagaccca ugcagaaucc uagaugcaaa cgcuagaaua      120

uuugcagaug gaagagaacc gaaaagugga aauucacuag cggaaggucg cagagcagcc      180

agagcaaugg caagaagacg agacagguac aaacgaagaa gaaaagcagu acuuaggacg      240

uuaacugaau acggacuaau gccagcagau cccaaagcua gacaggcuuu aguagcuaaa      300

acuggagaua gaggaccgaa aagugaagaa gcacuagcag auguauacuc ccuaagggca      360

agagcauuaa uggaaaaacu acccuuacac cauauaggaa gggcauuauu ucauuuaaau      420

caacguagag gcuuuaaaag caacagaaag acagauagga aaagcaauga gaaaggggcg      480

auugagacug gaguaaaucg acuuuuagaa gaaaugcgag acacaggugc acccacauua      540

ggggcuuauu uagcuaaacg aaggucagau ggacgaccgg uccgagucag agccggcgcu      600

ucagagcaag aggccgaaga uggaaaaccc gcgaaaaccg cauacgauuu uuauccaguu      660

cgcagauuau uagaggaaga auuugacgcu auuugggaaa aacaauacuc auaucaccca      720

gaaguccuaa cagaagaaag aaggggucau uuauuccggg uaauguuuca ucaacgaccu      780

cuaaaaccag caauaguagg acgcuguuca uuuaauagug cagaaacaag auuagcaaaa      840

gcucauccgu uauuccaaga guucagauug uauaaagaag ucaaugaauu ggaaguugua      900

augcccgauc aaucgcacag gaaacuaacg cuacccgaaa gagaucgucu cguauuaaaa      960

cuccguggac agcgaaaagc gacauacgcg gcucuaagaa aagaacuacg acuacgugac     1020

uugcgauuua auaaagaaac agagaauagg acugauauga ugggugauga aguaacagcu     1080

gcauuagcag acaaagccgu auucggaaau cgauggucag gauuuucaac agacgaaaaa     1140

uggagaauag uagaaagacu aagggaggaa gaagacccuu uugcuuuaca cgaauggcuc     1200

guuagugaac acggcuuaag ugacgccgaa gcagaacaag cagcaaaagu gcaucuaccg     1260

gacggauaug gaagauuagg uccuacugca gugucgacua uguuagagga auuaaaagcu     1320

gacguuauaa cggaagcaga cgcagcaaga uuagcuggau acgaucacuc acuugcuaga     1380

gcagacgaag auggguuaga uagacucccu aaguaccaag agguucuuga aagacgaaua     1440

ccuccgggaa ccaaugaccc ggaugaucca uacgacaucg ccaaagguag gauaacaaau     1500

ccaacagugc auauagcucu aaaccaauuc agacauguag uaaaugccuu agcucgacga     1560

uucgguaaac cagaucaaau cgccauagaa cuagcaagag aauuaaaguu aaaugacagg     1620

caaaaagacg aaguaaaucg uguuauagga agaaacacac gcgcugcugc cuuacguuca     1680

caaaaacuaa gagauauugg ucagaaagau aauggauaua auagagcucu acuuaaauua     1740

ugggaagaac uuaacccaga caaaccagaa gacagagucu gcguauacac aggaaggcca     1800

auuugcauaa gcauguuauu uucaggcgaa guagacguag aucauauuuu gccauggagc     1860

agaacuaugg augauucaca ggcaaacaga aucuuaugcg uaguugaagc aaacagggua     1920

aaggcaaaua gagcacccgc agaaguagca gaauggggag gaagguauga cgaaauuuua     1980

gaaagagcgg caagguuacc gagaaauaaa agauggcggu ucgcuagaga cgccauggaa     2040

aaauauaagg aaaaugaagg auucuuggcg cgacaauuaa cagacacaca auaucuuagc     2100

agauuagcga gagaauaugc ggaggcuuua uacccaacag aagaaccuga uagauugggg     2160

gaauuaagac gucgaaacca cguaagagua auuccuggua gacuaacaga gaugcuacgg     2220

agagguuggg guuuaaaugc ucuauugccc gaucauaauu ucggagcagg acaagccaaa     2280

aacagaaaag accaucgaca ucaugcaaua gacgcugcag uagucggagu aacaacacga     2340

ggucuauuac agaggauaag uacggaagcu ggauuaagag aaaaauuagc acuugaagag     2400

acaauagcgg auaucccaau gccguggcca ggauuuagag aggaacugaa agccgccaua     2460

gaccgggcug uagucagcca uaaaccagac cacgggacgu ugccgaaacc uggugaacau     2520

gggagaacag caggucaauu acacaacgac acggcauacg gaauaacagg agaaacagac     2580

gcaaggggaa acccaauagu aguaagaaga aaaccauuuc uuucauugac ggacaaagau     2640

auaccuagua uaagggacac agaauuaaga ggaagaguau acucagcaac aagcggacua     2700

ucuggaagag aacuauccgc agcccuagau uauuuucgaa aaaccgacaa acaauuccgg     2760

gggauaagac acguacgugu aacagaaagu cuuuccguaa uaccgauaag agauagagau     2820

ggaaauauau acaaagggua caagggugac gcaaacuuuc gauaugaugu uugggaguua     2880

ccagauggca aguggaaagc cgauguaguu acuauguuug augcccaucg gccagacaga     2940

gccgcagaag aacgacgucc acacccagca gccagaaaag uauugagucu aaaacaaaau     3000

gacaugguag cuugugaaca cccaaaagau ggauacacaa uuggccgagu uguuaaauuu     3060

aacacugcag gacaaauaua cuuugccgcc cacaaugaag guggaagucu aaaagcaagg     3120

gacgcagauc gagcagaccc auuuaaauau uucucaaaga gugcuagugg cuuaaaagaa     3180

auagcagcaa gacaaguaag aaucgaugca acuggacagg uacuagaccc uggcccacaa     3240

gauagagaau caagauuagc gagaaacaag agacuugaaa gagaaagagc auga           3294


<210>  5069
<211>  3303
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5069
augaaguaua caauaggacu agacaucggg acaagcagug uagguugggg cguaauagau       60

accgaaagga accgaaucca agaucuagga guaagauuau ucgaaagugc agaaaaccaa      120

aaagauggag aaucacuagc aaaaccaaga agggacgcaa gaagcaugag gaggagacua      180

gcucgacgag ggagucgucu cgaaagagua aagagcauau ucgaaaaagc caaauuucuu      240

acuagaggac aaauggggau aacacacgcu cgcccaaaug aucccuacag auuacgagcg      300

gaaggcuuag aucgucuucu aucaaacgaa gaucuauuca ucgcaguaua ccaucucgca      360

aaaagaagag guuacaaauc caacagaaag aacguacuag aaggagaugg agaagaugua      420

cgcgaaggaa aagccguuuu ggauggagua gcacaaaaua aaaaacugau ggcauuccga      480

ggcuacagaa caguaggaga aauguuuuuu aaggauagug uuuucacaga agcgaaaaga      540

aacaaagcag gaaguuacaa acacugcgua cuaagagaaa uguuagaaga ugaacuggga      600

aaaauacugc augcucaaag gagucucgga aacaccuuag uuacagauuc auucaucucu      660

aagcuucucg aaaaaaaaac aggaguguuc guauaccaga gaccuuuugc aucagguaau      720

caaauacuca aacucguugg accaugcaca uucgaaccag aagaaauacg agcagcaaag      780

gcaacauacu cuuuucaaua cuuuaacguc cuacaacguu uaaacaaucu aacaauaaaa      840

aacacagaca caggaacaga aaggaaauug accagugaag aaagagcaaa aaugauagcc      900

uucauuuucu cacauaaaga ugugcaauac gcacagauaa gacucgaacu caaacuaagu      960

gauguugaua gguucaauau gguuaauuau uucguucaaa gaaaagaacu agaaacaaag     1020

acagcagaag aacuaagaag ggaaaucgaa aaaaaaacca aauuuccaag uuuaucggac     1080

uacuacacaa uucgaaagac aguaagcgag auagauucag cguuuuggau ccaaauagaa     1140

aaacaacacg augucauaga cgcgauaggg gaaauauuca cauuguacaa aacagaugaa     1200

gacauaauag cacaacuaaa cacaagaauc ccuuccauac ccagaccagu aguaucagcu     1260

uuacuuggac uaagcuuuac aaaauuuggc caccuuaguu uaaaagcucu aagaaaaauu     1320

auaccacauu uagaaaacgg agacacauau gacguagcuu gcgcuaaagc ugacccaaaa     1380

uacacagauc gagcaaaaac aagagcaaga aagcuaagac cccuagauaa agaagaccau     1440

ucaauaacaa acccaguagc cagaagagcu auagcacaaa cgaucaaagu aguaaaugcc     1500

auaauagaca aauacgauuc uccgagugaa guacacaucg aauuaggacg ggauuuagca     1560

aaaaauuuca aagagagaau gaagauacaa aaagaacaaa aacaaaauca agaaggcaac     1620

gaaagagcua uugcaaaaau acaugaguug uauaaauuag aaaacccacg aggacaagac     1680

auaauaaaau uccgauuaug gaacgaacaa aacggacaau gcauguauuc aggcgaaaga     1740

auagaugaaa uuagacuauu cgacaacggu uacacugaaa uagaucauau aauccccuuu     1800

ucgagaaguu uuaacgauag cuacucaaac aagguacuug uauuaaaaau gagaaaucaa     1860

gaaaaaauga acaaaacgcc auacgaauau auggggggag acgcagacac cuggcaaagg     1920

uucgaaaacu uagugaacuc aaugcauaau auaggaccaa gaaaaaaaca aaaccuccuc     1980

cuaaaaaaau acgucgcaga cgaucucaca cuaagaacau uaaaugacac acgauuuaua     2040

gcuagauaua ugaaaaauua uauugaaaac accuuacuuu uuacggaagg accacaaaaa     2100

cagagagugu auacaguaaa cggacaagcc acagccuauc ucagaaaacg uuggguacuu     2160

cacaaagaca gaacagaaaa caaccgucac cacgcacaag acgcagucgu gguagcagua     2220

gcaaccccaa gucuaguaca gggaauaaug cgacaugcaa aaaugggaga aauaguggau     2280

uacuuacauu cacaaaaauu ugcaccuaac guaacugaua uagaaacugg caaaccauuu     2340

gaauccgcag acauccaauc agccaaagaa agaauaauag caaaacauga acaucgauua     2400

agaauacccg agccuuggga gggauucuca gacgaacuag acgcaaggau gggagacgau     2460

gucguugaaa aacugucauu aagaaaaagc aacauucaug gguacaaaac aacaagcgac     2520

uucagucaga uacacccaau uuuuguaagc agaaugccaa aaagaaagac aacuggaaga     2580

gcccaucaag aaacauuaag aaguccuagg agguucaaag aguuucaaga aaguguugug     2640

cguacccccc uaucaagcau uaaucuuaag aaacuagaag aaaugagugg aaaaaacucg     2700

gacaaacuac uauacgacau acuaagaaaa agauuacuug cacauggaga ugauccuaaa     2760

aaggccuuua cagaaccagu ucacaaacca agaaaggacg gaacccaagg cccuuuggua     2820

agaucaauaa agaugguaag cgaagggcaa agaaguggga uucuaguaaa uaacgaacaa     2880

gcauuaguug accaagacag caugauaaga auagauauau uuagcaaacc caacaaaaaa     2940

ggaaaagaug aauauuacau aauucccguu uacgcauacc auuuugccca aaaagaguua     3000

ccaaauaaag ucauaacagc gaaagcagaa gcagacugga gaaccauaga cgacacguuc     3060

gccuuugaau ucucucuaua uccaaacgac cugguaaaac uaacuaaagg agacgauauc     3120

agauucggcu acuauucagg auguaauagg gcacuagcag cuauacauau auugccccau     3180

gaccaaaaca ugccgcucgu uaauaauggc auuaaaaccu uagauaagau agacaaauau     3240

guagucgaua uacucgggga auaucacaaa guuggcaaag aaacgagaca acagauacaa     3300

uga                                                                   3303


<210>  5070
<211>  3303
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5070
augggagcga caaucgugac ggauauaagu agaucagcau uugcuuuuga uauaggcaca       60

aaaaguauag gaagugcagu auuaagacuu gacgauaggg gagaaccgau aggaauaaua      120

aacgccgcgg uaagaauaua ugcagaugga agagaaccaa aaagcggcac aaguuuagca      180

gaaggacgaa gauuagcccg aggaaugagu cguagaagag aucgguauaa aaggaggagg      240

aggacucuau uaaacacccu aacagaguuc ggauuaaugc caccaacaga cgaagcucga      300

aaagcacuau uagcagaaac caacgauaga agauuuggag ccguggcagg aaauguauac      360

gcacuacgag cucgagcacu agaugaaaaa cucccccuac aucauuuagg aagggcguua      420

uuucaucuga aucaaagaag aggauucaaa agcaauagaa aaacagauaa aagagauaau      480

gaccagggaa aaauagcaag cgguauuuca cgcuugggac aaaaacuaga ugaagcugga      540

gccagaacuu uaggagaguu ccuucauaga caacauagug cuggaaaaug gguaagaguu      600

aggccaagcc ggguuccgag uaaagaugau aaagaggaag acggauaugg guuuuaucca      660

gaacgaucuu uucuagaacg agaauuugca gcaauaugga aagcacaagc cgcauucuac      720

ccagagcucc uaacagaaga acguggagac cauuugcacc guguaauguu cuuucaaaga      780

ccccuaaaga gaccgaaggu aggaaaaugc ucauuuuacc cggaugaaga caggcuaccc      840

aaagcacauc cacuguuuca agaguuucgc uuaguaaaag aguuaaauga acucgaagua      900

guacaccgag gugacaggcg agaaaaguua acacuugaac agcgagacac acuguuuaau      960

caacuaagaa cacaaagguc uuccgcguuc acugcacuaa gaaaaacauu aaaagcucca     1020

acagggacaa gguuuaauaa agaaucggac gcaagagaca aguuaaaagg agaugaaguu     1080

gcugcagaaa ugucucauaa agacagauuu ggucaggcuu ggauaggaau gccgcuugca     1140

gaacgauggg caguuaucga aauguuauua gauacagagg auccacuaga gguaaaagca     1200

uggcuagaag aacauuacgg cuuugcagcu gauagaauag augcaauugc agccgcuaaa     1260

cuuccaccag gauauggaag auuaggugaa acagcucuaa gggauauguu agaugaauua     1320

aagaauggaa cagucaaaga ugacucagga agggauguag uauuaacaga aagacaagca     1380

auggacaaua uauggaagga caacccugcu uuacaaaggg uggucgcaga cgcucgugaa     1440

cugccaccgu accaagaagu uuuaggucga cguauuccgc cagggacugg aaaaccagaa     1500

gacccuuaug acgugcaaaa aggcagauua accaacccga ccguucauau aggauuaaac     1560

caauuaaaaa gaguaguaaa ugcauuagua agaaggcaug gacguccaga ccaaauagca     1620

cuggaauuag caagagaguu aaaacuaaac gaagaacaaa aagccgaaaa agauagggaa     1680

auagcgagga auacaaaagc ggcacaggca cgaagugaaa aauuaagaca acuagauaua     1740

gacgauaacg guuacaaucg uguucuguua aaauuguggg aggacuuaaa uuuaagacag     1800

ccagaaaaca gagaauguau auauugcgga aaggcaauag gaauagguac auuauucuca     1860

aaucaagucg acauugauca uaucuuacca uggucaaaaa cccuugacga uucacaagcc     1920

aacagaauag uaugccaugu agaaugcaau agacaaaaag gaaaucacgc gccggcugaa     1980

gugccacaau ggcacgaucg auacgaugac auacuagcuc gagcugaaag auuaccuaga     2040

aauaaagccu ggagguuugg ccgagaagcg augaagaagu uuaaagauga ugaaucauuu     2100

uuggccaggc aacuaacuga cacacaauau uugucccgaa uggcucacga auauguugcu     2160

gcacuuuacc cagacgagga gccugaugaa uggggaguau uuacgagacg aaaccacgua     2220

agaguaauac caggaagaau gacagagaug uuaagaaggc aauggggauu aaaugccaua     2280

aucucagacc augaccagaa aaauagaaau gaucacagac aucacgcuau agacgcuaua     2340

gucguuggag uuacaacaag gagacuauua cagagaaucg cuacugcggc gggaggaaga     2400

gaggcauuag cguuggagga aacaauaggu aagaucgaac cuccauggcc cggauucuau     2460

gacgauguga aagaagcuau agacaggauu guugucagcc auaaaccaga ccaugguuca     2520

uuaccaaaac cuggagaacc ggcacgaacu gcuggccaau uacauaacga cacagcauac     2580

ggauuuacag gagaggaacg aaaaggugua ccaaucaugg ugcauagagu accauuugcc     2640

accuuaacag aaaaagauau accaucaaua agggacaggc aguuagcgga agcacuguac     2700

ggggcaauag cugggauaag uggaaaagcu gaauuggcag ccgcucuagc acgauuuuca     2760

gcaagcggac cuuauaaagg aauaagacau guuagaguag ucgaaccugu cuccguaauu     2820

gcaauaaaag acagggaugg aacaccauac aaagguuaca aaggagaugc aaacuacaga     2880

uacgacguuu gggaacuacc gaauggaaaa uggacucaug aaguaguauc cauguuugau     2940

gcccauagac ccgacuggca gucugaaaua agaaauaaaa auccagcugc aagaaaaguu     3000

cuaucauuac aucagaauga uaugguagcc uacgaacacc cucaagacgg uuauaccaua     3060

ggcagagucg uuaaauuugg aucuggggga caaauggcau uagcaggaaa uagagaagga     3120

ggagcuuuga aaaguagaga ugcugauccu gaagacagau uuagauacuu uuacaaauca     3180

ggaggagcca ugagagacgc aagacugaga caagucagag uagaugagac aggacgcguu     3240

uuugauccgg guccucaaga uagagaaagu agaaaugcca ggaaacaauc cgcagccggg     3300

uga                                                                   3303


<210>  5071
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5071
augaaaaccu uaggccuuga ucuugguacu aauucguugg gaugggcaau uguagaaaca       60

agagacgaac ccacaaaaua uucagaagga ucaauaguag cuucaggagu aagaauauuc      120

ucacaaucag aaauggcagg aagagauccc caaucgaaag ccucauuagc aguagcaaga      180

agacaggcaa ggggaucccg aagacgaagg gaccguuauc ugaaaagacg acaacggcug      240

gucgauuugc uuguagaccu uggacuaaug ccagcagaua aggcagcaag ggcagaauua      300

guuaaaaauc acggagacgg aaagacagga gauuugaguu caagcguaua ugcauuaaga      360

gcucgagccc uugaugaagc auuaaguccg uaugaaauag ggagagcaau uuuccacuua      420

aaccagcgaa gaggauuuaa aaguaaucga aaagcagaua guaacgaucc agaacaagga      480

gugaucagcg aaggaaugag ggcauuggau gagaaaaugg cggaagcugg cgcaagaaca      540

aucggagaau gguuacauau gaggagauua caaggcuuac ccguaagggc aagaauagca      600

ccagauggag aagguuacgc uuuuuaucca ucacgagggg cguuagaaag agaauuugac      660

cgaauaaugg cacgacagac acuauuucau acugaccugu uaacagacac ugccauacga      720

gaauuaagag aagucguauu ucaucaaaga cccuuacgac caguaaaacc gggaaaaugu      780

agcuacaauc cauuagaaga aaggcuagca aaagcacacc cauuauucca aaaauuuagg      840

cuucuaaaag aacugaacga auuggaaaua guuggugaag aucaaaagca caucaaauua      900

acaccggaac aacgaaaugc uuuagcguua caacuacgaa cuucauuaac aaagcaagga      960

aaaguaccuu uuaguaaacu gaggagcaca cuaaaauuag ggagagaagu aagauuuaau     1020

aaggaaacag acaacagauc agauuuagaa ggagauguaa uauauuucag gaugucuagg     1080

ccagaauguu ucggaaaccg uugggcagau uuaccaguug aaaugcaagc ggcaauuaca     1140

gaaagauugc gaacagaacc agauuauaaa acacuaauga auugguuaaa agccgaaacg     1200

ggucuagaug aaguacacgc gagagcugua gcugauacac cagugccuga cgguuucgga     1260

agauuaggac aaucugcauu aacagcauug auagaugcau uggaaaauga agugggugag     1320

ggagguuuug uaauuacuga ggcagaagca gcaaaaaggg uauacggacg uacgaacagu     1380

gaagccgauc cagauagaaa aggcguugau gaauuaccca aguaccaaga agucuuggca     1440

agacacauuc ccccugguac cggugagcca gaugauccgu acgaugauuu uaugggaaga     1500

auaaccaauc caacagugca uauagccuua aaucaacuaa ggagaguggu aaaugcacuu     1560

auaagaacau auggaaaacc cgauagaauc gcaauagagg uagggagaga ccuuaaaaug     1620

aaugauagag aaagagacga aguuaauaga cguauaggac guaauacaaa ggcagcuauu     1680

gaaagaggug uaaaauugac agagaccuuu aggcaacuug auacuggaua uaauagguua     1740

agauuagaac uuugggaaga ucucaaucaa aagccugaaa auaggguaug cauauauugc     1800

gggaaagcaa uagcaggcca cauguuguuc aauggagaaa cugacauaga ucauauauug     1860

ccguacucaa aaacacuaga ugauucgaaa gccaacagaa uacuauguug cacgaguugc     1920

aacagggaaa agaaaaauag accacccagu aggguaccuc aauggagaag cagguauggg     1980

gagauucuug cacgagcaac uacacuaccc aaaaacaaac aguggagauu ugcagaagau     2040

gcaaugacaa gguucggaga agaagaggga uuucuagcaa gacagcuuac ggauaugcag     2100

uauauuucua gacuagcacu aacauaucua gcacaucuau acgauuauga agaaguugau     2160

uuggacggua ucuauaaaag acaugcaagg gucagagcau uaccaggccg aaugacagag     2220

auguuacgca gacaaugggg guuaaaugaa cuuuuacacg aucauaaucu uauagguggu     2280

gauguggcua aacccaaaaa uagacuagau caucgccacc augcuauaga ugccaucgua     2340

auugcaugua caucaagggc acuuauccag cgauuagcaa cugcaagcgc ugcagcagaa     2400

gaaagaggag cagagagagu aguugaaagg auagauccac cuuggucagg uuuuagggaa     2460

gauguuaggg cagcaguggg ggcaauaguu gugagccaua aaccugacca cggaacugug     2520

ggcagagagg gguaugcaaa aggucgcgga caaacagcug guaaauuaca uaaugauacg     2580

gccuacggcc acacugggga aaaagacgca ucaggaaaug auauuguagu acgacguaua     2640

gccgugacug auauaagauc cccggcggac auaagaaaaa uaagaacuaa ugcacacgga     2700

cauucagaau uaagagacag gcuauacgaa gcaacaagag ggcucgaagg caaagccuuu     2760

gaacaagccg uuuuagauuu uguuaaaggg gaugcaaaau uuaggggaau uaggcaugua     2820

agaauuacug aaguugccaa uccaguuugg auaacccacg gagauggaag acauaagaaa     2880

ggauauuuac ccggugguaa ugacagauuc gauguauggg aacuugcaga uggaagaugg     2940

gaugcagaag uuguaacgac auuugacgcu cauagaccgg gauuuguccc aagaaugagg     3000

cuagaaaauc acaaugcuag gaaaaucaug ucauuaaaga aaggggauau gauagcauac     3060

aacgauccag uaucgggaca acgaauuauc gcuauaguaa gaaaauucga ccagcgaaau     3120

aaacaguuau auuuagaucc acauaacgaa gcagguaacc uggacagacg ggaaaaagag     3180

aaaacguaua aaccacuucg acccaugccu aauccuuuaa agaaauauag gcccagacaa     3240

gugagaauag acgaaauugg ccaaguuuuc gauccaggcc caugguggga aagacaagau     3300

gguuga                                                                3306


<210>  5072
<211>  3306
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5072
auggaacaaa caagucucga cuuaagccaa gcuaugaaga aagucgcgca accucaaaaa       60

cccuaccguc ucggugucga uguaggaguc gcauccauug gcgcagccgc aguaacagau      120

acagaaauac uaggaauaca ugucaggaca uuugagaaag gugaaauaaa uggcaaaagu      180

ggacgaaaac ccuuaaacgc agauuauaga agacaaaaag gacaaagaaa acgacucagg      240

agaaagaaaa accgccugcu aaguuuacgu aggcuauuca ggagaguagg aaugauagaa      300

agcgcagcaa uugaagcauu uccaauaagc ucgacaucuc cuuggcaauu aagaacagaa      360

ggauuagaca gacgguuaac gaacgaagag ugggcacaag uauuguacca cauugcgaaa      420

cacagaggau uccagucaaa cagaagagua gacauggacg aagcaacaaa auuaagcgau      480

gccgcaggcg auucuccaga aacaaccaca aauacggaga gugauucaga cacuaagaaa      540

auguuagcag gaguagcaga uaacaaaaga cuccuugcug agaaaggaua ccguacaaua      600

ggagaagcug ucgcucguga cccaaaauuu agucauagaa uacgaaacaa agaagaauca      660

uacucacaua caauaggccg agaugagauu aaugcggaac uaaacugccu uuuucaggcc      720

caaaaucauu acggcaaccc acaugcuaca gaagaacuua gcgaacaaau uauagauuua      780

aucaacuggc guagaaaaac aguuaccggu gacgauauaa aaaggauggu aggacgaugc      840

acacuagaac caaaagaaua uagagccgca auacauucug uaagcgcuga agaauuuaga      900

ugguuaucaa cauuaaguag auuaagaauc guaacucaag gagaaucaag accauuaaca      960

aaaugugaau gggacaaagu aguagaucuc ccuuggcggc aagaagcaau cacauauaca     1020

ucaauaagga acgcaauagg uuuacgacac gauauuagau caagauucaa uauaagauac     1080

gaaaccggag gaaagaaaac uaaaaccgau acuaaaaaug aaacaacaaa aggacaaucc     1140

gcagacgcag uaaccaguga aguuaaaacc gcagaaaaag aaacagcguu caaagcuaaa     1200

ggauaucaca ucuuaagaaa agcauacaaa gccuuuggau augaugagca augggaccga     1260

gacagauuca auacugaauu guuagacaca uuagccacag cagucacauu cuauaaagau     1320

gauagcgaaa uccaagccca ccucgaaaca aacgauaccc cagaauacgc aauacagauu     1380

gcucuacgac ucccaacauu gacuaaauuu aguaaauuaa gcaacaaagc aauccuaaaa     1440

auauuaccac acaugagaga acgccaauca uacuccaacg cuugccuacu agcaggcuac     1500

aaaaaccacc auaaacaaua cgaugauaca ccacaaaaug gaaaaauacc aagauccgcc     1560

auagaacaua uuaaaaauac aguuguauac aggagcuuga accaagcccg uaggguaaua     1620

aaugcaaugg uaaaagaaua uggagcaccc caagaagucc auauagagcu ugccagagau     1680

auggagaaaa gugcgaaaaa gaaagaugaa auagaccuuu ggaugaagaa acgcaaaaag     1740

aaaaaugacg aauugaggga gagauucggu aaaaaccuau cuagagacga auuauuaaaa     1800

uacaaacuau acgaagaaca aaauggacaa ugugcauauu cacuccaacc gauacaauua     1860

ggacgauuac uugaaccgaa uuacgucgaa guagaccaug cacuaccuag aagcagauca     1920

auggauaauu cauauaacaa uaaaguccua guacuaacaa aacaaaacag agauaaagga     1980

gaccggacac ccuuugaaua cuucggagaa gaccaagagu caguagacug gaaacguuuu     2040

accaauuggg uagaaucaaa uccgcaaaug ccacaagcaa aaagaagaaa auuacuaaca     2100

aaaucacuac aaaaagguau auuagacaau uucucagcgc gaaaucuuuc cgauacgcga     2160

uauauagcua gagaacuuaa aacuuugauc gaacauuaca uucuuaauac uccagaaaga     2220

gagaaaacca aauaugaacg uuguguaguu auaagcggca aauuaaccgc acaaaugcga     2280

caacuauggg ggauauccaa agcacgagau guaagcgaca gacaccacgg cagcgaugca     2340

gcaguaaucg cauguaucac accaucaaug guaagacgaa ucacaaggua cuuacagcua     2400

caggaacgac gugcaaugcc aagcgcacca ggaaaaggag augaacaccc agcaguaguu     2460

gcaagagcaa uuccacacgc accaguacca uggccaacau ucgaacacga uuuagaagcu     2520

gcacucgaaa aaguacucgu aucacgaccg caauccagaa aauggagcgg accaauacac     2580

gcagauacca uauauggaga acacaaagau caagaaacaa auaccgucgc uccaacucaa     2640

cgaaucaaau uaacacaacu aaccaaaagu aacuauaaaa auaucgucgg auacgacgaa     2700

gaacgaaaca aagacuucau agaccagaua gaagaacgcc uagaaugcca uaauuuuaac     2760

ggaaaacaag cauucggaac aagccaaucc ccuauauauc gcauuaagaa aaauggagaa     2820

cuaggcccag aagugaagaa agucacuaua aaacucccga ucaaaaacuc ugucuuuagg     2880

auaagacgug gauaugucaa aaacgcuaaa aauucaacga uauacauaaa uaucuaucaa     2940

aauacuaaaa caggaaaauu cgaaaugcau ccaguauacg caaaagacuu aacaucuagu     3000

gcaaacgcaa gcaaaucauu ugaauacuca ccagaccuac agcuaguuga uacaauaaga     3060

cgaggagacu acauagaaau aaccagaaga aacggagaag uaauaucagg auacuaugca     3120

acacauaaca uaucaacauc cguucuagaa uuacaaccac augacacuac agacaguagg     3180

aauaaccgca caccagcaau caaaacauca cuaaacaucg aacgauuuca uauugauaua     3240

uuagggagaa aacgcagagu gguaaggaga gcgaaaaaug gccuagcuga ugacagggac     3300

aaauga                                                                3306


<210>  5073
<211>  3309
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5073
auggguuaua ggaucggacu agacauaggc auaaccuccg uuggauaugc aauacuaaaa       60

acagacgaaa aaggaucacc guacaaaauc gaacauuuaa cuucuguaau cuucccaaua      120

gcagaaaauc caaaagacgg aaagagccuu gcaaugccaa gaagagagca aagaggcgca      180

agacgcagaa acagaaggac aaaauucaga aaacaccgaa cgaaaauguu auucguaagg      240

ucggaauuac uaacgauaaa acaaauagaa gcaauuuaca gcgaaaaaca aaaacuauca      300

uccauauaug aguuacgaac aaaagcacua aacgaaagac ucacuaauga agaauuauuu      360

cgaauacuau auuucuuuuc aggucaccgg ggauuuaaau cuaauaggaa aagugaacuc      420

acagaaaaag acaugggucc aguauuaaca gcaauaaaag auauagaaga aaaguuagca      480

gauggaacau acagaacuuu gggagaauac auggaauuag acgaaaaaua ccaagaacac      540

aaaagaaaca aagacgguau agaucgguau cuaggaacug ccaaaagaag ccuaauugaa      600

gaugaaauua aacaaauuau aauagcacaa caacaauucg gaaacacgaa acuaacaagu      660

ggauuccaag uagcuuuuau aggagacgaa gaacaaaccg gaauauucaa cgaucagcgg      720

gaauuugaag augggccugg uaaacaaagu ccguaugccg gaaaucaaau agagaaaaug      780

auaggagccu gcacauuuga agaaaaugag aaaagagcuc cgaaagcauc auauaccuuc      840

caauacuuug acuuccucug caaacuaaau aaccuaaaau acagagaaac aaucggaggg      900

gaauaccgau uccuaagucu ugaacaaaag aacguauuga aagaaaaggu guugaccaca      960

cagaaaauca ccuuuucagg ugucaagaaa uuacucgacu uaccagaaca cgcacaauuu     1020

aaccuaguaa acuacggaac gaaaagcaca gucaaagaaa cagaaacuaa aucaguauuu     1080

uaugcuauga uaucuuacca caaauuaaag aaagcaauac cggcaccaau cuuuaguaac     1140

uuaagcacag cagaauugga cgaaauaggc agaauacuua cauuauacuc aucagacaaa     1200

ggucggaaaa augaguucua cagcagauua gcuauaccag aagaaguagu agaagcauua     1260

uuaccaauaa auuuuaguaa auucggaaac cuaagccuga aagccaugag aaaaauuaua     1320

cccuaucugg agaugggucu aguuuacucu gaagcugcaa cacaagcagg auacgacuuc     1380

agacauaaga aaauagacca ggcuuauaua cacgaaaacg uaacaaaucc ggucguaaaa     1440

cgggccgccu cacaaaccau aaaaauagua aauaauauaa uuaggaaaua cggagaaccg     1500

gacggaauca acauagaauu agcaagagaa cuaagcaaaa auuucaguga acgcaagaaa     1560

acgguuaaau aucaagaaga aaaucgugca agaaacgaaa gaauugcaga caaauuaaga     1620

gaaugcgaau ugccugucaa cggagccaau aucacacguc uaaaauuauu ugaagaacaa     1680

ggagaauuug auccauacac cggauuaaaa auacccuuug aucgagccuu uucugaagaa     1740

uacgaaguag aucacauaau accuuauucu aaaacauuag augacaguua cggaaauaaa     1800

auacuuguuu cugcaaaagc gaaucaagaa aaagggaaca gaauccccau ggaauacuua     1860

caaggaaaac cugaaagaau aaagaaacua gaaaguauua ccgcgacaau caaaaacccu     1920

aagaaacgag aaaaacuacu aaaacaacaa uugaccaagg aggauguaga cggcuggaaa     1980

acuaggaauu uaaaugacac aagguauaua agcagcuuac uacaugacua uuuuaaccaa     2040

aauauaucau ucgcaaaacu agaaucagaa aagaagaaaa gaguaauagc cguuaaugga     2100

gcaauaacau cuaaacucag agcaagaugg gguuuuaaca aaauacgaga aaauggagac     2160

gaacaucaug cacuugaugc guugauagua gcguguguaa cagacaaaua cauaaaagag     2220

auaacgagcu uuucaaaacg aaaagaaguu agauucaacg aaaacuugug gaaaagggag     2280

aaaacaccac aagaaauaga acaagaagua guacacuuua agaaagauua cgaucgaaua     2340

uucaaucaag ccuucccauu acccuggaca aacuuccgcg aagaaguauc augcagaaug     2400

ucugaaaacc cagcagaacu aaugaaaaau cauacauggg aaacauauac agacgaugaa     2460

auagcagcuc uacgacccau uuucauagua agaaugccaa auaugaaaac aucagguccc     2520

gcucaucaag aaaccauaag aagugguaaa cuagaagaaa aaggauuaaa cauaucgagg     2580

guaagcauag aaaaacuaaa auuagauaaa gaaggaacga uuaaaucagg uaacgcacaa     2640

uuuuacaaag auucaggaaa ugguuggcaa cuguuacacg acaaacucaa aguggccuug     2700

gaaaaacaug acggagaugg uaguaaagca uucccggacg gaguguucaa auaucaauac     2760

uuagauaaag aacacacugu aagaaaaguu agauuaguag caaaaucaac guuacaaaca     2820

ucacugaaug aaaagaaauc aguagcagac aacggaucaa ugauaagaau agaugucuuc     2880

aggaaugagc aagggaaaua ccaauuuguu cccauauacg caaaagaucg aguugcaaag     2940

aaacuaccua accaagcugu cgcuguagga aaaauagcaa aagaauggcc acuaauagaa     3000

gaaucagauu uuaaauuuuc acuuuauagc aaugaucuaa ucaggguaaa acacaagaaa     3060

ggcguaaaac acguaucuaa agacggaaac aaagaagaaa aauccaaaac agacuuuuuc     3120

ggauacuaua cgggagccga uaucgcuaca gcaaguauaa gcggcauauc uccuaacaau     3180

uucuucgaau uuagaucaau gggauuagca acauuaauca gcaucgaaaa auacaaagua     3240

gauuacuucg ggaauuacca ccaaguaaau gaaaaagugc gccaaaaauu cccuaaaaac     3300

aggauguga                                                             3309


<210>  5074
<211>  3312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5074
augaaauaug acauaggcau agacaucggu gucgcaagug ugggagaagc aguaauagau       60

caggagggaa acauccuuga agcuugcagu aauuuauucg augaggcaga ugccgcauca      120

aauguagaca gaaggaacuu uagagagggc agaagaaaua aaagaagaga aagaacaaga      180

guaaaugauu uuaaaaaacu auggacaaaa uuuggauuug aaauaccgaa aaacguaaug      240

aacgauacaa uauuacuaag gaacaaagga auaaagugug aauuagaucu aacggaacuc      300

uacagcguau uauuauacau guuaaaacau cgaggaauca gcuaccucga agacgccaua      360

gacgaagcca aagggucuaa cuacgccaaa ggcauagccu uaaaccagaa agaacucaaa      420

gaaaaauuac cgugcgaaau ucaacuugaa agacuaaaaa uauauggaag uuacagggga      480

gauuguauag uaaaaaaaga agaugaagac gaauaucacu caaacguguu uacuauaagu      540

gcauacaaaa aagaauuaga gauacuauuu agaaaucaaa aacuaccaga agaauuuaua      600

caaggauaua ugaaaauuuu cgaacgaaag agggaauacu auaucggacc aggaaacgaa      660

aaaagucgga cagacuaugg aguauacacc accaauaaag augaagaagg acacuacaua      720

acugacaaaa acauauucga aaaauuaaua ggaaaaugua guguuuauaa agacgaguuu      780

agagcagcag gcgccuccua cacagcacaa gaauuuaacg uauugaauga ccucaauaac      840

uuaacaguaa acaacaggaa acugacagaa aacgagaaaa aaaaaauagu ggaaaaaauc      900

aaaagcucaa augcagugaa caugagguca auaauaaaaa aaguuuuagg agaagauauc      960

gaacaguuug guggugcacg aaaagauaaa gcagacaaag aaauauauca cacauuugaa     1020

guauauagaa aaaugaaaaa agaguucgaa aaaaacggca ucgauaucgc agacuuuucg     1080

cgcgaaaaau uagacgaaau uggaaauaua cuaacacuaa auaccgaaac agaaggaauu     1140

aaaggcgcac ugcacgaagc uaaaaauuca uucacuuacu cagaaauaga gacacuuaua     1200

gacauacgca aaaaaaacuc aucacuuuuu ucuaaguggc aaagccuauc acuaaaaauc     1260

augaacgaac uaauaccuca aauguacgca cagcccaaag aacaaaugca acuauuaaca     1320

gaaaugggag uuuucaaauu caaguccgua aaauuuaaaa acaguaaaua uauacccgua     1380

gaagaaauaa ucaaggacau uuacaauccu guugucguua gaucaguaag aacaacaauc     1440

aaaaugauaa acgaaacaau acgaaaauac ggauacccug acaauaucau cauagaaaug     1500

ccacgcgaua aaaacaguac cgaagaaaaa gaacgaauaa aaaaagaaca aucaaaaaau     1560

gaaaaagaau augaaggaau acuaaaaaga auaaaggaug aguaugacau agaaauuaau     1620

agagaugacu auagaaaaca agaaaaacua gcuuuaaaac uaaaauuaug gaaugaacaa     1680

gagggaagau gcccauacuc ugguaaaaug aucagcauau acggauuauu agaagaccca     1740

caguuauucg aaauagauca cauaauccca aaaucgauaa guuuugauga cucaaggaau     1800

aauaaaguuu uaguauaucg aagugaaaau caaaaaaaag gaaauaauac accuuacaug     1860

uauuuaagca aaggcgauaa aaacuggaac uacgaucaau auaaaaguua ugugcuggga     1920

uuaaagaaca aaggaaacau aucagacaag aaggucaaca acaugcucuu uauggaagac     1980

auuaacaaaa uugauguguu aaagggguuc auaacacgua aucuuaacga cacaagauau     2040

gcuucacgag uaguacuaaa uaugcuacaa agcuucuuca augaaaaaga ugguuguccc     2100

accaaaguca aggugauaag aggaucguuu acacaccaaa ugagaguaaa uuugaggauc     2160

aauaaagaca gagacgaaag uuauagucac caugcaguag acgcaauguu aauaguauac     2220

agucaaaggg gauacgaagc auauagaaaa auacaagaag ccuguguaga cuuugaaacg     2280

ggagaaauaa uagaugaaga aagguggcaa caaugcauau ggggcaaaga uuacgaccaa     2340

guaauguacg aagcaaagug gagcaaaauc cgagaaaaaa uaauagaagc agaaaaaaau     2400

guuaaguauc accaucgagu cgacaaaaaa gugaacaggg gauuaugcaa ucaaacaaua     2460

uaugguacca gaauaaaaga cgggaaaaua uacaaaauau cauccuacaa uauauacgac     2520

aauaaagaau gugccagucu acucaaaaug aucaaaggag gaaaagaaag caaaauccuc     2580

auguacaacu acgauucaaa aacguacaga gacaugauca aaauaauaga cgaauauuca     2640

gacgagaaaa auccauuugu agcauauaug aaagaaacag gagacuacuu ucgaaaauac     2700

gccaaaaaac acaacgggcc aaagauagaa aaagucaaau acuacaaaga agaggcagga     2760

ucauguauag acauaucaca caaauacgga uaugaaaaag ggucgaaaaa agucauacug     2820

ggaucauuga accccuauag aacggauguc uucuuuaaug aaagauuaaa gaaauauuau     2880

cuaauuggga uaaaauauaa ccacaucaaa ugcgaaggga auaacuacgu aauagaugaa     2940

gacgcauaua acggauuacu uagagacgca ggagucauaa gucaugaaaa uacaaucaau     3000

gauuuggaaa aggucggagu aaaauacuua uucagucucu auaaaaacga uaucauaaaa     3060

uacgaaaaaa auggcgaaau guuuguugaa agauucuuau ccagaacuau ggaauaugca     3120

aauaauuaca ucgaaacaaa accaauucac aaaccauuuu uccaaaagaa agacaaaaaa     3180

ggaaacauaa aaaauagcca aaaccucgua ggauugagca aaacaaaaua cauaggaaaa     3240

cucguaacag acgcauuagg caauacuuac augguagaaa aagaaaaguu uucacuaauc     3300

gucgauauau ga                                                         3312


<210>  5075
<211>  3315
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5075
augaaacuua uauuagccuu agauaucggc auagcaucug uaggaugggc aguauuagac       60

aagaaaagug aaacaguacu cgaagcuggc ucaaacaucu uuccagaagc aagugcagcc      120

ggaaaucaag uaagacgaga aaugagacaa gcaaggcgga uaaaacgaag acaaaggacc      180

agauuagaug acuucaacaa acuguggcgg aaaaauaacc uaaguauacc ccaauauaaa      240

agcaccgaaa uaguuaaucu aaaaguaaaa gccuuaagag aagaaauauc acucgacgaa      300

cuuuaccuaa uauuauacuc uuacuuaaaa cacagaggca uuucuuauuu agaagaugca      360

caagacgacu cccuuucauc uucaaacgcc uauacaaaug gacuacgaga aaacgcaaaa      420

gcucuagaaa aacacuaccc augugaaaua caaagagaca gacuacaaac gauuggaaaa      480

uaucgugggc aaacgcaaau aaccgacgac aauaaugaga aaauagaucu aucuaaugua      540

uuuacuauug gagcauacag gaaagaaauc aagaaaguac uggaaacaca aaaacucuau      600

cauaaugaau uaacggacga acuaugugac gauuacauau uaauauuuaa ccgaaaaaga      660

aaauauuaug aaggacccgg caacgaaaaa uccaggaccg acuauggacg auuuacaacu      720

cgccuugauc aaaaugggaa cuauauagac gaagaaaaua uuuucgaaaa acuuauagga      780

aaaugcuccg uuuacccuga cgaacuucgu ggagcagcag caucauacac ugcucaagaa      840

uuuaaccucc uaaaugacuu aaacaaccuc acgauaaacg gaaggaaauu ggaagaaagc      900

gaaaaacacg ccauuguuca aagaauaaaa acaagcaaca cgguuaacau gagaaaaaua      960

auagcagauu gcaugggcga aacaauagaa aacuucagug gagcgcgaau agacaaaagc     1020

ggaaaagaac aauuccacaa auucgaaaca uauaacaaaa ugagaaaauc acuaucagaa     1080

auaggaacgg auauugacga auuuucucga gaugaacuag acaaaauagg auacauuaug     1140

acaaucaaca cagauaaaga agcucuaaug gaagcauuca acgaaagcgg guuaaaccua     1200

gacgacucag ucaaagaaug ucugauauca uuccgaaaag ccaauucauc gagcuuuagc     1260

aaauggcaau cauucagcuu aaaaauaaug aaugaguuaa uuccagaaau guacauacaa     1320

ccaaaagaac aaaugaccuu acuaaccgaa augggaguag caaaaggcuc aucaggugac     1380

uucgccggac ucaauuacau accaguagau guaauaagug acgaaauaua uaacccaguu     1440

gucagaagau cuguccgaau aucauuuaaa auucuaaaug caguucuaaa gaaauauaaa     1500

guuuuagaca caauaguaau ugaaaugccc agagacagaa acucugauga agaaagaaaa     1560

agaauaacag acagucaaaa acuaaacgaa aaagaaaugg aguucauaga aggaaaacua     1620

gcccagaucu auaacaucaa acuuucauca ucagauuuca guucacaaaa acaacucagc     1680

cuaaaacuaa aacuauggaa cgaacaagac ggaauaugcc uauacuccgg ccgcacuaua     1740

gaccccgaag acaucaucaa aaacccggau cuuuuugaaa uagaccauau aauaccaaga     1800

ucaaucucau uugaugauuc aaggucuaau aaaguccuag uauacaggag ugaaaaccaa     1860

aagaaaggca aucaaacccc guacuucuac auaacucacu cagauggagc auggaccuau     1920

gaacaauuua aagcaaaugu aaugaauuua agcaaaaaga aagaauaugc aauaagcaga     1980

aagaaaguuc aaaaccuacu cuacucagaa gauauaacga aaagagaugu auuaaaaggu     2040

uucauaaauc ggaauauaaa cgauacuggu uacgcaagcc gaguaguacu caauaccgua     2100

caaaacuucu ucaaagcaaa agaagcagau acaaaaguaa aagucauaaa agggucuuau     2160

acacaucaaa ugagaucaaa caugaaacuu gacaaaaaca gagaugaaag cuauucgcau     2220

caugcugucg augcgauguu gaugggauuc uccgaaaugg gguaugaagc auacagaaga     2280

uuacaaggag aguucauaga cuuugaaacc ggugaaaucc uuaaaaauga uauguggaau     2340

gagaaaauga gugacgacgu uuacguagac uaccuauacg gaagaaaaug gucagccaua     2400

agaaacgaaa ucgugaaagc ugaaaaagau guaaaauuuu ggcauuuuau uguuagaaaa     2460

ucaaaccaag gacuaugcaa ucaaacaaua agaggaacga gagaauacga cggaaagaaa     2520

uacaaaauaa auaaauucga cauaagaacc aaagaaggcg ucaaacuauu uaagaaacua     2580

gcuuucucgg aaaaagaauc cgauagagaa aaacuauuag uauaucuaaa cgacagacag     2640

acauucaaca aucuaguaaa aauauuugaa gacuacaaag augcgccaaa cccauucgug     2700

caauaugaaa aagaaacagg cgacguagua aggaaauaca guaaaagaca caauggaccc     2760

agaauagaca aacuaaaaua caaagauggc gaaguagggg cguguauuga uauaucacac     2820

aaauacggac ucgaaaaaga cucaagaaaa gucauuuugg aaaguuuagu ccccuauaga     2880

auggacgucu acuacaaaga aagugauaaa auguacuauc uugugggagu aaaacaaagu     2940

gacauaaaau cacaaaaugg ugccaacgua auagacgaag aucuauacac ccgcauauua     3000

auaaaugaga aaaugauaga accaggucaa ucuagagacg accucgaaca auuaggauau     3060

gaauuuagau ucaguuuuua caaaaacgau auaauagaau acgaaaaaga aggaaaagua     3120

uucguagaaa gauucguguc aagaacaaug ccaaaaguac gcaauuacau agaaacaaaa     3180

ccaaucaaca aagccaauuu cgaaaaacaa aauccaguag guuuaggaaa aacuaaaucu     3240

aucaagaaau acagaacaga caucuugggc aacagauauu uaugcgaaaa agaaaaauuu     3300

acgaaauuuu gcuga                                                      3315


<210>  5076
<211>  3315
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5076
augaaccacu auguacuagg acuagacauc ggcaucacau caguuggcua uggcgucauc       60

gauuuggaua auuaucaauu uguggauuac ggaguuagau uauuuaagga aggaaccgca      120

gaagauaaug uaaauaggag aacuaaaaga ggcucuagga gacuuaaaag aagacgucaa      180

acuagacuag acgauaugaa agaacuacua caaaaagaaa aaaucauguc agaaacauau      240

cauagcagcu augauccaua ugacaugaga auaaagggac uaucccaaaa auuaucaaac      300

gacgaacuua ccugugccau acuacacauc accaagugua ggggaacaac ucuagaagca      360

cuagacucag ccgaugaaga cuccgaagga acaaaaucaa uacugucuaa aaacgaccaa      420

gaccucaaaa aauaccacua cguaugucuc guccaaaaag aaagauuaaa acaauccgga      480

aaaauuagag guaccgaaaa uaacuucaaa accgacgauu acauaaaaga guuagacgaa      540

auauuaucac accaagaccu uuccgaagac uuaugugaaa aaauacguac cauaguaagc      600

aggagaagac aauacuacga aggccccgga ucagaaaaaa gcccuacccc auauggaaga      660

uggauagccc aaggaguagc cccaaucgac uugauagaaa aaaugagagg aaaaugcuca      720

guuuuuccug aucaacuacg ugcagcuaaa aacucauaca cggcagaauu auucaaucuu      780

cuaaaugacc uaaauaacuu ggaaugcgaa ggagaaaaau uaacgacauc acagaaacaa      840

gaugucauag acuucguaaa cgaaaaaggg ggcaucacuc ccaaacaacu ugcaaaauug      900

cucaacguug acuuaaacca gauaaaagga uucaggauag acaaaaauga uaaaccauua      960

cuaacagagu uuaaagguua uaaagcuuuc aagaaaauau uugauacuaa uaacugugau     1020

cuauauaaaa aagaucucuc aauucuugau ggcuuagcag aaauaguaac ugguaaaaaa     1080

gggaucgaag aaagaaaaca agcaauccaa caagcauacc cagaaaucga ugaagccaua     1140

gucgaacaau gcgcuguaau gaaaggaaug acacaauauc auucucuauc ccuaaaagcc     1200

augcaucuua uuaaccaaga aaugcuacaa ucaccaagaa aucaaaugca aguccuccau     1260

gaaucacacg uauucgacaa aaacagagua agucacaaag gacaaaaaua cauagaggca     1320

gaugaugaag caauuuuauc accuguagua aaacgagcac agagggaaac auuuaaagua     1380

guaaaugcac uaagagaaaa auauggugag uuugaaucaa uaguaguaga aacaaccaga     1440

gauagaaacu cacaagaaca gaaaaagcga cucaaugacu cacaaaaaua cuacgaacaa     1500

gaaaacagaa agguagacga acuccuaagu caugaaggcu acgacccuag caagauaaau     1560

gggaaaacaa aacuaaaagu aagacuauac uuacaacaag aaggaaaaac agcauauaca     1620

aaugcaccca uagaucuuaa uuuacugaua aaagacgcaa cagcauauga ggucgaccac     1680

auuauaccuc uaucaauaag uuuggaugau aguaucaaca auaaagcauu aauaaauaga     1740

acggaaaacc agaauaaauc aaacaugaca ccaaucuaug ccauccuacg aggaaaacuc     1800

aauauaucuc uagauacaua uaagaccgua guuaaaaaca acagaaauua cacaggcaaa     1860

aaaaggaguu aucuauuaua cgauaaagau auaaccaagu ucaguaacau gcaagaauuc     1920

auagcucgga auuuagucga cacauccuau gccugucgag uaguaauguc cacauuauca     1980

gacuauuuua aagacaauga aauaucaaca cauguacaua ccguuagagg ccgcguuacc     2040

aacauguucc gaaacagaau uaauaugcag aaagaaagag aaaaagauua cuuacaccac     2100

gcaguagacg cauuaaucgu ugcuagcaua aaaaaauuaa acuuauuaaa caccuauuua     2160

uuaaaguaca auuucaauga cuuauauaac gaagaaaccg gcgaagucuu cgcaguagga     2220

gacgaucaac aaguccugga cccaaaauac auaagauuca ucacucaacu gaaaacaaua     2280

uacgacgaau caaauaugua cuacaaugga uuaauagaac guaauguuau gcaauuccca     2340

ccaauaaaaa ucucgcacaa aauugauacg aaaccaaaca gacaaauaag cgaugaaacu     2400

auuuauucca cccgaaccgu aggcaacgaa gaauacguag uaaaaaaaua cucagacaua     2460

uacgacccca aauucaccca acuaaccgac gauauaauaa augaucggcc auuagacaga     2520

uggcuaaugu aucgaaacga caaccaaaca uuuaaaauaa uacagucgau cauauuagac     2580

cauuuccaca uauucaaaga cgacgaaaaa cacuauaguu guacaaaaaa aaaaggccaa     2640

auacaguaug cccuaaaagg gaagaauccc uuauaccuau acaaacaaga acauggaaaa     2700

guacguaaau acgcaaaaaa aggaaaugga ccagaaauca caaugaugaa auauuucgau     2760

ggcaaacucg gaaacaacau agacauauca aaccauuauc auguccacaa caaaaaaguc     2820

guacuacucc aaaucagccc auacagaaca gauuucuacg uaucaccaga aggaaaauac     2880

aaaaugcuaa caguaagaua ugcuaacguc uuuuauaaag agucaaaaaa gaaauacgua     2940

auagaugaaa aaugguacgc aagcgagaaa aaacgcaaaa acauaaccga cgacgauauc     3000

uucguaugua gccuacacca cgaugaguua auaggcauac aaaaaaaaga cggagcaaaa     3060

uauguuuacg accuuaguac ugaggacgga gguaaaccaa gauuauacca cggugaaaug     3120

gaaauacuaa aguuuacagc cacaaauaau gauucuaaaa acauuaucga agucaaacca     3180

auauacacau auugcaagaa acaacuaaug auaagcauag guccaauuac aaaacuugca     3240

aaauacgcaa cagauguacu aggaaaugua uaugaaguaa aaaaaaacau uauagaccac     3300

cuuucacacu uauga                                                      3315


<210>  5077
<211>  3318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5077
augggcauac gaauuggacu agauauugga auagcauccg uaggaugggc aguaguagac       60

gacgauuaua acauaauaga aucaggagca aacauauuca acgaagcaaa agcagcaucg      120

aacguagacc ggagaaccuu cagacaaacg aaacgauuau uaagaagaca gaaaacacga      180

uuaagagauu uugacauauu auggauaaac gcuggauuag acauccccaa agaaaaagua      240

aacaaacaau uacaauuuag aguucaaggc augacuaugg aacugagcga aaaugaacua      300

uaugcagcac uuaaaaauau gcuaaaacac agaggcauaa guuacuugga agacgcucuc      360

gaugaagaac aaaguggaaa aagcacauac gaaaaaggcu uacaaguuaa ccaaaaagcc      420

uuaaaaugug auaaaaugcc cugcgaaaua caauacgaac gucuuaugaa auacggauca      480

uauagaggcc auaucgaagu aaaagacgaa ucaggagaag aaaugauccu aucgaauauc      540

uuuacauuag acgcauacug caaagaagcc aagaaaauau uagaauguca aaaacaauau      600

cacuccuuca uuacagaggc uuuugaagau gauuaucuag cauuguugag aagaaaaaga      660

aaauacuacg aaggccccgg aaaugaacaa ucaaggacag acuacggcaa auacacgacu      720

aaacucaacc cagaaacagg cgaauacguc acugaagaaa acauauuuga aaaacuaauu      780

ggaaaaugcu cggucuaccc aaaagaaaug agagcaagcg cagccucaua uacagcucaa      840

auauauaaua ugcuuaauga uuuaaauaac uuaaccauaa auggcagaaa acuagaauau      900

gaagagaaga aaaguauagu agaagaagua caaaaagcaa gcagugucaa uaugcuaaaa      960

auaaucaaga aaguaauaaa ugaagauaua acaacaauag aaggagcacg uaaagacaaa     1020

aacgauaaag aaauauucca uaaaauggaa accuacaaca aaaugcguca uuacuuugag     1080

aaaaauggac uagaauuuaa gaaaauauca acagacgacu uagaugaaau agguaauaua     1140

cucacacuua auacagaaaa agaagcaaua uuagaggcuu uuaauaaauc ccaacucgaa     1200

uuaucacaag aagaaaaaga agcguuuaua gaaaucagga agaaaaacgg uagccuuuuc     1260

aacaaauggc acaguuucuc ucuaaaaaua augaaagaac uaauaccaga auuauaucua     1320

caaaguaaaa accaaaugca acuacuuaca gacaugggag uuuucaagaa gaaaacagaa     1380

auauuccaaa auuacgauaa aauacccacg gauguaauca uagaagacau auacaaccca     1440

guaguaaaac guagcauaag aauuacagug aaaauaguga acgcacucau caagaaauac     1500

ggauauccga gagaagucgu cauagaaaug ccaagagaua gaaacagcga ugaagagaag     1560

aaacgcauuc aagacgaaca aaagaaaaac gauacagaac uaaagaaaau aauagaaaaa     1620

gucgaaucag aauacggaau aaaaauaaca gacacccacu uuagaaacca uaaaaaccua     1680

agaaugaaac ugagauuaug gcacgaacaa gacggccgcu gccuauauag uggcaaaguc     1740

auagauaucu cagaacuacu aaacgauugc aguaaauucg aaauagauca cguaauucca     1800

aaaucuauau cauucgacga uucaagaagu aauaaaguau uagucuacag cacagaaaac     1860

caaaagaaaa caaaccaaac accauaccaa uauuuggagg gaaacggaaa agaaugguca     1920

uucgaugaau acaugaacca agugauagag uuaaaagaca gaaaacuaau auccaaagcg     1980

aaaaugaaca aauuauuauu cagagaagau auaacaaaaa uagaaguucu aaaaggauuc     2040

auagcucgua acauuaacga uacucguuau gcaaguagag uaguacuaaa uucauuccag     2100

aaauauuuug agaguaaaca aucagacaca aaagucaaag uaauaagagg auccuucaca     2160

caccaaauga ggaagaaauu gcaauugagc aaaaauaggg augaauccua cgcacaccac     2220

gcaguugacg caauguugau augcuacagu caaaugggau acgaggcuua cagacaauua     2280

caagaagauu ugauugaucc ggacucagag aaaauucgcg accucgggcu auggaaagaa     2340

acuauaacug aagcgaaaua ugaugaagua cuauacaccg caaaauggcu cgaaaaccga     2400

ggaaaaauac aagaagcuga gaagaaagua aaauucuggc auaaaguaga cagcaaagca     2460

aacagggcau uaugcaacca gacuauaaga ggaacuagag uaauagacgg aagcauacaa     2520

aagauaaaua aaauuaauau auacucagug gauggauaua aaacguuuaa aaacaugauc     2580

caaaaaggaa aagaaaaaga uuuccuaaug uacagaaaug auaggagauc cugggacgac     2640

uuaaugaaag ucuaccgaga auacucagac gccaaaaacg cguucgcagc auaccaagaa     2700

gaaacgggag auuauguuag aaaauaugca aagaaacaca auggaucaaa aauaacuaac     2760

cugaaauacu gugccaaaga acuagggagu ugcauagaca ucagccacaa auauggucug     2820

gaaaaagacu cuagaaaagu aauuuuggaa agucuaagcc cuuauaggag cgacguuuac     2880

uauaacgcag gcuuaaaaca auacuacaua guugggauca aaaauaguga ucucugcuuu     2940

uuaaaaggag aguacuuuau agacucaaaa agauauuuac aaauacucag ggaagagggc     3000

guccugaccg aacaacacac cuuaaaugau uuagcagcua uaggcacaga guuccaauuc     3060

agcuuguacg aaaacgacua ccuagaauau gagaaaaaug gagaaauaaa aacagaaagg     3120

uucuuaucga ggacuaaacc uaaacagaaa aacuauauag aaacaaaauc aauagaugcg     3180

ccuaaaauua aaaacaagaa acaaaaucuu gugggacuug cgaaaagcaa aaguauaaag     3240

aaaauaagag uagauauauu agguaaucga uauuaugcaa guaaagaaaa auuuagcuua     3300

augguagaca aauucuga                                                   3318


<210>  5078
<211>  3318
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5078
auggauaugg cuuggagacu aggacuagau cuaggcacaa auucuuuggg uugggcagca       60

uuaagccuag augcagcagg agcaccagac gcuauauuag cagcuggauc aagaauauuu      120

ggggacgguc gugauccuca gagugggaca ucacuugcgg uagacaggag agccgcuaga      180

gcggcaagaa gacgaagaga cagauucaaa cagcgacaac gcgcacuuuu aaaacaccug      240

gaagcagaug gcuuauuucc cgcugauccc gagguacaac aagcucuagc agcuuuagau      300

ccguaugcuu uaagagccag agcauuagau gaagcacuau cacuucauga aauaggacga      360

gccuuauuuc auuuaaacca gagaagaggg uuucaaucga auagaaaagc agauagggga      420

aaagaugaag acgcgggaaa gauagcgaua ggaguagaua gauuaaaaga cgcuauugcg      480

gcugcuggag cacgaaccuu uggugaauuu uugcgucaaa gaagggcugg agcaaccgga      540

gagaaccaaa uuccuagugu aagaacaaga cugagggcgg agacgggaga aggagcgaaa      600

gggaguggau augacuucua cccaucaaga gcguuacuaa aagacgaauu ugacgcgauc      660

uggcaugccc aagcagaaca ccaucccaaa guauuaacug augaagccua ucacagacua      720

cacgaaauag uauuucgcca aagaccacuc agagcaccua aagugggggc augcacauua      780

guuccugggg aggcuagauu gccaaaagca cauccuuuau uucaaaggag aagacucuua      840

gaggaauuaa augcacuaau gauaguaagg gcuggagcgg uggcggaaag guuaacucca      900

gaacaaagag accuacuauu acugaaacuu aaggauaaag gcaagguuac auuuaaguca      960

uuacgugaaa aaguuuuaaa acuugacggu gaugcuagau ucaacaaaga aagugaacau     1020

aggacagaac uuaaaggaga cgaaguagca gcagaaaugg guggaaaaac gagguuuggu     1080

ucacucuggc cccaucuguc aacagagcaa caguggacag uaauagaaag acaacaagcg     1140

cuagaaucgg acgcagacga agcggcguuu agaacauggc uaguggagac acauaaacuc     1200

acagcagaac aagcaagagc uguagccgga gcacgcuuac ccgccggaca cggaagguuc     1260

ggacucacgg cuaccgcugc acuuuuagca gcauuacggg auggccgcac accugaggga     1320

agagggccca augcgccagc uacugaagca ggaagaguag uuguguacuc ggaagccgca     1380

gcaauagcag gauaucacca uagcgaucau aggaguggug agguauucac agaugcaaag     1440

gguaggccug cauuaccuua cuauggagug ccacucgauc gacauauagu accuggaaca     1500

gcagauccag augagcccga cgaagccgca cguauaggga gauugacaaa cccgacugua     1560

cauauagcac uaaaccaauu acgucgaguu gucaauaggc uuauaagagu auauggacca     1620

ccagcagagg uagcauuaga auuagcgaga gaacucaagu uaagugaaga cgaaaagaaa     1680

gagagaaauc gagaaaauuc uaggaacaga cuugaugccg aaaagcgaag uucuaaauua     1740

gccgaauuag ggcagcgaga caauggugga aauagagcau uauuaaaacu uugggaagaa     1800

uuaaacccag aaaauauauu agaccgucga uguauauacu caggucgaca aaucaguauu     1860

ggaaugauuu ucucaggagc ugcugaaguc gaucauauac uuccuuuuga ugcaacauua     1920

gacgacucaa acgcuaauaa aauuuuaugu uuacgagaag ccaauagaga uaaaagaaaa     1980

cguaguccuu uugaagccug gggugggaca ccucaauggg aagagauagc ugagagggca     2040

agucguuuac caagaaauaa aagauggagg uuugaaccag acgcaaugga aagauuugca     2100

gaagaaggug guuuucuagc uagacauuug guagacacac aguauuuagg acgaauugcu     2160

caugacuauu uaagauguuu auauccagac aaaggcgaug gcagcucaca cgucugggua     2220

ucaccgggaa gacuaacaga aaugguacga agaaaacuug gauuaaaugg auuguuaggu     2280

gaucauaauc uaggagcaga acaaccuaag aacagaaaag aucacagaca ucacgccaua     2340

gaugcaguug uaacagcuau auuagauaga ucaauguuac aacguauuca acgagcuucu     2400

ggugaggggg cgucugacgc agaauugcug aggauaaucg ucccugagcc uuggacggga     2460

uuucgagaug acuuacaaag agccguggac aggauaguag ucgcacauag ggcagaucau     2520

gggacuguug cgaaagcagc aacacgaggu agagaucaaa cagcugcacg auugcacaac     2580

gauacagcau auggauuuac aggagaagca gaugcuaaag gaacaccuau uguaguacac     2640

agagcaccac uaggagcauu aaagaagccc gaacacauug aucaaguaag agauccauua     2700

uuaagagcag cacuucacga uuuuaccgcu ggccuaucag gaaaaccauu cgaggacaga     2760

auagcagcau uuccaagauu aggaccacua gacuaucgag guauaagacg cguuagagua     2820

guugaaccuc uacaaguuau accaauaaga gaugcagcug gcagagcuua uaaagguuau     2880

aaaggugauu ccaauuaucg auaugacgua ugggaauuac cugacggaaa gugggaacaa     2940

aggguuuugc aaauguauua cgcucaucaa ucaggagaac caccuaggcc ccacccugcu     3000

gcuagaaagg uccuuucacu ucauagagau gauguacuag ccauugaaag aggugaagga     3060

gguagagaau uaguacgugu uguaaaauuu ucaacaaaug aauuuacucu ggcaccguua     3120

aaugaaggug gggcucucaa aagcaggcac gccgacaagg gcgauccguu cagauauuua     3180

uauccaucuc caucaacuuu aaaagcgugg agagcaagag aaguaagggu cgaugagcuu     3240

ggacgaguau uagacccagg guuuccggca aggaaaagaa gaagaguuac aagaccgggg     3300

agagcagaug cugauuga                                                   3318


<210>  5079
<211>  3321
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5079
augcaauaca uuguggcuau agauauaggu guggcaucag uaggguaugc aguaauagac       60

aaaacaagug aaacggugau agaagcaggc ucaaacauau uuccagaagc aagugcaaca      120

gacaaucaag aaagaagggg uaugcgacaa gccagaagau ugaaacgccg agaaagaaca      180

agauuagacg auuucaccaa auuaugggaa uaccacgggc uugcaauacc acaagucaag      240

aaaaaugaca uuguaggacu aaaaguaaaa gcacucuacg aauccaucag uuuagaugaa      300

guauaccuaa uacucuauag uuacuuaaaa caccggggaa ucucauauuu agaagacgua      360

gaagauucau cgguaucagg aaguacagcu uaugcaaaug gucuaaaacu aaaugcaaaa      420

gaacuagaga aaaacuuccc augcgaaauc caaaaauaca gacuagaaaa cauaggaaaa      480

uauagaggac aaauacaaau agaagaugac caaggagaaa aauuagauuu aaguaaugua      540

uuuacaauug gagcuuacac aaaagaaaua caacaaauau uagacacaca aaagaaauac      600

caugcagaaa ucgaagacga guucugugaa aaauaccuaa aaauauucac acgaaaaaga      660

aaauauuaug aagguccugg uaaugaaaaa ucaagaaccg auuacgggaa auacacgacc      720

agacucaaag aaaauggaga auauauaacu gagaaaaaua uuuucgaaaa acuaaucgga      780

aaaugcucag uauacaaaga ugaacugcga gccgcuucag caucauacac ggcacaagag      840

uucaaccugc ucaaugaucu caacaaucua accguaaaug gaagaaaacu agaagaaaau      900

gaaaaacacc ucauagucga caaagugaaa acgaguaaua caaucaacau gagaaaaaua      960

auagcagacg caauggguga aaagauugaa gaguucuacg gagcaagaau ugacaaaagu     1020

gaaaaagaaa uauuccauaa auuugaagua uauaacaaaa ugagaaaagc acuagcagcu     1080

auccauguaa auauagaaga guucagcaga gacgacuuag acgaaauagg auacauacuu     1140

accauaaaca ccgauaaaga agcuaugaug gaagcauuug acaauucccg auuagaauua     1200

ccaggcgaaa ucaaaaacug ccuaauuagc cuacgaaaaa cuaauggcac ucuauacuca     1260

aaauggcacu cguuuagcuu aaaaauaaug aaugaauuaa uaccagaaau guacaggcaa     1320

ccaaaagaac aaaugacauu acuaacagaa augggaguaa ugaaagggca aacugaugag     1380

uucgagaaaa auaaauacau ccccguugac guagcgucag aagacaucuu caauccagua     1440

guaaggaggu caguacgcau uagcuuuaaa auacuaaacg cauuaaugaa gaaauaugga     1500

acuuuagacg aagucgugau agaaaugccc agagacagga auagugauga acagaagaaa     1560

ugggaaaaag aaacacagaa gaaaaacgaa aaagaacaag aauauauaga aaacaaacua     1620

gcuacagugu acgacauaag gcuuucgucc gcggacuuca gcucccaaaa acaacugaau     1680

uuaaaacuga aauuauggaa cgagcaagac ggaaaauguu uguacuccgg aaaaaucaua     1740

gaaccagaag auuuaauuaa acauagugaa cuauucgaaa uagaucauau uaucccuaga     1800

ucgauuucau ucgacgauag uagaucaaau aaaguuuuag uauauagaac agagaaccaa     1860

aacaaaggca aucaaacacc cuacuacuac uuaacacaca cuaacaguaa auggucauuc     1920

gagcaauaua aagcauuagu aauggaccug ucuaaaaaga aagaauaugg acuuagcaaa     1980

aagaaaguaa gaaacuuauu auacagcgag gacaucacca aagccgaugu acuaagagga     2040

uucauaaacc guaacauaaa cgauacaucc uaugcagcaa gaguaguucu uaauacagua     2100

caaggauauu uucgagcaaa agaaauggga accaaaguaa aaguuaucag aggaaguuac     2160

acacaccaaa ugagagugaa caugaaauua gauaaagacc gagaaaaauc auacgcucau     2220

caugcgguag acgcaaugcu aauagcauuu agucaauuag gauacgaagc cuacagaaaa     2280

uuacaaggag aauuuauaga uuuugagacc ggugaaauau uaaacagcga aaaauggaaa     2340

gaccaaaugu cagaugaggu auauaaacaa uaccuauaua gaaagaaaug gucuaauauu     2400

cgaaacgaaa uaacaagagc ugagaaaaac guaaaauacu gguauuaugu agaucauaaa     2460

agcaaucgcg gacucugcaa ucagaaaauu aggggaacca gaguguacga uggaaaaauc     2520

uacaaaauaa auaaacugga cauacgcaag aaagaaggag cagcacuauu uaagaaacua     2580

gcauuuucag aaaaagaaag ugaucgagaa aaacuauuag uauacaaaaa cgauaagaaa     2640

accuacgaag aucuaguuag aauaaugcaa gacuacucug acagcaaaaa uccguuugua     2700

caauacgaaa aagaaacugg cgauuaugua aggaaauaug ccaagaaaca uaacgggcca     2760

cgcauugaua aacuaaaaua cauagacggg gaaguaggau caugcauaga uauaucacac     2820

aaauacggcu acgaaaaagg cagcagaaaa guagugcuag aaagccuagu accuuauagg     2880

auggaugugu acuacaagaa agaagaacaa acauauuacu uagugggaau aaaacaaucg     2940

gaugugaaau gucaaaaagg agaauacguu auagaugaag acgcuuacgc cauaaaccua     3000

guuaaggaga aaaugauaca acaagggcaa aagagaagug auuuagaguc acuugguuuu     3060

gaguuccaac uaucauucua caaaaacgac auaauagaau augaaaaaga ugggaaaaua     3120

guaagagaaa gauuuuuaag cagaacaaug ccgaaaguca aaaauuacau ugaaacaaaa     3180

ccaauugaca gagcaaaaua cgaauggcau gauagaaagc uaguaggauu agggaaaacc     3240

aaaagaauua gaaaauaucg aauggacauc uuagggaacu acuacuugug caacaaagaa     3300

gaauuuagca aauacuguug a                                               3321


<210>  5080
<211>  3324
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5080
augggcuaca ccguaggauu agacauagga gucgcgucgg uaggaguagc aguacuagau       60

gagaaugaua acauaguaga agccguaagc aacauauuug augaagccga caccucgaau      120

aacaaaguac guaggacacu aagagaagga agacgcacaa aaagacguca aaaaacaaga      180

auagaagacu uuaaacaauu gugggaaaca aguggcuaca uaauaccuca caaacuucau      240

uuaaacauca uagaauuaag aaacaaagga cuaacagaac ucuuaucuuu agacgaacua      300

uauuguguac uauuaagcau guuaaaacac agaggaauau ccuaucuaga agacgccgac      360

gacggagaaa aaggcaacgc auacaaaaaa gggcuugcgu uuaaugaaaa gcaacugaaa      420

gaaaaaaugc caugcgaaau ccaauuagaa agaaugaaaa aauauggaaa guaccacggc      480

gaauuuauaa uagaaaucaa ugaugaaaaa gaauaccaau caaauguauu cacaacuaaa      540

gccuacaaaa aagaacuaga aaaaaucuuc gaaacacaac gaugcaacgg aaauaaaaua      600

aacacgaaau ucaucaaaaa auacauggag auauaugaaa ggaaaagaga auacuacaua      660

ggaccaggca acgaaaaauc ccgaacagac uaugguauuu acacaacgag gacagaugag      720

gaaggcaacu ucauagacga aaaaaauauu uucggaaagc uaauaggaaa auguucagua      780

uauccggaag aauaucgcgc cagcagcgca ucauacacag cccaagaauu caaccugcuu      840

aacgaucuaa auaaccuaaa aauaaauaac gaaaaauuaa cagaauuuca aaaaaaagaa      900

auagucgaaa uaauaaaaga cgcuucaucu gucaacauga ggaaaauaau caaaaaaguu      960

auagacgaag acauugaaca auauagcggc gcacguauag acaaaaaagg aaaagaaaua     1020

uaccacaccu ucgaaauaua cagaaaacua aaaaaagaac ucaagacuau aaacguagau     1080

auagacucau uuacgcgaga agaguuagau aaaacuaugg auauacuaac ccucaacaca     1140

gagagagaaa gcauaguaaa agcauucgau gaacaaaaau ucguauauga ggaaaauuua     1200

auaaagaaac ucauagaauu ucgcaaaaac aaucaaagac uauucucugg auggcacuca     1260

uucucauaca aagcgauguu acaauuaauu ccagucaugu auaaagaacc aaaagaacag     1320

augcaacuac uaaccgaaau gaacguauuu aaaagcaaaa aagaaaaaua ugugaacuac     1380

aaauacauac cagaaaacga aguagugaaa gaaaucuaca acccuguagu aguuaaaagc     1440

auaagaacca cugugaaaau acuaaacgca uuaaucaaaa aauauggcua uccagaaagc     1500

guaguaauug aaaugccucg ugacaaaaau ucagacgaug aaaaagaaaa aauagauaug     1560

aaccaaaaaa aaaaccaaga agaauaugaa aaaauacuaa acaaaaucua ugacgaaaaa     1620

gguauagaga ucacaaacaa agacuauaaa aagcagaaaa aacuaguacu aaaacucaaa     1680

cuauggaacg aacaggaggg ccuuuguuua uacucaggaa aaaaaauagc aauagaagau     1740

cuauuaaacc acccagaauu cuuugaaaua gaucacauca uacccaaauc aauaagccuu     1800

gaugacucaa gaucuaacaa aguacugguu uauaaaacgg aaaacucaau aaaagaaaac     1860

gacacacccu accauuaccu aacucggauu aacggcaaau ggggcuucga ugaauacaaa     1920

gcgaacguuc ucgaauuaag acgaaggggu aaaauagacg acaagaaagu caacaaucua     1980

cuguguaugg aagacauuac uaaaauagac gucguaaaag gauuuaucaa ccgaaaccua     2040

aacgacacgc gauacgcaag cagaguagua cuaaacgaga ugcaaagcuu cuuugaaagc     2100

aggaaauacu guaacacaaa gguaaagguc auaagaggga gccucacaua ucaaaugcgc     2160

caagaccuac accuaaaaaa aaacagagag gagucauacu cucaucaugc aguagacgcu     2220

augcuaauag cauucucaca aaaaggauac gaagcauacc gaaaaauuca gaaagauugc     2280

uaugacuuug aaacagguga aauauuagau aaagagaaau ggaauaaaua cauagaugac     2340

gaugaauuug acgacauauu auacaaagaa agaaugaaug agauccgaaa aaaaauuaua     2400

gaagcagaag aaaaaguaaa auauaauuac aaaauugaua aaaaguguaa cagaggacua     2460

uguaaucaaa caauauaugg aacacgagaa aaggauggaa aaauccacaa aauaagcuca     2520

uacaauauau augaugauaa agaaugcaac aguuuaaaaa aaaugaucaa cagcggaaag     2580

ggaagcgacu uauuaaugua caacaacgac cccaaaaccu aucgagacau guuaaaaaua     2640

uuagaaacau acucaagcga aaaaaacccc uucguagcau acaauaaaga aacaggagac     2700

uacuucagaa aauacaguaa aaaccacaac ggaccgaaag uagaaaaagu aaaauauuau     2760

ucagggcaaa uaaacucaug caucgacaua ucacauaaau acggacaugc gaaaaacucu     2820

aaaaaaguag uacucgucuc uuuaaaucca uacagaacag auguguacua ugauaacgau     2880

acuggaaaau auuaucuagu agggguuaaa uacaaccaca uaaaaugugu cggcaauaaa     2940

uacguaaucg acagugaaac cuacaaugaa cuacuaagaa aagaaggcgu uuuaaacucc     3000

gacgaaaauc ucgaagaccu aaacagcaaa aacaucacau acaaauucuc acuauacaaa     3060

aaugacauaa uacaauacga aaaaggaggg gaauauuaua ccgaaagauu ccuaucaaga     3120

auaaaagagc aaaaaaauuu aauagagacg aaaccaauaa acaaaccgaa uuuccaaaga     3180

aaaaacaaaa aaggagaaug ggagaauaca aggaaccaga uagcucuagc uaaaacaaaa     3240

uauguaggca aguuagucac agacguauua ggaaauuguu auauaguaaa cauggaaaaa     3300

uucagccuag uagucgacaa auga                                            3324


<210>  5081
<211>  3324
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5081
augggcuaua cacuagguuu agacaucggc aucgccucag uaggguuugg uuuaauugac       60

aaugucgaug gaaaaaucuu agaaagugga gucaggcuau uuucgagcgc agacccagau      120

aauaaucaaa caagaagauc caguagacaa gcuagaagac ugaacagaag gaaaguacau      180

aggcuagaaa gaguagcaca ucuucuagaa gaagcaggau uugaaaaagg agaagugcua      240

gcaccaaccc cguaucaucu acgaguaaaa ggacuuacag aaaaacuaag caaacccgaa      300

gucuaugcag cacuauacaa ucuaaugaaa cacagaggua ucuccuaucu cgaggaugua      360

gaauuagaug aggcugacuc cagcaguguc ucccaaaacg uugaacuacu aaaagaauau      420

uaucccugcg aaauacaauu agaaagauau gaagaauacg ggcaaauaag agguauaaua      480

gaaacauaca auaaagacgg agagaaagaa ucacugguaa auauauuccc aacaggagca      540

uacaagaaag aagcggaagc aauaauacua aaacaaggac aauuuuaccc agaauuagau      600

aaagucuuuu uagaaaaauu uacagaaaua cucacaguaa aaagaccaua uuacgucggg      660

ccugguaaca agaaaucaag aacagacuac gggaucuaua gagaaaaugg agagacauua      720

gaaaaucuau uugauauauu aauaggcaaa ugcacaauau auccucaaga agcaagggca      780

gcaaaauucu cauacacagc ccaacuauuc gacuucuuaa acgacaugaa caacauuucu      840

auagcuggag aaaaauuaac agaaguacag aaaagagaaa uauaucauca aauaaugucc      900

aguaaaacag uaaacaugau gaaaauaauc acaaaaguga cagguugccu aaaagaagaa      960

gucaccggau acagagucga cuccaaauac aaagcugagu uccacucguu cgcaagcuac     1020

agacaauuuc gcaauaaaau gcuugauuac ggcuuugaug uacaaacacu aaacguagaa     1080

auguucgaua aacucgcugc caaacuaacu uuaaauacag aauaucacca aauaauaaca     1140

caacuggaga gcuuaaaucu aggauuaucc aaagaacuaa uagagaaaau agccgauuuu     1200

cgaaagaaaa accgaucacu cuucucaaaa uggcacaguc uaucucuaaa agcuaugaau     1260

gaagucguag augaacuaug gucaacaagc aaaaaucaga ugcaaauuuu uagugaauua     1320

ggaggauuca aaucuaacau cgaaguuuuu aaaggacuua aauauauacc auuagaugca     1380

augguugaug aaaucuauaa cccaguagua agauguucua uuagggaaag cauuaaaauc     1440

auuaaugcac uacuuaagaa auacggugca cuagauaaag uaguaaucga aaugccaaga     1500

gacucuaaug aaaaagaaga aaaagaccgg aucacgaaac ugaauaaaga gaaugacaau     1560

aaaaagaaac aagccauaga gaaagcacgu aaugaguacg gauucggaga agaagcauac     1620

agacaacaga acgaccucaa uacaaaacua agauuauggu acgaacaaaa acaaagaugc     1680

uuguacucag gcgaaaucau aaaaauauuc gauuuaauua aaaauccaca ucuauuugac     1740

auagaucaua uuauccccaa gaguauuucc uacgacgaua gucuaucaaa caaaguauua     1800

ugcuacgcaa ccgaaaacag agaaaaagga aaccucacac cauacagaua cuucaaaagg     1860

aaagcaucac uuacauggaa cuacgaggcu uauaaaaguu acgugauaga auuauauaaa     1920

gacggaaaag gagaccucag cagaacaaaa uuagaacucc uacuauuuga agaagauaua     1980

aauaaguaug agguaagaca aagauucauc aauagaaauc ucgucgauac acgauaugcc     2040

aguaaagucg uacuaaauac uuuacaggac uucaugaaag aaaacuaccc aacaacgaca     2100

guugagguag uaagaggcaa auuuacugca caacuucgua gacacugggg aauccgcaaa     2160

aauagagacg aaaaucaugc ccaccacgcc aucgacgcuc uaauaauagc augcguaccu     2220

caccuauccc uauggaaaga acaaaagaaa guuuuuagua cagacgaauu uagaguagau     2280

gaaacaacag gagaaauaau cgaacaaagc uauaaucaac ucacauacca agaacccuuu     2340

ucacaauucu uuuaccaacu agaaaaaguc agcauagauu gcagauauuc cuacaaagua     2400

gaccgaaaau uuaauagaca aguagcagac gcaacaaucu auucuacacg ucaaggcaua     2460

guaaaacaaa caagugggga caaaaguuuu acgcuagaca gcaacucaaa agaacauuau     2520

gugaucgcaa aaaucaaaaa uauauaugaa gacgucgaug ccaagagauu cauggacaga     2580

uacaagaaag acaaaacgca auuuuuaaug uaucaccaug acccacaaac auuuagcaua     2640

cucgaagaug uaaugaaaac auauccagac aagaaaaauc cauuccaagc auacagauua     2700

gaauauggac caauacgaaa auauucuaag aaagguaacg gcccacaaau ucaaucgaua     2760

aaauauuaug auucaaaacu aggccaacau aucgaauuaa aacaagccaa ccaaucaacc     2820

aagaaaguag uacuacagag cuugaauccc uggagaugcg acauauacua uaauaagaaa     2880

gacaaacgau acauugccgc gggauuaaaa uacgcggacc uuucuuauca aaaaggaacc     2940

gguuuauacg gcaucagcuu acaacgcuau caacaaauac ucaauucaga gaaaauagaa     3000

cuuucaauag aacaaauaga acuacuucuc gaaggaagau ugaaugaaag caauuucgag     3060

uucugcuuua guuuauacaa aaacgauuuu auacaaugga acgaugaaaa uaaccaaaca     3120

uuucaauaua gauuucuugc acgaaacuua ucaucaccaa acaggauaga aguaaaacca     3180

auaaauaaaa guaaauacga caaacaagca gagggacuaa aaacacuaaa gaaaggacug     3240

aacagguucu acaaaauauc ugucgacgua cuaggcuauc aacauuuuau aaagaaagaa     3300

aaauuaaaac uaacauucaa auga                                            3324


<210>  5082
<211>  3330
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5082
augagccacu auguauuagg auuagacaua ggaauaacaa gugucgggua cggcauaauu       60

gaucuagaua caaaugacuu cauagacuac ggcguuagac ucuuuaaaga aggcacugca      120

gcaaacaacg aagaccgcag aaacucaagg agcagaagaa gacuacuauc ccgaaaacau      180

acuagauuaa uggacaugca aaaaguauua aaagaagcag gaauaaugac ugaaucauau      240

ucaccacuaa guaauguaua ugaaauccgu aaaaaaggac uaacuaaaga acugaccaac      300

gaugaacuaa cugcagcaau acuacacaua acaaaacaca gaggaucagu uaucgaaacc      360

guugaagaug acgaagccgc agccaaagag acuggagaau uaaaauccac ucuacacgaa      420

aacgcacuac ucuuagcuaa uaacaaauuc guaugugaaa uccaauuaga aagauuaaac      480

accgaaaaua aaauuagaaa ucauaaaaau aauuuuaaaa caaaggauua cguagcugaa      540

cucaaagaaa uacuaaacca ccaaaaccuu agugaggagg uaaaaaacaa aaucauacaa      600

auaguugaac gcagaagagc cuacuacgaa ggacccggcu cauuuaacuc ccccacccca      660

uaugggaggu ucauagaagu aaacggacaa guccaagaaa uugaccuuau agaaaaaaug      720

agggguaaau gcucugucua cccggacgaa uuuagagcac caaaaauguc cauaagugcc      780

gaccuuuuca acauacuaaa cgacuucaau aaucuacuag uacaaggaga aaaacuacaa      840

gaagaagaca aaaaaaaccu auugaaaauc aucaacgaca aaggaucuau aacccuacga      900

caaauaucaa aaaaauugga aguuaaugaa gacgaaauaa aaggauaccg uauagacaaa      960

aaaggaaaag cacucuucac ugaauucaaa ggauacaaga ucuuaaagaa acuguuuuua     1020

aauuguggac aaagcauauc acuggaggau uaugaaaugc uagacgaaau aaucgagaua     1080

guaacuaaaa aaaaaggaau cgaugaaaga aaagacgcac uaaaagaacu caaaugggac     1140

uuaagcgacg acauaauuaa cucauuaagu gaaguaaccg gcauaguagg cuaccauagc     1200

cuguccuuaa aagcaauacg agaacuggau ucagaacuau acaaaacauc auuaaaucaa     1260

augcaauuac uucacgaacu gaaaauguuc gauaaaaaca gaccaagcua uaaaggcaaa     1320

aagaacauag uugcagaccc cgaagcaaua uuaagcccag ucgcaaaaag agcacaaaac     1380

gaaacauuca agguuauuaa ugcacuaaga aaaaaauaug gagaguucga cagcauaguc     1440

guagaaauga cuagagauaa gaacucacaa gaacaaaaaa aaagaaucaa cgacagccaa     1500

aaagcauuag aaucgcuaaa caaagaagua gaucaacuac uccaacaaaa gggcuacgau     1560

ccacaaaaag ucaaagggaa aacaaaacaa aaggucagau uauauaugau gcaagaaggu     1620

aaaagcgcgu auacauuaca agaauuggau uuacacagag ugaucaaaga cgacaaauau     1680

accgaaauag accauauaau accaauaagc auuucauuag augacaguca aagcaauaaa     1740

guacuugccu uacacagcga aaaccaacaa aaaggaaauc uaacccccuu cauggcuuuu     1800

cauaccggaa gauucgcgga caugggcugc acguggaaua aauuuaaaga aaauguacua     1860

uccaacaaga acuacaaaca uaaaaaaaaa aacaacuuac uagaugaacg agacauaaac     1920

aaauucucag uaauacaaga auuuauaaac cgaaaccuag ucgacacuag cuaugcaaac     1980

agaguaguac uaaacacucu uacagacuau uucaaagaca augaaauuga uaccaaagua     2040

cauacaguaa acggaaaauu aacggauuua uuuagaaaaa gaauaaaccu aaaaaaagau     2100

aggaacgaag acuaucuaca ccacgcaaua gacgcccuga uagucgcaag caucaaaaaa     2160

cuaagacucu uggacggaua ccuagcaaaa uauggcuucg aaacacuaua cgacgaaaug     2220

acaggagaaa uaaaagaaau uccagaagau aaagcuuuuu uugaugacaa auacauacga     2280

uauauauuaa accucaaaac aaucuaugaa aguagcaaca uguacuauag aggaauacua     2340

gaaagagaag aauuaauaua ccaacccaua aaaauaucac auaaaauaga uaccaaacca     2400

aaucgaaaag uuucagacga aaccauuuac ucgacaagaa uaauugacaa cgaggaucac     2460

cuaauugaaa aauauucaga cauauacgac ccaaaauuua aaaaccucac agaagauauc     2520

cuaaauggag aaaagaauaa auauuugaug gagaaaaaug auccccaaac guucaauaua     2580

cuaaaacaaa uaauauucaa ccacuacaac acuuacaagg acucaaaaga auacuauaaa     2640

auagagaaaa agaaagaaaa agaaauaaua aaacuaguag gaauuaaccc ccuuucuuua     2700

uacaaagaag aaaacggccc cauuaaaaaa uacucuaaaa aagguaacgg accaguuauc     2760

augucaauga aguacgacuc ugaaaaauua ggcaaccaua uugauauaac accagacucc     2820

gcgaaaaaua aaaagguuau ccucaaacaa aucagccccu aucgaacaga uuuuuauaaa     2880

ugcagcgacg aaaaauucag auuuguaaca guacgcuaca aagauguaca uuaucaaaaa     2940

ucgauagaua aauacugcau ugauaaggaa ugguaucaaa acgaaaaaaa aaacaaaaaa     3000

auagacgaaa aagcagaauu uuuauuuucc augcaucaug acgaacuaau agguaucacu     3060

agaaaagaau cggaacaauu caucuacgac gaaucuaaag agaaccacgg agacccgcga     3120

uaccacgacg gaguaaaacc cgaaauccua aaauucacag cuaccaauaa ugauacuaaa     3180

ggaaucauug aagucaaacc acuauauacc uauucagcuu acagacuuaa accuacugua     3240

cuaaacguga aaaacauaaa aaaauacgca accgacguau uaggcaaccu cuacgaagua     3300

aaacaaaacg aacucaagcu agaguuuuga                                      3330


<210>  5083
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5083
auggcagaag guaagaaacg aauauggaga cuuggcgcgg auuuaggugc aaauucccug       60

ggaugggcag caauagaacu uagugcacca guaaauggaa aaccaaucgc aaucuuggca      120

gcuggcucaa ggauauucuc agauggacgu gauccgaaau ccggagcauc auuggccguu      180

gacagaagag augcaagagc aaugagaaga agacgagacc gauucaaaca aaggcaaaag      240

gcuuuaauaa aguauuuaau ugcugacggc uuauuuccag cagaugaagc agcacgaaau      300

gcuauggcug cgauagaccc auacgcaguu agagcacgag cacuagacga ggcuuuauca      360

cuacccgaau uggggagagc ucuuuuccau cuaaaucaac gacgaggauu uaaaucuaau      420

agaaaagcag auagaaaagc ggacgaugac gcaggaaaga uagcaauagg aauagaucgc      480

acaagagacg caauagcaga agaugaagca gacgcaggag cagaaccugg aagcuggaca      540

uucggcaagu ggcuucauga gagaagacua aaaggacaaa guguuagaac aagacuucgc      600

ccagaaucag gcgaagacgc caaaggagac ggcuaugauu uuuauccaga ccgugcucuc      660

auggaagaag aguucgaugc aaucugggca gcacaagccc cucaucaucc agauguccua      720

acaaaugaug uccaugauag auuacacgaa aucauauuuu aucaacgacc ccucaaagcg      780

ccacaagucg gaaugugcuc gcuaauuccu ggagauagaa gacucccaaa agcccauccc      840

uuauuucaac gccgaagguu acuggaagaa cuaaaugccc ucaugauagu acgaccagga      900

gaaguugcac aacgucuaac aagagaagaa agagacauac uuuuacuaaa acucaaagau      960

aaaaauaaag ucucuuucgc aacucuaagg aaagcucuaa aacuagaucc cgaugcaaga     1020

uuuaauaaag aaagugaaga cagaacagau uuaaaagggg augaaguagc agcuguuaug     1080

ggaucuaaaa caagauucgg aaacuuaugg guucauuuaa guauagaagc ucaaugggau     1140

guacuagcaa gacuagacgc aguugaaagc gaugcggaag augccgcuuu cugugcaugg     1200

uuaguugaaa cguaugcgcu gacaccagaa caagcaaggg caguagcaaa ugcgccuuua     1260

ccaccagggu acggccgguu cggagaaacu gcgacaagaa aauuaauuga agcacuaaga     1320

gacggaaaga aggacgaucg agucuuagua uacagugaag ccgucacuga agcuggcuua     1380

ggccaucaua gcgacagaag aacaggagaa guauuugaag acgcgaaagg aaggccgcaa     1440

uugcccuauu auggaguggc auuagaacga cacauaaugc cagggacagg agauccaagc     1500

gacccagaag agaugcgagu cggccgacua acuaauccua caguacauau uggacuuaac     1560

caguuacgaa gaauacucaa uguacuuaua agagcguaug gaccgccagc agaaauugca     1620

aucgagcugg caagagaguu aaaacuaaca gaugaagaua agaaaagaau aaacagagag     1680

aacaacgaua auagaauagc agcagagaga cggagcgaaa aauugcucgc aauaggacaa     1740

gcagacucug gagcaaaucg agcauuacug aaacuauggg aggaacuaaa uccagcaaau     1800

guacuagaua ggagaugcau auauagugga agacaaauaa gcaucgacau gcuuuucuca     1860

gcugcuguag aaguggacca cauacuuccc uuugcugcaa cacuagacga uuccaaugga     1920

aauaaaauag uuuguuuacg agaagcuaau agagagaaaa gaaaaaggag uccacacgac     1980

gcaugggggc auagugcacg augggacgaa auagcagcca gagcagcuca acuaccaaga     2040

aauaaaagau ggagauucga accagaugca auggauaaau ucagagauga aggugguuuc     2100

caagcucgac aucucguaga cacacaauac cuagcacgac uaagccgaga auauuuagcc     2160

acguuguauc cagaaaaagg agaaggcuca ggacauauuu ggguaucgcc aggaagacua     2220

acagaaaugg uacgaagaaa auuagguuua aacgaccugu uaccggauca caacuuugga     2280

ggaggagcgg aucaagcgaa aaacagacuc gaccauaggc aucacgcaau ugacgccgua     2340

gucguagcga uaguggaccg gggaauguug aacgcuauag caagggcuag uggucaagaa     2400

ggagcugaag gaagagaaag aauaauaauu ccagagccau gggacggguu uagagacgcu     2460

cuacgagaga ccguaaaucg caucaccgua ucacaucgca ccgaccacgg cacagcauca     2520

aaagcaggau uaccaaaagg aaaagacgca acagcaggac gauuacauaa ugacacagca     2580

uauggauuua ccggagacac ggaugcaaaa gguaauagca ucguuguaag aagaguacca     2640

cuaucaaguu ucaagaaauu agcggaucua gaucguauac gagaugcaga ucuaaaagcc     2700

gcauuguauc aacaucuaaa cggacuagaa ggaaaagcgu uugaaaaagc auuggcugag     2760

uuccauaaau uggaauggca caauuuuggu ggcguaagaa gagugagagu aacugagcca     2820

uuaacaguua uuccaauaaa agauagaaau ggaagaaucu auaaagggua uaaaggugac     2880

ucaaauuaca gguaugaugu augggaacua ccugacggca aaugggugac caauuggaaa     2940

aacgaagcag gggaagcaau gaguagugua auauccaugu uugacgcaca ccaaucauca     3000

gaacccccaa gaccacaccc agcagcaaga aaaauauuaa gucugcauca aaaugaccua     3060

uuagcuauug aaagagaggg agaagauaga aaacuuaugc guguaguuaa auucggacaa     3120

agcgggcaga uuacacucgc cgaacaccaa caagcagggg aucuaaagaa acgagaugca     3180

guaagcaaug augacgaccc uuuuaaauau uucgcaccga caaguggagg cuugaagaaa     3240

gucaaagcga ggcaaguaag aauagacgaa auagguagag uacuagaccc aggcuuuccg     3300

gcaagaacug cgagaagaaa aacgcgaggg uga                                  3333


<210>  5084
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5084
augacggcaa gaacucauaa aauauggcga uuaggggcug aucucggagc aaacagucuu       60

ggaugggcag ccauagaacu aucggcaccu guagauggaa aaccaaucgc aauacuucau      120

gcagggagcc gaauauuuuc agacggcaga gaucccaaau cgggcgcuuc auuagcagua      180

gauagaaggg augcgagagc uaugagacga aggcgagacc ggcuccaaca gagacaaaga      240

gcacuuauaa aauacuuaau ugcagauggc cucuuuccag cugaugaaaa cgaaaggaaa      300

gcaauggcaa caauagaucc auacgcaguc cgcgcaagag cacucgauga agcucuaacg      360

uugcccgaag ucgggagagc acucuuccau uugaaucaaa gaagaggauu uaaaaguaau      420

aggaaagcug auaggaaagc ugaugaggac gcgggaaaga uugcgauugg aauugacaga      480

acccgagaug cuauucgaca agcagaagaa gaagccggug cugaaccagg cucauggaca      540

uaugguaaau gguuguauga gaggagagcg uccgcaacag acgcaaaugc aauaccaucg      600

gugagaacga gauugagacc ggaaacugac gaacacgcua aaggggaugg auaugauuuu      660

uauccuggga gagccuuaau ggaagcagag uuugcagcaa uaugggaagc ucaagcagca      720

caucauccag acgcguuaac aaaugaggua caugauagau uacacgaaau aauuuucuac      780

cagagaccuu uaaaagcacc gcaaauagga guauguacau uaauaagagg agaucaaaga      840

cuuccgaaag cacacccccu auuucagaaa agaagacucu uagaagaauu aaaugcauua      900

acaauaguaa gaccuggaga gauggcccaa cgucugacaa gagaggaacg cgacacacua      960

uucuugaaau uaaaagauaa agccaaggug ucauuuguaa gucuaagaaa aacacuaaag     1020

cuagacccug acgcaagauu caacaaagag ucagaaaauc gauccgauuu aaaaggagac     1080

gaaguagcug cagcaauggg ugccaagacg agguuuggaa auagaugggu gcauuuaagu     1140

auagaggaac aaugggaugu cauagcgcgu cuugaagaag uagaauccga ugcagaagac     1200

gcagcaugua gagcaugguu agccgcaacc uuugauuuaa caccagaaca aaucagagca     1260

guaacuaaug cuccgcuacc accagguuau agcagauuug guaaaguggc aacacaaaga     1320

cugauugaug cacuccggga cggagaaaaa gaugguagag uauuaguaua uucugaagcu     1380

guagcggcgg caggauuagg acaucauagu gaucuaagaa caggugaaau auuugaagau     1440

gcccaaggac ggccacaacu cccauauuau ggaguugcac uagaacgaca uaucaugccg     1500

ggcacaggaa aucccgauca uagugacgaa augagaauug guagacuuac uaacccuaca     1560

guacacauag gacuaaauca auuacgaaga auaguuaaua cauugauacg agcauaugga     1620

ccgccggcug aaauugcaau agaacucgcc cgugaacuaa aauugacaga ugaugacaag     1680

aaacguauua auaaagaaaa uaaugaaaau agauuagcug cggagaaaag aagugaaaaa     1740

uuauugacaa uaggaagacc agauacagga gccaaccgag cauuacugaa auuaugggaa     1800

gaauuaaauc cugcaaacgu guuggauagg cgaugcauau acacgggcac ccaaauaagc     1860

guugauaugu uauucagcgc cgcugucgaa gucgaucaua uacuaccuuu caccgcaaca     1920

uuagacgacu cgaaugggaa uaaaaucguc ugcaugcgag aagccaauag aacaaaacga     1980

aaacaaucac cauaugaggc uuggggucau acugacagau gggaagaaau agcagcaaga     2040

gcagcacaac uaccuagaaa uaaaagaugg agauucgaac cugacgcaau ggaaaaauuc     2100

aaagacgaau caggauucca agccagacau uugguugaua cacaauaucu agcuagauua     2160

ucaagagaau auuuggaaac acuauauccu ggaucgggag aaggaaguuc acauauaugg     2220

gugagcccug guagauuaac cgaaaugguu aggcgcaaau uaggauugaa ugauuuacua     2280

ccugaucaca acuuuggggg aggugcagau caagcgaaaa aucgucuuga ucacagacac     2340

caugcaauug augcaguagu aguagcgaua guggacagaa guauguuaac cgaaauuuca     2400

agaguuagug gacuagaagg agcugaaggu agagaacgaa uaaucauacc accaccaugg     2460

gaugaauuua gaaaugaucu uagaacugca gugaacgcaa ucacagucag ucacagaacc     2520

gaucauggga caauaaguaa agcugguuua cccaagggca aagacgccac cgccgguaga     2580

cuucacaacg auacagcgua cggauuuaca ggugaaacgg acgcaaaggg caauuccaua     2640

guaguuagaa gaguaccauu ggcuucauuc aagaaacugg cagaucuaga cagaaucaga     2700

gauacagauc uaaaagcagc auuauaccaa caccuugaag gacuugaagg uaaagcauuc     2760

gaaaaagcug ugauggacuu ucccaacuua gaauggcauc cuuuuggaaa ugugcguagg     2820

guacggguaa cagagguauu aaguguaauu ccgauaucug accaaaaugg uagaauuuau     2880

aaaggauaca aaggggacag caacuauaga uaugacgucu gggaauuacc cggaggaaaa     2940

ugggaagcug aaguaguaag cacuuucgau gcgcaucaac cggacugggu aucaaaaaua     3000

agaagucgua guccuacagc cagaaaaguc cuaucauuac aacaaaauga ucuuauagca     3060

guagaaagag cgcacgaaac uagagaacua augcgaguag uaaaauuuag acaaaaugga     3120

gaaauuaccu ucgcaccgcc acaagaggca ggaagucuga aaagaagaga ugccacuccc     3180

aaugaagacg acccauuuaa auacuuugcu guugcagcau caaguuuaca aaaagccaaa     3240

gcuagacaag ugagaauaga ugaacuggga agaguauugg aucccggauu uccagcacga     3300

acagcgagaa gaaaaacaag aaaaucaaca uga                                  3333


<210>  5085
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5085
augaggauac ucggauucga uauaggaaua acaucaauag gaugggccua cgucgaaggg       60

gaagaauuaa aagacugugg aguacgaaua uuuacaaaag cagaaaaccc acaaaauggc      120

gacucccucg cagcucccag gagagaggca agaggcguga gaagaagacu cgcacgaaga      180

aaaguacgac uaaauguaau aaaacggcua cugugcaaag aauuugguuu gaaccuaagc      240

gauuacuucg caaacgaugg agaauuaccu aaagcauaug aaacaacuaa agauaccaaa      300

uccccguaug agcuuagauc ucuagcguua gaacaaaaau uagaaccaaa agaacuagca      360

cgugugauuc uucauauugc aaaacauaga ggauacggca auaaacaugc aaaaagggau      420

cuggaggcug aagagaaagc aaagaaagcu gccgaaaaag caccacaaga aaaagaaagu      480

acaacaacaa auggagacuc aaagaaagaa caagaaaaag uacuaaaagc auuauaucaa      540

aacgaaacuc uccuaaagaa auaccaaaca aauuaccaaa cuaacaauaa aacuaacgau      600

gagaccaaca acaaaaccau auuucuagug ggaaaauacu uauacaaaga guuccaagau      660

aaaggacaaa gaauuaggaa cacaaccaac aacuaccaac acaccaugag acaagaaugg      720

cuuaaagaug aacuagaguu caucuucaag aaacaaaaag auuuuggagc uacauucagc      780

gaaaacuuug aaucacaaau ccucgaaaca gcauucuacc aaagagacuu aaaaucauuc      840

gaagauaaag uaggaaaaug uguauucuac gaaaacgaaa auagagcgcc aaaagauuca      900

cugucggcua uggaauuugu agcauuaacg aggauaauaa acacauuaaa gaaucuagaa      960

aagaaaagua aaaacuuagg cauaggcgaa acuuauggca aagauaaaau acaagaaaua     1020

cuaaaaauag uauuggacaa aggcgaaaug ucauacaaag aacuacgaaa aauauuaaaa     1080

cucaacgagu cgauacuuuu cgcaaaagac agcaaacuug auuacacgaa agguaugcaa     1140

gaggcagaaa aagcuaaauu cauagaauua aagaauuuaa aagcauucaa gaaagcuaug     1200

ggaggagacu uuagcaaauu cccaagagaa gaacuagaca aaauugcuac agacauagua     1260

cuaauaaaau caaaagaaaa ccuagcaaag aaacuacaaa acuauagugc acuaucaaaa     1320

gaacaaguag aagcacuauc aaaucuaaau uuugcaaaac auauagauuu aaguuuaaaa     1380

gcccucgaca aaauacuacc cuuuaugaga gagggcuuaa guuacgacga aguuguaaag     1440

aaagucggac uccaagaaca cagaaaauau aaacaaaaag gcgaguuuuu aaucccauua     1500

aaagaauacg aacccuaucu agcaaaucca guaguagcac gagcacucuc cgaauacagg     1560

aaagucuuaa acgcgcuguu acgaaaauac ggaaauguac acaaaauaca ucuugaauuu     1620

accagggaag cagguaaaaa uuacgaugaa agaaagaaaa ucgaaaaaga acaaagagaa     1680

cacuucgaag ccaaucaaaa agcgauaaaa caacgcgaaa ucuuaggacu cccaauaaac     1740

ggcgcuaaca uauugaaaau gaaacuaugg auugaacagg gagaaugcug cguauacuca     1800

ggggagaaaa uaacaagcaa acacuucuuc gauucaaacg ccuuacaggu agaccacaua     1860

uacccauacu ccagaaguuu ugacgacucg uauaugaaca aaguacuggu cuucgccaaa     1920

gaaaaucaaa acaaacuaaa caaaacacca uucgaagcau uuggaagcga caaagagaaa     1980

ugggacagaa uuuuaucguu aucagcgaaa cuuccgaaac agaaacaaag gagaauauca     2040

aauaaagacu uuaaagauaa agaauuaggg uuuauagcuc gaaacaucaa ugauacagga     2100

uacauagcaa gacuagcgag ccaauggaca aacaauugcu uaaaauucga ggaucuaagg     2160

gaaaaugagg uuacuauagc aggagaaaaa gggaguaaaa aucaucucga agucauauca     2220

gggcaauuaa cuucuauguu acgacacuau uggggccucg gcgauaaaga cagaaauaac     2280

caccuucauc augcuguaga ugcaguaauc auugcauaua cuaaugcaaa aacaaucaag     2340

gcuuuugcug auuucaagaa aaaccaagaa caaaauagag cuaaauuuua cgcaaaacag     2400

auaucagaac aggaauacaa aagacaacga aacccuuucg aaccuugcgc aaacuucaga     2460

caacgaguag aagagaaaau aaauaaaauc uuugucagca agccaccacg aaaacgagca     2520

cgaggagcau uacauaaaga aacauucggu agcaucugcu uagacggcaa uaauaacugg     2580

guagcgagau uuaagaucaa aaguaagaaa gaacaagaag auauuacaaa agaaaaagcu     2640

acgaaagcga aauacaaaga cgaguucaaa ggagaagaaa acauacgaaa agcgcuaaaa     2700

cuuggaaaaa ucaggagaau uaacaacacc caaaaagaua uugcaauugg agacaaaaua     2760

gucaaaaacg aaacgauggu aagaguagac auuuucaaag acaagaaagg aaaauucuau     2820

gcaguaccua ucuauacgau ggauuuugca cuaggcaucc ucccaaacaa agcaguagca     2880

acaggagaag augaagaugg uguaauaaaa gacuggaucg aaauggacaa aacuaaauac     2940

gaauuuuguu ucucauuauu uaaagacgac cugaucaaag uacguaaaaa gaaaaugaaa     3000

gaagcagaac uaugcuauuu cguuggauuu gauacagacg guucaagaau aauuguggcg     3060

aaacacaaca augauuauca aacaaugacg gaaaaugaau gcaaacuauu cacacuaaaa     3120

gaaaagaaaa agaaacagaa aacauuaaac gacgaagaag auaaccaaga aagaauagaa     3180

accgaauaca aaauaauagg aggaagaaca ggaauacaga accuugaaac auucgaaaaa     3240

caccaaguau caccacuagg agaaguccaa aaagcagagu ucgaagauag acaggaaaua     3300

aaauugaaau ccucuccaaa aggcaaagau uga                                  3333


<210>  5086
<211>  3333
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5086
augucagaga aauaucgcuu aggacuagau guugggacaa acucucuagg augggcagua       60

auaggacuaa aggaagacgu acccucuuca guauuagcaa cagggguaag aauauuuagu      120

gaaggaagag acgccaaaac gaagucaacu uuaaaagcua gucgaacaga gaaacguuca      180

gcaagaagac gacgagaucg uuuucuacaa aggcaaacau uuuuaauauc cgaaauggua      240

aagucagggu uguuccccaa agauauagua gaacaaaaga accuagaauu auuggaucca      300

uaugaaauca gauuuaaagc cuuaacagaa caaguaccgc uucaccaccu agguagagca      360

cuguuccaca uaaaccaaag gcgugguuuu aaaagcucaa gaaaagauaa aagugaagaa      420

acaacaucag guguaguaag uaagucaguc agagccuuau augaagaaau gcaacugaua      480

aauccagcgc caguuauaga ugauccuaaa gccuugagua aagaagaacg uagagaaaua      540

agaguacaag aagcauccag ucgugaagaa gcauuaguaa cccuagcaga acaaaaaggu      600

guaaccuuug gugcauuucu auacagaaga uuacaaagcg guaaaagcgu ucgagcucgu      660

aaagaugcag auucacaacu uuaugaugua uaccccacac gagaacuucu caaagacgaa      720

uauagcaaau uaaugcaaca ucaggcccag uacuacccua auguaauuaa cgaagcacua      780

auaucaagac uacacgacgu aauuuucuuu caaagaaagu uaaaagaaca agaaagaggu      840

uucuguacau acuacccuuc ugaaagacga accuuuagau guaugccauc guuccaacaa      900

uaccgaaugg uacaagaacu aaaugcauua gaaugggucg augcggccgg aaagcauugu      960

uuacgagacu accccgaauc uagagacuua auacuaucug aauuagaaaa uauaaguaca     1020

aaagcaggag aguucacauu cgguaacaug aagaaaauau uaaagaaaau ggguguaaua     1080

gacggagaag uuagcuucaa uuuugaggga cccaaaagaa aaggauucca gggaaacgua     1140

acaucucgcg aaaugcgaca ugaagacugc uucgguacua gaugggauga acuaucacua     1200

gaugaaaaag acgacaucau ugacaaaaua aaugaugauc acuuggauga cgaagauaug     1260

gaaagguauc ucauagacac auauggacua ucaucauuua guguaggaaa ugcuauaaac     1320

gcccgccucc ucgaaggaac agcaaacgug ucaaggaaag ccgcaagaac auuauaugaa     1380

acaaugcucu cggcgaauug ucuucaauca gaugcaguca uagcaguauc agaacaagau     1440

gauacauuug uaaauccaua uagcagaaca ggcagaggug aacuacuaag ugaguuacca     1500

uacuauggag aagcauuuca agaugguaga cauauaauac cagguaaaag agaaccaaag     1560

gucucacaug aucaacuaaa auucuaugga gguguuagua auccuacugu ucauauagcu     1620

cuaaaucaaa uaagauuagu aguaaacgaa aucaucaaac guuauggucu accgucaucu     1680

auuaguauug agcuugcuag aaauuuacca gaaggacagg auggacggcg gaaaauagag     1740

aaaaugcaaa gcgaaaauca gaaaaacaau gaaagauacg accaaauuuu aacagaaaac     1800

ggacaaagcc cuaaucggga caaucggcua cgacucgccc uaugggaaga auuaggcgaa     1860

gguccaacug acagauuaug cguauuuaca ggaaaacuua uagaauuagc agacuuauuu     1920

aacgguucug uagaaauaga acauuuaaua ccauuuagug uuagucucga ugauucaaaa     1980

gcaaauaaaa cagucuguug gaaagaagca aauagauuua aaggaaacaa gaccccauau     2040

gaggcauuug caaauaacag cggagauuau ucaugggaag agauaaugga aagaguaaga     2100

gcauuaccca auucaaaaca auggagauuu caagaggaug cacuaaaaau uuggaacuca     2160

gaucauucag auuuuucugg acgacacuua aaugacacua gguauauaag ccgacuggca     2220

agagaauauc ucgaaaauau uuguccauuc aauaagauag acgucguuac aggaagauua     2280

acagcacuau uacgaggaca uuggggucuu aauaauauac uacgggguca uaacgaaccg     2340

gaaacagcua caccuaagaa aaugagagau gaucauaggc accaugcuau agaugcugua     2400

guaauagcaa ugacaucauu aacgauauua caaaaaguua gcagcgcugc aggcaaagca     2460

gaggaacucg auguaggaaa auuauucgca aaagaugaaa guggaagaag cguaauagac     2520

ccaugggaug gcuucagaga cgagguggcu gaaaaaguuc ggaauauaau aguuagccau     2580

aaaaccaaaa gaaaaacuau aggcaauagc ggaaaaucaa cagauagagc gcuacacaac     2640

gaaacggcuu augguuugau agacggaccg gacgagaagg gacuauacag cguaguuacc     2700

acaaaaccaa uuuccuacuu ugauaauaga gacaaaauag aaaagauaag agaugaaaaa     2760

uuaagagacg auuucuuaac cgcaugggau ggagaaggug acaauaaagc cucucaauua     2820

gcuguauuag acaaagcaag cggucuaaau guaaagaaau gucgauaugu cgaaagaaag     2880

aaaguuaucc ccauuaaugg acccgaugga caaccauaca aagccuauga cggaaauagu     2940

aauuggggua uggaaauuua ugaguuccca aauggacauc cuaaagaaaa gaaauggaac     3000

ggaguaguua uuagcacgua cgaugcuaau aaaucagaau uuaaaccagg agaaacauuc     3060

agaccacacc cagcagcaaa auuaguaaug agacuacaua uuaaugauau aguagaggua     3120

caaaacgacg gagaaccuau auuauaucga guacaauuaa uaucggcagg aacacucacg     3180

guuuccccau uacaugaagc uaacguagau agcagaaauc gagacaaaca cgacgaauuu     3240

aaauacaaau cauuguccgu uaccccgaug cagaaaaugg gagcgaagaa aguaaauaua     3300

ucaccaacag gacuuaaaaa cuauuccagg uga                                  3333


<210>  5087
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5087
auggguauaa uaauaggacu agacauaggg aucgcuagug uaggaauugc aguaguggac       60

aaagacaccc uagauauaaa augcgucgua agugacuugu ucgauucagc ggaugcuagc      120

aaaaauauag aaagaagaaa caauagacaa gccagaagau uaaauagaag acgaaaaacu      180

agaauuggag auuuuaauac ccuaugggua ucaauguuug gagagcuccc agaagaaguu      240

gauucaaaug ucuuacuacu aagaaaugca ggacuagacg aacaacuaac acuagaugaa      300

auauauugcg uccucaagua uaugcuaaaa cacaggggaa uaucauaccu ggaagaugcu      360

cuuaaugaag aaaacguaac aggaaguuac cagaaaggga uugcaauaaa ccagagagaa      420

uccgaguaca ugcuaccuug cgaaauacaa cuagaaagau uccgaaaaua uggacaauac      480

agaggagaau gcgaggcaga aaacgaaaac ggugaaaaga uaacccuaag caacguauuu      540

augacuaauu cauacagaaa ggaaauagau aaauucuuaa auacacaagg aaaauacgga      600

auacuaaacc aaaaauucau agacgaauac cuaaaaauau ucaaucggaa aagaaaauac      660

uaugaaggac cugguaauga aaaaucaaga accgacuacg gaaaauacac aacaggaaaa      720

gaucuagaug gaaaauacau aacggaaaaa aacauauucg aaaaauugau aggcaaaugc      780

uccguauaca aagaagaauu acgugcagcc ggagcguccu acacagcaca agaauuuaau      840

auucuaaaug aucuaaacaa cauaacaguc aacaacaaaa aacuaacaac ucaacaaaaa      900

aaagacguca uugaaauagu acaaaacucu gaccgcauua acauggaaaa aaucauaagc      960

agcugcauag gagaaaaaau cgaaaaaaua gaaggagccc gaauagauaa gaacgaaaaa     1020

aauauauacc acaccuuuga acaauauaac aaaaugagga agaucuucgc ggaaaaaaac     1080

uaugacauaa aagaacucac aauugaacaa cuugacgaga uugguagaau acucacaauu     1140

aacacagaga gagaagggau uaaggaugca aucauggaau cagaacuacu acaccucaau     1200

aacgaacaaa ucaaccuucu cauagaacua agguacaaaa auucaucacu cuucacaaaa     1260

uggcaaagcc ugucaauuaa agcaaugaga gaaauaauac cugaccucua ugauagaaau     1320

gugaaccaaa ugaauauacu ugccgagaga ggaguauuua aggacaaaau uaaucuguuc     1380

gaaaacuccg uauaucuacc aaaagagcca guacuagaag aaauauauaa ccccgugguu     1440

cgaagaucaa uaucuaugac cauagacgca uugaacgcac uaauaaaaaa auacggauac     1500

ccugaagaua ucguaaucga aaugccaagg gacagaaaug acgcagaaca aaaagacaga     1560

auaaaaaaau uccaaaaaac aagagaaaac gaacuaaaaa acauaaagaa aaaaauaaaa     1620

gaagaauaug gcauagaaau aacagacgaa uacuuccgca aacacaaagg auuagaacua     1680

gaacuaaaac uguggguaga acaaaacgaa aaauguccau acucaggaaa auacauaaac     1740

guacaagaua uacucuacca ccaugagcuc uuugaaauug accacauuau accccucucu     1800

auaucauacg acgacucaag aaauaauaaa guacuaguau auaaagaaga aaaccaagac     1860

aaaggaaauc gaacaccucu aauguaccua uccacgguaa acaggcaaua cgaccagcau     1920

gaauucauga acuacguguu auccacaaaa acacuaccac gcaaaaaacg agaauuauua     1980

uuaaacuacg aagacauaac aaaaauagau guacuuaaag gauuuauaaa uagaaaucua     2040

aacgacacga gauacgccuc aagaaguguu uuaaaugcaa uucagcuguu cuuuggaggc     2100

aaagaaugug acacaaaagu aagaguaauc aggggagccg uuacaaacca acuaagaaua     2160

aaacuaaauc uaccaaagga ccgcgaaaaa agcuacucuc aucacgcuau agaugccgca     2220

auuauguguu auucgcaaau gggcuuaaug aacuucagaa acauccaaaa caaauucaua     2280

gauuucgaaa acgaaacaua cuuaagugac gaacgcgacu cgugcuucga uaacaaauac     2340

uacgacgaag cacuauuugg acaaguguua aaaaugagga aaaacuuauu aaaagcagag     2400

gguaaaccac aaagaagugu acaaucaaaa ggaguauucg acgaagaaga aaaaaacaac     2460

ggaauuguac guuaucauca uaaaguggac aaaaaaccaa auaggggucu cucuaaugcg     2520

ucaauauacg gaacccgcaa aauugacgac aaaauauaua aaauaguagg auaugacaua     2580

agaaauaaca aagacgcaga agcaaucauc aauauuauaa aaaaagggaa aagcgauaau     2640

uucuugaugg caaaacacga cccagaaaca uuuaaaaauu uaauggaaau uuaugagcaa     2700

uacaaagaag aaaaaaaucc uuuuuuggca uacgaacagg aaaccggaga uuacauaaga     2760

aaauaugcua aaaaccauaa uggcccaaua auuaaaaaaa uuaaguacgu cgauuccgag     2820

guaggaucau guaucgacau aagcgcaaac uacgguuaua aaaauggaga uaaaagaguc     2880

auaauggaua cacuaacacc auacagggca gacguuuauu acaacuacaa agauggaacc     2940

uaccgaauaa uaggaguaaa auaugcggac uuuaaauaca guaacgcaaa auacguacuc     3000

gaugaagaag cuuacgcccu aauacuaaua aaagagggau uacuuaagau aggcgauagu     3060

cuugaaaaca uaucaagcuc uggauaugaa uacagacuaa gcuucuauaa aaacgacaua     3120

aucgaauaua ccaaaaacga uauaguauau aaagaaagau ucuuaagucg aacaaugcca     3180

aaaguaaaaa acuauaucga aacaaaaccc auuucagcgg cauuauacga aaaaagaaac     3240

ccaaucggac uaaccaacgc uacgaauaua uguaaaauuu acacagacgu ccucggaaau     3300

gaaacaaaga ucuacaaaga aaaauucaaa cuuucagucg auuga                     3345


<210>  5088
<211>  3345
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5088
augaauuaua cagaaaaaga aaaauuguuc augaaauaua uauuggcacu agauauagga       60

auagcuucag ucggcugggc aauauuagac aaagaaucug aaacaguaau agaagcaggg      120

agcaauauuu ucccagaagc gucagcagca gacaaucaau uacgucgaga caugagagga      180

gcaaaaagaa auaaccgcag auuaaaaaca agaauaaaug acuucaucaa acuaugggaa      240

aauaauaauu uaucaauacc ccaauucaaa ucaacagaaa uaguaggauu aaaagucagg      300

gcaauuacag aagaaauaac gcucgacgag cuauacuuaa uacuauacag cuaccuaaaa      360

cauagaggga ucagcuauuu agaagacgcg cuagacgaca caguaucagg uucuagcgca      420

uacgccaaug guuuaaaacu aaaugcuaaa gaacuagaaa cacacuaccc uugcgaaaua      480

cagcaagaga ggcuaaacac aauaggaaaa uacagaggac aaucacaaau aauaaacgaa      540

aauggagaag uacuugacuu aagcaauguc uucacaaucg gagccuacag gaaagaaauc      600

caaagaguau ucgaaauaca aaagaaauac cacccagagc uuacagacga guucugugau      660

gguuauaugc ucauauucaa ccgaaaaagg aaauacuacg aaggcccugg aaacgagaaa      720

agccgaacag auuauggacg auuuacgacc aaauuagacg caaauggaaa uuauauaacg      780

gaagacaaua uauuugaaaa auuaauugga aaaugcagug uauacccuga cgaacuccga      840

gcagccgcug caucuuacac agcccaggaa uacaacguac ugaaugaucu aaauaaccuu      900

acuauaaacg gcagaaaacu ugaagaaaau gaaaaacacg aaauuguaga acgcauaaaa      960

ucaagcaaua ccauaaacau gagaaaaaua auaucggacu guaugggcga aaacauagac     1020

gacuuugcug gcgcacguau cgacaaauca gguaaagaaa uauuucauaa auuugaaguc     1080

uauaauaaga ugaggaaagc auuauuagaa auaggaauag auauaaguaa cuacucaaga     1140

gaagaauuag augaaaucgg auauauaaug acaauaaaua cagacaaaga agccaugaug     1200

gaagccuucc agaaaucuug gauagaccua uccgacgaug uaaagcaaug cuuaauaaac     1260

augagaaaaa caaauggagc ucucuucaac aaauggcaga gcuuuucacu aaaaauuaug     1320

aaugaacuaa uaccugaaau guacgcacaa ccuaaagaac agaugacauu acucacagaa     1380

augggaguaa caaaaggcac ucaagaagag uucgcaggcc uaaaauacau accaguagau     1440

guagucagug aagacaucuu uaauccagug guuaggagau caguaagaau aaguuuuaaa     1500

auauuaaacg caguucuaaa gaaauauaaa gcacuagaua caauugucau agaaaugcca     1560

agagauagaa acagcgaaga acagaagaaa cggauaaacg acagucaaaa acuaaacgaa     1620

aaagaaaugg aauacauaga aaagaaacua gcaguaacau auggaauaaa auuaucuccc     1680

agcgacuuuu ccagucaaaa acaacuaagu cuaaaacuaa aacucuggaa ugaacaagau     1740

ggaauaugcu uauauagugg uaaaacaauu gacccuaacg acauaauaaa caacccacaa     1800

cucuucgaaa uagaccacau aauaccccga ucaauaaguu uugacgaugc aagaagcaac     1860

aaagugcuag uauacagaag ugaaaaucaa aagaaaggaa accaaacacc auacuacuac     1920

uuaacacauu cacauuccga auggucauuc gaacaauaua aagcuacagu aaugaaccua     1980

agcaaaaaga aagaauacgc uauauccaga aagaaaauac aaaaucuacu auacucggaa     2040

gacauaacaa aaauggaugu acugaaaggu uucauaaaca gaaacauaaa cgacacaagu     2100

uacgcaucaa gacucguacu aaauacaaua caaaacuuuu ucauggcuaa ugaagccgac     2160

accaaaguaa aaguaauaaa aggaucauac acucaccaaa ugcgcugcaa ccuaaaacug     2220

gacaaaaacc gugacgaaag uuacagccac cacgcaguag acgcaaugcu cauaggcuau     2280

agcgaauuag gcuaugaagc auaccacaaa uuacaaggag aauuuauaga cuucgaaaca     2340

ggggaaaucu ugcgaaaaga uaugugggau gaaaacaugu cagacgaggu guaugccgac     2400

uaccuauaug ggaagaaaug ggcaaacauc agaaaugaag uaguaaaagc agagaaaaau     2460

gucaaauauu ggcacuaugu caugaggaaa ucaaaucgag gauuaugcaa ucagacgaua     2520

agaggaacaa gagaauauga cggaaaacaa uacaaaauaa auaaacuaga cauaagaacu     2580

aaagaaggga uuaaagucuu cgcuaaauua gcauuuagca agaaagacuc agacagagaa     2640

agacuccuug uuuacuugaa cgaucgccgc acauucgacg accuguguaa aauuuacgaa     2700

gacuacagcg augcagcuaa uccauucgua caauaugaaa aagaaacagg agauauaauc     2760

agaaaauaca gcaagaaaca uaaugguccg agaauagaca aauuaaaaua caaagaugga     2820

gaaguaggug ccugcauaga uaucagccau aaauauggau uugaaaaagg uucaaagaaa     2880

guuauucuug aaagccucgu accuuaucga auggaugucu acuauaaaga agaaaaccau     2940

aguuacuauu uagucggcgu aaaacaauca gauauuaaau ucgaaaaagg acgaaaugua     3000

aucgacgagg aagccuacgc aagaauccua guaaaugaga aaaugaucca accuggucaa     3060

uccagggcag auuuggaaaa cuuaggauuu aaauuuaaau uaaguuucua uaaaaacgac     3120

auaauagaau acgaaaaaga uggaaaaaua uacacagaac gucugguauc aaggacaaug     3180

ccaaaacaga gaaauuacau agaaacuaaa ccgauugaca aagcuaaauu ugaaaaacaa     3240

aauuuaguug gccuugguaa aacuaaauuc auaaagaaau acagauauga cauacucggu     3300

aacaaauacu cuugcucaga agaaaaguuc acaucauucu guuga                     3345


<210>  5089
<211>  3348
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5089
augagcgcca cauauacaau uggccuggau auaggaauag caaguguagg cuggugcgca       60

uucggggaaa cacacauccu agaucucggu guaagggcgu uugacaaggc agaaacugcu      120

aaggaaggag aaaguuuaaa cuuagcacga agaaaugcca ggcucaugag acguagacua      180

uuucgacgua cguggcgguu aaagaaauua gcaagacaau uaaaaaguga agguuuaauu      240

gcugauacaa aauuguucaa accagaauua ccauucucaa agucauuaug gcaauugaga      300

guagaugggu uagaccgaag auuaaacgau gaagaauggg cuagaguuau cuaccauuua      360

ugcaaacaua gaggauuuca uuggauaucg agagccgaag caaaacaagc agaaggagau      420

uccaaaucag aaggcggaaa aguuaaacaa ggccuagcgg gcaccgcuaa auugauggac      480

gaaaaaggau auagaagcgc agccgaaaug guacucgccg aauuuccuca agcacaacgg      540

aauaaacaag gcgaauacca aaaagccuug agucgagagc uccuagcuaa agaacuugcg      600

cugcuuuuug cacaacaaag agaauauggu aaucuucaua ccccgggagc ucuagaaaca      660

gcuauccuag guaaugguga uaagaaauca ggguuauucu ggcaacaaaa accagcauua      720

gcuggcacag aucuccucaa aauguuagga acauguacau uugagaaagg agaauacaga      780

gcccccaaag caucuuuuac agcagaacga cauguauggu uaacuagguu aaacaauuug      840

cgaauuguca cagacggugu aaccagacaa cuaacagaac aggagcgaca acuauguuua      900

ccacuaccau auagacaggc uggagaccuc acauauaaac aauugggaag cgcgcuugcu      960

aaagcugguc uuuuagaggc aggcgguuuu aaauuuacag gucuagcaua uccaaccgaa     1020

gcacaaaaag cugaaggcaa agcaaaagau cccgaauccg ccacuuuagu aaaaauacca     1080

gcauggcacg aauuaagaca aacacuuaca aaagguggcu uaaaugacga augggaaaaa     1140

uuagcgggug augcccuaug cggacaacca gagauuuucg auaaaauugc augggucuua     1200

agugucuaua aagaugauga cgaaguuaga gccgaauuag ccaagcuggc ucucccuaau     1260

ccacaagcau uaaccgaagc gcuauuaaau auaagauuug acaaauuuca caguuuaucg     1320

uuaaaggcuu uaagagcaau agucccacau auggaacaag gauuacgaua ugaugaagca     1380

ugugugcaag cagguuauca ccacagucaa cuauuaaaag uuggagaagg agaacacaaa     1440

uaucuaccgc cauuauacga uggaagagau ggcgauggac gcaugauauu cgcagaagau     1500

gcagauauac ccaggaaucc ugugguccua cguucucuaa accaagcuag aaaagucgua     1560

aacgcaauua uucgacgaua cggcagucca agugcaguuc acauagaaau ggcaagagau     1620

uuaucuagac cauucgauga acgaaacaaa aucaagaaag aucaagauga auaucaagca     1680

cgaaaucaaa gagaucgaga acaauucagc gaacuauucg gacacccucc aaaaggucuu     1740

gaauuugaaa aauggcgauu auacagagaa caaaauggac aaugugcaua cucucaaaga     1800

ccuuuaacag cauccggaaa cguagcggaa auuuuccuug augguagcac agagauagau     1860

caugcauuac ccuacucacg uucuuucgac gacaguaaaa acaacaaagu ucuagugcua     1920

acgagagaaa acagagauaa agggaauaga acaccauacg aauauuuggg uggagcugaa     1980

aacucagaau cuuggcaaag auuuguaucc uucguagaga gcaauaaagc auauaggcua     2040

gccaaaagaa caagacuacu aagaaaagau uucggagcaa aagaaagcga agaauuuaga     2100

gaaagaaauu uaaaugauac acgauaugug ugcagauucu ucaaaaauua uguagagcag     2160

caccuaaaau uggcagaagg aagugaaaca aaaagaugug ucguaguauc aggacaacua     2220

acuaauuucc uucgagccag auggggcuua cuuaaaguac gcucagacag ugauagacac     2280

cacgccuuag augcugcagu cguagcggcu uguagucaua guaugguuca aagauugagu     2340

aacuauucua ggacacguga auuagaacag guaagagacg gauucguaga uauagaaacc     2400

ggggaaauag uaaacccagc aaugcaccaa caguuacgug agcauuuucc agauccaugg     2460

ccuuauuucc gacaugaauu agaagcuagg cuaaaaauag auagucccga auuacuacgu     2520

gaagaaaugg cuagacuagg aucauauagc gaagaagaac uaaaaguuuu aaaaccacua     2580

uuugucagca gggcuccuca aagaaggaau gggggugcag cucauaaaga cacaaucuac     2640

ggacaaccag aaaaauuaaa gaaaaaugga agcguaacuc aaaaaguagc gcuaacuuca     2700

uugacccuca aagauauuga ucuguuagca gauccacaca ggaaugcgaa acuuuacggc     2760

gcuauccgug aaagacuaga agcacaugga ggaaaaggcg auaaagcauu cccagcuaca     2820

aacccgcucc gaaaaccuga caaagaagga aaucccacag ggccgauagu cagaacagua     2880

acaaaaguaa uugauaaacu aagugggauu ccaguaagag gagguauagc uaaaaaugau     2940

acaaugcuaa gaguugacgu auucacaaaa ggagguaaau uccaucuugu accaguguau     3000

guccaccaca gaguaacagg auugccaaau agagcaauaa uagcauucaa agaugaagau     3060

gaauggacac uuaucgauga uaguuuugac ugguguuuca gccuauaucc aaaugaucug     3120

cuacguauaa aacuuaaaaa ggaaagucac ucagguuacu auucaucaug caauagguca     3180

accggagcug ugaaccuaug ggcucacgau aggaacguua auguagguaa ggauggauuc     3240

auagaaucaa uagguguaaa aauggcggug uccauagaaa aauuacaugu ggauacuuua     3300

gguaacaucu auccagcacc accagagaaa agaagaggac uagcauga                  3348


<210>  5090
<211>  3351
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5090
augucacaaa aucaaaaccc aaacacacaa ucacauuuaa acccuacaac acaauccagu       60

acccaaagua acccacaacc aacaacacaa gaaagucuaa aaaucuuggg auucgacaua      120

ggcguagcgu cuauagggug ggcauuuguu gaagguggag aauuaaaaga uugcggcguu      180

aggauuuuca caaaagccga gaauccuaaa gauggaagca gccuagcacu gccacggcgc      240

gaggcuagag gaguaagacg uagauuagca aggcgaaaag gaagacuaaa ugcauuaaaa      300

guacuaaucu guaaagaauu ugguuuaaau cgaucugauu aucuagcaac agcaggcgaa      360

cuaccgaaag cauacgccac aucuaaagaa acaaaaucuc cguaccaacu aagggcagaa      420

ucccuagaaa gaaaauuaag cgcugacgaa uuugcuagag uaauauuaca uaucgccaaa      480

cauagagguu auggaaacaa acaugcuaaa gagucaagcg augaugacuc aggaaaagua      540

aagaaagcaa uuucagaaaa ucaaaaaguc auguccgaaa aagguuacac cacagcgggc      600

caauauuuau auggugaguu cuaccaaaaa gcuagaaacu ucagugaagc cgaaccaaaa      660

agcccgaaaa acgcuagggg aacccaagag uucaaaaaug uacgaaacaa aagugaaaac      720

uacgccagau guuuagcuca aagugaauug caaaaagaac uagaaauaau auucgcaaaa      780

caacgcgaau uuggauuuca auuuagugac aagaaacaca aaacuauaaa cgcaaacgga      840

aaauugaaag aacuagacuu cgcagaugca guuuuagaaa uagcauuuuu acaaagaccg      900

cucaaaucau uugccgauaa agucggaaaa ugcacuuucu augaagacaa accccgagca      960

ccuaaagaca gucuaucggc uauagaauuu gucgcacuaa ccagaauuau aaauacauua     1020

gcuaacauaa cuaaacgaag uggagaagua uauagugcag auaaagugag agaaauacuu     1080

agcauagugc ucgauaaagg ugaaauuaca uacaaaaauc ucaggaaagu aauagcccua     1140

gaugaaagau uacaauuccc caaagacagc aaauuagauu augcaaagga agaugccgaa     1200

aaagcuaaag ccauagaguu cucaaaauua aaagccuuca gaaaagcccu cggagggucg     1260

uucgaaggau uuauuagggc agaauuagac agcauagcaa cagauaucgc auuaauuaaa     1320

uccaaagacg aauuaaaagc uaaauuagca aauuaucaau cguuaaauga ugaacaaagg     1380

gaaaagcuaa gcgaacuuag cuuugauaaa uuuauagacc ugaguuuuaa agccuuagaa     1440

gcaauacucc ccuuaaugag gggugauaag gaugauaaaa uauacagaua ugacgaagca     1500

uggagagaag cuggauuagu ugaaguggcc aagaaaaccc aaaaagggga uucauugcca     1560

ccgcuaauag aauacgaaaa agacuuggca aauccaguag uagcaagagc acuagcagaa     1620

uacagaaaag uacuuaaugc cuuacuaaag aaacauggaa aaguucacaa aauucaucuu     1680

gaguucacca ggcaggcagg aguaucuucu aaagaaagag gagaaauaau cgcggaacaa     1740

aacaaacgau uaaaagcaaa ugaagaagca agaaaaagau gcgaagaaua cggacucauc     1800

ccaucaggua aaaacauacu aaaacuaaaa cuauggcaag aacaaggcga auuuugcgua     1860

uacagcggua agaaaauaac ccaaagugac cucagagaug aaucaacauu acaaauagac     1920

cacauauacc cauacaguag gaguuaugau gauucgcaaa acaacaaagu gcuaguauuc     1980

acugaugcaa aucaaaacaa acucaaucaa acaccuuacg aggcuuuugg uaaagauaua     2040

ucaaaaugga accaaaucuu agggagaaua gaaaaauuac caaaagcaaa gaaaaauaga     2100

aucacaaaua aaaauuuugc aaacaaagaa gcgggcuuua uugccagaaa uauuguagau     2160

acaaguuaca uugcuaggcu uagcgcaagu uauaccgacg cauuuauuga guuuugcccc     2220

cucgcagaaa augaagacac uacccuagga agaggcgaaa aagguuccaa gaaacauauc     2280

agcgugguca augguucauu aacagcgacc augagacacu acuggggccu aaacacauua     2340

uuggacggag aaaauaguga aucaaaagau cgaaguaauc acuuacacca cgcucuagac     2400

gcuauuauca uagcauacgu gaacgauuca guaaucaaag ccuuuagcga uuucaagaaa     2460

acacaggaac aaaauaaagc aaggcucaua gcaaaagaaa uugaaagugc ugaauauaaa     2520

aucucacgac gauucuuuuc accaaguggg uucgaaaaca acgaggcauu uagacaagca     2580

auaaaaagaa aaauucuagg acaaagucua gagucacgag agaacgacau gacaauuaac     2640

ucggaggcau uaggcgagaa aguaggagga auauuuguuu caaaaccacc aagaaaacgu     2700

gcuagaggcg cucuacacaa agagacauuu aguucaauag acgacaaaga ucuacuuaaa     2760

acauauggag gaaaagaggg cgugaaccgc gcuauaaaau uaggaaaagu aagacaaaua     2820

gggucgaaaa uagcugacaa cggaacaaug guuagaguag auauuuucag acacaaaguc     2880

ucgggcaaau ucuacggagu accaaucuac acaauggauu uugcacuugg cauucuacca     2940

aacaaagcag uaguaggagg aaaagauaaa ucagguguaa ucaaagacug gcucccuaug     3000

gacucaaacu acgaauuuug cuucucauug uuuaaagacg aucuuauccu cguccaaaag     3060

aaagaaaugu ccaaagcaga acuauguuau uucgcugguu ucgauacaag uggaggacaa     3120

auuaaaguag aaaaacauga uaaccauuuu gcuacacuaa cugauaacca aaggcuacua     3180

uucaauauag caacaccaaa agaaguugca ggaaaaagua ucggaauaca aaacuuaaaa     3240

guauuugaaa aauggcaagu cucaccacua ggcgaaguuu ccaaagcuga auuuaaagaa     3300

agagaaccua ucgcacuaaa aaguacccca aaaagauuuu uacaaggaug a              3351


<210>  5091
<211>  3354
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5091
augaaacaca uauuaggacu ugauuuagga agcaacucaa uuggaugggc auucguacaa       60

caagauuuug aaaauaaaca agguaaaaua auagcaacug gaucacguau aauaccaaug      120

gaucaaggua uucuagguga cuucgaacga ggcaauacag uaagccaaac agccgaacga      180

accacauaua gaaguaugag gcgacuuaga gaaagacacc ucuuacgccg agaaagacua      240

cauagaguac uucaccuauu aggcuuucuc ccaccacacu augacgccca aaucgauuuc      300

acaaaaagau acggaaaauu uauagauaau gccgaaccaa aaaucgcaua uaauaaugga      360

aauuucauau uuaugaauuc cuuuaacgaa augguagaag acuuuaaaaa acaccagccg      420

cagcuauuuu auaaaaaauc aaacggcgaa gaaucaagaa uaccuuacga cuggacuaua      480

uauuauuuaa gaaaaaaagc acuaagucaa aaaauaacac aacaagaauu agcauggcua      540

auacuccacu uuaaccaaaa acgcggauau uaucaacuac gaggagagga agaaacagaa      600

aacccgaaua aagaaguagc uuuucauagu cuuaaaguag uagaugucga agccgaagca      660

ccaaauaaaa aaggagaaau augguauaca auaagauuag aaaacgguug gaucuauaga      720

cggacaagca aaaauccauu agaugauugg aagggaaaaa caagagauuu cauaguaaca      780

acagaccuca acgaugaugg uacaauaaaa uuagacaaag aaggaaaaga aaaaagaagc      840

uucagggcac cagaugaaaa cgacuggaac cuacuaaaaa aaaaaaccga acaugacauc      900

gacuucucag gcaaaccagu cggaacauac auuuaugauc accuuuuaca gaacccgaau      960

cagaaaauca aaggaaaauu aguccgaaca auugaaagaa aauacuauaa agaugaacuu     1020

aaagcaauac uugccaaaca aauagcgcuc caaccagaau uauucacaga aacccucuua     1080

gcugacugca uucgagaacu auacagaaaa aacgagaccc aacaacaaaa uuuacucagc     1140

aaagacuuuu ugcaucuauu uguagaagau aucauauuuu accaacgucc acuuugcagc     1200

aaaaagucaa cuauugcaaa cuguacacug gaaaaacgga guuacauaga uaaagaaacc     1260

aacgaaagaa aagacgcacc aauaaaagua ugcgcuaaau caaauccuua cuaccaagaa     1320

uuccguguac uucaaugguu acaaaaucug agaauauaug aaaucgaaac agacagagaa     1380

guaacagcag aguuuaucga gacaccucaa gacuacgaag cccuauucaa cuucuuaaug     1440

ucucaaaaag aaaucgauaa ugaaggauua uuaaaguauu uucuauucgc aaagaaccca     1500

caaauuaaag auaaagccgc aaaaagugaa uuaaaaaaau ggcuuacaac cuauagaugg     1560

aacuaugugu acgaugcagc ugauaacucc ucgaaaaaau accccaugaa cgaaacugga     1620

uaugaacuuc aacgauacuu acaaaaagua gaaggccucc ccgcaaacuu cuuaacuuuu     1680

gacaucacuu aucaacucug gcaucuacua uacucuguaa aagacaaagu agaauucgaa     1740

aaagcucuua aaaaguucgc uaaaaaaaaa caauuggaua aaacaucuuu cgucgacagc     1800

uuuaaaaauu ucaaaccuua ucccaaugaa uacggaucuu ucucagaaaa ggcgauaaaa     1860

aaacucuugc cucucaugcg auuuggaaau cacugggacu uuaaucgaau agauguaagu     1920

acucaaaaga gaauagacga ccuaauuaac ggcguagcaa acgaagauau ucgaacgauc     1980

auacgcgaaa aagccgaaaa auaccaauua aauucugaaa ccuguuucca agacuuacca     2040

cucuggcuag cacaauacgu agucuacaac cgacaugccg aagcuaccga cauagaaaaa     2100

uggacaucug ucucagaccu ugaagcauac cuggaagauu ucaaacaaca uagucuuaga     2160

aacccgauag uugaacaagu uguaacagaa acacuacggg ucguucguga uauauggcaa     2220

caguauucgg gaggucaagc aaauuuuuuu gacgaaauac acaucgaacu uggacgagag     2280

cugaagaaga cagcagaaga acggaaacaa uugucagaca agaaucaaga aaacgaaaac     2340

acaaaccuaa ggauaaaaaa guugcuggcc gaacuaaaag aagaugcauc uauccaaaac     2400

guaaggccau acucauuggu gcaacaggaa cuauugaaaa uauacgaaga aggaguauuu     2460

ucauccacgg augaaauaga agaagacauu cagaaaauaa gaaagaaagu cgaaccuaca     2520

ccacccgaaa uacaacgaua uaaauuaugg uuagaacaaa aauauaaaag uccauauaca     2580

ggagaaguaa uaagccuaac uaaacuauuc acugaagaau acgaaauuga acacauaaua     2640

ccacagucac gauacuauga ugacagucuc uccaacaaag uaauauguga aagcgcuguc     2700

aauaaauuaa aaacgaacca gcuaggauua gaauuuauac aaaaccacca cggagaaaaa     2760

guacgaauaa cagcuaacaa agaggucacc auauucacag aagagcaaua caaagaauuc     2820

guaaaaaagc acuaugcgaa caauucugcu aaacaacaaa aacuacuccu aaacgaaauu     2880

ccagaaaaga ugguugcgag acaacuaaau gacacacgau acaucuccaa augcauuucc     2940

gaaaucuuau cuaaaaucgu acguagcgau gaaaaagaug aaggaguuaa caguaagaac     3000

aucauaccuu guacaggaaa aauuacuacu gcacuuaagc aagauugggg acuaaaugau     3060

guauggaacg aacuaauacu accgagguuu gaacgaauga aucaacuuac acaaacacaa     3120

uuguuuacaa guuacaauga caggcuucaa aaaaaccucc ccacuguacc aauugaauac     3180

agccaaggau uucaaaaaaa aagaauugau cacagacauc augccaugga cgcauuaguc     3240

aucgcaugcg caacaagaga acacauaaac uacaugaaua aucaacaugc ccucgacaaa     3300

aguaaagaca aaaaagaaaa gcaacaaagu cgaaucgacc uccauccuuu auga           3354


<210>  5092
<211>  3354
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5092
augcaaacua aaaauuuguu guacacauua ggguuagaua uagguauagc aagugucggu       60

ugguguguau uaggagaaaa uaggauuauc gacuugggag uaagauguuu ugacaaagcc      120

gaaacaccag acagaggaga aucauuaaau aaaguuagaa gagacgcaag gcuaaugcgu      180

cgaagacuaa ggagacgagc cuggagacua acaaaauuag ccagacuacu aaaacacgca      240

ggccuuauug auuccacucg aauaguaaga gaucggcaug auggggccgu cucuccaugg      300

caauuaagag uugaaggacu ugaccgaaaa uuagcaccag aagaaugggc aagagucauc      360

uaccauauag ucaaacauag aggauuucac uggauuucaa gagcugagga aagaaaggca      420

gagucagaua aaggagaagg gggaaaagua aaacaaggac uaagcgcaac uaaacaacuc      480

augguagaga aaaauuacag guccgcagcu gaaaugguac uaacagaguu cccaggagca      540

caaagaaaua aaagagggga auauacuaaa gcucucagua gaauacuacu ugcagcugaa      600

cuuaccacau uguucgaaag acaacaagag cuuggcaacc cgcaugcagg accagacuua      660

caaaccaaaa uauuaggcaa uggagauaga aaaagugguc uuuuauggau gcaaaagccc      720

ccgcucagcg gcucugcauu gcuuaaaaug cuuggaaagu guacuuucga acgagaagaa      780

uauagagcac ccaaagcauc uuuuacggcu gaaagacacg uuuggcuaac aaaauuaaau      840

aauuuacgag uauuaguaga cggcguaacg agaccauuau cagauccuga gaggaggcuc      900

gcguuaaguu uaccauauca gcaagcaagu gacuuuacau auaaacaacu aagagcagcu      960

cuaugcaaaa augggcuacu cccagauucu uuucaauuuu cgggacuggc uuacccaaca     1020

ggugcccaac agccagaugg caaagcuaaa aauccagaga acgaccgauu agucaaacua     1080

ccggcauggc aggaguuccg aaaaacgcua agagacgcag gacuagaaac agaaugggaa     1140

ucaauagcgg gaauggcagc cggugggcaa ccagauuuau uagaucaaau aggcugggua     1200

cuuuccguuu uuaaagaugg agacgaagua cuacgugaac uaagaaaaau accacuacca     1260

ggaggagaga aaaugguaca ggcccugagu ggagugucau ucgauacuuu ucacaaucuc     1320

agucugaaag cauuacgaaa gauuguucca cauauggaag caggauuaag guaugaugaa     1380

gcaugucucg cagccggaua ucaccacagc gaucuaagaa aagauaccga ugaaagaagu     1440

uuauacuuac caccccuaua uucuggacgu acaacagaug gaaggauggu auucagagau     1500

gauauagaug uaccgagaaa uccgguugua uugcgcgcga uaaaucaagc aaggacuguu     1560

guuaauagug uaguacguag auaugggucu ccaaguucca ugcauaucga aauggcucga     1620

gacuuaaguc guccauggga cgaacgaaac aaaguaaaaa gagcacagga agaauauaga     1680

gacagaaaug aaaaacuuaa aauagacuuc gcagcagagu acgguauaga accaaaaagc     1740

agagaauuug aaaaauucuu acuauaccgu gaacaaggag gaaaaugugc auacagucug     1800

aaaccacucg aucuugacag agucuuaaga gaucucggau augcagaagu ggaucaugca     1860

cugccauaca gcagaaguuu ugaugauagu aaaaauaaua aagucuuagu ucuggcggaa     1920

gaaaauagaa acaaaggaaa uaggacacca uaugaauauu uggauggaac cucagaaagc     1980

ccaagaugga gagaauuuaa agcauuuguu gaaacaaaua aagcauaccg acuagcaaaa     2040

agagcucguu uauuacgaaa agauuuuuca ggaaaaggaa gcgaagaauu ucgugaucga     2100

aaccuuaaug acaccagaua uauaucaagg uucuuuaaaa acuauguaga aagauuucuu     2160

aaguuagcag aggaauccga agaaagaacc uguguagugc uaagcggaca auuaacagcc     2220

uuucuaagag cgagaugggg auuagcaaaa guaagaggcg acucagauag acaucaugcc     2280

uuggaugcgg caguuauugc agcuuguuca agaagcaugg ugaaacgacu aucugacuau     2340

gcccgacguc augaauugga aaaggucagg acaggauucg uugacuucga aacaggugaa     2400

auaguggaug cggggaaaua ccgacaaaua gaaagagacu uccccagccc cuggccauac     2460

uuucgggaug aauuauuaac aagauugaau acagacgacc cagccgaacu uagagaaaga     2520

augcaaucuu ugggauccua uccaccagaa gcacuugaaa cacuuagacc aauguuugug     2580

ucaagagcac cucaacgacg aaacggagga gcagcacaca aagauaccau auauagacaa     2640

acagaacgau uaaagaaagu uggcucggua acacaaaaag uuccauuagc agcacuaaau     2700

cuuagagacu uagagaaucu cguagaccca cauaggaaug aaaaacuuua cgcagcaaua     2760

cgagcacgac uugaagccca uggaggaaaa ggagagaagg cuuuuccccc aaauaauccu     2820

cucagaaaac cugauagaga cggaaauccu acgggaccaa uaguuagaac uguaacguua     2880

guaauagaca aaaugucagg cauuccaguc agagguggag uagcgaaaaa ugacacuaug     2940

uuaagagugg auaucuuucg aaguaagaaa gacggcaaau uucaccuagu accauuguau     3000

guacaccaug caguugcaaa acaauuaccu acaagagcag uaaucgcaua uaaagaugaa     3060

ggagaaugga cuccgauaaa uaaucaugau uuugauuuuc uguuugcauu auacccuaau     3120

gauuuaguac gaauaacccu aaagaaagaa agacacuuug gauacuaugc agggugcaau     3180

agagcaacag gagcaguuag cauaugggca caugacagaa accaacgaau uggaaaagac     3240

ggauuuauag auggaaucgg aguuaaaaca gcauuaagug uugaaaaauu ucauguggac     3300

acacuaggaa acguauaccc agcaccuccu gaaacucgaa gaggccucgc uuga           3354


<210>  5093
<211>  3357
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5093
augggaacga gauauucauu ugauauagga acaaauucaa ucggaugggc cguguggaga       60

acaggaagag auccaaaagg uauauucgga gagaaagacg caccucuuga auuauugggg      120

gcagggguua ggcucuuuaa agacgggaga aacccuaaag acggucaauc acuagcgacu      180

augcgaagga uaccacggca agcucgaaaa cgaagagaua gauuuguauu aagacgugaa      240

gcucuaguag cugcauuaau ugaagcagga cugaugccug cuucugaaaa agaacgaaaa      300

gcauuagcug auuuagaccc guaccaacug agagccgcag gauuggacaa agcauuagcc      360

ccacaugaac ucgguagagc aauuuuccac cuacaucaga ggagaggauu caaaagcaac      420

agaaaaacag auaggaaaag ugaugauaga gauaaaggga aaauagcuac ggcuagcgcu      480

gcacuagcua aaaaguuuga agaugaaaau gccagaacau uuggugaauu ucuauggaga      540

cgucauagag acccagauau gaaaagacaa gcaguauuaa aagcacauga uccugcuaaa      600

ggcaaaccag acuuaagggc ggcuaaauua agacaaccaa cgagaauuag auuagaaggg      660

caaggagcca ccgcacuaua cgaauuuuau ccaacuagag aaaugcuaug ucacgaguuc      720

gaugcacuuu gggaagcaca agcccgacac cacccaaugu uacuaacaag ggaagcagga      780

gauaaaauua gauccauacu uuuccgacaa agagaacuaa aaccaccgcu aauaggcuua      840

uguacauucg aauaugcggc uggagaaaaa gaaucaaggu uaccuaaggc cuuaccguca      900

guacaagcga gagaaauaua ugaaaggcua ggucauauaa gauuaucaac aggaucgggg      960

acagacagac cauuaaaauc aucagaacga gacgauuugg cuacugcuuu guuacauugu     1020

ggguuaacaa aaauaaaagu uaguaaaacg aaaggauuag cguuuaaaac cgaauuuaga     1080

aaagcgcuaa ggcuuggagg ugaagugaga aucaacuuug aagaaauggg agaagaagga     1140

uuaguagacu uaagaacaaa cagauuauua guccaagacg aucauuacgg uccaagaugg     1200

agggcccuaa gcuggagugg aaaagaugca uucgucaaga aacuauugga agcaacagau     1260

gacgacaaau uaguggcacg uuuaaugaca gaagauggau uaucagcugu ccaagcgaga     1320

aaugcagcgu caauacccuu accagaugga uacaguaggc uugguccgac agcuaaugca     1380

gcaauauuac aagcacugau cgacagcagg gaugagaaug gauucguagu aacauacgac     1440

aaagcagugc gacgcgcugg ugaaaggcuc ggaaaagacu ggcaucauag ugaugaacga     1500

gauggugaaa uauuagacgc acuuccauau uacggaaaag uacuucaacg acauauacuu     1560

ccugguauga uggauccggc ugauagaaaa gacgaagcaa aauuuuaugg uaggaucaug     1620

aauccuagcg uccauauugg guuaaaucaa cuuagacguc uagugaacgc ccuuauagaa     1680

agguucgggc ccccugauca gauaguagua gaacuggcaa gggauuuaaa aaugucugca     1740

agagaaaaag aacaagaaca aagacgaaau agagauaaca gagaggcaaa ugagaaaaga     1800

acgaagaaau uaguagaauu ggggcaagau aauaacggag aaaacagagc ucggcugaaa     1860

cucuaugaug aacaacaaag ggcagggaac ggagucgcuc auugcccauu uacguuagac     1920

cccauuggga uaacucaguu guuuucuccu gaaauagaaa uagaacaugu cuuaccgaga     1980

aguagaaccc uagaugauag ugcagcgaau aaaguacuau guuuuagaac aauuaauaga     2040

auuaagagag gaaaaacgcc augggaagcg uuucacgcag auccuaaaug gcccgauauu     2100

gcagcaaaug cugaaaaacu gcccccaaau aaaagaugga gauuuagaga agacgcuaug     2160

gauguguuug aggcaagaac aagugguaaa auggauaaga caacacugga gcaaauggga     2220

uuaacaacag guuuucaagc aagacauuua aacgaaacga aacacauguc cagauuggcc     2280

aaggccuauu uaggaaaaau augcgaucca gacgcaguuu augucacacc aggagcauua     2340

acaaguuuac ugcgaggcaa augggguuua aacucaauac uaucagauga uaauuuaaaa     2400

gacaggacug aucacagaca ucaugcaaua gacgcaauua cgauaggagc gaugacaaga     2460

ucauuauugc uugaauuaaa cacauuagcu gguaaagcag aacagagcga uuacgaugau     2520

guagucggga agguaccaug gccauucgac aacuuccgag acgcaguucg guccguugua     2580

gauagaguug uaguaucaaa uaaaccagaa cacggaaaac agggagcuuu acaugaggau     2640

acagcguaug ggauagucgg ggacgaagca gaggcagaag agauaggaaa ucucguuaga     2700

agaaaauugc uuguugaccu aacaccaggu gaaguagacc ggauucgaga ugcgggacua     2760

aggaaagcaa ugcaagcagc aguugcaccc agcagagaug cuagaggaaa agucucugau     2820

gcuaagggau ucgcagcagc auuagacacu uuugcaaaag cacaagguau gagacgaauc     2880

agaguuggaa aagaagacau gucagcagua acgauccaug auagacaaac agauaaagca     2940

uauaaagcag uagcaccagg cgaaaaucac cacguagaug ucgugcagau gagagacggu     3000

gcauggaaag guuucgcugu aacgguauuu gaagcaaacc agaaggauuu uagaccacaa     3060

ugggaaagag agaaguuagg agguaaauua guaaugagau uacauaaagg ugaugcagua     3120

gaacuagaau caucuggagu uagacuggua aaauuuguac agcagauaug gaugaaauca     3180

aauuuaguac ucuuaacaga acauaaugaa ggaggggacc uacaaaaacg acaccaggau     3240

gcagaugacc cguuccgaug ggauuucgca aauauuggaa aauuaaaaga aagaggaugu     3300

gucgcuguua aaguggauga auuagguagg guaacaaaaa gaauuucaaa uacauga        3357


<210>  5094
<211>  3360
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5094
augggauaca gaauaggauu ggacguagga auuacaucaa caggcuacgc gguauuaaaa       60

acugauaaaa acggccuacc auacaaaaua cucacgcuug auucaguaau auacccgcga      120

gcagaaaacc cacagacagg cgcuucacua gcagaaccaa gaagaauaaa aagaggacua      180

agaagacgca caagaagaac aaaauuuaga aaacaaagaa cgcaacaauu auucauacac      240

agcggacuac uaucaaaacc cgaaauugag caaauauuag caacuccaca agccaaauac      300

ucgguauaug aacuaagagu agccggucua gauagacgau uaacgaacuc ggaguuauuu      360

agaguguuau auuucuucau aggccacaga ggauucaaaa gcaauagaaa agccgaacua      420

aacccagaaa augaagcaga uaagaaacaa augggccaac uccuaaauuc cauagaagaa      480

auaagaaaag ccauagcaga aaaaggauac agaacagucg gcgagcugua ucuuaaagau      540

ccuaaauaca acgaccauaa acguaauaaa gguuauauug acggauauuu aucuacuccc      600

aaccgacaaa uguuaguuga ugaaauaaaa caaaucuuag auaaacaaag ggaguuaggg      660

aacgaaaaau uaaccgacga auuuuacgca acauauuugu uaggagacga aaauagagcu      720

ggaauuuuuc aggcacaaag agauuucgac gaagguccgg gggcaggucc uuacgcagga      780

gaucaaauca agaaaauggu uggaaaagac auauucgaac caacagaaga uagagcagcg      840

aaagcgacuu auacauucca auacuucaac cuccuucaga aaaugacaag ucugaacuac      900

caaaacacaa caggagauac uuggcauaca cuaaauggcc uggauagaca agccaucauc      960

gaugcaguuu ucgcgaaagc agaaaaaccg acaaaaacuu acaaaccaac ugacuucgga     1020

gaguuacgaa aauuauuaaa auuaccggau gaugcuagau ucaaccuagu caacuauggc     1080

agcuuacaaa cgcaaaaaga aauagaaacc gucgaaaaga aaacaagauu uguugauuuu     1140

aaagcauauc augaccuagu uaaagucuua ccugaagaaa uguggcaauc acggcaacuc     1200

cuagaucaca uaggaacagc acuaacacuu uauucaucag auaaaagaag gagaagauac     1260

uuugccgagg aauuaaacuu accagcugaa cugaucgaaa aacuauuacc acuuaauuuc     1320

aguaaauuug gacaucuauc caucaaaagc augcaaaaua uuauuccaua ucuagaaaug     1380

ggacaagugu auucagaagc uaccacuaac acuggauacg acuuucggaa gaaacaaaua     1440

uccaaagaua caaucagaga agaaaucaca aauccagucg uaaggagagc ggucacuaaa     1500

acaauaaaaa uaguagaaca aauaauaaga agauacggaa aaccugacgg uauuaacaua     1560

gaacuagcaa gagaacuagg aagaaauuuu aaagaacgug gugauaucca gaaaaggcaa     1620

gacaaaaauc gacaaacuaa ugauaaaaua gcagccgaau uaacagaacu aggaauacca     1680

gucaacggcc aaaauauaau acgauacaag cugcacaaag aacaaaacgg agucgaccca     1740

uacacgggag aucaaauacc cuuugaacga gcauucucag aaggcuacga aguagaccau     1800

auaauaccau acucaaucuc augggacgau agcuauacaa acaaaguauu gaccagugca     1860

aaauguaaua gagagaaagg caacagaaua ccuaugguau aucuugccaa uaacgaacaa     1920

cgauuaaaug cacuaacaaa uauagcagac aacauaauuc gcaauucacg aaaaagacaa     1980

aagcuacuaa aacaaaaacu aagugaugaa gaauuaaaag auuggaaaca aagaaacaua     2040

aacgauacga gauuuauaac gcgaguacua uacaacuacu uuagacaagc aauagaguuc     2100

aacccagaau uagaaaagaa acagagaguu cucccucuga acggugaagu aacaucaaaa     2160

auuagaucuc gauggggauu ccuuaaagua cgagaggacg gagacuuaca ccaugccauc     2220

gacgcaaccg uaauagccgc uauaacaccu aaauuuauac aacaaguuac caaauauagu     2280

caacaucaag aaguuaaaaa caaccaagcu cuauggcacg augcagaaau uaaagacgcu     2340

gaauacgcug cagaggccca aagaauggau gcagaucuuu ucaauaaaau auuuaaugga     2400

uucccuuuac cuuggccaga auuuuuagau gaacuauuag cuagaauaag cgacaacccu     2460

gucgaaauga ugaaauccag auccuggaac acuuauaccc caauugaaau ugcaaaauua     2520

aaaccaguau uuguaguccg acucgcaaac cauaaaauca gcggcccagc acaucuagac     2580

acaauacgaa gugcaaaauu auuugaugag aaaggcauag uacucucaag aguuucaauu     2640

accaaacuga aaauaaacaa gaaaggccaa guagcaacug gagacggcau cuacgaucca     2700

gaaaacagca acaaugguga caaaguagua uacuccgcaa uacgucaagc guuagaagcg     2760

cauaacggaa gcggcgaacu agccuuucca gauggauauc uggaauacgu agaucauggg     2820

accaaaaagc uagucagaaa agucagagua gcuaagaaag uaucccuacc aguaagauug     2880

aaaaacaaag cagcagcaga caauggauca augguacgca uagacguauu caacacaggc     2940

aagaaauucg ucuuuguacc uauauauauc aaagauacag uagagcaagu ucuacccaau     3000

aaagcaauag cuagggggaa aucacuaugg uaucaaauua cagaaucuga ucaauucugc     3060

uucucuuuau aucccggaga uaugguacac aucgaaagua aaacuggcau aaaaccaaaa     3120

uacucaaaca aagagaacaa uacuucagua gucccaauca aaaacuucua uggauacuuu     3180

gacggagcag acauagcaac agccaguauu cuuguaagag cacaugacuc aucauacacc     3240

gcacguagua uaggaauagc aggucuuuua aaauuugaaa aauaccaagu agauuauuuc     3300

gguagauacc acaaaguaca cgaaaagaaa agacaacuau uuguuaaaag ggaugaauga     3360


<210>  5095
<211>  3360
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5095
augaaugaca agaaaaaucu aggaccguug acauucggau uugacauagg aauagcuagu       60

guagguuggg cagugcuauc uccuacaaga aucgucgauu ugggaguuag gugcuuugau      120

aaagcugaaa cagcaaaaga aggagaaagu cuaaaucucg cucgaagaag ugcuagacua      180

augagaagac gguuacgcag acgagcuugg agauuaauaa aacucgcaag acuauuaaaa      240

caugaagggc uaauugcaga cacaaaaacu uucauacaac caucagcuuc acaugcuuca      300

ccauggcaac uuaggaaaga cggauuagac agagcacuuu cuccagaaga augggccaga      360

gugcuauacc aucuaugcaa acauagaggc uuccacuggg uuagcagggc agaagcaagg      420

aaagcagacg gagacacuaa aggagaagga ggaaaaguaa aacaagggcu agcaaguacc      480

acaaaauuaa ugaacgauaa aucguaucga acagcagcag aaaugguucu cgcugaauuu      540

ccaucagccc agagaaacaa acggggagag uauacuaaag cauuaagcag agaauuauua      600

gacaaugagu uaaaacaacu uuuugaaaga caaagggagu acggcaaucc acaugcggau      660

acaaacuuag aggcaaaaau aagaggaucc ggagaccaua aaucaggauu auucugggaa      720

caaaaacccg cuuuaucagg aaacgauuua cuuaaaaugc uugguaaaug uacauuugaa      780

aaacaggaau auagagcacc caaagcauca uuuacagcag aaagacaugu guggcuaaca      840

cgguuaaaua acuugcgaau uauaguugau gggagaacca gaccguuagc ggaugccgaa      900

agaacuaugg cgcuaagucu accauaugaa caaguaggcg acuuuacgua uaagcaacuc      960

agggcugcau uagucaaggc aggauuaaug ccugauaguu ucaaauuugc aucccuagca     1020

uauccaagcg aauuucagaa ggcagaagga aaagcaaaag auccugaauc agcugccuua     1080

acuaaacuag cagcguggca ggaacuaaga gcuacgcuua agaaagcaga uuuagaaucu     1140

gaauggcaac aaauaaguug ugccgcucuu gaaggucaac cugaaacuuu agaugaaauu     1200

gcaggaguau ugucaguuuu caaagaugau gaugaaguuu ugaaugaauu gcaaaaacuc     1260

ggacucccag gaggagcaaa aacugaaaau gcuuuacucg acaucagauu ugacaaauuu     1320

caugcauuau cauuaaaggc uuugagaaaa aucguacccu auauggaaag aggucuacga     1380

uaugaugagg cuugugaaag agcagaauau caucauagcc aacuacauaa aguaggugag     1440

gguaaucgaa aauacuuacc acccuuauac aaaucccgug acaaagaugg aagaaugguc     1500

uuuaaugacg aacucggcga uauaccccgg aauccuguag uacugcgauc cuuaaaucaa     1560

gcaagaaaag uauuaaacgc ucuaaucgca agauacggau caccgaguuc cguccauaua     1620

gaaauggcaa gagaacuauc aaaaccauuu gaugagagga ugaaaguugg caaagaacaa     1680

gaggcuuaua gagaaagaaa cgaaaaagau aaacaaacuu uuguagcaga guucgguaua     1740

gucggcgcag ccaaaggggg ugaauuugaa aaauggaaac uguaucguga acaacaagga     1800

aaaugugcau auaguauaca aggaauagac cuuaauagau uguuggaacc aggauacguu     1860

gaaauagacc augcuuuacc uuacucacga aguuucgaug auaguaaauc caauaaagua     1920

uuaguuuugg cagcagaaaa cagaaauaaa ggcaauagga caccuuacga auacuuagau     1980

ggagaacaua auucagaacg auggaggcaa uuugucggcu uugucgaguc aaauaaacua     2040

uauagacaag caaagagaag uagauuacua agaaaagauu uuggcaaaga acaagcagau     2100

gaauuuagag aaagaaaucu uaacgauaca agauauguuu gcagguuuuu caagaaucaa     2160

guugaacaau uuuugaaacu ugcggaagga ucagaaucaa aaagaugugu aguacuuucu     2220

ggacaacuua ccgcauucuu aagggcaaga uggggacuaa uuaaagugag ggaugaaagu     2280

gaccgacauc acgcauuaga ugcagucguu guagcagcau gcacacaugg uaugguaaaa     2340

agaaugagcg auuacgcaag aagaaaagag cuugaucaag uagccgaggg cuuuguagac     2400

guagaaacag gugaaauaau uaaugcagca auguuucaac aacuccacga gcauuucccu     2460

gacccguggc cccacuuccg agacgaaguc caaaugagau uaaaaaucga cgacccagca     2520

caauuaagau cugauuugcu acgucuagga aauuacgcug aggaggaugu acaugcaguc     2580

aggccauuau uuguuucacg agccccgcaa cguagaaaua gcggugcugc ccauaaggaa     2640

accauauaug gccaaccuga aagauuaaaa gcacaagggg gagugacgga aaaaguggca     2700

uuaacagcac uaacaaugaa agaucuugau cguuuaauag auccccaucg gaaugaaaaa     2760

uuauacgcag ccauacgaaa acggcuugaa gaguucggag gaaaagcaga uaaggcauuc     2820

gcagcaucaa auccacuaca caaaccggau aaagaaggaa auccuacugg accgaucgua     2880

agaacuguaa cacuagaaau agacaaacuu ucaggaguuc caguaagagg gggaauagcu     2940

aaaaaugaua ccaugcuaag aguagauguu uucaccaaag caggaaaauu ucauuuagua     3000

ccaguauacg uacaucauaa gguuacagga cuaccuaacc gagcaauugu agcaagcaaa     3060

gaugaagacg aauggacggc uauagaugac ucauuugaau uuuuauucuc acuauaccca     3120

aaugacuuag uaacgaucac ccagaaagga aaagcaccag uacguggcua uuaugcuagc     3180

ugccauagag gaacuggugc uuuuucacuu ugggcucacg auagagcaag uagugucggg     3240

aagggaggac aaauagaagg aauaggaaua aaaacagcau uagcugucca aaaauauaau     3300

guagacguac uagguacaac cuaugcagca cccuuagaac aacgaagagg auuagccuga     3360


<210>  5096
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5096
augaggaaag uaacaauagg auucgacaua gggguaucua gcgugggaug guccguaguu       60

gauaugaaca acggagcaau cguugaaacu ggugugagac uauuuucagc gucaucagcc      120

gaaaauaacg uagaaagaag aaauaaucgu caaaccagaa gacuaaaucg acguaggaaa      180

aacagauuaa aagauguuaa auaccuauua gaaacgaauc auuucacauu ugaucaagaa      240

caagguacaa auccuuacga auuaagggua gccggacuac acagaaaacu aacacgauca      300

gaacuagcaa gagcacuaua ccaccuagua aaaagaagag guauuagcua ugaccucaca      360

gacauagaag aagaagaaau agugacaacc agcgcauaca aaacagcauu gggcaaaaau      420

caaucacaau uggccgagaa aacaccaggg gaaauucaau uugccagacu cgaaaauuau      480

ggaaaaguac gcggaucuau agaaaucaac gaacaagaca uacuacuaaa cauauuucca      540

acaagcgcau acuuaaacga agcaaaaaga auauuagcua cacaaagaaa auucuacccc      600

gaaauaacag aagcauucau agaaaaauac ugcacaaucc ucacgagaaa aagagaauac      660

uauguagguc caggaacgga gaaaaacaga accgacuacg guaucuaccg aacuaaugga      720

gaaacccuug auaaucuauu ugaaauacua auagggaaag acaaauucua cccaaaugaa      780

gaaagagcug caggaaauag cuauacagca caacuauuca acuucgucaa ugaccuaaac      840

aaucugaaaa uacagacgau agaagaccaa aaacucacac ccaaacaaaa agaagaaauc      900

auagaacaag ugaaaaccgc cgagaagaaa guuucacuca uacaauuaau aaguaaaaua      960

acaaaaacac caaaagauca aauaacagga uacaggacag auaaaaaugg aaaaccugac     1020

cuacacucaa uggcaguauu uagaaaaguu agaaaacaau uuuuaacaau ggaaauagac     1080

aucuuacacu ggcccgauga acuucuuaac gauuuaggaa ggcuccuaac auuaaacaca     1140

gaaaaaggag aaaucagaaa acaacuaacg aaagacuuca ugaaacgcua cccuauacua     1200

aacgaacagc uugucgauca aauaauugaa aaccggcaca ucuuugccgu uagcgguaau     1260

cauaaauggc accgauucuc acuuaggaca augaacgaac uacuaccuga aaugcuagcc     1320

acaaguaaag aacaaaugac acugcuaucu gagagaggga ugauaaaaca acauaaacgu     1380

gacuaccuaa aaaugaauga auuagacgcc cgacugauau ccgaagaaau uuauaauccu     1440

guuguaggaa aaucaguacg agaagcaauc aaauuauuca acgaauuaau gaagaaauac     1500

caagaaguaa gguaugucgu acuggaaaug cccagagacg acaacgaaga aaaagccaag     1560

aaagaacucc agaaauauca aaaagaaaau gaaaacgaga aagaaauagc acuaucagaa     1620

uuucaaagaa aaggauucuu uagcgaugca caauuagaac gagcauuaau aaaagauaga     1680

aaacuaaaaa cuaaaauuag auuuuggcau caacaagaag guaaaugccu uuauucugga     1740

caaaucauaa caccggucga gcuauuaacc aacaguuucu cauuugacau cgaccauauu     1800

auaccacaaa gcguuaguuu cgaugacuca uuaaacaaca aaguauuaug cacaaaagaa     1860

augaacgauc aaaaaggaaa acaaacaccc uacggcuucu uacuaaaugg aaacggucaa     1920

ggauucgauc gcuuuaaagc aaugguaaac gccaaccguc uaaugucaaa agggaaaaag     1980

aaaaacuuau uauucaugga agaucuaaac aauauagaaa cacgaaaaag auucauugca     2040

aggaaucucg uagacacacg auacgcaucu agaguaguac uaaacgaaau acaacaauac     2100

uuaagagcca aaaacaaaga uacccagguu gcaguaguaa gaggaaaauu cacggcaacc     2160

cuucguaaac auuggaauau aaacaaaagu agagaaacuc accaccauca cgcccuagau     2220

gcuucuauaa uagcaauuac uccacuauua ucgguaugga aaaagaaaca aacacuaaua     2280

ccaaaaacuu uaucugaaga aaaacuagaa uuugaauaug acgauauagu ugaagauaca     2340

aucuucgaaa aagcacuuua ccaauuaccu aaagaaaacu ucaaagaaca auuaaauaac     2400

ucacagccaa acauaaaauu uucauaucaa gucgacaaga aaaugaacag aaaaguuagc     2460

gacgcgacaa uauauagcgu aaaacaagca aaauuaucga gcgacaaaca aagcgaagag     2520

uacguacuag caaaaauaaa agauaucuau agcguagaag gauauaaaac cuucgcaaaa     2580

auuuaugaaa aggacaaauc aaaauuccua auggcucaaa acgacccaaa aacauucgaa     2640

aaacuugaag aaauaauaca aucauaccca aguacuagag aaauaaucgu aggagaaaaa     2700

gugaaaguag ucgacguauc accauucgaa cuguaccgaa aggaauacgg auacaucacg     2760

aaauaugcaa agaaaaauaa cggaccgaua auccgacaac uuaaauauua ugaccacaaa     2820

auagguaugc auuuagauau aaccaaccag aaaaauaaug gcaaaggcaa gaaaguaaua     2880

uuacaacaac uaaaaucgug gaggacagac gucuauuaca aucacgaaaa acaggaauac     2940

gaaauaaugg guuuaaaaua uuccgaucua cguuuuaaga aagaccaaua cggcauaccu     3000

aaagaaaggu accaagaagu aaaacaaaug gagaaaauag caccacagag ugaauuuugu     3060

uuuucauuau accgaaauga uaggauaaaa gucuuaaaua aagagacaga ugaagaaguu     3120

gaacuucuau ucggagcaag aaacuacacg agugcaggau acgucgaccu gaaacccaua     3180

gacagaguag aauuuggcaa gaaagaaugg auuccaguau accaauaugc uacaagugac     3240

ggaaaaugca agaagaaauu ugcuaagaaa ggauacgaaa uuuauaaagu aaacacgacu     3300

auacuaggag aaaguuacua caucaagaaa gaaagaaugc uaccgaaaaa uauauuagau     3360

uga                                                                   3363


<210>  5097
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5097
augucagacc uaguacuagg auuagacaua ggaauaggaa gcguaggagu ugggauacua       60

aauaaaguaa caggagaaau uauccauaaa aauaguagaa uauuucccgc agcacaagcc      120

gaaaauaauc uaguuaggag aacuaaccga caaggacgac gacuuacaag aagaaagaaa      180

cauagaagag uucgacucaa cagauuauuu gaagagauug ggcuaauaac ggacuuuaca      240

aaaauaagua uuaauuugaa ccccuaucaa cuaaggguua agggccuaac agacgaacug      300

aguaaugaag aacuguucau cgcccuuaaa aacaugguaa aacacagagg aauaaguuac      360

cuagaugacg cuucagauga cggaaauuca ucagucggag auuacgcaca aaucguaaaa      420

gaaaauucca aacaacuuga aacaaaaaca ccaggacaaa uacaauuaga aagauaccaa      480

accuacggac aacuaagagg ugauuucacc guagaaaaag augggaagaa acacagauua      540

aucaacgugu uuccuacauc agcauacaga ucagaagcau ugagaauccu ucaaacucaa      600

caagaauuua acccacaaau uacggacgag uucauaaaca gauaccuaga aauauuaaca      660

ggaaaacgca aauauuauca cggacccggg aaugaaaaau caagaacuga cuacggaaga      720

uaccgcacau cgggcgaaac acucgauaac auauucggaa ucuugauagg uaagugcaca      780

uuuuacccag augaguucag agcagcaaaa gccucauaca ccgcccaaga auuuaaccug      840

cuaaaugacc uaaauaaccu uaccguacca acagaaacua agaaacugag uaaagaacaa      900

aagaaccaaa ucauuaacua cgugaaaaac gaaaaagcaa ugggaccagc aaaacuauuc      960

aaauauauug caaaauuacu cucaugugac guagcggaca uaaaaggaua uagaaucgac     1020

aaaaguggaa aagcagaaau acauacauuc gaagcauacc gaaaaaugaa aacacuagaa     1080

acacuagaca uagaacaaau ggauagagaa accuuagaca aacuagcgua cguacuaacu     1140

uuaaacacag aacgcgaagg uauucaagaa gcccuggaac acgaauuugc agauggaucu     1200

uuuagccaaa agcaaguaga ugaauuagua caauuucgua aagcaaacuc aucaauauuc     1260

ggaaaaggau ggcacaauuu cucaguaaaa cuaaugaugg aauuaauucc ugaauuauau     1320

gaaacuuccg aagaacaaau gaccauauua acaagacuug guaaacaaaa gacaacauca     1380

ucaucgaaca aaacaaaaua uauagacgaa aaauuacuaa cagaagaaau auacaaccca     1440

gugguagcca aaagcguaag acaagcaauu aaaaucguca acgccgcuau aaaagaauac     1500

ggagacuuug auaacauagu cauugaaaug gccagagaaa caaacgaaga cgaugaaaag     1560

aaagcaauuc agaaaaucca aaaagcaaac aaagaugaaa aagacgccgc aauguuaaaa     1620

gcagcaaacc aauauaacgg gaaagcagaa cucccgcacu cagucuucca cggacacaaa     1680

caacuugcua ccaaaauacg acucuggcac caacaaggcg aaagaugcuu auacacagga     1740

aaaaccauaa guauccauga cuugauaaac aaucccaacc aauucgaagu ugaccauauu     1800

cuaccacuaa guauuacuuu ugacgacucu cuagccaaua aagugcuagu uuacgcaaca     1860

gcaaaccaag aaaaaggaca aagaacacca uaccaagcac uagauucaau ggaugaugcu     1920

uggucauuuc gagaacuaaa agcauuuguc agagaaagca aaacacuguc aaauaaaaag     1980

aaagaauacu uacugacaga agaagauaua agcaaauuug acguccgaaa gaaauucaua     2040

gaaaggaauu uaguagacac aagauacgca agccgggugg uucuaaacgc acuacaagaa     2100

cacuuuagag cccacaaaau agacacgaaa gucuccgugg uaagggggca auucacuuca     2160

caacuaagaa gacacugggg aauagagaaa acacgagaua cauaucacca ucacgcagua     2220

gaugcccuga uuauugcggc cagcucccaa cucaacuuau ggaagaaaca gaaaaacaca     2280

uuaguaucuu auuccgaaga ccaauugcuc gacauugaaa ccggagaauu gauaucagac     2340

gacgaauaca aagaauccgu uuucaaagcu cccuaccaac acuuugucga caccuuaaaa     2400

uccaaagagu ucgaagauag uauacuauuc agcuaucaag uagauagcaa auucaacaga     2460

aaaauaagcg acgcgacuau auacgcgaca cgccaagcaa aagugggaaa agauaaagca     2520

gaugaaacau auguacucgg caaaauaaaa gauauuuaua cccaagaugg uuaugacgcc     2580

uucaugaaaa uuuacaagaa agauaaauca aaauuccuua uguauaggca cgacccacaa     2640

acauuugaaa aaguuauaga accaauauug gaaaacuauc caaauaaaca aaugaacgaa     2700

aaaggaaaag aaguuccaug caacccguuc cuaaaauaca aagaagaaca uggauacaua     2760

agaaaauacu cuaagaaagg aaacggccca gaaauaaaau cacuaaaaua uuacgauagc     2820

aaacucggaa accauauaga uaucacgcca aaagauagca auaauaaagu gguacuucaa     2880

agccuaaaac cuuggaggac agacguauac uuuaacaaaa acacagguaa auaugaaauc     2940

uuaggcuuaa aauacagcga caugcaauuc gaaaaaggca caggaaaaua uucuauaucc     3000

aaagaacaau acgaaaauau aaaaguacga gaaggcguag acgaaaacuc agaguucaaa     3060

uuuacccuau auaaaaacga cuuacuguua cuuaaagacu cagaaaacgg agaacaaauu     3120

uuauuacguu ucacaucaag aaacgacacu uccaaacauu auguagaacu aaaaccauac     3180

aacagacaaa aauucgaagg uuccgaauau uuaauaaaaa gccucgguac agucguaaaa     3240

gguggccgau gcauaaaagg uuuaggaaaa uccaacauua gcauauacaa aguccgcaca     3300

gacgugcuag gaaaccaaca uauaauuaaa aacgaaggcg auaaaccaaa acuagauuuc     3360

uga                                                                   3363


<210>  5098
<211>  3363
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5098
augggacucg auauaggggu cgccagugua ggcuucucag uacuagauau agacaaaggc       60

aaaguaauug aacuaggggc aaggcuguuc ucagcaaccg uagcagcugg aaaccaagau      120

agaagggaca ugagaggagc acgcagacuc cugaauagaa acaaacaaag acgccaagac      180

acugggaaac uuuuuaaaaa auuuggacua auugaugacu ucgauaaggg aucauucuac      240

gauaacuuca accaaaaccu uaacccauau gaacuaaggg uaaaaggccu aaccgaacaa      300

cuaacaaaag aagaguuagc agaaaguuua uaccaaauag ucaaacaucg cggaauaagc      360

uaugcacuca aagacgcaga cguagaugaa ggaggaacag acuacagcgu aagucucaag      420

auaaauucuc aagaacucgc cgaaaaaacc cccgcacaaa uacaauuaca aagauuaaau      480

gacuacggaa aaguuagggg acaaguagua auaggagaug acccagacaa ccaaaaaguc      540

cugcuuaacg uguuccccac cagugcauac gaaaaggaag ccaaacaaau aauagcaacc      600

caacaacaau ucuacccaga aaguuuaacg gauaaguuua cugaagaaua uugucaaaua      660

uuaacacgca aaagagacua uuucgucggg cccgggaacg aaaaaagcag aaccgauuau      720

ggaauauaca aaacugacgg acguacacuc gauaacuuau ucgaagaauu gauaggacau      780

gauaaaauuu auccagaaga guuaagagca ucugcugcua guuauacagc acaauuauuu      840

aauguuuuaa augaucuaaa caaccuaaga auuuugaauu augaagacgg uaaacuaaca      900

aaagaagaca aagagaaaau aaucgcagaa auuaaaaaua auacgacaac aauaaauaug      960

cuaaauguaa ucaaaaaagu ugcaggcugu ucaaaagaug auauaaaagg auuuagagua     1020

aacgaaaaag acaaaccuga aauaucaucc augccugugu acagaaaaau ccauaaagau     1080

uuguuaaaag cgggaguaga cauaucagau uggccaguag aauucauuga cgagcuaagc     1140

uuuauucuaa cacuuaauac agaaaaugga gaaauaagaa aacaacucaa caauagacuc     1200

gcaccaaaau uugauuuccu caacgcugau uuaauacaac ucauaauaga uaacaaggac     1260

ucauucgaaa uaaaaaccaa caauaagugg caucgauucu ccgugaaaac aaugaauaaa     1320

uuaauuccag aaaugaugga aaggccagua gaacaaauga cauuauuaaa cgaaauggga     1380

uuaguaaaaa aagauaagaa aagguuugaa aauaauaaau aucuaccgua caaagaaaua     1440

gcaaaagaua uauuuaaccc uguagcuucc aaaucaguaa gggaagcuuu aaaaauagua     1500

aacgcaguuc ucaaaaaaua cggacacaua gauuaccuag uuguagaaau gccgcgcgau     1560

aaaaacuuaa aagaagaaca agauaauaua aaagaauucc aaaacaaaaa caaaaaagca     1620

aaagaugcug ccuucgaggc uuuuguaaaa agcguuggau cggaacaaag aguuaaagaa     1680

gcgcuaagca aaaaccgaaa acuccaaaug aaaaugagac uuugguacca acaacaggaa     1740

aucgacccuu auaauggaaa aacaaucgac gcgaccgauc uaaucaacaa ucccgacaaa     1800

uuugaaauag accacauaau uccgcagagc aucuccuuuu acgauuccau aaacaauaaa     1860

acccuguguu ucgcaagcau gaaucaaguc aaagggcaaa aaacaccaua cgaauucaug     1920

cuagaaggac auggacaauc auacgacaaa uuuaaagcaa cagugauggc aaauaagaac     1980

uucggaaaag ccaaacgagc aaacuacuua uuugaagaaa auguaucuga cauugaaacc     2040

aggaaaagau uccugucucg aaacuugguc gacacaaggu acagcucuag aguaguacua     2100

aauucacucc aagacuuuuu cagagaaaag ucugcagaca caaaaguaac cgucauaaga     2160

gggaaauuca cuagcaacau gcgcaaacau uggcacauag acaaaaccag ggaaaccuuc     2220

caccaccacg caauagaugc aucaauuaua gcagcuacac ccuuccuacg cauguggaaa     2280

aaagguggca caaucuuccc aguaaaaguu ggcgaggaau caauagacau agaaacagga     2340

gaaauauugg acgacaaaaa cuuugauaaa gcaauguacg aagagccuua cucaggauuc     2400

guuucagaaa uaaugaacgc ugacgacaga aucaaauuuu cacaccaggu agacaaaaaa     2460

augaaccgua aagucucgga ugccacaauu uacaguacaa gaacagguaa acuugcgaaa     2520

gacaaaaaag acgcagaaua cauaguggcg aaaguuaaag auauauauag uguagacggu     2580

uuuaagaaau uuaaaaaagu auacgauaaa gacaagacca aauuccugcu auacaaauac     2640

gacccaagaa cauuuaguaa acuagagcga auaauaucug acuguccgga uaaaguagaa     2700

aaaguacaaa caaacggaaa agucaaagca guagacauau caccauuuga gauguacagg     2760

agagaccacg guaugauuaa aaaauauagu aaaaagggca acgguccugc aaucaaacaa     2820

cuaaaauacu uagacaaaaa acugggcucc cauauagaua ucacgccggc uaacgcaaac     2880

ggaaaacacg uaauccuaca aaguuuaaaa ccauggagga ccgacguaua ccuaaaccau     2940

gagacaggag aauacgaaau cauggguauc aaauacucag acuuaaaguu caauaaaaau     3000

gaaggauaug ggauaaaaaa agacaaauau uuagaaauaa aaaaagucga agaaguauca     3060

gaaaaauccg aauucauguu cagccuuuau agaaaagaca gaguaaaagu ccaagauaug     3120

aaaacagggg aaagcguuga acuucuauuc ugguccagaa acuucagcaa caaaaaauac     3180

gccgaacuaa aaccaauuag ucaagcagaa aacgauaaga aacuuccagu auacggaaaa     3240

ggacgccuaa uaaaaagauu aauaccaaaa aacugcaaaa uauggaaagu uaacacaacu     3300

auauuaggcg acccauacua ucuggaaaaa gaaucagauu cgccuaaaga uauacuggac     3360

uga                                                                   3363


<210>  5099
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5099
augagcgauc uaguuuuagg acuugacaua ggaauaggca gcgucggagu aggcaucuua       60

aauaaaguaa caggugaaau aauacauaaa aauucacgua ucuuuccagc agcccaagca      120

gaaaauaacu uaguucgccg gacaaacaga caaggccgaa gacucacaag aaggaagaaa      180

cacagaagag ucagacuaaa ccgacucuuc gaagaaucag gacugaucac agauuucaca      240

aaaaucucaa uuaaccuaaa cccauaucaa uuacgaguga aaggacuaac agaugaauua      300

agcaacgaag aacuauuuau ugcccuuaaa aacaugguaa aacaucgagg uauaucauac      360

uuagaugacg cgagcgacga uggcaauuca agcauaggag acuacgcaca aauagucaaa      420

gaaaacagua aacaauuaga aacaaaaaca ccaggucaaa uccaacucga aagauaucaa      480

acuuaugggc aauuacgugg cgauuuuacc guagagaaag auggaaagaa acauagauua      540

auaaauguuu ucccaacauc agcauaucgu ucagaagcac uuagaauauu acaaacacag      600

caagaguuca auccccaaau aacagaugaa uuuguaaaca gauaucuaga aauauuaacc      660

ggaaaacgaa aauacuacca ugguccagga aacgaaaaau caagaaccga uuaugguaga      720

uaucgaacua acggagaaac auuagauaac auuuucggua uacugaucgg aaaauguacc      780

uuuuauccag augaguuccg agcggcuaaa gcaagcuaca cagcccaaga guucaacuua      840

cuaaaugauu uaaauaaccu aacggugcca acagaaacca agaaacugag caaagaacag      900

aaaaaccaaa ucauaaacua uguuaaaaac gaaaaagcaa ugggaccagc uaaacuguuu      960

aaauacauag caaaguuauu aagcugugac guagcugaca uaaaaggaua ccgaaucgac     1020

aaaagcggca aagccgaaau acacacauuc gaagcauaua ggaaaaugaa aacacuggaa     1080

acgcuggaca uagaacaaau ggaucgagaa acauuagaca aacucgcuua cguacuuaca     1140

cuuaacaccg aaagggaagg aauccaagaa gcguuagaac augaguucgc ugacggauca     1200

uucucacaaa aacaaguaga ugagcuaguc caauuccgaa aagcuaacag uaguauauuc     1260

gguaaaggcu ggcacaacuu uagcguaaag cuaaugaugg aauugauccc agaacuuuau     1320

gaaacuagcg aggaacaaau gacaauauua acaagacucg gaaaacagaa aacuacauca     1380

ucaagcaaua aaaccaaaua uaucgaugaa aaauuguuaa cagaagaaau cuauaaccca     1440

guaguggcca aguccguaag acaagcaaua aaaauaguua acgcagcaau uaaagaauac     1500

ggagacuucg acaauauagu uaucgaaaug gcaagagaaa caaacgaaga cgacgaaaag     1560

aaagcuauac agaaaauaca aaaggcuaac aaggaugaaa aagaugcggc aaugcuaaag     1620

gccgccaacc aauacaacgg uaaagcagaa uuaccccaua gcguuuuuca cggccauaaa     1680

caacuagcaa caaaaauccg ucucuggcau caacaaggag aacgauguuu auacacggga     1740

aaaacaauau caauacauga ccucaucaau aauucaaacc aauucgaagu ggaucauaua     1800

cuaccacuau caauaacauu cgacgacucu uuagcaaaca aaguccuagu auacgcgaca     1860

gcaaaucaag aaaaaggaca aagaacacca uaccaagcau uagacuccau ggacgacgca     1920

uggagcuuca gagagcugaa agcauuugua agggaaagua aaacacuaag caacaagaag     1980

aaagaauacu uauuaacaga agaagauauu ucgaaauucg acguccgcaa gaaauucauc     2040

gaaagaaauc uaguagauac aagauacgca ucaagaguag uauuaaacgc ccuccaagaa     2100

cacuuuagag cacauaaaau agauacaaaa guuucaguag uaagaggaca auucacaagc     2160

cagcuacgac gacacugggg aauagagaaa acaagggaca cauaucauca ucacgcugug     2220

gaugcacuaa ucauagccgc aucaucacaa cuaaauuugu ggaagaaaca gaaaaauaca     2280

uuagugagcu auucagaaga ccaauuauua gacauagaaa cuggagagcu aauaagcgau     2340

gaugaauaca aagaauuugu auuuaaagca ccauaccaac acuuuguuga uacacuaaaa     2400

agcaaagaau uugaagauuc uauauuauuu uccuaccaag uagauucaaa auucaacaga     2460

aaaauaucug acgcuaccau auacgcuacc agacaagcca agguaggcaa agauaaagca     2520

gacgaaacgu acguacucgg aaaaauaaag gacauauaua cacaagacgg auaugacgca     2580

uuuaugaaaa uauauaagaa agacaaaagc aaauuccuaa uguacagaca cgacccgcaa     2640

acauucgaaa agguaauaga accaauucua gaaaacuacc caaacaagca aaugaacgaa     2700

aaaggaaaag aaguuccaug caaccccuuc cucaaauaua aagaagaaca cggguacaua     2760

cgaaaauaua guaagaaagg caacggaccg gaaauaaaaa guuuaaaaua cuacgacuca     2820

aaacucggga accauaucga uauaacucca aaagacagca auaauaaagu aguccuacaa     2880

aguguuagcc cauggcgagc agauguauau uuuaacaaca caacagguaa auacgaaaua     2940

cuaggacuca aguacgccga ccugcaauuu gaaaaaggca cgggaacaua uaaaauauca     3000

caagaaaaau acaaugauau aaagaagaaa gaggguguag auagugauag cgaauuuaaa     3060

uucacauuau acaaaaauga uuuacucuua gucaaagaaa cagaaacaaa agaacaacaa     3120

uuauuccgau uuuuaucaag aacuaugccu aaacaaaaac auuauguuga guuaaaaccc     3180

uaugacaaac aaaaauuuga agguggagag gcacuaauaa aaguacuagg aaauguggcu     3240

aacggagggc aauguauaaa aggucucgga aaaucaaaua uaucgauaua caaaguuaga     3300

acagacguau uaggcaacca acacauaauc aaaaacgaag gagauaaacc uaaacucgau     3360

uuuuga                                                                3366


<210>  5100
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5100
augaacggcc uaguacuugg auuagacauc ggaauagcuu caguaggugu aggcauacua       60

gagaaaaaca gugggaaaau uauccacgca aacaguagau uauucccagc ggccacagcg      120

gacaacaacg uagaaagacg uaaaaacaga caagccagaa gacuaaacag acgaaagaaa      180

caccgaggug ugagauucca ggaccuguuc gaagacuacg gauuauuaac ugauuuuucu      240

aaaguaucga uaaaucugaa uccauacagg uuaagaguag acggccugaa caaacaacua      300

acgaaugaag aacuauucau cgcucugaaa aacaucguua aaagacgcgg cauaaguuau      360

uuagacgacg cuucagaaga cggaggaaca gucaguagcg auuauggaaa agccgucgaa      420

gaaaaccgaa aauuauuagc agaacaaacc ccaggacaaa uacaacuuga aagauucgaa      480

aaauauggac aacugagagg agacuucaca gucguagaaa acggcgaaaa augcagauua      540

auaaauguau ucagcacaag cgcauacaag aaagaagccg aaagaauauu gagaaaacaa      600

caagaguuua auaguaaaau aacggacgag uucaucgaag auuaucuaaa aauacugaca      660

ggcaaaagga aauacuauca cggacccgga aacgaaaaau caaggacaga uuauggccga      720

uucagaaaag acggaaccac guuagauaac auauucggca uacucauugg aaaaugcaca      780

uuuuacccaa acgaauacag agccucaaaa gcauccuaua cagcccaaga guucaaccua      840

cugaaugacu uaaauaaccu aagcgucccu acggaaacca agaaacuaag ugaagaacag      900

aagaaaacaa uaauagaaua ugccaaaucg gcgaaaacac uaggcgcuuc aacgcuucuu      960

aaauacauag caaaaaugau agacacaagc guagaccaaa uaagaggaua uagaguagac     1020

guaaauaaua aacccgaaau gcacacauuu gaaguauaua gaaaaaugca aagucuagaa     1080

acaauaucug uaggcgaacu aagcagaaau guucuagaug aacuugcaca uauacuaaca     1140

uuaaauaccg agagagaagg aauagaagaa gcuaucaaua cuaaacuaaa agauaguuuu     1200

ucucaagacc aaguauuaga auuaguacaa uuuaggaaaa acaacucauc uuuauuuuca     1260

aaaggcuggc acaacuuuag ucuaaaacuc augauggaau uaauaccaga gcuauaugag     1320

acaucagaag aacaaaugac aauuuuaaca agauuaggaa aacagaaauc aaaagaaacu     1380

ucaaaaagaa guaaauacau agacgaaaaa gaauuaacgg aagaaaucua caauccagua     1440

guagccaaaa guguucgaca agcaauuaaa auaaucaaug aagcaacaaa gaaauacgga     1500

auauucgaca auauagucau agaaauggcc cgagaaaaug acgaagagga cgcaaagaaa     1560

gacuacauaa aacgccaaaa agcuaaucaa gaugagaaaa acgccgcuau ggaaaaagcc     1620

gcauuucaau acaacggaaa gaaagaauug ccagacaaca uauuccaugg ccauaaagaa     1680

cuagcaacca aaauacgacu guggcaccaa caaggggaaa aaugccucua uaccggaaaa     1740

acgauaccca uaucggaccu aauacacaau caauacaaau augaaauaga ccauguccuu     1800

ccauuaagcc uaucguuuga ugacucacug agcaauaaag ucuuaguacu agcuaccgca     1860

aaccaggaaa aaggccaacg cacgccauuu caagcacuag auucaaugga ugacggaugg     1920

agcuacagag aguucaaaag cuauguaaaa gaaucaaaau uacuaggaaa caaaaagaaa     1980

gaauaucuac uaacagaaga agauauaucu aaaaucgaag uaaaacagaa auucauagaa     2040

cgaaacuuag ucgauaaucg guacagcuca agagucgucc ucaaugcccu acaagacuuc     2100

uacaaagcuc acaaauuuga caccacaaua ucagucguca gaggacaauu cacgucgcaa     2160

uugaggcgaa aauggggauu agaaaaauca cgagaaacgu accaucacca cgccguagac     2220

gcguuaauaa ucgcagcauc aucucaauug agauugugga agaaacacaa caaucccuua     2280

aucucauaua aagaaggaag auucauagac ucagaaacag gugaaauuau aagccuaagc     2340

gaugacgaau acaaagagcu agucuucaaa gcacccuaug aucacuucgu ugauacuuua     2400

agaaguaaaa cauuugaaga cuccauauua uuuaguuacc aaguagauuc aaaauauaau     2460

agaaaaauuu cagacgcaac aauauaugca acaagaaaag caaaacuuga caaagacaaa     2520

ucggaagaaa cuuacgucuu aggaaaaaua aaagauauau acucacaagc aggauaugau     2580

gccuuuauaa aaaucuacaa uaaagacaaa ucuaaauucc ucauguauca caaagauccu     2640

caaacauucg aaaaagucau agaagaaaua uuaagaacau acccaucuaa agaacuuaac     2700

gauaaaaaca aagaaauacc gugcaacccg uucgagaaau aucgacaaga aaauggucca     2760

auuagaaaau auaguaagaa agguaacggc ccagaaauaa aauccaugaa auacuacgac     2820

aaaaacaucc cugaguccuu caucaauaua accccgucua auucaaauaa caaagucuua     2880

cucagacaau uauauccaug gcgaacagau guuuacuuua auccacaaac agacaaauau     2940

gaacuacuug ggcuaaaaua cgccgacuua caauucgaaa aagcaacagg aaaauauagc     3000

auaacuauag acaaguauaa ugaaaucaaa gaaaaagagg gaguaucaaa agaaucagag     3060

uucaaauuua cacuguacaa aaacgaccua cuauuaauua aagacaccaa aaauaacgaa     3120

gaacaaauau uccgcuucuu aucacguaca augccaaauc aaaaccacua uguagaauua     3180

aaaccuuacg auaaagggaa cuucgaaggc aaagaaaaga uuaaagucuu caaaaugaca     3240

ucuaauggac gauguauaaa aggcuuagga aagaaauacc uuucaauaua uaaaguaaaa     3300

acggacauac uaggaaacaa acauuucaua aagaaagaag gagacgaacc uaaauugaaa     3360

uucuga                                                                3366


<210>  5101
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5101
augaauggac uuguucuggg ucuagacaua ggcauagcau caguuggcgu agggaucuua       60

gaaaagaaca gcgggaaaau aauacacgcu aacucucguc uuuucccagc agcaacagca      120

gauaacaacg uugaaagacg aaagaaucga caagcccgaa gauuaaauag aaggaagaaa      180

cacagaggag uaagauucca agaccuauuu gaagacuacg gauuauuaac agauuuuagu      240

aaagucagca ucaacuuaaa cccauauaga uuaagaguag acggacuuaa ccagcaauua      300

acuaaugaag aauuauuuau cgcauugaaa aacauaguaa aacggcgcgg uauaagcuau      360

cuagacgaug ccagugaaga ugguggggca guaaguucug auuauggaaa agcaguagaa      420

gaaaauagaa aacuacuugc agaacaaacu ccuggacaaa uacaacuaga aagauuugaa      480

aaauauggac aaguaagagg cgacuucacu guaguagaaa auggcgaaaa acguagauua      540

auaaaugucu uuaguacgag cgcauauaga aaagaagcug agaaaauacu aagaaaacaa      600

caagaguuca auaaucaaau aacagacgag uucaucgaag auuacuuaaa aauucucacu      660

ggaaaacgaa aauacuacca uggacccgga aacgaaaaau caagaacaga cuauggacga      720

uucagaacag acggaacaac acuagauaac aucuucggag uauuaauagg aaaaugcaca      780

uuuuacccca augaauacag agcaucaaaa ucaucauaca cugcacaaga guucaaucua      840

cuaaacgacu ugaacaauuu aacaguacca acugaaacca agaaauuauc agaagaacag      900

aagaaaguua uagucgaaua cgcuaaagaa gcaaaaaccu uaggagcaag cacauuauug      960

aaauacauag uaaaaaugau agacgcaucc guagaccaaa uaaguggcua ucguguagau     1020

guaaauaaca aaccagaaau gcacaccuuu gaaguauaua gaaaaaugca aucacuagaa     1080

acgauaagug ucggcgaacu uucuaggaac guuuuagacg aauuagcgca uauauuaacu     1140

uuaaauacag aacgcgaagg uauugaagaa gcaauaaaua guaaacuuau ggguguauuu     1200

ucacaagauc aaguccucga acuuguucaa uuccguaaaa acaauucauc auuauucucu     1260

aaaggauggc auaacuuuag cuuaaaauua augauggaau ugauaccuga auuauacgaa     1320

acaucugaag aacaaaugac gauacucacu cgacugggaa aacagaaaag caaagaaaca     1380

agcaaacgaa caaaauacau agaugaaaaa gaacucaccg aagaaauaua caacccagua     1440

guagcaaaaa guguacguca agcuauaaaa auaauaaacg aagcaacaaa gaaacaugga     1500

auauuugaua auaucguaau agaaauggcg cgcgaaaaca augaagaaga ugcaaagaaa     1560

gauuacauaa aaagacaaaa agccaaucaa gacgagaaaa acgcugcgau ggaaaaagcu     1620

gcuuuucaau acaauggcaa gaaagaacua ccuggcaaua uauucaaugg ccacaaagaa     1680

uuagcaacaa aaauucgauu auggcaucaa caaggggaaa aaugccuaua uacaggcaaa     1740

acaauaccaa ucucagauuu gauucauaau caauacaaau augaaauaga ccacauacua     1800

ccacuuagcc ucagcuucga cgacagccuc ucuaacaaag uucuaguuca cgcgacggcu     1860

aaccaagaaa aagggcaacg aacaccauuc caggcuuuag auucaaugga ugacgcaugg     1920

ucauaccgag aguucaaauc cuauguaaaa gaccucaaau cgcucucaaa uaaaaagaaa     1980

gacuaucuau uaacagaaga agauauauca aaaauagaag uuaaacaaaa auucauagaa     2040

cgaaaccucg uagauacccg auauagcuca agaguagucc uaaacgcacu gcaagacuuc     2100

uauaaaucac aucaauucga cacuacaauc agcguuguaa ggggccaauu uacaucccaa     2160

cuccgacgaa aauggggccu agaaaaauca agagaaaccu accaccacca cgcaguugau     2220

gcacuaauca uugcagcauc aucacaacug agacuaugga agaaacaaaa caacccucua     2280

auaacauaua aagaaggaca auuuauagac agucaaacag gagaaauaau auccuuaacg     2340

gacgacgaau acaaagaauu aguguucaaa gcaccuuaug aucauuucgu cgacacauua     2400

cacucaaaaa cuuucgaaga cggaauacua uucuccuauc aaguagacuc uaaauacaac     2460

cggaaaauuu cagacgcaac aauauaugca acuagaaaag ccaaacuaga uaaagacaaa     2520

agugaagaaa cauacguauu ggguaaaauu aaagauauau acucacaagc agguuacgau     2580

gccuucauua agauauauaa uaaagacaaa agaaaauucu uaauguauca uaaagacccc     2640

caaaccuuug aaaaaguaau agaagaaaua uuaagaacau aucccaguaa agaaagaaac     2700

gacaaaaaca aagaaguacc uuguaacccg uuugaaaagu aucgucagga aaacggucca     2760

auaagaaagu auucaaagaa aggaaacgga ccagaaauaa aauguuuaaa auacuaugac     2820

aauaaacugg gcaauuauau agacauaucc ccggauggau ccgacaauca aguaguacua     2880

caaucuuuaa aaccauggag aacagaugua uacuucaacc accagacuaa gaaauacgaa     2940

cuaaugggcu ugaaauacag ugacuuauca uuugaaaaag gaagcggaaa auacucuaua     3000

ucaaaugaaa aauauaauag cauaaaacga aucgaaggug uaaaugaaca aagcgaguuc     3060

aaauucacuu uauacaaaaa cgaccuaauu cuaauuaaag auucagaaaa caaugaacaa     3120

aaacucuuca gguuuaauuc aagaaacgac acagccaaac auuacguuga acucaaacca     3180

uacgauaaag caagauuuga cggccaacag gaacugauaa caauauuagg aaauguagca     3240

aaaggaggac aauguuuaaa aggauuaaau aaaucaaaua ucaguauauu caagguaaaa     3300

acugacguac uugguaagaa acacauaauc aaaaaggaag gggacgaacc aaaacucaag     3360

uucuga                                                                3366


<210>  5102
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5102
augagcgacc uaguacuagg guuagacaua ggaauaggaa gcgugggagu aggaauauua       60

aacaaaguaa caggagaaau aauacauaaa aauucaagaa uauuucccgc agcccaagca      120

gaaaacaauc uuguaaggag aacaaacaga caaggaagaa ggcuaaccag acgcaagaaa      180

cauagaauag uacgucuaaa uagacuauuu gaagaaucgg gauuaaucac agauuuuaca      240

aaaauuagca uaaaccuuaa cccauaucaa uugagaguaa aaggucuaac agacgaacua      300

ucaaacgaag aauuauuuau agcacuaaag aauaugguaa aacaucgagg aauaucuuac      360

uuagacgacg cguccgacga cggaaacucc ucuguaggag acuaugcaca aauaguaaaa      420

gaaaacucaa aacaacuaga aacuaaaacg ccaggacaaa uucaacuaga aagguaccaa      480

acauacggac aacuacgagg agauuucaca gucgaaaaag auggcaagaa acaucgacua      540

aucaauguau ucccaacauc ggcauacagg ucagaggcuu uaaggauacu gaaaacccaa      600

caagaguuca auccacaaau aaccgaugaa uuuauaaacc gcuacuugga aauacuaaca      660

ggaaaaagaa aauauuacca uggcccuggc aaugaaaaau cacgaacuga uuauggucga      720

uacagaacua auggagaaac auuagauaac auauuuggca uauugauugg aaaaugcaca      780

uucuacccag aagaauuuag agcggcaaaa gccucauaca ccgcacaaga guucaaucuc      840

cugaaugauu uaaauaaccu gacaguacca acagaaacaa agaaacucuc uaaagaacag      900

aaaaaccaaa uaauaaacua cgugaaaaau gaaaaagcaa ugggaccagc caaauuguuc      960

aaauauauag caaaauuacu aucaugugac guagcagaca uaaaaggcua cagaauagac     1020

aaaucaggga aagcagaaau ccacacguuc gaagccuauc gaaaaaugaa aacacucgaa     1080

acucuagaua uugaacaaau ggacagagaa acgcuugaua aauuagccua uguucuaacc     1140

cuaaacaccg aacgagaagg aauacaagaa gcacuugaac acgaauuugc agauggguca     1200

uucucacaaa aacaaguaga cgagcuaguc caauuuagaa aagcuaacag uucaauauuu     1260

ggaaaaggau ggcauaacuu cuccguaaaa cuaaugaugg aauuaauucc cgaacucuau     1320

gaaacuucgg aagaacaaau gaccauacuu acuagauugg gaaaacagaa aaccacaucc     1380

ucuagcaaca aaacaaaaua cauugaugaa aaauuauuaa cugaagaaau auauaaucca     1440

gugguagcaa aaucaguuag gcaagcuauu aaaauaguaa augcagcuau aaaagaauac     1500

ggagacuuug acaacauugu caucgaaaug gcuagagaaa caaaugaaga ugacgaaaag     1560

aaagcaauuc agaaaauaca aaaagcaaac aaagaugaaa aagacgcagc aaugcuaaaa     1620

gcagcaaacc aauauaacgg aaaagcugaa uuaccacauu cagucuucca cggacauaaa     1680

caacuagcga cuaagauccg ccucuggcac caacaaggag aaagaugccu auacacaggc     1740

aaaaccauau caauacacga ccuaauaaau aauccaaacc aauucgaagu agaccacauc     1800

cuaccauuau ccauaacauu cgacgauucc cuagcaaaca aaguucuagu auacgcaaca     1860

gcaaaccaag aaaaaggaca acgcacccca uaucaagccu uagacagcau ggaugacgca     1920

ugguccuucc gagaacuaaa agcauucgua agagaaucca agacauuaag caacaaaaag     1980

aaagaauauc uacuaacaga agaagauaua aguaaauucg auguccaaaa gaaauucaua     2040

gaaagaaacc ucguagauac aagauaugca ucacgaguag uauuaaacgc ucuccaagaa     2100

cacuuuagag cacacaaaau agacaccaaa guaagcguag uucgggggca auucaccucu     2160

caauuaagaa ggcauugggg aaucgagaaa acacgagaua cauaccacca ucaugcggua     2220

gacgcccuaa uaauagcugc uaguucgcaa cuaaaccuuu ggaagaaaca gaaaaacacc     2280

cuaguaucau acucagaaga ccaauuacua gauauugaaa caggugaacu cauaagcgau     2340

gaugaauaca aagaauccgu uuucaaagca cccuaccaac acuucguuga uacacuaaaa     2400

agcaaagagu ucgaagauuc uauccucuuc ucauaccaag ucgacucuaa auucaaucgc     2460

aaaauaucag acgcaacaau uuacgcuaca agacaagcca aaguaggaaa agacaagaaa     2520

gacgaaacau auguuuuagg uaaaauuaaa gacauuuaua cucaagaugg cuacgaugcc     2580

uucaugaaaa uauauaagaa agacaaaucg aaauuuuuaa uguacagaca cgacccccaa     2640

acauuugaaa aaguaauaga accgauacua gaaaacuacc cuaacaaaga aaugaacgaa     2700

aaaggaaaag aaguccccug caacccauuu uuaaaauaua aagaagaaca cggcuauaua     2760

aggaaauauu caaagaaagg uaauggccca gaaauaaaau cauuaaaaua uuacgacuca     2820

aaacugggaa accauauaga cauaacaccc aaaaacucua acaacaaagu cguacuacaa     2880

ucaguaucac ccuggagagc ugauguauau uuuaacaaaa caacuggaaa auaugaaauc     2940

uuaggcuuaa aauaugcaga uuuacaauuu gaaaaaggua ccgggaccua uaaaaucuca     3000

caagaaaaau acaacgauau uaaaaagaaa gaaggagucg auagugacuc agaauuuaaa     3060

uuuacuuuau acaaaaacga ucuacuauua guaaaagaca cagaaaccaa agaacaacaa     3120

cuauuuagau ucuuaucaag aacuaugccc aaaaagaaac acuaugucga auuaaaacca     3180

uaugacaaac agaaauuuga aggaggcgaa gcgcuaauaa aaguucuagg gaacguagca     3240

aacucaggcc aauguauuaa gggcuuagca aaaucaaaca ucucuauuua uaaaguaaga     3300

acugacgugc ucggaaauca acacauaauc aaaaacgaag gagacaaacc aaaacuagac     3360

uucuga                                                                3366


<210>  5103
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5103
augucagacc uaguucuagg acuagauaua ggaauaggaa gcgucggcgu agggauauua       60

aacaaaguaa caggugaaau aauucauaaa aacagucgga uauucccggc agcccaagcu      120

gaaaacaacc uuguuagaag aacaaauagg cagggaagaa gauuaacacg aagaaagaaa      180

cacagaagag ugagacuaaa uagacuauuc gaagaaucag gauuaauaac agauuuuaca      240

aaaauaagua uaaaucuuaa uccauaccaa cuacgaguaa aaggccuaac agaugaacuc      300

agcaaugaag aauuguucau agcauuaaag aacaugguaa aacaucgcgg uauaucuuau      360

uuagacgacg caucggacga ugguaacagu ucgauagggg auuacgccca aauaguuaaa      420

gaaaacucaa agcaacuaga gacaaaaaca ccuggacaaa uacaacucga aagauaucag      480

acauauggac aacuaagagg agauuucaca guagaaaaag acgguaagaa acacagauua      540

auaaacguau ucccgacauc agcauauaga agcgaggcac uaagaaucuu gcaaacucaa      600

caagaauuua auccgcaaau aaccgacgaa uuuaucaacc gauauuuaga gaucuuaacc      660

ggaaaacgaa aauacuacca cggaccaggc aaugaaaaau cgcgaacaga uuacggucgu      720

uacagaacaa guggggaaac ucuggacaac auauucggaa uauugauagg uaaauguaca      780

uuuuaccccg acgaguucag agcagcuaaa gcaagcuaua cugcacaaga guuuaaccuc      840

cuaaacgacc uaaauaaucu aacgguuccu acugaaacaa agaaacuuuc caaagaacag      900

aaaaaccaaa ucauaaacua ugugaaaaac gaaaaagcca ugggaccagc caaacuauuc      960

aaauauauug caaaacuauu aagcugugau guggcagaca uuaaaggcua cagaaucgac     1020

aaaucuggaa aggcagaaau acauacguuc gaagcauauc gaaaaaugaa aacccuugaa     1080

acacuagaua uagagcaaau ggauagagaa accuuagaca aacuugcuua uguacuaacc     1140

uuaaacaccg aaagagaagg aauacaagag gccuuagaac augaguucgc agauggauca     1200

uucagccaaa aacaaguuga cgagcuagug caauuuagga aagcaaauuc uucaauuuuc     1260

ggaaaaggau ggcacaacuu uaguguaaaa cuaaugaugg aacugauacc agaauuauau     1320

gaaacaucag aagaacaaau gacaauacua acuaggcuug ggaaacagaa aacaaccagu     1380

aguucaaaca aaacuaagua cauugacgaa aaauuauuaa cggaagaaau auacaacccc     1440

guaguggcga aaucaguaag acaagcaauc aaaaucguaa augcagcuau uaaagaauac     1500

ggagauuuug auaauauugu gauagaaaug gcacgagaaa caaaugaaga cgaugaaaag     1560

aaagcaauac agaaaauaca aaaagcaaac aaagacgaaa aagacgcugc aaugcuaaaa     1620

gcagcaaauc aauacaaugg aaaagcagaa uuaccccacu caguauucca ugggcauaaa     1680

caacucgcua caaaaauaag acuauggcac caacaaggug aaagaugcuu auacacuggc     1740

aaaaccaucu caauucacga ucuaauaaau aauuccaacc aauucgaggu agaucauaua     1800

cucccccuau cuauaacuuu ugaugacuca uuagcaaaua aaguauuagu auaugcaaca     1860

gcaaaucaag aaaaaggaca aagaacacca uaccaagcac uagauagcau ggaugaugca     1920

uggucguuca gagaguuaaa ggccuuugua agggaaagca aaacauuauc uaacaagaag     1980

aaagaauacu uguuaacaga agaggacauu ucaaaauuug acguccgcaa gaaauuuaua     2040

gaacgcaauu uagucgacac acgauaugcc ucccgaguag uacuaaaugc uuuacaagaa     2100

cacuuccgag cacacaaaau cgacaccaaa guaagcguag uaagagguca auucaccagc     2160

caacucagaa gacacugggg aauagagaaa acaagagaca cauaccauca ccaugcaguu     2220

gacgcauuaa uaauugcugc aucaucucaa cuuaaucuau ggaagaaaca gaaaaauacg     2280

uuaguauccu acagugaaga ucaacuccuc gacaucgaaa caggcgaauu aauuagcgau     2340

gacgaauaca aagaaagcgu auuuaaagcg ccuuaccaac acuucguaga cacauuaaaa     2400

ucuaaagagu ucgaagacag cauacuauuc aguuaucaag uagacaguaa auuuaauaga     2460

aaaauaucag acgcaacaau auaugccaca agacaagcaa aaguggguaa agauaaagcc     2520

gaugaaacau acguacuagg aaaaauaaag gacauauaua cccaagacgg auaugacgca     2580

uuuaugaaaa uauauaagaa agauaaaagc aaauuccuca uguauagaca cgauccccaa     2640

accuucgaaa aagucauaga acccauacuu gaaaacuacc cuaacaaaca aauuaacgaa     2700

aaagggaaag aaguaccaug uaacccauuc uuaaaauaua aagaagaaca cggcuacaua     2760

agaaaauauu caaagaaagg aaauggcccc gaaaucaaau cgcucaaaua cuaugacuca     2820

aaauuaggga accacauaga uauaacaccu aaagauagua auaacaaagu cguacuacaa     2880

ucagucagcc cuuggagggc agauguauau uucaacaaaa caacaggaaa auacgaaaua     2940

cuagggcuaa aauacgccga ucuccaauuc gaaaaaggaa ccgggacaua uaaaauaagu     3000

caagaaaaau acaaugauau caaaaagaaa gaagggguag acagcgauag cgaguucaaa     3060

uuuacauuau acaaaaacga ccucuuauua gucaaagaua cugaaacuaa agaacaacaa     3120

cuauucagau uccuuaguag aacaaugcca aaacaaaaac acuacgucga acuaaaacca     3180

uacgauaaac aaaaauucga aggaggcgaa gccuuaauaa aaguucuagg uaaugucgcu     3240

aacaguggac agugcaagaa aggacuagga aaaucuaaca uaaguaucua caaaguaaga     3300

accgauguau uagguaacca acauauaauu aaaaaugaag gagauaaacc aaaauuagac     3360

uuuuga                                                                3366


<210>  5104
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5104
augagcgacc uaguauuagg gcuugacaua ggaauaggau caguaggagu cggaauacuc       60

aacaaaguua caggagaaau aauacacaaa aacucgagga uauuuccagc cgcccaagca      120

gaaaacaacc ucguuagacg gacgaacaga caaggaagaa gacuaacgcg aagaaagaaa      180

caccgcagag uacgacuaaa ccgacuuuuc gaagaauccg gacuuauaac agauuucaca      240

aaaauaucaa uaaaucugaa uccauaucaa uuacgaguaa aaggguuaac agaugaacua      300

ucaaaugaag aacuauuuau agcccucaaa aacaugguaa aacaucgagg uauaucauau      360

uuagacgacg caucugauga ugguaacagc ucagucggag acuaugcaca aauagucaaa      420

gaaaacucaa aacaauuaga aacaaaaaca ccaggacaaa uacaacuaga aagauaucaa      480

acauaugguc aauuaagagg cgacuucacu gucgaaaaag augguaagaa acaccguuua      540

aucaauguau uuccaacaag cgcauaucga uccgaagcac uaagaauacu ccaaacucaa      600

caagaguuca acccccaaau uacugacgag uucguaaaua gauaccuaga aauucuaaca      660

ggaaaaagaa aauauuacca ugggccagga aaugaaaaau cacgaacgga cuauggaaga      720

uauaggacaa acggagaaac acucgauaac auuuuuggaa uauuaaucgg gaaaugcaca      780

uuuuauccag aagaauuuag agcagcuaaa gcaaguuaua ccgcacaaga guucaacuua      840

cuuaacgauc ugaauaacuu aacaguaccc acugaaacca agaaauuauc aaaagaacag      900

aaaaaucaaa uuaucaacua ugucaaaaau gaaaaagcga ugggaccagc caaacuauuu      960

aaauacauag cuaaacuccu uucaugcgau guagcagaua uaaaaggaua ccgaauagac     1020

aaauccggaa aagcagaaau ucacacauuc gaagcauaca ggaaaaugaa aacauuagaa     1080

acccuagaca uagaacaaau ggaucgugaa acauuagaca aacuagcaua cguacuaaca     1140

cuuaacacag aaagagaagg aauacaagaa gcauuggaac acgaguucgc ugacgguagc     1200

uuuucucaaa aacagguaga cgaauuagua caauucagaa aagcaaacuc aucaaucuuc     1260

ggaaaaggau ggcauaauuu cagugugaaa cugaugaugg aacuuauucc ugaacuauau     1320

gaaacgagcg aagaacaaau gacgauacua aaacgauuag gaaaacagaa aacaacuagc     1380

aguaguaaca aaacgaaaua uauagaugaa aaacuacuua cagaagaaau auauaaucca     1440

gucguugcga aaucaauuag acaagcaaua aaaauuguaa acgcagcaau aaaagaauac     1500

ggagauuuug acaauaucgu aaucgaaaug gcacgagaaa caaaugaaga ugaugaaaag     1560

aaagcgauuc agaaaauaca aaaagcgaac aaagacgaaa aagaugcugc uaugcugaaa     1620

gcagcgaacc aauauaacgg aaaagcugaa cuaccucacu ccguauucca uggucacaaa     1680

caacuagcaa caaaaauuag acuguggcac caacaaggag aacguugcuu guacaccgga     1740

aaaacaauaa gcauacacga ccuaauaaac aauccaaauc aauuugaagu agaucacaua     1800

uuaccccuau caauuacuuu ugacgauucc cuagcaaaua aagugcuagu auacgcuacu     1860

gcaaaucaag aaaaaggaca acgaacgcca uaucaagccc uagauucaau ggacgacgca     1920

uggucauuca gagagcuaaa agcauuugua agagaaagua aaacacuuuc aaacaaaaag     1980

aaagaauauu uauuaaccga agaggacauc ucuaaauucg auguacgcaa gaaauuuaua     2040

gaaagaaauc uaguagacac aagauaugca ucacgaguag uccuaaaugc ucuccaagaa     2100

cauuuuagag cacauaaaau agacacuaaa guaucagucg uaagaggaca auucacauca     2160

caauuaaggc gacauugggg aauugagaaa acaagagaca ccuaucacca ccaugcuguc     2220

gacgcccuua uaaucgcagc uaguucucaa uuaaacuuau ggaagaaaca gaaaaauacc     2280

cuaguaagcu auagcgaaga ccaacuuuua gacaucgaaa caggcgaauu aaucucugac     2340

gaugaauaua aagaaucagu uuucaaagcc cccuaccaac auuucguaga uacauuaaaa     2400

ucaaaagagu ucgaagacag cauucuauuu ucuuaucaag uagauuccaa auuuaauaga     2460

aaaaucucag acgcaacaau auacgcaaca agacaagcca aagucggcaa agacaagaaa     2520

gacgaaacuu auguacuagg aaaaauuaaa gauauauaca cacaagacgg guacgacgcc     2580

uuuaugaaaa uauacaagaa agauaaauca aaauuuuuaa uguaccgaca ugauccacaa     2640

acauuugaaa aagugauuga accaauauua gaaaacuacc cgaacaaaga aaugaaugaa     2700

aaaggaaaag aagucccaug caacccauuc cucaaauaca aagaggaaca uggauauaua     2760

cggaaauaua guaagaaagg aaacgauccu gaaauaaaaa gucuaaaaua uuacgacagu     2820

aaacuaggga aucacauuga uauaacuccc aaagauucaa auaauaaagu aguauugcaa     2880

uccgucaguc cuuggcgcgc agauguuuau uucaacaaaa cgacaggaaa auacgaaaua     2940

cuuggacuua aauaugcaga ucuacaauuc gaaaagggca caggaacuua uaaaauaagu     3000

caagaaaaau auaacgacau aaagaagaaa gagggaguag auagugauuc ggaguucaaa     3060

uucacucuau acaaaaauga ucuccuucuu guaaaagaca ccgaaacaaa agaacaacaa     3120

uuauucaggu uccuaagccg uacaaugccc aagcaaaaac acuacguaga acuaaaacca     3180

uacgacaagc aaaaauucga aggcggugaa gccuuaauca aaguauuagg uaacgucgca     3240

aacaguggcc aaugcaagaa aggacuagga aaaucaaaua uaagcauaua uaaaguacgu     3300

accgacgucc uuggaaacca gcauauaauu aaaaaugaag gcgacaaacc aaaacuagac     3360

uucuga                                                                3366


<210>  5105
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5105
augaauggac uuguacuagg auuagauaua ggaauagcau caguaggggu cggaaucuua       60

gaaaaaaaua gcgguaaaau cauacacgcc aauuccaggu uauucccugc ugcuacagcc      120

gacaacaaug uagagagaag aaaaaauaga caggcaagaa gacuucacag gagaaaaaaa      180

cacagaggag uacgauucca agaccuauuc gaagacuaug gauuguuaac ugacuuuuca      240

aaaguaagua uaaaucuaaa ccccuacaga cuacgugucg acggauuaaa ccagcaauua      300

acaaacgaag aacuguuuau cgcauuaaaa aauauaguaa agagaagagg aauauccuac      360

uuagaugacg cauccgaaga uggaggaacu gugucaucag acuauggaaa agcaguagaa      420

gagaaccgga aacuccucgc agagcaaacg ccuggccaaa uacaacuuga aagauucgaa      480

aaauacgguc aaguacgggg ggauuucaca guaguugaaa acggagaaaa aagacgacua      540

auaaauguau uuagcacuag ugcauauagg aaagaagcag agagaauacu cagaaaacaa      600

caagaauuca acaguaaaau uacugaugaa uuuauugagg acugccuuaa aauauuaacu      660

ggaaaacgaa aauauuacca uggucccgga aacgaaaaaa gcagaaccga cuacggaaga      720

uuucgaacug acggaacaac cuuagauaac aucuuuggaa uacucauugg uaaguguacg      780

uucuacccag augaauauag agcuagcaaa gccuccuaca ccgcacagga auucaaucua      840

uuaaacgacu uaaacaaccu aacuguacca acugaaacga aaaaacuauc agaagaacaa      900

aaaaaaguaa ucguugaaua cgcuaaagaa gcaaaaacac uaggagcgag uacacuauua      960

aaauacauug caaaaaugau cgacgccuca guagaucaga uccgaggaua ccgcgucgac     1020

guaaacaaca aaccagaaau acauacguuc gaagccuaca gaaaaaugca aucguuggag     1080

acaauaucag uaggagaacu aucccgaaac guucucgaug aguuagcuca uauuuuaaca     1140

cuaaauaccg aaagagaggg uauagaagaa gcuauuaaca cgaaauuaaa agacagcuuc     1200

agccaagauc aaguccugga auuaguucaa uuccgaaaaa acaacucauc acucuuuuca     1260

aaaggauggc acaacuucuc acuaaaacuc augauggaau uaauaccaga acuauacgaa     1320

acaagugaag aacaaaugac cauacuaaca agacuaggaa aacaaaaaag caaagaaaua     1380

agcaaacgaa caaaauauau ugaugagaag gaauuaacgg aagaaauaua caaucccgua     1440

guagcaaaau caguuagaca agcaauaaaa auaaucaacg aagccacaaa aaaacacggu     1500

auauuugaua auauagugau agaaauggcu cgggaaaaua augaagagga ugcaaaaaaa     1560

gauuauauaa aaagacagaa agcaaaccaa gaugaaaaaa acgcugcuau ggaaaaagca     1620

gcuuuucaau auaauggcaa aaaggaacuu cccgauagca uauuucaugg gcaaaaagaa     1680

cuugcaacaa aaaucagauu auggcaucaa caaggagaaa aaugucuaua uacaggaaag     1740

aacaucccaa ucagugacuu aauacacaau caguacaaau augaaaucga ccauauucuu     1800

ccacuauccc uaucguucga cgacagcuua agcaacaagg uccuagucuu agcaacugcc     1860

aaucaagaaa aaggacaaag aaccccauuu caagcacuag auaguaugga ugaugcaugg     1920

ucuuacaggg aauucaaaag uuauguaaaa gaaaguaagu uacuugguaa uaaaaaaaaa     1980

gaauauuuac uaacagaaga agacauaagu aaaauagaag uuaagcaaaa auuuauagaa     2040

cgaaacuuga uagacacacg cuacagcucg agaguaguuc uuaaugcacu gcaagacuuu     2100

uacaaagcgc auaaauuuga uacaacaauu aguguaguca gaggacaauu uacaagccaa     2160

cuacgaagaa aaugggguuu agaaaaauca agagaaacau aucaucauca cgccgucgau     2220

gcucucauaa uugcagcguc aucacaacuc agacucugga aaaaacaaaa caacccacua     2280

auagcauaua aagaaggcca auuuguagac agcgagacag gggaaauaau aucauuauca     2340

gacgacgaau acaaggaacu aguauuuaaa gcaccauaug aucacuuugu agacacauua     2400

aguucgaaaa cauuugaaga cuccauacua uucucguacc aaguugauuc uaaauuuaau     2460

cgaaaaauau cagacgcaac cauauacgcg acacgaaaag caaaauuaga caaagaaaaa     2520

agcgaggaaa cauacguucu aggcaaaaua aaagacauau acagccaggc aggguaugac     2580

gcauuuauaa aaauauauaa uaaagacaaa agcaaauucu uaauguauca caaagacccc     2640

caaacauucg aaaaagucau agaagaaauu cucagaacau acccaucuaa agaguuaaau     2700

gacaaaaaca aagaaauacc uuguaaucca uucgaaaagu acagacaaga aaacggaccc     2760

aucaggaaau auucaaaaaa aucaaauggc cccgaaauca aaucccucaa auacuaugau     2820

aauaaauuag gaaaccacau agauauaaca ccugaaaguu cagacaauca aguaguacua     2880

caaucauuaa aaccauggag aacagauguc uauuucaacc accaaacaaa aaaauaugaa     2940

cuuaugggcu uaaaguauuc agacuuaucu uuugagaagg ggagugguaa auauagcauc     3000

ucaaaccaaa aauacaauuc cauaaaacgc aucgaaggca uagaugaaca guccgaauuc     3060

aaguuuacac uauauaaaaa ugauuuaaua uuaaucaaag acagcgaaaa uaaugaacaa     3120

aaacucuucc gauuuaauag ccguaacgau acugcaaaac auuacaucga acuaaaaccu     3180

uacgauaaag caaaauucga cggcgaacaa gaauuaauua caauuuuagg aaacguagcg     3240

aaaggaggcc ggugcuuaaa gggguuaaac aaaucaaaca uauccaucuu uaaaguaaaa     3300

acagacgucc ugggaaaaaa acauauaaua aaaaaagagg gagacgaacc aaagcucaaa     3360

uuuuga                                                                3366


<210>  5106
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5106
augaacggac uaguguuagg ucuugacaua ggaaucgccu cuguaggugu cgguauucuu       60

aacaaagaca caggggaaau cauacacacc aauagccgua uuuuuccggc agcaacagca      120

gauaacaaug ucgaacgacg aaacucaaga caagacagaa gacuaaacag aaggaaaaaa      180

cauagaggag uccgacuaca ugaucuuuuc gaagacuaug aucugcucac cgauuuuuca      240

aaaguaucaa uuaaccuuaa ucccuaucga cuacgaguac aaggacuuga uaauucgcug      300

acaaccgaag aacuuuucau cgcacuaaaa aauauaguga aaagacgagg aaucucauau      360

uuagacgacg cuagcgagga cgguggagua guaagcucag auuacggcaa agcaguagag      420

gaaaacagaa aacuuuuagc agaacaaaca ccuggucaga uacaacuaga aagauuuaag      480

aaauaugguc aaguuagagg cgauuucaca guuguagaaa acggagagaa acgacgacua      540

aucaacguau ucucaaccag ugcauaccgc aaagaagcag aaagaauccu aagaaagcaa      600

caagaauuca auaaucaaau cacagauaaa uucauugagg acuaccuaaa gauuuugacg      660

gaaaaaagga aauacuacca cgggcccggc aaugaaaaau caagaacgga uuacggcaga      720

uuucguacag acgggaccac auuagacaau aucuucggga uccuaauagg aaagugcaca      780

uuuuauccga acgaguauag agcaaguaaa gcauccuaca cagcacaaga auucaaccua      840

uuaaaugacc uaaacaaccu uacgguacca acagaaacca agaaacuauc agaagaacaa      900

aaaaaaacaa uaguggaaua ugcaaagagc gccaaaacgc uaggagcauc aacccuacuc      960

aaauacauug cuaaaaugau cgacgcaucc guagaucaaa uaaggggaua ccgagucgac     1020

guaaauaaca aaccagaaau gcacaccuuu gaaguuuacc gcaaaaugca aucuuuggaa     1080

acuauaagug uaugggaguu aucacguaau auacuagacg aauuagcaca uauauuaaca     1140

cuaaacaccg aaagagaagg cauagaagaa gcaauaaaca caaaacuuau ggacgucuuu     1200

ucacaagauc aaguauuaga guugguacaa uuuagaaaaa acaacucaag ccuuuucagu     1260

aaaggauggc auaacuucag cagaaaacua augauggagc ucauaccaga auuauacgaa     1320

acaagcgaag aacaaaugac aauuuuaaca agacuuggca aacaaaaauc caaagagaca     1380

ucaaagagaa cgaaauauau agacgaaaaa gaaguaacag aagaaaucua caacccugua     1440

guagcuaaau caguaagaca agcuauaaaa auuauaaacg aagcaacaaa aaaauacgga     1500

auuuucgaua acauugucau agaaauggcc cgagaaaaca acgaagaaga ugcaaaaaaa     1560

gacuacauaa aaagacaaaa agcaaaucaa gaugaaaaaa acguugcaau agaaaaagca     1620

gcauuucaau auaacggcaa aaaagaacua cccgauucaa ucuuccaugg acacaaagaa     1680

cuagcuacaa aaauaaggcu uuggcaccaa caaggagaaa aaugcuuaua uacaggaaaa     1740

aacaucccaa uaucagaccu aauucauaau caauauaagu acgaaauaga ucacauacua     1800

ccccuaucgu uaaguuuuga cgauucacua uccaauaaag uauuaguauu agcaaccgca     1860

aaucaagaaa aaggacagag aacuccauuu caagcauuag auaguaugga cgacgccugg     1920

agcuauagag aauucaaauc auauguaaaa gacaguaaac uauuaggcaa caaaaaaaaa     1980

gacuaccucc uaacagaaga agacaucagu aaaaucgaag uaaaacaaaa auucauugaa     2040

agaaacuuag uagauacacg uuauucaucc agaguaguac ugaauacacu acaagacuuc     2100

uacaaagcac auaaauuuga uacgacaauc agcguuguaa ggggucaauu cacuucacag     2160

uuaagacgca aauggggccu cgaaaaaucc agagaaacuu aucaccauca ugccguagau     2220

gcauugauaa uugcagcaag cagccaauua agguuaugga aaaaacaagc caacccuuug     2280

auuuccuaca aagaggauca auucguagac agugaaacag gagaaauaau aagccuaacu     2340

gaugacaagu auaaagaacu aguauucaaa gcaccauacg cacacuuugu cgacacucug     2400

uccucaaaaa aauucgaaga uucaauccuc uucucuuauc agguagacuc gaaauauaau     2460

agaaaaauuu cugaugcuac aauauaugca acaagaaaag ucaaacuaga caaagauaaa     2520

agcgaagaaa cuuacguacu uggcaaaaua aaagauauuu auagucaagc uggauaugac     2580

gcguucauua aaauauacaa uaaagauaaa ucaaaauucc uaauguauca caaagaccca     2640

caaacuuuug agaaaguaau agaagaaauc cugcgaauau aucccucaaa agaguucaac     2700

gaaaaaaaca aagaaguacc guguaacccg uucgaaaaau acagacaaga aaacggacca     2760

auauguaaau acagcaaaaa agggaaugga ccagaaauaa aaagcuuaaa auauuacgau     2820

aaaaacauuc cagagucauu uauagacaua acaccaucaa auucaaagaa uaaaguuaua     2880

cuacgacaac ucaacccuug gcgaacagac guuuacuuca aucaccaaac ugaaaaauac     2940

gaauuacuag gacucaaaua ugcagaccua caauuugaaa agaaaacaga aacauauagu     3000

auaaccaucg auaaauauaa ugaaauuaaa gaaaaagaag gaguaggaga acacucugaa     3060

uucaaauuua cacuauacaa aaacgacuua cuacucauaa aagacaugga aauaaaagaa     3120

gaacaaauau uucgauuccu uaguagaaca augccaaaca aaaaacauua cgucgaacug     3180

aaaccuuauu acaaggguaa cuucgaaggc aaagaaagaa uaaaagucuu caauacaaca     3240

aguaacggaa gaugcuugaa aggguuaggc aaaaaaggcc uauccauaua uaaaauuaaa     3300

acggacguuc uuggaaauaa acauuucaua aagaaagagg gagaaaaacc aaaacuuaaa     3360

uucuga                                                                3366


<210>  5107
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5107
auggauagaa cagaagucaa caucggauuc gacauaggua ucucaagcgu aggaugguca       60

gucguagcaa cuaaaucagg aaggaucuua gaaagcggag ugucacuguu cucaggagcu      120

agugcagcua aaaacgaaga acgaagaaac uucaggcaaa gcaggagauu acuuaggaga      180

cgaaagaaca gaauaucaga uuuaaagaaa uauuuaaaag agcaaaguuu uacauauaac      240

ggugaauugc uaaacgaaaa cccguacgaa auucgaguaa aaggacucac agaaaaacua      300

ucaaaagaag aaauugcacu agcauuacuu cacauaguca aaaggagagg aguauccuau      360

gaucugggcg acauugaaga ggaaggaaca acagggucau acaaagaagg aguaaagaaa      420

aaucaaaaau uacugaaaga gaaaacaccg gcagaaauuc aauuccaaag auuaaagaaa      480

uuccacaaag ucagaggaca aauagcaaua gacgaaaaug auguccucuu gaauguauuc      540

ccuaaccacg cauacgugga cgaagcccaa caaauacugu acacacaaca agaauuuuau      600

ccagaaauca augaaacauu cauaaaccaa guuacagauu ucaucucaag aaaaagggac      660

uacuucauag ggccaggcuc ggagaaaagu cgaacugauu acggaauuua uagaacagac      720

ggaacaaccc uacaaaauuu auucagcauc cuaauaggaa aagacaaaau cuuucccgac      780

gaauuuagag cugccgggaa uaguuauaca gcccaaguau auaaucuacu caaugaccua      840

aacaaccuag cuauugauga cacagaaaca ggaaaauuaa ccaaacaaca aaaagagaaa      900

auaauaaugg aauuaaaaac uacagaaaaa ucagugaaua ugaugaaacu cauagcgaaa      960

acagccggcg uaacagucga acaaaucagc agauaucgaa uaaaccggga cgguaaaagu     1020

gaaauacaca acauggcagu cuacagaaaa aucagaaaag uauucuuaga acuaggcuac     1080

gaaaucaaug aauggccaag ucagcuacuu gacgaauugg gagacgcuuu aagccuaaac     1140

acugaaaacg gagaaaucag aagaauauua acagauaacu uagccaacaa auacaaaauu     1200

uuagaccaac agcucauuaa cuuaauuaua gaaaacaaaa acgcauucac cuuaagcaca     1260

aaccaaaaau ggcacagauu uucauacaaa acaaugaaaa uacuaauacc agaacuacua     1320

gaaacaaaca aagaacaaau gacgauacua acagaaaugg gacuuaugaa gaaagaccag     1380

agaaacuaug uagggaaaaa uaaaauagau ucagaagccc ugcuagaaaa ucuguacaau     1440

ccggucguuc ggaaauccac uagacaaacc augaaauuau uuaacacacu acuaaagaaa     1500

uacccaaaua uugcauaugu cguuauagaa augcccagag aagacgacga agauaaagca     1560

agaagagacu auguuaaauu ucaaaaagac aacgaaaaag aaaaagaugc agcccugaaa     1620

aacuuuauua uggaauccgg aaaaucagaa gcagaaguua uagcaaaaua ccgacaaagc     1680

aagaaauuua gaaguaaagu ccgauuaugg uaucaacaaa ugggaagaug ucuauauuca     1740

ggaaaggcaa ugccugccga aagaguauuu agagacgacc acuuuaccga uauagaccau     1800

guaauaccac ucucaguuuc auucgacgau ggaaugaaca auaaaguuuu gugcuuagcc     1860

gaaaugaaca gacaaaaaga uaagcaaacu ccauaugcuu ucuucaacgc aggucaugga     1920

cagggauucc aagcaaugaa ugcaaugcua aagaauaaca auagaaucuc cucggcaaag     1980

aaaagaaacu uauuauucac cgaaaacuua aacgaccuag aaguccgaaa aagauuuauc     2040

gaacguaauc uugucgauac aagauacgcu ucacgcgucg uauugaauga auuacaacaa     2100

uucguuaaag cuaaacaauu agauacaaaa guaaccguaa uaagaggaaa auucacauca     2160

aaacugagag aaaaauggaa uauaaauaaa agucgagaca cccaccacca ucacgcaguc     2220

gacgcugcug ucauagcagu gucaccaaug uuaaacuuau gggaaaaagg agccucaaua     2280

auaccaaaca aaguaggaga aaacguaauc gacauaaaga cuggagaaau uaucacagac     2340

caugauuacg aagcagaugu auacgagcuu cccuacgcaa aauuucuaga acaaauacca     2400

aaaguaaaga aagauauaaa auucagacac caaguagaua aaaagaaaaa ucgcaaagua     2460

ucagacgcaa ccauuuacac aacuagaaaa gcaaaagucg gaaaagacaa agaggaaaca     2520

gauuacgucc ugggaaccau aaaagacauu uauucacugg aagguuacgu uuccuucaag     2580

aaaauauaca acaaagauaa agcaaaauuc cugauggcaa gaauagaccc aaaaaccuuu     2640

gaaaaacuag agaaaauagu agcugaauau ccagaaucaa uugaaguugu acaagauaau     2700

ggaaaaguag auguccagaa aauaucacca uuugaauuau acaggaaaga caauggguuu     2760

guaacuaaau uugcaaagaa aaacaacggc cccgcuguua aaucguuaaa auacuacgau     2820

agcaaacuag gaagcucuau agauguaacu ccagauaaug cuaaaaauaa gaaaguaauc     2880

cuacaaucau ugaaaccaug gcgaacagac gucuacuuca acgaagaaaa ccaagaauac     2940

gaaauaaugg gucuaaaaua uucagaucua aaauuuggua aaaacggaaa auacggagua     3000

ccaaaaguaa gauaugaaga aauuaaacaa gaagaagaaa uaucagaagg uuccaaauuu     3060

cuauucucac uguaccgcgg agacaggaua aaggucacca acgaacuagg agaaaaccuu     3120

gaucuauugu uuucaagcag aacgcuacca gcacaaaaag gguauguuga auuaaaacca     3180

aucgauaaaa caaaguucga ugguaaagaa aaggucucau ucuacgggac uguaaccccu     3240

aauggacaau ucguaaagaa auucgcaaag aaaggauaca aaauucucaa acucaacaca     3300

gaugaauuag gaaauacaua uauagaaaac ggagaaaaag agcccagcga cauacuugau     3360

auguga                                                                3366


<210>  5108
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5108
augaacggau uaguauuagg auuagacaua ggaauaggaa gcguaggcgu cggcauucua       60

aacaaagaca caggagaaau uauccaugcg aacucaagaa uuuucccugc ugcuacagca      120

gacaacaaug uaguaagaag aucaagucgu cagggacgga gauuaaacca cagaaagaaa      180

caucgaagug uacgacuaaa agauuuauuu gaauauuaug aucuacugac agacuucucc      240

aaagucagua uuaaccuaaa uccauauaga cuaagaguaa acggccuuaa ucaacaauua      300

acgaaugaag aacuuuuuau cgcacucaaa aacauaguca aacgaagggg aaucucuuau      360

cucgacgacg caagcgaaga uggaggagca guuucgucag auuauggcaa agcaguagaa      420

gaaaacagaa aacuucucgc ugagcaaaca ccaggccaaa uacaauuaga gagauuugag      480

aaauacggcc agguucgagg cgauuucaca guagucgaaa auggagagaa aagaagacuc      540

auaaauguau ucagcacaag ugcauauaag aaagaagcag aaaggauauu aagaaaacaa      600

caagaauaca auaaucaaau aauggacgag uucauagcag auuauuuaac uauacucacc      660

ggaaaaagaa aauauuacca cgguccagga aacgaaaaau ccaggacaga uuauggacgu      720

uucaggacag auggaaccac auuagauaau auauuuggaa uccugaucgg aaaaugcaca      780

uucuacccag aaaaauacag agcaaguaaa gcaucuuaca ccgcacaaga guucaaccua      840

cuaaacgauc uaaacaacuu aacgguaccc accgaaacca agaaauuauc cgaagaacaa      900

aagaaaacua uagucgaaua ugccaaaucc gcaaaaacac ucggugcauc aacucuucuu      960

aaauacauag caaaaaugau agaugccuca guugaucaaa uacgaggcua cagaguagau     1020

gucaaaaaca aaccagaaau gcacaccuuc gaaguauacc gaaaaaugca aucacuagaa     1080

acaauaucag uaggggaauu uucaagagaa guauuagacg aacuagcaca uguacucaca     1140

cuaaacacag aaagagaagg aauagaagaa gcaauaaaua caaaacucag agauagcuuu     1200

ucgcaagacc aaauucuaga gcuaguacag uuucggaaaa auaauucaag ccuauuuuca     1260

aaaggauggc acaacuucag cuuaaaacua augauggaau uaauacccga acuauacgaa     1320

acgucagaag aacaaauggc uauacuaaca agacuaggaa aacagaaauu caaagaaaca     1380

ucaaaaagga ccaaauauau agacgaacaa gaaguaacag aagaaaucua uaacccggua     1440

guggcgaaau cugucagaca agccauuaaa auaaucaaug aagcaacaaa gaaauacgga     1500

auauucgaca auauagugau agaaauggcc agagaaaaua acgaagaaga agcaaagaaa     1560

gacuauauaa aacgacaaaa agcgaaccac gacgagaaaa augcagccau ggaaaaagca     1620

gcauuucaau acaacgguga aaaagaacua ccggauagua uauuucacgg acauaaagaa     1680

cuagcgacca aaguacgacu uuggcaucaa caaggcgaaa aaugccuaua uacaggcaaa     1740

aauaucccua uaagcgaccu aauacacaau caguauaaau acgaaaucga ucauaucuug     1800

ccacuaucuc uuuccuucga cgauucacua ucaaauaaag uccuuguucu agcaacagca     1860

aaccaagaaa aaggacaaag aacaccauuu caagcacuag acucaaugga ugaugcuugg     1920

ucauaccgcg aguucaaauc uuacguuaaa gacaguaaau cccucggaaa uaaaaagaaa     1980

gacuauuuau uaacagaaga agacauaucc aaaauagaag ugaaacaaaa auuuauugaa     2040

agaaaccuag uugacacaag guacagcuca cgaguaguac uaaauacucu ucaagacuuc     2100

uacaaagcac acaaauucga caccacaaua ucaguaguac gaggacaauu uacgagccag     2160

cuuagaagga aauggggacu agaaaaaucu agagaaacuu accaucacca cgcaguugac     2220

gcacuaauca uagcagcgag uagucaauua cgacuaugga agaaacaaaa caauccucua     2280

auaucauaua aagaaggcca auuuguagac ucagaaacgg gagaaaucau aucacuaacc     2340

gacgacgaau auaaagaacu cguauucaaa gcucccuacg accauuucgu agacacacuc     2400

agaucaaaga aauuugaaga cucaauccug uuuucguacc aaguagacuc aaaauuuaac     2460

cgaaaaauau cagacgcaac aauuuaugca acccgcaaaa caaaacuuga caaagacaaa     2520

ucugaagaaa cuuauguacu uggaaagaua aaagacauau acagccaauc uggauacgau     2580

gcauuuauaa aaauauacaa caaagacaag ucaaaauucc uuauguacca uaaagaccca     2640

cagacauucg aaaaaguaau agaagagauc cuaagaacuu accccucaaa agaguucaau     2700

gagaaaaaua aggaaguauc augcaaucca uuugaaaaau auaaacaaga aaauggcccc     2760

auaagaaaau acuccaagaa aggaaacgga ccagaaauaa aaagccucaa auacuacgau     2820

aaaaauaucc ccgagagcuu cauaaauauc acaccaucaa acuccaaaaa caaaguaauc     2880

uuaaggcaac ucaacccaug gcgaacugac guauacuuca accaucaaac agaaaaauac     2940

gaacuauugg gucuaaaaua cgcugaucua caguucgaaa agaaaacagg aacuuacucc     3000

auaacgauag acaaauauaa cgaaauuaaa gaaaaagaag guguaucaga acacagcgaa     3060

uuuaaauuca cccuauauaa aaaugaccuc cuacugauca aagacacaga gauuaaggag     3120

gaacaaauuu uuagauuuuu aucgcgaacc augccaaauc aaaaacauua cguagaacua     3180

aaacccuauu auaaaggaaa cuucgaagga aaagaaagaa uaaaagucuu uaacaccaca     3240

ucaaacggua gaugccuuaa agguuuacga aagaaaggcc uaucuauaua caaaauaaaa     3300

acagauguac uaggcaacaa acacuucaua aagaaagaag gcgaaaaacc gaaauugaaa     3360

uuuuga                                                                3366


<210>  5109
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5109
augagcgacu uaguacuagg acuugacaua gguaucggau cuguaggagu uggcauacuu       60

aacaaaguaa ccggagaaau aauucacaaa aacucgcgaa uauucccugc ugcccaagca      120

gaaaauaauu uaguacgccg uacaaaccga caaggucgua gauuaacucg aagaaagaaa      180

cauaggauag uccgccuaaa ucgccuguuc gaagaaagcg ggcuaauaac cgacuucaca      240

aaaauuucaa uuaacuuaaa cccauaucaa uuaaggguaa aaggccuuac ugaugaacua      300

ucaaacgaag aacuauuuau agcacuuaaa aacaugguaa aacacagagg aaucucauac      360

uuagacgaug caucugauga cggcaauucu uccguuggag acuaugccca aauuguaaaa      420

gaaaacagca aacaacuaga aaccaaaaca ccaggucaaa uacagcucga aagauaccaa      480

acauacggac aguugagagg agacuuuaca guagaaaaag acggaaagaa acauagacua      540

auaaacguuu uccccacauc ggcguauaga uccgaagcau uaagaauauu acaaacacaa      600

caagaguuca auccacaaau aacagaugag uucaucaaca gauauuugga aauauuaaca      660

gguaaaagaa aauacuauca uggaccaggu aacgaaaaau cacgaacaga cuauggcaga      720

uauagaacca gcggagaaac ccuagacaac auauuuggaa uccucauagg aaaaugcacc      780

uucuauccug acgaguuccg cgccgccaaa gccaguuaua cugcacaaga guucaaccua      840

cuaaaugacc uaaacaacuu aacuguacca accgaaacca agaaacuauc aaaagaacag      900

aaaaaucaaa uaaucaauua cgugaaaaau gaaaaagcaa ugggacccgc caaacuauuc      960

aaauauauag cuaaacuccu gaguugcgau guagccgaua uaaaagaaua uagaaucgac     1020

aaaucuggua aagccgaaau acauacuuuc gaagcauauc gaaagaugaa gacauuagaa     1080

acccuugaua uugaacaaau ggacagagaa acgcuagaca aguuagcaua cguauuaacu     1140

uuaaacaccg aacgagaagg uauacaagaa gcauuagaac acgaauuugc cgacggcucu     1200

uuuagucaaa aacaaguaga ugagcuagua caauucagaa aagcuaacag uaguauauuu     1260

gggaaaggau ggcacaauuu caguguuaaa cuuaugaugg aacuuauccc cgaacuauac     1320

gaaaccucag aagaacaaau gacuauauua acaagacuag gaaaacagaa aacaacaagc     1380

ucauccaaca aaacaaagua uauagaugaa aaauuacuaa cagaagaaau auauaauccc     1440

guaguggcaa aaucuguccg acaagcuaua aagauaguaa acgcugcaau aaaagaauac     1500

ggugacuuug acaacauagu caucgaaaug gcuagagaaa ccaaugaaga cgaugaaaag     1560

aaagccauac agaaaauaca aaaagcaaau aaagaugaaa aagaugcagc aaugcuaaaa     1620

gcagccaauc aauacaaugg aaaagcugaa uuaccacacu cagucuucca cggccauaaa     1680

caauuggcaa caaaaauaag acuauggcac caacaaggcg aaagaugucu auacacggga     1740

aaaacaauua guauacauga ccugauaaac aauaguaauc aauucgaagu ugaccauauc     1800

cucccgcugu caauuacguu cgaugauagu uuagccaaca agguauuggu auaugcuaca     1860

gccaaccaag aaaagggcca aagaacacca uaucaagcgc uagauagcau ggacgacgca     1920

uggagcuuca gagaacuaaa ggcuuucgua cgggaaucua aaacacuauc aaauaaaaag     1980

aaagaauauc uauuaaccga agaagacaua ucaaaauucg acguaagaaa gaaauuuaua     2040

gaaagaaauc uaguggauac aagauacgcu agcagagucg uccuaaaugc auuacaggaa     2100

cacuuuagag cacacaaaau agacacaaaa gucuccguag uuagaggaca auucacaagc     2160

caacuaagga gacacugggg cauagagaaa acaagagaca cauaccauca ucacgccgua     2220

gacgcauuaa uaauugcugc aucaauacaa cucaauuugu ggaagaaaca gaaaaauacc     2280

cuagucuccu auagcgaaga ccaacuauua gauauagaaa caggagaauu aauaucagac     2340

gaugaauaca aagaaagugu uuucaaagcc ccauaucaac acuucguaga cacuuuaaaa     2400

ucaaaagagu ucgaagacuc gauacuauuu ucauaucaag uggacaguaa auucaaucga     2460

aaaauaucag acgcaacgau cuacgcaaca cgccaagcaa aaguggguaa agacaaagcc     2520

gacgaaacuu acguauuagg aaaaauaaaa gauauauaca cucaagacgg auaugaugcg     2580

uuuaugaaaa uuuacaagaa agauaaaucu aaauuccuga uguaccgcca ugacccgcaa     2640

acauucgaaa aaguaaucga acccauauua gaaaauuauc caaauaaaca aauaaacgaa     2700

aaagggaaag aagucccagu caauccauuc uuaaaauaua aagaggaaca uggauauaua     2760

cgaaaauacu caaagaaagg uaacggacca gaaaucaaau cgcucaaaua uuacgacucc     2820

aaacuuggaa accauauaga cauaacuccc aaagauucua acaacaaagu aguacuacaa     2880

ucugucucac cauggcgagc agacgucuac uuuaacaaaa caacaggaaa auacgaaaua     2940

cuuggacuua aauacgcaga ccuacaauuc gaaaaaggaa caggaacaua uaaaauaagc     3000

caagaaaaau acaaugacau uaaaaagaaa gaaggaguag acucagauuc ugaauuuaaa     3060

uucacacuau acaaaaacga cuugcuacuc gucaaagaca cagaaaccaa agaacaacaa     3120

cuauuccgau uccuaucaag aacaaugcca aaacaaaaac auuacguaga auuaaaaccg     3180

uacgacaaac aaaaauucga agggggagag gcacuaauca aaguucuagg uaaugucgcc     3240

aauucuggac aaugcaagaa aggacucgga aaaagcaaca uuagcauaua caaaguccgu     3300

acagauguau uagguaauca acacauaauu aaaaaugaag gagauaaacc aaaauuagac     3360

uuuuga                                                                3366


<210>  5110
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5110
augaacgguc uagucuuagg acucgacaua ggaauagcca guguaggagu cggaauauua       60

gagaaaaaua gcggaaaaau aauacacgca aauucucgcc ucuucccagc agcaacagca      120

gacaauaacg uagaacgucg gaaaaacaga caagcuagaa gauuaaaccg aagaaagaaa      180

cauaggggag uccgauucca agaccuauuc gaagacuaug gucuacuaac agacuuuuca      240

aaagugagca uaaacuuaaa uccguauagg uuaagaguag acggccuaaa ucaacaauua      300

acaaacgaag aacuauucau cgcucugaaa aauauuguca aacguagggg aauaaguuau      360

uuagaugaug cauccgaaga cggaggcgcc guaucaucag auuacgguaa agcuguagaa      420

gaaaaucgaa aacuacucgc agaacaaacc ccaggacaaa uacaacuaga aagauucgaa      480

aaauauggcc aaguaagggg agauuuuaca guaguagaaa auggagaaaa augccgccuu      540

aucaaugucu ucaguacuuc ugcuuaccga aaagaggcag agaaaauacu uaggaaacaa      600

caagaguuca acaaccaaau aaccgacgaa uuuauugaag auuaccuaaa aauauuaacu      660

ggcaaacgua aauacuauca uggaccagga aacgaaaaau caaggacaga uuacggaaga      720

uuucggacag auggaacaac ccucgacaac auauucggcg uacuaauagg uaaauguaca      780

uuuuauccaa augaauacag agccagcaaa ucgucauaca ccgcccaaga guucaaccua      840

cuuaaugacc ucaauaaucu caccguacca acggaaacaa agaaauuaag ugaagaacaa      900

aagaaaguaa uaguugaaua cgcuaaagaa gccaaaacau uaggagcguc aacacuguua      960

aaauacauag uuaaaaugau agacgcuucu gucgaucaaa uuucuggaua uaggguagau     1020

guaaacaaca aacccgaaau gcacaccuuc gaagucuaua ggaaaaugca aucucuagaa     1080

acuaucagcg uaggagaauu aucaagaaac guauuagacg aacuagcaca uauacuaaca     1140

cuaaauacag aaagggaagg caucgaagaa gcaauaaauu cuaaauuaau gggaguauuu     1200

agccaagacc aaguuuuaga auuaguacaa uuccgcaaaa acaacucauc acucuuuagc     1260

aaagguuggc auaacuucag ccuaaaauua augauggaau uaauaccuga acuuuacgaa     1320

acaucagaag aacaaaugac cauacuaacu agacuaggca aacaaaagag uaaagaaaca     1380

aguaaaagaa caaaauacau agaugaaaaa gaauuaaccg aagaaauaua uaacccggua     1440

guugcuaaaa gugucagaca agcaaucaaa aucauaaacg aagcaacuaa gaaacacgga     1500

auauuugaua acauagucau agaaauggcg agagaaaaca acgaagaaga cgcaaagaaa     1560

gacuacauaa aacgacaaaa agcaaaccaa gacgagaaaa acgcggcgau ggaaaaagca     1620

gcauuccaau auaacggcaa gaaagaacua ccaggcaaca uauuuaacgg ccacaaagaa     1680

cuagcuacaa aaauacgcuu guggcaucaa caaggggaaa aauguuugua cacaggaaaa     1740

acaauaccaa uaagcgacuu aauccacaac caauacaaau augaaaucga ccacauccua     1800

ccccuuucac uuagcuuuga cgacucauua ucaaauaaag ucuuaguuca ugcaacagca     1860

aaucaagaaa aaggacaaag aacuccauuu caagcacuag acucaaugga cgacgccugg     1920

agcuacagag aauuuaaaag uuauguaaaa gacuuaaaau cguuauccaa caaaaagaaa     1980

gauuaccuac uuaccgagga agacaucuca aaaaucgaag uaaaacaaaa auuuauagaa     2040

agaaaucuag uugauacaag auacagcucu agaguugugc ugaacgcucu acaagacuuc     2100

uacaaaagcc accaauucga cacaaccaua agcgucguaa ggggccaauu uacaucacaa     2160

cucagaagaa aauggggacu ggagaaaagu agggaaacau aucaucauca cgcuguugau     2220

gcauuaauca ucgcagcauc cucacaacug agguuaugga agaaacaaaa uaaucccuua     2280

auaacauaca aagaaggcca auuugucgau ucccaaacag gagaaauuau uagcuuaaca     2340

gacgaugaau acaaagaacu cguauuuaaa gcaccuuaug accauuucgu agacacacua     2400

cacucuaaaa cauuugaaga uggaauauua uuuagcuacc aaguagacuc aaaauacaac     2460

agaaaaaucu cggacgcuac aaucuacgca acaagaaaag ccaaacuaga caaagauaaa     2520

agugaagaaa cuuacguacu aggaaaaaua aaagacauau acucccaagc uggauacgau     2580

gccuuuauaa aaauauacaa caaagacaaa agaaaauucc uuauguacca uaaagaccca     2640

caaacuuucg aaaaaguuau cgaagaaauc uuaagaacau auccaucaaa agagagaaac     2700

gauaaaaaua aagaagugcc cuguaaucca uuugaaaaau aucgucaaga aaacggcccc     2760

aucagaaaau acucaaagaa aggaaauggg cccgaaauaa aaugccuaaa auauuaugac     2820

aacaaacucg gcaauuacau cgauauauca ccagacggau cugauaacca aguaguccua     2880

caaucacuca aacccuggag gaccgacguc uacuuuaacc aucaaacaaa agaauacgag     2940

cugaugggcc uuaaguauuc ugauuuaagu uucgaaaaag ggagcggcaa auacagcauu     3000

agcaaugaaa aauacaauag cauaaaaagg auagaggggg ucaaugaaca aucugaauuu     3060

aaauuuacuc uauacaaaaa cgaucuaauc uuaauaaaag auuccgaaaa caacgaacaa     3120

aagcuauuca gauucaacuc gagaaaugac acugcuaaac acuacguaga acugaaaccg     3180

uacgauaaag caagauucga uggacaacaa gaacuaauaa caauccucgg uaacguggca     3240

aaaggagguc aauguuuaaa aggauuaaac aaaucaaaca uaucuauuuu uaaaguaaag     3300

acggauguau uaggaaagaa acacaucauc aagaaagagg gggacgaacc caaacuuaaa     3360

uucuga                                                                3366


<210>  5111
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5111
auggcagaua aaaccuuagg agcacuaaca uucggauuug auauagguau agcaucaguc       60

ggcuggugug uguuaggaga aaacagaauc guugaccucg gaguucgagc uuuugauaaa      120

gcugaaacgg caaaagaagg ugaaagccuc aaucucgcua gaagaaccgc acgccuuaug      180

agacgcagac ucagaagacg agcauggaga cuaacgaaac uagcacgacu acuuaaaaga      240

gaaggauuaa uugcagaugc auuccaaaug caucaaccaa gugcgacauc uccguggaag      300

uuaagaguag aagcgcugga ccgccgccuc acagacgacg aaugggcaag aguuauauau      360

caucucugua aacaccgugg uuuucacugg auaagucgug cagaagaaaa gaaagcagaa      420

ggagaugcaa aaggugaauc aggucgugua aagaaaggau ugagugauac acaaagacga      480

augggagaaa aaggauaucg guccgcagcu gaaaugguac uugcugaauu uccugaagcu      540

caaaggaaca aacaaggaga uuauggcaaa gcauuaagca gaguacuauu aagugaagaa      600

uuagcauuau uguuuagaag acaaagagaa cuaggucaca cucaugcauc uaaagaacua      660

gaagcagcga ucuuaggaaa uggagaucga aaaggagguu uguuuugggc ucaaaaaccu      720

ccacuagcag gagcagaucu uuuaaaaaug cuuggcaaau guacuuucga aaaagacgaa      780

uacagagcac caaaagcauc uuuuaccgca gaaagacacg uaugguugac caggcucaau      840

aauuuaagag ucguaguaga ugguaccacg agaccauuga cagaagacga aagacgggua      900

gcacuaccau uaccauaugg cuaugcggac gcuaaauuaa cauauaaaca auuaagagcu      960

gcauuaagca aagcugguuu aaguaauuuu aguuucgcgg gaauugcuga ugacaaagaa     1020

aaagauacgc uaacaaaauu accagcaugg gaaacgcuau ccaaaacacu aaaaggaaaa     1080

ggcuuagaaa cugaauggga aggaauggcg ggugcagcua cgggaggaga cccgacuuua     1140

cuugaugaaa uaagcagagu acucucagua uauaaagaug gcgacgaagu ggacguugaa     1200

uuaagaaaau uaccucuacc cggaggagag aaaaugguug aaacauuaag cgaaauuagc     1260

uuugacaaau uucacgcauu aucguuaaaa gcacuacgaa aaauaguacc acauauggaa     1320

acuggacuca gguaugauga agccugcgca gcggcaggcu aucaucauag ccaacuccau     1380

gaagcaggcg cggguggaca aaaauauuua ccaccuuucu auacacgacg ggauaaagac     1440

gggagaaugg uauucgacga ggaacuagac guaccuagaa acccaguagu auuaagagca     1500

uugaaccaag caagaaaggu auugaacgcc cuaguaagag cauauggcuc uccaucagca     1560

gugcacauag aaauggcaag ggauuuaagu aaaccgcuag ugggacaucc uaccccgaau     1620

ggaggcuaua uagaagguag aagagauaua gaaaaacucc agaaagaaua uagagaaaga     1680

aaugaaaaag auaaagccgc auucgcagca gaacauggua uagcagcaaa aucaagagag     1740

uucgaaaagu uucaacuuua uagagaacaa caaggcaaau gugccuauag uuugaaaccc     1800

cuggaccuac aucgaguauu acaugacaua gguuaugcug aaguugacca cgccuuaccc     1860

uauucacgcu cauacgacga ugggaaaaau aacaaaguac uugucuugac agaagaaaau     1920

agaaacaaag ggaaucggac agcauacgaa uaucuaugug cuuuuccagg uggggaaaau     1980

ggagagagau ggagaaauuu cgucgcauau guagaaagua acaaaaguua ccgacuagcc     2040

aaaagaacaa gauuauuaag aaaagacuuc ggaggaaaag ccgcagagga auuucgagaa     2100

agaaacuuaa augauacgag auauauaugu aaauucuuua aaaauuuugu agagagguuu     2160

uuacaacuaa aggugggagc agauggaaca gcagcaaaaa gaugcguagu acucucagga     2220

caaacaacag cauuuuuaag agcuaggugg ggucuauuaa aagucagggg ugcuagugau     2280

cgacaucaug cacuugaugc agccguaguu gccgcuuguu cacauggaau gguaaaaaga     2340

uuagcagacu acagcagaac uaaggaacuc gcaaaaguac gagauggauu cccggaucca     2400

gaaaccggag aaauaguaga cccagcgaug uuucaacaac uacaugcaca cuuuccugac     2460

ccauggcccc auuucagagg ggaacuagaa gcacgacuac agcuagauga uucggcagaa     2520

uugcgcgcac aacuugaaau guuuggaaca uauccagcgg aagcacuaga aacauuaaga     2580

ccacuauuug uuucacgugc accacaaaga agaaauggcg gagcagcaca caaagaaacu     2640

auauacgcac aaccugaacg ccucaaggaa caaggagggg uaacacaaaa aguaucccua     2700

gccucccuaa aaccaacaga uauugauaaa uugaucgauc cacaccgaaa cgaaaaauug     2760

uacgcggcaa uacgcgcgag acuagaagcu caugguggaa aaucagauaa agcauucccu     2820

gcugauaauc cacuacgaaa accggacaga gaugguaauc cuaccggucc aauaguccgc     2880

gcuguaacaa ugguaauaga caagcugucg gguauacaaa uuagaggagg aauugcaaag     2940

aaugacacga ugcuacgagu ugacguauuu cgacacaaga aagauggcaa auuucauuua     3000

guaccacucu acguacacca cgcuguggcu caagaauuac cgaauagagc uauaguagcu     3060

uucaaagaug aagaugaaug gacacuaaua gaugauaacu uucacuuuuu auuuuccuuu     3120

cauccaaaug acuucauaag aaucacacag aaaaacaaag caccuauauu aggauauuau     3180

gcaucauguc accgaggaac uggcaaucua aaucuauggg cacaugaucg aaaucaaaaa     3240

guagguaaag auggacaaau ugaaggaauu gggguaaaga ccgcgguagc ucuagagaaa     3300

uuucacguag acguguuagg aaauauauau ccagcgcccc cagaacaacg acacggacua     3360

gcauga                                                                3366


<210>  5112
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5112
augucagauu uaguauuagg auuagacaua ggaauaggau cuguaggugu aggcauauua       60

aauaaaguua cuggagagau aauacauaaa aauagcagga uauuuccagc cgcacaagca      120

gaaaauaacc uuguucggag aacaaaccga caaggaagaa ggcuuacuag acguaagaaa      180

caccgcaggg ucaggcuaaa ccgcuuguuc gaagaaagcg gacuaauaac cgauuucaca      240

aaaauaucaa uuaaucuaaa uccauaucaa cuacgaguga aagguuuaac agaugagcua      300

ucaaaugaag aacucuucau agcccuaaag aacaugguca aacaccgcgg uauauccuau      360

cuagacgacg cgagugacga cggaaacuca ucaguaggcg auuacgcaca aauagugaaa      420

gaaaauucca aacaacucga aacaaagacc ccaggccaaa uacaacuaga acguuaucaa      480

acuuacggac aauuacgagg ugauuuuaca gucgaaaaag augguaagaa acaucggcuu      540

auuaauguau ucccaacaag ugccuaucga ucagaagcau uacgaauccu acaaacacaa      600

caagaauuua auccacaaau aacugaugag uucauaaaua gguauuugga aauacuaaca      660

ggcaaacgaa aauauuacca cggcccuggu aacgagaaaa gucgaacaga cuacggaaga      720

uacagaacaa guggagaaac ucuagauaac auauucggaa uauuaauagg aaaaugcacu      780

uucuauccag acgaguucag agcagcaaag gcuucauaua ccgcccaaga guucaauuua      840

cuaaacgacu uaaacaacuu aacaguaccg acagaaacaa agaaacucag uaaagaacag      900

aaaaaccaaa uaaucaauua cguuaaaaau gaaaaagcaa ugggaccagc aaaauuauuc      960

aaauacauag cuaaacucuu aucaugcgac guagcugaca uaaaaggcua uagaaucgau     1020

aaaaguggca aggccgaaau ucacaccuuc gaagcauaua gaaagaugaa aacacucgaa     1080

accuuggaca uagaacaaau ggauagggaa acacuagaua aacuugcaua uguauuaaca     1140

cuaaacaccg aaagggaagg aauacaagaa gcacuagaac acgaauuugc ggauggauca     1200

uucucacaaa aacaaguaga cgaacucgua caauuccgaa aagcaaacuc aagcauauuc     1260

gguaaaggau ggcauaauuu cucaguaaaa cugaugaugg aacuaauacc agaacuauac     1320

gaaaccucgg aagaacaaau gacgauacua acaagguuag gcaaacagaa aacgacauca     1380

ucaucaaaua aaacuaaaua cauagaugaa aaacuauuaa cagaagaaau auacaaccca     1440

gucguagcca aaaguguccg acaagcuaua aaaauuguua acgcagcuau aaaagaauau     1500

ggcgacuucg auaauauagu aaucgaaaug gcacgagaaa caaaugaaga cgacgaaaag     1560

aaagcgauac agaaaaucca aaaagcaaac aaagacgaaa aagaugcugc aaugcuaaaa     1620

gcggcuaauc aauauaacgg uaaagcugaa uuaccccacu caguauucca cggccauaaa     1680

caacuggcua caaaaauuag acucuggcac caacaaggag aacgaugucu auauacagga     1740

aaaaccaucu caauucacga ucucauaaac aauagcaauc aauucgaagu agaucauauu     1800

cuaccucucu cuaucaccuu ugaugacucc cuagcaaaca aaguacucgu auacgccaca     1860

gcaaaccaag aaaaaggaca aagaacaccc uaccaagcac uagacucaau ggacgacgca     1920

uggaguuuuc gugaacuuaa ggcauuugua agagaaagca agacauuauc uaacaaaaag     1980

aaagaauacc ucuugaccga agaagacaua ucaaaauucg augucagaaa gaaauuuauc     2040

gaacgaaauc uugucgacac gcgauaugcu ucaagaguag uacucaacgc cuuacaagag     2100

cauuuuagag cacauaaaau ugauacuaaa guaucaguag uccgaggaca auuuacaagc     2160

caauuacguc gacauugggg aauagagaaa accagagaca cauaccacca ucacgcugug     2220

gacgccuuaa uaauugccgc aucgagccaa uuaaacuuau ggaagaaaca gaaaaacaca     2280

cucgucagcu acucugaaga ucaacuacua gacaucgaaa caggcgaacu aauauccgau     2340

gaugaauaca aagaaucagu auucaaagcg cccuaccaac acuuuguaga uacauuaaaa     2400

ucaaaagaau uugaagacag uauucuauuc ucauaucaag uagauagcaa auuuaacaga     2460

aaaaucucag augcaaccau auacgcaaca agacaagcca aaguaggaaa agacaaagcu     2520

gaugagacau acguacuagg aaaaaucaaa gacauauaua cacaagacgg auacgaugca     2580

uucaugaaaa uauauaagaa agauaaauca aaauucuuaa uguaucgaca cgauccacaa     2640

accuuugaaa aaguaauaga acccauccua gaaaauuauc caaauaaaca aauaaaugaa     2700

aaagggaaag aaguacccug uaacccauuu uuaaaauaca aagaagaaca uggcuacaua     2760

cgaaaauacu cgaagaaagg aaacggaccc gagaucaaaa gccuaaaaua uuacgacagc     2820

aaacucggca aucacauaga uaucaccccc aaagacucca acaacaaagu aguacuacaa     2880

aguguaucuc cauggcgagc cgaugucuac uuuaauaaaa ccacaggaaa auaugaaaua     2940

cuaggccuaa aauacgccga ucuacaauuu gaaaaaggaa cuggaacaua uaaaauuagu     3000

caagaaaaau auaacgauau uaaaaagaaa gagggagucg acagugacag cgaauuuaaa     3060

uucacucuau auaaaaacga ucuacuauua guaaaagaua cggaaacgaa agaacaacaa     3120

cucuuccgau uucuaagcag aacaaugcca aaacaaaaac acuacguaga acucaaacca     3180

uacgacaaac aaaaauucga aggaggugaa gcacugauaa aaguauuagg uaauguagca     3240

aacagcggac aauguaaaaa gggcuuaggu aaaucaaaca uaucgauaua uaaagugaga     3300

acagauguac uaggaaauca acauauaauc aaaaacgagg gcgauaaacc uaaacucgac     3360

uucuga                                                                3366


<210>  5113
<211>  3366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5113
augaaugguc uaguauuagg acucgacauu ggaaucggau caguuggagu aggaauacua       60

aauaaagaua caggcgaaau aauucaugca aacucaagaa uauucccagc agcuaccgca      120

gauaacaacg uuguaagacg uucuucccga caagggagac gauuaaacca ucgaaagaaa      180

caucgauccg ugcgacuaaa agauuuauuc gaauauuaug accuacuuac ggauuucagu      240

aaaguaagua uuaaccuaaa ucccuaccgc cuccgcguag auggauuaaa ucaacaauua      300

acaaaugaag aauuauuuau agcacucaaa aacaucguua aaagaagggg caucagcuau      360

uuagaugaug ccagugaaga cgggggcgca guauccucag acuaugggaa agcagucgaa      420

gaaaaucgaa aauuacucgc ugaacagacu ccaggacaaa uucaauuaga acgauucgaa      480

aaauacgguc aagucagggg agauuuuaca gucguagaaa auggagagaa aaggcgauua      540

aucaaugucu uuucaacauc agcauauaag aaagaagccg aaagaauccu uagaaaacaa      600

caagaauaca auaaccaaau aauggaugaa uuuauagcag acuaccuuac aauacuaaca      660

ggaaaaagaa aauacuauca cggcccggga aacgaaaaau caaggacaga cuacggucga      720

uucagaaccg augguacuac acuagauaac aucuucggua uccucauagg gaaaugcaca      780

uuuuauccag aagaauaucg cgcaagcaaa gcaucauaua cagcacaaga auuuaaccua      840

uuaaacgauc uaaauaaucu uacagucccu acugaaacca agaaauuguc agaagaacaa      900

aagaaaacaa uagucgaaua cgcaaaaucu gcaaaaacac ucggagcaag cacccuacua      960

aaauacauag cuaaaaugau agacgcaucc guagaccaaa uaagagguua uaggguagau     1020

guuaacaaca aaccugaaau gcacacuuuu gaaguauaua gaaaaaugca acuauuagaa     1080

accauaccag ugggcgaauu uucaagggaa guccuagacg aauuagcaca cauauuaacc     1140

cucaacaccg aacgagaagg uauagaagaa gcaauaaaca ccaaacuaau ggacgucuuu     1200

ucacaagacc aaauccuaga auuaguacaa uuccguaaaa auaauaguuc guuguucagc     1260

aaaggcuggc acaacuucuc acuaaaacuu augauagaac uaauaccaga acuauacgaa     1320

acauccgaag aacaaauggc cauauuaaca agacucggua aacaaaaauu caaagaaacc     1380

uccaaaagaa caaaauauau agacgaacag gaaguaacag aagaaaucua caaccccguu     1440

guggccaaau cuguaagaca ggccauaaaa aucauuaaug aagccacaaa gaaauacgga     1500

auauuugaua acauuguaau agaaauggca agagaaaaua acgaagaaga agcuaagaaa     1560

gacuacauaa aaagacaaaa agcaaaucac gaugagaaaa acgccgcaau ggaaaaagcu     1620

gcauuccaau auaauggaga aaaagaacua ccugacucca ucuuucaugg acacaaagaa     1680

uuggcgacaa aaguaagacu cuggcaccaa caaggagaaa aaugccuaua uacggggaaa     1740

aacaucccaa ucucagaucu aauucauaac caauacaaau acgaaauaga ucacauacuc     1800

ccccuaaguc uuagcuuuga cgacagucua ucaaacaaag uuuuaguccu agcuacagcu     1860

aaucaagaaa aaggacgccg aacacccuuu caagcacuug auuccaugga ugacgcuugg     1920

ucuuaucggg aauuuaaaag uuaugugaaa gacagcaaau cguuaggaaa caaaaagaaa     1980

gauuaccugu uaaccgaaga agacaucuca aaaauagaag uaaaacaaaa auuuauagaa     2040

cggaauuuag uagacacccg cuacucauca agaguaguac uaaacacacu ucaagacuuc     2100

uacaaagcac acaaauucga caccacaauc agcguuguuc guggacaauu caccucacaa     2160

cucagacgua aauggggcuu agaaaaaucg cgggaaacuu accaucacca cgcggucgau     2220

gcucuaauca ucgccgcaag cucccagcua agacuaugga agaaacaaaa uaaucccuua     2280

auaucauaca aagaaggaca auuuguagac ucagaaacag gagagauaau aaguuuaacg     2340

gaugacgaau auaaagagcu agucuucaaa gcaccauacg aucauuuugu ggacacacuc     2400

agaucgaaga aauucgaaga uucaauucua uuuagcuauc aaguggauag uaaauuuaac     2460

agaaaaauaa gugaugcaac cauauacgca acuagaaaag cgaaacuaga uaaagacaaa     2520

uccgaagaaa cauacguacu cggaaaaaua aaagauauau acucccaguc cgguuacgau     2580

gccuuuauua aaauauauaa uaaagauaaa uccaaauuuc ugauguauca uaaagaccca     2640

caaaccuucg aaaaaguuau ugaagaaaua cuacgaaccu acccaucaaa agaguucaau     2700

gagaaaaaua aagaagucag uugcaacccc uucgaaaaau auaaacaaga aaacggacca     2760

auacgaaaau auagcaagaa aggaaacggc cccgaaauaa aaagucuaaa auacuacgac     2820

aaaaauauac cagaaucauu uauuaauauc accccaucca acuccaaaaa caaaguaauc     2880

uuaagacaau uaaacccuug gcgaaccgau guauauuuca accaucaaac agaaaaauac     2940

gaauuacuug gguuaaaaua ugcagaucuc caauucgaaa agaaaacagg aaccuacucg     3000

auuacuauag acaaauauaa cgaaauaaaa gaaaaagaag gaguaucaga acacucagag     3060

uucaaauuca cacuauauaa aaacgauuua cuuuuaauua aagauacaga aauaaaagaa     3120

gaacaaauau ucagauuccu aucaagaacc augccuaacc aaaaacacua cguagaacua     3180

aaaccauauu acaaaggaaa uuuugaaggc aaagaaagaa ucaaaguauu caauacaacc     3240

ucaaauggac gaugcuuaaa aggacuacga aagaaaggac uaaguauaua caaaauuaaa     3300

acagacguuc ucgguaauaa acauuuuaua aagaaagaag gcgaaaaacc caaacuaaaa     3360

uuuuga                                                                3366


<210>  5114
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5114
augaacggga aaauacuagg auuagauauc gguguggcau caguaggugu uggaauauua       60

gauaagaaaa cgggugaaau uauacacgca ucaucgagaa uauuucccgc cgcuacagca      120

gauuccaaug uagaacgaag aggauucaga caaggacguc gauugggcag aagaaagaaa      180

caccgcaaag uuaggcucgc agacuuauuc ucagacacag gacuaauaac agauuucuca      240

aaagugagua uaaauuuaaa uccuuaugaa uuacguauaa aaggccucaa ugaaaaacua      300

acaaaugaag aacucuuuau agcccugaaa aauauaguga aaaggagggg aauuucguac      360

cuagaugacg cuaacgaaga cggggaauca agcucaucag aauauggaaa agcugucgaa      420

gaaaaccgaa aacuacuagc ugacaaaacu ccaggucaaa uacaacuaga acgauucgaa      480

aaauauggac aaguuagggg agauuuuaca auagaagaaa acggagagaa acacagacua      540

uuaaacgucu ucucaaccuc ugcauauaag aaagaagcag aaagaauauu aacuaaacaa      600

caagauuaca aucaagacau uacagacgaa uuuauacaag cauacuuaac gauauuaacu      660

ggaaaaagga aguauuacca cggaccgggc aacgaaaaau cccgaacuga uuacgguaga      720

uucagaacag auggcacuac ucuagacaac auauuuggga uacuaaucgg aaaaugcaca      780

uuuuacccag aagaguacag agcggcgaag gcuaguuaua cggcacaaga guucaaucug      840

cuaaacgacu uaaauaaucu aacaguaccu acagaaacaa agaaauuaag cgaagaacag      900

aaaagacaaa uaauugaaua ugcuaaagga gcaaaaacau uaggcgcagc aacgcuacua      960

aaauauauag caaaauuagu agacggaagc guagaagaua uaaaaggcua ucgaauagau     1020

aaaagugaaa aaccagaaau gcacacauuu gacauuuaca ggaagaugca aacucuagag     1080

acaguggacg uagaaaaacu aucaagagaa guacuugacg aacuagcgca uauauuaacc     1140

cuaaauacag aaagagaggg aauagaagaa gcaaucaaag uaucauucau aaaaagagag     1200

uuugaacaag accaaauagc ugaauuggua ucauuccgaa aauccaacuc auccuuguuu     1260

ggaaaaggau ggcacaacuu cagcauaaaa cuaaugaccg aacuaauccc cgagcuauau     1320

gaaacaucgg aagaacaaau gaccauauua acaagacuag gcaaacagaa aacuaaagca     1380

agguccaaac guacuaaaua uaucgaugaa aaagagcuaa cagaugaaau auacaaccca     1440

guuguagcaa aaaguguacg acaagccaua aaaauaauua accuagccac gaagaaauac     1500

ggcguauucg auaacaucgu uaucgaaaug gccagagaaa acaacgaaga agaugcaaag     1560

aaagacuaug uaaaacgaca aaaagcuaac gaagaugaga aaaacgcggc uauggaaaaa     1620

gcagcgcacc aauacaacgg aaagaaagaa uugccagaca augucuuuca cggacauaaa     1680

gaacuggcaa ccaaaauaag auuauggcac caacaaggcg aaaaaugucu cuacacaggu     1740

aaaaacauac caauauccga uuuaauacau aaucaauaua aauacgaaau agaucauaua     1800

uuaccucuau cacucagcuu ugaugauuca cuagcaaaua aaguucuagu auuagccacc     1860

gcuaaccaag aaaaaggaca acgcacaccc uuccaagcgc uagacucaau ggacgacgca     1920

uggucauaca gagaguucaa ggcuuacguu cgcggagcac gcgcccuaag uaacaaaaag     1980

aaagauuauc uauuaaauga agaagauaua aacaaaauag aaguaaaaca aaaauucaua     2040

gaacgcaacu uaguugacac acgcuacucg agcaggguag uacuuaaugc auuacaagac     2100

uucuacaaac uaaaugacuu ugauacaaaa auaucugugg uccgcggaca auuuaccuca     2160

caacuaagaa gaaaauggcg aaucgacaaa ucaagagaaa cuuaucacca ucacgcaguu     2220

gacgcauuaa ucauagcugc aucuucacaa cuaagauuau ggaagaaaca aggcaaccca     2280

cucauauccu acaaagaaaa ucaauuuguc gauagcgaaa caggagaaau aauaucacuu     2340

acagacgacg aauauaaaga acucguauuc cgugcaccgu acgaucacuu ugucgauaca     2400

guaucaucca agaaauuuga agacagaaua cuauucuccu aucaagucga cagcaaauau     2460

aacagaaaaa ucagugaugc aacaauauac ucaacuagaa aagcgaaacu aggaaaagau     2520

aaaucagaag aaacauacgu acuaggaaaa auuaaagaua uauauacaca aacugguuau     2580

gacgccuuca uuaaacuaua caagaaagau aaauccaaau uucuaaugua ccauaaagau     2640

ccaauaacau uugaaaaagu aauugaagaa aucuuaaaaa cuuacccaga caaagaaauu     2700

aacgaaaaag gcaaagaagu agcuuguaac ccauuugaaa aauauagaca agaaaacggu     2760

ccgcuacgua aauacucgaa gaaaggaaaa ggucccgaaa uaaaaucccu aaaauacuac     2820

gacaacaaau uagguaauca uauagacauc acaccagaca acagcgaaaa ccaaguuaua     2880

cugcaaucac uuaaaccuug gagaacagac guauacuuua accauaaaac aaaaauauac     2940

gaauuaaugg gccucaaaua cucugaccua ucauuugaaa aaggcucagg aaaauauaga     3000

auaucacuag auaaauauaa cguaauuaaa aagaaagaag gcguacacaa agaaagugag     3060

uuuaaauuca cacucuauaa aaaugauuug auacuaauua aagacuuaga gaaaagcgaa     3120

caacaacucu uucgauauaa cagucgaaau gacacaucaa aacacuaugu agaauuaaaa     3180

ccauaugaca aagcaaaauu ugaaggaaac caaccauuaa uggcgcuauu uggaaauguc     3240

gcaaaaggag gacaaugucu aaaaggucuc aacaaagcua auauaaguau auacaaagua     3300

caaacagaug ucuuaggcaa caagagauuu auuaagaaag aaggugacgc accuaaacug     3360

gaguucuga                                                             3369


<210>  5115
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5115
augagugacu uaguacuugg auuagacaua ggaauuggca guguaggggu aggaauauua       60

aauaaaguua caggagaaau aauccauaaa aacagucgaa ucuucccugc agcacaagca      120

gaaaacaacc uaguaagaag aacaaacaga caaggaagaa gauuaacgag gaggaagaaa      180

cauagaaucg uaagacucaa uaggcuauuu gaagaaucug gauuaauaac ugauuuuaca      240

aaaauaucaa uuaaucuuaa cccauaucaa uuacgaguca aaggauuaac cgacgaacug      300

ucaaaugaag aacuauucau cgcccugaaa aacaugguca aacacagagg cauaucauac      360

cuagacgaug ccagugacga cgguaauucg agcgugggcg acuaugcuca aauagucaaa      420

gaaaacucaa aacaacuaga aacaaaaaca ccaggacaaa uacaacucga aagauaucaa      480

accuacggac aacuaagagg agauuuuacc gucgaaaaag acggaaagaa acacagacua      540

auaaauguuu ucccaaccuc agcauacaga ucagaggcuu uaagaauauu acaaacucaa      600

caggaguuca acucacaaau uacagaugag uucauaaacc gauaucuaga aauauuaacc      660

ggaaaaagga aauacuacca ugguccaggg aaugagaaaa gccgaacaga cuacggacgc      720

uauaggacga acggagaaac acuugacaau auauucggaa uacuuauagg aaaaugcaca      780

uuuuaucccg augaguucag agcugcuaaa gcaucauaca cagcacaaga auuuaacuua      840

uuaaacgacc ucaacaaucu gacuguaccg acggaaacua agaaacucuc aaaagaacag      900

aaaaaucaaa uaauaaauua ugucaaaaau gaaaaaguaa ugggaccugc aaaauuauuc      960

aaauauaucg caaaacuauu aucaugugau guagcagaca uaaaaggaca cagaaucgac     1020

aaaaguggua aagccgaaau ucacacauuc gaagcguaca gaaaaaugaa aacacuagaa     1080

acauuagaua ucgaacaaau ggauagagaa acuuuggaua aacuagcaua cguuuuaacu     1140

cugaacaccg aaagagaagg aauacaagaa gcacuggaac acgaguucgc agacggcagc     1200

uucucacaaa aacaaguaga cgaauuagug caauucagga aagcaaauuc aucaauauuu     1260

ggaaaaggau ggcauaauuu caguguaaaa cuuaugaugg aauuaauucc agaacuauac     1320

gaaacaucgg aagaacaaau gacaauauua acaagguuag gaaaacagaa aacaacgagc     1380

ucaagcaaua aaaccaaaua uauagaugaa aaauuauuaa cagaagaaau auacaauccc     1440

guaguagcca aaagcguacg acaagcuaua aaaauuguga augcagcaau aaaagaauac     1500

ggggacuucg auaacaucgu uauagaaaug gcccgagaaa caaacgaaga ugaugaaaag     1560

aaagcuauac agaaaauaca aaaagcuaau aaagaugaaa aagaugccgc aaugcuaaaa     1620

gcagccaacc aauauaaugg uaaagcugaa cuuccacauu caguuuuuca uggacacaaa     1680

caacucgcaa caaaaauacg auuauggcau caacaagggg aaagauguuu auacacagga     1740

aaaacuauuu ccauacacga ccuaauaaau aacccuaacc aauucgaagu cgaccauaua     1800

cuaccccuau caauaacuuu cgaugauagu cuugcaaaca aaguacucgu guacgcuacc     1860

gcuaaccaag aaaaaggcca aagaacacca uaccaagccc uagacagcau ggacgaugcu     1920

uggucauuca gagaacucaa agcauuugua cgagaaucua aaacgcuauc aaacaaaaag     1980

aaagaauacc uuuuaacuga agaagacaua ucaaaauuug acguaagaaa gaaauuuauu     2040

gaacggaacc uaguagacac acguuaugcu ucuagaguag uauugaaugc cuuacaagaa     2100

cauuucaggg cacauaaaau agauacaaaa guuucaguag uacguggaca auuuacaagu     2160

caacuccgaa gacauugggg cauagagaaa acaagagaua cauaccauca ucaugccgua     2220

gaugcauuaa uaaucgcagc aucgagccaa uuaaaucucu ggaagaaaca gaaaaacaca     2280

cuugucuccu acagugaaga acagcuccuu gauauagaaa cgggggaacu cauaagcgac     2340

gacgaauaua aagaaagugu auucaaagca ccauaccaac auuucgucga uacuuuaaaa     2400

ucaaaagaau uugaagauag cauacuauuu agcuaccaag uagauagcaa auucaacaga     2460

aaaauuuccg acgcaaccau auacgcaaca cggcaggcaa aaguaggaaa agacaagaaa     2520

gaugaaacuu auguucuugg aaaaauaaaa gacaucuaua cacaagacgg cuaugaugca     2580

uuuaugaaaa uauauaagaa agauaaauca aaauuuuuaa uguauagaca ugauccucaa     2640

acuuuugaaa aaguaauaga accaauacuu gagaacuacc caaacaaaga aaugaaugaa     2700

aaaggaaaag aaguucccug caauccguuc cucaaauaua aggaggagca cggauacaua     2760

agaaaauauu caaagaaagg aaacggaccu gaaauaaaaa gucuaaaaua cuacgacagc     2820

aaauuacuag gaaacccaau agauauaacu ccagagaacu ccaaaaacaa aguaguacua     2880

caaagucuaa agccauggcg aacagauguc uacuucaaua aaaacacagg aaaauacgag     2940

aucuuaggau uaaaguacgc agauuuacaa uuugagaaga aaacagggac auacaaaaua     3000

ucacaagaaa aguauaacgg aaucaugaag gaagaaggug uagacaguga uucagaguuc     3060

aaguucacac uauauaaaaa cgaccuuuua cugguaaaag acacagaaac gaaagaacaa     3120

caacuauuua gauuuuuauc aagaacaaug cccaauguca aauacuacgu ggaacuaaaa     3180

ccauauucua aagacaaguu cgagaaaaau gaauccuuaa uagagauauu gggaucugca     3240

gacaagucgg gaagaugcau caaagggcua gguaaaagca auauaucaau auauaaagua     3300

agaacagacg uauuggguaa ccaacacaua aucaaaaaug agggagacaa accaaaacua     3360

gacuuuuga                                                             3369


<210>  5116
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5116
augagcaaug gaaaaauauu aggcuuagac auagguauag caaguguagg cgucggagua       60

auagacgcac aaacaggaga aaucauacau gcaagcucaa gaauuuuucc aucggcaaau      120

gcagcgaaua acgccgagag aaggacauuu agaggaucac gaagacuaau ucgacgaaag      180

aaacaucgaa uaaaaagauu agaugauuua uucaaugauu uccacauuaa cuuagaugga      240

gaaaugagua cagacaaccc auauguacua agaguaaaag guuuaaguca aaaacuuaca      300

guagaagaac uauacaucag uaucaaaaac auaaugaaaa gacguggcau aaguuauuua      360

gacgaugcag aaucagauaa cgaagccgga agaagcgauu acgcaaaagc aauagaaaga      420

aauagacaac ugcuuacuuc aaaaacuccu ggagaaaucc aacuugaaag acuugaaaaa      480

uauggccaau uaagaggaaa cuuuacuaua auugacgaag aaggucaaag ccaacaaauu      540

auaaauguau uuagcacauc cgauuauguc aaagaaguag agaaaauccu agauugucag      600

aaaauguauc acaaauucau aucagacgag uucugugaca aauugauaga acuacucaga      660

gaaaaacgua aguacuacgu cggcccaggc aacgaaaaau caagaacaga cuauggaauu      720

uacagaacag auggcacgac auuagaaaau cucuuuggua uacuuauagg caaaugcaca      780

uuuuacccag accaauacag aucaagcaga gccagcuaca cagcacaaga auuuaacuuu      840

uuaaaugacc uaaauaaucu aacuguacca accgaaacua agaaauuaag ucaagaacaa      900

aaagaguucu uagucaauua ugccaaagaa acaucgguac uaggggcagg aaaaauacug      960

caacaaauag cuaaacuagc cgauugcaaa guagaagaca uacgcggaua ucgauuagac     1020

aacaaagaca aaccugaacu acauauuuuu gaaacuuaca gggcaaugaa agguuuaguu     1080

ccauuaguag acaucggagu gcuuucaaga gaacagcuag acauuuuagc agauauacua     1140

acuuuaaaca cagauuucga agguauaaga gaagcauuaa agaaacaacu ccccaacguu     1200

uuugaugaaa aacaaguaaa aggccucgcu agcuuucgca aaaguaaauc gcaacuauuu     1260

gcuaaagggu ggcauaaccu aagccagaaa auuauguuag aaguaauccc agaauuauac     1320

gccacaucag augaacaaau gacaauucua acaagguugg guaaauucga aaagaguucu     1380

guggcagaau auccaagcag uauaaaugua gacgaaauaa cagacgaaau auacaauccu     1440

guagucgcua aaucgauaag acaaaccaua aaaaucauaa acgcaucaau aaagaaaugg     1500

ggagaauuug accaaauagu uauagaaaug ccacgagaua gaaacgaaga ugaagaaaag     1560

aaaagaaucg cagacggaca aaaagccaac gcgaaagaaa aagcagacuc aauauuaaga     1620

gcagcugaac uuuacugcgc cgguaaagua cuuccugauu acgucuacaa uggacauaau     1680

caacucgcca ccaaaauaag acuuugguau caacaaggcg aaagaugcau auacacagga     1740

caaccuauaa gcauucauga ccuaauacau aaucagaacc aauacgaaau agaucacaua     1800

uuaccauuaa gccuaacauu ugaugauuca cuaaguaaca aaguacuugu ccuagcaaca     1860

gcaaaucaag aaaaagcaca gcguacucca uauaauuacu uaaaaucagc aacaucugca     1920

uggaguuaca gagaguucaa agauuacgua acaaaaagga aagggauagg uaaaaagaaa     1980

ugugaauauu uaacuuuuga agaagauauu aauggcuuug aaguaagaag uaaauuuauu     2040

cagcgaaauc ucguugacac cagauacgca agcaaaguua uauugaacgc guuacaagau     2100

uacuucaaaa uaucaggaau acaaacaaaa guaucaguug ucagagggca guucacaagc     2160

caacucagac acaaaugggg aauagagaaa acaagagaaa cuuaucacca ucacgcagua     2220

gacgccuuaa ucauugcugc aaguucccaa cuaagacuuu ggaagaaaca ggaaagccca     2280

cucgugguag acuaucaaga aggacgucaa guggaccuag aaaccgguga aauccuagaa     2340

cucacagaug aacaauacaa agagcuaguu uaucaaccac cuuaucaagg auuugugaac     2400

acaauaagua guucagcauu ugacaaugaa auucuauuua gcuaccaagu ugacaguaaa     2460

gucaauagaa aaauaagcga cgcuacuauu uacgcaacua gaaacgcaca auuaggaaaa     2520

gacaaaacag aagguauuua cguucucgga aaaaucaaag acaucuacac acaagcuggc     2580

uacgaagcgu uucucaaaag auauacaaaa gauaaaacau cauuccuaau guaucacaaa     2640

gaucucgaua caugggaaaa agucauugaa auaauucuaa gagacuacag agaauaugau     2700

gaaaaaggaa aagaaauagg gaacccauuc gaaagguaca gacgagaaaa cggcuacguu     2760

aagaaauauu cgcgcaaagg caaugggacg gcuauaaaau cauuaaaaua uuacgauaac     2820

aaauuaggga accacauaga cauaacacca gaaaacucua gaaacgcagu aguauuacaa     2880

ucuuuaaaac cauggcgaac agacguauau uucaacaaag aaacagguaa auaugaauuu     2940

uuaggcauca aauacagcga ccucagcuuu gaaaaaggaa cuggggaaua uggaauauca     3000

caagaaaaau acgauucaau aaaaaucgca gaaggagucg caaagaaauc aauuuuuaaa     3060

uuuaccuuau auaaacaaga ucuucuauuc auuaaagaca uagagaacaa uuucggaaaa     3120

cuauuaagau ucacauccaa aaaugacaca ucaaaacauu acguagaacu aaaaccauau     3180

gauaaaaaua aauuuggaac agaagaacca cuauuaccag uacuuggaaa cguagcaaaa     3240

agcggacaau gcauaaaagg auuaaacaaa ucaaacauau ccauuuacaa aguacgaaca     3300

gauauccucg gguacagaca uuuuauaaaa caagaaggag aacauccgca acuaaaauuu     3360

aaaaaguga                                                             3369


<210>  5117
<211>  3369
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5117
augaguaacg guaaaauauu aggccuagau auugguauag caucaguagg cguaggcguu       60

auagaugcac aaacaggaga aauuauucac gcaucuagua gaauauuccc uagugccaac      120

gcagcaaaca acgcagaaag acgaacauuc agaggcucga ggcgccuaau cagaagaaag      180

aaacaucgua uuaaaagguu agacgaccuu uucaaugacu uccauauaaa cuuagacggc      240

gaaaugucca cagauaaccc auacguuuua cgcgugaagg gauuaucaca aaaacuaacu      300

guugaggaac uauacauuag cauuaagaac auaaugaaaa gaagaggcau aagcuaucuu      360

gacgacgcag aaucagauaa cgaagcagga agaucggacu acgcaaaagc uauagaacga      420

aauaggcagc ugcucaccuc aaaaacgccc ggagaaauac aauuagaacg acuugaaaaa      480

uacggacaac uucgaggaaa cuucacgaua auugacgagg aaggacaaag ccaacaaauc      540

aucaauguau uuucaacauc cgacuauguc aaagaaguag aaaagauucu agauugucag      600

aaaauguacc auaaauuuau uagugaugag uucugugaua aacuaauuga acuacuccgc      660

gaaaagagaa aauacuacgu ugggccagga aacgaaaaau caagaaccga uuaugguaua      720

uauagaacag acggaacgac cuuagaaaau cuauucggua uacuuauagg gaaaugcaca      780

uucuacccag accaauauag aaguaguagg gccaguuaca cggcacaaga auuuaauuuc      840

cuuaacgacc uaaacaaccu uacaguacca acggaaacua agaaauuauc ccaagaacaa      900

aaagaauuuu uaguaaauua cgcaaaagaa acgucuguuc uaggagcggg aaaaauacuc      960

caacaaaucg caaaauuagc agauugcaaa guggaagaua uccgaggaua cagacucgau     1020

aauaaagaca aaccagaacu acacacauuc gaaacauauc gcgccaugaa aggucuaguu     1080

ccgcuuguag acauuggagu acuaaguaga gaacaacuag auauacuagc agacauauua     1140

acucuaaaca cagauuucga aggaauacgu gaagcacuaa agaaacaauu gcccaacgua     1200

uucgacgaaa aacaaguaaa aggacuagca ucuuucagaa aaagcaaauc acaacuauuc     1260

gcaaaaggau ggcacaacuu aagucagaaa auuauguugg aagucauacc agaacucuac     1320

gcaacaagug augaacaaau gacaauauua acuagauuag gaaaauucga gaaaagcuca     1380

guugcagaau accccaguuc aauaaacguc gacgaaauaa ccgacgaaau uuacaauccu     1440

guaguagcca agucuauacg acaaaccauc aaaaucauaa augcuagcau aaagaaaugg     1500

ggcgaauuug aucaaauugu cauagaaaug ccacgugauc gaaacgaaga cgaagaaaag     1560

aaacgaauag ccgauggaca aaaagcaaac gcaaaagaaa aagcagauuc aauacucaga     1620

gcugccgaac uauacugcgc cggaaaaguu cuaccagacu acgucuacaa ugggcauaac     1680

caauuagcua caaaaauacg acugugguac caacaaggug aaagauguau auacacagga     1740

caaccuauaa gcauacacga ucuuauacau aaccaaaacc aauaugaaau cgaccauaua     1800

cuaccucuau ccuuaacuuu cgacgacagu cuaucaaaua aaguauuagu acuagcaaca     1860

gcaaaucaag aaaaagcaca acgaacacca uacaauuacc ucaaaucagc gacaagcgcu     1920

uggucauauc gcgaauuuaa agauuacgua acaaaacgaa aaggaauagg uaaaaagaaa     1980

ugugaauacu uaacuuucga agaagauauu aacggauuug aaguacgauc gaaauucaua     2040

caaagaaacu uaguagacac cagauaugca ucaaaaguaa ucuuaaaugc acuccaagau     2100

uacuuuaaaa uaagcggcau ucaaacaaaa guuucaguag uacguggaca auucacuagc     2160

caauuaaggc acaaaugggg aauagaaaag accagagaaa cauaccauca ccacgcaguc     2220

gacgcucuaa uuauagcagc aucgucacaa cuacgguuau ggaagaaaca agaaagcccu     2280

cuaguagucg acuaucaaga gggaagacaa guugacuuag aaacuggaga gauauuagaa     2340

cuaaccgacg aacaauauaa agaacuugua uaucaaccgc ccuaucaagg auuugugaau     2400

acaauaucau cuuccgccuu cgacaaugaa auacucuuca gcuaccaagu agauagcaaa     2460

guaaacagaa aaauuucgga cgcaacaauu uacgcaacaa gaaaugcaca acucggcaaa     2520

gauaaaacag aaggaaucua cguuuuagga aaaaucaaag acauuuauac acaagccggu     2580

uaugaggcau uccucaagag auacacaaaa gauaagacau ccuuucuuau guaccacaaa     2640

gaccucgaua cgugggaaaa aguaauagaa auaauacuac gagauuaccg agaauacgau     2700

gaaaaaggaa aagaaauagg aaaucccuuc gaacgauaca gaagagaaaa uggauaugua     2760

aagaaauacu cuagaaaagg aaacggaaca gcaauaaaaa gccucaaaua uuaugauaac     2820

aaacuaggca accacaucga cauaacacca gaaaauaguc guaacgcagu cguacuacaa     2880

ucucucaaac cauggagaac agauguauac uucaauaaag aaacaggaaa auacgaguuc     2940

cucggcauua aauauagcga ucuuuccuuu gaaaagggaa caggagaaua cggcauauca     3000

caagaaaaau acgauucaau aaaaauagcc gaaggugugg cuaagaaaag caucuucaaa     3060

uucacccuuu auaaacaaga ccuucuauuc aucaaagaca uagaaaacaa cuucggcaaa     3120

uuauuaagau ucacaagcaa aaaugacaca aguaaacauu acguagagcu caaaccauau     3180

gauaaaaaua aauucgggac ugaggaaccc uuacugccag uguuagguaa cgucgccaaa     3240

aguggacaau guauaaaagg auuaaauaaa agcaacauau cuaucuauaa aguuagaacu     3300

gacauacuag gauaccgcca cuuuauaaaa caagaaggug aacacccaca auugaaauuc     3360

aagaaauga                                                             3369


<210>  5118
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5118
auggcaaaaa uauuaggacu agaccuagga acaaacagca uaggaugggc auuaauagau       60

gauguacaaa auaaaauuuu aggaguugga ucuagaaucu ucccaauggg cguagaaaau      120

cuaggagacg gcgaugggga aaucaguaaa aacgcaagca gaacgggcac ucgaggagug      180

agaagacaau ucuuucgaag aagauuacga aagaaaguau uauuaaaagc acuaagcgaa      240

aauaacaugu gcccaaugau cacaucagac uucgacaacu ggaaaaagac aaaacaauuu      300

ccaagugaaa aauuagcauc augguucgcu uuaaauccau acgaauugcg uaauaaagca      360

cuaagcgaaa aacuuacacu agaagaaaua ggcagaauau ucuaccaccu aauucaacgc      420

cguggauucu ugagcaauuc aagaaaagga ggcacagaug acggagcuau auucaaagga      480

aauccaaaag aaggcaaaau cggcauaaca gauacacaag agucuauaca agacaaaaca      540

cuagguagcu aucuauuuga aauauaucca aaggaaaauc aaccauuuca aaauggauua      600

gaaagaauaa gaaaccguua cacaguuaga aaaauguaug uagacgaguu cgaaaugaua      660

ugggaaaaac aggcucaaua cuauuccaau cucaaugaag aucucaaaac acuauuuggg      720

ggaagaaaau uagauggaua uaaagaagau ggaauacucu uucaucaaag acccuuaaga      780

ucacaaaaac accuagucgg aaacugugua uucgaaccaa acaaaacgaa auguccgauc      840

ucugcuaucc cauuugaaca auuuagagua uggcaauggg ucaauacagu agaauauaau      900

ggaaagaaaa uaagccaagu cgaaaaagag aaaauaguag aauuuuuaua ugcaaaugac      960

aaaacagauu uuaagaaaau aagaaaagcc auaggcaaag aauccacaga guucaaauuc     1020

aacuauaaag acgaugauaa aauaguggga acauacacag uuagccaucu cagcaacaag     1080

aaauauuuug gaaagaaaug guuugaauuu agcgaaaaag aacaagaaga cauauggcau     1140

auacuguauu ucuuugauuc uaaaucuaac uuaaaagaau acgcaauuaa aaacuggaau     1200

uuuacagagg aacaagcuga aaguauagcg aaauuuaaug ucaaagacgg uuacagcagc     1260

uuaucaagga aagcaauaag uaacauucua ccguucuuaa aacucggauu cacauacgac     1320

gucgcaguag ucuugggagg cauuaaaaac guuuuuggac augaauggga aaaacuuuca     1380

gaugaaaaga ggaacuuuuu aaucgacaau guagaaggca uugucagaag uaaaacaaaa     1440

ggaggauuca uagaugucau aaaagacaua uuaagaaaug auuauaauau aagcgacaac     1500

caacuucgua aacuauauca ucacucagcu acaaucuaug uugcggaacu uuuagaaaaa     1560

cuacccguug ggaaagaagc agauaaagaa auccaaucga uacgaaaucc aaucguaaua     1620

acagcuuugu ucgaauuaag aaaacuuguc aaugaacuga ucgacgauuu ugguucaauc     1680

gaugaaauca aaguagaaau ggcaagggau cuaaaaauau caaaaucgca aaggaauaaa     1740

auccgaagag aacaaaagag gcuagaaaga gaaaacgaua gagugaaagc aaggcucuua     1800

gaagagggcc aacgaaucac acaugacaac auacuacuau acaaauuaug ggaagagugc     1860

aagaaaacau gcccuuacac aggaaacccg auaccuuuau auaaacucuu cacuggagag     1920

auccaaauag aacauauaca ccccugguca agaagucuaa augauucauu ugcaaacaaa     1980

acacuauguu augcagauga gaacaggaag aaaggggaca aaacacccua cgaauuuuac     2040

ggaaacgacg agagcaacug gucagcaauu aaagaaagag ccuuaaaauu auuuucagac     2100

accaaagaau auccaaacgc cuaccaaaaa uuuaaaagau ucguccaaca uaaauucgau     2160

gaugacuuca guucacgaca acuuaacgac acucgcuaca uauccaaaga agcgaaaaau     2220

uacuuaagca aaauauguaa gaaaguagca guaagccccg gacaggcaac aucaaacuua     2280

cgacaaaaau ggggcaugaa uaauaucuua aacgaugaaa acgagaaaac acgagaagau     2340

cacagacauc augccauaga cgcguuggua auggcuugca caaaaauaag uuacguucaa     2400

gaauugagua aauggaaucg auauaauagg aacgcagaac uaaagaaauu ccccuuacca     2460

ugggaaacau ucuggucaga cgcaaaucgu gcaguagaca aaauucucau aucacacaag     2520

aaaguaucca acgauauaac aggcaggaca cacaucagug agaaaaaugg aaagaaauau     2580

aaaaacguag gaguagcagc aagaggacag uuacacaaag aaacaguaua cggaaaacga     2640

acuuuuaaug gagaagaagc cuuccacgua cguaaaacca uugauucacu gacgacagaa     2700

aaacaacuag aaaaagucgu agaugaauca auaaaacuau ugauaaagaa aagaguacaa     2760

gaacuaggag guuuuguuaa aggggcaaua ccagccaaua cauucuuugu aguagacgaa     2820

aacggaauaa aacaaccaca aaucuucuug ccaaauaaaa auggagcccc aguaccagua     2880

uugaaaguga gaaugaaaga aaauauagga ggagcagaaa aauuaaaaga aaauguuaac     2940

caauggguaa auccaagaaa uaaucaccau guauuaauau auaaagacga aaaaggaaau     3000

cuaaaagaag aaguagugac auucuggacu guaguagaaa gaaaacgaac gggacaauca     3060

gccuaccaau uaccauuuga uggaaaagaa auuguaacaa cccuacauau aaaugacaug     3120

uuucuuuuag gacuaaacga agaagaaaua aacugggaaa acccagacua ugaaguucua     3180

aaagaacacc uuuaucgcgu ccaaaaauuu acgagcggag auuauuacuu cagaauugcu     3240

aaaacuucaa guauucaaaa cgccaucgaa aaacaacaaa uaaauucuuu uggguuuggc     3300

aaaaauggau ggaguaccca uaacccaauc aaaguuaaaa uauccguaag uggaaaaauc     3360

ucaaaaguau ga                                                         3372


<210>  5119
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5119
augaacggau uaguucuugg cuuagauauc ggaaucggua gugucggagu aggaauccua       60

aacaaagaua cuggugaaau aauucauaca aacucaagaa uauucccagc agcaaccgcc      120

gauaacaacg uagaaagaag aaacucccga caagcuaggc gacuaaaucg aagaaagaaa      180

cauaggggag caagauugca cgaccucuuc gaagauuaug auuuauuaau agauuuuuca      240

aaaguaucua uaaauuuaaa cccuuaccgc cuacgagugc agggacugga caauccccua      300

accacagaag aacuauuuau ugcacucaaa aacauaguaa aacgacgagg aauaucauac      360

cuagaugaug ccagcgaaaa uggaggugca guaaguucag acuaugggaa agcaguagaa      420

gaaaaucgaa agcuacuagc ugaacagaca ccaggacaaa uacaacugga aagauuugaa      480

aaauauggac agguuagagg cgacuuuaca gucgaagaaa aaggcgacaa acaccgccua      540

auuaacguau ucucaacauc agcauacaga aaagaagccg aacgaauacu aaggaaacaa      600

caagaauuua auaaucaaau cacagacaaa uucauagaag auuaccuaaa aauacucaca      660

gagaaaagaa aauauuacca cggaccaggu aacgaaaaau cuagaacaga cuacggaaga      720

uuccgcaccg acggaacaac acuugauaac auauucggaa ucuuaaucgg aaaaugcaca      780

uuuuacccaa acgaauauag ggcauccaaa gccucauaua ccgcacaaga guucaaucug      840

cuaaaugauc uaaauaaccu cacaguacca acagaaacca agaaacucag cgaagaacaa      900

aagaaaacca ucguagaaua ugcuaaauca gccaaaacau uaggagcuuc aacccuucuu      960

aaauauauug ccaaaaugau cgacgcauca guugaucaaa uacguggaua cagaguugau     1020

gucaacaaca aaccagaaau gcacacuuuc gaaguuuaca gaaaaaugca aucauuagaa     1080

acaauccccg uaggcgaauu uucuagagaa gugcucgacg aacuugcaca cauacugaca     1140

uuaaacaccg aaagagaagg aauugaagaa gcaauuaaca caaaacuaau ggacguauuc     1200

agucaagacc aaauacuaga gcuaguacaa uuccguaaaa auaacuccag uuuauucuca     1260

aaaggguggc acaacuuuuc acucaaacuc augauggaac uaaucccaga acuauaugaa     1320

accucugaag aacaaauggc aauccuaacg agacugggaa aacaaaaauu caaagaaacc     1380

ucaaaaagaa caaaauauau agaugaaaaa gaaguaacug aagaaauuua caacccagug     1440

gucgcaaaau caguacgaca ggcaauaaaa auuaucaaug aggcaaccaa gaaauauggu     1500

auauuugaca acauaguuau agaaauggca agagaaaaca acgaagaaga cgcuaagaaa     1560

gacuacauca aacgucaaaa agcgaaccau gaugagaaaa augcugcaau ggaaaaagca     1620

gcauuccagu auaauggaaa gaaagaacua cccgauagua uauuccacgg gcacaaagaa     1680

uuggcaacaa aaauaaggcu cuggcaccag caaggggaaa aauguuuaua cacugguaaa     1740

aacaucccca uauccgauuu aauacauaau caauacaaau acgaaauuga ucacauacuc     1800

ccacuaagcc uaucauucga ugauagucua agcaacaaag uacuuguauu agccaccgca     1860

aaccaagaaa aaggccaaag aacaccuuuc caagcucucg auucaaugga cgaugccugg     1920

agcuaucgag aguucaaauc uuaugucaaa gacuccaaau uacuaggaaa uaaaaagaaa     1980

gacuaccuuc ugacagaaga agauaucagc aaaauagagg uaaaacaaaa auucauagaa     2040

agaaaccuag ucgauacacg uuauucaucg cgcguaguac uuaacacucu ccaagacuuc     2100

uacaaagcac acaaauuuga cacaacuaua aguguugucc gcgggcaauu caccucacaa     2160

cucagaagaa aauggggacu ugaaaaaucc agagaaacau aucaccauca cgcagucgau     2220

gcacucauca uagccgcuuc uucacaauua agacucugga agaaacaagc aaacccacua     2280

auaucauaca aagaagauca auucguugac agcgaaaccg gugaaaucau uagcuuaaca     2340

gaugacaaau auaaagagcu aguuuucaaa gcaccauacg cccauuucgu agauaccuua     2400

uccucgaaga aauucgaaga uaguauacua uuuucauacc aaguugacuc aaaauauaac     2460

cguaaaauau cagacgcaac cauauacgca acucggaaag uuaaacuuga uaaagacaaa     2520

ucugaagaaa cauacgucuu aggcaaaaua aaagauauuu acucacaagc cggguacgau     2580

gcauucauaa aaauauauaa uaaagacaaa aguaaauucu uaauguauca caaagaccca     2640

caaacauuug aaaaaguuau agaggaaaua cuuaggacau auccaucaaa agaguucaau     2700

gagaaaaaua aagaagugcc auguaauccu uucgaaaaau accgacaaga aaacggccca     2760

auccgaaaau acucaaagaa agguaacgga cccgaaauua aaagccuuaa auacuaugau     2820

aaaaauauac cagaaucauu caucaauauu acuccaagua acagcaaaaa caaggucaua     2880

uuaagacaau uaaauccaug gcgaacugac guauacuuca aucaccaaac agaaaaauac     2940

gaacuacuag gucuaaaaua cgcagacuua caauuugaaa agaaaacggg cacauauuca     3000

auaacaaucg auaaauauaa cgaaauaaaa gaaaaggaag gggucggaga acaaucagag     3060

uucaaauuua cacuuuauaa aaacgaccug uuacuaauca aagauacgaa aaacaaugaa     3120

aaacaaauau uccgauuccu aucaagaaca augccuaaug uaaaacauua cguagaacug     3180

aaacccuacg auaaagccaa auuugacgga aaccaaccac ugauaacaau auucggcaau     3240

gucgcaaagg gcggacagug ucuaaaagga cuaaacaaau cuaacauaag cauauuuaaa     3300

guaaaaacag auguacuggg caacaaacac uucauaacaa aagaaggaga aaaaccaaaa     3360

uuaaaauucu ga                                                         3372


<210>  5120
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5120
augaauggac uaguacuagg gcuugacaua ggcauagccu cagucggagu ggguauucua       60

aauaaagaaa caggcgaaau uauacacgua aacucccgca uauuuccugc ugcaaccgca      120

gauucgaacg uagaacguag aggauuucga caagguagac gcuuaggaag aagaaaaaaa      180

cacagaucag cccgcuugaa cgauuuauuu gaagaauucg gguucauaac ugacuuuuca      240

gcugucccuu uaaaccuaaa cccuuacgca uuaagaguaa aaggacuaag cgaagaacua      300

accaacgaag aacucuucau agcauuaaaa aacaucauca aaagaagggg aauaagcuau      360

uuagaugacg cuagugaaga uggagaaaca gccucgaacg aauacggaaa agcaguagag      420

gaaaauagaa aauuauuggc ugacaagaca ccaggacaaa uccaauuaga acgauuugag      480

aaauacggac agguaagagg agacuucaca guagucgaaa acggugaaaa ccaccgacua      540

auaaacguau ucucaacauc agcuuauaag aaagaagcug aacguauacu acguagacaa      600

caagaauuca augucagaau cucagacgaa uucaucgaag cuuacuuaac aauucuaacu      660

ggaaaaagaa aauauuacca ugguccugga aacgaaaaau cacgaacaga cuacggccga      720

uuucgaacag augggacaac cuuagauaau auauuuggua uauuaauugg aaaauguacu      780

uucuacccag acgaauaccg cgcagcaaaa gcauccuaua cagcccaaga auucaaccug      840

cuaaaugauu ugaauaaucu uacuguacca acagaaacaa agaaauuaag accagagcaa      900

aaacgacaaa ucguugaaua cgcaagaaca gcaaaaaccu uaggaacacc aacucuauua      960

aaauacauag cgaaauuagu agacggauca auagacgaua uaaaaggaua uagaaucgac     1020

aaaagcgaua aaccagaaau gcacaccuuu gaugcauaua gaaaaaugcg cacucuagaa     1080

cucguagaug ucgauauauu aucgagagaa acuuuagaug aucuagcaua uaucuuaacc     1140

uuaaauacgg aaagugaagg aauucuggaa gcuuuaaaua gcaaaaugcc uggaaccuuu     1200

acaaaagagc aaaucgauga gcuaauucaa uuuagaaaaa agaacagcgc agucuuugga     1260

aaaggauggc acaauuucuc acuuaaauua augaaugaac uaauaccuga auuauacgaa     1320

acaagugaag agcagaugac aauucugaca cgcuuaggaa aacaaagauc cagagaaaua     1380

ucaaaaagaa caaaauacau agacgaaaaa gaacuaaccg acgaaauaua uaaccccgua     1440

guagcaaaau caguaagaca agcgauaaaa aucauaaacc uggccacuaa aaaauaugga     1500

auauucgaua acauaguuau cgaaauggcc cgagaaagca acgaagacga cgaaaaaaaa     1560

gcgauccaaa auguccaaaa agcaaaugaa gaugagaaaa aagccgcaau ggaaaaagca     1620

gcagaccuau auaacggcaa gaaagaacua ccugauagca uuuuccacgg acauaaagaa     1680

cuagcaacaa aaauuagacu cuggcaccaa caaggcgaaa gaugccuuua uacgggaaaa     1740

aacauaagca uucaugaccu aauccauaac ccccaccaau augaaauaga ucauauauua     1800

ccauuaucau uaagcuucga cgauggacua gccaacaaag uauuaguacu cgcaacagca     1860

aaucaagaaa aaggucaacg cacacccuuu caagcacugg acuccaugga ugaugcaugg     1920

aguuauauug aauuuaaaca auacgucaga aacucaaaau cacuaaguaa caaaaaaaaa     1980

gacuaucuac ucaccgagga agauauuuca aagaucgaag uaaaacaaaa auuuauugaa     2040

agaaaccuug uagacacaag auacucgagc cgcgucguuu uaaacacucu ucaggaauuu     2100

uacaaaacca acgacuuuga uaccaagaua ucuguaguaa gaggccaauu uacaucacaa     2160

cuacgacgua aauggaaaau agaaaaaucc agagauacau accaccacca ugcaguagac     2220

gcacucauca uagcagccuc cucacaacua agauuaugga aaaaacaaaa uaacccauua     2280

aucaguuacc gcgaaggaca acuaguagau ccggaaacag gagaaauccu uucccugaca     2340

gaugaugaau acaaagaacu aguucuaagg cccccauacg acuauuuugu agacacauua     2400

aaaucaaaau cuuucgaaga caguauauua uuuucauauc aaguagacag uaaauacaac     2460

cggaaaauau cugaugccac aauauacggc accagaaagg cacaacuagg gaaagacaaa     2520

caagaagaaa cauaugucuu aggcaaaauc aaagacauuu acucacaaaa aggcuacgag     2580

gacuuuauua aacgauauaa aaaagauaca acccaauuuc uuauguauca caaagaccca     2640

caaacguucg cgaaagugau agaggaaaua cuaaaaacau acccagacaa agaacucaac     2700

gaaaaaggua aggagauacc augcaaucca uucgaaaaau acagacaaga aaacgggcca     2760

auccgaaaau auaguaaaaa aggaaaaggg ccagaaauaa aaucacuaaa auauuacgac     2820

aauaaacugg ggaaccacau agauaucaca ccagugaauu cucaaaacca agugguacug     2880

caaucacuaa aacccuggag aaccgacguc uauuucaacc cucaaacauc aaaauacgaa     2940

uuaaugggac ugaaauacuc agaccuuaga uucgaaaaag guucagguag cuauggaaua     3000

agcccagaaa aauacaauaa agucaaagca aaagaaggag uggacgaaga uagugaauuc     3060

aaauucaccu uauauaagaa cgaccuaaua uuaauaaaag acacugagac gggagaacaa     3120

caauuauuca gauauggauc cagaaacgac acaucaaaac auuacgugga acuuaaaccc     3180

uacgaaaaag ccaaauuuga aggaaaccaa caauugauga aucuacuugg aacaguagcu     3240

aaagguggcc aauguuuaaa gggaauaaac aaaccuaacc uuucaauaua uaaagucaaa     3300

acugacgucc uaggaaacaa auauuucaua aaaaaagaag gagaccagcc ucaauuaaau     3360

uucaaaaaaa aaauauga                                                   3378


<210>  5121
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5121
augaccagca aacaagaaaa uuuaggagca uuaacguucg gacuugacau agggauugca       60

agugugggcu gggccgugcu gggcgaaaau agaauaauag acuuaggggu caggugcuuu      120

gacaaagccg aaacagcaaa agagggagaa agccugaauc ucgcaagacg aauggcuagg      180

uuauugagac guagauuaag aagacgcgca uggagauuaa caaaauuagc cagauuacua      240

aaaagagaag gauugauagc agacgcgaau cuguuuaaaa auaaaauaga gaaaaauacc      300

ccuguuccca aaaguaguug gcaauuaaga guagacgcau uagaccgaaa acuaaacgcc      360

gaagaauggg caagaguaau uuaucaucua ugcaaacaca gaggauucca cuggauauca      420

agagcggaag aaaaagcagc ugaauccgau uccaaaggag aaagcggaaa aguuaaacag      480

ggauuagccg gaacaaagaa acuaaugcaa gagaaaaauu acagaacagc agcagaaaug      540

guacuagcag aguucccaga agcacagagg aacaaacaag gagauuacuc aaaagcauua      600

ucaaggaucc uccuagguga ugaauuaaag acauuauuug aacaccaacg aaaacuagau      660

ucaccuuuug caacggaaaa auuagaagca gcgauauugg gaaauggcga cagaaaauca      720

ggcuuauuuu gggcgcaaaa cccagcguua gcaggaagug auuuguuaaa aauguuaggu      780

uauuguacuu uugagaaaac cgaauaucga gcaccaaaag ccagcuucac agcagaacgu      840

cacguauggu uaacaagacu aaauaaccua agaauaguag uagaugguac aacacgacca      900

cuaaacgaag cagaacgcag aauugcacua uuaaugccau aucaacaagc cggcgauuuc      960

acauacaaac aacuuaggac ugcguuaacu aaagcagguu uucuaccaga aucauuuagg     1020

uauacaggau uaucauacgc uaguggacaa aaagcaggcg agaaaacuaa agacccugaa     1080

agcgcugcuc ucguuaaauu cccugcaugg caagaauuac guaaaacacu agaaaagaaa     1140

gaacuagcuu cagaauggca aaagauauca acugaugcgc uagacggaaa accacagcua     1200

cuagaucaaa uagcuugggu gcucagcgua uauaaagaug augacgaagu aaaggccgaa     1260

uuaucgaaac uuaaucuacc aaacgccgag aaaaugauag acgcacuacu ggauauaaga     1320

uuugacaaau uuucuaauuu gagcuuaaaa gcauuaugua aaauuguacc uuuaauggaa     1380

caaggucaaa gauacgacga agcuauagcg aauauaccag aauacgguca ucacagucaa     1440

cuacauaaag uaggggcagg agaacauaaa uauuuaccac ccuucuacaa ggaacgagau     1500

aagacaggaa gaaugguauu uaaugacgac auggauauac ccaggaaucc aguaguuuua     1560

agagcauuaa aucaagcgcg uaaagucgua aaugcuuuaa ucaaaaagua ugauucaccu     1620

caugaagugc auauagaaau ggcucgagau uuaucaagac cacuugacga aagaagagaa     1680

gucgaaaaac uucaaaagga auuucgagac aacaacgaaa aggguaaagc acaauuugca     1740

caagacuuca auauaguagg cgcgguaaaa gggaaagaau uugagaaaua ccaacuauau     1800

cgagaacaac aagguaaaug cgccuacuca auagaaccua uggacauuca cagacuauuu     1860

gaacaaggau acgucgagau ugaccacgca cuaccauacu caagaaguuu ugaugacagu     1920

aaaaauaaca gagucuuagu ucuagcaaaa gaaaacagag auaaagggaa ucgaacacca     1980

uucgaguauc ucggaggaaa agacaauuca gaaccauggc gacaauuugu ugcuuuuguu     2040

gagggaaaua aagcauauag acuggcaaaa agaucaagau uacuaagaaa agacuucgga     2100

gauaaagaag caaaggaguu ccgcgaaaga aaccuaaacg acacaagaua cauuuguaaa     2160

uucuuuaaaa acuauguaga acaguaucuu cagcuacacg aagacucaga agcaaaacgu     2220

ugcguaguuc uauccggaca aaugacaucg uuucuaaggg cacguugggg auuaguaaaa     2280

guuaggggug acagcgaccg acaucaugca cucgacgcag cugucguugc ugcuugcuca     2340

cauggaaugg uuaaaagacu aucugacuau agcaggagga aagaacuuga acaggucaga     2400

gagggauuug uugauguaga caccggggag auagucaauc cagcaauguu ccaacaaguc     2460

caagagcauu uucccaaucc auggccucau uuuagagaug aagucaaauu aagauuauca     2520

auggaugacc cagcuuuguu acggagcgaa auggaaaagu ucggcacuua cacaccggaa     2580

gcacuacagg cacuacaccc uuuauuuguc uccagagcac cucaaagaag aaauucagga     2640

gcagcacaua aagauacuau auacgcucaa ccgaaaagau uaguagccca aggaggcgua     2700

acacaaaaag uugcacuuac aaguuugaca uuaaaggacc uagauaaacu aauagaucca     2760

caccguaaug auaaacuaua cacagccaua agagcacgau uagaacaaca cggagguaaa     2820

ggagacaaag cguucccaag cucuaacccc uuacggaaac cagauaguga gggaaaucca     2880

acuggaccaa uagugcgcgg cgucacaaug guaauagaua aacuaucagg aaucccuaua     2940

agagggggaa uagcuaaaaa ugacucuaug guaaggguag auguauuuac gaaagccaau     3000

aaguuccauu uaguuccagu cuauguccau cacacagugg cuaaagcccu accuaauagg     3060

gcaguaguag cuuucaaaga cgaagaugag uggacauuaa uugaugacag cuuugauuuu     3120

uguuuuucgu uacacccgaa cgaccuagua caaguacaac aaaaaggaaa agcaggaaug     3180

auuggauauu auagcagcug ccacagagga acaggaaaca uuaauuuaug ggcacaugac     3240

cgaaacuuac agguaggaaa agauggaaug auagaaggua uuggagugaa aacagcauug     3300

ucacuagaaa aauucaacgu cgaugugcua ggcaaccgau acccagcacc accggaaaaa     3360

cgaagagauu uagcauga                                                   3378


<210>  5122
<211>  3378
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5122
augacagaca gaaucucauu aggacucgac aucgggguag caagcguugg uuucagcgua       60

cuagauauag acaaaggaaa aguaauagaa cuaggagcac gauuauuuaa cgcaacaguc      120

gcggcaggaa aucaagacag aagagacaug aggggcucaa gacgacuauu gaacagaaac      180

aaacagagac gaaaagacgu aucuaaauua uuucaagaau acggacuguu ggacaacuuc      240

gacagagaca acuuucacac agccuuuaau aacaauugga auccauauga gcuacguguu      300

aaaggcuuaa caaaacaacu aaauaaagaa gaacucgcag acucauugua ucaaauaaua      360

aaaagacgag ggauaagcua ugcauuaaaa gacgcugacg caaacgaaga cggaacagau      420

uacucaucau ccuuaaaaau uaauucgcaa gaacucauag aaaaaacgcc agcgcaaauu      480

cagcuacaac gacucaacga uuacggcaaa guacgaggca aaguaaucgu cggagacgac      540

aucgacgacc aaaaaguacu uuuaaacgua uucccgacca augcauacga aaaggaggca      600

cgccaaauaa uagcuacaca acaacaauuu uaucccgaaa uauuaacagg agaauuugua      660

aaagaauacu gucagauauu aacacgaaaa cgagacuauu ucguugggcc aggcaacgaa      720

aaaucacgaa cagacuacgg aauauauaaa acagacggaa gaacauugga caaucuuuuu      780

gaagaauuaa ucggacauga caaaaucuac ccagacgaac uacgagcguc agcagcuuca      840

uacaccgcuc aacucuuuaa cgugcuaaac gaccuaaaua auuuaagaau auuaagcuac      900

gaagacggaa aauuaacaca aauagacaaa gaaaaaauca uagcagaaau aaaaaauaau      960

accacaguag ucaacauguu aaacguuaua aaaaaaguaa ccggcuguca aaaagacgau     1020

aucaaaggau ucagacuuaa ugaaaaagac aaaccagaaa uuucaucaau gccuauuuac     1080

agaaaagcgc auaaggauuu ccuaaaggua ggcaucgaua uaacugacug gccaauagac     1140

uucaucgaca gacuaagcuu uauauuaaca uuaaacaccg aaaauggaga gauaagaaaa     1200

caaauaaaca aaagauuagu ccccgacuuu gacuucuuaa augacgaucu aguacaacuc     1260

auuaucgaua acaaagacag cuucgacaua aaaacaaaua acaaauggca ucgauuuucc     1320

uuaaaaacca ugaacaaacu aauccccaca augaucgaaa gaccaguaga gcaaaugacu     1380

cuccucacag aaaugggccu aauaaaaaaa gauacuaaaa gauucgaagg aaauaaauau     1440

cucccauaca aagaaauagc uaacgauaua uuuaacccgg uagcgucaaa aucaguaaga     1500

gaagcucuaa aaauagugaa cgcaguuuua aaaaaauaug gacauauaga auacauagua     1560

auagaaaugc cgagggauaa aaaucuugac gaagaacgca aacaaauagc cgacuuccaa     1620

aagaaaaaua aaaaaauaaa agacgcagca uucaaugcgu ucguaaaagc agucgguuca     1680

aaaaaaaaug uaaaaauugc acuaucaaaa aacaggaaau uacaaauggg aauacgauua     1740

uggcaucaac aacaaggcau cgauccauac aauggggaag aaauaaacgc gaacgaucua     1800

auaagcaacc cagacaaauu cgaaauagau cacauaauac cacaauccau uagcuucgau     1860

gauggaauaa auaacaagac guuaugcuuc gccucgauga aucaaguaaa aggacaaaag     1920

acaccauacg aauuuuuaaa cgaaggccac ggacagggcu acgcaaaauu caaagcgaua     1980

gucuccaaga acaaaaacuu cucaaaagcu aaaagagaua acuacuuauu cgaagaaaac     2040

gucucaaaca ucgaaacaag aaaaagguuc uuaucgagaa accuagucga cacacgcuau     2100

aguagccgag uaguucuaaa cucacuacaa gaauuuuuuc acgaaaaaag ugaugacaca     2160

aaagugacag ugauaagagg aaaauucaca uccaacauga gaaaacauug gcacaucgac     2220

aaaacuagag auacauuuca ucaucaugca auagacgcaa gcauuauagc agcaacaccu     2280

uuccuccgca uguggaaaaa aggcagcaca auauucccua ccaaaaucaa cgaaacauca     2340

auagacauag aaaccggaga aauacucgac gauaaaaauu uugauaaagu aauguacgaa     2400

gaacccuauu ccggguucau aagcgaaaua augaacgccg augaccgaau aaaauucagc     2460

caucaaguag acaagaaaau gaauagaaaa guaucagacg caacaauaua uagcacacgu     2520

auaggaaaac uaaguaaaga uaaaaaagau gcggaauacg uaguagcaaa agucaaagac     2580

auuuauucgg uagauggaua ugagaacuuu aaaaaaguau acaaaaaaga uaaaacaaaa     2640

uucuuauuau acaaguacga uccacgaaca uuuucagaau uggaaaaaau cgucauagac     2700

uauccagaca aaaucgagaa aguccagacc aauggcaaaa uuaaagcagu agauaucagc     2760

ccuuucgaac uauacagaag agauaacgga auggugagaa aauauucuaa aaaaaccgga     2820

cccgccauaa aacaacucaa guaucuagac aaaaaacuag guucacacau agacauaacc     2880

ccaaaaaacg caaacaacaa acauguagug cuacaaagcc uuaaaccaug gagaacagac     2940

guuuauuuga accaugaaac uggggaauac gaaaucaugg gcauuaagua uucagaccug     3000

aaauuuaaca aaaacgaagg auacggaaua aaaaaagaca aauaucuuaa aauaaaaaau     3060

auagagggcg uaagugaaaa cucugaauuc auguuuagcc uauaccgaaa agaucggaua     3120

aaaguacaag auauggaaac aaaugaaucg guugaauuac uauucugguc aagaaauuuu     3180

agcaacaaga aguaugcaga acugaaaccc auaagccaaa uagauaauga uaaaguuuua     3240

cccauauaug gaagagguag acuuauaaaa agacuaaucc caaaaaauug uaagaucugg     3300

aaagucaaca caaguauauu aggugacaca uacuacuuag aaaaagaaag uaacuauccc     3360

caaaagauac uagauuga                                                   3378


<210>  5123
<211>  3381
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5123
augaacggcu uaguacucgg uuuagacauu ggaauugccu caguaggugu cggcauccuu       60

aauaaagaaa cgggcgaaau aauacacgcc aauaguagaa uuuuccccgc ugcgacagcc      120

gacucaaacg uggaaagaag gggauucaga caaggaagaa gauuaggcag aagaaagaaa      180

cacaggucag cgcgacuaaa uaacuuauuu gaagaguucg gauucauaac agacuuuucc      240

gcaauaccac ucaaucuaaa ccccuaugcg cuacgaguaa aaggacuauc agaagaacua      300

acaaaugagg aacuauucau agcccuaaag aacauuauaa aacgaagagg aauaucauac      360

cuagacgaug caagugaaga uggugaaacu gcaucgaaug aauaugguaa agcugucgaa      420

gaaaauagaa aacuauuagc agacaaaacu cccggacaaa uccaacuaga aagauucgaa      480

aaauacgguc aagugcgugg agauuuuacg guaguggaga acggagaaaa ccauagguua      540

auaaacgucu ucucaaccuc cgcauacaag aaagaagcag aaagaauauu aagaagacaa      600

caagaguuca acguccgaau cucggacgag uucaucgagg cuuaccuaac aauauuaaca      660

ggaaaaagga aauauuacca uggaccaggg aaugagaaaa gccgaacgga uuacggacga      720

uuccgaacag auggaaccac acucgacaau auauuuggca uuuuaauugg caaaugcaca      780

uucuacccag augaauauag agcagcaaaa gcaagcuaua cggcacaaga auuuaaccua      840

cuuaaugacc uaaauaacuu aacaguuccu acagaaacua agaaacucuc acccgaacaa      900

aaacgccaga uagucgaaua ugcaagaaca gcgaaaacac ucggcacucc uacacuacua      960

aaauacauag cgaaauuagu ggacgggucu auagaugaua uaaaaggcua cagaauagac     1020

aaaagugaca aaccagaaau gaugcauacg uucgaugccu aucgcaaaau gagaacucua     1080

gaucuuguua acauagaugc auuaucgaga gaaacacuug acgaccuagc acacauauua     1140

acccuaaaca cagaaucuga aggcauccua gaagcacuaa acaguaaaau gccaaguaca     1200

uuuacuaaag aacagaucga ugaauugaua caauucagaa agaaaaacag ugcagucuuc     1260

ggcaaaggau ggcacaacuu uagucuaaaa cugaugaacg aacuaauuuc cgaacuuuau     1320

gaaaccagcg aagaacaaau gacuauucua accagacuug gaaaacagag aucaagagaa     1380

auaucaaaaa gaaccaaaua uauagaugaa aaagaacuua ccgaagaaau auacaaccca     1440

guaguagcca aaagcguuag acaagcgaua aaaauaauaa acgaagcgac aaaacgcuau     1500

ggaaucuuug auaacauugu aauagaaaug gcacgagaaa acaacgaaga agacgccaag     1560

aaagacuaca uaaaacgaca aaaagccaau caagaugaga aaaacgcguc aauggaaaaa     1620

gcagcauuuc aauacaaugg uaagaaagaa cuaccagaca guauauuuca cgggcacaaa     1680

gaauuagcca caaaaauaag acuauggcac caacaaggcg aaagaugucu cuacacuggc     1740

aaaaacaucu cgauccgaga uuuaauacac aacccacauc aauacgaaau cgaucauaua     1800

uuaccauuaa guuuaucauu cgacgacgga cuagccaaua aaguauuagu acuagcuacg     1860

gcaaaccaag aaaaaggaca aagaacaccc uuccaagcua uagacucaau ggaugacgca     1920

uggucuuaua gagaguucaa acaauaugua agaaauagca aaucacuauc caauaaaaag     1980

aaagauuacc uacuuacaga agaagauauc aguaaaauug aaguaaaaca aaaauuuaua     2040

gaaagaaauu uaguagacac aagauacagu ucuagaguag uccuaaacac acuucaagaa     2100

uuuuacaaaa ccaacgacuu ugauacaaaa auuuccguag uccgcggaca auuuacauca     2160

caauuaagaa gaaaauggaa aauagaaaaa ucaagagaua cauaccacca ccacgcagua     2220

gacgcccuua uaauagcagc cagcagccaa cuucgauuau ggaagaaaca gaacaacccc     2280

cugauaucau acaaagaagg gcaauuugua gacccagaaa caggagaaau auuaagccuc     2340

acagacgacg aauacaaaga acucguauuu aggccacccu acgacuacuu ugucgacaca     2400

cuuaaaagca aaucguucga agacaguauu cuauuuaguu accaaguuga uagcaaauac     2460

aauagaaaaa uaagcgacgc cacuauauac ggaacccgaa aagcucaauu aggaaaagau     2520

aaacaagaag aaacguacgu acugggaaaa auaaaagaca uauacagcca aaaaggcuac     2580

gaagacuuua uaaaaagaua caauaaagac gaaacacagu uccuaauaua ucacaaagau     2640

ccacaaacau ucgaaaaagu uauagaagaa auacuaaaaa ccuauccaga caaagaacua     2700

aacgaaaaag gaaaagaaau accuugcaau ccguucgaaa aauaucgcca agaaaacgga     2760

ccaauacgaa aauacucaaa gaaaggaaaa gguccugaga uuaaaauacu aaaauauuau     2820

gauaacaaac uugguaacca cauagacaua accccaguca auucacaaaa ccagguaguc     2880

cuccaaagcc uaaaaccuug gcgaacagau guuuacuuca acccaagaac auccaaauac     2940

gaauuaaugg gccuaaaaua uagugaucuc agauucgaaa aaggaagcgg uucauacggu     3000

auaaguccag aaaaauacaa caaaguaaaa gcaaaagaag ggguuaacga agacucagag     3060

uucaaauuca ccuuauauaa aaaugaccuu auucuaauaa aagacacaga aaccggcgaa     3120

caacaauuau ucagguacgg cucaaggaac gauacuucga aacacuaugu agaauuaaaa     3180

ccuuacgaaa aagcaaaauu cgaaggaaau caacaacuaa ugaaucugcu cgggacaguu     3240

gcuaaaggag ggcaaugcuu aaagggaauc aauaagccaa aucuaucaau uuacaaagua     3300

aaaaccgacg uguuaggcaa uaaauacuuc auaaagaaag aaggagauca accacaacua     3360

aacuuuaaaa agaaauuuug a                                               3381


<210>  5124
<211>  3381
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5124
auggcgagua aacaugaaaa ucucggagca uuaacauuug gauuagacau cggcauagcg       60

aguguuggau gggcuguacu aggagaaaau cgaauaauag aucuaggagu acgauguuuu      120

gauaaagcug aaacugcgaa agaaggugaa ucauuaaacc uagcuagacg aacugcaaga      180

cuauuacgaa ggagacuaag aaggagagca uggcgucuaa caaaacuagc aagauuacua      240

aaacgagaag gauuaaucgc agacaccaau guuuuucaaa aaccauucaa ucuaaaagac      300

gcaaacgaaa aaggacuuag uuucuggcaa cuaagaguag augcucuaga cagaaaauua      360

uccgccgaag aaugggcaag aguuauauau cauuuaugua aacaccgagg uuuucauugg      420

auaagucgcg cagaagaaaa agccgccgag ggcgacagca aauccgaggg aggaaaagua      480

aaacagggac uugcagggac uaaaaagcua augcaagaga aaaauuauag aacagcagcu      540

gaaaugguau ugacagaguu cccagaagcu caaaggaaua aacaagguga cuauaguaaa      600

gcccugagca ggauacuuuu aggugaugaa augacucaac uauucaaaca ccaacgagaa      660

uuaggaaacc cacaugcgac cgaaaaauua gaagcugcaa uucuaggaaa cggagaccau      720

aagagcggau uguuuugggc acaaaaucca gcccuagcag gaaacgacuu acuuaaaaug      780

cuuggacauu guaccuucga aaaagcagaa uaucgaguac caaaagcauc auuuacagcu      840

gaaaggcaug uuuggcuuac uagauuaaac aauuugcgca uuguugucga uggaacaaca      900

agaccuuuaa augaagccga aagacauuua gcauuauuaa ugccuuacca acaggcggga      960

gaucuaacau acaaacaauu aaggacugca cuaacuaaag caggauaucu gcccgaaucg     1020

uuccgauaua cagguuuagc guacgcauca gggcaaaaag cagaugaaaa agcgaaagac     1080

ccagaaagcg caccucuagu aaaauuacca gcauggcaug aacuaaggaa gacauuagag     1140

aaaaaggaac uagcuaguga auggcagaaa auaagcucug augcauuaga cggaaaacca     1200

caacuauuag aucaaauagc auggguccuu agcguauaua aggaugauac agaagucgaa     1260

gcagaauuau gcaaacuaaa uuuaccuaau gccgagaaaa ugaucgacgc acugcucgac     1320

auaagguuug auaaauucag uaaccuaagc cuuaaggcuu uaagacaaau aguaccccua     1380

auggaacaag gucagagaua ugacgaagca guugcuaaua uaccugaaua uggacaucau     1440

ucucaacuac augaaguagg agcuggggaa cauaaguacc uaccaccuuu uuacaaagaa     1500

cgagauaaaa cagguagaau gguauuuaac gaugauaugg acauaccaag aaacccuguc     1560

guacuaagag cacuuaacca agcacgaaaa gucgugaaug cuuuaauaaa agaauacggc     1620

aguccccacg aaguacacau agaaauggca cgagaccuaa gucgcccauu agaugaaaga     1680

cgagacguag aaaaauuaca aaaagaauuu agagauagaa acgaaaaaga caaggcuuua     1740

uuugcacaag auuucaacau uguaggagcu guaaaaggaa aagaauuuga aaaauaccag     1800

uuauauagag aacaacaagg gaaaugugca uauucaauag aaccaaugga uauucacaga     1860

cuuuuugaac aaggauacgu ugaaauagac cacgcauuac cauauaguag aucauuugau     1920

gauagcaaaa auaacagagu ucuaguauua gcaaaagaaa auagagauaa ggguaauaga     1980

acacccuuug aguaucuugg uggaaaagac aacucagaac gauggagaca auuuguugca     2040

uucguagaag ggaauaaaag cuaucgauua gccaaaagaa guagacuguu acguaaggac     2100

uuuggaaaag acgaagccaa aggcuuuaga gaacgaaacc uaaaugauac uagguacauu     2160

uguagauucu uuaaaaacua cguagaacaa uaccuacaac uucacgaaga uagugaugcc     2220

aaaaggugug uaguacuaag cgggcaaaug acgucauucc uaagagcaag auggggucua     2280

guaaaaguca ggggagauag cgauagacau cacgcacuug auggggcgau aguugcagca     2340

uguucacaca guaugguaaa aagacuaagu gacuauucac ggcgaaaaga auuagagcaa     2400

guucgagcag gguucgucga cguagauaca ggagaaauag uuaauccagc aauguuucaa     2460

caaguacaag aacacuuccc gaaccccugg ccacauuuca gagaugaagu aaaauuacga     2520

cuaaacgucg acgauccagc auuauuacac ucugaaauga acagauucgg uacauauucu     2580

ccggaagccu uaaaagcucu acgcccauug uuuauaucac gggcaccgca aagaagaaau     2640

aguggugcag cacauaagga cacgauauau gcgcaaccag aacgauuagu agcacaaggu     2700

gguguaaccc agaaaguccc acuaaccucu uuaacacuaa aagauuuaga uaaacuuaua     2760

gacccacauc gaaaugaaaa auuauacucc gccauaagaa uacgucucga acaacaugga     2820

ggaaagggcg auaaggcuuu cccugcaucu aaucccuuaa gaaaaccuga uagcgaagga     2880

aacccuaccg gaccaauagu aaggggaguc acaaugguua uagacaaauu aucugggaua     2940

ccuauaagag gagguauagc uaaaaacgau ucgaugguac gaguagaugu auuuacuaaa     3000

gcugguaaau uucaccuagu ccccguuuac guacaucacg caguugcaaa aguacuccca     3060

aaucgagccg uaguggcaua uaaggaugaa acagaaugga cucuaaucga ugauggcuuu     3120

gauuucugcu uuaguaugua ucccaaugau cuccugagaa ucaaacuaag aaaugcuagu     3180

cacuuaggau acuaugcaag uuguaacagg ucaacgggag caauaaauuu guggagccau     3240

gaccgaaacc aacaagucgg aaaagacggg augauagaga gcauaggugu aaaaaccgca     3300

cugaaugugg aaaaacuaaa cauagacgua cuagguaaua gauacccagc gccaccugaa     3360

aaacgacgag acuuagcgug a                                               3381


<210>  5125
<211>  3384
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5125
augagcgauc ucguccuggg uuuagacauc gguaucggga gcguaggagu agguauacuu       60

aacaaaguaa caggcgagau aauucauaag aacucaagaa ucuuuccugc agcccaagca      120

gagaauaaug uagaaaggag aaucaaccgc caaggaagaa gauuaaccag acgaaagaaa      180

caucgcaggg uaagacuaaa ucaccuauuc gaagaauccg gacuaauaac cgauuuuacg      240

aacguaagua uaaauuuaaa uccauaucaa cuacggguaa aaggacugac agaugaacua      300

agcaaugaag aacuauucau agcacucaaa aauaugguaa aacacagagg uauuucauac      360

cuagaugacg caagugacga uggaaauagu uccguuggag auuaugcaca aauaguaaaa      420

gaaaauucaa aacaacuaga aacaaaaaca ccgggccaaa uacaacuaga aagauaucaa      480

aaauauggac aauuaagagg agacuucaca guugaagaag acggaaagaa acacagacua      540

auaaauguuu ucccaaccag ugccuacaga gccgaagccu uacggauacu ucaaacacaa      600

caagaauuua auccacagau aacugaugaa uuuauuaaua guuaccuuca aauccuaacu      660

ggaaaaagaa aauacuauca cggaccaggc aaugaaaaau cccgaacuga cuacggaagg      720

uauacgacaa agaaagacuc ugaagacgaa uauaucacac uagauaauau uuucggaaua      780

uuaauaggca aaugcacguu uuacccagaa gaauauaggg cagcgaaagc cucauacacu      840

gcacaagagu ucaaccucuu aaacgaucua aacaauuuaa ccguucccac ugaaacuaag      900

aaacucagcg aagaacagaa aaaucaaauc aucacaaacg uuaaaaauga aaaagcaaug      960

ggacccgcaa aacucuucaa auacaucgca aaacuacuau ccugugaugu agcagauaua     1020

aaaggcuauc gaaucgacaa aagugauaaa gcggaaauac acacuuuuga agccuaccga     1080

aaaaugaaaa cguuagcaac caucgauaua gaacaaaugg aacgagaaaa cuuagacaaa     1140

uuggcuuacg uccuaacacu aaauacugaa aaagaaggca uacaagaagc cuuagaacac     1200

gaauuugcag acggaaccuu uucacaagaa caaauagacg agcuaguuca auuccgaaaa     1260

gcaaauucau caauauucgg aaaaggaugg cauucauucu caguaaaacu aaugauggaa     1320

cuaauaccag aauuauaugc aacaagugag gaacaaauga caauuuuaac uagacuagga     1380

aaacagaaaa caacaucauc uaguaacaaa acaaaauaca ucgacgaaaa acaacuuacg     1440

gaagaaauuu auaacccagu agcagcaaaa ucaguacgcc aagcuauaaa aauaguaaac     1500

gcagccauca aagaauacgg agacuucgac aauauaguca uagaaauggc gagagaaacc     1560

aacgaagacg acgaaaagaa agcaauacag aaaauacaaa aagcaaauaa agaugaaaaa     1620

gaugcagcaa ugcuaaaagc agcaaaucaa uauaauggua aagcugaauu accacacagu     1680

guauuccaug gacauaaaca acuagccacg aaaauacgau uauggcacca acaaggagaa     1740

agaugcuuau acacuggaaa aacaauaucc auccaugacc uuauaaauaa cucaaaccaa     1800

uucgaaauug aucacauacu cccauuaagu auaaccuucg augacucacu agccaacaaa     1860

guccuaguuu augccacagc aaaccaagaa aaaggccaaa gaacaccaua ucaagcguug     1920

gacucaaugg acgaugcgug gagcuucaga gaacuaaaag cauucguuag agacucaaaa     1980

gcacuaagca auaaaaagaa agaauaccua cuuacagaag aagacauauc caaauuugac     2040

guuagaaaga aauuuaucga acgaaacuua guagacacaa gauacgccag caggguagua     2100

cuuaacgcgu uacaagaaca uuuuagagua cacaaaacag auacaaaagu aaguguaguc     2160

agaggacaau ucacaaguca gcuaagaaga cauuggggua uagagaaaac ucgagacaca     2220

uaucaccacc acgcaguaga ugcuuuaaua auagcagcuu ccucacaacu aaaccucugg     2280

aagaaacaga aaaacacucu agugucauau agcgaagacc aacuuuuaga uaucgaaaca     2340

ggagaacuaa ucucagauga cgaauacaaa gaguccguau uuaaagcacc auaucaacau     2400

uuuguagaua cacuaaaaag caaagaauuu gaagauucaa uacucuucuc cuaccaaguu     2460

gacagcaaau ucaauagaaa aauuucagac gccaccauau acgccacaag gcaagcuaaa     2520

guugguaaag acaagaaaga cgagacauac guacuaggaa aaaucaaaga cauauauagu     2580

caaacaggau acgacgcuuu cauaaaaaua uacaagaaag acaaaagcaa auuucuaaug     2640

uaccgacacg auccacaaac cuucgaaaaa guaauagaac caauacuaga aaauuauccu     2700

aauaaagagc ucaaugaaaa agggaaagaa guaccaugua auccauuccu caaauacaaa     2760

gaggaucacg gauacauaag gaaauacagu aagaaaggaa acgggcccga aauuaaauca     2820

cuuaaguacu acgauucaaa acuagguaau cacauagaua uuacuccuaa aaauucaaau     2880

aauaagguug uauuacaaag cguaucucca uggcgagcag acguauacuu uaacaaaaca     2940

acaggaaaau augaaaucuu aggacucaag uaugcagacc ugaaauuuga aaaagggaca     3000

gggacauaua aaauaagcga agaaaaauac aacgacauaa aaauaaaaga aggcguagau     3060

uccgauagug aguucaaauu uacacuuuac aaaaaugauc uacuauuaau caaggacacc     3120

gaaacaaaag aacaacaauu auucagauuc uuaucaagga cuaugccaaa cguaaaacau     3180

uauguugaac uaaagcccua ugauaaacaa aaguucgaug acaaugaaga acuaauuaaa     3240

auccucggga uaguagcaaa aggaggacaa ugcaagaaag gaguaucaaa accaaauauc     3300

ucuauauaca aaaucagaac ugacguauua ggcaaucagc acauaaucaa aaaugaaggu     3360

gacaaaccaa agcuggauuu uuga                                            3384


<210>  5126
<211>  3384
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5126
augaaugguu uaguccuugg ccuagacauc ggaauagcau caguaggagu aggaaucuua       60

gaaaaagacu cugguaaaau cauacacgca aauucgagaa ucuuccccgc agcaacagcg      120

gacaauaaug uugaaagacg uacaucccga caagcacgcc gacuuaaucg uagaaagaaa      180

caucgaagug uaagauuaca ugaccuuuuc gaagaauacg ggcuccuaac agauuuuuca      240

aaagugucaa ucaaccuaaa ccccuaucaa auacgaguac aaggacuaga caaaaggcuu      300

acuaacgaag aacucuucau ugcacugaaa aacauuguaa aaagaagggg cauaaguuac      360

cuagacgaug caucagaaga uggaggagcu guuaguucug acuauggaaa agcaguugaa      420

gaaaacagaa aacuauuagc ccaacaaacc ccaggacaaa uacaacugga cagauucgaa      480

aaauauggac aaguaagagg agacuuuaca guaguagaaa auggggaaaa acgaagacua      540

auaaaugugu ucagcacaag cgccuacaga aaagaagcag aaagaauacu uaggaaacaa      600

caagaauuua acaauaaaau aaccgaaggg uucauagaag auuacuugac aaucuuaacc      660

ggaaaacgca aauacuauca cggacccgga aaugagaaaa gcagaacuga cuauggaaga      720

uacaccacaa agaaagacuc ccaaggggaa uauauaacac uagauaauau uuucggcauu      780

uuaaucggga aaugcacauu cuacccugau gaauauagag cuagcaaagc aucauauaca      840

gcccaagaau uuaacuuauu gaacgacuua aauaaucuaa caguaccuac agaaacaaag      900

aaauuaagug uagaacaaaa gaaaaccaua guugaauacg caaaaacugc caaaacccua      960

ggggccucaa cgcuacuaaa auacauugcc aaacucauag gagucucugu agaccaaauc     1020

cacgguuaca ggauagaucc uaaugaaaaa ccagaaaugc acacuuucga aguauacaga     1080

aaaaugcaau cacuagaaac gauuagaguu gaagaauuac cgaggaaggu uauagaugaa     1140

cuugcucaca uauuaacauu aaauacagaa cgagaaggaa uugaagaggc cauaaaugca     1200

aaacugaaag auauauuuuc ucaagaucaa guucuagaau uaguacaauu caggaaaaac     1260

aauucuucgu uauucaguaa aggguggcau aacuucucac uaaaauuaau gauggaauua     1320

auaccggaac uguacgaaac aucugaggaa cagaugacaa uacuuacgag acuaggaaaa     1380

caaaaaucaa aagaaacguc caaaagaacg aaauacauug augagaaaga auuaacagag     1440

gaaauauaua acccagucgu agcaaaauca guuagacaag ccauuaagau aauaaaugaa     1500

gccacgaaaa gauacggaau auuugauaau aucguaauug aaauggcacg agaaaauaau     1560

gaagaggacg caaagaaaga auacauuaaa cgacaaaaag ccaacuuaga cgagaaaaac     1620

gcagcuaugg aaaaagcagc cuuucaauau aacggaaaga aagaauuacc cgauaacguc     1680

uuccacggcc auaaagaguu agcaacaaaa aucagguuau ggcaucaaca aggcgaaaaa     1740

ugccuguaua ccgguaaaaa cauaccaauc agcgaucuaa uacaaaaccg auacaaauac     1800

gaaauagauc acauacuacc gcucagccua ucauuugacg acucacuauc aaacaaaguu     1860

cucguauuag ccacugcaaa ucaagagaaa ggacaaagaa caccguuuca agcacuagac     1920

ucaauggauu augcauggag cuauagagag uucaaauccu acguuaaaga cucuaaauua     1980

uugucgaaca aaaagaaaga uuaccuauua acggaagaag auauaagcaa aauagaagug     2040

aaacaaaaau ucauugaaag aaaucuagua gacacaagau auuccagcag aguaguacug     2100

aaugcacuac aagacuuuua uaaagaacau caauucgaua ccacaauauc cguaguaaga     2160

ggccaauuua ccucucaacu aagaagaaaa ugggggauag aaaaaucccg cgagacauau     2220

caccaucacg ccgucgacgc cuugauaaua gcggcaucuu cacaacuaag auuauggaag     2280

aaacauaaua acccucuaau aaguuacaaa gaggaacaau uugucgauuc agaaacuggg     2340

gaaauaaucu caaugucaga ugacgaauau aaagaguuag uauucaaagc cccauaugac     2400

cacuucguag auacacuaaa aucaaagaaa uuugaggacu ccauacuauu cucauaccaa     2460

guagacagua aauacaaucg caaaauuuca gaugcuacaa uauacgcaac uagaaaagca     2520

aaacuagaua aagauaaaag ugaagaaaca uacguacuag gaaaaauaaa agauauauau     2580

agucaagccg guuacgauac cuucauaaaa auauacaaua aggauaaguc cagauuccua     2640

auguaucaca aagauccgca aacauucgaa aaagucaucg aagaaauacu uagaaccuau     2700

ccaucaaaag aacuaaacga gaaaaauaag gaagucccuu gcaaccccuu cgaaaaauac     2760

agacaagaaa acggaccgau aaggaaguau agccgaaaag gaaacggacc cgaaaucaaa     2820

ucacuaaaau auuaugauaa uaaacuagga aaucacauag auauuacacc caaugaaucu     2880

augaaccagg uuguccuuca aucuuugaaa ccuuggagaa cggacguauu uuucaaucac     2940

caaacggaaa aauaugaacu aaugggauua aaauauagug accucaauuu cgagaaagga     3000

agcggcaaau acucaauaag uuuagaaaaa uacaacagca uaaaaaugaa ugaaggcguc     3060

ucagaagaaa gugaguucaa auucacauua uauaaaaaug aucuuauacu aauaaaagau     3120

gcacucacaa aagaagcaca aauauuuaga uacggcagca ggaacgacac auccaaacac     3180

uacguugaac uaaagcccua ugauauguca aaguucgaag gagacgaaag acuaauaaaa     3240

guauuaggaa caguagcaaa aggaggaagg uguauaaaag gacucaacaa gaaagacaua     3300

ucuauauuua aagugaaaac ggacguacua ggcaauaaac acuuuauaaa gaaagaaggc     3360

gaagaaccua aauuaaaauu cuga                                            3384


<210>  5127
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5127
augacaaaac uuggcaaacc uuacgggaua ggaguugaua uaggaucgaa cucaauuggu       60

uucgcagcag uagacgaaaa uucacauuua auacgucuaa aagguaaaac uguuaucgga      120

gcucgauuau ucgaagaggg uaaagcggca gcaaaacgca gagcagguag aacaaccaga      180

agacgauuau caagaaaucg cuggcgucuc agcuucuuaa gagacuuuuu cgagucacau      240

aucacuccua cagauccaaa cuuuuucaug agacaaaaau auagugagau cagcccaaaa      300

gauaaagcac gauacaaaua cgaaaaacga cuauuuaaug auagaaccga cgcugaauuu      360

uaucaacaau auagcacuau guaccaccua cgaaaucgac uaaugacaga uccuagcaga      420

gcagacguac gagagauaua cuucgcaaua caucauauac uaaaaaguag aggacauuuu      480

cuaacgccug gagaugcaaa agauuucaac acgaacgacg uagcacuaga ugaaauauuc      540

cccgcgcucc aagacgcaua ugcacaaguc uaucccgacc uagggauuac cuucgacgag      600

gauaaagcua augaguucaa aacgauacug uuaaacgaaa uagcuacauc aaucgacacc      660

caaagagcuu uaguaaaacu gcuucuagca gaagaagaug acaaagauau acuaaaacaa      720

caaaaacaag uucuuacuga guucgcaaaa gcuguagucg gacuuaaaac aaaacugaau      780

cuugcacucg gcacugaagu agacucaucu gaagcaacag cauggaauuu uucuuuaggc      840

caauuagacg auaaaugggc uggaauagaa ucagcuauga cagacgaagg aaccgaaaua      900

uuagaccaaa uuagagaccu auacagagcc agauuacuca acggaauugu uccaaccgga      960

aaaacauuaa gucaagcaaa gguagacgau uacacacaac aucacgaaga cuuacaacua     1020

uuuaaggcau accuaaaaca acuagaagac gauggcacag caaaagcuau aaggcaacua     1080

uaugauagau auauagacgg agaugaugca gcaccguucu uaagagaaaa cuucguuaaa     1140

gcacugacca aggauguaac ugcccaccca aauacaaaaa guccagaacu auuagaaaga     1200

uuagcucaac cugauuuuau gcuuaaacaa agaaacaaag cgaacggggc aauaccaguc     1260

caaaugcaac aaagagaauu agaccaaaua auuaaaaauc aagccguaua cuaugacugg     1320

uuagccgcac caaauccagu cgaaaaacau cgaaaaucua ugccauauca auuagacgaa     1380

cuacuaaauu ucagaauacc auauuacguu ggaccacucg uaacagcaaa agaacaaaag     1440

gcagcacaag gcggaguauu cgcauggaug guaagaaaag auccagaagg aaauauaaca     1500

ccauauaauu uugacgaaaa aguagaccga gaagccagcg ccaacaccuu cauccaaaga     1560

augaaaacca cagauacaua ccugauaggu gaagauguac uaccuaaaca aucucuacuc     1620

uaucaaagau acgaaguacu uaacgaacua aacaauguaa gaguaaacaa ugaaaaauua     1680

aguaucgaac aaaaacaaca aguuauaaga gaacuauucg aacgccacaa uucuguaacu     1740

aucaaacagu ucgcagaaaa ucucagagca cauggagacu augcacauau accagaaaua     1800

agagguuuag cagaugaaaa acgauucuua ucaucacucu caacauaucg acaacuaaaa     1860

ucucugcugc cagaagcaau agacgauccu gcaaaacaag cggauauuga aaacauaaua     1920

gcauggucaa caguauucga agacgcagcu auauuuaaaa cgaaauuaaa agaaauaagu     1980

uggcuaggau cagaagccau aacaaaauua agcaauauac guuaucgggg auggggucaa     2040

uuuucccgaa aauuccuuaa uggucugaca cuaggcaaug gacacaccau aauucaagaa     2100

uuacugcuaa gcaccaacaa cuuaaugcaa auuuuaacgg acgaaacacu acaaaagaaa     2160

augacagaac ucaaugcaga caaauuaaaa acagcuaaua uaaacgaugc aaucgauaau     2220

gccuacacuu cacccagcaa uaagaaagca cuccgucagg uguuacgagu aguagaugau     2280

auaaaaagag cagcagaugg acaagaucca ucauggcuau acguagaaac ugcagauggc     2340

ggcggcacuc cugguaaaag aacuagagca agacaacacc aguuacaaga aauuuacgca     2400

aacgcugcac acgaacuaau ugauacagca guccguggag aacuggaaga uaaaaucucg     2460

gauaaagcag auuucaacga cagauuaguc cuauauuuca ugcaaggggg aagggauauc     2520

uacacaggag cgccacuuaa cauagaccaa cuuucgaguu augacauuga ccacauucua     2580

ccacagagcu uaauaaaaga caauagucua gacaaucgag uucuaguaca cgccauaaua     2640

aauagagaga aaaacgcuac auuugcaagu acaaucuacg cacaaaagau gaacgcaacu     2700

uggaggcaau ggcaugaagc aggacuaaua aguggaagaa aacuaagaaa ucuucaaaug     2760

agaccagacc aaauagacaa guaugcaucu ggauuuguug cucggcagcu aguagaaacc     2820

agacaaauaa ucaaacuaac agaacaaaua guagcagcac aauacccuga cacuaaaaua     2880

auugcuguaa aagccgggcu cagccaccaa uuaagagaag aauuagaauu ucccaaaaac     2940

agagacguaa accacuacca ucaugcauuu gacgccuuuc uagcagcccg caucggcaca     3000

uauuuauuaa aaagauuucc aaaucuccaa gcauuuuuca cauaugguaa auucacaaaa     3060

gcagacguca aaaaguuaag aggguuuaau uucauccgug auauaacaca ugcagaagau     3120

aaaauaguag caaaagauac aggugaaguc guuugggaca aacaaagaga cguagaugaa     3180

uuagacagaa uauacaacuu caagagaaug cuaauaacgc acgaaguaag auucgaaaca     3240

gcggaucuau ucaagcaaac aguauaugga gcuagggacu cuaaagaagc aggaggcuca     3300

aaacaacuaa ucccaaagaa gaaagauauu caacuaaucu ucaugggugc aauaagugag     3360

agaacuagac cuauacugcc uuuguga                                         3387


<210>  5128
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5128
auggaugaaa aaauggauua uagaauaggc cuugacaucg gaauagcauc aguaggaugg       60

gcaguuuuac aaaacaauag ugacgacgaa ccaguaagaa uaguagauuu aggaguuagg      120

aucuuugaua cagcagagau accuaaaacu ggugagucac uagcagggcc aagaagagcu      180

gcaagaacca cgcgccgaag auuaagaaga agaaaacacc gacuggacag aauaaaaugg      240

cucuucgaaa aucaaggucu aauaaauaua gaugacuuuu uaaaacgaua uaauauggca      300

ggacuaccag acguauauca auuaagauac gaagcacuag auagaaaacu aaccgacgaa      360

gaacuugcac agguauuauu gcacauagcu aaacacagag gguuuagguc aacgcgaaaa      420

gcagaaacag cagcuaaaga aaacggugca guauuaaaag caacagacga aaaccaaaaa      480

agaaugcagg aaaaaggaua uagaacaguc ggagaaauga uauaucugga cgaagcuuuu      540

cgaaucgguu gcagcuggag ugaaaaaggu uacauauuaa cuccaaggaa caaagcagaa      600

aacuaucagc acacaaugcu gagagcaaug cuaguagaag aaguuaaaga aauauucucu      660

agucaaagaa gacuagggaa caaaaaagca acugaagaac uagaagaaaa auaccucgaa      720

aucaugacau cacaacgaag uuuugaccua ggcccaggaa ugcaaccaga cggaaaaccg      780

ucaccauacg caauggaagg uuuuucagau agaguaggca aauguaccuu cuuaggugac      840

caaggggaau uaagaggggc aaaaggaaca uacacagcag aauacuucgu ugcgcuucaa      900

aaaauaaacc acaccaaauu agucaaucaa gacggagaaa caagaaacuu cacagaagaa      960

gaaagacgag cgcuuacccu acuacuauuu acccaaaaag aaguuaagua ugcagcagua     1020

cggaaaaaac uaggauugcc ugaggauaua cuauucuaua aucuaaacua caaaaaagcg     1080

gcaacaaaag aagaacaaca aaaggaaaau caaaacacag aaaaagcuaa auucauagga     1140

augccguauu aucacgacua caaaaaaugc cuagaagaaa gaguaaaaua ccuuacugag     1200

aaugaaguca gagaccuauu ugaugaaaua ggcaugauac uaacauguua caaaaaugac     1260

gauucaagga cagaaaggcu agcuaaacua ggucugguac caauagagau ggaaggguua     1320

cuagcauaua caccuacaaa auuccaacau uuaagcauga aagcaaugcg aaauauaaua     1380

ccauuccuag aaaaaggaau gacauacgau aaagcaugug aggaggcagg uuaugauuuc     1440

aaagcagacu cgaagggaac aaagcaaaag cuauuaacag gggaaaaugu uaaccaaaca     1500

aucaaugaaa uaacaaaucc aguaguaaaa cgaucaguau cucaaacugu aaaaguaaua     1560

aacgcaauaa uaagaacuua cggaaguccu caagcaauca auauugaauu ggcaagagaa     1620

augucgaaga cauucgaaga acgaaggaaa auaaaagggg acauggaaaa aagacaaaaa     1680

aacaaugaag acgucaaaaa gcagauacaa gaacuaggaa aauuaucacc aacaggccaa     1740

gacaucuuga aauacaggcu cuggcaagag caacaaggaa uauguaugua uagcggaaag     1800

acuauuccgu uagaagaauu guucaaaccg ggcuaugaca uagaucauau acuacccuau     1860

uccauaacau uugaugacag cuucagaaau aaaguauuag uaacaagcca agaaaacaga     1920

caaaagggaa accgaacacc auaugaauau augggaaaug acgaacaaag auggaaugaa     1980

uuugaaacuc gugugaaaac gacaauaagg gauuacaaga aacaacagaa auuacuuaaa     2040

aaacacuuca gcgaagaaga acgaagcgaa uucaaagaac gaaauuuaac cgacacuaaa     2100

uauaucacca cugugauaua caacaugaua agacaaaacu uggaaauggc uccauugaac     2160

cgaccagaaa aaaaaaagca agugagggcu gucaaugggg caauaacagc auauuugcga     2220

aaaagguggg gacuuccuca aaaaaaccga gaaacagaua cacaucaugc cauggacgca     2280

guugucauag cuuguugcac agauggaaug auccaaaaaa uuaguagaua uacaaaaguc     2340

cgagagcgau guuauucuaa aggaacagaa uuuguagaug cugaaacugg cgaaauauuc     2400

cgaccggaag auuacucuag agcagaaugg gacgaaauuu ucggagugca uauaccgaaa     2460

ccaugggaaa cauuccgugc ugaacuagau guccgaaugg gagaugaccc aaaaggauuu     2520

uuagacacac auucagacgu agcccuagaa uuagacuacc cagaauauau uuacgaaaau     2580

cuacgaccca uauucguauc aaggaugcca aaucauaaag uaaccggugc ugcucaugca     2640

gacacaaucc gguccccaag gcacuuuaaa gaugaaggga uuguacucac aaaaacagca     2700

uuaacggauu uaaaacugga caaagacggu gagauugaug gcuauuacaa uccucaaucu     2760

gaccuacuuc uauacgaagc acuuaagaag caauuacuau uauaugguaa cgacgcaaaa     2820

aaagcuuucg cucaagacuu ucauaaaccu aaagcagacg guaccgaggg uccaguuguc     2880

cguaaaguca aaauucaaaa aaaacaaacg augggaguau uugucgacag uggaaacgga     2940

auagcggaaa auggcgguau gguccguaua gacguauuuc guguuaaugg aaaguauuau     3000

uuuguaccug uuuacacagc ugaugucgua aaaaaaguuu uaccgaaccg ugcgaguaca     3060

gcaaauaaac cuuauaguga auggagaaca auggaagaua aagauuuccu auucagcuug     3120

uauagucgag auuuaguaca cauuaaauca aaaaaaggca uaccuauaaa aaaugucgac     3180

ggaucucucg gagaaaguaa agaaguuuac gccuauuaua uaggagcaga caucucuggg     3240

gcaaaaauag agggaaaagc acaugauagu agauauacau uuagaggaau cggaguacaa     3300

aaacucgaau uaauagaaaa gugccaagua gauauauuag gacacauauc aacagucaag     3360

aacgaaaaac gcaugggcuu cucauga                                         3387


<210>  5129
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5129
augaacgaua aaaaucaaaa auauagauua ggucuagauu uagguacaaa uuccauagga       60

uggugcauau acucauuaga uguaaacaac aaacccgaaa cucuuauaga caugggaguu      120

cgaauauuca gugacggcag agacccaaaa acaaaagaac cacuugcugu agaaagaaga      180

acagcaagag ggcuuagaag aauacuucau cgcagaaaac uaaggagaag acaaauguuu      240

agacuuuuac aagaacaagg acucaugccc aaugaugcaa gugaagcagc ggcauuaaaa      300

ucacuaaauc cauacgaacu aagaguaaaa gcauuggaua ccaagcuaga accauaugaa      360

uuaggccgug cuuuauuuaa ucucucagua agaagaggcu uuaaaucaaa ucgaaaagaa      420

gcgguccaag aaaaagaaaa agacucccaa aacgacgcua agagucaaaa ugaaaaaugc      480

gaacauuuag caaaagcaau caaagauagu ggguguagaa cacuaggcga guuccuaugg      540

aaacauaaaa acacugaaac ucaacaaaca gaaaaugacg caagaaacau aggaaugcgc      600

uuuguaccgg guagaaguaa cuacuauccc acgagacgaa uguacguaga agaauuugau      660

gcuauaagaa aagcccaaga acccuacuac ucagggguaa acugggacgc ucuccacgaa      720

aagcuauuuu aucaacgacc gcuuagagcc caagaacgag gcaaaugcag auauaugcca      780

gacaaagaga gaacauuuaa agcaaugcca ugcucacaga aaauaaggau acugcaagaa      840

guauacaaca uggauuauau ugacgaauac ggaaaaacua uaccuuuaga agaagcacaa      900

acagacagcc uuaucagucu ucuagacaaa agagaaaaac uauccuucaa agcaaugaag      960

aaagaauuag gaauagacga aucauguaca uuuaaucugg agagaggagg ucgagaauac     1020

uuacaaggaa acgccgcagc cguaaaacuu agaaauaaga aacguuucgg agaacuaugg     1080

gauucacuaa gucucgccaa acaagacgca auaguagaaa aacucauaac agcugaugaa     1140

gacgccgaaa uaauaagcau guuagcacaa uacaacuuaa cagaugaaca aaagaaaaac     1200

auagccguaa cuguacugcc cucaggaacg gccaugcuau gcaaagaagu gacagagaug     1260

cuuguucaaa agauggaaca aaauaaaauu ccacucacag uugcaguaca aaugcuggga     1320

uacaccuacg cugaucaauc aguaagagaa gaagacacau uaccauauua cggagcaauu     1380

cucaccggga gcacaauggg ugcucaccca gaagcagacg aaucagaacc ggaacuaaaa     1440

uacggaaaaa uaagcaaucc uacaguacac guagcacuua aucaaacuag aacaguugua     1500

aaugcacuaa uuaaagcaua cggaaaacca caacaaauag uagucgaacu cucaagagau     1560

uuaaaagcau caagggaagc aaaagcaaga auacagaaaa cuauaaccau gaaccagaaa     1620

aggaacgaaa gagacaacaa aaauauaacu gauauaacaa aaauccccua cccaaacaga     1680

aacgacagac uaaaauaucg acuuugggaa gaauugggau caggaucuaa cagcuuuagu     1740

agaaaaugcc uauauugcgg aaaaccgaua ucuggaucag aaauuuucac agaaaauaua     1800

gagauagaac auauuuuacc cuucggacga acacuauucg acggagagac gaacaaaacu     1860

guagcacaca cugacugcaa uagagauaaa ggagaccgau caccauucga agcauuugga     1920

agcucaccga auggauacaa cuggcaugaa auaugugcac gggcaaacuc ccuuaaaaac     1980

ccagcuaaac guacguuauu cgccgagaac gccauggaaa aauuugaaaa agacaacuca     2040

uucauacaac gacaacuaac agauaacgca uaccuaucaa gaagcgcacu cagguauuua     2100

cgaugcauuu guaaagacgu cuggucaaua aauggaggua ugacuaaacu ucuacgcgac     2160

aaauggaaua uagauuccau auugaaaaga aaaauagaug aucccgaaau agcacauuuc     2220

gaacuuaaaa augaccaaau aggucaauac aagaaaaaua gguaugauca cagacaccau     2280

gccuuagacg cgucaguaau agcauuaaua gacagagcua ugguacaaga aaucucacaa     2340

cuaaacaaaa uaagguugaa aaaccgaaua gaagcaccac aaaugccagu acuaaggucg     2400

gaacuuaucg agaaaguaaa acacaucacc guaaguuuua aaccagauca uggccggcaa     2460

gguaaacuau caaaagaaac acuacuagga aaaauuaaaa agaucgaaaa aguggacauu     2520

gauagcauac aaaccgacga cauagcuuca aucaaagaug augcaauaag aacccgauuu     2580

gaaacaaaaa gaagagaauu aggagauaua acaaaagcaa aagcagcauu aaaaacagaa     2640

uuuccaaaac uugauguauu uaagacauau uauguuucac gcacgcccau agucagucua     2700

acagagaaaa auauaaauuc aauaguagau gagaaaauaa aagaaaaacu caaaacuuuc     2760

auuaaagaac acgccgauuu aucauuugca gaacaacuac aaaaauucuc ugaaacagaa     2820

uggauaggca aaccgucggc cgcaggaaaa ccagguaaga aauauagaau agagaaaguc     2880

agaugcauaa acuggggcca aacaccaaua acaauaacaa guagugcagu accccgguau     2940

cuaugcccag aagacuaucu cgcugcagua guuuggcaaa uaccgaguaa aaagaaagac     3000

acagaacccg aauauaaagc aacauuuuua agacgagaug aguucgaugu aaauaacaaa     3060

ccaaagaaac uagaaaaacc acacgaugcc gcacaauaca uaugcauguu auacaaagau     3120

gacuaccuug aguucacaga aaguggagca acacauuugu guagaauagc aggccuacca     3180

gcuucacaaa auaauaaaau ugauauucgu ccaauauacg ccguaucuga cuguaaagau     3240

uggauaauag ccacaaauga aaacauguua gaaccauguu ggaaaccaca aaaagaagaa     3300

aaacgaauaa guguuaaugu auuauucgga aagcauaaag cuagauccgu aacggugaac     3360

cccaucggac gggucuuccg aaaauga                                         3387


<210>  5130
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5130
augucagauu uaguucuggg acuggacaua ggaauaggau caguaggagu cgguauacuc       60

aacaaaguaa caggagaaau aauacacaaa aacucacgaa uauuuccggc agcucaagca      120

gaaaauaacg uagaaaggag aacaaauaga caagguagaa gacuaaccag acgaaagaaa      180

cacagaagag uaagacuuaa ccaucucuuu gaagaauccg gacuaauaac ggacuucacc      240

aaaguaucaa uaaaccuuaa cccauaccaa uuacgaguca aaggacuaau agacgaauug      300

aguaacgaag aauuauucau agcuuuaaag aacaugguga aacauagggg gauaaguuac      360

uuagacgacg caucagacga ugguaauucc ucaguaggag acuaugcaca gaucguuaaa      420

gaaaauucca aacaacuaga aacaaaaaca ccaggacaaa uccaacuaga aagguaucaa      480

aaauaugguc aauuaagagg agacuuuacc guagaagaag acggaaagaa acacagacuu      540

auaaaugugu ucccuaccag ugcauacaga ucugaagcau uaaggauacu gcaaacacag      600

aaagaauuua auccucaaau cacagacgag uucauaaauc gauaccuaga aauuuuaacc      660

ggaaaacgca aauacuacca cggaccagga aacgaaaaau ccagaaccga uuauggacga      720

uauacaacaa agaaagauuc agaaaaugaa uauauaacuc ucgauaacau auucgguaua      780

cuaaucggca aaugcacauu uuacccugaa gaauauagag cagcaaaagc cucuuauaca      840

gcacaagagu ucaacuugcu aaacgauuua aacaaccuca cagucccaac agaaaccaag      900

aaacuaagcg aagaacagaa aaaucaaaua auaaauuaug uuaaaaauga aaaagcaaug      960

ggccccgcaa aauuauucaa auacauagca aaauuacuua guugcgacgu agcagacaua     1020

aaagguuaua gaauagacaa auccgacaaa gccgaaaucc acaccuucga agcauacaga     1080

aaaaugaaaa cucuagaaac cauagacuuc gaaaagauga gucgcgauca auuagauaaa     1140

uuagcauacg uacuaacucu aaauacagaa agagaaggaa uccaagaagc cuuagaccac     1200

gaauuugcag auggaaacuu cucacaagaa caaauagacg agcuagucca auuccggaaa     1260

gccaauucuu ccauauucgg caaaggaugg cauaacuucu cuguaaaauu gaugauggaa     1320

cuuauuccag aacuauacgc aacaucagag gaacaaauga caauacucac aagauuagga     1380

aaacaaaaga caacuucuuc aucaaacaaa accaaauaca uugaugaaaa acuuuuaaca     1440

gaagaaauuu acaaccccgu aguggcaaaa ucuguaagac aagcuauaaa aauaguaaau     1500

gcagcaauaa aagaauaugg ugacuuugac aacauaguca uagagauggc cagagaaacu     1560

aacgaagaug acgaaaagaa agccauacag aaaauacaaa aagccaacaa agaugagaaa     1620

gacgcagcca ugcuaaaagc agcgaaccaa uacaauggac gagcugaacu accacauuca     1680

guauuucacg gacauaaaca auuagcuaca aaaauaaggu uauggcauca acaaggagaa     1740

cgaugcuuau auaccgguaa aacuauuagu auucaugacc uaauaaauaa cccgaaccaa     1800

uucgaaauag accauauauu accuuuaagc auaacauuug acgauucacu cgcuaacaaa     1860

guccuuguau acgcaacagc aaaucaagaa aaaggucaaa gaacaccaua ccaagcauug     1920

gacuccaugg augaugcuug guccuuucgc gaguuaaagg cuuucguaag agaaagcaaa     1980

agccucagca auaaaaagaa agaauaucua uuaacagaag aggacaucuc aagauucgac     2040

guacgaaaga aauucauaga gaggaaccua guagacacaa gauacgcguc acgaguugua     2100

uuaaaugcac uacaagaaca uuuccgcgca cacaaaacgg acacaaaagu cuccguuguc     2160

agaggacaau ucacaagcca acugcgaaga cacuggggaa uagagaaaac cagagauacu     2220

uaucaccauc augcagucga cgccuuaauc auagcagcua gcucacaauu aaaccuuugg     2280

aagaaacaga aaaauacucu aguaaacuau agugaaaauc aacuacuaga uaucgaaaca     2340

ggagaauuaa uaucagauga ugaauacaaa gaaucaguau ucaaagcacc auaucaacau     2400

uuuguagaua cacuaaaauc caaagaguuc gaagauagca uacuauucuc auaucaagua     2460

gacucaaaau uuaaccgaaa aauaagugau gcuacaaucu acgcaaccag acaagcaaaa     2520

guaggaaaag acaagaaaga cgaaacauau guauuaggaa aaaucaaaga cauauacuca     2580

caaacaggau acgaugcauu uaucaaaauc uaccaaaaag auaaaucaaa auuuuuaaug     2640

uaccgccacg acccacaaac uuuugaaaaa guuauagaac caauacuaga aaauuaucca     2700

aauaaagaaa ugaacgaaaa aggcaaagaa guccccugca acccauucuu aaaauauaag     2760

gaagaacacg gagacuauau ucgaaaauac agcaagaaag gaaacggacc cgaaaucaaa     2820

ucccuaaaau acuaugauag uaaauuagga aaccauauug acauuacucc caaagacuca     2880

aacaauaagg ucgugcuuca aucagugagc ccauggagag cagacguaua cuucaacaag     2940

acaacaggua aauacgaaau auuaggauua aaauacgcag aucuucaauu cgaaaaagga     3000

acaggaacau auaagauaag ccaagaaaaa uacaauguaa uuaagaagaa agaaggaguc     3060

gacucagacu cugaauuuaa auuuacacuc uauaaaaacg aucucuuauu aauaaaagac     3120

accgaaacaa aagaacaaca auuauuuaga uuccuaucaa gaaccaaacc caacguuaaa     3180

cacuauguag aauuaaaacc uuacgacaag caaaaauucg aaggcaacga aagccucaua     3240

aauguguuag gagcaguagc caaaggcggc caaugucaaa aagguauaaa caaaccaaac     3300

auuagcauau acaagguaag gacugacguc cuaggaaacc aacauauaau caaaaacgaa     3360

ggagauaaac cuaaacugga uuucuga                                         3387


<210>  5131
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5131
auggaugaga aaauggacua uaggaucgga cuagauaucg gaauugcuag cgugggaugg       60

gccguccuac aaaauaauuc agaugaugaa ccugucagaa uaguggaucu aggaguuagg      120

auauucgaua cagcggaaau accaaaaacu ggugaaucac uugccggacc ccgaagggcu      180

gcaaggacca cacgaaggag acuaagaaga agaaaacaua gacuugaccg gaucaaaugg      240

uuauucgaaa accaaggacu aaucaacauu gaugacuucc uaaaaagaua caauauggca      300

ggccuuccag auguauauca acuaagguac gaagccuuag aucgaaaauu aacagacgaa      360

gaacuagcac aaguuuuacu acauaucgca aaacaccgag gauuuaggag cacacgcaaa      420

gcagaaacag cagcuaaaga aaauggagcg guguugaaag caaccgauga aaaccagaaa      480

agaaugcaag aaaaaggaua uaggacagua ggugaaauga uuuacuuaga cgaagccuuu      540

aggacaggau gcucaugguc ggaaaaaggu uauauucuaa ccccaagaaa uaaagccgaa      600

aauuaccaac acaccaugcu aagggcuaug cuagucgaag aaguaaaaga gauauucagc      660

ucacaaagga gacuagguaa ugaaaaagcg acagaagaau uagaggagaa auaucuugaa      720

auaaugacau cacaaaggag uuuugauuug gguccaggga ugcagccaga cgggaaaccg      780

agcccauacg cgauggaagg guuuagugac aggguuggaa aaugcacauu ucuaggagac      840

caaggggaau uaagaggagc aaaaggaacu uacacagcug aguacuuugu agcauuacag      900

aaaauaaacc auacaaaacu ugucaaccaa gauggagaaa caagaaauuu cacagaagaa      960

gaaagaaggg cguuaacauu acuguuauuc acacaaaaag aagugaaaua cgccgcugug     1020

agaaagaaau uggggcuacc ugaagauaua uuauuuuaca accuaaauua caagaaagcc     1080

gccacgaaag aagaacaaca gaaagaaaau caaaacacug aaaaagcuaa auucauagga     1140

augccauauu aucacgauua caagaaaugc uuagaagaaa gaguaaaaua ucuaaccgag     1200

aaugaaguaa gagaucucuu ugaugaaaua ggaacaaucc ucacauguua uaaaaacgau     1260

gauucgagaa cugaaagauu agcaaaacuu ggucuaguuc caauugagau ggaaggauua     1320

cuagcauaca caccaacaaa auuccaacau uuguccauga aagcuaugag aaauauaaua     1380

ccuuucuugg aaaaaggaau gacauaugac aaagcaugcg aagaggcagg cuacgauuuu     1440

aaagcagauu ccaaaggaau aaaacagaaa cucuuaaccg gggaaaaagu gaaccaaacg     1500

auaaaugaaa uaacaaaccc aguugucaaa agaucaguau cccaaaccgu caaaguuaua     1560

aaugcuauaa uaagaacgua cggaagcccg caagcaauua auauagagcu agcaagagaa     1620

augucaaaaa cuuuugaaga aaggcgaaaa auaaaaggcg acauggaaaa acggcagaaa     1680

aacaaugaag auguaaagaa acaaauacag gaauuaggaa aauuaucacc uacuggucaa     1740

gauauacuca aauacagacu cuggcaagaa caacagggaa uuuguaugua uucagguaaa     1800

acuauaccac uagaggaacu auuuaaaccu gguuacgaca uagaccacau cuuaccauau     1860

ucaauaacuu ucgacgauag uuuuagaaac aaaguacuug ugacaaguca agaaaacagg     1920

caaaaaggaa auagaacacc auaugaauau augggaaacg augagcaacg auggaacgaa     1980

uuugaaacaa ggguaaaaac aacaauaaga gauuauaaga aacaacaaaa acuacuuaag     2040

aaacauuuuu cagaagaaga acgcuccgag uucaaagaaa gaaaccugac ugauacuaaa     2100

uacaucacaa ccguaauaua uaacaugaua aggcagaacu uagaaauggc accauuaaac     2160

agaccugaga aaaagaaaca agucagagca gucaauggcg caaucaccgc uuaccuacga     2220

aaaagguggg gccucccaca gaaaaacaga gaaacagaca cacaccaugc uauggaugcg     2280

guaguuauag cauguuguac agaugguaug auacagaaaa uaagcagaua uacaaaaguu     2340

agagaaagau guuauagcaa agguacagaa uuuguagaug cggaaacagg cgaaauauuu     2400

cgccccgaag auuacagcag agcagaaugg gaugaaauuu ucggcguaca uauaccaaaa     2460

ccuugggaaa cauuuagggc agaguuagau guaaggaugg gugacgaucc aaaaggauuu     2520

cuagacacac acagcgaugu ugcacuagag cuagacuauc cagaauauau auaugaaaac     2580

uuacgaccaa uauucguguc aagaaugcca aaccacaaag uaacuggugc agcacaugca     2640

gauacuauac guagcccacg ucauuuuaaa gacgaaggua uaguacugac aaaaacagcg     2700

uuaacagauu ugaaacugga uaaagauggg gaaauagacg guuacuauaa cccgcaauca     2760

gacuuacuuc uuuaugaggc auuaaagaaa caauugcuau uauaugggaa ugacgcaaag     2820

aaagcauuug cacaagauuu ucacaaaccg aaagcagaug gaacagaagg cccaguagua     2880

cguaaaguua aaauacagaa gaaacaaacc augggagugu uuguugacag cggaaauggc     2940

aucgcugaaa acggaggaau ggugagaaua gaugucuucc augugaacgg uaaauacuac     3000

uuugugccag uauacacagc agacgucgua aagaaaguac uuccuaauag agccgcuaca     3060

gcacauaaac cauauggaga auggaaagua auggaagaca aagauuuucu uuuuagucua     3120

uacagccgag acuuaauaca uauaaaaagc aagaaagaca ucccgauaaa aauggucaac     3180

ggugguaugg aggguaucaa agaaacuuac gcguauuaua uuggcgccga uauaucagcu     3240

gcaaauauuc aagggauagc acaugacucu agauauaaau ucagaggccu ugggauucaa     3300

agccuagaug uuuuagaaaa augucaaauu gacguacuug gacacguuuc cguaguaagg     3360

agugagaaaa gaaugggauu uucauga                                         3387


<210>  5132
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5132
augucagacc uaguccuugg ucucgauaua ggaauaggaa gcguuggugu agguaucuug       60

aacaaaguca cuggagaaau cguucauaaa aacgcaagaa uauucccggc cgcacaagca      120

gaaaauaaug uagaaagaag gaccaaucga caaggaagac guuuaacgcg aagaaagaaa      180

cauagaagag uacguuuaaa ucauaucuuc gaggaaagug gauuaauuac agacuuuaca      240

aaaguuucaa uaaaccuaaa uccauaccaa uuacgaguca aaggccuaac agacgaacua      300

ucuaaugaag aauuauucau agcucugaaa aauaugguaa aacaucgagg aauaucuuau      360

cuugaugaug caucagauga uggaaauagc agcguaggag auuaugccca aauuguuaaa      420

gaaaauagua aacaguuaga aaccaaaaca ccaggccaaa uacaacuaga acgauaccag      480

aaauaugguc aacuacgagg ggauuuuacc aucgaagaag acggaaagaa acauagauua      540

auaaauguau ucccaacguc agcauaucgg gcagaagcau uaaggauacu acaaacacag      600

caagaguuca auccucaaau aacagacgag uucauaaaca guuacuugca gauccuaacc      660

ggcaaaagaa aauauuacca uggcccuggc aaugaaaaau ccagaacuga uuacggacga      720

uacaccacca agaaagacag cgaagacgaa uauaucacuc uggacaacau uuucggcaua      780

cucauaggca aauguacauu uuauccagaa gaauaccgag cagcuaaagc aaguuauacg      840

gcacaagagu ucaaucuccu aaacgauuua aacaaccuca ccguaccaac agaaacgaag      900

aaacuauccg aagaacagaa aaaucaaauc auaacauaug uuaaaaacga aaaagcaaug      960

ggcccagcaa aacucuucaa auauauugca aaacucuuag ccugcgacgu agcagacauc     1020

aaaggauacc guauagauaa aucagacaaa ccagaaauac acacauuuga agccuacaga     1080

aaaaugaaaa cccucgaaac cauagauguc gaacaaaugg aacgagaaaa acuagacaaa     1140

cuagcauacg uuuuaacacu aaacacugaa cgagaaggua uucaagaagc cauagaacac     1200

gaguucucgg acggaacauu uucacaagaa caagucgacg aauuagugca auuucgaaaa     1260

ucaaauagcu ccauuuuugg aaaaggaugg cauaacuuuu caguaaaauu aaugauggaa     1320

uuaauaccag aacuauaugc uacaucggaa gaacaaauga caauucuaac aagacuugga     1380

aaacaaaaac gaaccaguuc aucaaacaaa acuaaauaca uugaugagaa aagguuaaca     1440

gaagaaauau acaauccagu aguugcaaaa ucuguaagac aagcaauaaa aaucguuaau     1500

gcagcaauua aagaauaugg agacuucgau aacauaguaa ucgaaauggc acgcgaaaug     1560

aacgaagacg augaaaagaa agccaucaag aaaauacaag aagccaauaa aucagaaaaa     1620

gacgcagcua ugaggaaagc agcaaaccaa uauaauggaa aagcagaacu accacauucc     1680

guauuccaug gccacaaaca auuagcaacg aaaaucagau uauggcacca acaaggagaa     1740

cgaugccuuu acacgggaaa aacgaucagu auacacgacc uaauaaacaa uccaaaucaa     1800

uuugaaauag accacauccu acccuuauca auuacauuug augauagucu ugccaauaaa     1860

guauuaguau augcaacagc aaaucaagaa aaaggacaaa gaaccccuua ucaagcauua     1920

gacuccaugg acgacgcaug gucauucaga gaacuaaaag cauucguaag ggaaucaaaa     1980

accuuaagua acaaaaagaa agaauaccuc uugacggaag aagacauauc aaaauucgac     2040

guccgaaaga aauucauaga aagaaaccua guugauaccc gauacgccuc acgaguagua     2100

cuuaaugcac uacaagaaca cuuuagagca agaaaaauug acacaaaagu uagcgugguc     2160

agaggacaau ucacaaguca auuaagaaga cauuggggca ucgacaaaac ucgcgauaca     2220

uaccaucacc acgccgucga cgcgcuaaua aucgccgcau cuucacagcu aaacuuaugg     2280

aagaaacaga aaaacaccuu aguuucuuac agugaagauc aauuguuaga uauugaaacu     2340

ggagaacuua uuagcgauga cgaauacaaa gaaucaguuu uuaaagcucc uuaucaacau     2400

uucguagaca cauuaaaaag uaaagaauuu gaggacagua uccuauucag cuaccaaguc     2460

gauagcaaau uuaauaggaa aauaagugac gcuacgauau acgcuacuag acaagcaaaa     2520

guaggaaaag acaagaaaga cgagacguau guguugggua aaaucaaaga uauuuacuca     2580

caagcgggau acgaugcauu uauuaaaaua uaccaaaaag auaaauccaa auuucuaaug     2640

uaccgacaug auccccaaac auucgaaaaa guaauagaac cuauauuaga aaauuaccca     2700

aacaaagaaa ugaaugaaaa agggaaagaa gucccaugua aucccuuccu uaaauacaaa     2760

gaagaacacg gggacuauau ccgaaaauau agcaagaaag gaaacggacc agaaaucaaa     2820

ucccuaaaau acuaugauag uaaauuggga aaccacauag acaucacacc caaaaauagu     2880

aauaauaaag uagucuugca aaguguaucu ccauggagag cagacgucua cuuuaacaaa     2940

acaacuggua aauacgaaau cuuaggacuc aaauacgcag accuccaauu ugaaaaagga     3000

acgggaacau auaaaauaag ucaagaaaaa uauaacgaua ucaaaaagaa agaaggagua     3060

gacagcgauu cagaguucaa auuuacccug uacaaaaacg accuuuuauu aauaaaagac     3120

acagaaacga aagaacaaca acuauucaga uuucucucuc gcucaaugcc caauaagaaa     3180

cauuauguag aacuaaaacc auacuccaaa gacaaauucg agaaaaacga aacacucaua     3240

gaaauccugg gacccacaga caaaaguggg aggugcauca aaggacuugc caaauccaac     3300

uuaucaaucu ucaaaguaag aacggauauc uuaggcaacc aacauaucau uaaaaacgaa     3360

ggugacaaac caaaacuuga cuuuuga                                         3387


<210>  5133
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5133
augacaaaaa uccucggucu agauuuaggu acaaacucca ucgggugggc cgucguugac       60

gaaaaaaaag aaaaaauauu agggaugggc ucaagaauau ucccggaagg aguuguaaac      120

auaggagaag gagaaggccg ugaaacuagc aaaaacgcaa guagaacaga cgcgagaggc      180

acacgcagac aauucuuuag gcgaaggcuu cguaaacgau acuuauuacg ugaacuagca      240

aaaaaaggac uauguccaau caacuacacu cuaguuaaag aguggaacga agcucaaaua      300

uucaaaaaca aacaauuaca agaaugguuu aaacuuaauc cauacgaauu acguucaaaa      360

gcaauacaag aaaaaauauc auuggaagaa uuaggaagga ucuucuauca uuucauacaa      420

agaagagguu uccagucuaa cagccgaagc gcaggcguag acaacauaga aaaaucaguc      480

aucuuuaaag gugacacgaa aacgggcaaa auaggaauau ccgaaacauu agaaaguaua      540

aaaaacacaa aaacacuagg auccuacuua cacacuauau auccaaaaca aaacaaacca      600

uauaguggcc acaaagaacg cauaagaaac agguacacaa cacgccaaau guacaucuac      660

gaauuugaaa cuauauggga auaucaaaaa caguucuaca gugaacuauc aaacgaacua      720

aaaacaguac uagguggucg caaaaaagac ggauacaaag aggauggaau acucuuccau      780

caaaggcccc uaagaucaca aaaacaucug guaggauacu gcaguuuuga gccaaaaaaa      840

acaaaauguc aaauaaauac aauaccauuc gaacuauuuc gcauauauca augggugaau      900

acagucgauu gcaacgaaaa aguaaccuca gaagaucgag gaaaaauggu agaacuucua      960

uuaagcaagg aaaaaguauc auucaaagaa auaagaaaag uaauaaauaa aucagaugga     1020

uacuaucaau uuaauuacaa aaaugacgac aaaauaguag ggucauacac aauuucaaau     1080

cuaaguaaua aaaaguuuuu uggaaaaaag ugguuugauu ucagcgacaa agaacaagaa     1140

gauauauggc acguacuuuc uuucuucgac gauagagaua aacucaaaca auacgcaauu     1200

aaauauuggg guuuuaauga agaaaaagcu guaaaaauaa gcaaauuuaa cuuaaaagag     1260

ggauacucaa acuuaucacg caaggcaauc cauaacaucc ucccauuccu aaaacuagga     1320

uucauguaug acgucgcagu cggacucgga ggcguaaaga augcccucgc cgacaaaugg     1380

auagaaaacg aacaguuuau uuuagacaac guaccuagug uaguccguag caauuucaag     1440

gguggcuaca uagaaccauu aaaacaaaug cugaaauacg aauuugacgu cucagauaaa     1500

caacuacuaa aacuauacca ccacagcagu gcaauugaaa ccacggaauu guuaaaaaaa     1560

uuaccuauca acuccgaagc cgacaaagaa auucaaagca ucaaaaaucc aguaguaaua     1620

accgcauuau ucgaaauaag aaaauugguu aaugagauaa uagauacgua cggaaaauuu     1680

gaugaaauca aagucgaacu cgcaagagau uuaaaagcuu caaaaagcaa acgcuacgac     1740

acaagaaggg aacaauuaag acuagaaaaa gaaaaugacc ggauuaaaau agaacucgaa     1800

aacuuaggac aaagaauaaa ucacauaaca auccuaaaau acaaacuaug ggaagaaugc     1860

gacaaaagau guccauuuac aggaaaaaau aucgaaguua acgaacuguu cagcggagaa     1920

guacaaauag aacacauauu ccccuggucg agaucuuuaa augauaguuu uaugaauaaa     1980

acacucuguu ucgccgauga aaaccgagca aaggggaaua aaacccccua ugaauuuuau     2040

agugaacaag gagaacaaaa augggaagaa auaaaaauuc aagcccuaag uugcuucaaa     2100

aauaaaccga acuaucccaa cgccuaucaa aaauuuaaac aguucguaaa aaaaaaacau     2160

gaugaagacu uuauaagcag acaacucaac gacacucgau auauaagcaa agaagcaaaa     2220

aauuaucucu cgaaaauaug caacaacgua caaguagcac ccggacaaau gacagcaacu     2280

cuaagacaua aauggggacu aaacacaauu uuaaacaaag augaugacac uaaaacaaga     2340

gacgaccaua gacaccacgc aauugacgca cuuguuaugg cgugcaguac aagaucucau     2400

cuacaggaac ucaguaaacg uaauagguau caaaaagacu acgaauucaa agauuucccc     2460

augccuuggc aauucuuuag acaagacgca gagaaaucag uagaacaaau auugguaucg     2520

cauaaaaaac uuaauacaau cuuaacggua cgaacacaca aaacgaaaaa aaacggagua     2580

gucuacaaaa auauaggagu agccgcccga ggucaacuac auaaagaaac gguauucgga     2640

aaaagaacag cuccagaugg uauagaagca uaucacguac gcaaaccaau agaaucgcua     2700

uccacacaca aacauauaaa caaaguuguc gacgaaacaa uuagauuacu aauucuuaaa     2760

caaguuaaca aacugggcgg auucguaaaa gguaaaaucc cugagggaac cuuuuauaac     2820

acagaugaaa acggaaacaa acaaccacaa aucuuccuac cuaacaaaaa uggaaauccc     2880

guaccaauaa aaaaaauacg uuuacgagaa aacauaggag gagcagaaca acuaaaaagc     2940

acagucaacc aacacguaaa uccuagaagu aaccaccacg uacuaaucua caaagauuuu     3000

gacgauaacc uuaaagaaaa gguggucaca uucuggacag cggucgaacg aaagaaacaa     3060

aaacaagcag uauuccaauu accugaaaau gggaaagaaa uuguuacuac uuuacaaaua     3120

aaugacaugu uuuuauuagg cuugaaagac gaugaaauaa acugggaaca cccaaaauuc     3180

accuuacuca aagaacauuu auaucgagua caaaaauuca cgucaggcga cuacuacuuc     3240

cgcaaacaca uagagagcaa ucuaaaugga gagcuaggua aagcuuacca auacaucaaa     3300

ggauucggug aaggaaaaac cggauggaaa acauuuaauc caaucaaaau aaaaguuuca     3360

guuucaggaa aaauagaaaa aguauga                                         3387


<210>  5134
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5134
augaauggac ugguauuagg acuagauauc ggaauagcau ccgucggugc aggaauacua       60

aagaaagaca caggcgaaau cauucacgca aauaguagau uauucccagc cgcaaccgca      120

gauaauaacg ucuuaaggag aucaucaagg caagcucgac guagcacucg acgaaaaaga      180

cauagagcug ucagacuaca cgaucuauuu gaagauuacg gguuacugac cgauuucagc      240

aaagugucaa uaaaucugaa uccauacaaa cuacgaguag agggaaugga cauccaauua      300

acuaccgaag aauuauuuau agcacuuaaa aauauuauaa aaagaagagg aauaaguuau      360

cucgacgaug ccagugagga cgggguaaca guuucaucag acuacggaaa agcaguagaa      420

gaaaacagaa aauuauuaag cgaacagaca ccaggucaaa uacaacuuga aagauucgag      480

aaauauggac aaguaagagg cgacuucaca guagaagaaa acggcgaaaa gagacgacua      540

auuaacguau uuaguacauc aacauauaga aaagaagcag aacgaauauu acgaaaacaa      600

caagaguuca acaauaaaau aacagacgau uuuauagacg acuacuuaac aaucuuaacc      660

ggaaaacgaa aauauuauca cggcccagga aacaagaaau cuagaacaga uuacgggaga      720

uauacaacaa agaaagauag cgaagguaaa uacauaaccc ucgacaacau cuucggagua      780

cuaauuggaa aaugcacauu uuauccagau gaauauaggg cuucaaaagc cucauacaca      840

gcucaggagu ucaaccuccu aaaugaccug aauaaccuaa caguaccaac ugaaacaaag      900

aaacucucag aaaaacagaa gaaaacaaua gucgaauacg caaaaacggc aaaaacacua      960

ggggcaucca cacuacuaaa auacauagca aaaaugguag acgcaagcgu agaccaaauc     1020

cauggauauc gagucgacgu aaacaagaaa ccggaaaugc acaccuuuga cguauacagg     1080

aaaaugcaaa gccuagaaac cuucaacgua aaugaaaugc caagagaagu acuagacgaa     1140

cuagcacaua uauuaacucu gaacacagaa agagaaggca uugaagaagc aaucaacaca     1200

aaauuaaaag ggaccuuuaa ccaagaucaa guauuagaau uaguccaauu ccgcaaaaac     1260

aauaguagcu uauuuagcaa aggauggcac aacuucucau uaaaacuaau gauggaacua     1320

auuccagaau uauacgcgac uucugaagaa caaaugacaa uauuaacaag acuagguaaa     1380

caaaaaucca aagagauuag caaaagaacu aaauacauug augaaaaaga aauuacagaa     1440

gaaauuuaua auccaguagu cgcgaaaagc guacgacaag cuauaaaaau cauuaacgaa     1500

gcaacaaaac aauauggcau auuugaucau auaguuauug aaauggcaag acuaacaaau     1560

gaagaggacg caaagaaaga cuauauuaaa agacaaaaag caaaucuuga ugagaaaaau     1620

gccgcaaugg aaaaagcagc uuuucaguac aacgguaaga aagaacugcc agauuccaua     1680

uuccauggac auaaagaacu cgcuacgaaa aucagacuau ggcaccagca aggcgaaaaa     1740

ugccuauaca cgggcaaaaa cauacccaua agugacuuaa uacaaaacca auauaaauac     1800

gaaauugacc acauacuacc auuaucacuc agcuuugacg acucuuuaag uaacaaagua     1860

uuaguccuag ccacugccaa ccaagaaaaa ggacagagaa caccuuuuca agcacucgau     1920

agaauggacg acgcuuggag cuauagagaa uuuaaaagcu acgucaaaga uucaaaauua     1980

cuaggcaaua aaaagaaaga cuaucuccuu acagaagaag acauaucaaa aaucgaaguu     2040

aaacaaaaau uuaucgaaag aaauuuaguu gacacuagau auucaucaag aguaguauua     2100

aacgcauuac aagauuucua caaaaaucau cauuucgaua caaccaucuc cguaguucga     2160

ggacaauuua caagucagcu acgacgaaaa uggggacuag aaaaaucaag agaaacuuac     2220

caccaccacg ccguagaugc auuaauuaua gcugcaucau cacaauuacg acuuuggaag     2280

aaacaaaaca auccacuaau auucuacaaa gaaggacaau ucgucgauuc agaaacaggg     2340

gagauacucu cacuaaauga ugaugaauau aaagaauugg uauucaaagc uccauacgac     2400

cauuuuguag auacccuaag aucgaaaaca uucgaggauu caauccuauu uucauaccag     2460

guugacagua aauacaauag aaaaauaucc gacgcaacaa uauacgcuac caggaaagca     2520

aaauuagaua aagacaaaag caaagaaaca uacguacuuu caaaaauaaa agauaucuac     2580

ucacaagcug guuacgacgc auucauaaaa auauauaaua aagacaaauc aaaauuucua     2640

auguaucaca aagauucaca aacauuugaa aaaguaaucg aagaaauucu acggacuuac     2700

cccaguaaag aguucaacga uaaaaacaaa gaaguaccau guaaucccuu cgaaaaauau     2760

agacaagaaa acggcccuau aagaaaauac ucaaagaaag gaaauggacc cgaaaucaaa     2820

agccuaaaau auuaugacaa aaauauucca gaaucauuca uaaauaucac accaaguaau     2880

ucuaaaaaua aagugauauu gaggcaauua aacccgugga gaacagacgu cuacuucaac     2940

caucaaaccg aaaaauacga auugcuagga cucaaguacg ccgaucuuca auuugaaaag     3000

aaaacaggga cauauucgau aacuauagac aaauacaacg aaauaaagga aaaagaaggg     3060

guaggagaac aaagugaauu uaaguuuacu uuauauaaaa augaucuucu acuuauuaaa     3120

gacacaaaga acaacgaaaa acaaaucuuu cgauuucuaa gucgaaccau gccuaacgua     3180

aaacauuacg uagaacuuaa acccuacaac aaagccaaau uugacggaaa ucaaccacuu     3240

aucacuauau uuggaaaugu ugcuaaaggu ggacaauguc uaaaaggacu aaacaagccc     3300

aacauuagua uauauaaaau uaaaacugau guauuaggua auaaauauuu caucaagaaa     3360

gaaggggaca aaccaaaacu caaauucuga                                      3390


<210>  5135
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5135
auggcggaca ggauaucacu aggauuagac auagggguag cuaguguggg auuuucagua       60

cuggacauag auaaaggcaa aguuaucgag uuagggagca gauuauuuaa cgccacuguu      120

gcagaaggaa aucaagauag aagaagcaug agaggagcaa gaagacuacu caacagaaac      180

aaacagagaa gacaagauac aggaaaauua uuccaaaaau uuggcuuauu agacaauuuc      240

aacaaagaca auuucuacga uaccuucaau caaaauugga auccuuauga auuaagaguc      300

aaaggccuaa cagaacauuu aacaaaaaag gaacuggcug aaagcuuaua ccagaucgua      360

aaacaccgcg ggauaaguua ugcccugaaa gaugcagacg cugaugaagg aggaacagau      420

uacagugcau cacucaaaau aaacagucaa gaacuagcag aaaaaacacc agcucaaauc      480

caauuacaaa gguuaaauga uuauggaaaa guaaggggac agguagucau aggcgaugau      540

ccagauaauc aaaaaguacu auuaaacgua uucccaacau cagcauauga aaaagaagca      600

aaacagauaa uagcaacaca aaaacaguuc uauccagaaa uacuaaccga ugaauucacg      660

aaagaauacu guaaaauauu aacuagaaaa cgugauuacu ucguagggcc aggcaacgaa      720

aaaucacgca ccgauuaugg aauauacaaa acagauggac gaacauuaga uaacuuguuc      780

gaagaacuua uaggccauga caaaauuuau cccgacgaau uaagagcauc agcagcguca      840

uauaccgcuc aauuauuuaa cgugcuaaau gaccugaaca aucuacggau auuaaguuau      900

gaagauggaa aacuaacuaa agaggacaaa gaaaaaauaa uagcagaaau aaaaucaaac      960

accacuacaa uuaacaugcu aaacgugaua aaaaaagucg cugguuguag caaagaagac     1020

auaaaaggau ucagaguaaa ugaaaaagau aaaccagaaa uuagcucgau ggccguguau     1080

agaaaagucc acaaagaucu gcucaaagua ggaguagaca uauccgauug gccaguugaa     1140

uucauugaca aacuaaguuu uauuuuaaca cuaaacacug aaaacggcga aauaagaaag     1200

caguuaaaua aucgucuagu accagaauuu gauuuucuaa augacgacuu aauccaacua     1260

auuauugaua acaaagacag cuucgaaaua aaaacuaaca auaaauggca uagauucucc     1320

cuaaaaacca ugaacaaguu aaugccagaa augcuggaac gcccagucga acaaaugaca     1380

cuauuaaaug aaaugggacu uguaaaaaaa gacaaaaaaa gauucgaaaa caauaaauau     1440

cuaccauaua aagagauagc aaaagauaua uucaacccag ucgcuucaaa gucaguaaga     1500

gaagcacuaa aaauaguuaa cgcaguacua aaaaaauaug ggcauaucga cuaucuagua     1560

auagaaaugc caagagauaa aaaucuaaaa gaagaacaag acaacauaaa agaauuccaa     1620

aacaaaaaua aaaaagccaa ggaugcagcc uuugaagcau uugucaaaag cguaggcuca     1680

gaacaacgcg ugaaagaggc uuuaucaaaa aacagaaaau ugcaaaugaa aaugcgacug     1740

ugguaccaac aacaggagau ugauccuuac aacggagaaa caauugacgc cacagaccua     1800

auaaacaauc cagacaaguu ugaaauagac cacauaaucc cacaaaguau cagcuuugac     1860

gauagcauua auaauaaaac acuauguuuu gcaucaauga accaaguaaa aggccaaaaa     1920

acuccguacg aguucaugcu ugaaggacau ggucaaggau augacaaauu caaagccacu     1980

guacuagaua auaaaaauuu cgacaggaau aaacgaaaaa auuaucuuuu ugaagaaaac     2040

gucagugaca uagaaacgag aaaaagauuu cuuagcagaa accuagugga uacgagauac     2100

aguucucgug uagucuuaaa cagucuccaa gacuucuucc acgaaaaaag cgcagacaca     2160

aaaguaacag uaauaagagg uaaauucacc ucuaauaugc gcaaacacug gcauauagac     2220

aaaacaaggg aaacauuuca ccaucaugcg auagacgcca gcaucauagc agccacaccc     2280

uuccuuagaa uguggaagaa agguggaacu auauuuccgg uuaagguagg agaagaaagc     2340

auagacauag aaacaggaga aauacuagau gauaaaaaau uugauaaagu uauguaugaa     2400

gaaccauaca acggauuugu aucagaaauc augaaugccg acgaccggau aaaauuuagu     2460

caccaaguag acaaaaaaau gaaccgaaaa gucagugaug cuacuauaua uagcacccga     2520

acaggaaaac uggcaaaaga uaaaaaagac gcagaauaca uaguagcaaa gguuaaagau     2580

auauauagcg uagacggguu uaaaaaauuc aaaaaagucu augacaaaga uaaaaguaaa     2640

uuucuccuau acaaauauga cccgagaaca uucuccgaac uagaaaggau uauaucagau     2700

ugcccugaua aaguagagaa aguacaaaca aauggaaaag ugaaagccgu agacguaagu     2760

ccauucgaaa uguauagaag guaccacggc augauaaaaa aauauaguaa aaaaggaaau     2820

ggaccugcca uuaaacaacu aaaauacuua gacaagaaau uagguucgca uauagacaua     2880

acacccgcca augcaaaugg caaacacgua auauugcaau cacuaaaacc cuggcguacu     2940

gauguauacu uaaaucauga aaccggagaa uacgaaauaa ugggaauuaa auacuccgau     3000

uuaaaguuca acaaaaaugu uggauauggu auaaagaaag auaaauaucu agaaauaaaa     3060

aaaaacgaag aaguaaguga aaaaagcgaa uucauguucu cacuuuaccg aaaagaucgc     3120

guucgaaucc aaaacaugaa aaccggagaa aguguagaau uacuguucug gucaagaaac     3180

uuuucgaaca aaaaauaugc cgaacuaaag ccaauauuua aauuugucac agaagacgac     3240

gauuggccag uuuacggaca ugcgaaaacu caaauauuaa aacgacuggu acccaaagac     3300

uguaaaauau ggaagguaaa caccacaaua cuaggagacc ccuacuauuu agaaaaagaa     3360

uccgacaacc caaaagauau auuagacuga                                      3390


<210>  5136
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5136
augacaaacg gcaaaauccu uggucucgau aucggaaucg cuagcguggg aguggguauc       60

aucgaggcaa aaacuggaaa aguuguacau gcaaauagcc gccuauucag cgcagcaaac      120

gccgaaaaca acgccgaaag aagaggauuc cgagguucaa ggagacucaa uagacgcaag      180

aaacacagag uaaaaagagu cagagaucua uucgaaaaau auggaauagu aacagauuuc      240

cguaaccuaa aucuaaaucc uuaugaauua cgaguuaaag gauuaacgga acaacuaaca      300

aacgaagaau uguucgcagc uuugcgaacu auaaguaaaa ggcgaggaau aaguuaucua      360

gaugaugcag aagacgacag cacaggaucg acagauuaug ccaaaagcau agaugaaaac      420

cgacgguugu uaaaaacgaa aacaccagga caaauccaac uugaaagacu agaaaaauau      480

ggacaacucc guggaaacuu caccguauac gaugaaaacg gugaagcaca uagauuaaua      540

aacguguuuu caacaaguga cuacgaaaaa gaggcgcgaa aaauauuaga aacucaagca      600

gacuacaaua agaaaaucac ugcagaauuu auagacgauu auguugaaau acuuacucaa      660

aaacguaaau auuaucaugg accgggcaac gaaaaaucaa gaacagauua cggacgguuu      720

agaacagaug guacaacccu cgagaauaua uuuggaauau uaauaggaaa auguaguuuc      780

uacccugaug aauaccgggc aucgaaagca aguuauacug cgcaagaaua caauuuucua      840

aaugaucuaa auaacuuaaa aguaccaacg gaaacaggaa aacucagcac agaacaaaaa      900

gaagcguuag uagaauuugc aaaaucaaca gcaacccuug gaccagcaaa acuauuaaaa      960

gaaaucgcaa aaauuuuaga cuguaaagua gacgagauaa aaggauaucg agaagacgau     1020

aaaggaaaac cagauuuaca uacuuuugaa ccauauagaa aacuaaaauu uaaccuagau     1080

aguguaaaca uagacgaucu gucucgagaa guacuagaca aauuggcaga cauauuaaca     1140

cuaaacacag aaagggaagg aaucgaagau gcuaucaaaa gaaauuuacc aaaccaauuc     1200

acagaagaac agauuucaga aauaauaaau guaagaaaau cgcagucuac agccuucaac     1260

aaaggguggc acagcuuuuc cgccaaacuc augaacgaac uaauuccgga auuauaugcc     1320

accucagaug aacaaaugac aauauuaacu agauuagaga aauucaaagu caaaaagaaa     1380

aguagcaaaa auauuaaaac uauagacgaa aaagaaauca cagacgaaau auacaauccc     1440

guagucgcaa aaucaguccg acaaacgaua aaaauuauaa acgcugcagu aaagaaauac     1500

ggagacuuug auaaaauagu aauagaaaug ccgcgugaua aaaaugcaga cgacgagaag     1560

aaauucaucg acaaaagaaa caaagaaaac aagaaagaaa aagaugacgc auuaaaaagg     1620

gcggcauauc uuuauaaugg uacagacaaa cuuccugacg aaguauuuca cgaaaacaaa     1680

caacuggaga caaaaauacg acuuugguau caacaaggag aaagaugucu auauuccgga     1740

aaacccauac cuauucaaga guuaguacac aacagcaaca acuucgagau ugaccacauc     1800

cuaccauuau cacucucauu cgacgacuca cuagcaaaca aagucuuagu auauagcugg     1860

acaaaccaag agaaaggaca aagaacaccu uaucagguaa uagacaguau ggacgccgcu     1920

ugguccuuca gagaaaugaa agauuaugua cucaaacaaa aaggauuagg caaaaagaaa     1980

cgagauuauu uauuaacaac ugaaaauaua gauaaaauag aggugaaaaa gaaauuuauu     2040

gaacgaaauc uuguagauac aagauacgcc aguagaguug uacuaaauag cuuacaaucc     2100

gcucucagag agcuggggaa agauacaaaa gugagcguag uaagagguca auuuacaucu     2160

caauugagga ggaaauggaa aauagacaaa ucaagagaaa cauaucacca ucaugcuguu     2220

gacgcauuaa ucauagccgc aucuagucag cuuaaacuau gggaaaaaca agacaauccc     2280

auguucguag acuauggaaa uaaccaagua gucgauaaac aaacuggaga aauccuuagc     2340

gugucagaug augaauauaa agagcuagua uuucaaccuc cauaucaagg cuuuguaaac     2400

acaaucucgu caaaaggauu cgaagacgaa auacuguuca gcuaccaagu agauagcaaa     2460

uacaaucgaa aaguaucaga cgcaacuaua uacaguacac gaaaagcuaa aauaggaaaa     2520

gacaagaaag aagaaacaua cgucuuaggc aaaauaaaag acaucuauag ucaaaacgga     2580

uucgacacuu uuguaaagaa auacaauaaa gauaaaacac aguucuuaau guaccaaaaa     2640

gacccacuca cgugggaaaa cguuauugaa guaauuuuaa gagacuaucc aacgacaaag     2700

aaaucugaag auggcaaaaa ugacguaaaa ugcaauccau ucgaagaaua cagacgagaa     2760

aacggauuga uaugcaaaua uucuaagaaa ggaaaaggca caccaauuaa aucauuaaaa     2820

uauuaugaua agaaauuagg aaauuguaua gacauuacac cucaagaaag caaaaaccgu     2880

gugguauuaa gacaaauaag cccauggaga gcggacauau acuuuaaccc agaaacccua     2940

aaauaugaac ucaugggucu aaaauacuca gaccuaucau ucgaaaaagg cacugguaaa     3000

uaccauauau cgcaagagaa auaugacgcg auaaaagaaa aagaaggcgu aggaaaagac     3060

agugaauuua aauuuacauu auaccguaac gacuuaauac ucauaaaaga cacacugaac     3120

aacugugaac gaaugcugcg uuuuggaagc aaaaacgaca caaguaaaca uuacguggag     3180

cugaaaccau uagaaaaagg aaccuucgau agugaggaag aaauuuuacc aguacuagga     3240

aaaguggcca aaucagggca auucauaaaa ggacuaaaca aaccaaauau uucuauauau     3300

aaaguuagaa cagauguauu aggaaauaaa uacuuuguca agaaagaagg cgauaaacca     3360

aaauuagauu uuaaaaauaa caagaaauga                                      3390


<210>  5137
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5137
augucgaacg gaaaaauauu aggacucgac aucgguguag ccaguguagg cguugguaua       60

auagauucaa aaacaggaaa uguaauacac gcaaacagua ggcuauuuuc cgccgcaaac      120

gcugaaaaca augccgaaag aagaggauuc cgcggagcuc gaagacuaac uagaagaaag      180

aaacauagag ucaaacgcgu aagagaccua uuugaaaaau augauauaag cacugauuuu      240

agaaaccuca aucuaaaccc auacgaauua agaguuaaag gauugacaga acaauuaacg      300

aaugaagaac uauucgcagc gcuaagaacu auagccaaac ggcgagguau auccuaucua      360

gaugaugcag aagaugauuc gacaggaagc agcgacuaug caaaauccau agaugaaaac      420

aggcgcuuau uaaaaacuaa aaccccggga caaauccaau uagaacgacu agaaaaauau      480

ggucaacuaa ggggcaacuu uacaguguac gacgaaaacg gugaagcaca ccgauuaaua      540

aaugucuucu cuacaucuga cuacaaaaac gaagcuagaa agauccugga aacucaaucc      600

aauuauaaua aacaaauaac agaugaauuc auagaagauu acauagaaau auuaacacaa      660

aagaggaaau auuaucaugg cccaggaaac gagaaaagua gaacagauua cggacgcuuu      720

agaacagaug gaacaacgcu ugaaaacauu uucggcauau uaauaggaaa gugcucuuuc      780

uauccggagg aauaucgagc aucaaaagca ucauacacag cccaagaauu caauuucuua      840

aacgaccuaa auaacuuaaa aguaccaacg gaaaccggga aacuaucaac agaacagaaa      900

gaguacuuag ucgauuucgc caaaaaaagu aaagcucucg gugcuucaaa acuauuaaaa      960

gaaauagcaa agauagugga cuguucggua gacgacauaa aaggauaccg gguugacaau     1020

aaagacaaac ccgacuugca uacguuugaa ccauacagaa aauuaaaauu caauuuaagc     1080

ucaaucgaca uagacgaauu aucaagagaa acacuugaua aacuagcgga uauauuaacu     1140

uuaaacacag aaagagaagg cauugaagac gccaucaaaa gaaacuuacc aucucaauuu     1200

acagaggaac aaauauccga aauaguacaa auaaggaaaa accaauccuc agcuuucaac     1260

aaaggauggc acaguuuuuc agcaaagcuc augaaugaau uaauaccaga gcuauaugua     1320

acuucggaag aacaaaugac cauccuaacu cgacuggaaa aauuuaaggu caauaaaaaa     1380

agcucuaaaa acacaaaaac aauagacgaa aaagaaauaa cagaugaaau auacaaccca     1440

gugguagcaa aaucagucag acaaacaaua aaaauaauaa augcagccgu uaaaaaauac     1500

ggugauuucg acaaaauagu gauagagaug ccaagggaua aaaacgccga agacgaaaaa     1560

aaauuuauug acaaaaaaca aaaagaaaau aaaaaagaaa aagacgauuc auuaaaaaga     1620

gcagcguuuu uauacaacgg uaccgacaau uuaccagacg guguauucca cggcaacaaa     1680

gaacuuaaaa ccaaaauacg auuaugguac caacagggcg aaaggugucu uuacaguggg     1740

aaguuaauaa guauccauga ucuaguucau aauagcaaca aauucgaaau cgaccacaua     1800

uuaccacuaa gucuaaguuu cgacgacagu cuagccaaua aagucuuagu cuaugccugg     1860

acaaaucaag aaaaaggaca aaaaacaccc uaccaaguca uagacucaau ggacacugca     1920

uggucauuuc gagaaaugaa agacuacgug uugaaacaaa aaggauuagg caaaaaaaag     1980

ugugaauacc uauuaacaac ugagaauaua gacaaaauag aaguuaaaaa aaaauucaua     2040

gagagaaacc uaguugauac uagauacgca agucgagugg uacuaaauuc acugcaaacc     2100

gcacuaaaag aacuaggaaa agacacaaaa guaagcgucg uaagagguca auucacgagu     2160

cagcuacgaa gaaaauggaa cauagacaaa ucacgagaga cuuaccacca ucacgcaguc     2220

gacgcauuaa ucauagccgc aaguucccaa uuaaaacuau ggcaaaaaca agaaaacccu     2280

auguuugaau cauauggaga aaaucagguc guaaauaaag aaacgggaga aauacuguca     2340

aucagugacg acaaguauaa agaacuggua uuccaaccgc ccuaccaagg auuuguaaau     2400

acuauaagca guaaagguuu ugaagaugaa auauuauucu cauaccaagu agauagcaag     2460

uucaauagaa aaguaucaga cgccacuaua uacaguacaa gaaaagccaa auugggaaaa     2520

gauaaaaaag augaaacaua cguucuuggc aaaauaaaag auauauauuc acaggauggu     2580

uucgauacau ucauaaaaag auauaaaaaa gauaaaacuc aauuccuaau guaucaaaag     2640

gacccacuaa caugggaaaa uguaauagaa guaauacuaa gagauuaccc aagugaaaaa     2700

cugucugaag acgggaaaaa aacuguaaaa ugcaacccau uugaagaaua uagaagggaa     2760

aacggacuaa ucugcaaaua uucaaaaaaa ggaaauggaa caccaauaaa aucauuaaaa     2820

uauuaugaua aaaaacuagg aaauuguaua gacauaacac cggaaaagag uaaaaaccga     2880

guaguauuaa gacaaauaag cccauggaga gcagacaucu auuucaaccu ugaaacuuua     2940

aaauacgaac uaaugggccu aaaauacucu gauuuaucau ucgaaaaagg aacaggaaaa     3000

uaccacauau cgcaagaaaa auacgaugca auucgagaaa aagaagggau aggcaaaaaa     3060

ucagaauuua aauuuacacu auauagaaac gacuugaucc uuauaaagga cacauuaaac     3120

aauugcgaaa ggaugcuaag auucggauca aaaaacgaca caucaaaaca cuacguagaa     3180

uuaaaaccgu uagaaaaagg gacauucgau uccgaagagg agauucuacc cguucucggu     3240

aaagucgcua aaucaggcca auucauuaag ggauuaaaua aaccaaauau uucaaucuac     3300

aaaguaagaa ccgauguacu aggaaacaaa uucuucauua aaaaagaagg cgacaaaccg     3360

aagcuggauu uuaaaaauaa caauaaauga                                      3390


<210>  5138
<211>  3390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5138
augaguaacg gaaaaauacu aggcuuggac auaggaguag caucaguagg gguaggcaua       60

auagaugcga aaacgggcaa cguaauccau gcuaacaguc gauuauucuc agcagccaac      120

gccgaaaaca augcugaaag aagaggguuc aggggagcaa gacgacuaac aagaagaaaa      180

aaacauagag ucaaacgagu aagagacuua uucgaaaaau augacauuag cacggacuuu      240

agaaacuuaa aucucaaccc auacgaacuc agaguuaaag gauuaacaga gcaauuaaca      300

aacgaagagc uuuucgccgc ccuaagaaca auagcaaaaa gaagaggaau auccuaccua      360

gacgacgcag aagaugacuc gacuggaagu ucagauuaug cuaaaaguau agacgaaaau      420

agaagacugu uaaaaacuaa aacuccuggu caaauccaac ucgaacgacu ugaaaaauau      480

ggacaacucc gagggaacuu cacuguauau gaugaaaacg gcgaagcuca ccgauuaaua      540

aacguauucu ccacaucaga uuacaaaaac gaagcaagaa agaucuuaga aacccaaagu      600

aauuacaaca aacaaauaac cgacgaauuc auagaagauu acaucgaaau acuaacccaa      660

aaacggaaau auuaccaugg accagguaac gaaaaaaguc guacagacua cggaagguuc      720

aggaccgaug guaccacacu agaaaacaua uuuggcauac uaauaggaaa augcucauuc      780

uacccagagg aauaucgcgc cagcaaagcc agcuacaccg cacaagaauu caauuuucug      840

aacgaccuaa acaacuugaa aguaccgacc gaaaccggaa aacuaagcac agaacaaaaa      900

gaguaccuug ucgacuucgc aaaaaaauca aaagcacuug gcgccagcaa acuccuaaaa      960

gaaauugcca aaauagucga uugcucugua gaugacauaa aaggcuauag aguagauaac     1020

aaagauaaac cagaucuaca uaccuucgaa ccauaucgca agcuaaaauu uaaccucuca     1080

aguaucgaua uagaugaauu aucacgagaa acucucgaua aauuagcaga cauuuuaaca     1140

cuaaacacag aaagagaagg cauagaagau acaauaaagc gaaaccuacc uagucaguuc     1200

acagaagaac aaaucuccga aauaguacaa auacgaaaaa aucaaagcuc agcuuucaac     1260

aaaggcuggc acucauucuc agcaaagcua augaacgaac ucauacccga acuauacgua     1320

acaagcgaag agcaaaugac cauacuaacu agauuagaga aauuuaaagu caauaagaaa     1380

agcaguaaaa acaccaaaac uauagaugag aaagaaauaa ccgaugaaau uuauaacccg     1440

guagucgcga aaagcguuag gcagacgauu aaaaucauaa acgccgcagu aaaaaaauau     1500

ggugacuucg auaagauugu aaucgaaaug ccaagagaua aaaaugcaga agacgaaaaa     1560

aaguucauag acaaaaaaga gaaagaaaau aaaaaagaaa aagaugacuc acuuaaacgc     1620

gcagcauucc uuuauaacgg caccgauaac cuaccggacg gcguauuuca cggaaacaag     1680

gaauuaaaaa caaagauacg gcugugguac caacaaggag aaagaugccu guacucaggg     1740

aaacucauua guauacacga ccuaguacau aacuccaaca aauucgaaau agaucauaua     1800

cuaccgcuau cccuaaguuu cgacgauuca cuagcaaaca aaguacuugu auacgcaugg     1860

acaaaccaag aaaagggaca gaaaacuccu uaucaaguaa ucgacagcau ggaugcggca     1920

uggucauucc gagagaugaa agacuacgua cuaaaacaaa aaggcauugg aaaaaaaaaa     1980

agggaauauu uauuaacaac ugaaaacaua gacaaaauag aagugaaaaa aaaauuuaua     2040

gaaagaaacu uaguagacac ucgcuaugcu ucgcguguag uucuaaacuc auuacagaca     2100

gcucucaaag aacuuggaaa agacacgaaa gugucaguug ucaggggaca auuuacauca     2160

caacuacgac gaaaauggaa aauugauaaa agcagagaaa cauaucacca ccaugcagua     2220

gacgcacuga uaauagcagc aucaggacaa cuuaaacucu ggcaaaaaca ugaaaaucuc     2280

auguucgaaa auuacgguga aaaucaggua guaaacaaag aaacugguga gauauuaagc     2340

auaucagacg acgaauacaa agaacugguc uuccagccuc ccuaucaagg cuucgucaau     2400

acaauaucaa gcaagacuuu ugaagacgaa auacuauuca guuaccaagu agacaguaaa     2460

uucaauagaa aaguaaguga ugcaacuaua uauagcacga gaaaagcuaa guuaggaaaa     2520

gauaaaaaag aagaaacuua cguauuagga aaaauaaaag acauauauac acaagacgga     2580

uucgacaccu ucauaaaacg auacaaaaag gacaaaacac aauuucuaau guaucaaaag     2640

gacccccuaa caugggagaa uguaauagaa gugauccuac gcgacuaucc auccgaaaag     2700

uuaucagaag acggaaagaa aacagucaaa uguaaucccu ucgaagaaua uagaagagaa     2760

aacggucuaa ucugcaaaua cucaaaaaaa ggaaauggca caccaauaaa aagccuaaaa     2820

uauuacgaca aaaaacuugg uaauuguaua gauauaacac cagaaaaauc caaaaacaga     2880

guuguacuca gacaaauaag uccauggagg gcagacauau acuucaaucu agaaacauua     2940

aaauacgaac uuaugggacu aaaguacagc gaccucucau ucgaaaaagg aacaggaaaa     3000

uaccacauuu cacaagaaaa auacgaugca auaagagaaa aagaagggau agguaaaaaa     3060

ucagaauuca aauuuacauu auacagaaac gacuuaauuu ugauaaaaga cacucuuaac     3120

aauugugaac gcaugcuacg auuugguagc aaaaaugaca ccaguaaaca uuauguagaa     3180

cuaaaaccuc uagaaaaagg aacauucgau ucagaagaag agauucuucc aguucuagga     3240

aaaguagcaa aaucuggaca auuuauuaaa ggucuaaaua aaccgaacau aagcaucuac     3300

aaaguacgaa cagacguauu ggguaacaaa uuuuucauca aaaaagaagg agacaaacca     3360

aaauuagacu uuaaaaacaa caauaaauga                                      3390


<210>  5139
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5139
augaauaaaa uucuaggacu agacuugggg acaaacucaa uaggaugggc ccuuauagaa       60

aacaauauaa uacucggcau gggaucacga gucuucccag aaggaguugu uaaccugggc      120

gaaggugaag gaagagaaac cucgaaaaac gcaucuagaa ccgaagcacg agggguucga      180

cgacaaauau ucagaagaag acuuaggaaa agauaccuau uaaaagaacu ugcuaaacau      240

aaccuauguc caauaucacc aacauuaauu aaagacugga auggaacaga cauauuuaaa      300

aacgaauuaa uuauacaaug guuccaauua aacccuuacg aacugcgagc uaaagcgauu      360

caagaagaaa uaacauuaau agaacuuggc aggauuuucu aucacaugau acaaagacga      420

ggauuucuuu caaauuccag auccgcgggu gcagacguaa aagaaacguc aacuauauuc      480

aaaggcgacg cuaaaauagg aaaaauaggu auaucagaca cauuaaaauc gauagaacaa      540

aacaaaacac uaggaucauu ucuucacuca auacaaccca aagaaaacga accauuuagu      600

ggagaauuag aacgcaucag aaaccgcuac accacccgac aaauguauau agacgaguuc      660

gaaacaauuu gggaguucca aaaacaauac cauucggaac ugaacucuaa ccucaaaacc      720

auucuaggag gcagaaagaa agacaauuac aaagaagaug gaguucucuu ucaucaaaga      780

ccacuaagau cacaaaaaca ccuaguaggu uuuugcaguu ucgaacccaa gaaaacaaaa      840

ugcaaauuau ccgcaauacc auuugaacua uucaggauau accaaugggu aaacacucuc      900

ucguacgacg uuaauggagu aagacagaaa auuacacaag aagaacguaa gaaaauaaua      960

gacuuauuac uuucgaaaga aaaaccaagu uuuaaagaaa uaagaaaagc aauagguaaa     1020

uuggacggaa gcuaccaauu uaacuacaaa gaugacgaca aaauaguggg aucucauaca     1080

aucaguaauc uauccaauaa gaaauucuuc ggaaauaaau gguuugacuu uucagaaaaa     1140

gaacaagaaa acauauggca caucuuaagu uucuuugaug acagagaaaa acuaaaagaa     1200

uacgcgauua aaaacugguc cuuugacgaa gaaagagcag uuaaaaucag uaaauuuaac     1260

cugaaagacg gguauucuaa uuuauccaga aaagcaaucc uaaacauccu cccuuuucua     1320

caaaaaggau uuauguacga uguugcagua ggacuugcag gagucaaaaa uacuuuauca     1380

gucaacuggg aagaacauga acaauuugug cuugacaaug ucccugagau aguaagaucc     1440

aauuuaaaag gcggauacau ugaaccauua aagaacaugc uuaaaaauga guucaauugu     1500

aacgacaaac aauuagaaaa acuauaccau cauagcucag caauagaaac ggaaaauaua     1560

cuugacagac uuccaguaaa ccuacacgca gaucgugaaa uucagaaaau caaaaacccu     1620

guaguaauaa cugcacucuu cgaaauaaga aaauuaguca augaaguaau agacacauau     1680

ggaaaaccca acgaaaucaa agucgaauua gcuagagauc uaaaagcauc uaaaaguaau     1740

agacuagaag cacguaaaag acaaaaagau cuagaaaaac aaaacgaucg aguaaaauua     1800

gaauuagaga auuuaaacca acgaucaaac cauauaaaca uccuaaaaua caaauuaugg     1860

gaagaaugca accacacuug uccguuuaca ggacaaauaa uaacuauuuc ccaacucuuu     1920

acuggagaag uacaaauaga acacaucuuu ccuuggucaa gaucuuuaaa cgacaguuuc     1980

aacaauaaaa cucuuugcuu cgcagacgaa aauagagcca aaggaaacaa aacaccauac     2040

gaauuauaua gcgaacaagg agaccaaaaa ugggaggcca ucaaacucca agcauuauca     2100

ugcuucaaaa ccaaagcuaa uuacccaaac ugcuauaaua aauuuaaaca uuucgugaaa     2160

aagaaacacg augaagauuu cauauccaga caacugaaug auacucgaua uauuucaaaa     2220

gaagcuaaaa auuaucuuag uaaaauaugu uccaaugugc uaguagcacc aggccaaaug     2280

acggcaacuc uacgacauca uuggggauua aauagcauac uuaacuuaga agacgacacu     2340

aaaacacgug acgaccacag acaucaugca aucgacgccc uaguaauggc augcucaacc     2400

cgaucacauc uacaagaacu aucaaaaaga aaccgauaua auaaaaacua ugauuuaaaa     2460

gauuuuccgg accccuggca aaacuuuaga caggacgcgg aaaacgcagu ugcacaaaua     2520

cuugucaguc auaagaaaag caaaucacuc cuaacaguua gaacccacaa aacuauaaag     2580

aaaggcguag aacauaaaaa cauaggaguu gcugcacggg ggcaacuaca caaagaauca     2640

guuuauggac ugcgaaaagc acccaacagc aauaaagccu uccauauucg aaaaccauua     2700

gagucuuuga ccacagaaaa acaacuugag aaaauugugg acgauucaau aagacuacuu     2760

auauuaaaaa gaauaaauau aauuggaggg uuuauuaaag ggaaaauacc aaaagaaaca     2820

uucuucguag uagaugaaaa uggaauaaaa caaccucaaa uauuucuccc aaacagaaau     2880

ggaaacccag uaccgauuaa gaaaguaaga auaagggaaa acauuggugg agcagaaaaa     2940

cuaaaagaga auacaaacca auacguaaau ccacgaucaa accaccacgu caucguuuau     3000

gaagauucga auggaggccu aaaagacgau guaguaacuu ucuggacugc aguagaaaga     3060

aaaagacaag gacaagaugu cuucaaacua ccgaacgacg gagagaaaau cgugucaacu     3120

cuacagauua augauauguu ccuacuagga cuaaacgacg acgacaucau aacauuaaca     3180

aguaaagaac uaaagaaaca ucuauuuaaa guccaaacac uaaguucauc auucuacgag     3240

uuccguuuaa guacagauuc aacacaaaac aaaaauacaa acagcacagu guucagaagg     3300

auacagagcu ucggaauggg uaaaacagga uggaaagcau ucaauccaau aaaaguuaaa     3360

uuaaacucaa uuggcgaagu uaaaauaaua uga                                  3393


<210>  5140
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5140
augacgaaug gcaaaauauu aggacuagac auaggcauag ccaguguugg uguaggaaua       60

auagaagcaa aaacuggaaa aguaguacau gcaaacaguc gauuauuuag cgcagccaac      120

gcagaaaaca augccgaaag acgaggauuu cgcgguucac ggcggcuuaa ccgacgaaag      180

aaacacagag uuaaaagagu gagagauuua uucgaaaaau acggaauagu aaccgauuuu      240

cgaaaccuga aucucaaucc auacgaauua agaguaaaag gauuaacaga acaacuaacg      300

aaugaagaac uauuugcagc uuuacgcaca auaucaaaaa gaagaggcau aucauaucuc      360

gacgaugcgg aagaugacuc aacaggaagu acugacuacg cuaaaagcau agacgagaac      420

agacgauuac uuaaaaccaa aacuccaggg caaauucaac uagaaagacu agaaaaauac      480

ggacaacuga gaggaaacuu uacuguauac gaugaaaaug gcgaagcuca cagacuaaua      540

aauguauuua guacaucaga uuaugaaaaa gaagcccgaa aaauucuaga aacccaagcc      600

gacuacaaua agaaaaucac agcagaguuc aucgacgacu acguagaaau acuaacacaa      660

aaacgcaaau acuaucaugg uccuggaaau gaaaaaucac guaccgauua cgguagauuc      720

agaacagaug gaacaacgcu ugagaacauc uucgggauau uaaucggaaa augcucauuu      780

uauccagaug aauacagagc uucgaaagca uccuauacag cacaggaaua uaacuuuuua      840

aacgacuuaa acaaccuaaa aguaccaacc gaaaccggua aacucucaac cgagcaaaaa      900

gaagcccuag ucgaguucgc aaaaaguaca gcuacauuag gaccugcuaa acuauugaaa      960

gaaauagcua aaaucuuaga uugcaaagug gaugagauaa aaggcuaucg agaagaugac     1020

aaaggcaaac cagauuuaca uacauucgaa cccuacagaa aacucaaauu uaaucuagau     1080

ucaguaaaua uagaugauuu aaguagagaa gugcucgaca aacuagccga cauacuaacc     1140

cugaacaccg aaagagaagg cauagaagac gcgauaaaaa gaaauuuacc aaaccaguuu     1200

acagaagaac aaauaaguga aaucauaaau gucagaaaaa gccaauccac agcguuuaau     1260

aaaggcuggc acucauucuc agccaaauua augaaugaau uaauaccaga acuauacgcc     1320

acaucagacg aacagaugac gauucugaca agguuagaaa aauucaaagu uaaaaagaaa     1380

ucaucuaaaa auauaaaaac cauagaugaa aaagagauaa cagacgagau cuauaaucca     1440

guagucgcca aaucaguaag acaaacaauc aaaauaauua acgccgcagu gaagaaauac     1500

ggugacuuug acaaaaucgu aauagaaaug ccgagggaca aaaacgccga cgacgaaaag     1560

aaauuuauag auaaaaggaa uaaagaaaac aagaaagaaa aagaugaugc ccugaaacgu     1620

gcagcuuacc uuuauaacgg gacagauaaa uuaccggacg aagucuuuca cggaaacaaa     1680

cagcuugaga caaaaaucag gcuuugguau caacaaggag agcgcugucu auacucagga     1740

aaaccaauac caauacagga gcuaguacau aauucaaaua auuucgaaau agaucauaua     1800

cuaccauuaa gccuaaguuu ugacgacucu cuugcaaaca aaguucuagu auauucaugg     1860

acaaaccaag aaaaaggaca aaggacucca uaucaaguua uagacagcau ggacgcagcu     1920

uggucauuca gagaaaugaa agacuauguc cuaaaacaaa aaggguuagg uaaaaagaaa     1980

agagacuacc uuuuaaccac cgaaaacaua gauaaaauag aaguuaaaaa gaaauucauu     2040

gaacgaaauu uaguagauac acgauaugca uccagagucg uacuaaacuc acuacaaagc     2100

gcccuaagag aacuaggaaa agacacaaaa guaagcguag uaagaggcca auuuacauca     2160

caacuacgca gaaaauggaa aauagauaaa ucaagagaaa cauaucacca ucacgcaguc     2220

gaugcguuaa uaaucgcagc gagcucgcaa cugaaacuau gggaaaaaca agauaaucca     2280

auguuuguag auuacggaaa uaaucaagua guagacaaac aaaccggcga aauacuaucc     2340

guuucagaca acgaauacaa agaauuagua uuucaacccc cauaucaagg auucgucaau     2400

acaauuuccu caaaaggauu ugaagaugaa auacuauuuu cauaccaagu ugacaguaaa     2460

uacaauagaa aaguuaguga cgcaaccaua uauucaacaa gaaaagcaaa aauaggaaaa     2520

gacaaaaagg aagaaaccua cgucuuaggu aagauaaaag auauauacuc acaaaacggu     2580

uucgacacgu uuguaaagaa auauaauaaa gauaaaacuc aauuccugau guaccaaaaa     2640

gauccauuaa caugggaaaa uguuauagaa guaauauugg gagauuaccc uacgacaaag     2700

aaaagcgaag acgggaaaaa cgauguaaaa ugcaacccau ucgaagaaua ucgaagagaa     2760

aacggacuaa uaugcaaaua cagcaagaaa ggaaaaggaa ccccaauaaa aucuuuaaaa     2820

uacuaugaua agaaacuagg aaacuguauu gacauaaccc cagaagaguc acgaaacaaa     2880

guaauacuac aaagcauuaa uccauggaga gcagacguau acuucaaccc ugaaacacua     2940

aaauacgaac ucaugggucu aaaauauuca gaucuaaguu uugagaaagg aacaggaaac     3000

uaccauauuu cacaagaaaa auaugaugca auuaaagaaa aagaaggaau uggaaagaaa     3060

ucagaguuca aauucacuuu auaucgcaau gauuuaauau uaaucaaaga cauagcauca     3120

ggcgaacaag aaauauauag auuccuaagu cgcacaaugc cuaacguaaa ccacuacgua     3180

gaacucaaac cauaugacaa agaaaaauuc gacaacgucc aggagcuagu cgaagcacua     3240

ggcgaagcag acaaaguggg gcgcuguaua aaaggauuga auaaaccaaa cauaagcaua     3300

uauaaaguaa ggacagacgu acucggaaau aaauacuucg uaaagaaaga aggcgauaaa     3360

ccgaaacuag acuuuaaaaa caacaagaaa uga                                  3393


<210>  5141
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5141
augaccaacg gcaaaauauu aggccuagac auuggaauag caucaguagg aguaggaaua       60

auagaggcaa aaacaggaaa agugguucac gcaaacucaa gacucuucag cgcagccaac      120

gccgaaaaca acgcagagag acgcggcuuc cgagggaguc gaagacuaaa caggcgaaaa      180

aaacaccgag uaaaacgugu aagagaccua uucgaaaagu augaaauagu gacagauuuc      240

aggaacuuaa accuaaaucc cuaugagcuc cggguaaaag gacugacgga acaauuaaca      300

aacgaagaau uauuugccgc ucuacgaacg auaucuaaac gaagaggaau aucauaucug      360

gaugacgccg aagaugacuc gacagggagc acagauuacg cuaaaagcau agacgaaaac      420

agaagauugc ucaaaacaaa gacccccgga caaauacaau uagagagauu agaaaaauac      480

ggacaacuac gaggcaacuu caccguauac gacgaaaacg gcgaggcaca uagacuaaua      540

aauguauucu caacaagcga cuacgaaaaa gaagcacgua aaauccugga aacacaggcu      600

gacuacaaua aaaaaauaac agcagaauuc auagacgacu acgucgaaau auuaacacaa      660

aagagaaaau auuaccacgg accagguaac gaaaaaagca gaacagacua uggaagauuc      720

cggacagaug gaacaacacu agaaaauauu uucgguaucc uaauaggaaa auguagcuuu      780

uauccagaug aauacagggc cucaaaagca agcuacacgg cucaagaaua uaacuuucua      840

aacgacuuaa auaaccuuaa aguaccaaca gaaacuggaa aauuaucuac agaacaaaaa      900

gaagcccuug uagaauucgc aaaaagcacg gcaacgcuug gaccagcaaa auuacuaaaa      960

gaaauagcaa aaauacuaga uuguaaggua gaugaaauaa aaggauacag agaggacgau     1020

aaaggaaaac cagaccuaca cacauucgaa ccauaccgaa agcuaaaauu caaccuagac     1080

aguguaaaua uagacgaccu aucccgugaa guacuugaua aacuagcaga uauucucaca     1140

cugaauaccg aacgagaagg aaucgaagac gcaauuagac auaaccuccc caaucaauuu     1200

accgaaggac aaauaucaga aaucauuaaa guacgaaaau cccaaagcac cgcauuuaac     1260

aaaggauggc acaguuucag cgcaaaauua augaacgagc ucauccccga acucuacgcc     1320

acaagugaug aacaaaugac uauacucaca aggcucgaaa aauuuaaagu aaacaaaaag     1380

uccucuaaaa auacaaagac aaucgaugaa aaagaaguua cggaugaaau auacaaccca     1440

guaguagcua aaaguguuag acaaacaaua aaaauaauaa augcagcagu gaaaaaauac     1500

ggagacuuug auaaaauagu aauagaaaug cccagggaca aaaacgcuga cgaugagaaa     1560

aaauucauag acaaaaggaa caaagaaaac aaaaaagaaa aagacgaugc acuaaaacga     1620

gcagcauacc uuuauaaugg cacggacaaa cugccagacg aaguauucca cggaaacaaa     1680

caacuugaaa caaaaauaag acuuugguau cagcaagggg aaaggugucu auacucugga     1740

aaaccaaucc caauacagga guuaguccac aauucaaaca acuuugaaau cgaccauaua     1800

uuaccacuuu cauuaaguuu cgacgacucc cucgcaaaua aagugcucgu uuaugcuugg     1860

acaaaucaag aaaaagguca aaagacccca uaucaaguga uagauagcau ggacgcagca     1920

uggucauuua gagagaugaa ggacuaugua cuaaaacaga aggggcuagg aaaaaaaaaa     1980

cgggauuauu uacuaacgac cgaaaacaua gacaaaauug aaguaaaaaa aaaauucaua     2040

gaacgaaauu uagucgauac uagguaugca aguagagugg ugcucaacuc cuugcaauca     2100

gcauuaagag aacuugggaa agacacuaaa auaaguguaa uccgaggaca auuuacaagc     2160

caacuaagaa gaaaauggaa aauagacaaa ucaagagaaa cauaccauca ccacgcagua     2220

gaugcacuaa uuauagcagc aucuucacaa cuaaaacuau gggaaaagca ggacaaucca     2280

auguucguag auuauggaaa uaaccaagua guagauaaac aaaccgguga gauauuaagu     2340

guuucggacg augaauacaa agaauuaguc uuccagcccc cguaccaagg cuucguaaac     2400

augaucucua guaaaggauu cgaagaugaa auuuuauucu cuuaccaagu ggauaguaaa     2460

uacaaucgaa aaguaagcga cgcaacaaua uauucaacua gaaaagcuaa aauuggaaaa     2520

gauaaaaaag aagaaacuua cguauuagga aaaauuaaag acauauacuc ucagaacggc     2580

uucgacaccu uuauaaaaaa auacaacaaa gacaagacac aguuccuaau guaccaaaaa     2640

gaucccuuaa caugggaaaa cguaauagaa guaauauuaa gagacuaccc cacaacaaaa     2700

aaaagugaag auggcaaaaa cgacguaaaa uguaacccuu ucgaggaaua uagaagagaa     2760

aaugggcuag ucugcaaaua uagcaaaaaa ggcaaaggua ccccgauaaa aagccuaaaa     2820

uauuacgaca aaaaacuagg uaacuguauc gauauuacuc ccgaaggauc aaaaaacgaa     2880

gucguacuac aaagcuuaaa cccguggagg gcagacgucu auuucaaucc ggaaacacuc     2940

aaauaugaau uacucggacu aaaauauuca gaucuaagcu uugaaaaagg cacaggaaaa     3000

uaucacauaa gccaagaaaa auacgaugua auaaaagaaa aggagggaau aggaaaaaag     3060

agugaauuua aauucacacu auauagaaau gaucugauuc uaauaaaaga caccgcauca     3120

ggagaacaag aaauuuauag auuuuuauca cgaaccaugc cuaacgugaa acacuaugca     3180

gaauugaaac cauaugacaa agaaaaauuu gauaauguac aagaauuagu agaagcauua     3240

ggagaggccg acaaaguagg cagaugcaua aaaggguuaa auaaaucaaa ucuuagcaua     3300

uauaaaguua ggacagaugu auugggaaac aaauauuuug uuaaaaaaga aggagacaaa     3360

ccuaaauuag auuucaaaaa caauaaaaaa uga                                  3393


<210>  5142
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5142
augaccaaug guaaaauacu ugguuuagau auuggaauug cuaguguugg agucggaaua       60

auagaagcaa aaacagguaa aguaguccac gcaaacucac gccuauucuc agccgcaaac      120

gccgaaaaca augcagaacg acgaggauuc agaggaagua ggcgacucaa ccgaagaaaa      180

aaacacagag uuaaacgugu ucgcgaccua uuugagaaau acggcauugu aacagacuuc      240

cgaaacuuaa accuaaaucc uuaugagcuc agagucaaag gacugacaga acaauuaacc      300

aaugaggaac uauucgccgc acuccgaaca auaucgaaac gcaggggcau aaguuacuua      360

gaugacgcag aggaugacag cacagguagu acagauuaug cuaagaguau ugaugaaaau      420

agaagauuau uaaaaaccaa aaccccagga cagauacagc uugaaagauu agagaaguac      480

ggacagcuaa gaggcaacuu caccguauac gaugagaaug gagaagcuca cagacuaaua      540

aacguauuca gcacauccga uuacgaaaaa gaagcaagaa aaauccuaga aacacaagcg      600

gauuauaaca aaaaaauaac agcagaauuc auagacgacu auguagaaau ucucacucaa      660

aaaagaaaau acuaccaugg gccgggaaac gaaaagucaa ggacggauua uggaagauuc      720

agaacagacg gaaccacuuu ggaaaauauu uucggaaucc ucauuggaaa gugcaacuuc      780

uauccggaug aauauagggc aucuaaagcc ucauacacgg cgcaagaaua caacuuccua      840

aacgaccuaa acaaccugaa aguuccaaca gaaacaggua aacuauccac agaacaaaaa      900

gaaucacucg uugaguuugc aaaaaacaca gcaauucuag guccagcaaa acuacucaag      960

gaaauagcaa aaauacucga cugcaaagua gacgaaauca aaggauauag agaggaugau     1020

aaaggaaaac cggauuuaca cacauucgaa cccuaccgaa aacucaaauu uaaccuagau     1080

ucagucaaua uugacuacuu gucuagagaa guacuagaua aauuagcaga uaucuuaaca     1140

uugaacaccg aacgagaggg aauagaagau gcaauaaagc gaaacuuacc caaccaauuc     1200

acugaggaac aaauaucaga gauuaucaac guccgaaaaa gccagucuac agccuuuaau     1260

aaaggauggc auaguuucag ugcuaaacug augaaugaau uaauaccaga acucuacgcu     1320

accagugacg aacaaaugac aauacucacg agacuagaaa aauucaaagu aaaaaaaaaa     1380

agcuccaaaa auauaaaaac aauagacgaa aaagagauca cagacgaaau auacaaucca     1440

gucguagcaa aauccguacg ucaaacaaua aaaauaauaa augcagccgu aaaaaaauac     1500

ggagauuuug acaaaauugu uauagaaaug ccccgagaua aaaacgcaga cgaugagaaa     1560

aaauucauaa acaaaagaaa caaagaaaac aaaaaggaaa aagacgacgc ccuuaagaga     1620

gcugcuuacc uauacaacgg uacugauaaa cuaccagacg aaguuuucca uggaaauaaa     1680

caacuagaaa caaaaauucg auuaugguau caacaaggcg aacgaugcuu auauucagga     1740

aaaccaauac caauacagga acuaguacac aauaguaaca auuuugaaau agaccacaua     1800

cuaccacuau cacuaucauu ugacgacagc cuggcaaaua aaguauuagu auacagcugg     1860

acaaaccaag aaaaaggaca aaaaacaccu uaucaaguaa uagacucaau ggaugccgcg     1920

uggucguucc gcgaaaugaa agaguacgua cuaaagcaaa aaggcuuggg uaaaaaaaaa     1980

agagacuauc uauuaacaac agaaaacaua gauaaaauag aaguuaaaaa aaaauucaua     2040

gagagaaacc uaguagauac aagauaugca aguagaguag ugcuaaauuc auuacaaagu     2100

gcauuaagag aacuaggaaa agacacaaaa guaagcguag uucgaggaca auucaccucc     2160

caauuaagga gaaaauggaa aauagauaaa ucaagagaga cauaccacca ccacgcagua     2220

gaugccuuaa uaaucgcugc uucaagccaa cuaaaacugu gggaaaagca agacaaucca     2280

auguucgucg acuacggaaa uaaucaagua gucgauaaac aaacagguga aauccuauca     2340

gucagcgaua acgaauauaa agaauuagug uuucaaccgc ccuaccaagg uuuuguaaac     2400

acaaucagcu caaaaggauu cgaagacgaa auucuuuuca guuaccaggu agauaguaaa     2460

uacaaucgaa aagucucaga cgcaaccauc uauucaacac gaaaagcgaa aauagguaaa     2520

gacaagaaag aggaaacaua cguauuaggu aaaauaaaag acauauacag ccagaaugga     2580

uuugacaccu ucguaaaaaa auauaacaaa gauaaaacac aauucuuaau guaucaaaaa     2640

gacccucuaa caugggaaaa cguaauagaa guaauccuag gggacuaccc aaccacaaaa     2700

aaaucagaag auggcaaaaa ugaugugaaa ugcaacccau ucgaagaaua cagaagagaa     2760

aauggccuaa ucuguaaaua uucgaaaaaa ggcaaaggaa ccccaauaaa guccuuaaaa     2820

uacuacgaca aaaaauuagg aaauugcaua gacauuacac cagaagaauc acguaacaaa     2880

guuauccucc aaaguauuaa cccguggcga gcagauguau auuucaaucc ugagacauug     2940

aaauaugaau uaaugggauu aaaauacagu gacuuaaguu ucgaaaaagg cacuggcaac     3000

uaccacauaa gucaagaaaa auacgaugca auaaaagaaa aagaaggaau aggcaaaaaa     3060

ucagaauuca aauuuacacu uuaccgaaac gauuuaauau uaauaaaaga uauagcaagc     3120

ggagaacagg aaauauacag guuccuaucc agaacaaugc cuaacgugaa ucauuacgua     3180

gaauugaaac cauacgauaa agaaaaauuc gauaauguuc aagaacuagu ugaagcccua     3240

ggugaagcug auaaaguagg aagaugcauu aaaagucuaa acaaaccaaa uauaagcauc     3300

uacaaaguua gaacagacgu auuagggaau aaauacuuug uaaaaaaaga aggagauaaa     3360

ccaaaauuag auuuuaaaaa caacaaaaaa uga                                  3393


<210>  5143
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5143
augagcaacg gcaaaauauu aggcuuagac auagguguag caucagucgg uguaggaauc       60

auagacgcaa aaacagggaa aguaauacau gccucaagca gacuauucag cgccgcaaac      120

gcagagaaua acgcagaaag aagaggauuu aggggagcaa gacgauuaac aaggagaaag      180

aaacaucgaa uuaaaagggu aagagacuua uucgagaaau augaaauagu aaccgauuuu      240

uccaaucuua auuuaaaucc cuaugaacug cgcgucaaag gcuuaacaga acaauuaaca      300

aaugaagaau uauucgcggc auuacgaaca auagcaaaga gacgcgggau auccuauuua      360

gaugaugcgg aagacgauag uacagguuca agcgacuacg caaaaaguau agaagaaaac      420

cgacgucuac ucaagaauau gaccccagga cagauacaau uagaaagacu ggaaaaauau      480

ggacaauuaa ggggaaauuu caccguauau gaugaaaaug gagaagcgca cagauugaua      540

aacgucuuuu caacaucaga uuacaagaac gaagcuagaa aaauacuaga aacgcaauca      600

aauuauaaua aacaaaucac agaugaauuu auugaagacu acauagaaau ccuaacucag      660

aaaaggaaau acuaccacgg accuggaaau gaaaaaucaa ggacugacua cggacgauau      720

aggaccgaug guacaacauu agaaaauauc uucgguauuc uaauaggaaa augcacauuu      780

uacccggacg aauacagaac cucaaaagcc ucauacacag cccaagaaua caauuuccua      840

aacgacuuaa acaaccuaaa aguaccuaca gaaacaggaa aauuaucaac ugagcaaaag      900

cauuaccucg ucgacuucgc uaaaucauca aaagcauuag gaguagcgaa guuacuuaaa      960

gaaaucgcga agauagucga auguuccgua gaugauauca agggauauag agaagacaac     1020

aaagggaaac cggaucuaca cacguucgaa ccauaccgaa aacuuaaauu caauuuaagu     1080

uugaucgaca ucgacgaacu aagccgagaa acccuagaua agcuagccga uauucucaca     1140

uuaaacacgg agagagaagg aauagaagac gcaauaaaaa gaaaucuacc aaaucaauuc     1200

acagaagaac aaauagugga acugguaaac guaagaaaga accaaucguc agcguucaac     1260

aaagguuggc acucauuuag uguaaaauua augaaugaac ucauaccaga acucuaugca     1320

aguucggaag aacaaaugac aauuuuaacg agacuagaaa aauucaaagu uaacaaaaag     1380

uccagcgaaa acacaaagac aauaaacgag aaagaaauaa cagaugaaau auacaacccc     1440

guaguagcaa aauccguguc acaaacagua aaaauaauaa augcagcagu aaagaaauau     1500

ggcgacuuug acaaaauagu aauugaaaug ccuagggaca aaaacgcgga ugacgaaaag     1560

aaauucaucg acaagaaaca aaaagaaaac gcuaaagaag uagaagaugc acuaaaaagg     1620

gcggcauauc ucuauaacgg aaccgauaaa uuacccgacg gaguuuucca cgguaacaaa     1680

caauuaaaaa caaaaauccg acuaugguac caacaaggug aaagaugucu guacagugga     1740

aaaccaauau caauccaaga ccucguucau aacucaaaca acuucgaaau agaccauaua     1800

cucccauuaa gucugagcuu ugaugauuca cuagcuaaua agguguuagu guacgcaugg     1860

accaaucaag aaaaaggaca gaaaacgccc uaccaaguca uagacagcau ggaugcagca     1920

uggaguuucc gagaaaugaa agacuacguu uuaaaccaaa aaggaauagg aaagaagaaa     1980

agagaauacu uauuaacaac ggagaacaua gauaaaauag aaguuaaaaa gaaauuuaua     2040

gaaagaaacc ugguagacac acgauaugca agcagaguag uucuaaauuc uauacaagca     2100

gcacuaaaag aacucgauaa agauacgaaa guaucgguga uacgaggaca auuuacaucg     2160

caacucagac gaaaauggaa aauugauaaa agcagagaaa cauaccauca ucacgcagua     2220

gacgcauuaa uuauugcagc gucuucccaa cuaaaacuau ggcaaaaaca agaaaaucca     2280

auguucguag auuacggaga gaaucaaguc gucaauaaag aaaccgguga aauauuauca     2340

guaucagaug acgauuauaa ggagcuaguu uuucaaccac ccuaccaagg guuugugaac     2400

acuauaucau cuaaggcuuu cgaagaugaa aucuuauuca guuaucaagu agacagcaaa     2460

uauaaccgca aaguaaguga ugcaacuaua uauagcacaa gaaaagcaaa auuaggcaaa     2520

gacaagaaag aagaaacaua cguacuugga aaaauaaaag acauauacag ucaagacgga     2580

uuugaugcgu uuauaaaaag auauaagaaa gauaaaacac aauuucuuau guaucaaaaa     2640

gacccaguaa ccugggaaaa cgucauagaa guaauacuac gagacuaucc aucagaaaaa     2700

uugucagaag acggaaagaa aaacguaaaa uguaacccau uugaagaaua caggcgagaa     2760

aacggauuga uaugcaaaua uagcaagaaa ggaaaaggga caccaauaaa aucauugaaa     2820

uauuaugaca agaaauuagg aaauuguaua gacauaaccc caaaaggcag caaaaacgag     2880

guaguacuac aaagccuuaa cccguggcga gcugaucuau acuucaaucc agacaccuug     2940

aaauaugaac ucaugggacu aaaauacagu gacuugagcu uugaaaaagg aacuggaaaa     3000

uaucauauua gucaagaaag auaugacgaa auaaaaguca aagaagguau agggaaaaac     3060

agcgaguuca aauucacacu auauagaaau gaucugaucc ugauaaaaga uacggaaucc     3120

ggagaagaag aauuauaccg auuucuaucc agaacuaugc ccaacguaaa acauuacgua     3180

gaacuuaaac ccuacgacaa agaacgauac gacggugguc aagaauuaau gaaaguauuu     3240

gggaauguag cgaauggagg ucaaugccuu aaaucucuaa acaaaucuaa ccuaucaauc     3300

uacaaaguaa ggacggaugu auuaggcaau aaauucuuua uaaagaaaga aggagacaaa     3360

ccaaaauuag acuuuaaaaa caauaagaaa uga                                  3393


<210>  5144
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5144
augacaaaug gaaaaauauu aggucuagau auaggaauag cgucgguggg aguaggaaua       60

auagaagcga aaacuggaaa aguaguacau gcaaacagcc gguuauucag ugcugccaac      120

gcugaaaaca acgccgaacg cagagguuuc cguggaucaa ggagauuaaa cagaaggaag      180

aaacaucgag uaaaaagagu acgcgacuua uucgaaaaau acggaauagu aaccgauuuc      240

agaaaccuga aucuaaaccc auacgaauug agaguaaaag gacuaacgga acaacucaaa      300

aaugaagaac uuuuugcagc acugagaaca auaagcaaaa gacgaggaau aagcuaccua      360

gacgacgcag aagaugacag cacgggaagc accgacuacg caaaauccau agaugaaaac      420

agaagacuac ucaaaaauaa aacaccagga caaauccaau uagaacgacu ggaaaaauau      480

ggacaacuac gaggaaacuu cacaguuuac gaugaaaaug gugaagcaca cagauuaaua      540

aauguguuuu caaccucaga cuaugaaaaa gaagccagga aaauacuaga aacacaagca      600

gauuauaaca agaaaauaac ggcagaguuc auagaugauu auguagaaau ccuaacucag      660

aaaagaaaau acuaucacgg uccaggaaau gaaaaaucga gaacagacua cggacgauuc      720

agaacagacg ggacaacccu agaaaacaua uuuggaauac uuauugggaa auguaauuuc      780

uauccagacg aauauagagc cagcaaagca ucauauaccg cacaagaaua caauuuucua      840

aaugauuuaa auaaucucaa aguaucaacu gaaacaggaa aacugaguac ggaacaaaaa      900

gaaagccuag ucgaauuugc caaaaauaca gccacacuug gaccugcgaa acuacuaaaa      960

gaaauagcaa aaauauugga uugcaaagug gacgaaauua aaggauauag agaagacgac     1020

aaaggcaaac ccgacuuaca uacuuucgaa ccguacagaa aacucaaauu caacuuagaa     1080

agcaucaaua uagacgaucu aagccgagaa guaauagaca aauuagcaga uauucugaca     1140

cucaauacgg aaagggaagg aauagaagau gccauuaaac gaaaucuacc aaaucaauuc     1200

accgaagaac aaauaaguga aaucaucaaa gugcgaaaaa gccaaaguac ugccuucaac     1260

aaaggcuggc auaguuucuc agcaaaacug augaacgaac ucauuccaga auuguaugcg     1320

acauccgacg aacaaaugac cauauuaaca agacuagaaa aauuuaaagu gaacaagaaa     1380

aguuccaaaa auacaaaaac uauagacgaa aaagaaguaa cagacgaaau auauaaccca     1440

guaguagcaa aaucgguaag acaaacgaua aaaauaauaa augcugcugu uaagaaauau     1500

ggagacuucg acaaaauagu aauagaaaug ccaagggaua aaaaugcuga ugaugagaag     1560

aaauucauag acaaaagaaa uaaagaaaac aagaaagaaa aagaugaugc guuaaaacga     1620

gcagcguauu uauauaacuc uucggauaaa cucccagacg aaguauucca uggaaacaaa     1680

caauuagaaa caaaaauaag auuaugguac caacaaggug aaaggugcuu auacucaggc     1740

aaaccaauaa guauacaaga auugguccac aacucaaaca acuuugaaau agaucacaua     1800

cuaccacuaa gccuaucauu ugacgauucc cuagcuaaua aaguauuagu uuaugcaugg     1860

acgaaccaag agaaaggaca gaaaacgcca uaccaaguca ucgacuccau ggaugcggcc     1920

uggagcuucc gagaaaugaa agacuacgua cuaaaacaaa aaggacuagg uaaaaagaaa     1980

cgggauuacc uauuaacgac agaaaacaua gauaaaauag aggucaaaaa gaaauuuauu     2040

gaaagaaauc ugguagacac acgauaugca ucaaggguag uacuaaacag ccuacaauca     2100

gcuuugagag agcucgguaa agacacaaaa guaagcguag uaagaggaca auucaccagc     2160

caacucagaa gaaaauggaa aauagacaaa agucgagaga cauaucauca ucaugcugua     2220

gacgcauuga uaauagccgc cucaucacaa uuaaaacuau gggaaaaaca agacaaccca     2280

auguucgugg auuacggcaa aaaccaagua gucgacaaac aaacugguga aauuuuauca     2340

guuagugaug augaauacaa agaauuagua uuucaacccc cauaccaagg auucgucaac     2400

accauaagcu caaaaggauu ugaagacgaa auauuauuca gcuaccaagu agacagcaaa     2460

uacaaccgca aaguaaguga cgccacaaua uacucuacga gaaaggcaaa aauaggaaaa     2520

gauaagaaag aagaaaccua uguacuagga aagauaaaag acaucuacag ucaaaacggu     2580

uucgacacau ucaucaagaa auauaacaaa gacaaaacac aauuuuuaau guaccaaaag     2640

gacagccuga cuugggaaaa uguuauugaa guaauauuga gagauuaccc aacuacaaag     2700

aaauccgaag acggcaaaaa cgauguuaaa uguaauccuu ucgaagaaua cagaagagaa     2760

aauggauuaa uauguaaaua cagcaagaaa ggaaaaggaa cacccauuaa auccuuaaaa     2820

uauuacgaca agaaauuagg aaacuguauc gacaucacac cagaagagag cagaaacaaa     2880

guaauucuac aaucaaucaa ucccuggaga gcagacguau acuucaaucc agaaacuuua     2940

aaauacgaac ucaugggacu aaaauauuca gaucuaucau uugagaaagg aaccggaaac     3000

uaccauauau cccaagaaaa auacgaugcu auaaaagaaa aagaaggaau aggcaagaaa     3060

agugaguuca aauuuacguu auaccgaaac gauuuaauac uaauaaaaga cauagccuca     3120

ggagaacaag aaaucuaucg auuccuauca agaacaaugc ccaauguaaa ccacuacguu     3180

gaauuaaaac cuuacgacaa agaaaaauuu gacaauguac aagagcuugu ugaagcacua     3240

ggagaagcag auaaagucgg aagauguauu aagggacuaa auaaacccaa uauaucuauc     3300

uauaaaguaa gaacugacgu auuaggaaac aaauauuuug ucaagaagaa aggcgacaaa     3360

ccgaaacucg auuucaaaaa caacaagaaa uga                                  3393


<210>  5145
<211>  3393
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5145
augacaaacg gcaaaauauu aggacuugac auaggaauug caucugucgg aguuggaaua       60

auagaagcaa aaacaggcaa aguuguucac gccaacucac gacuuuuuuc agcagcaaau      120

gcugaaaaua augcagaaag gaggggauuc cgaggaucuc gaagauuaaa uagaagaaaa      180

aaacaucgag uaaaacgagu acgugaccua uucgagaaau acgguauagu uaccgacuuc      240

aggaauuuaa accugaaccc auacgaacug agagugaaag ggcuaacaga acaacuaacu      300

aacgaagaac ucuucgcggc ucuaaggaca auauccaaaa gaagaggaau aaguuauuug      360

gacgaugcag aagacgauuc aacaggaagc acagauuaug caaaaucaau ugacgaaaac      420

agaagauugc uuaagacaaa aacccccggc caaauacaau uagaaagauu agaaaaauac      480

gggcaacuga gaggaaauuu cacagucuac gacgaaaaug gagaagcaca uagacugaua      540

aauguauuca guacuagcga cuaugaaaaa gaagcaagaa aaauacuuga aacucaggca      600

gacuacaaua aaaaaauuac agcagaauuc auagacgauu acguagaaau acuaacccaa      660

aaaagaaaau acuaucacgg uccuggaaau gaaaaaucaa gaacugauua cgggcgguuc      720

agaacagacg gcacgacacu agagaacaua uuugggauau uaauagggaa auguucauuc      780

uacccugaug aauauagagc uucaaaggca agcuacacug cacaagaaua caauuuccua      840

aacgaucuaa auaaucuaaa aguccccaca gagacaggaa aauuaucgac agaacaaaaa      900

gaagcauuag uagaauucgc aaaauccaca gccacauugg guccagcaaa auuauuaaaa      960

gaaauagcaa aaauacucga uuguaaaguc gaugaaauaa aaggguauag ggaagacgac     1020

aaaggaaaac cagauuuaca cacauucgaa ccauaucgca aacuaaaauu caacuuagau     1080

ucugucaaca uugacuacuu aagccgagag gugcuagaua agcucgcggg gauacuaaca     1140

cugaauacag aaagagaggg aauagaagau gcaauaaaaa gaaacuuacc aaaccaauuc     1200

acagaagaac agauaucaga aaucaucaac guaaggaaaa gucaaucaac ggcauucaau     1260

aaagguuggc auucguuuuc cgcaaaauug augaacgagc uaauaccaga gcucuacgca     1320

acaucagacg aacaaaugac aaucuuaaca agauuagaaa aauucaaagu caaaaaaaaa     1380

ucaaguaaga acauuaaaac aaucgacgaa aaagaaauua cagacgaaau cuauaacccg     1440

gugguagcaa aauccguuag acaaaccauu aaaauaauaa acgccgcagu aaaaaaguac     1500

ggagacuuug auaaaauugu uauagaaaug ccaagagaca aaaaugccga cgaugagaaa     1560

aaauucauaa acaaaaggaa uaaagaaaau aaaaaagaga aagacgacgc auuaaaacga     1620

gccgcuuacu uauauaacgg aacggauaaa cuaccugacg aagucuucca cggcaauaaa     1680

caacuugaaa caaaaauccg acuaugguau caacaagggg aaagauguuu auauucuggc     1740

aaaccuauac caauacaaga acuaguacac aauucaaaua acuuugaaau agaucacaua     1800

cuccccuuau cacuaucauu ugaugacagc cuagcuaaca aaguacuagu auacucaugg     1860

acuaaucaag aaaaggguca aaaaacacca uaucaaguca uagacaguau ggacgcagca     1920

ugguccuuuc gagaaaugaa agaauaugua cuaaaacaaa aaggacuagg gaaaaaaaaa     1980

agagacuauc uauuaaccac cgaaaauaua gauaaaaucg aaguaaaaaa aaaauucaua     2040

gaaagaaauc ucguagacac gagauaugca ucaagagucg uacuaaacag ucugcaaagc     2100

gcuuuaagag aacuagguaa agacaccaaa gucucagucg ucagagguca auucacaagu     2160

caauuaagaa gaaaauggaa gauagauaaa ucaagagaaa cauaucacca ucacgcugua     2220

gacgcacuaa uaauagcagc aaguucacaa uuaaaacuau gggaaaaaca agacaaucca     2280

auguucgugg auuaugggaa uaaccaagua guagacaaac aaacagggga aauccugagu     2340

guauccgaua acgaauauaa agaauuagua uuucaacccc cuuaucaagg auuugucaac     2400

acaauaucaa gcaaagguuu ugaagacgag auacuuuucu cauaccaagu agacucgaaa     2460

uacaaucgaa aaguguccga cgccacuaua uauucuacca gaaaagcaaa gaucggaaaa     2520

gauaaaaagg aagagacuua cguauuagga aaaauaaaag auaucuauag ucagaacgga     2580

uuugauacuu uuguaaaaaa auauaauaaa gauaaaacgc aauuccuaau guaucagaaa     2640

gauccauuaa caugggaaaa cguuauugaa guaauccuag gugacuaccc aacaacaaaa     2700

aaaucagaag auggaaaaaa cgacguaaaa uguaacccau ucgaagaaua cagaagagaa     2760

aacggacuaa uauguaaaua uucaaagaaa gguaaaggca caccaauaaa aucacuaaaa     2820

uacuaugaca aaaaauuagg caacuguaua gacauaacuc cagaagaauc acgaaauaaa     2880

guaauauuac aaaguauaaa ucccuggcga gcagaugucu auuucaaccc agaaacauua     2940

aaauaugaac uuaugggacu aaaguauucu gaucuuuccu ucgaaaaagg gacagggaac     3000

uaccacauau cucaagagaa auacgacgcu auuaaagaaa aagagggcau agguaaaaaa     3060

agcgaauuua aauuuacucu cuauagaaau gaucuaauau uaauaaaaga uauagcuagu     3120

ggagaacaag aaauuuaucg auucuuaucc cgaacaaugc caaacguaaa ccacuauguc     3180

gagcuaaaac ccuacgacaa agaaaaauuc gauaacguac aagaauuagu agaggcacua     3240

ggugaagcag auaaaguggg gcgauguaua aaagguuuaa auaagccaaa cauaaguaua     3300

uauaaaguac guacagacgu acucggaaau aaguacuuug uaaagaaaga aggcgacaaa     3360

ccaaaauuag acuuuaaaaa caacaagaaa uga                                  3393


<210>  5146
<211>  3396
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5146
auggcccaac acguuuuugg ucuugauaua ggcauagcga guguaggaug ggccaucuug       60

ggggagcaaa gaaucauaga uuuaggaguu agauguuuug auaaagcaga gaccgcgaaa      120

gaaggugauc cccuuaaucu aacaagaagg caggcaagau uauuaaggag gagguuauac      180

aggagagccu ggagacucac acaacuuagu agguugcuaa aacgaaaagg auuaauagca      240

gaugccaaau uauuugcaaa agcuccuagu uacggagaua gugcauggga acuaagacga      300

caaggucugg auagacuauu aacaccgcua gaaugggcaa gaguaaucua ucaucaaugu      360

aaacacagag gcuuccacug gaccucaaaa gcagaagagg caaaagcaga cucugacgcu      420

gaaggaggaa gaguaaaaca gggacuugca cacaccaaag cauugaugca agcgaaaaac      480

uauagaagug cggcagaaau gguauuagca gaauuuccag augcccaaag aaacaaacga      540

ggucaauaug auaaagcauu aaguagaguu uuacuaggag aagaauuggc auuauuauuu      600

gcuacacaga gaagacuggg caacccacac gcuucugacu ucuuugaaaa acuuauauua      660

ggagauggag accguaaauc uggauuguuu uggcaacaaa aaccagcucu aaguggugca      720

gaucuacuua aaaugcuugg aaaaugcacu uuugaaaaag gagaauaccg ugcaccaaaa      780

gccucauuua gcguagagcg acaugugugg uugacaagac uaaauaaucu aagaauagua      840

guagacggga gaucgaggcc acuaaacgaa gcugaaagac aagccgcacu ccuacuucca      900

uaucagacag aaacaucaaa auacaaaaca cuaaagaaug ccuuuaucaa agccggucua      960

uggggagaug guguuagauu cggaggacua gcauacccuu cacaagcuca aauugacgcu     1020

gagaaaacga aagacccgga agaccaauuc cuaguuaaau uaccggccug gcaugaauua     1080

agaaaggcau uuaaagcagc ugggcacgaa gcauuguggc aacaaaucuc uacaccagcg     1140

uuagauggag auccaacacu ucuagaucaa aucgccacgg uauuaucggu auacaaagac     1200

ggggccgaag uugugcaaca acuaagacaa cucgcauugc cugagccagc agcaucaaua     1260

gcuguacuag agaaaauauc auuugauaaa uuuucaucau uaucacucaa agccuuaaga     1320

agaauaguac cuuuaaugca aaguggacuc agauaugaug aagcaguagc gcaaauaccc     1380

gaguacggac accacaguca aaggauagaa ccaggagccg cuaaacauuu auaucugccc     1440

ccauuuuaug aagcacaaag aaaauacgcg ggaaaaggag aucauauagg guccaugcaa     1500

uuuagggacg augcagauau cccaagaaau ccagucguuc uucgagcucu aaaccaagcu     1560

cguaaaguug ugaaugcauu aauacgugaa uauggaucac caauagcggu uaauauagaa     1620

auggcacgag auuugucaag gccacuugau gaaagaaaua aaguuaaacg agcacaagaa     1680

gaauuuaggg auagaaauga ucgggcaaga agcgaguuug aaagagacuu uggauauaaa     1740

ccuaaagcag ccgccuucga gaaauggaug uuauaccgug aacaacuagg acaaugugcu     1800

uacucccaac aaccucuaga cauacaacgg guacuugaug aucauaauua ugcacaagua     1860

gaucaugcgu uaccuuacuc uagaaguuau gaugauucua aaaauaacaa aguuuuaguu     1920

cuuacacaug aaaaccaaaa caaaggaaau cggacugcau ucgaauacuu aacaaguuuu     1980

ccagaugggg aagauggaga aagauggaga acauucgugg cauggguuca aggaaauaag     2040

gcuuaucgaa uggccaaaag aaauaggcuc cuaagaaaga acuauggggu ggacgaauca     2100

aaaggguuca uagaucgaaa ccuaaaugac acaagauaca uaugcaaauu cuuuaaaaau     2160

uauguagaag aacacuugca gcuggcggca agggcugaug gagauacagc aagaaggugc     2220

guuguuguaa auggacaacu aacagcguuu uuacgagcgc gauggggacu aacuaaagua     2280

agaggagauu cagaccgaca ucaugcacug gaugcagcag uaguugcagc augcacacau     2340

ggaaugguaa aagcgcuugc agauuauucc cgaagaaaag aaauaucguu ucuacaagaa     2400

ggauuuccag auccugaaac uggagaaaua uuaaacccag cagcauucga uagagcaaga     2460

caacauuuuc cagaaccaug gacacauuuu gcacacgaac uuaaagcaag acuauuuaca     2520

gacgacuuag cugcucuacg ggaggauaug caacgauugg guagcuauac aaccgaagac     2580

cuagguagau uaagaacauu auuuguuagu agagcuccac agcgaagauc agggggagca     2640

guacacaagg aaaccauuua cgcccaacca gagucacuaa aacagcaagg gggcguuauu     2700

gaaaagauuu uauuaacauc ucugaaacua caagauuuug auaaauuacu aaauccagaa     2760

ucaaaugacc auuuugucga gccgcauaga aacgagagac uuuacgcagc aauuagacaa     2820

aggcuugagc aauuuggagg cagagcagau aaagcauuug gaccggauaa cuuauuucau     2880

aaaccagaca aaaauaacca gccaacagga ccaguaguca gaucuauaaa guuaguaaga     2940

ggaaaacaga cuggaauccc aauaagaggu gguuuggcua agaacgauuc aaugcuucgu     3000

guugacauau ucacaaaagc aggaaaauuu caucucguuc caguuuaugu acaucauaga     3060

guaaccggau uacccaaucg agcaauagua gcguucaaag augaagacga auggacgcuc     3120

auagacgaau ccuuugcuuu ucuuuucuca guauacccaa acgauuaugu aaaaguaaca     3180

cugaagaaag aacaacaaag uggauauuau ucaggcgcag acagaaguac aggugcaaug     3240

aauuuauggg cgcacgauag agcggcaucu guaggaaaag auggauuaau acgugguauu     3300

gguguuaaaa cagcauuaag uguagaaaaa uucaauguag auguauuagg uagaauuuau     3360

cuagcacccc cagagacgag aucuggucug gccuga                               3396


<210>  5147
<211>  3399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5147
auggcgcaau acguauucgg gcuagauaua ggaauagcca gugucgguug ggcaguauua       60

gaugcgcaaa gaauagucga cuuaggagua agauguuucg auaaagcaga aacagcgaaa      120

gaaggcgauc cacuaaaucu aacaagaagg caagcgaggu ugcuaagacg aagguuauau      180

agacgagcau ggagacuaac acaauuagcg cgucuguuua aaagagaagg auuaauagac      240

gacgccaaac uuuucgcaaa ggcaccaagu uggaccgaaa augcauggga acucagacua      300

agaggacuag accaucuguu aacaccauua gaaugggcaa gagugauaua ucaucuaugu      360

aaacaucgag gauuucauug gacuucaaaa gcugaagaag cgaaagcuga aucagacgca      420

gaagguggua gaguuaaaca gggauuagca cacacaaaag cacuaaugca aguuaagaau      480

uauagaucua cugcagaaau ggugcuugcc gaauuuccag gagcacaacg gaacaaaaga      540

ggccaauaug acaaagcucu aucacgaguc uuauuaggug aagaacuugc uuuacuauuu      600

gcgacucaaa gaagacuagg aaauccccac gcaucugaac auuuugaaca gcuuauacuc      660

ggacacggag auagaaaaag uggacuauuu uggcaacaaa aaccagcguu aagcggugcc      720

gauuuauuaa agaugcuugg aaaaugcaca uucgagaaaa ccgaauacag agcgccaaaa      780

gcuagcuuua gugcugaaag acauguaugg uuaacgagac uuaacaauuu acgcauugug      840

guugauggca gagcacgucc auuaaaugac gcagaaagac aagcagcacu ccuauugcca      900

uaucaaacgg aaacuuucaa auacaaaaca uuaaaagccg cuuucgucaa aguugguuua      960

uggggugaug ggguacguuu ugggggacua gcauacccau cccaagcaca agccgaagcg     1020

gaaaaagcca aggaucccga agaccaauuc cuggugaaac uaccggcaug gcaugaauua     1080

cgaaaagcau uuaaagcagc aggacaugaa gcauuauggc aacaaaucuc caugcccgca     1140

uuagaagaag acccaacuuu gcuagaucag auagcgacug uauugucagu auacaaagac     1200

ggaacggaag ugguacagca acuacgucaa uuagcuuugc cggaacccga ugcaaguaua     1260

gcggucuuag aaaagauuag auuugauaaa uuuucaaguc uuucccuaaa agcucuaaga     1320

agaauagugc cacuaaugca aggcggauua agauaugaug aagcaguggc acaaauacca     1380

gaauauggac aucacagcca aagaauugac ccaggagcga caagacacuu auaucuacca     1440

ccuuucuacg aagcacaacg aaaguaugcg gguaaagggg aucgaauagg aucgaugcaa     1500

uuucgagaag augcagaugu accaaggaau ccugucgucu uaagagcauu aaaucaagcc     1560

aggaaaguag uuaaugcacu aauaagagaa uacggaagcc cuguagcagu aaauauagaa     1620

auggcaagag aucugaguag accacuagau gaaagaaaua aaguaaaacg agcucaagaa     1680

gaauuuaggg aucggaacga uaaagcgcgu ucagaguucg aaagagauuu cggauauaaa     1740

ccaaaaggag cuguguuuga aaaauggaug uuauacagag aacaacucgg acagugugca     1800

uacagccagc aaccacuuga cauacagaga guguuagaug acccuaacua ugcacaagua     1860

gaucaugcac uuccauauag uaggucauau gaugauagua aaaauaauaa gguguuagug     1920

uuaacgagag agaaccaaaa uaaagguaau agaaccccau ucgaauauuu aacaucauuc     1980

ccaggcggug aagacggaga gcgauggaga gcuuuuguag cauggguaca agguaacaaa     2040

gcauacagaa uggcaaaaag aaacagauug uugcgaaaga acuauggaac agacgaauca     2100

agaggcuuca uagauagaaa ccuaaacgac acaagguaca uauguaaauu cuuuaaaaau     2160

uacguugagg aacauuuaca auuagcagca agaacagacg gagauacggc acgacgaugc     2220

gucgucguua auggacaacu aacagcauuu uugagagcaa gauggggacu cgcaaaagug     2280

cggggagaua gcgaccguca ccacgcauug gaugcugcag uuguugcagc auguacccau     2340

gguaugguga aagccuuagc agauuauagc agaagaaaag aaauagccuu ucuucaggaa     2400

gguuucccgg auccagaaac cggagaaauu uugaaccucg cagcguucga ucgagcgcga     2460

gaacauuuuc cagaacccug gccacauuuc gcacacgaau uaaaggcuag auuauuuaua     2520

gaugauuuag uagcacugag agaagauaug caacaccugg guagcuaugc acaagacgac     2580

uuggacagac ucagaacacu auucguaucg agagcaccac aaagaagaaa uggaggagca     2640

guccauaaag gaacuauaua ugcacaaccu gagaaucuca aacaacaagg cucaacaaua     2700

gagaaaauau uauuaacagg auuaaaacua caagacuucg acaaacuauu aaauccagaa     2760

ucaagcgacc acuuuguaga accacacaga aaugaaagau uauaugcagc cauaagaaga     2820

agauuagaag aguucggugg uaaagccgac aaagccuucg gaccagauaa accauucuac     2880

aaacccgaua aaaauggaca gccaacgggc cccauuguaa gaucaauaaa acuuguaaga     2940

gguaaacaaa cuggaauacc aauccgagga ggauuggcua aaaaugauuc aaugcuaaga     3000

gucgaugugu uuaccaaagc aggaaaguuu caucuuguac caguauaugu acaucauaga     3060

guaacuggac ugccagaucg agcaauagua gcauuuaaag acgaagaaga auggaccuua     3120

auagacgaau cauacgcuuu ucucuuuagu guguauccaa augauuuugu augugugcaa     3180

caacgaggua aaccccuaau agaaggauac uacgcaggac uugauagagc aaauggaaau     3240

auuaacgucu gggcgcacga cagagccgca aguguaggua aagacggauu aauaagaggg     3300

auaggaguaa aaaccgcucu aucauuagaa aaacuaaacg uggacguuuu gggaagaaua     3360

uauccagcac ccccagaaac uagacgaggg cuggcuuga                            3399


<210>  5148
<211>  3399
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5148
augaaaaaaa ucuuaggcgu agacuuaggc auaacaucuu ucggguacgc aaucuuacaa       60

gaaacaggua aagacuuaua ucgcugcuua gacaauucug uaguaaugag aaacaauccu      120

uacgacgaaa aaucaggcga gagcagucag ucuauaaggu cuacacaaaa aucaaugaga      180

agacuaauug agaaaagaaa aaaaagaaua agaugugucg cucagacgau ggaaagauau      240

ggaauacuag acuacucaga aacaaugaag auaaaugacc cgaaaaacaa uccgauaaaa      300

aauagguggc aauuaagggc aguagacgca uggaaacgac cacuaucgcc acaagaauua      360

uuugcaaucu ucgcacacau ggcaaaacac agaggauaua aaaguauugc caccgaagau      420

uuaauauacg aauuagaguu agaacucgga uugaacgauc cagaaaagga aucagaaaaa      480

aaagcagacg aaagacgcca aguguacaac gcacuucgcc auuuagaaga guuaagaaaa      540

aaauauggcg gagaaacaau ugcacagaca auacacaggg caguugaagc aggugacuua      600

agaucguaua gaaaccacga cgacuaugaa aaaaugauac gacgagaaga uauagaagaa      660

gaaauagaaa aagugcuauu aagacaggcc gaauuaggcg cguuaggccu ucccgaagaa      720

caagucagcg aauuaaucga cgaauuaaag gcauguauaa cagaucaaga aaugccaacu      780

auugaugaau cacuauucgg aaaauguacu uuuuauaaag acgaauuagc cgcgccagca      840

uauucuuacc uuuaugaucu auauagacua uacaaaaaau uagcagaucu uaauauugac      900

ggauacgaag uaacacaaga agacagagaa aaaguaauag aaugggucga aaaaaaaaua      960

gcacaaggaa aaaaucucaa aaaaauuaca cacaaagauc uaaggaaaau ucuaggauua     1020

gcaccggaac aaaaaauauu cggaguagaa gaugaaagaa uaguaaaagg aaaaaaagaa     1080

ccacgcacau ucguaccguu uuucuuucuu gccgacauag ccaaauuuaa agaauuguuu     1140

gcaucaauac aaaaacaccc ugacgcuuua caaauauucc gcgaauuagc agaaauauua     1200

cagcgaagca aaacaccaca agaggcacua gaccgccuga gggcacuaau ggcgggaaaa     1260

gguauagaua cugacgaccg agaacuuuug gaacuauuua aaaauaaaag aucaggaaca     1320

agagaauuaa gccacagaua uauccuagaa gcccuuccau uauucuuaga ggguuaugau     1380

gaaaaagaag uacaacguau ccuaggcuuu gaugauagag aggauuacuc acgauaucca     1440

aaaucacuac gacaccucca ucuacgagaa ggaaaccuau ucgaaaaaga agaaaaucca     1500

auuaacaacc acgcgguuaa aagccuagcc ucuugggcgu uaggacuaau agcugaccua     1560

agcuggagau acggcccauu cgaugaaaua auacuagaga caacaaggga ugcucuaccu     1620

gaaaagauua ggaaggaaau agauaaagca augagggaaa gagaaaaagc gcuggauaag     1680

auaauaggaa aauacaaaaa agaauucccu ucaauugaca aaagacuugc cagaaaaauu     1740

caacuauggg aaagacaaaa aggacucgac uuguacucag gaaagguaau uaaucucucu     1800

caacuguuag auggaucagc cgauauagaa cauauaguac cacaauccuu aggagggcua     1860

ucaacagauu auaauaccau uguuacacua aaaagcguaa augcagcgaa aggaaauaga     1920

uuaccuggag auuggcuggc agguaauccc gauuauagag aaagaauagg aaugcuuagu     1980

gaaaaaggac uaauagacug gaaaaagaga aaaaaucucu uagcccaauc gcuagacgaa     2040

auauacacug aaaauacaca uagcaaagga auaagagcua cuagcuauuu agaggcauua     2100

guagcacagg uuuuaaaaag guauuauccu uucccagacc ccgaacuccg aaaaaaugga     2160

auaggcguuc gaaugauacc ggguaaagua accucaaaaa cacgaaguuu acuuggaauu     2220

aaaaguaaau cacgagaaac aaacuuccac caugcugaag acgcuuugau auuaucaaca     2280

cuaacaagag gauggcaaaa uagacuccac agaauguuaa gggauaacua cggaaaaucc     2340

gaagcagaac uaaaagaguu auggaagaaa uacaugccuc acauagaagg ccuaacauua     2400

gcagauuaca uugaugaagc auucaggaga uuuaugucaa agggugaaga aagccuguuu     2460

uacagagaca uguuugacac aauaagauca auaaguuauu ggguagacaa aaaaccauua     2520

ucagcgaguu cacauaaaga aacgguguau ucaucaagac augaagugcc uaccuuaaga     2580

aagaauauac uagaagcauu cgauaguuua aaugucauaa aggaucgaca uaaacugacu     2640

acggaagaau uuaugaaaag auaugauaag gaaauuagac aaaaauuaug guuacaccga     2700

auagguaaua caaacgauga gaguuaucgc gcugucgaag aacgagcgac acagauagca     2760

cagauccuca cacgcuauca auuaauggac gcucagaaug auaaagaaau agaugaaaaa     2820

uuccaacaag cacuaaagga auuaauaaca ucaccaauug aaguaacagg gaaacucuug     2880

cgcaaaauga gauuugugua cgauaaacua aaugcaaugc aaauagacag aggauuagua     2940

gaaacugaua aaaauaugcu cggaauacau auaaguaaag gcccaaacga aaaauuaaua     3000

uuuagacgaa uggacguaaa caaugcacau gaacuucaaa aagaaagaag cgggauacua     3060

ugcuaucuua acgaaauguu auucauauuc aauaagaaag gacuaauuca uuauggaugu     3120

cuaagaaguu auuuggaaaa aggccaggga uccaaguaua uagcuuuauu uaacccacga     3180

uuuccagcaa auccaaaagc acaacccuca aaauuuacuu cggacaguaa gauaaagcaa     3240

gucggaauag gaucagcuac aggcauaauu aaagcucacu uagaucuaga ugggcaugua     3300

agauccuaug aaguauuugg aacacuuccc gaaggaucua ucgaaugguu uaaagaagaa     3360

agugguuaug gacgaguaga agacgacccc caccacuga                            3399


<210>  5149
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5149
auggauuuaa auuauagaau aggacucgac aucgguauag ccucagucgg cugggcagua       60

cuagaaaaca auucaaaaga ugaaccaguu agaauugucg accuaggagu acgccuauuu      120

gaagcagcag aagugccgaa aacuggagca gcuuuagcug aaccucgaag ggaagcuaga      180

acuacuagaa gaagauuacg cagacgaaaa cauagauuag aaagaaugaa augguuacua      240

caagaauacg gguuaauaca aacggaagag uuuaugaaac gauaucacag cgcaggucua      300

cccgacgucu auagguuaag auacgaagga cuagagagaa aacuaaaaga cgaagaacua      360

gcgcaaauau uaauucauau agggaaacau agaggcuuuc gcuccacaag gaaagcagaa      420

cuaaaagaca aaaacgauaa agagacuggu gcuguauuau cagcaaccag agaaaacgaa      480

aaacuaaugg aggaacaugg cuaucgaacg gcaggagaaa ugauuuacaa agacgauaaa      540

uuuagaacgg gcugcacaug gaaagaagaa ggauauagau uaacaccuag aaacaaagaa      600

ggagauuaua aaaauacgau gcuaagagca uuauuagagg augagguuaa agucauauuc      660

gauugccaac gaaaauuugg uaaucaaaaa gcaucagaag aacuggaaaa agcauauuua      720

gcaauaauga caucacaaag aaguuucgau augggacccg ggaaacaacc ugauggaagu      780

gcaaguccgu augccaugga agguuucggu gaccgaguag gauuaugugc acuagaacca      840

cgagagaaac aagaaccaag agccccaaaa gcagccuaua ccucagaauu guuuguagca      900

uuacagaaaa uaaaucauuu acgcaucaca gagaaaaaug gaaaaggcag aggauuaaca      960

agcgaagaaa gagaaagacu uagcgaccua cuacauaaaa agaaagaauu aacuuacggc     1020

gcaguaagga aagaauugca auuagaccua cgauauaggu uuaauacuuu gaauuacucu     1080

agcaagaaaa ugacugaaga agagaaaaua aaagaugccg aaaaagccaa auucguauca     1140

augguaagcc acuaugaaua uagaaaaaga augagcggua uguuagaaca auuaucacca     1200

gaagaacaag ccgaccuaau ggaugaagua gggaaaaucu uaacauuaua caaaaaugac     1260

gauacaagau cuagagaauu ucaaaaauua ggacuagauu cugaaauaac agaagaacuu     1320

uuagaacuaa auccaagagg auuucaacau guuucucuca aagcuaugaa gaaauuaaug     1380

ccauauuuaa aagaaggauu aacuuaugac aaagcaugug cagaagcugg auaugacuuc     1440

caaggaagug uacaaaaaga aaggaguaga cuacuaaaag gaaaagaaau aacugauaua     1500

auaaaugaua uaaacaaucc uguaguaaaa cguucaguca gucaaaccgu uaaaguaaua     1560

aaugcuauua uacaaaaaua uggcucacca caagcaguga auauagaauu agcucgugaa     1620

augucaagaa auuaugauga aagaaggaaa auggagaaac aaaugaaaga uagacaauca     1680

gaaaaugaaa gagcaaagaa agaaauucaa gaauuaggaa augccuuucc aacuggacaa     1740

gauauacuga aauacagauu guggcacgac caggauggua uuugcaugua cagcgccaaa     1800

agaauaccac uggcagaacu cuuuacagga gcuuaugaca uagaucauau acuaccauac     1860

agcauaacuu ucgacgauuc auacaggaac aaagugcuag uuacaaguga agaaaauaga     1920

caaaaaggga aucgaauacc uuaugaguau uucggaucag acucagaaag auggagcaaa     1980

uucgaaacau uagucaauuc aagaauuaga gauuaccgaa aacaacaaaa ucuccuaaaa     2040

cagucauuca cagaggaaga aagaaaagag uuuaaacaga ggaauuuaaa ugauacaaaa     2100

uauauaacua ggguuauuua caauaugauu agagagaacu uagcuuuagc accauauaac     2160

ucaccgggaa agaagaaaca aguguuugca guuaacggag ggguuacagc auaccuuagg     2220

aaacgcuggg gaaugucagc aaaagauaga aguauagaua cacaccacgc cagagacgcu     2280

guuguaauag cuugcugcac cgacggcaug auacagaaaa uaucucggaa uauucaaggu     2340

cgcgaacuau uauacgcccg aggguuuaca gcaguggacg uagaaaccgg agaaaaauuu     2400

acgagggaug auuuuaccag agaucaaugg gaugagauau uuggaguaca aguaccaaaa     2460

ccaugggagu auuuuaauga agaacuugac auacgacuag gagaaaaucc aguugaguuc     2520

auagaaacuc auagugaugu cagccgaaaa cuagacuacc ccgcaugguu uuaugaaaau     2580

aauauaauaa gaccaguuuu uguaucaaga augccuaacc gaaaaguaac aggagcugca     2640

cacgcggaua cgauuagauc cccaagacau uaucaagaaa cagguagugu acuaacaaaa     2700

acagcauuaa uagauuuaaa acuaaauaag aaaacgggag aaauagaaaa cuacuauaaa     2760

ccugaaagug auacacuauu auauaaugca cugaaagaac gacuagaacu uuauggaggu     2820

gacggaaaga aagcguucga agaaccauuc uacaaaccga aagcagaugg aucucgagga     2880

aauuugguaa gaaaagugaa aaccuaugac aaacuaaccc uagguguaua cgucaauagu     2940

caacauggua ucgcagccaa ugcaaauggu ucuaugguua ggguagaugu auucagaguu     3000

uccgggaaau acuacuuugu cccaguauau auagcggacg uaguacgcaa acgauuacca     3060

augaaagccg ucguacaagg uaaacaauac auagacugga aagaaaugga agauaaagau     3120

uucauauuuu cauuguacac acgcgauuua auuucuuuca aaaguaaaaa cggcaagaaa     3180

guaacaugcg uagauggaac cucaaaagcc gugaaugaag agauaguaua cuauuacgga     3240

gcgaacauuu caacagcauc auuuagugga aaaucacaca acggaaguuu cgaauuuaca     3300

ucauuaggaa uacaaggauu agaauacuua aagaaauacc aaguggaugu uuuaggaaau     3360

guaucagaag uaaaaaggga gaaaagaaug ggcuuuagau ga                        3402


<210>  5150
<211>  3402
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5150
augggaggcu ucgaagugga ccgaaaaaua uccuuagggc uagauauagg gguuucaagu       60

guaggauaca gcguaauuga cguugauacc ggaaaaguac uugaauuagg aagccgccuc      120

aucaacggaa aaauaggaac agaaaaccag gcaagaagag aaaugagagg aacaagacgc      180

cucauaaacc gaaaaaaaca aagaaggaaa gacacaguga aacuauuuaa ugaguucaaa      240

cuaauugacu caaaaaaaga ugaacacuac uuugaccacu uugcaaacaa cagugacccc      300

uaugaauuac ggguaaaagg uuuaagugaa aaacuuucgc ucagcgaaau agcugacggg      360

uuauaucaua uaguaaaacg gagaggcaua aguuacgacu uaaaagacgc ugacgaagaa      420

uccgaggggu cagaauauga aaauggucua aaaaucaaca auagcgaauu aaaaaaccaa      480

acaccggcac aaauacaacu aaaacgacuc aaugaauacg gagcaauucg uggaaaagua      540

guaauagacg aagaaaaaau ccucuuaaac gucuucccaa ccaaagcaua uuuagaugaa      600

gccaaaagaa uuauugaaaa acaaagagaa uucuauccag gcauucugac ugaagaauuc      660

gaagaaaaau auugucagau acuaaccagg aaacgagacu auuucguagg uccuggcucc      720

gaaaaaagcc gaacagacua cggaaucuac aaagaagacg guagaaccuu aaacaauuua      780

uucgaagaac uaauaggcca ugacaaaaua uacccagaag aacuaagagc aagcggagcu      840

agcuucacag cacagcuauu uaacguauua aacgaucuaa acaaccugca uaucucauca      900

uacgaggacc aaaaauuaac uacagaaaau aaagaacaaa ucauaagcga guuaaagucc      960

acuacaagua auguaaauau gaugaaacua auacaaaaaa ucgccggaug ugaagaaagu     1020

gacauaucag gcuacagaac agauaaaaac uauaaacccg aaaucucauc uaugaggaua     1080

uaccgaaaag uacauaagaa acuacuagaa agugagaucg auaucacgaa uuggccggac     1140

gacuucauaa acaaauuaag uuuuauauua acacucaaca cugaaaaugg agaaauaaga     1200

aaccaacuca aaaacagauu acaaccagaa uaugacuucc uaacagacga acuuauacaa     1260

accauaauag acaauaaaga aaguuucgau auaaaaucaa auaauaaaug gcauagauuc     1320

ucaauaaaaa ccauggaacu acuaauaccg gaaaugauaa aaaggccagu ggaacaaaug     1380

acacuaauaa augaaauggg auuaauaaaa aauuccggaa aaaaguuuuc aaauuacaaa     1440

uacuuaccuu acaagaaaau agcagaagac auauauaacc cuguagcguc gaaaagcgua     1500

agagaagcac uaaaaauagu aaaugcaauc cuaaaagaau auggacauau cgaauauaua     1560

guaguagaga ugccuagaga caaaaacgag gaugaagcaa aagaccaaau agaaaaauuu     1620

caaaaagcca auggacgauu aaaagaugca gcacuugaag aauuuacaga agcaauugga     1680

gacaagagca ccgucgauaa aaaacuagga guccauagau cuaaauuacu auucaaaaua     1740

cgauuauggu accaacaaag aggaauggac uuauaugaag ggaaaccaau aucggcagua     1800

gaccuacuaa acaacccaga uucauuccaa auagaucaca uaauaccaca gagcguaagc     1860

uucgaugauu cauuuaacaa caaaacacua uguaacagcg gauuaaacca aaucaaagac     1920

aaaaaaaccc cguucgaaua uaugaacgaa ggcuaugguc aaggauucga uuccaugaaa     1980

gcuaugguga ugaaaaaucc acgaauagac cacaauaaac guaaaaauua ccuauuuagu     2040

gaaaauguaa gcgacaucga aacuagaaaa agauuuauau cccgaaacuu aguagauacg     2100

agauaugcaa gcagaguugu guuaaacaau uuacaagaau ucuuuaaaga aaaagaaucc     2160

gacaccaaaa uaucaauaau aagaggcaaa uucacaagca accuacgaaa acacuggaaa     2220

auaaauaaaa acagagacac guaucaccau cacgccauag acgccacaau uauagcagca     2280

acaccauuuc uaaauaucug gaaaaaaggc ucaucaaucu uuccaagcaa uguagagagu     2340

gaagacuucu uugauaucaa gaccgggaaa auaauaggaa augacgaauu caauaaauac     2400

gcauaugacg aacccuauaa uggauuuuau aaugaauuaa gaaaugcaac agacagaaua     2460

aaauucaguc accaaauaga uaaaaaaaug aacaggaagg ucagcgauag cacaauauac     2520

aguacacgcg uagcaaaauc accaaaaaau aaaaaagaaa gcgaguauau aguggcaaaa     2580

auaaaaaaua ucuaugaugc ugaugaauac gcaaaauuca aacgaaucua cgacaaagac     2640

aaaacaaaau ucuuauuaaa caaauaugau ccaagaaguu uuaaccaucu agaaaaaaua     2700

augaauacau acccugacuc aauagaaaga acaaugucua auaauaaagu aaaaguggua     2760

agaauuaguc cauucgaacu cuauagacaa gaccauggac cugugaaaaa auauagcaaa     2820

aaaaacaacg gacccgaaau aaaacaacua aaauacuuag auaaaaaagu cgguaguuca     2880

auagacguca caccugauaa cgcaaagaau aagcauguca uucuuaaauc gcucaagcca     2940

uggagaacag acaucuauuu aaaccaugaa acaggagaau acgaaauaau gggaauaaaa     3000

uauucagauu uaaaauucaa uaaaggagaa gguuacggaa uuaaaaaagc aaaauauuua     3060

gagaucaaac auagagagaa aguuagcgac gacucagaau ucauguucac acuuuacaaa     3120

aaaaacagaa uaaagguaga aaacauggag accgaugaau cuguagaaau gcuauucugg     3180

ucacgaacaa uggauaguca gaaaggauau gcagagcuca agccuauuua caaauacaaa     3240

acagaagacg auaacuggcc aauauacgga aacgcaaaaa aacaaauaau uaaaaaacua     3300

auacccaaaa acugcaaaau cuggaaagua aauacagacg uacuaggaaa uccauuuuac     3360

guagaaaaag aaggagaaua cccaaaagac guauuagacu ga                        3402


<210>  5151
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5151
augaauggcc uaguacuagg acuagacauc ggcaucgcaa gcguaggcgu agggauacuc       60

gagaaaaacu caggcaaaau uguucacgcu ucaucgagaa ucuuucccgc ugcaacagcc      120

gauaacaaug ucgaaagaag gaaaaaucga caagcacgaa gacuucaucg acgaaagaaa      180

cauagaggag caagacuuaa agaccuauuc gaauauuaug guuuauuaac agauuuuagu      240

aaaguaucua uaaacuuaaa cccuuaucga cuuagaguag acggauuaga ccaacaacuu      300

acuaaugaag aacuauuuau cgcacuuaaa aauaucguaa aacgcagagg aauuagcuac      360

cuagaugacg cuagcgaaga uggaggaacu gucucaucag acuauggaaa agcaguagaa      420

gaaaauagaa aacuuuuagc cgaacaaaca cccggacaaa uacaacucga aagauucgaa      480

aaauacggcc aauugagagg ugauuucacu guaguagaaa acagcgaaaa augccguuua      540

auaaauguau ucagcacaag cgcauauaag aaagaagcgg agagaauacu acgaaaacaa      600

caagaauuua acaaucaaau aaccgaugaa uuuauagagg acuacuuaaa aauacuaaca      660

ggaaaaagaa aauacuauca cggaccagga aaugagaaaa gcaggaccga cuauggacgc      720

uuuagaaccg auggagcuac acuagacaac auauucggca uacuuaucgg caaauguaca      780

uuuuacccua augaauaucg agcgucgaaa gcaucauaua ccgcacaaga guucaaucuu      840

cucaacgauc uuaauaaucu uacaguccca accgaaacca agaaacuauc cgaagaacaa      900

aagaaaacua uaauagaaua cgcaaaauca gcaaaaaccc ucggagccag uacauuacuc      960

aaauacaucg caaaaaugau agacgcgucg guagaccaaa uaagagggua uagggucgau     1020

gucaacaaua agccggaaau gcacacuuuc gaaguuuaca gaaaaaugca aucacuugaa     1080

accauaucag ugggagaacu aucgagaaac auucuagacg aauuagccca cauauuaacg     1140

cuaaauacag aaagagaagg uauagaagaa gcaauaaaua ccaaacugag agauagcuuu     1200

ucacaggauc aaguacugga gcuaguucag uuuagaaaga acaacagcuc auuauucagc     1260

aaaggauggc auaacuuuuc auugaaauua augauggaau uaauaccaga acuauacgaa     1320

acuagcgaag aacaaaugac aauacuaacc agacuaggua aacagaaaag uaaagaaaca     1380

agcaaaagaa cuaaauauau agaugaaaaa gaaguaacag aagagauaua uaaucccgua     1440

guagcuaaau caguaagaca agcaauaaaa auaauaaacg aagcaacuaa gaaacaugga     1500

auauuugaua auaucgugau agagauggca agagaaaaca acgaagaaga cgccaagaaa     1560

gacuacauaa aacgccaaaa agcuaaccaa gaugaaaaau augcagcaau ggaaaaagca     1620

gcauuucaau acaauggaaa gaaagaauua ccagauaaua uauuccaugg ucacaaagaa     1680

uuagcuacca aaauuagacu auggcaucaa caaggagaaa aaugccuaua uacaggaaaa     1740

ucaauaccaa ucagugaucu cauacacaau caauauaaau acgaaauaga ccacauccua     1800

ccacuaucac uaucuuucga cgauagccua agcaauaaag uacucguucu agcuacagca     1860

aaucaagaaa aaggacaaag aacccccuuu caagcgcuag acagcaugga cgacgcaugg     1920

agcuaucgug aguucaaaag cuacguaaaa gaaagcaaac uauuagggaa uaaaaagaaa     1980

gaauaccugu uaaccgagga agauauuuca aaaauagaag uaaaacaaaa auucaucgaa     2040

agaaacuuag uugacacucg cuauaguucc agaguagugc uaaacgcccu ucaagacuuc     2100

uacaaaaacc auaauuucga uacaacuaua ucagucguca gaggucaauu uacaagccaa     2160

uuaagaagaa aauggggacu agaaaaaucc cgagaaaccu accaccauca ugcaguagau     2220

gccuuaauua ucgcggcuag cucacaacua agacugugga agaaacaaaa uaauccucua     2280

aucgcauaca aagaaggaca guucguugau agucaaacag gcgaaaucau aagucuaacg     2340

gacgaugaau acaaagaauu aguauuuaaa gccccauaug aucacuucgu agauacacuu     2400

agcaguaaaa cuuucgaaga caguauauua uucagcuauc agguugauuc aaaauuuaac     2460

agaaaaauaa gugacgcuac aaucuaugca acacgaaaag caaaacuaga caaagagaag     2520

aaagaauaua ccuacacuuu aggaaaaauu aaagacauau auagucuggg uacaaaaaca     2580

cccucaaaaa caggauuuua caaauuuuug gaucuauaca auaaagauaa aagccaguuc     2640

cugauguucc aaaaagaccg uaaaacaugg gacgaaguca uagagaaaau uauggaacaa     2700

uauagacccu uuaaagaaua ugacaaagcc ggcaaauuag uugauuuuaa cccauucgaa     2760

aaauauaggc aagaaaaugg accaauccga aaauacagca agaaagggaa cgguccggaa     2820

auuaaaaguc uuaaauacua cgauauacua cucggaaaac auaaaaacau uacucccgag     2880

ggaucuagga auacaguugc auuacuauca cuaaauccau ggcguacaga cguauacuau     2940

aacauggaaa caaagaaaua ugaguucuua gggcuaaaau acgcagaccu uccuuuugaa     3000

gaagggggug cauacggaau auccacagaa accuauaaug aauuacgaga aaaagaaggg     3060

auagguaaaa acucggaguu caaauucacg cuauauaaaa acgaccuaau acuaauaaaa     3120

gauacugaaa caaacugcca acaauucuuu agguuuuggu caaggacugg caaagacaac     3180

ccaaagagcu ucgaaaaaca caaaauagaa cuaaaaccau augaaaaggc aaaauuugaa     3240

aaaggagaag aacuugaagu ucuaggcaag guaccaccca gcucuaauca auuucagaaa     3300

aauaugcaaa ucgaaaacuu aucuauuuau aaagugaaaa cagauguauu aggaaacaag     3360

cauuucauaa agaaagaagg agauaaaccg aaacuaaaau ucuga                     3405


<210>  5152
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5152
augaauggcu uaguauuagg ccucgacaua ggaauagcgu cugucggagu uggaauccua       60

gagaaaaacu ccgggaaaau aauucacgca aacucacggc uauucccagc cgcaaccgcu      120

gacaauaaug uagaaagaag aaagaaucgc caagcucgaa gacucaacag acgaaagaaa      180

caccgaggcg uacgauucca agaccuauuc gaagacuaug gacuacuaac agauuucagu      240

aaagucagca uuaacuugaa uccguauaga uuacgaguag acggacuaaa ccaacaacua      300

acaaaugaag aauuauucau ugcccuuaaa aauauaguaa aacgccgugg aauaucauau      360

uuagacgacg ccagcgaaga cgguggagca guaaguuccg acuacgguaa agccguugaa      420

gaaaauagaa aacuccuagc agaacaaacg ccaggccaga uucagcuaga aagauucgaa      480

aaauauggac aaguaagagg agauuucacg guaguugaaa acggcgaaaa acuacgacua      540

auaaaugucu ucucaacauc ugcauauaag aaagaagcag aaaggauccu ucgaaaacaa      600

caagaguuca auaaccaaau aacagacgag uucaucgaag auuacuuaaa aauucuaacc      660

ggaaaaagaa aauacuauca cggaccugga aaugagaaaa gcagaacaga cuacggaagg      720

uuuagaacag auggaacuac acuagauaac auauuuggua uacuaauagg uaaauguacc      780

uucuaccccg aagaauaccg agcaaguaaa gcaucauaca cagcgcaaga guucaaccuu      840

cuaaacgacc uaaacaauuu aaccguacca accgaaacaa agaaacuuag ugaagaacaa      900

aagaaaguca uuguagaaua cgcaaaagaa gccaaaaccc uaggagcauc cacguuacua      960

aaauacauug cuaaaaugau agaugcauca guugaccaaa uuagaggaua cagaguagac     1020

guaaauaaca aaccggaaau gcacaccuuc gaaguauaua gaaaaaugca gagcuuagaa     1080

acuaucucgg ucggcgaauu aucaagaaau guauuagacg aauuagcaca cauacuaaca     1140

uugaacaccg aacgagaagg aauggaggaa gcaauaaaca caaaacuaaa agauucuuuu     1200

agccaagauc agguacuaga guugguccaa uuccgcaaaa acaacuccag ccucuucuca     1260

aaaggguggc auaauuucag ccuaaaauua augauggaac ucaucccaga acucuacgaa     1320

acuucagaag aacaaaugac aauucucacc cgauuaggua aacaaaaauc uaaagaaaca     1380

ucaaaacgaa ccaaauacau agacgaaaaa gaacuaacug aagagauuua uaauccagug     1440

guagcgaaaa gcgucagaca agcaauaaaa auaauaaaug aagcaacgaa gaaacaugga     1500

auauucgaca acauaguuau agaaauggca cgagaaaaca acgaagaaga cgccaagaaa     1560

gacuacauua aacgacaaaa agcaaaccaa gacgagaaaa augcugcaau ggaaaaagcu     1620

gccuuccagu acaacggcaa gaaagaacua cccgacaaca uauuccacgg acacaaagaa     1680

cuagccacaa aaauacgacu auggcaucaa caaggagaaa aaugccuaua cacaggaaaa     1740

acgauaccca uuagugaucu aauucauaau caauacaaau acgaaauaga ccauauccua     1800

ccccuaucac uaucauuuga ugauucuuua ucaaauaaag uacugguacu ggcgacagcu     1860

aaccaagaaa aaggacaacg aacacccuuu caggcacucg auucaaugga cgacgcaugg     1920

ucuuauagag aauuuaaaag cuacgucaaa gaaaguaaau uauuaggaaa caaaaagaaa     1980

gaauacuuac uaacugaaga agauauaucc aaaauagaag uaaaacagaa auucauagaa     2040

cguaaccuca uagauacacg auacucaucc agaguaguac uaaacgccuu acaagacuuc     2100

uauaaagccc acaaauuuga cacuacuauc ucaguuguac gaggccaguu cacgucacaa     2160

cuacggagaa aaugggguuu agagaaaagc agagaaaccu aucaccauca cgcagucgac     2220

gcacuaauua uagcugcguc aucacaacua cgacucugga agaaacaaaa caacaguuua     2280

auauccuaca aagaagguca auucguggac agcgaaacag gugaaauuau cucauuaaca     2340

gacgacgaau acaaagaacu uguauucaaa gcacccuacg aucauuucgu cgacacgcua     2400

cgcucaaaga aauucgaaga cucaauccua uucucuuacc aaguagauuc aaaauucaac     2460

cgcaaaauau cagacgcaac aauauacgcc acuagaaaag ccaaauuaga caaagaaaag     2520

aaagaauaua cauauacacu agguaaaauu aaagacauau auucauuagg gacgaaagcu     2580

ccauccaaaa cagguuuuua caaauucuua gaccuauaca acaaagauaa aucacaauuu     2640

uuaauguuuc agaaagacag aaaaacaugg gaugaaguaa uagagaaaau aauggaacaa     2700

uauagaccau ucaaagaaua ugauaaaaca ggaaaacugg uagacuuuaa uccauuugaa     2760

aaauacagac aagaaaaugg accaaucagg aaauacucca agaaagggaa uggaccagaa     2820

auaaaaaguc uaaaauacua cgacauacua cuaggaaaac auaaaaauau aacaccagaa     2880

ggaagucgaa auacgguggc ucuacuaucc cuaaaccccu ggagaacuga cguguauuac     2940

aacauggaaa cgaagaaaua ugaauuucuc ggacucaaau acgcagaccu accauuugaa     3000

gaagguggug cauauggaau aucaacagaa acuuauaaug aauuaagaga aaaagaagga     3060

auagggaaaa acagcgaguu caaauucaca uuauacaaaa augauuuaau cuuaauaaaa     3120

gacacugaaa ccaauuguca acagauauuu agauuuugga gccgaaccgg gaaagauaac     3180

ccaaaaucgu uugaaaaaca caaaauagaa uuaaaaccau acgaaaaagc aagauuugaa     3240

aaaggagaag aacuuaaagu gcuaggcaaa guucccccaa gcaguaauca acuacagaaa     3300

aauaugcaaa ucgaaaacuu aaguauauau aaaguaaaaa cugacgucuu aggaaacaaa     3360

cacuucauua agaaagaagg ugaugaacca aaacuuaaau ucuga                     3405


<210>  5153
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5153
augaacggcc uaguauuagg acuagauauc ggaauagcaa guguaggagu aggaauacua       60

gagaaaaacu cuggaaaaau aauacaugaa aacaguagau uauuccccgc ugccacagcg      120

gauaauaaug uagaaagaag aaagaacaga caagcacgaa gacuaaacag acguaagaaa      180

caucgcggag ucagauuuca agaccuguuu gaagacuaug gacucuuaac agacuucagc      240

aaaguaucaa uaaauuuaaa cccauaccga cuacggguag acgggcuuaa ucaacaacua      300

acaaaugaag aacuguucau agcccuaaag aauauuguua aacgaagagg aauaaguuac      360

cuagaugaug cuucggaaga uggaggcaca guaccaucag auuauggaaa agcaguaaaa      420

gaaaacagaa aauuauuagc cgagaaaaca cccggccaaa uacaacuaga aagauucgag      480

aaauacgguc aacuacgagg ugacuucacg guagaagaaa acggagaaaa augucgucuc      540

auuaacguau uuagcaccuc agccuacaag aaagaagccg aaagaaucuu acgaaaacag      600

caagaguuca acaaucaaau aacaaacgaa uuuauagaag acuacuugaa aauacucaca      660

ggaaaaagaa aauauuacca cgguccaggu aacgaaaaau cucgaaccga uuauggaaga      720

uuuagaaccg auggcacaac acuggauaau aucuucggca uucuuauagg aaaauguaca      780

uucuaccccg aggaauauag aguaagcaaa gcgucauaua cugcacaaga guucaauauc      840

cuaaaugauc ucaacaaccu aacgguccca acugaaacaa agaaacuaag ugaagaacag      900

aagaaagaua uagucgaaua ugcuaaagaa acaaaaacac ucggagcaag caccuuauua      960

aaauacaucg caaaaaugau cgacgcauca guagaccaaa ucagaggaua ucgaguagac     1020

guaaacaaua aacccgagau gcauacguuu gaaguauacc gcaaaaugca aagucuagaa     1080

acaauuagcg ucggagaguu aucaaggaac guacuagacg agcuugcaca uauacucaca     1140

cuaaauaccg aaagagaagg aauagaagaa gcuauaaaca ccaaacuaau gggagucuuc     1200

ucacaagacc aagucuuaga auuagugcaa uucaggaaaa acaauaguag ccuuuucuca     1260

aaaggauggc acaacuucuc uuugaaacuc augauggaau uaauaccaga acuauaugaa     1320

acaagugaag aacaaaugac aauacuaacc agacuaggaa aacaaaaauc caaggaaacu     1380

agcaaaagaa cuaaauacau agacgagaag gaaguuacag aggaaauaua uaauccuguu     1440

guagcaaaau cuguaagaca agcaauuaaa auaauaaacg aagccaccaa gaaauauggu     1500

aucuuugaua acaucgugau agaaauggca agggaaaaca augaagaaga cgcaaagaaa     1560

gacuacauaa aaagacaaaa agcaaaccaa gaugagaaaa augcagcaau ggaaaaagca     1620

gcuuuccaau auaacggcaa aaaggaauua ccugauucga uauuccaugg acacaaagaa     1680

cuggcaacca aaauacgcuu auggcaucaa caaggagaaa aauguuuaua cacaggcaaa     1740

aacauaccaa uaucagaccu gauacacaau caauacaaau augaaaucga ccacauacua     1800

ccgcuauccc uuaguuuuga cgacucccua uccaacaagg ucuuaguacu agcgacagcc     1860

aaccaagaaa aaggacaaag gacaccauuu caagcacuug acagcaugga cgaugcuugg     1920

agcuaccgcg aguucaaaag cuaugucaaa gacucaaaac uccuuucaaa caaaaagaaa     1980

gacuaccugc uaacagaaga agauauuucg aaaauagaag uaaaacaaaa auucauugaa     2040

agaaauuuag ucgacacacg auacucauca agaguaguac uaaacgcacu gcaagauuuc     2100

uauaaagcac acaaauuuga uacuacaauu ucaguaguaa gaggacaguu cacaagucaa     2160

cuaagacgga aauggggauu ggaaaaaucg cgugaaacgu aucaucacca ugccgucgac     2220

gcauuaauua ucgcggccag cagccaauua agauugugga agaaacaaaa uaauccccua     2280

auaacauaua aagaaggaca auucguagau agcgaaacug gcgaaaucau cagccuaaca     2340

gaugacgaau acaaagaauu aguuuucaaa gcaccauacg aucacuuugu agacacgcua     2400

aggucaaaaa cuuucgaaga cucuauauua uucucauacc aagucgacuc aaaauucaac     2460

agaaaaauuu cagaugcaac aauauacgcg accagaaaag ccaaacugga caaagaaaag     2520

aaagaguaua cguacacacu uggcaaaaua aaagacaucu auagccuagg uacuaaaaca     2580

cccagcaaaa caggauuuua uaaauuucuc gaccuauaca acaaagacaa aucacaauuc     2640

cuaauguuuc aaaaagacag aaaaacgugg gacgaaguaa uagagaaaau aauggaacaa     2700

uacagacccu uuaaagaaua ugauaaaaca ggaaaacugg uugacuuuaa uccauucgaa     2760

aaauaccgac aagaaaacgg accaauaugu aaauauucaa agaaaggaaa cggaccagaa     2820

auaaaaucac uaaaauauua ugacauacua cuaggcaaac acaaaaauau caccccagaa     2880

ggcucaagaa acacagucgc cuuacucuca cucaaucccu ggagaacgga uguguacuau     2940

aacauggaaa cuaagaaaua ugaauuuuua ggacuaaaau augcagaucu accuuucgaa     3000

aaaggaggag ccuauggcau aucaaccgaa accuacaaua aacugagaga aaaagaagga     3060

auagggaaaa acagugaguu caaauucaca cuguauaaaa augaucuaau acuaaucaaa     3120

gacacagaaa ccaacugcca acaaauauuu agauucuggu cacgaacagg gaaagauaau     3180

ccaaaaucau uugaaaaaca caaaauugaa uuaaaaccau acgaaaaagc ccgauucgaa     3240

aaaggagaag aacuagaagu cuugggcaag gucccaccaa guagcaauca auuucagaaa     3300

aacaugcaaa uagaaaaccu aagcauauau aagguaaaga cagacgugcu aggaaagaaa     3360

cauauaauaa agaaagaagg agacgaacca aaauuaaaau ucuga                     3405


<210>  5154
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5154
augaaguaca ucgaaucagu aaguaucgga uuugacauag gagucggaag cguaggauac       60

gcagugauca auucaaaaac aaaacaaaua cucgaaagcg ggguuuccau auuucccgcu      120

gccgucgcac aaaaaaacgu cgaaaggaga gaaucuagac aaagucgacg acuacgacgc      180

cgaagaguua acagacuaaa cgacuuaaag aaacuacuaa aaaaaaacgg auuuaucccc      240

agcccaaaua aaaacccaua cgaaauuaga guacgaggau uagagcacca acuaucaaga      300

gacgaaauag cuauagcagu uuuacacaua gcaaaacgaa gaggcauauc auacagccua      360

gacgauguag uagacgaaaa cgucacagau uucaaaggua aaaucgcuaa aaaccaagag      420

gcauuaaaaa auaagacucc cggagaaaua caauacgaac gacuucucaa auacggcaaa      480

guaagaggca caguuacaaa cuacguagaa uaugacgaac aaccagaaua cuuucugaau      540

guauucccaa acuccgccua uguagaugaa gcaaaaagaa uacucacuug ccaaaaacaa      600

uauuaccccg agaucacaga cgaucuaaua gaaaauauag ucaaaauuau aucuagaaag      660

agagcauacu auaucggacc aggaaacgaa aaaucaagaa cagauuacgg aauuuaccga      720

acaaauggag aaacacuaga aaauaucuuu gaaauucuua uagggaaaga cucgguuaac      780

ccugacgaau uaagagcacc agcuaacucc cuaacagcac aaguauacaa cuuacuuaac      840

gacuuaacaa acauacgaua caauggauca gaaaggcuaa cuccagaaca aaaacaagau      900

auucuuucug agcuuuuaac aacugaaaaa acaguucaaa ugcuaaaugu aaucgcaaaa      960

aaaguaaaug ugaaaaaaga agaaauaagc gguuucagaa caaaaaaaga ugauaaacca     1020

gauuuccaca cacucgaagu auauagaaaa guucgcaaac auaugcucaa caaagggauc     1080

gauauaaaug aguggccaac ugaaugcuuc gacgaacuag cauuuaucuu caauuuaaau     1140

acagaaauag gggaaauaag aaauagaaua uccgagaaag uugugcccaa auacgacuuu     1200

auaacaaauc aaauagugga ugauuuacua gaagacccca aaauuuuugc cauaaguaca     1260

ucacaaaaau ggcacagauu cucuauuaaa acaaugaaug uacuaauccc agaacucuua     1320

aacggguaua augaacaaau gacuaucuua acgagaaugg gacucuugga agauucuaaa     1380

aacgacuaca guaauauaga caagauagac auaaaaaacg ucacagaaaa uauuuacaau     1440

ccuguaguaa gaaaaucaau caaaaaaugc uuaaagauuu uuaaugaacu auacaaaaaa     1500

uaccccaaua uaucagacgu agucauagaa cuacccagag aagauaaccc agaagacgca     1560

agaaaacuaa uaaaaaaaau gcaaaaagaa aaucugguag aaaaagauac uucacuagaa     1620

gcauuccgca aagaggcaaa ucucucggaa aacggauuaa uagauuuuuu aagaaaaggu     1680

aaaaaccgag cuuuaaaaau cagguuaugg uaccagcaag aaggcaaaug ucuauacacu     1740

ggagaaccaa uacuagccga ggaccuuugc aaaaacgagg caaauuacga cauagaccau     1800

auuauaccca ucucagucuc auucgacgac ucaaucaaua acaaaguacu cugcaguagu     1860

cacuuuaaua gauucgaaaa agaaaaaaaa acaccguucg aauacuucuc gaacaaauca     1920

ucccacuuag guagauacuc uaauuuugac gaauuucgag caucgguuaa cgcuaauaaa     1980

cgaauaucag augucaaacg aaaaaaucug cuaucaaaag aauuauuaag ugaucucgac     2040

acacgcaaaa aauucauagc gcgaaacuua guagauacua gauacgcauc gagaguagug     2100

uuuaacgaac ucacacaauu uuuugcaagu aaaaaagccg acacuaaggu ugcaguaauu     2160

agaggaaaau uuaccucgca acuacgacga gcuuggcaac uaaacaaauc uagagauaca     2220

uaucaccauc acgcaguaga ugcugcauua auugggguaa cacccuuucu uaaaauaugg     2280

agaucaggua aaaccauuua cccaagaaaa augacagaag cccagacaga cuuugaacuc     2340

aacgaaauca caacaaacga cgaauuuaaa gaaagcuacu accaauuacc uuguccacaa     2400

uuucuggauu accuuaccca aaugcaagaa aacuuaaaga uaaacuuuga aguugacaaa     2460

aaaaugaacc guaaaaucuc cgaugcuaug auuuauucau cacgaaaaaa aaaacuauca     2520

aaagacaaga aagaaaaaga auacucacuc ggaacaauua augacauuua uuccgaugca     2580

ggauauagca aauuuaaaaa uauauacaaa aaagauaaag aaaaauuccu aauguaucac     2640

cuagaccaua aaucauucga aauccuagaa gauauaauau cccaauaccc ugauaaagag     2700

gaauacaucg acucggaagg caaaguaaaa caaagggcag uuucaccauu cgaaaucuac     2760

aggcgaaaau augguuacau acguaaguac ucuaaaaaaa acaacggacc caucaucaaa     2820

aacaugaaau acuauaauga aaaccuacca aaaauauaca uugauauaac cccgaaacaa     2880

ucgauagaca aaaaaguuua ucuuacaaau auaucgccau ggagaacuga uguuuacuau     2940

aaccgaaguc aaaauucaua ccauauaaug ggaauuaaau auagcgauuu gagauacauc     3000

gacgggaaau auggaauacc uaaaaacguu uacgagaaaa ucaaacuaag agaaaaaaua     3060

ccuucagaau cagaauuuug cuucucgcuc uauaaaaaug auagaguaaa aauaauagau     3120

cccaauaaaa augaggaaau agaacuaaga uucaauucac gcacacuacc aagcguacua     3180

aauuaugucg aauuuaaacc auuauuccaa gaaagcuaua acucucuaac gcuacuacca     3240

guauucucaa aaacaacgag cgaaggacgc auaauuaaaa aauucacgcg agaagacaau     3300

auuguauaca aaguaaauac agacgaacua gggaacccau acuacauacu uaaagaauca     3360

caaaacccaa aaaacauaau cgauaacuca aacaaaaccg aauga                     3405


<210>  5155
<211>  3405
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5155
augaauggac uuguacuggg cuuagacauc ggaauagccu cagaaggagu cggaauauua       60

gagaaaaauu cagguaaaau cauacaugca aacaguagac uauucccagc agcuacagcc      120

gacaacaaug uagaaagaag aaagaacaga caagccagaa gauuaaauag acgaaagaaa      180

cacagaggag uaagauucca agaucuguuu gaagacuacg gucuacuaac agacuucuca      240

aaaguaucca ucaaucuaaa uccuuaucgc uuacgaguug auggauugaa ccaacaauua      300

accaacgaag aacuauuuau agcauugaaa aauauuguaa agagaagagg aauaagcuau      360

uuagacgacg caagcgaaga cggaggagca guaucgucug acuauggaaa agccguagaa      420

gaaaacagaa aacuacuagc agaacaaacu cccggucaaa uccaauugga acguuucgaa      480

aaauacggac aagucagagg agacuucaca guagucgaaa auggcgaaaa auuacgcuua      540

aucaauguuu ucagcacauc agcuuacaag aaagaagcag aacgcauccu acggaaacaa      600

caagaguuca acaaucagau uacagaugag uucauagaag acuaccugaa aauacuaaca      660

ggcaaaagaa aauauuacca cggacccgga aaugagaaaa gcagaacaga cuauggaaga      720

uuccgaacag acggaacaac auuggacaac auauuuggca uccuaauagg uaaauguacg      780

uuuuacccag aagaauauag agcuagcaaa gcaucuuaca cagcacaaga guucaacuua      840

uuaaacgauc uaaacaaccu uacaguccca acggaaacaa agaaacuaag cgaagaacaa      900

aagaaaguca uaguagaaua cgcaaaagaa gcaaaaaccu uaggcgcuag cacauuacua      960

aaauauauag cgaaaaugau agaugcaagu gucgaucaaa uaagaggaua ucgggucgau     1020

guaaacaaca aacccgaaau gcacacauuu gaaguuuaua gaaaaaugca aagucuagaa     1080

acuauuucag ucggagaacu uucaagaaac guacuagacg aacuagcaca cauacuaaca     1140

cuuaacacag aaagggaagg uauggaagaa gcaauaaaua cgaaauuaaa agacagcuuu     1200

ucccaagauc aggucuuaga acuuguacaa uuuaggaaaa auaauucaag ccuauuuucg     1260

aaaggauggc acaacuuuuc auuaaaacuu augauggaac ucauuccgga acucuacgaa     1320

accucagaag aacaaaugac gauacuaaca cgccucggaa aacaaaaauc aaaagaaaca     1380

aguaaacgaa cuaaauauau agaugaaaaa gaauuaacag aagaaauaua caaucccguu     1440

guugcaaaau caguucgaca ggcaauaaag aucauuaaug aagccacaaa gaagcaugga     1500

auauucgaca acauuguaau agaaauggcu cgagaaaaca acgaagaaga ugccaagaaa     1560

gauuauauaa aaagacaaaa agccaaccaa gaugagaaaa augcagcaau ggaaaaagca     1620

gcauuucaau acaacggcaa gaaagaauug cccgacaaca uauuucacgg acacaaagaa     1680

cuugcaacca aaaucagacu auggcaccaa caaggugaaa aauguuugua cacgggaaaa     1740

acaauaccaa uuagcgacuu aaucuauaau caauacaaau acgaaaucga ccacaucuua     1800

ccacuaagcc ucaguuucga cgacucacua aguaauaaag uccuagcauu agcuaccgcu     1860

aaucaagaaa aaggccaacg aacuccauuc caagcacucg auucaaugga cgacgcaugg     1920

aguuauagag aauuuaaauc auacgucaaa gaccuuaagu cauuaagcaa caagaagaaa     1980

gacuaucucc uaaccgaaga agauauauca aaaauagaag ugaaacaaaa auucauagaa     2040

cgaaacuuag uagacacuag auacagcagc agaguugugu uaaaugcucu acaagacuuc     2100

uauaaauccc accaauuuga uaccacaaua agcguuguaa ggggacaauu uacaucacaa     2160

uuacgccgaa aauggggacu ugaaaaauca agagaaacuu aucaccauca ugcuguagac     2220

gcauuaauaa ucgcagcaag cucccaacuc agauugugga agaaacaaaa uaacccacug     2280

auuacauaua aagaaggaca auuugucgac agccagacug gugaaaucau cucucuuacc     2340

gaugacgaau acaaagagcu aguauuuaaa gccccauacg accacuucgu ugacacucua     2400

uccaguaaaa cguucgaaga cucaauauua uuuucauacc aaguagauag caaauucaac     2460

aggaaaauca gugaugcaac cauauacgcu acaagaaaag caaaauuaga caaagaaaag     2520

aaagaauaua cauacacguu aggcaaaaua aaagacauau auagucuggg uaccaaaaca     2580

ccaaguaaaa caggcuuuua caaauuucuc gaccuauaca acaaagacaa aucgcaauuc     2640

cuaauguuuc aaaaagaccg caaaacaugg gaugaaguaa ucgagaaaau cauggaacaa     2700

uacagaccuu ucaaagaaua cgacaaaaca gguaaguuag ucgacuuuaa cccauucgaa     2760

aaauaccgcc aagaaaauga accgauaaga aaauacucaa agaaagguaa cggcccugaa     2820

aucaaaagcc uaaaauauua cgacauacuu cuaggcaaac acaaaaauau aacacccgaa     2880

ggcucacgaa acacaguagc auuacuaagu uuaaauccau ggaggacgga cguauacuac     2940

aauauggaaa ccaagaaaua cgaguucuua ggccucaaau acgcagaccu accauuugaa     3000

gaaggaggug cauacggaau aucaacagaa acauacaaug aauuaagaga aaaagaagga     3060

aucggcaaaa auucugaguu uaaauucaca uuauacaaaa augaccuaau auuaauuaaa     3120

gacacugaaa cuaauugcca acaaauuuuc agauucugga gucgaacagg aaaagauaau     3180

ccuaaaaguu uugaaaaaca caaaaucgaa cucaaaccgu acgaaaaagc acgauucgaa     3240

aaaggagagg aacuagaagu acuaggaaaa guaccgccaa gcuccaacca acuccagaaa     3300

aauaugcaaa uagaaaaccu auccaucuac aaagucaaga cagauguacu cgguaauaaa     3360

cauuuuauaa agaaagaggg agauaaaccg aaauuaaaau ucuga                     3405


<210>  5156
<211>  3408
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5156
augagcgauc uaguccuugg ucuagacaua gguauaggcu caguaggugu aggaauuuua       60

aauaaaguaa ccggcgaaau aauacauaaa aauucaagga uauuuccagc agcacaagcu      120

gaaaauaauu uggaaagaag aaccaaccga caagggagaa gacuaacaag aagaaagaaa      180

caucggaggg uacgacuaaa ccacuuauuu gaagaaucag gacucaucac ggauuucaca      240

aaaguaagca uaaaccugaa cccguaccaa cugagaguca aaggacucac agcagaacuu      300

ucaaaugaag aauuauucau agcacucaaa aacaugguaa aacaccgugg uauaucauac      360

uuagaugaug caucagacga uggcaacagc agugucggug auuaugccca aauagugaaa      420

gaaaacucua aacaacuaga aacuaaaaca ccuggacaaa uccaacugga aagguaccaa      480

aaauacggcc aauuacgcgg agacuucaca gucgaggaag auggccgcaa acacagacua      540

aucaacguuu uuccaacaag cgccuaccac gccgaagcgc uucgcauauu acaaacacaa      600

caagaguuca auccccaaau aaccgaugaa uuuauuaauu ccuacuuaga aauucuaaca      660

gguaaaagaa aauauuacca cggaccaggc aacgaaaaau cacgaacaga uuauggaaaa      720

uacaccacua aaaaggaugc acaaggacaa uacauaaccc uaaauaacau auucgggauc      780

uuaauaggua aauguacauu uuauccagaa gaauacagag cggccaaagc uagcuauaca      840

gcccaagaau uuaaucuauu aaaugaucua aacaacuuga caguacccac cgaaaccaag      900

aaacucagug aagaacaaaa auaucaaaua auaacauacg ugaaaaauga aaaagccaug      960

gguccagcaa aacuuuucaa auauauagca aaacuauugg ccugugacau agcugauauc     1020

aaaggauaca gaauagacaa auccgacaaa gcggaaauuc auacauucga agccuauagg     1080

aaaaugaaaa cacuagaaac uauagauaua gaacaaauag aaagagaaaa gcuagauaag     1140

uuagcauaug uacugacacu uaauacugaa agagaaggca uucaagaagc acucgaccau     1200

gaguucgcag auggaacauu uucacaagaa caaguagaug aauuaguaca auuuagaaaa     1260

gcuaauagcu caauauucgg aaaaggaugg cauagcuuuu cagucaaacu uaugauggaa     1320

cuaauaccag aacuauacgc cacuucugaa gaacaaauga caaucuuaac ucgacugggg     1380

aaacaaaaga caacaucauc uagcaacaaa acuaaauaca ucgacgaaaa acaacuuacu     1440

gaagaaauau acaaucccgu agucgcaaaa ucaguaagac aagcuaucaa aauaguaaau     1500

gcggcaauca agaaauacgg cgauuucgac aacaucguaa ucgaaauggc cagagaaaca     1560

aacgaagaug augaaaagaa agcuauacag aaaauacaaa aagccaacaa agccgaaaaa     1620

gacgcagcca ugaggaaagc agcgaaccaa uauaauggaa aagcagaacu accccauagu     1680

guauuccaug gucacaaaca acuggcaaca aaaauacgac uauggcacca acaaggagaa     1740

cguugcuuau auaccggcaa aacuauaucc auacaugauc uaauaaauaa ccccaaccaa     1800

uuugagaucg accauauucu accgcuaagu aucacauucg acgacuccuu agccaacaaa     1860

guacucguuu acgcaaccgc uaaccaagaa aaaggacaaa gaaccccaua ucaggcuuua     1920

gauucaaugg acgacgcuug gucauuccga gaacuaaaag cauuuguccg cgaaucuaaa     1980

gcacuaagca acaaaaagaa agaguaccua uuaaccgagg aagauaucuc uaaauucgac     2040

guaaggaaga aauucauuga aagaaaccuu guggacacua gauaugcauc ccguguagua     2100

cuaaaugcuc uacaagaaca cuucaaagca cacaaaauug acaccaaagu aucaguagua     2160

cgaggacaau ucacaucaca acucagaaga cauuggggaa uagacaaaac uagagauacc     2220

uaucaucacc acgcggucga cgcacuaauu aucgcagccu caagucaacu aaaccuuugg     2280

aagaaacaga aaaacacucu aguuuccuau ucugaagacc aacuucuuga uaucgaaaca     2340

ggugaacuca uaucagacga cgaauacaaa gaaucaguuu uuaaagcacc cuaccaacau     2400

uucguugaca cauuaaaaag caaagaauuu gaagacagua uacuuuucuc uuaucaagua     2460

gacuccaaau uuaaccguaa aauauccgac gcaacaauuu augcaacaag acaagccaaa     2520

guaggaaaag auaagaaaga ugaaacauac guauuaggua aaauaaaaga cauauacgcu     2580

uuaggaacaa aaacucccuc aaaaacagga uuuuauaaau uccuugaucu cuauaacaaa     2640

gauaaaaguc aauucuuagu cuaccaaaaa gaucgcaaaa cgugggacga gguaauagag     2700

aaaauaaugg aacaauacag accauucaaa gaaagugaua aaaauggaaa agaagucgau     2760

uucaaccccu ucgaaaaaua ucgaaucgaa aauggaccua uaagaaaaua uuccagaaaa     2820

ggaaaugguc cugaaauaaa aucacuaaaa uauuaugccu ccaaacuugg aaaccacaua     2880

gacauuacac caaaagacuc aaacaacaaa guuguccuac aauccgucag uccauggcgc     2940

gcugauguau auuuuaauaa aacaaccggc agcuaugaaa uacuaggcuu aaaauacgcg     3000

gauuuacaau ucgaaaaagg aacaggaaca uauagaauaa gugaagaaaa auacaacgau     3060

auuaaaaaga aagaaggagu cgauucagac agugaguuua aauuuacucu auacaaaaac     3120

gaccuacucc uuauaaagga uacugaaaca aaagagcaaa gacuauuucg cuuccuaagu     3180

cgaaguaugc caaacaagaa acacuaugua gaacuaaaac cauacuccaa agacaaauuc     3240

gagaaaaacg aauccuuaau agaaauacuc ggaucugcag acaaaagcgg aagauguauc     3300

aaaggucuug cuaaauccaa ccuuucaauc uuuaaaguca gaacagaugu auuaggcaau     3360

caacauauaa uuaaaaacga aggcgacaaa ccgaaauuag auuucuga                  3408


<210>  5157
<211>  3408
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5157
augggaguaa uauuuuugua cgaaaacauu guuaaacccu uuccaucacg auacgacaag       60

gccaucauga aaagauuaaa aggaagacaa acaauggggu auauacuuuc uuuggauauu      120

ggaauagccu cuugcgggua caguguccua gagacagaug cuuuaggagu accauauaaa      180

auaaaaacac ugaauagcau aaucuucaca cgagcagaaa auccuaaaac aggcgccagu      240

uuggcugaac ccagaagaaa auaucguggg gcaagaagaa gaaaacgaag aacaaguuuc      300

cgaaaacaca ggacuagaca auuauucaua acauccgguu uguuauccaa acaagacaua      360

gcaagauacc uaaaucaaca agaaccucac gacgauauau gggaauuaag aguaaaagga      420

uuagaugaac cacucaccaa ccuacaacua uuccaaguau uauacuacuu ugucacacac      480

agaggauuca aaucaaacag aaaagcagaa cuaaaagaua aagauauagg acccguacua      540

aauaguauaa acgcauucaa agaagaaaug caaucacacg acuauagaac aauaggcgaa      600

cuauacgcca augauccaag auuucgugau cgaaaacaua auaaagauua uacaaacggc      660

uuucuaggaa cgauacaaag aucaugguua guggaugaaa uuaaaauguu auuaacgaau      720

caacaaucau uugguaacac ucagcuaaca agcgaguuca uagaauuaua cuuaaacaua      780

uucacuucuc aaagaaauuu ugaugaugga ccugccucag guccauacuc aggagauuua      840

aucgauaaaa ugguaggacc ggacaccuuc aauagaagca gaaaacgcgc cccaagagcg      900

acauauacgu uccaauauuu uucguuucua aguaaauuaa auagcuuacg aauagcacca      960

ucuauuucag caccauuuga aacucucaca guugaccagc gcgcucuaau aacaaaacgc     1020

cuaugggguc cagacguuaa auccgcagau uuaaaauuca aacaaauuag gaugguguua     1080

agccuaccug aaacagcaag auuuaaucuu guaaauuacg gaaauacacc cgaaaaagcc     1140

cuagaaaucg aggacaaaac cacuuucauu ucgauuaaac cacucagaga aauagaaaaa     1200

gcacuaacag gaacaacacu accagucuca cacgaucaac ucgacgcaau agcaagaaua     1260

uuaaccguau auaaaucaga uucuaacaga aaaaccgcau uucacaacca aacuguauug     1320

ucagacgaag uuaucgaaca acugcucccu cuuaauuucu caaaguucgg ccaccucucc     1380

guugaaacga ugcaagagau cauuccauac uuagaacaag gcaugguaua uucugaugcu     1440

gcuacugccg cuggauacga cuuuagaaac aaucaaauag acauggaaua uaucagagau     1500

aacgucacua auccagucgu uaggagagcc guuagcguag caaauaaacu cguaaaagcu     1560

guagugagac gauacgguaa accugauuca auacacauag aacucgcuag agaccuaucc     1620

caaaacuucc aagaaagacg ccaagacgaa guucgacaac agaaaaaugc uagggacaau     1680

gagaaaauag caaaaagacu cgaggaacuu ggaauacccg uaaauggagu aaauaucacu     1740

aaaaugaaac uauacgacga acaacauggu auagaccugu augaccuuuc uacgaacgca     1800

ucaauggcag ucacagauau auuuagugac gauagauacg aaauagacca uauaauaccc     1860

uacucaguca guuuugacga uucuuauaca aacaaagucu uaguaucagc aaaagcaaau     1920

agagagaaaa gagaccgaau accaauggaa guauucggaa augauucaga aagaaggaga     1980

caauuugaaa aucgaguuaa cacaaacaua accaaccuaa ggaagaaaag aaacuuacua     2040

aaagaaacgu uuagcgacga agauaaagcu aaauggaaaa gucggaauau aaacgauaca     2100

cgguacuuaa auagaauacu aaugaacuac uuaucccaaa auguagaauu uacggacgug     2160

cuaucugaug cuaaacacgu aaucgcucuu aauggagcag uaaccgcaaa aauaagaagu     2220

agauggggaa uaacaaaagu aagagcagcu ggcgaucuac aucacgcagu ugacgcaguc     2280

guaaucggcu gcguaacgga cgcguuaaua caagccguaa cucgauaugc aaaauaccaa     2340

gagagaagag auaauaaagc agcagcugaa caggaucugg cagagaacaa uccagaagaa     2400

cucgacgcca aacaauuuag aaagguauuu aaugauuacc cucuaccgug gccagaguuc     2460

cgggaagaau uaaugggucg cguaucuagu gacccaucgc aaauaauggc aggucgacag     2520

uggucacacu auacagcaga ggaaaucagu gaauugaaac aaccauucgu uguaagggua     2580

ccgaaucauc gaauaguagg cccagcaaca uuagacacag uauauggaca gcccaaucca     2640

gacgauggcc uaauaacaca aagaguaagg auaaccgacu uaaaacccca gaaaaacaaa     2700

gacggugaag acauaauaaa agcaggagcu aauguuuacg aacuagcggc cgaugguggc     2760

aauaaacuug uauaugaaag uaucuuagac gcacugcaag uagcgggagg agaugguaag     2820

aaagcauuuc caacaggcag acuucuaguu agaucgccaa uucaagauau uccaguagaa     2880

agaguaagag uuacuucgaa agcgacucua gucguucccg uaggcccaaa aagugucgcg     2940

gcaaaugguu cgauggucag aauagauguc uauaaaacuc cuaagaaaua uguauucguu     3000

ccaauauaug ugagagacac aguaaaagca gaauuacccg auagggccgu agcaccuguc     3060

aaaccguaua gucaauggua ccaauuacaa ccaaaagacc aauucuuauu cucgcuauuu     3120

gcuaaugacu uaauaagauu uaaacaccca agaggaguua guacaaaaga aauucuaacu     3180

gacgguucac aagaaaaggg aaaaagaaca gauguagugg gauauuuuac aggagccgau     3240

auagcaacag cgaguauagc aauagaggga ccagaccguu cuuuuucagc gcguggaauu     3300

ggauuugcaa guuuaaagaa cguggaaaaa cuuacuguaa acuacuucgg agagcuucau     3360

ucagugcacg aaacacaacc aaugcaauuc agaggacaaa gaggauga                  3408


<210>  5158
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5158
augaacggcc uaguacuggg auuagacaua ggaauagcua gcguuggagu agguauacuu       60

gaaaaagaua cuggcaaaau aauacaugcu ucaucaagac uauucccagc ugcaacagca      120

gacaauaacg uagaaagaag aaguaacaga caaggcagaa gauuaaauag gagaaagaaa      180

cacagaagcg uacgcuuaca agaccuauuu gaaucauacg gauuauuaac ugauuucucg      240

aaauuaucga uuaaucuaaa ccccuaucaa cuuagaguac aaggaaugga aaaucaauua      300

acaaaugaag aacuauuuau agcacucaaa aacauaguca aacgacgagg uauuucguau      360

uuagacgacg ccuccgaaga cggggguaca guuuccagcg acuacgguaa agcaguagaa      420

gaaaacagac gacuacuaac agagaaaacc ccaggccaaa uacaacuaga aagauucgaa      480

aaauauggac aguuaagagg ugauuucacc guagaagaaa acggagaaaa acgaagauua      540

auuaacgugu uuucaacuuc ggcauaccga aaagaagcug aaagaauacu acgaaaacaa      600

caagaguuca acaacaaaau aacggaugag uucaucgaag acuaucuaac caucuuaacc      660

ggcaaaagga aauacuauca cggaccgggg aaugaaaaau cuagaacaga uuauggcaga      720

uuuagaacug auggcacuac acuggacaac auauuuggca uauuaauugg aaaaugcacc      780

uucuauccug aagaauacag agccuccaaa gcgucuuaua cagcacaaga auuuaauuua      840

cuaaacgacc uuaauaaccu aacaguacca accgaaacca agaaauuaag ugaagaacag      900

aagaaaacua uaauugaaua cgcgaaauca gcaaaaacuu uaggcgcuuc gacauuacua      960

aaauacauag cuaagaugau agacgcaagc guagaucaaa uacgaggaua ccgaguagac     1020

guaaauaaca aaccagaaau gcacacguuu gaaguauacc gaaaaaugca aucacuagaa     1080

acuaucaaag uagaagaauu accgcgaaaa guauuagacg aacuagccca uauacugaca     1140

cuaaacacag aaagagaagg uauagaagaa gccauuaaca guaaauuaaa agauacauuc     1200

aaccgugauc aaguacugga acuuguacaa uucaggaaaa auaacaguuc acuauuuagc     1260

aaagguuggc acaauuuuuc guuaaaacua augauggaac ucauuccuga auuauaugaa     1320

acgagcgaag aacaaaugac aauuuuaacg agauuaggaa aacaaagauc caaagaaaca     1380

aguaaaagaa caaaauacau agaugaaaaa gaacugacag aagaaauaua uaaucccguc     1440

gucgcgaaau caguuagaca agcaauaaaa aucauaaaug aagcgacaaa gaaauaugga     1500

aucuucgaca auauugucau cgaaauggca agagaaaaca augaagaaga ugccaaaaag     1560

aauuauauua aacagcaaaa agcaaaucaa gaugagaaaa acgccgcaau ggaaaaagcg     1620

gcauuucaau auaacgguaa gaaagaacuu ccagacaaua uauuccaugg gcauauggaa     1680

cuggcaacaa aaauaagauu auggcaucaa caaggggaaa aaugccuaua uacaggcaaa     1740

aacauacaaa ucagcgaccu uauacauaac caauacaaau acgaaauaga ucauauacuu     1800

ccccucagcu uaaguuucga ugacaguuua ucaaacaaag uuuuagucuu agccacagcu     1860

aaccaagaaa aagggcaaag gacaccauuu caagcacucg auagcaugga ugacgccugg     1920

ucauacagag aguucaaauc auacguaaaa gaaaguaaau uacuuggcaa caagaagaaa     1980

gauuaucucc uaacugaaga agauauaagc aaaauagaag ucaaacaaaa auuuauugag     2040

aaaaaccugg uggauacaag auacagcagc agaguagucu uaaaugcguu acaagacuuu     2100

uauaagaaac aucaguucga uacgacaaua uccgucguaa gaggacaauu cacgucccaa     2160

cuaagaagaa aauggaaacu cgagaaaagu agagauacau accaucauca cgcaguagac     2220

gcacuaauua uagcagcaag uagccaacua cgccuaugga agaaacauaa caaucccuug     2280

aucucguaca aagacggaca auuuguagau agugaaacag gagaaauagu aucauuaagc     2340

aaugaagaau auaaagaauu aguuuuuaaa gcuccauaug accacuucgu agauacacua     2400

agaagcaaga aauuugaaga cucaauacug uucucauauc aaguugacag caaauacaac     2460

agaaaaauca gugacgcaac aauauacgcu acaagaaaag ccaaacuaga caaagauaag     2520

aaagaauaca cuuauacacu agguaaaauc aaagauaucu acgcauuagg aacaaaaacc     2580

ccuucaaaaa caggcuucua uaaauuccua gaucuauaca aaacagacaa aagccaauuu     2640

cuuauguauc aaaaagauag aaaaacaugg gacgaaguua uugagaaaau aaucgaacaa     2700

uaucgcccgu uuaaggaaua cgauaaaaac ggcaaagaag uugacuuuaa ccccuuugaa     2760

aaauacagaa uagagaacgg accuauaaga aaauauuccc gaaaaggaaa cggaagugua     2820

auaaaaucca ugaaauauua ugacaaccua cucgguaaau ucguagacau uacuccaucg     2880

gaaagcaaaa accccguggc acuuuuauca uuaaauccau ggaggacaga uguauacuau     2940

aauccagaaa ccagaaaaua cgaguuucua ggacuuaaau acgcagauuu auguuuuggu     3000

aaaggaggcu cuuacgguau aucagaagua aaauacaaca aaauaagaga gaaagagggg     3060

aucggcaaag aaucagaauu uaaauuuaca cuauauaaaa acgaucuaau acuaauaaaa     3120

gauacagaaa caaacuguca gcaauuuuuc agauuuuggu ccagaacagg aaaagauaac     3180

cccaagagcu uugaaaaaca uaaaauagaa uuaaaaccuu acgaaaaagc aaaauuugaa     3240

aaaggagaag aacuagaagu auugggcaaa guaccgccau caucuaacca auuccagaaa     3300

aacaugcaaa uagaaaaccu cucgauauau aaaguaaaaa ccgauguacu cggcaauaaa     3360

cacuuuauca agaaagaagg agacgaacca aaacuaaaau uuaagaaaug a              3411


<210>  5159
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5159
augaauggcc uugucuuagg uuuagacaua ggcaucgcaa guguaggggu cgggauacug       60

aaaaaagaca cgggagaaau aauacauacu aauucucgac uuuucagcgc ugccacagcu      120

gauucaaaca uagaaagaag acgacaucgc ggcgggaaga gacuuacaag acgaaaaaag      180

cacagaucua uuagauuaca ugauuuguuc gaagauucug gauuauuaac ugauuuuagc      240

aaaguaucaa uuaacuugaa uccauaccaa cuucgaguuc aaggcuuaga caaccaacuu      300

accaaugaag aacuauucau agcucucaaa aacauaguaa aaagacgagg uauaucauac      360

cucgacgaug cuucugaaga cggcggcaca guaucaucag auuacggcaa agcagucgaa      420

gaaaauagaa aacuccucgc ugaacaaaca ccgggacaaa uacaauuaga cagguuugaa      480

aaguacggac aaguaagagg cgacuucaca guuguagaaa acggcgaaaa aagacgacua      540

auuaacguau ucacaacuuc agcauauagc aaagaagcag aaaggauauu gcggaaacaa      600

caagaauuca auaagaaaau aacagacgaa uucaucgaaa auuauuuaac uaucuuaacg      660

gguaaaagaa aauauuacca cggaccuggc aacgaaaagu ccagaacaga uuacggcagg      720

uauacaacaa agaaagaccc ugaaggaaag uacauaaccc uagacaacau auuugguaua      780

cucauaggca aauguacguu cuacccggac gaauaucgcg cgaguaaagc aagcuauacu      840

gcacaagaau uuaaucuauu aaacgauuug aauaauuuaa ccguacccac ggaaacaaaa      900

aaauugagug aagaacaaaa aaaaacaaua auaaaauacg cgaaaacagc aaaaacacua      960

ggagcuucua cacuacuaaa auauauagcc aaguuaauag gagcauccgu agaucagauc     1020

cauggauacc gaauagaucc caacaaaaag ccugaaaugc auaccuuuga aacuuauaga     1080

aaaaugcaaa guuuagaaac aauuagugua gaagaaauac caagaaaagu cuuggaugag     1140

uuagcacaca uauuaacccu gaacacagaa agagaaggaa uagaagaagc aauaaaugcc     1200

acccuaaaag acacauucag ucaagaccaa guacuagaau uaguccaauu cagaaaaaau     1260

aacucaucau uauuuuccaa aggauggcac aacuuuucuu uaaaguuaau gauggaguua     1320

auaccagaac ucuacgaaac aucagaagaa caaaugacca uccucacgcg acucggaaaa     1380

caaaaaagca aagaaacaag uaaaagaaca aaguacauug acgaaaaaga auuaacagaa     1440

gaaauuuaca acccuguagu agccaagagu guccgacaag cgauaaagau uaucaaugaa     1500

gcaaccaaaa aauaugggau auuugauaau auaguaauag agauggcaag ggaaaacaau     1560

gaagaagacg caaaaaaggg auacaacgaa aggcagaaag ccaaucucga cgaaaaaaaa     1620

ucggcgaugg aaaaagccgc auuucaauac aaugguaaaa aagaacuacc agacaacguu     1680

uuccacggac acaaagaacu agcaacuaaa auaagguuau ggcaccaaca aggagaaaaa     1740

ugcuuauaua ccggaaaaaa cauaccaauu ucggacuuaa uccaaaacca auacaaauau     1800

gaaauagacc acauacuacc auuaaguuua ucuuucgaug acucucucuc aaauaaagua     1860

cuaguacuag ccacagcaaa ucaagaaaaa ggacaaagaa ccccuuucca ggcacuagac     1920

agcauggacg augccuggag uuauagagaa uuuaaaaguu acguaaaaga cuuaaaacua     1980

cuaagcaaua aaaagaaaga cuauuuacua acagaagaag auauaucaaa aauugaagua     2040

aaacaaaaau uuauagaaag aaacuuaguc gacacacgcu acucaaguag aguaguauua     2100

aaugcgcuac aggacuuuua uaaagaacau caauucgaua caacgaucuc aguaguuagg     2160

ggacaauuca cgucacaguu acgaagaaaa uggggacuag aaaaaucaag agaaacauau     2220

caucaucaug ccgucgacgc ucuaauaauc gcugcgucaa gucaacucag acuauggaaa     2280

aaacaaaaca acccacuuau uucauauaag gaaggccaau uuguggauca agagacaggu     2340

gaaauaauau cacuaagcga cgacgaauau aaagaacuag uauucaaagc accguacgac     2400

cauuucguag acacucucaa aucaaaaaaa uucgaggauu caauccuauu cuccuaucaa     2460

guagauucua aauacaaucg caaaauaucg gaugccacua uauacgcaac acguaaagcu     2520

aaacucgaua aagaaaaaaa agaauacaca uauacacucg gcaaaauaaa agauauauac     2580

gcacuggguu cagaaaaacu caaaacggga uuuuacaaau uccuucaucu cuauaaaaag     2640

gacaaaagcc aguuccuaau guaccaaaaa gauagaaaaa cgugggacga agucaucgaa     2700

aagauacuag aacaauauag accguucaaa gaaaaggaca aaaaugguaa agaagucgau     2760

uucaauccuu uugaaaaaua uaggauagaa aacgggccaa uacauaaaua cucaagaaaa     2820

ggcaacggac ccguaauaaa aucccuaaaa uacuacgaca aaaacauccc agagucuuuu     2880

aucaauauua cacccaguaa uucaaaaaau aaaguuauau uaaggcaauu aaaccccugg     2940

agaacagacg uauacuucaa ccaucaaaca gaaaaauaug aacuauuagg cuuaaaauac     3000

gcccacuuac aguucgaaaa aaaaaccgga aauuacagca uaacaauaga uaaauacaau     3060

gaaaucaagg aaaaagaagg agucucagaa cagucagaau ucaaauucac acuucauaaa     3120

aacgaucuau uauuaauaaa agacaccgaa acaaaagaac aacaacuauu ccguuuccua     3180

uccagaacaa aaccuaacgu aaaacacuau guagagcuaa agccauauag uagagacgaa     3240

uuugaaaaaa acgaaagauu gaucgaaauc cucggcagcg uagauaaaag cggaagauuc     3300

guaaaaggau uagggaaauc aaaucuaagu auauacaaag uaagaacaga cguauuagga     3360

aaccaacaca uaauaaaaaa agaaggagaa gaaccaaaac uuaaauuuug a              3411


<210>  5160
<211>  3411
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5160
augaauggac uaguacuugg auuagauaua ggaauagcuu caguaggagu aggcauauua       60

gaaaaagaca caggaaaaau uauccacgcc aguuccagac uauucccggc ugcaacagcc      120

gacaauaaug ucgaaagaag aucaaaccga caaggccgua gacuaaaucg caggaaaaaa      180

caucgaucag uaaggcuuca agauuuauuu gaaggauacg gacuauuaac agacuuuagc      240

aaaguaagua ugaaucugaa uccuuaccaa uuaaggguac aagggaugga aaaccaacuc      300

acaaacgaag aguuauucgu ugcacugaaa aauauuguca agagacgagg gauaucauac      360

uuagaugaug caagcgaaga uggagggaca guauccucug acuacggaaa agccguagaa      420

gaaaaucgca aacuucuugc agaaaaaaca ccgggacaaa uacaauuaga aagauucgaa      480

aaauacgguc aauuaagagg agauuucacu guagaagaaa auggagaaaa acaucgacua      540

auuaaugucu ucucaacauc ugcauacaga aaagaagcag aaagaauccu uagaaaacaa      600

caagaauuua auuccaaaau cacggaugag uuuauagaag acuaucuaau uauauuaaca      660

ggaaaaagaa aauacuauca ugggcccggc aaugaaaaau caagaacaga cuauggaaga      720

uucaggacag acggaacuac cuuagacaac auuuuuggua uacuaaucgg caaauguaca      780

uucuauaccg aagaauauag agcaaguaaa gcaaguuaua ccgcucaaga auucaaucua      840

cucaaugacc uaaauaacuu gacaguccca acugaaacga aaaaacucuc agaagaacaa      900

aaaaaauuga uuauagaaua ugcaaaaucu gcaaaaacac uaggugcaag uacucuccuc      960

aaguauauug cuaaaaugau agaugcuuca gucgaccaaa uacgcggaua ccgaguagac     1020

guaaacaaca aaccagagau gcacacguuc gagguauaca ggaaaaugca aagccucgaa     1080

acaauaaaag uagaagaauu accgaggaaa guuuuagacg aacuagcgca cauacuaacu     1140

uuaaacacag aaagagaagg aauagaagaa gcaaucaaua gcaaacuaaa agacauauuu     1200

aacagagacc aaguacuuga gcucguacag uucagaaaaa auaauaguuc ucuauuuuca     1260

aaaggauggc acaauuucuc uaucaaacuu augauggaac uaauacccga auuguacgaa     1320

accagcgaag aacaaaugac aauacucaca agauugggca aacaacgcag uaaagaaaca     1380

ucaaaaagaa cgaaauacau agacgaaaaa gaauuaacug aagaaauaua uaacccaguu     1440

guugcaaaau caguaagaca agcaauaaaa aucauaaacg aggcuaccaa aaaauacggc     1500

auuuucgaca acaucguuau agaaauggcc cgagaaaaca augaagaaga cgcaaaaaaa     1560

gacuauauca aacgacaaaa ggccaaccaa gacgaaaaaa augccgccau ggaaaaagcg     1620

gcauuucagu acaauggcaa gaaagagcua ccagauaaua uuuuccaugg ucauaaagaa     1680

cucacaacaa agauccgacu uuggcaucaa cagggagaga aaugcuuaua uacugggaaa     1740

aacauaccaa ucuccgaccu aauccauaac caauacaaau augaaauaga ucauauacuc     1800

ccauuaaguu uaaguuucga cgacaguuua ucuaacaaag uacuaguacu ggcaacugca     1860

aaccaagaaa aaggacaacg uacaccuuuc caagcccucg auucaaugga cgacgcaugg     1920

ucuuacaggg aauuuaaauc uuacgugaaa gauucaaagu uacucagcaa caaaaaaaaa     1980

gauuaucuau uaacagagga agauauaagc aaaaucgaag uaaagcaaaa auucauagaa     2040

agaaacuuag uagacaccag auacucauca aggguaguuc ucaacgcacu gcaagacuuu     2100

uauaaaagcc aucaauuaga cacaacuauu ucagucguua gaggacaauu cacgagucaa     2160

cuacgcagaa aauggggaau cgaaaaaagu agagaaacgu aucaccacca cgcgguugac     2220

gcacuuauca uugcggcaag uucucaauua cgauuaugga aaaaacacuc caauccacua     2280

auagcauaua aagaaggaca auuuguagac ucagagacag gagaaauagu aucacuauca     2340

gacgaagaau acaaagaacu cguuuucaaa gcgccauacg accauuuugu agacacucua     2400

agaucuaaaa aauucgaaga caguauauua uucucuuacc aaguagacag caaauauaac     2460

cgaaagauau cugaugcuac uauuuaugca acgaggaaag cgaagcuuga uaaagaaaaa     2520

aaagaauaca cuuacacacu aggaaaaaua aaagacauau acgcccucgg uacaaagaca     2580

cccuccaaaa cuggcuucua caaauuucuc gaccuauaca aaacagauaa aagccaauuc     2640

cuaauguacc aaaaagacag aaagacaugg gaugaaguaa uagaaaagau aauagaacaa     2700

uacagaccuu ucaaagaaua ugacaaaaau ggcaaagaag ucgacuuuaa uccauuugaa     2760

aaauacagaa ucggaaacgg uccaauacgc aaauacucua aaaaagggaa cgguccagaa     2820

auaaaaucac uuaaauacua ugacauacua cuaggaaaac auaaaaauau aacaccugau     2880

ggcucgcgaa auaccguagc auuacuuuca cucaauccuu ggcgaacgga ugucuacuac     2940

aacucagaaa cuaaaaaaua cgaauuucua ggacuuaaau augcugacuu augcuuugaa     3000

gaagguggag cauauggaau aucagaagua aaauauaaaa aaauuagaga aaaagaaggc     3060

auaggaaaaa auucugaauu uaaauucaca cuauauaaaa acgaccucau acuuauaaaa     3120

gauacggaaa caaacuguca acaauucuuu agauucugga gcagaacagg gaaagacaau     3180

cccaaauccu uugaaaaaca uaaaauugaa uuaaaacccu acgaaaaagc caaauuugaa     3240

aaaggagaag aacucaaagu acugggcaaa guuccgccau ccagcaacca auuucaaaaa     3300

aacaugcaaa uagaaaauuu gucaauauau aagguuaaaa ccgacaucuu aggaaacaaa     3360

cauuuuauca aaaaagaggg agaugaaccc aaacuuaaau ucaaaaaaug a              3411


<210>  5161
<211>  3420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5161
augucagacc uuguucuagg ccucgauauc ggaaucggau cuguaggcgu aggaauauua       60

aauaaaguua ccggggaaau aauccauaaa aauaguagaa uauucccagc ugcacaggcc      120

gagaacaacu uagaaagaag aacuaauaga caaggcagga gauuaacacg aagaaaaaaa      180

caucgacggg uuagacucaa ucauuuauuu gaggaaucag guuuaauaac agacuucaca      240

aaaguaucga uaaacuuaaa ccccuaucaa uuaagaguaa aaggauuaac agcagaauua      300

aguaacgaag aauuauucau ugccuuaaaa aacaugguua aacaccgagg aaucucauac      360

cuagaugacg caagcgacga cggaaauagc uccguagggg auuaugcgca aauaguaaaa      420

gaaaacucaa aacaacuaga aaccaaaacg cccgggcaaa uccaacuaga acgauaucag      480

aaauaugguc aauugagagg agauuucaca guagaagaag acggcagaaa acacagacua      540

auaaacguuu uuccaacauc ugcguaccac gcagaagcac uacgcauccu acaaacucag      600

caggaauuca acccacaaau aacggacgaa uucaucaauu cuuaccuaga aauauuaacg      660

gggaaaagaa aauauuacca ugguccagga aacgagaaau cacgcacaga uuacggaaaa      720

uauacaacaa aaaaagaugc gcaaggacaa uauauuacac uaaacaacau auucgguauc      780

cuaauaggua aauguacguu uuauccagaa gaauacaggg cagcaaaagc aaguuauaca      840

gcacaagaau uuaauuuacu uaacgaccua aauaauuuaa caguaccaac agaaacaaag      900

aaacuuucag aagaacaaaa guaccaaaua auaacauacg ucaaaaacga aaaagcaaug      960

ggacccgcaa aacucuucaa auauauagca aaauuauuau caugugaugu agcugauauc     1020

aaaggauacc gaauagauaa auccgacaaa gcggaaauac acacauuuga agcuuaccgg     1080

aaaaugaaaa cuuuggaaac ccuagacguc aaaaaaaugg cccgagaaga auuggauaaa     1140

uuagcauacg uacuaacucu aaauacagaa agagaaggca uacaagaagc ccuugaccau     1200

gaauuugcag augguaccuu cucacaagaa caagucgaug aacuggucca auucagaaaa     1260

gcuaacucuu caauauuugg gaaaggaugg cauucauuua guguuaagcu aaugauggag     1320

cuaauaccug aacuauacgc aacuagugaa gaacaaauga ccauacuaac cagacuagga     1380

aaacaaaaga ccacuaguuc cucaaacaaa acaaaauaca uagacgaaaa acaauuaacu     1440

gaggaaauau auaaccccgu aguagccaaa agugugaggc aggcaaucaa aauagucaac     1500

gcagcaauua aaaaauaugg agauuuugau aacauaguaa uugagauggc uagagaaaca     1560

aaugaagaug acgaaaaaaa ggcaauacaa aaaauacaaa aagcgaauaa agcagagaaa     1620

gaugcagcaa ugagaaaagc cgccaaccaa uacaacggca aagcagaauu accccacuca     1680

guauuucaug ggcauaaaca auuagcaaca aaaauucguu uauggcauca acaaggugaa     1740

cgaugccuuu auacaggaaa aaccaucuca auacacgacc uaauaaacaa cccaaaucaa     1800

uucgagauag accacauuuu gccacuaagu auaacauucg acgauagcuu agcuaauaaa     1860

guccuaguau acgccaccgc aaaucaggaa aaaggacaaa gaaccccaua ccaagcauua     1920

gacucuaugg acgaugccug gagcuuccgg gaacugaaag cguucguaag agaaaguaaa     1980

gcuuugagua acaaaaaaaa agaauaucua cuaacggaag aagauaucuc aaaauucgac     2040

guuagaaaaa aauuuaucga aaggaacuua guagauacaa gauaugcuuc acgcgucgua     2100

cuaaacgcac uacaagaaca cuuuagaacu cauaaaauag acacaaaagu aaguguagua     2160

cgaggacaau ucacaucaca acuacgaagg cauuggggaa uagaaaaaac cagagacaca     2220

uaccaucauc augcgguaga ugcacuaauc auagcagcua gcucccaacu aaaccuuugg     2280

aaaaagcaaa aaaacaccuu aguuucauau ucugaagacc aacuccuuga caucgaaaca     2340

ggagaacuga ucucagacga ugaauauaaa gaaagcguau uuaaagcccc auaccaacac     2400

uucguagaca cgcuaaaauc aaaagaauuu gaagacucaa uccuauuuag cuaccaagua     2460

gacagcaaau ucaaucgaaa aauaagcgau gcaacaauau acgcaacaag gaaagcuaaa     2520

cuagauaaag aaaaaaaaga auauacguac acguuaggaa aaauaaaaga caucuacgcu     2580

uuagggacaa aaacaccauc aaaaaccgga uuuuauaagu uucuagaucu auacaaaaaa     2640

gacaaaucac aauuuuuaau guaccaaaaa gacaggcgaa caugggauga aguaauagaa     2700

aaaaucuuag agcaauacag accuuuuaaa gaaaaagaca aaaauggaaa agaaguugau     2760

uuuaacccuu ucgaaaagua uagaauagaa aacgguccua uccggaaaua uuccagaaaa     2820

ggcaacgggc cugagauaaa aucccugaaa uauuaugaua accuacuagg aagauuugua     2880

gacauaacuc cuagcgaaag uaaaaaccca gucgcgcuac uaucuuuaaa cccauggcga     2940

acugauguau acuauaauac agaaaccaga aaguacgaau uccugggcuu aaaauacgcc     3000

gaccuauguu uugagaaagg uggaucauac ggaauaucua aaguuaaaua caauaaaaua     3060

cgagaaaagg aagguauagg uaaaaauagu gaguuuaagu uuacucuuua caaaaaugac     3120

uuaauauuaa uaaaagauac agaaacaaac agacagcaaa uauuuagauu uuggucaaga     3180

acaggaaaag acaacccaaa auccuucgaa aaacauaaac uagaacucaa accauaugaa     3240

aaaaccagau ucgaaaaagg ugaagaacua aaaguucuag gaaaaguccc gccaucaagc     3300

aacagauuac agaaaaauau gcagauagaa aauuuaucca uauacaaagu cagaacagau     3360

guacuaggaa accaacacau cauuaaaaac gagggugaua aaccaaaacu cgacuuuuga     3420


<210>  5162
<211>  3420
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5162
augaacuauu auaauggauu cauaauaacc gaaaaugcaa uggccucgaa acacgaaaau       60

uugggcgcau uaacauuugg auuagacauu ggaauagcua guguagguug ggcgguacua      120

ggcgaaaauc gaauaauaga uuuaggagua cgcuguuuug auaaagcaga aacugcaaaa      180

gaaggugaau ccuugaacuu agcgagaagg acagcgaggu uauuaagaag acgacuuagg      240

agacgagcau ggagacuuac aaaacuagca agacuacuaa aacgagaagg auuaauagca      300

gacacaaaug uuuuucaaaa accauucaac uugaaagaug caaacgaaaa aggucuaagu      360

uucuggcaau uaagaguaga cgcuuuagac agaaaauuaa gugcagaaga augggcgaga      420

guaaucuacc accuaugcaa acaccgugga uuccauugga ucagccgagc ugaagaaaaa      480

gcagcagagg gagauuccaa gagcgaaggu ggaaaaguaa aacaaggauu ggcaggaacu      540

aagaaacuaa ugcaagagaa aaauuaccga acugccgcag aaaugguccu cacagaauuu      600

ccagaagccc aaagaaauaa acaaggagac uauagcaaag cacuauccag aauacuacua      660

ggagacgaaa ugacucaauu auucaagcau caacgagaac uuggaaaccc acacgcaaca      720

gaaaaguuag aagcagccau acuaggaaau ggagaccaca aaaguggacu auuuugggcc      780

caaaacccag cauuagccgg aaaugauuua cuaaaaaugc uaggacauug cacauuugaa      840

aaagccgaau auagaguccc aaaagcaucu uuuacagcug aaaggcaugu uugguuaacu      900

aggcuaaaua auuuaagaau ugucguagac ggaacaacua gaccauuaaa cgaagcugaa      960

aggcaucugg cccuuuuaau gccauaucaa caagccggug auuuaacaua uaagcaauua     1020

cgaacagcau uaaccaaagc aggguauuua ccagaaaguu uucgauauac aggucuagca     1080

uaugcaucgg gucaaaaagc ggaugaaaaa gcuaaagacc ccgaaucagc accuuuagua     1140

aaacuuccgg ccuggcauga auugagaaaa accuuggaaa agaaagaauu agccuccgaa     1200

uggcagaaaa uuucauccga ugcacuugau ggaaaaccac aauuacucga ucaaaucgcc     1260

uggguauuaa gcguauacaa agacgauacc gaaguugagg cagaacuaug caaauuaaau     1320

cuaccaaaug cagagaaaau gauagaugca cuucuagaua uacguuuuga caaauuuagu     1380

aaucugucgc uaaaagcacu gagacaaauu guaccacuaa uggaacaagg ucaaagauac     1440

gacgaagcgg uggcuaacau uccagaauau ggccaucacu cacaauuaca ugaaguuggc     1500

gcgggugaac auaaauacuu accaccauuu uauaaagaac gagauaaaac agguaggaug     1560

guauucaaug acgauaugga uaucccacga aacccuguug ucuuaagagc auuaaaccaa     1620

gcuagaaaag uaguuaacgc ucuaauuaaa gaauauggga guccacacga aguacacaua     1680

gaaauggcga gagauuuaag uagaccacuu gaugaaaggc gugaugucga aaaauuacaa     1740

aaagaguucc gcgaucgaaa ugaaaaagac aaggcuuuau ucgcacaaga uuuuaauaua     1800

guaggagcag uuaaaggcaa agaauuugaa aaauaucaau uauauaggga acaacaaggg     1860

aaaugugcgu auucuauaga accaauggau auucacaggu uauucgaaca aggauacgua     1920

gaaauugauc augcacuacc auauaguaga ucauucgaug acagcaaaaa caacagagua     1980

uuaguccuag caaaagaaaa ucgugauaag ggaaacagaa caccauuuga auauuuaggg     2040

ggcaaggaca acucagaaag guggcggcag uuuguagcau uuguagaagg uaauaaauca     2100

uacagacuag caaaacgauc uagguugcua agaaaagacu uuggaaagga ugaagcaaaa     2160

ggcuuuagag aaagaaauuu aaacgauaca agguauauau guagauucuu uaaaaauuac     2220

guagaacaau auuugcaacu ucaugaagac agugacgcua aacguugcgu agucuuauca     2280

ggacaaauga cuagcuuuuu acgagcacgu uggggauuag uaaagguccg aggagauuca     2340

gacagacauc acgcccucga uggagcaaua guugcagcau guucacauuc aaugguuaaa     2400

cggcuuagug acuacucaag aagaaaagaa uuagaacaag uaagggcagg uuuuguagau     2460

guagacacgg gagaaauugu uaauccagcc auguuucaac aaguacaaga gcauuuccca     2520

aauccuuggc cacauuucag agaugaaguc aaauuaagau uaaacguaga ugacccagcu     2580

cuguuacacu cagaaaugaa uagauuuggu acuuauucac cagaagcauu gaaggcuuua     2640

cgaccucuuu uuauaaguag agcaccacaa agaagaaaua guggcgcagc acacaaagau     2700

accauauacg cacaaccaga aagauuaguu gcucaaggug guguaacaca aaaaguacca     2760

cuaaccaguu uaacgcuaaa agauuuagac aaacuuaucg auccacauag aaaugaaaaa     2820

uuauauucag caauaagaau aaggcuagaa caacauggag gaaaaggaga uaaagcauuc     2880

ccagcuucaa auccauuaag aaagccagau ucggaaggca accccacagg ccccauagua     2940

aggggcguaa caauggucau cgauaagcuc aguggaaucc caauacgggg cgggauagcg     3000

aaaaacgaua guaugguccg ugucgacguu uucaccaaag caggcaaauu ccaucuagua     3060

ccaguauacg uucaucacgc aguagcgaaa guauugccaa auagggcugu gguugcauau     3120

aaagaugaaa cagaauggac uuuaauagac gauggauucg auuuuuguuu uucaauguac     3180

ccuaaugacc uuuuaagaau aaaguugcgu aaugcaagcc aucuugguua uuaugcuucu     3240

ugcaauagga gcacaggugc aauuaacuua uggagucaug acagaaauca acagguuggc     3300

aaagacggga ugauagaauc aauagggguc aagacagcac uaaauguuga aaaacuaaau     3360

auagacguac uagggaauag guauccugca ccacccgaaa aacgaagaga uuuagcuuga     3420


<210>  5163
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5163
augaaaaaca ugacaaaagu aaacgucggu cuagacguag gaaucucuuc ugcaggcugg       60

ucagucauca augcagaaac aaaccaaaua cuugaauccg gaguccgacu uuucagcgga      120

ucagacgcag acgagaaugu caacagaaga aaccaacgac aaggacggag acuauucaga      180

agaagaaaac uaagaauaca agaagcaaag aaauaccuug aaaacgucuu ucaacuaaaa      240

gauauagaac caaaagacga auggaaccca uacgaaauaa gaguaagagc uuuaaaugaa      300

aaaguaagca aagaggaauu gagcaucgcc cuuuuaaaua uaguuaaaca uagaggaaua      360

ucauaugaac ucgaagacgu aacagaugaa gaaggaacaa auacacaauc auuccaauca      420

ggauuagauu auaacagaag acuccugaaa acaaaaacag uaggagaaau acaauaugac      480

cgucuccaga aauaugguca agugagagga caaauacaga cagaggaugg ucuacuaaua      540

aacaucuucc ccacuaaaaa cuacguagaa gaaguagaaa gaauauuaga aacacaaaag      600

aaauaccacc ccgaaauaga cgacguguuc auagagaaaa uuaucagcuu cguaacacga      660

aaaagagaau auuucgucgg accgggaaac gagaaaagca gaacagacua cggaaucuac      720

cgggaaaacg gugaaacacu agauaaccuu uuugaaauau ugauaggcaa agauaaauuc      780

uacccagaag aauacagagc agcagguuca ucguauacag cacaaauauu uaaucuuuua      840

aacgaccuga acaaccuaag aauaccuaca acagaagauc aucaccucac uaaagcccaa      900

aaggaagaaa uuauaaccga acucaaaaac acaucaagac uaucaggagg aaugcuaaaa      960

uuaaucucca aggucacaaa agccccaaaa gaagaaauau cuggauacag aaaagacgaa     1020

aaagauaaac cagaucucca uucacuagcc uauuaucgaa aacagaaaaa uauguuagaa     1080

aaagaaggca uccaaauagc agauuuuagu gaagaauugu uagacgaacu ugcaacaaug     1140

augacauuaa acacagaacc gggggaaaua agaaaacaau uaaacaaaau aacaaaccaa     1200

uacgacauac uuacaccaga aguuauagaa guucuaguca aaaaucauac ggcauuugua     1260

gcaacaucua aucauaaaug gcauagauuu ucucuaaaau uaaugaaaga guucauucca     1320

gaacuacuag aaacgagcaa agaacaaucc accauccuau gcgagagagg auucauacaa     1380

aauagagcaa guuauuuaga ugaaaaucau cacuuuaauu acgcagaauu acgcgagaau     1440

cuauauaacc caguaguaag aaaaucaaca ugugaaaugu ugaaaguauu cacagccaua     1500

caggaaaaau accccaacau ucaaaaugua auaguagagc uuccucgcga aaccaacgaa     1560

gaugaacaaa agagaaaaau aaagaaaaua aacgacaaca augcaaaacg aaagaaagag     1620

auuuuaaaag aagcccaagc acaaucagga cucucugaag aagcucuuga agaaaaacug     1680

uugaaaacaa aaggacuaua uuuaaaacuc augcuuuggu augaacaaga aggcaaaugc     1740

gucuauucag gacaagagau accguuaucu gaccuacugc aaaauaaagg uggauaugaa     1800

guagaccaca uuauacccau aaguauauca uucgacgaua gguuauguaa caaaguucua     1860

guaagacaac aacuaaacuc ucagaaaagc aaccaaacac ccuucgaaua uaugacaaca     1920

cacacagaug gacaccuauc uuuccacgaa uuaaaagaau uagucgguaa aaacaaaaga     1980

uucucaaaag agaaaaggga acuacuccua gcagaaauau acucaaacga caucgaaaca     2040

cgaaaacgau ucauaaacag gaaccuagug gauacaucau acacagcaag gaucguauug     2100

aacgcccuuc aacaauacaa gaaagaaaac caacuaagca ugaacguuag cguuguacga     2160

ggagcaauga caaacauucu acgaaagaag guaaagcuag acaaaacaag ggagacauuc     2220

caccaccacg cgauagacgc gagccuaaug gcagucauac caaacauuga guucuucaaa     2280

gaacgaaaag aaugguuacc aaaaagcgca aaugaagaag uaguagaagu agaaguuucu     2340

gaagaagaaa ccaugcccga aaaagaauuu aaaguaaaaa ugagauuacc ggaaaguuuc     2400

auccaagaug ugcaaauacu acaacgaaac agcuucgcaa aagaagaagu aagcggacca     2460

caaacaagag aacgaaaccu uaaaauaaaa uucagccacc aaguagacca gaaaaugaac     2520

aggaaaguug cggacgacac aauuuaugga acgcgucaag cgcaacuagg aaaagacaag     2580

aaagaagaaa cauacguuau aggaaaaauu aaagacauau acaacuacga ugaauaugua     2640

agauucaaga aacgauauga caaaaacaaa gagagcuucc uaauguauca ccaugaccca     2700

cagacauuug agaaacuaga agaaauucua gcucaauacc cugaauauau agaagacgaa     2760

aaaggaaaga aaaagaaagu aucuccauuc gcucuauauc gagaagaaaa cggcccagua     2820

acaaaauacg caaagaaaaa uaacggacca guaaucaaac aguuaaaaua cuaugacagc     2880

aaacuuaacc gcaaaauaga uauaucacac aaauauagca ccaaaaaccg acaaguggua     2940

cuccaaagcc ucaaaccuua uagaacagau guauacagaa aucaagauac aggagaauau     3000

acacuggugg gaauugacca gaaugauggu aaauuuguaa acggacacua cgguauaccc     3060

aaaaguagau augaagaaau aaaagccaac aauaaauuaa gcgaacacga cgaauuugua     3120

uucucccuau auaaaaacuc uaugguaaaa guuauagaug aaaacggcga aagcauagua     3180

guuagauacc uaucaagaag uaugccaaac cguaaaaaca acgcagagau uaaaccggug     3240

gaaaaauacc aauucgaaga uggagaggua guaccuguau acgguaaaug cagaaaacaa     3300

augcaaacag uauuugucaa aaagggcugg acguuacuaa aaggaaacac agacauucua     3360

ggaaauaccu acuucguaga aaaagaaagu gagaacccaa agaaaaugau ugaugauaaa     3420

uga                                                                   3423


<210>  5164
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5164
augagagaaa acggaaguga cgaaagaaga aggaauaugg augagaaaau ggacuauagg       60

aucggacuag acauagggau agcaaguguc gguugggcug uauuacaaaa uaacagugac      120

gaugaaccag ugagaaucgu agaccugggc guaagaauau uugauaccgc agaaauaccu      180

aaaacaggcg aaucauuagc uggaccacgg agagcagcca gaacgacuag acguagauua      240

agacgccgua aacacagacu ugaccggaua aaaugguuau ucgaaaacca aggacuaaua      300

aacaucgaug acuuuuuaaa aagauauaac auggcaggcu uaccagaugu auaccaauua      360

agauaugaag ccuuagauag aaaauugaca gaugaagaac uggcacaagu acuacuacau      420

auagcaaaac acaggggauu ucgcaguacg aggaaagcag aaaccgcagc aaaagaaaau      480

ggcgcaguac ucaaagcaac cgacgaaaac cagaaaagaa ugcaagaaaa agguuaccga      540

acgguugggg aaaugauaua ccucgacgaa gcauuucgaa cagguugcuc auggagcgaa      600

aaggguuaua uacugacacc cagaaacaaa gccgaaaauu aucagcacac gaugcuaaga      660

gcuauguuag ucgaagaagu aaaagaaauu uuuaguagcc aaagacgucu aggcaaugaa      720

aaagcuacag aagaacuaga agaaaaauac cucgaaauaa ugacaagcca acggucauuu      780

gaucuaggac caggaaugca accagauggc aagccuagcc cuuaugcaau ggaaggauuu      840

ucagauagag ucggaaaaug cacauuccuu ggagaucaag gggaacuuag aggagcaaag      900

ggaacuuaua cugcagaaua uuucguagca cuacagaaaa uaaaccacac uaagcuagua      960

aaucaagacg gcgaaacuag aaauuuuaca gaagaagaaa ggcgggcauu gacucuauua     1020

cucuuuacuc aaaaagaagu aaaauaugca gcaguuagaa agaagcuggg auugccggaa     1080

gauauauuau ucuacaaucu uaacuauaag aaagcagcaa caaaagagga gcaacaaaaa     1140

gaaaaccaaa auacagaaaa agcaaaauuu auagguaugc cuuacuauca cgauuauaag     1200

aaaugucucg aagaaagagu aaaauauuua acagagaacg agguacguga uuuauucgac     1260

gaaauuggca ugauuuuaac auguuacaaa aaugaugaca guagaacaga gaggcuugcg     1320

aaauuagguc uaguaccaau agaaauggag ggacugcuug cauauacgcc uacaaaguuc     1380

caacauuuau caaugaaagc caugcgaaau auaaucccau uuuuggaaaa gggaaugaca     1440

uacgacaagg cuugcgagga agcuggauau gacuuuaaag cagacuccaa aggaacaaaa     1500

cagaaauuau uaacaggaga aaacgucaau caaacaauaa augaaauaac uaauccugua     1560

guuaaacgca gugugaguca aacaguaaaa gugauaaacg caauaauucg caccuaugga     1620

agcccucaag caauaaacau ugaacuugcc cgagaaauga gcaaaacauu cgaagaacga     1680

agaaagauca agggugacau ggagaaaaga cagaaaaaua augaagaugu caagaaacaa     1740

auucaagaac uaggaaaauu auccccaaca ggacaggaca uucuaaaaua ccguuugugg     1800

caggaacaac agggcauaug cauguacucg ggaaaaacga uaccauuaga agaauuauuu     1860

aaaccagguu acgauauaga ucauauacua ccauacucua uaacauucga cgacucguuu     1920

agaaauaaag uucuaguaac cucgcaagaa aacaggcaaa aaggaaaucg aaccccauau     1980

gaauauaugg gcaaugauga acaaagaugg aaugaauuug aaacgagagu aaaaacaaca     2040

auaagggacu auaagaaaca acaaaaacua cuuaagaaac auuucucaga agaagaaagg     2100

ucagaguuca aagaacgaaa uuugacagau acuaaauaca uaacaacugu aaucuacaau     2160

augauaagac aaaacuugga aauggcgcca cuaaaucguc cagagaaaaa gaaacaagua     2220

agagcaguaa auggugcaau uacagcauac uuaagaaaac gcuggggauu accucagaaa     2280

aauagagaaa cugacaccca ccaugcaaug gacgcaguug uaauagcaug cugcacagac     2340

gguaugauuc agaaaauaag cagguacacc aaaguuagag aacguuguua uucaaaaggu     2400

acugaauuug uugacgcaga aaccggugaa auuuuuagac cagaagacua uuccagagcc     2460

gaaugggacg aaauauuugg uguucacaua ccaaaacccu gggaaacauu ccgggcagaa     2520

uuagacguac gaaugggaga cgaucccaaa ggauuucuag auacacacag cgacguagcc     2580

cuagagcuag acuacccaga auauauuuac gaaaaccuaa gaccuauauu ugugagucgu     2640

augccaaauc auaaaguaac aggagcagcu caugcagaua caauacgauc acccagacau     2700

uuuaaagaug aaggaauagu acuaacaaaa acugcacuaa cagaccuaaa auuggacaaa     2760

gacggagaaa ucgacgggua cuacaaccca caaagcgauc uauuauuaua ugaagcacug     2820

aaaaagcaau uauuauugua cgguaaugac gcaaagaaag cauuugcaca agauuuccac     2880

aaaccuaaag ccgaugguac agaaggaccg gugguacgaa aaguuaaaau acaaaagaaa     2940

caaacgaugg gaguauuugu cgauucuggu aacggaauug cagagaacgg uggcaugguc     3000

agaauagaug uuuuccgagu caauggaaaa uauuacuuug uaccuguaua cacagcagac     3060

guuguuaaga aaguacuacc aaaccgcgcg ucaaccgcuc auaaaccaua uggugaaugg     3120

aaaguuaugg aagacaaaga uuucuuauuu agccuauacu caagagauuu gauacauauu     3180

aaaaguaaga aagauauacc aaucaaaaug guaaacggag gaauggaagg aauaaaagaa     3240

accuaugcuu auuacauagg agcagauauc agugcagcaa acauacaagg uauagcacac     3300

gacagccgau acaaauuuag agguuuagga auacaaucac uggauguuuu agaaaaaugu     3360

caaauagaug uguuaggaca ugucuccguc guucgaagug aaaaacgaau gggauuuuca     3420

uga                                                                   3423


<210>  5165
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5165
augaacggau uaguacuagg acuagacauc ggaauagcau cuguuggagu aggaauacua       60

gagaaaaaca guggaaagau uguacaugcc ucgagcagaa uuuucccugc cgcaaccgca      120

gauaacaacg uugaacgccg aaagaaucga caagcgagac gacugaacag gaggaagaaa      180

caccgcggcg uccgauuuca agaucuauuc gaagacuaug gccuacuuac agacuucucu      240

aaaguuucca uuaacuuaaa cccuuaccga cuacgaguug acggacuuaa ucaacaacua      300

acuaaugaag aacuauucau ugcacuaaag aacauaguua aacguagagg cauuuccuau      360

cuagacgaug ccucagagga cgguggaaca guaucaagug auuauggaaa agccguagaa      420

gaaaacagaa aauuauuagc cgagaaaacc ccaggucaga uacaacuaga aagauucgaa      480

aaauacggac aacuacgagg cgauuucacg guaguggaaa auggagaaaa augucgucug      540

aucaauguau uuagcacauc agcauauaag aaagaagccg aacgcaucuu aagaaaacaa      600

caagauuuua acaaccaaau aacggacgaa uuuaucgaag auuaccuaaa aauacucacu      660

ggaaaaagaa aauauuacca ugggcccggu aaugaaaaau caagaacaga cuacggaaga      720

uacacaacac auaaagacuc aaaaggagaa uacguaacau uagacaauau auucggagua      780

cucauaggua aaugcacauu uuacccaaau gaauaucgag caaguaaagc aucuuacacu      840

gcccaggagu ucaaccuacu aaaugacuua aacaacuuaa ccgucccuac agaaacaaag      900

aaacuauccg aagaacaaaa gaaaaccaua auagaauacg caaaaucugc caaaacuuua      960

ggugcaagca cacuauuaaa auacauagca aaaaugauag acaccagcgu agaccaaaua     1020

cgaggauaua gaguugacgu aaauaacaaa cccgaaauac auacauuuga aguguacaga     1080

aaaaugcaau cacuagagac uauuucagua ggagaacuau cgcggaaugu guuagacgaa     1140

cucgcccaua uacuaacacu aaacaccgaa agagaaggaa uagaagaagc aauaaauacc     1200

aaacuaaaag acuccuucuc ucaagaccaa guacuugaac uugugcaauu caggaaaaac     1260

aauucguccc ucuucucaaa agguuggcac aacuuuagcc uaaaacugau gauggaacua     1320

aucccugaac uauacgaaac aagugaagaa caaaugacaa uacuaaccag gcuagggaaa     1380

caaaaaucaa aagaaacaag caaaagaaca aaauauauug augaaaaaga acucaccgag     1440

gaaauauaca auccaguagu cgccaaaucc guucgacaag cgaucaaaau uauaaaugag     1500

gcuacaaaga aacauggaau uuuugacaau auagucauag aaauggcaag agaaaacaac     1560

gaagaagaug cgaagaaaga cuauauaaaa cgccaaaaag caaaccaaga cgaaaaauac     1620

gcagccaugg agaaagcagc uuuccaauau aacggaaaga aagagcuacc agacaacaua     1680

uuucauggcc acaaagaauu agcaacaaaa auucgacucu ggcaccaaca aggagagaaa     1740

uguuuauaua cuggaaaauc aauaccaaua ucggaucuaa uacacaauca guacaaauac     1800

gaaauagacc auauuuuacc acucucauua aguuucgaug auagccuaag caacaaaguc     1860

cuaguacuag cuacagcuaa ccaagaaaaa gggcagcgua caccauuuca agcacucgac     1920

ucaauggaug augcaugguc guacagagaa uuuaaaagcu auguaaaaga gucaaaacua     1980

uuaggaaaua aaaagaaaga auaucuacuu acagaagaag auauuagcaa aauagaagua     2040

aaacaaaaau uuauagaaag aaaucucgua gauacaagau acucuucacg agucguccuc     2100

aacgcauuac aagacuucua caaaaaccau aacuuugaca cgacaauauc agucguaaga     2160

ggccaauuca caucacaacu cagaagaaaa uggggucugg agaaauccag agaaacuuau     2220

caccaccaug cuguagacgc auuaauaaua gcugcgagcu cucaauuacg guuauggaag     2280

aaacaaaaca acccucugau agcuuauaaa gaaggacaau ucguagacuc acaaaccgga     2340

gaaaucauaa gucucaccga cgaugaauau aaagaacucg ucuuuaaagc accauacgau     2400

cauuucguug auacacuauc cagcaaaaca uuugaagauu caaucuuauu cucauaccaa     2460

guagacagua aauucaauag aaaaauuagc gacgcaacaa uuuaugcuac aagaaaagca     2520

aaacuagaua aagaaaagaa agaauauaca uauacucuag gaaaaauaaa agacauauau     2580

ucacuaggaa caaaaacucc auccaaaacu ggauucuaua aauuccuaga ccuuuauaau     2640

aaagauaaau cacaauuccu aauguuucag aaagauagaa aaacauggga cgaaguaauc     2700

gagaaaauaa uggaacaaua cagacccuuu aaagaauaug auaaagcugg aaaacucgua     2760

gacuucaauc cuuuugaaaa auauagacaa gaaaacggac caauaagaaa auauaguaag     2820

aaaggaaacg gcccugaaau aaaaucauua aaauauuacg acauacuacu agguaaacau     2880

aaaaacauaa cgccugaagg cucaagaaac acaguugcac uacuaagccu aaaucccugg     2940

agaacagaug uauacuauaa uauggaaacc aagaaauacg aguuccuggg uuuaaaauac     3000

gccgaucuac cauucgaaga aggaggggcu uacgggauua gcacagaaac auacaacgag     3060

cuaagagaaa aagaaggcau agggaaaaau ucugaauuua aauucacucu guauaaaaac     3120

gauuuaauac uuauaaaaga cacagaaacc aacugccaac aauuuuuccg auuuugguca     3180

agaacaggaa aagacaaccc caaaucauuc gaaaaacaca aaauagaacu aaaaccauau     3240

gaaaaagcaa aauuugaaaa aggagaggaa cuagaaguau uaggcaaagu accaccaagc     3300

ucaaaccaau uccaaaagaa uaugcagauc gaaaaccuaa gcauuuacaa aguuaaaacu     3360

gacguucuag gaaacaagca cuucauaaag aaagaaggag auaagccuaa acuaaaauuu     3420

uga                                                                   3423


<210>  5166
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5166
augaauggcc uaguauuagg guuagauauu gguauagcuu ccguuggagu cggaauccua       60

aaaaaagaua uaggugaaau aauacacaca aauuccaggc uauuuagugc agccaccgca      120

gacaguaaca ucgagaggag aggucaucgc ggcggaaaaa gacucacaag aaggaagaaa      180

cacaggagca uacgccuuca ugaccuauuc gaagacuucg gauuguuaac cgacuucuca      240

aaaguaagua uuaaccuuaa uccguaccaa cuuagaguac aaggucuaga caaccaguua      300

acaaacgaag aacuauuuau agcucuaaaa aacauuguca aacgaagagg cauaaguuac      360

uuagacgacg caagcgaaga uggagguacc guaucaucag auuacggaaa agcggucgaa      420

gaaaauagaa aauuacuggc ugaacagacc ccuggccaaa uccaacuaga ucgauuugag      480

aaauacggac aaguacgagg ugauuucaac guaguggaaa acggggagaa acgcagacuu      540

auaaacgucu ucacaacgag cgcauauuca aaagaagcag aaagaauacu uagaaaacaa      600

caagaauuca auaaaaaaau aacagacgaa uucauagagg auuaccuaac aauauuaaca      660

ggaaaaagaa aguacuacca ugggccgggu aaugaaaagu caagaacaga uuauggaaga      720

uacacaacaa aaaaagaucc ggaagguaaa uacauaaccu uagauaacau auuuggcaua      780

cuuauaggaa aauguacauu uuaucccgac gaauacagag caaguaaagc aaguuacaca      840

gcacaggaau uuaauuuacu uaaugaccua aacaaucuca caguuccaac agaaaccaaa      900

aaauuaagug aagaacaaaa aaagaccaua auuaaauacg cuaaaacagc caaaacacug      960

ggagcaucaa cccuacuaaa auauauagcg aaauuaauug gagcaagugu agaccaaauc     1020

cauggauaca gaauugaccc uaacaaaaaa ccggaaaugc auaccuuuga aacauauaga     1080

aaaaugcaaa gccuagaaac uauaagugua gaagaacuac caagaaaagu acuagacgaa     1140

cuagcucaca ucuuaacauu aaacaccgaa agagaaggaa uagaagaagc aaucaacgca     1200

acauuaaaag auacguucuc acaagaucaa guauuagaau uaguccaauu ucggaaaaac     1260

aacagcucgu uauucuccaa aggauggcac uccuuuaguc uaaaacuuau gauggaacuu     1320

auaccugaac uauacgaaac aucagaagaa caaaugacaa uccuaacgag auuaggaaaa     1380

caaaaaucaa aagaaacauc aaaaagaaca aaauacauag augaaaaaga acuaacagaa     1440

gaaauuuaua accccguagu ugcgaaaucc guucgacaag caauaaaaau aaucaacgaa     1500

gcaacgaaaa aauacggcau auucgacaau aucguaauag aaauggccag agaaaacaac     1560

gaagaagaug caaaaaaaga auacauuaaa agacaaaaag cuaaccuaga ugaaaaaaac     1620

gccgccaugg aaaaagcagc auuccaauac aaugguaaaa aggaauuacc agacaacgua     1680

uuucacggcc auaaagaacu cgccaccaaa aucagacuau ggcaucaaca aggagaaaaa     1740

uguuuauaua cuggaaaaaa uauaccuauu uccgaccuca uacaaaacca auacaaauac     1800

gaaauagacc acauacuacc cuuaaguuua ucuuuugacg auaguuuguc aaauaaagua     1860

cuagucuuag cuacagcaaa ccaagaaaaa ggacaacgaa cgccauuuca agcauuagau     1920

agcauggacg acgcuugguc auauagagaa uuuaaaaguu acguaaaaga caguaaacuu     1980

cucggcaaua aaaaaaaaga auacuuauua acagaagaag auauaucaaa aauagaagua     2040

aagcagaaau uuauagagag aaaccuagua gauaccagau auagcagucg ggugguauua     2100

aaugcguuac aagacuucua caaagaacau caauuugaua cuacgauaag uguaguacga     2160

ggacaauuca cuagccaauu acguagaaag ugggguuuag aaaaaagcag agaaacauac     2220

caucaccacg cgguagacgc uuugauuauc gcagcaagcu cccaauuacg acuauggaaa     2280

aaacaaaaca acccuuugau cucauacacu gaaggacaau uuguggacca agucaccggg     2340

gaaauaauuu cauuaagcga cgacgaauau aaagaauugg uguucaaagc cccauacgau     2400

cacuucguag auacgcuuaa gaguaaaaaa uuugaagaca gcauuuuauu uucauaccaa     2460

guagauagca aauacaaccg caaaauauca gacgcaacca ucuacgcaac ucgaaaagca     2520

aaauuagaua aagaaaacaa agaauacaca uacaccuuag gcaaaauaaa agacaucuac     2580

gcgcuaggua cuaagucgcc aucgaaaaca ggauuuuaua aauuuuuaga uuuauacaac     2640

aaagauaaaa gucaauucuu gauguuccaa aaagaucgaa aaacuuggga ugaaguaauc     2700

gagaaaauaa uugaacaaua uaggccuuuc aaagaauaug augaaaaugg aaaagaagua     2760

gacuucaacc cauucgagaa auaucgcaua gaaaaugguc caaucagaaa auauagcaaa     2820

aaaggaaacg gaccugaaau aaaaaguuua aaauacuacg auaauuuacu cggcaaauuu     2880

guagauauca cgccgaguga aucgaaaaac ccaguugccc uacucucucu aaauccaugg     2940

aggacugacg uauauuacaa caccgaaaca ucaaaauacg aauuccuagg acuaaaauau     3000

gccgaccuau guuuugagaa aggaggcgcc uauggaauuu cagaaguaaa auauaauaaa     3060

auucgagaaa aagaaggaau cgggaaagaa agugaauuca aauucacacu guauaaaaau     3120

gaucuuauuu uaaucaaaga cacagaaaca aacugccaac aaauauucag auucugguca     3180

agaaccggaa aagacaaccc uaaaucguuc gaaaaacaua aaauugaacu caaaccauac     3240

gaaaaagcaa gauucgaaaa gggagaggaa uuagaaguuu uaggcaaggu accccccucg     3300

ucuaaucaac uccaaaaaaa uaugcagaua gaaaaucucu ccauuuauaa aguaaaaaca     3360

gacguacuug gaaacaagca cuucauaaaa aaagaaggug aagaacccaa acuaaaauuc     3420

uga                                                                   3423


<210>  5167
<211>  3423
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5167
auggaagaaa auuuucagua uagaauugga cuugauauag ggauuaccuc cguaggcugg       60

gccguuuuac aaaacaacuc acaagacgaa ccaguaagaa uaacagauuu aggcguaaga      120

auauucgaug uagcagaaaa ucccaaaaau ggcgaugcgc uagcagcucc acgaagagau      180

gcccgaacua cgagaaggcg acuacgcaga agaagacauc gauuagaaag aauaaaauuc      240

cuuuuacagg agaauggcuu gauugaaaug gauucguuca uggaaaggua uuauaaaggg      300

aaucuuccag acguauacca acuaagauau gaaggauugg acagaaaauu gaaagacgag      360

gaguuagcac aagugcuaau acacauugcu aaacauagag gauuucgaag uacaagaaaa      420

gcagaaacaa aaggaaaaga agggggugca guacuaaaag cuaccacaga gaaucagaaa      480

auaaugcaag aaaaaggcua caggacaguu ggagaaaugu uauaccuaga ugaagcauuc      540

cauacugaau guuuauggaa ugaaaaagga uacguuuuaa cuccaagaaa uaggccagac      600

gacuauaaac auacaauucu acgaagcaug uuaguugaag aaguucaugc aauauucgcu      660

gcacaacgag cacuaggaaa ugaaaaagcu acagaagaau uagaaaacgc uuacguagaa      720

aucaugacaa gccaaagaag uuucgauaug gguccaggau uacaaccaga ugguaagcca      780

aguccauaug caauggaagg cuuuggagac aggguaggga aauguacauu ugaaaaagac      840

gaauauagag caccaaaagc aaccuauacu gcagaacuuu uuguugcacu acagaaaaua      900

aaucacacaa aacuaauaga ugaauuugga acuggaaggu ucuuuaccga agaagagaga      960

aaaacuauaa uagauuuacu cuuaucaggc aaagaacuca aguacggaac aauaagaaag     1020

aaauuaaaua uagauccaag ccuaaaauuu aacucauuaa auuacucagc aaagaaagaa     1080

ggcgagaccg aagaagaaag aguaagggau acugaaaaag cgaaauuugc uuccauguuc     1140

uggacauaug aauacaguaa augccuuaaa gacagaaaug aagagaugac aguaggccaa     1200

aaagcagacc uccuugaucg uauaggagaa auacuaacag cauacaaaaa ugaugauagc     1260

cgaucuaguc gacuagaaga ucuaggauua agucgugagg aaauagacgg cuuauuagaa     1320

cuaucaccag ccaaauauca acgaguauca cuaaaagcaa ugcgaaaaau gcaaccuuau     1380

cuggaagaug gccuaaucua cgauaaagca ugugaagcag caggauauga cuuucgugca     1440

uugaaugaug gcaacaagaa acauuuacua aaaggcgagg aaauuaaugc gaucguaaac     1500

gauauaacaa acccaguagu aaaaagguca guuagucaaa caauuaaagu aauaaacgcu     1560

aucauucaaa aauauggaag uccacaagcu gucaauauag aacuagcaag agaaaugucc     1620

aaaaauuuuc aagaucgaac aaauuuagaa aaagaaauga agaaacgaca acaagaaaac     1680

gaaagagcua aacaacaaau aauagagcua gguaaacaaa auccaaccgg acaagacaua     1740

uuaaaauaca gacuguggaa ugaccaagga ggcuacugcc uuuauucagg aaagaaaauu     1800

ccacuugagg aacuuuuugg aggcggauau gacauagauc acauacuccc cuauucaauu     1860

acauuugaug auaguuauag aaauaaagua cuuguaacag cccaagaaaa ucgacaaaaa     1920

gguaauagaa caccuuauga auauuuugga gcagaugaaa aacauuggga agauuacgaa     1980

gcaagaguua gauuauuagu aagagacuau aagaaacaac aaaaauugcu aaagaaaaau     2040

uuuaccgaag aggaaaggaa agaguucaaa gaaagaaauu uaaacgacac uaaauauaua     2100

acaagaguag uguauaauau gauccgccaa aaucuagaau uagaaccuuu uaaccauccc     2160

gagaaaaaga aacaaguaug ggcaguaaac ggagcaguca caucauauuu aagaaaaaga     2220

uggggacuua ugcaaaaaga uagaaguacg gaucgccauc augcaaugga cgcugucgua     2280

auagcauguu guaccgaugg aaugauacau aaaaucucaa gauauaugca aggaagagaa     2340

cuugcauaca gccggaacuu caaauuuccg gaugaagaaa caggugaaau ccuaaauagg     2400

gacaauuuua caagagaaca augggacgaa aaauuuggag uaaaaguacc guuaccaugg     2460

aacucauuua gagacgaauu agauauuaga cuucucaaug aggauccgaa aaacuuucuu     2520

cuaacacaug cagacgugca aagagaauua gauuacccag gauggaugua uggagaagaa     2580

gaaucaccca ucgaagaagg cagauacaua aauuacauac gcccauuauu uguaagcaga     2640

augccgaacc auaaaguaac aggaagcgca cacgacgcca caaucagguc ggcuagagac     2700

uacgagacuc gaggcguagu aauaaccaaa guaccccuaa cggaucuaaa auugaauaaa     2760

gauaacgaaa uugaagguua cuaugauaaa gacagugauc gacuacuaua ccaagcauua     2820

guucgacaau uacuauuaca uggaaacgac ggaaagaaag cauucgcuga agauuuccau     2880

aaaccaaaag cugacgguac agaaggucca guggugagga aagucaaaau agaaaagaaa     2940

caaacuagcg gaguaauggu cagaggcggg acaggaauug cagcaaaugg cgaaauggua     3000

agaauugaug uauuuaggga aaauggcaaa uauuacuuug ugccuguaua uacugcagau     3060

gucguuagaa aaguucugcc uaauagagcu gcaaugaaug gaaaaauagc gagugauugg     3120

aaaacaaugg aagacaacaa uuuuguuuuu agucuguaua guagagacuu gauucauguu     3180

aaaaggaaga aagcgauucc uacaauucua aguucuggug caacuuucga acaagaagaa     3240

uuuuaugcgu auuuuacagg ugcagacaua agcacagugu cuauagcggg cauaucaaau     3300

gacaacaguu uuaaauucag aggucuuggg auucagggau uagaaauauu ugagaaaugu     3360

caaguagaca uauuaggcaa uauauccguc guuagacaug agaauagaca aggauuucau     3420

uga                                                                   3423


<210>  5168
<211>  3426
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5168
auguacucaa uuggauuaga uauaggaauu gcaaguguug gcuacuccgu aauagagaca       60

aacucaggga gaauaguaga auuaggcgca agaauuuuug aagccagaaa uagcgacaau      120

aacaaagaua gacgagauuu ccgaggaggg agaagacuca uuagacgucg aaagacccga      180

uuagacgacg cuaaaagaua cuuagaugac caaggauuua aaaacgauaa aucguuaaua      240

aauguaugcc cauauagucu aagaguuaaa ggauuaaccg aagaacuuuc aaaaucagaa      300

auccgaaaag uaauucuaca cauauuaaaa aaacguggag ucucauauuu aucaucugaa      360

gacuuagaca caaguagugu ggacaacaaa gacucauuca aacaagcggu aacaaaaaau      420

cuuaggcaac ucgaugauaa aacaccagga caaauacaau augaaagauu acaggaauca      480

ggcagagucc gaacggggau aaaccagaac ggacaauacc aauuaaacgu auuugauguu      540

gcaucauaug caaaagaacu aaaacuaauc uuagaaacac aacaacaaua ccaucaagaa      600

auagacgacc acuuucuaga cuucuuuacu aaagacgggu aucgggaaga ugcaggauug      660

auuuacagaa aaagaccaua uuacgacgga ccaggaaacg ccgcccaaaa cucaucguau      720

ggacguuggg caaacuacca agucgaugga aaaccugccg aaaacauauu cgaucaacua      780

auugguaagg auuucacagg aaaaauaaga gcaucuaacc uagguccaag cgcgcagaaa      840

uuuaaccucu uaaaugaccu aaauaauuua cgaauuacaa caagagaaga ggaaaaacua      900

acuuuugagg aaaaacaaga aauauuagaa guacuagaaa acgauagacc caaaaccuuc      960

ggcccacgaa acauugcaaa aauguugaaa gucgauaugg acgacauaaa aggauggaga     1020

cuuaacuccu cagauaaacc ugaaauccau acaaugaagg aauacaauga uaugagacua     1080

guacuagaag caggaggguu ugauauuaaa gccauagauu uggaccacau agaagagaua     1140

gcgucccuua uaacacuaaa uacggaaaga gaagcauuug aacuaucacu uucaaacgaa     1200

auagacaaca aucuaaaaca auuucuugua gagaauuuua augcuuuaag gaaauguaaa     1260

ccuaacaaca guuggcauuc auucucgaaa gaaaaccuac aaagacuaau cacugagcuc     1320

gaacacacuu ccgaugaaca aaacacaaua cuagaaagac ugggacuaaa auucgaucuc     1380

agaucaaaau acagugauag ggauacacuu ccuauccaug aaguacucga agagauauau     1440

aaucccacag uaucuaaauc cguuagacaa acauuuagag uauuaaaugc acuuguaaaa     1500

aaauacggaa aagaaaacau agauuacguu accaucgaaa ugccaagaga uaaaaacgaa     1560

gaugaucaaa aaaaaacaau cagagaaauu caaaaaguga acaaaaauag aaaagacaaa     1620

agcaaagaau auuuccuaaa gaaaucagga uggagcgaag aaaaauucga agaaaaacuc     1680

agaacaagcc gacguuucgc uuccaaacuc uacuacuucu augagcaaga uggccuaugc     1740

gcuuacaccg gccaacaaau acaaccagaa gacuuauuaa cugaacgugu agaaguggau     1800

cacaucauuc cauuaagugu aagccuagau gauagcauaa acaacaaagu ucuuguaacc     1860

uccgaggcga aucaaaaaaa aggacaaaga aguccuuaug augcauucau gagcggcgac     1920

uucaguaaau acagaacaua cgaugaauac aaggcguggg uacuaucaaa caaauacaaa     1980

aaauauaaaa aggcauuauu auuagaagaa agaaacauau uugauccaga gcuagcaaaa     2040

aaauuuagac agcgaaaccu uaacgacaca cgauaugcuu caagaauagu acucaaugca     2100

guacaaucuu uuuuuuacaa aacagauaca aagaccagag ucguaaacgg agcauucacu     2160

cauacacuca gaaaaaaaug gaaccuagca gauaaagaca gagaaacaca ccaccaucau     2220

gcaauagacg caacuuuagu ugcaguaaca ccguuuguaa aggcagaaag auacgaauac     2280

uauaaagaua gugaaggcaa agccaguaug guggauacag ucacuggaac aauaauccca     2340

uacagugaau acaaaaaaaa aaauuuauac gauagaucua auuauuuacu cauguucggu     2400

aaugaaucca caauaaacca agaaaaagaa gacaaaucag cagaguuuau cagaaaccuc     2460

auuccagcaa aaauuuaccc agaaauaaaa uuuucccacc aagucgacag aaaaucaaau     2520

aggcgacuau cagaugccac aauauauagu acacguucuu acacaguaga aaaaggucga     2580

ggaaaaaaua aaagagaagu aauagaaaga gauauaauag gcaaaauuaa agacauuuau     2640

acugcagaug gauaugcuga auauguaaaa uauaaagacu cacuucucgu aaaggaaauc     2700

gaugaaaaaa cauucgagaa auuagaagcu auaauugaag aauuuucaga uaccguagaa     2760

guaacagauc aacaaggcaa aguaaaaaaa guaucaaaau caccauuucu acaguauugc     2820

gaaaaauacg acguaccagg ugucaccaaa uauucaaaaa aaaacaaugg accaguaaua     2880

agaucucuaa aauaccgaua uaccaagcua cgcucccaua uagauaucuc accaaaaaac     2940

agcaagaaca caguggcgcu auuaacacuu gcacccugga gaaccgacgu cuauuacagu     3000

caagaaacug cauccuauca cuuauuagga uuaaaauaua uugaucuauc auuuagagac     3060

ggcggcuacg gaguaucuag aaagagauau gaagaaauaa aaaagaacga aaggauccca     3120

gaagacggag aauuuaaauu uagcuuguac aagaaggacu acauugaaau cgaauucgac     3180

aaaaaaauau uaccaggccu uuaucaauca agagauguua gugccagaaa caaauucagc     3240

augaaaccaa ucgauaaagc ccaauggaca agcaaggaag auguacucau guucggaaac     3300

gucaaagcuu caggaguauu cgaaaaaaca uuaaaaaaaa acuacgucuu acgaaaguac     3360

cacguugaug uacuagguaa caaguucgaa guaaaggaug agauacuuaa agauauacuc     3420

gaauga                                                                3426


<210>  5169
<211>  3429
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5169
auguauagcg uaggucucga caucgguauc gcgucaugug gauacucaau aaucaacgac       60

guaaacggaa auaucguuga acuaggaacu cuauuauuua aaucacgaaa cucagacaac      120

aaugaagaaa gaagaaccaa cagaggaucc cggagacuca uaaaccgucg aagaaccaga      180

cucgacgaug gaaagaaauu acuagcagaa aaaggauuca aacaagacuc uaaacuaaaa      240

cacguauguc cauacgaguu aagaguaaaa gguuuacacg agaaacucac ccaaggagaa      300

aucuacaaag uaguacucca uauccuaaag aaaagaggua ucucuuaccu agacgaaucg      360

gaugaaaacu cuagcgggau agauggugca aguuacaaag aucaagucaa ucuaaaccua      420

aacuuacaaa aagaauuaac accaggacaa auucaacuca aacgucuaaa agaaaacggc      480

agaauaaaaa gcggaguaac auuugaaaau gguuaccaac ucaaugucuu cagugucucc      540

gcguacgcaa acgagcugaa uaaaauacuu acaaaacaau cagaauauua cucagaaauu      600

gaccaaauuu ggauagaccg auuuacgaaa aacggguacc guaaagaagc uggacucguu      660

uaccgcaaaa ggccauauua cgacggacca ggcaacgaga aaaacaacag cauuuacgga      720

agaugggcca acuuuaaaga agauggacac ccgaaagaaa acauauuuga ucagcucaua      780

ggacuugauu caguagauuc cacacaagua agagcaucug caucaacauu aacagcccaa      840

aaauauaacu uacuaaacga ccuaaacaac cuaacaauua uagaagacga ccgaaaauuu      900

acaacugacg aaaaaguauc gauaaucaac uacuuaauga cccaggauau cacacgauuc      960

ggucccaaag aacuagcaaa auuuuaccag cuagaaguug aucaaauucg ugguuggaga     1020

auagacaaca aaggaaaccc ggaaauccau ucauuaaaag uauacagaga uuggaagaaa     1080

auauucaaca caaguucaau agaccuaaca aguauacaaa cuuggguggu agacgaauua     1140

gcaaacguua uaacauuaaa cacggaaauc ccauccacau uagccacacu aaaaaucaaa     1200

auaccagaca uagaucaaca aauuuuaaau uuuguagaaa acaacuucua cgaacuaaag     1260

aagaaaacaa gcaaaaacuc auggcauucg uucucacuua aaacauuaaa uauacuaaua     1320

ccagaacucu uaaacacaag cgaagaacag aauacgauau uagaaagacu uaacuuaaaa     1380

uucgauuuga aaaauaaaua ugcagaaaag aaccaaauuc cuguuaaaga auuacuagaa     1440

caaauuuaua auccaaccgu uacaaaaucc guacaccaau cuauaaaacu auucaaugaa     1500

auaauagaaa aauacgguaa aaauaauauu ucauauguaa ccaucgaaau gccaagagac     1560

aaaaaugacc aagaucaaaa agauacaauu aaacgaauua acaaacucaa cucagaaaga     1620

aagaaugacu ccgaaaaaua uuucuuagaa agaucaggau gggacacaau aagauucgaa     1680

ucagcccuaa agaaaccuag auucgcugaa aaauuacucu ucuacuauga acaaaaagga     1740

aaaugugcau acaguggaca ggacauauua gcagaaucac uacuaaacga aucaugugaa     1800

gucgaccaca ucauaccacu aucaauaucg uuugacgacu cgauccacaa caaaguucuu     1860

guuacagcau caucaaauca agaaaaagga caaagaaccc cauacauggc cuuuauagau     1920

ggagcaaaac uaggucaaag cugggaugau uucguugccu ggguaaacuc uugcggauuu     1980

caucgaaaga aaguacaaaa cuuacucuac gaagaaaaua ucuuuaaccc agacguacaa     2040

agaggauucg uuuccagaaa cuuaaacgau acgagguaug caagcagaau aguacugaau     2100

gccauccaau cuuucuucua uaaacaaaaa gaucuaacaa aaguuaaagu aguaaacgga     2160

ucauucacac auacacuacg aaagaaaugg ggaucaucac uggauaaaaa uagagacacu     2220

uaccaccauc acgcaguuga cgcgacccug ugugccguaa cgcccuuuau uaacaucgua     2280

aaauacgagu ucuucgaaga agacgguaua aaauacaugg uagaccuaac aacaggggaa     2340

aaaguugcgu acucagauua caagaaaaug aaauuuuaug acaaaagagc auacguacca     2400

auaugggaug gauuuaucaa acaacucaca ccggcuaacc uauaccccaa aauaaaauuu     2460

ucacaccaag uugacucgaa auggaauaga aaaauaagug augccacgau auauaguacu     2520

cgcaaaauag uggaaacaaa aauaaacaag aaaggaguag caaaagaaac uguaaaagac     2580

auaguaaucg acaaaauaaa agacauauau acaccagaag gauggagaaa auucaaagaa     2640

aaacaagaua aacuauuaau gaaaaauuuc gacgagaaaa cauacaacaa auuaaucaaa     2700

auugccaaag aauaccccga aaagaaagaa guacaaacau ccaaugguaa agucagcuua     2760

guagaaguau caccauuuaa aauguauugc gaagaacaaa auguaccagc uauucgaaaa     2820

uacucaaaga aaggaaaugg cccauacaua cggucgcuca aauuuuauga uaaagaauug     2880

ggcaaacaua uaaacauaac caaagauagc uacggaaacg auauugaacg uacaauacgc     2940

aacaagaaaa ucauacucaa aaaccaaaau uccuggcgaa cagaugugua cuauaauuca     3000

guaaauaauu gcuaugaacu guuaggcaua aaauacuguc accucaaauu uuaucguggc     3060

aaauauggaa uacccaaaag cauuuacgac gaacuaaaga aagaagauga aauaucaagc     3120

gaaucaguuu uuaaauuuuc cuuauacaga cacgauagag uaagauucga aaaagacgga     3180

gaauauauag aggcuuuauu cggaagcaaa aacgaaucaa acaaaaacua ccuugaacuu     3240

ucaccuauag acaaaaacaa auggcuauca aaagaaaaug uaaaauucuu cggagaagua     3300

gcugccucug gccgcuuuau caaaggcuua aagcccggua uaaaaguaau aaagcaauca     3360

acagacuaca ugggacaugu auaugcgaua caccaagaaa aauugaaauu cguacuauca     3420

gacgcuuga                                                             3429


<210>  5170
<211>  3435
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5170
augacaucua aacaagaaaa acauggagca cuaacauuug gacuugauau agguauagcu       60

ucaguaggau gggcaguuuu aggggaaucu agaauaguag aucuuggagu aagguguuuu      120

gacaaagcug aaacugcaaa agaaggugaa ucguuaaacu ugacgcggag aauggcaaga      180

uuacucagaa ggagacuacg aagacgagcu uggagauuaa cgaaauuagc gagguuauua      240

aaacgagaag gucuaauugc ggaugcaaac uuauuuaaac acaaaauaga gaaaaacacc      300

cccgucccca aaucaucuug gcagcuacgu guagauggau uggauagaca acuaaacgcg      360

gaagaauggg caagaguuau cuaccacuua uguaaacaua gaggauuuca uuggauaucu      420

agagcagaag aaaaagcagc ugaaggugau ucuaagggug aaggaggaaa aguaaaacaa      480

ggauuagcag ggacaaagaa auuaaugcaa gaaaaaucau auagaacagc agcugaaaug      540

guuuuagcug aauuuccaga ugcacagcgc aauaaacaag gagauuacag uaaagcauua      600

ucgcgaauac uacucggcga cgaauuaaag gcuuuauucg aacaucaaag aaaacuagga      660

aauccaguag caaccgaaaa auuggaagca gcaauauuag guaacggaga uaaaaagugu      720

gggcuauuuu gggcucaaaa cccagcuuua gcggggaaua aauuauuaga aaugcuuggu      780

agguguacau uugaaaagac agggggcgau gauggcaaag gggaauaccg agcaccgaaa      840

gcaucuuuuu cggcagaaag acauguuugg cuaacacacu uaaacaauuu aagaaucgua      900

guagacggaa caaccagacc acuaaaugaa gccgaaagac guguggcuuu gccgaugccu      960

uaucaacaag caggcgaucu uucguacaaa caauugggag gcgcauuaau caaggaagga     1020

uucuugcaga aagauggcuu uaaauuugca ggucuaccau acccaacuaa gcaagcaagu     1080

gaagaaaaag caaaagaucc agaaacaagu guuuuaugua aacuacccgc uuggcauaca     1140

uugcgcaaaa cuuuagaaaa gcauggauua gaaucagaau ggcagaaauu aucgacagau     1200

gcgcuagaug gaaaaccuca auuacuagac aauauagcau ggguacuuuc uguuuacaaa     1260

gacgaugacg aaguaaaaac ugaauuggga aaacuaaauc uacccaauac agaaaagaug     1320

auugaugcuu uacuagauau aagguuugau aaauuuucaa auuuaagccu uaaagcucuu     1380

ucaaaaaucg uuccauuuau ggaacaggga caacgauaug augaggcagu agcaaauauc     1440

cccgaauacg ggcaucauuc acaacuacac aaaguaggug caggcgaaca uaaauauuug     1500

ccaccauuuu auaaagaaag agauaaaaca ggacgaaugg uauuuaacga ugacauggac     1560

auaccuagga auccaguagu acuacgugcc cuaaaucaag cacgcaaagu cguaaaugca     1620

uuaauaaaag aauauggcag cccacacgaa guccauauag aaauggcaag ggaucuuuca     1680

aggccauuag augaaaggag agauauagaa aaacuucaaa aagaauuugg ugagagaaac     1740

aagaaagaca aagaacuauu cgcacaagau uuuaauauag ucgguacagu aaaaggaaaa     1800

caauucgaaa aauaucaauu guauagagaa caacagggga aaugcauaua uccuucgaug     1860

gacgaaaaac aaggaauaug cuguuacuca cuaaaaccau uagaucuaca ucggguauug     1920

uacgaagaag gauacgcaga aaucgaucac gcauuaccau auucaagauc auuugacgau     1980

agcaaaaaua acaggguacu ugugcuggca aaagaaaaua gggauaaagg caauagaaca     2040

cccuuugaau aucuaggcga uaaagauaac ucagagccau ggagacaguu uguagcauuu     2100

gucgaaggua auaaagcgua uagacuugca aaacgaucaa gacuucuaag aaaagacuuu     2160

ggagacaaag aagcuaaaga guuuagagaa agaaaccuaa acgauacgag guauauuugu     2220

aaauucuuca aaaauuaugu agagcaauac uuacaauuac acgaagauuc ggaagcaaag     2280

agauguguug uauuaucagg acagaugacc ucauuccuga gagcgcguug gggccuagug     2340

aaaguaagag gagacagcga ccgacaccac gcguuagaug cagcuguagu cgccgcaugc     2400

agucacggaa ugguaaaacg ucugagugau uacagucgaa gaaaagagcu ggagcaagua     2460

agggaaggcu uuguagaugu agauacagga gaaauaguaa auccagcgau guuccaacaa     2520

guucaagaac auuucccaaa uccguggcca cauuucagag acgagguaaa acuaagauua     2580

agcauugacg acccaacucu auuaagguca gaaauggaaa aauuugggac auacacgcca     2640

gaaagccuag aggcuuuaag gcccuuauuu guuagucgag caccgcaacg aagaaacuca     2700

ggugcagccc acaaagauac cauauaugca caaccaaaaa ggcucguagc acaaggaggu     2760

guaacacaaa aaguggcacu aaguucccuu accuuaaaag accuggauaa auuaauugau     2820

ccccauagaa acgaaaggcu guaugcugcg auacgagcca gauuagaaca acacggugga     2880

aaaggugaca aagccuuccc ugccucaaau ccauuaagaa aaccagacuc ggaaggaaau     2940

ccaacagguc ccauaguucg aggcguuacg auggugauug auaaacuuag uggaauaccu     3000

aucagaggag gaauagcuaa aaacgauagc auggugcgag uagauguuuu cacaaaggcg     3060

aacaaauuuc acuuaguacc cguauacgua caucacucug uguugggacu accaaaccaa     3120

gcuauuguag ggggaaaaga agaaucagaa uggacgcaaa uagacgacag uuuugacugg     3180

cauuucagca ucuauccuaa cgauuuaaua cggguuaccu uaaagcagga aggaagguuc     3240

ggcuauuacg caggauguga cagaaguacu gcagcuauac aucugugggc acaugauaga     3300

aaccaacaag ucggcaaaga uggguuaaua agaaguauug gaguuaaaac ggcacucuca     3360

auagaaaaau uuaauguaga uguauuagga aaccgguacc cagcaccucc ggagaaaagg     3420

cgagauuuag cguga                                                      3435


<210>  5171
<211>  3435
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5171
augaccucca aacaagaaaa cuugggggcu cugacauucg gacuagacau agggauugcc       60

ucaguaggcu gggcgguccu gggcgaaaau cgaauuauag accuaggggu uagauguuuu      120

gauaaagccg aaacagcaaa agaaggugaa agucuaaacc ugacaagaag aauggcaaga      180

uuacugagac gaagauuaag aagaagggca uggagacuca caaaacuagc acgucuucua      240

aaaagagaag guuuaauagc ggacgcuaau cuauuuaaac acaaaauaga gaaaaauacg      300

cccguaccaa aaaguaguug gcaacuacga guagauggcu uagaucgaca acuuaacgca      360

gaggaauggg ccagaguaau auaucaucua uguaaacacc gaggauuuca uuggauaucu      420

cgagcugaag aaaaagccgc agaaggagau aguaagggag aaggaggaaa aguuaaacaa      480

ggauuagcug gcacaaagaa auuaaugcag gaaaaauccu auagaacagc ggcugaaaug      540

guucuagcag aguucccaga cgcacaaaga aauaaacaag gagauuacag caaagcauua      600

ucuagaauac ugcuagguga ugaacuuaaa gcucuuuuug aacaucaaag aaagcuagga      660

aacccaguag cuacugaaaa auuggaagcu gcaauacuag gaaauggcga uaagaaaugu      720

ggauuauuuu gggcgcaaaa uccggcacua gcaggaaaua aacuauuaga aauguugggu      780

cgaugcacgu uugagaaaac ugguggagau gaugguaaag gcgaauaucg agcuccaaaa      840

gcaucauuua gugccgaaag gcaugucugg cuaacacauu ugaauaacuu aagaauagua      900

gucgacggga cuacaagacc ucuaaacgaa gcagaaagaa ggguagcauu accaaugcca      960

uaucaacagg cuggcgaucu aucauauaaa caacuuggag gagcauugau aaaagaggga     1020

uuucuacaaa aagauggauu caaauucgca ggauuaccau auccaacuaa acaagccuca     1080

gaggaaaaag caaaggaucc agaaacaagu guucugugua aacuaccagc auggcauaca     1140

cuuagaaaaa cacuagagaa acacggucug gaaagcgaau ggcaaaaacu aucaacagau     1200

gcacuagaug guaagccaca acuauuagac aauauagcau ggguguugag ugucuacaaa     1260

gaugaugaug aaguaaagac agaacuugga aaauuaaauc uccccaauac cgagaaaaug     1320

auagacgcau uauuagauau ucgcuuugau aaauuuagua accuaaguuu aaaagcauua     1380

ucuaaaauag uuccauuuau ggaacaaggu caaagguaug acgaagcggu agcaaacauc     1440

ccagaauacg gucaccauag ucaacuacau aaaguuggag cgggagaaca caaauaccuc     1500

ccaccauuuu auaaagaacg ggacaaaaca gguagaaugg uauucaauga ugacauggac     1560

auuccacgga auccugucgu auuaagagca cuaaaucaag caagaaaagu aguaaaugca     1620

cuuauuaaag aguaugguag uccucaugaa guacacauag aaauggcacg cgacuuauca     1680

agaccccuag augaacgucg agauaucgaa aaacuucaaa aagaauuugg agaaaggaau     1740

aaaaaggaca aagaauuauu ugcgcaagac uuuaacauag uuggaacagu caaaggaaaa     1800

caauuugaaa aauaucagcu uuaucgcgaa caacaaggaa aguguauuua uccaaguaug     1860

gaugaaaaac agggaauaug uugcuacagc cugaaaccau uggaccuuca uagagucuua     1920

uaugaggagg gauacgccga gauagaucau gcguuacccu acaguagaag uuuugaugau     1980

uccaaaaaca acagaguauu ggucuuggca aaagaaaaca gagauaaagg gaaucgaaca     2040

cccuuugaau accuuggaga uaaggauaac ucagaaccau ggcggcaauu uguugcauuu     2100

guagaaggua acaaagccua ucgacuugcc aaaaggucca gauuacuacg aaaggacuuu     2160

ggagauaaag aagcaaaaga guucagagaa cgaaaccuaa augauacccg uuacauaugc     2220

aaauucuuua aaaacuaugu agaacaauau uuacaauuac augaagacag ugaagcgaaa     2280

agauguguug uucuaagugg acaaaugaca ucuuuucuua gagcucgaug gggccuagua     2340

aaaguacggg gagauaguga caggcaccac gcauuagacg cugcaguugu agcagcuugc     2400

ucacacggaa ugguaaaacg auugucagac uauucuagaa gaaaagaacu agaacaaguc     2460

agagaaggau uuguagaugu cgacaccgga gaaauaguca auccagcuau guuucaacaa     2520

gugcaagaac acuuuccaaa cccguggcca cacuuuaggg acgaaguaaa acuacggcua     2580

aguauagaug acccaacacu acuaagauca gaaauggaaa aauucggaac auacacaccg     2640

gaaaguuuag aagcccuaag accacuuuuu guaucucgag caccacaaag aagaaacuca     2700

ggcgcugccc acaaagacac uauuuaugcu caaccaaaaa gacuuguagc acaaggaggg     2760

guaacacaaa aaguggcuuu aucuuccuug acacuaaaag aucuugauaa auuaauagac     2820

ccacauagaa augaaagauu auacgccgca auuagagcac gacucgaaca acacggaggu     2880

aaaggagaua aagcauuccc agcgucgaau ccauuaagaa aaccagacag cgaaggaaau     2940

ccuaccggac ccauaguaag aggaguuaca auggugauag acaaauuaag uggaauaccg     3000

auuagaggag ggauugcuaa aaaugacagu augguaagag uagacguauu caccaaagcc     3060

aauaaauuuc accuuguacc cguguacgua caucauucag uauugggucu accuaaucaa     3120

gcuauaguag gaggaaaaga agaaucagaa uggacacaga ucgacgacag uuucgauugg     3180

cacuuuagua uauacccaaa cgauuuaauc cgaguaacac uuaaacaaga aggccguuuu     3240

ggguauuaug caggauguga uagaucaaca gccgcaaucc accuaugggc acaugauaga     3300

aaucaacaag uaggaaaaga uggucuaaua agaucaauag guguuaaaac cgcacugagc     3360

auagaaaaau ucaacguaga uguguuagga aauagauauc cagcgccacc cgagaaaaga     3420

agagacuuag ccuga                                                      3435


<210>  5172
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5172
augaaaacua uauugggucu cgaccuagga acaaauucaa uagguugggc auuagucaaa       60

gaaacagaaa acagcaauga aaaaucagaa aucauaaaac uugguguaag gguaaauccc      120

uuaacagucg acgagaaaac uaauuucgaa gcaggucgcc cguuaucuac aaaugcagac      180

agaacggcca aacgcucagc ucgacgcaac cuucaacgau auaaacucag acgcaaaaau      240

uuaauagauc uccuaauaaa acaucgauua auagauaaag auaccccacu uacagaaauu      300

ggcaaaaaca cgacacacca aacucuagaa uuacgugcga aagccgcccg agaacgaaua      360

gaauuggaag accuagccag gguuuuccua gcaauuaaca agaaaagggg auaccggagc      420

ucaagaaaag uaaacaacga agaagaagga caaguaguag acggaauggc cguagccaag      480

aaauuauacg acgaaaaucu aacuccaggc caauacgccu acgaauuacu uucaaaaggg      540

aagaaauacg uacccgauuu uuaccgaagu gaccuacaag cugaauuuga cucaauaugg      600

gaauaccaaa aacaauuuua ugcagacauu cuugacgaug aacuuuauaa ugcccuaaaa      660

gggcaaggac aacaaaauag ccguaaacgc uuccuagcaa uaaaaggugu auauacagcg      720

gaaaacaaag gaaaacgcga ugaagucaaa cuucaacacu acaaauggag aucagaagca      780

auaacacaaa aacuaagcau agaagaagua gcuuacgucu uaguagaaau aaacaacgac      840

cuaaacaaaa guagcgguua cuugggagca auuuccgaua gaucuaaaga auuguacuuu      900

aauaaagaaa caguuggaga aaaccuaugg aaacaaaucc agaaaaaccc acacacaagc      960

cuaaagaauc aaguauuuua caggcaagau uacuuagacg aauuugaaca aauaugggaa     1020

acacaagcac aauuucaucc ccaacuaacg uuagcucuaa aagaacaaau ccgagaugua     1080

guaauauucu accaacggaa auuaaaaagc caaaaaggac uguugagcuu uugccaauuc     1140

gaaagcuggg aaauugaaag aaaagaugaa aauggaaacg ucauacuaaa uaaaacaaca     1200

caauuaccaa aaagacaaac gguuggacga cgagucgcac ccaaaucauc uccucucuuu     1260

caagaauuua aaauauggca aaacauaaau aaccuggaga uagcuaaaau aagugacgga     1320

aacucuaaaa acaagaaaga aacagaagcc cuaucagaug acgaaagaaa acuacuauuc     1380

gaagaacuca acuuacgagg caaccuaagc caaaaagaag uccuucaaau auuaggucua     1440

aaagacaaag aauauaaaac aaacuucccc gaaggacucg aaggaaauag aacaaacgca     1500

gcauuguuca acaucuauca acaaauagca gaaaaugaag gauacggaga uuggacgaaa     1560

aagucagcuc aagagauuaa ggaagaacua aaggcuguau ucccucaaau aggaauacaa     1620

gccgacauac uagacuucaa ugcagaacug gacggaaaag aguucgagaa ccaagcguca     1680

uaucaacuau ggcaccucuu auacucagca gaagaggaug acaaaauaaa cgaagaagau     1740

cagauaauau acggcaacuc agccguauca cucaaaaaga aacuauguga aaaauucgga     1800

uuuacacccg aauacgcgaa auggaucgca aauguauccc uacaagacga uuacggaaac     1860

cuaucaacaa aagcaaugcg aaaaaucauc cccuacuuaa uagacggaaa cgauuacucu     1920

gaagccugug cacucgcagg cuauaaucac ucgaacucgc ucacuaaaga agaaaaugac     1980

aacagggaaa gacuaaauaa acuagaacua cuacccaaaa acagucuucg aaauccagua     2040

guagagaaaa uauuaaauca aaugguaaac gugguuaauc aagucaucga aaccuaugga     2100

aaaccagaug aaguuagaau agaauuagcu agagaauuaa agaaauccgc ugaagaaaga     2160

gccgaaauga cuaaagguau aaaugaagca acacgccgaa augaagacau aaagaaacuc     2220

aucaccaaaa acuucggcau cccaaacccc accaaaaacg acguuguaag auaucgacuu     2280

ugggaagagu uagcgccccu agccuauaaa gauguuuuua caggccguca aaucaagaaa     2340

gaggaccuuu ucucauccaa aauugacaua gaacauauaa uaccaaaagc acuacuauuc     2400

gaugacuccu uuucaaauaa aacauuagcc uucagagaaa ccaaccuaaa gaaagcagac     2460

cggacagccu acggauuuau agaaagcgau uauaacgcaa cauuagacga cuacauacaa     2520

agaguugaaa cauuauacaa uaaugcuaaa ggaacaauau caaaagggaa acgaaacaaa     2580

cuucuaaugg cgcagaaaaa ccucccagac ggauuuauag aaagagauuu aagaaauagu     2640

caauacaucg caaagaaagc aaaaucuaug auagaaaaag uauuuaauga cguaaacgua     2700

accaguggaa guauaacaga ucgguuaaga gaagacuggg accugauaaa ugucaugaaa     2760

gaacuuaacu ucccaaaaua caaagcauua ggucuaacgg aaauagagga acguuacgau     2820

auaggacaag agaaaaccaa gaaaguagaa gucauuacag acuggacaaa acgcaacgau     2880

cacaggcacc acgcuaugga ugcauuaaca gucgcuuuca caacaaaaag ccauguacaa     2940

uaucuaaaua acuuaaauac uauucauucu uuaaaaggcg augaaguaga uuuagaacuc     3000

ucaaaacuau acggccuuaa aaauacuauc accgaaguag uaaauaagaa acgaaaauuu     3060

auuccuccca ugcccaauuu uagagaagag gcgaagaaac acauugaaac cauauuagua     3120

uccaucaaaa auaaaaacaa aguauauacc aaaaacauaa auaaaacuaa gaaagaaagc     3180

ggauaucaca caaaagaaca auuaacaccc agaggacaau uacacaaaga aacaguuuac     3240

ggaaaaucca aaagaccaau gaacaaaccu acaaaaauaa acaaaaauuu cucucuagaa     3300

caagcccaac uuauaauaaa uguucaagaa aaagagcuag uucugcgaca cuuagcccaa     3360

uucgauaaua auccagcagu cgccuuugac accaaaacuc ugaagaaaug ucacuaccua     3420

aaaaugguaa aucuuuga                                                   3438


<210>  5173
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5173
augucaggaa auacacaugg uccucuuacg uuuggauucg auauugggau ugcaucagua       60

ggauggugcg uccuagggga aaaccggaua guaaauuugg gagucaggug uuucgauaaa      120

gcggaaaccg cgaaagaagg cgaaucucua aaucuugcga gacgaacagc aagauugaug      180

agaaggcgac uuagaaggag agcuuggaga uuaaccaaau uagcacgacu acugaaaagg      240

gaaggauuaa uagcagaagc aucaaggaua cacaucccau cgccuguuag cccauggaga      300

cuaaggguag aaggcauaga caggcgucua acugacgaug aaugggcacg uguaauuuac      360

cauuuaugua aacaucgagg uuuucauugg auaagucggg cggaggaaag aaaagcagaa      420

agcgaugcua aagaaggagg aaggguaaag aaaggauuau cagauacuca aagaagaaug      480

acagaaaaag guuauaggag ugcugcugaa augguacuug cggaauuucc ugaugcucaa      540

cgaaauaaac aaggagacua uggaaaagca uuaagucgaa uccuuuuauc agaugaauua      600

gcuuugcuuu uccgaaggca aagagaauua ggaaacccaa augcugguaa cgaacuugaa      660

gccaccauuu uaggaaaugg gaacaggaaa aguggauuau uuugggaaca aaaaccaccu      720

cuagcuggug cagaucuguu aaaaaugcua ggcagaugua cauucgaaaa gaaugaauac      780

cgagcaccaa aggcuucauu uacagcggaa cgacacguau ggcuuacgag auuaaacaac      840

uuaagaguug uuguggaugg aacaacuaga ucccucgacg augcagaaca uagauuaguu      900

cuaccauuac cuuacaaaca agccggcgau uuuacauaca aacaauuaag aacugcauua      960

acgaaagcug gauuauuacc cgaaggauuc aaauucgcag gauuaaguua cccaacagga     1020

cgucaauuag augauccgaa agccaaagac ccagaaagug aaaaauuagu caggcuacca     1080

gcauggcagg aacuuagaaa aacacucaaa gauaaaaauc uagaaacaga augggaaggg     1140

auggcgggug cagccacagg aggaaaccca gaacuucuug augaaaucgc acgaguucug     1200

aguguauuca aagauggaga ugagguaaca gcggaacucu ggaaauuacc acuaccagga     1260

ggaaaggcua ugauagaugc guuaucugaa auaaguuuug auaaauuuca ugcauuauca     1320

uuaaaagccc ucagaaaaau aguaccccau auggaaucgg gccuacgaua cgaugaggca     1380

ugugcagugg cagguuauca ucauagccag uuacacgcug ccggagcugg agaacacaaa     1440

uuucugccgc ccuucuauuc aagacgagau aaagauggac gaauggucuu uaaugaagaa     1500

uuagauguac cccgaaaucc uguuguucua cgagcguuaa accaagcgag gaaaguauug     1560

aacgcccuag uacggaccua uggcucaccu acagcuguac acauugaaau ggcaagagau     1620

cuaucuagac cccuggauga aagaaacaaa guaaagaaag cucaagauga auaccgcgaa     1680

cguaacgaaa aagauaaagc ugcauuugca gcugaauaug guauugcccc uaaaucucga     1740

gaauuugaaa aauuucaacu auacagagag caacaaggaa aaugugcaua uucuuuaaaa     1800

agccuagauc ugcauagagu ccugcacgac aucggauaug cagaaguaga ucacgcucua     1860

ccguauaguc gaucauacga cgauagcaaa aacaauaaag uacuagcguu aacggaagaa     1920

aauagaaaua aaggaaauag aacagcuuac gaauacuuaa ccucauuucc aggcggagaa     1980

aauggagaaa gauggagaaa uuuuaguguu gccguugaaa caaauaaagc auacagacua     2040

gcaaaaagga cacgauuauu aagaaaagau uucaguggca gagcagcaga agaauuuaga     2100

gaacgcaauc uuaaugacac aagauacauc ugccgauucu uuaaaaacac aguugaacgg     2160

uuucuaaaau uagaugcaaa aguaggagca ggagauacag cagaaauuag ugcaaaaagg     2220

ugcguaguac ugaguggaca aaccacagca uuuuuaagag cuagaugggg auuacuaaag     2280

guaagaggua auagugacag acaccaugca uuagaugcug cgguaguagc agcguguuca     2340

caugguaugg uaaagagauu aggugauuau aguaggacua aggaauuagc uaaaguccau     2400

gauggauuuc cagacccaga gacaggagaa auaauagauc ccgugauguu ucaacaacuu     2460

caugcgcauu ucccagaucc cuggccgcau uuucgggcgg aacuugaggc uagacuaaac     2520

gucgaugaua cagccgauuu aagagcacga auggaauuau uuggcacaua cgccccugaa     2580

gcucuugaaa ccuuacgacc uuuauuugua agcagagccc cacagaggcg aaauucaggu     2640

gcagcccaua aagacacgau uuaugcucaa cccgaaagau uaaaagaaca agggggugua     2700

acacaaaaag uuccacucac cucguuaaca uuaaaagauu uagauaaauu aauagacccu     2760

cacagaaacg aaaaacuuua ugccgcaauc agggccaggu uagaagcaca uggaggaaaa     2820

gcagauaaag cauuucccgc aggcaacccu uugcgaaaac cugaucgcga aggaaauccc     2880

acugguccag uaguacgaac uguaacuaug gucauagaua aacuauccgg caucccugua     2940

agagguggua uagcuaaaaa ugacacaaug uuaagaguug auaucuuuag acacaagaaa     3000

gauggcaaau uucauuuggu accaguauau gucuaucacg caguugcaaa agaauuucca     3060

aaucgggcca uuguacaagc uaaagaugaa gaugaaugga cauuaaucga ugacaaauuu     3120

gauuuuuguu ucucugcgca uccaaacgac cugcuaaaga uaacacagaa aucuggagaa     3180

acaauauucg gguauuaccg aggaugucac ucagggaccg cugcaauaaa uauagcacua     3240

caugauagau augcauaugc cgagaaaaca acaaguuugg cauucucgaa aaagaacccu     3300

ggaaaaccga uaccuaauga caaacuuggc uuaauagaag gcauuggugu aaaaauggcu     3360

gccaauaugg aaaaguuuca uguagauaca uuaggaaaua uauauccagc accaccagaa     3420

caaagacgug ggcuagcaug a                                               3441


<210>  5174
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5174
auggcguaca gacuuggucu cgauauugga aucacaagug uaggcugggc cgucgucgcu       60

uuagagaaag acgaaucagg acuuaaaccu guuagaauac aagaccuagg cgugagaauu      120

uucgauaaag cagaggacuc aaaaacagga gcaucacugg cacucccaag acgagaggca      180

agaagcgcaa gacgaaggac ucgcaggagg cgccaucgac uauggagagu gaaacguuua      240

cuagaacaac auggaauauu aagcauggaa caaauagaag cacuauaugc acaacguaca      300

ucaucaccag augucuacgc acuaagagua gcgggacugg accgauguuu aaucgcagaa      360

gaaauagcga gaguauuaau acacauugca cacagaagag gcuuucaaag uaaucgaaaa      420

agugaaauaa aagacagcga ugcaggaaaa cugcuuaagg cugugcaaga aaaugagaac      480

cuaaugcaaa guaaagguua ucgaacuguu gcagaaaugc uaguaucaga agcaacaaaa      540

accgaugcag aagggaaguu agugcaugga aagaaacaug guuauguauc aaauguacga      600

aauaaagcgg gugaguacag acacacagua aguaggcaag caauagugga cgaaguaaga      660

aaaauuuuug cagcacaacg agcuuuaggu aacgacguua ugucggaaga acuagaagau      720

agcuauuuaa aaauucugug cucucaaaga aauuucgaug acgguccagg aggggauagc      780

ccauauggac acggaucagu aucaccggac ggaguacgac aaucaauuua ugaacgaaug      840

guaggaucgu guacauucga aacuggcgag aaaagagcac cccguucaag uuauucguuu      900

gaaagauuuc aacuacuaac aaaaguggua aaccuacgaa ucuauagaca acaagaagau      960

ggcgggaggu acccaugcga auuaacacag acagaaagag caagagucau agauugcgcu     1020

uaugagcaaa cuaaaauaac cuaugggaaa uuaagaaaac uacuagauau gaaagacaca     1080

gaaaguuuug caggacugac uuauggacua aauaggucua gaaauaaaac agaagauacu     1140

guuuucguag aaaugaaauu uuaucacgaa guucgcaaag cguuacagag agcuggaguu     1200

uucauacaag accuaucuau cgaaacguua gaucaaauug gcuggauacu aucaguaugg     1260

aaaagcgacg auaacagaag aaagaaacuu aguacacuag gacuaucaga uaacguaauc     1320

gaagaacuac uccccuuaaa cggaagcaaa uuuggacauc uuucauuaaa agcaauaagg     1380

aaaauacuac ccuuucugga ggauggcuau ucuuaugacg uagcaugcga acuagcaggu     1440

uaccaauucc aagguaaaac agaguaugua aaacaacgac uuuugccacc ccucggagaa     1500

ggagaaguua caaacccagu aguaagacga gcucuaucac aagccaucaa agucgucaau     1560

gcuguaauua gaaaacacgg cucaccggag aguauacaca uugaacuggc acgugaauug     1620

aguaaaaauc uagaugaaag gagaaaaauu gaaaaagcgc aaaaagaaaa ccagaaaaau     1680

aaugaacaaa uuaaagacga aauuagagaa aucuugggau cagcacacgu aacuggaaga     1740

gauauaguua aauauaaauu auuuaaacaa caacaagaau uuugcaugua uucaggagaa     1800

aaacuagacg uuacucgacu guuugagccc ggauaugcgg aaguagauca caucauucca     1860

uaugggauaa guuuugauga cucuuaugau aauaaaguuc uaguaaaaac agaacaaaau     1920

aggcaaaaag gaaacagaac uccuuuagaa uaucuccgag auaaaccuga acaaaaagcg     1980

aaauuuauag cauuggucga gagcauaccc cuaucacaga aaaagaaaaa ucaucuauua     2040

auggauaaaa gagccauaga ucuagaacag gaaggauuca gagaaagaaa ccuaucagau     2100

acucgauaua uaacccgagc gcucaugaac cacauacaag ccuggcuacu auucgacgaa     2160

acugccucaa caagaagcaa aaggguaguu ugcguuaaug gugccguuac agcauauaug     2220

agagcaaggu ggggcuugac caaagaucgg gaugcuggag auaaacauca cgcugcggau     2280

gcagucgucg uagcauguau aggggauucg uuaauucaaa gagucacaaa auacgacaaa     2340

uucaaaagaa augccuuagc agauagaaau cgauacguac aacaaguaag uaaaucagaa     2400

ggcauuacac aauaugugga uaaagaaaca ggagaaguau uuacauggga auccuuugau     2460

gaaagaaaau uucucccaaa cgaaccuuua gaacccuggc cuuucuuucg agacgaauua     2520

uuagcaaggu uaagcgauga uccaucuaaa aacauuagag cuauaggacu guuaacuuau     2580

ucugagacag aacaaauaga uccuauauuu guuagcagaa ugccuacgag aaaaguaacu     2640

ggcgcagccc acaaagaaac aauaagaagu cccagaauag uaaaaguaga cgacaauaaa     2700

ggcaccgaaa uacaaguagu aguaucaaaa guugccuuaa cagaacuuaa acuuacaaaa     2760

gacggagaaa uuaaagauua uuuuagacca gaagaugauc caagacuuua uaauacccua     2820

cgagaaagau uaguucaauu uggaggagac gcaaaagcag cauuuaaaga accgguauau     2880

aaaauaagua aagaugguuc aguaagaaca ccuguacgga aaguaaaaau acaagaaaaa     2940

cuaacuuuag gcgucccagu acauggaggu cgugguauug cagaaaaugg uggaauggug     3000

agaauagacg uauuugcuaa aggcggcaaa uacuauuuug uaccaauuua uguagcggau     3060

guguuaaaac gugaauuacc uaaccgccuc gcgacagcac acaaaccaua uagcgaaugg     3120

agaguagucg acgacucuua ccaauuuaaa uucagucuau auccaaauga ugcagucaug     3180

auuaaaccaa guagagaagu agauauaaca uacaaggaca gaaaagaacc aguagguugc     3240

agaauaaugu auuucguauc agcaaauaua gcgucagcaa guauuagcuu aagaacccau     3300

gauaauucag gagaauuaga aggauuaggg auacaaggac uagagguauu cgaaaaauau     3360

guaguagggc ccuuaggcga cacucaccca guuuauaaag aaagaagaau gcccuuuagg     3420

guagaaagaa aaaugaacug a                                               3441


<210>  5175
<211>  3441
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5175
auggcauaca gguuaggucu agauauugga auuacaucag uaggaugggc ggugguagca       60

uuagaaaaag acgaaucggg acugaaaccc gugagaauac aagaucuggg aguacgaauu      120

uucgacaaag cugaggacag uaagaccgga gcgucgcuag cacuaccucg uagagaagcu      180

cguagcgcaa gaagacguac gcgaagaaga agacauagac uauggcgagu gaaaagguua      240

uuagaacagc acggaauauu aucaauggaa cagauugagg cucuauacgc acaacgaaca      300

agcucaccag augucuacgc auuaagagua gccggccuug acagauguuu aauagccgaa      360

gaaauugcac guguuuugau acacauagca caccgaagag gauuucaaag uaauagaaaa      420

ucagaaauua aagacagcga ugcagguaaa uuguuaaagg ccguacaaga aaacgagaau      480

cucaugcaau caaaaggaua cagaacagua gcugaaaugc uaguaucuga agcaacgaaa      540

acagacgcgg aagguaaauu gguucaugga aagaaacacg gauauguaag uaacgugcgg      600

aauaaagcug gagaauauag acauacagua ucaagacagg caaucguaga ugaaguacga      660

aaaaucuuug ccgcacaacg agcuuuaggu aaugacguaa uguccgagga acuagaagac      720

ucauaucuua aaaucuuaug cagucaaaga aauuucgaug auggaccagg aggagauuca      780

cccuauggcc auggcucugu aucaccggau ggaguacguc aaucgauuua cgaaagaaug      840

guaggaucau guacauucga aacaggagag aaaagagcac cacgaaguuc auacagcuuc      900

gaaagauucc aauuguuaac gaaaguaguu aauuuacgaa ucuacagaca acaagaggac      960

ggaggcagau auccaugcga acuuacccaa accgagcgag cacgaguaau cgauugugcu     1020

uacgaacaaa cuaaaauaac auacgguaaa cucagaaaac uacuagacau gaaggauaca     1080

gaaaguuuug caggucucac cuacggacua aauaggagca gaaacaagac ggaagacaca     1140

gucuuuguag aaaugaaauu uuaucacgaa guuagaaaag ccuugcaaag agcaggaguu     1200

uuuauacaag acuugagcau agaaacucuc gaucaaaucg gauggaucuu gaguguaugg     1260

aaaucagacg acaaccgaag gaagaaacua agcacauuag guuuauccga caacguaauc     1320

gaagaauuac uuccauuaaa cgguuccaaa uuuggacacc ucagccuuaa agccauaaga     1380

aaaauuuuac cauuuuuaga agacggauac ucauacgacg uagcuuguga acuugcagga     1440

uaccaauucc agggaaaaac agaauacguu aaacaaaggu ugcuaccucc ucuaggagaa     1500

ggcgaaguaa caaauccagu aguuagacga gcacuaagcc aagcgauaaa aguaguuaau     1560

gcaguaauac gaaaacaugg aaguccugag ucuauacaua uugaguuagc aagagaacua     1620

aguaaaaacu uggacgaacg ccgaaaaaua gaaaaagcuc aaaaagaaaa ucagaaaaac     1680

aaugaacaaa uaaaagauga aauaagagaa auucuuggau cggcucacgu aaccgggaga     1740

gauauuguaa aauauaaacu auucaaacaa caacaggaau uuuguaugua cucaggcgaa     1800

aaauuagacg ucacaagguu auuugaaccu ggcuacgcag aaguggacca uauaauaccu     1860

uauggcauaa guuuugacga uaguuacgau aauaaaguuc uaguaaaaac ugaacaaaau     1920

agacaaaaag guaacagaac accauuagaa uauuuacgag auaaaccuga gcaaaaagca     1980

aaguucauag cacuuguaga aucuauaccc uuaagucaaa agaagaaaaa ccaucuauua     2040

auggauaaac gagccauaga ucuagaacaa gaaggcuuuc gugagagaaa cuuaagcgau     2100

acuagauaca uuacacgagc auuaaugaau cauauucaag cauggcuuuu auuugaugaa     2160

acagccucga cacgaucaaa aagaguagua ugcguaaacg gugcgguuac agcauacaug     2220

agagcaagau ggggauuaac caaagacaga aacgcugggg auaaacacca ugcagcagac     2280

gccgucgugg uggcaugcau aggagauucc cuaauacaaa gaguaacuaa auaugacaaa     2340

uuuaaaagaa augcauuagc agauagaaac agauauguac aacaggucuc aaaaucugaa     2400

gguauaacgc aauauguuga uaaagaaacc ggagaaguau uuacauggga aucauucgau     2460

gaaagaaaau ucuuaccaaa ugaaccauua gaaccauggc cuuucuucag agacgaauua     2520

uuagcacguu uaucagauga uccuucgaaa aacauacgcg cuauaggguu auuaacguau     2580

ucagaaacag aacaaauaga uccuauauuc guaucucgaa ugccgacgcg gaagguaacc     2640

ggugcagcuc auaaagagac aauuagaagu ccucgaauag ucaaaguaga cgauaauaaa     2700

ggaacagaaa uucaaguagu uguaucuaag guagcauuaa ccgaacucaa acuaacgaaa     2760

gauggggaaa uuaaggauua cuuuagacca gaagaugauc cacgacucua uaauacgcuc     2820

agagaaaggc uuguucaauu cggaggagac gcaaaggcug cauuuaaaga accagucuau     2880

aaaauuucga aagauggaag ugucaggacg ccaguaagaa aaguuaaaau acaagaaaag     2940

uuaacauuag gaguaccugu ucacggagga agagguauag cagaaaaugg ggguaugguu     3000

cgcaucgaug uguuugcaaa agaugguaaa uauuauuuug ugccaauuua uguagcagau     3060

guacuaaaga gggaacuacc aaaucgcuua gcuacagcac auaaacccua cagcgaaugg     3120

agaguagucg acgauucaua ucaauuuaaa uuuucauuau auccgaauga cgcaguuaug     3180

aucaaacccu caagggaagu agauauaacg uauaaagaca ggaaagaacc uguagggcuu     3240

agauuaaugu auuuugcugg ugcugacaua ucaggagcaa cccugacgcu ccgaacucau     3300

gacaauucug gagaauuaaa aggcuuaggc auucaaggac ucgaaguauu cgaaaaguac     3360

guuguaggac cuuuagggga cacacaccca guuuauaaag agcgccgaau gccauucaga     3420

guagagagaa aaaugaacug a                                               3441


<210>  5176
<211>  3447
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5176
augacaaaau uaaaucaacc auauggaaua ggacuagaca uuggaucaaa cucuaucgga       60

uuugccgugg uagaugcaaa cucccaccua cuaagacuua aaggcgaaac cgcaauagga      120

gcuagacucu uucgcgaagg acaaucagca gcagaccgcc gcggcucgag aacaaccaga      180

cguagauuau cucgcacaag auggcgacua ucauucuuaa gagacuuuuu cgcaccacac      240

auuacaaaaa uagauccuga cuuuuucuua cgccaaaaau auagcgaaau uuccccuaaa      300

gauaaagaca gauucaaaua ugagaaaaga uuauucaacg auagaacaga ugcagaguuc      360

uacgaagcau acccauccau guaucaccuu cgacuacgac uaaugacuga cgccucacaa      420

gcagauccca gagaaaucuu ucuagcgauu caucacauac ucaaaucacg aggacacuuu      480

cuaacaccug gcgcugcuaa agacuuuaac acagacaaag ucgauuuaga agacauauuc      540

ccagcauuaa cagaagcaua cgcacaaguu uauccugacc uggaauugac auuugaucua      600

gccaaagcag acgacuuuaa ggcaaaacua cuagaugaac aagccacacc auccgacaca      660

caaaaggcuu uaguaaaucu acuccuaagc uccgauggag aaaaagaaau aguuaaaaag      720

agaaaacagg uacugacaga auuugcaaag gccaucacag gacuaaagac caaauucaac      780

uuggcacuag gaaccgaagu agaugaagcg gaugcaucaa acuggcaauu cucaauggga      840

caauuagaug auaagugguc aaacauagaa acaucuauga cagaccaagg cacagaaauc      900

uucgaacaaa uacaagaacu uuauagagcg agauuacuua acggaaucgu accugcaggc      960

augucacuuu cacaagcaaa aguagcagau uauggacaac acaacgaaga ucuagaacua     1020

uucaaaacgu accuaaagaa acuaaacgac caugaacucg cgaaaacaau aagagucaua     1080

uacgaccgau acauaaaugg agacgacgcu aaacccuuuu uacgagaaga cuuuguaaaa     1140

gcauugacca aagaaguaac agcucacccc aaugaagugu cagaacaauu auuaaacaga     1200

augggacaag caaauuucau gcuuaaacaa cguacaaaag ccaauggagc gauacccgua     1260

caacugcaac aacgagaauu agaucaaaua auagcaaauc aaaguaaaua uuaugacugg     1320

cuagcuaaac caaacccugu ugaaaaacau cgcgcaagua aaccauauca gcucgaugaa     1380

cuccuaacau uuaggauucc cuauuacgua ggaccuuuaa uaacaccuaa agaacaagcc     1440

aaauccggag aaaauguauu cgcguggaug guaagaaaag accccucagg gaacauaacu     1500

ccauauaauu ucgaugaaaa aguagauaga gaagcaucag cuaacguuuu uauccaaaga     1560

augaaaacca cagacacaua uuuaauagga gaagaugucc uuccaaaaca aucucuacug     1620

uaccagaaau augaggucuu aaaugaauug aacaacguac ggguaaacaa ugaagcauua     1680

gguacugacc agaaacaaag acucauuaga gaagucuucg aaagacacuc cucugucaca     1740

auaaaacaag uagcugauaa ucuaguugca cauggagauu uugcacaaag accagaaaua     1800

cgcggccuug cagacgagaa aagauucuua ucaucuuuaa guacauauca ucaauuaaag     1860

gaaauacuac auggcgcaau agaugauuca acaaaacuuc acgauauaga aaacauaaua     1920

acauggucaa ccguauucga agacgcagca auauuuaaaa ccaaauuagc ugaaauagac     1980

uggcuagacg ccgccacaau uaagaaacuu ucaaacauua gguacagggg auggggucag     2040

uucucacgaa aacucuuaga cggauuaaaa cuaggcaaug gacaaacagu aauacaagaa     2100

cucauguuau caaaucacaa ucuaaugcaa auacuaacug acgaaacauu gaaaacuaca     2160

auggcggaac ucaaccagga uaaauuaaaa gccgaugaua uagaagaugu aauuaacgau     2220

gcauacacau caccauccaa caagaaagca cuaagacaag uucuuagagu aguagaagac     2280

auaaaacacg ccgcuaaugg ccaagacccu ucauggcuau acauagaaac agcugacggc     2340

ccaggaacag caggaaaaag aacucaauca agacagaaac aaauucagac agucuaugca     2400

aaugcggcac aagaauuaau agauucagca guuagaggag aacuagagga caaaauagca     2460

gacaaagccu cauucacaga cagauuagua uuauacuuua ugcaaggagg aagagacauc     2520

uacacaggcg caccacuaaa cauagaucaa cucucacauu acgacauaga ucauauccuu     2580

ccucaaaguc uuauuaaaga cgauagucug gacaacagag uucucgucaa cgccacaaua     2640

aaucgagaga aaaacaacgu auucaccuca accuuauucg caagcaaaau ggcugcaacc     2700

uggcgaaagu ggcaugaagc aggacuuaua ucaggucgaa aauuacgaaa ccuaaugcua     2760

cgucccgacg aaauagacaa guuugcacac ggauuuguag cuagacaguu aguagaaacg     2820

agacaaauua uuaaacuaac agaacaaaua gcagcagccc aauacccuaa uacaaaaaua     2880

auagcaguca aagcuggucu aucacaccaa uuaagagaag aacuggacuu ucccaaaaac     2940

cgagauguua accauuacca ccaugcauuu gaugcauucc uagcugcccg aauagggaca     3000

uaccuccuca aacgauaugc aaaacuagcc ccauucuuca cuuacgguga guucgcaaag     3060

guagacguaa agaaauuccg agaauuuaau uuuauuggag cucuuaaaca cgcuaagaaa     3120

aauauagucg cgaaagacac uggugaaaua guaugggaua aagaacguga cauacaagaa     3180

cuagauagaa uauacaauuu caaaagaaug cuuauuaccc acgaagucua uuucgaaacg     3240

gcagaccucu ucaaacaaac uauauaugca gcaaaagauu ccaaagaaag agguggcuca     3300

aaacaacuaa ucccaaagaa acgagguuau ccaacacaag uauauggugg cauacacggu     3360

aaaauaagac uaacauggag auggcuaaaa cuccuaagac gaaagaaaau gucaauagaa     3420

uuaucugaau uacaacuacu uaaauga                                         3447


<210>  5177
<211>  3450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5177
auggaaagau accacauagg acucgacaua ggaacaucau caauaggcug ggcaguuaua       60

ggagacgacu ucaaaauaaa acguaagaaa gguaaaaauc uaauaggcgu acgacuauuu      120

aaagaaggag auaccgcggc agaaagaaga ggauuccgaa cucagaggag acgccuaaac      180

aggcgaaaau ggcgauugaa acuguuggaa gaaauuuucg acccuuauau ggcagaagua      240

gauaaauacu uuuucgcgag guuaaaagag ucaaaccucu cgccgaaaga uucaaauaag      300

aaauacuugg guagucuacu auuuccggac auaucagauu caaacuuuua cgauaaauac      360

ccaacgauau accaucugcg acgcgaucuc auggaaaaag acaagaaauu ugaccuacga      420

gaaaucuauu uagcaaucca ccauauagua aaauaccgcg gaaauuuucu cgaaaaagua      480

ccagccaaaa acuacaaaaa cuccggagca agcauaggau uccuacuaga agaaguaaac      540

gacuuguacg gaaauauaau ugguaaugaa gauguagcaa uacucgacaa ugacaaauuu      600

gaagacguag agaaaaucau acuaaaugac gaaauaagaa acauagauaa acagaaaaau      660

guaggaagau uacucgugaa agacaagaaa gagaaaaaca uaguuacagc auucuccaaa      720

gccauauuug gauacaaauu uaauuuagaa gacuuacuac uaaucgaauc agaugagaaa      780

aauaaauuaa cauucaacga ugaaaacaua gaugauaucu ucaacgaacu aucucauuca      840

uuaaaugaca accaaaugga ccuacuaacu aaaacaagag aaaucuauuu caaauuuaaa      900

uugaacauga uaguaccaac aggauacacc aucuccgaaa gcaugauuga aaaauacgaa      960

augcauaaag cacaccuuaa aauguauaaa gaauuuauaa acacauuaaa cgcaaaagau     1020

aggaaaauac uuaaaaaugc auauagcgac uacauaaaua augaaaaagc aaaagcagca     1080

aaugcucaag aaaacuucua caaaaccguc aagaaaacaa uaaaagaaaa ugauucagau     1140

acagccaaga aaauaaucgg cuuaauagac gaaggaaauu ucaugccgaa acaaagaaca     1200

ggggaaaacg gugugauacc acaccaauua caucaaauug aauuagaucg aauaauagaa     1260

aaucaagcca aauacuaccc uuggcuggca gaagaaaacc caguagagaa aaauaggaaa     1320

uucgcaaaau auaaauuaga ugaacucgug acauucagag uaccauacua uguaggaccc     1380

cucauagaca aaacugaauc caauaaaaac gaaaaagaaa caaaauucgc cuggauggug     1440

agaaaagcaa aaggaacaau caccccuugg aacuucgaaa aucuaguaga caggacagaa     1500

agcgcaaaca gauucauaaa aaggaugaca ucaaaagaca ccuauauaau aggcgaagau     1560

guacuacccg caagcaguuu acuauacgaa aaauauaaag uacucaacga auuaaacaac     1620

aucaaaguaa acaaaaagaa acuagacgua gaacaaaaac aacacgucua ccuagacuua     1680

uucacuaccc gcaaaaacgu cacgaaagac gaucuagcca caucauuaaa cugugaugua     1740

gaaucaauaa caggucuaac agauaauaag aaauucaacu cgaguuuaag uaguuauaua     1800

gaccucaaag caauacuagg aaacaucgug gaugacuaua gcaaaaauga agacuuagag     1860

aaaauaauag aauacuccac aauauucgaa gacggaaaca ucuauaaaga aaaacuaucc     1920

gaaaucucau gguuaacaga cgaacaaauc gaaaaacuau caaacauuca cuucaaagga     1980

uggggaagac uauccaagaa acuauuaaca caaauaacaa acgaaaacgg cgaaaggauu     2040

auagacacac ucuggaauac caguaacaac uucauacaag uaauaucuga cgaaagcauc     2100

caagcaaaac uagcagaaau aaacggggaa uacgcgaaca aauacaacuu ggaagauaua     2160

cucgaugaag ccuacacauc accucaaaac aagaaagcaa uaagacaagu caugaaagua     2220

guagaagaca uugaaaaagc aaugaaaugu gaaccgaccu caauagcaau agaguucacu     2280

agagagaaaa ggaaaucaaa auuaacaaau acacgauaca agaaaauauc agagaccuac     2340

gagaaaauca cugaugaauu aauaagcgaa uacgaacuug gaaaauugca aucagaacua     2400

gacucuaaag uuaauaauau gagagauaga uauuaucuau acuucaugca auuaggacga     2460

gauauguaua caggggagaa aaugaauaua gacgaauuac accaaaaaua ugauauugac     2520

cacauauuac cacaaucauu cauaaaagac gacagccuaa acaacagggu acuaacuuca     2580

aaaucaguaa acaucaaaga aaaauccgau aaaacagcgg cugaccuaua cgccgcaaaa     2640

augggagacu uuuggcggaa acuacggaaa caaggccuaa ugacugaaca aaaauacaaa     2700

aaccuccuaa cgagaacuga uucaauaaau aaauauacua aacaaucauu uauuaaaaga     2760

caacuuguag aaacaaguca aguagugaaa cuagcagcaa auauacucca agacaaauac     2820

agaaauacaa aaaucauaga aauacgagcc agacucaaua gcgaucuuag gaagaaauac     2880

gaacuuauca aaaacagaga agugaacgac uaucaucacg caauagacgg uuacuuaacc     2940

accuuuguag gacaauaucu auacaaagua uaccccaaau uacgaucaua uuucgucuau     3000

gaugacuuua agaaauuaga uucaaacuau cucaaacaca uggacaaauu caauuucauc     3060

uggaaacucg aagauaagaa agcagaagac guauacgaca aagucaacga cgaguucguc     3120

cucaauguac cagaaaugaa agaguacauc cgaaagauau auaauuauaa auacauguua     3180

guaucaaaag aaguuacaac caaaaaugga gcauucuaug accaaaccaa auauaacgcu     3240

aaaacaauaa auuuaauccc aaucaagaaa gacaaaccua caaacaucua cggaggauac     3300

aaagguaaag uuagcucgua caugauguua guuaagaucc agaagaagaa agaaguaaua     3360

uauaaauucg uaggcguacc ucgccuaugg acugaugaau uagacaggcu aauugacacc     3420

gacgaaaaga aagcacuuuu aaagaaauga                                      3450


<210>  5178
<211>  3459
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5178
augaacgcug aacauggaaa agaaggacua cuuaucaugg aagaaaacuu ucaauaucgg       60

aucggauuag auauaggaau cacaagcgua ggaugggcag uacuacaaaa uaacagccaa      120

gaugaaccag uacgaauaac agaccuaggc guuagaauau ucgauguagc agaaaauccu      180

aaaaauggag augccuuagc agcuccaagg agagaugcaa gaacaacacg aaggagacua      240

agaagaagaa gacauagacu agaaaggauu aaauucuuau uacaggagaa uggcuugauu      300

gaaauggacu cauucaugga gagauauuau aaagggaacc uaccagacgu cuaucaacua      360

agguacgaag gacucgauag aaaacuuaaa gaugaagagu uagcacaagu uuugauacau      420

auagcgaaac auagaggauu uaggucgaca agaaaagcag agaccaaaga gaaagaaggc      480

ggggccgugu uaaaagcaac aacagaaaau caaaagauaa ugcaagaaaa aggauaccga      540

acaguaggug aaaugcuaua uuuagacgaa gcauuucaua cagaaugccu guggaacgaa      600

aaaggauacg uacucacacc aagaaaucgg ccagaugauu auaaacauac aauacuacgc      660

aguauguuag uggaagaagu gcacgcgauu uucgcagcuc aaagagcaca cggaaaucaa      720

aaagcaacag aaggacuuga agaggcuuau guagaaauua ugaccagcca gagaucuuuc      780

gacauggggc caggacuaca gccagaugga aaaccuaguc cauaugcuau ggaaggauuu      840

ggagacaggg uuggaaaaug cacauucgaa aaagacgaau auagagcacc gaaagcuacc      900

uacacagcag aauuauuugu agcgcuacag aaaaucaacc acacaaagcu aauagacgag      960

uucggaacag gaagauucuu cucagaagaa gaaagaaaaa caaucauagg auuauuauug     1020

agcucuaaag aguuaaaaua ugggacaaua agaaagaaau uaaacauuga cccgagcuua     1080

aaauucaauu cauuaaacua uagcgcaaag aaagagggug aaacagaaga agaacgggua     1140

cuagacacag aaaaagcuaa auuugcauca auguuuugga cauacgaaua cucuaaaugu     1200

cuaaaagaca gaacagaaga aaugccugua ggugaaaaag cugaucuauu cgacagaaua     1260

ggcgaaauau uaacagcaua caaaaaugau gauucuagau caaguagacu aaaagaauug     1320

ggacuaucag gugaagaaau agacgguuua uuagauuuga guccagcaaa auaucaaaga     1380

gucucacuua aagccaugag gaaaaugcaa ccuuaucuag aagauggacu cauuuaugau     1440

aaagcaugug aagcagcagg auaugauuuu agggcucuaa augauggaaa caagaaacau     1500

cuauuaaaag gagaagaaau aaaugcaaua guaaacgaca uaacaaaccc agucguaaaa     1560

agaucaguau cacaaacaau uaaaguuaua aacgcaauua uacaaaaaua uggcagccca     1620

caagcaguaa acauagagcu ggcucgagaa augucuaaaa auuuucaaga uagaacaaac     1680

uuggaaaaag aaaugaagaa aagacaacaa gaaaaugaga gagcaaaaca acaaaucauu     1740

gaacuuggaa aacaaaaccc aacaggacaa gauauccuaa aguauagacu auggaacgau     1800

caaggaggcu acugccuuua caguggaaag aagauuccac uagaagaguu auuugacggg     1860

ggauaugaca uugaccauau cuugccguac aguaucacau uugacgacag uuauagaaau     1920

aaaguauuag uaacagcaca agagaaucga caaaagggga aucggacccc guacgaauau     1980

uucggcgcag acgagaaaag augggaagau uacgaagcga gugugaggcu auuaguacgg     2040

gacuacaaga aacaacagaa auuacuaaag aaaaacuuua cagaagaaga aaggaaagaa     2100

uuuaaagaaa gaaaccuaaa ugacacaaaa uauauaacua gaguuguaua caauaugauc     2160

agacaaaauc uagaacuaga accauucaac cauccugaaa agaagaaaca aguaugggca     2220

gucaauggag caguaacaag cuaucuaaga aaaagauggg gauuaaugca aaaagaucgc     2280

ucaacugaca gacaccaugc aauggaugca guaguuauag caugcuguac agacggaaug     2340

auucauaaaa uaucaagaua uaugcaagga agagaauuag cauacagucg caauuucaaa     2400

uuuccagaug aagaaacggg ugaaauacua aacagagaua auuuuacuag agaacaaugg     2460

gacgagaaau uuggaguaaa aguaccucuu ccauggaaca gcuuuagaga ugaauuggac     2520

auaagacugu uaaaugaaga ccccaaaaau uuccugcuca cacaugcuga cgugcaaaga     2580

gaauuagacu auccagggug gauguaugga gaagaagaau cgccuauaga agaaggaagg     2640

uauaucaauu auauuagacc auuauuuguc agcaggaugc cuaaucauaa aguaacugga     2700

ucagcacaug acgcaacaau acggucagca agagauuacg aaacccgagg aguagugaua     2760

acaaaaguuc cacuaacaga ucuaaaacuc aauaaggaua acgagaucga aggcuacuac     2820

gacaaagaca gcgauaggcu acuuuaucaa gcgcuaguca gacagcugcu acuacauggu     2880

aacgauggaa agaaagcauu cgcagaagau uuucacaaac cgaaagcaga cgggaccgaa     2940

gguccagugg uaagaaaagu aaaaauagaa aagaaacaaa caagcggcgu gaugguuaga     3000

ggaggcacug gcauagcagc aaauggcgaa augguaagaa uagauguauu cagagaaaac     3060

ggaaaauauu auuuuguccc uguauacaca gcagaugucg uuagaaaagu auuaccaaau     3120

cgagcagcaa cacauacuaa accauacuca gaauggagag ugauggauga cgccaacuuc     3180

gucuuuucac uauauucuag agaucuaaua cacguaaaaa gcaaaaagga cauuaaaacg     3240

aacuuaguaa auggaggucu acuauugcaa aaagaaauau ucgcauacua cacaggagcu     3300

gacauagcaa cagcuucuau agcaggguuu gcaaacgaua guaauuuuaa auucagaggu     3360

uuggggauac aaagucuaga aauauucgaa aagugucaag uugacauacu cggcaacauc     3420

agcguaguca gacaugaaaa uaggcaggaa uuucauuga                            3459


<210>  5179
<211>  3462
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5179
augaaaaaca uacuaggacu agaucuugga uuaucaucga uaggcugguc gguaaucaga       60

gaaaacagcg aagaacaaga auuaguagcg augggcagcc gcgucguauc auuaacagcg      120

gcagaacuaa gcucuuuuac ccaaggaaac ggaguaucga uaaauaguca aagaacacaa      180

aaacgaacac aaaggaaagg auacgauaga uaucaacuaa gaagaacauu acuucgaaau      240

aaauuagaca cucuagguau gcugccugau gauucauuau cauacuuacc aaaacuacaa      300

cuauggggac uuagagccaa agcaguuacu cagagaauug aguuaaauga auuaggcaga      360

gugcuccuuc aucugaacca aaaacgagga uauaaaagca ucaaaagcga uuucucaggc      420

gacaaaaaga uuacagacua cguaaaaaca guaaaaacaa gauacgauga auugaaagaa      480

augagauuaa caauuggcga acuguucuuu cgaaggcuaa cagaaaacgc auucuuuaga      540

uguaaagaac aaguauaccc acgacaagca uacguagaag aguuugauug uauaaugaac      600

ugucaaagaa aauucuaccc agauauacua accgacgaaa caauaaggug cauaagagac      660

gaaauuauau acuaucaaag accccucaaa aguuguaaau aucuaguaag ucgaugcgag      720

uucgaaaaga gguuuuauuu aaaugcagcg ggaaagaaaa cugaagcugg cccaaaaguc      780

ucacccagaa caagcccccu auuccagguc ugcagauugu gggaauccau aaacaauaua      840

guaguaaaag acagacgaaa cgaaauagug uuuauauccg cagaacagag agcugcacuc      900

uuugauuucc uaaacaccca ugaaaaacuc aaaggaucag auuuauuaaa auuacuggga      960

uuaagcaaaa ccuacggaua uagauuaggc gaacaauuua aaaccgggau ccaaggaaau     1020

aaaacccgag uagaaauaga aagagcucua ggcaauuauc cugacaagaa aagauuacua     1080

caauucaauc uucaagaaga aagcuccagc auggucaaca cagaaaccgg cgagauaaua     1140

ccaaugauau cuuuaucauu cgaacaggaa ccacuuuaua gguuguggca cguuuuguau     1200

aguaucgaug auagagaaca auuacaaucg guauuaagac agaaauucgg aauugacgau     1260

gacgaaguau uagaaagauu aagugcgauu gaccugguaa aagccggauu cggaaacaaa     1320

aguucaaaag cuauccgacg aauacuacca uuucuccaau uggguaugaa uuacgcugag     1380

gcaugugaag cagcagguua uaaucauuca aauaacuaca ccaaagcaga gaacgaagca     1440

agagcucugc ucgaucgacu gccagcuaua aagaaaaacg aauuaagaca acccgucgua     1500

gagaaaauac uaaaucaaau gguaaauguc guaaaugccc ucauggaaaa auacggacgc     1560

uuugaugaaa uaaggguaga auuagcaagg gaguuaaaac aaucaaaaga agaaagauca     1620

aauacauaua aaucaauuaa caaaaaccag agagaaaaug aacagaucgc aaaaaggaua     1680

guagaauacg gaguaccaac aagaucaaga auccaaaaau auaaaaugug ggaagaauca     1740

aaacauuguu guauauacug uggacagcca gucgacguag gagacuuuuu acgagguuuu     1800

gauguagaag uugaacacau cauacccaaa ucauuauacu ucgacgacuc auuugcgaau     1860

aaagucugcu cguguagauc augcaauaaa gaaaagaaua auagaacagc auaugauuau     1920

augaaaucca aaggagaaaa agcacuaagc gauuacguag aacgaguaaa cacgauguac     1980

acaaacaauc aaauaaguaa aacaaagugg caaaauuuac uaaccccagu agacaaaauu     2040

aguaucgacu uuaucgacag acaauuaaga gaaucacaau acauagcaag aaaagcuaaa     2100

gaaauacuaa caaguauaug cuauaaugua accgcuacaa guggaucagu gacuagcuuu     2160

uuacgacaug uauggggaug ggacacagua uuacaugauc uaaauuucga ucgcuauaag     2220

aaagugggac uuacagaagu uauagaagug aaucaccgag gaagcguaau acgcagagaa     2280

caaauaaaag acuggucuaa gagauuugac caccgacacc augccauaga ugcccuaaca     2340

aucgcaugua caaaacaagc cuauauacaa cgacucaaua aucuuagagc agaagaaggu     2400

ccugacuuca acaaaauguc ccucgaaaga uauauacaaa gucaaccaca cuuuaguguc     2460

gcacaaguga gagaagcggu agaucgcauc cuagugagcu uccgagcagg uaaaagggca     2520

guaacaccag gaaaaagaua cauaagaaag aauagaaaac gaauuucagu acaaucggua     2580

uuaauaccua gaggugcacu aucugaagaa ucaguuuaug gcgugauaca cgucugggaa     2640

aaagaugaac aaggacaugu aauacaaaaa caacgcgcag uaaugaaaua ccccauaaca     2700

ucuauaaauc gagaaauguu agauaaagaa aaaguugugg auaaaagaau acaccgaaua     2760

uuauccgggc gauuagcaca auacaacgau aacccaaaag aagcguuugc aaagccagua     2820

uauauugaua aagaaugucg gauaccuauu agaacuguac guugcuucgc aaaaccugcc     2880

auaaacacuc uagugccgcu aaagaaagau gauaaaggua acccgguagc auggguaaac     2940

ccaggaaaua accaucacgu agcuauauau cgcgacgaag acggaaaaua uaaggaaaga     3000

acuguaacuu ucugggaagc ugucgaucgg ugccggguug gaauaccagc cauaguuaca     3060

caaccugaua cuauuuggga uaauauacua caacggaaug acauaucaga gaauguacug     3120

gaaucauuac ccgacguaaa auggcaauuu guauuaaguu uacaacaaaa ugagauguuc     3180

auauuaggaa ugaaugaaga agauuauaga uaugcgaugg aucaacaaga uuaugcacua     3240

cuaaacaaau aucuauauag aguacaaaaa uuaucaaaau cagacuacag uuuucgcuau     3300

cauaccgaaa caagugucga agacaaauac gacggcaaac cuaauuuaaa acucagcaug     3360

caaaugggaa aacuaaaaag agucucaaua aagagcuuac uaggucuaaa cccacacaaa     3420

gugcacauua guguacuagg ggaaauaaaa gagauaucuu ga                        3462


<210>  5180
<211>  3474
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5180
augaauuacu auaacgguuu cauaauaaca gaaaacgcua ugacauccaa acaagaaaaa       60

cacggagcac uaacauucgg gcuugacaua gggauugcca gcgucggaug ggcaguauua      120

ggagaaucuc gaauuguaga uuuaggaguu cgauguuuug auaaagcuga aacagcaaaa      180

gaaggcgaau cacugaaucu gacaaggcgc auggccagac uucuaagaag gagauugagg      240

cgcagggcau ggagacuuac uaaauuagcu agacugcuua aacgagaagg guuaauagca      300

gacgcaaauc ucuuuaaaca caaaauagag aaaaauaccc cuguaccaaa aaguucaugg      360

caacucagag uugacggcuu agacagacaa uugaaugcug aagaaugggc uagaguaaua      420

uaccaucugu guaaacauag aggauuucau uggauaucca gagcagaaga aaaagcggcc      480

gaaggcgaua guaaagguga aggaggaaaa guaaaacaag gauuagcggg aacaaagaaa      540

cuaaugcaag aaaaaucaua ucgcacagcc gcagaaaugg ugcuugcuga auuuccagac      600

gcacaaagaa acaaacaagg cgauuauuca aaagcucuga gcagaauauu gcuaggggau      660

gaacuaaaag cccuauuuga acaccaaaga aaacuaggga auccaguggc caccgaaaaa      720

uuagaagcag caauuuuagg uaacggugau aagaaaugug gccuauuuug ggcacaaaau      780

ccagcccugg ccggaaauaa auuauuagaa auguuaggaa gauguacguu cgagaaaaca      840

ggaggagacg auggcaaagg agaauauaga gcaccaaagg cuucuuucag ugcggaaaga      900

cacguauggc uuacacaucu uaauaaucua cgaauaguag ucgauggcac aacccgacca      960

uugaacgaag ccgaaagacg ggucgcacua ccuaugccau accaacaagc aggcgauuua     1020

ucauauaaac agcuaggagg agcauuaauu aaagaagguu uucuccaaaa agauggauuc     1080

aaauucgcag gauuaccaua uccuacaaaa caagcaucag aagaaaaagc aaaagaucca     1140

gaaacaagug ucuuguguaa auuaccagcg uggcauacuu uaagaaaaac auuagaaaaa     1200

cacgguuuag aaagcgagug gcaaaaacua ucuacagaug cauuagacgg aaagccacaa     1260

cuacuagaca acauugcgug gguuuuaucu guauauaaag augaugauga aguaaaaaca     1320

gaacugggaa aguuaaaucu uccaaacaca gagaaaauga uagacgcauu auuagacaua     1380

agauuugaua aauuuucaaa ucuaagucua aaagcauuau cuaaaaucgu accauuuaug     1440

gaacaaggac aacgauauga ugaagcaguc gcaaauauac cagaauacgg acaccauagu     1500

caacuacaca aaguaggagc gggagaacau aaauaucucc caccguuuua caaggaaaga     1560

gauaagacag gaagaauggu guuuaaugac gauauggaua ucccgagaaa cccaguuguc     1620

uuaagggcau uaaaccaagc aaggaaagua guaaacgcuc uuauuaaaga auaugguagu     1680

ccgcaugaag uacauauaga aauggccaga gauuuaagua gaccauuaga ugaacgucga     1740

gauauagaga aauuacaaaa agaauuuggg gaaagaaaua agaaagacaa agaacuuuuu     1800

gcacaggauu uuaacauagu aggaacaguu aaaggaaaac aauuugaaaa guaccaacuc     1860

uaccgagaac aacaaggcaa augcauauau ccuaguaugg acgaaaaaca ggggaucugc     1920

uguuauucgc uaaaaccacu agacuuacau agagucuuau acgaagaagg uuacgcugaa     1980

auugaucaug cauugccgua cucccguucu uuugaugauu cuaaaaacaa caggguauua     2040

guccuagcaa aagaaaacag agauaaaggc aauagaaccc ccuuugaaua ccuaggagau     2100

aaagauaauu cagaaccaug gcgucaauuu guagccuucg uugagggaaa uaaagcauac     2160

cguuuagcaa aacgaagucg guuauuaagg aaagacuucg gagacaagga agccaaagaa     2220

uuuagagaac gaaaccuuaa ugauacucgu uauauaugca aauucuuuaa gaauuaugua     2280

gaacaauauu uacaacugca cgaagacucg gaagcuaaac gaugcgucgu acuaaguggc     2340

caaaugacau cuuuucuaag agcaagaugg ggacugguaa aaguaagagg cgacucagau     2400

agacaccaug cucuagaugc ugcaguaguu gcagcaugua gucacggcau ggucaaacga     2460

cuaucagacu acagcagaag aaaagaauug gagcaaguuc gagaaggauu ugucgacgua     2520

gauacaggag aaauaguuaa ccccgccaug uuucaacaag uucaagaaca cuuuccuaac     2580

ccguggccac acuuuagaga ugagguuaaa uugagauuaa gcauagauga uccaacucuc     2640

uugagaagug aaauggaaaa auuuggaaca uacacaccag aaucacuaga agcauuacgu     2700

ccuuuauuug uaaguagggc accucaaagg agaaauagcg gagcagcuca caaagauacu     2760

auauaugcgc aacccaaaag acucgucgca cagggcggug uuacacaaaa aguugcccug     2820

agcucauuaa cuuuaaaaga ccucgauaaa uuaauagauc cucaccgaaa ugaaagauua     2880

uacgccgcua ucagagcuag acuagagcaa cacggcggca agggagacaa agcauuucca     2940

gcaaguaauc ccuuaaggaa accugauagu gaaggaaauc caacgggccc caucgugcga     3000

gggguaacaa ugguaauuga caagcuaucg gguauaccua uuaggggagg aauagcuaaa     3060

aaugacucaa ugguacgcgu agacguuuuu acuaaagcaa auaaauuuca ucuaguaccc     3120

guauauguuc aucacuccgu guuaggacua ccaaaucaag caauagucgg ugguaaagaa     3180

gaaucagaau ggacucaaau agacgauagu uucgauuggc auuucucaau uuauccaaau     3240

gacuuaauua gaguuacuuu aaaacaagaa gguagauucg gauauuaugc aggcugugac     3300

cgaagcacgg cagcuauaca ccuuugggca caugauagaa aucaacaagu aggcaaagac     3360

ggguuaauua gaucaauagg agugaaaaca gcacuaucua uagaaaaauu uaacguagac     3420

guacuuggaa aucgauaccc agcaccucca gaaaagagaa gagacuuagc cuga           3474


<210>  5181
<211>  3486
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5181
augaagaaaa uauugggacu ggacuuagga agugcgucaa uagggugggc auuaguagaa       60

gaaacagaac aaaugcaaua uaauauaguc ucacuuggaa guaggauaau accauacaac      120

ggaacagaag gacaggaauu ugaaaaagga accggagaau caagaaauag uuuaagaacg      180

aaagcaagaa cagccagaaa aggauacgau agauaccaac ugagaaggaa aaacuuggag      240

aaaauauuac uucaaaaugg cauguaccca aaugaacaag uaagagcacu acaaaaacuu      300

gaacugugga aacuaagagc cgacgcaccg aacguacaaa uauccuuacc ggaacuagga      360

agauuacuuu ucugguugaa ucagaaaaga ggauacaaaa gcuccagauc ggacgcaaau      420

uuagacaaga aagauacaga auaugucgcc acaguaaaau cuagacacca gaaaauaaaa      480

gaugcuggac aaacugucgg ucaauauuuc uacgggcaaa uauuggaaaa cgaauaccac      540

agaauuaaag agcaaguauu uccuagagag gcauauauag aagaguucga cgcaauagug      600

gcgaggcaaa aagaauauua ccccaccauu cuuaaacaag aacuaauaga uaaaauaaga      660

aacgaaguaa ucuacuucca aagaggacuu aaaagucaaa aaggacucgu uucagucugc      720

gaauuugaag gaagagaguu caauggacac uucggcggcc cgaaaguagc gccaaaaucg      780

aguccaauau uccaacuagg aaagauaugg gaaaacauua acaacauaaa aauagcugac      840

aucgaacuaa cagcugaaca aaaauuagua uucuuuaauu aucuuaacug ccaugaaaga      900

auaacagaag caaaaauaag agaauuacua aaacuaagca aagacucaag guugaacgga      960

caauugaaaa acgguuuaac agguaauaaa acguacucuc aaauagcaag uuguuuugga     1020

aacaagaucg gagaguacga aagacuauuu aaauuugaau uagaaguaau ugagagcgag     1080

aaacuugaau acacauacca ucccaaaucu cacauacccg ugggagagaa aagaagaaag     1140

uacauaucug aaaauguuga aaaagaaccu uacuaucggu uauggcauac uaucuauuca     1200

auaaaagaca aagacgaaug cagagcagca uuaaucaaaa auuuuggcau agaagaagaa     1260

auagcggaaa aacuagcaag uuuggauuuc agugcgggag gguuuggaaa caaaugcgcc     1320

aagguuauaa ggaaaauacu accguaccua auggaagggg auaaauauaa uguugcaaug     1380

gacuaugcug guuacaauca cucauuuagu caaacuaaag augaaaaccu ccaacgcgua     1440

cuauuggaua gcauaaauca ccuacccaaa aacucauuaa gacaaccaau uguagagaaa     1500

auacuaaauc aaaugauaaa ccugguaaac gaaguaauaa aacaaugggg uagaccagac     1560

gagauucgcg uagaacucgc aagaaaauua aaacaaucga aaacagaacg aaaugaugau     1620

gacaauuuca ugagaaagag agaaagagaa aacgagauaa ucaaggcaga ccuagcaaaa     1680

uacggauuaa aagcgacaag aaauaacgua auaaagugga gacuauauca ugaaaucgau     1740

gacaaagaua agaaacuuaa ugcaaggugc auauauugcg gacaacccau aagccuuaca     1800

gaagccauuu uaggaaacaa cguggaugua gaacacauaa uaccuaaaga caaauuauuu     1860

gacgacagcc aaaguaacaa aacgcuugcg caccgaucau guaacucggg caaaggaaac     1920

aaaacagccu acgacuuuau gaaagacaaa ccacaauuug aggaauacgu ugcuagagua     1980

gaagaauuau acaagaaaca uuuaauuuca cuuacaaaga aacgcaaauu ucugaugagc     2040

gaagauaaaa ucccaucuga uuuuauugau agacaacuga gggaaucaca auauaucgca     2100

agaaaaucaa gagaaauacu acaaacaguu ugcagaaacg uauggucuac ugauggagga     2160

guaacugcac accucagaaa auuaugggga ugggacgaug uaacuaugaa ucuacaaaug     2220

ccgaaauaca aaggaauagg gaugacagaa auaguggaau gggaaucaaa ccaugggaaa     2280

aaucaacaua cacaggaagu aauaaaagac uggucaaaaa gagacgacca cagacaucau     2340

gcuauagaug cgcuuguagu agcuugcacg agacaaggau acauccaaag gauuaauacc     2400

cuaaacucca gugaaacuaa agaugaaaug caccguaaag uagcgggguc cuuagucgag     2460

uucaaucaaa aacauacauu auuaaauaaa uaccuuauaa cagaaaaacc auuuaccaca     2520

ucagaagucg aagacguagu aagcaaaaua uuaguaaguu ucaaagcagg aaagaaagcu     2580

gcaacacccg gcaaaagaaa agugggaaaa cgaggaaaua aacaaguagu acaagacggc     2640

aucauaauac cacguggagc uuugcaugcc gauuccguau cuggaaguau uaaacaauau     2700

gacccuacag gcaacuuuga aaaccauaac guaauaaaau auaaaguagg aguuggaaaa     2760

gguuauuuau uugacggaaa agaugacaaa aagaaaauug auaaaacuau agacugcauc     2820

guagacaaga aaauuagaga cuuaauaaaa caaagagcgg aagaaagcaa uuacagccug     2880

aagaaaaugu ucgaaacucc gauauuauuu aaucaaagag caguauucuc cgugagaugu     2940

aaaaccggaa acaaagcagu gguuccaauc agaaacggau acguaguacc ugacaauaau     3000

caccacacgg ccauauacaa auaugacggc ggcuuaaaag aacaaugcgu uucguuaaug     3060

caagcgauag aaagaaaaag auacaaccuu ccuauaauaa ucaaaaaucc aaaagaaaua     3120

uacgacaaaa uaaccgaaau agcuaacauc aaucaacugc uauucgacag ucuaccgaaa     3180

cuagacuggg aauuuguaac gacacuccag aaaaacgaua uguuuauccu cgggauggac     3240

uacagcgcca cagaagaagc auuggcugac ggcaauuaca gacuucuaag cgaauacuug     3300

uauaaaguac aaaacauaag ugaugguaua uauagacuau gucaccauac aauaaccaaa     3360

uucgauuuaa gcaaagcaaa caaaccagac aaaagauucu acaacauaca aucauuaaaa     3420

gcauggaaua cacucaaccc acaaaaagua aaaauaaaca uauuagggca aauagaacga     3480

caauga                                                                3486


<210>  5182
<211>  3501
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5182
augcauguug aaauagacuu cccccacuuc ucgcggggug auucacaccu cgcaaugaau       60

aaaaaugaga uauuacgagg cucauccgua cucuacaggc uaggacuaga ccuaggaucg      120

aauaguuuag gaugguuugu aacacauuug gagaaaagag gugacaggca cgaaccaguu      180

gcauuaggac caggcggugu aagaauauuc ccugauggac gagauccaca aagcggaacc      240

ucuaacgcgg uggauagaag aauggcccgu ggagcuagaa aaagaaggga ucgauuuguu      300

gaacgacgua aagaauuaau agcagccuua auaaaauaca aucuacuacc ggaugacgca      360

agagaacgua gagcauugga aguauuagac ccauacgccu uaagaaagac agcauuaaca      420

gacacacucc cagcacauca cguaggcagg gcucuuuuuc acuuaaauca aaggagagga      480

uuucaaucaa auagaaagac agacucaaaa caaagcgaag auggcgcaau aaaacaagca      540

gcaucaaggc ucgcaacgga caaagguaac gaaacuuuag gaguuuucuu ugcagauaug      600

cauuuacgca aaaguuauga agauagacaa acagcuauac gugcagagcu aguuagauug      660

gguaaagauc auuuaacagg aaacgcaaga aagaaaauau gggcaaaggu gcgaaaaaga      720

uuauuuggag augaaguacu cccgagagca gaugcaccac augguguuag agcuagagca      780

acaauuaccg gcacaaaagc guccuacgau uacuauccaa caagagauau gcucagggau      840

gaauuuaaug caauaugggc cggacaaucu gcacaccacg cuacuauaac ugaugaagca      900

agaacagaaa uagaacacau aauauuuuau caaagaccgc ugaaaccggc uauaguagga      960

aaauguacuu uagauccugc aacacggcca uucaaagaag accccgaggg auacagggca     1020

ccauggaguc aucccuuggc acaacgauuu cgcauacuca gcgaagcuag aaaccuagaa     1080

auacgagaua ccgguaaggg aucuagaaga uuaacuaaag aacaaucuga cuuaguaguu     1140

gccgcacuau uagcaaauag agaaguuaaa uuugauaaau uaagaacacu gcuaaaguua     1200

ccagcagagg caagauucaa ucucgaaucu gaccgucgag ccgcauuaga cggagaucag     1260

acugcagcaa gacuaaguga uaagaaaggu uuuaacaaag cauggagagg cuuuccccca     1320

gagagacaaa uagcaauugu agcaagauua gaagagacag aagacgaaaa ugaacuuauc     1380

gcaugguuag aaaaagagug ugcauuggac ggagcagcug ccgcaagagu ugcaaacaca     1440

accuuaccag acggucauug ucgguuagga cuacgggcua uaaagaaaau uguuccuaua     1500

augcaagacg gguuagauga agauggaguu gcuggagcag guuaucauau agcagcaaaa     1560

agagcgggau augaucaugc aaaauuacca acaggagaac aauuaggaag auuaccauac     1620

uacggacaau ggcuucaaga ugcaguagua gguaguggag acgcuagaga ucaaaaagaa     1680

aaacaauacg gacaauuccc aaauccaacu guacacauag gacuaggcca gcuuaggagg     1740

guaguaaacg accucauuga uaaauacggg ccuccaacag aaauaucuau cgaauuuaca     1800

agagcccuaa aauuaucgga acaacagaaa gcagaaagac aaagagaaca aagaagaaau     1860

caagacaaaa auaaagcccg cgcagaggaa cucgccaaau uuggcaggcc agcaaaucca     1920

agaaaucucu uaaaaaugag auuaugggaa gaacuggccc acgacccacu agauagaaaa     1980

ugugucuaua cuggcgaaca aauuucuaua gagcgacuau ugucagacga aguggauauc     2040

gaucacauau uaccaguugc aaugacacuc gacgauucgc cagcaaacaa aaucauaugc     2100

augagauacg caaaccgaca caaaaggaaa cagacgccau cugaggcuuu uggcaguucg     2160

ccaacccuac aaggucacag guauaauugg gacgacauag cagccagagc aacaggcuua     2220

ccuagaaaua aaagguggag auucgaugca aaugcaagag aagaauuuga uaaaagagga     2280

ggauucuuag cucgacaauu aaaugaaacg ggaugguuag caagacuggc gaaacaauac     2340

uuaggagcag uaacggaucc uaaccagauu uggguuguac ccggaagacu cacaucaaug     2400

cuacgcggua aauggggcuu aaauggauua cucccuagcg acaauuaugc aggaguacaa     2460

gauaaagcag aagaauuucu agcaaguacg gaugauaugg aauuuagcgg ggucaaaaau     2520

agagcagauc auagacauca cgccauagau ggacuuguaa cugcacuaac agacagaagu     2580

uugcugugga aaauggcaaa ugcguaugau gaagaacaug agaaauuugu gauagaacca     2640

ccauggccua cuaugcgaga ugacuuaaaa gcugcauuag agaaaauggu agucagccac     2700

aaaccugauc auggaaucga aggcaaacua cacgaagacu cugcauacgg auuuguuaaa     2760

ccauuagaug caaccggauu aaaagaagaa gaagcaggua aucuggugua uagaaaagca     2820

auagagaguc ucaacgaaaa ugaaguagau agaauccgcg acauucaauu gaggacuauu     2880

gucagagauc acguaaacgu agagaaaacu aagggagugg cacucgccga cgcauuaaga     2940

caacugcaag cuccuaguga cgauuacccc caauuuaaac augguuuaag acauguaaga     3000

auccucaaga aagaaaaagg ugauuacuug guuccaaucg cuaauagggc cucaggaguc     3060

gcauauaaag cauacagugc gggagaaaau uucuguguag aaguauuuga aaccgccggu     3120

gguaaauggg auggagaagc aguaagacgu uuugaugcaa acaagaaaaa ugcgggacca     3180

aaaauugcgc augccccuca auggagggac gcgaacgaag gugcaaaauu aguaaugagg     3240

auacauaagg gggacuuaau cagauuggau caugaaggaa gggcaagaau aaugguugua     3300

caucguuuag acgcugcggc aggcagauuu aaacuagcag aucacaauga aacaggaaau     3360

uuagauaaaa gacaugcgac caauaaugau auugauccau uuagaugguu aauggcaagu     3420

uauaacacuu uaaagaaacu ggcggcgguc ccaguacgug uagaugaacu agguagagua     3480

uggcgaguaa ugccaaauug a                                               3501


<210>  5183
<211>  3507
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5183
auggcaagac cagcauucag agcaccaaga agagagcaug uuaauggaug gacacccgau       60

ccacaccgua uaaguaaacc cuucuuuauu uugguauccu ggcaccuuuu auccagagua      120

guaauagauu cauccagugg gugcuuuccg ggaacaagua gggaucauac ugacaaauuu      180

gccgaguggg aaugugcggu gcaaccuuau cgauuaaguu uugacuuagg cacaaauuca      240

auaggcuggg gauuacuuaa uuuagacaga caaggcaaac cgagagaaau aagagcacua      300

gguucuagaa uauuuaguga ugguagggac ccccaagaua aagcaaguuu ggcuguggca      360

cgucgacuag cacgacaaau gagaagaaga agagacagau auuuaacgcg aagaacgcga      420

uuaaugggag cauuagugag auucggauug augcccgcug auccagcagc aagaaaaagg      480

cuagaaguag caguagaucc guauuuagcg agagagagag caaccaggga aagguuagaa      540

cccuuugaaa uaggcagagc acuuuuucau cuaaaucaaa gacgaggaua caagccagug      600

cgaacagcaa caaagccaga ugaagaagca ggcaaaguua aagaagccgu ggagcgauug      660

gaagcagcaa uagcagcggc aggagcgcca acacuaggag cgugguucgc guggcguaaa      720

acuagaggcg aaaccuuaag ggcgcgacuu gcaggaaaag gaaaagaggc agcuuaccca      780

uuuuauccug cucgucguau gcuggaagcc gaguuugaua cacuaugggc ugaacaagcu      840

agacaucauc cggaucuacu aacagcagaa gccagagaga uccuaagaca uagaaucuuc      900

caccaacgcc cacuuaaacc cccaccugug gguagaugua cuuuauaccc agaugauggc      960

agagcaccaa gggcacuucc uagcgcacaa agauuaaggc uuuuccagga acuagccagu     1020

cucagaguca uucauuuaga ucuuucugag agaccacuga caccagcgga gagagacagg     1080

auuguagcau uuguacaagg acggccuccu aaagcaggaa gaaaaccagg aaagguacaa     1140

aaauccgugc cauuugaaaa acuaagagga cucuuagaac ucccuccagg cacaggauuu     1200

aguuuggaau cagauaaaag accugaguua cugggagacg aaacgggugc uagaauagcg     1260

ccagcauuug guccuggcug gacugcgcug ccacuggagg aacaagaugc cuuaguagaa     1320

uuauuauuaa cagaagcuga accagaaaga gcgauugcug cucugacggc aaggugggcu     1380

uuagacgagg cuacagcggc aaaauuagcg ggugcaacgu ugccggauuu ccaugguaga     1440

uacggaagaa gggccguagc cgaauuauua ccaguauuag aacgcgaaac gagaggagau     1500

ccagauggac gaguuagacc uauaagauug gaugaagcag uaaaauugcu cagaggugga     1560

aaagaccauu cagauuucag cagagaggga gcauuauugg augcuuuacc uuauuaugga     1620

gccguuuuag aaagacaugu agcauuuggg acagggaauc cugcagaucc agaagagaaa     1680

cgaguaggua gagucgcgaa uccaacaguu cauauagcau uaaaccaauu gcgucauuug     1740

gugaacgcaa uauuagcucg ccacggacga ccagaagaaa uaguaauaga acuagcccga     1800

gauuugaaaa gaagugcuga agauaggcgu agagaagaca aacgacaagc agauaaccag     1860

aaaagaaaug aagaaagaaa acgacuuauu uuaaguuuag gagagagacc aacaccccgc     1920

aauuuacuga aauuaagauu augggaagaa caagggccag uagaaaaucg cagauguccg     1980

uauucaggcg aaacaaucuc gaugagaaug cuucugagug aacaagucga uauugaucau     2040

auuuuaccau uuaguguauc acuugacgau ucagcugcaa auaaaguagu augcuuaaga     2100

gaagcuaaua gaauaaagag aaauagaagu ccaugggaag cauucgggca ugacagugaa     2160

cgcugggcag gaauacuggc gagagcagaa gcauuaccaa agaauaagag auggcgauuu     2220

gccccggaug cacuagaaaa auuggaaggg gagggcggcc ucagagcaag acaucucaau     2280

gacaccagac auuugucaag auuagccguu gaauauuuac gauguguuug ucccaaagua     2340

agaguuaguc ccggaagauu aacagcucuc uugagaagac gauggggaau agaugcaaua     2400

uuagcagaag cugacgggcc cccuccugaa guuccggcag aaacacuaga cccgagcccu     2460

gcagaaaaga auagagcaga ucaccgacac caugcacuag augcagucgu cauuggaugu     2520

auagauagau caaugguuca aagaguacaa uuagcagcag cgagugcgga aagggaagcu     2580

gcugcacgug aagauaauau ucgacgugug cuggaaggcu uuaaagaaga accuugggac     2640

ggauuuagag cugaacuaga aagacgcgca agaacaauag ucguaaguca ucgaccugaa     2700

cacgggauag guggcgcauu acauaaggag acagcauaug ggccaguaga uccuccagaa     2760

gagggauuua auuuaguggu aaggaaaccu auugauggac ugaguaaaga ugaaauuaac     2820

agcgucaggg acccuagguu aagaagggca cuaauagauc gauuagccau aagacgacgg     2880

gacgcgaaug auccagcuac agcauuagca aaagcagcag aagaucucgc agcacaaccc     2940

gcaucgagag gaauaagaag agucagaguc cuaaagaaag aaucuaaccc aauuagagua     3000

gaacauggag guaacccauc agguccaagg ucaggcgggc cguuucauaa auuacuccug     3060

gccggugaag uacaucacgu agauguagca uuaagagcug acgguagaag auggguuggg     3120

cauuggguua cacuauuuga agcucauggg gguagaggag cagauggugc ugcagcacca     3180

ccaagauuag gggauggaga aagauuuuua augagacucc acaaaggaga uugccuaaaa     3240

cuugagcaua aaggaagagu gaggguaaug caagugguga aacuagaacc aaguucaaau     3300

ucuguaguag uggucgaacc ucaccagguc aaaacugaua ggucuaaaca uguaaaaaua     3360

aguugcgacc aacuuagggc aagaggcgca agaagaguga cgguagaccc acuaggccga     3420

guaagaguuc augcaccagg cgcuagagua ggcauaggug gagaugcagg aagaacggca     3480

auggaaccag cugaggauau aaguuga                                         3507


<210>  5184
<211>  3513
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5184
auguccaaau acaacguagg ccuagauaua ggaauagcau caguaggaug gucuauagug       60

gacgcagaua aaaagaaaau aauagaucua ggaagccgaa ucuuuucaag cggaaacgca      120

gccgcuaauc aagaaagaag gggauuccga ggaacaagac ggcuaauaag acgcaggaaa      180

aacagauuaa cugauguaau aaacuucuuc ggcgaaaaga aauucaucga cacuaaagua      240

guggauaaaa acggccaugu acauuauaaa uucuccguaa auaauacaga aucuccuuac      300

gaacuuagag uaaaaggacu gucugaaaua cuaacaaaac aagaauuagu uauagcauua      360

uaccauauag uuaaacauag aggcauuagu uacgaucuug gugacuuaga agacgacgga      420

acaucaggag uaacagacua uaaaacgagc auaaauauua auagacaauu auuaaaugaa      480

aagacaguag gucaaauaca acuagaaaga cuaaaugaau uuggaaaagu acguggucaa      540

guagaagaaa acgaaaacgu aacacuacuu aaugucuuuc caucagccgc auacgucaaa      600

gaagcaacac aaauauuaac aaaacaacgu gaauuuuacc ccgaaauaac agacgacuuc      660

aucgcuaaau ucaugcuuuu caucacucga aaaagagauu acuuuguagg accaggaaac      720

gagaaaagcc gcaccgacua uggaauauau aagaaagaug gaagaacauu agauaaucuu      780

uuugaugaac uaauugguau agacaaaaua aauggagaga aaagagccuc agcuucgucu      840

uuaacagcgc aaauuuacaa uaugcugaac gacuuaaaua accuaacugu cccaaauaca      900

gaagacggca gauuaacaau ccaaaccaag aaagaaaucc uagaagcagc aaaaacgacu      960

aauggacucu uuggaauagc gcaaauaugu aaaauuaucg gauguaagaa agaugauaua     1020

aaagguuuua ggauagacaa aagugaaaag aaaaucaugc acacucuauc gggcuauaga     1080

aaauuucgaa aagaacuugc agacguuaac aucgaagcga augaccugaa cauuaacuug     1140

gucaacaaaa uagccgauau acuaacccuc aacaccgaaa aagcggaaau acguaaacaa     1200

uuagacaaaa guaacaugga uauuucaaau gaaaugaaag aaauaguaau aaauaaaaac     1260

aaagaguucc ugguagacgg gcgagccacg uggcacucau ucagcuauaa aacccuaaau     1320

uuacuaauac cggaacuauu aaauacacca gaagaacaga ugacgauccu aacuagacua     1380

ggguuaguaa aacccaacaa uaagaaauac gaaggauuga aaaauauacc cgcgagaaga     1440

auuacggaag aaauauacaa cccagucguc gcuagagcaa cccgagaagc aguaaacgua     1500

uuuaacgcua uaacaaagaa aguagggaga gauaauauca aagacguaau aauagaacua     1560

ccacgcgaag auaacgaagu agacaucaaa aagggaauag uuaaaagaca aaaacaaaac     1620

gaaguggaaa aagaaacugc agaugcauua guaaaacaac auaugacaau auccgauuca     1680

gcacuaauag cacaauauag aaaaauacga gggcuaucuc aaaaaguaag guauugguac     1740

caacaagaua cuauaugucc auacugcgga aagaaaauag aagcaguaca gcuuauaaac     1800

aauaacgacu cguuugaagu agaccauaua guaccaauau cgguaucauu cgacgacagu     1860

cagaauaaca aaguguuagu acacucacaa ugcaaucaag cgaaagaaaa acaaacacca     1920

cuaggauggc uaaacaacgg cggcggcuuc ggacaaucaa aagcagauua uauagcgaga     1980

gucaaaagca acaaaaauua ugccaaaaac aaaaucgaua auuuauuaaa ugaaacagac     2040

cuaaaugaua uagcaacaca aagaggauuu auacaaagaa auuuaaauga cacacgauau     2100

gcaucaagaa ucgucuuaaa ugaguucuac ucauucuucc guucaaauaa ucuaagcacu     2160

aaaguaaagg uaguaagagg aaaauggacu ucacaaaugc ggaagaaaug gaauggcguc     2220

gguggucugu caaaaaccag agacacauau caccaccaug cuauagaugc uugcauaaua     2280

gcaugcuuuc cauuguugaa ggcuuucgac aaagcaauua aauugauaga cguagaugga     2340

gaaacaggag aaaucuuaca agacaagaaa gcaauuaaac ucgcacaaga agcagaaauc     2400

guagagaaaa uaagcgucau caaaaauaag aaauuugaaa auaugguaaa ugaaauauac     2460

aacuucccac uauucaaaca gguagaacua gcaaaugaua uaacaaaucu cgaaaacccg     2520

guaaaauuca gucaccacgu agauaagaaa gcaaaucgag caguugcuaa ccaaacaaua     2580

uacggcacac ggauaaaaga aaaagaagua acaaaacgug gaaagaaaga gaaaauucaa     2640

gaaagauaca cacucggcac aauuaaaaau auauaugaca uagauggcuu caugaacuuu     2700

aagaaacugu acgacaaaau uagcaaaaau gacgauguaa aauuucugau gcagaaaaau     2760

gacccuaaaa caugggaaaa acucauagac auauucaacu cauacccuga uagcgaagaa     2820

guaacucaag ucgacggaaa aguaaaacga guagcaguaa gcccauucaa acuauauuac     2880

agagaaaaug gauucauaac aaaauacucg aaacauaaug auggcccaaa aguagucuca     2940

auaaaauauu acgauucaaa aguaggaagc cauauagaca uuacccccaa agaugcaaga     3000

aacaaaguaa uacuacaaag uuuaaaaccg uggagaacug acguauauuu uaacaguaua     3060

acacaacaau augaacuucu uggucuuaaa uauagcgacu uaaaauuuac gaauggagua     3120

uaugguauaa cacgagaugc cuacgaaaaa cuaaaacacg gagucuccga agacggagua     3180

acugcaugga aaccuauauc gaaaaauagu gaauuuagau uuaguuuaua ccgcaaugau     3240

cgaguacgag uaauagauga aaaaggugac ucaaucgaac ucuuauuugc aucacgaacu     3300

acauccaaug aaggauaugu cgaauuaaaa ccaauauaca aagcgaaauu ugauucaaaa     3360

gaagaaguag gauuuuacgg auuaguaagc aauggaagau uaaucaagaa aaugucgaaa     3420

ggaggauaca aauuauacaa agugaauaca gauaucuuag gcaaaccaua uuacgucacc     3480

cgagaaagcg augaaccaaa acuaucacuc uga                                  3513


<210>  5185
<211>  3516
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5185
auggcaaagu acagcgucgg acuagauaua gguauaucaa gcguagguug ggcaaucaua       60

gauauagaaa caaaacaaau aguaaaccuu gggaguagaa uauucccuuc aggaaacgca      120

gcaggcaacc aagaaagaag aggauuccgc ggaaucagga gacuaauacg aaggagaaaa      180

aauagacuaa cagaucuaaa aaaacuccuu ggagaaacau ucuucucaga cuuaaaaaag      240

auagauagca augguuauga auacuacgac auagacuuuc aaauaaaugc aaacccauac      300

gaacuacgcg uaaaaggaau aacagaaaaa uuaucaaaaa aagaauuagc agucguacug      360

caucacauaa uaaaacacag agguauauca uacgacuuag gcgaucuaga agaugacggu      420

acaucaggug uuucagacua uaaaaacuca auuaacauaa aucgccaacu acuacgagaa      480

aaaaccguag gacagauaca acuagaaaga uuaaucgaau uuaaccaggu acauggucaa      540

gucaaaaaag cugaggacgu gucucuauug aacguauuuc cgaguucagc auauagagac      600

gaggcaaaca aaauuaugau aaaacaacaa gaauuuuauu ccgaaauaac caacucuuuu      660

auggaacaag uggaccaacu aguaucaaga aaaagagacu auuuuauagg accaggaaac      720

gaaaaaagca gaaccgacua uggaauauac aaaagcgacg gucgaacacu ugauaaucuc      780

uuugcagaau uaauaggaac agacaaaaua aacggagaaa aaagagcgag cgcaucuagu      840

cuaacagccc aaguauacaa cuuauuaaac gaccuuaaua accuacacgu uucaaacacu      900

gaagauggca aauuaacaac aagcaccaaa aaagaaauaa uaaaagcagc gaaaacaacu      960

gacggcgugu uuggaauaag ccaaauaugc aaaauaauag gaugcaaaaa agaagagauu     1020

accggguuca gacaagauaa aucggaaaaa aaaguaauac acacacuagc agguuacaga     1080

aaaauaagaa aagcacuagc aaccauaaau aucgaagcaa augaacugaa ucccaccgua     1140

uucgaccaaa uugcagacau uuuaacccua aauacggaga aagccgaaau uagaaaacaa     1200

cuagauaaag uccaacuuga cauaacagau gagaugaaag aacuagucau ugauaaaaac     1260

aaagacuuuc ucguggaugg aagagcaaca uggcauagcu uuagcuauca aacuuuaaac     1320

augcuaauac cagaguugcu aaacacauca gaagaacaaa ugaccauucu gacaagaaug     1380

gggcuaauga agagcgacaa ugaaaaauac cgugggcuaa aaaaaauacc agcggaaaaa     1440

auagccagcg aaauauucaa uccaguaguu gcaaaaucaa ccagagaagc aaucaaagua     1500

uucaaugcaa uaaguaaaga aauaggcaaa aauaacauag agcacguugu uauagaauua     1560

ccgagagacg auaaugaaga agaugaaaaa agaaaaauuc aaaaauuaca aaaagcaaau     1620

gagacagaaa aagaagccgc agacaaccuc guaaaacaua aguuaggaau aucagacucg     1680

gcucuaauag cucacuauag aaaauucaaa ggauuaucac aaaaaguacg auauugguac     1740

caacaagaaa cauauugccc auacugcggc aaaauaauag aagcaacaca acucauaaac     1800

aacaaugaua auuucgaagu agaucacauc auaccgauaa guguuagcua cgacgacucg     1860

caaaauaaca aagugcuagu acauagccaa ugcaaccaag ccaaagaaaa gcagacacca     1920

cuaggcugga ucaacaaugg aggagguuuc ggacaaagca aaaaugaaua cacaaauaga     1980

guaaaaaaua auaaaaaaua cucuaaaaac aaaguugaca auuuauugaa ugaaacagac     2040

uuaaccgaca uuaucacuca acgaucauuc auacaacgaa accucaacga cacaagauau     2100

gcagccagaa ccguucuaga cgaguucuac ucuuucuuua agucaaacga cuuaccaacu     2160

aaaguaaaag ucguccgugg aaaauggaca agccaaauga ggagaaaaug gaacaaugua     2220

ggcgguuuaa gaaaaacaag ggauacacac caccaccacg ccauagacgc aagcauaaua     2280

gcaucauucc cacuguuaag agcauuugac agagccauaa aacucaucga cguugacaaa     2340

gaaacuggag aaauagugca auauggcaaa gcaagcuuaa acggagaugu caucgaagaa     2400

aaaucaguga uccaagcacc aaucauuaaa aaugaaaaau uugaaaaacu gguagaugaa     2460

aucuauaacu ucccacuauu caaacaagua gaauuagcca auaacauaga aaacccagaa     2520

aaucccguca aauucucaca ccauguugau aaaaaagcua accgaacagu agcgaaccaa     2580

acaauauacu ccacaagaau aaaaaaaaca gaaaaaauag uaagaaagaa aguaacagca     2640

aaagaagaag aauacguuau cggaacaaua aaagacauuu augacgugac aaauuuugaa     2700

aaauucaaaa aacuuuauga ugaaucaucc aaaaaagaug acauaaaauu cuugaugcaa     2760

aaaaacgauc cgaaaacaug ggaaaaauua guugaaauaa ugaacacuua uccagauuac     2820

gaagaaauuu cccaacuaga cgguaaagug aagagaguca agguaagucc uuuuaaacua     2880

uauuuccggg aaaaugguuu cauaacaaaa uaugcaaaac auaacaacgg gccaaaggug     2940

guaaguauua aguacuauga cucaaagcua ggcaaccaca uagauauaac accugucaau     3000

gcaaacaaua aaguaauauu acaaucucua aaaccaugga gaacugacgu auacuauaac     3060

aacgaaacuc aacaauacga acuccuagga cuaaaauacu cggaccuaaa auuuacaaaa     3120

gguguuuacg gaauuacaag agaagcauau gaaaaauuaa aauacggcua cacagacggc     3180

guaccggcau ggaaaccuau aucuaaagag uccgaauuua aauuuucacu auaucgaaau     3240

gaucggguaa gaauaaucaa ugaagccgga gaaaaaaucg accuucuauu cggcucuaga     3300

aaugaaacga gcgaagguua cguugagaug aaaccaauau ucaaaacaaa uuuugacgau     3360

gcugaagaag caaguuuuua cgguaaaacu aguaacaaaa gacuaauaaa aaaaauggcg     3420

aggaaaggcu accaaaucua uaaaguaaau acagauauau uaggaaaucu guacuacaca     3480

gcaaaagaau caaacgaacc aaaauuagac uuauga                               3516


<210>  5186
<211>  3519
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5186
augagaaaac ccuacucaau aggcuuggac auuggaacaa auuccgucgg augggcagua       60

auaacagacg acuacaaagu ccccucaaag aaaaugagaa uacaaggaac aacagaucgc      120

acgagcauaa agaaaaauuu aauaggggca uuacuauucg auaacgggga aacagcagaa      180

gcuacacgau uaaaaagaac aacacgaaga cgauacacaa gaaggaaaua uagaauuaaa      240

gaauuacaga aaauauuuuc aucugaaaug aacgaacucg auaucgcauu cuuucccaga      300

cuaucagaau ccuuuuuagu auccgaugac aaagaguuug aaaaucaccc aauauucgga      360

aacuuaaaag augaaaucac auaucauaau gauuacccaa ccauauacca cuuacgacaa      420

acacuagcag acagcgacca aaaagcggau cuaagauuaa uuuaccuagc acuagcacac      480

auaauaaaau ucagagguca cuuuuuaauc gaaggcaauc uugacagcga aaacacagau      540

guacauguac uauucuuaaa cuuagucaac auauauaaca aucuauuuga agaagacaua      600

guagaaacgg caaguaucga cgcagagaaa auauuaaccu caaaaacuuc caaaucaaga      660

cggcuagaaa aucucauagc agaaauccca aaccagaaaa gaaacaugcu guucggaaac      720

cucgucucau uagcauuagg uuuaacacca aacuucaaaa ccaacuuuga acuccucgaa      780

gaugcaaaau uacaaauauc aaaagacagu uacgaagaag accuugauaa cuuacucgcg      840

caaauaggag aucaauacgc agaucuguuc auugcugcca agaaacucuc ggacgcaaua      900

cuacuuucag acauuauaac aguaaaaggc gcaucuacaa aagcaccacu aagugcauca      960

augguccaaa gauacgaaga acaccaacaa gaccuagcau uacugaaaaa cuuaguaaag     1020

aaacaaauac ccgaaaaaua caaagaaauc uuugauaaca aagagaaaaa uggcuacgcc     1080

ggguauaucg auggaaaaac aucacaagaa gaguucuaca aauauauaaa accaauuuua     1140

uugaaauuag acggaacaga aaaacucaua ucaaaauuag aaagggaaga uuuccucaga     1200

aaacaacgca ccuucgacaa uggaucuauc ccccaccaaa uacauuuaaa cgaacuaaaa     1260

gcaauaauaa gacgucaaga aaaauuuuac ccauuuuuaa aagaaaacca aaagaaaauc     1320

gaaaaacucu uuacauucaa aauuccuuac uacguaggac cacuagcgaa cggucagagc     1380

ucauucgcau ggcuaaaaag acaaucaaac gaaaguauua caccuuggaa cuucgaagaa     1440

guaguagacc aagaagcaag ugcgagggca uucauugaaa gaaugacuaa uuucgauaca     1500

uaccucccug aagaaaaagu acuaccuaaa cauagcccac uuuacgaaau guucauggua     1560

uacaacgaau uaacaaaagu aaaauaccaa acagaaggua ugaaaagacc gguauuccuc     1620

agcucugaag auaaagaaga aauaguaaau uuauuauuua agaaagaaag aaaaguuacu     1680

gucaaacaac uaaaagaaga auauuucagu aaaaugaaau gcuuucacac aguaaccauu     1740

uuaggaguag aagacagauu uaaugccagc uuagguacau aucacgaucu ccucaaaaua     1800

uucaaagaca aagcauuccu agacgacgag gcaaaccaag acauacuuga agaaauagug     1860

uggacacuua cuuuauuuga agaccaagca augaucgaac gaagauuagu aaaauaugca     1920

gaugucuucg aaaaaucagu ucuaaagaaa cuuaagaaaa gacacuacac aggaugggga     1980

cgacuaagcc aaaaacugau uaacgguaua aaagacaaac aaacaggaaa aacaauccua     2040

ggauucuuga aagaugaugg aguagcaaau aggaacuuua ugcagcuaau aaacgauagc     2100

ucguuagacu ucgcaaaaau uaucaaaaac gaacaagaga aaacaaucaa aaaugaaagc     2160

cuagaagaaa ccauagcaaa ccuagcaggu uccccagcca ucaagaaagg cauacuccaa     2220

agcaucaaaa ucguagacga aaucguaaaa aucauggggc aaaauccaga uaacauugua     2280

auagaaaugg caagagaaaa ucaaucaaca augcaaggga ucaaaaacuc uagacaaagg     2340

uuaagaaaac uagaagaagu acacaaaaau acuggaucaa aaauauuaaa agaauauaac     2400

guaucaaaua cacaacuaca aagcgacagg cuauaucuau aucuucucca agauggaaaa     2460

gauauguaua caggaaaaga acuugauuau gauaaccuau cacaauacga uauagaucau     2520

auaauaccac aaucauucau aaaagacaac uccaucgaua acacaguacu uaccacccaa     2580

gcgucaaacc gaggaaaaag cgacaaugug ccaaauauag aaacuguaaa caaaaugaaa     2640

ucauuuuggu auaaacaacu aaaauccggc gcaaucucac aaaggaaauu ugaccaucua     2700

acuaaagcug aaagaggagc acuaucugau uucgauaaag ccggauuuau uaaacgacag     2760

uuaguagaaa cuagacaaau cacaaaacac guagcacaaa uacuugacag cagauuuaau     2820

aguaauuuaa ccgaagauag caaaucaaac agaaacguaa aaauaauaac ucuaaaaagc     2880

aaaaugguua gcgacuucag aaaagacuuu gguuuuuaua aauuacguga agucaacgac     2940

uaccaucacg cacaagacgc auauuuaaau gcuguugucg gaacggccuu auuaaagaaa     3000

uacccuaaau uagaggccga guucguauau ggagacuaua aacauuauga uuuagcaaaa     3060

uuaaugauuc aacccgacuc uagccuaggc aaagccacca ccagaauguu cuuuuacagu     3120

aaccuaauga acuucuuuaa gaaagaaauu aaacuugcag augauacaau uuucacacgu     3180

ccacaaauag aaguaaauac agaaacagga gaaauaguau gggacaaagu aaaagauaug     3240

caaacaauuc guaaaguuau gaguuaccca cagguaaaua uagucaugaa aaccgaagua     3300

caaacaggag gguuuaguaa agaaucaaua uggccaaaag gcgacucaga uaaacuaauc     3360

gcaaggaaaa agagcuggga cccaaagaaa uauggugguu ucgauagucc aauuaucgcu     3420

uauuccguac ugguuguagc gaaaaacugc aaaaggaaaa auagcaaaac uaaaaauuau     3480

cagcgaacau guggaaacaa aaacaauggc acaagauga                            3519


<210>  5187
<211>  3522
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5187
augcgaccaa uagaaccaug gauucuuggc cuugacauag guaccgacuc cuuagggugg       60

gcaguauucu caugcgaaga aaaagggccu ccuacugcaa aagaacuuuu aggaggagga      120

guacgauugu ucgauagugg aagggacgca aaagaccaca caagccgaca agcugaaaga      180

ggggccuuuc ggagagcuag acgucaaaca cgaacuuggc cuuggagaag ggauagacua      240

auagcucugu uccaggcagc gggcuuaaca ccaccagcag cagaaacaag gcagauagcc      300

uuggcucuaa gaagagaagc aguaucgaga ccauuggcac cagacgcuuu augggccgcg      360

uugcuacauc uugcacacca cagaggauuc aggucaaaca ggauugacaa acgagaacga      420

gcagcugcua aagcacuagc aaaagcaaaa ccggcaaaag ccacagcgaa agcaacugca      480

ccggcaaaag aagcagacga cgaagcaggu uuuugggaag gggcggaagc ugcauuacgc      540

caaagaaugg cagcuucagg ugcaccuaca gugggagcac uauuagcuga ugaucuugau      600

agagggcagc caguucgaau gcguuauaau caauccgaua gagauggugu uguugcaccg      660

acuagggcuc uaaucgcaga agaacuugca gaaauuguag cuagacaauc aagugcauau      720

ccuggucucg auuggccugc ugucaccaga uuggugcucg accaaagacc auuaagaagu      780

aaaggagcag ggccaugugc auuuuuaccg ggagaggacc gcgcucuuag agccuugccg      840

acaguacaag auuuuauuau ucgucaaacu cuagcaaauu uaagauuacc uagcaccagc      900

gccgaugaac cuagaccacu uacagaugaa gaacaugcga aagcucuagc cuuauuaucg      960

acagcccguu uuguugaaug gccagcccua cgcagagcuu uaggguuaaa aagaggcgua     1020

aaauucacag cugaaacuga aagaaacggc gccaagcaag cggcuagagg gaccgccggc     1080

aaccuaacag aagcaauacu agcaccauua auuccaggau ggucaggaug ggacuuagac     1140

agaaaagaua gaguuuucuc ugauuuaugg gcagcaagac aagacagaag ugcacuacua     1200

gcauuaauug gugauccaag gggaccaacc agaguuacag aagacgaaac agcagaagca     1260

guggcagaug caauacagau agucuugccu acgggucgag caucgcuauc cgcgaaagcc     1320

gcgagagcaa uagcacaagc aauggcucca gguauaggau augaugaagc cguaacuuug     1380

gccuuaggac uacaucacuc ucauaggcca cgacaggaac gacuagcacg auugccauau     1440

uacgcugcag ccuuaccgga cguuggcuua gacggagauc caguuggacc cccaccagcu     1500

gaggacgaug gagcagcggc agaagcauac uaugguagaa uugguaacau aucaguucau     1560

aucgcucuaa acgaaacuag aaaaauagua aacgcguuac uacacagaca cggaccuaua     1620

cuaagauuag uaaugguaga aacaacaaga gaauuaaaag cuggugcgga ugaaagaaaa     1680

agaaugaucg cagaacaagc ggaaagagaa agagaaaacg cagaaauaga uguugaacua     1740

cguaaaucag aucguuggau ggcaaaugcc cgugaaagaa gacaaagggu uagauuagca     1800

agaagacaaa acaaucucug ucccuacaca uccacuccaa uaggacaugc ggaccuucuu     1860

ggagacgcau augacauaga ucauguuauu ccguuggcaa gaggaggacg agacagucua     1920

gauaauaugg uacuaugcca aucagacgcg aacaaaacua aaggugauaa aacuccaugg     1980

gaggcauucc acgauaaacc cgguuggaua gcacaacgag augacuuuuu agcuaggcua     2040

gauccucaaa cggcaaaagc acucgccugg agauucgcug augacgcagg cgaaagggua     2100

gcacgcaaau cagcugaaga cgaggaucaa ggauucuuac cuagacaauu aacugacaca     2160

ggguauaucg cgcgaguggc auuacgauac cucucguuag uuacuaacga acccaacgca     2220

guggucgcaa ccaacggccg ucuuacgggc uuauuaagac uggcauggga caucacaccg     2280

gguccagcuc caagagaucu ucuaccaacg ccucgcgacg ccuuaagaga cgauacugcc     2340

gcaaggagau uucuagacgg auuaacacca cccccauuag caaaagcagu agaaggcgca     2400

guacaagcuc gacuugcagc auuaggaaga agucgaguug cugacgcagg acuagcugau     2460

gcacuugguc uaacccuugc cucauuagga ggagguggua aaaaucgagc agaccaucga     2520

caccauuuua uugaugccgc aaugauagcu gugacaacua ggggauugau uaaucaaaua     2580

aaucaggcaa guggagcagg acgaauuuug gauuuaagaa aauggccaag aacuaacuuu     2640

gaaccacccu auccaacguu uagagcugaa gucaugaaac aaugggauca uauucaucca     2700

ucuauucgcc ccgcucacag ggaugguggg uccuuacaug cagcaacugu auuuggagug     2760

agaaauagac ccgacgcaag aguauuaguc caaaggaaac cagucgaaaa auuauuucuu     2820

gaugcuaaug caaaaccuuu accagcugau aaaauagcgg aaaucauuga uggauuugca     2880

ucaccaagaa uggcgaaaag auucaaagca cucuuagcuc gauaucaagc cgcgcauccu     2940

gaaguuccuc cagcccuggc agcauuggcu guagccagag acccugcauu ugggccgaga     3000

gguaugacag cgaacacugu aauagcggga agaucugacg gagacggaga agacgcagga     3060

uuaauaacac cauuuagagc gaauccaaaa gccgcaguga ggacaauggg aaacgccgua     3120

uaugaagucu gggaaauaca aguaaaagga cguccuagau ggacacacag aguuuuaacg     3180

agauuugaua ggacacaacc agcaccaccu ccuccuccgg aaaacgcuag auuaguuaug     3240

agauuaagaa gaggagauuu gguauauugg ccauuggaau caggagacag acuauucuua     3300

guaaagaaaa uggcaguaga ugggcgacua gcauuauggc ccgcaagauu agcaacagga     3360

aaagcuacug cacucuacgc acaacucuca uguccgaaua uaaaccucaa uggugaccaa     3420

gguuacugug uacaaagugc agaaggaauu agaaaagaga aaauuagaac uaccucuugc     3480

acugcauuag gaagauuaag acuaucuaag aaagcaacau ga                        3522


<210>  5188
<211>  3525
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5188
augggaaagu auucgauugg acuugacaua gguauaaguu cuguaggaug guccauaauu       60

gacaucgaaa aaaagaaaau aguagacuua ggaagcagaa uuuuucccuc gggaaaugcc      120

gcagccaauc aagaucgcag aggcuauaga ggcacaagac gacuaauuag acgucgcaaa      180

aaccgacucg cagacguaau aaacuuccuc ggcgaagaaa acuucauaga cacaaaaguu      240

guggaccaga augggaacau acauuauaaa cuacuaguaa acucuacaga aucaccauau      300

aaacuaagag uuaaaggccu aaguaaaaug cuaacaaaac aagaauuagu aauagcacuc      360

uaucacauag ucaaacacag agggauaagc uaugauuuag gagaccuaga agaugaugga      420

acuucaggag ucacagacua caaaacaagc auaaacauaa accgacaauu acuaauaaaa      480

aagacaguug gacaaauaca acuugagaga uuaaaggaau ucgguaaagu gcgaggccac      540

guaaaaaagg acgaaaacau cacccuacuc aauguauucc cuucaucagc uuauguuaaa      600

gaagccacac aaauacugac uaaacaaaga gaauucuacc ccgaaauaac cgacgauuuu      660

auaacgaaau uuauaccuuu cauaucaaga aaaagagauu auuucguggg uccaggcaac      720

gaaaaaagcc guacagauua ugggauauac aagaaagaug gcagaacacu agacaaccua      780

uucgaugaac ucauagguau agauaaaauc ucuggagaaa aaagagcuuc ugcaucuuca      840

gugacagccc aaauauauaa uauguuaaau gaccuaaaua accuaacagu gacaaacaca      900

gaagacggca aacuaacaac acaaacaaaa cgugaaauca uugaagcaga caaaacuacu      960

aacggacuuu uuggaauaac acagaucugc aaaauaaucg gauguaaaaa ggacgacaua     1020

aaaggauuca gaauagauaa aguagaaaag ccaauaaugc acacacuaag cggauauaga     1080

cgauucagaa aggaauuagc aaaaauaaac aucgaagcua acgacuuaga uaucaauuua     1140

guuaacaaga ucgcugacau acuaaccuua aacaccgaaa aagccgaaau acguaaacaa     1200

cuaaacaaaa gcaauaucga ugucuccaau gaaaucaaag aacuuauaau uaacaagaac     1260

aaggauuucc uagucgaugg acgagcuacu uggcauaguu ucagcuauaa aacacuuaac     1320

cuacuuauac cagaguugau gaacacaagu gaagaacaaa uaaccauccu aacaagacua     1380

ggauuaguua aaucuaacuc ugaaaaauac aaaggcuuaa aaaauauccc agccaggagg     1440

auaacugaag gcauuuauaa uccuguaguu gcaaaagcua cacgcgaagc uguaaaugua     1500

uucaaugcaa uagcaaaaaa aaucggacga gauaacaucu cagauguugu aaucgaguua     1560

ccacgagaag acaaugaagc agacauaaaa aaagguauag uaaaaagaca aaaacaaaac     1620

gaauuagaaa aagaagcagc ggacgcucua guuaaacaac agaugaccau aucagacagu     1680

gcgcuaguag cacacuauag aaaaguucgc gggcugucac aaaaaguaag auauugguac     1740

caacaaggua ccuguugccc auacugcggc aaaaauauag aagcggucca auuaauaaac     1800

aacaaugauu cauucgaagu agaccacauu auaccaauuu caguaaguuu cgacgacagu     1860

caaaacaaca aaguacuagu ucacucacag uguaaccaag ccaaagguaa gcagacacca     1920

cuaggauggc ucaauaaugg uggaggauuc ggacagaaua aaacagagua cgucgcaaaa     1980

guaaaaacaa acaaaaacua caguaaaaau aagauggaua acuuacucaa cgacauggau     2040

cuaucugaua uaguuacaca aagaggguuc auucaacgga aucuaaacga uaccagauac     2100

gcaagucgga uaguauuaga ugaauuuuac ucauucuuua aaucaaauaa ccuaucaaca     2160

aaagucaaaa uaguaagggg uaaauggacu ucacaaauga gaaaaaaaug gaacggagua     2220

ggcggacuaa aaaaaaccag agauacguac caucaccaug caauugacgc auccauaauc     2280

gcaaguuuuc cacuacuaaa ggucuuugau aaagcuauaa aauuaauaga uauagacaga     2340

gaaacaggag aaauacuaca agacaaagaa gcaauuaaaa ugucgcaaga agcgaaguua     2400

uuagaaaaaa uaccuguaau acaaaaugaa aaauuugaag aacuaauaaa cgaauuauac     2460

gacuuuccac uuuucaaaca aguagaauua gccaacgaua uaaccaaccc cgaaaacccc     2520

gugaaauuca gccaucacgu cgauaaaaaa gccaacagaa gcguagcgaa ccaaacaauc     2580

uauggcacga gaauaaaaga aaaagaaaua aauaaacgag gaaaaguaga gaaaguacag     2640

gaacguuaua uauuagguac aauaaaaaac auauaugaca uagacagcuu uaccucauuc     2700

aaaaaaauau acgacaagau gaguaaaacg ggagauguaa aauuucuaau gcaaaaaaaa     2760

gacccaaaaa ccuggggaaa acucguagaa auacuaaaua guuauccuga cagcgaagaa     2820

auaacacaag uagauggaaa aguaaagcgc guuaagguaa gccccuuuga acuuuaucgu     2880

cgagaaaaug acuuuaucac aaaauaugca aaacacaaug acggaccaaa aauaguauca     2940

auaaaauauu acgacagcaa aauaggaucg cacauugaua uaacacccau caaugcuaaa     3000

aauaaaguag uucugcaaag ccuaaagcca uggagaacag acguuuacuu caacucagaa     3060

accagacagu acgaacuauu aggguuaaaa uauuccgauu uaaaauuuac aaacgggguu     3120

uauggcauaa ccagcgaagc uuacgaaaaa uuaaaacaug gcauaaguga agacggaacc     3180

aaaacaugga aaccaauugg aaaaaauagu ggauucagau uuucucuaua caggaauaac     3240

agaguaaaga ucguagauga aaaaggagaa ucauuagagc ucuuguuugc aagucggacc     3300

accagcaacg aaggauaugu agaacuaaaa cccaucuaca aaucuaaauu ugacaguaaa     3360

gaagacgugg gauuuuacgg gcaaaugaca cccaguggac aauucgucaa aaaaauggcc     3420

agaguggggu auaaacuuua caaagucaac accgaugugu uagguaaccc auauuacaua     3480

acaaaagaau ccgaugaucc cgaacuaagu uuauacaaga aauga                     3525


<210>  5189
<211>  3528
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5189
auggaagaaa ugaaacaaac aggcaggaaa aaugaagccc ccaaauuaac agaucuaaau       60

uauagaauug ggcuagauau aggaauagca agcguuggau gggcaguucu agaaaacaau      120

agccaugacg aaccauucag aauaaucgau uuaggaguaa gaauuuuuga uacagcugaa      180

cagccaaaga caggugcaag ucuagcauua ccuagaagag aagcuagauc agcacgaagg      240

agaauacgaa gacggaggga uagaaucgaa cguauaaaga aacuauuuca agcagaagga      300

uugauuaaca uagacgaguu cgaagcuaga uacaaaauug cagguuuguc agacguguau      360

aaauuaagau augaagggcu agacagaaaa cucacagacg acgaauuagc acaaguuuua      420

uuacauauag caaaacauag agguuuuaaa ucaacaagua aagcagaaga agcggaaaac      480

gaaaaugacg cugcgaaaaa gaaagaagca ggagcuuuaa agaaagggau cuccaacaau      540

aagaaauuaa uggaagaaaa aggguacagg acugucggag aaaugaugua ccuggaugaa      600

aacuuucgaa cagacugucc auggaaugaa aauggauaua uucuuacacc ucguaacaaa      660

ucaggugauu acaaacauac cguacuaaga gauauguuag uggacgaagu aagagaaaua      720

uucaaaagcc aaagaucgcu agguaauaua caugccucag aagaacuaga aaaugccuau      780

cuugacauaa ugcuaucaca gagaaguuuu gacauggggc caggugcgcc aagcaaauau      840

gcaauggaag guuucgagga caggguuggg cuauguacau uugaagguaa aaauggugaa      900

aaacgcgcag cuaaagcagc auauacgagu gaauuauuug uagcucucga gaaaaucaau      960

cauacgaaaa ucgugaguga ugauggagca acgagguuuc uaacaacuua ugaaagaaaa     1020

ucaauaauag aauuaaugca caaacaacaa acagugaaau acgcagcggu aagaaaagca     1080

aucggauuag cagaagaaga caaauuuuau aacuuaaacu acuccccaaa agcaggaucc     1140

aagaaaucgc cggaagauac cgacuucgua aaauuggaaa acuaccauaa aauuagaaaa     1200

gcauuaggug aagacauagu aucugaacac uuaccagcug agaaaauaaa acuguaugau     1260

agcauagcga cuauauuaac auuauucaaa aacgacgacu caagaauaaa aagguuuaua     1320

gaauuaggca uaggcgagga auguuucgau ggacucuuaa guuugucccc uagcaaauuc     1380

cauaacuuau cauuaguggc caugggaaaa aucauaccau ucuuauuaga cggaaacacg     1440

uaugauaaag caugugaacu agcaggauau gacuuuaaag cggauaacaa aggcgaaaaa     1500

ucgguacuuc ugaaaggaaa gaacaucaca gacaugguaa augauauaac aaauccugua     1560

guacgaagau cugucagcca aacaauaaaa guaauaaaug cgauaauacu ggaauaugga     1620

agcccgcaag cagugaacau agaauuggca agagagauga guaaaaauuu ugacgaaaga     1680

agaaaguuaa aauccgauau ggagagcaga gagaaggaaa auaauaacgu aauaaaaaga     1740

gugaaagaau acaaacuaaa uccuaccggu caggauauug uaaaauucag gcuaugggaa     1800

gaacaagaua ggaggugccu auacucgggc gagaaaaucg auauuaauga cuuauuuucu     1860

agagauggug gauaugacgu agaucauauu cuaccauaua gcauaacguu cgacgacaca     1920

uacaggaaua aaguauuagu aaaagcuagu gaaaaucgac aaaaagguaa ccgaacacca     1980

uaugaauacu ucauggcuga aaaggaugac gacgagugga acguauacga gacucuagug     2040

agaaacacag uuaaggauua uaagaaaguc uuaaaacucc ucaagaaaca uauuacagaa     2100

gaugagaaau cagaauuucg agaacgaaac cuuacagaca caaaauacau cacacgaauc     2160

auuuacaaua ugauacgaca aaaucuaguu cucaaaccau aucuuaguuu agaaaaagaa     2220

agaaagaaac aagugauggc aguuaacgga aguauaacac auuaucuaag uaaacgaugg     2280

gguuuagaau caaaagauag aaggaccgau acacaucaug cacaagaugc uguaguaauc     2340

gccuguacua ccgacggaau gauuaacaaa aucagcagau auaguaaagg uagagaguua     2400

cuauauagua gaggauuuaa auuugucgau gaagaaacag gagaaauacu gucccgugac     2460

aauuuuacaa gagaggaaug ggaugagaaa uacggaguaa aaauuccaau acccugggaa     2520

cauuucaggg augaauugga uauacgaaug aguagauaca acgguacagg agacgauucu     2580

auagacaaaa caacaccaag agacuucauu uuaagccaca gagacgcaca uaaauggaua     2640

aauuauccag aauggauguu cguugaaggc agaccuggaa ggaggggagu uuuagaugga     2700

auauucguaa gucgcaugcc aaaacacaaa gucaguggac aagcccauga agaaacggua     2760

agaagcgcua aauauuauaa ugacggaggg ucaguauuug agaguggagg guauguagua     2820

acaaagaaaa gcauucaaga uauaaaacua aaagacggag aaauaaaaga guucuuuaau     2880

ccagauagug acagacuauu guacaaugcu uuaguagaca gacuaaauaa auuugaaaac     2940

gauccaaaga aagcauuugc cgaaccauuu uauaaaccua aagcagacgg cacacaagga     3000

ccaaucguaa agaaaauaaa aguauuugaa aaacaaagcu caggaguguu cguaagagac     3060

ggcaaaggaa ucgcugcgaa uggagcaaug guaagaaucg acguauucug cgaagaagau     3120

aaagguaaaa agaaauauua uuuuguucca auauauacgg cagacgucgu caaaaagaaa     3180

uuaccagaua gagcagcuac agcuaauaaa uuaauaagug aauggagagu aaugaaagaa     3240

gaaaacuuua uauuuucauu auauagccga gacuuaauau acauagaaag ugacaaagga     3300

guccaaguua aagacauaga uggaaaaauu agcaaacccg ugaaucuauu cgcauauuac     3360

cuaggggcag acacuucaac ggcgucaaua agcggaaaac uacaugacag cucggcgaac     3420

uucaggucaa uaggcauaca aggauuaagg cgauuugaua aaugucaagu agauguauua     3480

ggaaauauau cauuuguuaa uaaagagaaa agaaugccau ucaauuga                  3528


<210>  5190
<211>  3531
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5190
augaacaaag uucaggaaga cugcuauaaa aaaauaaaag aaaaacuagc ggauagagaa       60

uaccgcauag gacucgacuu aggaguaggc ucuauuggau augcaguagu aucacuuaaa      120

ggugacaugc cagaagacgu gauauuagca ggaaguagaa uuuucaaauc aucgauuggc      180

gcagcggaac gcagacacuu ucgagcacaa aggaauacuc aucgccacca uagagaaaga      240

augagauacu uauggaaacu acuugcggaa aaaaaccuug cccuacaacu accagaaaac      300

uuaaguaaaa aagagaauuu uacagauuuu gauaccucac gaaaacguuu ccccauagaa      360

guauuaagaa augauccuua cauauuaaga uauaaagcac uagacgagaa acucucacua      420

uuccaacuug gauacgcucu auaccauaua gcgaaccacc gcggaaccgc caguguaaga      480

aguuuccuag aagacgacga agcgaaaguu aaagaaaaca aggaaacagc aaaacuagca      540

aaaaacacaa aaaaaauaau guacgaaaaa aaguaucgga cauucggaga auaccuauac      600

agagaaagaa ucgaaaaauu ucgacagaau gaacguauga aggucacaaa caaaaaugaa      660

aacaaagaau uugcaguaac ccgugauauc auagaaaaag aaguagaaau gauauuagga      720

gcacaaaaac gcuacaacac agccguucua gcagacgauu auauugaacg aauaaaaaaa      780

gcaauauuuu acgaaacgga aaaauugaua ccagaacccg aauacugccc uuacuucaaa      840

gaugauaaaa gacuaccuag aucacacaaa cucaacgaau auagacgcau cuacgaagca      900

cuaaacaaua caagauacua uaucccggaa auagaagaaa aaacagggga aauacuaagc      960

agaaaagaaa aagaacaauu aagcaaggaa gaaaaagaaa aacuauuuaa cuuccuuuua     1020

aaaaacaaag agcuaaccga agccaaaguc aaaaaauuac ucaaccucuc agaaauggca     1080

aaaguucaac uucagggaag agacaaaaag aaccaaaaaa uaaaaggcua cgcauuaaua     1140

acacuagagu cauuaccauu cugguccaaa uuacccgaac aacagcaaga ugaguucuua     1200

uacgacugga acaaacuucc ugacgaaaaa cuuaaagauc gauuacgaaa cuauuacaaa     1260

uuaacugacg augaaauaga ugaaacauuu caaaacauag uauuaccauc aaauuacgcc     1320

ccacucggaa aaaaggcaau ggaaauucuu cuggaauaca uaaaaaacgg gcucaguuac     1380

auugaagcau uagacaaagc agcugacgaa aacaaaauaa agguugaaaa gcacgaaaua     1440

aaugaccugu ugccauacua uggaaaaaua cuccaagaau caacacagau gcccauagga     1500

aaagccuuuu ccaaacaauu cgcagccaaa aauuacaaaa aaccagauau aaauaaaaac     1560

gagcaagaau uuggaagaau agccaacccu gucguacacc aaacauuaaa ugaauugaga     1620

aaauuaauaa acgaaauaau aacaguauuc ggaaaaaaac cccuagaaau aucacuagaa     1680

acaagucgag aacugaaaaa aucucaaaag gaaagagagc uaauagcaaa acaacaagac     1740

auagacgaag aaaaaagaaa aaaaauaugg aagacauaua uagaaccaca uuuacaacaa     1800

auaaaaagua ggucagauaa acucaaaaaa uuguucguuu uugaacuuug ggaagaucaa     1860

aaacauaauu gcccauuuug ucuagaaaaa auaaauguug augauauaua cucacgagga     1920

guagacaaag aacaccuguu uccacuaaaa aaaagcgaag augacagcga gaauaaucua     1980

gugauagcac acaacaaaug caaugcgaga aaagcggaaa gagcgccauu cgacgcauuu     2040

ggacaugaaa guauuuggaa ugauauauuc agcaacgcag aaaaaaacuu accaagaaag     2100

cuauggagau ucagacaagg cuccuucgaa aaguuccuug aaaaaccccc uaugcucaaa     2160

agguucgaaa cugacaauag cuauaucagu aagauaucag cacgauaucu agcuugucuu     2220

uuugacaaac caagcaaaau auucugugug aagccauucc uaacagccca acuacgaaua     2280

gcauggcaac uagacgaaau aaugauuaga cuagcagaaa auaucauacu agacucagaa     2340

aaagacucau ucaauaaagg cggccuacaa aaaaaaaaaa ggauagacaa cagacaccau     2400

gcauuagacg cugugguuau agcuuaugcu agccgaggau aucauaacuu cuuaaaccga     2460

guacaugcga aagguuacaa aauuaauuau gaugaaaaaa cuuggcuauc ugaaacacug     2520

agaccaccgg gaaauaaaaa ccucgaagac uucaaaagcg gcauaaucaa cguuaugaua     2580

aacgcaaaca uaucaauaaa acaugaccau aauaauaaag gucagcuugu caaaagcaaa     2640

gcuuacaaac ucuacuauuc agagauaggg aaauauauaa ucacaacauu aaaaucagua     2700

ucugagauau uauucaaaca aaaagaaaaa ccccaagaua cuuugagaag agcauuaugu     2760

aaauuugaag aucguuugag ugauauuaaa gacaaaaaac uuaggaaaaa gcucgaagau     2820

aacauuacua uuuauaaauc aauagagaau gaaucagcac ucucgaaagc gaaacaagaa     2880

cuagaagaau ucgaccuaaa agcuaaaaaa gaagguaaaa aagguuauga aaaaauuaca     2940

gauauucuaa ucuauaaaaa agcaugcucc augcacggag gcaaauacau acacgugaaa     3000

aaccgaauag agaacaaauu cuuugcucuu aaagagccua cugaaauuga gacaggauuu     3060

ggauacgaca ccgccgacaa ccuaagccua gacuuauacc augacaacaa cggaaaacua     3120

uguggugaag uaauucgaaa aauagacuuc aauagaaacc gcguacccaa uuauaagaaa     3180

gaaggauaua uauuacuuga aagaauauau caaggcgaua cucuagaagu agacaucucg     3240

gacgacaaaa uuucacuuaa aaauaaagua ggcucagcgc ccgacaaaag aaccuucgua     3300

aaaguacuaa cauucaccga aucagauaga uaccuaaaaa acaagagaga ccaaauacaa     3360

auauucuucu gcaaccuauu aaaaucaacc uuaaaacaug acgauucuag caaacacgac     3420

gauaguuuuu acgucucaag uaugcaaaaa uacaacguua gaaaagucau acuaacaagu     3480

cuaggaauaa ucaaauauag aucaaaaauu cuaaaaaaca aagaaauaug a              3531


<210>  5191
<211>  3531
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5191
auguuauucc caaaaaaauu aagcaaagaa gaaguagccu auguuuuagu agaaauuaac       60

agcaauuuaa auaauaguuc caacuaucuc ggaaacauau ccgaucgauc aaaagaauua      120

uacuucaaua aacaaacuau aggccaauuc uuauacacga aauuaaaaga agacagacac      180

uauaguuuaa aaaauaaagu auucuacagg caagacuacu uagaugaauu cgaacgaaua      240

ugggaaacac aagcaaucuu ucauaaagaa cucacagaag accuaaaaaa acaaauacga      300

gacacaauua uauuuuacca acgaagauug aaaucccaaa aaggccuaau aucacuaugu      360

gaauucgaaa auaaagaaau aacaauugaa aaagaaggaa aaaccguaac caagcaaaua      420

ggcguucgag ucauacccaa aucuucucca cuauuccaag aauuuaaaau cuggcaaauu      480

uuaaacaaug uuauauguaa aaaaaaagga accagaaaaa aaaaagcagu aaccccagca      540

ccccuacuau ucgaacucuu uggaacuccc ucggaaacuu ucuuuucuuu ggauauggaa      600

ugcaaacaac uuuuauuuga agaauuaaac cuaaaaggaa acuugaaagc agauaaagua      660

auccgacuau uaggauauau accacaggaa ugggaaauaa acuacaauca auuagaagga      720

aaccgaaccc aaaaaucccu auaugaggca uacuuaaaaa uaauagagau cgaaggauau      780

gauauaaaaa cauuuuuacg uaucaaaucc gacaaagaag acugggaccu agaagaaucg      840

ccuuuaagcg caaaagaaau ucgcgaaaac auacaacaca uauuugaaac acuaggaaua      900

ucaaccgcaa uauuauacuu cgaugcaaca uuaccaauaa aagacuuuga gaaacaagcc      960

uccuaucaac ucuggcaccu auuguacuca uaugaaucag acaacucccc guccgguaau     1020

gaaacacuau auaaacucuu gcaagauaaa uucggauuua agcgagaaca ugccguauuu     1080

cuauccaaua ucucauucca agaugauuac gguagcuuaa gcgcaaaagc aauaagaaaa     1140

auauacccau acauuaaaga aaaucaauau ucgacugcau gugaauuagc uggcuacaga     1200

cacucaaaau uaucucucac aaaggaagaa uuaaacaacc gaccauuaaa agauuuaaua     1260

ucacccuuaa aaaagaauuc auuacgaaac ccagugguag aaaagggacu aaaucaaaug     1320

auaaacguag ucaaugcccu aaucgaaaaa aacucagaaa aagacgcaga aggaaacaua     1380

aucaaauacu uccacuucga ugaaauuaga auagaacuag caagagaacu caagaaaaau     1440

gcacaagaaa gagccgaaau gacccuuaac aucaaaaacg caacuauaga acaagaaaaa     1500

aucaaaucca uccuaauaag cgacuucggc aucaaaaacc cgacgaaaaa cgauauaaca     1560

agauacagau uauaccaaga acucgcacac aaugauuaua aggaucucua caccaacacc     1620

gacauuccaa aagaacucuu auuuagcaaa aauauagaca uagagcauau aguuccaaaa     1680

gcaagagucu ucgaugacuc cuucucaaac aaaacccucg cguaccgaga cuucaauuua     1740

aagaaaggag agaacacagc auuugacuuc aucgauucua gucucggaga agaagaacug     1800

aaucaauaua uagcaagagu agaaaacgcc uuuaaaacua aaaacauaag ugcuacaaaa     1860

uacagaaauc ucuuaaaaaa agcaacggaa auuaacgacg guuucauuaa uagagaucua     1920

agagaaacac aauacauugc aaaaaaagca aaagaucuac uauuucaaau cacacgaagu     1980

guagugucaa ccuccggcuc aauaacagac cgacuaagag aagacugggg acuuauugau     2040

auuaugaaag aguuaaacau gcccaaauac agagaccuag gauugacaga aauaaucgag     2100

agacgagaug gacgcaaagu agaaguuaua aaagauugga cuaaacgaaa cgaccaccgg     2160

caccacgcaa uggaugcacu gacaguggcu uucacaaaac caagcuacau acaauaucua     2220

aaucauuuaa acgcccggaa agacgaaaac aauaaauugu acgcagacau acuaggaauu     2280

gaacgaaagg aaacgaccaa aauacaugac gagaauggaa acgaaaaacg aguuuucaua     2340

gaaccaaucc cgggcuuccg ucaaauagca aaaaaauauc uagaagaaau acuaauaagu     2400

cacaaaacaa aaaauaaagu cgucacaaaa ucaauaaaca aaauagcggg uacgaauaag     2460

aaacaagaga cucuaacacc uaggggacaa cuacacaaag aaacuauuua cggaaaauac     2520

cacuacuauu caaacaaaga agagaaaaua gguacaaaau ucgauaaaga aacaaucuca     2580

aaaguaucca acccaaucua uagauaugca cuacaaaaaa gacuggaagc aaacgaaaac     2640

aacccaaaaa aggcuuuuac aggcaaaaau gcgcuaagua aaaacccaau cuaccuagac     2700

gaugccaaaa caaaaauauu acccgaaaaa guuaaccuaa uauacuuaga agaagacuuc     2760

ucaaucagaa aaaaaauagu aagcgaaaac ccuacaucaa aagaauucgu acccaacagu     2820

auaaguauag agaaaauagu ggaugaagga auaaaaagaa uucuaaagga aagacuugaa     2880

acguacaaca acgauccaaa gaaagcauuc gcagauuuag auaaaaaccc cauuuggcua     2940

aauaaagaaa agggaaucag cauaaaaagg gucacgauaa guggaguaaa aaauacacaa     3000

gcacuacaua gcaaaaaaga ucacuuagga aaaccaauuc uggauaaaga aggacaagaa     3060

aucccaguag auuuuguauc aaccgguaac aaucaucacg uagcaauuua cagagaccaa     3120

aaaggaaauc uccaagaaca gauaguuagc uucuucgaag caguaguaag agcucagcaa     3180

gggcuuccca ucaucgacaa aaccuauaaa caagaagaag gauggcaauu ucuauucaca     3240

augaagcaaa acgaaauguu cguauuccca aaugaaauaa cuggcuuuaa cccauccgaa     3300

auagauuuau uagacccuaa aaauaaaaag uuaauaaguc cuaauuuauu ucgaguacaa     3360

aaaauaucca uuaaagacua cuucuuccgc caccaucucg aaaccaaugu cgaaacaaac     3420

aacauccuga aagauauaac uuggaaacgc gguggccucu cucugcuaaa aggaauuaua     3480

aaaguacgga uaaaucaccu uggaaacaua guaagcauag gagaguauug a              3531


<210>  5192
<211>  3534
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5192
augaauaaag uccaggaaau agaauacaaa aaaauaaaag aaaaauuagc agacagagaa       60

uaucgcaucg gucuggacuu aggcgucgga agcauaggau acgcaguagu auccuuaaaa      120

aaauacgacg auuuaagcua cuuaccagaa gacauaauuu uagccgguuc gagaauauuc      180

gcaucaucaa uaggagcggu agaacguaga gaauucagac uucaacguaa cucacauaga      240

cauagacgag aaaggaugag auuucuaugg aaacuacuag cagaaaaaca guuagcacua      300

caaccaucaa aagaccucga aaaaaaagaa aauagugcag acugugaaac aagccaaaag      360

agauucccaa uaaacauccu aaaaaacgac cccuacauac uaagguacaa agcacucgau      420

gaaaaacuuu cacucuucca acuaggauau guauuauacc auguggcuaa ccacagaggu      480

acagacagca uccguucauu ucuugaagac acggacgaca aaauaaaaga aaauaccaaa      540

accgcaaaau uagccacuga aaccaaaaaa auaauagacu caaaaaaaua ccgaaccuuu      600

ggugaauacc uauauaaaga acguauacaa caaaacgaac gaaaaaaagu aucuaacaga      660

aaaacugcag guaacaaaaa uaaagaauuu gcaguaacga gagacaugau auucaaagaa      720

acagaaaaaa uccuugccag ucaaaaacaa uacuauacga augugcuuac cgacgaguau      780

auaaaagaaa ucgagggugc aauauuuuac caaacggaaa aacucauccc agaaucgggu      840

uacugcccuu auuucaagga cgaaaagagg uuacccugua gucacaaauu aaacgaauac      900

cgaagaauau augaagcacu aaacaacgca agauauuccg caccuguauu agacgaaaaa      960

accggggaaa uuuuaagcua ucaagaaggc gaggaauuuu cgagugaaga aaaaaaaaua     1020

cuauucgaug agguccugcu aaaaggaaaa aaacuuacag aaagcgaaau aaaaaaauua     1080

cuucauuuac cagacggaug cgaaauccaa cuacagggga gagauaaaag aacccaaaaa     1140

auaaaaggau augcgcuuac agcccucgaa gcgcucccau ucuggagcaa acuaucacaa     1200

cagcaacaag augaauuccu cuaugauugg aacggacuac ccgaagaaaa acucaaacca     1260

aaacuaauug acugcuacuc auuaacagaa aaugaaguag acgaaacauu ccaaaagauu     1320

cgacuaccau caaacuacgc cccggucggc aaaaaagcga uggaaaucuu acuaacuuau     1380

aucaaagaug gguacucuua uacugaagcc uuggauaaag cuguaaguga aggaaaauuc     1440

aaaguagaua agcaaacgac uauuuacgau ucguuacccu acuaugguaa aauccuacaa     1500

gaaucaacac aaaaacugau aggaaaggcc uucagcaaac aauuugcaaa acgaaauuau     1560

aaaaaaccaa auacaaaccg aaacgaacga gaauucggaa aaauagcaaa ucccguaguu     1620

caccaaagcc uaaacgaacu acgcaaacuc auaaacgaaa uaauaacagu auucgguaaa     1680

aaaccacugg aaauuggccu agaaaccgcc cgugaauuaa aaaaauccag caaagauaga     1740

gaaucguuag ucaagcaaca aaaugaaaac gaguauaaca gaaacaagau auacaaagau     1800

uacguugagc cacaucuauc acaaauaaaa ucccgccaag aaaacccaag ccgguacaua     1860

uucaaauucg aacuauucga agaacaaaaa ucaauaugcc cauuuugccu aaaagaaaua     1920

acccccgacg auauaaucaa uagcaaagug gacauagaac auauauuccc aauagaagaa     1980

uccgaagacg acgcuaaaaa uaaccuagua auagcacaca augaaugcaa cgcagauaaa     2040

ggaaaaaaaa guccauuuga ugcauucggg gauaaaaccu cagguaaaua cguuuggaac     2100

gauauacuuc acaaugccaa aaaaaaccug ccccauaaga uauggcguuu cuaucaagga     2160

ucuuuagaaa aauucuuaga aaauaaacca augcuaaaaa gauucggaac cgacaauagc     2220

uacauuucua aaauagccgc caaauaccua gcaugucucu uuaacaauuc auccaaaauc     2280

uuuugcauca aaucaucacu aaccgcacaa uuacgaaucg caugggaauu aaacucaauc     2340

augauaccuc uagcaaaaaa cauccuaagc gaaaaggaau uggaagaauu uaaaacgcuc     2400

aaaaacaaaa aaaucagaau ggacaaccgc caccacgcgc uagaugcaau cacaauugca     2460

uacgcaaauc gaggauauca uaauuucuua aauaaaauac augcaaaagg uuacaaaauc     2520

gacuaucgag aaaaaaacug guuaaguaaa auucuccuuc cuccacucaa cagagaccuc     2580

gaagacuuua aaaucuccgu aaaaaauagu auagaaaaag ccaauguuag cauaaaacau     2640

gaucauaaaa cuaauggauc acucgugaaa ggaacugcau auaaaguuua cuauucagga     2700

accgguaaau auauaauuac aacacuaaaa aacgucgaag aaauaucauu uaaagaaaaa     2760

gaagauccug aagacacacu gaggagggca cuauguggau uugaagacuc caauauaaaa     2820

gaugaaaaau uaauaaaaaa gcucaaauac aacucagacc ucuauaagaa aauauuaaac     2880

acaaaaccaa aagcaaaaca agaacuagaa caggcuaacc aagaaacaaa agaggaagga     2940

aaaaaaacac aaaaaaucac cgacguacua auauacaaaa aagccugcag ccuagucgga     3000

gagcaauaca uccaaugugc aucaagacuu gaaaacaaau uuuucgcagu aaaagaacca     3060

acagaaaucc gaaccggaau uggcuacgac accggagaca acuuauuccu agaucuuuau     3120

uacgacaaaa caaggaaguu augcggggag auaaucagaa aaauagauuu uaaucaaaac     3180

aaaauaccga auuaccaaaa agaaggauac auacuauucg aaagaauaua ccaaggagau     3240

acacuugaag cagacacauc agaagacaaa auauccuuaa aaaauaaaac agguuccgca     3300

cuugauaaua gaaccuuugu uaaaguacua acguuuacag agucagacaa auacuucgau     3360

ggaaaaaaag auaaaauaca gaucuucuuc ggcaaccuau uaaaaucaaa cuacaaacaa     3420

gacgacucau ucuauauaag uaguaugcaa aaauacaaug uacgaaaagu aauccuuacg     3480

agcuuaggua uaauaaaaua ccguucacag auacucaaaa acaaggaagu cuga           3534


<210>  5193
<211>  3540
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5193
augagcaaaa aaguuucacg aagguaugaa gaacaagcuc aagaaaucug ucaaagacua       60

ggcucucgac cauauucaau cggccuagau cuaggcguag guucaauagg gguagcggug      120

gcagcauaug aucccauaaa aaaacaacca ucagaucuug uauuuguaag uagucgcauu      180

uucauaccau cgacuggugc agcagaacga cgacaaaaac gcggacaaag aaacagccua      240

agacacagag cgaauagacu uaaauuccua uggaaauuac uagcagaaag aaaucuuaug      300

cuuucuuacu cagaacaaga uguucccgau ccagcaagau uacgcuuuga agacgcagua      360

guacgcgcua aucccuacga acuaagauua aaaggucuaa augagcaauu aacucucuca      420

gaauugggcu augcauuaua ucacauagca aaucaucgcg gaucaucuuc aguaagaacu      480

uuccucgaug aagaaaaauc gagugacgau aagaaauuag aagaacaaca agcuaugacc      540

gaacaauuag ccaaagagaa agguauauca acuuuuaucg aaguuuuaac ugcauucaau      600

acaaauggau uaauaggaua uagaaauagc gaaucaguaa aaucaaaagg uguuccagua      660

ccuacuagag auauaauauc aaacgaaaua gauguacuac uacaaacuca gaaacaauuu      720

uaucaagaaa uacuaucaga cgaauauugu gauagaauug uaagugcaau acuuuuugaa      780

aaugaaaaaa uaguacccga agcugguugu uguccauacu ucccagauga gaaaaaguug      840

ccucgauguc auuuccuaaa ugaagaacga aggcuauggg aagcuauuaa caacgcgaga      900

aucaagaugc cgaugcaaga aggagcugcc aaaagauauc aaucagcaag uuuuucugac      960

gaacaacgac acauacuuuu ucauauagcc agaucaggua cagauauuac gccaaaacua     1020

guacaaaaag aauuucccgc acuuaaaaca aguauaaucg ugcuacaggg uaaagaaaag     1080

gcuauacaaa aaauagcagg uuuuagguuc cguagguuag aagaaaagag uuuuuggaag     1140

aggcuaagug aggagcaaaa agaugauuuu uucuccgcuu ggacuaauac uccagacgac     1200

aaaagauuaa guaaauaucu aaugaaacac cuucuuuuaa cagaaaacga agugguagau     1260

gcacucaaaa caguaagccu uauuggcgau uacggcccca uaggcaaaac agcaacccaa     1320

cuacuaauga aacaccucga ggauggacuc acuuauacug aagcacuuga aagaggaaug     1380

gaaacgggug aauuccaaga auugucugua ugggaacaac aaucauugcu uccguauuau     1440

ggccaaaucc uaacgggauc aacacaagca uuaaugggaa aauauuggca cucagcuuuc     1500

aaagaaaaaa gagauucaga aggcuucuuu aaaccaaaca cuaauucaga cgaagaaaaa     1560

uaugggagaa uugcaaaccc uguaguacac cagacauuaa augaacuaag aaaacuaaug     1620

aaugagcuaa ucacuauacu cggcgcaaaa ccacaagaaa uuacgguaga guuagcacga     1680

gaacuaaaag uaggggcaga gaagcgagaa gauaucauaa aacaacaaac uaaacaagaa     1740

aaagaagcag uccuugcuua cagcaaauac ugugaaccga auaaucucga caagagauau     1800

auagaaaggu uucgacuacu agaagaucaa gcauuugucu guccauacug ucuagaacac     1860

auaagcguug cagauauagc ggcagguaga gcugacguug accauaucuu uccacgggac     1920

gauacagcgg auaacucaua uggaaauaaa guugucgcac aucgacaaug uaacgauaua     1980

aaagguaaaa gaacacccua cgcagcauuu aguaacacaa gugccugggg uccuauaaug     2040

cacuaucuug augaaacacc uggaaugugg cgcaaaaggc gaaaauuuga aacgaaugaa     2100

gaagaauacg caaaauaucu ucaaagcaaa gguuuuguaa gucgauucga aagugauaau     2160

agcuacauag cuaaagcagc aaaggaauau cuaagguguu uauuuaaccc caauaauguc     2220

acagcggucg gaaguuugaa agguauggaa accucaauac uacguaaagc auggaaucua     2280

caaggaauag augauuuacu aggauccaga cauuggagua aagaugcaga uacuagcccu     2340

acaaugagaa aaaacagaga ugauaaucga caucacggcu uagacgcgau aguagccuua     2400

uauuguucca gaucauuagu gcaaaugauu aacacgaugu cagaacaagg gaaaagagca     2460

gucgagauag aagcuaugau uccuauucca ggauacgcau cagaaccaaa ccuaagcuuu     2520

gaagcucaac gcgagcuauu cagaaaaaaa auacuagaau ucauggaucu acacgcauuu     2580

gucucgauga aaaccgauaa ugaugccaau ggagcacuau uaaaagauac cguuuacuca     2640

auauuaggug ccgacacuca gggggaggac uuaguguuug uaguuaagaa gaaaauaaaa     2700

gauauugggg uaaaaauagg agacuaugaa gaaguagcca gugcaauuag gggaagaauc     2760

accgacaaac aaccaaaaug guauccaaug gaaaugaaag auaaaauaga acagcugcag     2820

agcaaaaacg aagcugcacu acaaaaauac aaagaaagcc uaguacaagc agcagcaguc     2880

uuagaagaau caaauagaaa gcuaauagaa ucaggaaaaa aaccuauuca auuaucagaa     2940

aaaaccauaa guaaaaaagc uuuagagcua guaggcggau acuauuacuu aauuaguaau     3000

aacaaaagaa caaaaacauu cguuguaaaa gaaccuucca augaaguaaa aggauucgca     3060

uucgauacgg guuccaacuu auguuuagau uuuuaucacg augcccaagg aaaguuaugu     3120

ggagaaauaa uucguaaaau acaagcaaug aaccccagcu auaaaccagc auauaugaaa     3180

caaggauacu cacuauaugu gagacuauau caaggagaug uaugugaauu gagggcaagc     3240

gaucuaacag aagccgaauc aaaccuggcc aaaacaacac auguuagauu accaaacgca     3300

aaaccaggga ggacauuugu aauaauaauc acguucacag agaugggauc uggguaucaa     3360

auauauuuuu caaacuuagc aaagaguaag aaaggucaag auaccuccuu caccuugacc     3420

accauuaaaa auuaugacgu gagaaaaguc cagcucucau cagcuggccu cguuagauau     3480

guaucuccau uacuuguaga uaaaauagag aaagaugaag uagcucuaug uggugaauga     3540


<210>  5194
<211>  3567
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5194
augaaauaua uauuaggauu agacaucgga gucucauccg uaggaauagc aguaauuucc       60

gaagaaaacc aaaagaaaac uaucgaaaac cuagcaguca gaauuguacc ugaagauccg      120

gauuuccaug guaaauucua uucugguaau acagcaagua aaaaccuuga acgcaccgcu      180

aaaagaggga accguaaaaa caaccaaaga uucaaagcca gaagagauaa auuauaccaa      240

ucauuaaaag aaaacaacau guucccaaca ucaaaccuau uugaccuauc ugcagucgaa      300

cuauauggau uaagagcaaa agcgguaagu gaacaaguau cacuacaaga auuaggaaga      360

auacuaauau uacuaaauca aagaagagga uuucuaagua aucgcaaauc cguaucugaa      420

gaaaacagca cagaauacaa agaacgcauu gcuggauuag aaaaagcauu aggaaauaaa      480

acuauaggac aaaaauuaua uucugaauua caagaaaaca acaauguauu cgagguauua      540

uugcgagaaa gaacauacca aagaucaagu uauacggaag aguucgauag aauaugggau      600

gaacagaaga aauauuaucu aaucuuaaca ggaagcucaa acgaagauga caauaaagga      660

acucuuuaug aucuaauucg aaauaggaua auauacuacc aaagaccauu aaaaucacaa      720

aaaggacuga uuagcgaaug ucuauucgaa aaacaccaca aagccguagc aaaaagcagc      780

ccuuacuucg aacuauuucg aauauggcag aaaaucaacg aucuauccug gaaaacacca      840

aacggagaac uauucaaacc aacagacgaa caaaaacaaa aacucaaaaa ugcccuaugg      900

aauggugaca auuuaaaucc caaacauaaa uuaaccgucu cuaaaaucaa agagauucua      960

ggguacacau caagagagaa aauuuacuua aauuuuacag aacuagacgg uucucguaca     1020

uacucaaucc uaagaaacgc ccuaaucgag gcuggcauag aaaaaccaga acaauaucuc     1080

uucuuuaaca uggaaacaaa ugacgaaaaa gggggccucu ucgaacucug gcauauaacu     1140

uauucacuac caauagaaaa agaaguaaua aaugcacuua aaaauagauu uggauuugac     1200

aagaaacaag cagaaauaau agcaagacgc guagguuaca guagcgauua cggcagccua     1260

uccacaagag caauaagaaa auuauugguc cauuuagaaa aaggacucgg auacaccgac     1320

gcaugcgaua acaucggcua cgaucauuca ggauacaaaa caaaaauuga ucuacaaccc     1380

aaacuuaaac aacuaaaacg aaacgagcuu agaaauccag ucguagagca aauacuaaac     1440

caaguaguaa acgccguaaa cuuaauaaua gaaaaauaug gaaaaauaga cgaaauacgc     1500

guagaauuag ccagagaacu caggaacaac gcaaaaacac ggaaaaacau cucacugcuu     1560

aacagcaaaa auaagaaaaa caacgacaua auaaggaaga aauuacaaga agaauaugga     1620

uuuaaauucg uaaauggaag agacguuaca agauauauac uaugggaaga aaacucgaaa     1680

gaauguuuau acugcaacaa aacaauaaac ggaucagaca uguuaacggg uaaugccgac     1740

auagaacaua uacugcccaa aucaagaagu uucaauaaug cuaugaacaa uuacaucaua     1800

acucauagaa agugcaauuc agacaaggga cagaaaaccg cguacgacuu uaugagcuca     1860

aaaggcgaag uuauauugca acaauacaua gagaaaguaa acgaccuaua caaagauggc     1920

aaagggucaa uaagcaaaac aaaauuugaa aaucuacuau gcaaaggcga agacauuccu     1980

ucagacuucg uagaaagaau gaagaaagau ucgcaauaua uagcaaaaga agcagucaaa     2040

auguugaaaa caguuugucc uaauguauac accacgacgg gacaaguaac ggauuuccuc     2100

agagaagaau ggaaauugaa aaaccuacua caagagcuaa ccuucgaaaa auacaaagca     2160

auaggccaag uuaaagagaa aauaauaaaa acaaauaacg gacaaacgaa aacauuuaaa     2220

gugauagcug auuggaguaa aagagaugau cauagacauc acgccauaga cgcacucaua     2280

ugugcacuaa cagaccagaa aauaauauuu aaacuaaaca accuaaacaa aauauaccaa     2340

uaugaacgaa augcccuaag ucaaaaagaa aucgaagaaa ucgaagaauu ucuggaagga     2400

aaauuuagua uaaaagaauu uagugcucaa caaggaaaca uaauagaaug caguauacca     2460

aauauacgca aagacgcaca aaaacacuua gaaaacauuc ugauaucguu caagaaagaa     2520

aauuccaaag uauugaccau gaacagaaac caacccaaaa auuguaaaga acaaauaacu     2580

uggguaccac gcgcacgacu ccaugaagac accauaaugg ggaggguuaa aagaauccca     2640

gaaaagaaaa ugaaauuaaa ugauaaagua ucauucgaac aaauaaaaau gauaguaaac     2700

ccagaaauca aagaaauacu cuuauacaga cucgcaaaau uuggagacaa cccaaaagug     2760

gcuuuugcaa cuaaagcauu aaagaaagaa cccauauuuu acagggaaaa ugaacucaaa     2820

gaagucucaa uauacgaaug gguuaauaca aaacgugugg cacuuaacga aaacauaacc     2880

gauucccaaa uagagaaaau uauagaccaa gcaucaaaaa gagccguuaa agaaagacuc     2940

aacggcggaa agauaaaaga aguauucaaa aaccuaacag aaaacccaau acaacucaaa     3000

aacggcauga uacucaaaaa uguaaccguu acugaugaaa guaagguaga gaaaauaaga     3060

aacggauucg caauaaccgg caaaaaccau cacgcauuag uauauaaaga ugaaaaugga     3120

aaauauaaug acaaaguagu uucauuuugg gaagcgguag caaucggauu aagaaacgua     3180

acagaguaug gaaacccaua uccaauaaua aacagaaacc auaaugacga acucggagaa     3240

uuuuacuuca gcaugcaaau uaacgaccug uuugucuuug acuugaaaca uucagaaaau     3300

ccacaagaag aaaacgagau aaacuuuuuc gacguagaaa aucgcaaaca aauauccgau     3360

aaauuauuca gaguacagaa aaugagcaaa aagaguaaug gacaauuugu guccgacuuu     3420

agacaucacc ucgaaacauc aguaauuaga aaugauauga uucucaaaaa uauaaccugg     3480

auaaguauaa gcaaaaauuc ggaccucgag cgccucacca aaauuagagu caaccacuua     3540

ggcgaaauca uaaaaguagg agaauga                                         3567


<210>  5195
<211>  3585
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5195
auggacuuca cugcugacgu cuccgaauua aaugaauccu uuaugcagau acauacggaa       60

ucaaaauuug aaauugauac cgcaaaagua caagaaauuu cacaauugcu acuggacaac      120

aaaauaacaa gaauggacag acaaaaacaa gucgcaaacg uccuagcaaa aaacuccggc      180

gacaaggaag cagacaaaau aaauaaaaaa aucgccaccg aauuuugcaa ggcuguacuu      240

gggaacaaag caaaauucaa cguaauauua ggagaagaug cgguagauag ugacugguca      300

uucaaaaugg acgaugaaaa cagcgaugag gaaaucgaaa uccucauggg acaaacuaau      360

ggacaacaac agaauguguu gaggauaaua caccaauggu auucccaaau aacuuuaaau      420

ggaauagugc cagacggaca aaugauaucg gaauccauga ucgacaaaua ugaauugcac      480

agaaagcauu uauuauuacu gaaacaacaa auaaaaguaa caaaagacaa aaaaaaagca      540

cagcaauuac augaagcaua cgaacaauau guccacggaa caggagccaa aaaaaaauua      600

acacaagacg auuucuauuc aaaaguuacc aaaaauuuag aaacaaguga cuucucagac      660

gaaauuaaaa aacaaaucaa ugcagaaaca uuuaugccaa aacagcgaac aucagcaaau      720

ggagugauac cgcaucaacu gcaccaacaa gaauuagaca aaauaauuga aaagcaagcc      780

aaguauuauc cuuuuuuagc agaacuaaac ccugacacca aaaggcaaaa cauagcaaaa      840

uauaaauuag acgaacuaau agcuuucaaa auacccuacu acguaggccc auuaauaaca      900

acagaagauc agaagaaaac aagcgguaaa aguuucgccu ggaugguaag aaaagaacca      960

ggcaaaauaa cuccauggaa cuuugaucaa aaaguagaca aaauggcaag cgcagaccaa     1020

uuuauaagaa gaaugacuac gaaagacuca uaccuuauaa ccgaagacgu acuaccagcg     1080

gaaagucuga uauaucaaaa auauaaaguc cuaaaugaau uaaacaugau aagagucaac     1140

ggaaacaaau uaacaaaaca ugacaaacaa aacauauauc aacaaguuuu cgaaaaauau     1200

aaaacuguua acguaaaaaa aguucaaaau uacuuccuaa cugauggacg auaucaacau     1260

gcuccaaaaa uaaccggauu aagugaugac aacaaauuua auucaaaucu cucgaccuau     1320

auagacuuca aaaaaaucuu ccaagaugua guagacgauc cucaaaaaca aaaagauuuu     1380

gaacgaauaa ucgaaugguc aacaaucuuu gaagauagac acaucuauga aguaaaauua     1440

aaagaaauca guuggcuuaa ugaugagcaa cgaaaagaau uaaagaaaaa aagacaucaa     1500

ggauggggcc gucuaucagc caaacuacua acaggaaucu uugaugaaaa cgguuacuca     1560

auaauggauc aacuauggaa cacgcagaaa aacuucaugc aaauacaagc agaagaagga     1620

uucaacaaaa aaauucaagc agaaaacagc aaacauuuag aaaaaaacga uuucgaggac     1680

auacuugaag augccuacac uucaccucaa aauaaaaaag cuauccggca aguaaucaaa     1740

guaguagaag acauacaaaa aacaaugggg acagcaccuc agaagauauc cauugaauuc     1800

acacggucag ccgauaaaaa cccgcgacga agcauagaaa gacaaagaca auuagaagaa     1860

aucuacuaca augacgcaaa agaaauagua agcgaaggca ugcaagacaa cuuacaaaac     1920

uuuacagaca acaagcagac ccuuacugac aaauacuucc uguauuuuac ccaacuaggu     1980

aucgacaugu acaccggaaa aaaaauaaau aucgaugaaa ucgguaauua ccaaauugau     2040

cacauacuac cacaagccuu cauaaaagac gacucuauag auaauaaagu acucgucagc     2100

ucagcaauaa acaaugggaa aucagacaau guaccacucu caauguaugc auccgucaga     2160

aucaaaucag aagugcaaaa caaauggaag aacuucgcag aacacggccu aauaucccaa     2220

agaaaacuaa auaauuuaau gacagaucca gaaaguauag auaaauauaa ggcacgcggu     2280

uuuauuaaac ggcaacuagu agaaaccuca caaguaauac gacuugucgc cgaaaucuua     2340

aaugagaaau aucaaggaga aacagaaaua auugaaguca gagcaaaaau gaacagccaa     2400

uuacgaaaag acuucgaucu acuuaaaaac agaaauguga augauuacca ucacgccuua     2460

gaugcguacc uaacgacauu caucgguaau uaccucuacc gcagauaccc aaaauugcga     2520

uccuauuuua cuuaugguca uuucaaaaag auagacgacg cuaaauuaaa acaguucaau     2580

uuuuuacacg acauagaaaa ugacaaagac cccaaauacg ugaaugacaa uggugaacua     2640

cucugggacc gggaaaaaga uaucgcguau uuuaaaaaaa uauauaacuu caaauauaug     2700

cuaguaucac augaaguauc aacaagacaa ggagcaaugu uuaaacaaac aauauuccca     2760

gcagccgcgg augccaaauc aaaguugaua ccaauaaaaa acgauaaacc aacagcgaua     2820

uaugguggau auacaaauaa caacgacgca uaucuagcua uaauaaaacu ugcaaaaaca     2880

ggucaauuua aaguagucgg aguuccacag cgagcaauag ucaaacuuaa acaggccuau     2940

gacaaagaaa cuggcgauua uucaaacguu cuuuauaaag uacuggaacc aaaauuuguu     3000

aaaaaaaaag uaaacagaaa aacuggagaa aucacauacc aaguugaaaa auuuaaaaua     3060

guagucccga aagugccaua cagacagcua guuauagacg gcgaucaaaa auucaugcuu     3120

ggauccagca cauauaaaua caacgcuaaa caacuuguac uaucugaaaa ugccaucaaa     3180

auauuagaca aaaaaagcuu cgaucaaucu ggauauaaau caagcgaccu cgauaccgua     3240

uacgacgaaa uacuaagcca agucgauaga uacuuuucaa uguaugauaa aaacaaauuc     3300

agagaagguc uccauaaugg uaaaaaauau uuccaacgau ugcccaugau agauaaugua     3360

gaaaagggca aaauaaaaga aaauggaaaa uuuaagcucu ugaaugaaau acuaauugga     3420

uuacaugcaa aucccuccuu uggaaauuua aaaagccuag gcauaucuac acccuucgga     3480

augaugcaau ucgcuucagg aauaagcuua ucggaaaaug ccauccuaug cuaccaaagc     3540

ccgacuggca uauucgaacg cagaguugca uuaaaagauc uauga                     3585


<210>  5196
<211>  3588
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5196
augcaaaaga auauaaacac aaaacaaaau cacauauaua uuaaacaagc acagaaaauc       60

aaagaaaaac uaggugauaa acccuaucga auaggacuag acuuaggcgu agguucgauc      120

ggauuugcaa uaguaaguau ggaagaaaau gacggaaaug uccuauuacc aaaagaaauc      180

auuaugguag gaaguagaau cuucaaagca agugcaggag ccgcagauag aaaacuuucu      240

cgaggacaaa gaaacaauca uaggcacacc cgagaacgga ugcgcuaucu auggaaaguc      300

uuggccgaac aaaaacuagc acuaccugug ccugcagacc ucgacagaaa agaaaauucu      360

uccgagggag aaacaagcgc aaaaagauuc cuaggagacg uguuacaaaa agacauauac      420

gagcuacgag uuaaaucucu agacgagcga uuaagucuac aagagcuggg guacguguua      480

uaccauauag caggucacag aggaucuagc gcaauacgua cauuugaaaa cgacucugaa      540

gaagcacaaa aagaaaauac cgaaaacaaa aagauugcag gaaauaucaa aagacuaaug      600

gcgaagaaaa acuacaggac cuacggugaa uaccuauaua aagaauuuuu cgaaaacaaa      660

gaaaaacaca aaagagagaa aauaucgaac gcagcaaaua accauaaauu uagcccaaca      720

agagacuuag ucauaaaaga agcggaagca auacuuaaga aacaagcagg aaaagaugga      780

uuucacaaag aauuaaccga agaauacaua gaaaaacuca ccaaagcuau aggauaugaa      840

uccgaaaaac ucaucccgga aagcggauuu ugccccuauc uuaaagacga gaaaagacua      900

ccugcaucuc acaaguuaaa ugaagaaaga agacuauggg aaacauuaaa caacgcaagg      960

uacucagauc ccaucguuga cauagucaca ggagaaauca caggauauua ugaaaaacaa     1020

uuuacuaaag aacaaaaaca aaaauuauuc gacuaccuuu uaacagguag ugaacuaacg     1080

ccggcucaaa caaagaaauu acuagguuug aaaaauacaa acuucgaaga cauaauauua     1140

caagguagag auaagaaagc acagaaaaua aaaggauaca aacuaauuaa auuagaaagu     1200

augccuuucu gggcaagacu auccgaagcg caacaagauu cauuccuaua cgacuggaac     1260

ucauguccag acgaaaaacu acuaacagaa aaacuaagua augaauauca ccuaacagaa     1320

gaggaaaucg auaaugcauu caacgaaaua guacuaagua guucauaugc uccauuagga     1380

aaaucagcaa uguuaauuau cuuagagaaa auaaagaaug acuuauccua uacugaagca     1440

guagaagaag cacuaaaaga aggaaaacua acuaaagaaa aacaagcaau aaaagauaga     1500

cucccauacu acggcgcagu auuacaagaa aguacucaga aaauuauagc aaaaggauuc     1560

aguccacaau uuaaagauaa ggguuauaaa acgccacaua caaauaagua ugaauuggaa     1620

uacggaagaa uagcaaaucc aguuguacau caaacgcuua acgaauuacg caaacuugua     1680

aacgaaauaa uagacauccu ggguaaaaag ccuugcgaaa ucggauuaga aaccgcacga     1740

gaacuaaaga aaagcgcaga agauagaagu aaacugucaa gagaacaaaa cgacaacgaa     1800

ucaaauagaa acagaauaua ugaaauuuau auacgaccgc aacaacaagu aauaauaacu     1860

agaagagaaa acccaagaaa cuauauccuu aaauucgagc uauuagaaga acaaaaaucc     1920

caauguccuu uuuguggcgg acaaauaagu ccaaaugaca uuauaaauaa ccaagccgac     1980

auagaacacc uauucccaau ugcggaaucu gaagacaaug gaagaaacaa cuuagugaua     2040

ucccauucag cgugcaaugc ugauaaagca aaaagaagcc ccugggcagc auucgccuca     2100

gccgcgaaag auagcaaaua ugacuauaac cgaauauuaa guaauguaaa ggaaaacauc     2160

ccacacaaag ccuggagauu caaucaagga gcauuugaaa aauucauuga aaauaaaccg     2220

auggcggcac gauuuaaaac agacaauagc uacauuagca aagucgcaca caaauaucua     2280

gccugccuau ucgagaaacc gaauauaaua uguguaaaag ggucucuaac ggcucaacuu     2340

cggauggcuu ggggacuaca aggccugaug auaccuuucg caaaacaacu cauaacagaa     2400

aaagaaucag aaucauuuaa uaaggacgua aacucaaaua agaaaaucag acuugauaau     2460

agacaucacg cacucgacgc aauagucauc gccuaugcau cacgaggaua cggaaaucuu     2520

cucaauaaaa uggcgggcaa agauuacaaa auaaacuacu cagaacggaa cuggcuaagu     2580

aaaauccuuc uaccuccaaa caauauaguu ugggaaaaca uagacgcaga ccuggagagc     2640

uuugaaagua guguaaaaac agcacucaaa aacgccuuua uaaguguaaa acacgaucac     2700

uccgauaaug gcgagcuugu aaaaggaaca auguacaaaa uauucuacuc cgaacgaggu     2760

uacacauuaa cgacuuacaa gaaacuauca gcauuaaaau uaacagaccc gcagaaaaag     2820

aaaacaccaa aagauuuucu agaaacagcg uuguuaaaau uuaaaggucg cgagucugaa     2880

augaaaaacg agaaaauuaa aagcgcaaua gaaaauaaca aacgauuauu ugauguaaua     2940

caagacaacu uagaaaaagc aaagaaauua cuggaggaag aaaaugagaa aagcaaagca     3000

gaaggaaaga aagagaaaaa uauaaaugac gcaucaaucu accaaaaagc uauaagccuc     3060

ucuggagaca aauacguuca acuaaguaag aaagaacccg gcaaauucuu ugccaucuca     3120

aaaccaacuc caaccacuac aggauaugga uaugauaccg gagauucacu cugcgucgac     3180

cuauauuacg acaacaaagg caaacuuugc ggagaaauaa uucgaaaaau agaugcucaa     3240

cagaaaaacc cacugaaaua caaagaacaa ggauuuaccc uauuugagcg aauauauggg     3300

ggcgacauac uugaaguaga uuuugauaua cauagugaca aaaacuccuu cagaaacaau     3360

acuggaagcg ccccagaaaa cagagucuuu auaaaaguag guacauucac agaaauuacc     3420

aauaacaaca uacaaauaug guuugguaac auaauaaaaa gcaccggugg acaagacgac     3480

aguuucacga uaaacucaau gcagcaauac aauccaagaa aauuaauacu uagcucaugu     3540

ggauuuauaa aauauagguc cccaauauua aagaacaaag aaggauga                  3588


<210>  5197
<211>  3594
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5197
augaguaaac augucuuagg acuagaccua ggaguaggau caauaggaug gugucuuaua       60

gcauuagacg aacaagauca accggcugaa aucuuaggaa uggggucaag aauugucccc      120

cugacgacag augaccuuaa ugaguucucc caaggaaaag caaucagcaa aaaccaaaaa      180

cgcacaacac aaaggacuau aagaaaaggc cuagaccgau accaauuaag aaggaaagua      240

cuuuccgaaa aacuagcagg gcauggcaug cuuccagaca aagaucucau gcaguugcca      300

guauuagacc uaugggaauu acgugcaaga gccgcuagcc caggagaaaa auuaucauug      360

ccagaaauag guaggguacu auaccacauu aaucagaaaa ggggcuauaa acacgcuaaa      420

ucagaugaag cagaagaagu aacuaaacga ggcaaagaac aaggcuaugu agcaacaguc      480

aaagggagau acgcugaacu acaaagagaa ggagcgacgg uaggucaaca cuuugcaugc      540

aaauuaaaag aacacagaca agaaaguccu ucuggcggau ucuuuuauaa uuauaggauc      600

aaagaucaag uuuacccccg ugaagcauau auugaagaau uugacaggau aauggaagua      660

caaaguguau uuuauccaga agugcuaacc ccugcuuuua uacaagaacu acgagauaaa      720

gucauauucu uccaaaggcc ccuaaaaucu uguaaaggau uaguaaguuu cugugaauuu      780

gaaaaacaag agagaguggu acaauuaaaa agaguuaaua gagaagguaa agaagaauug      840

caagaaagaa aaauuacaau ugguccaaag guagcaccga aaaguucacc cauagcacaa      900

cucuguaaau uaugggaauc aauacauaac auaagaauuu auuauccaga cggcaaagaa      960

aggccgauua cacuagagga aaagagaaaa uuacuagcgu acuuacaaac acaugauaag     1020

uuaacauuca cagcauuaaa gaaagaauua aaaauaagcg acaaagaaag auuguggugu     1080

gauaaauuac ucaaaggagg aauaaaagga gauacuacga gaguuuuacu aagaaaagca     1140

uuagcuggau acaagagaua cgaacccuua cuucggaugg acuuagagcu cagagacuuc     1200

caagagauag ucucagguaa acgauugauu agaccaaucg uagacgaauc guaccuuagc     1260

cagccucugu auagacuaug gcauauccua uauagcaucg aggaaagaga agcuaugcgc     1320

agagcucuca uugaccaauu aggauuucuu gaagaagauu uagacggagg acuacuugac     1380

aaauuauauc aaauugacuu cgcaaagcag ggauacagca acaagagugc caaauuuauu     1440

uguaaacuuc uaccccaccu acaagguggc cacggguauu cucaagcaug cgaauuagca     1500

ggaaaaagac acucagcagg aaguuugaca aaagaagagu uagaagaaag accccuguua     1560

gaacauauac cauuauuaca auguaaugaa cuuagacaac caauaguaga gaaaauuuua     1620

aaccaaauga ucaauuuggu aaaugcacua cgccagaaau auggcaguau ugaugaagua     1680

aggguagaac ucgcaagaga auugaaaaug ucgagggaag aaagagaaga aacaaguaaa     1740

aggaucagaa gcagagaaaa ggaaaacaaa gaaauagcag aaagaauuaa agaauacgaa     1800

cuauacccaa cuaaaucacg cauucaaaaa uauagacuau ggcaggaagc cggagaacaa     1860

uguuuauauu gcggaagauc aauugaugua guacaauuuc uaagagguga uggaguugaa     1920

auagaacaua uuaucccaaa aucaauacua uaugacgacu cuuccgcuaa uaaaaccugc     1980

gcuuguaguc ggugcaauaa agauaaaggu aacagaaccg ccuaugaaua uauucuagca     2040

gaggguagag aagccgaaua cuuagaccga cuugaaacgu uguuccacga cggaaaacua     2100

aguuauucga aaaggagacg acuucaaugg aagaaagcag acaucccagu ugacuucaua     2160

gaaagacaac ucagauuaac acaauacaua gcucgucaag cacaagaaau cuuaagacaa     2220

ggaauacgaa gaguaggugc uagcgaagga agugucacag caaaacugag acauuuaugg     2280

gguuacgacg auauuuuaag ggagcuaaac uucgaacggu accaaaguau gggagagacg     2340

gaaaucguau cucaggaaga gaaagauggu ucuauuacaa aaagugagag aauuaaagac     2400

uggucaaaaa ggaaggacca uagacaccau gcaaucgaug cuuugguagu agcuuguacc     2460

agacaagggu auauacaaag acuaaauagc cuuaacucag aagaagacag uuuacaaaug     2520

caagaagaua gcaaacaaau gagagagaaa augggcggcc aaaagagacc aacggcagau     2580

aaacauucga uauuagaaaa cuggcuaaua cuucaaccac acuucaguca ucaagaagua     2640

aaaagcaaag ucgccgaaau auuagucagu uuuagacccg ggaaaagaac aguauccaca     2700

ggcagaaaua cauaccuacg aucuggaaag aaaguaacac agagcggggu auuaauaccc     2760

agaggacccu uauccgaaga aagccuauau ggacaaauac uuaggaaugg cgaaccaacc     2820

uauguaaaga aaguagcacu agaaucguug uccgaagcac aaauagaaca aauaguggac     2880

ccaaaacuac gcagagaaau gguagcucgu acacagccaa gugagggagc uaaauuaaau     2940

ccaaaauuac uacuugcuac accauucuac agcgacgcgg cagguacaca acguguacgc     3000

aguguaaggg ucgcaauaga gaacggcaaa aacucuauag uaccacuacg guauaacgaa     3060

ucaggaaaag cuauaacauu cguuaacccu ggaaauaauc accauguugg aaucuacaga     3120

acgucugaag gcaaauuagu agaauccugc gucacuuucu ggcaggcagu agaucgagua     3180

cgcuauggaa uaccaacaau aauucgcaaa ccuagagaag uauuagauca acuucuucuc     3240

agagacgaca uacccgaagc auuauuagca caucuaccag cgucagacug ggaguucguu     3300

gaauacuuac aagcuaacga aauguuuuua auaggucuaa cugacgaaga aauacaaaca     3360

gcauuagcgg aaucggauuu auuaacauua uccaaucacc uauaucgcgu ucagaaaaug     3420

ucuagcgaau acuauguauu ucgguaucau cuagaaacag aaguagcaga caacaaaaau     3480

aauacaggua uaauuccaaa auucuacaga cuccgagggc caaaugauac auaucuaaaa     3540

gcuaauccaa gaaaaguaaa aguuaaucuu uuaggagaaa uaagccuacc uuga           3594


<210>  5198
<211>  3615
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5198
auggaaagaa uacuaggaau agauaccggc acaaacucac uaggaugggc aauaguugau       60

aaagacgaaa aucaauauca ccuuguagca aaagguacaa auauauucag ugaaggagua      120

aagauugaaa aaggcauaga auccagcaaa gcagcagaac gaacagaaca uagaucaguu      180

aggaaacauu auuggagacg uaaaauacga aaaauaagac ucuuaacaau acuuucagau      240

aacgaucuau guccaagacu aaaacacgaa caacuacguc aauggagauu gaaaaaggua      300

uacccaacgg acgacacauu uauggaaugg cagagaacag aggacaaggc gcaaguaaau      360

ccauauaaau ucagauauau uuguuuaaca caaaaacuag aucuauccga caucacacaa      420

cguuuuaucc ucggucgagc acuuuaccau cuaaaucaaa gacggggcuu ccuaucaaac      480

agaaaagaag cuacaaaaga aagugaaggu acaguaaaag agggaauauc aucccuguca      540

aaagauauga aggacucagg augcgaauau cugggagagu auuucuacca auuauaucaa      600

caaggcaaga agauaagaaa ccacuacaca gcacguaaag aacacuaccu aaaagaguuc      660

aaagcaaucu gcucaaaaca agaauuagac gacgcauuaa ucgaaaagcu ggaaaaagcc      720

auauucgacc aaagacccuu aaaaucccaa aaaggacagg uagggacaug cacauucgaa      780

aaagguaaga gccgaugccc aagcagccac ccacucuaug aagacuuuag aauguacuca      840

uuuaucaaua acaucaaagu acagguugau acccuauaug acaaaggaga acgaucauug      900

acagaugagg aaaaacaauu aauaauaccc uuauucaaaa gaaaaucuaa acgcacguuu      960

ccauucgagg auauagcuaa gaaaauagcu gguaaaaaca acuacugcua uauaaaauca     1020

aacgaggaaa aggcuuauaa auuuaacuac caaauggaca cccaaguaag uggaagcccu     1080

gucaacgcac aauuagaaga gauauucgga gaagauuggc uuuccuccgu uugcgaggug     1140

uacacuuugg caaacaauaa aaguagauuc caaauaauga acgauguaug gcacguacuu     1200

uucuuuuacg acgacgaaga caaacuaaaa gaguucgcaa aagaccgucu ucaacuagac     1260

gaugaauuag cagaaaaauu cucaaaaauc aguauuccca augauuaugc ugcauuaagc     1320

cuaaaagcaa uacggaaaau aaugccauau uuaagagacu auggauuaau uuauucagaa     1380

gcaguauuuc ucgcaaauuu aguagaugua cuaccacaac auguaugggg aaucaaagaa     1440

augcgagaag cggcaauaga aaaugugaua gauguuaugc acagcuauga caaaaacaau     1500

auagacggaa uaacuuuaga aggaugcguu aaaacauucc ucaaagaaag auaccaagua     1560

ucagaugaca accuaaagaa acuguaucau ccaagcaugu uagauccaua cccgaggcaa     1620

cgaccuaacg acgaaggagu auuccaauua gguaguccac gcauaagcuc agucaaaaac     1680

ccaauggcaa ugcacucauu auuccggcua cggaaaguag uaaaccuuau gcuuaagaaa     1740

gguuauauaa cccccgacac uaaaguacac auagaguucu cacgcgacuu aaaugaugcc     1800

aauaaaaggu gggcaaucca gacauggcaa agagaaaaug aaaaagaaag agaaagaugc     1860

agaaaagaaa uagaaaaaua cauagcaucc ccaucagaua cagacauauu aaaauaucaa     1920

cuaugggaag aacaaaauca uaaaugucua uacacaggag acgaaauuag aaucaccgac     1980

uucuuaggag auaauccuaa auaugauaua gagcacacca ucccaagauc agccggaggc     2040

gauagcacaa aaaugaaccu uacacuaugc ucaagcaggu uuaacagaga ugaaaagaaa     2100

acaaaacuac cagcagaacu uguuaaucac gacgaaauac uagaaagaau cuccaauugg     2160

aaagaaaaau acgaagauau agauaaacaa aucagaaaaa uaagcacaaa aggugcaaca     2220

ucgaaagagg acaaagacuc aaaaauaaga agaagacacu acuuaauacu acaaagagac     2280

uauuggagag gcaaauacca aagauuuacu augacagaag uaccagaagg uuucucacga     2340

agacaaggaa cagauaucag uguaauuagu cguuaugcac guauguaccu aaaaucugua     2400

uucaaacaag ucuacaucgu aaaaggaaua gcaaccucag auuucagaaa aauguggggc     2460

auccaagaug aguaugccaa aaaggaacga guuaaucaug uacaucacug uaucgaugca     2520

auaacaauag ccuguaucgg aaaagcggaa uacgauaaau uagcggacua ccgccaucaa     2580

uacgacgauu aucucuucca aggaaaaacu aaaccaaccu ucgauaaacc auggccuuca     2640

uuugugauag acaucaaaca uauacaagac gaacuacuua uagcacauua cgcaaaagac     2700

aaucucgaaa aauucaccaa gaaaaauuua agaggcaaag acgguagaau aaucagagac     2760

aaauuuggaa acaaauuaca aaugaaaggu gacacaucua gaaacagccu ccacaacgac     2820

aaauucuaug gugcgauuaa ccacgaaggu gaaauaaaau auguauaccg aacuaauaua     2880

gaagccuuag augccaaaag cauaggaaac auuguggacc caacaguuag aagcaucaua     2940

gaaucagcaa uugccauaca uggcagucua aaagcagcaa ucaauaaagg aauauggaug     3000

aaugaagaaa aacauauacc aauaaagaaa guacgcgucu uccgaaauga cguaaagaaa     3060

ccuauagagu uaaaagacca aagagaucuu ucagauaaag acuauaaaca acacuacuau     3120

guuacuaaug acggaaauua caugaugcua auauucagca aauuaaauaa uaaaggaaga     3180

gaaauaagac aacauguagu aaucaacagu uuagaagcaa cugaaguaau caaaagccau     3240

aaaagaagcc aagucggaaa aguaguaugg gaaucauacu acaguaaaga aggcuucgau     3300

cuaauuuaca aauuaaaaag ugggacuaug auauuauuau augaaaacaa uagagaugaa     3360

guuugggacc uagauauaag aaacuuacag aaaaggcucu acaucauaac gaagaugaaa     3420

uacgauggga gaauauacau gaugcaccau caagaagcca gagagaaaaa ggaccucgaa     3480

gaaaagucag gcucauucaa aauaaaugaa gauuacaggc cacuuaucgu aaacagcuac     3540

aaccaauuca acgccuuagu gcaagaucuc gauuucgaaa ucaaugaccu uggagagauc     3600

agacgucuga uauga                                                      3615


<210>  5199
<211>  3630
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5199
augaagaaag acuacauacu cgguuuagau auagguauau caucaguagg auggggauug       60

uuagaacugg augacaauaa uaaccccuac aaaauaauag acguaggaag uagaauuuuu      120

acuccaggag aagucgagaa aaccggcgau ucaagagcaa aagaaagaag agaaaaacga      180

ggagcucgac gaaucgcacg aagaagagag uucaggcuug acagagucaa aaaccuacuc      240

uaugaaaaug gauaccuaaa aggaaacaua aacucaaaua uagucuccga gaaaaacgaa      300

gaacuaucaa uaauauuuaa uaacaugauu aauaacuacu acaauggcaa aaacaccaau      360

ccauauauuu uaaaaguuga agcacuugac cgaaaauuaa cagacgaaga acuaucuaua      420

aucuuaguac auuacgcaaa gaaacgaggc uauaaaagua acagagaaga agcaagugau      480

aaagaaagug gaaaaguacu aacggcaaua aaagaaaaug agaaaauaau gaaagacaac      540

aacuaccgca ccauuucaga aauguauaua aaagacgaua aauucaaaaa caaaaucaaa      600

aacagcccaa augacuauaa aauuuccgua acaaaugaaa uguauuuaga agaaaucaau      660

aaaguacucg acagccaaau aaaauucggc cucauaacag acgauuucaa aaaccaauau      720

cuagaaauau acaacucaag aagauccuac gcuaaaggcc cuggaggaaa cuccccuuau      780

ggaggagauc ucauugaaag aaugacagga aaauguaacu ucgacggaaa uccgagggcc      840

ccuaaaagag cuuucagcag ugaaauauac guugcauuaa caaaauuggu aaacuuaaaa      900

uacaaaguca acgacggaga auauaucaaa cucucagaaa aagaaaucaa aaacauaauu      960

gaaaaagcga aagagaagaa aaguauaucg uacaaagacc ucacaaaaau gauaggcaau     1020

aacaucgauu ucaaagaccu aacauuaucc aagaaagaau acauaaaauu aaucgaagaa     1080

cuuaaaaaga aacuuaauau caagaaagau acaaaaauca acauaaacga acuaaguucg     1140

gaagauaaag aauuguauaa caaauuaugu aacgacaaau uacuaucaaa gacauugaua     1200

gaacuaaaag gauaccacau acuaaaagac uccguuauaa aagcauauag caaagacaua     1260

ugggaugaag ucaaagacaa uauguaccua uuagacgauc uagcacuaua uugcacaaac     1320

uauaaaguaa acgaagauau cuuaaacaag aucaaagaaa gugaaaacau agauaacauu     1380

uucaaugaca uacacuuugu agaacuacua ccaaauuuca aagaucaucu aauguuauca     1440

acagaacuaa ucagaaaacu aaucccaauu auguacggag gaaacacaua cgauaaagcc     1500

augaacauac uggguuacaa ccauucagac auaaauggcg auaaagaaaa gaaagacuua     1560

uuaguaccua ucuguguaga cgauaauauc accaaucaac ggguaauacg aagccuaaca     1620

caaacccgca aaguaauaaa cagcauaauu aagaaauaug ggaugccgaa aauaauaaac     1680

auagaaacug caagagaauu ggcaaaguca agacaagaac gcaacgaaau ugccaaaaac     1740

aauguagaaa aacaagcaga aaacgaaaag auuaaaaacc aacuuguaga auuaggacua     1800

uuuguaagca ccgacaaaau aucaaguaca gauuuauuga aauauaaacu cuggaaagaa     1860

caaaaugaau uuugcgggua uucaaugaag aaaauaucaa uagaagaccu auuuggaaac     1920

aacaucguac aaaucgauca uauucuaccg uacagccgaa cguauaauga caacuaucua     1980

aauaaaacau uaguauacac aaaagaaaau caagaaaaag gcaacagaac cccauaugaa     2040

ugguucggga aaacagauaa augggacgaa uaugaaacuu acauaaauaa ccucacaaua     2100

ucacaaaaga agaaagacaa cuaccuccua aagaaacuag auuuugauac cgaaagagaa     2160

augcgaagcc aaaaucuaaa cgacacaaaa uauaucagua aagaacuauc cucacuaauc     2220

aaagcauacc uaaacgucga aaaaguaaac auguaucccg gugcaaucac ugcaaaacuc     2280

cgagcccggu ggggauuuaa caggcuuaca cauucguacc uaagcaaaaa cuacauacuu     2340

ccgaaaaaca uaaaagaaga cauaaauaag gauagagaca accaccuaca ccacgccaug     2400

gaugccuuag uaaucgcguc aauaacuccc ucauuacagc agaaaauuac ccuauacgaa     2460

aaauucucac gcuauauaga ugggcuuaca gaacuucaga ucaaaaacau aucauacaua     2520

aagaucgaag auagcgaagg acaauacuau aacgacgaca ccgacgaagu cgaaacgaua     2580

gacaucagag aauauauaaa acaacaauua gccgaaaaca acaucuauua uaacaggcau     2640

aacauaucca aacuggaguu cccauugcca uaugaaaacu ucgauaaaga agcaaaacuu     2700

agaguauaug aacaagaccu agacacacua aaauggaauc uaaaaucaua uaauacauau     2760

acacaaaaag auauugauaa uguucacaca cuaauaccau caauagcuaa aacaaaagua     2820

uccggaaaga ugcaugaaga aaccuacuau ggaauaaaag aaauaggaaa agacgaagau     2880

aagaaaaucu auaaaacccu cagaacaccc auugaaaaag uaaaaagaaa agaccucgau     2940

aauauaccag accgcuuggg ugguucuaaa gauguauacg aaucauuagu cgaaugguuu     3000

ggugacuacg aaguugggga agaaguacua aaagcgaacg gaaauaaaua uccaguuagc     3060

aaaucagaca aagaacacaa agaaaucaag aaacuaaaac uuuacucuga auauaaaaac     3120

acugggcaua ugauaaacaa gucaaaugua gaaaaaggcg gaauauacag aauagacgug     3180

uucaaaagca aagacaaaga agacgacaaa cucuacuucg cagcauacga uauacuugaa     3240

auuaagaaaa ucaauacucu uaaagagaaa aauaggguag acgacaacuu caacaucaaa     3300

cuagacuacg gacaagguaa aaauucaaaa auaaugucgu auacagauau caucaacaac     3360

uaugaucuau acauaagccu caacaaaaac gaccuaauca aaauaacaac caacgacgga     3420

aaagaaagug uagcauacgu uguuggaugc aguagcggaa ugcuagaagu aaaauccaaa     3480

uuaggcgacg gauacgauau aaucaaaaca gaucuuaacg aaaacaacau uuucuccaag     3540

caaagagcgc aauaccaaau uacaguuuca accaucaaag aaauaaagaa auuauccauu     3600

agcguacugg gagaaauauc cggccuauga                                      3630


<210>  5200
<211>  3633
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5200
augagaacaa uauuaggauu agaccuagga acuaacucaa ucggaugggc gguaauaaau       60

uccguuauua aagaacaaac agaaagaaua uggauagaga uggcaggauc cagaauuaua      120

ccaauggacg cagcaauauu aggugacuuu gacaagggaa auuccauaag ccaaacagca      180

gaaagaacac gauuuagagg uguaagaaga cuaagagaaa gacaauuacu aagacgagag      240

agacuccaca aaguucugaa aauacucgga uuucuaccug aacacuacuu aaaaggaauu      300

gacuucgaga aacauacugg aaaauuccua ucgggaagcg agccaaaacu accaugggua      360

aaagacagau ggggcaaaua uaguuuuuug uuucaaacag cuuucaacga aaugcuagca      420

gauuucgcaa aacaucaacc aagccuaguu uucgauggua agaaaauacc cuaugacugg      480

acuguguauu accuaagaaa aaaagcacug acggaaauga ucagcaagga agaacuggca      540

uggauacugu ugaacuucaa ccaaaaaaga ggauacuauc aacucagagg agaagaagaa      600

gaagacaaug cagguaaauc aguggaauuu uacgcacuua aaguccuauc aguagaagcu      660

acagacgaaa agaaaggaag agacauaugg uacaauguac acuuggaaaa cggaugggua      720

uacaggcgaa guagcaaugu uccauuagau ugggaaggac gaguaaaaga auuuauagua      780

acaacagacc uuaauccuga cggaacaccc aaacuagaua aauaugguga aguaaaaaga      840

ucauuucgag cgccuaaaga ggaugauugg augcuuauua aaaaaagaac agaagcagac      900

auaacaggua cacacaaaac gauaggaucu uacaucuacg acgcgcuguu acaagagccu      960

caacagaaaa uaauaggcaa acuaguaaaa acaguggaac gaaaauucua caaggaagaa     1020

cucaacuuca ucuuacaaaa gcagugugca uuucacgcag aacuacaaga uagggaucuc     1080

uacaugcgau guauagaagc uuuauaucca ggaaacgaag uacaaagacg aaauaucgcc     1140

aaccgcgacu uuauauauuu acuaauugac gacguuuugu uuuaccaaag accacuaaaa     1200

acaaaaaaau cauuaauagc aaauugcccu uacgaagaaa acgaauacgu caaucggaaa     1260

acaggcgaaa uaaaaacagc cccucuaaaa uguauagcua aaagucaccc acuguuccaa     1320

gaauuucgcu uaugguauuu uuuaucgaac auaagaguau accaaaaaga aagagaagua     1380

aaugguacau uacaccuuga uguugacgua acgacagaau ucuuaaaaac agaggaugac     1440

uacguagcac uauuugauug gcuaaacucc cgaaaagaga uugaucaaaa agccuuccua     1500

agguacccug cauuuggacu aaaaaaagaa auaaauaacu auagguggaa cuauguagaa     1560

gauaaauucu acccaugcaa cgaaacaaga caugcuauac ugagcagacu cgaaaaagcc     1620

ggaauuggaa uugauuuuuu gucugaugaa aaagaaacag ccuuauggca cauccuauac     1680

ucuguaucug acaagcaaga aauagaaaaa gcacuagcaa cauuugccgu aaaaaacggc     1740

uuaaacgaau cauucguaga gguuuucaaa aaauucccac cauucaagag cgaauacgga     1800

gccuacagcg caaaagcaau aaaaaaauua cuaccauuga ugagaugcgg gaaauacugg     1860

gauaugucac uaauagacgg aacaacgaaa gaaagaauag aaaggaucau agcuggggaa     1920

ugugacgaaa guauuggcgc aaaaguuaga gaaaaagcaa ugaauuuauc cgacauaucc     1980

uguuuuaaac gucuaccacu uuggcuagca ugcuaugucg uauacaauag acauucagaa     2040

gauaaggaaa ucacaaaaug ggaaacaccg gcagacauug augcauacuu agcagccuuc     2100

aaacaacaua gcuuacguaa uccaauaguc gaacaaguaa uuacugaaac acucagagua     2160

gugagagaua uauggaaaca agugggaaaa auagaugaga uacgaauaga acuaggacgu     2220

gaaaugaaaa auccggcaga caaaagaaaa agaaugacua cucaaauacu agaaaacgaa     2280

aaugcaaauu uaagaauaaa agcucuauua gcagaauuug ucaacccaga auacggugug     2340

gagaacguua gaccauacuc cccaagucaa caagaaauuc uaagaauaua ugaagaugca     2400

guauuaaaag gugaagaaca aauaccagaa gacaucgacg uuauccuaaa aaaauuuaac     2460

acuagcaaac ucccaacgaa auccgaauuu cuccgauaca aauuaugguu agaacaaaaa     2520

uaccguaguc ccuacaccgg agaauuaaua ccgcuugcaa aguuauucac agcugcauac     2580

gaaauugaac auguaauucc gcaaucaaga uauuuugaug auucauuuag uaacaaaguc     2640

auaugcgaau cagcagucaa caagcugaaa gauaaucaau uaggauauga auuuaucaag     2700

aaucaccacg gacagaaagu ugagguaggu uuugggaaaa cuguugaaau uuuuucagua     2760

gacucauacg aaagauucgu aaaggagcaa uacgcaaaaa guggaauaaa aaugaaaaaa     2820

cuucuaaugg acgauauacc ggaacaauuc auugaacgcc aacuaaacga cagcagauau     2880

auaucaaaag ucguaaaagg acuacucucc aacauaguac gagaaaaaaa cgacagcgga     2940

gaauaugaac cugaagcagu aucaaaaaau auauuaguau gcaccgguuc aguaacagac     3000

agacuaaaaa aagacugggg aaugaacgac guauggaauu cgauaguuuc accuagauuu     3060

gaacgccuaa augccuuaac ugaaacacaa uguuuugguc auugggaaaa uaaagacggc     3120

aaaaaaguau uucaaacagc aguaccauua gaguaccaaa aagguuucuc gaaaaaaaga     3180

auugaucacc gacaccaugc aauggaugca aucguuaucg caugugcaac aaggaaucau     3240

guaaacuacu uaaacaacga aucggcauca agaaacgcaa aaauauccag auaugaucua     3300

caaagacuau uaugugacaa aaguagagga gaugguaacg gaaacuauag guggauaaua     3360

aaaaaaccau ggacuacauu uacgcaagau gcaagagaag cucuaucagg aauaaucguc     3420

agucuuaaac aaaaccuacg caucaucaac aagacaacca auaucuauca acacuuugau     3480

gcaaacggaa cacgagucua cgagaaacaa aaaauaggag acucaugggc uauacgaaaa     3540

cccaugcaca aagauacagu uuucggcgcg gucaauuuaa ggaaagcguu aauaccgcaa     3600

cauuacaacu uaacucuauu uauaaguagu uga                                  3633


<210>  5201
<211>  3642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5201
auggagaaaa gguuagguau agauguuggg acuaacagcu uagguugggc cauaguggaa       60

guggacgaug aaggauguac acuuuuagau agaggagugg auauauuuca agaaggugua      120

gcucgagaga aaaaucagga aaaaccagca guucaagauc gaacagaugc uagagccuua      180

agaagacauu acuuuagacg aagauuacga aaaauagcac uccuaagagu gcuaguaaga      240

caugacuuau gcccagcccu aucagaagaa caacuaaacg cuuggcaagg uaaaggaauu      300

uauccauuag acgacgcauu uauaaggugg caacgaacag augacaaugc agaccguaau      360

ccauaucgag aucgcuuuag auguuuaaca gaagaauugg auuuggcaug uagagaagaa      420

agauacguac uagggagagc acuauaucac uuaugucaaa ggagaggcuu ucuuucaaac      480

agaaaggaug caggaaacga agcagaagaa ggaaagguua aacaagaaau uaaaacucuc      540

agugcagaua uggcagccgc agggugugaa uacuuaggag aauauuucua cagacuguau      600

caaagaaagg aaaagauucg aaaacauuau acagcaagga acgaacacua uuuagcugag      660

uucagagcga ucuguagaaa acaaagauua gaugaagaau uaaggcaggc auuagaaaag      720

gccauuuucu aucaacgacc cuuaaaaagu cagaaggggc uaguagguaa augcaccuuu      780

gaaaaagaca aaagucguug ccccgucuca cacccaagau uugaagaguu ccgaaugcua      840

caauucauua auaauguaag ggucacaggc ccaggugaug cagaaccaag accuuuauca      900

ccagaagaag uugaaauagu cagaccauug uuuuuaagga aaucaaaacc ucacuuugac      960

uuugaagaca uagcaaagaa aauagcugga aaaggaaauu acgcaugcag aggcgaacug     1020

cuucaagccc ccuauagauu caauugugca ggcaaaucga cuguaacagg aaguccaguu     1080

ucagcgcuau uugcaagcgu uuuuggagau gauuggcaaa gagaaauaug uucgcucuau     1140

acacugggug ccgguaaaaa ugaagaacaa auauuaaaug auguauggca cguauuauuu     1200

gcauuugaag acgaagaaag auuacaagca ugguuacagg aaaaacuaca auuaagugca     1260

gaugaugcaa aaacccuagc cgccuuuaga uuaccacaag gauacgccuc uuuguccuua     1320

aacguaauaa acaaaauauu accauacuug cgaagggguu auagauauga cgaagcugug     1380

uucuucgcua auuuaggaaa ggucuuacca gcggaaguau augcggaccc agcaaggcga     1440

agagaaguag agcaaggaau aagugcucaa auggacgacc uaggagaugg agaaaagaca     1500

aggaaagaag aacgcauaag agacuauuua agaagauacu ggggcgugga agagaaaaga     1560

cucgaaagac ucuaucaucc aucaaugauc gaggcuuauc caucugccuc accaaauggg     1620

caaggauugu uacaacucgg uucaccgagg auaccagcgg uaaggaaucc aauggcaaug     1680

cgcgcucuuu uuagauuaag gguuuugcua aaugaauuac ucagagaagg uaaaauugau     1740

agaggaacca aaauaaauau ugaauuggca agagaacuaa acgacgccaa uaaacgaaaa     1800

gcaaucgaac gauaccaacg cgagcgggaa gcugaaaacc gaaaauaugc ugaggaaaua     1860

cgacaacagu acguagaggc aacuggacac ucuauagaac caucagaaga agauauuuua     1920

aaauacaggu uaugggugga gcagggacac ugcucauuau auaccggcaa aacuauuggu     1980

auuagugaau uuuuaggaga ugguaggcga uaugacaucg agcauacagu accaagaagc     2040

cggggaggag aagacgcaca auauaacaaa acuuuaugcg aagaagaauu uaauagagac     2100

auuaaacgug auaaacuucc aagugaacua gcuaaucaca cugcaauacu cgcuugcguu     2160

gaaaguugcg gaugguucga gcgaauugaa ggacuugaac acaggguaug gggagcagac     2220

cgccgcgcaa agucagcgac uaccaaaagu gcaaaagacu uugcgaucca agacagacau     2280

uacuggagaa ugcacuugga cuauuggaga cgaaagugcg aagcauuuac aaugacagaa     2340

guaccagaag guuuuagcaa uagacaagga guagaugugg guauaauagg gcgauaugca     2400

agauuauauu ugcaaaccgu auuugacaga guauacacag uaaaaggggc cacuacugca     2460

gcauuuagaa ggaugugggg auuacaagag gaauacgcgg caaaagaaag ggugaaccau     2520

guccaccacu guaucgacgc gauuacaaua gcaugcauag gaucaagaga auacgcacuc     2580

ugggcacaau auguccgaca agacgaacuc uaucgauggg gagaagcaga uaaaccaaga     2640

ugcgggaaac cuuggccuac auucacggaa gauguaaggg caaccgcaga cgaauuauua     2700

auaucgcauc auacgccuga uaacaugccu aaacaaagca gaaaaagacu aagagugcgg     2760

ggcaaaguac aaagaaacga cagaggagaa guaaaauaua ugcaaggaga uacugcuagg     2820

ggagcauuac accaacaaac guuuuauggu gcaaucgaga gggacggaga gauaagauac     2880

guagugagaa aauccuuaga ucaauuacua ccugccgaug uugagaaaau uguagacgaa     2940

gcgguaagac aaaagguuag agaagcggua gaggcuuaug gauucaagga agcaacugac     3000

cccacaaaac acacaguuug gaugaaugag gaaaaacaaa uaccaauacg aaaaguucgu     3060

auuuuaacag gggugaaaaa uccuauacua uuaaaaccuc acagagacgu aucggucaaa     3120

gaauauaaaa gauauuacca ugucguaaac gacggaaacu auugcauggc cauauacgaa     3180

ggacgugaca gacaagggag acuaaaacga acguucgaga uaguaaauaa gcucgaagca     3240

gcccgguauu uuaaaagaag ugcugaucga gaaucaagac cugaucuagu accccucuca     3300

gaugucaacg gauauucauu aaaauaucuu uuaaagaccg gaacaauggu ucuauucuau     3360

gaaaacucuc cggaugaauu auaugagugu ucuguaggag agcucgcgaa aagauuauau     3420

aaaguaguag guauggcaca ggacggucga guacaauucu uguuccacca agaagcaaga     3480

gaccaaaaag cuguuacagc agaaugugga auggggauau ccguuuuuga ugcaagacau     3540

ccggcgccaa agcuaagaau cagaguguca aauuucaaaa uguuugucga agguuaugau     3600

uuugaauuaa caguaacagg agagguaaaa uuuaaacguu ga                        3642


<210>  5202
<211>  3642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5202
auggaaaaac gauuaggaau agacguagga acaaacucgu uaggaugggc aaucguagaa       60

guagaugacg aaggauguac guuacuugac agaggggugg auaucuuuca agaaggagua      120

gcacgagaga aaaaucaaga aaagccugcc guacaagacc gaaccgaugc aagggcuuua      180

cggagacauu acuuuagacg acguuuaaga aaaauagcac ucuuacgagu auuaguuaga      240

caugaucuau guccggcacu aagugaagaa caauuaaacg cuuggcaggg uaaaggaauc      300

uacccgcuag augacgcauu cauuagaugg caacgaacug augauaacgc ugaucgaaac      360

cccuaucgug auagauuuag auguuuaacu gaggaguuag acuuagcaug cagagaagag      420

agauacguau uagggcgagc auuauaccau uuaugccaga ggaggggauu uuuaucgaau      480

cgaaaagacg caggaaauga agcagaagaa gguaaaguaa aacaagaaau aaaaacacua      540

ucagcugaua uggcagcagc aggaugugaa uauuuaggag aauauuuuua uagauuauac      600

caaaggaaag aaaagauuag gaaacauuac acugcaagga acgaacacua uuuggcggaa      660

uuucgggcaa uauguaggaa acaaagauua gaugaagaau uaagacaggc acuagaaaaa      720

gcaauauuuu aucagagacc ucuaaagagu caaaaagguc uaguuggaaa auguacauuu      780

gaaaaggaca aaaguagaug uccaguguca cauccuagau uugaagaguu ccgaauguua      840

caauucauaa acaacguuag gguuacuggg ccuggcgaug cagaaccgag gccauuaucg      900

ccagaagagg uagagauugu acgaccguug uucuuaagga agaguaaacu acauuuugau      960

uuugaagaua uugcuaagaa aauagcagga aaaggaaacu augccuguag aggcgaacua     1020

cugcaagcac cguauagauu uaauugcgcc ggaaaaagca caguaacagg aagcccugua     1080

agugcauuau uugcgagugu auucggagau gauuggcagc gggaaauaug uucucucuac     1140

acacucggag ccggaaagaa ugaagaacag auccuaaaug auguauggca uguuuuauuu     1200

gcuuuugaag augaagaacg acuacaaaca ugguuacaag aaaaauugca auugucugca     1260

gacgacgcaa agacccuagc ugcauucaga uuaccgcaag guuaugcguc guuaucguua     1320

aauguuauaa acaaaauccu gccauaucua agacgaggcu auagauauga cgaggcagua     1380

uuuuucgcaa auuugggaaa aguucuacca gcagaaguau augcggaccc agcaagaagg     1440

cgcgaaguag aacagggaau aucugcgcaa auggaugauu uaggagaugg agaacaaaca     1500

agaaaggaag aaagaauaag agauuaucua cggagguauu ggggaguaga agaaaaacga     1560

uuagaaagac uguaccaucc gagcaugaua gaagcauauc cgagugcguc uccaaauggc     1620

caaggacucc uccaguuagg cucuccgcga auaccagccg uaaggaaccc aauggcgaug     1680

cgugcauuau uucgauugag gguacuacua aacgagcuac uaagagaagg caaaauugac     1740

agaggaacca aaauaaauau agaguuggca agggaauuaa acgaugcaaa uaaaagaaag     1800

gcaauagaaa gguaucaaag agaaagggag gcagagaaua gaaaauacgc agaagaaaua     1860

agacagcaau acgucgaagc aacaggucau aguauugaac caucggaaga ggacauauua     1920

aaauaucggu uauggguuga gcagggacac ugcagucuuu acacagguaa aacaauagga     1980

auaucugaau uuuuagggga uggacguaga uacgauauag agcacacagu accaagaagc     2040

agaggcggag aagaugcaca auauaauaaa acacuuugug aagaagaguu caaucgagau     2100

auaaagcggg auaaacuacc uagugaacua gcaaaucaua cagcaauuuu agcaugcgua     2160

gaaucaugug gaugguuuga acguauagaa ggauuagaac acagagugug gggagcugau     2220

cgaagggcua aaagugcuac cacaaaauca gcgaaagauu uugccauaca agauagacau     2280

uacuggagaa ugcaccugga cuauuggaga cguaaaugcg aagcguuuac aaugaccgaa     2340

guuccggaag gguuuucgaa uaggcagggu guugacguug gcauaauagg ccgauacgca     2400

agauuauauc uucaaacagu auuugaucgg guauauacag uuaaaggagc uaccacugcg     2460

gcauuccgua gaaugugggg uuuacaggaa gaauaugcgg cuaaggaaag ggucaaucac     2520

guucaucauu guauagaugc gauuacaauu gcauguauag gaucgcgaga auacgcguua     2580

ugggcucaau auguuaggca agacgaacuu uacagaagag gagaagcaga caaaccucga     2640

ugcggaaaac cauggcccac uuuuacagag gacguaagag caacugcuga ugaguugcuu     2700

auuucucacc auacaccaga uaauaugccg aaacagagua gaaagagguu aagaguacga     2760

gguaaaguuc agcgcaacga ccguggggaa gugaaauaua ugcaaggaga cacagcaaga     2820

ggugcccucc aucaacaaac cuucuaugga gcaauagaac gagaugggga aaucagauac     2880

guagugcgca aaucgcuaga ccaauuguua ccggcggaug uagagaaaau aguugacgaa     2940

gcaguacgac aaaaaguacg agaggcgguu gaagcauaug gauucaagga agcaacagau     3000

ccuacgaaac auacgguaug gaugaaugaa gaaaaacaaa uaccaaucag aaaaguucgg     3060

auacuuacgg gaguaaagaa cccaauauua uugaaaccac auagggacgu cucuguaaaa     3120

gaauauaaga gauauuacca uguuguuaac gauggaaauu auugcauggc gauauacgaa     3180

gguagagaua gacaaggaag acuaaaaagg acauucgaaa ucguaaacaa acuugaggcg     3240

gccagauacu uuaagagaag cgcggacaga gaauccagac cggauuuggu uccuuuaagu     3300

gauguuaacg gauauagucu aaaauaccuu uuaaaaacag gaaccauggu acuauuuuac     3360

gaaaauucac cagacgaacu uuacgaaugu ucagugggcg aauuagcaaa gaggcuauau     3420

aaaguaguug gaauggcaca agauggaaga guacaauuuu uauuucacca agaggcaaga     3480

gaucaaaaag ccguaaccgc agagugugga augggaauau caguauuuga ugcgcgacac     3540

ccagcaccaa aacuaagaau aaggguaucu aacuucaaaa uguucgucga aggauacgau     3600

uuugaacuaa cuguaacggg ggaaguaaaa uuuaagagau ga                        3642


<210>  5203
<211>  3651
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5203
augaccaaaa uucuaggaau ugauacuggc acaaacucac uaggaugggc aauugucgau       60

aaaacagccg auaacuacaa ucuacuagau aaagguguca acauuuucca ggaaggagua      120

aaaauagaaa aaggaaucga aucaaguaaa gcagcagaga gaacagaaca cagagcaacc      180

agagugagau auuacagaau aaaauugaga aaaauuagac uacuaaggau auuaucagau      240

aaccaccuau gccccccuuu gagucaagaa gaacuaucaa cauggcguuu aaaaaaagua      300

uacccaaaga augaacuuuu caugcagugg caaagaacag acgaugauac agaaaaaaca      360

ccauaugcau auagacauaa auguauacac gaaaagcuag acuuaagcga uguaaccgau      420

cguuacauau uaggaagggc auucuaucau auaauacaaa gaagaggguu ucucagcaac      480

agaaaagaac aagggggcga agauucagga aaagugaaag aaggcauauc aacuuuaaca      540

caugaaauga acgaggccgg cuacgaauac cucggugacu acuuuuacgc auuauacaau      600

aaaggcgaaa aaauuagaaa acauuauaca gcgcgaaaug aacauuucuu aucggaguuu      660

aaagcaauau gcgaaaaaca agauuuagag aaagaacucg gaucagaagu aguaagaaac      720

aucgaaaaag ccauauuuga ucaaaggcca uuaaaaucac aaaaaggaca aguaggaaaa      780

ugcguauuug aaaaaaacaa gacuaaaugc ccaucaucgc aucccaugua ugaggaauuc      840

agaauguuaa cauucauaaa caauaucaaa aucaagacac caaacgacga ugucuuacga      900

ccauuaaaca caggagaacg ugaaaaaaua augccauuau ucuuuagaaa aaguaaaaaa      960

caauuugacu ucgaggauau cacaaaaaaa cuugcaccaa aaaaaaugua uggauucuau     1020

aagaaagaag aagacgccga aaugccauuu cuauucaacu acccuaugga caccucggua     1080

ucgggguguc cagucacagc agcauuaaaa gacguguuug gagacgacug gauaacagcg     1140

auaugugaaa cauauacauu aggagagggu aaaagcagau ucgauauugu gaacgauauu     1200

uggcaugcac uauauuucua uagugacaag gacaaacuug cagaauuugc aauaaauagg     1260

cuccaauuaa gugaggauga agcuaaaauc uuuucagaaa uuucccuacc cgcagacuac     1320

gcuuccuuau cauuaaaagc aauuuguaag auacuaccau acuuaagaag aggcuuaaua     1380

uacucucaug cuguauucuu agguaaccug ugcgaaguaa ugccaaaaua cgaauggagc     1440

auaaaagaaa ugagagaaac aaucauagau aacauaauca uggaaaugga caaaugcgau     1500

acaaaagaca aaaggacauu ugaaguaugu auaaaagauu auuuaaaagg acaauauaau     1560

guuuccgaua auucgcuaaa aaaacuauac cauccuucaa ugauugaaac uuauccucgc     1620

guucaauaca cuaacgaaca cggaguuuac caacuaggaa guccaagaau agauaguguc     1680

agaaauccaa uggcaaugag aucaauguuu agacuaagaa aacuaguaaa uagacuuuua     1740

guugaaggua aaauagauca aaauacagaa guucauauug aauucgcccg agaacuaaau     1800

gaugcgaaua aaagaaacgc aaucgcucug uucguuaaag aaaaccaauc ugcaaacgau     1860

gaggcaagaa aacaaaucau aacuuuauuu aaaaaggaaa caggaaccga cauagaacca     1920

acggacaaag acauacuaaa auauguacuu ugggaagaac aaaaucacau auguuuauac     1980

acugguaaac aaauaagaau aagugauuuc auaggagcga auccaaaauu ugauauagaa     2040

cauacaauac caagaagcgu aggcggagac uccacuaaaa ugaaucuuac ucuaugcgau     2100

ucccguuuua auagagaagu aaaaaagaca aaacuaccag ucgagcuucc aaaucaugac     2160

gaaauaaugg aaagaauuaa agaauggaaa aaaaacuaug aauuacuaga uauucagauc     2220

cgcaaaaaua aaaaauuauc aaaaggcgca acaacaaaag aacaaaaaga uacaauuaua     2280

agaaaaagac auuuauugga acuaagaaga gacuacugga gaggcaaaua ugagagguuu     2340

acaauggaau cagucccuga aggauucagu agaagacaag gaacagauau aucagugaua     2400

aguaaauacg ccagauuaua ucuaaaaucc uuauuuaaca ggguuuauac cguuaaagga     2460

auugcaacgu cugauuuucg aaaaauaugg gguauucaag acauauacag caaaaaagaa     2520

agaauaaauc auguccauca cugcaucgac gccauaguca ucgcguguau cgguuuagau     2580

gaauacaaca aacuuggagc auacuaccac gacgaagaga acaauaaaug guacgggaug     2640

ucaaaagcuu cauuuaaaaa accuugggca acauucguag aagauaucaa aaaaauucaa     2700

gacgagauuu uagucuauca uuauacuccc gacaacaugc caaaacaagg acggaggaga     2760

auaaaaauag acggaaaaaa aauauuaugc aaaggugacg ccgcaagggg uucacugcau     2820

aaugacacau acuacggugc cauugaaaau gacggagcag uaaaauacgu caaaaggauu     2880

gaccuagcaa gcuuggagga gaaggacgua aagaauauug uugaugacac uguacgcucu     2940

auuauagaag ccgcuauaaa agaaaaaggc uucaaagaug caauggcaag cacaauaugg     3000

augaaugaag aaaaacgagu accaauaaaa aaagucagau guuuuacccc aagcauaacu     3060

aaaccauuaa acauacgaaa acaaagagau guaucaauaa aagaauacaa gcaacaauac     3120

cauguagcaa acgacucaaa uuaucuucuc gcacuauaca uuggcacaga caauaaagga     3180

aaggaaaaaa gagaguucga aaucguaaau auucuacaag cagcacaaua cuaccgaacg     3240

uccaacgaca aagaggucgu agacagacau auaguuccga uaaaaagcga acaugacuau     3300

ccuuuugcuu acacccuaaa aauuggcacc augguccuau uauacgaaaa aucaccuaau     3360

gaaguuuggg acgcaacgau caaggaacga aacagaagau uauauaaggu caccggccuc     3420

ucaacuauga gaaugaaagg gagaaacggu gaauacgcuu augcaacagu gaaauuaauu     3480

cauaaugaag aagcaagacc cucuaaagau aucaaagcaa aaaauggcga auacgagcaa     3540

ggagaagaau ucagaccggc aauaauuaug uuacacacuc aauuaaacgc acuaguucaa     3600

ggguaugacu uugaaauaaa ugagcuagga gaaaucagaa gacuaagaug a              3651


<210>  5204
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5204
auggaaaaac aaagaauauu aggucuggau ccaggaacaa acucacuagg gugggcuguc       60

uuggacuucg acaauagcaa acaggaauac aaucuaguaa gaaaaggcga aguaaucuuu      120

gaagaaggag ugggaaaauc cgagagggga gaauuuucaa aggcggcgua ccgaacauuc      180

uacaaaucaa caagaaaaca cuauuucaga cgaagacuca gaaaaauaga agucuugaaa      240

guacuaguaa aauaugauuu cugccccuau uuaucugacg acuuacuaaa acaauggcau      300

gaaaaaaaaa uauauccggc aguuaaagaa uucauggacu ggcaacgcac auccgacgcu      360

gaagguaaga acccauacua ugauaggcac uguugcuuaa acgaaaaauu agaucuaucu      420

cucaagagcg auagauacau auuaggacga gcucucuacc auuuaaccca acgaagaggc      480

uuuaaaucaa auagauuaau acaaaauaac gacgaaaaug aagaaggaaa aguaaaaaaa      540

ggcaucagcg aauuaucaac ugaaaugcaa gaagcaaacu guaacuaccu aggcgacuac      600

uucuacaaau uauauacaga aaaaggaaac accguacaga uccgcaaaag auacaccgac      660

agagaaaaac auuacaaaaa cgaguucaau gcaaucugug aaaaacaaga gcuaaaugaa      720

acgauaguca aagaauuaga uaaagccaua uuuuuucaac gaaaguuaaa accacaaaga      780

cacggaguag gaaaaugcac cuucgaaccc aacaacaggc guugugccau uagucauccc      840

gauuuugaaa gauuccgaau guuaugcuuc aucaauaaua uaaaaaucaa aacaccuuuc      900

gaagaaaauc ucagaccauu aaacaacgaa gagaaagcgu uaauagaacc acuguucuac      960

aggaaaagua uaaaaaacuu cgacuucgaa gaaauagcca aaaaacuugc gggaaaaaau     1020

aacuauugcu acaucaaaga cgacagcucc aaacaguaca aauuuaauua cagaaugucc     1080

caaggcgcaa gcaccugcca aacaauagcc aaacuaaaau caauauucgg agaagaaugg     1140

aaaaacggaa uagcagaaac auauacuaac agaagaaaaa aggaaggguu aaaaaaacaa     1200

gcagaaaugg uggaugauau cuggaacguc uuauauuccg ucucugaaug cgauguccuu     1260

aaagacuucg caauaaauaa auuacaauua gaugaaaaaa ccgcggauaa cuucucaaaa     1320

acaaaauugg cacaaggauu cgcaacauua ucuuuaaaag caauaagaaa aauacuaccu     1380

uucuuagaac aaggguucgg cuauacagaa gccguauucc uagcaaauau uccaacgguc     1440

gucggcaaag aauauuggaa uaacgaagac auaagaaaug acauaaugga ggaaauaaaa     1500

acaauauuuu accauaacac acucaaaaca aaugacucac acaauacaau ccucuucugc     1560

auuaaagaca uacuaagcaa uaacuuugac uuagcacccg gagccaucga caagcuauac     1620

cauccgucca ugauaaaugu auacaaagau gucauaccua acgaagaugg aauauuacag     1680

uuaggaucuc caaaaauaga ugcaguaaaa aacccaaugg caaugcguag ucuacacaua     1740

auucguaaag ucguaaacga acuauuacga gaggggauaa uaaacucaga cacagaagug     1800

cgaauagagu acgcaagaga gcuaaacgau gccaauaaaa gacacgcuau ugccgacugg     1860

cagaaaaaac aguaugggaa acaccaaaaa uaccguaagg agauaaaaga auuauucaaa     1920

gcagaaacug guuuugaaau agaaccaaac gaaacagaca uccuuaaauu cgaauuaugg     1980

gaagaacaaa accauauaug uuuguacacu ggucaagaga uaggaauaag cgaauucgua     2040

ggagaaaacc cgaaauauga uauugagcac acuuucccaa gaagccuagg aggagacuca     2100

acaaaaauga acaugacccu augcgaaaac aaguuuaaua gagaaaacaa acaaaaaaaa     2160

cuuccaagug aacuagcuaa ccacucugaa auucuagaac gaauugauga uuggaaaaaa     2220

aaaauauaua ucuuacaaaa aaaauaugac aguauacgaa cacacaacgg aauggacaaa     2280

gaaaauaaag acaaaaaaau aguuagccga cacaaaauua aauuagaauu agauuacugg     2340

aaaggaaaau auaaccgcuu cacgaugaca gaaauaccag aaggauucuc uagacgucaa     2400

ggcaugggaa ucggauuaau aucgagauac gcgggucuuu aucuuaaaac aauauuucau     2460

aaaaaggaca acccacaaaa aucaaaugua uacauaguaa aaggaagcau aaccgcugaa     2520

uuuagaagaa uauggaacau acaagacgac uacgagaaaa aaugcagaga uaaucacaua     2580

caucauugua uagaugcuau agucguugga ugcauaggaa aacgcgaaua cgacuuaaug     2640

gcucaguucu auagagauga agaaaaauau agaugggaaa aaaagaaaaa accuuucuuu     2700

aaaaaacccu gggaaacuuu cacucaagau augcucuccc uaaaagacga aguauugaca     2760

guacauuuca auccaucaaa uuuuacaaaa aaagcacaua aaaaaguguu cacgccaaag     2820

ggaauauucg uagcccaagg cgauugcgca agaguaaaac uucacaaggg uaguuacuuu     2880

ggagccauug agcaaaaagg cgaaauuaaa uacguaaugc gaaaagaacu aagugcauua     2940

aaaauagaag auauaaaaaa uauaguagac gccgucguaa aagaaaaagu ucuagcugua     3000

guaaaaaaaa aagguuucaa gcaagccaug gcagaaccaa uauacaugaa ugaagacaaa     3060

agaauacuaa uuaaaaaagu acgacuguuc guaagucaag cuaauccacu aauagugaaa     3120

aaacacagag acuuaucauc gaaagaauau aaacaaaacu acuacguuga caaugaaggu     3180

aaucucauga uugccaugua cgaaggugua aaaaaaaacg gaaaaauaga cagggaaauc     3240

acaguuguca acaaucuaga ggcugccaaa uuuuuccguc agagucaaaa aaacaacgcg     3300

gaaaaacagc uaauaucuca ccuaucuccu aaaaacgguu accccuuaaa aaccguccua     3360

acccaaaaac aacuaguccu uauguacgaa gacagcccua aagaaauaaa acuccgggac     3420

acaaaaaaca ugguaaaaag auuauaucaa guauuuggaa uagaaaaaga cggcagggua     3480

aaguuaaaau uucaccaaga agccagauca gaagguuuaa acauaaacag cgccgcauuc     3540

aaaauucaag auacaccuga aucacuauau cgacauacaa aaagcaaccu aaaaguacua     3600

gucaacggag uagauuucaa aaucaacaua uuaggcgaaa uaagccuaau uuga           3654


<210>  5205
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5205
auggaaaaac aaagaauucu cggucuagau ccaggaacca acucauuagg cugggcagua       60

cuggacuuug acaacucaaa acaagaauac aaccuaguga gaaaaggaga aguaauuuuc      120

gaggaaggag ucggaaaauc agaaagagga gaauucagua aagcagcuua uagaacauuu      180

uauaaaucaa caaggaaaca cuacuucaga agaagacuca gaaaaauaga agugcuaaaa      240

guauuaguaa aauaugacuu uugucccuau cugucagacg accuguuaaa acaauggcac      300

gaaaaaaaaa uuuauccugc aguaaaagaa uucauggauu ggcaaagaac aucagacgcu      360

gaagggaaaa aucccuauua cgacagacau ugcugcuuaa acgaaaaauu agauuuaagu      420

cucaaaagcg aucgauacgu auuggguagg gcccuauauc auuuaacaca aagaaggggu      480

uuuaaaagua accgcuuaau acaaaacaac gaugaaaaug aagaaggcaa agugaaaaaa      540

ggaaucuccg aacucaguac agaaaugcaa gaggcuaauu gcaacuauuu aggugacuau      600

uuuuauaagc uauacacaga gaaaggaaac acuguacaaa uacgcaaaag auauacugac      660

agagaaaagc acuauaaaaa ugaauucaau gccauaugcg aaaaacaaga acuaaacgaa      720

acaaucguca aagaacuuga caaagccaua uucuuucaaa gaaaauuaaa gccacaacgu      780

cauggaguag ggaagugcac auucgaacca aacaaucgac guugcgcaau uagucacccg      840

gacuucgaac gcuuucgaau gcuuugcuuc auaaauaaua uaaaaauaaa aacuccauuu      900

gaagaaaacc uuagaccguu aaacaacgag gaaaaagcac ugaucgaacc uuuauuuuau      960

agaaaaucaa ucaaaaacuu cgacuuugaa gaaauagcaa agaaacucgc uggaaaaaac     1020

aauuauugcu acauaaaaga cgacaguuca aaacaauaca aauuuaauua uagaaugagc     1080

caaggagcau cgacgugcca aacaauagcc aaacuuaaaa gcaucuuugg ugaagaaugg     1140

aaaaacggua uagcagaaac guauacuaau cgaagaaaga aagaagguuu aaaaaaccag     1200

gcagaaaugg uagacgacau auggaacguu cucuacucgg uaucugaaug cgaugugcua     1260

aaagacuucg caauaaauaa acuacaauua gacgaaaaga cagcagauaa uuuuagcaaa     1320

acaaaauuag cacagggauu cgccacacua agcuuaaaag caauacgaaa aauacuccca     1380

uuccuagaac aagguuuugg uuauacagaa gcaguuuuuu uagcuaacau accaacagua     1440

gucgggaaag aauauuggaa caacgaagac auaagaaaug acauaaugga agaaauaaaa     1500

acgaucuuuu accauaacac acuaaaaacg aacgacagcc acaacacaau acuauucugu     1560

aucaaagaca uucuaaguaa caacuucgac cuagccccug gugccauaga caaacuauau     1620

cacccaucua ugauuaaugu auauaaagac gugauaccca augaagacgg uauccuacaa     1680

cucggcagcc caaaaauuga ugccguuaaa aaccccaugg cgaugagauc acuacacauu     1740

auacggaaag uugugaauga acugcugcgu gaaggcauaa uaaauuccga cacagaagua     1800

agaauagaau acgcaagaga auuaaacgac gccaacaaga gacaugcuau ugcugacugg     1860

caaaaaaaac aguauggaaa acaccaaaaa uacagaaaag agauaaaaga acuauucaaa     1920

gcagaaacug gauuugaaau agaaccgaau gaaacagaua uacuuaaauu cgaacucugg     1980

gaagaacaaa aucauauaug ucuauauaca ggacaagaaa uaggcauuuc ggaauucguu     2040

ggagaaaauc caaaauauga cauugaacac acuauaccaa gaaguuuagg aggcgauuca     2100

acuaaaauga auaugacguu gugcgaaaac aaauuuaacc gagagaauaa acaaaaaaaa     2160

uuacccucug aacuugcaaa ucacucagag auacucgaaa gaauagacga uuggaaaaaa     2220

aaaauauaua ucuuacaaaa aaaauaugac ucuaucagaa cacacaaugg aauggacaaa     2280

gaaaacaaag acaaaaaaau cguuaguagg cauaaaauua aacuugaauu agauuauugg     2340

aaaggaaagu auaauagguu uaccaugacc gaaauaccag aaggauuuuc aagacgacaa     2400

ggaaugggua uuggucuaau cagccgauac gcaggacuau accuaaaaac uaucuuucac     2460

aaaaaagaca auccacaaaa gagcaacgua uacauaguua aaggaucaau cacagcugaa     2520

uucagacgaa uuuggaacau ucaagaugau uacgaaaaaa aaugcagaga uaaucauaua     2580

caccacugca uagacgcuau agucguuggu ugcauaggua aaagagaaua ugaucuaaug     2640

gcacaauuuu acagagacga agaaaaauau cgcugggaaa aaaaaaaaaa gccauuuuuu     2700

aaaaaacccu gggagacauu cacacaagac auguuaagcc uaaaggacga gguccucaca     2760

guccauuuca auccuagcaa cuucacuaag aaagcacaca aaaaaguauu cacaccuaaa     2820

gggauauuug uagcccaagg cgacugugca agagucaaac uacauaaagg aaguuacuuu     2880

ggggcaauug aacaaaaagg cgaaauaaag uauguuauga gaaaaaaacu aucagcacua     2940

aaaauagaag auaucgaaca uauuguagac gcaguaguga aagaaaaagu acuagcagug     3000

guaaaaaaga aaggauuuaa gcaagccaug gccgaaccaa uauacaugaa ugaagacaaa     3060

agaauacuua uaaaaaaagu acgauuguuu gucucucaaa cgaacccucu aauaguaaaa     3120

aaacacagag aucuuucauc aaaagaauau aaacaaaacu auuauguaga uaaugaagga     3180

aaccucauga uugcaaugua cgaaggaguc aaaaaaaacg gaaaaaucga cagagaaaua     3240

acaguaguua auaaucuaga agcagccaaa uucuucaggc aaucgcagaa aaauaacgca     3300

gaaaaacaac uaauuucaca ccugagucca aaaaauggau acccacuaaa aacuguccuc     3360

acacaaaaac aacuaguacu aauguacgaa gauagcccca aagagauaaa acuacgagac     3420

acaaagaaua ugguaaaacg ccuauaccaa guauucggaa uugaaaaaga uggacgagua     3480

gaguuaaaau uucaccaaga agccagauca gaaggucuga acaagaacag ugcggcguuc     3540

aaaauucaag acacacccga aucccuauau cgacauacaa aaucaaauuu gaaaguucuu     3600

guuaacggcg uggacuucaa aauuaauauu uuaggugaaa uaucauuaau auga           3654


<210>  5206
<211>  3654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5206
augaaauaua acuauguacu agggcuggau auaggaauua guucaguugg augggguuua       60

cuagcacuag augaaaacaa caacccuuac aaaauaaucg acguaggauc cagaauauuu      120

uccccaggag aaguggagaa aacaggagac ucaaaagcaa aagaaagaag agagaaaaga      180

ggaacaagaa gaguaauacg cagaagagag uucagacucg auagaguaag aaaucuacuc      240

uacgaaaaag gauaucuaaa agguaacaua acaaguaaca ucguuucagu caaaaacgaa      300

gaauuaacaa uuaucuucaa cgacauggug aauaauuacu acaaauccaa agauacaaau      360

ccguauaaac uuaaaguaga agcgcuggau aggaaacuaa caaaugauga auuaucuaua      420

auauuaguac auuaugccaa gaaaagaggg uacaaaagca auagagaaga aaguagugac      480

aaugacaacg guaaaguaaa agaagcaaua aaagaaaaca caaaucuaau ggaagaaaag      540

aaauacagga caauaagcga aauguacauu aaaaacgaaa aauuuaaaaa uaaaauuaaa      600

aauggaccag augacuacaa gcuaucuguu acaaacgaaa uguaucuaga agaaauaaac      660

aaaguauuag acagccaaau aggauuugga cuaauugacg acaacuuuaa aaacgaauau      720

uuagaaaucu acaacagccg cagacacuac uccgaagggc ccggauauua uuacgcgaaa      780

gacgcaaacg gaaauaaagu uaaagucaga agcccauacg guggugaccu aauuaagaaa      840

augacgggag uaugcuccuu cgauaagaaa gccagagcgc ccaaauaugc guucagcuca      900

gaauuauucg uaucaaucuc aaaguuagua aaucugcguu acaaaauaga ugacgacagc      960

uauaaacaac ucaccaaaga acaaauuaac gaacuuaucg acgaagcuaa aaauaaaaau     1020

aaaguuacuu acaaaucacu aauaaaaaua cuaggaauca aaaacauuaa aguaaaagac     1080

cuaaaucuca guaaaucuga auauauaaaa guuauagacg aacuaaagaa gaaacugaac     1140

auaucaaaag aagagaaaau ugacauauac ucacucgacg aagaaaacaa gaaaauauau     1200

gacgauauau auaaugaaaa acuuuucuca aaaacacuua uagaauuuaa aggauacaau     1260

cuacuaaaga aagcaauaac acuaagcuau aacagcggca cuuggaacga cguaaaagac     1320

aauauagagu uccuagauga acuggcacua uacuguacag auuauaaagu aaacaaugaa     1380

auucaggaac auauacauaa cucacuaauc aucgacucca gauucgauga uucaaauuuc     1440

gucgaaaacc uaccaaauuu caaagaccac cuuaugcuau caacagauau cauuagaaaa     1500

cuaaucccca uaauguuaga aggaauaaca uacgauaaag cgauggacaa aauaggguac     1560

aaccauagca accucuacaa agauacaaua aaagaagauc uguuagugcc aguauauguu     1620

uccaaaaaua ucaccaauca aagagucauc cgaucauuaa cccaagcacg aaaaguaaua     1680

aaugcaauaa ucaagaaaua cgguagcccu aaaaucauua acaucgaaac cgcaagagaa     1740

uuagccaaaa cacaaaaaga aaggcguauu aucgagaaaa aucaacaaga caaacaauac     1800

gaaaacgaac gaauaaagaa acacuuagug gaacuagaau uguucucaga cgaaagcaaa     1860

auaacaggaa acgacuugcu aaaauacaaa cuauggcaag aacaaaaaga guucugcggc     1920

uacaguauga agaaaaucaa acuggaagac cuauucagca auaacauagu acaaauagac     1980

cauauauuac cauacucacg aaccuauaau gacaauuacu uaaacaaaac acugguauau     2040

acuaaauaca accaagacaa aggaaacaaa acaccauacg aaugguucgg aaaaacagac     2100

aaauggcaag aauacgaagc guucauaaau agccugucga uaagccaaaa caagaaagau     2160

aacuaucucc uuaaaaaccu agaucaagac gcugaacgag aaaugagaga gcaaaaccua     2220

aacgauacca aauauauauc aagggagcua uuaucguuaa uaaaagcaua ucuuaacguu     2280

gacgaaguaa augcauuccc cggagcaauc accgcaaaau uaagagcacg auggggcuuc     2340

aacagauuaa cccauuccua uauaagcaaa accuauauca ugcccgaaga caugaagaaa     2400

ggaauaaaca aagauagaga caaccacuua caccacgcca uggaugcgcu cguuauagca     2460

agcaucacaa aaucacugca gcagaaaaua acccuuuacg aaaaauuuuc cagauacgua     2520

gauggaauaa ccaaaaacga acuagggaaa auauccacgg gaaacauaaa ugaugaucuu     2580

uacaacuauu acgaugaaga aacuggagaa aucaaagaac uagaacucag acaauacauu     2640

aaaaaccaag uugcccaaaa caauauuuac uuuucuaaac acaacguauc caaacuaaua     2700

uucccaacac cauauaacaa cuuugucgaa gaggcaaaac uuaggguaua ugaaagagau     2760

cucgaaguac uuaaauggaa ucugaaaaau cuaggaacau acaccagcag cgaacuaaac     2820

aggauucacg uauuaauacc aaguguugcu aaaaauaaaa uaaguggacg auuacacgaa     2880

gaaacauacu auggacucaa agaagugggu aucggagagg auaaaguaac uuacaaaacc     2940

cucaaaacac cuaucaguaa agucaaaaag aaagaucucg auaauauacc cgacugcaaa     3000

gguggaucaa aagacauaua uaaugcuuua guaaaauggu uuggaaacug cgagacugga     3060

gaagaagcac uaaaaaugca cgaugguaaa uauccuguca acccaaacga uaaagaacaa     3120

aaagaaauca gaaaaauuaa aguauacaca aaauacaacg cgaccgggca cguaguaaac     3180

aaugguaacg uugaaaaagg aggaauauau agaaucgaug uauucaaaag caaaaauaaa     3240

gaagacaaua aacuuuacuu cacaucauau gauauauuug aaauagaaca aaucaaggcu     3300

uacgaaaaag gcaaaaauau caacuuuagc auaaaacuag aauauggcca agguaaaaau     3360

aauaaaauac uacuauacaa agacgugaaa aacgaauacg acuuaauaau gaguauauac     3420

aaaaacgacc uaauaagaau aaaaacaaaa acaggaaaua aauccacagc cuauguagua     3480

ggcuucucag gagguaaacu ggagauaaaa aguacaauug gagaugggua cgauauaaua     3540

ggcaauaaua acauauucga caaaagcaug gagagguaua gaguaaccag uagcacgaua     3600

guaaaaaucg acaaauuaag cauaagcaua cugggugaag uaagcgguuu auga           3654


<210>  5207
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5207
augaacaaaa gaauacuagg auuagauaca gggacaaaua gccuaggcug ggcaguggua       60

gauugggaug agcacgcgca auccuacgaa uuaaucaaau acggagaugu caucuuucag      120

gaggguguua aaauagaaaa agguaucgaa ucuucaaaag ccgcagaaag aucuggauau      180

aaagcuauua ggaaacaaua cuuuagaagg cgacugagaa aaauacaagu auuaaaagug      240

cuuguaaaau accaccugug uccauaccua ucagacgacg aucuuagaca auggcauuua      300

cagaaacagu acccaaaauc ggaugaacua auguuauggc agagaaccuc agaugaagaa      360

gggaaaaauc cauauuauga ccggcacaga uguuuacacg aaaaacugga ccucacagua      420

gaggcagaca gauacaccuu agguagagcc cuauaucauu uaacacaaag aagaggauuc      480

cuuucuaaca gauuagacac aucggcugac aacaaagaag auggaguugu caagucagga      540

auaagccaau uaucuacgga aauggaagaa gcaggaugug aauaucuagg agauuauuuu      600

uauaaacuau accaugagca agguaauaaa guaagaauac gacaaagaua uacagacaga      660

aagaaacacu accaacacga guuugaugcu aucugcgaaa agcaagaauu aucuagcgaa      720

uuaauagaag acuuacaaag agcaaucuuc uuccaacugc ccuuaaaauc ucaacgacau      780

ggaguuggaa gauguacauu cgaacgggga aaaccacgcu gugcugauag ccacccagac      840

uacgaagaau uuagaaugcu auguuuugug aauaauauac aaguaaaagg accacaugac      900

cucgaacucc gcccacuuac auacgaagaa agagaaaaga ucgaacaucu cuucuuuaga      960

aaaaguaaac caaauuuuga cuuugaagac auagcaaaag aauuagccgg uaagaaaaac     1020

uaugccugga uacacgauaa agaggagcgc gcauauaaau uuaacuaccg aaugacccaa     1080

ggggucccag ggugcccaac caccgcacaa cuaaaaagua uuuucggaga cgauuggaaa     1140

acuggaauag cagaaacaua uacacuaauu caaaagaaaa acggaaguaa aucacuccaa     1200

gaaaugguug augacguaug gaauguacuc uauucauuca guucgguuga aaaacugaaa     1260

gaauuugcac accauaaacu acaacuugac gaagaaaaug cugagaaauu ugcgaaaaua     1320

aaauuaucac acagcuucgc agcauuaagu uuaaaagcua uaagaaaauu ccuacccuuc     1380

cuaaggaaag ggauguacua uacacacgcc agcuucuuug ccaauauccc cacuaucguc     1440

gguaaagaaa ucuggaacaa ugaacaaucu agaaaauaua uuauggaaaa uguuggggag     1500

cuaguguuca acuaccaacc caaacauaga gacguccagg gaaccaucga aauguuaaua     1560

aaagauuucc uagcaaauaa cuucgaacua ccagcaggag caacagacaa gcuauaccac     1620

ccaucaauga ucgaaaccua uccucacgcg cuaaggaaug aauuugggau acuccaacua     1680

ggcucaccua gaacaaaugc cauucguaac ccaauggcaa ugagaucauu gcaugugcuu     1740

agaagaguug uuaaccaacu auuaaaagaa uccauaauug acgaaaauac ugaaguacac     1800

guagaauaug cgagagaauu aaaugacgca aauaaacgua gagccauagc cgauagacaa     1860

aaagaacaag acaaacaaca caagaaauac ggggacgaaa uacgaaaauu guauaaagaa     1920

gaaaccggaa aagauaucga accgacacaa acugauguac uaaaauucca acuaugggaa     1980

gaacagaauc acuauugcuu auauaccggc gaacaaaucg gcaucacuga uuuuauagga     2040

ucaaacccaa aauucgauau agaacauaca auaccacaau caguuggagg agacaguacc     2100

caaaugaacu uaacuuuaug cgauaauaga uuuaauagag aagucaagaa agcaaaauua     2160

ccaacugaac ucgcaaauca cgaagagaua uugacgagga uagaaccaug gaaaaauaaa     2220

uacgaacaau uaguaaaaga aagggacaaa caaagaacgu ucgcuggaau ggauaaagcu     2280

guaaaagaca uaagaauaca aaaacgacac aaauuacaaa uggaaauaga cuauuggcgc     2340

ggaaaauacg aaagauucac aaugacagaa gucccagaag gauuuucaag aagacaagga     2400

acuggcauug gauugauauc uagauaugcc ggucucuauc ucaaaucacu auuucaccag     2460

gcagauuccc gaaauaaaag uaacgucuau guagucaagg ggguugcuac cgcagaauuu     2520

agaaaaaugu ggggccuaca aucugaauac gaaaagaaau gcagagacaa ucacucacac     2580

cacuguaugg augcuauaac cauagcaugu auuggaaaaa gagaguacga ccuaauggcc     2640

gaauacuauc acauggagga aacauuccga caaggaagag guagcaaacc aaaauucuca     2700

aagccauggg caacuuucac agaagaugua cuaaauaucu acaaaaaucu auuagucguc     2760

cacgacacac cuaauaauau gccuaaacac acuaagaaau auguccaaac uucaaaagga     2820

aaaguauugg cacaaggaga cacagcacga ggaucacuac auuuggauac cuacuacgga     2880

gcaauugaac gagauggaga aauccgauau guaguaagac gcccacugag uagcuuuaca     2940

aacccaaaag aauuagaaaa cauaguagac gaaaccguaa aaagaaccau caaagaggca     3000

aucgcagaca aaaauuuuaa gcaagcaaua gccgaaccua ucuacaugaa ugaagacaaa     3060

ggcauucuca uuaagaaagu aagaugcuuu gcaaauagcg ugaaacagcc aauaaacaua     3120

aggcaacaca gagacuuaag caagaaagaa uacaaacaac aauaucaugu aaugaaugaa     3180

aauaacuauu uauuagcgau auacgaagga uuaguuaaaa acaaaguagu aagagaguuu     3240

gaaauuguau cuuacaucga ggcagcaaaa uauuauaaaa gaagucaaga cagaaauauu     3300

uuuaguucaa uuguaccuac gcauaguaca aaauacggau uaccuuuaaa aacaaaauua     3360

cugaugggac aacaaguauu aauguuugaa gaaaauccag acgaaauaca gguagauaac     3420

acaaaagauc uaguaaaacg uuuauacaaa guggugggaa ucgaaaaaga uggacguaua     3480

aaauucaaac accaccaaga ggcuagaaag gaaggauuga cuauuuacag uaccccauau     3540

aaaaacaaug acgauuaugu cccaauuuuu cgucagucaa uuaaaaacau uaauauauua     3600

gucgauggaa uagauuuuaa uauugauauc cuaggaaaag uaacccuaaa agaauga        3657


<210>  5208
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5208
augaacaaaa gaauauuagg cuuagacacc ggaacaaauu cucuaggaug ggcaguaguc       60

gacugggaug aacaugcaca aucauaugaa cuaaucaaau auggagacgu cauauuccaa      120

gaaggaguaa aaauagaaaa aggaauagaa agcagcaaag cagcagaacg auccgguuac      180

aaagcgauaa gaaaacaaua cuuuagacgg agauuacgaa aaauccaagu acugaaagua      240

uuaguaaaau aucauuuaug uccauaucuc ucagacgacg acuuaagaca auggcaccuc      300

caaaaacaau auccaaaaag ugaugaacua augcucuggc aacguacauc agacgaagaa      360

ggcaaaaauc cauacuacga cagacauaga ugcuuacacg aaaaacuaga cuuaacggua      420

gaagcagaca gauauacacu cggcagagcc cucuaucacc ucacccagag gagaggauuc      480

cuaucaaacc gguuagacac aucugcagac aauaaggagg acggugucgu aaaaucaggc      540

auaucacaau uaucgacgga gauggaagag gcaggcugcg aauaucucgg cgacuacuuu      600

uauaaauugu augacgcaca ggggaauaaa gucaggauaa gacaaagaua uacggacaga      660

aauaaacauu accaacacga guuugaugca auaugcgaaa aacaggaacu aagcagugaa      720

cuaauagaag acuuacaaag agcaauuuuc uuucaauuac cucuuaaauc gcaaagacau      780

ggaguagguc guuguacuuu cgaaagagga aagcccagau gcgccgauag ccacccagac      840

uaugaagaau uucgaaugcu guguuucgug aauaauauac aaguaaaagg uccccacgac      900

cuagaacucc gaccacuaac guaugaagag agagagaaaa uagaacccuu guucuuuaga      960

aaaucuaagc caaauuucga uuuugaagau auagcaaaag cacucgcagg caaaaagaac     1020

uaugcaugga uucacgacaa agaagaaagg gccuacaaau uuaauuaucg aaugacacaa     1080

ggaguaccag gaugucccac uauagcacaa cuaaaaucaa uauuuggcga cgacuggaaa     1140

acaggaauug cagaaacaua uaccuuaauc caaaagaaaa auggcagcaa aucuuuacag     1200

gaaaugguag acgacguaug gaaugucuua uacucauuuu caucaguaga aaaacucaaa     1260

gaguucgcac accacaaauu gcaauuagac gaagaaucag cggaaaaauu ugccaaaaua     1320

aaauuaucuc auucauucgc agcuuugagc cuaaaagcaa uacguaaauu ccuaccauuc     1380

cuacguaaag gcauguacua cacccaugca ucauucuuug caaacauacc uacaauagua     1440

ggcaaggaaa ucuggaauaa agaacaaaau cgaaaauaca ucauggaaaa cgugggugag     1500

cuaguauuua acuaucaacc caaacacaga gaaguacaag gaaccauaga aaugcuaaua     1560

aaagacuucc uagccaauaa cuuugaacua ccugcuggag caacugacaa acucuaccau     1620

cccagcauga uugaaacuua uccaaaugca caacgaaacg aauuuggaau ccuacaauua     1680

gguucgccga gaacaaacgc aaucagaaau ccgauggcaa ugcgaagucu acacaucuua     1740

agaagaguag uuaaucaauu auuaaaagaa ucaauaauag augaaaauac agaaguucac     1800

guagaauacg cucgggaguu aaacgaugca aacaaaagga gagcaauagc agacagacaa     1860

aaggaacaag auaaacaaca caagaaauau ggugaugaaa uaagaaaacu uuacaaagag     1920

gaaacaggaa aagacauaga accuacccag acugacgucc uuaaguuuca acucugggaa     1980

gaacaaaacc aucacugcuu auauacgggc gaacaaaucg gcauaacaga cuucauagga     2040

ucaaacccua aauuugauau agaacauaca aucccgcaau caguaggagg ugacucaaca     2100

caaaugaacu uaacucuaug cgauaacaga uuuaaccgag aaguaaagaa agcgaaauua     2160

ccaacagaac uugcgaauca ugaagaaauu cuaacuagaa uagaaccaug gaaaaacaaa     2220

uaugaacagc uagugaaaga gagagauaaa caaagaacuu uugcaggaau ggauaaagca     2280

guaaaagaca ucagaauaca gaaacgacau aaauugcaaa uggaaauaga uuacuggaga     2340

ggaaaauaug aacgcuucac aaugacggaa guaccagaag gauuuaguag aagacagggc     2400

acuggcauag gauuaauauc ucgauacgca gguuuauacc ucaaaucauu auuucaccaa     2460

gcagauaguc guaauaaauc aaacguauau guaguaaaag guguagcaac agccgaauuu     2520

cggaaaaugu ggggauuaca aucagaauac gaaaagaaau guagagacaa ccacagccau     2580

cauugcaugg acgcuauaac cauagcuugu auaggaaaaa gagaauacga ccuaauggcu     2640

gaauacuacc gaauggaaga aacauucaaa cagggcagag gaagcaaacc caaauuuagc     2700

aaaccguggg ccacuuuuac ggaagacgua cucaacaucu acaaaaauuu acucguugua     2760

caugauacuc caaauaauau gccuaagcac acaaagaaau acguucaaac cucaauagga     2820

aagguacuag cacaaggcga caccgcccga ggaucgcuac acuuagauac auacuaugga     2880

gcaaucgaac gagacggaga aauuagauau guggugagga gaccucuuuc aucuuucacu     2940

aaaccagagg aacuagaaaa cauugucgac gaaacuguga aaaggacaau aaaagaagcc     3000

auagcagaca aaaacuucaa gcaagcaaua gcagaaccca uauauaugaa cgaagaaaaa     3060

ggaauacuua uuaagaaagu ucgcugcuuc gcaaaaagug ugaaacaacc aaucaacaua     3120

agacagcaca gagaucuaag caagaaagaa uacaaacaac aauaccacgu aaugaacgag     3180

aauaauuauc uucucgcaau uuacgaaggc cuaguuaaaa acaaaguagu aagagaguuc     3240

gaaauaguau cauacaucga agcugcaaaa uacuauaaaa gaucccaaga uaggaacaua     3300

uucaguucaa uuguucccac ucauagcaca aaauaugggu uaccacuaaa aacaaaauua     3360

cucaugggac aauuaguacu gauguucgaa gaaaauccgg acgaaauuca gguagauaac     3420

acaaaagacc ucguaaaaag auuauauaaa guaguuggaa uagaaaagga cgggagaauc     3480

aaauucaaau aucaucaaga agcuagaaaa gaaggacucc ccauuuuuag uacacccuac     3540

aagaacaacg augauuaugc cccaauauuc agacaaagua uaaacaauau uaauauucua     3600

guagauggaa uagacuuuac aauagacaua cuugguaaag ucacguuaaa agaauga        3657


<210>  5209
<211>  3657
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5209
augaauaaaa gaauacuagg acuagauaca ggaaccaacu cacuaggcug ggcaguaguc       60

gauugggaug aacaugccca auccuaugag uucauaaaau acggagaugu cauauuccaa      120

gaaggaguaa aaauagaaaa agggauagaa uccucaaaag cagccgaaag aucaggcuac      180

aaagccauac gaaaacagua uuuuagaaga cgacuaagaa aaauacaagu acuaaaagua      240

uuagucaaau aucauuuaug cccauaucuu agugaugacg auuuaaggca auggcaccua      300

caaaaacaau acccaaaauc agaugaacua augcuuuggc aaagaacaag cgacaagaaa      360

gguaaaaacc cauacuacga uaggcauaga ugucuucacg aaaaacuuga ucuaacagua      420

gaagccgaca gauauacccu aggucgugca uuauaccacc uaacacagcg ccgaggcuuu      480

cuaucaaaca gauuagauac cucugccgau aacaaagaag acggaguagu aaaaucagga      540

auaagucaau uaaguacuga aauggaagag gcuggaugcg aauaucuagg agacuacuuc      600

uauaaacucu augacgagca agguaauaaa guacgaauaa gacaaagaua uacagaucga      660

aacaaacacu accaacauga auuugacgcc auaugugaga agcaagaacu caguuccgaa      720

uugauagaag acuugcaaag agcaaucuuc uuucaacucc cacucaaauc ccaaagacac      780

gguguuggga gaugcacauu ugaacgagga aaaccgagau gcgcugauuc ucacccagau      840

uacgaagaau uuaggauguu guguuuugua aauaauauac aagugaaagg cccacaugau      900

cuagaacuua ggccauugac uuaugaggaa agggagaaaa uagaaccccu guucuuuaga      960

aaaagcaagc cgaacuuuga uuucgaagac auagcaaagg cacuagccgg gaagaaaaac     1020

uaugcaugga uucaugauaa agaagaaaga gccuauaaau ucaauuauag aaugacgcaa     1080

ggaguaccug gauguccuac aacagcacaa cugaaaucca uuuuuggaga ugacuggaaa     1140

accggaauag cagaaacaua uacguuaaua cagaagaaaa acggaucaaa aaguuuacaa     1200

gaaauggaag augauguaug gaauguuuua uauagcuucu caaguguaga aaaauuaaaa     1260

gaauuugccc aucacaaauu acaauuagau gaagaaucag ccgaaaaauu ugcgaaaauu     1320

aaacucaguc auaguuuugc agcccuauca cugaaagcaa uaagaaaauu cuugcccuuu     1380

cuaaggaaag gaauguacua uacucacgcg agcuucuucg caaacauucc aacaauagua     1440

ggaaaagaaa uauggaacaa cgaacaaaac cgcaaauaca uaauggaaaa uguuggagag     1500

cuagucuuca auuaccaacc aaaacauagg gaaguucaag gaacaauaga aauguuaauc     1560

aaagauuuuc uggcaaacaa cuucgaacug ccagcaggag caacagauaa auuauaccac     1620

ccaucaauga uagaaaccua uccacaugca caacguaacg aguucgggau acuacaacug     1680

gguucaccaa gaacaaacgc cauaagaaau ccaauggcca ugagaucacu gcacauuuua     1740

cgaagagucg ucaaucaauu acugaaagaa ucuauaauag acgaaaauac agaaguacac     1800

guagaauacg caagagaacu uaaugaugca aacaaaagac gagcgauugc aggcuggcag     1860

aaagaacaag acaaacaaca uaagaaauau guggacgaaa uacguaaauu auauaaagaa     1920

gaaacgggca aagacauuga accaacccaa acagauguuu uaaaauuuca auuaugggaa     1980

gaacaagacc aucauugcuu auauacagga gaacaaauag gcauuaccga cuucauaggc     2040

ucaaacccua aauucgauau agaacauaca auaccacaaa gcguaggugg agacaguacg     2100

caaaugaauu uaacauuaug cgacaacaga uuuaacagag aaauaaagaa agccaaacug     2160

cccgcgcagu uaagcaacca cgaagaaaua cuagcccgaa ucgaaccuug gaaaaauaaa     2220

uaugaacaga aaauaaaaga aagggacaaa caaagaaccu ucgcuggaau ggacaaagcg     2280

gucaaagauc uacgcauaca aaaacgucac aaacugcaaa uggacauuga cuacuggaga     2340

gguaaauaug aaagauuuac caugacugaa guacccgaag gauucucaag aagacaaggg     2400

acagggauag gauuaauauc gagauacgca gguuuauacu uaaaaagccu auuccaucaa     2460

gcagauucca gaaacaaauc aaauguuuau guaguuaaag gagucgcaac agcggaguuc     2520

aggaaaaugu ggggauuaca aucggaauac gaaaagaaau gcagagacaa ccauucccac     2580

cacugcaugg acgcaauuac aauugccugc auagguaaaa gagaauauga ucuaauggca     2640

gaauacuauc gcauggaaga aacauuuaga caaggcagag guaguaaacc aaaauuuagu     2700

aaacccuggg caacauucac agaagaugua uuaaacauau auaaaaauuu guuaguagug     2760

caugacacuc caaauaauau gccuaaacau acaaagaaau auguucaaac cucaaaagga     2820

aaaguucuag cacaaggcga cacagcuaga gguuccuuac aucucgauac auacuacgga     2880

gcaauagaaa gggacggcga aauacgauau gucguuagaa gaccauuguc aucuuucaca     2940

aaaccaaaag aauuagaaaa uauuguugau gaaacgguaa aaagaacaau aaaagaagca     3000

aucgcagaca aaaauuuuaa acaagcaauc gccgaaccua uuuauaugaa ugaagacaag     3060

ggaauacuaa uaaagaaagu acgaugcuuc gcuaaauccg uaaaacaacc aaucaauaua     3120

cgacaacauc gagauuuaag caagaaagaa uacaaacaac aauaucaugu cguuaacgaa     3180

aacaacuaca ugcuagucau cuacgaaggu cuuguuaaaa acaaaguagu acgagaguuu     3240

gaaauagucu cauacaucga agccgcaaaa uauuauaaac gcagucaaga ucgaaacaua     3300

uucuccucca ucguuccaac acacucagca aaauacggac ucccacuaaa aacaaagcug     3360

cuaauggggc agcuuguacu gauguucgaa gaaaacccag augaaauaca agucgauaac     3420

acuaaagauc uaguaaagcg cuuauacaaa guaguaggua uaaagaaaga ugggagagug     3480

aaauucaaau uccaccaaga agcaaggaaa gacggacugu uaauauucuc aaccccuuau     3540

aaaaacaaua acggauacgu uccaauauuu agacauagca agaaaaacau aaacauacuu     3600

guagacggaa aagacuucac uauagauaua cuuggaaaag uaacauuaaa agaauga        3657


<210>  5210
<211>  3660
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5210
augaaagaag aaguacgagg caaaaaccgg agccagacau gggcaauuuu agcaaaauuc       60

uuugugugga gugaaaaaga aagugugugg aacgaaaaag aagcgagaac ugaagaacga      120

guaaaagaau acaagcuagu gggauuaaaa agagauacaa aagguaacga auugaaacua      180

gaaaauuauc guuggagagu acaagcccua ucagaacaac uaaaucccga aaucguagcc      240

auaguacuuc aagaaauaaa uggacaaaua ucagcaucau caggguaccu gggcgcuauc      300

ucugaccgaa gcaaacaacu uuauuuuaau agacaaacag uaggacaaua ccuaaugagu      360

gaacuugaga aagacccuaa cguaaguuug aggaaucgag ucuuuuacag acaagauuau      420

uuagacgagu uugacagaau augggaaaaa caagcagaau uucaugaaga auuaacugua      480

gagcuuaaga aagagauaag agacauuaua auauuuuauc agcgucgacu uaaaagccaa      540

aaaggauuaa uaucauuuug ugaguuugaa aaacgcgaag ucgugguaga agaagaugga      600

cgaaagaaaa auaaaaccgu gggaugucgc guaauaccaa gaagccaucc ccuguuccaa      660

gaguuuaaaa uauggcaaac acuaaacgac auagagguau uugcguggga uaaacaauca      720

aaaagaaaga aagcagacaa aaguucaaua uuauuugaca auucagaaga ugcacuauua      780

guagaaggaa aaagauuccu auaucaagaa gaaaaagaac uucuagcaaa agaguuauuu      840

guaaaagaau cuaugaagaa agcugaagug cuaaaacuac uuuuugaaaa cuaucaagaa      900

cuagacuuga acuuuaaaca gauagacgga aaccacacag guuuugcacu auuuagugca      960

uauucaaaaa ugauagaaaa auacggauac gaaccagugg acuuuaaaag aucagcagau     1020

gaaauaauag aaaaacuuga aacaaucuuc acaaacuuag guuggaauac agaacuuuua     1080

ucaauugacc ugagcaaaga agacaaagaa cuugaaaggc agucauacuu cagauuaugg     1140

caucuauugu auucauucga aggggauaac accccaaccg ggaauggaaa acuauuagag     1200

aaaauaaugc aacuuuguga uguugaaaaa gaauaugcgg uggaacucgc uaauguuuca     1260

uuccaggaag auuauggguc ccucucagcg aaagcaauaa agaaaauccu accauacuua     1320

aaagaaggca aucaauacga uguagcgugc gacuacgcag gauauagaca uucaagaagu     1380

ucacuaacaa aagacgaaau agaaaauaaa auauuaaaag auaaacuaga aauauuaccg     1440

aaaaacucac ucagaaaccc aguuguugag aaaauauuaa aucaaauggu aaacuugaua     1500

aacacguuga uugauacaua cggaaaaccu gaugaaauaa gaguugaacu ugcacgugaa     1560

cuaaagaaaa augcuaaaga aagagaagaa cuaacaaaau caauagccag aaauacacgc     1620

gaacacgacg aaauaagaca acuacuaaga acagaauuug gcaugaugaa uguuagcagg     1680

aacgacauca uacgauacaa acuuuaugaa gaacucaaag auaauggaua uaaaacauua     1740

uauaguaaug aauacauacc aagagagaaa auauuuucaa aagaaauuga cauagaacau     1800

guuauccccc aggcccgauu auuugacgac ucuuuaucaa acaaaacacu cgaauaucgu     1860

gcaauaaaca uagaaaaagg aaacaaaaca gcauacgauu uugugaaaga aaaauauggc     1920

aaugauggcu uagaaaaauu ucugaacaga ugugaaacac uauucaaaga uaaaagaacu     1980

aaacucagaa aauuaaaaau ggaagaaaaa gacaucccag aaggauucau cgacagagau     2040

cuccgaaaca cacaguauau aucaaagaag gcauucgcga ugcucaauga aaucagccgg     2100

aggguaguag caacaacagg aaguauuaca gauaaacuaa gagaagacug gcagcuagug     2160

gaccuuauga aagaauuaaa cuuaccgaaa uacgaaaaau uagggauggu cgaaauguuu     2220

gaagauaaag acggaagaaa aauaaagaaa auuacagauu ggacgaaaag aaacgaucau     2280

agacaccacg cagucgacgc acuaacggua gccuuuacca aagacguauu uauucaauuu     2340

uauaauaaua aaaacgcagc auggaugcca gcguccaaag aacaugcaaa caucacaggu     2400

auuaaggccc gauacuuuga gaacgguaaa gcucuagccc caaugccauu agcucaauuc     2460

cgggcagaag cuaaaagaca ccuagaaaau uuauuaguau cuauuaaagc gaaaaauaaa     2520

guuauaacau caaacguaaa cagaacgaaa aacaaaaaug gcgagaaccg aaaauuacaa     2580

caaacaccac gcggucaauu acaucucgaa acuguauaug gaagucacag acaauacgca     2640

acaaaaauag aaaagguaaa ugcagccuuu gauguuacga aaauagcuac agugagcaaa     2700

cuagccuaca gaaaugcacu auuaaaacga uuagaagcau uuggaaacga cccuaaaaag     2760

gcuuuuacag gcaaaaacac acuagagaaa aacccauugu acuuggacga aaaccaaaca     2820

gaaaugguac ccgaaaaagu gcaaacggua gaguucgaaa ccguguacac aauaagaaaa     2880

ccaauagacc cuaaccuguc aaucgauaaa guaguagaug ucaaaauacg uagcguucua     2940

gagaaaagac uaaaagaaua uggaggggac gccaagaaag cguucguaaa ucuagaugaa     3000

aacccgauau gguuaaauga agcgaaagga auuagcauaa aacgaguuuc aauacgaggc     3060

auuuccaacg cccaaucuuu acaugaaaag aaagacaaag acgguaaccu aauacuaggg     3120

aaagaaggaa gaaagauucc agucgacuuc gucaacacgg gaaacaacca ucauguugcu     3180

auauacaaaa agcccgucuu agacaaggug ggacaaguag ucuuagacga agaaggaaac     3240

ccgaaauaug aacuagacga aguaguugua ucuuucuucg acgcaguaac aagagccaac     3300

cugggccaac caauaauaga uaaggauuac cgccgaucgg aaggcuggca auucuuauuc     3360

ucaaugaaac agaacgaaua uuuuguguuc ccaaaugaca aaacaggauu uaacccaaaa     3420

gaaguagauc uacugaaucc agaaaacuau gcucuaaucu cgccaaacuu auucagagua     3480

caaaaauuug cguauaaaaa uuacgucuuu agacaucauc uugaaacgac cauaaaagau     3540

accagcucca cuuugaaagg aauaaccugg accgacuuua gaucgaguaa aggauuagau     3600

aaaaucguaa aggucagagu cgaccacaua ggacaaauag uaucaaucgg ugaauacuga     3660


<210>  5211
<211>  3663
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5211
augaaaagaa uccuaggccu cgacuuaggg ucugcaucaa uuggaugggc ucuaauugag       60

gaagaugaaa aagguauaag aaucauagcc uuaggaucac gaauaauacc uuauaacgga      120

acagaaggcc aagacuucgc aaaaggugcg ggugaaagua gaaacucacu gagaacccaa      180

gcaagaacca caaggaaagg cuacgauaga uaucaguuaa gacgaaaaua ccugauaaau      240

guucuagaga aaaaucuaau gaugccgagc gaagaccuaa gaaaaaugcc caaaaugaac      300

cuauggcaac uaaggucuaa agcagucaca gaauauauau cuaggcaaga acuuggaaga      360

cuacuauugu gguugaauca gaagagaggu uauaaaaguu cacguuccga cgcaaaccuu      420

gacaagaaag auacugacua cgucaaagaa guaaaaucaa gauuugauaa gauaaaggag      480

aaaaaucaaa ccauaggaca auauuuuuac gaaagucuuc aaugcgacga auacuucaga      540

guaaaggaaa acguuuuucc aagagaagca uacauagcgg aauuugaugc aauauguagc      600

agacaaaaag aucaucuagg acuaagugaa gaacucauug cugaaauaag gaaugaaauu      660

auauacuauc aaaggccauu aaaaagccaa aaaggucucg uaucagugua ugauuucgaa      720

ggcaaaugga ugaaccaaaa uggaaaagaa uuauucauag gaccaaaggu agcgcacaaa      780

ucauccccau uauuucaacu agcuaaaauc ugggaaagug uuaauaauau uaaauuaagu      840

acaaaauaug gugauacaau agaguuaaca cucgaagaga agaaacagcu agucgaguuc      900

cuagauaaca acgaaaaacu uacaacggca gcacucuuua aaauauugag gaaagauaag      960

aaagaauaua uacuuccaaa acaacuggaa aaaggacuac aaggaaacau aacgaaaugu     1020

aaaauagcug auauucuagg cucgaaauau aaucaacuau uacaacuaaa ucuagaaaaa     1080

caagaaguag gaaccccaug uuaccuauac acaaaaucaa caggagaggu auuaggagaa     1140

aaacccuugc aauauaucga uaaaagcguu gaaaaagagu uauuuuacca gcuauggcac     1200

accaucuaua gcauaucaga ugaagaugaa ugcuguaaag ccuuacagaa aagguucgac     1260

auagaugcag aaacagcagg cagacuagcc aaaauugacu ucucaaaaua uggcuucggc     1320

aauaaaagcg caaaagcaau ccgaaaaaua cuaccauauc uuauggaagg agacaaauac     1380

agugaugcaa uguguuacgc cggcuauaac cacagcaauu cauuaaccaa agaagaaaac     1440

caugaacgaa aacuaaacga uaagcuaaaa uuacuaacua aaaacuccuu gagacaaccc     1500

auaguugaga aaauaaugaa ccaaaugaua caauuaguaa auacaauaau agauaaauac     1560

gggaagccag acgaaauaag auuagaacuc gcacgagaac ugaaacaauc uaaagaagaa     1620

aggaacgaug cagacaaagc uaugagcaaa cggcaaagag aaaacaaaga ggcagagaag     1680

aaacuagaag aacaugguau acgugccaca cauaacaaua uauuaaaaua uaggcuauuu     1740

gaagaaaugg gggagaagaa acacgaagca auauguauuu acugcggaaa ucccauuagu     1800

uuuucagcag caaugaacgg ugacgaaguc gacguagaac auauaauccc aagaucaaaa     1860

cuuuuugacg auucucagag uaacaaaaca cuagcccaca gaaaauguaa ugcaaacaaa     1920

aacgauauga cagcuuacga cuuuaugaaa accaaaucca aacaagacuu ugacaaauau     1980

guagaaaggg uuaaucaauu auacgcggau agaaucauca gucguaguaa auacaacaaa     2040

uuacuuauga gcgaagauga uauaccaagu aauuucauug acagacaauu aagagaauca     2100

caauacauag caacaaaagc ccgagaaauu uuacaaacaa ucugcuacaa cguaugguca     2160

acaaguggaa caguuacggc agaacuacgc cacuuauggg gcugggauga uauuacaaug     2220

aauuuacaaa ugccaaaaua uaaagaauua ggacugacag aaaugguaga auggacuucu     2280

gaccauggca aaaaugucca cucaaaagaa guaaucaaag gauggaccaa acgggacgac     2340

cacagacacc acgcuauaga ugcccugacc auagcuugua ccaaacaggg uuucauacaa     2400

cgauuuaaua cauuaucauc gucaaaaaca agagaugaca ugcaaaaaga acuagaaaaa     2460

uguucaauac aauucagaga aaaacgaucg uuacuugaaa aauacauaau cagucaaaga     2520

ccaauaagcg uaucagaugu agcugaagca guaucacgca uucuaauuag cuucaaagca     2580

ggaaagaaag uagcaacuau aggaaaaaga aaagucaaag gcaaaguagu acaacaagga     2640

auaguaguac caagaggagc ucuuucugaa gggagcguau acggaaagau aagaacuaua     2700

guaaggaaaa ugccagucaa auauuuauuu gaaaauccag acuuaauauu uaaaccauau     2760

auaaaggcuu uagucaaaga aagauuagca gaguucgaua augacaacaa gcaagcucuu     2820

gcaucauuaa agaaaaaucc cguauaccuu gacaaagaga aaacaaaacu ucuagaauac     2880

ggaacuugcu ucaaagauga auacguuaaa aaguaugacg ucaacccuaa cuucacaaaa     2940

auagcugaug ucauagacgg aaaagucaaa caaauacuag aagauagauu aagcaaauuc     3000

ggaggaaaac ccaaagaagc auucaaggac guaguaaaag acgggaaaac acugaagugg     3060

uaugaagacg aaggguuaga aagaccgauc agaaguguac guuguuuaac gggccucagu     3120

gccguuguac caguaaagaa agaugaaaac ggaauggaca uaggauuugu caaaccaggc     3180

aauaaccacc acguagcuau uuacguagga gcagacggaa aaggacaaga acauguaugc     3240

acauuuugga augcagucga aagaaagaaa uauggauuac ccaucaucau aaaagaugcc     3300

aaugaaguau gggauaaaau uacaauggcc ggugaugaug uuuauccaca aucauucuua     3360

ucucuauuac caaaaccaga uuggacuuua aaacuaucaa ugcaacaaaa cgaaauguuc     3420

aucuuaggaa uggaagaaga ugagguaaaa uuugcgauug aaucgaaaga uuacaaauua     3480

uuaucugauc accuauacag aguccaaaaa cuaucaucaa gugacuacaa uuucauccac     3540

caccuugaaa cagacguaca aaagaaaaca augacguaua gaguaagguc acuaucaggc     3600

uuauucgcgc uaagaccauu uaaagugaga aucgacugcc uaggaaacau caucccaagc     3660

uga                                                                   3663


<210>  5212
<211>  3669
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5212
augaagaaaa gaauacuugg ucuagauuca ggaacaaauu cacuagguug ggcagucgua       60

gaccgagaug agaacaauca auacagcuua auaaaauacg gagaucuaau auuugaagaa      120

ggaguuaaaa uugaaaaagg aguagaaagu ucuaaagcag cagaacgaac gagccaccga      180

gccuugagaa aacuauauuu ucguagaaga uuaagaaaaa uagaagugcu aaaaguucug      240

guaaaauaca accuuugccc ucaaguauca gaagaagaau uacauuuaug gcacacaaga      300

aaacuauauc cacaaaauaa agaccuucuu caauggcaaa gaacugacga aaaagcugga      360

aagaacccau auauauacag auauagaugc uuacaugaag aauuagauau gucaguaagu      420

gcuaacagaa ugacaguugg cagagccaug uaucaucucg cacaacgaag aggauuucua      480

ucaaacagac uagauaacga agaagacgaa aaccaaaucg gagcaguaaa augugguauc      540

ucaaaccuua ccgaagagau acaaucaagu ggauguaaau acuugggaga auauuuuuau      600

aaacucuaua augaacaugg aaacacggug cgucuaagaa cacgauauac uucuagagag      660

uuccauuaua agaaagaguu cuaugcgaua ugugaaaaac aaaaauuaga uaagaaauua      720

auagaagaau uagcaagugc ccuuuacuuu caaagaccau uaaaaucaca aagacacacg      780

guaggacgau gcacuuuuga accaaacaaa ccaagaugcg ccgauucaca ccccgcauau      840

gaagaauuua ggauguuauc auucauaaau acaauaaaag uacaaggacc uuacgauacc      900

gaacuaagag uacuaaacac agaagaacga gacucaauaa cuaaacuauu uuguagaaaa      960

agcaaaccaa auuucgauuu cgaagacauu gccaaagaac ucgcaggaag aaauaauuau     1020

uguuauauag gcgaugucgu agacaaggcu uacaaauuua acuacaggau gucacaagga     1080

gucagcggau gcccuacuac cacuuaucua auaaacgugu ucggccaaga cuggcaaaca     1140

ggaauagcag aaucuuacca agcaauugau acuaaauuag gccaaaaauc uguagaugcc     1200

augaucgaug auguauggaa cgugcucuau aguuucaguu cgaaagacaa auuaagagau     1260

uucggacgua agaaauuaca guuaggagau aaagaggcag acacguuugc gaauauaaaa     1320

cuuacacaca aauuugcuag ucuuucauug aaagcaauac gaaaaaucaa uaucuuccua     1380

agaaugggcc uaguauacuc acaugcugua acacuagcaa auaucccgac aauaguuggc     1440

acugauauau ggcaaagaaa aaggaauuuc auacuagcag aauugcuucc auuaauagaa     1500

aauuucaauc caaaagaaac aaaacuacaa gaaacacugg auuuuugugu aaaagauuuu     1560

cuacaaaaua acuuugaacu caaaaacggu gcuguagaca aacuauauca cccuaguaug     1620

aucgaggcau auccggaugc agguuuaaac ucaaacggaa uacuccaacu aggaucucca     1680

aggacaaaug caauucgaaa ucccauggca augcgaaguu uacaugaaau uagaaaagua     1740

aucaaucaac uacuaaaaga uaaaauaaua aaccaagaca cugaaauaca cguggaauau     1800

gcaagagaau uaaaugaugc gaacaaaaga aaagcaauaa gcauauggca aaaaucccag     1860

gaaaaacuac auaagaaaua uguagaagaa auuacaaacu uauacaaaga agagaccggu     1920

aaagcuauag ugcccacgga cacagauauc cucaaauuua aacucuggga ggaacagaau     1980

cacauuugcu uauauacagg acaacaaauc ggcauagcug aauuuuuagg ugcgacaccg     2040

aaauacgaca uugaacauac aaucccacag agcguaggcg gagauucgac agauaugaac     2100

cugacauuau gcgauaguag auucaacaga gaaauuaaga aagcucaauu gccgacucaa     2160

cuaggaaauc acgcagauau ccuagucagg auagcaggau ggaaaaauuc cuaugaaaaa     2220

cuaucuaaag acauagauag aauaagaaca cacucaggaa uggcuaaaga uauuaaagac     2280

aggcucaucc aaaaacggca ucuuuuaaaa cuugaaagag auuacuggag aggaaaauac     2340

gaacgguuca caaugaacga aauucccgaa ggauucagca gaagacaagg ugugggcaua     2400

ggacucauau caaaguacgc aggucuauau cuaaaaaguc ucuuucacga uaaaaaugaa     2460

aggaauaaag gacacgucuu uguugucaaa gguacaacga cagcggaauu ucguaaaaug     2520

uggggauuac aaaacgaaua cgagaaaaag uccagaaaca aucacguaca ccacugcaua     2580

gaugcaauca caauugcaug caucggaaaa caugaauacg cccagauggc gcaauauuac     2640

cacgaugaag cagaauacuu auaccaccau ggaauaaagc caaucuuucc gaaaccaugg     2700

aaaacauuua cagaagacuu acuggaccua gaacauagcu uauuuguggu acaccauagu     2760

ccuaacaaua uggcuaaaca agcaagaaaa agaauaauaa ccagcacagg acaacauugg     2820

gcacaaggag acucagcaca uggcagucua cacaacgaua cuuauuaugg ugcaaucgaa     2880

cgcgauggag agauaaaaua cgucguaagg agagccguaa gcucauuuga acaaaugagu     2940

gacgucgaua guauaguaga ugacgucgua aaagaaacag uaagaaaagc acuagaaggc     3000

aaaaacuuua aagaagccgu gaaugcacca auauauauga auaaggaaaa aggaguccua     3060

auaaagaaag ucagaugcua ugcaaagagu auuaaaaagc ccauagacau aagaacucac     3120

agagauucau caucaaaaga guauaagcga aaauuccaug uaucgaacga uauaaacuac     3180

cuaauagcaa uauacgaagg aacgguuaaa ggaaaaguaa aaagagacuu ugaagucguc     3240

aaaaacauag aagcggcaaa cuucuuuaaa agaucacaag auagacgcga uuacccgaca     3300

auaguuccag aaaagaguau aaaacacgga uucccuuuaa aaacaacacu aacaaaugga     3360

accaugguga uuuuguacga agagaauaaa gaagagauag acuuuacaaa gaaagaagac     3420

auauguagaa gacuguauaa auuaguagga augucuucaa ugauaguuag uggaugugaa     3480

uauggaguac uaagccuacg ccaccaccaa gaagcaagac caucaaaaga uauaaaaagc     3540

aaaaacggag ccuucaaaaa uggcgaagcc uuacgaccgg cgauuacuau gcuucacaca     3600

caauucaacg cgcuagucga aaacauugac uuuaauauag auguaacagg gaacauagaa     3660

uugauauga                                                             3669


<210>  5213
<211>  3669
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5213
augaagaaac gaauacuagg ccuagacuca ggaaccaacu cauuaggaug ggcuguuguc       60

gauagagaug aaaacaauca auauucucua auuaaauaug gggacuuaau auucgaagaa      120

ggagucaaaa uagaaaaagg aguagaaagc ucaaaagcag cagaaagaac cucacauaga      180

gcauuacgaa aacuguacuu uagaagaaga cuaagaaaaa uagaagugcu caaagucuua      240

guaaaauaca acuuaugccc acaaguaucc gaagaagaau uacacuuaug gcacacacga      300

aaacuauauc cacaaaacaa agaccugcua caauggcaaa ggacagacga aaaagagggc      360

aaaaauccau acauuuaccg auaccgcugc uuacacgaag aacuagacau gucugucucg      420

gccaacagaa ugacggucgg aagggcaaug uaccauuugg cacaacggag aggauuccuc      480

agcaaucgac uugacaauga agaagaugaa aaccaaauag gagcgguaaa augugguauc      540

uccaaucuua ccgaagaaau ccaaucguca ggauguaaau accucggaga auacuuuuau      600

aaacuuuaua augaacacgg aaauacagug agacuccgaa cccgauauac guccagggaa      660

uuucacuaua agaaagaguu cuaugcaaua ugugaaaaac aaaaauuaga uaagaaacuc      720

auagaggaac uagcaagugc cuuauacuuc caaagaccac ucaaaaguca acgacauaca      780

guuggucgcu guacauuuga gccaaauaaa ccaaggugcg ccgacucaca uccugcuuac      840

gaagaguucc guaugcucuc guucauaaac acuauaaaag uucaaggacc guaugauaca      900

gaauuaagag uauugaauac agaagaacga gacucuauaa caaaauuauu cugucgaaaa      960

ucaaaaccua acuucgacuu ugaagacaua gccaaagaau uagcagguag aaauaauuau     1020

uguuauauag gagauguagu agacaaagcg uauaaauuca acuauagaau gagccaagga     1080

guaucaggau gcccaacuac uacauaucuu aucaacguuu ucgggcaaga cuggcagaca     1140

ggcauagccg aaucauauca agcgauagac acgaaacuag gacaaaaauc cguggacgcc     1200

augauugaug auguauggaa uguguuauau uccuuuagcu cuaaagauaa auugagggac     1260

uucgguagaa agaaauuaca acuaggagac aaagaagcag auacuuuugc caacauaaaa     1320

cuuacacaca aauucgccag cuuaucuuua aaagcaauaa gaaaaaucaa uauauuccua     1380

agaauggggc uaguauacag ccacgcagua acuuuagcaa acauuccaac aauuguagga     1440

acagauauau ggcaacggaa aagaaauuuu auauuagcag aacuauuacc acuaauagaa     1500

aacuuuaauc ccaaagaaac aaaauuacaa gaaacguuag auuucugcgu caaagacuuu     1560

uuacagaaua acuucgaacu uaaaaacgga gccguagaca aauuauauca uccaagcaug     1620

auagaagcau auccagaugc aggcuuaaau ucuaauggga uucuacaauu agguagccca     1680

cguacuaacg ccauucgcaa uccaauggca augagaaguc uacacgaaau aagaaaaguc     1740

auaaaccagc uauuaaaaga uaaaauaauc aaccaagaca cugaaauaca ugucgaauau     1800

gccagagaau uaaaugacgc aaauaaaaga aaagccaucu caauauggca aaaaucacaa     1860

gagaaauuac auaagaaaua cguagaagaa auaacaaauu uauacaaaga agaaacagga     1920

aaagccauag uacccaccga uaccgauauu uuaaaauuca aauuauggga agaacaaaau     1980

cacauauguc uauauacugg ucaacaaaua ggaauagcgg aauuucuagg agcuacuccc     2040

aaauacgaua uagaacauac cauaccacaa agugucggag gcgauucaac agacaugaac     2100

uugacccuuu gcgacucgag auucaaucgu gaaauaaaga aagcacaacu accgacacaa     2160

cuuggaaacc acgcagacau ccucguaaga auagcaggau ggaaaaauuc guacgaaaaa     2220

uuaaguaaag auauagacag gauaagaaca cauagcggua uggcuaaaga uaucaaagac     2280

agacugauuc agaaaaggca cuuacucaaa cuugaacgag auuacuggag aggaaaauau     2340

gagagguuua cgaugaauga aauccccgag gguuuuucca gaaggcaagg ggugggcauu     2400

ggacucauuu caaaauaugc ugguuuauac uuaaaaagcc uauuucacga uaaaaacgaa     2460

cgaaauaaag gacacgucuu cguagucaaa ggaacaacaa cagcugaauu uagaaaaaug     2520

uggggacuac aaaacgaaua ugaaaagaaa agccgaaaua accacguuca ccacugcauc     2580

gaugccauaa caauagcaug uaucgguaaa cacgaauaug cacaaauggc acaauacuac     2640

caugacgaag ccgaauacuu auaucaccac ggaaucaaac caauauuccc aaaaccaugg     2700

aaaacuuuca ccgaagaucu auuagacuua gaacacagcc ucuuugucgu acaccacucu     2760

ccuaauaaua uggcgaaaca agcgaggaaa aggaucauaa cuucuacugg ucagcacugg     2820

gcucaaggug auucggccca uggcucguua cacaaugaua cguauuaugg cgcaaucgaa     2880

cgggauggag aaauaaaaua uguaguaagg agagcaguau ccaguuucga acaaauguca     2940

gauguagaca guaucgucga cgauguagua aaagaaacug uaagaaaagc auuagaaggc     3000

aaaaauuuua aagaagcggu aaaugcacca auauacauga auaaagaaaa aggaguacuu     3060

aucaagaaag uuagaugcua ugccaaaagc auaaagaaac caauagauau acgcacgcac     3120

agagacucau ccucaaaaga auauaaacga aaauuucacg ucucaaacga uauaaacuau     3180

uuaauugcaa uuuacgaagg aacaguuaaa ggcaaaguca aaagagauuu cgaaguugug     3240

aaaaacauag aagcagcuaa cuucuuuaaa agaucacaag auagaagaga cuaucccacc     3300

aucguaccgg agaaaagcau uaaacacgga uuuccauuaa aaacaacccu gacuaacgga     3360

acgaugguga uccuauauga agaaaauaag gaagagauag auuuuaccaa gaaagaagac     3420

auaugccgac gacuuuacaa gcuaguaggc augucaucca ugauagucag cggaugcgaa     3480

uacggagugc uaagccuuag acaccaccaa gaggcaagac caaguaaaga uauaaaaagc     3540

aaaaauggag cauucaaaaa uggagaagcg cuacgaccag caauuacgau gcuacauaca     3600

caauucaacg cuuugguaga aaauauugau uuuaacauag augugacagg caacauagag     3660

uuaauauga                                                             3669


<210>  5214
<211>  3678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5214
augagcaaac gaauacucgg aaucgacguu ggaacaaacu cacuuggcug ggcagucgua       60

gauagaacag acugugauaa cuacacucua gugaaaagag gugauguaau auuucaagaa      120

ggcguaaaca uugaaaaagg caacauagaa aaauccagag cuagcgaacg aacuggucau      180

cgaucaacaa ggaaacacua uuuccggcgu agaaugcuaa aaaucaaagu ucuagaaauu      240

uuaguagaac aucagcuuug cccuuauuua ucacacgagg aacugcauca auggaugacc      300

cgcaaaguau acccgacuaa ugaggacuuc agacuauggc uaagaacuaa cgaugccaau      360

gaucaaaauc cauaucauga uagacaugua ugccuacacg aaagauugga ccuaagauua      420

caagaaaaua gguaccgauu aggacgcgca cuauaucauu uagcacaaag aagaggcuuu      480

uuaucuaaua gacuagaaca aaauucaaaa acugaugaaa caggagcagu caaaucagau      540

auuagucaac uuaaugaaga aaugaagaaa gcaggaucac aauacuuagg ugacuacuuu      600

uauuggcucu aucaggaaua cgggaaucag gugacuuuac gaauaagaua cacagauagg      660

aaacaacacu accagaaaga guuugaagcg auuugucaga gacaacaguu gagcgacgau      720

cuuguugaua aacuuagacg agcuauauac uuucaaagau cccuuaaauc acaaagagcc      780

gggguuggaa aauguaccuu ugaaccggau aaaccacgau gcgcuuuaag ucauccaaga      840

uaugaagaau uuagaauguu gagcuuuuua aauaacguga agguuaaagg accaucagau      900

gauuccuuga gaccacuuuc accaagugaa aaagagaaaa uaaaaccccu cuucuuacga      960

aaaaguaaag ccaauuucga cuuugaagac auagcuaagg caauugccgg aaaaggaaaa     1020

uaugcuugua uaagagacaa agaaggugcu caaaagccgu accaauuuaa cuacgcguua     1080

ucacaaggag uaagugggug cccugucacu gccaaacuaa ucgccguauu uggugaauca     1140

ugggaacgaa auuuaguaaa ugcguauaga uccaggaacg auaugcgcaa aucugaugaa     1200

gaaauaguca augaaguuug gaauguacua uauagcuucu cgucgaaaga aaaguuaaga     1260

gcauggggaa uggaaaaacu cggauuagau gaagccugcg caggagaguu cgcugauaua     1320

acacuaccaa cagcauuugc agccuuauca cucaaagcag uuaauaaaau ucuaccauuu     1380

cuacgacaag gauucauaua cucgcaugca guaauguuag caaauauacc auauauaaua     1440

ggaauggaua gauggaacga accgcgacaa agacaguuca uaauggauaa uuuacaggaa     1500

cuauuggaca acuucacggc uagcgauaaa gcauuacaac aaaccuugga cuuuugcaua     1560

aaagacuuuu uauuaaacaa uuaugaacuu gcaccuggga aagccgauag acuauaccac     1620

ccuucuauga uugaaacuua uccagaugca aaagcagacg gucaaggcau cuaccaacua     1680

ggaagcccug cuacgaacgc cauaagaaau ccuauggcua ugagaagucu gcacauacua     1740

cgaagaguug uaaaucaacu auuaaaagaa aagacuauug auagugaaac agaaguacau     1800

auugaauacg cuagagaacu aaacgaugcg aauaggagau gggcaauaaa ucaauggcaa     1860

agaaaacagg aaacacaaag aaaagauuau gcagaaaaga uuagaaaagu cuacgcagau     1920

gaguucgaaa aaguaauaga accaacacaa gaagaacucu uaaaauacca acucugggag     1980

gaacaacaac auuugugucu auauacagga gaacagaucg gauuaacaga cuucaucggg     2040

ccaaauccca gauaugauau agaacauacc aucccacaau cuaugggcgg agauagcaca     2100

cgaaugaauu uaacacugug ugagagaaca uacaauagaa ccguaaaagg aaaccgaaug     2160

ccgucgcaac ucgcagaaca cgagucgaua uuaacacgaa ucaaacccug gaaagacaaa     2220

uacgaagaac uaacagucaa aauaaauaaa uuaagaacac auccaggcau ggauaaagca     2280

ucaaaagaua auauuauaag aaaaagacau uuauuagaau uagagaggga uuacugguuu     2340

caaaaauauc aaagauucac aaugaccgaa guaccugaag guuucucccu acgucaaggc     2400

acaggaauag gacugauugg aaaauaugcu ggauuguuuu uaaaaucucu auuccaucau     2460

guuaaagaac gaaaucgaaa acaaguauau guuauuaaag guccuauaac agcggaguuc     2520

agaagauuau ggggauuaca aaaucucuau gaacagaaaa gcagagacaa ucaugcucac     2580

cauuguauug acgcuaucgu aaucgcaugc auaaacccag cugaauaugc cagaauggca     2640

aguuauuauc gacaagaaga agacuucaga cuaaacaaag gaaauaaacc cguauuccca     2700

aaaccaugga cuacauucac acaagauuua caagcucucg aagccgaauu gcuaguagua     2760

caugaaacga gaaauaaucu aacuaagaaa gcaagaaaac ggguccgaac gaaaggcgga     2820

aacguacuau cagaaaguga cucggcacgc gggucacuac auaacgacac auauuaugga     2880

gccauacaga aagacggugu aauaagauac guaguacgaa aagcauuguc aucauuguca     2940

gaaaaagacg ucaaagauau aguagacaaa acagugagag aaaaaguaca ggcggcgaua     3000

caagagaaag gagcaaaaga agcacuagcu gguaccauau auaugaauga agaaaaagga     3060

auuaccauaa agaaaguuag auguuucaca aaaaucgcua gcccauuggc gauaagaaga     3120

caccgagaug cuucugccaa agaauacaaa cgaccauauu auguaacaaa cgaaaguaau     3180

uaugugaugg guauuuauga gggaugcauu aaagagaaaa caaaaagaga guucauucuc     3240

gugaauaauc uagaagcggu gagacaauua aaagcugcac gaaaacaagg cguugucuca     3300

agcauucugc ccccaguuuc ugcaaaaagu caauuucccu ugaaauauag acuuuauaca     3360

ggcacauugg uacucuuaua ugaaaauuca cccgaagaaa uagauuuuac uaaucaaguu     3420

gacauaugca aaagauuaua uaaaguuaca ggauuaagca gcaugaccgu uacagguaac     3480

caauacggaa caauacaauu acgauaucac caagaagcac ggcaagcaaa agaccuaaaa     3540

gcacaaaacg gagcauacaa agaaggggaa gaacaucgac ccgcaauuau gauguuacau     3600

acccaauuca aagccuuagu agaaggaacc gauuuccgaa ugaauauucu uggugaaauc     3660

gaaccauuaa ggaaauga                                                   3678


<210>  5215
<211>  3690
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5215
augggagacc gcaaauacaa cuuaggauua gauaucggaa caucgucaau cggauucgca       60

gcuguagaug aaaacaacca acccauacga guuaaaggaa aaacggcuau cgguguaagg      120

cuauucgaag aaggcaaaac cgcagcagau agacgcggau ucagaacaac cagaagacgc      180

cugucaagac gaagauggcg aaucaaccuu cuuaaugaaa ucuuugaugc ucaccuggca      240

gaaguggacc cuacauuccu agcaagacuc aaagaaucua accguaguaa ucucgauccu      300

aagaaaucuu uucaaggcag ccuacuauuc ccugaaagga aagauuacca auuuuacgaa      360

gaauacccaa ccauauauca ccuacgaaaa gcacuaaugg aaaaagauag aaaauucgac      420

auuagagaaa uauaucuagc aguacaccau auaauaaaau accgagguaa uuuccuaaau      480

gggacuccca ugagaucauu uaaaguugaa aacauagagc uaaacacacu auuugaucaa      540

cuuaaucaac uuuacgcgga aaucguaccc gacaaugagu uagcauucga ucuagcucaa      600

guugcugacg uaaaagacgu ucuaaguuca accacaaucu acaaaaugga caagaagaaa      660

caacuuguaa aaaugaugcu acuacccgcu ucaaacaaag cccuacaauc agaaaacaaa      720

aagauuguaa cacaauucgu caacgcaaua cuaaauuaca aauucaaauu agacguauua      780

cuccaaguag aaacagaugc agauuggagu cuuaaauuaa acgacgaagg ugcugacgau      840

aaacuugaag aauuuaccgg agaucuagac gaaaaucgau uagagauaau ugacuuauua      900

caaagacuac auaacugguu uucgcuaaau gaaauaacca aagacggaaa cucacuaagu      960

gccgcaaugg ucgaaaagua cgaaaaucac caucaucacu ugggauuacu uaagaaagua     1020

auagaaaacc auccugaugc aaagaaagca aaagcucuua aagaaaccua uacagccuac     1080

guaggaaaaa cagacgauaa aacgcaaaau caagaugacu ucuacaaagc aguagagaaa     1140

aacuuagaug acaguccuga cgcgaaagga auaaaaagac ucauacaacu agaucaguuc     1200

augccuaaac aaagaacugg ucaaaaugga gccauucccc accaacuuca ccaacaagaa     1260

uuagaucaaa uaaucgaaaa acagucaaaa uacuauccau uucucgcaga accgaaucca     1320

aacguuaaaa gaagaaaaga cgccccauac aaacuagaug aacuuauugc cuucaaaauu     1380

ccuuauuacg uuggaccuuu aguuacgccc gaagaacaag cccaaaauaa agaaaauguu     1440

uuugccugga ugaaaagaaa agcugccggu ccaauuaccc cauggaacuu ugacgaaaaa     1500

guagaccgaa uggagagcgc aaaccgauuu auucgaagga ugacaacuaa agauacauac     1560

uuauucggcg aagacguccu gcccgcugaa aguaugauau aucaaaaauu uguaguguua     1620

aaugaacuaa acaaucuaaa aaucaacggg cgacaucugu cauuaaaaga uaaacaagau     1680

guauauaaug accuuuuuaa acaacagaaa acaguaagca ucaaagcucu acaaaacuac     1740

uauguaacca aaaagaaagc agcuacagca ccaacaguag gaggauuagc agaucccaag     1800

aaauucuuau caucauuaag uacauacaua gacuuuaaaa acauguuugg cgaacgggua     1860

aaugaucccc aauuccaaga agaccuagaa caaauuguag aauggaguac aauauucgaa     1920

gacagaggua uauucaaagc aaaacuacaa gccuuagguu gguuaucuga aaaacaaaua     1980

caacagcuag uagccaaaag auacaaagga ugggguagac ucucuaagaa acuauuaaca     2040

ggacugaaaa acgcugaggg uuauagcauc uuggacgaaa uguggcgauc cacagaaaau     2100

uucaugcaaa uacaaagcag accagaguuc gcagcccuga uacaacaagc aaacgaaaaa     2160

caauuugaag gaaaugaucc agauaacgua ugggaaaaua ucgaaaauau auuaggcgac     2220

gcauacacau caccacaaaa caagaaagcg auacgccaag ugguuaaagu aguacaagau     2280

aucgaaaaag caguaggaaa cccuccugag aaaauagcga uagaguucac aagagaagcu     2340

gcagcaaacc cccaaagaac gcaaucacgu cuccggaccu uagaaaaacu cuaugaaagc     2400

gccgaagaag ucguagacgc cggccuaacc gcagaacuag cagaguucaa agaaaacaaa     2460

cacguauuau ccgacaaaua cuacuuauac uucacgcaac uaggaagaga uguauacaca     2520

ggagauacaa uaucauuaga uaaacuaaac gacuacgacg uugaucauau acucccucag     2580

agcuuuauaa aagacgauuc ucuagacaac agaguacuaa cgauacgagc aguaaauaac     2640

gguaaaagug auaacguacc cgcuaaaaug uuuggcaaga aaaugggguc cuuuuggaga     2700

caccucuugg acaauggaau gauaaguaaa aggaaauaca auaaucucau cacagauccc     2760

gauaacauau caaaauaugc ccaaaaaggu uucauaaaua gacaauuagu agaaacgagu     2820

caaguuauca aacucacagc uaacauauua aacggaaucu acgauaaaga caccgaaauc     2880

auagaagucc cugcgaaaau gaauagucaa augagaaaaa uguuugacuu agucaaagua     2940

agagaaguaa acgacuacca ccaugcauuc gacgcauacc uaacaauauu uauaggcaau     3000

uauuuauaca aaugcuaucc aaaacuucgc cccuacuuug uauacgacaa cuuuaagaaa     3060

uucggcaaua aagaagauau cggacauaaa agguuuaacu uucuagguaa aauagaaagg     3120

gaaaagaaag ucguagcucc agaaacuggc gaaauauugu ggucaaaugu agcaccaaau     3180

gaaaccauca aacaaauuaa gaaagucuau aacuauaaau ucaugaucgu uucaagagaa     3240

aucacaacac gaagggcaga acuauuuaac caaacagucu auccgaaaaa cuaccgugga     3300

aaacucauac caaucaaaga agaccguccg acagaccuau auggaggaua caguggaaac     3360

accgaugcuu aucuagccau uguagcauua gaagauaaaa agaaagggaa auauuucaaa     3420

guaguuggua ucccaacacg agcagcugca aaacuggaaa aacuaaaaca acaagacucu     3480

caacaauacc uccaggcccu ccaugaaguu auagcaccac aauuuacuaa aucaacgaag     3540

aaaggaauca agaaaacaga auuugaaauc guacuagaua aagugcacua uagacagcuu     3600

guccaagaug guccaguuaa aaugaugcua ggcagcucga cauacaaaua caaugcaaaa     3660

cagcuaguau uaucagaaaa agcacuguga                                      3690


<210>  5216
<211>  3696
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5216
augauaacgc guauuuuagg ucucgacaca ggaacaaacu cacuagccug ggcuguagua       60

gaucgcgaug cuaacggaaa uuacacccuc guaaaaagag gugacguaau cuuuacagaa      120

gggguaaaaa uagagaaagg uaucgaaagu aguaaagcag cugagagaac agcacauaaa      180

gcacuaagag uccauuauuc acgcagaaga uuacacaaaa ucgacacuuu aaaaguacuc      240

guagaacucg accuaugucc auacuuaagu aacgaacaac ugaaccucug gaaagcaaag      300

aaaacauauc cauuaacaga ugauuucaug uuauggcaaa gaucaggaag ugcggaagaa      360

aauaauccau auuacugcag acacagaugc uuaacccaaa aauuagauuu aacagaucaa      420

ucacaaagau uucuucuagg gcgagccuuc uaucacuuag cacaaagacg aggauuccua      480

ucgaauagac ucgauacguc agcagacuca gaugagucag gcaaagucaa aaguggaaua      540

gcugaauuau caguagagau ggaaaaagca gggugcgaau uuuuaggaga auacuuuuac      600

caacucuaca aacaacaugg aaacacaaua aaacuaagau caagguauac agauagagag      660

gaacauuaca agaaagaguu ccuggcaaua ugugaaaaac aggaauuaga ucccaaaugg      720

guugaagccu uacaacgugc aaucuauuuc cagagaccac uaaaaaguca acgaaaaggg      780

guuggcaaau guacuuucga accaaagaaa ccuaggugcg cagaauccca uccagauuau      840

gaagcauuua gaauguugug uuucuuaaau aaugucaaaa uucaaacacc aaacgauacu      900

ucccuuagac cauugaacga agaugaaaga gagaaaauau ggccccucuu uuucagaaaa      960

agcaaaccaa acuucgacuu ugaagauaua gcaaaagcau uggcugguaa aaacaauuac     1020

gcaugguaca aagaugcagg cgaaaagcca uuuaaauuua acuacagaau gcaacaagga     1080

guaucuggau gcccaguaac cgcgcaacua auugacauau uuggaaguga uuggcaaacc     1140

gcgauagccg aaacguacac agcaguagaa aagaaaaacg gcacaaaaac aauugaugaa     1200

auggccgaug auguauggaa cguccuguac aguuuuagua guaaagauaa auuaaaagca     1260

uucgcaacug aaaaauuaca acuugacgaa gcaucagcua caaaauucgc caacgcaaaa     1320

uuaccacaug gauuugccuc ccuaagccua aaggcuauca gaaauauauu accauuucua     1380

agacaaggcu ucauuuacgc ucacgcagua cauuuagcga agauaccaga caucgucaca     1440

aaggcgguuu ggaacgacgc agacaaacga gaaaugauac uaucuggguu aauggaaauu     1500

auagaaaacu auaauccaaa agaccgaggg gucgaaggaa ccauugacuu cuguaucaaa     1560

ucccaucuac aggacguuau ugauuuaaga ccaggcgcag cagacgcccu auaucauccc     1620

ucaaugaucg aaacauaccc agaugcuaaa uugaacaaaa ugggagucuu ccaacuagga     1680

ucaccaagaa caaaugcgau acguaaccca auggcaauga gaucauuaca cauacuacgg     1740

ucagucguca aucaguuacu aagggauggu auaauagaua gaaacacaga aguucaugua     1800

gaauacgcaa gagaauuaaa ugacgcaaac aaaagaaaag caauagcaga uuaccaaaga     1860

gaacaggaaa agaaaagaaa acaauacaga gacgauauaa ucgcucuaua uaaaagcgaa     1920

ugucauaaag aaauagaacc aacagacacc gacauauuaa aauuucaauu augggaagaa     1980

caagaucacc uaugccuaua cacggguacu cagauaggaa uaugcgacuu uuuaggcgau     2040

aauccaaaau ucgauaucga gcacacugua ccaagaucag uagguggaga cuccacacaa     2100

augaaucuua cacuauguga cgccacauuc aauagacaag ucaaaggugc ucaacuaccu     2160

ucgcaacuga guaaucacgc agacauccua gucagaauug aucacuggaa aaguaaaugu     2220

gaagaccuua gaaaacaauu agauaaaauu agaacauaug gaggcaugga aaaagcaaug     2280

aaagauagac uuauacagaa aaggcaucga cuaaaaaugg acuuagauua cuggcaagga     2340

aaauacucaa gauuuacuau gacagaagua ccagaaggau ucaguagacg ucaaggggca     2400

gguauaggcc uaguguccaa auacgcagga cuauuccuua aaagucuguu ccacaaugaa     2460

caaaacccga auaagaguaa cguauauguc guaaaaggag uaacaacagc cgaguuccga     2520

agaauguggg gacuacaggg agaauaugaa aagaaaucac gccuaaacca cugccaccac     2580

uguauagacg ccaucacuau agcauguaua ggaaaagcug aauacgauaa aauggcacaa     2640

uacuaucaug acgaagaacu uuuccaaugg agggguacca ccacuaaacc gcacuucaac     2700

aaaccauggc caacauucac cgaagacgua cuugcuaucg aacaaucccu auuaguuaag     2760

caccuaacga aagauaauau gccaaaacaa gcuaagaaaa gaguacguac agcaacggga     2820

aaauaccucg cucaaggaga cacagcacgg ggaucuuugc auaaagauac auacuaugga     2880

gccauagaac aagacggaga aauuagguau guacugagga aaccguuaaa uaaauuugaa     2940

aaugaagcag aauuagagaa aauagucgau caagcgguaa aagagaaaau caaagaagca     3000

auugcaggua aaaauuuuaa aaccgccaua acugaaccua uauucaugaa caaagaaaaa     3060

ggaauacgca uaaauaaagu acgguacuac gucaaagcac aagaaagauu aaagauuagg     3120

caacaacgag accuuagcuc uaaagaauau aaacaauuuu ucugcguaga aaaugaauca     3180

aacuauuugc ucgcgaucua ugaaggagaa cuuaagggaa agaaagaaag agacuuuugu     3240

gucgucaaua auuuagaauc ggcagcuugc cuaaaacgau caaauaaagg gaaagcagca     3300

ccucuagucc cagaaagauc agaagaaaga aaucuuccau uauuagcagu auugaaaaga     3360

ggaacacaag uucuauuuua ugaaaacagc ccagaagaaa uacuauucga caacccacaa     3420

gacuuggugc gaagauuaua caaaguuaca ggcauaaaua caaagaaaga cgucaaaaau     3480

gguaaaacau accuauacgg uaacauugaa cuacgcuucc accaaaaugc acaaaagaaa     3540

gacgauguaa aaaugcaauc aggaguauau aaaaacaaug aaguucuucg ccaaggaauc     3600

guucuaaguc acaaacaguu caaagcccua guagaagggg uagacuucga auuuaaugug     3660

cuaggagaaa uuaaaaagaa agaaagacac gcuuga                               3696


<210>  5217
<211>  3699
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5217
augacuaaaa cacgaauacu aggacuagau acuggaacaa acagucuagg augggcaaua       60

guugaccgag acgaaguaag cggacaauau gaauuaguug accguggaga ucuaaucuuu      120

acugaaggag ugaaaauaga gaaaggcaua gaaucaagua aagcaucuga acgaacgcaa      180

cacagagcuu uaagaaaaca auauuuuaga agacgacuca gaaaaauuga aguacuaaga      240

guucuuaccu uuuaucaccu augcccaccc guuagugaag aagaauuaca ucucuggcac      300

gucaagaaaa uauauccggu cgaaaaccac gaguucauga ucuggcaaag aaccaaugac      360

aaugaaggca aaaacccaua cuacuaucga cauauauguc uacacacaaa acuaaaucua      420

acacuaauga aagacagaua uauucuaggg cgcgcacuau aucaccuauc gcaacgcaga      480

ggcuuucuga guaaucgauu agaucaauca caagacgaag augacaaagg aacagucaaa      540

accggaauaa gcucauuauc aaaagacaug aacgaugcug gaugcacgua uuuaggagac      600

uauuuuuaca aacuauauuc agaaaacgga aauagaguaa gaauacgaaa ccacuacacc      660

gccagaaacg aacacuacuu agcugaauuu gaggcaaucu gccaaguuca aaaauuagaa      720

ccagaauuag uagaagcucu ccggagagca auuuucuuuc aaagaccacu aaaaagucaa      780

cgcaaaggag uaggaaaaug uacauuugaa ccacgaaaac cgagaugcgc agacagccac      840

ccagauuacg aagaauuuag aaaauuaugu uucauuaaua acaucaaaau acaaacgagc      900

gaugacgaca caaugagacc auuaaacaaa gaagaacgac aaagaaucaa uucuuuauuc      960

uacagaguua gcaaagcaaa uuuuuacuuc gaagaaauug cuaaguugcu aggaggucga     1020

aacaacuauu gcuauuuuaa agacgaagua caaaaagacu uuaaauucaa uuaccguaug     1080

ucgcaaagcg ugaguggaug uccuacuaca gcacaacuaa gaaacauauu cggagaagac     1140

uggaaaaccg ggaucgcgga aacauaucag cauuccgcaa agaaagaugg aucauuaaaa     1200

acaguggacg aaauggcaga ugauauaugg aacguguuau auagcuucuc gaguaaagac     1260

ugcuuauaca auuuugcagu aaacaaacua caacuagaag aggauaaagc uaagaaauuu     1320

ucaaaaauua aacucucuca uggauucgcu agcauauccc ucaacgccau ucauaaaaua     1380

cuaccuuucc uucgaauggg aaugauauau agccacgccg uacuauucgc aaacauaccg     1440

acaaucguag gaauggaugu cuggaacgac gaaucaaaac gacaacacau uaugacggaa     1500

uuaagcgauu uaguauucaa uuaugauccc aaaaaccgag aagucgaagg aacaauagaa     1560

auguguauaa aagauuuccu auuaaauaau uaugacuuac cagccggugc aguguguagg     1620

cucuaucauc cuucuaugau cgacacauac ccagacgcaa aagcaaauaa auacggagua     1680

cuacaauuag guucaccagc cacaaacgca auaaggaacc cuauggcuau gcgaucucua     1740

caugagcuca gacauguugu caauuuacua cucagaaaag ggaaaauuga ucaaaauacu     1800

gaaguacaug uggaguaugc gcgagaacua aaugaugcaa acaaacgaaa agccauugca     1860

gauagacaaa gggagcuaga uaaauuacau aagaaauacc augacgacau aauaacauua     1920

uacaaagaag aaacaggaaa agauauaaac ccaacacaag cagauauuuu aaaauuccag     1980

cuaugggaag aacaagaaca uguaugucua uauacaggaa ggcaaauagg aauaaguaaa     2040

uuccucggac ccgacccaga guucgauauc gaacauacag ugccaagauc aguaggcggu     2100

gacucaaccc aaaugaacau gacccucugc gacagacguu uuaauaggga uguaaagaaa     2160

gcuaugcuac caucucaacu gccaaauuau caagaaauac uaccacgaau ugaaaaaugg     2220

aagaagaaau acgaaaaacu aacaaaagau auagaccauu gcauuaccca cucaggaaug     2280

acaaaagaag ccaaagacuc aauuauccag aaaagacacc ggcuaacacu agaacacgac     2340

uauuggcgga auaaauacuc gagauuuaca augacagaag ucccugaagg cuucucucga     2400

cgccaaggag caggaauagg auuagucuca aaauacgcag gccuauuccu aaaaagucug     2460

uuccaugauc ccacagaucg acgacgauca cauguauaug ugguaaaagg aacaacaacu     2520

gcagaauuua ggaaaaugug gggauuacaa agcgaauaug aaaagaaaag ucgagauaac     2580

cauguacauc acugcguaga ugcaaucacu aucgcgugua uagggaaaaa cgaguacaac     2640

aagauggcuu cauuuuauca ugacgaagaa gcguuugaaa gaggagagug ugaaaaacca     2700

cauuucgaua aaccauggcc aacauucaca caagaugucu uaggacuaga gaaaagacuu     2760

aucguaguac acagcacgca agacaacaug cccaaacaug cgaagaaaua uguaagaacu     2820

ccuagaggaa aauucauugc aaaaggggau ugugcuagag gcucauuaca ucucgacaca     2880

uacuauggag caaucaaaca uaaagaugau acagaaccca gauacguauu aagaagagaa     2940

cuuucuaguu uugacagcgu aaaugaucua gauauaauag uugacgacgc aguccgagag     3000

aaaauuaaag aagcaguaaa agguaaaagu ggcaaagccu uuaaagaggc uuuagcuaau     3060

guuuauauga acaaagaaaa agauaucaga auaaagaaag uuagauguua cguucccucg     3120

guaaaaagac cgauugacau cagacaucaa agagauaugu cccgcuuuga cuacaaaaga     3180

caauuccacg uagcuaacga cgguaacuau augauggcua uauaugaagg ggucguaaaa     3240

ggaaaauuaa aacgagacuu ugaacucgug aaaaauauag aagcagcaag uuacuucaaa     3300

guaagucaag acaacgaaag uuuuuccucu auuguacccg aaaaaucauc aaauggcuuc     3360

cccuuaaaag caguucugaa aauaggaaca cacguauuau uauaugaaaa uacagcaucc     3420

gaaauagacu ucucagaucc aguugacuua acaaggagac ucuauaaagu cguaggauua     3480

agcucagcaa uaauaaauaa uugcucauau ggauuuauua cuuuaagauu ccaucaagaa     3540

gcccgacaag ccaaaaauuu aaaacugacc aaaggaguau ucaaaaacgu cgauaauuac     3600

agaggagcua uuauccuaua ucacacacaa cugaaagcac uuguagaagg auacgacuuu     3660

gauauaaaca uuuuggguga aauaacaucg aaacaguga                            3699


<210>  5218
<211>  3705
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5218
augcccaacu ucuggucgau auucaccgaa aauuuacaaa cauaucuaau gaaaacgcgg       60

auucuaggcc uggacaccgg uacuaauucu cuuggauggg cagucguaga ccgugacgaa      120

aacaaccaau acacaccaau augucgaggu gauuuaauau ucacggaagg cguaaaacaa      180

gaaaaaggua ucgaguucag ccuagcagca gaaaggacau cguauagagc uucaagacgu      240

cacuacuuca gacgaaggcu aagaaaagua gaaguauuaa aaguuuuagu caaacauaaa      300

cuauguccac cccuuucaga ugaggcuuua aacagauggc auaccaagaa agaauacccc      360

cuagaugaag auuuuauucu auggcaaaga accaaugaag cagauaacaa aaaccccuac      420

cacgaccgcc auauaugccu acauagaaaa cugaacuuaa aagauuccca aaccgaucgg      480

uacacauuag gccgugcacu auaccacuua acacagcgga gaggcuucuu aucaaacaga      540

cuagaucaaa augaagaagc cgaagaaaca gguaaaguaa aauccgcaau aucggaacua      600

agcaaagaaa ugaaagaagc cggaugcgaa uuucuaggag auuauuuuua uuuauuauau      660

aggacagaag gaaacagagu acgcaucaga ucuagauaua ccgacagaga aaaacauuac      720

cuacaagaau uuaaagaaau augcaggguc caagaauuag augaagaccu agcaaaggcc      780

cuagagaaaa cgauauucuu ccaacgccca cuuaaaucac aacgacaagg aguaggaaaa      840

ugcacuaugg aaaaaggaca uucgaggugu gcagugucac acccacugua cgaagaauau      900

aggcuucucu gcuacuuaaa uaauauacga auuaaaacac ccucagauga cagauuaaga      960

ccccuaaaug cagaagaaag agagaaaaua acaccgcuau ucuauaaaga gaaaaauuuu     1020

gauuuugaga aaauagcuaa gaaacucgca ggaaaaacac cuuucgguug gaucagagac     1080

gccgaagauu ggccauauca auuuaacuac cgaaugaccc aagaagcacc agcgugccca     1140

acaacagcuc aacuaauaaa aguauucggg gaagacugga aagcugcucu agcugaaacg     1200

uacacaaugu ccgcuaaaca ggaugguacc cuuaaaacag aagacgaaau cugcacugac     1260

aucuggaacg uauuauacag cuuuucauca aaagaaaaac uuaaagaguu cggguuaaag     1320

aaauuacaac uagaugaagu ucaggcaaag gcuuuuucug aaauaagacu gacucgagac     1380

uucgcaagcc ugucauuaaa agccauaagg aaaauacugu ucuuucuccg aaccggcgaa     1440

gacuauucca aagcgauauu uuuagcaaau auucccacaa uagucggaga agaaauaugg     1500

aaugacauag aaagaagguc aagacuccuc uacaaaguag aucucgcacu ucgaccucag     1560

gaagaacgag augcugagga gcacaggacc auagcagaac ggauuaaagc cgcuguagca     1620

gcagaagucc ccgacgcccc cauagacuug uuauaccauc ccuccaugau agaggcauau     1680

ccagaugcca agcgaaacga aagguuugau uguuaccaau uaggaagucc aaaaacaaau     1740

gcagugagaa auccaauggc uaugcgaagu uuacauaugg ucagaaaagu aauaaaucau     1800

uuacuaagaa aacauauaau agaugagaaa acagaaaucc acaucgaaua ugcgagagaa     1860

uuaaaugacg ccaacaaaag acaagcaauc gcagauuggc aacgagaauu aagcaaaaga     1920

cacacagcau augcucaaaa uauaagacaa cuauauaaag cugaaacugg gcuaguaauu     1980

gagccaacaa aggcugauau auuaaaauau caacuuuggg aagaacagca gcacgucugu     2040

cuguauacag gaaagaaaau cggaauagcc gacuuuuugg gagccaaucc aaaauuugac     2100

auagaacaca caauaccgcg uucuagagga ggcgacucua cucaagaaaa cuuaacauua     2160

ugugaaucaa aauuuaaucg agacauaaaa cgggauaaau uaccaacaga gcuugccaac     2220

uaugaagaga uacucgccag aauagaacac uggaagaaac aaugcuaugc acucaaauua     2280

aaaagagaug gcauacgaac acacagugga cuugagaaaa gcauaaaaga caguagaaua     2340

cgacguagac accuuguagg auuagaauau ggauaccuaa aagguaaaua cgauagauuu     2400

accaugaaag aaguaccaga aggauuuuca cgacgacaag gagccgggau uggccuaauc     2460

ucuaaauacg caggucucuu ccuaaaaagc cuauucaagg auccuaaaaa cccaaaccga     2520

agcaaugucu acguaguaaa aggugccaua acagcagagu ucagaaaacu auggggucuc     2580

caacaagcag acgaagcaaa aagccgagaa aaucacacac accacuguau cgaugcuuac     2640

acaauagcau guauagggcc cugugaauau gccgcucucg cagccuauua uaggagugac     2700

gaagaguuca aauacggaag aagacgugaa aaaccacaau uugaaaaacc uuggccaacu     2760

uucaccgaag accuccuaaa auuacaagaa gaauuacuaa uaguacauca aacaaccgau     2820

aaauuaggga agagagaucg aagaaaagua aaaacgcccc gaggaaaauu ccuaacagga     2880

ggugacagcg caaggggucg ccuacaucaa gaaacauacu auggugcgau aaacuacgac     2940

ggaaacauua aauacguagu aagaaaaccg cuagauuccu uaacagaaaa agaccuagau     3000

aaaauaguag acgaaacagu aaugggaauu guuaaaggug caguagaagc uaaaggauca     3060

cuuaaagaag cacucgcuga cggcauauau augaauaaag aaaaaggaau auuaauaaag     3120

agaguaagaa uuuucucaga cgucucggaa ccaauaaacc uaaagcaauu gcgagauguu     3180

agcacaaaag aauauaaacg acauauacau cugagaaaag augaaaacua ccuguuugca     3240

aucuaugaag gaauagacaa gaagaaaaau aagaaacaaa cagaaaccgc cacaguggau     3300

uaccuagacg cagcaaaauu uuauaaagau agcaaacgua gagacaaagc gaauaguuua     3360

guaggagauu acucaaagaa aaauaaauua ccguugaaau guauuuuaaa gaaacgacaa     3420

auggugcucu uuuacgaaca cucucccgaa gaaauuagau ucaaagauaa gaaagauuug     3480

ucgcgaagac uauacauagu aacuagcaua gccaaagacg guagagucaa auuucuccau     3540

caccaagcag cgacagcgac cgguauccaa ucaucgccau caccguuuac aaacgacggc     3600

gaacaucaac cuuauuaucg acuuagugca uccaauauaa aaguacuugu ggcuggaauu     3660

gacuucgaca uaaacauucu aggagaaaua acacccaaau uauga                     3705


<210>  5219
<211>  3711
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5219
auguauuucu acaaaaacaa agaaaauaaa uuaaacaaga aagucguacu gggucuagau       60

cuagguauag caucagucgg auggugcuua acugacauau cacaaaaaga agauaacaaa      120

uuucccauaa uauuacaugg agugagacug uuugaaacag uagacgacuc cgacgacaaa      180

cuuuuaaacg aaacacgaag aaagaaaaga ggccaaagac gccggaacag acgauuauuu      240

acccguaaaa gggacuuuau uaaauaccua auagauaaca acauaauaga acuagaauuu      300

gauaaaaauc caaaaauccu agugagaaac uuuauagaaa aauacauaaa cccauucagc      360

aaaaaccuag aacuaaaaua caaaucagua accaaucuac cuauagguuu ccacaacuua      420

cguaaagcag cgaucaacga aaaauacaaa cuagacaaaa gugaacucau aguacuacua      480

uacuucuacc uaucccuaag aggcgccuuc uuugauaacc cagaagacac caaaucaaaa      540

gaaaugaaca aaaacgaaau agaaauuuuc gacaaaaacg aaucaaucaa aaacgcagag      600

uucccaaucg acaaaaucau cgaauuuuac aaaauaucag gaaaaauuag aucaacaauc      660

aacuuaaaau ucggucacca agacuacuua aaagaaauca aacaagucuu cgaaaaacaa      720

aacauagauu ucaugaacua cgaaaaauuc gcuauggaag aaaaaucauu cuucagccgg      780

auaagaaacu auucagaagg cccgggcaac gaaaaauccu ucuccaaaua cggacucuau      840

gcuaacgaaa acggaaaucc agaauuaauc aucaacgaaa aaggacagaa aauauacacg      900

aaaauuuuca aaacccuuug ggaaucaaaa aucggaaaau gcucauauga uaagaaacuc      960

uaccgagcac cuaaaaacuc cuuuucagcc aaaguauucg acaucacaaa caaauuaaca     1020

gacuggaaac acaaaaauga auauaucagc gaacgccuga aaagaaaaau ucuucuauca     1080

cgauuucuaa acaaagauuc aaaaagugcc gucgagaaaa uacuaaaaga agagaacaua     1140

aaauucgaaa aucuaagcga aauagcauac aacaaagacg auaacaaaau aaaccugcca     1200

aucauaaaug cuuaucauag cuuaaccaca aucuucaaga aacaccucau aaacuucgaa     1260

aauuauuuaa uaucaaacga aaacgaccua ucaaaacuca ugaguuucua caaacaacaa     1320

ucugaaaaau uauucguccc uaaugaaaaa ggcuccuacg agauuaauca aaauaauaau     1380

guuuugcaua uauucgacgc aauuaguaac auccuaaaca aauuuucaac aauacaagac     1440

cgcauuagaa uacuagaagg auacuucgaa uuuucgaacc ucaagaaaga cguaaaaagc     1500

agcgaaauuu acucagaaau cgccaaacuc cgcgaauuua gcggcaccag cucccuuagu     1560

uucggagcau auuauaaauu cauuccgaac cucauaucag aaggcagcaa aaauuacucg     1620

acaauaucau augaagaaaa agcauuacaa aaucagaaaa auaacuuuuc ccauucaaau     1680

cucuucgaaa agacuugggu cgaagaccua aucgccucac ccacagucaa aagaucguua     1740

cggcaaacaa ugaaucuauu gaaagaaaua uuuaaauacu cagagaaaaa caaccucgaa     1800

auagagaaaa uaguuguaga agucacccga ucaucaaaua auaaacauga aaggaagaaa     1860

auagaaggua ucaacaaaua uagaaaagaa aaauaugaag aacucaagaa aguauaugac     1920

cuacccaaug aaaacacaac ccuucuaaag aaauuauggc uacucagaca acaacaaggc     1980

uacgaugcau acucauuaag aaaaaucgaa gcaaaugacg uaauaaauaa accauggaac     2040

uaugacauug aucacauagu uccaagaucc aucagcuucg acgacuccuu cuccaaucua     2100

guaauaguaa acaaacuuga caaugccaag aaaucaaacg accucucagc aaaacaauuc     2160

aucgaaaaga uauauggaau agaaaaacua aaagaagcua aagaaaacug gggaaacugg     2220

uaucuacgca acgcaaacgg aaaagcauuc aaugacaaag guaaauuuau caaauuauau     2280

accaucgaua acuuagacga auuugauaac agcgacuuua uuaaucguaa cuuauccgac     2340

accucauaca uaacaaacgc ccucguaaau caccucaccu ucucaaauuc aaaauacaaa     2400

uacuccgucg uuucgguuaa cggaaaacaa acauccaacu uaagaaacca aaucgccuuu     2460

guaggcauca aaaacaacaa agaaaccgaa agagaaugga aaagaccgga aggauuuaaa     2520

ucuauuaauu ccaaugacuu cuuaauaaga gaagaaggca aaaacgaugu uaaagaugau     2580

gugcuuauca aagaccgaag uuucaaugga caccaugcag aagaugcuua cuuuaucacc     2640

aucauaucac aauauuuucg aaguuucaaa agaauagaaa gauuaaaugu aaauuacaga     2700

aaagaaacua gagaauuaga cgaucuagag aaaaacaaca uuaaauuuaa ggaaaaggcu     2760

uccuuugaca acuuccuacu gaucaacgcg cuugacgaac ucaaugagaa acucaaucaa     2820

augagauucu cuagaauggu cauaacaaag aaaaauaccc aacuauucaa ugaaacauug     2880

uauucaggua aauaugacaa agggaaaaac accaucaaga aagucgaaaa acucaaccua     2940

cucgauaaua gaacagauaa aaucaagaaa auagaagaau uuuucgauga agacaaacua     3000

aaagaaaacg aacuaacaaa acuacacauu uucaaucacg acaaaaaucu auacgaaacu     3060

uuaaaaauaa uauggaauga agugaaaaua gaaaucaaaa auaaaaauuu aaaugaaaag     3120

aauuauuuca aauacuuugu caauaagaaa cuucaagaag gaaagaucuc guuuaacgaa     3180

uggguaccaa uucuugauaa ugacuuuaaa auaauaagaa aaauuagaua cauaaaauuc     3240

agcucugaag aaaaagaaac ggaugaaaua auauucuccc aaaguaacuu cuuaaaaaua     3300

gaccaacguc agaauuucuc auuccacaau acccucuacu ggguacaaau auggguuuac     3360

aaaaaccaaa aagaucaaua cugcuucauc ucuaucgacg caagaaacuc aaaguucgag     3420

aaggaugaaa uaaaaauuaa uuacgaaaaa uuaaagacac aaaaagaaaa auuacaaauc     3480

aucaacgaag aaccgauacu gaagauaaau aaaggcgacc uuuucgaaaa cgaagaaaaa     3540

gaacuauucu acauaguagg acgggacgaa aaaccccaaa aauuagaaau uaaauacaua     3600

cugggcaaga aaauaaagga ccaaaaacaa auacaaaaac ccgucaagaa auauuuccca     3660

aauuggaaga aaguuaacuu aacauauaug ggagaaauuu uuaagaaaug a              3711


<210>  5220
<211>  3717
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5220
augacgaaag uauuaggacu ugaccuaggc gcugcuucug ucggaugggc caugauagaa       60

gaagaggaaa aaucacacaa aauacuaggu cucgguucaa gaauaauucc guaugaagga      120

acagaaggaa acgacuucgc caaagggacu ggcgagagcc gaaaugcacu uagaacaaaa      180

gcacgcaccg cuagaaaagg auacgauaga uaccaauuaa ggagaaaaua uuuaguggau      240

acguuaguca aaaacgaaau gaugccagau gaauacuuaa gaaaauuacc caaaaugcag      300

uuaugggaac uaagaucuaa agcuguuaca accgaaauaa caaaacaaga gcucggaaga      360

cuauuucuau ggcuaaauca gaaaagaggu uacaagucaa gccgaucuga cgcaaaccuc      420

gacaagaaag acacagaaua cgucgucgaa gucaaaucaa ggcaugaaaa aauaaaagaa      480

ucuggcuuua caaucgguca auauuuuuau ucagaauuga aaaguaauga auacuucaga      540

guaaaagaaa auaucuuucc ucgagaagca uacauagaag aauucgacgc cauaugcaaa      600

caacaaaaac gacaccuuac cgacaaauua auaaguagaa uacgaaauga aaucaucuau      660

uaccaaagac cccuuaagag ucaaaaagga uugguauccg uaugucgauu ugaagguuuc      720

uuuaaaaaag gaauaaaugg aaaagaauac uucguuggac caaaggucgc accaaaauca      780

agcccacuau uucagaccgu aaaaauaugg gaaaauauua acucuauccg ucucucugac      840

cgaaauggga aggagauuau gcuaucacua gaacaaaaga aauguaucuu caaccaucua      900

gauaacaaug aaaaacuaac accaaaagac uuauuuauaa ucauaggagu uaaaaaagag      960

aauugcuucg uaaacaaaca acuagaaaaa ggaauucaag gcaauguuac uaaaagcacg     1020

auacuaaagu guuuuggaga aaauaacgac uaugauaaau gguuaaaauu agaccucaac     1080

auuaucguaa aaaaagacac uuuuggcuau cuuuaugaca aaaaaacugg ggaaauuaua     1140

ggcgaaaaac uaauaaaaca cguagacucg gcaguagaaa gagaaccauu auaucaacua     1200

uggcacacaa ucuacucaau aaacaauacc gacgaauguu ccgcugcgcu acaaaacaaa     1260

uucggcuucg acucagaaac ggccgacaaa uuaucaaaca ucgauuucgg caaagcagga     1320

uucggaaaua aaucagcuaa gguaauaaga aaaauacuuc cauaccuuau ggaaggugac     1380

gacuacucac aaucaaugag cuaugcaggu uucaaccacu cagauucacu aacuaaagau     1440

gaaaacuuaa aacgaaaauu auuagauaaa cuaagaccaa ucgaaaaaaa uucccuacgu     1500

caaccuauag uggaaaaaau uuuaaaucaa auggugaaug ugguaaacgc aauuauagau     1560

aaauacggaa aaccagacga aauacgaauc gaacuagcaa gagaacuaaa acaaaacaaa     1620

gaagaaagga acaaaacaga caaagauaug ucaaaaagac aacgagaaaa uggagaaaua     1680

ucaaagaggu uagcagaaua uggccuucgc gccacacgua acaacaucau caaauggaga     1740

uuauaugaag aaauagacaa ucaagauaaa aaauuaaacg cuauuugugu cuauugugga     1800

caaccuauaa guuuaacaga agcaauuaaa ggcaacgaag uugacgugga acauauaaua     1860

ccuaaaucaa aacuauuuga cgauagccaa agcaacaaaa cacuugcaca ccggcgaugu     1920

aauaguacaa aaggagacau gacagcauac gauuuuaugg aaaauaaacc cgcacaagua     1980

uuuaacgcuu acauugaaag gguaaacaug cuauacgaca auaaaauaau uagaaaaguc     2040

aagcgagaca agcuacuuau gccagagucc aaaauuccug auaacuucau agauagacaa     2100

cuuagagaaa gccaauauau cgccucaaaa gccagagaaa uauuacaaac aaucuguuau     2160

aauguaugga gcacaucagg aacagugacc gcccgucuga gacacuuaug gggcugggac     2220

gacgcaacaa ugucacucca auuaccaaaa uaucgggaau uagguuuaac agaaauggua     2280

gaaugggaaa gugaacaugg caaacagaaa cauagcaaag aagucaucuc cgacuggucu     2340

aaaagagaug aucacaggca ccaugcagua gacgcacuaa cgauagcaug uaccaaacaa     2400

ggcuuuauac aacgauucaa cacgcuuaac aguucuaaaa cgaaagaaga cauggaguuu     2460

gagaucgcaa aguguucaau agaauauaga gaaaaccuau caaaauuaga aaaauacauu     2520

aucgcauuaa gaccuauucc gguuucggaa guugaagacg caguugcuaa cauacuaaua     2580

aguuuuaaau ccggcaaaaa aguugccguu aaaggaaaaa gaaaaauagg aaaacgaggu     2640

aauaagaaaa uaauacaaac cgacaucaua auuccacacg guucacuauc ggaggagagu     2700

guguauggaa aaauaaaaac aauagaacgu gaaaaaccgc uaaaauaccu auuccaaaau     2760

agucaacuaa uauucaacaa uaaaauuaaa auuuuaguag aagaaagacu agcaacauuu     2820

gaaaacgaca caaaaaaagc gcuagcaucc uuaaaaaaag uaccaguuua ucuagacgaa     2880

gaaaaaacaa uuguacucga auacgcgacc uguuuuaaag aggaauacgu aaaaaaauau     2940

gacguagaua caaacuuuag uaaaacagau aagguuauag acgacaaaau caaagaaauu     3000

cuacaacacc gacuaggaaa auuugguggu aaagcaaaag aaaaagaggc auuuaaagac     3060

guucaacaag gugauaaaac aauaaaaugg uacgaggacg aaggacuaga aagaccaaua     3120

cauucuguac ggugcuucac aggccucucu gccauuguac caguaaaaaa agacgaaaac     3180

ggcaucgaua ucggguucgu aaagccaggu aacaaucauc acauugcaau auauucagac     3240

aaagacggga accgcuauga acauauaugu acauucuggc acgcagucga aagaaaaaaa     3300

uacgacauuc caguaguuau uaaagacuca aacgaaauau ggaacaaaau cuggucaaaa     3360

ccagaaggau cuuacccgca aaguuuucua gacaaauuac cacaggaaaa ccucacucug     3420

gaccuuucaa ugcaacaaaa cgaaauguua auauuaggga ugagcuauga agacauuaaa     3480

acggcuaucg acaacucaga cuauaaacaa uuaagcucaa aucuauacag auuacaaaaa     3540

cuugcaaacg caaacuauau guuccgacau caccuugaga cacaaaucau ugaugauaau     3600

aauucaaaag caagcaggag auuuauaaac auucacucau uaaaagcauu auucgcuugg     3660

aaccccuaca aaguuaacau aaacugucua ggagacauac aaacaaacuc uagauga        3717


<210>  5221
<211>  3720
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5221
augacagaaa aacgaaaauu ugauauuagg gaagucuacc ucgcaauaca ucauaucgua       60

aaguauagag gaaacuuccu aaauagcgca acaguugaua gcuucaaaac auccaauaua      120

gacuuuacau cacaauucga cagauuaaac gaacuauaca gacaagucau uuuagaagaa      180

ccuuuucaaa uaaacaugaa ucaagucgac aaaaugacua acaaauuacu agauaaugac      240

gcguuaaaac ucgauacaca aaaacaagua gccaaacucc uaccuguaau auacaaugac      300

aaacccgucg acaaacaaua cacaagguua gcuacagagu uuuccaaagc cauacuugga      360

uacaaaacaa aacuagaugu uauuuuaaac uuagacaccc aaaaugcaaa agauugggca      420

auccgauuag augaugaaga caucgaugac aaguuaccag cccuaguaga aaauuuagac      480

gaaucuagac aagagauagu cacaauaauu agagaccuau acgcacaaau aacccugaau      540

gcuauuguuc caaaaggcaa aagcuuauca gagucaaugg uagacaagua uaacgaccac      600

aaagaucacc uagauuuauu aauggggcuc auucacgaac uaggagauga uuccgcaaaa      660

ggaaucaaac uaagagaagc auauacacaa uacguaggaa aaagcggaga uaaaacauua      720

aaccaagaug auuucuacgc cgccauuaaa aaaaaccuug augaagacag uaaaagauca      780

ccaaaaauac aacgauuaau ugaacaagca aguuuuaugc cuaaacaacg aaccucagcu      840

aauggcguua uaccacacca acugcaucaa uuagaacucg acagaauaau ugaaaaccaa      900

ggccgauacu auccauuucu aaaagaaccu aacaguaaua cacauaaacc aaacggaggu      960

aaauauaagc ucgacgaacu agucgcauuc aggauuccau auuacgucgg cccacuaaua     1020

acaaagcaag aucaagaaaa aacaucugga gcugauuucg ccuggaugau aagaaaaccg     1080

ggacaaggag gagagauuac accuuggaau uucgaucaag aaguagacag aauggccuca     1140

gcaaacacuu uuauaagaag aaugacgacu aaagauucau aucuaguagc ugaagacgua     1200

cuaccagauu caucgcucau auaugaaaaa uucaaaguac uaaacgaacu caauauguua     1260

aaagucaaug accgaagauu gucaauauca caaaaacaag accuauucaa uaaucuuuuc     1320

aaaaaacaaa agacaauaag aacuaaaaaa uuacaaacau auauaagaac uaaaugggaa     1380

cuaccauccg uaaugauauc cggucuaucg gaucccgaca aauucaauuc uuccuuagca     1440

accuacaucg acuuucgaga cgucuuugga gaaaaaguug acgaccccaa ucgucaagca     1500

gacuacgaaa aaauaauaga auggucgacc guauucgaag acagaaaaau auaccaagcc     1560

aagcuaaacc aacuagacug guuaaccgaa agccaaagca aggcauuauu aacgaaacau     1620

uauaccggcu ggggaaggcu uagcaaaaaa cuauuaaccg gacuuaaaga ucaaaacgga     1680

gauaguauac uagaucaacu auggaaaacc aacgacaacu ucaugcaaau acaaucuaga     1740

gaugaauuug caaaacaaau ucaugaugag aaugccaaac aguuucacga agccaauaac     1800

guagaugaca ucuuagacga cgccuuuacu ucaccacaaa auaaaaaagc aauuagacaa     1860

guagauaaag ucguacagga cguaguaaag gcaguaggau augcacccga uaaaauagcc     1920

auagaguuua cccguagucc acaagauaga ccacaaagaa cauucaguag acaacgacaa     1980

uuacaaagca ccuaccuaaa cgucgcaaaa gcauuaauga aaucaaaucu aaaccaagaa     2040

cucaagucag uaauagauuc acagcagacu cuaacugaua aacuauaccu auacuuuaca     2100

caacucgguc aagacaugua uacuggaaaa gagauaaauu ucgaugaauu aguaaauuac     2160

caaauagauc acauuuuacc ucaagcauuc auaaaagaug auucuuuaga uaauagagua     2220

uuaguuucag cccccauaaa uaaugcaaaa agugauaaug uaccaguuaa aaaauuugga     2280

gccaaaaugg guuacuucug gaaacaacua gcagagaacc aucuuaucuc aaaaagaaaa     2340

cucaauaauu uaaccacaga uccagacaag auaggaaaau ucaccgcuca aggauuuaua     2400

caccgacaac uaguggaaac gucacaagua auaagacuag uugcaaauau ucucggaaau     2460

gaauauggac augacggaac gacaauaauc gaaguuacag caaaaaugaa ccaucaaaug     2520

cgaaaagacu uugacuugau aaaaauaagg gaugucaaug acuaucauca cgccauggau     2580

gcuuauuuaa cagccuacgu cggcgauuac cucuaccuaa gauauccgaa auuaagaagc     2640

uauuuuguau auggagacuu uaaaaaacua aaagaucaaa gucuaaaaau cagaaauuuu     2700

aacuuuuuac acgauuuaac uaacgaugaa gccggagaua aaauaguaga ccaagaaaca     2760

ggugaaauua uaugggacaa acaugaaucc guaaaacaau uaaaaaaagu auaucacuac     2820

aaguucaugu uagugucuca agaaguauau acccgacaag acgcccuauu caaucaaaca     2880

aucuaucccg cuucagacgc ugauaaaaga aaacucaucc cgaucaagaa caacaaacca     2940

gucgauguau acggaggcua uucagguaau gucgacgcuu acauggccau cgucagaaua     3000

cauggaaaga aagaagauaa auacaaagua guaggaguac caaugcgcgc uguugccgau     3060

uuaaaaaaag ccgaaucaaa aggacgagau aacuaucuga augcaguaca ugaagucuua     3120

aaaccacagu uuacaaaaaa aaaaaaagau cgaaaaacag gugaaauuac ugaaaugaug     3180

gacgacuucg acguuuuauu agguaaagua uacuauagac aacugauugu agauggaaac     3240

aaaaaauuua ugcuaggauc cucaacauac caauauaacg caaaacaauu aguucuauca     3300

gacaaagcca ugcaaguccu aaguaaagac gauaaauuaa agaaccaaga cgaaaaucaa     3360

aauuuaauag acguuuauga ugaaauacua gaaaaaguag accaauauuu ugaauuguac     3420

gauauaaaca aguucaggca aaaacuacac gacggccgaa aaaaauuuau agaacuucca     3480

guagacaaug auuuuaacgg aaagaaauua aucucauaug gaaaacgagc caccauuauu     3540

ucaauacuaa auggucuaca cgcaaacgcg accaugucaa acuugaagua ccucggaauu     3600

ucaucgccuu ucggaaugcu ccaaguaccu aacgguauaa uacuuucucc agccucacga     3660

aucugcuacc aauccccaac aggacuauuc gagagaaaag uaaaacuauc agaucuauga     3720


<210>  5222
<211>  3738
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5222
augauaauuu acuucauuuu cgagaaaauu guaaauacua uucuaguaau gaagaaaaua       60

gugggacuag accuaggaac caauucaaua ggcugggcau uaauaaaugc cuacauagau      120

gaauauaaca aagaacaccu auacgguaua gaagcaugcg gaagcagaau uauaccaaug      180

gacgccgcaa uacuaggaaa cuuugacaaa ggaaacucaa uaucacaaac cgcagaccga      240

acuucguaca ggggcauccg aagacuacga gaaagacauu uauuaagaag agaacgacua      300

cauagaauac uagacuuacu uggcuuucuc ccaaaacacu acucugacuc ccuuaacagg      360

uaugguaaau uccuaaacga caucgaaugc aaacuccccu ggguaaaaga cgaaacggga      420

aguuacaaau uuaucuuuca agaaucuuuc aaagaaaugc uagcagacuu caccgaacau      480

cauccuaaau uaaucgcaaa caacaagaaa guuccauaug auuggacaau auacuaccuu      540

agaaagaaag cacuaacuca gaaaauauca aaagaagaac uugcauggau acuuuuaaac      600

uucaaccaga aaaggggcua uuaucaauua agaggcgaag aagaagaaac gccgaauaaa      660

uugguugaau auuacucucu aaaagucgaa aaagucgaag auaguggaga aagaaaagga      720

aaagacacau gguauaacgu ccauuuagaa aauggaauga uuuaucggcg aaccagcaau      780

auuccgcucg auugggaagg gaaaacaaaa gaguucaucg uuacaacaga ccuagaagca      840

gacggcaguc cuaagaaaga uaaagaagga aauaucaaaa gaucuuuuag ggcaccaaaa      900

gaugacgacu ggaccuuaau caaaaagaaa acagaagccg acauagacaa aauuaaaaug      960

acuguaggcg cguacauuua ugacacauug cuacaaaaac cugaccagaa aauaagaggc     1020

aaauuagucc gaaccauaga aagaaaauac uacaaaaaug aacuauauca aauacuaaaa     1080

acacaaucag aguuccauga agaacucaga gauaaacaau uauacauugc auguuuaaac     1140

gaacucuacc ccaacaauga accacgccgc aacaguaucu caacgcgaga uuucugucau     1200

uuauucauug aagacauaau cuuuuaccaa agaccauuaa aaagcaagaa aagccuaaua     1260

gacaacuguc cauacgaaga aaacagauau auagauaaag aaucaggcga aauaaaacau     1320

gccucaauca aaugcauagc gaaaagccac ccacuauauc aagaauuuag auuauggcaa     1380

uucauaguga aucucagaau cuacagaaaa gaaacagaug ucgacgucac acaagaacuc     1440

uugccaacgg aagcagauua uguaacccuc uuugaauggc uaaaugagaa gaaagaaaua     1500

gaccaaaaag cauucuuuaa auacccacca uuuggauuca agaaaaccac aucaaauuac     1560

cgcuggaauu acgucgaaga caaaccauau ccaugcaaug aaacccaugc acaaauuaua     1620

acaagacuag gaaaagcuca caucccaaaa gcauuucucu caaaagaaaa ggaagaaaca     1680

cuauggcaua uccuauacag cauugaagac aaacaagaaa uugaaaaagc auuacauucu     1740

uuugcuaaua aaaacaaccu aucagaagag uucauagaac aauuuaaaaa cuucccacca     1800

uucaagaaag aauacggcuc uuauucagcu aaagccauaa agaaauuauu gcccuuaaug     1860

cgaaugggca aauauuggag uauugaaaac auagacaaug gcacacgaau uagaauaaac     1920

aaaauuaucg acggcgaaua ugacgaaaau auaagagagc gaguccgcca aaaagcaaua     1980

aaccucaccg auauaaccca cuucagagcu cuaccacuau ggcuagcuug uuaucuggug     2040

uaugacagac auucagaagu aaaagauauc gugaaaugga aaacaccuaa agauaucgau     2100

uuauaccuaa aaaguuuuaa acaacacuca cuucgcaacc caauaguaga acaagucaua     2160

acagaaacau uaagaacggu ccgagacaua uggcaacaag ugggacauau cgacgaaaua     2220

cacauagaac ucggccguga aaugaaaaac ccagcugaca aacgagcuag aaugagucaa     2280

caaaugauca aaaacgaaaa uacaaaucug cgaauaaaag cucuacucac cgaguuccuu     2340

aacccagagu ucggcauaga aaacguacga ccauacuccc caagccaaca agaucuacuu     2400

cguauauacg aggaaggagu acuuaacuca auuuuagaac ucccagagga uaucgguaua     2460

aucuuaggca aauucaacca aacagauacu uugaaacguc caacucgcuc ugaaauucuc     2520

agauacaaac uguggcucga acaaaaauau cguagucccu acacaggaga aaugauaccc     2580

cuaucuaaac uauuuacccc agcguacgaa auagaacaua uuauaccaca aaguagauau     2640

uucgaugacu cacuaucaaa caaagucauu ugugaaucag aaauaaacaa acuaaaagau     2700

agaucacucg gauacgaguu caucaaaaac caucacggag aaaaagucga auuagccuuc     2760

gauaaacccg uagaaguauu aucaguugaa gcauacgaaa aacuuguaca ugaauccuau     2820

ucacauaacc guuccaaaau gaagaaacua uuaauggaag auauacccga ccaauucaua     2880

gaacggcaac uuaaugacuc aagauauaua agcaaagucg ucaaauccuu gcucaguaac     2940

auagucagag aagaaaauga acaagaggca aucuccaaaa auguaauacc cuguaccggg     3000

ggaauaacag auagacugaa aaaggacugg ggaaucaacg acguuuggaa uaaaauaguu     3060

cuccccagau uuauaagguu aaaugaauua acugaaucaa cucgauucac aagcaucaac     3120

accaauaaua ccaugauccc aagcaugccu cuugaacuac aaaaaggauu caacaagaaa     3180

agaauagauc auagacauca cgcaauggac gcaauaauaa uugccugcgc aaaccgaaau     3240

aucguaaacu aucuaaacaa cguauccgcc uccaaaaaca cuaaaauuac aagaagagau     3300

cuccaaacuc uuuuauguca uaaagauaaa acagacaaca auggcaacua caaauggguc     3360

auagauaaac caugggagac cuuuacacaa gacacacuaa cugcacuaca gaaaauaaca     3420

guuaguuuua aacaaaaucu acgagucaua aauaaaacaa ccaaccacua ucaacacuau     3480

gaaaacggaa agaaaauugu aucaaaccaa ucaaaaggag auucuugggc aauccgaaaa     3540

agcaugcaca aagagaccgu acauggagaa guuaaucuaa gaaugauaaa aacagucucu     3600

uuaaaugaag cccucaagaa accucaagcc auaguugaaa uggaccucaa aaagaaaaua     3660

cuagcuaugu uagaacucgg auacgauacc aaaagaauua aaaauuauuu ugaagaaaau     3720

aaagacaccu ggcaauga                                                   3738


<210>  5223
<211>  3741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5223
augcgccuag uauauuuggc gcucgcccac augauaaagu uuagaggaca cuuucuauau       60

gaaggagacu uaaaagcaga aaauacagac guacaggcuc uauucaagga uuucguggag      120

gaauacgaua aaacgaucga agaaucacau uuaucagaaa ucaccguaga cgcacuaucc      180

aucuuaaccg agaaaguaag caaguccuca agguuagaaa aucuaauagc ucauuauccg      240

acagaaaaaa aaaacacauu auucggaaau uuaauagcau uaucacucgg uuuacaagcc      300

aauuuuaaaa cuaacuucca auuaagcgaa gacgcaaaac uacaauucag caaagacaca      360

uacgaagagg acuuagaagg ucuucugggu gaagugggcg augaauacgc agauuuauuu      420

gucuccgcga agaaucucua cgaugcaaua uuauuaagcg gaauacugac cguagacgac      480

aauaguacaa aagcuccauu aucagcauca augguaaaaa gauacaaaga acauaaagag      540

gaacuagcag cuuuuaagcg auucauaaaa gaaaaauuac caaaaaaaua cgaagaaaua      600

uuuaaagacg acacaaaaaa cggauacgca ggcuacguag gcgcagacaa aaaacuucga      660

aagagaucag gaaaacucgc aacggaagaa gaauucuaca aauauguaaa aggaauacua      720

aauaaagugg agggcgcuga uuauuuucua gauaaaauag acagagaaga uuuccucagg      780

aaacaacgaa cauuugacaa ugggaguaua ccccaucaaa uccaucucca agaaaugcac      840

gcgauucucc gcaggcaagg cgaacacuac ccauuuuuaa aagaaaacca agauaaaauu      900

gaaaagauac uaacauuccg aauuccauau uacguuggac cguuagccag aaaaggcucg      960

agauuugcau gggccgaaua caaagcagac gagaaaauaa cacccuggaa cuucgaugac     1020

aucuuagaua aagagaaguc agcagaaaaa uucauaacga gaaugacauu aaacgaccuu     1080

uaccuuccag aagaaaaagu acuuccaaaa cacaguccac uauacgaaac auucacagua     1140

uacaaugaac uaacaaaagu uaaauauguu aacgaacaag gggaggcaaa auuuuucgau     1200

acaaauauga aacaagaaau auuugaucau guauuuaaag aaaauagaaa aguuacaaag     1260

gacaaauuac uuaacuacuu aaacaaagaa uuugaagaau ucagaaucgu aaacuuaaca     1320

ggauuagaca aagaaaauaa agcauucaac gcgucucuug gaacuuacca ugacuuaaga     1380

aaaauccucg acaaaucauu ccuagaugac aaagucaacg aaaaaauaau agaagauaua     1440

auccaaaccc uaacacuauu cgaagauaga gaaaugauuc gccaacgccu acaaaaauac     1500

ucugacaucu ucaccacaca acaacugaaa gaacuagaga ggagacauua cacaggaugg     1560

gguagauugu ccuauaaauu gauaaacgga auaagaaaua aagaaaauaa aaaaaccauc     1620

uuagacuauu uaaucgauga cggauacgca aacagaaauu uuaugcaguu aaucaacgac     1680

gaugccuuau cauuuaaaga agaaaucgca cgcgcacaaa uaauagguga cguugacgac     1740

auagccaaug ucguacauga ucuacccggc ucaccagcca uaaagaaagg gauacuccag     1800

uccgucaaaa uagucgacga acuaguuaaa guuaugggac acaacccaac aaacauuaua     1860

auagaaaugg cucgcgaaaa ucaaacuacu gauaaaggac gaagaaacuc acaacaaaga     1920

cuaaaauuac uccaagauuc auuaaaaaau cuagacaauc caguaaacau caaaaacguu     1980

gaaaaccaac aauuacagaa ugacagauua uucuuauauu acauccaaaa ugggaaagac     2040

auguacacag gagaaacacu agauauaaau aauuuaucac aauaugacau agaccauaua     2100

auaccccaag cuuucauaaa agacaauucu cuagauaaua gagucuuaac aagaucagac     2160

aaaaaccgcg gaaaauccga cgauguacca ucaaucgaag uuguacauga aaugaagagu     2220

uucuggagca aauuacucuc cguaaaauua aucacccaaa gaaaauucga caaucuaaca     2280

aaagcugaaa gaggaggacu uacagaaaau gauaaagcgg gcuuuaucaa acggcaauua     2340

guugaaacga gacaaauuac aaaacacguc gcacaagucc uagaugcaag auucaaugcg     2400

aaacacgacg aaaacaaaaa aguaauacga gaugucaaaa uaaucacauu aaaaucaaac     2460

cuaguaaguc aauucaggaa agauuucaag uucuacaaag uacgugaaau caacgacuac     2520

caccaugcac augaugccua ucuuaacgcg gucaucggca cugcgcuccu aaaaaaguac     2580

ccgaaacuug caagugaauu cguguaugga gaauucaaaa aauacgacgu aagaaaguuu     2640

aucgcaaaau cagacaaaga aaucgggaaa gcaacagcaa aauacuucuu uuauuccaau     2700

cuuaugaacu uuuucaaaaa agagguaaaa uuugccgaug gaacaguugu ggaaaggccc     2760

gacauagaaa caucugaaga cggagaaaua gcauggaaca aacaaacaga uuuuaaaauc     2820

guaagaaaag uauuaucaua uccccaagua aauauaguca aaaaaacaga aguccaaacu     2880

cacggcuuag auaggggaaa gccaaggggg cucuuuaacg cgaaccccag uccgaaaccu     2940

aaaccugaua gcucugaaaa cuuaguagga auaaaacgua acuuagaucc aaaaaaauac     3000

ggagguuaug caggcauuuc aaacucauau gcgguauugg ucaaagcuau aaucgaaaaa     3060

ggcguaaaaa aaaaagaaac caugguuuua gaauuccaag gcaucucaau cuuagacaga     3120

auaacauuug aaaaagauaa acgagcauuu uuacuuggaa aaggcuauaa agacauaaag     3180

aaaaucauag aacugccaaa auauagccua uucgaacuaa aagacggcag cagacggaug     3240

cuagcuucaa uccuuaguac aaauaauaag agaggagaaa uacacaaagg aaacgaacua     3300

uuuguuccuc aaaaauucac cacgcuacua uaccaugcaa aacgcauaaa uaacccuaua     3360

aacaaagacc acauagaaua cguaaaaaag cauagagacg auuuuaaaga auugcucaac     3420

uacguuuuag aauucaacga gaaguacguc ggcgcaacaa aaaacgguga aagacuaaag     3480

gaagcugucg cggacuucga uucaaaaagu aacgaagaaa uaugcaccuc auuuuuaggc     3540

gcagucaaua gcaaaaaugc aggacuauuc gaacuuacau cauuaggauc cgcuucagac     3600

uucgaauucc ucggaguaaa aauaccgcgu uaucgagacu acaccccaag uucacugcua     3660

aaagauagca uacuaauuca ucaaucaauc acaggauuau augagacaag aauagaccuc     3720

ucaaaacuag gagaagacug a                                               3741


<210>  5224
<211>  3747
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5224
augaagaaaa uccuaggccu agaccuaggu agugccagua uuggaugggc agugauaaca       60

gagaaugugg aaaacggcau aacaaaaugc gacaucuuag gcaugggcag ucgaaucauc      120

ccauaugagg gaacugaagg aaaagauuuu accaaaggua caggcgaauc ucguaauagc      180

cuucggacaa aagccaaaac cgcaagaaaa ggcuaugauc gauaccaacu aagaagaaaa      240

uauuuaguag auacacuaau aaaaaaccaa auguacccag acgaagcaac ccgcaaccuc      300

ccaaaaauga aauuauggga auuaagagau aaggccgucc acucuccagu cucguuaaug      360

gaauuaggaa gauuacuacu cugguuaaac caaaaacgag guuauaaauc aagccgauca      420

gacgcaaauc uagggaaaaa ggauacagaa uauguagcca ccguaaaauc acgacaugaa      480

aaaaugaaag agcuagacgu gaccaucgga caauacuuuu aucgagaacu aucagcaaac      540

gaauuuuuua aaguaaaaga aaauauauuc ccuagggaag cuuacauaga agaauuugau      600

gccaucugua aaacacaaca aauagcucau ccaaugaucc uacgaucaga guuaauugac      660

aaaauuagaa acgaaguuau auauuaccaa cgacccuuaa aaucacaaaa aggacuagua      720

ucaguuugcg aauucgaagg cuuuuggcga auaggaaagg acggaaagga auacuucgua      780

ggucccaaag uggcaccgaa auccaguccu cuauuccaau uagcaaaaau gugggaaaau      840

auaaauaaua uaaaaaucgg cucgaggaca ggagaagacu uaaaauuaac aacggagcaa      900

aaaugggcaa uauuucaaca ucuagacaac aaugagaaau uaacaccaac agaucuacuc      960

aaaauacuaa aucuaaaaaa ggaaaauugc uauguaaaca aacaaauggg caaaggacuc     1020

aauggaaaua uaacaaaaca ugcaauccua caauguuuag gaggaucuga caaauaugaa     1080

aacuuacuaa aacucgagcu uaaaauggug gacggugaua aagagggaua ucuuuacgac     1140

agaaaaacag gcgaaguauu aaauuccaaa augaucaaau ucauagaccc caugguagag     1200

caagaaccau ucuaccaacu auggcacaua aucuacagua uacaugauca agaagaaugc     1260

ucaaggguau uacaagcaaa auucgacaua gaacaaccaa ucgcugauaa auuagccgca     1320

aucgauuuua gcaaacacgc auucggaucc aaaucagcua aaguaauuag aaaaauacua     1380

cccuaccuaa uggauggaga cggauauagu gaagcaauga gcuaugcugg uuacgaccau     1440

ucaaauuccc aaacuaagga ggaaaauuua caacgaaaau uacuagaccu auugaaaccu     1500

aucccuaaaa acucacuuag acaacccaua gucgagaaaa uauuaaauca aaugguaaac     1560

guagucaaug caauuaucga aaaauacggc aaccccgacg aaaucagagu agaacuagcc     1620

agagaacuaa agcaaaguaa agaagaaaga aaugaugcgg auaaagcaau gucaaaaaga     1680

caaagagaaa acgaugcaau aaccaaaaga cuagaagaau ucggauuaag ggccacuagg     1740

aacaauauaa uaaaauggcg acuguaugaa gaaauagaca accaagaaaa aaaacuaaac     1800

gcaaugugcg uuuacugcgg acaaccaaua ucccucacag aagcgcuuag aggaaaugau     1860

guagauauag aacacauaau ucccaaaagc aaacucuucg acgauucaca aaguaauaaa     1920

accuuagcuc aucggcacug uaauaagaau aaaaaugacc uaacggccua ugacuucaug     1980

aagggaaaac cagaggcggu auucaacgaa uacguagaga gggucaacuu gcucuaugau     2040

aaucacauaa ucagaaaagc aaaacgagau aaacuacuaa ugacugaaag caagauacca     2100

gacaacuuca uugacagaca auuaagagaa agccaauaca uugcaaaaaa ggcucgagag     2160

auacuucaaa caauaugcca ugauguaugg uccacuucag guacagugac ugcggaauua     2220

cgacacuugu ggggauggga ugauguaacu augaacuugc aaauggcaaa auauagagaa     2280

uuaggacuaa cucaccaagu agaaugggaa ucagaacacg gcaaaaacaa acaugaaaaa     2340

gaaguaauca cggacuggac caaacgagau gaccaucgac aucaugccgu cgacgccuua     2400

acaauagccu guacaaaaca aggauucauu caaagauuca auacauuaag cgcagcuaaa     2460

accagagaag acaugauguu cgacauagaa caacgcuccc augaauucaa ggaaaaaaga     2520

aguuuauugg aaaaauacau aauaucagaa caaccacuua aaguugacga aguggaaaag     2580

gcugucucca auauacuaau caguuucaag agcggaaaaa aagucgcagu auggggaaca     2640

agaaaaaucg guaaacgggg gaguaaaaag guaauucaaa aaaaaauaau uguaccaaga     2700

gggucucuua gcgaagaauc aguauaugga aaaaucaaaa aacaagaaga gaaagacuau     2760

aaguaccuau ucgaaaaccc gcaacuaauu uucaauccaa caaucaaagc gaagguagag     2820

gaaagacuuu uaauguuuga cggagacguu aaaaaagcaa uaacuucucu uaaaaaaaua     2880

cccauauacu uagacaacaa caaaacaguu guauuagaaa aagcgauaug cuuaaaagac     2940

gaauucguaa uaaaauauuc cguagacauu aauuucaaca aaguggauaa aguuguagau     3000

ccaaaaauaa aagaaaucuu acaaaacagg cuaaaaaaau uuggaggaaa agaaaaagaa     3060

gcauucaaag acguccaauu agaggacaaa augauaauua aaugguacga agaugaaggu     3120

cuagagagac caauaaagag cguacguugc uucacgggac uaagugccgu aguaccuguc     3180

aaaaaagaug aaaacggaaa cgacaucggu uuugucaaac caggaaauaa ccaucacaua     3240

gcaauuuaua cagacaccga agaaaauaag caacaacaug uaugcacguu cuggcaugca     3300

gucgaaagaa aaaaauauga cauaccuguc auaauaaaaa auaccaauga agugugggau     3360

aaaauucaaa gccaacccga cgguacauau cccgauaguu uucucgaaau gcuaccaccg     3420

ccaaacuuaa accuauuggu aaauaugcaa caaaaugaaa uguuuauauu agguaccaca     3480

acagaagaua uuaaacuagc cauagacaag agggacuaca aaauaauagg agauaaacua     3540

uauagacuac aaaaaaugag caucaaacca ucuucuggac agauagacau aguauuuaga     3600

caucaccuug aaacacaauu aauagaugau gaaaauagca aaaaaagcaa aagauuuuac     3660

aaugugcaga guuuaggggc ucuuuucgca cugaacccau uuaaaguuag aauagacaau     3720

uuaggaaauc ugguaaagga aaaauga                                         3747


<210>  5225
<211>  3762
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5225
augaaagcaa gaggaaauuu ucuacaucaa gacacaccug gacuaacagc agcaaaugca       60

aacaugggcg augcaguuga aaaauuaugu guagccuuag aagauuggug caccuuacgc      120

aaccuugaau guaauauccg uaaaaaccag aaaaacauac aaaaucuuuu agaaggaaaa      180

accguugaag ucucagcaga aaaugcuuua aaagagucgu ucgacucaag ccgagccagu      240

agaaaagaca aagcaacaau auuaucaaca uggauagcca uacaaccagg cgaguacuua      300

gacgcuaagc aagcuaagga ugcuucaaaa gagauagcca auguauuguu uggacuacaa      360

ggcaauauga agaagauauu cauugaguuc gaagaagacc aaaagauucg uuuagauaac      420

gacgaacaag uggaagcauu uuacucauua ugucccgacg augguguugc auuauuugag      480

ucauuccaau cgauauauag ugcauauauu uuaaugggua uccugaaaaa cgcaaauggu      540

gguucaauau caucaugcaa aguagcagcc uaugaccagu auaaagaaga ucuaaaauua      600

cuaaaagacu uaguaagaga auuugccucu gaaaaauacg augaguucuu ucguggagau      660

auauuugaca guuccaaagg auucagggca agaaauuaua auccagcaac agcaaaagga      720

uacaccaaau acaauagugu ucgcggcgca uccuaugacg auuucaaaaa ggaagucgag      780

aaaaucuuua aagguacagu ugccgaagaa gauauccgau auguugauau gaugcagaga      840

uucgcagaag guacguuuuu gcgacguuua aaaaccucug auaacgggag cauaccauac      900

caauuacauc uagaagaaau ggaugcaauu guagaaaauc aaaaacaauu cuauccguuu      960

uuagaaagug aaaaggaaaa acuagaauca uuaguaacau ucaggauuuc auauuacguc     1020

gggccacuaa cacagaaaaa ugcaagauua gacaaccaca auaaugcaag auuugccugg     1080

agccaaagaa aggaaggcaa ggaacaugca agaguuuacc cauggaauug ggaagaaguc     1140

auagacagaa aacaaucagc agcaaaauuc auggaaagau ugacagguac augcaccuau     1200

auauuagaug aacccguacu acccagaugu agcuuauuau acgaagaaua cugccuucuu     1260

aaugaacuaa auggauccaa auggagagac augggaggag auuggcauag guuugacuac     1320

gaaacaagaa uggaaauuau agaaaaucuu uuuaaaaaga agaaaggauc uguaaaauau     1380

gcagcaguag aggacuggcu gaaaugcgaa aaacacaaau caugcgcaga aguaaaagga     1440

ggucaaggug aaacaggauu cgagagcaaa cucucaucuu auguuuucuu uaaagaaguc     1500

cuucacgucg augaaauaga cgaggcagau auuccaauga uagaagaacu aauauggugg     1560

aacacacugu uugaagacag gucaauccuc aaagaaucua ucgaagauaa guauggagac     1620

agacuuacgg augaccaaau aaaggccaua ugcaagaaac guuuuacugg augggggaga     1680

cuaagcaaag aauuauuaug cggaauaaaa acgcacacag auaauggacc cuacaguaua     1740

auggacguau uacgagaagg aaauccuaau aauaacgcac gaucagagac uaugguauuu     1800

auggagauac uaagagauga caaauuugga uucgauaaau uaaucgaaga agaaaacaug     1860

aggagaguuu cagaaguaga caauuuugac guuaacgauu uacccggaag uccagcagua     1920

agaagaaccg ugaaccaagc aaaacgaauc guggacgaga uagugggcau agcaggaugu     1980

gaaccacaaa auauuuuuau agaaguaaca cgcgaugagg auggaagaaa caaaggucga     2040

cgaacuaaaa gaagauauga caauaucaag aacgcaauag cagcauugaa gaaagaacaa     2100

aaagaguauu uuaacaaaga uguaucagac agcuuaaaua aaacuaaucc uagugaccua     2160

gauaaugaaa gauuagcacu auauuuuaug caaaauggua aauguaugua cuguggaaaa     2220

gauuuagaua uuaaacaacu ugacaaauac caagucgauc acauuuuacc acaaucauac     2280

guaaaagacg acucauuuga aaacaaggcu uuaguacaua uggaagacaa ccaaagaaaa     2340

ucggcaagca ugcuauuaga ugauaguaua auaagaaaaa ugaaacccua cugggaggca     2400

cuaagagcug caggcuuaau aggagaaaag aaauucaaaa aucuaacccg aguuagagug     2460

ucagauaaag aacuuggagg uuuuauagca cgacaaaugg uagaaacauc ccaaagcaug     2520

aaacuaacuu cguuaguacu ggagaaaacu uacccaaaua caaacguacg accuguuaaa     2580

gcagguuucu caagugaccu aagaaaacga uuagauuuaa uuaaaugccg agaaauaaau     2640

gauuaccacc augcacacga cgcguauuua gccugugaaa uaggacaauu cauaacgaug     2700

agacauaaua aacuuuauga aaacccaaua ucuuacucac augcaguaaa agauuuuaua     2760

gcgaaaagag caaaauaugc aaaugaccau gguacuccca uguccacaua cggaaugcca     2820

ggauccuccu cauucguuau cucaucauuu cucacaagug guuuugacga agaaacagga     2880

gaaauauuua aagacgccug gagugccgaa guagcagcag uuagaauuag agaaucccug     2940

aaccgaaagg acuguuauau uagcagaaug cccgaagaaa caaguggugc auuuuggaaa     3000

gaaacuuuaa uuucaccacg cgacccuagu cuagggaaaa accucagccu uccuauaaag     3060

aaaggacuag acccacggaa auauggagga uauaaugaac aaaaauuugc cuauuucuuu     3120

gucuaugaag cagugaaaau uaaaaagaaa ggagaaguaa aagaauacgu auuugcagca     3180

gucccaguac aaguugcagc cauaguagca agagacccca augcacuaac aggauucgca     3240

aagaaagacg ccgaagcacg aaaaauaaaa uucuuacgaa uaaucagaga aaaaguuuac     3300

aaauaccagc ugauagaggu aaauggagag cgauuauaua uuacaggcuu aaaaagugua     3360

agaaacgcaa gacagaucgc guuaucgcaa gauaugacga caguauuccu aaaagcaauu     3420

aacgauaaag augucgcaga agacgaauua aaaagccuuu ucgaagaaau uguucaaaga     3480

uauagauuau gugcaaauag acugggaaac caacuaaaaa uauuuucauu aucagauuca     3540

uucaacucug caaacuccga aagcaagaaa agagcauuag ucucauuagu ugcaauaaua     3600

gacgcaaaaa caaacaaaau agauuuauca gguauuggau cagguaaagc aguaggcaac     3660

augagauuau cuuuuuccaa agaaaugagc aacucgaaaa aucauuuugc cuuuauagac     3720

caaucuguaa caggaauguu ugagagaaaa accuaccuau ga                        3762


<210>  5226
<211>  3777
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5226
augaccgagg aaaauccaga cauacgcaaa guuuuucuug cgauccacca cauacuaaaa       60

aacagaggac auuuccugcu ccaagggcaa ucuuucaaag aaggaaaccu gaauaacuua      120

auaaaagaau uacugaaccu agacaucuug cacaucucaa uagaaaucac agaagaaaau      180

auaaauaaaa uaaccgaaau agcccuagag aagaaaacau uaacagacaa aguuaacgac      240

cuaaaaaaaa ucuauccuaa agaaaagcaa uuacuagaag cauuuagacu aauuuucgga      300

ggcaaaacaa gucuggacaa acuauucgcc auugaugaau acaaggaauu ggacgcaagu      360

guaaaaagug uaaguuucaa agaaaaaauu uacgaagaag uaagacauga uuaugaacaa      420

gugcuuagua auuacauuga gcuacuagac cuagcuaaau ugguauacga uuccauuaua      480

cuaucugaca uuaaaaagga aggaaaaacu cucagcgagu caaaaguaga acuauaccac      540

aaacacagag aagaccuuac aaaauugaag accuuaguaa aaaacgacag caagcuaagu      600

gaagauaaga aaguagaauu auauagugca auuuucaaag aagacaaaga caaaggaaca      660

aacuauguga auuacauucg aaaaucggaa gagggaaaag gaugcaacua cgaagacuuc      720

aaaaaauuuc uaguaaaaga gcuagccaaa cuagaagaaa gcucagcaaa agaagaaaua      780

cucagagacu uagaauuaga guuauuucuu ccccuacaaa gaaccaaaga uaauagugua      840

guaccauacc aaauacacaa agaagaacua guaaaaauau uagacaaugc cgcuaaauac      900

cacaaauucu uaaacgaaaa agacgauagc ggauauucaa uaauagaaaa agucauccag      960

cucuuagaau uucggauacc cuacuacgua ggcccacuua auuccuccaa aaaagcuaag     1020

guagacggau uugcaugguc aguaagaaac aaaggauacg aaaacacccc cguaacaccu     1080

uggaacuaug aaaaagucau cgaugaaucu gcaucagcag aaaaauuuau caccaaccug     1140

acuaacaaau gcacauaucu aaaaggugaa gacguacuac caaaaagcuc acuacuauac     1200

ucagaauuug cacucuuaaa ugaauuaaau gcacuaaagu acgacggaaa ccgaauuucu     1260

cuagaagcaa gaaauguaau aauagaaaaa uuauuuaaag aacaagguaa aaaaguuacu     1320

aaaacuagua uaaaaaaucu cuuaaaagca gaaggauaca uagaugguaa aggagaaauc     1380

acuggaauug auaucacugu uaaaaacgau cucaaguccu aucgagacuu caagaaaauu     1440

cuaggugaca aguucaacac agaacacgug gaaaauauua uucuauggau aacccuauac     1500

ggcgaaucac gaaaacugau caaggcaaaa auagaagcag uauauggcga caucuacucc     1560

aaagaagaaa uagccaaaau gaguagauua guauacaaag acugggguag auucagcaga     1620

aaacugcuca cugaacuugu aucuaaaaag uuauacaaug aagaaacagg agagugucuc     1680

aacaucaucg gugcuaugcg acaaaacaau auccuauuca uggagcuacu agccgaccgu     1740

uuugacuauu cacaacaaau cauugauuau aacaaagaac uucaaaaaga gguuacagaa     1800

auaacaccug aaaucuuaga cgaccuauau guaaguccgu ccguuaaacg uucaauaugg     1860

caaacaguaa gaauagucga agaacuaaaa aaaaucaucg gaugcgcacc agcaaaaauc     1920

uucguagaaa cuacaagauc gaaucaagac aaaaaaaaac caacugauag ucgaaaaaaa     1980

caauuagaau uguuuuauaa ggcaguaaaa aaagauguaa aggaucucga aaaagaaauu     2040

ggaaguuuaa acuuugacaa acuaaacgaa cgauuaaguu caguagaacc aucaaaauua     2100

aaagcaaaaa aacuauacuu auauuacaca caacuaggaa gaugcaugua cagcggugaa     2160

acaaucgaua uaacacaacu aaauaccacu gcuuacgaca uagaucacau cuacccgcaa     2220

ucaaagguaa aagacgacag cuuuaccaac acuguacuag uuaaaagaga auacaaugcu     2280

gcuaaaacug acagauaccc auuaggauca gauauacaaa ccccaucaaa cagacgauuc     2340

uggaaauuuc uuaaagaaaa agggcugauu acagaugaaa aguauaauag gcuagugagg     2400

acagaagaau ucagcgauga agaacuaaca ggauuuauag caagacagcu uguagaaacu     2460

ucacagucag ucaaggccgu agcggggauc cuaggugaac uaaacccaaa aacaagcaua     2520

uguuacagua aggcagagaa uguauccuca uuuagacaga auuucggaaa aauaaaagag     2580

ggaaaucgaa agucagauaa uacggaacaa uuaaucaaag ucagagaaau caacgacuau     2640

caccacgcaa aagacgcaua cuuaaauguu guaguuggua auguauauga uacuaaauuc     2700

acucgaaaug uauuuaauuu caucaaagau aaaaaagacg gaaggaaaua cagccuaaac     2760

aacgucuucu acgaaaacgu aaguaacuca aauaacgugg caugggaauu aaauaaaacu     2820

gugcaccaag uugaaaaggu aaugaauaac aauaauauuu uagugaccag aagaacuagu     2880

gagcaaaaag gaggccuauu cgaugcaaca guauacaaag caaaaguagc agcaaaagca     2940

aaggaaggag uuuacuaucc acuaaaaacu agugauccag uaguaaauga cgugacuaaa     3000

uauggggguu uuacaaaggu uaagauugcc uauuacucga uauuugaaua uacacuggua     3060

aauaaaaaag gugaagaaaa aaucacaaga aucauaccua ucccaauaua cguaucacaa     3120

aacauuaaag acgauagcac uuuauuagaa uucggcaaaa cacaaauaaa cauaaaauca     3180

ggggaagaag uaaaagauuu uaaacugaaa uauaggaaac uaugcauagg cgauuuagua     3240

aaaauaaaca auuuuuuuaa uuuuauuggu ggaaaaacaa augacucauu cgucuaugac     3300

ggagcagucc aaguaacuuu agacaccgag uccgaaagau acauuaaaac uuuaucgaaa     3360

uaccaaaacu ggaagaaaga aaauaaagau ggcgaauuau gggagucaau aacuaaagag     3420

aaaaacacag aauuauacaa ccuauuagua gaaaaaauga auucgggaau cuucauaaaa     3480

aaaaaaccaa acaaguucaa cgaauuaaau ucagaagaga uaaggaauaa cuuuuccagc     3540

aucagccuag aggaacaagc aaaaguucuc cuagagguac uaaaccuccu cacuaacaag     3600

aaaucaauau uugauuugaa aucgauaaau aucaccgcgg caagaggaaa aauaagcuuu     3660

aauuuaucaa auuuaaguca auucagcauu auagaacaaa gcguagcggg cuuuuaugaa     3720

aaagaaauaa cgauuauugg agacaaagaa aacgacaugg aaaacaauaa cuguuga        3777


<210>  5227
<211>  3780
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5227
augaaauaca uucuuggucu agaucuaggg guagcgucua ucggaugggc agugacggag       60

auaaacgaaa aaggagagcc gguaggacuc auugacgcaa auuccguaau cuucaaaccc      120

cucgauaacg auaaaggaaa acuauauaau gucgaaagaa gagacaagag agguucaaga      180

agaauacuac gaagaaagaa acauagaguu gaaagaacaa aaauguuacu cgugaacuca      240

ucuuucuuaa cuaacgaaga aauagauaau cuauaugucg gaaaauuaga caacaucuau      300

gaaguacgau uaaaaggacu caaagaacaa cuaaguaaaa augagaucgc aagacuuaug      360

auauauuauu gcaaaaaucg uggauuuaaa ucaaauagaa aaacagaaga cuuagaaaua      420

uuaaaaucac uaggcgagaa aauaucggaa aaagacucag acgaaaagaa acuaaaaccc      480

auaauagcca aaaacacaga acuaauagaa agcaaaggac uaacacccau cgaagucaua      540

uacaugauua gagaaaauga caacuccaua cugggauuua aaaacaaaga aggcaauuau      600

aaauucggcu ucaaaagaaa ucaaguaaua gaugaaguga gaaaaauuuu aggaacucag      660

caaauccuaa gcaaagaaau aguagacgaa uacauagaca uauuaucaag ccagcgagac      720

uucucagaug ggccaggggg agacucaaag uacaagauag acuacacaaa guuagcagga      780

aaauguaagu acacagguga aguacgcgca guaaaaagcg cuccaucuua ugaaauauuu      840

acgaugcuac aaaagcuaaa cgacauaaga uauguaaaau uuacaagcga agacaaaauu      900

gaaaagaaga aacuuagcaa agaaguaauu cauaaacuuu augacuuagu aguagagaaa      960

aacaaaaccc uaacauacga ccuaauagaa aaaucaauag augaagaaaa cauuaaacua     1020

cugaacauac ccaaauuauc aaaaagcaaa uacguagaac uaaggaaauc uuauuucaaa     1080

gaacgcaacg auaacgaaaa uuuaaccgaa gaagaaauaa augcauuuaa ugaaaaauua     1140

aacaaugaaa gaaugaaaca agaacuaauc cgaaacaauc uaaaagcaua caaagaacua     1200

aaaaguaaau uuaagaaaaa uaauauagac gagaacagga uaaaagaacu aggaggcauu     1260

uauuuccugg auuuaguagc ggaacuccuu acguacagua aaacggacga aaagcuagaa     1320

uacuucgugg aaaauguaga acgauauagc cucuuuaaag aacaacguga cauuguugaa     1380

auaauaaaaa cuuuccccaa uuauacaaag aacggcaacc uaucacuguc uuuaguacga     1440

gaacuaaaca aacuuuuaau ggagggacac gauuaugagu cuagcuuaag uagccucaac     1500

uauuacaucg acacugaugu agacugggaa aaauucccaa caauaaguga aauugaagac     1560

ucccuaucca caaaaauaac aaaucccaau guuaaacaua ucuuaguaau auuaagaaaa     1620

uuauacaaca cucucuuauu uaaauaugga agacccgaaa agguacaccu agaauuauca     1680

agagacuuua guaacgacuu uagcacaaga aacaaaauuc aaaaagagca gcuggagaau     1740

aaaguaagaa gagaaguggc cgcguucgaa auguauggcg caaauaaaga caucguugca     1800

ggaaaagaca ggcuaucgaa cgacgauuuu guacgcauaa aacuauggga agaacaaaac     1860

aaggugugca uguauucagg aagaacaauu gaaaaauacc aguuaaccuc agcugaggug     1920

caaauugauc auauccuacc cuacagcaag agcuucgaca acagcuacag caacaaaguu     1980

uuaguuuuua gcaaugaaaa ccaagauaag aaagaaagga cgccauauca gugguuaaaa     2040

gggacugaaa aauggaauga guucaaacaa aggguccggc uaaaccugaa cauaucgaac     2100

aaaaagaaag aaaacuugcu auucgaagau gaaguaguaa auaaugaguu ccuagaacga     2160

gaacuacaug cuacaucaua caguucaaga cuagcccuaa auauuuuuca aagacucaua     2220

ccagucuccg acgaagacaa auacgacgaa cauggcaacg aaaaagucaa auaucuguau     2280

aacaggaacg ucaucgcauu ucaaggcaaa augacaucga uguugagaaa ccuuuacuca     2340

cuuaacaaau auacucacuc guucgaaucg gauacacuag acaucgauaa uaaagcauuu     2400

auacuuaaag aguucgaaau aaauaaagau aaucuuaaaa ugacagcuua caacgugaau     2460

accgggcuug aaauaacuuc agaaacaaaa guagagaaaa auaaaucagg agaguucaaa     2520

acaaucaaag augaacuacu aaagaaagaa uuagauaaga aagaaaauuu aauagaaaua     2580

ucagauuacu ucaaagauaa aguccucuuc gaucuaaaaa uaguagauuu ugaagaaaua     2640

gacacaguag aaccagaaau caucucgauc cuauacaaaa ugauaacggc gcucaaagaa     2700

gaaguauaca gcaaaaacag agaaaaccau cuucaucauu cacuagaugc auucuuacua     2760

accauaauga acagaucaau gcaaaugaaa uuaaucaaau acaaucaacu aauaagcgua     2820

uuaaagaaca aagaaauaga gauauuuaac gaagaaaaag gugaauauau agauaguaaa     2880

gaauuugcag aagaacuaca aaaacagaaa auaauagaca uagacggaaa cgaaagggga     2940

aucaaauuca acguaaacgg uaaaacacac cgacuuaacu uaguaaaacc uuacgagaac     3000

uucuuagacg acauuaaaaa caaaauauuu gacaaacgcg aaaauaacaa acuuccguac     3060

cacguaguaa aaucgaaagu cagcggggca uuacacgcug aaacaauacu aggugaaucu     3120

aaaggagaaa uaacaaagag aauaucaguc uucgacauaa acuacaaaaa ccuagguaaa     3180

auauuugaca aagauggcuc ccaaaaagaa auauacgaaa cauuaguaaa auggcuagaa     3240

gcuaaaagua aggacuaccc caaguuaaag aacggaaaca uaaucaagaa aguaaaaaua     3300

guugacggaa auaaagacaa acuaaucaaa cuaggaaaca aacgauaugu ggaaaugggc     3360

agaacaacgg uaaaaaucuu gguacuuaaa aaggaagcag aagaaggacu aaaguuugca     3420

agcauaggua gauacaaaua uaaccuacua aaaagcgaaa aagacguuaa uauaaguaua     3480

uggaaagacg ccauucacuu uugcaaagua cgauauaaca aacuaaagga aaacggcuac     3540

agguuaaucc acgaacuuau accgggagaa acgauagagu uagaacuaaa uaaaggaaac     3600

auaucaaaau gucuugucgu aggauuuagu ggaggaaaaa uagaaauauc aucagucaua     3660

ggagacgcac uagaucugau aaacgauaaa auauccacca gaauaaaaga aagauauuau     3720

aucacagucu caacaauaaa guccaucaag aaaaucaaca aaaacauauu aggagauuga     3780


<210>  5228
<211>  3792
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5228
augcauaaaa acaaucccga caucagagaa uuguucuuag caauacacca uauauuaaaa       60

uauagaggac acuuccuauu cgcagggcaa caauuuaaag guucagaaau uaaagauaau      120

aucagagaau uauuacaaua uaacagccua aauauaaaaa uagauauuac agacgaauug      180

auaaauaaca uagcagaaau acuccucaaa aagaaaaacu ccacggacaa aauaauagaa      240

uuuaagaaac uuuucgcaga cgaaaaacaa cuaauagaag cacuacgucu cgcacuaggc      300

aacaaaacac aacuugcaaa acuauucgac auagaagcau auaaagaauu agauauuuca      360

auuaaaacaa uuagcuuuaa agagaaaaua uacgaagacg uaagaagcaa cuaugaagaa      420

auuuugggag aauacaucau agugcuagau acugccaaac uaauuuacga cgccaucaua      480

cuaucagaaa uaaaaaucaa aaauaugaca auaucagaau cgaaaguaau aagcuaugaa      540

aaacauggaa aagacuuaaa acaauuaaaa caacuaauaa aagaagacuc aaaacuauca      600

aagaaagaga aaagacaguu auuugaaaac auauucguca aagaagauga aaaagaagca      660

aacuacguaa acuacauagg caaaaauaau gucggaagca gcaaaagcuc cuguucauau      720

gaagacuuua agaaaagccu aauaaaagaa cucaaauacu gugaagaauc aaaacucaaa      780

caagauauac uugcagaacu ugaacuagaa acauuuuuac cacgccaaag aacuaaagac      840

aauaguguaa ucccauacca aauacauaaa gaagaacugg agaaaauacu gaauaacgca      900

guucaauacc auccauuucu uaacaauacc gacgaauacg gcaucucaau aaaagagaaa      960

auuauucaau uacuagaauu uagaaucccc uauuaugucg guucuuuaaa uacaucacau     1020

aaaguaggca acggaggauu cucauggucc guacgaaaca aaggcuacga acguacagca     1080

gucacaccuu ggaauuucaa uaaagucaua aauaaagaag aaucggcgaa aaacuucaua     1140

gaaaauuuaa cuaaccaaug caccuaccuc cuauaugaag auguccuacc uaaacauuca     1200

cugcucuauu ccgaguucac ucuauuaaac gaacuaaauu cuauaaacua cggaggcaaa     1260

agacuaacug ucgaacacag aaaucuaauu auagaaagau ucuucaaaca auccgcaccg     1320

aaagccaccg gaaaagaacu gaagaaauuc cuaaugagug aaggagucau cgacgggaaa     1380

gaugaauuaa gcgguuucga caucucaauu aaaaaugauu uaaaaucaua uagagauuuu     1440

aagaaaauau uaggagaaaa auucgacagc gaaaaagucg agaaaaugau aagauggaua     1500

acacuauucg gcgaaaguaa acaacuucuu guggacaaaa uaacgaaaga auacggcgac     1560

aucuauagcu cagaggaaau caagaaaaua ucagggcuua ccuacaaaga uuggggacgu     1620

cuauccgaaa aacuccugac ugaaaucguu ucaucacagc uuuacgauga aagcaccgga     1680

gaauguuuua auaucauuaa cgcaaugaga aacaacacac uacuauucau agaauugcuc     1740

gguacaaauu acgauuacuu cgagaaaaua aacaccuaca auggaaaauu aaggaaagaa     1800

auaacagaaa ucacaccgca uauacuugau gaccuuuacg uaucaccagc aguaaaaaga     1860

ucuaucuggc agguaguaag gaucguagaa gaacuaaaga aagugauugg cagcgaacca     1920

aaaagaauuu ucguagaaac uacacgcagc aauaaagaaa agaaagguga ugacggaaga     1980

aaagacucaa ggaagaaagc acuacuggaa cuauacaaga aaauaaaaac aagugauaua     2040

gcggaauuag aaaaagcccu cggucacaca guugacuucg auaagaagaa acaagaucua     2100

accgacauag acaacaauaa auuaaaagcc agaaaacuau accucuauua cacacaacug     2160

ggacgcugcu uauauacagg caaagcuaua gauuuaaaua aucuauucgg cucaaacagu     2220

gaguacgaua uagaccauau uuacccacaa ucuaaaacaa aagauggauc cuggaauaac     2280

cuaguacuug uuaauaaaac agaaaauucu aagaaaagua acggcuuaau aagugaaagc     2340

auacaaagga aaaacaagca uuucuggcga uuucuaaaag ccaaaaaccu aaucucagaa     2400

gaaaaauaca aacgauuaac acgaaccgaa gacuuuacug auggagaacu cuccggauuu     2460

auagcuaggc aacuuauaga aacaucacaa ucagucaaag ugguuagcaa aaucuuacaa     2520

aaccucaacc caacuacaga auuauguuuu ucaaaagccg aaaauguauc agacuucaga     2580

caagaguucg guaaaaucaa aguagggaaa cguaaaaacc uaaccgaaga agaacagaag     2640

aaagcgaaag agggacaauu aauaaaagua agagauuuaa augacuugca ccacgcaaaa     2700

gaugcauacc ucaauauagu aguaggcaac guauaccaca ucaaauucac gagaaacccc     2760

aaaaauuuua uaaggaaaaa cuucaaaaac cgcgaauaua ccuuaaacac auuauucuau     2820

ucagacaaag uugaaagaaa uggugaagua gcaugggaaa aaggaaaaac cgaagaaauc     2880

guuucaucag uaaugugcaa caaccuaauc aacguaacac ggagaacaag ugaacaaaaa     2940

ggagcccuau acgaagcuac uauauauaaa gccaacgcag caaaagaagg uucauauuac     3000

cccgucaaaa ccaacaauaa gaaccucaaa aauguaacac gauacggagg uuuuaccucu     3060

auuaaaaucg cauacuauuc aauauuuaag uaugacauca gaaacaagga uaaaguuaag     3120

gagaauauaa aaauuauacc aauaccgauu uauaucuccc aaaacauaaa aacagauuua     3180

gaucuaauaa auuucgcaga aaagcaaaua aaauuaaaga aaggugaaaa uaucgaaaac     3240

uuacaacuaa uuuacaagaa acugaacauc ggagcgcuag ucaaaauaga ucauuaccca     3300

uauuauaucg gcggaaaaac agacgacaac uucuacgucg auggggcagu acaaguacua     3360

cugucuucag acauagaacg uuauuuaaaa gaagugguga aauucgucaa cuuucaaaga     3420

gaaaacaaag aagcaaaagu aucgaaagaa cauauaagcc ucgaagaaaa cauaaaacua     3480

uauaacacuu uaguauguaa gauggcaaca aaaauauuua cccauaagaa aaauaauaaa     3540

uauaacgacc uaacacaaga caaagccaaa aacacauuca auaaacuuuc auuagaagaa     3600

caaugcaaag uuuuauuaga aaucuuaaac cuauuaacaa auaagaaaac cacauucgau     3660

cucucccuaa uuaaugagaa aacaucacgc guuaaaugcg gauucaaucu auuaaaucaa     3720

aaacaguuua gcaucauaga acaaagugua acgggauuau acgagaaaga gaucaaaaua     3780

aucgaagacu ga                                                         3792


<210>  5229
<211>  3795
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5229
augaaaaaug uacuaggauu agaucuaggg acaaacucua uaggaugggc auuaauagaa       60

guugaugaaa acaaucuacc aauuagaauu auagcaaugg guucaagaau aauaccacua      120

ucuacaagug auaaagaaga guuccaacgc ggacuaucua uuacuaaaaa ccaaucaaga      180

acuguaagua gaacgcaaag gaaaggauac gacagaaaac agcuuaagaa aucagaucuu      240

aagaaaauac uaaauaaaua uaauaucuuu ccuucagaag aacuacuaag auuaccaaug      300

uuagaucuau ggaaacuaag aucagacagc gcaaacccag acgaaaacau aucugcugaa      360

caguuaggaa ggauacucua uaugcuaaau caaaaacgag gauacaagag cgcgaggucu      420

gaagcaaaug cagauaagaa agauucagac uauguucaag cuguuaaagg cagauaugac      480

caauuaaaag aucgcaaaca gacaauagga caauauuuuu acgaugaacu uuauaaugca      540

aacgcaaaca acaguuauuu ucggauuaaa gaagaaguau acccaagaga agcauacauu      600

gaagaguucg acacaauaau aagugcucaa cgagaaaaac augauuuccu cacagaugaa      660

guaguccauc aacuaaggaa ugaaauaauc uacuuccaga gaaaguuaaa gagccagaag      720

gggcuagucu caauuugcga auuugaaggc uuugagaaaa caguauuuga uaaagagaaa      780

aacaaaaaua aaguaguauu cgugggcccu aaaguagcuc cuaaaacauc accacuuuuu      840

caacugugca gaauauggga aguuguaaau acaaucacac uaaaaaucaa aaacccagaa      900

ggaagcaaau acaaaugggg agagaaaaua cccgacauaa aagaaaaaga aauccucgcu      960

gacuauuuau uuaaaaacga aaguuuauca uucucaaaac uucuagaaau auuggaacug     1020

aagaaagaaa augucuacgu gaauaaacaa aucuuaaaag gcauaaaagg uaaugaaaca     1080

uacagcgcac uacacucgau acuaggcgac aaugaauuac uaaacuucga cguauccauc     1140

augacaucaa aucagacggc uuuacucgua gacagaaaaa cuggagaaau acuagaagaa     1200

agagcgggac uggaacuuga ugcaucacua gaaaaagaac cacuguacca acuauggcau     1260

acaauuuauu ccuuaaaaga ucuugacgaa ugcaaaaacg cacucauaaa aagauuugau     1320

uuugacgaug aaacagccga aaaacuuagc cgaauagacu ucaacaaaca agccuuugga     1380

aacaaaagca acaaaucaau gcgaaaaauc cucccauucc uaauggaagg auacgauuau     1440

ucccaaucau cgagccucgc cggguauaau cauagcaauu cauuaaccaa aaaugaaagg     1500

gaacagcaga ucacucuaga ccgacucgaa uuacuaccca aaaacuccuu acgacaaccc     1560

auaguugaga aaaucuuaaa ucaaaugauu aauguuguga acgccauccu agacgaauau     1620

ggaaaaccuu cagaaauacg uguagaauug gcaagagaac uaaaacaguc uaaagacgaa     1680

aggaacgaaa cagaccuaca aaacagcaaa aauaagaaau uaaaugacga aauagguaaa     1740

cgacuugaag aauuaggccu uccaguaaca aaaagguaca uacaaaaaua uaaauucauc     1800

uuuccuucuc uaaguaaaaa ucucaaagaa gcaaaaguca auaaccaaug cauauauugc     1860

ggagaaacau ucaauuuaac agaagcacua agcggagaug cuuaugaugu ugaccacaua     1920

guaccaaaag cauuacuuuu cgacgauagu caaacuaaca aaguacuugu acacagaaaa     1980

uguaacucaa caaaaacgaa ccagacagcu uacgauuaua uugccacgaa aggagacgaa     2040

caucuaaaac uauauagcga aagauuagac gauugguucg aaagaggaau aauaaguuac     2100

aguaaaaugc aacgacuaaa aguaucccac aaagaauauu uagaaagaaa gaaacucggc     2160

aaagaaacag aaaccgacaa gaaacuaugg gaaaauuuca uagauagaca gcucagagau     2220

acacaauaca uuucuagaaa auccagagaa auauuacaga aaauaugcaa cgacguaaaa     2280

acaaccgaag gaggaguuac agccaagcua agaaaccuuu ggggauggga cgauguccuu     2340

augaauuuac aaaugccaaa auacaaagaa uuaggacaaa ccauaacuaa agaauggacg     2400

ucagagcaug gcaaaagaaa acaucaaaaa gaagaaauag acaauuggac uaaaagagau     2460

gaucacagac accaugcaau agaugcccua guaauagcau guacuaaaca agguuucaua     2520

caacgaauca acacacuaaa cucuucagac accaaagacc uaaugaucaa agaagucgaa     2580

gaggcaaaaa uugaguucaa cgagaaaaca acauuguugg aaaaauaccu aaaaacccaa     2640

aaaccauuua gcacucaaga aguaaugaaa gaagcugaua aaauccuagu aucauucaaa     2700

gccggcaaga aagucgcuac aaucacaaaa uuuaaagcga ccggcaaaaa uaaagagaca     2760

ggugucauag uaccaagagg ugcauuacau gagcaaagcg uuuacggaaa aauaaaaguu     2820

aucgaaaaag acaaaccacu aaaauaccua cucgaaaaca gcgacaaaau aguaaaucca     2880

agaaucaaaa auuuaauaga aauaaaacua agcgaaaaca aaaacgaccc caagaaagca     2940

uuacuagcac ucaagaaaau cccaaucuuc uuagaugaag aacaaaguau cacauuagaa     3000

aaagcaagcu gcuauaacga cgcaacugua cuaaaguaua aauugcaaaa ccuaaaagcc     3060

gcucaaguag acgauauagu cgacgaaaaa gucaagaaaa uagugaggga aagauuaucc     3120

caauuuggca auaaagaaaa agaagcauuc aaagacacuc ucugguucaa ugaagagaag     3180

aaaauaccaa uuauaacagu gagacucuuc gcuagaccgg acgcaaacaa uuuacaaacg     3240

aucaagaaag augaaaaugg aaacggaaua ggauucgucg uuacagguaa caaccaucau     3300

auagcuauau augaagauaa agauaauaaa ccaguacaac acucaugcac auuuuggcac     3360

gcuguagaac gaaagaaaua caauauucca gcugugauua gagauucauc agaaguaugg     3420

aacgaucuac uaagaaaaga gcuacccgaa ucauucuuaa auaaacuccc agccgacaac     3480

cuaaacuuaa aauuuaguau gcaacaaaau gaaauguuca uacuagggcu aucccaagau     3540

gaguucguaa aagcaauagc aaacaaugau aaaucccucc uuucaaaaca cuuauaccua     3600

gucuggucua ucucagaaaa uaacuacugg uuucgccauc accucgaaac caaaaacagc     3660

gaucuaaaga aaacugacgg cgugaaggaa uccaaacgac uauauaauau acggucauua     3720

gguagccucu uuucacuaaa uccaauaaaa gugcggcuca acuaucucgg agagauaacc     3780

aaaauaggcg aauga                                                      3795


<210>  5230
<211>  3801
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5230
auggcaaaau acauacuagg uuuagauuua gguacaaacu caauuggcug ggccuuaaua       60

gaaguagaug agaacaauau accaauacgc auaaucguga ugggcucaag aauaauaccc      120

cugagcucaa acgauagaga agaauuccaa agaggccucu caaucacuaa aaaccagaac      180

agaaccauuu ccagaacgca acgaaaaggu uacgacagaa aacaacucaa aaggaacgac      240

cuaaauaaag uauuaaacaa auacaacaua uuuccaucaa aaauguuacu gaaucuucca      300

auguuagacc uauggaaacu ccgaagugac agugcgaauu cuaacaaaga uaucagugca      360

gaacaacucg gaagaauacu cuacaugcuc aaccaaaaaa gaggauacaa aagugucaga      420

agcgaagcaa auucagacaa aaaggauacu gacuacguac aagccguaaa aggaagauac      480

gcacaauuaa aagaccgaaa ccaaacaaua ggacaauacu ucuacgaaga gcuuuacaac      540

gcaaacguaa acaacaguua cuuuaagauc aaagaagagg uuuacccaag agaagccuau      600

auagaagaau uugauacaau aaucaauucg caaaaaacua aacacgaguu ucuaacagau      660

caauucguac accaacuacg aaacgaaauc auauacuucc agcgaaaacu gaaaucacaa      720

aagggacuaa uaucaauuug ugaauuugaa gguuuugaaa agacaguauu cgacaaagaa      780

aaaaauaaac auaaaaucgu uuuuguaggc ccgaaaguag ccccaagauc cgcuccucuc      840

uuucaacuau gcagggucug ggaagucaua aauacaauaa gcauaaaagu aaaaaauccg      900

gaagguucua aauauaaaug gggagaacgc auaccaagca uaaaggagaa agaaauucua      960

gcugacuacu uauucaaaaa ugaaguauua ucauuuacca aacuuuuaga aauauuagga     1020

uuaaauaaga acgacgucua cguuaacaaa caaauauuaa aaggaaucaa gggaaacgag     1080

acuuaugcaa cucuauacaa uauucuaggc gagaaugaau uccucaauuu cgacgugagc     1140

auaauacccu caaaccacac uucaauguua guagauaaaa aaacaggaga aauccuagag     1200

gaacgagcag gacuagaauu agacguauca cuugaaaaac aaccauucua ucaauuaugg     1260

cauacaaucu acucacuaaa ggaucuagac gaaugcaaga acgcguuaau uaaacgauuc     1320

gacuucgacg acgaaaccgc agaaaaucuu aguagaauag acuucaauaa acaagccuuc     1380

gguaauaaaa guaacaaaag uaugcgaaaa auauuaccuu uucuaaugga aggauacgau     1440

uacucacaaa guuguucauu agcaggauac aaccacagca acagucuaac aaaagaugag     1500

cgagaacaac aagucacgcu ggacagacua gaacuacugc caaaaaauag ccuaagacaa     1560

ccaauaguag aaaaaauacu aaaucaaaug auaaauuuag uaaacgccau uuuagaucaa     1620

uauggcgaac ucucagaaau aagaguugaa cuagcaaggg aacuuaaaca gaguaaagac     1680

gaaagaaacg augccgaucu acaaaauacc aaaaacaaaa aacuaaacga cgaaauaggu     1740

aaacgauuag cagaauuagg auuaccaaua acaaaacgau auauucaaaa auacaaauuc     1800

auauuuccau cccucucgaa aaaucucaaa gaugcccaug uuaacaauca augcaucuac     1860

ugcggagaaa cauuuaaccu uagcgaagcu cugucaggag acgcguacga ugucgaccau     1920

auuguaccga aggcccuacu auuugaugau agucagacaa acaaaguguu aguacaccga     1980

aaaugcaauu caacaaaaac uaaccaaaca gcauaugacu acaucucaac aaagggcgac     2040

gaacaaguuc gaauuuauuu cgaaaggguu gacgauuggu uuaaaucagg aauaauaagu     2100

uauuccaaaa ugcaaagacu aaaagucagu cacaaagaau acuuagaaag aaaaaaaaua     2160

ggcaaagaaa ccgaaacaga uaaaaaacuc ugggaaaauu ucauagacag gcaacuucgc     2220

gacacacaau acauaucaag aaaaucccga gaaauacuac aaaaaguaug uaauaacguc     2280

acaacgaccg aagguggggu aacagcaaaa cuucgaaacc ucuggggcug ggacgacguu     2340

cugaugaacc uucagaugcc caaguacaga gaauuagggc aaacuguaau aaaagaaugg     2400

accagugagc auggaaaacg aaaacaucaa aaagaagaag uagaaaacug gacaaaaaga     2460

gacgaccacc gacaccaugc cauagaugcu uuagucauag caugcacaaa gcaaggauuu     2520

auacaaagaa uaaacacacu aaacuccucc gacaccaaag auuuaaugcu uaaagaaguc     2580

gaggaagcaa aagucaaauu uuccgaaaag acaacacucu uggaaaaaua cuuaagaaca     2640

caagaacccu uuagcacaca ggagguaaug agggaagcag acaaagugcu agucucauuc     2700

aaggcaggaa aaaaaguugc cacaauaaca aaauucaaag cuauaggaaa aaauaaggau     2760

ucaggaguaa uuguccccag aggcgcccug caugaacaau caguuuaugg aaaaauuaaa     2820

gucauagaaa aagacaagcc gcuuaaauac cucuuugaaa auagcgacag gauaguggac     2880

cccaaaguaa agaaauuagu ugaaguaaga cuaucagaga acgaaaauaa cucaaaaacu     2940

gcaauaucaa cccuuaaaaa agccccaauc uuucuaaaug aagaaaaaac agaaguacuc     3000

gaaaaagcau caugcuacaa ugacgcuacu guacuaaaau auaaacuaca aaguauaaaa     3060

gcaucucaag ccgaugacau aguagaugaa cgaguaaaau uccuaguaaa agagcgacuc     3120

gcacaauuug guaacaaaga gaaagaagca uuuaaagaca uccuaugguu uaaugaagaa     3180

aagcaaaucc cuauaauauc gguccgccuu uuugcaagac cagaugcuaa caaccuacag     3240

guuaucaaaa aagacgaaaa uggaaaguca auugguuucg uauuaacagg aaacaaccau     3300

cacauagcca uauacgaaga uaaagacaac aaacuuauac aacacucaug uacauucugg     3360

cacgcaguag aaagaaaaaa auacaauauu ccaguaguaa ucaaaaacuc gucggaugua     3420

uggucacaac uacuagacaa acaacuacca gaaagcuuac uaaacaaacu accaacagac     3480

aaacuuaauu uaaaauuuuc uaugcaacaa aacgaaaugu ucauccuagg ccugucacaa     3540

aaagaauucg acgaagcaau caaaaauaac cacaaaucuu uucuauccaa auacuuauac     3600

cugguaugga gcaucaguaa uaaugauuac uauuuucggc accauuuaga aaccaaaaau     3660

acagagcuaa aaaauauauc uacugcaaaa gaaucuaaaa gauacuucag auucaaguca     3720

guaggcgcau uaguuggaac aaacccuauu aaaauaagac uaaaccaccu uggagaaauc     3780

aacaaaguag gugaauauug a                                               3801


<210>  5231
<211>  3807
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5231
augacaaaaa auaucuuagg gcuagacuua ggaacaaaua gcauaggaug ggcccuuauc       60

gagguagaug aaaacaauac accaauuaga aucauagcaa uggguagccg gauaauacca      120

cuaagcaccu ccgacaagga agaguuucaa aggggcuuau cgauaacaaa aaaccaaagc      180

agaacaguaa gcagaacaca acguaaaggu uacgacagaa aacaacuaaa aaaaucagac      240

cugaaaaaaa uccuaaauaa guauaauauc uuucccagug aagaguugcu gaagcugcca      300

augcuagauu uauggaaauu acgauccgac agugcaaacc ccaaugaaaa caucagcgcu      360

gagcaacuag gcagaauacu guacaugcuc aaucagaaaa ggggcuauaa aucagccaga      420

agugaagcaa acgcagauaa aaaagauacc gacuacguac aagcaguaaa aggaagauac      480

gcacaacuua aggaaagaaa ucagacaauu ggacaauauu ucuacgaaga auuauauaac      540

gcaaacacaa acaacucaua uuuuaggaua aaagaggaag ucuacccaag agaggcauau      600

acagaagagu uugacaccau uauaaaugcu caaaaaaaua aacaugauuu uuuaacagau      660

gaagugguac accagcugag aaaugaaauc auauauuuuc aaagaaaacu uaaaucacaa      720

aaaggacucg uaagcaucug cgaauuugaa ggcuucgaaa aaacuguuuu ugauaaagaa      780

aaaaauaaag auaaaguagu auucguagga ccaaaaguag caccuaaaac uucaccauua      840

uuccagcuau gcagaauaug ggaaguaguu aauacuauau cacuaaaaau aaagaauccg      900

gaaggaucaa aguacaaaug gggggaaaag auaccaaacc uaaaagaaaa acaaaucuua      960

gcagauuacu uauucaaaaa ugauagccuu uccuuuacaa aacuacuaga aauccuagau     1020

cucaacaaaa acgacgucua ugugaacaaa caaauccuaa aaggcaucaa aggaaacgaa     1080

acauucucaa cauuacauac aguacuagga gacaacgaau uacugaacuu ugacguauca     1140

auaauuccaa gcaaccauac aucgaugcua guugacaaaa aaacaggaga aauacuagaa     1200

gaaagagcag guuuggaguu agacgcauca cuagaaaaag aacccuuaua ccaacuaugg     1260

cauacaauau acucauuaaa agaucuagac gaaugcaaaa augcacucau aaagcgauuu     1320

ggguuugaug acgagacagc ggagaaacua agccauauug acuuuaacaa acaagccuuc     1380

ggaaacaaau caaauaaauc uaugagaaaa aucuuaccau uccuaaugga aggcuacgau     1440

uauucucaau caugcucccu agcaggauac aaucacucca auaguuuaac caaggaugag     1500

agagaacaac aaauaacuuu agacagacua gaauuauuau caaaaaacuc ccuaagacaa     1560

cccauuguag agaaaauacu aaaucagaug auuaaugucg uaaacgcaau cuuagaacaa     1620

uacggaaaau uaagugaaau acgcguagaa cuagcaagag agcuaaaaca aagcaaagac     1680

gaaagaaaug acgcagaccu acaaaauucg aaaaacaaaa aacuaaacga agaaauagga     1740

aaaagacuag cugaacuggg auuacccguc acaaaaagau acauacaaaa auacaaauuc     1800

auuuuccccu cucucucuaa aaaccuaaaa gaugcacacg uaaacaauca augcauauac     1860

ugcggagaaa cguucaaucu aacggaagca cucuccggag acgcuuucga cguugaucau     1920

auuguaccua aagcauuacu cuucgaugau agccaaacaa acaaaguacu aguacauaga     1980

aaaugcaaua gcacaaagac caaccaaaca gcauacgauu acauugggac gaaaggcgaa     2040

gaacaaguua gaguguacuc ugaacguaua gacgacuggu uuaaacgagg aauaauaucc     2100

uacaguaaga ugcaaagacu aaaaguaucu cacaaagagu aucuagaaag gaaaaagcua     2160

ggaaaagaaa cggaaacaga uaaaaaauua ugggaaaauu ucauugacag acaauuacga     2220

gacacacaau auauaagccg gaaagcaagg gagauccuac aaaaaguaug uaacaacguc     2280

acuacaaccg aagguggggu aacggccaaa uugagaaacu uauggggcug ggacgacguc     2340

uuaaugaauc uacaaaugcc caaauacaaa gaacuaggac aaacugucau aaaagagugg     2400

accagugaac acggaaaaag aaaacaccaa aaagaagaaa uugaaaauug gacaaaaaga     2460

gacgaucaca gacaucaugc aauagacgca cuaguaguag cuugcacaaa gcaagguuuc     2520

auacaaagaa uaaacacgcu uaauagcuca gauacaaaag acuuaaugca acgggaaaua     2580

aaggaagcaa aaaaacuacu aggagaaaac uacaaugaaa aagaauucga aaacgacaac     2640

gaauucguca aaaaucacaa agaacgacua ucccaacuag acgacuaccu aagacuacaa     2700

aagccuguca aauuugauac gaaauaugua gaacaagaag ccgacaaagu ccuuguuuca     2760

uucaaagcag gaaaaaaagu agcaacaauc acuaaauuca aagcaacagg gaaaaauaaa     2820

gacacuggcg uaauaguucc aagaggagca uuacaugaag caggacuuua ugguauaaga     2880

acaauauacu caaagggggg cauagcugaa aaacauauaa uaaaaaaaua uaaagucgga     2940

guaagcgcac aggaccauau auucacagga aaggagacau acucagucaa aaaagacaaa     3000

aauggcaaug aaaaagucga ugauaaaaua aaaauagugu uagauucaau aguagacaau     3060

aaaguaaaag aagucguacu aaacagauua aaucgaggcu ucaauaacga agaagacuau     3120

cguaucgacg ugaaaaaggc auuaaauaac uucaaaaacu uagacgaaga cccauugugg     3180

uauaaugaau caaaaggaau caaaauacua uccauaaaac uaaaaacagg ccuaaaggac     3240

ucaguaguac cacuacgauu aaacgaaaag aaugaaguaa uaacccuagu caaaacaggu     3300

aacaaccacc auauagcaau cuaugaagac aacgauggaa aacuaauaca auauaguugc     3360

acguuuuggc augcgguaga acgaaaaaaa uauaauaucc ccaccguaau aaagaacagc     3420

ucagaaguuu ggaaucaagu acuagacaaa aaacuuccag aaagcuuccu aaaucgguua     3480

ccagcagaua aacuaaaccu uaaauucagc augcaacaaa acgaaauguu cauccuugga     3540

cuacuagaag aagaauuuga uaaggcaaua aaagacaaug auaaaagccu acuuagcaaa     3600

uaccuguacu uaguaugguc aauugcagaa ggagauuacu gguuucgaca ucaucuggag     3660

accaaaaaua cagaauuaaa aaaaacaaau ggagcgaagg aaucaaaacg auacuuucga     3720

cuaucaacaa aaggacuaau agauuuaaac ccaguuaaag uaagauuaaa ucauuuaggc     3780

gagaucacaa agaucggaga auacuga                                         3807


<210>  5232
<211>  3810
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5232
augaacaacu ccauaaaauc aaaaccggaa guuacaauag gucucgaccu aggaguggga       60

agugucggau gggccauagu agauaaugaa acaaacauaa uucaucacuu aggaucacga      120

uuauuuucac aagccaaaac agcagaagac cgacggucau ucagaggagc aagaagacuc      180

auacguagac gcaaauacaa acuuaaacga uucauaaacc ucauauggaa auacaauucc      240

uacuucggau ucaaaaauaa agaagacauc cuuaacaacu accaagaaca acaaaaacug      300

cauaacacgg ucuugaacuu aaaacuagaa gcauuaaaug cuaaaauaga cccaaaagca      360

uuaagcugga uccuccauga cuaccugaaa aaccgagguc acuuuuacga agauaaccga      420

gacuuuaacg uguacccaac agaagaacua gcaaacuacu ucggggaaua cggauacuac      480

aaaggcauaa uagauagcaa agaagauaac gacgauaaac ucgaagaagg acuaaccaaa      540

uauaaauucu cuaaccaaca uugguuagaa gaaaucaaga aaguauuauc aaaccaaaca      600

ggucuaccag aaaaauucaa agaagaauac gaaucacucu ucucguacgu ccgaaauuac      660

ucagaaggac caggauccau aaauucaguc ucaccauaug gaauauauca ucuagacgaa      720

aaagaaggaa aaguagucca aaaauacaau aauauauggg acaaaacaau agguaaaugu      780

aguaucuuuc cggacgaaua ccgcgcgccc aaaaacucuc cgauagccau gauauucaac      840

gaaauaaacg aauuaucaac aaucagaucc uacaguauau accugacagg augguucauu      900

aaucaagaau uuaaaaaggc uuaccuaaac aaacuacuug acuuacucau aaaaacaaau      960

ucagaaaaac caauagaugc aaggcaauuc aagaaauuaa gagaagaaac gauagccgaa     1020

ucaauaggaa aagaaacuuu aaaagacgua gaaagagaag aaaaacuaga aaaagaagac     1080

cauaaaugga aacuaaaagg acuaaaacua aauacaaacg gaaaaaucca auacaacgau     1140

cucuccucau uagcaaaauu cguccauaaa cuaaaacaac accuaaaacu agacuucuua     1200

cucgaagauc aauauaccac auuagacaaa aucaauuucc uacaaucucu cuuuguguau     1260

cuuggaaaac acuuaagaua cagcaaccgc guagauagug ccaauuugaa agaguucuca     1320

gauucaaaca aacucuuuga acguguucua caagaacaaa aagaugaccu guuuaaacua     1380

uuugaacaaa cagacaaaga cgaugagaaa auauuggcac aaacacacuc acuuucuaca     1440

aaagcuaugu uacuagcaau cacaagaaug accaauuugg acaaugacga agacaaucag     1500

aaaaauaaug auaaaggcug gaacuucgag gccaucaaaa acuuugauca aaaauucauu     1560

gauaucacaa agaaaaauaa caaccugagu cuuaaacaag acaaacgaua uuuagaugau     1620

agauucauaa acgaugcuau ucuaagucca ggaguaaaac gaauccuuag agaagccacu     1680

aaaguauuca augcuauucu aaaacaauuc agcgaagaau augacgugac aaaaguagua     1740

auagaauugg cccgagaacu uagcgaagaa aaagaguuag aaaauacuaa aaauuacgaa     1800

aaauuaauaa agaaaaauuc agacaaaaua ucagagcgcc ucgaagcccu agacauagca     1860

gaagacaaaa ucgaagauau ccuuaaaucc ccaacaaaau ccuacaaagu ccucuugugg     1920

cuacaacaag aucauauuga uccauacuca caaaaagaaa uagcauucga agacauacuc     1980

acgaaaacag agaaaaccga aauugaucac auaauaccau acuccaucuc auuugacgau     2040

aguucaucaa acaaacuccu aguacuagca gagagcaauc aagcaaaaag uaaccaaacu     2100

ccauacgagu ucauaacaag cggaaacgca gguauaaaau gggaagauua ugaagcauac     2160

ugcaggaaau ucaaagacgg agacacgucu uuacuagauu ccacacaaag aaguaagaaa     2220

uucgcaaaaa ugaugaaaac agacacaucc uccaaauacg auauaggcuu ccuagcacgc     2280

aaccuaaaug auacccgaua ugccacaaua guauuuagag augcauugaa ggauuacgcc     2340

aauaaccacu uaguagaaga caaaccuaug uuuaaaguag ucuguaucaa cggaggaguu     2400

acuagcuucc uaagaaagaa cuucgacaaa agcugguacg ccaagaaaga ucgagacaaa     2460

aacauacacc acgccgucga ugcaagcaua aucagcauau ucucaaacga aacuaaaaca     2520

cuauucaauc aacucaccca auuugcugac uauaaauuau ucaaaaauac ugacggauca     2580

uggaagaaaa uagacccuaa aacgggaguc guaacugaag uaacagacga aaacuggaaa     2640

caaauccgcg uaagaaacca aguuagugaa auagccaaag uaauaaacaa augcauacaa     2700

gauagcaaua ucgaacguaa agcgagauau agcagaaaaa uagagaauaa aaccaacaua     2760

ucacuauuua augacacagu guauucugca aagaaaguag gcuaugaaga ccaaaucaaa     2820

aggaaaaauu uaaaaaccuu agacauacac gaaucagcug aagaaaacaa aaauuccaaa     2880

guaaagaaac aauucgucua uagaaaauua guaaauguau cacuacucaa caaugacaaa     2940

cuagccgacc uauucgcaga aaaagaagac auacuuaugu aucgagcaaa ccccugggua     3000

auaaauuuag cagaacaaau cuuuaaugaa uauaccgaaa auaagaaaau caaaucacaa     3060

aacguauuug aaaaauacau gcuugacuua acuaaagagu ucccagaaaa auucagugaa     3120

gcguucguua aaucaaugcu ccgaaacaaa accgcaauca ucuauaaugu cgaaaagaaa     3180

guaguacaca ggauaaaaag acucaaaauu uugucaagug aauugaaaga aaauaaacug     3240

aguaacguaa uaauaagaag uaaaaacgaa aguggcacaa aauuauccua ccaagacaca     3300

auaaacucaa ucgcucuaau gauaaugcga agcauagauc caacagccaa gaaacaauac     3360

auacgcguac ccuuaaacac uuuaaacuug cacuuaggag accaagauuu cgaucuacau     3420

aauauagaug cauaccugaa gaaaccuaaa uucguaaaau aucucaaagc gaacgaaauc     3480

ggagacgaau acaaacccug gagaguauua acaaguggga ccuuacuuau ucacaaaaag     3540

gacaagaaac uaauguacau auccaguuuc caaaaucuaa aagaugucau ugaaaucaag     3600

aaucuaauag aaacggaaua caaagagaac gacgauucag accccaagaa gaagaagaaa     3660

gccaauagau uccugaugac gcuaucaacg auccuuaacg auuacauauu gcuugaugca     3720

aaagauaacu uugauauucu aggcuuaagc aaaaaucgca uagacgaaau auuaaauuca     3780

aaacuuggau uagacaaaau aacuaaauga                                      3810


<210>  5233
<211>  3810
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5233
augaacaaua gcauaaaguc gaaacccgaa guuacaaucg gauuagacuu agguguagga       60

ucaguaggau gggcaauagu agauaaugaa acaaauauca uucaccaucu cggaucgagg      120

cuauuuucgc aagcaaaaac cgcagaagac agacgcaguu uucgaggugu cagaagauua      180

auucgacgcc gcaaauacaa auuaaaaaga uucgucaauu uaauauggaa auacaauuca      240

uauuuuggau ucaaaaauaa agaagauaua cucaacaacu accaagaaca gcaaaaacua      300

cacaauacug uauuaaauuu aaaaagugaa gcauuaaacg cuaaaauuga uccaaaagcu      360

cuauccugga uacuacauga uuaucugaaa aaccgcggac acuucuacga agacaauaga      420

gacuuuaacg uuuaucccac gaaagaacua gcaaaauauu ucgauaaaua uggguacuau      480

aaaggaauaa uugacucaaa agaagauaau gacaauaaac uagaagaaga acuaacaaag      540

uauaaguuua guaauaaaca cugguuagaa gaagucaaaa agguccuauc aaaucagaca      600

gggcuaccag aaaaauucaa agaagaguau gaaucauuau ucucauacgu acgaaacuac      660

agugaaggcc caggaucaau aaacucagua agcccuuacg gaaucuauca ucucgacgaa      720

aaagaaggua aaguaguaca aaaauacaac aacaucuggg acaaaacuau cggaaaaugc      780

aauauauucc cagacgagua cagagcacca aaaaauucac caauagcaau gauuuucaau      840

gagauaaacg aauuaaguac aauaagaucc uauaguaucu accuaacagg augguucauc      900

aaucaagagu uuaaaaaagc auaccuaaau aaacuacuag accuucucau uaaaacgaau      960

ggcgaaaaac ccauagacgc acgccaauuc aaaaaacuac gugaagaaac aaucgccgaa     1020

ucaauuggca aagaaacuuu aaaagacgua gaaaacgaag agaaacuuga aaaagaagau     1080

cacaaaugga aacucaaagg acuuaaacuc aacacaaaug guaaaauaca auacaacgac     1140

cuaagcagcc ucgccaaguu ugugcauaaa cugaaacagc aucugaaauu agacuuccua     1200

uuagaagacc aauacgcaac acuagacaaa auaaauuucc uccagagccu guucguauac     1260

cuaggcaaac auuuaagaua caauaacaga guugacagug cuaaccuaaa agaauuuuca     1320

gauaguaacc gccuauucga acgcgucuua caagaacaaa aagauggacu auucaaacuu     1380

uuugaacaaa cagauaaaga ugaugaaaaa auacuagcuc aaacacauuc acugucaaca     1440

aaggcaaugu uacuagcgau aacacgaaug acaaaccuag acaacgacga agacaaucaa     1500

aaaaacaaug acaagggcug gaacuuugaa gcaauaaaaa acuucgauca aaaauucaua     1560

gauauaacua aaaccaacaa caaucuauca cucaaacaag auaaaaggua ucuagaugac     1620

agauucauua augacgccau acucucacca gguguaaaac ggauccucag agaagccaca     1680

aaaguuuuua acgcaauacu aaaacaauuc ucacaagagu acgaugucac caaagucguu     1740

auugagcucg cacgagaauu gucagaagaa aaggaacuag aaaauaacaa aaacuauaag     1800

aaacucauca aaaaaaacuc cgacaaaaua agugaagggc ucaaagcgcu cgacauugca     1860

gaagauaaaa uagaagacau auuaaaaagc ccaacgaaau cguauaaagu acuauuaugg     1920

cuccaacaag accacauaga uccauacagc caaaaagaga uagcauucga agacauucuu     1980

accaaaacag aaaaaacaga gauagaccac auaauucccu acucaauuag cuuugacgac     2040

ucaaguucca auaaacuacu aguucuagcc gaauccaauc aagcaaaguc uaaccaaaca     2100

ccauaugaau uuauuacauc cggaaacgca ggaauaaaau gggaagauua cgaagccuau     2160

ugcagaaagu ucaaagacgg agacacaucc cuuuuagacu ccacacaaag aagcaaaaaa     2220

uucgcuaaaa ugaugaaaac agauacuucc ucuaaguaug auauuggauu ccucgcaagg     2280

aacuuaaaug auacuagaua cgcuacgaua guauucagag augcacuaaa agacuaugcu     2340

aauaaccacc uaguagaaga uaaaccaaug uucaagguag uauguaucaa cgguggugua     2400

acaagcuucu ugagaaaaaa uuuugacaaa ucaugguaug caaaaaaaga ucgagacaaa     2460

aacauccauc acgcagucga cgcaucaauc auaaguauau ucaguaacaa aacaaaaacu     2520

cuauucgauc aacuaacaca auucgcugac uacaaacuau uuaaaaauac agauggauca     2580

uggaaaaaaa uugacccgaa aaccggagua guaaccgagg uaaccgacga aaacuggaaa     2640

cagaucagag ucaggaauca agucaguaaa auugcagaag agauugauaa augcauucaa     2700

gacagcaaca ucgagagaaa ggcuagauac uccagaaaaa uagaaaacaa aacaaacaua     2760

aguuuauuca acgacacugu guacuccgcc aaaaaaguug gauacgacga ucaaauaaaa     2820

aggaagaacu uaaaaacauu agauauagac gaaucaguug aagaaaauaa gaauaguaaa     2880

guaaaaaaac aauuuguaua ccguaagcua guaaacguaa gcuuacuaaa uaacgauaaa     2940

cucgcagacc ucuucgcaga aaaagaagac auacuuaugu auagagcaaa uccaugggua     3000

auaaaucuag cagaacaaau auucaacgaa uacacagaaa auagaaaaau aaaaucgcag     3060

aacguauucg gaaaauauau guuggaccuc accaaagaau uuccggaaaa auuuagugaa     3120

gcguuuguaa aaucgaugcu acgaaacaaa acagccauaa uauacaacgu agaaaaaaaa     3180

gucguucaca gaaucaaacg ucuaaaaauu cuaucaucag aacuuaaaga aaacaaacua     3240

uccaauguaa ucauaagaag caaaaacgaa agcggcacaa aacuaucgua ccaagauaca     3300

aucaacagcg uugcccuaau gaucaugcga aguauagauc caaccgcuaa aaagcaauac     3360

auacgaguac ccuuaaacac acucaaucuu caccucggcg accaugacuu ugaccuacac     3420

aauaucgaug cauaccuaaa aaagccaaag uucgucaagu accuaaaagc caaugaaaua     3480

ggcgacgaau acaaacccug gagaguccuc auaaguggcu cacuauuaau acacaaaaga     3540

gacaagaaac ucauguauau aucaucguuc caaaaccuaa acgaucuaau agaaauaaaa     3600

aaucucauag agaccgaaua caaagaaaau guagacucug auccaaaaaa aaaaaagaaa     3660

gcaagccaaa uccuuagaag uuuaucuaca auauuaaacg acuauauacu cuuagacgca     3720

aaagacaacu uugacauuuu aggucuuucu aagaauagaa uugacgaaau acuaaacagc     3780

aaacuagauc ucgacaaaau cgcaaaauga                                      3810


<210>  5234
<211>  3822
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5234
augaagaaaa uccucgguuu ggaucuagga acuaauucca uaggaugggc acuuauagaa       60

guagaugaaa acaauuuucc cauacgcaua auagcaaugg ggucgcgaau uauacccuua      120

agcaguaaug acaaagacga auuucaaaga ggacuaucaa uaacuaaaaa ccaaaguaga      180

acaguaucac ggacacaaag gaaaggauau gacaggaaac aacuaaagaa aagugaugac      240

uuuaaauaca gucucagacg aauacugaau aaauacaaca ucuuuccguc agaagaacua      300

cuaaaacuuc caaugcucga uuuauggaaa cuuagguccg augcgguaaa cccgaaugaa      360

aacauaucug cagaacaauu aggucgaaua cuauacaugu uaaaucagaa aagaggguau      420

aaaagugcua gaucagaggc caaugcagau aagaaagaca cagacuacgu acuagcaguu      480

aaagguagau augcucaauu aaaagacaga aaucaaacca uaggacaaua cuuuuacgaa      540

gaauuacaua acgccaaaac cacuaauuca uacuucagga caaaagaaga aguuuaucca      600

agagaagccu acauagaaga guucgacaca auaaucaaug cacaaaaauc aaaacacgac      660

uuucugacug augaaguagu acaccaauua agaaacgaaa ucauauacuu ccaaagaaaa      720

cugaaaucac aaaaaggacu uguaucaauu ugcgaauuug aaggauucga gaaaacaauc      780

uucgacaaag aaaaagacaa aaacaaagua guauuugugg gacccaaagu ggcaccgaaa      840

acaagccccu uguuucaacu augcagaaua ugggaagucg uaaacacaau cacucuaaaa      900

aucaaaaacc ccgaaggcuc caaauacaaa uggggagaga aaauaccaaa uauaaaagaa      960

aaagaagcac uagcggacua uuuauucaag aaugaaacac uaucauuuaa caaacuucuc     1020

gauauacuug aauuaaagaa agaaaacgua uacguuaaca aacaaauaau aaaaggcaua     1080

aaaggaaaug aaacauacag ugcacuccau agaauacugg gagacaacga auuauuaaaa     1140

uucgauguaa gcauuauacc gagcaaccau acaucuaugu uaguugacaa aaagaccggu     1200

gaaauacuag aagaacgagc agggcuagaa cuagacgucu cuuuagaaaa agaaccccua     1260

uaccaauuau ggcacacuau cuauuccaua aaagaucugg augaaugcaa aaaugcgcuc     1320

auuaaaagau ucggauucga cgacgaaaua gcagaaaaac uaucacaccu agauuucaac     1380

aaacaagccu ucggaaauaa aucaaacaag ucaaugcgaa aaauacugcc uuuucuuaug     1440

gaaggauaua auuacagcca aucaugcaca cuggcaggau auaaccauuc aaacucacua     1500

acuaaagaug aaagagaaca acaaguaacc uuagacaaac uugaacuacu accaaagaac     1560

agccuaagac agcccaucgu agagaaaaua cuaaaccaaa ugauaaaugu ggugaacgca     1620

auacuagaag aauacggcaa acccucagaa auaagaaucg aacuugcccg agaauuaaaa     1680

caguccaaag acgaacgaca cgacgcagac cuucaaaaca guaaaaauaa gaaauugaac     1740

gaagaaauag gcaaaaggcu gacagaacuc ggacugccag ucaccaaaag auacauacaa     1800

aaauacaaau uuauauuccc aagccucagu aaaucucuua aagaugcuca agucaauaac     1860

caauguauau acugugguga aaccuuuaac cuaacugaag cguuauccgg agaugcauau     1920

gauguugacc auauaguccc gaaaagccuc uuauucgacg acagucagac gaacaaagua     1980

cuggugcaca ggaaaugcaa uucuacgaag acaaaucaaa cggccuacga cuacauagca     2040

ucaaaaggug aagaagaagu acaaauauac ucagccagag ucgacgauug guucaaaaga     2100

ggaauaauaa guuauucgaa aaugcaaaga uuaaaaguaa gucacaaaga auaccucgaa     2160

agaaaauccu uaaacaaaga gacugaaaca gacaagaaau uaugggaaaa cuucauagac     2220

cgucaauuaa gagauacgca guacaucagu cguaaaagua aagaaauauu gcaaaaaguu     2280

uguaacaacg uaaccaccac cgaaggagga guaacggcua aacuaagaga cuuauggggu     2340

ugggaugaca uauuaaugaa uuuacaaaug ccaaaauaua aagaacuaag acaaaccguu     2400

auaaaagaau ggaccaguga acacggcaaa cgaaaacacc aaaaagaaga gauugaaaau     2460

uggacaaaaa gggaugauca uaggcaucau gccauagacg cgcuuguaau ugcauguacu     2520

aaacaaggcu ucauacagag aaucaauaca cuuaacagua gcgagaccaa agaccuaaug     2580

caacaagaaa uuaacgaagc aaagaaacua cuaggagaaa auuauaaaga caaagaauuu     2640

gaaaacucaa acgaauuagu caaaaaucac aaaggaaggc ucucaaagcu agacgacuau     2700

cuaagacuac aaaaaccaau aaaauuuaac accaaauacg ucgaaaaaga agcagacaaa     2760

auucuaauau cauucaaagc aggaaagaaa guugccacca uaaccaaauu caaagcaacu     2820

gggaaaaaua aagacacagg agucauaguu ccccgaggag cucuacacga agcaggacuc     2880

uacggaguaa gaacaauaua uagcaaaggc ggaauagcag aaaaacauau uauuaaaaag     2940

uacaaagugg gagucagugc acaagaucac auauucacug gaaaagaaag uuacagcgua     3000

aagaaagaua aaaacggaaa cgaaaaaguu gacgauaaaa uaaaaguagu ccuagacagu     3060

auaguagaca guaaagucaa agaaaucguc uuaaacagac uaaauuucgg cuuuucagac     3120

ggagaagacu acaaaaacaa cguaaagaaa gcacucaaca auuucaaaaa ccuagacgaa     3180

uauccucuau gguacaacga aaacaaagga auaaaaauuc uauccaucaa acuaaagaca     3240

ggauuaaaag auuccguagu accauuaaga uuaaacgaaa guaaugaagu aauaacauua     3300

guaaaaacag guaacaacca ccacauagca auuuacgaag auaaaaacuc caaaccuguc     3360

caacacucau gcacauucug gcaugcagua gaaagaaaga aauacaacau acccgcugua     3420

aucaagaaau caagcgaugu auggaaccaa cuccuagaca aagaacuacc ugaaucuuuu     3480

cuaaacaacu uaccagaaga caauuugaau uuaaaauuca guaugcaaca aaacgaaaug     3540

uuuguacuag gguuaucaca agaagaguuc aaagaagcca uagaaaauaa ugacaaauca     3600

uuauuaucca aauaccugua ucucguaugg ucuguuagcg aaaauaauua uugguuuaga     3660

caccauuuag aaaccaaaaa uucugauuua aagaaaacag aaggggcaaa agaaaguaaa     3720

cgacuauaca acauuagguc aguuucauca uuguucaguc uaaacccagu uaaaguuagg     3780

uuaaaccacc ugggagaaau aacaaaaauu ggggaauauu ga                        3822


<210>  5235
<211>  3825
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5235
augacgaaac acauuuuagg cuuagaccua ggaacaaauu cgaucggaug ggccaucaua       60

caaguagaua acaacaacaa uguccccaua caaauuauag caaugggcag ccgaauuaua      120

ccuuuagacu ccaacgacag agaccaauuc caaaaaggac aagcaauauc aaaaaauaaa      180

gacagaacaa ccgcacggac acaaagaaaa ggauaugaca gaaaacaacu uaaaaaauca      240

gacgacuuca aauacucacu uaaaaaaauc uuagagaaac ucgacaucuu uccaacagaa      300

gaauuaauga aacuuccaac ccuagaccuc uggaaacucc gaucagacgc ugucucgaac      360

auagaagaua uaacacccaa acaauuagga agaauccuuu acauguuaaa ccaaaaaaga      420

ggauacaaga gugcgcgcuc agaggcaaau gcugauaaaa aggauaccga uuacgucgca      480

gaaguaaaag gaagguauac acaacuaaaa gacaaaggcc aaacgcuugg ucaauacuuc      540

uacaaagaac ucagcgacgc uaaccaaaac aauacguauu auagaguaaa agagaaagua      600

uacccaagag aagcauauau cgaggaauuc gacgcuauua ucaacguaca aaaaaguaaa      660

cacagcuucc uaacagauga aguaauccac ucauuacgaa acgagauaau uuacuaucaa      720

cggaaacuca aaucgcagaa aggucucguu uccauaugug aguucgaagg auuugaaacu      780

acguacuuug acaaaaaaac acaacaagau aaaacaaucu ucaccggccc caaaguugcu      840

ccaagaacaa gcccacucuu ccaguuuugu aaaauauggg aaguugucaa caauauaagu      900

uuaaaaacga aaaauccaga agguucaaaa uacaaaugga gcgacagaau accaaccaua      960

gaagaaaaac agacaauugc caacuacuua caagaaaaug aaaaucugag uuucauugaa     1020

cucuuaaaaa uucugcagcu caaaaaagaa caaguuuacg ccaauaagca aauacugaaa     1080

ggaauacaag gaaacacaac cuuuagcgcc auacacaaaa ucauaggaaa cagugaacac     1140

uuaaaauuug auaucgaaac aauaccauca aagcacuuug caguccuugu ugacaaaaaa     1200

accggggaaa uuuuagacga gagagacagu uuagagcuca auucagcacu ggaacaggaa     1260

ccauucuauc aacuuuggca uacaaucuac ucuauaaaag accuagacga auguaaaaaa     1320

gcccuaauaa aaagauuuaa cuucgaagaa gagauagcug agaaguuaag uaagauugau     1380

uucaauaagc aagcuuuugg caauaaaucu aauaaagcca ugagaaaaau gcuaccauau     1440

uuaaugcuag gcuauaacca aucagaagcu gaaagcuuug cagguuacaa ccgucgacua     1500

acuaaagaag aaaaauccaa aaacguaucg gaugaaccac uacaacuucu cgccaaaaac     1560

uccuuaagac aaccaguggu ugaaaagauu cuaaaccaga ugauaaacgu agucaacgcg     1620

aucauugaaa aauauggcaa accagaagaa auuagaguag aacuagcacg agaacuuaaa     1680

caauccaaag acgagcgcga agaugcagau aaacaaaacg guuuuaacaa aaaacuaaau     1740

gaauuaguag cuacaaaacu aacggaauua gguuuaccaa caacuaaaca uuacauccag     1800

aaauacaaau ucauauuucc agcgaaagac aaaaauugga aagaggcaca ggucgccaau     1860

caaugcauau acugcggcga uacauucaac cuuacagaag cacucucagg agacaacuuc     1920

gauguagacc acaucguacc aaaagcacuu cuauucgacg acagccaagc gaacaaaguc     1980

uuaguucacc gaagcuguaa cagcacaaag accaacaaca cagcauauga cuacauaaca     2040

aaaaaaggca gccaagccuu gaacgauuau guagcuaggg uggacgacug guuuaaacgg     2100

gguauuauaa guuacgggaa aaugcaaaga uuaaaaguau cauuugaaga auaccaagaa     2160

cguaagaaaa ucgggaaaga aacagaagcu gauaaacgga uaugggaaaa cuucaucgac     2220

agacaacuaa gagaaaccgc auauauagcu aaaaaagcga aagaaauacu ggaaaaagua     2280

ugucauaacg uuacaucuac agaggguaac gucacagcaa aauugagaca acuaugggga     2340

ugggacaacg uucuaaugaa ccuccaauua ccaaaauaua aggaauuaga gaaaaaaaca     2400

aaacaaacau uuacgcagcu aaaagaaugg acuucagacc acgggaacag gaaacaccaa     2460

aaagaagaaa ucauaaauug gacaaaaaga gaugaucaca gacaccacgc uauagacgcc     2520

uuaguaauag cuugcacgca acaggguuuu auacaaagaa uaaacacccu uaguuccuca     2580

gacgucaaag augaaaugaa aaaagaacua gaagaagaca aaacaguuua uaaugaacgu     2640

cugacucucc uugaaaauua cuuacuagaa aaaaaaccau uuucuacaga agagaucgaa     2700

aaagaggcag acaaaauacu cgugaguuuu aaagcaggaa aaaaaguagc aaccuuauca     2760

aaauauaaag caacagguau aaacgaaauc aaaggaguac uaguuccaag aggaccacuu     2820

cacgaacaaa gcguuuacgg aaaaauaaaa guaauugaaa aggacaaacc uuuaaaauac     2880

cuauuugaaa acagugauaa gauaguaaac ccauuaauaa aacaccuagu aaaaacacga     2940

uugcuggaaa augaaaauaa ugcccaagca gcauuaguaa cauuaaaaaa uaaaccaaua     3000

cuauuaaaua acaaacaaac agaaauccuc gaaaaagcca guugcuacaa ugaagcgacu     3060

guacucaaau auaaacucca gagccuaaaa gcuagucaaa uagaugauau aguggacgaa     3120

aagauaaaau uuuuaauaaa agaaagacua agcaaauucg gaaauaagga aaaagaagca     3180

uuuaaagaua uacucugguu caaugaaaaa aaacaaauac cuauuacauc aauaagacua     3240

uuugcacggc cugacgccaa uaaucuccaa gucauaaaaa aacacgaaaa agguaaaaac     3300

auagguuucg ucuuaagcgg uaacaaucau cauauugcca uauacgaaga uaaaaauaac     3360

aaauuaauac aacauaucug ugacuuuugg cacgcagucg aaagaaaaag aaacaacaua     3420

ccaguuuuaa uagaagacac aaguacaaua uggaaucacc uaauaaacga agauuuuagu     3480

gaaaguuuuc uaaacaaauu accuaacgac ucacuaaaac uuaaauucuc uuuacaacaa     3540

aacgaaaugu ucauauuagg acuaccuaaa gaacaauccg aagaggcuau uaaaucaaau     3600

aacaaaagcc uccuaucaaa gcaccuauau uuagucugga guauuaccga uggugacuau     3660

uucuuuagac aucaucuuga aacaaagaac acagaauuaa aaaaaauaga uggcucaaaa     3720

gaaucgaaac gauaucuuag acuaucgaca aaauccuuag ucgaccuaaa uccaauaaaa     3780

guacggcuaa accauuuagg agaaauaaca aaaauuggag aauga                     3825


<210>  5236
<211>  3846
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5236
auggaagaug acagccagca ugauauuaga gaaauauacu uagcaauaca ucauauggua       60

aaauauagag gaaauuuucu aguugaagga acacuagaaa guaguaaugc guuuaaagaa      120

gaugaauuac uuaaacuccu agggaggaua acacguuacg aaaugagcga aggagaacaa      180

aacagugaca uagaacaaga ugacgaaaau aaacugguug cuccugcaaa ugggcaacuc      240

gcagacgccc uaugugcaac aagaggcagu aggagcauga gaguagacaa ugcauuagag      300

gcuuuaucgg cgguaaauga ucuaucacga gaacaaagag cgauaguaaa agcuauuuuc      360

gcuggucugg aagggaauaa auuagaccua gcaaaaauau uuguaaguaa agaauuuucg      420

agcgaaaaua agaaaauacu aggaauauau uuuaacaaaa gcgauuauga ggaaaaaugu      480

guacaaauag uagacucugg acuguuagau gaugaagaaa gagaguuccu agauagaaug      540

caaggacaau acaacgccau cgcauuaaaa cagcuauuag gaagaagcac cucggucagu      600

gacaguaaau gugcuaguua ugaugcacau agggcaaacu ggaauuuaau aaaacuucaa      660

uuaaggacaa aagaaaacga aaaagauauu aaugaaaauu auggaauauu aguaggaugg      720

aaaauugaua guggccaacg gaaaucagua aggggugaga gugcuuacga aaauaugaga      780

aagaaagcga auguauuuuu caagaaaaug auugaaacga gcgaccucuc agaaacugau      840

aaaaacaggu uaauacacga uauagaagaa gacaaacuau ucccuaucca gagggacagc      900

gauaacgggg uuauacccca ccagcuacau caaaaugagc ucaaacaaau aauuaagaaa      960

caaggaaaau auuauccuuu ccuauuagau gcauucgaaa aagaugggaa acaaaucaac     1020

aaaauagaag gacuccuugc auuccgagua ccguauuuug ucggaccgcu agucguucca     1080

gaagacuuac aaaaaucaga uaauucagaa aaucauugga ugguuagaaa gaagaaagga     1140

gaaauuaccc cguggaauuu ugaugaaaug gucgauaaag augcuuccgg ucgcaaauuu     1200

auugaaagau uagugggaac ggauucguau uuacucggag aaccgacgcu accuaaaaac     1260

agucuacugu accaagaaua ugagguauua aaugaacuaa auaaugucag acuaagugua     1320

aggacuggca aucauuggaa cgauaaaaga agaaugaggu ugggaaggga agagaaaacg     1380

cuauuauguc aaagguuauu uaugaaagga cagacaguua caaaaagaac agcagaaaac     1440

uugcuaagaa aagaguaugg ccguacuuac gaacuaucag gguuaucuga ugagucaaaa     1500

uuuacgucua gucugucgac auauggaaaa augugcagaa uauuugggga gaaauauguu     1560

aacgaacaca gggaucuaau ggagaaaaua guagaauugc aaacaguuuu cgaagacaaa     1620

gaaacguuac uccaccaacu ucgacaacua gaagguauaa gugaagcaga cugcgcacua     1680

uugguaaaua cucauuauac agguuggggu agacugagca gaaaauuauu aacuacuaaa     1740

gcuggugaau gcaaaauuag ugaugauuuu gccccacgaa aacauucaau aauagaaauc     1800

augagagcug aagauagaaa uuuaauggag aucauaacug auaaacaauu ggguuuuucu     1860

gacuggauag aacaagaaaa uuuaggugcu gaaaauggaa guucacugau ggaaguggua     1920

gaugaccuaa gaguaucucc aaaagugaaa cgaggaauaa uacaaaguau aagacuaauu     1980

gaugauauaa gcaaagcagu aggaaaaaga ccuucacgua ucuucuuaga auuagcugac     2040

gauauacagc ccucaggcag gaccauauca aggaaaagca gauuacaaga ccuauaucgg     2100

aaugcaaacu uaggaaaaga auuuaaaggg auagcugacg aguuaaacgc auguagugau     2160

aaagaucuac aagaugacag acuauuccua uauuacacuc aauuaggaaa agauauguau     2220

acuggagagg agcuagauuu agauagacuc ucaagcgcuu augauauaga ccacauaaua     2280

ccacaagcug uuacccaaaa cgauaguaua gauaaucgcg uuuuaguggc gcgggcagaa     2340

aaugcaagga aaacugauuc auuuacguau augccucaaa uagcagauag aaugcgcaau     2400

uucuggcaaa uauuauuaga uaacggauua auuagcagag uaaaguucga aagguuaaca     2460

cgucaaaaug aauuuucaga gagagaaaaa gaaagguuug uacaacgauc auuaguagaa     2520

acacgacaaa ucaugaaaaa uguagcaaca cuaaugcgac agagguaugg uaauagcgca     2580

gcgguuauag gauugaacgc ugaacuuaca aaagaaaugc aucgauacuu agguuucuca     2640

cacaaaaaua gggauauaaa ugacuaucau caugcacaag augcgcuuug cguaggaauc     2700

gcaggacaau uugcggcuaa uagaggauuc uuugcagaug gcgaaguauc agauggugcg     2760

caaaauucgu auaaucaaua ucucagagau uauuuacgag guuauagaga aaaacugagu     2820

gcggaggaua ggaaacaagg gagagccuuu ggauuuaucg ugggauccau gagaagucaa     2880

gacgagcaga aaagaguaaa cccaagaacc ggagaagucg uauggagcga ggaagacaag     2940

gauuauuuaa gaaaagugau gaauuaccga aaaaugcuag ucacucagaa gguuggugau     3000

gacuuuggug cacuauauga ugaaacacgg uacgcagcaa cggaucccaa agguauaaaa     3060

ggaauaccgu uugacggugc aaaacaagau acaaguuuau auggaggauu cagcagugcg     3120

aaaccggcau acgcuguuuu aauagagagu aaaggaaaaa cuaggcuagu aaacguaaca     3180

augcaagaau auagucuacu aggagacaga ccgagugaug acgaauuaag aaaagugcua     3240

gcuaaaaaga aaagcgaaua ugccaaagca aacauucuau uaagacacgu accaaaaaug     3300

caacugauua gauauggagg gggauuaaug guaauaaaaa gcgccgggga gcuaaauaac     3360

gcacaacaac uaugguugcc auaugaggag uacugcuacu uugaugauuu aagccagggc     3420

aagggcucuc uggaaaagga cgacuugaag aaauuauuag auaguauauu gggaucgguu     3480

caaugccucu aucccuggca uagauuuacg gaagaagaac uagcagacuu gcacguugca     3540

uuugauaaau uaccggaaga ugaaaagaaa aaugucauaa caggaauagu aagugcauug     3600

caugccgaug caaaaacagc aaaucuaagu auaguaggca ugacaggaag uuggaggcgg     3660

augaacaaca aaaguggaua uacauucagu gaugaagaug aauuuauauu ccaaucaccg     3720

agcggauuau uugaaaaacg agugacagua ggagaacuua aacgaaaagc aaagaaagaa     3780

gucaauucca aauacagaac aaacgaaaaa cgauuaccua cauuaagcgg ugcaucgcaa     3840

ccauga                                                                3846


<210>  5237
<211>  3861
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5237
augaccaaaa auauuuuagg uuuagacuua ggaacaaaua gcauaggcug ggcauuaauu       60

gaaguugaug aaaauaauac accaauacga aucaucgcaa ugggaagccg aauaauacca      120

cuaaguacua gcgacaaaga agaauuucaa agaggacuau cuauaaccaa aaaccaaucu      180

cgcacagucu cucggacaca acgaaaagga uaugacagaa aacaacuuaa aaagagcgac      240

uuaaagaaaa uauugaacaa auauaacauc uucccaucag aggaacuucu uaaacuacca      300

augcuagauc uauggaaauu gaggucagau ucagcuaacc caaacgaaaa uauaagcgcc      360

gaacaacuag gaagaauccu uuauaugcuc aaccagaaaa gaggcuacaa aucagcaaga      420

ucagaagcaa augccgacaa gaaagauaca gacuaugucc aagcaguaaa aggaagauau      480

gcacaauuaa aagagagaaa ccaaaccaua ggacaauacu ucuacgaaga auuauacaac      540

gcaaacacaa auaauucuua cuuuagaauu aaagaagaag uauacccaag agaagccuac      600

accgaagaau uugauacgau cauuaaugcc cagaaaaaca aacacgauuu cuuaacagau      660

gaagugguac aucaacucag aaaugaaaua auauacuucc aacgaaaacu caaaagucaa      720

aaagguuuag uaucaauaug cgaguucgaa gguuuugaga aaacaguauu ugauaaagag      780

aaaaauaaag acaagguugu auuuguggga ucaaaaguug caccaaagac aaguccacua      840

uuucaacuau gcagaauaug ggaagucguc aauaccauuu cguuaaaaau caaaaauccu      900

gaagggucaa aauacaaaug gggagagaaa acgccaaacc uaaaggaaaa agaaauacua      960

gcugauuauu uauucaaaaa ugacucacua ucauucacga aauuacucga aauccucgac     1020

cugaacaaaa augacguaua uguaaauaag caaauacuaa aaggcauaaa aggcaaugaa     1080

acuuuuagug cucugcauac aguauuggga gauaaugaau uacuaaauuu cgauguuuca     1140

auaauaccau ccaaucacac cucaaugcuc guagacaaga aaaccggaga aauuuuggaa     1200

gaacgagccg gauuagaauu agacgcaucg cuagaaaaag aaccacucua ucaauuaugg     1260

cacacaauuu auagccuaaa aguucuagau gaaugcaaaa acgcacugau aaaaagauuu     1320

ggcuucgaug acgaaacggc cgaaaaacua ucucauauag acuucaauaa acaagcauuc     1380

ggaaacaaga guaauaaauc uaugaggaaa auacuaccau uucugaugga aggauaugau     1440

uauucgcaaa guugcucacu ugcaggauau aaccacucaa auagccuaac aaaagacgaa     1500

cgagaacaac aaauaacacu agaccgacug gaacuacuuu ccaaaaacag uuugagacag     1560

ccaauaguag aaaagauauu gaaucaaaug auuaacguag ugaacgcuau ccuagaacaa     1620

uauggcaaac uaucugaaau aaggguugaa cucgcacgag aacuaaagca aucaaaagau     1680

gaaaggaaug augcagaucu acaaaauucu aaaaacaaga aacucaauga agaaauugga     1740

aaacgcuuag ccgaacuagg gcuaccagua accaaaagau auauacaaaa auacaaauuu     1800

auauucccgu cauuaagcaa aaacuuaaaa gaugcccacg uuaauaauca auguauuuac     1860

uguggcgaaa ccuucaacuu aacugaagca cucucgggug augcuuucga cguagaccac     1920

auagucccua aagcacugcu auuugacgau ucucaaacaa acaagguacu uguacaucga     1980

aaguguaacu cgacuaaaac uaaucagacc gcauacgacu auauagggac uaaaggagaa     2040

gaacaaguuc gaauauacag cgaaagagua gacgauuggu uuaagagagg cauaauaagu     2100

uauucaaaaa ugcagcgauu aaaaguuuca cauaaagaau aucuagaaag aaagaaauua     2160

ggaaaagaaa cagaaaccga caagaaauua ugggaaaauu uuauagaucg acaauugcga     2220

gauacucaau acauaaguag aaaagcacga gaaauuuugc aaaaaguuug caacaacgua     2280

acaaccacag aaggcgguau cacagcaaaa cuaagaaacu uauggggaug ggaugacguc     2340

cuuaugaauc uacaaaugcc aaaauacaaa gaacuuggac aaacagucau caaagagugg     2400

accucagaac acgguaaaag aaaacaccaa aaagaagaaa uagagaacug gacaaaacga     2460

gacgaucaua gacaccaugc uaucgaugcc cuaguagucg cuuguacaaa acaaggauuc     2520

auacaaagga ucaauacuuu aaacuccuca gauaccaaag aucuuaugca aagagaaauc     2580

aaagaagcaa agaaacuucu aggugaaaac uacaacgaga aagaauuuga aaacgacaau     2640

gaauuuguua aaaaccauaa agaaagacug ucccaauuag augacuacuu aagguuacaa     2700

aaacccguaa aauuugacac aaaauaugua gaacaagaag cagauaaagu auuagugagc     2760

uucaaagcug guaagaaagu agcgacuaua acaaaauuua aagcaacggg uaaaaacaaa     2820

gacaccggag uaauaguuuu acgaggagcu cuacacgaac agucagugua cggaaaaauu     2880

aaaguaaucg aaaaagauaa accguuaaaa uaucuuuuug aaaauuccaa gaaaauagua     2940

aaccccaaaa uaaaaguuuu aguggaggaa cguuuagcag aaaaugaaaa caauuccaaa     3000

uuaaguuuau ccacccugaa gaaaaaccca auauuccuua acgaggagaa aaccgaaaua     3060

cuggaaaaag ccuccugcua caacgaugcc acaguccuga aauacaaacu ccagucauug     3120

aaagcaucac aagcggauga uauaguagau gacagaguga aauuccuagu aaaagaaaga     3180

uuggcacaau uuggaaacaa agaaaaagaa gcauucaaag acacccuuug guuaaacgaa     3240

gaaaaacaaa uaccaauuau aacaguacga uuauuugcua gaccagacgc aaacaauuua     3300

caagccauua agaaagauaa aaauggcaga gacauaggau uuguacuaac cgggaauaac     3360

caccacauag caauauacga agacaaggac aauaaauuag uacagcauag cugcacauuu     3420

uggcaugcug ucgaacgcaa gaaaaacaac guaccagccg uaaucaaaaa cucuagugau     3480

guauggaacc agcuauugga caaagaauua ccagaaucau uuuugaacaa acuaccaaua     3540

gacaaacuua aacucaaauu cucgcuucaa caaaacgaaa uguucguauu gggauugcua     3600

caagaagauu uugaagcagc cauagaaaau aaugauaaaa ccuuacucag uaaacauuua     3660

uaccuaguuu ggagcauaac cgacggcgac uauuucuuua gacaccaccu agaaacuaaa     3720

aauacagaac uuaagaaaac ugaaggagca aaagaaucca aaagauacuu ucgauuaucc     3780

acaaaaggac uaaucgaccu aaauccagua aaaguaagac uuaaccaccu aggagaaauc     3840

acaaaaauag gugaauauug a                                               3861


<210>  5238
<211>  3864
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5238
augaaaacag uccuaggauu ggauuuaggu acaaacucua uaggcugggc auuaauagaa       60

uuagacgaaa auaacauacc uauucgaauu guagcaaugg gcucacgaau uauaccacuu      120

ucaacuucag acaaagaaga guuccaaaga ggacuuagua uaaccaaaaa ccaaucccga      180

acgguaagcc gaacacaacg aaaaggcuac gacagaaaac aacuaaagaa auccuacuua      240

aagaaaauac uaaacaaaua caauauauuc ccguccgaug aauugcuaaa acucccgaug      300

uuagacuuau ggaaacuuag aagcgauagc gcaaacccaa augaaaacau cagugccgaa      360

caacuaggca gaauccucua cauguuaaac caaaaacgag gcuauaaauc ggccagaagc      420

gaagccaacg ccgauaagaa agacacagac uauauccaag cagucaaagg gcgauacgcc      480

caauuaaaag auagaaauca aaccauugga caauacuuuu acggagaauu aaaugacgcu      540

aacgaaaaca auucuuauuu ccgaauaaaa gaagaagucu auccuaggga agcauauaca      600

gaagaguucg acacgaucau aaacgcacag aaagacaaac augacuuuuu aaccgaugaa      660

guuauacacc aacugcgaaa cgaaauaauc uacuuccaac ggaaauuaaa aagccaaaaa      720

ggccuaguau caaucugcga auuugaagga uucgagaaaa cuguauucga caaagagaaa      780

aacaaagaca aagucguuuu ugucggacca aaaguagcac cuaaaaccuc cccauuauuu      840

caguuaugua gaaucuggga aguaguuaau accauaacac uaaaaaucaa aaacccugaa      900

ggaagcaaau auaaaugggg ugagaaaaua ccagacguca aagaaaaaga aauuuuagca      960

gacuaccucu ucaaaaauga aagucuaucu uuuacaaaau uacuagaaau ccucaaacuc     1020

aauaaaaaua acguuuacuu uaauaaacag auacuuaaag gaauaaaagg uaacgaaaca     1080

uauagcauau ugcacuccau acuuggggau ucugaacuac uaaauuucga cguuagcaua     1140

auaccaucaa aucauacauc uauguuaauc gacaagaaaa caggcgaaau cuuagaagaa     1200

cgugcgggau uagaacuuga uacaucucuu gaaaaagaac cuuuaaauca auuauggcac     1260

acaauuuacu caauaaaaga ucucgacgaa ugcaaaucag cgcuaauaaa acguuuugga     1320

uucgaugacg aaaccgcuga caaauugagu cgcauagauu uuaauaaaca agcguuugga     1380

aacaaaagua auaaaucaau gagaaaaaua uuaccauuuc uaauggaagg cuaugauuac     1440

ucacaaucuu guagccuagc cgguuauaac cacuccaaua gcuuuaccaa agaugaacga     1500

gaacaacaaa uaacauuaga uaaauuagaa cuacucccca aaaacucauu acgccaacca     1560

aucguagaga aaauuuuaaa ccagaugaua aaugucguaa acgcuauccu cgaaagauac     1620

gguaaaccaa gcgaaauaag aguagaacua gcuagagaac uaaaacaauc uaaagaugaa     1680

cgcaacgaca cugaucuuca aaauucuaaa aauaagaaac uaaacgaaga aauaggcaaa     1740

agacucgcgg aacuuggacu accacuaacc aaaagauaua uacaaaaaua caaauuuaua     1800

uuuccauccu uaagcaaaaa ccuaaaagac gcacauguaa acaaccaaug cauauauugc     1860

ggagaaacau uuaaccuaac cgaagcacuu ucgggagaug cauuugaugu ugaucacauu     1920

guuccgaaag cauuacuauu cgacgauuca caaacaaaua aaguucuagu ccaucgaaaa     1980

ugcaacagca caaaaacaaa ccaaacagcc uacgacuaua uugccacaaa aggagaugaa     2040

caagucagag uauacucuga acgcguggau gauugguuua aaagaggaau cauaaguuac     2100

agcaaaaugc aacgucuaaa agucucccac aaagaauacu uagaacgaaa gaaacuagga     2160

aaagaaacug aaacagacaa gaaacucugg gaaaacuuua uagauagaca acuaagggau     2220

acccaauaca uaagccgaaa aucacgagaa auuuuacaaa aagucugcaa caacguagca     2280

acaaccgaag gcggaguaac agcaaaauua agaaaucucu ggggauggga cgacguacua     2340

augaaucuac aaaugccaaa guacaaagaa cuaggacaaa caguaguuaa agaauggaca     2400

ucagaacaug gcaaacgaaa acaucaaaaa gaagaaauag aaaacuggac aaaacgcgau     2460

gaucaucgcc aucaugcgau cgacgcauua gucguugcau guacaaaaca aggauucaua     2520

caaagaauaa auacacuuaa cucaucagaa acgaaagacc ucaugcaaca agaaauuaaa     2580

gaagccaaga aacuauuagg agaaaacuac aaugaaaagg aauuugaaaa caacaacgaa     2640

ucuguuaaaa accacaaaga acgccucuca cagcucgacg acuacuuaag gcuacaaaaa     2700

ccaguaaaau ucgacacaaa auauguagaa caagaagcug auaaaguauu aguaucauuc     2760

aaaucaggua agaaaguagc uacuauaacu aaauucaaag ccacagguaa aaauaaagau     2820

acaggcguaa ucguaccacg cggcgcccua cacgaacaga acguauacgg caaaauuaaa     2880

gugauagaaa aagauaaauc acuuaaauau cucuuugaaa auucagagaa aauaguaaac     2940

ccaaaaauca aaaacauagu agaaauuaua cuaucugaaa acgaaaacaa caguaaaaug     3000

gcauuaagca cucucaagaa aaauccaauu uuucuaaacg aagaaaagac agacuuauua     3060

gaaaaagcgu ccugcuauga auacguaaca gugagaaaau auccacuaaa uacgauuaaa     3120

gcaagccaag cagacgauau agucgaugaa agaguaaaau uccuagucaa agaacgucuu     3180

acacaauuug gcaauaaaga aaaagaggcu uuuaaagaca cauuaugguu caaugaagaa     3240

aaacaaaucc ccauaauuac aguccgacuc uuugcuaggc cugacgcaaa uaaccugcag     3300

gcuauuaaga aagacgaaag cggaaaagaa auugguuucg uaguaauggg aaauaaucac     3360

cauauagcaa uuuaugaaga uaaagacggg aagcuaguac aacauucaug cacauucugg     3420

cacgccguag aaaggaagaa auacaacaua uccacgguca ucaaaaacuc aucagacgca     3480

uggaaccaau ucuuggacaa ugaacugcca gagccauuuu uaaauaaauu accagcagac     3540

aaucuaaauc uaaaauucag ucuacaacaa aaugaaaugu ucauauuagg gcuaucgcaa     3600

gaggaauuug acaaagcauu aaaaucgaau gauaaaagcc uguuauccaa acaccucuac     3660

uugguauggu cuauuucuga aaacaauuau ugguucagac accauuugga aaccaaaaac     3720

ucggaccuaa agaaaacaaa cggcguaaaa gaaaguaaaa gacuauauaa uauacgaagu     3780

cugggaaguu uauuuucucu aaacccuaua aagauaagac ucaaucacuu aggagaaauc     3840

acuaaaauag gcgaguacuc auga                                            3864


<210>  5239
<211>  3867
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5239
augcguuuau uugaagaagg acauacagca gaagacagac gacuaaaacg aacagcucga       60

agaagaauau cccgccgccg uaacagauua agauaccugc aggccuucuu ugaagaagcu      120

augacagacc uagacgaaaa uuucuucgca cgauuacaag aaucuuuccu aguacccgaa      180

gauaagaaau ggcaccguca ucccauauuu gcuaaacucg aagacgaagu ugcauaccac      240

gaaacuuauc caacaauaua ccacuuaagg aagaaauuag cggauucauc ggaacaagca      300

gacuuaagac ucauauaccu cgcacucgcc cauauaguua aauacagagg acacuucuua      360

auagaaggca aacugucuac cgaaaauacu ucaguaaaag aucaauucca acaauucaug      420

guuauuuaca accaaacguu cguaaaugga gaaaguagau uaguaucagc gcccuuaccu      480

gaaucugugu uaaucgaaga agaacuaaca gaaaaagcau ccagaacaaa gaaaagcgaa      540

aaaguacugc aacaauuucc acaagaaaaa gccaauggcu uauucggcca auuuuuaaaa      600

cuaaugguag guaacaaagc agacuucaag aaaguauucg gacuagaaga agaagcaaaa      660

auaacguaug cauccgaaag cuaugaagaa gauuuagaag ggauccuagc gaaaguaggc      720

gaugaauaca gcgacguauu ccuagcagcg aaaaacguau augaugcagu ugaauuauca      780

acaauucuag ccgacaguga caagaaauca caugccaaau uaucuagcag uaugauugua      840

agauucaccg aacaucaaga agaccucaag aaauucaaaa gauucauaag agaaaauugc      900

ccagaugaau acgauaaccu auuuaaaaac gaacagaaag augguuacgc aggauacaua      960

gcacacgcag gaaaaguuuc ccaacuaaaa uuuuaucaau acgucaagaa aauuauucag     1020

gauauagcug gagccgaaua cuuccuugag aaaauugcac aagaaaacuu ccuacggaaa     1080

caaagaacuu uugacaaugg cguaaucccu caccaaaucc acuuagcaga acuacaagcc     1140

auaauucaua ggcaagcagc auauuacccu uucuuaaaag aaaaccagga gaaaauagaa     1200

caguuaguaa cauuuagaau accauacuau guagguccac uaucuaaagg agacgcaucc     1260

acauucgcau ggcuaaaaag acaaucagag gaaccaauua ggcccuggaa cuuacaagaa     1320

acaguagauc uugaccaauc ugcgacagca uuuaucgaaa gaaugacuaa cuuugauaca     1380

uacuugccau ccgaaaaagu gcuaccgaaa cauucuuuau uauaugaaaa auucauggua     1440

uucaacgaac ucacaaaaau aucauauaca gacgauagag guaucaaagc aaauuucuca     1500

gggaaagaaa aagagaaaau uuucgauuau cucuuuaaaa cccgcaggaa aguuaaaaag     1560

aaagacauaa uacaguucua ccgaaacgaa uauaauacag aaauaguuac gcuaucagga     1620

uuagaggagg accaauucaa cgccagcuuu ucuaccuauc aagaucuuuu aaaaugcggg     1680

cugacaaggg cagaacucga ucacccagac aaugcagaaa aauuagaaga caucauaaaa     1740

auacuaacca ucuucgagga ucggcaaaga auaagaacac aacuuagcac auucaaaggc     1800

caauuuucgg cagaaguacu gaagaaacuu gagaggaaac acuauaccgg uuggggacgg     1860

uuaucuaaga aacuaauaaa cgguauuuau gauaaagaau caggcaaaac cauauuagau     1920

uacuuaguaa aagacgaugg cguaucaaaa cacuauaauc gcaauuuuau gcaauuaauu     1980

aaugauucac aacuauccuu uaaaaacgca auacaaaaag cacaaagcuc cgaacacgaa     2040

gaaacgcuau cagagacagu aaaugaacuc gcagggucac cagcaaucaa gaaagggauu     2100

uaucaaucgc uaaaaauagu agaugaauua guagcaauua ugggguacgc cccaaaaaga     2160

auaguagugg aaauggcaag ggaaaaucaa acaacaagua ccgguaaaag aagaagcauu     2220

caaagacuca aaauagugga aaaagcuaug gcagaaauug gcaguaaucu guuaaaagaa     2280

caaccaacua cuaaugaaca auuaagagau acaagauuau uccuauauua caugcaaaac     2340

ggcaaagaca uguauacagg ugacgaacuc uccuuacaua gauuaucaca uuacgacaua     2400

gaccauauaa uuccccaguc cuuuaugaaa gacgauucuc uagacaacuu aguauuaguu     2460

ggcuccacag aaaacagagg gaaaucagau gacgucccua guaaagaagu aguaaaagac     2520

augaaagcau acugggaaaa auuauaugcu gccggacuca uaucacaaag aaaauuccaa     2580

cgccuaacua aaggugaaca gggaggacua acucucgaag acaaagcgca cuucauacaa     2640

aggcaacuug ucgaaacacg acaaaucacc aaaaacguag ccgggauacu ugaccaaaga     2700

uauaaugcua aaucuaaaga gaagaaaguu caaauuauca cccuaaaagc cucuuuaacu     2760

ucacaauuca gauccauuuu cggccuauau aaaguucgag aaguaaauga uuaccaccau     2820

gggcaagacg cuuaccuaaa uugcguaguu gcuacaacau uacuaaaagu cuacccuaac     2880

cuagcccccg aguucgucua uggugaauau ccuaaauucc aaacauuuaa agaaaacaaa     2940

gcaacagcaa aagcaauaau uuauacaaac cucuuacgcu uuuucacaga agacgaaccg     3000

agauucacua aagacggaga aauccuaugg uccaauucau accucaaaac uaucaagaaa     3060

gaacuuaauu aucaccaaau gaauauaguc aagaaagucg aaguccagaa aggcgguuuc     3120

agcaaagaau caauaaaacc aaaagguccc aguaauaaac uaauaccagu caaaaacggu     3180

cuagacccac aaaaauacgg aggguucgac agcccuguag uagcauauac uguccuuuuu     3240

acacacgaaa aaggcaagaa accacuaaua aaacaagaaa uacuuggaau caccauaaug     3300

gagaaaacaa gauuugaaca aaauccgauc cuauuccuag aggaaaaagg uuuccugaga     3360

ccaagaguau uaaugaaacu cccuaaauac acauuauacg aguucccaga agguagaaga     3420

agguuacugg cauccgcaaa agaggcucaa aaaggcaauc aaauggucuu accagaacac     3480

uuacucacuc uauuguacca cgccaaacaa ugccuccuac caaaucaguc cgagucucua     3540

gcauacgucg aacaacauca acccgaguuc caagaaaucc ucgaaagagu agucgauuuc     3600

gcugaagugc acacacucgc caaaucaaaa guacaacaaa uaguuaaacu auuugaagcu     3660

aaucaaacug ccgacguuaa agaaaucgca gcaucuuuca uacagcuaau gcaauucaau     3720

gcaaugggug ccccaaguac uuuuaaauuc uuucaaaaag acaucgaaag agccagguau     3780

acgucuauua aagaaaucuu ugaugcaaca auaauauacc agagccccac aggauuauac     3840

gaaacaagaa gaaaaguagu cgacuga                                         3867


<210>  5240
<211>  3903
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5240
auguggggag uuucacuauu ugaagccggg aaaaccgccg cagaacguag ggguuaucgg       60

agcacacgaa gaagacuuaa ucacaggaaa uuccguuuac gacuuuuaga agacauguuc      120

gaaaaagaaa uauuaucaaa agauccaagu uucuucauaa gauugaaaga ggcuuuccuu      180

agcccaaaag acgaacaaaa acaauucaaa ggaaacauau uauucaacga caaagacuau      240

accgaugcag acuacuacga acaauacaaa accaucuauc acuuacguua cgauuuaaua      300

ucacaacauc gccaauucga uauacgcgaa guguaccugg cgauacacca ccucauaaaa      360

uacagaggcc acuuuauaua cgaggaccaa acauuuacua cugacgguaa ccaacuucag      420

caucauauaa aagcaauaau cacaaugauc aauuccacau uaaaccgcau caucauacca      480

gagacuauag auauaaacgu auucgagaaa auccuucuag accgaaugau gaaccgcucg      540

aguaaaguaa aauuucuaau agaacuaacu ggagaagaca aacaagauaa gcccuuacuc      600

aaagaacugu uuaaccucau cguuggacua aaagccaagc cagcaucuau cuucgaacaa      660

gaaaaccuug caacaauagu cgagaccaug aacaugucaa cagagcaagu gcaacuagau      720

cuccucacuc uagcagacgu ucuugccgac gaggaauaug acuuacuucu aacagcacag      780

aaaauauauu cagcaaucau ccuugacgaa ucaauggaug gauaugaaua uuuugcagaa      840

gcaaagaaag aaucauauag aaaacaccaa gaagaacuug uauuaguaaa gaaaaugcuc      900

aaaucaaacg cuauaacaaa cgaugagaga gcaaaauuug aauacuucua cacagacuau      960

aucggagcaa aaucaagcaa cuaugaggaa ucuaaaaaca ucaagaaagg ccuuagugcc     1020

gcauacggga aauauagcaa agaagaaagg uuauucaaac auauagaauu acuacuagcu     1080

aaagaaaacg uccuuacaac uguagaacau gcacucuuag agaaaaauau aacauucgcu     1140

uccuuacuac cucuccaacg uucgucuaga aacgccguaa uaccauacca aguccacgaa     1200

aaagaauuag uugcuaucuu agaaaaccaa gcaacauacu auccauucuu acuagaacaa     1260

aaagacaaua uacacaaacu gcuuacguuu agaaucccau auuacgucgg accacuugcg     1320

gaccaaaaag acagugaguu cgccuggaug guacgaaaac aagcugguaa aaucacacca     1380

uuuaacuucg aagaaauggu cgauauugac gcaagcucag aagcauuuau aaagcgaaug     1440

acuaauaaau guacauacuu aauucacgaa gacguaauac caaaacauuc cuucucauac     1500

gcaaaauuug aaguccuaaa ugaacuaaau aaaauacgac uugacggaaa accaauagau     1560

aucccauuaa agaaaaggau uuuugaagga cuauuucucg agaaaacaaa aguaacacaa     1620

acaucacuga agaaauggcu agcagaacac gaacacauga cagucucagu uguucaaggc     1680

acacaaaaag aaacagaauu ugcuacauca cuccaggcgu uccaccgauu ugugaaaauc     1740

uuugaucgcg aaacgguauc aaacccagca aaugaagaaa uguucgagaa aauaauauau     1800

uggucaacag uuuuugaaga caagaaaaua augagaagaa aacuaucaga auauccacaa     1860

uuaaccgaac agcaacaagu acaauuagca caaguacgau uuagaggcug gggaagacua     1920

ucgcaaagau uaauuaacag aaucaaaacg ccagucucug gagacgaaga ccauaaauua     1980

ucgauaaacg aaaucuuaug gcaaacaaac gaaaacuuca ugcaaauaau acgaaacaaa     2040

gacuaucuau ucaagaaaau aauagaagaa caauucgaaa acgaaaccgc auugcucaau     2100

aaacaaagaa uagacgaacu agcagcguca cccgccaaca agaaaggaau auggcaagca     2160

auaaaaaucg uuaaagaacu agaaaaaguc cuacaacaac ccgcggaaaa cauuuucaua     2220

gaguucgcua gaucagacga agaaaguaaa cgaaguaccc caagagacaa auucauagaa     2280

aaggcuuaug cucaacuaaa gaaagaaacg gauacauuca acuuagaaca ccuaaaagaa     2340

uuaaaacagc gaaguaaaca acuaaguagc caacgauuau uucuauacuu uauccaaaac     2400

ggaaaaugua uguauucagg cgaacaccuu gauauagaac gacucgacuc uuaugaaguc     2460

gaccauaucc ucccucaauc cuacauaaaa gacaacucca uagaaaacuu ggcacugguc     2520

aagaaaguug aaaaccaacg aaagaaagac ucuuuacuac ucaauaguuc aauaauuaac     2580

cagaauuauu ccagauggga acaacuuaaa aaugcaggac uaauaggaga gaagaaauuu     2640

agaaaccuaa caagaacaaa aaucacagac agagauaaag aaggauucau agccagacag     2700

cuaguagaaa ccagacaaau cacaaaacac gucacacaac uacuacaaca agaauauaaa     2760

gacacaacaa aaguauucgc gauuaaagca acauuagucu caggccuucg aagaaaauuc     2820

gaguucauca aaaacagaaa cguaaaugac uaucaucacg cccaagacgc cuucuuaguu     2880

gcauuccuug guacaaacau cacaucaaac uacccaaaaa ucgaaaugga auaucuuuuu     2940

aaagguuacc aacauuaccu aaacgaagua ggcaaaagug ccgcuaaacc aaaauuuaca     3000

uucauaguug aaaaccucag uaaacaacaa uacaacucaa cgacaggaga aguuaaaugg     3060

aacccugaag ucgacauagc aaaauuaaaa cgcauacuca acuucaaaca guguaacaua     3120

guacguaaag uagaagaaca aucuggagca cucuuuaaag aaacaauaua uccagucgaa     3180

gaaucuaguu cuaaaaccau accacugaag aaacaccucg acacagccau auaugguggc     3240

uacacugcag uaaauuaugc aucuuaugca cuaauacaau uuaagaaagg ucgaaaauua     3300

aaacgagaaa ucaucggcau cccacuagcu gugcaaacac gcauugauaa cagcgaaaca     3360

ucguuacaag cauauauugc agaacaaauc aaaucagaag uagaaauauu aaacggccgc     3420

auacuuaaau aucaauuaau cagcaauaau ggaaaucgac uuuauauagc aggaccauca     3480

gagcgacaca augccagaca auuaauagua ucagacgaag cagcaaaagu aauauggcua     3540

aucucaacaa aacaggccga cgaagcaaug uuccuaaaau auuauagauu agagcaccuc     3600

gaagccguuu ucgaagaacu cauacgaaaa caagccgcgg auuaucaaau cuuugaaaaa     3660

cuaauaaaga aaaucgaagu aaacaaaguu uacuuuuaua gcugcacaua caacgaaaaa     3720

guuaaaguaa uagaagaacu acuaaaaaua acacaagcca acgcaacaaa uggagaccuc     3780

aaacugcuca aaaugucaaa ucgcgaagga cgacucgggu caguuucagu agcccuggac     3840

uuuaaaauaa ucaaccaauc aguaaccgga uuauaucagu caaucgaaga cuauaacaac     3900

uga                                                                   3903


<210>  5241
<211>  3930
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5241
augggaucug ugggguucgc uggaauugau aaccaauaua acuuaguuag aaccaaagga       60

aagaauguaa uaggagucag guuguuugac gaagccgauu ccgcagcaga acgccgagga      120

cauagaacaa auaggagaag gcuacaaaga agaagauggc gcuuacgcuu acucgaugac      180

auauucgcaa aaccacuaca agcaguagac ccaaauuucu uagcacgccu uaaauauuca      240

uauguaaaua aaaaagacca gggacaacag gaccauuacu auggcggaua uguuuuugga      300

aguaccgccg cagaucaagc auaccaccaa gccuacccga ccauauacca ccuaaggaaa      360

agacucaugg aagaugauca aaaacaugau uugagagagg uauacuuagc aauacaucac      420

auaguaaaau acagagguaa cuuuuugaau ccacaaagua gccucgacau agaucaacaa      480

uuugacguaa cagauuucgc acaagcacuu gcgagauuug cagaccauca agcacucagc      540

ugggcauuag aagcuccaau uagauuuuua gaagcugaac uagcaacugg acuaucgaac      600

ucugcccgug uagaugccgc uaucgaggca uucagcuuug auacaaaagu ugacagagcc      660

gccauaaaag aaauguuaaa aggcuuaucc ggaaaucaaa uagacuucac caaacuauuc      720

guaaauguug auucagccga cugggaucaa gaagaaagaa aacaguggaa aaugaaacuc      780

ucagaagaag acuucgacga acaagcgcua ccaauauuag aaagauuauc ucaagaugag      840

acugaauuuu uucuugcaau uaaacgagca uaugacggaa uugcccugau gcgauuucua      900

ggugaugaac aaucucuuuc uuccgcuaug auaaaagcuu acgaagacca uagacgagac      960

cuaacuuucu uaaaaacaca agugcguaca ccacaaaaua gacaagcacu cagugaagga     1020

uauacgaacu aucugucugu agaugacaaa aagcauaaaa ggggagccaa agaacuugca     1080

caauuaaucg aagcaagcga ugcaucugaa caagacaaag caacaaugcu agaccggauc     1140

gcuaacgauc aauuugcccc aaaacagaga acaaaagcua acggcuuaau uccuuaccaa     1200

cugcacuuag cagaacuaaa aaaaauauua gcgaaacaag gucaauauua uccauucuua     1260

uuagauaccu ucgcaaaaca aggccagagc guuaacaaaa uagaagaacu cguacaauuc     1320

agaguaccau auuacguagg uccaauggua ccuaaaucug aaaccgcagg aaaugcagaa     1380

aaucacuggg uugaaaaaaa ugauggacaa acaaaaguaa gcguuacccc auggaauuuu     1440

gaccaaguuu uuaaccgcga uagggcagca aaaucauuca uagauagauu aacaggaacc     1500

gauacauauu uaauagguga accuacuuua ccgagacauu cacuaacgua ugaaaccuuu     1560

acaguauuaa augagcuaaa uaacauaaga aucgauggga aaagauuacc agucgaaacg     1620

aaacaagcaa uaguagaaga ucuauuuaaa aaauaucggc uaguaacaaa aaaaagacuu     1680

caagacuacu ucgcaucauu uggcaaaaga gaaguagaac ucaccggacu ugcagacgaa     1740

ucacgauuca ccucuaguuu aaccucuuau cacgauuugc aaggcuuauu aggaacagac     1800

uucauaacaa acccacaaaa ccauucacua cuggaaaaaa uuguagaaau ucagacuguc     1860

uuugaagauu ccgauauugc ugaacgcgag cuuggcaaau uaggcuuaga acaaaaauua     1920

auaccacguc uagcaaaaaa acacuacacu gguuggggaa aucuuucaag aaaauuguua     1980

gacaccaguu ucauacauga ucccgaaaga ccagaagaac cgguaucaau aauggaucug     2040

cuauacacaa caaacaaaaa cuucauggag auacuacaug auucagaaua cggaguagaa     2100

gaaugguuga aaucccaaaa uaugaucgac gaucaaaaag auauacaaau gcgaauagau     2160

gaacuuacua caucacccgc caauaagaga ggaauaaaac aagcuuucaa uguauuggau     2220

gauauaacuc aagcaauggg ggaagaacca gcuuacguau accuagaauu ugcaagagaa     2280

aaacaagcau cacgcagaac ggugucacga aagaagcgau uagaaacucu auauaagaau     2340

gccgcucuaa aaacagaguu uaaagcaaua aaagaagcgu uagcugaaga aagcgaugac     2400

cgcaugcaag acgaucgauu auaucuuuac uaugcacaau uaggaagaga uauguacacc     2460

ggacaaucaa uauccauuga ucaacuuagc ucucacuaug acauagauca caucguccca     2520

cgugcguuca uuaaagacga uaguuuagaa aauaaagucc uaguaaauag aaccgauaac     2580

gcaagaaaaa ccgacagcgc aacauucaca gcagacguua aagccaaagc auucccuuua     2640

uggcaacaac uaaaaaaacu aggacucaua agugccaaaa aauuucgucu acuaacaaga     2700

acaggugauu ucacggaaau ggaaagggaa agguucauag ccagacagcu uguugaaaca     2760

agacaaauaa uaaaaaaugu agcggcccua auagaaggac auuucaguca gacccaagcu     2820

guagcuauaa gagcggaagu uacuggugaa cuacgacagc ucacacagau aaaaaaagau     2880

cgugacauua acgauuauca ccaugcucaa gaugcacucc uaguugcaac agcuggcacu     2940

uauuuacaua gacacuuucc uaagcgugac gcucgcuuua uauauaacga auucgacuau     3000

uauacacaac acuggcuaaa aaaccaagga gagaaucgaa gacgucaccc auacucauuc     3060

gugguaggaa caaugucuaa agggaacgaa gacuggacgc cagauaacuu aaacuauuua     3120

cgaaaaguaa ugcaauacaa gacaauguug augaccagaa agccgguagg gccagaaggu     3180

gcauuauaua aagaaacuuu aauugcugcu gauccuaaga aaagacuugu aggagcuucc     3240

aaagaaagac aagauccaac caucuacggc gguuacacaa aagaaucauc agcuuacaug     3300

ucauuaguac gagcuggagg uaaaaaucaa uuggucaaaa uaccuguuag aaucgcaaac     3360

gagauacaca guggacaacg aaaguuagau gauuacgucc aagcaaaagu aaaaaaguuu     3420

gagaggaucu uacuuccaaa aaucucgcuu gggcaauugg ucgaagauga agggcagcga     3480

uuuuaucuag cuaccaacga aaugaaacac aacgccaaac agcuaugguu agaccaaaaa     3540

gucgucacaa cauacaaacg acucacagca gaaucaccag uagaagauuu ccucacggua     3600

uuugaugcac uaacaucaag ugcuacuaua caucacuuua aauucuauca acgagauuug     3660

gaacuauuac gagacaauag agcaggauuc caagacuuag caaaagcuac acagcuuaaa     3720

guguuaaagg acguccuaua ugaauuacac gauaaugcug gauggcgaga uccaauaaaa     3780

caauacuuua aagaaauagg auuaaaaguu cgaaugugga caaaacuaca aaaagaaggg     3840

ggaaucaaac uuacugauca agcagaacuu auauaccaau cacccucagg acucuucgaa     3900

aaacgaagaa gaguacaaga uuuauuauga                                      3930


<210>  5242
<211>  3939
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5242
augcaaaaug gauuucuugg aauuacagua ucaucagaac aaguaggcug ggcugucaca       60

aauccaaaau augagcuaga acgagccagu agaaaagauc ucuggggagu aagacuauuu      120

gauaaagcug aaaccgcaga agacagacgg auguucagga cuaacagaag acuaaaucaa      180

cgaaaaaaaa auaggauaca uuacuuacga gacauuuucc augaagaagu uaaccagaaa      240

gacccaaauu ucuuucaaca auuagacgaa ucuaauuucu gcgaagauga ucgaacggua      300

gaauuuaauu ucgacacaaa ccucuacaaa aaccaguuuc caacuguaua ccaucuacgu      360

aaauaucuaa uggaaaccaa agacaaaccg gauauacgcc uaguuuaucu cgccuucagu      420

aaauucauga agaauagagg acauuuccua uauaagggaa acuuaggaga agucauggac      480

uucgaaaacu cuaugaaagg auucugcgaa agucuagaaa aauucaacau cgauuuuccu      540

acacugucag acgaacaagu gaaagaggua cgagacauuu uaugcgauca caaaaucgcc      600

aaaacaguua agaaaaaaaa uaucaucaca auaacuaaag ugaagaguaa aacagcgaaa      660

gcauggaucg gccuauucug uggcuguuca guaccuguaa aagugcuauu ucaagauaua      720

gaugaagaaa uaguaaccga cccggaaaaa aucagcuucg aagacgcauc auaugaugau      780

uacauagcga auauugaaaa aggaguaggu auauauuacg aagcaaucgu aagugcaaag      840

auguuauucg auugguccau aaugaacgaa auacuaggag aucaucaauu acuuucagau      900

gcaaugauag cagaauacaa caaacaccac gacgaucuga aacgacuuca gaaaauaaua      960

aagggaacug gcagucgaga acuauaccaa gacauauuua uuaacgaugu aaguggaaac     1020

uacgucugcu acguagggca ugcaaaaacu augaguucag cagaucaaaa acaauuuuac     1080

acauuucuaa agaaccgccu aaaaaacguu aacgggauau caagcgaaga ugcagaaugg     1140

auagauacag aaauuaaaaa cggcacauua cuaccaaaac agacaaaacg agauaacuca     1200

guaauaccac accaauuaca acuucgugaa uuugaacuaa uacuagacaa uaugcaggaa     1260

auguacccau uucuaaaaga aaauagagaa aaacucuuaa aaauuuuuaa cuucguaaua     1320

ccauauuacg uaggaccgcu aaagggcgua guacgaaaag gagaaucuac uaauuggaug     1380

gucccaaaaa aagacggagu aauccauccg uggaauuuug augaaauggu agacaaagaa     1440

gccagugcag aaugcuucau aucgagaaug acagguaauu guaguuaucu cuucaacgaa     1500

aagguacugc ccaaaaacuc auuacuauac gagacauuug aaguacuaaa ugaacucaac     1560

ccacugaaga ucaauggaga accaauauca guagaacuaa aacagaggau auaugaacaa     1620

uuauuucuaa cuggaaaaaa agucaccaaa aaaucccuca caaaauauuu gauuaaaaac     1680

gguuaugacg aagacaucga auuaucaggu auagauaaug aauuucacuc aaauuuaaaa     1740

ucucauauag auuucgaaga cuacgacaac cuaucagacg aagagguaga acgaauaauu     1800

cuuagaauca caguauuuga ggacaagcaa uuauuaaaag acuaucucaa cagagaauuc     1860

guaaaacuga gugaagacga acgaaaacaa aucugcucgc uuucauauaa aggaugggga     1920

aacuuaucag aaaugcucuu aaauggaaua accguuacug acaguaacgg cguagaagua     1980

ucagucaugg acauguuaug gaauaccaau cuuaacuuaa ugcaaaucuu gucaaaaaaa     2040

uauggauaca aagcggaaau agaacgauau aacaaggagc augagaaaaa cauauacaau     2100

agagaagacu uaauggauua ccuuaauaua ccaccugcuc aaagaagaaa aguuaaucaa     2160

uuaauuacaa uagucaaauc ccuuaaaaaa agaugcggug uaccaaacaa aauauuuuuc     2220

aaaguaucaa gagaacauca agacgauccc aaacggacua gcucacguaa agaacagcuc     2280

aaguaucuau auaaaucacu uaaaucagaa gacgaaaaac acuugaugaa agaacuagau     2340

gaacuuaaug gucaugaacu uucaaaugau aaaguauauc uuuauuuucu ccagaaaggc     2400

agguguaucu acagcggaaa aaaauuaaac cuaagccguu uacgaaaauc aaauuaccag     2460

aacgacauug auuacaucua uccauuaagu gcaguaaaug accgcagcau gaauaacaaa     2520

gucuuaaccg guauccaaga aaaccgagcc gacaaauaca cauacuuccc uguagaccca     2580

gaaauacaaa aaaaaaugaa aggauucugg auggaauuag uccuucaagg cuuuaugagu     2640

aaagaaaaau acuuccgacu auccagagaa aacgauuuca gugaaaauga auuaguaagu     2700

uuuauugaaa gagaaauaag ugacaaucaa caaucuggac gaaugaucgc uagcgucuua     2760

caauauuacu uuccggaauc uaaaauagug uucguuaagg agaaacuaau aucaucauuu     2820

aaaagagacu uucauuuaau aucaaguuau ggacauaacc accuacaagc cgccaaggac     2880

gcauauauua caauaguagu agggaaugua uaccacacca aguucacaau ggaucccgcc     2940

auauauuuca agaaucauaa gcgaaaagau uacgaccuaa aucgauuauu uuuggaaaac     3000

aucucgagag acggacagau agccugggaa ucuggaccau acgguucuau acaaacaguc     3060

agaaaagaau acgcacaaaa ccacauagcc guuacaaaaa ggguaguaga aguaaaaggc     3120

ggccuguuua aacagaugcc cuuaaaaaaa gggcacggag aauauccauu aaaaacuaac     3180

gacccaagau ucggaaacau ugcacaauac ggcgguuaua caaacguaac gggaagcuac     3240

uucguguuag ucgaaagcau ggaaaaagga aaaaaaagaa ucagucuuga auauguacca     3300

guuuaccuac acgaacgacu cgaggaugac ccgggacaua agcucuuaaa agaauaccuu     3360

guugaucaua gaaaacuaaa ucauccgaaa auauuacugg cuaaaguaag aaaaaacagc     3420

uuauuaaaaa ucgacggcuu cuauuaucga uuaaauggaa gauccggaaa ugcguuaauu     3480

cucaccaacg ccguagaauu aaucauggac gauuggcaga cuaaaacugc aaacaaaaua     3540

aguggcuaca ugaaaagacg ugcgauagau aaaaaagcga gaguauacca aaaugaauuc     3600

cacauucaag aacuagaaca acuauaugau uucuacuuag acaaacuaga aaauggagua     3660

uauaaaaaca gaaaaaauaa ccaagcagaa cucauacaca acgagaaaga acaguucaug     3720

gaauuaaaaa cagaagauca augcguuuug cuaacagaaa uuaaaaaauu auuuguuugc     3780

ucucccaugc aagcagaucu gacacuaaua ggaggaucaa aacacacagg aaugauagcc     3840

augagcucaa auguaacaaa agcagauuuc ucaauuauag cugaagaccc uuuaggcuua     3900

agaaauaaag ugauauacuc acacaugggu gagaaauga                            3939


<210>  5243
<211>  3939
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5243
augcagaacg gcuuuuuggg aauaacagua uccuccgaac aagugggaug ggcaguaacg       60

aacccuaaau augaacucga acgagcguca cguaaagacc uauggggagu aagacucuuu      120

gacaaagcag aaacagcaga agaccgacga auguuuagga caaauagacg auuaaaucag      180

agaaaaaaaa acagaauaca uuaccucaga gauauauuuc augaagaagu caaucaaaaa      240

gacccaaauu ucuuccaaca acuggaugaa ucaaacuuuu gcgaagacga ucgcacggua      300

gaauucaacu ucgacaccaa uuuauauaaa aaucaguucc caacagucua ccaucuacga      360

aaauaucuaa uggaaaccaa agauaaacca gacauaagac uaguauaucu ggccuuuagu      420

aaauuuauga aaaaucgagg ccacuuccua uacaaaggua aucuaggcga aguaauggac      480

uucgaaaaua gcaugaaagg uuuuugcgaa aguuuagaaa aauucaauau cgauuuucca      540

acacucucag acgagcaagu aaaagaggua agagauauac ucugugacca caaaauagca      600

aaaaccguaa aaaaaaaaaa cauaauuacg auuaccaagg uaaaaagcaa aacggcaaaa      660

gcuuggauag gacuauucug uggauguuca guacccguua aaguacuauu ccaggacaua      720

gaugaagaaa ucguaaccga uccagagaaa aucagcuucg aagacgcauc auaugacgac      780

uacauagcua acauagaaaa gggcguagga auuuauuacg aagcaauugu cagcgcaaag      840

auguuauuug auuggaguau auuaaacgaa aucuuaggug accaccaauu auuaucagau      900

gcaaugauag ccgaauauaa uaaacaucau gacgacuuaa aacgacuaca gaaaauaauu      960

aaaggaacgg gaucacgaga acuauaccaa gacaucuuca uaaacgacgu guccgggaac     1020

uacgucuguu auguaggaca ugcaaaaacc augaguuccg cagaucaaaa acaauuuuau     1080

accuuucuua aaaacagacu aaaaaaugua aauggaauau caagcgaaga cgcagagugg     1140

auagacacag aaauuaagaa cggcacuuua cuucccaaac aaacaaaaag agauaauagc     1200

guaauaccac accagcuuca acuucgagag uuugagcuaa uucuugauaa uaugcaagag     1260

auguauccau uuuuaaaaga aaacagagaa aaauugcuaa agauauucaa uuucguaaua     1320

ccauauuaug uaggaccacu uaaaggagua gugagaaagg gcgaaucaac uaacuggaug     1380

guaccuaaaa aagacggagu aauccacccu uggaauuucg acgagauggu agacaaggag     1440

gcaagugcag aaugcuuuau aucuagaaug acaggaaacu guaguuacuu auucaacgaa     1500

aagguauuac caaaaaauuc acuauuauau gaaacauucg aaguucuuaa cgaauuaaau     1560

ccauuaaaaa uaaacggaga aagcauaagu guugaguuaa aacaaagaau auacgaacaa     1620

uuauuccuaa cgggcaaaaa agugacgaag aaaucccuga caaaauauuu aauaaagaac     1680

ggauacgaua aagacauaga acuaucuggc aucgacaaug aauuccauag caaccuaaaa     1740

ucgcacauag acuuugaaga uuaugacaau cuaucagacg aagagguaga acaaauuauu     1800

uuaagaauaa cagucuucga ggacaaacaa uuacuaaaag auuacuuaaa uagagaauuu     1860

guaaaacuuu cagaagauga aagaaaacaa aucugcucau uaaguuauaa aggauggggu     1920

aacuuaagcg aaaugcuauu aaauggaauc accgucacug auucaaacgg gguagaaguu     1980

ucaguuaugg auaugcuuug gaacacgaau uuaaaccuca ugcaaauauu aaguaaaaaa     2040

uacggauaua aggcggaaau agaacauuau aauaaggagc augagaaaac aauauacaau     2100

agggaggacc uaauggacua ucucaauauc ccaccagcac aaaggagaaa gguuaaccaa     2160

cuaauaacga uaguuaaauc ccuaaaaaaa acauacggag uaccaaauaa aaucuuuuuc     2220

aaaguguccc gagaacacca agacgauccu aaacguacga guaguagaaa agaacaacua     2280

aaauaccuau auaaaucacu uaaaagcgaa gaugaaaaac acuuaaugaa agaacucgac     2340

gagcuaaaug gucacgaauu aaguaacgau aaagucuauc uauacuuucu ccaaaaaggg     2400

cgauguauau acucgggcaa aaagcuaaac cuaagcagau uacgaaaauc aaacuaucaa     2460

aacgacauag acuacauaua uccccuaagu gcagucaacg aucguucaau gaauaacaaa     2520

guauuaacug gcauacaaga aaauagagca gauaaauaca cauauuuucc aguugaucca     2580

gaaauccaga aaaaaaugaa agguuucugg auggaacuag uguuacaggg auuuauguca     2640

aaagagaaau acuuuagacu aagucgagaa aaugauuuuu cagaaaauga auuagucuca     2700

uucauagaac gcgagauuuc cgacaaucaa caauccggac gcaugauagc aucuguacuc     2760

caauacuauu ucccagaaag caaaauagua uucguaaaag aaaaacucau cuccuccuuc     2820

aaacgugauu uucaucuaau aagcucguau ggacauaacc acuuacaagc cgcaaaagau     2880

gcauacauaa cuauaguggu uggaaauguc uaucacgcua aauucacaau ggacccagcu     2940

auauauuuua aaaaccacaa aaggaaggau uacgaccuaa acagacuauu cuuagagaac     3000

aucagcagag acggccaaau agccugggaa aguggaccgu augguucaau ccagacaguu     3060

aggaaagagu augcacaaaa ccacauagca guuacuaaac gaguaguuga aguaaaaggg     3120

gguuuguuca aacaaaugcc auugaaaaaa ggccacggag aauacccacu aaaaacuaac     3180

gauccuagau uuggaaauau ugcccaguac ggcgguuaca cuaauguaac agggucguau     3240

uucguauugg uagagucaau ggaaaaagga aaaaaaagaa uaucauuaga auacguacca     3300

gucuaccucc augaaagauu agaagaugac ccagggcaua aacucuuaaa agaguauuua     3360

guagaccaca ggaaauuaaa ucauccaaag auauuauuag cuaaaguacg aaaaaauuca     3420

cuacuuaaga uagacggguu uuacuaccga cuaaacggua gaagcggcaa ugcuuuaaua     3480

cuaacgaacg caguggaauu aauaauggac gacuggcaga caaaaacagc gaacaaaaua     3540

ucugguuaca ugaaacgaag agcaauagac aaaaaggcac gaguuuauca aaaugaauuu     3600

cacauacaag aacuagaaca auuguaugac uucuaccuug acaaacucga aaacggcguc     3660

uauaagaaca gaaagaacaa ucaagccgaa cuaauucaca acgaaaagga acaauucaug     3720

gaguuaaaga cagaagacca auguguccua uuaacagaaa uaaaaaaauu auucguaugc     3780

ucaccaaugc aagcagacuu aacccuuaua ggggguucaa aacauacagg aaugaucgca     3840

augagcucaa acgugacaaa agcagauuuu gcaguaauag cagaagaucc uuuaggcuua     3900

aggaacaaag uaauauacuc acauauggga gaaaaauga                            3939


<210>  5244
<211>  3942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5244
augcauuacu uggaugagau cuucgcgccc caucuacaag aaguagauga gaauuuccuc       60

aggcgacuaa aacaaagcaa cauucaucca gaagauccag ccaaaaauca agcauucaua      120

ggaaaacuau uauuucccga uuuacuaaag aaaaaugaac gaggcuaucc aacacuaaua      180

aaaaugagag augaacugcc ugucgagcaa agggcucacu auccagucac caacaucuac      240

aaacuaagag aagcaaugau caacgaagac cgacaauuug acuugcggga agucuauuua      300

gcaguacauc auauuaugaa auacagagga cauuuccuua acaaugccuc aguggauaaa      360

uucaaagugg gaagaauaga uuuuuauaaa ucuuucaacg uacuaaauga agccuaugaa      420

gaacuucaaa auggcgaagg aucauuuacg aucgaaccau caaaaguuga gaaaauaggu      480

caacuguuac uugauacaaa aaugagaaaa uuaaaccgac aaaaagcugu agcaaaauug      540

cuagaaguca aaguagcaga caaagaagaa acaaaaagaa acaaacaaau agcaacagcu      600

augucgaaac uuguauuagg auacaaagcc gacuuugcaa cuguggcaau ggccaauggu      660

aaugaaugga aaauagauuu gucuucagaa acaucugaag acgaaauaga aaaauuuagg      720

gaagaauugu cagacgcaca aaaugacauu cuaacagaaa uaacaucuuu auucucacaa      780

auaaugcuaa augaaauagu acccaacggc augucuauau cugaaagcau gauggacaga      840

uacuggacuc acgagcguca auuggcagaa gucaaggaau accuagcaac ccaacccgca      900

ucugcgagaa aagaguuuga ccagguguac aauaaauaua ucggucaagc acccaaagag      960

cguggcuucg accucgaaaa aggacugaag aaaauacuuu ccaagaaaga aaacuggaaa     1020

gaaauagaug agcuucuaaa agcaggagau uuucuaccca aacaaagaac aucagcaaau     1080

ggagucauuc cgcaccaaau gcaccagcaa gaauuagaca ggauaauaga gaaacaagca     1140

aaauacuauc cauggcuugc cacugaaaac cccgcaacug gugaaagaga cagacaccaa     1200

gcaaaauaug aacucgacca auuagucagu uuuagaaucc cuuacuacgu aggacccuua     1260

guaacuccag aaguucaaaa agcuacaucg ggagcaaaau ucgcaugggc caaaagaaaa     1320

gaagauggag aaauuacacc auggaauuua ugggauaaaa ucgaucgagc ugaaagcgca     1380

gaagccuuca uuaaacgcau gacagucaaa gacacuuacu uauuaaauga agacguauua     1440

ccagcaaaca gccugcuaua ccaaaaauau aacguauuaa acgaacuaaa caacguuaga     1500

guaaacggaa gaagacucuc uguaggaaua aaacaagaca uuuacaccga acuauucaag     1560

aaaaagaaaa cugucaaagu cucggaugug gcaucacugg ugauggccaa aacacgagga     1620

guaaacaaac caucagucga aggauuaagu gacccuaaaa aguucaacuc caacuuagca     1680

acauacuuag aucucaaauc uauuguuggg gacaaagucg augacaauag auaucaaaca     1740

gaccuagaaa acaucauaga auggagaagu guauuugaag acggagaaau auuugcggac     1800

aaacuuacag aaguagaaug gcuaacggau caacaacgau cagcauuagu aaagaaaaga     1860

uacaaaggau ggggaaggcu aucaaagaaa cuacuaacag gaauagucga ugaaaaugga     1920

cagagaauua uagaucucau guggaacacc gaccaaaauu uuaugcaaau agucaaccag     1980

ccaguauuca aagagcaaau agaccaauua aaccaaaaag caauaacaaa ugacggaaug     2040

acucuucgag aauggguaga auccguacua gauggagccu acacaucacc acaaaauaag     2100

aaagccauuu ggcaagucgu acgaguagua gaggauauag uaaaagcagu agguaacgcc     2160

ccuaagagca uaucuauuga auuugcaaga aacgagggca acaaagguga aauaacaaga     2220

agccgcagaa cacaguuaca aaaguuguuu gaagaccaag cucaugaacu cgucaaagac     2280

acgagcuuga ccgaagaacu ugaaaaggca ccagaucuuu cagauaggua uuaccuauau     2340

uucacucaag gcggaaaaga cauguacaca ggagauccaa uuaauuucga ugaaaucuca     2400

acgaaauaug auauagauca cauacuaccu caaaguuuug uaaaagacga cucauuggau     2460

aaccgagugu uaguaaguag agccgaaaau aauaagaaaa gcgauagggu cccagcgaaa     2520

cuuuaugcag caaaaaugaa acccuacugg aaucaguugc uuaaacaggg acuaauaaca     2580

caacguaaau uugaaaaucu uacaauggac guugaccaaa acauuaaaua cagauccuua     2640

ggguuuguca agcgacagcu aguagagacc agacaaguaa ucaaauuaac agcuaauaua     2700

uuaggaagca uguaucaaga agcaggaaca gauauaauag aaacgagagc gggacucacc     2760

aaacaacuac gugaagaguu cgaccuacca aaaguaaggg aagucaacga uuaccaucac     2820

gccguugaug cauaucucac aacauuugcu ggacaauauu uaaaccgaag auacccuaag     2880

cuaaggucau ucuuuguaua cggagaauau augaaguuca aacauggauc agaccuuaaa     2940

uuaagaaauu uuaacuuuuu ccacgaacuc auggaaggug auaagucuca aggaaaaguu     3000

guagaccaac aaacuggaga acuuauaaca acgcgagaug aaguagcuaa aucauucgac     3060

cgauuacuaa auaugaaaua uauguuagua ucaaaagaag uacacgauag gucagaucaa     3120

cuuuacgggg caacaauugu uaccgccaaa gaaaguggca aauuaacgag uccgauagaa     3180

aucaaaaaga aacguccagu ggauuuauau ggagcauaua cuaaugggac uucugcuuuu     3240

augacuauua ucaaauucac aggcaacaaa ccaaaauaua aaguuauagg aguaccaaca     3300

accucugccg uaggacuaaa aaggguaggc aaaccagggu cagaaucuua caaccaagaa     3360

cuacaccgaa uaaucaaauc uaauccuaaa guaaagaaag auuuugaaau aguaguacca     3420

cacguaucau auucacaacu aauaguugau gaugauugua aauucacauu agcgagcgac     3480

acauaccaac acccagccac ucaauuaguu uugagcaaag aaagcaugga gauaauagcc     3540

gacgauuuca aauuuguaaa agaaaauaca gcaacagcag acgaacaguu aguacgaguc     3600

uucgacgaaa uaguaaacca gaugaaucac uauuuuacua uauucgauca aagaucaaau     3660

agacaaaaag ucacaaaagc acgagauaaa uucguuucuc uaccaacaga gucggaauac     3720

gaaggcgcca agaaaacaca aguuggcaaa acugaaguaa uuacaaauuu acucauggga     3780

cuacaugcaa acucagcaca aggagauuua aaaguuuuag gacuaucuac cuucggauuc     3840

uuucaaucuu caggaggucu aaaccuauca gaagaugcaa ugauaguaua ucaaagucca     3900

acaggacucu uugagagacg caucuguuua aaagacauau ga                        3942


<210>  5245
<211>  3942
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5245
auguccuuca aagaaaacuc gaaaauauau uauggauuag acauagggac agauucugua       60

ggcuggagca caaccaacga aaaauacgaa uuacuaaggu acaaaaauaa ccucaugugg      120

gguguaucac ucuucgaagc agcaagucua gcagaagaac gaagaggaca uaggacacaa      180

cggagaagau acgauagacg ccaacaaaga guagcccucu uaggagaacu uuucgcgaaa      240

gaaauccuaa aaacagaccc agauuucuuu cuccgacuaa aagaaucaag ucuauaccca      300

gaagaucgaa cuagcaaaaa cguuaacacu uauuuugaug augcugauuu uaaagauuca      360

gauuauuuua agauguaccc cacaguacac caccucauua aagagcuaag ugaaaguaac      420

aagccucacg acguaagauu aguauaccua gcuugcgccu ucauaguagc ucaccgcgga      480

cacuuccuua acggagccga cgaaaauaau guacaagcag ucuuagacuu ugauagcagu      540

uauugugaau uuacagacug guuuaaaagc aaugauauag aagauaaucc cuucucugaa      600

ucagucaaaa augaauuuuc aguaauacuc agaaagaaaa uaggaaucac ggcuaaggaa      660

agagaaauua aaaacuuacu uuucggaaca acaaaaaccc cagacuguua uaaagaugaa      720

gaauauccua uagacauuga cguauuaaua aaauucauau ccggaggaaa aacaaacuua      780

gcaaaauuau uuagaaaucc aagcuacgau gaacuagaua uccaaacagu cgaaguaggc      840

aaagcagacu uugcagauac aaucgaccua uuagcaagcu caauggaaga uacugacgua      900

ccacuacuaa gugcaguuaa agcaauguac gauuggucau uauuaauaga uguacuaaaa      960

ggacagaaaa cuauauccga cgcaaaagua ugugaauacg aacaacauaa aucggaucuu     1020

aaagcucuua aauacauagu cagaaaauac uuagaucgag cacaauauga ugaaaucuuu     1080

agaacugcgg gugaaaaacc caacuaugua uccuauucau auaaugugac agacguaaaa     1140

cuaaaacaac ucccaucaaa uuuuaaaaag aaaaacucag aagaauuuug caaauauauu     1200

aaaucaaaau uagagaaaau uaaaccggaa ucagaugaug aagccguuua caacgaacua     1260

aucgaaaaau guaacucaaa aacacuaugu ccuaaacaag ucacagacga aaauagaguu     1320

auucccuauc aauuauacua ccaugaauug uccaugauac uagacaaagc cucagccuau     1380

cuagauuuuc uaaauaaaac cgaagacggc auaucaguua aacagaaaau acugacauua     1440

augaaauuuc guauuccuua cuucguuggu ccauuaguaa aaagaaacga gacggacaac     1500

guauggauug uaagaaaggc cgaagguaga auuuauccau ggaacuuuga aaacauggua     1560

gacuuugaua aaucugaaga cggcuucauc agaagaauga ccuguaagug caccuaucuc     1620

gccggagaag acguacuacc caaauauuca cuacucuauu ccaaauauac cgucuuaaau     1680

gaaaucaaua auauuaaagu caacggcgag aaaaucuccc cagaacuaaa acaagauaua     1740

uuuaacgaac uuuuuaugaa aacgucaaga guaacaguua agaaaauaac ugaacuccuc     1800

aaaagaaaag gagccuuuuc agaagaaaaa ggagauucau uaaguggagu ggauaucaau     1860

auaaaaucau cacucaaauc auaccuugac uuucgacgcu uacuggaaaa uggaucucuc     1920

ucagaaaaug acguggaacg aaucauagaa aggauuacag uaaccaccga uaaaccacgu     1980

cuaguuaguu gguuaaaagc agaauauagc gacuuaccag cugaagacau aagauauaua     2040

agcagacuau ccuacaaaga cuacggaaga cuaucagcga aacuuuuaac aggauguuau     2100

gaguuagaua caaacacagg ggaaauaggu ggcagaagca uaaucgauuu uaugugggca     2160

gaaaacauaa acuuaaugca aauucuaagc gauucauaug guuacaaauc uuucauagaa     2220

gaggaaaaua aaaaguauua caugauaaau cccacaggau caauagcaca aacauuacga     2280

gaaauguaug uaucaccaag cguauccaga gcaauaauac gaacaaugga aaucguaaaa     2340

gaacugagaa aaauaacaaa gaaagaucca gacaaaauau uuguugaaau ggccagaggg     2400

ggaaaacccg aagagaaagg gaaacgaaca ucuucuagac gugagcaaau agaaaaacuc     2460

uaugauucag caaaagcguu uguaagcgac gaagauauau cucauaugag aucacaauua     2520

ggaucacuca gcgaugaaca acuaagacga gagaaauauu accuauacuu uauccaguuc     2580

ggaaaaugca uguauucggg agaagcaaua gauuucaguc gauuaggcga caaccacugc     2640

uacgacauag accauauauu uccccaauca aaaauaaacg acgacucgcu ucauaauaaa     2700

guauugguua aaucucaauu aaacggagag aaaagugaug auuacccaau uaaagcugaa     2760

aucagaaaua agaugcaccc cuuauggaag aaucuauucu acagagaccc aaagaauccc     2820

gcagacaaag uaaaguacga aagacuaacg agaucaacac cauuuacuga ggacgaacua     2880

gcuggcuuua uagaaaggca auuaguggag acccggcaau caaccaaggc uguugcuacu     2940

cuccuaaaag agauguuccc ugacagcaag aucguuuacg uaaaggcagg ucaaguuucc     3000

gaauuuaggc augauuucga uaugcuaaaa ugcagagaaa ucaacgacuu acaucacgca     3060

aaagacgcuu accuaaacau uguaauagga aauguacaug acgugaaauu cacaagcaac     3120

ccuaugaauu ucguuaaaaa cgcagauaaa cacuacacaa uuaagguaaa agaaacuuug     3180

aaacauaagg ucucaagaaa cggcgaaaca gcauggaacc cagaaaccga cuucgauaca     3240

gugaaaagga ugaugaguaa aaauucaguc agauacguac gcuauuguua caaaagaaaa     3300

ggaggauuuu ucaaucaaaa cccagaucga gcuggaaauc ccgaccucgc aagacugaag     3360

aaagaucuag auccaguuaa auauggagga uacaauucaa aaucaaucuc auguuuuucg     3420

cuaauaaaau guacaggggu aggagcagua aucauccccg uagaauuacu augcgagaaa     3480

agauauuuca gcgaugauuc uuucgccagu gaauacgccu auucaguacu uaaaaacgcg     3540

cucccggcaa agaacauagc aaaaauuagu auugaugaua uuaguuuccc guuaaagaga     3600

aggccaauaa agaucaacac gcuauucgaa uuugacggau acagaguuaa cauucguagc     3660

aaagauucau aucacuacuu uacgauaucu agcgcuaugg cugcuauaua caguaaagau     3720

acaucugacu auauaaaagc ugugagcagc uacauugaua aaucugacaa gggaucaaag     3780

uucaaaccug gagaagcguu cgacguacuc ucuaaccuaa aggucuacga ugaaaucgcu     3840

aagaaaugua uaucggaacc auuuagcaaa auaagcaaac uagcagaagc aggaaaaaga     3900

uggaagaaag aagaaauauc acuacaaaac ugcuuacucu ga                        3942


<210>  5246
<211>  3945
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5246
augcucaaaa aggacuacag cauaggucua gauauuggaa caaacagugu aggacaugca       60

guaguuaccg augauuauaa aguaccuacu aagaaaauga aaguauucgg cgacacauca      120

aagaaaacua ucaagaaaaa cauguuaggc guccuacuuu ucaaugaagg ucaaacagca      180

gcagacacgc gccucaaaag aggagcaaga agacguuaca caagaaggaa aaauagauua      240

agauaccuuc aagaaauauu cgccccugca uuagcaaaag uagacccaaa cuucuuuuau      300

cgccuagaag aauccagccu aguagcagaa gauaagaaau acgacgucua uccaauauuu      360

ggaaaacgug aagaagaacu ccuauaucac gauacacaca agacaauaua ucacuuaaga      420

agugaauuag caaauaacga uagaccagcc gaccuaagac ucguauauuu agcacucgcu      480

cauauaauaa aauauagggg uaauuuccug uuagaaggag aaauagaucu aagaacaaca      540

gauauaaaua aagucuuugc ugaauuuuca gagacacuaa acgaaaacuc ggacgaaaau      600

uuaggcaaac ucgacguagc agauauauuu aaagauaaua cauuuucaaa aaccaagaaa      660

ucugaggaac uucuaaaacu uucuggcgca aagaaaaauc agcucgcuca ucaacucuuu      720

aaaaugaugg ucggaaacau gggaucuuuu aagaaagugc uaggcacgga cgaagaacau      780

aaacuaaguu uugguaaaga cacauaugaa gaugaucuca augauuuacu ugcagaggcu      840

ggagaccaau accuagacau auucgucgca gccaagaaag ucuaugaugc agcaauccua      900

gccuccaucc uugauguaaa agauacacaa acuaaaacug uuuucucaca agccaugauc      960

gagcguuacg aagaacacca aaaggauuua auugagcuaa aacgaguauu caagaaauau     1020

cuacccgaaa aaugccacga cuucuuuagc gagcccaaaa uaagcggaua cgcugguuac     1080

auagacggaa aaguauccga agaagauuuc uacaaauaca ccaagaaaac auuaaaaggg     1140

aucccugaaa ccgaagaaau ccuccagaaa auagaugcaa acaauuaucu ucgcaaacaa     1200

cgaaccuucg acaacggagc aauaccacau caaguacacc uuaaagagcu aguugccaua     1260

guggaaaacc aagggaaaua uuaucccuuc cuacgagaaa auaaagacaa auuugagaaa     1320

auauugaacu uucguauacc cuacuaugua ggaccacuag cgagagguaa uucaaaauuu     1380

gcuuggcuaa cccgagccgg ugaaggaaaa aucacaccau auaacuuuga cgaaaugaua     1440

gacaaagaaa ccagugcaga agauuucaua aaaagaauga ccaucaauga ccucuaucuc     1500

ccuaccgaac caguauuacc caaacauagc cuacuauaug aaagauacac cauauuuaac     1560

gaacuggcag gaguuagaua cguaaccgaa aauggagaag caaaauacuu ugacgcacaa     1620

accaaaagau caaucuucga auuauuuaaa cuagaccgua aagugucaga gaaaauggua     1680

aucaaacauu uaaaaguggu caugccugca auaagaauac aagcacuaaa aggacucgac     1740

aauggaaaau ucaacgccag uuacgguacc uacaaagacc uaguagauau gggaguagca     1800

ccagaauuac uaaacgauga aguaaacagc gaaaaauggg aagacauaau aaaaacgcua     1860

acaauauucg aaggucgaaa acuuauaaaa cgacgucuug aaaacuaccg ugauuuucua     1920

ggcgaagaca uuuuaagaaa acuaucucgc aagaaauaua caggaugggg aagacuauca     1980

gcaaaauuac uagauggaau auaugauaag aaaacacaua aaacaauacu agacugucua     2040

augacggaag auuacucaca aaacuuuaug caacuaauaa augacgacac uuauucauuc     2100

aaagaaacaa ucaaaaacgc ccaagucaua gaaaaagaag aaacacuagc uaaaaccgua     2160

caagaacucc caggaucucc ugcgauaaag aaaggaauac uacaaagccu cgaaauagua     2220

gaugaaauca uaaaaguuau gggguacaaa ccaaaaagua ucguaguuga gauggcgcgc     2280

gaaacacaga aaacacaugg uacacgaaaa cgagaagacc ggguccaaca aauaguuaaa     2340

aacuuaaaag acgcaaauga auuacccaag aaauuaccaa guaacgcuga acuuucagau     2400

gaacgcaaau auuuauacug uuuacaaaau ggccgcgaca uguacacagg ugcaccucuc     2460

gauuacgacc aucuacaauu cuacgacguc gaccacauaa uaccccaauc auuccuaaaa     2520

gacgacagca uagaaaauaa aguacucaca auuaagaaag aaaaugucag aaaaacaaac     2580

ggucucccca gugaagcagu aauacagaaa auggggagcu ucuggaagaa auuacucgac     2640

gccggagcga ugaccaacaa gaaauacgau aaucucagaa gaaaucuuca cggaggccuu     2700

aacgaaaaau ugaaagaaag auucauagaa agacaacuug ucgaaacccg ccaaauaacg     2760

aaauacguug cucaacuauu ggaucaaaga cuaaacuaug auggaaacgg uguggaguua     2820

gaugagaaaa uagcuauagu aacacuaaaa gcacaacucg caucacaauu uagauccgaa     2880

uuuaaacuaa gaaaaguacg agcauuaaau aacuuacauc augcucacga ugcguaccua     2940

aaugcugucg uagcaaaucu cauaauggcc aaauauccag aauuagaacc agaauuuguu     3000

uauggcaaau aucgaaaaac aaaauuuaaa ggacugggaa aagcaacagc caaaaauaca     3060

uuguaugcaa acguacuaua uuucuuaaaa gaaaacgaag uauauccauu cugggacaaa     3120

gcaagagauu uacccacuau uaaaagauac cuuuaccgcg cacaaguaaa caaggucaga     3180

aaagcugaaa gacaaacagg aggauuuuca gacgaaaugc uagucccuaa aagcgacagc     3240

gguaaauuau uaccccgaaa agaagggcuu gacccgguua aauauggagg cuacgcuaaa     3300

gcugucgaaa guuacgcugu auuaauaacc gccgacgagg ugaagaaagg aaaaacuaag     3360

aaaguaaaaa cucuaguaaa cauaccaaua aucgauagua agaaauauga agccgaucca     3420

accgcuuauc uagcaucaag gggcuacaca aacguaacaa auuccuucau acuucccaaa     3480

uacagcuuau uagaggaccc agagggccga agacgauauc uagcuucauu uaaagaguuu     3540

caaaaagcaa acgaacuaau acuaccccag caccugguag aacuguugua uuggguaaau     3600

gcaaaagacg gugaacaaaa acucgaagau cauaaagcag aauuuaaaga acucuuugau     3660

aaaauaaugg aauuugcaga caaauacgua guagcgccga aaaacagcga gaaaauaaga     3720

agauuauaug aagaaaacca agaugcuacg ccaauggaac uagguaagaa uuucguugaa     3780

uuacuccgau auacagccga cggugcagcc agugacuuca aauucuuugg ugaaaauaua     3840

ccuagaaaaa gguacaacuc agccggaucc uuguuaaaug gaacucuaau auaccaaucg     3900

aaaacaggau uauaugagac uagaauugac cuugguaaac uuuga                     3945


<210>  5247
<211>  3948
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5247
auggguuacu ccguaggccu ugauaucgga acagggagug uagguugggu aguaaccaau       60

gaagauggaa aacuagcucg cgccaaaggu aaaaaucuaa uuggagugcg acuauucaaa      120

acagcagaac cagcagcgga cagaagaaca aauagaacua cccgaagaag auuaagccgu      180

cgaaaauggc gacuacgacu auuagaugaa uuauucgcca augaauuagg aaaaauugau      240

ucaaacuucu uaacacgacu caaauacuca uauguucauc cuaaagacga cgcuaaccgg      300

gcaaauuacu acggaggaua cuuauuucca acccaagaag aaacaaagaa auuccacgca      360

aaauacccaa caauauacca ucuuagauua gcacucauga cagaaagcag acaauuugac      420

cuacgagaaa uuuaccuugc caugcaucaa auagugaaau aucgaggaca cuucuuaaau      480

agccagagua aaaugacaau agggaacacu uauaaccccc aggauuugca aaccgcuaug      540

gaaaacuaug cugaagcaaa aggcuuaagc auagaacuaa aacaaccaga agaaauggua      600

gcgauucuca caggugaagc uggauucggu cuaucuaaga aagcuaaagc cgaaaaacuc      660

cucaacaacu ucuucuuuga uacuaaagaa gaaaaagcaa caguucaggc uauacuagca      720

ggaaucgucg ggaacacaau ugauuuuaca aaaguauuca accgccaacu cacuggagac      780

acucuaaaag cguggaaacu aaaacuagac ucagaaacau ucgacguuga cucagaaccu      840

auacucagug aucuagauga ugacgaaacc gaacuuuucu uugcaauaag acaggcuuuu      900

gauggauuua cacuuaugaa cuuacuugga gaccaaacgu caaucuccgc cgcgauggua      960

gaacgguaca cacaacauca cgaugaucua uuaacaguaa aacaaguggc aaagaaacaa     1020

ggacucucac acaaagaguu cucaaauaua uacucagcau uccuuaaaga ugauaccgac     1080

aaagggauga agaaacuucu cgaagaauca aauuuaucca cagaagaugu aaauggcaua     1140

caauuaagaa uggauagcca ugacuuucua ccccaacaaa gaacaaaagc aaacagcgua     1200

aucccacauc aacuacacuu agcagaauua gaagccauaa uagaaaacca agggcaauac     1260

uacccauucu uauuagaaac auuugaaaaa gaaggaacag cagcaaacaa aauagcagaa     1320

cuuuuaaaau uccggauacc auauuauguc ggaccuaugg uaaccaagaa agacguagaa     1380

aaugcaggag gagaugcgac aaaccacugg gucacaagaa acgaaggaua cgaaaaauca     1440

gcuguaacgg ccuggaacuu ugaccaagua uucaauagag aucaagccgc gcuugacuuu     1500

auacagcguc uaaccgggac ugacaccuau cuaauaggcg aacccacauu accacagaac     1560

uccuuaaaau accaaauguu cacuguauua aaugaauuaa auaauguaaa aguuaaugga     1620

cgaagaauag auaaucaaac caaacaugau uuaauacaag aucuauuuaa aucuaagaaa     1680

acaguaucac uuaaagcauu ucaagauuac uaccaaucac aaggaaaaag cgacauacag     1740

guaguggguc uagccgacaa aacgaaauuu aauucaaacc uaucuaccua uauugaccua     1800

ucgaaaaccu uuagugccga agaaauggaa aauccacaaa accaagaucu ccuagaacaa     1860

auaguugaga uacagaccgu auuugaagac agcaaaauag cagaaaggga acuaagcaaa     1920

uuagaacuaa caccugaaca agugacaaaa cucgcuaaaa cacacuacac cggaugggga     1980

aaccuuucca auaaauuacu uacgaccccc auuguagacg ucagugguaa gaaagucuca     2040

auuuuagacc aacuagcaaa aacaagcaaa aacuucaugu cgauaauuuc aaaugaacaa     2100

uauggugucc aagauuggau caaagaacaa aauacgacug agaccacugg aacuuuagaa     2160

gauaaacuag ccgauuuaac uacgagccca gccaauaaac gagguauaaa acaagcauuc     2220

aacguacuuu ucgauauaca aaaagcaaug aaaucugaac cagauagagu cuaccuagag     2280

uucgcaagag aaacacagac aucagucaga acaaugagcc guuacaacua cuuaaaagga     2340

cucuaucaau caaaaucucu ugaugcagaa gcuaaaaauc ucaagaaaga acuugcagaa     2400

accgaccaaa agaaauuuga caaugaccga uuauaccuuu acuuccaaca acaagggaaa     2460

gauauguaua caggucaacc cauuaacaua gauaaacuaa guucagacua cgauauugac     2520

cacauuaucc cucaggcuuu cacuaaagac gacucaaucg auaaucgagu ucuaguaagu     2580

cgacccgaaa augcuagaaa auccgacucg ggcacauaua caacagaagu ccaagcggcg     2640

gccucaggcc uguggaaaac auuaguacaa uccggauuua ucagcagaaa gaaauacgau     2700

cgguugacuc augcugguga cuacucagaa ggacagaaaa cgggcuuuau agcacgccag     2760

cuaguagaga caagacaaau aauuaaaaau gucgcugcuc uaauagaaag ugaguucgac     2820

caaacgcaag cagucgcaau acgagcagaa auaacaggag acaugagaag acuuguagcu     2880

auaaagaaac aucgagaaau aaacuccuuu caucaugccu uugaugcacu ucuaauaaca     2940

gcggcaggac aauacaugca gaaaagauac cccgauagag acaguaauaa cguauauaac     3000

gaguucgaca gguauacgaa ugacuaucuc aagaaauuaa gagaaucauc aagcaaagac     3060

gaagugcgua aacuuaaacc auuuggauuc aucguaggaa caauggcaaa agguaacgaa     3120

gacuggacaa cagaacaaac ggaauacuua cgucauauca ugaauuuuaa aaauauacug     3180

accacauaua aaaccggacc agacagaggu ccacuaagca aagaaacuau auacgccgua     3240

gacccaaaag caaaauuaau uggaacaaac aaacaucgac aagacguauc auuauauggc     3300

ggguauaccu accccuauag cgcuuauaug acucuuguac gagcaaaugg uaaaaaccaa     3360

uuaguuaaag uuucuagauc ugccgcauca uuaaugaaau caggacaacu caaauuagaa     3420

gacuacguac aacaacaacc agaaguuaag aaauucgaga aaauauuacu agaacaucua     3480

ccucucgguc aacucguaga uaacgacggu aaauuaaugu auuuaacaag cuaugaguuc     3540

uaccacaaug caaaacaacu guggcuauca acagaaaacg cugaccuuau cucaagccuc     3600

aguauagaua gcgaugauca aaaacuaauc aaggcuuucg auauccuuac ugcagacuca     3660

gucauagaaa gauucccauu uuacgaaaua gaucugaaaa aguuaaucga auuaagagac     3720

aaauuuguac ucauagauaa caaauuagau guacugagag ucguuuuaaa agcauuacaa     3780

cuagauagug cacaacaaaa accacuaaaa cucauagaua aagcagcaac agauuggaaa     3840

aacuaucgac aagcaggagg cauaaaacuu agugauaaug cagaaaucau auaucaauca     3900

acuacaggaa uauucgaaaa gagaauuaaa aucucagauc uucuuuga                  3948


<210>  5248
<211>  3951
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5248
augcugcaaa aaaauuauac aauaggccua gacauaggca cuaacucugu aggauaugcc       60

guuauaaacg acaacuauaa aguuccagca aaaaagauga agguauuagg aaauacugac      120

aaaaaaacua uaaaaaaaaa cuuaaugggc guauuauuau ucgaugcugg agaaacagcu      180

gcaacaacaa gauuaaagag aggcacucga cggcgauaua cacgacgacg aaauagauua      240

cgguaucuac aagaaauacu aggaccugaa uuagcaaaag uugaugcaaa uuuuuuacau      300

agacuagcag aaucaucacu cguaacagaa gacaaagaau ucagcgccua cccuauauuu      360

ggagauaggg aagaagaacu uuuauaccac aaaaccuaca aaacaauuua ucaucuuaga      420

ucagcacuag caaacaagga cgaaaaagcc gaucugcgac ucauuuacuu agcucuagcc      480

cauauuauaa aauauagagg aaauuuucuc auagaaggag aaauagaacu aagcgauacc      540

gauauugaua aaguuuaugc ugauuuuugu agaagcuuca augaacuauu cgacacugag      600

guauuacaag aaauagaggu agaagccauu uucaaagaca aaacacuaag caaaacaaaa      660

aaagccgaag aguuaguaaa aguaucaggg gucaaaaaga aucaaguagc ucaucaauuu      720

cuaaaaauga ugguaggaaa cacuguuaau uucaaaaaag uauucaauuu aacggaagac      780

cagaaauuau cuuuuggaac agacucauau gaagaugagu uaaaugauuu auuagccaaa      840

uccgaugauc gauauuugga cguuuucguu gcugcaaaaa agguauauga uacugcaaua      900

uuagccaaua uucuuaauac aaaaaacagc guggcaacaa aaacaauauu cagccaaagu      960

augauagaaa gauuuaaaga ucaccaaaag gaccuaaaag aacuuaaaca auuguuuaaa     1020

acuuaucuac cagaaaaaua cccagauuuc uuuggaaauu cuaaagaaga uggcuacgcu     1080

ggcuacauag aaggaaagac aaaugaagaa aaauucuaua cccacacaaa aaaaauucuc     1140

aaaaauaucc cacaagcugc aaguguauua gcucaaauug acgucggaaa cuaccuacga     1200

aaacaaagga cauuugauaa cggaguaaua ccacaccaaa uacaacuaaa agaacuucua     1260

gcaauaauug aaaaucaagg caaauacuac ccuuuccugc ucgaaaacaa agaaaagauu     1320

gccaaaauac ucacauuccg uauuccauac uauguagguc cacuggccag gggaaauucc     1380

agauuugccu ggcucaaacg uguaggaaaa gaaaaaauaa caccauuuaa uuucgaaaau     1440

guuguugacc ucgaaucaag cgcggaacgc uucauagaaa gaaugacuuu aaaugaccua     1500

uauuuaccag aagaaaaagu acuccccaaa cauagcaugu uauaugaaaa auuuucaaua     1560

uucaacgaau uagcaggaau ucgauacaua acggacaaug gagaaaugaa auuccuggac     1620

gcacaaacua agcaaucgau auucgagcuu uuuaaacaag agaggacagu aaaggaaaaa     1680

aagguauuag auuuacuaaa aacgcucaug ccuagcgaac gcguaguugc aauuaaaggu     1740

auagacaacg gaaaauuuaa cgcaaguuuu ggaacauacc acgauuuauc gaagaugggg     1800

uucaaaacag aaaaaauagc uagcgagucu aaccaagccc gcugggagga aaucauaaag     1860

acgcuaacaa ucuuugaaga cagaaaacua auuaaaucgc gacugcgaaa auaugaugau     1920

uuccugaaug aaaaagaaau cggagcauug ucaaaacgca aauacacagg auggggacga     1980

uuaagugcua agcucaucga cggaauauau gacaaaaaaa cacaucaaac aauacuagac     2040

uuucuuauga augaagauua caaucagaau uucaugcaac ucauaucaaa cgauaauuau     2100

ucauucaaaa aaauaaucga agauucacaa guuaucaaag aagaaggauc gcucacagaa     2160

guaauacaag aauuaccugg aucaccagca auaaagaagg gaauucuaca aagcauagaa     2220

aucguagacg aguuagucaa aguaaugggc ggaaauccaa aaaaaauagu uguagaaaug     2280

gcgagagaaa cccaaaaaac acacgggacu acacgcagag aagcaagaau uaaagaaaua     2340

aucaagaacc ucaauaaacu aaaguuaaac gaacuuccaa aagaccuccc aucaaaugag     2400

gagcuaucug acgaaaaagu auaucuauau ugccugcaaa auggcagaga uauguacacu     2460

ggagcaccac uugaccuaga uaaccuguca caauaugaag ucgaccacau aauaccccaa     2520

ucauuucuaa aagaugacuc uauugaaaac aaagucuuga cagcaaaaau agaaaauagu     2580

agaaaaacaa acggacuacc aaguguagaa auagcgaaaa aaaugggagc cuucuggagg     2640

agcuuacuaa aaguaggagc aauaucugag aaaaaauaca gaaaucuaag acgaucucua     2700

cacggaggaa ugacagaaaa auuaaaggaa acauuuguaa aaagacaauu agucgaaacu     2760

cggcaaauca caaaauaugu cgcacaaaua cuaaauucac gguucaauga agagaaaaac     2820

gaagacaagg uugccaucgu gacacuaaaa gcccaacuaa caucucaguu cagaaaggau     2880

uuugaacugu auaaaguacg cgagaucaau aaucuacacc acgcacacga ugcuuaccua     2940

aacgccguuu uagcaacauu acugaugacc ucauuccccg agcuagaacc agaauuugua     3000

uaugggaaau acagaaaaac aaaauuuaaa ggguugggaa aagcaacagc aaaaaauaau     3060

uuauacacga auauacucca acugcuaaag gaagaaucug cccaauauug ggauacaaaa     3120

gagcgcgauc uagcaacaau uaaaaaaaau gucuaucaag cccaaguaaa caaaguacgg     3180

aaaguagaaa gacaaacagg cagcuuuuau gaugaaaccg uagcaaaaaa aucagcaucu     3240

caaaaacuau ucccuagaaa gaacgggcua gauccaguua aauauggagg guaccaacgu     3300

gcuaaugaag gauauagucu gcuguuuaga gcagauaugc uaaacaagcg agguaagaca     3360

aagucaguaa aaacguuagu gaagauacca auaauugaaa gugcucaaua ucuggcagau     3420

ccuacagaau aucuggccaa acgcggcuau uacaauguuu cagaacauuc gcucuuacua     3480

ccuaaauucu cucucuugga aaacaacaau ggacucagaa gauaccuagc uagcucaaau     3540

gaauuccaaa aagcaaacga acuaaucuua ccaaaacacc uagucagacu auuauacugg     3600

ucacaaucaa agcaaagaaa ggaagaacua aacaaacacc gcgcagaauu ugcugaauua     3660

uucacuuaua ucauggacuu ugcugauaaa uacguaguag cacccaaaaa cagugaaaaa     3720

auuaaaaaca ucuacgaaaa caauaaagac ucaaguguag aaguacuggg aaaaaacuuc     3780

auagaacuau uaaaauauac ugcagacggu gcagccagcg auuucaccuu cuucgacaca     3840

aaaauaccca gaaaaagaua uaauucugca ggugacuuau uagauggaac ucuaaucuau     3900

cagagcaaaa caggauuaua cgaaacacgg auagauuugg gggcaauaug a              3951


<210>  5249
<211>  3957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5249
augaugauaa aacuuaacaa caugaagaaa auucuaggac uagacuuagg aaccaauucg       60

auuggauggg caguaguuga gacggaagau aacaagaaau uugaacuacu ggacaaagga      120

guacgcauau uccaggaggg cgugaaaaua gaaaaaggaa uagagagcag caaagcagca      180

gaaagaaccg gauaccgaag cgcaaggaga auaaaauucc gcagaagacu ucgaaaaauc      240

gaaacacuaa aaguauugau agaaaacaau augugccccc ucucaaugga ugaacuuauc      300

gucuggaaag aaaacaaaaa caacuacccc aaaaauaaaa uauuuuacaa uuggcuaaaa      360

acaaaagacc ccaaagaagg aacuaauggu accuacauaa acccuuacua cuucagaaca      420

gaagcaauaa aacggaaauu agaaaacacg aacgauguag guagagcacu auaucaccuc      480

gcacaacgcc gaggcuuuaa aaguaauagg uuggaaagca cagacgaaaa aucaagaggu      540

auaguuaaga cugaaauaaa caaacuuagc ucggaauuaa acggacgaua cuuaggagaa      600

uacuuuuacg aacuauaccu gaagggugaa aagaucagaa aacgacauau aaguagagag      660

gaacauuauu uaaaugaguu caagaaaaua ugugaagugc aaaauuuuaa caaagaacuu      720

gcagaaaaau uagaaagagc aauauucuac caacgaccgu uaaaaucgca aaaaggacug      780

guagggaaau gcacguuaca aaauaacaaa caccgcuguc caauaucgcg accagaguuc      840

gaagaauuua gaauguggca auucauaaau ucaauaaaau auaaaaacuc agacgguaaa      900

uucgacuucu uaaauaaaga acagagagaa aacauaauac cagcuuucgu caguaauaaa      960

aacgccacgu uugaauuuuc aaaaauagag aaaauccuca auaaaggcgu ccacauauac     1020

caauuuaauu auaaauccaa ucauacaaua ggaucgaacc aaacaaugau aaauuuuaug     1080

gaugucuuug acauagauag gaaacugacc aacgcauggc uccuaugcgu aaacaaaauu     1140

uacgaacuau auaccgcgaa gacaaagaag gauggaagcc uaaaauccaa gagugaaguc     1200

auaacugaug uauggcaugu acuauucacc uucgauaacg aagaaaacuu aaaagaguuc     1260

gcaaggaaua aauuacuacu agaugaauca agcgcgcaga aauuuucaaa aaucaaucuc     1320

aaacaagacu auagucaacu uucccuaaaa gcgauuaaua aaauuuuacc auaucuacaa     1380

gaaggucuua uauacaguua cgccgucuuu auugccaaca uagaaaaugu gauaggcaaa     1440

ucaauaugga acauuaagga aaaccaacaa auaaucagaa cuuccaucaa aaagauaaua     1500

gacaaccaua aaacagaauu agauauaaac acgaucacaa acucauugau aaacgacuuc     1560

aaagucaauu acaauaacgc gcacaaagaa uauaaagucg acgacauaga uuuaaagaac     1620

auccgcgaga aaauaauaaa auacuauggg aacaaaacuu ggaacugucu accagaagac     1680

gaaaaacuau augugaaaga aaaaguagaa aaacuauaug aaaaacaacu aaaaaguggu     1740

aaaagaggaa uauucauaga gaaaauacga uuggaugauc ggauagaaaa uuuccugaaa     1800

gacaauuuug acagcucaga acuuaacuua aagaaacuau accauccauc ugauguauca     1860

guauacaaaa gugucaaaau aaccgacuua ggucuacgac uauugggauc cccaaugaua     1920

ccuucaauua aaaaucccau ggcuaugagg gcaaugcacc aacuaagaaa acuaauuaau     1980

caucucauaa uagaaggugu caucgacgau agcacuauaa uaaaugucga aaugucucgg     2040

gaauuaaaug acgcaaacau gagaaaggcg auagaaagau accagagaca acgggcaaca     2100

gaaaaugaag aauuuaagac aaaaauaaaa gaacucuaua agaaagaaug cgguacugac     2160

uucgaaccca acaacgacga caucaugaaa uacagacuau gggaagaaca aaaccacaaa     2220

uguuuauaua caggaaaauc aauagguauc ugcgauuuua ucggaucuaa cccagcuuuc     2280

gaaauagaac auacuauucc aagaagucua aguuacgaca acagccugga aaacaaaacu     2340

cuaugcgaaa aagaauuuaa ccaacaagua aagaaaaauc gaauacccuu cgaauuaagu     2400

aauuauaaag aaaucauaag uaggauaaau aauugggaaa uaaaagcgga agaauuguuc     2460

aaacaaauac aaaaagugaa aauucaguca cgcauggcag ccacuaaaga aauaaaagac     2520

aacgcaaucc aaaggaaaca ccuacuacaa auggaguuca auuauuggaa uggaaaacua     2580

ucacgauuca caagagaaga ugucccggaa ggauucaaaa acagucaacu uguagauaua     2640

gguaucauaa caaaauacgc uagggcauac uuaaaaaccg uauucaacaa cguaaacacg     2700

guaaaaggaa gcacgguugc ugaguucagg aacaucuggg gucuucaaga auauuucgag     2760

aaaaaggaua gaaguaauca uauacaccac ugcauugaug caauaacuau cgcaugcuua     2820

acuaaagaua aauaugacuu cuuagcacac cucuaucauc uggacgaaca gaacaaccga     2880

gacgcagucc acagagcuau gaucaaauca aaacccuggg aaaacuucgu acaggaugug     2940

aaagaaauag aaaaccaaau ccuagucagc cauuacacac cggauaaccu accaaaacag     3000

ucaaagaaaa uacuucgaaa aagaggaaaa auccagaaaa acgaaaaugg agaucuaaua     3060

uacgaaaaag gcgacgcagu ucgagggagu uuacacaaag aaucauuuua cggagcuaua     3120

aaacgugaaa uaauaaauaa gaaaggcgaa acagaagaaa aaguacugua uguaaaaagg     3180

aaaccacucg aauuuagcga ccuaggauuc aagacaauau cagaacucga aaacauagua     3240

gaugacgcag uaagagauaa aguuuuaaac gcaguaaaag acaaaggauu aaaacaagca     3300

cuaaacgaac caauauggau gaaugaagaa aaacaaauac cuauaaagaa aguucgaugc     3360

uacacaccau caguaaccaa ccccaucaau cucaagaaac aucgagacaa aagcaaacau     3420

gaauauaaac aaucauacca uguuacaaac gauggcaacu acuuaauggu gauauaugaa     3480

ggaaaagaca gccaagaaaa aguaaaacga gacuaugaaa uaguaaguaa cuuacaagca     3540

ggagaauauu uuaaacaauc aguacacggu gucuuaaaag cacaaggaau aaacaaucua     3600

gaaggacuaa uaccaaaauu caaagaauca gguaaaaaua uacuaccucu caaauacaua     3660

cuaaaaacag gaaccauggu cauauuuugg gaaaauuauc cagaagaaau augggauauu     3720

gauaaaaacc aauuauacaa gcgacuguuc aaaguaacac aguuugaauc agacgggcgu     3780

auacaagcaa gauuucauca gaucgcaaaa ccggacaacg aacuaacuaa aguuagugaa     3840

uuagacuuuu ccaacauagc agaaaagaua agacuaucaa aaggaaauuu aaacaugcug     3900

gugaauggau augacuuuaa gaucaacgua cuagggcaca uagaaaaguu aaguuga        3957


<210>  5250
<211>  3957
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5250
augggaucua acaugcuuaa aaaagauuac agcaucggcc uugauaucgg gacuaacucg       60

guaggacaug ccguagucac agacgacuau aaagucccua cuaaaaaaau gaaaguguuc      120

ggagacacgu caaaaaaaac aauaaaaaaa aacauguuag gaguuuugcu auucaacgaa      180

ggucaaacag cggcagacac aaggcucaaa agaggaacac gaagaaggua uaccaggcga      240

aaaaauagac uucgauaccu ccaagaaaua uuugcaccag aacucgcaaa agucgaccca      300

aacuuuuuau acagauuaga agaaucaagc uuaguagcag aagacaagaa auaugacgua      360

uacccaauuu ucgguaaaag agaagaagaa cuauuauacc acgauacaua caaaacaaua      420

uaccacuuaa gaucugcgcu agcaaacaac gaccagccag cugaccuaag acuuguuuau      480

cuagcuuuag cucauauuau aaaauacaga ggaaacuuuu uauuagaagg ugaaauugac      540

cuaagaacua cagacauuaa uaaaguuuuc gcagaauucu cagaaacuuu aaacgaaaac      600

ucagacgaaa acuuaggaaa auuagauguc gcggacauau ucaaaaacaa cacuuuuuca      660

aaaacaaaga agagcgaaga acucuuaaag uuaucaggag cuaagaaaaa ucagcuagcg      720

caucagcucu uuaaaaugau ggucggaaac augggcucau ucaaaaaagu acuaggaacg      780

gaugaagagc acaaacuauc cuuuggaaaa gacacauacg aggacgaccu uaaugaccua      840

uuagcagaag caggagacca auaucuagau auuuucguag ccgcaaaaaa aguauacgac      900

gcagcgauuu uagccaguau acuagauguu aaagacaccc aaacuaagac gguauuuagu      960

caagccauga ucgaaagaua cgaagagcac caaaaagacu ugaucgaguu aaaacgagua     1020

uuuaaaaaau auuuaccaga aaaaugccac gauuuuuuua gcgaaccaaa gauaaguggc     1080

uaugcagggu auaucgacgg aaaaguaagc gaagaagauu ucuacaaaua uacuaaaaaa     1140

acauuaaaag gcauucccga aacagaagaa auacuacaaa aaauagaugc aaauaauuac     1200

cuaagaaagc aacgaacauu ugacaauggc gcuauaccac aucaaguuca ucuaaaggaa     1260

cuaguagcua uuguagaaaa ccaaggcaag uauuauccau uccuucgaga gaauaaagau     1320

aaguucgaaa agauauugaa cuuucgcaua ccguauuacg uuggaccauu agcccgcgga     1380

aacucaaaau ucgcauggcu cacccgagcu ggugagggaa agauaacgcc auauaauuuu     1440

gaugaaauga uagacaaaga aacuucagcc gaagauuuua uaaaacauau gaccauaaac     1500

gauuuauacc uacccacaga acccguacuc ccuaaacacu cacuuuuaua cgaaagauau     1560

acaauauuua acgaacuagc uggcguaagg uacgucacag aaaacggcga agcaaaauac     1620

uucgaugcac agacuaaacg aaguauauuc gaguuauuca agcuagaucg aaaaguaagc     1680

gagaaaaugg ugauaaaaca ucuaaaaguu gucaugccag caauacguau acaggcccua     1740

aaaggacugg auaacggaaa auuuaaugcu ucauacggaa cauacaaaga ccucguagac     1800

augggaguag ccccugaacu auuaaaugau gaaguaaauu cagaaaaaug ggaagauauc     1860

auaaaaacgc uaacaaucuu cgaaggcaga aaauuaauaa aaagacggcu ugaaaauuac     1920

cgagauuuuc uaggagaaga cauccugaga aaauugucca ggaaaaaaua caccggaugg     1980

ggcagacuau cagcuaaacu auuagacgga auauacgaua aaaaaaccca caagacaaua     2040

uuagauuguc uaaugacaga ggacuacucg caaaacuuca ugcaguuaau caacgacgau     2100

aacuacagcu ucaaagaaac uauaaaaaac gcccaaguca uagaaaaaga agaaacguua     2160

gccaaaaccg uccaagaacu cccaggauca ccugcaauaa agaaaggaau ucuacaaucu     2220

uuagaaaucg uugaugaaau aauuaagguu augggguaua aaccaaaaag cauaguagua     2280

gaaauggcuc gagaaacaca aaaaacccau ggcacccgaa aaagggaaga uagaguccaa     2340

caaauaguag agaacuuaaa agaugcuaau gaacuuccag aaaaauuacc aucaaacgcc     2400

gaacuaagcg augaaagaaa auaucuuuac ugcuugcaaa augggaggga uauguacaca     2460

ggagcaccac uggacuacga ucaccuccaa uuuuacgaug uagaucacau aaucccgcag     2520

ucauucuuaa aagacgacuc gauugaaaac aaaguccuaa cuauaaaaaa ggaaaacgug     2580

agaaaaacua auggcuuacc cucagaagcg gugauucaaa aaaugggauc auucuggaaa     2640

aaguuacuag acgcuggugc aaugacgaau aaaaaauacg auaaccucag aagaaacuua     2700

cauggaggac uaaacgaaaa auuaaaagaa cgcuuuauag aaagacaauu aguagaaacu     2760

cggcaaauca ccaaauacgu agcgcaauua cuugaccaaa gauugaacua cgauggcaau     2820

ggaguagaau uagaugaaaa aauugcaaua gugacgcuaa aagcacaacu agcuucacag     2880

uucaggagug aauucaaacu uagaaaagua agagcauuaa acaacuuaca ccaugcacau     2940

gacgccuacc uaaaugcaau cguagcaaac cuaauaaugg caaaauaccc agaacuagaa     3000

ccagaauucg uauauggaaa auacaggaaa gcaaaauuca aagggcucgg caaagcaacc     3060

gcgaaaaaua cccuauaugc aaauguucuu uacuuucuaa aagagaauga aguauaucca     3120

uucugggaua aagcaagaga ucuuccaacc auuaaacgau accucuaucg agcacaagua     3180

aauaaaguaa gaaaagcaga aagacagauc gguggauuuu cagacgaaau gcuaguucca     3240

aaaucagacu caggcaaguu acuacccaga aaagaaggau uagacccugu aaaauacgga     3300

ggguacgcua aagccguuga aucuuaugca guucucauaa cagcagauga aguuaaaaaa     3360

gguaaaacaa aaaaagugaa aacgcuagua aacauuccaa uaaucgauuc caaaaaauac     3420

gaagcugacc caacagcaua ucuagcaucg cgaggguaca cuaacgugac aaacaguuuu     3480

auccuaccaa aauacucucu acuagaggac ccagaaggua gaagaaggua ccuggcaagc     3540

uuuaaagaau uucaaaaagc aaaugaguua auacuaccuc aacaucuugu cgaauugcua     3600

uacuggguca acgcuaaaga cggugaacaa aagcuagagg aucacaaagc agaauucaaa     3660

gaauuauuug acaaaauuau ggaauuugcu gacaaauacg ucgucgcacc uaaaaacagc     3720

gaaaaaauuc gacgacuaua ugaagaaaau caagacgcaa cuccaaugga auuaggcaaa     3780

aacuucguag aacuccuaag auacacagcu gauggggcag caucggauuu uaaauuuuuc     3840

ggagaaaaua uuccgagaaa aagguacaac ucagcgggua gucuacuuaa cggaacacua     3900

auuuaccaau ccaaaaccgg acuauacgaa acacgaauug acuuagguaa acuuuga        3957


<210>  5251
<211>  3960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5251
auguuuaguc aaagaaacaa cccauacugc gaauuauuau ucaaaauuau aguaaccaau       60

auagaagaga aaaauuuaga agucauuaug gguauaagaa acucagauau auuuuucauu      120

agcaauuuua ugugguacca aaaauuaaua augaugauca aaaauauacu aggacuagac      180

uuagggacaa auaguaucgg augggcacua auagaaguug acgaaaacaa cauaccuaua      240

agaauaauug cuaugggauc uagaauaaua ccacuaucga caagcgacaa agaagaguuc      300

caaagggggu uauccauuac gaaaaaccag aacaggacag ucagcagaac acaaaggaaa      360

ggauacgaca ggaaacaacu acgaagaucg gaccuaaaga aaauauuaaa gaaauacaac      420

auauucccga gugaagaacu auuaaaauua ccaauguugg aucucuggaa acuacgcuca      480

gacuccgcua acccaaacga agacauaagu gcugaacaau uaggacguau cuuauauaug      540

cucaaucaga aacgaggaua caaaucagcu aggucagaag caaacagcga caagaaagau      600

acagauuacg uacaagcagu aaaagguaga uaugcacaac uuaaagauag gagacaaaca      660

auaggacaau acuuuuauga agaauuguca acagcauucu cagacaaaaa uaaccaacaa      720

uacuuucgug ucaaagaaga aguauaccca agagaagcau auauagaaga auuugauaca      780

auaaucaacg cacaaaaaga uaaacaugga uuccuuacag acaaaguaau acaccaacuc      840

agaaacgaaa ucaucuauuu ucagcguaaa cucaaaaguc agaaaggguu gguuaguauc      900

ugcgaacuag aaggauuuga aaagacagua uaugauaaag agaaaaacaa aaauaaagua      960

guuuucguag gaccaaaagu agcgccaaaa acuagccccu uguuucaauu gugccggauc     1020

ugggaagucg uuaauacaau cagucuaaaa aucaaaaacc cagaaggcag caaauauaaa     1080

uggggagaaa aaguacccaa ccucaaacag aaagaaauuc uagcggacua uuuauucaaa     1140

aaugaaaauu uaucuuucuc aacacuuuua gaaauauuag aacuaaagaa agaagaugua     1200

uaugucaaca aacaaauucu aaaaggaaua aaagguaacg aggcauaugc agcucuacac     1260

aacguacucg gagaccauga auuauuaaac uucgacgucu caauaauuac gagugaccau     1320

accgcuaucc uaguagauaa gaaaacaggu gaaauauuag aagaacgggc aggacuagaa     1380

cuagacgcaa gccuagaaag agaacccuua uaccaauucu ggcacacaau cuauaguuug     1440

aaagaucuag acgaauguaa aaaugcccuu auaaaacgau uuggauugga cgaggauaua     1500

gcugaaaaau uaucacacau agacuucaau aaacaagcau ucggaucaaa aagcaacaaa     1560

uccaugagaa aaauacuucc cuuucuaaug gagggcuaca acuauagcga gucauguuca     1620

uuagccggau auaaccacuc caauucgcua acaaaagacg aaagagaauc acaaauaacg     1680

cuagaacgac uagaacuacu acccaaaaac aaccuucgac agcccauugu ugagaaaaua     1740

cuuaaucaaa ugauaaacgu aguuaauaca auuuuagaaa aauacggcaa accuucagaa     1800

auacgagucg aacuugcaag agaacuuaaa caaaguaaag acgaacgaaa ugacacagau     1860

cuacaaaauu caaggaacaa gaaacuaaau gaagaaauug gaaaaaggcu cgcagaauua     1920

ggacugccag ugacgaaaag guacauacaa aaauacaaau uuauauuucc aucccuuagc     1980

aaaaaccuca aagacgcuca cguaaacaac caauguauau auugcggcga aacuuuuaau     2040

cuaaccgaag cguuaaaugg agacgcauau gacguugacc auauagugcc uaaagcccua     2100

cuauuugaug acucacaaac aaacaaagua cucguacaua gaaaauguaa cucuacaaaa     2160

acaaaccaaa cagccuauga uuacauaacu gcuaaaggug aagaagaagu ccgcauauau     2220

agcgagagag uagacgauug guucaaaaag ggaauuauau ccuauucuaa aaugcaacgg     2280

cuaaaaguau cacacaaaga auacuuagaa agaaagaaau uaggaaaaga aaccgaaacu     2340

gauaagaaau uaugggaaaa uuucauagau cgacaacuuc gugacacaca auauauuuca     2400

cguaaaucac gugaaauucu ccaaaaagug ugcaacaacg uaacaacuac ugaaggagga     2460

guaacugcga aacuacgcaa ucuaugggga ugggacgaua uacuaaugaa uuuacaaaug     2520

cccaaauaua aagaauuggg acaaacaauc acaaaagaau ggacauccga acacggaaaa     2580

cguaagcauc aaaaagaaga aauugaaaac uggacgaaga gagaugacca cagacaucac     2640

gcaauagaug cccuaguagu agcauguaca aagcaaggau ucauacaaag gauaaacaca     2700

cuaaauucuu cagagaccaa agaucucaug auaaaagaag uagaagaggc uaaaaucgaa     2760

uuuaacgaga aaaccacauu acuagaaaaa uaucucaaaa cacaaaaacc auuuacgacc     2820

agagagguga ugaaagaagc agacaagauc cuuauaucuu ucaaagcagg caagaaagua     2880

gccacaauaa caaaauucaa agcaacaggc aaaaacaagg aaacaggagu aauagugccc     2940

cgaggagcuc uacaugaaca aucagucuau ggaaaaauaa aaguaauaga gaaagacaaa     3000

ccccugaaau accuauuuga aaauccggau aaaauaguua acucgcgaau acgugaccuc     3060

guggaaacac gccuaucaga aaacgaaaau aacagcaaga aagcauuaag uacagucaag     3120

aaaaauccaa uauucuuuaa ugaagagaaa aacgaaaucc ucgaaaaagc auccuguuac     3180

aacgacgcca cuguacuaaa auauaagcua caaaaccuaa aagccaauca aguggaugau     3240

auagucgacg aaagaguaaa auuucuugua aaagaaaggu ugacacaauu cggcagcaaa     3300

gaaaaagagg cauucaaaga cauacuaugg uuuaaccagg agaaacaaau cccaauaauc     3360

acaguccgac ucuuugcacg acccgaugcc aacaaccucc agauaauuaa aaaggauaaa     3420

aacggcaaag acaucggauu cguacuaacu gguaacaauc accacauagc aauauacgaa     3480

gacaaagacu caaagcuagu ucaacacuca ugcgccuucu ggcacgcugu agagaggaag     3540

aaauauaaca uucccgcagu uauuaaaaac agcgcagacg uuuggucccu acuacuagau     3600

aaagaacucc caguacucuu cuuagacaaa cuucccgcag augaauuaaa uuuaaaauuc     3660

ucaaugcaac aaaacgaaau guucauacuu ggucuauccc aagaaggcuu ugaugaagca     3720

aucaaaaaca augacaaaag ccuccuaagc aaacaucuau accuaguaug gucaauuuca     3780

aacaaugacu acuauuucag gcaucaucuu gaaaccaaaa acacagagcu caaaaauaua     3840

ccaacagcaa aagauaguaa acgauacuuc agauucaaau cggucggcgc cuuaguaggc     3900

auuaacccca uaaaaguuag acuuaaucac uuaggcgaaa uaaccaaaau aggugaauga     3960


<210>  5252
<211>  3960
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5252
augggauaca acaucggauu agauauaggg acuggaucag uaggaugggc cgcacuuacc       60

gacgaaggca aacuugcucg agcaaaagga aagaaucuaa uagguguacg ucuauucgac      120

ucagcacaau cagcugcgca aagaagaucg uauagaacaa caagacgccg uuuaagcaga      180

agaaaaugga gguugagacu acucgaaaau aucuuuuccg acgaaauggg uaugauagau      240

gaaaauuuuu uugcaagacu aaaauauuca uauguucacc caaaagauga agucaauaac      300

gcacacuauu auggaggaua ccuuuuucca acacaacagg aaacacauga uuuccaugaa      360

aaauuucaaa caauuuauca ucuacgacua aaacuaauga ucgaagauug uaaauuugac      420

cucagagaaa uauacuuagc aaugcaucau auugucaaau aucgaggaca cuuccuaaau      480

agccaaagca aaaugaccau aggcgacucc uacaacccaa gagacuucca acaagcuauu      540

caaaauuaug ccgaagcaaa aggauuaaua uggagccuga acgacgcaca agagaugaca      600

gacguauuag uaggacaagc aggauuuggu cuaagcaaaa aagccaaggc cgaaagacua      660

cuaucugcau uuuccuuuga uacuaaagaa gacaagaaag caauacaagc aauacuugca      720

ggcauaguug gaaacacaac agacuucaca aaaauuuuua accgugaacg aucgggagac      780

gaacucaaaa aguggaaacu uaaacuugau agcgaagcau ucgaugaaca aucucaggca      840

auuguagacg aauuagauga ugacgaaaug gaauuguuua acgcuauaag acaggcuuuu      900

gauggauuca cauuaaugga ucuccuaggu gaucaaacaa guaucagcgc agcaaugguu      960

aaacgcuauc agcagcauca ugacgacuua aagaugguua aagaaauagc aaaaaaacaa     1020

ggauuaucuc aucaagacuu uagcaaaauc uacaccgcau uuuuaaaaga cgacaccgac     1080

aaaggcauga aagcauuguu agauaaggca gacuuagcug augauguccu gguugaaauu     1140

caacaacgaa uagaaucaca ugacuucuug ccaaaacaaa gaacgaaagc gaacucuguu     1200

aucccuuauc aacuacaucu agcagaacua gagaagauua uagaaaauca aggaaaauau     1260

uauccauuuu uguuggacac auuuaccaau aaagcaggcg aaacaauaaa caaacuaguu     1320

gaauuaguga aauucagagu gccuuauuac guagggccua ugguaaccgc ggcagacguu     1380

gaaaaagcag guggagacgc cacuaaucau ugggucaagc guaacgaagg auaugaaaaa     1440

aguccuguua caccguggaa uuuugaucaa guauucaacc gagaccaagc agcacaagau     1500

uuuauagaca gauuaacagg cacagacacc uacuuaauag gcgaacccac ccuacuaaaa     1560

aauucuuuaa aauaccaacu auuuacaguc cuaaacgaau ugaauaacgu aaaaaucaau     1620

gggcacaaaa uagaugagaa gacaaagcac guguuaauac aagaccucuu caaaagcaaa     1680

aaaacuguau cagaaaaagc gaucaaagac uauuaucuau cccaagguau gggugaaaua     1740

caaaucguug gucucgcuga uaaaacaaaa uucaacucaa accuuucauc auauauagac     1800

cuuuccaaga cauuugaugc ugaauuuaug gagaacccag ccaaccaaga acuacuugaa     1860

aacauaauac aaauacaaac gguauuugag gauguaaaaa uugcagaaag agaauuacag     1920

aaacuagcuu uaccggacga acaaguucaa caauuagcaa aaacucacua caccggaugg     1980

ggcaaucuau cagacaaacu acuaagcacc ccuaucauac aagaagguuc acaaaaagug     2040

aguauauuaa auaagcuaca aaccaccagu aaaaacuuca ugucaauaau uacggacaac     2100

aaauuuggug uucaacaaug gauacaagaa caaaacacag cagaaacagc ugacucaauu     2160

caagacagaa uagacgaauu gaccacugcu cccgcaaaca aacgaggaau uaaacaagca     2220

uucaacgucc ucuucgacau acaaaaggcu augggcgaag aacccaacag aguauaccuu     2280

gaauuugcga aggaaacaca aaauuccguc agaacaaauu cacgauauaa cagguuaaaa     2340

gaccuauaua aaucuaaaac ucuauccgau gaugugaaag cauuaaaaga agaacuagaa     2400

ucacaaaaau ccagcuuaca aucggaacga auaggagaca gauuauaucu auacuuccua     2460

caacaaggaa aagacaugua cacaggacaa ccaaucaaua uagauaaacu cagcacagau     2520

uaugauauag accauaucau accacaagca uauacaaaag acgacuccau cgauaaucgu     2580

guuuugguau cacgaccuga aaaugcacga aaaucagacu cggcuacuua cacaacugaa     2640

guucaacaaa gugccggagg auuauggaaa uccuuaaaaa acgccggauu cauaagucaa     2700

aaaaaauacg auagauuaac uaaagggggu gacuacagca aaggacaaaa aacaggauuu     2760

auagcacguc aacuaguaga aacucgacaa auaauaaaaa auguagcauc acuaauugaa     2820

agugaauucu cacaaacuaa agcugucgca auaaggucag aaaucacagc ugacaugaga     2880

cggcuagucg ccauaaaaaa acacagagaa aucaauagcu uccaccacgc auucgaugca     2940

uuacuaauca cugccgcggg ccaauacaug caagcacgau acccagacag ggacggagcu     3000

aacguauaua acgaguuuga uuauuauaca aacacauacc uaaaagaacu cagacagucc     3060

aguucaagcu cacaaguacg aagauuaaaa ccauucggau uuguuguagg caccauggcc     3120

aaaggaaaug aaaauuggag ugaagacgau acacaauacc uaagacacgu aaugaacuuc     3180

aaaaauauuc uaacuacacg aaggaacgac aaagacaacg gcgcacugaa caaggaaacc     3240

auauaugcag uggacccaaa agcgaaacua auagguacca auaaaaaacg acaagaugua     3300

ucacucuaug gaggauauau auacccguac agcgccuaca ugacacuagu cagagcaaau     3360

ggaaaaaacc uucucguaaa aguaacgaua ucagcugcug aaaaaauaaa aucuggccaa     3420

aucgagcuaa gcgaauacgu acagcaaaga ccagaaguaa aaaaauucga aaaaauauua     3480

aucaacaaau uagcaauagg ucaauuaguc aauaacgacg gaaacuuaau auaccuaacu     3540

ucguacgaau uuuaucauaa cgccaaacaa uuaugguuac caacagagga agcugaucua     3600

auaagucaau uaaauaaaga uucaucagau gaagaucuaa uaaaaggauu cgacauacua     3660

acuucucccg cuauucuuaa aagguuuccc uuuuaugaau uagaucuaaa aaagcuagua     3720

aacauaagag auaaauuuau agcaguagaa aauaaauuug auauucucau gguaauauua     3780

aaagcucuac aauuagaugc agcacaacaa aaacccguaa aaaugauuga uaaaaaaagc     3840

gccgacugga aagacuacag acagagaggc ggaauuaaac uauccgauac aucggagaua     3900

auauaucaau cuacaacagg cauuuucgaa aaaaggguca aaauaaguaa ccuacuauga     3960


<210>  5253
<211>  3963
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5253
augaccgaac aacaacgacc guauaacaua ggacuagaca uaggaacauc aucaauaggc       60

ugggcgguga cagaccaaaa ugauaaauua cuacauauuc gaggacacaa uggcauagga      120

gucagacuuu ucaaagaagg agaaucagcc gcagaacguc gaggcuucag aacaacuaga      180

agaagguuau caagaagaag auggagauua cgauuucuag accgaaucuu ugacgccgaa      240

cuagccaaag uggaucccaa cuuccucgcu agauuaaagc aauccaauau aucuccacua      300

gacccaaaca aacaaauguu cggaaacauc uuauuugacg augcccaauu cgacgaucaa      360

aaauuucacc aacaauaccc aacgauauac caccuacgua gcgcacuagc acaaccagau      420

gcaccccaag cggacguacg acugauauau cuagcucuac aucacaugau caaauuucgc      480

ggccauuucu uaaauggugc cgcagucgaa aaguuccaug guggacaauu acaucuagaa      540

gccgaguucc aagaacuaaa ccaaauuuuc ucaaaccaac agagagauca cgugcaacuc      600

caaacaaguu cgcuagacga agucgcaaca auacucaaag auaaucaacg cacccccagc      660

gaccgacaaa gagaacucuc ugcaagacug uucguaccag uggacaaaac aaucgacaag      720

gaaaacaaga agcuggccac agaacuuuua aaagccguau uagggcucaa ggcaaaauuu      780

gacgucauac uaggaguaga agccacugac ccacaagcau ucaaacucca auuuucgagu      840

gaugauuuug augauaaaau agcagaacuu gcaagugaau uaacagacga agcaaaugaa      900

auccuuacag uacuacaaag auuguauuuu aguuuaaacu uggcugauau ccuuacuaac      960

aggcaaacaa aacagguugc cgacagcaua uccacagcaa ugaucaacag auaugaugau     1020

cacaaacacc aucuaaaaau gcuuaaagaa uuagaaacuc aagucacuca agaacaacga     1080

gacggauuaa agaaagcaua cgacgaauac aucgauggcg acaaaccaac aaucgauacc     1140

uuuuauacua acgucaagaa aaaccucgac gacucagaac uugcgaacca aauaaaauca     1200

uuaauagauu uacaagcauu caugccaaaa cagagaucaa aggaaaacgg aaguauaccc     1260

caccaacuac accaacgcga auuggaccaa uuaauugaag uccaaggaaa guauuaccca     1320

uggcuucuag cagcaaaaga uaaauuagac gcccuaauag cauucagggu accuuacuau     1380

guaggaccca ugauagaucc uaacgaauca gaugaccccc aaaacacuaa auucgcaugg     1440

augguccgaa aagaagccgg agaaauaacg ccauggaacu uugaggaaaa aguugacaaa     1500

acagguagug caaccaauuu cauaaagagg augacaacga ccgauacaua cuuacuugga     1560

gaaccaguau uaccgaaaaa uucccuauua uaccagcaau uuacaguuuu aaaugaacua     1620

aacaaauuaa aaguagacgg caaggcacuc acaguugacc aaaagaaaua cuuauacgaa     1680

cauguaugua agaaacaaag acgaguaacu cuaaagaaac uaucugauug ucugauggca     1740

aggggagauu uuccaauggg agcgauauuu acaggaacaa ccgaccagaa aaacaugaac     1800

aacacauuaa aaacauauca agacuuucaa gaaauauuug gugacgaaau cgauaaucca     1860

gacagacagg uagauuuuga gaaaaugauc gaguggagca ccauauucga agaucgcgaa     1920

auauuuaaac uaaaauuagc agaacuaaac ugguugacca gugaacagcu agaaagagua     1980

ucaaagaaaa gauaucaggg uuggggaaga cuaucgcaaa aauuauuaac acauauaguc     2040

aacgaaaacg gagaaaggau aauagaccaa cuauggaaua caaaucaaaa uuucauggaa     2100

auaaugggac aagauggagu aaaacaagca auucaagaac acaacggagc uaacgucgca     2160

caaaccggga uaaaugaaac aauagaugag cuauauacua gcccacaaaa uaagaaagcg     2220

auuaggcaaa ucuuauuagu cguagacgau auaaagaaag caguuggcua cgcaccagca     2280

aauauaauga uggaguucgc aagagaagau agagacgauc auagacuuau agcgucaaga     2340

gcaaggcaac uugagaaaac cuaccaagaa auaaguaaaa auugguuuga uaaugaauca     2400

guaacucaag aacuaaaaga ucagaucaaa aacaaaacaa aauucacuga cagacuauac     2460

cucuacuuca uacagggagg gauagauuua uacaccggcc acccauuaaa uauagaccag     2520

uuaagcagcu augacauaga ucauauauua ccacaaucgu uccuauuaga uaacagucua     2580

aacaaucgag uacuaacgag ucaaagcgaa aaucgugugc auaaagaaga cagacuaccc     2640

ggugaaaccg uagcaaauaa aaugcgccca accuggaaaa ugaugagaga acaaggauug     2700

augacgcagc guaaauacaa caauuuaacc cuaaaacccg aucaaaucuc aaaauucaau     2760

aaccgcggcc aauuuauaaa cagacaacuc gucgaaacca gacaaguuau aaaacuagca     2820

gccgacauua ugaaccaaaa auaccaagcu gaccaaacaa aagucguaac uguaaaagcg     2880

aacuuaacac aucacaugcg agaaaaauuu aacuucuaca aaaguaggaa uguaaacgau     2940

uaucaucaug cguucgaugc auaccuaaca guuuuuguag gaaauuggcu auuaaaagaa     3000

uauccaaaau ugcaaccaua uuucguauac ggagacuucg caaaaacggg uacaaauaac     3060

uugaaaucau ucaacuuuuu auaccgcuuu gaacgugauc auaaauuaaa ugacaaaggc     3120

gcgauagaca ccgauaaaga ucagcaauua ggguacauga aaagaauaua cagauucaag     3180

aaaaugcuag uaaccaaaca acucgaaaca aaugaaggcg cgcuauauaa acaaacaaua     3240

uacaaagcac cagcuaacga gaaaaauaca aagaaaaacc guucacuaau uaacacaaag     3300

aaaaauaggc ccucagacau auacggggga uauucacaau caguuaccgc uuauaugagu     3360

uuaguauuuu ucaaaaauac auuuaaauug auaaaaaugc cccucguagc agccaacgcc     3420

auuaaaaauu cacuaaguuc auaucaugau acaauacuga aaauucuugg aagcaaugaa     3480

uuuaaaguuu uaaucccaaa aguaaaaaug aaucaauuaa ucaaagaagg agacagucuc     3540

uacacagucg uagggguaua caaacacaau gcucagcaac ugauucuauc cgacaacuca     3600

auggguauaa ucacaaaacc uauucgugaa auaugugaca gcgaccuaga cucaguguuc     3660

aaugaacucg uugacaacau agauaaauau cuaaaccuau acaaaauaga agacaaacuc     3720

acaaucaaag aaaaauuuua ugcacuuaaa aauaaagaau ccaaauucaa auguauuagc     3780

gacaucuuag ucggcacaca cugcaacgca ggaagaagua acuuaaaaau aaucggacuu     3840

ucaaguuacu ggggaauguu cucaggaaau aacgacggau uaaaaauaaa ugaaaacagc     3900

caacuuauau accaaucacc uacuggauua uucgaacacc gaguaaaggu cucugaucuu     3960

uga                                                                   3963


<210>  5254
<211>  3972
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5254
augaaagacu ucuacauagg uuuagauaug ggaaccgacu ccgugggaug ggcuguugcu       60

gacgaagauu acaacuuaug caaauucaaa ggaaaagcua uguggggagu ucgacucuuu      120

gacgaaucag caaccgcugc agaacgaaga ggauuuagaa cggcaagacg gagagcccaa      180

aggaaaagac aaagaauauu auggcuccaa auguuauuua acgaagaaau uuccaagaaa      240

gacauagcau ucuuucaaag guuaaacgaa agcaaauugu accuagaaga caaaucuaca      300

aacguuccau acgcaguguu ugcagaugaa aguuucacag acguagauua ucacaaacuc      360

uauccaacaa uuuaccaucu uagaaaagaa uuagugcaaa gugaugaacc gcaugaugua      420

agauuaguau acuuagcccu acaccaucuu auuaaaaaua ggggacacuu uuuauuugaa      480

aacgucggaa gcgacuuuga aaaagaaagc ucauuugaag uccuauucaa aaacuuacaa      540

aacuauauau uaggagaaua cgaacuugca cuagaaugca uaucaccacu acagauagca      600

gacauccuaa aagacaaaag uaaaagaaaa acuacaaaau caagcgaaau auguaaauua      660

cuaggcauaa gcaagaaagg acaaccacga cuaaacgcua uaguccuacu gaugugcggc      720

caaagcguaa aacucgcaga uuuauacgga gacgaaaacu acaacaaaga agagaaaagu      780

ucuauaucau uugaaaaagg auaugaagac aacgaagaaa cauauagaga auuauuacuu      840

gaaagauuug aauuaaucga gaaaauaaaa gcccuauacg auugggccau cuuagccgau      900

auacugaaag gcgaaacaua uauaucauuu gcaaaaguca aaaccuauga cgaacaucga      960

caugauuuac aacaauuaaa agcauuugua aaagaacgau gucgaucaga uuacaaugaa     1020

aucuuucgug aaacauccga caaacuaaac aauuauaccg ccuauugugg uaaauucaaa     1080

gaaaauggaa gaaauggcgu cauacuacac ucagcaaauc aaacggauuu cugcaaauac     1140

cuaaaaucaa aauucgacaa acuagacaaa ucaggguaug aagacauguu ugagaaaauu     1200

gaaaacggau cauuuaugcc aaaaauagua guaaaagaca acgguguaau cccuaugcaa     1260

gucaacgaau cagaaguaag agcaauucug aaaaaugcuu caggauaccu agacuuucua     1320

aacaaucaag auaaagaugg caucacagua gccgauaaaa uucugcaaau auuugaauuu     1380

agaaucccau auuacguagg accauuaaac guacauagcg acaacucaug guuaguacga     1440

aaagauggca aaauauaccc auggaacuuc aacaaaauag ucgacguaga uaaaucagca     1500

gaagaguuca uaaacaaacu caccgcuaaa uguacuuauc ucccagacag agacguaauc     1560

cccaaaaaua guauccuaua cucuagauau augguauuaa acgaauuaaa uaaucuaaaa     1620

cuugacggaa agaaaccuga cgucucuuuc aaacaagcug uguuuaauga ccuauuuaug     1680

cauagaagaa aaguaacaca aaaggcuuua agagaauaca ucaaagcaca gaccgguaca     1740

gaaccggaaa uaacaggaau agacggagac uucaaagcaa acaugaggcc cgcgauugaa     1800

cuggcaggau acaacuuguc agaacccgac aaagaagaaa uaauaaaagc cauaaccaua     1860

uuuggagacg auaagaaacu ucuuaaacga cgaauaaaag acaaauauag ugaaaugcua     1920

ucagaagaug aaaucaagaa aauauguaaa cuuaaauaca aagauugggg acgauuaagu     1980

cgagaguucc uaacagaaau auuugacgua gauacggcua caggagaaug caaagacaac     2040

auaauaaaca cauuauggaa cacuaacgac aaccuuaugg aacuauuagg ugccaaauau     2100

aacuucaccc agucaauaga acaagcaaua ucaaaaagca cccauaaagc caguauagag     2160

aaaaugguag aggacaugua cgugagccca aaaguuaaaa gaaccguauu ccagucacua     2220

aaaauuauga aagaaauugu aaagauaaaa ggacacgcac ccaaaaaggu uuucguggaa     2280

augacaagac augaugggac uaaaggcgac aacggccgaa ccaaaaguag aaagaaacaa     2340

uuagaagaac uuuacaagaa augcaaagaa gacaguacgg aauuauggga cgaauuagag     2400

aaaacggacg augaugaguu caggcaagau aaacuguucu uauacuacac acaaauggga     2460

cgaugcaugu acaguggaga aagaauagaa cucagagauc uauucaacaa aaacuuauau     2520

gacauugauc acauuuuccc ccgaaguaaa guuaaagacg acucaauuga uaacagggua     2580

uuaguaaaga aacaaauaaa cucacacaaa gauaacgacu auccucuaga uaguaccgua     2640

agggagaaaa ugagaggaca uuggcaauac uuacuacgac auggcuucau cagcaaaaag     2700

aaauaugaca gacuuacgag ggucacaccc cuuacagaug acgaacuagc agauuuuauu     2760

gcacgucaau uagucgaaac aucacaauca acgaaagccg uagcaacgcu auuaaaaaca     2820

cuguauccua caaccgaaau cgucuacguc aaagcggaac ugguuucuag auuucguaca     2880

gaauauaagu ucauuaaaug ucgagaagua aacgaccucc accaugcaaa agacgccuac     2940

uuaaauaucg uaguaggaaa cguauacaau gucagauaua cucauagcaa agccauauuc     3000

auaaaggguc uccaaaccaa gaaauauuca cuaaacagaa uguucgcauu ugacacaccu     3060

ggagcaugga auaucgaaaa ugaaaaaucc uugucaauag ucaagaaaac aguaaugaag     3120

aacaacaucc uauauacgag auauagcuau augaagaaag gaggauuauu uaaaauucaa     3180

ccccugaaga aaggaaacgg acaagcuccu cuaaagaaag accucccauu aagugacaua     3240

gguaaauacg gcggguauga caaacccaca gcuaguuauu ucuccuacau aaaauaugaa     3300

gguaagaaag gcaaggagug uagacaauug guggcuauag acaauuaucu uaagaaauac     3360

uacgaggaga acccaauaga auaucuaacg aauagacuag ggcucaaaaa ucccgcagua     3420

uuaauaccgg uaaucaaaua caauuccugu auagagauug acggauuucg gaugaauaua     3480

ucaucaaaaa guaacgaagg aaaaacaaua gucuacaaac cugccgucca gcuuguccua     3540

ggcgcagaaa aagaagcaua cguuaagaaa auauccaaag ugcuaucaaa accugaagaa     3600

cagaaaauaa caucauacga cggcgucagc gcagaggaaa acauugaguu auuugaugca     3660

cuaacagcaa aaaugaccaa cacuauauuu aaaaucaaac uagaagacau aggcaauaaa     3720

auaaaauccg gacgagauaa auuuguaaca cuagacguaa agaaacaaug cuacgucauc     3780

aaugaaauuu uaaaaauaau gcauugcaac guagucacag gagaucucag ucugauagga     3840

gcagcaaaga aagccggaau ugucucaacu aacucagcau uaucugagau caagaaagug     3900

ucaucaauua uguuaauaaa ccaaagugua accggacuau ucgaaacgaa aaucgauuua     3960

cuuaacauau ga                                                         3972


<210>  5255
<211>  3975
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5255
augcgcgcaa aagggaagac acuaauagga acgucucuau uccgagaagg aaacacugca       60

gccgaaagac ggggauacag gacuacucaa agaagacuaa aacgucgcaa aagacgucuc      120

aaacuauugg aagcauuuuu cgauccauau auauuugaag ucgaugagaa cuuccuagcg      180

agacuacacg aagcagacau aaguccaaag gacgaaaaca aacgauucug gggcagccua      240

uuauucccua acgaaaaaga ugccaacuuc uaccgcaagu auccaacaau auaccaccua      300

cgaaaagcuc uaauggaaga agauagaaaa uucgacguaa gagccaucuu ucucgcuauu      360

caccauauca ugaaauauag aggaaacuuu cucagccccc ugucagucga cucguuuaaa      420

caagaaggac uugaguuaaa agaacaacuc gaucgacaac uuaaacgcgu ugcugaauua      480

uauaaugagc uaaauacgga cguaaagauc aauguaagug acguagaaac uauccaagac      540

auaauaauug acaaaaauaa aucaagaaga gauaaagcag gagcacuaaa gaaacuccua      600

gccguaagua gugacaggaa agaaauaaac aaacuaaacg caucccuagc aaaagaauua      660

ucuaaugcca uccuaggcau gaaagcacaa uucgcaaauc uauugcuagc cgaugacuug      720

gaaauagaua aaaauaaaaa aaauuucagu cuccucgaca cuaacgccga agagaacgua      780

ucaggacuac uagaacuugu aaacgaaaaa caagcagaac uauuugacgc uauuaaaaca      840

auacacgacc aaaucauacu aaaugaaauc guaccagacg gaaaaucuau aucacaauca      900

augguagaca aauacgaggc acauagagaa caacuccagg uguuaaaagc acuauacgaa      960

aaaauggacg cgaaacaaau aaaagcaaua aaaaaggcau auucacuaua cauagauggu     1020

aaagacccaa aagcaaaaaa aguagauaaa agagcacauc aaaauagagc ugacuucuau     1080

aaagaaguua aaaaaguucu aguugagcua gacgacagca gagcagaaaa auuacucggu     1140

uuaauagaga ugggacaauu ucuccccaaa cagagaacag ccaugaacgg cguuauaccc     1200

aaccaauuac accaagugga acuagacaaa auaaucgaaa ugcagagcaa auacuaccca     1260

ugguuagcag aaaaaaaucc agucaaauca caccaaaaaa gagcuccaua caaacuagau     1320

gaacuccuua cauuucgagu ucccuauuau guaggaccua ugguaacauc caaaaaacaa     1380

cagcaaacaa gcaacuccaa uuucgcaugg augguuagaa aacaagaggg cgaaaucacu     1440

cccuggaacu ucgaagaaaa aguagauaca auugcuagcg caaaugacuu uaucaagcga     1500

augacagcga aagauaccua uuuagucgga gaagacgucc uccccgcuaa uagccuauua     1560

uaccaaagau acaagguacu uaaugaacua aauaacauca aagucaacgg aagaagauua     1620

ccagucgagu uaaaaagaga agcuuauguu gcuuuguucg aaaauggaga uucaaaaaca     1680

guaucagcaa aauguuuuaa aaacuggcuc aauguuaaac uaaaaaaccu uaauagucua     1740

aaguuagaag gauuaaguac uggaacgaaa uuugacaaua aucuaucaac auaccaugau     1800

cucaaaaaaa uauucgguaa agcaguagau gaugcgggac gacaaaaaga cuucgaacaa     1860

auuauagaau gguuaacaau auucgaagac aaaaaaaucc uaagaaucaa acuaaaagag     1920

auagaauggc uaaccgaaga gaauauaacu caauuaacaa gauuaaacua uaauggaugg     1980

ggacggcugu cuagaagacu uuuaacagga auuugugacu caaauggaca acgaauaaug     2040

gacuggcuau gggauacuac aaacaauuuu augcaaauac agucacaacc ugacuuccaa     2100

aaggcaauug uagaauauaa uuccgauaaa uuggaacaag gagauauaga acaaauacua     2160

ggcgaagcgu acacuagccc gcaaaacaaa aaagcuauaa gaaagguaau caaagucgua     2220

caagacguug aaaaagcuau gggagcaaaa ccaacaagca ucucaauaga auuuaccaga     2280

ucagauggaa gaagugaguu aacaaacagu cggaugacac aacuacagaa aacucuuaug     2340

ucacuaucag aagaaaugac ugaucaagca cuauuagcag aauucaaaga cuuccaagcg     2400

ucuaaaaaaa aacuaacaaa uaaauauuau cuauauuuua cucaacuagg gagagacaug     2460

uacacaggag aaccacuaaa uaucgaucaa cucucauuau acagugacgg agaccauauu     2520

cuaccacgca guuucaucaa ggacgauagu uuagauaauc gaguacuuac aaaacgggcg     2580

guaaauaacg aaaaaaguga uaccguucca guaugugaau acggcgcaaa aaugaaagac     2640

uuuuggaaug accuuuacga uaauggacua auaaguaagc aaaaacuagc aaaccuacug     2700

acggacccgg acgaaauagg accauacaaa gcaaacgguu uuauaagaag gcaauuaguc     2760

gaaacuucac aaguaauaaa acugagugca gaaauucuac aaacacuuua ucaagacacg     2820

aaaauuauug aagucagaca gaaauauacu caugaauuac gcgaacacuu uaacuuuguu     2880

aaaauacgac aacuaaauga cuaccaccau gccuucgaug cguaccuaac cgccuucagc     2940

ggacgauacc uauaccaaag guauccaaaa cugaggggau acuuugucua uggugacuac     3000

aacaaauuug augcaaaaaa agacagaaac uaccuuaaau cauauaacuu ccucuaugau     3060

cucgaagaag gaaaaacaga ucaaaucguu gacaauguag aacacaaagu guuuaugaac     3120

cggcaagacg caauaaauca auuaaaaaaa gcauacgaau auaaauacau guuaguaagu     3180

caagaaacuu auaccaacuc aggcgcaaug uuuaaggaaa ccaucuaccc cgucaauccu     3240

aaacaaaaac uaauucaaaa aaaaaacgac aaacccguag auaucuacgg agguuauagc     3300

aauaauuaug acgccuaucu uucaauugua agauuagaag acaaaaaagg aaucaaauuc     3360

agggucgucg guauccccau gcgaaauaaa gcagaguuag auaacuuagc aaaagaagac     3420

cauaauggau auaugcaacg gcuaaaugaa aucguagacg aacaaauaaa cagcucaggc     3480

aaaaaaaaaa cauucagaau uauacuacca aaaguacacu uuaaacaacu aguugaagac     3540

gacucuggca aauuuaugcu aggauccgca gccuauccuu auaaugccaa acagcuuguc     3600

uuaucaguua aaucaauaga aucccuagcu aacaaggaug caagugacag agaacuuguu     3660

gcgguauacg aggaaauuuu aaauguaguc aacgacaaau ucacccuaua ugacgcaaga     3720

caauuucgga acaagcuuac caguucaaaa gacaaauucg ucgaguuaaa cacugcugac     3780

aaaguaaccg aacuauuaga cauccuagau ggccuacaug caaauccagu uguuaaaaaa     3840

cucaaaauaa uaagcggaag ccccuucgga uucuugcaaa gaccuagcgg cauaguucua     3900

acagaaaacg ccaaacucau cuaccaaagc ccuacuggaa uauucgaacg aguaguauau     3960

cuuagggacc uauga                                                      3975


<210>  5256
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5256
auggaaggac aaaugaaaaa caauggaaau aacuuacaac aagggaauua cuaccuggga       60

cucgacgucg gcacaucauc agucggaugg gccguaacag acacagacua caacguccua      120

aaauuccgag gaaaaagcau guggggagca cgacucuucg acgaagcauc uacagccgaa      180

gaacgucgca cccaucgagg uaacagaaga agacuagcca gaagaaaaua ucgccuacua      240

uuacuagaac aacuauuuga aaaagagaua agaaagaucg acgauaacuu uuuuguaaga      300

uuacacgaau ccaauuuaug ggccgaugac aaaagcaaac cauccaaauu uuuacuauuc      360

aaugacacaa auuuuaccga caaagacuau uuaaaaaaau acccaacgau uuaccauuug      420

agauccgacu uaauacacaa uucaacagaa cacgauauaa ggcuaguauu ucucgcauua      480

caccaccuaa uaaaauaucg cggucacuuc auauaugaca auagcgcuaa uggggacgua      540

aaaaccuuag acgaagcagu aagugauuuu gaagaguacu uaaaugaaaa cgauauagaa      600

uucaacauug aaaacaaaaa agaguuuaua aacguccuau ccgacaaaca cuuaacaaaa      660

aaagaaaaaa aaauuagucu gaaaaaacuc uacggagaca uaacagacuc agaaaauauc      720

aauauuucug ucuuaauaga aaugcuaucc gggucaucaa uuaguuuauc caaccuuuuu      780

aaagauauag aauucgaugg aaaacagaau cuuagucucg acucagacau ugaagaaaca      840

cuaaaugaug uaguagauau acuaggcgac aauauagacu uauuaauuca cgcgaaagaa      900

gucuaugaca uugcuguacu aacuucgucg cucggaaaac auaaguaccu cugcgacgcc      960

aaaguagaac uauucgagaa aaauaaaaaa gaccugauga uucucaaaaa auauauuaag     1020

aaaaaccauc ccgaagacua caaaaaaaua uucaguucac caacagaaaa aaaaaacuac     1080

gccgcauacu cacaaacaaa caguaaaaau guuugcucac aagaagaauu cugccuauuu     1140

aucaaaccau acauccguga cauggucaaa ucagaaaaug aagacgaagu acgcauugca     1200

aaagaaguag aagacaaauc guuuuuaacg aaauugaaag gaacaaauaa uuccguaguc     1260

ccauaucaaa uacacgaaag agaacuaaau caaauccuca aaaacauagu cgccuacuua     1320

ccauucauga augacgaaca agaagacauu agcgucgucg acaaaauaaa acugauauuc     1380

aaauucaaaa uacccuacua cguaggaccc cucaauacaa aaagcaccag gucuuggguu     1440

uaccgcucag augaaaaaau uuacccaugg aacuucagua acgucauaga cuuagacaag     1500

acggcucacg aauuuaugaa ccguuuaaua gguagaugua cuuacacuaa ugauccuguu     1560

uuaccaaugg auagucuacu auauucaaag uauaacguac uaaacgaaau caacccaaua     1620

aaagugaacg gaaaagcaau accaguagaa guaaaacaag cuauauauac agaccuauuc     1680

gaaaauagca aaaaaaaagu aacucgcaaa agcauuuaca uauaccuacu gaagaacgga     1740

uacaucgaaa aagaagauau cgugucaggc aucgacauag aaauaaaauc uaaacucaaa     1800

agucaccaug auuucaccca aauaguacaa gaaaacaaau gcacaccuga agagauagaa     1860

agaaucauua aaggcauauu agucuauucc gacgauaaaa gcaugcuacg aagauggcua     1920

aaaaacaaua ucaaaggccu uucugaaaac gauguuaaau accuagcaaa auuaaauuau     1980

aaagaauggg gacgccuauc aaaaacccuc cucacugaua ucuauaccau caauccagaa     2040

gauggugaag caugcucaau uuuggacaua auguggaaca ccaaugccac acucauggag     2100

auacuaagca augaaaaaua ucaauucaaa caaaacauag aaaacuacaa agcagaaaac     2160

uacgacgaaa agcaaaaccu acacgaagaa cuagacgaca uguacauauc accggcagcu     2220

cgcaggucaa uauggcaagc acuucgaauc guagacgaaa uuguagauau aaagaaaagu     2280

gcuccuaaaa agauauucau cgagauggcu agagaaaaaa aaagugcaau gaaaaaaaaa     2340

cgaacugagu cucguaaaga cacacucuug gaauuauaca aaagcugcaa aagccaagcu     2400

gacggauucu augaugagga acuauucgag aaacuaagca acgaauccaa uucacgccua     2460

cggagggauc aacuauaucu uuauuacacc caaaugggaa gaagcaugua uacaggaaag     2520

cgcauagacu ucgacaaauu gauaaacgau aaaaauacau acgauauuga ccacaucuau     2580

ccacgaucaa agauaaaaga ugauucgaua acaaacagag uucucguaga aaaggacauu     2640

aacggcgaga aaacugauau auauccgaua uccgaagaca uccgacaaaa aaugcaaccu     2700

uucuggaaaa uauuaaaaga aaaaggauua aucaacgaag aaaaauauaa acgacuaacc     2760

cgaaacuaug aauuaaccga ugaagaauua ucaucauucg uagcaagaca acucgucgaa     2820

acacaacaga guacaaaggc ccuugcaaca cuauuaaaaa aagaauaucc aucagccaaa     2880

auaguauauu caaaagcugg uaacguaagu gaauuucgca accguaaaga caaggaacua     2940

ccaaaauuuc gggaaauaaa ugaccuacau cacgcgaagg augcuuaucu gaacauugua     3000

guaggaaacg uguaugacac aaaauucaca gaaaaauuuu uuaacaacau uagaaacgaa     3060

aauuacuccc ugaaaagagu auuugacuuc ucaguacccg gggcuuggga ugcuaaagga     3120

agcacauuca auaccauaaa aaaguauaug gcaaaaaaca auccgaucau agcuuucgca     3180

cccuaugaag uaaaaggaga acucuuugac caacagauug ugccaaaagg uaaaggucag     3240

uucccaauaa aacaaggaaa agauaucgaa aaauacggcg gcuauaauaa auuauccucu     3300

gcguucuuau uugcgguaga auacaaagga aaaaaagcca gggagcguuc uuuagaaaca     3360

guauacauaa aagacguuga auuguaccua caagauccaa uaaaauacug cgagaguguu     3420

uuaggucuca aagaacccca gauuaucaag ccaaaaaucc ugaugggaag uuuauucuca     3480

auaaauaaca aaaaacuggu agucacagga cgaucaggaa agcaauacgu cugccaucau     3540

auauaccaac uaagcauaaa cgacgaagau ucucaauacc uuaaaaacau agccaaauac     3600

uuacaagaag aacccgaugg aaacauagaa cgacaaaaca uauuaaacau uacuucagua     3660

aacaacauca aacucuucga uguacuuugc acgaaauuua acagcaauac auaugaaaua     3720

aucuuaaacu cgcuaaagaa cgauguaaau gagggaagag aaaaauuuag ugaacuagac     3780

auacuagagc aauguaacau uuuacuacag cuccuaaaag cauuuaaaug uaauagagaa     3840

agcuccaacc uagaaaaacu aaacaacaaa aaacaagccg gaguaauugu aauuccccau     3900

cuauuuacga aauguucugu auucaaagua auacaccaau cgauaacagg auuauucgaa     3960

aaagaaaugg auuuauuaaa auga                                            3984


<210>  5257
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5257
augaaaaauu acucaauagg ccuagacauu gguacgggau caguagggug ggucuguauu       60

gaugaaaauu aucaaauauu aagauauaac aacagagcug cuuuuggagu acaugaauuu      120

gaaacggcac acguagcaga agaacgaagg auaaaaagag gagcaaggcg aagguacaau      180

agaagaaaga aacgaauaca acuacuucaa ucccuauucg uaccacacau gcaacaaaaa      240

gccuucuucg cuccgacgau aacugaacau uacuggaaaa augauaacca auuugaaaac      300

agaacccucu cagaaguacu aaugagccua ggcaugaacc cuaacacuua ucccaccaua      360

uuccaccuaa gacacgaacu uaucgaauca aguguaaaga aagacgcucg ucucauauau      420

cuagcgcuuc auaaucuugu gaaauuucga ggccacuucu uacaagaggg uuccacuugg      480

aaugccaaua ugucagacgc caacuuaucc gaacaauuug aacaacuccu acauauauau      540

accgagaugg aagaauuacc ccaaguauca uugaccgcac aacaauccac cgauguagaa      600

cauauucuuc uaaacaacca uauaagcaaa gcugaaagag cacgccaauu gacagcauua      660

uucgagcgua cauaugaacc uuucuuuaaa cuaauaguag gauuagacau cgcugcagcg      720

caacucuuca aacaaucaga acaacaacuu uuauauaaag aaaguaaaac aaaaguaaac      780

cuuacagcug aaacccuacc cgaagugcua caaaccaugc uggaaccaga ggucgcuuuc      840

auagaagcag uucaaccauu uuaccaacaa accguauuau ucgaccuacu aaaaggucaa      900

agcuacgucg cagcugcaaa aguggaagca uucaaccaau acgaaagaga ccuagcucua      960

cuuaaaagug uaaucaacga agucggauca gaaaaacaau acagagcauu cuuuauuaca     1020

ucaaaacaaa agaaacgaca auuugagcaa aaaccaaaca uggaauuacu augucaucuu     1080

gauagauuua aucgcgagaa aaaguacgaa gaaacauucu auaaagaggu aucgaaacua     1140

cugacagcac uuaucaaaga agcaacggga gaaguaaaag aacacauuga acaaauuauc     1200

caagcaaugg aaucacaauc uuuccuagug aaacaaaaag guauucaaaa ugccuccauc     1260

ccauaucaaa auagccuaua cgaagcagaa augaucuuaa gaaaccaaca agccuacuac     1320

ccuuggauaa ccgaugaguu cauagaaaag guuaaacaac uuaucgcguu cagaauacca     1380

uacuacauag guccccuagu ucagggcgau gaacaaccau ucggauggcu ucagagacag     1440

gcugauggaa acauaacacc auggaauuuc gaugaaaagg uaaauaaggc agcaucagca     1500

gaaucauuca ucacacguau gaccaaaaau ugcacauauc uaaaagaaga aaaaguucuu     1560

ccaaaacaau cacuuaugua ugaaaaauuc acauuauuaa acgaacugaa cuccguacaa     1620

auacgaucau cacuagaaca accccacaag aaacaccgau uaugugaaga uaugaaacag     1680

uggaucauug aacacguauu caagaaaaca aaaacuguaa cccacaaaac acuaauacaa     1740

gccuuaaaga aaucaccaua caaagacgaa cuuuaugaug aaaguacagg uaacuggaaa     1800

gauguuuaug gaacgcaaaa agaagauaga uuugcaucuu cucuauccac auauauuuca     1860

uuaaaagaaa uaguaggcga uauugucgac aacgccuuua uggaagaaau aauauacugg     1920

cuaacaguau ucaccgauag agacaucaua accuuaaaaa ugaacgauaa auacccuuuu     1980

aucacugaac aacaaauaca aaggcucuua ucacuuaaua uaucaggaug gggacaauua     2040

ucacaacgac uacuucaaag caucccugua gaugacauga accgcucagu aauagaauau     2100

auggagaaac acgcaguaaa cuuuauggaa cucuuaucac ccagacaguc agauuuagca     2160

uacaaaaucg aacaaguuaa caaacaacac aguaaaaaga uacacaaaau aagauacgaa     2220

gacauagaac agcuuaccgg uucaccagcu cuaaaaagag guaucuggag aagcguuaaa     2280

aucaucgaag aacucacugc gauauuuggc gaacccaaaa auauaaugau agagguagca     2340

cgagaagauc aagcuagcca cagaacaaag uccagaaaag aacaauggga aucuauaggu     2400

aaaucacucg cuaaggaaga aaaagaauua aaagcauucu uuaaagaaau ggcacaauac     2460

cccaaacaac aauuugauag aacgagauuu ugguuauaug ucacacaaca agggaagugc     2520

cuauauacug gcaaaacacu cagacuggaa gaccuaucau ccuaugaaau cgaccacauc     2580

uuaccaagaa acuuuguaaa ggacgacuca auagacaacc ucgcccuagu cuauccuaug     2640

gcaaaccaag agaaaaauaa ggcagguaau aacaaaaugc ccuuagaaau cauagaugca     2700

aaacaacaac cagcaaugau ggcccacugg aaaagacuca aucaauuaaa acuaauaucg     2760

gacuccaaac uacauaaacu uaugaaaccu ucuuuuaacg auguagacaa agaaaauuuu     2820

auagccagac agcuaguaga aacuagacaa auaaucaaac acguucgcga ccuguuacaa     2880

gaacgauuug aacacacaga cauacacguu guuaaagcaa aaaugguauc acaauuucgc     2940

aaauucucca gaauaccaaa gauccgggac uauaauaaca aacaucaugc aauggacgca     3000

uuauucacga caguacuaau ccaauuuauu cuacaaaaau augguaaaaa uuuccuucaa     3060

uucgauuugc aacauaaaga uauaucaaag aaauggucaa aaauugccca aagcacaaaa     3120

cacuucuuuc uuuuucaaca auuugcugaa caacaaauaa aaucuccagu aacuaaagaa     3180

uuagucagug gaguucaaua uuuugaacac auauuuuaug aacaauccug gcaaacaacu     3240

aaaaugacuc aaacgauaga cucgaacuuc uacaaagaaa caauauuuuc acccaaacuc     3300

aaaacagcua aauauacaag ugcgaaagcc gaaaaauuug uucacgaugc aaucguaaag     3360

uacgcagucu guggaauaca auacacaucu uuaaagaaaa auaaagaaca acaucacgua     3420

uuguuaacug acaucaaugu cauagaacau uaccaauuaa aagaggcuuc cuugaaugaa     3480

uuagcucuac auuuagccaa aagagaauca aaagacgacg uaauagacgc gcaaaugcua     3540

gucguaauac caaaauauaa gaaaauuaug uuaaauggaa gcccguacua cugcauauca     3600

agugacgaac gccauaucgc cacucaauuc guacugagga aagacuuacu agaggcuguu     3660

cuccauguca augaugaaac aucuguugac gaacuaagaa cacucuucag gucaauaguc     3720

gaagagauga ugacacaaua uaagguauac aauaaagaca caaucagaaa uaaaauagau     3780

gcguuuauag aaacagaauu aauagacguu gaaacuuuuc aagucggauu uacugagaua     3840

aagaaaaugg uagcagcaaa cgcacaaaga uccgacaaau ucggcggaag acuaagacaa     3900

gucaugaaag ucgaagacau uuacauuaua gaugaaagca uaaccggacu cagauauagg     3960

aaaccaagaa aauuaguuaa auga                                            3984


<210>  5258
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5258
augaagaaaa acuacacuau uggauuagau auugggacug cgucuguagg uugggcaguc       60

cuaacagagg acuacgaauu aauaaaaagg aaaaugaaaa ucucuggcaa cacacaaaaa      120

aaagcgguua aaaaaaacuu uuggggugua agauuauucg aacaaggaga aaccgcagag      180

ggacgaagau ugaaacgaac aacaagaaga cgaauagcaa gaagacgaca aaggauccag      240

uaucuaagga cgauauucga cgaaggaaug aaccaaguag acgcaaacuu cuuugcuaga      300

uuagaugaga gcuuuucuau aauagaugaa aaagaaaacg aaagacaucc aauauuuggc      360

aauguagccg aagaagcagc uuaccacgaa caauucccca caaucuacca ccuaagagaa      420

cauuuagcca auucaucaga acaagccgau cuaagacucg uauaucuggc cuuagcacau      480

auuauaaaau ucagaggaca cuuccuaauc gaaggagaau uaaauacaga aaacucgagc      540

guaaguggca cauuugaaca auuuauaaaa guauauaacg aaacguuuaa ugucgaaaaa      600

gcacuagacu uaacaguaga ccuagacgga auagcagaac aaaaaauaag uagaaugaaa      660

agagcagaac uuauccucag ccuauucccu gaagaaaaau caaccgguga uuuugcucaa      720

uucauaaaaa ugauuguagg aaaccaagga aaugugaaaa aaaccuucag ucuuaaugaa      780

gacgcaaaaa uacaauucuc uaaagaagaa uacgaggaaa aucuagagac ccugcuagca      840

gaaaucggag aagauuuccg aucaguguuu gacgcggcaa aaucaguaua ugaugcaaua      900

agucuagcca acauacucaa aguaaccgau gcaacaacua gagcaaaauu aaguucaagu      960

auggucguga gauucacaga ucacaaggaa gaucuaaaaa cauuaaaacg auucgugaga     1020

gaaaaucuac cagaugaaua ugacgaucug uucaaaaaca aaaagguugc aggcuaugca     1080

ggauacauag acgggaauga aacacaagaa gcuuucuaca aauaccuaaa aaaaacacua     1140

gcgaaagcca caggcgcaga agguuuccuc gcuaaaaugg aacaggaaga cuuucuaaga     1200

aaacaacgaa cuuuugacaa cggaguaauc ccauaccagc uccaccuaga agaacuaaag     1260

gccauuauaa aaaaccaaaa aucguauuau aguuuucugg augaagaaaa aauaucacaa     1320

uuaaugacau uccguauacc uuacuauguu ggaccuuuag cuaaagaaaa aggucaauuc     1380

gccugguuaa caagaaagga aaugggaaaa auuacgcccu ggaacuuaaa ugaaaaaguc     1440

gacauagaaa aaucggcaac agacuucguc gaacgaauga caaacaauga uucuuaccua     1500

ccuauggaaa aagucuuacc aaaacacuca uuauuauacg agaccuuuac ugucuacaac     1560

gaacuaacua aaguacguua uauagaugac aacgggagag cccaaaauuu uagcagcaaa     1620

gaaaaaagac aaaucauaaa cgaacuauuu aagcaacaaa gaaaaguaaa aaaagaaaug     1680

uuggaagcau ucuuaaaaaa ugaauauggg auagaaaacc caaaagugga agggauugaa     1740

aaaagcuuua acgccuguuu aggaacauau caugacuuga uaaaguuggg aauuagaccc     1800

gaacuauucg aacaaccuga acacgaacaa caauuugaac aaauaguaaa aauccuaaca     1860

guauuugaag aucggaaaau gagaagaaag caacuagaaa aauucaguaa cauuuugaca     1920

gaagaagaac aaaaacaguu agaacgaaaa cacuauaaag gauggggcag auuaagugca     1980

aaguugauac acggaauagu ugaccaaaaa acacaaaaaa cgauacuaga cuaucuaaua     2040

gaugacgaug accuacccaa gaacagaaac agaaauuuca ugcaauuaau aaaugacgaa     2100

aaccucuccu uuaaagaaga aaucgaaaaa auugccuuug acaaugacaa gagcacagaa     2160

gaaauugugc aagaacuugc ggguucacca gccauaaaaa aagguauauu acaaagucua     2220

aaaauaguug aagaaaucau agauauaaug ggagaauuac caacuaacau agucguugaa     2280

auggccagag aaaaucaaac cacagcucaa gguaacagag ccuccaaagc aagaaugaag     2340

uaccucgaag aaucaaucaa aaagcuugga aguuccauac ucgaagauga accaauaucg     2400

aaagaugcua accuacuaag aaaugauaga cuguuucuuu auuauuuaca gaacgguaga     2460

gauauguaua caggaaauga acuggauaua aauaaccuua guucauauga uauagaucac     2520

auaguuccac aaucauucgu aaaagaugau aguauugaua acagaguacu aacaacuagu     2580

aguaugaaca gaggaaaauc aaacacgguc ccagcggagu cagucguaaa aaaaaugagg     2640

ccuacauggg aacguuuacu cgcuagugga cuaauaucga aaaaaaaauu caguuauuua     2700

acuaaagcua caaauggagg ucuuaccgaa gaagauaaag cacauuucau acaacgacaa     2760

cucguggaaa cacgacaaau cacgaaaaac guugcacaaa uucuccauca aaaauauaac     2820

aacgaacaac uaagcgaaaa accaguuaga guuauuacgc uaaaaucagc ccuggcuucc     2880

caauuccgaa aagacuucuc uuuauacaaa auacgcgaac uuaacgauua ccaucaugca     2940

caggaugcau aucuaaacgg aguaaucgca caagcacuau uaaaaguaua uccgaaacuc     3000

gaaccagagu ucgucuacgg ggaauaucaa aaaguuucaa uuagagcacu uaacaaggca     3060

acugcaaaaa aagaauucua uuccaacauc augaacuuuu ucacuucuga ugaaauggua     3120

gccaaugagg aaacuggaga gguauuaugg aauagacaaa gagacauaaa gacuaucaaa     3180

aaggucauga auuaucacca aaugaacauu guuaaaaaag uugaaauuca aacuggugcu     3240

uuuaccaaag aauccauauu accgaaaggu ccaucaaaga aacuuauugc aagaaaaaac     3300

aauugggcuc cgguaaauua cggagguguc gauagcccua caguugccua cucaguuauc     3360

auuacccacg agaaaggaaa agcagcaaaa guuguucaac aacuagucgg uauuaaaauu     3420

cuggaacguc aagcauucga acaagacgaa guagcguuuu uagaagaaaa aggauuuauu     3480

cacccgaagg uccaauuaaa auugcccaaa uauuccuuau aucaauuugc ugacggcaga     3540

cgcagacucu uagcaucagc cgaggaaagc caaaaaggca accaaauggu ucucccugua     3600

caucucaucg aauuacuaua ccacgccaaa cacgucucag auagcucugg aaagucacua     3660

gaauaccuca acgaacauag gcaugaauuc gcagaacuac uagaagccau auuacaguuu     3720

acagaacaau acauugacgc agggaaaaac caaaaaaaag uucgagaccu uuacgaaaaa     3780

aaccaagaug ccgacaugag agaauuagca uccucguuca uacaauuauu acaauuaaac     3840

aagcaaggag caccagcaga uuuuaaauuu uuuggagaaa caauaccuag aagacgauau     3900

aaaaauacag cugaaauagu cgaugccaca uucauaaacc aaagcaucac agguuuauau     3960

gaaacacaaa aaagguuagu auga                                            3984


<210>  5259
<211>  3984
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5259
augaccgaac aacaacaacc auauaacauu gggcuagaca uaggcacauc aaguauugga       60

ugggccguaa cugaucaaaa ugacaaguua uuacacauca ggggacacaa cggaauaggc      120

guuagacuau uuaaagaagg agaaucagca gcagaaagac gaggguuuag aacaacgagg      180

cgcagacucu ccagaagaag auggcgucua agauuucucg auaggauauu ugacgcugaa      240

uuagcaaagg uugaccccaa uuuccuagca cgucucaaac aaucaaauau uuccccacua      300

gaccccaaua aacaaauguu cgggaauaua cuauucgaug acccacaauu cgaugaccaa      360

aaguuccauc aacaauaccc uacuauauac cacuuaagau cagcacuggc acaaccagau      420

gcaccccaag cagacgucag auuaauauac cucgcgcuac accauaugau uaaauucaga      480

gggcacuuuc uaaacggugc ugcaguagaa aaauuucaug gcggacaacu ccauuuagag      540

ucagaguucc aagaacuuaa ccaaaucuuc ucaaaccagc agcgagacca uguucaacua      600

caaacuucaa gucuagauga aguagcgaca auacuaaaag acaaucaacg cacaccgagu      660

gauaggcaac gcgaacuuag ugcuagauua uuuguucccg uggauaaaac aauagacaag      720

gaaaacaaga aacucgccac agaauuacua aaagccgugc ucggauuaaa agccaaauuu      780

gacguaauac uaggggugga agccaccgac ccacaagcau ucaaacuaca guucucuuca      840

gacgauuucg augacaaaau agcagaacua gcuagcgaac uaaccgacga agcaaacgaa      900

auccuaacag uacuccaaag guuauacuuu aguuuaaauu uagcggauau acuaaccaac      960

agacaaacaa aacaagucgc ugacucgaua ucaacagcaa ugauaaaccg uuaugacgac     1020

cacaaacacc acuuaaaaau guugaaagaa cuggaagcgc aaguaacgca agaacaacgc     1080

gauggacuaa agaaagccua uaacgaauac auagacgggg acaaacccac aauugacacg     1140

uucuacacca acguaaagaa aaaucuagau gauagugaau uagcaaauca aauuaaaucc     1200

uugaucgauc uucaagccuu caugccaaaa caaagaucaa aagaaaaugg aucaauacca     1260

caucaacugc accaacgaga acucgaucaa uuaauagagg uccaaggaaa auacuauccu     1320

uggcuccuag ccgcaaaaga caaacuagau gccuuaauag ccuuucgugu acccuacuac     1380

guaggcccaa ugauagaccc uaaucaauca gaugacccac agaacacuaa auuugccugg     1440

augguaagaa aagaagcagg agaaaucacg cccuggaacu uugaagaaaa gguagacaaa     1500

acaagcuccg ccacuaacuu cauaaaaaga augaccacua cagacacgua uuuauuagga     1560

gaaccgguau uaccuaaaaa cagcuuauua uaccaacaau uuacugucuu aaacgagcug     1620

aacaaacuaa aagucaacgg aaaagcacua acuguagacc aaaagaaaua cuuauacgau     1680

cauguuugua agaaacaaag acgaguuacc cuaaagaaau ugucagauug ccuuguagcu     1740

aggggugauu ucccuauggg cgcaauauuc acaggaacaa cagaccagaa aaauaugaau     1800

aauacacuca aaaccuacca agacuuccaa gaaaucuucg gcgacgaaau agauaaucca     1860

gauagacaag uugauuuuga gaaaaugaua gaauggagua caaucuuuga agaucgggaa     1920

aucuucaaac uaaaauuagc ugaacucaac uggcucacaa gugaacaauu agaaagggua     1980

ucaaagaaaa gauaucaagg auggggucgc cucagucaaa aacuguuaac ccacaucgua     2040

aacgaaaaug gagagcgaau aaucgaccaa cucuggaaua cuaaucaaaa cuucauggaa     2100

auaaugggac aagauucugu caaacaagca auacaagaac auaauggcgc aaacguugcc     2160

caaacuggaa uaaacgaaac aauugaugaa uuauacacau cuccacaaaa caagaaagcc     2220

auaagacaaa uacuccuagu aguugaugac aucaagaaag caguaggaua ugcaccagcc     2280

aauauaauga uggaauuugc aagagaagau agggaugacc acagacuaau agcuaguaga     2340

gcacgucaac uagagaaaac uuaucaagaa auaaguaaaa auugguuuga caaugagucc     2400

guaacucaag aacuaaaaga ucaaauuaaa aacaaaacga aauucacaga ccgacuauau     2460

cuauacuuca uacagggagg aauagaucuu uauacagguc aucccuuaaa cauagaccaa     2520

cuaaguucau augacauaga ccauauuuua ccgcagagcu ucuuacuaga uaauucucuu     2580

aacaauaggg uacuaaccuc ccaagcagaa aacagaguac auaaagaaga uagacugccc     2640

ggagagaccg ucgcaaauaa aaugaggccc acuuggaaaa ugaugagaga acaaggacuu     2700

augacacaaa gaaaauacaa uaaucuaacu cuaaaaccag accaaauauc caaauuuaau     2760

aauaggggac aauucaucaa ccgucaacuu guagaaacaa gacaagugau aaaauuggca     2820

gccgacauaa ugucacaaaa auaccaagca gaucaaacua aagucguaac aguaaaagca     2880

aacuuaacac accacaugag agaaaaauuu aauuucuaua aaucuaggaa cguaaaugac     2940

uaucaucacg cuuuugaugc cuaucuaaca cuauucgucg gaaacugguu auuaaaagaa     3000

uauccgaaac uacaaccaua uuucguauau ggugacuucg ccaaaacagg aacgaacaac     3060

uuaaaaucuu ucaauuuccu auauagauuu gaacgagacc acaaauuaaa cgacaaaggc     3120

gcuauugaca cugauaaaga ccaacaauua ggauauauga aaaggauuua uagauucaag     3180

aaaaugcuag ugaccaaaca acuugaaaca aacgaaggag cguuauacgg ucaaacacua     3240

uaucccgcuc cuaaaaauga caagaaaacu cgaaauuuaa uaaauucaaa gaaaaaucgc     3300

ccaagcgaca uauauggcgg uuauucuaac gaaaacggga gcuuccuaac auuagugaaa     3360

cucaagaaag gcucaaaaua cgacuauaga uugguagcug uaccagucuc aaaaauaagu     3420

gaaauuaaag aaucugaacu agcuacuaua gaaaaagcaa uagcaccaca auuccuaaaa     3480

ucaucaggaa aauaccgaga cuuccaaguu guacuaucaa aaauccuaau aaaccaaaaa     3540

guaaucgacg augguaugau auucacaaua ggaucuaaau cauacaaaca uaacgcaaaa     3600

caaguagucu uauccgauaa cagcauacaa auagugagca cccuuucaag aaucucagac     3660

aaagaaaaac uaaucacagu auuugaugaa auaguagaca uaacgaguaa auacuuacca     3720

ugguacaaag uaaacgacgu agagaaaacg aaaaugcuau ucaggcaaau gcaagauauu     3780

gacgaaauga aaaagauuau agacaauagu cuuauaggcu uacacugcaa cgcaagcaua     3840

acagauuuag gaaaaguagg cuuaaguaag uacuggggaa gauucguauu aacagcaggu     3900

auaaguuuaa caguagacgc ccaauuaaua uaucagucac caaccggacu guuugaacau     3960

agagucaagg uaucggauuu auga                                            3984


<210>  5260
<211>  3990
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5260
augaacaaac aaccuuauaa caucggacug gacauuggca ccucauccgu gggauggagu       60

gccacagaac uagacaacag gcuacuucau auaaaaggac auaacgguau uggaguacgu      120

cuuuucaaag aaggcgaauc ggcggcugac aggagaggau ucagaacaac ccgacgaagg      180

cuugcaagac gaaaauggcg acucagacuc cuaaaugaaa uauuugccac ugaaaucgcc      240

aaaguugauc cuucauucuu cgcaagacuc aaacaaagca acguaagucc aaaagacccc      300

aauaaaacaa uguucggaaa cauacuuuuu gacgaugaaa accuggauga ucaaaaauuu      360

caucaugacu auaagacuau cuaucaccuc agacaagcaa uaauaaacca uccugagcaa      420

aaauucgaca uaagguuaau auaucuagca augcaccaca uaauaaaaua ucgaggucau      480

uuucuaaacc aagcaaaugu acaagauuuc aagggaggca aaauagauuu agaaaaaucu      540

uuuaaagccu uaaaugacau auucgaaaau caagggagag aucuuagauu aguaacugac      600

gaggcaaauc aauacgucaa aguacucucc gauaacgcaa aaacucgauc agaccguaaa      660

aaagaacuau cuaagcuucu guauguacca auagauaaag acacagacaa gaacucuaaa      720

aaagucacag gagaaauacu aaaagcuauu cuuggaaaua aagcaaaauu cgauguuauc      780

uucggcauag aaguagaaaa ccccaaagaa uggguauuaa cuuucaacag ugacgacuuu      840

gaugauaaaa uagaaggacu cuccucacaa augacagacg aagauucaga aauccuauua      900

auacuaaaag aauuguauuu cgcuuuaaau cuaagugaca uucuaaauaa cucuguuacc      960

gauaagaaug caaaaacuuu aucagaagcu augauaaaua gauacgauga ccauaaaaac     1020

caacuagcga ugcuaaaaaa aguaauagac ucaucagaca aaaaacacgc aaaagagcua     1080

aaagaagcau auucagccua uaucgauggc gaaaauaaca aaaaaaucag cuucgaagac     1140

cucaaaaaaa gaauacaaaa aaaucuaguc gauucagagu uaagucaaaa aaucaaugac     1200

cuacucgacc augauuauuu ccucccaaaa caaagaucua aggaaaaugg agcuaucccu     1260

caucaauuac aacaacaaga auuagaccaa auuauagaau cucaaaaaca auauuauccc     1320

uuucuagccg aacuaaaccc aaaugaaaaa cguagcagac aagcaaaaua caaacuagac     1380

gaauuaguag cuuuucgagu uucuuauuac gucggcccaa ugguagaagu aacugacagc     1440

aaccaagaaa acaauggcaa auucgcaugg augauuagaa aagaacaagg agaaauaacu     1500

ccauggaauu uugacgagaa agucgaucga acagaaacag cuacaaaauu cauuacacgc     1560

augacuacaa ccgacacaua ucuaaucggu gaaccaguac ucccgaaaaa uagucuccuc     1620

uaccagaaau uuguaguacu gaaugaacua aacaauuuga aaauaaacca agaaaccaua     1680

acaccuggac aaaaacaaua cuuauaugaa caugucauga aaaaaaacaa gcgcguaacu     1740

uuaaagaaaa uagcagacgc auuagugaua aaaggugauu accccuacgc acccgaaguu     1800

aaagguacga cggaccagaa aaauuuaaau aacggccuca ccaccuacau ugacuuagcc     1860

aaaaucuucg gaaacgaacu agaugacguc gaccgcgaag ccgacuuuga aaaaaucaua     1920

gaauggucua caauauucga agaccaacac auauucgucu uaaaacuaca agaacuaaaa     1980

uggcuaacag aaaaacaaag aaaucaaguu guuaaacucc guuaccaagg auggggaaaa     2040

cuaucgaaag aacuacuugc cggacuugua gacgacaacg gacagaggau caucgaccuu     2100

cuauggucug acaaccaaaa cuuuaugcaa aucguaaacc aaaaauccuu uaaagaaucu     2160

auagcugauc auaauggagc acaucucgaa gagaaaaacc ugaaagacgu aauaaacgau     2220

cucuauaccu ccccacaaaa uaaaaaagcc auacgccaag uuauacuagu agucgacgau     2280

auuaucaacg caguaggaua cgaaccagcu aacaucauga uggaauucgc acgagaagau     2340

ucaaaugacc acaggcuaac aaacucacgu aguagacaac uugaaaaugu cuacaaagau     2400

auaacuaauu ccugguuuga uaacgacucc guaaaagaug agcuagaaga caaaauaaaa     2460

aauaaagaca aauuuacaga ccgauuauac uuauauuuua cacaaggagg caaagauuua     2520

uauacuggcg aaccgcuaaa cauagacgac uuaucgucau acgacauaga ucauauauua     2580

ccgcaauccu uuauacuaga caauucauua gacaaccgcg uccuuacuuc acagcgaaau     2640

aauagaguaa guaaagaaga ucagcuuccg ggagaauuau ucgggacuaa aaugcacgcc     2700

uucuggaaaa gauugaaagc agcucaccuc auuucaaaua aaaaauacua uaaucuaaca     2760

uuaacaccaa auagcauauc uaaauuuaac aaucaaggca gauuuauaaa ucggcagcua     2820

guagaaacac gacaaguaau aaagcuagca gcagaaauac uccacaaccg uuacagcgaa     2880

gaaaaaggaa cuaauaucgu aacgauuaaa gcaaacuuaa cacaccacau gcgagaaaaa     2940

uucaacuuuu acaaaaacag aaauguaaac gauuaccacc acgcauuuga cgcauaccuc     3000

acagcuuuug ucgguaauug gcuuuuacaa caauacccaa aauuaaaacc auacuuuguu     3060

uauggagacu ucgcgaaaac agaaauaaau aauuuaaaaa guuuuaauau gcucuauaaa     3120

uucgaacaag cacaccaacu uaacgaaaaa gacgguaaaa ucgccaaaga uaccgacgca     3180

cuacuaggcu auaugaaaaa uguauaucau uuuaaaaaaa ugcuuguaac caaagaacug     3240

gaaacuaauc acggcgcccu auuuaaccaa acacucuacc cuucaccagc acaugacaua     3300

aaaaaacgac aguuaauaaa accaaaggac aaucggccua ccgauauaua cggaggauau     3360

acccaugaag aaagaaaaua caugucccua gucaaaguaa uugggaagaa agauaacgaa     3420

uaccguaugg cgaacguccc acuccugaaa aucaacaaga acaacgauau uaaagaaauc     3480

cuaaaaucac aauuuucaaa caagaaguuu gaaaucguac uuccuaaagu acuaaaaaau     3540

caacuauucu cagaaaacgg ugcccuguuc gugaucggau ccaaaggaua uaaauacaau     3600

gcaaaccaac uaguccucuc ugaccuugac augaaacucc ucaaugaccu gucuaauaac     3660

aagaguauag augaaguaac aauuaaccaa cucuucaaaa auaucguaga uagcacuaac     3720

aaauauaugc ccuuauacga aauaaaaaac uuaaacgaag ucguagaaaa auuuaacaac     3780

cuagaauuua augauaaaaa aaaaacuaua acugacuucc uacuugcuac ccacgcaaac     3840

ccaacgauga gcaacuuaaa aaauauagga cuaccuuacu ucggacgauu caucagaaac     3900

acagguauca aguuauccga aaaugccaac guaaucuacc aauccccaac aggacucuuc     3960

uccaggaaaa ucaaaauauc cgacuucuga                                      3990


<210>  5261
<211>  3993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5261
augaaaaacg acuauaccau aggacuagau aucgguacga acucaguagg auauucugua       60

guaacagacg auuauaaagu aauauccaaa aaaaugaacg uuuucggaaa uaccgagaaa      120

aaaagcauca agaagaacuu uuggggagug cgccuuuuug aaucaggaca aacagcccaa      180

gaagcgcgca ugaaaagaac gucacggcga cgaauagcac gucgaaaaaa cagaauuugu      240

uaccuacaag aaaucuucca gccagaaaug aaucacuuag auaacaacuu cuuuuauaga      300

uuaaacgaau ccuuccuagu agccgacgac gcgaaauaug acaaacaucc uauuuuuggu      360

acucuggaug aggagaucca uuuccaugaa caauucccaa caaucuauca cuuaaggaaa      420

uacuuggcag acggagacga aaaagcagau cuacgucuag ucuacuuagc aauagcacau      480

aucauaaaau uccgcggaaa uuucuuaaua gaaggagaac uaaauacgga aaacaauagc      540

gucauagaac uauccaaagu auuugugcaa uuauauaauc aaacgcucag cgagcuagag      600

gguuuucaau uuauagauga aucgauugac uuuagcgaag ugcuaacuca acaacucuca      660

aaaagcgaac gagcagacaa uguacucaaa cuauuuccag acgaaaaagg aacaggaauu      720

uuugcgcaau ucauaaaauu aauagucggu aaccaaggaa acuuuaaaaa aguauuccaa      780

cuugaggaag accaaaaauu acaacuaucu acagacgacu acgaggaaaa uauugaaaac      840

uuguuagcca uaauaggaga cgaguacgga gauauauuug uagccgcaca aaaucuauau      900

caagcaauau uacucgcagg caucuuaaca ucaaccgaaa aaaccagggc uaaauugagu      960

gcaucaauga uacaaagaua ugaagaacac gcaaaagauc uaaaguuauu aaaacgcuuc     1020

guaaaagagc acauaccaga caaauacgcu gaaaucuuca acgaugccac aaaaaacggc     1080

uacgccgguu acauagaugg gaaaaccaaa gaagaagaau ucuauaaaua uuuaaaaacu     1140

acucuaguuc aaaaaagugg auaccaauac uuuauagaaa aaauagaaca agaaaauuuu     1200

uuaagaaaac aacgaauuua cgacaacggu gugauaccac accagguaca ugccgaagaa     1260

cugagagcaa uucucaggaa acaagaaaaa uacuacuccu ucuuaaaaga aaaccacgaa     1320

aagauugaac agauauuuaa aguccguauc ccguauuaug uagguccucu agcaaaacac     1380

aaugaacaau cacgcuucgc cuggaacauu agaaaaucag acgaacccau uaggccaugg     1440

aauaugaaug acgucguaga cgagaacgca agugcaguug cauucauaga aaggaugaca     1500

auaaaagaca uauaccuaaa cgaaaacgua cugccccgcc acaguuuaau auacgaaaaa     1560

uucaccguuu uuaacgaacu aacaaaaguc cucuaugcag acgauagggg aguauuucaa     1620

agauuuucug ccgaagaaaa ggaagauaua uucgagaaac uuuuuaaauc agaaaggaaa     1680

gugacaaaaa aaaagcuaga aaauuauuug cguauagaac uaucuauuuc gucaccuagc     1740

gucaaaggua uagaagaaca auucaaugcu aauuucggca cauaucucga cuuaaaaaaa     1800

uuugacgagc uccauccaua ucuagacgau gaaaaauauc aagauaccuu ggaagaagua     1860

aucaaaguac uuaccguuuu cgaagauaga ucaaugaucc aaaaccaacu agaacaacuc     1920

ccuuuaaacc uaagcacaaa aacaaucaaa gcauuauccc gacgcaaaua uacaggaugg     1980

ggaagacuau ccgcgcgccu aaucgauggu auccacgaua aaaauagcgg uaaaacaaua     2040

uuagacuacc ucauagaaga cgaauccgau ucauauaucg uaaauaggaa cuucaugcaa     2100

uuaauaaaug acgaucauuu aucauuuaaa aaaauaauag aggacucaca accuuacaaa     2160

gaacaacaaa gcgccgaaga aauuguuucc gaacuauccg gcaguccagc caucaagaaa     2220

ggaauuuuac agucuuuaaa aauaguugau gaacuaguug caauaauggg auauaaacca     2280

aaaaauauag uuguagaaau ggcucgggaa aaccaaacua cgggaagagg aaaacagaac     2340

uccaaaccaa gauuaaaagg aaucgaaaau ggacuaaaag aguucucgga uaguguacua     2400

aaagguucca gcauagauaa uaagcaacua caaaacgauc gacucuauuu auacuacuua     2460

caaaacggua aagauaugua uacaggucau gaacucgaua ucgaccaccu aucaacauau     2520

gauauagacc auaucauacc ucaaucauuu cuaacagaca acagcauaga uaacagagua     2580

cucaccaccu cuaaaagcaa cagagggaag agcgacaacg uaccaucaga agagguuguc     2640

cgaaaaaugg accgcuuuug gcgaaaacuc cuaaacgcaa aauuaauuuc agaacgaaaa     2700

uacacaaauu uaaccaaaaa agaacuuaca gaaucagaua aagcaggcuu ccuaaaaaga     2760

caacucguug aaacaaggca aauaacaaaa cacguagcaa caauacuaga uuccaaauuc     2820

aaugaagauu caaauaaucg agacguucaa auaauaacgc uaaaaagugc auuagugucc     2880

gaguuucgga aaacauuuaa ccucuauaaa gucagagaaa uaaaugaucu ucaucacgca     2940

cacgaugcau accuaaaugc aguaguagcc uuaagcuuac uacgaguaua uccucagcuc     3000

aaaccggaau ucguguacgg cgaauacgga aaaaacucca uucacgacca gaacaaagca     3060

accauaaaaa aacaguuuua cuccaauaua acacgguacu ucgcauccaa agacuacauu     3120

auuaacgaug auggugaaau acuauggaau aaacaagaaa ccauagccca agucaucaaa     3180

acacuaggaa ugcaccaagu caauguagua aaaaaagugg aaauacaaaa aggaggauuc     3240

ucaaaagaaa guauacaacc aaaaggagaa agucaaaaac uuauacgccg aaaacaacaa     3300

uggaacacaa aaaaauaugg aggcuucgac ucuccaguag uagcauacgc gauacuauug     3360

ucuuucgaca aagguaaacg aaaagcacgu agcuucaaaa uaguuggaau aaccauccag     3420

gaccgagaau cguucgaagg aaauccuaua cucuaccuau caaaaaagga uuaucacaac     3480

ccgaaaguag aagcaauucu acccaaauau ucccuauucg aauuugaaaa uggcagaaga     3540

cgcaugguag cgagcgcauc cgaaacccaa aaaggaaacc aacuuauaau accaggacac     3600

uuaauggagc uacuauacca cucaaagaaa auaaucaaug ggaaaaauuc agauucaguu     3660

ucuuauaucc aaaauaacaa ggagaaauuc agagagauau uugaauacau cguugacuuu     3720

ucaucuaaau acauaucggc agaugcaaau uuaaacaaaa uagaaaaaau uuucgaaaac     3780

aacuuccaua aagcauccga acaggagaua gcaaaaucau ucauuaaucu ucucacauuc     3840

acggcaaugg gugcaccagc agauuucgaa uucuucggag aaaaaauacc aagaaaacga     3900

uauguaucaa uauccgaaau aauagacgcu guguucauac accagagcau aacuggacua     3960

uacgaaacca gaguaagacu aaccgaagua uga                                  3993


<210>  5262
<211>  3993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5262
auggaaaaag aauauaccau aggacuagau auagguacca auucuguagg cugggcggua       60

cucacagacg acuaucgauu agucgcacga aaaaugucaa uccaagguga cuccaaucgu      120

aaaaaaauaa aaaaaaacuu cuggggagca agauuauucg aagaagguaa aaccgcacaa      180

uuuagacgaa uuaaaagaac caaccgaaga cgcaucgcca ggagaagaca aagggugcuc      240

gcauugcaag auaucuuugc agaagaaauc cacaaaaagg accccaacuu uuuugcacga      300

cuagaagaag gagauagagu cgaagccgac aaacgcuucg cuaaauuucc aguauuugcc      360

acacuauccg aggaaaaaaa cuaucaccga caauacccaa cuauauacca cuuaagacau      420

gacuuagcaa auucuaaaga acaggcggau auuagacugg ucuaccuagc aauagcacau      480

ugcuuaaaau acaggggaca uuuccucuuu gaaggagaac uagauacgga aaauaccucc      540

gucacagaaa acuaucagca auuucuucaa gcauaccaac aauuuuuucc ugaaccuaua      600

ggugacuuag acgacgcagu cccaauacua acagaacgcu uauccaaagc aaaacgaguc      660

gaaaaaguac ucgcauacua cccaagugaa aaaucaaccg gaaacuucgc ucaguuucuc      720

aagcuaaugg uaggaaauca agccaauuuu aaaaaaacau uugaccucga agaagaaaug      780

aaacuuaauu ucacacgcga cuguuaugaa gaggauuuga acgaauuauu agaaaagacg      840

ucagacgacu augccgaacu auucuuaaaa gcuaaaggag uauaugacgc aauacuccug      900

ucccaaauac ugaguaaaag cgacgacgaa accaaggcca aacucagugc aaacaugaag      960

cuaagauucg aagaacauca acgagacuua aaacaacuca aggaauuagu uagaagagau     1020

uuaccuaaaa aauacgacga cuucuucaaa aaccguucua aaaacggaua ugcaggauac     1080

guaaaaggaa aagcaacaca ggaagauuuu uacaaauuuc uaagaacaga acucgccgga     1140

cuugaggaau cacaaagcau aauggagaaa aucgauuuag aaaucuacuu gcugaaacaa     1200

agaacuuuug caaauggagu aauaccccac caaauacacc ucguagaaau gagagaaaua     1260

auggaccgac aaaagagauu cuaucccuuc cuaaaaggag cccagggcaa gauugaaaaa     1320

cuccucacuu uuagaauacc auacuacgua ggaccucucg cccaagaagg acaaaguccu     1380

uucgcgugga uuaaaagaaa aucuccaucc caaauaaccc cauggaauuu cgcagaagua     1440

gucgacaagg aaaauucagc aauagaauuu auugaaagga ugaccaacca agacacauac     1500

cuaccuaaag aaaagguuuu accuaaacaa ucauuaaucu aucaaagauu caugauuuuu     1560

aacgaacuaa caaaaguuuc auacacugac gaaagaggaa aaagccacua cuucucgucg     1620

gaacaaaaaa gaaaaauauu uaaugaacua uucaaacaac auccaagagu uaccgaaaaa     1680

cagcuuagaa aguuucucga gcuaaacgaa caaaucgauu ccaccgaaau caaaggaauu     1740

gaaaccagcu ucaaugcaag uuauucuacu uaucacgauu uacucaaacu cagcgaccaa     1800

auggauacac uauuagacga uccagauaug acaacuaugu uugaagaaau uauuaaaauc     1860

uugaccauuu ucgaagauag agaaaugaua cgagaacaac ugaaaccgua cgaaacaguc     1920

uuagguuuac cugccauaaa aaaacuagcc aaaaaacacu auacgggaug gggcagguua     1980

ucagaaaaaa ugauccaagg uaugagggaa aaacaaucaa gaaaaaccau auuagacuau     2040

cugauugacg acgacgacuu uccgugcaac cgcaaucgaa acuucaugca acuaauaaau     2100

gaugaccacc uauccuuuaa agaaaccauc gccaacgaau ugauaauguc agacucuaau     2160

guccuccuag aucaggucaa agcuauaccc ggaucaccag caguaaaaaa aggaauaugg     2220

caaagcauaa aaauaguaga agaaaucaua gggauaauag gcaaagcacc aaagaauaua     2280

gucauugaaa uggcccguga aaaucaaaga acuucacgcu caagaccaag auuaaaagcc     2340

uuagaagaag cucuaaaaaa uauugacagu ccccuccuaa aagacuaccc uacagauaau     2400

caagcacuac aaaaagauag acuauaucua uauuaccuac aaaacgggaa agacauguac     2460

acuggagaac cacuagaaau ucaccgacua agcgaguaug acauugauca uaucauacca     2520

agaucguuua uagucgacaa uucgcuagac aauaaaguac uugucuccuc caaaguaaac     2580

cgaggaaagu uagacaacgc ucccgacccg uuagucguga agcgaaugag aucacauugg     2640

gaaaaacuac accaagcaaa auugaucuca gacaaaaaac uagcuaaccu uaccaaacaa     2700

aacuuaacug aagcagauaa agcucgcuuc auacaaagac aguuaguaga gacaagacaa     2760

auaacuaaac auguagcaaa ucuacuccau cagcauuuua acuugccaga agaaguguca     2820

gcaacagaaa aaaccucaau aauaacauua aaaaguacac uaacaucaca auuccgacaa     2880

auguuugaca uuuauaaagu acgcgaaaua aaccaccacc aucacgcuca cgaugcuuac     2940

cuaaacggag ucguagcuau gacauuacuc aaaaaauauc cgaaacuugc accagaauuc     3000

guauauggaa guuacaucaa aggagacauu aaccaaauua acaaagcuac agcaaaaaaa     3060

gaauucuacu caaauauaau gaaauuuuuu gaaagcgaag agaucauaug cgaugaacaa     3120

ggggaaguga ucuggaacaa aaaacgcgau cuaucuacca uaaaaaaaac cauaggcgcc     3180

caccaaguca acauaguaaa gaaaguugaa aaacaaaaag gaggauucua caaagaaaca     3240

auaaauucaa aagcuaaucc agaaaaacua auaccuagga aagcaucacu agacccauua     3300

aaauacggag gauacggauc accacucguc gccuacacag uuauuuuuau uuuugaaaaa     3360

ggaaaacaaa aaaaaguaac uaaagguaua gaagguauaa cuguaaugga gcaauuaagg     3420

uucgaacaag accccagaga auuccuuaag acgaaaggcu augaaggcgu uaaacaaugg     3480

cugauacuuc caaaauacau ucuauucgaa gcccagggag gauacagaag gaugauagcu     3540

ucucaccagg aaacacaaaa agcaaacucg cucauacuac cugaaaaccu aguaacccuu     3600

uuauaucaug cuagacauua cgacgaaaua aaccacaaag ucucauucga uuaugugaau     3660

gcccacaaag aaggauuuaa ugacauauuu gacuucaucu cggacuucgg gguacgauac     3720

auauuagcac cucaacauuu agaaaaaauc aaaguagcuu augaaaaaaa caaagaagua     3780

gauuuaaaag aaaugaucga cgcuauacua agcuuauuaa aauuuacauu auuuggagca     3840

ucaguagaau ucaaauuuuu ugauauaaaa auacuaaaac gguacaaauc auuaacagau     3900

aucugggagg cuacaauaau uuaucaauca gucacuggac uauacgaacg aagaguugaa     3960

guucgcaaac uaugggacgg agagagacua uga                                  3993


<210>  5263
<211>  3996
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5263
augaauaaac aacccuacaa cauaggacua gauaucggua ccaguagcgu cgguuggagc       60

gcgaccgaac uagauaaccg uuuguuacac auaaaaggac acaaugggau uggcguacga      120

uuauuuaaag aaggcgaguc agcugcugac agacgcggau ucagaaccac ccguagacga      180

cuugcacgac gaaaauggag acuacgauua uugaaugaaa uauucgccac agaaauagcc      240

aaaguggacc caucguucuu ugcgagauua aaacaaucaa auguaagucc uaaagaucca      300

aacaaaacua uguucgaaaa cauacuauuc gaugacgaaa aucuagauga ccaaaaauuu      360

caccacgacu acaagacaau auaucauuua cgacaagcaa uuauaaacca uccagaacag      420

aaauucgaua uacgcuuaau uuaucuugca augcaccaca uaaucaagua uaggggacau      480

uucuuaaauc aagcuaaugu gcaagacuuc aagggaggga aaauagaccu agaaaaaagu      540

uucaaagcac ucaacgauau auuugagaac caagguaggg acuuaaggcu aguuacggau      600

aaagcgaauc aauauguuaa agugcuauca gacaacgcga aaacacguag ugacagaaaa      660

aaagaacuau ccaaacucuu auacguccca auagauaaag acacugacaa aaauucgaaa      720

aaaguuaccg gcgaaauacu gaaggcaaua cucggaaaca aagcuaaauu cgaugugaua      780

uucggaauag aaguagaaaa ucccaaagaa uggguacuga cauuuaacag ugacgauuuu      840

gacgacaaaa uagaaggacu uagcucccaa augacagacg aagauucaga aauccuucua      900

auacuaaagg aacuauacuu cgcgcuaaau uuaucugaua uacuaaauaa caguguaaca      960

gacaaaaacg caaaaacauu aucugaagcu augauaaaca gauacgauga ucacaaaaac     1020

caacucgcaa ugcuaaaaaa aguaauagau uccuccgaua aaaaacacgc caaggaacuc     1080

aaagaagcau acuccgcaua cauagacgga gaaaacaaca aaaaaauauc auucgaggac     1140

uuaaaaaaac gaauccaaaa aaaucuagua gacagcgaau uaucccaaaa aauuaaugac     1200

cuauuagacc augacuauuu cuuaccaaaa caaagaucaa aagaaaaugg agcaauaccc     1260

caccagcugc aacaacaaga acuugaccaa auaauagaaa gccaaaaaca auacuacccc     1320

uuccucgcug aacuaaaccc gaaugaaaag cgcucaagac aagcaaaaua uaagcuagau     1380

gaguuaguug cauucagagu cccauacuac gugggaccga ugguagaagu aacugauuca     1440

aaccaagaga acaauggaaa auucgcgugg augauaagaa aagaacaagg cgaaaucaca     1500

ccauggaacu uugaugaaaa aguagauaga acugaaacug caacgaaauu uauuaccaga     1560

augacaacca cugauacuua ccuaauaggc gaacccguuc uacccaaaaa uucauugcua     1620

uaucaaaaau ucguaguccu uaacgaacuc aauaaccuaa aaauaaacca ggaaauaaua     1680

acuccaggac aaaaacaaua ccuauacgaa cacgucauga aaaaaaacaa aagaguuaca     1740

uuaaaaaaaa ucgccgaugc auugguaaua aaaggugacu auccauacgc accagaagua     1800

aagggaacaa cagaucaaaa aaauuugaac aacggauuaa ccacauauau agaccuagca     1860

aaaauauuug gaaacgaauu agacgacguc gaccgggaag cagauuucga gaaaauuaua     1920

gaauggucua ccaucuucga agaccaacau auauuugcac uuaaauuaca agaacuaaaa     1980

uggcucacag aaaaacaaag aaaucaaguc guaaaacucc guuaccaagg gugggguaaa     2040

uuaaguaagg aauuauuagc uggauuaguu gacgauaacg gccaaaggau aaucgaccua     2100

cuauggucgg auaaccagaa cuucaugcaa auaguaaauc aaaaaagcuu uaaggaaagu     2160

auagcagauc auaauggugc acaccuggaa gaaaaaaacc uaaaagacgu aauuaaugau     2220

uuauauacgu cuccucaaaa caagaaagca auccgucaag ucauucuggu cguagaugau     2280

auaauaaacg caguuggcua cgaacccgcc aacauuauga uggaauucgc ccgugaagau     2340

ucaaacgacc accgucuaac aaacagccga ucucgacaac ucgaaaacgu auacaaagac     2400

aucacuaacu caugguuuga uaacgacuca guaaaagaug aacuagagga uaaaauaaaa     2460

aacaaagaca aauucacuga ucgacuguac cucuacuuca cccaaggcgg uaaagaucuu     2520

uacacuggag aaccacuuaa cauugaugac cucucauccu augacauaga ccacauacuu     2580

ccccaaucau uuauauuaga uaacucgcuu gacaacagag uuuuaacauc gcaaagaaac     2640

aaccgcguau cuaaagaaga ccaauuacca ggugaacuau ucggaacaaa aaugcaugca     2700

uucuggaaaa gacuaaaagc ugcacaucua auaucaaaca aaaaauauua uaacuugacc     2760

cuaacaccua auagcauaag caaauucaau aaucaagggc gauucaucaa ucgacaacuc     2820

gucgaaacaa gacaggucau aaaguuagca gccgaaauac uccauaacag auauagugaa     2880

gaaaaaggaa caaacauagu aacuauaaaa gccaacuuaa cccaccauau gagagaaaaa     2940

uucaauuuuu acaaaaacag aaacgucaau gacuaucacc acgcuuucga cgccuaccuu     3000

acagcauucg ugggaaacug gcuacuacag caauacccua aauuaaaacc auacuucgua     3060

uauggcgauu uugcgaaaac agaaauuaac aauuuaaaau ccuuuaacau gcucuacaaa     3120

uuugaacaag cccaucaauu aaacgaaaaa gacggaaaaa uagcaaagga cgcagaugca     3180

cuccuaggau auaugaaaaa cguauaucau uuuaaaaaaa uguuaguaac aaaaaaacua     3240

gaaacaaacc acggcgcccu auucaaacaa acacuauacc caagcccagc acacgauaua     3300

aaaaaacggc aauuaauaaa accuaaagac aaccgcccua cagauauuua uggaggauau     3360

acacaugaag aacggaaaua uaugucauua guuaaaguaa uaggaaaaaa agauaacgaa     3420

uaccgaaugg ccaauguccc acuccuaaaa auuaacaaaa auaacgauau aaaagaaauu     3480

cuaaaauccc aguuuuccaa caagaaauuu gaaauagugu uacccaaagu auuaaaaaac     3540

caacuguuca gcgaaaacgg agccuuauuu guaauaggau cuaaaggaua uaaauacaac     3600

gccaaccaau uagugcuauc cgacuuagac augaagcuac uaaacgaccu gagcaacaac     3660

aaaucuaucg augagguaac uaucaaucaa cuauuuaaaa acauugugga cagcacaaau     3720

aaauacaugc cguuauauga gauaaaaaac uuaaaugagg ucguagaaaa auuuaauaau     3780

cucgaauuua acgacaaaaa aaaaacaaua acagauuucc uauuagccac acacgccaac     3840

ccaacaaugu caaaccuaaa aaauaucggc cuccccagcu acuucggacg cuucacaagc     3900

gguaacgcag gcauuaaauu aagcgaaaac gcaaacguaa uuuaucaaag uccaaccggc     3960

cuauuuucaa gaaaaguuaa aauaagcgac uuuuga                               3996


<210>  5264
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5264
augaaaaaca gagaaaauau aaaauauuau aucggaguug auauaggaac uaauagugua       60

ggcugggcag uuauugacga aaauggaaau cuacuaaaaa aaggaaaaca ucacuuaugg      120

gguucucgcc uuuucgacca agcccaaacc gcacaaaaca gaagaaauua uagaaguucu      180

agaagaagau auaauaaacg ccgacaacga auuggacuac ugcgucuaau uaugucugac      240

augguacugg aaguagaucc aagcuuuuuc aucagacuag aaaaaaccac auuucucgau      300

aaagaagaca aaaaagccau ccuaaaagac aauuacaaaa ugaacuauaa ucuuuucugu      360

gacgaagacu auaacgauaa gaaauacuuu aaagauuacc ccacuauuua ucaucuacga      420

aaaaaacuau gcgaauccga cgaaaaggcu gacccccgau uaauauaccu agcccuucac      480

cauauuguaa aguacagagg caacuuucug uacgaaggac aagaauuaca ucuggaaccu      540

ucaaacaagg aagaagaccu caaaauauua uuugauauac uagguaagaa uaacgacaca      600

guuuacgaca ucucagagga acagauucag uuuauacuua aaaccgucgu agaaaacaua      660

ucaaaaaccg cuaaaguaga cgagugcaug ucacaacuaa agcuaaacuc agaagacaaa      720

aaaauaguua aagaauucau gcgaggguug guaggcaaca aauucaacgu aucaaaauua      780

uacaugcaug aggaccuuca auuugaugac gaagaucuua aacuacaguu cuccgacaaa      840

uccuaugaag aaaaaauaac agaauacgaa aauguccuag aagaaaaaau ggaguucauc      900

gaucuaaugc aaagguuuua cucauggauc gaacuaucca agaucguagg aucagacucc      960

caacacgcau caaucagugg cgccaugguu aacaucuaug aaagccaccg ugaagacuug     1020

agaacacuaa aagaagugau guuaaaaaua ggaaaaaaag aauaugauga gauguuuaaa     1080

ccaacuucga agaaugucgu aaacuauuac aacuauguca acccgguagc augcagugga     1140

gacaaaacgg auggauuuua caaauacgua aaaaaagcaa uagaaaaauc cgaugauuca     1200

cgaaaagacg aaauacucca aaaaauagcc aacgaaacgu acauguuaaa acaaacaagc     1260

aaaaauaaug cguacauucc auaucaaaug caaaaagacg aacucaucaa aauacuagac     1320

caucaagaaa aauauuaccc aguauugaaa gaaaacagag acaaaauaau cucaauccua     1380

gaauuccgaa uccccuacua cuacggaccg cucgacggua auaaacaauu cggauggcuu     1440

auuaaaaaaa aagggaaaga aaacgaaaga auccuucccu ggaaucauca agaaauagug     1500

gacguacaag aaacugcggc acaauucaua aaaaaauuaa caaacuacug cacauaucuc     1560

ccaauagaaa aaguuaugcc acaaaaauca uuaacuuguu cuauguacga aguacuauca     1620

gaaguaaaua aaaucagaau agacggaaaa cuacuaccca uagacacgaa aaaucguuua     1680

auagaagacu uauucuucaa aagaaaaaca gucaaagaga aagacuuaau aaauuggcua     1740

aaacaaaauc aacuaaccgu aggagagauc acaggauacc aaaaagaaaa agcauuuucg     1800

aguucauuag cuccuuggau agacuuuaaa gaaauauuug augaaauaaa ugacucuaac     1860

uacgaucuaa uagagaaaau aaucgaagac augaccauau uuaaugaagg auccauccuc     1920

aaggaacgac ucaagaaagc auacaaccua gaccaaaaua aagucaagaa aauaaugaaa     1980

uugaaauacu caggaugguc gcgauuaagc aaaaagcuca uaaacggaau ucgcgcagau     2040

aauaaauuug gauccagcgu aagcauauua gacgucauga aagaaagcca cauggcacua     2100

auggaaauca uaaacgauca agacuugggc uucaaacaaa uaauagaaaa agaaaauuuu     2160

aaaaacgaaa ccgguucauu ugaauacgaa gacaucgaaa auuuagcagg uucuccugcg     2220

cuuaaaagag gaauauggca aacccuucaa guaauagaag aaauaaaaaa cuauaugggc     2280

cacgaaccaa aaaauauuua uauugaauuc gcaagagaag aacaagaaaa aguuagaaca     2340

accacaagag uaaaaaaauu aaaaucaaua uauaacgaca uaaaaaacca auuagacgua     2400

cacggaaaag aaguguauag caaucuaaau aagcaagaug aaaaaaguag cauagaaaag     2460

agacuauauc uauauuacac acaacucggc aaaugcaugu acucaggaga aucacuagau     2520

auagacaaau uaucaaacua ugaaauagau cauaucuucc caaggacccu cacgagcgac     2580

gacagucuag auaaucuagu auugguaaaa aaaaaagaaa accaaagaaa acucgaugac     2640

cucguccugc cacucgagau acggaauaaa auggagguau uuuggaaaaa acuauacgac     2700

aacggacuaa uaacucaaac aaaauacuac cgguuaauga gagacgaauu ucguagagau     2760

caaauagaca aauucaucaa uagacaauua gucgaaacgc gacaaauaau aaaacaugua     2820

gcaaauauaa uugaaaacca cuacgaggac acuaaaguuu uuacgguaag agcuaaucuc     2880

ucccaugaau uucgagaaaa auauggcauu uacaaaaaca gaaacguaaa cgauuuccau     2940

cacgcacacg acgcauacau cgccugcaua auaggcagau auauacaaau aagauucccg     3000

ggacuagaag cuaaauacgu guacggacag uacaugcaaa augugaaaaa aacaaaaaac     3060

aacauagaca aggaaaacca cggauuuaua auaaacucaa ugaaauauga augcauugac     3120

gaagacacag gugaaguuau cuggaaccca gaacgaauac ucgauuauau aaaauguuuu     3180

aacuacagag acgucuacau cacaaaaaag cuagauacga acaauagaga auuauuuaau     3240

guuacaauuu uaccaaauga uaaaaacucu gaaaaaggua aaacaaaagc gacaaucccc     3300

guaaacaaau uaagaagcaa cgucagaaaa uauggaggcu uuucaggacu ccaguccgac     3360

auaguagcaa uagaaggaaa aaaaggcaaa aagaucgaua ggcgucuaau aaaccuaccg     3420

auucugcuac gauacguguc aauugaagac caaugcaaau auauaaugga gaauaauaaa     3480

uacagugacg uaaaaauaau aaaaaagaua aaaaagaacc aacuaauaga aauagauggu     3540

auuuguuacu aucuaaaauc ggcagacgaa cucgauacag cauaccaauu gauucucagu     3600

agagacgaca acgagaugau auaccgaaua gacagagcua uccaaaacaa ugauuucucc     3660

uauuucgaca acaaaaaaga aaauauagac gagcuguaca gacuaauaug ucauaaaaug     3720

aucgaaaacu auccaaaaua caagaaaaaa guuuacgagu uuauaaaaga gcgcgaagag     3780

gaguucuucg aauuagauaa uagucaaaaa uguaucguca uauucgaaau gauaagaguc     3840

augaaacgag gcgagcaaua cauaaacaua ccuuucgaac cuuacaaccu uaagaaugac     3900

agaaaaggaa gacuaaauug ucaaagcauu cuguuagaua auacuuacuu cuaugacguu     3960

ucaaucaccg gcauauauuc uaagaaguau aaauuauga                            3999


<210>  5265
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5265
augcagaaaa auuauacaau aggacucgac auaggaacaa acucaguagg augggccguu       60

augaaggacg auuauacacu aauaagaaaa cgaaugaaag uucuaggaaa uacugacaua      120

aagaaaauaa agaaaaauuu uuggggcgua cgcuuauucg acgaaggcga aacagcgaaa      180

gaaaccagac uaaaaagagg aacacgaaga agauaccaaa gaagacguaa ccgucucaua      240

uacuuacaag acauuuucca acaaccaaug cuagcaauag augaaaacuu cuuccaucgu      300

cuagacgacu cauucuuugu gccugaugac aaaaguuacg acagacaucc aauuuuuggc      360

ucauuagaag aagaagucgc cuaucauaac accuauccaa ccauauauca ccuacgcaaa      420

cauuuagccg acaauccaga aaaagccgac cuaaggcuag ucuacacagc ucuugcacac      480

auagucaagu accgaggcca cuuucuaauc gaaggggaac ucaauacaga aaauacaagc      540

aucucagaaa cguucgagca auucuuagau acuuacucag acauauuuaa agaacaauua      600

gugggcgaca uaucaaaagu cgaagaaaua cucaguagca aacaauccag gagccguaaa      660

cacgagcaaa uaauggccuu auucccaaau gaaaauaaau uaggcaacuu cgggagauuu      720

augauguuaa uuguuggcaa uaccaguaac uucaaaccag uuuucgauuu ggaugacgag      780

uauaaacuaa aacuauccga cgaaacuuac gaagaagacc uagauacacu acuggguaug      840

acagacgacg uauuucuaga cguauuuaug gcagccaaaa auguguacga ugcaguagaa      900

augucagcua uaauuaguac ugacacagga aacaguaaag cgguucuauc uaaccaaaug      960

aucaauuuuu acgacgaaca uaaaguugau cuagcucaau uaaaacaauu cuucaaaaca     1020

cacuuaccug auaaauacua cgaaugcuuu ucagauccaa guaaaaacgg uuacgcagga     1080

uauauugaug gaaaaacaaa ucaagaggac uucuacaaau acaucgaaaa aguaaugaag     1140

acgauaaaau cagauaagaa agacuacuuu uuagauaaaa uagacagaga gguuuuucug     1200

aggaaacaac gaaguuuuua uaacagcgua auaccacacc aaauucaucu acaagaaaug     1260

caagcaauac uagacagaca aucccaauau uaucccuucu uagcggaaaa ucgugacaaa     1320

auagaaucuc ugguaacauu cagaauacca uauuaugucg ggccacuaac aguuagcgac     1380

caaucagaau uugcauggau ggaaagacaa uccgaugaac caauaaggcc auggaacuuc     1440

gacgaaauag uuaauaaaga aagaagcgca gagaaauuca uagaaagaau gaccaacaug     1500

gacaccuacu uauuggaaga aaaaguauua cccaaaaggu cacuccucua ccaaaccuuu     1560

gaaguauaca augaacuuac caagguacgu uacacaaaug aacaaggaaa aacugaaaaa     1620

cuaaacaggc aacaaaaagc cgagauuauu gagacccuau ucaaacagaa aaaccgagua     1680

agggaaaagg acauagcaaa uuaccuagaa caauacggcu acguggaugg cacugauauc     1740

aaaggaguag aagauaaauu caacgcaagu uuaaguacuu acaaugaccu ggcaaaaauc     1800

gauggggcaa aagcauaccu agaugacccu gaauaugcag acguauggga agauauaaua     1860

aaaauauuaa caauuuucga agauaaagcc augagaaaga aacaacuaca aacauacuca     1920

gacacccucu cgcccgaaau acuaaagaaa cuagagcgaa aacauuauac gggaugggga     1980

agauuuucca aaaagcugau caacggauua agagacgaag gcagcaauaa aacgauacuc     2040

gauuacuuaa aaucugauga aggaucauca ggacccacaa acagaaacuu uaugcaauua     2100

auacgggaua acacucucag cuucaaaaag aaaauagaag acgcacaaac aauagaagac     2160

acuacgcaua ucuaugacac cguugcagaa cuaccaggaa guccugcgau aaagaaaggc     2220

auucgacaag cucuaaaaau aguugaagaa auaauagaca uaaucggaua cgaaccagag     2280

aacauaguag uagaaauggc ucgagaauca caaacaacca agaaaggaaa agaccuauca     2340

aaagaacguu uagaaaaacu aaccgaagcg auaaaagagu ucgauggacc uagugauguc     2400

aaagugaaag auuuaaagaa cgaaaaccua agaaacgacc gccuauauuu auauuaccuu     2460

caaaacggac gagauaugua cacaaaugaa ccgcuagaca uaaauaauuu aucaaaauac     2520

gauauagacc acauaauucc ucaaucauuc acaacagaua acucuauaga caacaaaguc     2580

cucguuagca ggacuaaaaa ucaagggaac aaaucugaug auguaccuuc aaucaacauu     2640

gugcauaaaa ugaaaccauu cuggcgacaa uuacacaaag cuggucugau aucagaucgc     2700

aaauucaaaa aucuaacaaa agcagaacac ggcggcuuga cagaagccga cagagcgcac     2760

uuccuuaaca gacaauuagu agaaacaaga caaauuacaa aacauguagc caaucuguua     2820

gacagucaau acaauacagc agaagaacaa agaaucaaua ucgugcuuuu aaaauccucu     2880

augacuucaa gauucagaaa agaguucaaa cuauacaaag uucgagaaau aaacgauuau     2940

caccacggcc augacgccua ccuuaaugca guaguagcaa caacaaucau gaaaguauac     3000

cccaaucuua aaccccaauu uguauacggg caauacaaga aaacauccau guuuaaagaa     3060

gaaaaagcua cagcccggaa acacuucuac ucaaacauaa caaaauucuu caagaaagaa     3120

aaaguaguaa acgaagaaac aggugaaauc cuaugggaua cagaacgcca uuuaucaacu     3180

auaaaaaggg uuuuguccug gaaacaaaug aacauaguga agaaaguaga aaaacaaaaa     3240

gggcaacucu ggaaagaaac aaucuacccu aaaggugacu ccuccaaauu gauaccaguu     3300

aaagaaggaa uggacccaca aaaauacgga ggauuaaguc aaguaucuga agccuuugcc     3360

guaguaauca cucacgaaaa aggaaagaag aaacaauuaa aaagugacuu aauuucuauc     3420

ccaauuguag accaaaaagc auaugaacaa caccccacag cauauuuaga agaagcugga     3480

uauaacaacc caacuguacu acaugaauua uucaaauauc aguuauuuga auuagaagau     3540

ggaucacgac gaaugauagc cucagccaaa gaauuucaaa aaggcaacca aauggucuua     3600

cccuuggaau uagucgaacu gcuuuaccac gcuaacagau acgauaaagu uaaauucccu     3660

gacagcauag aauacguuca ugauaaucua gccaaauuug acgaucuucu ggaauaugua     3720

auagacuuuu caaacaaaua cauuaaugcu gauaaaaaug uacagaaaau ccagaaaaua     3780

uacaaagaac acggaacaga agaugucgaa uuaacaguag aaucauucgu aaaccuaaug     3840

acauuuaccg cuaugggcgc accagcuacu uucaaauuuu acggagaauc aauaacaaga     3900

ucuagauaca cuucaaucac ggaguuccga ggaaguacac ugaucuuuca aucaauaaca     3960

ggucuauacg aaacccgaua uaaacuagaa gauaacuga                            3999


<210>  5266
<211>  3999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5266
augaugaaag aguauaguau cggauuagac auaggcucua guagcguugg auucguggca       60

guagaugauc aauaccgagu agugagagca aaagggaaaa acgucaucgg caccagacua      120

uuugacgagg gcaaaacagc agcagaucga agaggauugc gaacaacgcg ucgcagauua      180

uccagaagga aauggagauu gagacuccua cgggggaucu ucgaaccgca uauaaacgaa      240

auagaugaau ccuucuuuau gcgguuaaaa gaauccaaua ucucgccgaa agauucaaac      300

aaacacuuuc caggagacau auuauuuaau gauagaucag aucgagaguu uuacgagaau      360

uacccaacaa uuuaccaucu acgacaggca cuaaaaacug agaaaagaca auuugauaua      420

cgcgaaauau accuugcaau gcaccacaua gugaaauaua gggggcacuu uuuggagggc      480

ggaacgguga cagacuuuga agucggcgaa cuugacuuag cgggaaaguu uagcagacuc      540

aaugaacuau uugaaaguau aaaucuagaa guucccuucg uuuuaagacu agacaaucua      600

gaagacguaa aagcaguucu uauagacuca caacguagug cccaagaucg acaacgaacg      660

cuaacaccag auauuuacgu accauccaca gacaaaauca cagagaaaag auuuaaagga      720

auagcaacgg aauuauuaaa agcaaucguc ggaaauaaag cuaaauuuga cguuuuagca      780

ggauuagaac uugacaaaga agaagcaaaa uuaugggcac uuacauucga uucggaaacc      840

uuugacgaag acaucgaaca acuagaagga gauaugacug aagcaacgca agaccuaaua      900

gaaauucuaa aaucauuaag aucauuaaua agauuagcag uaaacaugcc agaaggagua      960

ucauuaagcg aaaugaugau acaaagauau gaagaucaug cagaucacuu aaaauugcuc     1020

agagacauua uacacaauac ugaugcugaa aaagcagccg gauugcaagc agcuuacgau     1080

ggauacauug auggaguaaa agguaagaaa acaccaguag acgauuuuua uaaagcuaua     1140

aagaaaaauc uggaugauag cgaggcagca caccaaaucc uggcguuaau agauaaaaac     1200

gaguucaugc ccaaacaaag gucgaaaagc aauggcugga uaccccauca aauacaacaa     1260

aaagaauuag auagaaucau agaaaaccaa aaacaauacu auccauggcu ggcagaugua     1320

aaccccaaug aaucaagaag aaaaguugca ccuuauaaaa uagacgaauu gguaacauuc     1380

aggguaccau acuauguagg ccccuuagug accagugaag aacaaaagaa auauggcuca     1440

aaauucgcau ggaugguacg aaaagaagaa aaaggugaaa uaacuccuug gaacuucgau     1500

cagaaagucg accgugccgc aagcgcuacu gaguucauua acagaaugac aacuauggau     1560

acauaccucu uaaaugaaga uguauuaccc gcgcauucau uaauuuauca aaaauaugaa     1620

guccuaaaug aacuuaacaa agugaaaaua aauggagaug ccauaacacc cgaauugaaa     1680

cagaaaauau accaugauuu auuugaaaaa caaacaacag uaagcaucaa aaacuuacaa     1740

acauauuuag uaucagaauc cgaagcaagc gaaguaccag aaauaaaagg acuagcagac     1800

gaaaagaaau uucuuagcgg ucugacaacg uaucacgacc uaaugaaaau auucgaccgu     1860

aacguaguaa acgaucccga acgugcagaa gauuucgaga aaaucauaag auggucuaca     1920

gucuucgaag acaaacacau uuauagagaa aaauuagauu cauugagcug guugacaacu     1980

gaccaaaaag auaggcucgc aguaaaacgg uauagagggu ggggaagacu cagcgaaaaa     2040

uuacuaauag gauuaagaga ugaaggacac cgaucaauaa uggacuauuu augggaaacc     2100

acuagcaauu ucaugcagau ccaggcacaa aacgauuucu cggagaaaau ugcagcagaa     2160

aauaaagaau uuuuagauaa uacacaaagu gugcaaggua uaauaaauga auuauacaca     2220

ucgccacaaa acaagaaagc cauaagacaa acauuauuag uaguggaaga cauuaaaaag     2280

gcauuccacg gagaagcacc aaaaagaauu uuuguagaau uugcucgaga aaacgaaagu     2340

acaccacaaa gaucaauaca aagacaaaga caaguagaaa augcauacga aaaaguuacc     2400

caaaauuuac ugguagauga caaaaucaga gcugaauuaa aggacagcau aaaugagaaa     2460

agguuauuuc augaucgacu cuuccuauac uuuaugcaag gaggcauaga cauguauagc     2520

gguaagaaaa uuaauauaga caaccuaagu gcguaugaca ucgaccauau acuaccacaa     2580

ucguucacag uugacaauag ucuugacaau agaguccuua caaaucagaa agacaaccga     2640

gaaaaaggua auucucuucc aagcgaauua uauagaggua gcaugaaaga aaccugggaa     2700

augaugagga aucaaggacu acuaucaaaa gguaaguucc accgauuaau gaugacacca     2760

aacaguauga cagccuauga ccaacaaggc uucaucaacc gacagcuagu agaaacaaga     2820

caagugauua aacucgcugu cgaaauucuu gcauccgaau uugaucaagc agacgagaaa     2880

guagaaauag ucagugucaa agcgggucuc acacaucaaa ugcgacaaga cuuuagccua     2940

auuaaaaaua gagacguaaa ugacuaucac cacgcauuug acgcauaucu aacagcauuc     3000

guagggacau acuuauugaa gagauaucca aaacuacgac cauauuuugu cuauggugaa     3060

uuuaaaaagu uuagucaaaa gcaagcaaau uuaaccaacu uuaacuuuuu augggcguua     3120

aaacauaagg accaaguagc uguagaagau acaggcgaga aaauauggaa uaaaccccag     3180

gauuuagcgu acauaagaca ucuuuuugca uuuaagaaaa uacuaauuuc aagagaagua     3240

ucagaaaggc acggagcacu auuugaucaa acacuauaca aagcgucaga ugauaaagca     3300

ucuggacaag gcucgaagaa acuaauaccu gcaaaagaua acagaccaac cgaauuguau     3360

gguggauauu cuggacaaac auccgcauuc auggcaauag uaaagguaac caaagguaaa     3420

aagacaaaau accuaguagu uggaguacca acagcagcua uuagucaacu acagggaaug     3480

gauguaaaaa gcucacagua caccgaaaag auauauaaua uacuaagucc aaaguuauua     3540

ucuaaaaacg augauuucga agugauaguu ccaaaaguua gauacagaca acuaauauua     3600

gauaacaacc aauuauucac uuuaggauca gacacauaua aauacaacgc gcgccaacuu     3660

guccuuucua acgaaguauu agcagugaua aaucgugaua aauccuuuau agauaagaaa     3720

cuaucagaag aagcagaaaa ccaagcacua aacgacgccu uugaugaaau agucguagca     3780

auggacaaac acuuugaccu auacgauaaa aacggcuuua gagaaaaagu aaaagcgggu     3840

aaacaaaggu ucgaagaacu ccccgugucc uuauucucag aagacaaaga aaaugaagga     3900

auuacaaaac gcacagucuu aaaaaggauu cuacaaggau uacacgcaaa cgcaaccaca     3960

ucugauuuaa aaguacuaga cggaaaauuc uauaauuga                            3999


<210>  5267
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5267
augaaaaauu auacaaucgg ucuagauaua gguguagcgu caguugguug gguaugcaua       60

gacgaaaacu acaaaauuuu gaauuacaac aaucgccacg cuuucggagu acaugaauuu      120

gaaucagcag aaucagcagc aggaagacga cuaaaaagag guaugagacg aagauacaau      180

agaagaaaga aacguuuaca auuauugcaa ucguuauucg acucguacau aacagacuca      240

ggguucuuua guaaaacaga uagccaacau uucuggaaaa auaauaauga guucgagaau      300

cgaucacuua cggagguucu uagcucgcuc cguaucucuu caagaaaaua ccccacaaua      360

uaucacuuaa gaucagauuu aauugaauca aacaagaaaa uggaucuuag auuagucuac      420

uuggcucuac auaacuuagu gaaauauaga gggcauuucc ugcaagaagg aaacugguca      480

gaagcagcga gugccgaagg aauggacgau caacuacuag aauuaguaac cagauaugca      540

gaacuagaga aucucucacc acuugaccuc ucagaaucgc aguggaaagc cgcagagacu      600

cugcucuuaa aucgaaacuu aacaaaaaca gaucaaucaa aagaacugac agcgauguuc      660

ggcaaagaau acgagccauu uugcaagcua guagccgggc ucggggucag cuuacaucaa      720

uuguuuccgu caagcgaaca agcgcuagcc uacaaagaaa caaaaacuaa aguacaacuc      780

agcaaugaaa augucgaaga aguaauggaa cuauuacugg aagaagaauc cgcauuacuu      840

gaagcuguuc aaccauucua ucaacaagug guauuauacg aauuauuaaa aggcgaaacc      900

uacguugcaa aagccaaagu cagcgcguuc aaacaauacc aaaaagacau ggccagucua      960

aagaaccuac uagauaaaac uuucggagaa aaagucuacc guagcuauuu caucucagau     1020

aaaaacucuc aacgagaaua ccaaaaauca cacaaaguug aaguauuaug uaaacuagau     1080

caauuuaaua aagaagcuaa auuugcagaa accuuuuaua aagaccuaaa gaaacuacua     1140

gaagauaaaa gcaaaacaag uaucggaaca acugaaaaag acgaaaugcu gagaauuaua     1200

aaagcaauag acaguaacca auucuugcaa aaacaaaaag gcauacaaaa cgcagcaaua     1260

ccgcaucaaa acucacuaua cgaagcagag aaaaucuuga gaaaccaaca agcacacuac     1320

ccguucauaa caaccgaaug gauugaaaaa gugaaacaaa uacuagcuuu ucguauuccc     1380

uauuacaucg gcccucuagu gaaagauacu acacaaucac cauucuccug gguagaaaga     1440

aaaggugacg ccccaauaac uccauggaac uucgaugaac aaaucgacaa ggcugcauca     1500

gcagaggcau uuauaucaag aaugagaaaa accuguacau aucuuaaagg acaagaagug     1560

uuaccaaaau caucacugac auaugaaagg uuugaagucu uaaacgaauu aaacggcauu     1620

caacuaagaa cuacuggagc ugaaucagau uuucgccaca gacuaagcua ugaaaugaaa     1680

uguuggauaa uagauaaugu cuucaaacaa uacaaaacug uaaguacaaa aagauuacug     1740

caagagcuga aaaagagccc auacgcagau gaauuauacg acgaacauac aggagaaauu     1800

aaagaaguuu ucgggacuca aaaagaaaac gcauuugcaa cuagcuuguc aggauacaua     1860

ucaaugaaau caauucuagg ugcaguagua gacgauaacc cugcaaugac agaagaacuu     1920

auuuacugga ucgcaguauu ugaagacaga gagauauuac acuuaaaaau acaagaaaaa     1980

uauccaucaa uuaccgaugu acaaagacag aaacuagcgu uaguaaaacu cccaggaugg     2040

gguagguucu cuagauuacu cauagauggg cuaccccugg acgaacaagg gcaaagugua     2100

cuagaccaua uggaacaaua cagcucagua uucauggaag uacugaaaaa uaaaggauuc     2160

ggauuagaga aaaagauaca gaaaaugaac caacaucaag uggacggaac aaagaaaauu     2220

cguuaugaag acauagaaga acuugcaggg agccccgcac uaaaaagagg cauauggcga     2280

ucaguaaaaa ucguugaaga auuagucuca auuuucggag aaccagcgaa cauaguauua     2340

gaaguagcga gagaagaugg agaaaagaaa cgaaccaaaa gccguaaaga ccaaugggaa     2400

gaacuuacca aaacaacacu caaaaaugau ccagacuuaa aauccuucau uggagaaauc     2460

aagucacaag gugaccaaag guucaaugaa caaagauuuu gguuauacgu cacucaacaa     2520

ggaaaaugcc uauacacagg uaaagcgcua gacauccaga auuuauccau guaugaaguc     2580

gaccauaucu uaccucaaaa cuuuguaaaa gaugacucac uggauaaucu agcccuugua     2640

augccagaag caaaccaacg uaaaaaucaa gucggacaaa acaaaaugcc cuuagagaua     2700

auugaggcua accaacaaua cgcaaugcga acacuuuggg aaagauuaca ugaauuaaaa     2760

uuaauaucuu caggaaaacu agguagacua aagaaacccu ccuucgacga agucgauaaa     2820

gacaaauuua uagcacgaca guuaguagaa acgcgacaaa ucauuaaaca ugugagagac     2880

cugcuagaug aaagguucuc uaaauccgac auacaucugg uaaaagcagg uaucgucagu     2940

aaauucagaa gauuuagcga aauacccaaa auuagagauu auaacaauaa acaucaugcg     3000

auggaugcac uauuugcagc cgcauuaaua caauccauau uaggaaaaua uggcaaaaau     3060

uuccuagcau uugaucucuc aaagaaagac cgccaaaaac aauggagaag cguaaaagga     3120

ucgaauaaag aguucuuuuu guucaaaaau uuuggaaacc uacgacucca aagccccgug     3180

acaggagaag aagugucagg aguagaauau augaaacacg uauauuucga auuaccaugg     3240

cagaccacaa aaaugacaca aaccggagac ggaauguuuu acaaagaauc gauauuuagu     3300

ccuaagguaa aacaagcgaa auauguaucu ccuaaaacag aaaaauucgu acacgacgaa     3360

guuaaaaauc auagcauaug uuuaguagaa uuuacauuua ugaagaaaga aaaagaaguu     3420

caagaaacaa aauuuauuga ccuaaaggua auugagcauc accaauuuuu aaaagaaccc     3480

gaaucccaac ucgcaaaauu ucuugcugaa aaagaaacua acagcccaau aauacacgca     3540

cgcauuauaa gaacaauacc aaaauaccag aaaauaugga ucgaacauuu cccuuacuau     3600

uuuauaucaa caagagaguu acauaaugcc cgacaauuug aaauaagcua cgaacucaug     3660

gaaaagguaa aacaauuauc ugagagaucu ucaguagaag aguuaaaaau aguguucgga     3720

cuccuaauug accaaaugaa ugauaauuac ccuaucuaua ccaaaucaag uauucaggau     3780

cggguacaaa aauuuguaga cacacaacug uacgauuuua agagcuuuga aauaggauuc     3840

gaggaauuaa agaaagcagu agcagcaaac gcccaacgau cugacaccuu uggaucaaga     3900

auuagcaaga aaccaaaacc ugaagaaguu gcgauagggu acgaaucaau uacaggauua     3960

aaauacagaa aaccaagaag uguuguaggu accaaacgau ga                        4002


<210>  5268
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5268
augucacaaa auauaguuga uuauuguaua ggcuuagacu uaggaacugg aaguguugga       60

ugggccgugg ucgacaugaa ccaccgucuu augaaaagaa acgguaaaca ucuaugggga      120

uccagauugu uuagcaaugc ggaaacagca gcaaacagaa gagcaucacg aaguauuaga      180

agacgauaua auaaaagacg ugaacgaauc agacuccucc gagcaauuuu gcaagacaug      240

gucuuagaaa augaccccac auucuuuaua agacucgaac acacaucguu ccuagacgaa      300

gaagacaaag cuaacuaccu cggcgccgau uauaaagaua acuacaauuu auucaucgac      360

gaggauuuca augacuauac auauuaccac aaauacccaa caaucuauca ccuccgaaaa      420

gcacucugcg aaagcacaga aaaagcagac ccacgcuuaa uauauuuagc ccuacaccau      480

auagugaaau auagaggcaa cuuccuauau gaaggucaaa aauuuaauau ggacgcaagu      540

aacauugagg acaggcuguc cgacguuuuc acacaauuug ccgacuucaa uaacauacca      600

uacgaggacg acgaaaaaaa aaaccuagaa auacuagaaa uauuaaaaaa accauuguca      660

aaaaaagcua aaguugacga agucauggca uuaauugccc cugaaaaaga cuucaaaucg      720

gcauacaaag aauuaguaac aggcauagcu ggaaauaaaa ugaauguaac aaagaugauc      780

uuaugcgaac caaucaaaca aggagauucc gaaaucaaac uaaaauucuc cgacucgaac      840

uaugacgacc aauucucaga agucgagaac gauuuaggag aauacguaga auucauugac      900

ucauuacaca acauauauuc cugggucgag cuacaaacaa uuaugggagc gacacauacc      960

uauaacgcca gcaucagcga agcgaugguc ucuagauaua auaaacacca ugaagaucua     1020

caacuacuua aaaaauguau aaaagauaau guaccaaaaa aauacuuuga cauguuccga     1080

aacgacucag aaaaacuaaa aggcuauuac aacuacauaa accacccuag caaagccccc     1140

guugaugaau ucuauaaaua uguuaaaaaa ugcaucgaaa aaguagauac uccagaagcu     1200

aaacaaauuu uacacgacau cgaacuagaa aauuucuuau uaaaacagaa uucacgaaca     1260

aauggaagcg ucccguacca aaugcaauua gacgaaauga uuaaaauaau agauaaucaa     1320

gccaaauacu acccaguucu caaggaaaaa cgagagcaau uacucucaau auuaacauuc     1380

aaaaucccau acuauuuugg acccuuaaac gaaacaucug aacacgcaug gauaaaaaga     1440

uuagagggga aagaaaacca acgaauacua cccuggaauu aucaagacau aguagaugug     1500

gaugcgacag cagaaggauu cauaaaaaga augagaucuu auugcaccua uuucccggau     1560

gaggaagugc ugcccaagaa uagucuaaua guaucuaaau acgagguuua caaugaauua     1620

aauaagauca gagucgacga uaagcuacua gaaguagaca uaaaaaauga uauauacaac     1680

gaacuuuuca ugaauaacaa aacagucaca gagaaaaaac uaaaaaacug guuaguaaau     1740

aaucaauguu guaacaaaaa cgccgaaaua aaaggauucc aaaaagaaaa ccaauucuca     1800

acaagucuua caccuuggau cgauuucacu aauauauuug gugaaauaaa ccaaaguaac     1860

uucgaucuaa uagaagauau aauauaugau cucacuguau ucgaagauaa aaaaauaaug     1920

aaacgcagau uaaaaaaaaa auacgcccua cccgacgaca aaaucaaaca aauauuaaaa     1980

cuaaaauaca aagacuggag cagacuaucu aaaaaauuac uagacggaau aguagcagac     2040

aacaaauucg gcucaucagu uacaguacuc gacguauuag aaaugucccg ccugaaucuc     2100

auggaaauua uaaaugauag ggacuuaggc uaugcucaga ugauugaagc agcugcaagc     2160

uguccagaag acggaaaauu uaccuacaaa gagguccaac gacuugcggg aaguccagca     2220

cucaaaagag gcauuuggca aucucugcaa auaguugaag aaauaacgaa agugaugaag     2280

ugucguccaa aauacauuua caucgaauuc gaacgaucag aagaaacaaa agaaagaaca     2340

gaaucaaaga uaaaaaaauu agaaaacgua uauaaagauu uggacgaaca gacaaaaguu     2400

gaauauaaaa caguacuaga agaacuaaaa ggcuucgaua auacgaaaaa aauuaguagu     2460

gacagccuuu uccuauauuu uacacaacua gguaagugca uguacucagg aaaaaagcug     2520

gauauagacu cuuuagacaa auaccaaaua gaccacauag ugccacaguc acuaguaaaa     2580

gacgacucuu uugauaauag aguacuagua guaccaucag aaaaucaacg aaaacucgau     2640

gaucuaguag ugccaaguga uauuagagug aaaaugaaca guuucuggaa auuacuauuc     2700

gaccaugagc uuauaagccc aaaaaaauuc uacagcuuga uuaaaacaga auacaccgaa     2760

agagaugaag agcgcuuuau aaauaggcag cuaguggaaa caagacaaau aacuaagaac     2820

guuacucaaa ucauagaaga ccauuauucc acuaccaaag ucgcagcaau aagagcuaac     2880

uuaagucaug aauuuagagu aaaaaaucau auauacaaaa aucgagacau aaacgauuau     2940

caucaugcac acgaugcgua caucguagca cugauaggcg gauucaugag agacagauac     3000

ccgaacaugc augauucgaa agcaguauau ucggaguaca ugaaaauguu cagaaaaaac     3060

aaaaacgaca aaaaacggug gaaagaugga uuuguaauaa auagcaugaa cuaccccuau     3120

gaaguagaug gcgaguuaau auggaacccu gauauaauua acgaaaucag aaaaugcuuc     3180

uacuacaaag acuguuacug cacaacaaag cuugaccaaa aaucgggcca aauguuuaac     3240

cuaaccgucc uaccaaacga cgcacacagc ccuaaaggaa ccacagaggc cguaauaccc     3300

gugaacaaaa acagaaaaga cgucaauaaa uacggugguu ucagcggacu gcaauaugua     3360

auaguagcaa uagaaggaaa aaaaaaacga gguaaaaaaa ccaaacuugu aaaaaaaaua     3420

uccgguguac cacuucacuu gaaggcggca ucacuagacg aaaaaauuaa auacauagaa     3480

gaaaaagaaa accuaacaga uguuaaaauu auaaaagaua gcauaccagu uaaccaaaug     3540

auugaaaugg acggaggcga auaccuccua acaucaccca uagaauucgu uaauggccgu     3600

caacuaguac uaaaugaaaa acaaugcgca uuaauagccg acauauacaa cgcaaucuac     3660

aagcaagauu gcgacaacuu agacgacgua uuaaugauuc aauuauacau agaacugauu     3720

aauaaaauga aagcuuugua uccugcauac caauccauag cagaaaaauu cgaaaguaug     3780

acagaagauu acguggcagu uucaaaggaa gaaaaagcag auauaaucaa acaaaugcua     3840

auuauaaugc acagaggucc cagaaacgga aaaauacaau acgcagacuu caacgugggu     3900

gauagaauug gaaggaaaaa caaaaugagc cuggauuuag aaagaguaac uuucguauca     3960

cagagcccua cagggaucua cacaaaaaag uauaaacucu ga                        4002


<210>  5269
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5269
augggcaaag aauacuauuu agggcuagau aucggaacag acuccguugg augggccgua       60

ugugauacaa auuacaaaau accaaaauuc aaagguaacg caaugugggg aauaagacuc      120

uuugaaaaaa guaauagcgc cgaggaacga agaggauuca gaagcagucg aagaagaacc      180

caaagaaaaa gagaaaggcu caaauuauug gagaugcuau ucgacgaaga aauuucaaaa      240

aucgauccag ccuuuuucau caaacugaga gaaagcaacc uuuauuuaga agauaaauca      300

acgaaaaucc cauacugugu auuuucagau gaaaacuaua cagacaaaga cuuucacaga      360

gacuauccca caauauauca cuugcgaaac gaacuuauaa aaucaaauaa accucacgac      420

guaagacuag uauacuuagc acuacaccac aucauaaaga auagagguca cuuccuauuc      480

gacuacuccu auaaaaacga uaaaucagga gauuucaugc caauuuauaa ugacuuaaaa      540

aauuauuucu augacaauua ugaaauugaa cuagauugca gugauguuga cgaauuugcc      600

aauacacuaa aggauaaaag caagggaaaa acaaaaaaaa acgcagaaau ugcagaguua      660

uucggagucu ccaaaaaaua ugacaaaguc cuuuacagcg cacuaucacu ucuaucagga      720

aguucaguaa agcuuuuuga uaucuuugau gaugaggcac uuaaagacgu agaaaaaaag      780

ucaguaaguu ucucaagugg auuugacgau aaugcuaacg acuuucaaag uuacuuaggc      840

gaaagauucg aacuucucga aaaacuuaaa gcaguguacg auugggcagu uuuagcugau      900

auacuggacg gaaaagagua cauaucugag gccaaaguag cuauauacga aaaacauaaa      960

agggaucuaa aaacacucaa aaaauacauc aaaauauaca ugccagaaaa aaaagcugaa     1020

auauucaaua uuaguucaaa agauaacaac uauguggcau acuccaaaca cauaaaaacc     1080

ucaaauggaa caggaguacu cauuagcacc ugcacgcaag aagaauucug cgcauaccuc     1140

aaaaaaacac uuggaauaug caaagacgca agcuaugaag aaauguucaa agaaaucgaa     1200

aacucaaccu uuaugccaaa acaagugacg aaggacaacg gaguaguacc aaugcaaguc     1260

aacgaaaaag agcuaguagc aauauuagau aaugcaaaag aauaccuccc auuccucuug     1320

aauaaagaua acgacggcaa aacagucuac gauaaaauaa aagcauuguu cgaauaucgc     1380

aucccuuauu acaucggccc cuuaaacacu cacaguacaa aaucauggcu aguuagaagc     1440

aacgaaaaaa ucuauccgug gaauauagag gacguuguag auuucgacaa aagcgcagag     1500

aaauucauug agaacuuaac caguaagugu acauauuuac cucaauacga cguaauacca     1560

aaacauucgc uacuauauaa caaauucugu gugcuaaacg aacucaacaa ccucaaaauc     1620

gacggagaaa aaauaucagu ccaacuaaaa caagauauuu auaacgacuu auucaaaaaa     1680

uacuauaaag uuacuaacaa aaaacucugu gacuacuuaa aaagcagaaa uauagccuuc     1740

gacgaaauca gcggaauaga uaaugacuuu aaaucaaguu uaaaguccua uaaggaccuu     1800

guagcauaca accuuacaga cgaacaaaaa gaagaaauag uaaaagcaau caccauauuc     1860

ggggacgaua aaaaacuuuu gaaaaaacga cucaaaaacg aauuuggagg cugucuauca     1920

gacgacgaaa ucaaaaaaau uugcaaguua aaauauacag gauggucaaa gcuuagcaga     1980

gaauuucuaa cugauguaac ggcagucuac aaagagacug gagaauuaau aaauauaauc     2040

ugcgcccuuu gggaaacaaa uuacaaucug acaucauuac ucuacucaga ugaauuuugu     2100

ugcgacgaua aagaaaacaa aacguuuaau gacaaaauaa acgaauuaaa cucauuugga     2160

aaugacaaaa cgcuaaacca aauaguugag gaacuauacg uaucaccacu cguaaaaaga     2220

ccuauauacc aaagucuacu cauuacuaaa gaacuagaaa aaauuaugaa augcccaccu     2280

aaaaaaauau ucguggaggu ggccagaggc gccgaagaaa aaaaaagaac ugucagcagg     2340

cgcaacaaau uacuagaucu auauaagagc ugcaaaaacg acuauccccu acuauuugau     2400

gaacuacaaa acaauacgac cgacgacgaa cuaaagucag acaaacuaua ucuauauuuc     2460

acacaauuug ggaaauguau guauacagga gaaaacaucg aacuaggaga ucucuucaac     2520

aaaaaccuau acgacauaga ccacauauac ccgcaaucaa aaauaaagga ugacagcuua     2580

gauaaucgcg uccuugugaa gaaaacgaca aacgcaaaaa aggauaacga auauccacua     2640

aguagccaga uaagagaaaa aaugacucca cauuggaaag cacucuuaga caaaggccuu     2700

auuaacaaga aaaaauacga gcgacuugua cgaaauuauc ccuuaagcga cgaagaacuc     2760

ucacaauuua uaucgagaca acucgucgaa acccgccaau caaccaaagc aguagccaac     2820

auacuagaac aguuauaccc caggccagau acagaaaucg ucuacguaaa agccgcccua     2880

gcuuccgaau uccgacauca auaugacaug uuaaaaugca gagaagugaa cgaccugcau     2940

caugcaaaag acgcauaccu aaauaucgua guagguaacg ccuacaacgg aaaauucacc     3000

cauaauaaag caaacuucau aaaaggccuc caaaacaaug acaaagcaua caccguuaaa     3060

cucgacagca ugcuuaaaca uaacauagau ggagccugga uugcagacaa uaaugaaucu     3120

cuaaacauug uaaaaucaac caugaacaaa aacaacauaa gguacacaag auacgcuuuc     3180

gaaaaaaaag guggacuauu uaaaguaaca auacucaaaa aagguaacgg ccaagcauca     3240

aucaaaaaaa acgauaaaag aaauucuauu gaaaaauaug guggauacaa ccaagucucg     3300

agcacauacu ucucaugcgu agaacacggu aaagauaaca accguaugau aucaauguuu     3360

ucgguaaacc uauaugacaa aaacaaguac gaagagaacc cuauagcgua ucuaauagaa     3420

aauuaugguc uaauaaaccc cauaaucaaa guuaaaaaag uaaaaauuca auccugcauc     3480

ucauuugaua auauguuaug ucacuuagga ggaaaagauu cggguggaag ucaaauaucu     3540

uacagaccag gaguacaacu gguacuagga uacgacaaug agagauacau cagaaauguc     3600

auucgaugug cuggagguga ccugcacuug caaacaagua aaaacgaaaa agacauaguu     3660

acagcagaaa aaaacaucga gcuauucaac uuauuaauag aaaaaaugaa aaacaauaua     3720

uauggagucc uuuacagcaa ccuaggagac aaacuauacu guucuaaaga aaaauucgaa     3780

aaacuagaua uaguagaaca augcaaugua auaaaaaaug uacucacaau auuaaaaaac     3840

aaugcaucaa agggugaucu aaaaaguauu gguaucaaag cuaacggugc uauuagauug     3900

aauaacaaca uauccgaaau aaaaaaaguc aaaucgauaa aacuuauaaa ucaaagcgua     3960

acaggacuau uugagcaaga aauugaacuu uuaaaucuau ga                        4002


<210>  5270
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5270
augucccaaa auauaguaga uuacugcaua ggacucgacc uaggaacagg uagcguggga       60

ugggcaguag ucgacaugaa ucaccgacug augaaaagaa auggaaagca uuuaugggga      120

ucgagauuau ucucuaaugc agaaacggca gccaccagga gaaguucgcg aagcauaaga      180

cgaagauaca auaaacgaag agaacgaauc agauuacuaa gagcaauacu acaggauaug      240

guauuagaga aagacccuac cuuuuuuaua agauuagagc acacaagcuu cuuagaugaa      300

gaagauaaag caaaauaccu uggcacugau uauaaagaca auuauaacuu auucauagac      360

gaagacuuca augacuacac guacuaccau aaauacccaa ccauauauca ucuaagaaag      420

gcacucugcg aauccaccga aaaagcagau ccucgacuca uauaucuagc acugcaucac      480

auaguuaaau acagagguaa uuuucucuac gaaggacaaa aauucaacau ggaugcaagu      540

aacauagagg auaaauuauc ugauguauuc acucaauuug cagacuucaa caacauacca      600

uacgaagaug augaaaaaaa gaaucuagaa auacuagaaa ucuuaaaaaa accauuauca      660

aaaaaggcaa aaguagacga aguuauggcc cuaauaucac cagaaaaaga auuuaaaagu      720

gcauauaaag aacuuguaac cggaaucgcc ggcaacaaaa ugaacguuac aaaaaugaua      780

cuaugcgaau caauaaaaca gggugauagc gaaauaaaau uaaaauucuc agauucaaac      840

uacgacgauc aauucaguga aguagaaaac gauuuaggag aauacguaga auuuaucgac      900

aguuuacaca acaucuacuc auggguagaa cuacaaacaa uaaugggagc uacccacacg      960

gauaaugcuu caauaucgga ggcuaugguc ucuagauaca auaaacauca ugaagaccua     1020

caauuauuaa aaaaaugcau caaagauaau guacccaaaa aauacuucga uauguucaga     1080

aacgacagug aaaaaguuaa aggcuauuac aacuacauaa acagacccaa uaaagcacca     1140

gucgacgaau uuuacaaauu uguaaaaaaa ugcauagaaa aaguagacac accggaagcu     1200

aaacaaauuc uccacgauau cgaacuugaa aacuucuugc ucaaacaaaa cagucguacu     1260

aauggaucag ucccauacca aaugcaacua gacgagauga ucaaaauaau agacaaucaa     1320

gcgaaauacu auccugucuu aaaagaaaaa agagaacaac ugcuuucaau auuaacuuuc     1380

cguaucccau auuacuucgg gccauuaaac gaaacuucag aacacgcaug gauuaaaaga     1440

uuagaaggaa aggaaaacca acgcauacua ccauggaacu accaagacac cguugaugua     1500

gaugcuacug cagaaggauu uauaaaacga augagaaguu acuguacuua cuuuccagac     1560

gaggaagucu uacccaaaaa uagucuuauu guuucaaagu acgaaguuua caacgaauua     1620

aacaagauaa gagucgauga uaaacuacuu gaaguggaug uaaaaaacga uauauauaau     1680

gaacucuuca ugaaaaacaa aacuguaacc gaaaaaaaac uaaaaaacug guuaguaaac     1740

aaucaguguu gcagaaaaga ugcggaaauu aaagguuucc aaaaagaaaa ccaauuuuca     1800

acgagucuaa cgccauggau cgauuuuaca aacauauucg guaagauaga ucaaucgaau     1860

uuugacuuaa uagaaaagau cauauaugau uuaacaguau ucgaagacaa aaagauaaug     1920

aaaagacgac uaaagaaaaa auacgcucuc ccagacgaua aaauaaagca aauauuaaaa     1980

cuaaaauaca aagauuggag uagacuaagu aaaaaacuau uggacggcau uguagcagau     2040

aacagauuug gaaguucagu gacaguccua gacguacuag aaaugucacg ccugaacuug     2100

auggaaauca uuaacgacaa ggaacuagga uaugcgcaaa ugauagaaga agccaguucc     2160

ugucccaaag auggaaaauu cacauaugaa gaaguugcca aacuugccgg cucaccagca     2220

cuuaagagag guauauggca aucauuacaa auuguagaag aaauaaccaa aguuaugaaa     2280

ugucgcccaa aguacauaua caucgaauuc gaaagauccg aagaagccaa agaacguaca     2340

gaaucaaaaa ucaaaaaauu agagaacgua uauaaagacc uugaugaaca aacaaaaaaa     2400

gaauauaaau caguacuaga agaauuaaaa ggauuugaua auacaaaaaa gaucucauca     2460

gacagucuau uuuuauauuu uacgcaauua gggaaaugua uguacagugg aaaaaagcua     2520

gauauugaua gucuagauaa auaccaaaua gaccauauag ucccccaaag ccucguaaaa     2580

gacgacagcu uugacaacag aguacuugua cucccuucug aaaaccaaag aaaacuugau     2640

gaucucguag uaccuuucga uauucgcgac aaaauguauc gauuuuggaa gcuacuauuc     2700

gaucacgaac uaauaucacc caaaaaauuc uauucgcuaa uaaaaacaga auauacagaa     2760

agagacgaag aacgauucau uaauaggcaa cuaguugaaa caagacaaau aacaaaaaac     2820

guaacacaaa ucauagaaga ccauuauuca acaacaaagg ucgcagcaau aagagcaaau     2880

cuaucacacg aauucagagu aaaaaaucac auauacaaaa auagggacau aaaugauuau     2940

caucaugcac acgacgccua cauaguggca cuuaucggug gcuucaugag ggaccguuac     3000

ccaaacaugc augauucaaa agcagucuau uccgaauaua ugaaaauguu ccguaaaaau     3060

aaaaacgacc aaaaaaaaug gaaagauggu uucguaauaa auucaaugaa uuauccauac     3120

gaaguagaug gaaaacuaau auggaaccca gaccucauua acgagauaaa gaaauguuuc     3180

uauuacaaag acugcuacug cacgaccaaa cuagaccaga aguccggcca aauguuuaac     3240

uuaacugugu uaccgaacga ugcacacagu gcuaaaggaa ccacagaagc agucaucccu     3300

guuaacaaaa aucgaaaaga cguaaacaaa uacggcggau uuuccggacu ucaauacguu     3360

aucgccgcaa uagaaggcac uaaaaaaaaa ggaaaaaaau uagugaaagu uagaaaacua     3420

agcggaaucc cuuuauaucu aaaacaagcu gauauaaaag aacaaaucga auacguagag     3480

aaagaggaaa aguuaagcga cgucaaaauu auuaaaaaca acauuccacu caaucaauua     3540

aucgaaauag acggacgcca guaccuucuc acauccccaa cagaaugugu gaacgcuaug     3600

caacuagucc uaaaugaaga gcaauguaaa cugauugccg auauuuacaa cgccaucuac     3660

aaacaagacu uugauggccu ugacaacaug uuaaugaucc aacuauaccu acaacuaaua     3720

gauaaauuaa agacccucua cccaaucuau auggggauag uugaaaaauu ugaaaaguua     3780

acagaagacu ucguaucgau aagcaaagaa gaaaaagcga acguaauuaa acaaauguua     3840

auaauaaugc acaaaggacc ccaaaauggc aacauuauau acgacgacuu caacgucgga     3900

aaaagaauag guagacucaa uggcagaaca uucuaccuag acaauauuga auuuauauca     3960

caaucaccaa cagguauaua caccaaaaaa uacaaacugu ga                        4002


<210>  5271
<211>  4002
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5271
auguauaccu auuacuuuaa ggaaagugag augguauaug augugggucu cgauauaggu       60

acaggcagcg ucggaugggu agcucucgac gaaaauggaa aacuagcacg agcaaaagga      120

aagaacuuag uaggcguaag acuauucgac acagcacaaa cugcagcaga uagaagagga      180

uucagaacaa ccagaagaag auuaaguagg cgcaaauggc gacuaaggcu acuagaugaa      240

cuguuuagug ccgaaauaaa ugaaauagac agcagcuuuu uucaacgucu caaauauuca      300

uauguacacc caaaagacga agagaacaaa gcacauuacu auggugggua ucuauuucca      360

accgaagaag aaacaaagaa auuccauaga aguuauccaa ccauauauca cuuaagacaa      420

gaacugaugg cucaaccaaa caaacgcuuu gacaucaggg aaauuuaucu agcaauacac      480

caccucguaa aauaucgcgg acacuuuuua ucuucucaag aaaagauaac aauuggaagu      540

accuauaacc cggaagaccu cgcgaaugcu auugaaguau acgcagacga aaaaggguug      600

ucaugggaau uaaauaaccc ugaacaguua accgaaauua uauccggaga agcaggcuau      660

gguuuaaaca aaucaaugaa agcagaugaa gcuuuaaaac uauuugaauu ugacaauaac      720

caagauaaag uagcaauaaa aacacuccuu gcaggauuaa caggaaauca gaucgauuuc      780

gcaaaauuau uuggaaagga cauaucagac aaagaugaag caaaacuaug gaaacuaaaa      840

cuagaugaug aagcacuaga agaaaaaagu caaacaauac uaucccaacu uacagacgaa      900

gaaauagagc uauuucacgc aguaguacaa gcauacgaug gauuuguacu aauaggacua      960

cuaaacggag cugauagugu uucugcagca auggugcaac uauaugauca acaucgagaa     1020

gaucguaagc uucuaaaauc acucgcacaa aaagcaggac ugaagcacaa acgcuuuucc     1080

gaaaucuacg aacaauuagc ccuugcaaca gacgaagcaa cuauaaaaaa uggcaucuca     1140

acggcgcgag aacuaguuga agaaaguaau cuaaguaaag aagucaagga agauacucua     1200

agaagacucg acgagaauga auucuuacca aaacaaagga caaaagcaaa cagugucaua     1260

ccucaccaau ugcacuuagc cgaguuacaa aaaauacuac aaaaucaagg ccaauauuac     1320

ccuuucuuac ucgacaccuu cgaaaaagaa gaugggcaag acaauaaaau agaggaacua     1380

cucagguuca gaauaccuua uuaugucgga ccacugguua caaagaaaga cguggaacac     1440

gccgggggug acgcagauaa ucauugggua gaaagaaaug aaggcuucga aaagucgagg     1500

guaacacccu ggaacuucga caaagucuuc aauagagaua aggcagccag agacuucauu     1560

gagcggcuaa caggaaacga cacuuauuua auaggggaga aaacacuccc ucaaaauuca     1620

cuaagauacc aguuguuuac aguacuuaac gaauuaaaua acguaagggu uaauggaaaa     1680

aaauuugaca guaagaccaa agcagaucua auaaacgacc ucuucaaagc gaggaaaaca     1740

gucucacuuu cagcauuaaa agacuaccua aaagcacagg gaaaaggaga cgucaccaua     1800

acaggacuag cagacgaauc uaaguucaau agcucacuua gcucguauaa ugaccucaaa     1860

aaaacauucg augcugagua cuuagaaaau gaagacaauc aagaaacucu agagaaaauc     1920

auagaaauac aaacuguauu cgaagacagu aaaauagcuu caagagaauu aaguaagcua     1980

ccacuagacg augaucaagu aaaaaaauua ucucaaacac auuacacagg auggggcaga     2040

cucagcgaaa aauuauuaga cucaaaaaua auagaugaaa gaggacaaaa aguaucaaua     2100

cucgauaaac uaaaauccac gucacaaaac uucaugucca uaaucaauaa cgauaaauac     2160

ggaguacaag cauggaucac agagcaaaau acaggaagcu ccaaacucac auuugacgaa     2220

aaagugaacg agcuaaccac cagcccagcc aauaaaagag gcauuaaaca aucauuugca     2280

guauuaaaug auauaaaaaa agcaaugaaa gaagaaccaa gacgcguaua ucucgaauuc     2340

gcaagagaag aucaaacaag cguaagaucu guaccaagau acaaccaauu aaaagaaaaa     2400

uaccagucga aaagcuuauc agaagaagca aagguucuaa aaaaaacccu agauggaaac     2460

aaaaauaaaa ugagugauga ucguuauuuu cuuuacuuuc aacaacaagg uaaagacaug     2520

uauacuggua gaccaaucaa cuucgaaaga uuaucccaag acuaugacau agaucacaua     2580

auuccacagg cauucacuaa agacgauagu cuugacaaua gaguauuagu uucccgcccu     2640

gaaaacgcca gaaaaaguga uaguuucgca uauaccgaug aaguccaaaa acaggaugga     2700

ucauuaugga cuucauuauu gaaauccgga uucaucaaca gaaaaaaaua cgaaagguua     2760

acaaaagcug gcaaguaccu agauggacaa aaaacggguu ucauagcgag acagcuugug     2820

gaaacgagac aaaucauaaa aaacguugcc ucacuuaucg aaggugaaua cgaaaauuca     2880

aaagcugucg caauaagaag ugaaauaacc gcagacauga gacuacuagu cggcauuaaa     2940

aaacaucgag aaauaaacuc auuccaucau gcauuugaug cgcuucuuau aacagccgca     3000

ggacaauaca ugcaaaaucg auauccugac agggauucua ccaauguuua caaugaauuu     3060

gaccguuaua caaaugauua cuuaaagaau cuaagacaac uaagcagcag agacgaggug     3120

cgucgacuaa aaaguuucgg cuucguugua ggaacaaugc gaaaagguaa ugaggacugg     3180

ucugaagaaa auacaaguua uuuacgaaaa gugaugaugu ucaaaaauau uuuaaccacc     3240

aagaaaacag aaaaagacag ggguccacua aacaaagaaa ccauauuuuc accaaagucg     3300

ggaaaaaaac uuaucccacu uaacuccaag agauccgaua cagcccuaua cggaggguau     3360

ucuaacguau auagcgccua uaugacauua gucagagcaa auggcaaaaa ucuauuaaua     3420

aaaauuccca uaucaauagc aaaucagaua gaaguaggga acuuaaaaau aaaugauuau     3480

auagugaaca auccugcuau uaaaaaauuc gaaaaaauuc uaaucagcaa acucccguug     3540

ggucaacuag uaaacgaaga cggaaauuua auauaccucg cauccaacga auacagacau     3600

aacgcuaaac aacuuuggcu aucuacaacu gacgcagaca aaauagcauc aauauccgaa     3660

aauucaucag acgaagaguu acuagaagca uacgauauuc uaacuucgga aaacguaaaa     3720

aauagguucc ccuuuuuuaa gaaggacauu gacaaacuca gucaaguucg ggacgaauuc     3780

uuagacucag auaaacgcau agcagucaua caaacaaucc uaagagguuu acaaauagac     3840

gcagcauauc aggcccccgu aaaaauaauu ucaaaaaaag ucuccgauug gcauaaacua     3900

caacaaucag gaggaauaaa acucagcgau aauuccgaaa ugauauacca aucggccacu     3960

ggcaucuuug aaacgagggu uaaaauuuca gaccuacucu ga                        4002


<210>  5272
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5272
augaaaaacc cuuauacuau agguuuagau aucgguacua auuccguugg augggcaguu       60

cuaaccaacc aauaugacuu gguaaaaaga aaaaugaaag uagcaggaaa uucagacaag      120

aaacaaauaa agaaaaauuu cuggggagua agacuauucg acgacggcca aacugcaguc      180

gauagacgga ugaaucgcac ugcccgaaga aggaucgaaa gacgcagaaa ucgcauuagu      240

uaucuacaag aaauauucgc agucgagaug gcgaacaucg acgcaaauuu cuuuugccga      300

uuaaaugauu cauucuacgu agacucugag aaaagaaauu cacgucaucc cuucuucgcg      360

acaauagaag aagaaguagc cuaccacaaa aacuauagaa caauauacca uuuacgagaa      420

gaauuaguaa auucuucaga aaaagcggau cuacgccuag uauaccuagc acuugcacau      480

auaaucaaau auagaggaaa cuuucuaauu gaaggagcau uggacacuaa aaacaccagu      540

gucgacggag uguacgaaca auucauacaa accuacaacc aaguauucau gucaaauauu      600

gaagaaggua ccuuagcuaa aguagaagaa aauauugagg uagcuaacau auuagcagga      660

aaauucacca gaagggaaaa auuugaaaga auccuucaac uuuauccagg ugaaaaauca      720

accggaaugu ucgcgcaauu caucagucuu auaguaggcu caaaaggaaa uuuccaaaaa      780

guuuucgacc uaauagagaa aacggauaua gaaugugcga aagauucaua cgaagaagac      840

cucgaggcuu uacuugcgau uauuggcgau gaauacgcag aacuauuugu agcagcgaaa      900

aauacauaca acgcaguugu acuaaguuca aucauaaccg ucaccgcaac agaaacaaau      960

gccaaacuuu cagccuccau gauugaacga uucgaugccc acgaaaaaga ccuaggugaa     1020

uugaaagcau ucauuaaauu acacuuacca aaacaauacc aagaaaucuu uaauaaugca     1080

gccauagaug gauaugcggg uuacauagau ggcaaaacaa aacaaguaga cuucuacaaa     1140

uaucuaaaaa ccauccuuga gaacauagaa ggagccgauu acuucauagc aaaaauagaa     1200

gaggaaaauu uucugcgaaa acaaagaacc uucgauaacg gcgcuauacc ucaccaauug     1260

caccuugaag aacuagaagc caucauacac caacaagcua aauacuaucc auuucuaagg     1320

gaagacuaug agaaaauaaa gagcuuaguu acauucagga uacccuacuu uguaggaccg     1380

cuagccaaag gucaaucuga auuugcaugg cugacaagga aagccgaugg agaaaucaga     1440

ccauggaaca uagaagaaaa aguagacuuc ggaaaaucag cgguagacuu cauagagaaa     1500

augacuaaca aagacacaua ucuuccaaaa gaaaauguac ucccaaaaca cucuuuaugc     1560

uaucaaaaau auauggucua uaacgaauua acaaaaguaa gauauauaga cgaucaaggu     1620

aaaacaaauu acuucuccgg ccaagaaaaa caacaaauuu ucaacgacuu auuuaaacag     1680

aaaagaaaag ucaaaaagaa agaucuugaa cuauuccuua gaaauaucaa ucacauugaa     1740

ucaccaacca uugaaggcuu ggaggacuca uucaacgcau cauaugcaac uuaccacgac     1800

cuacuuaaag ucggaaugaa acaagaaauu cuagacaacc cucuaaacac agaaaugcua     1860

gaagacaucg uaaaaauccu aacuguuuuu gaagacaagc cgaugaucaa ggaacaacua     1920

caacaauuuu cggauguacu agauggugga guacuuaaaa agcuagagcg acgccauuau     1980

acaggauggg guagguuguc cgcaaaacua cuuguaggaa uaagagaaaa acaaagccau     2040

cuaacaauau uagauuacuu aaugaaugac gacgggcuaa acagaaaucu gaugcaacua     2100

auaaaugaca gcaauuuauc auucaaauca auuauagaaa aagaacaagu cagcacaaca     2160

gacaaagacc uucaaucuau uguugcagau cuagcaggca gcccggccau caagaaaggu     2220

auccuucagu cuuuaaaaau aguagacgaa uugguaucga uaaugggaua cccaccacaa     2280

acuaucguag uugaaauggc cagagaaaau cagaccacug guaaaggcaa aaauaauuca     2340

aaaccccgau auaaaucauu ggaaaaagcc auuaaagaau uuggaucaaa aauauuaaaa     2400

gaacacccaa cagacaacca agaacucaaa aauaauaggc uauaccucua cuacuuacaa     2460

aauggcaaag acauguauac aggacaagaa uuggacauac auaacuuaag uaacuacgau     2520

auagaccaca uuguuccaca aucauuuaua acugacaacu caauugauaa ucugguucuu     2580

acaagcucug caggaaaccg ugagaaagga ggagauguac caccacucga gauaguuaga     2640

aaacgaaaag ucuucuggga aaaauuauau caaggaaauc uaauguccaa aaggaaauuc     2700

gacuauuuaa cuaaagccga acgugguggu cucacggacg cagacaaagc aagauucauc     2760

cacagacagc uaguagaaac ccgccaaauc acuaaaaacg uagcaaauau acuacaucaa     2820

agauucaaca augaaacaga caaccauggc aauacaaugg aacaaguaag aauagucaca     2880

uuaaaauccg cuuuaguaag ucaauuuaga aaacaauuuc aauuauauaa aguaagagaa     2940

guaaaugacu accaucaugc acacgaugca uaccuaaaug gagugguggc uaauacacuu     3000

uuaaaagucu acccacaacu agaaccagag uucgucuaug gcgaauacca ucaauucgac     3060

ugguucaaag caaacaaagc aacagccaag aaacaauucu auacaaauau aaugcuauuc     3120

uuuggacaaa aagaacgaau aaucgacgaa aauggagaaa uauuauggga uaagaaauau     3180

uuagaaacga uuaagaaagu acuagauuau cgacaaauga acauuguaaa gaaaaccgaa     3240

auccaaaaag gagaauuuuc uaaagcaaca auaaaaccaa aagguaacag caguaaacuu     3300

auacccagaa aagaaaauug ggauccaaug aaauauggcg gucuagacuc accuaacaug     3360

gcuuacgccg uaauaauaga acaugccaaa gggaaaaaga aacuaauuuu ugagaagaaa     3420

auaauuagaa ucaccaucau ggaaagaaaa auguuugaaa aagacgaaga agcauuuuua     3480

gaagaaaaag gauaucgaca uccuaaagua cuaacuaaau ugccaaaaua uaccuuauac     3540

gaaugcgaaa aaggacgccg aagaaugcua gcaagcgcca augaagcaca gaaaggcaac     3600

caacuggucc uaucaaauca uuuaguaagu cuccucuauc augcuaaaaa uugugaagcc     3660

ucagacggaa aaucucuaaa auacauagag gcacauagag agacauuuag cgaguuauua     3720

gcccaaguau cagaauuugc caccagauau acuuuagccg acgccaaccu gagcaaaaua     3780

aauaaccuuu uugagcaaaa uaaagaaggu gauauacaag caaucgccca gagcuucgua     3840

gaucuaaugg cauucaacgc uaugggagca cccgccucuu ucaaauucuu ugaagccacc     3900

auagacagaa aaagauacac aaauuuaaaa gaauuacuuu caucaacgau cauauaucaa     3960

ucaauaacag guuuauacga aucaagaaaa agauuagacg auuga                     4005


<210>  5273
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5273
augaaaaaac cauacaccau aggucuagau auaggaacaa acucuguugg augggcugcu       60

cuaacugacc aauaugaucu aguaaaaaga aagaugaaag uugccgguaa cucagaaaaa      120

aagcaaauua aaaagaacuu auggggcguc aggcugguag acgaaggaaa aacugcagca      180

caucgacgcg uuaauagaac aacaagaaga agaauugaaa gacgacgaaa cagaauuuca      240

uaucuacaag aaauuuuuac ggcagaaaug uucgagguag augcaaacuu uuuuuacaga      300

cucgaagauu cguuuuacau agagucagaa aaacgacaaa guagacaccc auuuuucgcu      360

acaauagaag aagaagucgc auaucacgaa aauuacagaa caaucuacca uuuaagagaa      420

aaacuaguaa auucaucgga caaggcagac cuaagauugg uauacuuagc ucucgcacau      480

aucaucaaau acagggggaa uuuccuaauc gaagguaaac uagacacaaa aaauacuagc      540

guagacgagg uuuuuaagca auucauaaaa acauauaacc aaguauuugc cagcgauauu      600

gaagaaggaa guuuaacaag gauagaggaa aacaaugagg uagcaaaaau cuuuucugaa      660

aaacucacca aaagggaaaa acucgauaaa auuuugaacu uauaucccaa ugaaaaaagc      720

acugacuuau uugcccaauu uauaagccua aucauaggau cuaagggcaa uuucaaaaaa      780

uuuuucaacc ucacagaaaa aaccgacauu gaaugugcga aagauaguua cgaagaagac      840

uuagaaguuu uacuagcccg agugggagac gaauacgcag aaauauuugu ggcagcgaaa      900

aaugcguaca augcuguagu auuaucuagu aucauuacag uauccaacac cgaaaccaaa      960

gcaaaacuau cagcuucuau gauagagaga uucgauaaac augacaaaga ccuuaaacgu     1020

augaaggcau ucuuuaaagu cagacuacca gaaaauuuca augaaguauu uaaugaugua     1080

gaaaaagaug gcuaugcggg guacauagaa ggcaaaacaa aacaagaagc auucuacaaa     1140

uacaugaaaa aaaugcuuga gcacgucgaa ggggcggauu acuuuaucaa ccaaaucgaa     1200

gaagaaaauu uuuuacggaa acaacgaaca uucgauaacg gagcaauccc acaucaacua     1260

caucucgagg aacuugaagc uauacuacac caacaagcga aauauuaccc cuucuuaaaa     1320

gucgacuacg aaaaaaucaa gagccuaguc acuuuuagaa uaccuuacuu cguaggaccc     1380

uuagccaaug gacagaguga auucucgugg uuaacacgca aagcagacgg agaaauaaga     1440

ccuuggaaca uugaagaaaa agucgauuuc ggaaaaagug caaucgacuu uauugaaaaa     1500

augacaaaca aagauaccua ccucccaaaa gaaaacguau uaccaaaaca cagcaugugc     1560

uaucagaaau acaugguaua uaaugaauua acaaaaauaa gauacacaga ugaccaaggc     1620

aaaacucacu acuuuagcgg gcaagaaaaa cagcaaauau ucaaugacuu auucaaacaa     1680

aaaaggaaag uuaaaaaaaa ggacuuagaa uuauuccucu auaacaugaa ucauguagaa     1740

ucaccaacag uagaaggagu agaggaugca uucaauuccu cuuuuaccac auaccacgac     1800

cuacagaaag uaggaguccc ucaagaaaua uuagaugauc cacucaacac ugaaauguua     1860

gaagaaauaa uuaaaauacu aaccguauuc gaagacaaaa gaaugaucaa ugaaagauua     1920

caagaauuca gcaaugucuu agacgaagcg guacuuaaaa aacuagagag aagacacuau     1980

acuggcuggg gaagacuaag ugcuaaauua cuaaucggaa uacgcgacaa agaaucccac     2040

cugacuauac uugauuaucu aaugaacgac gauaaacaca acaggaaccu uaugcagcuu     2100

auaaacgaca guaaccuuuc cuuuaaguca aucauagaaa aagaacaagu cucaacagca     2160

gauaaagaua uccagucaau agucgcggac uuagcaggga gcccagcaau aaaaaaagga     2220

auccuccaaa gccucaaaau agucgacgaa uuaguaggaa ucauggguua uccgccacaa     2280

acgaucguug uagaaauggc aagggaaaau caaaccaccg gaaaagggaa aaacaauuca     2340

aaaccaagau uuaccucauu agaaaaagcu auaaaagaac uaggaagcca aauucucaaa     2400

gaacacccaa cagauaacca aggauuaaaa aaugacagac uauaccuuua cuaucuacaa     2460

aauggaaaag acauguauac aggucaagaa uuagauauac acaaucuaag uaauuaugau     2520

auugaucacg uaguaccuca aagcuuuaua acagauaacu ccaucgacaa uagaguacua     2580

gcuucauccg cugcaaauag ggaaaaagga gacaacguuc ccucgcuaga gguaguaaga     2640

aaaagaaaag ucuauuggga aaaacuguau caagcaaaac ucaugagcaa aagaaaauuu     2700

gauuaucuaa cgaaagcaga acgaggaggc cugacugaag cagauaaagc aagauucauu     2760

cauagacaac uagucgaaac acgucaaauc accaaaaaug uugcaaacau uuuacaucaa     2820

agauuuaauu gcaaaaaaga ugaguccgga aacgucaucg aacaaguuag aaucguaaca     2880

cuuaaagcag cauuaguauc gcaauucaga aagcaauucc aauuauacaa aguacgagaa     2940

gugaacgacu accaucacgc acacgacgcc uaccuuaacu guguugucgc uaauacauua     3000

cuaaaaguuu acccacaacu cgaacccgaa uucguguaug gagauuauca ccaguucgau     3060

ugguucaaag ccaacaaagc cacagcuaaa aaacaauuuu auaccaauau aaugcuauuu     3120

uucgcaaaaa aagaucguau aauagaugaa aacggcgaaa uccucuggga caaaaaauac     3180

cucgacacaa uaaaaaaggu auuaaauuau aggcaaauga acauaguaaa aaagacagaa     3240

auacaaaaag gugaauucuc aaaugccaca gcaaacccaa agggaaauag uagcaagcuu     3300

auccccagaa aagcugauug ggauccaauu aaauauggcg guuucgacgg uucaaauaug     3360

gcuuacgcca ucguuauuga acaugaaaaa agaaaaaaaa aaacgguaau aaaaaaagaa     3420

cuaauccaga uaaauauaau ggagaggaca gcguucgaaa aggaucaaaa agaauuccuc     3480

gaagguaaag gauaccguaa uccaaaaguu aucacaaaga uaccuaaaua caccuuauac     3540

gaaugugaaa acggaagaag aagaaugcua ggaucagcca augaagcaca aaaaggaaac     3600

caaauggucc ugccgaacca ccuuaugacc uuacuauauc acgcgaaaaa cugugaagca     3660

ucggacggua aauccuuagc auauauagag ucucaucgag aaauguuugc agaauuauua     3720

gacucaauau cugaauucgc gucucguuac acucuugcug augcaaaucu agagaaaaua     3780

aacacaaucu ucgaacaaaa caaaagugga gacgucaaag ucauagcaca aagcuuuguu     3840

aaucuacucg aauuuaaugc gaugggagcc ccagcaucgu ucaaauauuu cgagaccaac     3900

aucgaacgua aaagguacaa caauuuaaag gaacuauuaa augccacaau aaucuaccaa     3960

ucgaucacag gacuauauga ggcaagaaaa aggcuugaug auuga                     4005


<210>  5274
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5274
augaaaaacc cauacacaau agguuuagau auaggaacaa acagcguagg augggcagua       60

uuaaccgacc aauacgaucu uguaaaacgu aaaaugaaag uagcuggaaa cagcgauaag      120

aaacaaauca agaaaaacuu cuggggaguc aggcuauucg augauggaca aacagcagua      180

gacaggcgga ugaauaggac ugcucgacga cgcauagaaa gacguaggaa cagaauaagu      240

uaucuacaag aaauauuugc agucgaaaug gcaaauauag acgcaaauuu cuucugucgc      300

cucaacgacu cauuuuaugu agauagugag aaaagaaaua guagacaccc cuuuuucgcc      360

acaaucgaag aagaagucgc auaucauaaa aacuacagaa caauuuacca ucuacgagaa      420

gaguuaguga acagcaguga aaaagcagau uuaagguuag uguauuuagc acuagcacac      480

auuauuaaau acagaggaaa uuuucucauc gaaggagcac uagauacgaa aaauacuuca      540

guagacgaag uauacaaaca auucauucaa acauacaauc aaguauucau gaguaacaua      600

gaagaaggag cucuggcaaa aguagaagaa aacacggaag ucgcaaguau auuggcagga      660

aaauucacua gaagggaaaa auuugaaaga auacucagac ucuacccugg cgaaaaauca      720

accgguaugu ucgcgcaauu uauuucccuc auaguaggga acaaaggaaa cuuccaaaaa      780

guuuucaacc ugguagagaa aacagacaua gaaugugcaa aagauucaua cgaagaagac      840

uuagaagcac uauuagcaau aauaggugac gaauaugcgg aauuguucgu agcugccaaa      900

aacacauaca acgcagucgu auuaucauca auaauaacag uaacagcaac ugaaacaaac      960

gcgaaacuau ccgccuccau gauagaacga uuugacgcac augagaaaga acuaggagaa     1020

uuaaaagccu uuauuaaauu acaucugcca aaacaauauc aagaaauauu uaauaaugca     1080

gaaauagaug gauacgcagg uuauaucgac ggaaaaacaa aacaaguuga uuuuuacaaa     1140

uaucuaaaaa ccacacuaga aaauguagaa ggagcugauu acuucaucac uaaaauugaa     1200

gaagaaaauu ucuuaaggaa acaaagaaca uuugacaacg gcguuauacc ccaccaacua     1260

cacuuagaag aauuagaagc aaucuuacac caacaagcua aauacuaccc auuuuuacgu     1320

gaaggcuaug acaaaaucaa aucacuggua acauuuagaa uaccguacuu uguaggacca     1380

uuagcuaacg gacaaucaga guucgcuugg cucacaagaa aagaugaugg agaaauaagg     1440

ccuuggaaca uagaagaaaa aguugauuuc ggaaaaucag caguagacuu caucgagaaa     1500

augaccaaua aagacacaua ccuaccgaaa gaaaauguuc uaccaaaaca cucauuaugc     1560

uaccaaaaau acaugguuua uaaugaacua acaaaaaucc gauacauuga cgaccagggu     1620

aaaacuaacu acuucagcgg acgugaaaaa caacaaauau uuaacgaucu uuuuaaacag     1680

aaaaggaaag ucaagaagaa agacuuagaa cuauucuugc gaaauauaaa ccauauagaa     1740

ucgcccacua uagaaggcuu agaagacagu uucaaugcaa gcuacgccac uuaucaugac     1800

uuacuuaaag uaggacuaaa acaagaaaua cuagacaacc cuuuaaacac cgaaaucuua     1860

gaagacauug uaaaaauacu aacuguauuc gaagauaaac gaaugaucaa agaacaguua     1920

gaacaauucu cagauguacu agauggaguc gucuugaaga aacuagaacg ccgccauuac     1980

acggguuggg gaagauuauc cgcaaaauug uugguuggga uucgugacaa acaaucgcac     2040

cucacuauac uagauuauuu aaugaaugac gacggauuaa accgaaacuu aaugcaauua     2100

auaaacgacu caaaccucag cuucaaauca auaauugaaa aagaacaagu aucaacaacu     2160

gauaaagauc ugcaaagcau aguagcugac uuagcaggau cgccugcuau caagaaaggc     2220

auucuacaau cacucaaaau uguugaugag cuagucagua ucaugggaua uccaccccaa     2280

acaauagugg uugaaauggc gagagaaaau caaacaacug gaaaaggcaa aaacaauagc     2340

aaaccaaggu auaaaagccu agaaaaagca auaaaagauu uuggaucgca aauuuuaaaa     2400

gaacacccca ccgauaacca agaacucaaa aacaauagac uauauuuaua uuacuuacaa     2460

aauggaaaag acauauacac aggacaagaa uuggacauac acaaucucuc caauuaugac     2520

auugaucaca uaguaccaca gagcuucaua acugauaauu ccauagauaa ucucguucua     2580

acaucaucag cuggaaacag agaaaaagga ggagacguac caccauuaga aauaguuagg     2640

aaaagaaaag uauuuuggga aaaauuguau caaggcaauu uaaugaguaa acgaaaauuu     2700

gacuaucuaa cgaaagccga aagagggggc cuaacugaag ccgacaaagc aagauuuauu     2760

caucgucagc uagucgaaac aaggcagauu accaaaaaug uugcaaauau auuacaccaa     2820

cgauuuaaua aagaaacgga caaucacggg aauacaaugg aacagguaag gauaguaacu     2880

uuaaaaagcg cccuaguauc acaauuucga aaacaguuuc aacuuuacaa agucagagaa     2940

gugaacgacu aucaccacgc acaugacgcu uaucugaaug gcgugguagc gaauacacua     3000

cuuaaaguau acccacaauu agaaccggaa uuuguuuaug gcgaauacca ccaauuugac     3060

ugguucaaag caaauaaagc aacugccaag aaacaauucu acacaaauau uauguuauuc     3120

uuugcacaaa aagaaaggau cauagacgaa aacggcgaaa uuuuauggga caagaaauau     3180

cuagaaacca ucaaaaaggu acuagacuac agacaaauca acauaguaaa gaaaacagaa     3240

auucaaaaag gcgaauuuag uaaagcuacc auaaaaccaa aaggaaacag uucgaaacuc     3300

auaccacgca aagaaaauug ggacccaguu aaauacggag gccuagauag cccaaauaug     3360

gcauaugccg uaauaaucga acacgcuaaa gguaaaaaga aaauaguaau cgagaagaaa     3420

cuaauucaaa ucaauaucau ggaacgaaaa auguucgaaa aagacgaaga ggcuuuccua     3480

gaagaaaaag guuaccacca accuaaagua uuaacaaaau uaccaaaaua cacacuauau     3540

gaaugcgaaa aaggcagaag aagaaugcua ucaucagcga augaagccca gaaaggaaau     3600

caauuaguuu uaagcaauca uuuaguuagc cuacuauauc acgccaaaaa cugcgaagca     3660

agugauggaa aaagucuaaa auauauugaa gcacaucgag aaacauucag ugaacuuuua     3720

gcacaaguau cagaauuugc aacaaaauau acucucgccg acgccaauuu gucuaaaaua     3780

aacaaccucu ucgaacaaaa uaaagaagga gacauaaaag cgauugcgca aucauuugua     3840

gaccucaugg cauuuaacgc aaugggagca ccagcaaguu uuaaguucuu ugaagcaaca     3900

auagacagaa aaagguauac aaaucuaaaa gaacuauuaa gcaguacaau uauauaccaa     3960

aguaucaccg gucucuacga aaguagaaaa aggcuugacg acuga                     4005


<210>  5275
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5275
augggucgua aaccauacau acuaucauua gauaucggua caggaucggu aggauacgcu       60

ugcauggaca aaggauuuaa uguacuaaaa uaucaugaca aagacgcacu agguguauau      120

cucuucgaug gugcauuaac ugcacaagaa cgacgacaau uccgaacaag ccgucgaagg      180

aaaaaccgac gcauaaaacg acuaggcuua cuacaagaac ucuuagcucc cuuaguacaa      240

aacccaaacu ucuaccaauu ccaacggcaa uuugccugga aaaacgacaa uauggacuuc      300

aaaaacaaau ccuuaucuga aguccucuca uuuuuaggcu acgaaagcaa gaaauaccca      360

accauauacc accuacaaga agcucuacua cuaaaagacg aaaaauuuga cccagaacuu      420

auauauaugg ccuuauauca ucuaguuaaa uaccgagggc acuucuuauu cgaccacuua      480

aaaaucgaaa aucuaaccaa uaacgacaau augcacgacu ucguagagcu aaucgagacc      540

uacgaaaacu uaaauaauau caaacuaaac uuagacuacg agaaaacuaa aguaaucuau      600

gaaauacuca aagauaacga aaugaccaaa aacgaccgag caaaacgcgu uaaaaauaug      660

cagaaaaagc uagaacaauu uucaauaaug uuacuuggac uaaaauucaa cgaaggcaaa      720

uuauuuaauc acgccgacaa cgcagaagaa uuaaaaggag caaaucaauc ccacacauuu      780

gccgacaauu acgaagaaaa ucugacacca uuccuuacgg ucgaacaguc ugaguucaua      840

gaacgagcaa acaaaauuua ucuaucauua acacuacaag acauacugaa aggaaagaaa      900

ucaauggcua uguccaaagu ugcugcauau gacaaauuuc guaacgaacu caaacaaguu      960

aaagauauag uuuacaaagc ugauuccaca cgaacccaau ucaagaaaau auuuguauca     1020

agcaaaaaga gccucaaaca auacgacgcg acacccaaug accaaacuuu cagcucacua     1080

ugccuauucg accaauaucu aaucagaccc aagaaacaau acucauuacu caucaaagaa     1140

cucaagaaaa uaauuccaca agauucagaa cucuacuuug aagcagaaaa ugauacacuc     1200

cuuaaaguuu uaaacacaac cgauaaugca uccauaccaa ugcaaauuaa ccuauacgaa     1260

gcagaaacaa uacuucgcaa ucaacaaaaa uaucacgcag aaauaacaga ugaaaugaua     1320

gaaaaaguac ugucacuaau ucaauuccga auaccauacu auguaggccc acuuguaaau     1380

gaucacacag caaguaaauu cggcuggaug gagaggaaau cuaacgaauc caucaaacca     1440

uggaauuuug acgaaguagu agaucgcuca aaaucugcaa cacaauucau aagaagaaug     1500

accaacaaau gcucauaccu aaucaaugaa gacguacuac ccaaaaacuc acuacuauac     1560

caggaaaugg aaguccuaaa ugaacuaaau gccacccaaa uacgauuaca aaccgacccc     1620

aaaaacagaa aauauaggau gaugccacaa aucaaauuau uugccguaga gcauauuuuu     1680

aagaaauaua aaacagucuc acacuccaaa uucuuagaaa ucaugcuaaa cucuaaucau     1740

cgugagaauu uuaugaacca uggugaaaaa uuaucaauuu ucggaacaca agaugacaag     1800

aaauucgccu ccaaacucag uucuuaccaa gacaugacca aaauauuugg cgauaucgaa     1860

ggaaaacgag cacaaaucga agaaauaauu caauggauua ccauuuucga agacaagaaa     1920

auauuaguac aaaaacuaaa agaaugcuac ccugaacuaa cgucuaaaca aaucaaccaa     1980

uuaaagaaau uaaauuacuc aggcugggga cgucucucag agaaacuacu uacacacgca     2040

uaccaaggac acuccauaau agaacuccua agacacucgg acgaaaauuu cauggaaaua     2100

cuaacgaaug auguguacgg auuucaaaau uucauuaaag aagaaaacca aguucaauca     2160

aacaaaaucc aacaccaaga caucgcaaau cuaacuacau cacccgcgcu aaagaaagga     2220

aucuggucaa caaucaaacu gguacgggag cuaacaucaa uuuuugguga gccagagaaa     2280

aucauaaugg aauuugccac ugaagaucaa caaaaaggga aagagcaaaa aucgagaaaa     2340

cagcuauggg acgacaacau caagaaaaac aaauuaaaau ccgucgacga auacaaauac     2400

auaauagaug uagcaaauaa acuuaacaac gaacaacuac agcaagaaaa auuauggcuu     2460

uaucuuaguc aaaauggcaa auguauguau ucaggacagu caauagaucu cgaugcacua     2520

cuaucuccga augcuacaaa acacuacgaa guggaccaua uauucccacg aucauucaua     2580

aaagacgaua guauagacaa uaaaguacuc gucaucaaga aaaugaauca aacaaaagga     2640

gaccaaguac cucuccaguu cauccaacaa ccuuaugaaa gaauugccua cuggaaaucu     2700

cucaacaaag caggauugau uagcgacuca aaacuacaca aacuaaugaa acccgaauuu     2760

acagcaaugg auaaagaagg uuucauacaa cgccaauuag uagaaacacg acaaaucucc     2820

guucacguaa gagacuuccu caaagaagaa uauccaaaca cuaaaguaau acccaugaaa     2880

gcaaaaaugg uuucagaguu cagaaagaaa uucgacauac ccaaaauaag acaaaugaac     2940

gacgcacacc acgccauuga cgcauaccuu aacggaguag uuuaccacgg agcacagcua     3000

gcauauccca auguugaccu guucgacuuc aauuuuaaau gggagaaggu aagggagaaa     3060

uggaaagcac uaggagaguu caacacaaaa cagaaaagua gagaacucuu cuuuuucaag     3120

aaacuagaga aaauggaagu aucacaagga gaacgccuga ucucaaaaau aaaacuagau     3180

augaaucacu uuaaaaucaa uuauuccaga aaacuagcca acauaccaca acaauuuuac     3240

aaucaaaccg cagugucacc caaaacagca gagcuaaaau acgaaucgaa caaauccaac     3300

gaaguaguau acaaaggccu cacuccauac caaacauaug uuguagcaau aaaaagcguc     3360

aacaagaaag gaaaagagaa aauggaauac cagaugauag aucauuaugu auucgauuuu     3420

uacaaauucc aaaacggaaa ugaaaaggaa cucgcucuau auuuggcaca aagagaaaau     3480

aaagaugaag uacucgaugc ccaaauugua uacucauuaa auaaaggaga ccucuuauac     3540

auuaauaacc aucccuguua uuucguaucc agaaaagaag ucauaaaugc aaagcaguuc     3600

gaguuaaccg uugaacaaca acuaucccua uacaacguca ugaauaauaa agaaacuaac     3660

gucgaaaaac uccugauaga auaugacuuc auugcugaaa aagucauuaa ugaauaccac     3720

cacuauuuaa acucaaaacu aaaagagaaa agaguacgua cauucuuuag cgaaaguaau     3780

caaacacacg aagauuucau caaagcacuu gacgaauuau ucaaaguagu uaccgcauca     3840

gccacaagau cggauaaaau uggaucaagg aaaaauucaa ugacccacag agcauuucua     3900

ggaaaaggaa aagauguaaa aaucgccuac acuucgauau caggauuaaa aacaacaaaa     3960

ccuaaaagcc ucuuuaaauu agcagaauca cguaaugagc uauga                     4005


<210>  5276
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5276
augaaaaacc ccuacacaau aggacuggau auaggaacaa auucaguagg cugggcugua       60

cuaacagacc aguaugaucu agucaagcgc aaaaugaaag uagcaggaaa cagcgacaag      120

aaacaaauaa agaaaaauuu cugggguguc agauuauuug acgaagggga aacugcagcu      180

gauagaagaa ugaauagaac ugccagaaga agaauagaaa gaagaagaaa ucgcaucagu      240

uauuuacaag agauauuugc auuagaaaug gcaaacaucg acgcaaauuu cuuuugucgc      300

cugaaugaua gcuuuuaugu ugacagcgag aaaagaaacu ccagacaucc auuuuucgca      360

acaauagaag aagaagucgc auaucacaaa aauuauagaa ccauauauca cuuacgggaa      420

gaacugguaa auaguagcga aaaagcagac uuaagauuag uauaucuugc guuagcacac      480

aucaucaaau acagaggcaa cuuccuaaua gaaggagcau uggauacuaa aaacacaagu      540

guagauggag uauacaaaca auucauacaa acauauaacc aagucuuuau auccaacauu      600

gaagagggca cauuagcaaa gauggaagaa aacacaacag ucgcagauau acuagcagga      660

aaauucacac ggaaagaaaa acuugagaga auacuacagc uauauccugg agaaaaauca      720

acagggaugu uugcacaauu cauaucucuu aucguaggau cgaagggcaa cuuucaaaaa      780

guauucgauc uuguugagaa aacugacauu gaaugugcaa aagacaguua cgaagaagac      840

cucgaagcac uacuggcaau aauuggagac gaguacgcag aauuauuugu ugcugcgaag      900

aauacuuaca augcuguagu ccuuuccagc aucaucacag uaacagacac agaaaccaac      960

gcaaaauugu cagcaagcau gauagaacga uuugacgcuc acgaaaaaga ccuaagagaa     1020

cuuaaagcgu ucauaaaacu acaccuaccu aagcaauaug aagagauauu caguaauguc     1080

gcaauugaug gcuacgcugg cuauauagau gguaaaacaa aacaaguaga cuuuuacaaa     1140

uaccucaaaa cuuuacugga aaacauagaa ggagccgauu acuucauagc uaaaaucgag     1200

gaagagaauu uccuaagaaa acaaagaacc uuugacaacg gugcuauucc acaucaauua     1260

caucucgagg aauuagaagc gauuuuacau caacaagcua aguacuaucc auucuuuaaa     1320

gaagcauacg acaaaauaaa aaguuuaguu acauuuagga ucccauacuu uguuggucca     1380

cuagccaaug gacaauccga uuucgccugg cuaacaagaa aagcugaugg cgaaauaaga     1440

ccuuggaaua uugaagaaaa gguagacuuc ggaaaaucag ccguagauuu uaucgagaaa     1500

augaccaaua aagacacaua ucuaccaaaa gaaaacguuc ucccaaaaca cuccuuauac     1560

uaucaaaaau auaugguuua caaugaacuu acaaaaguac gcuacauuga cgaucaaggc     1620

aaaacaaacu acuucagcgg acaagaaaaa cagcaaaucu uuaacgauua cuucaaaaag     1680

aaaagaaaag uuagcaaaaa ggauuuagaa caauuccucc guaacauguc ccauauagaa     1740

agcccaacua uugaaggucu agaagauagu uuuaauucuu cuuacgccac auaucaugac     1800

uuguuaaaag uaggcauaaa acaagaagua cuagaaaauc cacuaaacac agaaauguua     1860

gaagauauug uaaaaaucuu aacaguguuu gaagacaaaa gaaugaucaa ggaacaauua     1920

caacaguuca gugacguauu agauggagca guacucaaga aacuugagcg acgccacuau     1980

acaggcuggg gccgacuauc agcaaaacuu cuaguaggga uaagagacaa acaaucacac     2040

cuaacaauau uggauuacuu gaugaaugau gauggauuga acagaaaccu caugcaacua     2100

auaaacgaua guaaucuauc cuucaaaagc auaaucgaaa aagagcaagu aaguacaaca     2160

gauaaagacu uacaaagcau aguagccgau cuagcgggau caccagcuau aaagaaaggc     2220

auccuacaaa gccuaaaaau aguagaugaa uuaguaucaa uaaugggaua uccaccgcaa     2280

acaauuguag uggaaauggc uagagaaaac caaaccacag uaaaaggcaa aaauaacagc     2340

cgaccuagau auaagagcuu agagaaagca auaaaagagu ucggcucuca gauacuaaaa     2400

gaacacccua cagauaauca agaacuaaga aauaauaggu uguaccuaua uuauuuacaa     2460

aauggaaaag auauguauac gggacaagaa uuggacaucc auaaucugag uaacuaugac     2520

auagaccaua uuguucccca aaguuucauu acagacaaca guauagacaa ucuagucuua     2580

acaucuucug cuggaaauag agaaaaagga gaugacguac cucccuuaga aauagucagg     2640

aaacgaaaag ucuucuggga aaaacuauuc caaggcaacu uaaugucaaa acggaaauuc     2700

gauuacuuga cuaaagcaga acgaggagga cuaacugaag cagauaaagc cacauucauu     2760

cauagacaau uaguggaaac aagacaaaua acuaaaaaug uugcuaacau cuuacaucaa     2820

agauuuaaca augaaacaga caaucacggc aacaacaugg aacaaguacg gauaguaaug     2880

cuaaaaucag cccuggucuc ccaauucagg aaacaauuuc aacucuauaa aguaagggag     2940

guuaacgacu accaccacgc ccacgaugcc uacuugaaug gagugguagc uaauaccuua     3000

cucaaaguau acccacaauu agagcccgag uucgucuaug gugaauacca ccaauucgac     3060

ugguucaaag caaauaaagc aacagcaaag aaacaauuuu acacaaacau aaugcuauuc     3120

uucgcgcaaa aagaaagaau cauagaugaa aauggugaaa uacuuuggga uaagaaauac     3180

cuagaaacaa uuaagaaagu auuagauuac cgacaaauga acauagucaa gaaaaccgaa     3240

auacaaaaag gagaguucuc aaaagcaacu auaaaaccua aagguaauuc aucaaaauua     3300

auaccaagaa aagaaaauug ggacccaaug aaauauggag gacuagacuc acccaacaug     3360

gccuacgccg ucauaauuga acacgcaaaa gguaaaaaga aaguaguguu ugaaaagaaa     3420

auaauaagaa uaacaauaau ggaacgcaaa gcauucgaaa aagaugagaa aaguuuucua     3480

gaaaaacaag gauauagaca accuaaaguu cuaaccaaau uaccgaaaua cacuuuguau     3540

gaaugugaaa augggagaag aagaaugcua gcaucugcca augaagcaca aaaaggaaac     3600

cagcaaguuu uaaaaggcca acuaauaacc uuacuacacc augcgaaaaa cugugaagcc     3660

ucagacggua aaucccucga uuauaucgaa ucuaauagag aaauguuugg ugaacuuuug     3720

gcgcacgucu cagaauuugc uaagcgauac acacuagcag augcuaaccu uucuaaaauu     3780

aaccaauuau ucgagcagaa caaggacaac gacaucaaag uaauagcaca aucauuugua     3840

aacuuaaugg cauucaaugc uaugggugca ccagcaaguu ucaaguucuu ugaagcaaca     3900

auagagcgca aacgauacac aaaccuuaaa gaauuacuau cggcaaccau aauauaccaa     3960

uccauaaccg gacuauauga agcaagaaaa agacuugacg guuga                     4005


<210>  5277
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5277
augaaaaacc cuuauacuau uggccuagac auaggcacga acagcguagg augggcaguu       60

cuaacuaacc aauaugaccu aguaaaaaga aaaaugaaag uagcaggaaa cucugacaag      120

aaacaaauca agaaaaauuu cuggggagua agacuuuuug augacgguca aacagcaguc      180

gacagacgaa ugaaucgaac ugcuagacgc cgcauagaac gaagaagaaa ucgaauuuca      240

uaucuacagg aaauuuucgc aguagaaaug gcaaauauag acgcaaacuu cuuuugcagg      300

uuaaaugacu cuuucuacgu agauagugaa aaacgaaauu ccagacaucc auucuuugcu      360

acaauugaag aagaaguagc guaucaugac aacuacagaa caauauacca uuuaagagaa      420

gaauuaguua acaguagcga aaaagcagau cuacgccugg ucuauuuagc uuuagcacac      480

auaauaaaau accgcggaaa uuucuuaaua gaaggcgcgc uugacacuaa aaacacaucc      540

guggacgagg uauauaaaca auucauacag acauauaacc aaguauucau gagcaauauu      600

gaggaaggug cauuagcuaa aguagaagaa aaccucgaag uagcaucaau acuagcaggu      660

aaauuuacgc gaagggaaaa auucgaacgg auucuacgac uauauccggg agaaaaauca      720

acagguaugu ucgcucaauu cauuucucua auaguaggaa acaaagguaa cuuucaaaaa      780

gucuuuaauc ugguagagaa aacagacaua gaaugcgcaa aggauagcua ugaagaagac      840

uuagaagcuc uacuugcaau uauaggcgau gaguacgcag aauuauuugu ggcagccaaa      900

aacacauaca augcaguagu auuaaguagc aucauaacag uuacggccac agaaacaaau      960

gcaaaauuaa gcgccucuau gauagaacgg uuugaugcac augaaaaaga ccuaggagaa     1020

cuaaaggcuu ucauuaaacu ccacuugccc aaacaauacc aagaaauauu uaauaaugcu     1080

gcaauagacg gauacgcagg auacaucgau ggaaaaacaa aacaagucga uuuuuacaaa     1140

uaucuaaaaa caacguuaga aaacguagaa ggagcagauu auuuuauaac aaaaauagaa     1200

gaagaaaacu ucuuaagaaa acaaagaacc uucgacaacg gaguaauacc acaccaacuc     1260

cauuuagaag aacuugaagc aauauuacau caacaggcaa aauauuaccc cuucuuaaga     1320

gaagacuacg agaaaauaaa auccuuaguc acauuuagaa uaccauauuu uguagggcca     1380

cuagcaaaag gacaaucaga auuugccugg cuaacccgaa aagcugacgg agaaauuaga     1440

ccauggaaca uagaagaaaa aguagacuuc ggaaaaucag ccguagacuu cauagagaaa     1500

augaccaaca aagauaccua ucuacccaaa gaaaacguac uaccaaaaca uagucugugu     1560

uaucaaaaau acauggugua caacgaauua acuaaaguga gauacauuga ugaccaaggu     1620

aaaacaaauu acuuuagcgg ucaagagaag cagcaaauau ucaaugaucu cuuuaagcaa     1680

aaacgaaagg uaaagaagaa agauuuagaa cuuuucuuga gaaauauuaa ucacauagaa     1740

agcccaacua uugaaggacu agaagauagc uuuaacgcuu cauacgccac uuaucacgac     1800

cuacugaaag uagguaugaa acaagagagc cuagauagcc cucuaaaugc cgaaaugcua     1860

gaagauaucg ucaaaaucuu aaccguauuc gaagauaaaa gaaugauaaa agaacagcua     1920

caacaauuca gcgacguacu ggacggcguc gugcuaaaga aauuagaaag aagacacuac     1980

acaggcuggg gucggcuaag ugccaaauug cuagugggca uucgagacaa acaaagucau     2040

uuaacuauau uggauuaccu caugaaugac gauggacuaa aucgaaaucu caugcaacuc     2100

auaaacgauu ccaaucuauc auucaaauca auaaucgaaa aagaacaagu uaguacgacu     2160

gauaaagacc uacagucaau uguagcagac cucgcuggga gucccgccau aaagaaaggu     2220

auauuacaaa guuuaaaaau aguagacgaa cugguaagua uaaugggaua cccaccucaa     2280

accauuguug uagaaauggc ccgagaaaac caaaccacag uaaaagguaa aaacaauuca     2340

agaccacgau auaaaucacu agaaaaagcg auaaaagagu ucggaaguca aauacucaaa     2400

gaacauccua cugacaauca ggaacuaaag aauaaucgcc uauauuuaua uuacuuacaa     2460

aacgguaaag auauauacac aggacaagaa uuagacauac acaacuugag caacuaugac     2520

aucgaccaua uuguuccaca aaguuuuauu acagacaacu caauugacaa ccuuguacua     2580

acaaguagcg cuggaaauag ggaaaaagga ggcgacguac caccuuugga aauaguacga     2640

aaacgaaaag uguuuuggga aaaauuguau caaggcaacc uaaugagcaa aagaaaauuc     2700

gacuauuuaa ccaaagcaga gcgaggugga uuaacagacg ccgauaaagc gagguucaua     2760

caccgacaau ugguagaaac ccgacagauc acuaaaaacg uagcaaacau acuacaucaa     2820

agauuuaaca augaaacaga uaauuacggc aauaccaugg aacaaguacg aauaguaacc     2880

cugaaauccg cauuaguaag ucaauuucga aaacaauuuc aauuauacaa aguaagagaa     2940

guaaacgauu accaccacgc ucacgaugca uaccuuaaug gaguuguagc caacacuuua     3000

cugaaaguau auccacaacu agaaccagaa uuuguauacg gagaauacca ccaauuugau     3060

ugguucaaag caaacaaagc uacagcuaag aaacaauuuu acacuaauau aauguuauuu     3120

uucgcccaaa aagagagaau aauagacgag aauggugaaa uacuauggga uaagaaauau     3180

uuagaaacca uaaagaaagu gcuagauuac agacaaauga acauugucaa gaaaacggaa     3240

auucaaaaag gcgaguucuc aaaagcuacu auaaaaccaa aagguaauuc uucaaaacua     3300

auaccaagaa aagaaaauug ggacccaaug aaauauggag gauuagacag cccaaacaug     3360

gcauaugcug uaauuauaga acaugcaaaa ggcaaaaaga aaauagucau agaaaagaaa     3420

uuaauucaaa uaaacauuau ggagcguaaa auguucgaaa aagaugaaga ggccuuucuu     3480

gaagaaaaag gauaccgaca uccaaaaguu uuaacaaaac uaccaaaaua cacguuauac     3540

gaaugcgaaa aaggcagaag acgaaugcuc gcauccgcaa augaagcuca gaaagggaac     3600

caauuaguac ucucuaacca ccugguaagu cuacucuauc augcgaaaaa uugcgaagcc     3660

uccgacggaa aaucacuaaa guauauagaa gcacaucgag aagcguucuc agaacuacuc     3720

gcacaaguua gcgaauuugc aacucgauac acacuagcag augcaaacuu aagcaaaauu     3780

aauaacuuau ucgaacaaaa caaagaaggc gacauaaagg cgauagccca aucauuuguu     3840

gaucuaaugg ucuucaaugc aaugggcgcu ccagcguccu uuaaauuuuu cgaggcaacc     3900

auagauagga aaagauacac aaaucuaaaa gaauuacuau caucgaccau aauuuaccaa     3960

ucgaucacug gacuauauga gucaagaaaa aggcucgacg acuga                     4005


<210>  5278
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5278
augaaaaauc cauacacaau aggauuagau auagguacaa auagcguagg augggcugua       60

cuaacggacc aauaugaccu gguaaaacgg aaaaugaaag uagcaggaaa cagugacaag      120

aagcaaauaa aaaaaaacuu uuggggggua cguuuauuug augaaggcga gacagcggcu      180

gauagacgaa ugaaccguac ugcaagacga cgcauugaac gacgacgaaa uagaauauca      240

uacuuacaag aaauauuugc acuagagaug gcaaacaucg augcaaacuu uuucugucga      300

uugaacgaca guuuuuaugu ugacucagaa aagagaaacu cuagacaucc auuuuuugcc      360

acaauagaag aggaaguagc cuaccacaaa aacuacagaa caaucuacca uuuacgagag      420

gagcuaguaa acucgaguga aaaagcagac cuccguuuag uguacuuagc ucucgcacau      480

auaauaaaau auagagguaa uuucuuaaua gagggagcac uagauacuaa aaacacaagc      540

guugacggag uauauaaaca auucauucag accuacaacc aaguauucau aucaaauaua      600

gaagagggaa cauuagcaaa gauggaagag aacacuacag uagcuaacau acucgccggg      660

aaauucacga gaaaagagaa auuggaaaga auacuacaau uguaccccgg agaaaaaucc      720

acaggcaugu ucgcacaguu uaucucacua auagucgguu caaaagggaa uuuucaaaaa      780

guauucgauc uuguagaaaa aacagauauu gaaugcgcua aagacaguua ugaagaagau      840

cuagaagcac uacuagcaau aauaggcgau gaguaugcug aauuauucgu agccgcaaaa      900

aauacuuaca augcgguagu acuaaguuca auaauaacag uaacagauac agaaacuaau      960

gcgaaacuuu cagcaucaau gaucgaaaga uucgacgccc acgaaaaaga ccuaagugaa     1020

uuaaaagccu ucauaaaauu acacuuacca aaacaauacg aagaaauauu uucgaacgua     1080

gcaauagacg gauaugcugg cuacauagac ggaaaaacca aacaaguaga cuucuacaaa     1140

uauuuaaaaa cauuacuaga aaacauagaa ggagcggauu acuuuauagc aaaaauugaa     1200

gaagaaaauu uucucaggaa acaacguaca uucgacaacg gugcaauccc ccaucaguua     1260

cacuuggaag aacuagaagc aauacuccau caacaagcga aguacuaccc auuccugaaa     1320

gaagcuuaug auaaaauaaa aucacuagua acauucagaa ucccauauuu uguagggccu     1380

cucgcaaacg ggcaaucuga cuuugcaugg cuuaccagaa aagcggaugg agagauacgc     1440

ccauggaaca uagaagaaaa aguagauuuc ggaaaaagcg cugucgacuu cauugaaaag     1500

augacgaaca aagacaccua ccuuccuaag gaaaaugugu uacccaaaca caguuuauau     1560

uaucaaaaau auaugguaua uaacgaacua acgaaaguua gauauauaga ugaccaaggu     1620

aaaaccaacu auuuuucagg acaagaaaaa caacaaaucu uuaaugacua uuucaagcaa     1680

aaacguaaag uaucaaaaaa agacuuagaa caauucuuaa gaaacauguc gcauauagaa     1740

agcccaacaa ucgaagggcu agaagauuca uuuaauucuu cauaugcgac auaucaugau     1800

uuauuaaaag ucggaaucaa acaagaagua cuugaaaacc cccuuaacau ggaaauguua     1860

gaagauauag ucaagauacu aacagucuuc gaggacaaaa gaaugauuaa agaacaauua     1920

caacaauuuu ccggaguccu agacggcgca guauuaaaaa aacuagaaag acgacacuac     1980

acaggauggg gaagguuauc agccaaacua uuaguaggaa uacgggauaa acaaucccau     2040

cuaacuauac uagauuauuu aaugaacgau gauggauuga acagaaaccu aaugcaauua     2100

aucaacgaca gcaacuugag cuuuaaauca auaauagaaa aagagcaagu uagcaccaca     2160

gacaaagacc uacagagcau aguagcagac uuagccggaa gcccagcaau aaaaaaaggc     2220

auacuucaaa gucuaaaaau aguagacgaa cuaguaagua uaauggggua ccccccacag     2280

acgauagucg uggaaauggc aagagaaaac caaacaaccg uuaaaggaaa aaauaacuca     2340

agaccaagau auaaaagccu ugaaaaagcg auaaaagaau uuggauccca aauccuaaaa     2400

gagcacccaa cagauaacca ggaacuccga aauaauagau uauaccuaua cuaucuacag     2460

aauggcaagg acauguauac aggucaggaa uuagauauac auaaccugag uaacuacgac     2520

auagaccaua ucgucccgca aucauuuaua acagacaacu ccauagauaa ccucguauua     2580

acaaguucag ccgguaaucg agaaaaagga gaugacguac caccacuaga aauaguccga     2640

aaacgaaaag uuuucuggga aaaacuuuuu caggggaauc uuaugucaaa acgaaaauuc     2700

gauuaccuga ccaaagcaga acgaggcggg cuaacagaag cagacaaagc gacguucaua     2760

cauagacaac uugucgagac ccgacagaua acaaaaaacg uggcaaauau auuacaccag     2820

agguuuaaca acgaaacuga caaccacgga aauaacaugg agcaaguaag aauaguaaug     2880

cuuaaaagcg cauugguguc gcaauucaga aaacaauuuc aauuauauaa aguaagagag     2940

guaaacgacu aucaccaugc acacgacgcu uauuuaaaug gaguuguggc aaauacuuua     3000

cucaaagucu acccacaacu ugaaccagaa uuugucuacg gagaauacca ucaauucgac     3060

ugguucaaag caaacaaagc gacggccaag aaacaauucu acacaaauau caugcucuuu     3120

uuugcccaaa aagaaagaau aaucgaugaa aauggagaaa uucucuggga uaagaaauac     3180

uuagaaacua ucaaaaaagu auuagacuau agacaaauga auauaguuaa aaaaaccgaa     3240

auucaaaaag gggaauucuc aaaagcaaca auuaaaccga aagguaacuc aucaaaacuu     3300

auaccaagga aagagaauug ggauccaaug aaauauggag gacuagacuc uccaaauaug     3360

gcauacgcgg ucauaauaga acaugccaag gguaaaaaaa aaauaguuuu ugaaaaaaag     3420

auaauaagaa uuacaauaau ggaacgaaaa gcauucgaaa aggaugaaaa aucauuccuu     3480

gaaaaacaag gauauagaca accaaaagua cuaaccaaac uaccaaaaua uacacuauau     3540

gaaugcgaaa auggucgacg aagaaugcua gcaucagcca acgaagccca aaagggaaau     3600

caacaaguau uaaagggaca acuuauaaca cugcuacauc acgcaaaaaa cugugaagcc     3660

ucggacggaa aaucgcucga uuauauagaa aguaauagag aaauguuugg ugagcuucua     3720

gcccacguau cagaauuugc uaaacgauau acucuagcag augcaaaucu cucaaaaauc     3780

aaucagcucu ucgaacagaa uaaagauaau gacaucaaag uuauagcuca aucuuuugua     3840

aacuuaaugg cguuuaaugc aaugggggca ccagcaagcu ucaaauuuuu cgaggcaacc     3900

aucgagagaa aacgauauac gaacuuaaaa gaacuauugc gagcuacuau cauauaucag     3960

agcauaacug gacucuauga agcacgaaaa agacuagaug gauga                     4005


<210>  5279
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5279
augaaaaauc cuuauaccau aggacuagac auagggacua auuccguagg uugggcagua       60

uugacugauc aguacgacuu aguaaagcgc aaaaugaagg uugcaggcaa uagcgauaag      120

aaacaaauua agaaaaacuu uuggggaguc cgccuauucg acgaagguga gacagcagca      180

gacaggcgua ugaauagaac agcaagacgc cgcauugaaa gaagaaggaa uaggaucuca      240

uaccuacaag aaauuuucgc ccuugaaaug gcuaauauag acgcgaauuu cuuuugcaga      300

uugaaugaca gcuuuuaugu agauucugag aaaagaaauu ccagacaccc auuuuucgca      360

accauugaag aagaaguagc auaccauaaa aacuauagga cuaucuauca ccuuagagaa      420

gaauuaguua acaguaguga gaaagccgac cuaagauuag ucuaucuagc acuagcacau      480

auuauaaaau acagaggaaa cuuuuugaua gaaggagcau uagauaccaa aaauaccagu      540

guggacggug uauauaaaca auuuauccaa acuuauaauc aaguuuucau aucaaauaua      600

gaggaaggga cucuagcaaa aauggaagaa aauacuacug uagcagacau acuagcagga      660

aaauucacua gaaaagaaaa auuagaaaga auacuucaau uauacccggg ggaaaaauca      720

acaggaaugu uugcgcaauu cauaagucua auaguaggaa guaaaggaaa uuuucagaaa      780

guuuuugauc uaguagagaa aacugacaua gaaugcgcua aagacucaua ugaggaggac      840

cuagaagcuc ucuuagccau aauaggagac gaauacgcag aauuauucgu agcagcuaaa      900

aacacauaca augcaguagu ccuuuccucc auaauaacag uaacagacac cgaaacaaau      960

gcaaaacuuu cugcaaguau gauagaaaga uucgacgcac augagaaaga ucuuagugaa     1020

uuaaaagcgu uuauaaaauu acaucuaccg aaacaauacg aagaaauauu cucuaaugua     1080

gccauagacg gauaugcagg auauaucgac ggaaaaacaa aacaaguaga cuuuuauaaa     1140

uaccuuaaaa ccuugcuaga aaacauagaa ggagcagacu acuucauagc aaaaauagaa     1200

gaggaaaacu ucuuacgaaa acaacggaca uucgacaacg gagcaauacc ccaccaacuc     1260

cauuuagagg aacuugaagc uauacuacac caacaagcua aauacuaccc cuuucuuaaa     1320

gaagcauacg acaaaauaaa gagcuuggua acguucagga uuccuuauuu cguaggaccg     1380

cuugcaaacg gccaaucaga uuuugcaugg cuaaccagaa aagcagacgg agaaauaaga     1440

ccuuggaaca uagaagaaaa aguagacuuu gguaaaucug caguagacuu uauagagaaa     1500

augaccaaua aagacacaua ucugcccaag gaaaauguau uacccaagca uucauuguac     1560

uaucaaaagu auauggucua caacgaauug acaaaaguac gcuacauaga ugaucaagga     1620

aaaacaaacu acuuuuccgg gcaagaaaaa caacaaauau uuaacgacua cuucaaacag     1680

aaaagaaaag uaucaaagaa agaccuugaa caauuccuca gaaacaugag ucacauagaa     1740

ucacccacga uagaaggacu agaagauagu uuuaacucuu cauaugcaac auaucaugau     1800

cuucucaaag ucggcauaaa acaagaaguu cuagaaaacc cacuaaacac agaaaugcua     1860

gaagacauag uuaaaauacu aacuguuuuc gaagacaaaa gaaugauaaa agaacaacua     1920

caacaguuuu cagacgucuu ggacggugcu guccucaaga aauuggaacg ccggcacuac     1980

acaggauggg gacgauuguc agcaaaacua uuaguaggua ucagagauaa acaaucacau     2040

uuaacuauuc uagauuaccu aaugaacgau gauggccuaa accgcaacuu aaugcaacuu     2100

auuaaugauu caaaccuauc auucaaaagc auaauagaaa aagaacaagu aucaacuaca     2160

gacaaagauc uacaaaguau aguagcagau cuagcaggga gcccagcaau caagaaagga     2220

aucuuacaau cuuuaaaaau aguagaugaa cuuguaucaa uuaugggaua ucccccgcaa     2280

acuauugugg uagaaauggc gcgcgaaaac caaacaacug uaaaagguaa aaacaacucu     2340

cgaccuagau acaagagcuu agaaaaagca aucaaagagu ucggcagcca aauacuuaaa     2400

gaacacccua cagacaacca ggaauuacgc aacaaccgcc uauaccucua uuacuuacaa     2460

aauggaaaag acauguauac aggccaggaa cucgauauuc auaaucuauc aaauuacgac     2520

aucgaucaca ucguacccca aaguuuuauc acugacaauu caauagacaa ccuuguccua     2580

acaaguagcg cuggaaacag agaaaaagga gaugauguuc caccuuuaga aauaguaagg     2640

aaacgaaaag ucuucuggga aaaauuauuc cagggaaauu uaaugaguaa gagaaaguuc     2700

gacuauuuaa caaaagcuga acgaggcgga uuaacagaag cagacaaagc uaccuuuauc     2760

caccgacagc uagucgagac ucgucaaaua accaaaaacg ucgcaaacau acuccaucaa     2820

cgauucaaca acgagacgga uaaucauggu aacaacaugg aacaaguaag aauagugaug     2880

cuaaaaucug cucuaguuuc gcaauuccga aaacaauucc aauuguauaa aguuagagaa     2940

guaaacgacu accaucacgc ucaugaugca uaccuuaacg gaguugucgc aaacacccua     3000

cugaaaguau auccacaauu ggaaccggag uuuguuuacg gagaauauca ucaauucgau     3060

ugguuuaaag caaauaaagc uacagcuaaa aagcaauucu auacaaacau aaugcuuuuc     3120

uucgcacaga aagaaagaau aauagaugag aauggcgaaa uauuauggga caagaaauac     3180

uuagaaacua uaaagaaagu cuuagauuac agacaaauga acaucguaaa gaaaacagag     3240

auccagaaag gugaauuuag caaagcaaca auuaaaccaa aaggcaacuc aaguaaauug     3300

auaccaagaa aagaaaacug ggauccgaug aaauauggcg gacucgauuc uccaaacaug     3360

gcauacgcag ucauaauaga acacgccaaa ggcaaaaaga aagugguauu ugagaagaaa     3420

auaauaagaa uaacgaucau ggagagaaaa gcauuugaaa aagaugaaaa gagcuuccua     3480

gagaaacaag gauacagaca accaaaaguu cuuacaaaac ucccaaaaua cacacuauau     3540

gaaugcgaaa acggacgacg acgaaugcug gcaucagcaa augaagcaca aaaaggaaau     3600

caacaaguuu uaaaaggcca acuaauaaca cuccuacacc acgccaaaaa uugugaagca     3660

ucagacggga aaucauuaga cuacauagaa aguaaccgcg aaauguucgg agaacugcua     3720

gcacaugucu cagaauuugc aaaaagauac acacucgccg acgcaaacuu aaguaagaua     3780

aaccaauugu uugagcaaaa uaaagacaac gauauaaaag uaauugcaca aaguuucguc     3840

aaccuaaugg cauuuaacgc caugggagca ccggccucau ucaaauuuuu cgaagcuacc     3900

auugaaagaa aaagauacac aaaucuuaaa gaacuauuaa gugcaacgau uauauaucaa     3960

agcauuacug gcuuauauga agcaagaaaa agacucgaug gauga                     4005


<210>  5280
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5280
augaaaaacc cauacacuau cggacuagac auagguacca auuccguugg augggcagua       60

uuaaccaacc aauaugacuu ggucaaaaga aaaaugaaag uagcugguaa uucggacaag      120

aaacaaauaa agaaaaacuu uuggggaguu cgccuguuug augaugguca aacggcaguc      180

gaccguagaa ugaacagaac agcgagaaga agaaucgaaa gaagaaggaa cagaauaucc      240

uaucuacagg aaaucuuugc uguagaaaug gccaacauug acgcgaacuu cuuuugcagg      300

cuuaacgacu cauucuaugu ugauucagag aaaagaaaua gccgacaccc guucuuugca      360

accauagaag aagaaguagc auaccaugac aacuaucgaa caauauacca uuuacgcgaa      420

gaacucguaa acagcucuga aaaagcagac cuaaggcucg ucuacuuggc acuagcgcau      480

aucauaaaau acaggggcaa cuuuuugauu gaaggggcuc uggacacgaa aaauacaagu      540

gucgacgaag uauauaaaca auuuauacaa acauacaacc aagucuucau gucaaacaua      600

gaagaagggg cccuagcuaa aguugaagaa aacuuagaag uggcaagcau ucuagcaggu      660

aaauuuacac gacgagaaaa auuugaaagg auucuuagac uauacccagg agaaaaaucu      720

acuggaaugu ucgcacaauu uauaagucuc auuguaggaa acaaaggaaa uuuucaaaaa      780

guguuuaacc ucguagagaa aacagacaua gaaugcgcaa aagauuccua cgaagaagau      840

uuagaagccu uauuggcaau aaucggcgac gaauaugccg aauuauucgu agccgcgaaa      900

aauacauaca acgcaguagu ccucucgagc auaaucacag uaacggcuac cgaaacaaau      960

gcaaaauuau cagccucuau gauagaaaga uucgacgcac acgaaaaaga ccuaggggaa     1020

cuuaaggcuu ucaucaaacu ccaucuacca aaacaauauc aagaaauauu caauaacgcu     1080

gcgauagacg gauacgcagg auauauagac ggcaaaacaa aacaagugga uuuuuauaaa     1140

uauuuaaaaa caacacuaga aaacguagaa ggagcggauu acuuuauuac gaaaaucgaa     1200

gaagaaaacu ucuuaagaaa acaaagaacc uuugacaacg gaguuauccc acaucaguua     1260

caucuagaag aauuagaagc aauauuacau caacaagcaa aauauuaucc cuuccuucgc     1320

gaagacuaug aaaagauuaa aucauuagua acguuuagaa uacccuauuu cguaggaccu     1380

uuagcuaaag gccaaucuga guucgcaugg uugaccagga aagccgaugg cgaaauaaga     1440

ccguggaaua uagaagaaaa aguagacuuc ggaaaauccg ccguugacuu cauugagaaa     1500

augacaaaua aagauacaua uuuaccaaaa gaaaacguau uaccaaaaca cucuuuaugu     1560

uaccaaaaau acaugguaua caaugaacua acaaaagugc guuauauaga cgaccaaggc     1620

aaaacaaauu auuuuagugg gcaagaaaag caacaaauau ucaacgauuu auuuaaacaa     1680

aaacgaaaag uuaaaaagaa agauuuagag cuauuccuaa gaaauauuaa ccacauagaa     1740

uccccaacca uugaaggacu cgaagacuca uucaaugcgu cauacgcuac auaccacgac     1800

cuacuuaaag uagguaugaa acaagaauca cuagauagcc cacucaaugc cgaaauguua     1860

gaagauauag uaaaaauuuu aacaguauuc gaagacaaaa gaaugauaaa agaacagcuc     1920

caacaauuua gugauguccu agacggcgua guguugaaaa agcuagaacg aagacauuau     1980

acaggguggg gccgacuauc ugcaaagcua uuaguaggca uaagggauaa acagucacau     2040

uuaaccauac uagacuaccu caugaacgau gacggauuaa accgaaacuu gaugcaacuc     2100

auaaaugauu caaaccuaag uuuuaaaagu aucaucgaaa aggaacaggu caguacaacc     2160

gacaaagauu uacaaucuau uguagcggau cuagcaggaa gcccugccau uaaaaaggga     2220

auauuacaga gcuuaaaaau agucgaugaa uuaguuucaa uaaugggcua uccuccccaa     2280

acaaucguag uugaaauggc acgagaaaau cagaccacag uaaaaggcaa aaauaacuca     2340

agacccagau acaaaagccu agaaaaggca auaaaagagu ucggaucaca gauccuaaaa     2400

gaacauccaa cugauaauca ggaacuuaaa aacaaccggu uauaccuuua uuacuuacaa     2460

aauggaaaag acauauauac aggacaagaa uuagauauac acaaucucuc caacuacgac     2520

aucgaccaca uaguuccuca aucauucaua acagauaauu cgaucgacaa ucuagugcua     2580

acaucaucag caggaaaucg ugaaaaaggc ggggaugucc caccacuuga aauaguacgg     2640

aagagaaagg ucuucuggga gaaacuguac caaggaaacu uaaugaguaa aagaaaauuu     2700

gacuacuuaa caaaagcaga aagaggugga cuaacugacg cagacaaagc ccgauuuaua     2760

caucgacaac uuguagaaac acgacaaaua accaaaaaug ucacaaauau acuacaccaa     2820

agauuuaaua acgaaacaga uaauuacggc aacacuaugg agcaagugag aauugugacc     2880

cuaaaaagcg ccuuaguaag ccaauuucgg aaacaauuuc aacucuacaa aguucgagaa     2940

gugaacgauu accaucaugc ccaugacgcc uaucuaaaug guguaguugc uaauacauua     3000

uugaaaguau acccgcaacu agaaccagaa uuuguuuaug gagaauauca ccaauuugau     3060

ugguuuaaag cuaacaaagc aaccgcaaag aaacaauuuu acacaaauau aauguuauuc     3120

uucgcacaaa aagagagaau aauugacgaa aauggcgaaa uuuuguggga uaagaaauac     3180

cuagaaacua uuaagaaagu auuagacuac agacaaauga acauaguuaa gaaaacugaa     3240

auacaaaaag gagaguucag uaaagcaaca auuaaaccga aaggaaacag uagcaaacua     3300

auaccaagaa aagaaaacug ggaucccaug aaauacgggg gauuggacuc accaaacaug     3360

gcuuaugccg ucauaauaga acacgcaaaa ggcaaaaaga aaauaguuau agaaaagaaa     3420

uuaauccaaa uaaacauaau ggaaaggaaa auguucgaaa aagacgaaga agcauuucuu     3480

gaagaaaaag gcuacagaca uccuaaagua uuaaccaaau uaccaaaaua cacauuguac     3540

gaaugugaaa aaggacgcag acgcaugcua gcauccgcaa acgaagcaca aaaaggaaau     3600

caacucguac uaucaaacca ucuagugagu uuacucuacc acgcgaaaaa cugcgaagcc     3660

ucugacggua aaucucuuaa auacauagaa gcccaccggg aagcauuuuc cgaauuauua     3720

gcacaaguaa gugaguucgc aacaagauau acccuagcag augcuaaccu aucaaaaauu     3780

aacaaccuuu ucgaacaaaa caaagaaggu gacauaaaag ccauugcaca gucuuuuguc     3840

gaucuaaugg uguucaacgc uaugggagcu ccagccucgu ucaaauucuu ugaagcgacu     3900

auugaucgaa aaagguauac aaaccuaaaa gaacuacuau caaguacaau aauauaucaa     3960

uccaucacag gccuuuauga aucaaggaaa aggcuagaug auuga                     4005


<210>  5281
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5281
augaaaaagg aauauacaau uggauuagau auaggaacaa acaguguggg gugggcaguc       60

cuuaccgaaa acuaugacuu aguaaaaaaa aagaugaaag uauacggcaa cacggaaacc      120

aaauaccuua aaaaaaauuu auggggagua cgucuauucg augaaggaga aacggcagca      180

gaucgccguc uuaaacgaac aacaagaagg agauacaguc gaaggcgaaa ccgaauaugc      240

agacuacaag acuuauuuac ugaagaaaug aaucaagugg augcaaacuu uuuccaucgg      300

cugcaagaaa guuuuuuagu cccagacgaa aaggaauucg aacgacacgc caucuuuggu      360

aaaauggaag aagaaguauc uuauuaucgu gaauuuccca ccauuuacca ccuucgaaaa      420

cacuuagcag acacuucaga acaagcagau uuaagauuag ucuacuuagc cuuagcucau      480

auuguuaaau auaggggcca uuuccuuaua gaaggagaac uaaauacgga aaauucuucu      540

gugucagaaa cauuucgaac auuuauucag guauauaacc agaucuucag agaaaacgaa      600

gucccacuag cagucccaga caauauagaa gaguuauucu cagaaaaagu uagucgcgca      660

agaaaaguug aggcaauacu aucagucuac agugaagaaa aaaguacggg aacgcucgca      720

caauucuuga aauuaauggu aggaaaucaa ggaagauuca agaaaacauu ugacuuagag      780

gaggauggca ucauacaaau acccaaagag gaauaugaag aagaacuaga aacuuugcuc      840

gcuaucauug gugacgaaua cgcagaaauc uucucagcua cgaaauccgu uuacgaugcc      900

guagcuuuau ccggaauucu gucagugacc gauggagaua ccaaagccaa acuaucagcu      960

ucaaugguag aacgauacga agcucaccag aaagaucuag uacaauucaa acaauucaua     1020

agaaaagaac ucccugaaau guaugccccc auauucagag auaacucagu aaguggauac     1080

gcaggauaug uagaaaauag caaaguaguu acacaagcag aauuuuacaa auacauaaag     1140

aaagcaauag aaaagguccc aggugcagaa uacuuuuuag aaaaaauuga acaagaaacc     1200

uuuuuagaua aacaacguac auuuaacaau ggcgugauac cacaucaaau ccauuuagaa     1260

gaacuagaag caauuauuca aaagcaagcc acuuauuacc cauuccucgc agacaacaaa     1320

gaagagauga aacaacucgu aaccuuuagg auacccuacu auguaggacc ccuugcagau     1380

gggaauucuc cguuugcuug gcuagaaaga auuagcuccg aaccaauacg cccaggaaau     1440

cuagcagaag ucguugauau uaaaaaaucc gcuacaaaau uuauugaaag aaugacuaac     1500

uucgacacgu aucucccaac agaaaaaguu uuacccaaac acucaaugau uuacgaaaaa     1560

uauaugguau acaaugaauu aacaaaggua ucauacguag augaacgggg aaugaaccaa     1620

cgcuuuucgg gagaagaaaa aaagcaaaua guagaagagu uauuuaaaca aucuagaaaa     1680

guaacaaaaa aacuauuaga aaaauuccug ucaaaugagu ucggacuagu cgaugucgcu     1740

auaaaaggca uugagacauc uuucaaugca ggauacggaa cauaccacga cuuucuaaag     1800

auagguauca ccagagaaca acuugauaaa gaggaaaaua gugaaacacu agaggaaauc     1860

guuaaaauuu uaacuguuuu cgaagaccga aaaaugauac gagaacaacu uaaaaaauac     1920

acguaucucu ucgaugagga aguacuaaaa aaacuagagc gacgacacua uacgggaugg     1980

gguagacuaa gcgcaaaauu acuaauugga aucaaagaaa aacgaaccca uaaaacaauc     2040

cuagacuauc ugauagaaga cgacggcggc aaacaaccaa ucaaccguaa ucucaugcaa     2100

uuaauaaacg acucugauuu aucauuuaaa agugaaauag cugaagcaca aucugacaug     2160

aauacagaag accuucacga aguaguacaa aacuuagccg guucuccagc uauaaaaaaa     2220

ggcauacuuc aaagucuaaa gauagucgac gaauuagugg auaucauggg aucacuuccu     2280

aaaaacauag uaguagaaau ggcaagagaa aaccaaacua caagccgagg acguaccaac     2340

ucaaauccca gaaugaaagc acuagaagag gcaaugagaa acuuaagaag caaucuacua     2400

aaagaauacc cgacggauaa ccaagcucua caaaaugauc gauuauaccu auacuacuua     2460

caaaacggaa aagacaugua uacuggucua gaccuaucuc uacacaauuu aucaaguuac     2520

gauauagauc acaucguacc acaaaguuuc acaacggaca acucacucga caaucgcguu     2580

cuuguuucau ccaaagaaaa ucgcggaaaa aaagacgaug ucccaaguaa agaaguggua     2640

caaaaaaaua uaacucuuug ggaaacacua aaaaauucga accuaaucuc acagaaaaag     2700

uaugacaauc ugaccaaagg acuaagggga ggauuaacug aagacgacag agcucauuuu     2760

auaaaaagac aacuaguaga aacaagacaa aucacuaagc acguagcaag aauauuggau     2820

caacgauuua acagucaaaa agacgaagaa ggaaaaacaa ucagagcagu ccgagucgua     2880

accuuaaaaa gcucacuuac aagucaauuc cguaaacaau uugcaauaca uaaagucaga     2940

gaaauaaaug acuaucauca uggucaugac gcguaccuca acgguguagu agcuaacagu     3000

cuacuacgag uauacccgca acuccaacca gaauucguuu acggagauua cccaaaguuu     3060

aaugccuaca aagcaaauaa ggccacagca aaaaaacaau uauauaccaa uaucaugaaa     3120

uucuuugcag aggacgcagu aauaauagau gaaaacggag agaucuuaug ggauaaaaag     3180

aauauugcaa caguaaaaaa aguaaugucc uacccucaga ugaauaucgu aaaaaaaccc     3240

gaaauccaaa caggaucuuu uagcaaggaa acaauuaaac cuaagggaga uucugacaaa     3300

cucauaucaa gaaaaaccaa cuggucgccu aaacuauacg gaggauuuga uucaccccaa     3360

guagcauacu caguaauaau aacauacgaa aaagguaaaa aaaaaguacg ugcgaaagca     3420

auaguaggca uuacaaucau ggaacaauca uuauucaaga aagauccagu aucauuauua     3480

gaagaaaagg gauacgcaaa cccugaagua uuaauucacc ugccaaaaua caccuuauau     3540

gagcucgaaa acggacgucg uagacuacua gccagcgcga acgaagcaca aaaaggcaau     3600

caacuagucc uaccagcguc ucugguaaca cuacuguacc acgcgaaaca aguagacgaa     3660

gacaguggaa agucagaaga auacguacga gaacaccgug cagaauucgc cgaaauacua     3720

aacuaugucc aagcauuuag cgaaaccaaa auacuagcca acaaaaaucu ccaaaccauc     3780

cuaaaacuuu augaagagaa caaggaagcu gacaucaaag aaauugccga gaguuuuguu     3840

aaccuuauga aauuuucagc auacggagca ccaauggauu ucaaauucuu uggaaaaacg     3900

auuccgagau ccagauacac uucgguaggc gaacucuuau cugcuacaau aaucaaucaa     3960

aguauaacag gucuuuacga aacgagacgu aaacuaguag auuga                     4005


<210>  5282
<211>  4005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5282
augcguaaac cauacaccau aggacuagac aucggaacaa acagcguagg cugggcaguu       60

uugacagauc aguacaacuu gguaaaaaga aaaaugaaag uugcagguuc agccgaaaag      120

aaacaaauca aaaaaaacuu cuggggagua agacucuuug augaaggaga aguggcagca      180

ggacgacgaa ugaauaggac cacaagacgg agaaucgaaa ggcgacgaaa ccgaaucgcc      240

uaccuacaag aaauauuugc cgcagaaaug gccgaaguag acgcaaacuu uuuuuacaga      300

uuagaagauu ccuucuauau agaaucagaa aaacgacauu caagacaccc auucuuugca      360

acgauagaag aagaaguagc cuaccacgaa gaauacaaaa caauuuauca ccuacgagaa      420

aaauuaguaa auucgagcga caaagcggac uuaagauuag uauacuuggc guuggcacac      480

auaauaaaau auagaggaaa uuuucuaaua gagggaaugc uagacaccaa aaacacaagu      540

guagaugaag uauucaaaca guuuauacaa acauauaacc aaauauucgc uagugacaua      600

gaggaaggau cauuaaccag acuugaagaa aacaaagaag uagccgaaau acuaucugag      660

aaauuaacga gaagagagaa acuugauaag auacuaaaac uguacaccgg ugaaaaauca      720

acaggaaugu uugcaagguu cauuaauuua auaauuggcu cuaaaggaga cuuuaaaaaa      780

guuuucgauu uagacgaaaa agcagagauu gaaugcgcua aagauacaua cgaagaagau      840

uuagaagcau uacucgcaaa gauaggagac gaauaugcug aaauuuuugu cgcugcaaaa      900

ucaacauaua acgcaguagu acuuucaaac aucauaaccg uuacagacac ugaaacaaaa      960

gcaaaauuaa gcgcauccau gauagaacgu uucgacaaac acgcuaaaga ucuuaagaga     1020

uuaaaagcgu ucuuuaagau gcaacuaccc gaaaaauuua augaaguguu uaacgacauu     1080

gaaaaagacg gcuaugccgg auacauagac ggaaaaacga cgcaagaaaa auucuauaaa     1140

uacaugaaaa aaauguuagc aaacauagac ggagcugacu auuucauuga ccaaauagaa     1200

gaggaaaauu ucuuacgaaa acaaagaaca uuugauaacg gcacuauacc acaccaacua     1260

caucuggaag aauuagaagc uauccuacac caacaagcca aauacuaucc guuucuaaga     1320

aaagauuacg aaaaaauaag aucacucgua acauucagaa uaccauacuu cauaggucca     1380

uuagcaaaug gacaaagcga cuuugcaugg uugacaagaa aagcagacgg agaaaucaga     1440

ccauggaaua uagaagaaaa aguagacuuu ggaaaaucag caaucgacuu uauagaaaaa     1500

augacaaaua aagacacaua ucuaccaaaa gaaaacgugu uaccaaaaca uucacuaugc     1560

uaucaaaagu auaugguuua uaacgaauua acuaaaauca gguauaucga ugaccaagga     1620

aaaacacacc auuucucagg gcaagaaaaa caacaaauuu uuaauggguu auuuaaacaa     1680

caacguaaag uaaaaaaaaa ggauuuagag cgauucuugu auacaaucaa ucauaucgaa     1740

aguccuacga uagaaggcgu cgaagacgcg uuuaacuccu cauucgcuac cuaucacgac     1800

cuacaaaagg guggagugac ccaagaaaua uuagacaauc cccuaaacgc ugauaugcug     1860

gaagaaauag ucaaaauauu aaccguauuc gaagacaaac gcaugauuaa agaacaauua     1920

caaucuuucu cugacguucu ugacggaacu auccugaaaa aacuagagcg gcgucauuau     1980

accggcuggg gacgcuuaag cgcuaaacuc cuaacaggca uaagagacaa gcauagccau     2040

cuaacaaucu uagacuaucu aaugaacgac gauggcuuaa auagaaauuu aaugcaacuc     2100

auaaaugauu cuaacuuauc cuuuaaaucu aucaucgaaa aagagcaagu aucuaccgca     2160

gacaaaggca uucaaaguau agucgcugaa cuagcuggau ccccagcaau aaaaaaagga     2220

auacuucaau cccuaaaaau aguugaugaa uuaguaggaa uaaugggcua uccaccacaa     2280

acuauaguag uagagauggc ccgagaaaac caaacuacag ggaaaggcaa gaauaauagc     2340

aagccacgau uuaucucauu agaaaaagca auaaaagaau uuggcucaca aauccuuaaa     2400

gaacacccca ccgacaauca augcuuaaaa aaugauagau uguacuuaua cuaucuacaa     2460

aaugguaaag acauguacac agggaaagag cuagauaucc acaacuuguc aaauuaugac     2520

aucgaccaua uaauaccaca aagcuucaua acugauaacu ccauagacaa cagaguucuu     2580

guuucaucaa cagcaaauag agaaaaagga gauaaugugc cgcuacuaga agugguaaga     2640

aaaagaaaag ccuucuggga aaaauuauau caagccaaac uaauguccaa acggaaauuc     2700

gacuaccuaa caaaggcaga acgcgggggu uuaaccgaag cagacaaagc aaauuuuauu     2760

caaaggcaac uaguggaaac uagacaaaua acuaaaaacg uugccaauau acuauaccaa     2820

agauucaauu gcaaacaaga cgaaaacgga aacgaagucg aacaaguuag gauugucacc     2880

cuaaaaucaa cacugguauc acaauuuaga aaacaguucc aacuauauaa aguaagagaa     2940

guaaacgacu aucaucaugc acacgaugca uaccuuaaug ggguuguagc aaauacacuc     3000

uuaaagguuu auccacaacu agaaccagaa uucguauacg gcgauuauca ucaauucgac     3060

ugguucaaag caaauaaagc uacugcaaaa aaacaauuuu acacgaauau caugagauuc     3120

uucgccaaag agaaucaaau aaucgauaaa aauggagaga uacuauggga caacagauau     3180

cuagauacaa uuaaaaaggu auuaucauac agacaaauga auaucguaaa aaaaacggaa     3240

auucaaaaag gagaauuuuc caaugcaacu guaaauccca aaggcaacuc aagcaaacua     3300

auaagcagaa aagcugacug gaacccaaua aaauaugggg gauuugacgg aucuaacaug     3360

gcauacucca uaguuauaga auaugagaaa cgaaaaaaaa aaacaguaau aaaaaaagaa     3420

cucauccaaa uaaacauuau ggaaaggguu gcuuuugaaa aagaccaaaa agcguuucua     3480

gaagaaaaag gauauuauuc gccaaaaguu cuaacaaaaa uucccaaaua cacucucuau     3540

gaaugugaaa acgggcgacg aaggauguua gguagugcua augaagcuca gaaagguaau     3600

cagauggugc uaccaaauca ucugaugaca cuuuuauauc acgcaaaaaa uugcgaagca     3660

aaugauggag aaucacuagc cuacauugaa augcaucgug aaauguuugc agaacuuuug     3720

gcauauauuu cagaauucgc aaaacgauau acauuagcca augacagauu ggaaaaaaua     3780

aauauguuuu uugaacaaaa caaaaagggc gauauuaaag ucauugcuaa aucauucgau     3840

aaacuuaaag uauucaacgc auuuggcgca ccucgcgacu uugaauuuuu ugaaacaacg     3900

aucaaacgaa aaagauauua uaacaucaaa gaacuucuaa augccacgau caucuaccaa     3960

agcauaacag gacuguauga agcuagaaaa agacuagaag auuga                     4005


<210>  5283
<211>  4008
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5283
augaaaaacu auacgauagg cuuagauaua ggaacaaaca gcgugggcug ggcuguaaua       60

aaagaugauc uaacacuggu aaggaagaaa aucaaaauau cagguaacac agauaagaaa      120

gaaguaaaga aaaauuuaug gggcagcuuc cuauucgaac aaggagauac ggcacaagau      180

acgagaguca aacgaaucgc aagaagaaga uaugaaagac gacgauuccg aauaagagaa      240

cugcagaaaa ucuuugauaa aucaaugggu gaaguggaca guaacuuuuu ccauagauua      300

gacgaaucau ucuuggugga agaagauaaa gaauauucua aauacccaau cuucucuaau      360

gaaaaagaag acaaaaauua uuaugauaaa uacccgacga ucuaccacuu aagaaaagau      420

uuagccgauu caaaucaaaa agcggaucuc cgacuaauau accuggcauu agcacauaug      480

auaaaauauc gcggacacuu cuuaauugaa ggcgacuuaa aaauggacgg aaucuccauu      540

agugagagcu uucaagaguu cauagacagu uauaacgaag uuugcgccuu agaagaugaa      600

aaucuagaaa uaaucuacaa ugaugaacua uuaacacaaa ucgaaaacau auuuaaacaa      660

gacaucaguc gaucaaagaa acuagaucaa gcuauagcgc uuuuucaagg aguaaaaagg      720

caaagccuau uugguauauu ucuaacacuu aucgucggaa acaaagcaaa cuuccagaaa      780

auuuucaauc uagaagacga uauaaaacuu gaucuaaaag aagaagauua ugacgaaaac      840

uuagaggaac uacuaucaaa cauagaugaa ggauaccgag augucuucuu acaagcuaaa      900

aacguauaca augcaauaga auuaagcaaa auacuaaaaa cugacggaaa agaaacaaaa      960

gcaccguuau cagcacaaau ggucgaacua uacaaccaac accgcgaaga uuuaaagaaa     1020

uauaaagauu auauuaaggc uuaccuaccc gaaaaauacg gagaaacauu uaaagaugcc     1080

acuaaaaaug gauaugcagg uuacauagau gguaaaacau cacaagaaga uuuuuacaaa     1140

uucguaaaag cacaacuaaa aggugaagaa aacggagaau acuuccuaga agcuaucgaa     1200

aaugaaaauu uucuacgcaa acaaagauca uuuuacaacg gaguuauacc auaucaaaua     1260

caucuacaag aacuaacagc ugucuuagac caacaagaaa aacauuauuc cuuucuuaaa     1320

gaaaacagag acaaaaucau cucauuauug acauucagaa ucccauacua cgucgguccu     1380

cuugcaaaag gcgaaucaag auucgccugg cuagaaagau caaauuccga agaaaagaua     1440

aaaccaugga acuucgacaa aaucguagac auagauaaau cagcagaacu cuucauagaa     1500

aaucuuacau cucgagauac auaucuuccg gaugaacccg uacuuccaaa acgcagucuc     1560

auauaucaaa aauucacgau uuuuaacgaa cuaacuaaaa uaucauacau ugacgaaaga     1620

ggaauacuuc aaaacuuuag caguagagaa aagauagcaa uuuuuaacga ccuauucaaa     1680

aacaaaagca aaguaacuaa aaaccaauua guuaaauaca uugaaaauaa agaacagaua     1740

auagccccag aaaucaaagg aauagaagau ucauucaaua guaacuauuc aacauacaua     1800

gacuuaucaa aaauaccaga uaugaagaaa cuuuuggaaa aagaugaaga cgaaauccuc     1860

gaagaaauaa uaaaaauacu aaccauauuc gaagauagaa aaaugcgaaa aagacaacuc     1920

augaaauuua aagacaaacu uucugagaaa gcaauaaauc aacucucgaa gaaacacuau     1980

acaggcuggg gacaauuauc cgagaaauua auuaauggaa uaagggacga acaaucaaau     2040

aaaacgauau uagauuaccu gauagaagau aacggcugcc cuaaaaauau gaaucgaaac     2100

uuuaugcaac uaauuaauga ugacacccua ucauucaaag agaaaaucag aaaagcacaa     2160

gacauaaauc agguaaacga uaucaaagaa auaguaaaag accucccagg aucaccagcc     2220

aucaagaaag gaauauacca aucaauccga auaguggaug aaauaauuag aaaaaugaaa     2280

gaucgcccca aaaauaucgu caucgaaaug gcacgagaaa accaaacuac acaggaaggc     2340

aaaaauaaaa guaaagcaag auuaaagaaa auacaagaag gauuagaaaa ucuagauucu     2400

gugcacguag agaaacaagc ccuagacgaa gaaaugcuua aaucaccaaa auacuacuua     2460

uacugucuac aaaauggaaa agacauauac acaggaaaag accuagacau aggacaacua     2520

caaacauaug auauagacca uauaauucca cguagcuuca uuacagacaa cucauucgau     2580

aacuuaguac uaacuucaag uacaguuaac agaggaaaac uugauaacgu uccgagcccu     2640

gauaucguca ggcaacaaaa aggauucugg aaacaacuac ucagagccgg acuuauguca     2700

caacggaaau ucaauaaccu aacaaaaggc aaacuaaccg acagagacag acaacaguuc     2760

aucaauagac aacuuguaga aacuagacaa aucacaaaac auguagcuaa cuuacuaagc     2820

caucaucuua augaaaagaa agaagucgga gaaauuaaca ucguccuauu aaaaucugca     2880

cuaacgaguc aguuucgaaa gaaauucgau uuuuauaaag ugagagaagu gaaugacuac     2940

caucacgcuc acgacgcaua ucuaaacgga guuauagcau uaaaauuauu agaacuauau     3000

ccauauaugg ccaaagaccu cauauacgga aaauacagcu accauaggaa aauagaagga     3060

gacaaagcaa cacaagccaa auauaaaaug ucgaauauaa ucgagcgauu uucccaagac     3120

cucuuggcca auccagacgg cgaaauagca ugggagaaag acaaggauuu aaauacaauc     3180

agaaagguac uaagcagcaa acaaaucaau auuauaaaga aagcagaaga aggcaaagga     3240

aggcuauuca aagaaacaau aaacucaaga ccaucaaaga aaacugagaa aaggauacca     3300

auuaaaaaca aucucgaucc aaacauauac ggcggauaua uagaagagaa aauggcauau     3360

uacauagcaa uaaauuaccu cgaaaacgga aaaacuaaga aagccauagu aggcauaucc     3420

auuaaagaca agaaagacuu cgaagggcaa acgaccgaau aucucgggaa aauuggauuc     3480

aacaaagcca gcaucauaaa cucauucaaa aauuauacac uuuucgaacu agaaaauggg     3540

ucucgcagaa ugaucguugg cgcuagcaaa gaaaacgacu cgaaaggaga auuacaaaaa     3600

gggaaucaaa uguaccuucc ucaaaaucua uuggaauuug uuuaccaucu aaaacacuac     3660

aacgaagacg aaacaucaca caaauucauu guugaacaca aagccuauuu cgacgaacuc     3720

uuaaacuaua uaguugaguu cgcaaacaaa uacuuagaac uagaaaacuc aauugagaaa     3780

aucaaagacc ucuaucacgg aaaaggucca gacguggaag aaaaagaacu cguugaauca     3840

uuuauaaacc uacuagcuau uacaaaaugc ggacccgcug cggacauaac auuucuugga     3900

gagaaaaucu cacgaaaaag auaucgaucc acaaacuguc uauggggcag cgagguaaua     3960

uuucaaagcc ccacagggcu auacgaaacu agguuaagac ucgaguga                  4008


<210>  5284
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5284
auggacgaca acuaccagcu uaugcaugcu aaaggcaaau acggauaugg cacacgcaua       60

uauuccgaag gucaaacagc ggccgauaga aggggauucc gaacaacacg aagaagacua      120

aaccguagaa aauggcgcuu aagauuacuu caggacuuuu ucgaaccaua uauccuacca      180

caagacgaag cauucuuuau aagacaaaaa caauccaauc uaguaccaca agauacuaag      240

aaacaauauc ccgaaagcgu acucuuugau gauacgucag auaaagauuu uuauacagaa      300

uaucccacaa uauaucaucu aagacaggcu uugaugacag aaaaccgcca auuugacauc      360

agagaagucu acuuagcaau ucaucauaua guaaaauaua gaggacauuu ccuuaauaau      420

gccccaguua aaacauuuaa aaguggaaac cucgaccuag ccauaaaauu caacgaacuc      480

aacgaagagu ucaaaagugu uuacccugau uuagaacuac aauucaaucu ugaccaaauu      540

gacgaaauaa agaaaauacu acuagacuca acaaaaucac gguucgaccg ucaaggagaa      600

gcuguaaaau uaauauacac aguuagcgaa guaaaaacaa uugaaaaacu gcaaaauaaa      660

auaguuaaac aguuccucag ugguauacua ggacuaaaca cacgauucga uguccuaacu      720

aacacagaau cggaugacuc uauguggaaa uuuagccugg acagugaaag cagugaugau      780

gcucucgacg aaauaauagc acaauuagac gauccagcca uacacguugu agaauuagua      840

aaugaaaucc acagcaguau uacauuagca gguauagucc cagaaggaca aggauuauca      900

gaauccaugg ucgaaaaaua ugaagcacac aaagcucacc uucaauuacu aaaagcagua      960

gaaaaacucg uagacaagaa agcagccgac aaauuggaag ucgcuuacga ccaauauaua     1020

aacggcaaag acucaaaaca guucauaaaa gaagacuucu acaaagcggu ccagagugaa     1080

auaaaaggug ucgcaggcga cgaagucgca gaaauaaacc aauuaauaga gcuagaucag     1140

uuuaugccaa aacagagaac gaaagaaaau ggagcaaucc cacaucaacu acaccaacau     1200

gaacuugaug uaaucauaga aaaucaaucc aaauauuacg acuggcuggg agaaaaaguc     1260

ggcaaugaau auaaacuuga uaaacucgua aguuuuagag uaccuuauua uguaggcccc     1320

cuaguagaca cagcaucaaa agaaaagaaa gacgaaacua gauuugcaug gaugguccgu     1380

aaaaauccag agucgacaga agcgauaacc ccuuggaacu uugaagauau aguagacaaa     1440

aaugcaucag ccgaaaacuu uaucaaacga augacaacaa aagauaaaua cuuauuaaac     1500

gaagauguau uaccaaaaug cucucuucua acacaagauu ucgaaguaua caacgaauua     1560

aacaacauac gagugaaugg ugagaaauuu acggcagaac aaaaacaauu aauacacgac     1620

gaauuguuca caaaacaugc aagcguauca auaaaacagc ugaaagacuu uuuaguaagc     1680

gaacuaaugu acguucgaga uaacuuaaaa guagagggau uagcagauga aaagaaacua     1740

auguccucac ucucaacuca guucgacuau cagaaaauaa uaccugacca aauuaaugau     1800

guuaaauauc gagaagauau agaacaaauc auugaauggu caacuauauu ugaagaugcc     1860

gcaauuuuua aaacaaaauu aaaagaaauc gauuggcuaa cagacgaaca acguaauaaa     1920

cucgcgaaca aacguuauag aggaugggga aguuuaucaa aaagacuguu agccuguuua     1980

guagacgaga auggacaaag aaucaucgac auuuuaaggg acagcaguga caacuuuaug     2040

augaucguaa acagacccga guucaaacaa gcuauauucg augaaaacgc aaacuuaacc     2100

gaaacaaauu caaagaaaga uauguucgaa augauagaca acuucuacac uucaccucaa     2160

aauaagaaag caauccguca aguccuacuc guaguucaag acauacaaaa cgcuauggga     2220

ggaguagcac cacagaaaau cuuuaucgag uuugcacgag aagacgaagu aaauggaaga     2280

aggacaaauu caagauauag ucaaauaaga gaaauguauu cuaaaguuac cgaugaugua     2340

auaaacaaaa caguaagagg agaacuugaa gcaaacaaag gaucauuaaa uaaagaaaaa     2400

cucuuccucu acuuucuaca aggaggaauu gaucuauaca gcggcaaaac uaucaauaua     2460

gauaauaucu caucauacga uguagaccau guccuaccac aauccuuuau aaaagaugac     2520

ucaauuaaca acaaagugcu cguaaacgca aaagguaacc gagucaaaaa cgacaaugua     2580

ccaauggagu uauuuggagc gaaaauguau ccaacgugga aacgcauggu agacacuggg     2640

auaaugucga aagcuaaauu uaaaaacuua acgcuaaacc cggauaacgu ugacaaauuc     2700

aaagccgaag gauucaucaa ccgccaacuu guagagacca gacaaauaau aaagcuaguc     2760

gcccagauau uaaacgauaa auuaggcgau gauacaaaaa uaguaucugu aaaagcaaac     2820

uuaacccacg acaugagggu auucuuugau uuccccaaaa accgaaacgu aaacaacuac     2880

caucacgccu ucgacgcgua cuuaucagca uuuguaggaa cauauuuaua caaaagauac     2940

ccuaaauuac aaccauauuu uguauauggc gaauuuaaga aaauggauaa acuuaaaaac     3000

augacguccu ucgacuuccu ccgugacuua aaacacacag acaaaguggu agauaaagac     3060

acaggggaga uaauagcuga uaaacuagcc guuauaacaa acaugaauaa aauauaugau     3120

uucaagaaag ucuuagugac ccacgaagua cacgaaaacu caggggccau guacgaccaa     3180

acccuauacc cugcaaaacu cgauaaauca cucggucgca aaggagcuaa acaacuuauc     3240

ccgaagaaag augauaugcc cacugcacua uauggagguu acaccggcuc aaaagaugcc     3300

uuuaugucga uaguaagacu uaaaaagaaa gaugaaguuu acuauaaagu aauaggaaua     3360

ccaguucgca uagcagacuc uguacaacac ggaaaagagg ucgacgugga agcacuaacc     3420

caguuuauag aaacaaaauu caccaaagaa aaucuaaaua agaaaaccgg ggaaauaaca     3480

cgaaaaguag aaugguuuga acuuguccuu cccaaagucc uauucggcca agccguauuc     3540

gauggagggc aaccauuuau gcuugguagc agcacuuacc cccauaaucu acgagaacuc     3600

guuuuaacaa aagauucagu aaagaaaaua gcugggaaag aaucgaacaa aaguuaucua     3660

gacgcuuacg acgaaauaau ggaggcaaug aacaaauacu ucccacucua ugauacaaac     3720

aaauuccgug aaaaaguaau agagggaaga ucaaaauuca uagauuugcc ugauaaagac     3780

gaguacgaaa acaauaaacu uguacaagua ggcaaaucaa ccauaaucga cagaauguuu     3840

auaggucuac augccaaugc ugcacgaacc gaucuuaaaa auauagguau aacaacccca     3900

uucggcuuca ugcaacaagc cucuggaaua aaauuaacaa aagauacagu aguuuuauuu     3960

caaucaagua ccggcuuguu ugaacggaaa guagcauuaa aagauuuaug a              4011


<210>  5285
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5285
augacaaaag acuacacaau uggacuugau aucggaacaa acucaguagg augggcaguc       60

cuaacagacg acuaccaauu aaugaaaaga aaaaugucag ugcauggaaa uacagagaaa      120

aagaaaauua agaaaaauuu cuggggagca aggcuauucg acgaaggaca aacagccgaa      180

uuucgaagaa ccaaacgcac uaaccgacga cgccuagcua ggagaaaaua ccguuuaucc      240

aaacuacaag accucuucgc ggaagaauua uguaaacaag augacugcuu cuuuguaaga      300

cuugaagaau ccuuucuagu uccagaggaa aaacaauaca aaccagcuag uaucuuuccc      360

acauuagagg aggaaaaaga auacuaccaa aaauacccca cgauauauca uuugagacaa      420

aagcuaguag acagcacaga aaaagaagau cuucgacuug ucuaccuagc auuagcccau      480

cuccucaaau accgaggcca cuuucuguuc gaagguuacc uugacacaga aaacacauca      540

auagaagaau cuuuuagggu auuccucgaa caauauggga aacaauccga ucaaccacuc      600

auaguacacc aaccaguccu aacaauuuua acagauaaac uaaguaaaac gaagaaaguu      660

gaagaaaucc uaaaauauua uccgacagag aaaauaaacu cuuucuuugc acaauguuua      720

aaauuaauag uagggaauca agcaaauuuc aaacgaauau ucgaccuuga agcagaagua      780

aaacuacaau uuuccaaaga aacauacgaa gaagaccuug aaagucuauu agagaaaaua      840

ggcgaugaau acuuagacau auucuugcaa gcuaaaaagg uccaugacgc aauacuccuu      900

agcgaaauca ucaguucaac uguaaaacau acucaagcaa aacucucaag cgguauggua      960

gaacgauaug aaagacauaa agccgaccuu gccaaauuca aacaauuugu aaaagaaaau     1020

guaccgcaaa aagcuacagu cuucuuuaaa gacacaacca aaaauggcua cgcuggguac     1080

aucaaaggca aaacuacaca agaagaguuc uacaaauucg ucaagaaaga acucagcggu     1140

guagucggau cagaaccauu cuuagagaaa auagaucaag aaacauuccu gcucaaacaa     1200

agaacauaca caaauggagu aauuccacau caagugcauu uaaucgaacu uaaagccaua     1260

auagaccaac aaaaacaaca uuacccauuu cuagaagaag cgggacccaa aauaaucgcg     1320

cuuuucaaau uucgaauacc auacuaugug ggacccuuag ccaaagaaca agaagcgagc     1380

ucauucgccu ggauagaaag aaaaaccgcu gagaaaauac auccauggaa cuucucggaa     1440

gucguugaca ucgagaaaag ugcaaugaga uucauacaac guaugacaaa acaagacacu     1500

uaucugccaa cagaaaaagu acuccccaaa aauucauuac uauaccaaaa auacaugauc     1560

uucaaugaac uuacaaaagu aaguuacaaa gacgaaagag gaguaaaaca auauuucucu     1620

ggcgaugaga aacaacaaau auuuaaacaa cucuuucaaa aagaaagagg uaaaauaaca     1680

gucaagaaac uacaaaacuu ucuguauaca cacuaucaca ucgaaaacgc ccacauauuu     1740

gguauagaaa aagcauucaa cgccucauac ucaacauacc acgauuuuau gaaauuagcc     1800

aaaacgaacc aaaaaucaau gcaagagugg cuagaacaac ccgaaaugga acccauuuuc     1860

gaagauauag uaaaaauccu aaccauuuuc gaagacagac aaaugauuaa acaccaacuc     1920

uccaaauauc aagaaguauu cggcgaaaaa uuacucaaag aguucgcccg aaaacacuau     1980

acugguuggg gacgauuuag ugcaaaauua auacacggca uucgugaucg aaaaacaaau     2040

aaaacuauuc ucgacuauuu aauaaaugau gacgauguac cagcaaauag aaaccgaaac     2100

cuaaugcaac uaauaaacga ugaacacuua ucauuuaaag aagaaauagc aaaagccacu     2160

gucuucucaa aacacaagag cuuaguagac guaauccaag aucuaccagg cuccccagca     2220

auuaagaaag gaauauggca aaguuuaaaa auuguagaag aauuaauugc cauaaucggu     2280

uacaaaccca aaaacaucgu cauagaaaug gcaagggaaa aucagaaaac gcacagaaca     2340

aaaccacgau ugaaagccuu agaaaauggc uuaaaacaaa uaggcucaac acuacuaaaa     2400

gaacaaccca cagacaauaa agcucuccag aaagaacgcu uauaccuaua cuaucuacaa     2460

aauggacgug auauauauac aggagaacca uuggauauag aaaaucuuca ucaauaugag     2520

guagaccaua uaaucccuag aucauuuaua guggacaaua guaucgauaa uaaggugcua     2580

guagcgagua agcaaaauca aaagaaacgu gacgacguac ccaauaaaca gauagucaac     2640

gaacaaagaa uauucuggaa ccaacugaaa gaagcuaaau uaauuagucc caagaaauau     2700

gcguaccuua cuaaaauaga acuaacaccc gaagauaaag cccgauuuau ucaaagacaa     2760

cugguugaaa caagacaaau aacaaaacau gucgcuaaca uacuacauca aucauuucac     2820

caggaagaag aaggcacaga uugugacgga guacaaauaa uaacuuuaaa agcaacucua     2880

accucacagu ucagacaaac auuuggucuu uacaagguaa gggaaauaaa uccacaccau     2940

cacgcacaug acgcauaucu aaacgguuuu auagcaaacg uauuacuuaa acgauaucca     3000

aaauuagcac cagaauuugu auacgggaaa uauguuaaau acagccuggc ucgcgaaaau     3060

aaagcaaccg cgaaaaagga guucuacuca aacauacuga aauuccuaga aucugacgaa     3120

ccauuuugug acgaaaacgg ugaaauauau ugggaaaaau ccuaucaucu uccgcggaua     3180

aagaaaguac uguccucuca ccaaguuaau guaguaaaga aaguagaaca acaaaaagga     3240

ggcuucuaca aagaaacagu aaauucaaaa gaaaaaccag auaaauugau cgaacgaaag     3300

aauaacuggg acgucaccaa auacggaggg uuugguucac caguaauugc auaugcaauc     3360

gcauucgucu augccaaagg aaaaacacag aagaaaacac gagcaauuga aggcaucaca     3420

auaauggaac aagcagcauu cgaaaaagau ccaacaacau uccucaaaga uaaaggauuc     3480

ccacaaguaa cugaguucau aaaauuaccc aaauauacgc uauucgaauu ugauaauggc     3540

agacgacgau uuuuagccag ucacaaagaa ucacaaaaag gaaauccauu cauacuauca     3600

gaucaauuag ucacccuacu cuaucaugcg caacauuacg acaaaauuac cuaccaagaa     3660

uccuucgauu acgucaauac acaccuaagc gacuuuagug caauacuaac cgaagucuua     3720

gcuuucgcag aaaaauauac acucgcagac aaaaacaucg aacggauuca agaacucuac     3780

gaagaaaaca aauacggaga aauaucgaug auagcacaga gcuuccuaca acuccuucaa     3840

uuuaaugcaa uaggagcucc agcagacuuu aaauuuuucg gaguaacaau accaagaaaa     3900

agauacacau cuuuaaccga aauaugggac gcaacaauaa uauaccaaag cgucacagga     3960

uuguaugaaa cacgaauaag aaugggugac cuaugggccg gagagcaaug a              4011


<210>  5286
<211>  4011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5286
augaccaaag acuacacaau agggcuagau aucggaacaa auagcguugg augggcagug       60

cuaacagacg auuaccaacu uaugaaaaga aaaaugagcg uccauggaaa uaccgaaaaa      120

aagaaaauua agaaaaacuu uuggggugca agauuauucg acgagggaca aacagcagaa      180

uucagaagaa caaaaagaac caacagacgc cgccuugcac gacgaaaaua uaggcuauca      240

aagcuacaag accuauucgc agaagaauua uguaaacaag acgacugcuu uuuuguuaga      300

cuggaagaaa gcuuccuugu accagaggaa aaacaauaua aaccugcgag uaucuuuccc      360

acuuuagaag aagaaaaaga auacuaucag aaauacccua caaucuauca ucuaagacaa      420

aaacuagucg acucuaccga gaaagaagac cuucgacuag uauaucuagc acuugcacac      480

uuauuaaaau accgaggaca uuuuuuauuu gaaggcgacu uagauacaga gaauacguca      540

aucgaagaau cuuuuagagu uuuuuuagaa caauauucaa aacaaucuga ccaaccgcua      600

auugugcacc aacccguccu aacaauauua acagacaaac uauccaaaac uaagaaggua      660

gaagaaauau uaaaauauua cccgacagaa aagauaaacu cauucuuugc gcaaugcuua      720

aagcuaauag uaggaaacca ggcaaacuuu aaacguauau ucgaccuuga agcagaaguu      780

aaauuacaau ucucuaaaga aacauaugaa gaagauuuag aaucacuucu ggaaaaaaua      840

ggagacgaau accuagacau auuuuuacaa gccaaaaagg uacacgacgc cauauuacua      900

ucagaaauaa uaucuucuac aguaaaacac accaaagcua aauuauccuc uggcauggua      960

gagcgauacg aaagacacaa agccgaccua gccaaauuca aacaauuugu caaggaaaau     1020

guaccucaaa aggcuaccgu guucuucaaa gauacgacga agaacggcua cgcaggguac     1080

auuaagggaa aaacgacaca agaggaauuc uauaaauucg ucaagaaaga gcuauccggg     1140

guuguaggua gcgaacccuu ccuagaaaaa aucgaucaag aaaccuuccu gcuaaaacaa     1200

cgaacauaua caaacggugu cauaccacac cagguucacu uaauugaacu uaaagcaauc     1260

auagaccagc aaaaacaaca cuacccauuu cuagaagagg cagguccuaa aauuauugca     1320

cuauucaaau uucgaauucc auauuauguu gggccucuag ccaaagaaca ggaggcauca     1380

uccuuugccu ggauagaaag gaagaccgcu gaaaaaauca auccauggaa cuuuucagaa     1440

guagucgaua uugaaaaauc cgcaaugcgc uucauucaac gaaugacaaa acaagauaca     1500

uaucucccaa cagaaaaggu guuaccaaag aacagucuuc uauaccaaaa auauaugauu     1560

uucaacgaac uaacaaaagu aucuuauaaa gacgaaaggg gcgucaagca guacuucuca     1620

ggagacgaaa aacaacagau uuucaaacag uuauuucaga aagaaagagg aaaaauaacc     1680

gucaaaaaau uacaaaacuu uuuauacaca cauuaccaca uagaaaacgc acaaauauuu     1740

gguaucgaga aagcauucaa cgcaaguuau aguaccuauc acgacuucau gaaacuagcc     1800

aaaacgaacc aaaaagcuau gcaagagugg cuugaacaac cagagaugga accaaucuuc     1860

gaagacauag uaaaaauccu aacuauauuu gaagaucgac aaaugauuaa acaucagcuu     1920

uccaaguacc aagaagucuu cggcgaaaaa cuucuaaaag aauucgcacg caaacacuac     1980

acaggcuggg gaagauucuc cgcaaaacuu auucacggga uuagagacag aaagacaaac     2040

aaaacaauau uggauuaucu aaucaacgac gacgauguac cagccaauag aaaccggaau     2100

cucaugcaau uaauaaacga cgaacaucua ucuuucaaag aagaaauagc aaaagcaacc     2160

guauucagua aacauaaauc acuagucgac guaauccaag accuaccagg caguccugca     2220

aucaaaaaag gaaucuggca aucacuaaaa auagucgaag aacuaaucgc caucauaggu     2280

uauaaaccaa aaaauauagu cauagagaug gcaagagaaa accaaaaaac acaucgcaca     2340

agcccccguu uaaaagcauu agaaaacgga uuaaaacaaa uuggaagcac ucuacuaaaa     2400

gaacaaccca cagauaauaa agcccuucaa aaagaaagac uauaccuaua uuaccuucaa     2460

aacggccgug auauguauac aggagaaccc uuagagauag aaaaucuaca ccaauacgaa     2520

guugaucaua ucauuccaag aucauucaua guagauaauu cuauugacaa caaagugcuc     2580

guagcuagca aacaaaauca aaaaaaacgu gacgacguac caaaaaagca aauuguuaac     2640

gagcaacgaa uauucuggaa ucaauugaag gaagccaaac ucauuucacc uaaaaaauac     2700

gccuaccuaa ccaaaauaga auuaacaccc gaagacaaag cacgauuuau acaacgacaa     2760

cugguagaaa ccaggcaaau caccaaacau guagcuaaca uauuacacca aaguuuuaac     2820

caagaagaag aaggcacaga cugcgacgga guacaaauaa uaacacuuaa ggcuaccuua     2880

acaucucaau uucgccaaac uuuuggauua uauaaaguca gagaaaucaa cccccaccau     2940

cacgcgcaug acgcauaccu aaacggauuc aucgccaacg uucuacuaaa acgauaccca     3000

aagcuagcac cagaauucgu cuaugguaaa uacguuaaau auuccuuagc aagagaaaau     3060

aaagcgacag caaaaaagga auuuuauucc aacauauuaa aauuccuaga auccgacgaa     3120

ccauuuugcg augaaaaugg agaaaucuau ugggaaaagu cacaccacuu accaaggaua     3180

aaaaaagucu uaucgaguca ccaaguaaac guagugaaga aaguagaaca acaaaaaggu     3240

ggauucuaca aagagaccgu caauucaaaa gaaaaaccag acaaacuuau agagagaaaa     3300

aauaauuggg acguaaccaa auacggagga uucggaucuc ccguaauagc guacgcaaua     3360

gcauucguau augcaaaagg aaaaacucaa aaaaaaacgc gagcuaucga aggaauaaca     3420

auaauggaac aagcagcauu cgagaaagau ccaacaacau uccuaaaaga aaaaggauuc     3480

ccacaaguga ccgaauucau uaaacuccca aaauauacau uauuugaauu ugacaauggc     3540

agacgaagau uccuggccuc ucauaaggaa ucacaaaaag gaaaccccuu cauauuaagu     3600

gaccaauuag uaacacuacu guaucacgcc caacauuaug auaaaaucac auaccaagaa     3660

agcuuugacu acguuaacac acaccuuagc gacuuuagcg ccauacugac agaaguacua     3720

gcauuugcag aaaaauauac ccucgcagau aaaaacauag agcguauaca ggaacucuac     3780

gaagaaaaca aauacgggga aacaaguaug auagcacaau cauuccucca auuacuucaa     3840

uucaaugcaa uaggagcccc ugcagacuuu aaauucuucg gaguaacaau cccuagaaaa     3900

agauacacgu cccuaaccga gauaugggac gcuacuauua uauaccaauc cguaacaggc     3960

cucuacgaaa cucgaauaag aaugggagau uugugggccg gcgaacagug a              4011


<210>  5287
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5287
augaaaaaca ugaagaaacc uuauaccauu ggauuagaua uaggcacuaa cagugucggu       60

ugggcagugc ugacagacca auacgaucug guaaaacgaa aaaugaaaau ugcaggagau      120

uccgagaaaa agcaaaucaa aaagaauuuc uggggaguaa gacucuuuga ugaagggcaa      180

acggcagcag acaggagaau ggcgagaaca gcccggcgaa gaauagaaag aagaagaaau      240

agaauuaguu accuucaagg aaucuuugca gaagaaaugu caaagacuga cgcgaacuuu      300

uucugcagau uaagcgauuc auucuaugua gauaaugaga aaagaaauuc cagacaucca      360

uucuucgcaa caauagaaga agaaguugaa uaucacaaaa auuacccaac cauauaucau      420

cuaagagaag aacucguaaa uaguuccgaa aaagcggauc ugcgacuugu cuacuuagcg      480

uuggcacaca uaaucaaaua cagagguaau uuccuaauug aaggggcauu agacacacaa      540

aauacaagug uagauggaau cuauaaacaa uuuauucaaa cauauaauca aguauuugca      600

aguggaauug aagacggauc auuaaagaaa uuagaagaca auaaagaugu cgccaaaaua      660

uugguggaaa aagugacuag aaaggagaaa uuagaaagaa uauuaaaacu auacccggga      720

gaaaaaucag cggggauguu cgcucaguuu auaucauuaa ucguuggauc caaaggaaau      780

uuccaaaaac cuuucgacuu aauagagaaa agcgacauag aaugcgcgaa agacagcuac      840

gaagaagacc ucgaaagucu ccuagcccua aucggagaug aauaugcaga acuauuuguu      900

gcugcuaaga acgcauauuc ugcagucguc uugagcucaa uaauaaccgu agcagaaaca      960

gaaacaaaug caaaacuuuc ugcuagcaug auagaacguu uugauaccca cgaagaagac     1020

cuuggagaau uaaaagcauu uauuaaacua cauuuaccaa aacacuauga agaaauauuc     1080

agcaacacag aaaaacaugg cuacgcggga uacauagacg guaagacaaa acaagcagac     1140

uucuacaaau acaugaaaau gacacuugaa aacaucgaag gagcugacua cuuuauagca     1200

aaaauugaaa aagaaaacuu ucuaagaaaa caacgaaccu ucgacaacgg agcaauccca     1260

caucaauuac aucucgagga acuugaagca auucuccacc aacaagcaaa auacuacccc     1320

uucuuaaaag aaaacuauga caaaauuaaa ucccuaguga cauuuagaau accauacuuc     1380

guaggcccgc ucgcaaaugg gcagucagag uuugccuggc uaacacgcaa ggcugauggg     1440

gaaaucagac cauggaacau agaagagaaa guugacuucg gaaaaucagc aguagauuuc     1500

auagagaaaa ugacuaacaa ggauacuuau cuaccgaagg aaaauguuuu acccaaacau     1560

agcuuaugcu accaaaaaua ccuaguauac aacgaauuaa ccaaaguaag auacaucaac     1620

gaucagggaa aaacauccua uuucucuggu caagaaaaag aacagaucuu uaaugacuug     1680

uuuaagcaga aaagaaaagu caaaaagaaa gacuuagaau uauuuuuacg aaacaugucc     1740

cauguggaaa guccaaccau agaagguuua gaagauucau ucaauucaag uuauucuacg     1800

uaccaugacc uacuaaaagu cgguauaaaa caagagauuc uugacaaucc cguaaacacc     1860

gagaugcugg aaaacauagu caaaauacua acuguauuug aagauaaacg aaugaucaaa     1920

gaacaguugc aacaauucuc agacguccuc gauggagucg uccucaagaa acuagaaaga     1980

agacacuaua caggaugggg acgucuuagc gcaaaauuau uaaugggaau aagagauaaa     2040

caaucacauc uuaccauacu agauuaccua augaaugaug acggauuaaa ccgaaacuua     2100

augcaauuaa uaaaugacag uaacuuaagu uucaagucaa ucauugaaaa agaacaaguc     2160

acaaccgccg auaaagauau acagagcaua gucgcagauc uagccggaag cccugccaua     2220

aagaaaggga uucuacaaag uuuaaaaaua gucgaugaac uugucuccgu aaugggauau     2280

ccaccacaaa ccauaguugu agaaauggcc cgcgaaaauc aaacaacagg aaaaggcaaa     2340

aauaauagcc ggccuagaua caaaucacua gaaaaagcca ucaaagaauu ugguagucaa     2400

auacuaaaag agcaccccac cgacaaucaa gaauuaagaa auaacaggcu uuaucucuac     2460

uauuuacaaa auggaaaaga cauguacaca ggacaagauu uagacauuca caaccuaucu     2520

aacuacgaua uagaccacau cguuccgcaa aguuuuauaa cagauaacag cauugauaac     2580

cuaguacuaa cgucaucagc cggaaaucgc gaaaaaggag augacguacc accccuugaa     2640

aucguaagaa agagaaaagu auuuugggaa aaacuauauc aaggaaacuu aaugaguaaa     2700

agaaaauuug acuaucuaac caaggcugaa agaggcggau uaaccgaagc agacaaagcc     2760

agauucaucc auagacaauu gguagagaca cgucaaauaa ccaaaaaugu agcaaacaua     2820

cuucaucaac gauuuaauua ugagaaagau gaucacggaa auacaaugaa gcaggucaga     2880

auuguaaccc uaaaaagugc acucguauca caauuccgca aacaauuuca acuguacaaa     2940

gucagagaug ucaaugacua ccaucacgca cacgaugcau aucuaaacgg cguaguugca     3000

aauacucuau uaaaaguaua cccacaacuu gaaccagagu ucguauaugg ggauuaccau     3060

caauucgauu gguucaaagc aaacaaagca accgcaaaga aacaguucua uacaaauauu     3120

augcuuuucu uugcacaaaa agaucgaauc auagacgaga acggagaaau acuaugggac     3180

aagaaauacc uggauacagu uaagaaaguu auguccuaua gacaaaugaa caucgugaag     3240

aaaacugaaa uacaaaaagg ugaguuuuca aaagcaacga uaaaaccaaa agguaacucg     3300

uccaaauuaa uaagcagaaa aacaaauugg gacccaauga aauauggagg acucgauucg     3360

ccaaacaugg cauaugcagu cgucauagaa uacgcaaaag guaaaaacaa acuuguuuuu     3420

gaaaagaaaa uaauaagagu gacaauaaug gaaaggaaag ccuuugaaaa agaugaaaaa     3480

gccuuccuag aagaacaagg uuauagacaa ccuaaagugc uagcaaaauu accgaaauac     3540

acauuauacg aaugcgaaga aggaagaaga cgcaugcucg ccuccgcaaa cgaagcacaa     3600

aaagguaauc aacaaguuuu accaaaccau uuaguaacac uauuacauca uguagcaaau     3660

ugugaagucu cagacggcaa gucauuagau uacauagaaa gcaaccgaga aauguucgca     3720

gaauugcuag cacacguuuc ugaguucgca aaaagauaua cguuagcaga agcuaaucuc     3780

aacaaaauaa accaauuauu cgaacaaaac aaagaagggg acauuaaagc aaucgcacaa     3840

uccuuuguag acuuaauggc auucaaugca augggagcac cggcaucauu uaaauuuuuc     3900

gaaaccacaa uagaaagaaa aagguauaac aaccucaaag aacuucuuaa uucaacuauu     3960

auauaccaaa gcauaacagg ucuuuacgag ucuaggaaaa gauuagacga uuga           4014


<210>  5288
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5288
augaaaaaau ucgaaaacua uuacuuaggc cuugauauug guacaagcuc aaucgggugg       60

gcgguaacga acucucagua cgauauuuua aaauucaacg gcaaauacau guggggaaca      120

cgauuguucc cagaagcaaa uaccgcucaa gagagaagaa uacaucguuc aagucgaaga      180

agacucaaaa gacggaaaga gagaauccaa auacuucaaa ugcuuuuuga uaaagagauc      240

gccaagauug auagcggauu uuuucaacga cuuaaggacu caaaguauua caaggaagau      300

aaaacugaaa aacaaaccaa uuccauuuuu caugacaaag auuauucaga uaaagaauac      360

caucaagacu uucccacaau auaucaucuc agaaaauucc ucuuagaagg aaauaaacca      420

aaagauauaa gauucgugua ccuagcgcua caccacauuu ugacacauag aggacacuuu      480

uuauuuccug acauggaagu gaguaacguc acagaauucu caaauauuuu uagugaacua      540

aaacaguauu uauaugacga aauggaccuc gauuuugaau ggaaaaaugg aaaauuaaau      600

gaaguugaaa acauauuaaa agaccgaaac uuagcuaaaa gcgaaaaaga aaaaaaguua      660

ugcggaauaa uagaguuaga aggaaaaaua gacaaucaaa gaaaggccau gauuggaaug      720

augugcgggu gcaaaaaaaa auucucagac cuguuccaaa acgaaaacua ccgcgaacuc      780

gaaauauccu cuuucucauu cgcagauagc ucauacgaag aaaaacgaga cgaacuugaa      840

ggaauacucg ccgaaaacau ccuuugucua gacuaccuaa agacaauaua cgacugggga      900

aaacuaucag acauucuaca aggcgaagag ucaaucuccg ucgcaaaagu gaaaucauac      960

gaggaacauc aacucgaccu aaaauauuua aaaaauaucu uaaaauugua cagucauaaa     1020

gaaaaggaaa acguauuccg gaaaaaagaa ggaaaauauc caaaauacau aagugggaaa     1080

cucucacaag aagaauuuaa caaaaguaua aaaaaaauac uagaggaaau uaaaagcgug     1140

aagcaagaag acaggaaagu auuugacacg uuguuaaaaa gagccucaaa caaucuguua     1200

ugccccaaac agguaaucaa agaaaauggg gucauacccu accaaauuca caaauuugaa     1260

uuagaaaaaa uucuccgcaa uauggccgau uucuucccaa ugcuaaaaga aaaaaaggac     1320

ggaaaaacac uaucggaaaa aauaauaagc guuuucacau ucaggauccc auacuacgua     1380

ggacccuuaa aucagaacuc ugacagagcc ugguuaguaa aaaacaaaga ugaaaagauu     1440

uacccaugga auuucgaaga aauaguaaac cuagaagagu cugccgagaa auucauucaa     1500

aacuuaacua auaaauguac auaccuaguu cuggaagacg uacuaccaaa aucaucaauu     1560

uuauauagua aauuuauggu acuaaaugaa cucaacaacc uaaaaauaga cggagaagcc     1620

auuagugucg aucugaaaca aaaaauuuac cuuaacuuau uccaaaaaua uaaaaaaguu     1680

acucucaaaa aacuaaaagg auaucuaaag ucagaaaaca uauuaauaga uacgaguacu     1740

caaauaacgg gcaucgacgg ugauuucaaa aguucccugg ggucguaucu agacuuuuac     1800

aacauauuag gagacaaagu aaaaacugau uucggcaaaa aacuaauuga aaauugcauu     1860

uuauggauaa cuuuauauac aggagaaaaa aaacuucuaa aaaacaaaau aauagcaaau     1920

uauaaaggag aacuuaguga agaagaaaua aagaaaauag uaaaucucaa guacaaagau     1980

uggggaagac ucagcuacgc auuucuagaa gagauccaau cagcgucacu agaaacugga     2040

gaacuacgaa acaucauaca aaugaugugg gagacgaaca auaacuugau ggaauuauua     2100

ucaagcaacu accaauuucu aucugaaaua gaaaagagga acagcguagu agccaucggc     2160

aaggaauuca acuaugaaac gauauuaggc gauucauacg ccucaccauc aguaaagaga     2220

augauauggc aaucauuaag cguugucgau gaaaucaaaa aaauaaugaa aaaagcacca     2280

aagaaaauau uuauagaaau ggcacgccaa gaagacauga aaaaggaaag aaaagagucc     2340

agaaaaagca ccuucuuaac acuauacaaa ucaauuaaag aagaaggaag ggacuggauc     2400

aaggaaaucg aaaauugguc agauagcgaa uuuagaagca aaaaacucua cuuauauuac     2460

acgcaaaugg gaaaauguau guauacuggc gaaaaaauaa gccuagacca auuauuuaau     2520

aaaaauauau acgauauaga ucacauuuac ccacgaucca aaaccaaaga cgauucaauc     2580

gaaaacauug ucuuaguaaa aagaaacaua aacgcaaaaa aaaccgauga auacccacuc     2640

gaaagaaaca uacaacaaaa acaacaugau uuuuggaaaa ugcuacacag uaaaaagcua     2700

aucggugaca aaaaauacga acggcuuacg cgaacaacag aauucacuga cgaagaacuc     2760

uccgauuuca uagcaagaca auuagucgaa accagacaaa gcacaaaaau aguagcagau     2820

auuuuaaaaa accuguuccc agaaacaaaa aucguauacg uaaaggcgaa ccucaccucc     2880

gacuucagaa agaacuuuaa aauacuuaaa agcagagaca uaaaugacua ccaucacgcu     2940

cacgacgcau auuugaacau aguaacugga aacgucuaca auauaaaauu cacggauaac     3000

ccaagaaacu ucauaaaaga uaaaaaauua gaaggcaaaa aauacaauuu aaaagucgaa     3060

aaaauauucg aauaucgaaa agaaaaugaa aguuucugga acccuaaaac aaugguaaca     3120

aaaaucgaaa auuacaucuu caaaaagcga ccacaauuca ccagguacuc guucgaacaa     3180

caugguggau ucuuugacca gaauauagua gacaaagaaa guugcaaaaa aggcacggga     3240

uacauuccca uaaaaucauc aaacgccaua uuauacaaua ccaguaaaua cgguggauau     3300

ggaucaguaa ccgggaccua uuucuuccuc guagagcaua caaugaaagg aaagagaauc     3360

cgaaccaucg aaaugaugcc ucuuuaucua ucgaaaaaaa uacacagcaa ggaagaauua     3420

gaaaaauacu guaaagaaaa acuagaauua cagaaaccgu caguuagauu agcaaggaua     3480

aaauauaacu cauuacuuaa aauaaauggc uuuccuuacc auauaacugg uaaaacuaac     3540

gauaguuauu ggauaaugag cgcaauacaa cuacuauuau caaaaaauua cuacgaauac     3600

cuaagaaaac ucuauauauu cugcaaagag gagcgacuug aggaggaaau cgucggagaa     3660

aagaacauca aacucuacac uugcauacuu gaaaaauuag agaaaagcau auacagccau     3720

aaacucuuaa acauuaauua caauggaaaa aguaaaaacu uaaaagacau acucgaaagc     3780

gaaaaagaaa acuuucuacu ucucagcgaa aagaaacaag cacaaauucu aaucgaaaua     3840

uucacauuau uagaaucaaa uaacuucgga gccaacuuag aaucauucgg augcggaaaa     3900

aaaugcggaa uaaccaaaau aaacaaaaac auugauaaau uagaagaagu ccuauuaauc     3960

aaucaaucac cgaccggacu uuuugaaaac ucaauagacc ucaaaaaagu cuga           4014


<210>  5289
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5289
augaaaaagg auuauguaau aggucuagau auaggaacga acagcguagg augggccguu       60

augacagaag acuaccaauu aguaaagaag aaaaugccaa uuuacgggaa cacagagaaa      120

aagaaaauaa agaaaaacuu cuggggagua agauuauucg aagaaggcca uacagcagaa      180

gacagacgcu uaaaacggac agcaagaagg agaauaagua gacgaagaaa ucgucuacgu      240

uaccuacaag ccuucuuuga agaagccaug acagaucuag augaaaacuu uuucgccaga      300

cuccaagaau cauuccucgu accagaggac aagaaauggc auagacaucc aauuuuugcu      360

aaauuagaag acgaaguagc auaucacgaa acauauccua ccauauauca cuuaaggaag      420

aaacuggcag auagcaguga acaagcagac cuacgacuaa ucuaucuagc acucgcccac      480

aucguaaaau accgaggaca uuuccuuaua gaaggcaaau ugagcacuga aaacauauca      540

guuaaagaac aguuccaaca auucaugaua aucuauaacc aaacuuuugu aaacggcgaa      600

uccagauuag uuucugcacc ccucccagag ucagucuuaa ucgaagaaga acuaacagaa      660

aaagcgucaa gaaccaagaa auccgaaaaa guauuacaac aauuuccaca agaaaaagcc      720

aacgggcuau uuggacaauu ccuaaaauua augguaggaa auaaagcaga uuucaagaaa      780

guauucgguu uagaagagga agccaaaauu acguaugcca gcgaauccua ugaagaagac      840

cuagaaggca uucuagcaaa agugggagau gaauacucug auguauuccu agccgcgaaa      900

aauguuuacg augcaguuga acuuucaacc aucuuggcag acagcgauaa gaaaucacac      960

gcaaaauuau cuucaaguau gauaguaagg uuuacagaac aucaggagga ucucaagaaa     1020

uucaaaagau uuaucagaga aaacugucca gaugaauacg auaaucuguu uaaaaacgaa     1080

caaaaagaug gauacgcagg auauauugca cacgcaggua aagugucaca gcuuaaauuu     1140

uaucaauaug ucaagaaaau aauucaagau auagcuggcg cagaauacuu ccuagagaaa     1200

auagcccaag aaaacuuuuu acguaaacag agaacauucg acaacggugu aauaccccac     1260

caaauucacu uagccgaacu ucaagcuauu auacauagac aagcagcuua uuacccguuc     1320

cuaaaagaaa accaagagaa aauagaacaa cucguaacgu uucgaauacc auacuacguu     1380

ggaccccucu cuaaaggaga ugcaaguaca uucgccuggc uaaaaaggca aucagaagaa     1440

ccuauucgac cauggaaucu acaagaaaca gucgauuuag aucaaucagc aacagccuuc     1500

aucgaacgaa ugacaaauuu ugauacuuac uugccuagcg aaaaaguauu accaaaacau     1560

uccuuauuau acgaaaaauu cauggucuuc aaugaacuca ccaaaauuuc cuauaccgac     1620

gaccggggaa uaaaagccaa cuuuagugga aaagaaaaag agaaaauauu cgauuaccuu     1680

uucaaaacaa gacgaaaagu caaaaagaaa gauauaauac aauucuaccg aaacgaauac     1740

aauaccgaaa uaguaacucu aucaggauug gaagaagacc aauucaacgc aucauuuucu     1800

acuuaccaag accuacuaaa augcggguug acuagagccg aauuagauca uccugacaau     1860

gcagaaaaau uagaagacau aauaaaaaua cuuacuauau ucgaagauag gcaaagaauc     1920

agaacccaac uaucgaccuu uaaaggacaa uucucagcug aaguauuaaa gaaauuagaa     1980

cgaaaacacu auacaggaug gggaaggcuu agcaaaaagc uaauuaaugg uaucuaugac     2040

aaagaaagug gaaaaacaau acuaggauau cuaauuaaag augacggugu uuccaaacau     2100

uacaacagaa acuuuaugca auuaauaaac gauagucaac ucagcuuuaa aaacgcaauc     2160

caaaaagcac aaucaucaga acaugaagaa acucuaucag aaacaguuaa cgaacuagca     2220

ggaucgcccg ccaucaagaa aggaauauau caaagccuua aaauuguuga cgaacucgua     2280

gcaaucaugg gcuaugcacc aaagcgaaua guaguugaaa uggcaagaga gaaccagaca     2340

acaucaacag gaaaacguag guccauccaa cgcuuaaaaa uaguugaaaa agcaauggca     2400

gaaauaggua guaaccuacu uaaagaacag ccaaccacua augaacaacu aagagauacu     2460

agauuauucc uauacuacau gcaaaacggc aaggauaugu acacuggcga cgaacucuca     2520

cuccauagau uaucccacua ugauaucgac cacaucauac cucaaucauu uaugaaagau     2580

gacucacuag auaaucuugu ucuaguaggu agcaccgaaa accguggaaa aagugacgau     2640

guaccaucaa aagaaguugu aaaggacaug aaggccuauu gggaaaaacu auacgcagcc     2700

ggguuaaucu cucaaagaaa auuccaaaga cuaaccaaag gagaacaagg cggauuaacu     2760

uuagaagaua aggcacacuu cauucaaagg caacucgucg aaacaagaca aauaaccaaa     2820

aacgucgcag gaaucuuaga ucaaagguau aacgcuaacu ccaaagaaaa gaaaguccaa     2880

auaauaacac uaaaagcgag ccuaacauca caauuuagau caauauucgg cuuauacaaa     2940

guaagagagg ucaaugacua ucaccacgga caagaugccu aucucaauug cguuguagca     3000

accacccuau ugaaaguaua cccgaaccua gcucccgagu ucgucuaugg agaauaccca     3060

aaauuucaaa cauucaaaga aaacaaagca acagcaaaag ccauaaucua cacuaaucuc     3120

uuaagauuuu ucacagaaga cgagcccagg uuuaccaaag acggagaaau acucugguca     3180

aacagcuacc ucaaaacaau caagaaagaa uuaaacuacc aucaaaugaa uauaguaaag     3240

aaaguugaag uacaaaaagg agguuucucc aaagaaucaa uaaaaccuaa aggaccauca     3300

aauaaacuaa uacccgugaa aaauggauua gacccacaaa aauacggagg auuugauuca     3360

ccaauaguug cauauacagu auuauucacu cacgaaaaag gaaagaaacc uuugauaaaa     3420

caagaaaucc uagguaucac cauaauggag aaaacaaggu ucgaacaaaa uccuauauua     3480

uuuuuggaag aaaaagguuu cuuaagacca agaguauuaa ugaagcugcc aaaauacacu     3540

cuauaugagu ucccagaagg acguagaagg cuguuggcaa gugcaaagga agcacaaaaa     3600

ggaaaucaaa ugguccuucc ugaacauuua cucacacucc uauaccaugc uaaacaaugu     3660

uuauuaccaa aucagucuga gucccuaaca uaugucgagc aacaccaacc agaauuucaa     3720

gagauccuag aaagagucgu agauuucgca gaaguacaua cccuagcaaa aucuaaagua     3780

caacagauag uaaagcuauu ugaagcaaac caaaccgcag augucaaaga aauagcagca     3840

aguuuuauac aauuaaugca guucaacgca augggagcac caucaaccuu uaaauucuuc     3900

caaaaagaca uagaacgagc acgauacacg aguauaaaag agauuuucga ugcaacaaua     3960

auauaccaaa guacaacugg cuuauaugaa acuagaagaa aaguaguaga cuga           4014


<210>  5290
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5290
augaagaaag auuacgugau cggacuagau auaggaacga acucagucgg cugggccguc       60

augacugaag auuaccaacu cguuaaaaaa aaaaugccca uauaugguaa caccgaaaaa      120

aaaaaaauaa aaaaaaauuu cuggggagua cgauuauucg aggaaggcca uaccgccgaa      180

gaccgacgac uaaaaagaac cgcucgaaga agaaucucaa gacgacgaaa cagauuaaga      240

uacuuacagg cauucuuuga agaagcuaug acagaccuag acgagaauuu uuuugcuaga      300

uuacaagagu cauuuuuagu accagaagac aagaaauggc accgucaucc aaucuuugcc      360

aaacuagaag augaaguagc auaucaugaa accuauccaa caauauauca ccuacgcaag      420

aaacuugcag acaguuccga acaagcagac cuucgacuaa uauaucuagc auuagcccau      480

auuguuaaau auagaggcca cuucuuaaua gaaggaaaau ugaguacaga gaacacauca      540

guaaaagacc aauuccaaca auuuaugguc aucuacaauc aaacauucgu uaacggggaa      600

agcagacuag uuagcgcacc uuuaccugaa ucuguacuua uagaagaaga auuaacagag      660

aaagcaagua gaacuaaaaa aucagaaaaa guacuacaac aguuccccca ggaaaaagca      720

aacggacuuu uuggacaauu ucugaaauua augguaggaa acaaagcaga uuucaaaaaa      780

guauucggac uagaagaaga agcuaaaaua acauacgcaa gugaaucaua ugaagaagau      840

cuagaaggaa uacuagcaaa aguaggugau gaguacagug auguguuccu agccgcaaag      900

aauguauaug acgcugugga acugagcaca auauuagcag auagcgacaa aaaaucacau      960

gcaaaauuga guucaucaau gaucguuagg uucacagaac accaagaaga ccucaaaaag     1020

uucaaaagau uuauuaggga aaauugucca gaugaauacg auaacuuauu caaaaacgaa     1080

cagaaagacg guuacgcugg auacauugcc caugccggaa aagugucgca guuaaaauuu     1140

uaucaauacg uaaaaaaaau aauacaagau auagccggcg ccgaauacuu ucuagaaaaa     1200

aucgcccaag aaaauuuuuu aagaaaacag cgaacauucg acaauggcgu cauaccacac     1260

cagauacacu uagccgaacu gcaagccauu auacacagac aagccgcuua uuacccauuc     1320

cucaaggaaa accaagaaaa aauagaacaa uuaguaacuu uuagaauccc guauuaugua     1380

ggucccuugu caaaaggaga ugccuccacc uuugcauggu uaaaaagaca aucugaagag     1440

ccgauaagac cauggaauuu acaggaaacc guagaccuag accaaucagc aacagcauuc     1500

auagaacgaa ugacaaauuu cgacaccuac cuucccuccg agaaaguauu accuaaacac     1560

ucuuuauuau acgaaaaauu uauggucuuu aaugaguuaa cgaaaaucuc cuauacagac     1620

gaccgaggaa uaaaagccaa cuucaguggc aaagaaaaag aaaaaauauu cgauuacuua     1680

uucaaaacac gacgcaaagu caaaaaaaaa gacauuauac aauucuaccg aaacgaguac     1740

aauacagaaa uaguaacgcu aucaggacuu gaagaagauc aauucaacgc aucauuuucc     1800

acauaccagg aucuccuaaa augcggucua acaagagccg aacuagauca uccagacaac     1860

gcagaaaaac uugaagacau aauaaaaauc cucacaaucu ucgaagacag acaaaggauc     1920

cggacucagc ucucaacguu caagggucaa uucuccgcgg aggugcucaa aaaauuagaa     1980

cgaaaacauu auaccgguug gggcagauua agcaaaaaac uuaucaacgg caucuacgac     2040

aaagaaagug guaaaaccau cuuagauuac cuuaucaaag augacggggu aucaaaacau     2100

uacaauagaa auuucaugca acuaauaaau gauucgcagu uaucauuuaa aaaugcuauu     2160

caaaaagcac aaagcuguga acacgaagaa acacuaucag aaacaguaaa ugaauuagca     2220

gguucaccug cuauuaaaaa agggauauac caaaguuuaa aaauaguuga cgaauuagug     2280

gcuauuaugg gauacgcccc caaacgcaua guaguugaaa uggcaagaga aaaucaaaca     2340

acaucaacag ggaaacgaag aucgauccaa cgguuaaaaa uaguggaaaa agccauggca     2400

gaaauaggau caaaccuacu aaaggaacag ccaacuacaa augaacaauu aagagacacu     2460

cgauuguucu uguacuauau gcaaaauggc aaagauaugu auacaggcga cgaacuuucg     2520

cuucaccgac uaagucacua ugacauagac cacauuaucc cgcaaaguuu caugaaagac     2580

gauucucuug acaauuuagu acucgucgga ucaacagaaa acagaggcaa aucugacgac     2640

gucccuagca aaaaaguagu aaaggauaug aaagccuacu gggaaaaauu auacgcugcc     2700

ggccucauaa gucaacgaaa guuucaaaga cuaacuaaag gcgaacaagg ugggcuaacu     2760

cuugaagaua aagcccauuu uauacaaaga caauuagucg aaacacgaca gauaacaaaa     2820

aacgucgcag guauccucga ccaaagauac aacgccaaau cuaaagagaa aaagguacag     2880

auuauuaccu uaaaagcauc ucuaacaucc caauucagaa guaucuucgg acuauacaaa     2940

guacgagaag uuaacgacua ccaccaugga caagaugcau accugaacug uguagucgca     3000

acaacacuau uaaaaguaua uccaaauuua gcaccagaau ucguguacgg cgaauacccu     3060

aaauuucaag cuuuuaaaga aaauaaagca acggcaaaaa caauaauuua uacaaaccua     3120

augcguuuuu uuacugagga ugaaccaaga uucacgaaag auucagaaau ucuauggucc     3180

aauucuuauc uaaaaacaau aaaaaaggaa cuaaacuacc aucaaaugaa cauagucaaa     3240

aaaguagaag uccaaaaagg cgguuucagu aaagaaagca uaaagcccaa aggucccagc     3300

aauaaacuca uaccgguuaa gaauggacua gauccacaaa aauacggugg cuucgauucg     3360

ccaguuguug cauauacugu auuauuuaca cacgaaaagg gaaaaaagcc uuugauaaaa     3420

caagaaauuc uaggcaucac cauuauggaa aaaacuagau ucgaacagaa ucccauacua     3480

uuucucgagg aaaaagguuu ucucaggccc agagugcuua ugaaacuacc aaaauauaca     3540

cuuuaugaau uucccgaagg uagaagaaga cuacuagcuu cagcaaaaga agcgcaaaaa     3600

gguaaccaaa uggucuuacc agaacaucua uuaacauugu uguaucaugc uaaacaaugc     3660

cuacucccaa accaaaguga gucucuagca uacguagaac aacaucaacc ugaguuccaa     3720

gaaauacucg aaagagucgu cgauuuugca gagguucaca cacucgcaaa aucaaaagua     3780

caacaaauag uuaaacucuu cgaagccaac cagacagccg acguaaaaga aauugcggcu     3840

ucauuuaucc aauuaaugca auucaaugca augggggcuc cuuccacauu caaauuuuuc     3900

caaaaagaca uugagagagc cagauacaca ucaauaaaag aaauuuucga cgcuacaauc     3960

aucuaucaau cuacaacggg ccuauacgaa acgagacgua aaguuguaga cuga           4014


<210>  5291
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5291
augaagaaag acuauguaau aggauuagac auaggaacaa acucaguagg augggcgguu       60

augacagaag auuaccagcu agucaaaaag aaaaugccaa uauacgguaa uacggagaaa      120

aagaaaauca agaaaaacuu uuggggugua agacuauucg aagaagguca uacagccgaa      180

gaccgcagau uaaaaagaac agcacgacgg cgcauaagcc gacgaagaaa ucgccuaagg      240

uacuuacaag cguucuuuga ggaagcgaug acagcauuag augagaauuu cuuugcaaga      300

cuacaagaaa guuuucuugu cccagaagac aagaaauggc auagacaccc gauauucgcu      360

aaacuagaag acgaaguagc auaccacgaa acauacccaa ccauauacca ucuccgaaag      420

aaacuggcag auuccagcga acaagcagac cucagacuaa uauaccuugc auuagcacau      480

auuguaaaau accgcggcca uuuccuuauu gaaggcaaau ugagcacaga aaacaucucu      540

gugaaagagc aauuccaaca auuuaugaua auauacaacc aaacauucgu uaacggcgaa      600

aguagacugg uauccgcacc acucccggaa agcguuuuaa ucgaagaaga acuuacagaa      660

aaagccucaa gaacaaagaa auccgaaaaa guacugcagc aauuucccca agaaaaagca      720

aauggacuau ucggacaauu cuuaaaacuu augguaggua auaaagcuga cuucaagaaa      780

guauuuggac ucgaagaaga agcaaaaaua acauacgcau cagaaucgua ugaagaggac      840

uuagaaggca uucuagccaa aguaggagac gaauacucag acguauuucu agcggccaaa      900

aaugucuaug augcaguaga acuauccacu auacuagcag auagcgauaa gaaaagucac      960

gcuaaauuau ccagcuccau gauuguacgc uucacagaac aucaagaaga ccuuaaaaac     1020

uucaaaagau ucauacgaga aaauugcccg gacgaauacg acaacuuauu caaaaaugag     1080

caaaaagaug gauaugccgg auauauaacc caugcaggaa aagucagcca acucaaauuc     1140

uaucaauacg ugaagaaaau cauacaagau auagcaggag cagaauacuu uuuggagaaa     1200

aucgcacaag aaaauuuccu ucgcaaacaa agaacauucg acaauggcgu cauaccucac     1260

caaauacacu uggccgaacu gcaagcaauc auacaucgac aagccgccua uuauccguuu     1320

uuaaaagaaa accaagagaa aauagaacaa uuaguaacau uccgcauccc guauuacguc     1380

ggaccccuau ccaaaggaga ugccaacaca uuugcguggc uuaaacgaca aagugaagaa     1440

cccauaagac cguggaaucu ccaagaaaca guagaccuag accagucagc cacugcguuu     1500

auugaaagaa ugacaaacuu ugacaccuac cucccaucag aaaaagucuu accgaaacac     1560

ucccuauuau acgaaaaauu uauggucuuc aacgaacuua caaaaauaag cuacaccgau     1620

gacaggggaa uaaaagcaaa cuucucagga aaagaaaaag agaaaauauu ugacuaccua     1680

uucaaaacca gaagaaaagu uaagaagaaa gacaucauuc aauucuacag aaacgaauau     1740

aauacagaaa ucguaacccu gucaggucuu gaagaagacc aauuuaacgc gucauuuucu     1800

acauaccagg auuuauugaa augcggacuu acuagagcgg aacuagacca cccagacaau     1860

gcggagaaac ucgaagacau aaucaaaaua cugacaaucu uugaggauag gcaaagaaua     1920

cggacucaac uuagcacauu caaaggccaa uuuucggcug aaguauugaa gaaacuugaa     1980

agaaaacauu acacaggaug gggaagauua agcaagaaac uaauuaaugg aaucuacgau     2040

aaagaaucag gaaaaacaau acuagauuau cuaauuaaag augacggggu aucuaaacau     2100

uacaacagga auuucaugca acuaauaaac gacagucagc uuucauuuaa aaaugcaaua     2160

caaaaagcuc aaucgucaga acaugaagaa accuuaagug aaacaguaaa cgaauuagca     2220

ggaagucccg caauuaagaa agguauauau cagagucuaa aaauagucga ugagcuagua     2280

gcgauuaugg gauacgcacc aaaacgaaua guuguagaaa uggcccgaga aaaccagaca     2340

acauccacag gaaaaaggag aagcauucaa cgucuaaaaa uaguugaaaa ggcaauggcu     2400

gaaauaggcu caaaccuauu aaaagaacaa ccuacuacaa augaacaacu acgugacaca     2460

cgacuauucc uauacuauau gcaaaaugga aaagacaugu auacagguga ugaacuauca     2520

cuccaccguu ugagccacua cgauauagac cauaucaucc cucagucauu uaugaaagac     2580

gacucacuug auaaucuagu acucgucggc ucaacagaaa accgcgguaa aagugaugac     2640

guaccaagca aagaaguagu caaagacaug aaggcuuacu gggaaaaacu uuacgccgcc     2700

ggacucauua gccaaaggaa auuccaacgc cuaacaaaag gcgaacaagg cggucuaaca     2760

uuagaagaca aagcacauuu uauacaaaga caauuaguag aaacacggca aauaaccaaa     2820

aauguugccg gaauauuaga ccaaagauau aacgcaaaau ccaaagaaaa gaaaguacag     2880

aucaucacac uaaaggcuuc acuuacauca caauuccgaa gcauauuugg auuauacaaa     2940

guaagggaag uaaacgauua ccaccacgga caagacgcgu accuaaauug cguaguugcu     3000

acaacguuac ucaaagugua uccaaacuua gcaccugagu uuguauacgg agaauaccca     3060

aaauuccaaa cauuuaaaga aaauaaagca acugcaaaag cuauaauaua cacaaaccua     3120

uuaagauucu uuacagaaga ugaaccacga uuuacaaaag acggugagau ucucuggucu     3180

aacuccuauc ucaaaacaau uaagaaagaa uuaaauuauc aucaaaugaa uauagugaag     3240

aaaguagaag uacaaaaagg agguuuuagc aaagaaagua uaaaaccaaa aggaccaagc     3300

aacaaacuca uacccgucaa aaauggacua gacccacaga aauauggugg auucgacuca     3360

ccaauaguag cauauacagu cuuauucaca caugaaaaag gaaagaaacc cuuaaucaaa     3420

caggaaauau uaggaauuac aaucauggag aaaacgcgau ucgaacagaa uccaauuuua     3480

uucuuagaag aaaaaggauu cuugagacca cgcguccuua ugaaauuacc aaaauacaca     3540

cuauaccaau uuccugaagg ccguagaaga uuauuagccu cagcaaaaga agcacaaaaa     3600

gguaaccaaa ugguccuacc ugaacaucua cuuacucucc ucuaucaugc aaaacaaugc     3660

uuacuaccua accaaucaga aucacuagca uacgucgagc aacaucaacc agaauuucaa     3720

gaaauacuag aacgaguggu cgauuucgca gaagugcaca cuuuagcuaa aucaaaagua     3780

caacaaauag uaaaauuguu ugaggcaaau caaacagccg auguaaaaga aauagcagcc     3840

agcuucauac aauuaaugca guucaaugcu augggugcac caagcacuuu caaauuuuuc     3900

caaaaagaca uagagagagc aagauacaca uccauaaaag aaauauuuga cgcuacaaua     3960

aucuaccaaa gcaccacugg auuauaugaa acacgacgaa aagucguaga uuga           4014


<210>  5292
<211>  4014
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5292
augaaaaaag acuauguaau aggacuagac auuggcacaa acaguguugg cugggcugua       60

augacagaag auuaucaguu aguaaaaaaa aaaaugccca ucuauggaaa caccgaaaaa      120

aaaaaaauua aaaaaaauuu cuggggaguu agauuguuug aggaagggca cacagcugaa      180

gauagaagac uuaaaagaac cgcuagaaga cgaauuucua gacggagaaa cagacuuaga      240

uacuuacaag cauucuuuga agaagcaaug acagacuuag acgaaaauuu uuucgcgcgu      300

cuacaagaau cauuucuugu uccagaagac aagaaauggc acagacaccc aauauucgcc      360

aaacuagagg acgaagucgc uuaccacgaa acuuacccaa caaucuauca cuuaaggaaa      420

aaacuagccg acucuucaga gcaagcagac cuaagacuca uuuaccuugc ccuagcccac      480

auuguuaagu aucgaggcca cuuccuaauu gaggggaaau uauccacgga aaacaucuca      540

guaaaagagc aauuccagca auucaugauc aucuauaacc agacauuugu aaauggagag      600

agcagacuag uaagugcucc auugccagaa ucaguuuuaa uugaagaaga acuaaccgaa      660

aaagcaucaa gaacgaaaaa gagugaaaaa guacuacaac aauuucccca ggaaaaagcu      720

aauggauuau ucgggcaauu ucuaaaacuu augguaggaa acaaagccga uuuuaaaaaa      780

guauuuggac uagaagaaga agccaaaauc acauacgcuu cugaaagcua ugaggaggau      840

uuagaaggaa uacuagcaaa aguaggcgau gaauauagcg auguauuccu ugcagcaaaa      900

aauguauaug augcaguaga auuaagcacu auacuggccg auagugacaa aaaaucccac      960

gcaaaacugu cgaguaguau gauaguuaga uucacugaac aucaagagga ccugaaaaaa     1020

uuuaaacgcu ucauacgcga aaacugcccc gaugaauaug acaaucuauu uaaaaaugaa     1080

cagaaagaug guuacgccgg auacauagca caugcaggua aaguaucgca auuaaaauuc     1140

uaccaauacg ucaaaaaaau uauucaagac auagcgggag cagaauacuu ccuagaaaag     1200

auugcucaag agaauuuccu gaggaaacaa cgaacauucg acaacggagu aauaccacac     1260

caaauacauc uagcagagcu acaagcaauu auccacaggc aagcagccua uuaccccuuc     1320

uuaaaagaaa accaagaaaa aauagagcaa cucguuacau uuagaauacc auauuaugua     1380

ggaccacuua gcaaaggaga ugccagcaca uucgcauggc ucaaacgaca aucagaagaa     1440

ccuauaagac cauggaaccu acaagaaaca guagacuuag aucaaucggc cacagccuuu     1500

auugaacgaa ugacuaauuu cgacacauac uugccaucug aaaaaguacu accaaaacau     1560

ucauuacucu acgaaaaauu caugguauuc aacgaacuga cgaaaauauc uuacacagac     1620

gaucggggaa ucaaagcuaa uuucucuggu aaagagaaag aaaaaauauu cgacuacuua     1680

uucaaaacac gcagaaaagu gaagaagaaa gacauuauac aguucuaucg aaaugaauac     1740

aauacugaga uuguaacucu aucaggauua gaagaagauc aauuuaaugc aagcuuuuca     1800

acauaccaag accucuuaaa augcggacua acacgagccg aauuagacca cccggacaac     1860

gcugagaaau uagaagacau caucaagaua cucacaauau ucgaggaccg acaaagaauu     1920

agaacacaau ugagcacuuu caaaggacaa uucucagccg aaguucucaa gaaacucgaa     1980

agaaaacacu acacaggaug gggaaggcug ucaaaaaaac ucauaaacgg cauuuacgac     2040

aaagaguccg gaaagaccau acucggauau cuaauaaaag augauggagu gaguaagcac     2100

uauaauagga auuuuaugca acucaucaac gauucacaac uuagcuucaa aaacgcaaua     2160

caaaaagcuc aaucaaguga acaugaagaa accuugucag aaacuguaaa ugaacucgcg     2220

ggaagcccag cgauaaaaaa agguauauau caaagcuuaa aaauaguuga ugaguuaguc     2280

gcaauaaugg gauacgcgcc caaacgaauc guuguagaaa uggcaaggga aaaucaaacu     2340

accagcacag gaaaaagacg aucaauacaa agacuaaaaa uuguagaaaa ggccauggcc     2400

gaaaucggua gcaaucuccu uaaagaacaa ccaacaacaa acgaacaacu uagagacacu     2460

agauuauucc uuuauuauau gcaaaacgga aaagacaugu auacaggaga ugaacucuca     2520

cuacaucgau uaagccauua cgacauagau cacauaauac cccagaguuu uaugaaagac     2580

gauaguuuag auaaccuugu acuaguaggc ucaacugaaa auagagguaa aucagaugau     2640

guuccaucua aagaaguagu aaaggacaug aaagcauauu gggaaaaacu auaugcugcg     2700

gggcuaauaa gccaaagaaa auuccaacga cuaacaaaag gcgaacaagg cgggcucacg     2760

cuugaagaua aggcacacuu cauacaacgu caacuagucg aaacaagaca aauaacaaaa     2820

aacguagcag gaauccuaga ccagcgguac aaugcaaacu caaaagaaaa aaaaguacaa     2880

auuauaacac uaaaagcauc acuuacauca caauuuagau caauauucgg ucucuacaaa     2940

gugcgugaag uaaacgauua ucaccacggc caagaugcuu acuuaaauug uguuguugcu     3000

acuacacuac ucaaagugua ucccaaccua gcaccagaau ucguuuaugg ugaauaccca     3060

aaauuccaaa cguuuaaaga gaauaaagca acggcaaaag cuauuauaua cacaaaucuc     3120

cuucgcuuuu uuacggaaga cgaaccaaga uuuacuaagg auggugaaau acuauggagu     3180

aauagcuacu uaaaaacaau aaaaaaagaa cuaaauuacc aucagaugaa cauagucaaa     3240

aaaguugagg ugcaaaaggg cggauucuca aaggagucua uuaaaccaaa ggggccuucu     3300

aacaaauuaa ucccagugaa aaauggcuug gacccucaaa aauacggagg uuuugacuca     3360

ccaauagucg cuuauacagu ucuuuucacc caugaaaaag guaaaaaacc auuaauaaaa     3420

caagagauac uaggcauaac cauuauggaa aaaacgaggu ucgaacaaaa uccaauacua     3480

uuccuagaag aaaaaggcuu ccuaagacca cgaguacuua ugaaacuacc aaaauacaca     3540

uuauacgaau ucccagaagg ucggagaagg cuacuagcaa gcgcaaaaga ggcucagaaa     3600

gguaaccaaa ugguuuugcc agagcaccua cuaacacuau uauaccacgc gaaacaaugu     3660

uuauuaccua accaaucaga aagucuugca uacguugaac aacaccaacc ugaauuccaa     3720

gaaauacuag aaagagucgu agacuucgca gaaguccaua cauuagcaaa aaguaaaguc     3780

caacaaauag uuaaacuauu cgaagccaau caaaccgccg auguuaaaga aaucgcugcc     3840

aguuucauac aauuaaugca guuuaacgcu augggagcuc cuaguacauu caaauuuuuu     3900

cagaaagaca uagaaagagc cagauauaca ucaaucaaag aaauauucga cgcaacuauu     3960

auauaucaau cuacaaccgg auuguacgag acaagacgaa aaguaguaga cuga           4014


<210>  5293
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5293
augcaagacu acuaucucgg acuagacaua ggcacaaaua gcguagggug ggcaguaacg       60

gaugaaaacu acaaucuacu cagaaaaaag gguaaagacc uauggggcgu uaggcuauuc      120

aaagaagcgc aaccagcuac ugaacgcaga ggacauagaa guagcagaag acgcaccauu      180

agaaaaucac uacguaaaca auuacugaga gagcucuuug aagaagagau caacgcaauc      240

gauaaagacu ucuuccuacg acuggacgaa ucaaaauacu gguuagaaga caaaaagaua      300

gaaggaaaau acagcuuguu uaacgaugag aauuucacag acaaagauua cuaugaaaag      360

uuuccgacaa uuuuccaccu acgaaaggca cuaauagaau cagacgacaa agaagacauu      420

agacucuacu uccuugcaau aaaucaaaug augaaaagac guggccauuu ccugauugac      480

ggaaaacucu cuaacguaga agauauaaca ccauucaaag aauguaucca aaacuuaaua      540

aacgaagaau uagauaccga aauagagacc gaauucuacg acaaagcaau caaaauagug      600

ucagacuaca gcuuaaaaaa aacugaaaaa aaaaacuuaa uaaaagacuu agucaaagaa      660

acagauucca uagaagacuc aaaaaaaauu aucaauauau ucaaucuauu aaucaguggu      720

aaauccaaag uaaacggaau cuucgaugac gaagaaauac uugaaaagau caaagaagau      780

aauaaagaag acuucuauuu aacgagugag aaauaugaag aaaaucuaga auacuucagc      840

caauuacucg auuaccgauu cgaacuaaua ucaaguuuaa agucaauaua cgacuacauu      900

auccuacaag aaguacucaa aggaaaaaaa uacuugucau acgcgcaagu agagcguuac      960

aaaaaccaua aagaagaucu aaggaaacuc aagcaacuca uaaagaaaua cgauaaaaac     1020

ggcaaaaucu acuaccaaau cuucagggac gaaaacuuca aaaagggcua uguagcuuac     1080

cuagguuauu acaaaaagaa uaaaaaaaau guaccuguaa aaaaauguga cuaugacacg     1140

uuuaguaaaa acauaaaauc aacacuggac aaaauaugug acaaggaaga cccacucuau     1200

auagaacuaa uagacaaaau agagaaagga ggauuccuuc uaaaacaaau aguaucaacu     1260

aacucaguaa uaccucauca agugcaugag cuagaauuag acaaaauacu agaaaaccuu     1320

gaaaaaaauu auccaaguuu uaaggaaauc ucagauggcg uaaguaaaau ugauaagaua     1380

aaaaugauuu uuaacuuuag aauaccauac uauguuggcc cccuuaacga cuaucacaaa     1440

gacaaaguaa acagcaacac guggaucguu aaaaacgaag gauuugagaa ugaaaacaua     1500

cgaccuugga acuuuucaaa aaucguagac gaacaugcau gcgaagaaga auucaucaaa     1560

cgaaugauua augaauguuc auacauacca ggcgaaaaag uccuaccaaa aagcucauua     1620

uuauauucag aauacauggu auuaaacgaa cucaauaacc uacgcguuaa uggagacaaa     1680

cuaaacccca agaucaaaaa ggaaauaguu gaaaacauau acaaaaaaga acgaaaagua     1740

acgcucaaaa aaauaagaaa uuuuuuaaua auaaacaauu acgucgacuc caagguagaa     1800

uucucuggau uaagcgacgg aaucaaagca aauaugucaa gcuacaucga uuuugcggaa     1860

auccuugacg auaacuuuga caacaauaug guagaagauu uaauagaaca aauaacaauu     1920

cauguaggca acguaaaacu acuaaccgaa aaaauaucaa auaaauaccc aaaucuugaa     1980

aaaaaacaaa ucaauaaaau uguaaacuua aaguuuaaag auugggcaag guucucaaga     2040

aaguuauuag uaggaauaga aggaacagaa aaagaaaccg gagagaucaa uacaauaauu     2100

ggauuucuac gugaaacuaa cgaaaacuuc auggaauuaa ugagcaaucg auacacuuuc     2160

agcgaagaaa ucagugaaau caaaaaaaaa uacauaccua auaaauuauc uaagcaaauc     2220

guggacgauc ucuacguauc acccgcagua aaaaaaauga uauggcaagu acuaaacauu     2280

aaugacgaac uaauaaaagu acuaggaaaa cagccaaaga aaaucuucau cgaaauggca     2340

cgcgggaaag aagagaaacc agaaguaaaa gacucaagga aaaauaaacu cauagaauua     2400

uacaaaaacc uaggaaaaga agguaaagaa uggcuaaaua gaauugaaga uaaaaccgaa     2460

gccgacuuca gaaaucgugc cuuauaccua uacuacacuc aaaugggaag auguauguac     2520

acaggagaaa uaauagacau ugaacguuua uacgacaaag aauuauacga ccucgaccac     2580

aucauaccuc gcaaaaugaa aaaggacgau uccauacaca aaaauuuagu acuaguaaca     2640

agaaacagua accaauacga aaaaaaagac aauuacccag uaccggaagu aauaaaaucu     2700

agagaaaaug uacgucaccu cugguaugca cuaaaaaaaa agggccuaau cacagaggag     2760

aaauacaauc gacuuacucg uaucaaccca cucucagaua aagaacuauc agaguucaua     2820

gaaagacaac uaguggaaac aaggcaaaca acaaaagucg uaaaagaccu cuuugaaaac     2880

uacuacacag acucgaaaau agucacagug aaagcagggc uagucucuga acugcgacga     2940

gauuuugaag uucuaaaaug cagagaaaua aaugaccuca accacgccca ugacgcauuc     3000

cuuaauauca uaguagguga cguauggagc aaaaaauuua ccuccaaccc auauaauuuu     3060

gucaagaaaa accgagagaa auacacauua aacaacaucu ucacuaaaaa ucaaaaagau     3120

cuacgaggga acgaaauuug gaacugcgca gaagguaaaa aaagaauaau uaaaacauua     3180

aacaaaccau cuguccuauu cagcguagaa aacuucgaac aaaccgguca acuauuugac     3240

caaaauauca uaggcaaaca agacuauaaa gaaaaaacaa gcuauguucc aauaaaagga     3300

gacgaaagau uacaagacau aucgaaauau ggaggauacc gaggaauauc aggcgcguac     3360

uuuuucaucg uagaacauac cgaaaaaaau aaaagaguca aaacaaucga aagccuacca     3420

auauacauga aaaguuaugu agaagagaau ggcuugcuag acuucuguac aaaugaaaug     3480

aaauuaguag acccuaaaau aauagugaaa aaacuaaacu acaaaucuga aauaauaguu     3540

gacggauuca aauaucucau aucaggaaag acagaaaacc gauuaagcuu agaaacaaau     3600

gaauaucucu auuggaauca uacccaaaca acagacauaa aaaagauccu aaauauaaau     3660

gaaaaaauag agcuugaaaa auacaacgga gauauagaga gugaacgaga aaacauaaua     3720

aaauacauac aaauaauauu agagaaauuu aaacuuaagc cauucauaaa gcgacgaaac     3780

cuuccaaaca uagagaucaa uuucgcagau uacgaaaccg aaaaauuacu acaauuacuc     3840

gucaaccuca ucccacucac aacuaaauac gucaguccag uagaucucac aguaaucagc     3900

ggauccaaau augccggcaa aguccuaauc ccgaaaaaga uaaguaaucu caacgaaguc     3960

auauucauau cuaaaagcau aacaggaauc uaugaaucac gccguagaau uauauga        4017


<210>  5294
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5294
augaaacaag aauauuucuu aggacuugau augggcacag gaagucuagg augggccgua       60

acagacagca cguaucaagu caugagaaaa cauggaaaag cacuaugggg aacgagacua      120

uuugaaucag caucaacagc cgaagaacgc agaauguuua gaacagcaag acgaagacug      180

gacaggcgaa auuggcgaau acaaguauua caagagauau uuucagaaga aauaucaaaa      240

guagauccgg gauucuuuuu aagaaugaaa gaaagcaaau acuacccuga ggacaaaaga      300

gacgcagaag gaaauugucc agaauuaccc uacgcgcuau uuguagacga caauuacacc      360

gauaagaauu aucacaaaga cuaucccacg auauaucacc ucagaaaaau gcuaauggag      420

acaacugaaa uuccagauau uagauuagua uacuuaguac uccaucacau gaugaaacau      480

cgaggacacu uucuucucag cggagauaua ucacaaauua aagaguucaa auccacuuuu      540

gaacaguuaa uacagaacau acaagaugag gaacuugaau ggcacauaag ucuagaugau      600

gcugcuauac aguuuguaga gcacguacua aaggacagaa acuuaacaag aucaacaaag      660

aaaucaagac uaauaaaaca acucaaugcc aaaucagcau gugaaaaagc aauacuaaac      720

cuacuaucag gcgguacagu caaauugagc gauauauuua acaacaaaga acuggacgaa      780

agugagagac caaaaguauc auucgcagac ucuggauaug acgacuauau uggaauagua      840

gaagcugaac uugcagaaca guacuacauc auagccagug cgaaagcagu uuaugacugg      900

ucaguccucg ucgaaauacu gggaaacagc guaucaauua gcgaagcaaa aauaaaagua      960

uaucagaaac accaggcaga cuuaaaaacu uugaaaaaga uagucagaca auauaugacc     1020

aaagaagauu acaaaagagu auuuguagau acagaagaaa aauuaaacaa uuauucagca     1080

uacaucggua ugacuaagaa aaauggaaag aaaguagacc uuaaaucaaa acaaugcacc     1140

caggcagauu uuuaugauuu ccuaaagaaa aacguaauaa aagucaucga ccauaaagaa     1200

aucacacaag aaaucgaaag ugaaauugaa aaagaaaacu uccuaccaaa acaaguuaca     1260

aaagacaaug guguaauccc auaccaggua cacgauuaug aauuaaagaa aauauuagac     1320

aauuuaggga caaggaugcc uuucaucaaa gaaaaugcag agaaaauaca acaacuauuu     1380

gaauuucgga uuccuuauua cguagguccu cuaaacagag uugacgacgg uaaagaugga     1440

aaauucaccu ggaguguaag aaaaucugau gcacguauau aucccuggaa uuucacagaa     1500

guaauugaug uagaagcauc cgcagaaaaa uuuauacgaa gaaugacaaa caaaugcaca     1560

uauuuaguag gcgaagacgu gcuaccgaaa gacucauuag uguauuccaa auuuauggug     1620

uuaaaugaau uaaauaauuu gcgguuaaac ggggagaaaa uaagugucga acuaaaacaa     1680

agaaucuaug aagaacuauu uugcaaauau agaaagguaa cacgaaagaa auuagaacgc     1740

uaucuaguaa uagaaggaau agcaaagaaa ggagucgaaa uuacaggaau agacggcgac     1800

uucaaagccu cgcucacugc auaucacgau uucaaagaac gucugacgga cguacaacua     1860

ucucaaaggg caaaagaagc uauaguauua aacgucguac ucuucggcga cgauaagaaa     1920

uuacuaaaac agcgacuauc aaaaauguac ccaaaccuaa caacaggaca acuaaaaggg     1980

auuuguucac uuaguuacca aggcuggggc agacuuagca aaacguuccu cgaagaaaua     2040

accguacccg cgccgggaac uggugaaguu uggaacauaa ugacugccuu auggcaaaca     2100

aacgacaacu uaaugcaauu auuaucaaga aacuacgguu ucacaaacga aguagaagag     2160

uucaauacac ugaagaaaga aacagacuua aguuacaaaa cuguagauga auuauacguu     2220

uccccugcug uaaaaagaca aaucuggcaa acccuaaaag uuguaaaaga aauucaaaaa     2280

gucaugggua acgcaccaaa aagaguauuc guggaaaugg cccgcgaaaa acaagaagga     2340

aaaaggagug acucaagaaa gaaacaauua guagaacuuu acagagccug uaaaaaugaa     2400

gaaagagacu ggauaacuga guuaaacgca cagucagacc aacaacugag aucggauaaa     2460

uuauucuuau auuacauaca aaaaggacga uguauguacu caggggaaac gauacaauua     2520

gacgaauuau gggacaauac aaaauaugac aucgaccaua uauacccgca aucaaaaacu     2580

auggaugacu cgcugaauaa uagaguacuu guaaagaaaa auuauaacgc aauaaaaucu     2640

gacacuuacc cccuuucacu cgacauccaa aagaaaauga ugucauucug gaaaaugcuu     2700

caacaacaag gguuuauaac aaaagaaaaa uaugucaggc uaguccgauc agaugaacug     2760

agcgcagacg aauuagccgg cuuuauagaa agacaaauag uagaaacacg ucaaucuaca     2820

aaagccgucg caacuauccu uaaagaagca cugccagaca cagaaauagu auaugucaaa     2880

gcugguaaug uaucaaacuu ucgacaaacg uaugaacuuc uaaaaguuag agaaaugaac     2940

gaccuucauc acgcaaaaga cgcuuaucua aacauaguug ucggaaaugc cuauuucguu     3000

aaauuuacca aaaaugccgc cugguucaua agaaauaacc caggcagaag uuacaacuua     3060

aaacgaaugu ucgaguucga uaucgaaaga ucuggggaaa uagccuggaa agccggaaau     3120

aaaggcagua uaguaacagu aaagaaaguu augcagaaaa auaauauacu uguuaccagg     3180

aaagcauaug aaguaaaagg aggacuuuuc gaucaacaaa uaaugaagaa aggaaaaggu     3240

caaguuccaa uaaaaggaaa cgaugaacga cuagcagaua ucgaaaaaua uggagguuau     3300

aacaaagcag cagguacaua uuucauguua guuaaaucau uagacaagaa aggcaaagaa     3360

auaagaacua uagaguucgu cccucuuuac cuaaagaacc aaauugaaau aaaucaugaa     3420

ucagcgauac aauaucuagc acaggaacga ggccuaaaca gcccagaaau acuauuaagu     3480

aaaauaaaaa uugauacacu cuuuaaagua gacggguuua aaaugugguu aagcggcaga     3540

acagguaacc aauuaauuuu caaaggagca aaucaacuca uacuaucaca ucaagaggca     3600

gcaauauuaa aaggaguggu gaaauaugua aaccgaaaga acgaaaauaa agaugcaaaa     3660

uuaagcgaaa gagacggaau gaccgaagaa aaauuacuac aauuauacga uaccuuucuu     3720

gacaaacuaa guaacacagu auacagcaua aggcucucag cacaaauaaa aacacuaaca     3780

gagaaacgag cuaaauucau aggacuuagu aaugaagauc aauguauagu acucaaugaa     3840

auucuacaua uguuccaaug ucaaucagga ucugcaaacu uaaaacucau uggcggcccu     3900

ggauccgcag gaauauuagu aaugaacaau aauauuacag cuuguaaaca aauaucugug     3960

aucaaccaau caccaacagg caucuacgaa aaagaaauag accuaauaaa acuauga        4017


<210>  5295
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5295
augacuaaac cauacaguau cggauuggac auuggaacca acaguguagg augggcuguu       60

guuacagaug guuacaaagu ccccgcuaaa aaaaugaaag uauuaggaaa cacugauaaa      120

agccacauca aaaaaaaccu gcuuggagcc cuauuauucg acagcggaaa caccgcaaaa      180

gaccgcagac ucaaacgcac agcuagaaga agauauacaa gacgccgaaa caggauccuu      240

uauuuacaag aaaucuuugc ugaagagaug gcaaaagcag acgaaucguu uuuucaaaga      300

uuaaaugaau cuuuucuaac caaugacgac aaagaauucg acucacaccc uauuuucggu      360

aacaaagcug aagaagaagc gcaccaccac aaguuuccca caauauuuca ccuaagaaaa      420

caccuagcag acucaacaga aaaaagugac uuaagauuaa uauaccuagc auuggcacau      480

augauaaagu uuagagggca cuuuuuaaua gaaggccaac uaaaagcuga aaauacaaau      540

gugcaaaccc uauuugacga uuucguagaa gucuacgaca aaacaguaga agaaucacac      600

cuuucagaga uuucgguauc cucaauacuu acagaaaaaa uuaguaaauc aagaagacua      660

gaaaaucuaa uaaaauacua uccaacagaa aaaaaaaaua cacuauuugg uaaccuaaua      720

gcguuaaguc uagguuuaca accgaacuuc aaaacuaacu uuaaacucuc agaagaugca      780

aaauuacaau ucucaaaaga cacauacgaa gaagacuuag gagaacucuu ggguaaaaua      840

ggagauaauu acgcagaucu auucauaucu gcaaaaaacu uauaugacgc uauauuacua      900

uccggaaucc uuaccauaga cgauaauaca acaaaagcac cauuaagugc gaguaugaua      960

aagagguacg aagagcacca agaagauuua gcucaauuga aaaaauucau aagacaaaac     1020

cuaccagauc aauauuccga gguauuuuca gacaaaacca aagaugguua ugcuggauau     1080

auagacggga aaaccaauca agaagcauuu uauaaauaua uuaagaacau gcuaucuaaa     1140

accgaaggcg cggacuacuu ccuugauaaa aucgacagag aagacuuucu aagaaaacaa     1200

cgaacauucg acaauggauc uguuccgcac caaauccauc uacaggaaau gcaugccauc     1260

cucagacguc aaggagaaua cuacccauuc cugaaagaaa accaagauaa aauagaaaaa     1320

auacuuacgu ucagaauccc cuacuaugua ggcccuuuag cccgcaaggg cucgcgcuuu     1380

gcuugggcag aguauaaagc agacaaaaag guuacaccau ggaacuuuga cgacauauua     1440

gauaaagaaa aaagcgcaga agaauucauu acuagaauga cguuaaauga uuuauaucuc     1500

ccagaagaga aaguauuacc aaagcauucg uuaguauacg agaccuucaa cguauacaau     1560

gaacucacua aaguuaaaua ugucaacgaa caagguaaag caaucuucuu ugacgcuaac     1620

augaaacaag aaauauucga ucauguuuuu aaagaaaacc gaaaaguaac aaaggacaaa     1680

cuacuaaauu auuuaaacaa agaauuugaa gaauucagaa uaguaaauuu aacaggauua     1740

gauaaagaaa acaaagcauu caauucaucc cuagguacau accacgaccu aagaaaaauc     1800

uuagauaaau cauuccuaga cgauaaggua aaugagaaaa uaauugaaga uauaauacaa     1860

acucuuaccu uauuugaaga uagagaaaug auuagacaaa gauugcaaaa auacucagau     1920

auuuuuacua cccaacaacu caaaaaacua gagcgaagac acuacacagg guggggccgc     1980

uuaagugcua aacucauaaa cggcauaaga gacaaacaau caaauaagac aauacuagga     2040

uauuuaaucg acgacgguua uucaaaucga aacuuuaugc aacucaucaa ugaugauuca     2100

uuaccauuua aagaagaaau agcucgagca caagucaucg gagaaacuga ugauuuaaac     2160

caauuaguga gcgauauagc cggcucucca gccauaaaaa aaggcauacu acaauccuua     2220

aaaauaguag augaguuagu aaaagugaug ggccauaauc ccgcuaacau uguaaucgaa     2280

auggcacgcg aaaaccaaac gacagcuaaa gguagacguu cgucacaaca acgauauaaa     2340

cgauuagagg aagcaauaaa aaaccuggac caugaucuaa aucauaaaau acuaaaagaa     2400

cacccgacgg auaaccaggc ucuacaaaac gaccgccuau ucuuauacua ccuacaaaac     2460

ggaagggaca uguauacuga agauccgcua gacaucaauc gcuuaagcga cuaugacaua     2520

gaucauauaa ucccccaauc cuuuaucaaa gacgacagca uugacaauaa agucuuaguu     2580

ucuucagcaa aaaacagagg uaaauccgac aauguaccau cagaagaugu cguaaaucgc     2640

augagacccu ucuggaacaa acuauuaucu ugcggacuca ucucacaaag aaaauauuca     2700

aacuuaacaa aaaaagaacu uaaaccagac gauaaagcag gauuuauaaa aagacagcua     2760

guagagacac gacaaaucac aaaacacgua gcgcaaauac uggacgcaag auucaacaca     2820

aaaagagaug aaaacaaaaa gguuauaaga gaugucaaaa uaauuacucu uaagucaaac     2880

uuaguaagcc aauuccgaaa agacuuuaaa uuuuacaaag uaagagagau aaacgauuau     2940

caucaugcac augaugcaua ucuaaaugcc guaauaggaa aggcacucuu agacguauau     3000

ccgcaacuag aaccugaguu ugucuacggu gaauauccuc acuuccaugg auacaaagag     3060

aauaaagcaa cugcgaaaaa auucuuuuau ucuaacauca ugaacuucuu caaaaaagac     3120

gacauaagaa cagaugaaaa uggugaaaua guuuggaaaa aagacgaaca cauaucaaau     3180

auaaagagag uacuaaguua cccucaggua aacauaguca aaaaaguaga aauacagacc     3240

guaggacaaa auggcgggcu auucgacgac aacccuaaaa gcccacuaga gguuacuccu     3300

aguaaacucg uuccccuaaa aaaagaauua aauccaaaaa aauacggggg cuaucaaaag     3360

ccuaccaccg cauacccagu auuauuaauc acagauacaa agcaauuaau accaauauca     3420

gugaugaaua aaaaacaauu ugaacaaaac cccguaaaau uccuuagaga ucgaggguac     3480

caacaaguag gaaaaaacga cuucauaaaa uuaccaaaau acacacuagu ugauauagga     3540

gacggcauaa agagacuaug ggccagcucg aaagaaauac acaaaggaaa ccaauuaguc     3600

guaaguaaaa aaagccaaau ccuacuauac cacgcacacc accuugacuc agaccucuca     3660

aaugacuacc ugcaaaacca uaaucaacag uucgauguau uauucaauga aaucauaucc     3720

uuuucaaaaa aaugcaaauu aggaaaagag cacauacaaa aaaucgaaaa cguguauagc     3780

aauaaaaaaa acucugcauc cauagaggaa uuagcagagu cauucauuaa acuacucggc     3840

uucacucaac uaggcgcaac cucaccguuu aacuuuuuag guguaaaacu aaaccaaaag     3900

caauauaaag gcaaaaagga uuauauacuc ccaugcacag aaggaaccuu aauuagacaa     3960

uccauuacag gauuauacga aacaagagug gaucuaagca aaauaggaga agauuga        4017


<210>  5296
<211>  4017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5296
augcgcgacu auuauauagg acuagaccua ggaaccggau cacuaggcug ggccguaaca       60

gaucgagaau augaaaucau gagagcacac ggaaaagcau uauggggagu uagacucuuc      120

gauuccgcca auacggcuga agagcgcaga ggcuucagga cagcacgacg aagauuagac      180

aggagaaauu ggaggauaga acuccuacaa gaguuauuug gugaggauau aggcaaagua      240

gauagugggu uuuuccugcg caugaaagaa ucaaaauaca ugccagaaga caaaagagau      300

guuaacggua acugcccaaa auuaccuuac gcacuuuuug uagaagaugg uuauacagac      360

aaagauuauc accgucaauu uccgacaauu uaccaucuca gaaaaugguu aauggaaaca      420

gaagaaacgc cugauauacg ucuuguuuac uuggcauuac accacaugau gaaacaucga      480

ggccauuuuu uauuuagugg caacauugaa aaaauaaaag aauuucaaga aaccuucagg      540

caauauauag gaaaaauaag agaggaagaa cuugauuucc auuuauguau agaaggagaa      600

gaauuaagag agacagagaa cauacuaaaa gauaaaaacc uaacgcguuc ggcuaagaaa      660

acuagguuaa uaaaauuacu aggagcgcac acagcuugcg aaaaagcagc auuaaacuua      720

gucgcgggag ggacagugaa gcuaagcgac auauuuggaa auucagaacu agaugcuugu      780

gaaaaaccaa aacuaucauu ugcugaugcc gguuaugaug acuacgcagg aaugauugaa      840

gaugaauuag gugagcaaca uguaauuaua gaaacagcga aagccguaua ugacugguca      900

guauuagcag auauauuagg agacuaccgc ugcauuucag aagcgaaggc ugcgguuuac      960

gagaaacauc aaaaagaucu aagacauuua aaagaauuag uaaaagaaaa ccuaggaaga     1020

gauguauaua aggaagucuu cgucaagacu aacgaaaagc uuccgaauua cagugccuau     1080

auagguauga caaaaaaaaa cggaguaaaa agugaaaugg aaggaaagag gugugacaga     1140

aaagcauucu augauuaccu aaaaaaaaca gugguaaacg ccauacccga ugaaaguaaa     1200

acugaauauc uaagaaagga aauggaaaca gaaacauuuc uaccaaggca gguaaccaag     1260

gauaacggag uaauaccgca ccaaguccac cuccaagagu uagacgcgau acuagaaaac     1320

cuaagcggua gaauaccggc acucaaagaa aauggcagca aaauaagaga cauauucacu     1380

uuuagaauac cauauuaugu aggaccacua aaugggauug uaaaaggugg cgaaaggaca     1440

aacuggguua ggagaaaaaa agcgggacgc auauguccau ggaacuuuga ugaaauggua     1500

gacacgggag cgucagcaga agaguuuaua agaagaauga caucaaaaug uacauaccuc     1560

auccacgaag augucuuacc caaaaauuca augcuguauu cuaaauuuau gguauugaau     1620

gaauuaaaca augugcggcu aaacggugaa cccauuagug uagaauugaa acaaaaaaca     1680

uaugaagacc ucuuucagag acauagaaaa guaacacgua gacgacuaac cgauuauaua     1740

agaagagaag gaauagccgg acgagacgcu gacaucacgg gaaucgacgg ugauuuuaaa     1800

ggaagcuuaa cagcuuauca cgacuuuaaa gaaaaacuca ccggauguga auuaucacaa     1860

gcagauaaag aaaauauaau uuugaacauc acacuauuug gagaagauaa agcauuauua     1920

aaaaagagac uaggcgcuuu auauccagcu uuaacagagc cccaaaaaaa agcaauaugu     1980

gcacuaucau acaagggaug gggaaggcua agucaaaggu uauuagaagg uauaacagca     2040

ccagcaccag aaacuggaga aauauggacc guuauuagag cgauguggga aacaaaugau     2100

aauuugaugc aggugcucuc agaaaaauau uguuuugccg cugcgauaga ugaggaaaau     2160

gcaggagagg aacuaaaaga aauaacauau aaaaccguug aacaaaugaa cguaagucca     2220

gcgguaagac ggcaaauaug gcaaucauua caagugauua aagaaauaug caaaguaaug     2280

gguggcccuc ccaaaagggu guucguggaa auggcacgag agaaaaugga aucgaaaaga     2340

accgaaagua gaaagaaaag acucauugau uuauauaaaa agugccguga agaagaaagg     2400

gacuggaucg aagaauuagg aaauacagag gaaacaaggc uacgaucaga uaaauuauau     2460

cuauacuaca cucaaaaagg uagaugcaug uauucaggug aaguuauaga gcuagaagag     2520

cuuugggaua aucgaaagua ugauauugau cauauauacc cccaaagcaa aguaauggac     2580

gacagucuug auaauagggu cuuaguuaaa aaggaauaca acgcagacaa aacagaugaa     2640

uacccaauaa gagcugacau uagaggcaaa augagagcau uuuggcggau ccuucgagaa     2700

gaaggauuua uauccaaaga aaaauacaau cgauuaacaa ggggaacagg guuugaacca     2760

uccgaauugg cgggauucau agcucgacag cuaguagaaa caagacaagg uacaaaagcc     2820

guagcaucag uacucaaaca aguauuuccc gaaacggaua uaguauacgc uaaggccagg     2880

guugcuucac aauucagaca agaguuugau cuaauuaaag uccgcgaaau gaaugaucua     2940

caucacgcua aagacgcuua cgucaacaua gucguuggua acguauauua uacaaaauuu     3000

acuucgaaug cagccuggua uguaaaagaa cacccaggaa gaaguuauaa ucugaaaaaa     3060

auguuuacga gcgaaagaga cguugcaaga aacggagaaa cagcauggag ggcagggaau     3120

ucuggcacua uagcgaccgu aaaaagagua augggaaaaa auaauauauu aguuacuaga     3180

aggaguuaug aaguaaaagg aggucuauuu gaucagcaau uaaugaaaaa agggaaagga     3240

cagguaccca ucaaaggacg agaugaaaga uuagcagaua uagauaaaua cggaggauac     3300

aauaaagccg cagggacaua cuucaugcug gcugaauccg aggauaaaaa gggagcaaaa     3360

auaaggagug uugaauaugu uccacuauac uuaugcaacu gcauagaaaa agaugaagaa     3420

gcagcaaaaa aauauuuaca gaaagaaaga ggacuaaaga acccaagagu cuuaauagcc     3480

aaaauaaaaa uugauacgcu uuuuaaagua gacggauuuu auaugugguu aucaggaaga     3540

acuggaaauc aacuaauuuu uaaaggugca aaucagcuca uauuaucaga accggauaug     3600

cgaauacuua aaaaaguacu caaauacgua aauagaaaaa aagaaaauaa aaaugccgug     3660

uuaggagaac acgaccaauu accugaaaca gaccugauac ggcuauacga ugucuucuua     3720

gacaagaucg aaaauacagu uuaucauguc cgucuaucag cucaacaagg cacauuaaca     3780

aaaaauaaag acacuuuuug ugaacuauca aacgaagaua aauguauagu acuaagcgaa     3840

auccuacaua uguuucaaug ucaaucgggu agugcaaauc uuaaacuaau aaaaggaccg     3900

gguagcgcag guauacuggu acuaaauaau auaauaucga aaugcaauca aguaaguaua     3960

auacaucaaa gcccaacagg aauauacgaa caagaaauug auuuaaaaaa gaucuga        4017


<210>  5297
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5297
augaacaagg cuuacaccuu aggauuggac auaggaacca auagcguagg augggccgua       60

gugacagaug acuaccgcuu aauggcaaaa aaaaugccag uacauucaaa gauggaaaag      120

aaaaaaauaa aaaaaaauuu uuggggugcg agauuauuug augaaggcca aaccgcagaa      180

gaacguagaa acaaaagagc aacacgacga agauuacgaa gaagaaagua ccgcauucua      240

gaacuccaaa agauauuuuc cgaagaaaua uuaaaaaaag acucacauuu uuucgcaaga      300

uuagacgaau cguuccuaau cccagaagau aaacaguaug caagauuucc aauauuucca      360

acccucuuag aagaaaaagc auacuaucaa aauuauccaa cuauauacca ccuuaggcaa      420

aaacuagcgg auucaacuga aaaagcggac auacgccuag uguaucuugc uuuagcucac      480

augaucaaau accgaggaca cuuucucuuc gaaggggaac uagacaccga gaauaccucc      540

guugaagaga ccuuuaaaga auuuauagac aucuacaacg aacaauuuga agagggaaua      600

auauuuuaua aagauauacc acuaauacua acagauaaac uuagcaaauc aaaaaaagua      660

gaaaaaauuc uucaguauua ucccaaagaa aaaacgacug gaugccuagc acaauuucug      720

aaacucauug ucggcaauca aggaaauuuu aaacaagcgu uccacuuaga cgaagaagua      780

aaaauccaaa uauccaagga aacauaugaa gaagacuuag agaaacuauu aagaaaauca      840

aacgaagaaa ugauagaugu uuucuuacag guuaaaaaag uauaugaugc aauacuucua      900

agcgacauac uaagcacaaa aaugaaagau acgaaagcaa aacuauccgc aggaaugauu      960

gaaagauacc aaaaccauaa aaaagaucuu gaagaauuaa aacaauuugu ucgggcgcac     1020

cuacacgaaa aaguuacagu auucuucaaa gacagcucca aagacggaua cgccggauac     1080

aucgauggaa agaccacgca agcugacuuu uacaaauucu ugaaaaaaga auuaacagga     1140

guaccaggaa gcgaacccau guuagcuaaa aucgaucaag aaaauuuccu auuaaaacaa     1200

cggacuccca caaauggugu aauaccucau caaguacauu uaaccgaauu uaaggcgaua     1260

auagaucaac aaaaacaaua uuacccauuu cuagaaaaau cuaaggaaaa aaugauccag     1320

cuccuuaccu uccgaauacc cuacuauguu gguccacuug cacaagacaa agaaacuagu     1380

ucauuugcau ggcuugaaag aaaaacuaca gaaaagauaa aaccguggaa ugcuaaggau     1440

guaaucgacu auggugcaag ugcuacaaaa uuuauacaaa gaaugaucaa uuacgauacc     1500

uacuuaccca cagaaaaagu acuaccaaaa uauucaaugc uuuaucaaaa auacacaauc     1560

uucaacgaac uaacaaaggu ugcauacaaa gaugaucgug gaauaaaaca ucaauucagu     1620

ucugaagaaa aacuacgaau auuucaagaa uuauuuaaaa aacaacggcg aguuacaaaa     1680

aagaagcucc aacauuucuu aucagcgaau uauaacauug aagacgcuga gauucuagga     1740

guagacaaag uauucaacag cagcuacgca acauaccaug acuuucuaga acuugccaaa     1800

ccauacacgg aaaaaauaau ugaauuacua gaacaacccg aaauggaaga aauguuugaa     1860

gauauaguaa aaaucauaac aaucuucgaa gacagagaaa ugguacgaac ccaacuaaaa     1920

aaauaccaaa gaauuuuagg agaagaaauu uuuaaaaaac uaguuaaaaa gaaauacaca     1980

ggauggggca gacuuucaaa aagauugaua aauggaauuc gggaccaaaa aacaaauaaa     2040

acaauccugg acuaccucau uaacgaugac gauuuccccu auaaucggaa cagaaacuuu     2100

augcaacuua uaaaugacga ccacuuauca uuuaaagaag aaauagcaaa agaacugacc     2160

uugucagaca aacaaagccu gcuagaagua gucgaagcua uaccaggauc acccgcuauu     2220

aaaaaaggga ucuggcaaac acuuaagaua guagaggaac uaauagccau caucgguuac     2280

aaaccaaaaa acauaguaau agaaauggca cgggaaaacc aaaccacaac cggaggcaaa     2340

aauagaagca aacccagacu aaaaagucuu gaggaggccu uaaaaaauuu cgacucacag     2400

cuguuaaaag aaagacccgu cgacaaccaa ucacuacaaa aggaccgauu auaccuguau     2460

uaccuacaaa auggcaagga cauguauacu ggcgaaucac uugacaucga ccgcuuaucg     2520

gaauacgaua uagaccacau aauacccaga agcuucauug uagaucauuc acuagacaau     2580

aaaguacuag uauccucuaa agaaaaccgc uuaaaaaaag acgacguacc ggauucaaaa     2640

guagucaaaa gaaugaaagc guacugggaa aaacuacuac gagcaaacuu aaucagugaa     2700

agaaaauucu ccuauuuaac aaaacuagaa cuaacagaug acgauaaagc acgguucaua     2760

cagagacaac ucguagaaac cagacagaua accaagcaug uagcagcuau ucuacaucaa     2820

uauuucaauc agacucaaga acuagaaaaa gaaaaggaca ucagaauaau aacccuaaaa     2880

uccagucuug uuagccaauu ucgacaagua uuugguauac acaaaguuag agagauaaac     2940

caucaucauc acgcgcauga ugcuuaucua aaugccgucg ucgcucuggc auuacuaaaa     3000

aaauauccaa gacuagcacc agaauuugua uacggaucgu uugccaaauu ccacuuagua     3060

aaagaaaaca aagcaacugc caaaaaagaa uucuauucaa acauccuuaa auuuuuugaa     3120

aaagaagaac aauucugcga cgaaaacgga gaaaucuucu gggacaaaag aaaacacaua     3180

cagcaaauua aaaagguaau cuccagucau caaguaaaca ucguaaagaa aguagaagua     3240

cagacagggu cauucuacaa ggaaacaguu aacacaaaag aaaaacccga caaacuaaua     3300

aaacgaaaaa auaacuggga uguaacaaaa uauggaggau ucgggucacc ggucgucgca     3360

uacgcaguag uauucacaua cgaaaaaggu aaaaaccaua aaaaagcuaa agcaauagaa     3420

ggaauaacaa uaauggagca agcgcuauuc gaaaaagacc ccauaucauu ccuaaucgaa     3480

aaaggauacu cgaauguaaa ccaauucauu aaacucccua aauacacccu auucgaguua     3540

gcaaacggcc aacgacgaau gcuugcauca caccaagaac uacaaaaagc caacucguuu     3600

auucuaccag aaaaacuggu aacucuacua uaucaugcaa accacuauga ugagauugca     3660

uacaaagacu cauacgacua uguaaacgaa cacuucucaa acuuccagga cauccucgac     3720

aaagucauaa uauuugcaga gaaauauaca agcgcgcccc aaaaacuuaa ccagauaauu     3780

gcaacuuaug aaaaaaacca agaagccgac cgcaagauua uggcacauag cuucguaaau     3840

cuaaugcaau uuaaugcauu gggcgcucca gcagacuuca aauucuuuga cacuacaauc     3900

acucgcaaac gauacacauc gcuaacugaa auauggcaaa guacaauaau auaccaauca     3960

guaacagggc uuuacgaaac cagacguaga auggccgacu uaugggaugg cguacaguga     4020


<210>  5298
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5298
auggaaagau accacaucgg auuagacauu ggaacuucaa guauugggug ggcagucaua       60

ggugacgauu ucaagauuaa aaaaaaaaag gguaaaaauu uaauaggaac cagacucuuc      120

aaugaaggag caaccgcagc agaaagacga ggauuuagaa cacaaagaag aagauuaaac      180

cgacgaaaau ggagauuaaa auuauuagaa gaaauauuug aucccuacau ggcagaagua      240

gaugaauacu ucuucgcaag acucaaauca ucgaauuuau caccaaaaga caguaacaag      300

aaauaccugg ggagccuacu auuccccgac aaauccgaua gcaacuucua ugauaaauac      360

ccuaccauuu accaucuuag aagagacuug auggaaaaag augaaaaguu cgaucuaaga      420

gaaauauauu uagcuaucca ucacauagua aaauacagag gaaacuuccu cgaaaaagua      480

ccagcaaaaa acuauaaaaa cucaggagcc ucaaucggau uccuacucga ggaaguaaau      540

agucuuuaua aagauauaau aggagacgaa uccauagcaa ucuuaaacuc agaaaaauuc      600

gaagacguug aaaaaauaau auuagaugaa gaaaccagaa aucuagacaa acaaaaauca      660

guaggaaaac uacuaguaga agacaaaaaa aaaaaaaaua uaguuacagc auucucaaaa      720

gcaauuuucg gcuacaaguu uaacauagag gaccuucugc uaaucgaaaa ugacgaaaag      780

aacaaacuaa ccuuuaauga cgaaaacaua gaugacaucu uuaaugaguu gagucauuca      840

cuagacgaca aacaaaugga ucuccuaaca aaaacccgag aaauauacuu caaauucaaa      900

cuaaauauga uagugccgac aggcuauacc cuaagcgaaa gcaugaucga aaaauacgaa      960

augcauaaag cacauuuaaa aauguacaaa gaauucauca auacauuaaa uacaaaagac     1020

agaaagauau uaaaaaaugc auacucagau uacauaaaca cagaaaaagc caaagcagca     1080

aacgcccaag aaaauuuuua caagacaguc aagaagauaa ucaaagauaa caauagcgau     1140

acagcaaaaa aaauaauugu acuaauagau gagggaaacu ucaugcccaa acaaagaaca     1200

ggagaaaaug gcguaauccc acaucaacua caccaaauag aacuagauag aaucauagaa     1260

aaucaagcaa aauacuaccc uuggcucgca gaagccaauc cuguggaaaa aaacagaaaa     1320

uucgcaaaau acaaacuaga cgaauuagua acauuuagag uccccuacua cguaggacca     1380

cuaauugaca aaaccgaaag uaauaaaaac gaaaaagaaa caaaauucgc guggauggug     1440

cgcaaagcaa aagguacaau uacaccgugg aacuucgaaa aucuuguaga cagaacagaa     1500

ucagcaaaca gauuuaucaa aagaaugaca ucaaaagaca cuuauauaau uggcgaagau     1560

guuuugcccg caucaucauu gcucuacgaa aaauucaaag uacuuaacga acuaaauaac     1620

aucaaaguua acgaaaaaaa acuagacaua gaacagaaac aggauaucua ccuaaaccug     1680

uucacaacag caaaaaacgu aacuaaaaag ucauuagcua ccuauuuaaa uuguuccgcu     1740

gacucaauca gcggucuauc ugauggagaa aaauuuaaca gcucccuauc aucuuacauc     1800

gaucuaaaag cuauacuggg caacaucguc gaugauugua acaaaaacga ggaucuagaa     1860

aaaauuauag aauacucaac uguuuucgaa gaugggaaca ucuacaaaga aaaauuaucu     1920

gaaauaucau ggcuaacgga ugaacaaauc gagaaacuua guaauauaca uuuuaaagga     1980

uggggccgcc uuucaaagaa auuacucacu caaauaacaa augaaaacgg agaaagaauc     2040

auagaugcau uauggaacac cagcaauaac uucaugcaaa ucauaaauga ugaaagcaua     2100

caagcaaagc uagcggagau aaacggcaaa uaugccggaa aaucugaucu cgaggacaua     2160

cuuucugaag cauauacuuc gccccaaaac aaaaaggcaa uaagacaagu aaugaaaguu     2220

gucgaagaua uugaaaaagc aaugcaaugc gaaccgacau caauaagcau ugaauuuaca     2280

agaaaaaaac aaaaaaguag acuaacaaau accagguaca agaaaauauc cgacgucuac     2340

gaaaaaauag cugaugaagu gauuagcgag uacgaacuga gaaaacuaca aucggaacuu     2400

gacagcaaug caaacaauau gagagaccga uauuauuuau acuucaugca guuaggccgu     2460

gacauguaca ccggcgaaaa aaucaacauu gacgaacucc accaacguua ugacauugau     2520

cauauucucc cucaaucauu cauaaaagau gauucaauua acaauagagu acuaacaaga     2580

aaagacguca acaauaaaga aaaaucagac aaaacagccg cugacuuaua cgcaguagaa     2640

augucagauu uuuggagaaa auuaagaaaa caaggacuaa ucacagagca aaaauacaaa     2700

aauuuauuaa cuaagagcga caguauagac aaauaugcga aaaaaggguu uauaaaacga     2760

caacuaguag aaacaucaca aguagugaag cuaguagcca acauacuuca agacaaauau     2820

ucagacacua aaauaauaga aguaagagcu aaacugaacu caaaucuucg agaggaguau     2880

aaauuaauaa aaaaccgggc gguaaacgac uaccaccacg cgauagacgg uuaccugaca     2940

acauuuauag gacaauaccu cuacaaaaca uacccaaaac uaagaagcua uuucgucuau     3000

aaugacuuca aaaaauuaga cagcaacuau uugaaacaca ugaagaaauu caauuuccua     3060

uggcaacucc aggaugacaa aaccaucgac auauacgaca acguaaauaa caaauucgua     3120

cugaauguac ccgaaaugaa aaaauauaua cagaaaaucu acaacuacaa auacauguua     3180

gucucaaaag aggucacuac caaaaacgga gcguuuuaug aucaaaccaa guacaacgca     3240

aaaacuguca aucucauacc aaucaaaaaa aacaaaccaa caaauauaua uggaggauau     3300

aaaggaaaag ucucuagcua uaugaugcua guaaaaauac gaaaaaaaaa ggaaguaaua     3360

uacaaauucg uuggaguccc cagacugugg acagaugaau uagauagacu gaaggacaca     3420

acagaaagaa aaauacuauu aaacgagauc gcaaaaaauu cacuaucuaa aacagaacaa     3480

gauuucgaag uuauauuaga caaaguauau uaugggcagc uaauaauuga cggaaaccaa     3540

aaauauacac ucgggaguag ugaauauaaa uauaacgcaa ugcaacuaca uuuaagcaaa     3600

cacgcacuag aaguauuggc aaaagaaaac guaaaagaug cagaaauaac agacaaagac     3660

uuagucucag uauacgaaga aauucucuca guaguaaaca aauacuucga gcuauaugac     3720

auaaauaaau uccgacaaaa guuaaacgag ggauuggaaa uauucagaaa acuccccguc     3780

uacaacguau augaaucaaa caaaauuaaa caaguuggaa aauucgaagu acuuaaucga     3840

auauuaaugg gccuacacgc gaacgcaaug auaacggacc ucaaaguccu aggaaucaag     3900

acaaaacuag gucaaaugca aguaaacggu ggcaucaaac uaagcccaga ugcaaaacug     3960

aucuaucagu caccuacagg uaucuucagu agagccgucc gagucaaaga ccuaggauga     4020


<210>  5299
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5299
augagcuuaa auacgaacaa gaaauacaau auuggucuag acauagguac aaccucaguc       60

ggaugggccg ucacggaugc agauggacaa aaacugcuua aagcuaaaaa caaaaauuua      120

uggggaguau cacuauucga agcagguaaa acagcagcag aaagaagagg auauagauca      180

acuagaagga ggcuaaacca uagaaaauuu agacuacgcc uacuugaaga cauguuugaa      240

aaagaaauau uaucuaaaga cccuucauuc uuuauaagac uuaaagaggc uuuucuaagu      300

ccuaaagaug aacaaaaaca auucaaaggc aacauacuau uuaaugacaa agauuacacu      360

gaugccgacu acuaugaaca auauaaaaca auuuaucacc uacgcuauga cuuaauauca      420

caacaccgac aauuugacau acgagaagua uacuuagcca uacaccaucu gauuaaauac      480

agaggacacu ucauuuacga agaucaaacu uuuacaacag acggcaacca acuacaacau      540

cacauaaaag caauaauaac caugaucaau uccauccuua acaggauaau aauacccgaa      600

acaauagaca ucaacguauu ugagaaaacu uuacucgaca gaacuaugaa ucgcucaucc      660

aaagucaaau ucuuaaucga acuaacagga gaagauaaac aagacaaacc ucuuuuaaaa      720

gaacuauuua acuuaaucgu uggucuaaaa gccaaaccag cuucgauauu cgaacaagaa      780

aauuuagcga ccaucguuga aacaaugaau augucaaccg aacaaguaca auuagauuua      840

cuuaccuuag cugacgugcu agcugacgaa gaauacgauc uucuucuaac agcccagaaa      900

auauauucag ccauaauacu ugaugaaucc auggauggau acgaauauuu cgccgaagcc      960

aagaaagaau cauacagaaa acaucaagaa gaguuaguau uagucaagaa aaugcuaaaa     1020

ucgaaugcaa ucacgaauga ugaaagagca aaauuugaau auuuuuacac agauuacauc     1080

ggagcuaaaa guucaaacua ugaagaauca aaaauaauca agaaaggccu cagugccgca     1140

uacggaaaau acucaaaaga agaaagacua uucaaacaca ucgaauuauu auuagccaaa     1200

gaaaauguac uaaccacagu cgaacaugac uuacuggaga aaaacauaac cuuugcuucc     1260

uuacuccccu uacagagguc cucccgaaac gcuguuauac cguaucaagu ucaugaaaaa     1320

gaacuugucg caauucuaga aaaucaagca acguacuacc cauuuuuacu cgaacagaaa     1380

gacaauauac acaaauuacu aacauuccga auacccuacu acgucggacc acuugcagac     1440

caaaaagacu cagaauuugc guggauggug agaaaacaag ccggaaaaau aacaccauuu     1500

aauuuugaag aaaugguaga cauugaugcc agcucggaag cauuuauaaa aagaaugaca     1560

aacaaaugca ccuauuugau acacgaagac guuaucccua aacacucauu uucauaugca     1620

aaauucgaag ugcucaacga acuaaacaaa auacgccuag acgguaaacc aauugacaua     1680

ccacuaaaga aaagaauuuu ugaaggccuc uuccuugaga aaacgaaagu uacccacaca     1740

ucacuaaaga aauggcuagc ggaauaugaa cauaugaccg ugucaguugu acaggggaca     1800

caaaaagaaa ccgaauuugc uacaucgcuc caagcauuuc accgauucgu uaaaauauuu     1860

gaccgggaaa ccguauccaa cccugccaac gaagaaaugu uugagaaaau uauauacugg     1920

ucgacuguau uugaagauaa gaaaauaaug agaagaaaau uaucagaaua cccccaacuc     1980

acagaacaac aacagguuca acuagcccaa guucgauucc gaggaugggg gagacuuagc     2040

caaagacuaa uuaaccgaau uaaaacacca guaaguggag acgaagacca caaauuaagc     2100

aucaaugaaa uauuauggca aacaaacgaa aauuucaugc aaauaauuag aaacaaagac     2160

uauuuauuua agaaaauuau cgaagaacaa uuugaaaaug aaacugcguu acucaacaaa     2220

caaagaauag acgaacuagc aacgaguccc gcaaacaaga aaggaauaug gcaagcuauc     2280

aaaauuguua aagaacucga aaaagucuua cagcaaccag ccgaaaacau auucauugag     2340

uucgcaagau ccgaugaaga aucaaaacgc ucaacaccca gagacaaauu cauagaaaaa     2400

gcauacgcuc aacucaagaa agaaacggac acuuucaacu uagaacaucu uaaagaacua     2460

aaacaaagaa gcaaacagcu aagcagccaa cgacuuuucc ucuacuucau acaaaaugga     2520

aaaugcaugu acaguggcga acaccucgac auagaaagau uagacucaua cgaagucgau     2580

cauauacuuc cucaguccua uaucaaagau aacuccauag aaaacuuagc ucuagucaag     2640

aaaguagaaa aucaaaggaa gaaagacagc uuacuccuca auucgucaau aauaaaucaa     2700

aauuacucaa gaugggaaca acucaaaaac gcaggauuaa uaggagaaaa gaaauucaga     2760

aaucuaacaa ggacaaaaau aacagacaga gacaaagaag gauucauagc acgacagcua     2820

guagaaacaa ggcaaauaac aaaacaugua acacaauuac uucagcaaga guacaaagau     2880

acaacaaaag uauucgcaau aaaagcaacg cuagucucag aucuuagaag gaaauucgag     2940

uucaucaaaa aucguaaugu caaugauuau caucaugcac aagacgccuu cuuaguggca     3000

uuucucggua caaauauaac aaguaacuac ccgaaaauag aaauggaaua ucuauuuaaa     3060

ggguaccaac acuaccucaa ugaaguaggu aaauccgcug cuaaaccaaa auuuacuuuu     3120

auaguagaaa accuauccaa acaacaauau aacaguacua caggagaagu uaaauggaac     3180

ccagaaguag acauagcaaa acuuaaaagg aucuuaaauu ucaaacaaug caauauaguc     3240

cgaaaagucg aggaacaaag cggagcauua uuuaaagaaa ccauuuaucc agucgaagaa     3300

ucuagcagca agacaauccc ucucaagaaa caucuagaca cagcuaucua uggaggcuac     3360

accgccguua auuaugcauc auaugcacua auccaguuca agaaaggacg caaacuaaaa     3420

cgagagauaa uagguauacc ucuagccgua caaacccgaa uagauaauuc agaaacauca     3480

cuacaaacau acaucgcaga acaaaucaaa uccgagguag aaauauuaaa uggucgcauu     3540

cuaaaguacc aacuuaucuc uaacaacgga aacagauuau acauagcagg cccucgagaa     3600

agacacaacg caagacaacu cauuguauca gaugaagcag cgaaaguaau cuggcucaua     3660

ucaaccaaac aagcagugga ggccacuuuc cuaaaacacu auacucuaga acauuuagaa     3720

gcaguuuucg aagaauuaau ccgcaaacaa gcugcagauu accaaauauu cgaaaaccuc     3780

aucaagaaaa uugaggucaa uaaagcauau uuuuauucau guaccuacaa ugaaaaagua     3840

aaagugauag aagaacuacu aaagaucaca caagccaacg caaccaaugg cgaccugaaa     3900

uuacuuaaaa ugacuaaccg agaaggacga cuagggagcg uuucaguugc ccucgauuuc     3960

aaaaucauca aucagucggu uacagguuua uaucaaucaa uagaggacua caacaacuga     4020


<210>  5300
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5300
augucacuua auaccaauaa gaaguauaac auaggacuag auauaggaac aacgucugua       60

ggaugggccg ucacugacgc agacggccaa aaacuacuaa aagcaaaaaa caaaaacuug      120

uggggaguaa gucuuuucga agcugguaaa accgcagcug aaaggagggg cuaucggagc      180

acacgaagac gauuaaacca ccguaaauuu agacuccgac uacuagaaga cauguucgaa      240

aaagaaaucu uaucgaaaga cccuuccuuc uucauuagau ugaaggaagc auuuuuauca      300

cccaaggaug aacaaaagca guuuaaagga aacauauuau uuaacgauaa agacuauacg      360

gaugcagacu auuacgaaca auacaagaca aucuaccacc ugagauauga ccucaucuca      420

caacacagac aauuugacau ccgagaggua uacuuagcca uccaccaccu aauaaaauau      480

cgaggucacu ucaucuauga ggaccagaca uucacaacgg augguaacca auugcaacac      540

cauauuaaag caauuaucac uaugaucaac ucgauacuca aucgaauuau aauaccugaa      600

acuauagaua ucaauguuuu cgaaaaaacc uuguuagaca gaacuaugaa uagaagcagu      660

aaaguaaaau uucucauuga auuaaccggc gaagauaaac aggacaagcc uuuacuaaag      720

gaauuauuca aucucauagu aggauuaaaa gcuaagccag cuaguauauu ugaacaagaa      780

aaccuagcga ccauaguaga aacaaugaac augaguaccg agcaaguaca auuagauuua      840

uuaacacucg cagacguacu cgcagacgaa gaauaugacu uacuccuaac cgcacaaaaa      900

auauacucgg caauaauccu ggacgaauca auggacgguu augaauacuu ugcagaagcc      960

aaaaaggaau cuuacagaaa acaccaagaa gaauuaguac ucguaaagaa aaugcuaaaa     1020

ucuaaugcua ucaccaauga cgaaagggcu aaguucgagu acuucuauac agacuacaua     1080

ggagcaaaau cuagcaauua cgaagaaagu aaaauaauca aaaaaggucu aagugcugca     1140

uauggaaaau auucaaaaga agaacgacuu uucaaacaca uugaauuauu acuggccaaa     1200

gaaaacguac uaacaacagu agaacacgac uuauuggaga aaaacauuac uuuugcaucc     1260

cuacuucccu uacaacgcuc aucuagaaac gcuguaauuc cauaccaagu acaugaaaaa     1320

gaauuaguug cgauccucga aaaccaagca acauacuauc ccuuccuccu agaacagaag     1380

gacaauauac acaagcuauu aacuuuucga auucccuacu auguuggucc auuagcagac     1440

caaaaagauu ccgaauuugc uuggaugguu agaaaacaag caggaaaaau aaccccuuuc     1500

aauuuugaag agaugguaga uauagaugcc ucuuccgaag cauuuauaaa acgaaugacc     1560

aacaaaugua cauacuuaau acacgaagau guaaucccca aacacagcuu cucauaugca     1620

aaauuugaag ugcuaaauga acucaacaaa auacgccuag auggaaaacc aaucgacauu     1680

ccucugaaaa aaagaauauu ugagggacuu uuuuuagaga aaacaaaagu aacucauacc     1740

agucuaaaaa aauggcuagc ugagcacgaa cauaugaccg ucucuguagu acaaggaaca     1800

caaaaagaaa cugaauucgc uacaagccua caggcuuuuc acagguucgu aaaaaucuuu     1860

gacagagaaa cagucucaaa cccagcaaac gaggaaaugu ucgaaaaaau cauauauugg     1920

ucaacaguau ucgaagauaa aaaaauuaug agaagaaaau uaucagaaua uccacaacuc     1980

accgagcaac aacaaguaca auuagcacaa guaagguuua gaggaugggg acgucuaagu     2040

caaagauuaa uaaacagaau aaaaaccccg guuuccggug acgaagauca uaagcuaagc     2100

auaaaugaaa uuuuauggca gaccaacgaa aacuuuaugc aaaucauaag gaauaaagac     2160

uaccuauuua aaaagauuau cgaagaacag uuugaaaacg aaacugcucu acucaacaag     2220

caaagaauag augagcuagc aacaucacca gcgaauaaaa aagguaucug gcaagcaaua     2280

aaaauuguaa aagaacuaga aaaaguccuc caacagcccg ccgaaaacau auuuauagaa     2340

uucgcaagau cugaugaaga aagcaaacga uccacaccaa gggauaaauu uaucgaaaaa     2400

gccuaugcac aauuaaaaaa agaaacagau acauuuaauc uugagcaccu aaaagaauua     2460

aaacaacgau ccaaacaacu uuccucacaa agacucuuuc ucuauuucau acaaaacgga     2520

aaguguaugu auucaggcga acauuuagau auagaaagau uagauaguua cgaaguagac     2580

cacauacucc cccaaucaua cauuaaagac aauucaaucg aaaaucuagc auuagucaaa     2640

aagguggaaa accaaagaaa aaaagacagu cuacuacuaa acucgaguau aaucaaccaa     2700

aauuacucca gaugggaaca auuaaagaac gcaggacuaa uaggagaaaa aaaauucaga     2760

aaucucacuc guacaaaaau aacggaucga gacaaagaag gcuuuauagc acgacaauua     2820

gucgaaacuc gacaaauaac aaaacacguc acacaacugc uacaacaaga auacaaagac     2880

acgacaaaag uauucgcaau aaaagcaacu cuagucucug accuucgaag aaaauucgaa     2940

uucauaaaaa aucgcaaugu caacgacuac caucaugcac aagaugccuu ccucguugcu     3000

uuucucggca caaauauaac aagcaacuau ccuaaaauag agauggaaua uuuauuuaaa     3060

ggauaucaac auuaucuaaa cgaagucggu aaaagcgcag ccaaaccuaa auuuaccuuu     3120

auagucgaaa aucuauccaa acaacaauac aauaguacca ccggagaagu aaaauggaac     3180

ccugaaguag acauagcuaa acuaaaacgu auauuaaacu ucaaacagug uaacauagua     3240

agaaaaguag aagaacaauc cggugcacua uuuaaagaaa cgauauacuc gguagaagag     3300

uccucaagca agaccauccc acuaaagaaa caccucgaca cugcuauaua cggaggauau     3360

acagcaguaa auuaugcauc cuaugcauug auacaauuca aaaaaggcag aaaauugaaa     3420

agagagauaa ucggaauccc ccucgcggua caaacaagga uagacaauuc agaaacaucu     3480

uuacaaacuu acauagccga acaaaucaaa ucugaaguug aaauucucaa cggacgaauc     3540

uuaaaauauc aacuaauuag caauaauggg aaucgauuau acauugcagg accaagggaa     3600

agacacaaug cuagacaauu aaucguaagu gacgaagcag cuaaaguaau auggcuuaua     3660

ucaacaaaac aagcaguaga ggcaacauuu uuaaagcauu acacacuaga acauuuagaa     3720

gcgguguuug aagaacuaau aagaaagcag gcagcagauu aucagauauu cgaaaacuua     3780

aucaaaaaaa ucgaaguaaa uaaagcguau uuuuauaguu guacauauaa ugaaaagguc     3840

aaaguaauag aagaauuacu aaaaauaacu caagcaaacg caaccaacgg agauuuaaag     3900

cuccuuaaaa ugacuaacag agaaggccgc cuaggcagug ucucaguugc auuagauuuu     3960

aagauaauaa aucaaucugu uaccggacua uaccaaucaa uagaagacua caacaacuga     4020


<210>  5301
<211>  4020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5301
augaaaaaag aauacacuau aggacuugau aucgguacca auagcguugg cugguccguc       60

uugacagaug acuacagauu aguaucgaaa aaaaugaaag uagcuggaaa uacagaaaag      120

ucaaguacaa aaaaaaauuu uuggggcgua cgacucuuug augaagggca aacagcagag      180

gcacgaagau caaaacgaac cgcgagaagg agacuagcca gacgaagaca aagaauacuc      240

gaauuacaaa aaauauucgc accagaaauc cuaaagauag augaacauuu cuucgcaaga      300

cucaaugaga gcuuuuuagu uuuagaugaa aaaaaacaau cucgccaccc aguauucgca      360

acuauuaaac aagaaaaauc auaucaucaa acauacccca cuauauauca ccuacgccag      420

gcacucgcug acaguucuga aaaagcagau auuagacuag uauacuuagc aauggcccac      480

cuccucaaau accgagguca uuuccuaaua gaaggugaac uaaacacaga aaacucaagc      540

guuacugaaa ccuuuaggca auuucuaucg acauacaauc aacaauuuuc cgaggcagau      600

gauaaacaaa ccgaaaaacu ggacgaagcu guggacugua guuuugucuu uacagaaaaa      660

augucaaaga caaagaaagc agaaacccua cuaaaauacu ucccucacga aaaauccaac      720

ggauaucuuu cacaauucau aaaacuaaug guaggaaacc aagguaauuu caaaaacgua      780

uuuggacuug aagaagcaaa guugcaauuc agcaaagaaa cauaugaaga agaucuagaa      840

gaacuacuag aaaaaauagg agacgauuau auugaccucu ucguucaagc aaaaaacguc      900

uacgacgccg uguuacucag ugagauacuu ucagauucga cgaaaaacac aagagcaaaa      960

uuaagugcag ggaugauaag aagauacgac gcacacaagg aagaucucgu acuacugaaa     1020

agauucguaa aagaaaaccu accaaaaaag uacagagcgu uuuucgguga caacaguguu     1080

aacggcuacg caggcuacau agaaggccac gcaacccaag aagacuuuua caaguuugua     1140

aaaaaagaac ucacaggaau aagaggaucc gaaguauucc uaacaaaaau ugaacaagag     1200

aacuuuuuaa gaaaacagcg uacauuugac aaugguguaa uaccacauca aauacaccug     1260

acagaauuaa gggcaauaau agcaaaucaa aaaaaacauu acccauuucu aaaagaagaa     1320

caagaaaaau uagaaaguuu guuaacauuu aaaauccccu auuacgucgg accgcuggcc     1380

aaaaaacaag aaaauucucc auucgcaugg cuaauacgaa aaucggaaga aaaaauaaaa     1440

cccuggaacc uccccgaaau aguagauaug gaaggaagcg cgguacgauu cauagagaga     1500

augauaaaua cggacaugua caugccccau aacaaaguac ucccuaagaa cagucuauua     1560

uaucaaaaau uuaguauuua caaugaauua acaaagguac gauaccaaga ugaacgaggc     1620

caaaugaacu acuuuuccuc aauagagaaa aaggaaaucu uccacgaauu auucgaaaaa     1680

aauagaaaag uaacaaagaa agaccuacag gaguuucucu accucaaaua ugacauaaag     1740

cacgcagaau ugagcgguau agaaaaagcu uucaacgcga gcuacacaac cuaucaugac     1800

uuucucacua uguccgaaaa caaaagagaa augaaacaau gguuagaaga ucccgaacuu     1860

gcaucgaugu uugaggaaau cauaaaaacg cugacugucu ucgaagauag agaaaugauu     1920

aaaacuagau uaagccauca cgaagcaaca cugggaaaac auaucaucaa gaagcuaacc     1980

aaaaaacauu auacaggaug gggacgucuc aguaaagaau uaauacaagg uauaagagau     2040

aaacaaagca auaaaacaau ucucgauuac uuaauaaacg augacgacuu cccacaucau     2100

agaaauagaa auuucaugca acugauaaac gacgacaguu uaucauucaa aaaagaaaua     2160

aaaaaagcac aaaugauuac ugacacagaa aacuuagaag aaauaguaaa ggaauuaacu     2220

ggcucacccg caauuaagaa agguaucuua caaagucuaa agaucguuga ugaaaucgua     2280

ggcaucaugg gauaugagcc agccaauauc guaguagaaa uggccagaga aaaccagaca     2340

acaggaaggg gacuuaaauc aucaagaccc cgacuuaagg ccuuagaaga aucuuugaag     2400

gauuuuggau cccaacugcu uaaagaauac ccaacagaca auuccucauu acaaaaagau     2460

agacuuuacu uauauuauuu acaaaauggg cgcgacaugu auaccggagc cccacuagau     2520

auccaucgcu uaagcgauua ugacauagac cacauuauac cacgaucuuu cacaacagac     2580

aauuccaucg auaacaaagu acuuguaucu agcaaagaaa acagacuaaa gaaagacgac     2640

guuccaucag aaaagguagu aaaaaaaaug cgaucguucu gguacgaccu auauucaagc     2700

aaacuaauau ccaagagaaa auuagacaac uuaacaaaaa ucaaauuaac ugaagaagac     2760

aaagcaggcu ucauaaaaag acagcuugua gaaacgagac aaauaacaaa acacguagca     2820

ggaauauuac accaccgauu uaauaaagcu gaagacacua augaaccaau aagaaagguc     2880

agaauaauaa cacuaaaauc agcacucgua agccaauucc gcaauagauu uggaaucuac     2940

aaaguaagag agauaaauga auaccaccac gcacacgacg ccuacuuaaa cgguguagua     3000

gcauuagccc uucuaaaaaa auacccccaa cuagccccgg aguucgucua cggagaauau     3060

uuaaaauuca acgcacauaa agcaaacaaa gccacgguua aaaaagaauu cuauaguaau     3120

aucaugaaau uuuuugaauc cgacacacca gugugugacg aaaauggcga gauauucugg     3180

gacaaaagua aaucaauagc gcaaguuaaa aaaguaauaa accaccacca uaugaacauc     3240

guaaagaaaa cagagaucca aaaaggcggg uuuucaaagg aaacaguaga gccaaaaaaa     3300

gacucaucaa aacuguuacc aaggaaaaau aauugggacc cugccaaaua uggaggguug     3360

ggguccccua acguagcuua cacgguugcu uuuacauacg aaaaaggaaa agcaagaaaa     3420

cggacuaaug cccuagaagg aaucacgauc auggagagag aagcuuuuga acaaagccca     3480

guacuuuucc uuaaaaacaa aggauaugaa caagcggaaa uugaaaugaa acucccuaag     3540

uacgcgcuau ucgaacugga aaacgguaga aaaaggaugg uagcaucaaa uaaagaggcu     3600

caaaaggcca acucauuucu acuaccagaa cacuuaguaa cacuacuaua ccacgcuaaa     3660

caauacgacg agauuucuca caaagaaagu uuugauuaug uaaaugaaca ccacaaagaa     3720

uuuagugaag uauuugcaag aguacuagaa uuugccggga aauacacccu agcagaaaaa     3780

aacauagaaa aauuggaaaa aauauauaaa gaaaaucaaa cagaugaccu agcaaaacua     3840

gcgucaagcu uuguuaaucu aaugcaauuc aaugcaaugg gagccccugc ggacuuuaaa     3900

uucuuugaug ucacaauacc cagaaaaaga uauacaaguc ucacagaaau auggcaauca     3960

accauuauac aucagucaau aacaggucuu uacgaaacgc gaaucagaau gggaaaauga     4020


<210>  5302
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5302
augaaaaaag aauauacuau cggauuagac auaggcacua auuccgucgg uuggagcguc       60

uuaaccgaug auuauagacu agucaguaaa aaaaugaaag uagcuggaaa uacagagaaa      120

agcagcacua aaaaaaacuu uugggggguu agacuauucg acgaaggaca gacugcagaa      180

gcgcgacguu caaaacguac ggccagaaga agauuagcaa gaagaaggca gagaauuuua      240

gaacugcaaa aaaucuucgc accagaaaua cuaaaaauag augaacauuu cuuugcaaga      300

cuuaacgaaa gcuuccucgu ccuugaugaa aaaaaacaaa guagacaucc aguauucgca      360

accauaaagc aagagaaaag uuaccaucaa acauacccaa ccauauauca ucuacgacaa      420

gcauuagcug acucaucaga aaaggcagac auaaggcuag uauaucuagc cauggcucac      480

cugcucaaau acagggguca cuuccuuaua gaaggggaac uaaauacuga aaacucaagu      540

guaacugaaa cauuccgaca auuucuuuca acguauaacc aacaauucag cgaagcagac      600

gacaaacaaa cagagaaauu agaugaagca guggacuguu ccuucguauu uaccgaaaaa      660

augucuaaaa cgaaaaaagc cgaaaccuua cuuaaauacu ucccgcauga aaaaucuaau      720

ggguaucuau cacaguucau uaaacuaaug guagggaauc aagguaacuu caaaaacgua      780

uuuggucuag aagaggaagc aaaacuacaa uucucaaagg aaaccuacga agaagauuua      840

gaggaauuau uagaaaaaau uggagacgau uacauagauc uauucguaca ggcaaaaaau      900

guauacgacg caguucuacu uagcgaaauu cuaagcgaua guacaaaaaa uacaagagca      960

aaacugucag ccggaaugau ccgaagauau gaugcgcaca aagaagaccu aguauuacua     1020

aagagauuug ucaaagaaaa ccucccaaaa aaauacagag cuuucuucgg agauaauuca     1080

guaaacggcu acgcaggaua cauagaagga cacgccacuc aagaagauuu cuacaaauuc     1140

guaaaaaaag aacuaacagg aauaagagga ucagaaguuu uccucacaaa aauugaacaa     1200

gaaaauuuuu uaagaaaaca aagaacuuuc gauaaugggg ucauaccgca ucaaauccau     1260

cuaacugaac ucagagcuau aaucgcaaau caaaaaaaac auuauccauu ucucaaagaa     1320

gagcaagaaa aacuagaauc uuuauuaaca uuuaaaauac ccuacuacgu cggucccuua     1380

gcuaaaaagc aagaaaauuc accauuugca ugguuaauaa ggaagucaga agaaaaaauu     1440

aaacccugga accuuccaga aauaguagau auggaaggga gugcagucag auucauagaa     1500

agaaugauua auacugacau guacaugcca cacaacaaag uccuuccaaa aaacagccuc     1560

cuuuaccaaa aauucucaau cuacaaugaa uuaacuaaag uaagauacca agacgaacga     1620

ggacaaauga auuacuuuuc aagcaucgaa aaaaaagaaa uauuccacga acuguucgaa     1680

aaaaacagga aaguaacaaa aaaagaccua caggaauuuc ucuacuuaaa auacgauaua     1740

aaacaugcag aacuuucugg uauugaaaaa gcauucaaug cauccuauac gacauaccau     1800

gacuucuuaa cuaugucaga aaacaaaaga gagaugaaac agugguuaga agacccagag     1860

cuagcaucga uguuugaaga gauaauaaaa acucuaacgg ucuucgaaga uagagaaaug     1920

aucaaaacua gauuaagcca ccaugaggcc acacucggaa aacacauaau aaaaaaacua     1980

acaaaaaaac acuauacagg guggggacgc uuaaguaaag aacuaauuca aggaauacga     2040

gacaaacaga gcaacaaaac uauacucgac uaucuaauaa acgacgauga cuucccucac     2100

cauagaaauc gcaacuucau gcaacucaua aaugacgacu cauuauccuu uaaaaaagaa     2160

auuaaaaagg cacaaaugau uacagacacc gaaaaucucg aagaaauugu uaaagaauug     2220

acuggaagcc ccgcaaucaa gaaaggaaua cugcaaucac uaaaaauagu agacgaaauc     2280

guaggaauaa uggguuacga accagccaac auagucguug aaauggcuag agaaaaccaa     2340

acgaccggga gaggacuaaa aucuuccaga ccucguuuaa aagcccucga agaaaguuua     2400

aaagacuuug gaucucaacu uuuaaaagaa uauccgacgg auaauucauc uuuacagaag     2460

gaccgccuau aucucuacua uuuacaaaac ggaagagaca uguauacugg agcuccacuc     2520

gacauacaca gauuaagcga uuaugacauu gaucacauaa uaccacguag uuuuacgacg     2580

gacaauucaa uagauaauaa gguauuagug agcagcaaag aaaaccgacu aaaaaaagac     2640

gacguaccuu ccgaaaaggu agucaaaaag augagaucgu ucugguacga ucuauauagc     2700

aguaaacuaa ucagcaaaag aaaacuugac aaucucacua aaauaaaacu cacagaagaa     2760

gauaaagcug gauucaucaa aagacaacuc guggaaacaa gacaaauuac aaaacauguu     2820

gcuggaauac uacaccaccg auuuaauaaa gcagaagaca caaaugaacc cauaaggaag     2880

guucgaauca uaacacucaa gagcgcauua guuagccaau uuagaaacag auucggcauc     2940

uauaagguca gagaaauaaa cgaauaccac caugcccacg acgcguaccu caauggagua     3000

gucgcucuag cauuguuaaa aaaguauccc caauuagccc cagaauucgu guacggagaa     3060

uaccucaaau uuaacgccca caaagccaac aaagcaaccg uuaaaaaaga auucuauuca     3120

aauauaauga aauucuucga auccgauacc ccugugugcg acgaaaaugg agaaauauuc     3180

ugggacaaau cuaaaaguau ugcacaagua aaaaaaguca uaaaccauca ucacaugaau     3240

aucguaaaaa aaacagaaau acaaaaagga ggauucagua aagaaacagu cgaaccuaaa     3300

aaagacucgu ccaagcucuu accaaggaaa aauaacuggg aucccgccaa auaugguggu     3360

cuaggaucgc cuaauguggc cuauaccguu gcauuuacgu augaaaaagg aaaagcgaga     3420

aaacgcacaa augcauuaga aggcauaacu auuauggaac gagaggcuuu cgaacaauca     3480

ccaguguuau uccuaaaaaa uaaagguuau gaacaagcag aaauagaaau gaaacuaccc     3540

aaauaugcau uauuugaacu ugaaaacggu aggaaaagaa ugguagcauc aaacaaagaa     3600

gcucaaaagg cuaauaguuu ucuuuuacca gaacaucuag ucacuuugcu auaccacgcu     3660

aagcaauacg augaaauuuc acacaaggaa aguuuugauu acgugaacga gcaucacaaa     3720

gaauucagcg aaguauuugc aagaguacua gaauuugcug gaaaauacac acucgcugag     3780

aaaaacaucg aaaaauuaga gaaaauauac aaagagaacc aaacagacga uuuagccaaa     3840

cuagccagua guuuugugaa ucucaugcaa uuuaacgcaa ugggcgcucc agcagauuuu     3900

aaauucuucg auguuacuau accgagaaaa agauacacca gccugaccga aauauggcaa     3960

ucaacaauca uccaccaauc aauaacagga cuguacgaga ccagaauaag gauggguaaa     4020

uga                                                                   4023


<210>  5303
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5303
augaaaacag auuacuucau gggacuugau augggcacag guagccuagg uugggccgua       60

acugacucua gcuaucauuu augccgggcu cacgggaaag accuaugggg uguuagacuc      120

uuugaaacug cucaaacagc agaagaaaga cgaguuuucc gauguaauag aagaagauua      180

gauagaagaa aaagaaggau ccaauuacua caagacauau uugcaccaga aauauauaaa      240

aucgauccag guuucuuucu uagacucaag gaaucaagau aucuccccga agacaagaaa      300

aaucucgaag gaaaaacucc cgaauuaccg uacgcauuau uuguugacaa aaaguauaca      360

gauaaagauu uccauaaaga auacccaaca auauaccacu uaagacugag acugaugcaa      420

gauaggacac cagcugacgu cagauuagug uacuuagcau uacaccaccu aauaaaacau      480

agaggacauu ucuuauuugc uggaaucgaa gcagauaaag uaaccgacuu cagugcagcc      540

uucucagaau uccuuagaau aguucaggaa caagagcuug acauaucuuu uacaccugau      600

gaagagagca gccaaauaau aaaagaaaca cuacaaaaua cuaacuucac aagauccaag      660

aaauccacag aauuaauaaa ggggcucaaa gcaagcacug gaugugaaaa agcuuuacua      720

aaacuaauca gcggaugcaa ggucaaauua agcgcaguau ucgaugaccc uggaugcgac      780

gccuaugaga aaccacaagu cugcuuugca gaaagcucau augaagauua ugccgcuaua      840

cuagaagacg aacuugagga ccgguuuauu guaauagccg cagcaaaagc acuguaugau      900

uggagugucu ugguaaacau ucuuggagaa gcgagcacgg uaagcgaagc aaaaguaggc      960

aucuacgaaa aacacagacg agacuuaaaa uauuuaaaac auuuaguuaa aacaaaucua     1020

acaagagaag auuaccaaga uuuauucgua aaggcaggcg ccaaaaaaaa caacuacugu     1080

gcauauauug gaaugacuaa gaaaaauaac aaaaaagucg uacuagaaga aaaacgaugu     1140

acaaaagaag auuuuuacgc uuaccuuaaa aaagaaauau acaguaaagu acaagaaaau     1200

ccagaagcag aauaucuacu agaagaauua gaaaaaggaa cauuucuucc aagacagguu     1260

aguaaagaca acggaguaau cccguaccaa cuacaauucc uagaacuaca aaaaauacua     1320

aaaaaugcag uugaguauuu accguuccua aaagaagaau ccgaaaaaau ccagcaaaua     1380

augacauucc guauaccaua uuaugucggc ccauuaaaug guaaaaaaga aggucaaaau     1440

accuucgcau gggcaguuag aaaaaauaac gagccaguuu accccuggaa uuuugaagaa     1500

guuauagaua cagaagcgag cgcagaacaa uuuauuagaa gaaugacaaa uaaauguacu     1560

uauuuaccag gagaagacgu uuuaccaaaa gaaagucuau uauauucgaa auuuauggua     1620

cuaaacgaau uaaauaaccu aagaauaaau ggaacuaaga ucaguguaga acucaaacaa     1680

gagcuguacg aaaaacuauu ccagagauau agaaagguau ccgcaaaaaa auugcgugac     1740

uaccucguua gaaaugggau aacagaaaaa gaugcagaaa uuaguggcau agacggcgac     1800

uuuaaaggaa cauuaagagc auaucacgac uucaaagaaa aacuaacugg cguagaacua     1860

acggaggccg aaaaagaaac caucaucuug aauaucaccu uauucgggga agauaaagua     1920

cuauuaaaaa aaagaaugca acgacuuuua ccuaaccuca cagaggggca aaaaaaggca     1980

auaguaacau uaaacuauuc aggauggggc agauuaagca gggaauuccu cgaagaagua     2040

caagcaccaa acccugaauc cggagaaaua cucucaauaa uuagagcgcu augggaaaca     2100

aaugcaaauu uaaugcaacu gcuaucuaau caauacggau accaagaaau auuagaacaa     2160

augcgugccg auuggacagg acauaaacuu gauuaucaaa cgguagaaga acuguaugua     2220

ucacccgcag ugaagagaca aauauggcaa acccuacaag uaguuaaaga auuaaaaaaa     2280

guaaugggag gagaaccaaa aagaguuuuc uuagaaguag ccagagaaaa aauggagucu     2340

ggucgaaccg uuagccgaaa gaaacaucuu aaagaucuuu aucuaucaug caaaucggaa     2400

gaacguaacu ggcuagcaga acuugacaga caugaagagc acagcuacag aucggaccgc     2460

cuuuuccuau auuauaccca aaaaggcaga ugcauguauu gcgggaagcc aaucgaucua     2520

ggacauuuau gggacguaaa uauguaugau auagaccaca uuuauccaca aagcaaaguc     2580

auggacgaua guauaagaaa ccguguauua acuugcagaa cauguaaugc agagaagacu     2640

gaccauuacc cuuuaaaagc ugagauaaga caaaaaauga gaccauucug ggauaguuua     2700

aaagccggca acuucauaga agaagaaaaa uggaaacgau uaauuagaag ugaagaauuc     2760

accagcacug aacuugcugg uuuuaucgaa agacaacuag ucgaaacaag acaaucuacu     2820

aaagcaguug ccgauaucuu gaagcaagca augccagaaa cagaaauagu auacguaaaa     2880

gcaaaaaacg ucagugcuuu ucgacaagac uuuaacuuug uaaaaaaccg cgauguuaac     2940

gauuuccacc augcgaaaga cgcguacuua aacauugucg uuggaaauac auacuucgua     3000

aaauuuacaa aaaaugcauc uugguuuaua gaaagaaauc cagggaggag cuauaacuua     3060

aaaaggaugu ucaccaguga uuacgaugua augucaaacg gagaaauagc auggaaagca     3120

ggaaacucag gaacaaucac uacaguacgc aaaaugauga aaaaaaauag cguacuauuc     3180

acaagacguu ccuucgaggu uuccggaggu cuauuugacc aacagcuuau gaaaaaaggc     3240

aaaggacaag uauacguaaa aagcggcgau gaaagacuua gaucaauaga aaaauauggu     3300

ggguacaaua aagcuacagg ugcauauuuc augcuaguag aaagcgaagg aaaaaaagga     3360

guuaaaaaaa gaacuaucga auuuguacca cucuacagaa aagcagaauu agaagcaucu     3420

gaagaaaaaa ugcuagaaua ucuuagaacc gagauuggcu uaaaagaacc acacguacua     3480

cugcacaaaa uaaagacuga uaccuuguuu caagcugaug guuucaaaau gcacuuaucu     3540

gguagaacag gaaaacaauu aguauuuaaa ggugcaaacc aauuuuuggu agcugaagau     3600

uaugagcggu uauugaaaaa ggugggaaag gucacagaac aacuuaaaac aaauaaaaac     3660

uacaaaauua augauuacga cgguauaacu gaagaaggau uaaaagcgcu cuacucagaa     3720

uuucuaucaa aacugcaaaa agcaccauac gguaacagau uaggaaccca agcagaaaca     3780

cuagagaacg gaaaggaaaa guucgauaaa cuauccuuag aaaauaagug ccaaguauug     3840

ucagagauuu uacaccucuu ucaguguaac agcucagcag ccgaucuauc aaaacucggu     3900

ggaccaggac acggaggaau ucuuguacuc aguaacgaaa uuacaaaaug caauaaaauc     3960

aguauaauac accaaagccc agccggaaua uacgaacaag aaauagaucu gaaaacacua     4020

uga                                                                   4023


<210>  5304
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5304
auggagcaag aauauuacuu gggauuggac augggcacag gaaguguagg augggcugua       60

acagauucug aauaucaugu auuacgaaag cacgguaaag cauuaugggg agucagauua      120

uuugaaagug caucgacagc agaagaaaga agaauguuua ggacaucaag aagaaggcua      180

gaucgaagaa auuggcgaau ugaaauauua caagaaauau uugcagaaga aauaucaaag      240

aaagacccag gguuuuucuu aaggaugaaa gaauccaaau acuauccaga agauaaacga      300

gauauaaaug gaaauugccc agaguugcca uacgcucuuu ucgucgauga ugacuuuacc      360

gauaaagacu aucacaagaa auuuccaacu aucuaccacc uaagaaaaau gcuuaugaau      420

acggaagaga cgccagacau aagguuagua uaucuagcga uacaucacau gaugaaacac      480

agaggacacu uucucuuauc gggcgauaua aacgagauaa aagaguucgg aaccacauuc      540

ucaaaacuac uagaaaacau uaaaaacgaa gaauuagauu ggaaccuaga acuuggaaaa      600

gaagaauaug caguagugga aaguauauua aaagacaaua uguuaaacag aucgacgaag      660

aaaacgagac uaauaaaagc auugaaagca aaaagcaucu gugaaaaagc gguccugaau      720

uugcuggcag gaggcacagu aaaauuaagu gauauauuug gucuagaaga acuaaaugaa      780

accgaaagac caaagauaag uuucgcagac aacggcuacg augauuauau aggagaggua      840

gaaaaugaau ugggugaaca guuuuauauc auugaaacag caaaggcggu auaugauugg      900

gcaguauuag uggaaauauu aggaaaauac acaagcauaa gcgaagcaaa aguagccaca      960

uacgaaaaac auaaaucaga ucuccaauuu uugaaaaaga uaguaagaaa auauuuaaca     1020

aaggaagagu auaaggauau auuuguaagu acaucagaua aacucaaaaa cuacagugca     1080

uauauaggua ugacaaaaau uaacggaaag aaaguagacu uacaauccaa aagauguuca     1140

aaagaagagu ucuacgauuu cauaaagaag aauguucuaa agaaauuaga aggccaacca     1200

gaauacgaau auuuaaaaga agaauuagaa agagagacau ucuuaccaaa acaaguaaau     1260

agagacaaug gaguaauacc cuaucaaauu caucuauaug aacuuaagaa gauuuuaggg     1320

aaucucaggg auaagauaga ucuaauaaaa gaaaaugaag acaaacuugu gcaauuauuc     1380

gaauuuagga ucccuuacua cguaggacca uuaaacaaga uugaugacgg uaaagaaggc     1440

aaauuuacau gggcgguaag aaaaucaaac gagaagaucu auccauggaa cuucgaaaac     1500

guuguugaca uagaggcaag ugcagaaaag uuuaucagga ggaugacaaa uaagugcacu     1560

uaucugaugg gugaagaugu auuacccaaa gauagccuac uuuacagcaa auauauggua     1620

uuaaaugagc ucaacaaugu gaaauuggau ggagaaaaac ugucaguaga auuaaaacaa     1680

aggcucuaca ccgauguauu uugcaaauau cguaaaguaa caguaaagaa aauuaaaaau     1740

uauuuaaaau gugaagguau aauaucagga aaugucgaaa uaacaggaau ugauggagac     1800

uuuaaggcua gccuuacggc uuaucacgac uuuaaagaaa uauugaccgg uacugaauua     1860

gcgaaaaagg auaaagaaaa cauaauaaca aauaucguac uauuuggcga ugacaagaaa     1920

uugcuaaaga aaagacuaaa uagauuauau ccacaaauaa cacccaauca gcugaagaaa     1980

auaugugccc uaucauauac agggugggga agauuuucca agaaauuccu ugaagaaaua     2040

acugccccag auccagaaac aggggaaguu uggaauauaa uaacugcacu augggaguca     2100

aauaacaauc uaaugcaauu acuaucaaac gaguauagau ucauggaaga gguagaaacg     2160

uacaauaugg gaaaacagac aaaaacucuu ucguaugaaa ccguagaaaa cauguacgua     2220

ucaccuagug ugaaaagaca aaucuggcaa acauuaaaaa uaguaaaaga guuagaaaaa     2280

guaaugaaag agaguccaaa acgugucuuu auagaaaugg cuagagaaaa acaagaauca     2340

aaacgaaccg aaucacgaaa gaaacaauua auagaccuuu acaaagcaug caaaaaugaa     2400

gagaaagacu ggguaaaaga acucggagac caagaagaac aaaaguuaag gagugauaaa     2460

uuauaccuau auuauacaca aaaaggacga uguauguaca gcggggaagu aauugaauua     2520

aaagauuuau gggauaacac gaaauaugau aucgaucaua uauauccgca aucuaagaca     2580

auggacgaca gucuaaacaa caggguacuu guaaagaaga aauacaaugc aaccaaauca     2640

gauaaauacc cauuaaacga aaauauaaga caugaacgaa aaggauuuug gaaaagccua     2700

cucgauggag gcuuuauaag uaaagaaaaa uaugaacguc uuauacgaaa cacagagcug     2760

agucccgaag aacuagcagg auucauagaa aggcaaauug uagagacgag acagucaaca     2820

aaggccguag cggagauauu aaaacaaguu uucccagaga gugaaaucgu auacguuaaa     2880

gcagggacag uaucaagauu ucgaaaagac uuugaauuau uaaaagucag ggaaguaaac     2940

gaucuacacc augcaaaaga cgcguauuua aauauaguag uaggaaacuc auauuaugua     3000

aaauuuacca aaaaugcguc gugguucaua aaggagaauc caggaagaac auacaauuua     3060

aagaaaaugu uuacaagcgg guggaauaua gaaagaaacg gagaaguagc augggaagug     3120

gguaagaaag gaacaaucgu cacaguaaaa cagauaauga auaaaaauaa cauuuuaguu     3180

acaagacaag uacacgaggc uaaaggagga cuauuugauc aacaaaucau gaagaaagga     3240

aaaggacaaa uagcaaucaa agaaacagac gaaagauuag cauccaucga gaaguaugga     3300

ggguauaaua aagccgcggg ggcguauuuc augcuaguag aaagcaagga uaagaaagga     3360

aaaacaauaa gaacgauaga guucauaccc cuauaucuga aaaauaaaau agaaagugac     3420

gaaagcauug cuuugaacuu ccuagaaaaa ggaagaggau uaaaagagcc aaaaauucuu     3480

uuaaagaaaa uaaaaauaga uacacuauuu gauguagaug gauuuaaaau guggcuaagc     3540

ggucgaacgg gcgaucguuu auuauucaaa ugugccaauc aacuuauacu agaugagaaa     3600

auaauaguaa caaugaagaa aauuguaaaa uuuauacaaa gaaggcaaga aaacagagaa     3660

cuaaaacuua gcgauaaaga cggcauagau aaugaagugc ugauggaaau auacaacacc     3720

uucguggaca aacucgaaaa uaccguauau agaauaagac uaucagaaca agcuaaaaca     3780

cuaaucgaua aacaaaaaga auuugaaagg uugucacuag aagauaaaag caguacuuua     3840

uucgaaauuc ugcauauauu ucaaugccaa ucauccgccg ccaaccuaaa aaugauagga     3900

ggaccaggca aagcagggau auuagucaug aauaacaaca ucucgaaaug uaacaaaaua     3960

ucaauuauua accaaucacc gacagguaua uuugaaaacg aaauagaccu auuaaaaaua     4020

uga                                                                   4023


<210>  5305
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5305
augggagaag aguauuucuu aggccucgac augggaacug gaucauuagg uugggcggua       60

acagacgaua aauaccaaau ucuaagaaga cauggaaacg cucuaugggg uguacggcuu      120

uuugaaacgg caaauacagc cgaagaacga agaauguuua ggacaaaccg aagacgacua      180

gaucgacagg gguggcgaau caagauacua caagaauuau uugcugaaga aauuuguagg      240

guggacccag guuucuacuu acgcaugaaa gaaaguaaau acuacccuga agacaaacga      300

gaugcaaaug gcgaaugccc ggcacuacca uacgcuuuau ucgcagacgc acaguacaca      360

gauaaagauu aucauagaga auuucccaca auauaccauc uccgaaaaau gcugauggaa      420

acagaaacaa cacccgauau uagacuagua uaucucgccu uccaccacau gaugaaacac      480

agaggccauu ucuuauuauc uggagauauc ucgcaaguca ccgaauuccg uaacacguuu      540

uuacaaauga uagcgaacau uaaaagcgaa gaauuagacu uuucuuuaga aauagaggag      600

acccaaaugg gccaaauuga agcuguguua aaagaucaua aacuaaccag aucggaaaaa      660

aaaaggcgac ugacacaaau auuaaaaccu gccaccgcgu gcgaaaaagc aaucuuuacc      720

uuacuaucag ggggaacagu aaaacuaagc gacauauuug gaaaggcuga auuagaugaa      780

ggagaaagac ccaaaauaag cuucgcagau aacggauacg augacuauag cgccucagug      840

gaagcagauc uaggcgaaca auauuauaua auugaaucag cgaaagcagu uuacgacugg      900

ucuguauuau cagauauauu gggcgauagc guaucaauau cggaugcuaa gguaaaacua      960

uaugaaaaac acaaaaaaga uuuagcuuau cuaaaaaagg uaguaagaga aaaucugcca     1020

agagaaguau acaaucaagu cuucguaaac acugaugaaa aauuauguaa cuauugugcg     1080

uauauuggua uggcaaaaaa aaauggaagg aaaguagcac uagaaggaaa aagaugcaca     1140

agagaagagu ucgagaguuu uuuaagaaaa aaaauaguaa auaaaaucga agacaaagaa     1200

auccaagagu acuuauccga agaaaucgaa aagggaacau uccuuccaag acaagucacu     1260

aaagacaaug gaguaauacc guaccaaauu cauuuauaug aauugagaaa aauaaucgac     1320

aaccucaguc caagaaugcc uuuuauaaga gauaacggag acaaaauaau acaacuguuu     1380

accuuuagaa uaccauauua uguggguccu cuaggaaaag gagaaggaag cgaaaaauuu     1440

uccugggcgg uaagacgaac cgacgaagcg auauacccgu ggaacuuuaa agauguaaua     1500

gauguagaag ccucggcaga aaaauuuaua uguagaauga caaauaaaug cacauaccua     1560

uauggagaag acguccuccc aaaagacagu ccacuauaua gcaaauuuau gguccuaaau     1620

gaauuaaaua auuuaaaauu agauggacaa agcgucucug uggaguugaa gcaacggaua     1680

uaucaagaac uauuuugcca uacuagaaaa gugacacaaa aaaagcuagu aucuuaucua     1740

guuagagaag gaaucacugg aaaaaaugua gaaauuucag ggauagacgg agacuuuaaa     1800

gcaagccuua cagcguacca cgauuuuaaa gaaaaacuua caggaguaga auuaagccag     1860

caagaacaag aagaaauagu ucgaaauauc guacuuuuug gugaagacaa aaaacuuuua     1920

aaacagagau uggagggacg guucccacaa uuaacccaaa aacaaauauc ggucauagcu     1980

acauuaucau acaaaggcug gggaagguua agcagagaau uccuagaaaa uauaacuguu     2040

ccugcccccg ggacuggcga aguauggaau auaauuacag cauuauggga gacaaacgau     2100

aaucuaaugc aauuauuaua cggcgaauau ggcuuucaaa cagaaaucga augcuuuaau     2160

gcaaaagaaa aaaaaaaauc acucucguac gaaacaauag aagaaucgua uguuuccccu     2220

ucaguaaaaa gacaaauaug gcaaacccua caaguaguaa aagaacucaa aaaggucaug     2280

cgaggagacc cgaaaagagu cuucauagaa auggcuagag aaaaccagga aucuaaacga     2340

acagaaagua gaaaaaaaac ccuucuagac cucuauaaaa auuguaaaaa agaagagccu     2400

gaauggauug cagaacuuac agaacaucua gagaaauugg acgauucaca acuuagaaag     2460

gauaaauuau uuuuauacua uacgcagaag gggagaugua uguauucagg ggaaagaaua     2520

gagcuagaag accuauggga caacacuaga uacgauauag accauaucua uccgcaaucc     2580

aagacgaugg acgacucugu agacaauaga gugcuaguca aaaaagaaua uaaugcuaaa     2640

aaaacugacg uauacccaau agcagccgac auaagaggaa aaaugagucc cuuuuggaaa     2700

aaacuacuug acaauaaacu cauaucaaaa aaaaaauacg auagacuagu aagaacagaa     2760

gaacuuacac cagaagaguu agccggauuc auagacaggc aguuagugga gacccggcag     2820

agcacuaaag ucguagccga aauccuaaaa gaagcauucc cagacacaga aauagucuac     2880

gucaaagcag gaaacguaag caauuucaga cauauguuug auauaguuaa aguuagagag     2940

uugaaugacu uacaucacgc aaaagaugcc uaucuuaaua uaguaguagg aaacgcauau     3000

uacgucaaau ucaacaaaaa ugcagccugg uuuauaaaag aaaacccagg ucgcucauau     3060

aauuuaaaga gaauguucga uuaugauaua aggagaaaua acgagguagc augguuagcg     3120

gguucaacag guacaauaag ccaagugaaa aacaugguau cuaaaaauca cauauuagug     3180

acaagaaaga gcuacgaagu gaccggagga cuuuucgauc aacaacugau gaaaaaaggu     3240

aaaggccaag uacccguaaa aacgucagac gaaaggcuug gaaacauuga aaaauaugga     3300

ggauacaaua aagcuucagg ugcuuauuuu auguuaguua aaaguaaaaa uaaaaagggc     3360

gaagaacaaa gaacaaucga guauguaccc auauacuuaa agcauuauau agaagaguca     3420

aaagaaaaug cguuagacua uuuaaaaaac gaaaggaaau uaaaugaacc ugaaauacua     3480

cuugauaaaa uaaagaaaga cacacuauuu agaguuaacg gauucuacau guguuuaucc     3540

aaacgaacag gcaaacaauu agcauuuaaa ggagccaacg aacuacuauu accagaacca     3600

gaaaccuuau uauuaaaaaa aauuacuaaa uauauacagc gaagaaaaga gaauaaagau     3660

cuaaaacuca caaaacaaga caauauuacu gaaaaagaac uccuagaacu uuacgaugua     3720

uuuaccgaga aguuaaauca cucuguauac gcuguauguc ucggaacaca agguaaaacu     3780

uuaguccaaa aaaaaccagc auuuguagca cucccagucg aagaaaaaug uauuguacug     3840

ucugaaauac uacacuuauu ccagugccaa ucgggaagug caaaucuaaa acuaauagga     3900

ggaccagcau cugcaggaau acuauaucuu aacucuaaca uuucugacgc gggagaaauu     3960

uuaauaauca aucaaucacc aucaggaaua uucgaaaaaa gaauagauuu gcuacgacuc     4020

uga                                                                   4023


<210>  5306
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5306
augaaaaaag aauacacaau cggccuagac aucggaacca auagcguagg uuggucaguc       60

uuaacagacg auuaucgcuu aguaagcaaa aaaaugaaag uagcaggaaa uacugaaaaa      120

ucuucuacaa aaaaaaauuu uuggggagua agacuuuuug acgaaggcca aacagcugaa      180

gcuagaaggu caaaaagaac cgcuagaaga cgccuagccc gacgaagaca gagaauacuu      240

gaacuacaaa aaauauucgc ucccgaaauc uugaaaauag acgaacacuu uuuugcaaga      300

cuaaacgaaa gcuuucuugu ucccgacgaa aaaaaacaau ccagacaccc uguauuugcu      360

acaauaaaac aagaaaaauc uuaccaucaa accuacccca caauauauca ucuaagacaa      420

gcauuagcag auagcuccga aaaagcagau auccgauuag uauauuuagc cauggcacac      480

uuacuaaaau aucgcggaca cuuuuuaaua gaaggugaac uaaacacaga aaacucuucc      540

guuacagaaa cauucagaca auuuuuaagc acauacaacc aacaauuuuc cgaagccggu      600

gauaaacaaa cugagaaacu cgacgaagcg gucgauugcu cauuuguauu cacagaaaaa      660

augagcaaaa caaaaaaagc agagacauua uuaaaauauu ucccccacga aaaauccaau      720

ggauaucugu cucaguucau aaaacugaug guagggaauc aagguaacuu caaaaacgua      780

uucggucuag aagaagaagc caaacuacag uucagcaagg agaccuacga agaagaccuu      840

gaagaacuac uagagaaaau uggggaugac uacauagauu uauucgucca agcuaaaaau      900

guguacgacg cugucuuauu gucugaaauu cuauccgacu cuacaaaaaa cacaagagca      960

aagcucagug cuggcaugau cagaagguau gaugcacaua aagaagaucu aguccuacua     1020

aaacgauuug uaaaagaaaa ccuaccgaaa aaauauagag ccuucuucgg cgacaauucc     1080

guaaacggau acgccgguua cauagaaggu cacgcuacac aagaagcuuu cuauaaguuc     1140

guaaaaaaag aauuaacagg aauacgagga ucagaagugu uucucacaaa aauagaacaa     1200

gaaaacuuuc uacggaaaca aagaaccuuc gacaauggug uuauaccuca ucagauucac     1260

cucucagaau uaagagccau aaucgcuaac caaaaaaaac auuaucccuu uuuaaaagaa     1320

gaacaagaaa aauuagaauc uuuacuaacu uuuaaaauac cguauuacgu aggaccccua     1380

gcaaaaaaac aagaaaacuc accauucgcu ugguuaauaa ggaaaaguga agagaaaaua     1440

aaacccugga acuuaccaga aauaguagau auggaagggu cagcgguaag auuuaucgaa     1500

agaaugauaa acacagacau guauaugccc cauaauaaag uuuugccuaa gaacuccuua     1560

cuuuaccaga aauuuucaau uuacaacgaa cuaaccaaag uacgauacca ggacgaaagg     1620

ggacaaauga acuacuucuc aagcauugaa aaaaaagaaa ucuuucauga acuauuugag     1680

aaaaauagaa aaguaacuaa aaaagaucuu caagaauuuc uauaccuaaa auaugacauu     1740

aaacacgccg agcucagugg cauagaaaaa gccuucaacg caucauacac aaccuaccac     1800

gauuuucuaa caaugagcga aaacaagaga gaaaugaaac agugguuaga agauccggaa     1860

cuagcaucaa uguucgaaga aauaauaaaa acacuaaccg uuuuugagga cagagaaaug     1920

auaaaaacua gacucagcca ccacgaagcc acacuaggua aacauauaau uaaaaaauug     1980

acaaaaaaac auuauacggg uuggggaaga cucucgaaag aguuaauaca aggcauacgc     2040

gacaaacaau ccaacaaaac uauucuagau uaccucauaa augacgauga cuuuccccau     2100

caucgcaaca gaaacuucau gcaauuaaua aacgacgacu cacuaucauu uaaaaaagag     2160

auaaaaaaag cacaaaugau aaccgauacg gaaaaucuag aagaaaucgu uaaagaacua     2220

acaggcuccc cagcuauuaa aaaaggaauu uuacaaagcu uaaaaaucgu ugaugaaaua     2280

gucgguauaa ugggcuacga accggcuaau aucgucguag aaauggcucg agaaaaccaa     2340

acuacuggca gaggauuaaa aaguucccga ccuagacuca aagcguuaga agaaucucua     2400

aaagacuucg ggagucaauu acuaaaagaa uauccgaccg auaauucauc acuccaaaaa     2460

gauagauuau acuuauauua ucuacagaac ggaagagaca uguacacagg cgccccacua     2520

gauauacaua ggcucaguga cuacgauaua gaccacauaa uacccagauc auuuacaaca     2580

gauaacucaa uagacaacaa aguacuuguu ucauccaaag agaaucgucu aaaaaaagac     2640

gacguaccau cagaaaaggu uguaaaaaaa augagaagcu ucugguauga uuuauacuca     2700

ucaaaauuga ucucaaaaag aaaacucgac aacuuaacaa aaaucaaauu aaccgaggaa     2760

gacaaagcag gauuuauaaa aaggcaacuc guagaaacaa ggcagaucac uaaacaugua     2820

gccggcaucu uacaucacag guuuaauaag gccgaagaua ccaacgaucc aaucagaaaa     2880

guucgaauaa uuacccuuaa aucugcacua guuucacaau ucagaaacag guuuggaaua     2940

uacaaaguac gugaaauuaa cgaguaucau cacgcccaug augcauaccu caauggcgua     3000

gucgcacucg cauuacuaaa gaaauauccu caguuagccc cagaauucgu uuauggcgaa     3060

uaucuuaaau uuaaugcgca uaaagccaac aaagcaacag uuaaaaaaga auucuauuca     3120

aacauuauga aauucuucga aucagauacc ccagucugcg augaaaacgg agaaaucuuu     3180

ugggauaaau caaaaaguau ugcacaaguc aaaaaaguaa uaaaucauca ccauaugaau     3240

auagucaaaa agacagaaau ccagaaaggc gguuuuagca aagaaacagu cgaaccuaaa     3300

aaagauucau caaaauuauu accgagaaaa aacaacuggg auccggcuaa auacggaggc     3360

cucgguuccc caaacguagc auacacaguc gcauucacuu augaaaaagg aaaagcacgc     3420

aaaaggacaa acgcauuaga aggaauuaca auaauggaaa gagaagcauu cgaacaaagu     3480

cccguucuau ucuuaaaaaa uaaaggauau gaacaggcag aaauagaaau gaaauuacca     3540

aaauaugcac uuuuugaacu ggaaaacggc aggaaaagaa ugguagcauc caauaaagaa     3600

gcccaaaaag ccaacucauu ucuacuacca gaacaccuug ucacauuauu guaccaugcc     3660

aagcaauaug augaaauauc acauaaggaa ucuuucgacu augucaacga gcaucacaaa     3720

gaauuuucug aaguguucgc ccgcguacua gaauucgccg gcaaauauac auuagccgaa     3780

aaaaauauag aaaaauuaga aaaaauauac aaagaaaacc aaacggauga uuuagccaaa     3840

cuagccucau cauuuguaaa ucuaaugcaa uucaaugcaa ugggcgcacc ugcagacuuu     3900

aaguuuuuug auguaacaau accuagaaaa cguuacacau cccuaacaga gauuuggcag     3960

uccacuauaa uacaccaaag uauuacuggg uuauaugaaa caagaauaag aaugggcaaa     4020

uga                                                                   4023


<210>  5307
<211>  4023
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5307
auggaacaag aauacuaucu aggguuagac augggaacag gaucgguggg augggcagua       60

acugacagcg aauaucaugu guuaagaaaa caugguaaag cgcuaugggg aguaagauua      120

uucgaaagcg cauccacugc ugaagaaaga agaauguuua gaacaucacg cagaagacua      180

gaucguagaa acuggagaau ugaaauauua caagaaauau uugcagaaga aauaucuaag      240

aaagauccag guuucuuuuu acgaaugaaa gaaucuaaau auuaccccga agauaaacgg      300

gacauuaaug guaacugccc agaacuacca uacgcacugu uuguagauga ugacuuuaca      360

gauaaagacu accacaagaa auuuccuacc auauaucauu uaagaaaaau gcuaaugaac      420

acggaagaaa ccccggauau acgauuaguc uaucuagcaa uucaucauau gaugaaacac      480

cgaggacacu ucuuacucag uggcgauaua aacgaaauaa aagaguucgg aacaacauuu      540

agcaaauuac ucgaaaauau caaaaacgaa gaacuagauu ggaaccucga acuugguaaa      600

gaagaauacg ccguagugga aucgauucua aaagauaaca ugcuaaacag aagcacgaag      660

aaaaccagau uaauaaaagc acucaaagca aaaucaaucu gcgaaaaagc uguacucaac      720

uuacucgcag gcggaacagu gaaacugucc gacaucuucg ggcuugaaga auuaaaugaa      780

acagaacguc cgaaaauuuc auucgccgac aacggcuaug acgacuacau aggcgaagua      840

gaaaaugaac uaggagaaca auucuacaua auagaaaccg caaaagcagu auaugacugg      900

gcaguauuag ucgaaauacu aggaaaauac acauccauaa gugaagcaaa aguugccaca      960

uacgaaaaac acaaaagcga ccuccaguuu cucaagaaaa uuguaagaaa auaucuaacg     1020

aaagaagaau auaaagacau auucguauca acaagugaca aacucaaaaa cuauagcgca     1080

uauauuggua ugaccaaaau aaaugggaag aaaguggauc uccaaaguaa acgauguagc     1140

aaagaagagu ucuaugauuu uaucaagaaa aauguacuua agaaacuuga agggcaacca     1200

gaauaugaau auuuaaaaga ggaacuagaa agagaaacgu uccuaccuaa acaaguuaac     1260

agagacaaug gaguaauccc auaccaaaua caccucuacg agcuaaagaa aauacuggga     1320

aaucuuagag acaaaauaga uuuaauaaaa gaaaaugaag acaaauuggu acaauuauuc     1380

gaguuccgca uaccauacua uguaggacca uuaaacaaaa ucgacgacgg uaaagaggga     1440

aaauucacgu gggcagugag aaaaaguaau gagaaaauau acccuuggaa cuucgaaaau     1500

guaguggaua uugaggcuuc agcagaaaaa uucauaagaa gaaugacaaa uaaauguaca     1560

uacuuaaugg gugaagaugu ccuacccaaa gauaguuuac uauauucaaa auacaugguc     1620

uuaaacgaau ugaacaacgu aaaauuagau ggagaaaaac uaaguguaga auuaaaacaa     1680

aggcuauaua cugacguauu cugcaaauau cgaaaaguga cggucaagaa aaucaaaaac     1740

uaccuaaaau gugaggggau aauaucagga aauguagaga uaacuggaau agauggcgau     1800

uuuaaagcua gucucacggc auaucacgau uucaaagaaa uauuaacugg aaccgaacua     1860

gcgaagaaag auaaagaaaa uauaauaacc aauauaguac uauucgguga ugauaagaaa     1920

cuacucaaga aaagauuaaa cagacucuac ccucaaauua ccccaaauca acuaaagaaa     1980

aucugcgcac uaucauauac uggauggggu agauucagua agaaauuucu ugaagaaaua     2040

acagccccag auccagaaac cggcgagguu uggaauauaa uuacagcucu augggagagc     2100

aacaacaacu uaaugcaguu acuaaguaac gaauacaggu uuauggagga aguagaaaca     2160

uauaacaugg gaaaacaaac aaaaacuuua aguuaugaaa cuguugaaaa uauguauguc     2220

aguccgucag ugaaaaggca gauauggcaa accuuaaaaa uaguaaaaga auuggaaaaa     2280

gugaugaaag aaaguccaaa aagggucuuu aucgaaaugg cacgagaaaa acaagaaagc     2340

aaacggacag aaucgaggaa gaaacagcuc auugacuuau auaaagcaug uaaaaaugaa     2400

gaaaaagacu ggguaaaaga auuaggagac caagaagaac aaaaacuucg auccgacaaa     2460

uuauaucugu auuacacaca gaaaggccga ugcauguaca gcggcgaagu aauagaacua     2520

aaagaccuau gggauaauac aaaauaugac auagaccaua uauacccaca aucaaaaacc     2580

auggaugacu ccuuaaauaa uagaguauua guuaaaaaga aauauaacgc cauaaaauca     2640

gauaaauauc cucuaaauga aaacauacga cacgagcgca aaggauuuug gaaaucccuc     2700

cuagacggug gauuuauaag caaagaaaaa uacgaaaggc uaauacguaa uacugaacuc     2760

ucaccagaag aguuagcagg auucauagaa aggcaaauag uugagacccg acaaucaaca     2820

aaagcugucg cagaaaucuu aaaacaggua uucccagaau cagaaauugu auauguaaaa     2880

gcaggaacgg uaagucgauu ucgaaaagau uucgaacucc uaaaaguaag agaaguuaac     2940

gaccuacacc acgccaaaga ugcauauuua aauauaguag uuggaaauag uuauuacguu     3000

aaauuuacua aaaacgccag uugguucaua aaagaaaauc ccggaagaac auacaaucua     3060

aagaaaaugu uuacaucagg uuggaauaua gaaaggaacg gagaaguagc cugggaagua     3120

ggcaagaaag ggacgauagu cacgguaaaa cagauaauga auaaaaacaa cauccucgua     3180

acaaggcagg uacacgaagc aaaaggagga cuauuugacc aacaaauaau gaagaaagga     3240

aaaggucaaa uagccauuaa agagacugac gaaagacuag caaguauaga gaaauaugga     3300

ggauacaaua aagcagcagg cgccuacuuc auguuaguug aaucuaaaga uaagaaagga     3360

aaaacaauaa gaacaauaga guucauaccu cucuacuuaa agaacaaaau agaaucugau     3420

gaaucaauag cauuaaauuu ccucgaaaaa ggaagaggau uaaaagaacc aaaaauccuu     3480

uuaaagaaaa uaaaaauaga uacauuauuu gacguagaug gcuuuaaaau guggcuuuca     3540

ggaagaacag gagauagacu ccuauucaaa ugcgcgaacc aauuaauacu agaugagaaa     3600

auaauaguaa cuaugaaaaa gauagugaaa uucauacaaa gacgacaaga aaaccgagaa     3660

cucaaauuau cagauaaaga cggaauugac aacgaaguuc ugauggaaau uuauaauacg     3720

uucguagaua aacuugaaaa caccguuuau cgaauaagac uuucagaaca agcaaaaaca     3780

cuuauagacc gacaaaaaga auuugaaaga cuuucauuag aagacaaaag uaguacacua     3840

uucgaaauuu uacauauauu ucaaugccaa ucaagcgcag cgaacuuaaa aaugauagga     3900

gggccaggaa aggccggaau ccuaguaaug aauaauaaua ucucaaaaug caacaaaauc     3960

ucaauuauaa accaaagucc caccggaaua uuugaaaaug aaauugauuu acuaaaaauc     4020

uga                                                                   4023


<210>  5308
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5308
augagguucc aaaacaagaa aacgggagau uacuuugucg gucucgauau agggaccaau       60

aguguugguu gggcuguaac uaaugaaaaa uacgaaauau uaagagcuaa aaaucauaga      120

auguggggcu caagauuguu cgaagaaggc aaaacagccg aagaacgaag aguacacaga      180

gcaggucgcc gucgcuuaaa ccgacgaaac gauagauuaa ugaucuugga agaccuauuc      240

gcggaagaaa uaaguaaagu agacaaagcg uucuaccaaa gacuacauga aucacaauac      300

cgacaagaac auaaaacggu uagcggaccg uacuuccuau uuaacgacaa agaauuuacu      360

gacgaagacu accauauuga cuacccaacu auauaccauu uacgagucgc uuuaaugaaa      420

gccccagcaa aagauguacg cuuauuauuc uuagcaauuc aucauauaau aaaauauaga      480

ggaaacuucu uacacgaagg cgaaacauuc aaaacaacag guggacucac ugauauauua      540

caacguguuc uagcaugcaa ugaguucagu gguuuaaaac aacacaucga ugacaccgcu      600

uuaguuaaau ugcuaaccaa uaaagguauu acaagaagcg acauagcaaa acaaaucuca      660

gaacucgcaa cagauaaacu cuacagaaag caagccaaag cauuugccuc gcuaguacuu      720

ggccuaaaag caaacuugga ugauuuauuu aacgaaccag aauacaaaga auuagacuca      780

gaaauuagga ccguggaauu uucaggcaaa gaauauuggg aagaacgaga caaguaugaa      840

ccauacuugg gagaaucucu cuucgcucug gacggaugua aaacauuaua cgauacauua      900

cuauuaucaa auauaaaacc cgauggaaaa uauuuaucgg aagcaaaagu agaaacauuu      960

gaaaaacacc acgaacaucu ggaaucgcuu aaagacuuau uaaaaucaaa caaagcacua     1020

uacaaucgag uauuaagaga ugacgaaaag aauuuaaaua auuauauaac auauauuggu     1080

aaaagugaau uuaacaaaag auguagucga gacgaauuuu auaaauuccu uaaacaaaca     1140

auaaaagaua uaagcgacaa ugaaaauaaa gcugaaauug uacaauuaau ggaacucgau     1200

ucauuccuuc cacuauuaag gguacgagaa aaugguguca uuccuuacca aauucacgaa     1260

gaagaauuag aacaaauauu aaauguuucc ucagaaaaau acccauuuuu gaacaaagcg     1320

gacggcucag gauucaccau cgcgcagaaa auucuaaaac uccuuaccuu cagaauacca     1380

uacuucguag guccccucaa cccagcacau aaagauaggg acaacggguu cgcaugggua     1440

guaagaaaag caccagguaa aguuuacccu uggaauuucg aagacaaagu ugauauaucc     1500

gcuucagcaa ccgcuuuuau agaaaacuua accaacaaau gcacauacuu gauaggagaa     1560

gacguacuuc ccaaauacag cuuagcauau aguaaauuua gccuacuaaa cgaauuaaau     1620

aaucuaagau acgacggcaa accauuauca uaugaaacaa aacagagcuu uauaaaggau     1680

uguuucgaaa aagaacaccg uaagaugacc gagaaaagga uacgagaauu uuuaaaacau     1740

uuaggaggaa cagacgguaa aggagcauua acaggacucg acguagauau caaaucagau     1800

cuuaaaucuu aucgagauau gguacggaua uugggcgacg gauucgacga aaccauggca     1860

gaacgaauaa uacguuaugu uacacuauuu ggugaagcua agaaaguauu auuaaguaca     1920

cuagaaauag aauuuggaga cgacuugucu gauacacaaa agaaacaaau auuaaaacua     1980

caauauagag auuggggaag acuaucagcu acccuacuaa aagguauuaa aggggucgcc     2040

ggagauaauc cagaaugcuc cauauuagaa ugcauggaac aacaaccguu aaauuuaaug     2100

gaaguguuau caucacauaa cacuuauuug gcagcaauug aggaguucaa uaaagaaaga     2160

acugaaccca cugacauugu agacuauaaa uuaguagaug accuuuacau uucgccagcu     2220

guaaaacgcu cgguuuggca aacgcucaga auauuagacg aaauaacaaa acucagaggc     2280

uccgucccua agaaaguauu uguugaagua gcuagaacaa aucgugcaga gaaaaagaaa     2340

accgauagua gaaaagaaag auuauuacag cuauacaaaa caauaaaaga cgaaggaagg     2400

aacuggacag aagaaauuaa acaaagagau gcuggggauu ucaacagcaa gaaacuuuuc     2460

uuguacuaca cccaacaagg ccgauguaug uacucaggug agaaaauaac caguauuggu     2520

gacuuauuua cgagcaaaua ugauaucgau cauauauacc caagaucggu cacuaaagau     2580

aacucauggg acaacuuagu cuuagucaaa gcagaacuaa accgcgaaaa gaaagacauu     2640

uacccaaucg aaaaagacac acaggacaga ugcauaagcc uauggaaaca gcuucucgaa     2700

caaggacuca uaucagaaag aaaauacgaa agacugguca gaacaacccc acuaacagca     2760

gacgaacuag cagacuuugu gaauaggcaa cucguagaaa caaaccaaag uguaaaagca     2820

guuaccaauu uauuaaaaag acuguaccca gacaccacaa uaugcuaugu aaaagccgaa     2880

aauguauccg acuucagaca ggaaagagaa uuugugaaac uuagaucacu aaaugcacac     2940

caccaugcaa aagaugccua ccucaauaua gucguuggaa acguauacca ugaaaaauuu     3000

acuaacaacc cacgcaacuu uauuaaaaau gcacggucag acaagaaaag accuuacagc     3060

cuaaaucaca uguuugauua ucuccuacaa guagguaaua aagaaauaug gaaucccaaa     3120

acugauaaua aucucgucaa gaaaaugaug cgaaauaaug auguacaagu aacuagaaug     3180

cucguagagc aaacuggcgc acuguacgau gcaacaauau acaaggcuuc agugacaaaa     3240

agcgaaucau auguaccacu aaaaucaucc gaugccauuu uaucagaugu aaagaaauau     3300

ggaggcuuca caucaaucaa aauugcauau uauucgauau ucagguucua caagaaaaac     3360

ucagaagaau uugagacaca uauaauaagc auaccuguau uaaaagcagg cacacucaau     3420

agcaaagaag auuauguagc uuacggagaa gagauaauga auaaacaaaa auucgacaga     3480

guagagcuag uuuauaagaa auuauguaug aacucacucg uacgaauaaa cgguguuaug     3540

uauuaucuag gagggaaaac caauaacuca cuuuauauag auucagcaau cagccuagua     3600

cugccagaca auauagcaaa gaaaauaaaa gccguagaaa aauauauagc acgugucgca     3660

gaaaacggca auuauaaacc acagccagac caagucucac cugaguucaa uaugauuaua     3720

uauaaggcau uaacagagaa aaugaaacaa cccgcauucg uaggauugaa aacaaaucgg     3780

gaaaaugaac uggaaaaaga aaccacaaua acagccuuua uagcaaugac acuaauagaa     3840

caauguaaaa ugcuaaugga aauacuaaau cuacucacac aacagaaaac aacauaugcu     3900

cuaaaaccaa ucgguguguc agcaucaaga ucuacacuag gaaugaauau aacagguuua     3960

agcgaauuua caauaauaac ucaaaguguc acagguuuac aagaaaaaga aauaagaauu     4020

aucuga                                                                4026


<210>  5309
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5309
auguggggau cccgacuauu cgaagaagca uccacagccc aagaccgcag agaacacaga       60

agcaacagac gacgucuaga aagacgaaaa uaucgucugg accuguuaga agaacuauuu      120

guagaagaaa uaaacaaaau agauccagaa uuuuucauua gacuaaggga aaguaaauuu      180

cauuucgaag auagaucacu uaaaaauaca uuuauacuuu uuaacgacaa aauggaagga      240

gguuacaacg acaaaguauu cuuuucaaaa uacccuacaa uauaccacuu aagaagugca      300

cuaaucaaug gaaacaaaga agacgucaga gcaguguucc uagccauuca caauauacuc      360

aaaaaccgag gacauuucuu acuagcugga aacgauauaa acauuucacu aucccugaau      420

gauacguuaa cagauuuauu cgaauaucua aaaauacacc uaggaauaga aguagaguuc      480

gaaaauaaau uaaaagacau cgagaaaaua gcccuagaca aaaaucuauc aaaaagcgau      540

aaacagaaga aaauaguaca acuacuagaa acagacuaua agaaacaauu agcugaugua      600

uucaaacuau cauuaggucu gacagcaaac auggaaaaca uauuuggcaa agaaaacgaa      660

caaggcuuag aauuuauaaa ugccgaaaau aaaaucaaga aaauacaauu cgacaguuca      720

uacgaagaaa auauugcaga auuugaucaa uucuacggag aauauuucga aauaauagac      780

auuuguaaga aaauauauga ugcaauaucu cucucucaaa uccuaguaga cgguaaaggc      840

cucucagaaa gcaaaguguu aucguacgaa aaacacaaag aggacuuaaa acuacucaag      900

aaagugcuaa agaacauaga caaagcguau gauaacaaag gagaagacag auuaguauac      960

aaaguucuua aagaagacaa agauaaagga acgaacuacg uaaauuacag uaaauuauca     1020

aauaacggaa cagcaacuac aaaagaagag uucuacaaau uccugucuaa aaaccuggau     1080

cuuaagaaau uuggaaaacu aaacaaagaa gauauagaaa ccuuagauua cauaaacuca     1140

gaaauacuau uaggcagauu ccuaccucua caaaaagaga aaaucaacgg aguaauacca     1200

uaucaauuac aaaugaaaga acuuacccaa auucuaaaca augcaagcaa auaccuacca     1260

uucuuaaaca aaauagaaga uggaaugacu guuaaagaca aaauaauauc uuuaauuaaa     1320

uuccguauac cauacuaugu cgguccgcuc aacgaauaua acagaaucga aaacggaaac     1380

gguuuugcau ggauagagaa aaagaaaaau gagaaaaucu uucccuggaa cuuuaacgac     1440

gucgucgaua uagaaaaauc cgcagaaaaa uucauuaaca acuugacaaa uaaauguacc     1500

uacauaaaag aagaaaccgu ucuacccaaa aacaguauaa uauauucaaa auuuaugguu     1560

cuuaaugaac ucaacaauuu acaauacgga aacaagaaac uagacauaga acuaaaaaug     1620

aaaauguaua cugaccuuuu ucuugaaaag aaagacaaua gaaaaguaac agagaaaaga     1680

auccuugaau auuucagaau aaacggcaua aacgauggaa aagaaaaacu aaccggacua     1740

aacaacgaaa uacaugcaga caugaaaucc uaccgagaca ugugugaaau aaugggagaa     1800

aacuucaaca uaaaugucgc agaaaauaua aucaacugga uaaccguauu ucacggugag     1860

aagaaaauac uuauuaauaa aaucaaaaau guauacggca aaauguucaa cgacgaacaa     1920

aucucaaaac uauccaaacu aaacuauagu gauuggggac gacucagcaa gaaacuacuc     1980

acagacauaa aauguuauau agaugcagac gguauaaauu ucccuguauc aauccuggaa     2040

gcaauggaag ucuccaacaa aaaccuaaug gaguuacucu caaacaaaua ugaguucaua     2100

ucaugcaucg acgacauaaa caacaaauac uucggagaua gggacauauc ugcaagcgaa     2160

cucaucgacg aacuauaugu aucaccagca guuaaacgag guauauggca aucaguacgc     2220

auuuuagagg aaauaguaag caucauagga cacaacccca agaaaauauu cauagagaug     2280

acccgacaca augaagaaaa aguucagaaa ucaucaaggc gucacaaucu ugaggaacuc     2340

uauaaagcca uccgcaaaga cccagaauac guagaacuga ucaaagaauu ugauagcacu     2400

acugacacag auucauucaa aauaaagaaa uuauaucuau auuucaugca acuugggaaa     2460

ugugcauauu caaaagagaa aaucgacuuc gacaaauuau uuacugauuc cuacgacauu     2520

gaccauaucu acccaagauc uaaaacaaaa gacgacucua uacauaacaa ucuaguuuua     2580

gucaaagcaa aauauaauag agagaaaacu gauguauacc caaucgauau aaauauccag     2640

aaaaauauga acucauuuug gaauuuccuu uacaccaaga aauucaucac agaagaaaaa     2700

uauaaacgcu ugauacguac aagcgaccua acagaugaag aauuggcaaa auuuauaagu     2760

agacagcuag uagaaacgag ccaaaguacc aaagcaacag ccgaaguacu gaaacaaaua     2820

uauggagaca gugacauuug cuacguuaaa gcagaaaacg uuucagauuu ccgauaugga     2880

agcucccguc aaaaagauaa aaaugauaua caaaaacuau uuguuaaaug ccgugaaguu     2940

aaccauuucc aucaugcgaa agacgcguau uuaaacauug uugucggaaa cguguacgau     3000

guaaaauuua ccaaaaaccc cgucaacuuc aucaagaaaa acaaaauggg caacaaaucg     3060

aauuauucuc ugaaccacau guuuuguuuc gacguugaac gaaacggaua caaagcaugg     3120

gauucuuccg aaaccauaaa aacaguaggc agaaugauga aaucaaacga cgugagaauu     3180

acgaaacaug uccacccaac aagaggagaa cuguacgaug caaccauaua caaagcaaaa     3240

auugcaaaaa cuggcucaua caugccuuug aaaucaagca aaaacacccc acugagcgau     3300

guaacaaaau auggaggaua cacaaaaaua aaaaucgccu auuacuccgu auauaaguac     3360

aaguucagug acaaaaaccu caucaaaacc gaaauaagac ugauaccaau cccaauauac     3420

cuaagcaaaa acgacgauga cauaaaagaa uacgucaaaa auuaccuaaa cgacaggguu     3480

aauucaaaau uaauagacuu cacaaaaauc uacgauaaau uauacguuaa uucacuaaua     3540

aaaauaaaug gauauuuaua uuaccuuggc gguaaaacaa augauagagu cuggauagau     3600

aacgccguau caauagucuu ggaugacaag auagaguacc uaauaaaaag gauaucaaau     3660

gauauaaaau auggguacga aauaaacgug agugaugacg aacuaaugga caucuaugaa     3720

ucacucgcag aaaaagcgaa caaaaagaua uuuuacaaca gacuuucaga cuucgaaaag     3780

auauucucaa auauggaugu caaaaagaaa uucgucaagc uagagaaaaa cgacaagugc     3840

aggguauuac uccaauuacu uaacguauua acagacagcc aaacaacaua caaacuggag     3900

augguaggau ugaaacauuc aaggaguaac auaggaauga aaaucucaaa cuuagaagag     3960

uucaaaguaa uaaauuccag uauaacagga cucuauucaa augaaauaga uauuuuaaau     4020

auauga                                                                4026


<210>  5310
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5310
auggaaaaag auuacuacuu aggacuggau augggauccg gaucagcggg augggcagug       60

acugacgauc aauaucacgu acuaagagcu cacggaaaag caaugugggg uacgcgcuug      120

uucgaaagcg cuaauaccgc agaagaacgu cgaauaaaua gaacagcacg aagaagacua      180

aaacggagaa accaaagaau agaaugguua caagaaauau uugcggaaga aauuaacaca      240

aaagacucug gguuuuuccg gcgaaugaaa gaaucgagau acugggcaga agauaaacga      300

gacgaaaaug gaaguugccc agaaaugcca uacagccuau ucauagauga agacuuuaca      360

gauaaagaau accaugaaaa auucccuaca auauaucauu uaagaaaaua uuugaugaca      420

acagaagaaa caccaggaau aagauuaguc uacuuagccu uucaccacau gaugaaacau      480

agaggacacu uuuuacuuaa cggaaauaua gaagaaauac gaaaauuucg aaaaaccuuc      540

gaucaauucu uagaaguagu cagaaacgaa gaaaugggau ucgcaggcga aauagaagaa      600

gaaacauaua agaaaauaga aaugacuuug aaagacguaa auuuaacucg gucagccaag      660

aaaacaacau uaauaaagag cuuaaaaguu acuagaguug aagaaaaaga acuacuagca      720

uuaauaagcg gauguacggu caaacuauca aauauauuua augauaaauc cuuagaugag      780

acggaaagac caaaaaucag uuuugcagac gcuggcuucg acgacuauga agcagagaua      840

caaaguaacc uuaacgaaaa auacuacauc auagaaagcg caaaagcugu auacgauugg      900

gcugcauuag cagauauauu gggggaauac aguuguauaa gcgaagcgaa aguugcacua      960

uacgaaaaac aucauaaaga ccucaaaaga uuaaaagacc uaguaaggaa uaacaucucu     1020

ucagaaguau auaaagaaau uuucgucgac gucaguggaa aauacaaaaa uuauugcgcc     1080

uacaucggaa cuacaaacau uaauggcaag aagaaagauu ucgaaggcaa gaaaugcaca     1140

aaagaggauu ucuacgccua ucucaagaaa ucaguuauag aaugcauaaa cgauagcgag     1200

aaaacagacu ggcucaaacu ggaauuagaa aaaggaaacu uccucccaaa acaauugacc     1260

ggcgaaaaua guguaauacc cuaccaaguu caccuuuaug aacucaacgc aauaauaacu     1320

aaccuccaag aaagaauacc auuacuuaaa agagacggag agaaaauucg acaaauauuc     1380

acauuucgug uaccauauua ugucggccca uuaaacggaa uaagcauaga ugggaagaaa     1440

acaaacuggg uaaaacgaaa agaaggugug auuuacccuu ggaacuuuuc agacauagug     1500

gacgaagaaa gcucagcuga aaguuucaua aggcgaauga caaacaaaug uacauaccuu     1560

auacacgaag acgugcuacc uaaauauucu uuacuauaua gcagauucga aguucucaac     1620

gaauuaaaua augucagguu aaacggggaa cuauuaagug uagagcuaaa acagaaaaua     1680

uacgaggaug uauuucaacg uuauagaaaa gucacgcaga aacgacuuaa acgauaccuc     1740

cuaacagaag gaauaauaga cgaaaacaca guuauaacag gccuagacgg cgauuucaaa     1800

gguaguuuaa cugcauauca cgauuucaaa gaaaaacuca ccggauuaaa ucuaacugaa     1860

cgugaaaaag aagaaauaau uuuaaauauc acgcuauucg gggaugcgag gagcuuauua     1920

agaaaacgga uaggaaaaau augcagucaa cuaucagaca aacaaaaaga aaaucuuagc     1980

aaacuuagcu aucaaggcug ggguagauug uccaagaaau uuuuaaaugg uauagcgauc     2040

agcguccaag gagaagaaga auggacaauu auuaaagcac ucugggaaac ucaagauaac     2100

cugagucaac ucaugggugc aaaauauaca uuuagaaacg caguagaaaa agagaacgga     2160

gaacucgaaa acagagaaau uaguuaugaa acaguaaaag acuuaucaau aagcccaucu     2220

guaaaaaggc aagucuggca aucacuucua auagugaaag aaauauguaa aauccagaag     2280

aaaccuccac gaaaaguauu cguagaagua gcaaaagaga aaacuaacag ugguaggacg     2340

gaaaguagga agaaaagacu ucaagacuuu uauaaauauu guagcaaaua cgaaaaagaa     2400

cuauacgaug aaagcaucca aaauaaauua gaaaaaugcu cagaaucaga ucuaagaucc     2460

gacaaacuau ucuuauacua caugcaaaug ggacgaugua uguauacugg gcaacccauc     2520

gacuucgaug aacucuggaa caaccaaaaa uaugauaucg aucauaucua cccacaaagu     2580

aaaaccaugg augauucaau agacaacaga gucuuaguaa aaaggaucuc aaaccaagag     2640

aaaacagaug uguaucccau aagagcagau auccgagaaa agagaagagg uuuuuggaaa     2700

gaacucaaaa acguuggccu aauuagugaa gaaaaguaua aacgacuaac aagagcuacu     2760

gaauuugaac cagcagaacu agcaggcuuc aucucaagac aauugguaga aaaccgacag     2820

accacaaagg uagucgcaag cauuuugaag caagccaugc cggacaccga aaucguauau     2880

gugaaagcac gaaccguauc ugaauuucgg cacaaauuca acuucauaaa aguuagagaa     2940

augaacgacu uccaccaugc aaaggaugca uaucucaacg uuguaguggg aaacacauac     3000

cuaguaaaau uuacaaaauc acccguaaau uuuauugaga accacucaaa ccguagcuac     3060

aaucuaaaga aaauguucac agauggaaga guagaacgga auggcgaaau agcauggaua     3120

uccggagaaa augguaccau aggcacagua agaaacauaa ugaagaaaaa caauauucuc     3180

gucacgagga gagcacacga ggcgacaggc ggguuuuucg accaacaacu aaugaagaaa     3240

ggaaaaggac aagugccugu aaaagguagu gaugaaagac ugcuagacau ugaaaaguau     3300

ggaggauaua acaaagaaac aggagcauac uucgucuuag ucgagagcga cgagaagaaa     3360

ggcaaaaaga aaagaacaau agaauaugua ccgauaagac ucaaaaagca acuagaaaac     3420

aauagaaaag cacuagaaaa cuacuuaugu gaacaaagag gacuaacaaa cccagucgua     3480

uuaaucgaca agauaaaaau ggauucuuua uuccguguag auggcuucuu cauguggcua     3540

uccggaagaa ccggaaacca auuacuauuc aaaugugcga aucagcuagu cuugucuacc     3600

gaagacgagg cgacauuaaa gaaaauagua aaauuuguaa accgacgaaa agaaaauaaa     3660

gagauaaaaa uauacgaaag ugauaaacua aacgaagaaa uguuaaacag acuauacaac     3720

acauuccuuu acaaacuuca aaauucaaua uauggaaacc gguuaggcga gcaagcuaaa     3780

acacuaucua guaagaaaga aaauuuugac aaacugcaga uugaagaaaa augccuaguc     3840

cuauacgaga uuuuacauau guuucaaugu caaagugcaa ccuccgaccu acaacuaaua     3900

gagggaccag gcaaagcagg aacacuaagg uuaaauucga auauaacuaa auacaaaaac     3960

aucaauauca ucaaccaauc accaacggga auauacgagc aaguaauaga ccuacagaaa     4020

auauga                                                                4026


<210>  5311
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5311
augggcaaag guuauuacgu aggccuagac auggguacug gaaguguagg augggcagua       60

accgaugaau cauaccaaau acugcgacgc cacggaaaag ccaugugggg aguuagauug      120

uuugaaagcg caaaaaccgc ugaagaaaga aggauguuca gaacaggaag acgaagguua      180

gacagaagag guuggcggau agaaauucuu caagaaauau uugcggaaga aauaucccga      240

guagauccag gcuucuuucu ucgaaugaaa gaaagcaaau acuacccaga agauaaaaga      300

gauauacagg ggaacugucc cgagcucccu uacacuuuau uugucgacaa aacuuucacg      360

gacaaggacu uccauaaaaa auaccccaca aucuaccauu uaagaaaaau guugauggaa      420

acagaagaca caccagauau gagauuaguc uaccuagcgc uccaccauau gaugaaacau      480

agaggacacu uuuuauugag cggagauauc agccaaguaa cagaauuuaa aaacaccuuc      540

aaccaguuca uagaaaauau aagaaaugaa gaaauggacu uugaaauuga auuagacgaa      600

agcgccgugc agaugauaga ggaaacauua aaagauaaaa accucacccg aucugccaaa      660

aagucuaaau uaguuaaagg ucucaaugcu aaaaaugucc gagauaaagc auucuuaacg      720

cuuuuaucag gaggaaccgu gaaguuauca gaucuauucg gaauggaaga acuaaacgaa      780

ggugaaaggc ccaaaauaag uuucgcagac aaugguuacg aagaauaugc cgcuguggua      840

gaaauggagu uaggagaauu auauuauaua guagaaucag cuaaagccgu uuacgacugg      900

gccaucuugu cagauauccu aggaggcuca acaucaguaa gugacgccaa aguccgagca      960

uaugaaaaac acaaagcaga uuugaaauac uuaaaagcag uaguuaaaga auauuuuccu     1020

aaggaagugu auaaugcagu auuuguagaa ucauccgaua aacugaauaa uuauccggcg     1080

uauaucggaa ugacaaaaaa aaauggaaaa aaagucagcc uagaaggaaa aagaugcucu     1140

agggaagagu ucauggauuu ucugaaaaaa aacauugugg uuaaauuacc agaugaggag     1200

gcaaaaaugu aucugcaaag ugagcucgaa aaagauaguu uccuacccaa acaaguaaac     1260

aaagauaacg gaguaauacc auaccaagua cauaaauaug aauuaaaaaa aauacuagau     1320

aacuuagggg auaaaauucc cuuccuaaaa gagaaugcag aaaaaauaga gaaauuguuu     1380

aguuuuagaa uaccguauua cgugggacca cuaagaacag guaauggaga aaauuccaaa     1440

uuugcauggg cagaacgaaa aucaacagaa aagauauauc cguggaacuu ugaaaacguu     1500

auugacguug aacaaucagc cgaaaacuuc auaagaagaa ugacaaacaa auguaccuac     1560

uuaauaggug aagauguucu accgaaagau agucuacucu acagcaaguu uaugguauua     1620

aacgaauuaa auaauuuaag acuugacgga caaaaaauau cagucgagcu aaaacaaaaa     1680

auauauagag auguauuuug uagaucacga aaaguaacac aaaaaaaguu aaaaucguac     1740

uuaauaaggg aagggaucgc uggaaaaaau guagaacuaa cugggauaga cggagacuuu     1800

aaaggaucgc uaacggcaua ucaugauuuu aaagaaaaac uaauagacgu ugaacugacc     1860

cagaaagaaa aagaagagau aauauuaaac auuguguugu uuggagacga caaaaaacuc     1920

cugaaacaaa ggcuuaaaaa aaaauuccca caauuaacgg aaaagcaaau caauacagua     1980

acagccuuau cauacaaagg augggggaga cuuucaaaga aacuucugga agaagucacu     2040

guaccugcuc ccgagacugg agaaguaugg aacauaauaa cggcauuaug ggaaacuaau     2100

gacaauuuua ugcaacuacu uagcaaagaa uaccaauuua gugauagaau agaagaguuu     2160

aacgaagaaa aagcagaucc ugaaguauca uaucagacga ucgaagaucu uuauguaagu     2220

ccagcaguua agcgacaaau uuggcaaacg uuacaaauaa uucaagaaau agaaaaaguu     2280

augggugaga agccaaagcg uguuuuuguu gaaauggccc gcgaaaaaca ggaaaacaaa     2340

agaacagaaa guagaaaaaa gacgcuauca gaucuauaua caaaauguaa gaaggaagaa     2400

ccagaaugga uagaagccuu auguacaucu cuagaaaaac augaagaaca ucaauugaga     2460

agcgacaaac uauauuuaua uuauacacaa aaggggagau gcauguacuc aggggaaccg     2520

auagaucuag aagacuuaug ggacaauaca aaauaugaua uugaucauau auacccccag     2580

ucgaaaacca uggaugacuc ucuaaaaaac agaguucuag uaaaaaaaga auauaacgca     2640

aagaaaucgg acacauaccc gauagcggcc gauauacaga aaaaaaugau gcccuucugg     2700

aaguccuuac uaacuggagg uuucauuccu aaggaaaaau acgaucgauu ggucaggaau     2760

aauccacuag augcaaacga acuagcagga uucauagagc gacaaaucgu ugaaacgaga     2820

caaagcacaa aagcuguagc agaaauauua aaaaaaauac uacccgauac agaaauagua     2880

uaugucaaag cgaaaacagu aucaaaauuu agacaagacu ucgauuucau aaaagugcga     2940

gacaugaacg aucugcacca ugcaaaagau gccuaccuaa auaucgucgu aggaaaugua     3000

uacuuuguaa aauucacuaa gaaugcagcu ugguucguga aagaaaaucc aggcaggaca     3060

uacaaccucg aaaaaauguu uauaugggac guagcuagag acggagaaau agcauggaag     3120

gcuggaaaaa guggaaguau uguacaagua agagcaauga uggaaaaaaa ucacauccua     3180

guuacacguc gaaguuacga aguaaaggga gggcuauuug aucaacaaau auuaaaaaaa     3240

ggaaagggac aaguaccagu gaaggaauca gaugagcgac ucagagacau aggaaaauac     3300

ggaggcuaca acaaagcugc cggcguauac uuuaugcuag uaaguaguaa agauaaaaag     3360

gggaaagaac aacgaaccau cgaauuugua ccaauauacu ugaaagauag agucgaaaaa     3420

gauauggaaa gugcaagaaa uuauuugaug gaagaaaggg gauuacgaga accuaagauu     3480

cuuauuaaga agauaaaaau agauacauua uucaaaguug acggauucua uauguggcua     3540

aguggaagaa cauccaauca auuaauuuuu aaaggcgcaa aucaacuuau uuuaaguaaa     3600

gaagauauga aaacauugaa aaaaguugua aaguacauac aaagacaaaa agaagacaaa     3660

aauguaaaaa uaaguaugcg agacggaaua acugaagaag aauuauuaaa acuuuacgaa     3720

acguuucuag auaaaauaag acauagcaua uacggagcaa gacuaucuuc acaggaaaaa     3780

acacugguaa acggaaaaga aaaguuuaua gacuuaucag uagaagcaaa augucaagua     3840

uuauacgaaa uacuccauuu auuccagugc caaagcggag ccgcaaaucu aaagaugauu     3900

ggaggacccg caucagcagg aauuuuagua augaauaaua auauuaguaa auguaaaaac     3960

aucaguauag uaaaucaaag cccaacagga auauaugaga aagagauaga ucuacuaaga     4020

guauga                                                                4026


<210>  5312
<211>  4026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5312
augacacaaa aagugauacc uuauaauauu ggacuugaua uaggcacaaa uuccguuggc       60

ugggccguaa cggacaacga aaacaaauug uugcauauaa aagggcacaa cgggauaggc      120

guacgucucu ucaaagaagg ccagagugcg gccgaaagau gcggcuucag aacaacaaga      180

agacgucuca guagaagaaa auggcgauua agguuacuua augaaauauu ugccgacaaa      240

auagcaaaag uagaccccag cuucuuugca agacuuaaac aaagcaaugu uucaccaaaa      300

gaucccaaca aaacaauguu uggaaauauu cuauuugaua augggaaccu uaacgacaaa      360

ucuuuucacg augaauauaa aacgauauau cauuuaagac aaucaauaau ugaccaccca      420

gaaaagaaau uugacauaag acucauuuau cuagcaaugc aucauauaau aaaauacagg      480

ggacauuuuc ucaaccaagc caauguacaa gauuucaaag gcggaaaaau agaccuagaa      540

gaaucauuca aagaucuuaa uagcaucuuu gaaaaccagg gccgagaucu aaggcuaguc      600

acagaagaug caaauaaaua cguaaaagaa uuaucagaca acucuaaaac ucgaaacgau      660

cgacaaaaag agcuaucaaa agucuuguac auuccuauag acaaaaacuc cgacaaaaau      720

aauaagaaag ucacuacaga aauaauaaaa gcaauacuug gaaugaaagc aaaauucgau      780

guaaucuucg gcauugaugc cgagaacgcu aaagaaugga gucuaaccuu uaacucagau      840

gauuuugacg acaaaauaga aggcuugucu gcgcaaauga cagaugaagc cggagaaauu      900

cuauuaaucc uuaaagaacu auacuucgca uuaaaucuaa gcgacaucuu aaacaacuca      960

gugacugaua aaaaugcaaa aacccuaucu gaagccauga uuaauagaua ugaugaccac     1020

aaagaacagu uaacaauguu aaaacaagug aucgauaauu cagacaagaa acaugcuaaa     1080

gcacucaaag cugcuuacuc cgcauacauu gauggagaaa auaauaagaa aauuagcuuu     1140

gaggaucucc gaaaacgaau ccagaaaaau uuagauaauu cagaaacauc aaagaaaauc     1200

aacgaccucc uagaacacga uuacuuucuu ccuaaacaaa gauccaaaga aaacggcgcc     1260

auaccacacc aacuccaaca acaagagcuc gauaaaauaa ucgaaucaca aaaacauuau     1320

uaucccuuuc ucgcugagcu caauccuaac gagaaaagau caagacaagc uaaauacaaa     1380

cuugacgaac ucguagcguu ucgcguucca uacuaugucg gacccauggu aaaagcaaca     1440

gaaaacaacc aaagcaauaa cggaaaauuu gcuuggauga uuaaaaauga caacauauca     1500

gauaaauuaa agaaagaaga aauaacucca uggaacuuug acaagaaagu agaccguacg     1560

gaaacagcaa caaaauucau aacccgaaug acgacaacag acacauaccu uauaggagaa     1620

ccaguacuuc ccaaaaacuc ccugcucuac caaaaauuug uaguauuaaa ugaacuaaau     1680

aacuuaaaaa uaaacaaaca accaauaaca ccagaacaaa aacaauacgu auacgaacac     1740

guuaugaaac aaaccaaaag aguaagucug aagaaaauug cagaugcacu cguuauuaaa     1800

ggagacuauc ccuacgcacc agaaauaacg ggaaccaccg aucagaaaaa caugaauaac     1860

ggauuaagua cauauauaga ccucgcaaaa auuuucggag acgaauuaga ugauaucgac     1920

agaaaagaag acuucgagaa aauuaucgaa uggucuacca uuuuugaaga ccaacacaua     1980

uucgcgcuaa aacucaaaga aaucaaaugg cucacugaag aucaaagaaa acaaguuguc     2040

aaacuaagau aucaaggcug gggaaaacua aguaagaaau uacuagccga uuuaguggac     2100

gauaacgguc aacgcauaau cgaccuacuu ugggacucaa aacaagauuu caugcaaauu     2160

guaaaccaaa auagcuucaa acaagcaaua gcagaacaca auggagcaca ucuugaagaa     2220

aaagaucuaa aagauaucau aaauaaccua uacacauccc cucaaaauaa gaaagcuaua     2280

cgacaaguua uacucguagu agacgacaua auccgagcag uagguuacga accgguaaau     2340

auaaugaugg aguucgcacg agaagacagu aaagaccaca ggcuaacaaa uaguagauca     2400

cgccaauuag aaaaagucua uaaagauaua acuaauucau gguucgacga ugauaacgua     2460

aaagacgaac uuaaagacaa aaucaaagau aaagccaaau uuacugacag auuauacuua     2520

uacuucacac aaggggguaa agaccuauau acaggagaac cccuagacau agacaauuua     2580

uccaacuacc aaauagauca uauacuucca caaucguuua uacuagacaa uucgcuagac     2640

aaccgagugc uaaccucuaa aauaaauaau caaauaaaga aagacaaucu accaggcgaa     2700

uuuuucgggu caaaaaugca uaacuucugg aaacacuuaa aagaacuuca ccuaaucucu     2760

aacaagaaau acuacaaucu aacgcuaaca cccaacagua uaucuaaauu caauaaucaa     2820

gguagauuua uuaaccgaca acucguagaa acucgacaag uuauaaaacu agcagcagaa     2880

auccuccaca auagauacuc agaagaaaau ggcacaaaca ucgucacagu uaaagcgaac     2940

cucacacacc auaugagaga aaaauucaac uuuuacaaaa auagaaacgu caacgauuac     3000

caucaugccu uugacgccua ccucgcagca uuuguaggua auuggcuauu acaacaauac     3060

ccaaaacuca aacccuacuu cguauauggu gacuucgcua aaacagaaau ugauaaccua     3120

aaauccuuua acuuaauaua caaauuugaa caagcacauc aacuuaacga agaagacggu     3180

aaaauugcca aagauacaga ugcacuccua ggcuacauga aaaacgucua ccauuuuaag     3240

aaaaugcuug uaacaaaaga acuggaaacc aaucacggua acuuauucaa acaaacccua     3300

uacccgagcc augguaaaau gaaaaaccuc aucaacccaa aagaaaaccg cucaucugac     3360

auauaugggg gcuacucaag cgaaacaggc gccuauguuu cacuaauuaa agucuuuaag     3420

aaaaacaaaa uagaaaacaa aauaauaaaa auaccacuac uauacaucga uaaacugaac     3480

cgccaagcaa acgcaaaaca auuuauacgc aauuucauaa uaaaagacga cuccaacaaa     3540

uucgacauau uaagcgagaa aauauuuuua aaucagcuag uaauagacgg agaugaacuc     3600

uucacuauau ccggaucagg agaucgacaa aaugcacaac aacuaauacu aucagacaaa     3660

ucucuuaaaa uucuauccaa aaacauaaac guaauaucca aaaaugaucu uauaauaaua     3720

uucaacgaaa uuaucgauga uauauguaaa uacaugaaaa uguacgaagu agaaaacuca     3780

gacgaaacua aagcaaaauu cgaagaaauc aacgauaaag accgaauguu aaaaauaauc     3840

aaugaccucu uauuaggccu ucaugccaau gccucaagau caaaccuuuc guccauagga     3900

uugucuccau cauucggcag auuuuucuca ggaaacgcag gaauaaaauu auccgaaaac     3960

gccaaauuua uuuaccaaag cccaacagga uuauuuucuc gaaaagugaa aauaucagac     4020

cuuuga                                                                4026


<210>  5313
<211>  4032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5313
augacauauu auuuaggauu agacauggga acaucgucag uagguugggc agugacugac       60

gaaaauuaca auuuaauaca caggcaucgg aaggaucuau ggggaauaag ggaguucgaa      120

gaagcuaaga cagcaguaga acgacguaca caaagaauau caagaagacg aagacagaga      180

gaaguagcgc gaauaggacu auuaaaagau uacuuucaug acgagauaca aaaaguagau      240

ccaaauuuuu accaacgacu agacaauagu aaauacuucc cggaagauaa agaagaggua      300

gucaaaggca aaaauggaau auucgcagau gaaaacuaca cugacagaga cuauuucgca      360

gaauauccaa cgauauucca uuuaaggagg gaauuguuga auagcaaaga gccgcaugau      420

guaagauuau uauaccucgc cuuacucaau auguauaaac acagagggca cuuuuuaaau      480

accacacuag guaccgacgg agaaaacaga aaacuuucag auguauacag agauuuugug      540

gucaaaacug aggaaguucu cggacuuaca uucccacagg aaacugauaa cggaaaaaug      600

caagagauaa uguguucgag agaguucucg agaaagagaa aagcagaauu acuaugcgcg      660

cuacuagaaa uagauaaaaa gcaaaaacaa caaguagcau uaauugaaug cguauuagga      720

uuagauaagu caguuaaaca aauguacggu acagcagucc cugaugauuu ugacaaaagc      780

guaaaaguca auuuuuccga caguucauuc gaagaaaaag ucagugaagu ugaagaacug      840

cucgaugagg agaaauauga auuaauuuug uguaugaaag aacuguacga uacagcaguc      900

uuaaaucaaa uaaugggaga uuguaauuau cucagcgaag ccaggguaca agaauaugag      960

aaacauaaag aggaccugaa auugcugaag aggacaguaa agaaauaugg aacugcagaa     1020

gaauaccaau uucuuuucag aagcucagag aaggcuuccu acaacgcaua uguccauucu     1080

guaaauucag gcgaaaagau uagacgaaac auggauggaa gauccagaga cgaucucuau     1140

acaguacuca agaaaaugcu caaaaauuau ccacaggaag augccgacug uaaauauauu     1200

cuagaagaaa uagaaaaaga acaauuuuua ccaaaacaau uagucuccga uaacggcguu     1260

auuccgaacc aaauacacua uaaagaaaug aagaaaaucu uagaaaacgc cgaacaauac     1320

uuacauuucu uacgagaaaa agacgaaagu ggcuacacug ucgccgaacg aauauuaaaa     1380

uuauuugcuu uucaaauucc auacuacaua ggacccacca gcgaaaaauc agggaaaaac     1440

gguggaaacg guuggguagu aagaaaagaa caaggucaag uuuuaccaug gaauaucgaa     1500

gagaaaauug auaugaaagc aacuucagaa accuucauau caagaauggu aaggcguugu     1560

acauaucuca gugacgagau ggucuuacca aaagccucau uggaauacga aucauucugu     1620

guguugaaug agcuaaauaa ccuaagaaua agaggaguaa aacuaccaau aacaguaaaa     1680

caagacauau auaaagauuu auuugagaga gggaaaagag uaaccagaaa acaaauagua     1740

aacuaucuua aagguguagg aauuuuacaa gaaggugaag aagacgaacu uaccgguaug     1800

auggacaccu uuaacucauc acuagcuuca uacgguaaau ucaaaucaau acuaggcgag     1860

aaaauacaag aagacaguug caaaagaaug guugaacaaa ucauauuuug guguacaaua     1920

uauggcgacg caaagaaauu ccuaaaagaa caacuugaag aaaaauaugg agauguccua     1980

accagagaac aaauaaaaag aauuuuagga uuuaaacuaa aagacugggg aaaccuuucu     2040

aagaaauuuc uagaacuaaa uggaugcgac cgaaauacag gugaaguaau gucacuaaua     2100

cgaaugcuau gggaaagcga cgacaacuua auggagcuuu uaaaagacuc aagcuauaca     2160

uaucgagccg aauuagaaga acaacaagaa aaacuuacca aaacucucac agaaauaugu     2220

ccagaagacc uagaagguua uuauuuuuca gcacccguca aacggaugau auggcaaaca     2280

cuauugauuc ugaaagaagu cgaaaaaguu augggagagc ccccgaaaag aguauuuguu     2340

gaaaugacgc gggaacauga ugaacgcagg gaaaugaaag auucgagagg gaagaaauuc     2400

caagaauugu acaaaaacau aaaagaugaa gaacacaacu ggaaagcgga aauagaagau     2460

gccgagaaag acggaaggau cagaagcaag aaaauguauu uauaucuaac ucagagaggg     2520

cgaugcaugu acacaggaaa uccuauagau cuagaagaau uauucacaac caaauacgac     2580

auagaccaua uuuacccacg acacuucguu aaagacgaca auuuagacaa uaauuuagua     2640

cuuguggaga aagagaaaaa ugcacacaaa ucagaccaau auccucuagg cgaugacaua     2700

uacacggguc gauugagcug guggaaagaa uuacacagag aaaaauuaau caacgacgaa     2760

aaauauaaac gccuuacagg gagagcacca uuuucagaug aacaaaaagc aggauuuauu     2820

gcaagacagc uaguagaaac aucacaagga accaaaggag ucgcaucaau acuuaaagaa     2880

guauuaccag aaacgacaac aauagucuac gcuaaagcaa gaaacguauc ugauuucaga     2940

aacaccaacg aauuacuaaa agcgcgauca guaaaugauu uccaucacgc acaagacgca     3000

uauuuaaaca uagugguagg uaacgucuau uauguaaaau uuacaaauaa cccucucaau     3060

uuuguaagaa gcggugaagg auacaaucua gaugguguau uucgcaaaaa ugucauaaga     3120

ggccaagaaa augcauggau aguaaaacca gagaaaggag agaaaaugac acuggaaacu     3180

gucaaaagag ugauggcuaa aaauacgccc uuauuaacua ggcuaucguu ugaaggacgu     3240

ggaggauuag caaaccaaac acucuacuca gcaaaagaag cagcaggcga agguuacgua     3300

ccauuaaaaa guagugauuc aagacuacaa gacguaacua aauacggagg auuuacuucg     3360

gugacuaccg cauacuuuau ucuaguagag caugauauga aaaagaaacg cgucagaacu     3420

auagaaagcg uucccauacu uuggaaagag cggauagaaa aagaaucugg ccaacuagaa     3480

auuuauugcc gagaggcauu aggccuagua aacccagaca uaaggguucg aaaaauacug     3540

auucaaucau uaguaaagaa agauggauuu uacaugcacc uaagcggaaa aacagguaac     3600

cgauucauaa ugagaaacgc gguagaauua uguuugaaac aagaauggau aaacuauagu     3660

aaaaagcuag aaaaagaagg augugaugag ucaaucacaa aagagaaaaa cgaagaauua     3720

uaugaaauuu ugaaggagaa acauauuaac ggaauauaca gaaaaaggcc aaacccaaug     3780

ggagagaaac ugcacauaag acaagaaacg uuuagaaugu uaacuuuaga ggaacaaaag     3840

aaaguacuac ucaguuuacu caaucuaaca aaaauaggac cugucgaagc caaccuggaa     3900

uugauagggg aaucagcaca caccggcaaa augcuaauaa gcaaaaacau aacagaagua     3960

aaggaauuuu acuuaauuca ucaaaguguu acaggcauau augaaaagca aguaaaccua     4020

cuaacgguau ga                                                         4032


<210>  5314
<211>  4032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5314
auggcuacca aaaaggaaua caacauaggu cucgauaucg gaacuacuuc uguuggcugg       60

aguguaguag aaacagacaa ucaaaaagua augagaaaag gaaauaaggc auuaugggga      120

guaagacuau uugaggaagc cacaacugca gagucaagaa gaaugcaacg aagcacaagg      180

cggagguaug auagacgaag agaacgcauu aaacuacuuc aagaggaauu uucggaagaa      240

auaaacaaag ucgacaagaa cuucuuucaa aaacucaagg agaguaagua uguagaaaau      300

gauaaaauaa auaaaaaaau cguacuaaca aaagaagaaa aacaggaauu aaaggacuau      360

caaaauaaau auaaaacaau cuaucaccua agagaugaac ucauaaacaa uccugaaaaa      420

aaagacauua ggcucguaua ccuagcaaua caucauauaa ucaaauauag aggaaacuuu      480

cucuaucaaa augccaacuu uaauauagac aaccuagaca uaaaggacaa gcuaaaugaa      540

cuauuuucca uccuaagcaa caacauacaa gaauuagaaa uaccagaaga cuacauaaac      600

auuauugacu ucgacgaacu agaagcugac uuacuaaagg acaccaagaa cgaugucaag      660

gcucuacuca uagauaaucu aaccgaauua acuaauaaaa gcagcgcgac agaauucggu      720

aaauuaaugg uaggaaauaa aggaaauaua aacaaauuau uaaugcuaga aacagacaac      780

aaaaucgaga uaucauuuuc aggcacagac uacgaugaca aauacgaaga auaccaagaa      840

gcacuaggag aaaauauaga aaucuuagau auauugaaac aaauauauga cuguauuuuc      900

cucaagaaaa uauucaaagg aaacaaaaac acuagcauaa guucacuaau gguaaaauca      960

uuugaacagc acaagaaaga uuuaaacuuc cuuaaagaac uauuuaaaga caacagggaa     1020

cuguacaacg aaauauucag aacaaaaaag gaucuauguu uauacgaaaa auauaucacg     1080

aauaaaauag auugcgauga auucagaaag gaaauaaaca aauugcuaga aucacuguuc     1140

agugacgaaa acauaaaaca aaaucuccuc gaugaauaca accuugaagu caaagaacga     1200

auagaaaacg guacuuucuu accuagaaua acuacaaccg auaauggcaa auaccccuac     1260

caacucaaca aaucagagcu aauaaaaaua auagaaaacc aaggcaaaua cuauccauuc     1320

cuacuaaaua aacuaaauga caacaagacu uauaaaauag uaaaauuacu ggaauucaga     1380

auacccuacu acgugggacc acuaguuuca gaaaaaagau cagaaaauag cuggcuagaa     1440

agaaaaauca auaacguaaa aauaacccca uauaauuucg augacguagu agacaaagaa     1500

aaaacagcag aaaaguuuau uaagagaaug auaagucacu guacguaccu ucuagacgaa     1560

uacgcauugg caaacaacag cauauuauac agcgaauuca aaguaaugaa cgaauugaaa     1620

caaauaagag uaaacgacag aaaauuagaa cuaaaauucc aacaacaaau acuagaagac     1680

uucuuuaaaa agacauccgg uacaauaacu gaacggaaau ucaaaaacua uuuaaucagc     1740

acaggggaau acucaaugga agguggcaac uucagaauaa caggcuacuc agcugacgga     1800

aaauucgcaa auaacaugca aucauaugua gauuuuuucg gugaaaacgg aauauucgcg     1860

gauacagacu auaaccuaga aaaugcagaa gaaauuauag aguggauaac aauauucgaa     1920

gacaaagaca uacuugaaaa aaaagucaga gacaacuacu caaaacuaaa cgaaaaucaa     1980

auuaaaaaag ugcuagcaaa gaaauacuca ggauggggau cacucucaaa aaaauuauug     2040

aaaacuaaau acuauaaaga uaaagcaaca gaacuauaua aaaguauuuu agaucucaug     2100

uacgagaccg acaaaaauuu caugcaaaua auaaacgacg acaaauauaa cuuccaagaa     2160

augaucaaaa aauuuaacaa caaggaggaa aauaaaaaac uaaacuacuc cauugucgaa     2220

gaacucgcaa caaguccagc uacgaaaaga gguauuuacc aagcauuaaa aguaguagaa     2280

gaauugguag acuacauagg auacgaacca aaaaauauau caauagaaau ggcaagauca     2340

gaagaagaaa aaguuagaaa agauagcaga aaagacuaua uaaauaaucu auacaaaggu     2400

ugcaaagauu cuauagaaaa cuauaaaaag cuuaagcaug agcuagacag ucaugagauc     2460

acaucacagc gauuauuucu auacuucauc caggaaggaa aaugcuuaua uacagggaca     2520

ccacugaaca uugaggacau cgaaaaccaa ucauuauacg aaauagacca cauaauacca     2580

agaucucuua uaaaagauga cucuauagac aacaaagcuu uaguacuuaa agaauguaau     2640

caagauaaaa aggcaucaua uguauugcca ucaaaauuua ggaacagcaa acaaaaaacc     2700

ugguggaaac auuugaagga caacggauua augucggcaa aaaaauuuca uaaucugaua     2760

agagaaaaau acaacgagga agacauaaac ggguuuauca acagacaauu aguagaaaca     2820

agacaaauaa cuaagcaugu agcgaauaua cucaauaauc uauacaaaga uacuaacgua     2880

auauaucuga aagcagauuu aucgcacaac uacagagaaa aauacgacuu auucaaauuc     2940

agagauauaa augacuacca ccacgcacac gacgccuauc uggcggccgu acuuggagaa     3000

uacaaagaac gcuucaugaa gagaaaaaua aacuucgaca ugguaaagga auugaacagu     3060

aaaauaguag aaauggauga aggaaaaagg agaaaccuuc guuucggcua cgucaucaau     3120

agucuagaug aaaacuuaaa cgauauaguc cuuaaaaucu cagaaaaccu cguagauaau     3180

gaaaccggag aaguccucuu ugaugcacac gaauuuaaca aacgaguuga agacacccua     3240

uacagaaaug acauacuaau aucacgaaaa acagaaauuc guacaggcca guuuuauaaa     3300

gaaacaauau auaaaaaagg uaaggggaca auaccaauaa aauucggaau gccuacagac     3360

auauauggcg gcuacucaaa uacuaaugca aggaaacuca ugcuaauaga auauaaugag     3420

ucuaaaauga aaauuauagg acuaccgaug gcacuugcgg uuagaaacaa cgaugcagaa     3480

auaaacgacu acaucaagac acaaaucaac cuuaagaaaa acaguaccuu uaagauuaaa     3540

aaauguagca ucccuuucga aacagaaaua auuuacaaca aucaaaaugu auucauaaaa     3600

gguuacagca uuggucacaa aaauugcgaa aucucaaaug caauacaacu uaaaauagcc     3660

aaagauaaaa ugaaacauug gaaauaugca cuacaauacc uauuaaacga caaaaaacaa     3720

uacaucgacu ucgcagagaa auauguaaau gaaauauacg acuuccuaau aaaccuagau     3780

cuauucccgc uauucaauaa agaauuagac aaaauuaaaa acaaagugaa cuuugaagau     3840

cuagcacucg accaacaaaa aaaaauuacu aaagaacuau uuaaaaugcu acauugcaac     3900

ucaguaaacg cgaaccucuc agaauucggc cuuggagaca gaaugggacg acucucagga     3960

uacaacauua cagauggaau cauuaucuca aaaucaguaa caggaauaag ggaauccaga     4020

uacgaguucu ga                                                         4032


<210>  5315
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5315
augagccaaa acaacaauaa aauuuacaau auaggacucg auauaggaga cgccagugug       60

ggaugggcag uaguugauga acacuacaac cucuuaaaac gucauggaaa acacaugugg      120

ggcagccggc uauuuacaca ggccaacacu gcuguggaac gaagaucauc aagaagcacu      180

cgacgaagau auaauaaacg aagagaaaga auccgacugu uacgagaaau aauggaagau      240

augguuuuag augucgaccc aacuuucuuc auaagauuag caaacguauc auuuuuagau      300

caagaagaca agaaagauua ucuuaaagaa aauuaccaca guaauuauaa ucuauuuauu      360

gacaaagauu ucaacgacaa aacuuauuac gauaaauauc caacaauaua ccacuuaaga      420

aaacauuuau gcgaaaguaa agaaaaagaa gacccaagac uaaucuaucu agcccuacac      480

cauauaguaa aauaucgggg aaauuuucuu uacgaagguc aaaaauucuc aauggacgua      540

ucuaacauag aagauaaaau gauagacgua cuccgacaau ucaacgaaau uaaccuauuu      600

gaauacgucg aagauagaaa gaaaauagac gaaguauuaa acguuuuaaa agaaccacua      660

aguaagaaac acaaagcaga aaaagcauuc gcucuauucg acacgacgaa agauaauaaa      720

gcugcauaua aagaacugug cgcagcacua gcaggcaaca aauucaacgu aacgaaaaug      780

cuaaaagaag cagaacucca cgaugaagau gaaaaagaua ucagcuuuaa auucagcgac      840

gcaacguucg acgaugcauu uguagaaaaa caaccacuuu uaggcgauug cguagaauuu      900

auagaucuau uacaugacau auacaguugg gucgaacugc aaaacauacu aggaagcgca      960

cacacaagcg aaccaagcau aagcgcugca augauccagc gauacgaaga ccacaaaaac     1020

gauuuaaaac ugcuaaaaga cguaauacga aaauaccucc caaagaaaua cuucgaagug     1080

uuuagagaug aaaaauccaa gaaaaauaau uacugcaacu acaucaacca uccgucuaaa     1140

acaccaguug acgaguucua uaaauauauc aagaaacuaa uagagaaaau agaugacccc     1200

gacguuaaaa caauauugaa uaaaaucgaa cuggaaaguu uuaugcuuaa acaaaauucg     1260

cgaacaaacg gagcuguccc auaucaaaug cagcuagaug aauugaacaa aauacuagaa     1320

aaucaaagcg uauauuauuc agaucuaaaa gauaacgaag acaaaauaag aucaauauua     1380

acguucagaa uaccauauua uuucggucca uuaaauauaa caaaagaccg acaauucgau     1440

uggauuauca agaaagaagg uaaagaaaau gaaagaauac uaccauggaa ugcaaaugaa     1500

auaguagacg ucgacaaaac ugcugaugag uucauuaaaa gaaugagaaa cuucuguaca     1560

uacuuccccg acgaaccagu aauggcuaaa aauaguuuaa caguaagcaa auaugaagua     1620

cuuaacgaaa uuaacaaacu caggaucaac gaucaucuca uaaaacguga caugaaagac     1680

aaaaugcuuc auacucuuuu uauggaucau aaaaguauau cagcaaaugc aaugaagaaa     1740

uggcuaguca aaaaccaaua uuuuucuaac acagaugaca uuaaaauuga aggauuucaa     1800

aaagaaaaug caugcucaac aagucuaacc ccauggauag auuucacuaa aaucuuugga     1860

aaaauaaacg aaaguaauua ugacuuuaua gagaaaauua ucuacgacgu uacgguauuc     1920

gaagacaaga aaauacuacg aagaagacuc aagaaagaau acgauuugga cgaagagaaa     1980

aucaagaaaa uucuaaaauu gaaauacagc ggguggagcc gacuuagcaa gaaacuacug     2040

agcggaauca aaacaaaaua caaagauagu acuagaacuc ccgaaacagu auuagaagua     2100

auggaaagaa caaauaugaa ccucaugcaa guaaucaacg acgaaaaacu uggauucaag     2160

aaaacaaucg augacgccaa cucaacauca guaucaggua aauucucaua ugcagaagua     2220

caagaauuag caggcucucc agcaauuaaa agaggaaucu ggcaagcacu acuaauaguu     2280

gacgaaauaa agaaaauaau gaaacacgaa cccgcacacg uauauauaga auuugcaaga     2340

aaugaagaug aaaaagaaag aaaagauagu uucgucaacc aaaugcugaa acuauacaaa     2400

gacuacgauu ucgaagauga aacugaaaaa gaagcgaaua aacauuuaaa aggcgaagau     2460

gcuaaaucca aaauuagaag cgaaaggcuu aaacuauacu acacacaaau gggaaaaugc     2520

auguacacag gaaaaaguuu agacaucgau cgacuggaca cauaccaagu agaucacaua     2580

guaccccaau cccuauuaaa agacgauuca auagacaaua aagugcuagu acuaucuagu     2640

gaaaaucaaa gaaaacuaga ugacuuagua auuccaagca gcauaagaaa caaaauguau     2700

ggcuucuggg aaaaacuauu uaauaacaaa aucaucucgc ccaagaaauu uuauucacua     2760

auaaaaaccg aguucaacga aaaagaucaa gaaagauuca uaaauagaca gauuguagaa     2820

acaagacaaa uaacaaagca uguagcacaa auaauagaua accacuauga aaacacaaaa     2880

gucguaacag uacgggcaga cuuaagucau caguucagag aacgcuauca uauauacaaa     2940

aaccgcgaca uaaacgacuu ccaccacgcc cacgaugcau auauagcaac aauacucgga     3000

acauauauag gacaccgcuu ugaaucguua gacgccaagu acauuuaugg agaauauaaa     3060

agaauuuuua ggaaccagaa aaauaagggc aaagagauga agaaaaacaa ugacggauuu     3120

auacuaaacu caaugagaaa caucuaugcc gacaaagaca cgggugaaau cgucugggau     3180

ccuaauuaua uugacagaau aaagaaaugu uucuacuaca aagauugcuu cguaaccaag     3240

aaacuagaag agaauaaugg uacauucuuu aaugugacag uccugccaaa ugauacuaac     3300

ucagacaaag auaauacauu agcaacaguc ccaguuaaca aauaccgcuc aaacguaaau     3360

aaauacggcg gauuuucagg ugucaacuca uucaucgucg caaucaaagg caaaaagaag     3420

aaaggcaaga aagucaucga aguaaauaaa uuaacaggca uuccauuaau guacaaaaac     3480

gcugaugaag aaauaaaaau caacuaucua aaacaagcag aagacuuaga agaaguacaa     3540

aucggaaaag agauccuaaa gaaucaauua auagaaaaag acggaggcuu auauuacaua     3600

guagcgccaa cagaaauuau aaaugcgaaa caacuaauau uaaaugaauc ccaaacaaaa     3660

uuaguaugug aaaucuacaa agcaaugaaa uauaaaaacu acgauaauuu agauucagag     3720

aaaauuauag auuuauauag auuacuaaua aacaaaaugg agcuauacua cccagaauau     3780

cgaaaacaac ucgucaaaaa guuugaggau agauaugaac aacuaaaagu aauaucaaua     3840

gaagaaaaau gcaauauaau caagcaaaua cuugcaacac uacacuguaa cagcucaauc     3900

gggaaaauaa uguacucaga uuucaagauu aguaccacaa uaggaagauu aaaugguagg     3960

accauuaguu uagacgacau aaguuuuaua gcagaaagcc caaccgggau guacagcaag     4020

aaauauaaac uauga                                                      4035


<210>  5316
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5316
augaagaaaa caaacccaua cauacuauca cuugacauug gaacagcuuc cauaggguac       60

gcaugcaugg accuagacuu caauauauua aaauaccacg acaaagacgc aaucggcguu      120

cuaacauucg aaggagcuga uacagcagca gaaagaagag guuuuagaac gaguagacga      180

aggaaaaaca gacgaauaaa aaggcuagga cuauugcaag aaguucuugc uccauuaguc      240

aaaaacccaa acuacuauca acuacuaaac cuucauaaau ggaaaaacuc caacgaagac      300

uucaaacaca gaucacugag ugaaguucuc aaaauacugg guaaaaaccc aaaaacguau      360

ccgacaauau aucaccuaca acaagacaua caaaaucacc cagagaagaa auucgaaccg      420

gaacuaauau uuuuagcauu guaccauuua gugaaauaca gagggcacuu cuuauucaac      480

aaucuauccu ucgauaaagg aaaaacagua gacaaugaag cccaacuuuc ugaacuccua      540

gagcaauacc aaucuauaaa uccguacgaa gauucaauau caaauaaaga uauuaacgug      600

aucguggaca cccuuaaaga cgaccaacua acacgaaacg acaaaaguaa agaacuuaaa      660

aggaauguua guaaaaccca ugcagaacug uuuaaaaugc ucguagggcu aaaauucaaa      720

caaagcauac uauucgaaaa cauggaaaau gcugaagaua uaaaagaauc agcaacaggg      780

uccgaugcca uaucacuaag cgacgacuac uaugaaaauc uaucagaguu cuuaacagac      840

gaacaacuua auuucauaga auccgcaaau acauucuuua ugggaauaau gcuagaucgu      900

auccuuaaag gagcaaccag cauauccgca gcuaaaguac gagacuacaa aauauuuaaa      960

aaccaacuaa aaauuaucaa agacauagua uaccaaaaca aauacgacaa auaucacgaa     1020

uuuuucguua cauccaaaga agacaugaga gcauaucaag aaaauccgga cgcaaaaacc     1080

uuuggaaaau uaaguaaauu ugaccaauau cuuauacacc cuaaagauca auacgcaaaa     1140

cuaaugaaag aucuaaaagg aaucgugccc aagaaauaua cggaagugcu aaauuuaauc     1200

gacgagcaga aauuucuugu aguacaaaau acaacagaua augcaagcau acccuaucaa     1260

aauaacguau ucgaagccga aaauaucuug aggaaucaac aaaaauacua uccagagaua     1320

acugaagaaa ugaucgggaa aguaaucaau cuaaucaguu uccgaauacc uuacuaugua     1380

ggaccccuga caaacaaaaa cggacaaagu gacuucgcau ggaugauaag aaaaagugac     1440

gaacaaacac uaccauggaa cuucgauaaa guaaucgaca aaucaaaauc ugcagaaaaa     1500

uucauaagac gaaugaccuc aaaaugcaca uauuuauuag aagaagacgu ucuaccgaaa     1560

cacagccucc ucuaucaaga aauggaagua cuaaacgaau uaaauaaugu acaauuacgg     1620

ggcgagaacg agcccaaaca ucgaauacac cgucuagauc ccaaaguaaa acaauuuaua     1680

uaucacaauc uauuucagaa aaagaaaaau guuacacaca aagaccucau cacguuauua     1740

caaaauucag aacacagaaa cacucuuaac aaaacuucgg gacaaaaauu aaaagucuuc     1800

ggaacacaaa augauucaaa auuugccuca aaauugagua cuuacaauga uaugaaagcc     1860

auauucgguu caguugaaaa caaaagacaa augauagaag aacuuguacu auggauaacc     1920

auuuucgagg acaaagcaau ucuaaaacag aaaauaggag aaaaauaccc aaauauaaca     1980

gaaaaacaac uaaaucaaau aguaaaacua aauuacagcg gauggggucg cuuaagcgaa     2040

aaacuauuaa ccaaagaagu aaauggacga ucguuaaucg accuaaugua cuccacagaa     2100

aacaacuuca uggaaauaau caccgauaaa acuuuaggau ucgauugcuu caucaaaaac     2160

agcaauaaag ucaaaaacgc gaaaauuucu uacaaagacg uagcagaauu gagaacaucg     2220

ccagcucuca agaaaggaau cuggaaugcc auaaaaaugg uacgcgaaau aagcgauuua     2280

uucggagcac cagagaaaau aauaauugaa uuugccacag aagaccaagu aaaaggaaaa     2340

agacaaaaau cccgaucuga acucugggac gaccuuguca agaaaaauaa cuuaaaacga     2400

aacaagaaau ucaaaggcuu auuugaagaa cuuaaagccu acccagaccu agauuuuucu     2460

aauccaaaau uauggcuaua cauacaccaa aaugguaagu gcauguauac caagaaaccu     2520

auagauuuag aacgguuaau gucagauaca aacaaucagc uauaugaaau agaccauauc     2580

cuaccacgga cuuuuguaaa agacgauuca auaaauaaua aaguacucgu aauccaaagc     2640

gcaaaccaga agaaaucagg agacaaaaug ccucucgaaa uauucgauaa ccacgugcua     2700

guacccuuuu ggaaaauguu auaugaaaac gaucuaauau cauccuccaa acugucuaaa     2760

cuuaugaaga aagaguucaa ugaccuagac aaagaaggau uuauucaaag acagcuagug     2820

gagacacgac aaauaauuuc acacguaagu gacuucuuaa cagaagagua cccaaguaca     2880

acgguuguac ccaugaaggc uaacguaguu ucugaauuaa gaaggaaauu ugacauccca     2940

aaaaucagac aacuaaacga uuggcaucau gcgguugaug ccuaccugaa cggauucgua     3000

uaccaagcag caagagucuu cuacccaaag guaaaccuau uugaguucaa uuucaaaugg     3060

gagaaaguca gagaaaaaug gcgccaacua ggagaguuca ccgaaaauaa caagaaaaga     3120

gaguucuucc uauucaaaag aauggaaaau uauguaaacg aacacggaga agggaugauu     3180

uccaaauuaa uuuucgaccu ugauaauuuc aaguucaacu acucaagaaa aguggaaagc     3240

acugaaucgg cuuucuacaa acaaucggca uuuagcccca aaaacacaac agcacaauac     3300

gauucggaca aaaauaaaac agcaauauau aaagaaauga aaaacaaaag aggaaucgua     3360

guauccuaca cauacguaaa gaaagaccaa gaacuaauag gauacggagu uuuuaacgaa     3420

acuguauucg aauaugucaa caacgcagac guagacauga auacaagagc auuaaagaua     3480

guacgaaagg cuuuuccaaa auuagacaua aagaaagcac aauacuuauu uucgauuaac     3540

aaaggagacg cauuacucgu aaacggacau ccauuucugu uuguaucagc aaaagaacga     3600

auuaacggaa agcaacuaga guuugacaua aauaugcaaa gaaggcucaa gaaagccuug     3660

cgaccauaua acaaaaugac cgaacaagaa aaaguaggag uuuauaauca auuugcagaa     3720

aaaguugucc aagaauauga aaacagccuu ggugcaaaaa caagaaacga aaaauuagaa     3780

uuaauacgaa aucaauucaa cgagagugac aaaucuaaug aagcacucau ccaguguauu     3840

cuugaagucc ucaagaugac caaagucucu gcagaacgau caguuaaacu aaaaauaaga     3900

ugggggucac aaacaccuaa uacacuaaua gacauaaaca auaaagcaaa aauaauguau     3960

aauagcauaa ccggccucaa gcaaacacga cuaaaaucaa uauauaagcu agccgaaagc     4020

aaaaacgaac uuuga                                                      4035


<210>  5317
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5317
augaauaaaa auuacuacau uggacuagac auggguacaa auucaguugg uugggcugua       60

acagaucaag aauacaaccu aauuaaauua aaaggaacuc augcaugggg agcuagaaua      120

uuucaagaag caaaagacaa aaaagguacc agagcaaucc gaucuagccg uaggagaaug      180

gacaggagaa aauaccgcau acacuuacuu aaucaacuau ucgcacaaga gauacaagcc      240

aucgacagua ccuucuucau aagacucgca uauuccgcau auugggaagu cgacaaaaaa      300

gacaaaggaa ucggacgaaa uauacuguuu aaaacaaaac aagaagaauc ucaauucuac      360

aagaaauacc cuacaauaug gcaccuacga aaaagccuuc uugaaaacca gccagaugcu      420

uucucugauc uuagauacgu cuaucucgcc uuccaccaca ucaugaaaua caggggcaac      480

uuucuccaug aaggggagau uaaaacaaau gaacuacagg uacagcaaau cgaacaacuc      540

aaucaauuuu uaauaaacaa auauaaagag auauacgaca augauguaga agaaagaacu      600

uucauagcaa accaccagau agaugcacua aaagaaauac uauuaaacaa aaacacaaau      660

aaaacuacaa aagagaaaga aauuaaaaaa cuauucgacu acgcagauug ugaaagcuua      720

gaaccauaua ucucguguuu cagcaaacuc gccguuggag gaaaauuuga ucuuuccaaa      780

cugggauaug aaggcaauau ucaauucuca gacccgucau uugaagagaa agccucaauc      840

cuucaagacg auuuccgucu aauugcuaua gcaaaagaaa uguacgauuu ugucauacua      900

aacgauuuau ugaaggauca acccaaucua ucaacugcca ugguacacgu guacgagacu      960

caccgacaag accucaaaaa auuaaaagac auaauaauug auauugacgc uaacaaaggu     1020

cuccaaaaca augaucguug guauuuuaaa cuauucaaag auaaaaacgc accuaaaaac     1080

uauuccgcau ucauucacaa aaacaguaau caaaaacgac caagcauaga ugaauucaac     1140

gacgaaauca aaaaaauacu caucgaacac agaaacgacg uaucacuuga aaaacaagau     1200

gacuuaaaaa uguuauuuga aaaaguagaa aauaagacuc ucuuaaaaac aauagccaua     1260

caaaguacau caguuauacc ccaccaacua caccucgaug aacuugaaaa aaucauagau     1320

aacgcaucua ccaaauaccc auuucuauca aacauaagag acaaacucgu auuguuauuu     1380

acauucaggg uaccauauua uuauggucca cuaaaugaua aaagccccua uucaaaugua     1440

gagagaaaac aguucgccac aguuacgcca uggaacuucg acgagauauu ugaugaaaac     1500

aaaacacgag aaaaauucau gaaaaaacuc acuaacaacu gcaccuacuu aagcggcgaa     1560

acugucaugc caaaaaaggc ccuagccuac gaagauuacc uaauauacga uagacuaaau     1620

guccuacuag uaaacggaga auaccuaucu cuacaagaaa aagaaaaaau auaccacuac     1680

cucauaucaa gacccaaaac cacacucgac caauugaaaa aagaacuagc uaaauggcac     1740

aaccaaucac caauugacau caucuucagc aacauacacg aagaaauucc auucgaagca     1800

ucaucgcacg cacaccucau gaaaucauuu gaucucgaaa agcaaaaaga agaauuagaa     1860

agguacauaa cucuagccac uauauacgca gaugacaaaa agucccucaa agaacuacuc     1920

aaaaacgaau cuuuacucga cgagaaccaa aagaauguaa ucuuaacccu cccaacuaaa     1980

aagugggcac cacuaaguua uaaacuacuc cagaauaucg uauacacaga cgacuauggu     2040

guaguucacu caauccucga cauaaugaaa gaaacaaacc aaaauuucca aaugguacuc     2100

cauaacgagc aauaccacuu uaaagaacua auugaacagg aaaaccagaa auucacacaa     2160

gaaaaaacga ucgaagaccu aauagaagaa aaauuagaau caauaccuuc ucuaacccaa     2220

cgcaguauca gacaaacacu acucaucaua gacgauauaa ucaaagccag ccaccaaaaa     2280

guaccagaga aaauauuuau agaagucacc agaaacaacg augauaaacg aaaaggucaa     2340

gacgguaauc aauuagcacg cgauaaagaa uugaaccguu uuuuaacaaa cuuacucaaa     2400

gacgaaaaag caaaccaauu cugcgacguc ucacaacuau uaaaggacuu agaagaaguc     2460

gauaaaguaa aauuaaaagg aaagcacaua uaucucuacu uuaaacaauu aggcauagau     2520

uuguauaccg gaaaaaaaau aguccuaaac caacuccuaa ccucuacaaa auacgacaca     2580

gaccacauaa uaccccaguc uuuaauaaaa gacgauucuc uagacaaccu aguuuuagua     2640

gaaagagagg uuaaccuaaa aguaaaagga aaucacuauc ccguuccauc acgaauccag     2700

gaugaacaaa aaaaacucug gcacuuccuu cuccaaaaaa aggcgauaag cgagaaaaaa     2760

uacggaaauc ucacuagaac aucaaaaauu aagcucgaag aagucgaaga cuucgucgcu     2820

agacaaauua acgucaucaa uuauuccaac guuauaauaa gagacauacu caaacuaaaa     2880

uacccacaca ccgacauaau auucucaaag ucccaauacc caucauucau aagaaagcac     2940

cuuaaaaucg ucaaaaauag agacguaaac gacgcacacc augcagucga cgccuaucua     3000

aacguaguau caggaaaucu auuaucaaca acuuuuucaa aagcuuauau cagaaaaaaa     3060

uauauccaaa uacaggaugg cgaaaaagaa acauucaaca uggagacaau ucucgaaaau     3120

uacuuuaaaa acaaugaaaa ccaacuaaag auaaaaacaa auugccuuag acgagacgcu     3180

uuaauaaccu uuaagguaga cuacuucagc ggugaguuuu acggacaaac aagacuacca     3240

aaaggaaaug aaaaucugau acccauaaca cuaaacgaag auaaccccau gucagacacu     3300

ucaaaauaug gugguuauac caacuuagcg caagcuuacc uauuugcagu uucguaucaa     3360

gaagacggag aaaugaaaaa agcaauacua agaguaccau aucuauauga cacacuauac     3420

aaaaaugauu cggauaaacu acuuucaaaa guucuugaca aucccaaagc aacaaacauu     3480

caaauccuca gaaagauaca ccuaaaccaa aaaauccguu uuaacgaugg uauauauuua     3540

uuauauacaa augaaaaaga cagaaacaaa uauaaaaugg cguaucaaaa cuauauagac     3600

aacgauuacu uauacuaccu ccacugggca aacaaacauu uagaagaacu uuuagauaau     3660

caggagaaac aaaccuuuau auauaacucc aaaaacgauc aauuugaagu aucaaaagca     3720

aaaaacaaag aaaucuuuca auaccuucuc caaaaaacaa aggcuaaagu cuaugacaca     3780

ugcaacuaua uaguaaaagc acgagaaacu aacauagaaa ucuucgagca auuaacauua     3840

aaagaacaaa uaacgaugau caaccaaugg auuaagauga ugucuagaga cugcgaaaag     3900

guuaaauucg augcaagguu uacuaauuuu ucaaacugcc uucuacgccu cucaaauaac     3960

auaaacccaa aagacagcau aaccauuauu uaugaaucac cuaccggauu auucaagcac     4020

gaaguaaaaa uauga                                                      4035


<210>  5318
<211>  4035
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5318
augaagaaaa ccaauccaua uauauuauca uuagacauag gaacagccag uauuggcuac       60

gcauguaugg accuagacuu caacauauua aaauaucacg acaaagaugc aauaggugua      120

cuaacauucg aaggagcaga cacagcagca gaaagaagag guuucagaac gagcagacga      180

aggaaaaauc gaagaauaaa acgccucggc uuauuacaag aaguacuugc cccacugguc      240

aaaaauccaa acuacuauca acuacuaaac cuacacaaau ggaaaaacuc caacgaagac      300

uuuaaacacc gcucacucuc ggaaguacuc aaaaccuugg gcaaaaaccc aaaaacauac      360

ccaaccauau aucaucugca acaagauaua caaaaucacc cggagaagaa auucgaacca      420

gaauugauau uccuagcgcu uuaucaccua guaaaauaua gaggccacuu uuuauuuaau      480

aaucuuucau ucgacaaagg aaaaaccgua gacaaugaag cucaacuuuc cgaauuacuu      540

gaacaauacc aaucaauaaa cccauacgaa ggaucaauau uuaacaaaga caucaaaguu      600

auaguagaua cauuaaaaga cgaucaacuc acgagaaaug auaaaagcaa agaacugaaa      660

cgaaauguua guaaaacuca ugcugaacua uucaaaaugu uagucggucu uaaguucaaa      720

caaucaauac uauucgaaaa uauggaaaac guagaagaca uuaaagaaag ugccaccgga      780

agcgacgcaa uaagucucuc agacgacuau uaugaaaacu uaucagaauu ucucacagac      840

gaacaauuaa auuuuauaga aucagccaac accuucuuua ugggaauaau guuagaccgc      900

auccuaaaag gcgcuaccag cauaucagca gcuaaaguac gggauuacaa aauuuucaaa      960

aaccaauuaa aacucaucaa agacauagua uaucaaaaua aauacgacaa auaucacgag     1020

uucuuuguaa caagcaaaga agauaugcga gccuaccaag aaaauccaga cgcaaaaaca     1080

uuuggaaaac uaucaaaauu cgaccaauau cucauucacc caaaagauca auaugcaaaa     1140

cucaugaaag acuuaaaagg aaucguuccc aagaaauaua ccgaaguucu uaaucuaauu     1200

gacgagcaaa aauuccuugu aguacaaaau acaacagaca augcaucaau acccuaucaa     1260

aacaauguau ucgaagcaga aaauauucug agaaaucaac aaaaauauua cccugaaauu     1320

acagaagaaa ugaucggaaa aguaauaaac cuuauuuccu uccgcauucc uuauuacgua     1380

gggccccuca cuaauaaaaa uggccaauca gauuuugcau ggaugauacg caaaucggau     1440

gaacaagcac uacccuggaa uuuugacaaa guaauagaua aaagcaaauc agcugaaaaa     1500

uucaucagac gaaugacaag caaaugcaca uaccuuuuag aagaagacgu cuuaccaaaa     1560

cacucauuac uuuaccaaga aauggaagua cuaaacgaac uaaauaacgu ucaauuacgu     1620

ggggaaaacg aaccaaaaca caggauucac cgccucgacc caaaaguaaa acaauuuauc     1680

uaccacaauc uauuccagaa aaagaaaaac guaacacaca aagaccuuau uacacuacug     1740

caaaacucag agcauagaaa uacacuaaac aaaacguccg gacagaaacu gaaagucuuu     1800

ggaacccaaa acgauucaaa auucgcauca aaacuaucca cauauaauga uaugaaagca     1860

auuuucggau caguugaaaa uaaaagacaa augauagaag aacucguucu auggguaacc     1920

aucuuugaag acaaagcaau acucaaacag aaaauaggcg aaaaauaucc uaacauaacc     1980

gaaaaacaac ucaaccaaau cgugaaauua aacuacucag gauggggaag auuaucugaa     2040

aaauuacuaa cgaaagaagu aaauggccgc uccuuaauag accuaaugua uuccaccgaa     2100

aauaacuuca uggaaauaau aacagauaaa accuuaggau ucgacuguuu uaucaaaaac     2160

ucaaauaaag ucaaaaacgc gaaaauaucu uauaaagaug uugcagaacu ccgaaccagu     2220

ccggcauuaa agaaaggaau auggaaugca auuaaaaugg uccgcgaaau aagcaauuua     2280

uucggagccc cagagaaaau aauaauagaa uuugcaacug aagaccaagu aaaaggaaaa     2340

agacagaaau cacguaguga acuuugggau gaccucguca agaaaaauaa ucucaaacgg     2400

aacaaagagu ucaaaggauu auuugaagaa aucaaggcuu auccagaucu ggauuuuucc     2460

aacccgaaau uauggcucua cauucaucaa aacggcaaau guauguacac caagaaacca     2520

auugacuuag aacgacuacu uucugacacg aauaaccaac uauacgaaau agaccauauc     2580

uuaccuagaa ccuucguuaa ggacgauucc auaaauaaca aagucuuagu cauacaaucu     2640

gccaaccaga agaaaucagg agacaaaaug ccccuugaaa uauucgauaa ucauguccuu     2700

gucccauucu ggaaaaugcu uuacgagaau gauuuaauau ccucuagcaa acucucuaaa     2760

cuaaugaaga aagaguucaa cgaucuugau aaagaaggau uuauucaacg ccagcuagua     2820

gaaacuagac aaauaaucuc acacguauca gacuuccuaa cagaagaaua uccuucuacg     2880

accgucguac ccaugaaagc caacguagug uccgaacuca gaaggaaauu cgacauaccu     2940

aaaauacgac aauuaaauga uuggcaccau gccguagaug ccuaccuaaa cggauuugua     3000

uaccaggcag cacgaguuuu cuacccaaag guaaaccuau uugaauuuaa uuuuaaaugg     3060

gagaaaguac gagagaaaug gagacaacua ggagaguuca cugaaaacaa caagaaaaga     3120

gaguucuuuu uauuuaaaag aauggaaaac uacguuaacg aacaugguga aggaaugaua     3180

agcaaacuaa ucuucgaccu ugacaacuuu aaauucaauu acaguagaaa aguagaaucc     3240

acggaaucag ccuucuacaa acaaagcgca uucucuccga aaaauacgac ugcccaauau     3300

gacucugaca aaaauaaaac ggcgauauac aaagaaauga aaaauaaacg cggcauagug     3360

guuaguuaca cauacgucaa gaaagaucaa gaacuuauag gcuacggcgu auucaacgaa     3420

acaguauuug aguacguaaa caaugcagac guagacauga acacgagagc ccucaaaaua     3480

guaaggaagg ccuuucccaa acuugacaua aagaaaguac aguacuuauu cucgaucaac     3540

aaaggcgaca cgcuccuagu aaacggacac cccuuccuau ucguaagcgc auaugaaaga     3600

auaaacggca aacaguuaga auuugacaua gcuaugcaaa gaaaaauaaa acaagccuua     3660

aguccauaca acaauaugac agaacaagaa aaaguugaau uuuacaacca auucgcggaa     3720

aaaguuauag aagaauuuag aaauuccauu ggacuacaaa guagagaccg gaaaguuaau     3780

cugaucaaaa accuuuucaa caauacagaa aaauccaacg auuccaugac ccaaagcauc     3840

uuagaaguau uaaaaaugac gaaagucucg gcagaaaggu caaaagaacu caaaauaaga     3900

uggaacggac aaaccccgaa cacuuucauu gacacuaaaa acaacgcgga aauacaauau     3960

aaaucaauca cuggacugaa aacaacaaaa ccaaagagua uauucaaacu agccgaaagc     4020

aaaaacgagc uuuga                                                      4035


<210>  5319
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5319
augaaaaaac cauauagcau cgguuuggac auaggcacaa acucgguagg augggcugua       60

guaacagacg acuauaaagu acccgccaag aaaaugaaag uacuaggaaa cacugacaaa      120

ucacauauaa aaaagaaucu acuaggagcu cuauuauucg auucuggaaa uacagccgaa      180

gaccgccgac ugaaaagaac agcaaggagg cgauacacaa gaagaagaaa uagaauccua      240

uaccuacaag aaaucuuuuc cgaagaaaug ggaaaaguag acgacucauu cuuccauaga      300

cuugaagauu cauuuuuggu aacagaagau aaacgcggcg aacggcaccc aaucuucgga      360

aaucuagaag aggaagucaa auaucaugaa aauuucccaa caauauacca ccuacgacag      420

uacuuagcag auaaucccga aaaaaccgac uuaagguuag uauaucuggc gcucgcacac      480

auaauaaaau ucggaggaca uuuuuuaaua gaagguaaau uugacacaag aaauaaugac      540

guacaaagau uauuccaaga auucuuagca guauacgaca acacauucga aaacagcuca      600

cuacaagaac aaaacgucca aguggaagaa auacucacug acaaaauauc aaaaagcgca      660

aaaaaagaca gaguacuaaa acuauucccu aaugaaaagu caaacggaag auucgcagag      720

uuccuuaaac ugauagucgg uaaucaggca gacuucaaga aacacuucga auuagaagaa      780

aaagcaccac uacaauucuc caaagauaua uacgaagaag aacucgaagu auuacucgcc      840

caaauuggag acaacuacgc agaauuguuc uuaucugcca aaaaauuaua ugacuccaua      900

cuucuuagug gaauucucac agugacggac guaucuacca aagcaccacu aagcgcgagc      960

augauacaac gcuauaauga acaucaaaug gacuuagcac aacucaaaca guuuauacgc     1020

caaaaauuaa gcgauaaaua uaacgaaguu uuuucugacg uuucuaaaga uggauaugca     1080

ggauacaucg auggcaaaac aaaccaagaa gccuucuaua aauaccuuaa aggacuacua     1140

aauaaaaucg aagguagugg uuacuucuua gauaaaauag aacgggaaga uuucuugaga     1200

aaacaacgua cauucgacaa cgggagcauc ccccaccaaa uacauuuaca ggaaaugaga     1260

gcaaucauuc gccgacaagc agaauuuuac ccauuucucg cagauaauca agacagaaua     1320

gaaaaaaucc uaacguuuag aauaccauau uacguaggcc ccuuagcuag aggcaaaucc     1380

gacuucgcau ggcugucaag aaaaucagca gauaaaauca ccccauggaa uuucgaugaa     1440

auaguugaua aagaaucaag uguagaagca uuuauuaauc gaaugacaaa cuacgaccua     1500

uacuugccaa accagaaagu acuuccaaaa cacucccugc ucuaugaaaa auuuacuguu     1560

uauaaugaac ucacuaaagu gaaguacaaa acagaacaag gcaaaaccgc auucuucgau     1620

gccaacauga aacaagaaau auuugaugga guauucaaag uauaucgcaa agucaccaaa     1680

gacaaacuua uggauuucuu agaaaaggag uucgacgaau uucgaauagu cgaucuaaca     1740

ggucuugaua aagaaaauaa agcguuuaac gcuuccuaug gaacuuacca cgaucuacga     1800

aaaauauuag auaaagauuu cuuagacaac agcaaaaacg aaaaaauacu ggaagacauc     1860

guacuuacuc uaacucucuu cgaggacaga gaaaugauaa gaaagagauu aaaaaacuac     1920

ucugacuuac uaacaaagga acaacucaaa aaacuugaac gaagacacua cacagguugg     1980

gguagacucu ccgcagaacu gauacacggu auuagaaaca aagaaucaag aaaaacgauu     2040

uuagauuauc uaaucgacga uggaaauuca aacagaaauu uuaugcaacu cauuaaugac     2100

gaugcacucu ccuuuaaaga agaaauagcu aaagcacaag uuauaggaga aaccgauaau     2160

cuaaaucaag ucgugucuga caucgcuggu uccccagcaa uuaaaaaagg aauacuacaa     2220

ucgcuaaaaa uaguagacga acucguuaag auaaugggac accaaccaga gaacauagua     2280

guagagaugg ccagagaaaa ucaguucaca aaucaaggca gaagaaacuc acagcaacga     2340

uugaaaggau uaacugacuc aauaaaagaa uucggaaguc aaauacucaa agaacacccc     2400

guagaaaauu cucagcucca aaacgauaga cucuuucuuu acuaccuaca gaacggccgc     2460

gauauguaca caggagaaga acuagauauc gacuaccugu cucaauauga caucgaccac     2520

auaauuccgc aagcauucau uaaagauaac agcauagaca auagaguacu aaccucuucc     2580

aaggagaaca gaggaaaauc agacgacgua ccgucuaaaa auguaguuag aaaaaugaaa     2640

aguuauuggu cuaaguuacu aagcgcaaaa cuaaucaccc aaagaaaauu cgacaauuua     2700

accaaagccg aacgaggagg auuaacagau gacgacaagg caggguucau aaaaagacaa     2760

uuaguagaaa caagacaaau uacaaagcac guggcacgaa uauuagauga acgcuuccau     2820

acagaaaccg augagaacaa caaaaaaauc cgccaaguaa aaauagugac acugaaaucc     2880

aaccucguua gcaacuuccg aaaagaauuu gagcuauaca aagugagaga aauaaaugac     2940

uaucaccaug cccaugacgc cuacuuaaac gcaguaauag gaaaagcacu acuagguguc     3000

uauccacaau uagaaccaga guuugucuau ggagacuauc cacacuucca uggccauaaa     3060

gaaaauaagg cuacagccaa aaaauuuuuu uacucaaaca ucaugaacuu cuucaaaaaa     3120

gaugauguua ggaccgacaa aaacggugaa aucauaugga aaaaagauga acacauauca     3180

aacauaaaga agguauuauc uuacccacaa gucaacauag uaaaaaaagu cgaagaacaa     3240

accggcggau ucaguaaaga aucgauacuc ccaaaaggga acagugauaa acuaauacca     3300

cgaaaaacca aaaaauuuua uugggauacu aagaaauacg gaggauuuga caguccaauu     3360

gucgccuauu caaucuuagu uauagccgac auagaaaagg guaaauccaa aaaauugaaa     3420

accguaaaag cauuaguagg aguaacgaua auggagaaaa ugaccuuuga aagagaucca     3480

guugcauucc uugagagaaa aggauauaga aacguacaag aggagaacau aauuaaacuu     3540

ccgaaauaca guuuauucaa acuagaaaac ggaagaaaaa gauuacucgc aagcgcacga     3600

gaacuucaga aaggaaauga gauaguguua ccuaaccauu uaggaaccuu auuguaccac     3660

gccaaaaaua uccauaaagu agacgaaccc aaacauuuag auuacgucga caaacacaag     3720

gacgaauuua aagaacuguu agacguaguc aguaauuuua guaaaaaaua uacacuugcg     3780

gaaggcaacu uggaaaaaau aaaagaacuu uacgcacaaa acaauggcga agaccuaaaa     3840

gaguuagcau caucguucau aaauuuacua acauucacag cuaucggugc accugcuaca     3900

uuuaaauuuu uugacaaaaa cauugauaga aaacgauaca caucaacaac cgaaauuuua     3960

aacgcaacac uaauacauca aagcauuaca ggucuauacg aaacaagaau agacuuaagu     4020

aaauuaggag gcgacuga                                                   4038


<210>  5320
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5320
auggaaaauu acaacauugg acuggacaua ggaaccacaa guguaggcug ggcuguagug       60

aacgaugcaa cuaacaaagu aaugagaaaa ggcgguaaag cacucugggg aguacgauua      120

uuugacccag ccguaacugc cgaagauaga cgagcaaaaa gaggcacaag gcgaagguac      180

gauagacgcc gagaacgaau aaaacuauua caagacgaau uuaaagacga aaucaacaaa      240

guugacucaa acuucuuucu aaaacuggaa gaaagcaaau auacauccaa agacauuaaa      300

aauaaaacag uacuaauaag cauggaagaa gaaaaggcca uaaagaaaua uaaccaagaa      360

uacccaacaa uauaccaccu aagacaaaaa uuaaugaacu ccaacgaaca aauggauauc      420

agacuggugu aucucgcaau acaucacaua auuaaauaca gaggaaacuu uauauaugaa      480

gguaacuccu uuucgguaaa uaacuuaaac auagacaaua aauugaaaga aauauuuuua      540

ucgcuaucgg aagacguaga ugaacucgau auaucugaag aauguauuaa caauauaaac      600

uacaaacaau uagcaaacau auuuaacgaa caaaguaaaa acgaucuaaa aguaaaauua      660

acaagugaau uaacuagcua uucacugaau aaaaauuuca uauccgaguu cuguaaacua      720

auaauaggua acaaauucuc ggcuaagaaa uuauucaauc uugaaacaga caacgauguu      780

ucacuuucau uugacggaac cgauuuugaa gacaaagcag gagaccuuga aucacuacuc      840

aaugacuauc uagaaguauu agaacaacua aaagaacuau acgauauggu uuucuuaaag      900

aaaauauucg gaggcucaaa agaaacaaca auauccggac uaaugguaga aagauacaac      960

cagcauaaga aagacuuaaa acuccuaaaa gauacguuca guucugauag gaaaauauau     1020

aauaaaauau uuagaaguaa gaaagacauc aaagaugaua aagauaugug ccucuacgac     1080

cuauacauac acaauaaacu agaaacaaca gacuuccuua aagaaauuaa gaaauacgua     1140

aacaccgaua auuucaucau aacagaugaa gacuuccuaa aaagacuaga aaacaaugaa     1200

guacuaccaa gaauaacauc aaaagaaaac gguaaauauc ccuaucaacu uaauaaagac     1260

gaacucguau caauaauaga acaucaagga aaacacuacc ccuuucuacu uaacaaaaca     1320

gacgacggca uauauaaauu aguucgccuu uuagaauuua gaauacccua uuucguagga     1380

ccccuaguau caaaagauau gagugaaaau gcuuggaugc ucagaaacga agguaugcaa     1440

ggaguacaua uaacgccaua caacuuuaag aaaguaauaa auuuagauaa aacugcggaa     1500

aucuuuauaa aaagaaugau aagccauugu acauaucuac uugaugaaga agcacuacca     1560

aauaacucaa uccuauauuc ggaguucaaa guuuugaaug aacugaaaca aaucaaagua     1620

aauggcgaac accuuacgaa agacgaacaa cacaaaauaa uagaagaauu auucaugaaa     1680

acaucgggaa cgaucaccga aaauaaauuc agagaauuuc uacuuaacac acuagacuac     1740

aacuuuuacg aaaacgaacu caaaaucacc gguuauucag ccgacuguaa auucgcaaau     1800

aacaugcaaa guuacauaga cuucuucgga gaaaacggaa ucuucgaagg aacaucauau     1860

aauacaaaug acgcagagaa aaucauugaa uggauaacua uauucgauga uaaagauauc     1920

cucaaacgua aaguagaaaa cgaauaucca gaacuaaaaa guaaucaagu gaaaaacauc     1980

uuaaacaaga aauauucagg cuggggaagg uuaagcagaa aauuacuauu agaucuaaga     2040

guauuagaca aagaaacuga uauaccaaaa ucaaucaugg accucaugua cgaaacugau     2100

gagaauuuca ugcaaauaau aaacaaugac aaauauaacu uccaaaaacu aauugcuaaa     2160

gaaaacaaaa uugagaauac aagcaacgac uuaucguacg acguaguaga aaaucuagcc     2220

accagcccgg caacaaaaag agguaucuac caggcguuaa aaguugucaa agaaaucaca     2280

acauauaugg gcuacgaacc uaaaaacaua augguagaaa uggcuagagg agaugaaaag     2340

aaaauccgca aagacgauag aaagaaauau cuacaaaaau uauacgacaa cauaaaagac     2400

gaaguagauu caaacuacaa aaaucucaag aaagagcucg cgaaagaaga gaaaauagau     2460

accgacaaac ucuauuuaua uuauuuacag gaaggcaaau guuuauauug cgggaaacca     2520

auaaacguag augaccuaaa uaccaaaaac ucacuauacg aaauagauca cauauuacca     2580

agaacauuga ucaaagauaa cagcugggac aacagagugc uagugugccg aggcugcaac     2640

cagaagaaag cggccagucu aguacuccca aaagaguucc gaaacgauua uaccaaauca     2700

auauggauaa gacuuaaaaa caacaaacua augaguaaca agaaauucua caaccucacg     2760

agaaaaaguu auagcgaaga agauauaaaa ggauuuauaa auaggcaacu ugucgaaacg     2820

agacaaauaa caaaacaugu agccaacaua cucaauugcu ucuacccaaa aaccaaaaua     2880

gucuaccuca aagcuaauau aucgcauuca uacagagaua aauacgaccu auucaaauuc     2940

agagacauaa augacuauca ccacgcacau gaugcguacu uagcagccac acucgguagu     3000

uaucaagaaa aauaccuaca uuaugaccug gacuacaaua aaauuaauga guucaaugaa     3060

aaauugauua aaaaugaaga cuacaaacgg uuacauuaug ggauaguagu uaacagccuu     3120

gaugaagaau uucagucagc cuuugcagaa auugcaccac guuugauuaa cgaagaaaca     3180

ggugaauuac uauucaaugc caagaaauuu aaugaucucg ucgaagacac ucuauauaga     3240

aaugacauuu uaauaagcag gaaaacagaa aucagaaccg gcaaacuaua caaagaaaca     3300

auauacaaaa gggguaaagg uaacauaaaa auaaaagaaa auaugcccac ugaaacauau     3360

gguggcuacu caaacaugga aacaucauau uuaacgcuag ugaaauaugg uaauaaaaca     3420

aaacugauag gaauaccaau gucaauagca aacaacaaag auguaaaagu uaaacuagau     3480

uuuaucaaag aacaccuaga ccucaagucc cucgacaaca uagugauuuu aaaagauaau     3540

auaccguaug acgucucuau aaauuacaaa aaccaagacg uuuacgucaa aggauacuca     3600

acagccaaca aaaacuguga acuuuccaau gcacaccagc uuaaaauaaa gaaagauaug     3660

augaagaaau gcaaauacgu gcuuaacaaa guauauaaaa acaaauacga ugauauaaca     3720

aacauggaca uagaaaacgc uauaaccuuc auaaacuacu uauuugaaau aaaaauagag     3780

uucccacucu ucagcaaaga aauaacuaaa auacaagaca acaucaauuu aacagaaugu     3840

uccuucgaac aacuagccaa caucauuaaa caacugcuca uaaucuacca cugcaauagc     3900

aaaaacggaa aucuaaaaga auuuggacug agugauagaa ucgguaggcu uucaggagcu     3960

aacguaacag gcggggacuu cauauucacu ucagugacag gacucaaaga gaaaacugca     4020

uccuacgaug gagaauga                                                   4038


<210>  5321
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5321
auggucgaaa aauauggaau aggacuugau aucggaaccu caacacucgg auggacgagc       60

guagacgaaa acgguaaaau acaaagacua aaaggacaaa cagcacucgg aacaagauug      120

uuuaacgagg gccaaacagc agaagagaga aggggcuuuc guacaacaag gcguaggauu      180

caaagacgaa gauggagauu acgauuauug agagaaauau ucgacgaacc aauugguagu      240

guagaugcag gauucuuugc uagacagaaa augagcucuu ugucacccca agaugaucga      300

uucgaagaca cuuacagauu auuuaacaac caaaccgaca aggcuuucca ugacgaguac      360

cccacgacau aucaccuacg acaagcucua augaacucag accgucaauu ugauauuaga      420

gaaaucuacu uagcuaucca ccacauagua aaauacagag guaauuucuu aagaucagga      480

aaacccgccg acuuuaaaac aggcaaauua gaccuuaaag aaaaauucga agaaauaaau      540

gccgacuggc aaacauuaau aagugaagac gcuccacaac uuccaguuga ugauuuaacg      600

acaauaaaag caguauuagu cgaugguucg cguagucgua gcgaucgaca aagaggucuu      660

guaaaacucu ggauggccaa cgaaacuguc gagaaaaacu ggaaaccagu caugacugaa      720

uuagcaaaag caauacuagg cuuaaaaaca aaauuauauc ugcuaacagg acuagaucua      780

ccaaaagaag accaaaaagc auggacauuc ucacuggaua cacuugaaga ucaccaaggc      840

gacauagacu cauuuuucga ugaccaugcc caacaacuaa uagauagacu agcugaacua      900

agagcuauga uacaauuaac ggaaauuauc ccaagcggau uaaauuuuuc acaaagcaug      960

guucaacguu acgaagcaca uagaagucac cuggaaaugu acaaacaaua cgcucuagau     1020

caaacagauc ccaaacgcac aaaagcucua cgugcugcau acaacgcaua cauagaucaa     1080

cacgaaauag cagacgauuu uuacacagcc guuaaacgag uuaccaaaga cgaucaauca     1140

gaaacggcug aagaaauuaa uagauuaaua gggcuugaac aauucaugcc uaaacaaagg     1200

acaaaagaaa acggcguaau ucccuaucaa guacaucaac aagaacuaga ucaaauuaua     1260

gcucaccaaa agaaauacua cccuuuccua gggacagaaa acccaguaau agaccauaga     1320

gagagccaac ccuauauguu ggaugagcuu guaucauuua gaguaccaua cuacguggga     1380

ccuaugguug cagcaaaucc agaacuaaaa acaacugaaa auccguucgc auggaugaua     1440

agaaaagcuc aaggugagau aacgccuugg aacuucgaug aaaaaguaga cagaaaaggg     1500

ucagcaacag cguucauaga acgaaugaaa acaaccgaca cauauuuauu aggggaagau     1560

guauuaccuc aacaaagucu gcuuuaucaa agauuuaugg uauuaaauga auuaaauaau     1620

auaagaauag acgacgaacu auuaacaacc aaccaaaaac aauggguauu cgacagagua     1680

uucaaaggga agggaacgau auccgcuaaa cauuugcaag acgcacuaaa acaccauggu     1740

gaaacugacg ggcuuccgaa aaucucaggu cuagcggauc caacaaaguu uaauucagca     1800

cuuacaaguu accgagauuu ucaaaagugg uuacccaaug acauagacag aaaggaucgu     1860

acagcugaca uugaaaccau aauaaauugg aguacugucu uugaagacac ucacauauuu     1920

guagaaaaac uaaaagaaau agaauggcua acagcugauc aaagaagacg uauagcuagc     1980

aacagauacc gaggcugggg gagacucucu aaacaucuac uuaccgaaau aaaagaugag     2040

cagggacuau caauaaugga ccaacuuugg agaacccagc acaacuucau gcaaauagua     2100

cacgaaccag acuacgcaaa acaaauagag gcugcaaaua cagucggcau ggccaaacaa     2160

gauauuggaa caguaauaaa ugaucuauau accucuccac aaaauaagaa agcaauuagg     2220

caaguuuuag ccguagucaa agauauccaa aacgcccauc acggaguugc gccgaguugg     2280

auauuuaugg aagcagcgcg cggagcagau caaaauccac aacgaagcag acaaagacaa     2340

cgacaauuac aagaucuaua cgccaaaacu gcagccgauc ucgucgacac agaaguacaa     2400

caggaauuaa cugauaaaau caaggauaaa gcaaacuuua cggacagacu aauguuauac     2460

uucuuacaaa auggacagga cauguauucg aaagaaaaau uaaauauaga cagauuauca     2520

gaauaugaua uagaccacau acuuccucaa gcacuaauua aagaugacag ucucgacaac     2580

cgcguauugg uacaucaaag uauuaaucgu aacaaaucug auacauucgc aucagaaaaa     2640

uacggagcaa aaaugcaagg ggccuggcau caauggcgag cagcaggucu aauuucugca     2700

agaaaauuac gaaauuugga gaugagaccu gaugaaauca gcaaauaugc aacggguuuu     2760

auucguagac aauuaguaga aacacgacaa gucauaaaac uuguuacuga aauacuaucc     2820

caucaauaua auccagaaga aaccaaacuc gugucaguua aagcuggguu aagucaugag     2880

uuaagaagag aagcaggcuu acccaaacua cgagauuuaa acgacuauca ucacgccauu     2940

gaugcauauu uagcggcaag aauaggaaca uauuuacuga aacgauaucc acgauuagag     3000

agauucuuug uauacggaca auuucaaacg ucaaggcuag acuuaagacg uuuuaacuuc     3060

auacaugauu uaguuaaaag caaacaaggu gucauuguug auagcgauac aggugaaauc     3120

uuaugggaua gacaaaaaga uauagauuau aucaaacacc ucaugggucu caaacacuua     3180

cuuguaacuc augaaguaua ugacucagca gcggaaaugu acgcccaaac aauauucaag     3240

gccucggaua augacucaaa aguacucauu ccgacgaaaa augauagacc gacagccauu     3300

uacgguggau acacaaagag gagaaauggc uaccuagcua uacuuaaaau aacugagaag     3360

aaagccgaaa cguacagagu agucgguguu ccaacauugu acaugucuca acuagcaacc     3420

gcuagaucuc acucagugga agcugaaaaa gcacuucuaa agagcuugau ggcaccugag     3480

auucucuccg aaaguaaagu ugucucauuc gaagucguag ucucacaugu ccaucuuaau     3540

caacucguaa gagauaccgu uuucgguccc gcacauuauu ucggacuugg cccauucaga     3600

uacgucagaa auuuucauca gcuaguucua ccauuuaaag aucaagaaau cuugguuaaa     3660

agaacaccua cagaugaaga acuaaauacc guauucgaag acauauguug gcaagucgaa     3720

aauuacuucg caguuuuuga uauaaacgga uucagaaaug cauuaaagaa aaguauagac     3780

aaauucgaac aguuaccaau cgaaaaugug uuuaaagaua agaaauuagu cacucaaggc     3840

aagaaagaag uacuagcuuu ugugaugcaa ggacuacaug caaacgcuag uuaccgaaac     3900

cuuaaagucu cacucgguau aaaaacaccc cuaggacaau uacaauuaac aaaauucaug     3960

uuaucaagag augcagaauu aauagaugaa ucaccaaccg gauuauuucg acgaauuaga     4020

cgccuaaaag acuuauga                                                   4038


<210>  5322
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5322
auggguaaua aacuaaggaa uguagacgac uacuauuuag gccuagauau aggaacagaa       60

agcguaggau gggcugugac agauaaauau uacguaauuc caaaauucaa aggaaacgcc      120

auguggggag ugcaucucuu cgacgaaggg gaaacuucag ucaaacgaag aggcuucaga      180

agcgcaagaa ggagaacaca acgaaaaaga gaaagacucg cacuccucga agccuuauuu      240

gaugaggaaa uuugcaagaa agacugcacg uuuuucagaa gauuacauga auccgaccua      300

uggcccgaag acaaaagugg agacgacaaa uacgcagucu ucgcagauga gaauuacacc      360

gacaaagauu uucacaaaga auauccaaca auuuaucacu uacgcuacga auuaauuaaa      420

aacaaggaac cacacgacgu aaaacuugug uucuuagccc uacaucauau cauaaagaau      480

agaggacauu uucuauucga uagucucagu ucagcagaag acuuuaacag ugcauacgag      540

acgaugugca gcuacuuauc agacaaugaa auacaacuaa auugcagcga uauagaacag      600

uuuaaagcga uucuaaaaga uagaagacaa ggaaaaauag aaaagaccaa aaaucuagcg      660

ucauuauucg gaauaacaaa gaaauccgau ccggucucau acgcaguuau ggcuguacuu      720

agcggaucga caguuaaaau gugugaucuu uuuggagaug augaauuaaa aacgguagag      780

aaaaacaaua uaucauuugc uucaggauac gaagaaaaug aagaaauaua cgccagcgua      840

uuaggcgaca gguucgaauu aguaaaaauc cuuaaagccg uauaugacug ggcuguauua      900

gcagauaucu uagauggaaa gaaauauauc agcgaagcaa aaguagcagu cuacgaaaaa      960

cauaaaaacg accuaaaaac uuugaagaaa uauguaaaaa cuuauuuacc ugaaaaauac     1020

aaugaaauau uuaaaauauc agauggaaag aaagacaacu acguugcaua cagcggacau     1080

auaaaagaau cuggaaaaac cggagaacua aagaaaaccu gcaacucaga aaacuuuugc     1140

gcauauuuga agaaaacucu agguaaaaau gccgaccuau cauauaaaga aauguucgac     1200

gaaauugaaa auaaaacuuu caugccgaaa caagucacaa aaucaaaugg aguaguaccu     1260

augcaaguaa acgaagaaga acuaaaagcu auuuuagaua acgcaaaaga uuaucucucu     1320

uuccuaaaca guugugauga aaauggagug aguguguaug auaaaaucau caaaauauuu     1380

gaauacagaa uaccauauua ugucggaccu cuaaauacac acacugaaaa cagcugguua     1440

aaaagaaaag acgguguuaa aauauaucca uggaacuucg acgauguggu cgauauagac     1500

ucaucagcag aaaaauucau uaauaaccua acaucaaaau guacuuaucu cccugaaugu     1560

gaugucauac caaaaugcag uuuauuauau ucaaaauuua cgguucuuaa cgaaauaaac     1620

aauuuaaaac uuaauggaga agacauacca guugaauuga aacagggaau cuauaaagau     1680

cucuuuauga caagaagaaa aguaaccgua aaacagauaa aagauuaccu aaaggcuaga     1740

ggaugcuacg acaauaacga cguccucuca gguaucgaua caacuguuaa aucgucucuc     1800

aaaagcuacu uugauuucaa aggcuucgaa ucucuaaccu uugaugacaa agagucaauu     1860

auugcaucaa gcguaauauu cggggaugac aagaaacucc uaaagaaaag acucaagaaa     1920

cuuuuuccug agaaauuaac ugaugaggau aucaagaaaa uaugcaaacu aaaauauaca     1980

ggauggucaa ggcuaucuaa agaauuucug ugugauguug aauuuacuua caagaaaaca     2040

ggagaaguaa ugaacguaau aaccgccuua ugggaaacaa auuacaaccu uaugcaaauc     2100

uuguauagcg aagauuucug uugcgaugga gaugaaaaga aaaaccuaaa cgacaaauua     2160

gacgaauuau cauauaccgg agaccaacau agcuuaagaa aagcuguuga ugcacuauau     2220

guaucaccaa aaguaaaacg accgauucua agagccuuau uaauugcuaa agaaauagag     2280

aaaauacaag aaagaccacc caagaaaaua uuugucgaag ucgcuagagg aauugacaau     2340

cgagaagaaa ggcacaggga uucuaggaaa aaccgacuaa uggaacuaua caaaaauugu     2400

aagaaagaag cagaaaaauu cugccccuac uuugaugccc uaucaaagaa auuaucagac     2460

gaagacgaau caaaacuccg ccgagauaaa cuuuacuucu auuacauaca auuggguaaa     2520

ugcauguaua caggagaaga aauaagccua ggagaacuaa ugaacggaaa cgguagauuu     2580

gauauagacc auaucuaccc aagaucaaaa auuaaagaug acagccuuac caauaaaguc     2640

uuaguaaaua aaacuguaaa caacaacaaa acaaacguau acccaauaga cgcagcaaua     2700

caaucaaaaa ugagcccaua uuggcaaaug uuacgaagca gcgggcuaau aagcgaagaa     2760

acauauaaaa gacuuacucg aaaaacaccu uuaacagaug acgaauuaag ugcauucgua     2820

ucacgacaac uuaccgaaac ccaacaauca acuaaagcug uagcucacau ucuagaacaa     2880

cuauacccaa aaccaaaaac agagauugua uauguaaaag cagcccucgu acacgcauuc     2940

aggcagucga augacauguu aaaaugccgu gaaguuaaug acuuacacca ugcgaaagac     3000

gcuuaccuca acauaguagu aggaaacgca uauaauguaa aagucacgca uaacaaagug     3060

aacuuuauua aaggucuaca aaacaacgau aaaggcuaua cggucaaucc agaaaaauuu     3120

uucucucaug auauaccagg ugccugguca guuaaagauu caaugaauac uguacgaaga     3180

uuuaugaaua aaaacaauau cagguauaca agauuuccaa ggaagaaaca aggcggauua     3240

uuuaaaauua acccccucaa gaaaggaaau ggacaagucc cauuaaaggc uucuggagca     3300

cguagcugca uugaaaaaua uggcggauac gacaaagcaa caucagcaua uuuuaguuau     3360

gucgaauaua cagauaagaa aggaaaagca guaaggcaaa uguucccagu aaaccuauac     3420

gaaguaaaag aguaugaagc ggauaugcaa gguuucguga ccucuagaau agcugaagca     3480

aguaacguua aaaucuugau uccaugugua aaauaugaug ccugcauauc auuugacggu     3540

ugucgaaugu ucauaaccaa gaaaaccgga aauagaauug gauacaaacc uggaaugcaa     3600

cucguucuag gcuacaaaua ugaaaaauau auccguaaca ucgaaaacua cuugacuaaa     3660

cauaaagaca gagaaaucau accacgagac aacauaagcg cagaagaaaa uauugaacuc     3720

uaugauaucc uaaccggaaa aaugcuaaac accguauuau caguaaaauu uucuggaauu     3780

gggaagaaac uagagaaaag aagggaaaaa uucguguuau guagcccuga agaacaaugc     3840

ugcguauuaa acgaaauauu aaaaauccua caugcaaacg uacuaagcgg ugaccucagc     3900

cugauuggag aaaguccuaa caccggcaca augaaaacga acgcaaaauu cucagaaaua     3960

aaagauguac auagcauuaa acuaauaaau caaucaguaa cugguuuauu ugaacaagaa     4020

aucgacuuau uaaaauga                                                   4038


<210>  5323
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5323
augggcaaac cauauaguau aggcuuggac auagggacua acagcguugg uugggcagua       60

guaacagaug auuacaaagu gccagccaag aaaaugaaag uacucggcaa cacagauaaa      120

caaaguauca agaaaaaccu auuaggagca uuacuuuuug auucgggaga aacagcagaa      180

gcaacuagac uuaaaagaac agcaagacga agauacacaa gaaggaaaaa ccgucuaaga      240

uauuuacaag aaauauucac cggagaaaug aauaaaguag augaaaauuu cuuucaacga      300

cuugaugacu ccuuuuuagu ugacgaagac aaaagaggag aacaccaccc cauauuuggc      360

aauaucgcag ccgagguaaa guaccacgac gacuuuccaa ccauuuacca uuuaaggcgu      420

caccuugcag acacauccaa gaaagcagac uuaagguuag uauacuuggc auuagcacau      480

augauaaaau ucagaggcca cuuucuauac gaaggagauu uaaaagcuga aaauacggac      540

gugcaagccu uauuuaaaga cuucguggaa gaauacgaca aaacaauuga agaaagccac      600

uuaagcgaaa uaaccguaga ugcauuaucu aucuugacag aaaaaguauc aaaaucaucc      660

agacuggaaa acuuaaucgc ccauuacccg acagaaaaga aaaauacucu cuuugguaac      720

uuaaucgcac ucagcuuaga ucuacaucca aacuuuaaaa caaacuuuca acuuucggaa      780

gacgcaaaau uacaauucuc uaaagacaca uacgaagaag accuagaagg auuuuuaggc      840

gaaguaggug acgaauacgc agauuuauuu gcuagcgcca aaaaccuuua ugaugcaauc      900

cuauugucag gaauacuaac gguggacgac aauaguacca aagcaccacu auccgcaucu      960

augguaaaaa gguacgaaga acaccaaaaa gaccugaaga aacuaaaaga uuucauaaaa     1020

guaaacgccc ccgaccaaua caaugccaua uuuaaagaua aaaauaagaa agguuaugcu     1080

uccuacauag aauccggagu aaaacaagau gaauuuuaca aauauuuaaa aggaauauua     1140

cuuaaaauaa augguagcgg agauuuccua gauaaaauag acagagaaga cuuccuaaga     1200

aaacaacgua cuuuugacaa uggaucgaua ccccaucaaa uccaucucca agaaaugcac     1260

gcaauacuac gaagacaagg agaacacuau ccauuccuaa aagagaacca agacaaaaua     1320

gagaaaauac uuaccuuuag aauacccuau uacgucggcc cucuugcaag aaaaggcucc     1380

agauuugcau gggccgaaua uaaagcggac gagaaaauaa cucccuggaa cuucgaugac     1440

aucuuggaua aagagaaaag ugcagaaaaa uuuauaacaa gaaugacacu uaaugaucua     1500

uaucuuccag aagaaaaagu auuaccaaaa cauagcccgu uauacgaagc auuuacaguc     1560

uauaaugaac ucacaaaagu aaaauacguc aacgaacaag gagaagccaa auucuuugau     1620

accaauauga aacaagaaau auucgaccac guauuuaaag aaaaucgaaa aguaacgaaa     1680

gacaaacuuu uaaacuaucu aaacaaagaa uuugaagaau uuagaauugu uaaucuaacu     1740

ggccucgaca aagaaaacaa agccuucaac ucaucucuag gcacauacca ugacuuaagg     1800

aaaaucuuag acaaaucauu cuuagaugau aaagccaacg agaaaacaau agaagacaua     1860

auacaaacac uaacauuauu cgaagacaga gaaaugauaa gacaaagacu acaaaaauau     1920

ucagauauau ucacaaaagc acagcuuaag aaacuagaac gacgucauua cacaggaugg     1980

ggaagauuau cuuacaaauu aauaaacgga auuagaaaca aagaaaacaa gaaaacaauu     2040

cuagacuauu uaauugacga cggauaugca aacagaaacu ucaugcaacu aauaaacgac     2100

gacgcccuau cauuuaaaga agaaauagca cgugcacaaa uaaucgauga uguggaugac     2160

auagcaaaug ucguccacga ucucccagga ucaccagcaa ucaagaaagg aauacuccaa     2220

agcguuaaaa ucguagauga auuaguaaaa guaaugggac acaaucccgc aaacaucauu     2280

auagaaaugg cucgagaaaa ccaaaccacc gauaaaggaa gacggaacuc acaacaacga     2340

cucaaacuac uccaagacuc acuuaaaaac cuagauaauc ccgucaacau caaaaacgua     2400

gaaaaccaac agcuucaaaa ugaucgacua uuuuuauacu acauucaaaa uggaaaagac     2460

auguauacag gugaaacguu agacauuaau aaccucucgc aauaugauau ugaucacaua     2520

auuccgcagg cuuuuauaaa agauaacuca uuagauaaca gaguuuuaac cagaagugac     2580

aaaaacaggg gaaaaagcga cgacguacca aguauagaag uaguacacga aaugaaauca     2640

uuuuggagua aacuguuauc ugugaaauua aucacacaaa gaaaauucga uaaccuaacg     2700

aaagccgaac gaggcggacu aacagaagaa gauaaagcug guuucaucaa aagacaauua     2760

guagaaacaa gacaaauaac aaaacacgug gcacaaauuc uagacgaaag auucaacaca     2820

gaauuugaug gaaacaaacg aagaauacga aacguaaaaa ucauaacacu aaaaucgaau     2880

cuugugagca acuucagaaa agaguucgaa uuauauaaag uaagagaaau aaaugacuau     2940

caucacgcac acgaugccua ucuaaacgcc guuguaggaa augcauuauu auuaaaauau     3000

ccacagcucg aaccagaauu uguauacgga gaguacccua aauauaacuc uuaccgaagu     3060

cgaaaaucug caacagaaaa auuuuuauuu uauucuaaca uacucagauu uuucaagaaa     3120

gaagacauac aaacaaacga agauggggaa auugccugga acaaagaaaa acauauaaaa     3180

auacuuagaa aaguccuuuc auacccacaa guaaacaucg ucaagaaaac ggaagaacaa     3240

acaggaggcu uuucaaaaga gucaauacug cccaaagggg aaagcgauaa acucauccca     3300

cguaaaacca aaaauucaua uugggacccc aagaaauaug gaggauuuga cucaccggug     3360

guugcuuauu caauacuugu cuucgcagau gucgaaaaag guaaaagcaa gaaacuccgu     3420

aaaguacaag acaugguggg aaucacaaua auggagaaga aaagauucga gaaaaauccc     3480

guagacuucc uggaacaaag gggauaccga aauguuaggc uagagaaaau aauaaaauua     3540

ccaaaauaua gcuuauucga auuagaaaac aaaaggcgaa gacuacuagc cucagcuaaa     3600

gaauuacaaa aaggaaacga acucguaauc ccacaaagau ucacuacccu acuauaucau     3660

ucauauagaa uugaaaaaga uuacgaaccu gaacauaggg aauauguaga aaaacacaaa     3720

gacgaguuca aagaauuacu ggaguacaua ucagucuuua gcagaaaaua cguccuagcu     3780

gauaauaacu uaacaaaaau agaaaugcua uuuaguaaaa acaaagaugc ggagguaucu     3840

agcuuagcaa aauccuuuau uuccuuacua acuuuuacug cauucggcgc ucccgccgca     3900

uuuaauuucu ucggagaaaa cauagacaga aaaagauaua cuagcguuac agaaugccuc     3960

aaugcaaccu uaauacauca auccauaacu ggcuuauaug agaccaggau ugaccuauca     4020

aagcuaggcg aggauuga                                                   4038


<210>  5324
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5324
auggaucaaa aauacagcau aggcuuagau auagguacca auucaguagg cugggcugua       60

guuacagaug acuacaaagu gccagccaag aaaaugaaag ucuuaggaaa cacagauaaa      120

caaagcauca agaaaaauuu acuaggagcu cugcuauucg auagcggaga aacagcagaa      180

gcaacuagac uaaaaagaac agcacgaagg cgcuauacaa gacgucgaaa ucgacuaaga      240

uauuuacaag aaauauuugc agaagaaaug aauaaagugg acgaaaacuu cuuucaaaga      300

cuagaugacu cauuucuagu cgacgaagau aaacgagggg aacgccaccc aauauucgga      360

aauauagcag cagaaguaaa auaccacgac gauuucccaa ccauauauca cuuaagaaaa      420

caucuagcag acauaucaca aaaagcagac cuaaggcuag uauacuuagc auuagcacau      480

augauaaagu uuagaggcca cuuccuaaua gaaggacaau uaaaagcaga aaauaccaac      540

guacaagcau uauuuaaaga cuucgucgaa guauaugaca aaaccguaga agaaucccau      600

uuaagcgaaa ugaccguaga ugcacuuucu auucuaacag aaaaaguauc aaagucaaga      660

cgacuagaaa accuaauagc acacuaccca gcagagaaga aaaauacauu auuuggaaau      720

cuuauugcau uaucauuggg cuugcaaccu aacuucaaaa ccaacuuuca auugucagaa      780

gaugcuaaac ugcaauuuuc aaaagauacu uacgaagaag accuggaagg auuacuuggu      840

gaaauaggag augaauacgc ugaccuauuc gcaagcgcua aaaauuuaua cgaugcgaua      900

cuucuaucag ggauacuaac aguagaugac aacaguacga aagcaccacu uucagcuagc      960

augguaaaaa gauaugaaga acaccaaaag gaucuaaaga agcucaagga cuucauuaaa     1020

gugaacgcac cugaucaaua caaugcaauu uucaaagaca aaaauaagaa aggguacgcc     1080

ggauauauug aaaauggagu gaagcaagac gaauuuuaca aauaucuaaa aggaauacua     1140

uuacaaauua acggaucagg agauuuucuu gauaagauag accgagaaga uuucuugcga     1200

aaacaaagaa cauucgacaa cggaucuaua ccacaccaaa uucacuuaca agaaaugcau     1260

gcaauccuua gacgccaaga agaacacuac ccauucuuaa aagaaaauca agacaagauc     1320

gagaaaauuc ucacauuccg aaucccuuac uacguagggc cauuagcuag aaaaggauca     1380

cguuucgcau gggcggaaua uaaagcagac gagaaaauua caccauggaa uuucgacgau     1440

auacuagaca aagagaaaag cgccgaaaaa uuuauaacua gaaugacacu aaaugaccua     1500

uaucucccag aagaaaaagu gcuaccaaag cacuccuuau uauaugaaac auucacagua     1560

uacaacgaac uaaccaaagu gaaauauguc aaugaacaag gagaagcaaa auuuuucgau     1620

gcaaauauga aacaagaaau auucgaucac guauucaaag aaaauaggaa ggucacaaaa     1680

gauaaauuac uuaauuaccu aaacaaagaa uuugaagaau uuagaauugu aaacuuaaca     1740

ggccuugaua aagaaaauaa ggcauuuaac uccaguuugg guaccuauca cgaccucaga     1800

aaaauacucg auaaaucauu ucuugaugau aaggcaaacg agaaaacaau ugaagacauu     1860

auacaaaccc uuacccuauu ugaagaucga gaaaugauaa gacaacgauu gcaaaaauau     1920

ucagacauau ucacaaaagc ucagcucaag aaauuagaaa gacgacacua uaccggcugg     1980

ggacgauuau cauacaaguu aauuaauggc auccggaaua aagaaaacaa gaaaacaauc     2040

cucgauuauc uaauugauga cggauaugcg aauagaaauu uuaugcaguu aaucaaugac     2100

gaugcacucu cauuuaaaga agaaauagca agggcucaaa uuauugggga cgucgacgac     2160

auugcaaacg uaguacacga cuuaccaggc ucgccagcga ucaagaaagg uauacugcag     2220

ucaguaaaaa uagucgauga acuuguaaaa gugaugggac acaaucccgc aaacauaaua     2280

auagaaaugg cucgagaaaa ccaaaugaca gauaaaggaa gaaggaacag ccagcaaaga     2340

cuaaaacuau uacaagacag ccucaaaaau cuugauaauc caguaaauau uaaaaauguu     2400

gaaaaccagc aacugcaaaa cgaccgauua uuuuuauauu acauucaaaa uggaaaagac     2460

auguacacgg gugaaacgcu cgacauaaau aaucuaucgc aauaugacau agaucacauu     2520

auaccacagg cuuuuauaaa agauaauuca cuagacaacc gcgucuuaac aagauccgau     2580

aaaaauaggg gaaaaucaga ugacguacca ucaauagaag uaguacauga aaugaaaagu     2640

uucuggucca aacuguuauc aguaaaauua auaacucaga gaaaauucga caaccuaacu     2700

aaagcagaaa gaggaggccu cacagaagaa gacaaagccg gcuucauuaa acgacagcuu     2760

guggaaacac gacaaauaac uaaacaugua gcacaaauau uagacgaacg cuuuaauacu     2820

gaguucgaug gaaacaaacg ccgaauacga aauguaaaaa uaauuacacu caaauccaau     2880

uuggucucca acuuccgcaa agaguucgaa cuuuauaaag ucagagaaau caaugacuac     2940

caccacgcac augacgcaua cuuaaaugcu guaguuggaa augcucuacu acuaaaauac     3000

ccacaacuug aaccugaguu cguuuauggc gaauauccaa aauauaauuc auauagaagc     3060

cgcaaaucag caacagaaaa auuccuguuu uauucaaaua uccuuagauu cuucaagaaa     3120

gaagauauac aaacgaacga agauggagaa aucgcaugga auaaagaaaa acauauuaag     3180

auauuaagaa aaguacucuc auacccacaa guaaacaucg ucaagaaaac cgaagaacaa     3240

accggaggau uuaguaagga aucaauacuu ccaaaaggag aaagugacaa auuaauccca     3300

agaaaaacca aaaacucaua cuggaauccu aagaaauacg gcggauuuga cagccccguc     3360

guagcauauu caauccuagu uuucgcagau guagaaaaag gcaaaucaaa gaaauuaaga     3420

aaaguccaag auaugguagg aauuacaaua auggagaaga aaagauuuga aaaacaccca     3480

guagacuuuu uagaacaacg uggauacaga aacguaagac uagagaaaau aauaaaacug     3540

ccaaaauaca gucuguuuga acuagaaaac aaacgacgac gccuccuagc uucugcacga     3600

gaacuccaaa aaggcaacga auuagucauc ccacaaagau uuacaacucu acuauaccau     3660

uccuaccaga uugagaaaaa uuacgaaccc gaacaucgag aauauguaga aaaacacaaa     3720

gaugaguuca aggaauuacu agaauacauc aguguauuua gcagaaaaua cgucuuagca     3780

gacaacaacc uuacaaaaau agaaaugcua uuuuccaaga auaaagacgc agaaguuucc     3840

ucucuagcua aauccuucau cagcuuauua acuuucacug cauucggagc uccagcugca     3900

uuuaauuucu ucggagaaaa cauagacaga aaacgcuaca caucagucac ggaauguuua     3960

aacgcaacac uaauacacca auccauaacu ggacuauaug aaaccagaau agacuuaucg     4020

aaauuagggg aagauuga                                                   4038


<210>  5325
<211>  4038
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5325
augaagaaag aauacucaau agggcuagau auaggaacaa auucaguagg augggcagua       60

auaaccgacg auuauaaagu uccagcgaag aaaaugaaag uuuuaggcaa cacagacaaa      120

caaaguauca agaaaaaccu acuaggagcc cuacuauuug acagcgguga aacagcagaa      180

gcaacccgcc uaaaacggac ugcaaggcgu agauauacga ggaggaaaaa cagacugcgu      240

uaucuucagg aaaucuuuac cgaggaaaug aacaaaguug acgaaaacuu cuuccaaaga      300

cuagacgauu cauuucuagu ugaagaagau aaacaaggcu caaaauaccc aauauucggu      360

acguuaaaag aagaaaagga auaucacaag aaauucaaaa caauauacca ccuccgagaa      420

gaacuagcca auagcaaaga aaaagcagau uugcgucucg uauaucuugc acuugcucac      480

augauaaaau ucagagggca uuuucucuac gaaggugacu uaaaagccga aaacacggau      540

guacaagcuc uauucaaaga uuucguagaa gaauacgaua aaacaauaga agaaucacau      600

uuaucagaaa uaacuguaga cgcauuaagc auauuaacag aaaaaguaag uaaauccucc      660

agacucgaaa aucuaauagc acauuaccca acagagaaga aaaacacccu auuuggaaac      720

cuaauugcac uaucccuagg cuuacaacca aacuucaaaa ccaauuucca acuauccgaa      780

gacgcaaaau uacaauuuag caaagauacc uacgaagaag auuuagaaga acuccuagga      840

gaaauugggg augaauacgc agaccucuuu gcgagugcua aaaaccucua ugaugccaua      900

cugcuauccg gcauauuagc aguagacgac aacacuacca aagcaccacu aagcgcaagc      960

augguaaaac gcuacgaaga acaucaaaaa gaucucaaga aauuaaaaga uuucauaaaa     1020

gucaacgccc ccgaucaaua caacgcaauc uuuaaagaua aaaacaagaa aggauaugca     1080

ggauauauug aaucgggagu agaacaagac gaauuuuaca aauaccuaaa agggauucua     1140

cuaaaaauaa auggaagugg ugacuuuuua gacaaaaucg auugcgaaga cuuccuacgc     1200

aaacaacgaa cauuugacaa cgguagcaua ccacaccaaa uucaucuuca agaaaugcau     1260

gcaauauuac gacgccaagg ggaacauuau ccuuuucuaa aagaaaacca agauaaaaua     1320

gagaaaauau uaacauucag aauacccuau uacguagguc cuuuagcccg aaaaggauca     1380

cgauucgcau gggcagaaua uaaagccgac gaaaagauca ccccuuggaa cuuugaugac     1440

auucucgaua aagaaaaauc agccgaaaaa uucauaacaa gaaugacccu uaacgaucuc     1500

uaccuaccag aagaaaaagu uuuacccaaa cauagcccac ucuacgaaac uuuuacaguc     1560

uauaaugaau uaacaaaagu caaauauguu aacgaacaag gcgaagccaa auucuuugac     1620

acuaacauga aacaagaaau cuuugaccac guuuuuaaag aaaaccgaaa agucaccaag     1680

gauaaacuau ugaacuauuu aaacaaggaa uuugaagaau uuagaauagu uaaucugacc     1740

ggccuugaca aagaaaauaa agcguucaac aguucauuag gcacauacca ugaccuaaga     1800

aaaaucuuag acaaauccuu uuuagacgau aaagcgaaug agaaaaccau agaagacaua     1860

auacaaacuc uaacacuauu ugaagacaga gaaaugauuc gacaaagacu acaaaaauau     1920

agcgauauau ucacaaaagc acaacuaaag aaacuagaac gaagacacua uacagggugg     1980

ggacgcuuaa gcuauaaauu aaucaauggc auacgcaaua aagaaaacaa aaagacaauc     2040

cuagauuauu uaauagacga uggauacgca aaccgaaauu uuaugcaacu aauuaaugac     2100

gacgcucuau cauucaagga agaaauagcc agagcacaaa uaaucggcga cguagacgau     2160

auagcaaaug uaguacauga ccuaccagga ucaccagcga ucaagaaagg aauccuacaa     2220

ucaguuaaaa ucguggacga gcuaguaaaa gucaugggac acaaucccgc caauauaauu     2280

aucgaaaugg caagggaaaa ccaaacuaca gacaaaggca gaagaaauuc acaacaaaga     2340

uuaaaguuau uacaagacuc ucucaaaaac uuagauaauc caguuaauau caaaaacguu     2400

gaaaaccaac aacuccaaaa ugacagacuc uucuuguacu acauacagaa uggaaaagac     2460

auguauacag gugaaacucu agauaucaau aaucuaucac aauacgacau agaccauauu     2520

auuccacaag ccuuuauaaa agauaauucu cuagacaaua gaguacuuac uagaucugau     2580

aaaaacagag guaaaucgga ugauguucca ucaauagaag uaguacacga aaugaaaucc     2640

uuuuggagca aauuacuaag cguuaaauua auuacgcaaa gaaaauucga uaaucucaca     2700

aaagcugaaa gaggcggccu gacagaggaa gacaaagcug gauucauaaa aagacagcua     2760

guugaaacca gacaaauuac gaaacacguu gcacaaaucc uagacgaaag auuuaauacu     2820

gaguuugacg gagcccaacg acgaauaaga aacgucaaga uaaucacccu uaaaucaaac     2880

cuugucucaa auuuccgcaa agaguuugag uuauacaaag uacgagaaau aaacgacuau     2940

caccacgccc augaugccua ccucaaugcg guaguaggaa acgcacuauu acuuaaauac     3000

ccacaauuag aaccagaguu cguauaugga gaauauccca aauauaacag cuauagaagu     3060

agaaaaucag ccacagaaaa auuucuuuuc uacucaaaua uacucagguu cuuuaagaaa     3120

gaagacauac agacaaacga agacggcgaa auagcaugga auaaagagaa gcauaucaaa     3180

auucuuagaa aagucuuauc auauccgcaa guuaacauug ucaagaaaac ggaggaacaa     3240

acagggggcu ucucaaagga aucgauucuc ccuaaaggcg aauccgacaa acuuauccca     3300

agaaaaacca aaaauuccua cuggaaccca aagaaauacg gcggauucga cucucccgua     3360

guggcauauu caauccuagu auucgcagau guagaaaaag gaaaaagcaa gaaacucaga     3420

aaaguucagg acaugguugg cauaacaaua auggaaaaga aacgauucga gaaaaacccc     3480

gucgauuucc uagaacaaag aggauacaga aacguuagac uagagaaaau aauaaaacua     3540

ccaaaauauu cacuauuuga acuagaaaac aaaagacgaa gacuacucgc aagugcacga     3600

gaauuacaaa aagguaacga acuggugaua ccacaacguu ucacaacacu auuauaccac     3660

ucauaccaaa uagagaaaaa cuacgaacca gaacacagag aauacguaga aaaacauaaa     3720

gacgaguuca aagaacucuu agaauacaua aguguauuuu cuagaaaaua uguucucgcc     3780

gacaauaacu uaacaaaaau agaaauguua uuuucaaaga auaaagaugc ugaaguaagu     3840

agccuugcaa aaucauucau aucgcugcuc acauuuacgg cuuucggagc accagcggcg     3900

uucaacuucu uuggugaaaa caucgaccgu aaaagauaca ccuccgucac agaaugccua     3960

aaugccacau uaauacauca aucaauaaca ggguuauacg aaacccgaau ugaccuuagc     4020

aaacucggag aagauuga                                                   4038


<210>  5326
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5326
augaaucaaa ccaaaaacua ugacauaggc cuagauauug gcguaggaag cguuggcugg       60

acaauaaccg acucccaauc ucaaaaagua cucaaaguaa aggggaaaca agguauuggc      120

guacaccugu ucaacgaagg ccaaacagca gaagaacgac gucaauuuag aacaaacagg      180

agaaggcuca agagaagaaa auggcggcuc cgacuacuuc gagaacuauu cgaugaaccc      240

auauccucaa ucgacccaga auuuuucgcu cgucuuaaau acuuuaacga aucaagcaaa      300

gaccccaaaa auaaggguua ucagccacag cuacacgacg acuuaaaaga uguggacuuu      360

uacagacaau aucccacaau auaucaccua cgacacgcau uaaugaccca aaaagaaaaa      420

uucgauauaa gagacauaua ucuagcaaua caccacaucg ucaaauaucg cggccacuuc      480

uuaaugaacg gacauagcag uaguuuuucu guugcccaaa cagauuggaa agcggaccuc      540

acuaaacuau uugaacuaau ccaagaaacc agaacuaacu uagacuuuuc guucgaccua      600

acagaaauag acaccauaaa aucccuacua uuugacaaga accuaucgag aaaugacaga      660

cagaaagaaa uaguaggacu gaucaacaau aauucccagc uagacaagaa agacaaagca      720

cugacuaaac agcuaguaga aucagucguc aaaggaauug uuggaaugaa aacuagaacu      780

gacauacucc uaggcuacga aguugauaac aaagaacaau caaaggcuug gaacuucuca      840

aucgaugaac uagacgacca aauggcaacu auugacccag cccuuagcca aaauucacaa      900

gcacaugaua uaaucacauu gauccaaaaa cuauauauga caguacaauu agcgcaaaua      960

guacccgaag gccaaucugu uucagaaaug augaucaaua gauacaaccu auacgccgaa     1020

caacuacagc aacucaaaga uuacaagaaa auaaacuuag aaacaggccu auuacuaaga     1080

gaagcauacg acgaauugau gaccaaagcc uugacugcug cuccuggaaa aacgauacac     1140

gauacguuuu auaacaaagu caagaaaauc cucaaagauc aagauaacaa acaagcucua     1200

ucaauaaaac aagccauaga agacucuaau uaucuaaucc cccuccgaau uaaaaguaau     1260

ggcgucauuc cauaucaauu gcaccaaaga gaauuagacg caaucauaga aaaccaaaaa     1320

gaauuuuauc cauuucuugc aacccccaac ccuguagaug gacaucaaga agccuuaccc     1380

uacaaauuag augaauuaau cggcuuuaaa guacccuauu acguaggacc aaugguagac     1440

ccugaaguag acuccgacca aaauuucgcu uggaugguac guaaagagaa aggcgaaaua     1500

acucccugga auuuucauaa caaaguagac auaacacaau ccgcagacaa uuuuauaaaa     1560

cgaaugaaaa cgacagauac auauuuaauu ggcaaagacg uccuaccuca aaauucacuu     1620

uuauaucaaa gauauaccgu auuaaaugaa uuaaauggca uaaaagucgc aaauaagaaa     1680

cuaaccauag aacaaaaaca acgacuuuac gaccaacucu uuaaaaagaa gaaaacuguc     1740

aagaaacaag auaucuuaaa caaccucguc gcagcaggag aaauacacga agaugacaau     1800

uuaacaggac uguccaacgg uacaacauuu aacaacaauc uuggauccua uauagacuac     1860

ugcaaaauua ucggacaaga uaaacuagaa gaugcaagua aacaagcaga uauugaaaga     1920

auaauugaau gguccacgac auuugaagac gcaaauauau uucugacaaa acuagcggaa     1980

aucaacuggu uaacagaaga ucagcgccaa aaacuaucgc aaauaagaua ccgcggaugg     2040

ggccaauucu cuaaaacccu uuuaacagac aucaaagaua acaaagacaa uagcaucauu     2100

gacaaccuaa ugucaacuaa gaaaguauuc augcaaauua uaaacgacac aacaauaaaa     2160

cagaaaauug augacuauaa ccaauaucac cuaaaucacg cagaccuaaa agauacaauc     2220

gcuaacuugu uuaccucgcc acaaaauaaa agagcaauaa accaaacacu auuagucauu     2280

gaagacauaa ucaaugcagu cggacacaaa ccaaaucgua ucuucauaga aucagcuaaa     2340

gacaauagaa acgccaaaaa ucaacuaaca caaucaagac uaaaacgcau aguaaaacaa     2400

uucgaagaca auaaacagca cguugaucaa ucaguauaug aggagcuuaa acaacuagac     2460

gacggaucac augcauccau cgacgaccga uuauugcucu acuuucuaca aaacggcaaa     2520

gauauguaua caggacagcc cauugacauu agucaacuau caacaaguca auaccacauu     2580

gaucauauac uaccccaagc aaaaguaaug gauaaugcac uagacaauag aguuuuggua     2640

ucuagcaaag caaacaccga aaaaucagac agauucuuaa gcgaaauacc ucaauuccaa     2700

caacguucuc uaugggcaga acuugaaaga augggcuucc uaacgaaagc aaagaaagaa     2760

cgacuucuaa ugaccucaga ugaauuuaaa caaaacucag uccgauucau cgccagacaa     2820

cuuguagaaa cacgucaaau aauuaagcuc gucaaccaac uaauuauaca agaacacccc     2880

gagguaaagc uaguaaacau caaagccgac cugacaaacg ccuuuagaaa gaaauuuaau     2940

uucgugaaaa acagaaacgc gaacgacuau caucacgccu uugacgccua ccucacaagu     3000

uuaauaggau cacaccuauu gaaaucgcau cccaaauggg aaccacuauu cguuuauagc     3060

gauuaugaua aaauaaaauu cgauaacgcc ccacaaggag uaaauauaau gaaagaguuc     3120

gaacaaucaa aacuugcgga cgaaaaugga gaaauaaucu ggaacaaaga ugaaaugaug     3180

gcauacuugg aacgacuaua uaacucacgc caaauacuua uaacaaagaa agucuacgca     3240

cgacauggca accuauucaa acaaacuauc uuuaaagcug gcgaaaguga caaccucaua     3300

ccagucaaaa auaaucguuc cgacauagcc auauacgggg guuacaaauc aaaacaaguu     3360

uccuacuaug cucucauauc acuacauaac aagaaaagac agaaacgccu aguagcagua     3420

uauuugauag acauacaaaa auuacaaaac uuaaacgaau cagacuauuu caaaacacug     3480

cauacuauca uaaaagaacg acacaacauc aaaccagaau uugacauacu agcagauaaa     3540

auauaccaaa accaacuaau aauagauaau aaaaacggcu ccaacagacc auuccgaauc     3600

gaaggagacu ucaaaaauga ucgacaacug uauuugcccc uucaauacca aagauggcua     3660

gccucaaaag aaccaacuga cgaaaaacua aauagaauau ucgaucacau cugcgaacaa     3720

cucgccgauu auuuuccauu auuugccgga aaugcaugcg acaaacuucu cgacaauaga     3780

gauaaauuuu accaacuauc aauaaaaagc gaguucaacg acaaaggcaa acuaauagca     3840

gaaggaaaac uaucaguaau agauaaauua cucguagcaa ugcacgcaga cucgcgaaau     3900

aguaaucuag cagugauugg acuaaguagu ucauggggca gauucaaacu ucacggagga     3960

aaugaagcua uaggcacauc ugugcaauua auacaccaaa guccaaccgg cuuauucaaa     4020

cgaauacaac gauucaacug a                                               4041


<210>  5327
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5327
auguuaucag cagggguaau guuaggugau gucucagcac ucaagucaag uagaaauuca       60

cuguuucaaa aaucuaagaa auuaauagaa aauucaucau cugaccucac uaaagucgau      120

uauagcaaaa ugcgagaaau acuauuauca auucguaaca uagccauaag auucuucaau      180

aaugaagauu uuaaagaaag cgauuauuau aaagaguucc caaccauaug gcauuugagg      240

aaaaaccuaa uggaaucuga uaaaaaggcu gacauaagau uaguauaccu agcacugcau      300

aauauaguca aauaccgagg aaacuuuuua caggaaaaua auaaagaccu aagagcagcu      360

aacgcaaacg cuggugcagc agcaauagac uuaucuaaug cacuagaaga auacuucuca      420

cugcaugaag augaaucugc auugaccugu guacccaaug uagcagacuu acaaaugacc      480

uuagaacaac caggacuaag aaacggagaa cgauuagaua gaauagucgc agcacuaaau      540

augccagaca gagcuagagc aaaauuauuc gcuaaggcuu guauagguca aaaaguugaa      600

uuuagcaaau uggcccuugg aauggagaaa caagaaggca cgaaugcaua cauaugggau      660

gacgauaaau acgacgaauu aguagcucua ugcccagaag acuucuuccc aguacucgaa      720

acacuuaaaa acgcuuacag ugccuauguu uuaagcgacu uauuagcagg aaaacuaucg      780

auaucugcuu cguuaauaga uucuuacgag caacaccaaa augacuugaa aaucguaaaa      840

gagcuaguua gagaauacuu aggacaaaag gaauacggac guuuauucag aggaccgaaa      900

uuugcaaacg gagacuacaa uuucaauaaa gucgcaaagc caucauacac aagcuacaua      960

cuaggcgaau cacugugcaa caaacaggga acaacaagag aagaauuagu caaagaacug     1020

aaacguguau ucgaugauug uagcgcgaua caagcagaua aaagauacca gaaaauaaaa     1080

caacgcuuag aagaugaucc aaaauuccug aucaaacaga aaacuagggc aaacggagcu     1140

auuccuuauc aauuacaccu agaagaaaug caucaaauaa uagaaaaaca aggaaaauau     1200

uauccauuuc ugguagaaca acaagaucuu auagaaaaac uuguuagcuc ucggauaccc     1260

uauuauguag gcccauuaaa cggaggacau gauccaaauc aucccuaucc uaccaaucca     1320

auagacccca aacggaaauu cgccugguca guccgaaaac cagguaugga acacgaaaag     1380

gcuuacccau ggaauguaga ugaggucaua gacuccgaag aaacagcgga acgcuuuauu     1440

agaaggauga caggaacuug uacauaucuc uauggggagc cuguaguacc ccgauguuca     1500

uuauuauacg aagaauuuug cguauuaaau gaauuaaaug gugcaagaug gggagaggga     1560

aauggcgaca cucauagauu ugauaguagc gauagaacag aaauauugga acaacuauuu     1620

aagaaacgaa aaagaguaac acacaaagca auagcuacau ggcuagagau uaaucacggu     1680

guaacaaaug uaaaaauuuc ugguacacaa gcugaaaaug cauuugaaag caaacuuagc     1740

ucauauaacg auuucuguaa uuugcuggga guugacgguc uagaagaugu uugcccauua     1800

acaaaagaug auauagaaca aauaauacua uggguaacug ucuuugagga uaagaaauua     1860

uuauuacaaa aacuucaaag agcauuugga gauaaauugu cagaaaaaca aauagcauca     1920

auaaugcgca aaccuuaugc agggugggga cggcuaucuu caaaauuauu gacagguauu     1980

aaaagagaaa ccccauucgg uagaauaucc auucuagacg uuaugaggga cggcgaccca     2040

cgaccuggcc accauuugga ugcgaugaau uuuauggaaa uacuaagaga aaaagacuuc     2100

ggauuucagg auagaauaga ugaaauaaau aaagagagaa cuaaggaaag ugggcuccuc     2160

cugucaguag aggaucuacc aggaagcccu gcauuacgaa gaagcguuaa ucaggcaaug     2220

cacauauuga aagaaaucgu aucaauugcu ggcaaagaac caagccgcau aguaauagaa     2280

guuacgagag acccagacga agagaaaagg ggacaucgca cugauacaag auauaaacaa     2340

uuaagcgaag cauuagcagc gaucaaaacg gacauaguac aauuugacuc aucacuacua     2400

gcagaacuca aggaaagaaa ggacagucug gauucagaac guuuaaugcu cuauuuucag     2460

caaugcggaa aaucucuaua uagugggaaa ccacuagaca uaaaccgauu aagugaauac     2520

caggucgauc acauuauccc acaauguuac auaaaagacg auaguuuaga caauaaagcc     2580

cuuguauuug caagcgaaaa ucagauaaaa uuggauagcu uguuacuaga caaaucaaua     2640

auuagaucuc aaaaagggag auggcaggaa cuccacaggg caggcuuaau aucaaacaag     2700

aaauuucgaa acuuaaccug cacugaaaua ucagacagaa ugaugaaagg auucauaaac     2760

aggcaguugg uagaaacauc acaaauagua aaauuugugc gacaaaugug ugaacaacgc     2820

uaugaauccu ccgaagugau cucacugaga gccagucuga gccauaaccu aagagacaga     2880

uguggcuuca uaaaauguag agagcugaau gauuaucauc acgcacacga ugccuaccua     2940

gcuugucaaa uugcaagauu caucggaauu agauauccca gauggcagga cggaauauca     3000

agagaauuua uaaauaagua caucaaacaa uuagucaaga aaggaaaaag cucaggcaaa     3060

uucuuugcag gaaaaucugg auuuauaaua gauucauuca ugcaugacgg cuuugaucga     3120

gaaaccggcg aaauauuucg ugauggaugg gacgcaacuu ucgaaauaac gagaaugcga     3180

aggguauuag gauauaaaga uugcuucaua acaagaaugc cagaagaaca gacaggagcc     3240

uacugggaag aaacaauaua cucaccauug gacgcgaaaa acgggaaaaa cuugcaaaua     3300

cccuuaaaac agucaggaaa ggaaggauau uuggauccaa agaaauacgg aggaacaaac     3360

accgcuuuua augcauauuu cuuugcauuu gucgcaaaag augcaagggg aagggacaaa     3420

uauuucuuug aagguguacc gguuuauuua gcaaaacaau ugaacgaaca accacaaaua     3480

uuaguugauu uugcuaauag cauagcugcg acuaagaaau gcagcacagc aaaaauacua     3540

aggucuaaag uuccuuuaag gcaaaaauuc cucuuagaug guacaaguuu cuauuuaggc     3600

ggacgaacug gcaauaaaaa ugugauacua agcggcguag aguuugcaug cgguauugac     3660

auggcaaaaa ugauagauac ugcgauaaaa cacccggagg aauuaucuca aaaagaauua     3720

auaucuauaa uacaaauguu agcagacaca auugaaagaa auucggauag acaagcaagc     3780

cuuauaaauu uuagaagguu ugacgauaaa uuaagaaaua ucgaugcaau cgcugcaucc     3840

agaauaauaa agaaucuacu caauucuaug aaugggguua aacagagcug cgaucuauca     3900

gauuuaggag guagcaaaca aucagguuuu augcaaagua auauagcaac ucuauucgaa     3960

agaauuacau gguguuauuc aaguauuaca ggaauguucg aacaacgagu gaguuucgaa     4020

gaucugaaaa acggaaucug a                                               4041


<210>  5328
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5328
augaaaaaaa gaaacagaga ggaauacuac guaggacugg auauaggaac gucaucagua       60

ggcugggccg uggcaacacc agacuaucgu auaccgaaau uccgcgguca caaaaugugg      120

ggaucaagac uuuuugccga aggaaaacgc gcagcagaac gaagacaauu uaggacaaac      180

agaagaaggc uagcaagaag acgagaucga cuccaaauuc uggaagaauu auuugcugaa      240

gaaauggcua aaguugauaa uacauuuuuc augagguuaa aagaaaguaa auucuucuua      300

gaagacaaaa cagucaaaga gaaacauauc uuauucucaa cugacaaguu cgacgcagau      360

cguuuuuaca aagacaaucc caccauauau cacgucagaa aaagacucau ggaagaggaa      420

ccucaagaua uacgagaauu auuucuugca guucaccaca uauugaagua cagaggccac      480

uuucuguucg cagggaguuu agaacaaaau ggugauagua uugaagaacu cgugagaaca      540

cuauucggua aacaaggccu agccauucuu gacgauacaa uagacucuaa cgaaauggua      600

aauauauuaa aaaauaacga aaaaacaaaa ucagauagaa aaaaggcagu acaggagcua      660

uggaaccccc uuaaagacaa aguuaaaaaa gcucaagcug cugaaauggc cggcguaguu      720

uuagggcuaa acuccgacau cucgaaauug uuugacaauu ccgauuauaa agacuugaaa      780

auaaacuuca guucaggaga cuacgacgag aaaagagacg acuaugcuaa cucuauagga      840

gaugacauug uguugcuaga uaccaugaaa gcacuuuacg acacacuaau acuaucccag      900

auauuacaag gaaagcaauc cauaucagaa gcuaaagugg aagauuauaa cacccauaaa      960

gaacaacuca gggcauuaaa agcaauucuc aaaccacaua aaagguuaua uaaugaaaug     1020

uucagagaaa auaauaaaaa agaccuaucu aauuacguag cuuacauaag cucauacucc     1080

gaaggaaaaa ggguaaauag agaaggguuu uauaaauaug uacaagcagu cuuaaaacaa     1140

guagacgaua gcgacucaaa acaagcgaua uuagaagcga uacgacuaga aaccuuccuc     1200

ccucuacuuc gaguaaagga uaacgccguu auaccacacc aaauucauca agcagagcuu     1260

gaagcgauac ucgagacagc uagaaagaaa uauaaauucc ucaacgaugu uucagaaggu     1320

guaagcguca aagauaaaau cauccagcua uuuaccuuua gaauucccua cuacguagga     1380

ccucugaauc cacacagugg agagacagug gcagacagaa aacaagguac cggcugggua     1440

guuagaaaac aaagaggaaa agucuaccca uggaacuuug cugauauggu agacguaaag     1500

gcaucagcug aagcuuuuau aacuaaccua acaaacaaau gcacauacuu aaucggcgaa     1560

gacguauuac caaaacgauc auuacuauac ucugaauuua uguuacuaaa cgaacuaaau     1620

augauaaaau augacggaca ugaacugccu guugaaauca aacgacuaua uauagaacaa     1680

gucuucaaac aagaacacaa aaagcaaaca aaaggaagag uacuagauuu ccuuaaacgg     1740

aauggauuaa ucacaggauc cgcaaccgca gucaucaccg gaauggacga uucaauaaaa     1800

ggcgaucucc agucguacag agauauggua cgaauauuug guaccggguu cgaccacuca     1860

auggccgaaa gaauaauaag guggguaaca cuguucggug aagcaaaaga cauauuagaa     1920

aauaagauaa gagaaguaua uggcaauagc auuuccgauu cacaaaugag agcuauuaaa     1980

aaacucaaau acgccgacug gggaagacua agucaagcau ucuuuauaaa aauaauagga     2040

ugcgacaaau caggaguaga acgaaacaua auaaccuguc ugagagaaag uucccagaau     2100

uuaauggaaa uauuaucaga ccagggaacc uauauaaaac aaauaaaagc auggaaucau     2160

cagcauacaa caaacaugau ggauacucua gacuaucaau uagucgauga cuuguacguu     2220

gccccauccg uuaaaagguc aauuuggcag ucacuaugua uaguugaaga auuaguaaaa     2280

auaauggguc aagccccaaa aaaaauauuc guagaaguaa cuagaacaaa ccaagcagaa     2340

aaaaccaaga aagacagucg caaaaaaaga cuaguagaac uauacaccgc aaucggcaaa     2400

gaaggagcag cuuggaaaaa agaaauagaa aagcaccaag caggugauuu ucgaagaaaa     2460

aaauuauacc uauauuacac ucaaauggga cgauguaugu auucacauga accaauugcu     2520

auggacgcac uuuucacguc agaauacgac auagaccaca uauacccaca aagccucacu     2580

aaagacgauu caugggacaa ccuagugcua guaaaaaguc agcucaauaa agaaaaaucu     2640

gaccaauacc cccucaggcc ggcaauacga gaaaagaaca aagcauauug gcaauaucuc     2700

ugcagggcag guuuuauaag ugagaaaaaa uaugaaagau uaaugaggac gacagaacug     2760

acagcugaag aacuaagugg uuucaucgca cgacaguuag uugaaacaag ucaagcuacc     2820

aaagcaguaa uuggacugcu ugaaagaauc uacaaacaua cggacauaug cuucgugaaa     2880

gcugaaacag uaucccaauu ucgacaucaa cacaacuuua uaaaaauaag agaacuaaau     2940

aacuaccauc augcuaaaga ugcguaucuc aacauagucg uaggcaacgu auaccacgaa     3000

aaauuuacaa aaaauccaag agaauuuauu gcaagaucaa ccgaacgaag accauacaau     3060

cucaacagaa uguacgacuu cgacuuacuc aaacaaggca gagugauaug gcagguccca     3120

aaaucccugg agaccguaca agccaugaug aaaucaaaug acguuagaau aacaaaaaag     3180

cuuuuagucc agaagggcga acucuacgau gcaacaauua caaaagcaca uaaaacuaaa     3240

gaaggaaugu acuucccacu gaaaggggcu gaugcaagac uacgcgaugu ucauagauac     3300

ggaggauacg agaaaauaaa gauagcauau uacucaguau ucagcugcug ugagaaagcc     3360

agcgauacga uacauacacu cauuaucccc auaccaguau augcagccgg auuacuacag     3420

acagaagcag auuuaacagc cuacgcuugg cagacgaagc aaaaaacaaa accaauaaaa     3480

gauaucuauu cuaugauaac accaauauac aagaaacuuu guauaaacac aguuguuaaa     3540

auugacggau uuaaauauuu ucuaggugga agaacagaag acuauauaug cuuauaccca     3600

gcuauuccag ucguacuuga ugaacaagcg gcagcauauc uaaaagcagu aagcaaagua     3660

gguagagcug acgauguaga gaacaccgcu caagcaauag accuaauaac ugcugaagcg     3720

aaugaagcau ucuacgacgu acuacuaaaa aaaauggaag caaaaauuuu uguuaaagcc     3780

uaccaggaaa gaguagaaaa auuagcaagc gaaacggcuc gagaacgguu ucggaagcuc     3840

uccauaggag cgcagugcag cugccucuua gaaauacuaa acuugcuagc aaauaacaaa     3900

acaacuucca acuuaaaaaa auggcuuggg guuacuauga gcagaagaaa acaaccaaug     3960

gacuuaacaa aacucgaaga guguauagua uaugaaacau ccauuacagg auuauacgag     4020

aaagcggugu cauuacuaug a                                               4041


<210>  5329
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5329
auggucgaac acuacgcaau agguuuugac auaggaacug gaaguaccgg cuuugcgggc       60

guagauccca auggaaaauu gguacguaug cgagguaaaa acguauaugg aguauauagg      120

uucaaaggag gcgaagccgc ggcagaccga agaggauuua ggacaaccag aagacgcauu      180

aaaagacgaa aauggcgacu aagauuucua aaaucuuucu uugaaccaua uauugcaccu      240

aucgaccccg aguuuuuccu acgucaaaaa uauucggcug acucaccacu agacccgaaa      300

ggaauacggg ccgauaaagg acuguuauca gacaugucag aucaagaguu ccaugcuaaa      360

uauccaacaa uuuaccaucu aagauaccaa cuaaugcacg agaaaagaca auucgacgua      420

cgcgaaauuu aucuagcaau gcaccacauc auaaaauaca gaggacauuu ccuuauagaa      480

gacggcgcag auaauuucga ugcaaaacaa auugacuucu uugaccaguu ccaaagaaug      540

gcagcgguua cagcaggacu cccagaacua gaaacuuuag uaguuccaga cgaaacucag      600

ucagaugcuu ucaaacgugu acucuuugac gaaagccaaa cuaaauccga uagacaaaaa      660

guacuagcca gccaacuauu agacauacaa gacaaccaau caaaagcaau caaagauuuu      720

auagcagcac uaacuaaagc ggucgucggu augaaagcaa aucuugccac ccucacuucc      780

acagccauag aaaguacaga uaaaaacgau uggacaaaac cgcuaggaca auuagacgau      840

uuccuacuca aacaugaaga agacuuagac gaagacggau acgcuuuaau ugaugcugca      900

aaucaaaugu acagugcugc aguacuugca gcacucgucc cugaagggau gggauuuuca      960

gaagccauga uggcuaggua cgcccagcac aagaaacaau uagcacuaua caaacaagua     1020

auagcgaguc uaccugacaa aacucaaaga cacgagcuaa aagacgcaua ugcugauuau     1080

auagagcacg cagcucaaaa uccacgcgau gcuuucuaua aaagaguaac uaaagucuug     1140

gaagguguag acucagcaga cgcccgcaca guaaugacag caauuagcca cgacgaauuu     1200

cuucuaaaac aaagaacacu aucaaauggg aaaaucccau accaaaugca acaacacgaa     1260

uuugaugcua uuauagaaaa ucaaaaagca uacuauccau ggcuggcaac acccaaucca     1320

gucgaagcag acgcaaaacg auugcccuac uaccuugacg aauuaauagg auuuagagua     1380

ccauauuaug ucggcccgau gguuacugaa aaacaaucac aauuugcuug gaugaucaga     1440

aaagcagacg gagaauuaac accauggaac uucuucgaug uaguagacag agagaaaacc     1500

gcuaauagau ucauccagag aaugcaaaca acagauaaau acauucuagg cgaagaugug     1560

cuaccaaaac acagcauucu auaccaacaa uucgaagucu uaaaugaacu aaauaacauu     1620

cgaguggaaa aacacccauu aacaaccgaa caaaaacaau uuaucuauca aagauuauuc     1680

guugaccaac agagaaaaac aauaaccaag aaaaaucuac aagauacccu uguagcuaug     1740

ggugaguucg acaaagcgcc ugauauaggc ggacuagcua aagaagaugu aaugaugucu     1800

agcuuagcua cauuugcaga cuauucuaaa auacuucccg aagccauuuc agacccuacu     1860

agacaaaacg acauagaaga uaucaucgcc uggucaacaa uauuugaaga uacacagaua     1920

uucuuugaua aacugaaaac cguugacugg cuaacaacuc aacaacguaa aagauuagcc     1980

ucaauuagau acacuggaug gggaaaacuu ucaaaacgcc uauugcaaca aauuccuaca     2040

ucuaaacaca caaauguaau ggcagcauua ugggcaacau cagauaauuu uaugcagauu     2100

cuucaagaug aacaauuuca agaagggcug uacgacgcaa acagauuacc agaagaaaca     2160

cucacucccg acgaacaggu cgagaaaaua cucgaugaag ccuaccuaag cccagcagac     2220

aagaaaggag cacgcaaagu gauaaaaguc augaacgaca uuguaagggc uaaucaugga     2280

guacuccccg aacaaguuuu ucucgaauca gcagacgauc cacagaaaaa uaaaaggaga     2340

agcguuaguc gaucaaaaca auuagaagaa aaauaucguc aagcagccca aucauucauu     2400

gacgaagccg ugaaagaaga auuaaacgca caaauaguca auucagcuga auuuaacgac     2460

agauuaguau uauacuuuac ucaaaauggu agagacaugu auacaggaaa aagacucgac     2520

auagaaaauc ucgguucuua ccaaauugau cacauauuac cucaauccuu aguaaaagau     2580

gacucccuag auaauagagc gcuaacagca cgugauauca acgcagaaau gucuaaccaa     2640

uuugugucug aaagauuugg auuaggccca agagcauuuu ggcaacaacu uaaaaauaau     2700

ggacuaauau cuggcagaaa auuucgaaau cuaguuaugc aacgcgauga aauugacaaa     2760

uacgcacccg guuucauuaa ucgccaacug gucgaaacaa ggcaaauuau aaaaacaguc     2820

gaacaaauua uucaucgcuu auggccugau auccaaaucg ugacaauaaa agcaaaucuu     2880

acacaucagg uacguucuga auuaggguuc ccuaaaguaa gggagcuaaa cgacuaccac     2940

caugcguucg acgcccucuu agccgcaaga auuggucaau acuugcuaga gcacuacgca     3000

ggucuuaaac cauucuucac uuacggggaa uuuaaaagag gcgacacuaa gaaauacgga     3060

acaacaaacu ucuugagaag auuaacaaaa gacaauaaag acaagaaagg agaacuuuug     3120

cccgaaguaa gcgacuggau agacagcaua gauaguauuu acaauuacaa gaaaauacca     3180

guaguauggg aaaacacaac aaacaugagu gcacuguuug accaaacuau auauccgaga     3240

ccagaccgcg acacaacaaa aagaaaacuu auaccuacaa aauuagacaa accuacugac     3300

auauauggcg gauacaguuc ugccaaaaca gcauauaugg caguaaugcg aguacaaacu     3360

aagaaaggaa cugaauaccc aguauuuaau auccccguuu cuauagcgag ucaacuaaau     3420

cgcauaacag accaaacaau gcucaaacaa caagugaaac aguucauaau caaugcggaa     3480

caaucugcua guaaaaagaa acuuuacgaa ucaggagacg uuauacuauc aagaguagca     3540

uugggacaaa gauucacaga uaaaaacuac ggagaaguau ucuuaauauc caauauggaa     3600

guacagaauc cccagucuuu augguuaggu cgcgcuucac aacaacgucu aacacgagaa     3660

cccgaaggua acgaaaaacc agaaaccucu cuugcagaca ucuaucagca aauacuaaaa     3720

caagucgaua gguauuuucu ugcauucaac auacgacaac aaagacaaaa acuauuaggu     3780

acucuugauc aauuuacauc gcuuccagua ggaagaguua acgagcaaac cagaaccgau     3840

caaaaccaaa gaucuaucau cgcacgagua augauaguac uaggcaaagg uucaagggaa     3900

gagaaauuuc cagaauuugg acuuucagcu uuaggucgau uagucagaac aggcggaauu     3960

acacuguccg aagacgcaca aaucaucuac caaucaccaa caggcuuauu ugaacgacgc     4020

guagccuuga aagaucugug a                                               4041


<210>  5330
<211>  4041
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5330
auggcaaaaa uauuaggacu ggaccuagga accaauucaa uaggaugggc gguuguugac       60

auagaaaaca auaacgaauu uacacuuaua gaaaaaggag ucagaauauu ucaagaagga      120

guaaaaauag aaaaaggaau agaaggaagc aaagcagcag aacgaacaac uuauagaucc      180

gcacgaagaa uaaaauaucg aagaaaacug cgaaaaauac aacuauuagg cgucuuaucc      240

gaaaacggau acuguccguu cuuguccaaa gacgaauuag acucauggag auacaagaaa      300

guauauccca caaauaaauc uuuucuaaau uggcagaaaa ccaaugaaau acaacuagaa      360

aacccguauu uuuacagagc ccuuuuagua gagaagaaac uaaaccugaa uauagaagaa      420

gaccguuuua aaauagggag agccuucuau caccuagcuc aaagaagagg uuucuuaagc      480

aauaggaugg aaggaacuaa agaaaacgau ggaacuguaa uaaaaucaau aacagaaauc      540

ucaacagcaa aaggagacaa aacacuaggu caauauuucu acgaacgaua caagaaagga      600

gagaaaauaa gagauacaua cacacacagg gaagaacacu aucuagaaga guucguccgu      660

auuugugaau uucaacaacu ggacaacaaa uucgcggaaa aaguaagaaa agcuauauuu      720

uaucaaagac cccugaaauc ucaaaaagga cuaauaggca aaugcguauu cgaaccuaag      780

aaaucucgcu gcaguguaag ucgaccagaa uuugaagaau aucgaaugcu augcuuccuu      840

aauaacauaa aaauuaaaac accaucagac gaaaauuuaa gauuuuugac agacaacgaa      900

aaagaaaaac uuaaaccuca auucuuucga aaaucaaaag aucacuuuga cuucgaagac      960

uuaguaaaac aacuagcgcc aaagaaucaa uucaaguuuu acaaaucaag agacauauug     1020

ccagguaacu ggcuauuuaa cuacagcaug aacacuacgg uauccggaug uccaaucucg     1080

gcaagacuga aagaucucuu cggugaaaau uucauggaua uaaaauucga cuauaucaga     1140

gaaaaagaug ggaaaaaguc aguaaucgac auacaugaca uuuggcacgu ccucuucacc     1200

uacgacucug aagaaaaauu aaaagaauuu gcccggaaca gauuaaaccu agacgauaua     1260

aaaauaaaag aguuccaaag gauucaacuc aagaaagaau augcagcguu aucgcuuaaa     1320

gcaauaagaa aaauucuacc auuccuacga gaaggacuca uauauucaca ugcuguauuc     1380

aucgcaaaca uggaaacagc aauucccuca gaaaucugga auaauuauac caacaggaac     1440

auuaucaaaa acgaaauaua caacauaauc ucaaaucaaa cugaagauaa acaaguaguu     1500

gacaucguua acggaauaau uaaaaguagu agaauagaaa auagcguaug guccaacgaa     1560

gcuguagaau uuuucaagaa agaucuaaac aguaaaauua agaauuacua uggaauaaag     1620

acauacaacg acuuuccaga agaaaagaaa aucagaauag aaaccuacgc guucaaucua     1680

uuuaaacaac agaugaugaa aaacuuugga aggggagaau uugcagcggu ucaaagaaua     1740

gacgaacgcc uuaaauuauu uuuaacagac aacuucaaca uagaccccau acuacucaag     1800

aaaauuuacc aucccagcgc cauagaaguc uaccaaccac ccguaaggaa aaacgacggc     1860

aaauuauacu ugggaucucc uauggugagu uccauaagaa acccaauggc caugcgugcc     1920

cuccaucaau uaagaaaauu gauaaaugaa cuuauaagaa aagaauugau cgauucuaau     1980

acaaagguua acauagaaau ggcacgagaa cuaaugaacg cuaaugaaag aaaggcuuac     2040

caaaacuggc aaaacgaccg cgagaaaaua agaaaagaau acgcacugaa aauaaaacaa     2100

caucuagacg ucgacaaaga accauccgac guagacaucc uuaaauacca auuaugggaa     2160

gaacaaaacc acaaaugcau auauacagga aaagaaauca acauaagcga cuuccuuggg     2220

agcaacccua aauucgacau agaacauaca auaccucgcu caaucaguuu ugauaacucu     2280

caauccaaca aaacauuaug ugaaaacuac uucaacagau caguaaagaa aaaucgaaua     2340

ccagcugcac uaccaaacca uggcgaaaua auugaaagac uagaaaacug gaaacugaaa     2400

uaugaagaau uagaaaaaca aauacaauac gcaguacgac aagcaaaagg agcaggcgau     2460

aaggauucua aagauagagc cauacagaaa agacauaaac uuaaauucga auaugauuac     2520

uggaaaaaca aauacgacag auuuguuaug gaagacgucc cagaaggauu caaaaacuca     2580

caauugguug auacugggau aauuacaaaa uacucaagga uguaccuaaa uacguuauuu     2640

gacaaaguau acacaguaaa agguaacaca guagccgacu uccggaaguu guggggcauu     2700

caagacgaau augaaaagaa agcucgugua aaccauucac aucacugugu ugaugccaua     2760

acaauugccu gcauaacgaa aaguaauuau gaaaauuuag ccaaauuuua ccaugauugg     2820

gaagaauuac acguaaaggg aauugaccaa cuaccaccaa ugaacaaacc auggaaaacc     2880

uuuacagaag acaucaaagc aauacaauac gagauccuag uaagccauua uacacccgac     2940

aaccuuccaa aacaaucaaa gaaaauacuu cguaagaaag ggagaauaca gaaaaaugag     3000

aacggaaacc caauauaccu caaaggagac acugucagag gaucacuuca ccaacaaaca     3060

uuuuauggcg ccaucgagaa aaccgaagug aauaagaaag gagaacccga aaaaguaauc     3120

aaauacguug uucgaaagag ccucgacgua cucgacgacu ccucaguuaa aaacauaguu     3180

gacgauaucg uaaaagaaau aguccaaaac gccaggauaa aagaaaaaga uuuuauaaaa     3240

gagcuugaaa uacaaaaacg aaaacuagca aaagcagaag aaaacaaggu aguccaaaua     3300

caagccgaaa uagacaaacu aaacaaacaa gucgcggauc uguauacuau gccuaauaaa     3360

aacggaaauc caauaccuau caagaaagua aggauauucc agacuucagu aacaaaucca     3420

auccaucuca aacaacaaag agacaaaagc cagaaaacuu cgaaaccaca uaaagaguuc     3480

uacuacgcug caaacgacuc aaacuacaug auggcaaucu acgaaggcaa agacgaaaaa     3540

ggaaaaguua aaagagacuu caaacuauug aacaaucuag aagcaggaga guucuuuaaa     3600

uacagcguac acaaagaucu aaaagagaaa aauuuagaaa auauaagaaa uguuauacca     3660

gacaagguaa gcgcaggaaa aaucgaaauc ccauuaaaag ccauaauaaa aacaggcaca     3720

augguaauac ucugggaaaa uagcccagau gaaguguggg aucucaccau auccgaagua     3780

gauaaaagau uauacaaagu aguaggaaug ucagucaaca ggaucaaauc agauguaaaa     3840

acauucgagu ucggaaacau cguacuacgu cauaacaaag aagcaagucc cgcuacuuug     3900

cuuaaaauac aggacggaga guucaaauca gacgaaaacu acauagcaca aagaaaacua     3960

ucucacaacc aauucaacgc ucuagucgaa ggaguugauu uuucguuaag uguacuaggu     4020

gaaaucaaac gacucaaaug a                                               4041


<210>  5331
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5331
augggagaag augggaagcc aguacgugua aaaggaaaaa cagcuauagg ugcacgccug       60

uuucaagaag gacaugucgc agccgauagg cgaauguuca gaacaaccag aaggagacua      120

aacagaagaa aauggcgacu aaaauuauua gaugagauau uugacccaua cauaacccca      180

guagacagua cauuuuucgc cagacuuaag caaagcaacu uaucaccgaa agacucuagg      240

aaagaauuca aggggucaau guuauuucca gaucuaacag acaaacaaua ccaugaagac      300

uacccuacua uauaucaucu acguagagcu uuaaugacag aagacaagaa auuugauauc      360

agaauggugu acuuggcaau ucaccauauu gucaaauacc gaggaaauuu ucuaaacagu      420

gcaacgguag auaccuuuaa agcgucgaaa guagauuuca aaaaauccuu uaaccaacug      480

aacgagcuuu auucagccau caacccugaa gacgcauucc aaguagauau gaauaaaagu      540

gaugaaacug gucgucuauu cuuagacucg ucaauccgga aguuugacaa acaaaaacaa      600

auaucaaagu uaauaccagu agugguagaa gauaaagaag caaaaaagcu aaacggcaaa      660

aucgcacuag aaauaacaaa agcaauucua gguuacaaau cgaaacuaaa uguuguaaca      720

caaacagaac cagaagaccc aaaaaaaugg gccguaaaau uugaugacga agauaucgac      780

ucgaaacuua augauauucu cccagaaaug gacgaaaacc aacaauccau agucgaaauu      840

auacaagauc uauauucaca agugacucug aaucaaaucg uccccaacgg aaugucauua      900

ucugacagua ugauaaaaaa auauaacgac caccacgaac aucugaaacu cuacaaaaaa      960

cuuauagaca auauaaguga uaccaaaaaa cgcucugcgc ucaaaacagc guacgauaaa     1020

uacguaggug augauggaaa gacaauagaa caagaugaau uuuacaguaa cgucaaaaaa     1080

aauuuagaug acagugaucu aaguaaacaa auagcagauu uaauagaagc cgagaaauuu     1140

augcccaaac agcgcacaag ccaaaacgga guaauaccgc aucaacugca ccaaagggaa     1200

uuagaugaaa uaauagaaca ccaaucaaaa uauuaucccu gguucgcgga agcaaaucca     1260

aacaaacaug accuccaccu agcgaaauac aaaauagaag aauugguugc auuccgcauc     1320

ccauacuaug uuggcccaau gauaacucca gaagaccaag aaaagucuuc acaaacuaua     1380

uuugccugga ugaagagaaa ggaaccaggg caaauuaccc cauggaauuu cgacgaaaaa     1440

guagacagaa uggaaagugc aaaucgguuu auaaaacgua ugacaacaaa agacacauac     1500

cuaauuggcg aggacguacu accagaucaa ucacuacuuu augaaaaauu uaagguacua     1560

aacgaacuaa auaugguaag aguaaacgac cacccuuuaa aagucucuga aaaacaagcg     1620

auuuauagag auuuauucga aaaucaaaaa agaauaucag uuaaaaaucu ucaaaauuau     1680

guaaaaagua aaagcggauu accaacggga cccaagauau cgggacucag cgacccggac     1740

cacuuuaauu cuagccucgg cacuuauaau gauuucaaaa aaauguuugg agacaaagug     1800

gaugacccag aucuucaaga cgacuucgaa aaaaugauag aauggucgac aauauucgaa     1860

gacaaaaaaa uacucagagc uaaacuagac gaaguagcau ggcuaagcga uacgcaaaaa     1920

aacgaauuca aagaaauuag auaucaagga uggggcagac uaaguaaaaa auuacuuaca     1980

ggaauagucg auggaaaugg acagcgaauc auagacgaac uauggaacac aaauaaaaau     2040

uucaugcaaa uccaaucgga ugauucuuuu gcaaaacgga uacacgaagc gaacgcuaau     2100

caaaugaagg caacagacgu cgaagacguc cuagccgaug cuuauaccuc cccacaaaau     2160

aaaaaagcaa uaagacaagu aguaaaagua gucgaugaua uucaaaaagc uaugggcgga     2220

guugcuccca aauucauaag uauagaauuc acacgaagcg aagaucgaaa cccaagaaga     2280

accauaucga gacaaagaca acuagaaaau acccucaagg acacagcaca agcacuuguc     2340

aaaucaauaa acccggauau acugagugaa uuagauaaug cauccaaauc aaaaaaaggg     2400

uuaacggaca gauuauacuu auacuuuaca caauuaggaa aagauaugua uaccggaaaa     2460

cccauaaaua ucgacgagau caguaacuau gauaucgacc auauauuccc ccaagcauuu     2520

auaaaagacg acucacuaaa uaauagggua cuagucagca aagcaggaaa uaacgguaaa     2580

ucggccaacg uaccacuaaa gcuauucgga gcuaaaaugg gugguuauug gaaacaacua     2640

gcagacgcag gucuaauagg acaaagaaaa uuaaaaaauu uacaaacaaa cccagacaca     2700

auaaguaaau uugcuaugca cggauucaua agacgccaac uaguugaaac cagccaagua     2760

aucaaauuag uggcaaacau acuaggugac aaauacagaa acgacgacac aaaaauaaua     2820

gaaauaacag cuagaaugaa ccaucaaaug cgugaugaau ucggcuucau aaaaaacaga     2880

gaaauuaaug acuaccacca ugcuuucgau gcauaccuaa cugccuuuuu aggccauuac     2940

uuauaucacc gauacauuaa acuccgacca uacuucguau auggcgauuu caaaaaauuc     3000

agagaagaca aaguaacuau gagaaauuuc aacuucuuac acgaccuaac ggacgauaaa     3060

caagaacgca uagcagauac ugagacugga gaaguaaucu gggaucgcaa aaggaguauu     3120

cagcaauuaa gagauguaua ccacuacaaa uuuaugcuaa uaucacaaga agucuuuuca     3180

cucaacaaag aaauguucaa acaaaaacua uuccccgcaa cacuagcuaa aaagagcaaa     3240

uuaauccaaa uaaaaaagga uaaacccguu gauauuuacg gaggauacuc caauaauacc     3300

gacgcauacc uaaccguugu uaaacuuaaa ggaucaaaag aagauaaaua caaaauugua     3360

ggaguaccaa ugagggcacu agauaacuua aaacacgcug agaaagaguc accccaauca     3420

uauacagcug aacuccguaa agcauuagaa ccacaauuca caaagauaaa aaaaaacaga     3480

aaaacaggug aagugucaaa aguuguuacg aacuuugaca uuguauuagg acgacucuuc     3540

uaccggcaaa ugguuauuga cggaagcgaa aaaucacuaa uagccucuag cguguaccaa     3600

uacaaugcca agcaacuagu ccuuucagau caagcaauca gaacaauauc agaucaggaa     3660

ggaauagacg aauuugaauu aaguucgcaa uacgacaaag uuuacgacga aauaguacag     3720

aaaguugaaa aacuacuucc cauauuucaa caccgacauu uucaggagcg cuuaaauaac     3780

ggcaaaucuc uauucguaaa uuugccagua cacgauguuu acgaaggaaa uaagcucgaa     3840

caagucggga aaaaacauau aauuaauuca auacuaggag gaauccacgc caaugccgac     3900

cgaccagaua uugacuuauu uggcauaaaa aauuuaggac aguuuucauc uaccagcgga     3960

auaaaccuau caucugaugc gaucauaugu uaccaaaguc cgacuggacu auuugaaaga     4020

agaguuguuc uaaaagacuu auga                                            4044


<210>  5332
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5332
augacaaagc gcgaagaacc cuacaaugua ggauuagaua ucggaucaag cucaguaggg       60

ugggccgucg uagacaacaa uuaccaucug cuaaauauaa agaaaaauaa uuugugggga      120

gcaagacuau ucaaagaagc ugaaacagca caaguuaccc guggacaccg cucaaugcga      180

agacgauaua gacgucgaag aaacagauug aacuggcucg acgaacuauu cgcagaugaa      240

uuagcgaaga ucgacccauc cuucuuacua aggaugaaaa acucaugggu caguaaaaaa      300

gaaucuaccc gcaaaagaga cccauacaau cuauucauag augaaaaaua caaugacguc      360

gauuauuuca accaauaccc aacaauuuuc caucucagaa aagaacuaau aacugaagac      420

aaaaaaguag acaucagacu caucuaccua gcaauacaua auauaauaaa guacagaggu      480

aacuuuaccc uagaaaacca aaauuucgau aucucgcaau ugucuucgaa cuucucccaa      540

caaauaucug acuucuucgc ccuauuuuca gacuuuggag ucauaaugcc agaagauuuu      600

gauccugaca agauaucuga cauucuacug aauccaaacc uaucaccguc aggaaaagua      660

ucagaagcaa ucgcaacaau cucaccaaaa accaacguaa aagcaaaaau aaagaucaua      720

cuauuauuau uggucggaaa caauggugac cuuaaaaaac uguuugaccu cgaaaccacu      780

gaaaaaaucg cuguaaaacu uucaucucgg cauauagauu cagaacuccc aauaauacua      840

uccgaacuua acgaacaaca agaaaauauc aucacaauug ccaauucaau auuuggcuca      900

auaauauuaa aagauuuccu uggugacgaa acaucaauau ccgcugcaaa aguuauaucg      960

uucgaagauc auaaacaaga ucuacagaaa uuaaaaacaa uguggagaga aacauccaac     1020

aaagaagcug uaaaagcggg aaaaaaagca uaugaagacu auauaggcca cgaagauagc     1080

gaaacauucu auaaaaaaau caaaaaguuu cuugaaaaag cacaaccugu agaucucgcc     1140

aacaaagcac ucgccgagau agaauuggaa aacuaccucc ccaaacagcg gaaccgaaac     1200

aacaccguca uuccauacca acuaaacgaa aacgaacuaa uagcuauacu ugaucaucaa     1260

gaaaaauauu accccuuuuu aaaggaaaau cgugauaaaa uacucucacu uuuaacauuu     1320

agaauucccu auuacguagg cccguuacag gacucuaaca acaacagauu cucuuggaug     1380

acccgaaaag cuaguggagc uaucagaccc uggaauuucu cagaaaaagu caacguagaa     1440

caaaguagca acgacuucau caaacgaaug aggucaacag auacauauuu aauuggagaa     1500

ccggugcuac caaaaaaauc auuaauauac caguguuacg aaguacucag cgaguuaaau     1560

aacacaagag uaaaagacgg gucuagcaac ccaaaacggc uagacguaac aauaaaacaa     1620

agaauauaca augaaauauu uaaaaaucaa aaaucaguau ccguuaaagu ucuucaaaau     1680

uggcuaauaa aagagucuua cuucaaauca ccagaaaucu caggacuagc ugauaaaaaa     1740

aaauauuuaa gcagucuauc gaccuacaua gacuuuaaaa aaauauuugg acaacgcuuu     1800

gucgacgauc caguaaacuc cccccaauua gaagaacuag cagaaugguu aacucuauuu     1860

gaagauaaaa aaauacugcu cauaaaacuu caaaacucaa aauauaguua ugaccaagca     1920

acaaucaaua agcucucaac aaugcgauau caaggaacag gaaaacuuag caaaaaauug     1980

uuaguugauu uaaaaacuac aaaaaaaagu auuggaaaau ccggcgccga aucccuauca     2040

auacuagauc ucauguggag caccaaggac aacuucaugc aaaucauuca cgacgcagac     2100

uacaccuucg aacaacaaau aaaagaauuu aacuacgaua cagaagacga acuaacaccu     2160

cuagaaaaag uagcaaaucu gcauggaucg ccagcccuaa aaagaggacu caaucaauca     2220

aucaagguag uagcagauau aguaaaauuc augggacaug auccagaaaa aaucuuuaua     2280

gaauuuaccc gaagcgauga uuuuuccaaa uuaacaauaa gcagauaccg ucguaucaaa     2340

aaacaauacc ucgaaauugc aaaagcaauc aagaaaaucc cagccgaauu uaaggacaua     2400

aaagaguacc aaacacaacu agaagaaaac aaggguaaau uagcaaguga acgguuaaug     2460

uuguacuucc uacaaugugg acacucgcua uauucaaaua aaccaauuga cuuaaacaug     2520

auuaacucau caaaauauca cguagaccac aucuuaccac aaucauacau aaaagacgau     2580

ucauuagaaa auaaagcauu gguauuggcg agugaaaaug aaaacaaaau agacaacaug     2640

cucauuuccc augacauaau agccaccaau cuaccuagau ggcaagcguu aaaagaucaa     2700

aaccuuaugg gaagcaaaaa guucgccgac cucacucgua caacaguaac ugaaaaccaa     2760

aaaaaaggau uuauacagag acaacuagua caaacuagcc agauugucaa aaacauaaca     2820

cuuaucuuaa augauuuaua caagaacaca ucauguauag agacaagagc aacucucucc     2880

ucagaauuua gaaaagcuuu cucuaacuuc gacgaaacaa ccuaccauua ccaauuccca     2940

gaauucguua aaaacagaga cgucaacgau uuccaucacg cacaagaugc auuccuugcu     3000

uguguaaucg gugaauacca auuaaaaaaa uauccgaaag auaaucuaag acuaguuuau     3060

gaucaauaca guaaguuucu cgauucauug aaaaaagaca ccagaaagaa aaacggcaga     3120

augccaagau acacccaaaa cggauuuauu auuggaucaa uguuuaacgg caaaacauau     3180

gucgacgaua acggcgaaau aauuugggac caaaaaauca aagaauccau uagaaaaacu     3240

uucaacuauc accaauuuaa cguaguacgu caaaccaucg aacaacaugg caaauuauuu     3300

aacgauacua uacaaccgca uucagaucgc uacaaacuaa uacccuuaaa aacuaacaga     3360

gacccggcua uauacggagg uuauaauaac gacaacaaug ccuauucugu cguccuggac     3420

guugacggaa aaaaaaagau caacggaauu ccuauaagaa uugcaaacca guuaaaauca     3480

gaugaauuag accuuuccuc augguuagaa aacaauauua agcauaaaaa accaaugacu     3540

auccucaucg auaaaguucc aaaauaucag cguauaauaa acgaagaaac cggugaccua     3600

cuuauaacca gcgcaaauga aguuauaaau aacguucaau uguuccuacc auccauguac     3660

acugcacuua ucucacuacu agacagcaca aaaacagaaa uguauucaaa guuacuaucc     3720

aauuacgaag caaacauacu cauagauauu uaugacuauu uauuaacaaa acugaaaaac     3780

aacuaucccc ucuaucgcaa agaaugggcu aaauuagcag aacacagaga ugauuucaua     3840

gaaucagacc uagugacaca ggcgagcacu cuacaacaac ucauaaaauu uaugcacgcc     3900

gaccccucaa augucaaccu uaaauucggg aacuucaaag gaaaucgcuu uggccgaaaa     3960

aacggaaaua uaaaacucag caaaacagac uucauauacg aaagcccaac uggcuuguuc     4020

aaauccauaa aacauauaga uuga                                            4044


<210>  5333
<211>  4044
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5333
augaaaagcg aaaagaaaua cuauaucggu cucgauguag guacuaacuc uguagggugg       60

gcuguaaccg augaauuuua uaauauccug agagcuaaag ggaaagauuu augggguguc      120

agguuauucg aaaaagccaa aacagcggcu gacacaagaa ccuuccgcag cgguagaaga      180

agaaaugacc gaaaagguau gcgcuuacaa aucuuaagag aaauuuuuga agaugaaauc      240

aaaaaagucg acaaagauuu uuaugacaga cuagaugaau caaaauuuug ggccgaagac      300

aaaaaaguuu caggcaaaua cagccuauuc aacgacaaaa acuuuaguga uaaacaauac      360

uucgaaaaau ucccaacaau auuucaccua agaaaauauc ucauggaaga acacgguaaa      420

gucgacauac guuacuauuu ccuugcaaua aaccaaauga ugaaaagacg aggucacuuc      480

uuaaucgacg gccaaauaag ucacguaaca gacgacaaac cacuaaaaga acaacucaua      540

cuacugauua augaucuacu aaaaauagaa uuagaagaag aauuaaugga uaguauauuc      600

gaaauacuag cugauguuaa cgaaaaacgc accgacaaaa aaaacaacuu aaaagaauua      660

auaaaagguc aagauuucaa caaacaagaa ggaaacauac uuaauucaau cuucgaaucu      720

auagugaccg gaaaagcaaa aauaaaaaau auaaucagug augaagacau acuagagaaa      780

aucaaagaag auaauaaaga agauuucgua cuuaccggcg auaguuacga agaaaauuua      840

caauauuucg aagaaguccu ucaagaaaac auaacacuau uuaacacccu aaaauccacc      900

uaugauuucg uuauauuaca auccauccua aagggaaaaa guacauuauc ugacgcacaa      960

guugagagau acgacgaaca caaaaaagac cuagaaauac uaaaaaaagu aaucaaaaaa     1020

uaugacgaag auggaaaacu cuucaagcaa guauucaaag aagacaaugg uaaugguuac     1080

gucuccuaca ucggauacua ucucaacaaa aacaagaaga uaacagcuaa aaaaaaaaua     1140

ucaaauauag aauuuacuaa auauguaaaa ggaauacuag aaaaacaaug ugacugugaa     1200

gacgaagccg uaaaauaccu cuuaggaaaa aucgaacaag aaaacuuccu auuaaaacaa     1260

auaucaucaa uaaauagugu aauaccacau caaauucauu uauucgaacu ggauaaaaua     1320

cuagaaaacc uugcaaaaaa uuaccccagc uucaauaaua aaaaggaaga auucacgaag     1380

auagaaaaaa ucagaaagac uuuuaccuuu agaauaccgu auuauguagg uccgcuuaau     1440

gauuaucaca agaacaacgg cgguaaugca uggauauuca ggaauaaagg ggaaaaaauu     1500

cgaccaugga acuuugaaaa aauaguugac cuacacaaau cagaagagga auucaucaaa     1560

cgaaugcuaa aucaaugcac uuaucuccca gaagaaaccg uccuaccaaa auccagcauc     1620

cucuacucgg aauacauggu cuuaaacgag uuaaacaacc uaagaauaaa aggcaaacca     1680

cuagacacag acgugaaauu aaaacuaaua gaagaacuau ucaagaaaaa aacaaaagua     1740

accuuaaaaa gcauaaggga cuacaugauu cgaaacaacu ucgcagauaa agaagacuuu     1800

gacaacagug aaaaaaaucu agaaauaguu uccaauauga aaaguuacau agacuucaac     1860

aauauccugg gagacaaauu cgaacuagaa auggucgaag accucauuga aaaaauaaca     1920

auacauacag gaaacaaaac ucuccuuaaa aaguacauag aagaaacaua ucccgaccuc     1980

ucauccucac aaauccaaaa gaucauuaau cucaaguaua aagacugggg ccgacugagu     2040

cguaaauuac uugacgggau aaaaggccgu aacaaaacug acgacguaaa caccguaauc     2100

aacuuuauga gaaacucuag cgacaaucuc augcaaauaa ucgaaggaaa aaauuacucc     2160

uuuaacgagu auauugacaa ccuaaggaag aaauacauac cacaagaaau aagcuacgaa     2220

guuguggaaa aucuauacgu aucaccaucc guaaaaaaaa ugauuuggca aguaauuaga     2280

guaacagaag aaauaacaaa aguaaugggc uacgacccag auaaaauauu uauugaaaug     2340

gccaaaucag aagaagagaa aaaaacuacu auuucacgaa aaaauaaacu cuuagaccua     2400

uauaaagcaa uaaaaaaaga ugaaagagau ucacaauaug aaaaacuacu aacuggauua     2460

aacaaauuag acgaaucaga auugcguagu aaaaaacuau accuauauua uacucagaug     2520

ggaagggaua uguauacagg ugaaaaaaua gacuuagaca aacuauuuga uuccacacac     2580

uaugauaaag aucacauaau accacaauca augaaaaaag acgacucaau aauaaacaac     2640

uuaguuuuag uaaacaagaa cgcuaaccaa acuacuaaag gaaacaucua cccaguaccc     2700

uccucaauuc gaaacaaucc agauauuuac aauuacugga aauaccuaau ggaaaaagaa     2760

uuuauuagua aagaaaaaua uaacaggcua aucagaaaca ccccauuaac gaaugaagaa     2820

cucggaguuu ucauaaauag acaacuagua gaaacgcgcc aaagcacaaa ggcaauaaaa     2880

gaauuauucg aaaaauucua ccagaaaucg aaaauaauac caguaaaggc aucgcuggcc     2940

agugaucucc gaaaagauau gaacacacua aaaagcagag aagucaacga ccuucaccac     3000

gcccacgacg cauucuuaaa caucguagca ggagacguau ggaacagaga auuuacaucu     3060

aaucccauaa acuaugugaa cgaaaaccgg gaaggagaua aagugaaaua uucacuuucu     3120

aaauauuuua cuagaccaag gaaaucaaaa ggaaaaguga uuuggacccc cgagaaaggu     3180

agaaaacuca uaguagauac acucaauaaa ccaucagugu uaaucucaaa ugaaagucau     3240

guaaaaaaag gagaacuauu caaugcaaca auaucgggaa aaaaagacua caaaaaaggg     3300

aaaauauacc uaccucuaaa aaaagaugau agacuaaaag acgugaguaa auacggaggc     3360

uacaaggcua uaaacggagc guucuuuuuc uuaguagaac auaccaaauc aaaaaaaagg     3420

auccgaagca uugagcucuu uccacuacac uuacuaucaa aauuuuauga agacaaaaac     3480

acaguacuag auuacgcaau caacguacua caauuacaag acccuaagau aaucauagac     3540

aaaauuaauu accgaacgga aauaaucaua gacaauuucu cguaucuaau cuccacuaaa     3600

ucgaacgaug gaucaauaac cguaaaaccc aacgaacaaa uguacuggag aguugaugaa     3660

auaucaaacc ucaaaaaaau agaaaauaaa uacaaaaaag augccaucuu aaccgaagaa     3720

gacagaaaaa uaauggaauc cuacaucgac aaaaucuacc aacaauucaa agcagguaaa     3780

uauaagaacc gcagaacaac cgacacaauc auagaaaaau augaaauuau cgacuuagac     3840

acauuagaca acaaacaacu auaccaauua uuaguugcgu uuauaagucu aucauacaaa     3900

accucgaaca augccguaga uuucaccguu auaggccucg guacggaaug cggcaagccg     3960

agaauaacaa accucccaga uaauacauau uuaguguaua aaaguauaac aggcaucuau     4020

gagaaaagga uccggaucaa auga                                            4044


<210>  5334
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5334
augaaaaagc ccuauacaau cgcacuagau auuggcacaa acagcguggg auggguaguu       60

gucaccgacg acuaucgagu accuacaaag aagaugaagg ugcucggaaa uacagaaaga      120

aaaacgauua aaaaaaaccu aauaggagcu cuucuauucg acuccggcga uaccgcagaa      180

gggacccgau uaaagcgaac cgcaagaaga agauacacca gaagaaaaaa uagacucaga      240

uaucuaaaag aaauauucac agaagaaaug gcaaaagucg augacggauu cuuccaacga      300

cucgaggaua guuucuaugu gcucgaagac aaagaaggca acaaacaucc uauauucgcu      360

aauuuagcag acgaagucgc uuaccacaaa aaauacccca cgauauauca ucucagaaaa      420

gaacuagucg acaacccaca aaaagcugau cuaaggcuca uauaucuugc cgucgcacau      480

auuauaaagu uucguggcca cuuccuaaua gaagguacau uaagcucuaa aaauaacaau      540

cuccaaaaaa gcuucgauca ccuaguagac acauauaauu uacuauuuga agaacaacgc      600

cuacugacug agggaauaaa ugcaaaagaa uuacucagcg cagcacucuc aaaaaguaaa      660

agauuagaaa accuaauaag ucuuauacca ggacaaaaaa aaacaggcau auuuggcaac      720

aucauagcau uaagccuagg acuaacaccg aauuucaaag ccaauuucgg acuaaguaag      780

gacguaaaac uacaacuggc aaaagacacc uaugcagaug aucucgauuc acuacucgca      840

caaaucggag aucaauacgc agauuuguuc cuugccgcca aaaauuuauc ugaugcgauu      900

uuacuaagcg acauacucac agaaagugau gaaaucacaa gagcaccucu aucugcaagu      960

auggucaaaa gauacagaga acaccacaaa gaucuaguga cucucaaaac acuaaucaaa     1020

gaucaacucc cagaaaaaua ucaagagauc uuucuggaua aaacgaaaaa uggauaugcc     1080

ggauacaucg aagggcaagu aucacaagaa gaauuuuaua aauaccuuaa accuauccua     1140

gcccgauuag auggauccga acccuuauua uuaaaaauag aucgagaaga cuuuuugagg     1200

aaacaaagaa cauucgauaa cgguucaaua ccccaccaaa uccaccuaga agaacuacau     1260

gcaauauuac gaagacaaga aguguuuuau ccguuuuuaa aagacaacag gaaaaaaaua     1320

gaaucacuuc uaacauucag aauucccuac uacgucggac cacucgcacg aggucauagc     1380

agauuugccu ggguuaaaag aaaauuugau ggagcaaucc gaccauggaa cuucgaagaa     1440

auaguggacg aagaagcuuc ugcacaaaua uucauagaga aaaugacaaa aaacgaucuc     1500

uacuuaccaa augaaaaagu acuaccaaaa cacucccuau uguaugaaac cuuuaccguu     1560

uauaacgaac uaacaaaagu gaaguacgca accgaaggua ugacaagacc gcaauuucuc     1620

ucugcagacc aaaagcaagc aauaguagac uuguuauuua aaacuaacag gaaagucaca     1680

guaaaacaau uaaaagaaaa cuacuucaaa aaaauugaau guugggacag uguagaaauu     1740

acuggcgugg aagacucuuu uaacgccucc cuuggcacau aucaugaucu acucaaaauu     1800

auacaagaua aagauuucuu agauaaucca gacaaucaaa aaaucauuga ggauauaaua     1860

cuaacacuca cuuuauucga agacaaaaag augauaucga aacgacuaga ucaauaugcu     1920

cauuuauucg acaaaguagu cuuaaauaaa cuagaaagac accacuacac aggcugggga     1980

agauuaucag gcaaauugau aaauggcauc agggauaaac aaaguggaaa gacuauauua     2040

gauuuuuuaa aagcagaugg guucgcuaac cgaaauuuua ugcaauuaau acaugacagu     2100

gaauuaucgu uuauagacga aauagcaaag gcacaaguaa uagguaaaac agaguauuca     2160

aaggauuuag uaggaaacuu agccggaucg ccagccauaa aaaaagguau aucucaaacc     2220

auuaaaauug uagacgaguu agucaaaaua augggcuauc ucccacaaca aaucgucauu     2280

gaaauggcua gggaaaacca gacaaccgcc caaggaauaa aaaacgcccg ucaaagaaug     2340

agaaaauuag aagaaaccgc aaagaaauua ggauccaaca uacuaaaaga acacccgguu     2400

gauaacagcc aacuccaaaa ugauaaacga uaucuauauu accugcaaaa uggaaaagau     2460

auguauaccg gugacgaucu agauauagau uaccuaaguu cauaugauau agaccacauc     2520

auuccacaau cguucauaaa aaauaauagu auagauaaca aaguacugac cucucaaggu     2580

gccaaccgcg gaaaacuaga caauguaccu agcgaggcaa uaguucgaaa aaugaaaggc     2640

uauuggcaau cuuuacugag agcgggugca auaagcaagc aaaaauucga uaaucuaaca     2700

aaagcggaaa gaggaggacu aacccaggug gauaaagcug gcuucaucca gcgacaacug     2760

guagagacac gacaaauaac aaaacacgua gcacaaauuu uagauagcag auucaacacu     2820

gaauuugaug accacaauaa aagaauucga aaaguacaca uuaucacacu uaaaucuaaa     2880

cucguuagcg auuucaggaa agaguuugga cuauauaaaa uacgagacau aaaucacuac     2940

caucaugccc augaugccua ccuuaacgcc guaguagcaa aagcaauauu agguaaauau     3000

ccccaauuag caccagaguu uguauacggc gauuacccaa aauacaacuc auucaaagaa     3060

agacaaaaag cuacccaaaa gaugcuauuc uacucuaaua uacuaaaauu cuucaaagau     3120

caggaaucuu uacacgugaa uucagacggc gaagaaauau ggaacgcaaa uaaacaucuu     3180

ccaauaauaa aaaacguccu uucaauacca caaguaaaca ucguuaaaaa aacugaagua     3240

caaacaggug gauuuuacaa agagucaaua cuuucaaaag guaacucaga uaaacucauc     3300

ccaagaaaaa auaacuggga cacaagaaaa uacgguggau uugacagccc cacaguggca     3360

uacagcguuc uagugauagc aaaaauggag aaaggcaaag caaaaguccu aaaaccagua     3420

aaagaaaugg ucggaaucac aaucauggaa cgaacugcau ucgaagaaaa cccagucguc     3480

uucuuagaag cacgcggaua ucgagaaaua caagaacacc ucauuaucaa guugccaaaa     3540

uacucacuau ucgaauuaga aaacggaagg agaagacuac uagcauccgc aucagaacuc     3600

caaaaaggaa augaacuauu cuuaccagua gacuacauga cauuccucua cuuagcagca     3660

cacuaucaug aacuaaccgg cucaagcgag gacguccuac ggaaaaaaua cuuuguagac     3720

agacaccuac acuacuuuga cgacaucaua cagaugauua acgacuucgc agaaagacau     3780

auauuagcaa gcucuaaucu agagaaaaua aaucacacuu accauaauaa uagugacuua     3840

ccagugaaug aaagagcgga aaacauaauu aacguauuua cauucguagc ccuaggugca     3900

ccagcagcau uuaaauuuuu cgacgccacg aucgauagaa aacgauacac aucuacaaaa     3960

gaagucuuaa augccacauu aauucaccaa ucaguaacag gacucuauga aacaagaaua     4020

gaucucucac aauuaggcga aaauuga                                         4047


<210>  5335
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5335
augaaguccg aaaaaaaaua cuacaucggu cuagacguag ggacaaauag cguagggugg       60

gcaguaaccg augaauuuua caauauacua cgagcuaaag gaaaagaccu auggggcgug      120

cgcuuauuug agaaagcaaa aaccgcggcu gauacaagaa cuuuuagauc uggacgacgc      180

aggaacgaua gaaaaggagu acgacugcaa auccucaggg agauauuuga agacgaaaua      240

aaaaaaguag acaaagauuu uuaugacaga cuagaugaau caaaauuuug ggccgaggac      300

aaaaaaguaa gcgguaaaua uagccucuuu aacgacaaaa acuucuccga caaacaauac      360

uucgaaaaau ucccaaccau uuuucaucua agaaaauauc uaauggagga acauggaaaa      420

guugauauac gcuacuacuu ccuagcuauc aaccaaauga ugaaacgaag aggacauuuu      480

uuaauagaug gccaaaucag ccacguaaca gacgauaaac ccuuaaaaca acaacuuaua      540

cuccucauca acgaucugcu aaaaauugaa cuagaggaag aauucaugga cucaauuuuc      600

gaaauauuag ccgacguaaa ugaaaagaaa acugaaaaaa aaaacauauu aaaagaacug      660

auaaaugcac aagacuuuaa caaacaagaa ggaaaaaucu uauacucaau auucgaaucg      720

auagugaccg ggaaagccaa aauaaaaaac auaauuagug augaagauau auuagagaaa      780

auaaaagaag auaauaagga agauuuugua uuaacaggag auucauauga agaaaaccuc      840

caauacuuug aagaaguccu acaagaaaac auaacacuuu uuaacacauu aaaaucagca      900

uacgauuuug uaauauuaca aucaauauua aaaggaaaau caacacucag cgaugcccaa      960

guugaacgau acgacgaaca uaaaaaggac cuagaaauac ugaaaaaggu aauaaaaaaa     1020

uacgacgaag acggaaaacu auucaaacaa gucuucaaag aagacaaugg caauggauau     1080

guuaguuaca uuggauauua uuugaauaaa aauaaaaaaa uuacugcgaa aaaaaaaaua     1140

ucgaacauug aauucacaaa auaugucaaa gguaucuuag aaaaacaaug cgacugugaa     1200

gacgaagacg ucaaauauuu acucggaaaa aucgaacaag aaaacuucuu acugaaacaa     1260

aucuccagua uaaacagcgu aauaccccau caaauacauc uuuucgaguu agacaaaauu     1320

cuagaaaauc ucgcgaaaua cuauccaagc uuuaauaaca aaaaagaaga auucacaaaa     1380

auugaaaaga ucagaaaaac cuucacauuc agaauaccau auuacgucgg accacuaaac     1440

gauuaccaca aaaacaacgg cggcaacgca uggauauuuc guaauaaagg agaaaaaaua     1500

cgcccaugga acuuugaaaa aaucguagau uuacauaaaa gcgaagaaga auuuauaaaa     1560

cgcaugcuca aucaaugcac auaucuaccc gaagaaacug uacuaccuaa aucauccaua     1620

cuauauucag aauacauggu cuugaacgaa cuaaacaacc ucagaauaaa cggaaaacca     1680

auugacacug auguaaaacu aaagcuaaua gaagaauugu ucaaaaaaaa aacgaaagua     1740

acacuaaaau cuauuagaga cuacaugaua aggaacaauu ucgccgacaa ggaagacuuu     1800

gacaauucag aaaaaaacuu ggaaauagua ucaaacauga aaagcuacau agauuucaau     1860

aacauacuag gcgacaaauu ugaauuagaa augguagaag auuugauuga aaagauuacu     1920

auacacacag gaaauaaaaa acuacuaaaa aaauauauag aagaaacaua cccagacuua     1980

ucaagcucac aaauacaaaa aauaauaaac uuaaaauaca aagauugggg ucgacucagc     2040

agaaaacuau uggacggaau aacaggcacc aauauagaaa caggcgagau cgacacaguu     2100

aucaacuuuu ugagaaacag uuccgacaau cuaaugcaaa uaauaggaag ccaaaacuau     2160

ucauucaaua acgauauaga caaacuacgu aaaaaguaua uaccccaaga aaucagcuau     2220

gaagucguug aaaauuugua uguaagucca aguguaaaaa aaaugauaug gcaaguaaua     2280

agaauaaccg aagaaauuac aaaaguuaug ggcuacgacc ccgacaaaau auucauagaa     2340

auggcaaaaa gugaggaagg aaaaaaaacg acaauuagua gaaaaaacaa auuguuagca     2400

cuguacaaag cuauaaaaaa agaugaacga gauucacaau acgaaaagcu acuaacaggc     2460

cuaaauaaac uugacgauuc agaccuucga agccgaaaac ucuaucucua cuacacacaa     2520

augggacgcg auauguacac uggcgaaaaa aucgaucuag auaaauuauu cgacaguaca     2580

cacuaugaua aagaccacau caucccacag aguaugaaaa aagacgacuc uauuauaaac     2640

aaucuaguac uuguaaacaa aaacgccaac caaacaacca aaggaaauau auaccccguu     2700

ccuucaagua ucagaaauaa cccuaaaaua uacaacuacu ggaaauaccu gauggaaaag     2760

gaauucauau cgaaggaaaa auauaaucgu uuaaucagaa acaccccacu uacaaaugag     2820

gagcucggcg gauucauaaa uagacaacug guggaaacca gacaaucuac aaaagccaua     2880

aaagaacuau ucgagaaauu uuauaaaaaa ucuaaaauaa ucccaguaaa agcuauccua     2940

gccucagacu uacgcaaaga caugaacacc cugaagagca gagaagucaa cgaucuacac     3000

cacgcucacg acgcguucuu aaacauaguu gcgggagacg uauggaacag agaauucaca     3060

uccaacccca uuaacuacgu uaaagagaac agagaaggug auaaagucaa auauagcuua     3120

ucaaaauacu uuacaagacc ccgaaaaucc aaagguaaag uuauauggac accggaaaag     3180

gggagaaaac uuaucgucga cacacuaaau aaacccagug uacuaauuuc caaugaaagc     3240

cacguaaaaa aaggcgaacu auucaacgca acaaucgcgg gaaaaaaaga cuauaaaaaa     3300

ggaaagauau aucuaccgcu aaaaaaagac gacagacucc aagacguaag uaaguaugga     3360

gguuauaaag caauaaacgg agccuuuuuc uuccuuguag aacauaccaa aagcaagaaa     3420

cgaauacgau caauugaacu auuuccucua caucuacuau caaaauucua ugaagacaaa     3480

aacacgguac uggacuaugc aaucaacgua cuccaacugc aagacccaaa gauaaucaua     3540

gauaagauaa auuaccgcac agaaauaauc auugacaacu uuagcuacuu aauaucaacc     3600

aaaagcaaug auggaucuau aaccgucaaa ccaaacgaac aaauguauug gaggguagau     3660

gaaauuucaa aucuaaagaa aauagaaaac aaauauaaga aagacgcaau acucacugaa     3720

gaagacagaa aaauaaugga aagcuacauc gauaagaucu aucagcaauu caaggccggg     3780

aaauauaaaa aucgacgaac aacagauacu auaauagaga aauaugaaau cauagaucuc     3840

gauacacuag acaacaaaca acuuuaccaa cuucucguag cauucauauc cuugucauau     3900

aaaacaagca auaacgcagu agacuucacu guaauaggac uuggaacaga augcgggaaa     3960

ccacgaauaa cuaaccuacc cgacaacacu uaccucguau auaaaucaau cacaggaaua     4020

uacgagaaaa ggauaaggau aaaauga                                         4047


<210>  5336
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5336
augaaaucag aaaaaaaaua uuacaucgga uuggacguag gaacgaacag uguagggugg       60

gcaguuacug acgaguucua caauauacuu agggcaaaag gaaaagaucu auggggcgua      120

agacuauucg aaaaggcaga uaccgcagca aacacaagaa ucuuucgauc ggggagacgc      180

agaaacgaua gaaaaggaau gcgacuacaa auacuucgcg aaauauucga agacgaaaua      240

aaaaaaguag acaaagauuu cuaugacaga uuagaugagu cgaaauucug ggccgaagac      300

aaaaaaguca guggaaaaua uucccuauuc aaugauaaaa acuuuucaga caaacaguac      360

uuugagaaau uuccgaccau auuccaucuc cgaaaauauc uaauggaaga acaugggaaa      420

gucgauauac gauacuauuu cuuagcgaua aaccaaauga ugaaacgaag aggucauuuc      480

cucauugaug gacaaauuuc acacguaacg gaugauaagc cacucaaaga acaacucaua      540

cuauuaauua augauuuauu aaaaauagaa cucgaagaag aacuaaugga uucuauuuuu      600

gaaauauuag cggacguaaa cgaaaagagg acugauaaaa agaacaaccu aaaagaauua      660

auaaaagguc aagacuucaa caaacaggaa ggaaacauuu ugaauagcau auuugagucu      720

auagucaccg gcaaagcaaa aauaaaaaau aucauuucag acgaagacau cuuggaaaaa      780

auaaaagaag auaacaaaga agauuucguc cuaacaggcg acagcuacga ggaaaaucuc      840

caguacuuug aagaaguguu acaggaaaac auuacauuau uuaacacccu aaaaucaaca      900

uaugauuuuu uaauacuaca aucaauccuu aaaggcaaaa gcacacucuc ggacgcacaa      960

gucgaaagau acgacgaaca caaaaaggau uuagaaauau uaaagaaggu uauuaaaaaa     1020

uacgaugaag augggaaacu auucaaacaa guauucaaag aagacaacgg uaacggguau     1080

guaucuuaca uaggauacua ucuaaauaaa aauaaaaaaa ucacugcaaa aaaaaaaauc     1140

ucaaacaucg aauucacaaa auauguaaaa ggaauacuug aaaaacagug ugauugugaa     1200

gaugaagaug ucaaauaucu ccuugggaaa auagagcaag agaauuuucu acugaaacaa     1260

auaagcucaa uuaauagugu caucccgcac caaauucauc uuuucgaacu ggacaagauc     1320

cucgaaaacc uagccaaaaa cuauccauca uuuaacaaua aaaaagaaga guucacaaaa     1380

auagagaaaa ucagaaaaac cuucacguuu aggauaccau acuauguugg accauuaaac     1440

gacuaucaca aaaacaaugg aggaaaugca uggauauuca gaaacaaagg cgaaaaaaua     1500

agaccgugga auuuugaaaa aauuguagac uuacacaaau cggaagaaga auucaucaaa     1560

cgcauguuaa accaauguac auauuuaccu gaagaaacag uucuaccuaa aucaagcaua     1620

cuuuauagcg aauacauggu acucaacgaa cugaauaacu uacggaucaa uggaaaacca     1680

cucgauacag auguaaaacu caaacuaauc gaggaacuuu uuaaaaaaaa aacaaaagua     1740

acauuaaaaa gcauuagaga uuauaugguu agaaacaacu ucgccgacaa agaggauuuu     1800

gacaacucag aaaaaaaccu agaaauugca agcaacauga agagcuacau ugacuucaac     1860

aacauccuag aggauaaauu ugauguagaa augguagagg auuuaauaga aaaaauaacg     1920

auacacacag ggaauaaaaa acucuuaaaa aaauacauag aagaaaccua ccccgacuua     1980

agcagcaguc aaauacaaaa aauaauaaau uuaaaauaca aagacugggg gaggcugagu     2040

agaaaauuac uagacggaau aaaaggaaca aagaaagaaa cugaaaaaac agauacagua     2100

auaaacuucc uacgcaauag cucugacaau uuaaugcaga uaauagguuc acagaauuau     2160

aguuucaacg aguauauaga caaacugcga aaaaaauaua ucccacaaga aauaaguuac     2220

gaagucguag aaaaucuuua cguaucacca ucgguaaaaa aaaugauuug gcaaguaaua     2280

agaguaacag aagaaauaac uaaaguaaug ggauacgacc cagacaaaau auucauagaa     2340

auggccaaau ccgaagaaga aaaaaaaacg accauaagcc gaaaaaauaa acuauuagau     2400

cuauauaaag caauuaaaaa agaugagaga gauagccaau acgaaaaacu acucacagga     2460

cucaacaagc uagaugacuc ugacuuacga agcagaaaac uguaccuuua cuauacgcaa     2520

augggcagag acauguacac uggugagaaa aucgaucucg auaaacucuu ugacucaacc     2580

cauuacgaua aagaccacau uauaccccag ucaaugaaaa aagaugauuc aauuaucaau     2640

aaccucguac uagucaacaa aaacgccaac caaacaacua aagggaacau cuacccaguc     2700

cccaguagca uuagaaacaa cccgaaaaua uauaauuacu ggaaauaccu aauggaaaaa     2760

gaauuuaucu ccaaagaaaa auauaacaga uuaauaagaa auacaccauu aacuaacgaa     2820

gaacucggag gcuuuauaaa uagacaacua guggaaacua ggcaauccac caaagcuaua     2880

aaggaauuau ucgaaaaauu cuaucaaaaa ucuaaaauua uaccagucaa agcgucacuu     2940

gcauccgacu uaagaaaaga uaugaacaca cuaaaaucaa gagaaguaaa ugaucuacau     3000

cacgcucacg acgccuuuuu aaacaucguc gcaggcgacg uguggaacag agaauucaca     3060

agcaauccca uuaauuaugu caaagaaaau cgagaaggag auaagguaaa auacucucuu     3120

ucaaaagacu uuacacgacc gagaaaaagc aaaggaaaag ucauuuggac ucccgaaaaa     3180

ggacgaaaac uuauaguuga cacacugaac aaaccaucag uacucauaag caacgaaucu     3240

cacguaaaaa aaggagaacu auucaacgcc accauagcag gcaagaaaga cuacaagaaa     3300

ggaaaaauau accuaccacu caaaaaagac gacaggcuac aagacguguc uaaauacgga     3360

ggguacaaag ccauaaaugg agcauuuuuu uuucuaguug aacacacaaa aaguaaaaaa     3420

agaauaagaa guauagaacu cuuccccuua caucuacuua guaaauucua ugaagacaag     3480

aacacgguau uggacuaugc aauaaacgua cuucaacuac aagauccaaa aauaaucauu     3540

gacaaaauaa acuauagaac agaaauaauc auagacaacu uuucauaccu cauaaguacc     3600

aaaagcaacg acggcucgau aacugugaaa ccgaaugaac aaauguacug gagaguagac     3660

gaaauuucga acuuaaaaaa aauagaaaac aaauauaaaa aagacgcuau acuaacagaa     3720

gaagauagga aaauaaugga aaguuauauu gauaaaauau aucaacaauu uaaagcagga     3780

aaauauaaaa aucgacgcac gacugauaca auaauagaaa aauacgaaau aauugacuua     3840

gacacacugg acaacaaaca auuauaccaa cuccuugucg cauuuauauc acuaucuuau     3900

aaaacuucaa acaaugcugu agauuuuaca gugauaggau uagguacgga auguggaaaa     3960

ccccgaauua caaaucuucc cgacaauaca uaccuaguau acaaaagcau aacaggaaua     4020

uaugaaaaac gaauacggau aaaauga                                         4047


<210>  5337
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5337
auggagaaaa auuacuaccu aggauuggac cuaggaucua auucaguugg augggcagca       60

acagacgaag acuauaacuu ccuaagacua aaaggcaaaa cagcaugggg cgcucgcauc      120

uuuuccgaag cuaaggacug caaagaaagg aggacauaca gaaguaauaa aagaagacua      180

gcaagacgaa aauauagauu aguauugcuc aaacaauuau ucgccgaaga aaugacuaaa      240

guugacaaga cauucuuccu ucgauuagcu aacagcaccu acugggaaga agauaaaacu      300

aaagacucug auggaaacga aauaggaaag aaccuuauuu ucaaaacaca ugaagaagaa      360

aaacaguauu acacaaaaua uccaacaauc uggcacuugc guaaggcccu caucgagaac      420

gacgaagaag cacucagcaa ccuacguuau guauauuuag caauacauca cauuguaaaa      480

uauaggggaa acuuccucac agaaggcgaa augggcaacg acauaccccc aaucacaaca      540

auugaagaaa uaaaccaaac gcucagccua uuagaaguug auaguuccga agacgccacu      600

gauaaaacga cauacauaaa accugaaucu uauaacgaau uauucacaau auuagaaaac      660

aauaauuuaa acaaaguaaa gaaacaacaa gagauagaca aacuuuucau auccucgaaa      720

gaaagaucug aaaauaucaa aaacuacaua aaucuauuca aaacaauaau cguaggaggu      780

ucauuuaguu uaaagaaaau uaacgaggau acagaggaca uuagcgucga cuuuacaaaa      840

gauuucgaga acagcgaagc agacauuagc aaagcacuag gcgacuccuu caaucuagug      900

cuuaucgcaa agaaauauua cgauuacaua auccuuaaag aacuucuagg caaaaacaaa      960

gaaauauccg aagcaauggu ccaaaucuac gaagaacaua aagaugaucu aaaaucucug     1020

aaaacaaucc uaaaagaucu agauaacaaa cuaggaaaug acgguaaaga cagaauauac     1080

uacagcguau ucaaacaaga accgacggaa aaauuuaaag aaaccaauua ucuaucauuc     1140

guacaaggcg gaaagcugga agaacugaac aaauacauca agaaaauauu agaaaacaac     1200

gaaaaagaua uagacgauaa agaaacauau aaugcccuau acgaaaaagc gugcaaaggg     1260

gauuuauuua agaaacaggc uaaugcuugc acgagccuga uaccacacca auuucaucua     1320

agggaacuaa agaaaauucu cgaaaaugcg gggcguaaau acucuuucuu aaacgacaua     1380

agcgacaaaa uaauaaaaau auucaaauuc agaguaccgu auuacuacgg cccacucaac     1440

aaugaaagua augcgcacag cuggguagua aagaaagaag ggcaagaaaa agcaaagauc     1500

acaccaugga acauaaacga aaucauagau acgcccaaaa cuagagacaa cuucuucaag     1560

aaacugacca auagcuguac auacuuagua ggcgagacag uacugcccaa aacaucacuu     1620

uacuaugaaa aauaccuagu acucgacagg cuaaacaaaa ugcaaguaaa cggagauuau     1680

cuaucagcua gugagaaaaa cgacguauug aaauucauac ucucccguaa aacuacaacg     1740

auagaacaaa uaagaacaua ccucagcaaa aacaaaucaa cuaaagcaaa cgacauaagc     1800

aucagcaaca uaaaagcgac ugauccuuuc aacgcaguuu cucacgcauu uuacggaaaa     1860

cacccggauc ucaauauaaa uuuaugcgaa gaacugauca aauucgcaac aauauacgca     1920

gacgauaaga aagaacucaa gaaauuuguc ucaaguaacu uuaaucuaac ugaugaguuc     1980

auaaaagaau acuugaauac accuacaaac aaauggggua gacuuuccag aaaauuccuc     2040

ugcgguggag accaauuaga aauguauuau gucgacgaca acggcauauu acauagcauc     2100

uugaacauuc uagaagaaac aaaugaaaau cuacaagaaa uccuaaacaa ugaaaaauac     2160

ggguucaacc agaaaauagc agacuucaac aauacucuua cagcagacaa aacaacagaa     2220

gaacaaauag aagauaucuu ggacuccaca ccagcauuaa ugagaagauc aauuauacaa     2280

acacuugcgg uaguagacga cguaauuaaa gcagcaggaa aggcaccaca gaaaauaaug     2340

auggaaguaa cacgcacuga cggcaagaaa gaagaaacaa aaucaaggua cguacaacuc     2400

aagaaacaaa uaacaaacga uaaaguauua acaucagacg aaaagaaaga acuauuaacu     2460

aacuuagaaa acaagaaaau ccaacucaaa ggaaaacaua uauaucuaua cuucaugcaa     2520

auggguauag auuuuuauac aggaaaaaua auggacauaa acgacguaau aaauggaaaa     2580

uaugauauug accauaucau uccacaauca augaucaaag augacagucu cgacaauaug     2640

gugcuaguaa gcaaagacua uaaccaaaaa guuaagaaag auaucuaucc aauaugugac     2700

gaaauacgua auaacaugcu agacaaaugg aaaggcuacu acgccaggaa aauuauuucc     2760

gaagaaaaau acaacagauu aaaaagaaca aaagaacuca augcagacga gauaaacgcc     2820

uucaucaaua gacaaauaaa cguaguuaau uacucaaaca uacaaaucaa aagaauauuu     2880

gaaauaaaau accccgaaac aaaaauaaua uucuccaaag ccagcuuucc uagcuuacuc     2940

cguaaagaau acgaaauagc uaaaaugaga gauuuaaaug acgcacacca cgcuguugac     3000

gcauaccuaa acauaguuug cggaaauaua cucacgacau uauauacgga caucuucuau     3060

auguucaaag aaaagaaaga agaccacaaa aacaucucau ucaacaugga gaaaagacuu     3120

auaaacuaua uagauaaaaa caacuuaaaa gacaaaauaa aaacaagcug cuuacgacac     3180

gacgcacuca uaacauauaa acccgacuac gacagcggua aauucuacaa agaaacaaua     3240

uuuaagaaaa gcggagauaa agaaaccgga uuaauaccgc uccauacaaa aggacccaug     3300

agugacacua agaaauaugg aggauacuca caauuaucgc aauccuauau aauagcgguu     3360

gaauacaaac ucaaaaauaa aacaguaaag aaauuauguc gaguucccac cuuauaccua     3420

aaacaauaca aaaaugaccu aaauacgauc gcaaaaauca ugauagacga cgaaaaagcu     3480

acagacauca aaguacucag aaaaauuuac cuaaaccaga aaaucaggua caacggaugc     3540

uacuaccuaa ucuauacauc uaaagaaaaa caaaauaaau auaaaauggc auaccaaaac     3600

uaccuagaca augauuaucu aauauaccua aacaaauguc ucaagaaaau agaugaacua     3660

gacgaugggg aauuaacaag ucaagaauuu auugucaaca aagacggcga caaauuucuu     3720

auagacaaaa aucacaaucu ggaaauuuuu aaaucaauca aagaacaaua caacaaaacc     3780

auauaugauu acacacccua uauaguacaa ucccgcgaaa ugaaagaaga ggacuuuaac     3840

aaucuaaguc uaaagaaaca aauagacaca uuauuaaaca ugaucaaaau acuaucaagg     3900

agcaacgaac aagcuaaauu cgaaacugcc uucuucgaaa acuucaccaa aguauuccua     3960

aaaaccauga caauaacuaa cgaaaaaguc uacauaguca acgaaagucc aucugggcug     4020

uucaaaggaa aagaagaacu aauauga                                         4047


<210>  5338
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5338
augaaaaaau ucgaagauua cuauuuaggu uuagacuugg gaacaaguag uauaggaugg       60

gcgguaacaa augagaaaua cgacaucuua agauuuaaca aaaaauucau guggggcacc      120

cacuuauuug acaugggcaa uaccgccuca gaaagacgaa uauuccguac cucuaggaga      180

agacucaaaa gaaaaaaaaa ucgaauagaa uuacuacaaa cauuauuuuc agcagaaaug      240

ucaaaaaagg auuucggauu cuuucaaaga cuuaaagaaa gucaguacuu uaucgaagac      300

aaaaccuuua aaaacaaaaa cacauuauuc auggaugaaa auuauaccga ucaagauuac      360

cacauggaau accccaccau auaccacuua agaaaagcac uaauugacaa cgaggaauca      420

gccuuugaaa ucagaaaacu uuaccucgcc auaucccaca uacucucaca ucgaggacac      480

uuccuauucg aaggugaacu aucggacaac cuagacuuug augauauauu caaugaauuc      540

guaaaauauc uaaacgaaga guuaaauaua gauuucaaaa uuaaauccga ugucucacua      600

caggcuauaa aagaaauucu cacagauaaa augcucaacc gaaccaaaaa aaaagagaaa      660

cucucagaac uucuugaaga agaaaaaaau aaaauauacc aagaaauaug gaagcuacug      720

guaggcaacg aagcuaagau gagcacaaua uucgacgaca aagaauacga agaagccaag      780

auagcauucu ccaaaggaaa uuaugaugaa aaagaagaag aauaugagag cauacuaggu      840

gaaaaaauau uauuuguaca aaaagcgaaa gcaauuuaug acuggauguu auuacaaaac      900

aucuuaaaag aagacaaaua ucugucauac ucaaaaguua aacucuacaa ugagcacaaa      960

aaagaccuaa aaauauuaaa agaugugcua aaaaaccuug acaaaaaaau cuacaaagaa     1020

auauucagcg agaauggacu auacgaagaa uaucuuagaa uugagaagaa aaagauugga     1080

ucuaaggaag aaaagacuaa agauauauua uacaaccgac ucaaaaaaau auucgaaaau     1140

agagauaaca uggccaucga agacauacuc aauaaaauug aacucgauuc uuuucuaccu     1200

aaacaaaaaa uaaaagacaa cggaguaaua cccagacaag uacaccagaa agaacuugaa     1260

auuauacuga aaaacgcuga aaaauauuuu aaauggcuaa uagaaaaaga ugaaacagga     1320

cuuuccgucu cagaaaagau aaugaaaauc uuuaaauuca aaauaccuua uuacguugga     1380

ccucuaaacu caaaaucacc auuugcaugg auugaaagaa aagaauaugg aaaaauucua     1440

ccuuggaauu uugaaaacau gguagacaug ucaaaaacgg cagagaaauu cauaaagaga     1500

augacaaaca aauguaccua uuuaccugaa aaagauguaa uaccaaaaaa cagccuacuc     1560

uauaccaaau acaugguccu uaacgaauua aacaaucuaa aaauacgugg agaaaaaauc     1620

aaaguggaac uaaaacaaaa aauauacaca gagcuuuucc aaaaaaauaa gaaaaucgga     1680

augaaaacac uaaucgaauu ucuuaaauca caaaacauag uuguaacaaa aguugaaauu     1740

acaggaauag acggcacguu uaacacauca cucggaucgu auauugauau gaaaaaaaua     1800

uucggcgaag aaaaaauagu caaagauagc uauaagaaaa ugauagaaga cauaauauua     1860

uggauuacuc uauacggaga agaaaagaaa cuaauaaagu ccaaaauuga agaaaaauau     1920

ccugaaaaag uaacaccaga agaaaugaaa uuaauauuaa aacugaaaua uagggacugg     1980

agccgacuau caaaagaauu ccucacagaa auagaaacua uggacgaaga aacaggggaa     2040

cuaauaucaa uaauagaaau gcuauggaga aaaaacgaaa auaucaugga acuguuauca     2100

uacaaauacg acuuuucaaa ccgaauaaag gaauacaaug gaauaaacga aaaagaaaaa     2160

aauauaacuu acgaagacuu agugaaagac cuacaaauaa gccccucugu aaagaaaaug     2220

auuuggcgug cacuuguaau aguaaaagaa aucaaaaaag ucacaaacaa aucaccuaaa     2280

aaggucuuca uagaaauggc ucgcgacacc caaaccgaaa agaaaagaac agagaagcgc     2340

aaagacaaac uccuagaauu auauaaaaac aucaagaacg augauaguau aaaagagcuc     2400

gaaaaaaacu uaaaagacaa aacaaacgau gaccuuaaau caaaaaaguu auaucuauac     2460

uaccuacaau uagggaggug uauguauagc ggggaaguca ucagauuaga agaccucaug     2520

aacgauaacc ucuacgauau agaucauauu uauccacaau ccaaaauaaa agaugacuca     2580

uuugauaaca ccguauuagu uaaaaagcaa auuaacgcag auaagacaaa cgaauacccc     2640

auagauaaau caauccaaaa uaaaugcaaa gcuuucugga ccucccuaaa agaacgagga     2700

cugaugucaa auacgaaaua cgaacguuua acccgaacgg aaggauucuc cgaugacgaa     2760

cucgccgguu ucauaucaag acaaauugua gaaaccaggc aagcgacgaa aguccuggca     2820

gaccuauuaa aaguaauauu cggaagugac acacgaauag uauacgucaa agcaggacuc     2880

acaucugacu uccgccaaaa auucggaaua uauaaauuuc gagaacucaa cgacuaucau     2940

caugcacacg acggauaccu aaacauugua acagggaaca ucuauaacac auuauucaca     3000

gacaauccgu acaacuucau aaaagauaga aaaaaagaua acagaacaua uaaucuuaau     3060

aaaguauuug aaagagaaaa ugacguaugg aaucccgaug ucauguugag caaaauagag     3120

agguauaucu acaaaauaag accccuauuc acucgggcaa gcuacgaaca aaaagggggc     3180

uuuagugauc aaaacauauc cucagaaaaa gacaaauuaa gaccacuaaa aucaucuggu     3240

guccuuucaa acauuaagua cggaggauac gauaaaauca aaaacucuua cuuuuucauu     3300

guggaauaca ccgaaaaaaa gaaaagggua agaaguauag aaacacuccc aaucuauaua     3360

aaaaagucaa uaaguacaaa ggaggaccuu gaaaaauauu guaaagaaga auuaaaauua     3420

caagaaccua aaguacuagu cagcaaauua aauuacaaau cccuuauaua cucaaacgga     3480

cacgaauacu auauaaacuc aaaaacagca gauuccauca caauucaaaa uggaauucaa     3540

auaauccuca cuaaagaaaa cuacgaaacg uacagaaaaa uuuacaagaa ucucgaauac     3600

uuaaaacuaa uauauaagga cgagaaaauc cgcgaagaaa aaauacucga aaaaauccca     3660

aaaauauuaa aaaacaauaa aaaugacaga gaccucucag aagaugaaau aucagagaaa     3720

uucuggaagc uauacaaaga aaucuucgaa aaaaucaaaa uuggcaucuu caguaaauuu     3780

aaauccacac ucccuauuua caucgaggau ugcaaaaagc uuaaagacuu uacaacacua     3840

aacaacuacg cuaaaucacg cuucauacaa gaaauucuaa agugccuuaa cagccaacag     3900

ggggaauucg acacaaaaua uacacauaac uacuccaaaa aauauaacac caaacucucg     3960

aaaucaaaaa uacuagaaaa cgacuucuac auaauaaacc agucgccaac aggacuuuuc     4020

gaaaaaaaag uauuccuaga uaaauga                                         4047


<210>  5339
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5339
augggagaag auggaaaacc auuaagggua aaaggaaaaa cagccauagg agcacgccuu       60

uuccaagaag gcaauccagc agccgacagg agaauguuua gaacaacacg gagaagauug      120

aaccgccgca aauggcgauu aaaauuacua gacgaaauuu uugaccccua uauaaccccu      180

guagacagca cauucuucgc acgcuuaaaa caaucaaacc uaucgccaaa agacuccaga      240

aaggaauuca aaggcucaau gcuauuuccc gaccucacgg acaaacaaua ccacuuagau      300

uaucccacga uuuaucaccu uagacacgcc uugaugacac aggacaaaca auucgacaua      360

cgaaugguuu auuuagcaau ccaucacaua gucaaauaua gaggaaauuu ucuaaauuca      420

acacccgucg acucauucaa agcuucuaag guaaacuucg augaccaauu uaaaaaacua      480

aaugaacucu auacagcaau aaacccagaa gaauccuucc aaaucaaucu agcaaacuca      540

gaaaaaauag gucagcaauu ccucgacccc ucaauaagaa aauuugacaa aaaaaaacaa      600

auuccaaaaa ucguaccagu aacaauggac gauaaagcug uagauaaauu gaauggaaaa      660

auagcaagcg aaauaauaaa cgccauacua ggcuauaaau cuaaacuaga uguagcagua      720

caaugcgacc cagaagacuc caacccgugg gcaguaaaau uugaugacga agacauagac      780

gcaaaauugc aaaaaauccu acccgagaug gacgaaaacc aacaaaguau cauaggcauc      840

auacaagauu uauacucuca aguuacucua aaucaaauug uuccaaacgg aaugucauua      900

agcgaaagca ugauaaaaaa auauaaugac caccaugacc aucucaaacu cuacaaaaaa      960

uugaucggac aaauuaguga uucaaaaaag aaaacggcuu uaaaaaaagc cuacgaccaa     1020

uacgucggca augauggcaa aguaauugag caggaugacu ucuauucgag cgucaaaaaa     1080

aacuuagaug acucugaacu aucuaaacaa aucauggacc uaauugaugc agauaaauuc     1140

augcccaagc aacgcacgag ucaaaacgga guaauaccuc accaacucca ucagcgggaa     1200

cuugacgaga uaauagaaca ucaaucaaaa uacuauccau gguuagccga guccaacccc     1260

aacaaacacg accuacaccu agcaaaauau aaaaucgaag aguuagucgc auuucgaauu     1320

cccuauuaug uaggaccaau gauaaccccu gaagaccaaa aaaaauccgg agcagcaguu     1380

uuuaguugga ugaaacgaaa agaaccagga caaauuacac cauggaacuu ugacgaaaag     1440

guugacagaa uggaaucagc aaacaaauuu aucaaacgaa ugacuacaaa agauaccuau     1500

cugaucggag aggauguauu accggacgaa uccuuacuuu acgaaaaauu uaaaguccua     1560

aacgaacuua acaugguacg aguaaacggg gacagucuaa aaguugcaga caaacaggca     1620

auuuaucaag aucuauuuga aaacuauaaa cacaucagug uaaaaaagcu acaaaauuau     1680

aucaaaggaa aaaccggacu acccaacaau ccagagauau cuggacuauc agauccagau     1740

caauucaaca auucauuggg uacauauaau gauuuuaaaa aacuauucgg agauaaagua     1800

gacgacccag acuuacagaa ugacuuugaa aaaaucgucg aauggucgac aguauucgaa     1860

gauaaaaaaa uacuuagagc uaaacucaac gaaaucacau gguuaacaga caaacaaaag     1920

gaugaccuag aaucaucacg guaccaagga uggggaagac ucuccaaaaa acuacuuaca     1980

gggaucguaa acgaucaagg ggaaagaaua auagacgaac ucuggaauac caacaaaaac     2040

uucaugcaaa uacaaucaga ugacgacuuc gcgaaaagaa uccacgaagc uaacgcugac     2100

caaaugaaaa guacaaauau ggaagaugua cuugcagacg cauacaccag uccacaaaau     2160

aagaaagcaa uccgccaagu gguuaaagua guagacgaua uccaaaaagc aaugggagga     2220

gucgcaccaa aauucauaag caucgaauuu acacgaucug aagacagaaa cccaagaaga     2280

acgauaucua ggcaacgcca acuagaaaau acacuuaaag acacagcuaa aagcuuaguu     2340

aaaagcauga acccaaacau cuuaagcgaa cuugauaaug caucuagauc aaaaaaagga     2400

cuaacagaca ggcuauaccu auacuuuaca caauuaggua aggacaugua cacuggggag     2460

ccgaucaaua uagaugaaau aaguaacuac gacaucgauc acaucuuacc acaagcauuc     2520

auuaaagacg acagucuaag uaaucgcguu uuaguaucaa aggcaguaaa uaacgguaaa     2580

ucagcuaaug ucccacuaaa auuguuuggc gcuaaaaugg gucacuucug gaaacagcuu     2640

gcagaagcag ggcuuauuaa uaaacauaaa uugaaaaacc uacaaacuaa cccagauaca     2700

auaaguaaau uugcuaugca uggguuuaua cgaagacaau uaguagaaac aucacaaguc     2760

auaaaacucg uagcaaacau cuuaggagau aaauaucgaa augacgauac aaaaauaaua     2820

gaaaucaccg cacguaugaa ccaccagaug agagacgaau uuggauuuau caaaaauaga     2880

gaaauaaacg auuaccauca cgcuuucgau gccuaccuca cggcauuuuu agggcgauac     2940

cuuuaccaca gauacauuaa acuacggccu uacuucguau auggagacuu caaaaaauuu     3000

agagaagaca aaguaacaau gagaaacuuu aacuuucuac augaccuaac ugaugacacc     3060

caagcaagaa uagcagauac cgaaacaggu gagguuauau gggauagaga aacaagcaua     3120

aaacagcuuc gagaugucua ucauuauaaa uuuaugcuaa uaucccaaga aguauauacc     3180

cuccgaggag caauguuuaa ucaaaccaua uacccagcuu cacaagcagg aagaaaaaaa     3240

cuaauuccua ugaaagcuga caaacccgug gauguuuacg gaggguacuc gggaagcaca     3300

gacgcauaca uggcaaucgu gagaauucau gacaaaaaag gagacaaaua uaaaguugua     3360

gguguuccaa ugcgagcacu uaacagauua aauacagcua aaaacguauc agacgccaaa     3420

uacgaccaag aacuaaaaga aguauuaaca ccacaauuca ccaaaucgaa aaagaaucgc     3480

aaaacaggag aaauaacaca aaccgucgaa gauuucgaag uugugcuagg uaagguuaug     3540

uacaggcaac ucguaaucga cggcgacaaa aaauucaugc uagguucauc aacuuaccaa     3600

uacaaugcga aacaacuggu acuaucugau gaaucaauaa aaacucuagc cuccgaagga     3660

aaacuuaaug aagcacaaga gucugcagca uaugacaaag uauaugacga aauccuugau     3720

aaaguaaaca aauacuucgc ccuauaugau acaaacaaau uucggcaaaa acucagucuc     3780

ggaaaagaua aauucgucgg acuaccaaau cacaacguau uugacggauc aaaaaaaauc     3840

ucaucaggaa agcgcgaaau acuuaacgaa auucuaaacg gacugcacgc gaaugcaaca     3900

cuaggcacac uuucugacau cggcuuuuca acacccuuug gaaaaaugca acaaccucga     3960

ggcaucggac ugagcucaaa uacuucuauu gaauaugaaa guuucaccgg gcuauucaaa     4020

cgaacaauau ucuugaaaga ccuauga                                         4047


<210>  5340
<211>  4047
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5340
augaagaaac cauauacgau ugcacuagau auaggaacaa acuccgucgg auggguaguu       60

gucacugacg acuaucgagu cccuacaaag aaaaugaaag uacuuggaaa cacugaaaga      120

aaaaccauca agaaaaaucu aaucggagca uuauuguuug auaguggaga cacagcagag      180

ggcaccaggu uaaaaagaac cgcaagacca agguacacaa gaaggaaaaa cagacucagg      240

uuuuuaaaag aaaucuuuac cgaggagaug gccaaaguag augauggcuu cuuucaaaga      300

cuugaagauu cauucuacgu acuugaagac aaagaaggaa auaaacaucc uauuuucgca      360

aacuuagcag augaaguagc cuaucacaag aaauauccua ccauauacca ccuaagaaaa      420

gaauuaguug acaacccgca aaaagcggac uuacgacuua ucuauuuagc agucgcacau      480

aucauuaaau ucagaggaca cuuccuuauc gaaggcaccu uaaguuccaa aaacaacaac      540

cuacaaaaau cauucgacca ccuaguggac acauauaacu uacuuuuuga agaacaaagg      600

uuacucacag aaggaauaaa cgcuaaagaa cuuuugucag cagcacuuuc aaaaucaaaa      660

agacuagaga acuuaaucuc auuaauacca ggucaaaaga aaacuggaau auucggaaau      720

auaauagcau uaagccuggg ccuuaccccg aacuucaaag caaacuucgg cuuaucaaaa      780

gauguaaaac ugcaauuagc aaaagauacc uaugcagaug aucucgauag ccuauuagcu      840

caaauaggag accaauaugc agacuuauuu cuagcugcca aaaauuuaag cgacgcgaua      900

cuguuaucag acaucuuaac ugaaucagac gaaauaacua gggccccacu aagugcgagc      960

augguuaaac gauauaggga acaucacaaa gacuuaguua cacuaaaaac acuuauuaaa     1020

gaccaauuac cagaaaaaua ucaagagaua uucuuagaca aaacuaaaaa uggauacgca     1080

ggauacauag aaggccaagu cagucaggaa gaauuuuaua aauaucucaa accgaucuua     1140

gcuagacucg acggaucaga accauuauua cuaaaaauag accgagaaga cuuucuaaga     1200

aaacaaagaa cguucgauaa ugggucuaua ccacaccaaa uccaccuaga ggaacuccau     1260

gccauacuaa gaagacagga gguauuuuau ccauucuuaa aagacaauag aaagaaaaua     1320

gaaucauuac ugacguuucg aauaccauac uacguaggcc cacuggcacg uggucauucc     1380

agguuugcau ggguaaaaag aaaauuugau ggcgccaucc gaccauggaa uuuugaagaa     1440

aucguagacg aggaagccuc agcacaaauc uuuauugaga aaaugacgaa aaacgaucua     1500

uaccuuccca acgaaaaagu acuuccaaaa cauucccuac uuuacgaaac auucacagua     1560

uacaaugaac uaacaaaagu aaaauacgcc acagaaggca ugacacgacc gcaauuucua     1620

ucggcagauc aaaaacaagc aauuguagac cuauuauuua aaacaaaccg aaaaguaaca     1680

guaaaacaau uaaaagaaaa cuacuuuaaa aagauugaau gcugggauuc uguagaaauc     1740

acaggaguag aagacucguu uaaugcauca cuaggaaccu accacgacuu auuaaaaauu     1800

auacaagaca aagacuuuuu ggauaacccg gacaaccaga aaauuaucga agauaucaua     1860

uuaacccuga ccuuauuuga agacaagaaa augauuagca aaagacuaga ccaauaugcu     1920

caucuauuug acaaagucgu auuaaacaaa uuagaaaggc accauuauac aggaugggga     1980

cgucucucgg gcaaacugau caacggcauc agagauaaac aaucaggaaa aacaauuuua     2040

gacuuccuca aagcagacgg auuugcaaau cgcaacuuca ugcaacuaau acaugacuca     2100

gaacuuagcu uuauagacga gaucgcaaaa gcacaaguca uuggaaaaac agaauauagu     2160

aaagaccucg ucggaaauuu agcaggaucu ccagcaauaa agaaaggaau aagccagaca     2220

auaaaaauug uagaugaacu ugucaaaauu augggguauc uuccacaaca aauaguuauu     2280

gaaauggcaa gagaaaacca aacuacugcc caaggaauua aaaacgcacg gcaaagaaug     2340

agaaaacuag aggaaacggc aaagaaauug gguucaaaua uacugaaaga acacccaguc     2400

gauaacaguc aauuacaaaa cgauaaacga uauuuauauu aucuccaaaa uggcaaagau     2460

auguacacag gugacgaccu agauauagau uaccuaucaa guuacgauau agaccauaua     2520

auaccucaaa guuuuaucaa aaauaauagc auagacaaua aaguucugac aagucaagga     2580

gcuaauagag gcaaauuaga caaugucccu ucagaggcga uuguaagaaa aaugaaagga     2640

uauuggcaau cgcuccucag agccggugcc auuucuaaac aaaaauuuga caaucuaacc     2700

aaagcagaaa gaggaggacu uacacaagua gacaaagccg gauucauaca acuucaauua     2760

guagaaacua gacaaauaac aaaacaugua gcacaaauac uagacucgcg guucaacaca     2820

gaguucgacg accauaauaa aagaauacgu aaaguucaca uuauuacacu caaaucaaag     2880

cucguaucag acuuuagaaa agaauuugga cuuuacaaaa uaagagacau aaaucauuac     2940

caucacgcac acgacgccua ucuuaaugca guaguagcaa aagcuauacu agggaaauau     3000

ccacaauuag cgccugaguu cguauaugga gacuacccaa aauacaacuc auucaaagaa     3060

aggcaaaaag caacacagaa aacguuguuu uauucaaaua uucuaaaauu uuucaaagac     3120

caagaaucuu uacauguaaa uucagacgga gaagaaauau ggaaugcuaa uaaacaucua     3180

cccaucauua aaaacguucu uucaauucca caaguaaaua uuguaaagaa aacggaaguu     3240

caaaccggag gcuucuacaa agaaucaaua uuaagcaaag gaaacucaga uaaacuaauc     3300

ccucguaaaa acaacuggga caccagaaaa uauggcggau ucgacagccc uacaguugca     3360

uauucaguac uuguaauagc aaaaauggaa aaaggaaaag caaaaguucu aaaaccuguc     3420

aaagaaaugg uagguauaac aauaauggaa agaauugcuu ucgaagaaaa uccagugguc     3480

uucuuagaag ccaaaggaua cagggaaaua caagaacauc ucaucauaaa acucccaaaa     3540

uacucucuuu uugaauugga aaacggaaga cgaaggcuac ucgcgagcgc cucagaacuu     3600

caaaaaggua acgaacuguu uuuacccguu gacuacauga cauucuuaua ucuugccgcu     3660

cauuaucaug aauuaacagg uucaucagaa gacguccuac gaaagaaaua uuucguugag     3720

agacauuuac acuauuucga cgacauuaua caaaugauaa acgacuuugc cgagcgacac     3780

auccuugcaa guucuaaucu ggagaaaauc aaucacacau aucacaacaa uagcgauuua     3840

ccaguuaacg aaagagccga aaauauuaua aauguauuca cauuugucgc acuaggagca     3900

ccagcggcuu uuaaauucuu ugaugccacu auagauagaa aaagauacac uucaacaaaa     3960

gaagucuuaa acgccacccu cauucaucaa aguguaacag gccuguacga aacgcgaaua     4020

gaucuaagcc aacuaggaga gaauuga                                         4047


<210>  5341
<211>  4050
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5341
augaaacaaa aauuuuacgu aggcuuagau augggcaccg cgucuguugg cugggcagug       60

accgaugaca aauacaacau uauaaaaaga cacggaaaag cacucugggg aguuagauug      120

uuuaaaacag cuaacacagc agaagagaga agaauauucc gagcagcaag gagacgaaca      180

caaagaagaa aacaaagaau ggaacuccua caagagauau ucggagacga aauaagcaaa      240

acggacauag gauuuuacca acgaaugaag gaaucaaaau acugguuuga agauaaacga      300

gacauaaaug guaaacaacc cgaacuaccg uacgcccuau uuguugauga cggauuuacg      360

gauaaagacu aucacgaaca auuuccuaca auauuucacu uaaggaacgu ccuaguaaau      420

gaauggacuc aaaaguauga uauccgacua gucuaucuug ccauucacca caucauaaaa      480

caucgcggac auuuuuuauu ugaagguaaa aaaaugagcg aaguaacaga uuuuggaagu      540

gcauuugaag cauuuuguaa agucgccuca gauaaugaca ucgagauaac uguagaugaa      600

gagaaguuaa aagacauaga agaccuacuu aaaucgagac aaaaaagcaa aacagcaaaa      660

aaacaagaac uagguaagau uauagcggga gcugacaaac aaaaaaaagc ucuggcggca      720

cuugugacag gaugcauggu uaaauuauca gaaauauucg cagauaaguc uuuagaugau      780

ggggaaagaa acaagauauc guuugcagau uccaacuacg augaauacau ugcaaacguu      840

gaagaaacgc uagcagaaag guuccuccuu auagcagcug guaaagcccu guaugacugg      900

gcagugcuau cagacauacu cgaaggauca acauacaucu cagaggccaa aguuaagauu      960

uacaaaaagc acggagacga ccuacgaaaa cuuaaaggau uauuaaaaac ucgaccagau     1020

gaauacaaac uaauuuucgg ccuaccagaa aaacaagcca auuacagcgc auauauagga     1080

augguaaaaa aaaacgguaa gaagguaccc auagauaaaa aauguacaaa agcugacuuc     1140

uauucauucc uuaagaaaac ccucaauaaa auuaauuuug aggacgaaaa ggagagagaa     1200

aucgcagaau cuaucauuaa cgaaauagag aacgaaacau uccuaccaaa acaagugaua     1260

agcgacaacg gaguuauucc uuaccaacua caugaggcag aauuagacag aaucuuaaaa     1320

aaugcagccc uauauuaucc guucuuaagc gaaaaagaug acgaggggua cacaaauaug     1380

gauaaaauaa aagcaauuuu caaauuuaga auuccauacu auguuggccc cuuaaauaca     1440

uaucauucug acaagggggg aaacucuugg gcaguuagaa aacaagaugg aaagauauac     1500

ccuuggaauu ucgaaucaaa aauugaugca ggagaaagug cacgaaaauu uauaggaaga     1560

augacaaaua aauguacaua cuuaauugga aaagauguac uaccaaaaga aagucuacuc     1620

uauuccaaau ucaugguacu aaacgaacua aauaauuuaa gaauagaugg agaaaaagug     1680

aguguagaaa uaaaacaagc aguauacgaa aauguguuua aaaaauacca gcguguuacu     1740

ggaaagaagg ucaaagacuu ucugaucaga agcggaauca ucgggagaga acaagaacuu     1800

agugguuucg accaaaauuu caagucaagc cuaaaagcau aucacgauau uaaacagauc     1860

gcaggagaca uaaaucuauc ucaaaaucaa aaagaagaca uaauaaaaga aauuacugua     1920

uucgcagaua gccaaaaaau gcuaaaaaaa cgccuaaaaa uaaaauaucc agaacuaagc     1980

gauaaacaag uaacaaaauu agcagccaaa aaauauacag gauggggccg acuaagcaga     2040

gaauuucuug aagaagucga aaguguaaac aaagaaaccg gugaaguacu caauauuauc     2100

agugcuuuau gggaaacaaa cgagaaucua augcaauuac uaaguaauaa auaugauuac     2160

cuuagugaaa uaaagagacu aaaugacgaa gccgaagaua aauccgaagu uguuacauau     2220

gacgauguac agaaauugua uguaucaccc gccguaaaaa ggccaguaug gcaaacacuu     2280

aaaaucgucg aagaaauaag uaagauaaug gguucaaagc cagaaagaau auuuguagaa     2340

auggcuagag agcccggaca aaaaggugau gucaaagucu caaggaaaag uagacuucua     2400

gaaaaguaua aauacuguaa aaaagaggca ccagaaauau acgaaaaccu cguaaaagaa     2460

gaugauaaca aguuacgauc agauaaauug uuuuuauauu auacccaaau gggcaggugu     2520

auguauagua augaagucau cgacaucgag gacuuauuca caaacagaua cgauauagac     2580

cacauauacc cacagucaaa aguaauggau gacucgcuag auaacagagu ucuaguaaaa     2640

cgcgaacuaa auucaaacaa gacagacgug uuuccagucc caaaagagua uauggauaag     2700

gcgaaaacac ucugggauau guuaaaaaaa aaagaucuua uaaguaaaga aaaauaucau     2760

agacuaacuc gaagagaugg auuuagugaa aacgaacuau cagguuucau agcaagacaa     2820

cuaguagaaa ccaggcaaag uacaaaagcc gucacacagc uacuaacgaa agcuuauccg     2880

gaaucugaaa ucguuuaugc caaagcaaac gcgguaucag aauuuagaca uaaguucgga     2940

uuuaccaaag uuagaucgau aaaugacuau caccaugcua aagacgcaua ccucaauauc     3000

guagucggaa auacuuauua ugugaaauuu acaaaagaug cagccugguu cauaagaaau     3060

aacccaggua gaacauacaa ccugaaaaag auguucgaac aaggcacggu aucuagaggu     3120

ggagaaacag cuugggucuc aggagaagac gggacuauau uuacaguuaa acgaguaaug     3180

ggcaaaaaca acauauuauu uacucgaaaa gcguaugaag uacgaggugg auuauucgau     3240

caacagauca ugaaaaaagg aaaaggacaa auucccguaa aaaacaacuc cgaagaugac     3300

cguuuaugcg cuacagagaa guauggugca uauaacaaag caucgggagc cuauuuuaug     3360

cuuguagagu cagaagauaa aaaaggaaau aaaauuagaa cauuagaauu ugucccggug     3420

uacuuagcaa aagaauugga aaagucagaa gacgcagcaa ucagauauug ugucgaaaaa     3480

cuagaauuaa aaaauccaga uguuagaguu agaaaaauca agauggauag ccuauucaau     3540

guagauggau uucgaaugca ucuaaguggc agaacuggaa aacaauuaau auucaaaugu     3600

gcagaacaac ugguccuaga cgaggaauca caaaaaaucc ucaaaaaaac ugccaaagug     3660

uucgcagacu auaaagagaa uaagaacauc aaaaucuccg acaaaaucga auuaaccgau     3720

gaaaugcuca uaaaacuaua cgagcuauuu uacaaaaaau uaaaagauac uguauacaac     3780

auacgauuau caacacaagu aaaaacacuu gaaucuggaa aagaaaaauu cgaaaagcuc     3840

agccuucucg acaaauguuu gguaauagaa gaaauucucc aucuauucca augucaguca     3900

gucgccgcaa aucuuucauc aaucggaggc gccgcaacag caggaauauu aguccuaaau     3960

aaugaaaucu cuagaugugg gaaaguagaa auaauaaauc aaagcauuac uggauucuac     4020

gaacaaguua uagauuuaaa aaauauuuga                                      4050


<210>  5342
<211>  4050
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5342
augcagaaau auuaucuagg ccuagacauc gguacaaaua guguugguug ggccguaacu       60

gaugaaaacu acaaccuaug uaaauuuggu ggcaaaagaa uguggggcau acgauuauuc      120

gauggagcac aaaccgcuga agaacgaaga augaauagaa gcaauagaag gcgacuuagu      180

aggcaaaaac aaagaauaga ccuuuuacaa gaauuauucg caaaagaaau agcuaaggua      240

gauccaacau ucuuuauuag acugaacgaa ucaagacugu auauggaaga caaaucaaca      300

ggaacaaaau uuccacuauu cgcugauccg ggauacacag auaaagaaua uuauaaagaa      360

uacccuacaa uauaccacuu acgccaagaa cuaauaaaaa accccgaauc acaugacaua      420

agacucguuu aucuggcgug ucaccacauu cucaaaaaua gaggacacuu ucuaauaaac      480

gguaguuucu cagaucugaa gaauuuaagu uucguaugcc gagaaauguu agacgccuuu      540

aacgaauuau cuaaauacca gcugaugaua ucagauaucg acgcauuaga aaaaauacuc      600

ucggacagaa aacaacaaaa aucugcaaaa guaaaagaac uagugcaaau auucgaaugc      660

guaccaagag aagauaaauc agaugcaaaa auuuguaaag aaauacuuuc uaauuuaugc      720

aaauuucuag ucggugcaaa aggaaacauu acccgacucu uugauacaga cuucgaugaa      780

auggaaacgu caucauuuuc auucggagau aguaaauaug aggauagccu acuuccuuca      840

uuagaagaaa auuacccaga uuacgcuaac guaauucaga aaauaaaggc aauguacgac      900

uggagcgucc uuguagaaau aauggcagga gagaacuauc uuucauuugc aaaaguaaac      960

gccuacaaaa agcacaaaga aaaucuuuca auacuuaagu cccugaugaa aaaauauugu     1020

acuccagaug ucuaccaacg auucuuuuau gaaacugacg aaaaaucuag cuacggaaac     1080

uacauuggcg ccauacauaa caacggacau ucacacacaa ugaaaaaaug cgacgaagaa     1140

acguucuaua agucacuauc caaaauauua gauaaacuag ucguucaaaa gacagaccaa     1200

gacacacuug agauacuaaa aaaagaagca aaaaaccaaa cauuacuccc uuuagccagg     1260

acaaaagcca auggaguaau uccaaaccaa auacacauac aagaacuuag agcaauccug     1320

gacaacgcca agacauacuu aucauucuua aaugaagccg acgaauacgg uacuauagcc     1380

gaaaaaauaa uacguaucgc aaaauucaag auuccuuauu augcuggccc acucagcacu     1440

cgacacaaag aacagggagc aaacacaugg auaguccgaa aacaggaagg ccgaaucuau     1500

ccauggaauu uugaagagau gguagaccua gagaggagca acaacgaauu uauaagaagg     1560

augacaaaua aauguacaua uuuaauagga gaagauguuc uccccaaaaa cucacuacua     1620

uacagcaaau ucaugguacu gaaugaacua aacaaucuaa aaauacgugg acacaagauc     1680

uccguagaau uaaaacaaga acuauauaaa gagcuuuucu guaagaaagc caaagucacc     1740

ggaaaagcac uuuuaguaua uuuaaaaaaa aaagacgcag agcuaacacu cgaggauauc     1800

ucaggauucg acgcagauuu caaaaguagc cucaguaucu acauugauuu uaaaaaucac     1860

auccuacgag aacaaauaga ugauggcaaa uaccaaucca uaguggaaaa uauuauaaaa     1920

uggaaaacga ucuuugaugu agacucaaaa augauagaga aauugauagc acgcgaguac     1980

ccaaacaugu uuuccucuga acaaaucaaa aaaaucugca gauuaaaaug uagaggcugg     2040

ggcaacuuuu caagcagauu ucuuaacgga auacaaggug aaaacacaga gacaaaagag     2100

uguuuuacca uaauugaagc cuuauggaaa acaaacuaua accuuaugca auuauuaucc     2160

aagcaauuca cauuuaaaga ugcuauagac ugcauaaacg ccgauaaagu uggcaaaaua     2220

gacaaaguga cauaugaaaa cacaguaaaa gacuuagugg uaucccccgc gaacaaaagg     2280

gcaauuuggc aaaccgucca aauaacagaa gagaucaaaa aaguaaugaa augcgaacca     2340

gaaaaaauau ucguagaaau ggcucgagga ggagaaaaag aaaaaaagag aacaauaagu     2400

cggaaggaac gacuauuaaa auuauacaug gauuauaaag acgaaaaucg agacuggaua     2460

aaagaaauug aauccagaga cgaacaagac uucaccaauc gaaaacuaua ucuauauuau     2520

acacaaauug guagguguau guacucagga gaggaaauaa gucuugaaga acucaugcaa     2580

aaaaauagua aaugggacaa agaccacauc uacccacaau ccaaaauaaa agacgacuca     2640

cuagauaacu uaguauuagc caggaaagac auaaauaaua acaaaucaaa uaaauuauua     2700

ccaacuggaa uucaagauaa aaugaaaaaa uauuggaaaa ugcuacuaaa auacgguuuu     2760

auuaguaaaa agaaauauga cagauugaug aggacagaag acuucagcga cgaggaacua     2820

uccggauuca uagcaagaca aauugucgag acuagacagu cgucaaaagc uguagcugaa     2880

uuacuagaac auuuaaauga acauacagaa auaguuuacg ucaaagcaga acucguauca     2940

gauuucagac augauaauaa caugcuuaaa agucgaagaa uaaaugacua ccaccacgcc     3000

aaagaugcau ucuuaaauau aguaguggga aacguauaca augcuaaauu cacagcaaac     3060

ccccggaacu ggauaaagaa aaaccgcaac acaaacuacu caauacgcag aguauucgac     3120

uauacaguaa aaagaggcuc aacaaucguc uggcaaggag uuaaagacgg cggacacucc     3180

auagauggca uuaagaaaac aauggcaaga aaugacauau uauacaccga aagaacauau     3240

ugugaacaag gcgaacuauu caacgccaca uuaauaaaaa aaggaaaucc gaacgcuaua     3300

ggacuaaaaa aaggacuaga cccaaugaaa uaugguggau acucaagucc gaaaacuagu     3360

gcauucgccu ucaucgaauu ugauggaaaa aaaggccgac aaaaucaccu aguggaaauc     3420

ccgguauaua uagcgaauau agcagcuaga caaccagaau auauaaagaa ucaccuggaa     3480

gaaaagaagg guuaucaaaa cgucauuguu aaaaaauauc ccauacaaaa aaacgcacuu     3540

auuaaacucg auggauaccc agcaagacua cgcggugcaa acgaaaaauc aauaacacua     3600

aagaacgcug uacagcucgu gcuuaagcca gaauaucacg agacgauaag aaacguugaa     3660

aaauacuugg aauacauaga uaaagacgag ucaguuagua aggcagcacg auuuagccac     3720

acagaacuag acaaacucua cgaugaacua ugcuuuaaag cagaaaauag uauauauaaa     3780

aaacgcccag caaaccaauc agcaacuuua aaaaauggac aaaaaacauu uuuagccaua     3840

acaaauuuaa aagaaaaagc acaagucauc aauaacauaa uaacgauguu uagaugcgau     3900

gccacaacaa cuacgaaucu aaaaacgaua gggggaucag cuaacgcagg aggaaugcaa     3960

gugaacaaaa acacacuaac aucacagaaa guaguauuag uacaccaaag uauuacaggc     4020

cucuucggaa cagaagagga acuguuguga                                      4050


<210>  5343
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5343
augaagaaac cuuacucaau cggccuagau auugguacca auucaguagg augggcggug       60

gucacagacg acuacaaagu cccggcgaag aaaaugacag uuuuaggaaa cacugacaaa      120

ucacauauua agaaaaaucu acuaggagca uuacuuuuug acucaggaaa uaccgcggcu      180

gacagaagac uaaaaagaac agcacggcga agauauacac guagaagaaa cagaauccua      240

uauuuacaag agauauucgc agaagagaug ucuaaaguag augacucauu cuuucaccga      300

uuagaagaua gcuuucuagu aacagaggau aaaaggggag aaaggcaccc uauuuuugga      360

aaucucgaag aagaaguuaa auaccaugaa aacuuuccua cuauauacca ccuaagacaa      420

uauuuagcag acaacccgga aaaaguagau uugcgacuug uauacuuagc gcuugcucac      480

auaauaaaau uccgcgguca uuuucuaaua gaaggaaaau uugacacaag aaauaacgac      540

guccaacgac uauuucaaga guuccuugcu guuuacgaua auacauucga aaacaguuca      600

cuacaagagc aaaacguuca aguagaagaa auauuaacag acaaaaucuc caaaucagcg      660

aagaaagaca ggguacuaaa acuuuuuccg aaugaaaaau caaauggacg auuugcagaa      720

uuucucaaac uuauaguagg caaucaagcg gauuucaaga aacauuucga acuggaagag      780

aaaacaccau uacaauuuuc aaaagacacc uaugaagaag aacuagaagu ccuacuagca      840

caaaucggag auaacuaugc ugaacuuuuc uuaagcgcca agaaacucua ugacagcaua      900

cuacuaucag ggaucuuaac uguaacugau gucucaacga aagcuccucu cucagcaucc      960

augauacaac gguauaacga acaccaaaug gaccuagccc aauuaaaaca auucaucaga     1020

caaaaauuau cagacaaaua caaugaaguu uucagcgacg ucucaaaaga cggcuacgcu     1080

ggcuauaucg auggcaaaac aaaccaagaa gccuucuaca aauaucuaaa aggauuauua     1140

aacaaaauag aagggagcgg auacuuuuua gacaaaauag aaagagaaga uuuucucaga     1200

aaacaaagaa cauucgauaa uggaucaauu ccacaccaaa uacacuuaca agaaaugaga     1260

gcaauaauac gaaggcaagc agaguucuau ccauucuuag cugacaacca ggauagaaua     1320

gagaaaaucc uuacuuuccg cauaccauac uauguagggc cauuagcgcg aggaaaaagu     1380

gauuucgcgu ggcucucgag gaaaucugcc gacaaaauua caccauggaa cuuugaugaa     1440

auaguagaua aagaaucauc agcagaagcc uucauuaacc gaaugacuaa cuaugaccua     1500

uauuuaccaa accaaaaagu acucccuaaa cacucccuuc ucuaugaaaa auucacagua     1560

uacaaugaac ucacaaaagu aaaauauaaa acagaacagg gcaaaacagc auucuuugac     1620

gccaacauga agcaagaaau auucgaugga guauuuaaag ucuaucgaaa agucacaaaa     1680

gacaaauuaa uggauuucuu agagaaagaa uuugacgagu ucagaauagu ggaucuuacu     1740

ggccuugaua aagaaaacaa agcauuuaac gcuucauacg ggacauacca cgauuuacga     1800

aaaauacuug acaaagauuu ucucgauaau agcaaaaaug agaaaaucuu agaagauaua     1860

guauuaacuc uaacacuauu ugaagaccgu gaaaugauaa gaaaaagauu ggaaaauuau     1920

ucagacuuac ucacgaaaga acaggucaaa aaguuagaaa gaagacacua cacaggaugg     1980

ggucgcuuau cggccgaacu uauccauggc auacgcaaca aagaaucccg caaaacaaua     2040

cucgauuacu uaauugacga uggcaauagu aacaggaacu ucaugcaacu aauaaacgac     2100

gaugcuuuaa guuuuaaaga agaaauugca aaagcacagg uaauagguga gacugauaau     2160

cuaaaccaag uaguaucaga cauagcagga agcccagcua ucaagaaagg uauuuuacaa     2220

ucacucaaaa uagucgacga acugguaaaa aucaugggac aucaaccaga aaacauagua     2280

gucgaaaugg ccagggaaaa ccaauucacc aaucaaggac gacgcaacuc ccaacaacgc     2340

uuaaaagggc uaaccgacag cauaaaagaa uuuggaucac aaaucuuaaa agaacaccca     2400

gucgaaaaca gccagcuaca aaacgacagg uuauucuuau acuauuuaca aaacggacga     2460

gauauguaua cuggugagga acuagacauc gacuaccuuu cccaauacga cauagaccac     2520

auaauaccac aagcauuuau caaagauaac aguauugaua auagaguuuu aacuagcagc     2580

aaagaaaaua gggguaaaag cgacgacgua ccaucugaag acguaguaaa ccgaaugcga     2640

ccauucugga auaaauuacu uucuuccggu cucauuucac agcgcaaaua uaacaaccug     2700

acuaagaaag aacuaacacc agaugauaaa gccggauuca ucaaaagaca auuaguagaa     2760

acaaggcaaa uaacaaaaca uguagcaaga auguuagaug aaagauucaa caaagaguuc     2820

gaugauaaca acaaaagaau aagacgagua aaaaucguaa cauuaaaauc uaaccuaguu     2880

uccucauuuc gaaaagaauu ugaacuauau aaaguacggg aaauaaauga uuaccaucau     2940

gcccacgacg cauaccucaa ugccguagua gugaaagcuc uauugguaaa auauccaaaa     3000

cucgaacccg aguucgucua uggcgaauau cccaaauaua acucauauag ggaaagaaaa     3060

acagcuaccc agaaaauguu cuucuauuca aauauaauga acauguucaa aucaaaagua     3120

aaacuagcgg acgaucaaau uguagaaaga cccaugaucg aaguaaacga cgaaacaggu     3180

gaaauagcuu gggacaaaac caaacauauc acaacuguaa agaaaguacu cagcuacccc     3240

caagucaaca uaguuaagaa agucgaagaa caaacaauag gacaaaacgg aggucucuuc     3300

gaugacaauc caaaaucacc ucuagaggua auaccaagca aacuuguccc uuuaaagaaa     3360

gcacuaaacc cagaaaaaua uggaggcuau caaaaaccaa ccacugccua cccuauacuu     3420

cuaauaguag acacuaaaca auuaauaccg auaaguguua uggauaagaa aagauucgaa     3480

cagaauccag uaaaauuuuu aaaagauaaa ggauaccaac aaauagagaa aaacaauuuc     3540

guaaaauuac caaaauauac ccuaguggau auuggcaacg gaauaaaaag acucugggcu     3600

agcagcaaag agguacauaa aggaaaccaa cuuguaguau ccaagaaauc gcaggaucua     3660

uuauaucaug cacaccauuu agacaaugau uacucaaacg aauaugugaa aaaccacuau     3720

caacaauuug auauauuauu caacgaaaua acaaguuucu cuaagaaaug caaacuagga     3780

aaggaacaca uccagaaaau cgaagaagca uacucaaaag aaagagauuc agccuccaua     3840

gaagaauuag cggauggauu cauaaaacua cuuggauuua cacaacuagg agcaaccuca     3900

ccauucaguu ucuuagguau caaacuaaau caaaaacaau acaccgguaa gaaagacuau     3960

uuauuacccu gcauggaagc caccuuaauc caucaaagua ucacuggguu auacgaaacc     4020

cgaauagacu uaucaaaauu aggaggagau uga                                  4053


<210>  5344
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5344
auggggaaga acuacuaccu uggguuagac auggggacau caucaguggg augggcagua       60

accgaugaaa aguacagaau uuuacgcgcc aaaggaaaag aucucugggg agugcgccua      120

uuuagugagg ccaaaacugc agcggaaaga cgaacacaca gaacaaguag aagaagacua      180

gacagagaaa aggcaaggau agccugccuc aaagaaaugu uugcagaaga aauuaauaaa      240

aucgauccag gauuuuauca aagauuaaau gacuccaaau acuuccuaga agauaaaaaa      300

gagcaucaac cguaugcaau auuugccgac acaggauaca cugacaaaga auacuacgac      360

gaauacccaa caauauucca cuuaagaaaa gcccuacuca acauagaaaa ugacaaaaaa      420

aaauacgaug uacgauuagu auaccuggca auacuuaaua uguucaaaca ucggggucac      480

uuucucaaug cuaaccuaga ugauaaaagu gggggaaacu ugaaagaaua cauagagaug      540

cuacaccaau caaugguuaa caucuuaaau auuaaucuaa aaaacgugga aauagagaaa      600

aucgaaaaaa uauuaaaauc uacccaauac ucaaauuccg aaagacuaga gaaccuguua      660

gaacuauacg aacugagcaa aaacaaaaac aaaauagaaa cggagaucuu caaauuuaua      720

uguggacuua aaggaacaau auccaaaauu uacguagaag auaaauucga agacgaauuu      780

gcuaaaauga cacuaagcuu ucgagacucc aauuuugacg aaaaaauaau agaaauagaa      840

gauaauuugg acgaugauaa auaugacaug uuccuucuaa uaaaacaaau acacgauugg      900

ucuguauuag caaauauaau gaacggagaa gaauauuuau ccguagcuag gguaaaacug      960

uaugauaaac acaaaaaaga ccuggagguu cuaaagaaau auuauaagca aaauucgaug     1020

gaagaguaua auaaaauguu uagacaaaug aaugacggaa acuauagcgc cuauguaggc     1080

uccguaauuu auaaagacaa cucaguacgc aggggcugca agagcaaaaa ggaagacuuu     1140

uacaaaaaua uacugaacac aaucaaaagu ugggaagauu gugaggcaaa agaguacaua     1200

accucagaaa uagacaaagg caauuuucua ccaaagcaaa uaaccgcauc caauggaguc     1260

auacccaauc aaguacacaa aaaagaacug aaaaaaauac uuaaaaacgc aucagaauac     1320

uuaaauuucc uuaacgaaaa agacgaaucc ggauacacaa uaagcgaaaa gauaguaaaa     1380

uuauucgaau uccaaauacc auauuacgua ggcccuauag cauacaacau cggaaacgac     1440

gaaucgaaac accgccauaa cauguggagu guuagaaagg aaaaaggacc uauauaccca     1500

uggaacuuug aacaaaaaau agauauaaaa aaaucuagug aaaaauucau uagaaaccua     1560

auaaaccauu guacauauuu gaacgacgaa gaaguauuac ccaaaaacag ccugcuauau     1620

gagaaauuua ugguucucaa cgaacuaaac aaauuaaaaa ucaauggcga gaaaaucucg     1680

guugaacuaa aacaaaauau auuuaaugac uuauucaaaa aagggaaaaa aguaaccaaa     1740

aagggacuca uuaaauaccu aaaagagcaa ggugaaauag auaauugcga agaaguagag     1800

aucucaggaa uagacggaga cuucacaaac aagcuaucaa acuacaaaaa auucgcggac     1860

auauuuggag uacaaagucu aaccuacgaa caaaccgaca ucgcagagaa uaucauaagg     1920

uauucaacua ucuacggaga cagccggaaa uucuuagaag aaagaauuag ggaagaguac     1980

ucaaauguac uagacgaaaa gcaaauuaaa agaauacuag gaaugaaauu uaaagacugg     2040

ggaagauuga gcaaagaguu acuagaacua ucaggcgucg auaaagaaac aggagaaauu     2100

gcaucgguga uaaguagaau guggaacgau aacuacaauu uaauggaauu aauagcuacu     2160

gagagguuca gcuacgcuua ugaaauagaa caacgaucca aaaaacuaga aaagacuauu     2220

ucaacuauag aauaugaaga uuuagacgaa cucuauauga gcacgccagu aaaaagaaug     2280

guauggcaaa caauauuagu acucaaagaa cucgaagaag uacuaggaug uccuccaaag     2340

agaaucuucg uagaaauggc gagagacgua aaugcaccaa aagacagaaa agacucacgu     2400

aaaaaaaaau uauuagaacu auauaaaaau uguaaaaaag aagaaggcga cuggucaaaa     2460

gaacuaaaug cacgagaaga aagcgaauuu aaaaguaaaa aauuauacuu auacuauaug     2520

caaaaaggac gguguaugua uaguggaaaa ccaaucgacu uaaagaaucu acuuaaugau     2580

aauuuauacg acaucgacca uauauacccc agacacuuug uuaaagauga uucaauagaa     2640

aauaacuuag ucuuaguaga gaaaaacuuc aacgcucaca aaucagauaa cuacccaaua     2700

gaaaacgaaa uaaggcagaa aaugcacucc auguggaaga ugcuuagaga cggcaauuuu     2760

aucacagagg aaaaauacag ucgccuuauc agaaaagagg aauucaccga ugaugagaaa     2820

gcaaaauuca uaucacgaca aauuguagaa acaagacaag gaacgaaggu cauaacagac     2880

cucuucgaac aaacauuccc agaaucugac auaguauacg uaaaagccgg aaauguaagc     2940

gaauucaggc auaaauuuaa gcuaauaaaa ugucgcaacg uaaacgacuu ccaccacgcc     3000

aaugacgcau acuuaaauau aguaguugga aacguauacu acacgaaauu uacaaaaagu     3060

gcacucaauu uuauaaaaga cuacaagaaa aauccagaaa agaacaaaua ccacauggau     3120

cacauguucc aauaccccgu aucuaggggc ggaguagacg cauggauaac aaaaggcgcc     3180

gaaagcauaa guaugguacg aaaaaugaug gcaaaaaaca ccccaauggu aacaagaaug     3240

aacuaugaaa agcacucggg ucaaaaaggc ggaauauccg accaaaauau cuguucagcc     3300

aaaaucgcca aaaaaaaugu auaccuacca auaaaaaugg gugacgaaaa acuaaaaaac     3360

guagagaaau augguggaua cggguccuua acagccaaau acuuuuuccu uguagaacau     3420

acaauaaaaa aaaaaagaau cagaacgauc gaagcauauc cuauguaucu agacgcacua     3480

aacaaagaaa acaaacuacu caauuacugu guacaagucc uugaauauga ugaaccgaaa     3540

auaauaaugg aaaaaauaaa aguuaagagu cuguucaaaa ucaacgggua uuacuacuac     3600

uuaaacgcaa aaucaguuaa uagaauaaua uggggaaacg caacacaacu auuucuggaa     3660

aaugauauaa acgaauacgu uagaaaaaua aguaaaaaca uagaauguau agauauuaau     3720

ucagaaaaua acaucaaagu auacgacaua uuuauagaaa aauauucaaa caaacuauuu     3780

aguaauaggc uaggcaaccu gaaagaggcg auaauccaaa accgcaacaa auuuauagaa     3840

cucaaaauca augaacaaau caauguaauc uuagaacucg uaagauauuc acaggacggu     3900

guguacagca ucaaucuuag ucaauuggga cucuccucaa cuuaugccau aaauaggacu     3960

ucaaaagaga uaaguaaaau gaaagaaugc aaacuaauca aucaaagcgu aacaggacua     4020

uaugaaaacg aaauagauuu auuaacugua uga                                  4053


<210>  5345
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5345
augcaacaau auuaucuggg cgucgauaug ggaagugcga gcguggguug ggcagugacu       60

gaugaaaaau accaauuagu cagaaagaaa ggaaaagauc ucuggggagu uagaacguuu      120

gauauagcuc aaacugcaga gguacgccgc gucagcagaa cgaacaggag acgacagaac      180

agaagaaaac aaagaaucca aauauuacaa gaacuauuag gggaagaagu auuaaaaaua      240

gacgccggau ucuuccaccg uaugaaagaa ucacgauaug uugccgaaga uaaaagaaca      300

cucgauggaa aacaaguaga acuuccauau gcucuuuucg uagaucaagg cuuuaccgac      360

aaagacuucu acaaacaguu uccgacaauc aaucacuuaa uagucuacuu aaugacgaca      420

ucggauacac cagauaucag auuaguauau cuugcacuuc acuauuauau gaaaaauaga      480

ggcaauuuuc uacacucugg ugacaucaau gauguaaaag acauacaaag uauuuuagaa      540

caauuggaaa acguucuaaa agaauacguu gacgauuggg agcucagucu caaagacaaa      600

guggacgcua uaaaagaaau uuacaauaaa gaccugggac guggugaaag gaaaaaggca      660

uucauaaaca cguuaggagu aaagacgaaa ucugcuaaag cauuuugcuc acucauaagc      720

ggcggaucaa ccaaccucgc agaauuauuu gacgacucag gacuaaagga gucagaauau      780

gcaaaaauug aguucgccaa cgcaaacuuu gaagauagug ucgaaggcau ucaagcacua      840

uuagaagaua gauuugcagu aauagaagca gcaaaacgac uuuacgacug gaaaaucuua      900

acugauauuc uaggagacaa ugcaucacug gcugaagcca gaguaaaauc auacgaaacu      960

caucacgaac aacucguuga guuaaagagc uuuauaaaga aauauuuaga cagaaaaaua     1020

uaccaagaca uauuuaucaa cccaaacauc gcgaauaacu aucccgcaua ugugggacac     1080

acaaaaauaa auggaaagaa acaagaacua gaaguaaaac gcgccaaaag aaacgacuuu     1140

uacgcauaua uaaagaaaca aguaaucgau ccgaucaaaa agaaaguauc ugauaaagca     1200

guucuagcac gauuagccga aauagagagc uuaauagaag uuaauaaaua ccuuccauug     1260

caaguaaacu cagacaacgg cgugauacca uaucaaauua aacuaaacga auuaagacga     1320

auauucaaua acuuagaaaa uagacuacca guguuaaaag aaaaucgaga uaaaauuauc     1380

aaaacauuuu ccuauagaau acccuacuac gucggaccuu uaaauggcgu uaaccguaac     1440

gguaaaucaa cgaacuggau gguucgaaaa gaaggggaag aaggaaaaau cuacccaugg     1500

aacuuugaag aaaaagucga ucuugaagca uccgcagaaa aauucaucag aagaaugaca     1560

aacaaaugua cauaccuagu aaacgaagac guacuaccca aauacagccu cuuauauagc     1620

aaauaccucg uccuauccga acuuaacaac cuacggcuug auggaaggcc auuagaagua     1680

ucgguaaaac aagaaauuua cgaaaaugua uuuaaacgga acagaaaagu aacacuuaag     1740

aaaaucaaaa auuaucuucu uaaagaaggu guuauaagcg aaaaagacga acuaagugga     1800

cuagcagaug acguaaaaag cucccucaca gccuaccacg auuuuaaaga aaaauuagga     1860

cacuuaaccc uaacggaaga ccaaauggag aaaaucauuc uaaacgucac uuuauucggc     1920

gacgauaaga aauuacucaa gaaacgacua gcagcauuau auccuaauau agaugaaaaa     1980

ucgcucuccc gaauggcaac auuuaacuau cgugauuggg guagacuauc uaagaaauuu     2040

cuaagcgaaa uaaccagcgu agaccaagaa accggagaac uacgaacuau uauccaaugu     2100

auguaugaaa cgcaaaacaa ccuuaugcaa cuacuaucag aaccuuacca uuucguagaa     2160

gcuauugaaa aagaaaaccc aaaaguagac cuagaaucaa uaucauacag aaucguaaau     2220

gacuuauaug uuucgccagc aguaaaaaga caaaucuggc aaacgcuguu aguaauuaaa     2280

gacaucaaac aaguaaugaa acaugacccc aaacgcauau uuauagaaau ggccagagaa     2340

aaacaggaau caaaaacaac aaaauccagg aaacaaguuc ugucugaagu auauaaaaau     2400

gcugaaaaau acaaaaacuu auucgaaaag uuaaauucac ugacggagga acaacuacgu     2460

ucaaagaaag uauauuuaua cuuuacccag cucggaaaau gcauguauac caacgaugca     2520

auagacuuug aaaauuuagu uagcgcaaau ucgaacuacg acauugacca uauauauccu     2580

caaucgaaaa ccauagacga uucauucaau aauuuaguuc uaguuaagaa agguauaaac     2640

aacgacaaau cugacagaua cccaauagac aaaaacauca gagacgacga aaaaguaaaa     2700

acucuuugga auacacuccu aucaaaagga uuaauaacua aagaaaaauu cgaaagauug     2760

auucgaucaa ccccuuucuc agaugaagaa uuagcgggau ucauagcccg ccagcuggua     2820

gaaacuagac aaucuacaaa agcaguagca gaaauacuau ccaauugguu cccggaaucu     2880

gagauaguau auucaaaagc caaacauaua acaaacuuua ggcaagauuu ugaaauacua     2940

aaaguaagag aacuaaacga uugccaccau gcgcacgaug cauaccuaaa uauuguagua     3000

ggaaacgcuu accauacaaa auucacaaau ucgccauacc gauucauuca aaauaaagcu     3060

aaccaagaau acaaccuaag aaaauuacua caaaaagcaa agaaaauuga auccaaugga     3120

guaauagcau ggauugguca aucugaaaac aaccccggca caauagccac aguaaagaaa     3180

guaauaagcc gcaauacugu auuaauaagu cguaugguaa aagagguuga cgggcaauua     3240

uucgaccaac aauuaaugaa gaaagggaaa ggucaaguac caauaaaauc auccgacgac     3300

agacucaucg auaucucaaa auacggaggu uacaauaaag ccaaaggagc auauuuugua     3360

uucauuaaau cuguuagaag aggaaaaacc aucaaaucuu ucgaauacau uccaguucau     3420

uuggcaaaga aauuugacug caacuuagaa cucuuaaaag aauaucuuga aucagaaaaa     3480

gacuuaaaua auguagaaau acuaaugccc aaaguaauga uaaacucguu auucaacuac     3540

aauggaucau uaauaagaau cccagggaga uaugacaaga aaucauuacu uauaaacguc     3600

gacguacccc uauuacucga aucgcaacau auaaaacaac ucaaagucau agaaaaauau     3660

auguauaaga aacguguauc caaaaacagc aacauacuac uaaccaaauu cgcaucagau     3720

caacuuaaag aucuugaugc ccuauuugac guccuaucau auaaauugaa cgaaaacauu     3780

uauaauguaa ucaaugacaa auaugauaaa cucguaaucu guagagauaa auucaucagu     3840

cuugacaccg aaguaaaaug cgaaaugaua uucgaacuac uacaucuauu ccaauguaau     3900

agccaacuug caaauaucac aaaaauugga gccacuucca aauucggauc gaucucaaug     3960

aguaaaaauc uaaaagaaaa ugauaaaaug ucaauuauac accaaucucc aaguggaaua     4020

uucgaacaug aaaucgaauu aacagcacuc uga                                  4053


<210>  5346
<211>  4053
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5346
augaaaagga uccucggcuu agauuugggc acagcaagua uaggcugggc cuuaguuaau       60

gaaucugaaa acgaaaaaga aaaaucagaa auaauaaaac uaggaguacg cguaaauccu      120

cuaacaacag acgaacaaac agacuuugaa aaaggacgac cauuaagcgu uaaugcagac      180

agaacauuaa aaagaggcgc gagaagaaau uuacaaagau ucaaacagag aaggaaaaac      240

cuaauagaaa uacucaucaa aaagggauuu auaaccaaua uaucgcuguu aacagaaaua      300

gguucgaaca ccacauauca aacucuagaa uugagagcga agaguguaaa agaaaagauu      360

gaaaaagaag aguucgcaag gguacuacua acaaucaaua aaaagcgcgg auauaaaagc      420

ucaagaaaag cuaaaaauga ugaagaaggc ucucuuauag auggaauguc gguugcaaaa      480

gaauuauaug acaauaacuu aacuguggga caauacguau accaacuacu cgaaaaagga      540

gggaaauaua uaccagauuu cuacagauca gaccuccaaa acgaauuuga ugucauuugg      600

aacuuucaaa aggaauuuua cccuaauaua cuaacuggag aauuauacaa gaaauuacaa      660

gggcaagguc aacaaaacuc ccgcaaacuc uucuuagcaa uucaucaaau auacaccgca      720

gaaaauaaag ggaaaagaga uacagucaag aaacaacacu acaaauggcg aaacgaugca      780

auaaacacac aacucuuaau cgaagaagua gcauacgucc uaguagaaau caacaacaac      840

uuaaacaaau caagcggcua ucucggugca aucuccgaca gaucgaaaga auuauacuuc      900

aacaaagaaa caguaggcga aaaucuauau aaacaaauac aagaaaaccc acaaacuucc      960

uuaaagaacc aaguuuucua cagacaagau uaccuagacg aguucgaaca aaucugggaa     1020

acacaagcaa aauuuuacuc cgaauuaacc aaagaacuaa aagaagaagu aagggaugua     1080

gucauuuucu accaaagaaa auuaaaaagc caaaaacacu uaauaagcga augugaauuu     1140

gagaaacacc auaaagcaau uccaaaauca uccccauuau uucaagaauu uaaaauuugg     1200

caaauacuaa acaacauuga auuuaaacau aagaaaacaa aagaacuaaa aauauuaagc     1260

gaagaauuaa aagaaacucu uuucgaagaa cucaacauaa gaggaaacuu uacaucugau     1320

gcugugcuua aauucguaaa acuagauaag aaagaauggg agcuaaauua caagaaaggu     1380

aucgaaggua auaggacaaa ugaagucuua uacaacgucu aucagaaaau ugcagaauau     1440

gaaggcuaug guuuugacug gucaaagaaa ucagcaguag agauuaaaga agaauuaaac     1500

accauauuca gcgaaauagg aauaaacuca aaaauacuag acuucaaccc agaaaucagc     1560

ggcaaugauu uugacaaaca agaaucauac caauuauggc aucuacugua uagcgcagaa     1620

gaagacggau ucuugaaaca aaaacuacaa gacaaauacg gguucaaauc agaauacgca     1680

aacaugcuag caaacgucuc uuuccaauca gacuacggau cuuuaagcgc aagggccauu     1740

aagaaaauac uuccuaauuu gaucgccggc cacgaguucu ccgaagcaug uucauuauca     1800

ggcuauaacc auagcaacuc auuaacuaaa gaagaacugg caaacagacc gcuaaaaggu     1860

aaauuagaac uauuaaagaa aaauucacuc agaaaccccg ugguggagaa aauacugaac     1920

cagaugguaa accuaguuaa ucaaguaauc gaaaguuaug gagaaaucga ugaagucagg     1980

auagaacucg caagggaacu uaagaaauca gcacaagaac gagcccuaau gacuacaaac     2040

auaaacgcaa gcaaaaccaa auaugaaucc uuuagaaaac uacuacaaaa agauuuuggc     2100

auuaaaaauc caacaagaaa cgacaucauc aaauacagau uauaugaaga auuaaaagca     2160

aacaaauauc auacuauaua cacgaauaaa uacauaccaa gagacaaacu auucucgaaa     2220

gaaaucgaca ucgaacacau aauaccaaaa gcaaaacugu uugacgauuc auucucuaau     2280

aaaacacuag cuuuucgaaa acccaaucag gacaaaggua acaugacagc uaucgauuau     2340

auucucagcu accaaucuaa agauauagac aacuauaaau caagaaucga aaacuuauuc     2400

aaaaacggga aaggcgauau aucaaaagcu aaacgaaaua aacuucucau ggaagaaaaa     2460

gacauaccag auggauucau agaccgggac uuaagaaauu cucaguacau agcuaagaaa     2520

gcaaaacaac uacuggaaga agucauacgc acaguaaaca cgaccacagg gucuguuaca     2580

aacaaauuac gagacgacug gggacuaauc aacguuaugc aagaauuaaa caugccaaaa     2640

uucaaagcaa uagacgaauc auuaauagaa cagauagaga agaaagacgg aaccuuuaaa     2700

acacguauaa aagacuggag caaaagaaac gaccauagac accacgcuau ggaugcgcua     2760

accguagccu ucacacgaua caaccauaua caauauuuaa auaaucuaaa ugccaaauca     2820

gacaagaaag gugcuauaua cgguauucaa gaacuagaaa cuuauguuaa cagcaaagga     2880

aaaagaaaau ucauaaaacc gaugcauaau uucagggaag aagcuaagaa acacauagaa     2940

gacauucuca uuaguuuuaa agccaaaaac aaaguaguga cuaaaaacau aaauaaaaua     3000

aaaagcaaag acggauucau aaagaaagua caauuaacgc caaggggaca acuucacaaa     3060

gaaaccguau auggcaaaau uaaugaagua guaguaaaag aagagaaaau aaacgcaaaa     3120

uucgaccucg agaaaauaaa uaaaguaaca aaacccauuu aucgaaacgc acuauuaaaa     3180

agauuacaag aauuuaacgg agaaccuaaa aaggcuuuua ccgguaaaaa caguuuaagc     3240

aaaaauccca uauaccucga uguugcacaa acaaaaaccc uaccagaauc cguaaaggua     3300

caauucuucg aagaaaauua caccaucaga aaagaaauaa gcccagaccu aaaaauagau     3360

aagaucauag auaaaggcau ccaaagaauc cuauuaaaac gauuagacga guucaacgga     3420

aagcccaaag aagcauuuuc uaaccucgaa aagaacccua uauggcuuaa uaaagaaaaa     3480

gguaucgcca uaaaaagagu uaaaauaaaa ggagucaaaa auguagaauc gcuccacaau     3540

aaacgugacc accuugguaa cgaaauacuu caaaaaggaa aauccgcacc aaacgacuau     3600

guaucaacug gaaauaacca ucacguagca auuuaucaag acaaaaaugg gaaacuccaa     3660

gaaaaagugg uuucauuuua ugaagcaguc gcaagaguua acaauaaauu accaauaauu     3720

gaucaagacu uuaauaaaga uuuaggaugg aaauuuuuau ucacgaugaa gcaaaacgaa     3780

auguuuguuu uuccaagcga agauuuuaaa ccacuagaaa cagaucucau ggauaagaaa     3840

aacaauagca aaauauccaa aaacuuauuc cgaguccaga aacuaggcaa caaagacuac     3900

acuuuccgac aucaccuaga aacuaaaaua gaagauauca agaaacuaag agacuucacc     3960

uacauaagaa aacgaaaucc cgaaucaauc aaaaacauaa uuaaagugag aaucaaucac     4020

uuagggaaca ucguacacgu aggcgaauac uga                                  4053


<210>  5347
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5347
auguccaaga aauacuauuu aggccuugau auagguacga acucaguugg cugggcagua       60

acugaugaaa acuacaaccu augcaaauau gcaggaaaaa ggaugugggg uauacgauug      120

uucgaaggag gagaaacagc ggaacaacga agaauaaguc ggucgaaccg cagaagauua      180

agccgaaaga aacaacgaau agaucugcua caagaacuau uugcagaaga aauagcgaaa      240

auggacccaa ccuucuuuau acgauuaaau gaaucacgac uucaucuaga agauaaauca      300

accggggaaa aauucccccu auuucgagac aaagacuaua ccgauauuca auauuacaaa      360

gaauauccga caauguauca ucuccgcaaa gaauuaauag aaaauagcaa agugcacgau      420

auaagauuag uauauuuagc cugccaccac auucugaaaa auagaggcca cuuuuuaaua      480

aacggcaguu ucagugacgu aaagaaacua aguucuguau gcgaaagcau gcucaauuca      540

uucaacggaa ucuccaaaua ccaaaucgag aucaacgaca uagaacgacu ggaagaaaua      600

cuauccgaca ggaagaaaca gaaaagcguc aaagcuaaag aauuaauaca aauauucgag      660

ugcggaccgu gcgaagauaa aacagaugag aaaacuugca aagcaauugu aucaaaucua      720

uguaaauggu uaacuggccu caaagguaau aucaauucaa uauuugauau gcaauuugaa      780

gaaauagaua ccaguucuuu cucauucaca gagccaaaau augaagacac aauaucugca      840

gcauuagaag aaaaauaccc ggauuaugcu gacguaaucc agaaaauaaa agcagucuac      900

gacuggucug ugcucguaga gauaauggca ggagaagauu aucuaaguuu ugcuaaaguc      960

aacgcauaua auaaacacaa agaaaaucug uguauacuaa aagaagugau aaggaaauau     1020

ugugauucag aaauauauca uaaauucuuu aacggauucg acgagaaaaa uggguaugga     1080

aacuauauag guucaaucua caaaaaugga cacucauacg caaugaagaa augcaguaca     1140

gaagauuucu auaaaacacu cgguaaaaua cucgauaaaa uacaacccga aaaagaagac     1200

gaaaaugucc uagaagaguu aaaaguugag gucaaaaauc aaacacuucu uccacuagca     1260

cgaacaacgg caaacggaac aauaccaaac caaguacaca uccaagaacu uagagcaauu     1320

cuggauaacg caaagaaaua ccucccauuu uuaaacgaag aagaugaaua cggaacaguu     1380

gcagagaaaa uaauaagcau agcaaaauuu cgcauuccau auuacguagg uccucuaagu     1440

acaagacaca aagaacaagg agccaauuca uggauuguuc gaaaacaaga agguagaauc     1500

uacccuugga acuucgaaga aaugguagac uuagaacaau ccaauaaaga guucauacaa     1560

cgaaugacua auaaauguac cuaucuaaua ggcgaggacg uccugccuaa aaacaguuua     1620

uuauauucca gauacauggu gcuuaacgaa uuaaacaacu uacgcaucag gggaaauaaa     1680

auaaguguaa aacuaaaaca agaacuguac aaagaacuau ucugcacaaa agcuaaagua     1740

acuggaaaag gacucuuuaa cuaccucaag aaagaagacg aagaauuagc acuagaagau     1800

aucagcgguu uugauaucga cuucaaaagu ucguuaacuu cuuaccuaga cuucaaaaag     1860

caaauacuag gagaagaaau agaaaaagau aaauauaaag guauaguaga aaauaucaua     1920

aaauggaaaa ccauuuauga ugacgacuca aaaaugauga agaaaaugau agaaagagaa     1980

uauccaaaug uauucaguaa agacaaaaua aagaaaauuu gcagauuuaa auacagcgga     2040

ugggguaacu uuagccuauc uuuucuaaac ggcauaagag gagccgaccg agagacagga     2100

gaaaguuuca caaucauaga agcauuaugg aaaacgaauu acaacuuaau gcaacuacug     2160

agcaaacaau uuacuuuucg agaagaaauc gaucuaauaa acgcggaaaa aguuggaaaa     2220

auuaauaaag uaagcuauga caauaccgua aaagacuuaa uaguuucccc ugccaacaaa     2280

agagcaauau ggcaaacagu acaaauaaca gaagaaauaa agaaagucau gaaaugugag     2340

ccagagcgca uauucauaga aauggcuaga ggaggagaaa aagagaagaa aagaacggua     2400

agucgcaaag cgagauuauu agaacuuuau gcagcaugua aagaugaugu aagagacugg     2460

acaaaagaga uagaagaucg cgaggaaagg gaauuuaauu cgaagaaacu auaucuauau     2520

uacacacaaa ugggaagaug cauguauucu ggagaggaaa uuaauauaga cgaacuuaug     2580

cagaaaaacu caaaauggga uauagaucau auauacccac aauccaaaau aaaagaugau     2640

ucauuagaca acaugguuuu agcgaagaaa guuauaaaca auaguaaaca aaacaaacug     2700

uuaaguucag acaucagaau caaaaugaag aaauacugga acgcauuaua uaaaggaaac     2760

uucauaucaa agaagaaaua cgaucgcuua acaagaacgg aagauuucac aaaugaagag     2820

uuaaguggau ucauagaacg acaaauagua gaaacaagac aaucaucaaa agcaguagcc     2880

gaacuccuua accgacuaua ugaacauagu gagauaguau auguaaaagc aggccucgua     2940

ucagacuuca gacaugacaa caaacuuuua aaaucaagaa gaauaaacga uuaucaucau     3000

gcuaaagacg ccuucuugaa cauaguaguc ggaaacguuu acaacgcaaa auucacaagc     3060

aauccuagaa aauggauaaa agaaaauagg ggcaccaacu auaguauaag aagagucuuu     3120

gacuacaacg uaaaaagggg uaacacagua guauggcaag ggguugaaga ugggggccac     3180

ucaauagacg aaauacgaaa gacaauggcc aggaaugaca uacuauacac ggaguauacc     3240

uauugcgaua aaggggaauu auucaaugcg acauuaauca agaaagguaa cgauaaagca     3300

auaaaccuca agaaagcacu agauagcguu aaauauggag gauacaauuc uccaaaaaca     3360

agcgcauucg ccuucauuga auuugacggc aaaaagaaag aaaggaaaaa ccacauagua     3420

gaaauuccag ucuacauagu uaauaucgca aaaagacaac cagacacaau aaaagaguac     3480

cuugaacaaa agaagggaua caaaaauguc guaguuaaga aauacccuau uaagaaaaac     3540

gcacuaauaa agaucgacgg cuaucuggcu agauuaagag gggcaaauga aacggacauc     3600

cuacuaaaga augcaguaca gcuaauucua aacgacaaau auuaugaaau aauaagacga     3660

guagagaaau accuagaaua uaauguaagg uaugaagcag aaucuauguu ugaaggcuuu     3720

gaccacgcag accuaaacag cuuauaugau gaauuaacga guaaauuaga gacaaguaua     3780

uacaagaaaa gaccugcaaa ccaacuaaau gcacuagaaa aggggaaaga gaaauuuaaa     3840

aacauucaaa ccuuaagaga aaaagcgcaa aucauaaaag aaaugcuaau aauguaccgu     3900

ugugacgcug cuacagucgc caaucuaaag gcgauuggag gauccgcaaa ugcuggaagu     3960

augaaaauca agaaaaauac auuaacaucc agaagucuag uacuggucaa ucaaaguaua     4020

acaggacucu ucgaaacaga agaagaacuc guuuga                               4056


<210>  5348
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5348
auguauaaga aauauggcau aggacuggau auaggcacau ccucaauugg auggagugua       60

guagaugaau acggacacau aauaagagua aaaggccaaa ccggucuagg aguuaggcua      120

uucagggaag ggcaaccugc agcagauaga cguacuuuuc guaccacccg cagacguuug      180

gguagaagac gauggagacu aagauuauua cgagaaauuu uugacccuua cauagccgcu      240

caagauccua auuucuuugc acgacaaaag gccucagcca uagcuccugg ugauucuaga      300

uuuaaagcug uguacagacu auucaaugac cagacugaua gacaauucua cgauaaguau      360

ccaacaguau accauuuacg acaaaaauua augacagaga aaaggcaauu ugacauacgc      420

gaaauuuauu uagcaguuca ucauauugua aaauauagag ggcacuuuuu aacaggcgga      480

gcugccaaga acuucaaacc aggaaaauua gaucuaaaca cauacuucgc aacuauaaac      540

gcgcaguuag cagccauauu uauggacuca caagucgaau uaccacaacu agauugggau      600

gcaguaguuu cucuccuaac uaacgaucag gaaaguccau cugaccguca aaaagcauua      660

acaaaaaugc ucguugcuca aggagaugcc acccagaaaa gauuuuacac cgaguucucc      720

aaagcacuac uaggccuaaa agcuaaauua uacgucuuaa caggauucga aggugacaag      780

acagaucaaa aagcauuaac gcuuucacua gaaacacuug acgaccacca agacgaugug      840

acacaacuac uuggugauga acaaagacag uuaguggaac uccuagcacg uguacaaucg      900

gcaauucaac uggcaaucau aauaccaaac ggcaaaacau uuucaggcca aauggugcaa      960

caauaucaaa cacauuacga ucagcuacga cugcucaagg acuaccgcga acaauuaaca     1020

gaucaaaaac accguagagc guuaaaacaa gccuaugcug acuacuuagc aaacgaucaa     1080

gaucaagacg cauuaguaaa ggccguugaa aaaguuuuaa aacagaacca accccuuucc     1140

acuguaggag uacgucuaca aacagcaaua gaugcuggug auuuucuccc uaaacaaaga     1200

acgaaggcaa acucaguaau accacaucag auacaucaac aagaacuaga ccgaauaauu     1260

gaagcacaaa gcguuuacua ucccugguua gcggaagaaa acccuguugc agcccauaga     1320

ucauacgcac ccuacaaacu ugacgagcua guugacuuuc gaguaccaua cuacguagga     1380

ccacuuguuc aaccacaagc ugauaaagcc gcuaaagaaa caaaauuugc auggaugaca     1440

cgaaaagagg cgcaauccac aaccccuaua acuccgugga auuucgaaca agaagucgau     1500

cguaaugcau ccgcaaacgc uuucauucaa aggaugaaaa caacagacac auauuuacuc     1560

ggggaagacg uucuuccgaa gaauagucua uuauaucaaa gauucgaggu auuaaaugaa     1620

cuaaacaacg uaagaguaga uggaaaauug uuaacuauag cagaaaagca acgccuauuu     1680

caggaucuuu uugucgaucg aucacaagcu agcguaacaa ucgcucaacu gcaagcuaau     1740

cuuauugccc acggcgcugc gaaagaccgc guagaaauca aaggguuagc cgacccaaaa     1800

agauucuuau cuucuuuaac cacuuaucac gauuacgcac agauuauacc cgaagcauua     1860

gagcagcccg acaaaagagc ggacauugag aaaauaauaa auuggaguac aauauucgaa     1920

gauacaucaa uauucaaaga aaaacuucaa gaaauagcau ggcucacacc ggaccaacgu     1980

aggcagcucu cacagaaaag guauagaggg uggggacaac uauccgacaa auuguuaaca     2040

caacuucgcg augcaacagg ccaaucagua auggauuuac uauggacaga accuaacaau     2100

uuuaugcaaa uuauacgacg uacugaauuu caacaagcaa uagcacgcgu caaucaaacu     2160

gaucuuaaac aacaagacuu agccacuaca auuaaugacc uuuacacauc accucaaaac     2220

aaaaaggcga uccgucaagu auuauuagua guagcagaca uacaaacugc caugcaugaa     2280

guggccccaa guuggcucuu uaucgaggcc gcaagaggac ccgaacagaa uccaaaaaga     2340

aaauuagcaa gacaacaaca acuacuagaa gcauacaaaa caucagcaaa agaaauuguc     2400

aaugaagcag uaaaagaaaa acuuuuauca gcaaucgaug aucaucaaaa auuugaagac     2460

cagguaguuc uauacuucca acaaaauggu uuagauaugu acacggguaa accauuagau     2520

uuugaacguu uaagcaaaua ccauauagau cauauaauac cucaaagcgu aguaaaggac     2580

gacagucuug auaauagagu acuaacaacu ccagaagaaa augaacgaaa aggagcaaga     2640

guaccaggcc cccuauacgc cucacaacaa ggacguugga gauuacuaca aagagcugga     2700

cuaauuagcc aaagaaaacu cgaucaucuu cuacuucgac cugaagaguu ggaucaacag     2760

gccacaggau uuguggcacg acagcuagua gaaacacgac aaguaaucaa acuaaucacu     2820

aacauucuau cgcaacaaua ugaucccgaa caaacuaaau uaguaucagu aaaagcuggc     2880

uuaucccauc aauuccgcac ccgguuaaau cucccgaaac uucgagaacu aaacgacuau     2940

caucaugccc acgaugcuua ccuagcagca agaauaggaa cguaucuccu acaaagauac     3000

ccaaaccuag aaagauuuuu cgucuacggc caauaucaga aaacuagauu cgaccuaaaa     3060

agacacuuua acuuuauagg agagauguua aaaggaaagc cagaggacgc aguaaucguu     3120

aaagccccuc acuccgaaac agucaucugg gauaaacaag augaaguaaa acaauuaaag     3180

aaaauaaugg aauauagaca ucuauuagua acucaugaag uauaugauaa ccacggagcc     3240

cuauucaauc aaaccauaca cccagccaaa aaccccaaaa auaggaaacu aaucccacga     3300

aaaacaacuc aaucaacaga acuauacgga ggauacuccg gagaaacuac aggauauuua     3360

gccaucgcac gaacgguuac gaaaaacaaa auaucauauc aaguacuaag aguaccgacc     3420

agacucguug gccgacuaca aagaucauca gaauccgcac aaagacaaau acuaacagac     3480

ugguucgcac caucauucgu uucuagaggg aagcaacaac cauucgaaau cguuuuaaga     3540

cauguguuca aaaaucagac uguaauagau gacauacaug aacaaucuca ucgauuuggu     3600

cuuagaucug auacguacua cugcaaucaa caacaauuag uacuaucguu agaacaacag     3660

agaaugauua uagaccaaaa auuaaacaac acaaacaaau uaaugguggu auacgaagcc     3720

gucugcgacc aaguagccaa guacuuucca uuauaccgaa ugaaucacuu uaaagaaucc     3780

uuauuagaag caggcccaaa auuagcugaa cuaccaauaa acaccguuca aaguaaaaac     3840

gguaagcuga aguuagguca aagauccguc cuccuacaaa caaugcaagg acuacacgcc     3900

aacccggaga caaaagaccu uagcauuaua ggaugcccca aauacuucgg uaaacuacag     3960

guaagcucug gcaucaaauu aaccgaaaac gcaguacuaa uacacgaauc ccccacuggc     4020

uuauuccaac ggcguguucg uuuaucagac cuuuga                               4056


<210>  5349
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5349
augaaaaucu uaggacuaga cuuagggaca aacaguauag gaugggcagu agugaacgag       60

gcagaaaacg aaagagaaag cucuagcaua auuagacuug guguacgcgu gaacccauua      120

acaguagaug aacaaaauaa cuucgaaaaa ggaaaaccaa uaacaacaaa ugcugacaga      180

acacuaaaaa gaucuaugcg cagaaaccuu caacgcuaua aacuacgacg ugcgaaccuu      240

guaaguuuau uaaaaacaca aggauggaua acagaugaga cucuucuaag cgaacaaggc      300

aauaacagua cuuuugaaac cuauagacuc agagcugaag cagccacaaa agagauaacg      360

uuagaacaau uugcacgagu cuuacuaaug cuaaauaaga aacgcgguua caaaucuuca      420

agaaaaauga aacacgauga ggacgguucg uucgucaaua acuuagauau cgcccgcgag      480

cucuacgaca guggucuaac cccgggacaa uaugcuuaca aucuucuuaa agaugguaag      540

aaauacaucc cugacuucua cagaagugac cuccaagagg aacuagauaa aaucugggcg      600

uaccaauaug aauaucauga aaauauucua acucacagcc uucgauccgc aaucauagau      660

caaagcaaaa caggcacuac aaaaauauuu uuaaaaacuc auggcaucuu cacagccgac      720

aucaaaggaa accgaaauga acgaaauaag caacuauuug aauugcguug cggagcucua      780

gaaagagcau uaacagcaga agaacuagcg acaguaauug cagagguuaa cggacaaaua      840

aauucaagca guggcuaucu cggagcuaua ucagaccgua guaaggcucu uuuuauaaac      900

caucaaacag uaggacaaua cuuaaugcaa cagcuagacc gcgacccaaa caaaucacuc      960

aaaaaucaac cauuuuacag gcaagacuau cuagaugagu ucgaacaacu augggaaaca     1020

caagcacacu accauccaga auuaacagca gccuuaaaac augaaaucag agacauaaua     1080

auauuuuauc aaaggagacu aaaaagucaa aaauggcucg uggcuaauug cccauuugaa     1140

cauagaaaag uauguccgaa gaguucucca cuguuucaac auuucaggau auggcaaguc     1200

cugaauaaug ugacugugac cguagauaau aaaaccuaug uccucaacga agaacagaaa     1260

aguauacuga guaaagaacu ccuguacaaa gagaaaauaa guaaaagaga ugcacuaaaa     1320

auguuauucg uaaaagcaaa gggagucgau augaauuucg cgucucuaga aggagacaga     1380

accaugucga accuacucaa ggcagcccaa gaaauaguga gcaucucagg acauggugau     1440

uacgacuucg gaaaaaugcc ugcggaugac auacauaaca ucauaagaag uguauuuggg     1500

gguuuaggau ucgcgacuga uugguugaau cucgauacag augcagacga ucucgaacaa     1560

gaaagucaaa ugagguugug gcauuuaaua uauucauuug aaggagacaa uucgacuacg     1620

ggggacacga agcuagugga aaggcucaug ucacuuuuga accuacccca agaauaugcu     1680

agauuauuug cuaacguaca uuuggaagac gauuauugcg ggauuagcag uaaagcucuu     1740

aaacgaauac ucccguuuau gaaagcaggc cauauuuauu cagaagcgug ugcacuagca     1800

ggauacaaac auagcgaaca aagccuuaca agggaagaaa auaaaaacag accuuuaaaa     1860

gaucaucuag aacaauuacc caaaaacagc cuaagaaauc cuguagugga aaagauacua     1920

aaucagaugg uaaauguagu caauagcaua auagacacau acggucgacc agacgaaaua     1980

cguauagaac uagcaagaga auuaaagaac aacgcgcuag gaagacaagc aauauucgaa     2040

gcuauaaaca ggaacacaaa agaaagcgag cgcauucagc aaauacuaaa gaaagaguuc     2100

ggauugacuc auguaucaag aaaugauaua auaagauaca gacuuuauca agaauuaucu     2160

ggaaauggau acaaaacucu auacuccaac aucuacauac cucaagaaaa acuuuucagc     2220

aaagaaauag auauagaaca cauaaucccc caagcaagac uuuuugauga uucauucagc     2280

aauaaaaccc uggaauauag agacguuaau auaaagaaau caaauacaac ggcaauagac     2340

uucguaagaa acgaauacgg ugaaaaugaa gucgaacaau auaagaaacg aguaaacgau     2400

uuacuuaaaa guggagcaau aucccaagca aaagcuaaga aauuaaaaau gacagaagcu     2460

gaaauaccua augauuucau agaaagagau uuaagaaaua cacaauacau uucacggaaa     2520

gcacaagaaa uguuacgaga cguagcuaga guggucacug cuaccacagg uaguguuaca     2580

gacagauuac gacaagacug gcagcuagua gacguaaugc aagaacuaaa uugggacaaa     2640

uacgaacguc aaggacugac aacgguuaua aacggucgag acggccaaca aauacgaagg     2700

auaacagauu ggacaaaaag aaaugaucau cgacaccacg ccauggacgc ccucacaaua     2760

gccuuuacca aaccagcaua cauaucauau cuaaaccauc uaaacgcaag gagcgaggau     2820

gacauaagga gcauugaaag acgauuucua gaaagagacg caaauggaca ccuaagauuu     2880

aucaguccaa ucgcccauaa uuuucggagu gaagcuaaaa gacaccuaga aaugauacuu     2940

guaagcauaa aagcuaaaaa uaaaguugug acaagaaaca auaauacaac aaaaaccaaa     3000

ggaggaaggc guaauagaau acaauuaacg ccaagaggcc aacuucacga ugcaacaguc     3060

uacggaucua uacuauuacc acauacaaaa acuauagccg uagacgguaa aaugacagcc     3120

gaaauaauag gacaaguaca aaauccaguc uuccgucagg cacuaaguca gcgauuagag     3180

gccuguggaa augauccaaa gaaagcauuc acagguaaaa acucacugga aaaagcucca     3240

guauggauag accaagcaca uacagaacau gucccagcag aaguaacaau ccaacgucug     3300

gaaccacaac auaccauacg gagagcagua aacaaagauc ugaauauaaa caaaguucua     3360

gacaaaggaa uccaacauau auuacaagca agacuagacg ccuacggagg agacgcagcc     3420

aaagcauuug caaaucuuga uaacaacccu auuuggcuaa accaggaaaa aggaauugca     3480

auuaaaagag uaacaacagc agagaauaua aguggagaac ccuugcacca caaacaugau     3540

aaagaaggga gaccuauacu agacacaaau gggaaaccaa ugcccucaga uuuuguaaga     3600

uuaaugaaca accaucacgu ugcaauauuu caagaugccg acgguaauuu acaagagcac     3660

auaguaucau uuuaugaagc gguagcuaga gugacggaac uaggauugcc cguaauagau     3720

aaagaauaca acaaagacaa aggauggcaa uuccuauuua cuuugaaaca aaaugaauau     3780

uucguuuuuc caaauccagc acaaggauuc aacccagacg aaauugaucu uacggaugaa     3840

agaaauuacu cgcggauaag cccaaaucua uacagggugc agaaaauguc aucaaaauau     3900

uauguauuca gacaucauuu ggaaaccacu guagaagaua agaaagaacu acaaggaaua     3960

acuuggaaaa gaauccaaag ccuaaauaac cugaaaggga uaguaaaagu acgaauagau     4020

cacauaggaa gaauuguuca aauaggagaa uauuga                               4056


<210>  5350
<211>  4056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5350
auguuuucac aacaaaauaa ucuauacugc gaaagccuau caaaacuaua uuauaauaua       60

acaaaaaauu uucucagcaa aaacgagguc ugcagaauuu augaaaucuu uuucuguuca      120

auauuaacau acuuccuaaa aaaguacaau cacgauauau ucaaaaaaag cucaaccaug      180

aaaaccaucc uuggccuaga ucuggggguc acaucaauag guugggcagu aauaguaacg      240

gacgacgaca acacacccau aaaaauacug gggaugggcu caagaauaau cccacuaagc      300

accgcugaua aagaagaguu ccaaaaaggu caagccauau ccaaaaacca aucccgcaca      360

auagcgagaa cgcaaagaaa aggguacaau cggaaacaau uaagaaagga agaacuaaaa      420

aagaucuuag gagaacuugg aaucacuccg aguaaagaau ugcuaaaccu accguccaua      480

gaauuaugga aauuacgagc ugaugccgcc aguggaaaug caccacuuuc agcagaacaa      540

cucggaagaa uauucuacau gcugaaccag aaaagaggau acaaaagugc gagaagugaa      600

gcaaaucaag acaagaaaga uaccgacuau guagccgcug ucaaaggaag acaccaacaa      660

cuaaaagaga aaggccaaac aauaggucag cacuucuaua cagaaaugcu aacauuagaa      720

gagaacagcg ggcacuucca aguaaaaaau aauguuuacc cgagagaagc auaccucgaa      780

gaauuuaaug caauaauagc ugcacaaaaa ccccagcaug ccuucuugac ggaugacgua      840

guugaccgac ugcgagauga aguaauauuc uaccaaagaa aacuaaaaag ccaaaaaggc      900

cuaguuagua uaugcgaauu ugaaggcuuu gaacgaaccu uuuuugacaa cgugaaacaa      960

aaagauaaaa caauauucac agguccgaaa guagcgccaa aaucgucgcc auuauuucaa     1020

cuuuguaaaa ucugggaaac cgugaacaau auuaaccuaa aggucaaaaa uacugacggc     1080

ucaaaaaaca aaugggccga uuacguuccc acuuuagaac aaaagcaaga aauuauugaa     1140

caccuauuua aaaacgcagg aaucucguua gaugccuuaa gaaaaauauu aggguucaau     1200

aaagacgaaa uauucguaaa uaaacaaaua gagaaggguc uacaaggaaa cacaacauau     1260

gccgaaauac acaaaauacu cggcgacagu aagcccgaau uguuaggauu caauauuaac     1320

auagaaccau caaaacauuc ugcucuacuu guugacaagc gcaccggaga aauacuagca     1380

gagaaggagg gacaacaagu aucugcagaa auagaaaaag agaauuuuua ccagcucugg     1440

cacacgauuu acucuauaaa agaccuagac gaaugugccg cugcccuaau aaaaagauuc     1500

aacaucgacc ccaacguagc agcucaguug gcgaaaaucg auuucucaaa aggagccuuc     1560

ggcaacaaau cuaauaaggc caugagaaag auauuaccgu acuuaaugca agguuacaau     1620

uacgcagacg caugcagucu agcggguuac aaucacucgg acucacaaac aaaaaaagaa     1680

aaagaagaaa acgugguaga cgauaaacua aaauugcuag aaaaaaacag cuuaagacaa     1740

ccaguagugg agaaaauaau caaccaaaug auacacguag ugaaugcaau aauagauaaa     1800

uacggcaaac ccgacgaaau aagaguagag uuagcgagag accuaaaaaa aaguaaagac     1860

gaacggaacg aagcugaauu acaaaaugcc ucaaacaaaa aguuaaacga agaagucgca     1920

aauagacuua aagaguuagg auuaccaguu accaaacggu ucguccaaaa auauaaauuu     1980

auauuucccg ugcgugauag aaaaauaaaa gaagcacaau uaguaaacca augcauuuau     2040

ugcggcgaaa cauucaacgu uacagaagca uuaucaggcg auaacuucga uguagaucau     2100

aucauaccca aaucaauguu auuugacgau agucaaacaa auaaaguauu aguccauagg     2160

aaauguaacg cuaaagacaa aaguaacaaa acagcuuaug auuucauagc ggccaaggga     2220

gaaaaugaac uugcaauaua ccuagacaga guagaugacu gguucaaacg aggggucuua     2280

aguuacggca aaaugcaacg uuugaaagua aguuacgagc aauacgcgga aagaaaacaa     2340

cuaaaaaaag aaacugaagc agauauaaaa cucugggaaa acuucauaga acgagauuua     2400

cgacaaacuc aauacaucgc uaaaaaauca aaagaucucc uuaagaaaau auguaacaac     2460

gucaccguaa cagaaggaaa gauaacugca caauuaagga accucugggg uugggaugau     2520

guacucauga aucuccaacu ucccaaauac aaagcacuug gccaaacaga aaucaaagaa     2580

uggacauccg aacacgggag acgucuacac aaaaaagaag aaauaacaaa cuggacuaaa     2640

agagaugacc aucgucauca cgcuauagac gcguuaguug uugccuguac aaaacaaggu     2700

uucauacaga gaauaaauac acuuaacucu ucagauacga aagaugcuau ggauagagaa     2760

auaaaagccg cuaaggaaaa auacaaugaa cuuuacgaca aaaaauuacu caccgaaaca     2820

aacgaacaag uaaagaauca cacagaaagg uucaacaaac uggaaacaua ccuuaaauca     2880

cagcaaccuc cacuuuucac uacaagauau guagaacaag aagcagacaa aauauucgua     2940

ucguucaaag cugguaaaaa agcagcaacg uacggaauca gaaagauaaa aaaagacggc     3000

aaaaaaguag uggugcaaga gaaaauagua guaccacgag gagcauuaca cgaacaaucu     3060

guauacggca aaauaaaaac ucaagaaaaa gauaaacccc uaaaauauuu auuugaaaau     3120

ccagaaacca uaguagacaa ucgaaucaaa auuuuaguaa aagaaagacu agacaaauuc     3180

gaaggcgaug ucaaaaaagc acucaccucu cuaaaaaaag agccguuauu cauugacaau     3240

gaaaaaaaca cccuacugga aaaagcagca ugcuacacag augccaccgu acugaaauac     3300

aaacuacaag cuuuaaaagc aaaccagguu aaugauauag uagacgaagg cguuaaacga     3360

auuguuaaag cgcgacuugc acaacacaag aacgaaaagg aagcauuuaa agauacaguc     3420

ugguucaauc aagagaaaag aauacccauu guauccguca ggguauucgc aagaccggau     3480

gcaagcaaau uagcaauaau uaaaacagac gaaaacaaca aaccuaucgg uuucgcacuu     3540

accggcaaua accaucacau agcaauauau aaagacaaca caggaaaauu aguacaacac     3600

auaugcacuu uuuggagcgc aguagaaaga aaaaaauucg gaguuccgac aguaguaaaa     3660

aacacaggua cucuaugguc ugaauuauua cauaaggauc uucccggaac guuccucgau     3720

aaauugccac aagacggguu agaacuagaa uauucauuac aacaaaauga aauguucguu     3780

cuuggauuac cgaaagaauc auuugaagac gcaauaaaga acaacgaccu accguuauua     3840

ucaaaacacu uauaucucgu augguccauc ucagacaacg auuacugguu cagacaucau     3900

uuggaaacca aaaacucaga acucaaaagc acaacaggag cuaaagaaag uaaacgauau     3960

uacagauuuc aaucaguagg aacauucacg aaggaaaauc caauuaaagu ucaauuaaau     4020

cacuuaggug agauaacaaa aauaggagaa ccauga                               4056


<210>  5351
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5351
augaaaaaaa gaaaccgaga ggaguacuac gugggacuug auauaggcac aaguaguguu       60

ggaugggcag uugcuacccc agacuacagg auaccaaagu ucagaggaca uaaaaugugg      120

gguagucguu uauuugcgga agguaaaagg gcagcagagc gaagacaauu uaggacaaau      180

agacgucgac ucgcuagaag acgagacaga cuacaaaucu uagaagaacu guuugcugag      240

gaaauggcga aagucgauaa cacuuuuuuu augcggcuaa aagaaucuaa auucuuccuu      300

gaagacaaaa ccgucaaaga aaaacacaua cuauuuucua cggacaaauu ugacgcugac      360

cgauuuuaca aagacaaccc aaccauauac cacguaagaa agagacuuau ggaagaagaa      420

ccacaagaca ucagagaauu auucuuggcu guacaucaua uauuaaaaua caggggacau      480

uuccuauucg caggaucauu agaacaaaac ggagcaagca uagaagaacu aguaagaacg      540

uuacuaggaa aacaaggacu cgccauauug gacgacacca uagacagcaa ugaaaugguu      600

aauauacuca aaaacacagg cgaaaccaaa ucagaucgaa aaaaggaugu acaagaauua      660

uggaacccau uaaaagauaa aguaaaaaaa gcacaagcag ccgaaauggc cgguuuagug      720

cuaggguuga aaagcgauau aagcaaacua uucgcaaacu cagauuauaa agaccucgau      780

uccucaauaa agaauauaaa cuuuagcuca ggagauuacg aagaaaaacg ugaugauuau      840

gcaaauagua uaggggauga cauaguauua cuggacacaa ugaaagcacu auaugacacu      900

uuaauuuuau cucaaauacu acaaggaaaa agaucaauaa gugcugccaa agucgaagau      960

uauaacacac auaaagaaca acuaagagca cuaaaagcga uauuaaaacc acauaaacgc     1020

uuauacaacg agauguucag ggaaaauaau aagaaagacc uaaguaauua uguagcauac     1080

aucucuaguu auagcgaagg aaaaagaguu aauagagaag gauuuuauaa auacguacaa     1140

gcuguauuaa agcaaguuga cgauucagau uccaaacagg caauacuuga agcaauucgc     1200

cuagaaacau uccuaccguu auuacgagua aaagauaaug cgguaauacc acaucaaauu     1260

caucaagccg aauuagaagc gauacuugaa acagcgagga aaaaauauaa uuucuuaaau     1320

gacguaucag augggguaag cguaaaagau aaaauaauuc aacuauuuac auuccgaauu     1380

ccuuauuacg uaggaccguu aaacccccac aguggggaga caguggcaga cagaaagcaa     1440

gggacaggau ggguaguaag aaaacaacga ggcaaaguau acccuuggaa uuucgcggac     1500

augguagacg uaaaagccag cgcagaagca uucauuacaa accuaacgaa caaaugcaca     1560

uauuuaauag gggaagaugu auuacccaag cgaagcuugu uauacaguga auuuauguua     1620

cuaaaugaac uaaauaugau caaauaugau ggccaugaau ugccaguagc cauaaaacaa     1680

uuguauauag aacaaguuuu caaacaagaa cauaaaaaac agacaaaagg uagaguacuu     1740

gauuuucuca aaagaaacgg auuaauaaca ggaucagcaa ccgcugucau uacaggaaug     1800

gaugaugcua uaaaaggaga uuuacaaagc uacagagaua ugguacgaau uuuuggcgcg     1860

ggcuuugauc auucaauggc cgaaaggaua auaagauggg uuacacuauu uggcgaagca     1920

aaagauauau uagagaauaa aauccgugaa guauacggga auucaauauc agacucccaa     1980

augcgagcca ucaaaaaacu aaaauacgca gauuggggca gacugagcca agcauuccuc     2040

aucgcaguua aaggaugcga caaaagcggc guagaaagga acauaauaac augcuuacgu     2100

gcaucaucac aaaaccuaau ggaaauacuc agcgaucaag guaccuauau aaaacagaua     2160

aaagcuugga aucaccaaca uaccacaaau augauggaua cacucgauua ucaacuugua     2220

gacgauuuau acgucgcucc uucaguaaag agaucaauau ggcagucucu cugcauagua     2280

gaagaauuag ucaaaauaau gggucaagcu cccaagaaaa ucuuuguuga ggugacuagg     2340

acaaaacaag cggaaaaaac aaaaaaagau ucuagaaaaa aaagguuggu agaauuauac     2400

acagccauag ggaaagaagg ugcagcaugg aaaaaagaaa uugaagcuca ucaagcugga     2460

gacuuuagaa gaaagaaauu auaccuauau uacacacaga ugggaagaug cauguauagc     2520

aacgagccaa uagcaaugga cgccuuauuc acaagugccu acgauauaga ucauauuuau     2580

ccacaaucau uaacaaaaga uggcaguugg gacaaucuag uacuuguaaa aucacaauua     2640

aacaaagaaa aaucagauca auauccccuu aggccagcaa uaagagagaa aaauaaagcu     2700

cauuggcaau accuaugcag agcuggguuc aucucagaaa aaaaauacga aagacuuaug     2760

agaacaacag aauuaacagc agaagaauua ucaggauuca ucgcuaggca acuagucgag     2820

accagucagg caacuaaagc aguuacaggg cuccuugaac gcauuuauac caacacagag     2880

auauguuuug ucaaagcuga aacgguuuca caauuuagac accaacauaa uuucauuaaa     2940

auacgcgaac ugaauaacua ucaccacgcc aaggaugcuu aucuaaacau cguaguaggu     3000

aacguuuauc acgaaaaauu uacaaaaaau ccaugggaau uuauugcgag aagcacagaa     3060

agaagaccgu auaaccugaa uaggauguac gacuuugauu uacuaaaaca aggacguguu     3120

auauggcaag ucccuaaaag uuuagaaacc guacaagcca ugaugaaaag caacgaugug     3180

agaauaacaa aaaaauuauu aguccaaaaa ggggagcugu augaugcaac cauaaccaaa     3240

gcacauaaag caaaggaggg aacuuacuuu ccacucaaag gagcugacgc aaggcuaaga     3300

gacgugcaua gauauggugg guacgaaaaa auaaaaauag ccuauuauuc uguguucagu     3360

uguagagaaa aagccucaaa cacaauucac acauuaauua uaccuauacc aguuuacaag     3420

gcaggacuau uacagacgga agcagacuug acugccuacg ccuggcaaac aaaacaaaaa     3480

acuaaaccaa uuaaagacau uuauucagca aucacuccca ucuauaaaaa acuauguaua     3540

aauacagugg uaaaaauaga cgguuuuaaa uacuuucuag gcggaaaaac aggagacuau     3600

auguggcuag auccugcaau uccuguagua cucgacgaac aagcugcagc cuaccucaaa     3660

gccauaucua aaguggggcg ugcggaugau guugaaaaca cggcacaagc caucgauuua     3720

auuacagcag aggcgaacga agccuuuuac gauguacuau uaaaaaagau ggaagcuaaa     3780

auauuugcca aggcguacca aacaauaguu gaaaaacuag caagugaaac cgcacgagaa     3840

agauucaggg aacucucaau aggcgcgcaa uguucguguc uauuagaaau acuaaauuua     3900

cuugcaaaca auaaaacaac auucaccuuc uucaaggaua aaaugggcgu aaccauguca     3960

agaagaaaac agccaaugga cuuaacuaaa cuugaagaau guaucgugua ugagacaagu     4020

aucacagguc uuuaugaaaa agcaguauca cuucuauga                            4059


<210>  5352
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5352
augggaaaac gauaccauau aggacucgau auaggcacau cgaguauagg gugggcagua       60

auggaugacg acuucaaaau aaugcgagua aaaggaaaaa aagguaucgg aguacgccuc      120

uuugaagaag gaaaaaccgc ugcagaaaga cgaggacaca gaaccacuag aagaagauac      180

agcagacgaa aguggagacu caacuuauua gaagaaauau ucgauccgga aaucgcaaaa      240

guagauccca cuuucuucgc caggcuaaaa gagagcaauu uaucaccuaa agaugaccga      300

aagcaauaca guggcaaucu aauauuuccg gaaaaggcaa aagaagaaau aagagaaugg      360

acagagaaau auccgaccau auaccauuua cgaaacgcgc uaaugacaga aaaucgacaa      420

uuugauauca gagaaguuuu ccuagcauua caucacaucg uaaaauaucg aggaaauuuc      480

cucuucaaug gcaauuucca aacaggcgaa guaaaccuca gcgaccguuu cgacgacauu      540

aacagcgcau uuauaucagc augcggagaa gaagaagccc cccaaauccc aucugaguua      600

uacaaugaua uucaagaauu acucuuugau aaaaauacca gaaaauuaga caaccaaaaa      660

caauuggcaa caaaacucuc acaagauaac gauaaagauu ggucaacaca auuuaguaaa      720

gcgcucuucg gcuacaagac uaaauucaaa gucguucuuc aaaguaacga agaucuugac      780

gacuuucaac uagacucaga cgacgcagau gauaaaauag caucaaucag cgaacaaaua      840

gacgacgaca gggguucaau cuuagauaau auaaaaauau uauauucacg gauaacucuc      900

aacgaaauag ucccagaugg uaagacucua agccaaagua ugaucgaaaa auacaacuug      960

cacagagacc accuaaaaga gaucaaggau cagcuaaaac auacagacaa cugggucaaa     1020

uuugaugacc ucuauuccca guauguaaac ggaaaacuag aaagugaccg ccauaagaaa     1080

uuaucaucag augacuucua caaaaaagua cuaaccuuau uugacgguga acuaaaaaca     1140

agagucaaag aguggcaaga gaaaggcgaa uuucucccaa aacaaaggac cggaucaaac     1200

ggagugaucc cacaucaauu acaccaaaau gaacuugauc aaauaauuga aaaucaaagu     1260

aaauacuacc cauggcuagc uacucuaaac ccaaaugaaa aaagacagag agucgcaaaa     1320

uauaaacuua gugaacuugu ugcauucagg auaccuuacu auguaggccc aauggugacc     1380

aaagaccaau cagacuucgc uuggauggua aggaaaucag acgaaaaaga aauuacucca     1440

uggaauuuug aucagguagu agacagagac aaaagugccg aggaauuuau caauagaaug     1500

acaacgaaag auacauaccu cauaggugaa gacguccuuc cagcaaaauc acuccucuau     1560

gaaaaauuca cagucuuaaa ugaacugaac aacauacgua uaaauggcaa accccuaacc     1620

accgacgaaa aacaccaagu auacagggac cuguuccaga acgccaaaag cauaucaaca     1680

aaaaaauuuc acaauuauuu aguauguaaa cuaaacaaac ccaaccaucu aaauauaaca     1740

ggauucucug aucugacuaa auucaacucu ucccuagauu cauacauuga cuugaagaaa     1800

auauucgcag accaaauaga cgaucaaagu cuucaggaug acuucgaaaa aaugauacga     1860

uggaguaccg uauucgaaga uaaacaaaua uuugaaagaa aauuaucaga aaucaacugg     1920

uugacugacc aagaaagauc agaaguagcu aaauuaagau acagagggug gggcagacua     1980

ucaaaaagac uauuagcggg aauacaugac gagaaugggc agcgaauuau ugacgcgcua     2040

uggaauucac caaaaaacuu uaugcaaauu caagccgagg uugcuuucaa caaacaaauc     2100

aacgaccuua acacaaaaga acugucagau aacaacgucg aagacgucuu agcagacgca     2160

uauacaaguc cccaaaauaa aaaagcaaua agacaaguuc uacuugucgu agaagacaua     2220

cagaaggcca ugcauggaga ugccccaaca ucaaucagcc uagaauuugc ccgggaagaa     2280

caagaaagua gcagaacacg aagcaggaga gcagccuuaa gcgcaaccua uaggcaaaua     2340

agugaugaaa uagucucugc aacaguuaaa ggagaauuag gaaaagcuac gaguauaagc     2400

gacagacuau acuuauacuu cacacaagga gguagggaua uguacacagg cgaaccgauc     2460

aacauagacg aaauaagucg uggauacgaa auagaccaua uacuaccaca auccuuuaua     2520

aaggaugauu cgcuagauaa ucgcguauua guaaaaggag ccauuaacaa uggaaaauca     2580

gcaaacuacg uaauggauca aucgugggcc ccagauagac uagcauggug gaaaaaauua     2640

cucgacaauc gauuaauuuc cagaaaaaaa uaccaaaguu uaacauuaac ccccggaaaa     2700

auaaauaagu acucucaaaa cggcuucaua aauagacaau uaguagaaac aagacaagua     2760

aucaagcucg uagccaauau ucuaaaugac caauauucaa acgaugauac aaaaauaauc     2820

guuguaaaag cagcauuaaa cucacaauua aggggcuuuu uagaacucuu uaaaaauaga     2880

aacauaaaug acuaccauca uggaauggac gcguaccuaa gcacauuuau agguaauuac     2940

cuuuauaaaa aauaucccaa acuaaggagu uacuucguuu acggagaauu caaaaaguuu     3000

uccggcaaac agaauuuaaa gucauucaau uuuuuacacg auuuagaaga cgauaguaua     3060

aauauaauaa gcaacgauga uggagaaaua guugguaacc aucacgaaau gaucgaagaa     3120

cuagagaaag ccuaucaaua caaauauaug cuagugucaa gagaagucuu cgaaaaaaca     3180

ggagaucugu ucaacgugac aguuuacagc cgaaaagcca aaaaaacccu aauaccgaua     3240

aaacaagaua aaccaauaaa uaucuacggc ggauauucag ggaacaauga cgcauacaug     3300

gccauaguaa aacucccagg uaaaaaagga aaaccagaca ccuauaaaau cguaggcaua     3360

ccacucagau uugccggaga acuugaacga gcaaaacaac uaggugaguc agcauauaga     3420

gagaaauuaa gugcaguaau aacaccccaa uucacaaaag agaaaaaaga uaggaaaaca     3480

ggugaaauaa caaaaguagu cgaugauuuu gaaguuguac uagauagagu accauaccga     3540

caacuauuua aagauucaga caagugguuu auguuaggau ccgcugcaua ccaauacaac     3600

gcaaagcaau uaguauuaaa caaugaugac gugaaguuac uaaacgaaca aggcagaaag     3660

aaauuagaac uuccaaacga aaaacucuca ucagcacuga acgacguauu ugaccacaua     3720

auacaaauaa ugaacucuaa guucgggcua uaugauaaaa acggauucag aacuaaguua     3780

acagaggcag caaguauauu uuacgauuua ccgcuuagag cagaguauaa uggaaauaaa     3840

aaaguaacag acggcaaacu agaaguacua gacaauauac uaauuggauu acacgcugac     3900

ccaucauucg gaaacuuaaa acaauuaaac auguccacac cauucggaca gaugcaaaca     3960

aaaggaggag ugguccuaaa cggaaauuca acccuuauau accaaagucc aacagguuua     4020

uucgagagaa gagucaagcu uaaagaccua ucuaaauga                            4059


<210>  5353
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5353
augaaaaaaa uccuuggauu agauuuagga acaacaucaa uaggaugggc cuucauaaau       60

gaaggcgaaa acgacaacga aaccaguagu auaauaaacg caggaguacg caucgugccc      120

uuaacaaccg acgaagaggc agauuucaaa aaagggaaua cuaucucuau aaacgcagac      180

agaacacuaa agcgcggagc aagaagaggc cuucagcggu uuaaacaaag aagagaugca      240

uuacuacaaa guuucagggg uauaaacuuu auaucaucga auuacguuua cgcagaaacu      300

ggaaaacaaa caacauuuuc aaguuacaaa cuaagagcaa agagcgcaac cgaacuuguu      360

acuaaagaag aauuagucca aguacucuua augcuuaaua aaaaaagagg auauaaaucg      420

ucaagaaaag caaaaacugc ugaagaggga gacgcaauag auggaaugaa aauugccaag      480

gagauauucg aaaacaaccu aacaccugga caauggguau auaacaguuu aucaauagga      540

gggaaauuca uucccgacuu cuaccgauca gaccuacaaa aagagguaga aaaaauagua      600

agauuccaaa aucaguuuca cccagaacaa augaacgaaa aauuauugga aaacauacaa      660

ggaaaagcaa gaacugucac uucccaauac uuuagucgaa uucuaaacau cccauuagcc      720

gaaaauaagg gcacucguga agaaaccaaa cuccaacauu acaaauggag aaacgaagcu      780

cuaaccgaag aacuagacuu aaaaguauua gccuuuauua ucacagaaau aaacaaucaa      840

auaaaccaau caaaugguua cuuaggagcu auuagcgaca gauccaaaga gcucuauuuc      900

aaccaagaaa cagucggcca auuccaguau aaucagcuga aggaaaaugc acauacaaaa      960

cuaaaaaauc agguuuucua ccgacaagau uaccuagaug aguuugaaaa aauaugggaa     1020

acacaaucaa aauucuaccc ugaauuaaau gacgaauuaa aaucggaagu cagagacauc     1080

acaauauuuu accaacgaaa acuaaaaucu cagaaacacc ucgucucauu cugcgaauuc     1140

gaaaaaggac acaaagcaau ucccaaauca aguccacucu uccaagaauu cagaauaugg     1200

caaaacauaa acaacauagu cgucaaaaac gagaccacaa aagaaccauu ucuacucgga     1260

gaagauuuga aaaagguaau agcccaagag cuaaguuuca aagagucaau gaccgauagc     1320

caacuauuaa cauuuugcga ccuuaagaaa ggaacacaca cagugaauuu uaaaaagaua     1380

gaaggaaacc gaaccaacac cucauuauuu aaagcuuucg aaaaaauacu gguauuagaa     1440

ggauaugacg aacucgaucu aucaaaacua gacgcgagcg acauaaaaag cauuauaaaa     1500

ucagccuucu cggaccuaga aauaucaaca gcaauuuuag acuuugaccc gacaauacaa     1560

ggaaaugacu augacaaaca aaguuacuac cagcuguggc accugcuaua uaguagugaa     1620

gaagaugauu uccuaaagaa aucucugaug gauaaguuug gcuucaaaga aucccaccua     1680

ccauuucuuu ugaacauaaa cuuacaagcc gauuacgggu ccuuauccgc gaaagcaauu     1740

aaaaaagucc uaccccauuu aauggauggu cacauauaug auaaagccug cacacuugua     1800

ggauauaacc acaguaguuc ucucaccaca gaucaaaaca augagagagu acuaaaagau     1860

acucuugaau ugcuuaaaaa aaacagucuc agaaaucccg uaguagaaaa gauacuaaau     1920

caaaugauca acgucauaaa cgcgaucaua aaagaucccg caauggguaa accggaugaa     1980

auaagaguag aacuugcaag agaacugaag aauaacaacg aacaacgauc ggaaaugaca     2040

aaagcaauaa acaaaucaac gacagaacac gaacagaucc gaaaguuauu acacacagaa     2100

uuuggaauac auagaguuac aaggaacgau auaauacgau acaaguuaug gaaagaaugu     2160

gaugggauuu cucuauacac gggaaagccc auagagccca gcaaacuauu cacgaaagac     2220

uaugauauug aacacauaau accaaaaagc agauuauucg acgacuccuu uaguaauaaa     2280

acuauaugcg aaagacaacu caacaucgac aaaucaaaua aaacggccua uagcuuuuuc     2340

caagaaaaac uaagcgccga agaauuugac caauucgaaa aaagagucaa aucacuuuuc     2400

ggaaaaauaa gcagaaccaa acaaaauaaa cucuuaaugg ccaacaacga gaucccugag     2460

ggauuuauag auagacaacu ccgagaaacc caauauauag caaagaaagc uaaggagaua     2520

cuauuagaag uaucacgaaa cgugacagca acuaucggau cuguaacaga caaacuuaga     2580

gacgauuggg aacucguaga uguuaugaag gaauuaaacu gggaaaaaua cgacaagcuc     2640

ggacuaacau acauagaaga aggaaaaaac ggcgaaaggc uauauaaaau aaaagacugg     2700

acaaaaagaa acgaccauag acaucaugcu auggacgcua uaacaguagc uuucacaaaa     2760

ccugcauaca uccaguaucu uaacaacuua aacgcccgaa ccaauacaga uaaaaaagga     2820

aauuccauaa ucggaauaga aaauaaauac cuaaaaagag auaaaaacaa uaaacuacgc     2880

uuuauagaac caauaccaaa uuucagagaa guagcaaaaa aacaacucga aagcauauua     2940

aucucuuaua aagccaaaaa uaaaguagug acaaagaaua aaaaugucac aaaaaaauca     3000

aaugguacaa aucaaaaaau ccaacuuacu ccaaggggac aauuacauaa ggaaacggua     3060

uauggcaagc uacaacaaua cguaacaaaa gaagaaaaaa uaaacgccaa uuucacacca     3120

gaauacguaa caaaaguugc gaaaaaagaa uaccgagaag cacuacucaa aagacuuauc     3180

gagaacgaaa augaucccaa gaaagcauuc acaggaaaaa acgcacuauc uaaaaacccu     3240

auuuacauaa gccuagaaaa uaacauauua guaccagaaa aaguaaaaau agucugguua     3300

aaauccgauu acaccauaag aaaagacaua accccagauu uaaaaaucga uaaagucaua     3360

gauaucggac uaaaaacaau acuacagaau cgguuaaacg aguuuaaugg agauacaaaa     3420

aaagcauuug ucaacuuaga ugaaaacccc auuuggcaaa acaaagaaaa aggaauagca     3480

aucaaacgcg uaacaauauc aggaguaucc aaugcacaag cauuacacac aaaaaaggau     3540

caccucggaa augaaauauu agaugaaaac ggacaacccc uaccaguaga cuuugucagu     3600

acaggaaaua aucaccacgu agccauauau agagaugaaa aaggaaaccu acaagaagaa     3660

gucguaaguu ucuuugaggc aguaacucgu aacaaucuag ggcuaucaau aauaaacaaa     3720

aaccaugaaa aaggauggga auucuuauuc ucaaugaaac aaaaugaauu uuucauauuc     3780

ccuacagacg gauuuaaccc acaugaaaua gacuuacuua acacagaaaa uuaucaucuc     3840

aucucaagaa auauguuccg aguccaaaaa auugcgacua aaaacuauau guuuaaucau     3900

caucucgaaa ccaaaucagu agacggggaa augcuuaaaa caaagaaaca acucucaaaa     3960

aucucauacc acuucaucca aacaccaagc uaccuuaacu acauaauaaa gauccguaua     4020

aaccaucucg gacagauagu gcaauugggu gaguacuga                            4059


<210>  5354
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5354
augaaacaag auuauuauau aggauuagac auggguacgg aaaguguagg augggcggug       60

accgacguug auuacaauuu aaucaagaaa agaggucaag acuauugggg uguauauuua      120

uucgacgaag cacaaacggc acaaggacgg agggcauuca gaacuaauag aagacgacuu      180

gcacgaguac gucaucgauu aaacuuacuc caagaacuau ucgauagcga gauaucggca      240

aaagauuuug gauucuucua cagguuagaa aauucaaaau acuucgacga agauaaaccc      300

gaaauugcga ggggaaaauu cuuacuauuu aacgauaaag acuucgguga caaagaauau      360

uacaaaaacu acccuacaau auuucauuua agaaacgcau ugagaacaca agaaauaaaa      420

gacguucgau uauuauauuu ggcgguacau cacauauuga aaaacagagg acacuuucua      480

uuuggggaac aagacuuuaa ugugaaugac gcgagugaug uaucagggga auuugagaaa      540

auaaacagau uccuaagcga aaacgacggg gcuggauucg aucgagagaa aauaccagaa      600

auauucgaaa cacucugcaa ugaaaaccua gccaaacgug aaagaaaaga gaaaauacua      660

gaauuagcag ggaacagcaa aaauaaacua uuaggugaag caguaaacgc uguuguagga      720

cuaaauuuuu cgcuauccaa aauguucgga gaagaccgcg auuuccaagg agaaaacaaa      780

augaguuuua cagauaguag uuucgaagaa aaacuagcua aagcuagaau ggcacuagau      840

gaugaugaag cagacuuccu ugaaacauug aagaaagugc aaagcguagc uucauuacau      900

aacauauuac acgguaaaga auuuaucagu gaagcuaaaa ccgaagcaua cgaaacgcac      960

agaagagacc uaucugaacu caagaaauau guuagagaaa auuguccaga aaaauaugcu     1020

uugguauuua gacgacaggu caagaaaggu aagaaaaaua cuucaggggc ugacgaaaac     1080

gaaaaagacg auaagaaagu aaauaacuac gcagcauaca uugggaugga caaaggaaaa     1140

gguuucgaaa aaugcaagaa agaagacuuc uacgcauucc uaaagaaaga aauaggaauu     1200

aaugaugaaa guuuacuagc caaaauagac gaagguauau uuuuacccaa acaaauuuca     1260

gcggaaaacu gugauauucc auaucaagua caccuagccg aauuaaaagc gauucucgca     1320

aacgcagaaa acuauuuucc cuucuuaaaa caaaaagaug aaaauugcga aaugacagua     1380

ucggagaaaa uaauaucauu aaugaaauuc aggguaccau auuauguagg accuuucaca     1440

gacaaagaaa caagauuuuc uuggaugauc aagaaagaag guuuaucaac acagaaaaua     1500

acgccgugga auuucgauga uguaguugau cgagaugccu ccgagaagaa auucauagaa     1560

aggaugacca acuucuguac auaccuaacg ggugaaaaag uacuaccugc aagcucauuu     1620

uuauacagcg aguacgcauu cuuaaacgaa cucaauaacc uuaaaguaaa cggaacuaaa     1680

gacuuaaggg ucaaaaacau uuuauuugac uuugcaaaaa caaacaagaa auuaacauug     1740

aagaaauguu gcgaacaacu gguuaaaaau ggacuacuac cagaagguag uaaagcaaga     1800

gauguauuua guggaaccga cggcgauuuu aaaaauaguu uaagcucaua uauagauuuc     1860

aaaagaauaa uuggugauaa agcugacuua uacagagaua ugugcgagaa aauaauuuua     1920

uggauaaccg uaauuaguga caaagaaagg cuagucaaaa ucaucaaaga uaaauacgga     1980

acgguucuau cugaugaaga aauaaaagga uuaaaagcac uaaacuacac uaaauggggc     2040

cgacuuucag aaaaacuauu agaugggaua uucgaaaccg acucagaagg agaaguagga     2100

aacguuucua uuauagagaa aaugagaacc gauacggaaa auuuuaugga auuacuaucu     2160

aauaaacaca acuauaaaag agcaauagaa cuggcaaauu cggaaaguau aguagacgag     2220

agagugacau accgagcggu agaagaauua uacuguuccc cagcuguuaa aagauccaua     2280

uggucagcac uaaaaauagu acgugaaauu guuaaaguac aaggacauag cccgaagaaa     2340

auauucguag agacgacuag agaaaaaccc gaugugacac gaaaagguca aaggacaguu     2400

ucuagaaaac aacaauuauu agacaaauac agagagaauu ugcgcaacga gacagaacua     2460

aucaagaaaa uugaagaaac agaagacucu aaauucaaua gugauaaacu auucuuuuac     2520

uuuacccaaa ugggaaaaga ugcauacacu ggagagaaaa uagaguucga ugacguauuc     2580

aaaacugaaa aauacgacau cgaccacaua uacccaagau caaaaauaaa agaugauagu     2640

cucgacaauc guguguuagu cagaaggaaa auaaaucaga gcguaaagaa agauucauau     2700

ccacuaucag ccgaaauucg caaucguaug gaaggcuuau ggagacaacu gagagacaag     2760

aaauuaauua gugacgagaa auuuuacaga cuuacaaggu caacuccauu aacagaaaaa     2820

gaaugcggug auuuuauuaa ugcucaauug auagcaacaa gucaaucggc aaaagcagca     2880

auccagcucu uaaaaagaau guuaccagaa ucagaaauag uauacaguaa agcaggaaac     2940

gucaacgagu ucaagaaaag auuagaauuu guaaaaauac gagaauuaaa ugacuuacac     3000

caugcaaaag augcauacuu uaauauagug guaggcaacg uuuucaacac gaaguuuaau     3060

cacaacccag cagucuuuca aagaaguaac ggaacaggaa auuaugauuu ugaaagacua     3120

uaugacuucg auauaacaaa ugccuggaag aagggggaug aaaaccgaau cagaaguguc     3180

gcagagaaga auacaugccg gguagucaga uucucaaaaa cggaaaaagg ccaauuuuau     3240

gaugccacaa uuuacccagc cggcaaaaac gauaaacucg cgccuguaaa aggaaaagca     3300

ccguuaaaug acacuucaaa auauggagga uacgauaaag agacuacagc uuauuucaug     3360

cuuguaaaau caagagacaa gaagaagaaa gauaaaacau uacuaagucu ggagugugua     3420

acuguucuag ccgacaaaaa uuuucguacu gcagaagaua aacuaaaaua uugugaaaaa     3480

gaacuugggu uaaaagagcc agaaauacuu auagagaaaa uaaaauuaaa cacuuuauuu     3540

aaaauaaaug gaagcuaugc guggauaaga ggacgaacca augacagguu aguacuaugc     3600

aacgcaaacc aauuauggcu ggauaaugga ucagcaaaag aacuaaagaa aauaucaaac     3660

cauuuccggg acgucaagaa auauaacaau aaggauuuac cgguaagaga agaagaauuu     3720

agcaaagaac aaaaucucgc acuauacgau uuguuuaccg aaaaauuagg uggaaaaauc     3780

uaugcaggac uuccaguaca aggccaaauc aaaaaccucg aaaaaggacg ggauaaguuc     3840

gaggcuuuag aacuaaaaga ucaacuauuu guuuuaaugg aaauucucaa auuuaugcag     3900

ugcaauucaa ugacuucgaa uauaaaguua aucggaggug ccgaaaaugc agggaaaaau     3960

agaauaucua aacuaauaca agacucggau guaaaaauga uauuuugcuc gccaacagga     4020

uacuucugua aaguagugga cuuuaaaaga uuucuauga                            4059


<210>  5355
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5355
augaagaaaa uacucggacu ugaccucggc acaacaucca uaggaugggc uuuuauaaaa       60

gaaccagaaa aagauguugu ggguucagaa auaguagaca ugggcgucag gauaguucca      120

cuuucaagug acgaagaaaa cgacuuugca aaaggcaaca ccauaucaau uaaugcagau      180

cgaacucuaa aacgaggugc aagaaggaau cuccaaagau ucaaacaaag aagaaaugca      240

cuccuagaaa uuuuuaaaga gaagaaacuu auaaguacaa acuuuaaaua cgcagaagac      300

ggacccucuu caacuuucag cacgcuaaau cuacgagcga aagcagcaaa agagaaaaua      360

gaacuacaag accucguaaa aguguuacua caaauaaaca agaaacgagg auauaaaucc      420

agccgcaaag caaaaagcga agaagaugau ggaagcgcaa uugauaguau gggaauagcu      480

aaagaacuau augaaaauga ccucacuccg ggccaauggg uauacgaagc auuacaaaaa      540

ggacgcaaaa acguaccaga cuucuaccgc ucggauuuac aagaagaauu uaagaaaauu      600

guaaacuauc aauccgaauu uuuccccgac auauucaacg caucauuugu cgaagauugg      660

augggaaaag cauccacccc uacuaaacaa uacuucaaca agaaaggagu acaacuagcu      720

gaaaacaaag gaaaaagaga agaaagaaga cuucaagaau acaaguggag ggcagaagca      780

guaaacuuca aaauagaccu cucugaaauc gcauuaauac uuagucaaau aaauagccaa      840

auuucaaaua gcucaggaua ccuuggagcc aucagugaca ggucgaaaga auuauacuuu      900

aaaaaucuca cuguaggcca auauuuauau caacaaauca agaaaaaucc ccacacccga      960

uuaaaaggac agguauuuua cagacaagac uaucuugaug aauuugaaag gaucuggagc     1020

guacagucau cauuuuaccc acaauuaaau gacgcauuaa aaagagaagu aagagauauc     1080

acaauauuuu uccaaagaag acucaaauca caaaaacauc uaauauccaa cugugaguuc     1140

gaagaccauc acaaaguagu acccaaaucg cacccagucu uucaagaguu ccgaauaugg     1200

caaaaucuaa acaauuuacu ucuuauaaag aaagauaacc ucaaugaaaa auucgaccuu     1260

gaacuagaau caaaaauagc acuagcgaac gaacuagcau uuaaaagaga acucaacguc     1320

aaagaugccu uaaaaauauu aggcuuaaaa ccaaacgaau gggaguucaa cuucacgaaa     1380

auagaaggaa aucgaacaaa ucaagccuuc uucgaugcgu uugcaaaaau aauagaacuc     1440

gaagacggug aaccaauuga ucuaggugac uugaaagcag augacauacu agaucaauuc     1500

ucagaggcau uuuuaagaau uggcaucgau accgaauuac uacaaguaaa uucagauauc     1560

gaaggagcag aauaugaaaa acagucuuac auucaauucu ggcacuuacu auauucuagu     1620

gaagaugauc agaaauuaaa acuuaacuug auccgcaaau ucggauuuaa accggaacac     1680

gcaaaaauau uagcaaguau cagccuacaa gacgaccacg ccucucucuc cucaagagca     1740

aucaagaaaa uacuacccca cuugcaauca ggccucauau augauaaagc cugcacauau     1800

gcuggauaua accauagcuc uaguuuuacu aaagacgaaa acgagaaaag agaacuacgc     1860

gcagaacuag aauuacucaa gaaaaacuca cuccguaacc cuguuguaga gaaaauacua     1920

aaccaaauga uuaauguagu aaaugcaaua cucaaagauc ccgaacuugg acgaccagau     1980

gaaauucgug uagagauggc acgcgaacua aaagccaaug cagaacaaag gaaaaacaug     2040

acgucuaaua uugccagugc gacaagagac cacgacaaau accgugaaau acucaaaagu     2100

gaauuuggac uaaaacgagu uaccaaaaac gaucuccuaa gauauaaacu uuggcuugaa     2160

acugacggca uaucacuaua uaccggaaaa cccauagaag ccucuaaauu auuuucaaaa     2220

gaauaugaca ucgaacauau aauuccgaaa gcgaggcuau ucgacgauag uuuuucaaac     2280

aaaacaauau gcgaaaggca acuaaacaua gacaaagcua acgucacagc guucucuuuc     2340

cuucaaaaca aauuaucagc agacgaauuu gaacaguacc aaagucgcgu aaaaucauua     2400

uauggaaaau uaucaaaagc aaaaauacaa aaauuacuaa uggcuaacga caaaauacca     2460

gaagauuuua uugcgagaca acuacaggaa acccgguaua uaagcaagaa agccaaagaa     2520

auacuuuucg aaauaagcag acgaguauca gucacaacag gaacaaucac agacaaacuu     2580

agagaagacu ggggcuuagu agaaauaaug aaggaacuaa auugggaaaa auaugauaaa     2640

cuaggauuaa ccuacacuau cgaagguaaa cauggcgagc gacuaaacaa aauaaaagau     2700

uggucaaaac gcaacgauca ucgacaucac gcaauggacg cucuuacagu ugcacucaca     2760

aaaccagcau acauacaaua ccuaaauaac cuuaacgcua aaggacuaaa caauaagaaa     2820

gguacagaag uauucgcaau agaacaaaaa uauuuaaaac gagaaaaugg aaaacuaugc     2880

uuuauucccc caauagaaaa uauaaggucc gaggccaaga aacauuugag ucgcauccua     2940

guuucauaua aagccaaaaa uaaaguagug accaucaaca aaaacaaaac aaaaucgaaa     3000

gcaggacuaa acgaacaaau ugcauuaacc ccaagaggac aacugcacaa agaaacggua     3060

uacgggaaau ccuuucacua cucuaccaaa uucgagaaaa uaggagcauc auuuaacgua     3120

cagaaaauaa acacagucgc gaaaaaggaa gaaagagaag cccuacucaa gagacuugca     3180

gaaaacggaa augacccgaa aaaggcauuu acaggcaaaa acacccuaaa caaaaugcca     3240

aucuacuuag aucucgggaa gaauauaaaa cuguccgaaa aaguaaagac aguaguacuu     3300

gaacaaaacu acacaauaag aaagaauaua gaccccgacc ucaagguaga caaagugauu     3360

gacguaggaa ucaaacgaau ccuagaauca agacucgaag aauuuggagg caaugcuaaa     3420

uuggccuuca guaaucuaga agaaaacccu aucugguuaa auaaagaaaa aggaauaagc     3480

auaaaacgag uuaaaauauc ugguguauca aauguagaaa gccuccacgu aaagaaagau     3540

cacuuuggcg aacccauucu ggaccaagaa ggaaacgaaa uuccaguaga uuucguaagu     3600

acagguaaca aucaucaugu ggcaauauau gaagaugaaa acggaaauuu acaagaagaa     3660

guaguaucau ucuuugaagc aguagugaga caaaaucaag gacucccaau uauuaagaaa     3720

aaccauacac ucggcuggaa auuccuauuu accuuaaaac aaaaugaaua cuucguauuu     3780

ccaucagacg auuuuguucc agccgaugua gacuuaaugg augaacaaaa cuaccaucua     3840

auaucaccca acuuauucag aguacagaaa auagcaagaa agaauuaugu cuucaauaau     3900

caucucgaaa caaaagcagu agauaaugac uugcucaaaa guaaaaagga acucucaaaa     3960

aucacauauc acuuuuauca aacacccgaa caucuccgag gcauaaucaa gauccgcaua     4020

aaucauuuag gaaaaauuau acaaaucggg gaauauuga                            4059


<210>  5356
<211>  4059
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5356
augaacaaga aauauuaucu aggauuagau auaggaacca acuccguagg uugggcagug       60

acagacgaaa acuacaaucu cuauaaauac gcaggaaaaa gaaugugggg aauuagacuu      120

uuugaagcag cagauacagc agcagaaaga agaaugaaaa gaucaaauag aagacgacuc      180

uccagaaaga aacaaagaau agaccuacuu caagaacuau ucucugaaga aauaguaaag      240

aaagaccuaa cauuuuucau aaggcuaaac gaaagcagac uccacuucga agacaaaagu      300

acuggagaaa aauaucccuu auuuguagau aaagaguaua cggauauaga cuaccacaaa      360

caauauccaa caauguacca ucuacgaaaa gaacuaaucg aaaauccaga gccacacgac      420

auaagacucg uuuacuuagc uugccaucau auacuuaaaa accgaggaca cuuccuaauu      480

aaugggucca uaagugacgu caaaaauuua ucauucguau gcggggaaau gauaaaagag      540

uucaauagcg ucagugaaua cgaaauaagc cugacagaua uaagagaacu agaaaggaua      600

cugagugaca agaaaacagc aaaaagcgua aaagccaaac aagucgcuca acuauucgaa      660

uguaccccug cagaggauaa agagcaacaa aaaugcgcca aagcaauaau cacaaaccuc      720

agcaaacuua uaguuggucu aaaagggaac auaaguaaaa uguuucauca accccuggaa      780

gagauaucua cuaaaucauu uaaauuuaca gaagccaaau acgaagauga aauaaugcca      840

gcgauagaag aaaacuaucc cgacuacgcc ucagucauug auagaauaaa agguguauau      900

gacuggagcg ucuuagugga aauacuugau ggagaagacu acuuauccuu cgcaaaaguc      960

aaagcauaug aaaaacacgg uaaaaaucuc cacauauuga aaaaugugau gaagaaauau     1020

ugcaaagaug aaguauacca agcauucuuu aauggaauaa augagaaaaa uggauaugga     1080

aacuacauag gugcaauccg uaaaaacgga cauacguaca aaauggacag gugcaaauca     1140

gaagaagacu ucuacaaagg ccuacagaaa auucuaagua caauaguacc cgaccaagaa     1200

gacgaagaac uccuagaaaa acuuaaagca gagguagaaa aucaaacccu ucuaccauua     1260

gcuagaucaa aagaaaacgg aacaauaccc aaucaaguac acuuacaaga gcucaugaaa     1320

auacuggaca acgcaaaaga auaccuagac uuucuaaaga aagaagauga auacggcaca     1380

auugcggaga aaauaauauc uauagcaaaa uucagaaucc cauacuacgu gggcccauua     1440

ucaagaagac auaaagaaga agguucuaac aguuggauga uacgaaaaga agaaggacga     1500

auauacccau ggaacuuugg agacaaagua gacguagaac aaaguaacaa agaguucauu     1560

gaaagaauga cuaauaaaug cacauacuug auuggagaag acguacuccc uaaaaacuca     1620

uuauuauacu cgaaauauau gguucuaaau gaauugaaua auuuaaaaau acgugggaac     1680

aaaauuucag ucgaacuuaa acagagaauc uauaaagauc uauucugcac cauggcaaaa     1740

gucacaggaa aaagaauacu agaguacuua aaaauggaag acccagaaau uguccaggaa     1800

gauauaagug gcuucgauau agacuucaaa ucugcccuag cuucauaccu agacuuuaag     1860

aaacaaguug ugggaaacga aaucgaaaaa gaccauuaua aagaaauaau ugaagaaauc     1920

aucaaaugga aaacaauaua ugaugacgau ucaaaaauga ugagacgaau gaucgaacgg     1980

gaauauccag aaguauuuga caaagaacaa auaaagaaaa ucugucaacu gaaauauucg     2040

ggauggggca acuucagcga cagguucuua aauggaguaa gaggagcaga uaaagagaca     2100

gaagagaguu auacaauaau agaggcccua uggaaaacaa auuacaauuu aaugcagcuu     2160

cuaucuaaac aauucagcuu caaagaagaa auagaucaaa uaaaugcaga gaaagcaggc     2220

gauguuggaa aaauuaacua cgaaaauaca gugaaagacc uuauaguauc accaucaaac     2280

aaacgagcca ucuggcaaac uauacaaaua gcggaagaaa uaaagaaagu gaugaaaugc     2340

gagccagaaa gaauuuuuau agagauggca agagguggag aaaaagagaa gaaaaggaca     2400

guaagccgga aaacuagauu acaggaacuc uacgcaggcu guaaagacga cacaagaaac     2460

uggaugaaag aaauaggaga cagagaagaa agagaguuca auaguagaaa guuauaucuu     2520

uacuauaccc aaaugggcaa augcauguac accggagacg aaauagaugu ugacgcuuua     2580

augaaaggca acagcaacug ggacaucgac cacauauauc cacaaagcag gaucaaagau     2640

gacagucuag acaacauggu ccuggugagg aaagauguaa acaauaagaa aaggaauaaa     2700

auuuuaucac caaaaaucag ggaagauaug aaaggcuauu ggaccaugcu gugucaaaaa     2760

ggucuaauaa gcaaaagaaa auaugauaga cuaacaagaa caacugauuu cacagacgaa     2820

gaacugucag gauucauuga aagacaauua guggaaacca gacaaagcag uaaagcagua     2880

gcagaacuac uuaaucgacu auacaaagau agugaagucg uauacguaaa agccggacuu     2940

guaagcgacu uuagacacga caaugaucua uuaaaaagua gaagaguuaa cgacuaucau     3000

caugccaaag acgcauuucu aaacauugua guagggaaug uauaucacgu aagauuuaca     3060

ucaaacccuc gcaaauggau caaagauaac agagacucaa auuauuccau uagacgugug     3120

uuuaauuacg acguuaaaca aggagacaaa auagucuggc augguaguga aaacggcgga     3180

cacaguauag accaaauuaa gaaaauaaug agccgaaacg acauccucua cacugaauac     3240

acguacugug aaaaaggcca acuuuucaac gaaacucuag ccaagaaagg aaaugaaaaa     3300

gcaaucgcac ucaagaaaaa cuuggaccca guaaaauacg gugguuauac aacucccaaa     3360

acuucugcau ucgcauucgu agaguucgau gguaagaaga aagaaaggaa aaaccauauu     3420

guagaaaucc ccguuuaugu agucaauaua gcuaaacgug aacccgacgc cguaaagaaa     3480

uauuuagaag aacgcaaagg auacucaaau guaguaauaa agaaauaucc uaucaaaaag     3540

aacucacuaa ucaaaaugga cgguuaccua gccagauuaa gaggagcaaa ugaaaaagac     3600

auuauacuca aaaaugcggu acaacuaaua cuuaauaaag acuuauauga aaucguccgc     3660

aaaauagaaa aauaucuaga auacaacuua caauaccaag cagaugcaaa auucgaagga     3720

uuuacaauga aagaccucaa caugauauau gaagaauuau accuuaaacu aaaagaguca     3780

auauuucaaa aacgaccugc ucaacaaaug aaaacacuag agaaaggcaa agaagccuuc     3840

auaucaauug aaaaccucgg ugaaaaggca aaaguaauaa acgaaaucau agcaauguuc     3900

agaugcgaug caacaacugu ggcgaaccua aaaguaauua auggcucaca aaacgcagga     3960

uccaugaaag uaaacaaaaa uacacucacu uccaaaucac uaaaauuagu acaucaauca     4020

auaacaggac uauucgaaac agaagaagaa cuuguauga                            4059


<210>  5357
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5357
augaaaaaga aauacuacuu gggccuagac aucgguacua acuccguagg augggccgua       60

acagacgaaa acuacaaccu augcaaauuc aacggcaaga aaaugugggg aauaagauua      120

uuugaagacg ccaaaacagc agcggaaaga cgaacgaaaa gaaguaaucg aagacgaauu      180

gcaaggacua aacaacgaau ugaucuucua caagaacuau gcgaaaaauc aauauuugaa      240

aaagacccaa cauucuuuau acguuuaaac gaaucauccc uacacuuaga agacaaauca      300

acgggagaaa aauauccauu auucguagau aaagaauaua cugacauaga cuaucacaga      360

gaauacccaa caguuuauca ccuaagaaaa gaacuuauug aaaauccaga accccaugac      420

cccaggcuag uguacuuagc uugcugucac auaauaaaau cacgcggaca cuuccuggua      480

aacggaucua uuagcgacgc gaaagaccuc ucauuuaucu gcgaaaacau gauaaacuca      540

uucaacgcug cuagcgauua uaaaauuaac auacaagaca ugacugcauu agaaacaaua      600

cugacggaca gaaaaauggc aaaaaguguc aaagccaaac aaauaguuca acuauucaaa      660

ugcauaccau gcgaagacaa agaacaacaa aaaugcugca aagcaauaau aacuaaccua      720

aguaaacuaa uaguaggacu uaaaggagac auauccaaaa ucuuuaacga gucauuugaa      780

gaaaucaaaa guuuuaaauu ugcggaaacu aaauacgaag acgaaauacu uccgacaaug      840

gaggaaaucu acccagauca cgcagaaaua auagagaaaa uuaaaucaau auacgauugg      900

agcauucucg acgaaauccu agauggagaa gaguacuuga gcuuugccaa aguuaaagca      960

uacgaaaaac acaagaaaaa uuuacaugau cuaaaaauuc uaauaaagaa auacugcgau     1020

gacgaaacau accaauccuu uuucaaugga augaaugaga aaaauaaaua cucaaauuau     1080

auuggcucga ucaggaaaaa cggacauaca uauaaaguag aaaaaugcaa aagcagcgaa     1140

gacuucuaca aagaacuacu aaaacuucua ucaucaguaa accccggaca agaagacgaa     1200

ucacuucuag aacaaauaaa auuugaaaca gagaaccaaa cucucuuacc acucuuaaga     1260

acaaaagaaa auggaacgau accgaaccaa guacauaugc aagaauugau aaaaauccua     1320

gauaacgcaa gcaaauauuu agacuuccua aaugaaaaag augaauacgg cacaacagca     1380

gacaaaauaa ucagcauagc caaauucaaa auaccauacu auguaggucc acuaagugac     1440

agacauaaag aaaacggauc aaauucgugg augguaagga aagaaaacgg aagaauuuac     1500

ccuuggaacu uugacgauaa aguagaugca gagaaaucga acaaagaguu cauacagaga     1560

augacgaaua aauguaccua ccuaauuggc gaagauguau uacccaaaaa uucauuacua     1620

uauucgagau auaugguauu aaacgaacua aauaaccuca aaauccgagg uaacaaaauc     1680

ucugucgaac uaaaacaaca aauauauaau gaucuuuucu gcacaaaagc aagagugacc     1740

ggcaaaaaua ucuuaaacua ccuaaagaaa gaggauccag aauuagcggc agaagaucua     1800

ucaggcuucg acguugacuu uaaaucagca cucacaagcu aucuagacuu uaagaaacaa     1860

guaguaggaa acgaaauaga aaaagauuac uauaaagaaa ucauugagga aauuaucaaa     1920

uggaaaacua uauauaacga agauuccaaa augaugaaac guaugauagg uagggaauau     1980

cccgaaacau uaaaucagga ucaaaucaag aaaauuugcc accuaaaaua uaacgggugg     2040

ggcaacuuca gugaaacauu ccuuaaugga auaaaaggag ccaauaaaga aacaggagaa     2100

ugcuauacaa uaauagaagc acuauggaag acgaauuaua accuaaugca acuucuaagc     2160

aaacaauucu cuuucaaaga agaaaucgac caaauuaaug cagagaaaac uggagcaauu     2220

gguaaaauua cauacgaaaa ccuaguaaaa gaucucgugg uaucacccgc aaacaaacgc     2280

gccauauggc aaacgauuca gaucacagaa gaaaucaaga aaguuaugaa augcgaacca     2340

gaacgcauuu uuauagaaau ggcaagagga ggagaaaaag agaagaaaag aacagucucu     2400

cguaaaacac gacuccaaga auuguauacg gguuguaaag augauacaag agauuggaug     2460

aaagaaaucg gcgaccgaga agaacgugaa uuuucuucua gaaaacuaua ucuauauuau     2520

acucagaugg gaaaauguau guauacgggg gaagaaauug acguagaccu uuuaaugaca     2580

gccaacucca aaugggauau agaccacaua uauccucaaa guagaauaaa agacgacuca     2640

uuugacaaua ugguccuagu ucgaaaagau aucaauaaca acguuaaagg aaacaauaua     2700

guaccaucuu caauacaaaa agaaaugacc agcuacugga aaaugcuucu acaaaaagaa     2760

uuaauaagua agaagaaaua ugaacgacua acgagaucca cagauuuuac agacgaagaa     2820

cuuagcggau ucauagaaag acaacugguc gaaacuagac aaucuucuaa agcaguggca     2880

gcacuguuaa acagauuaua caaaaauagu gaaauaguuu auguaaaggc aggcuugguu     2940

ucagacuuca ggcacgauaa caaguuacua aaaucacguc guauaaauga uuaccaccau     3000

gcaaaagacg cuuuccugaa cauuguagua ggaaauuguu acagaacaaa guucaccgcc     3060

aacccucgac aauggaucaa gaaaaacaga gacacaaacu acaguaucag acgaguauuu     3120

gacuuugaua ucaagcaagg cgauaaaaua gucugguaug guaauaaaaa cgaaaaugga     3180

aaacacacca ucgaccaaau aaagaaagcc augaguagga acgauauacu uuacacugaa     3240

uacacauacu gugaaaaagg aaaauuauuu gaugaaacac uaaccaagaa aggaaacaag     3300

aaagcaaucc cccucaagaa aaacuuagau ccuuuaaagu auggaggaua uacuucagua     3360

aagaccagug cauuugcauu uauagaauuu gacgauaaga agaaagaaag gaaaaaccac     3420

auaguugaga uacccauaua cguggcaaac aucgcaaagc gagaaccaga agcaguaauu     3480

aaauaucuag aagaacaaaa agguuacgua aacauuguag ucaagaaaua cccaaucaag     3540

aaaaauucac uauugaaaau ugacgguuac uuagccagac uaagaggcgg cgauagcuca     3600

auaauaacac ucaaaaacgc aguucaacuc guauuagaua agaaaaguua ugaaauaauu     3660

agaaaaguag aaaaauaucu agaccauaac ucaaaauacg aggcaaauuc uaaguuggaa     3720

aaauuuaacg aaaacgaccu gaauauuauu uauaaugaac uaugccuaaa acuaagaaau     3780

ucuauuuauc agaaaagacc uguacaucaa cuagaaacau uagaacaagg aauugacaac     3840

uuccgaucgc ucgagagcuu aaaggacaaa gcuaagauca ucaaugaauu auuggccaug     3900

uucagaugug augcaaaaac aggaauggau cuucgauuaa uaggaggagc aaguaauuca     3960

ggacgaaugc guauaucaaa aagcagacua acaucaaaau cauuaaaacu uauaaaccaa     4020

ucuguaacag ggcuuuucga aacagaagaa gaacuugugu ga                        4062


<210>  5358
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5358
auggagaaaa auuauuaccu aggauuggau auggggacuu caucaguagg gugggccguc       60

acagacgaaa auuacaaucu aaugagaaag aaaggcaaag acuuaugggg aguaagauug      120

uuuaaagaag caaaaaccag ugcagaaaga agaguuaaac gaacuucacg aagaagacua      180

caaagagaaa aagcucguau agguuaucua aaagaauuau uugcugauga aauaaauaaa      240

guagaugcag guuucuauca aagaauggaa gauagcaaau uuauaucaga ugacaaaacg      300

guccaccaaa aauucaccuu auucuccgac acaaacuaua ccgaugaaga uuauuacaac      360

gauuauccaa cgauauuuca ucuacgaaag aaacuaauag aaagugacga ugaauacgac      420

guaagaaugg ucuuccuggc acugcuaaac auguauaaac aucgaggaca uuucuuaaac      480

gcgaacuuau cagacaacgg gaugggagau auaaaugaac aauacgaucg uuuaaaagaa      540

cuuugcucag aauaucucaa uguagaaugg gaaaacaaac cagauaaauu cgaagaaaua      600

cuuaaagaau gcaacuguuc aaacucccua agaaaugaaa gauuacuaga aacauuuaau      660

cucaagaaaa cagauaaaga aaaaugugaa augcuaaaac uaauaugcgg acuaucagga      720

acacuaagca aaauauucaa agaccucgau gacgacucag aaaccaagaa aaugacaauc      780

agcuuuaggu ccuccaauua ugacgaagaa gcaauaaaug uggaagaaau cauaggcagu      840

gaaaacuucg aaauaauugc aguacuaaaa gaaauccaug auuggucgau gcucgcaaac      900

auaaugagag guaagaaaua uuugagcgua gcgaggguag aagaauacga aaaacacaaa      960

gaugaccuaa aaauacuaaa gaaagugauc aaagaauacu caauugaaga auauaauaac     1020

uucuuucgag uuauggcaga caauaacuac ucugcauacg uuaauagcgu aaauaguaac     1080

aaagaaagca caaggcgcgg agcgaaaugc gacaacgaca ccuucuuuaa agaaaucaag     1140

aaaauaguga auaaauguua ugauucagaa gaaaaagaau auauacuuaa agaaauagaa     1200

aaaggaacau ucuuaccaaa acaaauaaua aguucaaacg gaguaauccc aaaccaaguc     1260

cacaaaaucg aaaugaagaa aauacuacag aaugcugaaa aauacuuacc auuucucaca     1320

gaaaaagaug acucaggccu cucuguaucu gaacggauau uacaaauguu uucauuccaa     1380

auaccguauu auauugggcc aauaucugga aaaaucggaa gugacgagaa aagcaaaaac     1440

agcuggucug uacgaaaaga aaaaggaaga aucuacccau ggaauuucaa agauaaaaua     1500

gacgaaaaag aaagcgcuga aaaauuuauc gaaagaaugg uuaaucauug uacuuacuua     1560

aauaacgaaa ugguacuacc caaaaacuca cuucuguaug aaaaauucau gguucuuaac     1620

gaauuaaaca auuugaaaau aaacaaugaa aauauuucag uugaacuaaa acaaagccua     1680

uacaauaacu uauucaaaac aggcaagaaa guuacagcaa aagguauaaa aaguuaccua     1740

gaaagaaacg gcuuauguga gccaaacgaa gacauaaacu uaacaggaau agauggggac     1800

uucacaaaca gacucagcaa uuacgcaaaa uucaaaggaa uauuuaacac agaugaacua     1860

acauaugagc aauggaauau ggccgagaaa auaauaaaau ggucaacuau auacggagau     1920

ucaaagaaau ucuuggaaga gaaaauaaga aacgaauacu ccgaaaaauu ggacucucaa     1980

caaauaaaac gaauuuuggg aauaaaauuu agagauuggg gaagacuaag ugaagaacua     2040

cuaaacaugg agggagcgga uguuaacacg ggggaaauca aaucaguuau cggacgcaug     2100

ugggaugaga auuauaaccu aauggaauua aucgcuacgg acaauuucac cuacaaacaa     2160

guaauaaaag aaaaacaaaa cggacuagac aaaagucuau cagaaauaga uuaugacgac     2220

uuagaugagc uauauguuag ugcaccagua aaacgaauga ucuggcaaac gcuacuaauc     2280

auaaaagagc uagucagcgu gauagguuau gaaccaacaa gagucuucgu agagauggca     2340

aaagacguaa auacagagaa gaaacgaaca gauaguagga agaagaaauu uuuggauuua     2400

uauaaacagu gugaaaaaga ugaaagagau uuuuggauua aagaaaucga cgauaaaagc     2460

gaagcugaau uuaggaagaa gaaacuauac cuauacuaua cacaaaaagg aagaugcaug     2520

uacaccggag agcaaauaaa cccccuagac cuaaccaaug acaaccucua ugauaucgac     2580

cacauauacc cacgacacuu uguaaaagac gacucaaucg aaaauaaucu aguauuaguc     2640

aagaaagaga aaaacgcuca uaaaagcgau accuucccca uugaagauga cauaaggaaa     2700

aaccaauacg cuuucuggaa aaugcuauac gagaaaaacu ucauaacgaa gguaaaguac     2760

caacgauuaa ccagaaaaga accauucucc gaugaagaaa aagccaauuu cauaaugaga     2820

cagaucgucg agacccguca aggcacgaaa guaauaacag auauacuaga gaaaaguuuc     2880

gacaaaacac aaauaguaua uguuaaagca gggaacguau cguuauucag acagaaauac     2940

gaauguauaa aauguagaga aauaaaugau uuccaucacg caaacgacgc uuauuuaaac     3000

aucguagucg gaaacgucua ugauacaaaa uucacaagug aucccagaaa uuucauaaaa     3060

gaauacagga aaaaucccca aaagaacgaa uaccacaugu acaaauuauu cgacuacaac     3120

guucaaagag gcgaccgagu ugcuuggacu gcaucaaacg gggaaaguau gaaagucguc     3180

aaaggaacau uaaagaaaaa uacaccuuua guaacauuua uguccuacga agaacaugga     3240

ggacuuuaug accaaaacau cauaagugcg aaagauauag aaaaaguaaa aggaaaagga     3300

uauuugccaa uaaaaucgag cgacucaaga uugaauaaua cggaaaaaua cgggggauau     3360

gcaaaaguga gcggagcaua uuucuucgua guugaacaca cuguuaaaaa cguaagaaua     3420

aggacuauag aaccaaugcc cuguuauuug acaaaaguac uaacaacaaa agaaaagaua     3480

gaagacuauu guugcaauau ucuuaaauac gauagcccag uagucaaaua cgaaaagaua     3540

aaaauguaca gccuaauuaa ggucaacggg uacccaaugu accucacagg acgaacgggg     3600

aaccaguuaa uaguaucuaa uggaauacaa uuagugcuuu cauaugacga ggcgaaauac     3660

aucaaagacu uacuaaaaau aaaucaagag gaguuugauc aaaauagauu agaaaacuua     3720

ggaaucaaua aauuccgaaa cgagaaacua uacgacacuc uuaauaagaa auacacaaac     3780

gaaaucuaua agaaacgacc aaacaguaug ggcaacaaaa ugauagagug gaaaaauacg     3840

uuccaaaauu uaucaaucaa agaccagaca gaaguacuuc uccaacuuau ccaacuugga     3900

caaaaacaaa auagcggagu gaacuuagaa aaacuaggcg caagcaagaa aacaggaguc     3960

aguuuggugu caaagaaaau uacugauaaa gaaucauucg aacuuauaaa caaaucaaua     4020

acaggucuau augagaauaa aguugaucua cucagagucu ga                        4062


<210>  5359
<211>  4062
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5359
auggcugccu ucaaguauua ucucggucuc gacaugggaa caaacucagu uggcugggca       60

gucacagacc caaauuacaa ccuauuaaaa gcuaagggaa aagaccuaug gggaauucga      120

gaauuuaacg aagcauccac ugccguagaa agacggacuc acaggauuuc uagaagaagg      180

agacaaagag aacaaguuag aauaggucuu cuaaaagacu acuuccauga cgccaucgga      240

gaaguugacc cagacuuuuu ucaacgauua gcuaauucaa aauaccacuu agaggacaaa      300

gauacagaag uuagauacaa aaauaauguc uucaacgacg augacuacac ugauaaagac      360

uacuucgauc aauacccaac aauauaucac uuaagaaagg aauuaaucga aucaacagau      420

aagcaugaug uuagauuagu cuucuuagcg uugcuuaaua uguuuaaaca uagaggacac      480

uucuuaaacu cgggacuugg agaaaauagu ggugaaaaua auauaaacaa cgcuuaccuc      540

gaguuaacua accuacuuuc agaauucacu caauauaauc ugaacgaaac uauagacggc      600

aaaaaaauag aagacaucuu aagcagaaga gacaugucac gaacucgaaa agccgaaggc      660

uuagcggaaa ucuuaggugu cgacuacaaa aacaaaccau acaaagaauu aauaaggggc      720

cuuuguggau uaaaauucaa cgcaugcgca auauucccug aaauacaaag ugacgaagua      780

cccaaauuag acauaugccu gucggaagca ucguucgaug agaaaagcga ugaaaucgcc      840

aauauacuag gggaagacua uuucgaaauu auuauggcaa ugaaagacau uuacgacauu      900

ggaucauuag cagggauaau gaaaggucau aacuaccuuu cacaagcuag gguuacauca      960

uacgaaaaac auaaggaaga ccuaagauua cucaaaaaug ucauaagaaa auacugcagc     1020

aaagaagauu augauaauuu cuuuaauucc gaagcagaug gaagcuaugc auccuauguc     1080

gguucuuaca auucaaaaac aaaacaaaga cguguaggaa acaaaagguc cagugaggaa     1140

uuauacaaag caaucaaaaa aauucuaaaa aauguaccag cagaggacgc cgacguacaa     1200

aggauacuaa ugagcaucga aaacgaaaca uuuuugccga aacaauuaac ugccucaaac     1260

ggaguuauac caaaccaagu ucaugcgaaa gaaauaaaaa aaauauuauc aaaugcugaa     1320

agguaccuac cuuuccuacg agaaaaagac gaaacuggac uaucaguaag cgaaaaaauu     1380

augaaauuau ucucauucca aauaccauau uacguugggc cuacuacuga aaaaucgagc     1440

aaggacgggg gaaauggcug gguuguacgu aaagaagaag gacaaguacu accuuggaau     1500

auagacgaaa aaauagacau gaaagcgaca ucugaagcau uuauaucaag aaugguuagg     1560

agaugcacau acauaagugg ggagccagua uuaccaaaga gcucacuaga auaccaaucu     1620

uucuguguau uaaaugaaau aaauaacaua aaaauugacg gugaacgaau aucaguagag     1680

cuuaaacaag auauauacaa agaagucuuc caaaaaggca aaagggucac aaaaaaacaa     1740

uugugcaaau accuccacac uaggggaaua auugaagcgg aagagcaagu aacaggaaua     1800

gaugucacaa uaaacaacgc acuaacuacu uauggaaaau uuaaggcaau acugggagac     1860

gacauaucaa aagacagcgu gcaaaaaaug guugaaaaca uaguauuuug gugcacagua     1920

uauggagacu caaaaaaauu ucuaaaggau cgaauagaag aaaaauaugg cgaaaaauua     1980

acaaaagagc aaauaaaaag gauugugggg uucaaauuua aagauugggg aaaccuauca     2040

aaaaauuucu uagaauuaag uggagcagac guuucaacag gugaaucugu cuccauaaua     2100

cgagcuuuau ggaacaauaa ccucaaucuc auggaauuaa uaaauagcag acuguauaac     2160

uauaaagaaa gacucgcaga auaucaaaau acaaugauga aaacuuuaag ugauauagaa     2220

gcggaagauc uagacgaaua uuauuucagu gcaccaguua aacgcaugau auggcaaacu     2280

auucuaauaa uuaaagaacu gguuaaagua cuagggugcg aaccagauag gauauuuguu     2340

gaaaugacaa gaagaccaga ugaaagaaaa augagaaccg aaucuagaag aaagaaguuc     2400

gaagaacuau acaaaaaagu gaaagacgaa gacguagauu ggaugaaagu cauagcacau     2460

gcagacgaaa caggcucaau acgaagcaaa aaaauguacu uauacuuaac acaaaaagga     2520

agaugcaugu acacaggcaa acauauugag cuaagcgauc ucuuuaacaa uaaucuauac     2580

gauauagacc auguuuaucc gagacauuuc guaaaagaug auaauauuga uaacaaccuc     2640

gucuuaguau guaaggaaaa aaacgcacau aaaucagaua uuuauccccu agaagaugaa     2700

auacuagccu cccaaaaaaa uauguggagc gaauuaagaa gauccggauu cauuacugaa     2760

gagaaauaua accgacuaau aggacgcaac aguuucaccg acgaacaaaa agcagguuuu     2820

aucgcaagac aacuaguuga gacaagucaa gggacaaaag guguagcaaa cauucuacag     2880

caacuacuac cagaaucuaa aauuguuuau gcuaaagcuu caaaugucag ugaguuucga     2940

aauacuaggg acauuccaaa aagccgguua auaaacgaau uucaccacgc acacgacgca     3000

uaucuaaaua uuguaguagg aaauguuuac uauguaaagu ucacacagaa uccgcuaaau     3060

uuuauaaaaa acgauuacga ucgagacaaa acaaagaaua auuacaacuu aucaaaaaug     3120

uucgauuggg acguagaaag aaauggcgaa guagcuugga uagcacagaa aaaagauggc     3180

gaggcaggua caauugcaac aguaaaaaaa guacuaggaa gaaacacucc guuaaugacc     3240

agauacucgu uugaggguaa gggaggacuu acgaacgaga cccucuauuc agcaaaaaaa     3300

gcgaaagaag aaggauacau uccuuucaaa ucaagcgacg aaaaaaugaa agacguaaca     3360

aaauauggag gauuuacauc ggucacaggu gcuuauuuuu uucugguaga acauacagaa     3420

aaaaaaaaaa gaaguaggau gauagaaucc guaccgcuau auuuagcaca aaaaauagaa     3480

caaaauccgc acgaauugga aaaauacugu gaacaagucc uagggcuaau agauuuugau     3540

auacgaguaa gaaaaauuaa aauagguucc uuaauuaaaa gaaagggaua cuucuucuac     3600

aucacuggca aaacaggaca uagacuuaua uuacgaaaug caguaaacau gugcuuaaaa     3660

aaagaauggg uuaaauauau aagcaaacug gaaaaggcgg uagauaagaa caauguagaa     3720

gggauaauca ccaaagaaaa aaaugaacuc uuauauucag aauuaaucga aaaauucaaa     3780

aacagcauau ucaguaacag accaaaccca guuggugaua aguuagauga agcuaaagaa     3840

aaauucauua accuagacau agagaaacaa ugcaucgugc uaugccaaau auuacaauua     3900

agcaguguuc uaggaagcga augcgacaug accuuguuag gagaaagugc gcacugcggc     3960

aaaauauuaa uaucaaaaaa aauuggugau gaagacaucu acuuaauuaa ccaaagcgua     4020

acagggcuuu augaaaaaaa gauaaauuua cgaacuguau ga                        4062


<210>  5360
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5360
augaaaaaaa uacucggacu ugacuuaggu acaaacucca uaggaugggc cuucauuaac       60

gaagcagaaa acgaaauaga aguuucauca auuguaaaca ccggcguucg gaucguaccu      120

uuaacuaccg acgaagaauc agacuuuaaa aaaggaaaua caauaucaau aaaugcugau      180

agaacauuga agagaggcgc uaggcgaggc cuucaaagau ucaaacaaag aagagaagca      240

uuauuagaaa cauuuaacag aauuaaauuc auagcuaccg acuuuguaua cgcugagaca      300

ggagccgcaa caacauucag cagcuaccaa cuaagggcca aaucagcggu agaggcagua      360

accaaagagg aacucgugaa aguccuccuu augcuaaaca aaaaaagggg cuauaaaagu      420

ucaagaaaag caaaaacugc ugaagaagga gaugcuaucg acggaaugaa aauagcaaaa      480

gaaauauuug aaaacaaccu aacgcccggu caguggguau auaauuccuu gucuaaaggc      540

ggaaaauuca uaccggacuu uuaccauucc gacuuacaaa aggaacuaga gaaaauagua      600

agauuccaaa aucaauucca cucagagcaa auaaacgaca aauuacuagc agacauacaa      660

gguaaaucac ggacacaaac aucacaacau uucucuaaaa cacuuaauau accacuagca      720

gagaacaaag ggacucguga agaaacaaaa uuacagcacu aucaauggag aaacgaagcu      780

cuugcaaaug aacuagauau gaaaauacuu gcauucauaa uuacagaaau aaacaaccag      840

auaaaucaau caagcggaua ccuaggagcu auuuccgauc gcucuaaaga acucuacuuu      900

aaccaagaga ccguagguca auaccaauac aaacaguuac aggaaaacgu ccacgcaaaa      960

uuaaaaaauc aaguuuuuua uagacaagac uaucuagaug aguucgaaaa gauuugggag     1020

acucaagcaa aauuccauag cgaacuaaau gaaaaauuaa aagccgaaau acgagauauu     1080

acaauuuucu accaacgcaa acuaaaaagc caaaaacauc ucguaucauu uugcgaauuu     1140

gaaaaaggcc auaaagcuau cccaaaaagc ucaccauuau uccaagaauu uagaauaugg     1200

caaaaucuca acaacguagu cgugaaaaac gaaaccacaa aagaaguauu ugaacucagc     1260

gaagaauuga agcaacucau cgcagaggaa cuccaguuua aagaaucaau gacggauguu     1320

caaaugcuga cauuuugcga acuaaagaaa gggacacaca ccguaaacuu caaaaaaaua     1380

gaagggaauc gaacaaauac aagcaucuau aaagcauuug aaaaaauucu agaaguagaa     1440

ggauaugaaa uagacuuuuc aaaaaugaaa ccacuugaaa uaaaagaaac ugucgcuccc     1500

aucuucgaaa cccucaacau caacacagua auacuugacu ucgaucccac aauacaggga     1560

aacgacuucg acaaacaacc uuauuaccaa cucuggcacu ugcuguauag ugcagaagac     1620

gacgacaaau uaaaagaaac acuuaaaacc aaauucgguu uuaaagagaa ucaccuccca     1680

uuuauacuca auauaaauuu acaagcagau uacgguucau uaucggcaaa agccauaaag     1740

aaaauauuac cacauuuaau ggauggacac auauacgaca aagcuugccu uaugguagga     1800

uacaaucaca guucuaguuu aacaacagca caaaauaaug auagaguacu gaaagauacg     1860

cuagaacuac uuaaaaaaaa caguuuacga aauccagucg ucgaaaagau acuaaaucaa     1920

augauuaacg uaauaaaugc aauaaugaaa gacccagcca ugggaaaacc agacgagauc     1980

cgaguagaac ucgcaagaga acugaaggcu aacaacgaac aaagaaaaaa agcaaccucu     2040

gauauuaaua cagcaacaac agaacaugaa cgaauacgaa aacuacuaca uagcgaguuu     2100

aacaucccaa gaguaaccag aaaugacaua aucagguaua aacuauggcu ugaaacaggu     2160

ggaaucaguc uuuauacagg uaacccuauc aaagcuucug acuuauucuc uaaaaauuac     2220

gacauugaac auaucauacc aaaaucuaga uuauucgacg acaguuuuuc aaacaaaaca     2280

cuaugugaaa gacaacucaa cauagacaaa ucuaacaaaa cugcauacuc auuuuuacag     2340

gaaaaacuau ccgagaacga cuucgcagca uaugaaaaca gagucaaaga ccuauuuaaa     2400

acaggcaaaa uaaaaucaua cacaaaauac aaaaaauuau uaauggcaga caacgagauu     2460

cccgaaggcu ucauugaccg acaacuccgc gaaacacaau auauugcaaa aaaagcaaaa     2520

gaaauuuuac uugaaguauc aagaaauguc acagcuacua uuggaucagu aacagauaaa     2580

uuaagacaag auugggaacu aguagauguc augaaggaac ucaacuggga aaaauaugac     2640

aaacuaggau uaacacacau agaagaaggu aaaaaugggg aaagacuguc gaaaauaaaa     2700

gauuggacaa aaagaaauga ccaucgacac cacgcaaugg acgcuauaac cguagcuuuu     2760

acaaagccag cauacauaca auaucuaaac aauuuaaaug caaaaacaca aggagaaaac     2820

aaagccaauu ccauaauugg aauagaaaau aaacaucugu aucgggacaa gaacaacaaa     2880

cuacgauuca uuagcccaau gaaaaacuuu agagaagaag cgaaaaagca gcuugaaucu     2940

auacuuauau cauacaaagc caaaaacaaa gugguaacca agaauaaaaa uacaacuaaa     3000

aaaucgggag gaaccaacca aaaaauucaa cuaacaccca gaggacgacu acacaaagaa     3060

acaguguacg gcaaauugca acaauacgcu acaaaggaag aaaaaguaaa cgcuuccuuc     3120

acagaagacu acauacaaaa aguagccaaa aaagaauaua gagaggcacu acuuaagcga     3180

uuggaagaaa acgauaauga uccaaaaaaa gcguucacag guaaaaacgc ccuaaauaaa     3240

acaccaauau auauaagcuu gaaagacaac auaauaguuc ccgaaaaagu uaaaaccguu     3300

uggcuggaaa cagauuacac aauaagaaaa gauaucacgc cagaccucaa aaucgacaaa     3360

guaauagacg uuggaauaaa aagaauauua caaaacagac uuaaugaauu caacggggau     3420

cccaaaaagg cauuugugaa ccugggcgag agcccaauuu gguuaaacaa agaaaaaggu     3480

auugccauaa aaagagucac aauaucagga gucuccaaug cacaagcguu acacacaaaa     3540

aaagaccacu uaggcaauga aauccuugac aaaaacggaa acccaauccc aguagacuuc     3600

auaucaaccg gcaauaauca ccacguggcu auuuacaggg acgaaaaagg aaaucuucaa     3660

gaagaaguag uaaguuucua cgaugcugua auaagaagaa accuagggcu aaguguaauu     3720

aacaaaaacc acgagaaagg augggaauuu uuauucucca ugaaacagaa ugaauuuuuu     3780

auuuucccau cagacggauu caauccccac gaaauagauc ugcucaaccc caauaacuac     3840

caccuaauau ccccuaacau auuccgugug caaaaaauau caacgaaaaa cuauauguuu     3900

aaucaucauc uagaaacaaa agccgucgau ggcgaaaugc uuaaaagcaa aaaagaacuc     3960

ucaaaaacua gcuaccauuu uauccaaaca ccaaccaacc uuaacggaau cauuaaagua     4020

aggauuaauc acuuaggcaa gaucgugcac cuaggggagu auuga                     4065


<210>  5361
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5361
augaagaaaa ucuuaggacu ugaccuagga acaaauucaa uuggaugggc uuuuauaaau       60

gaagccgaaa acgaaauuga agugucauca aucgugaaua cagggguucg uaucguaccu      120

uugacaacag acgaagaauc agacuucaag aaagggaaca cuauaucuau aaaugcagac      180

cguacccuga aacgaggagc aaggcgaggc uuacaacguu ucaaacaacg cagagaagca      240

cuauuagaaa cuuuuaacag aaucaaauuu auagccacag acuuugucua cgcagaaaca      300

ggagcagcaa ccacauuuag uucauaucaa uuaagagcaa aaucugcagu cgaagcagua      360

acuaaagagg aauuagucaa aguacuacuc auguugaaca agaaaagagg guacaaaagu      420

uccagaaaag caaaaacugc cgaagaaggc gacgcuauag auggaaugaa aauagccaaa      480

gaaauuuucg aaaauaaccu aacaccgggu caauggguau auaauagcuu auccaaaggu      540

ggaaaauuua ucccagacuu cuaccacagc gaucuacaaa aagaacucga gaaaauaguu      600

agauuucaaa aucaauuuca cucugaacaa auaaaugaca aacuacuagc agacauacaa      660

ggaaaaagcc gcacgcaaac aagccaacac uucaguaaaa ccuuaaauau acccuuggca      720

gaaaacaaag gcacgcguga agaaacaaaa uuacagcauu aucaauggcg aaacgaagca      780

cuagccaaug aacuagauau gaaaauacuc gcauucauaa ucacagagau aaauaaccag      840

auuaaucaaa guucgggaua ucuuggggcc aucucagaca gaucaaaaga acuauacuuc      900

aaucaggaaa caguggguca auaccaauau aaacaauugc aagaaaaugu ucaugcaaaa      960

uugaaaaacc aaguauuuua uagacaagac uaccuagacg aguucgagaa aauaugggaa     1020

acccaagcaa aauuucacag ugaacuaaac gaaaaauuaa aagcagaaau aagagacauc     1080

acaauauucu accaaagaaa acucaaaagc caaaaacauc ucguauccuu cugugaguuc     1140

gaaaaaggac acaaagcaau ucccaaaagc aguccucuau uccaagaguu ccgaauaugg     1200

caaaaucuca acaauguagu agucaaaaac gaaacaacca aagaagucuu ugaauuauca     1260

gaagaauuaa aacaacucau ugcugaagaa cuucaauuca aagaauccau gacagacgug     1320

caaaugcuaa cauucugcga acucaagaaa ggaacacaca cagucaauuu caagaaaaua     1380

gaaggcaaca gaacaaauac cugcauauau aaagccuuug agaaaaucuu agaagucgaa     1440

gguuacgaaa uggacuuuag caaaaugaaa ccacuagaaa uaaaagaaac cguugcacca     1500

auauuugaaa cccugaauau aaauacaguu auacucgacu ucgacccauc aauacaggga     1560

aacgacuucg acaaacaacc uuauuaucaa cuauggcauu uauuauacuc ggcagaagac     1620

gaugauaaac ugaaagaaac ucuaaaaaca aaauuugguu uuaaagaaaa ucaucuucca     1680

uucuuacuaa acauuaacuu acaagcagau uacgggucac uuuccgcuaa agcaauuaaa     1740

aagauacuac cccaccuaau ggauggccau auauacgaua aagcaugccu aaugguuggc     1800

uacaaccauu caucaaguuu aaccacagcu caaaauaacg auaggguacu uaaagacacc     1860

uuagaacuac ucaagaaaaa cagccuaaga aacccaguag uagagaaaau ccugaaucaa     1920

augauaaacg uuaucaacgc aauuaugaaa gauccggcua uggggaaacc agaugaaaua     1980

aggguagaac uugcacgaga auuaaaagca aacaaugaac aacgaaagaa agcaacaagu     2040

gacaucaaca cagccacaac agaacaugaa agaaucagaa aauuacuuca cucggaauuu     2100

aauaucccga gagucacacg caaugauaua auucgcuaca aauuauggcu agaaacaggc     2160

ggaaucucau uauauacugg aaauccaauc aaaggaucag accuauuuuc uaaagacuac     2220

gauaucgaac auauaauacc aaaaucacga cuauucgacg acagcuucuc caacaaaaca     2280

cuaugcgaac gucaacuaaa uauagauaaa ucuaauaaaa ccgcuuacuc guuucuacag     2340

gaaaaguuau cagaaaauga cuucgcagcu uacgaaaacc gagugaaaga ccuuuucaag     2400

acaggaaaaa uaaaaagcua cacaaaauac aagaaacuuc uaauggccga caacgaaaua     2460

cccgaaggcu ucaucgaccg acaauugaga gaaacucaau acauugcuaa gaaagcaaaa     2520

gaaauacucc ucgaaguaag cagaaacgua accgcaacaa uaggaucagu aaccgacaaa     2580

uugcgagaag auugggaauu aguugacgua augaaagaau uaaauuggga aaaauaugau     2640

aaauuaggcc uaacacacau agaagaaggc aaaaacggag aacgauuauc aaaaaucaaa     2700

gacuggacaa aaagaaacga ccauagacac cacgcaaugg augccauaac cguagcauuc     2760

accaaaccag cuuacauaca auacuuaaac aaucuaaacg caaaaaccca aggggaaaac     2820

aaagccaauu caauaauagg uaucgaaaac aaacaucuau acagagacaa aaauaacaag     2880

cuaagauuua ucagccccau gaaaaauuuu agagaggaag cgaaaaagca acuugagagu     2940

auacuaauuu ccuauaaagc uaaaaacaaa guagucacga aaaacaaaaa cacgacaaag     3000

aaaagcggug gaaccaacca gaaaauucaa cuaacaccaa gaggacgauu acauaaagaa     3060

acuguuuacg ggaaacucca acaauacgca acaaaagagg aaaaagucaa cgccagcuuu     3120

acagaggauu acauacaaaa agucgcaaag aaagaauacc gagaagcacu acuuaaaagg     3180

cuagaggaaa augauaauga uccuaagaaa gcauuuacgg gcaaaaacgc gcuaaacaaa     3240

acaccaauau acauuagcuu aaaagacaac auuaucgucc cagaaaaagu uaaaacagua     3300

ugguuagaaa cugacuauac aauaagaaaa gacaucacac cugacuuaaa aaucgauaaa     3360

gucauagacg uaggaaucaa acggaucuua caaaaccgac uaaacgaauu uaauggugac     3420

ccaaagaaag ccuuuguuaa cuuaggcgag agcccaauuu ggcuaaauaa agaaaaagga     3480

aucgcaauaa aacgagucac aauauccgga guaagcaaug cccaagcccu acacacaaag     3540

aaagaccacu uagguaauga aaucuuagac aaaaacggca accccauacc uguagacuuc     3600

auaagcaccg gaaauaacca ccaugucgca aucuaccgag augaaaaagg gaacuuacaa     3660

gaggaaguug ucucguucua ugacgccgua auacgacgua acuuagaacu gaguguuaua     3720

aauaaaaacc acgaaaaagg uugggaguuc uuauuuucca ugaaacaaaa ugaguucuuu     3780

auuuucccgu ccgacggcuu caauccacau gaaaucgacc uucuaaaccc uaacaacuac     3840

cauuuaauau caccaaacau cuucagaguc cagaaaauau ccaccaaaaa cuacauguuc     3900

aaucaccacu uagaaacaaa agccguagau ggagaaaugu uaaaaaguaa gaaagaacuu     3960

aguaaaacau cuuaccauuu uauacaaacg ccaacaaauu uaaaugggau aauuaaagua     4020

agaauaaauc accuaggaaa aaucguucac uuaggugaau auuga                     4065


<210>  5362
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5362
auggcagauu uugcaaaaua ucaaccaggu auggucgcug acgggagaaa aauuccacua       60

gacuggacac uguauuaucu aagaaaaaaa gcgcuaacuc aagccauuag aaaagaagaa      120

cuagcaugga uacuucucaa cuucaaccaa aaaagaggau auuaucaguu acgaggugaa      180

gaagaagaag aaaauucaac caaacaugaa gaauauuaug aacuaaaagu aguuucggua      240

gaggcggcag aagaagguaa aggagggaau acaugguaca auguaaccuu agaaaaugga      300

uggaucuaca gaagacaauc aaaaauagaa uuaaacgauu ggguagguaa aguaagacca      360

uucauaguua ccacggaaua cgagauagau ggaguaacua uaaaaaaaga uaagaaugga      420

gaaguaaaaa gaaguuuuag agcaccuuca gaaaaugacu ggggcuugag aaaaaagcgu      480

accgaauccc uauuagaaga aucagguaaa acgguaggug cuuucauaua uaaccacauc      540

uuaucagaac cagccgacaa aauacgaggu aacuucauaa gaaccauaga aagaaaauuc      600

uacaaaaaag aacucgaagc aauacuucga gaacaaucga aacaucaaaa agaauuaaaa      660

gauauugaua cucuaaucgc uuguaucaag gaauuguauc ccaauaacaa accacaucaa      720

gacagcuuaa ucaaaaaaga caugacauac uuguuaauua cagaccuaau auuuuaucaa      780

agaccccuua agucgaaaaa aucguuaaua gcagacuguc cauacgaaua uuaugaguac      840

guaauaaaag aaaccgguga aaauaaaaga caaucaauaa aaugcauagc uaaaaguaac      900

ccauauuauc aagaauuuag acuauggcaa uucauaauaa aucuacguuu auucgaccga      960

aacaacgaua aagaagucac aagugaauac cuaagcucaa uggaagacua cacaagauua     1020

uuuacuuacc uaaacgauag aaaagacauu aaucaagaaa cacuccuuaa agauuuuuuc     1080

aaacaaaaaa aaguaaaacg ugguauggaa aaagaauuuc ccauucguug gaauuacaua     1140

gaagacaaag aaaaaaaaua ucccuguaau gaaacaagac augagcuuuu aacagcacua     1200

gggaaagucg gaauagacca cucgugguua gacgauaagg aacuuuuuua ccgguuaugg     1260

cacuuauuau auuccguaga aagcaaagaa gaagccgagg gagcacuaag aaaauuagua     1320

gacaacgaug auuucgugaa ugcuuuccua aagauaaaac cuuuuaaaaa agaauacggu     1380

gcauauagug aaaaagcuau aaaaaaacua uuagcaguaa ugcggauggg aaacuuaugg     1440

aacgaggugg acauauguga acagacaaga gacagaauac agaauauaau caaaggagau     1500

auagaugaaa aaauaaaaga ccgaaugggc agaacauuua gucaacuuuc agauuuucaa     1560

ggacuaccag aaugguuagc augcuauguc guuuauggac aacacaguga ggccacagac     1620

auacaacggu ggaccaaacc ggaaaaucua auggcguaca uuaacggauu caaacagcac     1680

ucgcuaagaa aucccaucgu cgaacaaugc auccucgaaa cacuacgcac aguccaugac     1740

aucuggaaag aagcaggcca cauagaugaa auucacauug aacuaggucg cggaaugaaa     1800

uccacagcug accaaagagc aaggaugagu aaaagcauaa ugcaaaacga aaauacaaau     1860

uuaagaauua aauccuuacu aauggaacuu aaaaaugcaa aagaauucga aaauguucgc     1920

ccauauuccc caaugcaaca agaaauauug agaauauacg aagaaggagc acuacaagaa     1980

cugacaaagg aagacgccca guauaacgaa auuagcaaaa uaucacaaau ggcacaacca     2040

ucagcaaaug aauugcuaaa auacagacuc uggcuugaac aaaaauacug uaguccauac     2100

acaggaaaac ccauaagccu auccaaacuu uuuacuagug cauaccaaau ugaacacaua     2160

aucccgcaaa gucgauauuu cgacgauuca uucacaaaua agguuauaug ugaagcagaa     2220

gucaaccaac uuaagucgaa cauguuagga uacgaauuua uaaagaaaca cgggggagag     2280

auaguccgau guacuaugau uggugacguc aagcucuuag augccaaaga auauuauaac     2340

uucguaacag agcacuacac caacaauagg agaaaaaaag agaaacuacu cauggaggau     2400

auuccucagg aauuuuuaaa cagacaaaug aaugacagua gauacauaag caaaaaggua     2460

auggcaauau ugagcaacau cguuagggaa gaagaugaac aagaagcaaa aucgaaauuc     2520

guaauagcgu guucgggagg gauaaccgau agguugaagc aagauugggg ucuaaacgac     2580

guauggaauu cuauagugua uccgcgauuu gagcgacuaa auaaacugac aaacacagag     2640

aacuuuggua gaugggaaaa caaaaaagga aaacaaguau uccaaacaag uguccccuug     2700

gagcugcaaa aagacuuuuc aaaaaaacgg auagaccaca gacaccaugc aauggaugca     2760

cuuguaauug ccugcgccag uaacaacaua auaaacuauc uuaacaauga aagcgcaaaa     2820

ucacaagaaa aaagggaaga ccuacggaaa aaauugugug acaaaaaucg aauaauaaga     2880

aaaccauggg aaacauucac gcaagaugca cauacagcuc uagaaaaugu gguaguaagc     2940

uucaaaaacu auauucgugu aauaaacaag gcuacuaauu acuaugaaaa auaugacaaa     3000

aaugguaaaa aagaaauagc agaacaaaaa ggacaagaua ugugggcuau aagaaaaccc     3060

augcacaaag aaacaguauu cggacaagua aaucuuagac gaaagaucac aauaaaauua     3120

aaagaugccu uagaaaaaau accugccaua ugcgauaaag aauuaagaga cuauauaaac     3180

auacuuguag caaaacaauu uaacaaaaaa cagaucuuag cacacuucaa auccaucaac     3240

uauagauuaa auaugaaaag cgucgauaaa guagauguau ggcaauuuag uaacgaaaag     3300

gagcccaugg ucgccacaag aaaaucugua gauacaagcu uugacaccaa aagaauagcg     3360

acgaucaccg auaccgguau ccaaaaaauu cuuuuaaacu acuuaaaagc aaaaaauaau     3420

gauccaaaca uagcuuuuac accagaagga aucgcagaaa ugaacaaaca uauaacagaa     3480

uacaauaacg gcaaacaaca ccaaccgaua cucaaaguca gaguaucaga accaaaagga     3540

gccaaauacc aaguuggaga aacagguaac aagcgaucaa aguuugucga agcucaaaaa     3600

ggaacaaauc uuuauuuugc cauauacgaa gacaaagaag gcaaucgauc cuauaacaca     3660

auaccacuua acgaagugau agaaagacuc aaacaagguu ugagcccagu acccgacgca     3720

aacgagaaaa guauaccauu aaaauuucac uuguccccua augaccuagu auauguacca     3780

aaaacagaug aggaaaauga gauaaaaaug ggacaaauau auaaauucgu agacagcagu     3840

gguacaacag caaacuuugu cccacacagg guaagcaauc ucaucuacaa cguggauaaa     3900

aaaauugcag aaaacuucug caaccacgaa auuauucaaa augaauacgg aaucggcuca     3960

ccacaaucca aaaaucaaaa aucaauuaca ggagaaauga uaaaagcagu augcuggaaa     4020

cuagaggucg acagacuugg aaauauacac aaaauaauaa aguga                     4065


<210>  5363
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5363
augaagaaaa uauuaggacu agacuuaggc acaacaagua uagguugggc auuuauaaaa       60

gaaccagaaa aagacguugu aggaucagaa auaguagaua uggggguacg gauaguacca      120

cuauccuccg acgaagaaaa ugauuuugcu aaagggaaca caauuucaau uaacgcagac      180

agaacauuaa aaagaggagc aagaagaaac uuacaaagau ucaaacaacg acgcaacgca      240

cugcucgaaa cuuuuaaaga gaaaaaucua auaccaucga acuuccaaua ugcugaagau      300

gguccaagcu caacauucuc aacauuaaaa cugagagaca aagcagcuaa agagaaaauu      360

gaacuagaug acuuuguaaa aguacuucuc caaauuaaua agaaaagagg auauaaaagu      420

ucaagaaaag cuaugucaga agaagacgaa gguacagcaa ucgacucaau gggaauagcc      480

aaagaauuau acgaaaauga uuuaacgcca ggacaauggg uguaugaagc acucaaaaac      540

ggacgcaaaa acguaccuga uuuuuaucga ucagaccuuc aagaagaauu uaagaaaaua      600

guaaauuauc aaagcgaauu uuacccugau auacucaaca caucuuucgu agaagauugg      660

augggaaaag caucgacccc aacaaaacaa uacuucaaca aaagaggugu acaauuagca      720

gaaaauaaag guaaaagaga agaaagacga cuccaagaau acaaauggag agcagaagca      780

guaaacuuua aaauugacuu aucagaaaua gcacucauac ucucccaaau aaacucgcaa      840

auauccaauu cuucagguua cuuaggagca auaucagacc guucuaaaga auuauacuuu      900

aaaaaucuaa cggucggaca auacuuguac caacaaguca agaaaaaccc ucauacacga      960

cucaaagguc aagucuuuua uagacaagac uaccuagacg aauuugagaa aauauggucu     1020

guacaaagca guuuuuaccc acaauuaaac gaugaacuaa aacaagaagu uagagacguc     1080

acuaucuucu uucaacguag acuuaaaucc caaaaacauc uuauaaguaa uugugaauuu     1140

gaagaacacc auaaagucgu accaaaaagu cacccaauau uucaagaauu ucgcauaugg     1200

caaaaucuaa acaaccucac ucuaaucaag aaagacaacc uaaaugaaaa augcgaauua     1260

gcacuagaag auaaaacagc acucgcgaac gaacuagcuu ucaagaaaga acuauccguu     1320

aaagaugccu uaaaaguacu agggcuaaaa ccguccgacu gggaauuuaa uuuuacaaaa     1380

auugaaggaa auagaaccaa ucacgcauuc uucgaagccu uugcuaaaau aauagaauug     1440

gaagauggug aaccaauaga ccuaggaaau cucaaagcgg augacauacu agaucaauuc     1500

ucugaagcgu uccuacguau aggcauagau acugaacuau uacaagucaa cagugacauc     1560

gaaggagcag aauaugaaaa acaaucauau auacaauuuu ggcaccuauu guauaguagc     1620

gaagacgauc aaaaauuaaa acaaaacuua auaaggaaau ucggcuuuaa accggaacac     1680

gccaaaauau uagcuucaau uucacuccag gacgaucaug ccucacuaag uaguagagcg     1740

aucaaaaaga uacuuccaca ccuccaaagc ggauuaauuu augauaaagc cugcacguau     1800

gcaggauaua aucacagcag caguuuuacu aaagacgaaa acgagaaaag agaacuaaga     1860

gcagaacuag aauuacuaaa gaaaaauagc cugagaaauc caguuguaga gaaaauuuua     1920

aaccaaauga uaaacguagu aaacgccaua cuaaaagauc caaaucuagg gagaccugac     1980

gaaauuagag uagaaauggc cagggaacua aaagccaaug cagaacaaag gaaaaauaug     2040

acgagcaaca uugcgagugc aaccagagau caugaaaaau acagagagau auuaaagaaa     2100

gaauuugguc uaaagcgcgu aaccaaaaac gaccuucuac gcuacaaacu uuggcuagaa     2160

acugacggga ucagucucua uacaggaaaa ccaauagaag caucgaaacu cuuuagcaaa     2220

gaauaugaca uagagcacau uaucccuaaa gcacgauuau uugacgauuc guucucaaac     2280

aaaacaauau gcgaaaggca guuaaauaua gauaaagcga acguaaccgc cuucagcuuc     2340

uugcaaaaua aacucucugc agacgaguuc gagcaguauc aaucaagagu caagagccuc     2400

uacggaaaac uguccaaagc caagauacaa aaacuccuga uggcaaauga uaaaauucca     2460

gaagacuuua uagcacgcca guuacaggaa accagauaua ucucaaagaa agcuaaagag     2520

auauuauucg aaauaacuau gagaguaagu guaacaacag gaacaaucac cgacaaauua     2580

cgagaagacu ggggccuagu agaaauaaug aaagaacuua auugggaaaa auacgacaaa     2640

cuaggacuaa ccuauacuau ugaaggcaaa cauggcgaaa gauugaauaa aauaaaagac     2700

uggaacaaaa gaaaugacca cagacaucac gcuauggaug cauuaacugu agcauuaacc     2760

aaaccugcau acauucaaua cuuaaacaac uuaaacgcca aagcaauugg aaauaagaaa     2820

gguacagaaa uauucgcgau ugagcaaaaa uaucuuaaac gagaaaacgg caaauuaugc     2880

uuuauuccuc ccauagaaaa uauaagggca gaagcgaaga aacauuuguc caaaauccuc     2940

guaucauaca aagcuaagaa uaaaguaguu acaauaaaua aaaacaaaac caagaaaaag     3000

gcagguuuca acgagcaaac ugcuuugaca ccaagagggc aauuacacaa agaaacggua     3060

uacggaaaga gcuuccacua cucaaccaaa uucgagaaaa uaggagcauc auuugacgca     3120

cagaaaauaa acacuguagc aaagaaagaa gaacgagagg cuuuacugaa acgccuagcg     3180

gaaaacggaa acgaucccaa gaaagcauuu acaggcaaaa acagcuuaaa uaagaugccc     3240

auauacuugg acuuaggaaa gaauauuaaa cuaagcgaaa agguaaaaac agugguacua     3300

gaacaaaacu auacgauuag gaaaaacaua gacccggauc uaaaaguaga uaagguaauc     3360

gacguaggga uaaaacgaau ccucgaguca agacuagagg aauuuggagg aaacgcaaaa     3420

uuagcauuca gcaaucuaga agaaaaucca auuuggcuaa acaaagaaaa aggaauaccc     3480

auuaagagag ugaaaauuuc agggguauca aauguagaaa guuuacaugu caagaaagau     3540

cauuucggag agaaaauacu agacgaagaa ggaaacgaaa uaccuguaga cuucguuagc     3600

acaggaaaua accaucacgu agcuauauau gaagacgaag augggaaccu acaagacgaa     3660

guaguaaacu uuuaugaagc uguaauccga agaaaucaag gugaaucaau uauaaaauac     3720

agacacgaag aagguuggaa uuuccuauac ucaauaaaac aaaaugaguu cuucgucuuu     3780

cccagucagg acuucaaccc aaaugacuuc gaccuucucg acccuaagaa cuaugacuua     3840

auaagcccua acuuguaccg agcccaaaag uucaguaagg ucauguacgg aaacucagcu     3900

guaagagauu acguauuucg ccaucaucuu gaaacaguac uaaacgacgu aaaagaauua     3960

aaggauguug cuuauaaaca auuaaaaagc cugcaauauc uuaaugguau uaagaaagua     4020

agacucaauc aucuugguca aauaauccaa guaggagaau acuga                     4065


<210>  5364
<211>  4065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5364
augacucucc auuauaacgu aggucuagau aucggaacaa auaguguagg cuauacaauu       60

guugaugaca auggaaaaau acuacaaguc aaaggcaaaa acggcuaugg aguacgcgua      120

uuuaaagaag gcgcaacugc agcugaacgc aggauuuucc gaacgacaca ccgcagauua      180

aaacgucgaa aauggagacu gcgcuuacua caagacuuuu ucgaaccaua cauauuacca      240

caagaugaug gauucuucau acgcagaaaa gagagcaauc uaguacuaaa cgaucgugau      300

gaagauacgg ccucguuauu caaugacaga ucagacagag auuucuauca agcauaucca      360

accaucuacc aucuaagaca agcacuaaug acagaaaaac gucaauuuga uguacgcgaa      420

auauaucuag ccaugcacca cauaaucaaa uauagaggcc acuucuuauu aaauggaacc      480

guuaacaauu ucacuaacaa caaaauagau cuccacagug auuucgaaga gcucaaugcg      540

cuacuccuca acguuauacc agaauuaaaa uuccaaauag accuagacca cucaacuaug      600

uuccaaacaa cccuacuaaa uacaacaauu agcaggucgg gacgacaacg agauuuaguu      660

aaaucauuuu auaacggaag ugaugauaaa caguuagcca aacagcaaaa aucaguagcc      720

accgaacucc uaaaagcaau acucggacua aaagcaaaau uucauaaacu uuuuaaucua      780

gacuuauuag agaccacaga augggaauuc ucauucgacg cugaagacau agaugacaaa      840

uuagcuaaac uggaaccuuu aaugacugag aaccaacaac aaguaaugga cauacuucaa      900

agaauauucg ccgcaauuac cuuaaacggg auugugccag aagguaagac acuaucagca      960

agcaaaguag acucauauca gaaacauaag caagaccuaa aauuacuaaa agcaaucgcu     1020

caacauucug auaaaaaaac ugcuaccguu cuacagacag ccuauaacga auacauuaac     1080

ggaauagcuu cgaaaccccu aaaacaagaa gauuuuuaca aacgacuaac aaacgcaguc     1140

aaaaaaucaa gcgacccuca aguagcagac augcucgcuc ucauagacca ggaaaaauuc     1200

cuaccaaaac aacgaacuaa agaaaacgga gccauuccuc accaacugca ccaacuagaa     1260

cuggaaagga uaauagacaa ccaaaaagau uacuaucccu gguuagccga acuuaauccc     1320

aaccaagcuc ggcaaaaagu agcgaaauac aaacuaagcg aacuaguagc auuuagagua     1380

cccuauuacg ucggaccuuu aauugaacca gaaguacaac aagccaccag uaacgcacac     1440

uucgcaugga uggcucguaa ggaagccgga ccuauaacac cuuggaauuu ugaccaaaag     1500

guagaucgua acguuagugc ugaaagauuc auaaaacgca ugacuacaaa agacacauac     1560

cucuuagcug aagauguccu accgcuacau ucccuauuau aucaaagauu cauaguacua     1620

aacgaauuaa acaacguccg agucaauggc caaaaauuaa caaaaaaaca aaaacaagcc     1680

guuuaccaag aucuauucaa acgacaaccu cacguaacaa aaaaacaauu uaaaagcuac     1740

uuaguucaaa ccggggaauu cgcagaaucc aguaaaauug aaggccuagc aaacgaaacc     1800

ucuuuuaauu cuggccuaac gaccgagaau gaacuccgua aaauauuugg agcacaauua     1860

gacgaagcaa gauaccaagu cgacuuugaa caaauaauag aaugggcaac acuuuucgaa     1920

gacgcuaaaa uccuaagaac caaacuagcc gaaaucacau gguugaccac cgaacaaauu     1980

gaaaaacuug cgggaauacg auaucgaggc ugggguagau uuuccagaaa acuucuagcc     2040

ggacuacgag aucaaaacgg acaacaaaua auagaccuau uaugggauac accaaacaac     2100

uucaugguca uagugucaca agcagccuuc ucugaagcca uaacaaaaga aaaugaaaaa     2160

cuaauugauc gccgcggagc gcaagacguu aucacagauu uguauacauc accacaaaac     2220

aaaaaagcuc uacggcaagu gcuagcuauu guagcagacg uucaaaaagc uaugggagga     2280

gugccuccuc aaagaauuuu uauagaauuc gcuagagaag acgaaaaaaa cccccgacga     2340

ucuguagaac gcucaagaca auuggaaaaa cucuaccaaa cuauauccaa cgaauuccuc     2400

auuaauucug aagugagaca agaacuuaaa gaagcaguag aucaaaaagu caauuucaaa     2460

gacagacuau uccuauauuu cuuacagggc ggaguggacu uauacucagg aaaaaggaua     2520

aauauugauc aacuaucaca uuaugauaua gaucacauac uaccacaauc auucauuaaa     2580

gacgauagcc uagacaaccg uguacucguu ucacaaaaac uaaaccguag caaaagcgau     2640

uccgugcccu uaucugaauu ugcgaacuau aaguucggac cggccaugca agccaaaugg     2700

cuucaacuua aagaagcagg ucuccucagc aaacggaaau augacaaccu cacuuuagau     2760

ccaacaaaac uaucaaaauu cgcaccucuc ggauuuguaa accggcaauu aguugaaacu     2820

agacaaguaa ucaaguuagc cgccaauuua cucgcauccg aagaaacaaa aguaguaaca     2880

auuaaagcca auaugacaca ccagguacga aaagaacuag auuuuccuaa aaaucgcaac     2940

gucaauaauu accaccacgc auucgacgcc uaccuuaccg cauucgucgg aauauuucua     3000

ucaaaaaggu accccaaauu aaaaccauau uuuacuuacg gcgacuucca aaaaggaaac     3060

aaacuuccug accuaaaaaa uuuuaauuuc cuauaugaac uuaaaaauaa agaccguagc     3120

auugacucua auacaggcga aaucaucugg gacaagcaac gcgaccucgc cuauaugaau     3180

aagauauaca acuuuaaaaa aguaacggua guccacgagg uguuaacgaa aucaggagca     3240

uuauacaacc aaacacuaua uaaagcuuca gaagacaaag ccaguggaag agguacaaaa     3300

caacuuauaa gaaaaaaaga caauaugcca accgaacugu acgggggaua uacagguucc     3360

accucugcuu ucauguccau cguaagacua uggaaaaaag acaaacccua cuacaaaguc     3420

guaggaauuc cuacacgaau ggcagccaag uuagcaaauc aagcacaacu auuagauuac     3480

uuaacaaaaa aauuuacaac acgaaaacua guaaaaaaaa caggugacua uaaaacaacc     3540

guagaacggu ucgaacuugu aguuccuaaa guugguuuua aucagcucgu uaucgacgga     3600

ggucaaccuu ucaugcuugg gucagcaaca uaccaauaca acgcaagaga acuauuuaua     3660

uccaaagaag caguuaaagc acuaaacaaa caacucucca cccacguuga ccuaguucaa     3720

guauuugaug aaauacucgu ccaaguuaac cgcugguuuc cccuauacga uacaaaugga     3780

uuucgcgaaa aacugucaca cggcagagac agauucauga aacugaauga cucauuugaa     3840

gaaaaaucag acacacaaau agauguacuc aacagaaucc ucauaggauu ccacgccaac     3900

gccgccagaa caaaccucaa aauauuaggu cuaggaacag accuaggauu caugacgcaa     3960

caggcuggca uucgccuaac cgaaaaagca auccucguac aucaaagucc uagcggacua     4020

uucgaacgca aaguagcacu aagagacuua aaaggccuaa gguga                     4065


<210>  5365
<211>  4068
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5365
augaccuacu ccaucggccu agacauagga acuggaagcg uugggugggc uguuauagga       60

gacgacuaca gacucaaaag agcuaaaggc aaaaacuuaa uuggaguacg auuauuugac      120

ucugcaacua ccgcugcuga gcgaagagga uacagaacca cucgcagaag auuaucaagg      180

cgacauuggc gccuaagacu acuuaacgac auauucgcga gcgaucucgc cgaacaagau      240

gaaaauuucc ucccaagguu aaaauacagc uggguaaacc cagaugacga gcaaaacccc      300

caacugaacu cagacgucgc aaauggagca cucuuuggau cgucagagac ugacaaggcu      360

uuucacaaaa auuauccuac uauuuaucau uuaagacacg aacuuaugac agacacugcg      420

aaacaugauc ugagagaagu cuacuuagca auucaccaca uaguuaaaua caggggaaac      480

uuuuuaaaua ccgcugacaa aauaaaugca gaaaacagcu uuaauguaga ccuuuucauc      540

gcugcauuag augaauacaa cgaauuucuu gaaaccccca uaacuagcau aaaagacgcu      600

cagcuauucu cagacaaacu gacagauaua aaugcaagga acaaaagcgu gagagucgaa      660

aaugcuaugu cagaaacaua ugacgacaaa agcaaaauau ucaaggcgau acuuacagcc      720

cuagugggaa auacagcuaa cuuucaaacg auauucgacc uuucagaccu agaaaaagac      780

gaagcuaaaa auuucaaauu caaacucgac uuagaagacg uagaaacgaa acucgacgua      840

guaagggcaa aauuauccga ugaacaaaac gcauucuuag aagcaguauu gauagcauac      900

gauggaauaa cacuaaaaau gauacuaggc gacuacaaau caauauccca guccaugauu      960

gaaucguaca aaagccacca acgugauugg aaauauauua aaaauaacgu gagacuaaac     1020

gaacaaaaga uagaacccga caaaaacagu guaaaauucu ucaaauuaga ccccaagaaa     1080

cuaauauauc cauccaaucu agcauaucuc caaguaauau ccucaaauga agaugaacau     1140

agaaaaggau uagacuacuu uaaaacagca auacaagacg cagauauccu acaacgaaua     1200

gaaaacgaca cauuccuccc uagacaacga acgaagcgaa acggaguuuu accauaccag     1260

uuacaccucg cagaacuuga acagaucauc aaaaagcaau caaaauacua uccuuuucuc     1320

gcaaacauau acacacaaga caauaaaaca gaaaauaaac uaauaggucu auugaaauuc     1380

agagugccgu auuaugucgg accacucgug gaggcagaaa aaguaacagg agauggcaaa     1440

aaucauuggc uuaucaaaaa ggaacuacaa gaaaauaccc ccauaacacc auucaauuuu     1500

gaugauguug uagauaaaga uaaaagugga gaacaauuua uaaaaagaau aacaggcacg     1560

gauaguuacc uaauaggcga accgacccua ccagagaaca gucuccucua ccaaaaauac     1620

aacguacuac aagaacuaaa caaugucaga aacgaaagac accaacgacu gccaauaaaa     1680

guaaaacaag acauauuuga acaugucuuc aaaauagaca aaaacguaaa cgcaaagaaa     1740

guaauggcau acuuaaaauu aaauggcuau ggcgacuucc aaauugccgg ucuuucagac     1800

acucgaaccg ggaaauuuaa uuccaaccua ucguccuaua acuacuuugu ugauuuauua     1860

ggaagagaaa agacagagaa aauaagagaa gauagguuag aagaaauaau cgaaauacaa     1920

acaguuuucg aagacaaaau acuugucgcc aaacgacuaa gcaaccuaga cuuccucaca     1980

ccccaagaga aaagcaaacu uagcgcuaaa cacuacaccg guugggguag acuaagccaa     2040

aaauuauuaa ccacacaauu cauaaaaaca gcuuuaagcg aagcaggaga caucauucca     2100

caaucacacu ccauaauaga cuuauuauau uacacagaua aaaauuuaau ggaaauacua     2160

aacgauaaaa cauucaaugu cagaaauugg uuaaccgaac aaaacaaagg cacggaauca     2220

ggaaauaguc uauacgauca gauagacgca uuagcuggac ccaaagacau uaaaagagga     2280

auaacgcaag uauuuuccau uauugaugac auaaagaaag caauggguag uaauccagaa     2340

acuguauacu uggaauuugc uagagaaaca caaacaucac gcuuaacaaa uagccgauua     2400

aaccguguac aagaaauaua caauucacca gaacuaaaaa cagaguucgc auaucuagca     2460

aagaaauuag aagcugaaac caaagaaagc auucaugaug accgccuaua uuuauauuau     2520

uuacaagaag gacgugacau guauaccgga aaagaaauac cuuucgauca aaucucauca     2580

aacuaugaca uagaucacau aauaccccag gcauucacaa aagacgauuc auuagacaau     2640

cgaguguuag uaaacagagc aaccaaugcc cgaaaaagca acucaccaac auucacaaau     2700

gauauaauag acaacaggaa aucuuggugg caaucacucg uaaaaagggg uuuaaugucg     2760

aaagagaaau uugaaagauu aacggauaaa agaaaaaccu ucuccgaaaa ccaaacaaac     2820

cacuucauug caagacagcu aguggaaacu cgccaaauca ucaaaaacgu agcugaucua     2880

aucaaaaaua auuacgaugg aacacaagcc guugcaauac gagcuucauu aacgggcgaa     2940

augagacgau aucuagacug gccuaaaaau cgggacauga augacuacca ccaugcgcau     3000

gacgcacuua ugauggcaac aguugggaaa uacguagaua gacgcggauu cuucgacaau     3060

ggaucaguuu cagacggagc aggcaaugca uauaaccucu acacuaaaga uugguuagua     3120

gcagcgagag uaggcacaag aaacgauucu caacgaguaa auccauacgg auuuauagua     3180

ggaucaauga acucaaaagc aguaagacaa caagucaaca aagaaacugg agaaauagua     3240

ugggggaaug aaaaccuaga uuacuuacga cacguauuaa gcuacaagaa aauacucguc     3300

acucgacguu uaucaggauc uucgggacgu uuauacgcag aaacacuauu uaguccaaac     3360

ccaaaagcga aacugauagc acaaaaagca aacaggucag uagcucuuua cggaggauuu     3420

acuaaggcug aaagcgcaua cacggcccua guagaauuua acaaaaaggg agaccgauca     3480

uacaaacuga ucaaaguacc aauggcaauc gcaaacagag ugaaugacaa acaaaucaca     3540

guaacagcau auauugcauc acuuaacuua agggacuuca guaggguagu acuagaacac     3600

uugccauuag guacacucau ccgagaaucu gacggaagcg aauuuuacgc agcaucaucc     3660

gaauauaaac acaaugcaaa agagcuuugg gcucccaaag aaauucuacg uuccagcaag     3720

aaauuagaca aagccaccgc ggacaauuua aucgacauuu uugauuuucu gacuaacgau     3780

aaaacaaucu caaauuuuaa auucuacgaa aaagaucuuc uucaucucaa aaacguaaga     3840

gauaaauucg uagaggcuuc agacgaaauc aaaauuaaag uacuaucaga uguuauuuau     3900

gaauugcaca augaugccgg auggagagac ccauucaaac acauacaaga aaaaccggcc     3960

uggacuaugc uccaagucaa agguggaaua aaacuuucag aaaaagccgc aauagucuac     4020

caaucaccca cgggguuauu cgaaacuaua acaauagugg gggaauga                  4068


<210>  5366
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5366
augaaaaaau uugaagaaua cuacauuggu cuugacaugg gaacaggaag cguagguugg       60

gcuguaacag acucagcuua caacaucauc aaaagacacg gaaaagcacu auggggaauu      120

cgucuauuug aaucugccaa gacagcugaa gaaagaagaa uauuucgaac aagccguaga      180

aggacagaaa gacgaaaaaa ccgcuuacau uuauugcaag aaauuuuugc agaagaaauu      240

aguaagaaag acaugggcuu uuaucaaaga augaaggaaa guaaauauug gcacgaggau      300

aaaaaagaca uaaauggaaa caugccugaa cuuccauacg cccucuuugc agaugauggu      360

uuuacagaca aagauuauua uaaaaaauau ccaacaauuu uucaucucag auaugcucuc      420

gcaacaaaca caacagauaa accugacauc agacucuuau accuugccuu acaucauaua      480

auaaagcaca ggggucacuu cuuauuugaa ggaaaaaaau uaagcgaggu gaaagaauuu      540

ucuacagcaa uagaagcucu aguacaacaa gcggaagaac aagaaauuga ccuaaagagc      600

cuaacccaag aagaagugcu aacacaauua gaacauauaa ugagugaugc ucaauguaca      660

aaaucagaua aaaaaacaca gguaaguaaa cuuauuguag gaacagauaa acagaaacaa      720

aaacaagaga aagcaauagc ugcauuaauu acaggcugua caguaaaauu aucagauaua      780

uuugccgacc cagaacuuga uaaagaagaa aucucaaaaa uauguuuuuc agauaacgga      840

uacgaagaaa aggaaguuga aauagaauca guacuagcag acagauuccu ucuuauagcu      900

gccgccaagg gagucuauga cuggacggua uuacaugaca uucuagggga cgcucgauuu      960

uugucagaag caaaaguaaa aaccuaugaa aaacacagag augaucuaag aaaagugaaa     1020

caacuacuaa gaauggauaa agaaguguac gaggccguuu ucgguguauc agaaaaaaau     1080

gaagcuaauu acagcgcaua uauuggaaug gcuaagaaaa auggcaaaaa acaaccuaua     1140

gaaaaaaagu gcagccaaga agauuuuuac aaauuccuua aaaagcaauu agaccgucua     1200

aguguugaug aaaugggaca ggaaauaauu gaugaacuua aagcggaaau ggauuugggu     1260

acgcuauuuc cgaaacaaau gauacgcgau aacggaguaa uaccuuacca auuacaugaa     1320

aacgaacuca acgccauacu agacaaugca cagaaauucu auccauucuu guuagaaaaa     1380

gaucaggaug gguacacacc aguagaaaaa auacaaaaaa ucuuuagguu uagaauaccu     1440

uauuacguag gaccacugaa uacagcacac caagauaaag gaggacacag cuggaguguu     1500

cguaagaaag aaggcaaaau auacccuugg aauuuuagcg agaugaucga ugaagaagaa     1560

ucagcaacaa aauucauuga aagaaugacu aauaagugua cauauuuaau aggagaagac     1620

gucuuaccaa aggaaucuuu auuguaugca aaauucaaug uacuaaacga acuaaauaau     1680

cuaagaauag acggcgaauu gaucgcuccu gaauuaaaac aggacauuua uaacaaugua     1740

uuuaaaaaau accaaagggu aacgggcaaa aaaguaaaag acuacuuaag aauaaauggu     1800

gucauaucaa aagaacaaga gcuaagcgga uuugaucaaa acuuuaaauc uaguuuaaaa     1860

gcuuaucacg acuucaagca gauaguagga acaggcgucc ucagcgaauc ugaacaagaa     1920

gauauuguac gcgacauaac ccucuucgca gauagucaaa aaaugcuaaa acaacgaauu     1980

cacagaaagu accccauguu guccgacaaa caaauugcac aauuagcgaa caaaaaauac     2040

gcaggauggg ggaggcucuc ucgaacauuc uuagaaaaua uagaaagcgu gaacaaggaa     2100

acgggagaag ucaugaauau uauuacagcg uuaugggaga cuaacgaaaa cuuaaugaaa     2160

uuacuuagug gaaaauauga uuauacuaaa caaaucgaac aauggaaugc gaggcaaaug     2220

aaaaaagggg aaauagaaua ccaagauaua gaagaguuau auguaucacc cgcagucaaa     2280

agaccuauau ggcaaacaau aaagauugug cgagaaauua caaauauaau gggaggagca     2340

ccaaaacgaa ucuucguuga aguggcuaga gaaccaggag aaaaaggagu ccguaaagua     2400

aguagaagaa accaacuucu agaucuauac aaagcaugca aaaaagaagc acccgaauug     2460

uacgauagac uugccaauaa ggaaucugau aaugcauuaa gaucagauaa auuguuccua     2520

uauuauucuc aaaugggaag guguauguac ucaggugaug cuauagauuu ggacgaacug     2580

uuuaccaauc gauaugauau ugaccauaua uacccacaau cuaaagugau ggaugauagu     2640

auugauaaua ggguacuagu aaaacgcgac uuaaauagua aaaaaacaga caccuucccc     2700

gucucagagg aauacaggaa agcugguaaa gcauuaugga gcacucuacu aaaaaaaggu     2760

cuaauuucca aagagaaaua uaaacgacua acaagaaccg aaccuuucgg ugaagacgaa     2820

cuagcagguu ucauugcaag acaacucgua gaaacacguc aauccacaaa agccgucgca     2880

cacuuacuag aagaagcaua uccugaugca gaaauaguuu augcuaaagc ccgcgcaguu     2940

ucagaauuca gacauaaauu ugauaugaua aaaguaagag agguaaacga uuuccaucau     3000

gcuaaggaug ccuaccuuaa cauaguugua ggaaacacau acuacguuaa guuuacgaaa     3060

gaugcagccu gguucguaag aaacaaucca ggacgcacuu acaauuuaaa aaagauguuc     3120

gagaacgaaa ccgucaagcg gggaaaugaa acugcaugga uacccggaga uaauggaacg     3180

aucuguacag uaaaaaaaug gaugcgaaaa aauaauguau uauuuacucg gagagcuuau     3240

gaagucaaag gaggucuauu ugaccaaaac auccugaaaa agggaaaagg ucaaauaccc     3300

auuaaaggag gugcugauga aagauuaaga agcagagaca aauacggagc uuacaauaaa     3360

gccucaggcg cauauuucau guuagucgaa agcgacggca aaaaagguga acgaaaacga     3420

acaauagaau ucguccccgu auaccuagcu aaaucccucg aagaauccgu agaacuaaga     3480

agaagguacu gugaagaaga acuaggacua guaaauccgg augugcgucu aagcaaaaua     3540

aggaaaaaug cacucauaga cguagacggu uucaaaaugc accuagucgg uaggacugaa     3600

aauagguuau uacuaauagg ugcgacacaa cuagugcuca gcgaagauga ccaacaauua     3660

aucaaaaaaa uuggaaaaua cugccuagac caucgagaga acaaagaugc aguauuaagu     3720

gaaaaauacg aacugacuau ggaccucuuc gaaaaacuuu augauauauu uguauuuaaa     3780

cuagaacaaa caguguaugc agucaaauug agcaaacaag ccaaagacuu aaaagaagua     3840

aaagaagaau ucuuagcacu ggaugaugaa caaaaaugcu uauuauugau ggaaauacug     3900

cauuuauuuc aauguucacc acaacuagcg aaucugacaa auauaggugg agggaaaagc     3960

gcaggaucac uaaaaauccu cuccgauaua acaaaacaac aaucaguacu uuuaauaaac     4020

caaaguauaa caggauuuua ugaacaaguc auagacuuaa aaacuauaug a              4071


<210>  5367
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5367
auggaagucg gaaacuauua uuuaggaauu gaugcaggaa cagacagugu uggcugggcc       60

guuacagaua aucaauacaa ugugauaaga aagaaaggga aaucacuaug ggguauaaga      120

cuauuugaug gcgcuaauac ugcccaagaa agaagaacau uccgcgcacu acgacgacgu      180

aacgaacgaa agaaacaaag aauuaauuua uugcaagaac uauucuguaa agaaauaugc      240

aaacuagacc ccaauuucuu uauaaggcuc agagacucau cccuaugguc cgaagauaaa      300

gaagaaaaac aaauauauuc ccuauuuaac gauaaaaacu uuaaugaccg caauuaucau      360

aauaacuaca aaacuaucua ccaccuaaga gcagaccuaa uuaaaaacaa agaaaaacac      420

gaugcacgcu uaguguacuu agcaauucau cauauaauga aaaaccgcgg ucacuuuuug      480

uuuaauggaa acaucgaauc cgucaccuca uuugaaaaua cauucaacga auuuaaaaac      540

ugucuacuug augaacuuga aauaaacuua gaguguccau caauaucuga guucgaaaac      600

auccuaaaag auagaacauu caaaggcacu caaaaaugua agaaacuaaa agaacuuugc      660

ucgauuccaa guucagauaa gaaauuagau aacaucuuca aauuaauuuc aggacuaaaa      720

gcugauuuau caauaaucuu ugaagacgag ucuuucaaag aaaacgaaca uaacaaaaua      780

ucauucgcag acaguaacua ugaagaaacc agggaaguau uaaauaguga aauacaagag      840

aaaacacuga uaauagacau auuuaauagu auauacaacu ggguaauauu aucugauaua      900

cuauccggug gugaauauaa cgguggacua uacuuaagca uagcuaaagu aaauaccuac      960

aauaaacaca guaaagaccu caaaauauug aaaagcguua ucaagaaaua cuucccucaa     1020

aaauacacag acaucuucgc cucaaauaaa gaaaauaacu acuguagcua cauaggcaug     1080

uacaaaucgg gagguaagaa auauucaguc aagaaaugcu cagacaaacc agaagaguuc     1140

uauaaaacua ucaaaaacau aauaaauaaa aauacagcag aagaaauauc aaaugacaaa     1200

gauguacaau acauucuguc agaaauagaa aacaacucau uucuaccuuu acaaauaaau     1260

aaaaacaacg gagucauacc auaucaagca aauaaaaucg aacuagaaag cauacucaaa     1320

aacgcagaaa acuaucuacc uuuccugaaa gaaauugaua ccgaauguaa caaaaccaca     1380

ucagagaaaa uaauagauuu auucgaguuc aggauuccau auuacguagg accccuuaac     1440

acuucaaaag gugaaaacgc auggaugaua agaaaagaag auggaacaau ccgaccgugg     1500

aacuucgaac aaaaaguaga cauagacaaa acggccgcug aauuuauuac aagaaugacg     1560

aaccaaugca cauacuuaaa uaaugaaaca guauuaccaa aauauucacu cuuauauucu     1620

gaauacacgg ucuuaaauga aaucaauaac auccguauua acggagagaa aauuucagua     1680

gaacuuaaac aagacauauu caacauauug uuuaaaaaua acaaacaagu aaccugcaag     1740

aaauugcugu cacaucuaaa uucaaacggg uacaacuuaa agaaagauga ucucucgggg     1800

uucgacggga acuuuaaauc aucgcuaagu agcuacauag aucugaaaaa cuauaucuuc     1860

ggagacaaca uagacaaacc ucaaauauac aaucucgccg agaaaaucau ccucuggaua     1920

acauuauaug gagaugacuc aaaacuacua aaacggguuu uacagaaaaa uuguagcgaa     1980

caacuuaacc ccgaacaaau caagaaaaua accaaauuaa aauucaaagg auggggaaga     2040

uuaucaaaag cauuccuaag ugaauuacga ggggcagaua gagaaaccgg agaaacaaug     2100

acuauaauaa aaggccucag aaacacucaa aacaaccuaa ugcaacuucu aucacaaaac     2160

uacaccuuca cagaagaaug cgagaaaaua aauucagggu acuucauuaa uccaaacgaa     2220

aucucauacg auuccauagu uaaaaacaua guagcaagcc caucaauaaa acgaaguguc     2280

uggcaaacaa uacaaaucgu cgaagaaaua caaaaaguaa ugggaucaaa accacgaaaa     2340

auauucguag aaauggcacg aggagaagaa gaaaaagucc gaacaacguc aaggaaacaa     2400

aaacuacuug accuauacaa aaauauaucu gacgaaugcg ccaaaggaau guacgacgaa     2460

auauauucaa aagacgaauc cgacuucaaa aacauuagau uauaccuaua cuauacacaa     2520

augggauguu guauguauag cggaaaacau auugacuuaa gucaauuauc caacacaaau     2580

auuugggauc gagaccauau auacccacag ucaaaaacua aagacgacag ccuagauaac     2640

cuaguacucg uauacaaauc gauaaauucu gagaaaucag acgauauaau auccccuaca     2700

auccaaaaua acaugagaac cuuuuggaaa cucuuauucg acagaaaauu uauaagcgaa     2760

aagaaauacc acagacuaac aagaacaaca ccacuaaccg acgaagaauu agccggauuc     2820

auaaaucgac agcuagucga aacaagacaa ucaacuaaag uaauggccag uauauuuaaa     2880

agaauuuacc cuaacacaga aaucaucuau guuaaagcca aaaacgucuc agacuuuaga     2940

caucgaaaag acugcgaaaa cggcguuaaa guuagagagu uaaacgauua ccaucacgca     3000

aaagacgcau acuuaaacau aguaguagga aauguuuaua gcaguaaauu uaccaauaac     3060

ccgcuaaugu ggcucaaaaa uaaucgaaac gucaaauaua gccuaaacag aauguacgac     3120

uucaaccuca caguaaacaa ugaaaacaua uggguaguag guaacgaggg cacccagaaa     3180

acgguaaaua aauaucuagg caaaaacgau auacuauaua cacguuaugc aaugaccaac     3240

aagggugaau uauucgauca aaacccacuc aaagcaaccg gagacaaauc caaacaaacc     3300

accuuaguac cucucaagaa aggcaaagaa guauggaagu acggagguua caacucuaua     3360

accccaagcu auuucaugcu uguagaaagu aaagacaaga aaggcaauua uaucagaacc     3420

auugaaacca ugccacucua cagagagaaa gaguucagca aaaacgaaga guuccuucua     3480

aauuauugca aagaaguaca caagcuaaaa gagcccaaaa ucauccucaa augcauaaag     3540

aaaaaugcgc uacugauagu ugauggauuu ccaaugcacc ugaaaggauc aacuggguua     3600

caacuaaaac ugcaaggcgc cguacaacua auccuugagu cagauauggu gauauaccuc     3660

aagaaacuaa ugaaguuugu gaaugaaaau aacauaaaca aaaauaaacu agaaguaaca     3720

ccauauaugg aacuauccgc aaaagaaaac auuaaacuau acgacauauu cauccaaaaa     3780

cuaacuacag gcaucuauag uaaaagaccc gccaacccag uagaaaaacu aauuaacgca     3840

cgagacauuu uuauagaauu aaccguaaaa gaacaaugca ucguacuaag ugaaauucua     3900

caccuuuucc aaugcaaacc uuuaaccuca gcaaacuuag uaucaauaaa ccucaagaaa     3960

acagugggaa uaauacaaau agccaaaaau aucacagcca aaaauguaaa acuugugcac     4020

caaucaccaa caggaauaua ugaauauaca guagacuuaa acaaaauuug a              4071


<210>  5368
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5368
augacaaaaa aggaacaacc auacaacaua ggacucgaua uugggaccuc uucaguaggu       60

ugggcaguca ccaacgacaa cuaugaccuc cucaauauaa aaaaaaaaaa ucuauggggc      120

guaagguuau ucgaagaagc acaaaccgcu aaagaaacca gauuaaauag aagcacuaga      180

cgccgauacc gaagaagaaa aaacagaauu aauugguuaa augaaauauu uucggaagaa      240

cuagcaaaaa ccgauccuuc cuuccucauu agacuacaaa acucaugggu aaguaaaaaa      300

gauccagauc gaaaaagaga caaauauaac cuauucauag acggcccaua uaccgauaaa      360

gaauacuauc gagaauuucc aaccaucuuc caccuacgaa aagaacuaau acuuaauaaa      420

gacaaagcug auauacgacu caucuaccug gcacuccaca acauacuaaa auacagagga      480

aacuuuacau acgagcauca aaaauucaac auaucuaauc uaaacaauaa ccuaucgaaa      540

gaauuaaucg aacuaaacca gcaauuaauu aaauacgaua ucuccuuccc ggaugacugc      600

gacuggaacc acauauccga cauauuaaua ggacgcggca augccaccca aaaaucauca      660

aacaucuuaa aagauuucac acuggacaaa gaaacaaaaa aauuacuaaa agaggugaua      720

aacuuaaucc uuggaaaugu cgcacaucua aacacaaucu uuaagacauc ccuaacgaaa      780

gaugaagaga aacuuaacuu uuccggaaaa gacaucgaau caaaacucga cgaucuagac      840

ucaauccuag augaugauca auucacaguc cuagaugcug ccaaucguau auauucaaca      900

aucacauuaa augaaauucu aaacggggaa agcuauuuca gcauggcuaa gguuaaccaa      960

uacgaaaacc acgcaauuga ccuaugcaaa cuucgagaua uguggcacac uacaaagaac     1020

gaagaagcag uagaacaauc aagacaagca uaugacgauu acauaaauaa gcccaaauac     1080

gguacuaagg aauuguacac aagucuaaaa aaauuucuua aaguggcuuu gccaaccaau     1140

uuagcaaaag aagcggaaga aaaaauaagc aaagggacau acuuagucaa accacguaau     1200

uccgaaaaug gaguagugcc auaucaacuu aacaaaauag aaauggaaaa aaucauugac     1260

aaucaaagcc aauacuaccc guuuuugaaa gaaaauaaag aaaaacuucu aucaauucua     1320

ucauucagaa uuccauacua cgucggacca cuacagucag ccgaaaaaaa ucccuucgcc     1380

uggauggaac gaaaaucaaa cggccaugcu cggcccugga acuuugacga aauaguagac     1440

agagaaaaau caucaaacaa auuuauccga agaaugacag uaacagauuc uuacuuaguc     1500

ggugaaccag uccuccccaa aaauucucua aucuaucaac guuaugaggu uuuaaacgaa     1560

cucaacaaca ucagaauaac agaaaaccua aaaacaaacc cuauagguuc acgacuaacu     1620

guggaaacua aacaaagaau uuacaaugaa cucuucaaaa aauauaaaaa aguaacuguu     1680

aaaaagcuaa caaaaugguu gaucgcacaa ggcuacuaca aaaaccccau ucucauagga     1740

cuaucccaaa aggacgaauu caauucgaca cuuaccacau acuuagacau gaaaaaaauu     1800

uuuggcaguu ccuucaugga agacaacaaa aacuacgauc aaaucgaaga acuaauugaa     1860

uggcuaacca uauucgaaga uaaacaaaua cuaaacgaaa aacuacacuc aaguaaauau     1920

uccuacaccc ccgaucaaau aaaaaaaaua ucuaauauga gauacaaagg cugggguaga     1980

uuauccaaaa aaauacuaau ggacaucacc acugagacua acacacccca acuauuacag     2040

cuaaguaacu acucaauauu agacuuaaug ugggccacga auaacaacuu cauuucuaua     2100

auguccaacg acaaauacga uuucaaaaac uauaucgaga accacaaccu caauaaaaac     2160

gaagaccaaa acauaagcga cuuaguaaau gacauacaug uauccccagc acugaaaaga     2220

ggaauaacgc aaagcauaaa aauaguacaa gaaauaguaa aauuuauggg ccacgcaccc     2280

aaacauaucu ucauugaagu uaccagggaa acuaaaaaau ccgaaauaac aaccuccaga     2340

gaaaaaagaa uaaaacgccu gcaguccaag cuacugaaca aagccaacga cuucaagcca     2400

caacugagag aauauuuagu uccaaacaag aagauacaag aagaacuaaa aaaacauaaa     2460

aacgacuuaa gcagcgaaag aauaaugcug uacuucuugc aaaacggaaa gucucuuuau     2520

uccgaagaau cuuuaaauau aaacaaacuc ucugacuauc aagucgacca cauccugcca     2580

cgaaccuaua uuccagacga cucacuagaa aauaaagccu uaguauuagc aaaggaaaac     2640

caacgaaagg cggacgaccu acuccuaaac ucuaacguca uugacagaaa ucucgaaaga     2700

uggaccuaca ugcuaaacaa caacaugauu gggcuaaaaa aauuuaaaaa ccuuacuagg     2760

cgaguaauca cugacaaaga uaaacuagga uucauccauc gucagcuggu acaaacgucc     2820

caaaugguca aaggaguagc uaauauucuc gacaauaugu acaagaacca aggaaccacu     2880

ugcauucaag cccgugcuaa ccuaagcacu gccuuuagaa aagcccuauc cggacaagac     2940

gacaccuacc acuucaaaca cccagaauua gucaaaaaca gaaauguuaa cgacuuucau     3000

cacgcccaag augcauaucu agcaucauuc cuaggaacau acagauuaag aagauuucca     3060

acgaaugaaa uguugcucau gaacggcgaa uacaacaaau ucuacggaca ggugaaagag     3120

cuuuauucca aaaaaaaaaa acucccugau ucucgaaaaa acggauuuau aauauccccg     3180

cuaguaaacg gcacgaccca auacgaccgu aacacuggcg aaaucauaug gaauguagga     3240

uucagagaca aaauucuaaa gauauucaac uaucaucaau guaaugugac acguaaaaca     3300

gaaauaaaaa ccggccaauu uuacgaucaa acaauuuacu cucccaaaaa cccaaaauac     3360

aaaaaacuua ucgcacaaaa gaaagacaug gauccaaaca uauacggugg auucuccgga     3420

gauaacaaau ccaguauaac caucguaaaa auagauaaca auaaaaucaa accaguugca     3480

auaccaauaa gacuaauaaa ugaccugaaa gacaaaaaaa cacuacaaaa cuggcuagag     3540

gaaaacguaa aacacaaaaa aaguauucaa aucauaaaaa acaauguacc aauaggacaa     3600

auaaucuacu caaaaaaagu ugggcuacug ucucucaauu cagaccgaga gguagcaaac     3660

agacaacaac ugauacuacc uccagagcau uccgcucuau uacgacuauu gcaaauuccc     3720

gacgaagacc ucgaccaaau acucgccuuc uacgauaaaa auauucucgu agaaauccua     3780

caagaacuaa uaacaaaaau gaaaaaauuc uaccccuucu acaaaggcga acgagaauuc     3840

cuuauagcca acaucgaaaa uuucaaucaa gcaacuacuu cagaaaaggu uaacucauua     3900

gaagaacuca uaacccucuu acacgcuaau agcaccucgg cacaucuaau auucaacaau     3960

auugaaaaaa aagcuuucgg aagaaaaaca cauggauuaa cccuaaauaa uacagacuuu     4020

aucuaccaau caguaacagg ucucuacgaa acucggaucc auauagaaug a              4071


<210>  5369
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5369
augacaaaaa aagaacagcc auacaauauc ggccuagaca ucgguacugg aucaguagga       60

ugggccguaa caaaugauaa uuaugaucua uuaaauauca aaaaaaaaaa uuuaugggga      120

guacgauuau ucgaaggcgc acaaaccgca aaagaaacca gacuaaacag aucaacuaga      180

agacgcuauc gaagacguaa aaacagaauc aacuggcuaa augaaauuuu uucagaggaa      240

cuagccaaua cagacccaag cuucuuaauu cgucugcaaa acaguugggu cuccaaaaaa      300

gacccagaca gaaaaagaga caaauacaac cuauuuauug acaauccuua uacagauaaa      360

gaauauuaua gagaauuucc aacaauauuc caccuaagga aagaacucau aauaaauaag      420

aauaaagcag auauaagacu cgucuaucua gcacuccaca acauccuuaa auacagaggu      480

aauuuuacgu acgaacauca aaaauuuaau aucucgacuc uaaauucaaa ucuauccaaa      540

gagcucauag aacuaaauca acaacuaaua aaguacgaca ucagcuuccc agacaauugc      600

gacuggaacc auauaucaga cauacucauu ggaagaggua augcaacaca aaaauccagu      660

aacaucuuaa auaacuucac ucuagacaaa gaaacuaaaa aauuacuaaa agaaguuaua      720

aaucuaauac ucggaaaugu agcacacuua aacacaaucu uuaaaacaag ucugacuaag      780

gacgaagaaa aauuaucauu uuccgguaaa gacaucgaau caaaauuaga ugacuuagac      840

ucaauacugg acgacgauca auuuaccgua cuagauacug caaaucgaau auacucaaca      900

auaacauuga augaaaucuu gaacggagag ucauauuucu ccauggcuaa agucaaucaa      960

uacgaaaacc augcaauaga ccuauguaaa uuacgcgaua uguggcacac aacaaaaaau     1020

gaaaaggcag uagguuuauc aagacaagcu uacgacgacu auauaaauaa accgaaauau     1080

ggaacaaaag aacuauauac aucccucaaa aaauuucuua agguugcacu ccccacaaac     1140

cucgcaaaag aagcagaaga aaaaauuuca aaagguacau auuuaguuaa accacgaaac     1200

uccgaaaaug gcguuguccc auaccaauua aauaaaauug aaauggaaaa aauaauagau     1260

aaccaaucuc aauauuaucc cuuccuaaaa gaaaauaaag aaaaacucuu aucaauacuu     1320

uccuucagaa uuccauacua uguaggacca cuccaaaguu cagaaaaaaa cccauuugcu     1380

uggauggaac guaagucaaa uggucaugcu cgucccugga acuucgacga aaucguagac     1440

cgagaaaaaa gcucuaauaa auucauacgc cgaaugacug uaacugauag uuaucuuguc     1500

ggggaacccg uacucccuaa aaacucguua aucuaccaaa gauaugaagu acuaaacgaa     1560

cuaaacaaua uacgaaucac agaaaacuua aaaacaaauc caacugguag uaggcuaacc     1620

gucgaaacaa aacagcauau auacaaugaa cuauuuaaaa acuacaagaa aaucacagug     1680

aaaaaacuua caaaauggcu aauagcccaa ggauauuaca aaaauccgau ucuaauaggu     1740

uuaagccaaa aagaugaauu caauucuacu cuaacgaccu acuuagacau gaaaaagauc     1800

uucggaaguu cuuucaugga aaacaacaag aauuacaacc aaauagaaga acucauagaa     1860

uggcuuacaa uauucgaaga caaacaaaua cuuaacgaaa aacuucacuc aucaaauuau     1920

ucauacaccu cagaccaaau aaaaaagaua agcaauauga gauacaaagg guggggaaga     1980

cuaucaaaaa aaauauuaac cuguaucacc acagaaacaa acacacccaa aucccuacaa     2040

cuaucaaacu acucaguucu ggaccugaug uggacaacua acaacaacuu cauaucaauc     2100

aucucaaacg acaaauauga uuucaaaaau uauauugaaa aucauaaccu aaauaaaaau     2160

gaagaccaga auauuagcaa uuuaguaaau gacauccaug ucucuccagc cuuaaaacga     2220

ggaaucacac aaucaauaaa aaucguucaa gaaauaguaa aauuuauggg ucacgcacca     2280

aaauauauau uuauagaagu caccagagaa acaaaaaaau ccgaaaucac cacuucaaga     2340

gggaaaagaa uccaaagacu acaauccaaa uuacucaaua aggcaaacgg uuuuaaaccu     2400

caacuuagaa aauacuuagu acccaaugag aaaauucaag aagaacuaaa gaagcauaaa     2460

aacgaccuca gcucagaacg uauaauguua uacuuccuac aaaauggaaa aucccucuac     2520

ucagaagaga gucuuaacau aaauaaacua ucggacuauc aaguagauca cauccuacca     2580

cgaacauaca uaccugacga cagccuggaa aacaaggcuu uaguuuuagc caaggaaaau     2640

caacgcaaag cagacgaucu ccugcucaac ucaaauguaa uagauaaaaa ucuugaacgu     2700

uggacuuaua ugcuaaacaa caacaugaug ggucucaaaa aauucaaaaa ccucacucga     2760

agaguaauaa cagacaaaga uaaauuagga uucauacauc gccaacucgu acaaaccucu     2820

caaaugguua aaggagucgc caacaucuua aacucaaugu acaaaaacca aggaacaacc     2880

ugcauacaag ccagagcuaa ucucucaaca gcuuuccgaa aagcacucag ugaucaagac     2940

gacagauauc auuucaaaca uccugagcua guaaaaaauc gaaauauaaa ugauuuccau     3000

cacgcccaag acgccuaucu agcaucauuc cuaggaacuu aucgauuacg ucgcuuccca     3060

acagacgaaa uguuacucau gaacggcgaa uacaacaaau uuuauggcca aguaaaagaa     3120

cucuacucca aaaaaaaaaa gcuaccggac ucacgcaaaa acggcuucau aauaagucca     3180

cuaguuaaug gaacuaccca auaugauaga aauaccgggg agauuaucug gaauguagga     3240

uuucgcgaca agauacuaaa aauauucaac uaccaucaau gcaacgucac acguaagacc     3300

gaaauaaaaa cagggcaauu cuacgaucaa acaauuuauu caccgaaaaa ucccaaguau     3360

aaaaaacuga uugcacaaaa aaaagacaug gacccuaaca uauacggagg auuuucgggu     3420

gacaacaagu ccaguaucac cauaguaaag auagacaaua acaaaauaaa acccguagca     3480

auuccaauaa gacuaauuaa ugaccuaaag gauaaaaaga ccuuacaaaa uuggcuugaa     3540

gagaacguua aacacaaaaa aagcauccaa auaaucaaaa acaacguccc aauaggacaa     3600

auaaucuauu caaagaaagu aggacuuuua ucucuaaacu cagaccgcga aauagcuaac     3660

cgacaacaac uaauauuacc cccggaacac ucagcacugc uacgcaucuu acaaauuccu     3720

gacgaagacc cugaccagau acuagcuuuc uaugacaaaa acauacuagu ugagauacua     3780

caagaauuaa ucacaaaaau gaaaaaguuc uauccauucu acaaaaauga acaagaauuu     3840

cuagcaucca acauugaaaa cuuuaaccaa gcaaccacau cagaaaaaau aaacucacua     3900

gaagaauuaa uaacauuacu ucacgcgaau ucuacuucag cacaccucau cuucaauaac     3960

auagaaaaaa aggcauucgg gagaaaaaca cacggauuaa cauugaauga uacugacuuu     4020

aucuaccaau cuguaaccgg ccuauaugaa accagaauuc auauagaaug a              4071


<210>  5370
<211>  4071
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5370
augagcaaga aagacagcga auacuacaua ggauuagaca uaggaacaaa cucagucggc       60

ugggcuguua caaauggcaa auacgaccua cuuaauauca aaaagaaaaa ucucuggggc      120

guucgucuau ucgaagccgc agaaagugcu gccggccgcc ggauaaaucg cucaacacga      180

agacgcuacc gcagacgccg aaauagaauu aauugguuaa acgaaaucuu cgcagacgaa      240

cuaaguaaag uggauccuuc uuuccuuacg cgccucaacu caucauggau aaguaaaaag      300

gauuccucca gaacgcguga uaaauauaau cuauuuaugg augccgacua cacagacaaa      360

aauuaccacc acaaauaccc uacaauauac caccuucgcc gggaucucau guucgacgac      420

aaacaauucg acauaagacu cgucuaccua gcccuccaca auauaauaaa auacagaggu      480

aacuuuacau acgaucaucg aaaauucaau guaucagaaa ugucaaguaa ucuuacaaaa      540

gcccucgaaa acuuuaauca agaauuaaug gaguucgaag acgcauuccc agacaauacu      600

gacuacucag cuauuucaga agucuugcua aaauccaaua acguaucaac aaaaauagac      660

gaaauaaucu cuaauuuaga acuugauaaa gauaccaaga aauaccuuaa agaagugcug      720

aaauuaauac uaggaaauag cgcuaaucuc aauacuauau uccacacuuc ucucgauaaa      780

gaugacgaaa aacuuuccuu aagcgcaaaa gacaucgaau ugaaacucaa caauuuagac      840

ucauuauuau cagacgaaca aauacacuuc auaucaucag cuaaugaacu auacucauca      900

aucacucuca acgacauacu agacgggaaa acguaccuau cucuggcaaa aauuaaacaa      960

uacgacaccc auugcucaga ucuccaagaa cuuaaagaaa ugugguauaa aacaacaaac     1020

ccagaccuca uaaagaaauc uagaaaagcc uacaaagaau acguacaugg aucauauaau     1080

acagaagauu ucuauaaagc aauauccccu uuucucaaaa uagccauacc ugaagaccuc     1140

gccaaagacg cacugaagaa aauagacgcc aauaccuauc uuccaaaaca acgcacuuca     1200

aauaauggag ucauacccuu ccaauuaaau cuuauagaac uagagaaaau aauagauaau     1260

caaucuaaau auuaucccuu uuuaaaagaa aaucgggaga aaauaaucag uauacucucc     1320

uuucgcauac cauacuacgu aggacccauc caagcaccaa ccgauaaaaa cuucucuugg     1380

augguaaaga aaauccaagc caaagcucgc cccuggaauu uugacgaagu aauagaucga     1440

gaaaaaucua guaacaacuu uauaaaacga augaccacga ccgauacaua ccuaguaggc     1500

gaaccaguac uaccgaaaaa cuccuuaaua uaccaaaaau ucgaaguacu aaacgaacua     1560

aacaacauaa gaauaucuaa aaaccuuagu caagauccac uuggaaguaa acucucugua     1620

gaagugaaac aacaaauauu cgaagaauua uuuaaacguu acaaaacagu aaaaucugac     1680

caacuaaaga aauggcugau aagaaacucu uacuuuacag aucccacaau uauaggccuc     1740

ucuaaacccu cagaauuuaa cuccucacua uccacuuuca uagauaugaa agcaauauuc     1800

ggaauggauu ucgucaacaa cccacgcaac agcagucaac ucgaagaacu agcagaaugg     1860

uuaacaguuu uugaagacaa acaaauccua ucagagaaaa uaagacauuc aaacuaucca     1920

uauagcgacc aacaaaucag aaaaauaagc aacaugagau acagaggcug gggacguaua     1980

agcaagaaac uacuguguga caucaaaguc gaaaguacga uauauaaauc acaucaacuu     2040

caucccuauu caauccuaga ccuaaugugg ucaacaaaua acaacuuuua cuccauacuu     2100

cauaaagaca aauacaacuu ugaaucaauc cuugaagauu auaaucuagg gacagacgau     2160

aaaacagaua uaaacgaguu aguagacaac auacacacau caccagcacu aaaacggggc     2220

aucauccaaa guauaaacau aguaaaagag cuagucaaau ucaugggaca cgccccucaa     2280

cauauuuuuc uggaguucac aagagaagac aaaacuucaa cacuaucaga aaguaggaag     2340

aaacgacuca agaaacucua caacaaucug ucugaagaca cuaagaaauu uaaacuagac     2400

cuaagagauu accucguacc ugauaaauca auucaaaaag aguuagaauc acaugauaaa     2460

gaccuaucaa aagaaagacu caugcucuac uuuuuacaaa augguaaauc auuauauucc     2520

gacgaagcau uagauaucaa uaggcuuucg gaauaccaag uagaccauau ucucccgcgc     2580

acuuacauaa aagaugacuc acuagaaaac aaagccuuag uacuagccaa agaaaaucaa     2640

cggaaagcag acgaccuauu auuacaaccc ucaauaauaa acaaaaacau ggaaagaugg     2700

auauauauga aagaucaugg aaugaucuca ccaaagaaau uuaaaaaccu aacacgcacu     2760

acuauaacag aagacgacaa aguacauuuu auuaaccgac agcuaguaca aacaucgcaa     2820

auaaucaaaa acguagccaa uauacuagac aacauguaca aagaacaagg uacaaccugc     2880

gucgaaacca aagcaaaccu uucaucggcc uuuagaacag ccuuuagcga acaagacgac     2940

aaauaccacu uuaaacaccc ugaauuuauu aaaaacagag augugaauga cuuccaccac     3000

gcucaagaug cauaccuggc augucuauua gguuuauaca gaauccaacg cuucccauca     3060

gacgaaaugc uacugauaaa gaaagaauau agaaaauuuu ucgcacaagc acaaaacuca     3120

guaaagaacc acggaaagau uccagacucu caaaagaaug gauucauacu cgcaccccua     3180

guaaauggug aaacgcugau aaacaauaaa acaauggaaa ccauauggaa uauggacuau     3240

aaacguaaag ucauaaaaau uuuuaauuau aaacaauaua acaucaccaa aagaacagaa     3300

aucaaaacag gagaguucua caaacaaacc uuauacagcc cccacgauga aaauguuaaa     3360

aauccuauuc cacaaaagaa aaaucuagac cccaaaaucu acggagguua cagcggcgua     3420

caaucaaguu aucuggccau aaucaaaguc gaccaaaaga aacccacuau gguuucaguc     3480

ccuauacgau ucucgaaaau gaucgacgau ggaaaacuau cccuccaauc augguuagaa     3540

aauaacauaa aacacaagaa aacaauagaa guacucaaaa ccaaaauccc cauuggucaa     3600

uuaacauaca acccgaaaaa cgguuaucuu ucaguacaau cugaaaaaga gaucgcuaau     3660

gcgcaacaac ucuuccuacc uuucgaacac guugcucucc uaaccuuacu ccaugcgcca     3720

acuucugaau acgaccaaau ucuauccuuu uaugacacag auauccuccu ugaaauauuu     3780

gaugaaauua caaauaaaau aaaaacauuc uuccccuuuu auauagauga gcaagaauac     3840

uuagucucaa uucgggaaaa cuuccaaucc gccaccguua uagaaaaagu caacgucuua     3900

caacaauuac uaauaauguu acaugcuaau ucuucaagaa uaucgauaaa acucggagaa     3960

guuagcaaaa aggaucucgg aagaaagaag aaacgaauac cacuugaaaa cacugaucua     4020

gucuaucaau cagucacugg acuauaucaa acaaaauuuc acauugauug a              4071


<210>  5371
<211>  4074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5371
augaacgacc gcaacaaaaa auacaauauc ggacucgaca uaggaacugg cuccguaggu       60

ugggcuguaa cggauaacaa cuacaauuug cugcacgcaa aaaagaagaa ccuuuggggc      120

auucgauuau ucgauggagc agaaaccgca gcauccagac gaacguuccg uuccacacgc      180

cgccguuaca gaagaagaag aaauagaauc aauugguuaa acgaaauuuu ugccgaugaa      240

uuagccaaga aagaccaauc auucuuagua agaaugaacu caagcugggu aucaaaaaaa      300

gacaaaauuc gacaaagaga ccaauacaau uuauuuaugg acucagacua cacagacguc      360

gaauaucacc gugaauuccc aaccauauuu caccuaagaa aacgucuaau ugaagauaaa      420

ucccaagcag acauucgucu ugucuaucua gcacuccaca acauaauaaa auaucgugga      480

aauuucacuu acgaacacca gaacuuugac guaucacaca uggacagcgg ccucgcacua      540

cuccuaaauc aacucaacca acaauuacag gcauuugaag uugcguuucc ugacgaaacc      600

aauuuugaau caaugucuac aauccuacua gaaaaaaaga ccccaaccca aaaaauagau      660

gagaucgucg aucaaauuca accagagaaa gacuuacuac aaauuacuaa aaacauaaug      720

aaaauguuac uaggaaauaa agcagaucuu guaaaaaucu uuucucucga uagcgaugau      780

aaaauaacug uagaauucuc uagcaacuca auugagcaag aucuaagcaa ccucgaagaa      840

acacuaucug acgaacaauu uaauauaauc acucuagcaa auucuaucua uagcucaaua      900

guacuaaacg auauucuauc uggcgaaaca uaccucucgu uugcaaaagc uagacaauac      960

ucagaccaca aaaaagauuu agccaaacua aagacuaugu ggagagaaag cccggauaga     1020

aaagcaguug gcaaagcgaa aaaagcguac gaugacuauu uacacucggg aaaauaugga     1080

aucgaagacu ucuauaaaga caugucuaaa uuucuagaaa cagcaacacc agucgaauua     1140

gcaaaagagg caaaugaaaa aauacuaaac aaaucauauu ugcuaaaaca acgaaacaac     1200

gaaaacggag ugaucccauu ucaauuaaau aaaaaugaga ugauugaaau uaucaauaac     1260

caagcaaaau acuaucccuu ccucgccgaa aauaaggaca aauuacuguc uauauuaucg     1320

uucagaaucc cauauuaugu cggaccccuc caaucaaaaa aagacaaccc auucgcaugg     1380

auggaaaaaa aaucucaagg aaaaauccga ccauggaacu ucgaugaaau uguagauaga     1440

gaagcaucau ccaauaauuu uauuagacga augacaucaa cagauacaua ucuaaucgga     1500

gaacccgucu uaccucuaaa aagcccaaua uaccaaaaau acgaaguucu aaacgaauua     1560

uccaacguac ggauaaacgc agaugaagua gacaauccuc uaggagacaa acuauccgua     1620

gauuuaaaac aagccauaua uaacgaacua uuucuaaaac auagaagugu aagcaaagcu     1680

cuacuuacca aauggcucau caaccaaucc uacuuccaaa auccgcaaau aucuggauua     1740

gcagacagca aaaaauucaa uucaggacua accacuuacc acgauuucaa aaagauauuc     1800

ggagaagacu ucgucaacga ccccacaaac caaagccaac ucgaacaacu ugccgaaugg     1860

cuaacaauau uugaagacaa aaaaaucuua aaaauaaaac uagacgauag ccaauucaau     1920

ugcacacccg aacaaauaaa aaaaauugca gguaugagau accaaggaug gggucgcuua     1980

ucaaaaaaac uccucugcga uauaaaaacc acaacaguga ccuccaaaaa ucaucaacaa     2040

gcuucuuacu caauauuaga ucuaaugugg aacaccaauc gaaauuucau auccguccuc     2100

aagaacgaua cauauaaauu ugaagauaaa auacaaacuu ucaaccugga caaaaaugaa     2160

gcggccacac cacaagcuau gauagaugaa uuacaaacaa gcccagcgcu aaaaagggga     2220

aucagacaaa gcguaacuau aauacaagaa cuugucaaau uuaugggaca cgcaccagaa     2280

cacauauuuc ucgaauucac uagagaagac gacguaaguu cauuaaccaa aagccgagac     2340

ucccgauuaa auaaauuaua ugcacgaaua gcuaaaaaca cgaaaaaaac ugacgacgag     2400

cugaaaaagu cauuacuacc uucauccgac auuaaagcca cauuaaaagc aaacaaaaac     2460

aaucuuucaa augacagacu aauguuauac uauuuacaaa ugggcaaauc acuauacagc     2520

aaccaaccuu uagacaucaa ccgucucagu gaauaccaaa ucgaucauau acuuccccaa     2580

ucauauauaa aagacaacuc auuagagaau agagcacuug uacucgcauc ugaaaaucaa     2640

cacaagucaa gugaccuacu acuuaaacca gaaauaauaa accaaaacau ugcucgaugg     2700

cguuacauga aggacgccgg ccuaauggga ccuaaaaaau ucaaaaauuu aacaaggaua     2760

caaauuacag auucagacca aaauaauuuu aucaacagac aauuagugca aacuucacaa     2820

auaauaaaac auguauccaa cauauuagau ucuauguacg gagaccaagg aacucaaugc     2880

guagaaacua gagcccaucu aucaacagaa uuuagaaaag ccuuuuccaa ugaagacgau     2940

aacuaucacu uccaacaucc cgaauucgua aaaaacagaa acauaaacga cuaccaccac     3000

gcucaagaug cauaccuagc augucuauug ggacucuacc aaaugaaaaa auauccgacc     3060

gacaacauga uucuguuaaa aaaagaauac aagagauuuu uuaguaauac aaaaaaaaua     3120

uacgacaaaa agcaucauuu ccccgacuau guaaaaaaug gauggaucau uggcucuaug     3180

uucaacggag aaaaguacgu cgacgaagag acaggacaaa ucaucuggga ccaacaaacc     3240

aaagacaugg uaucuuguau uuuucaauau aaacaauaca acgucacaaa acgcacagaa     3300

gcugcaaacg gagaauucua caaucaaacu guauauaaaa aagaccccaa cgaaaaaaga     3360

aagcucauaa gccuuaaaca aggccucgau acaaaauuau acggaggcua uaguggugac     3420

aacccggcau acaucguacu ugucaaaauc gaugacaaaa aaaacaaauu aguaaaaaug     3480

ccaauucgac uagcggaaca aguugacaac aaaacaauag acuuacaaac uuggcuugaa     3540

gagaaugucc cucauaaaaa aggaauacaa guacuuaaga ccaaaauucc ccuaggacaa     3600

gucauauaua guaaaaaaaa cggauaccuc gcacuaaaau ccgauacaga gauagucaac     3660

gcacaacaac uaguucuccc auacggcuac guugcccucu uaacccuguu acaaaaaaug     3720

ccagaaaaca aguacgacga gguguuagcc uguuaugaug acggaauccu ggacaacauc     3780

uuuguagcaa uuauugacaa aaugaaacau uuuuauccuu auuacagcgg cgaacuaaaa     3840

acacuaagcg auaauauaga uaauuucaaa gaaucaccug acguaaacaa gauaaaaaca     3900

cuccaacaac uacuucuacu ccuccacgcc aguucaacaa cagcgucgau cgaauuuaac     3960

aauguuaaaa aaaacuccuu agguagaaaa ccuaauucaa uaaauauaca aaauacagau     4020

cuacuaagcu uuucagcuac cggauuauac gaaucucgca uccacaucga cuga           4074


<210>  5372
<211>  4074
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5372
auggcagcua aaaauuauaa uauuggccuc gacauaggaa cuagcucuau aggcugggca       60

aucguugacg auaauuacca auuaaugcau gcuaaaggaa aauacggaua cggaacuaga      120

auuuauucag aaggucaaac agcugcagac agaaggggau ucagaacaac uaggaggcga      180

cuuaaucgca gaaaauggcg acugagacua cuccaagauu ucuuugaacc auacauucuc      240

ccacaagaug aagcauucuu uauccggcaa aaacaaucua auuuaguacc acaagacacu      300

aaaaaacaau acccagaauc aguccuauuc gaugacacuu cggauaagga uuuuuauacu      360

gaguacccaa caaucuacca ccugaggcaa gcccuuauga ccgaaaaucg ccaauucgac      420

auucgagaag ucuaucucgc aauacaccac auuguuaagu acagaggaca uuuccuaaau      480

aaugccccag ucaaaaccuu uaaaucugga aaccuugacu uagccauaaa auuuaacgaa      540

cuaaacgaag aauucaaauc uguuuauccu gaucuagagu uacaauuuaa cuuagaccaa      600

auagaugaaa uaaaaaaaau acuucuggau aguacaaaaa guagauuuga cagacaaggc      660

gaagcaguaa aauuaauaua uacaguuagc gaaguaaaaa caauagaaaa auuacaaaau      720

aaaauaguaa aacaauuucu uuccggaauu uuaggcuuaa acacaagauu cgacguauua      780

acgaacaccg agagugauga uucaaugugg aaauucucau uagacucaga auccucagac      840

gaugcacuag acgaaauaau ugcccaacuu gaugaccccg ccauacaugu aguagaacuc      900

guaaacgaaa uacauaguuc uauuacccua gcagguauag uuccagaggg ucaaggucua      960

ucagagagca ugguagaaaa auacgaagca cacaaagcuc accuccaauu acuaaaagca     1020

gucgaaaaau uaguagacaa aaaagccgcu gacaaacugg aaguugcaua cgaccaguau     1080

auaaacggaa aggacucaaa acaauucauu aaagaagacu ucuacaaagc aguacaauca     1140

gaaauuaaag ggguugcagg agaugaaguu gcugagauaa aucaacuuau agaguuagac     1200

caauucaugc caaaacaacg aacaaaagaa aacggagcaa uuccccacca gcuucaccaa     1260

caugagcucg acguaauaau agaaaaccaa aguaaauacu acgauuggcu uggcgaaaaa     1320

guaggaaacg aauacaaacu agacaaacuc guaucauucc gaguuccuua uuacguagga     1380

ccccuagugg acacagcaag uaaggaaaaa aaagacgaaa caagauucgc cuggaugguu     1440

agaaaaaacc cagaaaguac agaagcaaua acucccugga acuuugagga uauuguugac     1500

aaaaacgcau cagcugaaaa cuucauuaag aggaugacca cuaaagauaa auaccuuuua     1560

aaugaagaug uauuaccaaa augcucauua cuaacccaag acuucgaagu cuauaaugaa     1620

cugaauaaua uccgugugaa cggagaaaag uuuaccgccg aacaaaagca gcuaauccau     1680

gacgaacuau uuacaaaaca cgcaucugua ucaaucaaac aauuaaaaga uuuccuagua     1740

ucggaacuaa uguauguccg cgauaauuua aaaguugaag guuuagcaga ugaaaaaaag     1800

uuaaugagca gccuaucaac acaauuugac uaucagaaaa uaauccccga ccaaauuaac     1860

gacguaaaau accgcgaaga uauagaacaa auaauagaau ggagcacuau uuuugaagac     1920

gcagccauau uuaaaacuaa auuaaaagaa auagacuggc uaaccgauga acaacgaaac     1980

aaacuagcaa auaagagaua ccguggcugg ggaagccuau caaaaagacu auuagccugu     2040

cuuguagacg aaaacgggca acgaauaaua gauauuuuac gagauucaag ugauaacuuc     2100

augaugauag uaaacagacc ugaauuuaaa caagcaaucu uugacgaaaa cgcuaauuua     2160

acugaaacua auucaaaaaa agacauguuc gaaaugauag auaauuucua uacgucgcca     2220

caaaacaaaa aagcaauuag gcaaguacug uuagucguac aagauaucca aaaugcgaug     2280

ggcgguguag ccccacaaaa aaucuucauc gaauucgcua gggaggauga aguaaacggc     2340

agaagaacaa acucaagaua uucucaaaua agagaaaugu auagcaaagu aacagaugau     2400

guuauaaaua aaacaguccg uggagaauua gaagcaaaca aaggaucacu uaauaaagaa     2460

aagcuauucu uauauuuuuu gcaaggaggc auagaucuau acucaggaaa gacaauaaac     2520

auagauaaua uaaguaguua cgaugucgau cacgucuugc cacaaaguuu uauaaaagau     2580

gauagcauaa auaauaaggu gcuaguaaau gcaaaaggua auagagucaa aaacgacaau     2640

guacccaugg aauuauuugg cgcaaagaug uauccgaccu ggaaaaggau gguagauaca     2700

ggaauaaugu ccaaggccaa auucaaaaac cuuacuuuaa accccgauaa cguggauaag     2760

uuuaaagcag agggguuuau caaccgacaa uuaguagaga caaggcaaau caucaaauua     2820

gucgcacaaa uacuaaauga uaaauuagga gaugauacaa aaauagucuc agugaaagcc     2880

aaccuaacac acgauaugcg gguauucuuu gauuucccaa agaauagaaa ugucaauaau     2940

uaccaucacg cauuugacgc cuauuuaucc gcauucguag ggacuuaucu auauaaacgc     3000

uauccuaaau uacaaccaua cuuuguauau ggagaguuca aaaaaaugga caaauuaaaa     3060

aacaugacaa guuucgacuu cuuaagagac uuaaaacaca cagacaaagu uguagacaaa     3120

gacacaggag aaauaaucgc cgacaaacua gcgguaauca caaauaugaa uaagauauau     3180

gacuuuaaga aaguauuagu aacccacgaa guccacgaaa auucaggagc cauguacgau     3240

caaacacuau acccagcuaa guuggauaaa agucuaggua gaaaaggagc caaacaacua     3300

auaccaaaaa aagacgacau gccaacggcu cucuauggag gauacacagg aaguaaggac     3360

gcauuuauga guaucguaag acuaaaaaag aaagacgaag uauauuauaa aguaauagga     3420

auaccaguaa gaauagcaga uagcguacaa cacgguaaag aagucgacgu cgaagcauua     3480

acucaauuua ucgagacgaa auuuaccaaa gaaaacuuaa acaaaaaaac aggagaaauu     3540

accagaaaag uagaaugguu cgaauuagua uuaccaaaag uacuauuugg ucaagccgua     3600

uuugacggag gacaaccauu caugcuagga agcucaacgu acccgcacaa uuuacgagaa     3660

cugguuuuaa ccaaagauuc aguaaaaaaa aucgcaggca aggaaaguaa caagucauau     3720

cuggacgccu acgacgaaau aauggaagcc augaauaaau acuuuccccu cuaugauaca     3780

aauaaauuua gagaaaaagu cauagaaggu cgaucuaaau uuauagaucu accagauaag     3840

gaugaauaug aaaauaacaa acuuguacaa guaggaaaau caacuauaau agacagaaug     3900

uuuauaggac uacaugcaaa cgcggcuaga acagauuuaa aaaauauagg caucacuacu     3960

ccauucggau ucaugcaaca agcaaguggu auuaaacuca cuaaagacac aguugucuua     4020

uuccaaucau cuacaggacu guucgaaaga aaaguagcau uaaaggaucu auga           4074


<210>  5373
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5373
augaaagagc cuuacggagu agguuuagac auagguacaa auucuguggg auggacugua       60

guugaugcga guggucaugu cagaaaaauc aaaggacaaa caggaauugg aguaagguug      120

uuuaaagaag gggcagcggc cgcugauaga cgcgguuuca gaaccacaag aagacgucuc      180

aaaagaguua aauggagacu aagacuacua agagaauuuu ucgaucagcc aauaucaaaa      240

guagacauaa auuucuuugc aaggagaaaa uacucagacg ugucgccacg agauccuaau      300

uauaauggcc uugagaaaac cuuauuuaau gauagaucag aucaagauuu cuaccaugau      360

uauccaacua uauaccaucu acgagaagcc cuaaugacac aacauagaaa auuugacgua      420

agagaaaucu accucgccau acaucacaua gucaaauacc gaggaaauuu cuuaaggaau      480

gacgcagcca cagcuuacag aucagguaca uuagaucuac aacaacauuu cgaaaccuua      540

aaucaucugu ucucucaagc cgauuuagaa cuaaaccuca accuuacuac agacgucgca      600

uugcucgacu caauaaaaca gacuuuaguu agaacagaca uaucgagaag ugauagacaa      660

aaacuaauca ugccacuauu agcaguccua acuggugcaa caaccgcuga aaagaagcgc      720

caaaaagcag uaguaaccga guucgcuaaa gcccuagucg gaaacaaaac aaaaauagac      780

guucuaaccc uaacagauau agaugcaaca gaagccaaag acugggcuuu cucccuagaa      840

gaaaaucaag auaaguuacc aggaaucgag gaccgguucu cagaaguagg ccaacaaauu      900

auagacgaag ugauaagauu auacgccucg gucaaucuag cucaauuaau uccugaggga      960

aaacgauucu cacaaucuau gguagaaaaa uauaaaagac acggagaaga ccuuaaauua     1020

cuaaaagccu acauaagauc ccaaagcgau gcuaaacgag gacgcgcgau acgcgcaaca     1080

uaugaccaau auauagacgg cguaaaaucc aagcaaguaa cacaagaagc auuucaaaaa     1140

gcccuuggca aauuuguuga cgcagauguu gaucuaaacu caugggcagc acaaaucaaa     1200

caagcuauag auucagaaca auuuaugcca aaauuacgaa caaaacagaa cggcgcgaua     1260

cccuaucaag uccaacaaaa ugaguuagau caaauuauua guaaccaaaa agaauauuau     1320

ccaugguuag gugaagaaaa cccaguaauu aaacgaagag gaaaauuccc auauaaacuc     1380

gaugaacugu ucggauucag gguccccuau uacguaggac cauuaauaac caaggagaug     1440

caagcagcaa cugcuaacgc ggguuuugcu uggaugguac gaaaggcaga cgguccgaua     1500

acuccuugga acuucgacga caaaguagau agaguagcuu cggcaaccgc auuuauacaa     1560

agaaugcaaa cgacagacac guaccuaaua ggcgaagaag uguuaccagc aagaagucuu     1620

auauaucaaa gauuuauggu uuuaaaugaa uuaaauaaua ucagaguaga ugauggccaa     1680

uuaacaccca gacaaaaaca acgacuauac aaucaaguau uuaagaaaca caaaagaguu     1740

acagcuaaaa auauacaaga uaauuuaguu gcggcaagag aguucccaac agcuccaaga     1800

auuacugggu uagccgaucc caaagucuuc aacaguucac uaucaacuua ucacgacuua     1860

aaacaaguuc uccccgaagc aaugucagac guaaggaaac aagcagauau agagaaaauc     1920

auacucuggu caacaauuuu cgaagauucc acaauauuua aacaaaaauu aacugaaaua     1980

ucauggcuaa cagcugcuca acuuaaacaa uuaggccaac uaagauauag aggauggggu     2040

caacuauuaa gaaaacuacu aacuggucuc aaggaugaua auaauagaag uaucauggac     2100

ggacuauggg aaacaaaugc aaauuucaug caacuaagaa gucaaccugc gaucgagaac     2160

cagauuaaau cugcaaauca agcgaaucua acuacggcag accuccaaga uacuauaaac     2220

gaauuauaca ccucaccaca aaauaagaaa gcaauaagag aggucauggu cguccuggcu     2280

gauauuaaag augccaugca ugggcaaacu ccaaacugga uuuucguaga agcugcccgc     2340

gguggcggcg uugcaggaag acgaacacag agccgaucaa aacaaaucgu cgcagcauau     2400

caagggacag cacaggcaau uguucgugau caaguacaac gggaacuaaa agaaaagauc     2460

aaaaauaaag ccaauuuuaa cacucgauua guacuauacu uuuuacaaaa cggaagagau     2520

uuauauacag gugacgcuau caacauagau agacucuccg aauacgauau agaccauauu     2580

uuaccacaga gcuuaguuaa agaugacagc cuagauaaca gagucuuaac uaaugcaaga     2640

auaaaccgug agaaaaacga uacauuugcu uccgaaaaau uugguaagaa aaugggugcg     2700

aaauggaaag aaaugcauag gaugggcuua augacaaaaa gaaaacuuga ccauuuacuu     2760

augagaccag acgaaauuuc gaaauacgca acaggauuca uaaacagaca gcuaguggaa     2820

accagacaag uuauaaaauu gguagaagaa cuaauaaacg cagaauaucc aaguacagcu     2880

auuguaagcg ucaaagccaa cuuaacacac caauuccgac aaacuuuuga cuuccccaaa     2940

auacgugaag uuaacgauua ccaucaugca uucgacgcag cacuaaccgc auucaucggu     3000

acauaucuau uaaaaagaua cccaaaacua gagagauuuu ucguauaugg aaaauucgca     3060

aaacaaccgg uaaaucuuac ucgcuucaac guaauacgaa aacuagcaaa aaccgacacc     3120

ccaaucacag acauagauac aggagaagug uuaugggaca aauuaacaga uauaaaguac     3180

uuugaaacac uuuaugauua caaacgauug cuagucacuc gcgaaguauc agaaaacuau     3240

ggugcaaugu ucgaucaaac ucuauugaaa gcuagcgaca augguaaaag auuaauucgc     3300

aaaaagcaau cauucgacac cgcaauauau ggaggauaca caggaaaaac cuuagcauac     3360

auggccaucg ucaaaguguc aacuaagaaa gacguacagu accaaguagu cggcguaccc     3420

acuacaaugg ugacccaaau agaacaacug gagcagcaag guaugacaaa caaacaagcu     3480

guuacuaaag uaaucgaacc gcuuaucgcc gccaauaaca agaaaaugcu uacauaugag     3540

guacuauuac caaaaguaag auuugaacaa gucguuagag auagaauuaa aggccaaacu     3600

caucgauucg cacuuggcac agacacguac uaucacaaua uccaagaacu auaccuaccu     3660

cucacucugc aacgagcauu ucuaggcaaa caaaaugaau cagaaguuca auuaaaccaa     3720

aaccuacuua ccguauucga ugcgacccua gcgcaaguua accgauacuu cccacucuac     3780

gaaaugaaua aguuuaagaa aauacucaau agugcgagag accaaauaga acuccuagaa     3840

gcuaaaaauc aauucgucaa aaauaaauua guauuaggaa aacagggaau auuaaccaau     3900

auguuuauug gacuacaugc aaaugcuucu uacggagacu ugaaaguacu cggggugaaa     3960

auagauuucg gaaaacucca aguaccuggc ggaauaaaac uuacagggga ugcagaaaua     4020

auauaucaau cuccaacagg acuauucgaa cgaaaaauug cacuaaaaga ccucuga        4077


<210>  5374
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5374
augaacgaac cauacggagu aggccuagac auaggaacca auucuguagg auggacaguc       60

guggauauga acggcagggu uaggaaaguu aaaggaaaaa cagcucucgg agccagauug      120

uucaaagaag gagcaacagc ugaggacaga agaggauucc gaacuaccag aagaagauua      180

aaacgaguua aauggcgauu acgacuauua agagaauuuu ucgaccaacc aauaucaaaa      240

auagacccga acuucuuugc aagacgaaaa uacucagaua uaucuccaag agacccaaac      300

uacaacgguc uagcaaaaac auuauuuaau gacaggaccg aucaggaauu uuaucacgau      360

uacccaacaa uuuaccaucu acgagacaaa cuaaugacuu cagacagaaa auuugacaua      420

agagaaauuu accuagcaau acaccauaua gucaaauauc gaggaaacuu ucuccgaaca      480

ggaccagccu cucaauaugg aucugcgucc cuacaccuug cgacauccuu ucaaaaauua      540

aaugaccugu ucgcacaaag ugaagaaucu cuaaacuuca agcuaguuac agaugaagcc      600

caacuucaac aaauacaaca aauacuggua aggacagacu uaucgcgauc agaacaacaa      660

cgacaaaucu ggccacugau gguuguacua augggagcaa cagcagcaga aaagaaaaga      720

caaaaagauu ucgcuaaaga auuagcuaau gcacuugucg gucuaaaagc aaaauuaaau      780

gucguaacuu uaacaaacgu cgacacaaca guagucaaag auuggacuuu uacucuagaa      840

gaaucucaag acaaacuccc agaaauugaa gaacaauugu cugaagucgg ccaacaaaua      900

auggacgaag uaauucaacu auaugcuagc guaaauuuag cgcagcuaau accagccgga      960

aaaagauuuu cucaacauau gguugaaaaa uauaaucacc augagaaaaa ccuagaacuu     1020

cuuaaagcau auauccacuc acaaagcgau agcaagcgag guagagaaau ccgagcaaca     1080

uacgacagau acauagaugg aguaaaauca aaaccuguaa cccaagaaau guuuuauaaa     1140

gaucugauga aauacguaga agcagaugcc acuucaaauc aucucgcagc ggaaauaaaa     1200

gacgaaauag acuccgagca auuuaugcca aagcuacgga ccaaacaaaa cggaucaaua     1260

cccuaucagg uucagcaaua ugaauuagac caaauaauaa gccaucaaaa gaaauauuau     1320

ccuugguuag gugaagaaaa cccgguugcu gaacgaagag guaaauuccc auauaaacua     1380

gacgaguugg uaggauuuag gguuccauac uacguaggcc cccuaauaac caaagaagac     1440

caacaagcga caaguggugc uggcuucgca uggaugguaa gaaaagcaga cggaccaaua     1500

acaccaugga auuucgacca aaaaguugac cgcaucgcau ccgcaacggc cuuuauacaa     1560

agaaugcaaa ccacugacac auauuuaauu ggugaagacg uccugccagc ccguagucua     1620

auauaucaaa gauuuauggu auuaaaugaa uuaaauaaca ugagaguaga agaccaaaaa     1680

cuagcaccac ggcaaaaaca gcgccucuac aaccaagucu ucaaacaaca ccaacauguu     1740

agugugaaaa acauucaaca aaaucugaug gaugcaggag aauacagaga aacacccaaa     1800

aucacaggac ucgccgaccc uaaaggguuu aacucaaguu uaaguacaua ucaugauuuc     1860

aagaaaauac ucccagaagc aauagcugac gaacauaaac gcgcggauau ugagaaaaua     1920

auacuaugga gcacaacauu ugaagacucg gcuauauuua aacaaaaacu agaagaagug     1980

gcguggcuaa cagacgcaca aagaaaacaa cuaucgggac uucguuaccg cggauggggu     2040

caauuaucgc auaaacuucu aaccgcuuuu aaagacgaua acggcagauc uguaauggau     2100

ggacuuuggg aaacaucuga caauuucaug caacuacgaa aacagcccgc aauagaaaca     2160

caaauaaaag aagcaaauca agcuaaucua acagaugcgg auauacaaga uacaauaaau     2220

gaauuguaua caaguccaca aaauaagaaa gcaauaagag agguaauguu aguucuagac     2280

gauauuaaaa acgccaugca uggacaaacc ccaucaugga uauuuauaga agccgcucgc     2340

gguggaggug ucgccggcag gagaacucaa ucaaggagua aacaaauugu ugaagcauac     2400

aaaagcacag cuaaagaaau ugucucugaa aaaguccaac acgaacuuaa cgagaaaauu     2460

aaagcaaaag cggauuuuaa uacgagauua guacuauacu uccuccaaaa cggucgagac     2520

cuuuacacaa augaagcuau aaauaucgac cgacuaucag aauacgacau cgaccauauc     2580

cuaccucaga gccuaguaaa agacgacuca cuagauaacc gcguguuaac cucagcacga     2640

auaaauagag agaaaaacga uaccuucgca ucggaaaaau uuggcagaaa aaugggugcu     2700

caauggcggg aauuacacag aaauggauua augacacaaa ggaaacuaaa acauuuauua     2760

augagaccgg augaaauuuc caaacaugcc acagauuuca uuaauagaca auuaguagaa     2820

acacgacaag ucauaaaacu cguagaagaa cuaauaucau cagaguaucc cucagcauca     2880

auaguagcag ucaaggcaaa uuuaacacau caauuucgac aaacauucaa uuuuccaaaa     2940

uuaagagaag uuaacgauua ccaccaugca uuugaugccu cauuaacggc uuuuauuggu     3000

auguaucuau uaaaacaaua cccuaaauua gaaagauucu ucguauacgg aaaauucgca     3060

aaacaaccga uaaaccuuac aagguuuaau guuauucgaa aauuagcugu ggcagaggaa     3120

ccaauagcaa auauugaaac aggagaaaua cuaugggaua agaccgccga uaucaagugu     3180

uucgaaaaac ucuauaauua caaaagauua cucgucacgc acgaaguacg ugaaaauuau     3240

ggugcaaugu uugaucagac aauuuuuaag gcgacugacu caagaaagag gcuuauacag     3300

aaaaagaaag gauucgcaac ugaaacuuau ggcgguuaua caggccgaac cuuagcauau     3360

uuagccauug uuaaaguagu aacuaauaaa gauacccaau accaaguagu aggcaucccc     3420

acuacaaugg uaacacaaau acaacaacuc gaagcaucag gacuuaccuc aaaacaagcu     3480

guuacuaaag caauaggugc aaugaucaac gaaaacaaca agaaauuaca aucuuacgaa     3540

guacuucuac caaaaguaag auuugaacag guuguaaggg augaaguuaa aggacauauc     3600

caucgauuug cacuuggcac agauacauau uaccacaaua uccaagaacu auaccuaccu     3660

uuaaccauac aaagagcauu ucuaggaaaa agaaaugaca cagaugcaca aacagagcaa     3720

aaccuccuca caguguucga gacuacacua gcgcaaguca auagauacuu cccauuauau     3780

gaaaugaaca aauucaaaca gauauuaaac ucagcccaag aaaaauucgc agcuuuacca     3840

acuaaaaaca aaacaguuaa aaacaagcua guaaccggaa aagacggagu acuaaauaau     3900

uuguuuauag gccuacacgc aaaugccagc uuaggugacc uuaaaaucuu aggaaugaaa     3960

accggcuucg gcaaauuaca gguaccagga ggucucaaau uaacagauca agcagaaaua     4020

auuuaucagu cuccaaccgg auuauucgaa cgaaaagucg cccucaaaga uuuguga        4077


<210>  5375
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5375
augaacgagc ccuauggagu aggacuagac auuggaacaa acaguguagg auggacgguc       60

gucgacauga auggucgagu ccgcaaaguc aaagguaaaa cagcacuagg cgcacgacuc      120

uuuaaagaag gagccacagc cgaagaccgc agaggcuuca ggacaacuag aagaagauug      180

aaacgaguaa aauggcgccu ccgcuuacua agagaguucu uugaccaacc aauaucgaag      240

auagacccaa acuucuuugc aaggagaaaa uauagugaca ucucgccccg ggauccaaac      300

uacaauggcu uagcaaaaac acuguuuaau gaucgaacug aucaagaauu uuaccaugau      360

uacccaacua uauaucaccu uagggacaaa cuaaugaccu cagacagaaa auuugauauc      420

agagaaauau accuggcaau ccaccauaua guaaaauaca gagggaauuu ucuaagaaca      480

gggccagccu cucaguacgg aagugcguca uuacauuuag caacuagcuu ucaaaaacuu      540

aaugaccuuu ucgcacagag ugaagaauca cugaacuuua aauuagugac cgacgaagca      600

caauuacaac aaauccagca gauucuagua cgaacggauc ucucaagaag cgagcaacag      660

agacaaaucu ggccauuaau gguaguucuu augggugcaa cagcagccga gaagaaacgu      720

caaaaagacu ucgccaaaga acuagcgaac gcccuagugg gucucaaagc aaaauuaaac      780

gugguaacac uaacuaaugu ggauacaaca guaguaaaag acuggacauu cacacuagaa      840

gaaucacaag acaaacuucc agaaauagag gaacaacuau cagaaguugg acaacaaaua      900

auggaugaag uaauacaacu auacgcaucc guaaauuugg cacaacucau uccagcgggc      960

aaacgauucu cccaacacau gguagaaaaa uauaaccauc augagaaaaa uuuggaacua     1020

uuaaaggcuu auauacauag ucaauccgac uccaaaaggg guagagaaau aagagcuaca     1080

uaugacagau acauugacgg agucaaauca aaaccaguua cacaagaaau guucuauaaa     1140

gaucuaauga aauacguaga agccgacgcu acaucaaauc acuuagcugc ugaaauaaaa     1200

gacgagauag auucagaaca auucaugcca aaacugagaa caaaacaaaa uggaucaaua     1260

ccguaccagg uucaacaaua ugaacucgac caaauaauca gucaucaaaa gaaauauuac     1320

ccuuggcuag gcgaagaaaa ucccguugca gaaagacgcg gaaaauuucc uuacaaacua     1380

gaugaacugg uagguuuuag gguaccauac uacguaggac cucucauaac aaaagaagac     1440

caacaggcaa cuucuggagc cggauuugca uggaugguaa gaaaagcaga uggaccaauu     1500

acgccuugga auuucgauca gaaaguagau cgaauagcau cagcaacagc guucauccaa     1560

cgcaugcaaa cgacagauac guaucuaaua ggggaagaug uacugccggc cagaucauua     1620

auauaccagc guuucauggu ccuaaaugaa cuaaauaaua ugagaguaga agaucaaaaa     1680

uuagcaccac gacaaaaaca aagauuguac aaccaaguau ucaaacaaca ucaacauguu     1740

uccgugaaaa acauucaaca aaauuuaaug gaugcaggag aauaccggga aacaccaaaa     1800

auaacuggac uugcugaucc caaaggauuc aauagcucac uaucaacaua ucacgauuuu     1860

aagaaaaucu uaccugaagc aauagcggac gaacacaaaa gagcagacau agagaaaauc     1920

auacuauggu cuaccacguu ugaagauucc gcgauauuca aacaaaaauu agaagaagua     1980

gccugguuaa cggacgcuca acgaaaacaa uugagcggac uuagauauag agguuggggc     2040

caacuaucac auaaacuuuu gaccgcuuuu aaagacgaua auggacgauc aguaauggau     2100

ggauuauggg aaacaagcga uaacuucaug caacuccgaa aacaaccagc aauugagacu     2160

caaauaaaag aagcuaauca agcgaaucua acugaugcag auauacaaga cacuauuaac     2220

gaauuauaca ccucaccaca aaacaagaaa gccauacgug aaguuauguu agugcuagac     2280

gacauuaaaa acgcaaugca uggacaaacu ccauccugga ucuucauaga agcagcacgg     2340

gguggaggag uugcaggaag acgaacacaa agccggucaa aacaaauagu cgaagcauau     2400

aaaucaacgg caaaagaaau aguaucagaa aaaguccaac augaauuaaa cgagaaaauu     2460

aaagcaaaag cggacuuuaa uacgagauua guuuuguauu uccugcaaaa cggcagagau     2520

cucuauacaa augaagcaau aaacauugac agacuaucug aauacgauau cgaccauaua     2580

cucccccaau cuuuaguaaa agacgauuca cuagauaaua gaguauugac aagugcuagg     2640

auaaaccgag agaaaaauga uaccuucgcu agcgaaaaau ucgguagaaa aaugggugcc     2700

caguggaggg aauugcacag aaacggccuc augacacaac guaaacucaa acaccuauua     2760

augagaccug augaaauauc gaaacacgcu acagauuuua ucaauagaca acucguggaa     2820

acucgacaag uaaucaaauu aguagaagaa cuuauaucua gugaauaucc aucagcuuca     2880

auaguugcag uaaaagcaaa ucuuacgcau caguuucgac aaacauucaa cuucccaaag     2940

uuacgggaag uaaacgauua ucaccaugca uuugacgcaa gccuaacggc auuuauaggu     3000

auguaucuuu uaaaacaaua uccaaagcuc gaaagauuuu ucguauacgg aaaauucgca     3060

aaacaaccaa ucaauuuaac aagauucaau gugauuagaa aacucgccgu ugcugaagaa     3120

ccaauagcaa auaucgaaac aggggaaauc cuaugggaca aaacagccga cauuaaaugc     3180

uucgaaaaau uauauaacua uaagagacua uuagucacac augaagucag agaaaauuac     3240

ggcgcaaugu ucgaccaaac aauauuuaaa gcaaccgauu cuagaaaaag acuaauucag     3300

aaaaagaaag gcuuugccac agaaacguau ggaggauaua caggaagaac acuagcauac     3360

cuggcuauag uuaaagucgu aacaaauaaa gauacccaau aucaaguugu agguauacca     3420

acuacaaugg ucacucaaau acaacaacua gaagcaucag gcuuaacauc aaaacaagca     3480

guaacaaaag cuaucggagc aaugauaaau gaaaacaaca agaaauuaca aucguacgag     3540

guacuauuac caaaaguucg guucgaacaa guaguuagag acgaaguaaa aggacacaua     3600

cacagauucg ccuuggguac agauacuuac uaccauaaca uacaagaacu auaccuacca     3660

uugacaauac aaagggcuuu ucuaggcaag cgaaacgaua cagaugcaca aacagaacaa     3720

aacuuauuaa ccguauucga aacaacucua gcccaaguca auagauauuu uccacuauac     3780

gaaaugaaca aauuuaaaca aauacuaaau agugcccaag agaaauucgc ugcauuaccc     3840

accaaaaaca aaacaguuaa gaacaagcua guaacaggaa aagacggugu gcuaaacaau     3900

cuauucauug gacuacacgc uaacgcauca uuaggugacu uaaaaauauu agggaugaaa     3960

auuggauucg gaaaacuaca aguuccugga ggcuuaaaau uaacagauca agcagaaaua     4020

auauaucaaa guccaacagg cuuauucgaa cgaaaaguag cgcugaaaga ccuauga        4077


<210>  5376
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5376
augaaagaac cauauggggu agguuuggau auaggcacca auuccguagg cuggacagua       60

guagaugcua guggccacgu gaggaaaaua aaagggcaaa caggaauugg aguaagacua      120

uuuaaagaag gcgccgcagc ggcggauagg agagguuuca gaacaacccg aagacgccua      180

aaaagaguaa aauggaggcu gagauuauua cgagaauuuu ucgaccaacc cauaucaaaa      240

guagauauca acuuuuucgc aagacgaaaa uauagugaua uaucaccuag agacccaaac      300

uauaauggau uggagaaaac auuauucaau gauagaucag accaagacuu cuaucacgau      360

uacccgacaa uauaucauuu aagagaagcg cuaaugacgc aacauagaaa auucgaugug      420

cgcgaaauau aucuugcaau ccaccauauu guaaaauauc guggaaacuu ccuaagaaau      480

gaugcggcca cggcuuaccg aagcggaaca uuagaucuac aacaacauuu cgaaacguua      540

aaccaccuau uuucgcaagc ugaccuugaa cuaaaccuaa auuuaacaac agacguagcc      600

uuacuugauu ccauaaaaca aacguuagua cguacagaua uaaguagauc cgauaggcaa      660

aaauuaauaa ugccacuacu agccguauua accggugcga cuaccgccga aaagaaaaga      720

cagaaagcug uuguaacaga auuugcuaaa gcguuaguag gcaacaaaac uaagauagau      780

guucuaacac uuacagauau ugacgcgacc gaagcaaaag acugggcuuu cucacucgaa      840

gaaaaucaag acaaacuacc agggauagaa gacagauuuu ccgaaguagg acaacaaauc      900

auugacgaag uuauuaggcu auacgcuagc gucaaccuag cacaacucau acccgaaggc      960

aaaagauuua gucaauccau gguugaaaaa uacaaaaggc auggagaaga ccucaagcug     1020

cuuaaagcau auauaagguc ucaaucagau gccaaacgag ggagagcuau aagagcaacu     1080

uacgaccaau auaucgaugg uguaaaaucc aaacagguga cacaagaagc auuucaaaaa     1140

gccuuaggca aauucgucga ugcagaugua gaucuaaauu cuugggcagc ucagaucaaa     1200

caagcaaucg auagugaaca auucaugccu aaauuacgaa ccaaacaaaa cggagcaaua     1260

ccguaccaag ugcaacagaa cgaauuagau caaauuauaa auaaucaaaa agaauacuau     1320

ccaugguuag gagaagaaaa uccugucaua aaaagacgag gcaaauuucc uuauaaguua     1380

gaugagcuag ucggauuccg aguaccauac uauguugggc cuuuaauaac aaaagaaaug     1440

caagcugcaa cugccaaugc cgguuuugcc uggaugguca gaaaagcuga cgggccuauu     1500

acaccaugga auuuugauga caaaguagac agagucgcau cugcuacagc auuuauacag     1560

cgaaugcaaa caacugacac auaucuaaua ggagaagaag uucucccagc aagaucccua     1620

auauaccaaa gauuuauggu ucuaaacgaa uugaauaaca uaagaguaga cgacggucaa     1680

cuaacaccua gacaaaagca aagacuauac aaucaaguau uuaagaaaca caaacgggua     1740

acagcgaaaa auauacaaga caaucuagua gcugcggggg aguucccaac agccccgagg     1800

auuacgggac uagcagaccc uaaaguuuuu aauaguucac uaucaacaua ucaugauuua     1860

aaacaagucu uaccagaagc uaugagcgac guaagaaagc aagccgauau ugagaaaaua     1920

auacucuggu cuacaauuuu cgaagacagc acaauauuca aacaaaaacu uacugaaaua     1980

aguuggcuaa cagcugcaca auuaaaacaa uuaggucaau uacguuauag aggauggggu     2040

caacuaucua gaaaguuauu gacaggucuu aaagacgaua acaacagauc aauaauggau     2100

gggcuuuggg aaacaaaugc aaacuuuaug cagcuaagau cgcaaccagc aauagaaaau     2160

caaauaaaau cagcgaauca agccaaucua acaacagcag aucuucaaga cacuauaaau     2220

gaacuauaca caucaccaca aaauaagaaa gcgauuagag aagugauggu aguccuugca     2280

gauauaaaag augcuaugca ugggcaaacc cccaauugga uuuucguuga agcagccaga     2340

ggcgguggag ucgcaggaag acguacacaa ucaagaucaa agcaaaucgu ugcagccuac     2400

caaggaacag cccagguaau cguaagcgac caaguucaaa gagaacucaa agagaaaauu     2460

aaaaacaaag caaauuucaa cacaagauug gucuuauacu uuuuacagaa ugguagagau     2520

uuauacaccg gagacgccau aaacauagac cgauuaagcg aauaugacau agaccauaua     2580

uugccacaau cuuuagugaa agaugauuca cuagacaaua gaguauuaac uaacgcucga     2640

auaaacaggg agaaaaacga cacauucgcc agcgaaaaau ucgguaagaa aaugggugcg     2700

aaauggaaag aaaugcacag aaugggucuc augacgaaaa gaaaauugga ucaucuacua     2760

augaggccug augaaauaag caaauacgcg acagguuuua uuaauagaca auuaguagaa     2820

accaggcaag ucauuaaauu agucgaagaa cuaauaaaug cagaauaucc uucaacagcg     2880

auaguuuccg uuaaagcaaa ucuaacacac caauuuagac aaacuuuuga uuucccuaaa     2940

auaagagaag uuaaugauua ucaccacgca uucgaugcgg cuuuaacagc guuuauaggu     3000

acauaucugu ugaaaagaua uccaaaacuc gaaagauucu uuguguaugg aaaauuugcc     3060

aaacaaccag uaaacuuaac ccguuucaac guaauaagaa aacuggcaaa aacagacaca     3120

ccuaucacgg acauagacac aggagaagua uuaugggaca aauuaacaga uauaaaauac     3180

uucgaaaccu uauaugauua uaaaagacua cugguuacuc gagaagucag ugaaaauuac     3240

ggcgcaaugu ucgaccagac ccuacuaaaa gcaucagaua augguaagcg gcuaauuaga     3300

aagaaacaga gcuuugacac agcaauauac gggggcuaua caggcaaaac ucuggcauac     3360

auggcaauag uaaaaguguc aacuaagaaa gacguacaau aucaaguagu cggaguaccu     3420

acaacaaugg ucacucaaau agaacaacuc gaacaacaag gaaugacuaa caaacaagcc     3480

guaacaaaag uaauugaacc acuaaucgca gcgaauaaua agaaaaugcu cacauaugaa     3540

guucuccucc ccaaaguucg auuugaacaa gucguaagag auagaauaaa aggacaaacc     3600

cauagauucg cucuaggaac agauacauac uaucauaaca uccaagaacu uuaucuacca     3660

cuaacauuac aaagagcauu ucugggaaaa caaaaugaaa gcgaaguaca acuaaaucag     3720

aacuugcuua ccguauuuga ugcuacacuc gcacaaguaa acagauacuu ucccuuguau     3780

gagaugaaca aauuuaagaa aauauuaaau ucagcacgcg aucaaauuga acuucuagag     3840

gcgaaaaacc aguuugugaa aaauaaauua guacuaggaa aacaggguau ccuuaccaau     3900

auguucauag gacugcaugc aaacgcgagc uucggcgacc uuaaaagucu cggaguaaaa     3960

acaucauuug gccaacuaca aguaccaggc ggaauaaaac uaacgggaga cgcagagauu     4020

auuuaucaau caccuacagg acuauuugaa agaaaaauag cacucaaaga ucucuga        4077


<210>  5377
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5377
augaaacaag gaaaaagggg auacuacuua gguuuagaua uaggaacaga cucaaucggc       60

uacgcgguca cagacgaaca auaccacuug cuuagauucc acggaaauga ugcauggggu      120

agccauguau ucgacucagc aucacuuugc gacgaaaggc gaggauuccg uagcgcuagg      180

agaagacuag accguagaca acaaagagua aaacuccuac aagaaauuuu cgcaaaagaa      240

auagccaaua ucgauccaag auucuuuauu agacucucag aauccuaccg auggcgcgac      300

gauaccgacg aucgcuacau auauuucaac gaagaggaau acacagauau acaauacaug      360

cgagauuacc ccacaaucca ucaucuuaua ugugaacuca uggagaauga ggaagaacac      420

gauauaagac ucguauaucu agccugcgca uggcuaguug cacaucgcgg ucacuuuuug      480

ucacaauuaa guguagauaa uauagauuca uuaauagaua uagaauccgu cuaccaaaaa      540

uuucuacacu uuuucauaga uaacggaugc gaaaaucccu ggauccagga ugcagaauug      600

guugggggua uccuaaaaga acauauagga guuacagaaa aacauaaucg acuaggagaa      660

auaauguugg guaauagaaa accaaguaaa gauguaagag auaccuuuuu auauucagaa      720

uacuguauca uuagacuauu agcuggaggg acuugcaaau uaagggauuu auaugguaaa      780

gaagaauacg aagacuuugg cucaguauca cuagaaaugg augacgauaa acuagcagaa      840

auaaugauca acauuggaga agauuacgaa cuuauagcag uacuaagauc acugcacgac      900

uggucaguac uugccgauau acucgacgga caaaaugguc uuacuaauau auccacagcg      960

aaaguagcaa uauaugaaca acauaagaaa gaucuagcaa cacuuaaaua uuucauaaga     1020

aaauacuguc cagaaaaaua caacgcuauc uucaaagacg cgcgagagga caauuacguu     1080

gcauauuccu accacuauaa caacaaacuu gacgggcaga uuaaaaagaa agcagacacg     1140

gaaaccuuua guaaauuucu aauaaaaaua auccacucaa uaaaaccaga uaaagaagac     1200

gaaauuaucu accaagacau ggacaaucgg cucggcuuaa gacaguuuuu acccaaacaa     1260

cguaacacag auaacagggu aauaccccau caauuauaug aauaugaauu gaagaaaauu     1320

cuagagaacg cagcaaaaua ccuaucguuu uuaaaccaaa aagaagacgg cauaaguaau     1380

acagagaaaa uaauaucaau uuuuaaauuu aaaauacccu auuacguugg accacuaaau     1440

aaacacucug aauuugcaug gauagaccga aaaaguggaa aaauauugcc uuggaauuac     1500

gaaaauauga uagaugauga cgcaucagaa gaggcuuuua uaaagaaaau gacuaaucaa     1560

ugcacuuacc ucccagguga aagcguucuu ccuaaagacu cauuauguua ccaaaaguuc     1620

augguuuuaa augaaaucaa caauauuaaa guagacggaa ggaaaauuuc uguaggugca     1680

aaacaaggca ucuacaguga ccuauucuua aaauacaaga aaguaaaaag aucacagaua     1740

gaagaauacc uuauuuccaa cggacaauua gagaaaaaca gaagagaaac acuauccgga     1800

auagacauuc aaauuaaguc aucuuuaucu ucacauaucg cauuuagaag acuucuagaa     1860

cagaaaaucu uaaaugaagc ggauguagag cgcauaauag agcgagcaag uuaugcagag     1920

gauaaagguc gagucgccaa augguuaaga gcaaaauacc cucaccugac ggaagccgac     1980

auaaaauaca uauguaaggu aaaaauuaaa gacuucggac gacuaaguag aacauuucua     2040

acaggaaucg aaggagcaug uaaagaaaca ggagaggucg ccacuauuau uaguaugaug     2100

ugggaaagua acgauaaucu gauggaacua uuaagcgaac guuauacguu ugcugauaca     2160

auacuugagu ucaaaucgga cuacuauaca gaaagaaaac aaacccuuag cgaaagacug     2220

gaugaaaugu acguaucuaa ugccguacga cgaccgauau acagaacauu agacauagug     2280

aaagaucucg aaaaagcauu uggaaaaccu gagaagauau ucauagaaau gacacgggga     2340

gccaugcccg aacuaaaagg aaagcguaca aaaucacgac aacaacaauu acuugaauac     2400

uaugauaaau guaaagaaga agacguacga gacuuaaaac aacaauuaga agcauuagga     2460

gaauauguag auaacaaacu ucagucagac cgacuguuuc uauauuuuau gcaauuuggu     2520

aaaugcgccu acucuggcau gccaaucuca cuagaacgac uaauggcagg aucaaaagaa     2580

uacgauauag accauaucua cccgcaagca uacguaaagg augauaguau aauuaauaac     2640

aaaguauuag uacuaagcgu agcuaaugga gaaaagaaag acguauaucc aauuaaaagc     2700

gaaauacgca acaaaaugca gaaaacaugg aguuucuggc accauguagg uacuauaucc     2760

gaugaaaaau auaaaagacu cauaagaagu acaccauuca cugaagacga aaaauauggu     2820

uucauuaaca ggcaacuaac agaaacaucu caaucaacca aaguaguagc cgaauuauua     2880

aaagagagau auccagaagc cgaaauuguc uacucaaaag cugguuuaau aucugacuuu     2940

agacaugagu ucgaccuaua caaaucaaga aguuacaaug auuuacauca ugcuguugau     3000

gcauaccuaa auauuguagu cggaaacgua uaccacauga gauucucaag acaaugguuc     3060

aacgucaacu caucauacag uauaaaaaca aaaacucuuu uuacccacac aguaaacugu     3120

aacggaaagg aaguauggaa caaagauaug cuaccaggag uucuaaaaac cgcuaagaaa     3180

aacaccgcgc auuucacaaa acaugcaaca uuuaaaacag gcgguuuauu ugaccaaaau     3240

ccgguuaaga aagcaccugg auuaacaccu uuaaaagcag gacuacccac agaaaaauac     3300

ggagguuaua auaaagcugg cguaauguuc uucauaccag ugagauauaa auccgggaag     3360

aaaacaguau ugaugguacu aucaguagaa uuacuuuaug gaaauagauu uuuagaagac     3420

gagauauucg ccaaagaaua ugcaaaagac agauuacaaa ggaugauagg aaaaagcgua     3480

gaugaaauag auuucccaau uggaaugcgu ccauggaaag uaaacacaau cuuaaguuua     3540

gacggauuca gaauauguau aacuggaaac gcauccgggg gaaaaugucu aauugcacaa     3600

ccaauaaugc aauuuucauc agacgaauau uggaaauacu accuaaagaa aguugaaaaa     3660

uuuguagaaa aagucaaaaa uaauaguucg uauguauaug auguugacua cgaugccguc     3720

caacaugaag acaauuuaaa acuguaugau uuauauuuag auaaacugca aaacagcauu     3780

uaucguaaaa gaauaaaugc uccaauacaa acacuaauag aaggaagaga aaaauuuauu     3840

gacuguucag uaauagaaca augccaggua cucuuaaaca uacaccaagu auuuggucgu     3900

augacaucag gcugugaucu aacauuaauu ggugguaaaa caagaucagc aaguacagua     3960

aacuuuagua guaucaucuu aaauuggaag aaaaauuaca cagacguaag aauuauagac     4020

caaagugcua gcggcuuaug ggagguugua ucagaaaaca uacuagagua ccuuuga        4077


<210>  5378
<211>  4077
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5378
augggaaaaa uguauuaucu ugguuuagau aucgguacua acaguguagg auacgcaguu       60

acugauccaa gcuaccauuu auuaaaauuc aaaggagaac caaugugggg ugcccaugug      120

uuugcagccg guaaccaauc agcagaaaga agaaguuuca gaacaaguag acguagacua      180

gauaggagac agcaaagggu uaaauuagua caagaaauau uugcuccagu aauuagcccu      240

auagauccaa gguuuuucau aagauuacac gaaucagcac uauggagaga cgauguagcc      300

gaaacggaca aacacauuuu cuuuaaugac ccaacuuaca cagauaaaga auauuacuca      360

gacuauccua ccauucacca ccucauaguu gaucuuaugg aaucuucaga aaaacacgau      420

ccaaggcuag uauauuuagc agucgcaugg cuaguagccc auagaggaca cuucuuaaau      480

gaaguggaca aagacaauau aggugacguu cuaucuuucg acgcauucua ccccgaauuu      540

cuagcauucu uaagcgacaa uggaguauca ccguggguuu gugaaucaaa agcacuacaa      600

gcaacccuau ugucucguaa uucagugaac gauaaauaca aagcacuaaa aagccuaauu      660

uuuggaagcc aaaaaccaga agauaacuuu gacgcaaaca uaucagaaga cggccuaaua      720

caacuccuag cugggaagaa aguuaaaguc aauaaauuau ucccucaaga gucaaaugau      780

gcaucauuua cacugaauga uaaagaagau gcuaucgaag agaucuuagg uacacucacu      840

ccugaugagu gcgaauggau agcacauaua aggcgacucu uugacugggc aaucaugaaa      900

caugcacuaa aagauggccg aacuaucuca gaaaguaaag uuaaacuaua ugaacaacau      960

caccacgacu uaacccaacu aaaauacuuc guaaaaacuu accuugccaa agaauacgac     1020

gauauauucc ggaauguaga cucagaaacc acuaagaauu acguagcaua uaguuaccac     1080

guaaaggaag uaaaagguac acuuccuaaa aacaaggcaa cucaagaaga auuuuguaaa     1140

uacguacugg gaaaagucaa aaacauugaa uguucggaag ccgauaaagu cgauuuugac     1200

gaaaugauac aacgccuuac agacaacagc uucaugccaa aacaagucuc aggugaaaau     1260

agggugauuc cauaccaauu auacuauuau gaacuaaaaa ccauauuaaa uaaagcugca     1320

uccuaucucc cauuucugac acaauguggg aaagaugcaa uuagcaauca agacaaacug     1380

cuaaguauca ugaccuuuag gauucccuau uuugugggac cacuuagaaa agacaacuca     1440

gaacaugccu ggcuugagag aaaagcagga aaaauauauc cauggaauuu caacgauaaa     1500

gucgaccuug acaaaagcga ggaagcauuc auacguagaa ugaccaauac augcacauau     1560

uacccaggcg aggauguuuu accccuugac ucucuaauuu acgaaaaguu caugauauua     1620

aaugaaauaa auaacaucag gauugacggc uacccaaucu ccguagacgu uaaacaacaa     1680

guauucggac uauuugaaaa gaaacgcagg guaacgguaa aagauauuca aaauuuacua     1740

cucucauuag gagcauuaga uaaacaugga aaauuaacag gcauagauac aacuauacau     1800

ucaaacuaca auacauauca ccacuucaaa ucucugaugg aacgaggggu acuaacaaga     1860

gaugacguag aacgaauagu agaaagaaug acauauucag augacacaaa acgcgugaga     1920

cuaugguuga auaauaauua uggcacacug acagcggacg augucaaaca uauaaguaga     1980

cuuagaaaac acgauuuugg uagauuaagu aaaauguuuc uuacaggacu gaagggcguu     2040

cacaaagaaa ccggagaaag agcuucaauu uuagacuuua uguggaauac aaaugauaau     2100

cuaaugcaac uacugucaga auguuauaca uucucggaug aaauaacaaa auuacaagaa     2160

gcauauuaug cuaaagcaca acucagucua aacgacuuuc uagacuccau guauauaucc     2220

aacgcaguga agcgaccaau cuacagaacu uuagcgguug ucaacgacau acguaaagcc     2280

uguggaaccg caccaaagcg aauauuuaua gaaauggcaa gagacggaga auccaaaaag     2340

aaaagauccg ugacaagacg agaacaaauu aaaaaccuau aucgcucaau acgaaaagau     2400

uuucaacaag aaguagauuu ccuggagaag aucuuagaaa auaaaucgga cggacaacua     2460

cagucggaug cucuuuaccu cuacuucgcc caacuuggaa gagacaugua caccggggac     2520

ccaauuaaac ucgaacacau caaagaucag agcuucuaua acauagauca uauauaucca     2580

caaucaaugg ugaaagacga cucauuagac aacaaagucc uaguacaguc agaaauaaac     2640

ggugaaaaau cuaguaggua uccacucgau gccgcgauac gaaacaaaau gaagccauug     2700

ugggaugccu acuacaacca uggauuaaua ucucucaaga aauaccagag gcuuaccaga     2760

ucaacaccau ucacggacga cgaaaaaugg gauuuuauaa auagacaauu aguagagaca     2820

cgacaaagca caaaagcauu agcaauucua uuaaaaagaa aauuuccaga cacugaaaua     2880

gucuacucua aagcaggacu aucaagcgac uuccgacacg aauuuggauu aguaaaaagu     2940

agaaauauca acgaccuaca ucaugcuaaa gacgcauucc uagcaauagu gacuggcaac     3000

guuuaccacg aacgauucaa cagaagaugg uuuaugguaa aucaaccaua uaguguaaaa     3060

accaaaacgc uauuuacaca cucaauuaaa aacggcaauu uuguggcaug gaacggagaa     3120

gaagauuuag ggagaauagu gaaaauguua aaacaaaaca aaaacacaau acacuucaca     3180

cgguucucuu uugaccgaaa agaaggccuc uucgauauac aaccacucaa agcguccaca     3240

gguuuaguac cccgaaaagc uggacuagau guagucaaau acggcggcua cgauaaauca     3300

acagcagcau acuaucuucu aguacgauuc acgcucgaag acaagaaaac ucaacacaaa     3360

cugaugauga uacccguaga gggguuauau aaagcaagga uagaccacga caaagaauuu     3420

cuuaccgacu augcgcaaac aacuauuucu gagauccuac agaaagauaa acaaaaaguc     3480

auuaacauaa uguuuccuau ggguacaaga cacauuaaau uaaauaguau gauuucaauu     3540

gauggcuucu auuuaaguau aggcggaaaa ucaucaaaag guaaaucggu ucuaugucau     3600

gcgaugguac cccuaauagu uccgcacaaa auagaaugcu acauaaaagc aauggaaucc     3660

uuugcaagaa aauuuaaaga aaacaacaaa cuaagaauag uugaaaaauu cgacaaaaua     3720

acaguagaag acaacuuaaa ccuuuacgaa uuauuuuugc aaaaacucca acauaaucca     3780

uacaacaaau uuuucucaac ucaauucgau gucuugacca acggccgauc uacauucaca     3840

aaacuuucac cagaagaaca aguacaaaca uuacuaaaca uuuuauccau uuuuaaaaca     3900

ugcagaaguu caggaugcga ucuaaaauca auaaacgggu cggcucaagc agcuaggauu     3960

augauaagug cagacuuaac aggacuuucg aagaaauacu cagacauacg acuuguggaa     4020

caaagugcau caggacuauu cgugucuaaa ucacaaaauc uuuuagaaua ccucuga        4077


<210>  5379
<211>  4083
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5379
augacaaaac uagguaaacc auacggcauu gggguagaca uugguagcaa cucuauuggu       60

uucgcugcug uugacgaaaa cucacaucua auaagacuaa aagguaaaac gguaauaggc      120

gcucgacuau ucgaagaagg aaaggcagca gcagaccgac gagcuucacg cacuacucga      180

cgacgcuuau caagaaaccg auggaggcuc ucuuuccuuc gagauuucuu cgaaucacac      240

auaaccccua cugacccaaa uuucuucaug agacaaaaau auuccgaaau cucaccaaaa      300

gauaaagcua gauacaaaua cgaaaagcga uuauucaacg acaggacuga cgcagaauuu      360

uaucaacaau auccuacuau guaccauuua cgaacccgac uguugaccga cccaucuaaa      420

gcagaugucc gagaaauaua uuuugcuaua caucacaucu uaaagagucg aggacauuuc      480

cucaccucag gguaugcaaa ggacuucaau acaaacaaag uugcccucaa ugagauauuu      540

ccagcccuac aagacgcaua cgcacaaguu uauccagacc uagacauaac cuucgaugaa      600

aacaagguua augaauuuaa aacagucuua uuagacgaaa aagcaacccc auccgacacc      660

caacgagcuu uaguaaaucu acuauuagcc gaagacggug acaaagacau uuuaaaacaa      720

caaaaacaag uacucacaga auuugcaaaa gcuguaguag gacuaaagac aaaguuaaau      780

guugcuuugg ggacugaagu agauucaucu gaagccacag cauggaauuu uucguugggg      840

caauuggacg acaaaugggc uggaauagaa agugcaauga cugaugaagg uacagaaauu      900

uuagaucaaa uaagagaucu auacagagcc agacuauuaa auggcauagu gccagcaggc      960

aaaacucuau cacaagcaaa agucgaugau uacgaacaac accaugaaga ccuagaacuu     1020

uucaaagacu aucuaaagca acuugaagau gacggaaugg caaaagcgau aagacaauua     1080

uacgacagau acauagaugg agacgaugcc aaaccauuug ucagagaaga uuuuguuaaa     1140

gcauuaacaa aagaagugac cgcccaccca aacgcuaaau caccagaacu auuagaacga     1200

cuuguucaac cagacuucau gcuaaaacaa aggaauaagg caaauggggc aauaccuguc     1260

caaaugcaac aacgagaauu agaccaaaua auuaaaaacc agucuguuua uuacgauugg     1320

cuagcagcuc caaacccugu cgaaaaacau cgucaaucaa ugccauacca auuugaugaa     1380

cuauuaaauu uuagaauacc uuacuacgua ggaccacugg uaacagcaaa agagcaaaaa     1440

gccgcacaag ggggaguauu cgcguggaug guaagaaagg acccagaugg gaauauaaca     1500

ccuuauaacu ucgacgaaaa aguagaucgu gaagcaucag caaacaccuu uauacaaaga     1560

augaaaacca cagacaccua ccuaauugga gaagauguau uaccgaaaca aagccuccuc     1620

uaucaguccu acgaaguauu aaaugaauua aacaacgugc gaguuaacga uaacaaauua     1680

ucauucgacc agaaacaaca agucuuagga gaacuauucg aaaggcauaa uacaguaaca     1740

auuaaacaau ucgucgacaa cuuacuagcu cauggagauu acgaccgaau cccugaagua     1800

agaggacuug cugaugagaa aagauuucua aguagcuuau ccacauauaa ccaguugaaa     1860

ggcauucuac augaagcaau agacgacuca gauaagcaaa cggacauaga gaaaauaauu     1920

gcauggagca ccauauucga agacaaagcc aucuucaaaa cuaaacugaa ugaaauaagu     1980

uggcuuaaug cugguaugau aaauucacuc gcaaacauua gguacagagg auggggccaa     2040

uucucacaca aauuauuaaa cgaacuaacc cuauccacag guaggaccgu auucgaagaa     2100

cuacuaauua guucacauaa uuuaaugcaa auacuaacag acgaaguacu aaaacagaaa     2160

auagcagaau uaaaugccca cgaacuaaaa gcaagcggaa uauaugcagc aauagaugac     2220

gcuuauaccu cacccucaaa uaagaaagca cuacggcaag uccuaucagu aauagaugac     2280

auaaccaaug cugcuggaca agcaccaaac uggcuauaua uagaaacagc agauggugac     2340

ggaguaccag gcaaaagaac aaacucucgc cagagaaaga uucaggaaau auaugugaau     2400

gcugcacaag aacucauaac uggaagcgua cggggugaac uugaagacaa gauagcaagc     2460

agagcagauu ucacagaccg auuagucuua uauuucaugc aagggggaag agauauauac     2520

acaggcgaac cacuuaauau agacaaucua uccucuuaug acauagacca uauucuacca     2580

caauccuuaa uuaaagauaa cucacuugac aauaggguau uaguacaucc cuuaguaaau     2640

cgagaaaaag acacaaucuu cgcauccacu cgcuuugcaa aacaaaugaa uggaaugugg     2700

cgacaguggc acgcagcagg uuuaauuagc agucgaaaac uacgaaacuu acaaaugaga     2760

cccgacgaaa uugacaaaua ugccucuggu uucguagcca gacagcuagu agaaacuaga     2820

caaauuauaa aauuaacaga acaaaucguu gcugaccaau acccagacgc aaaaauuaua     2880

gcuguuaaag caggacuauc aucucaacua cggaaagagc uggacuuccc caaaaacagg     2940

gaagucaacc acuaccauca ugcauuugau gcuuuccuag cagccagaau cggaacauau     3000

cugcuaaaac gcuacccaaa ccucgaacca uucuuuacau acggaaaauu uaagaaaaca     3060

gaaguaaaga aauuaaaaag uuucaauuuc auaagagaca ugacacaugc aaaagauaaa     3120

auugucgcca aagaaaccgg agaaaucgua ugggauaacg caucagauau aaaugaacuc     3180

gauagaauau auaauuucaa acguaugcug auuacgcaug aaguaagauu ugaaacagcg     3240

ucauuauuca aacaaacauu guaugcagcc aaaaacagua aaaauagagg aggcucaaga     3300

caauuaauac cgaaaaagaa aggauauuua guagauaucu auggagguua uacucaagaa     3360

acaggaucau acuuaucugu agucaggcua acuaagaaag caauguacgc cguagugaaa     3420

guuagcaccc gagaugccgc gaaauuagca guagccaaau cuauaucgga acaaaaagaa     3480

aaugaaacac uaaagaaaau aauugacggu aaauuaucua aaacauccaa aaaggguaaa     3540

accacucauc aauuauuuga aauuguauua ccacgcgucg gacagaaaac uuuauuuaaa     3600

aauucuaaau acaaucaauu ccuaguaaac agugauaccu acaugcacaa uuaucaagaa     3660

uuauggaugc caagagaaua ucaaaggaug uggaaagaca uacuucuaag uaaccacgga     3720

gaugcucaau uagaaggcca auuagaucaa auauucaaau ucauagucag ucaaguaaau     3780

aguuacuuua auuuauauga caucaaucaa uuccgaaaga aaauugcaga ugcuacugac     3840

aaauuugcca aauugccuac ccacgauaca gauaaugccc gagguaaaau agccacaauc     3900

ggacaacuuu uaauaggacu ccaagccaau gcaaugaggu cagaucuaag aaacuuggau     3960

auaaaaacuc cuuucggccu cuugcaaauc gguacaggaa uaacccuuga uuuagauaca     4020

uccauaauau aucaaucucc aacaggacua uuugaacgag aaguacccuu aacugacuug     4080

uga                                                                   4083


<210>  5380
<211>  4083
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5380
augacaaaac uuaaucaacc guauggaaua gggcuagaca uaggaucaag cagcauaggc       60

uuugcuguag uugaugcuaa cagccaucua uuaagacuaa aaggagaaac agcaauagga      120

gccagauuau ucagggaagg agaaucagcc gcagguagac gcgccaguag aacgaccaga      180

cggagacucu cacacaauag auggagauua agauuccuuc aagacuuuuu cgauccacau      240

aucucuagua uagacccaga cuucuuucuc cgacaaaaau acagcgaaau aucaccaaaa      300

gauaaagaac gauuuaaaua ugagaaaagg uuauuuaacg acagaaccga ugcacaauuu      360

uauaaacaau auccuacaau guaucauuug agacuacacc uuaugaauga ugcaaguaaa      420

gcugacccac gagaaaucuu cuuugccaua caucauauac uaaaaucacg uggacauuuu      480

cuaaccccag gugacgcuaa aaauuuuaau acagacgacg uagacuugaa gcauauauuc      540

ccagcacuaa cagaagccua cgcacaagca uauccagacc uaacauugag uuuugaacca      600

aguaaagccg agaaauucaa aaguauuuua cucgaugaag augccaccag caacgauacu      660

cagaaagccc uagugaaacu ucuacuaucg gacgauggag acaaagaagu cuuaaaacaa      720

caaaagaagg uucuaacaga guucgcaaaa gccguagcug gacuuaaagu aaaauuuaau      780

guagcacuag guauagaagu uacugaugcu gauguugaaa aauggcacuu ugggauggca      840

caacuagaag auaaauggag cgacaucgaa cccagccuua cugaucaagg uauggaaauu      900

uuugaacaaa uacaggacuu auacagagcu agacuacuaa auggcauagu cccagccggu      960

aaaacauuau cacaagcaaa aguagacgau uacgaucaac acugcaagga ccuaaaacuu     1020

uucaaagaau accuaaaaca auuaaaugau caugaucuag caaaacgcau acgauuacuu     1080

uacgaucgau auauagaugg cgaugaugcu aagccguuuu uacgcgauga cuuuguuaaa     1140

gcauuaaaga aagaagugac agcgcauccc accgaacucg ccgaaucacu auuagagaga     1200

auggaacaag acaacuuuau gcuuaaacaa agaacuaagg caaauggguc aauaccugua     1260

caaaugcaac agcgagaacu agauaaaaua auugcaaauc aagcagaaua cuacucuugg     1320

cuagcagcag aaaacccugu caaagaacau agagacucga aaccguauca auuagacgaa     1380

cuauuaaacu uuagaauacc auauuaugua gguccaauga uaaccaagaa acaacaagaa     1440

aaaucaggcc aaaacguguu cgcauggaug guacgaaaag auccuagugg aaauauaaca     1500

ccuuauaacu ucgacgaaaa aguugacagg gaagccagcg ccaaugccuu uauacagaga     1560

augaaaacaa cagacacaua uuuaauugga gaagacguac ucccaaaaca gagucuacua     1620

uaucagaaau acgaaguacu uaaugaauua aauaacguac gaguaaacgg cgaauccuua     1680

agcacagacc aaaagaaaag auuaauaaga gaacuguucg aacgacacuc auccguuaca     1740

uuaaaacaau uuguagaaaa cuuaguggca cauggagaug uggcaaaacg accugacgua     1800

agaggacucg ccgacgagaa acgauuuuug agcucgcuuu caacauauca ucaacuacga     1860

ggaauccuac acgacgcaau agaugauagg ggaaaacaau ccgacauaga aaauauaaua     1920

acauggagca caguauuuga agaugcaagc auauuuaaau cuaaacuagg agaaauuuca     1980

uggcuggauu caaaaacacg aacaacguua ucuaauauac gauaucgugg auggggacaa     2040

uucucuagaa aauuacuuga cggccuaaaa cuagggaacg gacacacaau uauacaggag     2100

uuaaugcucu caucaagaaa uuuaaugcaa auauuaaccg augaaacacu caagaaagag     2160

auggcuacac uaaaucaaga acgauuaaaa cccgacagca uaaaugaagc cauugaugcu     2220

gcauacacau cucccucaaa uaagaaagcg auacgucaag uuuuaaaagu agucgaugau     2280

auuaagcaug cagcgaacga ccaggcaccc aguugguuau acauagaaac ggccgaugga     2340

gggggacauc caggaaagcg aacacuaucu cgccaaaaac aaauacaagc aauauaugag     2400

aacacagcua aagaauuaau ugauuccgcu guaagagggg aauuaaaaga caaaauagca     2460

gacaaaacaa auuucaguga cagguuggua cuauacuuua ugcaaggugg acaugacauu     2520

uacacuggaa cagcuuuaaa caucgaccaa uuaucuaguu acgauauuga ccauauauua     2580

ccucaaucac uaauaaaaga cgacucucua gauaaccgag uauuaguuca ugcaaaaaua     2640

aaccgagaaa agaacgccga guucgcauca acccuauucg cagcaaaaau ggcaucaacg     2700

uggcauaggu ggcgugacgc cgggcuaauu ucagggagaa aacuuaaaaa uuuauuaaug     2760

aggccagaug aagucgacaa auaugcgaaa ggauuuguug cucgacaguu agucgaaaca     2820

agacaaauua uaaaauuaac cgaacaaauu guagccgcac aauaccccga cacaaaaaua     2880

auagcuguaa aagcuggauu gagccaccaa cuacgacaag aacuugacuu ucccaaaaau     2940

agagauguca accacuauca ucacgccuuu gaugcauuuu uggcagcaag aauaggaaca     3000

uaccucuuaa aacgcuaucc aaacuuggcg ccauucuuua cguauggaga auuuacgaag     3060

acuaauuuaa agaaacuaca aucguuuaau uuuauaggcg cguuaacaca cacagacggu     3120

aaaaucacuu cuaaagacac aggggaaguc cuaugggaca accaaagaga uauagaggaa     3180

cuagaccaaa uuuacaauuu uaaacguaug cuaauuacac acgaaguuua cuucgaaaca     3240

gcagggcuau uuaaacagac aguauacgca gcaaaagacu cuaaagagcg uggcgguucg     3300

cgucaacuaa uaccaaagaa gaaaggauac agcacaggaa uuuauggcgg guauacacag     3360

gaaacagggu ccuauaaugc ucuaguaagg cuaacaaaag cagguaaagu uguuuacaga     3420

gucuucaaaa uuagcacaca aaacgcagcu gaaauugcag ccgcaagaag uuuauccgcc     3480

gaagaaggaa augaaagauu augccaaaua cuugaacaau cuuuacuaaa aacgaauaaa     3540

gacaagaaaa acaccguuga ccuuuucgau guaguuauuc cacgauuagg uaaagaagca     3600

cuauuucaua auaguaaaua uggcuuguuu cucgugaaua gcgacacgua uuaucauaau     3660

uaccaagaac uauggcuauc acgcgagaac cagaaaaucc uaaagaaacu auuuucaaca     3720

aaauacgauc aaagugaaau ggacagagac gcauuacauu uauucgaaac aauagcuaaa     3780

caaguagaac aauucuuuaa acuauacgau auaaaucaau ucagaacaaa aauuagcucu     3840

gccuuugaug ccuuugaaaa auuacccguu gaaggagaag aagggaaagu ggagacacua     3900

aaacaacuac ucauuggacu acaugcaaau ccuguacgau cagaucuuaa aaaucuaggg     3960

auaaaaacac ccuuuggguu uuuacaagca ggaaaaggca uacaccucga ugaugauacu     4020

gaaaucauac aacaaucacc agcaggacua uucaaacgac gaguaaggcu cgcggauuua     4080

uga                                                                   4083


<210>  5381
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5381
augacaaaau uaggaaaacc auauggaaua ggaguagaua uaggaucaaa cucuaucgga       60

uuugcagcug uagaugaaaa uagucaccua auaagacuaa aagggaaaac aguaauagga      120

gccagguuau uugaggaagg uaaagcagcg gccgaccgcc gagcuagucg caccacuagg      180

agacgacuau cuagaaacag auggcggcua ucauuccuuc gggacuucuu ugaaucccau      240

auaacaccua caggaccuaa cuuuuucaug cgccaaaaau acucagaaau auccccuaaa      300

gauaaagcaa gauauaaaua ugagaaaaga uuauuuaacg auaggaccga cgcugaauuu      360

uaccaacaau aucccacuau guaccacuua agaaaucgac uucuaaccga uccaucuaaa      420

gcagauguac gggaaauaua uuucgccauc caccacauac uuaaaucaag aggacacuuu      480

uuaacgagcg gcuaugcaaa agauuucaau accaauaaag uugcacuuaa ugaaauuuuu      540

ccagcgcuuc aggacgcaua ugcacaagua uauccagauu uggauauaac auucgaugaa      600

aauaaaguca acgaguucaa aacaguacua uugaaugaaa aagcuacacc aucugacaca      660

caaagagcac ucgugaauuu acuccuagcc gaagauggug auaaagauau cuuaaaacaa      720

caaaaacaag uacuuacuga auuugcaaag gcuguaguag gucucaagac aaaauuaaac      780

guugcucuug gaaccgaagu agauagcucc gaagcaacag uuuggaauuu uucacuugga      840

caacuagaug auaaaugggc cggcauugaa ucagccauga cagaugaagg caccgaaaua      900

cuagaccaaa uaagagaccu auaccgagcu agacuacuaa acgggauagu cccugcagga      960

aaaacccuau cacaagcgaa aguugaugau uacgcacaac aucacgaaga ccuugaacug     1020

uuuaaagacu aucuaaaaca auuagaagac gacgguacug ccaaagcaau aaggcaguua     1080

uacgacagau auauagacgg agacgacgca aaaccuuuug uccgagaaga cuucguaaag     1140

gcauuaacua aagaaguuac agcucacccc aaugcaaaaa gcccagaacu cuuagagagg     1200

cuagcacaac cagacuuuau gcugaaacaa cgcaauaaag caaauggggc aauucccgua     1260

caaaugcaac aaagagaauu agaccaaauu auuaaaaauc aaaguguaua cuacgauugg     1320

cuagcugccc caaauccagu agagaaacau cgaaaaagua ugccauauca auuagacgaa     1380

cuauuaaacu ucagaauacc auacuacgua ggcccccuag uaacagcaaa agaacaaaag     1440

gccgcacagg guggcguguu cgcguggaug guuagaaaag auccagacgg aaacauaaca     1500

ccauauaacu ucgaugaaaa aguagaucga gaagcaagug caaauacguu cauucaaaga     1560

augaaaacua cagacacaua ccuaaucggc gaagauguac uaccaaaaca aaguuuauug     1620

uaccaaagau acgaaguacu aaacgaauua aacaauguac guguuaauaa ugaaaagcua     1680

ucaauugaac aaaagcaaca agugauaaga gaacuuuuug aaagacauaa uaguguuacu     1740

auuaaacaau ucgccgaaaa ccuacgcgca cauggagacu augcccauau uccagaaaua     1800

agaggccuag cggacgaaaa acgguuccuc ucuagcuuau caacauaccg ccaauuaaaa     1860

ucauuguuac ccgaugccau agaugaucca gcuaaacaag cagauaucga aaauauaaua     1920

gcauggagca caguuuuuga agaugcagca auuuuuaaaa caaaacuuaa agaaauaaau     1980

ugguuagaua gccaaacaau aacuaaacuu aguaauauaa gguaccgagg uuggggucag     2040

uuuucucaua aauuucucaa uggacuuaca cuagguaaug ggcacacuau cauucaagag     2100

uuguuucuua guaauaauaa uuuaaugcaa auauuaaccg augaaacucu ccaaaagaaa     2160

augacagaac ugaacgcaga uaaacuuaaa gcugccaaca uuaaugacgc gauagaugau     2220

gcauauacga gccccucaaa uaaaaaggcc uuaagacaag ucuuaagggu aauagaagau     2280

auuaaaagag cagccgacgg ccaagaucca aguugguuau acguugaaac agcagauggu     2340

ggagggaccc cagguaaaag aacacgagca cgucaacacc aauuacaaga aaucuacgca     2400

aaugcagcuc acgaacucau agacagcgca guuagaggug agcucgaaga uaaaauagcu     2460

gauaaagcag acuucaacga uaggcuugua cucuacuuca ugcaaggugg ccgugauaua     2520

uacacaggua caccacuaaa uauagaccaa cuauccucau acgauauaga ccauauucua     2580

ccacaaaguc uaauaaaaga uaauucauua gauaauagag ugcucguaca cgcuauaaua     2640

aauagagaga aaaaugccac uuuugcauca acgauauacg cacaaaagau gaaugcaacu     2700

uggcggcaau ggcaugaagc uggucucauu aguggacgaa aauuaagaaa ccugcagaug     2760

aggccggauc aaauagacaa auacgccucu ggauuugucg cuagacaauu agucgaaacc     2820

cgacaaauaa uaaagcugac agagcaaauu guagccgcac aauauccuga aacaaaaaua     2880

auugccguaa aagccggauu aagccaccaa cucagggaag aauuagaauu uccuaaaaau     2940

cgcgauguaa aucauuacca ccaugcguuc gaugcguucu uagcagccag aauaggcacc     3000

uaccuccuca agagauuccc aaacuuacag gcauucuuua cauauggcaa auuuaagaaa     3060

gcugauguua agaaacuucg cggcuuuaau uuuauccgug auauaacaca ugcagaagau     3120

aaaaucgucg ugaaagauac cggcgagguu aucugggaua agaaaagaga cguugaugaa     3180

uuagaucgaa uuuacaacuu caaacggaug uuaauaacac augaggucca cuuugaaaaa     3240

gccgaccugu uuaaacaaac uguuuaugga gcaaaagacu caaaagaggc ugggggaaag     3300

aaacaauuaa uaagcaagaa gaaaggauau ccaguagaca uauauggagg auauacacaa     3360

gaaacuggca gcuaucuauc aguagucagg cuaacaaaga aagccaugua cgccguugua     3420

aaaguaagca caagagacgc agcuaaacua gccguagcca aaucaauuag ugaacaaaaa     3480

gaaaacgaaa cucuuaauga uauaaucgau gaaaaacuau caaaaauaag uaaaaacgga     3540

aaaauaacuc augagcuauu cgaaguagua cuaccacggg ugggucagaa aacauuauuu     3600

aaaaauucaa aauacaaucu cuuccucgua aauucagaca cguacaugca caauuaccaa     3660

gaacuaugga ugccacgaga auaccagaaa auguggaaag auauaucacu aucgaaauau     3720

ggagacgcac aaacagaaga ucaacuugau caaauauucg gcuuuauagu cagucaaguc     3780

aacucuuauu uuaaucuaua ugacauaaau caauuuagaa agaaauuaaa ugacgcagca     3840

gauaaauucg caacccuacc aaugcgagau acugaugaca ugcaagggaa aauagcaacc     3900

auagggcaac uacuaaucgg acuucaagcc aaugcagcuc gaagcgaccu ccgaaauuua     3960

gauauaaaaa caccauuagg auuauuacaa auaccaaacg gaauaucauu ggacagugac     4020

acuuccguag uuuaucaaag cccaacagga cucuuagaac gggaagucca uuuaagugac     4080

uuauga                                                                4086


<210>  5382
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5382
augaagaaac ccuacagcau uggucuggau auaggaacaa acucagucgg augguccgua       60

gugaccgacg acuacaaagu accagcgaag aaaaugaaag ucuuggguaa uacagauaaa      120

gaauacauca agaaaaacuu aauaggagca cuauuauuug auagugggga gacggcugag      180

gcaacucgaa ugaaaagaac agcacgcaga cgauacacca gaaggagaaa caggauauua      240

uauuugcaag acauauuuuc cccugaacua aaccaaguug acgaauccuu cuugcacagg      300

uuggaugauu cguuucucgu ugcagaagau aaaagaggug aaagacaugu uauuuucggu      360

aacauugcgg augagguaaa auaccauaaa gaauuuccaa cuauauacca uuuaagaaaa      420

cauuuagccg auucaagcga aaaagcggac uuaagacuug uuuaccuagc acuagcacau      480

aucauaaaau aucgaggaca cuucuuaaua gacgaaccaa ucgauauaag aaacaugaac      540

ucacagaacc uuuucaaaga guuuuuguua gcuuuugaug guauacaagu agauuguuac      600

uuagcgucaa aacacacuga cauauccgga auaaucacag caaaaauauc gaaaucucga      660

aaaguagaag caguccuaga acaauuucca gaccaaaaga aaaacuccuu cuucgguaac      720

augguuaguc uaguauucgg ucucaugcca aauuucaaau caaacuuuga auuagaugaa      780

gaugccaaac uacaauucuc aagagauuca uaugacgaag accuggaaaa ccuccuagga      840

aucauaggag augaauaugc cgacguauuu guggccgcua agaaaguaua cgacucaauu      900

cuauuaucug gaauauuaac caccaacaau cacucuacaa aagcuccacu uagcgccuca      960

augauagacc gcuacgacga acacaacuca gauaagaaac uacucagaga cuuuauuaga     1020

acaaacauag guaaagaagu cuuuaaagaa gucuuuuacg auacgagcaa aaacggauau     1080

gcaggauaua uagauggcaa aacaaaccaa gaagacuuuu acaaauauuu gaaaaaccua     1140

cuacaaaaag uugauggagg agauuacuuu aucgagaaaa uagaaagaga agauuuccua     1200

cguaaacaga gaacauuuga caauggauca auaccacacc aaguacaucu ugacgaaaug     1260

aaagcaauau uaagacgaca aggagaguuc uaucccuuuc ucaaagaaaa ugccgagaaa     1320

auacaacaaa uacuaacguu caaaauacca uacuauguag gaccgcuggc uagagguaau     1380

ucacguuucg caugggccag cuacaacagc aacgagaaaa ugacccccug gaacuucgau     1440

aacgucauug auaaaaccuc aagugcacaa gcguucauug aaagaaugac aaacaaugau     1500

uuauauuuac cugaucagaa aguacuccca aaacauucac uauuauacca aaaauucgcg     1560

guauauaacg aacuaacaaa aaucaaauau guaacagaaa caggagaagc aagauuauuc     1620

gacguguucu uaaagaaaga aauauuugac ggacuauuua agaaagaacg aaaagugacg     1680

aaaaagaaaa uuuuaaacuu ccucgauaaa aacuucgacg aauuucgcau aacagacauc     1740

cagggcuuag acaaugaaac aggaaauuuu aacgcuucau auggaacaua ucacgaccuu     1800

uugaaaauaa ucggcgacaa agaauuuaug gacuccuccg acaacgucga cguauuagaa     1860

gauauagugc uuagccuaac uuuauucgaa gaucgugaaa ugauaaaaca acgacuucua     1920

aaauaugaag auauauuuag caagaaaguu aucgcaaauc uuacucgaag acauuauacg     1980

ggcuggggac gucucagugc aaaacugauc aauggaauaa aagauaaaca uaguagaaaa     2040

acgaucuuag acuauuuaau agaugacggu cacucaaaua gaaacuucau gcaacuaauc     2100

aaugacgaua auuuaucuuu uaaagacgag auagcaaaua gucaagucau aggagaugga     2160

gaugauuuac accaaguugu acaggaacua gccggcucac cagcgauaaa gaaagguaua     2220

uuacaaucuc uuaaaauugu agacgaacuc guaaaaguaa ugggauacaa cccagaacaa     2280

auaguugucg aaauggcacg ugaaaaccaa acaaccgcuc gaggaagaaa caacucacaa     2340

caaagacuag gaagccucac aaaagcuaua caggauuuug guucagauau acuuaaaaga     2400

uacccaguag agaacaauca acuacaaaac gaccaacuau aucuauacua ucuccaaaau     2460

gguaaagaua uguauacagg ugacacgcua gacauacaua accuaagcca auacgacaua     2520

gaccauauaa uaccacaauc auucaucaaa gauaauaguc ucgauaacag aguauugacc     2580

aauucaaaau caaaccgcgg gaaaagcgac aauguaccau caaacgaagu agucaaaaga     2640

augaaaggcu ucuggcucaa acaauuagau gcgaaauuaa uaagucaaag aaaauucgac     2700

aaucuaacaa aagcagaacg aggaggacua agugcagaag acaaagcggg cuucauaaaa     2760

cgacaauuag uagaaacccg ccaaauaacu aaacacgucg cucgcauacu agacgaaagg     2820

uuuaacagag auuuugauaa aaacgacaaa agaaucagaa acguaaagau uguaacacua     2880

aaaucaaacu uaguaucaaa cuuccguaaa gaauuuggau ucuauaaagu ucgcgaaaua     2940

aacaacuuuc accacgcaca cgaugcauac cuaaaugccg ucguagcaaa agcucuguua     3000

auaagguacc ccaaauuaga gccagaguuc guguauggag aauauccuaa guacaauagc     3060

uacagagaac gaaaaucagc uacagagaaa auguucuucu acaguaacau caugaacaug     3120

uucaaaacua caauaaaacu agcagacggu agagucguug aaaaacccgu aauagaagca     3180

aacgaagaaa ccggagagau agcuugggac aaaacaaaac auuuugcaaa uguaaagaaa     3240

guucuaucau acccccaagu aaguauagug aagaaaguag aagaacaaac ugguggauuc     3300

uccaaagagu cgauauugcc uaaaggagga ucagacaaac ucaucgcuag aaagaccaaa     3360

aacaacuauc uaucaacaca aaaguacgga ggauucgauu caccuacagu agcauauagu     3420

auaauguucg uagcggacau cgaaaaaggu aaaaguaaaa gacuaaaaac aguaaaagaa     3480

augauuggaa ucacaauaau ggaacgguca agauucgaau cuaauagcgu aacauuccua     3540

gaagaaaaag gauacagaaa cauaagagag aacacuauaa uaaaauuccc caaauauuca     3600

uuguuugaac uagaaaaugg ucguagaaga cuauuagcaa gcgcaauaga acuacaaaaa     3660

gggaacgaaa uguuuuuacc ccagcaauuu guaaacuuac uauaucaugc acaacaugcu     3720

aacaaagaag acucaguaau auauuuagag aaacauagac acgaacuauc ugaacuuuuc     3780

caccacauaa uaggcguuag cgagaaaacc auuuuaaaac cuaaaguaga aaugacguua     3840

aaugaagcau ucgaaaaaca cuucgaauuu gacgaaguaa gcgaacucgc ccaaucauuc     3900

aucuccuuac ucaaauucac agcauucggu gccccuggag gauucaaauu uuuggacgcc     3960

gacauaaaac aaaguaauuu gagauaucaa acaguaacag aaguucuuuc aucaacccua     4020

auacaucaaa gcguaacggg auuauacgaa accagaauag accucucaaa gcucggaggc     4080

gaauga                                                                4086


<210>  5383
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5383
augggaaacu auuaucuagg acuagacauc ggcacuaacu cuguuggaug ggcugucacu       60

gaugagaauu acaacauaga acgguucaaa aagaaaaaua uguggggaau ccgacucuuc      120

gacgaagcua aaaccgccga aucaagacgc auacauaggc aagcucgcag aaugagacaa      180

aggaaaaucc aacgacucaa cuuacuaaug gaacuauuug acguagaaau auccaagguc      240

gacgaaagcu ucuucauacg aaaaaaagaa agcgaccucc auuaugaaga caaaagucaa      300

gacucccgau acgcauuauu caacgauaaa gaauucaaug acaaggauua ucacaacaaa      360

uauccaacua uauuccaccu aauaauggac cuuauagaaa acgacgaaaa aaaagauauc      420

agacuucucu auuuagcaug ccauuaucua auaaaaaauc ggggacauuu cauauucgaa      480

ggucaaaaau ucgacgugaa cucaucuuua aacaauaaca uaaacaaauu aaaaucacac      540

uuagaccaau ucuauaauau augucuagau uucgacaacg acaaacuaau agaaauacua      600

guuaaccaaa aucucaacau aucagaaaaa agcaaaaaaa ucaaagagcu acuacgaaaa      660

accaaccuug aaguaggaau ugcgaaccua auagcaggag gaucaaucaa auuauccaau      720

auguucgacg acgacgaacu aaaaaaccuu gauuauaacu cauuaaguuu caaaaauucu      780

aacuucgaug aaggaagaga guuauuugag gaaguacuag aagaucgcuu cgaacuugua      840

gaaaucuuaa aagacauuca ugacgcuagc guauuagaaa caaugcuauc cagugcauca      900

auaagcccag acgguggaaa auacauaucu ucaagcauga ucaacagcua caacaaacau      960

aaaagggacc ucaaauuacu aaaaaaaaua auaaaaaaau auaaaaaauc agaauaugau     1020

aauauauuuc gggacgagaa uauaaaaaac aauuacuuau cauauaccag gacaggaaua     1080

aacaaaaaua aaaaagucca agccgaagac aauacagaua gcgacgguuu uuacaaauau     1140

auagugaaga uccucaaaga uaucaaagaa aaugauucag acgaagacau caaauacauc     1200

cucucagaaa uagacaaaaa aaauuuuguu cccaaacaaa ggacaaaaga aaacuccguc     1260

auaccauauc agcuacgaga aauagaacua agaaaaauaa uugacaacca aucaaaauau     1320

cacaaauuuc ucaacgaagu agaugaaaaa ggaauaagcg ucaaagaaaa aauaauaucu     1380

cucuugaccu uccgaauacc uuacuauauu ggaccucuaa acccaagcua caaugaagaa     1440

aagaacuuca ccaccucuug gguaaaaagg cuagcaaaag guaaagucac accguggaac     1500

uuugaagaua ugguagacau cgacgcuagc agagaggcau ucauagaaaa caaacuuaac     1560

aaaugcucau uucuuuuaga cgaaacaguu cucccaaagg acucauuauu auaucaagaa     1620

uacauggucc ucaacgaacu caacaaccua aaaauaaacg gacagucaau aaacaccgac     1680

uuaaaaaaau ccauauacga agaacuauuc aaaaaauaca aaaaagugac acgcaaacga     1740

uuaauaaguu acauaaaaga aaaagaaaau augagcggcg auauaguaau aacaggaaua     1800

gauggggacu uuaaacaggg acugaaaagc uauauugccu ucaaagacau cauuggugaa     1860

aaaauagacg acgacauuua ucgcaaagaa guagagaaaa uuauuaggga uauaucauua     1920

uauggagaug acaaaucuuu uauucaaaag aaaauuaaaa augaaaacaa gcuagacuua     1980

uccgaugauc aaaucuccaa aauagccaaa uuaaaauuua aagauugggg acgcuuuucu     2040

aaaaaacuuc uaguagggau aaaggcauca gacaaaaaca cuggcgaaau agacaauauu     2100

auaggauuua ugcguaauua uaacuacaac auuaugcaac uaauggcaaa cgucuucacc     2160

uuuaaagaug aaaucgaaaa auuaaacagu aaauauuaca cagacgaaaa aaucaacauc     2220

gacuaucuag acgacuuaua ucucucauca ccagcaaaaa gaauguuaug gcaaagcuuu     2280

caaauuguag augaaauuaa aaacaucaaa aacucggacc caaaaaagau cuuuauagaa     2340

auggccagaa gcaaagaaga uaagccaaca agaaaaaugu cucguaaaag ccgcuuagag     2400

aaacucuaua ccgacaguaa gaaacaauuu auuaagcuau ucggaccaga ugaauaugac     2460

aaacuaaaca augaaauuaa aaauaaaagc gaaagugacu uuagauggga caaucuuuac     2520

cucuacuaua cacaauuugg cagaugcaug uauagccuag auccaauuga uuuagaagaa     2580

cuguccaaca aaaauuuaua cgaccaagac cacauauauc caaaaucaaa gauauacgac     2640

gauuccauag aaaauagagu uuuaguaaaa aaacaacuca aucuagauaa aagcaaugaa     2700

uacccaauac cagaucgagu auugaacaaa gauaagaaua aacuauuagc auuuuggaaa     2760

auguuaaacg aaaaggaauu aauaggagac aaaaaauaug caagacuaau aagaaaaagc     2820

cccuucacag acgaagaacu gggggauuuc aucgccagac aacugguaga aacacgacaa     2880

gcaacaaaag agaccgcaaa uauguuaaaa cgcuuaugcc ccgacaguaa aauaguauau     2940

guuaaagcaa agaauguaag ugacuucaga agaaaauuug acaucuuaaa augcagauca     3000

aucaaugacc uacaucaugc acaugacgcg uacuuaaaca uagugguagg gaacguauuc     3060

gacacaaaau ucacaagcaa cccucuaaac uuuauaaaga acaauucgaa aaacaggagu     3120

uauaaucuag aaaaaauuua cgauuaugau guaauacgaa augaaaaaau cgcaugguua     3180

gcugaaagaa aagaagaaaa ccaagacggc ucaaucgcaa ccgugcucaa aaacuuaucc     3240

aagaacgaua uaagaguaac acgacgaacg uacaucaaau gugaggaucu auucgaugaa     3300

aauaucgugc gaaaaggcaa uggaaaauuc ccaauaaaag aaaauaauaa uaaaucagau     3360

accaauaaau acgguggaua cucaggaauc aaaaaugcau acuuugucuu acuagaagac     3420

gaaaaacaua acagaauucu agaagcuauc ccaucauuca uauacaaaaa agagaaaaac     3480

ggcgaaaaag gcucagucau uaacuacuug caaaacauau uaggaauaaa auccgcaaaa     3540

auccuaagag acaaaauaaa aaucggaagc uugcucaaga ucgacaaauu ucccuacaac     3600

cuagcaggaa aauccggaaa cggcauccuu auaucaggcg gaguacaacu uauacucgac     3660

aagaacacua gcaaauacau aaaaaaaauc gaaaauuaca aauacaaacu cgaugaaaac     3720

aaggauuacc agaucaauau auuugauaag cuaagcaaag aggaaaacau uaaauuauuu     3780

gaccuauucg uagauaaauu ugaaaacagc auauacaaaa aucgcguaaa cauuaaguua     3840

aaggagauaa aacucgcacg ccccaaauuu gaacacuuau cuauagaaga acaaauaaaa     3900

acauuaacac aaauguuaau auuauucgga agagaaaaua augcuguaaa ucuugaauac     3960

auuggauugc caaaaaacac aggaaaaaca aagauaaaca aaaaaauaaa uaacaaggac     4020

aucaaaauaa ucaaucaauc aauaaccgga cuauacgaaa ccgaagaaga ccuagcuaac     4080

aucuga                                                                4086


<210>  5384
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5384
augacuaaau uaaaucaacc uuauggcauu gguuuagaca uagggagcaa cagcauugga       60

uuugcaguug uugaugcaaa cagucaucua uuacgacuaa aaggagagac ugcaaucgga      120

gccagacucu uccgagaggg acaauccgca gcagaacgaa gagaaucacg cacgacaaga      180

cgaagauuaa gcagaacacg auggcgucuc ucuuucuugc gcgacuuuuu cgcaccacac      240

auaacaaaaa uagauccaga uuucuuucuu agacaaaaau acagugaaau caguccaaaa      300

gauaaagaca gauuuaagua cgaaaagaga uuguuuaaug auaggacaga ugcugaguuc      360

uaugaagacu acccaagcau guaccauuua aggcuacauc uaaugacaca uacucauaaa      420

gcagauccac gagaaauauu cuuagccauc caccacauac uaaaaaguag aggccacuuc      480

cuaacacccg gagcagcaaa agacuucaac accgacaagg uagaucuaga agauauauuc      540

ccagcauuaa cagaagccua ugcacaaguu uaucccgauc ucgaauuaac guuugaccua      600

gcaaaggcug augauuucaa agcgaaauua cuagaugaac aagcaacacc gagugauacg      660

caaaaagcgc uaguaaaccu cuuacuauca agugauggag aaaaagaaau aguaaagaaa      720

agaaaacaag uacuuacaga guucgcaaaa gcuauuacug gauuaaaaac uaaauucaau      780

cucgcauuag ggacugaagu agacgaagcu gaugcuucua acuggcaauu uagcauggga      840

caacuggacg auaaauggag uaacauugaa accagcauga cagaccaagg uacggaaaua      900

uuugaacaaa uccaagaguu auauagagcc cgacugcuca auggaauagu accugcaggg      960

augucuuuaa gccaggcgaa aguagcugac uacggacaac acaaagagga uuuagaauug     1020

uucaaaacuu auuuaaagaa auuaaaugau cacgaauugg caaaaacaau aagaggacua     1080

uaugauagau auauuaacgg agaugacgca aaaccauuuc ugagagaaga cuuuguuaaa     1140

gcucuaacaa aagaaguaac ugcacaccca aacgaaguuu cagagcaacu auugaaccga     1200

augggacaag caaacuuuau gcuuaaacaa cguacaaaag caaacggugc uaucccugug     1260

caacuccaac aaagagaacu agaccaaauu auugcgaacc aauccaaaua uuacgacugg     1320

cucgcaaaac caaaccccgu cgaaaaacau agagcaucga aaccauacca auuagacgaa     1380

cuucuaaccu uccgaauccc auacuacguu ggcccacuca uaacuccgaa agaacaagca     1440

gaaagccgcg aaaacguguu ugcguggaug auaagaaaag acccaucagg uaauauuaca     1500

ccguauaauu uugacgaaaa ggucgauugu ccugcuagcg cuaacauguu cauacaaaga     1560

augaaaaaua cagauacaua uuugauuggc gaagacguac ugccaaaaag auccuuacuc     1620

uaccaaaaau augaaguucu aaaugaacug aacaacgucu caauagacgg uaggagauua     1680

ggagcagauc aaaaacaaag auuaauaaga gaaguauucg aacgccacuc cagugucaca     1740

auaaagcagg uagcggaaaa ucuaguagcg cauggagacu ucgcaaaaag accagagaua     1800

cgaggauuag ccgacgagaa aagauuucua ucaucccuua guacuuacca ucaacuaaag     1860

aaaauacuac augaagcaau cgacgauccu acaaaagaac acgacaucga aaacaucaua     1920

acauggucga caguguuuga agacgcagcc auauuuaaaa ccgaauuagc aaaaauaucc     1980

uggcucgacu cugacacuau caaagcauua agcaacaucc gauacagagg cuggggacaa     2040

uucucaagaa aauugcuaga cggccuaaaa cuaggcaaug gacauaccgu aauccaagaa     2100

cuaaugcuua guaaucauaa ccuaaugcaa auauugaccg acgaaacacu caagaccaca     2160

auggcagaau ugaaucagga caaauuaaaa gcagaugaca uagaagacgu cauuaaugau     2220

gcauauaccu cacccucgaa uaagaaagca uuacgacagg uauuaagagu uguagaagac     2280

auaaaacacg cggcaaacgg ccaagaucca agcugguuau auauagaaac ugcugacgga     2340

ccaggaacgg ccggaaaaag aacccaaucg agacaaaaac aaauucaaac cguauaugca     2400

aacgcagcac aagaauuaau ugacuccgca guuagaggug aacuggaaga uaagaucgca     2460

gacaaagcau cuuucacaga ucgucuagua cuauacuuca ugcaaggagg aagagacaua     2520

uauaccggug caccacuaaa cauagaucag cuaagucauu acgacaucga ucacauucuu     2580

ccacaaucau uaauuaaaga cgauucacuc gacaauagag uucuaguaaa ugcaacgaua     2640

aacagagaaa agaacaacgu guucgccucc acacuauuug caagcaaaau ggcggcaacc     2700

uggcgaaaau ggcacgaagc agggcuuaua agcggacgaa aacuaaggaa ccuuauguua     2760

agaccagacg aaauagauaa auuugcacau ggauuuguug caagacaauu aguugaaaca     2820

cgacagauca uaaaacuaac ugaacaaaua gcagcagccc aauauccaaa uacaaaaaua     2880

auagcaguga aagcaggucu aagucaucag uuacgagagg aacuagacuu uccuaaaaau     2940

agagauguua aucacuauca ucaugccuuu gacgcauuuc uugccgcucg caucgguacc     3000

uauuuauuaa aaagauaccc aaaacuugca ccuuucuuua cauaugguga guuugcgaag     3060

guagacguga agaaauuccg agaguucaau uucauaggag cacuaaccca cgccaagaaa     3120

aauauuguug cgaaagauac aggugaaaua gucugggaua aagaacggga uauacaggaa     3180

uuagaucgaa ucuauaacuu uaaaagaaug uugauuacuc augaaguaua uuuugaaaca     3240

gcagaccuuu ucaaacaaac aauauaugca gccaaagaua gcaaagaacg ggguggaagu     3300

aaacaacuaa uaccuaagaa acaaggauau cccacgcaag uauauggggg auacacaaga     3360

gaaaacacag cauaucuagc aguuguaaaa accgucgaaa agaaagccga cguguaucga     3420

gucguucgaa ucgccacauc acaggucgcu gcauuaaaag aagccagagc ccaaucaaca     3480

guaaaagaac gagaauugcu aaagaaauuc uuaacgacaa aauuuacaaa aaucggcaaa     3540

aauggaaaga aagccauaac cccauucgau aucguaauuc cgcgugugcc uagagaacaa     3600

uuauuucaua auaaaaggua uggguucuuu augguaaaua gcgacaccuu aaugcauaac     3660

uaccaggaau uauggaugag ccgaucggac caacaaaucu ugucgaaacu cguaaaaucu     3720

gcaaaaacug uugaaaaugg ucaaguaggc agacuauuug aaaacauagu ugaacaaauu     3780

aacaaauacu uuccauuaua ugauauaaau caauuuagaa agaaauuaga ccaaagcaaa     3840

gacauauuuu ccaaauuacc ccuagaaggc aauggaacaa ccucugguaa aagggaaacu     3900

auucaaaaca uauuaaccgg agcaggaaca ggagccaaaa auggugaccu caaaaaccuc     3960

gguauaaaaa caccauuugg auuucuacaa guccccagug guauuguguu agaugacgac     4020

acaacacuaa uauaccaaag cccaacuggc auguuaaacc gaauagugag acucucagaa     4080

uuauga                                                                4086


<210>  5385
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5385
augaaaaaug aagaaaacau auaucuugga cuagacauag gaacagauuc aguaggauac       60

gcaguaacag augacaaaua uaaaauaaug aaauaccaug gaaacgcagc auggggaucc      120

acgauauuug acgcgggagc auugggugca gauagaagag uauuucgcag ugcaagacga      180

agacuugaca gaagacaaga acguauucaa uugcuacaag aaauauuugc aaacgagauc      240

gcaaaaguag acgaaagauu cuuuauuaga cuucuagaau cugggcuaug gagagcagac      300

gccgaagaua gguacguauu cuucaacgac aaagaauaca ccgacgugca auacaugaga      360

gacuacccaa ccauacacca ccuaauuugu gaacuaauau acaacccaaa uaaacaugau      420

guuagguuag ucuacuuagc augcgcaugg cuaguaacuc acagagggca cuucuuaagu      480

aacauaaaua cugaaaauau agccgauuua guagauauac auaacguaua uaacggauuu      540

augagauucu uugaagaaaa cgaaauaaua ugcgacugga gggaaguuaa ugucgaagaa      600

cuagguagag uucuugcagc gaacauagga auaaaugaaa aauacagaaa cuuaaguaca      660

guacucuuug cuacuagaaa accggacaag aaagcaaaag aaggauuccc cuacuccgaa      720

gacgcuauag uuagauugcu agcgggagga acaugcaaau uaaaagaugu auacauaaaa      780

gaagaauaug augaauuugg aucaauaaca uuaggaaugg acgacgagaa aaugaccgaa      840

aucauggccg acauaggaga cgacuucgau cuaauaaaag cacuacgauc ccuauaugac      900

uggggaauac uagcaaacau uuuaguaaau gaaaauaaca gcccauuaau aagucaauca      960

aaaguuaaag uauacgaaca acaccgagaa gaccuggcca accuuaaaua uuuaguacac     1020

aaauauauuc cccacaaaua caacgaaauc uuucgcgaaa uccgagguga uaacuaugua     1080

gcauacuccu aucauuacga gcuuaaaaau gacgagaaaa uggguaagaa agcagacguu     1140

gaaaaauuuu caaaauuucu acuaaaaaua cuggaaggaa uaucaguuga ugaacaagac     1200

aaagucguau augauaauau gaucgagaga cuuaccuuaa gacuauuuuu gccaaaacag     1260

aaaaacacug acaauagagu aauaccacau caauuguaug aauacgaauu aaaaucuauu     1320

cucaaaaaua ccaaaaauua cuuacccuuu uugaaagaaa aagacaguga cggaauaagu     1380

aaugaagaca aaaucauaag cauauucaaa uuuaaaaucc cauauuuugu agggccucua     1440

aauuguaauu cacaauucgc cuggauacaa cgaaaagcag acgguaaaau auuuccaugg     1500

aauuuugaag auaaaguaga cuuugaugca ucugaacaag ccuuuaucca aagaaugacc     1560

aaucaaugua ccuauuuacc cgaagaaagc guacuaccaa aacaaucauu auacuaucaa     1620

aaauucaugg uacuuaacga aauaaauaac auaaaaaucg acggaagaaa aauauccguc     1680

gccaugaaac aagaacugua caaugaaaua uucgagaaaa agaaacgcgu caggaagaaa     1740

gauguaauag acuucuuugu uagcaacgga uaccuagaag gaggaaaaga agcauugaua     1800

ucgggacuag acgaacaaau acacacaaac cuaagcagcu uccacgcuuu ccgaagauua     1860

cuacaaaacc acauauuauc aguagaagac gucgagagca uaaucgagcg agcaucauau     1920

uccgaugaca aacgaagggu uuuauuauuc cucaaaaaua aauacucuuu ccucacagac     1980

gaagauauua aauauaucug uaaaaucaaa auaaaagacu ucggacgauu aucgaagaaa     2040

uuuuuaguug acuuugaggg uacauguaaa gaaacaggag aaaucaguac aguuuugcgg     2100

cuuauguggg aaacgaauaa caaucuuaug gaauuacugu cagacaaaua cacguuugcc     2160

aauggaauaa aagaaaauag aaagaaauau uauacagaac aacagaaaac gcucgaugau     2220

cguuuagaug aaauguacau aucaaauagu gugaaaagaa caauauacag gacucucagc     2280

gucguaaaag acguaacaaa agccuucgga guccccaaga aaauauucgu agaaauggcc     2340

agaacuaaua acccagaccu gaaaggaaaa agaacucgaa ccagacaaca acaaguaaua     2400

gaacuauacg caaaauguga cgaagaagac guaagagaau uaaaacacca aauggaaagu     2460

cuaggagaau auguagauaa caaacuacaa ucugauaaau uguuucuaua uuuuauccaa     2520

uucggcaaau cagcauauuc ugguaaaaug auagaccucg aaaaacugau gagcggaucu     2580

aaaguuuacg acauagaaca cauauaucca caagcauacg uaaaagacga cagcaucaua     2640

aauaauaaag ucuuaguauu uucagaagag aacggagcaa aacaagacau cuauccuaua     2700

aaaaccgaaa uacgaaaaca aaugagcggg auauggacca cuuggcacca uguuggugca     2760

auuuccgaug agaaauacaa acgacugacc cgcucaaccc cguuuaauga agaugaaaaa     2820

uacggcuuua uaaaucgaca acuaacugaa acaagccaau caacaaaagc aauagcacaa     2880

auccuuaaug aaaaauaccc agaaacugag auaguauacg ucaaagcugg ccuaguaagu     2940

gacuuccgac augaauuugg ccuuuguaaa uguaggagcu acaaugauuu acaccacgcc     3000

aucgacgcau accuaaaugu cguaguagga aacguuuaca acaugaaauu uaaucgaaaa     3060

ugguuuucag uugauaguaa auauucaauc aaaacaaaaa caaucuuuag ccacagccua     3120

guaugcaacg gaaaaauaau augggauaag gagaaaaugc uacccauggu ucucaaaaau     3180

gcuaagaaaa auaaugccca cuucacaaag uaccaauuua ugaaaaccgg uggauuuuuc     3240

gaccaaaugc ccugcaaaaa ggcauccggc cuagucccgc gaaagaaagg auuagauaca     3300

gaaagauaug gaggauauaa uaaaucaggg auaauguucc uaacaccaau gcguuauaaa     3360

auagggaaga aaucagacau aauaauacua agcauagagu uacucuaugg uaaauuauuc     3420

cuaaaagacg aaguauuugc caagaaauau gcggaagaga gacuacagaa aauaauaggc     3480

aagaaagcag augaaguauc auuuccaaug ggaaugaaac cuuggaaaau aaacacaguc     3540

uuaucauuag auggcuucaa uguuugcaua acuggcauag guggcggagg caaaugccuc     3600

agugcacaac cuaucaugca acuuucccug ccauacgaac uagcaaaaua uuugaagaaa     3660

auagaaaaau ucguagagaa aaacaaaaau aacgcaaauu auauauauga ugaacaauau     3720

gauucagucu caguugauga aaacauaaaa cuuuaugaug ucuaccucga uaaauuagaa     3780

aacucaauau acaacaagag aauaaacagu ccagaagcaa uucucaaaaa uggaagagaa     3840

aaauuuugua aacuaaacgu auuagagcaa gcaguaaccu uaauaaauau ccaaucgauc     3900

uuuggccgaa ugacaggugg augcgaucua gaaaauauag gaggcaagaa acauucagca     3960

gccacaguaa auuuuucauc aaauguuucc aacuggaaga aauacuauaa cgauguaaga     4020

gucguauauc aaaguccuag ugggauaugg gaaagaagau cucaaaacau acuagaacuu     4080

cuuuga                                                                4086


<210>  5386
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5386
augaacgaac ccuauggugu agguuuagac auagguacua auaguguagg auggacugua       60

guugauauga acggaagagu uagaaaagua aaagguaaaa cagcgcuagg agcacgccuc      120

uuuaaagaag gugcaacugc cgaggaccgc agaggcuucc gaacgaccag acgaagacua      180

aaacgcguca aguggagauu acggcuuuug cgagaauuuu uugaccaacc aauaucaaag      240

auagauccaa auuuuuucgc uagacgaaaa uacucagaua uaucaccaag agaucccaau      300

uacaaugguc uagcaaaaac ucucuucaau gaucgaacug acaaggaauu uuacgaugau      360

uaugcuacaa ucuaucacuu aagagacaaa uuaaugacau ccaacagaaa auuugacaua      420

agggaaauau aucuggcuau acaucauaua guuaaauaua gaggaaacuu cuuacgcaca      480

ggaccagcaa gccaauaugg cuccgccuca cuacaccuag caacaaguuu ucaaaaacua      540

aacgaucugu ucgcucaaag cgaagaaacu cuuaaucuaa aauuggugac agaugaagca      600

uuauuacaac aaauacaaca aauacucgua agaacugauu uaaguagaag cgaacaacaa      660

cgacaaaucu ggccccugau ggcuguccua accggagccu cagcagcgga gaaaaaaaga      720

caaaaaaacg uuguaguaga gcuuaguaaa gccuuaguag gacuaaaagc uaagaugaau      780

gugguaacuu uaacugaagu ugacgcugca guagucaaag auuggacguu caccauggaa      840

gaaagccaag acaaauuacc ggagauugaa gaacaacuaa gugaagucgg gcaacaaaua      900

auggaugagg ucauccaauu guaugcauca guaaaucuag cucagcuaau accagcuggu      960

aaacgauuua gucaacauau gguagaaaaa uacaaacauc augaaaaaaa ccucgaacua     1020

cuuaaagcau acauacauuc acaauccgac agcaagaggg gccgggaaau cagagccacc     1080

uaugaucgau auauugaugg cguagauucu aaaccaguaa cucaagaaau guuuuauaag     1140

gauuugauga aauauguaga agccgaugcg accucuaacc accucgcagc ugagauaaaa     1200

gacgaaauug auagugaaca auucaugccc aaauugcgaa ccaaacaaaa ugguucgauc     1260

cccuaucagg uucagcaaua ugaacucgac caaauaaucu cacaccaaaa aaaauauuau     1320

cccuggcuag gagaagaaaa uccaguggca gaaagaagag gcaaauuucc guauaaacuu     1380

gaugaacuag uuggauuuag aguacccuac uacguuggac cacuaaucac aaaggaagau     1440

caacaggcaa caucaggagc uggauuugca uggaugguua gaaaagcaga uggaccaaua     1500

acacccugga acuucgauca aaaaguagau agaauugcaa gugcaacagc cuuuauacaa     1560

agaaugcaaa caacagauac auaucugauu ggcgaagacg uacuaccagc acgaucacuu     1620

auuuaucaac gauuuauggu acucaaugag uuaaauaaca ugagaguuga agacagaaaa     1680

cuugcccccc aacaaaaaca gagguuauau aaccaaguau uuaagcaaca ccaacacgua     1740

ucaguaaaga acauacaaca aaaccuaaug gaugccgggg aauauagaaa aacuccccaa     1800

auaacaggcc uugcagaucc aaagggauuu aacucuucuc ucaguacaua ccacgacuuc     1860

aagaaaauac uucuugaggc aauugcugac gaacauaaaa gagcugauau agaaaaaaua     1920

auacuaugga gcaccacauu ugaagauucu gcaauauuca aacaaaaacu agaagaaguu     1980

gcauggcuaa cagacgcaca aagaaaacag uuaucgggcu uaagauaccg cggauggggu     2040

caacuaaguc auaaacuucu aacagcauuu aaagaugaua aaggcagauc aauuauggau     2100

ggacuguggg aaacuucuga uaauuuuaug caacucagaa aacagccagc aauagaaaca     2160

caaauaaaag aagcaaauca ggcaaacuua acugacgccg auauccaaga uacuauaaau     2220

gaauuauaua cuaguccgca aaauaaaaaa gccaucagag aagucaugcu aguauuagac     2280

gacauaaaaa acgcaaugca cggccaaaca cccaguugga ucuuuguuga ggcugcuaga     2340

ggaggaggag uagcaggccg aagaacacaa ucccgaagcu cucaaauagu cgaagcauau     2400

aaaggaaccg caaaagaaau ugugucagaa aagguacaac acgaauuaaa cgaaaaaauu     2460

aaagccaaag cugauuucaa caccaggcuc guauuauauu uuuugcaaaa cggucgagau     2520

cuauacacaa acgaagcaau aaauauagau aggcuuucag aauaugauau agaucacauu     2580

uuaccacaau cuuuagugaa agaugacuca cuagacaaua gaguccucac cagugcacga     2640

aucaauagag aaaaaaauga cacauuugca ucagaaaaau uugggagaaa aaugggugca     2700

caauggagag aauuacaucg aaauggccua augacacaaa gaaaauuaaa acaucuauug     2760

augagaccug acgaaauaag caaacaugcc acagauuuca uaaaucgcca acuuguugaa     2820

acacgacaag uaauuaaacu ugucgaagaa cucauaucaa gcgaauaccc ugcugccagu     2880

auuguagcag uuaaagcuaa ccuaacgcac caauuucguc aaaccuuuaa uuuuccuaaa     2940

cuuagagaag ucaacgauua ccaucaugcc uucgacgcuu cacugacggc uuucauagga     3000

auguaccuau uaaaacaaua uccgaaacuc gaaagauucu ucguauaugg aaaauuugcu     3060

aaacaaccaa ucaaccuaac uagauuuaau aucauacgga aacuugccgu cgcagagaaa     3120

ccaaucgcca acauagagac aggcgagaua uuaugggaua agacagcaga cauuaaauac     3180

uucgaaaaac uuuacaauua uaagagacua cuagucaccc acgagguaag agaaaacuac     3240

ggagcuaugu uuaagcagac auuauucaaa gcaucauaua acaaauccaa aacuuuagua     3300

ccaaaaaaaa aucacaugga aaccuccguc uacggcgggu acucaaacca agaaacggca     3360

uacuuagcca ucguacggau acccuuuaaa aguggauuca aguuuaucgu agucggaaua     3420

ccaacaagaa ugguagcuaa aaucaaacau uaucagucac uaggggcaac ucucaaucaa     3480

gcaacccaca aaguaauaga accaaaauuc acaaaaauuu cuagaaaaac aaaacaaaca     3540

gugauaagug auuaugaggu uguccuacca aaaguuuauc uugaccaagu aguucgcgac     3600

caaguaaaag gucaaaugua ucgauucucu uuggguucgg auaaagaaua ccauaacgua     3660

caagaacuau auuuaccucu aagcauacaa caagcuuucg uuggacauua cgaugaaucg     3720

gacgaucaac gaucaaacga ccuagucaaa guuuacgacg cagcauuaaa acaacuccaa     3780

cgauauuucc cuuuacacuu auccaggaac uuugaucaag uagccucgca ggcgcaccaa     3840

ucguucgagg gacuaaaaaa uaacguacaa acaagugaua aacaauuagg uaaaaaggaa     3900

guacuaaauu cacuauuugu aggucuacac gcuaacgcaa caaggucuaa ucuaucaguc     3960

cuagguauga guaaagacuu cggccgcauu aaaaguaaug gcauuacacu aacugaccaa     4020

gcagaaauua uuuaucaauc cccuacagga uuguucgaac gcaaagucgc auuaaaagau     4080

uuauga                                                                4086


<210>  5387
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5387
augggaaauu auuaccuugg ccuugauauc ggaacaaaua gugugggcug ggccguaacu       60

gaugaaaacu acaacauaga gagauucaag aaaaaaaaua uguggggaau cagacuauuu      120

gaugaagcaa aaacugcuga aucaaggcgg auacaccgac aagcaagaag aaugagacaa      180

cgaaaaauuc aaagguuaaa ucuccucaug gaauuauuug augucgaaau cucaaaaguu      240

gacgaauccu ucuuuauccg caaaaaagaa ucagaucucc acuaugagga uaaaucucaa      300

gacucccgcu acgccuuauu caacgauaaa gaauucaacg acaaagauua ccacaauaaa      360

uauccaacaa ucuuccaccu uauaauggac cuuauagaaa augaugaaaa aaaagacauc      420

agauuacucu acuuagccug ucauuaccua auaaaaaaua gaggucauuu cauauucgag      480

ggacaaaaau ucgauguaaa caguagccuc aauaacaaua ucaacaaacu uaaaagccac      540

cucgaucaau ucuacaauau augccuugac uucgacaacg auaaacucau cgaaauacuc      600

gucaaccaga accuaaacau aagugaaaaa ucaaaaaaaa uuaaagaauu acuaagaaaa      660

acaaaccugg aaguaggcau ugcgaaucug auugccggcg gcuccauaaa acugagcaau      720

auguucgacg acgacgaacu caaaaacuuc gauuauaauu cccuaucauu uaaaaauucc      780

aacuucgacg aagguagaga acuauucgaa gaaguuuuag aagauagauu cgaauuagug      840

gaaauacuaa aagauauuca ugaugcaucu guacuagaaa caauguuguc aucugccagc      900

aucucuccug auggaggaaa auauauaucu ucaucaauga uuaauucaua caacaaacac      960

aaacgagacc uaaaacuacu uaaaaagauu auaaagaaau auaaaaaguc cgaauacgau     1020

aauauauuuc gcgacgaaaa cauaaaaaac aacuacuuau cauauacacg uacaggaaua     1080

aauaagaaua aaaaagucca agcaaaagac aauacugaua gcgacggcuu uuauaaauac     1140

auuguuaaaa uuuuaaaaga uaucaaagaa aaugacucag augaagacau caaauacaua     1200

uugucagaaa uagacaagaa aaacuucguu ccuaaacaaa gaacuaaaga aaacucugua     1260

auaccauauc aacuaaggga aauagaauua cggaaaauaa uagauaacca aagcaaauac     1320

cacaaauuuc uaaacgaagu ugaugaaaaa ggaauaagcg uaaaagaaaa aauaauagca     1380

uuacuaaccu uucguauacc auacuauauu gguccacuaa auccaagcua uaaugaagaa     1440

aaaaauuuua cuacuagcug gguaaaaaga cucgcaaaag ggaaggugac acccuggaac     1500

uucgaagaca uggucgauau ugaugcgagc cgcgaggcuu ucauagaaaa uaaacucaac     1560

aaauguagcu uccuccucga cgaaacagua uuaccaaaag acuccuuacu auaucaagaa     1620

uacaugguau uaaacgaacu aaauaauuua aaaauuaacg gucaaagcau caacaccgau     1680

cuuaaaaaau caauauauga agaacuuuuu aaaaaauaca agaaaguaac aagaaaacga     1740

cuuauuucuu acauaaaaga aaaagaaaau acuaguggag acauaguuau aacagguauc     1800

gauggagauu ucaaacaggg acuaaaaagc uacaucgcau uuaaagaaau aauaggcgaa     1860

aaaaucgaua augauaucua uagaaaagaa gucgaaaaaa uuauaagaga cauaucacug     1920

uacggggacg acaaaagcuu cauacagaag aagauaaaaa acgaaaacaa acuugaccua     1980

ucugacaacc aaauuagcaa aauagcaaag cuaaaguuca aagacugggg uagauucagc     2040

aaaaaacuac uaguaggaau caaagcaagc gacaaaaaca caggugaaau ugacaauauu     2100

auuggcuuca ugaggaauua uaauuauaau auaaugcaac uuauggcgaa uguuuucaca     2160

uucaaggaug aaauugaaaa acuaaauuca aaauauuaca cuuaugaaaa aaucaacgua     2220

gauuaucucg augaccuaua ucucucagcu cccgcaaaaa gaaugcuaug gcaaaguuuu     2280

caaaucguag augaaauaaa gaauauaaaa aacucugcac ccaaaaaaau auuuauugaa     2340

auggcaagau cuaaagaaga caaaccuaca agaaaaaugu cucgaaaaag cagauuagaa     2400

aaacuuuaua cagacagcaa aaaacaauuc aucaaacuau ucggucccga cgaauacgac     2460

aaacuaaaua acgaaauuaa aaacaaauca gaauccgauu ucagauggga caaucuauau     2520

cuauauuaca cacaguucgg cagaugcaug uauucauuag acccaauuga ucuagaagaa     2580

uuaucgaaca aaaaucuaua cgaucaagac cacauauacc ccaaauccaa aauauacgac     2640

gacaguauag aaaaucgugu auuaguuaaa aaacaacuaa aucuagauaa aucaaacgag     2700

uacccaaucc cagacagagu gcuaaacaaa gauaaaaaca aacuucucgc auucuggaaa     2760

augcugaacg agaaagaacu aauaggcgac aaaaaauacg cccgacuuau ccggaaaucg     2820

ccauuuacag acgaagaacu cggagauuuc auagcaagac aauuaguaga aacuagacaa     2880

gcaaccaaag aaacagcaaa caugcuuaga cgccucugcc cggacuccaa aauagucuac     2940

guaaaagcua agaauguuuc agacuuuaga cgaaaauucg auauauuaaa augcaggagc     3000

aucaacgauu uacaucacgc ucacgacgcc uacuuaaaca uaguuguagg uaacguuuuc     3060

gacacaaaau uuacaucaaa cccauuaaau uucaucaaaa acaacucaaa gaacagaagc     3120

uauaacuuag aaaaaauuua cgacuacgau guaauacgua acgaaaaaau cgcuuggcua     3180

gcggaaagaa aagaagaaaa ucaagauggc ucaauagcua cuguacuaaa aaaccugagc     3240

aagaaugaua uaagagucac gcggagaaca uacauaaaau gcgaagaucu auucgacgag     3300

aacauagucc guaaaggcaa cggaaaauuc ccacucaaag aaaacaacaa caaaucagau     3360

accaauaaau acggaggcua cucaggaaua aaaaacgcgu acuucguacu ccucgaagac     3420

gagaaacaca accgcauaau cgaagcaaua ccaucauuua uauacaaaaa agaaaaaaac     3480

ggagaaaaag guucugucau aaauuaccua caaaacauau uaggaauaaa aucagccaaa     3540

auauuaagag acaagauaaa aauaggauca cuauuaaaga uagauaaauu cccauacaau     3600

cuagcuggaa aaucaggaaa cggcauucua auuagcggag gaguacaacu aauacuagau     3660

aaaaacaccu caaaauacau uaagaaaauc gaaaauuaca aauacaaguu agacgaaaau     3720

aaagauuauc aaauaaacau auuugacaaa uuaaguaaag aagaaaacau uaagcucuuc     3780

gaccuauuug uagacaaauu ugagaauuca auuuauaaaa aucgcguaaa cauaaagcua     3840

aaagaaauua aacuugcccg aaguaaauuc gaacaccuau caauagagga acaaaucaaa     3900

acucuaacac aaaugcuaau acucuucggu cgagagaaca acgcgguaaa ucucgaauau     3960

auaggacucc cgaaaaauac ggggaaaaca aaaaucaaca aaaaaauaaa uaauaaagau     4020

aucaaaauua uaaaccaauc gauaaccgga uuauacgaaa cggaagaaga ccuagcaaau     4080

auauga                                                                4086


<210>  5388
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5388
augaaaaaac cauauagcau aggucuagau auaggaacaa acucagucgg augggcagua       60

aucacagaag acuacaaagu accagccaag aaaaugaagg uauuagguaa cacuucccga      120

gauuuuauua aaaaaaacuu auuaggugca cuauuauucg acagcggcga aaccgcagag      180

gccagaaggc uuaaacgaac cgcucgccga agauacacua gaagaagaaa ccgauuaagg      240

uaccuacaag agaucuucuu agaugagaug aacaaauuag acagcgguuu cuuucacaga      300

uuagacgaua guuuccuugu acccgaagaa aaaagaggcu ccagauaccc uauauuugga      360

acauuacaag aagaaguaaa uuaccacgaa aauuucccaa cuauauauca ucugagaaaa      420

aaacuagcag acuccaaaga aaaagcagau cugcgauuaa ucuacuuagc ccucgcccac      480

augauuaaau acagagguca uuuucuauau gaagagucau uugacuccaa caacaauagc      540

auacaagaaa uuuucaauga auuuacagcu gaauaccaua auaccauaga aggcucuugc      600

uugucgggcc agaucgaaca aguagaagca aucuuaacag auaagauuag uaaaucagcu      660

aaaagagaaa gaaucuuaaa acuuuuccca gcagagaaaa guacaggccu auucagugaa      720

uuucuuaagu ugauuguagg aaaccaagca gacuuuaaaa aacauuucga ccuagaagaa      780

aaaacaccgu uacaauucuc gaaagauacc uaugaagaag accuggaaaa uuuauuagaa      840

caaauaggug augaauucgc cgaucuuuuc cuauuggcua agaaacuuua cgacgcaaca      900

cuucuagcug gaaucuuaac agccacugac guauccacaa aagcaccacu uuccgccucc      960

augauuaaca gauaugaaac acaccaacaa gaccucgcag cacuuaagca guuuauaaaa     1020

aauaaacucc cugaaaaaua cuccgagguc uuuagugauc ugucaaaaga uggauacgcg     1080

ggauauauca acgggaagac gacgcaagaa gcguucuaca aauacauaaa aaacuuacua     1140

auuaaauucg agggggcugu agacuuucug gaaaaaauag aaagagaaga uuuuuuacga     1200

aaacaacgaa cuuucgacaa uggaaguaua ccacaucaac uacaccuaca agaaaugaac     1260

gcaauacuaa gacgacaggg ugaucauuac ccauuccuaa aggaaaaccg agaaaaaaua     1320

gaaaaaauuc uaaccuucag aauaccauac uacguuggcc cacucgcuag aggcaaucgc     1380

gauuucgcau ggcuaacacg aaacagugac caggcaaucc gaccguggaa uuuugaagaa     1440

gucguagaca aaaccucauc agcagaagac uuuauaaaca aaaugacaaa uuacgaucua     1500

uaucuuccag aagaaaaagu gcuaccaaaa cauucaauac uauaugaaac auucgcugua     1560

uacaaugagu uaacuaaagu caaauucaua gccgaaggcc uaagagacua ucaauuucua     1620

gacaguggac aaaaaaaaca aauaauuaau uuacucuuua aagauaaaag aaaagucaca     1680

gaaaaagaua uaauacacua ccuacauaca guggacggau augacggaau cgaauuaaaa     1740

gguauagaca aacaauuuaa cgcaucccuc ucaacauacc acgaucuucu aaaaauaaua     1800

aaagauaagg auuuuaugga ugaugcagac aacgaagcaa uacuagaaaa cauaguacac     1860

acauuaacga ucuucgaaga ucaggagaug auaaaacaau cacuagccca auacgaugcu     1920

auauuugaua gaaaaguaau gaaagcccuc acacgcaggc auuacacagg auggggagaa     1980

uuaagcgcaa aauuaauaaa cggcauuaga gacaaacaaa cuggaaaaau gauauuggaa     2040

uaccuuaaag augaugguga aauaaacaga aacuuuaugc aguuaaucaa cgacgauucg     2100

cuaucauuua aagaaacaau ucaaaaggcu caaguaaucg gcgaaacaga gaauaucaaa     2160

caaauaguac aagaacuccc uggaucaccu gccauaaaaa aaggaauacu ucaaucgauc     2220

aaaauaguag augaacuagu ugaaauaaug gguuauaauc cagaauccau aguaguagaa     2280

auggcaagag aaaaccaauu uacaaaccaa ggaagaagaa auucgcaaca acgacuuaaa     2340

aaacucacag acucgauaaa uaaacuaggc ucaaguauau uagaagaaca cccuguagau     2400

aacagucagu ugcagaacga uaggcucuuu uuauacuauc ugcaaaacgg aaaagacaug     2460

uacaccggag aagcgcuuga acucucaaaa uuaucgucgu augauguaga ucacauaaua     2520

ccucaagcuu ucauaaaaga cgacucauua gauaauagag uauuagucag uuacgcaaaa     2580

aauagaggga aaucagauga ugucccauca uuagaaaucg uccaaaaacg aaaagcauuu     2640

uggaggcaac uauuggacag ccaacuaauu uccgagcgca aauucaauaa ccucaccaaa     2700

gccgagagag gcggacuaac agaagacgac aaagcaggcu uuaucaaaag acaacuagua     2760

gaaacaaggc aaauaacaaa gcauguggcc caaaucuuag acgcacgauu caacacugaa     2820

gugacagaaa aaggcaaaaa aaaaagagcc guaaaaauaa uaacacuuaa aagcaacuua     2880

guaaguaauu uccgcaagga cuuugaacuc uacaaaguac gcgaaauaaa ugauuaccac     2940

caugcgcaug acgcauacuu aaaugccgua guaggaacag cacuccuaaa gaaauacccc     3000

aaacucgagc cagaguuugu uuacggagac uacccgaaau acaacuccua cagagagaga     3060

aaaacagcua ccgaaaaagu auauuucuac aguaauauaa ugaacuucuu caaaacuaca     3120

guaaaacucg cagauggcuc agaaaaaaaa agacaucaaa uagaaacuaa cgaagaaaca     3180

ggagaaaucg uaugggauaa aacaaaacau uucgaaauag ucagaaaagu uuuaucguau     3240

ccacaaguaa acaucguaaa aaagacggaa gagcagacug uuggccaaaa cgguggacuu     3300

uucgacaaca acauuguauc aaaaaauaag guaguagacg caaguaaacu aauaccaaua     3360

aaaucgggau uaucaccaga aaaauauggc ggcuacgcac ggccuacaau agcauacagu     3420

guccuuguaa uugcagacau agaaaaaggc aaagcaaaaa agcuaaaacg aauaaaagaa     3480

augguuggca ucacgauaca agacaaaaaa aaauucgagg caaacccuau ugcuuacuua     3540

gaagaaugug gauucaaaaa cauaaauucc aaccacauaa ucaaauuacc caaauacagc     3600

uuauuugaau uugacgacgg ucaaagaagg uuauuagcau ccucgauaga acuucaaaaa     3660

ggcaaugaau uaauacuacc uuaccaucuc acagcacuau uauaccaugc ccaacguaua     3720

aauaaaauuu cagaaccaau acauaaacaa uacguagaag cacaccaaaa cgaauuugaa     3780

gagcuucuaa cuacaauaau cucacuaagu aaaaaauaua uacaaaaacc uaacgucgaa     3840

agccuacucc aacaagccuu ugaccaaacu gacgaagaca ucuaccaguu aagcgaauca     3900

uuuauaucac uucuaaaauu aaucucauuc ggggcgccag gugccuuuaa guucuuaggg     3960

guagacauua gucaaucaaa cgugagauac caauccguga guucuuguuu uaacgcaacu     4020

cuaauccauc aaucaaucac aggauuauac gagacaagaa ucgaucuuuc uaaauuagga     4080

gaagacuga                                                             4089


<210>  5389
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5389
augaacgaac cguauggagu aggccuagac aucggcacaa auaguguggg uuggaccguc       60

guugauauga auggcagggu uagaaaaguc aaaggaaaaa cagcuuuggg ugcuagauua      120

uuuaaagaag gagcaacagc ggaggacagg agaggauucc gaacaacuag aagacgacua      180

aaaaggguua aguggagacu cagacuccuu agagaauuuu ucgaccaacc cauaucaaaa      240

auugacccaa acuuuuucgc aaggcguaag uauagcgaua uaagcccgcg ugauccaaau      300

uauaauggau uagccaaaac ccuguuuaau gaucgaacag acaaagaguu cuaugaugac      360

uaugcaacaa ucuaccaccu aagagauaaa uugaugacaa guaacagaaa guuugauaua      420

agagaaauau aucuugcaau acaucauaua gugaaauaca gagguaacuu uuuaagaaca      480

ggcccagcua gccaauaugg aucagcaucc cuccaucucg cuacauccuu ucaaaaacuu      540

aaugaucuuu ucgcccaguc agaagaaacu uuaaaucuaa aauugguaac cgaugaagcu      600

cuauuacaac aaauacagca aauauugguu aggacagauc uuuccagguc agaacagcaa      660

cggcaaauau ggccguuaau ggcaguacua accggagcca gugcagcuga aaagaaacgc      720

cagaaaaaug uagugguaga acuauccaaa gcgcuagugg gacucaaagc aaaaaugaau      780

guagucacac ucaccgaagu ugacgcagca guaguaaaag auuggacauu cacgauggaa      840

gaaucacaag acaaauuacc agaaaucgag gagcaacucu cagaaguagg acaacaaaua      900

auggaugaag uuauacaacu uuacgcaucc guuaauuuag cacaauugau uccagcagga      960

aagcgauuuu cacaacauau gguagaaaag uauaaacacc augaaaagaa ccucgaauua     1020

cucaaaacau acauucauuc gcaaagcgau aguaaaagag ggagagaaau aagagccaca     1080

uaugacaggu auauagacgg gguagacucc aaaccaguua cacaggaaau guuuuacaaa     1140

gaucuaauga aauacgucga ggcagaugcc acaucaaauc auuuagcagc cgaaauaaaa     1200

gacgagauag auagugaaca auuuaugccg aaacuacgca caaaacaaaa cgguucaaua     1260

ccauaucaag uucaacaaua ugaacucgau cagauaauca gucaucagaa gaaauacuau     1320

ccaugguuag gagaagaaaa uccagucgcc gaaagacgcg gaaaauuucc cuauaaauua     1380

gacgaauuag ugggcuuuag aguuccauau uauguaggac cauuaaucac aaaagaagac     1440

caacaggcca cgucuggagc cggauucgca uggaugguua gaaaagcaga uggaccaaua     1500

acaccaugga acuuugauca gaaaguagac agaaucgcau cagcaacugc auuuauucag     1560

cgaaugcaaa caacggacac auaccuaaua ggcgaagacg uuuuaccggc aagaucacuu     1620

auauaccaga gauuuauggu auuaaaugaa cuuaauaaca ugcgcgugga agauagaaaa     1680

cuagcucccc aacaaaaaca aagguuauau aaccaaguau ucaaacaaca ccaacacgua     1740

ucaguuaaaa acauucaaca aaaccucaug gaugcgggug aauauagaga ggcaccgaaa     1800

aucacaggcc uggcggaccc aaaaggauuu aauucaaguc ucagcaccua ccacgacuuc     1860

aaaaagauac ugccagaagc caucgcagau gaacauaaac gagcagauau agagaaaauu     1920

auuuuaugga gcacaaccuu cgaagauuca gcuauuuuua aacaaaaacu agaagaaguu     1980

gcauggcuaa cagaugcgca aagaaaacaa cuaucgggcu uaagauaccg uggaugggga     2040

caauuaucac auaaguuacu aauagcguuu aaagaugaua auggaagguc cauuauggau     2100

gggcuauggg aaacaucaga uaacuucaug caacuuagga aacaaccagc aauagaaacg     2160

caaauaaaag aagcaaacca ggcaaacuua acagaugcgg auauucagga uacuauuaau     2220

gaauuauaua caucaccgca aaacaagaaa gcgauaagag aaguaaugcu uguccuagac     2280

gauauuaaaa augcaaugca cgggcaaacu ccuucaugga uuuuuguaga agcugcaaga     2340

ggcggcggag ucgccggacg aagaacacaa aguaggucca gccaaaucgu agaagccuau     2400

aaaggaacag caaaagagau ugugucugaa aaaguucaac augaacuaaa ugagaaaauu     2460

aaagccaaag cagacuucaa uacgcgguua guucuauauu uucuacaaaa cgggcgagac     2520

cuuuacacca acgaagcaau aaacaucgau cggcuauccg aauacgacau agaucacaua     2580

uuaccucaaa gucuaguaaa agaugauuca cuagauaaca gagucuuaac cagugcaaga     2640

auaaauagag aaaagaacga uacuuuugcc agugaaaaau uuggaaggaa aaugggagcu     2700

caauggcgag aacuacaccg aaacggccua augacacaga gaaaacuuaa acacuuacua     2760

augagaccag acgaaauaag uaaacaugcg accgacuuca ucaaccgaca auuaguugaa     2820

acaagacaag uaauuaaauu aguagaggaa cuuauaaguu cugaauaucc ggcagcgucu     2880

aucgucgcag uaaaagcgaa ccuaacacac caauuucguc aaacuuuuaa cuuuccaaaa     2940

cuccgagaag uaaaugauua ccaucaugca uuugaugcuu cauuaacugc auuuauaggc     3000

auguaucuuc uaaaacaaua uccaaaauua gaaagauucu uuguauacgg gaaauuugcc     3060

aaacagccua uuaacuuaac uagauuuaau auaauuagaa aacuugcagu agcagaaaaa     3120

ccgauugcua auauagaaac uggagaaaua uuaugggaua aaacagccga uauuaaauau     3180

uucgaaaagc uauacaacua uaagagacua cuugugacac augaaguaag agaaaauuac     3240

ggagcaaugu ucaaacaaac uuuauuuaag gcuuccgaca acaaaagcaa aacgcuagua     3300

ccaaagaaaa aucauaugga gacauccguu uacggaggcu auagcaacca agagacggcu     3360

uaccuagcga uaguaagaau accauuuaaa ucgggcuuca aauucauagu aguagguaua     3420

ccaaccagaa ugguagcaaa aauuaaacau uaccaaaguu uaggagccac uuuaaaacaa     3480

gccacacaca aagucaucga accaaaauuu acuaaaauaa gucgaaaaac aaaacaaaca     3540

gugauaucgg auuacgaagu aguauugcca aaaguauacc uagaucaagu agucagagau     3600

caaguaaagg ggcaaaugua uagguucuca uuaggauccg acaaggaaua ucauaacguu     3660

caagaacuau acuuaccauu aucaauacaa caagcguuug uggggcacua cgacgaaagu     3720

gacgaccaac gaucuaacga cuuaguaaaa gucuaugacg ccgcauugaa acaacuacaa     3780

cgauauuuuc cccugcaucu aagucguaau uuugaucaag uagcaaauca agcucaucag     3840

ucauuugaag gucucaaaaa uaacgugcaa aaaucggaca aacaguuagg caagaaagaa     3900

guauuaaaca gccuauucgu aggacuacac gcuacugcaa cacgaaguaa ccuaucaguc     3960

cuaggaaugu cuaaagauuu cggacgcuug caaagcagau caggaauaac acucaccgau     4020

caagcagaga uaauauacca aucucccacu ggucuuuuug aacgaaaagu agcgcugaaa     4080

gacuuauga                                                             4089


<210>  5390
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5390
augacaaaac ucgguaagcc auacgggaua ggagucgaua uagggucuaa uucgauuggu       60

uuugcagcag uagacgaaaa uucccaucua auaagacuaa aagggaaaac cguaauuggc      120

gcaagacuau uugaagaagg caaggcggcc gcuaaaagaa gggcuggaag aacuacacga      180

cgaagauuau cacguaauag auggagacua ucauuuuuac gcgauuucuu cgaaucacac      240

auaaccccaa cagauccaaa uuucuucaug cgacaaaaau auucagaaau aucaccgaaa      300

gacaaagcuc gauauaaaua cgaaaagcga uuauuuaaug accgaacuga ugcagaauuu      360

uaucagcaau acucaacaau guaucauuua aggaaccguu uaaugacaga ucccucaaga      420

gcagauguaa gagaaauaua cuucgcuauu caucauauuc ucaaaagccg gggacacuuc      480

cuaacaccag gcgacgcaaa agauuuuaau acaaacgaug uagcguugga cgaaauauuc      540

ccagcauugc aagaugcgua ugcccaagua uaucccgacu uaggcauaac auuugacgaa      600

gacaaagcaa acgaguucaa aacuauacua cuaaaugaaa uagcgacauc cauagauacg      660

caaagagcau uagucaaacu acuacuagcg gaggaagaug acaaagacau acuuaaacag      720

caaaaacaag uauuaacgga guucgcaaaa gcuguaguag ggcuaaaaac aaaacuaaau      780

uuagcacuug guacagaagu ugacucauca gaagcaacag ccuggaacuu cucauuaggg      840

caacuagacg auaagugggc ggguauagaa ucagcuauga cagacgaagg uacagaaauu      900

cucgaucaga uucgagaucu cuauagagca agacucuuaa acggaaucgu accuacagga      960

aagacguuaa gccaagcaaa aguggacgac uacacacaac accacgagga ccuacaauua     1020

uucaaagcau accuaaaaca auuagaagac gacggaacag caaaagccau uagacaacua     1080

uacgaucgau acauagaugg agacgaugca gcacccuuuc ucagggaaaa uuuuguaaaa     1140

gcccuaacaa aagacgugac agcccacccu aacacuaaaa gucccgaguu acucgaaaga     1200

uuagcacaac cagacuucau gcuuaaacag cgcaauaaag caaacggagc aauaccagua     1260

caaaugcaac aaagagaauu agaccaaauu auuaagaauc aagcgguaua uuacgacugg     1320

cuagccgcac caaacccugu agagaagcac cguaaaagca ugccuuacca auuagaugaa     1380

cuucucaauu ucaggauacc uuauuacgua ggaccacucg uaacagcuaa agaacaaaag     1440

gcugcccaag gcggcgucuu ugccuggaug guccgaaagg aucccgaagg uaacauuaca     1500

ccauacaacu uugacgaaaa aguugacaga gaagcaucag cuaauacauu uauccaaaga     1560

augaaaacua cagacacgua cuuaauagga gaagacgugc uaccgaagca aucauuacua     1620

uaucaaaggu acgaaguccu uaaugaacuu aauaauguac gagucaacaa cgaaaaacuu     1680

ucaauugaac aaaaacaaca gguaauacgu gaacuauuug aacgacacaa caguguaaca     1740

aucaagcaau uugcagaaaa ucucagagca cacggcgauu augcacacau cccagagaua     1800

agggguuugg cugacgaaaa acgauuccua agcuccuuaa guacguauag acaguuaaaa     1860

ucgcuucucc cagaagcaau agaugaccca gccaaacaag cagacauuga aaauauaaua     1920

gcauggucaa cuguauuuga agacgcagca auuuucaaaa caaaacuaaa agaaauaucg     1980

uggcuuggua gcgaagcuau aaccaaacuc agcaacaucc gauacagagg auggggucaa     2040

uucucacgca aauucuuaaa cggacuaacu cuagggaaug ggcacacaau cauucaagaa     2100

uuacuucuaa gcacaaauaa uuuaaugcaa auucuaacag acgaaacacu ucaaaagaaa     2160

augacagagu uaaacgcuga uaaauuaaaa acagcgaaua uaaaugacgc gauagauaau     2220

gccuacacca gcccaucuaa caagaaagcc cugcgacaag ucuuaagagu uguugacgac     2280

auaaaaaggg cagcugacgg acaagaucca agcuggcucu auguggaaac cgcugacgga     2340

ggaggaacgc caggcaaaag aacgagagcu agacaacacc aacuacagga aauuuacgca     2400

aacgcagccc acgaacuaau agacacagca guaagaggug aauuagagga caaaauauca     2460

gacaaagcag acuuuaauga ucgccuugua cuauauuuua ugcaaggagg aagagacauu     2520

uauacaggcg caccacuuaa uauagaucag cugagcucau acgauauaga ccacauacuc     2580

ccccaaucau uaauaaaaga uaauucauua gauaacagag uauuaguaca cgccauaaua     2640

aaucgagaga aaaacgcaac uuucgcaucu acaauuuaug cccagaaaau gaaugccacc     2700

uggagacaau ggcaugaagc aggccuaaua uccggucgua aacuaagaaa ucuucaaaug     2760

agaccagacc aaauagacaa auaugcaucg ggauucguag caagacaacu uguugaaacu     2820

aggcaaauaa uaaaauuaac agaacaaaua gucgcagcac aauauccaga cacaaaaaua     2880

auugccguga aagcagguuu aagucaucaa uuaagagaag aauuagaauu ucccaaaaac     2940

cgagauguaa aucauuauca ucaugcauuc gacgcuuucc ucgcagcacg cauagguacg     3000

uaccuacuaa aacgauuucc caaucuacaa gcauucuuua cauauggaaa auuuaccaag     3060

gcagacguaa agaaauuacg aggcuuuaau uucauacgag acauuacgca ugccgaagac     3120

aaaauaguug ccaaagauac aggcgaaguu gucugggaua aacaacggga ugucgaugaa     3180

cuagaccgaa uauacaauuu caaacgaaug cucauaacuc augaaguccg auuugaaaca     3240

gcugaucuau ucaaacaaac uguauacggg gccagagaca gcaaggaagc aggaggaagu     3300

aaacaauuaa uaccaaagaa gaaaggcuac cccguagaca uuuauggagg auauuucaga     3360

gaaaauacug cauaucuagc cgucguaaaa gucacgaaga aaacagaaac aauauucaaa     3420

guaguuaaaa uugccacauc ccagguagcu gcauuaaaca aagcgagaag uagaucuaca     3480

gcagaagaau uaucagucuu aacagaauug cuaaaaccaa aauuuucaaa agucggcaaa     3540

aauggaaaaa uaacagacac accguuugaa guuguguuac ccagaguucc aagagagcaa     3600

uuauucuaua acgcuaaaua cggauucuuc augguaaaca gcgauacuau guuucauaau     3660

uuccaggaaa uuuggguauc aagaucagac cagaaaauau ugcaacaaau acgaaaagca     3720

aaaauagacu acccaaacgu agaucaagau uuggauaauc uuuuccauaa ccuagcagac     3780

caaauaguua aauacuucga uuuauacagc auugcaggau ucaaagagaa aaucagucaa     3840

agucaaaaca ccuuuaauga ucuagcagua gaugacaccg accaaagugu aggcaaaaua     3900

acgguaauua augaacuauu aaaaggagcu caagcaaacg gaaugacugg aucuuuaaaa     3960

guauuaaaaa uaucaacgcc uuucgguuuu acccaagaua aaucaggugu ucuaacaaaa     4020

gacaguagua uaauauauca aaguccaacg ggacuauuug aacguagcgu aagacuaaca     4080

gacuuauga                                                             4089


<210>  5391
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5391
augacaaaac ucggaaaacc uuauggaaua ggaguagaca ucggcagcaa cucuauagga       60

uuugcugcug uagaugaaaa cucucaccug auaaggcuaa aagguaaaac agugauuggu      120

gcacgacuau ucgaagaagg caaagcugca gcgaaaagac gcgcaggucg aacaacuaga      180

agaagguuaa gccguaacag auggagacug uccuuucuga gagauuucuu ugaaagucau      240

auaacaccaa ccgauccuaa cuucuuuaug agacaaaaau acucagaaau uucaccaaag      300

gacaaggcua gguauaaaua ugagaaaaga cuguuuaacg aucgaacaga ugccgaguuc      360

uaccagcagu auucuaccau guaucaucuu agaaauagac uaaugacuga cccgucacgu      420

gccgacguaa gagaaauuua uuuugcaaua caucacauau uaaaaucaag gggccauuuu      480

cuaacaccag gugacgcaaa agauuuuaau acgaacgacg uagcacuaga cgaaauuuuu      540

ccugcauuac aggaugccua cgcgcaagug uacccagauc uagggauaac auuugacgaa      600

gauaaagaaa acgaauuuaa aacaauauua cucaaugaaa uagcaacauc aauugauaca      660

caacgagcuc uaguaaaacu ccucuuagca gaagaagaug acaaggauau acuaaaacaa      720

caaaaacagg uguuaaccga auuugcuaaa gcagucguag gcuuaaaaac caaacugaac      780

cucgcacuug gaacugaagu cgacucuagc gaagcaacag cauggaauuu cucauuaggc      840

caacuagaug auaaaugggc uggaauagaa agcgccauga cagaugaggg aacagaaauu      900

uuagaccaaa uuagagauuu auaccgagca agacuucuaa auggcauagu ucccacaggc      960

aaaacccuau cucaggcaaa aguagacgac uauacacaac aucaugagga ucuacaacua     1020

uucaaagcau acuuaaaaca acuggaagau gaugguacug caaaagcaau ucgacaguua     1080

uaugacaggu acauagacgg cgaugaugca gcaccguucu uaagagaaaa cuuugucaag     1140

gcuuuaacaa aagaugucac agcacaucca aacaccaaaa guccggaacu uuuagaacgc     1200

uuagcacagc cugacuucau guuaaaacaa agaaacaaag ccaauggggc uauaccaguc     1260

caaaugcaac aaagagaauu ggaccaaaua aucaaaaacc aagcuguaua uuaugacugg     1320

cuagcagcac ccaauccagu agaaaaacau agaaaaucaa ugccuuauca acuugaugaa     1380

cuacuaaacu uccgaauacc auacuaugua gggccauuag uaaccgcaaa agaacaaaaa     1440

gcggcacaag gagguguauu cgcauggaug gugagaaaag auccagaagg uaauaucaca     1500

ccuuacaacu uugaugaaaa aguagauaga gaagcguccg caaacacguu uauacaaagg     1560

augaagacaa cggauaccua ucuaauaggc gaagauguuu ugccaaaaca gagcuugcug     1620

uaucaaagau acgagguacu uaacgaacua aauaauguaa gaguuaauaa cgaaaaacua     1680

ucuauagaac aaaaacaaca aguuauuaga gaauuauucg aaagacacaa cagcguaacc     1740

aucaagcaau uugcagaaaa uuuacgagca cacggagauu augcacacau accagaaaua     1800

agagguuuag cggaugagaa aagauuucua agcucacuau cgacuuauag acagcuaaaa     1860

uccuuacuac ccgaagcaau agaugauccg gcaaaacaag cagacauaga aaauauuauc     1920

gcauggagca caguauucga agacgccgca auauucaaaa ccaaacuaaa agaaauaagc     1980

uggcuaggaa gcgaagccau aacgaaacua ucaaauauaa gauauagagg gugggggcaa     2040

uucuccagga aauuuuuaaa cggauuaaca cuugguaacg gccauacaau aauacaagaa     2100

uuguuauuau caacgaauaa ucuaaugcaa auacuaacag augaaacauu acaaaagaaa     2160

augaccgaau uaaacgcaga uaaacuuaaa acagccaaua uuaaugaugc aauugauaau     2220

gcauauacgu cgccaucaaa uaagaaagca uuaagacaag ugcuacgagu gguugaugac     2280

auaaaaagag cagccgacgg ccaagacccc aguuggcuau auguagaaac ggcagacgga     2340

ggaggaacuc cgggaaaaag aacgagggcc agacaacauc aauuacaaga aauauacgcg     2400

aacgcggcac augaacuaau ugacacagcu guucggggag aacuagagga uaaaauuucc     2460

gauaaagcug auuuuaauga cagguuggua cuauauuuca ugcaaggggg uagagauaua     2520

uacacaggag caccacuaaa uauagaccaa uugagcucuu augacauuga ucacauacug     2580

ccucagaguu uaauaaaaga uaauucacua gauaaccgag uauuaguaca cgcuauaaua     2640

aaucgagaga aaaacgcaac auuugcauca acaauuuaug cacagaaaau gaaugcuacu     2700

uggagacaau ggcacgaagc uggauugauc agcgggcgaa aguuaagaaa uuuacaaaug     2760

cgaccagauc aaauugacaa auaugcaagc ggauuuguag ccagacaacu gguagaaacc     2820

agacaaauaa ucaaacugac cgaacaaauc guagcagcuc aauaucccga cacuaaaauc     2880

auugcaguua aagccgguuu aucacaccaa cuaagagaag aacuagaauu uccuaagaau     2940

agagauguua aucacuauca ucacgcuuuc gaugcauucc uugccgcacg uauuggaacu     3000

uaccuauuaa agagauuccc aaaccuacaa gccuucuuua cuuauggcaa auuuaccaag     3060

gcugauguaa agaaacuaag aggauucaac uuuauaaggg acauaacaca ugcagaagac     3120

aaaauaguag caaaagauac uggugaagug guaugggaca aacaaagaga cguagacgaa     3180

uuagaccgca uauauaauuu caaacgaaug uuaaucacac acgaaguacg auuugaaacg     3240

gcagaucuau uuaaacagac cgucuacggc gcaagagaua gcaaggaagc uggggguagu     3300

aagcagcuca uuccuaaaaa gaaaggauac ccuguagaca uuuacggagg auauuucaga     3360

gaaaauacag ccuaccuggc cguagugaaa gucacaaaga aaaccgaaac cauauuuaaa     3420

guaguaaaaa uugcuacaag ucaagucgca gcauugaaua aagcacgcag ccguucgacu     3480

gcugaagaac uaucaguauu aacugagcuc uuaaaaccaa aauuuaguaa aguaggcaaa     3540

aauggaaaaa uuaccgacac uccuuucgaa guaguauugc ccagaguacc aagggaacaa     3600

cuauuuuaua acgcaaaaua cgguuucuuc augguuaauu cagauacaau guuccacaau     3660

uuccaagaaa uauggguauc acguucagac cagaaaaucc uacaacaaau acgaaaagca     3720

aaaauagacu acccuaaugu agaucaggac uuagacaauc uauuccacaa ucuugcagau     3780

cagauaguaa aauacuucga ccuauacagc auugcggggu uuaaagagaa aauuucacaa     3840

ucucagaaca cauuuaauga ucuggcagua gacgauacag accaaagugu aggaaaaaua     3900

acagucauaa acgaauuacu aaaaggagcu caggcaaaug gaaugaccgg aucucuaaaa     3960

guucucaaaa uaagcacacc auucggcuuu acacaagaca aguccggcgu auuaacaaaa     4020

gauuccucca uaaucuacca aucgccaaca ggucuauuug aaagaucagu uagacucacg     4080

gaucuuuga                                                             4089


<210>  5392
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5392
augacaaaau uaggaaaacc cuacgggaua ggagucgaua uaggaucuaa uucuaucgga       60

uucgcagcug uagaugaaaa cucacacuua auaagacuua aaggaaaaac cguaauaggc      120

gcaagacuau ucgaagaagg uaaagcggca gcugacagac gagcaucuag aaccacgaga      180

agaagacugu caagaaaucg uuggcgacuu agcuuccuaa gagacuucuu ugaaucgcac      240

auuacgccaa cagauccaaa cuuuuucaug agacaaaaau auagugaaau uuccccaaaa      300

gauaaaucaa gauacaaaua cgaaaaacgu cuauucaaug acaggacaga ugcugaguuc      360

uacaaauacg aaaacugccc aacaauguau cacuuaagaa acagauuaau gacggauccu      420

ucaaaagccg auguaagaga aauauacuuc gcuauacauc acaucuugaa aagucgaggg      480

cauuuucuaa ccccaggaga cgccaaagau uucaacacaa auaaaguagc ggucaacaaa      540

auauucccag cauuacaaga cgcauaugca caaguuuauc ccgauuugga uauaacauuu      600

gaugaaaaca aaguaaauga auuuaaaaca guacuucuga acgaaaaagc uacaccuagu      660

gacacacagc gagcgcucgu uaauuuacua uuagcaaaag aaggagacag agauauucuc      720

aaacaacaaa aacaagcacu aacagaauuu gcaaaagcgg ugguaggcuu gaaaacaaaa      780

uuaaacguag cccuaggaac cgaagucgac uccucagaag ccacagcgug gaauuuuagu      840

cuaggacaac uggacgacaa auggacgggc auugagagcg cuaugacaga ugaaggaacg      900

gaaauacucg accaaaucag agaucuuuac agagcuagac uauuaaacgg aauaguacca      960

gcagguaaaa cacucaguca agcuaaagua gacgacuacg cacaacacca cgaagauuua     1020

gaauuauuua aagauuauuu aaaacaacua gaagacgaug gcacugcaaa agcuauacga     1080

cagcucuaug auagauauau agacggagau gacgcuaaac cauucgugcg ugaagacuuc     1140

gucaaagcau uaaccaaaga aguuacagcu cauccaaaug cgaaaagucc ugaauuacuc     1200

gaaaggcucg cucaaccaga uuuuaugcuu aaacaaagaa acaaagcaaa uggcgcaauu     1260

ccuguccaaa ugcaacagag agaacuagac caaauaauca aaaaccaauc aguauacuau     1320

gauugguugg cagcaccgaa uccaguagaa aaacaucgaa aaucuaugcc auaccaacuc     1380

gacgaacuac uaaauuuuag aauaccauac uaugucggac cccuaguaac agccaaagaa     1440

caaaaagccg cccaaggagg cguguuugca uggaugguaa gaaaagaccc ugacgguaac     1500

auaaccccgu auaacuucga ugaaaaagua gaccgagagg caucggcaaa caccuuuaua     1560

caacgaauga aaacaacaga uacuuacuua auaggugaag augucuuacc uaaacaaagu     1620

uugcuguacc aguccuauga aguacuaaac gaacuuaaua auguucgagu caacgauaau     1680

aaacuauccu ucgaucaaaa acaacaaguu uuaggagaau uguuugaacg ucacaauacg     1740

guaacaauaa aacaauuugu agacaauuua cucgcacacg gagauuacga ucgcauacca     1800

gaaguacgag gacuggcaga ugaaaaacga uuccugaguu cacuauccac cuacaaucaa     1860

cucaaaggca ucuugcauga agcuauugau gauagcgaua aacaaacgga caucgaaaac     1920

auuauagccu ggucuacaau auuugaagac aaagcuauuu uuaaaaccaa acuaaaugaa     1980

auaagcuggc uaaacgcagg aaugaucaac ucucuugcua acauuagaua uagggggugg     2040

ggacaauuua gucauaaauu auuaaacgaa cuaacacuau caacaggcag aacgguauuc     2100

gaagaacucu uaaucucuuc acauaacuua augcaaauuc uaacggauga aguacuaaaa     2160

cagaaaaucg cagaguuaaa cgcacaugaa cucaaggcgu cgggcaucua cgcggcaauu     2220

gaugacgccu acacaucgcc aucgaacaaa aaggcuuuaa gacaaguuuu aucagucaua     2280

gacgacauaa caaacgcagc aggccaagcg ccaaauuggc uauauauaga aaccgcagac     2340

ggugacggug uuccaggaaa acguacuaac aguaggcaaa gaaaaauaca agaaauauau     2400

gcuaacgcag cccaagaacu aauaaccgga ucggugagag gcgagcuaga agacaaaaua     2460

gccucucgag cagauuuuac agaccgauua guuuuauacu ucaugcaagg cggaagagau     2520

auauacacag gaaaaccacu uaauauagau aacuuaucaa guuaugauau ugaucauaua     2580

uuaccccaau cccuaauaaa ggauaacagu cuagauaaca gaguauuagu ucacccucua     2640

gucaauagag agaaagauac gauuuucgcu ucaacucgau uugccaaaca aaugaacgga     2700

auguggaggc aauggcaugc ugcaggucua auaaguucca gaaaacucag aaaccugcaa     2760

augagaccag augaaauaga uaaauaugca aguggauuug uagcacgaca acuugucgag     2820

accagacaaa uaaucaaacu gacagaacaa aucgucgcug aucaauaucc agauacaaaa     2880

aucaucgcag ucaaagcagg acucaguucu caguugagga aggaacuaga cuuuccaaag     2940

aauagggaag uaaaccacua ccaccaugca uucgaugcau uccuagccgc aagaaucgga     3000

acauaccugc uuaaacgaua ucccaauuug gaaccauucu uuacauaugg gaaauuuaag     3060

aaaacagagg uaaagaaauu aaaaucauuu aauuuuauac gagacaugac ucaugccaaa     3120

gauaaaauug uagcaaaaga aacuggagaa auuguauggg auaaugcauc ggacauaaac     3180

gaauuagacc gaauauauaa cuucaaaaga augcuuauca cucacgaagu acgauucgaa     3240

acagcaucac uauuuaaaca aacccuauac gccgcuaaaa auaguaaaaa ccgggguggc     3300

ucuagacaac uaauaccaaa gaagaaagga uaucuugucg auauauaugg aggguauaca     3360

caagaaacag ggaguuacuu aagcguggua cgauuaacua agaaagcaau guacgcaguu     3420

guaaaaguau ccacccggga cgcugcaaaa uuagcaguag caaaaucaau aucagaacaa     3480

aaagaaaaug aaacucucaa gaaaaucaua gacgggaaac uuucaaaaac aucuaagaaa     3540

ggaaaaacga cacaucaauu auuugaaauu guccugcccc guguaggcca gaaaacuuug     3600

uuuaaaaacu cuaaauauaa ccaauuucua guuaacagug auacauauau gcacaacuac     3660

caagaauuau ggaugccccg ugaauaucaa cguaugugga aagauauauu auuauccaac     3720

cauggcgacg cacaauuaga aggucaacuu gaucaaauau uuaaauuuau cgucagucaa     3780

guaaauuccu acuuuaaccu auaugauaua aaucaauucc ggaagaaaau agcagacgca     3840

acagauaaau uugcaaaauu accaacacac gauaccgaca augccagagg caaaaucgcu     3900

acaauaggac aauuguuaau cggucugcaa gcuaaugcua ugagaucaga ccuaagaaau     3960

uuagauauua aaacaccauu cggacuauua caaauuggaa cuggaauaac acuagaucua     4020

gacacaagca uaauauacca guccccgaca ggcuuauuug aaagagaggu accacuaacg     4080

gaccucuga                                                             4089


<210>  5393
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5393
augaaucaag aaccauaugc uauaggacuc gacauaggaa caucaucguu aggaugggca       60

aacgccgaua uggacgguua cauagcacgc guaaaagguc aaacagguuu aggcguaaga      120

cucuucgaag aaggccaacc cgcugcagau agacgcuccu ucagaacaac aaggagaaga      180

cuaucuagac gaagauggag auuaagguua cucagggaaa ucuucgauga accgauuucu      240

agcauugacc caaauuucuu cgcuagacaa aaggcuuccu caauaagucc uaaagacgac      300

agauucgaaa cuaccuauca gcuauucaac gauaaaacag accaagaguu cuauucaaaa      360

uacccaacga uauaccaucu aaggugggcg uuaaugacug aacacagaaa auuugaucua      420

agggaaauuu aucuugcggu acaucacauc guaaaauaua ggggaaauuu ucuaagaagc      480

ggaaacccua aaaguuuuaa accaagcgaa cuugacuuua gucaaagauu uaaagaaguu      540

aauggacuau ggcaccucau cuuugacgaa gucgcacccc aacugccuac agacaacuug      600

gaagaaauaa caaagcuagu acuagacaca acucggaccc gcgcagacag gcaaagagag      660

ucaguaaaau uacugauggc ugccacugac aaucacaaag acuggaaacc auauuuaacu      720

gaguucacca aagcaauauu aggacuaaaa accaaacuau acuugcuaac ggggguagau      780

gucgcaaaag cugaccagaa aacuuggaca uucagucuag aagacauuga agaccacaga      840

gcagacuugg aaaccgccuu ugaugauaga agccuagaau uaauagaaaa auuaguagau      900

cuccaagcug ccauccaauu aacugaaauu augccuagcg gacaauacuu cucgcaauca      960

augauauauc gcuaugauca acauaaagag caucuaagau gguuaaaaga uuacgcagaa     1020

caacaaaccg augcaaauaa acguaaagcg guaagacuag cauacgacca uuauauugaa     1080

ggagaugcca aaggaaaacc agaaauagcu gacgauuucu auaaagaacu caacagaaua     1140

cuuaaaggag acacauccga auuagcaaag aaaauaaauc acgaaauugc uuuagagcac     1200

uuuaugccaa aacaaagaac aaaagaaaac gguguuaucc cauaccaaau acaucaacaa     1260

gaauuagacu ccauaauaga uaaucaaaag aaauauuauc aauuuuuagg ggaggauaau     1320

ccaguagucu cacaccaugg aucgcaaccu uacaaaauag acgaauuagu uaguuucaga     1380

guaccuuauu auguaggacc gaugauaaca gcagaucaac aaaagaaaac ugcggcuggg     1440

caauuugccu ggaugguacg aaaagacagc ggggauauaa caccauggaa uuuugaugac     1500

aaaguagacc gaacggcuuc agcaacaaau uuuauacagc gcaugaaaac uacagauacg     1560

uacuuaauag gagaagacgu ucuacccuua caaucauuaa ccuaucaaaa auucauggua     1620

uuaaaugaau uaaauaacau ccgaaucaac ggagaauuac uaacaagaga acagaaacaa     1680

agaacuuaca augauguauu uaaaggacau ggcacaguca auguuaaaag uuugaucaaa     1740

agccucgaau gucacucuga aguuacagga gcaaucaaag uagaaggguu agccgacccg     1800

aaaagauuua auucagcccu uacaacauau agggauuuua agaaaauacu cccaguugca     1860

auagaucagc cacaaaggca agcagauaua gaaagaauaa uaaauuggag cacuauauuc     1920

gaagaugccg acaucuucaa agcuaaauua caagaaauag auuggcugac ugauacgcaa     1980

cgagauagac uaucaacuaa aagauaccga ggauggggac gucuguccgc uaaacuacug     2040

cuuaacauaa aagauaccca agggcuaucc aucauggacc aacuguggaa cacccaacau     2100

aauuucaugc aaauaguuca cgaaccagau uaugccgaga aaauugaaaa gaccaaugcg     2160

aaaggcuuaa aacaacaaga cauauucgaa acaauuaacg aaauguauac aaguccacag     2220

aauaagaaag cauuacguca aauucuugca guaguacgag acauccaaaa agcccaccau     2280

ggacaagcgc cuaguaggau auuuauagag gccgcgcgag gagcagagca aaauccacaa     2340

aggacccgac aaagacaaaa acagcuagcc gacuuguucg cagaucaggc aaaagaaaua     2400

guuaacgaaa ccgucgccca agaauuaaac gacgaaacag acaauaaagc aagauucacu     2460

gauagauuaa uguuauauuu cuuacaaaau ggucaagaua uguauacagg ugaaaaacua     2520

aacaucgaca aacuuucaac cuaugacaua gaccacauac uaccucaauc acuuguaaaa     2580

gaugauaguc uagacaauag aguuuuaguu ucaaaaguag aaaacuuaaa gaaaaacgau     2640

cauuucgcga gcgaacuaua ugcacgcaaa augcaaggca ccugggaauu auggagaucu     2700

gcaggccuaa uuagugcucg uaaacuuaaa caccuccuca ugcgcccgga cgaaauaaau     2760

aaauaugcaa cuggcuuuau aagaagacaa uuaguagaaa caagacaggu aauaaaacuu     2820

guuacagaug uccuaucaaa ugaauacgau agugacaaaa caaaaauaau aaguguaaaa     2880

gccggccuca gucaucaacu gcgcauggaa uuagaucucc caaaaguaag agaguuaaau     2940

gacuaucacc acgccuuuga cgcguuccua gcaguaagaa uuggaacaua ucuuuuaaaa     3000

cguuauccaa aauuggaaag guuuuucgua uacggacaau accaaguugc accacaauua     3060

gacuuacguc gauucaauuu cauacaugau cucguuagcg ggaaacaaga gaaaauaguc     3120

gcagaagaaa cgggagagau uguauggaau cgagaaaagg acguaucaua caugaaacau     3180

uuacuaucac uaaaacaccu uuuaguaacu caugaaguga aagaagauca cggagcccug     3240

uucaaccaaa caauauacaa agcgaagaaa gacaauggca auguaggagg uaaaaugaaa     3300

cuacuuccaa caaaaucuaa ucguccaaca gaaauauacg guggacauuc aucccaagcg     3360

auagcauauu uggcaauagu acgaguaaau ggaaaagaac caucguauag gguuaugggu     3420

guaccuauua gaauauuaga ucaacuaaaa caagcuaaga aacaagggga auaugcagaa     3480

agacuagcac ugaaacaauu cuuuacuccc gauuuuacaa aaggaaaagg caaaaucguc     3540

agugacuuug acguaguaau uccucauaua cccuuacuac aaccuguaau agaugaaauu     3600

ucugggcaau cacauagauu ugccuuaggg acaaauacuu acuaccgaaa ccugcaacaa     3660

cuaauacuca cuuuagcaga ucagaaagua uuaauaucua aagaaacugg uguugacgaa     3720

gaaaagaaac ucguagaagu auauagucau aucugccaac aaguaaugaa auuuuuccca     3780

cuauacgaca ggaauaaauu cagagaacug cuaguugauu cacgaucaca cuuugaacag     3840

cuaccgauag uaaacagaua cucugacgcu ggaaaacuag aaauagcagg uaaacgcgga     3900

auacuucaac aaaucauggu aggucuccau gcuaaugcaa caccuggaga cuuaaaagaa     3960

cuaggaguca aaacucccuu cggaaaacua caaguaccgg gaggaauaaa acuuacugaa     4020

aaugccaaac uaaucuauga gagcccaaca ggacuuuuua aacgagaagu aaaaauaaca     4080

accgaauga                                                             4089


<210>  5394
<211>  4089
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5394
augaaaaaga aagcagaaaa gauauauuug gggcucaacg uaggaacaaa cuccauagga       60

uacgcgguua ccgaugaaca guacagacug uuaaaauuua auggaaauga ugcauggggc      120

ucaauaguau uugaugcugc cagcucaaau gaagaucgac gcaaucaccg auuggcgcgc      180

agaaaucuag accgaagaca acaacgcaua guuuggcuac aagaaaucuu cgcaaaagaa      240

auaagcaaag ucgaugacag guucuuuauu agacuaucag aaaguuuuag auggagagaa      300

gauguacaag aucgcuaugu cuuuuucaau gauaaagaau acacagacgu ucaauauaug      360

aaagaauauc cuacgauaca ucaucuaaua ugugaacuga uggauaacaa aaccccacac      420

gauguucggc ucguauaucu ugcaugugca uggcucauca cucacagagg ccauuuccug      480

aauaauuuaa augucgaaaa acuugacgaa auaauggacu ucucaagugu auaucaaaac      540

uuuauuaacu auuuuacuga aaacggauau aaaagaccuu ggggugaaau agaugugagu      600

gcacuuucag aaguacuaaa acagaaaacu ggcaucaccg ucaaaaacaa agaacuacaa      660

aauauacuac uggaagggaa aaagccagaa aaaucaggaa cagaagaauu uccguuuucu      720

caagauucua ucauacgucu guuagcuggg ggacaaugca aauuaaagga uguauuuugc      780

aaagaagaau acuuagauuu gggaucagua agccugggua uggacgaaga aaaauuugac      840

gaaauaucag gaaguauagg agaagauuac gaccuucuca uugcccuaag ggguuuauau      900

gauuggucgg uacuugcaaa ucugcuaaac ggccacucag aaacaauuag cauggcgaaa      960

guacaaaugu augaacaaca cggaaaagau uugcaaauau uaaaguacuu cgugcgaaaa     1020

uacguacccg acaaguauaa ugaaguuuuu cgaaaagcaa aagcugauaa cuacguugca     1080

uacacaggac auauggauaa agacacagcc agccaaauua aaaagaaagc caaagcagaa     1140

gaauuuucua aauauguacu uaaacuuaug agccccauua aaccggagaa aacagaccug     1200

caaucguacg aagacaugug cgaaagguua aaauuaaaca gauuccuucc aaaacagaaa     1260

aauacagaua acagaguuau uccacaucaa uuauaugaau augaguuaau ucaaauucua     1320

aagaacgcau cuguuuauuu accauuccuc aacgauacca auaaugguau aagugaagcg     1380

gacaaaguac ucucggucuu uagauacaaa auaccauacu acguaggacc auuaaacgaa     1440

gaaucuuccu uugcauggau cagcagaaaa caaggaaaaa uaaccccaug gaauuuuaaa     1500

gagaugguag augaagaugc aagcgaaaau gcauucauaa gaagaaugac aaacaaaugu     1560

acuuaccucc caacggaaga cguacuaccg aaagacaguc uauguuacca gaaauuuaug     1620

guacucaacg aaauaaauaa ccuccaaauc gacggacuca aaauuccagu aaaagcaaaa     1680

caagggauau accacgaacu auuugagaaa aacaagaaag uaaaacguaa agauauagaa     1740

gaauacuuaa uuagaaacaa cuacuuagac aaagauagua ccgaacuaau aucaggaaua     1800

gacgaacaaa uacacagcgg acuauccuca uacugcgcau ucuuuaauuu auuaaauaag     1860

aaaauacucu cagaagauga cguggaacga auaauugaac gugccucaua cgcagaagau     1920

aaaucaagag uaaucaaaug guuaagaaaa gaauaccccg gacuaucaga agacgauauu     1980

cgcuauauug ccagaaucaa aauuagagac uucggaagac uaagcaaaac uuucuuaaca     2040

ggauuaaaag gaugcgaugu uuccaaagga uuugauggac aagauguaga aaugauaagu     2100

auauuacaaa caauguggga aaccaaugau aauuuaaugg aaauucuuuc agacaaauau     2160

acauuucuag aagccuugga agcauucacc caagaauauu acacggguaa aaagaaaucu     2220

cuaaaagaaa gauuagacga aaugagagug ucaaauucag uaaggagacc aguauaucgc     2280

acauuagcug uagucaaaga uauagaaaaa gcguuuggca aaccaaacaa aauauucguc     2340

gaaaugacaa gagggggaca ggcagaccaa aaaggcaaac guauaaaauc aagaaaacaa     2400

cagauuuuag accucuacga uaaauguaaa gaugaugucc augacuuaau guggcaauua     2460

gagaguuuag gagaauaugc agacucuaga cuacaaucgg auaggcucua uuuauacuuu     2520

augcaauucg gaaaaugugc auauucagga gcgcauaucg guuuugaaca acuuauggcc     2580

ggguccguag aauacgacau agaucauauu uauccacaaa gcuauguaaa agaugauuca     2640

cuuaauaaua aaguucuugu auuaucagau uuaaacggga cgaaagacaa uaucuacccc     2700

gugagcccag aaaucagggg aaaaaugcaa ggauuaugga gcugguggaa ucauguagga     2760

gccauaagug augaaaaaua caaaaggcuu auaaggucag caccauucac cgacgaagag     2820

aaaaugggau uuaucaacag acaauuaaca gaaaccucac aaucaacaaa auuuauugca     2880

gacuuauuaa aagaaagauu cccagacgca gaaguaguau acguaaaagc cggccuagua     2940

agugauuuca gacacgaauu ugacuuaccu aaaucaagaa guuacaacga ucuccacaau     3000

gcagcagacg cauuucuauc aguuguugcc ggaaacguau augauaugag auucuccaaa     3060

aagugguuuc gcauagacga aaaauauuca guaaagguaa aaacaauauu uacucaugaa     3120

guaaaaugua ggggagacau uauuugggac ggaaugccaa uguuagaaaa aguugaagau     3180

acugcuagga agaacacagc acauuucgua aaauaugcag ccuucaagac uggcgguuug     3240

uuugaccaaa auccggucaa gaaaggaaca gggcugauac cacuaaaagc uggccugccu     3300

acagaaaaau augguggcua caauaaagca gguauaaugu uucuaauccc aguaagauau     3360

aauauuggca agaaauccga aauacuuauu cuaccgguag aauuaaugca uggaaaacac     3420

uucuuagaag acaaaguauu ugccagaaac uacacauuua cacguuuaga aaacauauua     3480

ggaaagaaag uuggagaagu cucguucccu auggguauga ggccauggaa aauuaauaca     3540

auguuaucau ucgauggauu cagaguaugu uuaacaggca caucaggcag gggaaaaugu     3600

cuaguagccc aaccgguaau gcaauucagu gcagacgagc auuggagauu cuauguaaaa     3660

cgauuagaga gauucgugga gaaaaucgga auaaauccag auuuacuaua cgauaaagac     3720

caugacaaag ucuccaaaga ugaaaacuua aaacuauaug aacucuacgu agauaaacuu     3780

caaaacacga uauauaagaa acgaguaaac agcccagucc aaacccuacu caaaggaaaa     3840

gaagaauuua agaaauuauc uauugaggaa caaugccaag uauuacuaaa uauccaaagu     3900

guuuuugguc gcaugacugg aggaugcgac cugacacuaa uaggaggauc ugcucacucc     3960

gcagcaaccg guaguuuauc aaccacaaua uccaacugga aaaagaaaua uuccacuguu     4020

agaauuaucg accaaucccc cucagggaug ugggaaaaau cuagugaaaa ucuauuagac     4080

uuuuuauga                                                             4089


<210>  5395
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5395
augaccaaau uaggaaaacc auaugguaua ggagucgaca uuggcucuaa cucuaucggc       60

uucgcagcag uugacgaaaa cucacaccuu auucgacuaa aaggaaaaac ggucauagga      120

gccagacuuu ucgaagaagg aaaagcagcu gcagaccgac gcgccucacg aacaacaagg      180

agaaggcuaa gcagaaacag auggagacua ucuuuccuuc gcgacuuuuu cgaaagccau      240

auuacaccca cagaucccaa cuuuuucaug agacaaaaau auucagaaau aucaccuaaa      300

gauaaggcac gauacaaaua cgagaaaagg uuauuuaaug accgaacaga cgcggaguuc      360

uaccaacaau acccaacaau guaccaucuc aggaaucguc uacucacaga uccaaguaaa      420

gccgauguac gagaaauaua uuucgcuauu caucauauac uaaagucucg cggacauuuc      480

uuaacaucag guuacgcaaa agauuucaau acaaauaagg uugcacuaaa ugaaaucuuu      540

ccggcauuac aagacgcuua cgcacaagua uacccagacu uagauaucac auucgaugaa      600

aacaaaguua augaauuuaa aaccguacua cuagacgaaa aggcuacacc gagcgauaca      660

caacgcgcgc uaguaaauuu gcuacuugca gaagacggug auaaagauau acucaaacaa      720

caaaaacaag uacugacaga auuugcuaaa gccguuguag gccugaaaau gaaacuaaac      780

guagcuuuag guacugaagu cgacucuucu gaagcaaccg cuuggaacuu cuccuuaggu      840

cagcucgacg acaagugggc aggaauagaa ucagcaauga cagacgaagg gaccgaaauu      900

cucgaccaaa uaagggacuu auacagagca cgacuguuaa acgggauagu uccagcaggc      960

aaaacauuaa gccaagccaa aguagacgac uaugaacaac accaugaaga ucuugaacuu     1020

uuuaaagacu acuuaaagca acuagaggau gauggaaugg caaaaguaau aaggcaacuc     1080

uacgaucguu auauagaugg agacgacgca aaaccauucg uucgcgaaga cuuuguaaaa     1140

gcacuuacua aagaagucac ugcacauccg aaugccaaaa guccggaacu acuugaacga     1200

uuaguucaac cagauuucau gcuuaaacaa agaaauaagg cgaauggcgc cauaccggua     1260

caaaugcaac agagagaacu ugaucagaua aucaaaaauc aauccgugua uuacgacugg     1320

cucgcagcac ccaauccagu agaaaaacac agacaaagca ugccauacca auuagacgaa     1380

cuucuaaauu ucaggauacc uuacuaugua gguccauuag uaaccgcaaa agaacaaaaa     1440

gcagcucaag guggagucuu ugcuuggaug guaagaaaag acccagacgg aaauauaaca     1500

ccauacaacu ucgaugaaaa aguagauaga gaggcuucag cuaacacuuu uauucaaaga     1560

augaaaacaa cagauacaua ccucauagga gaagacgucc uaccaaaaca gagucuccuu     1620

uaucaaucau augaaguauu aaaugaacua aacaaugucc guguuaacga uaacaaacua     1680

ucauucgauc aaaaacaaca aguucucggu gaacuauuug aaagacacaa uacaguuacu     1740

auaaagcaau ucgucgacaa cuuauuagca cacggugacu augauagaau cccagaaguc     1800

cgagguuuag ccgaugaaaa acgauuccua ucaagccuau cuaccuauaa ccaacuaaaa     1860

ggaauacucc augaagcaau agacgacagc gacaaacaaa cggacauaga gaaaauaauc     1920

gcauggucaa caauauuuga agauaaagcg auauucaaaa cuaaacuaaa ugaaauuuca     1980

uggcucacag ccgggaugau aaacuccuua gcaaacauua gauauagagg uuggggucaa     2040

uuuucucaua aacuucugaa cgaauuaaca cuuuccacag gaagaacugu auucgaagaa     2100

cuucucauau cuucucacaa cuuaaugcaa aucuuaacag acgaaguacu gaaacagaaa     2160

auagcagaac ucaacgccca cgaacucaaa gcaucgggca uauaugcagc aauagaugac     2220

gcauauacgu cuccuuccaa uaagaaagca uugcgacaag uacucucagu aauagacgau     2280

auaaccaaug cagccggaca agcuccaaac uggcucuaua uugaaacagc cgauggagau     2340

ggaguuccag gaaaaagaac aaacucaagg caaagaaaaa uacaagagau cuacgccaau     2400

gccgcccaag aguuaauaac agggaguguu cguggcgagc ucgaagacaa aauagccagu     2460

agagcagauu ucacagauag auuagugcua uacuuuaugc aagguggaag agacauauau     2520

acaggugaac cacuaaacau agauaacuua agcucauaug acauagauca cauacuaccg     2580

caaucucuaa uaaaagauaa uagccuggac aaccgagucc uagugcaucc ccuagucaac     2640

cgcgaaaaag acacuauauu ugcuucaaca agauucgcaa aacaaaugaa cggaaugugg     2700

cgacaguggc augcggcagg auuaaucuca ucccgaaaau uacgaaaucu ucaaaugaga     2760

ccagaugaaa ucgacaaaua cgcaucggga uucguugcac gacagcuagu cgaaaccaga     2820

caaauaauaa aguuaacaga acaaauaguc gccgaccaau acccagacgc uaaaaucauu     2880

gcaguuaaag caggauuauc aucacaauua cgaaaagaac uagauuuucc caaaaaucga     2940

gaagucaacc acuaucacca cgccuucgau gcauuccuag cagcaagaau aggcacauau     3000

cuccuaaaac gauacccaaa ccucgaacca uucuucacuu auggaaaauu uaagaaaacc     3060

gaaguaaaga aauuaaaauc auuaaaauca uucaauuuua uccgcgacau gacucacgca     3120

aaagacaaaa uaguagcaaa agagacugga gaaauaguau gggacaacgc uucagauauu     3180

aaugaacuag aucggaucua caacuucaaa aggaugcuaa uaacccauga ggucagauuc     3240

gaaaccgcua gccuuuucaa acaaacauua uacgccgcua aaaauuccaa aaacagaggu     3300

ggcagcagac aacugauacc gaaaaagaaa ggauaucuag uggacaucua uggaggauau     3360

acucaagaaa cagggucaua ccuaucaguc guacgguuga ccaagaaagc aauguaugca     3420

gucguuaaag uaucuacaag agaugcugca aaacuagccg uagcaaaaag uaucuccgaa     3480

caaaaagaga acgaaacauu aaagaaaaua auugauggaa aacuaucuaa aacuuccaag     3540

aaaggaaaaa caacacauca auuauucgaa auaguucuuc cacgaguagg ucagaaaaca     3600

cuauuuaaaa auucuaaaua caaccaauuu cuaguaaaca gugauacaua uaugcauaac     3660

uaccaagaau uauggaugcc ucgagaguac caaagaaugu ggaaagauau acuacuauca     3720

aaccaugggg augcacaacu agaaggacaa cuagaucaaa uauucaaauu cauaguuuca     3780

caagucaauu cauacuuuaa ccuauacgac aucaaccaau uccgaaagaa aauagccgau     3840

gccacugaua aauuugcuaa acuacccacc caugacacag auaacgcaag aggaaagauc     3900

gcaacaaucg gacaauuacu cauaggauua caagcgaaug caaugcguuc agaucucaga     3960

aacuuagaca uaaaaacacc auucggucua uuacaaauag ggacaggcau aacauuagau     4020

cuagauacaa guauuaucua ucaaucaccu acuggcuuau ucgaaaggga aguaccuuua     4080

acugaucuau ga                                                         4092


<210>  5396
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5396
augaggggga acuaccauau aggucuagac auagguacaa guucaauugg augggcaguu       60

auugacgaca augcaaaagu gaugcgagca aaaggaaaaa cagcaauagg cgcuagacua      120

uacaacgaag gacaaacagc agcagauagg aggcucuuua gaacaaccag aaggcgacuu      180

aacagacgaa aauggcgauu aaaacuacug gacgaaaucu uugacccuua uauaaccccu      240

gucgaugccg cauuuuucgc acgucuuaaa caaucuaauu uaagcccuaa agaccaaaau      300

agagauugga cagguucaau guuauuucca gaccguacug auagaaaauu ucacaaagau      360

uacccaacua uuuaccacuu aagacauaag cuaaugaccg aagaucacca guuugacaua      420

cgagaaauau aucuagcaau ucaucauaua guaaaauaca gaggcaacuu ccuaaacacc      480

acaccgguuu caucauucag cguuagugau guaaaguuug augaacaauu cgacagacua      540

aacgaauuau augcagcugu aucuccugac gaacccuucg caaucaaugg cgauaacucu      600

aaagcgauag aaacacuacu auuauccgac gaaacuaaaa aauuugauaa acaaaaacaa      660

ucugcaaaac uuauccuauc agaaacaggg gacaaagcuu uagauaaaga gaauaccaaa      720

guggcgacuc agaucucuaa agcaaucuua ggauacaaaa cacaacuuga cguuuuacuu      780

aaauacgacg caauuaacag uucagcaugg aagaucgaau uagacgacga aaacauagau      840

gauacccuac caggacuacu gucccaacug gaugaaaauc gacaagaaau gguaagcaua      900

auccaaaacu uauaugccca gguaacgcuu aaccaaauag ucccaaacgg caugucauua      960

agugagagua ugauagagaa auauaaugca cacagagacc aucuaaaacu cuuaaagcaa     1020

cuagaagaua aaauagacga uccaaagaaa aaaaaagguu uagaagcagc auaugacgca     1080

uacguuggua agaacaugaa aguagccaau caagauguau uuuacgcauc aguuaagaaa     1140

aaucucgacg acagugaccu agcaaaacaa auagcagacc uaauagaggc acaaaaauuc     1200

augccaaaac aacgcacaag ccaaaaugga guuauaccac accaacuuca ccaauuagaa     1260

uuagaucaaa uuaucgaaaa ccaaucaaaa uacuauccau ggcuaacagc agcaaauccg     1320

aacccgaaac gaauacauca agcuaaauac aaacuagaug aacuaguagc auuuagaaua     1380

cccuacuacg uaggaccacu aguaacgccu gaagaacaag aaaaaucugc aaaccaagug     1440

uucgcaugga ugaaaagaaa acaaccagga accaucacac cuuggaacuu ugaucaaaaa     1500

guugaccgaa uggcuucagc aaaucgcuuc auaagacgaa ugacuacaaa agauacguau     1560

cuuauuggcg aagacguauu accagacgcu ucacuaauuu augaacgguu caagguacua     1620

aacgaauuaa accuagugaa agcaaacuau caccguuuag caccagcgca aaagcaggca     1680

gcauaucgag aucuauuuga aaaccaaaaa agcauuucaa caaagaaauu acaaaacuau     1740

cuaguagacc aaggauauuu cacagcaaaa cccaagauaa caggguuaag ugacccagaa     1800

cgcuuuaaua gcucauuagg gacauauauu gacuucaaag aaauuuuugg ugaucaaguc     1860

gauaacccug accgacagga ugauuaugaa aaaauaauag aauggaguac agucuuugaa     1920

gauagacaaa uuuaugaagc caaacuacgu acccuagauu ggcucacuga agaccaaaua     1980

caagcccuaa agacaaaaag auauaacgga uggggacgcu uaucuaaaaa acuacuaaca     2040

ggaaucguag augaaaaugg agaacgcauc aucgaugucu uguggaacac uaaccaaaac     2100

uuuaugcaag uacagucaca agaaucauuc gccaaaagaa uacaugaagc caacgcuaaa     2160

caaaugaagg uaacgggagu agaagacaua cuagcugacg cauacacauc uccacaaaac     2220

aagaaagcaa uucgucaagu aauacggguu guagaugaua uacaaaaagc aaugggcgga     2280

guggcuccaa caucaguaag cauugaguuu acacgcagcg aagaccaaaa ucccaggcga     2340

acacaaucaa gagaaucuca acuacagaau acuuucaaag uagacgccaa agaaaucgua     2400

gacucagacc ucgugacuga acuagaaacc guuucaaaau caaaaaaagg ucuaacagau     2460

cgauuauauc uauauuucac acaguuagga aaagauaugu auacuggcga cccauuaaac     2520

aucgaugagc uaagcacaua ugauauugau cauauccuac cacaagcguu uaucaaagac     2580

gacagucuag auaacagagu cuuaguuagc aaggccguaa auaacggaaa aagcaauaau     2640

guaccaguua aacuguuugg aggaaaaaug gggggauuuu ggaaagaacu agcagaucau     2700

agauuaauaa guaagagaaa auuaaaacac cucuuaacag auccagauac cauagauaaa     2760

uacacaauga gaggauuuau cagacgccaa cuaguagaaa ccagccaagu aauuaaauua     2820

guggcucaga uauuagggga caaauaccaa aacgaugaua caaagauuau agaggugaca     2880

gccaagauga aucaccaaau gagagaaaau uucaagcuca ucaaaaaucg ggaaguaaau     2940

gacuaucacc acgcaguaga cgcguaccua acagcguucg uaggacaaua uuuauauaaa     3000

agauauccua agcucagacc auacuucguc uauggcaacu ucaaaaaauu caacgaggac     3060

caugucagcu uucgcuauuu caauuuccua cacgaauuaa uggacgagaa caauaaaaga     3120

auuguggaac cugaaaccgg agaagugguu cucgaacgag aaccugcgau agcauauaug     3180

aaacgaguau aucacuucaa guucaugcua aucucacaag agguauacac cagacgcgau     3240

gcaauguuca accagacaau cuacccagcc aaagaagcaa auagaagaaa auuaauagca     3300

aucaagcaug gaaaaccagu agauguauac gguggauaca guggcucggu agaugcauau     3360

auggccaucg uacguauaca cgauaaaaaa ggagacaaau acaaaguugu cggaguuccu     3420

augagagcau uagaaauacu uaacgcaaca cacgauaaaa aagauuauga ugcaaaacua     3480

aaacagauac uaguaccaaa auucaccaaa guaaaaaaau caagaaaaac uggugagaua     3540

accaaaaagg uggaagacuu ugaaauagua cucgggaaag uaauguaucg acagcuaguc     3600

auagacggag auaaaaaauu caugguugga ucuuccacuu accuauauaa cgccagacaa     3660

cucgugcuaa gcgauucauc ccuagacaca uuggcaucca agggaacguu agauagugac     3720

gccgacucca aaagauuagu cgcgguauac gaugaaaucg uagaaaaggu agauaaauau     3780

uucccauuau acgauaaaaa ugcuuuucgu aguggauuac acaaaggccg agauaaguac     3840

uuguuauuac caaaccgauc caccuuuaau ggagcaaaaa aaguacauuc uggcaaacgc     3900

gaaauccuga augagauacu aaacgguuua caugcaaacg caacacuugg aggauuaaaa     3960

gaucuuaaaa uaucuacacc auucgguaaa augcaagggc aaucaggcau uccauuaucu     4020

aguaacacaa uaauauguua ccaaucacca acaggccuau ucgaacaucg aguaaguuua     4080

aaagaucuau ga                                                         4092


<210>  5397
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5397
auggaaaaca accgauauaa cguaggauua gauauuggaa cgucauccau cgguuuugca       60

guaacagaug aacaagacga acugguucaa guaaaaggca aaaauuacau ugguguaaga      120

cuauucaaag aaggucaaac cgcgucugaa agaagaucau uuagaacaac cagaagacgg      180

cuaaaaagaa gaaaauggag auuaggucua cuugaagaaa uauuugcccc auaccuagca      240

caaggggauc cauauuuccu ugcaaggaug aaagagucaa acaucagucc acgugaugaa      300

cgcaagaaau acuacggauc cuuauuauuc agcgaugaca ccguauuuua ugacaaauac      360

ccaacaguau aucaccuaag acaugcacua augacugaag acagaaaauu ugacauuaga      420

gaaauauauc uagcgguaca ucacauaauu aaauaccgag gaaacuuucu aauaaacacc      480

cuaauuaacg aguucucauc aaaagcagua gauuucaaga aagaugcaga cauacuaaau      540

gaccucuuug aacaauucua cauuagugau auuaaugauu cguuucagcu caacacggca      600

gauaugaacg aaauauccga acuacuccuc gacaacagca aaacacgacu agacagacaa      660

cgagaaagcc ucaagaaacu agccaagaaa guagacgaca uuaguguagc gaagaaacau      720

cagaaaauug caacagaauu uaguaaagcc auauuaggaa acaaagccaa auucgacgua      780

auucugaaug uaacgacaga cucaucaaau gauuggucgu uuaaacuaga agaugaagac      840

gcugaugaga aaauuguagu cuuauuagaa gaacuaaaua cuccgcaaca agaagcucua      900

aacauaauac aucaauggua cucacaaauc ucacuaaacg gaauaguacc agacggcaaa      960

aguauuucag aagcaaugau uagcuccuac gaacuacaua aagaacaccu agcucuauua     1020

aaacaacaaa uaaacaacac caaagacaaa aagaaagccc acggacuaca ugaagccuau     1080

aacgaauaug uacacggcaa caacaauaag aaacuaaacc aagcagauuu cuacuccaaa     1140

guaaaacaaa auuuggaauc uucacuuaua acggacgaaa uacucgaacu aauuaaugcg     1200

gaaaacuuca ugccaaaaca acgcacaucu gcuaauggag ucauaccaca ccaacuccac     1260

caacaagaau uagacagaau aauacaaaau caagcuaaau acuauccauu ccucgccgaa     1320

acaaauccaa acgagaaaag aagcaaaguu gcaaaauaca aacucgacga auugguagca     1380

uucagaguac cauacuacgu cggaccccua auaacggcaa cugaccaaaa gaaaacaucu     1440

ggaaaagaau uugccuggau gguucgaaaa caaccaggac gcaucacacc auggaauuuu     1500

gacgaaaaag uaaauaaaau ggaaagcgcc aacacuuuca uaaggaggau gacaacaaaa     1560

gacucuuacc uaauagccga agauguacuc ccagaugaau cccucuuaua ucaaaaauuu     1620

aaaguccuca acgaacuaaa uaauuuacga gcagacggac guaaauuaaa agucaguguu     1680

aaacaaaaag uuuuuaaaga auuauuucag caacagaaaa ccauuacuau agucaaacuc     1740

caaaauuauu uaaaagcaga auguucuuuc cuaacgacac cagaaaucuc cggucuuagu     1800

aaaccugaac acuucguuag uucacucgca acauacauag acuuaaagaa aauauuugga     1860

gaggacaucg auaacccugc uaggcaaaac gauuuugaac gcaugauaga augggcaaca     1920

auauucgaag acaaaaauau auacucagaa aaacucaacg agauaaaaug guuaaacgac     1980

cgacagaaga aagaacugaa aaauaaaaac uaucacgggu ggggaaaauu aagcgcuaaa     2040

uuacugaaag gacuaagaga cucagaugaa uguucgauaa uagaacauau guggaauacg     2100

caacaaaauu ucaugcaagu acaagcagaa gaagauuucg cccaaagaau acaagaagaa     2160

aauaaccaac accuaaaagg aaacacacuc gaagaaguuu uagcagaugc uuauacauca     2220

ccucaaaaua agaaagcaau caggcaaguu gugaaaguaa ucgaagacau acaaaaagcc     2280

augggcggaa uagcacccca gaaaauaucg aucgaguuca cacgagcacc agaagagaaa     2340

cccagaagaa guguugagcg acaacaacaa auagagaaaa uauauaaaag uguagcuaau     2400

gaagucgaaa augcaggcgu uaaagaugaa uuacaacgcu uuucggacag aaaacaaacg     2460

cuaacagaca aauacuucuu guacuucacc caacuaggaa aagauaugua cacgggaaaa     2520

agcauaaaua uagaucaaau uggaaauuac caaauugacc acauucugcc acaagcauuc     2580

auaaaagaug acucacuuga caauagaguc cuaguaucug ccgcaguaaa caacgguaaa     2640

ucagauaacg ucccuaccaa aaacgaccga uauaaaguaa gagaacacgg acaaagauua     2700

uaugaccaag gccuuauaag caaacguaaa uucaacaauu uaauuacaga cccagacuca     2760

auaaauaaau ucacagcuag aggauucguc aaacgacaac ugguagaaac aucacaagua     2820

auuagacucg uagcagaguu ccuuuauaau aaauaucaga guaaauccga aauaauugaa     2880

guaagagcca aaaugaacuc acagcuacgg aaagauuucg gacucaucaa aaauagaaau     2940

guaaaugacu accaucacgc acuagacgca uacuuaacua cauuuauagg caacuaucua     3000

uacagaagau acccaaaacu acgagcauau uucaccuacg guugcuucaa gaaaguagac     3060

gauagucaac uaaaauacuu caacuuccua cacgacaucg aagacgacaa aaagaaaauu     3120

auaaaagaua augacacagg cgaaauacua uggcauagag acgaaggcau ugcaagauuu     3180

aaacgaauuu acaauuucaa auacaugcuc guaacacacg aaguacucac ccgacaggga     3240

gcaauguuca aacaaacugu auacuccgca gccguugcgg gaucuaagaa acuaauaccu     3300

guaaaagaag aucgcccaau agacauauac ggaggauaca caaauaauaa cgaugcuuuu     3360

cuagcuaucg ucaaaguaac caaaaagaaa caauacaugg uaguaggcau uccucaaaga     3420

gcaguaguaa aacucaacga cgccgccaaa caagguagca aggcuuacca aaaaguucuc     3480

uacaauguaa ucaaaccaca auucaucaaa aagaagaaag auagaaaaac agguaaaaua     3540

acacaaaaac ucgaaaauuu ugagauagua guaaguaaaa cccuuuaucg acaauuaauu     3600

auugacggug aucaaaaaua cauguuagga ucaucugccu acaaauauaa ugcgaaacag     3660

cuaguacuuu cugaauacgc ucuggaagug uuagacagau caauauucga gaagaaagaa     3720

uacacuacag uagacuuaga caauguauau gacgaaaucc uggcacaagu cgacaaauac     3780

uuuaguuuau augauaaaaa caaauucaga caaaaacuac augaaagccg caaaauuuuc     3840

acagaacugc ccauaaauaa ucucuucucu ggaacaaaac uccuuaaauc aggcaaaagg     3900

gaaacuuuaa augagauuuu ggaaggacuu cacgccaaug ccacuaagaa agaccuaaaa     3960

ucccuaaagu ucuccacacc acuaggaaug auucaauacc caucggggau aauccucagu     4020

gaaaacgcaa uacucuguua ucaaucacca acagggauuu ucgaaaggag aguaacacua     4080

aaagauuuau ga                                                         4092


<210>  5398
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5398
augacaaaac uuggaaaacc auaugguauc ggaguugaca uaggaagcaa cagcauugga       60

uuugcggcag uagaugaaaa cagccaucua auuaggcuua aaggaaaaac agugaucggc      120

gcuagacuau ucgaagaggg gaaagcagcu gcggaccgac gcgcuagucg cacaacccga      180

agaagacucu caagaaacag auggagauua aguuuucuac gagacuucuu cgaaagccac      240

auaaccccca cagacccuaa uuucuuuaug cgccaaaaau acagcgagau uaguccaaaa      300

gacaaaagcc gguauaaaua ugagaaaagg uuauuuaaug aucgaacaga ugcggaauuu      360

uacaaauaug aaaacugucc aacaauguau caccuucgaa accgacuaau gacagaucca      420

aguaaagccg auguacguga aaucuacuuc gccauccauc acauacuaaa aucaagaggg      480

cacuuuuuaa cacccggcga ugccaaagac uucaacacaa auaaaguagc cguuaauaaa      540

auauuccccg cauugcaaga ugcauaugca caaguauauc cagaccuaga uauaacauuc      600

gacgaaaaua aaguuaacga auuuaaaacc guacuucuca acgaaaaagc cacgcccucu      660

gauacacaaa gagcuuuagu gaaccuuuua uuagcuaaag aaggagaucg agacauucua      720

aaacaacaaa aacaagcacu cacggaguuc gcaaaagcag uuguaggauu aaaaacaaaa      780

cucaacguug cacuaggaac agaagucgau uccagcgaag caacugcaug gaauuucucc      840

cuaggacaac uagaugauaa auggaccgga auugaaucug caaugacaga ugaaggaaca      900

gaaauucuug aucaaauacg cgaucucuau cgagcucgac uacuaaaugg aauagugcca      960

gcugguaaaa cguuaucaca agcaaaaguc gaugacuacg cgcaacauca cgaagaccuc     1020

gagcuauuua aagauuaccu uaaacaguua gaggaugaug gaaccgcuaa agcuauccgg     1080

caguuauaug acagauacau cgacggcgau gacgcaaaac ccuucguuag agaagacuuu     1140

gucaaagcau uaaccaaaga aguaacugcg cacccaaacg caaaaagucc agaacuauug     1200

gaaagacucg cacaaccaga uuuuaugcua aaacaaagaa auaaggcaaa cggugcaaua     1260

ccaguacaaa ugcaacaaag agaauuagac caaauaauca aaaaccaaag cguuuauuac     1320

gacugguuag ccgcgccaaa uccaguagaa aaacaucgaa aaaguaugcc guaccaacuu     1380

gaugaauuac ugaauuuccg aauaccauac uauguuggac cucuaguaac agccaaagaa     1440

cagaaagcug cccaaggagg gguauuugcu uggaugguca gaaaagaucc agacgguaac     1500

auaacacccu acaauuucga cgagaaagug gacagagaag ccagcgccaa uacauuuaua     1560

cagagaauga aaacuacaga cacuuaccua auuggcgaag augugcuacc aaaacaaucc     1620

cuccuauauc aacgauauga aguccuaaau gaacucaaua acguaagagu caauaacgaa     1680

aaacuuagca uagaacaaaa acaacaagua auaagagaac ucuucgaaag acacaacagc     1740

guaacgauaa aacaauuugc ugaaaaucuc cgagcucaug gugacuacgc acacaugccc     1800

gaaauacgag gccucgcaga cgagaaaaga uuuuuaagcu cacuaucgac auaccgacaa     1860

uuaaaaaguu uacuaccaga agcaauagac gauccagcaa agcaagcaga cauagaaaau     1920

auaauagcau ggucaaccgu cuuugaagau gcggcuauuu ucaaaacaaa acugaaagaa     1980

aucaacuggu uagacuccca aacaaucaca aaacucucua acaucagaua cagaggaugg     2040

ggucaauuuu cacacaaauu ucuuaaugga cugacacucg gaaacggaca cacgauaaua     2100

caagaacuau ucuuaagcaa uaacaaccua augcaaauac uuacagacga aacacuacaa     2160

aagaaaauga cagaacuaaa ugcagauaaa cuaaaagccg caaauauaaa ugacgcuauc     2220

gaugauguuu auacaucacc uagcaauaaa aaggcacuuc gccaaguuuu aagaguaauu     2280

gaggacauua aaagggcagc ugacggacaa gaucccucau ggcuauaugu agaaacugca     2340

gacggcggag gaacacccgg aaaaagaaca agagcaagac agcaccaacu ccaagagaua     2400

uaugcaaaug cagcccacga auuaaucgau agugccguuc gaggugaauu agaagauaaa     2460

auagcagaua aagcagauuu caacgaccgg cucguauuau auuucaugca agggggccgc     2520

gacauauaca cagguacgcc cuuaaacaua gaucaacuau caaguuauga uauagaucau     2580

auucuuccuc aaucacucau aaaagacaau ucccuagaua accgaguguu aguacacgcg     2640

auaauaaacc gugagaaaaa ugcaacauuc gccucaacca uauaugccca gaaaaugaau     2700

gccacuuggc gccaauggca cgaagcuggu uuaauaagcg gcagaaaacu gagaaaccua     2760

caaaugcgac cugaccagau ugacaaauau gcaaguggcu ucguagcacg ccaguuagua     2820

gaaacaagac aaaucauuaa acuaacugaa caaauaguag ccgcucaaua cccugaaaca     2880

aaaauaaucg cagucaaagc cgggcuaagc caucaguuac gagaagaacu agaguucccc     2940

aaaaacagag acguuaauca cuaucaucac gccuuugaug cauuucuugc agccagaaua     3000

ggaacuuauc uauuaaaacg cuucccaaac uuacaagcau ucuucacaua uggcaaauuu     3060

aagaaagccg acguuaagaa acuaagagga uuuaacuuca uccgugacau cacucacgcu     3120

gaagauaaaa uaguaguaaa agacaccggu gaaguuauau gggacaagaa aagagaugug     3180

gaugaauuag acagaauaua uaauuucaaa cgaauguuga uaacucacga agugcacuuu     3240

gaaaaagcag accuauuuaa acaaacgguc uauggcgcua aagauuccaa agaagcuggg     3300

ggaaagaaac agcucauuuc aaagaagaaa gguuacccag uagauaucua cggggguuau     3360

acacaagaaa caggaagcua ucuaaguguc guuagacuca cuaagaaagc cauguaugca     3420

guuguaaaag ugucaaccag agaugcugca aaacuagcag uggcgaaauc uauaagcgaa     3480

caaaaagaaa acgaaacucu aaacgacauc auagacgaaa aauuauccaa aauaaguaaa     3540

aaugggaaaa uuacacacga auuauuugaa guaguuuuac cacgaguagg acagaaaaca     3600

cuauuuaaaa acucuaaaua caaucuuuuu cuagucaacu cggacacaua caugcauaau     3660

uaucaagaac uauggaugcc aagagaauau cagaaaaugu ggaaagauau cagccuaagu     3720

aaauacggug acgcgcaaac ugaagaccaa uuagaucaaa uuuucggcuu caucguuagc     3780

caagucaacu ccuauuucaa ucuguacgau aucaaucaau uccggaagaa auuaaaugau     3840

gccgcagaca aauuugcaac auuaccuaug cgagacacag acgacaugca aggaaaaauu     3900

gcuacuaucg gccaacuacu uauaggucuc caagcuaaug cagcacgcuc cgaucugagg     3960

aacuuagaca uaaaaacacc acugggccuc cuccaaaucc ccaauggcau aucuuuagac     4020

ucagacacaa guguaguuua ccaaagccca acuggacuuu uggaaagaga aguacaucug     4080

uccgaucuuu ga                                                         4092


<210>  5399
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5399
augaccaaac uaaaccaacc cuaugguaua ggccuagaca uaggauccaa uucuauaggg       60

uucgcuguag uggacgcgaa cagccaccua cuaaggcuaa aaggagaaac ggcuauugga      120

gccagauuau uuagggaagg gcaauccgca gcagaucgac gugaaucaag aaccacaaga      180

cgaagauuau caagaacaag auggagauua aguuuccuca gagauuucuu ugccccucac      240

aucacgaaaa uugacccaga cuucuuucuc cgacaaaaau auucagaaau uuccccgaaa      300

gacaaagaua gguuuaaaua cgagaaaagg uuauucaaug auagaacaga ugcagaguuc      360

uacgaagccu acccaucaau guaccaccua agacuccgac uaaugacaga cgcaucucaa      420

gcagaucccc gggaaaucuu cuuagcaaua caccacauac uaaaaaguag agggcacuuu      480

cuuacuccug gugcagcaaa agauuucaac accgaugaag uggauuuaaa acauaucuuu      540

ccugcacuag cagaagccua ugcgcaaguu uauccagauu uagaauugaa cuuugauuca      600

gcgcuagcag augauuuuaa agcaaaacua uuagaugaag augcaaaacc uucugacaua      660

caaaaagccc uaguuaacuu acuacuaucu ucggacggcg aaaaagaaau aguaaagaaa      720

agaaagcagg uauuaacaga auuugcaaaa gcaaucacug gcuuaaaaac aaaauucaau      780

uuagcauugg ggacagaagu agacgaagcu gacgccucaa auuggcaauu cuccaugggc      840

caauuggaug acaaauggag uaauauagaa acgucuauga cagaccaagg gacggaaauc      900

uuugaacaaa uacaagaacu auauagagcu agacuguuaa augggauagu acccgcagga      960

augucacuuu cgcaagcuaa aguagccgau uacggucaac acaaagaaga ccuagaacua     1020

uucaaaacau auauaaagaa acucaacgac cacgaacuag ccaaaacaau aagagggcug     1080

uaugauaggu auauuaaugg cgaugaugcg aaaccauucu uacgagaaga cuucguaaaa     1140

gcucuaacca aagaagugac ggcgcauccc aaugaaguau cugaacaguu acuaaauaga     1200

augggacagg caaauuucau guuaaaacaa aggaccaaag caaacggggc uauaccggua     1260

caacuacaac aaagagaacu agaucaaaua auagcuaauc aaucgaaaua cuaugacugg     1320

cuugcuaaac caaauccggu ggagaaacac agagcaagca aaccauacca auuagacgaa     1380

cuuuugacau uucguauccc cuacuauguc ggcccacuca uaacgccuaa acaacaggca     1440

gaaucaggag agaacguauu cgcauggaug guucggaaaa acccaucagg caauauaacg     1500

cccuauaacu uugacgagaa aguagacaga gaagcaucag caaauacuuu uauucaaaga     1560

augaaaacaa cagauacuua ccuaauagau gaagauguac uaccaaaaca aucucuacuu     1620

uaucaaaaau acgaaguauu aaaugaacuc aacaauguac guauaaauaa ugaagcccua     1680

agcacugauc aaaaacaaag acuaauaaga gaaguauucg agcggcauag caguguuacu     1740

auaaagcaag uagcagauaa uuuaguagca cacggagauu ucgcacaaag gccugaaauu     1800

agaggauuag cugacgagaa aagauuuuug uccucacuau ccacauauca ucaauuaaaa     1860

gaaauuuuac auggcgcaau cgacgacccc acaaaauuac acgauauaga aaacauaauu     1920

accuggucaa ccguauuuga agaugcugcg aucuuuaaaa caaaacuagc agaaauagau     1980

uggcuagacc cagcuacgau caagaaguua ucaaauauua gguacagagg uuggggacag     2040

uuuucccgaa aauuacuaga uggucuaaaa cuagggaaug gacauacagu gauacaagaa     2100

cuuaugcuaa gcaaccauaa cuuaaugcaa auacuugcgg augaaacauu aaaagaaaca     2160

augacugaau uaaaccagga uaaacuaaaa acagacgaca ucgaggaugu aauaaacgac     2220

gcuuauaccu ccccaucaaa caagaaagca cuccgacaag uacuucgugu aguagaagau     2280

aucaaacacg ccgcgaaugg acaagauccu aguuggcugu auaucgaaac agccgacgga     2340

ccuggaacag ccggcaaaag aacgcaauca agacaaaaac aaauacagac agucuacgcu     2400

aacgcugcac aagaauuaau agauucagca gucaggggcg aacuugaaga uaaaauugcu     2460

gauaaagcau cauucacgga ccgauuaguu cuauauuuca ugcaaggugg uagagauauu     2520

uauacugggg caccuuuaaa uauagaccaa cucucgcacu augauaucga ucauauacua     2580

ccacaaaguu uaauuaaaga cgauucacua gauaauagag uccuuguaaa ugcgacaaua     2640

aauagagaga aaaauaacgu uuucgcuucg accuuauuug ccggaaaaau gaaagccacc     2700

uggagaaaau ggcacgaagc aggccuaauu uccggaagaa agcuacgaaa cuuaauguug     2760

cgcccugaug aaaucgacaa auuugcaaaa gguuucguug caaggcagcu aguugaaacg     2820

cgacaaauaa uaaaacugac agaacaaaua gcugccgccc aauacccaaa cacaaaaaua     2880

aucgcaguua aagcaggccu aucucaccaa cuaagagaag aacucgacuu uccuaaaaau     2940

agagacguga accacuauca ccacgcauuc gaugcuuucu uggcggccag aauuggaacu     3000

uaccuauuaa aacgauaccc aaaauugaca ccuuucuuua cauaugguga auuugcgaaa     3060

guagacguaa agaaauuuag agaguuuaau uucauaggug cccucacaca cgcaaagaaa     3120

aauauaaucg caaaagauac cggugaaaua guaugggaca aagaacgaga uauacaagaa     3180

cuugauagaa uauacaacuu caaacgcaug uuaauaaccc augaaguuca uuucgacaug     3240

gcugauuuau ucgaucaaac aauuuacgca gcaaaagacu caaaagaacg cgggggaagu     3300

aaaaaucuua uacaaaagaa acaaggcuac uugacaggaa uauacggggg auauuccaca     3360

gaacgaggau cguacgugag cauuauuaga acauuuaaga aaggagcucc aauauacaau     3420

aucguaaaca uuaguaccgc ugacgucucc uuacuuucag auaaacagcg aguuagcgaa     3480

gcaucagcag acguccuucu aaaagaacuu auaaagaaga aacucgcaaa acguaaaagc     3540

ggcgaaaaua caaaaucagg ggcuuucgaa auaguauuac ccagaguugg caaaaacacu     3600

uuauucaaaa auaagaaaua uggacuauuu cuagucgaau cuacaacuga auuucauaac     3660

uaccaagaau uaugguugcc cagaaaugau caaauguuaa uaaaaucaau acaaucacau     3720

aaaauagaag aaucuaaagc agauuaugaa cuaacacgaa uguaucgcaa cauagcuuca     3780

caaguugacc auuacuuugg acuuuauaca auucguaaau ucucugaaaa auugacagca     3840

ucaauuaaug auuauggcgc uuuaccggua uauaaaucag aaaccacgga ugguaaauac     3900

gaaguacuau cuaacuuauu cgagggacua ggaaguggaa caucaacagg aaaccuuaca     3960

gcaguuaaac ugcaaccuaa uuuuggcagg guguauuuaa caaguggcau uguccuagaa     4020

gacgauacau cgauaauaua ccaaagucca accggucuau ucgaaagaag uguuaaauua     4080

gcagauuuau ga                                                         4092


<210>  5400
<211>  4092
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5400
augacaaaac uuaaucaacc guacgguaua ggcuuggaua uuggaagcaa uucaaucgga       60

uuugcaguug uagaugccaa cucacaucua cuucgacuaa aaggugagac ugccauagga      120

gcaagacuau uccgcgaagg ucaaucagcc gccgaucgac gaggaucaag aaccaccaga      180

cgaagauugu cacggacaag auggagacuc agcuuucuac gagacuucuu ugccccacac      240

auaacaaaaa uugaucccga cuuuuuccua aggcaaaaau auagcgaaau aucccccaaa      300

gauaaagauc gcuucaaaua ugaaaagaga cuauucaaug accgaacaga ugcagaguuc      360

uacgaagacu aucccucaau guaucaucuu agacuacacc ucaugaccca cacacauaaa      420

gcagauccua gagaaauauu uuuagcuaua caccacauuu uaaaaaguag aggccauuuu      480

cuaacgccag gagcggcaaa agacuuuaac acagacaaag uggauuuaga agauauuuuu      540

ccagcauuaa cugaagcaua ugcacaaguu uauccagacc uggaacuuac auuugaucua      600

gcuaaagcag acgauuuuaa agcaaaauua uuagaugaac aagcaacccc aucugauaca      660

caaaaagcuc uaguuaaccu acuacuuuca uccgacggcg aaaaagaaau aguuaagaaa      720

cguaaacaag uucuaacaga guucgcaaag gcaauuaccg gcuugaaaac aaaauuuaau      780

cuagcauuag gaacagaagu agacgaagcu gaugcaucaa auuggcaauu uaguauggga      840

caacuggaug acaagugguc caacauagaa acaaguauga ccgaucaagg cacagaaaua      900

uucgaacaaa uacaggaacu auacagagcu cgccuacuaa augguaucgu uccggccggc      960

augucacuua gccaagcuaa aguagccgau uaugggcaac acaaagaaga ccuagaauua     1020

uuuaaaacuu acuuaaagaa acucaacgac caugaacuag caaaaacaau aagggggcua     1080

uacgauaggu auauaaacgg ggaugacgca aagccauuuc uccgagaaga uuuuguaaag     1140

gccuuaacua aagaagucac agcucaccca aacgaaguau ccgaacaauu acuuaauagg     1200

augggccaag caaacuucau gcucaagcaa cgcacgaaag caaacggagc cauacccaua     1260

caauuacagc aaagagaguu agaucaaaua auagcuaauc aaucaaaaua uuacgauugg     1320

cucgcggccc cuaaucccgu ugaagcacau cgauggaaga ugccauacca auuagaugag     1380

uuauugaacu uccauauacc cuacuacgug ggaccacuaa uuacaccaaa acaacaagcc     1440

gaaagcggug aaaauguuuu ugcauggaug guucguaaag acccauccgg uaacauaacc     1500

ccauauaauu ucgacgaaaa aguagacaga gaagcaucag ccaacacauu cauucaaaga     1560

augaaaacca cagacaccua cuuaauuggu gaggauguac uuccuaaaca aucauuacua     1620

uaucaaaaau augaagugcu caacgaacuc aacaauguua ggauaaacaa ugaaugucua     1680

gggacagacc aaaaacaacg ccuaauacgu gaaguuuuug aaagacacag uucaguaacc     1740

auaaagcaag uagcagacaa cuuaguagca cacggcgauu uugcacgcag accagaaaua     1800

cguggauuag cugaugaaaa acguuucuua aguucacuca guacuuacca ccaacuuaaa     1860

gagauacuuc acgaagcgau agaugauccu acaaaauuac ucgacauaga aaauauaauc     1920

acauggagua ccguuuuuga agaucauaca auauuugaaa caaaauuggc agaaauugaa     1980

ugguuagacc ccaagaaaau uaacgagcug ucuggcauac guuauagggg uuggggacaa     2040

uucaguagaa aacuguuaga uggcuuaaaa uuaggaaaug gacacaccgu uauacaagaa     2100

uuaaugcuaa gcaaccacaa cuuaaugcaa auccuugcug augaaacgcu aaaagaaaca     2160

augacagaau ugaaccaaga caagcuaaag accgacgaca ucgaagacgu gauaaaugau     2220

gcauauacaa gccccucuaa uaagaaagcu cuaaggcaag uacuuagagu aguagaagau     2280

auuaaacacg cugcaaacgg ccaagaucca ucuuggcuau ucaucgaaac ugccgaugga     2340

acaggaaccg ccgguaaacg uacccaaucc agacaaaaac aaauucaaac aguguacgcc     2400

aaugcagcgc aagaacuaau cgauagcgca guccguggag aguuagaaga caaaauagca     2460

gauaaagcca guuucacaga uagauuaguc cuauacuuua ugcaaggagg aagagauauc     2520

uauacaggag caccccuuaa cauagaccaa cuaucccauu acgauaucga ccacauauua     2580

ccacaaucau uaauaaaaga ugacucgcua gacaaucgag uccucguaaa ugcaacaaua     2640

aacagagaga aaaacaacgu auuugcguca acuuuauuug cagggaaaau gaaggccacu     2700

uggaggaaau ggcacgaagc gggccuaauu ucagggagga aauuacguaa cuuaaugcua     2760

cgaccggacg aaauugacaa guuugcaaaa gguuuuguag caagacagcu aguagaaaca     2820

agacaaauca uuaaacucac agaacaaaua gccgcagcac aauauccaaa cacaaagauu     2880

auagccguua aagcaggucu aucacaccaa cuaagagagg aacuagacuu ucccaagaac     2940

agagacguaa aucacuauca ccaugcauuc gaugcauuuu uagcagcacg aauaggaaca     3000

uaucuucuaa aacgauaucc aaaauuagca ccguucuuua cauaugguga auuugcaaag     3060

guagauguaa agaaauucag agaauuuaac uucaucggag cacuaacaca ugcgaagaaa     3120

aacauaauag ccaaagacac aggagaaaua gucugggaua aagaacgaga uauaagagag     3180

cucgaccgca uauauaauuu caaaagaaug cuuauuacac acgaagucua uuuugaaacc     3240

gcagacuuau ucaaacaaac uauauacgcc gcuaaagaua guaaagaaag aggaggaucc     3300

aaacaauuaa uuccaaagaa acaaggauac ccaacacaag uguauggcgg auauacccaa     3360

gaaaguggga guuacaaugc ucuaguaaga guagcagaag cagacacaac ugcauaccaa     3420

guaaucaaaa uuuccgcuca aaaugcaucc aaaaucgcuu cagcaaaucu uaaaucucgu     3480

gaaaaaggga aacaacuauu aaaugaaauu guaguaaaac agcucgccaa aaggaggaaa     3540

aacuggaaac ccagcgccaa caguuuuaaa auagucauac cacgauucgg aaugggaacg     3600

cuuuuucaaa augcaaaaua uggcuuauuc augguaaaua gcgauacuua cuaccgaaau     3660

uaccaagaau uaugguuauc aagggaaaau caaaaacucc uuaagaagcu auuuucaaua     3720

aaauacgaga aaacccaaau gaaccacgac gcauuacaag uuuauaaagc uauaauagac     3780

caaguagaaa aauuuuucaa acuauaugac auaaaccaau uuagagcuaa acucucugac     3840

gcaauagaac gauuugagaa acuacccaua aauacugacg gaaauaaaau uggaaagacc     3900

gaaacauuac gacaaauuuu aauaggauua caagccaacg guacuagaag uaacguaaag     3960

aaucuaggua uaaaaacaga ccuuggauua uuacaaguag gcucaggcau aaaauuggau     4020

aaggacacac aaaucguuua ucaaagccca ucagggcugu ucaaaagaag aauaccucug     4080

gcagaccuau ga                                                         4092


<210>  5401
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5401
augggugacc gaaaauacaa ccuaggccug gauaucggga caucgucgau aggauuugca       60

gcgguugacg agaauaauca accgauuaga guaaaaggca aaacagccau aggaguucgu      120

cuguucgaag aaggcaaaac ugcagccgau agaagagggu ucagaacaac aaggagaaga      180

cuuucacgcc gucgcuggcg aaucaacuua uuaaaugaaa uauucgacgc acacuuagca      240

gaaguagauc caacauucuu agcaagacua aaagagagua aucgaaguaa ccuagaucca      300

aagaaaucau uccaaggauc acuucuauuu ccugaacgaa aagauuauca auuuuacgaa      360

gaauacccaa cuauuuauca ccuaagaaaa gcucuaaugg aaaaagaccg uaaauuugac      420

uucagagaga uauacuuagc aguccaucau auaauaaaau acagagguaa cuucuuaaac      480

ggaacaccaa ugcgaucauu uaaaguagaa aauauagaau uagacacccu cuuugaccaa      540

cuaaaucagc uauacgcaga aauuguuccc gauaacgaac ucgcauucga ucuggcucaa      600

guagcagacg uaaaagacgu uuugucaucu acaacgauuu auaaaaugga caaaaagaaa      660

caacugguaa aaaugaugcu ccuuccagca ucgaacaaag ccuuacaaag cgaaaacaag      720

aaaaucguaa cacaauuugu caaugcaaua cugaauuaua aauuuaaauu agaugucuua      780

uuacaaguag aaacagaugc cgacuggagu cuuaaacuua acgacgaagg agccgacgau      840

aaacuugaag aguucacggg agaccuugac gaaaauagac uugaaauuau agacuuacuc      900

caaagacucc auaauugguu cucacucaac gaaauaacga aagauggaaa uagcuuaucc      960

gcugcaaugg uugaaaaaua cgagaaucac caucaccacc ucggacucuu aaagaaaguc     1020

auagagaauc acccagacgc caagaaagca aaagcacuaa aagaaacaua cacagcauau     1080

gugggaaaaa ccgacgacaa aacccaaaac caagaugauu uuuacaaagc aguugaaaag     1140

aacuuagacg acucaccuga cgcaaaagaa auuaaacgcu uaauacaauu agaccaauuu     1200

augcccaaac aacguacggg ccaaaaugga gccaucccac aucaacuaca ccagcaagaa     1260

uuagaucaaa uuauagaaaa acagucuaaa uacuauccau ucuuagcgga acccaaucca     1320

aauguaaaaa gaagaaaaga cgcaccuuau aaacuagaug aauugauugc uuucaaaaua     1380

cccuauuacg uugguccgcu agugacgcca gaagaacaag cacagaacgg agaaaacguc     1440

uuugccugga ugaagagaaa agcggcugga ccaaucacuc cauggaauuu ugacgaaaaa     1500

gucgacagaa uggaaucugc aaaucgauuc auacguagaa ugacaacaaa agacacuuac     1560

cuauucggag aagauguacu cccagcagaa agcaugauau accaaaaauu cguaguauua     1620

aaugaacuca auaaccugaa aauuaauggg cgccaucugu cacuaaaaga caaacaagau     1680

gucuacaacg accuauucaa acaacaaaag acaguaucaa uaaaagcauu acaaaacuac     1740

uacguaacca aaaagaaggc cgcaacagcc ccaacaguag gcgguuuggc agacccaaag     1800

aaauuccuau caagccuaag uacauauaua gauuuuaaaa acauguucgg ugaaagagua     1860

aaugauccuc aguuccaaga agacuuagaa caaaucgucg aaugguccac aaucuuugaa     1920

gaucguggca uauuuaaagc aaaacuacaa gcacuaggau ggcuaucgga aaaacaaauc     1980

caacaauuag uggcuaaaag auauaaaggu uggggacggu uaucaaagaa auuacuuaca     2040

ggacucaaaa acgcagaagg auacucaauu uuagacgaaa uguggcgauc aacuggaaau     2100

uuuaugcaaa uccaaucgcg uccagaguuc gcagcacuaa uacaacaagc gaaugagaaa     2160

caauucgaag ggaaugaccc cgacaacguc ugggagaaua uugaaaauau acuaggagau     2220

gcauauacaa guccacaaaa uaagaaagcg auaaggcaag uagucaaagu uguacaagac     2280

auagaaaaag cgguagguaa uccacccgaa aagaucgcga uagaauuuac cagagaagca     2340

gcagcuaacc cccaaagaac gcaaucaagg cuaagaaccu uagaaaaauu auacgaauca     2400

gccgaagaag ugguagaugc aggguuaaca gcagaacucg cggaguucaa agaaaauaaa     2460

cacguacuuu ccgacaaaua cuacuuauau uuuacucaau uaggcagaga uguauacaca     2520

ggagacacaa ucagucuuga caaauuaaac gacuaugaug uagaucauau acuaccacaa     2580

ucuuuuauaa aagaugauuc acucgauaac agaguauuga cgaucagagc ugucaacaac     2640

ggcaaaagcg auaacguucc agcaaaaaug uuuggcaaga aaaugggcag cuucuggaga     2700

uaucucuuag auaacggaau gauaucaaaa agaaaauaua auaaucugau aaccgacccc     2760

gacaacauuu ccaaauacgc acaaaaagga uucauaaacc gccaguuagu cgaaaccuca     2820

caaguaauaa aacuaacagc aaacauacuu aauggaauau augauaaaga cacagaaaua     2880

auugaaguuc cagcaaaaau gaacucucaa augagaaaaa uguucgaccu aguuaaagua     2940

agagaaguca augauuauca ucaugcuuuc gaugcuuauu ugacaauuuu cauuggaaau     3000

uaucuauaca aaugcuaucc aaaacuucag ccuuauuucg ucuacgacaa uuuuaagaaa     3060

uucggaaaca aagaagauau uggccauaaa agauuuaauu ucuuaggaaa aauugaaaga     3120

gaaaagaaag uaguagcccc agaaacaggc gaaaucuugu ggucaaacgu agcaccaaac     3180

gaaacaauaa aacaaaucaa gaaaguguau gacuacaaau ucaugauagu cucgagagag     3240

auaacaacaa gacgagcgga acuauucaac caaacaguuu accccaaaaa uuaucauggc     3300

aaacuuauac caauaaaaga ggaucgaccu acugauuuau auggaggcua uucaggaaac     3360

acugaugcau aucuggcuau uguagcuuua gaagauaaaa agaaaggaaa auacuucaaa     3420

gucguaggua uaccgacucg aguagccgcc aaacuagaaa aauugaaaca gcaagacucc     3480

caacaauacc uacaagcacu ucauaaagug auugcuccac aauucacaaa aucaacaaag     3540

aaaggaauaa agaaaacaga guuugaaaua guacucgaua aaguacacua cagacaauug     3600

gugcaagacg guccaguuaa aaugaugcua ggcaguucua cauauaaaua caaugcaaaa     3660

caacucguuu uauccgaaaa agcacuccaa guuauagcug acgaucgaaa auuugacgaa     3720

acacagaaag acgacaaucu cauagcaguu uacgacgaga uacucaguau cguaaaccaa     3780

ucauucgauc uauaugacau aaacggauuu agaaagaaau uaaacgacaa ucgugaccaa     3840

uuuauagauc uuccggcaga aacuaaauac gaagggagaa aaguuguagc acacggaaaa     3900

agagaaauga uacuagaaau ucuaaaagga cuacacgcua augcagcauu cgguaauuua     3960

aaaccaauag gauucaguac cgccuuugga caacuacaag ucccaaaugg uauaaucuua     4020

ucgaaaaacg ccauauuaau acaccaaucu ccaaguggac uauucgagag aaaaaucaaa     4080

cuuucugauu uauga                                                      4095


<210>  5402
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5402
auggaaaaac aggaguauua ccuaggacua gacaugggaa uaagcagcgu aggaugggcg       60

gucacaaaug acaaauauga acuacuacgu aagaaaggaa aagacaugug ggguauaaga      120

gaguucgaag aagcggacuc aagcgcagaa agacgaacac acagaguaaa cagaagacga      180

agacaaagac aagucgcaag aaugggguua cuaaaagaau acuucucaga ugcaauagaa      240

aaaguagaua guaauuucuu uauacgaaua gaaaacucca aauauuucuu ugaagacaaa      300

gauggcagac uaucaucaaa gaaugguauc uuugaugaug cagacuauaa agacguagau      360

uauuauacag aauaucccac gauuuuucau cuuagaaaag cacuuuuaga caucgaggac      420

accggaaaag aauaugacgu ucgacuggua uaccuagcgc uaguaaacau guucaaacau      480

agaggucauu uccuauuaaa cacagaaggc gacgcacuaa guagcgagca aguaaauaca      540

accuaccaag aauuacuaga aauauuagaa aacaacuaug auauauccuu agaaucucga      600

gacauaaagg acguaauaga uauuauuuca agcucagaua acggaagaaa gaagaaacau      660

gaacaaauac uagaauuauu uggagugaag aaaucagaca aaagggcaaa ugaauuacua      720

aaaugucuau gugggcuuag cguaaaugca aaauuaauau ucgacgaaaa uuaugaagaa      780

aaaguagauu uguguuucca cgacuuuagc uauacagaca aaauuccaga cuugauggag      840

aaauuaagcg aggaagaaua uuacgucguu gaacaacuua aaagcauaua ugauuauuca      900

auauuagcag guacacuaaa agguuacgau uacuuaucau gcgcacgagu cgaacucuau      960

gaaaaacaca gagaagacuu aaaacuacuc aaagcacuuu acaggagaua caaaacgaau     1020

gaggaguaua acgacauguu uaaaagcgac aaagaaggaa cauacagugc auacguaaac     1080

ucauuuaaua guucagacuc uagugugaaa aucaaaaagu accgauggaa uaugaaagga     1140

agaacaauag auaacuuaua cgguacgaua aagaaggcau uuaaagauaa uacagaugac     1200

aaggauguac aaucaaugau ggaguccauu gacaaagaac aauuuuuacc aaagcaacua     1260

acaggagcaa auggggucau ucccaaccaa guacacaaaa aggaaaugga aagaauacuu     1320

aagaaagcgc aaaacuaucu accauucuua aaagaaaaag acgaaaguga auugaccgua     1380

agcgaaagaa uauugagacu uuuuacuuuu cagauaccau auuauguagg accauuaggc     1440

gaaaauucaa aaacaggaug gguuguacga cgggaaggcg guaggguacu acccuggaac     1500

auagaugaga aaauagauau gagcgcaacg agugaagcau uuauagggaa ccugaucaga     1560

aaauguaccu accuaucuga ugaaaaagua augccaaaag ccucacuaag uuaugaacgu     1620

uauuguguuu uaaaugaaau caauaauaua ugcauagaug gagaacgccu agauacggaa     1680

cuaaaacaga aaauauauac agaauguuuc augaaaggca aaagaguuac aagaaaacaa     1740

auaugcaacu aucuuguagc acaaggaugu auaaagagug augaacaagu uacaggaauc     1800

gauaucaacg ugaacucuuc ucugagcuca uacggcaaaa uguucgcaau cuuuggagaa     1860

cacuuaaaag aagacgcaau acggaagguc gccgaagaaa uaauauauug ggguacaaua     1920

uuuagugacu cuaagaaaau guuuaggaaa cacuuaacaa aauaugucga acaggguauu     1980

uugggagaau cacagguaaa acggauucua gguuacaaau ucaaggauug gggaagauuc     2040

ucaaaagcau uacuuacuuu acaaggaaua gauaaaucca cgggggaacu auuaucacuu     2100

gaaaacgcga ugugggaaua uaguuugaac uucauggaau uaauuaauuc ugaugauuuc     2160

acauucaaag acgagcuugc uaacagaaga acaucugcaa uuaagucauu auccgaauuu     2220

acauacgaag accuugagga uacauauuuu ucaguacccg uuaagcgaau gauauggcaa     2280

acuaucuuag uaguaagaga aauagaacaa auaauggggu acgcgccaaa gaaaguauuu     2340

guagaaaugg cgaggucaga agaagaaaaa ggugacaaag gcagaaaaga cagccgugga     2400

acucaauuac uagaacuaua caagaacauc aaaaauaccg aaaaccauga uuggaagaaa     2460

gagauaagcg aggcaaacga aucuggaaaa cuaagaucua agaaacuaua ucuauacuau     2520

acucagaugg gcagagauau guauacaggg gaagaaauaa acauagacau guuauacgau     2580

gacaguaugu acgacauaga ccacaucuau ccacgacauu auguaaaaga cgauagcauc     2640

gucaacaauu ugguauuagu uaauaggaga gccaaucaag acauaaagaa agacuuauau     2700

ccgauaccag auaaaaucau uagcaacccu aaaguacaag aguuauggaa aaugcuacau     2760

aaacaaaacc ucauuacaga cgagaaauau aaaagacuaa ugaguagauc uccauuuacc     2820

gaaaaacaac uaggagacuu cauugcacgu cagcucguag aaacuagaca gggaacaaaa     2880

gggauugcag aacuucuugg ucaauuguuc aaggacacag agauaguaua ugcaaaagcg     2940

accaauguau cagacuucag gagagauaac gguuuuguaa aaagcagauu aguaaaugau     3000

uuccaucacg cucaagaugc guaccuaaau aucguaguug gaaacguaua cuauaccaaa     3060

uucaccaaaa auccauggaa cuuuauaaag aaagauuuug acaaagaccg uaacaaaaac     3120

ggguauaacc ucgcaaaaau guucgauaga gauguaguaa gaggagacga cacggccugg     3180

aucgcaucua agaaagacac ccaaggaacu aucgaaacgg uaagaaaaau gaugcguaaa     3240

aauaccccau uaaugacaag aaugucuuuu gagcaacaug gaggaauugc uaacgcuaca     3300

cuguacucag caaagaaagc gaaaguagaa aauuacauac ccuuaaaaag cuccgauaua     3360

agacuaucag acguaaagaa auauggugga uuuacuuccg caacaguagc guacuacuuu     3420

auaguccacc auaaagaagg aaagaaagaa aauacaacaa uagaagcauu accauuauau     3480

uguaaacgaa aaguagaaaa cgaagaaaau ggacuacuaa aauacuguga agaaguguua     3540

ggauacggag acgucaaaau auuguucaac aaacuuaaaa uccaaucacu acuagauauc     3600

aacgguuacc aagcucauuu aucaggaaaa acuggcaacc aacuaacccu aaggaaugcc     3660

augaaccucu gcguaccaga aaaauggacg gcauauauaa agaaauuaga aaaauuaguc     3720

gugggacacg ugaaagaaca agagauaagu ucagaagcaa auauagaacu auacgauuug     3780

cugaccgaua aacacuuaaa uagcguguau gccaaaagac caaaugcaua cggacaaaaa     3840

cucauagaca acagaucaag auucguaaau uuaaaaccgg aaucucagug ugaagcaaua     3900

cuacaaauau uaaaacuuac aaaaauagga aauaaugaaa caaaucugaa acuaaucggg     3960

gaaucagagc acucaggaaa aauguuaaua agcaaaaauu uaaaagacac agacaaacca     4020

gcaaucauau gccaaucagu aacaggacug uaugaacgca aaauauacau auucgaugaa     4080

caacuagaca aauga                                                      4095


<210>  5403
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5403
augaacaaac gcuauaauau uggucuagac aucgggacua auagcauagg uuggucagua       60

gucaaugauc aaucacaacu uguuagaauu aaaggaaaac uaggcuaugg aguacgcuua      120

uacgacgagg ggcaaacagc agaagaccga cggcucuuua gaaccacaag acgaagacug      180

aagagaagaa aauggcgccu uggcuugcuc agagaaauau ucgaaccaua uauuacuccu      240

caagaugcca cauuuuuccu aagacaaaaa caaucaaauc uauccccuaa agaucuacaa      300

aaacaacaac cacaaauagg cuuauucaac gauagauuag auagcgacuu ccacuaucaa      360

uacccaacca uauaucaccu aagauaugca uugaugacug aaaaacgcca auucgaucua      420

cgugaaauau auuuagcaau gcaucacauu guuaaauaca gaggacauuu ccuuaaugca      480

gcagcggcau ccagcuuuaa aagcggagaa auagaucuag cuaaagauuu cgcagcacua      540

aaccaaauau uuggcacaau cuuuagugaa guggguuuua guuuauccga aucgaaucua      600

gacgaaauaa aaagcauauu auuagauaac aaccucucag caagcgugag acaaaggaaa      660

gcccuaccau ugauauacca aacggcggca agcaaagacu cugaaaaaca auacaaagca      720

auagccacug aauuacuaaa agcuauauug ggauuaaaag caaaauucaa ucuuauaaca      780

gguauugagg uagaagacgu aaaggcuugg acauuauccu ucaacgcaga ggauuuugau      840

gcaaaacuaa gcgaccugaa uauugccgau aacgacgaug caguacaaau cauagaaauc      900

cuccgcgcgu uauacucugg aguauuauug acagguauag ugccggaagg acaaaguaua      960

ucacaagcua ugauagacaa auacgaagca cauaaagcac aucuuauuau guuaagauca     1020

auucgugaac aauuaucgga uaaagaaaau aaagcacuag aaaaggcuua ugacgauuac     1080

auugaucacc ccgaagauaa acaacacggg agcagagaag auuuuuaugc ugcaaucaca     1140

aaaguacuaa agaaagauuc agaagauacu aauaucauag aaauacuaaa auuaauagaa     1200

cuggaccagu ucaugccuaa acaacguaca aaagauaaug gagcaauccc ccaucaacua     1260

caccaacaag aacuagacca aauuauagaa aaccaaaaac aguacuaccc auggcucgcu     1320

gauauaaauc caaacgaaca acgacagaaa auggcaccau auaagcuaga ugaacucguu     1380

acguucaggg uuccauauua cguaggucca augauaacag cagaagaaca auuucaaacu     1440

ucaaauaaga aauucgcaug gaugauacga aaagaaccag gagcgauaac accuuggaac     1500

uucgaccaaa aaguagauag acaagcaagc gcaaaugaau uuauuaaacg caugacuacg     1560

acugacacuu accuucucgc agaagauguc uuaccaaagc aaucacuuuu auaccaaaga     1620

uuugaaguac uaaacgaacu aaauggaauu aaaaucgacg accagcccau aaccguagaa     1680

caaaaacaag cuaucuuuaa ugauaaauuu augcaacaag cccauguaac cgucaaaaac     1740

auacaagcau accuaguauc cgagggccaa uaugcaagua gaccuaacau cacaggacuu     1800

agcgacggag aaaaauucaa uaguucgcua ucgacguauc acgacuuaaa gaaaauuuuc     1860

ggagauauga uagaugaccc caauaaacaa acagauuuug agaaaaugau agaauggucg     1920

acuauuuuug aagacggcaa aaucuuugcg gauaaacuca gggaaauuaa cugguuaaca     1980

gaugaacaaa gacaaagauu aucuaacaaa agauacagag gguggggcca acuaucaacc     2040

aaauuacuca cuaaaauugu agaucaaaac ggccaaagaa uaauagacau ccuauggaac     2100

acaaaugaca acuucaugcg cauaauacac ucagacgauu uugaccaagc gguuacccaa     2160

gcgaaccaaa uguugcuagc ugacaacgac acucaagaug ucauaaauga acuguauaca     2220

agcccaucaa acaagaaagc ucugaggcaa auacucuuag ugguugcaga uauucaaaaa     2280

gcaaugaaag gacaagcccc ugcaagaaua cugauugaau uugcacgaga aaaacaggca     2340

aacccuagac uaucaacuca acgaagacga cagguagaac aaguauacca agaauuaaac     2400

aacgaucagu uaaauaaaga auuagcagac ccacaauaug cuaaccuauc aaaagauaga     2460

cuauuccuau auuucaugca gggaggaaga gacauguaua ccggcgaaag ccuuaauaua     2520

gaucgacuca gcgaauacga cauugaccac aucuuaccac aagcguucau uaaagacaac     2580

ucacucgaua accguguacu cgucuccgcu aaaaugaacc guaguaaauc cgaucacgua     2640

ccacuagauu ucacugagga acguuucggu cagaaaaugc gaucacagug ggaacaaaug     2700

cuacgugccg gacuacuuac aaggcguaaa uacaauaacu ugaccuuaaa cccugauucc     2760

cuuaacaaau auacaauguc aggguucauu aauagacaau uaguggaaac cagacaagua     2820

aucaaacuag ccacaaaccu cuuaguagac caauacgccg augaagacgu gcagcuuguu     2880

acagucaaaa guggauuaac gcaccaaaug agagaagacu ucgacuuucc uaaaaacaga     2940

aaccucaaca auuaccauca ugcuuucgau gcauaucuca cagccuucgu aggauuauau     3000

uugcuaaaga aauacccaaa auuagcccau uacuuugucu auggccaaua ccaacaagga     3060

uuuaaagcca augaucaaug gagaaauuuc aacuuuuuaa auggauuaag acaagaacaa     3120

caaguugacg cagagacagg agaauugauc uggcaaaaag accacgaccu cgcauauaug     3180

aacaaaaucu augauuuuaa gaaaauauug guaacacgcg aagugcauac uaaccauggc     3240

gcccucuaua aucagacacu cuauaaagca aaagacgaca aagcaucagg ucaaggaggg     3300

cggcaauuaa uagcggccaa agaaaauaga ccuaccgcca uuuauggugg auauuccgga     3360

aaaacaguag cauauaugug ccuaguacga aucacagaaa agaaagggga cuucuaucga     3420

auauguggag uacaaacagc auggguagac gaauuagaac auauagaaga cgcuaccauc     3480

aaacuccaaa gacuaacugc ccuacuaaca ccgcaauuua gcgcaacaaa gaaacaaaaa     3540

gacgaaguag ucacaguagu gaaaccauuu gaaguacuuc uaccuaaaau ucuaaucaau     3600

caaagauuuu acgacggagg acaagaacuu acacuagcau ccgcaaccua caaaaauaac     3660

gcccaagaau uaguacuaga uaagaaagcc guacaaguau ugaaugguca acucccaaaa     3720

gccgaacuag aagcucaagc aacaguugua uaugaucaga uucucgaacg agugcaaaaa     3780

uacuucccau uauacgauau gaauagcuuc cgagaaaaac uuuuuguagg uagagauaaa     3840

uucaaugagu uaccauggca agaccaaugg gaaaaccaua aacuaaucca aguaggucag     3900

aaaacaaucc uagaucgaau acuaauugga uugcacgcaa augcugccau gggggaccua     3960

aaagcauuaa acaugagcac acccuucggc aaacuacaaa aaucuaacgg aauaucauua     4020

ucuccagaaa cacaaauaau auaccaauca ccaacaggac uuuucgaaag aagaguugcu     4080

cuuaaagauc uauga                                                      4095


<210>  5404
<211>  4095
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5404
augaaaaacc uaaaagaaua cuauauagga cuagacauag gaacagcauc agucggaugg       60

gcaguaacug acgaaucaua uaacauacca aaauuuaaug gcaagaaaau guggggaguu      120

cgccuguuug acgaugcuaa aacagcagaa gaacgcagaa cacaaagagg aucaagacgg      180

cgauugaaca gaagaaaaga aagaauaaac uuauuacaag aucuuuucgc aaccgaaaua      240

uccaaaguug auccaaacuu cuuucuaaga cuagacaacu ccgaucuuua ucgagaagau      300

aaagacgaga aacuuaaaag uaaauacaca cuguucaacg acaaagacuu caaagaccga      360

gacuaccaca agaaauaccc uacaauccac caucuuauaa uggaucuuau cgaagacgaa      420

gggaagaaag acauaagauu acuguaucua gcaugucacu acuugcuuaa aaacagagga      480

cacuuuauau ucgaaggaca aaaauuugau accaaaaacu ccuuugauaa aucaauaaac      540

gaucuaaaaa uacaucuacg agaugaauau aacauagacc uagaauuuaa uaaugaagau      600

uuaaucgaaa uaauaacaga uacaacacua aacaaaacaa acaaaaagaa agaacucaaa      660

aacaucguug gagacaccaa auuccuaaaa gcaauaagcg cgauuaugau agguucaagu      720

caaaaacugg ucgaucuauu ugaagacggc gaauuugaag aaacaacggu caaaagcgug      780

gacuucucaa cuacagcauu cgaugacaaa uacagcgaau acgaagaagc acuuggugac      840

acgauaucau ugcuaaauau cuuaaaaagu auuuacgaca gcucaauuuu agaaaaucua      900

uuaaaagaug cagauaaauc aaaggacgga aacaaauaca uaucuaaagc auuuguaaag      960

aaauucaaca aacauggcaa agaccucaaa acccugaaac gaaucauaaa gaaauaucua     1020

ccgagcgaau acgcgaacau auuucgaaac aaaagcauua acgacaauua uguugcauac     1080

acaaaaagua auauaacauc aaacaaaaga acaaaagcgu caaaauuuac uaaacaagaa     1140

gacuucuaca aauuuaucaa gaaacaucuc gauacaauua aagaaaccaa acuuaacagc     1200

agcgaaaacg aagaccugaa auuaaucgac gaaaugcuca cagauauaga guucaaaaca     1260

uucauuccga aacucaaaag cuccgauaau ggcguaauac ccuaccagcu gaaacucaug     1320

gaacucaaga aaauucuuga caaucaaucu aaauauuacg acuuccuaaa cgaaagugau     1380

gaauauggua cagugaaaga uaaaguagaa aguauuaugg aguuccguau accguauuac     1440

gucgguccau uaaacccaga cagcaaauau gcauggauaa aaagagaaaa cacaaaaauu     1500

accccuugga auuucaaaga uaucguagac uuagacucaa gucgagaaga auuuauagac     1560

agguuaaucg gaagauguac auauuuaaaa gaagagaaag uucuaccaaa agcaucacua     1620

auauacaacg aguucauggu gcucaaugaa cuaaauaauc uuaaacucaa ugaguuccua     1680

auaacugaag aaaugaagaa agcaauuuuu gaagaauuau ucaaaaccaa aaagaaagua     1740

acauuaaaag caguaucaaa uuuacuuaag aaagaguuca auuuaacagg ggacauacua     1800

cuaucaggca ccgacggaga cuuuaaacaa gggcuaaauu ccuacauaga uuucaaaaac     1860

aucauagggg auaaaguaga cagggaugac uaccgcauaa aaauagagga gaucauaaaa     1920

cuaauagucu uauacgaaga cgauaaaaca uaucuuaaaa agaaaaucaa aucagcuuac     1980

aaaaacgauu ucacugauga ugaaaucaag aaaauagcag cacuaaauua caaagacugg     2040

ggacgucucu cuaaacgauu ucugacaggu aucgaaggcg ucgacaaaac aaccggagaa     2100

aaaggcucca ucaucuacuu caugcgcgaa uauaauuuaa acuuaaugga auuaaugucg     2160

ggucacuaca cauuuaccga agaaguagaa aaacucaacc caguagagaa uagagagcua     2220

ugcuacgaaa ugguugauga acuuuaccua ucaccauccg uaaaacggau guuauggcag     2280

ucccuaagag ugguugauga gauuaaaagg auaauaggua aagauccgaa gaaaauauuc     2340

auagaaaugg caagagcaaa agaagccaaa aauucucgca aagaaucacg uaaaaacaaa     2400

uuacuugagu ucuauaaauu cgguaagaaa gcauucauaa augaaauagg agaagaacga     2460

uacaacuacc uacucaacga aauaaacagc gaagaagagu cgaaauucag augggacaac     2520

cuuuacuuau acuacacaca acuuggccgu ugcauguaua gcuuagaacc aaucgaucua     2580

gcugauuuga aaaguaacaa cauauaugac caagaccaua uauacccaaa aaguaaaaua     2640

uacgaugacu cauuagaaaa cagaguccuu guaaagaaaa auuuaaauca ugagaaaggg     2700

aaccaauacc ccaucccaga aaaaguacua aacaaaaaug cauauggauu uuggaaaauc     2760

cuauucgaca aaggacuuau uggccagaag aaauauacuc gacuaacccg aagaacacca     2820

uuugaggaaa gagaguuagc cgaguucauu gaaagacaga uaguagaaac ccggcaagca     2880

acaaaagaaa cagcaaaccu acucaaaaau auaugccaag auucugagau agucuauagc     2940

aaagccgaaa acgcguccag auucagacaa gaguucgaca uuauaaaaug uagaacagua     3000

aaugacuuac accacaugca ugacgcauau cuaaacauag ucguugggaa cguauauaau     3060

acgaaguuca ccaaaaaccc guuaaauuuc auuaaagaua aagacaacgu acgcucguac     3120

aaucuagaaa auauguuuaa auacgacgua guaagagggu ccuacacagc auggaucgcg     3180

gacgauagcg aaggcaaugu uaaagcagca accaucaaga aagucaaaag agaauuagaa     3240

ggcaaaaauu auagauuuac aagaauguca uauaucggca caggaggucu auaugaccaa     3300

aauuuaauga gaaaaggcaa aggacaaauc ccucagaaag aaaacacaaa caagucaaac     3360

auagaaaaau auggagguua caauaaagca uccucagccu acuucgcauu aauugaaagc     3420

gauggaaaag cugguagaga aagaacacuc gaaacaauac caauuauggu uuacaaccaa     3480

gaaaaauacg gaaauacuga agcggucgac aaauaucuca aggacaaccu ggaacuacaa     3540

gaucccaaga uacuaaaaga caaaauuaaa auaaauucau uaauaaaacu ggacgguuuc     3600

uuauauaaca uaaaaggaaa aacaggcgau agccuuucca uagccgguuc aguacaacua     3660

aucguaaaua aagaagaaca aaaacuuauc aagaaaaugg auaaauuccu uguuaaaaag     3720

aaagacaaua aagauauaaa aguaacaucc uuugacaaca uuaaagaaga agaacugaua     3780

aaacuauaca aaacacuuag ugauaaauua aauaauggga uuuacucaaa caaaagaaac     3840

aaccaagcca aaaauauuuc ugaagcauua gacaaauuca aagaaauaag cauagaagag     3900

aaaauagacg uacucaauca aaucauacua cuuuuccaaa guuauaacaa uggcuguaau     3960

uuaaaaagca uaggacucuc agcaaaaacc ggaguaguau uuauacccaa gaaauuaaau     4020

uacaaagaau guaaacuaau uaaccaaucc aucacuggau uauuugaaaa cgaaguagac     4080

cuacuuaacu uauga                                                      4095


<210>  5405
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5405
auguacaaga aagauuauac cguaggacuu gauauaggca gcacaagcgu uggauucgcu       60

gcaauagacg acgacuacca accuaucaga uuaaaaggua aacgcguuau cggggucaga      120

cuauuugaag aaggaaaaac ggcagcagaa agacgaaauu uccgcacaac gcgucgaaga      180

uuaaaucgaa gaaaauggcg acuuaguuua cuugaagaaa uuuucgaccc auauauggcu      240

aaaguugauc cuacuuucuu cgcucgauua aaacaaucca aucucuuccc caaagacaaa      300

aacaagaaau ucaccggauc acuucuauuc ccaaaaacaa cugacagcaa auuuuaugau      360

gaauauccaa cuauuuauca ucuccgauau caucuaauga acgauagucg uaaauuugau      420

cuaagagaaa uauucuuagc aauucaucac auaguaaaau acagaggaaa uuuccucaau      480

uccgccccca ucgcuuccuu cucaacagau aaaauuaauc ucguagacga cuucgaaacc      540

gucaacgccg cauaccucca aauaaacagc uccaauccug uacaguuaga uacugaccaa      600

gcggaucaag uaagcagaau ccugcucgau aauaaacuaa gcaaccuuga caaacaaaaa      660

caagcagcua aagcacuagc acagaaaagu ggggacaaga aacaagacaa aauaaauaac      720

acaacaauaa aacaaauaug uaaagccuua uuagguuaca aauuugaccu agcuguagua      780

cuaaauauag acagugacga ccacaacaaa uggaaacugc aacucaguga ugaaaauauc      840

gacucgagac ucgccgaacu cacuggagaa cucaacgaag cccaauugga cauauuaaau      900

guccugagcc gauuauauag ccagauuaca cuaaaugaua ucguacccag ugggaugaca      960

cuauccgaag cuaugaucga aaaauauaaa gcccauaaga aacauuuaaa aaugcuaaaa     1020

gauuuugcca gaucacuuag uaagaaagac uauggucaag uaauggaggc uuaugccgaa     1080

uacauaggaa auauacccga aucagccucc ucuaaagcuc aaaagaaaau cagccaagac     1140

gacuucuaca agaaaauaaa aagauaucua gacgauucca aacugggaca gaaaauugca     1200

gaauuaaucg ccaaaaauga auuuaugcca aaacaacgaa cuaaucaaaa cggcguaaua     1260

ccuuaccaac uacacuuaaa agaacucaau caaauaauag aaaaccaauc aaaauacuau     1320

ccguggcuag cagaauuaaa ucccaacgag aaaaggagag gaaaagcaaa auauaaacuu     1380

uccgaacuug uagcauuuag aaucccuuau uauguaggac cacuaauuac acaagaagaa     1440

caacaaaaga ccaguaacgc aaacuuugca uggaugauac gaaaaagcga agguaggauc     1500

acaccaugga acuucgauga aaaagucgau cggauagcga cugccaaucg uuucauaaaa     1560

agaaugacua caaaggauac auaccuaauu ggugaagaug uccuaccagc acacucauua     1620

cuuuaugaac gauuuaaagu acuaaacgaa uuaaacauga uuagaguuaa uaaccaccga     1680

uuaucagucg gacaaaaaca agauguauuc gaaaaacucu uccaaaaaca caaaaccgua     1740

aagacucgag aacucauaaa acaacuaaug gcuucaggcu uaccaacgca accaagaaua     1800

acaggauuau cagaccccgc aaaauuuaac acaagccuag gaacauauaa uaacuucgcc     1860

aaaauacucg gagacgaaau aaaccaacca gaccgucaag aagacuuuga gaaaauaauc     1920

gaauggauaa caauauucga ugauaaagau auuuuaaaaa cuaaacucuc cgaauuauca     1980

uggcuggacg aaaaacaaau uaaugccuua auuaacaaaa acuauucagg auggggacgg     2040

uuaucacgaa aacuacuaac agaauuaacc aacaagaaag gacaaucgau aauagaccgc     2100

auguggaaca cacagaaaag uuuuauugaa aucaucaaug aacccgucuu ugcagaacaa     2160

auuaaagacg ccaaccaaga ccaacuugau gaagacgacu acgaaaccgu ucuacacgau     2220

gccuauacgu caccacaaaa caagaaagcc auaagacaag uuauuaaagu agucgaugac     2280

auagucagag ccgccggcag agcaccaaaa uucauuucac uagaauuugc acgcgaagac     2340

caggaaucga gaagaaccca aucuagacua ucucggauaa agaaaauuua cgaaacaacc     2400

accaaagagc uaguagccaa cgaucagaua agagaagaac uuggaaacgu caaagaucua     2460

aacgaucgau uguucuuaua uuuuacccaa cucggaagag acgcuuauac uggagaaagg     2520

auaaacaucg augauauauc cacaaaauac gauauagacc acauauuccc ccgcgcauuu     2580

aucaaagacg acucccucga uaauaaagug cuaguaaaaa gagcuguuaa caauggaaaa     2640

aguaauaaug uaccacucaa acucuucgga gccaaaauga aaccaauaug gaaaaugugg     2700

aaagaccauc aacuaauuuc uaaacgaaaa uaugaacacc uaaugacuaa ccccgacgca     2760

aucgauaaau acaaagccaa uggauucaua aauagacaac uaauugaaac acgucaaaua     2820

auaaaacuag ccgcaaauau acucgcaaac agauauucga cagaagacac gaaaaucaua     2880

gaagucaaag caaaucucaa ucaccaacuu cgacaaucuu uacaccuuua caaaaaccgc     2940

caaguaaaug acuaucauca cgcugucgau ggcuacuuau cagcauuugu cggccaauac     3000

cuauaccacc gauacccgag ucuacaaagc uacuucguau acggucaaua ccagcgauuc     3060

uucgauaaac aaguucaaga aaacaugaaa uuuaacagau ucaacuuucu auaugaccuc     3120

acagacagag auaacgauca aauaguuguc aaaaguacag gcgaaaucgu aggagaaaaa     3180

uccaaacuca uagaacaaau uaaaugggua uaucgcuaca aauucaugcu aguaagccaa     3240

gagguauuca ccaaaacugg accuuuguuc gaucaaacaa uauacccugc agggaguaau     3300

aaaucacgau uaauaccaau caagaaagac agacccacag acauauacgg gggauauagc     3360

ucgucuacag acgccuacau gagccucauc agggucccag auaagaaaga agacaaauac     3420

aaaguagucg gaguaccuuc ccgaagucuu gcaucucuaa aucgagcaga aaaacgcggg     3480

caugaugcau acuuagauca auugagacaa guaaucgcca aaucacaaaa gaaagaguuc     3540

caaauaauaa uacccaaacu cagauacaga caacuuauaa ucgacggagg auugaaauau     3600

accuuaggcu cauccgccua ucaauacaac gcacgucagc ucguccuauc agaaaaaucu     3660

auggaagucc uaguaacaga cuuuucaaaa guaacaaccg auuuagacaa acaaaaccaa     3720

aaacucaucg cuguauacga cgacauacua gacaaaguaa aucgauguuu accucuauac     3780

gacaaaaauc aauucagaaa agcccucaau ggugcccgug acaaauuugu acaguugccu     3840

auacuaccaa aauucaacgg aaacaagaaa gaagaagcag guaaaguuga aauacuaaac     3900

gaaauuuuau uaggacucca cgaugaugcu agcacaggaa aucucaaacc ucuaggaaua     3960

aaaacgcccu uuggaaugcu ccaaacacaa agcgguauaa cgaugucauc uaaugcugua     4020

auuguuaacc aauccccaac aggacucuuu gagcaccgua uauaucucaa agcgcuacua     4080

aacaaauuaa acaaauga                                                   4098


<210>  5406
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5406
augaccaaac uuaaucaacc uuauggcauu ggacuugaua uaggcucaaa uucaauagga       60

uucgcuguug uagacgccaa uagccauuua uugagauuaa aaggagaaac agcaaucgga      120

gcuagauuau uuagagaagg acaaagcgca gcugacagac ggggaucaag aacuaccagg      180

cgacgauuau ccagaacaag auggcgccuu agcuuccuua gagauuucuu ugcgccccau      240

auaaccaaaa uagacccaga cuucuuucua agacaaaaau acucugaaau cucaccuaaa      300

gacaaagacc gauuuaaaua cgagaaaaga cuauuuaacg auagaacaga cgcugaauuu      360

uaugaagacu auccaucuau guaccaucua cgacuacacc uuaugacaca uacccauaaa      420

gccgacccaa gagaaauauu ucuugcaauu caccauauuc ugaaaucuag aggucauuuc      480

uugacaccag gcgcugcaaa agauuuuaau accgacaaag uagauuuaga agacauauuu      540

ccagcuuuaa cagaggcuua cgcacaagua uauccagauc uagaauuaac auucgauuua      600

gcaaaagccg acgauuuuaa agccaaacuu uuagacgaac aggccacgcc cagcgauaca      660

caaaaagccc uugucaagcu auuacuaucc ucggacggag aaaaagaaau cguuaagaaa      720

agaaaacaag uccuuacaga auuugcaaaa gcaauaacug gacuaaaaac aaaauuuaau      780

uuagccuuag ggacagaagu cgacgaggca gacgcaucua acuggcaauu cucuaugggc      840

caacuugaug acaaaugguc aaacauugaa acaucaauga cagaccaagg uacagaaaua      900

uuugagcaaa uacaagaguu guacagagca agacuuuuaa acgguaucgu uccggcagga      960

augucacuuu cccaagcuaa gguugcagau uauggccaac auaaggaaga ucuggaacuu     1020

uuuaaaacau aucuuaagaa auuaaacgau caugagcuag ccaaaacuau uagaggcuua     1080

uacgaccgau acauuaaugg agaugacgcc aaacccuuuc uucgugaaga uuucguaaaa     1140

gcacuaacaa aagaagucac ugcacaccca aaugaaguau cggaacaauu acuaaacaga     1200

augggacaag caaauuuuau guuaaaacag agaacgaagg caaacggagc aauuccggua     1260

caacuucagc agcgagagcu cgaccaaauc auagcuaauc aaucaaaaua cuacgauugg     1320

uuagcaaaac caaaucccgu cgaaaaacac agagcaagua aaccauauca acuagacgaa     1380

uuacucacau ucagaauacc auacuauguu ggacccuuaa uaacgccaaa acaacaagcc     1440

gaaucaggag aaaacguguu cgcauggaug guuagaaaga aucccuccgg aaauauaacg     1500

cccuacaacu uugaugagaa aguagaccga gaagccagcg cuaacaccuu uauucaacgc     1560

augaaaacaa cugacacgua cuuaauagac gaagacguac uacccaaaca aucucuacuc     1620

uaucaaaaau acgaaguuuu aaacgaacuu aauaauguuc gcaucaacaa cgaagcccua     1680

agcacugacc aaaaacaacg acuaauacau gaaguuuuug aaagacauuc aucagucacu     1740

auuaaacaag uaguagauaa ccuaguagca cacggugauu uugcacaacg ucccgaaaua     1800

agaggacuug cugacgagaa aagguuccuc ucuagucuau cuacguacca ucaacugaaa     1860

gaaauauuac acggagcuau agaugauucc acaaaauugc acgauaucga aaauaucauu     1920

acauggucaa cagucuucga agacgcugcu auauucaaaa ccaaacuagc ugaaaucgau     1980

uggcuagacc cagcuaccau aaagaaacuc aguaacauuc gcuauagagg auggggacaa     2040

uuuaguagaa aauugcucga uggccuaaaa cuugggaaug gucauacagu aauacaagag     2100

uuaauguuaa gcaaucauaa uuuaaugcaa auauuagccg acgaaacccu aaaagaaacu     2160

augaccgagu uaaaccaaga caaauuaaaa acugaugaua uagaagacgu aauaaaugau     2220

gcauacacca gcccuagcaa uaagaaagca cuucgucagg uccuuagagu aguagaagac     2280

auaaaacaug ccgcgaacgg acaagaccca agcugguuau acaucgaaac ggcugauggu     2340

acaggcacag cagguaaaag gacucaaagu agacaaaaac aaauccaaac uguuuaugca     2400

aacgcagcac aagaacuaau agacagugcu guuagaggag aauuagaaga uaaaaucgca     2460

gacaaagcau cauucacaga cagauuaguu uuauacuuua ugcaaggcgg ccgagacaua     2520

uauacuggag cuccucuaaa uauagaccaa uugucucacu augacaucga ccauauacua     2580

ccccaaaguu uaauaaaaga ugacucacuc gauaauagag uucuaguaaa ugccacgauu     2640

aauagggaga aaaauaacgu auucgcgagc acucuauuug cuaguaaaau ggcagcaaca     2700

uggcguaaau ggcacgaagc aggauugaua ucaggacgaa aacuaagaaa ucuaauguua     2760

aggccagacg aaacagacaa auucgcucac ggauucgucg cucgccagcu aguagaaacu     2820

agacaaauua ucaaauuaac agaacaaauu gcagcggcac aauaccccaa cacuaaaaua     2880

auugcaguca aagcaggcuu aucacaccaa uuaagagaag aacucgauuu uccuaaaaau     2940

cgugacguaa aucauuauca ucaugccuuc gaugcguuuu uagcggcaag aauagguaca     3000

uauuuacuaa aacgcuaccc caaacuagca ccuuucuuua cguacggcga auuugcaaaa     3060

guagauguua agaaauuuag agaguuuaau uuuaucggag cccuaaccca cgcgaagaaa     3120

aauaucauug cuaaagauac aggugaaauu guaugggaua aagaacgcga cauacaagaa     3180

uuagaccgaa ucuacaacuu caaaagaaug cuaauuacac augaaguaca cuuugaaacc     3240

gcagaccuau ucgaccaaac cauuuaugca gcaaaagauu caaaagaacg agguggacga     3300

augucucuaa uaccgaagaa acuaggcuac uuaaccggua ucuauggagg auaccaaacc     3360

gaaagaggau cauauguaag uauaauuaga acuuuuaaga aaggcgaaac cauuuauaaa     3420

gucguuaaca uacuuacagc ggacguuucc cuccuaucug aaaaagaaaa aguaucgaaa     3480

acccuggcag auauuuaccu gaaagaauua aucgcuaaga aauuaucgaa aggaaaaucg     3540

gggaaaaaca uagccucgga accauuugaa auuguacuac cuagaguagg aaaagacucc     3600

uuauucaaaa acaagaaaua cggauuauuc cuuauagaau cugcgacgga acuucacaau     3660

uaucaggaau uaugguuacc aagaaaugac caacuauuaa uaaaaaggau acaagcgcga     3720

caaauugaag aaucaaaagc cgauuacgag cucacacgua uguaccgcaa uauugcgugg     3780

caaguagacc auuacuucag auuauauaaa auucgaaaau ucgcugaaaa acuacaagcg     3840

ucagucgagg auuuugcagg auuaccugua uauaaaaaug aaaccucaga cggaaaaguc     3900

gaaguauuag cuaguguacu gaaaagacua aacggggcag cagauaaagg aagaacaaau     3960

ccaaccacag ucaaacuuca gagcgacuuc ggaagaacuc uauuaaccaa cgguauuguu     4020

cuagaagaug auaguuccau aguauaucaa ucacccaccg gacuuuuuaa acgaagugua     4080

aaacucgucg accuauga                                                   4098


<210>  5407
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5407
augacaaaag aauacuacuu aggacuugac guaggaacaa acucgguagg cugggcaguu       60

acggauaguc aauauaacuu auguaaauuu aaaaaaaagg auaugugggg cauaaggcuu      120

uuugaaucag caaauaccgc caaagaccgc agauuacaaa gaggaaauag acggagacug      180

gaacgaaaaa aacagcgaau cgaccuccuu caagaaauau uuagcccuga aauaugcaaa      240

aucgacccaa cauucuucau aagacuuaac gaaucaaggc ugcaccucga ggauaaaagc      300

aaugacuuca aauacccucu cuuuauagaa aaagacuauu ccgauauuga auauuacaaa      360

gaauucccaa ccauauucca ucugcgaaaa cauuuaauag aauccgaaga aaaacaagac      420

auucgguuaa uuuacuuagc guuacacaau auaauaaaaa caagaggaca cuuuuuaaua      480

gacggagacc uccaaucugc gaaacaacug aggcccauau uagacacuuu ccuacuauca      540

uuacaagaag agcaaaacuu aagugucucu uuaagcgaaa aucaaaaaga ugaauaugaa      600

gagauuuuaa aaaaucgaag cauagcaaag agugaaaaag uaaaaaagcu aaaaaaucuc      660

uuugagauuu ccgacgaauu ggaaaaagaa gagaaaaaag cccagucagc uguaauagaa      720

aacuuuugua aauucaucgu agggaauaaa ggagauguau guaaauuuuu acgaguaagc      780

aaagaagaac uugaaauaga uuccuuuuca uuuucagaag gaaaauacga agacgacaua      840

gucaaaaacc uagaagagaa aguucccgaa aaaguauauu uauuugaaca aaugaaagca      900

auguaugacu ggaacauucu uguugacaua uuagaaaccg aagaauacau aucuuucgca      960

aaaguaaaac aguacgaaaa acauaagacu aacuuaagac uuuuaagaga cauaauucuc     1020

aaguauugua caaaagauga auacaauaga auguucaacg acgaaaaaga agccggauca     1080

uacaccgcau acgucggaaa acugaaaaaa aacaauaaaa aauacuggau agagaaaaaa     1140

aggaauccag aagaauucua caagagccuc ggcaaacuuu uagacaaaau agaaccuuua     1200

aaagaagacc uagaagucuu aacaaugaug auagaagaau gcaaaaacca cacucuauua     1260

cccauacaga aaaacaaaga caauggggug auaccccacc aaguacacga agucgaauug     1320

aaaaaaauuu uagaaaacgc aaaaaaauau uauuccuucc uaaccgaaac agacaaagac     1380

ggauacucag uaguccaaaa aauagaauca auauuuagau uccggauucc cuauuacgug     1440

gggccauuaa gcacacgcca ucaagaaaaa gguucuaacg ucuggauggu aagaaaacca     1500

ggaagagaag acagaauaua uccauggaac auggaagaaa ucauagauuu ugagaaguca     1560

aacgaaaauu uuauaacaag aaugacgaau aaguguacuu auuugauagg agaggacgua     1620

cuaccaaaac acucacugcu auacucgaaa uacaugguac uaaacgagcu aaacaauguu     1680

aaaguacgag gaaagaaauu gcccacaagu cucaagcaaa aagucuuuga agacuuauuc     1740

gaaaauaaaa guaaagucac aggcaaaaac cuguuagaau accuacaaau acaagacaaa     1800

gacauacaga uagacgaucu aucgggauuu gauaaggacu ucaagacaag ccucaaaucg     1860

uaccuugauu uuaaaaaaca aauuuuugga gaggaaauag agaaggaauc cauccagaac     1920

augauagaag auauuauuaa auggauuaca auauauggaa augacaaaga aaugcuuaaa     1980

agagucauaa gagcuaauua cagcaaccaa cuaacagaag agcaaaugaa aaaaauaacc     2040

ggauuucagu acucaggaug gggaaauuuc aguaaaaugu uucucaaagg uauaucaggu     2100

uccgauguaa gcaccggaga aacguucgau aucauaacag caauguggga aacagacaac     2160

aaucucaugc aaauccuuuc caagaaauuc acauucaugg acaauguaga agauuucaac     2220

ucaggaaaag ucggcaaaau ugacaagaua accuacgauu caacagucaa agaaauguuu     2280

uugucaccag aaaacaaacg cgcagugugg cagaccauac aagucgcgga agaaauaaaa     2340

aaagucaugg ggugcgaacc gaagaaaaua uucauagaaa uggcaagggg aggagaaaaa     2400

gugaaaaaac gaacaaaauc aagaaaagcc caauuauugg aacuauaugc cgccugcgaa     2460

gaagacugua gagaauuaau aaaagaaauu gaagacagag augaaagaga cuucaacuca     2520

augaaacucu uucuuuauua uacccaauuc ggaaaaugca uguauucugg ugaugacauc     2580

gauauaaacg aacuaaucag ggggaacuca aagugggauc gcgaccauau auauccacaa     2640

ucuaaaauaa aagaugauuc gauagauaau cucgugcucg ucaacaaaac auacaacgcg     2700

aaaaaaagua augaauuacu aucagaagac auacaaaaaa aaaugcacuc uuucugguua     2760

ucauuacuaa auaaaaaacu cauaacaaag ucaaaauacg accgacuaac aagaaaaggg     2820

gauuuuacug augaggaacu cucaggauuc auagcaaggc aacucguaga aaccaggcaa     2880

ucaacaaaag caauugcaga uauauucaaa caaauuuaua guagugaagu aguuuacguc     2940

aaaucauccc ucguaucaga uuuccgaaaa aaaccauuaa auuaccuaaa auccagaaga     3000

guaaacgacu accaccaugc uaaagacgcc uaucuaaaua ucguaguggg aaacguauac     3060

aacaaaaaau ucacaaguaa uccaauucaa uggaugaaaa agaacagaga cacaaauuac     3120

agcuugaaca aaguauuuga acaugacguu guaaucaacg gagaaguaau augggaaaaa     3180

uguacauauc augaagacac caacacauac gauggaggaa cacucgaccg aauaagaaaa     3240

auaguggaaa gagauaacau acuguauacu gaauaugcau acugcgaaaa aggugaacua     3300

uucaaugcaa caauucaaaa caaaaacggg aacagcacug uuucacuaaa aaaagguuua     3360

gacgucaaaa aauacggagg auacuucucc gcaaacacau cauacuuuuc acucaucgaa     3420

uuugaagaua aaaaaggcga cagagcaaga cauauaauug gaguaccaau auacaucgcu     3480

aauaugcuag agcauucacc aagugcauuu cuagaauauu gugaacaaaa aggauaccaa     3540

aauguacgaa ucuuaguaga aaaaauaaaa aaaaauagcu uacuuauaau aaauggauac     3600

ccauuaagaa uaagagguga aaacgaaguc gacacuucau ucaaacgcgc gauccaacua     3660

aaacuugauc aaaagaauua cgaauuagua cguaacaucg agaaauuucu ggaaaaauau     3720

guagaaaaaa aaggaaacua uccaaucgau gaaaacagag aucacauuac acaugaaaaa     3780

augaaccaac uauaugaagu acuacuaagu aaaaugaaaa aauucaauaa aaaaggaaug     3840

gcugaccccu cagaucguau ugagaaaagc aaaccaaaau ucaucaaacu agaggaccua     3900

auagauaaaa uaaacguaau aaacaaaaug uuaaaucuac uccgauguga caaugauaca     3960

aaagcggauc uaucauuaau agaauuacca aaaaacgcag guaguuuugu uguaaaaaaa     4020

aauacaauag ggaaaucaaa gauuauacua gucaaucaaa guguaacagg ccucuaugaa     4080

aaccgacggg aauuauga                                                   4098


<210>  5408
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5408
auggacacug ccaaaaccaa caaacaaccc uauuacauug gcuuagacau ggguaccucg       60

ucagucggau gggcugucac agacgcuucc uacaaugugg ucaagaaaca cggaaaagcc      120

uuauggggug uccguuuguu ugaagaagca caaaccgcag cugacagaag guuacaucga      180

acagcuaggc gucgaauaca aagaagaaaa caaaggcuug cauuauuaca agaauuauuu      240

gcaaaagaaa uaugcaaagu cgauccgggc uucuucauac gacuacacga aagcagacua      300

uggcaagaag auaaaagcau agaucaaccu aacacuuuau uuaaugauga agauuucgac      360

gacaaagcau accauaaagc cuauccaaca auauaccacc uuagauacgc cuuaaugaca      420

gaaaacaaga gcuucgacgu cagacugguu uauuuagcua uacaucauau aauaaaacac      480

cgcggccauu uccuacuaga aaauuaugac cucaguaauc aagaaacaag uggauuugac      540

gaaucuuaua uugcccugac aaacgcaauu aaagaacuuc uccaaaaaga gauuccaguu      600

ggacaugugg agaaaauuca aauaauacuu aaagacaaau cgcuaucaaa aagagagaaa      660

acagagaaaa uccuacaagu uugggacgua auaaaagaua aacaauuaaa agaaguggua      720

caauuaauau guggcgguac agcaaaucuc ucaaccuuau ucauggacga aucccuaaaa      780

gaagcacuaa aaguauuuga aaucaaagac ucaaaaauua gcuucucaac ugcgguguau      840

gaagaaaaag aaucagaccu acaaagcgua cuagaacaac gauuugaccu agucuaugcu      900

gccaaaaugc uguacgauug gucucuucua gcagaacuaa ugggcgauag aaaaacccua      960

ucagaagcaa aaauugcaau auacgaaaca cauaagaaag aucuagaagu cuuaaaacgu     1020

uuaacaagac aagauaaauc ucuauaccgc aaauuauucc gaggaaccga caaagauuca     1080

uauagcgcau acgugggaag cugcaugaua cauggagaaa aauuaguaau agagaaaaga     1140

ggcucaaccg aagaauuuua caaauuucuc aagaaagaaa uuucgaaacu accagauugc     1200

gaagauaaaa uuuacguacu cucaaaaauu gaggcagaaa acuucuuacc aaaggcagua     1260

uccaccauaa auggaauaau cccuuaucaa cuacaagagc aagaauuaga caucauucua     1320

aagaaggcgg aacaguaucu accauucuua auggaaaaag acgcauaugg aacaauaagu     1380

gagaaaauaa aacaauuacu cacauuccgu auuccauucu acguaggacc cuuaaauaga     1440

cacagcgaua aaucaugggc uguucacacu gauaaagaag gaaggauccu cccuuggaac     1500

uuuaaugaac gcauaaauga agagaaaagc gccgaaaagu ucauaacaag aaugaccaac     1560

aaaugcaccu acuuguuagg aaaagacgua cuacccaaaa acucucuauu auauacugaa     1620

uauaugcuau uuaaugaacu gaauaacguc aagaucggca aggcaggaac cuauuugacu     1680

gaagaacaaa aagaaaaacu guggaaagaa cuauuucuua cuacaaagaa aguaacacua     1740

aguaaauuca guaaauuccu aauuaaagaa ggaaucgaua aagaagaagc ggucgaaaua     1800

aaaggauuag acgguggauu uaaaucaucg uuagcaccgu ggauagaucu uaaaaacaua     1860

cuaggggaag acuuuacaag ggacaaagca gaagaaauaa uaaaggcuau cacauuauuu     1920

agcauggaua agaaaauacu caagaaauau uuaaugagaa uaguuaguga accaguugca     1980

aaacaguuau caaaacuacg auauacuggu uggggcagau ucucaaaaga guuccucacg     2040

gaaauaacac cccgagugac agacgauguc caaguacuug ucgacacaag cacuggugaa     2100

auaaugaaca ucauaacagc uuuaaagaaa acgucauuaa accuaaugga aauauuaagu     2160

ucaacguacg guuacucagc agcaauuucu gaagccaaca aagaacuggu ugguaauaca     2220

guacucagcu acgaaacagu uaaagaacug acgguaagcc cagcuguaaa aagaccaaua     2280

uggcagacac uaaaaauaau aaaagaaaua acucauauca ugggugcaga acccgcacga     2340

auauucaucg aaauggcgcg agacaaacaa ccugacaaag guagaaccga uucacguaag     2400

aaacaauuga ucgaacuaua caagaaaugu aaagaagacg uucgcucuug gagcaaucga     2460

gaaagcaaag acugggucga agaaauagaa ucaaaaucag auucccaacu ccgauccaau     2520

aaacuauuuc uauauuacac acaaaugggc aaaugcaugu auacaggcaa accuauagac     2580

cucggagcac uccuaaaggg acaacuauac gacagagauc acauauaccc gcaaucacaa     2640

acgaaagacg auagucuaga caaccuagua auagucgaca aucgagaaaa cagagacaag     2700

uccaacacau auccauuauc caaaaauauc agagaaagac aaauuggauu cuggaaacua     2760

uuaagagaca aaggauucau aucuaaagaa aaauacuucc ggcuaacuag gaauaccccc     2820

uuuuccgacg cggaaaaagc ggaauuuaua gcgagacagc uagucgaaac ccgacaauca     2880

acaaaagcag ucgccgaaau acuuaaacgu guauuaccaa aaucaauuau aguguaugua     2940

aaagcgggac ugacaucaag auucagacaa uauggagaau uuauaaaagu aagggaucuu     3000

aaugauuacc aucaugcaaa ggaugccuac uuaaauauag uaacuggcaa cguacauuac     3060

acaaaauuua cauccaaucc cauacgcuuc guaaaagauu gccaaauuua uucacugaac     3120

gaacacgccc ucuacaaaua uacgguagaa agaaauggag uaguugcaug ggccccuaau     3180

gaagaaggaa ugaugccauc cguaacgaga uggaugaaca aaaacaacau ccuagucacc     3240

cgcaugagcu auguugguca aggcgaacua uucgaucaac ucccucucaa gaaaggcaaa     3300

ggacaaguau cauuaaagaa agaaggagcg auuucugaca uuucuaaaua cggcggauau     3360

aacucugccu caacugcaua cuucauguac guugaaggag aagacaacaa aggaaaaccc     3420

gucuacguaa uagaaaccau gccccuacau uuagcagcac gccucacaac acuagaagaa     3480

aaacaaaagu auugugaaga auuauggaug uccacaggcg gcaaacuuaa aaacccuaaa     3540

guucuaauag aacaaauccc uauacaaagu uuauucucag uagacggauu cagagugacc     3600

aucgcaggaa aaacuggaaa acaauacggg ugccgauuag cagaagaacu cguacuaaac     3660

aacggauaug caaugacgcu aaaggcugua uuaaaauucg ugaaucgaag aaaaaugaau     3720

aaagaagcac uuauuacaag uuaugaccau cuagaagaaa gccaguuacu agcauuguac     3780

gacguauuuu uagacaaacu agagcacucg uguuuuagua aaagauucag cacacaaaga     3840

gauguuuuaa agaaugggcg cgaaaguuac aucgcacuau cacuagaaga caaaugccaa     3900

auacucucag aaauccuaca ucuuuuucaa ugcaacgcua cauuauccaa ucuguccuua     3960

cucggaucuu ccaaacaguc cggacgauua uacaugagca aaaaucuauc agauaaagau     4020

ucaugguaca uaauccauca aagcguaaca gguuuuuacg agcaacacau accacuagca     4080

cccuacaaga aaucauga                                                   4098


<210>  5409
<211>  4098
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5409
auggugaaaa agaaauaugc cauuggcaua gauauaggaa caaacucggu agguuggucg       60

gucgucacag augacuacaa aguacccagu aagaaaauga aagucuuugg aaauacagaa      120

aaacgauaua uuaagaaaaa uuuauuagga acauuauuau uugaugaagg caauacagca      180

gaaaaccgaa gauuaaaacg aacagcaagg agaagauaca cucgcagaag aaauagaaua      240

cucuacuuac aagaaauauu cgcagaagaa auuaacaaaa uagaugacuc auucuuucaa      300

cgauuagaug acucauucuu aauaguggaa gauaaacaag gauccaaaca cccaaucuuu      360

ggaacguuac aagaagaaaa gaaauaccau aaacaauucc caacaauaua ccaucuaaga      420

aaacaacuag ccgacuccuc acaaaaagcc gacauacggc uaauauaucu cgcacuagcu      480

cacauaauaa aauaccgagg acacuucuug uucgaaggug accuaaaauc agaaaacaaa      540

gauguccaac aucuuuuuaa ugacuucgua gaaauguuug auaagacggu ugaaggauca      600

uaccucagug agaauuugcc aaacguagcu gacguauuag ucgaaaaagu cucaaaauca      660

agacgacucg aaaacauacu acacuauuuc ccaaaugaga agaaaaacgg acuauucggc      720

aacuuuuuag cauuagcacu aggcuuacag ccaaauuuua aaaccaauuu cgaacucgcu      780

gaagacgcca aaauccaauu uucuaaagaa acauacgaag aagaccuuga agaacucuua      840

ggcaaaauug gcgaugauua ugccgaucuc uuuauugcaa ccaaaucucu cuaugacggu      900

auucuacucg cuggaauacu gagcacuacg gauucaacaa caaaagcacc cuuauccucu      960

ucuaugguaa aucgauacga agaacauaag aaagaccucg cucuacugaa aaacuucauu     1020

caccaaaacu uaagugacuc auauaaagaa guauucaacg auaaacugaa agauggauau     1080

gcaggauaca ucgaaggcaa aacaacccaa gaaaacuuuu acagauucau aaagaaagca     1140

auagaaaaga uagaagguuc agacuacuuu auagauaaaa ucgacagaga agauuucuua     1200

agaaaacaac gcacguuuga uaauggcucc auuccacacc aaauccaucu ccaagaaaug     1260

cacgcaauca uuagacgaca agcagaguuc uauccuuucc ucguagaaaa ccaagauaaa     1320

auugagaaaa uucucacauu ccgaauaccu uauuacguag gaccccucgc aagaggcaag     1380

agcgaguucg cauggcuaaa ccggaaaucu gacgagaaaa uacgcccaug gaacuucgau     1440

gaaaugguag auaaggaaac aucagcagaa aacuuuauaa ccagaaugac caacuaugau     1500

caauauuuac cagaccaaaa aguccuaccu aaacauucuc uacuuuauga aaaauuugca     1560

guauauaacg aacuaacaaa aguaaaauuu auugcggaag guaugcgaga cuaucaguuc     1620

cucgacuccg ggcaaaagaa agacauaguu aaaacacucu ucaaaaccaa acgaaaagua     1680

acagcaaaag acauaaaagc cuaucuagaa aacagcaacg gauaugccgg cguagaacug     1740

aaaggucuug aagaacaauu caaugcgagc cuaccgacau accacgacuu acugaaaaua     1800

uuaagagaua aagcauucau agacgcagaa gaaaaucaag aaauauuaga agacauaguu     1860

cuaacauuaa cacuauucga agacagagaa augauacgaa aaagauuaga aaaauacaaa     1920

gauauacuaa ccgaagaaca aagaaagaaa cucgaaaggc gucacuauac cggaugggga     1980

cgauugagug caaaauugau aaacggcaua cuggauaaag uaacccgcaa aacaaucuua     2040

ggauacuuaa ucgaugacgg aacgagcaau cgaaauuuca ugcaacuuau caacgacgau     2100

acccucuccu ucguagacga aauaagacua gcacagggau caggagaagc agaagacuac     2160

cgcgccgaag uacaaaauuu agcuggaucu ccagccauua agaaaggaau ccuucaaucg     2220

cuaaaaaucg uagaugaacu caucgaagua augggauaug acccagaaca cauugucgua     2280

gaaauggcaa gggaaaauca auuuacuaac caaggucgac gaaacucaca acaaagauac     2340

aagaaaaucg aaaacgccau uaaaaacuua gacucaaacu uaaacagcaa aauauuaaaa     2400

gaauacccca cuaacaacca agcucuacaa aaugacagau uauuuuugua uuaucuccag     2460

aacggaaaag auauguauac agacgaagaa uuagauauag accaacucag ucaauaugac     2520

auagaccaca uuauacccca agcauucauc aaagacgaua guuuagauaa caaaguucuu     2580

acaaaaagug ccaaaaacag aggcaaaagc gacgauguuc cauccuuaga aauaguucac     2640

aaaaagaaaa auuucuggaa acaauuguua gacucccagc ucauaucaca gcgaaaauuu     2700

gacaauuuaa caaaagcaga aagaggcgga cuaacgaaug aggacaaagc aagauuuauu     2760

caaaggcaac ucguagaaac acgccaaauc accaaacacg uagcuaggau uuuagacacc     2820

agguucaaca cgaaacuaga cgaagccggg aaccgaauaa gagaucccaa aguaaacaua     2880

auaaccuuaa aaagcaauuu gguuagucaa uucagaaaag acuaucaacu auauaaagua     2940

cgagaaauua acaauuauca ccacgcacac gacgccuacc uaaacgcugu agucgcuaca     3000

gcucuccuaa agaaauaccc acaacuagca ccggaauuug uauacgggga cuaccccaaa     3060

uacaauagcu auaaaucuag aaaaagcgcc acugaaaaag uacucuucua uuccaacauu     3120

augaacuucu uucgcagagu ccuaguauau agcaaaaccg gugaaguacg aauaagacca     3180

guaauugaag ucaauaaaga aacaggcgaa auaguuuggg auaagaaaag cgacuucaga     3240

acaguaagaa aaguacucuc auacccacaa guuaacguug ugaagaaagu agaaaugcaa     3300

acaggagguu uuucaaaaga aucaauacua caacacggcg auucagacaa auuaaucccu     3360

agaaaaaccg aaaaauucua ccuagauacc aagaaauaug gagguuuuga uuccccuaca     3420

auagccuaca gcguauuauu aauagccgau auagaaaaag gaaaagcaaa gaaauuaaaa     3480

cgcguaaaag aacucauagg aauaaccaua auggagagga uggcuuuuga gaaaaacccc     3540

auagaauuuu uagaacacaa aggcuauaaa aauauuuuag agaaaaacau uauaaaacua     3600

ccaaaauaua guuuauuuga acuagaaaac ggaagacgua gacuccucgc uucugcaaaa     3660

gaauuacaaa aaggaaacga aaugaucuua ccaccacauu uaguaacccu uuuauaucau     3720

aguucaaaua uccauaaaau aacagaacca auccaccuaa auuacguaaa caaaaauaaa     3780

caugaguuca aggaauuauu aagacauaua agcgauuucu caaccaggua uauauuagca     3840

caagaccgau uaucaaaaau agaagaauua uacgacaaaa augacgggga ugacauaucc     3900

gauuuaaccu cgaguuucgu caaucuucuc acuuuuacag cuaucggcgc ccccgccgcc     3960

uuuaaauucu uaggcucggu cauagauaga aagagauaua caagcaucgc ggaaauauua     4020

gaagcuaccc uuauacacca auccguaacg ggauuauacg aaacaagaau agaccucagc     4080

aaauuaggcg gcgacuga                                                   4098


<210>  5410
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5410
augaagaaac cauacucaau cggucucgau auaggaacca acuccguggg uugggcagug       60

auaacggacg acuauaaagu accagcaaag aaaaugaaag ucuuaggcaa uacaaacaag      120

aaaaccauaa agaaaaaccu aauaggugca cuacuuuuug augcaggaaa cacagccgca      180

gauagacgcu uaaaaaggac cgcgagaaga agauauacaa gaaggaggaa ccggauuuug      240

uacuuacaag aaaucuuugc cgcugaaaug aauaaacuag augaaucauu cuuucaccga      300

cuagacgauu cguuuuuagu accggaagac aaaggagggu ccaaauaucc aauauucgga      360

acauuagaag aagagaaaga auaccacaaa caauucccaa caaucuauca ccuacgaaaa      420

agccuagcag auagcaagga aaaagcagau cuuagauuga ucuacuuagc ccuugcucau      480

auaauaaaau auagggguca uuuccuauac gaggacacuu uugacauuaa aaauaaugac      540

auacagaaaa uauuuaacga guucacauca auauacaaca acaucuuuga agaaucaucc      600

cuaucuaacg aaaaugcaca agucgaagaa auauucaccg acaaaauauc uaaauccgca      660

aagaaagaua ggguucuaaa acucuuuccu gaugaaaaau cuacaggcuu auucagugaa      720

uuucuaaaac uaauaguagg aaauaaagca gacuuuaaga agcauuucga ucuggaagag      780

auggcaccau uacaauucag caaagauacc uaugacgaag auuuagaauc auuauugggg      840

caaaucggag acgacuaugc agacuuauuc guugucgcaa agaaacuaua cgacgcgauu      900

uuacuagcag gaauccuaag uguaaaagac ccagccacaa aagcaccauu aucagcauca      960

augauagaaa gauaugauaa ucaucaaaau gaucuaucag cacuaaaaca auucauaaga     1020

aguaaccuac cagaaaaaua cgcagaaguc uuuucagaug acucaaaaga ugguuaugca     1080

ggauauaucg acggaaaaac aacucaagaa ggauucuaua aauacauuaa aaaccaaaua     1140

aguaaauuug aagguacaaa auauuuccua gagaaaauag aaagggagga uuuucuaagg     1200

aaacaaagaa cauucgauaa cggaucaauu ccacaucaaa uccaccuaca agaaaugaau     1260

gccauuuuac aaaaucaagg agguuauuau cccuuccuaa aagcgaacaa agagagaaua     1320

gaacaaauac uaacauucag aauaccauac uaugucgggc cuuuagcuag gggaaacucu     1380

gacuucgcau ggcuuaccag aaacucagau gacgcuauca gaccguggaa uuucgaagaa     1440

guuguagaua aagcaaguuc ugccgaagau uucauaaaca agaugacaaa uuacgacuua     1500

uauuuaccag aagaaaaagu ccuccccaaa cacucauuau uauaugaaac auuugccgua     1560

uacaacgaac uaacaaaggu gaaauucauc gcggaagcaa ugaaggauua cgaguuccuc     1620

gacucgggac aaaagagaaa aaucguugaa aauaucuuua agaaucauag aaaaguuaca     1680

gaaaaagaua uuauuaauua cuuacauaac guugauggau acgacggaau agaaauaaaa     1740

ggcauagaaa aacaauuuaa cgcaucauua uccacauacc augaccucuu gaaaauaaua     1800

aaagauaaag aguucaugga uaauccuaaa aacggagaaa uacuagaaag cauaauccac     1860

acccuaacca uauuugaaga cagagagaug auaaaacaac ugcuagcaca guauaauuca     1920

guauucgacg aaaaaguaau aaaagcacua aguagaaaac auuacacugg uuggggaaaa     1980

uugucagcaa aacuaaucaa uggaaucaga gacaaacaga cgggaaacuc aauucuagac     2040

uaucuuaugg augaugggaa aucuaaucgg aauuuuaugc aacuuauuaa ggaugauggc     2100

cugagcuuua aagaaauaau agauaaggcc caaguugugg gcgaaacaga uaauuuacgc     2160

caaguaguac aaaaccuacc aggaucccca gccaucaaga aaggaaucuu gcaaucgauu     2220

aagcuaguug acgaauuagu uaaaguuaug ggaucagacc cagaauccau cguaauagaa     2280

auggcaagag aaaaucaaac aaccaacaaa gguaaaucca aaagccaaca aagacucaaa     2340

acacuaagcg acgcgauauc ugaauuaggc agcaacauuu uaaaagaaca cccuacagau     2400

aacauacaau ugcaaaacga cagacuauuc cuguacuauc uacagaacgg caaagauaug     2460

uacacaggug aagcacuaga uauaaaccaa uuaucaaauu acgacauaga ccacaucaua     2520

ccucaagcau uuauaaaaga cgauucacua gacaauagug uguuaacguc aucaaaagac     2580

aacagaggua aauccgacaa uguaccuucg uuagaaauag uagagaaaau gaaaggcuuu     2640

uggcaacaau uauuagauuc aaaauuaaua agcgaaagaa aauuuaacaa cuuaacaaaa     2700

guagaaaggg gagguuuaga ugaacgagac aaaguaggau ucauaaaacg acagcuagua     2760

gaaacaagac aaaucacaaa acacguagcc caaaucuuag acgcaagguu uaacgccgaa     2820

guaaaugaga aaaaucaaaa auuacgaagc gucaaaauaa ucacacuaaa gagcaauuua     2880

guaucuaauu uccgaaaaga guucggcuua uauaaaguca gggaaauaaa cgacuaucac     2940

cacgcacaug acgcauaccu caacgcggua auagcgaaag ccauacucaa ggaauauccg     3000

aaauuagaac cagaguucgu cuacggugac uaccaaaaau acgaccuaaa acgauauaua     3060

ucccgaacua aagauccaaa ggaaauagaa aaagccaccg aaaaauauuu cuuuuauucu     3120

aauuuacuaa acuucuuuaa agacaaagug uauuacgcag acggaaccau aauccaaaga     3180

gggaauguag aauacucuaa agacaccgga gaaauagcau ggaauaaaaa gagagacuuu     3240

gcuauagucc guaaaguauu gucauaucca caaguaaaua ucguuaagaa aacagaagaa     3300

caaacaggag guuucucuaa agaaaguaua cuaccaaaag guaacucaga caaauuaauc     3360

ccaagaaaaa cuaaaaauau ccaacuagac acaacaaaau acggaggauu ugauucucca     3420

guaauagcuu auucaaucuu auuagucgca gauguggaga aaggcaaauc gaagaaacuc     3480

aaaaccguca aaucacucau aggaauaacc auuauggaga aagucaaauu cgaagcgaau     3540

ccagucgccu uccucgaagg caaaggcuau caaaauguag ucgaagaaaa uauuauacgu     3600

cuaccaaaau acaguuuauu cgaguuagaa aacggccgaa ggagaaugcu agcaucaagu     3660

aaagaacuuc aaaaaggcaa ugaaauggua uuaccaaguu aucuauuagc gcuacucuau     3720

cacgcuaaga aaauccaaaa gaaagacgaa ccagagcauc uagaauacau aaaacaacau     3780

cauucagaau uuaaugauuu acuuaauuuu guaucagauu uuucacaaaa auacguuuua     3840

gcagaaagua auuuagagaa aauuaaaaac uuguauauag auaaugaaca aacaaaucua     3900

gaagaaauag caaauaguuu uauaaaccua cuuacuuuca cugcauuugg ugccccugca     3960

guuuucaaau ucuucggaaa agacauagaa cgaaaacgau acuccaccgu aaccgaaaua     4020

cuaaaagcaa cgcucaucca ccaaucuauc acaggcuuau acgaaacgag aaucgaucuc     4080

agcaaacuag gagaagaaug a                                               4101


<210>  5411
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5411
auggauaaga aauauaacau aggcuuagac auaggaacau caucaauagg cuucguagca       60

acugaugaag auaaucacgu uauacgcgca aaaggcaaaa acguaauugg agucagauua      120

uucgaugaag gucaauccgc ugcagaaaga agaggauuua gaacaacuag aaggagauua      180

aaucgccgaa aauggagacu aaauuuacuc agagagauuu uugaacccca cauauauccu      240

auugaugcca guuucuuuau gagacaaaaa uacucaaacu uaaguaaaca agauccaaau      300

cagaaaauag aguuuaaaga cauacuauuc ucagauaaau cagaugcaga guucuaccga      360

caauacccua cgaucuauca cuuaaggcaa gcccucauga ccgagaaaag acaauuugau      420

cuccgagaaa uuuauuuggc aauacaccac auuguaaagu acagggggca cuuuuuagau      480

gaaaccccua cagauaaauu cucuuuugac caacucgacc uaacccaaga auuugaacgu      540

cucaauguac ucuuuagcga acaaaacccu gaagaaccau uugaaauagu uguaaaccaa      600

aaccaaagca uagaagauau ccuuucaaac caauccauga cagcauccga ucgaagaaaa      660

caagccuuag uaguauucua ucaacaaagc gaaaauaaag aaaacgaaaa gcaauacaaa      720

gcaauagcua cagaaauauu aaaucuacuu uuaggaaaca aagcgaaacu uaaugucauu      780

uuagcucaag aagucgacaa agaagcagcc aaagauuggu cacuaucauu aucaagugaa      840

acauuugacg acgacuuaga aaaagccuca ucaggacuaa acgacuccca auuggaaaug      900

guuggaauau uugaacgaau auauaguucu cugauucuaa aaucaauaau uggagaccau      960

aauucgcucu cugcagcuau gguagauaaa uucaaaaccc acgcgcaaca cagggaccuc     1020

uugaaggacg ucauuaaaaa uucagacgcc acaaaaggcg aacaacuuca guucgcuuac     1080

gaccacuacc uucaaagaca cagcgccgac gggaaagcau auggaaaaga cgacuucuau     1140

aaagaaguaa agaaaaaucu agacgauagu gaugcugcac aacaaaucca acaacuaauc     1200

gacacagacc aauucaugcc aaaacaaaga acaaaagcca acggugcuau uccucaucaa     1260

guccaacaaa aagaacuaga cuuaauaaua gaaaaucaau gucaauauua cccauggcua     1320

gcggaagaaa auccgaauag cgaaagacau aauguagcca aauauaaauu agaugaauua     1380

gugucuuucc gaguuccaua cuacguuggg ccgcuaauuu cgccggcaga cgaugaaaca     1440

gauccccagg ccauccaaaa uacaaaauuc gcauggauga uacgaaaaga ugcgcaaggc     1500

agaauaacac ccuggaauuu cgaugacaaa guggauagag aaaaaucagc agauaaauuu     1560

auaaaacgaa ugacaacaac agauaccuac cuuaucggag aagacguccu accagaucag     1620

ucccuaauau accaaagaua caaaguccua aacgaacuaa acaacguaag aaucaacgga     1680

cgacccauag aaguccaaau uaaacaaaac auauauaccg accuauucaa gaaauucaaa     1740

ucggucucgg ucaagaaauu aagauccuau cuugaggaaa uguccuauua uccaggcuca     1800

uucaaaauau caggauuaac gaacccaggc aaauucgaca auggacuuac uucauaucuc     1860

gauuacaaaa acauccuagg agacucuguc gacgcuaaug auaaaagagc ggacauagaa     1920

caaaugauag aauggucaac aauauucgaa gauaauaaaa uauuugcaca aaaacucgag     1980

ucaaugacau ggcucaccgc agaacaacgu aaagcaauau caagaaaaag auacacgggc     2040

ugggggagac ucucaaauaa acuucucaca caacuuaaaa accaagacaa cagaucuauu     2100

auggaucuau uaugggagac agaagaaaau uucaugaaaa uacaauccgc accugacuuu     2160

ucuaaaauga uagaagcgca uaaucaacaa uuuaucaaaa acgaagcaaa cauccaacaa     2220

guaaucagcg aucuuuacac aucuccacaa aauaaaaagg cuauaagaca aauacuauua     2280

guuguagaug auauacaaaa agcagucgga caugcuccag agcauaucgc uaucgaauuu     2340

gcaaaagggg aagacaaaaa ucccagaaga acuauaaguc gagaaaugaa aauucaagac     2400

guuuaccaac aaaucucaga ugauaucuua gucucgccgg aaguacgagc acaacucaau     2460

gagaaaauag aaaauagaca cggacgccuc agugaccggc uauuuuuaua cuuuaugcaa     2520

ggaggaagag acauguauac uggcaaaccu auuaacauaa acaauauaag ucauuaugac     2580

auagaccaca uccuacccca ggcuuucauc aaggaugacu cccucgauaa uagaguauua     2640

gucucagaca agcucaacag agaaaaauca gauacagugc caagagacuu guuuggccga     2700

gaaaugaugu cuaccuggga gaaaauggca gacagaggcc uaaucacgaa aagaaaacug     2760

gcgaacuuaa aaacaauacc uggaagcauc gacgcacaca aagcacaagg auucauaaac     2820

agacagcuag uagaaacaag gcaaguaauc aaacuugcug uaggaauaau gauugaccaa     2880

uauccugaaa cacaaaucgu aagcgucaaa gcuaacuuaa cccacgaaau gagggagaug     2940

uuugaccucg uuaaaaaccg agacgugaau gacuaccacc acgcuuucga ugcauaucuc     3000

acugcauucg ucggaaacua ccuauuacaa agauauccga aacuugagcc cuacuuugua     3060

uaugauaaau ucaagaaauu aaaagaucaa aacauaagua ugcgugacuu uaacuuacug     3120

uacgacuuga agaaaaauga ggacguauac gaccaacgua caggucacuc agcacacaaa     3180

auaugggauc aacacggguc uauagacuau auuagacacc uauucgcaua uaagaaaaua     3240

cuuguuucuc acgaaguaca ccaaaacgga ggcgcauuau auaacaugac aguauaugga     3300

aaagcagaug acgcaaaaag aaaacugaua ccaauuaaag ccgauaaacc aacagaacuu     3360

uauggcggau auaccucaag gaaacuagcc uacauggcaa uaguaaaaac aaagaaagga     3420

aagaaagaac aauaccaagu aacaggaguu ccaguuagcg cacuaacaac ccuacgacaa     3480

uuuaaagaag acucaccaga acaaaaacaa gcauuagaag aaauaguuag aaggaauuuu     3540

gaugagaaaa cagaaguaga aauaauagua cccaaaguac ucuuuaauca auuaauagua     3600

gacgaaaaau guagauucau gguagggagu gauacauacc gguacaaugc acaacaauua     3660

guacuaucag cggacgaucu ggaaaucauc aaugcuucuc auaaagacau acaaacgauu     3720

gauucauccg auuacucaca acaacuggac caaguauauc aaaauauucu cacuauagua     3780

gauaaacauu uuccuaugua cgacauaaac aaauuuagaa caaaacuaca ugacauuuau     3840

gaacaauaua uagaacuacc uaaugaagac gugugggaaa auaacaaauu aacaacacua     3900

ggcaaacaua cuaucauuau aagaauccuc caagguuuac augccaacgc cacaguagua     3960

gaucuaaaac aacucggaau aaaaacucca uucggcuuua ugcagcaggc ccgcgggauu     4020

guagugagcc cggaugcaca auuaauauac caauccccaa caggacucua ugagagacgc     4080

guucgguuag gagacuugug a                                               4101


<210>  5412
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5412
augggcgaua gaaaauacaa uuuagggcua gacaucggaa cauccaguau cggauuugcc       60

gccguggaug aaaacaauca gccaaucaga gucaaaggua aaacugcaau cggaguucga      120

cucuucgaag aaggaaaaac agccgcggau agaaggggau ucagaacaac ucgaagaaga      180

uuaucuagaa gaagauggag aauaaauuua cuaaacggaa uauuugacgc acacuuagcu      240

gaaguagauc ccacauucuu agcuagacug aaagaaagua auagaucaaa ccuagauccc      300

aagaaaucgu uccaaggcag ccuucuauuc ccugaacgaa aagacuacca auucuacaaa      360

gaguauccca cgauuuacca cuuacggaaa gcauugaugg aagaagauag aaaauucgau      420

auccgagaaa ucuauuuagc aguucaucac auaauaaaau acagagggaa uuucuuaaac      480

ggcacaccaa ugaggucauu uaaaguagaa aauauagaau uggacacacu cuucgacagg      540

uuaaaucaau uauaugaaga aauaauaccg gacaaugagu uggcuuucga ccucgaacaa      600

guggcagaug ucaaagaagu acuuagcuca acaacaauau auaaaaugga caaaaagaaa      660

caauuaguaa aaaugauguu auuacccacc aguaacaaag cucuacaaag cgaaaauaag      720

aaaauaguca cccaguucgu aaaugcaauu cugaauuaua aauucaaacu agaugugcua      780

cuacaggucg aaacagaugc cgacuggucu cuuaaauuaa augacgaagg agcagacgac      840

aaauuagaag aguuuacagg agacuuagac gaaaacagac ucgaaauaau agaccucuua      900

cagagacuac acaauugguu uucauugaau gaaauaacca aagacggaaa uucguuaagc      960

gcugccaugg uggaaaaaua uaaagaucac agacaucacc uagaauuacu uaagaaagua     1020

auagaagacc auccugacgc aaagaaagca aaagcgcuga aagaaacaua uacagcauac     1080

guaggaaaaa cagacgauaa aacgcaaaac caagaugacu ucuauaaagc cguagagaaa     1140

aauuuagacg acucgccaga cgccaaagag auuaaacgcu uaauacaacu agaucaauuu     1200

augccaaaac aaagaacagg gcaaaauggc gccauaccac aucaauuaca ccaacaggaa     1260

cuugaccaaa ucauagaaaa acaaucuaaa uacuacccau uccuggcaga accaaaccca     1320

aauguaaaaa gacgaaagga ugccccauau aaacuagacg aauuaauagc guucaaaauc     1380

ccauacuacg ucggcccauu aguuacccca gaagaacaag cucaaaacgg ugaaaacgua     1440

uuugccugga ugaaacggaa aguugcagga cccauaaccc cauggaauuu cgauaagaaa     1500

guagaucgaa uggaguccgc aaacagauuc auaagaagaa ugacgacaaa agauacguac     1560

cuauucggug aagacguacu accagcagaa uccaugauau augaaaaguu cguuguacuc     1620

aaugaauuaa acaaucuaaa aauuaauggc aggcaucuau cacugaaaga caagcaagac     1680

aucuacaacg acuuauuuaa acaacagaaa acuguaucua ucaaagcacu ccaaaauuac     1740

uacguaacaa agaagaaagc agcaaccgca ccaacaguug ggggucuagc ugacccaaag     1800

aaauuucuuu ccucacuuuc aaccuauaua gauuucaaaa acauguucgg agaacagguc     1860

aaugacccac aauuccaaga agaccuagaa caaauugucg aaugguccac aauauuugag     1920

gaccgcggca uauuuaaagc aaaauuacaa gcacuaggau gguuauccga aaaacaaaua     1980

caacagcuag uugccaaaag auauaaagga uggggaaggu ugucaaagaa auuacuaaca     2040

ggccugaaaa acgcagaagg auacucaaua cuagaugaaa uguggagaag caccgaaaac     2100

uucaugcaaa uacaaagccg accagaauuu gcagcacuua uacaacaagc uaaugaaaaa     2160

caauucgagg guaaugaccc uaauaaugua ugggaaaaua ucgaaaacau auugggugac     2220

gcauauacga gcccucaaaa caagaaagca auaagacagg uagugaaagu uguacaagac     2280

auagaaaaag caguaggaaa cccacccgag aaaauugcca uagaguucac cagggaagca     2340

gcagcaaacc cgcaacgcac ucaaucacga uuaagaacau uagaaaaacu auaugaaucu     2400

gcagaagaag uaguugaugc aagacuuacg gcagaauuag cagaguucaa agaaaacaaa     2460

cacguauuau ccgacaaaua uuauuuauac uuuacacaau uagguagaga uguauacaca     2520

ggagacacca uaagccucga cagacucaac gacuacgaug uagaucacau acuuccacaa     2580

uccuucauaa aagaugauag uuuagacaau cgaguuuuaa ccauaagagc aguaaauaau     2640

ggcaaaucag acaauguacc ugccaaaaug uuuggaaaga aaaugggcag uuuuuggaga     2700

uaucuacuag auaacggcau gaucaguaaa aggaaauaca acaauuuaau aacagaccca     2760

gacaauaucu ccaaauaugc acaaaaagga uuuauaaauc gacaguuagu ggaaaccagu     2820

caggucauaa aauugacugc gaauaucuua aauggaaucu acgauaaaga uacagaaaua     2880

auugaaguac cugcaaaaau gaacucacaa augagaaaaa uguucgaucu agucaaagua     2940

agagaaguaa acgacuacca ucacgccuuu gacgcguacc uaacaauauu cauuggcaac     3000

uaccuuuaca aaugcuaucc caagcugaga ccauauuuug uguauggaaa auucaagaaa     3060

uucagcgaua acgaagacau agaagucggu gacggaagau ucaauuuccu aaauagaaua     3120

gaacgacuua aaaauguaac agauccagaa acaggagaaa uauuaugguc aaauguugca     3180

ccaaacgaaa cuauaaaaag aauaaagaaa guauauaacu auaaauuuau gaucgugucu     3240

cgggaaauca cuacaagacg cgccgaacua uucaaccaaa ccgucuaucc gaaaaauuau     3300

cgaggaaaac uaauuccaau caaagaagac cguccaacag acuuguacgg gggauacagu     3360

ggaaacacug augcauacuu agcgauagua gcacucgaag auaaaaagaa aggaaaauau     3420

uucaaaguug ucggaauacc aaccagagcg gccgcuaaac uagagaaauu aaaacaacag     3480

gauucucaac aguaucuuca agcaguccac gaagucauag caccucaauu uacaaaaagc     3540

acuaagaaag gaauaaagaa aacugaguuc cgcauaguac uagauaaagu ucauuauaga     3600

caguuaguuc aggacggacc agugaaaaug augcuaggaa gcuccacaua uaaauauaau     3660

gcaaaacaac ugguguuauc agaaaaagcc cuacagguaa uaaaagacga ccguaaauuc     3720

gaugaaaccc aaaaagauga caaccuuaua gccguuuacg augaaaucuu aaguauugua     3780

aaccaaucuu ucgacuuaua cgauaucaac ggauuucgga agaaacuaaa cgacaaucga     3840

aaucaauuua uagaccuucc ugcagaaaca aaauacgaag gccguaaagu aguagcacau     3900

ggaaaacgag aaaugauacu agaaauacuu aaaggacuuc augcaaacgc uaguuuuggc     3960

aaucuuaaac caauuggauu cgcaacucca uuuggacaaa ugcaauccaa aaaugguaua     4020

gaauuaucag aaaaugcauu cuuaauacac caaagcccca guggucuauu ugaacacaaa     4080

auuaaacuuu ccgaucugug a                                               4101


<210>  5413
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5413
augacaaaau uaggaaaacc auauggaaua gguguagaca uuggauccaa uucaauagga       60

uuugcagcag ucgaugaaaa uucccaccua auaaggcuua aagggaaaac aguuauagga      120

gcaagauugu ucgaagaagg aaaagcagca gccuauagga gagcaucaag aacuacaaga      180

cgacgacucu ccagaaacag auggagauua uccuuucuua gagacuuuuu cgaaucacau      240

auaacaccaa cagauccuaa cuuuuucaug cgucaaaaau auagugaaau aucaccaaaa      300

gauaaagcuc gauauaaaua cgagaaaaga cuauucaaug acagaacaga ugcagaauuu      360

uaccaacaau accccaccau guaucacuua agaacaaggu uacucacuga uccaucaaaa      420

gcagacguac gagaaauaua cuucgcaaua caccacauac uaaaaucuag aggacacuuc      480

cuaacuccag gguacgcuaa agacuuuaau acuaacaaag uugcacugaa ugaaauauuc      540

ccagcgcuac aagaugcaua cgcccaggua uaucccgauc uugacauaac auuugacgaa      600

aacaaaguca augaauuuaa aacaguguua cuaaacgaaa aagccacccc cuccgauaca      660

caaagagcgc uagucaacuu gcuauuagcc aaagagggug accgagacau auuaaaacaa      720

caaaaacaag uccuaacaga guucgcgaaa gcaguagugg gucuuaaaac uaaacuaaac      780

guagcauuag gaacagaagu ugacucuagu gaagcaacag cuuggaauuu cagcuuagga      840

caguuggaug acaaaugggc agguauugaa ucggcuauga cugacgaagg aacagagauu      900

cuagaccaga uucgagacuu auacagggcc cggcuauuaa acggcauagu cccagcugga      960

aaaacacucu cacaagccaa aguagaugac uacgcacaac accacgaaga ucucgaacua     1020

uuuaaagauu accuaaaaca acucgaagau gauggaacag ccaaagcuau ucgacaacug     1080

uacgaccgau auauagacgg cgaugaugcc aaacccuuug uaagagaaga cuucgucaaa     1140

gcacuaacaa aagaaguuac cgcccacccc aacgcaaaaa gcccagaacu auuggagcga     1200

uuagcccaac cugauuucau gcuaaaacaa agaaacaagg ccaacggcgc aauaccggua     1260

caaaugcaac aaagagaacu ugaucaaauc aucaaaaacc aagcaguuua uuacgauugg     1320

uuagcagccc cuaacccugu cgagaaacau agacaaagua ugacuuacca acuugacgaa     1380

uuacuuaauu uucgaauacc auacuacguc ggcccccuag uaacagcuaa ggaacaaaag     1440

gcugcacaag ggggaguuuu cgccuggaug gugagaaaag auccagaugg aaacaucaca     1500

ccauacaacu ucgacgaaaa agucgauaga gaggccagcg caaacacauu cauacaacga     1560

augaaaacua ccgauacaua ccuaaucgga gaggacguac ucccaaaaca aucacuauua     1620

uaccaauccu augaaguuuu aaaugaacuu aauaacguuc guguaaauga uaauaaacuc     1680

aguuucgauc aaaaacagca aguacuaggu gaguuauucg aacgacacaa cacagugaca     1740

auaaaacaau uuguugauaa cuuacuagca cacggagauu acgauagaau accagaaguu     1800

aggggccuag cagacgagaa aagauucuug ucaagccuau caacauacaa ucaauuaaaa     1860

ggcauucuac acgaagcgau agaugaugca gauaaacaaa cagacaucga aaauauaaua     1920

gcauggucaa ccauauucga agauaaagca aucuucaaaa caaaauugaa cgagauaagc     1980

uggcuaaacg cgggaaugau caauucccua gccaacauac guuacagagg auggggacaa     2040

uucagucaca aauuacuaaa ugaauuaaca uuauccacug gaagaacagu auuugaagaa     2100

cuacucaucu caucaagaaa cuugaugcaa auuuugacug acgaaguacu aaaacagaaa     2160

auugccgaau uaaacgccca cgaauuaaaa gcaagcggga uauaugcagc uaucgaugac     2220

gcguacaccu cucccaguaa caagaaagca cuacggcaag uacuaucggu aauagaugau     2280

auaacaaaug cagcaggaca agcuccgaac uggcuuuaca ucgaaaccgc agauggagac     2340

gguguucccg gcaaacgaac uaauucaaga caaagaaaaa uacaagaaau uuaugcuaau     2400

gcagcacaag aacuaaucac cgguucagua agaggagaau uugaagacaa aaucgcauca     2460

agagcugauu uuaccgaucg cuuaguacua uauuucaugc agggugguag agacauauac     2520

acaggagaac cacucaauau agacaacuua ucaucauaug acauugacca cauucuacca     2580

caaucacuca uaaaagauaa cucacuagac aacagaguuc uuguccaccc ucuuguuaau     2640

agagaaaaag acaccauauu cgcauccaca agauucgcca aacaaaugaa uggaaugugg     2700

cggcaguggc acgcggcagg acucauauca ucaaggaaac uccgaaaccu acaaaugcga     2760

ccagacgaaa uugacaaaua ugcuagcggg uuugucgcaa gacaacuggu agagacaaga     2820

caaauaauca aacuaacaga acaaauaguc gcagaccaau auccagacac aaaaauuaua     2880

gccguaaaag caggacucuc aucacaacuc agaaaagaau uagacuuccc caaaaaccgc     2940

gaagugaauc auuaccauca cgcuuuugac gcuuuucuug cagcuagaau uggaacauau     3000

uuacucaaac gauacccaaa ucucgaacca uuuuucacau acgguaaauu uaagaaaaca     3060

gaagugaaga aacuaaaaag uuuaaaauca cuaaaaucau ucaacuuuau cagagauaug     3120

acgcacgcga aagauaaaau uguagcaaag gaaacaggug aaauuguaug ggauaacgca     3180

ucugauauua acgagcucga uagaauuuau aacuucaaac gaauguuaau uacccacgaa     3240

guccgcuuug aaacugcuag ccucuuuaaa caaacucuuu augcagccaa aaauuccaaa     3300

aacaggggag gaucuaggca acuaauacca aagaagaaag gauaccuggu ugacauauac     3360

ggggguuaua cacaagaaac uggaagcuac uuaaguguag uacgacuuac aaagaaagca     3420

auguaugcug uagucaaagu cagcacaaga gacgcagcaa aauuagcagu agccaaaucg     3480

aucagcgaac aaaaagaaaa cgaaacgcua aagaaaauca uagauggaaa acuaucaaaa     3540

accucaaaga aaggaaaaac aacacaucaa cucuuugaaa ucguacuacc aaggguagga     3600

cagaaaacac uauuuaaaaa uucuaaauac aaucaauucc uagucaauag cgauaccuau     3660

augcauaauu aucaagaacu auggaugcca agagaguacc aaagaaugug gaaagauaua     3720

cuauuaucca aucauggaga ugcacaaauu gaaggacaac uggaccaaau auuuaaauuc     3780

aucguuucgc aaguaaacuc auacuuuaau cuuuacgaca uaaaccaguu uagaaagaaa     3840

auagcagacg cuacugacaa auucgcaaaa cuacccacac acgacacuga uaacgcaaga     3900

ggcaaaauag caacaaucgg ucaacuauua aucggacugc aagcaaacgc aaugcgauca     3960

gaucuacgga accuggauau caaaacaccc uucgguuuau ugcaaaucgg cacgggaauc     4020

acacuagauu uagauacauc gaucauuuac caaucaccaa caggacuauu cgaacgagaa     4080

guaccucuua cagaucuaug a                                               4101


<210>  5414
<211>  4101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5414
augacuaaag acuacucauu aggacuagac auaggcacag gaagcguagg uuuugcagcc       60

uuagauacga acuaucgauu acuacgagca aaaggcaaca auguaauugg aguccgacua      120

uucacucccg cagaaacagc cgaagaacga cgcggcuucc gaacagcaag acgaagauua      180

ucaagaagaa gauggagacu aggagcccuu aacgggauau uugcaccaga guuagcaaaa      240

guugacccua acuuccuaca ucgauuaaaa uacgcuuggg ugcauccagc agaccaacaa      300

aacaagggga aauacucagc cgcuccauua uuuaacgacg cagcuuugga ucgacaauuu      360

cacgacgacu auccaaccau auaucacuua cgcuuaaaac uaaugacaga caccucacaa      420

caugaucuac gcgaagucua ucuugcuaua caccauauaa uaaaauuuag gggaaacuuu      480

uuacgaggag aaggaccaau aaacgcuucu gaggcuuucg acaucaauac cuuuauagau      540

caaaugacag cuuucuacga ucaggcuuuu cccgauuuua aaauugucga ccaaguagac      600

caacaaauua uuuuuaaagg auuaaccgac augacgcuau cuccagcaga uagagcuaau      660

aaagcacuca ugggauuuaa uaaugcagca caacaaccua auaagcggau uuuuaaagcc      720

auccuaagcg gcuuaguagg caaucagguc gaccuaaugg caaucuuuca auuagauggc      780

auuccaaaag aagaccaaaa agaguucaag uuuaaauucg ucgacaauga cauagaagaa      840

aaacuagccg cauuaggaga caauccagcc cuuucagaug aacaaaacga guuccuaaca      900

acauuauacg cggcauuuag uggacuuacg cucaaaggac uucuagguga cgccaccuca      960

auuucugcag caaugguagc acguuacgca ucacauagaa cggauuggac auauaucaag     1020

gaccacguuc ucacaccaca aaacaaccga gaauuaagac uugccuacgc ugaucuuaug     1080

acagacaacg aagacguaag acuaaaagca ggaaagaaau uugccaaagc cauagaagca     1140

gcagauauac ccgcagcuaa gaaaagugaa uuuucacuaa aaauagauaa caaugaguuc     1200

cuuccacguc aaaggacaaa agcuaauggc acaauccccc aucaacuuca cuuaaaugaa     1260

cuacaacaaa ucauugaaaa ucaagggcaa uacuacccuu uucuaaaagc gacauuugaa     1320

caggacgguc auacauuaaa caaaauagaa gguuugguaa aauucagagu accauacuac     1380

guggguccgc uaguuccaaa acacaaagug gcagguaaua augcaaauca uuggauggua     1440

ccaaaagaac ccggagcaau cacaccaugg aacuacacug aaaaaguaga cagagauaag     1500

ucaggcaacg aguucauucg acgaaugacg ggaacagaca ccuaucugcu aggggaaccg     1560

acacuuccua aaaauagccu uguuuaucaa gaauuuaacg uccuacaaga acugaauaac     1620

uuaagaauag aaaagaggcg auuagauauc gcagugaaac aagauauauu cgaacacguu     1680

uuuaagacua auaaaacagu gaccguaaaa cgcauagaag aauauuuaac aacagaauuu     1740

ggcucgccag ucacacuauc aggauucgca aauguaaaug aaagaaagcu gaacucaucu     1800

uuaucaacuu aucacuacuu cguaggaauu uuuggagcug aauuuguaga cgauccuaaa     1860

aauuuaaccc uauuagaaca aauaguugaa cuacaaacug uauuugaaga ucguaaagcc     1920

uuacaaagac aguuaaaacu ucguccagaa cucucggaaa aacaagucaa acaacuggca     1980

cgcacccauu auacaggaug gggaagacuu ucucaaaaac uguuaaccag uaaaauagua     2040

cacguagccc ucgaagguga aagagaacca ggacaauaua guauacuuga aacucuauau     2100

gguacccaga ugaauuucau ggaaauuguc aacgauauga aggacagaua uggaguuaaa     2160

agcuggcuag accaggaaaa cucggguuca gaaaacaacc uaucuuuaaa acaaaggauu     2220

uuagauaugc caggacccaa aaauaucaag agaggaauua accaagccuu ucacauaaua     2280

gacgaaauaa cgagagcugu aggaaaugcu ccaucucgua uauacauuga auuugcaaga     2340

gaaagucaac agucaaaucu gacaaacgca agggucaaca gacuuaaaca acuguacuca     2400

aaucaagcau uaaagaaaga guucgcgaaa cuagcaagcg aaaucaaaga uuuaacaaaa     2460

gaacaacuaa aagacgaccg uuuauaccua uacuauuuac aacaaggaaa agacauguac     2520

acagaaacug acauagacau aacaaaaaua ucaucaaacu accagaucga ccauauaauc     2580

ccacaggcuu acaccaaaaa uaauucacua gacaacagag uauuagucaa cagugaaaug     2640

aacgcacgaa aauccgauuc gccuacauuu uugcccucau uaauucaaga acgaaaagca     2700

ugguggaggc aacuaagaga cacagguuua augucgacaa uaaaauauaa caacuuaacu     2760

cgaaccucug acgacuucag ugcagaucaa aaagaaagau ucauagcaag aucgcuugua     2820

gaaacuaggc agguuaucaa aaacguagcc acauuaauaa aagaauacua ugacgacagc     2880

caaguagaag cauuacccag cgaaauaacu gcagaaauga gaagauacuu aaauguacgu     2940

aaaaaccgag auauuaacga cuaucaccau gcacaugaug cuuugcuuuu cgcaacggcu     3000

ggcgaauuuu ccagaaaagu aggcauaaug gaaaaaggac gagucucaga uggugcuggg     3060

aacauauaua aucgauacac caaagauaua auccaaagag caagagcaaa agcaggacaa     3120

gacaagaaag uaagacccuu uacauuuaua guaggaucua uggcaaaucc agacacuaaa     3180

uuucaaacua aucaaacuac cggagaaaua guauggacac cagaagacgu agcuuaccua     3240

aggaaaguaa uugacuacaa gaaaauguua gugacaaaaa ugacuuuaga ucaaacaggc     3300

acauuguaca augaaacuag auacggcuca ggagauaaac auguugccgu ggcgcuaucg     3360

aaagauaaag cugcugaucu cuauggaggg uuuggaucuc uaaaaccggc auaugcugcg     3420

cuuguauaca guaaaaagaa auucaggcua guaaacgugu uaagacuuug guuacccgaa     3480

auaaagaaaa augccucagu acucgaacgg aaaguacaau cuguauuagg agacgauaau     3540

gcaaaaguga uacgagccca cgucccaugc ggucaauuau uaauaaaaga uggagcauua     3600

guaacaguua gcucugccac cgaauuacau aauuuuagac aacuauggcu uccuaaagca     3660

gacuaccaag auauuuccau ccuucuugga gcuccugaca aagugacagc agaaaaucgu     3720

cuacaagcgg aaucacauuu cgacgaugua gaucaaagau uugaaaguuu acuuaaagau     3780

guaauugauc aagccaggca auauuaccca cuaaaaacac aucaaacauc ccuggaaaaa     3840

cuaauaggga aaauaggaaa auucgacgaa uugaaauuug cacagaaaca acaaguauuu     3900

aguagacuuc uaagugcauu gcaugcuaac gcuacaaauu cggaucuaaa acaaauaggc     3960

cuaucaacag caugggguag acuaauuuau ccaaaagggg uuucacuaac agacgaagac     4020

aaauuaauaa auucaaguuu uacuggacuu uucaacgaug uaaugucgau uaaagaauuu     4080

caugauuuuu caauaaacug a                                               4101


<210>  5415
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5415
augaaaaaac aaaaauuuuc agauuauuac cuagguuucg auaucggcac caacagcguu       60

gguuggugua ucacagaccu agauuauaac gugcuaaggu ucaacaaaaa agacaugugg      120

ggauccagac ucuucgaaga agccaagaca gcggcugaaa ggcgcguaca aaggaacagc      180

cgccgacgac ugaaacgaag gaaauggcga uuaaaccuau uagaagaaau cuuuucuaac      240

gagauauuaa aaauagacag caacuuuuuc cgaagacuaa aagaaagcuc auuauggcua      300

gaggauaaaa gcuccaaaga aaaauucaca uuauucaacg acgacaacua caaggacuau      360

gacuucuaca aacaauaucc aacaauauuu caccugcgaa acgaacuaau aaaaaaccca      420

gagaagaaag acauccguuu aguauaccua gcgauacaua gcauuuucaa aagcagaggc      480

cauuuccuuu ucgaagguca aaacuuaaaa gaaauaaaaa auuucgaaac auuauacaac      540

aauuuaauug ccuuucugga agauaacgga auaaauaaga ucauagacaa aaacaauauc      600

gaaaaguuag aaaaaauugu augugauagc aaaaaaggau uaaaagacaa agaaaaagaa      660

uuuaaagaga uauucaacuc ugacaaacaa uuaguagcua uauuuaaacu aucagugggu      720

ucaaguguca gucuaaacga cuuauucgac acagacgaau auaagaaagg agagguggaa      780

aaagaaaaaa uaaguuuccg agaacaaauc uacgaagacg acaaaccaau auacuauuca      840

auauuggggg aaaagauaga acuucuagau auagcaaaau cuuuuuacga uuucaugguu      900

cuaaacaaca ucuuggcaga cagccaguau auaagugagg caaaaguaaa acuauacgaa      960

gaacacaaga aagacuugaa aaaucucaag uacaucauuc gcaaauauaa uaaaggaaau     1020

uaugacaaac ucuuuaaaga uaaaaaugaa aauaacuaca gcgcauauau aggauugaac     1080

aaagaaaaag gaaaaaaaga gguuguagaa aaaaguagau uaaagauaga ugacuuaaua     1140

aaagucauca aaggauaccu acccaaacca gagaggauag aagaaaaaga caaaacaguc     1200

uucaaugaga uauuaucaaa aauagaacua aaaacaauau ugcccaaaca aaggauauca     1260

gauaacggga cuuuaccaua ucagauccac gagguugaac uagagaaaau acuagaaaac     1320

caaucaaaau auuaugauuu ccugaacuac gaagaaaaug gcguaagcac caaagacaaa     1380

cuacuaaaaa cauuuaaauu ccgaauacca uacuacguag gcccacuuaa cagcuaccau     1440

aaagacaaag gaggaaacag uuggaucgug aggaaagaag agggaaaaau acuaccaugg     1500

aauuuugagc aaaagguuga caucgaaaaa ucagcagaag aauucauaaa aagaaugaca     1560

aacaaaugca cauaucuuaa uggagaagau guaauuccaa aagauacauu ccuauauagc     1620

gaauauguaa uccuaaacga acucaauaaa guacaaguaa augacgaauu ucuaaaugaa     1680

gagaauaagc gcaaaauaau agacgaacua uuuaaagaaa auaaaaaagu auccgaaaaa     1740

aaauuuaaag aauaucuccu ugucaaacaa auaguagaug gaacaaucga auugaagggu     1800

guuaaagaca gcuucaacuc aaacuauaua agcuacauuc guuucaaaga cauauuuggu     1860

gaaaaauuga accuagacau uuauaaagaa auaagugaaa agucaaucuu guggaagugc     1920

cucuacggag acgacaaaaa aaucuucgaa aaaaaaauca aaaacgaaua uggagacauc     1980

cugacaaaag acgagaucaa aaagauaaac acauucaaau ucaauaauug gggccgacuc     2040

agcgaaaaac uauuaacggg aaucgaauuc auuaaccuag aaacaggcga augcuauuca     2100

ucaguuaugg acgcucuacg gcgcacaaau uacaaccuaa uggagcuauu gagcuccaaa     2160

uucacguuac aagaaaguau aaauaacgag aauaaggaaa ugaaugaagc aagcuaucga     2220

gaucuaauug aggaaucaua cguaaguccu agucuaaagc gagcaaucuu ucagacucua     2280

aaaaucuaug aagaaauacg gaaaauaaca ggcagaguac caaaaaaagu cuucauugaa     2340

auggcaagag gcggagacga aagcaugaaa aauaaaaaaa uacccgccag gcaagagcaa     2400

cucaaaaaau uguaugacuc cugcggaaac gacaucgcaa acuucaguau agacauaaaa     2460

gagaugaaaa acucacuaau aucuuacgau aauaauucau uaaggcaaaa aaaacuauac     2520

cuauacuauu uacaauuugg aaaaugcaug uacacaggcc gagaaauaga ccucgacagg     2580

cuacuacaaa auaaugacac guaugauauc gaccauauuu acccgcgguc caaaguaauc     2640

aaggacgacu cauucgacaa ucuuguacuc gugcuaaaaa acgaaaaugc agagaaaagu     2700

aacgaauauc caguaaaaaa agaaauccaa gaaaaaauga aaucauuuug gagauuucua     2760

aaagaaaaga acuuuauauc agacgaaaaa uacaaaagau ugacagguaa agacgauuuu     2820

gaacuacgug gauuuauggc aagacaauua gucaacgucc gacaaaccac aaaagagguc     2880

gguaaaauac uccaacaaau agaaccagaa auaaaaauag uauauaguaa agcggagaua     2940

gcgucaagcu ucagggaaau guuugacuuu aucaagguac gagaauuaaa ugacacacac     3000

cacgccaaag acgcuuacuu aaauauuguc gcaggcaaug uauauaauac aaaauucacg     3060

gaaaaaccau aucgcuacuu acaagaaaua aaagaaaacu acgacguaaa aaagauauau     3120

aacuacgaua uuaaaaaugc cugggauaaa gaaaacucac uagaaauagu aaaaaaaaac     3180

auggaaaaaa auaccguaaa cauuacacga uucauaaaag aagaaaaagg acaacuauuu     3240

gaccuuaacc caaucaaaaa aggagaaaca ucaaacgaaa uaauaagcau aaaaccuaag     3300

gucuauaacg gcaaagacga uaaauuaaau gaaaaauaug gauacuauaa aagcuuaaac     3360

ccugcauauu ucuuauacgu agaacacaaa gaaaagaaca aacgaauaaa gagcuucgaa     3420

agaguuaauc uaguagacgu uaacaauaua aaagaugaaa aaagccuagu caaauaucua     3480

aucgaaaaca aaaaauuagu agaaccacgc guaaucaaaa aaguauauaa aagacaagua     3540

auucuaauaa accacuaccc auacaguaua guaacauuag auagcaauaa acuaauggac     3600

uucgagaauu uaaaaccauu auuccuagaa aacaaauaug aaaagauacu uaaaaaugua     3660

auaaaauuuc ucgaagacaa ucaagguaaa uccgaagaaa acuacaaauu cauauaucua     3720

aaaaaaaaag acagauacga aaaaaaugaa acgcuagaau ccgucaaaga ucgauacaau     3780

cuagaauuua acgaaaugua cgauaaguuc cuagaaaaac ucgacagcaa agauuauaaa     3840

aauuacauga acaauaagaa auaucaggaa uuacuagaug uaaaagaaaa auucauaaag     3900

uugaaccucu ucgauaaggc auucacacua aaaucauucc uagaccuauu uaacagaaaa     3960

accauggcag acuucucuaa aguaggacua acaaaauacu uaggcaaaau ucaaaaaaua     4020

uccucgaacg uccuaucaaa aaaugaauua uacuugcuug aagaauccgu gaccggccuc     4080

uuuguaaaga aaauaaaacu uuga                                            4104


<210>  5416
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5416
augaaaaaac agaaauucag cgacuauuac cuagguuucg auauaggcac caauucagua       60

ggauggugcg uuacagaccu agacuauaau guacuacgau ucaacaaaaa ggauaugugg      120

ggaucccgau uguuugacga ggccaaaacc gccgcugaaa gaagagucca aagaaauagu      180

cguagaaggc ucaaacgacg aaaauggaga cuaaaucuuc uagaagaaau auucagcgau      240

gaaaucauga agaucgacag caacuuuuuc cgaagacuaa aagaaucauc ccuauggcuc      300

gaagauaaaa acaguaaaga aaaauucaca cuauucaaug augacaauua uaaagauuac      360

gacuucuaua aacaguaccc cacuauuuuc cacuuaagag augaacuaau caaaaacccg      420

gaaaaaaaag acaucagacu aauuuaucua gcacuacacu caauauuuaa gagcagaggc      480

cacuucuuau ucgaaggcca aaaccugaaa gagauaaaaa auuuugagac acuauauaac      540

aacuuaauca guuuuuuaga ggauaacgga aucaauaaau caauagacaa ggauaauaua      600

aaaaaacucg aaaaaauuau augcgacucg ggcaaaggcc uuaaagacaa agaaaaagaa      660

uuuaaagaaa uauucaacuc cgauaagcaa cuaguagcca uauuuaaacu uucaguaggc      720

aguagcguuu cccuaaacga cuuguuugac acagacgaau acaaaaaaga agagguagaa      780

aaagaaaaga uuaguuuuag agaacaaaua uaugaagacg acaaaccaau auacuacagc      840

auacuaggag aaaaaauaga acucuuagac auugcgaaau ccuuuuacga uuucaugguc      900

cuuaacaaua uauugagcga uagcaauuac aucucggaag caaaaguaaa gcucuacgaa      960

gagcacaaaa aagaccuaaa gaauuuaaaa uauauaauac gaaaauauaa caaagaaaac     1020

uacgacaaau uauuuaaaga caaaaaugaa aauaauuacc cagcauauau cggccucaau     1080

aaggaaaaag ggaaaaaaga aguuguagaa aaaucaggac uaaaaauaga ugauuuaaua     1140

aaaguuauaa agggcuaccu uccgaaaccc gaaaggauag aagagaaaga caaaaccauc     1200

uuuaaugaga uacuuaacaa aauagaacuu aaaaccauac uaccaaaaca acgaauaagc     1260

gauaauggca cacugcccua ucaaauccac gaaguagaac uagagaaaau acuagaaaau     1320

caaagcaaau auuaugacuu ccucaauuau gaagaaaaug gagucagcac caaagacaaa     1380

uuacuuaaaa cauucaaauu ccgaauacca uacuacgucg gaccgcuaaa uagcuaccac     1440

aaggauaaag gagggaauuc auggaucgua agaaaggaag aaggaaaaau ucucccaugg     1500

aauuuugaac aaaaaguaga cauugagaaa agcgcugaag aauuuauaaa aaggaugaca     1560

aauaaaugca cauaucuaaa cggagaagac gugauaccaa aagacagcuu ccuguacagc     1620

gaauacauaa uccuaaauga acuaaacaaa guacagguca augaugaauu ucuaaacgaa     1680

gagaacaaaa gaaagauaau cgacgaauua uuuaaagaaa acaaaaaagu aucagagaaa     1740

aaauucaaag aauaucuacu agucaaccaa auagcaaauc gaacaguaga gcuuaaaggc     1800

auaaaagaca gcuuuaacuc aaauuaugua ucauacauua aguuuaaaga cauauuuggu     1860

gaaaaauuaa aucuugacau auacaaggaa auaagcgaaa aaucaauacu uuggaaaugc     1920

cuauacggug augauaaaaa gauuuuugaa aaaaaaauaa aaaacgaaua cggugacaua     1980

uuaaauaaag augaaauaaa aaaaauaaac ucguuuaagu ucaauacuug gggccgauua     2040

ucagagaagc uauuaacagg gauagaguuu auaaaccuag aaacaggaga auguuauucc     2100

ucggucaugg aagcucugag aagaaccaac uacaauuuga uggaauuacu aagcucaaaa     2160

uuuacauugc aagaaucaau cgauaaugga aauaaagaga ugaaugaagu gagcuacagg     2220

gaccuaauug aagaaucaua cguuucacca agccucaaac gagcuauacu acaaacacuc     2280

aaaauuuacg aagaaauaaa aaaaaucacu ggcagagugc ccaaaaaagu auuuauugaa     2340

auggcacgug gaggagacga aacuaugaaa aauaagaaaa uaccagcaag acaagaacaa     2400

cuuaagaaac uauaugauuc augcggaaac gacauugcga acuuuagcau agacauaaaa     2460

aaaaugaaaa guucacuauc cucuuaugac aauaacagcc uaagacagaa aaaacuauac     2520

cuauauuauc uacaauucgg caaaugcaug uacacuggaa gagaaaucga ccucgacaga     2580

cuucugcaga acaacgacac cuacgauaua gaucauauuu acccacgauc gaaagucaua     2640

aaagaugaca gcuuugacaa ccuaguguua guacuaaaaa acgaaaacgc agaaaaaucg     2700

aacgaauacc ccguaaaaaa agaaauacaa gaaaaaauga aaucauucug gagauuccua     2760

aaagaaaaga acuucauauc cgaugaaaaa uauaaaagac uuacaggcaa agacgauuuc     2820

gaacuaagag gauuuauggc aagacaauua gugaacguaa gacaaacaac caaagaagua     2880

ggcaaaauau uacaacagau cgagcccgaa auaaaaauag uguauucaaa agcagaaauc     2940

gcuagcagcu ucagagaaau guucgauuuu auaaaaguac gcgaacuaaa cgauacccac     3000

cacgcaaaag acgcauaucu aaacauaguc gcugggaacg uuuacaauac caaauucacc     3060

gaaaaaccau auagauauuu acaagaaaua aaagaaaauu acgacgugaa aaaaauauau     3120

aacuaugaua uaaaaaaugc cugggacaaa gaaaauucac uagaaaucgu gaaaaaaaac     3180

augaaaaaaa acacagucaa uauaacacga uuuauaaaag aggaaaaagg acaguuauuc     3240

gaccuaaacc caaucaaaaa aggagaaaca aguaaugaaa ucauaaguau aaaaccaaaa     3300

cuguauaacg gcaaagaaga aaaacucaac gaaaaguaug gauacuauaa guccuuaaau     3360

ccggccuauu uccucuacgu ugaacauaaa gaaaaaaaca aacguaucaa aucauucgaa     3420

aggguaaauu uaguggacgu uaauaacaua aaagacgaaa aaucauuagu aaaauaccua     3480

auagaaaaca agaaacuagu ugaaccacgu cucauaaaaa aaguauauaa acgacaagua     3540

auacuaauca augauuaccc auauaguauu gucgcacucg auucaaauaa gcucauggac     3600

uucgaaaacc uaaaaccucu auuccuagaa aacaaauacg agaagauauu aaaaaacgua     3660

aucaaauuuc uagaagacaa ccaagguaaa agugaagaaa auuauaaauu uaucuacuug     3720

aaaaaaaaag accgguauga aaaaaaugaa accuuggaau caguaaaaga uagauauaau     3780

cuagaauuua acgaaaugua cgacaaauuc cucgaaaaac uagauaguaa agauuauaaa     3840

aacuauauua acaauaaaaa guauaaaaac cuacuagaug uaaaggaaaa auuuaucaaa     3900

cugaaucuau ucgauaaagc guuuacuuua aaaucauucc uggaucuauu caaucgaaaa     3960

acgauggcug acuucagcaa aguaggacua accaaguacu uaggaaaaau acaaaaaauc     4020

uccaguaacg uauuaucaaa gaaugagcuc uaucuccuag aagaaagcgu aacaggccuc     4080

uucguaaaaa agauaaaacu uuga                                            4104


<210>  5417
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5417
augaaaaaac aacaauucag cgacuacuac cuaggauucg acauaggaac aaacucagua       60

ggguggugcg uuacggacuu caacuacaac guuuugagau ucaacaagaa ggauaugugg      120

gggucccgau uauucgauga agcgaaaaca gcagcagaaa gacgaguuca aagaaauagc      180

agacgcaggu uaaaaaggcg aaaauggcgu uugaacuuac uagaagaaau uuucucagac      240

gaaauacuca aaauagauuc caauuucuuc agaaggcuaa aagaaucauc ccuaugguua      300

gaagauaaaa guagcaaaga aaaauuuaca cucuucaaug acgacaauua caaagacuau      360

gauuucuaua aacaauaccc gaccaucuuc caccucagaa augaauuaau uaaaaaccca      420

gaaaaaaaag auauacgacu uguuuaccug gccuugcacu caauauucaa aucaagagga      480

cauuucuuau ucgagggaca aaaucuaaag gauaucaaga auuucgaaac acuauacaac      540

aaucuaaugg cauuccuaga agacaaugac aucuacaaaa acaucgacuc aucuuauauc      600

ggcaaccuag agaacauaau augcgauuca aaaaagggac uaaaagacaa agaaaaagaa      660

uucaaagaaa ucuucaacag cgacaaacaa cuaguaggcu ucuucaaauu aucaguugga      720

ucaucaguau cacucaauga ucucuuugac acagacgaau auaaaaaagg agaaguugaa      780

aaagagaaaa uaucuuucag agaacaaauu uaugaggacg acaaacccau auauuauagu      840

auacuugggg aaaaaauuga auuccucgac auagcaaaaa guuuuuauga uuuuaugguu      900

cucaauaaua uuuuagcaga uagccaauau aucucagaag cuaaaguaaa acuguaugac      960

gaacacaaac gcgaucuaaa aaaccuaaag uacauaaucc gcaaauacaa caaagaaaac     1020

uacgauaaac uauucaaaga caaaaacgag ucaaauuacu cagcauauau aggucuaaau     1080

aaagaaaagg gaaaaaaaga agugauagag aaauccagac uaaaaauuga cgauuucgca     1140

aaaauuauaa aaggauacuu accgaaagcc gagaaaaucg augaaaaaga cagaaguaua     1200

uucaacgaaa uauuagauaa aaucgagcuu aaaaccauac uacccaaaca acgaauaagc     1260

gauaacggca cccuaccaua ucaaauacau gaggcagaac uggaaaagau auuggagaac     1320

caagccaaau acuacgacuu cuuaaaccau gaagaaaaug gaauauccac aaaagacaaa     1380

uuacuuauga cauucaaauu cagaauaccc uauuacgugg guccacuaaa cucauaccac     1440

aaaaacaaag gaggaaacag cuggauaguu cgaaaagaag aaggcaaaau ucuaccaugg     1500

aacuucgaac aaaaaguaga cauagaaaaa uccgcagagg aauucauaaa aagaaugaca     1560

aacaaaugca ccuaucucaa uggggaagau guuaucccga aagacucauu cuuauacuca     1620

gaauacauaa uauuaaauga auuaaauaaa gugcaaguua acgaugaauu cuuaaauaag     1680

gaaauuaaaa aaaaaauaau agaagauuua uucaagaaau caaaaaaaau cagcgaaaaa     1740

aacuucagag aauaucuacu aguaaaucag auaacaaaca aaacagugga gcugaaaggu     1800

guaaaagacg ccuuuaacuc aaauuaugua ucguauauca aauucaaaga uaucuucgga     1860

gacaaacuaa accuggauau cuacaaagaa aucagcgaaa aaaguauacu auggaagugc     1920

uuauauggag augacaaaaa aauuuuugaa aagaaaauua aaucaguaua cggcgacauc     1980

cuaacaaaag acgagauaaa aaaaauaaac ucguuuaaau ucaacacgug gggccgauua     2040

agcgaaaagu uguuaacaga aaucgaauuc auagauuugg aaacaggaga auguuacuca     2100

aguguaaugg acgcacuaag acguacaaac uauaauuuaa uggagcuucu uagcucaaaa     2160

uucacacucc aagagaacau agacaacgaa aacaaagaag uaucagaauu uagcuauaga     2220

gaccucgucg aagaaagcua uguuucccca ucuuuaaaaa gggcaauacu gcaaacuuua     2280

aaaauauacg aagaaaucag gaaaauaaca gguaggauac caaaaaaagu auucaucgaa     2340

auggcaagag gcggagacga aacuaugaaa aauaaaaaaa uacccgcaag acaagaacaa     2400

uugaaaaaau uauaugacuc augcggaaaa gacauaucca acuucucuau cgacaucaaa     2460

gaaaugaaaa acagcuuaaa cagcuaugau aauaauucac uaagacaaaa aaaacucuau     2520

cuauacuacc uacaauucgg caaauguaug uauacaggaa aagaaauuga cuuaaauaga     2580

cuauuacaaa acaacgacac cuaugacaua gaucauauau acccacgauc gaaaguaauu     2640

aaagacgauu ccuucgacaa ucuaguacuc guacuaaaga acgaaaaugc cgaaaaauca     2700

aaugaauauc cacuaaaaaa agaaauacaa gaaaaaauga aaaguuucug gaaguuucua     2760

aaagaaaaaa acuuuaucuc agacgaaaag uauaaacgac uaacagggaa agaugaauuu     2820

gaacuaagag gguucauggc ucgucaacuu guaaacguac gucaaacuac aaaagaagcu     2880

ggaaaaaucc uacaacagau agagcccgaa aucaaaauug uauauuccaa agcagaaaua     2940

gcaagcucau uucgagaaau guuugacuuc aucaaagucc gagaacuuaa ugauacccau     3000

cacgcgaagg augcauaccu gaacaucgua gcuggaaacg uuuacaauac aaaauucacu     3060

gagaaacccu accgauaucu acaagagaua aaggaaaacu acgauguaaa aaaaauauac     3120

aauuacgaca ucaagaacgc cugggauaag gaaaaauccc uugaaaucgu gaaaaaaaac     3180

auggaaaaaa acaccguaaa cauaacuaga uucauaaagg aagaaaaagg ccaacuauuc     3240

gaccuuaacc ccauaagaaa aggagagaca agcaaugaaa uaauagcaau aaaaccgaaa     3300

uucuacgaag gcucaaccga gaaauuaaau gaaaaauaug gauauuacaa auccuuaaau     3360

ccugcauacu uuauauaugu agagcauaaa gaaaaaaaca aaauaauaag aaguuuugaa     3420

agaguaaacu uaguagacgu aaauaagaua aaagacgaaa aaucacuaau uaaauaucuu     3480

auugaaaaca aaggguuaau agaacccaaa guaauaaaaa aaguauauaa acgccaagua     3540

auucucauaa acaacuuccc cuacagcaua guggcccuag auuccaauaa acuaauggau     3600

uucgagaacu uaaagccccu guuccuugaa agaaaauacg aaaaaauacu aaagaaugca     3660

auaaaauucc uugaagacaa ccaaggaaaa acagaagagu acuacaaauu ucucuaccug     3720

aaaaaaaaag acagaaacga aaaaaacgaa acaauagacu cagucaagga acgauauaau     3780

auugaauuca augaaaugua cgauaaguuc cuagagaaac uagacucaaa agacuauaaa     3840

aauuacauaa acaauaaaaa auacucagaa cugguuaacg uaaaagaaaa auucaucaaa     3900

cugaauuuau ucgacaaagc auucacccua aaaucuuuuc uagaccuuuu uaaucguaaa     3960

acaauggcag auuuuagcaa aguuggucuu acgaaauacu uaggaaaaau acaaaaaaua     4020

ucaucaaacg uacuuucaaa aaacgaacua caccuccuag aagaaagcgu cacaggccua     4080

uucgucaaaa aaauaaaauu auga                                            4104


<210>  5418
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5418
augaaagacu auuacuuagg guuagacaug gguuccucau caguuggaug ggcgcucucc       60

gacgaaagau auaacuuacu aagagcaaaa ggaaaagacc uauggggagu acgauuguuc      120

gaugaagcaa aaacagcugc agaaagaaga acauuuaggu cucaacguag acgacuugaa      180

agguccaaau ggagauuauc acuacuuaca ucuuuauucg aagaagaaau aucuaaaaua      240

gacgauaaau ucuuugauag acuaaaagau aguaaauucu ucauugauga uaaaucagau      300

caacaaccaa auacacuauu caacgaugua aacuucacag auaaagaaua ccacaaaagg      360

uacccaagua uauaccaucu aagaaacgca uuauuuaauc aaaacgacaa auucgacauc      420

cgccuuauau uucuagcauu aucacauaua gucaagaaaa gaggacacuu ucuuuucgau      480

ggagauaaag caggguuugg ggaagacaua gacguaaucu uagaugaauu aaaagcauac      540

uuacaagacg cauuucacac aaaacuagaa uuuacuagcg aauuuaaauc aaucaugcua      600

gauaaaaacc uaaggaagaa acaaaaagaa gaagaacuca aaaacucaau aaaaauauca      660

agugacacaa uaaauucaaa agacgggaaa aguauacuca uaaaauuauu ucuaggauac      720

gaagcuaauc uaaaacccuu cuuucucgaa uacaaagucg aaaaagacaa cgaauacaaa      780

auaaauuuuu cgaaagaaga cuaugaagaa aaacuauuag acaugacuuc aaaauucgaa      840

gaaacaacau auuuuauuga aaucgcaaag aaaauacacg auuacuuccu uuuagaagaa      900

auaaugcucg gauucaaaaa cauaagcgca ucaaaaguuu cgauuuacca aaaacacaaa      960

aaugaccuaa aaacccugaa aaacauuguc aaaaaucaaa uaaauaauuc uuacaacgcc     1020

auauucaaag auauaggacu ggauaacuac ccagcuuacg uaagcuguug gaacagcaag     1080

aaacacaaag aaaaacaaaa aggauguaca aaagacaaau uuuacgcuuu cuuaaaaacu     1140

aaacuuaaaa auauuaauaa cgcaaaagaa auccucgacu ccaucgacaa ugaugacuuu     1200

cuacccaggu uaguggacaa aaacucaauu aucccacauc aaauacaucu cgcagaauua     1260

aucaaaauaa uagacaauca aagccaauau uaccccuucc uuaaagaaau aaaagacaaa     1320

auaauaucaa ucuucaaauu ucgcauacca uuuuacgucg gaccacuaaa uaauuuccau     1380

aaagauaaag gaggaaaugc uuggaucgaa aaacuaaaag augaacgaau cuuuccaugg     1440

aacuucaacg acgucgugga cuuaaacaaa ucagcugcca acuuuauaga gaaaaugaua     1500

aaugaaugcu cauaccuaaa aggugaaaaa gugcuugcca aaaacucuuu acuauacaca     1560

aaauauucgg uauuaaacuu gauuaauaaa cuaaaaauca augguacagc aguuucagua     1620

gaaacaaaac agaaaauaua caacgaauug cuacugaaac agaaaaauau caagaaaacc     1680

gcuaucaaga aauuccuuau caacaacuca auaauagaua aaaacgacga aauaagcggc     1740

auagaugauu cacuaacaag caaucuagca gucuucauag auuuugagaa aauaaguaac     1800

cgguuaagau acgaccaaaa agaagacauu auaaaaagca ucuccuuauu cggauacgac     1860

aacgcaaugc ucaagaaaca aaucauuacc auagcucccg aucuguccga agacgaauua     1920

gaaaaacugu cuaaacuaag auauuccgga uuuggacgau uauccaaaag auuauuaaca     1980

agcauaaaac acaauggaaa aaguauuauu gaaaugcuau gggacacaaa cgauaaccua     2040

auggaacucc uuucagauag auauacuuuc aucgaacaaa uaaacaaaca caacgacgga     2100

uuuaaagaag auauuaaccu uaacuuccaa aauguagaca cucucuacgu cucaccgagu     2160

guuaaacgac aaauuuggca agcauuuaaa guaauaaacg aauuaauuag cguuaugggu     2220

agagagccaa agaaaaucuu ugucgaaaug gcccgcgaag aaggcgaaaa gaaaagaacg     2280

caagccagaa aagauaaagu auuagaacua uauaaaagua ucaaaaacaa cgaaauauca     2340

acauuccuua caaacuuuaa agacauuaaa acgaaacucg agggagaauc caacuuaaau     2400

agcaagaaac ucuaccucua cuauuugcaa cuuggaguag auauauauac aggagagaaa     2460

auacccuucg aaaaacuuuu ugaccaaaac auauacgaca uugaccacgu aauacccaga     2520

ucccuaaaga aagaugacuc auucgauaau cuaguacuaa caucuaaaac aaauaaugca     2580

aagaaaacgg augaauuucc cguuucguca gaaauucaaa guaaaaugca aaccuuuugg     2640

aaaguacuua aagacaaaga cuuaauguca gaaauaaaau acacaaaucu caccagaaaa     2700

acacauuuau cagaugacga auuaggcgac uuuauagcca gacaacucgu cgaaacucgc     2760

caaaguacua aagcacucac acaaauacuc aaaaacguau acgcaaacuc agauaucgua     2820

uacguuaaag cagggauagu cuccgaauuu cgacaacaau uugauaugcu aaaaauaaga     2880

gaacuaaacg acuuccacca ugcuaacgac gcauacuuaa auauaguuau aggaaaugua     2940

uauaacauaa aauuuacuaa aaacccgcgc aacuacguaa aagaauacua uaagaaaggc     3000

gaacacuaua aucuuaaaau agagaaaaug cuagaaaaag auauagcacg aggagguaac     3060

uuuggauggg acaagaaaca caguauagaa accguacaga aaacaaugaa gaaacaaucc     3120

ccacuuauaa cuuacagaac cuucgaaaac aaaggugaau uuuucaacca acaacccucu     3180

aaagcaucag ccaaucuucu agcccugaaa ucaagcgaca aaagauuacu agacacagug     3240

cgauacgggg gauauaaagg aacgcaauau gcacacaugu ucuuaguuga acauuucaau     3300

ggaaaaauca uaaaagaaau aaugggcgua ccaauuuacu uaaguaaaga aauaaaagac     3360

gacaaagcaa aacucuuaga auauuguaau gacguacuaa aacucaaaaa ccccaaaaua     3420

cuauucucua aaauacccua caacucacua auaguauaca acggauaucc aauucuacua     3480

aggggacuaa ccggaguaag aggagcggca caauuaaagu uagaucaaac aucaacacaa     3540

guagugaaga auauaguaaa auuauacgac uggcuuucac aaaaacaaaa agaacucucg     3600

gaccuaucca agaaaauaga auuuuuccaa gaaaacaaua agaaagauga aguaaaagcu     3660

gccaugcuag aagcagacaa uauauuguuc auacucaacg aucguuucaa aaauaccaaa     3720

auaggaaagg acauaaugaa ugacaaccac cuaaauaacu uauacgauac auucauaaac     3780

aaauuggaaa acuccauaua uaaaaacaag ccagggggua aacaagaugu auuaaugaag     3840

gagaaaagag aucuauuccu uucauuuagc auguccaaga aauguauaac auuauacaaa     3900

auaaucaaaa uauucggauc augcgaccaa gguguagacu uuagcgauau cgcagaaaag     3960

auaagcaagu caggaggaau agguggcaaa aacuuaggca aaaauacuuu ugaccuagua     4020

uuuaaagaaa auagcaccua uacgcuagua caaaguucag ugacaggacu auacgcuaag     4080

aaaacagauc uacauaaacu auga                                            4104


<210>  5419
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5419
augaaaaaac agaaauuuuc agacuacuau cuaggauuug acauaggaac aaacuccguc       60

ggauggugcg uaaccgaucu ugacuacaac guccuaagau ucaauaaaaa agacaugugg      120

ggauccagau uauucgagga agcgaaaaca gcugcggaac ggagagucca acgcaauagu      180

cgaagacgau uaaaaagaag aaaauggaga cuaaauuuac uagaagagau uuucucaaac      240

gaaauacuca aaauagauuc caacuucuuc aggagacuaa aggaaucauc guuauggcuu      300

gaagauaaau caagcaaaga aaaauuuaca cuauuuaaug augacaauua uaaagauuau      360

gacuucuaua aacaauaccc aacaaucuuc caccuuagaa acgaacuaau aaaaaacccc      420

gaaaagaaag acauaagacu aguguacuua gcaauacauu caaucuuuaa aagccgagga      480

cacuuuuugu ucgaagguca aaaucuaaaa gagauaaaaa auuucgaaac uuuauauaau      540

aaccuaaucg cauuccucga agacaacggc aucaacaaaa uaauagacaa aaacaacauc      600

gaaaaacuug aaaaaaucgu augcgacuca aaaaagggau uaaaagauaa agaaaaagaa      660

uucaaagaga uauucaacag ugacaaacaa uuaguagcaa ucuucaaacu uaguguuggu      720

agcagcgucu cccuaaacga ccuauuugac acggaugaau acaaaaaagg agaaguagaa      780

aaagaaaaga ucagcuucag agaacaaaua uaugaagaug auaaaccgau auacuauuca      840

aucuuggggg aaaaaauuga auuacucgau auagcaaaaa ccuuuuacga cuucaugguu      900

uuaaacaaca uacucgcuga uucacaauau auaucagaag caaaagucaa acuauacgaa      960

gaacauaaaa aagacuuaaa aaaccuaaaa uacauaauca gaaaauacaa caaagguaau     1020

uacgacaagc uauuuaaaga uaaaaacgaa aauaacuaca gugcauacau aggacuaaac     1080

aaagaaaaau caaaaaagga gguaauagaa aaaagcagac ucaaaauaga ugaucuaauc     1140

aaaaacauca aaggcuaccu uccgaaaguc gaagaaauug aagaaaaaga uaaggccauc     1200

uuuaauaaaa uacuaaacaa aaucgaacua aaaaccauac ucccaaaaca gcgaaucucu     1260

gacaauggca cucuaccaua ucaaauucac gaagccgaac ucgaaaaaau acuagaaaau     1320

cagucgaaau acuaugauuu ccuaaauuac gaagaaaaug gaauaaucac aaaagacaaa     1380

cuacucauga cauucaaauu caggauacca uauuacgucg gaccgcugaa cagcuaucau     1440

aaagacaaag gaggaaacuc auggauagua agaaaagaag aaggaaagau acuuccaugg     1500

aauuuugaac aaaaaguaga cauagaaaag ucugcugaag aauuuaucaa aaggaugacu     1560

aacaaaugua cauaucuaaa cggagaagac guaauuccaa aagacacauu uuuauacuca     1620

gaauacguaa uacuuaauga acuaaauaaa guccaaguaa augacgaauu ccuaaaugaa     1680

gagaacaagc gaaaaaucau agaugaacua uucaaagaaa auaaaaaagu aucagaaaaa     1740

aaauuuaaag aauaccuccu agucaaacag auuguggaug gcacuaucga acuuaaggga     1800

gugaaagauu ccuuuaauuc gaauuacauu ucuuauauaa gauucaaaga caucuucgga     1860

gaaaaacuga aucucgauau auacaaagaa auaucagaga aaucaauccu uuggaaaugu     1920

cuauacggcg acgacaaaaa gauuuuugaa aaaaaaauua agaacgagua cggcgauauu     1980

cugaccaagg acgaaauaaa aaagaucaau acguuuaagu uuaacaacug gggaaggcua     2040

ucugagaaac ucuuaacugg aaucgaauuc auaaaucuag aaacuggcga auguuauuca     2100

uccguaaugg augcacuaag acgcaccaac uauaaccuca uggaacuccu aagcucaaag     2160

uuuacuuuac aagaaucgau uaacaacgaa aacaaagaaa ugaaugaagc aaguuacagg     2220

gacuuaauug aagaaucaua uguuagcccu ucuuuaaaga gagcaauauu ccaaacguua     2280

aaaauauacg aagagauaag aaaaauuacu ggaagagucc cuaaaaaggu auuuauugaa     2340

auggcgagag gcggagauga aucuaugaaa aauaaaaaaa uaccugcccg acaagaacaa     2400

cucaaaaaac uuuacgacuc augcggaaac gacauagcga acuucagcau agacauaaag     2460

gaaaugaaga auagccuaau aaguuacgac aacaauagcu uacgccaaaa aaagcuauac     2520

cuauacuacc uccaauucgg caaauguaug uauacaggca gagaaaucga ccuagaccga     2580

cuauuacaaa acaacgacac auaugauaua gaucauauau auccuagauc gaaagugaua     2640

aaagacgaua guuuugacaa cuuaguacuu gugcuaaaaa augaaaaugc agaaaaguca     2700

aacgaguacc caguaaaaaa ggagauacaa gagaaaauga aauccuucug gagauuuuug     2760

aaagaaaaaa acuucauauc agacgaaaaa uacaaaagac uuaccggaaa agaugacuuc     2820

gaacuccgag gauuuauggc acggcaacug guuaacgucc gccaaacaac gaaggaggua     2880

ggaaaaauau uacaacaaau agaaccagaa auaaaaauag uauacaguaa agccgaaauc     2940

gcgaguagcu uccgggagau guucgacuuu auaaaaguga gagaauuaaa ugacacccac     3000

cacgcgaaag acgcauaccu gaacauagua gcuggcaaug uauacaacac aaaauuuacg     3060

gaaaaacccu auagauaccu acaagaaaua aaagaaaauu acgacguaaa aaaaauauac     3120

aacuacgaua uaaaaaaugc gugggauaaa gaaaauagcc uggagauagu caaaaaaaac     3180

auggaaaaaa acaccguaaa cauaaccaga uucauaaaag aaaaaaaagg ccaacuauuc     3240

gaucuaaacc caauaaaaaa aggagaaacu ucuaacgaaa ucauaaguau aaaaccaaaa     3300

gucuacaacg gaaaagauga caaacuuaau gaaaaauaug gauacuauaa aucacucaau     3360

ccggcauauu ucuuauaugu ugaacacaaa gaaaaaaaca aaagaauuaa guccuucgaa     3420

cguguaaacc uaguagaugu aaauaacaua aaagacgaaa aaagcuuagu uaaguaccuc     3480

aucgaaaaca aaaagcucgu agagccucgu guaaucaaaa aagucuacaa aagacaagua     3540

auccucauaa acgacuaccc auauuccauc guaacacucg auaguaauaa acugauggac     3600

uuugaaaauc ucaaaccacu auuccuagaa aauaaauacg aaaaaauccu gaaaaacguu     3660

aucaaauucu uagaagacaa ccagggcaaa ucagaagaaa acuacaaguu cauauaucua     3720

aagaaaaagg acagauauga aaaaaacgag acucuagaau cagugaaaga uagauacaac     3780

cuagaauuua augagaugua ugacaaauuc cuagagaaau uagauaguaa agacuauaaa     3840

aacuacauga acaacaaaaa auaucaagaa uuacuugaug uaaaagaaaa auuuaucaag     3900

uugaaucuau ucgacaaagc cuucacguua aagucauuuu uagaccuauu uaacagaaaa     3960

accauggcag auuucucgaa aguaggacua acaaaauauc ucgguaaaau acaaaaaaua     4020

ucaagcaacg uuuugucuaa aaacgagcua uaccuacuag aagaaucagu aacaggccua     4080

uucguuaaaa agauaaaauu auga                                            4104


<210>  5420
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5420
augaaaaaac aaaaauuuuc agacuacuac cuuggauuug acauugguac uaacucugua       60

ggauggugcg uaaccgauau gaacuauaau guacuuagau uuaacaaaaa agacaugugg      120

gguagucgcc uguucgaaga agcuaaaacu gccgcagaaa gaagaguaca aagaaacagu      180

agaagacguc uuaaacgaag aaaauggaga cucaacuuau uagaagagau auuuucuaau      240

gaaauacuaa aaauugacag uaauuucuuu agaagauuga aagaaaguuc ccucuggcuu      300

gaggauaaaa gcagcaaaga aaaauuuacu uuauucaacg augacaauua caaagauuac      360

gauuucuaca aacaauaccc aacaauauuu caccuaagaa acgaacuaau caaaaacccc      420

gaaaaaaaag auauaagauu gguauaucua gcaauccaca gcauauuuaa gucacgaggc      480

cacuuccuau ucgaagguca gaaccucaaa gaaauaaaga acuuugaaac gcuauacaau      540

aauuuaauag cguuccuaga agauaaugga auaaacaaau ccauagauaa agauaaccua      600

gaaaaacuag aaaaaauagu uugugacagc ggaaaaggac uuaaagauaa agaaaaagaa      660

uucaaagaaa uauucaacuc agacaaacag cucguggcca uauucaaacu aagcguagga      720

uuaaguguau ccuuaaacga cuuauuugau acggacgaau acaaaaaagg cgaaguagaa      780

aaagaaaaaa uaaguuuccg ugaacagauu uacgaagacg acaaacccau auacuacagu      840

auccuaggcg aaaagauaga auuacucgac auagcaaaaa gcuucuacga cuucauggua      900

cucaacaaua uucuagcuga uagccaauau auaucagaag ccaaaguaaa auuauacgaa      960

gaacauaaaa gagaccuaaa aaaucuaaaa uacauaauua gaaaauacaa cagagagaac     1020

uaugauaaac ucuuuaaaga uaaaaacgaa aguaacuacc cagcuuauau cggauuaaau     1080

aaagaaaaau ccaaaaaaga aguaaucgag aaaagcaaau uaaaaaucga cgacuuaauc     1140

aaaagcauua aaggguaccu accaaaaguu gaagagauag aggaaaaaga uaaggcaauc     1200

uucaacgaaa ucuuaaauaa gauagagcug aaaauuauau uaccuaaaca aagaauaucu     1260

gacaacggca ccuugccgua ucaaauacac gaagcagaac uagaaaaaau acuggaaaau     1320

caaagcaaau acuacgauuu cuuaaauuau gaagaaaacg guauuauaac caaagauaaa     1380

cuccuaauga cauuuaaauu ucgaauacca uauuacguug gcccuuuaaa cucauaucac     1440

aaagacaaag gaggaaacuc auggauugua agaaaagaag aagguaaaau acuaccaugg     1500

aacuucgagc aaaaaguaga caucgaaaaa ucagcugaag aauucauaaa aagaaugacu     1560

aauaaaugua cauaucucaa cggagaagau gucauaccaa aagacagcuu ucuguacucu     1620

gaauauauaa uauuaaauga auuaaacaaa guacaaguca acgaugaauu ucucaaugaa     1680

gaaaacaaaa gaaaaauaau agaagagcua uuuaaaggua acaaaaaggu aucagagaaa     1740

aaauuuaaag aauaccuauu agugaaucaa auaacuacag gaaccauaga auuaaaaggc     1800

aucaaagauu cauucaauag uaacuauauc agcuacauaa aauucaaaga cauauucgga     1860

gaaaaacuaa aucuugauau auacaaagaa auauccgaaa agaguauuuu guggaaaugu     1920

uuauauggag augauaaaaa aauauuugaa aaaaaaauaa aaaacgaaua uggagacauu     1980

uuaacaaaag acgagauaaa aaaaauuaau acguuuaagu ucaacaacug gggaaggcua     2040

uccgaaaaac uauuaaccgg cauagaauuu aucaaucuug aaacagguga auguuauuca     2100

ucaguuaugg aagcucuaag acguacaaau uauaauuuga uggaacuguu aagcucgaag     2160

uuuacacuac aagaaaacau agacaaugaa aacaaagaca ucucccaauu cagcuacaga     2220

gaucucguag aagagucuua cgucucaccu agccuaaaac gugcaauacu ucaaacacua     2280

aagauauacg aagaaauaaa aaaaaucacg ggccguguac caaaaaaagu guuuauagaa     2340

auggcaagag gaggggacga aagcaugaaa aacaaaaaaa ucccagcaag acaggaacaa     2400

cugaaaaaac uauacgauuc augcgguaac gacauagcua auuucagcau cgacauaaaa     2460

gaaaugaaau caucacuauc uuuauacgau aauaauucau uacgucagaa aaaacucuac     2520

uuauacuauc ugcaauucgg gaaaugcaug uacacaggga gagaaaucga ccucgacaga     2580

cuuuuacaaa auaacgacac uuacgauauc gaucacauau acccaagauc gaaaguaaua     2640

aaagacgacu cguuugacaa cuuaguauua guucuaaaaa acgagaacgc cgagaaaucc     2700

aacgaauacc cagucaaaaa agagauccaa gaaaaaauga aaucguucug gagauuccuu     2760

aaagagaaaa acuucauaag ugaugaaaaa uacaagagac uuaccggcaa agacgauuuc     2820

gaauuacgag gcuuuauggc gcggcaauua gucaacguaa gacaaacaac aaaagaagua     2880

gggaaaauau uacaacaaau agaaccagaa auuaaaaucg uauacucaaa agcggaaaua     2940

gcaaguucau uucgcgaaau guucgacuuc auuaaaguaa gagaauuaaa cgauacacau     3000

cacgcaaaag acgccuauuu aaacauugua gcaggcaaug ucuacaauac caaguucacg     3060

gaaaaaccau acagauauuu acaagaaaua aaagagaacu acgacgugaa aaaaauauac     3120

aguuacgaca uaaaaaaugc augggauaaa gaaaauucac ucgaaauagu aaaaaaaaau     3180

auggagaaaa auacagugaa uauaacacgc uuuauaaagg aagaaaaggg acagcuguuc     3240

gaucuaaacc caaucaaaaa gggagaaaca ucaaaugaaa uaauauccau uaaaccgaag     3300

guauacaacg gaaaagacga uaaacuaaac gaaaaauaug gcuauuauaa aaguuugaau     3360

ccggcauauu uuuuauacgu agaacauaaa gaaaaaaaua aaagaaucaa aucuuucgag     3420

agaguaaauc uaguagacgu aaauaacauc aaagaugaaa aaagucuaau caaauaucua     3480

gucgagaaua aaaaauuagu agaaccccgc guaaucaaga aaguauauaa acgucaagua     3540

auuuuaauaa augacuaucc uuacucaauc gucgcacucg auagcaauaa auuaauggau     3600

uucgaaaauc uaaaaccacu auuccuagaa aggaaauacg aaaaaauacu gaaaaauguu     3660

auaaaauuuc uagaagauaa ucaaggcaaa uccgaagaaa auuauaaauu uauuuacuua     3720

aaaaaaaaag acagauauga aaaaaaugaa acucuagaga gcguaagaga aagguauaac     3780

auagaauuua augagaugua cgauaaauuc cuugagaaac uaaacucaaa agauuauaaa     3840

aacuauauca acaauaaaaa auaucaagac cuauuggaug ucaaagaaaa guuuauaaaa     3900

cucaaccuau uugacaaagc uuuuacacua aaauccuucc uagauuuauu uaaccguaaa     3960

acaauggcgg auuuuucuaa agucggauua acuaaauacc uaggaaagau ccaaaaaaua     4020

uccaguaacg uauuaagcaa aaacgagcua uaccuacuug aagagucggu gaccggauua     4080

uuuguuaaaa aaauugaauu auga                                            4104


<210>  5421
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5421
auggucaaaa agaaauacgc aauaggcauc gacaucggaa cgaacuccgu cggaugguca       60

guagugaccg augauuacaa agucccgucg aagaaaauga aaguuuuugg aaacacagag      120

aaaagauaca ucaagaaaaa ucuacuagga acucuucuau uugacgaagg caauacagcg      180

gaaaaccgcc gacucaaacg aaccgcuaga agacgcuaca caagacgacg uaaccgcaua      240

cuauauuuac aagaaaucuu ugcagaagaa auaaacaaaa uugaugacag cuucuuucaa      300

cgucuagacg auaguuuccu aauaguugaa gacaaacaag gcagcaaaca cccuauuuuc      360

gguacacuac aagaagaaaa agaguaccau aaacaauuuc ccacuauaua ccaccuaaga      420

aaacaauugg cagacucauc acaaaaagcu gacauaagac uaauuuaucu agcacuagcu      480

cacauuauaa aauaucgugg acacuucuua uuugaaggcg accucaaaag ugaaaauaaa      540

gauguucaac aucuuuuuaa cgacuucgua gaaauguucg acaaaacagu agaaggaucc      600

uacuuaucag aaaacuuacc caauguagca gaugugcuag uagaaaaagu cucaaaaagc      660

agacgccucg aaaacauacu ccacuauuuu cccaacgaga agaaaaacgg ucucuucggu      720

aacuuuuuaa cucuugcauu aggcuuacaa ccaaacuuca aaaccaauuu cgaauuagca      780

gaagacgcca aaauacaauu uucuaaagaa acauaugaag aagaccucga agaacuucua      840

ggaaaaaucg gagacgauua cgcagaccua uucauagcaa caaaaucacu auaugauggc      900

aucuuacucg caggaauauu aucaaccaca gacucaacca caaaagcacc acucuccuca      960

uccaugguga auagauauga agaacaucaa aaagaccuag cgcucuugaa aaacuucauu     1020

caccaaaacu uaagcgacag cuauaaagaa gucuuuaacg auaaacugaa agaugguuau     1080

gccggauaca ucgaagguaa aacaacacaa gaaaauuucu acagauuuau uaagaaagcc     1140

auagagaaaa uagaaggcag caauuacuuc aucgacaaaa uagacaggga agauuucuua     1200

aggaaacaac gcacauuuga uaaugggagu auaccacacc agauacaucu acaagaaaug     1260

cacgcaauaa uacgaagaca agcagaauuu uauccauucc uaguagaaaa ucaagacaaa     1320

auagagaaaa ucuuaacauu uagaauacca uacuacguug gcccucucgc ucgaggaaaa     1380

uccgaguucg cuuggcucaa uagaaaaucc gaugaaaaga uacguccaug gaacuucgac     1440

gaaauggucg auaaagaaac uucagcugaa aacuucauaa ccagaaugac aaauuacgac     1500

caguauuuac ccgaccaaaa ggucuuacca aaacacagcu uacuguacga aaaauucgcc     1560

guuuauaacg aacuaacaaa agugcgauac guaacagaac aggguaaaag uuucuucuuu     1620

gacgcaaaca ugaaacaaga aauauucgac ggcguauuca aaguauacag aaagguaaca     1680

aaagaaaaac uuauggauuu ccuaggcaaa gaauuugaug aauuuagaau cguagaccuc     1740

cucggcuuag auaaagacaa caaaucauuc aacgcaucac ucggcacuua ccacgaccuu     1800

aagaaaauag uuagcaagga ucuacucgac aauccagaaa augaagauau auuagaaaac     1860

gucguacuua cacugacauu auucgaagac agagaaauga uaagaaagcg acuugaaaaa     1920

uacaaagacg uauuaacaga agaacaacgu aagaaauuag aaagacgaca uuacacugga     1980

uggggaagau ugucagcgaa acuuauaaac ggcauucgcg auaaaguuac caggaaaacc     2040

aucuuagauu accuuauaga ugacgguaca uccaaccgaa auuucaugca acuaauaaau     2100

gaugacacac ucucauucgu ugacgaaaua agacucgcac aagguucagg cgaagcagaa     2160

gacuaccgug cagaagugca aaacuuagcu ggcucucccg caauaaagaa aggcauacuu     2220

caaucacuca aaaucguaga ugaacuaauu gaaguaaugg gauacgaccc agaacauaua     2280

guagucgaaa uggcacgaga aaaucaguuc acaaaucaag guagacggaa cagccaacaa     2340

cgauauaaga aaaucgaaaa ugcaaucaaa aauuuagauu cuaauuuaaa uagcaaaauu     2400

cucaaagaau acccuacaaa uaaccaggcc cuacaaaacg acagacuauu ccucuacuac     2460

cuacaaaacg gaaaagacau guacaccgac gaagaacuug acauagacca acucagucaa     2520

uacgauaucg aucacaucau accucaagca uucauaaaag acgacucacu cgauaauaag     2580

guacucacua aaagugccaa aaaccgcgga aaaucagacg acgucccguc guuagaaauu     2640

guacauaaga agaaaaauuu cuggaaacaa uuacuagauu cgcaauuaau uagucaaaga     2700

aaguucgaua auuuaacaaa agccgaaaga ggaggacuaa caaacgaaga uaaagcacgc     2760

uucauacaac ggcaguuagu agaaacaaga caaauaacca aacacgucgc caggauucua     2820

gacacucgau ucaauacuaa acuggacgaa gcaggaaacc gaauaagaga uccaaaggua     2880

aauaucauua cuuuaaaguc aaacuuaguu ucacaguuca gaaaagauua ccaacuauau     2940

aaagugagag aaauaaacaa uuaucaucau gcacacgacg cauauuuaaa ugcugucgua     3000

gcgacagcau uacuaaagaa auauccgcaa cuugcuccug aguucgucua cggcgauuac     3060

cccaaauaca auaguuacaa aucuagaaaa uccgcaacag aaaagguacu auuuuacagc     3120

aacaucauga acuucuuucg acggguacuc guauauucua aaacaggcga agugcguaua     3180

cgaccgguca uagaagucaa caaagaaaca ggugaaauag ucugggauaa aaagagcgac     3240

uucagaacug uacgaaaagu acuaucauau ccccaaguua acguuguuaa gaaagucgaa     3300

augcaaacag gaggcuuuag caaagaauca auucuacaac acggugauuc agacaaacuu     3360

auuccgcgaa aaacagagaa auucuaccuc gauacaaaga aauacggagg auucgacagu     3420

ccaacgauag cauacucagu ucuacuaaua gcagacauag aaaaaggaaa agccaagaaa     3480

uuaaaacggg uaaaagaacu gauugguaua acuauaaugg aaaggauggc guuugagaaa     3540

aauccaauag aauuucuaga acauaaaggu uacaaaaaca uucuagagaa aaacaucaua     3600

aaacuuccca aauacagccu cuucgaauua gaaaauggcc guagacgguu acucgcauca     3660

gcuaaagaac uccaaaaagg uaaugaaaug auacuucccc cucacuuagu aacccucuug     3720

uaccacucuu caaauaucca caaaauuacu gaacccaucc auuuaaauua cgucaauaaa     3780

aacaaacacg aauuuaaaga auuacuaaga cauaucagcg acuucucaac acgauauaua     3840

cuagcacaag auagacugag uaaaauugaa gaauuguaug auaaaaacga cggugacgau     3900

auauccgacu uaacuucauc cuuuguaaac cuccucaccu uuacugcaau uggugccccg     3960

gcggccuuca aauuccuagg uagugugaua gauagaaaaa gauauacauc cauagcugag     4020

auauuagaag caacucuaau acaucaaagc guaacaggcu uauacgaaac acgcauagac     4080

cuuucaaaau uagguggaga cuga                                            4104


<210>  5422
<211>  4104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5422
auggucaaaa aaaaauaugc aauagggaua gauaucggua caaacagugu cggauggucc       60

guaguaacag acgauuacaa aguaccauca aaaaaaauga aaguuuucgg uaauaccgaa      120

aaaagauaua ucaaaaaaaa ccuuuugggc acacuccuau ucgacgaagg caacacugca      180

gagaaccgca gacucaaacg cacagcgcgc agaagauaca cuagaagaag aaaccgcaua      240

cucuaucugc aagaaauauu cgcagaagaa auaaauaaaa uugacgauuc cuucuuucaa      300

agguuggacg acucuuuccu gauaguagag gauaaacaag gcucaaaaca cccaauauuc      360

gguacauugc aagaagaaaa agaauaucac aaacaauucc caacaauaua ucaccuuaga      420

aaacaacucg ccgacagcag ccaaaaagca gacauaagac uaauauaucu ugcucuugcg      480

cauauuauaa aauaucgggg ccacuuucua uucgagggag aucuaaaaag cgaaaacaaa      540

gauguccaac aucuauuuaa cgauuucgug gaaauguucg acaaaacagu ugaaggaagu      600

uaucuuagcg aaaaccugcc aaaugucgcu gacguacuag uggagaaagu cucaaaaucu      660

cguaggcucg aaaauauauu acauuauuuu ccgaaugaaa aaaaaaacgg auuauucgga      720

aacuuccucg cacuugcacu cggacuucaa cccaauuuca aaacaaauuu cgaacuagcc      780

gaagacgcua aaauucaauu uucgaaagag accuacgaag aagaccuaga agaauuacua      840

gggaaaaucg gagaugacua cgcagauuua uuuaucgcua caaaaucacu auacgauggc      900

auucugcuag ccgguauucu gucaacuacc gacucuacaa cuaaagcacc ccuaagcagu      960

agcaugguaa auagauacga agaacaccaa aaagaccuag cgcuucuaaa gaauuucauu     1020

caucaaaauc ucagcgacuc uuacaaagaa guguucaaug auaaacuaaa agauggauau     1080

gcuggguaua ucgaaggaaa aacuacacag gaaaacuuuu auagauuuau aaaaaaagcg     1140

auagaaaaaa uugaaggcag uaauuauuuc aucgauaaaa uugauagaga agacuuccuc     1200

cgaaaacaac gcacguuuga caacggaagu auaccgcacc aaauccaucu ccaagaaaug     1260

caugcaauaa uucgaagaca agcagaauuc uaccccuuuu uaguagaaaa ccaagacaag     1320

auagaaaaaa uacucacauu ccgaauaccu uacuauguag gcccacuagc aagaggaaaa     1380

ucagaauucg ccuggcuuaa cagaaaaucg gacgaaaaga uuagaccaug gaauuucgac     1440

gaaaugguag auaaagaaac cagugcagaa aauuuuauaa cccggaugac aaacuaugac     1500

caauaccuac ccgaucaaaa aguauuacca aaacacagcc uauuauacga aaaauucgca     1560

gucuacaaug aauuaaccaa aguccgcuau gugacagaac aaggaaaauc guuuuuuuuu     1620

gacgcaaaua ugaaacaaga aauuuuugac gguguauuua aaguauacag gaaagucaca     1680

aaagaaaaau uaauggacuu ccucggaaaa gaauuugacg aauuucguau cguagaucua     1740

uuggggcuag acaaagacaa caaaucauuc aacgcguccc uaggcacgua ccacgauuua     1800

aaaaaaauag uaucaaaaga uuuacuagac aacccggaaa acgaagauau ucuagaaaau     1860

guuguccuca cauuaacccu auuugaagac cgagaaauga uaagaaaaag acucgaaaaa     1920

uauaaagaug uauuaacaga ggaacagaga aaaaaauuag aaaggagaca uuacacagga     1980

uggggucgau uaucagccaa auuaauaaau ggaauaagag auaaaguuac aagaaagaca     2040

auucucgacu acuuaauaga ugauggaaca aguaauagaa acuucaugca guuaauuaac     2100

gaugauacac uaucauucgu ugacgaaaua cgacuagccc agggaucagg agaagcagaa     2160

gacuacagag cugaaguaca aaaccuagca ggcagucccg ccauaaagaa gggcauacuc     2220

caaucacuca aaauaguaga cgaacuaauu gaagucaugg gauacgaucc agagcauaua     2280

guaguagaaa uggccagaga aaaccaauuu acuaaccagg gaagacggaa cucgcaacaa     2340

agauacaaaa aaaucgaaaa cgcuauaaag aaucuugaca gcaaucuaaa uagcaaaaua     2400

uuaaaagaau acccuacaaa uaaucaggcu cuacaaaacg aucgacuauu ccuuuacuac     2460

cuccaaaacg gaaaagacau guacaccgau gaggaacuag auauugauca guugucucaa     2520

uacgauauag accacauaau accacaagca uucaucaaag augauucccu agacaacaaa     2580

gucuuaacca aaucugcaaa aaauagaggu aaaucagacg acguuccuuc acuagaaaua     2640

guacacaaaa agaaaaauuu cuggaagcaa cuacuagauu cacaacucau cagucaacga     2700

aaauuugaca accuuacaaa agcagaacga ggaggucuca caaacgaaga caaagccaga     2760

uucauacaac gacaacuugu cgaaaccaga caaauaacaa agcacguagc aagaauauua     2820

gacacaagau uuaacacaaa gcuagacgaa gcagguaaua gaauaagaga cccaaaagua     2880

aauauaauaa cacugaagag caaucuagua ucacaauucc gaaaagauua ccaacuuuau     2940

aaaguaagag aaauaaauaa cuaccaccau gcucaugaug cauaccugaa ugcaguaguu     3000

gcaacggccc uacuaaaaaa auacccacag cucgcuccag aguucguaua cggugacuac     3060

ccaaaguaca auucauacaa aagcagaaaa ucagcaacug aaaagguacu auucuacagc     3120

aacaucauga acuucuuucg acgcguauug gucuauucaa aaacaggaga gguacgaauu     3180

agaccaguca ucgaagugaa uaaggaaacg ggagaaauug uaugggauaa aaaaagugac     3240

uucaggaccg uacgaaaagu acuaagcuau ccccaaguaa auguaguaaa aaagguugaa     3300

augcaaacug gcggauuuuc aaaagaguca auauugcaac auggagauuc agacaaacua     3360

auccccagaa aaacagaaaa auucuaccuu gacacaaaaa aauauggugg cuuugacucg     3420

ccaacuaucg cguauucagu ucuuuuaaua gcagacauag aaaaaggaaa agcaaaaaag     3480

cuaaaaagag ucaaagaauu aauagguaua acaauaaugg aaagaauggc auucgaaaaa     3540

aauccaauag aauuccucga acauaaagga uacaaaaaca uacuagaaaa aaacauaauu     3600

aaguuaccua aauauucacu auuugaauua gaaaauggca gacgacgucu acucgcgucc     3660

gcgaaagaac uacaaaaagg caaugaaaug auccuacccc cccaccuagu aaccuuacua     3720

uaucacucga guaacauaca caaaauaaca gaaccaaucc accuaaacua uguaaauaaa     3780

aauaagcacg aauuuaaaga acugcuaagg cacauaucag auuucucuac aagguauauc     3840

cuagcccaag accgacuuag caaaauagaa gaauuauacg acaaaaacga uggagacgac     3900

auaucagacc uaaccucguc auucgucaau cuucugaccu ucacagcaau aggcgcacca     3960

gcagccuuua aguuucucgg uagcguuaua gaccgaaaac gauacaccuc aauagcagaa     4020

auacucgaag caacauugau ucaccaauca guaacgggau uauacgagac aagaaucgau     4080

cuaucaaaac uaggcgggga uuga                                            4104


<210>  5423
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5423
auggacaaga aauauucaau aggccuagau auagguacau cauccauagg cuuuguugga       60

guugaugaga acggauggcc acuuaauaua aaaggaaaac gagcuauagg agcacaccug      120

uuucgagaag gugaagcugc ugcagaaaca cgcggauuca gaacaacccg ccgcagauac      180

aacaggcgaa aauggcgauu aagauuacua agagaaauau ucgacgaacc uauaucccgu      240

guagauccca cauucuucgc cagaaugaaa gaauccucag uaagucccaa agacccaaac      300

uacgauggcc uugccaaaga aaucuugauc ccaagagaca aagaugguaa aaucuuauac      360

ccuacaauau accaccuucg ucaugcauua auguacgaag accaccaguu cgacaucaga      420

gagauauacu uggccauaca ccauauuguu aaauacagag gccauuuccu acuagacggu      480

gccgcuaaaa auuuugaguc uucaaaucuc gacuuaaaaa gcgacaucca cgaaaucaau      540

gcaauucuag uaggaauauu cggcaacgaa cagguccagu ucaacgacca guacguagaa      600

aaauuaaaag agguacuauu agacucuaca caaucucguu ccgaccgcca aaaagcagca      660

gcaaaagucc uacuaccaac uaaugauuua gauaaagaca ccacaaaaau acuaaaaacu      720

gugagcacag aaauaacaaa agcuauuaca ggcuuaaaag cuaaauucuu ugucauaaca      780

ggaaacgaca uagcaaaaga ccaacaaaaa gauuggacau ucucaauaga uacuuuagau      840

gagaaaaugu cagucauaga accaucauuu ucagaaaaga auuucucuuu aauaacaaua      900

cuccaaaaau uauauucauc gguuacccuu gcugguauag uacccaaugg acucucccuc      960

agugaagcaa ugauucaaaa auacgacaga cacgcaucag accuaguuuu acuuaagaaa     1020

uacugggaca cgaaacccaa aguuuuaaaa caccaacucc aaaacacaua ugcagaauac     1080

aucgaaaucc aucaaguagu agacgaauuu uacucagauu uaucaaaauu aaucaaaaac     1140

gacgacgaca cauuagccca agaaauaaag ucgaaaauca caaucgguaa uuuccuaccc     1200

aaacaaagaa caacagaaaa uggaguaauc ccauaucaac uucaacaaag agaauuagac     1260

acaauaauca aaaaccaaca agaauauuau ccuuggcuag cugaagaaaa cccaguggcc     1320

gaucaucaga uugcuuuccc auauaaacua gaugaauuag uaggauuuag aauaccauau     1380

uacgucggcc caaugauagc uaaagaagau accagcaaca guaagaacuc uaaauucgca     1440

uggaugauuc gaaaacaaag cggagaaguc acaccuugga acuuugacga aaagauagau     1500

agaacugcuu ccgcaacagc uuucaucgau cgcaugacaa ccacagacac auaucuaauc     1560

ggugaagacg uccuaccaaa acaaagucua auuuaccaac gauucgaagu acuuaaugaa     1620

uuaaauaaaa uaucuaucga cggacaaccu auaaccagaa aacaaaaaca aucuguauau     1680

acucacguau ucgaaaagaa gaaaacaguu caaguuaaag auguacaaaa auuucuaguc     1740

ucaucuggug aaguaauguc uuuaccgaaa auagguggac uugcaaacga aaaacaauuc     1800

aacagcuccc uauccacacu auaugacuau aauaaaauaa uaccugaugc ucuagaagau     1860

agaaauaaag ucgaugauau cgagaaaauc auccucuggu caacaauauu ugaagacaau     1920

agcauauuug uugaaaaacu ugacucaaua gacuggcuca ccagcaguca acgcaaaagg     1980

uuagcacaaa aacgcuaucg gggaugggga cgauuguccc uucgauugcu uacaacauuc     2040

aaaaacaaag auggauacuc caucuuagau gaacuaugga acucuaauca caauuuuaug     2100

gaaauucaag cacagaguga cuuuuccgaa caaauagcua cugcuaacaa uaauaaccua     2160

ucucaaguuc uacccgcuaa augggaaccc ggcaggaaaa auuuagcuag cuuggacgag     2220

uucaucucca aucuuuauac aagcccacaa aauaagaaag cacuccgaaa agcaauuaga     2280

guaguccagg acaugaugac agcagcugga cauaugccag agaaaauauu cauagaaguc     2340

gccagagaua cuuccaacuc acaucaacaa acaauugcac gacaacggca auugcaacaa     2400

uuauaccaau cagaagcaaa agaaguaaua gauuccuccg uacguuccga acuagaugau     2460

auacuuaaaa accgagcuaa uauaaaagac cagcucuuuc uauauuuuca acaaggaggu     2520

agagauuugu auaccggaaa agcaaucaac auagacaaau uaagucuaua ccacauagau     2580

cauauucuac cacaaagucu gguuaaagac gacucccuag acaaucgagu acuuguauca     2640

caaaagacaa augaagacaa aggaguuacg uuugucucag auaaauacau gaauaacaug     2700

cucccuacau ggaaacguug gcacgaaaug ggacuuaucu cagcaagaaa acugaaaaac     2760

cuaacaauga gaccugacga aauagacaaa uaugcacccg guuuuauaca aagacaauua     2820

guugaaacca gacaaguaac uaaacucgua auagaacuac uagacgcgcu auuagggaac     2880

ucucaaacag aaauuauaac aauaaaggcu ucauuaucac aucaauuucg ccaacaauuu     2940

gacuuaguca aaauacgaga aguaaacgac aaccaccaug ccuucgacgc auuccuagca     3000

gcuagaaucg gaacauacuu auacaaacgc uacccaaaac uuaggccaua uuuuguauac     3060

ggucaauaua ugcaaaacca caaaaaccua aaaagcauua accuuaucaa aacccuggau     3120

ucagacaaag cuauaguaga ugaagguaca ggagaaaucc uaugggauaa gaaaucagac     3180

uuagccaaaa ugauaaaaau auauaauuau caaagacugc uaauaacaca ugaaucacgc     3240

accaguuuug gcgcaaugua caaacaaaag auuuuaaaag caacagacgc uaaacaaaac     3300

ggcggagguu uuaaacucgu accuacaaaa agaaacuggc caguagauau auacgguggu     3360

uacaccgggc aaacugcagc cuaccuaaca auaguuagag ucuacagcaa gaaaaccagc     3420

gaacuccgcg uguuacccau cuacacaaau aaaguccaac aaauugaaaa agcaaugacg     3480

caaggcgaca aagccacaau guuagccuuc aagaaaguaa ucgcuccuca auuaggaaaa     3540

gaaaacaaga aaacacauga aauaaaaaua ccagaguucg auguuguaau accuaaagug     3600

cuuuuagauc agcuaguuau ugacgacaua gcagguucac cacaccgcuu ccgauuagga     3660

acagauacau acuaccauaa uucacaacaa uuauuucugc ccuuaaguau gcaacgccaa     3720

uuaauggauc gagaaagcga ugauaaaguc uaccuugaca uuuuuaacga uguauuaaaa     3780

caaguugacu cauacuuuaa ccucuacgac gucaacggcu uuagagcugc ccuacaaaaa     3840

uccaagaaag cauuugaaca acucccaaua aacgacuuuc cuaaagaugu cccugacaac     3900

aagaagaaga aaggaaaaag auccauacug aaagaaauau ucauuggacu ccaugcaaau     3960

gcaacucuag gaaauuuaaa acaaauggga auuaaaacag acuucgguaa acuccagaaa     4020

uccaacggcu uaaggauaac agaaaacugc aaaaucauuc aucaaagucc uacagguuua     4080

uucgaaagaa uaguaccuau uaaauga                                         4107


<210>  5424
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5424
augucgagac cauacaacau agguuuagau aucggaacau cauccauagg uugguccgua       60

guugacgacc aaucaaagcu aguaucggua cgaggcaaau auggauacgg gguccgccua      120

uacgacgaag gucaaacugc ugcugaaaga agaucauuuc guacuacaag aagacgucua      180

aaacgaagaa aauggaggcu aggacuccuc cgagagauuu uugaaccuua uauaacaccu      240

guugaugaua cauucuuccu gaggcagaaa caaucgaauc uaucaccaaa ggaccaaaga      300

aaacuauauc cgcagacuag cuuauuuaac gaucgcacag accgcgcauu cuacgaugac      360

uacccuacaa uauaucaccu acguuauaaa cuaaugacag agaaaagaca auuugauauc      420

cgcgaaaucu auuuagcuau gcaccacauu guuaaauaua ggggacacuu ucucaaugaa      480

gcaccaguuu caaguuuuaa gucaagugaa aucaaucuag uagcgcacuu cgaucgucug      540

aauacaaucu ucgcugaccu auuuagugaa aguggcuucc aacucgaaac cgacaaauua      600

gcagaaguca aagcauuacu ccuggacaau caacaaagcg caucuaaucg acaaagacaa      660

gcccucucac uaaucuauac accgaguaca aauaaggcug uagaaaaaca aaacaaagcc      720

aucgcaacag aauuacuaaa agcuauucua ggauuaaaag caaaauucaa cguauuaacu      780

ggaauagaag cagaagacgu aaaagccugg acuuugacau ucaaugcuga aaauuuugau      840

gaagaaaugg uaaaacuaga aagcucuuug gaugauaaug cucaccagau aaucgaaagu      900

cuacaagaac uauauagcgg uguacuuuua gcuggaaucg uaccagaaaa ucagagucug      960

ucacaagcua ugauaacaaa auaugacgac caucaaaaac accuaaaaau gcucaaagcu     1020

guacgcgaag cucuagcacc ugaggacaga caaagacuga aacaagccua cgaucaauau     1080

gucgaugguc aagaaaacac aaaagcauau agcaaagaag auuuuuacgg ugauauuaca     1140

aaagcacuga aaaacaaccc agaccaccca auaguaagug aaaucaaaaa gcuaauagag     1200

uuagaccaau ucaugcccaa acaacgaacg aaagauaaug gcgccauacc acaccaacuc     1260

caccagcaag aauuagaccg uauaauagag aaccaacaac aauauuaccc augguuagcu     1320

gaauuaaauc cuaacucaaa acgacagacg guagcuaaau acaaacuaga ugaauuagua     1380

gccuuccgcg uucccuauua uguaggacca uuaauaaccg cugaacaaca aagacaauca     1440

ucagacgcaa aauucgccug gcugauaaga aaagcagaag gacgaauaac acccuggaau     1500

uucgacgaua aaguggaccg ccaagcauca gcaaaugaau uuauuaaaag aaugacaaca     1560

acagacaccu auuuauuagc cgaggauguc cucccaaaac aaucccuaau cuaucagcga     1620

uuugagguac uuaacgaacu uaacggauua aaaaucgacg accagccuau aacgaccgaa     1680

cucaaacagg cgaucuuuac agaccuguuu augcagaaaa uuucaguaac agugaaaaac     1740

auucaagauu acuuaguaag ugagaaaagg uacgcaucac gaccugcaau cacaggacua     1800

ucagaugaaa acaaauucaa uaguagacua aguaccuacc augauuugaa aaugauaguc     1860

ggugacgcag uagacgaugu cgauaaacaa gcugauuuag aaaaaugcau cgaauggucc     1920

acaauauucg aagaugguaa aauauacagc gcaaaauuaa acgaaauaga cuggcucaca     1980

gaucaacaaa gaguacaacu agcggcaaaa agauacagag gauggggaag auuaagugca     2040

aaacuuuuaa cacaaaucgu uaacgcaaau ggacaaagaa uuauggacuu auuaugggac     2100

accaccgaca auuucaugag gauaguucau ucagaagauu ucgacaaacu aauaacagaa     2160

gccaaucaaa ugaugcuagc agaaaaugac guacaagacg uaauaaacga ucuauauaca     2220

ucaccccaaa auaagaaagc ucuaagacaa auuuuauugg uaguaaauga cauucagaaa     2280

gcaaugaaag gccaagcacc cgaacgaaua uuaauagaau uugccagaga agacgaaguu     2340

aacccccgac uaucaguuca aagaaagcgu caaguggaac aaguauacca aaacauaagu     2400

aaugaacugc uuaauaacac agaaauaaga aaugaacuaa aagauuuauc caauucagca     2460

uuguccaaua cuagauuguu ucucuauuuu augcaaggag gacgugacau guauacagga     2520

gacucacuaa acauugacag acuaucuaca uacgauauag aucacauauu accucaaucc     2580

uuuguaaaag auaacucucu agauaacaga gugcuaguau cccagaaaau gaaucgguca     2640

aaggcagauc aaguaccaac ggauuucacg agcguugaau uaggaaagaa aaugcaacua     2700

caaugggaac aaaugcugcg agcaggauua auaacgaaaa agaaauacga caaccugacg     2760

uuaaaccccg accacaucuc aaaauaugcc augaaaggcu ucaucaaucg ccaacucgua     2820

gagaccagac aaguaauaaa auuagcuaca aaucuccuaa uggaacaaua cggagaagac     2880

aauauagaac uaaucacugu caaauccgga cugacacauc aaaugagaac agaguucgau     2940

uuuccgaaaa acagaaaucu caacaaccau caccacgcau ucgacgcaua cuuaacugca     3000

uuuguaggac uauaccuacu aaaacgguau ccaaaacuaa aacccuauuu uguauacgga     3060

gaauaucaaa aagcauccca acaagauaaa uggagaaacu uuaauuuccu aaauggacug     3120

aagaaagaug aguuaguaga cgaaaacaca gaggcaguaa uuugggacaa agaaucagga     3180

uuagcauacc uaaacaaaau auaucaauuu aagaaaauau uagugacacg agaaguacac     3240

gaaaacagcg gagcccucuu uaaccaaacu uuguaugcag cgaaagacga caaagcauca     3300

ggucaaggug gaaaacaauu aauucccgca aaacaagaua gaccaacugc auuauacggu     3360

ggauauucgg gaaaaacaag cgccuaucua ugccugguca aaaucaaaaa uaaaucagaa     3420

uauauuuauc gcauuuucgg aguaaacaca aguuggucca gugaauuaaa acagauccaa     3480

gacccagccg aaagacuugu acgccuagaa aaauuacuaa cgccacaauu aacaauuaac     3540

aaaaagaaga aaggcgaacc aguacccgua accaaagauu ucaaaaucau acuuccuagg     3600

auauuaauaa aucaaaaauu cuauucucga ggccaagaca uaaguauugc cucugaguuc     3660

uaucuacaca augaacagga gcuaguccug gacaaaagug auaucaaaau ucuaaaugga     3720

gcaauaaaag uaaacaauag caauaaacaa guuauaaacc uuuucaauca uauacuagaa     3780

caaguaaacc aguauuuucc gguuuaugau auuuauaaau ccagggaagu ucuaaacuca     3840

agcaaagaaa uauuugaaaa ucuuaccugg aacgaucaau ggcguaaucg uaaacuaauc     3900

aauguaggac agaaaacuau ccucaaucgc auacuaauag gauuacaugc caacgcagcu     3960

agaauaaaaa uaagugaacu cggauuuaaa aacgacuucg gucucuuaac acaaacaagu     4020

ggaaucaccu ucaccccaga cacucaaauu aucuaccaaa gcccaacggg acuauuugag     4080

cgaagaguag cuuuaagaga ucuauga                                         4107


<210>  5425
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5425
augaaacgau cauauaauau aggauuagau auaggaacaa acucuauagg augggcaaua       60

guugacgacc aaucaaaguu aauaucgguc aaagguaaau auggguacgg aguaagauua      120

uaugacgaag gucaaaccgc cgccgaacgu agaaguuuuc gaaccacaag aaggagacug      180

aaacgacgca aauggcgacu aggccuacua agagaaauau uugaaccaua uauaacucca      240

auagauucga cauucuuuuu acgacaaaag caaucuaacu uaucaccuaa agaucaacgc      300

aagcucuacc cucaaacauc acuuuuuaau gaucgaccag acgcagauuu uuaccaacaa      360

uaccccacaa uauaucaucu ucgcuaucaa cucaugacgg aaaaacgcca auuugacaua      420

cgagaaauau auuuagcaau gcaucacaua guaaaauaca ggggucauuu ccuaaacgag      480

gcaccuguag ccucguuuaa aagcucagaa auuaauuuag cagaacacuu cgaccaacug      540

aauaccauau ucgccgaucu auuuagagaa uccggauuuc aacucgagac agacgaauua      600

guugccguaa aagcacucuu gauggauaac caacaaagug cgucaaaccg acaacgaaaa      660

gcauugccaa aacuauacac gccaucagcg gacaaaagua uagaaaaaca aagaaaggca      720

auagccacag aaauacuaaa ggcaauucua ggauuaaaag caaaauucaa cguugugacu      780

ggaauugaag ccgaagacgu gaaagccugg gcccucaccu ucaaugcaga gaauuuugau      840

gaagaacuug caaaacuaga gaguucauua gacgacaacg gccacgaggu aauaggaauc      900

uuacaagaau uauacuccgg aauauuacua gccgguauag ucccagaaaa ucaauccuua      960

ucccaagcua ugauuacuaa auaugaagaa caccaagcac acuuaaaaau gcuaaaagcc     1020

guaagagaug aucuagcgac ggccgaccaa caaaaguuaa agaaagcaua cgaucaauau     1080

aucgacggcc aagaaaauac uaaagcauac ucaaaagagg cuuucuacga cgaugucaca     1140

aaagcacuaa aagacuacag cgaacaaccc uuaguaagug aaauucaaaa auuaauugaa     1200

uuagaccaau ucaugccuaa acaaagaacu aaagauaacg gcgcuauacc ucaccaacuu     1260

caucaacaag aauuagaccg aauaauagaa aaucaacagc aauauuaucc uuggcuugcu     1320

gaauuaaacc cgaauccaaa aagacaaaca guagcuaagu auaaauuaga ugaauuagua     1380

gccuuucgag ucccauacua ugugggacca uuaauaaccg cggaacaaca gcaacaaagc     1440

ucaaaugcca aauucgccug gaugauuaga aagaccgaag gccgcauuac accauggaau     1500

uucgaugaua aagucgaucg acaagccagu gcaaacgaau uuauaaaaag aaugaccaca     1560

acagacacau aucuuuuggc ggaagacgua cuaccaaaac agucacuaau cuaucaacgu     1620

uucgaaguac uaaaugaacu uaauggccua aagauagaug accaaccaau aacaaacgaa     1680

uuaaaacaag cuauuuuuaa cgauuuauuu augcagaaaa cauccguauc ggucaaaaac     1740

auucaagauu acuuagugag ugaaaaacgu uacgcaucgc guccagcgau uacaggccuc     1800

ucagaugaaa agaaauucaa caguagauua uccacauauc augaccuuaa agucauugua     1860

ggagaugcug uagacgacgu agacaagcaa gcugaccuag aaaaguguau cgaauggagc     1920

acgaucuucg aagauggaaa gauauauagc gcaaaacuaa augaaauuac augguugacc     1980

gaacaacaaa gaggacaacu cgcaacgaaa agauaucgcg gauggggcag acuuuccgca     2040

aaauuauuaa cgcaaaucgu gaaugcaaac ggacaacgaa uuauggaucu ccugugggac     2100

acaacugaca acuucaugcg cauagugcac uuugaagacu ucgacaaauu aauaaccgaa     2160

gcgaaucaaa ugguuuuggc agaaaacgac guacaagacg uaauaaacga ucuauauaca     2220

aguccccaaa auaagaaagc ccugagacaa auucuauuag ucguuaacga cauacaaaaa     2280

gccaugaaag gucaagcucc cgaacguauc cuaaucgagu ucgcccgcgg ggcucaaaaa     2340

gacaaaucac uaacggugag acgaaaacgg aaacuagaug aaauguauuc ccaaauauca     2400

gacgcuguuc uagccgauga auccguaaga caagaacuaa aaucaagcaa ccaagaugac     2460

cuaguaaaag aaaaaguuua uuuguauuuu augcaaggag gaagggaucu auauaccgga     2520

aaugcucuaa auauugaucg auuaucagau uaucacguag aucacauaau accacaagca     2580

uacaucaaag augacagccu agauaauaaa gcucuaguua gcucaagaau gaaugaaauc     2640

aaagcagaca gggucccccu acaagucgua caaaaacaau uugguaaaaa cauggcaagu     2700

gaauggggau acaugcaccg agcaggacuc auaucuagaa agaaauauga uaaucuaaug     2760

uucaacccag aaaaucuaaa uaaauaucaa auuaaucgcu ucauaucucg acaauuagua     2820

gaaacucaac agguaauaaa auuaguugca aacuuauuau caaaucagua cgaaggagac     2880

aaugucaaaa uuauaaccgu aaaaucaaaa cuuacacaaa caauaagguc agaauuugac     2940

uuucccaaaa auagggaggu aaauaacuac caccaugcau uugacgcaua uuuaacagca     3000

uucguuggaa uguaccuucu aaaaagauac ccaaaauuau caucauauuu cacauacgga     3060

gaauaucuca aaggaaacca acaagauaaa uggagcucau ucaauuuccu aaacgaucuc     3120

aaaagagcca aacucgaaga uaaaacuaac aaagaaaucu uuuggcacaa ggagucaggu     3180

cuagcauacc uuaacaaaau uuaccaguuu aagaaaauuc uaguaacacg agaaguucau     3240

gaaaacucag gagcauuauu uaaccaaaca cuauaugccg caaaagauga caaagccucc     3300

ggacaaggag gaaaacaacu gauaccagcu aaacaagcca gaccaaccgc cuuauacggc     3360

ggauauagcg gcaaaacugu ggcuuacaug ugcauuguca gaauaaagaa caagaaagga     3420

gaucucuaua aagucugcgg aguagaaaca agcugguuag cucagcuuaa acaguugaaa     3480

gacgaagauu cacaaaaagc guucuuaaaa cagaaaauaa gcccacaauu caccaaagca     3540

aagaaacaaa aaggaacaau aguaaaaguc gucgaggacu ucgaaguaau agcgccacau     3600

auacuaauaa accaacgacu auuugacaac ggacaagaac ucacauuagg uagcgcaacg     3660

uacaaacaua acgaacaaga acuuauacua gauaaaacug cgguaaaacu guugaacgga     3720

gagcuccccc ucgcacaaag cgaagaacua gccgaacugg uauacgauga aauacuagau     3780

caaguaaugc acuacuuccc auuauaugac acgaaucaau ucagagcaaa auuacucgcu     3840

ggcaaaacag ccuuuaugca guuaccuugg aaaagcaauc cagacgauaa caaaacagug     3900

caaauuacuc aacccauagu gcucaagaaa aucuuacaag gauuacaugc agaugcaggc     3960

agagucucac ucaaagaacu ugggaugaca accgauuucg ggaugcuaca aaaagcaacu     4020

gggauaacau uuacaccuga cacccaaauc auauaccaaa guccuacagg auuauucgag     4080

agaagaguag cccuaaggga ucuauga                                         4107


<210>  5426
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5426
auggauaaaa aauauaguau aggucuagac auuggaacua acucuguugg cugggcagua       60

auaaccgaug acuauaaggu cccaucaaaa aaauucaaag uucuuggaaa cacagauagg      120

cacaguauaa aaaaaaaucu cauaggagca uuacuauuug acucaggaga gacggccgaa      180

gcuacuagau uaaaaagaac agcaaggaga agauauacca gaagaaagaa uagaauaugc      240

uaccuacagg agauuuucuc caaugaaaug gcaaaaguag acgauaguuu cuuucacaga      300

uuggaagaau cauuccuugu cgaagaagac aagaaacacg aacggcaucc aaucuucggc      360

aacaucguug acgaaguggc auaccaugaa aaauauccaa caauauacca ccuaagaaaa      420

aaauuaguag acucaacuga caaggccgac cuccgacuaa uauaucucgc ucucgcucau      480

augauaaaau uucgaggcca cuuccuuaua gaaggcgacc uaaacccaga uaauucugau      540

guagacaaac ucuucauaca acucguacaa acuuauaauc aauuguuuga agagaaccca      600

aucaaugcca gugguguaga ugcaaaggcu auccuaucag caagauuguc aaaaucccgg      660

agacuagaaa accuaauagc ucagcucccc ggcgaaaaaa aaaacggacu auuugguaac      720

cuuauagccc uaucauuagg ucuaacacca aacuuuaaau cgaauuuuga ccuagcugaa      780

gaugcaaaac uacaauuauc aaaagacacc uaugacgacg aucuagauaa cuuacucgcu      840

caaauaggcg accaguaugc ggauuuauuu cucgcagcua aaaaucuuag ugaugccauc      900

cuguuaucag auauacucag ggucaauaca gaaauaacaa aagcaccccu aagcgcaagc      960

augauuaaga gauacgacga acaucaucaa gacuuaacau uacugaaagc ccuaguuaga     1020

caacaauuac cggagaaaua caaggagaua uucuuugauc agucuaaaaa cgguuaugca     1080

ggcuacauag acggaggcgc uucccaagaa gaauuuuaua aauucauuaa accaauccuu     1140

gaaaaaaugg acggaacaga agaauuacua guaaaacuga acagggagga ccuuuuaaga     1200

aaacaaagga cauuugacaa cggaagcauc ccccaccaaa uacaucuugg agaacuacac     1260

gcaauacuac gaagacagga agauuuuuac ccauuuuuga aagacaauag agaaaaaauu     1320

gaaaaaauuc uaacauuuag aaucccauac uacgucggac cccuagcaag gggcaacucu     1380

agguuugcgu ggaugacaag aaaaucggaa gaaaccauua caccauggaa cuucgaggag     1440

gugguagaua aaggagcaag ugcacaaucc uucauugaaa gaaugaccaa uuuugauaaa     1500

aaucuaccaa acgagaaagu ccuaccgaaa cacucauuac uuuacgagua uuucacuguu     1560

uauaaugaac ucacaaaagu uaaauacgua acggaaggca ugagaaaacc agcauucuua     1620

ucaggugagc aaaagaaggc aauaguagac uuauuauuua aaacaaaccg aaaaguaacc     1680

guaaaacaau ugaaagaaga cuacuucaaa aaaauugaau gcuuugauuc agucgaaaua     1740

ucaggaguag aggacagguu caacgccuca cuagggacau aucacgaucu ucuaaaaaua     1800

aucaaagaca aagacuuccu cgacaaugag gaaaaugaag acauauuaga agacauagua     1860

uuaacguuaa cacucuuuga agauagagag augauagaag aaagacuaaa aaccuaugcg     1920

cauuuauucg acgauaaagu uaugaaacag cugaaaagga gaagauauac agguugggga     1980

agacuaucua gaaaauuaau aaacggaauu cgcgacaaac agucaggcaa gacaauccuu     2040

gauuuucuca aaucugaugg uuucgcaaau cgcaauuuua ugcaauuaau ucacgaugac     2100

agucucacuu ucaaagaaga uauccaaaag gcacaaguca gcggacaagg cgauagcuug     2160

cacgaacaua ucgccaaucu agcaggaagc ccagcaauaa agaaaggcau ucuacaaacg     2220

guuaaaguag ucgaugaacu aguaaaaguu augggacgac acaaaccaga aaacauaguc     2280

auagaaaugg cucgggaaaa ucaaacaaca caaaaaggac agaaaaacuc acgcgaaaga     2340

augaaacgua uagaagaagg aauuaaagaa cugggauccc aaauucucaa agaacaccca     2400

guagaaaaca cucaacuuca aaacgagaaa uuauaucuau acuauuuaca aaacggcaga     2460

gauauguacg uugaccaaga auuagacauc aacaggcucu ccgacuacga cguagaccau     2520

auaguaccac aaagcuuucu uaaagaugac ucaaucgaua acaaaguacu cacccguucu     2580

gacaaaaaca gaggcaaaag cgacaacgua ccgucggaag agguaguuaa aaaaaugaag     2640

aacuauugga gacaacuacu uaacgcaaaa cuaauaacac aacgaaaauu ugauaacuua     2700

accaaagcag aaagaggcgg acuaucagaa uuagacaaag ccggcuuuau aaaaagacaa     2760

uugguagaaa ccagacaaau uacaaaacac guagcacaaa uacucgacuc acguaugaac     2820

acaaaauaug acgaaaauga uaaacuaauc agggaaguca aaguaauaac auuaaaaucc     2880

aagcuaguau cagauuuuag aaaagauuuu caauucuaua aagucaggga aauuaacaac     2940

uaccaucaug cacacgaugc cuauuuaaac gccgucguug ggacagccuu aauaaaaaaa     3000

uaccccaaau uagaaagcga auucguuuac ggugauuaua aagucuacga cguacgaaaa     3060

augauagcca agucagaaca agaaauagga aaagcaacag cuaaauacuu uuuuuauucc     3120

aacauaauga auuuuuucaa aacagaaauu acccuagcca acggugaaau aagaaaaaga     3180

ccauugauag aaacaaacgg agaaacugga gaaauuguau gggacaaagg aagggacuuu     3240

gcaacgguuc gcaaaguucu aaguaugcca caagucaaca uaguaaaaaa aaccgaagua     3300

caaacaggag gcuuuucaaa ggagucaaua cuacccaaaa gaaacucaga caaauuaauc     3360

gcucgaaaaa aagauuggga uccaaaaaaa uacggcggau ucgacucccc uaccguugca     3420

uauucagucu uagucguugc gaaaguagaa aaagguaaau caaaaaaauu aaaaagcgua     3480

aaagaguuac uaggaauaac caucauggaa cgaagcaguu ucgagaaaaa cccuauagac     3540

uucuuagaag cgaaaggaua caaagaggua aaaaaggacu ugauaaucaa acuuccaaaa     3600

uacucucucu uugaacuuga aaauggcagg aaaagaaugu uagcaucagc aggugagcuu     3660

caaaaaggca acgaauuagc acucccuucg aaauauguga acuuccucua ccuagcuucc     3720

cauuaugaaa aauuaaaagg aaguccagaa gacaacgaac aaaaacaacu auuuguggaa     3780

caacacaaac auuaccuaga cgaaauuaua gaacagauau cagaauuuuc aaaaagagua     3840

auacuugcug acgccaaucu agauaagguu uugagcgcau auaauaaaca cagagacaag     3900

ccuauaagag aacaagcgga aaauauaaua caucuauuca cacuaacgaa ucucggugca     3960

ccugcagccu ucaaauacuu cgacacaaca auagaccgaa aacgauacac aucaacgaaa     4020

gagguuuuag augcuacacu uauucaccaa ucuauaacag gacuauauga aacucguaua     4080

gacuuaucgc aacucggcgg cgacuga                                         4107


<210>  5427
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5427
auggacaaga aauauaguau aggucuugac auaggaacaa acucaguagg gugggcagua       60

auuacggaug acuauaaagu uccauccaag aaacuaaaag gccugggaaa uaccgauagg      120

caugguauaa agaaaaacuu gauaggagcu cuacucuucg auuccggaga aacagcugag      180

gcgacccguc uaaaaagaac agcaaggaga agauauacac gaaggaaaaa cagaauuugc      240

uaucuacaag aaauauucuc caaugaaaug gcaaaagucg acgauucauu cuuucacaga      300

cuagaagaga gcuuucuugu ugaagaagau aagaaacaug aacggcaucc aauauucgga      360

aauauuguag augaaguugc uuaucaugaa aaauacccca caauuuauca ucugcguaag      420

aaacuagcag acaguaccga uaaaguagau cuccgccuua uauaucucgc acuagcucac      480

augauaaaau ucagagguca cuuccuuauu gaaggagauc uaaauccuga uaacucagau      540

gucgauaaac uauucauaca gcuaguacaa acuuacaauc aacucuuuga agaaaacccc      600

auuaacgcau cucgaguaga cgcaaaggcu auucuuucag cgagauuauc uaaaucaaga      660

cgguuagaaa accuuauagc gcaacuuccu ggugaaaaga aaaacggccu auucgguaau      720

cuaauagcuc uaucacuggg acuaacgcca aauuuuaaau ccaacuuuga ucuagccgaa      780

gaugccaaac uccaacuauc aaaagauaca uacgaugacg accuagacaa ucuacucgca      840

caaauaggag accaauacgc agauuuauuc cuagcagcga aaaacuuauc cgacgcaaca      900

cuacuaucug acauuuuaag aguaaacucu gaaauaacaa aagcaccucu cucagcaucc      960

augauuaaaa gauacgauga acaccaucaa gauuuaacac uccuaaaggc ucuaguaaga     1020

caacaauuac ccgaaaagua caaggaaaua uucuuugacc aaucaaagaa cggauaugcg     1080

ggguacauag acgguggcgc cucacaagaa gaauuuuaua aauuuauaaa acccaucuua     1140

gagaaaaugg acgguacaga agaauuauua gcuaaauuaa accgagaaga ucuuuuaagg     1200

aaacaaagaa cuuuugacaa cgggucaaua cccuaucaaa uccacuuagg agaacuacac     1260

gcaaucuuaa gaagacaaga agacuuuuac cccuuuuuaa aagacaauag agagaagauc     1320

gaaaagauau uaaccuuuag aauaccuuau uaugucggac cacucgcaag aggaaacuca     1380

agauucgcau ggaugacccg aaaauccgaa gaaacaauaa caccuuggaa uuuugaagaa     1440

guaguagaca aaggagcauc cgcacaauca uuuaucgaac gaaugacuaa uuuugacaaa     1500

aauuugccca augagaaagu cuuacccaaa cauucauugc uauaugaaua uuuuacggua     1560

uacaaugaac uuacgaaagu uaaauacgua accgaaggaa ugagaaaacc agcauuucua     1620

uccggggaac aaaagaaagc aauaguagau cuacuauuua aaacuaauag aaaaguuacc     1680

gugaaacaau uaaaagaaga uuauuuuaag aaaaucgaau gcuuugacuc gguagaaaua     1740

ucuggcguag aggaccgcuu caacgccuca uuaggcacau accacgaccu acuuaaaaua     1800

auaaaagaua aagacuuucu ggacaaugag gaaaaugaag auauacuaga agauauuguc     1860

cuuacccuua cacucuucga agacagagaa augauagaag aaagacugaa aacuuacgcc     1920

caucucuucg acgauaaagu aaugaaacaa uuaaaaagga gaagauacac aggaugggga     1980

cguuuaagua gaaaacuuau uaacggaauc agggauaaac aaagcgguaa aacaauacua     2040

gauuuccuaa aaagcgaugg guucgcuaau agaaauuuca ugcaacugau acaugacgac     2100

ucauuaacgu uuaaagaaga uauacaaaaa gcacaaguau caggucaagg agacucguua     2160

caugaacaca uagcaaacuu agcaggaucu ccugcgauaa agaaaggcau acuacaaaca     2220

guaaaagugg uagacgaauu agucaaaguc augggccguc acaaaccaga gaacauaguu     2280

auagaaaugg cuagagaaaa ucaaacgaca caaaaaggac agaaaaauuc acgcgaacga     2340

augaaacgua ucgaagaagg gauaaaagag uuaggcucag acauacuaaa agaauaucca     2400

guagaaaaca cacaauuaca aaaugagaaa cuauaccuau auuauuuaca aaauggaaga     2460

gacauguaug ucgaucaaga acuugauaua aaucgacuau cagauuacga uguagaccac     2520

auaguaccac agagcuucuu aaaagaugac uccauugaua auaaagucuu gacucgauca     2580

gauaaaaaca gaggaaaaag cgauaauguc ccaagugagg aaguaguaaa gaaaaugaag     2640

aacuauuggc gacaauuacu uaacgcgaaa cuuaucaccc aaagaaaauu cgacaaucuu     2700

acuaaagcag aaagaggagg ccuauccgag uuagauaagg uuggauucau aaaaagacaa     2760

cugguagaaa caagacaaau aacuaaacac gucgcccaaa uacuagacuc acgaaugaac     2820

acuaaauaug augaaaacga uaaauugaua cgcgaaguca gaguaauaac ccuaaaaucg     2880

aaacuuguau cagacuuuag aaaagauuuc caauucuaua aaguacgaga aauaaacaac     2940

uaucaccacg cucaugaugc auaccuaaac gcaguuguag gaacggcauu aauaaagaaa     3000

uauccaaaac uagaaucuga auuuguauau ggugauuaua aaguauauga cguacggaaa     3060

augaucgcua aaucagaaca agaaauaggu aaagcuacag caaaauacuu cuuuuauagu     3120

aauauaauga acuuuuucaa aacagaaauu acauuagcga acggggaaau aagaaaaaga     3180

ccguuaauag aaacaaacgg ugagacuggc gaaauaguau gggauaaagg acgggauuuu     3240

gcaacaguga gaaaaguccu aucaaugcca caagucaaua uugugaagaa aaccgaggua     3300

caaacaggag gauucucuaa agaaaguauu uuaccuaaaa gaaauaguga caaacuuaua     3360

gcuagaaaga aagacuggga uccaaagaaa uacggagguu uugauagccc uacaguagcu     3420

uacagugucu uaguagucgc aaaaguagaa aaaggaaaau ccaagaaauu aaaaucagua     3480

aaagaacucu uaggaauuac aauaauggaa cguaguucau uugagaaaga uccaauagac     3540

uuccuggaag ccaaaggaua caaagaaguu agaaaagauc uaauaaucaa auuaccuaaa     3600

uacagucucu uugaacucga aaauggcagg aaaagaaugc uagcaagugc cggggaacua     3660

caaaaaggaa augaacuagc cuuacccuca aaauauguaa acuuucuuua ucuggcaagc     3720

cacuaugaga aacuuaaagg aucaccagaa gauaaugaac aaaaacaauu auucguagaa     3780

caacacaaac acuaucucga ugaaauaauu gaacaaauaa gugaguuuag uaaacgagua     3840

auauuagcgg augcaaaucu agauaaaguc cuaucagcgu auaauaaaca ccgagauaaa     3900

ccaauaaggg aacaagccga aaacauaauu caccucuuca cacuaaccaa ucucggggcg     3960

ccagcggccu uuaaauauuu cgacacuaca auagaucgaa agagguauac aagcacaaaa     4020

gaaguacucg acgccacucu cauacaucaa ucaaucaccg gauuauauga aaccagaaua     4080

gacuuaucgc aacuaggugg cgauuga                                         4107


<210>  5428
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5428
auggacaaga aauauucaau ugguuuagac auugguacaa auagcguagg augggcaguu       60

auaacggacg acuauaaagu cccuucaaag aaauucaaag uccuaggaaa cacagacaga      120

cauaguauua agaaaaaucu caucggugca uuacuauuug auaguggcga aacugcggaa      180

gcuacgcgac ucaaaagaac agcgcgacga agauacacua gaaggaaaaa cagaauuugc      240

uaccuacaag aaauauuuag caacgaaaug gcaaaaguug acgauagcuu uuuccacaga      300

cuagaagaaa guuuucuugu agaagaagac aagaaacaug aacgacaucc aauauucggc      360

aacauaguag acgaaguggc auaucaugaa aaauacccca cuaucuauca uuuaagaaag      420

aaacuggcag acucaacgga uaaagcagac uuaagacuca uauauuuggc guuggcacau      480

augauaaaau ucagaggaca cuuuuuaaua gagggcgacu uaaacccaga caacagugac      540

guugauaaac uauucauaca auuaguccaa acauauaauc aauuauucga agaaaacccc      600

aucaaugcau caagaguaga ugccaaagcu auacucucag cuagacuauc uaaaaguaga      660

agacuagaga accuaaucgc ccaauuacca ggagagaaac gaaacggauu auuuggaaau      720

cucauagccu uaucauuagg acuaacgcca aauuucaaaa guaacuucga cuuagcagaa      780

gaugcaaaac uacaauuguc caaagacaca uacgacgaug auuuagauaa ccugcuugca      840

caaauaggag aucaguacgc cgacuuauuu cuugcagcga aaaauuuaag cgaugcaaua      900

uuacuuucag auauacuacg gguaaacucc gaaauuacua aagccccucu uagcgcgagu      960

augaucaaaa gauaugauga acaucaucaa gaccuaacac uauuaaaagc ccuuguacga     1020

caacaguuac cugaaaagua caaagagauc uucuuugacc aaucgaaaaa uggauacgca     1080

ggauacaucg augggggagc aucacaagaa gaguuuuaua aguuuauaaa gccaauucua     1140

gagaaaaugg acgguacaga agaauuacua gccaaauuaa acagagaaga ucuacuaaga     1200

aaacaacgaa cauuugauaa ugguucaaua ccacaucaaa uacauuuagg agaauuacac     1260

gcaauacuuc gccgacaaga agacuuuuac ccauuucuua aggauaaucg agagaaaaua     1320

gaaaagaucu uaacauuccg aauaccauac uacguugguc ccuuagccag aggaaauuca     1380

agauucgcau ggaugacaag aaaauccgaa gaaacgauua caccguggaa uuucgaagaa     1440

guagucgaca aaggagccuc cgcccaaagu uuuauagaaa gaaugacaaa uuucgauaaa     1500

aauuuaccca acgaaaaggu ccuaccaaaa cacagucuac uauaugaaua uuucacagua     1560

uauaaugaac uaacaaaagu gaaguaugua acagaaggaa ugaggaaacc ugaguuccua     1620

ucaggagaac agaagaaagc cauaguagau cuauuauuca aaacgaauag aaaaguuacc     1680

guuaaacaac uaaaagaaga cuauuucaag aaaauagaau guuucgauuc aguagaaaua     1740

aguggaguug aagauagguu caacgcuucu cuaggaacuu accacgaccu ucucaaaaua     1800

auaaaagaca aagacuucuu ggacaaugaa gaaaacgaag auauacuaga agacauaguu     1860

cuaacucuca cacuauuuga agaccgggaa augauagaag agaggcucaa aacauaugca     1920

cacuuauucg acgacaaagu aaugaaacaa uuaaaaagac guagauacac aggcuggggu     1980

aggcuuucaa gaaaacuaau uaacgguaua agagauaaac agucaggcaa aacgauucua     2040

gauuucuuaa aaagugaugg uuucgcgaau agaaauuuua ugcaauugau acaugacgau     2100

agccuaacuu uuaaggaaga uauacaaaag gcccaaguua guggacaagg agacucacua     2160

cacgaacaca uagcaaauuu agcagguuca ccggcgauca agaaaggaau cuuacaaaca     2220

gugaaaguug ucgaugaauu aguuaaagua auggguagac acaaaccaga aaacauaguc     2280

auugaaaugg caagagaaaa ucaaacgaca caaaaaggac agaaaaacuc acgggaaaga     2340

augaaacgga uagaagaagg gauaaaagaa cuuggaucag acauucucaa agaauacccc     2400

guugaaaaua cacaacucca aaacgaaaaa cuauacuuau auuaucuaca aaacgggcgu     2460

gauauguaug uagaccaaga auuagacaua aacagguuaa gcgacuacga ugucgaccau     2520

auaguaccac agagcuuuau aaaagacgac ucaauagaca acaaaguucu aacucgaagu     2580

gacaaaaaua gaggaaaaag ugacaacgua ccaucagaag aaguaguuaa gaaaaugaaa     2640

aauuacugga aacaacuuuu aaaugcaaaa cuaaucacac aaagaaaauu ugacaauuua     2700

acaaaagcag aaagaggagg ccuaucagag cuggacaaag caggcuuuau aaaaaggcag     2760

cuaguugaaa cgagacaaau uacaaaacau guugcgcaaa uccuagauag ccguaugaac     2820

acaaaauaug augaaaauga caaguuaaua agagaaguua aagugauaac ucucaaaucc     2880

aaacucguau cagauuuccg aaaagacuuu caauuuuaca aagucagaga aaucaauaau     2940

uaucaucaug cacacgaugc auauuuaaau gccguaguug ggacagcguu aauuaagaaa     3000

uauccaaaau uagaaaguga guuuguuuac ggcgacuaua aaguguacga ugugcgaaaa     3060

augauugcca aaucagaaca agaaauagga aaagccacag cuaaauacuu cuucuacuca     3120

aacaucauga auuucuucaa aacugaaauu acgcuugcaa acggagaaau aagaaaacga     3180

cccuuaauag agacuaacgg agaaacaggu gaaauuguuu gggacaaggg cagagauuuu     3240

gcuacuguaa gaaaaguacu aagcaugccc caaguuaaua uugucaagaa aaccgaagua     3300

caaacaggag gguuuuccaa agagaguaua cuuccaaaaa gaaauucaga caaauuaaua     3360

gcgagaaaga aagauuggga cccgaagaaa uacgguggau ucgacagccc aacaguagca     3420

uauucaguuc uaguuguagc aaaaguagaa aaaggaaaaa guaagaaacu caaaucagua     3480

aaagaauuac uagguauuac aauaauggaa agaucaucau uugagaaaaa cccuauagau     3540

uucuuggaag cuaaagguua uaaagaagua agaaaagacu uaaucauaaa acuuccaaaa     3600

uauucacucu ucgaauugga aaacggcaga aaaagaaugu uagcaucagc aggagaauug     3660

caaaaaggaa acgaauuggc acugccaagu aaauauguua auuuucuaua ccuagcaagu     3720

cacuacgaaa aacuaaaagg aagcccugaa gauaaugaac aaaaacaacu uuuuguagaa     3780

cagcauaaac acuaccuaga cgaaauaauu gaacaaauaa gugaguucuc aaaaagagua     3840

auucuagcgg augcaaacuu ggauaaagua cuaucugcuu auaacaaaca cagagacaaa     3900

ccaauaagag aacaggccga aaacauaauc caucucuuua cucuaacuaa cuucggagca     3960

ccagcagcau uuauauauuu cgauacaaca auaggaagaa auagauauaa aaguauaaaa     4020

gaaguucuug augcuacuuu aauccaucaa aguaucaccg gacuguacga aacaagaaua     4080

gaucuaagcc aacuaggagg agauuga                                         4107


<210>  5429
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5429
augaaguaug aaucugaaga auauuacauu ggacuugaca ugggaacauc aucagucgga       60

ugggcaguaa ccgauccgca uuaccaucua auccgaaaaa aaggaaaaga ucucugggga      120

gcacgccucu uugaaaaagc aaacacugcu caagagaggc ggaccaaccg aaucaacaga      180

cgucgaaauc aacgcaggaa acaacgcauc gcauucauac gagaagcuuu uagaaaagaa      240

guagaaaaag uagauguaaa uuuuuuucga agacuugacg aaucucaauu auauuuagaa      300

gauaaaucaa uaaaggaacc auaucuacuu uuuaacgacu uaaauuauac agacaaggac      360

uuuuaccauc aauacccaac gguguuucac cuaauaacag accuauuaac aucagaccgc      420

aaauucgaua uucgagaaau cuacauagca guuucaaguc uauacaacca cagaggcaac      480

uuccuaaaca aaaguuuagg aaaugaaacu caggacgaag accuuguauu caaaaauuua      540

gaugaagcuu ucuccgccug cgguaugaac uggaacacca aagcagaaaa uaucgaagua      600

guaaaacaaa uucuuuguga cagaagccua aauaaaaagg aaaaacaaga ccaacuagca      660

cacugccuug agauuaacaa aaaaaccgaa aaagaaaaaa ucgaauuucu caaacuacua      720

acaggaggaa aagcagauuc caaaaaaaug uucccacagu accacuauga acgagacgaa      780

aaauuauccu ucgcguuugc agaugaaaac ggcgacgaaa auugggaaga aacauuaaac      840

gcaauugaag aaagacuaac uucagaugaa uacgggcuaa ucuuagcacu caagagcguu      900

uaugauuggu ccauauuagc cgaccuaaug aaaaaugaaa aaacgggaga aacauaccca      960

uaccuaucaa uggcaagaaa agacuccuau gaaaaacaca aagcugaucu aagguugcua     1020

aaaaguguaa uaaagagacu cauaccuaac gaauacaacc acuucuuucg gcaauaugac     1080

gguaaaggca aaacguacac aaacuauaua ggcucaguuc aguaccauga aaaaagacau     1140

agauucaagc auauaacaac aaaagaauuu uaugauggau uaaagaaacu acuuaaaccu     1200

uauucagaag augaagaaau uaaacaaauc cuacaagaua uagagucaga aacuuucuug     1260

ccgaagcaaa uaacuaauau gaacggagua aucccaaauc aaguccaugc caaagaacuu     1320

aaaguaauau uagagaaagc cucucaacau uaugauuucc uuaaugaaac aaacgaaucu     1380

ggauugacag uaaaagaaca aauaaugcaa auguuuacau uucagauacc uuacuaugua     1440

gggccucuug caaaucucaa uggcaaaggc aauggauggg cuguaagaua cguagggaaa     1500

gaaagaacaa aagucuaucc auggaauaug gacgaaguca uagacgaaaa agaaacagca     1560

gaucaauuua uaaaaaggau gauaucaacc uguacuuauc uaggagacga auacguacua     1620

ccaaaaucau ccauucuaua ugaaaaguuc gcagucuuaa acgaguuaaa cucacuuaaa     1680

aucaauggac aaccagucuc gguugacuua aaacaaaaaa uauacagaga uuuauucaca     1740

aaaggcaaac gcguaacaca aaagggacuu uuacuuuacc uaaaaaggga aggagucaua     1800

ucaaaaagug aagagacaau auugaguggg uuugauauug acuucaaaaa uacucuauca     1860

ucuuaucgca aauucuguga agucuuugca acaaacgaac uuaacgacga acaaauccgc     1920

auggcagaag acauaauaga auggcuaacc gucuucggag auucuaagaa auucguaagg     1980

gaaaaaauac auggaaauua cccacaaaua acagcaaauc aacuaaaaag aauacuauca     2040

uuuaauuuca aagacugggg aaaacuauca aaacaauuuc uggaauuaga aggagaagau     2100

agagaaacgg guaaaagcug uucucucaua caagcgcucu gggacacaaa uaacaacuua     2160

aaccaacuau uaucauccag auuuacauau aaagaaaauc uagaaaagaa acguaaaaaa     2220

auagagaugg aucuauuuga auucacucau gaggauuuag acgaccuuua uauuucagca     2280

ccaguaaaac gaaugauaug gcaagcauua aaaauaauga aggacauagu aauaguaaug     2340

aaagaauccc caacuaaacu cuucauugaa augacaagaa acaacgaaaa ugaaaaaacc     2400

cgaacaguau caagaaaaaa caaacuuguc gacuuauaua agaaaauaaa gggagaauca     2460

cgagauugga uaaaagaacu agaacguuau gaagaaaaag auuuucgaag uaaaaaauua     2520

uacuuauacu auacacaaau ggggaaaagu gcuuauucag gagaaccuau ugacauggau     2580

aaacucuucg auaaaaacgu cuacgacaua gaucacauau acccaaaauc aaaaguaaag     2640

gaugauaguc uauugaacaa uugcguucua guaaagagaa aagaaaacau ggauaaagga     2700

aacaauuuuc cuauugaccc agacauacag acaaaacaag aacaucuuug gaaauucugg     2760

cuagaaaagg gacucauaac ccaagaaaaa uucaacagac uaaccagaaa gaaacccuuu     2820

acuaaugacg aacuugcuga cuuugucaac cgacaacuag uagagacagg acaagccacc     2880

aaaaccauug cggaucuguu caaaaugaca uuuccaaaca gcgacaucgu auauguaaaa     2940

gcaggaaacg uaacugauuu cagacaauac auagaagucu acaaaugccg agaaguaaau     3000

aaccugcacc acgcauauga ugcuuacuua aauguaguag ucggcaacgu uuacgauacc     3060

aaguucacaa aaaacccuau gaacuacauc aaagaacaaa ggaaagaaaa cucaaaugaa     3120

cgguaucaua uggacaaaau auucgaauuu cccgugauac gcaacggcaa agaagcaugg     3180

auaacaaaaa aagaugaaag uauaaacauu guaagaaaaa cacuagcaag aaaaacgccu     3240

uuaguaacaa aacgaaauuu cgaagcaaau gggacaauag ccaaagcaac uaucuaccca     3300

aaaggcaacg ucaaagacca gggauaccua ccaagaaaaa ccuccauucc agcacuaaga     3360

gacaugaaaa aauacggugg uguaaccagc auacaaauag cauacuuuuu ucuuguugaa     3420

cacgaaguua agaaaaaacg cauccggaca auugaaugug uaccgaucua ucgaaaagaa     3480

gaacugaaga caaaagaaca acuagaacga uauuguagag aggaacuaaa acuuauaaac     3540

ccaucgauua ggcuugaaaa gauaaaacca uacucacuac uuaaaaugaa cggaaacuuu     3600

uucuacauaa caggaaaaac caacgauaga uucagucuug ccaaugcaau ggaguuauau     3660

guggacaaag uuucugaaaa ccacauaaga gaccuaguaa aauauaaaaa uugcggauac     3720

uuuaacgucg acgauaaaga agccagaaaa cgaaaugaac gucuauacac aauacuuaca     3780

aaaaaacauu uaacugucuu caaaggcagc cccaaaccaa ugggagaaaa acuucaacaa     3840

uaucaacaac ucuuucuaga acucgagguu agcaaacaag uauacguccu ucuacaaaua     3900

uuacaacuua ccgcuacuaa cgugaaaacc aaagcugacc ucaccgcgau cggaggaaaa     3960

gcgaacgaag gcacaaugga uauaagcaaa aaucuagaaa acgucauaaa aaaucaaauu     4020

aaaaaaggac ucguaccaga gcuaaugcug aucaaucaau caccaacagg acucuuugaa     4080

uccgaaaucg accuauuaac gaucuga                                         4107


<210>  5430
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5430
augagcaggc cauacaacau aggucuggac aucggaacaa gcagcauagg auggucagua       60

guggaugauc aauccaaauu agucucagua cguggaaaau acggcuacgg aguucgccua      120

uacgaugaag gccaaacagc agcggaaaga cgcuccuuca gaacaacuag aagaagacua      180

aaaagacgaa aauggcgacu cggauuacua agagaaauau uugaaccuua cauaacacca      240

guagaugaua cuuucuuuuu acgaaagaag caaucaaacu uaucaccuaa agaccaaaga      300

aaauuauacc cacagacguc ccucuucaau gacagaaccg aucgagccuu cuacgaugac      360

uacccgacga ucuaccaccu acgcuauaaa cuaaugacug agaaaagaca auuugacaua      420

cgagaaauau aucuagcaau gcaucacaua gucaaauauc gcggacacuu ccuaaaugaa      480

gcaccuguau cgucauuuaa aagcucagaa auaaaucucg uagcccacuu cgacagauua      540

aauacuauau ucgcagaccu auuuagcgaa ucaggguucc aacucgaaac ugauaaacua      600

gcagagguaa aagcauuacu acucgacaac caccagucug caaguaaucg ccaaagacaa      660

gcccuauuac uaauauauac uccuucaacc aauaaagcug uagagaaaca gaauaaagca      720

auagccacag aacuacuuaa agcaauacua ggucucaaag ccaaauuuaa cguucuaaca      780

gggauugaag cagaagacgu caaaacuugg acucuaacau ucaacgcaga gaacuucgau      840

gaagaaaugg ucaaacuuga aaguagccua gacgacaaug cacaccaaau aauugaauca      900

uuacaagagu uauauucugg cgucuuacuu gccgggauag uuccagaaaa ucaaagccuu      960

ucccaggcaa ugauaaccaa auaugacgac caucaaaaac accucaaaau guuaaaagca     1020

guucgagaag cacuugcacc ugaagaccgu cagagacuua aacaggcuua ugaucaauau     1080

guagacggcc aagaaaacac uaaagccuau ucaaaagagg auuuuuaugg ggauauaaca     1140

aaagcgcuca aaaauaaccc cgaucaccca auagugagcg aaauuaaaaa gcuaauagaa     1200

uuagaucaau uuaugccaaa acaacgcacu aaagacaaug gagcgauacc ucaucaacua     1260

caucaacaag aauuagauag aauaaucgaa aaccaacaac aauacuaccc guggcuagca     1320

gagcucaacc caaauagcaa aagacaaacu gucgccaaau acaaacuaga cgaauuagug     1380

gcauuucgug uaccauacua uguagguccc cuaauaacag ccgaacaaca acaacaaagu     1440

agugaugcga aauuugcuug gaugaucagg aaggcagaag gacaaauaac uccauggaau     1500

uucgaugaca aaguagaccg acaagccagu gcaaacgaau uuauaaaaag aaugacaaca     1560

acagauacuu aucucuuagc ugaagacguc cugcccaaac aaucgcucau auaucaacga     1620

uucgaagucc uaaacgaacu aaacggcuua aagaucgaug accaaccuau aaccacagaa     1680

cuaaagcaag cuauauucac agaucuuuuu augcagaaaa caucaguaac uguuaaaaac     1740

auucaagauu auuuaguauc cgagaaaaga uacgccucac gcccagcaau uacaggacuu     1800

agugacgaaa auaaauuuaa cucaagacua ucaacauauc augauuuaaa aacaauaguc     1860

ggagaugcag uagaugaugu agacaaacaa gcagaccuag aaaaaugcau cgaauggagc     1920

acaauauucg aagacggcaa aaucuacuca gcuaaacuua acgaaauuga uuggcucaca     1980

gaucaacaaa gaguucaacu agcagcaaaa agauacagag guuggggaag acuaagugca     2040

aaacuacuaa cacaaauagu uaaugcuaau ggucaaagaa uuauggacuu auuaugggac     2100

accacagaca auuuuaugag aaucguacau ucagaggacu uugauaaacu uaucacagaa     2160

gcaaaucaaa ugaugcuagc cgaaaacgac guacaagaug uaauaaacga ccuauacaca     2220

agcccacaaa auaagaaagc ucuacgacaa auccuuuuag uaguaaacga cauacaaaaa     2280

gccaugaaag gucaagcccc agaacggauu uugauagagu ucgcacgcga agacgaaguc     2340

aaccccagac uaagcguaca acgaaaaaga caaguagaac aaguauauca aaacauaucc     2400

aacgaacuau uaaauaauac cgagauccga aaugaauuaa aagaccuauc uaauuccgcu     2460

cuauccaaua cccgucuauu ucuuuauuuu augcaagggg gccgugauau guauacaggu     2520

gauagcuuaa auauagacag auuauccaca uaugacauug accacauacu accacagagc     2580

uucauaaaag auaacagccu ugauaaucgc guguuaguaa gccaacguau gaauagaagc     2640

aaggcagauc aaguaccuac ugauuucaca agcguagagc uaggccagaa aaugcaaaua     2700

caaugggaac aaauguuaag ggcuggacua auaacuaaaa agaaauauga caauuuaaca     2760

cuaaacccgg aucacauuag uaaauacgca augaaagguu ucaucaacag acaacugguc     2820

gaaacaagac agguaaucaa auuagccacu aaucuacuaa uggaacaaua cggagaagau     2880

aauauagagc ucaucacugu aaagagugga cuaacccacc aaaugcguac ugaguucgau     2940

uuccccaaaa accguaaucu gaauaaucau caccaugccu ucgaugcaua ccuaacagca     3000

uuuguuggac uauacuuacu uaaacgauau ccaaaacuaa aaccuuacuu cguuuauggu     3060

gaauaccaaa aagcguccca acaggauaaa uggcgaaauu uuaacuuuuu aaacggauug     3120

aagaaagaug aacuuguaga ugaaaacaca gaagcaguua uuuggaauaa agaaagcggg     3180

cuggcguauc uaaacaaaau auaucaauuu aagaaaauau uagugacaag agaaguacau     3240

gaaaauucgg gugcacuuuu caaucaaacc cucuacgcag ccaaagacga uaaagcaucc     3300

ggacaaggag guaaacaacu aauaccggcg aaacaggaca gaccuacugc auuauacgga     3360

ggauauagcg gaaaaacagu ugccuacaug ugcauuguaa gaaucaaaaa caagaaaggg     3420

gaccuauaca aaguaugcgg ugucgaaacu ucauggcugg cacaauuaaa acaacuaaca     3480

gacgaagacu cgaagaaagc cuuccuaaaa cagaaaauuu caccucaauu uaccaaagua     3540

aagaaacaaa aaggaaccau agugaaagua guagaagacu uugaagucau agcaccccac     3600

auucucauua aucagcgcuu cuuugauaac ggccaagaac uuacccuagg uucugccacg     3660

uauaaacaua acgaacaaga acugauacug gacaaaacag caguaaaauu acuaaacgga     3720

gccuuaccau uaacucaauc agaagaauug gcugaacaag uuuaugauga aauacucgac     3780

caaguuaugc auuauuuucc cuuauaugau acaaaucagu uccgggcaaa auuauccgca     3840

ggcaaagcug ccuuuaugaa auugccuugg aaaucccaau gggacgggaa caaaauggua     3900

cagguaggac aacaaguaau cuuagacaga guacucauug gauugcacgc aaaugcagcu     3960

gucucugacu uaggaguauu aaaaauaucu acuccauuag guaagaugca acaacccagu     4020

ggcauauccc uuucaccaga cacucaaaua auauaccaau caccaacugg auuauucgag     4080

agaagaguug cacuaagaga uuuauga                                         4107


<210>  5431
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5431
augaguaggc cauauaauau ugguuuagau auaggaacaa guucaauagg uuggucagua       60

gucgaugauc aaagcaagcu aguauccgua cguggaaaau acggcuaugg uguccgauua      120

uacgaugagg gacagaccgc ugcagaaaga agaucauuua gaacaacaag acgaagacua      180

aaaagaagaa aauggagacu cggccucuug cgagaaaucu uugaacccua caucacccca      240

gucgacgaua cuuucuuucu uaggcaaaaa cagagcaacc ucucacccaa agaccaaaga      300

aaauuauauc cccaaacuuc ucuauuuaau gaccgaacag aucgugcauu uuaugaugau      360

uaucccacaa ucuaccauuu acgauacaaa uugaugacug agaaaagaca auucgacauc      420

agagaaauau auuuagcaau gcaccauaua gucaaauacc guggacauuu ccuaaaugaa      480

gccccuguaa gcucauuuaa aucauccgag auaaauuuag uagcacauuu ugauagacuu      540

aauacuauau uugccgauuu auucucagaa ucaggcuucc aacuagaaac cgauaaauua      600

gcagaaguga aagccuuauu acucgauaau caacaaucug ccagcaacag acaaagacag      660

gcauuaucac uaauauauac ccccucuaca aacaaagcag uagagaaaca aaauaaagcc      720

auagcgacag aacuuuuaaa agccauacuc gggcugaaag caaaauucaa uguccuuaca      780

cgaauagaag cagaagaugu uaaagcaugg acauuaacuu uuaaugccga aaacuucgau      840

gaagaaaugg uuaaauuaga aagcucauua gaugacaaug cucaccaaau uaucgaauca      900

uuacaagaau uauacucagg aauccuacua gcgggaauag uucccgaaaa ccaauccuua      960

ucacaagcua ugaucaugaa auacgaugac caucaaaaac accugaaaau gcuaaaagca     1020

guaagagaag cauuagcgcc agaagaucga caacgacuca aacaagcaua cgaucaauau     1080

guagaugggc aagaaaauac aaaagcauau ucuaaagaag acuucuacgg cgauauuaca     1140

aaagcauuaa agaauaaucc agaccacccg auuguauccg aaauaaagaa auuaaucgaa     1200

cuagaccaau ucaugcccaa acagagaaca aaagauaaug gagcaauucc acaucaacug     1260

caccaacaag aacuggacag aauaauagaa aaucaacaac aguauuaccc augguuagca     1320

gaauugaacc caaauaguaa aagacagaca guggccaaau acaaacuuga ugaacucgua     1380

gcauucagag uaccauauua cguaggacca uuaauuaccg cugagcagca acggcaaagc     1440

ucagacgcaa aauucgcuug gcuuauaaga aaagcugagg gacgcaucac uccauggaau     1500

uucgacgaua aagucgaccg acaggcauca gcgaaugagu ucaucaaacg caugacuacc     1560

acagauaccu aucuacuagc agaagacguu cuaccaaaac agagccuuau auaucaacga     1620

uuugaagugc uaaaugaacu uaaugggcuu aaaaucgacg aucagccuau aaccacagaa     1680

cuaaaacaag cuauauucac agauuuauuc augcaaaaga caucaguauc ggucaaaaac     1740

auacaagacu accuuguaag cgagaaaagg uaugcaucac gcccagcuau aacaggucua     1800

ucagacgaaa acaaauuuaa cagucgccua agcaccuauc augaucuaaa agccauagua     1860

ggcgacgcug uagaagaugu ugauaaacaa guagaucuug aaaagugcgu ugaauggucu     1920

accaucuucg aagaugguaa aauauauuca gcaaagcuaa acgaaaucga cuggcuaaca     1980

gaucaacaac gaguacaauu agccgcaaaa cgcuaccgag gauggggccg uuugagugca     2040

aaauuacuaa cacaaaucgu aaaugcaaau ggacaacgua uuauggacuu acuuugggau     2100

acaacagaca auuucaugag gauaguccac agcgaagauu ucgacaaauu aauuacagaa     2160

gccaaccaaa ugaugcuagc ugaaaacgac guucaagaug uaauaaacga ucucuauaca     2220

agcccacaaa acaagaaagc ccuaagacaa auauugcuag uaguuaacga cauacaaaaa     2280

gccaugaaag gacaagcgcc ggaaaggaua cuaauugagu ucgcaagaga agacgaaguu     2340

aacccgagac uauccguaca acgaaaacga caaguagaac aaguauauca aaacauuagu     2400

aaugaacuuc uaaacaauac agaaauaaga aacgaauuaa aagaucuauc aaacucugcu     2460

cucucaaaca ccagacuauu ucuauacuuu augcaaggug gaagggacau guauaccgga     2520

gacagucuca acauagauag auuaagcaca uacgacauag accacauacu cccccaaucu     2580

uucauaaagg acaauucauu agacaaccgu guauuagucu cccagaaaau gaaucguuca     2640

aaggcagauc aagucccuac ggacuucacu ucgguugaau uaggaaagaa aaugcaacua     2700

caaugggaac aaauguuacg agcaggguua auaacuaaaa agaaauacga caaucuaaca     2760

cuuaauccug accacauuuc uaaguaugca augaaaggau uuauuaaccg acaacuugua     2820

gaaacccguc aaguaauaaa acucgcaaca aaucuucuca uggaacaaua uggagaagac     2880

aacauagaau uaaucacagu uaaaucaggc uuaacacauc aaaugagaac cgaguucgac     2940

uucccuaaaa acagaaauuu gaauaaucac caccacgcau uugaugcuua uuuaacugcu     3000

uucguagggu uguacuuacu aaaacgauac ccaaaguuaa aaccuuacuu uguauacgga     3060

gaauaucaaa aagcguccca acaagacaaa uggagaaauu ucaacuuucu aaacggucua     3120

aagaaagacg agcuaguaga cgaaaacacc gaagcuguaa uaugggacaa agaaucaggu     3180

uuagcauauu uaaacaaaau uuaucaauuc aagaaaauac ucguaacaag agaaguacac     3240

gaaaacucug gagcacuauu uaaucaaacg cucuacgccg ccaaagauga uaaagcaagu     3300

ggucaaggcg ggaaacaauu aauuccagcu aaacaagaca gaccgaccgc auuauauggc     3360

ggguacuccg gaaaaacagu agcauacaug ugcauaguaa gaauuaaaaa caagaaagga     3420

gaucuauaua aaguaugugg cguagaaacg ucauggcuag cacaacuaaa acagcuuacc     3480

gaugaggaca gccaaaaagc auuucuaaaa cagaaaauaa gcccacaauu cacuaaagug     3540

aagaaacaaa aagguaccau agucaaagua guagaagacu ucaaagucau agcaccgcau     3600

auacuaauaa accaaagauu cuuugauaau ggacaagaac uaacccucgg aagugcaacu     3660

uauaaacaua acgaacaaga acuuauauua gauaaaacag caguaaaacu guugaacggc     3720

gcacuuccau uaacacaauc cgaagaacua gcugagcaag uauaugacga aauauuagau     3780

caaguaaugc auuauuuucc ucuuuacgac accaaccaau uuagagcaaa guugucagca     3840

ggaaaagcag ccuucaugaa acugccuugg aaauccaucu gggacgguaa caaaaugaca     3900

cagauuggac aacagauaau ccuagaccgu auacuaaucg guuuacacgc gaacgcugcu     3960

guaagugacc uaggaguccu uaaaauaagu acuccauuag gaaaaaugca gcaacccagu     4020

ggaauuagcu uaucuccaga uacccaaaua auauaccaau cacccacugg auuauucgaa     4080

agacgcguag ccuugagaga uuuauga                                         4107


<210>  5432
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5432
augguugaaa agaaaagcua uucuauuggc uuagacauag gaacaaacuc cguaggaugg       60

gcaguaauaa ccgaugauua uaaagucccu gccaagaaaa ugaaaguucu uggaaacaca      120

uccagacaau caauuaagaa aaauaugaua ggagcucucc ucuuugauga agguggaccu      180

gcagcaucaa cucgaguaaa acggacaacu cggcgaagau auacuaggag gaaaaacaga      240

cuuuguuauu uaagagauau auuugagucu gagaugcaca cgaucgacaa acacuucuuu      300

uuacgccucg aagauucauu uuuacauaaa agcgauaaaa gauaugaagc acacccaauu      360

uuuggaacuc uacaagaaga aaaagcguac cacgauaacu acccaaccau uuaucacuua      420

agaaaagccc uagcugauaa uacugaaaaa gcagaccuac gcuuaauaua cuuagcauua      480

gcacacauua ucaaauuuag aggacauuuu cugaucgaag gagcauuaag cgcaaauaac      540

acugacgugc aacaauuggu ccaugcucua guagacgcuu acaauauaau guucgaagaa      600

gaccaauuag acauagaagc caucgacgua aaagcaaucu uaacagagaa aauaucuaaa      660

acgaggagau uagaaaaucu aauaucuaau auuccuggac aaaagaaaaa cggacuguuc      720

gguaaccuca uagcacuaag ucuaggauua acaccuaauu ucaaaaguca uuuuaaccuu      780

ccugaagacg cuaaacuaca auuagccaaa gauacauacg augaagaacu uaacaauuua      840

cuaacacaaa uaggcgauga auaugcagau uuguuccuaa gcgccaaaaa ccucagugac      900

gcaauucucu uaucggauau acuaacgguc aacggugacg gcacacaagc uccacuaucc      960

gcaucuuuaa uuaaaagaua cgaagaacac agacaagacu uagcccuacu aaaacaaaug     1020

uucaaagaac aacucccuga ccucuauaga gacguauuua ccgacgaaaa uaaagaugga     1080

uacgcagguu auauaagcgg aaaaacaagu caagaagcau ucuacaaaua uaucaaacca     1140

auuuuagaaa cccuagacgg agccgaagac uucuuaacca aaauuaaccg ugaagacuuu     1200

uuacgaaaac aacgaacuuu cgacaaugga aguauaccac accaaaucca ccuaggagaa     1260

cuccaagcua uauuggaacg ccagcaagcc uauuacccau uucucaaaga uaaccaagag     1320

aaaauagaga aaauacugac cuuccgaaua ccauacuaua uuggaccccu cgcaagaggc     1380

aacagcaggu ucgccuggcu aacaagaaca ucagaccaga aaaucacucc auggaauuuc     1440

gacgaaaugg uagaucaaga agccucagcg caggcuuuua uugaaagaau gaccaauuuu     1500

gacgaauauu uaccacaaga aaaaguacuc ccaaaacacu cacuaacaua ugaauacuuc     1560

acaguauaca augaacuaac aaaaguaaaa uauguuacug aaggaaugac caaaccagaa     1620

uuuuuauccg cagggcaaaa agaacaaaua guagaguuac ucuucaagaa auaccgcaaa     1680

guaacaguua aacaacuaaa agaagacuuc uuuuccaaaa ucgaaugcuu ugauaccgua     1740

gauauaucag gaguagaaga uaaauuuaac gcgucacugg gaacauacca cgauuuauua     1800

aaaauuauca aagauaaagc auucuuagac aacucagaaa augaaaauau aauagaagac     1860

auuauauuaa cacucaccuu guucgaggau aaagaaauga uagccaacag acucgcaguc     1920

uacgaggauu uauuugacca aaacguucua aaacaauuaa aacgucgcca uuacaccggc     1980

uggggacgac uaucaaaaca auuaauaaac ggcaugagag acaaacauac aggcaaaaca     2040

auauuagacu ucuugaaagc ggacggauuc auaaaucgaa acuuuaugca auuaauaaac     2100

gaugauaauc uaucuuuuaa agaagagauc aagaaagcac aagaaggggg auuaaaagac     2160

ucuauaaaug accaaauaag agaccucgcu ggaucuccug cuauuaagaa agggaucuua     2220

caaacaauua acauagucga cgagauuguc aaaauaaugg gaaaagcacc ccaacauauc     2280

guaguagaaa uggcucgaga cgugcagaaa accgacauag gaguaaaaca aucuagagaa     2340

cgaaugaaaa gaguacaaga aguacucaag aaauuaggaa gucaacugcu aaaagaacac     2400

ccaguagaaa acuuucaauu acaaaacgaa agacuauauc uauacuaccu ucaaaaugga     2460

aaagauaugu acacaggaga agaauuaucu auaucaaauu uaucgcacua ugauauagac     2520

cauauaaucc cacgcagcuu cauaaaagac gacucuauug auaacaaagu acuaacucgc     2580

ucagaacaca aucgaggaaa aaccgacaau guacccagua ucgaaguugu aaaaagaaug     2640

aaaccauacu ggcaaaaauu gcuagacaca aaaguaauau cacaacgaaa auuugacaac     2700

cuaaccaaag cagaacgcgg uggcuuacaa gaaucagaca aagcuaacuu uauccagcgc     2760

cagcuaguag aaacaagaca aauuaccaaa cacguagccc aaauucuaga uaguagauuc     2820

aauacagaaa gagacgaaaa agauagacca auaaggagag uaaaagucau uacccucaaa     2880

aguaaauucg uaucagauuu ucgacaagac uucggcuuuu auaaacuccg agagauaaac     2940

gacuaccauc augcacacga ugcauaucuc aacgcaguug uaggcacagc gcuacuaaaa     3000

auguauccca aacuagccuc agaauuugua uauggggacu accaaaaaua ugaucuaaaa     3060

cgcaugguag guaaaagcgg caaagcaagc ggccacgcaa cagcaaagua cuucuuuuau     3120

ucuaauuuaa ugaacuucuu uaaaagugaa guaaaacuag caaauggaaa cauaauaaaa     3180

cguagcccca ucgaaguaaa ugaagaaacu ggcgaaauag uaugggauaa aaccaaagau     3240

uucggcacug ucagaaaggu acugucagca ccccaaguaa acauugucaa gaaaacugaa     3300

auacaaacag gagguuuuag caaugaaacu auacuaagca aaggaaaauc aagcaaauua     3360

auaccgagaa agaauaaaug gcgagacacu acaaaguaug gaggauucaa uacacccacc     3420

gucgccuacu cuguauuagu cguagcuaaa guagaaaaag gcaaagccaa gaaacuuaaa     3480

ccuguaaaag agcuaguugg uauuacuaua auggaacgca caaaauuuga agcaaaucca     3540

auagcauuuc uagaaagcaa agguuaucau gacauccaag aacaucuaau gaucacacuc     3600

ccaaaauauu cauuguucga acuagaaaau gguagaagac gacuacuugc aucagcuacc     3660

gaacuacaaa aagguaacga gaugguacua ccgcaacauu uagucaccuu cuuauacagg     3720

guaucaaaac gagacaaagg uacccaaagu gaaaauaugg aauauaucag uaaccauaaa     3780

gaaaaauuua ucgaaauauu ucauuauaua auacgauacg cagagaaaaa ugugauuaaa     3840

ccaaaaguaa uugagagauu aaacgauaca uuuaaucaaa aauucaauga cagugaucuc     3900

acagaacuau caauauccuu ucuuaacuua uucaaauuca caucguucgg ggcaccagaa     3960

aaauuuacgu uucuaaauuc agaaaucaaa caggacgaug ucagauauag aucaacaaaa     4020

gaaugccuga acagcacauu gauucaucaa agcgucacug gcuuauauga aacaagaauc     4080

gauuuaucac aauuuggggg agauuga                                         4107


<210>  5433
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5433
auggauaaaa aguacaguau uggacucgau auugggacaa acucaguagg augggcaguc       60

auaacagacg acuauaaagu accaucuaaa aaguucaaag ugcuuggaaa cacggacaga      120

cacagcauca agaaaaaucu aauaggcgca uuauuauuug auucuggaga gacagcugaa      180

gcaacaaggu uaaaacgaac agcuagacga cgauacacac ggaggaaaaa cagaauuugu      240

uaccuacaag agauauuuuc gaaugaaaua gcaaaaguag augauuccuu cuuucaccga      300

cuagaagaau cauuccuagu ugaagaagau aagaaacaug aaagacaucc uaucuucgga      360

aacauaguag augaaguagc auaucaugag aaauacccaa cuauuuauca ucuaagaaag      420

aaauuagcag auaguacgga caaagcggac uuaagacuaa uauauuuagc auuagcacau      480

augaucaaau ucagaggaca cuuccuaauc gaaggagauc uuaaucccga caacucagau      540

guggacaaac uguucauaca gcuagugcaa acguauaauc aauuauuuga agaaaauccu      600

auaaacgcaa gucguguaga ugccaaagca auacuaucug cuagacuauc gaaaucaaga      660

agacucgaaa aucuaauugc acaacuacca ggcgagaaaa ggaacggcuu auucgguaau      720

cugauagcau uaagccuagg acuaacacca aacuucaaau caaacuucga ucuagccgaa      780

gaugcaaaac uacaacuaag caaagacaca uacgaugaug acuuggacaa uuuacuagcu      840

caaaucggcg aucaauacgc agaccuguuu cucgcagcua aaaaucuguc cgacgccauc      900

cuacuaucag auauuuuacg agucaauaca gagauaacaa aagcuccacu cucggcuuca      960

augauuaaaa gauaugauga acaucaccaa gaucuaaccc uacucaaagc ccuaguacga     1020

caacaacuac cagagaaaua uaaagaaaua uuuuucgauc aaaguaaaaa uggauaugcu     1080

ggauauauag augguggagc aucacaagag gaguucuaua aauuuauaaa accuauuuua     1140

gagaaaaugg auggaacuga agaauuauua gcaaaacuua aucgagaaga ccuacuccgg     1200

aaacaacgaa cauuugacaa cggaucuaua ccucaccaaa uacacuuagg agaacuacac     1260

gcaauucuuc gucgccaaga agacuuuuau ccuuuucuua aagacaauag agagaaaauu     1320

gagaaaauac ugacauucag gauaccauac uacguagguc cucucgcaag gggcaacagu     1380

agauucgcuu ggaugacaag gaaaucagaa gaaacuauaa caccauggaa uuucgaagaa     1440

gucguagaca aaggcgcuag cgcucaaucg uucauagaaa gaaugacgaa uuuugauaaa     1500

aaucugccaa acgaaaaagu gcucccuaaa cauagccucc uauacgaaua uuuuaccguu     1560

uauaacgaac uuacaaaagu aaaauaugua acagaaggua ugagaaaacc agcauuuuua     1620

ucuggagaac aaaagaaagc gauaguagau cuauuauuua aaacgaaucg caaaguaaca     1680

gucaaacaac uaaaagaaga uuauuucaag aaaauagaau guuuugauag uguagaaaua     1740

ucagggguag aagaccgguu caaugcuagc uuaggaacuu accacgacuu guuaaaaauu     1800

auuaaagaua aagacuuccu ggauaacgaa gaaaacgaag acauacuuga ggacauaguc     1860

cuaaccuuga cacuguucga agauagagaa augauagagg agagacuaaa aacauaugca     1920

caccuauuug acgacaaagu gaugaaacaa cucaaaagaa gacgauacac cggauggggc     1980

aggcuaucaa gaaaacuaau uaauggaaua agagacaaac aaucugguaa aacaauucug     2040

gauuuccuaa aaagcgacgg auuugcaaau agaaacuuua ugcaguuaau acaugacgac     2100

agccucacuu ucaaagaaga ucugcaaaaa gcacaaguua gcggccaagg ggacagucua     2160

cacgaacaua uagcaaacuu ggccggauca cccgcaauua agaaagggau acugcaaacc     2220

gucaaaguag uagaugaauu aguaaaagua augggacgac auaaaccaga aaacauugua     2280

auagaaaugg cucgagagaa ccagacgacc caaaaagguc agaaaaauag uagagaaaga     2340

augaaaagga uagaagaagg gauaaaagag cuuggaagug acauacuuaa agaauacccc     2400

gucgaaacua cacaacuaca aaacgaaaaa cuuuaccucu acuaccuaca aaacggccgu     2460

gauauguaug uagaucaaga acuagauaua aauagauuau cagauuauga cguggaccau     2520

auaguaccac aaucauuuuu aaaagaugac agcauugaua acaagguccu uaccagaagc     2580

gacaaaaauc guggaaaauc agauaacguc ccgagcgaag agguuguaaa gaaaaugaaa     2640

aacuacugga aacaacuacu caaugcuaaa uuaauaacac agcgaaaauu ugacaauuua     2700

acaaaagcag aacgcggugg ccuaagcgaa cuagacaaag caggauuuau caaacgacag     2760

cuaguugaaa ccaggcaaau cacaaaacac guugcccaaa uacuagauag ccgaaugaau     2820

acaaaauaug acgaaaacga caaauuaauu cgagaaguua gaguuauaac ccugaaaucu     2880

aaacuuguua gcgauuuucg aaaagauuuc caauucuaua aaguaagaga aauaaacaac     2940

uaucaccaug cacaugaugc auaccuaaac gcuguaguug gcacugcacu gauaaagaaa     3000

uauccaaaac uugaaaguga auuuguauau ggagauuaca aaguuuacga uauacguaaa     3060

augauagcaa aaagugaaca agaaaucgga aaagcgacag caaaguacuu uuucuauagu     3120

aauauaauga acuuuuucaa gacagaaaua acauuagcca acggagaaau acgaaaaaga     3180

ccucuaaucg aaaccaacgg agaaacaggg gagauaguau gggauaaagg cagagacuuc     3240

gcuacugucc gaaagguacu uucuaugcca caagucaaca uagucaagaa aacagaagua     3300

caaacaggag gcuuuucuaa agaaucaaua uuaccaaaac gaaacucgga uaaauuaauu     3360

gcaagaaaga aagauuggga ucccaagaaa uauggaggau uugauagccc gacgguagca     3420

uacagcguau uagucguagc caaaguagaa aaagggaaau caaagaaauu aaaauccguc     3480

aaagagcucg uaggcauuac aauaauggaa cgcucaaguu ucgaaaaaga uccgguugac     3540

uuuuuggagg ccaaaggaua caaagaggua cgaaaagauc uuauuauuaa auuaccaaaa     3600

uacaguuuau ucgaacuaga aaauggacga aaaagaaugu uagcuagugc aggcgaacuu     3660

caaaaaggaa acgaacucgc acucccgagu aaauauguua acuuccuaua uuuagcauca     3720

cacuaugaaa aauugaaagg gaguccagaa gacaaugagc aaaaacaacu auuuguagaa     3780

caacacaaac auuaucuaga cgaaauuaua gaacaaauaa gugaguucag caaaagagua     3840

auauuagcag acgcaaacuu agacaaagua uuaucggccu auaacaaaca cagagauaaa     3900

ccaauacgug aacaagcuga aaacauaaua caucuuuuua cacuaacgaa uuuaggagca     3960

cccgcagcuu uuaaauguuu cgacacaaca aucggaagaa acagauacaa aaguaucaaa     4020

gaaguacuag acgcaacacu cauucaucaa uccauuacag gcuuauauga aacaagaauc     4080

gaucuuuccc aauuaggagg ugauuga                                         4107


<210>  5434
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5434
auggauaaga aauauuccau uggguuagau auaggaacaa guucaauagg auuugucgga       60

guagacgaaa auggguggcc uuuaaacaua aaaggcaaaa gggcaauagg agcgcauuug      120

uucagagaag gcgaagcagc ugcagaaacu cgagguuucc gaaccacccg aagacgcuac      180

aaccgaagga aauggagacu aagauuauua agggaaauau ucgacgaacc aaucucaaga      240

gucgacccca cguuuuucgc aaggaugaaa gaaaguucug uaucaccgaa agauccaaac      300

uacgacggau uagcaaaaga aauauugauc ccaagagaua aagacggcaa aauauuauau      360

cccacuauau aucaccuaag gcacgcauua auguaugaag accaccaauu ugacauaaga      420

gaaauauauc ucgcaauaca ccacauugua aaauaccgag gacauuuccu acuagaugga      480

gccgcuaaaa auuucgaauc gucaaauuua gaccuuaaau ccgauauaca cgaaauuaac      540

gcaauauuag ucgguauauu uggaaaugaa caaguccaau uuaaugacca auacguagaa      600

aaacugaaag aaguguuauu agauucaacc caaagucgaa gugauagaca aaaagccgca      660

gcaaaacugc ucuuacccac gaaugacuua gacaaagaua ccacaaaaau cuuaaaaaca      720

gucucuacag aaauuacaaa agcaauuacg ggacuaaaag ccaaauucuu uguuaucaca      780

ggcaaugaua ucgcgaaaga ccaacaaaaa gacuggacgu ucucgaucga uacauuagac      840

gagaaaaugu ccguaauaga acccucguuc ucagagaaaa auuuuagccu gauaacaauu      900

uuacaaaaac ucuacucuuc uguaacccuu gccggaauag uccccaacgg acuaucuuua      960

agugaagcaa ugauucaaaa auacgaccgc cacgcaagcg auuuaguacu cuugaagaaa     1020

uauugggaua ccaaacccaa aguacucaaa caccaacucc aaaacacuua ugccgaauac     1080

aucgaaauac accaaguagu agaugaguuc uauagugacc ugagcaaacu aauuaaaaac     1140

gaugaugaca cacucgcuca agaaauaaaa ucaaaaauaa caauaggcaa cuucuuaccc     1200

aaacaacgua cuacagaaaa uggugucaua ccauaccaac uacaacaacg ggaauuagac     1260

accaucauca aaaaccaaca agaauauuac ccauggcuag cagaagaaaa cccaguugcu     1320

gaccaccaaa ucgcauuccc cuauaaacua gacgaacucg ucggauuucg cauaccguau     1380

uacguagggc ccaugaucgc aaaagaagau acaucaaaca gcaaaaacag caaauuugca     1440

uggaugauaa gaaaacaauc aggcgaaguc acaccaugga auuucgacga gaaaauagac     1500

cgcacugcga gugcuacagc auuuauagac agaaugacca cgaccgauac auaucugaua     1560

ggagaagacg uacuuccaaa acaaucucuc auauaccaac gauuugaagu ccugaacgaa     1620

cuaaacaaaa uaucuauuga uggccaacca auuacacgaa aacaaaaaca aagugucuac     1680

acucaugucu uugagaaaaa gaaaacugua caaguaaaag acguccaaaa auucuuagua     1740

ucuucaggag aaguaaugag uuuacccaaa auuggagggc uggcaaauga aaaacaauuc     1800

aacuccaguc uaaguacgcu auacgauuac aacaaaauaa uacccgaugc auuagaagac     1860

agaaacaaag uagaugauau agagaaaaua auacucuggu caacaauuuu ugaagacaac     1920

aguaucuucg uugaaaaacu ggauucuauc gacuggcuca cuagcagcca acggaaacgu     1980

uuagcacaaa aacgauauag gggauggggc cgacuaaguc uaagauuauu aacaacauuc     2040

aaaaacaaag auggauacuc aauccucgac gaauuaugga acucgaauca caacuuuaug     2100

gaaauacaag cacaaucuga cuuuucagaa cagauugcca cugccaacaa caacaaccua     2160

ucccaaguuc uaccagcaaa augggaacca ggacgcaaaa accuagcuuc ccuagacgaa     2220

uuuauaucua accucuauac aucaccacag aauaagaaag cauuaagaaa agcgauaaga     2280

gucguacagg acaugaugac cgcugccggg cacaugccag agaaaauauu caucgaagug     2340

gccagagaua cguccaauuc acaccaacaa acaauagcaa gacaaagaca guugcaacaa     2400

uuauaccaau cagaagacaa agaagucaua gauucaucag uaagauccga acuagacgac     2460

auacuaaaga acagagcaaa caucaaagau caacuauucu uauacuucca acaaggagga     2520

cgagauuuau acacuggaaa agcaaucaau aucgauaaau uaagucuaua ccacauagac     2580

cacauacuuc cucaaagccu agucaaagau gauagucuag acaaucgagu ucuaguuuca     2640

cagaaaacaa augaagacaa aggcguaacc uucgugagug acaaauacau gaauaacaug     2700

cugccuacgu ggaaaaggug gcaugaaaug ggacuuauau cugccagaaa acugaaaaac     2760

uuaaccaugc ggccugauga aaucgacaaa uaugcaccag gauucauaca acgacaauua     2820

guggaaacac gccaaguaac caaauuaguu aucgaacuuc ucgaugcccu auuaggaaau     2880

agccaaaccg aaauuauuac aauaaaagcc ucacuaaguc accaauuuag acaacaauuc     2940

gacuuaguaa aaauuagaga agugaacgau aaccaccacg ccuuugaugc auucuuggca     3000

gcaagaauug gaacauacuu auauaaaagg uauccuaaac ucagaccaua uuuuguguac     3060

ggacaauaca ugcaaaacca caaaaacuua aaauccauua aucugauaaa aacauuagau     3120

uccgauaaag caauagucga ugaaggcaca ggagaaaucc ugugggacaa gaaaucagac     3180

uuagcaaaaa ugaucaaaau auacaauuau caacgacuuc ucauaacaca ugaaucaaga     3240

acauccuuug gagcaaugua uaaacagaaa auacuaaaag caacagacgc aaagcagaac     3300

ggagguggau ucaaauuagu cccaacgaaa agaaacagac ccguagauau cuaugggggc     3360

uacacaggcc aaacagcagc uuaccuaaca aucguacgcg uauacucuaa gaaaacuucc     3420

gaauuaagag uauugcccau auacaccaac aaaguccaac aaauagaaaa agcaaugacu     3480

caaggggaca aagcuacaau guuagccuuu aagaaaguca ucgcaccgca auuaggaaaa     3540

gagaauaaga aaacccacga aauaaaaauc cccgaauuug acguuguaau ccccaaagua     3600

uuguuagacc aacugguaau ugacgauaua gccggaagcc cucacagauu cagauuagga     3660

acagauacau acuaccacaa cucucaacaa cuuuuucuuc cacuaucuau gcaacgucaa     3720

cuaauggauc gagaaucaga cgacaaagug uaccuagaca ucuucaacga uguccuaaaa     3780

caaguugacu ccuacuuuaa cuuauacgau guaaacggcu uucgcgcagc acuucaaaaa     3840

ucuaagaaag cauucgaaca auuaccaaua aaugauuuuc cuaaagacgu cccagacaau     3900

aaaaagaaga aaggaaaaag gagcauccua aaagaaauuu uuauuggauu acaugcaaac     3960

gccacgcuag gaaaccuaaa acaaauggga auaaaaacgg auuucggcaa gcuccaaaaa     4020

ucaaacgguu uaagaauaac agaaaacugc aaaauuauuc accagucccc cacaggacua     4080

uuugaaagaa uagucccaau aaaauga                                         4107


<210>  5435
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5435
auggauaaga aauauagcau uggccucgau auagguacua acagcguagg uugggccguu       60

auuacagacg auuacaaagu accaucgaag aaauucaaag uucuugguaa uacagauaga      120

cauucaauaa agaaaaacuu aauaggagca cuguuauucg auucaggcga aauugccgaa      180

gcaacuagac ucaaaagaac agcgagaagg agauauaccc gacgcaaaaa ccgaauaugc      240

uaucuacagg aaauauuuuc uaacgagaug gccaaagugg acgacuccuu uuuccauaga      300

cucgaagaaa guuucuuagu cgaagaagac aagaaacacg aaagacaccc aauauucggg      360

aauauagugg augaaguagc uuaucacgag aaauauccua cgauauauca ccuaagaaag      420

aaauuaguag auuccaccga caaagcagac uuacgacuaa uuuaccuagc gcucgcacau      480

augauuaaau uccgcgggca cuuccugaua gaaggagacu ugaauccaga uaauucggau      540

guugacaaac uauucauuca acucguucaa acauacaauc aacuuuucga agaaaacccc      600

auaaaugcau caggaguaga cgccaaagca aucuugagcg cgagacuauc caaaucaaga      660

agacuggaaa acuuaauagc ccaguugcca ggugaaaaga aaaauggucu auuugggaau      720

cuaaucgcac uaagccuagg uuuaacucca aauuucaaau caaauuucga ucuugcagaa      780

gacgcaaaac uacaacuaag uaaagacacu uaugaugaug accuugauaa ccuacucgca      840

cagauaggug accaauaugc cgacuuauuu uuagcagcga aaaauuuaag ugacgcuauc      900

cuguuaagcg auauauuacg acuaaacagc gaaauaacaa aagccccacu uucagcaucu      960

augauaaaaa gauacgacga acaucaucaa gauuuaacac uacuaaaagc acuuguacgc     1020

caacaauuac cugagaaaua uaaagaaauc uucuucgauc aaucgaaaaa uggcuaugcu     1080

ggauauauug auggaggggc cucacaagaa gaguucuaua aauuuauaaa accaauacuu     1140

gagaaaaugg acgguaccga agaacugcua gcuaaauuaa auagagaaga ucugcucaga     1200

aaacaacgua ccuucgacaa ugguuccaua ccucaccaaa uccacuuagg ugaauuacac     1260

gcgauacuca gaagacagga agacuucuac ccauuucuca aagacaaccg agagaaaaua     1320

gagaaaauac ucaccuucag aauacccuac uauguuggac cauuagcaag aggaaauucu     1380

agauucgcgu ggaugacacg aaaaucugaa gaaacgauaa caccuuggaa cuucgaagaa     1440

guuguagaua aaggagcuuc agcucaaucc uucauagaaa gaaugacaaa cuucgauaaa     1500

aauuugccaa acgaaaaagu ucuccccaaa cacagcuuac uauacgaaua uuucacagua     1560

uauaaugaac ucaccaaagu aaaauacguc acagaaggaa ugcgaaaacc ggcauuucuc     1620

uccggagaac agaagaaagc cauuguugac uuauuauuua aaacgaacag aaaaguaaca     1680

guuaaacaac uaaaagaaga cuacuuuaag aaaauagaau guuuugauuc ugucgaaauc     1740

uccgguguag aagaccgauu uaaugcaagc cuagguacau aucacgauuu acucaaaaua     1800

auuaaagaua aagacuuuuu agacaacgaa gaaaaugaag acauccuaga agacauagua     1860

uugacccuca cauuauucga agacagagaa augaucgaag aacgauuaaa gaccuacgca     1920

cauuuauucg augauaaagu uaugaaacag cugaaacgcc gcagauacac aguaugggga     1980

agacuaucgc gaaaacucau aaauggaauu agggacaaac aaaguggaaa aaccauccua     2040

gauuuccuca agucggaugg auuugccaau cgaaacuuua ugcaacucau acaugacgac     2100

agccuuaccu ucaaagaaga uauacaaaag gcacaaguaa guggucaagg agauucguua     2160

caugaacaua uagcuaacuu agcugguucg ccggcaauaa agaaaggaau acuccaaacc     2220

guaaaaguag uagaugaauu gguaaaagua augggaaggc acaaaccaga aaauauagua     2280

auugaaaugg ccagagaaaa ccaaacaacu caaaaagggc agaaaaauag ucgagaaagg     2340

augaaacgaa uagaagaagg aauaaaagaa cucggcaguc aaauucuaaa agaacauccc     2400

guagaaaaca cccaacuaca aaaugaaaaa cuauacuuau auuauuuaca aaauggccgc     2460

gacauguaug uagaucaaga auuagauaua aaccgacuga gcgauuauga cgucgaccac     2520

auuguuccac aaucguuuau aaaagacgac ucaaucgaca auaaaguacu aacaagaagc     2580

gauaaaaaca gagguaaauc cgacaacgua ccuucagaag aaguagugaa gaaaaugaaa     2640

aauuauugga gacaauuauu aaacgcuaaa cuaauaacuc aacgaaaauu ugacaaucuu     2700

acaaaagcgg aaagaggcgg ucucuccgaa cuagauaaag cagguuucau uaaaagacaa     2760

uugguugaaa cgcggcaaau aacuaaacac guugcacaaa ucuuagacuc aaggaugaau     2820

acaaaauaug acgaaaacga caaacucauu agagaaguaa aaguuauuac acuaaaaagc     2880

aagcuaguaa gcgacuuccg aaaagauuuu caauuuuaca agguuagaga aauuaauaac     2940

uaccaccacg cacaugacgc auaucuuaau gcaguagucg gaaccgcacu uauaaagaaa     3000

uacccgaaau uagaauccga guucguauau ggagacuaua aaguguauga cguuagaaaa     3060

augcuagcaa aaucagaaca agaaauuggc aaagcaacgg cuaaauacuu cuuuuauuca     3120

aauauaauga acuuuuucaa aacagaaaua acucuagcaa auggagaaau cagaaaacgu     3180

ccauugauag aaacaaaugg agaaacggga gaaauaguau gggauaaagg ucgagauuuc     3240

gcaaccguua gaaaaguguu aucaaugcca caaguaaaua uaguuaagaa aacagaagua     3300

caaacaggag gcuucucuaa agaaucaaua cugccaaaaa gaaacucgga caaauuaaua     3360

gcucgcaaga aagauuggga ccccaagaaa uacggaggau uugacucacc caccguagcu     3420

uacuccgugc uaguaguagc aaaaguagaa aaaggcaaaa guaagaaauu aaaaagcguc     3480

aaagaacucc uaggaauaac aauaauggaa agauccucau ucgagaaaaa ccccauugau     3540

uuccuugaag ccaaaggaua caaagaagua agaaaagauu uaauaaucaa acuaccaaaa     3600

uauagcuuau ucgaacuaga aaauggacga aaacgcaugc uggccagcgc aggggagcuc     3660

caaaaaggga acgaacuagc acuuccuagu aaauauguaa acuuccucua ccuugcaucu     3720

cauuaugaaa aacucaaagg uaguccagaa gacaacgaac aaaaacaauu auuuguugaa     3780

cagcauaagc acuaucucga cgagauaaua gagcaaauau cagaguuuuc aaaaagagua     3840

aucuuagcag acgcgaauuu agauaaaguc cucagugcau auaauaaaca cagagacaaa     3900

ccaauuagag aacaggcaga aaacauuauc caccucuuua ccuuaacaaa cuuaggggcg     3960

ccaacugcau ucaaauacuu cgauacaaca aucgaccgca aaagguauac cagcacuaaa     4020

gaaguacuug acgcaaccuu uauccaccaa ucaauuacag gacuauacga aacacgaaua     4080

gauuuaucac aauuaggcgg agacuga                                         4107


<210>  5436
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5436
auggacaaga aauauaguau aggacuagac auaggaacaa acucaguagg augggcgguu       60

aucacagacg auuacaaagu cccuucaaag aaauuuaaag uauuaggaaa uacugaucga      120

cauucaauua agaaaaauuu aauaggggcc cuauuauuug auagcggaga aacugcugaa      180

gccacaaggc uuaaaagaac ugcacggaga agguauacca gaagaaagaa ucguauuugu      240

uaucuacaag aaauuuucuc uaacgaaaug gcaaaaguug acgauucauu uuuccaucga      300

uuagaagaaa guuuccuugu agaagaggau aagaaacacg aaagacaccc aaucuucggu      360

aauauaguag augaaguugc uuaccacgaa aaauauccaa caauauauca ccuaagaaag      420

aaauuggugg auucuacaga uaaagcugau cucaggcuaa uauaccuagc uuuagcucac      480

augauaaaau uuagggguca cuuccuuauu gaaggagacc uaaauccuga uaauucagau      540

guagauaaac uauuuauaca auuagugcaa acauacaauc aacuauuuga agaaaacccc      600

auaaacgcuu caggggucga ugcuaaagca auacucagcg cacgacuaag uaaaucacga      660

agacuagaaa auuuaaucgc ccaauuaccc ggggaaaaga aaaacggacu cuucggaaac      720

uuaaucgcuc uuucacuagg acuuacacca aauuucaaau caaauuucga ccucgcggaa      780

gaugcuaaac uccaacucuc aaaagacaca uaugaugaug accucgauaa uuuauuggca      840

caaauaggag aucaauacgc cgaccucuuu uuagcagcua aaaaccucuc cgaugcaauu      900

cuguuaagcg acauacuucg acuuaauuca gagaucacca aagcaccuuu aucugcaucc      960

augauuaaac gauaugacga acaccaucaa gaucuaacau uacugaaagc cuuaguaaga     1020

caacaacuac cagaaaaaua caaagaaaua uuuuucgacc aaagcaaaaa uggauacgca     1080

ggguacauug auggcggagc gucucaagag gaguucuaua aauucaucaa accaauauua     1140

gagaaaaugg acgggacaga agaacuucuc gcaaaauuga acagagaaga cuuacuacgg     1200

aaacaacgua cauucgauaa uggaaguaua ccacaccaaa uucaucuggg agaauugcac     1260

gcuauccuuc guagacagga agacuuuuau ccauuccuaa aagauaauag ggagaaaaua     1320

gagaaaauau uaacauucag gauaccuuau uaugugggac cauuagcacg aggaaacuca     1380

cgguucgcau ggaugacccg uaaaagugaa gaaaccauaa caccuuggaa cuucgaagaa     1440

gucgucgaua aaggcgcaag cgcacagagc uucauagaaa gaaugacaaa uuuugacaaa     1500

aaucucccua acgaaaaagu auugccaaag cacucauuau uauacgaaua uuucacugug     1560

uauaacgaac uaacaaaagu uaaauacgua acggaaggaa ugagaaaacc agcauuccuc     1620

ucaggcgaac aaaagaaagc gauagucgau uugcuuuuca aaacaaauag aaaagucacg     1680

guaaaacaac uuaaagagga uuacuuuaag aaaauugagu gcuuugacuc cguagaaaua     1740

aguggugucg aagaucguuu uaacgcgagu cuagguacau accacgaucu ccuaaaaaua     1800

auaaaagaua aagacuuuuu ggauaacgaa gagaaugaag auauacucga agauaucguc     1860

cuuacauuaa cacuguuuga agaccgggaa augaucgaag aaagacuaaa aacauaugca     1920

cauuuauuug acgacaaggu aaugaagcaa cuaaagagaa gaagauacac gggaugggga     1980

agauuaagua ggaaacuuau aaaugggaua agagauaaac aaucuggaaa aaccauauua     2040

gacuuuuuaa aaucugaugg auuugcgaac aggaacuuca ugcaacucau acaugaugau     2100

ucccucaccu ucaaagaaga uauccaaaaa gcacaaguaa gcggucaagg cgacagccua     2160

caugaacaua uagcaaauuu agcgggauca ccggccauaa agaaaggaau acuccaaaca     2220

guaaaagucg uugacgagcu aguaaaaguu auggguagac acaaaccaga aaacauaguc     2280

auagaaaugg cacgagaaaa ucagacaaca caaaaaggcc agaaaaauuc uagagaaaga     2340

augaaaagaa ucgaagaggg gauaaaagaa uugggaucuc aaauacuuaa ggaacaucca     2400

guggaaaaua cacaauuaca aaacgaaaaa uuauacuuau acuaucuaca gaacggacga     2460

gauauguaug ucgaucaaga gcuggacaua aaccgucuau cagauuauga uguugaccau     2520

auuguacccc aaaguuuuau aaaagaugau agcauugaua acaaaguauu aacuaggagc     2580

gacaaaaauc gaggaaaauc gaacaacgua cccuccgaag aagucgugaa gaaaaugaaa     2640

aauuacugga gacaacugcu aaacgcaaaa cuaauaaccc aaagaaaauu cgauaacuug     2700

acgaaagcug aaagaggugg auuaucugaa cuugacaaag ccggauuuau aaaaagacaa     2760

cugguugaga ccagacaaau aacaaaacac gucgcacaaa uuuuagacuc aaggaugaau     2820

acuaaauaug acgaaaauga uaaauuaauu agggaaguaa agguuauaac acucaaaagu     2880

aaacugguca gugacuucag gaaagauuuc caauuuuaca aaguacggga aauaaauaau     2940

uaucaccaug cccaugacgc uuauuugaac gcaguaguag guacugcauu gauaaagaaa     3000

uaucccaaac ucgaaucaga guucgucuau ggagauuaca aaguauacga ugugagaaaa     3060

augauagcaa aaucugaaca ggaaauaggc aaagcaacag ccaaauacuu uuucuauuca     3120

aauauuauga acuucuucaa aacagaaauc acacuagcca acggcgaaau aagaaaacga     3180

ccauugauag aaacgaacgg cgaaacagga gagauaguuu gggacaaagg gcgugacuuc     3240

gcaacgguaa gaaagguacu uucaaugccu caaguaaaca ucguaaagaa aacagaagua     3300

caaacgggag guuucaguaa agaaucaauc cugccaaaaa gaaacaguga caaacuaauc     3360

gcucgcaaga aagacuggga ucccaagaaa uauggaggau uugacucacc aacaguggcc     3420

uauagcguau ugguuguagc aaaaguggaa aaaggcaaaa guaagaaacu caaaucugua     3480

aaagaacuac uaggaauaac uauaauggaa aggucuuccu ucgagaaaaa uccgauagau     3540

uuccuggaag caaaagguua uaaagaaguu cguaaagacu uaauagucaa auuaccaaaa     3600

uauagucuau uugaacuaga aaacggaagg aaaagaaugu uagcgucagc aggcgaacuc     3660

caaaaaggaa acgaauuagc acucccgagc aaauauguaa auuucuuaua uuuagcauca     3720

cacuaugaaa aacuuaaagg cagcccagag gacaacgaac aaaaacaacu auuuguugaa     3780

caacacaaac acuaucuuga ugaaauaaua gaacaaauaa gcgaguucuc aaaacgaguc     3840

auuuuagcag augcuaacuu agauaaaguu uuaagugcau auaauaaaca cagagauaaa     3900

ccgauccggg aacaagcaga aaauauaaua cauuuauuca cucuaaccaa ucuaggagca     3960

ccagcagcau uuaaauauuu ugacacaacu auagaucgua aaagauauac uucaacaaaa     4020

gaaguacuug augcgacuuu uauacaucaa ucaauaacag gucuauacga aacgcguauu     4080

gaccuuucac aauuaggugg ggauuga                                         4107


<210>  5437
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5437
augucacggc ccuauaacau ugggcuagac auaggcaccu cuaguauagg auggucugug       60

guagacgacc aauccaaauu aguaucugua agaggaaaau auggguaugg agugcggcua      120

uaugaugaag gacaaacagc agcagaacgu cggaguuucc gaaccacacg aagacgacuc      180

aaaagaagaa aauggagacu cggauuauua cgggaaauau ucgaaccaua cauaacacca      240

aucgacgaca cuuucuuucu aagacaaaaa caaucaaacu uauccccaaa agaccagaga      300

aaacuuuaca cgcaaacuuc ccuauuuaac gaccguacag accgcgccuu cuaugaugau      360

uauccaacaa uauaucaucu uagauauaga uuaaugacag aaaaacgaca auucgauauu      420

cgagaaauau auuuggccau gcaccauauu gucaaauaca gaggacauuu ucuaaaugaa      480

gcuccagucu cuagcuucaa aucaagugag auuaauuuag uggcucauuu ugauagauug      540

aacaccauau ucgcagaccu cuuuuccgaa aguggcuucc agcuagaaac agauaaauua      600

gcugaaguaa aagccuuauu acuugacaau caacaaagug cuucuaauag acaaagacag      660

gcuuuaaguu uaaucuauac ucccucaacg aacaaagcug uagaaaaaca gaacaaagca      720

auagcaacug aacuauuaaa agcuauacuu ggacuaaaag caaaauuuaa uguacuaaca      780

ggcauagaag cagaagaugu aaaagccugg acacuaacau ucaaugcuga aaauuucgac      840

gaagaaaugg uaaaacucga aagcucacua gacgacaaug cacaccaaau uauagaaucc      900

cuucaagaac uauacucugg aguucuucuc gccgggauag ugccagaaaa ccaaagucua      960

agccaggcua ugauaacuaa auaugaugac caucaaaaac accuaaaaau guugaaagcg     1020

guacgagaag cucucgcacc aggcgaccga caaagacuaa aacaggcuua cgaucaauac     1080

guagacggac aagaaaauac aaaggcuuau ucaaaggaag auuuuuaugg agacauaaca     1140

aaagcucuaa agaauaaucc agaucauccc aucguuagug agauaaagaa auuaauagaa     1200

cuagaucaau ucaugccaaa gcaaagaaca aaagauaaug gcgcaauccc acaucaacua     1260

caccaacaag aacucgaucg aauaauugaa aaccagcaac aauacuaccc auggcuagcu     1320

gagcuaaauc cuaacucaaa acggcaaaca guugcaaaau acaaacuaga cgaauuagug     1380

gcauucagag uacccuacua cguaggcccu cuaauuacug cagaacaaca aagacagagc     1440

uccgacgcca aauuugccug gcuaauaaga aaagcagaag gacggauaac acccuggaau     1500

uucgaugaca aaguugacag acaggcuucu gcgaaugaau uuauuaaacg uaugacgaca     1560

acagacacau aucuccucgc agaagacgua uuaccaaaac aaucauugau cuaccaacgc     1620

uucgaaguau uaaacgaauu aaauggucug aaaaucgaug aucaacccau uacuacagaa     1680

cuaaaacaag ccauauuuac agaccuauuc augcaaaaga uaucagucac cgucaaaaau     1740

auccaagauu aucuaguaag ugaaaaacga uaugcuuccc gucccgcaau cacaggacuu     1800

ucagaugaga auaaauucaa cucaagacuc ucaacauacc acgacuuaaa aaugauagua     1860

ggugacgcug ucgaugacgu agauaagcaa gcagauuuag aaaaaugcau cgaauggucc     1920

acaauauucg aagacggaaa aauauacagu gccaaauuga acgaaauaga uuggcucacu     1980

gaccaacaaa gaguccaauu ggcggcaaaa cgcuaucgcg gaugggggag auuaagcgca     2040

aaacuuuuaa cucaaauagu uaaugcaaac ggccaaagaa ucauggaccu auuaugggau     2100

acaacagaca auuucaugag aauaguccau agcgaggacu ucgacaaacu cauaacugaa     2160

gcaaaucaaa ugauguuagc ugaaaacgau guacaagaug uaaucaauga ucuauauacu     2220

agcccacaaa acaagaaagc ccugagacaa auccuauuag uaguuaacga uauucaaaaa     2280

gcaaugaaag gucaagcccc cgaacgcaua cuaaucgagu ucgcucgcga agacgaaguu     2340

aauagccgau uaucagugca acggaaaagg caaguugaac aaguauacca aaacauuagu     2400

aacgaacuuu uaaauaauac agaaaucaga aacgaacuga aagaucuaag uaauucagca     2460

uuaucaaaua caagauuguu ccucuauuuu augcaagggg gucgcgauau guacacaggc     2520

gacucacuga auauugauag acuuucaaca uaugacauag accacauacu cccgcaauca     2580

uuuauaaaag acaacucgcu agacaacagg guccuaguau cucagaaaau gaaccguuca     2640

aaagcggacc aaguacccac agacuucaca ucuguagaac ugggacagaa aaugcaaauc     2700

caaugggaac aaaugcuccg agcuggacuu auaacaaaga agaaauacga uaaucucacg     2760

cuuaauccag aucauauuuc aaaauacgca augaaagguu uuauaaaccg ccaauuggua     2820

gaaacaagac aaguaauuaa acucgcuacg aaccuccuaa uggaacaaua cggagaagac     2880

aauauagaau uaaucacugu caaaucaggc uuaacacauc agaugagaac cgaguucgau     2940

uuccccaaaa auagaaaucu caauaaucac caccaugcgu ucgaugcaua cuuaacagcg     3000

uucguaggau uauacuuacu aaaacgauau ccaaaacuaa aaccauacuu cguauacgga     3060

gaauaucaaa aagcuaguca acaagauaaa uggaggaacu uuaauuuucu aaauggcuua     3120

aagaaagaug aacuuguaga ugaaaacaca gaagcaguca uaugggacaa agaaaguggc     3180

cuagcauauc uaaauaaaau auaucaauuc aagaaaauau ugguaacacg aggaguccau     3240

gaaaauucag gagcacuguu caaucaaacu cuuuacgcag cgaaagauga caaagcaucg     3300

gggcaaggag guaaacaguu aauccccgca aaacaagaca gaaguacggc uuuauaugga     3360

ggauauagug gaaaaacagu cgcguacaug ugcauaguaa ggaucaaaaa uaagaaagga     3420

gaucucuaua aaguaugcgg uguagaaaca agcuggcuag cgcaauuaaa acaauuaaca     3480

gaugaggaua gccaaaaagc auucuuagaa cagaaaauau caccucaauu uacgaaagua     3540

aagaaacaga aaggaaauau aauagaagca gucgaagagu ucgaaguaau agccccacac     3600

auccuaauca aucaacguuu cuuugauaau ggacaagaau uaacauuagg aagcgcaacu     3660

uauaaacaca augaacaaga acuaauauua gacaaaacag cgguaaaacu ccuaaaugga     3720

gcgcuuccac uuacucaauc ggaagaacuc gccgagcaag uuuacgauga aauccuagac     3780

caaguuaugc auuauuuucc auuauacgac acaaaccaau ucagagcaaa acuaagcacu     3840

ggcaaagcug ccuucaugaa auugccgugg aaaucacaau gggacggaaa uaaaauggua     3900

caaguaggcc aacaaguuau auuagauaga auacuaauug gacuacacgc uaacgcagca     3960

augucagacu uaaaagugcu caaaaugaca acuccauucg gaaugauuca acaaucaacc     4020

ggaauuaccu uaucagccga uacccaaaua auauaccaaa guccuacugg auuauucgag     4080

cgaagaguug cccuaagaga cuuauga                                         4107


<210>  5438
<211>  4107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5438
auggauaaga aauacucuau cgggcuagac auaggaacaa acuccguagg augggcaguu       60

auaacugacg auuauaaagu acccagcaag aaauucaaag uacuuggaaa uacagacaga      120

cacucaauua agaaaaaucu aauaggagcu uugcuguucg acuccggcga aaccgccgaa      180

gcaacaagau uaaaaagaac agcaagaaga agauacacca gaaggaaaaa ucgaaucugc      240

uaccuacagg aaauauucag uaacgaaaug gcaaaagucg acgauucauu cuuccauaga      300

uuagaagaau cauucuuagu ugaagaagau aagaaacacg aacggcaucc aauauucgga      360

aauauagucg acgaaguugc auaucaugag aaauacccaa caaucuacca cuuaaggaag      420

aaauuaguag auucuaccga caaagcagac uuacgcuuaa uauaucucgc ucuagcacau      480

augauaaaau uuagagggca uuuucuaauu gaaggagacu uaaacccaga caauagugac      540

guagauaaac ucuuuauaca auuaguacaa acguauaacc aacuuuucga agaaaaccca      600

aucaacgcaa gugguguaga ugccaaagca auauuaucgg cucguuuaag uaaaucccgu      660

cgauuagaaa auuuaauagc acaguuaccu ggugagaaga aaaacggacu uuuuggaaau      720

uuaaucgcac uaagccuagg ccuuacgcca aauuucaaau ccaauuuuga ccuugcagaa      780

gaugcuaaac uucaauuauc aaaagacacg uaugaugacg acuuagauaa ucuuuuagcu      840

caaauaggag accaauacgc ugaucuuuuu cuagcagcga aaaauuuauc agacgcuauu      900

uuauuauccg acauacuacg auuaaacagu gaaauaacca aagccccauu aucagcauca      960

augauaaaaa gauaugacga acaccaccaa gauuuaacac uguuaaaagc acugguuaga     1020

caacaauuac cugaaaaaua caaagaaaua uucuucgacc aauccaaaaa uggauacgca     1080

ggauacauag augguggagc cucccaagaa gaauuuuaca aauucaucaa accaauucuc     1140

gagaaaaugg acggaacaga agaauuacuu gcuaaauuaa acagagaaga uuuauuacga     1200

aaacagcgua cauuugacaa uggaaguaua ccacaccaga uacaccucgg agaacuucau     1260

gcaauccuaa gaagacaaga ggauuuuuau cccuuccuga aagacaauag agagaaaaua     1320

gagaaaauac uuacuuuccg cauaccauac uauguaggac cacuagcccg cggaaauucc     1380

agauucgcgu ggaugacaag aaaaagugaa gaaaccauua caccuuggaa uuuugaagaa     1440

guaguagaca aaggcgcauc agcacaaucg uucauugaaa gaaugacaaa uuucgauaaa     1500

aaucuaccaa augaaaaagu acuuccaaaa cacagcuuac ucuaugaaua cuucacuguc     1560

uauaacgaac uaacgaaagu aaaauacguu acagaaggga ugagaaaacc agccuucuua     1620

ucgggagaac agaagaaagc aaucguagau cuuuuguuca aaaccaauag aaaagucaca     1680

gugaaacaau uaaaagaaga cuacuucaag aaaaucgaau gcuucgauuc aguagagauu     1740

ucagguguug aggacagguu uaacgccagu cuagggacgu aucacgauuu acuuaaaaua     1800

auaaaagaca aagacuuccu agacaaugaa gaaaaugaag acauucuuga agauaucgua     1860

cuaaccuuaa cacuguuuga agauagagaa augauagagg aaagacuaaa aacuuaugca     1920

caccucuucg acgacaaagu aaugaaacaa cuaaaaagaa gacgauauac uggguggggc     1980

agacuaucca gaaaacucau aaauggaauc agggacaaac aauccggaaa aacuauacug     2040

gauuuccuca aaucggaugg auucgcaaau agaaauuuca ugcaauuaau acaugaugau     2100

agucuaacuu uuaaagaaga cauacaaaaa gcacaaguaa guggacaagg cgauucauua     2160

cacgaacaca uagcaaaccu ggcuggcagc ccagcgauca agaaaggcau acuucaaacc     2220

guaaaaguag uagacgaauu gguaaaagua augggacguc auaaaccaga aaauaucgua     2280

auagaaaugg caagagaaaa ucaaacaacc caaaaaggac agaaaaacag ccgcgaaaga     2340

augaaaagaa uagaagaagg uauuaaagaa uuagguagcc aaaucuuaaa agaacacccg     2400

gucgaaaaua cccaguuaca aaacgaaaaa cuguacuuau acuaucugca gaacggccga     2460

gacauguaug uagaccaaga acuagacaua aacagauuau cugacuauga uguagaccau     2520

auagugccac aauccuucau caaagacgau ucaaucgaca auaaaguccu uaccagaucc     2580

gauaaaaaua gagguaaauc ugauaacgug ccaucagaag aaguagucaa gaaaaugaaa     2640

aauuauugga gacaauugcu aaacgcgaag cuaauaacac aaagaaaauu ugacaaucua     2700

acaaaagcag agcguggugg auuaucugaa uuagacaaag cuggauuuau aaaaagacag     2760

uuaguagaaa cccgacaaau aacaaaacac guagcucaaa uauuagacuc caggaugaau     2820

acaaaauacg augaaaacga uaagcuaauu agagaaguca aaguaauaac acuuaaaucc     2880

aaacugguuu cggacuucag aaaagacuuc caauuuuaca agguuagaga aauaaacaau     2940

uaccaccaug cccaugaugc auaccucaac gcugucgugg gaacagcuuu aaucaagaaa     3000

uaccccaagc uagaaucaga guucguauau ggagacuaca aaguauauga cgucagaaag     3060

augauagcaa aguccgaaca agaaauuggu aaagcaacag cuaaauauuu cuucuauagc     3120

aacauaauga auuucuucaa gacagaaaua acacuagcaa auggggaaau aagaaagcga     3180

ccacuuauug aaacaaaugg ggaaacggga gaaauuguau gggauaaagg acgagauuuu     3240

gcaacggugc gcaaagucuu aucaaugccu caaguaaaca uugucaagaa aacugaagua     3300

caaacaggag gguuuagcaa agaaagcauc cucccuaaaa gaaacucuga caaauuaaua     3360

gcaaggaaga aagacuggga uccuaagaaa uauggcggcu uugacucacc aacagucgca     3420

uauagugucc uaguaguagc aaaaguagaa aaaggaaaaa gcaagaaacu uaaaucaguc     3480

aaagaacucc uuggccuaac aauaauggaa agaucaucau uugagaaaaa uccuauagau     3540

uuccuggagg cgaaaggaua uaaagaagua aagaaagacc ucauaauaaa acuaccuaaa     3600

uauagccucu uugaacuaga aaacggcaga aaaagaaugu uagcuucagc cggagaauua     3660

caaaaaggaa augaauuagc acuacccucc aaauacguua acuucuuaua uuuagcauca     3720

cauuacgaaa aacuaaaagg gagucccgaa gauaacgaac aaaaacagcu auuugucgaa     3780

caacauaaac acuaccuaga ugagauaaua gaacaaauau cagaauuuuc uaaaagagua     3840

auauuagccg acgcuaacuu agauaaagua cuaucagcau auaacaaaca cagagacaaa     3900

ccaauaaggg aacaagcaga aaauauuauc caccuuuuca cacuaacaaa ucugggagcc     3960

cccgcagccu uuaaauacuu ugauacaaca auagacagaa aaagauauac caguaccaaa     4020

gaagucuuag acgcuacauu aauccaucaa ucaauuaccg gcuuauacga aacaagaaua     4080

gaccucagcc aacuaggagg cgacuga                                         4107


<210>  5439
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5439
augaaacgua uacuggggcu agauuuaggu accaacucaa uugguugggc ucuaguaaau       60

gaagcagaaa cuguaaacga gaaaagcuca auaguaaaac uugggguuag aguaaauccu      120

cuuacaguug acgaacagaa aaauuuugaa gcagguaaau ccauaccgac aaaugcagga      180

aggacauuaa aacgagguau gaggagaaac cuacaacguu acaaacuuag aagagauuac      240

cuaauacaau guuuaaagga auauggaugg auuaccagug auacucuucu uuaugaaugu      300

ggaaaucgaa cuaccuuuga aacauacaga cuacgagcca aagcgguaga ugaagaaaua      360

gcacuugaag aacuuggcag agucuuacua augauaaaca agaaacgcgg cuacaaaagc      420

aaccgaaaag caaaaucaag cgaugaggga caauuaauag acggaauggc aauagcaaug      480

gauauguauc acaauggcac aacuccaggc gaguauguuu auuccuuacu aucaaaagga      540

aagaaauaca uaccaguauu cuauagauca gaucuccuug acgaguucga ccgaaucugg      600

gacuaucaau gugcauacua cccagacauc cucacaaguc aguucaaaga acugauacgu      660

ggaaaaaguu cacaagucac uagcaaacuu uucaaacaac aauauaaucu auuuacugca      720

gacaacaaag aaaaagauaa aagacuacag gcuuauugua gacgagcuga ugcauuaaag      780

aaaagacugc cagucgaaga gguugcagcc gcacugugcg acguaaacag agaugucaau      840

gcaaguagug gauaucucgg aacaauaagu gacagaucaa agaaauuauu uuucaauaaa      900

caaacagucg ggcaauacuu aaugcuagaa uuagauaaag auucacauuu cagcgugaaa      960

aacgaaguau uuuacaggca agacuaucuc aaugaguuug aagccauaug ggaaaaacaa     1020

gcugaauacc auccugaacu cacaccggau cgaaagaaaa ucuuacggga uguaguaauu     1080

uucuaucaac gacccuuaaa aucccaaaaa gacuugguag cauguuguga acuagaacga     1140

agacggauag aagucaugaa agacgggaaa guaagauccg uagagauagg uccaaaagua     1200

ugccccaaau cuaguccacu auuucaagau uucaaaauuu ggcacguauu aaauaaucua     1260

aaaguacgug auaaagcgac aggagacgaa agauuccuag aauuagauga aaaagaaaua     1320

cuauucagug aacuugccau aaaaucaaaa cucucaaaaa cggaggcuuu gaaauuacua     1380

uaucaaaaac caaaaggacu cgaucucaau uacgauucag uugaaggcaa uaccacgcaa     1440

gcagcacuau uugaagcaua ccaaacagua auugcaauga caggacaugg agaauaugug     1500

uuuagcaaaa uguccgcgag ugaaguaaug agcgucgugg aaggaguauu cggaggauua     1560

gguuucaaca cuucuguauu acgauucgau ucucuuuuac agggaaaaga acuagaaaaa     1620

caagauagcu aucgccucug gcaucuguua uauucauaug aaggagauaa uucgguaacu     1680

ggaaccgacg gccuagucgc aaaaguagca ucucuauuaa gaugcgacga agaauaugca     1740

aaagugguug caggaguuac uuuccaacca gacuauggaa gccuaucagc aaaagcaauu     1800

cgacgaauac ugccacacau gcgagcugga aacgaauacu ccuuagcaug ugaauaugcg     1860

ggguacaaac auagucgaag aucgcuaaca aaagaagaau uagaacaaaa agacuauaaa     1920

gaccaucuag aacuccuucc aagaaauagu cuuagaaauc ccguagugga gaaaauacuu     1980

aaucaaaugg uaaauguugu gaaugcagua guugaugccu acggaaagcc ugaugaaaua     2040

cgaaucgaga uggcuagaga auuaaagaaa agugcaaaag aaagagaaga aaugacagca     2100

gccauaaaca aagcaacuuc agaacaugaa agaauuaaag aaauauuaaa caaagaauuu     2160

ggcauacaac acguaucaca uaacgacaua guucgauaua aacuuuauug ugaacuaaag     2220

aauaacggau auaaaacguu auacucugau acauacauac cgcaagaaaa auuauucucc     2280

aaagaaguag auaucgaaca cauauugccg caagcgaggc uauuugacga cucauucagu     2340

aauaaaacau uagaauaucg aucugucaac auugaaaagu caaaucaaac agcuugggau     2400

uauguagccg cuaaaugggg ugaagaaggu ucacuaagau auaaaaagag gguagacgau     2460

uuacuggcag caggagucau aggaaaauca aaacaugaua aauuacucau gaaacaggaa     2520

gauauuccca ccgauuucau caauagggac uugcgggaua cacaauauau ugcuagaaaa     2580

gcgagagaaa uacuagaaga ccuaguacca augguaguaa gcacaacagg agcaguaaca     2640

gaccgccuaa gagaagauug gcaauuagua gaugugaugc aggaguuaaa uugggacaaa     2700

uaugcaauga aaggcuuaac agaagaaugg acugauaagg acggccgcag aauuaguagg     2760

auaaaagagu ggacgaaaag aaacgaucac cgacaccacg cuauggaugc uuuaaccauc     2820

gccuuuacca aacauucauu cauucaauau cuuaauaauc uaaacgcaag guccgauaag     2880

uccgguucaa ucuaugcuau cgaacaaaaa gaacucuacc gugauaacca cggcaaauua     2940

agguuuauaa ugccauuuga aggauucagg ucagaagcaa aaagacaacu agaaaacaua     3000

cugguaucca uaaaagcgaa gaauaaaguc gucacacgaa auguuaauaa ugcaagaaga     3060

ggaucaggaa ggcaagcaaa aguccaacuc acaccgagag gacaacuaca uaacgaaacc     3120

auauauggca gcauacacag auauguuaca aaauuagaaa aaguuggagg aaacuuugac     3180

ggagaacaaa uagcaaaagu ggccaaccua agguaucgcg aggcccuaca agcucgacau     3240

gaagcauuug gcggcgaugc aaagaaagca uuuacaggaa agaacgcauu agacaaaaac     3300

ccuuuauauc ucaaagacac aagaaccgaa guaccauuaa aaguaaagac cgucucgacu     3360

gaaacccugu auacuaucag aaaagcagug gcaccagauc ucaacguaga uaaaguaaua     3420

gacgcaaaag uaagaaaagu acuagaagaa agauuacaag aauauggagg agacccaaag     3480

aaggcuuuug uaaacuuaga ugaaaauccu aucuacuuga acaaagcaaa agguauaacu     3540

auaaaaagag uagcaauaac uggaguaaau aaugcaauag cacuacauga uaagaaagau     3600

guuggaggac augugauguu agacgcuuca ggucgcaaac aaccaguaga cuuuguuaau     3660

acugggaaca accaucaugu ggcaauauac agagacgcgg aagguagucu acaagaagua     3720

guaguuagcu uuuaugaggc aguagcaaga guaucauuag gucuaccggu aaucgacaga     3780

gaauaccgua aagaagacgg auggcaauuc uuauuuacaa ugaaacaaaa cgaguacuuc     3840

guguuuccaa auccagaaac uggauucaac ccucaagaag uagaccugac ggaugaaaca     3900

aacuacgcuc ucauaucgcc caaucuuuuu agaguacaaa aauucacaau aaaagauuau     3960

uucuuuagac aucaucuuga gacgacaguc gaagauagga aagaauuaaa agauaugaca     4020

uggaaaagau guggcuuagg aggaauuauc ggaauaguaa aagugagggu aaaucauaua     4080

ggcagaaucg uuaguguugg agaauauuga                                      4110


<210>  5440
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5440
auggcuaaaa cauacaauau uggccuugau aucggaacaa auagcauagg gugggccguc       60

guagaugauc aaaaccaccu aaugagcaua aaaggaaaau auggcuaugg aguaagguua      120

uacgacgaag guaaaacggc ugaagaaaga cgcagcuuca ggacaacacg gagaagacua      180

aaacgacgaa aguggagauu gucucuauug cgcgaaauau ucgaaccaca aauuacacca      240

cuggauaaca cuuucuuccu uagacaaaaa caaucaucau uauccucuaa agauaacaga      300

aaauuauauc ccgagauguc auuauuuaau gauaggacag auacagauuu uuauacacag      360

uacccaacaa uuuaccaccu uaguagagac cucaugacua aaaacaaaca auuugacauu      420

agagagaucu accucgcuau guaucacaua gucaaauacc gaggccacuu uuuaaaugaa      480

agucccguau ccucauuuaa aaguucagaa aucaaacuag cggaaaauuu cgagacccuu      540

aaccaaauau ucaacgaaag guucaacgaa agcauguucc aacuagcagu caacgacuuu      600

gacgaucuga aagcacuucu ccugaacgau caacaauccg cuucgaaucg ccagaaaaag      660

gcacuaggua ugauauaugu uccaaaugaa aacaagagcu uagaaaaaca aaauaaagcu      720

auagcaacag aacuccuaaa agcuauauua ggccucaaag caaaauucaa uguucuuacc      780

ggaauagaag ucgacgacgc aaaaccaugg acacuaucau ucaauucuga aucauuugac      840

gaggauauug auaaagcugc aacaaacauc gacgacgaca guagagagau aauugccaua      900

cuucagggac uauacuccgg uauucuacua gcaggcauaa uaccagaaaa ccaaucucua      960

ucagaggcga uggucgaaaa auaugaccaa caugcagcac auuugaaaca auugaaaucu     1020

auauaccccc uguuaagcau cucugacaaa aaggccauac aacaagcaua uaauuauuac     1080

aucgacggcc aagauaacgc aaaagcauac agccgagaag auuucuaugc ugcaacaaca     1140

aaagcucuaa aaagcuacca agacaaugaa acaaucgccg aaauacuaaa acuuauagaa     1200

uuagaacaau ucaugccaaa acaaagaacg aaagauaacg gugccauacc gcaucaauua     1260

caucagcaag aacuagaaag aauaauagag aaucaacaaa aauacuaccc cuggcuugca     1320

gaacucaauc ccaaugaauc aagaagaaaa auagcgaaau auaaauuaga ugaacucgua     1380

gcuuucagag ucccauacua ugucggacca cuaauaaccu cugaacaaca acaacaaucu     1440

agcaauaaaa aguucgccug gaugauccga aaagcagagg gaaaaauuac accuuggaac     1500

uucgacgaaa aaguggaucg acaagcgucc gcuaaugagu ucaucaaacg caugacaacu     1560

acagacacuu aucuauuagc ugaagaugua uuaccaaaac aaucuuuagu auaccagaga     1620

uuugaagugu uaaaugaacu caauggaaua aaaauagacg acguccccau aacaguagaa     1680

caaaaacaac guauauuuaa cgaccuauuu augaugcaag caaguguaag ugugaaaaac     1740

auucaagacu accuaguaag caaaggcuac uuugcaucca gaccagaaau aacaggauua     1800

ucggaugaaa auaaaguaau uaguagauua uccacuuauc augauuuaaa agcuauacuu     1860

gguggaauag uagaugaagc caacaaacag gcagaucuag agaaaugugu ugaaugguca     1920

acaauauucg aagauggcaa aauauucguu gaaaagcuaa acgaaauaga augguuaacu     1980

gacgagcagc gaaaaagauu aucaucaaaa agauaccgag gauggggacg auuaaguaau     2040

aaacuucuaa aucaaauagu aaaugagaac ggacaacgua uaauggauuu auuaugggac     2100

acuacagaca auuucaugag aauaguacau agugaagagu ucgacaaauu aauauccgca     2160

gcaaaccaaa cauuauuaag caaaaacgau gugcaggacg uaauaaauga uuuguacaca     2220

uccccacaaa acaagaaagc auuaagacaa auauuguuag uagugaguga cauccaaaaa     2280

gcaaugaaaa accaagcucc gucgaaaauc cuaauagaau uugcaagaga agaugaagcu     2340

aauccacguc uaucagucca aagaaaaaga caaguugaac aaguauauca aaauaucuca     2400

aaagauauau uaagcgacuc agacguaaaa gccgaacuua aagaccuaac aaacacagcg     2460

cuagcuaaca cuagauuauu ucuuuacuuu augcaaggag gccacgacau guauacuggu     2520

gaaacccuua acauagauag auuauccgaa uaugauauug aucauauauu accucaagcc     2580

uucauaaagg auaacucacu agauaaccgu guguuaguua gugccaaaau gaaccgauca     2640

aaaagugauc agguaccauu aaacgacuau accgaagaac gauucggaag caaaaugcga     2700

guucaguggg aacaaaugcu acgcgcagga cuaaucacca aaagaaaaua ugauaacuua     2760

acauuaaacc cugacagaau aaguaaauac gcuaugaaag gguucauaaa cagacaauua     2820

guagaaacac gacaaguaau caaacucguc acaaauuuac uaauggauca cuaugaagac     2880

gaugaggugc aacuaauaac uguaaaaagu ggacuaauac aucaaaugag agaaauguuc     2940

gacuucccua aaaacaggaa ccuaaauaac caucaccaug cauucgaugc uuaccuaacu     3000

gcauuuguag gaauuuaucu guuaaagaga uacccaaaau uaacucccuu cuuuguuuau     3060

gggucauauc agaaaaacaa uacaaaugac aaauggagaa acuuuaauuu cuugaaugac     3120

cuaaagaaag aagaaguaau agacagcgau ucucaugaag ucauuuggaa uaaagcacaa     3180

ggucuaguag aauuagacaa aauauaucaa uucaagaaaa uauuaguaac aagagaagua     3240

cacgaaaaua ccggagcgcu uuauaaccaa acacuauauu cagccaaaga cgauaaagca     3300

uccggacaag gaggaaaaca acuuauaccg gcuaaacaag acagacccac agcucuauac     3360

ggaggauauu ccgguaagac aguugcauau augugcauag uaagaauuaa aucuaagaaa     3420

gaagacacuu auaaaguaug uggaguaaaa accucauggg ucgccgaacu agaacgauug     3480

ucagacucaa acgagcaauu agcaucauua aaagcuaaac uuaugccuaa auucacaaag     3540

aacaagaagc aaaaaggaaa aacaauucaa guuaccgaag acuucgagau aauaguacca     3600

agaguucuaa uaaaccaacg cuuuuaugau aacggucaag aaauaacacu agguagugcg     3660

accuacaaac acaaugaaca agaguuaaug cucaacaaga aagcaguaaa auuauuaaau     3720

ggccaauuaa guuuaguaga agcagaaaca cucgcuccac aaguauacga ugaaauuuug     3780

aaacaaguaa ugaaauauuu cccucuuuac gauacaaacc aauucagaac aaaacuaaaa     3840

augggaaaag aacaauucau aaaauuacca uggaagaaua aaugggaugg caauaaauua     3900

auucauguug gacaacaaac aguacuagau agaauacuaa uuggauuaca cgccaaugcc     3960

gcaguaucag aucuuaguac guugaaaguu acgacaccau ucggcaaauu acaacaacca     4020

ucagguauug cucuuucccu uaauacuaaa aucauuuacc aaucaccaac gggccuauuu     4080

gaaagaagag uagcgcugcg aggacucuga                                      4110


<210>  5441
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5441
auggauaaac ccuauaacau uggacucgac auaggaagcc auuccauugg auggaccguc       60

gucgaugaua acuccaagcu aguaagaauu aaaggaaaac ucgguuacgg cgucagacuu      120

uuugacgaag guaaaacagc agaagaacga agacaauuua gaaccacccg aagacgucug      180

aaacgccgca aauggcggcu acguuuacua cgagaaauau ucgaaccaca uaucgcaccc      240

uuagacgaaa cauucuuucu ucgccaaaaa caauccaauc uagugccaac ucaaaagaaa      300

uuaccacaac uuucauuauu caaugacaga ucagaugcag aguucuacca caaauauccu      360

accaucuauc aucuaagaaa ugcgcugaug acagaaaaac gacaauucga ccucagagaa      420

aucuacuuag cuauucauca caucguaaaa uaucgaggac acuucuugaa ugcagcaccc      480

gcugcaucau ucaaauccgg aaaauucgau cucgaaaaca aguuugacca acucaaccua      540

uuguucgcaa gcgcauacag cgauuuagac uuuacauuac gaaccgauaa cguagaucag      600

guaaaagcaa uacucuuaaa cgaagaucug agugcuagca cccgacagcg acaagcguua      660

cccuuaauuu aucaaaccuc cgcagaccag aaagcugcua agaaacuaac aaaaucacua      720

gcuacugaau uauuaaaagc uauauuaggc cuuaaagcca aauucgacgu ucuaacaggg      780

aucgaaaccg acgauaccaa agccuggaga cucacauuca augcagaaga uuucgaugac      840

aaguuaaacg aauuaaaugu aaccgacaac gaagccgcga cacaaauaau agaaauuuua      900

cgagagcuau auucuagcau cuuauuggca gcuauagugc ccgaaaauua uacacuauca      960

caagcuauga uggcuaaaua caacgcacau aagcaacauu uaucacuccu aaggcaaguc     1020

aaaacaaccc uaccagcaaa gaaacaaaaa gaacuccaag ccgcguacga cgacuacauu     1080

gaccaacacc uaccauuaga uaaauuuuac gcaaaacuca cgaaagugcu acccaaagaa     1140

aaagacaccg acaauccuca aauaauacaa auccucaacc uaauagaucu cgaucaauuu     1200

uuaccaaaac aacgaaccaa agauaaugga gcgaucccac aucagcucca ccaacaagaa     1260

cucgaucaaa ucauagacaa ccaaaaagca uauuaucccu gguuagcuca auuaaaucca     1320

aacgagaaaa gacagaaaau agcaaaauac aaauuagaug aauuaguagc auuucgagua     1380

ccguacuacg uaggaccucu aauagaagca aaaacucaac agaaaaccuc aggugcaaac     1440

uuugcaugga ugauuaggaa agaaccagga acaaccacuc caauaacacc auggaauuuc     1500

gacgaaaaag uaaaccggca agccucagca aaugaauuua uaaaaagaau gacuacaacc     1560

gacacauacu uguuagguga agauguacua ccgaaacaca gucuuuuaua ccaaagguuu     1620

gaaguacuaa augaacugaa cgguaucaaa auugaucaaa aacgaguuac cguuagacaa     1680

aaacaagcca uauuuaacga ucucuuucua aagcaaccua auguaacagu caagaaauua     1740

caacaauacu uacaagcaaa aggagaauau gcgucagccc cuauaauaac aggauuaucu     1800

gauccaaaga aauuuaacuc ucaauuaagu acauaucacg aauacaagaa aauauucgga     1860

ggccucauag acgacccaga uaaacaagca gacuucgaaa aaugcauaga auggaguacc     1920

aucuucgaag acucacaaau acugaaagac aaauuaaaag agauuaacug guuaacaccc     1980

gaacagcgac acaaaguugc caacaaacgu uaccagggcu gggguagacu uucuaagaaa     2040

cuacucaccc aacuuguuga uuacaacgga caaagaauaa uagacauucu uuggaauaca     2100

acagauaauu uuaugcaaau agugcauuca gaagcauuug acaaagcaau uauaaaagaa     2160

aaccaggcuu uacuaacaca auauaaccca caagcaguca uugacgauuu auacaccucc     2220

ccacaaaaca agaaagccuu gagacaaaua uuacuggucg uaaacgacau ccaaaaagca     2280

cuaaaaggac aagccccaag ccgaaucuua auagaguucg ccagggaagg ccaagaaaau     2340

ccacggcuca guacucaaag aaaacaacaa guagaaaaug cuuauaaaga agcgagcgac     2400

gcacuauuau ccagugccga ggugcgacaa gaauuaaaag gucucgacaa uaaagcacua     2460

gcaaauacac gauuauuccu auacuucaug cagggaggca gggauaucua uacugggaaa     2520

ucauugaaua ucgaugacuu aucaaaauau gacauagacc auauauuacc ccaggcuuuc     2580

auuaaagaua acucucuaga uaacagagua cucguaucgg cuaaaaugaa uagaagcaaa     2640

uccgaccaag ucccauuaaa cgauuuugcc gagauaagau auggaaaaca aaugagacaa     2700

caaugggaga aaaugcuaag agcagggcuu auaaccagga agaaauacca caaucuaaca     2760

uuaaauccgg aaagcauuaa uaaauacaca gccaaaggau ucaucaacag acaacucguc     2820

gaaacaagac aaguaaccaa acuagcaaca aaucuccuag uugaccaaua ugcugaagau     2880

gacguacaac ugauaaccgu aaaaucaaac cuuacacacc aaaugagaga agaauuugau     2940

uuucccaaaa accgaaacgu caacaaccac caccaugccu ucgacgccua ccucacagcc     3000

uucgugggau uauauuuauu aaaaagauau ccuaaacuag cacccuauuu cguauacgga     3060

gauuaccaaa aaggagcauc acaaaauucc aaauggcgua acuuuaauuu ccuugguaac     3120

cuccaccgug agaaaguugu ggacaaaaac acuggagaaa uaauauggca acgagacgca     3180

ggauuaggag aacuaaacag aauauacaau uuuaagaaaa uacugguuac uagagcacuc     3240

agagucaaua gaggcccaau guucaaacaa acaauauaua aagccgaaga agauaaguca     3300

aguggucgug gaucaaaaca acucauaccu acaaagaaaa auagaccuac agaacuauau     3360

ggagguuaua ccaacaaaga aacagcauau uuaugucuug uaaaagaacc ucauaagaaa     3420

ggagauauau auagaauuug cucaguucuu aacauguggc uaacagaauu agacagaauc     3480

gcagacccaa uugcaaaagc acaaaaacua acagagcucu uaacucccca auuuaccaca     3540

acuaaaaaga aacaaggaca gaaaaucccc gucgucaaac cuuuuaaagu cgucauuccc     3600

aaaauacuaa ucaaucaacg auucuucagc agaggccaag aacuaaccgu agcgucacca     3660

auguauaugc auaacgaaca agaacucuuc uuaaacaaga aaaccauaaa auuacuaaac     3720

ggacaauuag cgaaaaacga agcccaugaa uuagcaccua aaauauacca acaaaucuua     3780

gaccaaguaa acgacuacuu uccucuuuac gacauguaua aauucagaga cacuuuaaac     3840

gcaaguaaag aaaucuuugu uaacuuacca uggcaggauc aacgccaaga caagaaaauu     3900

agccaaucag gccaaaaauc cauccuaaau aaaauacuua uaggccucca cgcaaaugca     3960

gcccgaauaa aauuaacaga auuaaacuuc aaaucggauu uugguuuauu aucccaacgu     4020

agcggcauag cuuuuagccc agacgaccaa aucauauauc aaucccccac uggacuauuc     4080

gaacggcgag uuacauuaaa agaccucuga                                      4110


<210>  5442
<211>  4110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5442
augaaaguaa acaauuacca caucggacuu gauaucggaa cauccaguau ugguugggug       60

gcuaugggug aagaugguaa gccacuacgu guaaaaggaa agacugccau aggcgcuaga      120

uuauuucgag aaggaaaccc agcugcggac agaaggaugu uuaggacaac gcgacgcaga      180

cuaaacagga gaaaauggag acucaaauua uuggacgaaa uauucgaccc auacauaacc      240

ccaguagaua guacauuuuu ugcuagauua aaacaaucca acuggucacc aaaagauagc      300

agaaaagcau ucaaagacuc aacacuuuuu cccgaccuaa cagauaaaca auaucacuua      360

gacuacccca caaucuauca ucuucgacac gcuuuaauga cacaggacaa gcaauuugac      420

auaaggaugg uuuaccucgc acuacaucac auaguuaagu acagagggaa cuucuuaaau      480

ucaacaccgg uugacucuuu caaggcauca aaaguaaauu uugaugacca auucaaaaaa      540

cuuaaugaac uauacaccgc aauaaaucca gaagaauccu uccaaauaaa cuuugcaaac      600

ucagaaaaga uaggacaaca auuucuagau ccgucaauac gaaaauuuga caaaaaaaaa      660

caaaucucua aaauaguacc uguuaccaug gaugacaaag cuguagauaa acucaaugga      720

aaaauagcuu cagaaauagu aaacgcaaua cugggauaua aaucaaaacu agauguugcc      780

guccagugcg auccagagga uucaaagcca ugggcuguaa aauuugauga ugaagacaua      840

gaugcgaaau ugcaaaaaau auuaccggaa auggaugaaa accaacaauc aauuaucggc      900

auaauacaag acuuauauag ucaggucaca uuaaaucaaa uaguaccgaa cggaaugagc      960

uuaucagaau caaugauaaa aaaauauaac gaccaccaug accaccuaaa acuuuauaaa     1020

aaauuaaucg gccaaauuuc ugacaguaaa aaaaaaacag cuuuaaaaaa agcauacgau     1080

caauacgucg guaaugacgg gaaaguaaua gaacaggaug auuucuauuc aagcguaaaa     1140

aagaaucuag augacagcga acuaucaaaa cagauuaugg accuaauaga ugccgauaaa     1200

uuuaugccaa aacaacgaac aucacaaaau gggguaauuc cacaucaacu acaccagcgg     1260

gaauuagaug aaauaauaga acaccaaucu aaauacuauc cauggcuagc agaguccaau     1320

ccaaauaaac augaucuuca ccucgccaaa uauaaaauag aagaacuagu agcauuuaga     1380

auaccauacu augucggucc uaugauaacc ccagaagacc aaaaaaaaag uggagcagcu     1440

guguuuucuu ggaugaagag aaaagaaccg ggucaaauua ccccuuggaa uuucgaccaa     1500

aaaguugauc guauggaauc ugcaaauaaa uucauuaaaa gaaugacaac aaaagacaca     1560

uaucuaauag gagaagacgu acuaccagau gaaagccuuc uguacgaaaa auucaaaguc     1620

uuaaaugaac uaaacauggu ucgaguaaac ggugauucac uuaaaguagc agauaaacaa     1680

gccauauauc aagaccuuuu cgaaaauuau aaacacauca gcguuaaaaa aaugcaaaac     1740

uauauuaaag auaaaacagg cuuaccaaau aacccagaua ucuccggcuu auccgacccc     1800

gaucauuuua auaacucacu aggaacuuau aacgacuuca aaaaacuuuu cggcgauaaa     1860

guugaugacc cggaucuaca agaugacuuu gagaaaaucg uugaaugguc uaccguauuu     1920

gaagacaaaa aaauuuuaag agccaaacua aaugaaauaa guugguuaag cgacaaacaa     1980

aaagaugauc uagaaucauc aagauaucaa cgcuggggaa gauugucuaa aaaauuacuu     2040

accggaauag ugaaugacca aggcgaacga auaaucgaug aauuauggaa uacaaacaaa     2100

aauuuuaugc aaauacaauc agaugacgac uucgccaaaa gaauucauga agcuaaugcg     2160

gaccaaauga aagcaacaga uguggaagac guacuagcug acgccuauac aucaccacaa     2220

aauaaaaaag cuauaagaca aguuguaaaa gugguagaug auauacaacg ugccauggga     2280

ggcguagcac caaaauuuau cucaauagag uucacuagau cggaagaucg gaauccaaga     2340

cgaaccaucu caagagaaag gcaacuggaa gauacacuaa aagauacagc aaaaacacua     2400

guaaaaucaa ugaaccccga uauucuaagu gaacuagaua acgcaucaaa aagcaagaaa     2460

ggacuaacag auaggcucua cuuauacuuu acccaacuug gacgagauau guauaccgga     2520

aaaccaauua acauugauga aaucucuaac uaugauauag aucauauauu accacaagca     2580

uuuauaaaag augacucucu uaguaauaga guuuuaguau ccaaagcagu caauaauggu     2640

aagucagcca acgugccuuu aaaacucuuc gguguaaaaa uggcacacuu cuggaaacaa     2700

cuggcagaag ccggucugau caacaagaga aaacuaaaaa aucuccaaac caaccccgau     2760

acaauaucaa aauucgcuau gcauggguuu auaagaagac aacuaguaga aacuucacaa     2820

gugauaaaau uaguagcuaa cauuuuagga gauaaauauc gcaaugacga caccaaaauu     2880

auagaaauua cagcaagaau gaaccaccag augagagaug aguuugcauu uauaaaaaau     2940

agagaaauaa augauuacca ucacgcauuc gaugcauacc ucacugccuu ucuaggucga     3000

uaccuauacc auagauacau aaaacuaaga cccuacuucg uauacggcga uuucaaaaaa     3060

uuucgagaag acaaaguaac aaugcgaaau uuuaacuuuu uacaugauuu aacagaugac     3120

acacaggcaa gaauagcaga caccgaaacu ggagaaguaa ucugggaccg agaaaccuca     3180

auaaaacaac uaagagacgu auaucauuac aaauucaugc uaauauccca ggaaguguac     3240

accuugcgag gagcuauguu caaccaaaca auauacccug cagacgaagc uggcagaaaa     3300

aaguuaauac caacaaaagc agauaaagcg guagauguau acggagggua caguggauca     3360

aacgaugcau acauggcuau aguacgcaua caugauaaaa aaggggacaa auacaaggua     3420

gucggugucc caaugcgugc auuagacagg cuuaaugcag ccaaaaacgu auccgaugca     3480

aaguaugauc aaguuuugaa ggaugugcua acaccgcagu ucacaaaaag uaaaaagagc     3540

agaaagacag gcgaaaucag ccaaacggua gaggacuuug aagugguccu ugguaaaguc     3600

auguaccgac aacuaguaau agacggcgac aaaaaauuca uguuaggauc uagcacauac     3660

caauacaaug ccaaacaauu aguauuauca gaugaaucag ucaaaacauu ggcaucaaaa     3720

gggaaacucg acgaagcuca agagucagca gcauacgaua auguauauga cgaaauauua     3780

gauaaagucg accgauacuu cgcguuguac gaccaaagaa auuacagaac aaacuuacau     3840

gaaggcgaac aaaaauucau aggacuaccg uuacaugaca ccuucgaugg caguaaaaaa     3900

guagcaaacg guaaaagaga aacaaucagc gauauccuga auggacuaca ugcuaacgcc     3960

accaugacaa aucuaaaagu gcucggcauc agcucaccau uuggaauguu ucagucacac     4020

gguccaauaa aucuaaaucc ugaaacgauc auauuauguc aaagcccaac cggacuguuu     4080

gagagacgaa uaauacuucg agaccuauga                                      4110


<210>  5443
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5443
augaaaaaaa gaaauuacaa cauaggccua gacaucggga ccagcucuau aggauggacu       60

aucguggaug auaacgcaca aaucaugaga gcaaaaggua aaaaugguua cggaguaaga      120

auauucgcag aaggaaaaac agccgccgac cgucgaggau uuagaacaac aagaagacga      180

cucaaacgua ggaaauggag gcucagacua uugcaagagu uuuucgaacc uuauauauua      240

cccauagacg aaaauuucuu uaugcgccaa aaagagucaa accuugugcc ccaagaucca      300

aacaaacauu uuaaaggauc cauccuuuuu aaugaccgaa cagaucaaga auuccacgaa      360

caguacccga caauauauca ucucagauac gcacucauga cagaacaccg ucaauucgac      420

guucgagaaa uauacugggc aauucaucac auaguuaaau accgaggcca cuuuuuaaac      480

ucaacacuug caaguaaauu uaaaggaggg gaauuacgau uagcggauaa auuugaaucg      540

cuaaauaaac uuuuccugag aguucaggca gaaacaggcu uugaauuaag cgaagauaac      600

guagacaaaa uaaaaacggu auuacuagac aacggaaaau caaggcuaga ccgacagaga      660

gaaguccuca aaauuguaua uuugacaagu gaagauaaag auacagaaaa aaaacgcaaa      720

gccgucgcga cugaauucau aaaagcaguc cuaggacuaa agaccaaacu auucauaguc      780

cuagcaaaaa auccaacagc agaaacagau uggaaauuca gcuuugacgc agaagacauu      840

gaugacaaac uagauaguau aucggaacaa uuaacagacg acgaccacga aaucguagaa      900

cugauaucug aaauacauuc cauaauuacu cuuacaggug uaguacccga ggguauggga      960

cuuucugagu cuaugauagc uaaauaugac caacaucaau uagauuuaaa aaaacuuaaa     1020

agucuaauga gagaucuaga cgugaaacaa gcaagaaaac uacaagacgc cuaugacgaa     1080

uauauagaug gccuaaaacc aucugaagaa gcauuuuauu cugccguauc aaguguaauu     1140

caaaaaaugg augacccuag agcaaaaaaa auaacagcag aaauagaacu ggaagaguuc     1200

augccgaaac aaagaaccaa agcuaaugga cagauaccac accagcuaca ccaacaagaa     1260

cuugaucaaa ucauagagaa ccagaaagaa uauuacccuu gguuagcaga agaaaauccu     1320

aacaaaagag aacauaaugc agcaccacac aagcucgacg gucuagucac auuuagagug     1380

ccauauuaug ucggccccuu aaucgaagca aguguacaac aaaaaaccag caauagcaau     1440

uuugccugga uggaacguaa aguaaaaaau gacaccuccc gaaucacccc guggaauuuu     1500

gaagagaaag uagaucgaaa ugcgagcgca gaaaacuuca uaaaacgaau gacuaccaaa     1560

gacaccuauc ucuugaacga agauguauua ccucaacaau cucuauuaua ccaacgauac     1620

gagguccuua augagcuaaa uaacguaagg guaaacgaca agaaguuaga aaacaauugc     1680

aaacaagacg uauacaaaga ccuauuuaaa aacaauacaa caguauccau caaaaaauuu     1740

aaagacuacc ucgucgccaa cugcgaauau gccgauaacc caaaaauaga agguuuagca     1800

gacgaaucca uauuccuaag uucccuaucu accgaaaaua aauuuaaaga caucuucggu     1860

gcacagaucg augacccaaa cuacuuugaa gacuuugagc auauuaucga augggcaacc     1920

auauucgaag auaacaagau ucuaagggcc aaacuagacg aaaucaguug gcugaaugaa     1980

agucagaaaa aagaacuugc aaaguuacgg augagaggau gggguagauu aucacgcaaa     2040

cucauugcug aaauaacaga ugaaaauggc agucguauua uagaccuuuu augggaacau     2100

auggacaacu uuaugguagu uguuaaccaa gacguuuuca aagaaaaaau cguagcagaa     2160

aacaaacaag uuuuuaagga auuaaaucca cuagauauaa uaaacgagca auucacauca     2220

ccacagaaca aaaaagcucu uagacaaauu cucuugguug uagcugauau ucaaaaagca     2280

augaauggag uaacaccaag caacauauuc auugaauuug cucgagaaga ugaaagaaac     2340

ccaaggcguu ccauacaacg acuaaggcag guugagaaua uguacagaga uaucucagac     2400

acaguccuag cggauccgga aguccgaaac gaauuaagca acaucaaaaa uacagaacuu     2460

gcuagagaca gagucuucuu guauuuucuc caaggaggca gagacguaua uacuggcgaa     2520

ccuuuaaaca uagauaagcu cuccgaauau gauaucgacc acaucuuacc acaagcguuc     2580

auuaaagaua acucucucga caacagaguu cuuguaagug caucgcuuaa uaggucuaaa     2640

agugauagug uaccguugga ugacuuuaca ucagaaaugu uugguagaaa aauggaacca     2700

cucugggaac aaaugcaaag ggcaggacuu aucuccaaaa aaaagcucgc aaauuuaaaa     2760

uuuaaccccg aaaacauaaa caaauauacu agaaacggau uuauaaacag acaauuagua     2820

gaaacccgac aaguaauaaa acuagccgcg aauauuuuaa aagaucacua uggagaagaa     2880

accgaaaucg uuaacguuaa ggcaggacug acacaucaau uucgcaaaga uuuugacuuu     2940

ccuaaaaaca gaaacguuaa caacuaucau caugcauuug augcuuaccu aacggcccuc     3000

guaggaacuu accugcuuaa aaaauaccca aaauuagaau cauauuuugu auaugguaaa     3060

uuuaaaaaag gagacggcau cacuaaucuc aaaaacuuua auuuccucca cggauuuaac     3120

aaaccaggca aaauuaucaa uaaagaaacg ggcgaaauau uauucgacag agacaaacaa     3180

cucggauaca ugaauucaau auaugaccua agaaaaguaa ucguaaccca cgaauuaagc     3240

acuagacaug gcgcaaugua ugaucaaaca cuauauaaag cgucagauga caaagacagc     3300

gguagaggcg gaaaacaacu cauuagaaaa aaagauaacc uaccaacaga aauuuaugga     3360

ggcuauacag guucaacgga cgcuuuuaug aguauaauac gcaugaaaaa aaaagauaaa     3420

uuauacuaua aaguuauagg cguacccacc cgaguagcac acgaaguguu acaaucagau     3480

gguaaaauua acauauuuac acugaacaaa uuuaucgaac aaaaauuaac aaaagaaaaa     3540

gugaacaaaa aaacaggagc aauuacaaga auaacagauu gguucgaauu ggugauagca     3600

aaaguuaugu acggucaagu uauagacgac ggcggccaac cguuuauguu aggcagcagu     3660

acauuaccgu acaacguaag agaacuauac uuagcgaaag auuuaguaaa aguuauaaau     3720

aaaggaucua caucuaaguc cucauauuua gauguauaug augagcuagu aaaaucauug     3780

aauaaauauu ucccaaugua cgaucgaaac auguuucggc aaaaaauaac agaugcacgc     3840

gacaaauuug aaaaacuacc caaacgcgac aaaguuagca acggcaaauu acaacaaaua     3900

ggacaaguca caauaaucga uaggauacuc auuggauuac acacaaacgc uggaguauca     3960

aacuuaaaag aucuagguau agcgacaccu uucgguuuca ugcaacaagg aaaugguauc     4020

agccuaacga gcacagcuaa aaucauuuau caaucaucca caggauuauu cgaaaaauca     4080

aaagcauuag acgauuuaaa uccgcucgcc uga                                  4113


<210>  5444
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5444
augaauaaac ccuauaguau uggacuagau aucgguacca auagcgucgg uuggagcaua       60

auaaccgaug auuacaaagu accagcuaaa aaaaugagag uuuuaggaaa uacagauaaa      120

gaauacauua aaaaaaaccu aauaggagca cucuuauucg augggggaaa cacugcagca      180

gaccggagau uaaaaagaac cgcaagaagg agauacacaa ggaggcgaaa uaggauucuu      240

uauuugcaag aaaucuucgc cgaagagaug aguaaaguag augauaguuu cuuccacaga      300

cuagaagauu cauuucucgu cgaagaggac aagagagguu caaaauaccc cauauuugca      360

acuaugcaag aagagaaaua cuaucaugaa aaauucccca caaucuauca uuuaaggaaa      420

gaauuagccg auaaaaaaga gaaagcagac uuacgucuaa uauaucucgc auuagcacau      480

aucaucaaau uuagaggaca uuuccuaaua gaagacgaua gguuugacgu acgaaauaca      540

gacauacaaa aacaauauca agcauuucuu gaaauauucg auacaacauu cgaaaauaau      600

gaccuccuaa gucaaaaugu ggacguagaa gcgaucuuaa cugacaagau aaguaaaagc      660

gcaaaaaaag acagaauacu cgcaagauau ccaaaucaaa aauccaccgg caucuuugcc      720

gaauuucuaa aacucauagu cggcaaucaa gccgacuuca aaaaacacuu caaucuugaa      780

gauaagacgc cucuacaauu cgcaaaagau uccuacgacg aagauuuaga gaacuuauua      840

ggacagauag gagaugaauu ugcugacuug uuuucggcag ccaaaaagcu cuacgacuca      900

guauuacuau caggaauacu uacagucaca gaucuaagua caaaagcacc auuaagugcu      960

agcaugauac agagauauga cgaacaccga gaagaccuaa aacagcuaaa acaauuugua     1020

aaagcgaguc uaccagaaaa auaccaagaa auauucgcag acucaucaaa ggauggauau     1080

gccggauaua uagaagguaa aaccaaucaa gaagcauucu acaaguaucu uucaaaacua     1140

cucaccaaac aagaaggauc cgaauacuua cucgaaaaaa uaaaaaauga agauuuucug     1200

cgcaaacaaa gaacguuuga caacggcucc auuccccauc aaguccacuu gacagaauug     1260

agggcaauca uucgaagaca aucggaauac uauccauuuu uaaaagaaaa ccuagacagg     1320

aucgaaaaaa ucuuaacauu ccgaauucca uauuauguug guccccuagc aagagaaaaa     1380

ucugacuucg cuuggaugac cagaaaaaca gaugacucaa uuagaccgug gaauuuugaa     1440

gauuugguag acaaagagaa aucagccgaa gccuuuauac aucgcaugac aaacaaugau     1500

uuguacuuac cagaggaaaa aguccuuccc aaacauucac ugauauacga aaaauucaca     1560

guauauaacg aguuaacaaa aguaagauuc cuagccgaag gcuucaaaga cuuccaauuc     1620

uuaaauagaa aacaaaaaga aacaauauuu aauucacucu ucaaagagaa acgaaaagug     1680

acagaaaaag auauaauaag cuuucuaaau aaaguugacg gauaugaagg caucgcuauc     1740

aaagguauag aaaaacaauu uaaugccuca uuaagcacau accacgauuu aaagaaaauu     1800

cuaggaaaag auuucuuaga caauacugau aaugaacuaa uauuagaaga uauaguacaa     1860

acccugacau uauuugaaga ccgcgaaaug auacgcaaac guuuagauau auauaaagac     1920

uuuuucacug aaucccaauu aaaaaaacua uacagaaggc auuacaccgg uuggggaaga     1980

cugucagcaa aacucauaaa uggaauccga aacaaagaaa aucaaaaaac cauacucgau     2040

uaccugauag augacggauc agcaaacagg aacuucaugc aacuuauaaa agacgccggc     2100

cuaucuuuca aaccuauaau cgacaaggcu agaacaggaa gucacucaga uaacuuaaag     2160

gaaguaguag gagaacuagc aggaucaccc gcaauaaaga aaggcauauu acaguccuug     2220

aaaauaguug acgaacuugu aaaaguuaug ggauaugaac ccgaacaaau ugugguggaa     2280

auggcacggg aaaaccaaac aaccgcuaaa ggacuaagua gacuuagaca acguuuaaca     2340

acccuaagag aaagucuagc gaaucuuaaa ucaaauaucc uagaggaaaa aaaaccuaag     2400

uacguaaaag aucaaguuga aaaccaucac uuaucggacg accgacucuu uuuauacuau     2460

cuacagaacg gcaaagauau guacaccgau gacgagcuug acauugacaa ucugucacaa     2520

uacgacaucg aucucauaau uccacaagca uuuaucaaag acgacucaau ugacaaccga     2580

guccuggugu ccucagcgaa aaacagagga aaaagcgaug acguacccuc aguagaaaua     2640

guaaaagacu guaaaguauu uuggaagaag cuccuagaug cuaaguugau gagucaaaga     2700

aaauacgaca aucucacuaa agcagaacgu ggaggccuua cauccgacga caaagcacga     2760

uuuauacaaa gacaauuggu ggaaacacga cagauaacua aacauguagc uaggauacua     2820

gacgaacgau uuaacaauga acucgauagu aaggguagac gaauacgaaa agucaaaaua     2880

guaacacuaa aaucaaaccu aguaaguaau uucagaaaag aauucggcuu cuacaaaauu     2940

cgugagguaa auaacuacca ccacgcucau gaugccuacc uaaacgccgu uguugcaaaa     3000

gcaauacuaa caaaauaccc ccaacuggaa ccugaauuug uuuacggcga uuacccuaaa     3060

uauaauagcu acaagacaag aaaaagcgcc acagaaaaac uguucuucua uagcaacaua     3120

augaauuucu uuaaaaccaa ggucacccua gcagauggaa ccguuguagu aaaagacgac     3180

aucgagguaa auaacgauac aggugaaaua guaugggaca aaaaaaaaca cuucgcuacu     3240

guaaggaaag uauuaaguua uccccaagua aacauaguaa aaaaaacaga aauacaaaca     3300

ggaggcuuca gcaaagaaag cauauuagca caugguaacu cagauaaauu aauaccacga     3360

aaaaccaaag acauauaccu agacccaaaa aaguauggag gcuuugauuc cccaauagug     3420

gcauauagug uuuuagucgu ugcugauauu aaaaaaggua aagcccaaaa auuaaaaaca     3480

guaacagaac uacuagggau cacaaucaug gaacgaucaa gauucgaaaa aaaccccucu     3540

gccuuccucg aaaguaaagg cuaccuaaau auaagagaug acaaacucau gauacuuccg     3600

aaauauucac uguucgaauu agaaaauggu cggagaagac ucuuagccuc ugcagaugaa     3660

uuacaaaaag gaaaugaauu agcucuccca acacaauuua ugaaauucuu auacuuagcu     3720

agucgcuaca augaauuaaa aggaaaaccu gaagaaauag aacaaaaaca agaauuugua     3780

guacaacaug uaucuuauuu cgaugacauu cuacagauca uaaacgacuu uuccaauagg     3840

gugauauuag ccgacgccaa cuuggagaaa auuaauaaac uauaccaaga caauaaagag     3900

aacauaccug ucgaugaacu cgcaaacaac auuauaaauc uauucacauu uacaagccua     3960

ggagcacccg cagccuucaa auucuucgau aaaauaguag accguaaacg auacacauca     4020

acaaaagaag uacuaaacuc cacucugaua caccaaucca uuacaggacu auaugaaacg     4080

aggauagacu uagguaaacu aggcgaagac uga                                  4113


<210>  5445
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5445
augacuaaga aaaauuacuc aauuggacuc gacaucggua caaacagugu uggcugggca       60

gucaucacag augacuacaa aguuccugca aagaaaauga aaguacuagg aaauacagau      120

aagaaauaua uaaagaaaaa uuuauuagga gccuuacugu uugauucagg agaaacugcu      180

gaagcaacaa gacuaaaaag aacagcaaga agaagauaca ccagaaggaa aaacagacua      240

agauaucuac aagaaauauu cgcagaagaa augacaaaag ucgacgaauc auucuucuac      300

agauuagacg aaaguuucuu aaccaccgau gaaaaagacu ucgaaagaca ccccauuuuu      360

ggaaauaaag cagaggaaga cgcguaccau caaaaauuuc ccacaauaua ccauuuaagg      420

aauuacuuag cggacuccag cgaaaaagca gaucuaagau uaguauaucu cgcacuagca      480

cacaugauca aauacagagg acauuucuua aucgaaggua aacuaaacgc cgaaaauaca      540

gauguacaaa aacuauuuac agauuuugug gguguauacg accgaaccuu ugacgacuca      600

cauuuaagug aaaucacagu agauguugcu ucuacgcuca cagagaaaau aucaaaaagu      660

agacgacuag aaaaccucau caaauauuac ccaacugaaa agaaaaauac uuuauuugga      720

aaucuaaucg cacuggcauu aggauuacag ccaaauuuca aaaugaauuu uaaacucuca      780

gaagacgcua aacuacaauu uucgaaagac acuuacgaag aagaucuaga agaacuucuc      840

gguaaaaucg gagaugauua ugcggaucua uuuacaucag cuaagaaucu uuaugaugca      900

auacuuuuga gugggauucu gacaguagau gacaacagua cuaaagcucc auuaagcgca      960

ucuaugauca aaagauacgu agaacaucac gaagaccuag aaaaauuaaa agaauuuaua     1020

aaagcgaaua aaucugaacu auaccacgau auauuuaaag acaaaaauaa gaauggauac     1080

gcaggauaua uagaaaacgg aguuaagcaa gaugaauuuu acaaauacuu aaagaauaca     1140

uugagcaaaa uagauggauc ggacuauuuc uuagauaaaa uagaacgaga agacuuucua     1200

aggaaacaac ggacuuuuga caauggauca auaccucauc aaauucauuu acaggaaaug     1260

caugcgaucu uaagacgaca aggagauuau uaucccuucc uuaaagaaaa acaagaccga     1320

auagaaaaga uauuaaccuu uaggauaccc uacuauguag gaccauuagu cagaaaagau     1380

agcagauucg cuugggcaga auauagaucc gacgaaaaga uaacucccug gaauuucgac     1440

aaagucauag auaaagagaa aagugcagaa aaauuuauaa caaggaugac acuaaacgac     1500

cuauaccugc ccgaagagaa aguauugcca aaacacucuc acguauauga aacuuacgcc     1560

gucuauaaug aguuaacaaa aauaaaauau gugaaugaac aagggaaaga gaguuucuuc     1620

gacucaaaua ugaaacagga aauauucgac cauguguuca aagaaaaccg aaaaguuacc     1680

aaagaaaaau uacuuaacua ucuaaacaaa gaguucccag aauauagaau caaagaccuu     1740

aucggcuuag auaaagaaaa uaaaucauuc aacgcaaguc ucggaaccua ucacgaccua     1800

aagaaaauau uagauaaggc uuuccuugau gacaaaguua acgaagaagu cauugaagac     1860

auuauaaaaa cauuaaccuu auuugaagau aaagacauga uacacgaaag acuacaaaaa     1920

uauucugaca uauucacggc caaucaacua aagaaauuag aaagacguca cuacaccggc     1980

ugggggagac ucagcuacaa acucauuaac ggaauacgaa acaaagaaaa uaauaaaacc     2040

auauuagacu aucuaaucga cgauggcagc gcaaaccgua auuuuaugca acucaucaau     2100

gaugacaccc uaccauuuaa acaaaucaua caaaaaucuc aaguaguagg agacguagau     2160

gacauugaag ccguaguaca cgaccuucca ggaagcccug caauuaagaa aggaauacua     2220

caaucaguaa aaaucguaga cgagcuaguu aaaguaaugg gggauaaccc cgacaauaua     2280

guaauagaaa uggcuagaga aaaucaaacu acaaacagag gcagaucaca aagucaacaa     2340

agacuaaaga aacuacaaag caguuuaaaa gaauuaggua gcaacauauu gaacgaagaa     2400

aaacccaguu acaucgaaga caaaguagaa aacagucauc ugcaaaacga ccaguuguuu     2460

cuauacuaua uacaaaaugg aaaagauaug uacacgggag augaacuaga cauugaccac     2520

cuguccgacu acgauauaga ucacauaauu ccacaagcau ucauaaaaga ugauaguaua     2580

gauaaccgag uccucacauc auccgcgaaa aacagaggaa aaucagauga cguuccuuca     2640

uuagauauag uaagagcaag aaaggcagaa uggguucgcc uauauaaaag cggauuaaua     2700

ucaaaacgua aauucgacaa ccuuacgaag gcagagagag gagguuuaac ugaagccgac     2760

aaggccggau uuauuaaaag acaauuagua gagacaagac aaauaacgaa gcauguagca     2820

caaauucuag augcacgauu uaacacugaa cacgacgaaa acgauaaagu cauaagggau     2880

guaaaaguca uaacacuuaa aucuaauuua guaagccaau uucgaaaaga cuuugaguuc     2940

uauaaaguaa gagagauaaa cgauuaucac cacgcacacg augcauauuu aaacgcaguu     3000

gucgggacag cguuacugaa aaaguaccca aaacuagcau cagaguucgu uuauggagaa     3060

uacaaaaagu augacauacg uaaguuuaua accaauucuu ccgacaaagc aaccgcaaaa     3120

uacuucuuuu acuccaaccu aaugaacuuc uucaaaacca aagucaaaua cgcagacgga     3180

acaguauucg aaagaccgau uauugaaaca aaugccgaug gagaaaucgc auggaacaaa     3240

caaauagauu ucgaaaaagu ucgcaaagua cucaguuacc cucaagugaa uauagugaaa     3300

aagguagaaa cacaaacagg cggauucuca aaagaaucaa uuuuacccaa gggugauuca     3360

gacaaacuaa uaccaagaaa aacaaagaaa guuuacuggg auacaaagaa auauggagga     3420

uucgauagcc ccacuguugc uuauucagua uuuguaguug cggauguaga aaaagggaaa     3480

gcaaagaaau uaaaaacugu aaaagagcua guagggauau caauaaugga acguucauuc     3540

uuugaagaaa auccgguaga auuuuuagaa aauaaaggcu aucacaauau cagagaagau     3600

aaacuuauaa aauuaccuaa auacucgcua uuugaguucg aagguggacg acgacgacug     3660

uuagccuccg caagugaacu acaaaaaggu aaugaaaugg uacuaccagg cuaucuuguc     3720

gaacuauuau aucacgcaca cagagcagac aauuucaaca gcacagaaua ccuaaacuac     3780

gugucagaac acaagaaaga auuugaaaaa guacuaagcu guguagaaga uuucgcaaac     3840

cuauacgucg acguugagaa aaaccuaagc aaaauucgug caguugcaga uagcauggau     3900

aauuucucaa uugaagaaau uuccaacagc uucauuaacc uccucaccuu aaccgcucua     3960

ggagccccug ccgacuucaa cuucuuaggu gagaaaauac caagaaaacg auauacaucu     4020

acuaaagaau gccugaccgc aacauuaauc caucaaucaa uuacagggcu auacgaaaca     4080

aggaucgacc uaucaaaacu uggagaagaa uga                                  4113


<210>  5446
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5446
augaaaaacg caaauaaagu aacagguaag uacuauuuag gauucgauau agggacaaac       60

ucaguaggau gggcuguuac cgaugagaau uacaaucuau guaaauucaa aaaaaaaagc      120

auguggggaa uaagauuauu ugaagaugca aaaacagcug aagaaaggag aggacaacga      180

gcauccagaa gaagauuaca acgcagaaaa caacguauag auuuacuuca agaaauuuuu      240

gcugaagaaa uaaauaaagu agacgagacg uucuuuauac gacuaaauga aucuagauua      300

cacuuagaug auaaaaccgu aucagaaaaa cacccacuau uuauaggcuc agaauacagc      360

gaaaaggaau acuauagaga guacccaaca auauuccauc uaagaaaaga gcuaauggag      420

aguuccgaac cgcacgauau aagacuugua uacuuagcaa uccaucacau acuaaaaaau      480

agaggucacu uuuugauuga uggagaguug aguaaggcca auaguuucga ugcagcuuuu      540

uaucaaauga aggagguacu augcaaugaa uuagauuuag aaauagacgg cgaaaacaca      600

accgaggucg agcgaacuuu acgagauaga aauauaagua aaucugauaa aguuaaauua      660

uuagccgcuu uauuagcgau gaaucaaaac gaaacagaau caaaagaaac gaaaaaaaaa      720

auagaaaaua uauguaaauu auuagccgga aacaaaggag auauaucaaa aauauuuggg      780

caaacaccag aagggguuga gaaaacaucc uuuucauucg gagaagcaga uuaugaugaa      840

ucgauaagac caaaccuaga agaguccauc cccgaccaau guuucguaau agauagcaua      900

aaagcucugu acgacuggaa cguauuagcu gauauacuaa auggucaaac guacuuaagu      960

ugcgcuaaag uuaaucaaua cgaaacacac aaaaauaauu uaaaagaauu aaagcuacua     1020

guaaaccgcu acugcucaag agaagaauac aaaagguuuu uuaaaagcgc aguagagaaa     1080

gcaaauuaua guaacuauau aggaucugcu aaacagggaa acaaaauaau agauguaaaa     1140

aggugugcag aagaagauuu uuauaaagaa cuuaaaauac uauuaucaaa gauagaaccg     1200

gaagaagcug auagagaauu auaugaaaga auaaaacgag aggccgaaga agguuccuua     1260

cuuccacugc aaaggaauaa agauaauagu gugguaccaa accaaaugca caaagcagaa     1320

cuagcccuaa uacuugaaaa cgcgggaucu uauuuaggau uccugaacga aaaagacgaa     1380

gauggauuaa gcccagcaga aaaaauaauu gaauuauuua gcuucagaau accauacuac     1440

guagguccac uuucagacag acacaaagac cggggaagua acgccuggau agugagaaag     1500

gcagaaggac guauccuccc guggaacuuu gacgaaaaag uagauagaga aaaaaguaau     1560

gaagcuuuca ucgaaagaau gaccaacaaa ugcacauauc uauuaggcaa agauguaguu     1620

gcaaaaaauu cacuaauaua uagucacuuc augguauuga augaacuaaa caauuuaagu     1680

guauguggaa acaaaauauc cgugcaagua aagcaagaca uauaccgcga gcucuuccaa     1740

cgucacacaa aaguaucaaa aaagaugcua uugcaauauu gucgcgaaaa aaugaugaac     1800

aaagauuuag aggaagaaga ucuuucuggg uuugacggag auuucaaagc uaaauuauca     1860

agcuaccuag acuuugaaaa aaaaguauuu ggagcaaaaa uggaggaaga caggauacgg     1920

aaaauaacag aagauauuau aagauggaaa acgauuuacg gugacgaugg aaagaugcua     1980

caagcaguug uagagaagga auacccaggg gaacuggaca gugaccaacu caaagcagua     2040

aaacaauuuc guuacagcgg auggggaaau uuuucagaag aauuuuuaaa cgguaucgua     2100

ggaacugaaa aaccgacagg acagauguuu acaaucauug aagcauuaug gcagacuaau     2160

uguaacuuaa ugcaauuacu aaguaaaaac uucaccuuua uggaggaaau agaaaaacau     2220

aaccgagaac uagcaggcca aauaaccgac auaucuuaug acaaauuagu gaaggaccua     2280

uacauaagcc cagcuaauaa acgagcagua uggcaaacag ugcgaauagc ggaagaaguu     2340

aaaaaaauaa ugggcuguga accugcaaaa auauuuguag agauggcaag aggaggggaa     2400

aaagaaaaaa aacguacgau aucuagaaaa aaaaaauugu uagaauuaua cgcagccugu     2460

aaaaaagaug cacgagacug gaaccgagaa auagaagaca gagacgaaag agaauuuaau     2520

ucagacaaac uauaccuuua uuauacacag auggggagau guauguauuc aggugaaaga     2580

aucgauuugc augaacuaau gagugcgaau ucaaaguggg auauagauca uauauaccca     2640

caaucaaaag uaaaagauga uucucuggac aacagaguuu uaagccuuaa gaaaauuaau     2700

aaugaaaaag gaaauaaacc auuaucuaga gauauucaac agucacagca aaaguggugg     2760

acagagcuau uacaacuaga auucauuacg aaaaaaaaau augaucgacu aaugcaccga     2820

ggugagcuaa gcgaagaaga auuauccgga uuuauaaauc gccaacuggu ugaaacacgu     2880

caaucaacaa aggcugcagc agaauuauua ggaagaaugu auaauaacag caaaguuguc     2940

uacgugaaag cuccacuagu aucacaguuc cgaaaggaug accuaaauau acugaaguca     3000

cgauuagcua acgacuacca ccaugcaaaa gaugcauauc uaaauauagc ugucggcaau     3060

gucuauaaug aaaaauucac agacaaucca cuaagaugga uagaaaaaca cgcuggagaa     3120

aacuacucca uaaacagggu guucgacuau gacauaguag caggcgaugg ugccccggua     3180

ugggcagcac cacuaagaga cgagaaaaau aaacugagga agaaugaggu aggcgaaaaa     3240

uacgguggaa caauagacgu aaucagaaaa accguaaaaa ggaaugaugu acuauacaca     3300

gagcauacau auuguggaaa gggagagcua uuuaaugcaa cgauugcgaa aaaagaugga     3360

ggggccgaaa ucccacugaa agcaggacua caaacuggua aauacggagg guacaaaagu     3420

ccgaacacga gcuauuuugc agcuauugaa uucgacggaa agaaaggcga aagaguuaaa     3480

aauauaaugg aaguaccugu auacauagcg aacauucugu uacacgacau agaugcauau     3540

auaagauacu gcgaaacagu aaaagguuua aaaaacguaa aaauauuacg gccauguauc     3600

aaaaagaacg cgcugauaag cguagauggc uacccaauga ggauacgugg agcgaacgaa     3660

aaaaauauac uacuuaaaaa uaacuuacaa ccaauauuug gacgucacga ggaaacugug     3720

agaaggauag aaaaauaugu agaaaaaaac caagaauuug aagucgacga aaaauuugau     3780

ggauuaaaug aagcaaauuu gacagauuug uacgacgaca uaacagaaaa auuauguggg     3840

cccuaugugg agcguccuag uaaccaaggg gaguaucuca uggaaaauag ggaaaaauuc     3900

cucaauauuu cacuaaaaga aaaagccaag cuucuugcac aaaugauaaa gcuagucaga     3960

ugugauacag aaacaaaagc agaucugaga ucaauaggug cuucuaagaa ugccggagua     4020

aucaguguaa auaagaacac auuaggggga aaaagacuca uauuaauaaa ucaaagcgua     4080

acaggauugu ucgaaaacag aauugaacug uga                                  4113


<210>  5447
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5447
augaacaaac cauacaguau aggauuagau aucggaacca acaguguagg augguccauc       60

auaaccgacg acuacaaagu accagcuaag aaaaugagag uacuaggaaa uaccgauaaa      120

gaauauauaa agaaaaacuu aauuggcgca cucuuauuug acggaggaaa cacagcagca      180

gacagacguc uuaaaagaac agcccguaga agauacacaa gaagacgcaa uaggauucua      240

uaucugcaag agauuuucgc ugaagaaaug agcaaaguag acgacucauu uuuccaccga      300

cuugaagacu cauuccuagu agaagaagac aaacgaggcu cuaaauaucc aaucuuugca      360

accaugcaag aagaaaaaua uuaucacgag aaauuuccaa caauauauca ucuuagaaaa      420

gaacucgcug acaagaaaga aaaagcagau cuacgacuga uuuacuuagc cuuagcucac      480

auaauuaaau ucagaggaca uuuccuaaua gaagaugaca gauucgaugu aagaaacacc      540

gauauacaaa aacaauacca agcauuucuc gaaauauuug acacaacguu cgaaaauaau      600

gaucuauuau cgcaaaaugu agauguugaa gccauucuga cggauaaaau aucuaaaagc      660

gcaaagaaag accgaauacu agcaagauau ccaaaccaaa aaucuacugg aauauucgca      720

gaauuucuaa aauuaauagu ggggaaccaa gcagauuuua agaaacacuu uaaucuugag      780

gacaaaacac cacuacaauu ugcuaaagac uccuacgaug aagaccuuga aaauuuauua      840

ggccaaauag gugacgaauu ugcagaucua uuuucggcag ccaagaaacu auaugacuca      900

guucuccuau cuggaauacu aacaguuaca gacuuaucua cuaaagcacc auuaucugca      960

aguaugauac aaagguauga ugaacacaga gaagaucuga aacaauuaaa acaauuugua     1020

aaggcuuccc ucccggaaaa auaccaagaa aucuucgcag auucuucuaa agauggauac     1080

gcagguuaua ucgaaggcaa aacaaaucaa gaggcuuucu acaaauaucu aucgaaauua     1140

cuaacaaaac aagagggaag ugaauacuua cuagagaaaa uuaaaaacga agacuuuuua     1200

cgaaaacaaa ggacauuuga uaaugguagc auaccacacc aaguacacuu aacggaacua     1260

cgagcaauaa uaagaagaca aucagaauac uauccauucc uaaaagaaaa ucuagauaga     1320

auugagaaaa ucuuaacauu cagaauaccg uacuacguag gaccauuagc aagagaaaaa     1380

ucagauuucg cauggaugac gcgaaaaaca gaugacagca uaagacccug gaauuucgaa     1440

gauuugguag acaaagagaa aagcgccgaa gccuuuauuc acagaaugac aaauaacgac     1500

cuauaccucc cagaagaaaa aguacuacca aaacacagcc uaauauauga aaaauucaca     1560

guauacaaug aacugacaaa aguacgauuc uuagccgaag guuuuaaaga uuuucaauuu     1620

uuaaaccgaa aacaaaaaga gacgauuuuc aauaguuuau ucaaagagaa aaggaaaguc     1680

acagaaaaag auauaaucuc auuucuaaac aaaguagacg gauacgaagg aaucgccauc     1740

aaaggaauug aaaaacaauu caaugccuca uuaucaacau accaugaccu uaagaaaaua     1800

cuaggcaaag auuucuuaga caauacugau aacgaacuca uacuagaaga cauaguacaa     1860

accuuaaccc uuuuugaaga cagggaaaug auacgaaaac gcuuagauau cuacaaagac     1920

uuuuucacug aaagucaacu caagaaacua uaccgucguc auuacacugg cugggggcgu     1980

cuaucagcua aacuaauaaa uggaauaaga aacaaagaaa aucaaaagac cauacuagau     2040

uaucuaauag augacggauc agcuaaccgg aacuucaugc aacuaauaaa agaugcuggc     2100

cuaucauuua aaccaaucau cgauaaagcu agaaccggca gccacucuga uaaccuaaaa     2160

gaaguuguug gggaacuugc aggaagucca gcuauaaaga aaggaauauu acagagcuua     2220

aaaauaguag augaguuagu aaaaguuaug gguuacgagc cagaacaaau aguaguugaa     2280

auggccaggg aaaaucaaac gacagccaaa ggacuaucuc gaucgaggca aagacucacg     2340

acauugcgag aaagucuugc aaacuuaaaa agcaacaucu uagaggaaaa gaaaccgaaa     2400

uacguaaaag accaaguaga aaaucaucac uuaucagaug accgccucuu cuuauacuac     2460

cuacaaaaug guaaagacau guauacagau gacgaauuag acauugacaa ccuaucucaa     2520

uacgacauug accauauaau cccacaagca uucaucaaag augauaguau cgauaaucga     2580

guccugguau caagugccaa aaaucgcggg aaaucagacg acguacccag cgucgaaauu     2640

gucaaagauu guaaggucuu cuggaagaaa cuacucgaug caaaacuuau gagucaaaga     2700

aaauaugaca accucaccaa agcagagaga ggcgggcuca ccucagacga caaagcaagg     2760

uuuauacaaa ggcaacuggu agaaaccaga caaauaacca aacacguugc uagaauauua     2820

gacgaacgau uuaauaauga acuagauagc aaagguagga ggauucguaa aguaaaaauc     2880

guaacacuga aaagcaacuu aguaaguaau uuuagaaaag aauuugguuu uuauaaaaua     2940

agagaaguua auaacuauca ucaugcacac gaugccuauu ugaaugcugu aguggcuaaa     3000

gcaauauuaa ccaaguaucc acaacucgaa ccagaauuug uuuacggcga uuaccccaaa     3060

uauaauagcu acaaaacuag gaaaagcgca acugaaaaac uauucuucua uucaaacaua     3120

augaauuucu uuaaaacaaa aguaacccua gcagacggca cagucguagu aaaggaugac     3180

auugagguaa acaacgauac uggagaaaua gucugggaua aaaagaaaca uuucgccacu     3240

guacgaaaag uacucaguua cccacaagua aacauaguaa agaaaacgga gauacagacc     3300

ggaggauuua guaaagaaag uauauuagcc cauggcaauu cugacaaacu uauccccaga     3360

aaaacaaaag acauauacuu agacccuaag aaauauggug gauucgacag cccaaucgua     3420

gcauacucag ugcuuguagu agccgacauu aagaaaggaa aagcacaaaa acuaaaaaca     3480

gucacagaac uccuaggaau aaccauuaug gaacgaagua gauuugagaa aaauccauca     3540

gcauuuuuag aaagcaaagg auacuuaaac auacgcgacg acaaacugau gauauugccg     3600

aaauacuccc uauuugaacu cgaaaacggc agaagaagac ucuuagcaag ugccggagaa     3660

cuacaaaaag guaacgaacu agcuuuacca acccaauuua ugaaauuucu auaucuagcu     3720

aguagauaca acgaguuaaa aggcaaacca gaagaaauag agcaaaaaca ggaguucguc     3780

guucaacaug uaucauauuu cgacgacauu uuacaaauaa uaaacgauuu cucaaauaga     3840

guaauacuug ccgacgcaaa ccucgagaaa auaaauaaac uauaccaaga uaauaaagaa     3900

aacauaccug uggacgaacu ugccaauaac auaauaaauu uauucacuuu uacgucauua     3960

ggcgcuccag cggcuuucaa auucuucgau aaaauugucg accgaaaaag auacacaagc     4020

acuaaagaag uacuaaacag uacuuugaua caucaaucua uaacaggacu uuacgaaaca     4080

aggauagacc uagguaaguu aggugaagac uga                                  4113


<210>  5448
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5448
augacaaaaa uaaaagacga uuauauuguu gguuuagaua ucgguacuga uucaugugga       60

uggguagcaa ugaacucaaa uaacgauaua uuaaaauuac aaggcaaaac agcuauuggu      120

ucuagacuau uugaaggagg uaaaucggcu gcagaaagac gacuauuuag aacuacccac      180

agaagaauca aaaggagaag auggcgguua aaauuacugg aagaauuuuu cgacccuuac      240

auggcagaag uagauccuua cuucuuugcg cgacuaaaag aaagcgggcu gaguccauua      300

gauaaaagaa aaaccgucuc uucaauagua uucccuacaa gugcagaaga caagaaauuu      360

uacgacgacu accccacuau auaccaucuu cgauacaaau uaaugacaga agacgaaaaa      420

uucgaucuaa gagaagucua uuuagcgaua caccauauaa uaaaauaucg cgguaauuuu      480

cucuacaaua caucagucaa agauuuuaaa gcaaguaaaa ucgacguuaa aucaucuauc      540

gaaaaacuaa augaacuaua ugaaaauuug ggauuagacc uuaacguaga auuuaauaua      600

uccaacaccg cagaaauaga aaaaguacug aaagauaaac aaauauucaa acgagacaaa      660

gucaagaaaa uagcagaacu auuugcuauu aaaacagaca acaaagaaca auccaaaaga      720

auaaaagaca ucucgaaaca gguagcuaau gcuguuuugg gauacaaaac aagauuugac      780

accauagcac uuaaagaaau uucgaaagac gaacuaagug acuggaauuu uaaauuaucg      840

gauauagacg cugauagcaa auuugaagca uuaaugggaa aucuagacga aaaugaacaa      900

gcgaucuuac uaaccauaaa agaacuauuu aacgaaguaa cuuuaaaugg aauaguugaa      960

gacggaaaca cgcuaucaga aucaaugaua aauaaauaua acgaccaccg agaugaucuu     1020

aaacuccuaa aagaaguuau agaaaaccau auugauagga agaaagcgaa agaacuugca     1080

cuggccuaug accuuuaugu uaacaauaga cacgggagac uuuuacaagc caaaaagaaa     1140

cucggaaaaa uaaaaccacg uagcaaagaa gacuucuaca aaguaguaaa caaaaaccua     1200

gaugauagcc gagcgucuaa agaaaucaaa aagaaaauag aauuagauuc cuuuaugcca     1260

aaacaaagaa ccaacgcuaa cggaguaaua ccauaccaau uacaacaacu agaacucgac     1320

aaaauuauag aaaaucaaag caaauacuac ccauuccuga aagaaaucaa cccuguaucu     1380

ucacaucuua aagaagcccc guauaaauua gaugaacuua uaagauucag aguuccuuac     1440

uacguaggac cauuaauuag cccuaacgaa ucaacuaaag acauacaaac uaagaaaaau     1500

caaaacuuug cauggaugau aagaaaagaa gaaggaagaa uaacucccug gaacuucgau     1560

caaaaaguug accgaauaga aagugcuaau aaauucauaa aaagaaugac gacaaaagac     1620

acauacuuau uuggagaaga cguauuaccc gcaaacucau uacuauacca aaaauucaca     1680

guauuaaaug aacuaaacaa cauacgaaua aauggcaaaa gaauaucagu agacuugaaa     1740

caagaaauau acgaaaaucu auucaagaaa cauacaacug uaacagugaa gaaauuagaa     1800

aacuauuuaa aagagaauca caacuuagua aaaguagaaa ucaaaggauu agcagacgag     1860

aagaaauuca auagcggucu uacaacauau aauagauuua aaaaccuaaa uaucuuugau     1920

aaucaaauug acgaccucaa auaucgaaac gacuuugaga aaauaaucga auggaguaca     1980

auuuuugagg auaaaucuau auauaaagaa aaauuaagaa gcauagauug gcuuaaugaa     2040

aaacaaauua acgcguuauc uaauauccga cuacaagggu ggggacgacu aagcaagaaa     2100

cuucuagcac aacuacauga ccacaacggc caaacaauaa uagaacaauu augggacagc     2160

caaaauaacu ucaugcaaau agucacgcaa gcggacuuca aagacgcaau ugccaaagca     2220

aaccaaaauu ugcucguagc cacgucagug gaagacauac uaaauaacgc uuacacauca     2280

ccagccaaca agaaagcaau caggcagguc auaaaaguag ucgacgacau aguaaaagca     2340

gcuucgggca aagucccgaa acaaauagca auagaauuua cuagagacgc ggaugagaac     2400

ccgaaaagau cccaaacaag agguucaaaa cuacaaaaag ucuacaaaga ucucagcaca     2460

gaauuagcau cuaaaacuau agcagaagaa cuaaaugaag caauaaaaga uaagaaacuc     2520

guacaagaua aauauuaccu cuacuucaug caauuaggaa gagacgcuua uacaggagag     2580

ccaauaaaua uagacgaaau acaaaaauau gacauagauc acauacugcc ucaaucuuuc     2640

auaaaagacg augcgcuaga caauagagua uuaguaagcc gagcugucaa uaacgggaaa     2700

ucagauaaug uacccgugaa acuauuugga aaugagaugg cugcaaauuu aggaaugaca     2760

auacgaaaaa ugugggaaga auggaaaaac auuggacuua uuagcaaaac gaaauacaac     2820

aauuugcuaa cagauccaga ucacauaaau aaauacaaaa gugcaggauu uauuagacgc     2880

caacuuguag aaaccucaca gauuauuaaa cugguaucca ccauucuaca aucuagauau     2940

cccaacacug aaauuauuac uguuaaagcc aaauacaauc acuaccuaag agaaaaauuc     3000

gacuuguaua aaucccgcga aguaaacgac uaccaucaug caauagacgc uuaccuuagc     3060

gcaaucugcg gaaaucuauu guaccagaac uacccuaauc uacgaccauu uuucguuuau     3120

ggucaauaca agaaauuuuc cagcgacccc gacaaagaaa aagaaauauu uaacaaaaca     3180

agaaaauuuu cauucaucag ccaaauucug aaaaacaaaa gugagaacuc aaaagaaauu     3240

gccgaaaaac uaaaaagagc guaccaauuc aaauacaugc ucguaucacg agaaaccgaa     3300

acaaaagacc aagaaauguu uaaaaugaca guauuuccga gggaaacgcc ugguaaucua     3360

auacccaaga agaaaggaau ggcaccagaa aucuauggcg guuacacuaa aaauucagau     3420

gccuauaugg uaaucgugaa aauagacaag aagaaagaaa cagaauaccg cauccucgga     3480

auaccuacaa gagagcuagu aaaccucaag aaagcagaaa aagaagacca uuaugaauca     3540

uauuuaaagg aaaucuuagc accuaggauu cuguacaaua aaaauggaaa acgagauaaa     3600

aagauaacau cauucgaaau aguaaaauca aaaaucccau acaagcaagu uauacaagac     3660

ggugacaaga aauuuaugcu gggaucauca acuuacgucu acaaugcuaa acaauuaaca     3720

cuuucaaccg aaucaaugaa agcaauaaca aauaauuuug auaaagauag ugaugaaaac     3780

gacgcacuaa uuaaagcaua cgacgagauc uuagauaagg uagacaaaua ccucccauua     3840

uucgauauua acgcauuccg caaaaagcua cacagcggca gagaaaaauu ugucgaacuu     3900

ucacuggagg acaagaaaga cauaauacua aaaguauuga uaggacuaca cgacaauccu     3960

gcuacauccg acuuaaaaac auuaggauuc ucgagcacug aauuaggcaa aaugcagaaa     4020

aauucaggca uuguacuauc agguaaugcg aaauugauau aucaaagucc aacuggucua     4080

uuuaaaaaga guguuaaaau aagugauuua uga                                  4113


<210>  5449
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5449
augacaaaaa uaaaagacga cuacaucgua ggacuagaca uaggcacaga uucaugcgga       60

uggguugcaa ugaacucaga uaacgacaua cuaaaacuac aaggaaaaac agccauaggg      120

agccaucuau ucgaagccgg gaaaucggcu gcggacagaa gacuauuccg aacaacacac      180

agacgaauaa aaaggagaag guggagacuc aaacuauuag aggaauuuuu cgaccccuac      240

auggcaaaag uagaucccua uuucuucgcu aggcugaaag aaucaggacu uucaccgcug      300

gauaaacgca aaaauguauu uucgauagua uuucccacag cagcagaaga caagaaauuc      360

uaugacgacu auccaacaau auaccacuua agauacaaac uuaugacaga agaugaaaaa      420

uucgaucuac gagaaguaua ccuagccaua caucauauaa uaaaauacag aggcaacuuu      480

cuauauaaca caucuguuaa agacuuuaaa gcuagcaaaa uugauguaaa aucaucaauu      540

gaaaaacuca acgaauugua cgagaaucua ggacuagacu uaaacguaga guucaauauc      600

ucaaacacag cugaaaucga aaaaguauua aaagacaaac aaaucuuuaa aagagauaaa      660

gugaaaaaga uagcggaacu auuugcaaua aaaacagaca acaaagaaca aaguaaaaga      720

auaaaggaca uaagcaaaca gguagccaac gcuguauuag gauauaaaac cagauucgac      780

acuauagccc uaaaggaagu aucaaaggac gaacucucag auuggaacuu uaaacuaucu      840

gacauagacg cagacuccaa auucgaagcc cuaaugggaa auuuagacga aaacgaacaa      900

gcgauauuac uaaccauaaa agaguuauuc aacgcaguaa cucucaaugg cauagucgaa      960

gacgguaaca cucuaucaga aaguaugaua aauaaauaua acgaucacag agaugaccuc     1020

aaacugcuaa aggaaguaau agagaaccac auagacagaa agaaagccgu caaacucgca     1080

cucgcauacg accuauacgu caauaaucgu cauggacaac ucauucaagc caaaaagaaa     1140

cuuggaaaaa uuaaaccugg cuccaaagaa gacuucuaca aaguuguuaa uaaaaaucua     1200

gacgauaguc aagccucaaa agagauaaag aagaaaauag aguuagacag cuucaugcca     1260

aaacaacgga caaaugccaa cggaguuaua ccauaccaac uacaccaacu agaacuagac     1320

aaaauaauag aaaaucaauc aaaauauuac ccauuccuaa aagagauuaa cccagucagc     1380

ucucaucuaa aagaagcacc uuauaaauua gaugaacuaa uacguuuuag agucccuuau     1440

uauguaggcc cguuaaucuc accaaaugaa aguacaaaag auauccaaac caagaaaaac     1500

caaaacuuug cauggaugau aagaaaagaa gaaggccgaa ucacuccaug gaauuucgau     1560

cagaaaguug accgaauaga aucagcaaau aaauucauua aaagaaugac gacaaaagau     1620

accuacuuau ucggagagaa uguucuaccc gcaaacucuc uccuguauca aaaauuuacu     1680

guacuaaacg agcuaaauaa cauaagaaua aauggcaaaa gaauuagcgu ugaccuaaaa     1740

caagagauau augaaaaccu auucaaaaag cauaccacag uaaccguuaa gaaauuggaa     1800

aacuaccuua aacaaaauca uaaucucgua aaagucgaaa uaaaaggccu cgccgacgaa     1860

aagaaauuca acuccggccu cacaaccuau aauagauuca aaaauuuaaa uaucuucgau     1920

aaccaaauag acgauuuaaa auaucgaaaa gauuuugaga aaaucauaga auggaguacu     1980

auauuugaag auaaaucaau uuacaaagaa aagcuacgua gcauugacug guuaaacgag     2040

aaacaaauaa augcacuaag uaacauccgc cuucagggau ggggaagauu auccaagaaa     2100

cucuuagcac aacuacauga ucacaacgga cagacaauaa uagaacaacu augggacuca     2160

caaaauaacu uuaugcaaau agucacccag gcagauuuua aagacgcaau ugcaaaagcg     2220

aaucaaaacc uauuggucgc aacuuccgua gaggauaucc ugaaugaugc auauacgagu     2280

ccugccaaca agaaagcaau acgacaaguu auaaaaguag uagacgauau cguuaaagcg     2340

gcauccggua aaguacccaa acagauagcg auagaguuca cucgugacgc ugacgacaac     2400

ccgaaacgau cacaaacaag agguaguaaa cuccaaaaag ucuauaaaga ucucucaaca     2460

gaacuagcau caaaagcaau ugccgaagaa cucaacgaag cuauuaaaga caagaaacug     2520

guacaagaca aauauuaucu auacuuuaug caacuaggac gugaugcgua uacuggcgaa     2580

ccgaucaaca uagaugaaau ucagaaauau gauauugacc auauccuacc ccaaucauuc     2640

auaaaagacg augccuuaga uaauagaguc cucguaucaa aagcaguaaa caaugacaaa     2700

ucugauaacg uaccaguaaa acucuucgga aaugaaaugg caggaaaucu cggaauaaca     2760

auuagaaaaa ugugggagga auggaaaaau aucggacuaa uaucaaaaac caaauauaau     2820

aaucuacuaa cagacccaga ccacaucaac aaauaugaaa gcgcagguuu uauuagacga     2880

caacugguag aaacuagcca gaucauaaaa cucguaagca caauauuaca aucaagauac     2940

ccuaacaccg aaaucaucac aguaaaagca aaauacaacc auuaccuaag ggaaaaauuc     3000

gaucucuaca agaguagaga agucaaugac uaccaucacg caauagacgc auacuuauca     3060

gcaauaugug gcaaccuccu auaucaaaau uacccuaauc ugagaccauu cuuuguauau     3120

ggccaauaua agaaauucuc aucugacccu gacaaagaaa aagagauuuu uaacaaaacc     3180

cgaaaauuca guuuuauauc gcaaauacuu aaaaauaaau cagaaaacag caaagaaaua     3240

gcagaaaaau uaaaaagagc auaccaauuc aaauauaugc uaguaucuag agaaaccgag     3300

acaaaagauc aagaaauguu caaaaugaca guauuuccca gagaaacgcc cggcaaucua     3360

auuccgaaaa agaaaggaau ggccacagaa auuuauggag gauacaccaa aaauuccgac     3420

gccuacaugg ugauaguaaa aauagacaaa aagaaagaaa cagaguacag aauacuagga     3480

auaccaaccc gcgagcuugu aaaucugaag aaagcagaaa aagaagauca uuaugaaucc     3540

uacuuaaaag aaauauuagc accacguaua uuauacaaua aaaacggaaa aagggauaag     3600

aaaauaacaa guuucgaaau aguuaaaucc aaaauuccgu auaaacaagu cauacaagau     3660

ggagacaaga aauucaugcu uggaucgagu accuacgucu acaacgccaa acaacugaca     3720

uuaucaacag aaucaaugaa agccauaaca aauaauuucg auaaagauuc cgaugagaau     3780

gaugcacuaa uaaaagccua cgaugaaaua cucgacaagg uggauaaaua ucuaccacuu     3840

uucgauauaa acgccuuccg aaagaaacuu cacucaggaa gagaaaaauu cguagaacuc     3900

ucccuagaag acaagaaaga cauaauacua aaaguacuca uaggauuaca ugacaauccc     3960

gcaaccuccg acuuaaaaac auuaggcuuc ucaucgacag aacuugggaa aaugcagaaa     4020

aacucaggaa uaguauugag ugguaaugcc aaacucauau accaaucucc aacaggauua     4080

uucaagaaaa gcguaaaaau uuccgaucua uga                                  4113


<210>  5450
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5450
augaauaaac cauacucaau ugggcuugau auaggcacua acucugucgg augguccauu       60

auaacagaug acuacaaagu ccccgccaag aaaaugagag uauuaggaaa caccgacaaa      120

gaauacauaa agaaaaaccu uauaggagca cuacuauucg acggcggaaa caccgcggca      180

gacagaagau uaaaaagaac ggcaagaaga agauauacac gacgccgcaa cagaauauug      240

uacuuacaag aaauauucgc agaagaaaug ucuaaaguug augauagcuu cuuucacaga      300

cuugaagacu cauuccuagu ugaagaagac aaaagaggca gcaaauaucc uauauucgcg      360

acuaugcaag aagaaaaaua cuaccaugag aaauuuccaa ccaucuacca ucuaaggaaa      420

gaacuagcug acaagaaaga aaaagcagau cuaaggcuaa uauauuuggc ucuagcccac      480

auaauaaaau ucagagguca uuuccuuaua gaagaugacc gauuugaugu ucgaaacacu      540

gacauacaaa aacaauauca agcauuuuua gaaauauucg acacaacauu ugaaaauaau      600

gaccuccuga gucaaaacgu ugauguagaa gcaauacuaa cagacaaaau cucgaaauca      660

gccaagaaag auagaauauu agcgagauau ccuaaucaaa aaucaacagg uauuuucgca      720

gaauuucuca aacuaauugu cggaaaucaa gcugauuuca agaaacauuu uaaccuagaa      780

gauaaaaccc cucugcaauu cgccaaagau ucauaugaug aagaucuuga aaaccuacuu      840

ggucaaauag gcgacgaauu ugcagaccua uuuucugcgg cgaagaaauu auacgacucc      900

guacuacuuu ccggcauauu aacggucacc gaucuaagca cuaaagcccc ucucuccgca      960

ucaaugauuc aacgauacga ugaacauaga gaagauuuaa aacaacuaaa acaauucgua     1020

aaagcaagcc uaccagaaaa auaccaagaa auauuugccg acucaaguaa agauggauau     1080

gcaggauaua uagaaggcaa aaccaaucaa gaagcauuuu auaaauaucu caguaaauua     1140

uuaacaaaac aagaagguuc agaauaucua uuagagaaaa ucaaaaacga agacuuucua     1200

cgaaaacaaa gaacguucga uaauggcuca auaccucauc aaguacaucu gaccgaacuc     1260

cgagcaauca uccgccgaca aucugaauac uaccccuuuu uaaaagaaaa ccucgauaga     1320

aucgagaaaa uucugacauu uagaauaccu uauuacguug gaccacuagc cagagaaaaa     1380

ucagauuucg cauggaugac cagaaaaaca gaugauagca uaagaccgug gaauuuugaa     1440

gaccuagucg auaaagaaaa aucagcagaa gccuucauac aucgaaugac uaacaacgac     1500

cuuuaucuac cagaagaaaa aguauugcca aaacacaguc uuauauacga aaaauuuaca     1560

gucuacaaug aacucacaaa aguacgcuuu uuagcagaag gauucaaaga uuuccaauuu     1620

uuaaacagaa aacaaaaaga aaccauauuc aacucacuau ucaaagagaa aagaaaaguu     1680

acagaaaaag auauaauuuc auucuugaau aaagucgacg guuaugaagg aaucgcaauu     1740

aaaggcauag aaaaacaauu caaugcgucu cucucuacau accaugaccu aaagaaaaua     1800

uuaggaaaag auuuccuaga caauacagau aaugaacuga uuuuagagga cauuguacaa     1860

acacuaacac ucuuugaaga ccgugaaaug aucaggaaac gauuagacau cuacaaagac     1920

uucuuuacag aaucacaauu aaagaaauug uacagaagac auuacacagg cuggggcaga     1980

cucucugcaa aacuaauuaa uggcaucaga aacaaagaga accagaaaac gauacuagac     2040

uaccuaauug acgacggauc cgcaaacagg aacuuuaugc aauuaauaaa agacgcaggc     2100

cuauccuuua aaccaauaau agauaaagcu aggacaggau cacacuccga uaaucucaaa     2160

gaaguaguug gcgaauuagc aggcucacca gcaaucaaga aaggcauacu acaaucacua     2220

aaaauuguag augagcucgu uaaaguuaug ggcuacgaac cggaacaaau cguuguagaa     2280

auggcucgag aaaaccagac cacagcaaaa ggacucucac gaucucgaca gcgacuaacc     2340

acccuaagag aaaguuuggc aaaccuaaaa ucaaauauac ucgaagagaa gaaaccaaaa     2400

uacguaaaag aucaaguaga aaaccaccac cuaucagacg auagauuauu cuuauauuac     2460

cuacaaaacg gcaaagacau guacaccgau gacgaacucg auauagauaa ucuuucccaa     2520

uaugacauug accauaucau uccacaagca uuuauuaaag augauucaau agacaacaga     2580

gugcuagugu caagugccaa aaaucgaggc aaaucagacg acgucccuag cguagaaauc     2640

guaaaagacu gcaaaguauu uuggaagaaa cuacuagacg cuaaauugau gagccaacgu     2700

aaauaugaca aucuuaccaa agcagaaaga ggaggacuca cuuccgauga uaaagcgcga     2760

uuuauccaaa ggcagcuagu ugaaacccga caaauaacaa aacacguggc ucguauacuc     2820

gacgaaagau uuaacaauga acuugauagu aaaggaagaa gaauacgaaa aguaaaaauc     2880

guuacucuaa aaucaaaucu cguaucuaau uuuaggaaag aguucggguu cuacaaaaua     2940

cgugaaguca acaacuacca ccacgcacau gacgcuuacc uaaaugcagu agucgcaaaa     3000

gccaucuuaa caaaauaccc ccaguuagaa ccugaauuug ucuaugguga uuacccuaaa     3060

uauaacuccu acaaaacccg uaaaucugcg acagaaaaac uauucuuuua cagcaacaua     3120

augaacuuuu ucaaaacaaa aguuacacuu gcugacggga ccguagucgu caaggacgau     3180

aucgaaguca auaaugauac aggagaaauc guuugggaca agaagaaaca cuucgcaaca     3240

guuagaaaag uauuaucuua uccacaagug aacauaguua agaaaacuga aauucaaaca     3300

ggaggguuuu ccaaggaauc aauacuagcu cauggcaacu ccgauaaacu aauuccacgu     3360

aaaacaaagg auauuuaccu cgacccuaag aaauauggag gguucgacag ucccaaaguu     3420

gcauacucag ugcucguagu ugcugacauc aagaaaggua aagcccaaaa acucaaaacc     3480

guaaccgagu uacuuggcau aacaaucaug gaaagaaguc gauucgaaaa gaacccaagu     3540

gcauuucuug aaucaaaagg uuaucuuaac auacgugacg acaaauuaau gauacuacca     3600

aaauauucac uauuugaacu cgaaaaugga cgucguagac uccuagcaag ugcaggagaa     3660

cuacaaaaag gaaaugaauu agcacuaccu acacaguuua ugaaauucuu auaucuagcc     3720

ucacgauaua augaacuaaa agguaaaccc gaagaaauag aacaaaaaca agaauuuguc     3780

guucagcaug uaagcuauuu cgacgauauu cuccaaauua uuaacgacuu uuccaauagg     3840

guaauauuag cugacgcuaa cuuagagaaa auuaauaaau uguaccaaga uaauaaggaa     3900

aacauaccgg uggacgaauu ggcuaacaau auaaucaauc ucuucacuuu uacaucacuu     3960

ggugcuccug cagcauuuaa auucuuugau aaaauaguag auagaaaacg auauacuucc     4020

acuaaagaag uauuaaacag cacacugaua cauaaaagca uaacagguuu auaugaaaca     4080

agaauagauc uaggcaaacu aggagaagau uga                                  4113


<210>  5451
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5451
augaauaaac ccuauucaau cggcuuagau auuggaacga acaguguagg cuggaguaua       60

auaaccgacg auuacaaagu acccgccaaa aaaaugagag uacucgggaa uacagauaaa      120

gaauacauca agaaaaacuu aauuggugca uuauuauuug acggagguaa caccgccgca      180

gacagaagau uaaaacgaac agcccgaaga agauacacac gaagaagaaa uagaauauua      240

uauuuacaag aaauauuugc agaagaaaug ucaaaagucg acgacucauu cuuccauagg      300

cucgaagauu cauucuuagu agaagaagac aaacguggau caaaauaccc aauauucgcu      360

acacuucaag aagaaaagga uuaccacgaa aaauucucaa ccauauacca ccuuagaaaa      420

gaacuggccg auaaaaaaga aaaagcugau cuuaggcuaa ucuauauagc cuuagcacac      480

auaauaaaau uuagaggaca uuuucucaua gaggaugacu cauuugaugu aagaaacacg      540

gacauaucaa aacaguauca agacuuucug gaaauuuuca acaccacguu ugaaaacaau      600

gacuuacuua gccaaaacgu ugauguagaa gccauacuga cagacaaaau aagcaagucc      660

gccaaaaaag auagaauacu agcccaauau ccuaaucaaa aaagcacugg uaucuuugcu      720

gaauuccuaa aacuaauagu agguaaccaa gcugacuuua aaaaauauuu uaaccuagaa      780

gacaaaacac cacuucaauu cgccaaagac ucauacgaug aagaccuaga aaaccuucug      840

ggucaaaucg gcgaugaauu cgcagacuua uucagcgcag caaaaaaauu guaugauagc      900

guacuccuua gugguaucuu aacaguaaua gacuuaucca ccaaagcccc cuuaagugcu      960

ucuaugaucc aacguuacga ugagcaccga gaagacuuaa aacaacuaaa acaauucguu     1020

aaagccagcu uacccgaaaa auaccaagaa aucuuugcag auucaaguaa agaugguuac     1080

gccggauaca uagaaggcaa aaccaaucaa gaagccuuuu acaaauacuu aucaaaacua     1140

cuuaccaagc aagaagauag cgaaaauuuc cuagaaaaaa uuaaaaacga agauuuucua     1200

cgcaaacaaa gaacauucga caacggcagc aucccccauc aaguacaccu aaccgaacua     1260

aaagcuauaa ucagaagaca auccgaauau uauccuuuuu uaaaagagaa ucaagauaga     1320

auagaaaaaa uauuaacauu ucguauaccg uacuauauag gaccacuagc aagagaaaaa     1380

agcgacuuug cauggaugac gagaaaaaca gacgacucaa uuaggccgug gaauuuugaa     1440

gaucuagucg acaaagaaaa aucagcggaa gcauuuauuc accgcaugac aaauaaugac     1500

uuuuauuuac cugaagaaaa ggugcuccca aaacacagcc uaauuuauga aaaauucaca     1560

guauauaacg aacuuacaaa aguacgauac aaaaaugaac aaggugaaac uuauuuuuuc     1620

gacucaaaua uuaaacaaga aauauucgac gguguauuca aggaacaccg uaaaguaucc     1680

aaaaaaaaau uacuagauuu cuuggcuaaa gaguaugaag aauucagaau ugucgacguc     1740

auaggccuag acaaggaaaa uaaagcauuc aaugcaagcc uaggaacgua ccacgaccua     1800

gaaaaaauuc uggauaaaga cuuccuagau aauccagaua augaaucaau acuagaagac     1860

auaguccaaa cgcuaacacu cuuugaagau cgagaaauga uuaaaaaaag auuagaaaau     1920

uauaaagacu uauucacaga aagucaacug aaaaaacuau auagaagaca cuauaccggu     1980

uggggacgac uaucugcaaa auuaaucaau ggaauacgag auaaagaguc ucagaaaaca     2040

auacuagauu accuaauaga cgauggccga aguaacagaa auuucaugca auugauuaac     2100

gaugacgguc uaucauuuaa auccaucaua agcaaagccc aagccggguc ucacagcgau     2160

aacuuaaagg aagucguagg agaauuagca ggaucacccg caauuaaaaa aggaauauua     2220

caaucccuca aaauaguaga cgaacuagua aaaguaaugg gauacgaacc cgaacaaauu     2280

guaguagaaa uggcaagaga aaaccagaca acgaaccagg ggcgaagaaa cucacgccaa     2340

cgauacaaau uacuagauga cggcguuaaa aaucuagcau ccgacuuaaa cggaaacaua     2400

uugaaagaau acccaacuga uaaccaagca uugcaaaacg aaagacuuuu uuuauauuac     2460

cuacaaaacg gccgagauau guacaccgga gaagcucuag auauagauaa ccugagucaa     2520

uaugauauag accacaucau cccccaagcc uucaucaaag augauagcau agauaauaga     2580

gucuuaguau caagugcaaa aaaucguggu aaaucagaug acguaccaag ccuggaaaua     2640

guuaaggacu guaaaguuuu uuggaaaaaa uuacuagaug caaaauuaau gagccaaaga     2700

aaauaugaca aucucacaaa agcagaaagg ggaggcuuaa cgucagacga uaaagccaga     2760

uucauucaaa ggcaacuagu cgagacaaga cagauaacaa aacauguagc ucggauucuu     2820

gaugaacgcu uuaacaacga auuagacuca aaaggacguc gaauacgaaa gguaaaaaua     2880

gugacucuaa aaucaaaccu cguaagcaau uucagaaaag aauucgguuu cuauaaaaua     2940

agagaaguga auaauuacca ucaugcucau gacgcauauc uuaacgccgu cgucgcgaaa     3000

gcaauauuaa caaaauaucc ucaacucgaa ccagaauucg ucuauggaga uuauccuaaa     3060

uacaauucau auaaaacucg caaaucagca acugaaaagc uuuucuucua cucuaauauc     3120

augaacuuuu uuaaaacaaa aguaaccuug gcagauggaa ccguaguagu aaaagaugau     3180

auugaaguua auaacgauac uggagaaaua gugugggaua aaaaaaaaca uuuugcaaca     3240

gugagaaaag uacuauccua uccacaaaau aauaucguaa aaaaaacaga aauacagaca     3300

ggaggauucu ccaaagaaag cauucuagca caugguaaca gcgauaaacu cauuccccga     3360

aaaaccaaag acaucuaccu ggacccaaaa aaauacggug gauuugacuc cccaauagua     3420

gccuauagcg uucuuguagu ugcagacauu aaaaaaggaa aggcacaaaa auuaaagaca     3480

guuaccgaac uacuuggcau uaccauuaug gagagaagua gauucgaaaa gaauccaagc     3540

gcauucuuag aaagcaaagg auaucugaac aucagagcag auaaauuaau aauucuacca     3600

aaauauucuc ucuuugaauu agaaaaugga cguagaagac ugcuggccuc cgcaggggaa     3660

cuccaaaagg ggaacgaacu ggccuuaccc acucaauuua ugaaauuccu uuaucuagcg     3720

uccagguaua acgagagcaa aggaaaaccc gaggaaauag aaaaaaaaca agaauucguc     3780

aaucaacacg ucagcuauuu cgaugacaua cugcaauuaa ucaaugauuu cagcaaacgu     3840

gugauacuag ccgacgcuaa cuuagaaaaa auuaacaaac uauaucaaga uaacaaagaa     3900

aauauaucag ucgaugaauu agcaaauaac auuauuaacc uauucaccuu caccaguuua     3960

ggagcgccag cagcauuuaa auuuuuugac aaaaucguag accgaaaaag auauacauca     4020

acgaaagagg ugcuuaauuc aacauugaua caucaaagca uuacagggcu auacgaaaca     4080

cgcauagauc uugguaaacu uggagaagac uga                                  4113


<210>  5452
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5452
augaauaaac cauacucaau ugguuuagau auaggaacaa auucaguagg auggucaauu       60

auaacagaug auuauaaagu gccagcaaag aaaaugagag ugcuagguaa cacugauaaa      120

gaauacauua agaaaaaucu aauuggagcc cuccuguuug acggagguaa cacagccgcu      180

gacaggagac uaaaaagaac ugcacggcga cgauauacaa ggcgaagaaa uagaauauua      240

uaccuacaag aaauauucgc agaagaaaug ucaaaagucg augacucauu cuuucaucgu      300

cuugaagauu cauuucuagu agaagaagac aaacgaggcu cuaaauaucc gauauuugca      360

acuuuacaag aagagaaaga cuaccacgaa aaauuuucaa caauuuauca ccuaagaaaa      420

gaacuagcug auaagaaaga aaaagccgac uuaagacuaa uauacauugc ccuagcccac      480

auaaucaaau uccgaggaca cuuucucaua gaagacgacu cauuugaugu uagaaauaca      540

gacauaucca aacaauauca agacuuucua gaaauauuca acacuaccuu cgaaaauaau      600

gaccuccuau cacaaaacgu ugacguagaa gcaauauuga cagacaaaau cucgaaaagc      660

gccaagaaag auagaauacu ugcacaauau ccaaaccaga aaucaacugg aauauucgca      720

gaauuucuaa aacuaauugu cggaaaccaa gcagauuuua agaaauauuu uaacuuagaa      780

gacaaaacac cccuacaauu ugcuaaagac ucauaugacg aagacuuaga aaaccucuua      840

ggucaaauag gagacgaauu ugcagaucua uucucagcag ccaagaaacu uuaugacuca      900

guacugcuau cgggaauacu cacgguaaua gaucuaucaa ccaaagcgcc ucuaucagca      960

uccaugauac agagauacga cgaacaccga gaagacuuaa aacaacucaa acaauuuguc     1020

aaagcaucau uaccagaaaa auaucaagaa auauucgccg auaguucuaa agauggauau     1080

gcaggauaua uagaaggaaa aacaaaccaa gaagcguucu auaaauaccu aagcaaauua     1140

cuaacgaaac aggaagacag cgaaaacuuu cuagagaaaa ucaaaaacga agauuucuua     1200

agaaaacaaa ggaccuucga uaacgguagu aucccccacc aaguacacuu aacggaauug     1260

aaagcaauaa ucagaagaca gucugaguac uaccccuucc uuaaagaaaa ccaagaucga     1320

auagagaaaa uauugaccuu uaggauucca uacuacauag ggccauuagc ccgagaaaaa     1380

uccgauuuug cauggaugac ccgcaaaaca gacgauucaa uaaggccaug gaacuucgaa     1440

gacuuagucg acaaagaaaa auccgcggag gcuuucauuc accgaaugac caacaacgac     1500

uucuaucuac cagaagaaaa aguacuaccg aaacauuccc ugauauacga aaaauucaca     1560

gucuacaacg aacuaacaaa aguaagguau aaaaacgaac aaggugaaac auauuucuuc     1620

gacucaaaca uaaaacaaga aaucuuugac ggagucuuca aagaacaccg aaaaguaagu     1680

aaaaagaaau uacuagacuu ccuugcaaaa gaauacgaag aauuucggau aguggaugua     1740

auaggcuuag acaaagaaaa uaaagcauuc aacgcaucac uaggaacaua ccacgaccug     1800

gagaaaauuc uagacaaaga cuuccucgau aauccagaua acgaaucaau acuugaagac     1860

aucguacaaa cacucaccuu guucgaagau agagaaauga uuaagaaaag acuugaaaau     1920

uacaaagacu uauuuacuga aagccaauua aagaaacuau acagacgaca uuacacagga     1980

uggggacgac uaagcgcaaa acuaauaaau ggcauccgug auaaagaguc ccagaaaaca     2040

auacuagauu accuaauaga cgauggacgc aguaaucgga auuuuaugca acuaauaaac     2100

gaugaugguc ucaguuucaa aucaaucauu ucgaaagcac aggcaggaag ucauagugau     2160

aaccugaaag aaguaguugg agaauuagcc ggaaguccag caaucaagaa aggcauacuu     2220

caaucgcuaa aaauaguaga ugagcuagua aaagugaugg gauacgaacc cgaacaaaua     2280

guaguagaaa uggcacgaga aaaccaaacc accaaucaag ggagacgaaa cucucgacaa     2340

cgauacaaau uauuagauga cggagugaaa aaccuagcga gugaucuaaa uggcaauaua     2400

cuaaaagaau acccuacuga caaucaagca cuacaaaacg aaagacucuu cuuauacuac     2460

cuucagaaug gacgagauau guauaccgga gaagcgcuag acauagacaa cuuaucacag     2520

uacgacauug aucacaucau accccaagca uuuauaaaag augacucuau agacaaucga     2580

guguuaguau ccagugcuaa aaacagagga aaauccgaug auguuccuag ucuagaaaua     2640

gucaaagacu guaaaguuuu cuggaaaaag uuacuagacg caaaacuuau gucacaacgc     2700

aaauaugaua acuuaacuaa agccgaaaga ggugggcuca caucugauga caaagcucga     2760

uuuauacaaa ggcagcuagu agaaacgagg caaauaacaa aacacguagc acgaaucuua     2820

gacgaaaggu ucaacaacga acucgacagu aaaggucgac guauaagaaa agucaaaaua     2880

guaacccuaa aauccaaucu agucuccaac uuuagaaaag aguuuggcuu cuauaaaaua     2940

agggaaguaa acaauuauca ucaugcacau gacgcuuacc uaaacgcagu aguagcaaaa     3000

gcaauacuaa caaaauaucc ccaauuagag cccgaguucg uauacggaga uuauccaaag     3060

uacaacucuu acaaaacaag aaagagcgcu acugaaaaac uauucuuuua uagcaauauc     3120

augaacuucu uuaaaaccaa agucacccua gcagacggca ccgucguagu aaaagaugac     3180

auagagguua auaaugauac gggagaaauu guaugggaca agaagaaaca cuucgcuacc     3240

guuagaaaag uguuaagcua cccgcagaac aacauaguca agaaaacaga aauacaaaca     3300

ggaggauucu ccaaagaauc aauucuagcc cauggcaacu cggacaaacu uauuccaaga     3360

aaaaccaaag auauauaucu agaccccaag aaauauggcg gauuugauuc accaauugua     3420

gcauacucag uucuaguggu cgcggauauu aaaaagggua aagcccaaaa guuaaaaaca     3480

gucacagaau uauuaggaau cacuaucaug gaaagaagca gguuugagaa aaaccccagu     3540

gcauuuuuag aauccaaagg auauuuaaau auaagagacg acaaacuaau gauacuacca     3600

aaauauucac uauuugaacu agaaaaugga cgcagaagac uacuagcuag cgccggagaa     3660

cuacagaaag guaaugaauu agcauuaccc acccaauuua ugaaauuccu uuacuuagca     3720

aguagauaca acgaaagcaa aggcaaaccc gaagaaauag agaagaaaca agaauuugua     3780

aaccaacaug uaaguuacuu ugaugauaua cuucaacuua uaaacgauuu uaguaaacgg     3840

guuauauuag cagaugcaaa ucuagagaaa auaaauaaac uauaucaaga uaauaaagaa     3900

aacaucucag uggacgaguu agcgaacaau auaauaaauu uauuuacauu cacaucacua     3960

ggagcuccag cagcauucaa guucuuugau aaaauagucg auagaaaaag auacacuagc     4020

acaaaagaag uacuuaauuc uacucuaaua caucaaucaa ucaccggauu auaugagacc     4080

cgcauagauc ucggaaaacu cggggaagac uga                                  4113


<210>  5453
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5453
augaacaaac cuuacucaau cggacuagau auagggacaa acucaguggg augguccaua       60

auuaccgacg acuauaaagu uccagcgaaa aaaaugcgag uacucgguaa uacagauaaa      120

gaauacauca aaaaaaaucu aaucggagcu uuauuauucg auggaggaaa caccgcggcc      180

gauagacguc ugaaaagaac agccaggcgg cgauacacga gacgccguaa uagaauauua      240

uaccuacaag agauauuugc agaagaaaug uccaaaguug augacuccuu uuuccauaga      300

cucgaagauu cauucuuagu ugaagaagau aaacgaggcu caaaauaucc cauauuugcu      360

acgaugcaag aagagaaaua uuaucaugaa aaauucccaa ccauauacca ccuaaggaaa      420

gaauuggcug acaaaaaaga gaaagcugac cugcgucuca uauaccuagc acuugcacac      480

auaaucaaau uccgagguca cuucuuaauc gaggacgaua gauuugaugu cagaaacacu      540

gauauucaga aacaauacca agcguucuua gaaauauucg acacaacauu cgaaaacaau      600

gauuuacuua gucaaaacgu ugacguagaa gcaauauuaa cagacaaaau cucgaaaucu      660

gcaaaaaaag aucgaauuuu agcaagauac cccaaccaaa aaaguaccgg aauauuugca      720

gaauuuuuaa aacuaauagu aggaaaccag gcagacuuua agaaacauuu caaccuugaa      780

gacaaaacac cccuacaauu cgcaaaagac ucauaugaug aagauuuaga aaaccuuuua      840

ggacagauag gagacgaauu ugccgauuua uuuagugcug caaaaaagcu cuacgauagu      900

gucuuacuca gcggcauccu aacagucacc gaccuaucca caaaagcacc gcuaucugca      960

ucuaugauac aaagauauga ugaacaucgg gaagauuuga aacaacuaaa acaauucguc     1020

aaagcgucuc uuccagaaaa guaccaagaa auuuuugcgg auucuagcaa ggacggauau     1080

gcugguuaca ucgaaggaaa aacaaaccaa gaagccuucu acaaauacuu aucaaaacuu     1140

uugacaaaac aagaggguuc agaauacuua uuagaaaaaa uaaaaaauga agauuuccua     1200

cgaaaacaga gaacauuuga caaugguuca auuccccacc aaguccaccu aacagaauua     1260

agggcaauca uccgacgaca aucugaauau uacccacuac uaaaagagaa ccuagaucga     1320

auagaaaaaa uacuaacauu ucgaauccca uacuacgucg ggccacuagc aagagaaaaa     1380

ucagacuucg cauggaugac acguaaaacc gaugacucca uacgaccaug gaauuucgaa     1440

gauuuaguug acaaagagaa aagugcagaa gcauucauac accgaaugac aaacaacgau     1500

cucuaucuuc ccgaagaaaa aguauuacca aaacacucac uaauauacga aaaguuuacc     1560

guauacaacg aacuaacaaa agucagguuu uuagccgaag gcuuuaaaga uuuucaguuu     1620

cuaaauagga aacaaaaaga aaccauauuc aauucauuau uuaaagaaaa acgaaaagua     1680

accgaaaaag auauaauaag cuuucuuaau aaaguugacg guuaugaggg aauagccauu     1740

aaaggcaucg aaaaacaguu uaacgcaagc cuuagcacuu accacgaccu aaaaaaaauc     1800

uuaggcaaag acuuucuaga uaacacagau aaugaauuaa uacucgaaga uauaguccag     1860

acauuaacgc uauucgagga ccgagaaaug auucguaaac gacuggacau uuacaaagau     1920

uucuuuacug aaucacaacu caaaaaacuu uaccgccgac auuauacagg cugggguaga     1980

cuaucugcaa agcugauaaa uggcauuaga aacaaggaaa accagaaaac aauucuagac     2040

uacuuaauag acgacggcuc cgcaaauaga aacuuuaugc aacuaauuaa agaugcggga     2100

cuaucauuca aacccauuau cgacaaagcu cgcacgggau cacacucuga caauuuaaag     2160

gaaguaguug gagaauuagc agguucccca gcaauuaaga aaggcauccu acaaucauua     2220

aaaauaguug augaacuagu aaaagucaug ggauacgaac cugaacaaau aguaguagag     2280

auggcaagag aaaaucaaac uacggcaaaa ggauuaucaa gaucaagaca aagauuaacg     2340

acacuuagag aaagcuuagc caaccugaaa ucaaauaucc uagaagaaaa aaaacccaaa     2400

uauguuaaag accaaguaga aaaucaccac cuaucagacg aucgucucuu ucuauacuau     2460

cuacaaaaug gcaaagacau guacacagau gaugagcuag acauagauaa ucucucccaa     2520

uaugauauag accauauuau cccacaagca uucaucaaag acgauuccau cgauaaccgc     2580

guacucguau ccucagccaa aaauagaggu aaaucagaug acguccccuc uguagaaaua     2640

gugaaagauu guaaaguauu uuggaaaaag uuacuugaug cgaaacuaau gagccaacgc     2700

aaauacgaua accuuacaaa ggcugaaaga ggaggucuca cauccgacga caaagcaaga     2760

uucauccaac gacaacuagu agaaacaagg caaauuacua aacacguggc uagaauacuu     2820

gacgaaagau uuaauaauga auuagacucu aaaggaagaa gaauacguaa agugaaaauc     2880

guaacacuaa aaagcaauuu aguaagcaac uuucgcaagg aauucggauu cuacaaaaua     2940

cgcgaaguaa auaauuauca ucaugcucau gacgccuacc uuaaugcagu uguagcaaaa     3000

gcaauucuaa caaaauaucc acaauuagag ccagaauucg uauauggaga uuacccaaaa     3060

uacaacucau auaaaacuag aaagagcgca acagagaaau uauuuuuuua cagcaauaua     3120

augaacuucu ucaagacaaa aguaacauua gcagauggaa caguuguugu aaaagaugac     3180

auugagguaa acaacgauac aggcgaaauu gucugggaca aaaagaagca cuucgcgaca     3240

guaagaaaag ucuuaucaua cccacaagug aauauaguua aaaagacgga aauccaaaca     3300

gguggcuuua guaaagaguc cauauuagca cauggcaaua gugacaaacu aaucccgaga     3360

aaaacaaaag acauuuacuu agaucccaaa aaauauggag guuuugacag cccaauagua     3420

gcauauucug uucugguugu ugcagacaua aaaaagggca aagcacaaaa auuaaaaaca     3480

guaacagaac uacuaggcau aaccauaaug gaaagaucga gauuugaaaa aaacccuagc     3540

gcauuccucg aaucaaaagg auaccuugau auacgagacg acaaacuaau gauauuacca     3600

aaauacagcc uuuucgaacu cgaaaacggu agaagaaggu uauuagcuuc cgcuggggaa     3660

cuacaaaaag gaaaugaacu agcucuacca acacaauuua ugaaauucuu auaccucgca     3720

agcagauaca augaacuaaa aggaaaaccu gaagaaauag aacaaaaaca agaguucguc     3780

gugcaacacg ugucuuauuu cgaugacaua cuucaaauca uuaacgacuu uucaaacagg     3840

guaauccugg cugaugccaa ucuugaaaaa aucaauaaau uguaucaaga uaacaaagaa     3900

aacaucccug uggaugaacu cgcaaacaau auaauaaacu uauuuacauu cacaucauua     3960

ggugcaccag cugcauucaa auuuuuugac aaaauagugg acagaaaacg uuacacaagu     4020

acaaaagagg uacuuaacag uacucucaua caccaaagca uaaccgggcu auaugaaacu     4080

cgaauagacu uagguaaauu aggagaagau uga                                  4113


<210>  5454
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5454
augaagaaac cauauuccau cggacucgac auaggaacua acucagucgg uugggcaguu       60

guaaccgacg acuauaaagu cccugcuaaa aagaugagag uauuugguga uacagaucga      120

ucucacauua agaaaaaccu ccuaggaacu cuacuauuug augacggaaa cacggcagaa      180

ucaagacgau uaaaacgaac ugccagaaga agauacacua gaagaagaaa cagaauuuua      240

uaccuacaag aaauauuuac cgaauccaug aacgaaauag augagagcuu cuuccaccga      300

cuggacgauu cauuuuuagu accagaagau aaacguggaa gcaaauaucc cauauuugcu      360

acauuacaag aagaaaaaga auaucauaaa caauuuccca ccauauacca ccuaagaaaa      420

caacuugcag acuccaaaga aaaaucugac guccgacuca ucuaccucgc uuuagcacac      480

augaucaaau acagagguca uuucuuguau gaagaaacuu ucgacaucaa aaacaacgac      540

auacaaaaga uauuuaacga guucauaaau aucuacgaca auacauucga agguagcagu      600

cuuaguggac aaaacgcuca aguagaagcg auauuuacag auaaaauaag caaaagugcg      660

aagcgcgaac gaguccuaaa acuuuuccca gaugaaaaau ccaccggccu guuuucagaa      720

uuucuaaagu uaaucguugg aaaucaagcc gacuucaaga aacacuuuga ccuagaagaa      780

aaagccccac uucaauucuc aaaagacaca uaugacgaag aauuggagaa ucugcucggu      840

caaauaggag acgacuuugc agaccucuuc uuaauagcua agaaauuaua ugacgcuaua      900

cuacucucgg gcauuuuaac gguuacugac ccuaguacaa aagcaccccu uuccgccagc      960

augauugaaa gauacgaaaa ccaccaaaaa gaccucgccg cccuaaaaca auucauuaaa     1020

aauaaccuac ccgaaaaaua uguggaagua uucagugauc aguccaaaga cggauaugca     1080

ggauauaucg augggaaaac cacucaggaa gcauucuaua aauauauuaa aaaccugcua     1140

ucaaaauuug agggagcaga cuauuuucuu gauaaaauug agcgagaaga uuuccucaga     1200

aaacaacgaa cuuuugauaa cggcucaauc ccucaccaaa uucacuuaca agaaaugaau     1260

gcuaucuuac gaagacaagg ggaacacuau ccuuucuuaa aagaaaaccg agaaaagaua     1320

gagaaaaucc uaacguucag aauacccuau uacguaggac cacucgcaag ggauaauaga     1380

gauuuuucau gguuaacgag aaacucugac gaacccauua gacccuggaa uuucgaagaa     1440

guagucgaua aagcuagauc agcagaagac uuuauacauc gaaugacaaa cuacgaccuu     1500

uauuugccag aagaaaaagu ccuaccaaaa cauucauuac uauacgaaac uuucgcaguc     1560

uacaaugaac uuacaaaagu caaauucauc gcugaagguu uaagagauua ccaauuucua     1620

gauucuggac agaagaaaca aaucguuaac caacucuuua aagagaaaag aaaaguuaca     1680

gaaaaagaca uaauacacua ucuacacaac gucgacgguu acgacggaau agaauuaaaa     1740

ggaauagaaa aacaauucaa cgcaaacuua uccaccuauc acgaccucuu aaaaaucaca     1800

aaagacaaag aguucaugga ugaccccaaa aaugaagaaa ucuuagaaaa cauuguccau     1860

acccuaacca ucuucgaaga ccgagaaaug aucaaacaaa gacucgcuca auacgacaca     1920

uuguucgacg aaaaaguaau uaaagcacua acacgucgac acuacaccgg auggggaaaa     1980

uuaucagcaa aacuaauaaa cgggauaaga gacaaacaau cugguaaaac gauacuagac     2040

uaccuaauug acgacgauaa aauaaacaga aauuucaugc aacuaauaaa ugaugacggu     2100

uuaaguuuua aagaaaucau ucaaaaagca caaguagucg gaaaaacaga cgacguaaaa     2160

caagugguac aagaacuccc aggcucacca gccauaaaga aagguauccu acaauccaua     2220

aaaauaguag augaauuggu aaaaguaaug ggauacgcau uagaaucuau aguuaucgaa     2280

auggcaagag aaaaucaaac aacagcacgu ggaaagaaaa acucucaaca acgauacaaa     2340

agaaucgaag augcauugaa aaauuuagcc ccagguuuag acucaaacau uuuaaaagaa     2400

aauccaacag acaacauaca auugaaaaau gacagauuau ucuuauacua ucuacaaaac     2460

gggaaagaua uguauacagg aaaaccacua gacauaaauc aguuaucaag uuaugacauu     2520

gaccauauaa uuccucaagc cuucauuaaa gacgauucaa uagacaauag gguucugaca     2580

ucgaguaaag acaaucgugg gaaaucagac aacguaccaa gccuagaagu aguacaaaaa     2640

cgaaaggcuu uuuggcaaca acugcucgac ucaaaauuaa uaucugaacg caaauucaac     2700

aacuuaacua aagcagaacg aggaggccuu gaugaaagag acaaaguugg auucaucaga     2760

cgucaguuag uugaaacaca acagauuacc aaaaauguug cgcaaauacu agacgcacgu     2820

uuuaacacag aaguaaaaga gaaaaaccaa aagauacgca caguaaaaau cauaacgcuu     2880

aaaaguaauc ucgucucaaa uuucagaaaa gaauuuggac uauauaaagu acgagaaauu     2940

aauaacuauc accaugcuca ugacgcauau cuaaacgcug uaguagcuaa agcaauacua     3000

aagaaauauc cgaaauuaga accagaauuu gucuacggag acuaucagaa auaugaccuu     3060

aaaaaguaua ucaguagauu caaaccaucc aaagaaauag aaaaagcaac agaaaaauac     3120

uuuuucuaua gcaacuuacu aaacuuuuuc aaagaagaag uacuauacgc agauggaacc     3180

auccguaaac gcgaaaauau agaauauuca aaggacacag gggaaauugc augggauaaa     3240

gaaaaagacu uugcgacaau uaaaaaggua cuaagcuauc cgcaaguaaa cauaguaaag     3300

aaaagagaag uccaaaccgg cggauuuucc aaagaaucaa uccugccaaa aggcaacucu     3360

gacaaauuga uuccacgcaa aacaaaagau auuuuauggg acacaacaaa auauggggga     3420

uuugauucgc cugugauagc uuacucaauc uuacucauag cagauaucga aaaagguaaa     3480

gcaaagaaac uuaaaacagu gaaaacucuc guaggcauaa ccauaaugga aaaagccgcc     3540

uucgaagaaa auccaauaac auucuuagaa aacaagggcu accacaaugu cagaaaagaa     3600

aacauacuau gcuuaccaaa auauagccuu uuugaacuag aaaacggacg aagaagacua     3660

cuagcgagcg cuaaagaacu ucaaaagggc aaugaaauag uacuaccugu augucuaaca     3720

acauuauugu aucauuccaa aaaccuacac aaacucgacg aacccgaaca ccuagaauac     3780

auacaaaaac aucgcaacga guucaaagau uuacuuaacu uagucuccga auuuagccag     3840

aaguacauau uagccgaagc aaaccuagag aaaauuaaag aucucuacgc cgauaaugaa     3900

caagcugaca uagaaauucu agcaaacagc uucauaaacc uuuuaacuuu uaccgcauua     3960

ggagcacccg cagcuuucaa auuuuucgga aaagacguag auagaaaacg auauacuaca     4020

gucucagaaa uccugaaugc aacguuaaua caccaaucga ucacuggauu auaugaaacg     4080

agaauagacc uuucgaaauu aggcgaagaa uga                                  4113


<210>  5455
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5455
augaagaaac cauacucaau uggacucgac auagggacaa auuccguagg augggcaguu       60

auaacagacg auuauaaagu gcccgccaag aaaaugaaag ugcuaggaaa cacagauaaa      120

aauuauauua agaaaaaucu acucggagca cuuuuauucg auagcgguaa uacagcagaa      180

gaucgcagac uaaaaagaac agcgagaaga cgcuauacga ggagaagaaa ucgauuauua      240

uacuuacaag auaucuuuuc cgaugaaaug aacaaagugg augaaucauu uuuccacaga      300

cuagaagaca guuuccuagu auccgaagau aaaagaggag accaucaucc aauuuuugga      360

aacguugucg acgaaguuaa auaccaugaa caauucccaa caauuuacca ucuacgaaaa      420

uauuuagcag auacaccuga aaaagcagau uuaaggcuag uuuaccuagc gcucgcacau      480

augauaaaau uccgaggaca uuuucuaaua gacgacccga aacuagaugu caaaaacaca      540

gacagccaag aacuguuuuc acaauuuuua acagcauaug acguaacuca agaagauagu      600

cauuuagcag acguuaaaau aaacauuuca gacauaauaa cagagaaaau gaauaaaucu      660

agaaaaguag aagcacuacu cuuucacuuc ccaagccaga agaaaaauuc cuuuuucgga      720

aaucuaguau cccuaguuuu cggacuauua ccaaacuuua aaacaaauuu caaccucgca      780

gaagacgcaa aauuacaacu cucuaaagau ucauaugacg aagaucucga aaaccuacuc      840

ggccagauag gagaugaaua uacugacguc uucguugcag caagaaaacu auacgacucu      900

auccuuuuau cagguauccu cacaguaaau gaucuuagca caaaagccau acuuuccgca      960

ucuaugguua gaagauauga agaacauaac aaagaccuag caaaacucaa agcauucgua     1020

aaagcaaacu uuacucacga cguauuuaaa gaaguauuuu cugaugcauc uaaaaacgga     1080

uacgcuggau auauagaagg aaaaaccuca caagaagauu ucuacaaaua cgucaaaaac     1140

cuaauaggca aaguagaugg aagugccgac uucaucgaga aaauagauag agaagauuuc     1200

cuuagaaaac aacgaacauu ugacaaugga ucaauuccac aucaaguaca ccuagcugaa     1260

augaaagcaa uacucaggcg acaaggcgaa uauuaucccu ucuuaaaaga acacgccgag     1320

aaaauacaac aaauucuaac uuucaggauu ccuuauuacg uuggcccauu agauagacac     1380

ggucauucug acuuugccug ggcuuccuuc aauucggaag aaaaagugac accauggaac     1440

uuugaagaaa uuauagacaa aaacaaauca gcacaagccu ucauagaacg aaugaccaau     1500

caagaccuau accuuccuaa cgaaaaaguu cuuccaaaac acaguccacu guaugaaaaa     1560

uacacaguuu auaacgaauu aacgaaaaua aaauacguca cugaaacagg caccgcaaaa     1620

uucuuugacg cuaaccuaaa acaagaaaua uuugacggcc uauuuaagaa agaaagaaaa     1680

guaacgaaaa gaaaacuauu gacauuccua gagaaaaauu ucgacgaauu ucgaaucguu     1740

gauauucaag gucuagauaa agaaacugaa acauuuaaug cauccuacgg aauauaccaa     1800

gaucuauuaa aaauaauaaa agacaaagca uuuauggaug acucugagaa ugcugacauc     1860

uuagaagaca ucguacuaac auuaacucua uucgaagaua gagaaaugau aaaacaacgc     1920

uuggccaaau acgcugaucu auucgauaaa aagguaauug acaaucuggc aagacggaga     1980

uacaccggau ggggaagauu gucagcuaaa cuccucaacg gcauacgcga caaaagaucc     2040

agaaaaacca uaauggacua ucucguagau gacggacgau caaauagaaa uuuaaugcaa     2100

cucauaacag augacaaucu aacauuuaaa gacgaaauag ccaaagcuca auauagugac     2160

aauagcgaug acuuacauca aguaguucag gauuuagcag gcucacccgc aaucaagaaa     2220

gguauucucc aaagcguaaa aauuguagau gaacugguga aaguaauggg cuacgaacca     2280

gaacaaauag uaguagagau ggcgagagaa aaccaaacga ccgcaaaagg caggcgaaac     2340

agccaacaac gauauaaaag acuugaagaa gcaaucaaaa auuuagauag agaucuaaac     2400

uauaaaaucc ucaaagaaca cccaaccgau aaucaggcuu uacaaaauga ccgauuauuc     2460

uuauacuauu uacaaaacgg acgggauaug uauacaggag agucccuuga uaucaaucgu     2520

uugucggauu acgauauaga ccacaucaua ccacaggcuu ucaucaaaga caacuccaua     2580

gacaaccgag uccuaguauc aagcgccaaa aacagaggga aaucugauga uguuccaucu     2640

gaagauguug uaaauagaau gcguccauuu uggaauaaac uacucaauug uggacuuauc     2700

ucacaaagaa aauacagcaa ucuaaccaag aaagaacuca caccaaauga caaagcgggc     2760

uucauuaaaa gacaacucgu agaaacacgg cagaucacua aacauguggc caggauucuc     2820

gacgaacauu uuaacaagga auuugacgaa gauaagaaac gcauccgcaa aguaaaaguc     2880

guaacauuaa aaucgaauuu aguuucaaac uuccgaaaag aguucgaacu auacaaggua     2940

cgagaaauaa acgacuacca ccacgcacac gacgcguacc ucaaugcagu aguagcuaaa     3000

gcccuacuua ugaaauaucc aaaguuagag ccugaguucg uauauggaga auacccuaaa     3060

uacaacucau acagagaaag aaaaacugca acucaaagaa uguucuucua uucaaauauu     3120

augaauaugu ucaaaaguaa agugaaacuu gccgacgaaa gaauaauuga gcgaccaaug     3180

auagaaguaa acgacgaaac aggagagauu gcaugggaua agacaaaaca uauagcaaca     3240

guaaagaaag uacuuucuua uccucaaaua aauauaguca agaaagucga ggaacaaaca     3300

ggaggguuaa caaaugaaac gauauacccc cgaggcagcu ucgauaaacu ccuaaaccga     3360

aagaacacac uagaagcaag caaauacggc ggauucggaa gucccacagu agcauacugu     3420

guccuaguag uggccgacuc caaaaacgag aacggcaaag ucaagaaacu caaauuagga     3480

ucagaacuac uagggaucac aauauuagag aaaaaugacu uugaaaauaa uagccuagau     3540

uuccuuucac guaaaggaua ccaaggaguc caagagaaga aaauaauaaa acuaccuaaa     3600

uacucucuuu ucgaguucca agacgguucu cgacgaaugc uaaguagcgu cauggcaaau     3660

gacaauaaac gaggugaccu ucagaaagcu aacgaaauga uacuaccagc uuaucuagua     3720

aaacuacucu aucaugccaa acauauaaac aguguaggug aaccagcuca caaaaacuac     3780

auaaacgaac acugcuccga auuucaggau uuguugaauu acauccgaca auuugcugac     3840

aaauucguag acguagacaa aaacuuaucc aaaauaagau cucugagcaa auccauggcc     3900

gaauacucug uaucugagau uugcagcuca uucguaaauc uucuuacguu aacggcauua     3960

ggugcaccgg ccgacuucaa guuuuucgga acuacaauac caagaaagag auauggcagu     4020

ccucaaucaa uccucagugc aacccuaaua caccaaucaa uuacaggauu auacgaaacc     4080

agaauagauc uauccaaauu aggagaggac uga                                  4113


<210>  5456
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5456
augaacaaac cauacaguau uggucucgac aucggcacua acucaguagg auggaguauc       60

auaacagaug acuacaaagu uccagccaag aaaaugagag uauuaggcaa uacagacaaa      120

gaauauauaa agaaaaaucu uaucggagcc cuacuauuug acggaggcaa uacagcagca      180

gacagacgac uuaaacgaac ugcaaggcga agauauacaa gacgaaggaa uagaauauua      240

uaccuacaag aaauuuuugc cgaggaaaug ucgaaaguug acgacagcuu cuuucacaga      300

uuagaagacu cauuucuagu agaagaagac aaaagaggau cgaaauaccc aaucuuugca      360

acaaugcaag aggaaaaaua cuaucacgaa aaauucccaa cuauauauca ucuucguaaa      420

gaauuagcgg auaagaaaga aaaagccgac uuacgccuaa uauaccuugc gcuugcccac      480

auaauaaaau uucgaggcca cuuccuaauc gaggaugaca gauucgacgu acggaauacc      540

gacauucaaa aacaauauca agcauucuua gaaauauucg auaccacauu ugaaaacaau      600

gaccuauuaa gucaaaaugu agauguagag gcaauacuaa cagauaaaau cagcaaauca      660

gcgaagaaag aucguauacu agccagauau ccuaaccaga aaucaaccgg aauauucgcc      720

gaguuccuga aacuaauagu uggaaaccaa gcagacuuca agaaacacuu caacuuagaa      780

gacaaaacac cccuucaauu ugcaaaagau agcuaugaug aagauuuaga aaaccuccuu      840

ggucaaauag gagacgaauu ugcugaccuc uucucggcug caaagaaauu auacgauucg      900

guauuacuau caggcauacu aaccguaacg gacuuaagua caaaagcacc gcuaucggcc      960

ucaaugauac aaagguacga ugaacacaga gaagaccuua aacaauugaa acaauuugua     1020

aaagcgucau uaccagaaaa auaccaagaa auauuugcug acucaaguaa agacggauac     1080

gcuggauaca ucgaaggaaa aaccaaccaa gaagccuuuu auaaauaccu aucaaaacua     1140

uuaacaaaac aagagggaag cgaauaccuc uuagagaaaa ucaaaaauga agacuuuuua     1200

aggaaacaaa gaacauucga uaacggaucg auuccacacc agguacaucu uacugaacua     1260

agagcuauaa uuagacguca aucagaauau uacccguuuu ugaaagaaaa ccucgauagg     1320

auagagaaaa uauuaacauu ccggauaccu uauuauguug guccucuagc acgagaaaaa     1380

ucagacuuug ccuggaugac acgaaaaacc gaugacucua uacgaccuug gaacuuugaa     1440

gaccugguag acaaagaaaa aucggcugaa gcauucauac acagaaugac aaacaaugac     1500

uuauaucucc cagaagaaaa aguacucccu aaacauucac uuaucuauga aaaauucacu     1560

guguauaacg aacucacaaa agugcgauuu cuugcagaag gauuuaaaga cuuccaauuu     1620

uuaaaccgca aacaaaaaga aacgauauuu aauucauuau ucaaagaaaa acggaagguc     1680

accgaaaaag acauaauuuc uuuucuaaac aaagucgaug gauacgaggg gauagccauu     1740

aagggcauag aaaagcaguu caacgcuuca cucucgacau accaugaccu aaagaaaaua     1800

cugggaaaag acuuucuaga caacacagau aaugaacuca uacuagagga caucguacaa     1860

acccuaaccc uauuugaaga ccgcgaaaug aucagaaaga gauuagacau auacaaagau     1920

uucuuuacag aaucacaacu gaagaaacuu uacagaaggc acuacaccgg uuggggaaga     1980

uuauccgcca aauuaauaaa cgggauucga aacaaagaaa accagaaaac uauucucgau     2040

uacuuaauag augacggauc ggcaaaccga aacuucaugc aauuaauuaa agacgcagga     2100

uuauccuuua aaccgauaau agauaaagca agaacuggau cacacucaga caaucuuaaa     2160

gaaguaguag gugaauuggc uggauccccu gcaaucaaaa agggaauacu ucagucacuc     2220

aaaauaguug augaacucgu caaaguuaug ggauacgaac cugaacaaau aguuguagaa     2280

auggcccgag agaaucagac uacagcgaaa gguuuaucuc gccuuagaca acgacuuaca     2340

acccuucgag aaucuuuagc aaaccugaaa agcaauauac uagaagagaa gaagccaaaa     2400

uacguaaaag aucaaguaga aaaccaucau cuaagcgacg acagacucuu ucuauacuac     2460

cuacaaaacg gcaaagauau guacacagau gaugaauuag acaucgauaa cuugagccaa     2520

uacgauauag accauauuau accacaagca uucauaaaag augacucaau cgauaacagg     2580

gucuuaguca gcucagccaa aaacagaggc aaaucagacg acguuccauc cguagaaauc     2640

gucaaagacu guaaaguguu uuggaagaaa cuccuagaug caaaauuaau gagucaacgc     2700

aaauaugaca auuuaacaaa agcagaacgu ggcggcuuaa cgucagacga uaaagcccga     2760

uucauccaga gacaauuggu ugaaacuagg caaaucacua aacaugucgc acgaauauua     2820

gaugaaaggu uuaacaacga auuagacuca aagggcagac gaauaaggaa aguaaaaaua     2880

gucaccuuaa agagcaacuu aguuaguaau uucagaaaag aguucggauu cuauaagauu     2940

agagaaguaa acaacuacca ccaugcgcau gacgcuuacu ugaacgcugu cguugcaaaa     3000

gcgauucuua cuaaauaccc acagcucgaa cccgaauuug uauacggcga uuacccaaaa     3060

uacaacucau acaaaacacg aaaaagugcc acagaaaaac uauucuuuua cucgaauauu     3120

augaauuucu uuaaaacuaa aguaacauua gcagacggaa cuguuguagu aaaagaugau     3180

auugaaguua acaacgacac aggagagaua guaugggaua agaagaaaca cuuugccacc     3240

guucgaaaag uauuaucaua cccacaggua aacauuguaa agaaaacuga aauacaaaca     3300

gggggcuuua gcaaagaguc aauacuagca cacggaaauu cggauaaauu aauccccagg     3360

aaaacuaaag auaucuaccu cgaccccaag aaauauggug gauuugauuc accaauuguc     3420

gcauauucag uauuaguugu cgccgauauc aaaaagggua aggcccaaaa acuaaaaacu     3480

guaacagaau ugcuuggaau aacaauaaug gaacgaagua gauucgagaa aaacccauca     3540

gcuuuccuag aaucuaaagg auaucucaau auuagggacg auaaacuaau gauuuuaccu     3600

aaauauagcc ucuucgaauu agagaacgga agaagaagac uauuagcauc cgcagacgag     3660

cuacaaaaag gcaacgaacu agcccuaccu acccaauuua ugaaauuccu guauuuagca     3720

aguagauaca augaauuaaa aggaaaaccg gaagaaauag aacaaaaaca agaguuugua     3780

guacaacacg ucuccuauuu cgacgauaua cuacaaauaa uuaacgacuu caguaaucgu     3840

gucauacuag cagaugccaa cuuagagaaa auuaacaagu uauaccaaga uaacaaagaa     3900

aauauccccg uugaugaacu agcaaacaac auaauaaacc uuuucacauu uacaagucua     3960

ggagcccccg cagcauuuaa auucuuugac aaaauaguug acagaaaacg uuauacauca     4020

acaaaagaag uauuaaacag cacucugauc caccaaucaa uuacaggccu uuacgaaacc     4080

agaauagauc ugggaaaacu aggagaagac uga                                  4113


<210>  5457
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5457
auggaaaaaa acaaggaaua ugucauaggg uuagacauag gcaccggauc aguuggaugg       60

agcgcaauau cuacagauaa cuaccaacua aucagacaaa aaggaaaaaa uuuaauuggc      120

guacgauuau uuaauucagc ugaaacagcg gaagaaaggc ggggauauag aacgacgcgu      180

agaagacuaa guagaaggag auggaggaug agccuuuuaa acgauauauu uagaccagaa      240

cuuaacgaaa uagacgagaa uuuuuuagca agacuaaaau auuccugggu aagcccccuu      300

gaccaaaaaa auccacagcu aaacgaacaa ggaaccaaug caucacucua uggagauaua      360

gaggcggauc aagcguuuca ucgggcauac ccaaccauuu accaccuacg aaaaacacuc      420

auggaagaca aucaaaaaca cgaccuaaga gaaguauauu uagcgauaca ucauauuguu      480

aaauaccgag gacacuuccu gacucccgau uuugacgcua auagucaaac gauagauauc      540

aaaggauuau uuaaacagcu uggagacgug ucagggucga uagaauuaca agcagaucaa      600

accucuacag uaauagcugc cuuucaaucc auauuguuag acuucaccaa gacaccaacc      660

gaaucaggca gacuagcagg ccaacucuuc ucagaccaga auaaggauaa acaggccaaa      720

aagcaagucg aagaguuguu caaacuauua agaggaggga aaggauccuu agcggacaug      780

uuccuccuua cagaccuaga uaaagugguu aaaaaucaac uuaaauucgc auucgaugau      840

gcaguagaug agaaucuuaa cuuaauaucu aaucucauaa acgaagaaca auacgagcuu      900

uuacucaaca uacagaaacu cagaggcaca aucauacuua aacaaauauu aggagacgac      960

gaaacaguau cagaugcaaa aguccgcagu uaugaggaac acgcucauca acuaaaagaa     1020

cuaaaggccc aaguacuaac uaaagauaau aaagaaaaau ucgaagaagc auaccaauua     1080

auacaaaaag gcgaugcaag caggaucgau ggucucaaaa aguuaaaaac aaucauaaac     1140

caagaacacg aagcucuaag acuagauaaa uacgaagcag auaaauuacu aaaauugauc     1200

gaacuagacc aacuauuacc uguacagaga ucgaaacgca acggcgaaau cccacaccaa     1260

cuacacgaau uugaauuaaa aaaaauaaua gaaaaacagg guagauauua cccguuccuu     1320

agagaaacau uugaacgcga cggacaagag uucaacaaaa uaggccaacu uguuaacuuu     1380

agaauaccuu acuacguagg accguuagua gacccaaggc ucgcugguau ccagucgggg     1440

gaucaaacaa aucacuggau gacacaaaaa aaagcaggaa aaauuacacc auggaacuac     1500

acugagcuca ucgauauuga uaaaagcgca gcagaauuca uaaaaagaau gacagguaca     1560

gauacguauc uaguaggaga accaucauua cauaaagcaa gucucauaua ccaagacuac     1620

aauguacuac aagaauuaaa caaccucaga uacgacggca aaccacuucc aguagacgca     1680

aagcaagaaa ucuacucaaa ccuacaaguc agcuuaaaaa augcaaccaa aaauaaaaua     1740

aauucauucc ucaucuccga aggauaucua caagacggug aagaacuuac aggauuugcc     1800

ggcaaagaau uaaaaaauaa acuaagcagu uuccacuacu ucaaaaacau auucggaccu     1860

gaacgaauga acacauuagg uaccgacaac uuggaaaaaa ucacagagcu acaaaccgua     1920

uuugaagaca gacauaugcu aaaaagacaa cuucagaaac uagguuuccu agacgacgac     1980

gaaugugaaa aauuagcagg aaaacauuuc acuggcuggg gcaaucucag caaaaaauua     2040

cuaaauacac cggcgaucau agacccagaa auaaacgaga auaaaauaag uuuaaucgau     2100

gcauuauaua aaggacaaag aaaccucagu uuuuacguaa auaacaugga cgacaaauau     2160

caauuuaggg acuggauaga gucagaaaac caaagccaac aaguagaaau aucccuggau     2220

gaaaaaauua aaaauuuggc aggaagucca gcuaucaaaa gagggaucac acaaagcaug     2280

gcaguuauac aagauauaau uagggcaaau aacggaauau caccaaaaag aauuuaccua     2340

gaauucgcga gggaaucaca aaauaguuau caacccaauu cuagaauuaa acaaauagaa     2400

aaaauauacg aucaaaacaa aaaagaacua augaaagucu acccugacgu auuuaaggac     2460

guucaagggg uauuaaauaa ugucgacgaa aauaacuccu uaucagaaaa agcauuccug     2520

uacuucaugc aacuuggaaa agauauguau acaggugaag cauuggacuu cgauaaauua     2580

uauacauaug acaucgacca uaucauccca caagcaauga cuaaagacaa cagcugggac     2640

aauaaaguau uagucacagc cauaaaaaac cgagagaaau caaaccaagu acucgauaac     2700

gcaauacaag caaaaaugca caauuucugg cuucaacuaa aaaagguuaa cuuacucuca     2760

cagaggaaau augacacauu aaugaaaaca aauuucagca agggagauga aaaaaaauuc     2820

auugcccgau cacuagugga aacuagacaa auaauaaaaa acguugcaga acuaauaaaa     2880

ucggaauuug accacguaga agcaauucca auuagacaag aacuuacauc agacaugaga     2940

caauuaaugg auuacaaaaa aaaucgacuu guaaacgauu aucaucacgc gcacgacgca     3000

uugcuaauug caacuguagg cgaauuuaug caaaaacgag guuucaugca aggaaugaac     3060

cccucacaaa auguuuacca cggauacaca caauauguca aaaaauggcu cuccauggca     3120

aaaaaggcca gaucagcgga aaaacccgaa agaauuuuuc cacuacaauu uguuguaggg     3180

ucaaugagaa uggcaguuag agauggacaa guggacaaag uaacacaccc cgcuacaggg     3240

gaaauaugcu ggacagcuga caacgaagcu uaucucagga aaguacuagg uuauaaaaau     3300

auuuuaguaa cacgcaaggu aaaaaacgca ggaaaacugu aucaacgagu aaccguaaag     3360

ccaaaaggau caggagaagc accaguaaac caccaaaaau cagacaucaa cauuuacgga     3420

ggagugacaa gcgaacaagc cgcugaacua guacuaauac gaauaaaagc aaagaauaau     3480

gccaaaaaua ugguaaucaa aauaccuaca gcgaucgcca accaaguugc gaacaaaacg     3540

acaacuuacg augcauacgu gagaucacuu aauauaccua acuacgccgg cauaauuuug     3600

gaucaaauuc ccuugggaca acuaguauuu gaugagagga cuucauuuua cuuagcaagu     3660

gcagaaauua aacauaacgc ccgacaacua uggguuaacc aacauauaaa uaacguacuu     3720

acgaaaaaaa auauaaacga cgaagaaaga gauaauauac uaaaccugcu aaccaacgaa     3780

aaaaaucucg aaagauuuaa acuucuagaa cccgaccuaa aaagagugaa agcugcgaaa     3840

caacaauggg caucguguga cgaucaagaa caaaugcaga uucuaagaau uauuauaaac     3900

aaaaugcacg augacgcauc cccucaaaua ccagguaaaa uagauccauu uaaaaauuau     3960

cuggauaaac aagguaaagg auggcgagac cuuaggacga aaaaaggcau gaaauuauca     4020

gauaaugcgg aauuuguuua ccaaucccca acuggacuau aucaucgcaa aauaucaaua     4080

aaugaauuac uaaaacaaaa acaacuagaa uga                                  4113


<210>  5458
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5458
augaacaaac cguacaguau aggucuugau auuggaacaa auagcgucgg uuggucaaua       60

auuacagacg acuauaaagu accagccaag aaaaugagag uacuagguaa cacggauaaa      120

gaauacauca agaaaaauuu aauuggugca uuguuauuug acggcggcaa cacugcagca      180

gacagaagau uaaaacgaac agcaagacgu cgauacacua gaagaagaaa ccguauccug      240

uaucuacaag aaauauucgc agaagaaaug ucaaaagugg acgacagcuu uuuccaccgc      300

cuagaagaua guuuucuagu ugaagaagau aaaaggggau cuaaauaccc uauauucgca      360

acgaugcaag aggaaaagua uuaucaugaa aaauuuccca caauauacca ucucagaaaa      420

gaacuagcug auaagaaaga aaaagcagau cuaagacuaa uauaucuagc acuagcacac      480

auuauaaaau uuagaggaca cuuucuaauc gaagaugauc guuuugacgu acgaaacaca      540

gauauccaaa aacaauacca agccuucuua gaaauauucg auaccacuuu cgaaaacaac      600

gaccucuugu cgcaaaaugu agacguagaa gcaauauuaa cagacaaaau auccaaaucg      660

gccaagaaag accguauucu ugcuagauau ccaaaccaaa aauccacugg aauauuugca      720

gaguucuuga aauuaauagu gggaaaucaa gcagauuuua agaaacauuu uaaccuagaa      780

gacaaaaccc cacuccaauu ugcaaaagac ucauaugacg aagauuuaga aaaucuacuu      840

ggccaaaucg gagacgaauu ugcagaucua uucucagcag ccaagaaacu auacgauuca      900

guacuguuau caggaauuuu aacaguaacg gaucuuagca caaaagcucc acuaagugca      960

ucuaugauac aacgauacga cgaacaccgu gaagaccuca aacaacuaaa acaauuuguu     1020

aaagccucgc uaccagaaaa auaucaagaa auuuuugcag acuccagcaa agacgguuau     1080

gcaggcuaua uagaaggaaa aaccaaucaa gaagcguuuu acaaauacuu aucuaaauua     1140

uugaccaaac aagaagguag ugaauacuug uuagaaaaga uuaaaaauga agacuuucuu     1200

cgcaaacaac gcacauucga uaauggaucc auaccacacc aaguacaccu aacagaacua     1260

cgugcaauaa uccgcagaca gagcgaauac uaucccuucu uaaaagaaaa ucucgaucgc     1320

aucgagaaaa uuuuaacauu cagaauacca uacuacguag guccacuagc aagagagaaa     1380

agugacuucg cauggaugac uagaaaaaca gacgacucca uaagaccaug gaacuucgaa     1440

gaccuagucg acaaagagaa aagcgcagaa gccuuuauac accguaugac gaauaacgau     1500

uuauacuuac cagaagaaaa aguacucccc aaacauucac uaauauauga aaaauucaca     1560

guauacaacg aacugacuaa agugagauuu uuagcagaag gauuuaaaga uuuccaauuc     1620

cucaaccgaa aacaaaaaga aacaaucuuu aauagucuau ucaaagaaaa acgaaaagua     1680

acagaaaaag auaucauuag cuucuuaaac aaaguagacg gcuaugaagg cauagcaaua     1740

aaaggaauug aaaaacaauu caacgcauca cuauccacau accaugaccu caagaaaaua     1800

cugggaaaag auuuccuuga uaauacagac aacgaauuaa uauuagaaga caucguucaa     1860

acuuugacgc uauucgaaga cagagaaaug aucagaaaaa gacuagauau auauaaagac     1920

uuuuucacag aaagucagcu caagaaauua uacagaagac acuauacggg auggggucga     1980

cuaucagcua aacuaaucaa cggaauacga aauaaagaaa accaaaagac aauacuugau     2040

uauuuaauug augacgguag ugcaaaucgu aauuuuaugc aacucaucaa agaugcuggu     2100

cuaucguuua aaccuauaau ugauaaagca aggaccggau cccacuugga caacuuaaaa     2160

gaaguaguag gagaacuagc aggaucacca gcaaucaaga aaggaauccu acagucccua     2220

aaaaucguag augagcuagu caaagucaug gguuacgaac cggagcaaau uguggucgag     2280

auggcacgug aaaaucaaac aacagcaaaa ggacuaucaa gauccagaca gagacuuacg     2340

acacuaagag aauccuuggc caaucuaaaa ucaaacauau uagaagagaa gaaaccaaaa     2400

uauguaaaag accaaguuga aaaucaccac cuauccgaug acagguuauu ccucuauuau     2460

cuacaaaaug guaaagacau guacacagau gaugaacuag auauagacaa ccuuagccaa     2520

uaugauauug accacauuau accucaagca uucauaaaag acgauucgau agauaaucga     2580

guacugguau cuucagccaa aaacagaggu aaaucagaug auguaccuag uuuagagaua     2640

guaaaagauu guaaaguauu cuggaagaaa cuucuagacg caaaacuuau gucccaaaga     2700

aaauaugaua aucuaacaaa agcagaaaga ggaggacuga cgagugacga caaagcaaga     2760

uuuauucaac gacaacucgu cgaaacacgc caaauaacua aacauguggc aagaauccug     2820

gacgaaagau uuaacaacga acucgauagc aaaggaagac gaauuagaaa agucaaaauc     2880

gugacacuca aaucaaaccu cguuaguaau uucagaaaag aguucggcuu cuacaaaauc     2940

cgagaaguga acaacuauca ccaugcucau gacgcauauc uaaaugcugu cguugccaaa     3000

gcgaucuuaa caaaauaccc acaauuagag ccugaguucg ucuacggaga cuaucccaaa     3060

uauaacucau acaaaacuag aaaaucugca acggaaaaac uauuuuucua cuccaacaua     3120

augaacuuuu ucaaaacuaa aguaacccua gccgacggua cugucguggu gaaagacgac     3180

auagaaguua auaaugacac uggugaaaua guaugggaca aaaagaaaca uuuugcaaca     3240

gucagaaaag uguuaaguua cccucaaguc aacaucguca agaaaacaga aguucagacc     3300

gguggcuuuu cuaaagaguc gauauuagcc cacggcaaua gcgauaaacu aaucccaaga     3360

aaaacuaaag acauauaccu agaccccaag aaauacggag gauucgauuc accaauagua     3420

gcauauagug uacuuguugu ugcugauauc aagaaaggga aagcacaaaa acugaaaacc     3480

guaacugaac ugcuaggaau cacuauaaug gaaagaucaa gauuugagaa aaauccuagu     3540

gcauuuuuag aaucaaaggg uuaucucaac auaagaacag auaaauuaau aauacugccc     3600

aaguacagcc uuuucgagcu ugaaaacgga cguagacgcc uacuugcuuc agcaggagaa     3660

cugcaaaaag gcaacgaacu cgcacuaccu acucaauaua ugaaauuucu auacuuagca     3720

agucgcuaua acgaacuuaa aggaaagccc gaagaaauug aacaaaaaca agaguucguc     3780

guucagcaug uaagcuauuu cgacgacauc uuacaaauaa ucaacgacuu uucaaaucgc     3840

guaauauugg cagacgcaaa ucuagagaaa auaaauaaac ucuaucagga caacaaagaa     3900

aacauaccug uugacgaacu agcaaacaau auuauaaacu uauucacguu cacaucuuua     3960

ggggcaccag cagcauucaa guuuuucgau aaaauaguag acagaaaaag auauaccucu     4020

acaaaagagg ucuuaaauag caccuuaauc caccaaucaa uaacgggacu auaugaaaca     4080

cgcauagacu uaggcaaacu aggcgaagga uga                                  4113


<210>  5459
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5459
augaacaaac cauacucgau aggccucgac auaggcacaa auagcguggg uuggucaaua       60

auaacagacg acuauaaagu accugcuaag aaaaugagag uacucggaaa uacugauaaa      120

gaauacauca agaaaaauuu aauaggagca cuucuauuug auggagguaa uaccgcggcc      180

gauagaagau ugaaaagaac ugcccguagg agauacacua gaagaagaaa caggauacua      240

uauuuacaag aaauauuugc ugaagaaaug ucaaaaguag acgacuccuu cuuucaucgg      300

cucgaagauu cuuucuuagu ggaagaagac aaaaggggcu cuaaauaucc aauuuuugca      360

acucuucaag aggaaaaaga uuaccaugaa aaauuuagca caaucuauca ucuaaggaaa      420

gaauuagcag acaagaaaga aaaagcagau cuuagacuaa uauauauagc auuagcacau      480

auaauuaaau uuagaggcca cuuucuaauu gaagaugacu cauucgacgu ucguaauaca      540

gacauaucca aacaauauca agacuuucua gaaaucuuca auacgacguu cgaaaauaac      600

gaucuacuuu cacaaaacgu agauguugaa gcuaucuuaa cugauaaaau uucgaaaagc      660

gcgaagaaag auaggauccu ugcacaauau ccaaaucaga aaagcacagg cauauucgcc      720

gaguucuuaa aacuaaucgu ugguaaucaa gcagauuuca agaaauacuu caaucuagaa      780

gauaaaacac cccuccaauu cgcaaaagac aguuaugacg aagacuuaga aaaccuacuu      840

ggccaaauag gagaugaauu ugcagaucua uuuucugcag ccaaaaagcu auaugacagc      900

guauuauuau caggaauauu aacaguuaua gauuuaucaa caaaagcucc ccuaagugca      960

aguaugaucc aacgauauga ugaacauaga gaagaccuaa aacaacuaaa acaauuuguu     1020

aaagcaagcc uaccugaaaa auaccaagaa auauuugcag acaguucuaa agacggauau     1080

gcuggauaca uagaaggcaa aacaaaucaa gaggcguucu auaaauaucu guccaaacua     1140

cucacaaaac aagaagacag ugagaauuuc uuagagaaaa uuaaaaacga agauuuccua     1200

cggaaacaac gaacauuuga caacggaagu auaccacacc aaguucaucu uacggaacua     1260

aaagcuauca uaagaagaca auccgaauau uacccauuuu uaaaagaaaa ucaagauaga     1320

auugagaaaa uacuaacguu caggauuccc uauuacauag guccacuggc aagagagaaa     1380

ucagauuuug cauggaugac aagaaaaacu gaugauagca uacgaccgug gaauuucgaa     1440

gaucuuguag acaaagagaa aagcgcugaa gcauuuauac auagaaugac gaauaacgau     1500

uucuaccucc cagaagaaaa agugcucccc aaacauuccc uuaucuauga aaaauuuaca     1560

guauauaacg aacucacgaa aguaagauau aaaaacgaac aaggcgaaac auacuuuuuc     1620

gauucaaaua ucaaacaaga aaucuucgau ggagucuuua aagaacauag gaaaguuagc     1680

aaaaagaaau uauuagacuu cuuggcaaaa gaauacgaag aauuuagaau cguagacgua     1740

auuggacucg auaaagaaaa uaaagccuuu aaugcaagcc uagguacaua ucaugacuug     1800

gagaaaauuu uagauaaaga cuuccuagau aacccagaua augaaucaau auuagaagau     1860

auaguacaaa cccuaacccu auuugaagac cgugaaauga ucaagaaaag acuugaaaac     1920

uacaaagacc uguuuacaga aucacaacuu aagaaacuuu acagaaggca cuacacagga     1980

ugggguagac uauccgcuaa acuaauaaau ggaauuagag auaaagaauc ccaaaagacc     2040

auauuagauu aucucauaga cgacgguaga ucgaauagga auuucaugca auugaucaau     2100

gacgauggau uaaguuucaa aagcauaaua ucaaaagccc aagcaggguc acauucggac     2160

aaccuuaaag aaguagucgg agaacuagcc gggucacccg caauaaagaa aggaauacug     2220

caauccuuaa aaaucguaga cgagcuaguu aaaguaaugg gauaugaacc cgaacaaauu     2280

guagucgaaa uggcacgaga gaaccaaaca acaaaucaag guagaagaaa cagcagacaa     2340

cguuauaaau ugcuugacga uggagucaaa aaccuagcau ccgaccuaaa cggaaauauc     2400

cuaaaagaau acccaacaga uaaucaagcg uuacaaaaug aaagacuauu uuuauacuac     2460

uugcaaaacg ggagagacau guauacaggg gaagcgcuag acaucgacga uuuaucacaa     2520

uaugauaucg aucacauaau cccccaggcu uuuauaaaag acgacucuau agauaacaga     2580

guucuaguau caucagccaa aaaucggggc aaaucggacg auguuccauc ccuagaaauu     2640

guuaaagacu guaaaguauu cuggaagaaa cuccuagacg caaaacugau gagucaaaga     2700

aaauacgaua acuuaaccaa agcagaacga ggaggacuaa ccucagauga caaagcaaga     2760

uucauucaaa gacaacuugu agaaacgaga cagauaacaa aacaugucgc caggauacua     2820

gacgaaagau uuaacaacga auuagauucg aaaggaagaa gaauaagaaa aguuaaaauc     2880

gucacacuaa aaaguaauuu aguuucaaau uucagaaagg aauuugguuu uuauaaaauc     2940

cgagaaguua acaauuauca ucaugcccac gacgcauacc uaaacgccgu gguagcaaaa     3000

gcaauauuaa caaaauaccc ccaauuagaa ccggaauuug ucuauggaga cuauccaaaa     3060

uacaauucau acaaaacuag aaaaagcgca acugaaaaau uauucuuuua uaguaacauu     3120

augaacuucu uuaaaaccaa aguaacccua gcagauggaa caguagucgu caaagacgau     3180

auagaaguaa acaaugauac cggagaaauc guaugggaca agaagaaaca uuuugcaaca     3240

guacgaaaag uucuauccua uccucaaaac aauauaguca aaaagacaga aauacaaaca     3300

ggaggcuuuu cuaaagaauc aauucuagca cacgguaacu cagauaaacu aaucccccgu     3360

aaaacaaaag auauauaccu agauccuaag aaauacggcg gcuucgauag ucccauuguc     3420

gcauacucag uacugguagu agcagauauc aagaaaggaa aagcacaaaa auuaaaaacu     3480

guuacagaac uauuaggaau cacuauaaug gaaagaagua gauucgagaa aaauccauca     3540

gcauucuugg aaaguaaagg cuauuugaac auaagagcag acaaguugau cauauuaccc     3600

aaguacuccu uauucgagcu agagaauggu cgcaggcgau uacuagcgag cgcuggcgaa     3660

cuacaaaagg gcaaugagcu ugcacuacca acucaauuca ugaaauuucu auaccucgca     3720

agccguuaua acgagucaaa aggaaaaccg gaagaaauag agaagaaaca agaauuugua     3780

aaucaacaug uuuccuacuu cgaugacaua cuacagcuca uaaacgacuu uucuaaacgu     3840

gucauacuag cagaugcaaa ucuagaaaag auaaacaaac uauaccaaga uaauaaagaa     3900

aacauaucgg uagacgaacu agcaaacaac auaauuaauc uguucacauu cacaucacua     3960

ggagcgccug cagcuuucaa auuuuucgac aaaauaguag acagaaagag auacacauca     4020

acaaaagaag uauuaaacuc aacgcuaauu caucaaucaa uaacagguuu auacgaaaca     4080

cgcauagacu uaggaaaacu aggagaagau uga                                  4113


<210>  5460
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5460
augaacaaac cguauucuau aggauuagac auaggaacaa auaguguugg uugguccaua       60

auuacagacg auuacaaagu accagcgaag aaaaugagag uacucgguaa cacggacaaa      120

gaauacauua agaaaaaccu uauaggagca uuauuauucg auggaggcaa uacagcugca      180

gaccgacgac uaaaaaggac cgcaaggcga agauacacca gaagaagaaa ccgcauauua      240

uacuuacaag aaauuuuugc cgaagaaaug agcaaaguag acgauucauu cuuccauagg      300

cuugaagaua gcuucuuagu agaagaagac aaaaggggaa guaaauaucc caucuucgcc      360

acauuacaag aagaaaaaga uuaccacgaa aaauuuagca caaucuacca ccuaagaaaa      420

gaauuagcag acaagaaaga aagagcugau cuacgauuaa uauacaucgc ucuagcgcau      480

auaauaaaau uuagaggaca uuucuuaauc gaagacgaca guuuugaugu aagaaauacu      540

gauauaagua aacaauauca agacuuuuua gaaaucuuca acacuacuuu ugaaaauaau      600

gaucugcuca gccaaaacgu agauguagaa gcaauacuaa ccgauaaaau cucaaaaucu      660

gccaagaaag aucgcauauu agcccaauac ccaaaccaga aaucuaccgg aaucuuugcu      720

gaauuuuuaa aauuaauagu agguaaucaa gcugacuuca agaaauauuu caauuuagaa      780

gacaaaacuc cacuucaguu cgcuaaagau ucauaugaug aagaucuaga aaaccuauua      840

ggucagauug gagaugaguu cgcagaccuc uucucugcgg ccaagaaacu auacgacagc      900

guauuauuau caggaauauu aacaguaaua gaucuaagca ccaaagcacc cuuaucugca      960

ucaaugauac aacgauauga ugaacacaga gaagaccuaa aacaauuaaa acaauuugua     1020

aaagccucac uaccugaaaa auaccaagaa auauuugccg auucaagcaa agacggcuau     1080

gcaggauaua uugaagggaa aacaaaccaa gaggcuuucu acaaauacuu aucuaaauua     1140

uuaaccaaac aggaagacuc ugaaaacuuc uuagagaaaa ucaaaaacga agauuucuua     1200

cgaaaacaga ggacauuuga uaacggaagc auuccccacc aaguacauuu aaccgaauua     1260

aaagcaauua uuagacgcca gucagaauac uaucccuucu uaaaagaaaa ccaagaucga     1320

aucgagaaaa uuuuaaccuu uagaaucccc uauuacauag gaccucuagc acgugagaaa     1380

agugacuuug ccuggaugac aagaaaaaca gacgacagua uacguccaug gaacuuugaa     1440

gaccugguag acaaagagaa aagugcagaa gcguucauuc aucgaaugac uaauaacgau     1500

uuuuaccuac ccgaagaaaa aguacuucca aaacauaguc uaauuuauga aaaauucaca     1560

guguacaacg aacuaacaaa aguccgauac aaaaaugaac aaggagaaac auauuucuuu     1620

gauagcaaua uaaaacaaga aauauuugac ggaguguuua aagaacauag aaaagugagc     1680

aaaaagaaac uccuugauuu cuuagccaaa gaauaugaag aauuuaggau aguagacguu     1740

auuggacugg auaaagaaaa caaagcauuu aacgccaguc ucgggacaua ucaugacuua     1800

gagaaaauac uagauaaaga cuuucuagau aauccagaca acgaaucaau auuagaagau     1860

auuguccaaa cauuaacacu auuugaagac agagaaauga ucaagaaacg uuuagaaaau     1920

uacaaagauc uguucacaga aucacaacua aagaaacuuu acaggcgaca uuauaccgga     1980

uggggcagac ucucugcgaa acuaauuaau ggaauaagag acaaagaauc ucagaaaacg     2040

auacuugacu acuuaauaga ugacggaaga ucaaaccgga acuucaugca auuaauaaac     2100

gaugacgguc uauccuuuaa aaguauaaua ucgaaagcac aagcuggauc ccacagugau     2160

aacuuaaaag aaguaguagg ggaacuggcg ggaucgccag cgaucaagaa agggauauua     2220

caaaguuuaa aaauagugga cgaacugguc aaagugaugg gauaugaacc agaacaaaua     2280

guaguagaaa uggcccgcga aaaucaaaca acuaaucaag gaagacgaaa cucgagacaa     2340

agauauaaau uacucgacga cggggucaaa aauuuagcga gcgaucuaaa uggaaacaua     2400

uuaaaagaau accccacaga caaucaagca cuacaaaacg aacgucucuu ucuguauuac     2460

cuccaaaaug gaagagauau guacacagga gaagcucuag acauagauga ucuuucgcaa     2520

uaugacauag accauauuau accacaagca uucauuaaag augacagcau agauaauaga     2580

guauuaguau caagugcuaa aaaucggggu aaaagcgacg acguccccuc ucuagaaaua     2640

guaaaagacu gcaaaguauu uuggaaaaag cuauuagaug caaaacuaau gucacaacga     2700

aaauaugaca aucugacaaa agcagaaaga ggagggcuca ccucagacga caaagcaaga     2760

uucauacaaa gacaauuagu ugaaacuaga caaauaacaa aacacguugc cagaauauug     2820

gacgaaaggu uuaauaauga auuagacucc aaagggagaa gaaucagaaa aguaaaaaua     2880

guaacacuaa aauccaacuu agucaguaac uucagaaaag aauuuggauu uuacaaaauc     2940

cgagaaguaa auaauuacca ccaugcccac gacgcuuacc uaaaugcggu uguagcaaaa     3000

gcaauuuuaa cuaaauaccc acaacuugaa cccgaauuug uauauggaga cuauccaaaa     3060

uacaauaguu auaaaacacg gaaaagugca acagaaaaac ucuucuucua cagcaacauc     3120

augaauuucu ucaaaacgaa aguaacgcuc gcugacggaa caguugucgu aaaagaugau     3180

auagaaguaa auaaugacac uggugaaaua guaugggaua aaaagaaaca cuuugcuaca     3240

guacgcaaag uacugucaua uccacaaaac aauauuguga agaaaacaga gauucaaacc     3300

ggaggauuuu cuaaagagag uauuuuagcu cauggcaacu ccgauaaacu aauuccaaga     3360

aaaacaaaag acauauaucu cgauccaaag aaauacggcg gguucgacag uccuauugua     3420

gcuuauuccg uauuaguagu ugcagacauu aagaaaggaa aagcacaaaa auugaaaacc     3480

guaacagaau uacuaggaau aacaauaaug gaaaggucgc gcuuugagaa aaacccauca     3540

gccuuuuuag aaucgaaagg auaucucaac auccgcgcug acaaauuaau aauacuucca     3600

aaauacucac uauucgaguu agaaaauggu agacgacgcu uacucgcauc agccggugaa     3660

cuccaaaaag gaaacgaauu ggcgcugccu acacaguuca ugaaauuuuu guacuuagca     3720

ucccgauaca augagagcaa agguaaacca gaagaaauug agaagaaaca agaguucgug     3780

aaccaacaug ucucauacuu ugacgauaua cuccaauuaa uaaacgacuu uucuaaacgu     3840

gucauauuag cagacgcaaa uuuagagaaa auaaacaaau uauaucaaga uaauaaagaa     3900

aauauaucag ucgaugaacu agcaaauaac auaaucaacc uauucacauu cacaagucuu     3960

ggcgcuccag cagcauucaa auuuuucgac aaaaucgugg auagaaaaag auacacaagc     4020

acgaaagaag uucuuaauag uacauuaauc caccagucaa uuacuggauu auaugaaacu     4080

agaauagauc uaggaaaauu aggcgaagac uga                                  4113


<210>  5461
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5461
augaacaaac cuuacaguau agguuuagau auaggaacua acagcguggg auggucaaua       60

auaaccgacg acuauaaagu accagcuaaa aaaaugcggg uauuagggaa uacagauaaa      120

gaauacauaa aaaaaaaccu aauaggagcu uuguuauucg acggcggaaa uacagcuuca      180

gaccgaaggc uuaaaagaac agccagacga cgauauaccc guaggcggaa uagaauauua      240

uacuuacaag aaauauucgc ugaagaaaug ucaaaaguug acgacucauu cuuccaccga      300

cuagaagaca gcuuccuagu agaagacgac aaaagaggau ccaaauaucc aaucuucgcu      360

acaaugcaag aggaaaaaga cuaucaugaa aaauucccca caauauauca cuugcgcaaa      420

gaacuagcag auaagaaaga gaaagcagac cuucgucugu uuuauuuagc auuagcucau      480

auuaucaagu uuagaggaca cuuuuuaaua gaagacgacu ccuuugaugu gagaaauaca      540

gacauccaac gucaguauca agccuucuug gaaauauucg auacuacguu cgaaaauaac      600

caucuauuau cacaaaacau agauguugaa ggaauccuaa cagacaaaau aagcaaaucc      660

gcaaaaaaag aucggauccu cgcacaauac ccaaaccaaa aauccacugg cauuuucgcc      720

gaauuucuaa aauuaaucgu ggguaaccag gcagauuuca aaaaacacuu caauuuagaa      780

gacaaaaccc cacuacaauu cgccaaagac ucauacgaug aagaccucga aaaccucuua      840

ggacaaaucg gcgacgaauu ugcugaccug uuuucaguug caaaaaaacu auacgauagu      900

guauugcuuu cugguauauu aacaguaaca gaccuaucca caaaagcacc cuuaagugca      960

ucgaugauac agagauauga cgagcauaga gaagaucuaa aacaauuaaa acaauuuguu     1020

aaagcuuccu uacccgaaaa auaccaagaa aucuuuaccg acucgaguaa agacggauau     1080

gcaggcuaca uagaaggaaa aacuaaccaa ggagccuucu auaaauaucu aucaaaauua     1140

uuaacaaaac aagaaggauc ugaauacuuc uuagaaaaaa uuaaaaauga agacuuucuu     1200

cgaaagcaaa gaacauucga uaauggaucc aucccacacc aaguucauuu aaccgaguug     1260

aaagccauaa uaagaagaca aucagaauac uacccauucc uaaaagaaaa ccuagaccgc     1320

auugaaaaga uauuaacguu cagaauccca uacuacguag ggccauuagc gcgagaaaaa     1380

agcgauuucg cauggaugac acguaaaacc gaugacucaa uacgaccaug gaauuuugaa     1440

gaauuggucg auaaggaagc aagcgcagaa gcauucauac accgaaugac aaauaaugau     1500

cuauacuuac cagaagaaaa aguauuacca aaacauagcu uaauauacga aaaauucaca     1560

guauauaaug aacucacaaa agucagauau aaaaaugaac aaggggagac auacuuuuuc     1620

gacaguaaca ucaaacaaga aauauucgac ggaguauuca aagaacacag aaaagugucc     1680

aaaaaaaaac uacuugauuu ccuagcaaaa gaauaugaag aauucagaau aguagacgua     1740

auaggccuag acaaagaaaa caaagcuuuu aaugccucuc uaggcacaua ccaugaucuu     1800

aaaaaaaucu uagauaagga cuuuuuagau aauccagaca acgagagcau acuagaggau     1860

auuguccaaa cccucacguu auuugaagau agagaaauga uaaaaaaaag acucgaaaac     1920

uauaaagacc uguucaccga aucacaauua aaaaaauuau accgaaggca uuacacuggu     1980

uggggacgac ucucagcaaa auuaauaaau ggaauaagag acaaggaaag ccaaaaaacu     2040

auauuagacu aucuaauaga ugaugguaaa agcaacagaa auuucaugca auuaauacac     2100

gacgacgggc uaagcuucaa aagcauuauu agcaaagcac aagccggaag ucacuccgac     2160

aaccuuaaag agguaguagg cgaauuagcc ggcuccccgg ccauaaaaaa aggaauauua     2220

cagucauuaa aaaucgucga cgaacuagua aagguuaugg gauaugaacc ugaacaaauc     2280

guuguagaaa uggcuagaga aaaucaaaca acuaaucagg gacgacgaaa uucaagacaa     2340

agguauaaac uacuagauga cggagucaaa aaccuugcua gcgauuugaa cggaaacaua     2400

uuaaaggaau accccacgga caaccaggcc cuacagaaug aacgacucuu ccuauacuac     2460

cuucaaaacg gcagagauau guacacagga aaggcauuag acaucgacaa ccuuagccag     2520

uacgacauag aucacauuau accgcaagcu uucauaaaag augacucaau agauaacaga     2580

guccuugugu cgucagcaaa aaacagggga aaauccgacg auguaccuag ucucgaaauc     2640

guaaaagacu guaaagucuu uuggaagaaa cuucuagacg cuaaacuuau gagccaaaga     2700

aaauaugaca aucucacuaa agcagaaagg ggaggccuaa caucagauga uaaagcaaga     2760

uucauccaac gacaacuagu ugaaacccga caaauuacca aacaugucgc ucgaauucuu     2820

gaugaaagau ucaauaacga auuagauucu aaaggucguc ggauaaggaa aguaaagaua     2880

guaacacuga aaaguaacuu aguaucgaau uucagaaaag aauucggauu cuauaaaauu     2940

agagaaguaa auaacuacca ucacgcacac gacgcauacc uaaacgcggu ugucgcaaaa     3000

gcaauacuca caaaauaccc gcaacuagaa ccagaguucg uauacggaga cuacccgaaa     3060

uacaacaguu acaagacaag gaagucagca acagaaaaau uauuuuuuua cuccaauaua     3120

augaacuuuu ucaaaacaaa aguuacuuug gcagauggaa caguaguagu uaaagacgac     3180

aucgaaguca auaaugacac aggagaaauc guaugggaca aaaaaaaaca cuucgcaacu     3240

guucgcaaag uauuauccua uccccaagua aauauuguaa aaaaaacaga aauacaaacc     3300

ggagguuuca gcaaggaauc aauacuagcu caugguaauu ccgacaagcu uauaccacgc     3360

aaaacgaagg auauauaccu agacccgaag aaauauggag gcuuugacag cccaauugua     3420

gccuauucag uacuaguagu ggcagauaua aaaaaaggaa aagcacaaaa acuaaaaaca     3480

guaaccgaau uauuaggcau aacaauaaug gaaaggagcc gcuuugagaa aaauccuuca     3540

gcauuucuug aaucaaaagg auaucuuaac auuagagaug acaaacucau gauccuacca     3600

aaauacucac uuuuugaacu agaaaacgga agacggagau uacuagcaag ugcuggugaa     3660

uuacaaaaag gaaacgaacu agcccuacca acacaauuca ugaaauuucu auaucuugcg     3720

ucucgauaua augaacuuaa agguaaacca gaagaaauug aacaaaaaca agaguucguu     3780

guccaacaug ucucuuacuu ugaugacauc cuacaaauaa uuaacgacuu cucaaacagg     3840

guaauccucg cugaugccaa ucuagaaaaa auaaauaaau uguaccagga caauaaagaa     3900

aauauaagug uugacgaauu agcaaacaau auaauuaauc ucuucaccuu cacaucucuu     3960

ggugccccgg cagcauuuaa auucuucgac aaaauaguag auagaaaaag auacacauca     4020

acuaaagaag uacuuaacuc aacacugaua caccaaucca uaacagguuu auaugagacc     4080

agaauagacu uaggaaaacu aggcgaagac uga                                  4113


<210>  5462
<211>  4113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5462
augaacaaac ccuauagcau cggauuagac auagggacaa auuccguagg auggucaaua       60

auaacagaug acuacaaagu uccagcuaag aaaaugcgug ugcuuggaaa uacagacaaa      120

gaauauauaa agaaaaaucu uauuggagca cuuuuauucg acggaggaaa cacagcagca      180

gauagaagac uuaaacguac agccagaaga agguauacaa gaagaagaaa uagaauauua      240

uacuuacagg aaauauucgc ugaagaaaug agcaaaguag augacucauu uuuccaucga      300

cucgaagaca guuucuuggu cgaagaagac aaacgaggaa guaaauaucc aauuuucgca      360

acaaugcaag aggaaaaaua cuaccaugaa aaauucccca cuaucuauca ucuaagaaaa      420

gagcucgcug acaagaaaga aaaagcggac uugcgccuaa uauaucuagc acuagcgcau      480

aucauaaaau ucagaggaca uuucuugauc gaagacgaca gauucgaugu cagaaacacc      540

gacauucaaa aacaauacca agcauuucuu gaaaucuucg acacuacauu ugaaaauaac      600

gaccuauuau cacaaaacgu agacguagaa gcuauacuaa cagacaaaau cagcaaaagc      660

gcaaagaaag accgaauauu agcaagauac ccaaaccaga aaagcacagg cauauuugcu      720

gaguuccuca aacucaucgu cggcaaucaa gccgacuuua agaaacauuu caaucuagaa      780

gauaaaacuc ccuuacaauu cgccaaagau ucauacgaug aagaucuaga aaauuuacua      840

ggucaaauug gagaugaauu ugcagauuua uucagugcgg cuaagaaacu auacgauuca      900

guuuuacuuu caggcauucu aaccguaacc gacuuaagua caaaagcucc ccuaucagcu      960

aguaugauac aacauuacga ugaacauaga gaagauuuga aacaguuaaa acaauucgua     1020

aaagcauccu ugcccgaaaa auaccaagaa auauuugcag auucaucuaa agauggauau     1080

gcuggcuaca ucgaagguaa aacaaaccaa gaagcauucu acaaauaccu gucuaaacua     1140

cuuaccaaac aggaaggcuc ugaauaccuc cuugagaaaa ucaaaaauga agauuuccua     1200

aggaaacaaa ggacuuucga uaacgguucu auaccccauc aaguacaucu uacagaacua     1260

cgagccauaa uacguagaca aagcgaauau uacccauuuu uaaaagaaaa cuuagauaga     1320

aucgagaaaa uacuuacauu ccgaauaccc uauuacgugg gaccacuagc uagagaaaaa     1380

ucagauuuug cauggaugac aagaaaaaca gaugauucaa uuagaccaug gaacuucgaa     1440

gaucucguag acaaagaaaa aucggccgaa gcauucaucc acagaaugac aaacaaugau     1500

cuguacuuac ccgaagaaaa aguauuaccg aaacauagcc uaauauacga aaaauuuaca     1560

guuuauaaug aauuaacaaa aguacgauuu cuagccgaag gauuuaaaga uuuucaauuu     1620

cuuaauagga aacagaaaga aacuauauuc aauucccuuu ucaaagagaa aagaaaagua     1680

acugaaaaag acauaaucuc auuccuuaau aaagucgacg gauaugaagg aaucgccauc     1740

aaaggcauug aaaaacaauu uaaugccucg cuaucuacau aucaugaucu caagaaaaua     1800

cucgggaaag auuucuuaga caauacugac aacgaauuaa uauuagaaga cauaguacaa     1860

acgcuaacuu uguuugaaga cagagaaaug auaagaaaga gacuugacau auauaaggau     1920

uucuuuacgg aaucccaacu gaagaaauua uaucgccgac acuacaccgg cugggguaga     1980

uuaucugcaa aauuaauaaa ugguauucgu aauaaagaaa accagaaaac uaucuuagau     2040

uauuuaaucg acgacgguuc agccaacaga aauuuuaugc aacuaauuaa agacgcaggc     2100

cuuucguuua aaccuauaau agauaaagca agaacaggcu cacauagcga caaucugaaa     2160

gaagucguag gugaauuagc gggcucccca gcaauaaaga aagggauacu ucaaucauua     2220

aaaaucguug acgaauuagu aaaagucaug gguuacgaac cagaacaaau ugucguagaa     2280

auggcaaggg aaaaucaaac uacugcuaaa gggcuaagua gaucaaggca acgcuuaaca     2340

acucuaagag aaagucuagc caauuuaaaa agcaauauac uagaagaaaa gaaaccuaaa     2400

uacguaaaag aucaagucga aaaccaccac cuuucagaug auagacuauu ccuauacuac     2460

uuacaaaaug guaaagacau guacaccgac gacgaauuag acauagauaa ccucucacaa     2520

uaugauauag aucauauaau accacaagcg uucauuaaag augauucaau agauaauagg     2580

guauuaguau cgucggcuaa aaaucgugga aaaagugaug augucccaag ugucgaaauc     2640

guuaaagauu gcaaaguauu cuggaagaaa cuguuagaug caaaauuaau gucacaaaga     2700

aaauacgaua accucaccaa agcagaaaga gggggauuaa ccuccgacga caaagccaga     2760

uucauacaac gacaacucgu ugaaacacga caaaucacca aacaugucgc uagaauacua     2820

gaugaacgau uuaauaauga acucgacuca aaggguagaa ggauaagaaa gguaaaaaua     2880

gucaccuuaa aaucuaaucu cguaucaaac uuccgaaaag aguucggauu uuacaaaauu     2940

cgagaaguca auaauuacca ccaugcacau gaugcauacu uaaaugcagu cgucgccaaa     3000

gccaucuuaa ccaaguaccc ucaacuggaa ccagaauuug ucuacggaga cuaucccaaa     3060

uacaauaguu auaaaacccg aaaaagugcg accgaaaaau uauucuuuua cucaaacauc     3120

augaacuucu uuaaaacaaa ggucacucua gcggacggua cagucguggu caaagacgac     3180

aucgaaguca acaaugacac cggagaaaua gugugggaua aaaagaaaca uuuugcaacu     3240

guacguaaag uguuaaguua cccacaaguc aacauuguaa agaaaacaga aauacaaacc     3300

ggggguuuca guaaagaauc aaucuuagcg cauggaaauu ccgauaaacu gauaccacgc     3360

aaaacuaaag auauauaccu ugauccaaag aaauacggag gcuucgacag uccaauagug     3420

gcguauagug uauuaguagu agcagauaua aagaaaggaa aagcucaaaa acuuaaaaca     3480

guaacugaac uacuagguau aacaauaaug gaacguucac gauucgagaa aaauccauca     3540

gcauuccucg aaagcaaagg cuaccuaaac auucgugaug auaaacuuau gauccuacca     3600

aaauauagcu uauuugaacu agaaaacggc cgacgcagac uacucgcauc cgcgggugag     3660

cuucaaaaag guaacgaacu agcauuacca acccaauuua ugaaauucuu auaccuagca     3720

ucuagauaca acgaacuuaa aggcaaaccc gaagaaaucg aacaaaaaca agaauuugua     3780

guucaacaug ucaguuauuu cgaugauaua uuacaaauaa uaaacgacuu uagcaauaga     3840

guaaucuuag cagaugcgaa ccucgagaaa auaaacaaac uauaccagga caauaaagaa     3900

aacauuccag uagacgaacu agcgaauaac auaauuaacc ucuucacuuu uacuucucuu     3960

ggugcuccag cagcguuuaa auucuucgac aaaauaguag auaggaaaag guacacaagu     4020

acaaaagaag uauuaaacag cacacuuauc caucaaagca ucacagggcu uuaugaaaca     4080

cgaauagacu uaggaaaacu aggagaggac uga                                  4113


<210>  5463
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5463
augaaaguaa auaauuauca uauaggauua gauauuggca caucauccau cgguugggua       60

gcaauaggag aagacggaaa gccacuccgc aucaaaggaa aaacugcuau aggagcacgc      120

cuauuccaag aagguaaucc ugccgcagau cguagaaugu ucaggaccac acgacguagg      180

uuaagcagaa gaaaauggcg ucuaaaauua cuagaagaga uauucgaucc uuauauaaca      240

ccagucgaca gcaccuuuuu ugcuagacug aaacagagca accuaagucc aaaagauucc      300

aggaaagaau ucaaaggcag caugcucuuu ccagaccuca cagacaugca auaccacaaa      360

gacuauccga caauuuauca ucuacgacac gcauuaauga cucaagacga aaaauucgau      420

aucagaaugg uauacuuagc cauacaucau auaguuaaau acagaggcaa uuuuuuaaau      480

ucuacuccag uggacucuuu uaaggcauca aaagucaacu ucguagacca auuuaaaaaa      540

uuaaacgagu uauacacagc gaucaaccca gaagaaaguu uccagauaaa ucucgcaaac      600

agcgaagaua uuggacacca auuucucgac ccaagcauac gaaaauucga caaaaaaaaa      660

caaauuccaa aaauaguacc gguaucggua gaugacaaag ccacugacaa gauaaaugga      720

aagauugcga gcgaaauaau aaacgcaaua cucggguaca aaucaaaacu ggauguagua      780

guucaaugua caccgguaga uucaaaauca ugggccuuga aauucgacga agaagacauu      840

gacgcgaaac uacaaaaaau acucccagag auggaugaaa aucagcaauc aauaauagca      900

auauuacaaa acuuauacag ccaaguaacg uuaaaccaaa uaguaccaaa uggcaugagc      960

cuaucagaaa guaugauaga aaaauauaac gaucaccacg aucauuuaaa acuguauaaa     1020

aaaauaauag aucaacuagc cgacccaaaa aaaaaagcag ccuugaaaaa agccuacagu     1080

caauauguag gugaugaugg aaaaguaauc gaacaagcug acuuuuggag cagcguuaaa     1140

aaaaaucuag augacucuga uuuaucgaaa cagaucaugg auuugauuga cgcagaaaaa     1200

uucaugccaa aacaaagaac aagccaaaau ggcguaauac cacaucaacu acaccaacgc     1260

gaauuagacg aaauuauaga acaccaaagc aaguauuacc cgugguuagc agaaauaaau     1320

ccaaacaaac augaccuaca uuuagcaaaa uacaaaauag aagaauuagu agccuuccga     1380

gugccuuacu auguaggucc aaugauaacu ccggaugauc aagcaaaauc ugcggaaacu     1440

guuuucuccu ggauggaaag aaaagggaaa gaagccggac aaauuacacc auggaacuuu     1500

gaugaaaagg uagacagaaa ugcaucagcg aauagauuua uuaaaagaau gacaacaaaa     1560

gauacuuauc ucauagguga agauguacua ccagacgaaa gcuuguuaua ugaaaaauuu     1620

aaagucuuaa acgaacugaa uaugguccgu guaaauggaa aacugcuuaa aguugcggac     1680

aaacaagcaa uauuucagga uuuauucgaa aacuauaaac auauaucggu aaaaaagcua     1740

caaaacuaua uaaaaucaaa aaccggucua ccaagugauc ccgaaauauc aggacucuca     1800

gaucccgaau acuuuaacaa cucuuuggga acauacaaug auuucaaaaa acuuuucgga     1860

aauaaaguag augaacccga ccuccaggac gacuuugaaa aaauugucga auggucaacu     1920

guguucgaag acaaacgaau ucuucgagaa aaauuaaacg aaauuaccug gcuuagugac     1980

caacaaaaag auguauuaga aaguucaaga uaccaagggu ggggacgauu gagcaaaaaa     2040

cuucucaccg gaaucguaaa cgaccaagga gaaagaauaa uagacgaacu auggaacacg     2100

aacaaaaauu ucaugcaaau acaaagugau aacgacuuug caaaacguau acaugaagca     2160

aacgccgauc aaaugaaagc cguagacgua gaagaugucc uagcagacgc auauacaagc     2220

ccacaaaaua aaaaagccau aagacaggua guuaaagugg uagacgauau acaaaaagca     2280

augggcggag uagcccccaa auacauaucu auagaauuua cccguuccga agaucggaac     2340

cccagacgua caauaaguag acaaagacaa cuagaaaaca cgcucaaaga uacggcuaaa     2400

ucucuagcaa aaucaauaaa uccugaacua uugagugaac uugauaacgc ugcuaaaucc     2460

aaaaaagguu uaacggaccg acuauauuua uacuuuacac aauuagguaa agauauuuac     2520

acaggaaaac caauaaacau cgaugaaaua agcacauacg acauagauca cauacuuccc     2580

caagcguucg uaaaagacga cuccuuaaau aacagaguac uaguuaguaa agcaauaaau     2640

aacgguaaau cugauaaugu acccguucaa cuuuuuggug caaaaauggg acauuucugg     2700

aaacaacuag ccgaagccgg guuaauaucc aaaagaaaac uaaaaaaucu acaaacugac     2760

ccagacacca ucucaaaaua ugccaugcac ggauucauua ggagacaacu uguugaaaca     2820

agccaaguaa uaaaacucgu ugcaaacaua cuaggagaca aauaccguaa ugacaacacu     2880

aaaauuauag aaauaacugc gagaaugaau caucaaaugc gugaugaauu cggguuuauu     2940

aaaaacaggg aaauaaacga cuaccaccac gcauuugacg ccuaucuaac ggcauuuuug     3000

gggagauacc uauaucacag auauaucaaa cuacgaccau auuucgucua cggagauuuu     3060

aaaaaauuua aagaagacaa aguuacaaug cgaaauuuua auuuucuaca cgaccuaacu     3120

gaugacacgc aagaaaaaau agccgacgcc gaaacgggag aaguaauaug ggacagagaa     3180

aauagcaucc aacaacuaaa ggauguauac cauuauaaau uuaugcuaau cagucaugag     3240

gucuauaccc uccguggggc cauguucaau caaacaguuu acccagcuuc agacgcaggu     3300

aaaagaaaac uaauucccau uaaagcugau agaccuauaa acguauacgg gggauauucc     3360

ggcuccgcug augcuuacau ggcaauaguu aggauacaca auaaaaaagg ggauaaauau     3420

agaguaguag gugucccaau gcgagccaga gacaggcuag acgcugccaa aaaaguuuca     3480

gaugcugacu gcgacagagc auugaaggau guacuuacgc cacaacuaac aaaaacaaaa     3540

aaauccagaa aaacgggaga aaucacgcag gucguugaag auuuugagau agucuuggga     3600

aaagucaugu auaggcaauu aaugauugac ggugacaaaa aauucaugcu uggaucauca     3660

acguaccaau acaacgccaa acaacuugua uugucagauc aaucggucaa aacacuagcu     3720

ucaaaaggaa gacuagaucc gcuccaagaa ucaauggauu auaacaaugu uuacacagaa     3780

auauuagaua aaguuaacca auauuucucu cuauacgaca ugaacaaguu uagacauaaa     3840

cuaaacuugg gauucucgaa auucauuucg uuucccaauc auaacguauu ugacgguaac     3900

acaaaagcau caucaggaaa aagagaaauc cuagaagaag uauuaaaugg acuccacgca     3960

aacccaaccu ucggaaaucu uaaagacaua gguaucacca caccauucgg ucaauuacaa     4020

caacccaacg guauccuucu uagcgaugaa gcaaaaauac guuaccaauc accaacaggu     4080

cuauuugagc guacuguauc cuuaaaagau cuauga                               4116


<210>  5464
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5464
auggacaaaa aguacaguau aggccuagau auaggaacaa auaguguagg augggccgua       60

auaacagacg acuauaaagu gccaucaaag aaauucaaag uucuaggcaa uaccgauaga      120

cauucuauaa agaaaaaccu aauaggcgcu uuacucuucg auucgggaga aaccgcggaa      180

gcgacacgac uaaaaagaac agcccgcagg cgcuauacua gaaggaaaaa ccgaauccga      240

uaucuacaag aaauuuucuc aucugaaaug agcaaagucg acgacucauu cuuucacaga      300

uuagaagaau cauuuuuagu agaagaagau aagaaacaug aaagacaucc uauauuugga      360

aacauuguag acgaagucgc cuaucaugaa aaauacccaa caauauauca ucuccguaag      420

aaauuagccg auucaacaga uaaagcagac cuacgacuaa uauaucuugc uuuagcacau      480

augauaaaau ucagaggaca uuuccuaauu gagggcgacc ugaaccccga uaacagcgau      540

auggauaaau uauucaucca acuuguucaa acauacaauc aacuguucga agaaaaucca      600

auaaacgcau cuagaguuga cgcaaaagcc auccuuucag caagauuauc gaaaagucga      660

cgacuugaaa aucuuaucgc ucaacuaccc ggagagaaaa gaaauggauu auuugggaau      720

cuaauagcac uauccuuagg ucuaacacca aacuuuaaaa guaacuucga uuuagcugaa      780

gacgcaaaau uacaacuuuc aaaagauaca uaugaugacg accuggacaa ccuauuagcu      840

cagaucggug aucaauaugc agaucuauuu cuugcugcua aaaaccuguc cgacgcaauu      900

uuacuaagug acauacuaag aguaaacucu gaaauaacaa aagcuccccu uuccgccucu      960

augaucaaaa gauacgacga acaccaucaa gaccuaacac uacucaaagc auuaguaaga     1020

caacaacucc cggaaaaaua uaaagagauc uucuuugacc aaucuaaaaa cgguuaugcu     1080

gguuauauug acgguggagc gucacaagaa gaguucuaca aauucauaaa accaauauua     1140

gagaaaaugg auggaacuga agaauuauua gcuaaacuca aucgugaaga ccuacuuaga     1200

aagcagagaa cauucgacaa cgggucaaua ccacaucaaa uacaucuagg cgaauuacau     1260

gccauacuaa gacggcagga agauuuuuau ccauuccuaa aagacaacag agagaaaaua     1320

gaaaagauuu uaacauucag aauacccuau uauguaggac cacucgcaag aggcaacucg     1380

agauucgcau ggaugacacg uaaaagugaa gaaaccauca caccauggaa cuuugaagaa     1440

guaguagaca aaggagccag ugcccagucu uucauugaaa ggaugacaaa uuucgacaaa     1500

aaccuaccaa acgaaaaagu acuaccuaaa cauucgcuau uauaugaaua uuuuaccgua     1560

uacaacgaau uaacuaaagu aaaauaugug acagaaggua ugcguaaacc agaauuuuua     1620

ucggguaagc aaaaagaagc gauuguagac cuccuauuua aaacaaauag aaaaguaaca     1680

guaaaacaac uuaaagaaga uuacuuuaag aaaauagaau gcuucgacag uguagaaaua     1740

uccggaguag aagaccguuu uaacgcaagu cuagguacau accaugacuu auuaaaaauu     1800

auaaaagaca aagacuuucu ugacaaugaa gaaaaugaag acauccuuga agauaucgua     1860

uuaacgcuua cgcuauuuga agauaaagaa augauagaag aacgccuaaa gaaauaugca     1920

aauuuauucg acgacaaagu gaugaaacaa cuaaaacgua gacauuauac cggcugggga     1980

agacuuucac gaaaauugau uaauggaaua agggacaaac aguccggaaa aacaaucuua     2040

gacuuucuaa agagugacgg cuucgccaac cgaaacuuua ugcaauuaau aaacgacgau     2100

ucuuugaccu uuaaagaggc aauacagaaa gcacaaguau caggucaagg ccacucauua     2160

caugaacaaa uagcaaacuu agccggcagc ccugccauaa agaaaggaau acuacaaagc     2220

guuaaaguag uggaugagcu agucaaaguc augggacaca aacccgaaaa cauaguaauc     2280

gagauggcua gagaaaauca aacuacccag aaaggacaga aaaauucacg ugagcggaug     2340

aaaagaauag aagaaggaau aaaagaacuc ggaucacaaa uuuuaaagga gcacccugua     2400

gaaaacacuc aauuacaaaa cgaaaaauua uaucuguacu aucuacaaaa uggacgagac     2460

auguauguug accaagaauu agacauaaau cgacuaagcg acuaugacgu agaccauauu     2520

guaccacaau cauucauaaa agacgauuca auagauaaua aaguacucac acgaucagac     2580

aaaaacagag ggaagucaga caauguaccc ucagaagaag uagucaaaaa gaugaaaaau     2640

uauuggagac aacuguuaaa cgccaaauua auaacacaaa gaaaauuuga caaccuaacu     2700

aaagcagaaa gaggaggacu aagcgaacuc gauaaagcag guuuuauaaa acgccagcua     2760

guagaaacac gacaaauaac aaaacauguu gcucaaauuu uggacucacg caugaacacc     2820

aaguaugacg aaaacgauaa acucaucaga gaaguuaaag uaaucacucu aaagucuaag     2880

cuaguaucag auuuucguaa agacuuccaa uuuuauaaag uccgugaaau aaacaauuau     2940

caucacgccc acgacgcaua cuuaaaugca guuguuggua ccgccuuaau uaagaaauac     3000

ccaaaacucg aaagcgaauu uguauacggc gacuauaaag ucuacgaugu uagaaaaaug     3060

auagcuaaaa gcgaacaaga gaucggcaaa gcgacagcaa aaagauucuu uuacucaaau     3120

aucaugaauu ucuuuaaaac cgaaauaacg cuugcaaaug gagagauaag aaaacgucca     3180

cuuauagaaa caaacgaaga aacuggggaa aucguguggg acaaaggaag agacuuugcu     3240

acaguuagaa aaguucuaag caugccacaa guuaauauug ugaagaaaac ugaaguacaa     3300

accggagcuu uaacaaacga aagcauauac gcaagaggau cauuugacaa acuaauaucc     3360

agaaaacaca gauucgaguc aucuaaauau gguggauucg gaagcccaac agucacauac     3420

ucaguacugg uaguagcaaa aagcaaagua caggauggca aaguuaagaa aauuaaaaca     3480

gguaaagaac uaaucggaau cacguuauug gauaaauugg uauuugagaa aaauccucuu     3540

aaauucauug aagauaaagg auacggcaau guacaaauag auaaauguau aaaacuccca     3600

aaauauagcu uauucgaauu ugaaaaugga acuagaagaa ugcuagcaag uguaauggcu     3660

aacaacaaua gcagaggaga uuuacaaaaa gcgaaugaaa uguuccuacc agcaaaacuc     3720

guaacacuac ucuaccaugc acauaaaaua gaauccagca aagaauuaga acaugaagca     3780

uauauauuag accacuauaa cgaccucuac caacuuuuaa guuacauaga aagguucgca     3840

agucuguacg uagacguaga gaaaaacauu aguaaaguua aagaacuauu cagcaauaua     3900

gaaaguuaca gcaucucaga aaucugcucu agcguaauua aucuacuaac gcuaacagcc     3960

aguggugcgc cugcugauuu caaauuccua ggaacaacca uuccuagaaa aagauaugga     4020

aguccacaaa guauuuuauc aucuacccua auucaucaau caauaacagg acuauaugaa     4080

acgcgaauag aucuaagcca acuaggaggg gacuga                               4116


<210>  5465
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5465
auggacaaga aauacagcau aggucuagac auuggaacga acucuguagg augggcagug       60

auuacagacg acuauaaagu uccaucuaag aaauuuaaag ugcuaggcaa cacugaucga      120

cauaguauaa agaaaaacuu aaucggcgcc uugcuauucg acucuggcga aaccgcugaa      180

gcaacaaggc uaaaaagaac agcaagaaga agguacacaa gaaggaaaaa cagaaucagg      240

uaucuacaag aaaucuuuuc cagcgagaug ucaaaaguag acgauaguuu cuuccaucgg      300

cuugaagaau cuuuccuugu agaagaagac aagaaacaug aaagacaucc uauuuuugga      360

aauauugugg acgaaguagc auaccacgaa aaauauccua ccauuuacca uuuacgaaag      420

aaauuggcag auaguacaga uaaagcagau uuaagauuaa uauaucuagc uuuagcccac      480

augaucaaau uuagaggaca cuuucuaaua gagggagacc uaaaucccga caauagcgac      540

auggacaaac uauucauaca auuaguccaa acauacaacc aauuauucga agaaaaccca      600

auaaaugcgu cacguguaga ugcuaaagcc auccuuagug caagauuauc aaaaagcaga      660

agauuagaaa accuaauugc acaacugcca ggugagaaaa gaaacggacu uuuuggaaac      720

cuaaucgcac ugucuuuagg gcucacacca aauuucaaau caaauuucga ccuggcagaa      780

gacgccaaau uacaacuauc uaaagauaca uacgaugacg accuugauaa ccuacuagca      840

caaauaggug aucaauaugc ggacuuauuu uuagcugcga aaaauuuauc agaugcaaua      900

cuguuaagug acauuuuaag aguaaacucu gaaauaacaa aagcaccauu aagcgcauca      960

augauaaaaa gauacgacga acaucaccag gaccuaacac uacuaaaagc ccuaguaagg     1020

caacaacuac cagaaaaaua uaaagaaaua uuuuucgacc aaaguaaaaa uggauacgcc     1080

ggauauauug auggaggagc aucccaagaa gaauuuuaca aauuuauaaa acccauuuua     1140

gagaaaaugg acgguaccga agaacuauua gcaaaauuaa auagagaaga uuugcuucga     1200

aaacaaagaa cuuuugacaa cggcucuaua ccgcaucaaa uacaucuagg ugaauuacau     1260

gcuauauuaa gacgccaaga agauuucuau ccauuucuua aagauaacag agagaaaaua     1320

gagaaaauac ugacauuuag aauaccuuac uauguaggcc cacuugcuag ggguaauuca     1380

cgauucgcau ggaugacaag aaaauccgaa gaaacgauaa caccauggaa cuuugaagaa     1440

gucguugaca aaggcgccuc ugcacaaucc uucaucgaaa gaaugacaaa uuuugauaaa     1500

aaccuaccaa acgagaaagu guuacccaag cacucucucu uauaugaaua cuuuaccguc     1560

uacaaugaac ucacaaaggu aaaauauguc acagaaggaa ugcgaaaacc agaauuucua     1620

ucagggaaac aaaaggaagc uaucgucgau cucuuauuca aaacaaacag aaaagucaca     1680

gugaaacagu uaaaggaaga cuauuuuaag aaaauagaau guuuugacag cgucgaaaua     1740

aguggagucg aagaucgauu caaugcauca cuuggcacau aucacgaucu uuuaaaaauc     1800

auaaaagaca aagauuuucu cgauaacgaa gaaaaugaag auauucuuga ggacauugua     1860

cuaacauuaa cacuauucga agacaaagaa augaucgaag aacgacuaaa aaccuacgcu     1920

caucuauucg augauaaggu caugaaacaa cuaaaaagac gacauuacac aggaugggga     1980

aggcuaucaa gaaaauuaau uaacggaaua agagacaaac aaucaggaaa aacaauauua     2040

gacuuucuca aaucagaugg auuugcuaau cguaauuuca uacaauuaau acacgacgau     2100

ucacucacgu ucaaagaagc uauacaaaaa gcucaagucu ccggucaagg acacagucua     2160

cacgaacaaa uagcaaaccu agcaggaagu ccugccauaa agaaaggaau ccuacaauca     2220

gugaaaguag uugaugagcu cguaaaggua augggacaua aaccagaaaa cauagucauc     2280

gagauggcaa gagaaaacca aacaacacaa aaaggacaga aaaauucaag agaaagaaug     2340

aaaagaauag aagagggcau aaaagaacua gggucgcaaa uacuaaagga acacccugug     2400

gaaaacacac aacuacaaaa cgaaaaacua uacuuauacu accuucaaaa cggcagagau     2460

auguauguug aucaagaacu ugauauaaac cgccuaagug auuaugacgu ugaucauaua     2520

gucccacaau ccuucaucaa agacgauucc auagacaaua aaguacuuac aagaagcgac     2580

aaaaauagag guaaaucaga cgacgugcca ucugaagaag uugugaagaa aaugaaaaac     2640

uacuggcgac aauuguuaaa cgcgaaacuc aucacacaaa gaaaauucga caaucuuacc     2700

aaagcagaaa gagggggauu aucggaacua gacaaagcag gcuucaucaa aagacagcuu     2760

guagaaacca gacaaaucac uaagcauguu gcucaaaucu uggacucgag gaugaauacu     2820

aaauacgacg agaacgauaa acugauuaga gaaguaaaag uaauaacucu aaagucuaaa     2880

uuagugagug acuucagaaa agacuuucaa uucuauaaag uaagagaaau aaauaauuac     2940

caucacgcuc augaugcaua uuuaaacgca guaguaggga cggcauuaau aaagaaauau     3000

cccaaauuag aaucagaguu cgucuauggu gauuauaaag uauaugacgu caggaaaaug     3060

auugcaaaaa gcgaacaaga aauugggaaa gcaacugcaa aacguuucuu cuacucaaac     3120

auaaugaauu ucuuuaaaac cgaaaucacu cucgcaaaug gagaaauccg aaaacgacca     3180

cuaauugaga cuaaugaaga aacaggagaa auugugugga acaagggacg cgacuucgcc     3240

accguaagga aaguacuaag uaugccccaa guaaacaucg uaaagaaaac agaaguucaa     3300

acaggagcuu uaacuaacga aucgauauau gcgcguggau cauuugauaa auuaauuuca     3360

agaaaacauc gauuugaauc gagcaaauac ggaggauuug gaagcccaac agucacauau     3420

ucuguauuag uuguagccaa aucaaaaguc caagauggga aaguaaagaa aaucaaaacc     3480

ggaaaagaau uaaucgggau uacauuacua gauaaacuug uauucgagaa aaacccacua     3540

aaauuuauag aagauaaagg auauggaaac guccaaauag auaaaugcau aaaacuacca     3600

aaauauaguu uauuugaguu cgaaaacggg accagacgua ugcucgccuc gguaauggcu     3660

aauaacaauu caagaggcga cuuacagaaa gccaaugaaa uguucuuacc agcgaaauua     3720

gucacacuac uguaccacgc ccacaaaauc gaauccucaa aagaacugga acacgaggcu     3780

uacauccuug accacuacaa cgaccuguau caacuacuau cuuauaucga acgauucgca     3840

ucauuauacg uagacgucga aaagaacaua agcaaaguua aggaauuguu cucuaauaua     3900

gaaaguuacu cuauaucaga aaucuguucg agcguuauaa acuuacugac auuaacggca     3960

ucaggagcac ccgcagacuu caaauucuua ggaaccacga uaccacgaaa acgauacgga     4020

ucaccgcaau caauuuuguc aucaacacua auacaccaau caauaacugg ccucuaugaa     4080

acuaggauag auuuauccca acuaggagga gacuga                               4116


<210>  5466
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5466
augaacaaua acaacuauag uauaggccua gacauaggca caaacagugu agguugggca       60

guaauaacgg augacuauaa aguaccaagu aaaaaaaugc gcguacuggg aaauacagac      120

aaacguuuca ucaagaagaa ucuaauaggc gcucuccuau ucgaugaagg aaccacagca      180

gaagacagaa gauuaaaaag aacagcacgc cgacgauaua cuagaaggaa aaacagacua      240

agauaccuuc aagaaaucuu cgcugaagaa augucuaaag ucgauucuag uuucuuucac      300

agacuugaug acaguuuucu aguuccagag gauaagucag guucuaaaua uccuaucuuu      360

gcaaccuugg cugaagaaaa agaauaccac aaaaaauucc cgacuauaua ccaucuaaga      420

aaacaccucg ccgauucaaa agaaaaggca gaucuaagac uaauauaucu agcgcuagcu      480

cacauuacca aguaccgcgg ccauuucuug uacgaagaag ccuuugauau uaaaaacaac      540

gacauccaga aaauauuuaa cgaauucauc aacauauaug auaauacauu cgaaggcucu      600

ucacuuucug ggcaaaacgc ccaaguagaa gcaauuuuca ccggcaaaau aagcaaaucc      660

guaaaacgag aacacguacu aaaauuguuu ccggaugaaa aaucgaccgg acuauuuuca      720

gaguucuuaa aacugauagu aggaaaccaa gccgauuuca agaaacauuu cgacuuggaa      780

gaaaaagccu cacuacaauu uagcaaagau acguaugaug aagaucugga aaaucuacuc      840

ggacaaauag gcgacgacuu ugccgaccua uuccugguag ccaaaaaauu auaugacgca      900

auccuccuau ccggaaucuu aacgguaacc gauccaucca caaaagcucc cuuaagugcc      960

ucaaugauag aacgauauga aaaccaucaa gaagacuuag ccgcccuuaa acaauucauc     1020

aaaaacaacu uaccagaaaa auacgcggag guuuuuagcg accaguccaa ggauggcuau     1080

gccgguuaua uagacggcaa gacuacucaa gaagccuuuu acaaauauau uaaaaauuua     1140

uuauccaaau ucgaaggcgc agauuacuuu cuagacaaaa uagaaaggga agacuuccua     1200

cgaaaacaac ggaccuuuga caaugguagu auaccccacc aaauacaccu ccaagaaaug     1260

aacgcgauac uaaggcgaca aggagaacac uauccccuac uaaaagaaaa uaaagaaaaa     1320

auagagaaaa uauugacauu ccgaauacca uauuacguag gaccacuagc aagaggaaac     1380

agggauuucg cuugguugac aagaaacagc gaccaagcua ucaggcccug gaauuucgaa     1440

gaaauugucg auaaagccag uucagcagaa ucuuuuauaa auaaaaugac aaauuaugac     1500

cuauaccuac cugaagaaaa aguauuaccu aaacauaguc ucuuauauga gacauucgcu     1560

guauauaacg aacuaacuaa aguuaaauuu auagcggaag gccuaagaga uuaccaauuc     1620

uuugacucag gccaaaaaaa acaaaucgua aaccaauugu ucaaagagaa aagaaaagua     1680

accgaaaaag auauuauaca uuaccuacau aaugucgacg guuacgacgg aauugaacug     1740

aaagggauag aaaaacaauu caacgcaagu cuaucaaccu aucacgaucu auuaaaaaua     1800

auaaaggaca aagaauucau ggauaaccau aaaaaccaag aaaucuuaga aaauauagua     1860

cacacccuaa caauauucga agaccgagaa augauuaaac aaaggcucgc acaauacgau     1920

uccauauucg acgaaaaagu aauaaaggcg cuuacaagac gccauuauac cggaugggga     1980

aaacucagcg caaaauugau caauggaauu ugugauaaaa aaacaggcaa aaccauuuua     2040

gacuacuuaa uagacgacgg uuauaacaac cgaaauuuua ugcaauuaau aaaugaugau     2100

ggcuuaucau ucaaagaaau cauacaaaaa gcccaaguag uuggaaaaac agaugacuua     2160

acucaaguug uuagagagcu aucgggaagu ccagccauaa aaaaagggau cuuacaaucg     2220

aucaaaauag uagaugaacu agugaaaaua auggguuacg caccagaaag cauagucauu     2280

gagauggcca gagaaaacca aacaacugca aaaggaaaaa aaaacucaca acaaagauau     2340

aaacggauag aagaugcauu aaaaaacuua gccccagguc ucgauucaac gauuucaaaa     2400

gaaaauccaa cagauaacau acaacuacaa aaugaccgac uuuuucuuua cuacuuacaa     2460

aacggaaaag acauguacac cggagaggca cuugacauaa aucaguuauc cucguacgac     2520

auagaccaua uaauaccuca agcauuuauc aaagacgacu cccuagacaa ucguguccuc     2580

acaucuucua aagauaauag aggcaagagc gauaacguac caucacuaga gguaguaaaa     2640

aaaagaaaag cauuuuggca acaacuacug gacuccaaac uaauaucaga aagaaaauuc     2700

aacaaccuaa cuaaagcaga acgcggagga cuagaugaaa gagacaaagu agguuuuauc     2760

aaacgccagc uaguagaaac aaggcaaaua acgaaacacg uugcgagaau acuagaugca     2820

cgauuuaaca cagaaguauc ggaaaaaaau caaaaaauua gaucaguaaa gaucaucacc     2880

uuaaaaucaa auuuagugag uaauuuccga aaagaauuca aauuauauaa aguaagagaa     2940

auaaaugauu aucaucaugc acaugacgca uaucuaaaug caguaguugc caaagcuaua     3000

cuaaaaaaau acccaaaauu agaacccgaa uuuguuuacg gagauuacca aaaauacgac     3060

cuaaaacgau acaucucaaa aucgaaagac ccuaaagaag uugagaaagc aacagaaaaa     3120

uauuuuuuuu acaguaaccu ucuuaacuuc uucaaagaag aagugcacua cgcagacgga     3180

acaaucguaa agagggaaaa uauagaauac ucaaaggaua caggcgaaau agcuuggaau     3240

aaagaaaaag acuucgugac uaucaaaaaa guacuaucau acccucaagu aaacauuguc     3300

aaaaaaagag aaguacaaac agguggauuc ucgaaagaau cgauacuucc aaaaggcaau     3360

uccgauaaac uaauaccgcg aaaaacaaaa gacauacuau gggacaccac aaaauaugga     3420

ggauuugacu cuccaguaau agcuuacagu aucuuacuca uugcagacau ugagaaaggu     3480

aaagcaaaga aacuaaaaac agugaagacu cuaguaggca ucacaauaau ggaaaaagcc     3540

gccuuugaaa aaaaccccau aacguucuua gagaauaaag gauaucauaa uguuagaaaa     3600

gaaaacauac uaugcuuacc aaaauauagc cuauuugagc uagaaaaugg aagaagaaga     3660

cuguuggcua gcgcaaaaga auuacaaaaa ggaaacgaaa uuguacuucc ugucuaucua     3720

acaaccuuau uauaucacuc aaaaaauguu cacaaauugg acgaaccaga acaccuggag     3780

uacauucaaa aacacaggua cgaauucaag gaucuuuuaa auuuagucuc cgaauuuuca     3840

caaaaauacg uccuugcaga agcaaauuua gaaaaaauaa aaucccuaua cgucgauaau     3900

gaacaagccg auaucgaaau ccucgcaaac ucguucauaa aucuacuuac auuuacagcc     3960

uuaggcgcuc cugccgcauu uaaauuuuuc gguaaagacg uagacagaaa aagauacacc     4020

acgguuucag aaauucuaaa cgccacacua auacaccaau caauaacagg cuuauaugaa     4080

acccggauag aucuuucaaa acuaggcgaa gauuga                               4116


<210>  5467
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5467
augacagaga aaaacuacuc uauaggucua gacaucggaa caaacucugu aggaugggca       60

guaauaacgg augacuauaa agugcccgcc aagaaaauga aaguacuagg uaacaccgau      120

aagaaauaca ucaagaaaaa uuuauuagga gcacuacugu ucgacaaugg agaaaccgcu      180

gaagcgacgc gacuaaaaag aacagcacga cgcagguaca cccgaaggaa aaaucgacua      240

agauaucugc aggaaauuuu cgcagaagaa auggcaaaag uagacgagag cuucuucuac      300

cgucuugacg aaucauuccu aacaacagac gacaaagauu ucgaaagaca cccaauauuc      360

ggaaacaaag ccgacgaaau aaaauaccac caagaauuuc caaccauaua ccaccuaaga      420

aaacacuuag cagauagcca ugaaaaagca gaucugcgcc uaauauaucu agccuuagca      480

cacaugauaa aauuucgagg ccacuuuuua auagaaggug aacuaaaugc ugaaaacacg      540

gacguacaaa aauuauuuga agcauucgua gaaguauacg auagaaccuu cgacgauagc      600

aauuuaagcg aaauaacagu agaugcgagc ucuauuuuaa ccgaaaaauu cuccaaaucc      660

agaaggcuug aaaacuuaau uaaacauuau ccuacagaaa agaaaaauac auuauucggg      720

aacuuagucg ccuuagcauu aggccuacaa cccaacuuua aaacauccuu caaauuauca      780

gaagacgcaa aacuacaguu cucuaaagau acauaugaag aagacuuaga agaacucaua      840

ggaaaaauag gagaugaaua ugcagaccua uuuacuucag cuaaaaaccu cuaugacgcu      900

auauuguuaa gcggaauacu aacgguggca gauaauacca ccaaagcacc ccuaagcgcg      960

aguaugauua aaagguauaa cgaacaccaa guagaucuaa agaaacuaaa agaguucauu     1020

aaaaacaacg ccagugauaa auacgacgaa auauucaaug acaaagacaa aaaugguuau     1080

gcaggauaca uagaaaaugg uguaaaacaa gaugaauuuu acaaauaccu aaaaacuacc     1140

cugucaaaaa uagauggcuc ggacuauuuc uuagauaaaa uagaaagaga agauuucuua     1200

aggaaacagc gcacuuucga caacggcagu auaccacauc aaauacaucu ucaagaaaug     1260

cacgcuaucu uacgccgaca aggagaacau uaugccuucc uuaaagaaaa ucaggcaaaa     1320

aucgagaaaa uacuuacauu ucgaauaccc uauuacgugg gaccauuagc ccggaaaaac     1380

agcagauuug caugggcaga auaucauuca gaugagaaaa uaacuccaug gaauuucgau     1440

gaaauaauug auaaagaaaa aucugccgaa aaauucauua caagaaugac acuuaaugau     1500

cuauaucugc cagaagaaaa aguuuuacca aaacacagcc ucgucuauga aacauauaca     1560

guauacaaug aacuuacuaa agucaaauac guuaacgaac aaggcaaauc uaacuuuuuc     1620

gacgcuaaua ugaaacaaga aauauuugaa cauguauuca aagaaaaccg aaaaguaaca     1680

aaagacaaau uucuuaauua ccuaaacaaa gaauuuccug aauaucgaau acaggacuua     1740

auaggauuag acaaagaaaa uaaaucuuuc aacgcuaguc ucgguacgua ucaugacuua     1800

aagaaaauac uugauaaauc auucuuagau gauaaaacaa augaaaccau cauagaagac     1860

auaauucaaa cuuuaacauu auucgaagac agagauauga uccgucaacg ucuacaaaaa     1920

uacucugaua uauucacacc ccaacaacua aagaaauugg aaagaagaca uuacacaggu     1980

uggggaagau uaucauacaa auuaauuaac ggaauacgca acaaagaaaa ugggaaaagu     2040

auuuuagacu aucuaauuga ugacgguuac gcgaacagga acuucaugca auuaauaagu     2100

gacgacacgc uacccuucaa acaaauaaua aaagaugcac aaauuauagg agauauagau     2160

gauguaaccu cagucguacg agaacuccca ggaucccccg caaucaagaa agguauauua     2220

caaaguguca aaaucguuga cgaauuggua aaaguaaugg gccacaaucc agauaacaua     2280

guaauagaaa uggcgcgcga aaaucaaaca acaaacagag gaagaaauca aucacaacaa     2340

agacuuaaga aacuccaaga uagccuaaaa gaauuaggua gcaauauccu aaacgaagaa     2400

aaaccaucau auauagaagg uaaaguagaa aacaaucacu uacaggacga ccgacuauuu     2460

cuauacuaca uacaaaacgg uaaagauaug uacacaggag acgaauuaga caucgaccac     2520

uuaucagacu augacauaga ucauauaauu ccucaagcau uuauaaaaga cgauuccauc     2580

gacaauagag uucucacaag cagugccaaa aauagaggca aauccgacga cguacccucg     2640

cuugauauag uacaugauag aaaagcugac uggauacgac uauacaaaag cggguuaaua     2700

ucaaaacgaa aauuugauaa ccuuacuaaa gccgaaagag guggacuaac agaaaacgac     2760

aaagccggau uuauaaaaag acaauuagua gaaacgagac aaauaaccaa acauguugca     2820

caaauauuag acaguagauu uaauacugaa agagacgaaa augacaaagu aauuagaaau     2880

gucaaaguua ucacauuaaa aucuaaucua gucagucaau ucagaaaaga uuucaaauuu     2940

uacaaaguaa gagaaauaaa ugacuaucac caugcacaug acgcuuauuu aaaugcugua     3000

guagguaccg cccuauuaaa gaaauacccu aaauuagcac ccgaguucgu auacggagaa     3060

uacaagaaau augauauucg aaaauuuauu accagcuccg gugauaaagc aacagcaaaa     3120

uauuucuuuu auucuaaucu aaugaacuuu uucaaaagag uaaucagaua caguaacggu     3180

aaaguaguag uaaggccagu aauagaaugc aguaaagaca caggugaaau agcauggaau     3240

aaacaaacag acuucgaaaa agucagacgg gucuuaagcu auccccaagu aaauaucguc     3300

aagaaagucg aaacccaaac agggggcuuu agcaaagaaa guauauuacc uaaaggcaac     3360

agugacaaac uaauuccaag gaaaacaaag aaauuucggu gggacacacc aaaauacggu     3420

ggauucgauu cgccaaacau cgccuacagu guauucguaa uugccgacgu agaaaaagga     3480

aaagcuaaga aacugaaaac cgucaaagaa uuaguuggua uaaguaucau ggaacgaucu     3540

aguuucgaag aaaauccugu gguauucuua gaaaagaaag gauaccaaaa uguacaagaa     3600

gacaaccuaa uaaaauuacc caaauauagu uuguucgagu ucgaaggagg cagaaggcgu     3660

cuacuugcga gugcaucuga auugcaaaaa gggaacgaag ucguauuauc uagacaucua     3720

guagaacuau uauaucaugc gcaccgaguc aacucauuua auaauagcga acaucuaaaa     3780

uacgucuccg aacacaagaa agaauuugga gaaguauuau caugugucga gaacuucgca     3840

aaaucuuaug ucgacguaga gaaaaacuua gggaaaauuc gcgccguagc agauaaaaua     3900

gauaccuuuu ccauagaaga uauuagcaua ucauuuguaa auuuauuaac acucaccgca     3960

cuuggagccc ccgcugacuu uaacuuucug ggugagaaaa uuccgagaaa gagauacaca     4020

ucaacaaaag agugccuuaa ugcaacucua auacaccaaa gcaucacagg ucuauaugaa     4080

accagaauag aucuaucgaa auuaggugaa gaauga                               4116


<210>  5468
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5468
augaauuuaa gaaauguuga aagcuaucaa gucggacuag acauugguac aggaagugua       60

ggcugggcag uccuagacga caauggugau uuauguagau uuaaaggaaa accgaccugg      120

ggaucacgcg uuuuucccac agcagaaaca gcagcggaag cacgcguaca cagagggcaa      180

agacgaagau augaucgaag gagacaaagg aucgauuuau uacaacguuu cuuugcagac      240

gaaguugcaa aagucgaccc cgaguucuuu auccgacuua aucaggccag acuucaccca      300

gaaaacagag acccugcuca cgcggacuau agauggccac ucuuuaacgg aucaaacuuu      360

acggaaaaag auuauuaucg acaauuuccu accauuuauc accuccgggc acacuuauua      420

aaaucagacg aaccagcaga uauaaggcuu gucuauuuag cacuacauaa uauaguuaaa      480

cacagaggaa auuuucuaca ucaagauaac ccgagcuuau cagcucguaa ugcuaacguu      540

ggagaugcgg uggaagccuu gugcuuggca uuggacgaau ggugcggugc acaugacgua      600

gaaugugcgu gugacgaagc ugcgcuagcg gaagcccuug gagacgcgag cuuacgacca      660

ggggagaaaa gggaacgugc agaagcagcc uuaggggucg aaaaagcaca uaagaaacuc      720

gcuaaagcag uuagcucagc agugguagga uaugcagcug aauuuggaca uguauucuuu      780

gcagaaagug aagguaguaa auuugcguua aguaacgacg aaaaaguaga agccuacgua      840

uguccggacg aaggaagaga uuuguucgau gcgaugcgug ccguccauag uucauacguu      900

cuaaugggaa uauuaagcga agcgcaagga gaauuacuau caguauguaa aguaggagaa      960

uaugaacgau auaaaaggga ccuugaaaca cuuaaaucau uagugcgagc guauggucca     1020

gaaagguaug augaguucuu cugcggacca uuuuaugaag gaacuagaga uuacgacccc     1080

ggaaaagcgc aaggauacac aaaauauaau cucggagcuu caaaauuauc acacgaagac     1140

uuuuuaaaag aagugcaaaa auuacuaaaa gauaucggag cacaagcaga ugaacgauac     1200

gcugacaucg aaagagcaau agaagccggu gcauuccuaa gacgguuaaa aacuucagac     1260

aaugguacua uccccuauca auuacaucug gaagaaaugg augcgauaau agaaaggcaa     1320

gccgcauuuu auccguuucu ggcacaagaa aaagauaaac uaaaaagucu aguaucauuu     1380

cguauaccau auuacguugg accauugaca caaaagaaug cugcuagaga cggaaaagga     1440

gaagcaagau uugcaugguc aagccguaaa ccagggaugg aagacgcgcg aguauacccu     1500

uggaacuggg aagauguaau cgacaaagau caauccgccg aagauuuuau aagaagaaug     1560

acagggacgu gcacauaucu aaaaggagaa ccagugcuug cgcgauguuc acuggauuau     1620

gaauuauuuu gcguucuuaa cgaauuagcg ggagcuagau ggacccaaga cggcgauaaa     1680

agcuauagau ucgaugcugc agauagacua ggaauuguag aagauuuauu caaaaagagg     1740

aagcgaguca cauacgaagc agcagaaaaa uggcuugcuc gaacacaugg acccgaguca     1800

ggaaaauccg caggaggagc uuaucaugua ucuggggguc aaggagaaga ugcuuucgaa     1860

agccaauuaa gcagucauua ugauuucugu aagauucuag gcguagauga acuaagugau     1920

gcagacgaag cuauggcaga agacaucaua uuauggaaca ccguuuuuga agacagaagu     1980

auacucaaac ggaaaauaaa acuagccuac ggagacaggu ugggagacgc acagguaaga     2040

aaacucuguc agaaaaggua uaccggaugg ggcagauuga guggugaacu acuacgcgga     2100

cuuaggucag ccacagauaa ugguccaaaa acaauaaugg auauuuuguu agaaggggau     2160

ccuaaccaug caagaaggau gggugcagcg augaaucuua uggaaauccu ccacgaugau     2220

uugcuagagu uucaagcaaa aaucgaugaa cauaauagag agagagcacu agcgguaggu     2280

ggcaugagcg uugaugaggu gacggggagu ccggcguuaa gaagaggaau aaaucaagcg     2340

cuacguguag uugaugaaau cgcaggaauu gcaggcaaac cacccgcaca uauauacgug     2400

gaaguaacuc gagaugaagg agcaagaaau aaaggacgua gaacaacaag ucgauacaga     2460

gcuuuagaag cagcacuaag uaguaucaaa gcacaaugug cugauguuua ugcagaauua     2520

aaggaaagag aucccagagc acuugaugau gaaagauuga ccuuauauuu uaugcaaugu     2580

gggaagugua uguauucugg agaugcacua gauaucaaca aacuacaaac uuaucaaguu     2640

gaucauauac uaccacaauc auacguaaag gaugauaguu ucgaaaacaa gguacucguc     2700

agaccagaua caaaccaacg aaaaggggau acucuguuau uggaugaaag acucauagca     2760

cgaaugaggg gauuuuggag aacauuaaga gacgcagaau uaauagggga aaagaaauuu     2820

aauaauuuaa cgcgaacaag uguaagugau gaacaaauga agaaauucgu aagcagacag     2880

cuuguagaaa cuggucagau gguuaaacaa guacagcaac uauugcaagc aagauaccca     2940

gagucagaag uacaaccagu aaaagcugga guaucucacc aguuaagggu agggugugca     3000

cuugcaaaaa gcagagaaau uaaugacuuc caucaugcac acgaugcaua ucuggcaugu     3060

caagugggua gauuuguaca guaucgacau ucagcaguau auucagaacc cguuaaaaug     3120

gcagcgguug ugaaacaauu uauaaagaaa caggcagaag auuaucgacg uacucgugaa     3180

cuaccaggua gugauggauu uauuguaagc uccuucauga caccagguuu cgauguaaaa     3240

acaggugaag uauuuagaga ugcaugggau gcuggggcug aaauagaccg aauuagaaga     3300

ugcuuugauu auaaagacug cuuuaucagu agaaugccag aagagaccca cggggcauuc     3360

ugggacgcaa cuauuuacuc accgagagcu gccggaaaaa cuuuaagccu accucuaaaa     3420

agaggucuug auccucgaaa auacggaagu uacaguagag aacaauuugc cuacuuuuuc     3480

guuuacgaag caguaaagcc uaaaaagaaa caaagaguac uagaauuugc uccaguccca     3540

guuagaguug caagcgccuu agcaaaugau ccagaugcac uagaagacua ugcaagggaa     3600

cucgcugaag cggaagggcu aguuuucgaa agaauacgaa ggcgaaaagu uuacaaauau     3660

cagcaaauaa ucguuggaga cuccagauua uauguuacag ggagagaaga agugagaaac     3720

gcccgacaac uagcauucuc acaagacgaa acaaaacuaa uaugucguau cgaacaaggg     3780

gaagaaugug aagaaggcga auuauuagac uuauuuagau ccaugcaaag gaaauaccua     3840

gcgugugcac cgagacucgc ucgauuaaug aaaguugcag aaauggaaga aggauuuauu     3900

gcugcuacag caucuggaaa aagagaagua uuauuaaguu ugaccucaau agcugcagca     3960

caccgaaaua guauugauuu aaaaccaaua ggaagaaaag gaucuucagg acaaaugaaa     4020

aucacuuuua guaaagaauu aucaacaaac gguauacuau ucguugacuc aaguguuacc     4080

gguauguuug aaagacggug uagacuugga uuauga                               4116


<210>  5469
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5469
auggaaaaca aaaacuacag uauaggauua gacaucggaa ccaauucagu gggcuggucc       60

guaaucacag acgacuacaa aguccccuca aagaaaauga aaguauuagg gaacacagau      120

aaacguuuua uaaagaaaaa cuugaucggu gcucucuuau uugaugaagg uacaacagca      180

gaagcacguc gacuaaaacg aacagcccga cgucgcuaua cacgcagaaa gaaccgauua      240

agauaccuac aagagauauu ugccgaagag augucaaaag uggauagcuc uuucuuccac      300

agauuagacg acucuuuccu aaucccugaa gauaaaaggg gaucaaaaua cccgauauuc      360

gcaacacuag cagaagaaaa agaauaucau aaacaauuuc caaccauuua ucaucuaagg      420

aaacaacuug cagacucuaa agagaaaaca gaccuaagac uuauauaucu cgcacuagcc      480

cacaugauua aauaccgagg acacuucuua uaugaagaua cauuugauau aaagaacaac      540

gauauucaga aaaucuuuuc cgaguucauu agcauauaug auaacacauu cgaaggcagc      600

ucauuaagcg gccaaaacgc acaaguagaa gcuaucuuca cugacaagau aucuaaauca      660

gcaaaaagag aacgaguucu caaauuguuc ccagaugaga aaaguaccgg gcuuuucuca      720

gaauuucuaa aauuaauagu cgguaaucaa gcagacuuca agaaacauuu ugacuuaggc      780

gaaaaagcac cauuacaguu uuccaaagac accuacgaug aagaccucga aaacuuacuc      840

ggacagauug gagaugacuu cgcagaucua uucuuaguag ccaagaaacu uuacgaugca      900

auauuauuau cagguaucuu aaccguuaca gauccaucaa caaaagcucc auuauccgca      960

ucuaugauug aaagauacga aaaucaucaa aaagaccuag caguacucaa acaauucauc     1020

aaaaauaauc ugcccgaaaa auaugaugaa guuuucagug accaaucaaa agacggcuac     1080

gcaggcuaua uagaugguaa aaccacacaa gaggcuuucu auaaauacau aaagaaucua     1140

uuaucaaaau ucgaagguac agacuauuuc cuagaaaaga uugaacgaga agacuuuuua     1200

agaaaacaaa gaacauucga uaaugguagu aucccacacc aaauacaccu acaagaaaug     1260

aacgcaaucc ucaggaggca aggugaacac uacccuuucc ucaaggacaa uaaagagaaa     1320

auugagaaaa uacuaacauu ccgaauacca uacuacguag gccccuuagc aagaggaaau     1380

agagacuuug ccuggcuaac uagaaacucc gacgaagcaa uacgcccaug gaacuucgaa     1440

gaaauaguug auaaagccuc aucagcagaa gauuuuauua acaaaaugac caauuacgau     1500

cucuacuuac cagaagaaaa gguuuuaccu aaacacuccc uccuauauga aacuuuugca     1560

gucuauaacg aacuaacuaa aguaaaguuu aucgcagaag guuuaagaga cuaucaauuc     1620

cuagacucag gccaaaagaa acaaauagua acgcaauuau ucaaagaaaa gcguaaaguu     1680

acugaaaaag acauaauuca auuccuacac aacguagacg gauaugaugg aauugaacua     1740

aaagguauug aaaaacaauu caacgcaucc cugucaacuu accaugacuu guuaaaaaua     1800

auuaaagaca aagaauuuau ggacgacucc aaaaaugaag aaauacucga aaauaucguc     1860

cacacauuaa caauauucga agauagagaa augauuaaac aaagacuagc acaauaugac     1920

ucauuauucg augaaaaagu aauaaaggcu uuaauaagga gacacuauac aggauggggc     1980

aaacuaucug cuaaacuuau cgacgguauc ugugauaaac agaccggcaa uaccaucuug     2040

gacuacuuga uagacgaugg uaaaaacaac agaaauuuca ugcaacucau aaacgacgac     2100

ggauuaucau uuaaagaaau cacucaaaaa gcacaggucg uuggcaaaac agaugaugua     2160

aaacaaguag uacaagaacu gccugguagc ccagcuauaa agaaaggaau auuacaaagc     2220

auaaaaauag uagaugagcu aguaaaagua augggccaua caccagaauc uaucguaauc     2280

gaaauggcua gggaaaacca gacaacagca cgcggaaaga aaaacucuca acaacgcuac     2340

aaaagaauag aagaugcccu caaaaaccuc gcuucgggac uagauuccaa cauucucaaa     2400

gaacauccaa ccaacaauau acaacuacaa aacgauagac uguuucuaua cuaccuccaa     2460

aauggaagag auauguauac cggaaaacca cuggauauua accaauuauc cucuuacgac     2520

auagaucaua uaguaccaca agcauucauc aaagaugacu cauuagacaa uaggguucua     2580

accagcuuaa aagauaaccg agguaaguca gacaauguac cuucaauuga aguaguucag     2640

aaaagaaaag cauucuggca acaacuucuu gacagcaaac uaaucucuga acguaaauuc     2700

aacaaccuaa cuaaagcaga gagaggagga cuagaugaac gagauaaagu cggcuucaua     2760

aagcgacagc uaguagaaac uagacaaaua acuaaacaug uagcccaaau ucuggacgca     2820

cgauuuaaua aagaagucaa ugaaaaagac aagaaaaaua gaacaguaaa aaucaucaca     2880

cuaaaaucca aucugguguc aaacuuuaga aaagaauuuc gacuauauaa aguacgagaa     2940

auuaacgauu accaucacgc acacgacgcu uaccuuaaug ccgugguagc uaaagcaaua     3000

cucaaaaagu auccaaaauu agaaccggag uucgucuaug gugacuacca aaaauaugac     3060

cucaagagau acauaagccg uucacgcaac ccuaaagaag ucgaaaaagc uacggaaaag     3120

uacuuuuucu auagcaauuu auuaaacuuu uucaaagaag aaguccauua cgcugaugga     3180

acaauaguaa aaagagaaaa uauagaauau ucgaaagaca ccggagaaau cgcauggaac     3240

aaagagaaag auuuugccac aaucaagaaa guacucucau acccacaagu aaacauagug     3300

aagaaaagag aaguucaaac cgguggauuc ucaaaagaaa gcauacuccc aaaaggaaac     3360

agcgauaaac uaauacccag aaaaaccaaa gacauacuau gggaaacuac uaaauacgga     3420

gguuuugacu caccaguaau agcuuauagu auacuacuca uagcagauau agaaaaaggu     3480

aaagcuaaga aacuaaaaac ugucaaaacc cuaguaggaa uaacaauaau ggaaaaagca     3540

gcguucgaag aaaacccuau uacauuccug gaaaacaaag gauaccacaa cguacgaaaa     3600

gagaauauau uaugcuugcc aaaauacucu uuguuugaac ucgaaaaugg uagaagaaga     3660

uuacucgcau cugcaaaaga acugcaaaaa gguaaugaaa uaguauugcc aguuuacuua     3720

acuacucuuu uguaccauuc caaaaacguc cauaaacucg acgaaccaga acaccuagaa     3780

uauauccaaa aacauagaaa ugaguucaaa gaccuauuaa accuaguaag cgaauuuuca     3840

caaaaauaug uauuagcaga cgcaaaucuu gagaaaauac aaaacuugua ugcugacaac     3900

gaacaagcug acauagaaau ccuagcaaac agcuuuauaa acuuguuaac auucaccgca     3960

uuaggcgcuc cugcagcuuu uaaauuuuuc ggcaaagaca uagacagaaa aagauacaca     4020

accguaucag aaauuuuaaa ugcaacacuc auucaucaaa guauaacggg auuauacgaa     4080

acacgaauag auuuaaguaa auuaggugaa gauuga                               4116


<210>  5470
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5470
auggacaaga aauacucaau aggccuagau auuggaacua auucaguagg uugggcagua       60

auaacugacg acuacaaagu uccuucaaag aaauucaaag uacuaggaaa cacagacaga      120

cacucaauaa agaaaaaucu gauaggugcc uuguuauuug auaguggaga aacggcagaa      180

gcaacuagac uuaaacggac agcacgccga cguuauacca gacguaaaaa cagaauaaga      240

uaucuucaag aaauuuucag uucggaaaug ucaaaaguag augauucauu cuuucauaga      300

cucgaagagu cuuuucuagu agaagaagac aagaaacaug aaagacaucc aauauuuggg      360

aacauaguug augaaguugc uuaccacgaa aaauauccaa caauauacca uuuacgaaag      420

aaacuggcag acucgaccga uaaagcugac cuaagauuaa uauaucuugc acuagcacau      480

augauuaaau uuagaggaca uuuucuaaua gaaggagacc uuaauccgga caacucagau      540

auggauaaac uauucauaca gcuaguacaa acauauaauc aauuauucga agaaaauccu      600

auaaaugcuu cucgaguaga cgcuaaagca auacuaucag cucguuuaag caaaaguaga      660

cguuuggaaa accuaauugc acaacugcca ggagaaaaac gaaacggauu auucggaaac      720

cucauugcuc uaucccucgg uuuaacacca aauuuuaaau cuaauuuuga uuuagcagaa      780

gaugcaaaau uacaacuaag caaagacaca uaugacgacg accuugauaa ucuacuagcc      840

cagauaggag accaauaugc agacuuauuc cucgcagcua aaaaccucuc agacgcaaua      900

uuauuaucag auauacucag aguuaauucc gagauaacua aagcuccacu aucagcaagc      960

augauaaaaa gauaugacga acaucaucaa gaucugacac uucuaaaagc ucuugucaga     1020

caacaauuac cugaaaaaua uaaagaaaua uucuuugauc aaagcaaaaa uggauacgca     1080

ggauacauag acggaggugc gucacaagag gaauuuuaca aauuuauaaa accuauccua     1140

gaaaagaugg acggcacaga agaauuacua gcaaaacuca auagagaaga ccuacuccga     1200

aaacagcgaa cauuugauaa cggcucaauu ccucaccaaa uccacuuagg agaauuacac     1260

gcaauccuua gacgccaaga agauuuuuac ccauuuuuaa aagauaacag agagaaaauu     1320

gaaaagauau uaacauuucg cauaccuuac uaugucgguc cucuggcgcg aggcaauuca     1380

cgcuuugcgu ggaugacaag aaaaagugaa gaaaccauca cucccuggaa cuucgaagaa     1440

gucguagaua aaggcgccuc agcacagucg uuuauagaaa ggaugaccaa cuuugauaaa     1500

aauuugccua augaaaaagu ucuaccaaaa cacucuuuac uauaugaaua uuucacugua     1560

uacaacgaau uaacaaaagu aaaauaugug acggaaggca ugagaaaacc cgaguucuug     1620

ucaggaaaac aaaaagaagc cauaguggau uuauuauuua agaccaacag aaaagucacg     1680

gucaaacaau uaaaggaaga cuauuuuaag aaaauagaau guuucgauuc aguagaaaua     1740

agcggcguug aagacagauu uaaugcuucc cuaggaacau accaugaucu acuaaaaauu     1800

auaaaagaca aagauuucuu agacaaugaa gaaaacgagg auauuuuaga agacauagua     1860

cuaacucuaa cacuauucga agacaaagaa augauugaag aacgccuaaa gaaauacgca     1920

aacuuauuug acgauaaagu aaugaaacaa cugaaacgaa gacauuauac aggauggggc     1980

agauuaucaa gaaaacucau aaauggaaua cgagauaagc aaucagggaa aaccauauua     2040

gacuuucuca agagugaugg auuugcaaau agaaacuuca ugcaguuaau aaacgaugac     2100

agccuaacuu uuaaagaagc cauacaaaaa gcccaaguaa guggucaggg acauucacuu     2160

caugagcaaa uugcuaaccu agcagguucu ccagcaauaa agaaaggcau uuuacaguca     2220

gucaaaguag uagacgaacu gguuaaagua augggacaca aaccugaaaa cauagugaua     2280

gaaauggcuc gagaaaacca aacuacgcaa aaaggacaga aaaacagcag agaaaggaug     2340

aaaagaauag aagaaggaau aaaagaacug ggaucacaga uauuaaagga acacccaguc     2400

gaaaacacgc aauuacaaaa ugaaaaguua uacuuauacu auuuacaaaa cggacgagau     2460

auguacgugg aucaggaacu agacauaaau cgacuauccg acuacgaugu agaucauaua     2520

guuccccaau cauuuauaaa agaugacagu auugauaaua aaauacuaac aagaagcgac     2580

aaaaaucgag gaaaaucaga caacguaccu ucagaagaag uaguaaagaa aaugaaaaau     2640

uacuggcgac aauuauuaaa cgcuaaacuc auuacacaga gaaaauucga caaccuaaca     2700

aaagcugaaa gagggggauu gucggaguua gauaaagcag gauucaucaa gcgacaauua     2760

gucgaaacac gacaaauaac gaaacaugua gcacaaauau uggacucacg aaugaacaca     2820

aaauacgacg aaaaugauaa acuaauaaga gaagugaaag ucauaacccu caaaaguaaa     2880

cucguaucag auuuccguaa agauuuucaa uuuuauaaag ugagagaaau aaauaacuac     2940

caccacgcac acgacgcaua uuuaaaugcu gugguaggca ccgcacuaau aaagaaauau     3000

acuaaacuag aaagcgaauu uguauauggu gauuauaaag uauaugaugu acgaaaaaug     3060

auagcaaaau cagaacaaga aauagguaaa gcaaccgcca aaagauucuu uuauucaaau     3120

auaaugaacu uuuucaaaac cgagauuacc uuggcaaacg gggagauaag gaaaagacca     3180

cuaauugaga caaacgaaga aacaggggag auaguuuggg acaaaggaag agauuuugcu     3240

acaguacgaa aaguucuaag uaugccacaa guaaauaucg ucaagaaaac cgaaguccaa     3300

acaggugcuu uaacaaauga aucgauauac gcacguggga gcuuugauaa auuaauuucg     3360

agaaaacaua gauucgaauc aucuaaauac gguggauuug gguccccaac aguuacauau     3420

agcguguugg ucguagcuaa aucaaaaguc caagacggca aagucaagaa aauaaaaaca     3480

ggaaaagaac ucauaggaau gacucuauua gacaaauuag ucuuugagaa aaauccacug     3540

aaauuuauug aagacaaagg uuauggaaac guacaaauag acaaaugcau uaaacugccc     3600

aaauauucgc uauuugaguu cgaaaauggc acaaggcgua ugcuagcgag cgucauggca     3660

aacaacaacu cccguggcga ucuacaaaaa gcuaaugaaa uguuuuuacc cgcaaaauua     3720

guuacccuac uauaucacgc acauaagauu gaaagcagua aagaacucga acaugaagca     3780

uacauauuag aucauuacaa cgaccuauau caauuauuaa gcuauauaga acgguucgcu     3840

ucacuauacg uugacguaga gaaaaauaua ucaaaaguaa aggaacuauu caguaauaua     3900

gaaagcuauu cuauaucaga aauauguagu ucuguuauua auuuacuaac cuugacugca     3960

agcggggcuc cagcagacuu caaauuucua gguacaacca ucccgagaaa aagauacggc     4020

aguccacaau cuauccucuc uucaacauua auacaucaaa gcauuaccgg auuauacgaa     4080

acgaggauag auuuaucaca auuaggaggu gacuga                               4116


<210>  5471
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5471
auggauaaga aauauucuau aggacuagau auaggcacaa acagugucgg augggcaguc       60

auaaccgaug auuauaaagu gccauccaag aaauucaaag uacuggggaa cacagacaga      120

cauagcauca agaaaaaccu uauaggugcc cuauuauucg auucaggaga aaccgccgaa      180

gcaaccagau uaaaacggac ugcaagacgu agguacacuc gacgcaaaaa cagaauaaga      240

uacuuacaag aaauauucuc uagcgaaaug ucaaaaguag acgacuccuu cuuucauaga      300

cuagaagaau cauuucuagu agaagaagau aagaaacaug aaagacaccc cauauuuggu      360

aauauaguug augagguugc uuaucaugaa aaauacccca caauauauca cuuaagaaag      420

aaauuagcgg auucuacuga caaagccgac cuucgauuga ucuaccuugc ccucgcucau      480

augauuaaau ucagagguca uuuucuuaua gaaggagacc ugaauccuga uaacucagac      540

gucgauaaac uuuucauaca auuagugcaa acuuacaacc aacuauucga agaaaaucca      600

auaaacgcaa guagggugga cgcuaaagca auacucuccg cuaggcuuag uaaaucacgu      660

agauuagaaa accucaucgc acaauuacca ggagagaaaa gaaacggacu cuuuggcaac      720

cuaauugcac uaucgcuagg acuaacacca aacuucaaau caaauuuuga cuuagccgaa      780

gacgcuaaac uacaauuauc uaaagacacg uaugaugacg auuuagauaa cuuacucgca      840

cagauugggg accaguacgc ugaucuauuc cuagccgcua aaaauuuauc agaugcaaua      900

cuacuuagcg acauacuucg cguaaauucu gaaauaacua aagcaccacu aucugcaucu      960

augauuaaaa gauacgauga acaccaucag gaucucacau uauuaaaagc ccuaguacga     1020

caacaacuac cugaaaaaua caaagaaauc uucuucgacc aauccaaaaa uggauacgca     1080

ggauacauag augguggagc aucccaagaa gaguucuaca aauuuauuaa accaauacuc     1140

gaaaagaugg augggacuga agaacuguua gcaaaauuaa auagggaaga uuuauuacga     1200

aaacaacgga cuuucgauaa cggaagcaua ccccaucaaa uacauuuagg agaacuucau     1260

gcaauauuaa gaagacaaga agauuucuau cccuuccuua aagacaacag agagaaaaua     1320

gagaaaauuu uaaccuucag gauuccauac uacguaggcc cacucgcuag agguaauuca     1380

agauucgcau ggaugaccag aaaaagcgaa gaaaccauaa caccauggaa cuucgaagaa     1440

guagucgaca aaggagcauc agcacaaagu uuuauugaaa ggaugacaaa cuuugacaaa     1500

aacuuaccua augaaaaagu uuuaccaaag cauagucuau uauacgaaua cuuuacuguu     1560

uacaaugaac uaacaaaagu uaaguauguu acugaaggaa ugcgcaaacc agccuuucuu     1620

ucgggagaac aaaagaaagc gauaguugac cucuuauuua aaacaaauag aaaaguaaca     1680

guaaaacaau uaaaagaaga uuacuucaaa aagauagaau guuucgacuc uguagagauc     1740

uccggugugg aggaccgcuu uaacgcuagc cuagguacuu aucacgauuu auuaaaaaua     1800

aucaaagaca aagacuuucu agacaaugaa gaaaacgaag auauacuaga agacauaguu     1860

cuaacccuaa cacuauucga agacaaagaa augauagaag aacgcuugaa aaccuaugca     1920

cacuuauucg augacaaagu uaugaaacaa cuuaaaagga ggcacuauac uggaugggga     1980

agauugucca gaaaguuaau caauggcauc cgagauaaac aaucggguaa aacuauacuu     2040

gauuuucuaa aauccgaugg cuucgcaaau cguaauuuca uacaguuaau acaugacgau     2100

agcuuaaccu ucaaagaagc aauacaaaaa gcucaaguaa guggucaagg acacucacua     2160

caugaacaga ucgccaaucu agcaggcuca cccgcuauca agaaaggcau ucuacaaagc     2220

gucaaaguag ucgaugaacu cgucaaagug augggccaca aacccgaaaa cauuguaaua     2280

gaaauggccc gcgaaaacca gaccacccaa aaaggacaga aaaacagcag agaaagaaug     2340

aaaagaauag aagaagggau uaaagaacua gguagccaaa uccuaaaaga acacccggua     2400

gaaaacacac aauuacagaa ugaaaagcua uaccuauauu accuccaaaa uggaagagac     2460

auguauguag aucaagaacu agacauaaac aggcuuucgg auuaugaugu ugaccacaua     2520

guaccacaaa guuuuauaaa ggacgacagc auugacaaua aaguuuugac aagaucugac     2580

aaaaaccgag gcaaaagcga caaugucccc ucagaagaag uaguuaaaaa gaugaaaaau     2640

uauuggagac aacuacuaaa ugcaaaacua aucacucaac gaaaauucga caacuuaaca     2700

aaagcagaac gaggaggccu aagugagcuc gauaaagcag guuuuauaaa aagacaacug     2760

guagaaacac ggcaaauuac caaacacgua gcacaaauuu uagacucuag aaugaauacc     2820

aaauacgaug aaaaugauaa acuuaucaga gaaguaaaag uaauaacauu aaaaucuaaa     2880

cuuguuucug acuuucgaaa agauuuccag uucuacaaag uacgagaaau uaauaacuau     2940

caccaugcac augaugcuua ucuaaaugcu guagucggaa cagcacuuau aaagaaauau     3000

ccaaaacuag aaucagaauu ugucuauggu gacuauaaag ucuacgacgu aagaaaaaug     3060

aucgcaaaau cagaacagga aauuggaaaa gcaacagcca aaagguucuu cuacuccaau     3120

aucaugaacu ucuucaaaac agaaauaacu cuagccaacg gcgaaauuag aaagagacca     3180

uugaucgaaa cuaaugaaga aaccggcgaa aucguauggg acaaaggaag agacuucgca     3240

accguucgga aaguauuaag caugccacaa guaaacauag uaaagaaaac cgaaguccaa     3300

accggugccu uaacuaauga auccauauau gcaagaggcu ccuuugacaa acugauauca     3360

agaaaacaua gauucgaauc aucuaaauac ggaggauucg gcucaccaac cgugacguac     3420

ucaguccuag uaguagcaaa auccaaagua caagacggaa aggugaagaa aauaaaaaca     3480

ggaaaagagc uuauaggaau aaccuuauug gacaagcuag uuuucgagaa aaacccuuua     3540

aaauucauag aagacaaagg uuauggaaac guacaaaucg auaaauguau aaaauuaccu     3600

aaauauucac uuuuugaauu ugaaaacggc accagaagaa ugcuugccag uguuauggcu     3660

aauaacaauu caagaggaga ucuacaaaaa gcaaaugaaa uguuuuuacc agcaaagcua     3720

guuacacuac uauaccacgc acauaaaauc gaaucaucua aagaacuaga acacgaggcu     3780

uauauauuag accauuacaa cgacuuguau cagcuauuau cguauauaga acgcuuugca     3840

agucucuacg uagacguuga gaaaaacaua ucaaaaguca aagaauuguu uuccaauaua     3900

gaaaguuacu caauaaguga aauuuguucc ucaguaauaa accuccucac acuaacugcg     3960

ucaggagcuc cugcagacuu caaauuuuua ggcacgacca uuccuagaaa acgauacggu     4020

uccccacagu caauauuguc uaguacacua auacaucaau ccaucaccgg cuuauaugag     4080

acaagaaucg accuuucaca auuaggagga gauuga                               4116


<210>  5472
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5472
auggaaaaca aaaacuacuc cauaggucuc gacauaggaa caaauucagu uggaugguca       60

guuauaaccg acgauuauaa aguuccaagc aagaaaauga aaguauuagg aaauacagau      120

aaacauuuua uaaagaaaaa uuuaauagga gcauuauuau uugacgaagg uacaacagcu      180

gaagcaagaa gauuaaaacg aacugcacgc cgaagauaua caagaaggaa aaauagacuc      240

agauaccuac aagaaauauu uuccgaagaa auagguaaag uggauucuuc auuuuuccac      300

agacuagacg acucauuucu cauaccagaa gacaaaagag guaguaaaua cccgauauuu      360

gcuacucuug ccgaagaaaa gaaauaccac aaacaauucc ccaccauaua ucaccuaaga      420

aaacaacuug cagauucuaa agagaaaacc gaucuaagac ucaucuaccu cgcacuugca      480

cacaugauaa aauaccgugg ucacuuucuu uaugaagaag cauucgauau caaaaacaau      540

gacauccaga aaaucuucaa ugaauuuauu ucaauauaca auaauacauu ugaaggaaac      600

aguuuaagug gacaaaaugu acaaguugaa gcaauauuca cagacaaaau aucaaaaucu      660

gcaaaaagag aaagaguccu aaagcuauuc cccgacgaaa agaguaccgg gcuauuuagu      720

gaauuucuaa aacuaauugu aggcaaucaa gcugauuuca agaaacacuu ugaucuagaa      780

gaaaaagcac cccuccaauu cucaagggau acauaugacg aagacuuaga aaaucuccuc      840

gggcaaaucg gcgaugauuu cgcggaccua uuuuuaguug caaagaaacu auacgaugca      900

auucuauuau cuggcaucuu aaccgugacc aacccaucaa caaaagcacc uuuaucagcu      960

aguaugauug aaagauacga aaaccaucaa aaagaccuag caucuuuaaa acaauucauc     1020

aaaaauaacc uucccgaaaa auaugaugaa gucuucucag aucaaaguga agacggcuac     1080

gcaggauaua uagauggcaa aacaacgcaa gaaacauuuu auaaauacau caaaaaucua     1140

uuaucgaaau ucgaaggcgc agauuacuuu uuagauaaaa uagaacggga agauuucuua     1200

cguaaacaac gaaccuuuga uaauggaagu auaccccacc aaauacaccu ucaagaaaug     1260

aaugcuauuc uaagaagaca aggcgaacac uauccuuucu uaaaagagaa caaagagaaa     1320

aucgaaaaga uacuaacauu ucguauccca uauuauguug guccucuagc aagaggaaac     1380

agggacuuug cauggcuaac cagaaauuca gaucaagcaa uacgccccug gaacuucgaa     1440

gaaauaguug auaaagcguc gucagcugaa gacuucauca auaaaaugac aaauuacgac     1500

cuguauuuac cagaagaaaa aguacuacca aaacacagcc uauuauacga aacuuucgcc     1560

guguauaacg aacuaacaaa agucaaauuu auugcugaag gccuaagaga uuaucaauuc     1620

cuagacuccg gccaaaagaa acaaauagug acgcaacucu uuaaagagaa aagaaaaguu     1680

acagaaaaag auaucauaca auuccuacac aacgucgaug gcuacgaugg cauagaacuc     1740

aaaggaauug aaaaacaauu uaacgcaagu uuguccacuu accacgaucu auuaaaaaua     1800

auuaaagaua aagccuuuau ggacgaugcc aaaaaugaag cuauccucga aaacauugua     1860

cacacuuuaa ccaucuucga agauagagaa augauaaaac aaagacuugc ucaauacgau     1920

agccuauucg acgaaaaagu aauaaaagca cugacacgac gacauuauac aggauggggc     1980

aaacugucgg cuaaauuaau uaacggcaua ucagauaaac aaacaggaaa uaccauauua     2040

gauuaucuaa uagacgaugg agaaaucaac aggaauuuca ugcaguuaau aaacgacgac     2100

gggcugucuu ucaaagaaau aauccaaaaa gcacaaguag uagguaaaac aaacgaugua     2160

aaacaaguug uccaagaacu acccgguagc ccugcaauca agaaagggau auuacaaucu     2220

auuaaaauag uagaugaacu cgucaaggug augggccaug ccccagaaag cauaguaaua     2280

gaaauggcgc gugaaaacca aacgacagca agagguaaga aaaacucuca gcaaagauac     2340

aaacgcauag aagacagcuu aaaaauauua gcaucugguu ugaacaguaa aauacucaaa     2400

gaacacccaa ccgacaauau acaacuacaa aacgacagac uauuccucua uuaucuacaa     2460

aacggaaaag auauguacac aggagaagca uuagauauca accaacuauc gucaugugau     2520

aucgaccaca ucauaccaca ggcuuucaua aaggaugauu cacuagacaa ccguguacua     2580

accucgucaa aagacaaccg cggcaaaucu gauaauguac caaguuuaga aguugucgau     2640

aaaaugaaag uuuucuggca acaacuccuu gauaguaaac uaauaagcua ccgaaaauuc     2700

aacaaucuua ccaaagcaga acgaggcggg cuaaaugagc uggauaaagu aggauucauu     2760

aaacgacaac ugguugagac ccgucagaua acaaaacacg ucgcacaaau acucgaugca     2820

cgauucaaua aagaaguaac ugagaaagac aagaaaaaua gaacaguaaa aauuauaacc     2880

cuaaaaucaa acuuaguauc caacuuuaga aaggaguucg aauuauacaa aguuagagaa     2940

auaaaugauu accaccacgc ucacgaugcc uaccuaaacg caguagucgc uaaagcuauu     3000

cugaagaaau auccaaaauu agaacccgag uucgucuaug gagacuauca aaaauaugau     3060

cuaaaaagau acauaagcag aagcaaggac cccaaagaaa uugaaaaagc aacugaaaaa     3120

uauuucuucu auagcaaccu gcucaacuuc uucaaagaag aaguacacua ugcagaugga     3180

acaauaguca agagagaaaa caucgaauac aguaaagaca caggugaaau cgcauggaau     3240

aaagaaaaag auuucgcaac aaucaagaaa guccuaaguc uaccucaagu aaauauaguc     3300

aagaaaagag aaguacagac uggaggauuc agcaaagaau caauacuacc aaaaggaaau     3360

ucagacaaac uuauaccaag aaaaaccaaa gacauucuuu gggauacaac uaaauacggc     3420

ggguucgaua gcccggucau agccuacuca auucuucuca uagccgacau cgaaaaaggu     3480

aaagccaaga aacuuaaaac cguuaaaaca cucguaggaa uaaccauuau ggaaaaagau     3540

gccuuugaga aaaacccuau cgcauuccua gaaaauaaag guuaucacaa uguuugcaaa     3600

gaaaacauac uuuguuuacc aaaauacuca cuguuugaac uagaaaaugg acguagaaga     3660

uuguuagcaa gcgcaaaaga gcugcaaaaa ugcaacgaaa uaguccuacc aguuuacuua     3720

acuacacucu uauaucauag caaaaacguu cacaaacuug augaaccggg ucaccuugag     3780

uauauacaaa aacacagaaa ugaauuuaaa gacuuauuaa aucuaguauc agaguucuca     3840

caaaaauaug uacucgcaga cgcuaacuua gagaaaauua aaaaucuuua ugccgauaac     3900

gaacaagcag acauagaaau auuggcgaac uccuucauca auuuguuaac uuuuaccgcg     3960

uuaggagcuc cugcagcauu caaauucuuu ggaaaagaug uagaccgaaa aagauacacg     4020

accgucagcg aaauccucaa cgccacacua auucaccaau caaucacugg auuauacgaa     4080

acccgcauag accuaucaaa auuaggagaa gacuga                               4116


<210>  5473
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5473
augaaaguaa auaauuauca cauaggccua gauaucggaa cauccucuau aggcuggguu       60

gcaauuggag aagacggaaa accacuacgc auuaaaggua agacagccau aggagcaaga      120

cuauuucaag aaggcaaccc agcagcugac agacgaaugu uuagaacgac ucgaagaaga      180

uuaucucgaa ggaaauggag acuuaaacua cucgaagaaa uauuugaucc guacauuacu      240

ccaguugaua gcaccuuuuu ugcaagauua aaacagucaa acuuaucacc caaagacuca      300

agaaaagaau ucaagggaag caugcuauuc ccggacuuaa cagacaugca guaccacaaa      360

gacuauccaa ccauauauca ucuuagacac gcacucauga cccaagacga aaaauuugac      420

auucgaaugg uauaccuugc aauacaucac aucgucaagu accgcggaaa cuucuuaaac      480

ucaaccccag uagauagcuu uaaagccuca aaaguaaauu ucguagacca auucaagaag      540

cuaaacgaac uauauacagc aauaaauccc gaagaaucau uucaaaucaa uuuagccaac      600

agugaagaua ucggucacca auucuuagac ccuucaauaa gaaaauuuga uaaaaaaaaa      660

caaaucccca agauaguacc gguaucagua gacgacaaag cgacugacaa aauaaacgga      720

aaaauagcaa gcgagaucau uaaugcaaua uuagguuaca aaucaaaauu agacgugguc      780

gugcaaugua caccggucga cucaaaauca ugggcccuga aauuugauga agaagacauu      840

gacgcuaaau uacaaaaaau auuaccagaa auggaugaaa accaacagag uaucauagcu      900

auacuccaaa acuuauacag ucagguaaca cuaaaccaaa uuguacccaa cgguaugagc      960

cuguccgaau caaugauuga aaaauacaac gaucaucaug accacuuaaa acuauauaaa     1020

aaaauaauag aucaacuugc ugauccaaaa aaaaaagcag cucuaaaaaa agcauauagu     1080

caauacguug gggaugacgg caaaguuauu gaacaagcag acuucugguc aaguguuaag     1140

aaaaaccuug acgauucaga ucuaucaaaa cagauaaugg accuuauuga cgcagagaaa     1200

uucaugccca aacaacgaac aucccaaaau ggggucauac cacaccaacu acaucaacga     1260

gaauuagacg aaauuauaga acaucaaagu aaauacuacc cauggcuagc cgagauaaac     1320

ccaaacaaac augaccucca cuuggccaaa uauaaaauag aagaacuagu agccuuccga     1380

gucccuuauu acgucggacc aaugaucaca ccggacgacc aagcuaaauc agcggaaaca     1440

guauuuucuu ggauggaaag aaaaggaaag gaagcaggac aaaucacucc cuggaacuuc     1500

gacgaaaaag ucgauagaaa ugcaucagcc aaccgauuca ucaaaagaau gacaacaaaa     1560

gacacauacc uaauaggaga agacgugcua ccagacgaaa guuuacuaua cgaaaaauuc     1620

aaaguacuaa acgagcuuaa caugguaaga guaaauggaa aacuauuaaa aguggccgac     1680

aaacaagcaa ucuuccaaga ccuuuuugaa aacuacaagc acauaagugu aaaaaaacuu     1740

caaaacuaua uaaaaaguaa aacaggccua ccaagcgacc ccgaaaucuc aggguuguca     1800

gauccagaau acuucaacaa cagccuaggc acauauaaug acuuuaaaaa acuguuugga     1860

aacaaaguag acgaacccga ucuucaagau gacuucgaaa aaauaguaga guggucuacg     1920

gucuuugaag acaaaagaau auugagagaa aaacuaaacg aaauaaccug gcuaucagau     1980

caacaaaaag auguauuaga gucaucacgc uaucaaggau ggggaagacu uaguaaaaaa     2040

uuauuaacag gcauaguaaa ugaucaagga gaacggauca uagaugaguu auggaacaca     2100

aauaaaaacu ucaugcaaau acaaucagau aacgauuuug ccaaaagaau ccaugaggcu     2160

aaugcggacc aaaugaaagc agucgauguu gaggacguau uagcagaugc auauacaucc     2220

ccccaaaaca agaaagcaau aagacaaguu guaaagguag uagacgauau ucaaaaggca     2280

augggcggag uggccccgaa auauauaucc aucgaguuca cucggucuga ggaucggaac     2340

cccagaagaa ccauaucucg acaaagacaa uuagaaaaca cauugaaaga uacagcaaaa     2400

agucuagcca aaucgauaaa uccagaacua cuaagugaac uagacaacgc agcuaaauca     2460

aagaaaggac uaauugauag gcucuacuug uacuucacac aacuaggaaa agauauauac     2520

acagguaaac caauaaauau cgacgaaauu ucaaccuaug auauagauca caucuuaccc     2580

caggcauucg uuaaagauga cagucucaac aacagaguac uuguuucaaa agcgaucaac     2640

aaugguaaau cagacaaugu ccccguacaa cuauuuggug cuaaaauggg acauuuuugg     2700

aaacaacugg cagaagcagg uuuaaucagu aaaagaaaau uaaaaaaucu ucaaaccgau     2760

cccgacacca uaucgaaaua cgccaugcac ggauuuauuc guagacaacu agucgaaacc     2820

ucacaaguaa uaaaacuagu ugccaacauu uuaggcgaca aauauagaaa cgacgauacu     2880

aaaauaauag aaauaacagc uagaaugaau caucaaauga gagacgaguu uggcuucaua     2940

aaaaauagag aaauaaacga uuaucaucau gcguucgacg cauaccugac ggcauuccua     3000

ggaagauauu uauaucauag auauauaaaa cuacgaccau acuuugucua cggagacuuc     3060

aaaaaauuca aagaagauaa aguaaccaug agaaacuuua acuucuuaca cgaccucacu     3120

gacgacacuc aagaaaaaau agcugaugca gaaacaggag aaguaauuug ggaccgagaa     3180

aauucuauac aacaguugaa ggacguuuac cacuauaaau ucauguuaau uucacacgaa     3240

guauacacau uaagaggggc aauguucaau caaacaguau acccggcauc cgaugcaggc     3300

aagagaaaac uaauaccaau aaaagcagac agaccaauua auguauaugg aggauauagc     3360

gguagugccg acgcauacau ggcuauuguc agaauacaua acaaaaaagg cgauaaauau     3420

cgaguagucg gaguuccuau gagagcaaga gacagauuag augcugcaaa aaaaguauca     3480

gacgcagacu acgacagggc acuaaaagac guauuaacac cacaauuaac caaaacaaaa     3540

aaaaguagaa aaacagguga gauaacacaa gucguagaag acuucgaaau ugucuuaggu     3600

aaaguaaugu auagacaacu aaugauagac ggugacaaaa aauuuaugcu aggcucuucc     3660

acauaucaau acaaugcuaa gcaauuagua uugagcgauc aaucuguuaa gaccuuagca     3720

ucuaaagguc gacuugaucc ccugcaagag ucuauggauu acaauaacgu uuauacagaa     3780

auacucgaua aaguaaacca auacuucuca cuauacgaca ugaacaaauu cagacacaaa     3840

uuaaaccucg gauuuucaaa auuuauauca uuuccaaacc acaacguauu ugacggaaau     3900

acaaaagccu cguccggaaa aagagaaauu cuagaagaag uauuaaaugg acuacacgca     3960

aacccaaccu ucggaaaccu caaagacaua ggaauaacca caccuuucgg acagcuccaa     4020

caaccaaacg gaauccuacu aucagaugaa gcuaaaauca gauaucaauc uccaaccggu     4080

cucuuugaaa gaacaguauc ucucaaagau cucuga                               4116


<210>  5474
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5474
augaaaguaa acaacuauca uauaggccug gauaucggca caaguucuau cggcugggua       60

gcaauaggaa aagauggaaa accucuaaga guaaaaggaa aaacagccau uggagcacgc      120

cuauuccaag aggguaaucc agccgcugac cgacguaugu uuaggacaac cagacgucga      180

cucuccagga gaaaauggcg acuaaaauug cucgaagaga ucuucgaccc auauauaaca      240

cccguugacu ccacauucuu ugcaagacua aagcaaucaa aucuaagccc aaaagacagu      300

agaaaagaau uuaaaggauc aauguuauuu ccagaccuua cagacaugca auaccauaaa      360

aauuauccca cuaucuauca ucuaagacac gccuuaauga cccaagacaa gaaauuugac      420

auacguaugg ucuacuuagc aauucaccau aucguuaaau auagagggaa cuuuuuaaac      480

agcacuccag uugauucauu caaagcaucu aaaguagauu ucguagauca auucaagaaa      540

cuaaacgaau uauaugcggc cauaaaccca gaagaaucuu ucaaaaucaa ccuugcgaau      600

agcgaagaca uaggucacca auuccuagau ccauccaucc gaaaauuuga caaaaagaaa      660

cagaucccaa aaauaguacc aguaaugaug aaugacaaag uuacagaccg auuaaacgga      720

aaaaucgcga gcgaaaucau acacgccaua cuaggauaca aagcaaaacu agacguagug      780

cuacaaugua caccaguaga uucaaaacca ugggcauuaa aauuugauga cgaagacaua      840

gaugcuaaac uagagaaaau ccuacccgaa auggacgaaa aucagcaauc cauaguagcu      900

auucuccaga auuuauacuc acaaguaaca uugaaucaaa uagucccaaa ugguauguca      960

uuaagcgaaa gcaugaucga aaaauacaau gaucaucaug aucaucuuaa acuauacaag     1020

aaauuaaucg accaacuagc ggacccgaaa aagaaagccg uacuaaagaa ggcuuacagu     1080

caauacguag gagacgaugg caaaguuaua gaacaagcag aauuuugguc aucuguaaag     1140

aaaaauuuag augauuccga acucucuaaa cagaucaugg aucuaaucga cgcugaaaaa     1200

uucaugccaa aacaaagaac uucccaaaau ggagucaucc cucaccaacu ccaccaaagg     1260

gaacuagacg aaauaauaga acaucaauca aaauauuacc cauggcuggu agaaaucaau     1320

ccaaauaaac acgaccuaca ccuagcuaaa uauaaaauug aacaacuggu agcauuucga     1380

guaccguauu acgucgggcc gaugauaacu ccaaaagauc aagcagaaag cgccgaaaca     1440

guauuuucgu ggauggaaag aaaagggaca gaaacaggac aaaucacacc cuggaacuuc     1500

gaugaaaaag ucgaucgaaa ggcuucugca aaccgauuca ucaaacguau gacaacuaaa     1560

gauacauacu uaaucgguga agacguacua ccugaugagu ccuuauugua ugaaaaauuu     1620

aaaguauuaa augaacuaaa uaugguaaga gugaacggca aacuauuaaa agucgcagac     1680

aaacaagcua ucuuccaaga ccucuucgaa aacuauaaac auguuucagu uaagaaacua     1740

caaaacuaua uaaaagcaaa aacaggcuua ccaucagauc cugaaauauc cgguuuaucc     1800

gauccggagc acuucaauaa uagucucgga accuauaacg auuucaagaa acuauucggu     1860

ucaaaaguag acgaaccaga ucuacaagac gauuucgaga aaaucguaga auggucaaca     1920

guauuugaag auaagaaaau auuaagagag aaacuaaacg aaauuacaug guugagcgac     1980

cagcaaaaag augugcuaga aucuucuaga uaucaaggau ggggaagacu uaguaagaaa     2040

uuacuaacag gaauaguaaa cgaucaaggc gagagaauaa ucgacaaacu cuggaacaca     2100

aauaaaaauu uuaugcaaau acaaucugac gacgacuucg ccaaacgaau ccacgaagca     2160

aacgccgacc aaaugcaagc agucgaugua gaagaugucc uagcagaugc cuacacuuca     2220

ccacaaaaca agaaagcgau aagacaaguc guaaaaguag uugaugauau acaaaaagcu     2280

augggaggug uggcuccaaa auacauauca aucgaguuca ccagauccga agacagaaau     2340

cccagacgaa ccaucucuag acaaaggcaa uuagaaaaca cauuaaaaga cacugcaaaa     2400

ucacuagcga aaucaauuaa cccugaacuc cuaucugaau uagauaaugc agccaaaagc     2460

aagaaaggcc uaacagaccg acuauacuua uacuuuacac aacuaggcaa agacauauac     2520

acuggcgaac caaucaacau ugaugaacua aauaaauacg acauagacca uauucuacca     2580

caagcauuua uaaaagauaa uagccuugac aaccgugucc uaguacuuac ggcagugaac     2640

aauggcaaaa gcgauaaugu ucccuugaga auguucgggg caaaaauggg gcacuucugg     2700

aaacaauuag ccgaagcugg ucuuauuuca aaaaggaaau uaaagaaccu acaaacugau     2760

ccugacacua ucucaaaaua ugcuaugcac ggauucauuc gucgacagcu agucgaaaca     2820

agucaaguaa uaaaauuggu agccaauauc uuaggagaca aauaucgaaa cgaugacaca     2880

aaaaucaucg aaaucacagc acgaaugaau caccaaauga gagacgaauu uggguuuauu     2940

aaaaacagag aaauaaacga uuaucaccau gcguucgacg cauacuuaac ugccuuucua     3000

ggaagauauc uauaucaccg cuacaucaaa cuacgaccau acuuuguaua cggugacuuu     3060

aagaaauuua gagaagauaa aguaacuaug agaaacuuca auuuucuuca ugaucuaaca     3120

gaugacacuc aagagaaaau agccgacgcu gaaaccggag aagucauuug ggacagagaa     3180

aacucgauac aacaacuaaa agauguauau cauuacaaau ucaugcuaau aucucacgag     3240

guuuauacuc ucagaggagc aauguuuaau caaacagucu acccagcaag ugacgcaggc     3300

aaacguaaac uaauaccagu gaaagcagac agacccguaa augucuacgg aggcuauuca     3360

ggaagugcag augcuuauau ggcgauagua agaauacaua auaagaaagg cgauaaauac     3420

aggguaguag gugugccaau gagagcucua gauaggcuag acgcggcgaa aaacguuucc     3480

gacgcagacu ucgaccgugc ucucaaagau guucuagccc cacaauuaac aaaaacaaag     3540

aaaucaagaa aaacuggcga aauaacccaa gucaucgaag auuuugagau aguucuagga     3600

aaagugaugu acagacaacu caugauagau ggagauaaga aauucauguu ggguucauca     3660

accuaucaau auaacgccaa acagcugguc uuaagugacc aaaguguuaa aacguuagca     3720

aguaaaggac gacuagaucc acuucaagaa ucaauggauu acaacaacgu auauacugaa     3780

auacuagaua aaguaaauca auacuucucu cuuuacgaca ugaauaaauu ucgccauaaa     3840

cuaaaccuug gauuuuccaa auuuauaucu uuuccgaauc auaacguacu ggauggaaac     3900

acgaaaguau ccucaggaaa acgugaaaua uugcaggaaa uuuuaaaugg ucuucacgcg     3960

aacccaacau uugguaauuu gaaagacgua gguauaacca cgccguucgg ccaguuacaa     4020

caaccaaacg guauucuacu uagugaugaa acaaaaauca gguaucaauc gccaacaggc     4080

cuauucgaaa gaacaguuuc auuaaaagau cuauga                               4116


<210>  5475
<211>  4116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5475
auggacaaaa aauauuccau aggauuagac aucgguacua auagcguugg uugggcugua       60

auaacggacg auuacaaagu gccaucaaaa aaauuuaaag uucuagggaa cacagauagg      120

cacagcauaa aaaaaaaccu cauaggggca cuccuguuug acucaggcga aacagcagaa      180

gcuacuagac uaaaaagaac cgccaggagg agauauacua gaagaaaaaa cagaauaugu      240

uacuuacaag agauauucuc aaaugaaaug gcaaaagucg acgauagcuu uuuccaccgg      300

cuagaagaaa gcuuccuggu agaggaggac aaaaaacacg agcgacaccc uaucuucgga      360

aacauagugg augaaguggc auaucacgaa aaauauccaa cgauauacca ccuaagaaaa      420

aaauuagcag acucgaccga caaagcggac uuaagacuua uuuaucuagc uuuagcacac      480

augaucaaau uuagaggaca cuuucuuaua gaaggagacc uuaaccccga caacuccgau      540

guagacaagc uauuuaucca acugguacaa acuuacaauc aacuauucga agaaaaccca      600

aucaacgcgu cucgugucga cgccaaagca aucuuaucag cgagauuauc uaaaucuaga      660

cgauuggaaa accucaucgc acaauuaccu ggcgaaaaaa aaaacggguu auuuggaaau      720

uuaauagcac ucagcuuagg acucacacca aauuuuaaau cuaacuuuga ccuagcagag      780

gacgcaaaac uccaguuaag uaaagacaca uacgacgacg acuuggacaa ucuauuagca      840

caaauaggug accaauacgc ggaucuauuu uuagcagcaa aaaaucucuc agacgcuacc      900

uugcuaagcg acauccuacg cguaaacucg gaaauaacaa aagcaccuuu auccgccuca      960

augaucaaaa gauaugauga acaucaucag gaucuaaccu uauuaaaagc acuaguaaga     1020

caacaguuac cagaaaaaua caaagaaaua uuuuucgacc aaagcaaaaa ugguuaugca     1080

ggguauaucg acgggggagc cucucaagaa gaauuuuaua aauuuauaaa acccauacua     1140

gaaaaaaugg acggaaccga agaacucuua gcaaaacuaa auagagaaga uuugcuaaga     1200

aaacagagaa cauuugacaa uggcaguaua ccacaucaaa uacaucucgg agaacugcau     1260

gcuaucuuac gaagacagga ggauuuuuac ccauuucuca aagauaaucg ugaaaaaaua     1320

gaaaaaauac ucacuuuuag aauccccuau uacguaggac cauuagcgag gggaaacuca     1380

cgcuucgcuu ggaugaccag gaaauccgaa gaaaccauaa cacccuggaa uuucgaagaa     1440

guuguagaua agggagcaag ugcucaauca uuuauagaaa gaaugaccaa cuucgacaag     1500

aaucuuccaa augaaaaagu uuugccaaag cauucacuau uauaugaaua uuucacaguu     1560

uauaacgaac uaaccaaagu aaaauacgua acugaaggaa ugcgaaaacc agcguucuua     1620

uccggcgaac aaaaaaaagc uauuguagau cuacuauuca agacaaacag aaaaguuacu     1680

gucaaacaau uaaaagaaga cuacuuuaaa aaaaucgaau guuucgauag cgucgaaaua     1740

ucagggguag aagacagauu caaugcaucu uuaggaacuu aucacgaucu acucaaaaua     1800

auaaaagaua aagauuuccu ggauaacgag gaaaaugaag auauacuuga agacauaguu     1860

cucacguuaa cacuauucga ggauaaagaa augauugaag aacgacuuaa gaccuaugcu     1920

caucucuuug acgauaaagu aaugaaacaa cugaagcgga gacacuauac uggcugggga     1980

agacuuucca gaaaacuaau aaaugguaua agagauaaac agagcggaaa aacuauacua     2040

gauuuucuga aaagcgacgg auucgcaaau agaaacuuca uccaauugau acaugaugac     2100

ucacuaacau ucaaagaagc gauccaaaaa gcacaaguau caggacaagg gcacucucuu     2160

cacgaacaaa uagccaauuu agcagggucu cccgcaauca aaaaaggcau acuacaauca     2220

guaaaaguag uagacgaauu agucaaagua augggccaca agcccgaaaa uaucguaaua     2280

gaaauggcaa gagaaaacca aacaacacaa aaaggacaaa aaaauuccag agaaagaaug     2340

aaaagaauug aagaagggau caaagaauua ggcucacaaa uacucaaaga acacccugug     2400

gagaauacac aauuacaaaa cgaaaaacuu uaccuauauu aucuacaaaa ugggcgagau     2460

auguauguag accaagaacu ggauauaaau cgauuaagcg auuaugaugu agaccauaua     2520

gucccacaau cuuuuauuaa agacgauuca auugacaaca agguacugac uagaucagau     2580

aaaaaucgug gaaaaucgga cgacguccca agugaagaag uaguaaaaaa aaugaaaaau     2640

uauuggcgcc aauuacuaaa ugcaaaacua auaacacaaa ggaaauucga caacuuaaca     2700

aaagccgaaa gaggaggauu aagcgaauua gauaaagcag guuucauaaa acgccagcuc     2760

guagaaacca ggcaaauaac aaagcaugug gcucaaauuc uagacucaag aaugaacaca     2820

aaguacgaug aaaacgacaa acuuauaaga gaagucaaag uuauuacacu aaaaucuaaa     2880

cuaguaagcg auuuuagaaa agauuuccaa uuuuauaaag uccgagaaau aaacaacuau     2940

caccacgcuc augaugcaua ucucaaugca guaguaggua cagcauuaau aaaaaaauac     3000

ccgaaauuag agucagaauu uguauacgga gauuacaaag ucuaugaugu aagaaaaaug     3060

auagcaaaaa gcgaacaaga aauaggcaaa gcaacagcua aaagauucuu cuauucaaau     3120

aucaugaacu ucuuuaaaac agagaucacu cuagcuaacg gggaaaucag aaaaagacca     3180

cucauagaaa cuaaugaaga aacaggagag aucguguggg auaaaggaag agacuuugca     3240

acuguaagaa aaguacuauc uaugccacaa guuaacauag uaaaaaaaac ugagguacag     3300

acgggcgcgu ugacaaauga aaguauuuac gcaagagggu ccuucgauaa auuaauauca     3360

cgcaaacaua gguuugaauc cucgaaauac ggcgguuuug gcucaccaac aguuacuuac     3420

uccguauuag uaguagccaa aagcaaaguu caagacggaa aaguaaaaaa aauaaaaaca     3480

gguaaagaac uaauaggaau uacucuacua gacaaacuug ucuucgaaaa aaacccauug     3540

aaauuuauag aagacaaagg auaugguaac guacagauag auaaauguau uaaacuaccu     3600

aaguauuccc uauucgaguu ugaaaacgga acacgacgaa ugcucgcuuc aguaauggca     3660

aauaacaacu ccagaggaga ccuacaaaaa gcaaaugaaa uguuccuacc agcaaaacuu     3720

gucacauugc uuuaccaugc acacaagaua gaaucaucaa aagaacuaga acacgaagcu     3780

uacauacugg accauuacaa ugaucuauac caacuacuau cauacauaga aagauuugca     3840

ucucuauacg uagacguaga aaaaaauaua uccaaaguca aagaguuauu cucaaacaua     3900

gaguccuauu ccauaagcga aauauguucc uccguaauca acuuacuaac acuaacugcc     3960

uccggugccc cugccgauuu caaauuuuua gggacaacca uaccuagaaa aagauaugga     4020

ucuccucaaa guaucuuauc aucaacacuu auacaccaaa gcaucaccgg auuauacgaa     4080

acucguaucg auuuaucaca guuagguucc gacuga                               4116


<210>  5476
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5476
augaacaaug aauacuucgu uggauuugau auagguacua auucgguugg agcggcagua       60

acagaugaaa aauaucacgu acucaaauuc aaaggaaaug cgauguggac uucagcuguu      120

uuugaaagcg cacaacaauc ugccgacaga agauuaaacc gaacugcucg gcgccgccua      180

gaccgaaguc aacaaagacg agaucuuuua caagagcuaa uagcgccagc aauaacaccc      240

guugacccac gcuuuuucga cagaauaaga gaaagcgccu uaugggcgga agacaaaacu      300

acuggcucac gguuuacgua cuuuucagau gaaggauaua cagauaagca auaucacaaa      360

gaguauccaa caauucauca uuuaauaugc ucauuaauga augauccggc accucacgau      420

ccucgcuuag uguauauagc gugcucauau augcucacuc acagaggaca cuuuuuaaac      480

gaagcugaua aagagaaaau agaugauguc cuugauauac gcaggguauu caaugaauua      540

ugcucuuggu ucacgaguua uggaauagaa guaccuuucu ccugcuuucc agaagaguuc      600

gggaauguau uaacugcaaa cagaggcgua caagacagaa cucgcgccuu cuaugaucua      660

uuauuugcag gaaagaaacc caaaccuagc gaagaugauc aaguagacau cgauguccuc      720

auuaaauuaa uuucaggagg uaagauugaa uuaagcaaac uauuucugaa cgaagaauac      780

ucagaacuag aaaacaacaa aauacaacuu agugacgcug gauuugacga aaaggaaccc      840

gaauuaagau cggaauuagg agaugacuuc ggucuaguag cagcugcaaa gaauauuuac      900

gauuggucac uuuuaacagg aguacucgga gaaugcaaga gcauaucaga agcaaaaguc      960

aaaacauacg aaaaacauag ugaggaucua gcagcuuuac ggagggucgu gaggaaguac     1020

cacccugaca aauacaaaga aaucuucaag aaauacgugg uuggaaauua caccaguuau     1080

ucaggcaacg ugaaaucggc gggaaagauc agugaaacca agcuaaaaag auguaaccaa     1140

gcagaguucu gcgacuauuu gaaaucaaag cucaaagacg uuaaacccgc gccaggugau     1200

gaugaaugug aaagagugcu gaaagaaaua ucaaagcaua cauuuugucc uagacaagua     1260

accacugaua acagaguuau cccauaccaa uuauauuaua cagaauuaaa gaaaguuuua     1320

gaaaaugcga aaagauacau uccacaauua aauguuacug augaauaugg aagugcggcc     1380

gacaaaauau uaucacuaau gacauuuaga aucccauauu auguaggacc auuaguaaag     1440

aaaaacgauu caucaaacgc auggauagug cguaggggcg aaggucguau acucccaugg     1500

aauuuugaug aacuuguuga uaaagauaaa ugugaagacg aauuuauaag gagaaugaca     1560

uguaaaugua ccuauauagc aggcaaaaau gucuuaccca aaaauucauu auuauauugc     1620

aaauuuaugg uucuaaauga gauuaacaac auaaagauca acggagaaag aaucucagua     1680

ggagauaaac agaaaauaua uacagaacua uucgaaaaga aacguaaagu uacuguccga     1740

gacauucgga auuuauuaac aagugaaggc agacucaaag caggcgauac uuucggaggc     1800

auagacauaa ccguaaaauc gucacuaaaa ccacaacaug accuaaaaag auuaauggca     1860

gcggguguua uaaccgaagc agaugcggaa gaaaucauug aaagaaucac auguaccacc     1920

gacgucucac gauuaagaac augguuaggu aaauucggau uaucagcggc agaugcuaaa     1980

uacguaucag gacuaaaaua uucagacuuc gggaggcuau caagagaauu uuuaacagaa     2040

guuaacgaug uugauccagc gacaggagaa guuauuaauc caaacauuau uacgaugaug     2100

ugggaaaaua acauaaaccu aauggaauua uuaagucaaa aguacaguua uagaucacaa     2160

auagaaagua ugaacagaga auauuacgcg gcaaauccau uaacauuaga cgaaagaaug     2220

agcgaaaugu auauaccaag ugcuguaaga cgaccaguca aaagaacaau agaaaugaug     2280

aaagaauaca ugcauauaau gagaaaacca cccaaaagaa uauuuauaga aauggccaga     2340

gaauugacag aucuugacaa uaaagguaaa agaacaucau cuagacguaa uacacuauua     2400

gaauuuuuca agaaaugucc ccaagacacc acggaacuaa gagcccaacu agaaaagaaa     2460

uccgacgaug aauuaagauc agagaaauua uauuuauacu uugcacaauu aggaaaaugc     2520

auguauucag gagaaaguau agauauagaa cgacugggcu caaaauuaua ugacguagac     2580

cacaucuuuc cgcaagcuag agucaaagau gacaguauag aaaacaaagu ccuagucuua     2640

uccgaucuua acggagcaaa auccgauaga uacccaauaa augcugacau ucguaauaaa     2700

augcauagcu ucugggacaa acugcacagc auaggacuaa uuggggagaa gaaauaugaa     2760

agacuaacaa gaucaacugg auucacagau gaugaacuag ccggcuuuau aucacgacaa     2820

cuugucgaga caagacagag caccaaagcc gucgcagcau uguuggcuga agaguuccca     2880

gaauccgaga ucgucuaugu aaaggcgggu cuagcuucac aauucaggca ugaauuugga     2940

cuacugaaau gucgagagau aaaugacuua caucaugcaa aagacgcuua ucuaaacauu     3000

guuaugggaa acaucuacca uguaaaauuc acuaaagacc cgaugaacuu uguaaaagaa     3060

uauagaagaa aagaacaagu cuucgauuua aagauccaga gcauguuaac ccacgacgcc     3120

auuagaaaug gggaaacagc auggauagga gauggaaccu ggcuagaaac uguuaaaaag     3180

caaauggaga aaaacaauau cagauacgua cgguauucau uuaggaggaa aggugcacuc     3240

uucaaucuaa ugccagaacg caaaaaucca ucauuacuac caaggaaaaa cgguaaaaau     3300

augguacuac cagcagaaaa auacggagga uacaacaaua cugcggccag cuucuuuagu     3360

cuagucaaau aucauucagc aaaagguagu ggcguuguaa ucauaccugu agaacuaaua     3420

cacgcacaua gauacgaaca ugauagcuca uuugccgaag aauaugcauu acggguguua     3480

gcagauauag uuucucuaaa gaaaggagau guauugcaga gugucgaauu uccccuaggg     3540

aguagaauau uaaaagucaa cacuuuauua gacaucgaug guuucagggc aaacauaaca     3600

ggaaaagacu caggagguaa auaucuaggc auaagcucug caucaucauu aauaauuucc     3660

cagguagaua caacauacau aaaaagaguc auaagcauaa augaaagaau aucugccugc     3720

gagaaaaaca agcaaucgua uaccauacga ccagaauuug auguuauuac aagugaaaga     3780

aauauagaaa uuuacaauau gcuaugugag aagauaagca gcagaccauu uuguauaaaa     3840

uuugcaaaca cuucggacac auucguuuca ggacgagaaa aauucauagc ccuaucgcuc     3900

aaugaacaag uaaaaacuuu aacaaacaug cuuucuguuu uuaaaaccgg ccgugcuuca     3960

gguugugacc uuacuaauau aggcggcucc aagaauacgg caauaguuaa acuaaaugca     4020

guacuaucaa accuuaaaga uuucaaauca guaaggauaa uugaccaaag uccaacaggg     4080

cuauacgaaa aagugucccc aaacuuauua ucucuauga                            4119


<210>  5477
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5477
augacgaaga aaaauuacag cauuggauua gacaucggaa caaacucggu cggaugggcg       60

guaauaacag acgacuacaa aauaccagcu aagaaaauga aaguauuagg aaacacagac      120

aaagaauaca ucaagaaaaa ucuacuaggg gcacucuuau ucgauagugg ggaaaccgca      180

gaagcaaccc gacugaaacg aacagcacga agacgauaua caagaaggaa aaacagacua      240

agauauuuac aagaaaucuu ugcagaagaa auagcaaaag cagauaauag cuucuuucaa      300

agacucgaug aaaguuuucu aacugaugac gacaaagauu uugacuccca ucccaucuuu      360

gguaacaaag cagaggaaga ugcauaucac caaaaauuua guacaauaua ccaucuccgc      420

aaauaccuug cggacagucc cgagaaaagu gaucuacgau uagucuacuu agcauuagca      480

cauaugauaa aauaucgcgg acacuuccua auagaaggug aacugaaagc agaaaacaca      540

gauguacaaa aauuauuuac agauuuugua gaagucuacg accgaaccuu cgacgacagc      600

cauuuaagug aaguaacugu agaugcauca ucgauccuua cagagaaaau caguaaaucc      660

agacgacugg aaaaucuaau agaaucguau ccaaaagaaa agaaaaacac acuauucggu      720

aacuuaauug cgcuuucacu uggacuacaa ccuaacuuca aaacgaacuu caaacucucu      780

gaagaugcaa aauuacaauu cuccaaagac acauacgaag aagauuuaga agaaguauua      840

gagaaaauag gagacgauua ugccgaucug uucacaucag cgaaaaaccu auacgacgca      900

auauuguuaa gcggcauauu gacaguugac gauuccucua caaaagcacc acuaucagca      960

ucaaugauca aacgauacgc agaacaccau gaagaccuag aaaaacuaaa agcauucaua     1020

aaaacaaaca aaccaaaucu guaccaugac auuuuuaaag acgaaacaca aaauggauau     1080

gcuggguaca uagaaaacgg aguaaaacaa gacgaauuuu auaaauaucu aaaaacaaua     1140

cuaucaaaaa ccgauggauc agaauacuuu uuagacaaaa uugaaagaga agacuuuuua     1200

agaaaacaaa gaacguucga uaauggaucc auaccucauc aaauccaucu acaagaaaug     1260

caugcaauac uaagacgaca gggagacuau uaccccuucu uaaaagaaaa ccaagacaga     1320

auagagaaaa uccuaacauu cagaauccca uacuacguag guccauuggc gaggaaaaau     1380

aguagauuug cuugggcaga auaccauagc gacgagaaaa uaacaccaug gaacuuuaac     1440

aaaguaauag acaaagaaaa aucagcagaa aaauuuauua cacguaugac auuaaaugau     1500

cucuaucucc cagaagaaaa agugcugcca aaacauaguc acguauacga aacuuuugca     1560

guauauaacg aacuaacuaa aauaaaauac gugaacgaac aaggaaaaga agccuucuuc     1620

gacagcaaua ugaaacaaga aauauucgac cacguauuca aagaaaaccg aaaaguuacc     1680

aaagaaaaac uucuaaacua cuuagacaaa gaguucccag aauaucgaau ccaagacuua     1740

auaggacuug acaaagaaaa caagagcuuu aacgccagcu ugggaacgua ccacgaccua     1800

aagaaaauac uagauaagag cuuccuagau gauaaaguaa augaagaagu uauugaagac     1860

auaauuaaaa cuuuaaccuu auucgaagau agagaaauga uacaacaacg ccuucacaaa     1920

uacucagaca uauucucgac acaacaacuc aagaaauuag aacggagaca uuacaccggc     1980

uggggacgac uuucauauaa auuaauaaac ggcauaagaa auaaagaaaa uaauaaaacg     2040

auauuggaau acuuaauaga cgacggauau gcgaauagaa acuucaugca gcuaauuaac     2100

gacgacaccu uaccauucaa acaaaucauc aaagacgcac aaauaauagg aaacauagau     2160

gauaucgcag ccguagucca ugaacuccca ggaagcccag caaucaagaa aggaauacua     2220

caaucgauca aaauugugga cgaauuagua aaaguaaugg ggcacaaccc agauaauaua     2280

guaauagaaa uggcaagaga aaaucagaca acaaauagag ggagauccca aagucaacaa     2340

agauugaaga aacuucaaga cucacuuaaa gaauuagguu ccaauauacu caauaaaaag     2400

aaaccuaguu auguagaaga uaaaguagaa aaugugcauc uccaagacga uagauuauuc     2460

uuauauuaca uucaaaacgg aaaagacaua uauacgggug aagaauuaga cauugacaga     2520

cuaucagacu augacauuga ccacauaaua ccgcaagcau ucaucaaaga cgauucaaua     2580

gacaaucggg uccuaacaag cagugccaaa aaucggggaa aauccgacga ugucccuaau     2640

uuagauauug uccgagcaag aaaagcagac uggguaagac uauauaaauc aggacuuauc     2700

uccaagagaa aauucgacaa ucuaaccaaa agugaaagag gaggcuugac agaagcagau     2760

aaagcagguu uuauaaaacg ccaguuagua gaaacccgac aaauaacuaa acacguagca     2820

caaauuuuag acgcaagauu caauaccgaa cacgacgaag augacaaagu cauacggaac     2880

gugaaaguaa uaacuuuaaa aucaaauuua auaucucaau uccgaaaaga uuucaaauuu     2940

uauaaaguaa gagaaaucaa cgauuaucac caugcucacg augccuaccu aaacgcaguc     3000

guagguacag cccuauuaag aaaauacccu aaauuagcac cagaauuugu guauggagaa     3060

uacaagaaau acgauguaag aaaauuaaua acuucuucaa gugauaaagc aaccgccaag     3120

uauuucuucu auucuaaccu aaugaacuuc uuuaagagag uaguacgaua uaguaacgga     3180

agagucauag uaagaccugu aaucgaauau ucaaaggaua caggagaaau aguauggaac     3240

aaagaaacag acuuuagaac aauaugcaaa guuuuaucau auccacaagu uaacauuguc     3300

aagaaagucg aaagucaaac uggaggauuc ucaaaagaau caauacuccc gaaaggagau     3360

uccgacaaac uaaucccucg aaaaacuaag aaaguuuacu gggauaccaa gaaauacggg     3420

ggcuucgacu cuccaauaau cgcauauucg guguucguag ucgcggaugu cgaaaaggga     3480

aaagccaaga aacuaaagaa agugaaggaa cucguaggaa uauccauaau ggaaagaccg     3540

ucauuugaga aaaauccaau agucuuuuua gagaagaaag guuauagaaa cguacaagaa     3600

gauacauuaa uaaaacugcc caaauauagc cuauuugaau uugaaggcgg uagacggaga     3660

uuauuagccu cagcgaguga acuacaaaaa gggaaugaag uaguauugag ugauagacuc     3720

gucgaacuau uauaucaugc acauagagug aacaguuuua acaguaccga acaucuuaaa     3780

uacguaagug aacauaagaa agaauuuggu gaaguacuau caugugucga gaacuuugca     3840

aauucauaug uggauguaga gaaaaauuua gauaaaauca gagcaguaau aaauaaaaua     3900

gauaccuuuu caauagaaga aaucagcacc uccuuuguga auuuacuaac acuaaccgca     3960

uuaggagcac cagcagacuu uaauuucuua ggcaagaaaa uuccacguaa aagauacaca     4020

aguacaaaag agugcuuaaa cgcaacacua auacaucaau caauaaccgg ucuuuaugaa     4080

acaagaaucg aucucucaaa acuaggggaa gacaaauga                            4119


<210>  5478
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5478
augacaaaga aaaauuacuc aauaggccuc gauauaggua caaacucagu uggaugggca       60

gucauaacag augacuauaa aguaccagca aagaaaauga aaguacuggg uaacacugau      120

aagaaauaca uaaagaaaaa ccugcuagga gcacuucuau ucgacagugg ugaaaccgcu      180

gaagcuacca gauuaaaacg uaccgcuaga agacgcuaca cucgcagaaa gaauagacua      240

agauauuuac aagaaauauu ugcaaaugaa auagcuaaag uagacgaaag uuucuuccaa      300

cgacuagacg aaucauucuu aacugacgau gacaaaaccu uugauucuca uccgauauuu      360

gggaauaaag cugaagaaga cgcauaccac caaaaauuuc caacgauaua ccaccuaaga      420

aaacaucuag cggacuccuc agaaaaagcu gaccuaaggu uaguauaccu cgcauuagca      480

cacaugauua aauuuagagg ccacuuccuc auagaaggag aauuaaaugc agaaaacaca      540

gaugugcaga aaauauucgc ggacuucgug ggaguauaua auagaacauu cgacgauagu      600

caccucuccg aaauaacggu agacgucgcu aguauacuca cagaaaagau aucgaaaagc      660

agaagacuag aaaaccuaau caaauacuac ccuacggaga agaaaaauac cuuauuugga      720

aaccuaaucg cacuggcucu gggucuacaa ccaaauuuca aaacaaacuu caaacucucg      780

gaagacgcaa aacuucaauu uucgaaagau accuaugaag aagaccuuga agaauuacua      840

ggaaaaauug gugacgacua cgcggacuug uuuaccagug ccaaaaaucu guacgaugca      900

auacuauuau cagggauacu caccguggau gacaacagca caaaagcacc acuaucagca      960

ucaaugauca aaagauaugu cgaacaucac gaagacuuag aaaaauuaaa agaguucauu     1020

aaagcuaaca aaucggaacu uuaccaugau aucuucaaag auaaaaacaa aaacggauac     1080

gcaggauaua uagaaaaugg aguaaaacaa gaugaauuuu acaaauaccu caaaaauaua     1140

cucuccaaaa uaaaaaucga ugguagcgau uauuuccuag acaaaaucga acgagaagau     1200

uuccuuagaa aacaaagaac uuucgacaac ggauccauac cccaccaaau acauuuacaa     1260

gaaaugcacg caaucuuaag gagacaaggg gauuacuacc cguuccuuaa agaaaaacag     1320

gaccgaauug aaaagauauu aacauuccgc auaccauauu acguuggacc acucguaaga     1380

aaagacagcc guuucgcaug ggcugaauac agaucggacg agaaaauaac uccauggaac     1440

uuugacaaag ugauugauaa agagaaaagu gcggagaaau ucauaaccag gaugacacuu     1500

aacgauuuau aucugcccga agagaaagua uuaccuaaac acucacaugu guacgaaaca     1560

uacgcuguau acaaugaacu aaccaaaaua aaauauguaa augaacaagg gaaagaaucc     1620

uucuucgaca gcaauaugaa acaagaaaua uuugaucaug uuuuuaaaga aaacaggaaa     1680

gucacuaagg agaaauuauu aaauuaucuc aacaaagaau uuccggaaua cagaauaaaa     1740

gaucuuauug gccuugauaa ggaaaacaaa uccuucaacg ccagcuuagg caccuaucac     1800

gaucugaaga aaauauuaga caaagcauuc uuagacgaca aaguaaauga agaagucaua     1860

gaagacauua uuaaaacccu aacccucuuc gaagacaaag auaugaucca ugaaagacug     1920

cagaaguauu cagacauuuu uacugcuaau caacuaaaga aacuagaaag aagacauuac     1980

acuggauggg gaagacucuc guacaaacua auuaacggaa uacgaaauaa agaaaacaau     2040

aaaaccauuc uugacuaccu cauagacgac ggauccgcca acagaaauuu uaugcaacua     2100

auuaaugaug acacucuacc auucaaacaa auuauacaaa aaucacaagu aguaggagau     2160

guagaugaca ucgaagcagu aguacaugau cuaccugggu cucccgcuau caagaaaggu     2220

auacuccaau caguuaaaau agucgaugaa cucguaaaag ucaugggagg aaacccagac     2280

aauauaguaa uugaaauggc acgugaaaac caaacaacua acagaggacg aucacaaucu     2340

caacagagau uaaagaaacu acaaaauuca cuuaaagaau uaggaucuaa caucuuaaau     2400

gaagaaaaac cgaguuacau ugaagauaaa guagaaaauu cacacuuaca aaaugaccaa     2460

uuguuuuuau auuacaucca aaacggaaaa gacauguaua caggagauga auuagauauc     2520

gaucaucuau cugacuauga cauagaccac aucaucccac aagcauucau aaaagaugau     2580

agcauagaua auagaguacu aacaucuuca gccaaaaaua gaggaaaauc ugacgauguc     2640

cccaguuuag auauagucag agcaagaaaa gcagaauggg uacgacucua caaaucaggc     2700

uuaauaucca agcgaaaauu cgauaauuua acaaaagcug aaagaggagg auuaacagaa     2760

gccgauaaag cggguuucau aaaaagacag cuaguagaaa caagacaaau uacaaaacau     2820

guugcucaaa uucuagaugc aagauuuaac acagagcaug acgaaaacga caaaguaauu     2880

agggacguua aagucauaac ccuaaaaucc aacuuaguuu cgcaauuccg aaaagacuuc     2940

gaauuuuaua aagucaggga aauuaaugac uaccaucacg cucacgacgc auacuuaaau     3000

gccguugucg ggacagcauu acuuaagaag uacccaaaau uagcaucaga guucguauac     3060

ggagaauaca agaaauauga caucagaaaa uucaucacaa acagcucaga caaagccacc     3120

gccaaauauu ucuuuuacuc aaacuuaaug aacuucuuua agacaaaagu uaaauacgca     3180

gacgguacag uuuucgagag gccuauaauc gaaacaaacg cagacgguga aauugcaugg     3240

aacaaacaaa ucgacuuuga aaaaguuaga aaaguacuau cauaccccca agugaauaua     3300

gucaagaaag uugaaacaca aacgggaggu uuuucuaaag aauccauauu accaaaaggc     3360

gacucagaca aauugauacc cagaaaaacu aagaaaguau auugggauac gaagaaauac     3420

ggagguuucg auuccccaac uguugccuau agcguauucg ucguagcaga uguagaaaaa     3480

ggcaaagcca agaaacuaaa aacaguuaaa gaacucgucg guauaucuau uauggaaaga     3540

uccuuuuucg aggaaaaccc gguugaauuu uuagaaaaca aaggauauca uaauauaaga     3600

gaagacaaac uaauuaaacu accaaaauau agcuuauuug aauuugaagg gggccgaaga     3660

agauuauuag caagcgcauc agaacuacaa aaaggaaacg aaaugguucu accagguuau     3720

cuugucgaac uuuuauauca cgcacaccgg gcugacaauu uuaauucuac cgaauaccua     3780

aauuacguau cagaacacaa gaaagaguuc gaaaaaguac uuaguugcgu ggaggauuuu     3840

gcaaaucuau auguagaugu agagaaaaau cuaucaaaaa uuagggcugu ggcagauagc     3900

auggacaacu uuagcauaga agaaauauca aacucuuuca uuaauuuacu aacauuaaca     3960

gccuuaggag caccagcaga cuuuaacuuc cuaggagaga aaauaccgcg caagcguuau     4020

acaucuacaa aagaaugucu gaaugcaacc uuaauccacc aaucaauaac cgguuuauau     4080

gaaacacgga uagauuuguc caaacuagga gaagaauga                            4119


<210>  5479
<211>  4119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5479
augaacagug auaaaaggga agauuacuac guuggacugg auaugggcac uggaagucuc       60

ggaggugcug uuacagauac ccaguaucac cuacuaaaag uaaaaggaaa agauuucugg      120

uucguacgug aauacgagac agcaaaauca caauuagaua gaagaacaca ucggauagca      180

aaaagacguu uacaacggca caaagcacga auaggcuuaa ucagaucuua uuuugcagaa      240

gacauacuca agcaugaccc acuauucuac aucagacagg auaauuccaa auacuaccau      300

gaagauaaag acguacgguu aaccaccaaa gauucaauau uugcugaucc aggauacgga      360

gacaaggaau aucuagauga uaaagaauac ccaacaauuu uucaccuacg acaagcauug      420

uuaagagauc agauaaaaga cgaagaacgu uauagucguu ucuuauaucu ugcaaucauc      480

aauauguuug agcauagagg ucauuucuua cucaauacag aaucaagcga ucuuaacgcc      540

gacaugauaa aagacgucgg agaacucguc augaacgcua uauguggagu uagugaauac      600

ggaucaguaa ccuacaaaga aauuauagaa auuuuagaag acaaaaauau aucacggacu      660

gcuaaaaaag aucaaauagc uucaaaaaua gacaucaaaa aaucagaaaa agcucaaaug      720

gaaaggauca aaugcuuaug ugguauggaa gcagaugcaa gaguaauguu cgaaauagaa      780

gcggacgaaa agauuaaaau agcauuucaa caagcggcau uugaagauga aaaagaagag      840

auagaagcag ugauaggaga agacagauau uuaauaaucg aaaacaugaa acaacuauau      900

gauuauagcc aacuccaaag cguauuaaau ggauuugauu aucuaucaga cgcuagagug      960

caaauguaug aaaaacauaa agaagaccuu caccuauuaa aaaaagugua uagaaagaau     1020

cucgacagag aaauauacga cagaauguuu aggaguggcg aagaaggauc uuauugcgcg     1080

uacucuaaua guacaaauag cguagauagc cucgcugaag cuaaaaaaua cagacgaaac     1140

augaaaaaaa ggagucaaaa ggacuuauau gacagaauaa aaaaagaccu aaaggaagua     1200

caugaugcag augcugaugc aauacguaaa gaaauggaau uggaacgguu cuuacccaaa     1260

caaaucacag gggccaacgg uauuauuccg aaccagguac auagaaaaga auuaaaaaaa     1320

auacucgaga augcagagca ucaucuagac uuccuaaaag agaaagacga aaguggcuau     1380

acaguaucug aaagaauacu aauguuauuc ucaagcaaca uaccuuacua ugucggucca     1440

gugggcagug gaagcaagac cggcugggca aagauucaag aauccgguca aguauuacca     1500

uggaauaugg agcaaaaaau agauauagaa caaaccagug aaagauucau cacgaaucua     1560

aucagggagu gcacauaccu gucaggagag aaaguauuac caaaacaaag ucuauuauau     1620

gaaucauacu gcguccuuaa cgagauaaau aaccuccgaa uacacggcaa acgaauaguu     1680

ccagaacuaa aacaagauau uuauaaggaa guauuucaag gauuuagacu ugguaaaaaa     1740

guaacaaaaa aaaauauaug uacauaccuu auaaacagag gauuaauaac ggaagaaagc     1800

gaaguaagcg gaauagacaa agaaauaaac gcauaccuuc caucauaugg aaaaauguau     1860

ggcguauucg gagaaagacu aagagaagag gcuguucaag agauagcuga aaaaauaaua     1920

uacuacggaa cgauauacgg cgaugcaaag ucuauguuca gaaaaaaauu aaaccaauau     1980

guaagugacg gauggaugac agaauaccaa gcaaaaagaa uaaguggaua uaaauucaag     2040

gacugggcca gauuaucaag aucuauguua ugccucagcg gaugcgacaa gucaacuggg     2100

gaaaaaaucu ccuuaauaag agccaugugg gaauauaacc uaaacuuuau ggaacuaaua     2160

uacagggaag auuucacuuu ccaaaaggaa cuagaagaga gaaggaaagc auuaggcaag     2220

acguuacaag aguuccaauu cgaggaccua gaugaauauu auuacucagc accaguuaag     2280

aggaugauuu ggcaagcagu ucaagcauua aaagaaauaa cccaaauaau gggacauaau     2340

ccaaaacgua uauuuauaga aaugacaaga acggaagaag aaaaaggcga gcaaggaaga     2400

aaaaacagcc gagaaaaaag acuacuaaaa auguacgaaa gcauucgaga ugagagaaac     2460

ugggcagcag aaauaaaaaa ugcuaacgcu ucagguaaac uaaauagcaa aaagcucuau     2520

uuauacuauu uacaaauggg acgagauguu uacacuggag aacccauaga uauagaagac     2580

cuauucgacg acucaagaua cgacaucgac cacauauauc cccgaaguuu aacaaacgau     2640

aacaauauag aaaauaaucu cgugcuagua ucaaaaacaa uaaaucaaaa ugaaaaaaaa     2700

aacgauuauc ccguaccaga aaaaauuaga cgcaauaaaa aggugagaaa ucuuuggcau     2760

acacuacaua agaugggauu uaugaaugau gaaaaauaca aucgauuaac auccacugaa     2820

agacuauccg aggaccagcu cgcaggauuc auagcaaggc aacuaguuga aaccgcacaa     2880

ggagcaaaag gaauagccga auuaaugaag gcaaugcuac caggcacuga cauaguauac     2940

gcuaaagcua gaaauguauc agauuucaga aaagaauaca aaaugcuaaa aagcagguua     3000

guaaacgacu aucaccaugc acaggaugcu uaccuaaaca uagugguagg uaauguauac     3060

uuuaugaaau uuacaagaaa cccaaugaau uuuguaaaaa cggaagcaag acgggacgga     3120

cgaaauuaug acuacaaccu auauaaaaug uauaguaaag auaucauaag aaacggcgaa     3180

aaagcaugga uugcaacaag cgaacaagga cccgguacca ucagacuagu aaaagaaacu     3240

augggacgca auacaccuau aauuacacgc caagcguucg aacaaagagg agaauuauuu     3300

aauuugcaac cuguagguaa auauagugcc aaaaaagaua auuacgugcc gcucaaaaua     3360

aacgaugaaa aaaugcaaga cguaagcaaa uaugggggau acacaucucu aaauccaagu     3420

uauuuuauau uuauugaaca cgguccagaa aaaaaaagaa aaaaaugcuu cgaaguuaua     3480

cauucguauu acgcugcuca aauaaaaaca gaaaaagauu uaauagauuu ucucuuacag     3540

aagggcuaua aaaauccaag aguaauaaau gcuagaauua aaaaaaacgc acucauaaaa     3600

uacaacggau auuucuuaua cauuauagga auggaugcaa gaaaaaacau agaguucagc     3660

aaugcgacag caaugugucu gaaaaacaaa uauauucaau auguaugcaa auuggaaaaa     3720

augaacaaag caauucuauu gucggaaaaa cagaaaacua acuuacacug ggacgagaaa     3780

aucacaugug aaucaaacuu agaguuauau agggaauuaa cagaaaagca ucuacacucg     3840

aucuaccaaa gacaccccag aaguauagga aaaugcuuag cggacggaga aggugcauuu     3900

aaacuacuug auauagagga acaaguaaaa auaauaugcg auauaguaca auacacuucc     3960

uuucaacgcg gaguauuuag cuuaaaaguu cuaggaggac cgaaagaagu gggccgaaua     4020

agaauaagug guaauaugac cgaagcgaaa gaaugcaagu ugguaaauua cucaauaaca     4080

ggcauguaua aaacagaaau ggaucuucua aaaaaauga                            4119


<210>  5480
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5480
auggaaaaaa cauacucuau cggacuggau auaggcacua auuccguugg cuggucgguc       60

auaaccgaug acuacaaagu gcccgccaaa aaaaugaaag uacuaggcaa uaccgauaaa      120

aaauauauca aaaaaaacuu acucggugcg cuccuuuuug acaguggaga aacugcagaa      180

guaacaagau uaaaacguac agcaagacga cgcuacacac gccgcaaaaa ucgacuuaga      240

uauuuacaag aaaucuucgc aaaagagaug gccaaaguag acgaauccuu uuuccaaaga      300

uuagaagaga guuuccucac ugacgaugau aaaacauucg auucucaucc uauauucgga      360

aacaaagcag aggaagacgc cuaucaccaa gaauuuccaa caauauacca ccugagaaaa      420

caccucgcug auucgucaga aaaagcugac uuaagauugg ucuauuuagc acucgcucac      480

augauuaaau ucagggguca cuuccuaauc gaaggugaac uaaaugcaga aaauaccgac      540

auacaaaagc ucuucaacga cuuuguagaa acuuacgaua aaauagcgga cgaaucccau      600

cuuagcgaag uugaugccuc aucuauacua acaaacaaga uaucuaaauc gcgaaggcuc      660

gaaaacuuga ucaaacaaua cccaaccgaa aaaaaaaaua cccuauuugg aaaucuuguu      720

gcauuagugu uaggacuaca acccaauuuu aaaaccaauu ucaaguuauc agaagacgca      780

aaacuccagu uuucuaaaga uacauaugac gaagaucuag aagaacuucu ggggaaaaua      840

ggggaugauu augcagauuu guucaccgca gcuaaaaacu uauaugacgc aauauuacug      900

ucagguauac ugacuguaga ugacaauucu acuaaagcac cccuaagcgc cucaaugauc      960

aaaagauacg aagaacacca cgaagaucug gaaaaacuca aaaaauucau aaaaguaaac     1020

aauuucgaca aauaucacga aauauucaaa gauaaaagca aaaauggaua cgcuggcuau     1080

auagagaaug gcguaaaaca agauauuuuu uacaaacacu uaaaaucaau cauaagugaa     1140

aaaaauggug gucaauacuu cuuagacaaa auugaacgag aagacuuccu cagaaaacaa     1200

agaacauuug acaacggauc cauuccccac caaauccacc uccaagaaau gagggcgaua     1260

uuaagaagac agggggaaua cuauccguuu uuaaaagaaa aucaagccaa aaucgaaaaa     1320

auucuaacau ucagaauucc uuauuacgua ggaccucucg cgagaaaaga caguagauuc     1380

gcaugggcaa acuaccacuc cgacgaacca auaaccccau ggaauuuuga cgaaguagua     1440

gauaaagaaa aaucagccga gaaauuuaua acaagaauga cacuaaauga ucucuauuua     1500

ccugaagaaa aaguacuacc caaacauucu cacguuuacg aaaccuuuac agucuacaac     1560

gaauuaacua aaaucaaaua uguaaaugaa caaggagagu cauuuuuuuu ugacgcaaac     1620

augaaacagg aaauauucga ucacguuuuu aaagaaaauc guaaaguaac aaaagcaaaa     1680

uuacucucau aucuaaauaa ugaauucgaa gaauuuagga uaaacgaucu aauaggacuc     1740

gacaaagaca gcaaaucauu caacgcuagc cuagguacgu aucaugaccu uaaaaaaauc     1800

cuagacaaau cauuccuuga cgauaaaacg aacgagcaga uaauagaaga cauaguccug     1860

acauuaacac uauucgaaga uagagacaug auccacgaac guuuacaaaa auauucagac     1920

uuuuuuacua gccaacaauu aaaaaaacua gagaggagac acuauaccgg augggggaga     1980

cuuucauaca aauuaauaaa cggaauaagg aacaaagaaa auaacaagac gauauuagau     2040

uuccuaauag augaugguca ugcuaauaga aauuuuaugc aacuaaucaa ugaugaaagc     2100

cuaucauuca aaacaauaau ccaagaggcu caaguagucg gcgacguaga ugauauagaa     2160

gcuguuguac acgaucuccc agggagccca gcuauaaaaa agggaauauu acaaucugua     2220

aaaauaguag acgaacuagu aaaagucaug ggcgacaauc cggauaacau aguuauagaa     2280

auggccagag aaaaccaaac cacuaaccga gagaggaguc aaucccaaca aagacuaaaa     2340

aaacugcaaa auucucuuaa agaauugggc ucuaacauuc uaaaugagga aaaaccauca     2400

uacauagaag acaaagucga aaacagucac cuacaaaacg accaacuguu ccuguacuau     2460

auacaaaaug gaaaagauau guauacuggu gaugaauuag acauagauca cuuaagcgau     2520

uacgauauag aucauauaau accgcaagca uuuauaaagg acaauagcau cgauaacaga     2580

guacuuacua gcagcgcaaa aaaucgagga aaaagcgaug acguuccuuc acuagauauu     2640

gucagggcaa ggaaagcaga auggguacga uuauauaaaa gcgguuuaau aucaaaaagg     2700

aaauuugaua acuuaacgaa agcggaaaga ggaggccuaa cagaagcaga caaagcagga     2760

uucauaaaaa gacaauuggu ggaaacacga caaauaacaa aacauguagc ccaaauacuc     2820

gaugcuaggu ucaacacgaa acgagacgaa aacgauaaag uuauuagaga cguuaagguu     2880

auaacacuca aaaguaaccu cguaucacag uuuagaaagg aauucaaauu cuacaaagua     2940

agggaaauaa acgauuauca ccaugcccau gaugcauacc ucaacgcagu aguugguaca     3000

gcuuuacuaa aaaaauaucc caaacuaaca ccagaauucg uuuauggaga auauaaaaaa     3060

uacgauguaa gaaaacuuau agcgaaaagc uccgacgacu auucagagau ggguaaagca     3120

acugccaaau acuucuucua cucaaaucuu augaauuucu uuaaaaccga aguuaaauau     3180

gcugacgguc gaguuuuuga aaggccagac aucgaaacaa augcagaugg agaaguugua     3240

uggaacaaac aaaaagauuu ugacauagua cgaaaaguac ucagcuaucc ucaagucaau     3300

auagucaaaa aaguagaaga acaaacagua ggccaaaaug guggauuauu ugacaacaau     3360

auuuuagcua aaccugaaaa agaugauaaa aaaaaacuuu uccccaucaa aaagagcuua     3420

aguacaaccu cuuauggugg auacgcccga gcaacaauag cguacucuau ucucauagua     3480

agcgaguucg gcaaaaauaa agaacuuaaa uuauuaggcc uaccucuauu agauaaagua     3540

aaauaugaaa aaaacccuau agcuuaccua aauaguauag guaucaucaa cguuaaauca     3600

auauucaaau uaccaaagua uugucuauuc gaauuucaag aaaaaaacac aaauuauaga     3660

agauauaugg ucucuaacca ggaacuaaaa agagccaacc aacuauuccu aacaugcaaa     3720

gaaaccgaau uaguauacaa auauauaaaa uccgacaaug acaacaccca ccucgucggu     3780

aacgaaaaga caaucuuauc uaucuggaau aaauuaguaa uauuuaugga acggacuaaa     3840

uuaauagaua aaaaacuauu ugcaagccua aaacaacuaa aagaugaagu cgaucucaua     3900

aacauacuug acaugacugc aaaaagccua gacuucacaa aaauaucagc cggccaaaaa     3960

aaaauaacaa uaagcgauuc auuaagucua cccauuaaaa gauggcaguu uaguucagac     4020

gaaaaaaugc uaaaacaauu aaaaacagca accuuaaucc aucagucagu aaccggacua     4080

uacgaaacua gaauugauuu aucuaaacuc ggugaagaau ga                        4122


<210>  5481
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5481
auggagaaaa cauauuccau aggucucgau auaggaacaa auagcguagg auggagugua       60

auaacagacg auuacaaagu gccagccaag aaaaugaaag uacuaggaaa uaccgauaag      120

aaauauauca agaaaaaccu acugggcgcu uuacuauuug acucagguga aacagcagaa      180

gucacuagau uaaaacgcac agcacgaaga agguacacgc gaaggaaaaa ccgccuaaga      240

uaucuacaag aaauuuuugc aaaagaaaug gcaaaaguag augaaucauu cuuucaacgu      300

uuagaagaau cauuucuaac agaugacgac aaaacuuucg auucacaccc aauauucggg      360

aacaaagcug aggaagacgc guaccaucaa gaauuuccga ccaucuacca cuuacgcaaa      420

caccuagcgg acucaucuga aaaagcagac cuaagauugg uauaccucgc auuagcucac      480

augauaaaau ucagagggca cuuccugaua gaaggagaau uaaacgcaga aaacaccgau      540

auacaaaaau uauuuaacga cuuuguggaa acauacgaca aaauagcaga ugaaagccau      600

uuaucagaag ucgaugcauc aucuauccuc acaaauaaaa uauccaaauc aagacgacuc      660

gaaaaccuaa uuaaacaaua uccaacagag aagaaaaaua cacucuucgg gaaccuagua      720

gcccuaguac ucgggcugca acccaauuuu aaaacgaacu uuaaauuguc agaagaugca      780

aaacuucaau uuucaaaaga uacauacgau gaagaccuag aagaacuuuu aggcaaaaua      840

ggggacgacu acgcagaccu auuuacagcg gccaaaaacu uauacgacgc aauccuccuc      900

uccgguaucu uaaccguaga ugauaauucc acuaaagcgc cccuuucagc aucaaugauu      960

aaaagauacg aagaacacca ugaagaucua gaaaaacuca agaaauucau uaaaguaaac     1020

aacuucgaua aauaucauga aauuuuuaaa gauaaaucga aaaacggcua cgcaggcuau     1080

auagaaaaug gaguaaaaca agauaucuuc uacaaacauc ucaaaucuau aauaucugag     1140

aaaaacggcg gacaauauuu ccucgacaaa auugaacgcg aagacuucuu acggaaacaa     1200

cggacauuug acaauggcuc aauuccgcau caaauacacc uucaggaaau gagagcaauu     1260

cuacgucgac aaggggaaua uuauccauuc cugaaagaaa aucaaucaaa aauagagaaa     1320

auacuaacgu uuaggauucc cuauuacgua ggaccccuag caaggaaaaa uucaagauuc     1380

gcaugggcua aauaccacuc agaugaacca auaacccccu ggaacuucga ugaaguagua     1440

gauaaagaaa aaucagcuga aaaauucauu acaagaauga cccuuaacga ucucuaccua     1500

ccagaagaaa aaguccuacc caaacauucc cacguuuacg aaacauacgc cguauacaau     1560

gaauugacaa aaauuaaaua cguaaaugaa caagguaaag aaucauucuu cgauucaaac     1620

augaaacaag aaauauuuga ucacguguuc aaagaaaacc gaaaaguuac uaaagaaaaa     1680

uuacuaaauu accuaaauaa agaguucccc gaauacagaa uuaaagacuu aaucggacua     1740

gauaaagaaa acaaaucuuu caaugcuuca uuagggaccu accaugaucu caagaaaaua     1800

cuagauaaau cuuuccugga cgacaaagua aacgaagaag uuauagaaga uauaauaaaa     1860

acacuaaccc uuuuugaaga caaagauaug auacaugaac gcuuacagaa auauagcgac     1920

auuuucaccg caaaccaacu aaagaaacua gaaagacgcc auuauacagg guggggaaga     1980

uuaaguuaua aacuaauaaa cggcauucga aacaaagaaa auaauaaaac aauccucgac     2040

uaucuaauag acgacgguag ugcuaacaga aacuucaugc aauuaauaaa cgaugauaca     2100

cuaccauuca aacagauuau ccaaaaaucc caaguaguug gagacguaga ugauauagaa     2160

gcaguagucc augaccuacc cggcagucca gcaaucaaga aagggauauu acaauccguu     2220

aaaaucgugg acgaauuagu aaaaguuaug ggaggaaauc cagauaacau agugauugaa     2280

auggcacgag aaaaucaaac cacuaaucga gguaggucgc aaucacaaca aaggcucaag     2340

aaauuacaaa gcucauuaaa agaacuaggu aguaacauca uaaacgaaga gaaaccguca     2400

uacauugaag auaaagucga aaacagccac uuacaaaacg accaacuauu uuuauauuac     2460

auacaaaaug gaaaagacau guauaccgga gacgaacuag auauagacca uuuaagcgau     2520

uaugauauag aucacauaau accgcaagcc uuuauaaaag augauaguau agacaaccgg     2580

guacuaacaa guuccgccaa aaauaggggu aaaucagaug acguaccuag ccucgacaua     2640

guaagagcac gcaaagcaga auggguuaga cucuacaaaa gcgggcucau cucaaaacga     2700

aaauucgaua accuaacuaa agccgaacga ggaggacuaa cagaagccga uaaagccgga     2760

uuuauaaaaa gacagcuagu agagacgcgc caaauaacua aacauguagc ccaaauucuu     2820

gacgcgcguu uuaauacuga acaugaugaa aacgacaaag uaauaagaga cguaaaaguu     2880

auaacccuaa aauccaaucu aguaucgcaa uuccgaaaag acuucgaauu uuacaaagua     2940

cgcgaaauaa augacuacca ucaugcacau gaugccuauc uaaaugcagu agucggaacg     3000

gcauuacuaa agaaauaccc aaaauuggca ccagaauuug uauauggaga auauaagaaa     3060

uacgacguga ggaaacuaau ugcaaaaucu ucagacgacc acucagaaau gggcaaagcu     3120

acagcaaaau acuucuuuua cagcaacuua augaacuucu ucaaaaccaa aguaaaauac     3180

gcagacggaa gugucuacga acgaccaaua aucgaaacaa acgcggaugg cgaaguagug     3240

uggaacaaac aaaaagauuu ugaaaucaua cgaaaagucu uaucauaccc ucaggugaau     3300

auagucaaga aaguagaaga acaaacaguc ggccaaaacg guggacuuuu ugacaacaau     3360

auacucgcaa aacccgaaaa agacgauaag aagaaacuuu uuccgauuaa gaaauccuua     3420

aguacaacuu cauacggcgg auaugcaaga gcaaccaucg cauauucgau uuugauaguu     3480

ucugaauuug guaaaaauaa agaguuaaaa uuacuaggac uuccccuuuu agacaaaguu     3540

aaauacgaga aaaauccaau agccuaccua aacucaauag gcauaauuaa uguaaaauca     3600

auauuuaagu uaccaaaaua uugucuauuu gaguuccaag agaaaaacac aaacuacaga     3660

cguuacaugg uaucaaauca agaauugaaa agagcuaauc aacuuuucuu gacuuguaaa     3720

gaaacagaac uuguguacaa auauauaaaa agugauaaug auaauacuca cuuggucgga     3780

aaugagaaaa cuauccuuag uaucuggaau aagcuaguua uauucaugga aagaacuaaa     3840

cuuauagaua agaaacuauu ugcaagucuc aagcaacuaa aagaugaagu caaccuaaua     3900

aacauccuug acauagcauc uaaauccauu gacuucacaa aaauuagcgc aggucaaaag     3960

aaaauaguac uuaaugauuc ucucucuuua ccaauaaaac gauggcaguu caaaucagau     4020

gagaaaaugc ugaaacaauu aaaugaugcg accuugauuc accaaagcau uacaggacuu     4080

uaugaaacca gaauagaccu aagcaagcug ggagaagaau ga                        4122


<210>  5482
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5482
augaauuuaa gacgagcuag agauuacagc auaggacuag acuuagguac aggaagugug       60

gguugggcug ucacagacgc agaaggaaau cuauacaguu ucaaaggaag accggcaaug      120

ggcaguagaa cauuuccaaa ugcggaaccc gcaucaguag ccagaauacc gaggggucaa      180

aggcgaagau acgaacguag aagacaaaga auaaaacuac uucaacaauu cuuuaugagu      240

gacauggaaa gagucgaucc cgacuucuuu acgagauuaa aucaaucaac acuauuagca      300

gaagauaaag augagagaau agcaaguugc gcauggccua uauuuaauuc auccgacuuu      360

acagaaaaag auuacuacaa gaaauuuccg acuauauauc auuuacgcgc uuuucuuugu      420

gaaagcgacg aaaaaggcga ucuacgacuc guguauuuag cccugcacaa uauaguaaaa      480

cauaggggaa acuuuuugua ccaagauaau ccugcauuga gugcaucuga ugcuucaguc      540

gaugaagcgg uuagacaauu aguagaugca cuagcugaau ggugucagaa uaaagaaaua      600

gagcuuucuu gugaugcagg gagaauuaua gaaguguuug gugaugcauc gcucagaaga      660

acugauaaaa gagaucaauu aaaaggauua uuaggauuac cugcagaucu agcgaaaacu      720

augggcaaag agcuugcauc ggccguauua gguuaugcag caaacuuuuc uaaaaucuuu      780

cuaauaucag augcagaaca uaaguuuuca uuagggaacg acgaaaaggu cgaggaauuu      840

gaaacaaucu gcccaaauga auguaccagc cucuucagcg cacuacguac agcguacuca      900

gcauauuuau uaucuggcau ucuaagagau gcuaauggaa aaacauuaag uuucuguaag      960

aagaaagaau acgaucgaua ucacgaugau uuagcauuac uuaaagcauu aguuagagaa     1020

uacgcgcccg aaaguuauca aucauucuuu ggaggggaac gguuaguagu aaacggcaga     1080

augacaaaac gguaugaucc aacaaaagcu aaaggguaua ccaaauacga ucuuguucau     1140

agugcuggua guuacgacga guuagcaaaa gauguaaaga aacuccuugg gaguuuagca     1200

gcugaugauu caagauauca acgaaugaug caagcauuug augaagaacg guuucuuaga     1260

aggcuuaaaa cgucugauaa ugguucgauu ccauaucaac uucaccuaga agaacuaaga     1320

gcaauaauag caaggcaagg ugaauuuuau ccauucuuaa aagaggaaag uaaaaagaua     1380

gaaucccuag uaaguuucag gauaccuuau uauguagggc cauuaacgac uaaaaacgcc     1440

gcuguagaug uucacgguaa uaaacgauuu gccuggucgg agagaaagcc cggaauggag     1500

agcgcuaaag uaagaccaug gaauugggac gauguaaucg acagagguaa aucagccgaa     1560

aauuuuauag uaagaaugac aggaacaugc acuuaucucc aaaacgagaa agucuuacca     1620

aaaugcagcu uacuauauga ggaauuuugc guguuaaacg aauugaacgg agcccauugg     1680

uccgcugaug gagaccauga gugccccuuu gauacaagcg acagaagggg aauaguccag     1740

gauuuauuuu gugggaaaag aagagucuca uacaaacaag ucgaagacug gcuaagacag     1800

cacgguucgc ggagagcaag aguauccgga ggacaaggug cgagugguuu ugaaucaaaa     1860

augucuucgu auaucuucuu cucaaaagau guuuugcaug uagauagaau agauccugca     1920

gauuucccua ugauugaaga aauuauacuu uggaauacgu uauucgagga ccgagauaua     1980

uuaaaagaaa gaauagaaga agcguauggc gacagacuaa augcagaaca aauuaaaaca     2040

auaugcaaaa agagguucac aggauggggg cguuuaaguc gaagacuuuu aacaggaaua     2100

aaggcucaaa cagaccaagg ggauuuaagu auaauggaca uuuuaagaga aggagauccg     2160

aauaaugauc acagagguaa agcaauggug uucaugggaa uacuccauag cgaagauuua     2220

ggauuucaaa agaaaaucga ucagauuaau caagagaaag uuucugcagg uaaaguuagu     2280

uuagaggauu ugccugguag uccagcacuu agaagaagca uaaaccaagc aaugagaguu     2340

gucgacgaaa ucguuucaau ugcaaaaugc ccacccucuc auauauaucu agaagucaca     2400

agagaugaag auggagcaag gaaaggacga agaaguacga gaagauacga cgcuauaaaa     2460

gcugcauuag aaaaauuuaa agaagaguca cccacauggu ucgaccaaga cgucuuugga     2520

gaauuuagaa gaagcagagc agaagaucua gacgaaagau uaucgcucua cuuuaugcaa     2580

ggagggaaaa gucuauacuc agcuacacca cugaaauugg aacuauuaca cacuaaucaa     2640

uaucaaguug aucacauaau uccucaauca uauauaaaag augacagcuu ugaaaauaag     2700

gcuuugguac uagcuggaga aaaucaaaga aaaagcgaug cucuauuaau agacucgagu     2760

auacgaacug cgauggcccc auacuggcgu gcucuccauc aagccgguuu aaugggggau     2820

aagaaauuua auagcuugau gugcucgucu auaagugaca aaagaguaag aggauucaua     2880

gcaagacaac ugguagaaac aagccaaaua guuaaacuag caggaaugau guuacaaagu     2940

aauuacccag aagcagaugu gcaaccagua aaagccucgu uaggacacga ucucagagua     3000

caagcuggau uuauuaaaug cagagaagug aaugacuuuc accacgccca ugaugcccuc     3060

auugcaugug aaguugguag guauauaaau caaugucauc ccggcauuua ugauaauccu     3120

auaggauacg caagagcugu aagggaacuu guaagaaagc agaaugaaga aaugcguaaa     3180

uacggcagac uuccaggauc agcaggguuu auaguaggag guuucaugag aacccaugua     3240

gacguugaua caggagaagu cguuugggac gcugaagagg aacuaucccg aauaagagca     3300

uaccuugacu auucaaacgu auucgucaca cgaauguccg aagaaacauc aggugcguug     3360

uggaaugcua caaucuacag cccacacuac gggaaaacug caccaacauu acacauaaaa     3420

caaggauuag auguacaaag auacgguggg uauuccucag cuacauucgc uuauuuuacu     3480

uuauauguag cagaaaaagg aaagaaaaga aaagcggaac uauuaggaau accaauauca     3540

guagcccgga aaauagaagg agaaccaagu aaauuggaug cauucguagc ucaagaugcc     3600

gaagcgaaga aacuaaaaca cccacuuaua auaagagcac gaauauuaaa auacgcaaga     3660

uuugaaaugg aggguaauca auuuuauuuu gcagcacaag augcaguuua cucagcaagg     3720

caguuaguau uaaaagguga ucuauacuca guagccgcaa aagccuguga uaaacacuug     3780

aaugacagcu guaucaguaa ugaagacuua auucgguuau augaugaauu gguacauaag     3840

ggggauuuau uaugcaauaa auuuggcaau guuacggaug uucuccgagc aggcagagaa     3900

agauaugcag ggcuaaguau aagugagaaa cgacagcuag ucuguaaugu ucuggccuau     3960

uacagauccg ccacggcaag aguugacaua ucauuaguag guggacccaa acaugcaggu     4020

aauuuaggga ucgccuuugu auuaaacaau ccagaagcaa uagucuucgu agaucaaagu     4080

guaacaggga uguuugagaa aagaaccaga guagaacuau ga                        4122


<210>  5483
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5483
augaagaaaa uacuuggucu cgaccuagga acaaacucga uaggaugggc uuuaguaaac       60

gaagcggaca acgaaagaga aaaauccagc auaauaaaaa uaggagucag aguaaacccg      120

cuaacagucg acgaacaaca aaauuucgaa aaaggaaaau ccauaacaac uaacgcagau      180

cguacucuua aaaggagcau gcgccguaau uuacaaagau ucaaacuacg aaaagaaaac      240

uugauagaaa uucucaagaa acaaaaauuu auaaaugaug aaacaauauu aagcgaaaau      300

ggaaacaaau ccacuuuuga aacauacaau cugagggcga aaagugcuac agaagaaaua      360

acacuagaac aauuugcacg uguacuacua augaucaaua agaaacgagg auacaaaagu      420

agcagaaaag caaaaucaca agacgaagga caauuaauag auggaaugga aauagccaag      480

aaacuauaua augacaaucu aacgccaggg caauucguuu uccaaauucu gaaagaaggu      540

aagaaauaca uacccgacuu cuacaggagc gaccuucaag ccgaauuuga caaaauaugg      600

ucauuucaaa aacaauuuaa ccccgauauc cuaacagaag aauuuaauaa acaaaucaua      660

ggaaaaggga aaguaaauac aacuaaaaua uuucuggcaa aauacgguau cuauacaaua      720

gacaauaaag gggcagacaa gaaaucucaa gcauacaacu ggagagucga agcccuaacc      780

aaccaauuga cgaaagaaga auuagcauuc guaauaucag accucaacgg aaucauaagc      840

aauagcuccg gauauuuggg auccauauca gacaggucca aagaacuaua uuuuaacaaa      900

caaacagucg gacaauaccu aauugcucaa uuaaaagaaa acccccauac ccaacucaaa      960

aaucaagugu ucuauagaca agacuaucuc gaugaguuca acacaauaug ggaaacccaa     1020

gcaaaauuuc auaaagaauu gaccaaugaa uuaaaagaag aaauacgaga uauuauaaua     1080

uucuaccaaa gaaaacugaa aucucaaaaa ggccuuauau cauucugcga guucgaauca     1140

aaacaaauag aaauuaauau agacggcaaa aagaaauuaa aaacagucgg aucuaaagua     1200

ugucccaaau caagcccccu cuuucaaguc uuuaaaauuu ggcaaauacu aaacaauaua     1260

caagugauag aaaaaucgac aggcaaaagu aaauuuuuag aacaagauga aaaagaaaua     1320

cucuucaaag aauuaaauuu caaagauaaa auaaguaaaa cagaugcccu caaaauucua     1380

uauaaaaauu auaaagaauu agauuucaau uuuaaagaaa auauagaagg aaaccguacc     1440

caagcaacau ucuuaaaagc auuucaauca auaauagaau uaucaggaca cgaaguaucu     1500

gauuuuagca aaauaaaaac agacgaaaua cuugaaauug uaacuccaau uuucgaaggg     1560

uucggauuca auacaaauuu auuacaauuu aauaguaacu uaaaaggaaa agauuucgaa     1620

aaacaagccg uuuaucaacu uuggcauuua uuauacucau ucgaaggaga caacucgaau     1680

acaggcaacg aaaaauuaau acagaaaaua agcgaacuau acgguuucga aagagaguac     1740

gcaauaauaa uugccaacau cuccuuucaa ccagacuacg gcuccuuauc gacuaaagcu     1800

auaagaaaaa uccuaccuca ucuaaaagaa ggaauagaau uuucauuagc augugaauac     1860

gccggcuacc gacauagcaa aaacucacua accaaagaag agaaaaacaa gaaaauauua     1920

aaagauaaaa ucgacauuuu accgaaaaau agucuacgca acccagucgu cgagaaaauu     1980

cuaaaccaaa ugauaaacgu agucaacgca acuaucgaaa cauauggcaa accugacgaa     2040

auacgaaucg aacuagcucg agaacucaag aaaucggcca aagagcgaga agaccuaacc     2100

aacgcuauua auaaaucaac cuuggaucau gaaaaauaca aagcacugcu acaaaaagag     2160

uucggacuau caaaugucuc cagaaacgau aucauaagau acaaauuaua ucuagaacuc     2220

gagcacaacg gcuuucacac auuauauuca aacacuuaca uagaaccaaa caaacuauuu     2280

ucaaaggagu ucgauauaga acacauaauu ccccaaagca gacuauucga cgacucguuc     2340

ucaaacaaaa caauagaauc ucguagcgcu aauaucgaaa aagguaauga aacagcauuc     2400

gacuacguaa gcaacaaaaa uggcgaagua ggacucaagg auuaucuuaa gaacauagaa     2460

gaccuauuua acaaugguaa aaucucaaaa acuaaauaca acaaauuaaa aaugaaaggu     2520

gacgaaauac cgucagacuu caucgcccga gaccuaagag acacucaaua cauaucaaag     2580

aaagccaaau cuauuuuaga agaucuagua aaagacguag ucucaacagg ugguuccauc     2640

acugauagac ugcgcgaaga uuggcaauua auagauauaa ugaaagaacu caacugggau     2700

aaauaugaca aauuaggucu aaccgaagua auagaaaaca acgauggaca caaaauaaac     2760

agaaucaaag auuggacaaa aagaaacgau caccgucauc augcaaugga cgcauugaca     2820

auagcauuca caaagagauc cuacauacaa uaccucaaca auuuaaacgc aagaucugac     2880

aaaucaggaa gcauuuacgg gauugagcaa aaagaauuau aucgcgauaa aaacaauaag     2940

cucagauuua aaccaccaau accacuagac gauuuuagag cagaggccaa aaagcaacug     3000

gaaaacaccc uaguuuccau aaaggcuaaa aacaaaguag cgacaaucaa cauuaacaau     3060

acuaagaaac aaggcggaga gaacaagaag aaacaacuaa ccccacgugg ccaacuacau     3120

cuagaaacca uauacggguc gauccaacaa uacgucacua aagaagaaaa aguaggagca     3180

aauuuuaaca aagaacuuau agaaaaagua gcaaacaaga aaugcagaga agcacugcuc     3240

aaacgcuuaa uagaguucga caacgaucca aagaaagcau ucaccaggaa aaacucacuu     3300

aauaaaaauc cauuauacau cgacacuaua aaaaccuaca aaguaccaga aaaagucaaa     3360

accguaacua cagaaacaau auacacgauc agaaaagaca uaaauccuga ccugaaaauc     3420

gauaaagucg uagauaagaa aaucaaaaac auccuagaac ugagauuaaa agaguucaau     3480

ggagacgcga aaaaggcuuu cuccaacuua gaugaaaaac caauaugguu gaacaaagaa     3540

aaaggaaucu ccaucaaaag aguaacaaua acugggauca guaaugcuau agcgcuucac     3600

gacaagaaag auaaggaagg aaacuuuaua cuagacaacc aaggcaagaa acaagccgua     3660

gauuuuguaa auacaggaaa uaaccaucau guagccaucu auaaggauga aaaaggaaau     3720

cuccaagaaa acgucauaac auucuacgaa gcagucgaac ggguuaauca ggguauccca     3780

auaauagaua aaacauacaa acaaaaugaa ggauggcagu uccuauuuag uaugaaacaa     3840

aaugaauacu ucguguuccc caaugagaaa acaggauuua acucgaauga aaucgaucua     3900

cucaauccag aaaauuacca ucuuauaucc ccaaaccucu ucagaguaca gaaaauagca     3960

acaaaagauu acuucuuucg ucaucaucuc gagacuaaug ucgagaacau aacaaccaca     4020

aaagacuuaa cguggaaacg ccuuggaaua aacggaauaa uaaacauacu uaaagucaga     4080

auaaaccaca uagggcaaau aguaucagua ggugaauacu ga                        4122


<210>  5484
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5484
augacaaaaa aaaacuacuc aaucggccuc gacauaggaa cuaauucagu aggcugggca       60

gucauuacag acgauuacaa aguaccagca aaaaaaauga aaguacuagg aaauacggac      120

aagaaauaca uaaaaaaaaa ucuacuugga gcauuacucu ucgacucugg cgaaacagca      180

gaagcaacca gauuaaaacg cacugcccga cgacgauaua cucgcagaaa aaaccgccua      240

agguacuuac aagagauauu ugccgaagaa augacaaaag uagacgaauc auucuuucaa      300

agacucgaug aaucauuucu acgaugggau gacgauaaua aaaaauuagg acgauaccca      360

auauucggaa acaaagcaga cgucguaaaa uaccaccaag aguuuccaac aauauaucau      420

cuacgaaaac acuuagcaga cuccagcgaa aaagcagacc ugcgucuugu auaucucgcu      480

cucgcacaca ugauaaaauu uagaggacac uuuuuaaucg aaggagaacu caaugcugaa      540

aacacagaug uccagaaaau auuugcugac uucguagggg uguaugaccg aacauucgau      600

gauucgcauu uauccgaaau cacaguagau gcagcuagua uacucacaga aaaaauaucu      660

aaaucacguc gacuagagaa ccuaauuaaa uauuauccua cagaaaaaaa aaauaccuua      720

uuugguaauu uaauugcgcu agcacuaggc cuacaaccua acuuuaaaau gaauuucaaa      780

uuguccgaag acgccaaacu acaauucagc aaagacucuu augaagaaga uuuaggcgag      840

cuguuaggua aaaucgguga ugauuaugca gaccucuuua ccagugcuaa aaaccucuac      900

gacgcaauac uauuaucugg aaucuuaaua guagaugaca auaguacuaa agcaccucua      960

agugccagua ugauaaaaag auauguagaa caccaagaag auuuagaaaa guuaaaagaa     1020

uucaucaaag cuaacaaaag cgaacuuuac caugacauau ucaaggauaa aaacaaaaau     1080

ggauaugcag guuacauaga aaauggagua aaacaagacg aguucuauaa auaucuaaag     1140

aacacgcugu cgaaaauugc agguuccgac uauuucuugg auaaaauaga aagagaagac     1200

uucuuacgaa aacaacgaac cuucgauaac ggauccauuc cacaucaaau ccaucugcaa     1260

gaaaugcacg caauauuaag aagacaagga gauuacuauc ccuucuugaa agaaaaucaa     1320

gacaggaucg aaaaaauauu aacauuucga auucccuauu auguuggacc cuuagcaaga     1380

aaggauucaa gauuuucuug ggcugaauac cacagcgaug aaaaaauaac accauggaac     1440

uuugacaaag ucauagauaa agaaaaaagu gcagaaaaau ucauaacaag aaugacgcuc     1500

aacgaccucu acuugccaga agaaaaagua uuaccuaaac acucccaugu auaugaaacg     1560

uaugccguau auaacgagcu aacaaaaaua aaguacguua acgaacaagg aaaagauucg     1620

uucuuugaca gcaauaugaa acaagaaaua uuugaucacg uguuuaaaga aaaucguaaa     1680

guuaccaagg aaaaacugcu aaacuauuua aacaaagaau ucccagagua uagaauaaaa     1740

gaccuuaucg ggcuugacaa agaaaacaag agcuucaaug caucacuagg cacauaccau     1800

gaucucaaaa aaauccuaga uaaagcauuc cuagacgaua aaguaaacga agaaguaaua     1860

gaggauauaa uaaagacguu aacccucuuu gaagauaaag auaugaucca cgaacgacuc     1920

caaaaauaua gcgacaucuu cacagccgac caauugaaaa aacuagaacg uagacauuac     1980

acaggauggg gcagauuauc cuauaaacua auaaauggga uaagaaacaa agaaaacaac     2040

aaaacaauac ucgacuauuu aauagacgau ggcucagcaa aucgcaauuu caugcaacuc     2100

auaaaugaug acacacugcc auucaaacaa aucauacaaa aaucccaagu aguaggagau     2160

guagacgaca uagaagcagu uguacaugac uugcccggau cucccgcuau aaagaaaggc     2220

auccuacaau ccgucaaaau aguagaugaa cuaguuaaag uaauggguga caauccagac     2280

aauauuguga uagaaauggc aagggaaaac caaacaacga auagagggag aagccaauca     2340

caacaacgau uaaaaaaacu gcaaaacagu cuaaaagaau uaggaucaaa uauuuuaaau     2400

gaagagaaac caaguuacau agaagacaaa gucgaaaaua gucauuuaca aaaugaccaa     2460

uuauuccugu acuacauaca aaauggaaaa gacauguaca ccggagauga auuagacaua     2520

gaccaccucu cagacuauga uaucgaccau auaauacccc aggcauuuau aaaagacgau     2580

aguauagaua accgcgugcu uacuaguucu gccaaaaaua ggggaaaaag cgacgacgua     2640

ccuucccugg acauaguucg agcacgaaaa gcagaguggg uaagacuuua caaguccggc     2700

cucauuagca aaagaaaauu cgacaaucua acgaaagcag aaagaggagg acuaaccgaa     2760

gccgauaaag caggguucau uaagcgccaa cuaguggaaa cgcgacaaau uaccaaacac     2820

guagcccaga ucuuagaugc gagauuuaau acagaaagcg augagaacga caaaguaaua     2880

cgagauguua aaguaauuac ucugaaauca aauuuaguca gucaauucag aaaagacuuc     2940

gaauuuuaua aaguucgaga aauuaacgau uaucaccacg cgcacgacgc uuaccuaaac     3000

gccguaguag gaacagcacu ucuaaaaaag uaucccaaau uagcaucaga auucgucuac     3060

ggagaauaca agaaauacga cauaagaaag uucauuacaa acuccucaga uaaggcaaca     3120

gcuaaguauu uuuucuauag uaauuuaaug aauuucuuca aacgaguaau acguuauuca     3180

aacggaaaag ugauuguacg accuguagua gaauauagca aggacacgga agauauugca     3240

ugggauaaaa aaucaaauuu uagaacaaua uguaaaguuc uauccuaccc acaagucaac     3300

auuguaaaga aaguagaaac gcaaaccgga gguuuuagca aagaaucaau auuacccaaa     3360

ggcgauucag auaaacuaau uccgagaaaa acaaaaaagg cauacuggga cacaaagaag     3420

uaugguggau uugacucacc aacuguagca uauuccguuu ucguuguagc agaugucgaa     3480

aaaggaaaag caaaaaaacu caagacagug aaagagcucg ugggaauauc cauuauggaa     3540

cgauccuucu ucgaagaaaa uccaguggaa uuucuagaaa acaaaggaua ucacaauaua     3600

cgugaggaca aacuaauuaa acugcccaaa uacucauuau uugaauucga aggcggaaaa     3660

cgaaggcucu uagcaucugc aucugaacug caaaaaggaa acgaaauggu caucccaggc     3720

caucuaguca agcuacuaua ccaugcccaa agaauaaacu cauuuaauuc aacaaaauau     3780

uuagacuaug uaagcgcaca uaaaaaagaa uucgaaaaag uacuaucgug ugucgaagau     3840

uucgccaacc uauauguaga uguagaaaaa aauuuaucua aaauaagagc cguagcugau     3900

ucaauggaua acuucucaau agaagaaaua agcaauuccu ucauaaaccu uuuaacacug     3960

acggcacuug gagcaccagc agacuuuaau uuccuaggag aaaaaauucc ucgaaagcga     4020

uacacaucaa cuaaagaaug cuuaaacgcc acucuaauac aucagucaau uaccggguua     4080

uacgaaacua ggauugauuu aagcaaaaua ggagaagaau ga                        4122


<210>  5485
<211>  4122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5485
auggaaaaca aaaacuauag uauagguuua gauauaggaa caaauagcgu uggcugggca       60

guuauaaccg augacuacaa agugccaucc aagaaaauga aaguauuagg aaauacagac      120

aaaagauuua ucaagaaaaa uuuaauagga gcacuauugu uugacgaagg uacaacugcu      180

gaagcacgca gacuaaaaag aacagcacga cgccgauaca cucggaggaa aaaucgcuua      240

agauaccugc aagaaauauu cgcugaagaa augucgaaag uugauucguc auucuuccau      300

agacuagacg auagcuuuuu aauuccagaa gacaagaaag gaucaaaaua cccaauauuu      360

gcaaccuuga ucgaagaaaa ggaauaccac aagcaguuuc cuaccauaua ccaccuacgc      420

aagcaacugg cagacaguaa agagaaaacu gaccuaagac uaauuuauuu agcccuagcc      480

cacaugauua aauaccgagg gcauuuccua uacgaagaua cauuugauau uaaaaauaac      540

gacauacaga aaauauuuaa cgaauuuaua aguauauaua auaacacauu cgaaggcaau      600

ucucuaucag gccaaaacgu acaaguagaa gcuaucuuca cagauaaaau aagcaaauca      660

gcgaaacgcg agagaguauu aaaacuauuc ccagacgaaa aaucuacagg auuauuuuca      720

gaguuccuaa aacuaauagu aggaaaucaa gccgauuuua agaaacacuu cgaccucgaa      780

gaaaaagcac cuuuacaauu uucaagagau acuuaugacg aagaucuaga aaaucuucuu      840

ggacaaauag gagaugauuu ugcagaccug uucgucgcag ccaagaaauu auacgacgcg      900

auucuuuuaa gcggaauacu aacaguuacg gacccuagca caaaagcacc ucuaucagcu      960

ucuaugauug aaagauacga aaaccaucaa aaagaucuag caacucugaa acaauuuauc     1020

aaaaccaauu uaccagaaaa auacgacgaa guauucucug accaaucuaa agacggauac     1080

gcuggauaca uugacggcaa aacaacacaa gaaaguuucu acaaauauau caaaaaccua     1140

uuauccaaau ucgaaggagc cgauuauuuc cucgagaaaa uagaaaggga agauuuccua     1200

cgaaaacaaa gaacuuucga caacggcagu auaccccauc aaauccaucu ccaagaaaug     1260

aacgcaaucu uacggcgaca aggggaacau uacccguuuc uuaaagaaaa uaaagaaaag     1320

auagagaaaa uacucacuuu ucgaaucccu uacuauguag gucccuuagc aagaggaaac     1380

cgagacuuug cuuggcugac ccgaaacuca gaucaagcca ucaggccuug gaacuucgaa     1440

gaaaucgucg acaaagcauc cucagcugaa ucuuucauaa acaaaaugac aaacuaugau     1500

uuauacuuac cagaagaaaa aguauuaccc aaacauucuu uguuauacga aacauucgcu     1560

guuuauaaug aauuaacuaa aguaaaauuu auagcagaag gauuacggga uuaccaauuc     1620

cuggacucac gucaaaagaa agauaucuuu uauacacuau uuaaagccga agacaaaaga     1680

aaagucacag aaaaagauau aauucaauac cuacauaccg uugacggcua ugauggaauc     1740

gaauuaaaag guauugaaaa acaauucaac gccagcuugu cgacuuacca cgaccuacuu     1800

aaaauuauaa aagacaaaga auuuauggac gaccccaaua augaagaaau uuuagaaaau     1860

aucguacaua cacuuacuau auuugaagau agagaaauga uaaaacaacg acuagcacaa     1920

uacgacucau uauuugauga aaaaguaauu aaagcccuaa cacgaagaca uuauaccgga     1980

uggggaaaau uaucaaguaa acucauaaac ggcaucagag acaaacaaac aggaaaaacc     2040

aucuuagacu acuuaaugga cgacggcuau aauaaccgaa acuuuaugca acuaauaaac     2100

gaugacgaac ucagcuucaa agaaauaauc aagaaagccc aaguaguugg caaaacggac     2160

gaugucaaac aaguaguuca agaacugccu ggaagccccg caaucaagaa aggcauccuc     2220

caaucgauaa aauuaguuga cgagcuagua aaaguaaugg gucaugaacc agagucaaua     2280

guaauagaaa uggcaaggga aaaccaaaca acagcacgug gcaagaaaaa uucccaacaa     2340

cgcuauaaac gaauagaaga cucgcuaaaa auccuagcau caggacucaa cgcuaaaauu     2400

cuaaaagaac auccaacaga uaauauacaa uuacaaaaug accgcuuauu uuuauauuac     2460

cuacaaaaug ggagggauau guauacugga aaaccgcuag acauaaacca acuuuccagu     2520

uacgacauag accauauugu accucaggcu uuuauaaaag acgacagucu ggacaauaga     2580

guacuuacuu cauuaaaaga caaccgagga aaaucggaua acguaccauc cuuagaagua     2640

guagagaaaa ugaaaacguu uuggcaacaa cucuuagacu caaaacuaau cucuuaucga     2700

aaauucaaua acuuaacgaa agcagaaaga gggggucuag acgaacguga uaaaguagga     2760

uucaucaaaa ggcaauuggu agaaacaaga caaauaacaa aacacguugc acaaauuuua     2820

gacgcacgau auaacaccga agucaaugaa aaagauaaga aaaacagaac uguaaaaauc     2880

auuacacuaa aaucaaacuu gguaucaaau uucagaaaag aguuccggcu auauaagaua     2940

agagaaauaa acgacuacca ccaugcacac gaugcguacc uaaacgcagu agucgcaaaa     3000

gcaauacuca agaaauaccc caaacuagaa ccagaauuug ucuacggaga cuaccaaaaa     3060

uaugaccuaa aacgcuacau cucaagaagu aaagauccaa aggaaauaga aaaagcaacu     3120

gaaaaauacu uuuucuacuc caaccuucuu aacuuuuuca aagaagaagu gcauuacgca     3180

gauggcacca uagugaaacg cgaaaauaua gaauauucaa aagauaccgg agaaauagca     3240

uggaacaaag aaaaagacuu ugcaacaauc aagaaagugc ugucccuacc acaagucaau     3300

auagucaaga aaagagaagu ccaaacuggg ggauuuucaa aagaaucuau auuaccuaaa     3360

ggaaauucgg auaaacuaau uccuagaaaa acuaaagaca uauuauggga cacgacaaag     3420

uacggagguu uugacucgcc aguaauugca uacuccauuu uacucauugc agacauugaa     3480

aaaggaaaag ccaaacgacu uaaaacugua aaaacccuag uaggcauaac aauaauggag     3540

aaagcgaccu uugagaaaag cccuauagca uucuuagaaa acaaaggcua ucauaacgug     3600

agaaaagaaa auauauuaug uuuacccaaa uacagccucu uugaacucaa aaauggacga     3660

cgcagaaugc uugccagugc aaaagaauua caaaagggaa augaaaucgu auuacccguc     3720

cacuuaacaa cgcuauuaua ucaugccaaa aacauucacc gacuagauga accagaacau     3780

uuagaauaua uacaaaagca uagaaacgag uucaaaggau uacuaaaccu agugagcgag     3840

uucucacaaa aauacguauu agccgacgcu aauuuggaga aaauuaaaaa uuuauacgcu     3900

gacaaugaac aagcagacau cgaaauacuc gcuaacaguu ucaucaacuu acuuacauuu     3960

accgcacucg gagccccagc agcauuuaaa uuuuucggaa aagacguaga ucgcaaaaga     4020

uacacaaccg uaagugaaau acuaaacgcu acucuaaucc accaaucaau aacaggacua     4080

uaugaaacac ggauagaccu cucaaaacuu ggcgaagauu ga                        4122


<210>  5486
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5486
auggagacua aauuuacaua uuaccuaggu cuugauaugg gaacugauag ccuugguugg       60

gcuguaacag auucuucaua ccauauacug cgagcuaagg ggaaagaucu auggggcguu      120

agauuguuuc caagcgcaga gacguccguu gaacgcagaa cuuauagagu uaauagaaga      180

agaagacaaa gagaagucgc ucgaauuggg gcauugaaag aacuauuugc agaugagaua      240

gaaaaaguag auccgggauu uuucgucaga cuagaugaau cuaaauucca cauggaagau      300

cgaucagaaa auaauaaaca aaaauauggu cuauuugcag auaaacuauu uucagacgca      360

ggauauuaua aaaaauaccc cacaauauuu cacuuacgaa aagagcuacu agaaagcgaa      420

gaaccacacg auguucggcu agucuacuua gcacuauuga acauguuuaa gcaccgcgga      480

cauuucuuaa auucgaguuu acaaauagaa ggagaaaaca gcaacauagc cgaaagcuau      540

caaauguucg uagacacacu aacagaugaa gauuuggauu uucccgagca agugagugga      600

gaaagacuac aggaaauauu ccguucgucu gacucacgaa aaaaaaaagu ugaaaaccuu      660

uuagcggaaa ugaaccuauc aaaaaaacaa aaaagagaaa uacaauuugu uaaccucauc      720

ugcggguuaa agggaaaguu aagugaauua uuccccgaca aagaauauga cgaggagcag      780

aaaaaaaucg cauucucauu ucaagauuca gacuacgagg acaaagagaa cacuauacaa      840

gaauugcuag acgagucaga aaugaauaug auauaugcug uaaaagcugu acaugacucc      900

ggaguacuuu cgggcauaau gaacggugaa cgguaccuuu caguugcccg aguagcaucc      960

uacgaaaagc acaaaaaaga uuugcaaaua uuaaaagaca uuuuaaaaaa auaugauaca     1020

aaagaauacu uugaaauguu ccgaaacgaa uccgcaaaaa gugaaucuua cucagcauac     1080

gucggcucag uaaaauauca cgggacauuu agacgaggau uaucaggagg aaaacaggaa     1140

gaccuauaua aacauaucaa aaaaguccua ggaaaauuuc cacaagacga cgaaggcguu     1200

acauauaucu uagaagaaau ugaaacagaa agcuucuuac ccaagcaauu aacagcgucc     1260

aauggcguca uaccaaauca aguucaugca agggagaugc uggcgauacu caaacaugcu     1320

gaacuauacu uaccauuuuu acaggagaaa gaugauacag gcuuaucagu aagcgaaaag     1380

auacuaaaau uauucauguu ucaaauacca uauuauguag gaccuuuagg ucaagaauau     1440

aaagauaaaa aaggauauaa uguaugggcc gaaaggaaag caucaggaaa aauauacccc     1500

uggaacuuug aagacaaaau agacguuaaa aaaucugcag aaaaauucau agguagaaug     1560

guacggagau gcaccuaccu auccggggaa caagcgcuac caaaacaaag uuugcuguau     1620

gaaaaauuuc aaguacuaaa ugaacuaaau aaccuacgaa uaagaggaga aaaaauucca     1680

guaucucuga aacaagaaau auaucacgac uuauucggag auggaaagaa aguaaguaug     1740

acacgacuaa cuaauuacuu guuacaaaau gggguaaucu caggugaaga gaaagauagu     1800

ugcauuucgg gaaucgaugg aggauuucac aacuccuuau caaguguagg uaaauuuaaa     1860

ggagucuuug gagauacagu gcacacagau gaacaccgaa aaaugauaga agacaucaua     1920

uuuuggggaa cuguauucgg agacggcaaa aaauuauuaa aagaaaaagu ugaagaagua     1980

uacggaaacc aacucacaga agcacaaaaa aaaagaauau uugguuuuaa auuuuccuca     2040

uggggcagac uaucuaaagc auuccuaaca auggaaggag caaguaaaga cgacggcgaa     2100

auuaaaggau uaauacaagc aaugugggau acuaacugca accuaaugga auuacuaucu     2160

gaccguuuca cauacaaaga uaaacuaaac gaaaugguaa ccacggcgga aaaacccuua     2220

ugugagugga aaauagaaga ccuugacgaa auguauuugu cagcaccagu aaaaagaaug     2280

guauggcaaa cgauuagagu uguagaugaa guacaacaaa uaauagguug uccacccaaa     2340

cgaauuuuug uagagaugac aagaagcgac ggagagaaag guaaaaggac uguguccaga     2400

caaaaaaagc ucguagauuu auacaaagcc cucggaaaag aaggaaaaac uuggagugaa     2460

gaauuaagcg acacacccga aucgcaauuu agaauaaaaa aacuuuacuu guauuaccug     2520

caaaugggaa aaugcaugua cacaggcgag gcaauagaau uagaacaacu uaugaaugac     2580

aaucuuuacg acauagauca uauauacccc agacacuuca uaaaagacga caauauauca     2640

aauaaccucg uucuaguaaa aaaagaaaaa aacgcacaua aaucggacag uuucccgauu     2700

gaaccagaga uaagaaauaa aaugaugcca uuuuggaaau cucuuaggaa gaaagguuuu     2760

cugacugaag aaaaauacaa uagacuuaca cggaugagug ccuucacaga ugaagaaaaa     2820

gcggccuuca ucggaagaca acucguagaa acaagucaag guacaaaagc cauaacgcaa     2880

auauuugaac aagcauuugc gaauagugaa aucguuuuuu ccaaagcuuc gguagucagu     2940

gcuuuuagac aaaaguucga cugcuauaag guuaggugcg uaaauaauua ucaccaugcu     3000

caugacgcau aucuaaacau aguggcaggc aacacauaua ggguuaaauu uacaagcaau     3060

ccauuaaauu uuauaaaaga agcuaaaaaa aacccaaaua guagagagaa uagguauaau     3120

auggacaaaa ucuucgaaug ggacguaacu agaaauggag aaacagcaug ggucgcacaa     3180

acaaaagaac auccaggaac cauacaauua guaaaaaagu acuugaacaa aaacagucca     3240

uuaauuacaa aaaugguuua cgaaaaacac gggggcauaa cacagaaggc aacaauaugg     3300

agugcaaaaa aagcugaaag cgggaauacu gacgcauaua uaccagucaa aaugaacgau     3360

cccagauuag cagacgucac aaaauacgga ggaguaacga cuauagccgu agcaggauau     3420

acguuauuag aauauaaagc aggaggaaag acgguuagaa gccuagaagc auugccaauc     3480

uacuuaggag guucaaaaaa gcugacagaa gaacaaaugg uggaauauuu uagcaaugua     3540

cuacaacaag aaaauaaaga aaaagugguu agugacguua uaaucagaaa aaagaugaua     3600

ccaucaggaa gucuaaucaa guauaacgga uucuacuauu aucuagcagg aaaaacagga     3660

gauaggauaa uucucauauc ugcaguacag cuaugcuuac caauggauac gauguuauau     3720

auaaaaaaga uagaaaaagc guguucaaca ggaaauuuug aagaaaaaga uaaaaauggc     3780

aagcaggucc uaaguaaaca acagaacgag uuugucuaug aacuuauaau ucaaaaguac     3840

caacaaucua uauucaaaaa aaaaguuggg gcaauaggag acacaauaau cgaaaaaagg     3900

gaaaaauuug ccgaaauauc auuagaagaa caaugcaaag uucucaugca aauauuuuug     3960

aauuuccaau ccggaauagg ugucgaauua aaaauaauug aaggagggaa gaaaagcggu     4020

guaacuuauc uuaauaaaaa aguuaugaau guacaagagc uaaaacugau cgaucaaagu     4080

guaacaggag uauucucccg ugaaauagau uuacucacaa uauga                     4125


<210>  5487
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5487
augaagaaac guuauaguua uucuauuggu cuagauauag gaacaaauuc aguaggcugg       60

gcuguaauua acgaagacua uaaaguucca gcgaagaaaa ugacaguuuu uggaaauacu      120

gaccgaaaaa ccauuaagaa aaaucuauug ggaacaguac uuuucgacag uggagaaaca      180

gcacaagcuc gacgacuaaa acggacaaau aggcgcaggu acacaagacg cagauauaga      240

uuaugccaau uacaaaacau auucgcgaca gaaaugguca aaguugacga caccuucuuu      300

caacgucugu cugaaaguuu cuucuacuac caagacaaag cguucgauaa acacccaaua      360

uucggaaacu caaaagaaga aagagcguac cacaaaacau auccaacaau auaccacuua      420

agaaaagacc uagcagacag agaccagaaa gcugaccuuc ggcuaauaua ccuagcauua      480

agucauauaa uuaaauucag aggacacuuc cugauagaag gaaaauugaa uagcgaaaac      540

acugacguuc aaaaauuauu caucgcauua guuacaguau acaaucuacu auuugaagaa      600

gaaccuaucg cuggugaaac augcgacgcu aaagcacuau uaacagcaaa aacuucaaaa      660

ucaaaaagau uagaaucacu uauaagugag uucccgggac agaagaaaaa ugguuuguuc      720

ggaaaccugc uugcacuagc ucucggacua cggccaaauu uuaaaucgaa cuuuggacug      780

ucugaagaug caaaauuaca aaucacaaaa gacacauacg aagaagaauu agauaaccug      840

cuggcugaaa uuggcgauca uuacgcagac uuauucuuag cugcgaaaaa ucugucagau      900

gcaauucuuu uaucggacau acuaacccuu ucugacgaaa acacacgagc accacuaagc      960

gccucaauga uaaaaaggua cgaagaacac caagaagauu uagcacuucu caaaaagcua     1020

gucaaagaac aaaugccaga aaaauauugg gaaauauuuu cuaaugcaaa gaaaaaugga     1080

uaugcuggcu auauagaagg caaaguuagu caagaagauu ucuauagaua uauaaaaccu     1140

auauuaucac gucuaaaagg aggagacgaa uuucuugcaa aaaucgacag ggacgacuuu     1200

cugagaaaac aaagaacauu cgauaauggc aguauaccuc aucaaauaca uuugaaagaa     1260

uuacaugcaa uacuaagaag gcaagaaaaa uacuacccau uuuuagcuga acaaaaagag     1320

aaaauagaac aacuauuaug uuuucgcauu ccauauuaug uuggaccacu ugcuaaaggc     1380

gguaacucua guuuugcaug gcuaaaaaga agaagcgacg aaccaauaac ccccuggaac     1440

uucaaagaug ugguagauga ggaagcaucc gcacaagcau uuauagaagg uaugacaaau     1500

uaugauacuu accuacccga ggaaaaagua cuaccaaaac acucacccuu auacgaaaug     1560

uuuacuguau acaacgaauu aacaaaaguc aaauauaucg ccgagaacau gacgaaacca     1620

cuuuacuuaa gugcagaaca aaaagaagcu aucaucgauc aucuauuuaa acaaacacga     1680

aaaguaacag ucaaagaccu caaagagaaa uauuucagcc aaauagaagg uuuagaaaac     1740

gucgacguaa cagguguaga aggagcauuc aacgccagcu uagguacaua uaacgaccuc     1800

cuaaaaauua uaaaagacaa agcauuccua gacgaugagg caaacgcaga gauauuagag     1860

gaaaucguuc uaauucuaac acuauuucaa gacgagaaac uaaucgaaaa acgucuugca     1920

aaauacgcua accuauucga gaaaaguguc uuaaagaaau ugcgaaaaag acacuauagg     1980

gguuggggac guuuaucaag acaauugauu gaugggauga aagauaaagc aagcgguaaa     2040

acuauucuug auuucuuaaa agcugaugac uucgcaaaua gaaauuuuau acaauugauc     2100

aacgacucua gccuagauuu ugaaaaacug auugacgacg cccagaagaa agcuauuaaa     2160

cgagagagcu uaaccgaagc cguugcuaau cuugcaggau caccagcaau uaagaaaggg     2220

aucuuacaaa guuuaaaagu aguagacgaa auagucaaag uuaugggcca uaauccagau     2280

aacaucguaa uagaaauguc cagagaaaac caaacgacug cucaaggacu caaaaaugca     2340

agacaacgac ugaaaaagau uaaagaaguu cauaagaaaa cagggaguag gaucuuagaa     2400

gacaacucug aaagaauuac gaaccuuacu uuacaggaca accgauuaua ucuauaucua     2460

uuacaagaug gaaaagacau guacacggga caagaucucg acauaaacaa ccugucgcaa     2520

uacgacauag aucauauaau uccacaaagu uucauaaagg acaauuccau ugacaaucuc     2580

guccuaacua cccaaaaggc caaucgugga aaaucagaua acguuccauc aauagaggua     2640

guaagagaca ugaaggacag gguauggcga cgccagcuag cuaacggagc aauaagcaga     2700

caaaaauuug aucauuuaac uaaagcagaa agaggugggc uagcagauuc cgacaaagca     2760

cgcuuucuca gaagacagcu aguagagaca cgacaaauca ccaaacaugu agcacagcuu     2820

cuagacucaa gauuuaauuc aaaauccaau cagaacaaga aacuggcaag aaauguaaaa     2880

auaauaacgc uaaaaaguaa aauuguaagc gauuuuagga aggauuucgg acuauauaaa     2940

cucagggagg uuaacaauua ucaccacgca cacgaugcuu auuuaaacgc uguaguaggu     3000

acagcacuac uaaagaaaua cccaaaacua gaagcugaau uuguuuacgg agauuacaaa     3060

cauuuugauc uaguuaaacu aauaaguaaa ucagacccau cacuaggaaa agcuaccgca     3120

aaaguauucu uuuauaguaa cauaaugaac uucuucaaag aagaacuuuc acucgcagau     3180

gguacucuaa ugaaaagacc agucauagaa acuaacacug aaacaggaga agugguuugg     3240

gauaaaguaa aggacuuuaa gaccaucaga aaaguauugu ccuauccaca aguaaauaua     3300

gucaagaaaa ccgaaauaca gaguggagca uucagcaaag aaagugucuu aucuaaagga     3360

aacagcgaca agcucauaga aagaaagaaa ggcugggacc caaagaaaua cggaggauuu     3420

gacucuccua acacagcaua uaguauuuuu guuguagcua aaguagcaaa aagaaaagcc     3480

caaaaauuaa aaacaguaaa agaaauagua gguauuacaa uaauggaaca agcggaauau     3540

gagaaggaua auaucgccuu cuuggagaag aaagguuauc aagacauaca ggaaaagcuc     3600

uuaauaaaac uaccaaagua cucgcuuuuc gaauuagaaa auggacgacg uaggcuucua     3660

gccucagcua augaguucca aaaaggaaac gagcuagcac uuucagguaa auauaugaaa     3720

uuucuauacc uagccagcag auaugauaaa cuuucaucaa aaauugaauc agaacaacag     3780

aagaaauuau ucguugaaca acaucugcac uauuuugacg aaauacugga caucguaguc     3840

aagcacgcaa cuugcuacau aaaagccgag aacaacuuaa agaaaauuau cucauuauac     3900

aaaaagaaag aggcuuacuc uauaaacgaa caagcucuua acaugcuaaa ccuauucaua     3960

uuuacaucac uuggggcucc cagcacauuu gucuuuuucg acgaaacaau agacagaaaa     4020

agauacacua caucaagcga uguacucaac gguauauuaa uacaacaauc aaucacagga     4080

cuauacgaaa caagaauaga cuuaucaaga uucggcggag acuga                     4125


<210>  5488
<211>  4125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5488
augaagaaac agaaauucuc cgacuacuau cuaggauucg acaucgggac aaacucaguu       60

ggauggugcg ucacggacuu ggacuacaac gugcuacgau uuaauaaaaa ggauaugugg      120

ggaagucguu uauucgauga ggcaaaaaca gccgcugaac gaagggucca acguaacagu      180

cgaagaaggu uaaaaagaag aaaauggaga cuaaaccucc uagaagaaau auucucggac      240

gaaauaauga aaauagauuc uaacuucuuc agaaggcuaa aagagucauc auuauggcuu      300

gaagacaaga auucaaaaga aaaauucaca cuuuuuaacg acgacaacua caaagauuau      360

gacuuuuaca agcaauaccc caccauauuc caccucagag augaacuaau uaagaauccc      420

gaaaaaaaag auauuagguu gauauaccua gcucuacaua guauuuuuaa aucaagagga      480

cacuuccuau uugagggaca aaaccuaaaa gaaaucaaaa auuucgaaac acucuauaau      540

aaccucauua gcuuucuaga agacaacgga auaaacaaaa gcauagauaa agauaauauc      600

gaaaaguuag aaaaaauaau cugugacagu ggaaaagggc uaaaagacaa agaaaaagag      660

uuuaagggaa uauuuaacuc ugacaaacag cuagucgcga ucuucaaacu uuccgucggu      720

agcaguguau cuuuaaacga cuuauuugac acagaugaau acaaaaaaga agaaguagaa      780

aaagaaaaaa uaaguuucag agaacaaaua uaugaagacg auaaaccaau auauuauucu      840

auauuaggag aaaaaauaga acuucuggac auagcaaaaa gcuuuuacga cuucauggua      900

cucaacaaca uacuaucaga cucaaauuac auaagugaag cuaaaguaaa acuuuaugaa      960

gaacauaaga aagaccuaaa aaaccucaag uacauaauac gcaaauacaa uaaagagaac     1020

uacgauaaac uauucaaaga caaaaaugaa aauaacuacc cagccuacau uggacucaac     1080

aaagaaaaag acaaaaaaga agucguagaa aaaucaagac ucaaaauaga cgauuugaua     1140

aaaguaauaa agggcuauuu accaaagccu gaaaggaucg aagagaaaga uaaaacaauc     1200

uuuaacgaaa uucuaaacaa gauagagcug aaaaccauac ucccaaaaca aagaauuucu     1260

gauaacggga cacuaccaua ccaaauacac gaagucgagc uagaaaaaau auuagaaaac     1320

caaucaaagu acuaugacuu cuuaaauuac gaagaaaacg guguaagcac uaaagacaaa     1380

cuacuaaaaa ccuuuaaauu uagaauacca uacuacguug gcccucucaa cucauaccau     1440

aaagacaaag gcggaaauuc auggaucguc agaaaagaag aaggaaaaau ucuaccgugg     1500

aauuuugaac aaaaagucga cauugaaaaa agugcagaag aauuuaucaa acgcaugaca     1560

aauaagugua cauacuuaaa uggcgaagac guuauaccca aagacucguu cuuauacagu     1620

gaauacauaa ucuuaaacga acuuaacaaa guacaaguaa acgaugaauu uuuaaacgaa     1680

gaaaauaaaa ggaaaauaau agacgaacua uucaaagaaa acaagaaagu aucagagaaa     1740

aaauucaagg aauaccuuuu aguaaaccag aucgcuaacc ggaccguaga acuuaaaggc     1800

auuaaagacu ccuuuaauuc aaauuaugua uccuacauua aguuuaaaga uauauuugga     1860

gagaaauuaa acuuagauau cuauaaagag auuuccgaaa aaucaauacu auggaaaugc     1920

cuauauggag augacaaaaa gauauuugaa aaaaaaauua agaaugaaua uggugacaua     1980

cuuaacaaag acgaaauaaa aaaaauuaau ucauucaaau ucaacacaug gggacgccug     2040

ucagaaaaac uacugacagg gauugaauuu aucaaccuag agacggggga guguuacucc     2100

agcguaaugg aggcacuaag aagaacuaac uacaaccuga uggaacuauu gaguucgaaa     2160

uuuacgcuac aggaaucaau cgauaacgaa aacaaagaaa ugaaugaagu gagcuaccgg     2220

gaucuaauag aagagagcua cguaaguccc ucccuaaaac gagccauacu ccaaacauua     2280

aagauauaug aagaaauaaa aaaaauaaca ggucgcguac caaaaaaagu auucauugaa     2340

auggccaggg gcggggacga auccaugaaa aacaagaaaa uaccagcgcg acaagaacag     2400

cuuaaaaaac uauaugacuc guguggcaau gacauagcua auuucucaau agacauaaaa     2460

gaaaugaaaa acucacuguc auccuacgac aauaacucau uaagacaaaa aaaauuauau     2520

cucuacuacu uacaguucgg gaagugcaug uauaccggaa gagaaauaga cuuagauaga     2580

cuucuacaaa acaacgacac auaugacaua gaccacauau auccacguuc aaaaguuaua     2640

aaagacgaua gcuucgacaa ccuaguauug guucuaaaaa acgagaaugc ugaaaaaagc     2700

aacgaauauc cugucaaaaa agaaauacaa gaaaaaauga aaucuuucug gagauuucua     2760

aaagaaaaaa acuucaucuc ugacgaaaaa uacaaacggu uaacggggaa agacgauuuc     2820

gaacugcgag gauucauggc aagacaacua guaaaugucc gucaaacaac aaaagaaguc     2880

ggaaagaucc uucaacagau agaacccgag auaaaaauug uauacagcaa agcggagaua     2940

gcaucauccu ucagagaaau guucgacuuc auaaaaguaa gagaacuuaa cgacacacac     3000

cacgcuaaag augcuuaccu caauauaguc gcuggaaacg uauacaacac caaauucacg     3060

gaaaaacccu acagauaucu gcaagaaauc aaagaaaacu acgauguaaa aaagauauau     3120

aacuacgaua uaaagaacgc augggacaag gaaaacagcc uagaaaucgu caaaaagaac     3180

auggaaaaaa auacggugaa cauaacgcga uuuauuaaag aagaaaaagg ggaauuauuu     3240

aaucuaaacc cuauaaaaaa aggagaaacc aguaacgaga uuauaucaau aaaaccaaaa     3300

cuauacgacg gaaaagacaa caaacuaaau gagaaauacg gcuacuacac gagucuaaag     3360

gcagcguacu uuauuuaugu ugagcacgaa aaaaaaaaca aaaaaguaaa aacauuugaa     3420

agaaucacca gaauagauuc cacccucaua aaaaaugaga aaaaucuaau caaauaucua     3480

guaucacaaa aaaaacuauu aaaucccaaa auaaucaaga aaauauacaa agaacaaacc     3540

cuaauaaucg acaguuaccc auauacauuc acaggagugg auagcaacaa aaaaguugaa     3600

cuaaaaaaca aaaaacaacu auaccuagaa aaaaaauacg aacaaauccu caaaaacgcc     3660

cuaaaguucg uagaagauaa ucaaggagaa acagaagaaa auuacaaauu cauauaccua     3720

aaaaaaagaa acaacaacga aaaaaaugaa acuauagaug caguaaagga acgauacaac     3780

auagaauuca acgaaaugua cgacaaauuc cucgaaaaac ucucaaguaa agauuauaaa     3840

aacuacauaa acaacaaauu auacaccaau uuucuuaacu caaaagaaaa auucaaaaaa     3900

cuuaaauuau gggaaaagag cuuaauacua cgggaauucc uaaaaauauu caacaaaaac     3960

acauacggaa aauacgaaau aaaagacuca caaacaaaag aaaaguuauu uucuuucccc     4020

gaagacacgg gcagaauacg gcuaggccaa aguagcuuag gaaacaacaa agaacuacua     4080

gaagaaucug uaacaggacu uuucgucaaa aaaauaaaac ucuga                     4125


<210>  5489
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5489
augucaaaca aaccauacag uauugguuua gacauaggaa cuaauagcgu uggaugggcu       60

guuauaacag acgacuacaa aguuccaucu aagaaaauga aaguauuagg gaacacagau      120

aaacauuuca uaaagaaaaa ucuaauugga gcauuacucu ucgacgaagg cacuacagcc      180

gaagacagaa gacuaaaacg aaccgcccgc agaagauaua caagaagaaa gaacagacug      240

cgauauuuac aagaaauuuu cuucgaagaa augucaaaau uagauagcgg guuuuuccac      300

cgcuuggacg auuucuuuuu aauuccagaa gacaaacggg gcagcaaaua ccccauauuu      360

gccacccuag cugaagaaaa agaauaucac aaacaguuuc cuacaauaua ccaccuaaga      420

aaacaacuag ccgauucuaa agaaaaagcc gaccucagac ucauauacuu aacuuuagcc      480

cacaugauca aauaccgagg ccacuuucua uacgaagaaa guuuugacau caaaaacaac      540

gauauccaga aaauauuuaa cgaauuuaua uccauauacg acaauacauu cgaagguucc      600

ucacuaagcg gacaaaaugc acaaauagaa gcgaucuuca cagacaaaau auccaaauca      660

gcuaaacggg aacgaguacu aaaauuauuc cccgaugaaa aaucaacugg auuauucuca      720

gaauuuuuaa aacuuauagu aggaaaccaa gccgacuuca agaaacacuu ugaacuagaa      780

gaaaaagcac cacuacaauu ucccaaagac accuacgaug aagaucuaga aaauguccua      840

gggcaaauag gugacgacuu cgcugacuua uuuguugcag caaagaaauu auacgacgcu      900

auauugcuua guggaauauu aacaguaacu gacccaucaa ccaaagcacc acuaucagcu      960

uccaugaucg aaagauauga aaaucaucaa aaagaccugg cagcacuaaa acaauucaua     1020

aaaacaaauc uacccgaaaa auacguagaa gucuuuagcg aucaaagcaa agacggcuau     1080

gcuggcuaua uagacggaaa aacuacucaa gaagcauuuu acaaauacau caaaaaucua     1140

uuaucuaaau ucgaaggaac agacuacuuc uuagauaaaa uagaacgaga agauuuccua     1200

cgaaaacaac guacauucga uaauggaucc aucccucauc aaauucaccu acaagaaaug     1260

aaugcaauac uaagaagaca aggugaacau uacccguuuu uaaaagaaaa uaaagaaaag     1320

auagagaaaa uacuuaccuu ccguauacca uacuacguag gcccucuagc ccgagguaau     1380

cgggacuucg ccuggcuaac aaggaacucc gaccaagcaa uccgacccug gaauuucgag     1440

aaaauuguug acaaagcauc gagcgcugaa gauuucauaa auaaaaugac aaacuaugac     1500

uuauaucuuc cagaagaaaa aguacuacca aaacacagcc uacucuauga aaccuucgca     1560

guauauaacg aacucacaaa aguuaaauuu aucgcagaag gacuaagaga cuaucaauuc     1620

cuugacagug ggcaaaagaa acaaauaguc aaccagcucu uuaaagagaa acgcaaaguu     1680

accaagaaag auauaaucca cuaccuacau acaguagacg guuacgacgg aauagaacuu     1740

aaaggcauag agaaacaauu uaaugccagu cuaucaacau accacgaccu auuaaaaauc     1800

auaaaagaca agaaauuuau ggacgauucu aaaaacgaag caauucuaga aaacauaguu     1860

cauacauuga ccauauucga ggauagagaa augauaaaac aaagauuagc ccaauacgau     1920

agucuauuug acgagaaagu aaucaaagca uuaacucgcc ggcacuauac aggauggggc     1980

aaacuuucag ccaaacuaau caacgggauc ugugauaaac aaacagguaa caccauacuc     2040

gacuaccuaa ucgaugacgg ggaaaucaac aggaauuuca ugcaauuaau caaugacgac     2100

ggccucucau uuaagaaaau aauucaaaaa ucacaagucg uuggcgagac ugacgaugua     2160

aaacaaguug uccgagaauu accagguagc ccggccauua agaaagguau acuacaauca     2220

auuaaaauag ucgacgaacu cgugaaagua auggaccaug ccccggaauc cauaguuauc     2280

gaaauggcca gagaaaauca aacaacggca cgaggcaaga aaaauaguca acagcgauau     2340

aaaagaauag aagacagucu caaaaaccuc gccucagguc uagacaguaa uauacuaaaa     2400

gaaaacccga ccgacaacau ccaacuuaaa aacgacagau uauuccuuua uuaccugcaa     2460

aauggaaaag auauguacac aggcgaagca cuagauauua aucaauuauc auccuaugau     2520

auagaucaca uuauuccaca agcauucaua aaagacgauu cauuagacaa uagaguauua     2580

accaguucaa aagacaauag gggaaaaagc gacaauguac caucccuaga agucguacag     2640

aaaagaaagg cuuuuuggca acaacuucua gauucuaaac uaauaagcga aagaaaauuu     2700

aacaacuuaa cuaaagcuga acgugguggg cucaaugaac gagauaaagu uggauucauc     2760

aaacgccagc uuguagaaac cagacaaaua accaaacaug ucgcucagau ucuggaugcu     2820

agauacaaca cugaaguaaa cgagaaagac aagaaaaaua gaacaguuaa aauuauaacu     2880

uuaaaaagca aucuaguaag caacuucaga aaagaguucc gacuauauaa aguaagagaa     2940

auaaacgacu aucaucaugc acacgaugcu uacuugaacg caguaguugc aaaagcgaua     3000

cuaaagaaau acccaaaacu agaaccagaa uuuguauaug gagaauacca aaaauaugac     3060

cucaaacgcu acaucucacg aagcagaaac ccuaaagaag ucgaaaaagc aacagaaaaa     3120

uauuucuucu acaguaaccu ucuaaacuuc uucaaagaag aagugcauua cgcagauggg     3180

accauaguua aaagagaaaa cauagaauau ucaaaagaca caggagaaau cgcauggaau     3240

acagaaaaag auuucgcaac aaucaagaaa auauuaucau acccacaagu aaauauaguu     3300

aagaaaacgg aagaacaaac agugggacaa aauggaggac uauucgauaa uaauauaguc     3360

ucuaaagaaa aaguaguaga cgcauccaaa cuuauaccca ucaaaucagg ccuaaguccc     3420

aagaaauaug gaggauacgc uagaccaaca auagcuuacu cuguauuagu uacauacaaa     3480

caaaacaaca aaaucaaaaa cacaauggua ggcaucccug uauuaacgaa acuagaauuu     3540

gaacgcgauc ccaauuuaua cuuaaggaau uugggaauua acaacguauc ccacguaaua     3600

aaauuaguca aauauacagu ccuugaauuu aagcacaaau acgguacgua cagacgguuc     3660

auaguaagua accaggaacu aaaaagagca aaucaaaucu ucguaacaac aaagcaaaug     3720

aaacuaauau cucaguauac cacacuugac aaaaaugaac augauagucu acugaaaaau     3780

aaucaacaac uuauacaauc uaucuggaac caauuucuag aguucaugcu agcgacugac     3840

cuggucaaua agaaacaaua cgaugaauua auauuaacaa acaaccaaac aauagaagag     3900

ugcagaaaua uuaugaaucg agucaucaca acccuuaguu uuacgaauau uaacgccgga     3960

cagaagaaaa uuaucuucga ugacacucua ucuuuaccaa uaaagagaug gcaauucacu     4020

gacucugaca aaauggucaa gaaaauaauu gaaagcacac uaauacacca aagcaucacu     4080

ggacuuuacg aaacaaggau ugaccuauca aaacucggag aagacuga                  4128


<210>  5490
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5490
augaaagaau accauaucgg ucucgacaua ggaacaucga guauaggcug ggccgucaca       60

gacagucagu uuaaauuaau gaggauaaaa gggaaaacag caauaggagu ccgacuauuu      120

gaagaaggga aaacagcagc agaaagaagg acauuccgca caacacgacg acgucuuaaa      180

cgcaggaagu ggagguuaca uuauuuagau gaaaucuuug caccucaucu acaagaagua      240

gacgaaaacu ucuuaagaag gcugaaacaa ucaaauauac acccagaaga uccagccaaa      300

aaccaagcau uuauaggaaa acuacuauuc ccagaccuau uaaagaaaaa cgaacgagga      360

uauccaacau uaauaaaaau gcgagaugaa cuaccaguag aacaacgagc acauuaucca      420

guaaccaaca uauacaaacu aagagaagca augauaaacg aagaucgaca auuugauuua      480

cgcgaaguau accuugcagu acaucauauc guaaaauauc gaggucauuu ucuaaacaau      540

gcgagugucg auaaauuuaa aguagguaga auagauuucg acaaaucuuu uaacguuuua      600

aacgaagcau augaggaacu ccaaaaugga gaagggagcu ucacaauaga accaucgaaa      660

guagaaaaga uuggacaacu ccuauuagac accaaaauga gaaaacucga ccgacaaaaa      720

gcgguagcca aacuacugga aguaaaagug gccgacaagg aggaaacaaa acguaacaaa      780

caaaucgcaa cugcuaugag caaauuaguc cuuggguaca aagcagauuu cgcaacagua      840

gcaaugguaa auggaaacga auggaaaauu gaucucucgu ccgaaacaag cgaagacgag      900

auagagaaau uccgagaaga auuauccgac gcacagaacg acauauuaac cgagauuacu      960

ucucuauuuu cacaaauaau gcuaaacgaa auuguaccaa acggaauguc aauaucugaa     1020

ucaaugaugg acagguauug gacccaugaa agacaacuug cagaaguuaa agaauauuua     1080

gccacacaac cagccagcgc ucgaaaagag uucgaccaag uauacaacaa auauauagga     1140

caagcaccua aagaaaaagg uuucgaccua gaaaaaggcu ugaagaaaau auuaucaaag     1200

aaagaaaacu ggaaagaaau ugacgaacua cuaaaggcgg gugauuuucu gccaaaacaa     1260

agaaucagug cuaacggugu uauaccacau caaaugcacc aacaagaauu agaccgaaua     1320

auagaaaaac aagcgaagua cuaccccugg cucgcaacag aaaacccagc gaccggagaa     1380

agagacagac aucaagcgaa auacgaacuc gaccaacucg uaucauuucg uauuccauau     1440

uauguagggc cauuaguuac accagaagua caaaaagcaa ccucaggagc uaaguuugcc     1500

ugggcaaaga ggaaggaaga cggagaaaua acaccaugga accuguggga uaaaauagau     1560

agagcggaau cagcagaagc cuuuauaaaa cgaaugacgg uaaaagauac guaucuauua     1620

aaugaagaug uucucccagc uaauucacug uuauaucaaa aauacaaugu auuaaacgaa     1680

cuaaauaaug uccguguaaa cggaagacga uugucggugg gaaucaaaca agauauauau     1740

acugaacuau ucaaaaagaa gaaaacaguu aaagcaucug auguagcauc auuaguaaug     1800

gccaaaacga ggggcguuaa uaaacccagu guggaagguc ugucggaucc aaagaaauuu     1860

aacucgaauu uagcaacaua cuuggaccug aaaucaauag uaggggauaa aguggaugac     1920

aauagauacc aaauggaccu cgaaaacauu aucgaauggc gaucaguguu ugaggacgga     1980

gaaauauucg cagauaaacu uacagaagua gaauggcuaa cagacgaaca aagaagcgcu     2040

cuaguaaaga aaagauauaa aggauggggg agauuaagua agaaacuacu cacaggaaua     2100

gucgaugaaa auggccaacg aauaauagau cuaaugugga auacagauca aaauuuuaug     2160

caaauuguaa aucaaccggu auuuaaagaa caaauagacc aauuaaauca aaaagcaaua     2220

acaaaugacg gcaugaccuu acgugaacgg guagaaagug uacuagacga ugcauauaca     2280

uccccgcaaa acaagaaagc aauauggcaa guaguuagag ucgucgaaga cauaguaaaa     2340

gcaguuggca augcuccaaa aucgauaucc auagaguucg ccaggaauga aggaaauaaa     2400

ggagaaauaa cccgaaguag aagaacucaa cuucaaaaac ucuuugaaga ccaagcgcac     2460

gagcuaguaa aagacacauc cuuaacagaa gaauuagaaa aaguaccuga cuuaucagau     2520

agauauuacu uuuacuuuac ucaaggagga aaagacaugu acacagggga cccaauaaau     2580

uuugacgaaa uaucaacgaa auaugauaua gaccacauuu uaccacaauc cuuuguaaaa     2640

gaugauaguc uagauaauag gguauuaacg ucccgaaaag aaaauaauaa gaaauccgau     2700

caaguaccug caaaacuuua ugcagcaaaa augaaaccau auuggaauca acuccuuaaa     2760

caaggguuaa uaacacaacg aaaauuugaa aaucuuacaa uggauguuga ccaaacuauu     2820

aaauaccguu cauuaggauu uguaaaaagg cagcuaguag aaacgagaca agugaucaaa     2880

cuaacggcca acauacuugg auccauguau caagaagcug gcacugacau caucgaaaca     2940

agagcaggcu uaacaaaaca acuaagagaa gaguucgacu ugccaaaagu cagagaaguu     3000

aaugauuacc accacgcagu agacgcauac uuaacaacau uugcuggaca auaccuaaac     3060

cguagauauc cgaaauugcg auccuucuuu guauauggag aauauaugaa auuuaaacau     3120

ggaagcgacc uaaaacuaag aaacuuuaac uuuuuccaug aauuaaugga aggcgauaaa     3180

ucacaaggga agguaguuga ccaacagaca ggugaauuaa uuaccacuag agaugaaguu     3240

gcagauuauu uugauugggu acaagaccua aaauugaugu uaguaacaaa agaacuuaaa     3300

agauccaacg gaaaguuauu cgaugcuacc auacaaaaug cuaacuauac aaaauuaaau     3360

gaacgaaaga aaguauuugu uccccuaaag aaagaacuag auaaaacauu auauggaucu     3420

uauagcggaa uacagaguag cuauaugaca aucguaaaga aauuugauaa gaaaggguca     3480

acuuacgcag ugcaaagaau accaguaaug uacucaaaac uauuggaacu ugaaaaagaa     3540

caauccaaac ucaacagauu aguccaaagc auagugacaa gugauccaca agcccccaau     3600

aauuuugaag uguuaaugcc auguguucug agcggccaac ugguaauaga caacggccga     3660

aaauuuacac ugacaucaua cagauaccaa uguaguacag cacagcucuu uuuaccaaag     3720

aaagaccuga aauauauaca aaacaauuuc aaagaacuua agaaaacgcc cgauaaacaa     3780

gauaaaauau uugaaacuau auaugacgau aucuuaaaac aaguagauaa auauuucagc     3840

uuauaugaca uuaacaaauu ucgauccaaa cuccaugaug guaaagacaa auuucuagca     3900

cucgaaaacu ucggagaaaa agaaagcaaa gauaaaguca uaacaaaaau ucuuagaggc     3960

cuacaugcua acgcagaaag aacaucccua aaagaaauag ggcugagcac uccacuaggu     4020

caauuucaaa guggcgaagg gggaauuacu cucagcgaca acaccaaaau aauauaccaa     4080

aguccuaccg gucuuuuuga acgccauguc uaccuaaaag accuauga                  4128


<210>  5491
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5491
augagaaaga aucaggaacc auauaauaua ggauuagaua uaggaaccag uucaauagga       60

uggucaauua ugaaugauaa cuucgaucuc augagaguua aaggcaaaaa aggcauugga      120

guccgucuau auaaugaagg gcaaucggcg gcggagagaa ggaugcaccg aacagccaga      180

cgaagauaug ggcgaagaaa auggcgacua agauuacuug aagacuuuuu ugaugaacac      240

auggccgaag uagacgacac auuuuucgcg cgacuaaaag auucaaauau aaguccgaag      300

gacgauaaaa aauacagaaa aagucuuuua uuuccgaaau caaaaggugu aacguaucaa      360

gaugauggcg aauuuuauaa aaaauauccu accauguacc auuuacggua cgccuugaug      420

acugaacauc guaaauuuga cuugcgagag auauacuuag cauuccauca uaugguaaaa      480

uauagaggaa auuuucuaua ugacacaagc gucgacucgu ucgaggcaaa gaaucucgau      540

auaaaaggaa aauucgauga aauaaaugac uuacucucau cauacacaga cuuuuaugua      600

gauaacucaa acgcugcacu ugucgaguca auccuguuag aaaaaaacac aacaagaaaa      660

gauaaaucaa aaaaaaucgc uaaauuauua cacguagaag auaaagaaaa aggcaaaaac      720

aaaaaagcaa aagacuuagc cacucaaaua ucgaaugcag uauuagguuu aaaaugcaac      780

uuccaccuaa ucuucgaguu acaacaaaaa uacucauuug auuuaugcuc agaaaaaacg      840

gaagaaaaca ucgcuaaauu aagugaagug cuagacgaaa accaaaagau gcuacucaug      900

auacuaaaag aaguccagga ucaaguaaug cuuaaugcau ucguaccuag uggaaugagu      960

cuuagcgaag cuaugaugca aaaauaugau gauuacggug aacaacuuaa aauauaccac     1020

gaacuagaac acaguguaag cgaugacagc gcagccaaac uaagaggcgc auauagagau     1080

uauaaugaca acauaauaaa acgcaucgau ggagacaaca aaaaagacac uuucuacaaa     1140

agaguuaaaa auauucuaac aaaaauaucc aaggaguaug uagaccaaga uguauuagaa     1200

ucauguggua aauuaaaaaa acuaauagau gaaaacaaac uauuuaucag acaaagaaca     1260

gcggcaaacg gcguucuacc ccaccaauua caucaaauug aaaugagaaa aauuauugau     1320

aaucaaaaag aguauuaucc uugguuagcg gaaccaaacc caaacgaaaa acgucgcgua     1380

uauaguaaau auaaaguaga agaguuaaua gcuuuuagaa uccccuauua uguaggacca     1440

uuaguggauc caaacaacgc agacaaaaac aaagaagcac gauuuuccug gaugguaagg     1500

aagaaagaug gagagauaac uccauggaau uuuuaugaca aaguugauug ggcagaaucc     1560

gcgaacaacu ucauagaaag aaugaaauca aaagauaccu acuuacuagg ggaagacgug     1620

guacccaaag aaucaaugcu guaccaaaaa uaugaaguac uaaaugaacu aaauaauuua     1680

agaauaaaug acucaggguu aucagacagu uuugaagacg uaaaacucaa acaagcaaua     1740

uacaacgauc uauucaaaaa acagaaaauu guaaagauua ccgauuuaca aaauuacuua     1800

guccagaacc acaaauauuu aguuaaacca aagauaagug ggcuagcaga cgaaaacaga     1860

uucuuaucaa gccuauccac auacagugau cuaaaaacca ucuuugggga uaaaguagau     1920

gaccgaacuu auuuuaauga cuucgaaaaa augguagaau acagcacagu auuugaagac     1980

ggacaugauu acaaccaaaa acuugaugaa uacacauggc uuacaaaaga agaaaaauac     2040

aaaaucggaa aaaaacgaua uagagguugg ggcaaauuaa guaaaaaauu auuaacggga     2100

cuaagggaua aaaacaauca uacaauaaug gacaaccuau gggaaacaaa ucgaaacuuc     2160

augcaaauac aaacugcaga ugaauuuagu aaacaaauag cagaagagaa cgagagacac     2220

uuaaaaggau caguuaguga cgcaauaaau gacauguaca cauccccugc aaacaaaaaa     2280

gcaauaagac aaguucuucg aguaguggau gauauacaaa aagcaauggg cuacgcacca     2340

ucuaguauau ccuuggaauu ugcaagagaa gauggcccau caguacgaac cguuucacgg     2400

gccaauagaa ugaagucuau uuacgaaaaa uacgcuagug aagucaguga ggaaguaaug     2460

aaagaucucg auggaguaau aaaggauaaa aaggguuuaa augaucgacu uuaccucuau     2520

uuugaacaac aaggaaaaga cauguacucg ggucacccgu uagacuucga caagguaaua     2580

uccggccaag aauacgauau agaucacauu uuaccccaag caguaaucaa agaugauuca     2640

cuagacaaca gaguucugac gacuaaagcc cuaaacaaug acguaaaauc uaaaggagua     2700

ccaugcagaa uguuuaaugg uaugcacucu uuuuggaaaa accuauauga caaaggauuc     2760

aucagcagaa gaaaauuuaa uaauuuaaca acaaauccag aaaacauaga uaaauacaaa     2820

augaaaggau uuguuaaccg acaacuggua gaaacucgcc aaauuauaaa auuaguugca     2880

aauguacuaa augacaaaua ucaaaacgau gacgucgaca ucauagaggu gcgugcggaa     2940

cugacacacg acgucagaaa gcauuucaag uucuauaaaa accgcaacgu aaaugauuac     3000

caccacgcau uugaugcaua ucuaaccuca uucauaggac acuaccucuu caaaaaauau     3060

ccaaaccugc gaccgcuuuu cgacuacaac gauuuuauga aggugaguga caacguauuu     3120

aaacaacucc gagguaauaa uuuccuaggc gaauuucuca acaaaacagg cgauauaauu     3180

agcacagacg gaaauuucgu auuaaacaaa gaagagauga uaaauaaauu gaacaaagcg     3240

uaugccuuua aaaaaauauu aguuacuaaa gaaguaggcc aacgaacugg agcaauguuc     3300

aacgaaacac guuacccugc accaaacagu aggaaagcag ccuuaaaaua uagacgcaug     3360

ccgaaaccag acucacuuau aucaguaaaa gacuauaaaa auaccgauau auauggagga     3420

uauucaggaa aaaaugaugc auauaugguc auuguagaca ugggaaaaca auacauggua     3480

guagggguac ccguuagaua cacugaaaaa cuggauaaac uuagaauaaa gcauucagaa     3540

cuauacagag augaguugcg aaaaguacuu ucaagagaua agacauuauu agacucaaaa     3600

gggaacguua aaagauuuga uauaguucua gaccgcgucc uauacggaca auuaauugaa     3660

gacggaaaug aauuauucac auuagguagc aguaaauaua aaagaaauuu cagacagcua     3720

uuuuuggaca agcaguguau agaaauacuu gacuccuccg caacaccuca accuacggac     3780

gaagaacuca uauggguaua ugaccagaua cuagauaaag uugacaagua cuucgaacua     3840

uaugauauua acagacaacg ggaaacgcuc agaaaaggac gagcugcauu uuguucacuc     3900

ccaaacauau caaugaaaaa agaugacguu acaaaaaaga aaauacuaaa cgagauacua     3960

gucgcauuac acgcaaauga aucagaaucc aacugugaaa aaaucgguaa aaaagucuuc     4020

ggacguuugc aaguaccagg ugggauaaaa cuuucaaaag aagccaaacu uauauaccaa     4080

ucaccaacag gcuuauuuga acgaguugua uacuugaaag accuuuga                  4128


<210>  5492
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5492
auggaaaaca agaaauauag cauaggucuu gacauaggaa ccagcaguau cggauuugcc       60

guaguagaug aaaacaauag aguaaugcgu gucaaaggua aaaacgcaau cggaguaaga      120

cucuucucgg agggacaacc ugcggcugau cguagaaguu uucgaacaac aagaaggaga      180

uugucucgac gaagauggag auuacgacua cuucgagaaa uauucgaacc uuacaucaac      240

cccguagaug aagcauucuu uaugagacuc aaagaaagca acuuaucacc uaaagaugca      300

aacaaacauu auucuggaga cauuuuauuu aauaaucaaa ccgaccaaga uuuuuacgaa      360

aaauacccaa cgaucuacca uuugagacac gccuuaauga cagaacacag aaaauucgau      420

guaagagaaa uuuacuuagc aauccaucau aucguaaaau ucagaggaca cuucuuaaau      480

gccgccagug cuucaaacuu cacugucgga aaacuaaacu uagaaggaaa auuugaugca      540

cugaaugaaa uauaucagag aguccuuacu gaugacccau ucgcauugag aacagauaac      600

uuagaccaca uaaaagaaau ucucauugau aacaaaagga gucaagcaga ccgccaaaga      660

acauuaacug gagacgucua ccaguccucc gaagaaaaag aaauagaaaa gaggaacaaa      720

gccauagcaa cagaaauacu uaaagcagca gucggaaaca aagcaaaacu uaauguaaua      780

acgaacguag aaguggauaa agaagcagcu aaagauuggu cacuaaccuu cgauagugag      840

accaucgaug augaauuagc caagguugac ggccaaauga cugaugacgg acgcgaaaua      900

auagagauau uacgaucacu auacaguggu auaacacuag ccgcaauugu ccccgagaau      960

cauacucuuu cucaaaguau gguggaaaaa uacaacucac acaaagcaca ucucaaacuc     1020

cuaaaagauu uaauaaauaa caugacagac acuaaaaagg cuaaaaaucu cagggcagca     1080

uacgauggcu auauagaugg uguaaaagga aaaguacuua caagagauga cuucuacaaa     1140

caaauucaag gaaauuuaga cgauucugca gaagcacaga aaauccaaga uguuauggac     1200

caagacgaau uuaugccaaa acaaagaaca aaagccaacg ggucaauacc ccaccaacua     1260

caacaacaag aauuagacca aauaguagag aaucaaaaag aauacuaccc auggcuagcu     1320

gaagaaaacc caaaugaaac aagacguggu guugcuaaau acaaguuaga ugaacuugua     1380

accuucagag ucccauauua cguaggaccg augauaacug ccgaagacca agaaaaacaa     1440

ucaggagcgu ccuuugcuug gaugaaaaga aaagaaccag guaacaucac cccauggaac     1500

uuugaucaaa aaguugacag aauggcgucu gccaaucaau ucauaaaacg aaugacaaca     1560

accgauacgu accuacuagg agaagacguc uuaccggcac aaucacuuuu auaucaaaga     1620

uuugaaguac uaaaugagcu aaacaaaauu cgcauagauc gcaaaccuau aucgauagag     1680

cuaaaacagc aaaucuuuaa cgaucuauuu aagaaauuca aaaacguaac cauaaaacau     1740

uuacaagaau aucuuguugc uacuggagga uaugauaaaa ggccuagaau ugauggcuua     1800

gccgacgaga aacgauuuaa cucaggguua ucagccuauc acgacuuaca uggaauuuua     1860

ggagcagaag uaguagaugc aaaugacaaa caagaugauc uugagaaaau uauagaaugg     1920

agcacaaucu uugaagauaa gaaaauuuac agagcuaaau uggacgaacu uacauggcua     1980

accgauuccc agaaagagaa auuggcaaca aaacguuacc aaggaugggg aaggcuaagc     2040

aagaaauuac uaucugguuu acgcaauucu gaucauagau caauacuuga uauacuuugg     2100

aucacgaacg aaaacuuuau gcaaauacaa gcagauccag auuuugcaga auuggucaaa     2160

aacgcuaacc aaggaaugcu gggcaaaaau aacucucaag acguaauaaa cgauuuauac     2220

acaucaccuc aaaauaagaa agcaauaaga caaauauuau uaguaguaca ugauauucag     2280

aaugcuaugc acggacaggc accggccaaa auucauguag aauuugcccg uggagaagaa     2340

agaaauccaa ggagaucaau ucaacgacaa caucagauag aagcagcgua cgacaaagua     2400

acaucugauc uauuagugga cgcaggagua cgucaagaac ugaaagaugc gauaaacgac     2460

aaaagagacu uuaaagaucg cuuauuccua uacuuuaugc agggaggaag agacaucuac     2520

aauggagagc ccaucaauau agacagauua ucaucauaug auauagacca uauucuccca     2580

caggcauucg ucaaagacga uucacugaac aaccgcgucc uuacagacgc ugaaggaaau     2640

agaaggaaau cggacgcagu cccgagugag cucuuuggag caaaaaugag guccacaugg     2700

gaacuaaugc gggaacaagg cuuaauaagc aaagcaaaau aucgcaacuu aacaaugaac     2760

ccagaaaaca uaaacgcgca uaccgagaac ggcuuuauaa acaggcaauu aguugaaaca     2820

cgacaaguua uaaaacuagc aguaaacauu cuagcagacg aauauaccga agacacccaa     2880

auuguaagug ucaaagcuga cuuaagccau caaaugagag aagauuucga auuacuuaaa     2940

aauagagaug uaaaugacua ucaccaugcu uucgaugcuu aucuugcagc uuucauagga     3000

aauuaccuac uugaacgaua cccuaaauug caaucauacu uuguauacgg agauuuuaag     3060

aaauuuagcc aaaaagacac caaaaugaga agauucaauu ucauuuacga ucuaaaacau     3120

ucagaucaaa uagcagauaa aaauggaaag aaaauauggc auaaaacaaa cgaucuagac     3180

uacauacgac aucuguuugc uuauaagaaa aucuuaauau cucaugaagu uagugaaaga     3240

cgaggagcgc uauauaacca aacuguguau aaagcaucgg augacaaggc uucaggucag     3300

ggcaguaaga aacucauccc gauaaaagcu aacaaaccaa cugaaauaua uggcggauau     3360

acaggaaaaa cccuagcuua uauggcaaua guaaaaguua ucaaaggaaa gaaaaucgcg     3420

uauaaagugg uggguguucc cacguccgca cuuguacaau uagcugggau agaacacggc     3480

ucccaagaag aacucaacag auuauacgac guuuuaaaac cucaguucac ucaauuaaag     3540

aaaaacaaca aaacaggaga aauaagcgaa acgguaucca aauuugaaau ugugaucccc     3600

agaguaaaau accaacaauu aguagaagac gaugauaaac uauuuaugau aggaagcgac     3660

accuauccau acaacgcaca acaacugguc cuaucagaag gggcauugaa agucauuaac     3720

aauguguaca ggaaaggaga aaauucagau aaacaagaau ccuauaaacu cgacgacgua     3780

uaugacgaaa uaauaauugc aaugaagaaa uacuuuccau uauacgacaa cagaggauuu     3840

agggaaaaag uggccgaagg aaaauccacu uucgagaauc uacccauauc aaacgaauuu     3900

aaaaacggaa aauuaacgaa aauaggaaag aaagaaguua ucaauagaau guuacaagga     3960

cuacacgcga augccaccau gacagaucua aaaauacuag gaauaaaaag uccuuucggu     4020

uuuaugcaac aaccaacugg aauagccuua ucugaaaaug cacagcucau uuaucagagu     4080

ccaacggauu uauuugaaag aagaguccaa cuaaauaaaa uuaaauga                  4128


<210>  5493
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5493
augaagaaaa uucucggcuu agaccugggc acaacaagca ucggcugggc ccucguauau       60

gaaagugaaa acacggacga aaaaucgagc auuguacgac uuggaguacg agucaacccc      120

cuuucagucg acgaaaaagg aaauuucgaa aaaggaaaag cgauaaccac caacgcugac      180

cgccaacaaa cacauggagc aagaaucaau uuacaaagau acaaauuaag acgcgaaaac      240

cugaaagauu gucugcaaaa acaaggaugg aucgguacag aagcccuaug ugaagaagga      300

aaaacgagua cauuugaaac auauaugcua cgagccaaag cagccgaaga agaaaucagu      360

cuacaugaau uaugccgagu acucuucaug augaauaaga aacgaggcua caaaucuaau      420

cggaaagcaa acaacaaaga agauggaaaa cucuucgacg ggaugacagu ggccaagaaa      480

cuguaugaag aacaucucac accagcggaa uauaguuucc aacuauuauc aagcggaaag      540

aaauuuaaac aagaauauua ucgaagugac cugaaagaag aguuugaccg caucuggaac      600

gaacaacaaa aauauuaucc agaaaucuua acggaugauu uuaaacaaca acuacaagga      660

aaaaguaaag gaaacacguc aaaaauuuuc uugggaaaau auggaauaua cucagcagau      720

cucaaaggac uagaccguaa acuacagcca cuaaaauggc guguagaggc uuuaaaacaa      780

caaguagaua aagaaguauu agcauuuguu auaagugacc ucaacgguca aauagcaucu      840

acaucaggac uccuaggagc cauaucugau agaaguaaag aacuauacuu uaacaaacaa      900

acaguuggac aauaccuaug gacuucauua aaagaaacac cccauguauc aauuaaaaac      960

aaaccuuuuu acaggcaaga uuaccuugac gaguuugaga aaauauggga aacucaagca     1020

acauaucaca aagaauuaac ucccgaauua aaacaagaaa uacgagauau aauaauauuc     1080

uaucaacgac cauuaaaauc caagaaaggc uugauaucag uaugcgagcu agaacaaaga     1140

aaagucaaag uuaccauuga uggaaaagaa aaagaaauaa guauuggccc aaaaguagca     1200

ccgaaaucau cccccauguu ucaagaauuu cguauauggc aaaauuuaaa caauauacuc     1260

uuaauagaua aagguaacaa uucaauacac ccacuauaug aagaagagaa aaacuugcua     1320

uacgaagaac uaagcguaaa ggagaaguua aguaaagcag aagcccuaaa agucuugaau     1380

aaaaacagca aacaauggga ucuaaauuac aaagaaauag aagguaaccg cacccaagca     1440

guccuuuacg auuguuacaa uagaaucauu guaaugacag gacaugaaga guacaauuuc     1500

aagaaauuaa aagcaagcga aauaagaagc uaugugaaaa cgacauuuac cgguuuaggu     1560

uuuaacacgg acauccuaga auuugauagu ucacuuacag gucaugaacu agagaaacaa     1620

cccauguauc aacuauggca cuuacucuau uccuaugaau ccgauaacag caggacaggc     1680

aaugaugccc uauuacgaaa acuagaaacg gcauucggcu uuccuaaaga auaugcagcu     1740

guacucagug acguuguuuu ugaagauuca uacggaaauc uaucaguuaa agccaugagg     1800

aaaauuuuac cucauuuaca agcaggauug aacuacucac aagccuguga agaagcagga     1860

uacaaacacu caaggagaag uuuaaccaaa aaugagcuug accaaaaaga auauaaagaa     1920

aaacuagaac uucuuccgaa aaauucacua cacaacccag ucgucgagaa aauccucaac     1980

caaaugauaa acguuauaaa cgcggugaua gacgaauaug gaaaaccaga ugaaauaaga     2040

auagaauugg caagagaauu aaagaucgga gccgcagaaa gagaaaauau gaccagauca     2100

aucaaccaag gaaaugcaga aaacaaaaga auacgcgaaa uacuagaaaa agaguucgca     2160

uuaaguuaca uaaguagaaa cgacauaaua aaauacaaau uauacgaaga auuaaaaucu     2220

aacggauuua aaaccuuaua cucagauacu uacauaucaa aagauaaacu cuucaguaaa     2280

gacuuugaca ucgaacacau cauacccaaa gcgcgccuau uugacgauag cuuaagcaau     2340

aaaacuauag aagcaagaga uguuaacauc gccaaaucaa auaaaacagc auuugacuuu     2400

auaucagaaa aauacgauca acaaggaauc gaggcuuaca aaaagaaauu agauauacuc     2460

cugcaggaug gcucuauauc uaaagccaaa uacaauaggc uauuaaugaa agaagcagac     2520

auaccaucag guuuuauaga ucgugaucuc cgcgacacac aguacauagc gaaaaaggca     2580

uacgagauac uuuccgaccu gguaagaaau guaacaccca ccaccggcgc ggucacuagc     2640

agauuaagag aagacuggca gcuaguagac guuaugaaag aacuuaacuu cgaaaaauau     2700

caaaaacuag gauuaguaga agucauagaa gaucgugacg gaagaaaaau caaacgaaua     2760

aaagauugga caaaaagaaa cgaucauagg caucacgcaa uggaugcacu aacaaucgca     2820

uucacaaagc cuaguuauau acaauauuua aacaacuuaa augcacgguc agacaaaaac     2880

agcucaauau auggaauaga aaauaaagag cuacacagag aagauaauaa acuucgauuu     2940

aacgcaccau uacccaucaa cgaguucaga gcggaagcaa gaagacaccu uaaaucaaua     3000

cucguuagcg uuaaagcuaa gaauaaaguc augacacaaa auguaaaugu aauaaaaacu     3060

aaacacggag uaauaaagaa aguacaacua acaccaagag gagaauuaca uaaugccaca     3120

uuuuauggua cuaagaaaag accuauaaua aaaaugguaa aagugggugc cgcuuuagac     3180

gaaguaacaa uaaagaaagu auccaguagu uuuaucagag aggcuuuacu ggaccgucuu     3240

caugaauaua acggcgaugc caagaaagcc uucacaggua gaaacuccau agacaaaaau     3300

cccauauaua ucgacgaaga acauaagaaa acaguaccag cuuuaguuaa aaccaucgaa     3360

ugggaaaugu accaccccac uagaaaacuc auuucaccag auuuaaaaau ugacaaagua     3420

aucgauaaag gcauaaggga aauacuuaaa acccgacugg cuaaauucaa cggcgacgca     3480

caaaaagcgu uuuccaaucu agacgaaaac ccaaucuaua aagacgaagc gaagaaaaua     3540

accuuaaaaa gaguuucacu cgaaggagua uccucaguaa uaccacuaca cacacucaaa     3600

gauagggaag gaaaacuggu aguagguaaa gaugggaaac caguacucgg ggacuaugua     3660

caaacaucca auaaccacca uauagcauuc uauauggacg aaaacggaaa cuuacaagac     3720

aacguuguau ccuucuuuga agcagcagaa cgaaaauccc aaggaguacc aguaauagau     3780

aaaaauuaua accacgacaa aggguggaac uuucuauuua caaugaaaca aaaugaauau     3840

uuuguuuucc caaacgaagu gacgggcuuu aaucccagcg aaguagaccu gauggaugaa     3900

gcaaacuacu caauaaucag cccaaacuua uauagaguac agaaaauguc aaaagcauca     3960

ggaagagacu auugguuuag gcaucaucug gaagcuaugc uuaacgacga cccaaaacua     4020

aggaacucaa uguuuaaaag aauaacaagc cuaaaagcuc uagaaggaau aguaaaagua     4080

cgaauaaacu guauagguaa aauaguagcu guaggggaau acgacuga                  4128


<210>  5494
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5494
augucaaaca aaucauacag cauaggacuc gauauaggaa caaauucagu cggaugggca       60

guaauaacag augauuauaa agugccguca aagaaaauga aagucuuagg aaauaccgac      120

aaacauuuua ucaagaaaaa ucuaauagga gcauuacuau uugacgaagg uaccacagca      180

gaagacagac gccucaaacg cacggcacga agacguuaca ccagaaggaa aaauagacua      240

agauaccuac aagaaauauu uucacaagaa auaucuaaag uagacuccuc cuuuuuccau      300

agacuagacg auuucuucuu aguaccggaa gacaaacgcg gauccaaaua cccuauuuuc      360

gcaacauuag ucgaagaaaa agaauaucac aagaaauuuc ccacuauaua ucacuuacgc      420

aaacaccuug cagacucuaa agagaaaacu gaucuacgau uaauauaucu agcccucgca      480

cauaugauaa aauauagagg ccacuuucuc uacgaagaga gcuucgacau caaaaauaau      540

gauauccaga aaauauucuc agaauuuauc agcauauacg acaacacguu ugaagguaaa      600

aguuuauccg gacaaaacgc ucaagucgaa gcaauauuca cugauaaaau uucaaaaucg      660

acaaaaagag aacgaguuuu aaaauuauuc ccagaugaga aaaguacagg acuauucagc      720

gaauuucuga aacuaauugu cgguaaucaa gcugacuuca agaaacauuu ugacuuagaa      780

gaaaaagcuc cacuacaauu uucgaaagac acauaugacg aagaccugga aaacuuacuc      840

ggucaaauag gggacgauuu ugcagacuua uucuuaguug ccaagaaacu auaugacgca      900

auacuacuau cuggcauauu aacggugaaa gaucuaagca caaaagcucc auuaagugcu      960

ucaaugaucg aacguuauga aaaucaccaa aaagaccuag cggccuuaaa acaauuuauc     1020

caaaacaacc uccaagaaaa auacgaugaa guauucagcg accagucgaa agauggauau     1080

gcaggauaca uagauggaaa aacaacgcaa gaggcuuucu auaaauauau caaaaaccuu     1140

uugucuaaau uugaaggagc agacuacuuc cuagauaaaa uagaacguga agacuuuuug     1200

aagaaacaaa gaacauuuga caacggauca aucccgcacc aaauacaccu acaggaaaug     1260

aacgcaauaa uaagaagaca aggggaacau uacccauucu uacaggaaaa caaagagaaa     1320

aucgagaaaa uccuaaccuu uaggauaccc uacuacgucg gcccccuagc cagaggaaac     1380

ggagauuucg cauggcuaac ucgcaacucg gaccaagcca uaagaccaug gaacuucgaa     1440

gaaauaguag aucaagcaag cagugcagaa gacuucauca auaaaaugac aaauuacgac     1500

uuauaucucc ccgaagaaaa aguacugccg aaacauagcc uacucuauga aacuuuugcu     1560

guauacaacg aacuaacaaa agucaaauuu auagcagaag gacuaacaag auaucaguuu     1620

uuagacaaga aacaaaagaa agauauauuu gacacauucu ucaaagcuga aaacaaacga     1680

aaaguaacug aaaaagacau aauccacuau cuacacaacg uugacggcua ugacggaaua     1740

gaacucaaag gcaucgaaaa acaguuuaac gcaucauuau caacuuacca cgaucugcuu     1800

aaaauaauua aagauaaagc cuuuauggau gauucuaaaa augaagaaau acuggaaaac     1860

auaauccaca cgcuaacaau cuucgaagau agagaaauga uuaaacaacg guuagcucaa     1920

uacgauucau uauucgacga aaaagucauu aaagcucuga cuagaagaca cuauaccgga     1980

uggggaaaac ucagcgcaaa auuaauaaac ggcauuaggg acaagaaauc cggcaaaaca     2040

auccuagacu auuuaauuga ugauggggaa auaaacagaa acuucaugca acucauacac     2100

gacgacggcu uaucuuucaa agaaauaaua caaaaagcac aagucuucgg uaaaacuaac     2160

gaugugaaac aaguugucca agaacuucca ggaucuccag ccaucaagaa agggauacua     2220

caaucaauua aaaucguaga ugaauuagug aaaguuaugg gccaugcccc agaaagcauc     2280

guuauugaaa uggcuagaga aaaucaaacu acggcccgag gcaagaaaaa uucacaacaa     2340

cgguauaaaa gaauagaaga uagccuuaaa aauuuagccu cuggucuaga cucuaacauu     2400

cuuaaagaaa accccacaga caauauucaa cuucaaaacg acagauuauu cuuauauuau     2460

cuucaaaacg gcagagacau guacacagga aaaccgcuag aaaucaauca auuaucaaac     2520

uacgauaucg accacauaau cccccaagca uucauaaaag augauucacu cgacaaccgu     2580

guuuuaaccu caagcaaaga uaauagagga aaaucugaca acguaccauc acuagaaguc     2640

gucgagaaaa ugaaagcauu cuggcaacaa cuccuagacu caaaauuaau uucagaaaga     2700

aaauucaaca accucacaaa agccgaacga gaaagaggcg gccuaaauga acuugauaaa     2760

guaggcuuca ucaaaagaca auuaguagaa acacgccaga uaacaaaaca cguagcgcaa     2820

uucuuagacg caagauucaa uaaagaaguu acagaaaaag acaagaaaaa uagaaacgua     2880

aaaauaauaa cacugaaauc aaauuuagug ucaaauuuua gaaaagaauu uggacucuau     2940

aaaguuaggg aaauaaauga cuaccaucau gcacacgaug ccuaccuaaa cgcaguacua     3000

gcgaaagcaa uacuaaagaa auaucccaaa cuggaaccag aguucgucua uggcgauuau     3060

cagaaauaug aucuacaaag auauauuuca aaaagucgag aaccaaaaga aguggaaaaa     3120

gcaacccaga aauacuuuuu cuauucaaau uuacuuaauu ucuuuaaaga agaagugcac     3180

uacgcagaug gaaccauagu aaaaagagaa aacaucgaau acucaaaaga uacaggagaa     3240

auagccugga acaaagaaaa agacuucgcu acaguaaaga aagucuuaag ccuaccacaa     3300

gucaacauag ugaagaaaac cgaaguucaa acggguggau ucagcaaaga aucaauccua     3360

ccgaaaggaa auucagauaa acuaauaccu cgcaaaacaa aagaaauccu cugggauaca     3420

acaaaauaug gaggcuucga uucaccagua auagcauacu cuauccuauu aauugccgau     3480

auagaaaaag gcaaagcuaa gaaauuaaaa acugucaaaa cuuuaguugg cauuacaaua     3540

auggaaaaag caaccuuuga gaaaaaccca auaacuuucc uugaaaauaa aggauaucau     3600

aauguaagaa aagaaaauau acugugccua ccuaaauaca guuuauucga acucgaaucu     3660

ggccgccgac gaaugcuagc uucugcgaaa gaacuucaaa aaggaaacga aauaguguug     3720

cccguuuacc uaacuacccu auuguaccau aguaaaaaug uucacaaauu agacgaacca     3780

gaacacuuag aauauauaca aaaacacaga uacgaauuua aagaucuccu gaacuuagua     3840

agcgaauuuu cucagaaaua cguucuggca gacgcaaacc uagagaaaau caaaaaccug     3900

uaugcagaca augaacaagc cgacauagaa auauuagcaa acaguuucau aaaucuacug     3960

acuuuuacag ccuugggcgc cccagcagcc uuuaaguucu uaggaaaaga cguagauaga     4020

aaacgcuaca ccacagucag cgaaauucuc aacgccacac ucauacacca aucaauaacu     4080

ggauuauaug aaacgagaau agaucuuucu aaacuuggag aagauuga                  4128


<210>  5495
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5495
auggaaaaga aauacuccau aggauuagau auaggaacaa auagcguagg augggcaguu       60

auuacagaug auuacaaagu uccgagcaag aaauuuaaag uucuuggaaa cacaaaucgc      120

aaaucaauaa agaaaaaucu uaugggcgca uuauuauuug auuccggaga aacagcagaa      180

gcaacacgac uaaaacgaac ggcuagaaga agauauacaa gacgcaaaaa ccgaauuaga      240

uauuugcaag aaauuuucgc aaacgaaaug gcaaaacuag acgacucuuu cuuucaaagg      300

uuagaagagu cauuccuagu agaagaagac aagaaaaacg aacgccaccc aauauuuggu      360

aacuuagcag acgaaguagc auaccaccgu aacuauccca caauauauca uuuaaggaag      420

aaauuagcag auagccccga aaaagcugac cuaagauuaa uauacuuagc cuuggcacac      480

aucauaaaau uuagagggca cuuccuuauu gaagguaaac uaaacgcaga aaauucagac      540

guugccaaau uauucuauca acuaauccaa acauauaacc aauuguucga agaaucaccu      600

uuagaugaga uagaaguuga ugcgaaagga auacuuagcg cuagauuauc aaaaagcaaa      660

cgauuagaaa aacuuauagc uguauuucca aaugaaaaga aaaauggucu auucgggaac      720

auaauagccc uagcuuuagg uuugacucca aacuuuaaau cgaacuuuga uuuaacagaa      780

gaugccaaac uucaauuauc caaagauaca uaugacgaug accuagacga acuccuaggu      840

caaauaggug aucaauacgc cgauuuauuu agugcggcca aaaauuuauc cgaugcuaua      900

cuauuaagug acauacuacg uagcaacucu gaaguaacaa aagccccguu auccgccagu      960

auggugaaaa gguacgauga acaucaucaa gaucuagcac uucuaaaaac cuuaguuaga     1020

caacaauucc cagaaaaaua ugcagaaauu uucaaagacg auaccaaaaa cggguacgca     1080

ggguacguug gaauuggaau aaaacauaga aaacgaacaa caaaacuugc cacccaagag     1140

gaauuuuaua aauuuauuaa accaauacuu gagaaaaugg acggcgcaga agaacuauua     1200

gccaaauuaa aucgagauga ccuguuaagg aaacaaagaa cauucgacaa ugggagcauu     1260

ccacaccaaa uccaccuaaa agaacuccac gcaauacuac gcagacaaga ggaguucuac     1320

ccauucuuaa aagaaaacag agagaaaaua gagaaaauau uaacauuccg aauccccuac     1380

uacgucgggc cauuagccag aggcaauagu cgauucgcau ggcucacaag aaaaucagag     1440

gaagcaauaa caccuuggaa cuuugaagag gucguagaca aaggagcaag cgcucaaucg     1500

uuuauagaac guaugacuaa uuucgacgaa cagcuuccua auaagaaagu auuaccgaaa     1560

cauucacuau uauaugaaua cuuuacagua uacaaugagc uuacuaaagu caaauacgua     1620

acugaaagga ugagaaaacc agaauuucuu ucaggcgaac agaagaaagc cauuguagac     1680

cuauuauuca aaacaaaucg aaaagugacu guaaaacaac uuaaagaaga cuauuuuaag     1740

aaaauugaau gcuuugacuc uguagaaaua auaggcgugg aggauagguu caaugccagc     1800

uuaggaacau aucaugauuu auuaaaaaua aucaaagaca aagauuuucu cgacaaugaa     1860

gaaaaugaag acauccugga agauauagua uuaaccuuaa cucuguuuga agaccgggag     1920

augauagaag aaagauuaaa aacuuacgcc cauuuauucg acgacaaagu gaugaaacaa     1980

cuaaaacgua gacauuacac aggauggggu cgucuaucgc gaaaaaugau caacggaaua     2040

agagauaaac aaucuggaaa aacaaucuua gauuuccuua aaucagaugg auucucaaau     2100

cguaacuuua ugcaacuuau acacgaugac agccuaacau ucaaagaaga aauugaaaaa     2160

gcacaaguaa guggccaagg cgauucuuua cacgaacaaa ucgcggaucu cgcaggaagu     2220

ccggcuauca agaaagggau auuacaaaca guaaaaauag ucgacgaacu gguaaaaguu     2280

augggacaca aaccagagaa caucgucauu gaaauggcaa gggaaaacca aacgaccaca     2340

aaaggcuuac aacaaucacg agagcgaaag aaacguauag aagaaggaau aaaagaauua     2400

gaaucccaaa uuuuaaaaga aaacccagua gaaaauacac aacugcaaaa ugaaaaacua     2460

uaucuauauu aucuacaaaa cggaagagau auguauguag accaagaacu agacauaaau     2520

agauuauccg auuacgacgu ggaucauauc gucccacaau cguucauaaa agaugacagc     2580

auagacaaua aaguauugac aagaaguguc gaaaaucgag gcaaaucaga uaauguccca     2640

agugaagaag uagucaagaa aaugaaaaac uauuggcgac aacucuuaaa ugccaaauug     2700

aucacacaaa gaaaguuuga uaaccucacg aaagcagaaa gaggagggcu aucagaagca     2760

gacaaagcug gauucaucaa aagacaauua guggaaacac gacaaauuac aaaacacgug     2820

gcuagaaucc uugacucccg caugaacacu aaacgcgaca aaaacgauaa accgauuaga     2880

gaagucaaag uaauaacacu uaaaucaaaa cucguaucag acuuuagaaa agauuuccaa     2940

cuauauaaag ugcgagauau aaauaauuau caucacgccc acgacgcaua ucuaaaugcu     3000

guaguuggaa cugcccuuau aaagaaauau ccaaaauuag aaucagaauu uguguaugga     3060

gacuauaaag uauaugacgu acgaaaaaug auagcgaaaa gugaacagga gauagggaaa     3120

gcaaccgcca aacgcuuuuu cuauucaaau auaaugaacu ucuuuaaaac agaaguuaag     3180

uuagcgaacg gagagauuag aaaacgacca cuuauagaaa caaacgguga aacaggagaa     3240

gugguaugga auaaagaaaa agacuucgca acaguacgaa aaguacuagc caugccacaa     3300

guaaacauag ucaagaaaac ugagguacaa acaggcggau uuucaaagga auccaucuua     3360

agcaaacgag agagugcgaa acugaucccu agaaagaaag ggugggauac acguaaauau     3420

gguggguuug guaguccaac cguugccuac aguauauuag uaguagcuaa aguggaaaaa     3480

ggaaaagcca agaaauuaaa auccguuaaa guuuugguag guauaacuau cauggaaaaa     3540

ggaucauacg agaaagaccc uauaggcuuu uuagaagcaa aaggauauaa agacaucaag     3600

aaagaacuca ucuuuaaauu accaaaauau ucccuguucg aacuagagaa cggacgaaga     3660

agaaugcuag cuagcgccac agaacugcaa aaagcaaaug aauuaguuuu accacaacau     3720

uuaguaagac uacuauauua uacucaaaau auuagcgcaa caacaggauc gaacaacuua     3780

ggauauaucg aacagcauag agaagaauuu aaagaaauuu uugagaaaau aauugacuuc     3840

agugaaaaau auauccugaa aaacaaagug aauaguaauc uaaaaucguc uuuugacgaa     3900

caauucgcag ugucugauag cauacuacuu agcaauaguu uugucaguuu acuuaaauau     3960

acuagcuucg gagcgagcgg gggauuuaca uuccuggacu uagacgucaa acaaggaaga     4020

uuaagauacc agacuguuac agaaguacuu gaugcaacuu uaaucuauca aucuauaacc     4080

ggcuuauacg aaacuaggac agaccugucc caacuaggcg gagauuga                  4128


<210>  5496
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5496
augaagaaaa uucuaggacu agacuuagga acgacaucaa uaggaugggc ucuugucaac       60

gaaagugaaa acaaagauga acaauccuca auaguacgau uaggaguaag agugaucccc      120

cuaacaguug acgaaaaagg uaacuucgaa aaagguaaag caauaacaac aaaugcugac      180

aggcaacaag uacaugcagc aagaaucaau cuccaaagau acaaauuacg ucgugagaac      240

uuacgagacu gucuccaaaa acaaggaugg auagguagcg agccaaugug ugaagacgga      300

aaaucaucaa cauuugaaac cuauaaauug agagcaaaag cugccgucga agaggucagu      360

uuacaugagu ucgcccgagu ccuauucaug uugaacaaga aaagaggcua uaaaagcaac      420

agaaaagcca acgauaaaga agaaggaaaa uuauuugaug gaaugacaau ugcuaagaaa      480

cucuacgaag aacaucuaac ucccgcagaa uacucacuac aacuucuuaa cacugguagg      540

aaauuuacgc cggaauauua ccgcucggac cuaauagaag aauuugagaa aaucuggaau      600

gaacaacaaa aauauuaucc cgaaauacua acagaugaau uuaaacaaca auuagaagga      660

aaagcuaaau cuaacacauc aaaacucuuc cuaggaaaau augguauaua cucagcagau      720

acuaaaggac uagauagaag guuacaacca cuuaaaugga gagucgaagc caugaaacaa      780

caaguugaca aagacguccu agcauucguc aucagcgauc uuaaaggaca gauagcaaau      840

acaucuggac uauuaggagc aaucucugac cgaagcaaag aacucuacuu caguaaacaa      900

acgauaggac aauauuuaug ggcgucacua caggaaaauc cacacauaag cauaaagaau      960

caaccauucu aucgacaaga cuaccuugac gaauuugaga aaauauggga aacacaagcu     1020

acauaucaca aagaauuaac gucagaacug aaacaagaaa uaagggauau aauuauauuu     1080

uaucagaggc cauugaaauc aaagaaaaau uugauugcag uaugcgaacu agagcaaaga     1140

aaaguuaaag uaaccgugga cggaaaagaa aaagaaauaa ccaucggacc caaaauugcc     1200

cccaaaucua gcccuauguu ccaagaguuc cgaauuuggc aaaaucucaa caacaucuua     1260

cuguuagauc acgaaaccaa cucaaaaaga ccucuauaug aagaagaacg gaauuuacuc     1320

uaugaagaac uaaguguaaa agaaaaacuc ucuaaaacag aagcucuaaa aguauuggga     1380

aagaaaucca aacaguggga cuuaaacuac aaagaauuag aaggcaaucg cacccaggca     1440

guucuguaug auuguuacaa ucguauaaua guaaugagug gacaugaaga auguaauuuu     1500

aagaaauuaa aagcguccga uauacgucac uacgugaaaa ugauauuuaa aaauuuagcc     1560

uucaauacgg acaucuuaug cuucgacagc ucuuuaaaag gucaugaacu agaaaggcaa     1620

cccauguguc agcuauggca cuuauuauac uccuaugaag gagauaauuc ucgaacagga     1680

aaugaugcac uguuaagaaa auuagaaacu ucauuuggau ucccgaaaga guaugcugca     1740

guauuggcug acguaauuuu ugaagauagu uacggaaauc ucagugucaa agcaaugcga     1800

aacauacuac cccaccuaca agcaggcaaa gauuauucac aagcauguga agaagcagga     1860

uauaaacaca gcagacgauc auuaacaaaa gaagaauuaa aucaaaaagu uuacaaggaa     1920

aaacuagaac uacugccuaa aaauucauug cauaacccag uaguugagaa aauacuuaac     1980

caaaugguca acgugauuaa ugcuguaaua gaagaauaug gcaaaccgga ugaaauucgg     2040

auugaauuag caagagacuu aaaaauagga gcagccgaaa gggaaaauau gacaaggagu     2100

auaaaccagg gaaacgccga aaacaaacga auaagggaaa uauuagaaaa ggaauuugca     2160

cuuucauauu uaucgcguaa ugauauuaua aaauacaaac uauacgaaga acuaaagagu     2220

aauggauuua agacacucua cagugauaca uauaucucaa aagacaaacu cuucucaaaa     2280

gacuucgaca uagaacacau aauaccaaaa gcuagacuau ucgacgauag cuuauccaac     2340

aaaacaauag aagcaaggga uguuaauaua gcaaaaagca acaagacagc uuuugauuuc     2400

auacuggaac gcuaugggga acagggcgua aaaacuuaua aaacgaaguu acuuagacua     2460

uuggaagacg gggcaaucag caauaaaaag uauaauaauu uauugaaaac agaagcagau     2520

auaccaucug gcuucaucga ccgagauuua agaaacaccc aauauaucgc aaagaaagca     2580

uacgaaauac uaucagaauu aguaaaaaua guaacuccua ccacuggauc uguuacaaau     2640

agacuaaggg aggauuggca gcuaguugau guaaugaaag aacuaaacuu cgaaaaauac     2700

caaaaacuag gccuugcgga ugucauagaa gacagagaug gacgaaaagu uaaaagaaua     2760

aaagacugga caaaacgaaa cgaccauaga caccacgcga uggaugcccu aacaauugca     2820

uucacaaaac caucauauau acaauacuua aacaaucuca augcaagaag cgacaaauca     2880

ucguccauau acggaaucga gguaaaagaa uuacaccgag aagaaggaaa auucagauuu     2940

aacgcaccau uaccaaucaa ugaauuucga gccgaagcaa aacgacacuu aaaaucugua     3000

uuagucucaa uaaaagcuaa gaagaaaguu augacucaaa acguaaacaa aauaaaaaca     3060

aaacacggua uaauuaagaa aguacaacua accccacgag gggaacucca caacgcuacu     3120

uacuacggaa cgaagaaacg accccuaguc cgaaugguca aaguuggagc ugcacuagau     3180

gaagcuacaa uaaaaagagu cuguucucca gcaauacggg cagcacuacu uaaaagguua     3240

aaugcuuaca auggagacgc gaaagaagca uucacugguc gaaacagcau cgacaaaaau     3300

ccaauuuauc uagacauaga acagacgaaa uuaguaccag ugcuaguaaa aacaauagaa     3360

uggggaaugu accacccaau acgcaaaagg auuacuccag aucuaaaaau agacaaagua     3420

aucgacaaag gaauacguga gaaacuaaaa gccagacugg cagaauuuaa cggagaugcu     3480

caaaaggcuu uuuccaaccu ugacgaaaac ccaauauaua uggacgaagc uaaaagaaua     3540

acuuugaaaa gagucaguau agaaggagua uuaucggcua ucccacucca caccuugaaa     3600

gacaaagcag ggaaaccagu uauagguagc gauggcaaac ccguauuugg agauuacgug     3660

caaacaagca acaaccacca cauagcuuuc uauauagaug acaaaggaaa ccugcaagac     3720

aaccccguca gcuucuucga agccgcagaa cgaaaaucac aaggcguacc cgugaucgau     3780

agaagauaca acugcgauaa gggcuggcau uucgucuuua caaugaaaca aaacgaauac     3840

uucguauuuc ccaacgaagc aaccggcuuc auuccaagug aaguugaucu acuagaugaa     3900

gcuaacuauu gcaucauauc accgaaucuc uauagaguuc aaaaacugag caaggcuucc     3960

ggucgugacu acugguuuag acaccaucuu gaaacaaugu uaaacgauga ucuacgacuc     4020

aaaaacagua cauucaaacg aauacaaagu cuaaagggac uagaagacau uguuaaaguc     4080

cgaauuaaua gcauugguaa aaucguagcu guggggaaau acgauuga                  4128


<210>  5497
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5497
auggaaacua aauucacaua uuaucuugga uuagacaugg guacagacag ucuaggaugg       60

gcaguaacag acucaucaua ucauauacua agagcaaaag gaaaagauuu augggggguc      120

agauuauuuc cggcagcaga aacaucagug gauaggagaa cguauagagu gaaucgucga      180

cgaaggcaaa gagaaguugc uaggauagga gcauuaaaag aacuauuugc agacgaaaua      240

gaaaaaguag auccaggguu uuuuguccgu uuagacgaaa guaaguuuca cauggaagau      300

agaucagaaa auaacaaaca aaaauaugga cuauuugccg acaagcucuu cagcgaugcc      360

gaauauuaua aaaaauaccc cacaauauuu cacuuaagaa aagaacuauu agaaucagaa      420

gaaccacaug auguccgucu cguauaccua gcacuauuaa auauguucaa gcauagagga      480

cauuuuuuaa auagcagucu acaaauagaa ggagaaaaua guaacauagc agaaucauau      540

caaauguuug uggauacuuu aaaggaugaa gaauuagacu ucccugaaca aguaucagga      600

gaaagacuac aagaaauuuu cagaaguucu gacuccagaa aaaaaaaagu agaaaaccuc      660

uuggcagaaa ugaauuuauc aaaaaaacag aaaagagaaa uucaauucgu aaauuuaaua      720

uguggauuaa aaggaaaauu auccgaauua uuucccgaua aagaauauga ugaggaacaa      780

aaaaaaauag cuuucagcuu ucaggacuca gacuaugaag auaaagaaaa cacaauccaa      840

gaacuauuag augaaaguga aaugaauaug auuuacgcgg uuaaagcagu ccaugacuca      900

gggguacugu caggaaucau gaauggcgaa cgauaccuga guguagcucg aguugcuagc      960

uacgaaaaac auaaaaaaga uuuacaaaua uugaaagaua uacuaaaaaa auacgauaca     1020

aaggaauauu uugaaauguu caggaaugaa ucagaaaaaa gugaaaacua uucagcauac     1080

gugggcucug uaaaguacca cgguacguuu agaagagguc ucucaggagg aaaacaggaa     1140

gaccuauaua aacauaucaa gaagguacua ggaaaauuuc cacaggauga ugaaggagua     1200

accuaugucc uagaggagau agaaacagaa agcuuccuac cuaaacaacu aacugcaagc     1260

aacgggguaa uacccaacca aguacaugcg agagaaaugc uagcaauacu aaaacacgca     1320

gaguuguauc uaccuuucuu acaagaaaaa gacgauacag gucuaagcgu aucagaaaag     1380

auauuaaaau uauuuauguu ucagauaccu uacuacguag gaccccucgg acaagaauau     1440

aaagacaaaa aaggcuacaa uguaugggcg gaaagaaaag aaccagggaa aauuuaccca     1500

uggaauuucg aagaaaagau ugauguuaaa aaauccgcag aaaaauucau uggcaggaug     1560

guacgaagau guacguacuu guccggcgaa caagcauuac caaaacaaag cuuacuauac     1620

gaaaaauucc aaguacucaa cgaacugaac aaucugagaa uacgagguga aaaaauaccc     1680

gucucuuuaa aacaagagau uuaccacgau uuguucggag acggaaagaa agucagcaug     1740

acaagauuaa ccaacuaucu guuacaaaac gggguaauau cauccgaaga aaaagacagu     1800

uguauaucag gaaucgaugg uggauuucac aacucacugu caagcguugg aaaauucaaa     1860

ggggucuuug gcgauaccgu acauaccgac gaacaucgua aaaugauuga agacauuaua     1920

uuuuggggca caguauucgg cgacggaaag aaacuuuuaa aagaaaaagu ggaggaagua     1980

uacgguaauc aacuaacaga agcacaaaaa aaaagaauau ucggauucaa auucuccgga     2040

uggggacgcu uaaguaaagc guucuuaaca auggagggug ccucaaaaga uaauggugaa     2100

auaaaggggu uaauccaagc uaugugggau acaaauugua acuugaugga auuacuuagc     2160

gaaagauuua cguauaaaga caaacuaaac gaaaugguaa caacagcaga aaaaccgcua     2220

ugcgaaugga aaauugaaga ccuagaugag auguacuuaa gcgcaccugu aaaaagaaug     2280

guauggcaaa cuauuagagu cguugacgaa guccaacaga uaaugggaug cccaccaaaa     2340

agaauauucg uagaaaugac uagaucugac ggggagaaag ggaaaagaac agugagcagg     2400

cagaaaaaac uaguagaccu auauaaagcc cuaggaaagg aaggcaaaau cugguccgaa     2460

gaacuaaaua acacaccaga agggcaauuc agaauaaaaa aacucuaucu auacuaccua     2520

caaaugggaa aauguaugua cacaggugaa aguauugagu uagaaccacu aaugaaugac     2580

aaccaucuau augacaucga ucacaucuau ccccgacauu ucaucaaaga ugacaauaua     2640

ucaaauaacc uagucuuagu aaaaaaagaa agaaacgccc auaaaucaga uucauuucca     2700

auagaaccag aaauaagaaa uaaaaugaug ccuuucugga agucgcuccg aaagaaagga     2760

uuuuuaacag aagagaaaua caauaggcua acucgaaugu cagcauuuac ugacgaagag     2820

aaagcggcuu uuaucucuag acaauuagua gaaaccagcc aagguacaaa ggcuauuaca     2880

caaauauuua aacaagcguu ugccgacucu gaaaucguau ucaguaaagc cagcguagua     2940

ucagcguuua gacaaaaauu ugacugcuau aaaguuagau guguaaauga uuaccaccac     3000

gcccaugaug cguaccucaa cauaguugca gguaauacgu aucgagugaa guucacuagu     3060

aauccuuuga auuuuauuaa agaagcgaaa aagaacccca auagcaggga aaaucgcuau     3120

aacauggaua aaacauuuga augggauguu acaagaaaug gagaaacagc uuggguagca     3180

caaaguaaug agaauccagg gaccauacaa cuuguuaaaa aauauuugaa uaaaaauagc     3240

ccgcuaauca caaaaauggu auaugaaaaa cacggaggaa uaacacaaaa agccacaauc     3300

uggagugcaa aaaaagccga gaguggcaac acagaugcau auauaccagu gaaaaugaau     3360

gacccaagau uagcagaugu aacaaaauau ggaggaguua ccacaauugc uguugccgga     3420

uacacccuau uagaauauaa agccggcggg aaaaccguga gaucuuuaga ggcuuuaccu     3480

auauauuuag gaggaucaaa aaaccuuaca gaagaacaaa ugguagaaua cuuuaguaau     3540

auacuucaac aagagaacaa agaaaaagua guaagcgacg uaauaauaag aaaaaaaaug     3600

auacccucag gaagccuuau aaaauacaau ggauucuauu auuauuuggc agguaaaacu     3660

gguaauagga uuguacuaau uagugcagua cagcuauguu uaccaaugga caccauguua     3720

uauaucaaaa aaauugaaaa agcauguucg accggaaauu ucgaagaaaa agauaaaaac     3780

gggaaacaag uauuaaguaa acaacaaaac gaauucguau acgaacuaau uauagaaaaa     3840

uaucaacaau cuauauuuaa aaaaaaggua ggcgcaauag gugauacaau cauugaaaaa     3900

agggaaaaau ucgcagagau aagucuagaa gaacaaugua aaguccugau gcaaauauuc     3960

uugaacuuuc aaaguggaau agguguagau cucacuagcg uagauggauc aaauaaauca     4020

gguguaaccu aucuaaauaa aaaaauaaug aacgcacaag aacucaaacu gauagaucaa     4080

agcguaacgg gcguuuucuc aagagaaauu gaccuguuaa caauauga                  4128


<210>  5498
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5498
augaguaaua aaucauacag uaucgguuua gacauaggga caaacagcgu uggaugggca       60

guaauaacag acgacuacaa gguaccuuca aagaaaauga aagucuuagg aaacacagau      120

aaacacuuua uaaagaaaaa ccuaauaggc gcacuacuau ucgacgaagg aacaacagca      180

gaagauagaa gauuaaaacg aacugcacgc agacgauaca cacgacgcaa aaaccgacuu      240

cgauaucugc aagaaauauu uucagaagaa augucuaaag ucgauucauc auuuuuccau      300

cguuuagacg auagcuuucu ugucccugaa gauaaacgag gcucuaaaua uccgauuuuc      360

gcaaccuuag ccgaagaaaa agaauaccac aaaaaguuuc cuacaauuua ucaucuacgc      420

aaacaccucg ccgacucaaa agagaaaacu gaucuaagac uaauauacuu agcucuggcu      480

cauaugauaa aauaccgagg gcacuuccua uacgaagaau cauucgacau uaaaaacaac      540

gauauacaga aaauauucag cgaguucauc aguauuuaug auaacacuuu ugaagaaagu      600

ucuuuaauag gccaaaacgc acaaguagaa gcaauauuca cagacaaaau aagcaaguca      660

gcaaaacgug aaagagucuu aaagcuauuu ccagaucaaa aaucaacugg ccucuucucu      720

gaguuuuuaa aacuaauauu aggaaaucaa gcagacuuca agaaacauuu cgaccuagaa      780

gaaaaagcac cacuacaauu cagcaaagac accuacgacg aagacuugga aaaccuucua      840

ggacaaaucc gagacgacuu cgcagauuua uuuuuaguug ccaagaaacu uuacgacgcu      900

auacugcuau ccggcauucu cacagucaaa gauuuaucua caaaagcacc ccucuccgcc      960

uccaugauag aacauuacga gaaucaucaa aaagaccucg ccgcacuuaa acaauuuauc     1020

caaaacaacc uacaagaaaa auaugacgaa guguuuucag accaauccaa agacggauau     1080

gcaggauaca uaaacgguaa aacaacacaa gaagccuucu acaaauauau uaaaaacuug     1140

cuuucuaaau ucgaaggugc agacuacuuc cuagacaaaa uagaaagaga ggauuucuua     1200

agaaaacaaa gaacauuuga uaacggaucu auaccacacc agauacaccu acaagaaaug     1260

aacgcaauuc ugcaucgcca aggagaacac uauccauucu uaaaagaaaa caaagaaaag     1320

auagagaaaa uauuaaucuu cagaauaccu uauuaugucg gaccccuugc cagaggaaac     1380

agagacuucg cuuggcuaac acgcaacuca gaugaagcaa uuagaccaug gaauuuugaa     1440

gaaauaguag auaaggcauc aagcgcagaa gauuuuauaa acaagaugac aaauuacgac     1500

cuuuaccuac cagaagagaa aguucuccca aaacacucgc uccuauacga aacauucgcc     1560

guauacaacg aacuaacaaa agucaaauuc auagccgaag guuugacgcg auaccaauuu     1620

cucgacaaga aacaaaagaa agacauauuu uacacuuucu ucaaagcaga agauaaacgc     1680

aaaguuacag aaaaagacau uauacacuac cuacauaccg uagauggaua cgauggaaua     1740

gaacuagagg gaauugaaaa acaauuuaac gccucacuuu cgacauacca cgauuuacuu     1800

aaaauaauaa aagacaaaga auuuauggau gacgauaaaa augaagccau acuagaaaac     1860

auuguacaca cccuuaccau auucgaagau cgcgaaauga uaaagcaacg acuugcccaa     1920

uacaacuccc uauuugacga aaaaguuaua aaggcuuuaa cacgaagaca uuacacuggc     1980

uggggaaaau uauccgcaaa auuaaucaau gggauucgcg acaagaaaag uggaaaaaca     2040

auuuuagacu acuuaauaga ugacggagaa auuaacagaa auuuuaugca auuaauacau     2100

gaugacggac uaucauucaa agaaauaaua caaaaagcac aaguauucgg aaaaacuaau     2160

gauguaaaac aaguuguaca ugaacuccca ggaucuccag ccauaaagaa aggaauacua     2220

caauccauca aaauaguaga cgaacucguu aaaguuaugg gacacgcucc ugaaucaauc     2280

guaaucgaaa uggcgagaga aaaucaaacc acagcgagag gcaagaaaaa uucgcaacaa     2340

agauauaaaa gaaucgaaga uucucucaaa aaucuugcgu ccggcuuaga cuccaacaua     2400

cucaaagaaa auccaacaga uaauguccaa uuacaaaacg acaggcuauu ucuauauuac     2460

cuacagaacg gacgagauau guacacagga aaaccauuag aaaucaacca acucucaaau     2520

uacgacauag accauauuau uccccaagcg uucauaaaag augauagcuu agacaacaga     2580

guacucacau cuuccaaaga caaucgggga aaauccgaca auguuccauc acuagaaguu     2640

gucgagaaaa ugaaagccuu uuggcaacaa cugcuagaua guaaacucau cuccgaaagg     2700

aaauucaaca aucuaacuaa agccgaaaga gagaggggcg gacuaaacga acuugacaaa     2760

guuggguuca uaaaaagaca auuaguagaa acccgacaaa uuacaaaaca cguagcacaa     2820

auauuagaua caagauuuaa caaagaaguu acugaaaaag acaaaaagaa cagaaaugug     2880

aaaauaauaa cacucaaauc caaucugguc aguaauuucc gaaaggaguu cggacuauau     2940

aaggucagag aaaucaacga cuaccaucau gcacaugacg cuuaccucaa ugccguugua     3000

gccaaagcua uucucaagaa auacccaaaa cuggaaccag aauuuguuua uggagaauac     3060

caaaaauacg acuuaaaaag auauaucagu agaucaaaag acccaaagga uauagaaaaa     3120

gcaaccgaaa aauacuucuu cuacucaaau uuauuaaauu ucuucaagga ggaaguacaa     3180

uaugccgaug gcaccauugc aaaaagggaa aauauugaau acaguaagga cacgggugaa     3240

auugcaugga acaaagagaa agacuucgca accguaaaga aaguauuauc acucccacaa     3300

gugaacauug ugaagaaaag agagguucag acagguggau ucaguaaaga auccauacuu     3360

ccaaaaggca auucagacaa acuaauacca agaaagacaa aggacaucuu augggaaacu     3420

accaaauaug ggggauuuga cucuccuguc auagcauaca gcauccucuu aauugcagau     3480

auagagaaag gaaaaucaaa agaauuaaag acaguaaaaa cgcugguugg cauaacuauc     3540

auggaaaaag caacauucga agaaaaccca auuacuuuuc uagaaaacaa aggauaucac     3600

aauguuagga aagagaacau acucugccuu ccuaaguacu cucuauucga acucgagaau     3660

ggaagacgaa gacuacuagc uagugcaaaa gaacuccaaa agggaaacga aauaguacua     3720

ccaguauauc uaacuacauu acuauaccau uccaaaaacu uacacaaauu agacgaacca     3780

ggacaccuug aauacauaca gaaacaucgg aacgaauuua aggaccugcu aaaucucgug     3840

ucagaguucu cccaaaaaua cauccucgca gaugcaaacc uggaaaagau acaaaauuua     3900

uaugcagaca acgaacaagc cgacaucgaa auucuggcua acaguuucau aaacuuacua     3960

acauucacaa cgcuaggugc cccagcagca uucaaauuuu ucggaaaaga cauagauaga     4020

aaacgcuaca cuacagucuc agaaauucua aaugcuacac ucauacacca aucaauaaca     4080

ggucucuacg aaacaagaau ugauuuaucu aaacuaggag aagacuga                  4128


<210>  5499
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5499
auggaaaaau ccuauagcau agggcuagau auaggaacaa auucaguagg augggcagua       60

auaacagaug auuauaaagu gccagccaag aaaaugaaag uauuaggaaa uacagauaag      120

aaauauauca agaaaaauuu auuaggggcu cuauuauucg acuccgguga aacagcagaa      180

gucacaagau uaaaaagaac agcacgaaga cgauauacaa gaaggaaaaa cagacuucga      240

uaccuacaag aaauauucgc uaaagaaaug acuaaagucg acgaauccuu cuuccagaga      300

cuagaagaau cauuccucac ugacgaugau aagaccuuug acucacaccc aaucuucgga      360

aauaaagcag aagaagacgc auaucaucaa aaauuuccaa ccauauauca ucuuagaaaa      420

uacuuagcug auucccaaga aaaagcugac uuaaggcugg uuuacuuagc auuagcacac      480

augauaaaau acagagguca cuucuugaua gaaggagaau uaaacgcgga aaauacagau      540

gugcaaaaac uuuuuaaugu auuugucgaa acauacgaca aaaucgucga ugaaucucau      600

uugagcgaaa uagaaguaga cgcgucauca auacucacug aaaaagucuc aaaauccaga      660

agauuggaaa accugauaaa acaauaucca acagagaaga aaaauacacu auucggaaau      720

uuaauagcau uagcccuugg acugcaaccu aauuuuaaaa caaacuucaa acucucagaa      780

gacgcgaaac uacaguuuuc aaaagacaca uacgaagaag acuuagaaga acuucugggu      840

aaaguaggug augacuacgc cgauuuauuu aucucagcua aaaaucuaua ugaugcaaua      900

cuauuaagug gaauuuuaac cguugacgau aauucaacaa aagcaccacu cucagcuucg      960

augauuaaaa gauauguuga acaccacgaa gaucuagaaa aauuaaaaga auuuauaaaa     1020

auaaacaaau uaaaacugua ccaugacaua uucaaagaca aaaccaaaaa cggauaugca     1080

ggguauauag acaacggcgu caaacaggac gaauuuuaca aauacuugaa aacgauucuu     1140

accaaaauag acgacucaga uuauuuucuu gauaaaauag aaagagauga uuuucuaaga     1200

aaacaacgaa cauucgacaa cggaucaauu ccacaucaaa uacacuuaca agaaaugcau     1260

ucaauacuac gacgacaagg agauuacuau ccauuccuua aagaaaacca agccaaaauc     1320

gagaaaauuu uaacuuucag gauaccauau uacguuggcc cacucgcaag aaaagauucg     1380

agauucgcau gggcaaauua ucacucagau gaaccaauaa ccccguggaa uuuugacgaa     1440

guuguagaua aagaaaaauc agcagaaaaa uucauaacac guaugacuuu aaacgacuua     1500

uaccuaccag aagaaaaagu ucuuccaaaa cacucacaug uuuaugaaac uuuuacagua     1560

uacaaugaau uaacaaaaau aaaauacgua aaugaacaag gagaaucauu cuucuuugau     1620

gcaaacauga aacaagaaau auucgaccac gucuucaaag aaaacagaaa agugacaaaa     1680

gcaaaacuuc ucuccuaccu gaacaaugaa uuugaagaau uuagaauaaa ugaucuuaua     1740

ggauuagaca aagacucaaa aaguuuuaau gcaucucuag guaccuacca ugaucuuaag     1800

aaaauauuag auaaaucauu ucuagacgau aaaacgaacg ggcaaauaau cgaagacauu     1860

guucuuacac uuacuuuauu ugaagacaga gauaugauuc acgaaagacu gcagaaauau     1920

ucggacuucu uuacaaguca acaacuaaag aaacuagaac gccgacauua uacaggaugg     1980

ggaagauuau cguauaaacu uauaaacggu auuagaaaua aagaaaacaa caaaacaaua     2040

cuagauuuuc uaauugauga cggacacgca aacagaaacu ucaugcaacu uauaaaugac     2100

gagagcuuau cguucaaaac aauaauucag gaggcccagg uaguaggcga ugucgacgac     2160

aucgaagcug ucguacauga cuugccaggc uccccagcga ucaagaaagg gauacuccag     2220

ucuguaaaaa uuguugacga acucgucaaa guaaugggag acaauccgga caauauagua     2280

auagaaaugg caagagaaaa ccaaaccaca ggcuacggca ggaauaaauc aaaccaaaga     2340

uuaaaacguc uacaagacuc ccuaaaagaa uuuggaagcg auauucucag caaaaagaaa     2400

ccaucuuacg ucgauagcaa aguagaaaac agccacuuac aaaacgacag auuauuccua     2460

uacuacauuc aaaacggcaa agacauguau acaggagaag aauuagacau agauagacua     2520

agcgauuacg auaucgauca cauaaucccc caagcguuca uuaaagauaa cagcauagau     2580

aacaaaguac uaaccucauc cgcuaaaaau cguggaaaau cugaugaugu cccaucaaua     2640

gaaauagucc gaaauagaag aucauacugg uauaaacucu acaaauccgg acuuauauca     2700

aagcgaaagu ucgacaaccu uacuaaagcu gagcgaggag gguugacgga agcagauaaa     2760

gcaggguuua uaaaacgaca gcuaguagag acccggcaaa uaacaaaaca uguugcucaa     2820

auauuagaug cacgauuuaa cacaaagagg gacgaaaaug acaaaguaau aagagaugua     2880

aaaguaauca cuuugaaaag caaucuugug agucaauuuc gcaaagaauu uaaauucuac     2940

aaagugagag agauaaacga cuaucaccac gcccaugaug cuuaccucaa cgcaguagua     3000

ggaacugcac uacucaagaa auaccccaaa cuaacaccgg aauuuguaua uggagaauau     3060

aagaaauaug auguucgaaa acuuauagcg aaaagcagcg augauuacag ugaaauggga     3120

aaggccacug caaaauacuu cuuuuauagu aacuuaauga acuucuucaa aacagaaguu     3180

aaauaugcug augguagagu auucgaaagg ccugauauag aaacaaacgc agauggagaa     3240

gugguaugga auaaacaaaa agacuucgau aucguccgaa aaguccucuc guauccccaa     3300

guaaacauag ucaagaaagu ugaagcccaa acaggugguu uuucuaaaga auccauccuu     3360

ucaaagggag acagugauaa acuaauaccc agaaaaacca agaagguaua cuggaacacg     3420

aagaaauacg gaggcuuuga cucuccaacc guagcauacu ccguauuagu cguagcagac     3480

auugaaaaag guaaagccaa gaaacuaaaa acuguaaaag aauuaguggg gauaucaauu     3540

auggagcgau ccuuuuucga agaaaaccca guaaguuucc uagaaaagaa aggcuaccac     3600

aauguacaag aagauaaauu aauaaaacua ccaaaauauu cuuuauucga auuugaagga     3660

ggaagaagaa gauugcucgc uagugcgaca gagcuucaaa agggaaauga aguaaugcuc     3720

ccagcacauu ugguagaacu auuauaucau gcccaucgaa ucgacucauu uaauucaaca     3780

gaacaucuca aauacguauc agaacacaag aaagaauuug aaaaaguacu aucaugugua     3840

gaaaacuucu caaaccugua uguagaugug gagaaaaauc uuucuaaagu uagagcagca     3900

gcagaaagua ugaccaacuu uucauuggaa gagaucagcg ccucauucau aaacuuacua     3960

acacuaaccg cucuuggggc cccagcugau uucaauuucc uuggagagaa aauacccaga     4020

aaaagguaca ccucuaccaa agaauguuua ucagcgacau uaauacauca auccguaacg     4080

gguuuauaug aaacccgaau cgauuuauca aaacuuggcg aggaguga                  4128


<210>  5500
<211>  4128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5500
augacaaaga uaaaagacga cuauauaguu ggacuagaua uaggaacaga uucaugcggc       60

uggguagcaa ugaauucgaa caacgacaua uuaaaauuac agggaaaaac cgcgauagga      120

aguagacuau ucgaaggagg caaaucagca gcagagagac gccuguuucg uacaacccau      180

agacguauca agagaaggag auggagauug aaauuacuag aggaguuuuu cgauccauau      240

auggcagaag ucgauccaua cuuuuuugcu agacugaaag aaucaggauu auccccauug      300

gauaaacgca aaacaguauc aagcaucgua uuuccaacau cugcggaaga caagaaauuc      360

uaugaugacu acccaacaau auaucaccua agguacaaac ucaugacaga agacgaaaag      420

uucgaccuac gagaaguaua ccuagcuaua caucauauaa uaaaguauag aggaaauuuc      480

cucuacaaua caucuguaaa agacuuuaaa gcaucaaaaa uagacguuaa aucaagcauc      540

gaaaaacuua augaauuaua cgagaaucug ggccuagacc uuaacguaga auuuaacauu      600

agcaacacag cagaaauaga aaaaguguua aaagauaaac agauauuuaa aagagacaaa      660

guaaaaaaga uagccgaacu auuugccaua aaaaccgaca auaaggaaca aagcaaaaga      720

auuaaagaua uaucaaaaca agucgcaaau gcgguuuuag gauacaaaac aagauuugac      780

acaauagcac ucaaagaaau uucgaaagau gaguuaagcg auuggaacuu caaauuauca      840

gauauagaug cagacagcaa auuugaagca cugaugggaa accuggauga aaacgaacag      900

gcaauauuac ucacaauaaa agaauuauuc aacgaaguga cccugaacgg aauagucgaa      960

gacggaaaca cauuaucaga aucuaugaua aacaaauaca acgaucaccg agaugaucug     1020

aaauugcuca aagaaguuau agaaaaccau auagaccgca aaaaggcuaa agaauuggca     1080

cuagcauaug accucuaugu aaauaauagg cacggacaac uauuacaagc aaagaaaaaa     1140

cuagguaaaa ucaagcccag aaguaaagaa gacuuuuaca aaguuguaaa uaaaaaccuc     1200

gaugauucac gagcgucuaa agaaauaaaa aaaaaaauag aacuagacuc cuucaugcca     1260

aaacaacgaa cuaacgcaaa ugggguaauc cccuaucaac uacaacaauu ggaacuugau     1320

aaaaucauag aaaaccaauc aaaauacuac ccauuccuaa aagaaauaaa ucccguauca     1380

ucucacuuaa aagaagcacc auacaaauua gacgaauuaa uaagauucag aguaccauac     1440

uauguaggac cacugauuag uccaaacgaa ucaaccaaag auauccaaac caagaaaaau     1500

caaaauuuug cguggaugau aagaaaagaa gaaggaagaa ucacacccug gaauuucgac     1560

caaaaagucg auaggauuga gagcgccaac aaauuuauua aaagaaugac aaccaaggac     1620

acguacuuau ucggagaaga cgucuuaccu gcaaauagcu ugcuuuacca gaaauucaca     1680

gucuuaaacg aguuaaauaa cauacgaaua aacggaaaaa gaaucucugu cgaccucaaa     1740

caagaaauau augaaaaccu cuucaaaaaa cacaccacug uaacaguaaa aaaacucgaa     1800

aacuacuuaa aagaaaacca caacuuaguc aaaguagaaa uuaaaggauu agcagacgaa     1860

aagaaauuca acucagguuu aacaacauac aacagauuua aaaaccucaa uauauucgac     1920

aaucaaaucg augauuuaaa auacaggaau gacuuugaaa aaaucaucga augguccaca     1980

auauuugaag auaaaucuau cuacaaagaa aaacugagga gcauugauug gcuaaaugaa     2040

aaacaaauua augcauuaag uaauaucaga cuccaaggau ggggaagauu aagcaaaaaa     2100

cucuuagccc aacuucacga ccacaacggu caaacaauaa uagaacaauu augggacucc     2160

caaaauaacu ucaugcaaau cguuacacaa gcugacuuua aagacgcgau agcaaaagcc     2220

aaucaaaacc uacuaguagc uacaucagua gaagacaucu uaaauaaugc cuauacaucc     2280

ccugcuaaua aaaaagcuau ucgacaagua auuaaggucg uugaugauau cgucaaagcg     2340

gcaucaggca aaguacccaa acaaauagcc auagaauuua caagagaugc cgaugaaaac     2400

ccaaagcgau cccaaacacg cggaucaaaa cuacaaaaag uuuacaaaga ccuauccaca     2460

gaacuagcaa guaaaacaau ugcagaagaa cuaaaugaag caaucaaaga caaaaaauua     2520

guucaagaua aauacuaccu uuauuucaug caauuaggaa gagacgcaua uacuggagaa     2580

ccaaucaaua uagacgaaau acaaaaauau gacauagacc auauccuccc acaaaguuuc     2640

aucaaagaug augcucuaga uaaccgcgua cuaguaucua gagcaguaaa caacggaaaa     2700

ucagacaaug uucccguuaa auuauuuggc aacgaaaugg ccgccaaucu aggcaugaca     2760

auaagaaaaa ugugggaaga auggaaaaau aucggacuaa uaagcaaaac uaaauacaau     2820

aacuuacuaa ccgaucccga ucacauaaau aaauacaaau cagcuggauu cauaaggcgc     2880

caauuagugg aaaccucaca aauuauaaaa cuaguaucca caauacuuca aagccgcuac     2940

cccaacacag aaauaauaac uguaaaagca aaauacaauc auuacuuaag agaaaaauuu     3000

gaccuauaua aaucacgaga aguaaacgac uaccaccacg ccauagaugc uuaccuaucu     3060

gcuaucugcg gaaaccuacu auaccaaaac uacccuaacc uacgaccauu cuuuguuuac     3120

gggcaauaua aaaaauucuc cucagacccg gacaaagaaa aagccauauu caauaaaaca     3180

aggaaguuua gcuuuauuag ucaacuacua aaaaauaaaa gcgaaaauag uaaagaaaua     3240

gccaaaaaac ucaaacgugc auaucaauuu aaauauaugc uaguuucuag ggaaacagaa     3300

acacgcgauc aagaaauguu caaaaugacu guauauccua gauucucaca cgacacagua     3360

aaagcuccua ggaacuuaau cccaaaaaaa auggguaugu cuccugauau auacggagga     3420

uacacaaaca acagcgacgc auauauggug auaguuagaa uagauaaaaa aaaaggaaca     3480

gaauauaaaa uauuagguau uccuacacga gaacuuguaa acuuaaaaaa agcagaaaaa     3540

gaagaucacu acaaaucaua cuugaaagaa auuuuaacac cuagaaucuu auacaauaaa     3600

aacgguaaaa gagacaaaaa aaucacauca uucgaaauug uaaaaucaaa aauaccguau     3660

aagcaaguaa uucaggacgg agauaagaaa uucaugcucg gauccaguac auaugucuau     3720

aaugcuaaac aacucacgcu cucaacagaa aguaugaaag caauaacaaa uaauuuugac     3780

aaagacagcg augaaaauga ugcucucaua aaagccuacg acgagauacu cgacaagguc     3840

gauaaauauc ugccacuauu ugauaucaau aaguuccgag aaaaacuaca caguggacgg     3900

gaaaaauuca uaaaacuauc auuagaagac aaaaaagaua ccauucuuaa aguauuagaa     3960

ggacugcacg acaacgcagu uaugacgaaa auuccaacua uaggacucuc cacgccauua     4020

ggauucaugc aauucccuaa ugguguaaua cuaagugaaa acgcaaaacu uaucuaucag     4080

ucacccaccg gccuauuuaa aaagagcgua aaaauuucgg aucucuga                  4128


<210>  5501
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5501
augucuaauu uugacaacca caaagacuca aucugcaaac acaaaggacu aaagaaaggg       60

aacuauuaua uuggauuaga uaucggaaca aacucaguug gaugggcagc aacaaaugaa      120

caauaugaau uacucaaauu caagucacac aaaauguggg gaucaagacu uuuugcugaa      180

gcgcaaacag caguugacac gcgaaugaaa agaucggcaa gaagaagauu acaaagacgc      240

aaauuucgau uaauauuacu ugaaucccua uuuucaaaag aaauaaaugc cguagaugaa      300

acauucuuca ugagauuaca cgaaucgaac uaucacguug aagacaaaac aaaucuuguu      360

aaauacaccc uuuucauaga caaaaauuac acagauguag acuacuauaa agaguucccg      420

acgauuuacc aucuacgcca ccaccuaaug acagaaggaa caaaagauau ccgacuucuu      480

uucuuagcaa uacaccacau ucugaaauau agaggaaacu uucuauacgg aaagaaagaa      540

uuuucaguag acggagaaau guauucagua cucaggucua cacuagaaga acucggauuu      600

ggaaguuacg uagaagauga aaacguacuu caaucaauag caaaucuagu acuagaucga      660

ggaauugccc guucugaaaa agucaaacaa auugagccac uaauugcgca aggcaagaaa      720

cuaagcucug uagagaaaaa ucgauuaaaa gcaugggcuu accucauaau gggcuuaaaa      780

gcgaacuugg uuaacuuauu uacagacgaa gacguugaag uaguauuacc agaagagaaa      840

gaauuuaaau caaucaauuu acaugaggga auauacgaag auguacgaga cgcuuacgaa      900

gaugcuuggg gcgaccgauu ugccauuguc gaccaaugua aaguccuaca cgacaucauu      960

guuauaucga gcauuauuaa accagggcaa ucccuaagcg aagcaaaaau cgaaucauac     1020

aaccaacaua aagaagacuu aguacuacuu aaaagcguac uaaaagaaga uaagaaacua     1080

uaugacgaua uguuuguugu agauauagau aaaggaacua acuacgugaa auacaucaaa     1140

caaggaguca acgguaacac aucuaccacu caagaagcgu uuuauaaaua uguaagaaaa     1200

guagucucag uucucccaga aaccgaaaau acucgcugua uaguccaaaa auuagaggaa     1260

ggaacauucc uuccacucca acgaauuagu aaaaacggag ucguaccuua ccaauuacau     1320

aaagaagaac ucgucagaau uuuagaaaaa gcaaaauuaa acuuccccuu ucugaaugag     1380

guagaagaug gucucacagu agcggacaaa aucauuagca uacucgaguu caaaauaccc     1440

uauuacguag gaccucuaaa cccaacacac uccauagaga auggagguuu cgccugggua     1500

guacgaaaag aagcagguca aguacuucca uggaauuuug aacaaaaagu ugauguacaa     1560

gcaucagccg ucaaauuuau agaaaaccug acaaacaaau guacauaucu aaucgaccaa     1620

gauguccuac caaaauauag ccuccuauau gcggaauuuu cucuacuaaa cgaguuaaac     1680

aauauaaggg uaaacgauaa accuuuacca gcagacguca aacaggcuuu cauaagagau     1740

cacuucauaa gucuacaaga cuccaaaacc gcaaccaaga aaguaguagc uaaauaucua     1800

guagacaaca acuacguauc acaaccgcca auaauaacag gcauggauca acaaauagca     1860

acaaaacuaa caagccaccg cgauaugaca cggauacucg gagaacauuu ugacuacgcu     1920

auggccgaag auauaaucag auacauuaca aucuucggag aauccaaaga uauguuaaga     1980

caaguccuua aaaauuauua uggagaucgu cuaacaacgg aacaaauuaa gaaacuaggg     2040

aaauugacau auacaggcug gggucgacuu agcaagaaau uccuuucuga cauauggggc     2100

augaagaaaa augacgauga cgcugaagca gguacaauca uagagcacau gagagaaggc     2160

caagagaacc ugaugcaacu gcucucaucu aguuauucau ucauagaugu aguccaggaa     2220

uacguaacca acguucuagg ggcgaaagag aaaaccgcau uugaaauguu agaagaccua     2280

ucacucagcc caaugguuaa aagaucuguu uggcagacau uaagaauacu cgaugaaauu     2340

guaucaauca gaaaagaacu acccaagaaa aucuuuguag aaguagcaag aacgaacaaa     2400

gccgauaaga aacgaaccca gucucgaaaa gcguuacucg aagaacuuua cgaacaaauc     2460

aaagacgaag acguucaugu auuacaacaa gaacucgaau caaaagaaga cucagcucua     2520

aacagcaaga aacuguaccu auauuuuaca caaaugggaa gauguaugua cucuggacgc     2580

cguauagaua uacacgaacu auucacagac acuuacgaca uagaucauau auacccaaga     2640

ucgcuaacaa aagacgauuc auggcauaac auuguacuug uugaaaaaca auugaacgcc     2700

gagaaaacag aucgauuucc aauauuaagc gcaauacaau caaaacaaaa aggauucugg     2760

aaacuauuau gggauagagg auucauauca gaaaagaaau augacagacu uguaagaacc     2820

acugacuuaa ccgacgaaga guuaaaucaa uucauaaauc gacaaauagu cagcacaaau     2880

cagaguguca aagccgugac cacgcucuua caccagcuuu acccugaaac agagguugua     2940

uuuucgaaag cagaaaacau auccgauuuu agaagagacu auggauucgu aaaaguacgu     3000

agcauaaacc accaucauca ugcgaaagac gccuacuuaa auauaguugu aggaaaugug     3060

uaucaugaaa aauuuacgaa aaacuuuuug ucuuuugcaa aacaaaaagg cacucauaga     3120

acguacaauu uaacaaaaau guuugaauau gagauaaaac uaacagagga aucgaagaaa     3180

guaauauggg auccaaauau acauauggac auaguaaaga aaaugaugaa aaguaacgau     3240

guuaggguaa cacaaagagc aguuccgcaa aaaggcgggc uaagcgacga aacaauauac     3300

aagaaaggca uagcaaaaag aggaguauau cuaccacuaa aaagcucaga cgaaagaguc     3360

agagauguag aaaaguaugg agguagaaca ucaauaacaa uaagcgaaua cauuauacua     3420

aacauccgcg auacauccga ccaagauaga auagaaauau uuagccuacc uuuauacaua     3480

gcaggcaaga aagacaucca caaagccauu gaagaagccu aucuaaguac augcaagaaa     3540

ccagcaucag ucguagacau ccaaauacua uauagaggau uguauauagg auccaaaguc     3600

aacauaaacg gauucacaua cuauaugggu ggaaaaacag gaucaaacgu cagcguggac     3660

ucagccguug acguaguaau ggauaucaca ucaucaguuu acauaaaacu ccuugaaaaa     3720

guugcaauca agaaaaucga aaauucaaaa acauuuagga ucgaaaauau aaaaaccuca     3780

uacaaccaca aagaagucac ugucucuaag gaacaaaaua ucgcucuaua ccaagaacuc     3840

cucaagaaau uagacacucc uuuauucuca aaaaugaaag guaacaaacu agaagaguuc     3900

agaaccguag gcuuugacac auuuauagca cuaucauuag aagaccaagc aucaguccua     3960

cuagaagucc uaaacguacu aacaaauuuu aaaacaaaac gagacauuac uaaaauaggu     4020

augaccucau cacgaaguac guuggggauu aauauaucua acacggaaca auucucagua     4080

aucaccacuu caauaacugg acuauaugaa caagaaauaa aaauaaaaug a              4131


<210>  5502
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5502
augaccacaa aaaacagaga aaaaaauaac aaauuucaua aagcaucccc cuuauauaca       60

uuaggccuag auauagguac aaccucaguc ggauucacag caauagauga agcccaaaga      120

ccaaugagag cuaaaggcaa aaccguuauu ggaguacgac ucuuccaaga aggacaauca      180

gcgguaggaa gaaaaaacuu uagaacuaca cgccgacgac uaaaaagacg aagaagacga      240

cugaauuuac ucaaacaauu uuucucagcc ccauuaagga agauagaucc uacauuuuuc      300

aaacgacuca aagcaucauc acuaucuccu gccgauaaaa gaagauaccu ugcaccagga      360

cuauucccua augguggcga uauuaaauuc uaccaaaaau accccaccau auaucacuua      420

aggcuagcuc ugaugacaga aaaaagaccc uuugauuuac gcgaagucua ucuagcuauc      480

caccacauag uuaaauauag gggaaacuuu cuaaacccac uacccguuuc ugcauuuaga      540

ggacgaaacc aaagauuuga ggaccaauuc agcgaacuua aucaacuaua uaaaucacuc      600

gaaggggaau cacccuuuca auuugaugaa aaaauauucu cagaacuaga caaacaacua      660

uuaguaaacg augguagaaa aauugauagg caaaaacaac ucgcuaaacg cguauuuaua      720

aaaacgaaug aaaagcgcau agauucccac caucaaaaaa uagcuuccgc uuuuucuaaa      780

gcagucuuug gauauaaguu cagaccagau guacuguuaa acucagaucc caacgaaagc      840

auagaugaau uuaucaaguu uggagaugaa gauuucgacg acaaacuagu aauauuacgc      900

gaaucacuaa acgcaacuca aaaagaaauu uuagcaguca cacaaagaau cuauucccaa      960

auuauucuua acgaaauagu uccaucucgc gagacguuaa gcgaggcaau gguaucuaga     1020

uaccaaaaac accauucaga uuuacaacuu uuaaccgaag cauguaaaaa gaugacuaaa     1080

gcaaaagcgg ccagaauuca agcagcauac acugacuaua ucgggaaagg ggacggccac     1140

aaacugguuc acgaucaauu uguagcacuc auaaaaaaaa acuuagaucc uagugaagaa     1200

ggaagggauu uagcagaaag guuagcgcaa gaugacuuca ugcccuuaca acgcacgccc     1260

cugaacagag uuauacccca ucaacuccaa caagaagaac ucgaccgaau cauagaaaau     1320

caaggugaau auuacccauu ccuuaaaaca cuuaauccaa accgaucucg agcuggaguc     1380

gcgaacuauc aacucgacga acucguauca uuccgaaucc cauacuaugu ugguccacua     1440

aucacggcaa cugaccaacu acacacaucc ggagcaaaau ucgcuuggau gacaagacgc     1500

gccgcaggug aaaucacccc cuggaacuuc gaucaaaaag uggaucggcu agcaucagcu     1560

aaucaauuua uaagacaaaa uaugccaaaa gacaaccauc ugcuagaagc agauguauua     1620

ccggcaaacu cccuaacaua ucaaagauau cucguccuag acgaguuaaa ugcaguuaaa     1680

guaaaccaca agucgcucac cgucucgcaa aagcagcgaa ucuuuaacga ccuauuuaaa     1740

cgccaaagaa ccguaacagu acucaaccua caaaacuacu uaaaaucaau agaaaaacua     1800

ccaacccucc cagucaucac uggagucucc gacucagaaa gauuuaaaac uacacuauca     1860

acauaucucg acuucaaaaa uaucuucggu ggaaaaguug augacucuag ucaaaaggaa     1920

aaauucgaaa aaauuaucuu auacgcuaca auauuugaag aucaugcagu auauaaagca     1980

aagcuagauc aaauagacug gcuuggacaa caacaaaaaa cugauuuacu aucaaaaaac     2040

uaccaagguu ggggacaacu auccgaaaaa cuacuccugg guuuaaaaaa ccaggcaggg     2100

caaagcauau uagaugaacu guggcaaacu aauaaaagau ucagagaaau aaugaacgaa     2160

gcacacuuug cuaaacaaau uauggcaucc aacgcagccc uauuacguaa aaaagguuua     2220

gaugaugucu uaucaacugc auuucuauca ccacagaaua aaaaagcaau aagacaaacu     2280

guggaaguug ucgacgacau aguaaaagcu auugguuguc caccacagaa aauaucucuc     2340

cacuucagua aagccccagc cgacgcaaua cgccaugaga gagauaacgc aaacauaauc     2400

cuacgaacuu accgaaacuu accugccaaa augguagaug accaacuuaa ggcagaacua     2460

caagcaacag cagaaaaaca ucaaguauug ucagauaaau uguauuuaua uuuuacacag     2520

cugggacgag aucuauauac aggagaaaug cuuaaaauga gcgaacuaga aaacgacaug     2580

auugaucaca ucaugcccca aacauucgca uacgaugacu cugucaacaa ucgcguacuu     2640

guaucgcaaa aucauucuuc acaacuagga aaaacauccg gugaaaaauc ugacgcuaaa     2700

augauccgau ucuggcaagu auuacgcgac aauaaacuga ucagugaaca gaaauaucaa     2760

aaccuuacuc ucgacagcga ucaccucuca aaaagcacca gggaacaacu cuuaaaccga     2820

uacuuaguag agaaaaaccc aauaacacaa ugggucgcau caguucuuac agaaaaguau     2880

ggagauaaug gcaccagggu auuaaccaua aauccgcgaa ugguauacca aaugagaaga     2940

caauucaauc uaguuaaucu uccugagguu aacgacuaug accauggacu cgaugcauac     3000

uuagcagcau ucaucggaaa uuauguagau cgaagauacc cuagacucag auccuauuuu     3060

guauacggag acuucucauc aucaaaagaa auagucuuuc gacucaacag guucaauuuc     3120

uuauucgacc ucgggcaagc agaacccaau gagcuaacgg acugggaaac aggggaaaug     3180

auaugggaca aagcugcagc aaauucaguc uuacgagaug uauaccaaua caaauuuaug     3240

cugauaucac aagcaguuua cucccgacaa ggagcaaugu ucaaucaaac uauauauccu     3300

gcuucacaag cugccgggcg ucaacuaauu cccguaaaaa aaaacagagc cacuaaauug     3360

uacggcgguu acagcggaaa cguagaugcc uauuuagcaa uuguucgauu agguggacca     3420

aaaucagacc aguaucgcgu aguaggaauc ccaacaagaa ugguaggaag guuacaaucu     3480

gccaugaagc aaggugcuga cacauaugca aauacauuac acaaaauauu gcaaccccaa     3540

uuuaccaaaa ggaaaaaaaa ucgccaaacu gguuacuaug aagagacaac ugagccauuc     3600

gcaguaaucu uggauaaagu auacuacaga caauuaauaa uagacggaca gcaaaaauuu     3660

auguuaggca guucuacuua ucaauucaac gcaaagcagc uaguauuauc uucacgcgca     3720

agacaaauac ucucaggcga agaacgguua acagacgaaa acgggagcca agaccugauc     3780

gacguauacg augagauccu aacgaaaaua gacagauacu ucgaauuaua cgaccaaaga     3840

cacuuuagac aaucacucca ugauggaaga aaacaguuca uaucccuccc uauccauaac     3900

guuuacagag ccaaucgacu uaucgagcac ggaaaauacg agacauuaag ccaaauccua     3960

aacggacuuc augcaaacac caugaugaca aauuuaagau aucuuggaau aucgucaccu     4020

uuugguaaau uucaaucucc caacggcaua aaacucucaa aagaugccgu guuaguauac     4080

caaucaccau cagggcucuu cgaaagaaga gugagacuaa gagacuuaug a              4131


<210>  5503
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5503
augaacaaua aaccauacuc aauaggacuu gacauaggaa caaauucagu cggaugggca       60

gugauaacgg augacuacaa aguaccaagc aagaaaauga aaguacuugg aaauaccgac      120

aaacacuuca ucaagaaaaa cuuauuaggu gcacuuuuau uugaugaagg aacaacagcc      180

gaagacagac gacuaaaacg aacugcacga agacgcuaca caagacgcaa aaauagacuc      240

cgauaucuac aagaaauuuu cacugaagaa augagcaaag uagauauauc auuuuuccac      300

cgucuagacg auucauuccu ggugccagaa gauaaaagag gaucgaaaua uccaauuuuc      360

gcaacccuug aagaagaaaa ggaauaccac aaaaauuucc cgacgauaua ccaucugcgc      420

aagcaccuug cagacucgaa agaaaaagca gacuucagau ugauauaccu ugcauuagcc      480

cauauuauaa aauauagggg acauuuccuu uacgaagaau ccuucgacau caaaaauaac      540

gauauucaga aaauauuuaa cgaauuuaua ucaauauacg auaauaccuu ugaaggaucc      600

ucacuaaacg gccaaaaugc gcaaguugaa gccauauuca cggauaaaau aucuaaauca      660

gcaaaaagag aacgcguucu uaaacuauuu ccagacgaaa aauccacagg ccucuuuucu      720

gaguucuuaa aacuaauagu cggcaaucaa gcagauuuua agaaacauuu cgaucuugaa      780

gaaaaagcac cccuucaauu cucaaaagau acauaugacg aagaccuaga aaaccucuua      840

guacaaauug gugacgacuu ugcggaccua uuucuaguag cuaagaaacu uuacgaugcc      900

auacuacuua guggcauuuu aacaguaacu gaucccagua cgaaagcccc acuuuccgcc      960

aguaugauag acagauauga aaaucaccaa aaagauuuag cagcucuaaa acaauucaua     1020

aaaaccaacc uucccgaaaa auacgaugaa guauucucag accaaucaaa agacggcuau     1080

gcaggauaca uugauggaaa aacaacacaa gaagcauucu auaaauacau uaaaaacuua     1140

uuaucaaaac ucgaaggugc agacuauuuu cuugacaaaa uagaacgaga agacuuccuc     1200

agaaaacaac gcacauuuga caacgguuca auaccccauc aaauacauuu acaggaaaug     1260

aaugcuauaa uaagacgaca aggagaacac uaucccuuuc uccaagaaaa caaagagaaa     1320

auagagaaaa uuuuaacauu cagaauaccc uacuauguag gaccccuagc ucgaggaaau     1380

agagacuucg cuuggcuuac aagaaauagu gaucaagcca uuagaccaug gaacuucgaa     1440

gaaauagugg auaaagcaag aucagcagag gauuuuauua auaaaaugac aaauuaugau     1500

uuauaccuac cagaagaaaa aguacuacca aaacacucac uacuuuauga aacuuucgca     1560

gucuacaaug aacucacgaa aguaaaauuu aucgccgaag gacuuagaga cuaucaauuc     1620

cuugacagug gacaaaaaca acaaauaguu acccaauuau uuaaagagaa acguaaaguc     1680

acagagaaag acaucauaca auaccugcau aacguugacu ccuaugacgg aauagaauua     1740

aaaggaaucg aaaaacaauu uaacgcaucg cuaucaacau accaugaucu acucaaaaua     1800

auaaaagaca aagaguucau ggaugauucc aaaaacgaag ccauccuuga aaauaucgua     1860

cacacucuaa caauuuucga agaccgcgaa augaucaaac aaagauuagc acauuacgca     1920

agcauauucg acgaaaaagu uauaaaagcu cucacuagac gacauuauac aggauggggu     1980

aaauuaagug ccaaacugau uaacggaaua uacgauaaac aaagcaagaa aaccauacua     2040

gauuaucuaa uugacgaugg cgaaauaaac cgaaauuuca ugcaacuaau caacgaugau     2100

ggacuaucau uuaaagaaau aauacagaaa gcacaaguag uaggaaaaac uaaugacguc     2160

aaacaaguag uccaagagcu uccuggaagc ccugcaauaa agaaaggaau ccuccaaagu     2220

aucaaacucg ucgacgaauu agugaaagua auggggcaug caccagaaag uauaguaaua     2280

gaaauggcac gcgaaaauca aaccacugcg agaggcaaga aaaauagcca acaacguuau     2340

aaaagaauag aagacgcccu uaaaaaucuu gcacauggcc uagacucgaa cauccuaaaa     2400

gaacacccua ccgauaacau ucaacuccaa aacgaccgac uuuuucuaua cuaccuacag     2460

aauggaaaag acauguauac uggaaaaagc cucgacauaa accaauuguc uucaugcgau     2520

auagaucaua ucauuccgca agcauucaua aaagaugaca gccuugacaa ccgaguauua     2580

accucaucaa aagacaauag aggaaaaagu gacaauguac caucacuaga aauuguucaa     2640

aaacgaaaag cguuuuggca acaguuacua gacucaaaau uaauaucaga aagaaaauuc     2700

aacaauuuaa caaaagcuga aagaggugga cucgaugaaa gagauaaagu aggauuuaua     2760

aaaagacagc uagucgaaac ccgacaaaua acgaaacacg uagcccaaau acuagacgca     2820

cguuucaaca ccgaaguaac agaaaaagac aagaaagaua gaucagugaa aauaauuacg     2880

cuaaaaagca aucuaguuag caacuuuagg aaagaguucc gcuuauauaa aguacgagaa     2940

auaaaugacu aucaccacgc ccaugauccu uacuuaaacg ccgucguagc caaagcaaua     3000

cuaaagaaau acccaaaacu cgaacccgag uucguauaug gugauuacca gaaauaugac     3060

uuaaaacguu auauaucaag aacaaaagac cccaaagagg ucgaaaaagc aacagaaaaa     3120

uauuucuucu acucaaacuu acuaaauuuc uucaaagaag aaguucacua cgccgacgga     3180

accaucguaa aaagagaaaa cauugaauac agcaaagaca caggagaaau ugcauggaau     3240

aaagaaaaag acuucgcaac caucaagaaa guccucucau uaccucaagu aaauauaguu     3300

aagaaaacag aagaacaaac aguuggacaa aacgggggcu uauuugacaa uaauauagua     3360

agcaaaaaga aagucguuga cgcuagcaaa cuaaccccaa uaaaaagugg acuuucccca     3420

gaaaaguacg gaggauaugc uagaccuaca aucgcauacu ccguacucgu gauagccgac     3480

aucgaaaaag ggaaggccaa gaaacugaaa agaaucaagg aaaugguagg aauaacagua     3540

caagauaaga agaaauuuga agcaaaccca auugcauauu uagaagaaug uggcuauaaa     3600

aacauaaacc cgaaccuaau cauuaaacua ccaaaguaca gccuuuuuga auuuaauaau     3660

ggacaaagac ggcuucucgc gucauccauu gaacugcaaa aaggaaauga auuaaucgua     3720

cccuaucacu ucacagccuu auuauaucac gcgcaaagga uaaauaaaau cagcgaaccg     3780

auucauaagc aauacgugga aacucaucaa agugaguuca aagaauuacu aaccgcaauc     3840

auuucccuaa gcaagaaaua cauucaaaaa ccaaacguag aaucacucuu acaacaagcc     3900

uucgaucaaa gugauaaaga uauauaccaa cuaucugaau cuuuuaucag cuuauuaaaa     3960

cucaucagcu ucggugcacc cggcacauuc aaauuccucg gaguagaaau aagccaaagu     4020

aauguucguu aucaaucagu aucuagcugu uuuaacgcaa cacuuauaca ucaaaguauu     4080

acaggacuau augaaacuag aauagacuua uccaaacuag gagaagauug a              4131


<210>  5504
<211>  4131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5504
auggacaaga aauacggaau aggauuagac aucggaaccu cguccauagg auggacuguu       60

guagacaaau auggacauau cauaagaguu aaaggccaaa cuggaaucgg aguacgauua      120

uucagagaag gucaaacugc agcagaaaga agaacauuua gaacaaccag aagacguuua      180

ucuagacgua gauggcggcu gagacuacua cgagaaauau ucgaugaacc aauaucagcu      240

auagacgaaa auuucuuugc ucgucaaaag gcuucaucac ucgcaccagg ugaugcacga      300

uaucaacaag cauuccaacu cuuuaaugau agaacugauc aagccuuuua cuguaaauau      360

cccaccauau accaucucag acaagcacuu augacugaaa guagacaauu ugacaucagg      420

gaaauuuacu uagcacuaca ccaaauaaua aaguaucgcg ggcacuuuuu gcuaggagga      480

gcggcaaaag auuuucaauc agacaagcuc gauuuagaag cguacuucac aacaaucaac      540

gcucaacuag cagggaucua cucagacggu agcgugcaau uacccacacu agacuggaac      600

caggccuguc aagagcuuac agauacauca agaacacagu cagaccguaa aaauuggcuu      660

gucaaagaau uaaagagcua cggagauuca caauuaaaac cgauauacac ucaaaucgcc      720

aacgcgauac uaggauucaa aacagacuua gccaaaauaa ugggcaucaa cgacccagaa      780

aaagcacuua guuuuaaccu cgaugcacua gaugaucaua gggaugacgu cgcggaauua      840

uuaacggaug agcaaauacc ucuagugaac uuauuagcua aacuacauac ugcauuggaa      900

cuugcagcaa uccuaccaga cggucaaaca cuaagcggaa aaaugguaga gauauaugaa      960

acccacagaa aacaauuaaa aacauacaaa gccuucauaa gacaauuacc ggaccgccaa     1020

cagcgacuag cucuaaaaca ggcauaugac cauuauaucg aucaaggucu aaauagagag     1080

gcauucuauu cugaagucaa agcuauacuc gaaaaacaaa aacccuuaac cgcgacaggu     1140

caagcaauga agaaagcuau ggaucaaaau gcuuucuuac uaaaacaacg aacaaaaacc     1200

aauauagcaa ucccuuauca agugcaacag caagaaaugg accaaauaau cgcuaaccaa     1260

ucgaaauacu auccauggcu agcaacaccu aauccuguaa aaucaagaag aguaauagca     1320

ccauacaaac ucgaugaauu aguaugcuuc cgugucaauu acuacguagg accauuagua     1380

caauccaagc caggagaagc uacaaaagaa gcaaaauuug ccuggaugac ccguaaagau     1440

ccacaaucaa cuguagcgau cacuccuugg aacuuugauc aacaagugga cagacaagcu     1500

agcgcaaaug cguucauuca acgaaugaaa acuacagaua cauaccuacu uggagaggau     1560

guuaucccca aaaacucuuu auuauaccaa aaguaugagg uuuuaaauga acuuaauaau     1620

guuagaguua auggagacug guuagacccc caaagaaagc aauaucuauu caagaaauua     1680

uuuauggauc aaccacaaag uguaguuaca guuaaagcau uacaaaagac ucuaguaguu     1740

auuggagcau gucccacacu accaacagua gaaggauuag ccaacccaaa gagauuccua     1800

ucccaauuga caacauacca cgauuuuugu aaacuacuac cggacgauau ccaaagagca     1860

gauagacaag cagauaucga aaagauaaua aauuggagua caaucuucga agauacugcu     1920

aucuuuaaag aaaaauuacg agacauaaaa ugguuauccg cagaucaaau aagacgauua     1980

aguucgcguc caagauauag agguugggga cgcuucagug caaaacucuu guguggacuu     2040

cacgaccgac aagguaauac cguaauggac gaauuauggc agaccucacg aaacuuuaug     2100

caaaucauca gacaauccga cuuucagaca gcuauagcag caguaaauca acaagacuug     2160

ggacgacaag aucuccagag aguaauugac gacuuauaua ccucaccaca aaauaagaaa     2220

gcuauaagac aaguccuagc cguuguaaca gauauccaaa aagcaaugca uggaaaggca     2280

ccaucuuggc uauuugugga aguagccaaa ggcgcacaga aaaacccuca aagaacgcaa     2340

agccgacaau cuaaacuagc agaagcauac cgagaauccg cucgagagcu aguagaugaa     2400

gcaguaaagg aacaauuacu aacccuacca gaugaccgcc aaacauuuag cgacaggcua     2460

guucuauauu uccaacaaaa cggaagagac aucuacaccg gagaaccacu cgauuucgaa     2520

cgacucucaa auuaucacau agaccacauu aucccacaag caguaguaaa ggaugauagu     2580

uuagacaacc gaguacuuac aaguaaccaa gcaaaccuug aaaaaggagc ucauguuccc     2640

acaauauuua gagacaaaca agagccagug uggcaagagc uuuaucgcgu cggacucauu     2700

agccauagaa aacuucgaaa ccuacuaacc acaccagaug acuuagacga uagagggaga     2760

gcaacgggau ucaucgccag gcagcuaguc gaaacacgac aagucaucaa guuaguuaug     2820

gaaguacucu cucaauacua ugacacaacg gaaacugaau uaauaucaau aaaagcagga     2880

cuuucccauc aauuucgaca agaauuaaac cuacccaaac ugagagcacu aaaugauuac     2940

caccaugccc aagacgccua ccuagcugca cgcauaggua cuuacuuacu ucaacgauau     3000

ccaaauuuaa agcguuucuu uguauacgga gauuucaaga aaacccacuu ugaucuacaa     3060

cgaagauuua acuucauagg agguaugauc ucucgcgaaa aacaaccgua caaccuuauc     3120

aaaaacccug uaacccacca aguaauaugg gacgaagaaa uggagaucgg cgauugccaa     3180

aagaaaaauc ccgaacaacc aaaacacgau gcuacaguaa aaaugaugaa ucuaaccaaa     3240

auaaugaauu uaaaacacau ccucguaacc cacgaggucu acgaaaauca uggggcucuu     3300

uuuaaucaaa ccauauaccc ugcaagccaa gcacacaaaa agaaauuaau accacagaaa     3360

accgaaaaac cuauagaacu guacggagga cauucaagcg aacaagcagg auauauggcg     3420

aucauaaggc uucuuggcaa gagaccugga uacaaaguua ugagaguucc aguucgauau     3480

guuucacaac uacaacacca agaugucgca gaacaaaaga aauuacuaua caagauauuu     3540

gucucagaac uaacaucaag aaauaaagua aagccuuuug aaguaguugu accucacguc     3600

uguuuaaacc aacccguaga agacaugaua aaaggacaau ggcaccgauu cgcacuaagg     3660

acaaauggau auuaucaaaa ccaacaacaa cucauacuaa gucucguggc ucaacgaaca     3720

cuggcagguu acaaacccaa agacgacgaa cuaauggcag uauaucauga aauuugcaaa     3780

caaguaaaau auuauuuucc ccuauacaaa augaauaaau uccaaucgag auuauucgca     3840

gcagaagaac uauugcagaa aagaucaacc gucaauucau uuaguauaac gauaggaaag     3900

aacggaaaag aaaacaaaac auugaauuua gguaaacgug aacugcuauu aagauuacuu     3960

gggggcuuac acgcgaacgc aacucgagua gauuugaaag auauaggaug uacaggccaa     4020

uuuggccaac uacaaguaaa gaauggcauc aaacuaacag aacaagcagu cuuaauacau     4080

gagucgccaa cagguuuauu ccaccggcga guaagauuaa augaucuuug a              4131


<210>  5505
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5505
augacaaaga aaaacuauuc cauaggacuc gacauaggaa cgaauagcgu aggaugggca       60

guaauuacag augacuauaa agucccagcc aagaaaauga aaguguuagg caacaccgau      120

aagaaauaca uuaagaaaaa ccuauuagga gcacuacuuu uugacucagg cgaaacagca      180

gaagccacac gauuaaaacg aacagcuaga agaagauaca caagacguaa aaauagacua      240

agauaucuac aagaaauauu cgcagaagaa augacuaaag uugacgagag uuucuuuuac      300

agauuagaug aauccuuccu aacaacagau gaaaaagacu uugaaagaca uccaauauuc      360

ggaaauaaag cggaugagau uaaauaucau caagaguucc cuacuauaua ccaucugcgu      420

aaacauuuag cugacucauc cgaaaaagca gaccuaagau uaguauaccu agcacuagcg      480

cacaugauaa aauuccgagg gcacuuucua auagaaggcg aacugaaugc cgaaaacaca      540

gauguacaga aaauauucgc agauuucguc ggaguauacg aucgaacuuu ugaugauuca      600

caccuaagcg aaaucacagu ggaugcagca ucaaucuuaa cagagaaaau aaguaaaagu      660

cgacgacuag aaaaucuaau caaauauuac ccuacagaaa agaaaaacac acucuucggu      720

aauuuaauag cacuagcauu aggacuacaa ccaaauuuua aaaugaacuu uaaauuaucc      780

gaagacgcca aauuacaauu uucaaaagau ucauacaacg aagaccugga agaacucuua      840

gggaaaauag gagacgacua ugcagaucuc uucaccagug cgaaaaaucu uuacgaugcc      900

auauugcuca gcggaaucuu aacaguggau gacaacucaa caaaagcacc acucagcgcc      960

ucaaugauaa aacgauacgc cgaacaucac gaagaucucg aaaaauugaa agaguucaua     1020

aaagcaaaua aaucugaacu cuaccacgau auauuuaaag augaaaccaa aaauggauau     1080

gcaggauaca uugaaaacgg aguaaaacaa gaugaauuuu auaaauaucu caaaaauacc     1140

cuaaguaaaa ucgcuggguc cgacuacuuu cuagacaaaa uugagaggga agacuuccuc     1200

cgcaaacaaa gaacauuuga uaauggaucc aucccacacc aaauacaccu ccaagaaaug     1260

cacgcaauac ugcgacgcca aggagauuau uauccauucc ugaaagaaaa ucaagaccga     1320

auagagaaaa uccucacguu uagaauccca uacuacguag ggccauuagc acgaaaagac     1380

aguagguucu ccugggcaga auaccacuca gacgagaaaa uaacuccgug gaacuuugau     1440

aaaguaaucg acaaagaaaa aucugcagaa aaauucauca caagaaugac acuuaacgau     1500

cuauaccuac cagaagaaaa aguucuacca aaacacaguc acgucuauga aacguacgca     1560

gucuacaacg aauuaacgaa aauaaaauac guaaacgaac aaggaaaaga uaguuucuuc     1620

gauucaaaca ugaaacaaga aauauuugac cacgucuuua aagaaaauag aaaagucacu     1680

aaagaaaaac uauuaaauua ccuuaauaaa gaguucccag aauauagaau aaaagauuua     1740

aucggacuug acaaagaaaa uaaaucuuuu aacgcaucau uaggaacaua ccacgaccua     1800

aagaaaauuc uagacaaagc auuucuugau gauaaaguca augaagaagu cauagaagau     1860

auaauuaaaa cuuugacccu auuugaagau aaagauauga uacacgaaag acuacaaaaa     1920

uauuccgaua uauuuacugc agaucaauua aagaaauuag aacgaagaca uuacacuggu     1980

uggggcagac ucagcuauaa acuaauaaac ggaauaagaa auaaagaaaa uaauaaaaca     2040

auacuagauu acuuaauuga cgacggaagc gcuaauagga auuucaugca acuaaucaau     2100

gacgacacau ugccauucaa acaaauaaua cagaaaucuc aaguugucgg agacguagac     2160

gacauagaag caguagugca ugaccuacca gguucacccg caaucaagaa agggauacuu     2220

caaaguguaa aaauaguuga cgaauuagua aaagugaugg gagacaaucc cgacaacaua     2280

guuauugaaa uggcucgcga aaaccaaacc acaaaucgug ggagauccca aucacaacaa     2340

agacuuaaga aacuacaaaa cucauuaaaa gaacucggau ccaacauucu aaaugaagaa     2400

aaaccgaguu acauagaaga uaaagucgaa aauagccacu uacaaaacga ccagcucuuu     2460

cuuuacuaca uccaaaacgg aaaagauaug uauacaggcg augaacuuga caucgaccau     2520

cuaagugauu augacauaga ccacauaauc ccacaagccu uuauaaaaga cgauucaaua     2580

gauaacagag uacuaaccag uuccgccaaa aauagaggaa aaucugacga uguccccagu     2640

cuagauauug uuagggcaag aaaagcagaa ugggucaggc uauauaaaag cggauuaauc     2700

agcaaaagaa aauuugacaa uuuaacuaaa gcagaaagag gcggcuuaac agaagcugac     2760

aaagcaggau uuauaaaaag acaauuaguu gaaaccagac aaauaacaaa acacgucgcc     2820

caaauccuug augcaagguu uaacacugaa uccgacgaaa acgauaaagu uauuagagac     2880

gucaaaguua uaacauuaaa aucaaaccua gucucccagu ucagaaaaga cuuugaguuc     2940

uauaaaguca gagaaauaaa ugauuaccau caugcacaug acgcguacuu aaaugcagua     3000

guuggaacgg cauuacuaaa gaaauacccc aaauuagcau cugaguucgu cuauggagaa     3060

uacaaaaagu augauguaca uaaacuaauu gcgaaaagcu cugaugacca cucagaaaug     3120

ggaaaagcua cggcaaaaua cuucuuuuac aguaaucuaa ugaacuuuuu caaacgagua     3180

auccgauacu ccaacggaaa agugaucgua aggccagucg uagaauauuc aaaagacacc     3240

gaggacauag cgugggacaa gaaaaguaac uuuaggacaa uauguaaagu acucuccuau     3300

ccucaaguca auauugucaa gaaaguugaa acacaaacag gaggcuucag caaagaaagu     3360

auccuaccaa agggggacuc agacaaacua auaccaagaa aaaccaagaa agcauacugg     3420

gauacaaaga aauauggcgg cuuugauuca cccacgguug cuuacagcgu cuucguaguc     3480

gccgacguug aaaaagguaa agcaaagaaa uuaaaaacgg ucaaagaacu uguaggcaua     3540

ucuauuaugg agcgaucauu uuucgaagag aacccaguag aguucuuaga aaacaaagga     3600

uaucacaaca ucagagaaga uaaacuaauu aaacucccaa aauauagccu guuugaauuu     3660

gagggaggaa aaagaagauu auuagcgucc gccucggaau uacagaaagg caaugaaaug     3720

guaauaccag gacauuuagu aaaacuucua uaucacgcac aacgaauaaa uucuuucaau     3780

aguacgaaau auuuagauua cguaucagca cauaagaaag aguucgaaaa aguuuuaagu     3840

uguguggaag auuuugcaaa ucuauacgua gauguagaga aaaacuugag uaaaauacgc     3900

gcugucgcag acucgaugga uaauuucagc auagaagaga uaaguaauuc guuuauaaau     3960

uuacucacac uaaccgcauu aggagcccca gcagauuuua auuuccuugg ugagaaaauu     4020

ccacgaaaaa gauacacauc cacaaaagaa ugucuuaaug cuacauugau acaccaaucg     4080

aucaccggac uauacgaaac uagaaucgau cuaucaaaaa uaggugaaga auga           4134


<210>  5506
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5506
augaaaaaac ccuacucgau cgggcuagac auaggaacaa auucagucgg augggcgguc       60

auaaccgaug auuacaaagu accauccaaa aagaugaaag uccuagguaa cacagacaag      120

cacuuuauaa aaaaaaaucu gauaggugca cuauuguucg augaaggaac cacagcggaa      180

gacagaagau uaaaaaggac aucucgacgc cgauacacga gaagaaaaaa cagauuaaga      240

uaucuccaag aaauuuucuc ugaagaaaua ucaaaacuag acagcucauu cuuccaccgu      300

cuugacgaua gcuuucucgu cccagaagau aaaaggggua gcaaauaccc aauauucgca      360

acacuugaag aagaaaaaga auaccacaaa aaguucccaa caaucuacca ucuacgaaaa      420

caccuagcag auucaaaaga gaaaaccgac cucagacuaa ucuaccucgc ucuugcacau      480

augauaaaau acaggggaca uuucuuauau gaagaaucgu ucgauauaaa aaauaacgac      540

auucaaaaaa uauucaacga auuuauauca auuuacgaua acacauucga aggcucaagu      600

cucuccggac aaaaugcuca agucgaggcg aucuuuacag acaaaauauc uaaaucaacu      660

aaacgcgaac gcguauuaaa acuuuucagc gaugaaaaaa gcacuggccu auucuccgaa      720

uuucucaaac uaauaguagg uaaucaagca gauuucaaaa aacauuuuga ccuagaagaa      780

aaagcacccu uacaauucuc uaaagauacu uacgacgaag acuuagaaaa ccuccuagga      840

cagaucggug acgguuuugc agaucuauuc cucguggcua agaaacuaua cgacgcaauc      900

cuauuaagug gcauuuuaac cguaacagac ccauccacaa aagcuccucu aucagcaagu      960

augauagaac gauacgaaaa ccaccaagaa gaucuugcug cucuaaaaca auucaucaaa     1020

aauaauuuau cagaaaaaua ugcggaaguu uucagcgauc aaaguaaaga ugguuacgcu     1080

ggcuuuauug acgguaaaac gacacaagaa gcguuuuaca aauauauaaa aaaccuacua     1140

uccaagcuag aaggagccga uuacuuucuc aauaaaauug aacgagaaga uuuucuaaga     1200

aaacaacgaa cauuugauaa cggauccauc ccacaccaaa uacacuuaca agaaaugaau     1260

gcaauucuca gacgacaagg cgaacacuac uuauuccuaa aagaaaaucg agaaaaaauu     1320

gaaaaaauuc uugcauuuag aauacccuau uauguaggac cuuuagcaag agguaauaga     1380

gauuucgccu ggcuuacaag aaacucagac caggcuauaa gaccuuggaa cuucgaagag     1440

guaguagaua aggcaucauc agcagaagac uucauaaaua aaaugacuaa cuacgaccuc     1500

uaucuuccgg aagagaaagu gcugccaaaa cauucuuuau uauacgaaac cuucgcugua     1560

uauaacgaac uaacaaaggu uaaauuuauc gccgaagguc uacgagacua ucaauuucuu     1620

gacuccgguc aaaaaaaaca aauagucaac cagcuauuca aagacaaacg uaaaguaaca     1680

gaaaaggaca ucauacauua ucugcauaac guggacgggu acgacgguau ugaacuuaaa     1740

gguauagaaa aacaauucaa cgcaucucua agcacuuauc augauuuauu aaaaauuaua     1800

aaagacaaag aauucaugga caauccaaag aacggagaaa uacuugaaaa caucauccac     1860

acgcuaacaa uuuuugagga ucgugaaaug auaaaacaga ggcuagcaca auaugauacc     1920

cuauucgaug aaaaaguaau aaaagcccua acaagaagac auuauacagg guggggaaaa     1980

cuaagugcaa aauuaaucaa uggaauuaga gacaaacaga cuggaaaaac uauacucgaa     2040

uauuuaaucg augaugguga uuguaaccga aauuuuaugc aacugauaaa ugaugacggc     2100

cuaucauuua aagaaauuau acaaaaagca caagucguag guaagaccga ugacgucaaa     2160

caaguagugc aagaaauacc ugguucaccc gccauuaaaa aagggauccu acagagcaua     2220

aaaaucguag augagcuagu uaaagugaug ggucacaauc cagagucaau cgucauugag     2280

auggcuagag aaaaucaaac gacagcaaaa ggaaaaaaaa acucacaaca acguuauaaa     2340

cguauagaag acgcucuaaa aaaccuugca ccagggcucg acuccaauau acuaaaagag     2400

aauccaacag acaacauaca acuacaaaac gaccgucucu uccucuacua ccuccaaaac     2460

ggcaaagaca uguacacagg uaaagcuauu gauaucaacc aacuuucgaa cuaugacaua     2520

gaccacauua uaccacaagc uuuuauaaaa gacgauucgc uagacaacag gguauuaaca     2580

uccucaaaag auaaucgugg aaaaucagac aacgucccau caauagaagu gguacaaaaa     2640

cgcaaagccu ucuggcaaca acuuuuagau uccaaauuaa uaucagaaag aaaauucaac     2700

aacuuaacaa aagcaaagag ggggggacua gaugagagag acaaaguagg cuuuauaaaa     2760

cgacaguuag uagagacgag acaaauaacc aaacauguug cccaauuacu cgauacaaga     2820

uuuaacacag aagucaacga agaaaaccaa aaaauaagga ccguaaaaau aaucacacua     2880

aaaucuaacu uaguuucuaa cuucagaaaa gaauucggau uauacaaagu uagagaaauc     2940

aaugacuauc accacgcuca cgacgccuac uuaaacgcag ucguagcaaa agcaauacuu     3000

aaaaaauauc caaagcucga accagaauuc guauacggcg acuaccaaaa auacgaucua     3060

aaacgauaua uauuaaaauc caaagcauca aacacaaucg auaaggcaac cgaaaaauac     3120

uucuucuacu ccaaucuacu uaacuuuuuc aaagaaaaag uuagauacgc ugacggaacg     3180

auaaagaaaa gagaaaacau agaauauagc aaugauacag gagaaauugc cuggaacaaa     3240

gagaaagacu ucgccacaau aaaaaaggua cucaguuuac cccaaguaaa uaucgucaaa     3300

aaaacagaag agcaaacagu aggacaaaac ggcggcuuau uugacaauaa cauugucagu     3360

aagaaaaaag uaguugaugc gucuaaacua auacccauca aauccucauu aagcccagaa     3420

aaauauggcg gcuaugcaag accuacuauc gcguacucag uauuagucau cgcagacauc     3480

gaaaagggga agggaaaagc aaaaaaauua aaaagaauca aggagauagu aggaauaaca     3540

auucaggaca aaaaaaaauu ugaaucaaau cccguuacau accucgagga augcgguuau     3600

aagaauauua acucaaaucu aauaauuaaa cuacccaaau acucauuauu ugaauuuaac     3660

gacggacaga gacgacuacu agccucuuca auugaauuac agaaaggaaa ugaacuuauu     3720

uugccauacc accucacagc uuuacucuau cacgcacaaa gaauuaauaa aaucucugaa     3780

ccaauacaca aacaauacgu cgaggcacac caaaacgagu ucaaagaacu acugaccaca     3840

aucaucagcc uaucgaaaaa auacauucag aaacccaaug uugaacuucu cuuacaacaa     3900

gcauucgacc aggcagacaa agauauauac caauuauccg aauccuucau uucacuuuua     3960

aaauuaacau cauuuggugc accaggagca uucaaauuuu uaggaguaga aauuagucaa     4020

uccucugucc gcuacaaacc aaacucgcaa uuuuuagaua ccacacuuau acaccaaucg     4080

auuacaggac ucuaugaaac gagaaucgac cuaaguaaac ucggagaaga cuga           4134


<210>  5507
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5507
augaauaaca aacccuacuc cauaggacuc gauauuggaa caaacagugu ggguuggagc       60

gucaucacag augacuacaa aguaccaucu aaaaaaauga aaguacuugg gaacacugac      120

aaaagauuca uaaaaaaaaa ucucauagga gcccugcuau uugaugaagg uacaacggca      180

gaggcaagga gacuaaaacg cacagcacga agacgcuaca cccgcagaaa aaacagacug      240

cgauacuuac aagaaauuuu ugcagaggaa augucaaaag uagacagcuc cuuuuuucau      300

cguuuagaug auaguuuucu aauuccggaa gauaaaaggg gcucgaaaua uccuauauuc      360

gccacguugg uugaagaaaa agaauaccac aaacaauuuc ccacgauaua ccacuuaaga      420

aaacagcuug cagacucaaa agaaaaagca gaucugcgau uaaucuaccu aguauuagcu      480

cacaugauua aauaucgagg acacuuccua uacgaagaag cauuugauau aaaaaauaau      540

gauauccaaa aaaucuucaa ugaauucauc ucaauauacg acaacacguu ugaagguagu      600

ucucugagcg gacaaaaugc acaaguagaa accauauuua cggauaaaau auccaaauca      660

gcgaaaaggg aacgcgugcu aaaacucuuu ccagaugaaa aaucaaccgg auuauuuucg      720

gaauucuuaa aacuaauugu aggcaaccaa gcugauuuua aaaaacauuu cgauuuagaa      780

gaaaaagcuc cacuacaauu uucaaaagau acauacgaug aagauuuaga aaauuuacuc      840

gaacaaauag gugaugacuu ugccgacuua uucguagccg ccaaaaaacu auaugacgca      900

auucuauugu caggcauccu cacagucaca gacccgucua ccaaagcacc auuaucagcc      960

ucaaugauag aacgcuacga aaaucaucaa aaagauuuag cuguacuaaa acaguucauc     1020

aaaacuaacc uacccgaaaa auaugucgaa guauucucag accaaucaaa agauggguac     1080

gcaggauaca ucgacggaaa aacuacacaa gaagcguucu acaaauacau aaaaaaccuc     1140

uuaagcaaau ucgacggagc agacuacuuu uuagacaaaa ucgaaagaga aguauuucuc     1200

cguaaacagc guacuuucga uaauggaagu auuccacacc aaauccauuu acaagaaaug     1260

aauacuauuc uaagacgcca aggagaacau uacccuuuuu uaaaagaaaa uaaagaaaaa     1320

auugaaaaaa uccuuacauu ccggauccca uaccauguag gaccacuagc ucgugguaau     1380

cgagacuucg caugguuaac aagaaacucc gaucaagcua uuagaccgug gaacuuugaa     1440

gaaauagucg acaaagccag uagugcagaa gacuuuauca acaaaaugac aaauuaugac     1500

cuauaucugc cggaagaaaa aguacuucca aaacauagcc uacucuacga aaccuuugca     1560

guauacaacg aguuaacaaa aguuaaguuu auagccgaag gaaugcggga uuaccaauuu     1620

cuggacucag gccaaaaaaa aaaaauaaua aaucaauuau uuaagaaaaa acgcaaagua     1680

acugaagaug auauaauaga cugcuuacaa aaaauugaua auuaugacgg aaucgaauua     1740

aaaggaaucg aaaaacaauu uaacgcuuca cuuucuaccu aucacgacau ccugaaaaua     1800

auacgagaua aggaauuuau ggaugaccca aaaaacggug acauacuaga aaauguuauc     1860

cauacacuca caaucuuuga agaccgagaa augauuaaac aacgauuagc acaauauaac     1920

agcauauuug acgaaaaagu aauuaaagca uuaucacgca aacauuacac aggaugggga     1980

aaacuuucag ccaaauuaau ugauggcauu agagauaaac agacagguaa aacuauccuc     2040

gauuacuuga uggaugaugg uaagucaaac agaaacuuca ugcaacuaau aaaagaugau     2100

gaauuauccu ucacagaaau aauccagaaa gcucaagucg ucggggacac agauaaccuc     2160

agacaagucg uacaaaaccu uccuggcagu ccagccauaa aaaaaggaau ccuacaaucg     2220

auaaaaauag ucgaugaacu cguuaaaguc augggacacg caccugaaag cauaguuauu     2280

gaaauggcaa gagaaaacca aacaacggcc aggggcaaaa agaauucuca acaaagauac     2340

aaaagaauag aagauucccu uaaaaacuug gcaaguggau uagacucgaa cauucuuaaa     2400

gaaaacccua cggacaauau ucagcuucaa aacgaccguu uauuccuaua cuaccuucaa     2460

aacggaaaag acauguacac aggagaagcc uuaaauauaa accaacuaag cagcuaugau     2520

auagaccaua ucauaccaca agccuucaua aaggacgauu cacuagacaa caggguccua     2580

accaguucaa aagauaacag aggaaaauca gacaacguac cauccuuaga aguaguagaa     2640

aaaaugaaag cauuuuggca acaauuacug gauagcaaau uaaucucuga aagaaaguau     2700

aauaacuuga ccaaagcaga aagagaaaga ggaggacuaa acgaauuaga uaaaguagga     2760

uucauaaaac gacaauuagu agaaacacga caaauaacaa aacacgucgc acaaaucuua     2820

gaugcacgcu ucaauaaaga gguaaccgaa aaaaacaaaa aaaaccguaa cgucaaaauu     2880

auaacauuaa aaagcaacau gguaucuaac uuccggaaag aauucggacu auacaaagua     2940

agggaaauca augauuauca ccacgcgcac gaugcguacu uaaacgcggu uguugcaaaa     3000

gcaauucuaa aaaaauaccc aaaacuagag ccagaauuug uguaugggga uuaucaaaaa     3060

uacgaucuaa aacgguauau aaguagaagu aaagauccaa aagaaguaga gaaagcuaca     3120

gaaaaauauu uuuucuauuc aaacuuauua aauuucuuca aagaagaagu gcauuaugca     3180

gaugguacaa uaguaaaacg agaaaacaua gaauacagca aagauacugg ugaaaucgca     3240

uggaacaaag agaaagauuu ugcaaccaua aagaaaguuc uaucgcuccc ccaagucaac     3300

aucgugaaaa aaacagaaga acaaacagua ggacaaaacg gaggucuauu ugacaacaau     3360

aucguaagua agaaaaaagu uguagacgcc ucaaaacuaa uaccuauaaa aagcggauua     3420

ucucccgaaa aauacggcgg auaugcuaga ccaacaauag cauacagcgu acuaguaaca     3480

uacaaacaaa auaauaaaau aaaaaauacc augguaggca uaccaguguu aacaaaauua     3540

gaauuugaac gagauccuaa uuuguauuug agaaauuugg gaauuaacaa cgucagccac     3600

guuauaaaac uagucaaaua cacaguacuc gaauucaaac acaaauaugg aacauacaga     3660

cgauucauag uaucaaauca agaacucaaa cgagcaaauc aaauauuugu aaccacuaag     3720

caaaugaaac uuauaucuca auacaccaca uuagauaaaa augaacauga cucauuacug     3780

aagaauaacc aacaacuaau acaaagcauu uggaaccaau uccucgaauu cauguuagcu     3840

acagaucuug uuaauaaaaa acaguacgac gaauugauac uaacaaacaa ucaaacaaua     3900

gaagaaugcc guaacauuau gaacagagug aucacuacac uaagcuucac aaauauaaac     3960

gccggacaaa agaagauaau cuuugaugac acacucuccc uaccgauaaa acgauggcaa     4020

uucacugacu ccgacaaaau ggucaagaaa auaauagaau caacuuuaau acaccaaucc     4080

aucacgggcc uuuacgaaac ccggauagac cuaagcaaac ugggagaaga uuga           4134


<210>  5508
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5508
auggaaagua accacgaugg agaacaagua aaauauacau uaggucuaga cauagggaca       60

agcucuaucg gcuacgcggc gaucgacaaa caucaaaaac caauaagagc aaaaggaaaa      120

cauguaauag guguccgauu auuccaagaa ggacaaaccg cagcugacag acgagcuuuu      180

cgaacaacca gaagaagacu aaaaagaaga aaauggagac ucaacuugcu uaaucgacuu      240

uucgauccau aucuaucaga aguggaucca aacuuuuugc cccgacuaag gcaaaguaau      300

cuaaguccaa aagacgacaa uaaacauuuc aaugggucaa cacuuuuucc ugauaaaacc      360

gaugcugcau ucuauagaca auaucccaca auguaccacc uaagauacgc auuaaugacc      420

gagaaaagaa aauuugacau aagagaagua uacuuagcaa uucaccauau uguaaaauac      480

agagguaauu uucucaauag cgcgacugua gacuccuuca aaacaaguaa cauagacuuc      540

acuucccaau uugacagauu aaacgaacua uauagacaag ugauacuuga cgaaccuuuc      600

caaauaaaca uggaucaagu agacgaaaug acaaauaaau ugcucgacaa ugacgcccuc      660

aaacuugaua cccaaaaaca aguagcgaaa uuacucccgg uaauauacaa ugacaaacca      720

auagauaaac aauauacuag aauugcuaca gaguucagua aagccaucuu aggguauaaa      780

acaaaauuag auguaauucu uaaccucgac acacaaaaug ccaaagacug gguaauucga      840

cuagaugacg aagacauaga cgacaaauua ccagccuuag uagaaaaccu ugacgaauca      900

cgccaggaaa uaguaacaau aauacgcgau cuauaugccc aaaucacuuu aaaugcuaua      960

guuccaaaag gaaaaagucu cucagaaagc augguugaua aauacaacga ucauaaagac     1020

caccuggacc uauuaauggg ccuaauccac gaacuagggg acgacucggc aaaaggaaua     1080

aaacuacgag aagcauauac acaauaugua gguaaaucag acgacaaaac acugaaucaa     1140

gaugauuuuu augcagcuau uaaaaagaau cuagaugaag acucaaagcg aucuccaaaa     1200

auacaacgac ucauagaaca agcauccuuu augccaaaac aaagaaccuc ugcaaacgga     1260

guaauaccac accaacucca ccaacuagaa cucgaucgua uaauagaaaa ccaaggacgg     1320

uauuaccccu uucucaagaa accaaauccc aauacucaua aaccaaacgg aggaaaauac     1380

aaacuagaug aacucguggc auuucgaaua cccuauuacg ucgguccacu aauuacaaaa     1440

cgugaccaag agaaaacauc cggagcagac uuugcaugga ugauaaggaa accaggucaa     1500

gaaggagaaa uaacuccgug gaauuucgac caaaagguug accgaauggc cucugcaaau     1560

cguuucauca gaagaaugac aacaaaagau ucauaucugg ucgcagaaga cguacuccca     1620

gacgccuccu uaauauacga aaaauuuaaa gcacuuaaug aauuaaacau gcucaaagua     1680

aacgauucuc gauugagucc aucccaaaaa caagaucucu uuaacaaucu auuuaagaaa     1740

cagaaaacca uaagaacaaa gaaauuacaa acauauauaa gaacuaauug ggaauugcca     1800

agugucauga uaucaggacu agccgaccca gacaaauuua auuccagucu aucaacguac     1860

auagacuucc gugacauauu uggagaaaaa gucgacgauc caaaucgaca agcagacuac     1920

gaagaaauca uugaaugguc cacuguuuuu gaagacagaa aaauauacca agcaaaacuu     1980

aaccaacuag acuggcuaac agaaccucaa agaaaagccu uacuaacaaa aagauacaca     2040

ggcugggggc gauuaucaaa gaaauuacuu acaggucuca aagaucaaaa uggugacagc     2100

aucuuagauc aauuauggaa aacaaaugau aacuuuaugc aaauccaauc gcgagacgag     2160

uucgcaaaac aaauacauga ugaaaacgca aaacaauucc acgaagccaa caauguagau     2220

gauauacuag augaugccuu uacaucucca caaaacaaga aagcaaucag gcaagucgac     2280

aaagugguac aagacguagu aaaagccguu ggauacgccc cagacaaaau cgcuauagag     2340

uucacaagau cauuggaaaa uaauccacaa agaacuguau cgagacaacg acaacuacaa     2400

gccgcauaca aaaacauacc ucgugaaauc augaaaucag guuuaauggc ugaacuagga     2460

uccguagcag auucaaagaa aguacuaucc gacaaacuau aucuauauuu uacucaaaug     2520

ggcaaagaca uguaugaaga uaaaaccaua aacaucgaca accugccgaa uuaugauauc     2580

gaccacauac uaccacaagc auuuauaaaa gacgauagca uagauaauag aguucuuauc     2640

uccaaagaag aaaaugcgag aaaaucaaac cgaguaccau uaaaauucuu uggugcaaaa     2700

agaaugggau uuuggaaaga uuuacuaaac aauggacuca uuucgaaccg caaauuccaa     2760

aaccuaacaa ccgacccaga gaaaauauca aaauauagcg cgaaaggauu uauucacaga     2820

caauuagucg aaacuuccca aguaauaaga uugguggcaa acauacuagg aaaugaauac     2880

ucacaagccg gcacaacaau aauagaagua agcgcaaaaa ugaaccgaca acuaagagaa     2940

gacuuucauu uaaucaaaaa uagaaauguu aaugacuacc aucaugcaau ggaugcuuac     3000

uuaacagcuu acgucgguga uuauuuauau cuaagauauc caaaacuaag accauacuuc     3060

guauacggug auuucaagaa agcagaagac caagaccuua auauaagaaa uuuuaacuuc     3120

uuacaugacc uuaaagauaa ugaaagucuc gauaaaauag uugauucuga aacuggugaa     3180

auaguuuggg aaaaacacgg cucaguuaaa caacucaaga aagucuauca cuauaaauuc     3240

augcugguua gucaagaagu auauacaaga caagaugcuu uauuuaauca gacaaucuau     3300

cccgcaagug augcagauaa aagaaaauua aucccuauua aaaacucaaa accgguggac     3360

guguacggag gguacuccgg aaauaucgau gcuuauaugg ccauuguacg uguccacgga     3420

aagaaagaag auaacuacaa aguggucgga aucccuauga gagcuguaac gaaacuaaag     3480

aaagcagaaa aagaagguca ugauaguuau cugaaugccg uacaugaagu acuaaaacca     3540

caauuuacca aaaagaagaa aaaccguaaa acaggcgaaa uuacagaaac aguagaugag     3600

uucgauguac uauuagggaa agucuauuac agacaacuca ucguagaugg aaauaagaaa     3660

uuuaugcuug guucaucauc uuaucaauau aacgcaaaac agcuaguccu uagugauaaa     3720

gcgaugcaag uacuaagcaa aaacggcaaa guuaaaucac aagacgaaaa ccaaaauuua     3780

auagacguau augacgaaau auuagaaaaa guagaccaau acuuugaacu auaugaucaa     3840

cgccacuauc gagaaucacu acacaaagga aggggaaucu uuguaaauuu gccacuauca     3900

aaccaauaug auggaagcaa acuuacauca uaugguaaau acgaaacgau ugacgcuauc     3960

cuaaacggac uacacgcaaa ugcaacaaug agcaacuuaa gauacuuagg gauaucaucu     4020

ccauuuggaa aaaugcaagc cucuaacgga uuaacaaucg acccagauag uauaauuugu     4080

uaucaauccc cuacaggacu guucgaacga caaauuaaau ugucggaccu cuga           4134


<210>  5509
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5509
augaauaaac cauacucaau aggacuugau aucgguacca auucaguggg augguccauc       60

auaacagacg auuacaaagu acccgccaaa aaaaugagag uauuaggaaa uacagacaaa      120

gaguacauca aaaaaaauuu aaucggugca cuacuauucg acggagggaa cacagccgcc      180

gaccgaagau uaaaaagaac agcaagacga cgguacacga gacgaagaaa ccguauacua      240

uaccuccaag aaauuuucgc cgaagaaaug ucaaagguag acgauuccuu cuuccaucga      300

cuagaagaca gcuuuuuagu cgaagaagau aaacggggau caaaauaucc aaucuucgca      360

acaaugcaag aagagaaaua cuaccaugaa aaauucccaa caaucuacca ccugcgaaaa      420

gaacuagcag acaaaaaaga aaaagcagac uuaagauuag uauaccuugc acuagcccac      480

aucaucaaau ucagagguca cuuccuuaua gaagacgacc gauuugacgu gagaaauaca      540

gacauacaaa aacaauacca ggcuuuuuua gaaauauucg auacaaccuu cgaaaauaau      600

caccuauuau cacagaaugu cgacguagaa gcuauacuca cugauaaaau aucgaaaagc      660

gcaaaaaaag auagaauucu agcacaguau cccaaccaaa aaucaacagg aauauuugca      720

gaauucuuaa aacucauagu aggaaaucaa gcagauuuca aaaaacacuu caaccuagaa      780

gauaaaacac cacuacaauu ugcuaaagac uccuacgacg aagacuuaga gaaucugcua      840

ggacaaaucg gcgaugaauu cgcugacuua uucucagugg caaaaaaacu auacgauagu      900

guacuucuga gugguauauu aacagucacu gaucuaucua caaaagcacc cuuaagcgcc      960

uccaugauac aaagauauga cgagcaccac gaagaucuaa aacacuugaa acaauucgua     1020

aaagccagcc ucccagaaaa cuacagagaa guauuugcag acaguaguaa agauggauau     1080

gcugguuaca uagaaggaaa aacuaaccaa gaagcauuuu auaaauaccu cuugaaauua     1140

uuaacaaagc aagagggcuc ugaguacuuc cuagaaaaaa uaaaaaacga agacuuccug     1200

cgaaagcaaa gaaccuuuga caacggaucu auuccacacc agguacaccu caccgaauug     1260

cgcgcaauca uaagaagaca aagcgaauau uacccuuucc ugaaagaaaa ccaagaucgg     1320

auugaaaaaa uauugacauu ccgcauacca uacuacguag gaccacuugc ccgcgaaaaa     1380

agcgacuucg cauggaugac acguaaaacu gacgacucaa uaagaccaug gaauuucgag     1440

gaccucguag auaaagaaaa aagugccgaa gcuuucauuc acagaaugac aaacaaugau     1500

cucuaucuac cagaagaaaa aguacugcca aaacacucau uaauuuacga aaaauucaca     1560

guuuacaacg aacuaacuaa agucagauuu uuagcagaag gauuuaaaga uuuccaauuc     1620

uuaaaccgca aacaaaaaga aacaauuuuu aacucgcuau ucaaggaaaa gagaaaaguu     1680

acugaaaaag auauaaucuc auuccucaac aaaguagaug guuacgaagg aauagcaaua     1740

aaagggauug agaagcaauu uaacgcgucc cucucuacgu aucaugauuu gaaaaaaaua     1800

cuagguaaag acuuuuuaga caacaccgau aaugaauuaa uacuagagga uauaguacaa     1860

acacucacuu uauuugaaga cagagaaaug aucaaaaagu gccuugacau uuacaaagau     1920

uucuucacag aaagccaacu aaaaaagcuc uauagaagac auuacacagg auggggccgu     1980

cugagugcua aauuaauaaa ugguauuaga aacaaagaaa aucagaagac gauccuagau     2040

uacuuaauag acgaugguag cgccaauaga aauuucaugc aacuaauaaa ugacgacgac     2100

cuaaguuuca aaccaauaau ugacaaagca agaacaggau cacauucaga uaauuuaaaa     2160

gaagucgucg gugaacuagc aggaucacca gcaauaaaaa aaggaauacu acaaucccuu     2220

aagauugucg acgaauuagu aaaggugaug ggauacgaac cugaacaaau aguaguagaa     2280

auggccaggg aaaaccaaac uacagcaaaa ggccuaucua ggucccgaca acgauugaca     2340

acacuuagag aaucuuuagc aaaucuaaag ucuaacaucu uagaagaaaa aaaaccaaaa     2400

uacguaaaag aucaaguuga aaaucaucac uuaucagaug aucgacucuu ccuuuacuac     2460

cuucaaaaug gucgagacau guacacaaaa aaagcacucg acaucgauaa ucuaucacaa     2520

uacgacauag accacauaau accacaagca uucauaaaag augauucaau agacaaccgu     2580

guacuaguca gcucagcaaa aaauagaggg aaaucagaug acguaccguc uauagagaua     2640

guuaaagcac gcaaaauguu cuggaaaaac uuauuagacg caaaauuaau gucccaacgc     2700

aaauacgaca acuuaacaaa agcagaaagg ggaggccuca caucagauga caaggcacgc     2760

uuuauacaga gacaacuugu agagacgaga caaauaacca aacauguagc aaggauacua     2820

gacgaaagau uuaacaauga aguagauaac gguaaaaaaa uauguaaagu aaaaauagua     2880

acacuaaaau ccaauuuggu aaguaauuuc agaaaagaau uugguuuuua uaaaauuaga     2940

gagguaaacg acuaucauca ugcacaugau gcauaucuaa acgcaguagu ugcaaaagcc     3000

auacuaacca aauacccaca acuagaacca gaguuugugu auggaaugua ccgacaaaaa     3060

aaacuaucaa aaauaguaca ugaagacaaa gaagagaaau auucagaggc gacaagaaaa     3120

auguucuucu auuccaaccu gaugaauaug uuuaaaagag ugguccgauu agcagaugga     3180

ucaauaguug uacggccagu uauagaaaca ggacgauaca ugcgaaaaac ugccugggac     3240

aaaaaaaaac auuucgcgac agucagaaaa guacuauccu auccacaaaa caacauagua     3300

aagaaaacag aaauccaaac aggaggcuuu ucaaaagaau ccauacuagc acacggaaau     3360

ucagacaaac ucaucccucg aaaaacaaaa gacauauacc uagacccuaa aaaguaugga     3420

ggauucgauu cgccaauagu cgcauacagu guacucguug uugcagacau aaaaaaagga     3480

aaggcacaaa aauuaaaaac agucacggag cuccuaggga uuacaauaau ggaaagaagu     3540

agauucgaaa aaaacccauc agccuuucua gagaguaagg gcuaucucaa cauaagggau     3600

gauaaauuaa ugauauuacc gaaauacagu uuauuugagc ucgaaaacgg gcgacgaagg     3660

uuacuagcaa gcgcaggaga acugcaaaaa gguaaugaac uagcacuacc uacacaguuc     3720

augaaauucu uauaucuagc cagccgauac aacgaaagua aaggaaaacc cgaagaaauc     3780

gaaaaaaaac aagaauuugu gaaccaacac guauccuauu ucgacgauau acuacaacua     3840

aucaacgauu uuucaaaacg aguaauucua gccgacgcaa acuuagagaa aaucaauaaa     3900

uuauaccagg acaacaaaga gaacauuccu guagacgaau uggccaacaa caucauaaau     3960

uuauucacgu uuacuucauu aggagcgccg gcugcauuca aauuuuucga caagauaguu     4020

gauagaaaac gauauacuuc aacaaaggaa guauuaaacu cuaccuuaau ccaucagagu     4080

auaacaggau uguaugaaac ccguauugac uuaggcaaau uaggggagga uuga           4134


<210>  5510
<211>  4134
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5510
auggaaaaga cauacucaau aggacuagau auaggcacca auucaguagg gugggcaguc       60

auaacagacg auuauaaagu gccagccaag aaaaugaaag uucuaggaaa uacagacaag      120

aaauacauca agaaaaauuu acucggagca cuauuauuug auucaggcga aaccgcagaa      180

gcaacuagau uaaaaagagc agcucgacga agauacacca gacggaaaaa cagacucaga      240

uaucugcaag aaauauuugc aaaagaaaug gcaaaaguag acgaaucuuu cuuccaaaga      300

uuagaagaau cuuucuuaac agaugaugau aaaacauuug acucccaucc cauauucggc      360

aacaaagcag aagaagauac guaccaccaa gaguucccua ccauauacca ccuacgaaaa      420

cacuuagcgg auucacccga aaaagucgac cuuagguuag uauauuuagc acuugcccau      480

augauaaaau ucagaggaca uuuccuaaua gaaggacaac ucaacgcaga aaauaccgac      540

guucagaaaa uuuucgcaga cuucgucgga guauacgacc gaacuuucga cgauucacac      600

uuauccgaaa uaacaguuga ugccgccagc auucuuacag aaaagauuuc caaaagcagg      660

agguuagaaa auuuaauuaa acaauaccca accgaaaaga aaaacacacu uuucggcaau      720

cuuguagcau uagcauuagg cuuacaacca aauuucaaaa caaacuucaa acuaucagaa      780

gaugcaaaau uacaauuuuc uaaagacaca uacgaugaag accuggaaga auuauugggc      840

aaaauaggag augauuaugc agauuuauuu acagcagcca aaaaucuaua cgaugcuauc      900

cuacucagug ggauccuaac cguugaugau aacuccacua aagcaccucu gagugcauca      960

augauaaaac guuacgaaga acaucacgaa gaucuagaaa aacucaaaac auucauuaaa     1020

gucaauaacu uugacaaaua ccacgaaaua uucaaagaca aaagcaaaaa uggcuacgca     1080

ggguacauag aaaacggagu aaaacaagac auauuuuaca aacaucuaaa aagcaucauc     1140

ucagagaaaa auggaggaca auacuuuuug gacaaaauag aaagagaaga uuucuuacga     1200

aaacaacgaa ccuuugauaa cggcuccauu cccuaucaga uacacuuaca agagaugcgu     1260

acuauacuaa gacgccaagg agaauauuau ccuuucuuaa aagaaaacca agcgaaaaua     1320

gagaaaauac uuacuuuccg cauuccauac uacguaggac cacuagcaag gaaaaauuca     1380

agguucgcuu gggccaaaua ucacagcgac gaaccaauaa cacccuggaa uuucgaugaa     1440

guaguagaua aagaaaaauc agccgaaaag uuuauaacaa gaaugacguu aaaugauuua     1500

uaucuaccag aagaaaaagu auugccaaaa cacucuuacg ucuacgaaac auucgcagua     1560

uacaaugagc uaacaaagau caaauauguc aacgaacaag gaaaaucauu cuucuuugac     1620

gccaacauga aacaagagau auuugaucau guauuuaagg aaaacagaaa aguuacuaaa     1680

gcaaaauuac uaucauacuu aaacaaugag uuugaagaau uucgcauuaa cgaccucauc     1740

ggacuagaua aggacaguaa aaguuucaac gcaagccuag gaacauauca cgacuugaag     1800

aaaauuuuag auaaaucauu ucuagaugac aaaacaaacg aacaaauuau ugaagacaua     1860

guauugacac ucacacuauu cgaagauagg gacaugauac augagcguuu acaaaaguac     1920

ucagauauau ucacaucuca gcaacuaaag aaauuagaac gacgacacua uacuggaugg     1980

gggcgacuuu ccuacaaacu gauaaaugga auaagaaaca aagaaaauaa uaaaacuaua     2040

uuagauuucc uaauagacga uggcgaugca aacagaaauu ucaugcaacu uauaaacgac     2100

gauucguuau ccuuuaagac aacaauacaa gaagcucagg ucguaggaga cgucgaugac     2160

auugaagcag uaguacauga uuuaccaggc ucaccagcaa ucaagaaagg aauauuacaa     2220

agcgucaaga uaguagauga guuagucaaa guuaugggac acaacccaca aaauauagua     2280

auagaaaugg caagagagaa ccagauaacc ggauauggac gaaaucgguc aaaucagagg     2340

uuaaagcgau uacaagacuc ccugaaagaa uuugguagug auauucucag caagaagaaa     2400

ccuagcuaug uagauagcaa aguugaaaac ucacaccuac aaaaugauag acuguucuua     2460

uauuacaucc aaaauggaaa agacauguac accggugaag aacuggacau cgaucacuua     2520

ucggacuaug acauagacca uauuauacca caagccuuca ucaaagacaa uucuauagau     2580

aauagaguuc uaacguccuc agccaaaaau agagguaaaa gugaugaugu acccuccauc     2640

gaaauaguaa ggaaucgcaa aucuuacugg uauaaacuau acaaauccgg acuaauauca     2700

aaacggaaau ucgauaauuu aacgaaagcu gaaagaggag gauuaacuga gacugauaaa     2760

gccggauuca uaaaacguca acucguagaa acaagacaaa ucacaaaaca uguugcgcaa     2820

auacuagaug cuagauucaa cacaaaaugc gaugaaaacg auaaagucau ccgagaugua     2880

aaagucauaa cauuaaaauc aagccuagua ucucaauucc gaaaagaguu caaguucuau     2940

aaaguacgag aaauuaacga uuaccaccau gcccacgacg ccuaccucaa cgcaguaguc     3000

ggaacagccc ugcugaagaa auaccccaag uuagcuccug aauuugugua cggagaauau     3060

aagaaauaug acgucagaaa acuugucgca aaauccagcg auaaccauuc cgaauuagga     3120

aaagcaacag caaaauacuu cuuuuauucg aaccuaauga acuucuucaa aaccgaagua     3180

aaguaugcag augguagggu auucgaaaga ccggacauag aaaccaacgc agacggcgaa     3240

gucguaugga auaaacaaag ggauuuuaau auaguacgga aaguacucuc uuacccacaa     3300

guaaauauag uaaagaaggu agaaguccaa acuggcggcu ucagcaaaga auccauccuc     3360

ccgaaaggag auucagauaa auuaauaccc cgaaaaacaa agaaacuuca augggaaaca     3420

cagaaauaug gaggauuuga cucaccuaca guagccuaca gcguacuugu aguagcagac     3480

guagaaaaag gaaaaacaag aaaauuaaaa acaguaaaag agcuaguagg aaucucaauu     3540

auggaaagaa gcucuuucga agagaaccca gucuccuucc uagaaaagaa aggcuaucac     3600

aauguccaag aagacaaacu aauaaaacuu ccaaaauauu cgcuguucga guucgaaggu     3660

ggucgucgac gcuuacuugc cucugcaaca gaauugcaaa aagggaauga aguaguauua     3720

ccccaauaca ugguuaaccu uuuauaccau ucgcaacaug uaaauaauuc acauaaacca     3780

gaacaucuaa acuacguaaa acagcacaaa gacgaauuua aagauaucuu caaccuaauc     3840

auaucuauag cgagaaucaa uauauugaaa ccuaaagugg uagauaaucu gauaaaugag     3900

uucacagaau acggacaaga agacauuuca uccuuaucag aaucauuuau caaucuucug     3960

aaauucauuu cauuuggagc uccaggcgcc uucaaauuuc uaaaauuaga uguaaaacaa     4020

agcaaucuga gauacaaauc aacaacagaa gcacuaucug caacauuaau acaccaaagc     4080

gucacaggac ucuacgaaac ccgaaucgac uuaucaaagc uaggggaaga guga           4134


<210>  5511
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5511
augaaaacuu uugcagaaga auacguaaug gauaaaucga aaccuuacgg aauuggccuc       60

gauauaggaa ccaauucugu gggauuugua gcaacggaug cugaaggaca ccuaauaaga      120

uuaaaaggua aaacaguaau uggcgcauac cucuuuaacg caggaauuuc agcagcagaa      180

cgacgaggau uuagaacgac aagaagaaga uuaaguagag uaaaauggag acuaggacuu      240

cuacgcgaaa uauuugagac acacuuccaa gaaucaaugg gagaaaacga ggacaacgac      300

uuuuucuuac gauuuaagua uucaaacauc ucgccaaaag auccucaauu cagcacugcu      360

aaaggucuau uuaaugaccg aacagauaaa gaguucuacg accaauaccc aacaaucuac      420

cauuuaagac gagcacuaau gacagaagac caucaauucg auauaagaga aaucuauauc      480

gcaaugcacc acaucguaaa auacagggga cauuuccuua aagaagguag ggcaaaagac      540

uuuaaagugg gagaucuaag acuauuagau aacuucaaaa ugaugaauga acaaaucgaa      600

gaaauuaacc cacuauggca gcuuaaacuu cccacggacg acgcaaguau aaaaucaaua      660

acagcaauau ugcucgauaa cacgcaaagc caaaaugaua ggcaaaaagc uguaacgaaa      720

guaauauugg caacuuuggu aaaagcaagc gacaaagaua uaaaugcugc aagaaaaaga      780

uuuguagggg aauuauccaa agcaaugguu gguuuaaaga ccaaauuaug gguuuuagcc      840

gauguuaguc aaaaugguga cugggaaauc aaauacgaaa auuacgcaga cuuugcagaa      900

acuauagguu ccggagaaag ugacacaaua caaucacuau uuaacgaaau aaaugaucua      960

uacggcguaa ucacacucgc agguauuaua ccaaaggaag cagaaucuuu cagcgauggg     1020

augguacgaa aauacgaaca ucauaggaaa aaccuugaac uuuuaaaagu guauugcgcc     1080

gaacaaagug acggaaaaag aggccgccag auucggcaaa cuuacgauaa auauauagac     1140

ggaguagaua gcaaacaguu uacacaagaa gauuuuuaca aagcauuaag caaauuuaca     1200

gccaaggacg aagcgacuag cgaaaaugcg aaacucauag cucaagaaau ugcaguagga     1260

acauuuaugc ccaaauuaag aacaaaagca aaugguacaa uaccacauca acuccaccaa     1320

aaagaacugg augcaaucau agaaaaccaa aagaaauacu acccauggcu aggagaagua     1380

aacccaguag aaucacaccg aagggccuua ccauacaaac uagaugaacu ugugucuuuu     1440

cggaucccau acuauguagg acccauggua acgccaacga aaggagaucc agagaaaagu     1500

aaauucgccu ggaugguaag aaaagaacca ggaacuauaa caccauggaa uuucgaccaa     1560

aaaguagaca gauccgccuc gggugaggcu uucauccaac gcaugaaaac gacagauaca     1620

uuucucauug gagaagaugu acuaccacaa caaagccuau uauaccaaaa auucgaagua     1680

cuuaaugaau uaaacaaaau uaugauuaau ggcaaaccaa ucuguagaga acagaaacag     1740

agacuguuua aacaacucuu caugcaguac aaaacaguaa caguaaagaa aguacaacaa     1800

aaucucauug ccaauggcga agaaucggaa aacguaccaa uuacaggucu aagcgauccc     1860

cuuagauuca auuccucauu uaguacauac auagacuaca aagacauucu ugggaccgca     1920

gcugucaacg auaacgcgaa acaaagcgau aucgaacaaa ucauugccug gaguacaauu     1980

uuugaagacg cagccauauu uagagaaaaa uuaaaugaua uaacguggcu aaaugacgau     2040

caaagaaaca aacuaagcca uaaaagauau cgcggauggg gacggcauuc gcgaaaauua     2100

uuagcaggau uaagagaugg ugaaggacaa acuauaauag aacggcucug gaauacaaau     2160

gauaauuuca ugcaaauaca aaacgacucc gaaauagcca gacaaauaac agaggcuaau     2220

ucaagcaaaa uggcaacagc ugaagguaca gacgaaauaa uagacgguuu uuacaccuca     2280

ccagaaaaua agaaagcccu ucgagaaguc augaaaguag ucaaagauau ucaaagagca     2340

caucaugguc aagcgcccgc cuggguuuac auagaauccc caagagaaac uccacgucca     2400

gggcaaagaa cugugagcag agaacaacaa cuaacagacu uauaugaagg agcagcaaaa     2460

gaaauaguag augacgccgu auuaaacgaa cuaaaagaua agguaaaauc aaaagaaaac     2520

uucacagaua aauuaguauu auacuuuuua cagaacggac acgauauaua ugcaaacgau     2580

ucaaucaaca uugacaaccu caacgcauau gauauagauc acguacuacc acaaucacuu     2640

auaaaagaug auucauuaga caaucgagua uuaacgaccc augaaagaaa ucugaagaaa     2700

agcaacagau ucgcuacaga acuauuugcg gaucaaagaa agaaauggga aaaauggcau     2760

cggcuaggac uaaucucauc ucgaaaauua aaacauuuaa cuaugcaacc aaauagcgua     2820

gagaaauucg cacacgguuu uauagcaaga caacuaaccg aaacgagaca aauaauucac     2880

uuaacagcua augugcucuc aaaccuuuau caagaaaaug acacgaaaau cgucaugauc     2940

aaagcuggcc uuaauuccga guuccggaga acauucgacu uuccuaaaaa uagaagugua     3000

aacgacuauc aucacgcauu cgaugcauuc cuaacagcaa aaauaggacg auacuuauua     3060

gcaagauacc caaaacuaga accuuucuuu guguacggua acuuugucaa aaacccaaaa     3120

gcgaugaaaa ggcuaaguag uuuugacuuc auugcacaac uagcugccaa aaccgacgac     3180

acgucucaca uagaucaaag auccuuaaaa caaguuccag ucguaaauga agaaacggga     3240

gaaauugucu gggacaaaga uauagagcuu gcagaauuag acaaaacgua uaauuacaaa     3300

acgauguuag ucaaaagagc acaaacagaa aacaacgcuc aaauguucaa acaaacaguc     3360

uucaaagcaa gagacaauca aaauaaaacc cuaauaccag ugaaaaaugg uuuaucgacc     3420

gauguauaug gaggacauuc gcagcaagca auaagcuauu uaugcauugu auggguggga     3480

caaaagaaga aauacagagu ucucggaaua agcaccgcac augccgggau cuuaaauaau     3540

uucgagaaaa auuacggccg uuucgaagcg aaaaagaaau uacaagaaau cguaucaaau     3600

acauuggaua augcagaucg aaaugacuuc aaaaucguag cacccaaagu acuauucgaa     3660

caagucgucg aagacgacaa uaugaaauuu ggacuuggau cugcuucaga uuacagaaac     3720

guccaacaac uauuccuuuc acguaaaaac caacuacuuc uagcuaauau gaugacagau     3780

caaauacacg accaagaccu aguucaccua uucgaugaaa uaguaggaca aaugaacgcg     3840

cacuucccca uuuuugauag aggaggauau agaagcucgc uaacucaguc aagggacaaa     3900

uuucuaaaac uaccauuuaa gaaaaaugaa gaucuaauua caaaacagga agucauucga     3960

cggauccucg acggucucca cgcgaacgca aacagaaaag aucuaaaaau cauaggaucu     4020

aaaggagacu uugguagauu aggaacuaag aaaauauauc uauccaaaga cgcaaaauua     4080

auauacacua gcccaaccug ccucuuuacu cguacaguac cucuuagcuc auuauga        4137


<210>  5512
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5512
augacaaacg aaaaauauag cauaggcuua gacauuggaa cgaguucaau cggauucgcc       60

guagugaacg acaacaacag aguaauuaga guaaaaggca aaaaugcaau agggguuaga      120

cuauucgaug agggaaaagc ggccgccgau cgaagauccu ucagaacaac acgcagaaga      180

cucucaagaa gacgcuggcg gcuaaaauug cucagagaaa uauuugacgc cuauauaaca      240

ccaguugacg aggcuuucuu cauacgauua aaagaaucua auuuaucgcc aaaagauagu      300

aagaaacaau acucagguga caucuuauuu aacgaccgaa gcgauaaaga cuuuuaugaa      360

aaauacccga cgauauauca cuuaagaaau gcguuaauga cggaacacag aaaauucgac      420

guacgcgaaa uauauuuagc aauccaucac aucaugaaau uccgcggcca uuucuuaaac      480

gcaacaccag cuaauaauuu uaaaguaggu cgacuaaacc uugaagaaaa auucgaagaa      540

uuaaacgaca uauaccaacg aguguucccc gaugaaagua uugaguuccg aacagacaac      600

uuagaacaaa uaaaagaagu acuucuagau aacaaaagau ccagagccga ucgccaaaga      660

acauuaguca gugacaucua ccaaucuucc gaagacaaag acauugagaa aagaaacaaa      720

gcaguagcaa ccgaaauacu aaaagcauca cuaggcaaca aagcaaaacu caaugugauc      780

acaaacguag aagucgacaa agaagcagcu aaagaaugga guaucacauu cgacucagaa      840

agcauugacg augaucuggc aaagaucgaa ggacaaauga cagacgaugg acaugaaaua      900

auagaaguuu uaaggucauu auauucugga auuacauuau cagcaaucgu acccgaaaau      960

cauacacuuu cacaaucgau gguagcaaaa uaugauuuac acaaagauca ucucaaacug     1020

uucaagaaac uuauaaacgg uaugacagac acaaagaaag ccaaaaacuu aagggcagcg     1080

uaugacgguu auaucgacgg cgugaaaggg aaaguucuuc cacaagaaga cuuuuauaaa     1140

caaguacaag uaaaccuaga cgacucggcu gaagcaaaug aaauucaaac auacaucgac     1200

caagauauau uuaugccaaa acaaagaacg aaagccaacg gcucaauccc gcaccaauua     1260

caacaacaag aacuugauca gauaauugaa aaccagaagg cuuacuaucc auggcucgcu     1320

gaauuaaacc cgaauccuga caagaaacga caacaauuag caaaauacaa acucgacgaa     1380

cucguaaccu uuagaguacc guauuacguc ggacccauga uaaccgcaaa agaucagaaa     1440

aaucaaagug gagcggaauu ugcauggaug auaaggaaag aaccagguaa uauaacacca     1500

uggaauuucg aucaaaaagu agaccgaaug gccacagcaa accaauuuau uaaacgaaug     1560

acaacaacag auacguacuu acuaggugaa gauguacucc cagcccaauc ucuauuauac     1620

caaaaauucg aaguucuuaa ugaauuaaac aaaauccgaa uugaccauaa accaauuuca     1680

auagaacaga aacaacaaau auucaaugau uuauuuaaac aauuuaaaaa ugugacuauu     1740

aaacaucuac aagauuacuu agugucccaa ggccaauacu cgaaaagacc cuuaaucgaa     1800

gggcuugcag acgaaaaacg auuuaacuca ucacuaagca cauauucuga ccuaugcggg     1860

auauuuggug ccaaauuagu ggaagaaaac gauagacaag aagaccuaga gaaaauaaua     1920

gaauggucaa caauuuucga agauaagaaa auauaccgag caaaacuaaa cgaccuaaca     1980

ugguugacag augaucaaaa agaaaaacug gcaacaaaac guuaucaagg augggguaga     2040

uuaagccgca aacuauuggu agggcucaaa aauagugaac auagaaauau aauggauaua     2100

uuauggauua caaaugaaaa uuucaugcag auccaagcag aaccggauuu ugcaaaacuc     2160

gucaccgacg cgaauaaagg cauguuagaa aagacagauu cucaagacgu uauaaacgac     2220

cuauauaccu caccacaaaa caagaaagcu auacggcaaa uccuacucgu aguacacgau     2280

auacaaaacg cuaugcaugg ccaagccccc gcaaaaauac acguagaauu ugcccgagga     2340

gaagaaagga aucccagaag aucaguacaa agacagcggc aaguagaagc ugcuuacgaa     2400

aaaguaucaa augaacuggu aucugcaaaa guaagacagg aguucaaaga agcaaucaau     2460

aauaaacgag acuuuaaaga uagacuacua cuuuauuuca ugcaaggagg gauagauaua     2520

uacacaggca aacaacuaaa uaucgaucaa cuaucaucau accagauaga ccauauccuu     2580

ccccaggccu uuguaaaaga cgacagcuua acaaaccgag uacuaacaaa cgaaaaucaa     2640

guaaaagcag acaguguacc aauagauaua uucggaaaga aaaugcuauc aguuugggga     2700

agaaugaaag accaaggguu aaucaguaaa ggaaaauaca gaaaccuuac caugaaccca     2760

gaaaauauaa gcgcccacac ugaaaauggu uuuauuaacc gacaauuagu ggaaacuaga     2820

caaguaauua aacuagcagu aaacauacug gcagaugaau auggagauuc aacgcaaaua     2880

auauccguga aagcagaccu cagucaucaa augcgagaag auuucgaauu guuaaagaau     2940

agagacguga acgacuacca ccacgcauuu gacgccuauu uagcagcauu uauuggcaau     3000

uaucuccuaa aacgauaucc aaaacuugaa ucguauuuug uauacggaga uuucaagaaa     3060

uuuacucaaa aagaaaccaa aaugcgaaga uucaacuuua uuuaugaucu aaaacacugc     3120

gaucaagucg uaaacaaaga aacaggugaa auauuaugga caaaagauga agacauuaaa     3180

uauauacgac aucuauucgc auacaagaaa auauuagugu cacaugaagu gagggagaaa     3240

agaggagcac ucuacaauca aacuauauac aaagcgaaag augacaaagg aucaggacaa     3300

ggaucaaaga aacuuauucg uauaaaagau gauaaagaaa caaaaauaua cggaggauac     3360

ucaggaaaaa gccuagcuua uaugacuauc guccagauua cuaagaaaaa caaaguaucu     3420

uauaggguaa uagguauacc cacacuugca cuagcaagau uaaauaaauu agaaaacgau     3480

ucaacagaaa auaaugggga auuguauaaa auaauaaaac cucaauuuac ccauuauaaa     3540

guagauaaga aaaacggaga aauaauagag acgacagacg acuuuaaaau uguaguaucg     3600

aaaguaagau uccaacaauu gauagacgau gcgggucaau ucuuuaugcu cgcgagcgac     3660

acuuauaaaa acaacgcgca acagcuagua auaaguaaua augcgcuaaa agcgauaaac     3720

aauacaaaua uaacagacug uccaagagau gaccucgagc gauuagacaa ccugagauua     3780

gauagugcau uugaugagau agucaagaaa auggacaaau acuucucagc auaugaugca     3840

aauaauuuua gagagaaaau uagaaacuca aaccuaaucu ucuaucaacu accaguagaa     3900

gaccaauggg aaaauaauaa aaucacagaa uuaggaaaaa gaaccguacu cacaagaauc     3960

uugcaaggac uacacgccaa cgcaacuaca acggauaugu caauuuucaa aauaaaaacu     4020

ccauucgguc aacuacgaca aagaucagga auaagucuau cagaaaaugc acaacuuauc     4080

uaccaaucac caacuggauu auuugaaaga agaguacaac uuaauaaaau caaauga        4137


<210>  5513
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5513
augaaaaaca aagacuauuc aauaggcuua gauauaggua cuuccucaau cggauucgcc       60

guaguugaug agaauaauca uguuaugaga gucaaaggca aaaaugccau aggcguaaga      120

cuauucgacg aaggaaaacc agcugcagac cgacgugccu uucggaccac acgucggaga      180

cugucacgaa gaagauggag acucaaauua cuaagggaaa uauuugauuc auauaucaca      240

ccaguggacg aggcuuuuuu cauaagguua aaagaaagca aucuaucccc aaaagacuca      300

aaaaaacaau auaguggaga cauacuauuu aacgauagaa gcgacaaaga cuuuuauacc      360

aaauauccaa ccauauacca ucuaagaaau gcauuaauga cggaacacca acguuucgau      420

aucagagaaa uauauuuagc uauccaccac auaguuaaau uccgaggaca cuuuuuaaac      480

gcaacaccug cgaauaauuu ucaaguagga aaacuugacu uggaccaaaa auucgaagac      540

cuuaaugaua uauaucaaag aguauuccca gacgacagua uagaauucag aaucgacaac      600

cucgaacaaa uaaaagccgu auuauuagac ucaaaaaggu cacgcagcga cagacaacgc      660

ucacuuguag acgacguaua ucaaacuuca gaagacaaag aaguagagaa acaaaacaaa      720

gcaguugcaa ccgaagucuu gaaagccaua cuaggcaaca aagcaaaacu uaacauaauu      780

gcaaauguug aaguugacaa agaggcagca aaagagugga cuauaacauu cgacuccgaa      840

ucaauagacg acgaccucgc aaaaauagac ggucaaauga cagacgacgg aagagcuaua      900

guggaaauac ucagauccuu auacagugga auuacccuuu cugcaauagu gccugaaaau      960

cacacccuua gccaagcaau gguagcaaaa uaugaucuac auaaaaagca ucucacauua     1020

cucaaaaaau uaaucaauuc gaugaccgac acuaaaaaag cuaaaaaccu ccgcgcagcc     1080

uacgacggau acguagaugg cguaaaagga aaaguacuaa cucaagauga auucuauaaa     1140

caaauacaau ccaauuugga cgaaucagua gaagcauccg aaauacaaac auauauagau     1200

caggacauuu uuaugccuaa acaaagaacc aaagcgaacg guagcauucc acaccaauua     1260

caacagcaag aacuagacca aauaauagaa aaccaaaagg aauacuaccc cugguuagcu     1320

gaacuaaauc cuaacccaga caaaaaaaga caacaauuag caaaguauaa acuugaugaa     1380

cucguuaccu ucagaguacc guacuaugua ggcccaauga uaacagcaga agcacaaaaa     1440

aaacaguccg gagcagaauu cgcauggaug auucgcaagg aaagcggcaa cauaacuccu     1500

uggaacuuug accaaaaagu agauagaaug gcuacagcaa aucaauuuau aaaaagaaug     1560

acgacuaccg acacauauuu auugggagaa gacguauuac caucgcaguc ucugcuuuac     1620

caaagauucg aaguauuaaa cgagcuuaac aaaauccgaa uagaucgaaa accaauaucc     1680

gucgagcuua aacaacaaau cuuuaaugac uuauuuaaaa aauucaaaaa uguaacaauc     1740

aaacaccuac aagacuauuu aguagcucaa ggacaauacu caaaacgacc auugauagaa     1800

gggcucgcag augaaaaaag guucaacuca ggccucucaa ccuacaacga cuucuguggg     1860

auauuugggg cuaaacuggu cgaugagaau gacagacaag ccgauuuaga aaagauaauu     1920

gaauggucua caaucuuuga agacaaaaaa auuuaccgag caaaacuaaa cgaucuagca     1980

uggcuaacug augaacaaaa agauaaacuu gcgacaaaga gguaucaagg auggggacga     2040

cuaucaagca aauuauuaau aggacuacaa aacagcgaac acagaaacau aauggaccuc     2100

cuuuggauug gaaacgaaaa cuucaugcaa auacaagcag auucugacuu ugcgaaauua     2160

auaaaagaug caaaucaaaa cgccuuacaa aacaaugacu cucaagacgu gaucaaugac     2220

uuguauacga guccgcaaaa caaaaaagcu aucaggcaaa uuuuguuagu cguacaugac     2280

auacaaaacg cuaugcaugg ucaagccccu accaaaauac auguugaauu ugcaaaaggu     2340

gaagaaagaa aucccagaag aucaguccaa agacaaaggc aaguagaggc agcuuacgaa     2400

aaagcaucaa acgaacuugu aggugcaaac guucgagaag aacuaaaaga cgcaauaacu     2460

aauaaacgag acuucaaaga ucgucuauuc cuauacuuca ugcaaggcgg gguugauaug     2520

uauagcggag aacccauaaa uauagacaau uuauccagau acgauaucga ccauauccua     2580

ccacaggcgu uuguaaaaga ugauuccuua aauaacagag uauuaaccga ugcagagaaa     2640

aauaggagaa aaagugaugc aguucccaau gaauuauuug gagccaaaau gagaguaaca     2700

ugggaacgca ugaaagaaca gggccuaauc ucaaaaggua aauaccguaa ccucacuaug     2760

aauccagaaa auauaaauaa auacaccgaa aacgguuuca uaaauaggca auuaguagaa     2820

acgcgacaag ucauaaaauu agcaguaaau auacuagccg acgaauauaa ggaaaacacc     2880

caaauaauau ccguaaaggc agauuuaucg caccaaauga gagaagauuu ugaauuauua     2940

aaaaacagag auguuaauga cuaucaucau gcauuugaug cuuauuuagc ugcguuuaug     3000

ggaaacuacu uauuaaaaag auauccaaaa cucagauccu acuucguuua uggagacuuu     3060

aaaaaauuua cucaaaagga aauaaaacua agacguuuua acuucaucua ugacuuaaaa     3120

cacgaagauc aaguaaucga caaagaaacc ggagaaauac uuuggaacaa gaacgaagac     3180

aucgcauaua uaaggcaccu auuugccuau aagaaaauau uaauuucaca ugaaguuucu     3240

gaaagaagag gcgcguugua caaccaaaca gucuacaaag cuagugauga caaggcauca     3300

ggacaaggau caaaaaaauu aauacccaua aagucagaua aaccaacaga aaucuacgga     3360

ggauacaccg gcagaacacu agcuuauaug acaauaguua agguaacaaa gggcaaaaag     3420

auauccuaua aaguaguagg agugccgaca gcaguauuag cgaaaauggc gggaguacga     3480

cagggauccc aagaagaaca agacaaauua uacggggugc uaaagcccca auuuacucac     3540

caaaaagaga acaaaaaaac aggcgaaaua accgaaacag uagacaaguu ugacaucauu     3600

gucccaaaag uuaaauauca acaguuagua aaugacaacg gacagcucuu caugauagga     3660

ucagauaccc ucccuuauaa ugcagaacaa uuggugcuuu caaaagaauc aguccaaacc     3720

aucaacaauc ccggaauaaa agacgaggga agugaugaag aaagcgaaaa caaaaaauua     3780

gacaauguau acgacgaaau aguauuaugu augaacaagu auuuuccuuu auaugacaac     3840

agagguuucc gaaacuccau cacaaaugga aaaaacaaau ucuuaaaauu accaauaaag     3900

aacauauaua aaaacgguaa acuaauaaau guaggaaaac gcgagguuau caauagaauu     3960

cuucaagguu uacacgcaaa ugcuaguguc acagaccuau caaauuuagg uauuaaaagc     4020

ccuuuuggcu ucuuacaaaa gggcgccggu cuaagccuau caggagaagc gcaacugauu     4080

caccaauccg cuaccggcuu guucgaacac cgaguacagc uaaauaaaau caaauga        4137


<210>  5514
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5514
augagcaaua aacccuauag cauuggauua gauauaggua caaacucagu aggaugggca       60

guaaucacag augacuauaa aguaccaucc aagaaaauga aaguuuuagg uaauacagau      120

aaacacuuca uuaagaaaaa cuugauaggu gcacuacuau ucgacgaagg aacgacagca      180

gaggguagaa ggcuaaaacg aacagcaaga cgcagguaca ccagaagaaa aaaccgacua      240

agauaccucc aggaaauauu cagugaagag aucucuaaag uugacaguag uuuuuuccac      300

agauuagacg acaguuuccu cguuccugag gauaaacgag gaucaaaaua uccaauuuuu      360

gcaacacucg cagaagaaaa agaauaccau aaaaacuuuc caacuauuua ccaccucaga      420

aaacaauuag cagauucaaa ggaaaaagcc gauuuaaggc uaaucuaucu ugcgcuggcu      480

cauaugauga aauacagagg ucacuucuug uaugaagaaa gcuuugauau uaaaaacaau      540

gauauacaaa aaauuuucaa cgaguucaua ucaauauacg acaauaccuu cgagggaucg      600

ucacuuagcg aacaaaaugu gcagguagaa gcaauuuuua cagauaaaau cuccaagucg      660

gcuaaacgug aaaggguuuu aaaacucuuu cccgacgaaa aauccaccgg acuuuucucu      720

gaauuucuaa aacuaaucgu agguaaccaa gcagaauuca aaaaacacuu ugacuuagag      780

gaaaaagccc cuuuacaauu uucaaaagau acauaugaug aggaccuuga aaaccuauua      840

ggccagauag gugacgguuu cgcugaucuu uuccuaguag cgaagaaacu guaugaugcc      900

auucuacucu cugguaucuu aacaguaacg gaccccucaa caaaagcgcc auuaagcgcc      960

ucuaugauug aacgauacga aaaccaucaa aaagaccuag caucacucaa acaguucauu     1020

aaaaauaauu uaccagcaga aaaaugcgua gaaguauucu cagaccaauc aaaggacgga     1080

uacgcuggau acauagaugg uaaaacuaca caagaagcau ucuauaaaua uauaaagaac     1140

cuccugucaa aauuagaagg agcguacuac uuccuagaca aaauagaaag agaagauuuc     1200

cuuaggaaac aacgcaccuu ugauaauggc agcauccccc accaaauaca cuuacaagaa     1260

augaaugcua uacuacgaag acaaggagaa cacuauccau uccuaaaaga aaacagagag     1320

aaaauagaga aaauuuuaac auuccgaaua ccauaccaug ucggaccauu agcacgcgga     1380

aacagagacu uugcuuggcu caccagaaau ucugacgaag caauuagacc uuggaauuuu     1440

gaagagauug ucgaucaggc aucauccgcu gaagacuuua uaaauaaaau gaccaauuac     1500

gaucuguacc uaccagaaga gaaaguucug ccaaaacauu cucuacuaua cgaaacuuuc     1560

gcaguauaua acgaauuaac caaaguaaaa uuuaucgcag aaggauuaac ccgcuaucaa     1620

uuccuagaua aaaaacaaaa aaaagacaua uucuauacau uuuuuaaagc agaagacaag     1680

aggaaaguaa cggaaaaaga uauaauacac uauuuacaca auguagacgg auacgacggu     1740

auagaacuua aaggaauaga aaaacaauuc aacgccagcc uaagcacaua ccacgaccuc     1800

cuaaaaauaa ucaaagacaa ggaauucaug gaugauccca agaacgaaga aauacuugaa     1860

aacauuguac acacacuaac cauauuugaa gaccgagaaa ugaucaaaca aagacuagcc     1920

caauaugacu cauuauuuga cgaaaaaguc auaaaagccc uaacccgucg gcauuauaca     1980

gguuggggaa aacuaucagc uaaacuuaua aacgggauau gugacaagca aacagguaaa     2040

acuauacuug auuauuugau agacgacgga aaaauaaaua gaaauuucau gcaacuaauc     2100

aacgacgacg gacucucguu uaaagaaaua auacagaaag cgcaaguagu aggaaaaacg     2160

uacgacguaa aacaagucgu ucaggaacuu ccaggaucac cagcaaucaa aaaaggaauc     2220

cuacagucag uaaaauuagu ugaugaacuu guuaaaguaa ugggacacgc accggaauca     2280

auaguaauag aaauggcaag ggaaaaucaa acaacagcca aagguaaaaa gaauucacaa     2340

caaagauaca agagaauaga agaugccauu aaaaaucuug cacccggucu agacagcaca     2400

aucuugaaag aacacccaac cgauaacauu caacuacaaa augaccgucu auuccuauau     2460

uauuuacaaa acggucgaga cauguauaca ggaaaaccau uagaaaucaa ccaacuauca     2520

aauuaugaua uugaccauau cauaccacag gcauuuauca aagaugacuc acuugauaau     2580

agaguucuaa caucgucaaa agaaaacaga gguaaaagcg acaauguacc aucauuagaa     2640

guuguagaaa aaaugaaggc auucuggcaa caacuuuuag acagcaaacu aauaagcgaa     2700

agaaaguuca acaaucucac aaaagcagaa agaggaggau uagacgaauu agacaaagua     2760

ggauuuauaa aaaggcaguu gguugaaaca agacaaauaa caaaacaugu ugcacaaauc     2820

cuggacgcca gauuuaacaa agaaguaacc gaaaaaaaca agaaaaacag aaauguaaaa     2880

aucauuacac ugaaaaguaa caugguuuca aauuuccgga aagaguuugg auuguacaaa     2940

guacgagaaa uaaacgacua ucaccaugcg cacgaugcgu aucuaaacgc uguaguugca     3000

aaggcaaucc uaaaaaaaua ucccaaacua gaaccugaau uuguauacgg cgacuaucaa     3060

aaauacgacc ucaaacgaua caucucaaaa ucaaaagauc cgaaagaagu ugaaaaagca     3120

acagaaaaau auuucuucua uuccaaccuu cuaaauuuuu uuaaagaaga aguucauuau     3180

gcagauggaa caauaguaaa aagagaaaau auugaguauu cuaaagauac cggagaaaua     3240

gcuuggaaca aagaaaaaga cuuugccacc aucaaaaaag ugcuaaguuu cccacaagua     3300

aacauaguaa aaaaaacaga agaacaaacg gucggucaaa acgguggacu auuugacaac     3360

aacaucguua gcaaagaaaa aguaguugac gccucuaaac uuauaccuau aaaaagcggu     3420

cuauccccag aaaaauaugg aggauacgcc cgaccaacca uagcauauuc cgucuuagua     3480

acguauaaac aaaacaacaa aaucaaaaac acaaugguag guauaccggu cuuaaccaaa     3540

cuagaguucg aaagagaucc aaaccuauac cuacgcaauc uuggaauaaa uaacguauca     3600

cauguuauaa aacucguaaa acauacagua cucgaauuua aacauaaaua uggaacuuac     3660

agacgcuuua uugucucaaa ccaagaacug aaacgagcca aucaaauuuu cgucacaaca     3720

aaacaaauga aacuaauaag ccaauauacu acccuugaua aaaaugagca cgacucuuua     3780

cuaaagaaua aucagcaauu aauucaauca aucuggaacc aauuccuuga guuuaugcuc     3840

gccaccgauu uaguuaacaa aaaacaauac gacgaacuua ucuuaacaaa uaaccaaacc     3900

auagaagaau gcagaaacau aaugaacaga gucauuacca cccuuuccuu caccaacaua     3960

aacgcaggac aaaaaaaaau uaucuucgac gauacacuua guuuacccau aaaacgaugg     4020

caauucacug acagcgauaa gaugguaaag aaaauaauug aauccacguu aauacaccag     4080

ucaauaacgg gacucuacga aacacgcaua gaccuaaaca aguuagggga agacuga        4137


<210>  5515
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5515
augaacaaac ccuauucaau agguuuagac aucggaacaa auucaguggg uuggucaauc       60

auaaccgacg auuauaaagu accagccaaa aaaaugaggg uauuaggaaa cacagacaag      120

gaauacauca aaaaaaaucu aaucggugca cucuuauuug auggaggaaa cacugcagca      180

gaccgacgac uaaaaagaac ugcucgaagg cgcuacacac gaaggcgcaa ccgaauacua      240

uauuuacaag agauuuuugc cgaggaaaug aguaaagugg augauucauu uuuccauagg      300

cuagaagaua guuucuuagu agaagaagau aaaagaggua gcaaauaucc gaucuuugcu      360

acgaugcaag aggaaaaaua uuaccacgaa aaauucccca ccauauacca ucuccgaaaa      420

gaacuagcag acaaaaaaga aaaagcugau uuaagacuua uauaccuagc auuagcacac      480

auaaucaaau ucagggguca uuucuuaaua gaagacgauc gauuugacgu gcgcaacaca      540

gauauacaaa aacaauauca agccuuccua gaaaucuuug auacgacauu cgagaauaau      600

gacuugcuuu cacaaaaugu agauguagaa gcaauccuca cggacaaaau caguaaguca      660

gcaaaaaaag auagaauccu agccagauac ccuaaccaaa aaucaacagg aaucuucgcu      720

gaauuucuaa aauuaauagu cggaaaccaa gccgauuuca aaaaacauuu uaacuuagaa      780

gacaaaaccc cauuacaauu ugcaaaagau ucuuacgaug aagaccucga aaacuuacua      840

ggacaaauag gugacgaauu cgcagacuua uuuagcgcag cuaaaaaacu auaugauucu      900

guauuauugu ccggaauccu uaccguaaua gaccuaagca cgaaggcacc ucuaucagcu      960

aguaugaucc agcgcuacga cgaacaccau gaagaccuaa aacaccuaaa acaguuugug     1020

aaagcgucau ugccugaaaa cuauagagaa guauuugccg auucaagcaa agacggauac     1080

gccggcuaca uagaaggaaa aaccucucaa gaagcauuuu auaaauauuu acuaaaauua     1140

cucacaaaac aagaagguag cgaauaucua uuagaaaaaa uaaaaaauga agacuuccuu     1200

cgaaaacaaa gaaccuucga caauggaucc auaccucauc aaguccaucu uacagaacua     1260

cgagcaauua uccguagaca aagugaauac uacccuuuuu uaaaagaaaa ccuagaccgu     1320

auugaaaaaa uccuaacauu ccgaauaccg uacuacgucg gcccccuagc ccgagaaaag     1380

ucagauuuug cuuggaugac aagaaaaaca gacgauucca uaaggccaug gaacuucgag     1440

gaccucgugg acaaagaaaa aucagccgaa gcuuuuauuc auagaaugac aaacaacgau     1500

uuauaucuac cagaagaaaa aguccuaccc aaacauuccc uaaucuauga aaaauuuacu     1560

gucuauaaug aacucacaaa gguacgcuuc cuagcagaag guuuuaaaga uuuccaauuc     1620

cucaaucgaa aacaaaaaga aacaauuuuc aauucccuuu ucaaggaaaa aagaaagguu     1680

acagaaaagg acauuauauc auuucucaau aaaguugaug gcuacgaagg uauugccaua     1740

aaagguauag aaaaacaauu caaugccucu cuaucuacau accaugaucu caaaaaaaua     1800

uuaggaaaag acuucuuaga uaacacagau aacgaacuaa uacuugaaga caucauccaa     1860

acacuaacac uauuugaaga ucgagaaaug auaaaaaaac gauuggacau auacaaagac     1920

uucuuuaccg aaucacaacu uaaaaaacuc uauagacgcc acuacacugg cugggguaga     1980

cuuucugcaa aacuaauaaa ugguauaaga aauaaagaga accagaaaac gauccuugac     2040

uauuuaauug acgacggauc ugccaaccga aauuucaugc aguuaauaaa agacgcagga     2100

uuaucauuca aaccaauaau cgacaaagcu agaacaggua gucacagcga caacuuaaag     2160

gaagucguug gugaacuugc cggcagcccg gcaauaaaaa aaggaauccu acaaucacua     2220

aaaauagucg acgaacuugu caagguaaug ggguaugaac cugaacaaau aguaguagaa     2280

auggcaagag aaaaccaaac aacugcaaag ggauugucaa gguccagaca aagacuaaca     2340

acauuacgcg aaagcuuagg aaaccuaaaa ucuaacauac uagaagagaa aaaacccaaa     2400

uacguuaaag aucaaguaga gaaucaccac cuuucagacg accggcuauu ccucuacuau     2460

uuacaaaaug gaaaagacau guauaccgac gacgaauuag acauagacaa cuuaucucaa     2520

uaugauauag accauauaau accacaagca uucauaaaag acgacaguau cgacaaucga     2580

guauuagugu caagugccaa aaacagagga aaauccgacg acguaccuag cauagaaauu     2640

gucaaagcga gaaaaauguu uuggaaaaac uuacuagacg caaaacugau gucacagagg     2700

aaauaugaua accuaacaaa agcagaacga ggugggcuaa ccagugauga caaagcacgu     2760

uucauacaac gucaacuggu cgagacaaga caaauaacua aacacguugc cagaauccuc     2820

gacgaaagau ucaauaauga auuagacucc aaaggaagaa gaauaagaaa aguaaaaauc     2880

gugacgcuua aaucgaaccu aguaaguaau uuucgaaaag aauucggauu cuacaaaauc     2940

agagaaguaa auaacuauca ucaugcacac gaugcauacc uaaacgcagu cguagcaaag     3000

gccauuuuaa cgaaauaucc ucaauuagaa cccgaauuug uauauggccu auaccgucga     3060

aaaaaacuua gcaaaauagu aagagaagau aaagaagaaa aauacuccga agcaacucga     3120

aaaauguucu uuuacucuaa cuuaaugaau auguuuaagc gagucguacg ucucgcagac     3180

ggaagcauug uaguuagacc agucauagag acugguagau acaugggaaa aacagcaugg     3240

gauaaaaaaa aacacuuugc aacgguacgc aaagucuuaa gcuaccccca aaacaacaua     3300

gucaagaaaa cggaaauuca aacaggaggu uucagcaaag aaagcaucuu agcacacggu     3360

aauucagaua aauuaauccc ccgaaaaaca aaagauauau acuuagaucc gaaaaaauac     3420

ggcggauucg auucaccaau cguugccuau aguguccuag uuguggcuga caucaaaaaa     3480

ggcaaggccc aaaaguuaaa aacagugacc gaauugcuag gcauaacaau aauggaaaga     3540

agucgauuug aaaaaaacac auccgcauuu cuagagucua aaggguaccu aaauauaaga     3600

gaugauaaac uaaugaucuu accaaaauau ucauuauuug aauuagaaaa uggcagaaga     3660

agacuucucg ccagcgcagg cgaauuacaa aaaggcaaug agcucgcauu accuacccaa     3720

uuuaugaaau uuuuauaccu cgcuucaaga uacaacgaau caaaggguaa accagaagaa     3780

auagaaaaaa agcaagaauu uguaaaccaa cauguaucau auuuugacga uauauuacaa     3840

uuaauaaacg acuucucaaa cagaguaaua uuagcagaug caaaccuuga aaaaauaaac     3900

aaacuauauc aagacaacaa agagaauaua ccuguagacg aacuagcaaa caacaucauu     3960

aaucuauuua cauucacauc acuaggugcu cccgcagccu uuaaauucuu cgauaaaaua     4020

gucgaucgua aaagauacac auccacuaaa gagguucuaa acaguacauu aauacaccaa     4080

aguauaacag gacuauacga aacccgcaua gaucuaggaa aacuaggaga agguuga        4137


<210>  5516
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5516
augacaaaug aaaaauauag uauuggccuc gacauaggaa cgagcucuau agguuucgca       60

guaguaaacg acaauaaccg gguaauuagg guaaaaggua aaaacgcaau aggaguuaga      120

uuauuugacg aagggaaagc agcggcagau agaagaaguu ucagaacgac acguagacga      180

cuauccagga gaagauggcg ccucaaacua cuacgagaaa uuuucgacgc auauauaaca      240

cccguagacg aagcauucuu uauacgcuua aaagaaagua accugagucc aaaagauagc      300

aagaaacaau acucaggaga uauauuauuu aaugacaggu ccgauaaaga uuucuaugaa      360

aaauacccca caauauacca ccuaaggaau gcccuuauga ccgaacaucg aaaauuugau      420

gugagagaaa ucuacuuagc uauacaucau aucaugaaau uuagaggaca cuuccuaaac      480

gcuacacccg ccaacaacuu caaaguaggc agguuaaacu uagaagaaaa auucgaagaa      540

cuuaaugaua uuuaucaacg ggucuuccca gacgaaucaa uugaguuccg aacagauaac      600

cucgaacaaa uaaaagaagu ccuauuagau aauaaacgau cacgugccga ccgacaaaga      660

acauugguaa gugauauaua ucaaagcagc gaagauaaag acaucgaaaa acgaaacaaa      720

gcgguagcaa cugaaauuuu aaaggcaucc cuaggaaaua aagccaaacu aaaugucaua      780

acaaacguag aaguagacaa agaagcugcu aaagaauggu ccauaacauu cgacucugaa      840

ucaauagacg acgauuuggc caaaauugaa ggacaaauga ccgaugacgg acaugaaaua      900

auagaggucc uuagaucauu auacagugga auaacuuuau cagccauagu cccagaaaau      960

cauacauuga gccaaaguau gguagccaaa uacgaccuuc auaaagauca ccuaaaauua     1020

uucaagaaac ugauuaaugg gaugaccgau acaaagaaag cgaaaaaucu ucgagcagca     1080

uacgaugguu auaucgacgg uguaaaaggu aaagugcuac cucaagaaga cuucuauaaa     1140

caaguacaag uaaaccuuga ugacagcgca gaagcaaacg agauccagac uuauauagac     1200

caagacauau uuaugccaaa acaacgaaca aaagcaaacg ggagcauucc acaucaacua     1260

cagcaacaag aauuagacca aauuauugaa aaucaaaaag cauacuaucc cuggcuagcc     1320

gaguuaaacc caaauccaga caagaaaaga caacaacuug caaaauacaa auuagaugaa     1380

uuaguaacau uccgcgugcc auauuaugua ggacccauga uuaccgcuaa agaucagaaa     1440

aaucaaagcg gggcagaguu cgccuggaug auaagaaaag aaccaggaaa uauuacaccu     1500

uggaacuucg aucaaaaagu cgacagaaug gcgaccgcaa accaauuuau aaaaagaaug     1560

acuacuaccg auacauaucu acuaggggaa gauguucuac cggcucaauc acuccuuuau     1620

cagaaauucg aaguucuaaa ugaauuaaau aaaauacgca ucgaucauaa accaaucagc     1680

auagaacaaa aacaacaaau auuuaacgac cuauucaaac aguuuaagaa cguaacuaua     1740

aaacaucuac aagauuaccu cguuucucaa ggucaauacu cuaaacgccc gcuaauugaa     1800

ggauuagcgg augaaaaacg auucaacagc aguuugucca cuuacucaga ccuaugugga     1860

auauuuggag caaaauuagu ugaagaaaac gauagacaag aagacuuaga aaagauaauu     1920

gaauggagua cuauauucga agacaagaaa auauaccgag caaaacuaaa ugaucuuacu     1980

uggcucacag acgaccaaaa agaaaaacuc gcuaccaaaa gauaucaggg auggggaaga     2040

cuaucuagaa aauuauuagu aggguuaaag aacucugaac aucgaaacau aauggauaua     2100

cuuuggauaa caaaugaaaa uuuuaugcag auacaagcag aaccagacuu cgcuaaacug     2160

guuacagacg ccaacaaagg aaugcuagaa aagaccgauu cacaagacgu cauaaacgac     2220

cuauauacua gcccacaaaa caagaaagca auaagacaaa uacuauuagu cguacaugac     2280

auacaaaaug caaugcacgg acaagcacca gcaaaaauac auguagaguu cgcucguggu     2340

gaagaaagaa aucccagaag aagcgugcaa agacaacggc aagucgaggc agcauacgaa     2400

aaaguaucaa augagcuagu cagugccaaa guaagacaag aguucaaaga agcaaucaac     2460

aacaaaagag auuucaaaga uagacuauuc uuauacuuca ugcaaggagg aauagacaua     2520

uauacaggaa aacaacuaaa cauagaccaa uuaucuagcu accaaauaga ucacauauua     2580

ccccaggcau ucgucaaaga cgacagcuua acaaaucgag ucuuaacaaa cgaaaaccaa     2640

guaaaagcgg auuccguacc aauugauauc uuuggaaaga aaauguuauc aguauggggc     2700

cggaugaaag accaaggacu gauaucaaaa gguaaauaca ggaaccugac aaugaaccca     2760

gaaaauauaa gugcacacac agaaaacggc uuuauaaaua gacaauuggu agaaacaagg     2820

caaguaauaa aacuggcagu aaauauacua gcagacgaau auggagauuc uacacaaauu     2880

aucucuguua aagcagaccu aucgcaucaa augcgagaag acuucgaauu acuuaaaaau     2940

agagauguca acgauuauca ccacgcuuuu gacgcauauc uagccgcauu uauuggaaau     3000

uaccuacuaa aaagauaccc aaaauuagaa ucuuauuuug uguauggaga cuucaagaaa     3060

uuuacucaaa aagaaacaaa aaugcgcagg uuuaauuuua uauacgauuu aaaacauugu     3120

gaccaaguag uaaacaaaga aacuggcgaa aucuuaugga caaaagauga agauauaaaa     3180

uacauacgac auagcuucgc auacaagaaa auacuuguau cucacgaagu cagagagaaa     3240

agaggagcuc ucuacaauca aacaaucuau aaagcaaaag acgacaaagg cucaggacag     3300

ggcaguaaga aacuaauacg gauuaaagac gacaaagaaa caaaaauaua cggaggauac     3360

agcggaaaaa guuuagcgua uaugacgaua guacaaauaa cuaagaaaaa caaaguuagc     3420

uacagaguca uaggcauacc cacauuagcc cuagcuagau uaaacaaauu agaaaaugau     3480

agcacagaaa auaacgguga acuauacaaa auaauaaaac cacaauucac ccacuacaaa     3540

guagauaaga aaaaugggga aauaaucgaa acgacugacg acuucaaaau agucgucucu     3600

aaaguaagau uccaacaauu aauugacgau gccggacaau ucuuuaugcu ggcaucagau     3660

acauauaaaa auaaugcuca acagcuagua aucaguaaca acgcauuaaa agcaaucaac     3720

aauacuaaua uaacagauug cccaagggac gaucuugaaa gacucgauaa ccuucgucuu     3780

gauuccgccu ucgacgaaau uguuaagaaa auggacaaau auuucagugc cuacgacgcc     3840

aacaauuuca gagagaaaau cagaaacagc aacuuaauau ucuaucaacu accuguagaa     3900

gaucaauggg aaaauaauaa aauaacugaa cuaggaaaac gaaccguacu uacaagaauu     3960

uuacaaggcc uucaugcgaa cgcaacaacc acagauaugu caaucuucaa aaucaaaacu     4020

ccuuuuggcc aacuacgcca aagaaguggu auuucgcugu ccgaaaacgc acaacuaaua     4080

uaucaaucac caacagguuu auuugaacga cgaguucaac uaaacaaaau aaaauga        4137


<210>  5517
<211>  4137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5517
augacaaacg aaaaauacuc aaucggauua gauaucggaa caucgucgau uggauucgcg       60

gucgucaaug acaauaaucg aguaauacga gucaaaggca aaaacgcaau agggguacga      120

uuauucgacg aaggcaaagc agccgcagac cgacgcaguu uucgaacaac aagacgacga      180

cuaucaagac gaagauggag guuaaaauug uuaagagaaa uauucgacgc auacaucacu      240

ccaguggacg aagcauucuu uauccgucua aaagaaucaa auuuaucucc aaaagacagc      300

aagaaacaau acucagggga cauauuauuu aaugaucgau cagacaaaga cuucuacgaa      360

aaauauccua caauuuauca cuuacgcaau gcacuaauga cagaacauag aaaauucgac      420

guaagagaaa uuuaucuagc uauccaccac auaaugaaau ucagaggcca cuuucuaaau      480

gcuacaccag caaauaauuu uaaaguagga agauuaaacc ucgaagaaaa auucgaagaa      540

cuaaacgaca uauaccaacg gguauuccca gaugaaucua uagaauuuag aacagauaau      600

uuagaacaaa uuaaagaagu acuauuagac aauaaacggu cacgagcaga ucgacaaaga      660

accuuagugu cagacauaua ccaaucuuca gaagacaaag acauagaaaa acguaauaaa      720

gccguagcaa cugaaauauu aaaagcauca cuaggaaaca aagcaaaauu aaaugucauu      780

acuaauguag aagucgacaa agaagcagca aaagaaugga guaucacauu cgacagcgaa      840

aguaucgaug augacuuagc aaaaaucgaa ggacagauga cggacgaugg acacgaaauc      900

aucgaaguuc ugagaucucu auacuccgga auaacucuuu cagcgauagu accagaaaau      960

cauacacugu cacaaucgau gguagcaaaa uacgacuugc acaaagacca cuuaaaauua     1020

uuuaagaaau uaauuaacgg aaugacagac accaagaaag ccaaaaacuu gcgcgcagcu     1080

uaugacggcu acauagacgg cguuaaagga aaaguacucc cucaagaaga cuucuauaaa     1140

caaguacaag uaaacuuaga cgauucagca gaagcaaaug aaauacaaac auacauagac     1200

caagauauau ucaugccaaa acaaagaaca aaagcaaacg gaagcauccc ccaccaacuu     1260

caacaacaag aacucgacca aauaauagaa aaccaaaaag cguacuaucc uuggcuagcu     1320

gaauuaaacc cgaauccaga caagaaacga caacaauuag caaaauacaa acuagaugag     1380

cuaguaacau ucagaguacc auacuacgua ggucccauga uaacagcaaa agaucagaaa     1440

aaucagagug gagcagaauu ugcuuggaug auaagaaaag aaccagguaa uaucacuccc     1500

uggaacuucg aucaaaaagu cgacaggaug gcgacagcca aucaauucau aaaacgaaug     1560

acgacaacag auacauaccu auuaggagaa gauguacuac cugcccaauc cuuauuauau     1620

caaaaauuug aaguacuuaa cgaacuuaau aaaauucgca ucgaccacaa accaauauca     1680

auugaacaaa aacaacagau auucaacgac cuauuuaaac aauuuaaaaa cgugacaauu     1740

aaacauuugc aagacuaccu uguaagucag ggacaauaua guaaaagacc ccuaaucgaa     1800

ggguuagcag augaaaaacg cuuuaauagc agucucagua cauauucuga uuuaugugga     1860

auauuuggag cgaaauuggu agaagaaaac gauagacaag aagaccuuga gaaaauuauu     1920

gaauggagua caauuuucga agauaagaaa auuuacaggg cgaaauuaaa cgauuuaaca     1980

ugguugacag augaucaaaa agaaaaacuc gcaaccaaac guuaccaagg cuggggaaga     2040

cuuucuagga aacuccuagu aggucucaaa aauucagagc accgcaauau aauggacauu     2100

cuauggauaa cuaaugaaaa uuucaugcag auccaggccg aaccagauuu cgcaaagcua     2160

guaacagacg ccaacaaagg caugcuagag aaaacagauu cacaagaugu cauuaaugau     2220

cuuuacacga guccacaaaa uaagaaagcc auacgccaaa uacuucucgu aguucacgau     2280

auacaaaacg caaugcacgg acaagcgcca gcaaaaauac auguugaauu ugcaagagga     2340

gaagaaagaa auccacgaag auccguacaa agacagcgcc aaguagaagc agcguaugaa     2400

aaggucagca augagcuagu aagugcuaaa guaagacaag aauuuaaaga agcaaucaac     2460

aauaaacgag acuuuaaaga uagauuauuu uuauacuuua ugcaaggagg aauugauauc     2520

uauaccggua agcaacuaaa uaucgaucaa uuguccucau accaaauaga ucauaucuua     2580

ccacaagcau ucguuaaaga ugauagccua acaaacagag uacucacaaa cgagaaccag     2640

guaaaagcag auucuguacc aauugauaua uuuggcaaga aaaugcugag cguuuggggg     2700

cguaugaagg aucaaggccu gaucucaaag ggaaaauaua ggaaccuaac caugaacccc     2760

gaaaauauau cugcacacac cgaaaacggg uucauuaaua gacaacucgu agaaacacga     2820

caagucauca agcuagccgu caacauauug gcagaugaau acggagacag uacacaaauu     2880

auaucaguua aagcagauuu aucacaucaa augagagaag auuucgaacu acuaaagaac     2940

cgcgacguca augauuauca ccaugcauuc gacgcauauu uagcagcguu uaucggcaau     3000

uaucuuuuga aaagauaucc aaaacucgaa agcuacuuug uuuauggaga uuucaagaaa     3060

uucacgcaaa aagaaacuaa aaugaggcgc uucaauuuua uauacgaucu caaacacugc     3120

gaccaaguag uaaauaaaga aacaggugaa aucuuaugga caaaagacga agacaucaaa     3180

uauauaaggc acuuauuugc uuauaagaaa auccucguau cgcacgaagu aagagagaaa     3240

aggggagcau uauacaauca aacgauuuac aaagcaaagg acgacaaagg aagcggacaa     3300

ggcagcaaga aauuaauucg aauaaaagac gacaaagaga caaaaaucua cggaggauau     3360

aguggaaaau ccuuggcaua uaugacgauc guccaaauaa ccaagaaaaa uaaaguaucc     3420

uacaggguaa uaggaauacc cacgcucgcc cuugcccgac uaaacaaacu cgaaaaugau     3480

aguacagaaa auaauggaga acuauauaaa auaauaaaac cacaauucac acacuacaaa     3540

guagacaaga aaaauggaga aauuauugaa acaacagaug acuucaaaau ugucgugucc     3600

aagguaaggu uccaacaauu aauagacgac gccggacaau uuuucaugcu agccucagau     3660

acauauaaaa acaaugcaca acaguuaguu aucucaaaua augcguuaaa agcaauaaac     3720

aauaccaaua ucacagauug uccacgagau gaccuagaaa gacuagauaa ccuacgucuu     3780

gacucagccu ucgaugaaau agucaagaaa auggauaaau auuucucagc uuacgacgcu     3840

aacaauuuca gagagaaaau acguaauagu aaccuaauau uuuaucaacu accaguagag     3900

gaccaauggg aaaacaacaa aauaacagaa cucggcaaac gcacugucuu aacucgcauu     3960

cuucaggggc uucacgcaaa cgcaaccaca acugacaugu cuauuuuuaa aauuaaaacu     4020

ccauuuggac aauuaagaca acgaucagga auaagccuuu cugaaaaugc ucaauuaaua     4080

uaccaaucuc caacaggauu auuugaaaga agaguccaau uaaauaagau uaaauga        4137


<210>  5518
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5518
augaaacaaa uauuaggccu cgaucuagga acaacuucaa uaggaugggc auugguucgu       60

gaagcugaaa acaaagauga acaaucauca auaguaagga uugggguucg aguaaauccg      120

cugacaucag augaacaaaa aaauuucgaa caaggaaaaa gcauaacaac aaaugcugac      180

agaacacuua aacguaguau gagaagaaac uuacaacgau acaaacuaag acgugaucau      240

cuaaaagauu gccuacaaaa aaaugggaua auaaccgaua aagcaguauu aucagagaac      300

ggaaacuaca caacguucca aaccuacuau uuaagagcaa aagccguaaa cgaaaaaaua      360

ucauuagaag aauuugcacg aguucuacua augcucaaca agaaaagagg auacaagagc      420

ucaagaaaaa ugaaaucugc agacgagggu caauuaauag acggcauguc uguagccaaa      480

agacuuuaca augacaauau uacgccaggu caauauguac ugucucuacu cgaagaaggu      540

agaaaauaca uaccggauuu cuacagguca gaucucuuag acgaacuuga uaaaauaugg      600

acguuucagc aaugcuuuua cccagaaaua uuaaccccug aguguagaaa acuuauagca      660

gguaaaggua aacaagcaac agcaaaaauu uucuugggaa aauauaaaau auauacugca      720

gacaacaaau ccaaagacaa acgauuacaa gcuuaccaau ggaggguaga agcacucuca      780

ggacaacuuc caauagaagu aguagcauuu gucgugugcg agauaaacgg ggcuauccaa      840

gcauccagcg guuaccuagg agcaauuucu gacagaagca aagagcuaua uuuugaagga      900

cucacaguag gacaauaccu agucaaacag cuugaaaaaa aucccgacac uucacucaaa      960

aauaaagucu ucuaccggca agauuaucua aaugaauucg aaaaaauaug ggaaaaacaa     1020

gcagaauacc acacagaacu gacaccgcaa cuaaaaaaag aaguaagaga uauaauaauu     1080

uuuuaucaaa gaacgcuuaa aucacaaaaa ggucucguag guuucuguga auuugaaagc     1140

cgccaaauag aggugaugau agauggaaaa cuuaagaaaa agacaguugg cugcagagua     1200

ugcccuaaau caucaccuuu auuccaagaa uauaagauau ggcauaaccu aaauaaccuu     1260

aaaauaguag aaaaagaacu ugggaaaucu agauuccuca aucaagacga aaaagaauca     1320

cuauuccaag aacuaaacau aaaggaaaaa uuauccaaaa cagacauacu aaaacuccua     1380

uguuuaaaag caaaagaauu cgauuuaaac uuugagaaag uagaagggaa ucgaacucaa     1440

gcagaauuau ucagagccua ccaggcuaua auagaaaugu cagguaacgg cgauuacgac     1500

uucucaaaaa ugacgucaag cgagauccaa gauacaguca cagguguuuu cagaggauua     1560

ggauacaaua cagacauacu auccuuugac gcagaaaaag aagagaaaac auuugacaaa     1620

caaccgaugu auuuauugug gcaccuacua uacucauacg agggagauuc aucagauaca     1680

ggcaacaaag cccuaaucac uaaaauuaaa aaauuaugug gauuugaaag ugaggaauau     1740

gcaagaauac uuacugacgu uacauuccaa ucggaauaug gaaacuuaag uaccaaagcc     1800

aucagaagaa uacuuccuuu caugaaaaaa ggaagugaau auagcgaagc augccgacau     1860

gcaggauacc gucauucaga aagaagccua acaaaagaag aguuagacaa aaagguuuac     1920

aaagaccacc uagaauuguu accaagaaac ucacuacgaa auccaguagu agagaaaauu     1980

cuaaaccaaa ugauacacgu agucaauaca guaguggaua cuuauggcaa accagacgaa     2040

auucggauug aauuagcacg ugaacuaaaa aaaucagcaa aggagagaga cgaaaugaca     2100

gcauccauaa acaaaacagc agcagaacac aaaaaauacu gugaagugcu ccaaaaagaa     2160

uucggauuca cgcacgugag uagaaacgau auuauaaggu auaaacuaua ucuagaacuu     2220

gaaaauaacg gauauaaaac acuauacuca gauaccuaua ucccacgaga aaaauuauuc     2280

ucuaaggaau ucgauauaga acauauaaua ccacaaucaa aguuauucga cgacuccuuc     2340

ucaaacaaaa cguuagaaug ucgauccgua aauauagaaa aaaguaauga uacugcauau     2400

gauuacguac uuaacaaaua uggugaacaa ggaauauuag auuauaaaaa caggaucgaa     2460

aauuuauaua aggacucuaa gauuaguaga accaaauacc aaaaauuaau gaugaaaaac     2520

agugauaucu ccggaaauuu cauagaaaga gaauuaagag auagccaaua uauagcaaaa     2580

aaagcaaggg aaauuuuaga agaauuagug aaauucguag uuccaaccac aggcucagua     2640

acagcaagau uacgagaaga cuggcaacua gucgacguaa ugcaagaacu uaauuggaag     2700

aaauacgaca aauuagggcu aacagacgua cguuaugaca aagauggcag agagaucaga     2760

uacauaaaag acuggucaaa aagaaaugac caucggcacc acgcaaugga cgccuuaaca     2820

auugcauuua ccaagagaag caucauccaa uaccuaaaua acuuaaaugc aaaaagugac     2880

aaaucugguu caauauaugc gauagaacaa aaagaacuuu uucgcgacaa aaggggcaag     2940

cuuaaauuua uaccucccau gccauguaau auauuuagag cagaagcgaa aagacaucuu     3000

gagaauguuu ugguaucugu gaaagcaaaa aacaaagucg uuacaccaaa uaucaaccga     3060

agcaaaggaa aaaacggaau caauaagaaa auucaacuaa caccacgugg acaguuacac     3120

aacgagacaa ucuacggcuc gaugaagaga uacgaaacga agugggaaaa aaucggaagu     3180

ggguucgaug agcgacaaau ugcuaaagua gcuaacaaaa aauuuagaga agcauuauua     3240

ucaagacuag cagaauucgg caaugaccca caaaaagcuu uuaccggaaa aaacagucug     3300

gagaagaauc cauuguaugu cgaugaguua cacacucaga aaguuccgga aaaagucaaa     3360

auaguaacuc uagaaaacuu auacacaaua cgcaaggaaa uuacaccaga ccugaagauc     3420

gauaaaguag uggaugcagg ggucagacga guacuacaag aaagacuaga cgaauucgac     3480

gacgauucaa aaaaagcauu uucuaaucua gacgaaaacc caauaugguu aaacggaaaa     3540

aagaugaaac ggguaacaau aaugggcgua uccaacgcag uaccuuugca cgacaaaaaa     3600

gauuacaaag gcaauuuagu auucgauagc aaagggagga agcaaccagu agauuuugua     3660

aauaccggaa guaaccacca uguugccaua uaccgagaug aaucaggaga acuacaagac     3720

auuguaguaa gcuucuauga agccacaaca cgaucgacuu uagggauccc cguaauagau     3780

aaagaauaca aaaaagcaga aggauggaac uuucuuuucu caaugaaaca aaaugaauac     3840

uucguauuuc cgaaugagga aacagguuuu aauccuaaag aaauagauuu aaugaaucca     3900

gauaacuaua cuacaauauc uccgaaccuu uaucgagucc aaaaauuuuc caaauguaug     3960

uauggaaaua gcagugugcg agaauacguc uuuagacauc accuagaaac aagcgugaau     4020

gacaguaaag gauuaaaaaa cauugcauac aaaaauguaa aaaguuuagu auaccucgaa     4080

ggaauaguca aaguaaggau uaaccauaua ggacaaauag ugagcguagg agaguacuga     4140


<210>  5519
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5519
augcgaauag auaacggacu cgaagaauua aaaguaaaca acagaaucua uaauaucggc       60

uuagauaucg guacaucuuc agucggauuc gcuguaacag acgcccagga uaaauuaguu      120

agagucaaag gaaagacugc uauaggcgua cggcucuuug aagaaggcca uaccgccgca      180

gaacggagag gcuucagaac aacacgaaga agauuaucac gcagaaaaug gagaauaaac      240

uuacucaauc aaauauucga accauacaua agcgaaauug acccuacuuu cuuugccaga      300

uugaaaaaua gcagcuuagc cgguaaagau aagaaauuug aaggaucucu cuuauucccg      360

gaucgaacag auuaucaauu cuauaaagac aacccgacca uguaccauuu acgcaaacac      420

uuaauggaaa aagacagaca auuugaucug cgagaaauau accucgccau gcaccauauu      480

auaaaauaca ggggaaauuu ccuaaauaca acaccaauga accacuuuaa aaaugaagaa      540

aucgacuuua gcgccuuauu uucuagacua aaugaacuuu accaagaaau cgacgacgaa      600

aauaucuuug aacucaauau agagaauguu gaugaaauua agaaaauauu auuaaaccac      660

gaccuguuca aaauggaaaa acagaaacaa guugcgaaac uuuuaaaugu ugacaccgau      720

gacaaacaac uaaagaaaga aaauacugcg uuagcaagca auuucagcaa agcaauucua      780

ggauacaaau ucaacuuagc ucuaguaaca aaaauaccug aucaagacaa gaaagaaugg      840

acaauuucac uaggagacga agacuucgau gacagauuag acaacguacc aguagcacuc      900

accgaaucac aacuagaagu cguagaggua uuacauucga uauucaacuc aauaucacuc      960

aaugaaauag ucgaaaacgg agaaacacuc uccagugcaa ugguaagaaa auacaacgau     1020

cauaaacaug acuuaaaacu ucugaaaaau guaaugacga cucucgaaga uagaaagaaa     1080

gcuaagaaaa uacaagguau auacaacguu uuuguuggua aagauacuga ccuaaucucg     1140

gauaaagaca aaaacaaaua caaagacaaa gaugaguucu auaaagaaau acagaaaaac     1200

uuagacaauu ccagcgacgc cgaagaaauu auaaaaagaa uaaaacaaga ccaauucuua     1260

cccaaacaac gaaccagcaa aaauggagua auaccccacc aacuccauca aagagaacua     1320

gauuuaauua ucgaaaacca agcaaaauac uacccuuuuc ucaaagaagc aaacccuaac     1380

aagaaacgac uuaaacaagc caaauacaaa cuggaugaac uuaucgcauu uaaaauccca     1440

uauuauauug gcccaaccag uggagcugaa aauucggauu uuucauggcu caaacggaag     1500

aaagacgggc aaauuacacc auggaauuuu gaugaaaaag uagaccgaau cgaaucagcc     1560

aaccgauuca ucagacgaau gacgacuaaa gacaccuacc uucucgcaga agacguacuu     1620

ccauuagaau ccuugaucua cgagaaauac ucaguccuca augaguuaaa uaacguccga     1680

aucaauagac aaaaacuauc aguaaacgau aaacaagaua uuuacaauga acuauucaaa     1740

aacucuaaaa caguaacacu aaaacaauua caaaaauacu ucgaagucac aaagaaauua     1800

aaaacggucg ucgucgaagg cuuagcagac ccgaagaaau uucugaguaa cuuagguaca     1860

uaucaugacu uuaaauuaau auuuggagac aucgucgacg accccgaaua ucaaacagac     1920

cuagaaaaca ucauagaaug gagcacuauu uuugaagaua gaggaauauu uaauaguaaa     1980

uuaaacgaaa uaacuugguu aacagacgaa caaaggaaga aaguucuauc aaaaagauau     2040

caagguuggg gcagauucag caagaaacua uuaguuggac uuaaaaacuc ucaaggcaac     2100

ucaauacugg acgaauuaug gaacacaaau cucaacuuua ugcaauuaca aagccaagac     2160

gacuuugcug aauuaaucca cgcagaaaau gcuaaacaau uuaaagcaaa cgaucccgac     2220

aacccaugga acggaauaga cgcuauacua aaugaugcau acaccagucc acaaaacaag     2280

aaagcgauaa ggcaaguagu caaaguagua caagauauag aaaaagcuau gggaaauaaa     2340

ccacagaaaa uagcaauaga guucacccga agcgccgaca aaaauccuag acguaccgau     2400

agcagaauaa aaagauuauc agauauuuac aaagaagcag cagaaaaagu aguaggagaa     2460

ucgcuaaaau uagaauuaaa cgaauaucua accucuaaaa agaaaaugac agacaaaaug     2520

uaccuauauu ucacccaacu cggaaccgau aucuauaccg guaagaaaau agauaucgau     2580

caacuaaaca auuaugauau agaucacgua uuaccccaga gcuuucuaaa agaugauucc     2640

uuagacaauc gaguccuuac caacagaucc gauaauagcg aaaaaucaga cacagucccu     2700

uuacacaaau uuggcgcaaa aaugggcaac uucuggagac aacucgcuga cgaaggauua     2760

aucacaaaaa ggaaauacaa aaaucuuacu accgaccccg cuacaauuga caaauauacu     2820

auacaaggau uuauucgacg acaacuuguu gaaaccagcc aagucaucaa auuaacagcg     2880

aauauauuag gcggccuuua cccagaagaa acagaaauuc ucgaaauucc cgcaaaacua     2940

aaccaccaau uuagagauau guucgaccuc guaaaagugc gugaagugaa ugauuaccac     3000

caugcauuug acgcuuacau cacaguuuuu guaggaaauu uucuauaccg aagguacccc     3060

aaauuaagaa guuacuuugu auacggagac uuuaagaaac uuaauaaaac cgacgacgau     3120

auuagaaaau ucaauuuccu caacuuauuu aacaaagaua aaguugaaga uaaugaaaca     3180

gaugaaaucu uauggagcaa agccgaauca auucgagaca uuaaaaacau auauaauuau     3240

aaauuuaugc ucguaucacg cgaaguauau acacgacacg gugcuauguu caaucaaacu     3300

auuuacccag ccucuaaaaa ugauucaaag aaacuaaucc caauuaagaa aggaaaagac     3360

acugcaauau acggaggaua uagcgguaac accgacgcau acauggccau aguagcagua     3420

gaagacaaga aaggagaaaa auuuaaaguc gucgguguac caacuagagc aguaagcaga     3480

cuugaacaac uagcugcaga cgaucaugac caauaccuaa cagcacugca cgacguucua     3540

aaaccaaauu ucaccaaaau caaaacaaau aggaaaacaa aagccaaaga agaagucacu     3600

acaccuuucc gaauacuugu aggaaaagug ggauaccgac aacuaauaca agacggccaa     3660

guaaaaauga uguuagggag uucugccuau aaauacaacg ccaaacaauu aguacuaagu     3720

gagaaaucau uaaaaguuau caaaaacaau aagaaauaua acccugauga ugaaaaucaa     3780

gaccuaauag acguauauga ugaaauauua gguguaguca acagggccuu caaucucuac     3840

gacacaaacg gauuuagaaa gaaacucaau gauaaccgag ucaaauuugu ugaacuccca     3900

auuaauaaca uauuugacgg aacaaaauua guacaaaugg gaaaaagagc aguauuaaca     3960

caaauucuua acggacuuca cgccaaugca gcauuaggug acuuaaaagc guuaggaauc     4020

agcacaccau ucggcaaaau gcaaaguaaa uccggaaucc cacuauccau ggaugcaagc     4080

cuaguauacc aaagcccaac uggccuauuc gaaagaaaac uaaaccuuag ugaccuauga     4140


<210>  5520
<211>  4140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5520
augacuaaga aaaauuacuc cauaggauua gauauuggaa caaacagugu uggaugggcu       60

guaauaacag acgacuacaa agugccagcc aagaaaauga aaguacuagg gaauacagau      120

aagaaauaua ucaagaaaaa uuuacuagga gcacuucugu uugacucagg agaaacagcu      180

gaagcaacaa gacuuaaaag aacugcucgu cguagauaua cgcgaaggaa aaaccgacuu      240

agauauuuac aagaaaucuu ugcagaugaa auggcaaaag ucgaugagag cuucuuucaa      300

agauuagacg aauccuuccu ucgaugggac gacgauaaua agaaauuggg acgauauccc      360

aucuuuggua auaaagccga uguuguuaaa uaccaccaag aauuucccac caucuaucau      420

cuaaggaaac auuuagcuga cagcagcgaa aaagccgacc uacgucuagu auauuuagca      480

uuagcgcaca ugauaaaauu ccgaggacau uuccuaauag aaggagaacu aaacgcugaa      540

aacacagacg uacagaaaau uuucagugac uucgucagag uauacgauag aacguucgau      600

gacucucauu ugucagaaau uacagucgac gcagcaucaa uacuaacaga gaaaauauca      660

aaaaguagaa ggcuagagaa cuuaauuaaa uacuauccaa cugaaaagaa aaacacacua      720

uucggaaacu uaauagcacu agcucuaggc cuccaaccca acuucaaaau gaacuucaaa      780

cucagcgaag augcuaaacu acaauucucu aaagacuccu auaacgaaga cuuagaagaa      840

cuauugggaa aaauagguga ugauuaugca gaucuauuca cgagcgcgaa aaaucuauac      900

gacgcuauau uacuaucagg cauacuaacu gucgacgaua acagcacaaa agcgccccuc      960

agugcaagca ugauuaaaag auacgcugaa caucacgaag aucuggaaaa auuaaaagaa     1020

uuuauuaaag caaacaaaag cgaacuauac caugauauuu ucaaagauga aaccaaaaac     1080

ggcuacgcag gauauauaga aaacggcguc aaacaagacg aauuuuauaa auaucucaaa     1140

aacacguuau cuaaaauagc aggaagugau uacuucuuag auaaaauaga acgugaagau     1200

uuucuaagaa aacaaagaac auucgauaac ggcucuauac cacaucaaau acaccuccaa     1260

gaaaugcacg caauccuuag aagacaaggc gacuacuacc cguuuuuaaa agaaaaccaa     1320

gauagaauug agaaaauacu cacuuuccga auaccuuacu augucggacc auuagcaaga     1380

aaagauagca gauuuucgug ggccgaauau cauucagacg agaaaauuac cccauggaau     1440

uucgauaaag uuaucgacaa agaaaaaucg gcagaaaaau uuauaacaag aaugacacua     1500

aacgauuuau accuuccgga agaaaaagua cuuccaaaac acucacacgu uuacgaaacc     1560

uacgcaguau auaacgaacu cacaaaaaua aaauauguaa acgaacaagg caaagauagc     1620

uucuuugaca gcaauaugaa acaagaaauu uucgaccacg ucuucaaaga aaacagaaaa     1680

guaacaaaag aaaaacucuu aaauuacuug aacaaagagu ucccagaaua cagaauuaaa     1740

gaccucauag gacuagacaa agaaaauaaa ucauuuaacg caagccuagg aaccuaucac     1800

gaucuuaaga aaauacuaga uaaagcguuu cuagacgaca aagucaacga agaaguaaua     1860

gaagauauaa uaaaaacuuu aaccuuauuc gaagauaaag auaugauaca cgaacgacug     1920

caaaaauauu cagacauauu caccgcugac caacuaaaga aacuagaaag aagacauuac     1980

acaggauggg gacguuuauc guauaaacug auaaacggaa uaaggaacaa agaaaacaac     2040

aaaacuaucu uagauuaucu aauugacgac ggcucagcua auagaaauuu uaugcagcua     2100

auaaacgacg acacauuacc cuucaaacag auaauccaaa aaucacaagu aguaggagac     2160

guugaugaca ucgaagcggu agugcaugac cuucccgguu caccggcaau caagaaaggc     2220

auucuucaau cuguaaaaau cguugaugaa uuaguaaaag uuauggguga uaauccugau     2280

aacauaguaa uagaaauggc gcgugaaaac caaaccacaa auagagguag aagucaaagc     2340

caacaaagac uaaagaaauu acagaacuca cucaaagaac uuggaucuaa uauacucaac     2400

gaagaaaaac ccucuuauau ugaagauaaa guugaaaacu cacauuuaca gaaugaucaa     2460

cucuuccuau auuacauaca aaacgguaaa gauauguaca cgggagauga acuagauauc     2520

gaucaucugu cagauuauga cauagaucac auaauaccac aagcauuuau caaagacgac     2580

uccauagaua auagaguacu cacaaguagc gcuaaaaaca gagggaaaag ugacgacguc     2640

ccaaguuuag auauaguacg agcccgaaaa gccgaauggg uaagacuuua caaaucaggc     2700

uuaauaagua aaagaaaauu ugauaauuua acgaaagccg aaaggggagg uuuaacagaa     2760

gcggauaaag cgggcuuuau caaacgacag cuagucgaaa cuagacaaau aacaaaacau     2820

guugcacaaa uucuagaugc aagguucaau acggaauccg augaaaacga uaaaguaauu     2880

agagauguaa aaguaaucac auuaaaaagc aaccuaguau cacaauuucg uaaagacuuc     2940

gaauuuuaca aaguaagaga aauaaacgau uaccaucacg cacacgacgc auauuuaaac     3000

gcgguaguag gcaccgcauu acucaagaaa uauccaaaac ucgcgaguga auuuguauac     3060

ggugaauaua agaaauacga uguacacaaa uuaauagcaa aaaguucaga cgaucauucc     3120

gaaaugggaa aagcaacagc aaaauacuuu uucuacagca accucaugaa cuuuuucaaa     3180

agaguaaucc gauacucaaa cgguaaaguc aucguacgac caguugugga auacucaaaa     3240

gacacagaag auauugcaug ggauaagaaa ucaaacuuca ggacaauaug uaaaguauua     3300

ucuuacccac aaguaaauau ugucaaaaag guagaaacac aaacuggcgg auucucaaaa     3360

gaaagcauac uuccuaaagg ggauucagac aaacucaucc cacguaagac caagaaagca     3420

uacugggaca cuaaaaagua uggagguuuu gacaguccaa caguagcaua caguguauuu     3480

guaguagcag auguagaaaa aggaaaagcc aagaaacuaa aaacaguaaa agaacucgua     3540

ggaauuucca uaauggagag aucuuucuuu gaagaaaauc caguagaguu ccuggaaaau     3600

aaagguuacc auaauauaag agaagauaag cuaauuaaac uaccaaaaua cucucuauuc     3660

gaguucgagg gaggcaaacg uagacuauua gcaagugcau cagaauuaca gaaaggaaac     3720

gaaaugguua uacccggaca ccucgucaaa cuacuauauc acgcacaaag aauuaauuca     3780

uucaauagua caaaauauuu agacuacguu ucugcccaca agaaagaauu ugaaaaagua     3840

uuaucaugug uggaagacuu cgcgaaucug uaugucgaug uggagaaaaa ccucagcaaa     3900

auccgagcug uagcugauuc aauggacaau uuuuccauug aagaaauauc aaacaguuuc     3960

auuaaucuau uaaccuuaac agcgcuggga gcccccgccg acuuuaacuu ccuaggagag     4020

aaaaucccca gaaaaagaua uacgaguacg aaagagugcc uuaaugcuac auuaauacac     4080

caaucaauaa cgggauuaua ugaaacuaga aucgaccucu cuaaaauagg agaagaauga     4140


<210>  5521
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5521
augggaaaau acaaccuugg acucgacaua ggcacuaauu caguaggcug ggccguaguc       60

gacgaaaaca accaaauagu gaaaaaagga ggaaaagccc ucuggggggu cagaauguuu      120

gacgaagcgc aaucagcuaa agaaacacga auauacagga acagucgacg aagaaucguc      180

cgacgaaauc aaagacuacu cuuauuacaa aaagaauucu acgacgaaau aauaaaagua      240

gaccaaaccu ucuuucaacg ccuaaaagac agcuucuuua aaaaagaaga caaauuaaac      300

aaaaauaauu auaccuucuu ugaugaugac auuacagaua gagaauacuu caaaaaauuc      360

cccacaauau aucaccuaag aucacauuua cuauacaaug acgaaaagau cgacauuaga      420

auguuauauc uagcccucca ucacaucguc aaauaccgug gacacuuccu cgacuccggc      480

gaggucaaug aaucaaacuu cagcaagaua aaagacgucc uagauacauu uaauaauaaa      540

augagcgaaa ugucacucga auuugaagac aacgaagacu acuucuguaa aauagaauuc      600

gacgaaaauu uugagaacaa auuaaaagau aucaugcucu cgcaacucag caaaaaagaa      660

aaacgacaag aacuaaucga uuuauuuaaa guagacaaaa agucauuagu caacgaacua      720

cuaaucgaac uccuagucaa caaaaaagua aaacuaaacu ccuuaaguau caccaacggu      780

aaaaaauacg aaguauguga aauagacuuc gaaaaagaag aacucuucga aaaauuagaa      840

gaugcgaaga aaauaauaac cgaauuauuc gacauccucg acgaaauugu uaauauaaaa      900

aaaauaguug acuauuauua uguacucaaa auaauaggag acaacgaaua cuuauccgaa      960

gcaaugguaa aaaaauauga caaacacgac aaagacuuaa gagaacucaa aagcuucauc     1020

aaaaccuacu gccccaauaa auacuaugaa uguuucagaa aaacgucuaa ccccaaucuu     1080

ugcaauuacc ccaaguaugu cggaauaaac ucugucaaca aaaaaauaga acguuucgca     1140

cacuguaaaa aagaagaauu uuauaaauau cuuaaaucuu uguucaauac aauaaaagau     1200

gagaacgcaa aauuaaaaau cgacuuauuc caaaaagaua uggaaaauga cgauuuccua     1260

aauaggcaaa acagcaacca caacagcagc aucccaaugc aacuaaacuu uugugaacuu     1320

aaaaaaauac uagaaaacca aucuaaauau uacccauuuu uaaugcuaaa aaacuccgac     1380

ggcuacucca cauccgacag aauaaucgca aucuucaaau acaagauacc auacuauguc     1440

ggaccccuua acaagaacuc caaauacagc uggcuuguaa aaaaaaguaa cgaaaacaua     1500

accccuugga acuacgaaaa gguaauagau uuagaugcgu ccgcaaaaga guucauacaa     1560

agaaugcaaa acaaaugcac auaccuuaaa ggggaaaaug acuacugucu ucccaaaaaa     1620

ucaauacuau uuucagaaua caacugccug caauacuuaa auagacuaau aauaaacggg     1680

gcacucaucg aaaacaaacu aaaaaacgag auauaugaaa acaucuuccu cuguuauaaa     1740

aagccaacaa aaaagagcau aguugaauac cugaaaacca auuacaacua ugacgaaacc     1800

accuugaaaa gcaauauacu cgaagugaau ugugacaugu cgaguuauau ccaauuuaaa     1860

gaaaucuacg gugaggacuu cgaacaaaac cuaaacauag ucgaagacau caucaaagac     1920

auaacuauau uugaagacaa aaaaauauua gaaaagagac uacgagaaau auaucaucua     1980

uccgaagaca agguacaaaa gauaaaaggg cucaacuaca aaggcuacgc uacccuauca     2040

aaacacuuuu ugaauaauuu cuacuccgua aacgaagaga ccggagagau uuacggacca     2100

aucauacaaa uaaugcgaga cacaaacuau aaucugcaac aaguaauuua caacgaccga     2160

uauaauuuca ugaaagcaau agacaaauac aauaaaaaaa acuuagacac agaaaaaaaa     2220

cucaacgugu cugauuuuau agacgaguau auauauguau cuccgauaau gaaaagaagu     2280

cucauacaau ccuacaacau cauacaagaa cuggaaaaaa uccuaggcga gucuauuagc     2340

aaauacuaca uagaaugugc aagagugcgc agcaacucua aggacguuau caauagcaga     2400

uaucgacaac uauccgaacu auacaaagac ugugaacacc aaguaucuga cauaagcaag     2460

aauuacauua acauaaauaa auuaaaaaaa caacuagaag acaauaagaa cgaacuaagg     2520

gcagacaaac uuuaccucua cuucacacaa uucgggaaau gcauguacac acuaaaagac     2580

aucgaccucg acgacgucuu cuugggcaaa uaugacauag accacauaua cccccaaagc     2640

uuaauaaaag augacucacu uaacaacaga guucuugcaa gcaaagaaaa aaacggaaaa     2700

aaagacaauc aauuuauauu uaacauccca gaauuccuuc ccaaaaaugc agagcucuuc     2760

uauaagaaac uccuugaaug caaguuaaua acuaaagaaa aguaucgcag acuaaccaug     2820

aaaaaaauag acgacgauga acuagacaua uucguaaaua gacaacuagu agcaacgaac     2880

caaucaguca aaggauuagu ggacguacua aaaauauaca aaggaguaaa gccaacuaau     2940

auagucuauu cgaaagcugu caacauaucc gacuucagaa auaauuauaa ccuauauaaa     3000

ucaagaacag caaacaauua ucaucaugcu caugaugcgu auuuaaacgu agucauugga     3060

aaagcccuug acguauacua ucaaugcaac uacuuccaaa auuauaagga cgucaucaaa     3120

cuaaaagaca ggaacauuac cauaaaccca gaaauaauac uaucaaaaaa uagauacgua     3180

aauagaaaug gcgagaaaaa aguaauuugg gaccucgaua acacacuaaa acuaauaaaa     3240

aauaauuuau acaaucguuu ugacauaacc gaaacaauua ugacaagaaa aucuaauaaa     3300

auguuuucaa aaagcuccau cucaccugcu aaaaauucaa aaaaucuaau uccgauaaaa     3360

accuccgauc cccgauguaa cgucuuaaaa uauggcggaa uaaaaucaaa uuccuacaac     3420

uuuuauugca uaguugagcu cuacgacaag aaaggaaaga aaucauacau ccuagaagca     3480

aucccaaaga cucacucaga uaaguuggaa gaauauauaa agaauucuau agacgaaaaa     3540

aacguaucaa auuucaaaau aauaaacaaa aauauuaaaa guaauauaau aauagaauau     3600

aaaaaacugc gcuacguaau aaccggcaaa uccggaaaau acuaucuaau uaaaaacuuc     3660

aaagaccgga auuuuucaua uaacgccauc aaaaccauaa aauauauuga gaaauacaac     3720

uaccaaaaaa aauacgaaau uccauuugaa gagacuaaag aaaaaauaau auuagccccc     3780

aaaaaaaaag acacgaaaga aauuucacua acccuagaca acugucaggc acuacucgau     3840

gaaauaauuu cacuguucaa aaaagacaua uauaacuaug acaucauaua caaaaucacc     3900

agcaaacuag aagaaaauaa acagaaccua acgcuuacaa accuaaucaa aguguuaacu     3960

gaaauuuuaa uacucuuaaa aacaaaugaa cgcggaacag cagaccuaac acuuauagac     4020

augagcaaag auacaggaac acuaugcauc uccaaaacau uaaaaccagg uaugaaguuc     4080

auuucagaau caccaacagg auauuaccgu aaaauaauau ucgaaguucc aaaugggauc     4140

uga                                                                   4143


<210>  5522
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5522
augaaaagaa uacuaggcuu agaccuaggu acuaccagua ucgguugggc acuugucaau       60

gaagcugaaa acaauaauga ggcgaguagu auugucaggc uagguguacg cguaaacccc      120

uuaaccgucg augagaaaag caacuucgaa aaagguaaag cuauuacaac aaaugcagau      180

cgucaauuaa gacacggagc uagaaucaac cuacaaagau auaaauugag gagacagaac      240

cuucaugauu gucuacaaaa acaaggaugg uuagguacgg aagcaaugua cgaagaagga      300

aaagcaagua cguuugaaac auauaaacua cgugccaaag cggccgaaga agagauuagc      360

uuacaugagu ucgcacgagu auuauuuaug uuaaauaaga aaagaggcua caaaaguaau      420

agaaaagcua auaauaaaga agacggacaa cuauucgaug ggaugacuau agcaaagaaa      480

cuuuacgagg aacaccuaac ccccgcagaa uacucuuuac aauuacuaaa caaaggaaag      540

aaauucacuc aggguuauua ccguagcgau uuaaaugccg aacucgaacg cauuugggau      600

gaacagaaga aauauuaccc cgaaauacua acagacgagu ucaaacaaca auuagaagga      660

aaaacaaaaa cgaacaccag uaaaaucuuc uuagcaaaau auggaauaua uagcgcugau      720

uuaaaaggac uggauagaaa auuccaacca uuaaaaugga gaguagaagc acuacagcaa      780

caaguagaca aggaagugcu cgcuuuugug aucagugacc uaaaaggaca aauagcuaau      840

acgucaggcu uacuuggagc uauaucagac agaaguaaag aacuauacuu caacaagcaa      900

acugugggac aauaucuuug ggcaucacua gaagaaaacc cgcauauuuc aaucaaaaac      960

aaaccguucu acagacaaga cuacuuagac gaauuugaga aaauauggga aacacaagca     1020

gcauuucaua aacaacuaac gccggaacuu aaacaagaaa uaagagacau aauaauauuu     1080

uaccaacgac cguugaaaag caaaaagagu cuaauuucag uaugcgaacu agaacaaaga     1140

aaaguaaagg cuaccauaga cggcaaagaa aaagagauaa cuauaggacc uaaaguagcg     1200

ccuaaaucau ccccaguuuu ccaagaguuu cgaauauggc aaaauuuaaa caacguccua     1260

uuaaucgaua augauacaaa ugagaaaaga ccacuagacg aagucgaaag aaaucuacua     1320

uacaaagaac uuucaauaaa agccaaacuc aguaaaacug aagcacuaaa aauacuaaac     1380

aaaaagggca agcaauggga uuuaaacuac agagaacucg aaggcaauag aacacaagca     1440

auucuuuucg acugcuacaa cagaaucauu acacuaacag gucacgaaga augcgacuuu     1500

aagaaaauca aagcgagcga aaucagacac uauguaagua caauuuucaa aaaccuuggc     1560

uucaguacag aaauauuaga uuucgauccg agcuuaaaga aacaugaacu agaaaaacaa     1620

ccaauguauc aacuuuggca ccuacuauau ucuuacgaau cugauaacuc uagaacggga     1680

aaugaaucuu uauuaagaaa auuagaaaca acuuuuggau uuccagaaga auacgcuaca     1740

guacugugug auguaguauu ugaagaagau uauggaaauc ugucugugaa agcaaugcga     1800

gaaauacuuc cauauuuaca agcagggaac gauuacaguc aagcaugugc auacgcagga     1860

uacaaucauu cgagacauuc acuuacaaaa gaagaacucg aucaaaaagu auacaaagaa     1920

cgccuagaac uauuaccuaa aaacucauua agaaacccag uaguagagaa aauauuaaau     1980

caaaugauaa acguaauuaa cgccauaaua gaugaauacg gaaaaccaga cgaaauuaga     2040

auugaaaugg cucgagaauu aaaaagcuca gccgcagaca gaaagaaaac cacacaugcg     2100

auuucgcaag gaaacgcuga aaaucaacgc aucagagaaa uacuagaaaa agaguucucu     2160

uugagcuaca uaaguaggaa ugauaucauc aaauauaaac uuuaugagga acuagaacca     2220

aacuacuaca aaacacuuua uagcgacacc uacauuacua aagauaaacu cuucucaaaa     2280

gacuuugaca ucgaacauau aauaccgaaa gcaagauuau uugacgacuc cuucucaaau     2340

aaaaccuuag aagcacgcaa cauaaauuua gaaaaaucua auaaaaccgc auuugauuuc     2400

auaaaagaaa aauacgguga agacggcgca gaagcauaua aaaagaaacu agacaugcug     2460

uuagaaaaug augcuaucag cagacccaaa uacaacaauu uauuaagggc agaagcugac     2520

auaccuucug auuuuaucaa ucgagaccua agaaacacac aauauaucgc caagaaagcg     2580

ugcgaaauuc uaggugagcu aguuaaaacc gucacuccaa cuacaggcaa aauaaccaau     2640

cgacuuagag aagacuggca auuagucgau guaaugaaag aacuuaacuu ugaaaaauau     2700

gaaaaacucg gccugacaga aauagucgaa gaccgagaug gaaggaaaau aaaaagaaua     2760

aaagauugga caaaaagaaa ugaccacagg caccaugcga uggacgcacu cgcaaucgca     2820

uucacaaaac caucauucau ucaauacuua aauaauuuaa augccagaag caauaaagga     2880

gauaguauau augccaucga aaacaaagaa cugcauuaug aagaaggaaa acuacgauuu     2940

aaugcaccaa ucccagucaa cgaguuccga gcagaggcca aaaggcaucu gagcgcuauu     3000

cuaguaagca uaaaagccaa aaauaaaguu augacacaaa augucaacaa aauaaaaaca     3060

aaacacggca uuauaaagaa aauccagcuu accccgcgcg gaccacugca caacgaaaca     3120

auauauggca ccaaaaugcg acccauaauc aaaaugguca aagucggagc agcacuugau     3180

gaagcaacaa uaaauaaagu aaguucaccc gcgauccgag aagcucuguu aaaaagauua     3240

aacgaauacu caggcaacgc uaaaaaggcc uuuacaggga aaaacacacu agagaaaaau     3300

ccuauauacc uaaacgcagg aagaacuaaa acaguaccca gucuaguuaa aacuguggaa     3360

ugggagagcu uccacccaac aagaaaacug auagacaaag acuuaaaugu ugauaaaguu     3420

guagauaaag gaauaagaga aauacuaaaa gccagauuag aagaauuuaa cggugaugca     3480

aagaaagcau uuaguaaccu ugaagaaaac cccauuuacc uagacgaagc uaagaaaaua     3540

gccuuaaaaa gagucucaau agaagggguc cucucagcaa uaccacuuca caccuugaaa     3600

aaucaggccg gaaaaccaau aacagguaaa gauggaaagc cuguauuagg uaauuacgug     3660

caaacaucga auaaccacca cauagcguuu uauuacgacg aagauggaaa cuuacaagac     3720

aacgccguau cauucuuuga agcugcugaa cgaaaaagcc aggguauccc gguaaucgau     3780

aaagacuaua acagagauaa aggauggaga uuucuauuua caaugaaaca aaacgaauau     3840

uucguuuucc cgaacgaagc cacgggauuu auccccagug aaguggacuu aacagacgaa     3900

gcaaacuacg ggaucauaag uccaaaucug uacagaguuc aaaagguguc acgaauagac     3960

aaaggaaccu cagccucaag agacuacugg uuuagacauc accuagaaac aaucuuaaac     4020

gacgacgcaa aauuaaagaa ccuagccuuu aaaaggauaa gaggcuuacu agaacucaaa     4080

gacauaauca aaguacggau uaacagcaca ggaaaaaucg uggccguugg agaauacgac     4140

uga                                                                   4143


<210>  5523
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5523
augaaaaaga uacucgggcu agaccuagga accacaucaa uaggaugggc ccuggucaau       60

gaagcagaaa cagaaucgga aaaaucaagc auaaucagac ugggaguaag aguaaaucca      120

cuuacuauag augaaaaagg aaauuucgaa aaaggcaaag cuauaacaac caauagcgac      180

cgucaacaaa gacacucagc caggauaaau cuacaacguu acaaacugag aagacagaac      240

uuaugugacu gccuccagau aggcggcuua uuaggauccg aaucaaugua ugaagaaggu      300

aaagaaagca cuuuugaaac cuacaaauua agagcaaaag cagcgacgga gaaaguagca      360

cuccaugaau uugcaagaau acuauuuaug cuaaauaaga aacguggaua caaaaguaac      420

agaaaagcaa auucgaaaga ggacggacaa gcguuugaug gaaugacaau ugccaagaaa      480

cuauaugaag agaauuuaac acccgcagaa uauagucucc aauuacuaaa caaagggaag      540

aaauuuucac ccuccuauua ucgaagcgau uuagaauccg aauuaaaccg uauuugggaa      600

gaacaaaaga aauauuaccc agaaauacug acagaugauu uuuaccaaca acuugaagga      660

aaaacaaaag uuaauacuac gaaaauuuuu cucgcuaaau acgguauaua cacggcagac      720

cuaaaaggac uagauaagaa aaugcagcca uuaaaaugga gaauugaagc acuuaacaaa      780

cagguugaua aagaaguccu aacauucgua guaucugacu uaaaagggca aauagaaaag      840

accagcgggc uuuuagggga aaucucagac agaucgaaag aacuauauuu caacaaacaa      900

acuguaggac aauauguaug ggcauccuua aaagagaacc cccacgugag cauuaaaaau      960

aaacccuucu acagacaaga cuaccuagac gaauuugaga aaauauggga aacacaagca     1020

gcauuucaca aagaacuaac accagaacug aagaaagaaa uacgcgacau aauaauauuc     1080

uaucaaagac cacuaaaaag uaagaaagga uuaauaagua uaugcgaauu agaacagaag     1140

aaagaaaaag uaauaauaga ggguaaagaa aaagaaauau uaauugguuc aaaagucgcc     1200

ccaaaaucgu caccaaugua ccaagaguuc agaaucuggc aaaacuuaaa uaauguacua     1260

uuaauagaua cagaaauaaa cgaaagcaga ccgcuagaug aagaagaaaa agcacuacua     1320

uacaaugaac uaucaguuag agccaaauua ucagaaacag aagcguuaaa aacaaucaau     1380

aagaaaggaa aacauuggga ucucaacuuu aaagaacuag aagguaauag aacucaggca     1440

guucuauuug aaugcuacaa uagaauaaua acucaaacug gacaugaaga auguaauuuu     1500

aagaaaauau cagcagaaga caucagacau uacguagcua caauuuucaa aaauuuagga     1560

uuuaauacug acauauuaga cuuuaauccu agcuuggaaa aacacgaauu agaaaaacaa     1620

ccaauguauc aacucuggca ccuauuauau ucauaugaau cagauaaaag uaggacgggg     1680

aacgaaaguu uacugaacaa auuagaagca aacuuuggcu ucccacaaga auaugcaucc     1740

guacucgcag augcgacuuu cgaagacgau uacggcaaua uaaguguaaa agccauaaga     1800

aaaauucuuc cacacuuaca cgacgguuac gauuauucag aagcguguga acgagccgga     1860

uacaaccaua gcguacguag ucuaacgaaa gaagaauuag cccaaaaagu auauaaagac     1920

agauuagauc uuuugccuaa aaacucacuc agaaacccag ucguugagaa aauccuaaac     1980

cagaugauaa acguaaucaa cgcuauaauu guagaauaug gaaagccaga cgaaaucaga     2040

aucgaaaugg caagagaacu aaaaagcuca gcagcaaaaa gagaagaaag aacuaaauca     2100

aucaaccaaa acgcagcgga aaacaaacga aucacagaaa uauuacaaac cgaguucggc     2160

uuguccuaua uuagccgaaa ugauauugug aaauauaaau uauaucaaga auuagaagcu     2220

aauggauuca aaacucucua cuccaacacc uauaucccca aagaaaaauu auuuucaaag     2280

gauuuugaca ucgaacacau uguaccaaaa gcaagacuau ucgacgauuc auuugguaau     2340

aaaaccauug aggcaagaga cguaaaucuu gcaaaaucua acaaaacagc uuucgacuuc     2400

gugaaagaaa aauaugguga acaagggauu gaagaauaua agaagaaacu agacacccua     2460

cuaaaagaaa augcuaucag uucaggaaaa uauaagaaau uaaugauguc cgaagcagau     2520

auaccaucag auuucauuaa uagagaccug cgaaacaccc aguacaucgc gagaaaagcg     2580

uacgaaauau uaggagagcu aguaaaaacc aucacaccaa cuacaggaaa aauaacugau     2640

cguuuaagag aagauuggca acugauugau guaaugaaag aacugaacuu cgaaaaauau     2700

gauagauuag gacugacgga agugauagaa gacagggacg gcagaaaaau caaaagaaua     2760

aaagacugga caaaaagaaa ugaucacaga caccaugcua uggacgcacu uaccauagcc     2820

uuuacaaaac cauccuacau ucaauaccua aauaaccuca augccagauc agacaaaagu     2880

gguagcaucu acgcgauaga aacuaaagaa uuacacagag aaaauaacac cuuacgauuc     2940

aaugcaccaa ugccauuaaa ugaguucaga aaggaagcua aacgacaccu aucagcaaua     3000

cucguaucca ucaaagccaa aaauaaagua augacccaaa auguaaauaa aauaaaaaca     3060

aagaacggca uaauaaagaa aguacaacua acaccuagag gagcauuaca caaugaaaca     3120

auauauggaa caaaaaugcg accuauaaua aaaaugguua aaaucggagc agcguuagau     3180

gaagcgacaa uuaauaaagu caguagccca acaauaagag aagcauuacu aaaacgucua     3240

aacgaauaca guggagacgc aaagaaagcc uuugcuggua aaaaugcgau caccaaaaau     3300

ccuauauacu uaaaugcaga acauacaaaa acagcccccg ccauggucaa aauagucgaa     3360

ugggagaugu auuauccaau aaggaaacua aucacuccag aucuaaaaau cgaaaaagua     3420

auagacaacg gaauaaaaga aauucuaaag aaacgacuaa aagaguucgg aggagacgca     3480

aagaaagcau uuagcaaucu cgacgaaugu cccauauauc uagaugaaga gaaaaauaua     3540

acacuaaaaa gggucucaau cgaaggagug cuaucugcaa uaccaguaca cacacucaaa     3600

aaccacgcag gaaaagaaau caaagacgaa gacgguaaag ccguacuagg cgacuacguc     3660

cagaccucaa auaaccacca ugucgccauc uaucaagacg caaagggcaa ucuacaagac     3720

aauguaauaa guuucuuuga agcagcagaa aggaaaucuc agaacguacc cauaguagau     3780

aaaaaccuaa accgcgacaa aggguggcac uucuuauaca caaugaaaca aaacgaauac     3840

uucgucuucc cuaaugaaga aacagguuuc guuccuagug aaaucgaccu aauggacgaa     3900

aacaacuacg aaaugauaag cccuaaccuu uucagagucc aaacaauguc aagagucgau     3960

uauggaaaga caaucauaag agaauaugug uucaggaauc accuagaaac uauauuaaau     4020

gacaaucuaa aacuaaaagg gcuaguauuc aagaaaauua aaagucugaa ggcauuggaa     4080

ggaauaguaa aagucagagu uaaugccaua ggaaaaauag uugcaguugg agaauaugau     4140

uga                                                                   4143


<210>  5524
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5524
augaaaaaga aauaugcgau aggaaucgau auagguacaa acagcguagg cuggagcguu       60

guaacagacg acuauaaagu cccaucuaag aaaaugaaag uguucggaaa uacagagaaa      120

agauacauca agaaaaaucu acuaggcacc uuacuauucg acgaaggaaa cacagcagaa      180

aaccgacgau uaaaacgaac agcaagaaga agauauacac gaaggagaaa cagaauauua      240

uacuuacagg aaaucuucgc cgaagaaauc aauaaaauag acgacagcuu cuuucaaaga      300

uuggacgauu cauuucucau aguggaagac aaacagggua gcaaacaucc aauauuuggc      360

acacuacaag aagaaaaaga auaccauaaa caauuuccua cuauauauca ucuucgaaaa      420

caacuugcag acucaucaca aaaagcagac auacgacuaa uauauuuagc auuagcucau      480

auaauuaaau auagaggaca cuuucuauuu gaaggagacu uaaaaucgga aaauaaagau      540

guucaacauu uauuuaacga cuucguagaa auguuugaua aaaccgucga aggcucauac      600

cuaucugaaa accuccccaa cguagcagac guacucguag aaaaagucag uaaaagucga      660

caccucgaaa auauacugca uuauuuucca aacgaaaaga aaaauggguu auuuggaaac      720

uuuuuagcgu uagccuuggg acuacaacca aacuucaaaa ccaauuucga auuagcagaa      780

gacgcaaaaa uucaguucuc aaaagaaaca uacgaagaag auuuggaaga auuacuaggc      840

aaaauaggag augacuacgc agauuuauuu auagcuacaa agucccucua ugauggcaua      900

cuacuggcgg gaauacuuuc aacaacaaau ucaacaacua aagcaccauu aagcucaagc      960

augguuaaca gauacgaaga acaccaaaaa gaccuagcuc uacugaaaaa cuucauacac     1020

caaaaccuaa gcgauagcua uaaagaagua uuuaacgaua aauuaaaaga cggauaugca     1080

ggauauauag aggguaaaac aacucaagag aauuuuuaca gauucauuaa gaaagcgaua     1140

gagaaaauag acgguucuaa uuauuucauc gagaaaauag aaagagaaga uuuucuaaga     1200

aaacagagaa ccuuugauaa cggaucaaua ccucaccaaa uucauuuaag agaaaugcaa     1260

gcaaucauaa gaaggcaagc aaaauucuau cccuuuuuag aagaaaauca ggacaaaauc     1320

gagaaaauuc uuacauucag aaucccauac uacguuggac cucuagcuag aggaaaauca     1380

gaauuugcau ggcucaacag gaaaucggac gagaaaauaa gaccauggaa cuucgaugaa     1440

augguugaua aagaaacauc agcggaaaac uucauaacac guaugacaaa uuacgaccaa     1500

uacuugccag accaaaaagu gcucccgaag cacagccuau uauaugaaaa auucgcagua     1560

uacaaugaac uaacaaaagu aagauaugua acagaacaag gaaaaucuuu cuucuucgac     1620

gcuaauauga agcaggaaau cuuugauaga acauucaaag uauauaggaa gguaacaaaa     1680

gaaaaauuaa uggauuuccu uucuaaagag uucgaugaau uuagaauagu agauuuacua     1740

ggacucgaca aggacaauaa aucauuuaac gcaucuuuag guacauacca cgaccuuaag     1800

aaaauaguuu cugaagacuu ucuggauaau ucagaaaaug aagauauuuu agaaaacgua     1860

guguuaacac ucacauuauu cgaagacaga gaaaugauac ggaaacgacu cgagaaauau     1920

aaagacguau uaaccgagga gcaaaggaag aaacuggaac guagacauua uacugguugg     1980

ggacgacuau cagcgaaacu aaucaacgga aucagagaua aagugacucg aaaaacaauc     2040

cuagacuauc uuauagauga cggaacauca aaucgaaauu ucaugcaacu aauuaacgau     2100

gauacccuua gcuuuguuga cgaaauacga cuugcucagg gaucgggaaa agcugaagac     2160

uaucgggcag aaguccaaaa uuuggcaggc ucaccggcca uuaagaaagg aauauuacaa     2220

ucacucaaaa uuguagauga auuaauugaa guaaugggau augacccaga acauauagua     2280

guagaaaugg cacgagaaaa ucaauucaca aaucaaggaa ggagaaauag ccaacagcgu     2340

uauaagaaaa uagaaaacgc aauuaagaau cucgauucaa accuuaauuc aaaaauucuc     2400

aaggaauauc cuacuaauaa ccaagcacuc caaaaugaua gauuauucuu auacuaccua     2460

caaaacggca aggauaugua uacugaugaa gaauuagaua uugaccaacu gucacaguau     2520

gauauugacc auauaauacc gcaagcauuu auaaaagaug auagcuuaga uaacaaagua     2580

cuaacaaaau cagcuaaaaa cagaggaaaa ucagaugacg uaccaucucu ugaaauagua     2640

uauaagaaga aaaauuucug gaaacaacua uuagacucac aauuaauauc ccagagaaaa     2700

uuugacaauc uaacaaaagc agaaagagga ggacuaacca augaagacaa agcacgcuuc     2760

auacaaagac aauuaguaga gacucgacaa auuacaaaac acguagcuag aauauuagau     2820

accagauuca auaccaaacu agacgaagca ggcaacagaa uaagagaccc aaaaguaaac     2880

auuaugacac uuaaaucauc cuugguauca caauuucgga aagacuacca auuguacaaa     2940

guucgagaaa uaaacaacua ucaccaugca cacgacgcuu aucuaaacgc cguaguagcu     3000

acagcccuau uaaagaaaua uccccaacuu gcgccggaau uuguauacgg ugacuaccca     3060

aaauauaacu cauauaaauc cagaaaauca gcaaccgaaa aaguacucuu cuauagcaac     3120

auuaugaacu ucuuuaaaag aguccuugua uacagcaaaa ccggcgaagu uaggauaaga     3180

ccggucauag aaguaaacaa agaaaccggc gagauaguau gggauaaaaa gagcgauuuu     3240

agaacuguaa gaaaaguguu aagcuaccca caaguaaaug uugugaagaa aacagaagua     3300

caaaaccacg gauuggaucg aggaaaaccc aaaggauucu auaacgccaa ucucucaccg     3360

aggccaaaag aagguagugu ggaaaaccua guacccgcaa aacaauccuu cgauacaaaa     3420

agauaugggg guuaugccgg cauaucaaau ucauacgccg ucuuaguaaa cgguauaaua     3480

gaaaaaggaa agaagaaaag uaaagccgaa guuaccgagu uccagggaau uaguauauua     3540

gccagaaaag acuucgacaa aaauccaaaa caauauuuac ucaaccuggg auacaaagau     3600

aucaaaagca ucauuaaacu gccgaaauac agcuuauucg aauuagaaaa ugguucaaga     3660

aggauguugg cuaguauuuu aucaacaaau aacaaacgag gcgaaauaca uaaagguaau     3720

gaaauguacc uaccagauaa auuuguaacu cucuuauacc augccaugcg cguaaacagg     3780

acuuuagaac cuggacauaa gaaauaugua gaagcccaca gacauguauu cgacgaauua     3840

uuaacauaua uacuagaaua caaugaaaag aucgucgggg ccaaagcaaa cggcaaacga     3900

auuguagaag cauauucagc acgaaaagac acagacaacc ucgaagaacu cuguaauuca     3960

uuuauaaacc uauuaaacuu aacugccuua ggcucagccg ucgauuucga guuccuaggu     4020

acaaaaauac cuagguaucg agacuacacu cccucuagcc uccuaaaagc aacuuuaaua     4080

caucaaagcg uaacaggguu auaugaaacu aggauagacc ucagcaaacu aggaggagau     4140

uga                                                                   4143


<210>  5525
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5525
augaaaaaga uacuggguuu ggacuuagga acgaccucua uaggaugggc uuuaguauau       60

gaaagcgaga augcggacga gaaaagcagu auaguaaggc uagguguaag aguaaaccca      120

cuuagcguag acgaaaaagg aaauuuugaa aaaggaaaau caaucacaac uaaugcggac      180

aggcaacaaa cgcauggcgc aagaauaaac cuacaacgau auaaguugag aagagaaaac      240

cuuaaagacu gucuacaaaa acaagguugg auaggaacag aagcacuaua cgaagaagga      300

aagaccucaa ccuuugaaac auauauguua cgagcaaaag ccgcugaaga agaaauaagu      360

uuacacgaac uuuguagagu acuuuucaug cucaauaaaa agcgaggaua caaaagcaau      420

cgaaaagcaa acaacaaaga agacgguaaa cuuuuugacg gaaugacagu ugcaaagaaa      480

cuuuacgaag aacaucuaac cccagcagaa uacuccuuaa aacuccuuuc aaguggaaag      540

aaauucaaac aggaauauua uagaucugac cuaaaagagg aguucgaucg cauauggaau      600

gaacaacaaa aauauuaccc agaaauauug accgacgauu ucaaacaaca acuacaaggu      660

aaaaguaaag gaaacaccuc caaaauauuc cuggggaaau augggaucua cucagccgac      720

uuaaaaggcu uggauagaaa auuacaaccg cuaaaaugga gaguagaggc acuaaaacag      780

aaaguagaua aagaaguauu agcauucgua auuagugacu uaaaugguca aauugcuagc      840

accucagguu ugcuuggggc uauaagcgac cgcucaaaag aacucuauuu uaauaaacaa      900

acaguaggcc aauaccuaug gacaucucua aaagaaucac cgcacguaag cauuaaaaac      960

aaaccauuuu aucgacaaga uuaccuagau gaguucgaga aaauuuggga aacacaagca     1020

accuaccaua aggaauuaac accugaacua aaacaagaaa uacgagacau aauaauauuc     1080

uaccagcgcc cuuuaaaauc uaagaaagga cuaauaucag uaugugaauu ggaacaaaga     1140

aaaguaaaag uaacaauaga uggaaaagaa aaagaaauau cgauaggacc caaaguagcc     1200

ccaaaaucaa gccccauguu ucaagaguuc aggauauggc aaaaucuaaa uaauauccug     1260

cucauagaua aaggaaacaa uucaaauaga ccauuauacg aagaagaaag aaaucuauua     1320

uaugaagaac uaagcguaaa agaaaaacug ucaaaggcag aagcacuaaa aguacuaaau     1380

aaaaauggca aacaauggga ucuaaacuau aaagaaaucg aaggaaacag aacccaagcg     1440

guacuauaug acugcuauaa ucguauuauc ggaaugacug gccaugaaga augcaacuuc     1500

aaaaaguuaa aagccucaga uauuagauac aacguuaaaa caaucuucaa aaacuuagga     1560

uuuucaacag auauacuuga cuuugacagu agucuacaag gucaugaacu cgaaagacaa     1620

ccauccuauc aauuauggca cuuauuauac agcuacgaau cugacaauag cagaaccgga     1680

aaugaugcac uacugcguaa acuggaaaca gcuuuuucga ucccaaaaga auaugcggca     1740

guauuauccg acguaguauu ugaagacuca uacggaaauu uaagcguuaa agcaaugaga     1800

aacauauuac cucaucuuca agcggguuug gauuacaguc aagcgugcga aaaggcagga     1860

uauaaacacu caagaagauc acuuacaaag aaugaauuag acaagaaaga guacaaagaa     1920

aaacuagaac uuuugccaaa aucaucucua cacaauccag uagucgagaa aauauuaaac     1980

caaaugauaa augucauaaa cgcaguaaua gacgaauaug gaaaaccuga cgaaauuaga     2040

auagaauuag cuagagaacu uaaaaucgga gccgcagagc gcgaaaauau gacaagaagu     2100

aucaaccagg guaacgccga aaacaaacgc aucagagaaa ucuuggaaaa agaauuugca     2160

cuaucauaua uaaguagaaa cgacauaaua aaauacaagc uauaugagga acuaaaauca     2220

aauggauuua aaacauuaua uagugauacu uacaucagca aagauaaacu uuucaguaaa     2280

gacuuugaca uagaacauau aauaccaaaa gcaagguuau ucgaugacag ucuaagcaac     2340

aaaaccaucg aggcucgcga cguaaacaua gcaaaaucga acaaaacagc auuugacuuu     2400

aucucagaaa aauacgacca acaaggaaua gaagcguaua aaaagaaacu ggacauuuua     2460

uuacaggaug gcaguaucuc caaagcaaaa uauaauagac uuuuaaugaa agaagcugau     2520

auaccaagug gguucauaga cagagauuug cgugauacuc aauacaucgc gaagaaagca     2580

uaugaaauac uauccgaucu uguccgcaau guuacaccaa caacaggggc gguaacauca     2640

cgacuaaggg aagacuggca auuagucgac guuaugaaag aauuaaacuu ugaaaaauac     2700

caaaaacuag gacugguaga aguaauagaa gacagagacg gucgcaaaau uaaaaggaua     2760

aaagacugga caaaaagaaa cgaucacaga caucacgcca uggaugccuu aaccauagca     2820

uuuacaaaac cgucauacau ccaguaccuc aacaaccuua augcaagguc agauaaaaac     2880

aguagcaucu auggaauaga gaauaaagaa cuccauagag aagacaauaa auuaagauuu     2940

aacgccccau uacccauuaa cgaauuucgc gcagaagccc gccgucaccu caaaucaauc     3000

uuaguaagcg ugaaggcgaa aaacaaagua augacacaaa acguaaacgu uaucaaaacc     3060

aaacacggug uaauaaagaa aguucagcua acaccaagag gggaacuaca uaaugccaca     3120

uucuauggua caaagaaaag accgaucaua aaaaugguua aagucggugc cgccuuagac     3180

gaaguuacaa ucaagaaagu aucaagcucc uuuauaagag aagcacuccu cgaccggcuc     3240

caugaauaca auggagaugc caaaaaggcu uucacuggaa gaaauucaau agacaaaaau     3300

ccaauauaca ucgaugagga acauaagaaa accguaccug cucucguaaa aacgauagaa     3360

ugggagaugu accacccuac ccgaaaaaug auuagcccag accuaaaaau agacaaaguu     3420

auagauaaag guauacggga gauacuucga acgcgucugg ccaaauucaa cggggacgca     3480

caaaaagcau uuucuaaucu agacgaaaac ccaauauaua aagacgaagc uaagaaaaua     3540

acauuaaaaa ggguaagccu agaaggagua aguucaguua ucccguugca caaguuaaaa     3600

gacaugggag ggaaaccugu uguaggcaaa gacgguaagc caguaugggg ugauuacgua     3660

caaacaucaa auaaccacca caucgcuuuc uacauggaug aaaacggcaa ucuacaagac     3720

gauguaguua cauucuuuaa ggcagcucga cguaaaagcc aagguguacc cgugaucgau     3780

aaaaacuaua aucacgacaa agguuggcau uuucuuuuca caaugaaaca aaaugaauau     3840

uucguuuuuc caaacgaagc cacugguuuu aucccgacug aaguagacuu aauggaugaa     3900

gcaaauuaug acaugaucag cccaaaucua uacagaguuc agaaaauguc uaaaacaaaa     3960

caaggaauaa ccgauaaacg agacuacugg uuucgacacc accuagaaac aguacuuaac     4020

aacgacuuaa aaguaagaaa uucaauauuc aaacaaauaa acucacuaaa accauuagaa     4080

ggcauaguua aaguaagaau aaaugccaua ggaaaaaucg uugccauagg agaauaugau     4140

uga                                                                   4143


<210>  5526
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5526
augaaaagaa uacuagggcu agaucuugga acaacaagca ucggaugggc gcuuguaaau       60

gaagcugaau ccaacaauga agcaucaagc aucguacgau uaggcguacg cguaaaccca      120

uuaacaguag augagaaauc aaauuucgaa aaagguaaag ccauuaccac aaacgccgau      180

agacaauuga ggcauggagc aagaauaaau cuucaaagau auaaauuaag acgccaaaac      240

uuacacgauu guuuacagaa acaaggaugg uuggguacag aagcaaugua cgaggaaggu      300

aaagcaucaa cauuugagac auacaaauug cgagcuaaag cugcggaaga agaaaucuca      360

cuacaugaau uugcuagggu acuauuuaug uuaaacaaga aaagagguua uaaaagcaac      420

agaaaagcga acaacaaaga agauggucaa cuguucgacg gaaugaccau agcuaagaaa      480

uuauacgagg aacaccuaac uccagcggaa uauuccuuac aacuuuuaaa uaaaggcaag      540

aaauucacac aaggcuacua ucguucagau cuuaacgcag agcucgaaag aauaugggac      600

gaacaaaaga aauacuaccc agagauacuc acugaugaau uuaaacaaca auuagaggga      660

aagaccaaaa cuaacaccuc aaaaauauuc cuagcuaaau auggaauaua cucagcagac      720

uugaaaggac uagaccggaa gcuacagccg cuuaaaugga gaguagaggc ccuuaaacaa      780

caaguggaca aagaaguccu cgcuuucgua auaagugauu uaaaagguca aauugcaaac      840

acaucaggcu uauuaggagc gauaucagau agaaguaaag aacuauauuu caacaaacaa      900

acaguagguc aguaucuuug ggcaucauua gaagaaaacc cucacaucuc aauuaaaaau      960

aaaccauuuu auaggcaaga cuaucuugau gaauuugaga aaauauggga aacccaagca     1020

acauuucaca aacaacucac accugaacua aaacaagaaa uacgggauau aauaaucuuu     1080

uaucaaagac cacuuaaauc aaagaaauca uuaauauccg uaugugaauu agaacagaga     1140

aaaguuaaag caaccauuga uggcaaagaa aaagaaauaa cuaucggucc aaaaguagca     1200

ccaaaaagcu ccccgguuuu ucaagaauuu cguauauggc aaaaccuaaa uaauguacua     1260

uuaauagaua acgauacaaa cgaaaaacgc ccacuagaug aagaugagcg cuaucuacuc     1320

uacaaagaac uaaguaucaa agcaaaauua ucuaaaacag aagcacuaaa aauauuaaac     1380

aagaaaggca aacaauggga ucucaauuac aaggaauugg aaggaaaccg aacccaagca     1440

auauuauuug auugcuacaa cagaauuaua acauuaacug gucacgaaga augcgacuuu     1500

aagaaaauaa aagcaagcga aauacgucau uacguaagua caauauuuaa aaaucuaggg     1560

uucaguacug aaauacucga uuucgaucca ucacucgaga aacacgaacu agaaaaacaa     1620

ccaauguacc aacuuuggca uuuauuauac aguuacgaaa gcgauaacuc cagaacagga     1680

aaugaaaguc uccuacgaaa auuagaaacc acauuuggcu ucccugaaga auacgcgaca     1740

guauugugcg acguaguauu cgaagaggac uaugguaauc uaucaguuaa agcuaugcgc     1800

aagauauuac ccuaucucca agcagguaac gauuauucac aagcaugugc guaugccgga     1860

uauaaucauu caagacauuc acugacaaag gaagaguuag aucaaaaagu auauaaagaa     1920

cgguuggaac ucuugccuaa gaacucucua agaaauccug uaguagagaa aauucucaau     1980

cagaugauca auguaauaaa cgccauaaua gaugaauacg gaaagccaga cgaaauaaga     2040

auagaaaugg caagggaacu uaaaagcuca gcagcagaua gaaagaaaac aacacacgca     2100

auuaaucaag gcaaugauga aaaccaaugc auaagagaaa uauuagaaaa agaguucgcc     2160

uuaagcuaua uaucccgaaa ugauaucauu aaauacaaac uauacgaaga acuaaaacca     2220

aacggauuua aaacacugua cucagacaca uauaucccaa aagauaaauu auuuuccaag     2280

gauuuugaua uagaacacau aauuccaaaa gcaagacuau ucgaugauuc auacagcaau     2340

aaaacucuag aagcacgaga caucaaucuu gcaaagucga auaaaacugc auucgacuuc     2400

gucaaggaaa aauacggaga acaaggugcu gaagcauaca aaaagaaauu agauaugcuc     2460

cuuggcaaua auguaauaag caaaccaaaa uacaacaaac uacucaugac agaagcggau     2520

auuccaagug auuuuaucaa uagagaucug cgaaacacgc aauauauagc ccgaaaagca     2580

ugugaaauau uaggagagcu aguuagaaca gucacuccca cgacuggaaa gaucacaaau     2640

cgauuaaggg aagacuggca auuaguagac gugaugaaag aacuaaauuu ugaaaaauac     2700

gaaaagcuag gcuugaccga aauaguugaa gaccgcgacg ggagaaaaau aaaaagaaua     2760

aaagauugga cuaaaagaaa cgaucaucgg caucaugcua uggacgcccu cgccaucgcc     2820

uucacuaaac caagcuauau acaguacuua aacaaucuca augcaagauc aaauaaagga     2880

gacaguauau acgcaauaga aaauaaagaa uuacauuaug aggaaggcaa auuaagauuu     2940

aaugcuccua uccccguaaa cguguuucga gcagaagcca aaaggcaucu aagcgccauu     3000

cuagucucaa ucaaagccaa aaacaaagua augacacaaa acguaaacaa aauaaaaaca     3060

aaacauggga uaauaaagaa aauucaacua acaccccgug gaccacuuca caacgaaaca     3120

auauauggca caaaaaugcg ccccauaaua aagaaaguaa aaguaggagc agcacuugac     3180

gaagcaacaa uaaauaaagu aucaagucca gcaauuaggg aaguacuacu aaaaagauua     3240

aaugaauacu ccggaaacgc aaagaaagca uucacaggua aaaacacacu agauaaaaac     3300

cccauauauc uuaacgcaga aagaacaaaa acagugcccg ccagcguaaa aaccguagaa     3360

ugggaaaauu accacccuau ccgcaaaccg auaacaccug auuuaaaagu agauaaaguu     3420

aucgauaaag guauaggaga aauucuaaaa gcccgauuag aagaauuuaa cggugacgcc     3480

aaaaaggcau uuagcaauuu agaagaaaau ccaauauacu uagaucaaac aaagaaaauc     3540

gcauugaaac gggugucaau ugagggaguu uuaucaacaa uuccccuaca cacauuaaag     3600

aaucaagcag guaaaccuau cauggggaaa gauggaaaac caguacuagg aaauuacgua     3660

caaacaucua acaaucacca cauugcauuu uacuaugacg aagauggaaa ccuucaagau     3720

aacgcgguau cauucuuuga agcagccgag cgaaaauccc aaggaauuuc cguuauagau     3780

aaagacuaua accgcgacaa aggcuggaga uuuuuauuca cuaugaaaca gaacgaauac     3840

uucguauucc caaaugaggc aacugguuuu auaccuuccg aagucgauuu aacugaugaa     3900

gcaaauuaug gaauaauuag uccaaauuua uaucguguac agaaaauguc gagaauugaa     3960

aaagggacgu ccguaucaag agacuacugg uuuagacacc acuuagaaac aauauugaau     4020

gacgacucua gacugaaaaa uagugcuuuc aaaagaauaa acucgcuaaa accacuagaa     4080

gggaucauaa aagucaggau aaacucaauu gguaaaauag ucgcaguagg cgaauacgac     4140

uga                                                                   4143


<210>  5527
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5527
augaagaaag gauacucaau aggauuagau auagguacaa acucaguugg auucgcagug       60

auuacagaug acuauaaagu cccaucaaag aaaaugaaag uucuaggaaa cacagauaaa      120

agauucauaa agaaaaaccu aauuggagca cuguuauuug acgaagguac aacagcagaa      180

gcaagaagac uuaaaagaac ggcucggaga agguacacaa gaaggaaaaa cagacugaga      240

uacuuacagg agauauucag ugaagaaaug agcaaagugg acuccucauu cuuucauaga      300

cuagacgauu cuuuccucau uccagaagau aaacgagagu caaaauaccc aauauuugca      360

acacuagaag aagaaaaaga auaucacaag aaauuuccga caaucuauca ucucagaaaa      420

caucuugcag auucuaaaga aaaagcggac cuccgucuua uauaccucgc auuagcucac      480

augaucaaau accguggaca uuuucuauau gaggauacau ucgacauuaa aaacaacgau      540

auacagaaaa uauucaauga auuuauauca auuuaugaua acgcauuuga aggauccucc      600

uuaucuggac aaaacgcaca aguagaagca auauucacug auaaaauauc aaaaagugcc      660

aaaagagaaa gaguacuuaa acuuuuuccc gaugaaaaau caacagggcu auucucugag      720

uucuuaaaac ucauugucgg caaccaagcu gacuucaaga aacauuuuga ccuagaagac      780

aaagcaccuc uacaauuuuc aaaagauacu uaugaugaag acuuagaaaa ccuacuugga      840

caaaucggug augacuuugc ugaccuauuc guagcagcaa agaaacuaua ugacgccauc      900

uuacuuucag gaauauuaac gguaacagac ccaucaacaa aagcuccacu aucggcauca      960

augauagaaa gauacgagaa ucaccaaaau gaucuuguag ugcucaaaca auucaucaaa     1020

aacaaccucc cagaaaauua ugucgaggua uuuucagacg auagcaaaaa uggcuacgca     1080

ggcuaugucg guguugauuc uaaaauaaga aaacgcaacg cgaaacuagc aaccgaagag     1140

gaguucuaua aauaccucaa aaacauacua aacaauguaa aaggcgcaga uuauuuucua     1200

gauaaaaucg aacgcgaaga cuuucuacgg aaacaaagaa cuuuugacaa uggcucgauu     1260

ccacaccaaa uacaccuuca agaaaugaac gcaauuuuaa gaagacaagg cgaacacuac     1320

cccuuccuga aagaaaauag agagaaaauc gagaaaauac uaaccuuuag aauaccauac     1380

uaugucgguc cucuagcacg uggaaauaga gauuuugcuu ggcuaaccag aaacuccgac     1440

caagcaauac gaccuuggaa cuuugaagaa aucgucgaua aagcaagcag cgcugaagac     1500

uucaucaaua aaaugacaaa uuaugaccua uauuuaccug aagaaaaagu acucccuaaa     1560

cauagucuac uuuaugaaaa guucgcagua uauaaugagc ugaccaaggu uaaauucauc     1620

gcagaagguc ugcgagacua ucaauuccuc gacagcggcc agaagaaaca aauaguaaac     1680

caguuauuca aagagaaaag aaaagugacu gaaaaagaua uaauacauua ucuacauaca     1740

guagauggau acgauggaau cgaacuuaaa ggcauugaaa aacaauuuaa ugccagucua     1800

ucaacauacc augaccugcu caaaauaaua aaggacaaag aguucaugga cgauccuaaa     1860

aacgaagaaa uucuugaaaa uaucguacau acauuaacca uauuugaaga ucgugaaaug     1920

aucaaacaaa gacuagcuca auaugacagc uuauuugacg aaaaaguuau aaaaucuuua     1980

acaagaagac acuauacagg augggguaaa cucucagcaa ugcuuaucaa cggaauacga     2040

gauaaacaaa ccgguaaaac cauacucgac uaucuaauug augaugcuua uuacaaucga     2100

aauuucaugc aacuaaucaa cgacgaugga cuaucauuca aagaaauaau acagaaagcc     2160

caagucguag gcaaaaccga ugaugucaaa caaguggucc aagaacuucc uggaucccca     2220

gcgaucaaga aagguauucu ccaaucaauu aaaaucguug acgaacuugu aaaaauaaug     2280

cgacauaacc ccgaaagcau aguuauugaa auggcaagag aaaaccaaac uacagcaaga     2340

ggaaagaaaa acucacaaca acgauacaaa agaauagaag acgcacugaa aaaccuagca     2400

ccaggauugg acucaaauau acuuaaagaa cauccaacug auaacguaca acuccaaaac     2460

gacagacuuu uccuauauua ucuccaaaau ggcaaagaua uguacaccgg cgaagcccua     2520

gacauaaauc aauuaucauc auaugauauu gaucacauaa uaccucaagc cuuuauaaaa     2580

gaugauucgu uugauaacag aguacuaacu ucaucaaaag auaacagagg aaaaagcgac     2640

aacguacccu cuaucgaagu uguacaaaaa cgaaaagccu ucuggcaaca guuacuagac     2700

ucaaaauuaa uaucagaacg gaaguucaau aaccucacaa aagcugaaag aggggguuua     2760

gaugaaaggg acaagguugg cuucauaaaa agacagcuag uagagacacg gcaaauaacc     2820

aaacacguag cucaaauacu agaugcacga uauaacacug aaguuaauga aaaagacaag     2880

aaaaaucgca caguuaaaau cauaacauua aaaucaaacu ugguaucaaa uuucagaaaa     2940

gaauuuagac uauacaaagu cagagaaauu aaugacuauc aucacgcaca ugacgccuac     3000

uuaaaugccg uggucgcaaa agcaauucua aagaaauacc ccaaacuaga accugaguuc     3060

gucuacggag aauaccaaaa auaugacuua aaaagauaua uaucacgcuc aaaagacccc     3120

aaagaaguag aaaaagcuac ugaaaaauac uucuuuuaua guaacuuacu aaacuuuuuc     3180

aaagaagaag ugcacuaugc ugacggcaca auaguaaaac gcgaaaauau agaauacuca     3240

aaagacacag gagaaauugc cuggaacaaa gaaaaagacu uugcuauaau uaaaaagguu     3300

cuuagucugc cccaaguaaa cauaguuaag aaacgagaag uacagacagg ugguuuuuca     3360

aaagaaucga uacuccccaa aggaaacucc gauaaauuaa ucccacgaaa aacaaaagac     3420

auacucuggg auacaacaaa guauggcgga uucgacuccc caguuauagc uuauagcaua     3480

cuccuaaucg ccgauauaga aaaaggaaaa gcaaagaaac uaaaaaccgu aaaaacccuu     3540

gucggaauaa caauaaugga aaaagccgca uuugaagaua acccaauuac uuuccuugaa     3600

aacaaaggau aucacaaugu ccggaaagaa aauauauuau gccuaccaaa auacucuuua     3660

uuugaacucg agaacggaag aagaagacua cuagcaagug ccaaagaacu acaaaaaggc     3720

aacgaaauug uuuuacccgu auacuuaacc acacuucuau accauucgaa aaacguacau     3780

aaauuagaug agcccggaca cuuagaauac auacaaaaac acagaaauga guucaaagau     3840

cuauuaaacc uuguaucaga guucucacaa aaauauguac uagccgaugc aaaccuagaa     3900

aagauaaaaa gucucuacgc agacaacgaa caagccgaua uagaaauauu agccaacucu     3960

uuuauuaacu uacuaaccuu cacugcuuua ggagcuccag cugcauucaa auucuuuggg     4020

aaagacauag acagaaaaag auauacuacu guauccgaga uccucaacgc aacuuuaaua     4080

caccaaucua uaacuggcuu auaugaaacu agaauugauc uaagcaaacu cggagaggau     4140

uga                                                                   4143


<210>  5528
<211>  4143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5528
augaaucuaa ggcauguagg gaauuauaau auuggcuuag auaugggaac agcaagugua       60

ggcugggcag caauagacga acaaggugag uuauugcacu uuaagggaaa accaacuugg      120

ggcucgcgac uauucgacuc agccaacaca gcagcaacag cuagaaugcc aagaggccaa      180

cgaagacgau acauuagacg aagguggcgc cuagaucuac uacaaaaauu cuuuagugaa      240

gaaauggcag aaaaagaccc ugaguucuuu auuagacuca ggcaaucauc ccucuggcca      300

gaagaucgcg aaggaggaca uacugcauac cacuggccuu uauucaacga cagugauuuu      360

aaugaaaaag aauauuauga aaaguucccg acgauuuucc auuuaagaca auggcucaug      420

gaaacagacg aaaaagccga uauucgacuu auuuaccucg ccuuccacaa uauuguuaaa      480

caccguggca acuuccuaca agaaaacaau gauagucuaa gcgcacaaaa cgcuaacaug      540

cgggagagcu uagaaucauu uugcauagcu cuagaagaau ggugcgauga aaaagaacuc      600

acaugcagcu gugacccuca aaaauuggaa cacuuauuua aagaaguaaa auauagaagu      660

gacauaagag acggauuugc ugcuuuauua ggccuagcau guagugguga aaaugaagua      720

gacguaaaag caauggcuaa agaaauggga aaggcaauag uaggauacaa agcugacuuc      780

ucaaaaauau uuacagaaug ugauaaagaa ggaagcucgu uccgguuauc ugaugaugaa      840

ggaccggaag ccuuuuuaga cauaugccca gacgauggaa ucgcacuauu cgaagcaaua      900

caaauggucu auuccucuuu uauauuaacc ggaauguuaa cagaaaaagg ccugagccua      960

ucgaaaauau cugcguauaa ucaguauggu caagacuuag aaaguuuaaa agcguuagua     1020

aaagaauaug uaccaaacga auauucuagu uucuuccgag gaccgacaua uagcggaagu     1080

acagaauaug auccuaguaa agcucaaggu uauacuagau auaauuuagg acccaguaaa     1140

aaggggagug gcgcuaaacc aauggcauau gaagauuuua agaaaucagu ugaacaacua     1200

uuugcaaaua caaacgccau aaaugacgaa agauacaaag agaugauggg aagauuugcu     1260

gaggaaaaau uucuacggag acuccgcacg uccgauaacg gacgaauacc auuucaauug     1320

aauuuagaag aaaugacagc aaucauaauu aaccagggua aauauuaccc auuccuccuu     1380

gagcaacaag agaaacucga gucacuuguu cguuuuagga uacccuacua cguuggacca     1440

cuaacccgca uaaaugcacg acuagauuca aaaggaaacg cuugcuuugc auggaguaaa     1500

agaaaagacg gcauggauag ugcaagcaua aaaccaugga auugggauga aguaauugac     1560

aaacacgcau cggcacaagc auucauugaa agaaugacag gaacauguac guauuuaaga     1620

ggggagccag ucuuaccaag auguucgcua uuauaugaag aauauugugu ucuaaaugag     1680

cucaauggag caagauggac ucaggauggu gauaaauuca uacgauuuga uuacaaagau     1740

cggucggaca uaaucgaaga acuuuuuaag aaaagaagag gaaggauuca auacgcagca     1800

guugccgauu ggaugaaaca agaacguuca caugcuaacg ugcacgucuc gggaggccaa     1860

ggagaaaaug cuuuugaauc uagguuaucu ucuuaccauu ucuuuuguaa cgaugucuuu     1920

ggaguggaug aacuaccagg auccuacgua gauaugguug aagaaauaau auuauggucu     1980

acauuguuug aagauagaag uauacuaagg gaagaaauac aaaggaaaua uggagacgua     2040

uuaaacgaag aacaaauuaa aacaauuugu aagaaaagau uuucaggaug gggccgccua     2100

aguagaaaau uguuauguag ucuaaaagca caaaccgaca augguccaaa aucuauaaug     2160

gauauacuac aagaaggaga ucaaaauaac ggcagaguag gcagagcaau gguauuucag     2220

gaaauacucc acgaugaaca auuagguuuu gaacagaaaa uagaagaaga aaauacacaa     2280

aguauguuag aaggagaaaa ccugcuaaua gaagauuuac cagguucacc ggcaauaaga     2340

agaucuauaa aucaggccgu caggauagua gacgaaauag uggggauugc ugggcacgca     2400

ccagauaaua uauucaucga aguuacacga gaagacgaua cuaaaaagaa aggaaaaaga     2460

acaacgacca gauauaguaa uauacaaaaa gcacuagaag cauuaaaaga agaaggcgcu     2520

gaggcuuuac gggaacuaaa gacguauaaa cacaacgaac uggacaaaag auuaucccua     2580

uacuuuaugc aaaaugggaa aucccuuuau agcggcauuc cgcuagacau cagaaggcua     2640

agugaauauc aaauagauca uaucauaccc caaucauaca uuaaagacga uucauacgaa     2700

aauauggcuu uaguacuccc uggugaaaac caaucaaaag cagauaacuu acuaaucucc     2760

caagauaucc guauuaaaau gaaggcaacc uggacagcgc uccacaaggc gggacugaug     2820

ggagacaaga aauacaauaa ucuaaugaac aguucaaucu cagaugcaaa gaugaaagga     2880

uucauuaacc gucaacucgu cgaaacuuca caaucaguaa agcuaacgca gaugauguua     2940

ucacaaaaau auccaggagc aaaaguacgg cccauaaaag cuagcauaag uucgcagcua     3000

agagacgcuu gugacuuagu aaagugcaga gaagcuaaug acuaucauca ugcacacgau     3060

gcauaucucg cuugccaggu gggacguuuu guauuaaaac gacacucuag cauguucgau     3120

aaucccauag guaugaccaa aguaauuaga gauuucguga aacgacaagg gcaagaguuc     3180

aaacggaacg ggaaauuacc agcauacgca ucuaggucag gguucauagu acaaucuuuu     3240

augcgguccg gauucgaugc agaaacaggg gaacuguuaa gagacgacug gaaugcaccu     3300

uuugaaauag caagaaucaa aagaugucua aacuauagag acugcuauau uucacgaaug     3360

ccaauggaag auucaggagc auucugggac gcaacaauau acucuccuaa ggcaggcaag     3420

aaaggggccu uaccaguuaa acaaggauua ccaguugaaa aguauggaag cuacagcaga     3480

gaacaauuug cauacuucuu uguauacaag gcauacaauc cgaaaaagaa agcauacaga     3540

cuugaauuug aagcagugcc aguaagagua gcaagugccg uggaaacaag agaaaaugca     3600

cuagaagaau augcuaaauc aguggcaaag caagcagguc uggaguucgu agaaguagca     3660

cgaccaaaag uuuauaaaua ucaauuaauu gaauuaggcg aagauagauu guacauuacu     3720

ggaaaacgag aaguccgaaa cgcaagucaa cuugcauuca gucuagacga aauagauuua     3780

cuaaaaaggc ucgcugaagg agaagaaacu agcuccgaag aaagagaacg auuguuugau     3840

guauuaauug aaaaauacgc aagauacgcc ccaagacuag cacgacaacu aucaguagau     3900

agcauguccg cuacauucga aacccuuaac gcagaagaac aauugggaau cuuauuauca     3960

uuaguggaga uugcuaaugg aaaaagaaau augauagauu uaagcagcgc aggaggaucc     4020

aaaaacgcag gcugcaugaa cccaacuuuc aguucagaac uaaguucacc aggaaguaau     4080

uuuacuuuca ucgaccaauc aguaacagga auguucgaac gccgucaaag acuagaacug     4140

uga                                                                   4143


<210>  5529
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5529
auggucaaaa agaaauacgc aauaggaaua gacauaggca caaacucagu aggaugguca       60

auaguaacag augauuacaa aguaccuagu aagaaaauga aaguuuucgg aaauacagag      120

aaaagauaca uuaagaaaaa ccuacuagga acacuucucu uugaugaagg caacaccgcu      180

gaaaacagaa gacuaaaacg aacugccaga cguagauaua caagaagacg caauaggaua      240

uuauaucugc aagaaauauu ugcggaagaa aucaauaaaa uugacgauag cuucuuucaa      300

cgacuugacg acagcuuuuu aauaguagaa gauaaacaag gcucaaaaca cccaauauuu      360

ggcacacuuc aagaagaaaa agaguaccac aaacaauucc caacaauuua ucaccuaagg      420

aaacaacuag cugacucuuc acaaaaagcu gauauaagac uuauauaccu ggcauuagca      480

cacaucauaa aauauagagg gcacuuuuua uucgaaggag aucucaaauc cgaaaauaaa      540

gacguucaac acuuauuuaa cgauuucguu gaaauguucg auaaaacagu ugaaggaucu      600

uauuuaagcg aaaacuuacc aaacguagcc gauguucuag uagaaaaagu uagcaaaagc      660

aggagacuag aaaacauccu acacuauuuc ccaaacgaga agaaaaaugg uuuauucggu      720

aacuuucuag cucuugcgcu aggauuacaa ccaaauuuua aaacaaacuu ugaacuagcu      780

gaagacgcca aaauucaauu uaguaaagaa acauacgaag aagaccuuga agaauuauua      840

ggcaaaauag gugaugauua ugcagauuua uucauagcaa caaaaucauu auaugacggc      900

auuuuacugg cagggauacu aagcacaacc gauaguacaa ccaaagcacc acucagcuca      960

ucaaugguga auagauacga agaacaccag aaagaucuag cccuucuuaa aaauuucaua     1020

caucaaaauc uaucagacag uuacaaagaa guuuuuaacg auaaauuaaa agacggauau     1080

gcugguuaua uagaaggcaa aacuacccaa gaaaauuucu auagauuuau caagaaagca     1140

auagagaaaa uagaaggaag uaauuacuuc auugacaaaa ucgacagaga agacuuuuua     1200

cgaaagcaaa gaacuuucga uaauggcucc auaccucauc aaauacaucu acaagaaaug     1260

cacgccauaa uacgacgaca ggcggaauuu uauccguuuu ugguagaaaa ucaagacaag     1320

auagagaaaa uauuaacauu ucgaaucccu uacuaugucg gaccacuagc uagaggaaaa     1380

agcgaauuug cuuggcuaaa cagaaaauca gaugagaaaa uucgaccaug gaauuuugac     1440

gaaaugguag acaaagaaac aagcgcagaa aacuucauca ccagaaugac aaacuaugau     1500

agguauuuac cugaccaaaa agugcuucca aaacacaguc uuuuauauga aaaauucgca     1560

guauauaacg aauuaacaaa aguaaaauuu auagcggaag gaaugcgcga cuaccaauuc     1620

uuggauucag gacaaaagaa agacauugua aaaaccuuau ucaaaacaaa aagaaaaguc     1680

acagcuaaag auaucaaagc auaccuagaa aauaguaacg gguacgccgg uguggaauua     1740

aaaggauuag aagaacaauu uaacgcgagu cuaccaaccu aucacgauuu auuaaaaauc     1800

uugagagaca aagcauucau agacgcagaa gaaaaccaag aaauacucga agacauagua     1860

cuaacacuaa cacuauuuga agaccgagaa augauaagaa aaagguuaga aaaauacaaa     1920

gauaucuuaa cagaagaaca acgcaagaaa cuagaaagga gacacuauac cggauggggc     1980

agacuaagcg ccaaauuaau aaacggcauu cuagacaaag ugacucgaaa aacuauauua     2040

ggauacuuga uagaugaugg aaccuccaau agaaauuuca ugcaacuuau uaacgaugau     2100

acauuaucau ucguugauga aauaagauua gcacaaggau caggagaagc agaagacuac     2160

cgugcugagg uucaaaacuu agcaggcucc ccagcaauaa agaaaggcau acuacaauca     2220

cuaaaaauag uugaugaguu aaucgaaguc augggauaug aacccgaaca cauaguggua     2280

gaaauggcaa gagaaaauca auuuacaaau caaggacgac gaaauaguca acaaagaauu     2340

aauagauuag agaacgcacu caaagacuuu ggguccaaaa uacuagaaga acaaaaaccu     2400

uacggaguaa gcaaaguaaa uaauauacau cuucaaucag auagacucuu ucuauacuac     2460

uuacaaaacg gaaaagauau guauacugac gaagaacuag acauagauca ccucucugaa     2520

uaugacaucg accacauuau cccacaggcu uucauuaaag acgauucacu cgauaauaaa     2580

guauuaacua aguccgcgaa gaacaggggu aaaucggacg auguacccag uauggaaauc     2640

guacauaacc ggaaaaauuu cuggagacaa cuccuaaaau cuaaauugau aagccaaaga     2700

aaauuugaua acuuaaccaa agcugaaagg ggaggccuaa cuaacgagga caaagcuaga     2760

uucauccaac gucaauuagu ugaaacucga caaauuacga agcauguagc aagaauguug     2820

gacacaagau ucaauacaaa auuagacgaa gcagguaaca gaauccgcga cccaaaagua     2880

aauaucauaa cccuaaaguc uaaccuugua agccaauuuc gaaaagauua ucaacuauac     2940

aaaauccgag aaaucaacaa cuaucaccau gcccacgacg cauauauaaa cgcaguagua     3000

gcuacagccu uacuaaagaa auauccccaa cuagcuccgg aguucguaua cggggaauac     3060

ccaacauaca acucauacaa uucaagaaaa agugcaacug aaaaaguucu cuucuacuca     3120

aauauuauga acuuuuucaa aagaauagug guacauucaa aaaccggaga aguucguauu     3180

cgaccaguaa ucgaagugaa caaagaaacc ggcgaaauag uaugggacaa aaagucagau     3240

uuccgcacug uaagaaaagu ucuuagcuau ccacaaguaa augucgucaa gaaaacugaa     3300

guccagaauc acggauuaga ccguggaaaa ccaaaagguu ucuacaacgc gaaccuaagc     3360

ccaagaccga aagaaggauc aguagaaaau cuagucccag ccaaacagag uuucgauaca     3420

aaacgauaug guggguaugc aggcauauca aacagcuacg cagucuuagu gaacgguauu     3480

aucgaaaaag gcaagaagaa aucaaaagcg gaaguaaccg aguuccaggg aauuucuauc     3540

cuggcccgca aagacuucga uaaaaaccca aaacaauacc uacucaacuu agguuauaaa     3600

gauauaaaaa guauaauaaa acucccaaaa uacucguuau ucgaauuaga aaauggaagc     3660

cgaagaaugu uggcaaguau acuuaguaca aacaauaaaa gaggagaaau ucauaagggu     3720

aacgaaaugu aucugccuga uaaauucgua accuugcuau aucacgccau gagaguuaac     3780

aggacguuag aaccgggcca uaagaaauau guggaaacac acagacaugu uuucgacgaa     3840

cuacugaucu auaucuugga auauaacgag aaaaucguag gagcaaaagc aaacgggaaa     3900

agaaucgugg aagcauauuc ggcacgcaaa gacacagaca aucucgaaga acucugcaau     3960

aguuuuauaa aucuauuaaa ucuaaccgca uuaggaagcg ccgucgauuu ugaauuuuua     4020

ggcacaaaaa uaccccgaua cagagauuau acaccgagua gccuauuaaa agcaacacuu     4080

auccaucaau caguaacagg acuauaugaa acaagaauag aucugagcaa auuggguggc     4140

gacuga                                                                4146


<210>  5530
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5530
augaauuaug uaaaggaaua ucauauagga cuagauauag gaaccucauc aauaggaugg       60

gcaguuaccg auucacaauu caaauugaug agaauuaaag gaaaaaccgc aauuggcgua      120

cgacuauuug aagaaggaaa gacagcagca gaaagaagga cauuuagaac aacaagacgc      180

aggcucaaaa gaagaaaaug gagacuccau uauuuagaug aaaucuucgc accccaucug      240

caagagguag augaaaauuu cuuacguaga uuaaaacaau cuaauauaca uccagaagau      300

ccagcaaaga aucaagccuu uaucggaaaa cuuuuauuuc cagaccuuuu aaagaaaaau      360

gaacguggcu auccgacucu gauuaaaaug cgagaugaac uaccagucga acaacgagca      420

cauuacccag ugacaaacau auauaaacuu agggaagcga ugauaaauga agauagacaa      480

uuugaucuaa gggaaguaua cuuagcuguu caccauauag uuaaauauag aggucauuuc      540

uuaaacaaug cuagcguaga uaaguuuaaa guagggagga uagauuuuga uaagucuuuu      600

aauguccuca augaagcgua ugaagaacua caaaacggcg agggaucauu cacaaucgaa      660

ccgaguaaag uagaaaagau aggccaauua uuacuagaca cuaaaaugag aaaacucgac      720

cgacaaaagg caguagcaaa auuccuggag gugaaaguag cugauaaaga ggaaacaaaa      780

agaaacaaac aaauugccac agcaauaucc aaacuugucu uagguuauaa ggcugauuuc      840

gcaacaguag cuauggcuaa ugguaaugaa uggaaaauag acuugagcuc agaaacauca      900

gaggaugaaa ucgaaaaguu cagagaagaa uugagugaug cccaaaacga uauccuaacu      960

gaaauaacau cauuauucag ccaaaucaug cuaaacgaaa ucguccccaa cgguauguca     1020

auaucagagu caaugaugga uagauauugg acacaugaac ggcaauuagc ggagguaaaa     1080

gaauaccucg caacccaacc agccuccgcc cgaaaagagu ucgaucaagu cuauaauaag     1140

uauauaggcc aagcgcccaa agaaaaaggc uuugaucuag aaaagggacu uaagaaaauc     1200

cucagcaaga aagaaaacug gaaagaaauu gaugaauuau uaaaagcggg ggauuucuug     1260

ccaaaacaaa gaaccuccgc caauguagua auaccccacc aaaugcacca acaagaacuu     1320

gaucgcauaa uagaaaaaca agcaaaauac uauccguggu uagcuacgga aaacccagca     1380

acaggagaaa gagaucguca ucaggccaaa uaugaauuag aucagcuagu caguuucaga     1440

auaccguauu auguuggacc ccuaguaaca ccagaagucc aaaaagcuac uucaggagca     1500

aaauuugcau gggcuaaacg aaaagaagau ggagaaauua caccauggaa ucuaugggau     1560

aaaaucgauc gagccgaauc ugcagaggcu uuuauuaaaa gaaugacagu aaaagauaca     1620

uaucuccuua augaagaugu gcuuccugcu aacucgcuuc uauaucaaaa guacaaugua     1680

uugaacgaac uuaacaaugu aagagugaau ggaagaagac uaucaguugg aauaaagcaa     1740

gauauuuacu cagaacucuu caaaaagaag aaaacaguaa aagcaaguga uguagccagc     1800

uuagucaugg caaaaacgcg aggcguaaac aaaccaucug uugaaggucu cucugaccca     1860

aagaaauuua acaguaaucu cgcaacauau cuugaucuua aaucaauagu aggagauaaa     1920

gucgaugaua auuguuacca aacagaccuc gaaaauauaa uugaauggcg uucaguguuc     1980

gaagauggag aaauauuugc cgacaaacua acagaaguag aauggcucac agaugaacag     2040

agaucagcuc uaguuaagaa aagauacaaa gguuggggaa gacuaaguaa gaaauuauua     2100

accggcaucg ucgaugaaaa cggucagaga auaauagacc uaauguggaa cacagaucaa     2160

aacuucaugc aaauuguaaa ucaaccagua uucaaagaac agauugauca acucaaccaa     2220

aacgcuauua ccaaugacgg aaugacacuc agagaacgag ucgaaucugu auuagaugau     2280

gcauacacaa guccacaaaa caagaaagca aucuggcaag uagucagagu uguugaagac     2340

auaguaaaag ccgucggaaa ugcaccgaaa ucaauaucga uagaguuugc cagaaaugaa     2400

ggaaauaaag gagaaaucac acgcucuagg cggacucaac uacaaaaauu auuugaagau     2460

caagcacaug aauuaguaaa agauacaagu cucacagaag aacuagaaaa agcguuggac     2520

uugucugauc gauauuauuu auauuucacu caaggcggaa aagauaugua uacaggugac     2580

ccgauaaauu uugacgaaau aaguacaaaa uaugacauag aucauauauu accacaaucu     2640

uuuguaaaag acgauagccu agauaauaga guauuaguuu cgagagcaga aaauaauaag     2700

aaaagcgaua gaguacccgc gaaacucuau gccgcaaaaa ugaaaccaua uuggaaucaa     2760

cuacuaaaac aaggauuaau cacacaacga aaauuugaaa auuuaacuau ggauguagau     2820

caaaauauca aauauagauc auuaggguuu gucaaaagac aguuaguuga aacucgacaa     2880

gucaucaaac ucacugcgaa uauuuuaggu ucuauguauc aagaggcagg cacagauauu     2940

auagaaacca gagcaggacu uacgaaacaa cugcgagaag aguucgaccu uccaaaaguc     3000

cgugaaguaa acgacuacca ccaugcuguu gacgcauauu uaacaaccuu cgcaggacaa     3060

uaucuuaaua gaagauaccc uaaacuaaga aguuucuucg uguaugguga auacaugaaa     3120

uuuaaaaggg guucagauuu aaaauuaaga aauuuuaauu ucuuucauga acuuauggaa     3180

ggagauaaau cacaaggaaa aguaguagau caacaaacag gagaacuuau aacgacgaga     3240

gaugaaguag ccaaaucuuu cgauagacuu uuaaauauga aauauauguu agucagcaaa     3300

gaaguacacg auagguccga ucaauuauau ggagcaacaa ucgucacagc aaaagaauca     3360

ggaaaguuaa caaguccgau agaaaucaag aaaaaccgac cagucgaccu uuacggugca     3420

uauacuaacg gaacuucggc auucaugaca auaauuaaau ucacugguaa caaacccaaa     3480

uauaaaguua uagguguacc aacaacauca gcagucggau uaaaaagagu aggaaaaccg     3540

ggcucagaau cauacaacca agaauuacac agaauuauaa aaucaaaucc uaaggucaag     3600

aaagauuucg aaauaguggu accgcaugug ucuuacucac aacuaauagu agacggugau     3660

uguaaauuua cauuagccuc cgauacauac caacacccag cgacacaacu ugugcucagu     3720

aaagaaagua uggaaaucau cgcugacgau uucaaauuug uaaaagaaaa uccggcaaca     3780

gccgacgaac agcuagucag aguuuuugau gaaaucguca accaaaugaa ucacuauuuu     3840

acaauauuug accaaagaag caaccgacaa aaaguuacaa aagcccguga caaguucgua     3900

uccuuaccaa cagagagcga auacgaagga gcaaagaaaa cgcaagucgg aaaaacugag     3960

guaauuacca accuacuaau gggccuccau gcaaaugccg cacaaggaga ucuaaaagua     4020

cuuggccuau cuaccuuugg uuucuuucag ucaagcggag gucucaacuu aagugaggau     4080

gcaaugauag ucuaccagag ccccacagga uuauuugaaa gaagaaucug uuuaaaagau     4140

auauga                                                                4146


<210>  5531
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5531
augaaacaga uccucaagaa aguucaagga uauaacauag gccuagauau cgguacaaac       60

ucaguaggau ggguaguaac ugacgaaaau gccaaucuac ugcaauuccg uaaaaaaaac      120

auguggggug caaggcuguu ugaaaaaggc gacacagcac aagguagaag acuuucaaga      180

ucaaccagaa gaagauauga uagaagaagg cagcgaauac acuaucuaca agagauguug      240

gcaaccaugg uacuaccaau agacgaaagu uucuuuauaa gauuaaauga agcauuucua      300

uggaaagaag auaaaagugu acaaagagac uacauucugu uugcagacaa gcaauucacc      360

gacagagauu ucuacaaaca auuuaaaaca aucuaccacc uacgaaaauu ucuucuagac      420

acuaaagaaa aagcugaccc acgauuuaua uacuuggcgc uacaucacau gauaaaguac      480

agagguaacu uuuuauacca gggacaauca uuugacgucg uagagaacgu acaagaauca      540

uucagaguau ugcuagaaga aauguacgag caauuaggca aagaauggga auauggagag      600

cacuucgacg acauccaacg gauacuagaa aauacaacaa uaggcaaaaa aucuaaacaa      660

gaacaaaucg ccgccauacu caccgaacag uauggcaaca aaaaauuggc aaaugaagua      720

acaagagcau gucuagguua cucauucgac cuaacaguug ugauggaaaa ugaaaacuua      780

cuagaugaau cagguaaaaa gaucaagauu ucauucagcg acaguaagua ugaagaacaa      840

gaauuagaac uccaaaauag acuggaagaa aaauuugccg ucauagaggc ccucaaacga      900

auauauaacu ggcuuguucu caaagacacc uuacgaggaa aaaaguauau aagcgaagca      960

augguaaaca aauacgaagc acaccacgaa gaucuaguga aacuuaaacg ccuauuuaga     1020

acuuaccuau caacagaaga auacucgugu uucuuucgaa aagaaaagca aaaagauaaa     1080

uauauaucaa auuacgucaa cuauagaaaa ggcgcagaga gauguuccaa agaaaaauua     1140

uauaaagcga uacaugaacu auucgacacg guugcaaaag augacguugu cuaucaagag     1200

auaguugaaa aaauggauca aggcgaauuc cuuaccaaac aaaaugaagu caguaacgca     1260

uacauccccu accaauguaa ugaaguagaa augaaacgaa uauuaaaaca acaaggagaa     1320

uucuacccgg aacuaaaaga gaacgcagau aaaauauuac aacuguuguu auucaggaua     1380

cccuauuaug uagggccccu aguugacgaa aagcaaucga aauucgcaug gaugaaaaga     1440

agaacuggca uggaacaagu uaagauauac ccauggaauu ucgaacaagu aguagacaua     1500

gaccagacug cagaagccuu cauuacacga augacaaacu acugcacuua ucucccagaa     1560

gagaagguuc uacccaaaca aucauuacuu uacagugaau acgaauuauu acaagagcua     1620

aacaaaauaa agauaaacgg uaaguucucg uuagaaaccc gaaacaaaau aauagaacaa     1680

uuguuuaaaa aacaaaagaa aguaaccgaa aaacaauuaa agaaauggcu aguacgagag     1740

caauacugua caacgaaagu aacggaaaaa augguaaaac uagaugaaaa ugcuuauuau     1800

aucacuggau uucagaagga aaaugaauuc gcaaguagcc uaaccuccua cauagauuuc     1860

acuaaaauau uaggggaaau aacggaaaga aacagaaaaa ugauagaaga aauaauauau     1920

ugguugacag ucuucgagga aaaaucaaua gugaaaagaa aaauacaaaa aaaauacgga     1980

aacgaaauaa augacaaaca auuagcacaa auccuaaaac uaaauuacuc uggauggggc     2040

cgauuuagug aaaaauuuuu aaauggaauc aaaggauucg accgacaagg acaaaaaaaa     2100

acaaucaugg agcucuuaag ggaagacuca cgacuacuaa acauuaugca aauaauaaac     2160

aaugaagaaa ugggauuuug caaaauaauc gaagcaaacc aaaaaaaacu auacggaaac     2220

gaaaaucuau uagauguagu acaagaauua cacggaagcc ccgcaauuaa aagagguaua     2280

uggcaaagcc uuaaaauagu ugaagagaua auaaguauaa ugggaggcgu cccgcaaaau     2340

auauucauag aauucgcuag agaagaugac gaucgaguaa gaaccaauuc ucguguaaaa     2400

caaauagaaa gacuauauaa aaaauuuaaa gaggagguua acgacuucaa ugagcacgcu     2460

gccaacgaac uaaaggagaa acgauaccaa aaccguauug augaagaaaa aguauuccuu     2520

uacuucaugc aaaacgggaa aagccuauac ucaggaguaa cccuggacau ugaucgauua     2580

aacgaauaug aaguagauca cauaauacca agaacgcuag uuaaggacga uagcuuagaa     2640

aacaaagcac uaguauugaa aggagaaaau cagagaaaag gagagggacu gauaccgcau     2700

gaauggcaaa aucgacagaa agaaguaugg aaaaaauuau uaagcugcgg gcugaugggg     2760

aaaaagaaau acgcaaaucu auguaaagaa ucuuuuggaa augagaaaga acaaaaacau     2820

uucauagcgc gacaaauugu ugaaacaagg caaauaauaa aacaaguagc aaacauacuu     2880

gcgcaauauu aucaaggcgu aaacguugca gcaguaaagg cuucacucgg aagugaauug     2940

cgugagaacu acacaauuga aaaaaaagac gacaacgguu uuuucaugga aaauaagagu     3000

uacaccaugu uaaaaucaag aaaaauaaau gacuuccacc acgcucagga cgcauaccug     3060

gcaugugcag uaggacuaua uauaaaagcc gcuuuuccua aauaugaaaa agaaauugau     3120

uacgaagguu aucgaaagua cuaccagaaa aaaaaccagc aaaaagaugg aguuagaaac     3180

gaaaaaagac aauuuuuuag uuauguaaug ucuagauucu cugaacaaau aguagacaaa     3240

gaaacaggag aaauauuaug ggacggaacc caaaaauuag cuuacauuaa gaagauauuc     3300

ggauacagag acugccugau cacuaaaaaa uuagaagaaa auacaggcga auucuauaag     3360

caaacaauaa augcgaagcc agaaaaagga aaagaaaaca acacaaaacu ccucccaaua     3420

aaaaaaggac ugagcguaga aaaguaugga ggauaucaag guggugaagc cgcauacagc     3480

ugccuaguuc gauaucaaca aggucgaaaa acaaaacuau cacucgaaaa aauaucagua     3540

auaauagcaa aaaaaauugc agaaaaagaa gucucacuag accgauacuu aaaggaaaaa     3600

cuagggguag aagaaauagu uauacuccgg aaaaagguua ugaaaaauca acugauagaa     3660

gauagugaug gaaacagguu cuacuuaacc gcagcuacag aaguggugaa cgccaaacaa     3720

uuuauuuugg acggaaagca ccagcaccua cuaagaacaa uagcaauaau ggagaaagga     3780

gaauauuaug gguacagacu agaugaacua gaaaauagua uggaugaguu auaugcauau     3840

uaucggauga aaauacaaca gcaauaucca gcauacgaau cgauagugaa aaaaauagua     3900

gaaagccacg uguacgaaag ccuucaugua gaagaaaaaa uaaaggccgu acaggaaaaa     3960

gguaaauucc uaauucaacu auugagacuu acggaagcua augccgaaug uauagaucuc     4020

aaaacaaucu ccgacgggcg gcucgcagac cguauaggga gaaaaaauca cuacacaauu     4080

aaagaaggca uaaaauuuau agaucaauca auaacggguc uauaugaaag auauaugacg     4140

guguga                                                                4146


<210>  5532
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5532
auggacuacg uaaaagaaua ucacaucgga cucgauaucg gaaccucaag uauuggcugg       60

gcuguaacag auagucaauu uaaacuaaug aggauaaaag guaagacagc cauagggguc      120

agacuuuucg aagaaggcaa aacagcagca gaaaggagaa cuuuccgaac aacaagaaga      180

cgacuaaaac gccguaaaug gcgacuacau uaccuagaua agauauucgc accacaucua      240

caggaagucg acgaaaacuu ucuccggcgc uuaaaacaga gcaacauuca cccagaagac      300

ccagccaaaa accaagcauu cauuggaaaa cuacuauucc ccgacuuguu aaagaaaaac      360

gaaagaggau auccuacauu aauaaaaaug agagaugaac uuccaguaga gcaacgagcu      420

cauuauccag ugacaaacau auacaaacua agagaagcaa ugauuaacga ggaucgacaa      480

uucgauuuaa gagaagucua ucuugcagua caucacaucg uuaaauacag aggacacuuu      540

uuaaacaacg ccagcguaga uaaauuuaag gucgggagaa uagacuuuga uaagagcuuu      600

aacguacuaa augaagccua cgaagaauug caaaacggag agggaucauu cacuauagaa      660

ccaucaaaag uagagaaaau agggcagcua uuauuagaca ccaaaaugag aaaauuagac      720

aggcaaaagg cagucgcaaa auuauuagaa guaaaaguag ccgacaaaga agaaacuaaa      780

agaaauaaac agauagcuac agcaaugucc aaauuaguac uagguuauaa agcggauuuc      840

gcaacaguag ccauggcuaa cggaaacgaa uggaaaauug aucuauccag ugaaacaagu      900

gaagaugaaa uagaaaaauu uagagaagaa cuaagugacg cacaaaauga uauucuaacc      960

gaaauaacua gcuuauucag ccagauaaug uuaaacgcaa uaguaccaaa cggaauguca     1020

aucuccgaau caaugaugga cagauauugg acccaugaaa gacaauuagc agaaguaaaa     1080

gaguauuuag cgacacaacc cgcuucugca agaaaggagu ucgaucaagu auacaauaaa     1140

uauauaggac aaguaccaaa agaaaaaggu uuugacuuag aaaaaggacu gaagaaaaua     1200

cuaucaaaga aagaaaauug gaaagaaaua gacgaacuac uaaaagcugg ugacuuucua     1260

ccaaaacagc gaacaucugc aaauggagua auaccacauc aaaugcauca acaagaacua     1320

gauagaauaa ucgaaaaaca agcaaaauac uauccauggu uagcaacaga aaacccagca     1380

accggagaac gagaucgaca ucaagccaaa uacgaauuag aucagcuagu uaguuuuagg     1440

aucccuuacu acguaggucc acugguaacc ccagaaguac aaaaagcaac caguggugcg     1500

aaguucgccu gggcuaaaag aaaagaagac ggggaaauua caccauggaa uuuaugggau     1560

aaaauugaca gagcagaauc ugcagaggcc uucauaaaga gaaugacagu aaaagacacu     1620

uacuugcuaa augaagaugu ccuccccgca aacucacuac uauaccaaaa auauaaugua     1680

uuaaaugaac uaaauaacgu aagagucaau ggucgcagac uguccgucgg aauaaaacaa     1740

gauauauaca cggagcuguu uaaaaagaag aaaacaguca aagcgggaga uguugcaagu     1800

cuaguaaugg caaaaacaag aggaguaaau aaaccaucag uagaaggucu uucagaccca     1860

aagaaauuca auucaaaucu agccacauac uuagauuuaa aauccauuau gggagauaaa     1920

guagacgaca auagauauca aauggacuua gaaaauauua uagaauggcg aaguguauuu     1980

gaagacggag aaaucuuugc agacaaacuu accgaaguag aaugguuaac agaugagcaa     2040

agaucggcuc uaguaaagaa aagauauaaa ggguggggaa gauugucgaa gaaacuacuu     2100

accgguaucg uagaugaaaa uggacaaaga aucauagauu uaauguggaa cacugaucaa     2160

aacuuuaugc aaauaguaaa ucaaccagua uuuaaagaac aaaucgauca acuaaaccag     2220

aaagcgauca cuaaugacgg aaugacccuu cgugaacgug uagaauccgu ucuagaugac     2280

gcauauacau caccgcaaaa uaagaaagca auuuggcaag ugguccgugu aguagaagac     2340

auuguaaaag cuguugggaa ugcaccaaaa ucgauaucca uugaguucgc cagaaaugaa     2400

ggaaauaaag gagaaaucac aagaagcagg aggacucagc uucaaaaacu cuuugaagac     2460

caagcucacg aauuagugaa agacacuucu uugaccgaag aauuagaaaa agcuccagac     2520

uuaucugaua gauacuacuu uuacuuuaca caagggggaa aagauaugua uacaggggac     2580

ccaauuaauu ucgacgaaau aucuacuaaa uaugauauug accacauacu accgcaguca     2640

uuugugaaag augauucauu agauaauaga guacugguuu cuagagccga aaacaauaag     2700

aaaucugaca gaguaccagc aaaacuauac gcugcaaaaa ugaaaccaua cuggaaucaa     2760

cuacuuaaac agggacuaau uacucaaagg aaauucgaaa acuuaacuau ggacguagau     2820

cagaacauua aauacagauc uuuaggauuc guaaaaaggc agcuagucga aacacgacaa     2880

guaauaaaau uaaccgccaa uauauuaggc aguauguacc aagaagccgg aacagauaua     2940

auugaaacua gagccggauu gacuaaacaa cuacgugaag aauuugacuu accaaaaguc     3000

cgugaaguaa acgauuauca ccaugcaguu gacgccuauc ucacuacguu cgcagggcaa     3060

uaccuuaaca ggagguaccc uaaacuuaga aguuucuuug ucuauggaga auauaugaaa     3120

uuuaaacacg gcucagacuu aaaacuaaga aacuucaacu uuuuccacga acuaauggag     3180

ggcgauaaga gucaaggcaa agucgucgau caacaaacag gugaguuaau aacaacaaga     3240

gaugaaguag cuaagagcuu ugacagacua cuaaacauga aauauauguu aguaaguaaa     3300

gaaguucaug acaggucaga ucaauuauac ggagccacaa uaguaaccgc aaaagaaucc     3360

ggcaaacuua caucaccuau agaaauaaag aaaaacagac ccgucgaucu auacggcgcc     3420

uacaccaaug gaacaagugc guuuaugaca auaauaaaau ucacuggaaa uaaaccgaaa     3480

uacaaaguua uaggaguucc cacaacaagc gcagucggau uaaagagagu uggaaaaccu     3540

ggaucugaau ccuacaacca agaacuucau agaauuauca aaucaaaucc caaaguuaag     3600

aaagacuucg agauaguggu cccacauguu aguuauuccc aacuaauagu agauggugac     3660

ugcaaauuua cguuagcaag ugacacauau caacauccag caacucagcu aguuuuaagc     3720

aaagagucaa uggaaauuau agcagacgau uuuaaauucg uaaaagaaaa ccccgcaacc     3780

gcagacgaac aauuaguaag agucuuugau gaaauaguua accaaaugaa ucauuauuuu     3840

acaauauucg accaaagauc aaaucggcaa aaaguaacaa aagcacgaga caaauuugug     3900

ucuuuaccca cggaaaguga auacgaagga gccaaaaaga cacaaguagg uaaaacagaa     3960

guaaucacua accucuuaau gggccuacac gcaaaugccg cacaagguga ccuaaaaguu     4020

cuaggucuua gcacauucgg auucuuccaa ucaaguggag gauugaauuu gucugaagau     4080

gcaaugauag uuuaucaauc cccaaccggg cuauucgaac gaagaauaug ccuaaaagau     4140

auauga                                                                4146


<210>  5533
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5533
augaagaaau cauacgcaau aggcauagac auaggaacca acucaguugg gcacugcguu       60

auguacacag aaaauuauaa aguaccaagc aagaaaauga aaguguucgg uaacacugaa      120

aaacgauaua uuaagaaaaa ucuacuaggc acccuccucu uugaugaagg aaacacagcu      180

gaaaacagaa gacuaaaaag aacagccagg agacguuaca cuagaagaag aaaucguauu      240

cuauaucuuc aagaaauuuu cgcagaagaa auaaauaaaa uugacgacag cuuuuuccag      300

cgacucgacg auaguuucuu aauagucgaa gauaaacaag gaucaaaaca ucccauauuc      360

ggaacauuac aagaagaaaa agaauaucau aaacaauucc ccaccaucua ccauuuacgc      420

aaacaacuag cugauucuuc acagaaagca gacauacgac uaaucuacuu agcgcuagca      480

cacauaauaa aauacagggg acacuuucua uucgaaggag aucuaaaauc agaaaacaaa      540

gacguucagc auuuauucaa cgauuuugua gaaauguucg auaaaacggu ugaaggguca      600

uacuuaucug aaaaccuacc gaauguagca gaugugcuag uagaaaaagu aaguaaaagu      660

agacgguugg aaaacauucu acauuacuuc ccgaacgaga aaaagaacgg acuuuuuggu      720

aacuuccuug cgcuagcauu gggccuacaa ccaaauuuua aaacaaacuu ugaauuagca      780

gaagacgcca aaauacaauu uaguaaagaa accuacgaag aagauuuaga ggaacuucuc      840

ggaaaaauug gcgacgauua cgcagacuug uuuaucgcaa cgaaaucacu cuacgacggg      900

auucuucuag cuggaaucuu aucuacaacu gacucaacca caaaagcacc uuugucaucu      960

uccaugguaa acagauacga agaacaucag aaagaucuag cccuuuuaaa agacuuuauc     1020

cgacaaaacu uaucagauag uuauaaagaa uuuuucaacg auacauuaaa ggauggguac     1080

gcaggauaca uagaaggaaa aaccacacaa gaaaacuuuu auagauuuau aaagaaggca     1140

auagagaaaa uugaaggauc gaacuauuuu auugacaaaa uagauaggga agauuuucuu     1200

agaaagcaac gaaccuucga caauggauca auaccacacc aaauacacuu acaagaaaug     1260

cacgcaauca uacgcagaca agcagaguuc uauccauucc uaguagaaaa ccaagacaaa     1320

aucgaaaaga uucuuacauu ccguauuccg uauuacguug guccauuagc aagaggaaag     1380

ucagaguucg cguggcugaa aagaaaauca gacgagaaaa uaagacccug gaacuuugau     1440

gaaauggucg acaaagaaac cucugcagaa aacuucauaa cucgaaugac aaauuaugac     1500

caauauuuac cagaucaaaa agugcuaccu aaacauuccc uacuauauga aaaauuugca     1560

guauacaacg aacucacuaa agugcgauau guuacggaac aaggaaagag cuucuuuuuc     1620

gacgcaaaca ugaaacaaga aauauucgau ggcgucuuua aaguauaucg aaaagucaca     1680

aaagaaaaac uuauggauuu cuuaggaaaa gaauuugacg aguucaggau agucgaucua     1740

augggauugg auaaagauaa caaaucuuuc aaugcaucac ugggaacaua ccaugaucuc     1800

aaaaagauag ucuccaaaga ccuacuagac aacccugaaa augaagacau ccucgaaaau     1860

guaguacuca cgcucacgcu auucgaagau agagaaauga uaagaaaacg ccucgaaaaa     1920

uacaaagaug uacuaacaga agaacaacga aagaaacuag aaagacgaca cuacacggga     1980

uggggaagac uaucagcaaa acucauaaau ggaauacgaa acaaaaccac acguaaaacc     2040

auacuagauu auuuaauaga cgacggcaca ucaaacagga acuucaugca acuaauucac     2100

gacgauacac ucucauucgu agaugaaauu agacuugcac aaggaucugg agaagcagaa     2160

gauuaucgaa cagaagucca aaacuuagcc ggcucucccg ccauaaagaa agguauacuu     2220

caaucccuaa aaauuguaga cgaacuuauu ggaguaaugg gauauaaucc agaacauguu     2280

guugucgaaa uggcaagaga aaaccaauuu acaaaucaag gcagacgauc aagccaacaa     2340

cgauacaaga aaaucgaaaa ugcaaucaaa aacuuagaca guaacuugaa cucaaaaaua     2400

cuaaaagaau acccaacaaa caaccaagca cuacaaaaug auagacuauu uuuauacuac     2460

uuacaaaacg gcaaagauau guauaccgau gaagaauuag auaucgauca acuaucacaa     2520

uaugauauag aucauauaau accccaagca uuuauaaaag augacucacu ugauaauaaa     2580

guacuaacaa aaagcgcgaa aaaccgaggc aaaucagaug augucccauc auuagaaaua     2640

guacacaaaa agaaaaauuu uuggaaacaa uugcuugaca gccaacuaau aucacaacga     2700

aaauucgaua aucuaacuaa agcugaacga ggcggcuuaa caaacgaaga caaagcaaga     2760

uucauacaaa gacaguuagu ugaaacccgc caaauaacaa aacacguugc uagaauccuu     2820

gacaccagau ucaauacaaa auuggacgac gccgguaaca ggauaagaga cccaaaaguc     2880

aauauaauaa cccugaaauc cagucuagua agccaauuca gaaaagacua ucaauuauac     2940

aaaguuagag aaaucaacaa cuaucaccau gcccacgacg ccuacuugaa ugcaguaguc     3000

gccacagcac uacuuaagaa guauccccaa uuagccccug aguucguaua cggcgacuac     3060

ccaaaauaua acucguacaa aucaagaaag ucagcuacug aacgcguccu auuuuacagu     3120

aauauuauga acuucuuuaa acgaguugug aaaagcagua agacaggaac uguuaaaaua     3180

cggccuauaa uagaaguaaa caaagaaacc ggagaaauag ucugggauaa aaagagcgau     3240

uuucgaaccg uccggaaggu guuaucuuac ccucaaguaa auguagucaa aaagacagaa     3300

guacaaaacc acgguuuaga ccgaggaaaa ccaaaaggau uuuauaaugc caaccuauca     3360

ccaagaccua aagaaggauc aguugaaaau cucguaccag ccaagcaauc guuugauacu     3420

aaaagguacg gagguuaugc cggaauaagc aauagcuacg caguacuugu aaaugguaua     3480

auagaaaagg gcaagaagaa auccaaagca gaaguaaccg aguuccaagg aauaagcaua     3540

uuagcuagaa aagauuucga gaaaaacccc aaacaguacc uacugaaccu cggauacaaa     3600

gauaucaaaa gcauaauaaa acucccaaaa uauucauugu uugaacuuga aaacggaucu     3660

cgcagaaugc uggcaagcau ccuaucaaca aacaauaaac gaggcgaaau ccacaaggga     3720

aacgaaaugu accuccccga caaauuuguc accuuacuau accaugcaau gcgaguuaau     3780

agaacacucg aaccaggcca caagaaauau gucgaaaccc acagacaugu cuuugacgaa     3840

cuccuaacuu acauacuuga auacaaugag aaaauagugg gagcaaaagc uaacgggaaa     3900

agaauaguug aagcguacag cgcacgaaaa gacaccgaca accucgaaga acucugcaac     3960

ucauucauca accuauuaaa ucuaacagcg uuagguagug cuguagauuu ugaguucuug     4020

ggaacaaaaa uaccacggua cagagauuau acaccaagcu cacuccuaaa agcaacauua     4080

auacaucaau cagucacagg acucuaugaa acacguaucg acuuaucuaa auuaggcggu     4140

gacuga                                                                4146


<210>  5534
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5534
augaagaaaa uuauaggcuu agaucuaggg accacaucaa uaggaugggc auacgugcau       60

gaagcagaaa gugcgcaaga acgaucugaa auaguuagau uaggagcaag aguagucccu      120

cuaacaacag augaacaaga ugaguuuaag aaagguaaaa guaucacaac caaugccgca      180

cgauuaagua aacgaaguuu aagaagaaau gcugauagau uccaacuacg aagagaugaa      240

uuggccggug ucaugaaaga agcaggaugg aucgacgaaa caacuguacu agcagaacaa      300

ggcccuagaa guaccuuuga gacuuuaaga uuaagagcaa aggcagcguc cgaggaaguc      360

acuuuagccg aguuugcacg cauacuacuc caaauuaaua gaaaaagagg uuacaaaagu      420

aaccgaaaaa cggacggaga ugaagaagga gcucuaauug augggaugga uguaaguaaa      480

caacuaaaug cauccggaca aaccccugga gaauggguuu uagcaaggau gcaagaagga      540

aaugcuguua uuccugcauu cuacuguagu gacuuaagug aggaacuaga cagaauuugg      600

aggaagcaac aagcaucaca ucaagcgugg augaccgaag aacugcauac agucuugaga      660

caugcagaug guaggucagc uaucaggcua auggacaaag cuggaguggu accagacuca      720

guuaauguaa aagaaaggcg cagaguuaug uaugaauggc gggcagccgc aguacgggac      780

ugucuaccag cggguagagu cgcagcagca uuaaugcaag uaaguaaaga gaucgggaga      840

gccgacucuu acuugggaag gauaggcgac agaagcaaag aacuauacuu ccauaggcaa      900

acuguaggac aauauuuaug gaggcuaaua ugugacaauc cuaaccaauc auuaaagaac      960

uuaguguucu auaggcaaga cuaccaacac gaauuugaaa cucuaugguc aauucaaaca     1020

aaauaucacg uagaacuaac agaagaucuc cgaaaacgug uaggaagaau aauauuuuuc     1080

caaagaccgc ucaaaagcaa gaaaggucua guuaaucgau gugaauuagu ucacuauagc     1140

gucugugaaa acagcgggga ugaucuaacc gaaaaagucg auguaggacc aaaaguaugu     1200

ccuaaauccu cuccacuuuu ucaggaauuu aaaguuuggc aaguccuaaa uaacguagag     1260

guuauucauc cuucaacaca agaggcaaga agacucgaaa uggaagaaaa agaacuacuc     1320

gccagcgagc ugcuuuucag agaccgaaug aagaaaauag acgugcugaa auuacuauuu     1380

ucgaauugga gagaucuaga ucuaaauuac aaagagcuag aaggcaacag aacuauggca     1440

gcuuucuaua ggguauauga acagguaaua gcucaaacag gacauggaga cuaugauuuc     1500

gcaaaaaugc gagccgcaaa agucaaagag cucgcagaag guguuuuugc uggccuaggc     1560

uuugaaguag gccuacuuac auucgaugca gaacuagaca guccugcuua ugaacaacag     1620

ccuugcuaua gacuuuggca cuuacuauau ucauaugaag gagauaaauc ggccacaggc     1680

aacaaaugcc uauuaaagaa acuaugucgg uuaacaggaa ugaacgcuga auugguagga     1740

cuauucaugc aaauaagauu uucaggagau uauggcagcc uaaguacaaa agcaaugaga     1800

aggauucuac ccuaccuuaa agcaggaaau acguaugaug uggccugcga auacgcuggu     1860

uuuagacauu ccgcccguuc auuaacccga gaagaauuag cacaaaaaga auacaaagac     1920

uaucuagauu uacuuccacg uaacagcuua aggaauccag ugguagagaa aauacuaaac     1980

caaaugauac auguaguaaa cggacuaucc ucuacauaug gaaaaccgga ugaaauucga     2040

gucgaaaugg caagggaauu aaagaaauca gcgagagaaa gagaggaaau gacaagcgca     2100

auuuccaaaa caacaaaaga aaaugcagaa auaacagcca uauuacaaag agauuucggu     2160

uugcgcuaug ucagccguaa ugauauauua agguacaaac uauauuguga auuacggaua     2220

aaagguaacg guuguagaac auuauacucc aacacauaua uaaaccccaa cgaacuauuc     2280

ucaagagaau uugacauuga acauauuauu ccacaaucaa gacuauuuga ugacucauuu     2340

aguaauaaaa cucuagaguc aaggacagcc aauuuggaaa aaggggacag aacagccugg     2400

gacuacguca augaaaaaua uggagcugag agagcagaug aauauagggc ucaaguagaa     2460

gcacuauaca gggacggaac uauaucaaaa gcuaaacgcg auaaacugcu uaugccaggu     2520

gacaaaaucc cggaagauuu cauugacaga gauauaagaa acacacagua uauagcaaag     2580

aaagcacuug aaauauucga agacuuuguu cccucaguag uagcaacuuc uggcaaugua     2640

acagcgcgac uaagagagga uuggggucua gucgauguaa ugaaagaacu gaauuggucc     2700

aaauacgagu cgcaaggaau gguacauaua gaauacgauc gagacggucg accaauucgu     2760

agaauuguug auuggacaaa acggaaugau cauagacacc augcaaugga cgcccuagca     2820

guagcguuca caaaaagaag uuauauucaa uaccugaauc auuuaaaugc cagaaaagag     2880

gcaacauccg uugcguacgc uauacaacac aaagagaugg aacuugaugg uaguaauaga     2940

uggagauuua aaugucccuu uggugauagg ucagcauucc gcagagaagc uaaagcacaa     3000

cuagaacgaa uacugguuuc aaucaaaagu aaaaauaaag uacuaacucc uagugucaau     3060

agaacaaaga aaagaggggg auucaacaag aaaguagagu ggacgccaag aggggcguua     3120

cacaaugaaa caguguacgg aagaauuaga agauacgacg uaaaagaauu aagaguaggg     3180

ucaaguuuua cggcagaagu uaucgguaaa guagcugaua gccgauauag agccgcacua     3240

augcauagac uagcagaauu ugguggagau ccuaaaaagg cuuuuacugg uaaaaacagc     3300

uuggagaaaa auccacuaua uacagaugaa gcucauacgg ccuguguccc gaguaguguu     3360

aaaguaguaa guuuuguuga aauagguacu auaagaaaag gaguggaccc gaaccugaau     3420

guggauaaag uuguagauac acauauuaaa aauauauuac uagcacgccu uucagcuuau     3480

ggaggagacg caaagaaagc auuuuccaau cuggacgcag auccaauaug guuaaauaaa     3540

gaaaaaggaa uagcgauaaa aagaguaaca auuagcgacg auucugaagu uagaccuaua     3600

cacguaaaac gagauaugcg aggcagacuu guaugugacg aacauggacg accuguacca     3660

ucugacuacg uacaacugag aaauaaucau cauguggcca uauucagaga ugcaaauggc     3720

gaguuaaaag agcgcguagu uacauucuuu gaagcaguag aacggguacg acaauuacgu     3780

ucugaagguc gagcagugga aguuauagau aaaaauuaca aaugcgaaga aggauggacc     3840

uuuuuauuuu caaugaaaau aaaugaauac uuuguuuuuc cugacgccgc uucaggauuu     3900

gauccaaggg aaaucgaccu uacggacccu gcaaauuacg cacuaaucuc accucauuua     3960

uuuagaguuc aaaaauuaag cucaaaagac uacuauuuua gacaccauuu agagacuacc     4020

gucaaugaug uuaagcaguu aagggacaua acauggaagc gaauaucauc ucuccaagau     4080

uuagcaggga caguuaaagu acggguaaau cacaucggac aaaucgucgc aguaggagaa     4140

uacuga                                                                4146


<210>  5535
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5535
augguaaaaa aaaaauacgc cauuggcauu gauauaggaa caaacagugu agguugguca       60

auaguaacag acgauuauaa aguaccaucg aaaaaaauga aggucuucgg aaacacugaa      120

aaaagauaca uaaaaaaaaa cuuacuggga acacugcuuu ucgacgaagg caauacugcc      180

gaaaacagga gacuuaaaag aacggcgcgc cguagauaca cccgucguag aaaccgaauc      240

cuauacuuac aagagauauu cgcugaagaa auaaauaaaa uagacgauag cuucuuucaa      300

agguuagaug acaguuucuu aauugucgaa gauaaacagg gaucuaaaca cccuauauuc      360

ggaacucuuc aagaagaaaa agaauaccau aaacaauucc caaccauuua ccaucuaaga      420

aaacaacuag cagacucauc gcaaaaagcc gauauccgcc ucaucuaucu agcacuagcc      480

cacauaauaa aauacagagg acacuuccua uucgaaggag accucaaauc agaaaauaaa      540

gauguacaac auuuauuuaa ugauuucguc gaaauguuug acaaaacagu ugaaggaucc      600

uaccuaucug agaaucugcc aaacgucgca gacguucucg ucgaaaaagu uagcaaaagc      660

agacgacugg aaaacauacu acauuauuuc ccaaaugaaa aaaaaaacgg cuuauucgga      720

aauuucuuag cuuuagcacu aggguuacaa ccgaacuuca agacaaacuu ugaacuagcu      780

gaagacgcua aaauccaguu cuccaaagaa accuacgagg aggaucuaga agaauuacuc      840

ggcaaaauug gagacgacua cgcagacuua uuuauugcaa ccaaaaguuu auaugacggc      900

auacuacuug ccggaauacu cucaacuaca gacucuacaa cuaaagcacc cuuaucuagc      960

aguaugguaa acagauauga agagcaucaa aaagaucuag cccuacuaaa aaacuuuaua     1020

caucagaauc uuucagauuc cuauaaagaa guauucaacg auaaauuaaa agauggcuau     1080

gcaggauaca uagaagguaa aacgacacaa gaaaauuucu auagauucau aaaaaaagcc     1140

auagagaaaa uagaaggcag uaacuacuuc auagacaaaa uagacagaga agacuucuua     1200

cgaaaacaac gaacauucga uaacgguucu aucccacauc aaauccaccu acaggaaaug     1260

cacgcaauca uacgacgaca agcagaauuu uauccguucu uaguagaaaa ucaagacaag     1320

auagagaaaa uacuaacauu ccguauaccg uacuacguag gaccccuagc uagaggaaaa     1380

uccgaauucg cauggcuaaa cagaaaaucu gacgaaaaaa uucgacccug gaacuucgau     1440

gaaaugguag auaaagaaac auccgccgaa aacuucauaa cacguaugac aaacuaugac     1500

caguaucuac cugaucaaaa aguacuacca aaacauucac uauuauacga aaaauucgca     1560

guauauaaug aacuaaccaa agucagauac guaaccgagc aaggaaaguc uuuuuuuuuu     1620

gaugcuaaua ugaaacaaga aauauuugau agaacguuua aaguauaccg uaaaguuacc     1680

aaagaaaaac uaauggacuu ccugagcaaa gaauucgacg aauucagaau aguagaccua     1740

cuaggcuuag acaaagacaa caaaagcuuc aaugcgucuu uaggcacaua ucaugaucug     1800

aaaaaaauag ucagcgaaga cuuccuagau aacucagaaa acgaagacau cuuggaaaau     1860

guaguauuaa cacuuacauu auucgaagac agagaaauga ucaggaaacg acuagaaaag     1920

uacaaagaug uuuuaacaga agaacaaaga aaaaaacuag aaagaagaca uuacacagga     1980

uggggccgac uuagugcuaa auuaauuaau ggcaucaggg acaaaguaac acguaaaacc     2040

auacucgauu auuuaauuga cgauggaaca ucgaauagaa acuucaugca acugauaaau     2100

gacgacacau uaagcuucgu ggacgaaaua agacuugcuc aaggaucagg aaaagcugaa     2160

gacuacagag cagagguaca aaaucuugcc gguagcccag cuauaaaaaa agggauacuc     2220

caaagccuaa aaaucguaga cgaacuaaua gaaguaaugg guuacgaucc cgaacacauu     2280

guuguggaaa uggcucggga aaaccaauuu acaaaccaag gucgaagaaa cucacaacaa     2340

agauauaaaa aaauagaaaa ugcaauaaaa aaucucgacu caaauuuaaa cucaaaaauu     2400

cucaaagagu aucccacaaa caaccaggca cuacaaaacg acagacuguu cuuauacuau     2460

cugcaaaacg gaaaagacau guacacagau gaagaauuag acaucgauca acuaucacaa     2520

uacgauauag accacauaau accacaggcc uucauaaaag acgauagcuu agauaauaaa     2580

guauuaacaa aaucugcuaa gaaccgcgga aaaucugaug acguaccgag ccuagagauc     2640

guauacaaaa aaaaaaacuu uuggcaacaa cuguuggauu cucaauuaau cucgcaacgc     2700

aaauucgaca accucacaaa agcggaacgu ggcggauuaa caaaugaaga uaaagcuaga     2760

uucauacaaa ggcaauuagu cgaaacacga caaauaacaa aacacguugc acgcauauua     2820

gacaccagau uuaacacaaa acuagacgaa gcaggaaauc guaucagaga cccuaaagua     2880

aauaucauaa uacucaaauc aaauuuagug ucccaauucc gcaaagauua ucaacuauau     2940

aagguuaggg aaauaaauaa cuaucaucau gcgcacgacg cauaucugaa cgcaguaguc     3000

gccacagcau uacucaaaaa auacccacaa uuagccccag aauuugucua cggugaguau     3060

ccaacauaca auuccuacaa uagcaggaaa agcgcaacgg agaaagucuu auuuuauuca     3120

aauaucauga acuucuucaa aagaauaguu guccacucaa aaacaggaga aguucgaaua     3180

agaccaguaa uagaagucaa uaaagaaacu ggagaaauag uuugggauaa aaaaucaaau     3240

uucagaacag uaaggaaggu uuuaucguac ccacagguaa acgucgucaa aaagacagag     3300

guacagaauc acggauuaga ccgcgggaaa ccuaagggau uuuacaaugc aaauuuaucc     3360

ccucgaccaa aggaagguag uguugaaaac uuaguaccug ccaaacaaag uuuugauacu     3420

aaaagauacg ggggauaugc aggcauuagc aauucauacg cgguuuuagu caacggcaua     3480

auagaaaaag gaaaaaaaaa aagcaaagcc gaaguaaccg aauuccaagg aaucagcaua     3540

uuagccagaa aagauuucga uaaaaaucca aaacaguacu uauuaaauuu gggauauaaa     3600

gacaucaaaa guauuauaaa auuaccaaaa uauucucucu ucgaacuaga aaaugguucg     3660

cgucgaaugu uagcaucaau acucucaacc aauaacaaac guggagaaau acacaaagga     3720

aacgagaugu auuuaccaga uaaauucguc acucuucucu accaugcaau gaggguaaac     3780

agaacguuag aaccaggaca caagaaauau gucgaagcgc accgacaugu auuugaugaa     3840

cuacuaaccu acaucuuaga auauaaugaa aaaauagucg gggcuaaagc aaauggaaaa     3900

cgcaucguag aagcauacag ugcaagaaag gacaccgaca accuagaaga acucugcaac     3960

uccuucauca accuauuaaa ucuaacagcu cucggaucag cgguagauuu cgaauucuua     4020

ggcaccaaaa uaccaagaua ccgagacuac acaccaagcu cacuacuuaa agcgacacua     4080

auccaucaau caguaacugg acuauacgag acaagaauag accugucaaa acuaggagga     4140

gauuga                                                                4146


<210>  5536
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5536
augguuaaaa aaaaauaugc cauaggcauu gauauaggaa caaacuccgu cggauggagu       60

gucgucacag augacuauaa gguucccuca aaaaaaauga aagucuuugg aaacaccgaa      120

aaacgauaua ucaaaaaaaa ccuacuagga acauuacuau uugaugaagg uaauacggcc      180

gaaaauagac gcuuaaaaag aacagccaga cguagauaca cacgacgacg aaaucgaaua      240

cuauacuuac aagaaaucuu ugcagaagaa auaaacaaaa ucgacgacuc auuuuuccaa      300

aggcuagacg auucauuucu aauuguugaa gauaaacaag gauccaaaca cccaaucuuc      360

gguacauuac aagaagaaaa agaguaccac aaacaguuuc caacaaucua ccaccuccga      420

aaacaauuag cggacucauc acagaaagcc gacauaagac uaaucuaucu agcauuggca      480

cacauaauaa aauauagagg ccauuuucuc uucgaaggcg accuaaaaag ugacaacaag      540

gacgugcaac auuuauuuaa ugaauuugua gaaauguucg acaaaacagu agaaggcucc      600

uaucucagug aaaacauacc agauguagcg gacaucuuag uagaaaaagu aucaaaauca      660

agaagacuag aaaauguauu acaauauuuc ccaaacgaaa aaaaaaaugg auuacuaggg      720

aauuuuuuau cccuagcguu aggacuacaa ccaaacuuua aaaccaacuu ugaauuagcc      780

gaagaugcca aaauacaauu uuccaaagag acuuaugaag aagaucucga agagcuccua      840

gguaaaauag gggacgacua ugcagaucug uuuauugcaa cgaaaucauu auacgacgga      900

auucuccuag cuggaauccu cucaacaaca gacuccacua caaaagcacc acuuaguucc      960

ucuaugguca aucguuauga agaacaccaa aaagaccuag cacuacuaaa agauuuuaua     1020

agacaaaacc uaucagauuc auauaaagaa guguucaaug augcgcuuaa agacggguac     1080

gcuggauaca uagaaggcaa aacaacacaa gaagauuuuu auaaauucgu gaaaaaaaca     1140

auugaaaaaa uagaagguag ugauuacuuu aucgacaaaa uagauagaga agauuucuua     1200

agaaaacaaa gaacauuuga caacggaucg aucccucacc agauucaccu aaaagaaaug     1260

caagcaauaa uaauaagaca agcaaaauuu uacccauucc uagaagaaaa ucaggauaaa     1320

auagagaaaa uacuaacauu uagaauuccu uauuacguag gcccuuuagc ccgaggaaau     1380

ucagaguucg ccugguuaaa uagaaagucc gacgaaaaaa uuagaccaug gaacuuugau     1440

gagauaguug acaaagaaac aagugcagaa aauuuuauaa cacgaaugac uaauuacgau     1500

caauaucuac cggaccaaaa agugcuaccc aaacauucac ugcuauauga aaaauucgca     1560

guauauaaug aauuaacuaa agucagguau guuacagaac agggaaaaag cuucuuuuuu     1620

gaugcuaaua ugaaacaaga aauuuuugac ggagucuuua aaguauacag aaaaguaaca     1680

aaagaaaaau ugauggauuu uuuagguaaa gaauuugaug aauucaggau aguugaucuc     1740

cuuggcuuag auaaagauaa caaaucuuuu aacgccaguc uaggaacaua ccacgauuua     1800

aaaaaaauag uaucuaaaga ccuacuugac aacccugaaa augaagauau ccuagaaaau     1860

guagugcuaa cgcuaacacu auuugaagac agagaaauga uacggaaacg ucuggaaaag     1920

uauaaagaag uucuuacaga agaacaacgc aagaaauuag aaagaagaca uuacacaggu     1980

uggggcagau ugucagccaa guugauaaau gguauacggg auaaaguuac ccgaaaaacu     2040

aucuuagacu accuaauaga cgacggaaca ucaaauagaa auuuuaugca acugauaaac     2100

gacgauacgc uaaguuuugu ugacgaaauu agacuagcuc aagguucugg agaagcggaa     2160

gauuaccgag cagaaguaca aaaccucgca gguaguccag cuauaaaaaa gggaauuuua     2220

caaucauuaa aaauuguuga cgaacuaauc gaaguaaugg gauacgaacc agaacacauc     2280

guaguagaaa uggccagaga gaaccaauuu acuaaccaag gacgaagaaa uucucaacaa     2340

cgauacaaaa agauagagaa cgcuauuaaa aaucucgacu caaaucuaaa uucaaaaauu     2400

cuuaaagagu acccaacuaa caaccaagcc cuccaaaaug acaggcuuuu ccuauauuau     2460

cuacaaaaug gaaaagauau guauacagau gaagaacuug acauagacca acucagucaa     2520

uacgacauug aucacaucau cccacaagca uuuauuaaag augauucgcu ugacaacaaa     2580

gucuuaacaa aaucagcuaa aaauagaggg aaauccgacg auguaccauc acuugaaauc     2640

guauauaaaa aaaaaucguu cuggaaacaa cuccuugacu cacaauugau cucgcaacgu     2700

aaauucgaca auuuaacaaa agcugaaaga ggcggauuaa caaacgaaga uaaggccaga     2760

uucauacaaa gacaauuggu agaaacacgc caaauaacua agcacguggc cagaaugcug     2820

gauacacguu uuaacacgaa auuagacgaa gcuggaaacc guauacgaga uccgaaaguu     2880

aauauaauaa cauuaaaaag uaaccuaguc ucacaauuuc guaaagauua ccaacuauac     2940

aaaguuagag aaauuaacaa cuaucaccau gcacaugacg cauaccugaa cgcuguugua     3000

gcaacagcau uauuaaaaaa auacccacaa cucgcaccag aauuuguuua cggagaauau     3060

cccacauaca auucauauaa uucacgaaaa agugccaccg aaaaaguauu auuuuauucc     3120

aacauaauga acuuuuuuaa acggauagua guacacucca aaaccggaga aguacgaaua     3180

aggccgguaa uagagguaaa caaagaaacc ggagaaauag ucugggacaa aaaaagugau     3240

uuucgaacug uucgaaaagu acuaucuuau ccacaaguaa auguaguaaa aaaaacugaa     3300

guacaaaauc auggacucga uaggggaaaa cacaaaggcu uuuacaacgc uaacuuaagu     3360

ccucgcccaa aagaaggaag cgucgaaaau cucguacccg ccaaacaaag cuucgacacc     3420

aagcgauacg gaggauacgc cggcaucagu aacucuuaug caguccuagu aaauggaauu     3480

auugaaaaag gaaaaaaaaa aucaaaagcg gaaguaacag aauuccaagg aauaucaaua     3540

cucgcgcgca aggacuuuga uaaaaacccc aaacaauauc ugcuaaaucu uggauacaaa     3600

gauaucaagu ccauuaucaa auuaccaaaa uacucuuugu ucgaacuaga aaacggaagc     3660

agaagaaugc ucgcgucaau uuuaucaacu aacaacaaac guggcgaaau ccacaaaggg     3720

aacgaaaugu accuaccgga caaauuugua acccuauuau aucacgcaau gaggguaaau     3780

agaacuuuag aaccuggaca uaaaaaauau guugaaacuc aucgccacgu guucgacgaa     3840

cuccuaauau acauauuaga auacaaugaa aaaauaguag gagcaaaagc gaauggcaaa     3900

cgaaucguag aggccuacuc agcaagaaaa gacacugaca accuugaaga acuauguaau     3960

ucauucauca aucucuuaaa uuugaccgca cuuggcucag caguagauuu cgaauuucua     4020

ggaacaaaaa uaccccgaua ucgggauuac acgcccucau cacuccugaa agcaacuuua     4080

auccaucaaa gugucacugg auuguacgaa acccgcauug acuuaucaaa acuaggagga     4140

gacuga                                                                4146


<210>  5537
<211>  4146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5537
auggucaaaa agaaauacgc uauaggaaua gacauuggga cuaauucggu cggcuggucu       60

guaguaacug acgacuauaa gguuccaagc aaaaaaauga aagucuucgg aaauaccgag      120

aaaagauaua ucaaaaaaaa uuuacuaggc acucuauuau ucgacgaagg caacacagcu      180

gaaaaucgua gauuaaaaag aacagcuaga cguagauaca cucgaagaag aaacagaauu      240

uuauauuugc aggaaauuuu cgcagaagaa auuaacaaaa uagacgacuc uuuuuuccaa      300

cgucuugaug auagcuuucu uauugucgaa gacaaacagg gcaguaagca cccaauauuu      360

ggaacuuuac aagaagaaaa agaauaccau aaacaguuuc ccacaauuua ucaccuacga      420

aaacagcuag cagacucaag ucaaaaagca gauauccgau uaauauaccu agcauuagcg      480

cacaucauaa aauaucgagg acauuuucua uucgaaggag acuuaaaaag ugaaaacaaa      540

gacguacagc acuuauucaa cgacuucguc gaaauguuug acaagacugu ugaaggcuca      600

uaucuuucag aaaaccuacc aaauguagca gauguacuag ucgaaaaagu aucaaaaucu      660

cgacauuuag aaaauauuuu acacuauuuc ccaaacgaaa aaaaaaaugg acuguuugga      720

aauuuccuag cauuagcauu aggacuacaa ccaaacuuca agacaaauuu ugaacuagca      780

gaggaugcua aaauacaauu uucaaaggaa acauaugaag aagaccuaga agaacucuua      840

ggaaaaauug gcgaugacua cgcagaucuu uucaucgcga caaagagucu cuacgacggc      900

auucuucugg cugguauauu aagcacuacu aauucaacua cgaaagcccc acuaucguca      960

agcaugguca auagauauga agaacaccag aaagaccuag cguuauuaaa aaacuucauc     1020

caucaaaauc uaucugauag uuauaaagaa gucuuuaacg acaaauuaaa agacggguac     1080

gccgguuaca uagaagguaa aacuacccaa gaaaauuuuu accgcuuuau aaaaaaagca     1140

auagaaaaaa uagacggauc aaauuacuuc aucgaaaaaa uugaaaggga agacuucuua     1200

agaaaacaaa gaacauucga caauggaucu auaccacacc agauucaucu acaagaaaug     1260

caggcaauca uacgaagaca agcgaaauuu uacccauucu uagaagaaaa ucaagacaag     1320

auugagaaga ucuuaaccuu cagaauacca uacuauguug gaccccucgc cagaggaaaa     1380

agcgaguuug caugguuaaa uaggaaauca gacgaaaaaa uaagaccuug gaauuuugac     1440

gaaauggucg auaaagaaac cuccgcaaaa aacuucauaa cacgaaugac aaacuaugau     1500

caauaccuac cugaucaaaa gguauuacca aaacauucau uacucuacga aaaauuugcc     1560

guauacaacg aacuaaccaa aguccguuau guaacagaac aaggaaaauc auuuuucuuu     1620

gacgccaaua ugaaacagga aauuuucgau ggaguauuua aaguuuauag aaaaguaaca     1680

aaagaaaaac uaauggauuu ccuuggaaaa gaauuugacg aauuuagaau agucgacuua     1740

cuaggccucg auaaagaaaa caaaucuuuc aaugcaagcc uaggaacaua ucaugaucuu     1800

aaaaaaauag uaucaaaaga cuuccucgac aacagugaaa acgaagauau ccucgaaaau     1860

guaguacuga cacucacauu auuugaagau cgagaaauga uccgaaaacg ccuagaaaaa     1920

uacaaagaca uucuaacaga agaacaaaga aaaaaacuug aaagaaggca cuauacagga     1980

uggggucgac ucagcgccaa acuaaucaau ggaaucagag acaaagucac acgaaaaacg     2040

aucuuagauu aucuaauaga cgaugguacu ucaaacugca acuucaugca auugauaaau     2100

gacgauacau uaucauucgu ugaugaaauu agacuagcuc aaggaagugg ugaagccgaa     2160

gauuaccgag cugaaguuca aaaucuagca ggcagccccg ccauaaaaaa aggaauccua     2220

caaucacuaa agauuguaga ugaacuuauc gagguaaugg guuacgaccc agaacacauc     2280

gucguggaaa uggcuagaga aaaccaauuc accaaccagg gccgacgaaa cagccagcaa     2340

agauauaaaa aaauagaaaa cgcaauaaaa aaccuagauu caaauuugaa uucaaaaaua     2400

cuaaaagaau auccaaccaa uaaccaagcu cugcaaaacg aucgacucuu uuuauauuau     2460

cuacaaaacg guaaagacau guacacugac gaagaacuag auauagacca acuaucacaa     2520

uaugauaucg accacauuau accacaagcc uuuaucaaag augacucgcu ggacaauaaa     2580

guacuuacaa aaucggcaaa aaaccgaggg aaaucagacg acguacccuc auuagagauc     2640

guauacaaaa aaaaaucauu cuggaaacaa uugcuugacu cccaacuaau uucgcagaga     2700

aaauucgaca accuuacaaa agcagaaaga ggcggucuua cgaaugaaga caaagcacgu     2760

uucauacaaa ggcaauuagu ugaaacaaga cagauaacua aacacguagc ucggauguua     2820

gacacccgau ucaauacaaa acuggaugaa gccggcaaua gaauaagaga cccuaaagug     2880

aauauuauaa cacuuaaaag caaccuaguu ucacaauuua gaaaagacua ccaauuauau     2940

aaaguccgag aaauaaauaa uuaucaucac gcgcaugacg cauaccuaaa cgcuguugug     3000

gccacagcac uacuuaaaaa guauccgcag cucgcgccug aauuuguuua uggcgaauac     3060

ccaacauaua acagcuacaa cagcagaaaa ucagcaacug aaaaaguuuu auuuuauuca     3120

aacauaauga auuucuucaa gagaauagua guacauucaa aaacaggaga gguaagaauu     3180

agaccaguca uagaaguaaa caaggaaaca ggcgaaauag ugugggauaa aaaaucagac     3240

uuucguacag uaagaaaggu auuauccuau ccccaaguga acguaguuaa aaaaacagaa     3300

guacaaaauc acgguuuaga uagaggaaaa ccaaaaggau ucuauaacgc aaaccucucu     3360

ccacgaccua aggaaggcag cguagaaaac cuaguaccag caaaacaauc auucgauacu     3420

aaacgauacg guggcuaugc cggaaucucc aacucuuaug cuguuuuagu gaauggaaua     3480

auagaaaagg gaaaaaaaaa aucaaaagcc gaaguaacgg aguuccaagg aaucagcaua     3540

uuagcaagaa aagacuuuga caaaaauccc aaacaauauu uauuaaauuu aggauacaaa     3600

gauauaaaau cuauaauaaa acuaccuaaa uacucauuau uugagcuaga aaacggcagc     3660

cgaagaaugc uagcuucuau auuaucaaca aacaauaaac gaggagaaau ucauaaagga     3720

aacgaaaugu aucuuccuga caaauuugua acauuacuau accacgcaau gagagucaac     3780

agaacucucg aaccagggca uaaaaaauac guagaaacgc accgucacgu auuugaugaa     3840

cuguuaaucu auauacuaga auacaaugag aagauuguag gagcaaaggc uaaugggaag     3900

agaaucgugg aagcguauuc cgcaagaaaa gacaccgaua aucuagaaga acuuuguaac     3960

agcuucauaa accuucuaaa ccuaacagcu cuuggaagcg caguugauuu cgaauuccuu     4020

ggaacaaaaa uaccacggua uagagauuac acaccauccu cauuauuaaa agcuacacuc     4080

auacaccaau caguaaccgg ucuuuacgaa acgagaauag auuuaagcaa acucggagga     4140

gacuga                                                                4146


<210>  5538
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5538
augaagaaaa uagugaauua caaucuaggc cucgauaucg gcacuucguc aauaggauau       60

gccgcaaucg acgaccaagg ucgaccugua agacaccaaa acaaaaccgu aaugggaguc      120

cgacuauuua aagaagguaa aacagcagaa aguacaagga uauucagagg uacccguagg      180

cgacucaguc acagacauug gaggcuugga uacuuagaca gaauauuuga uaaugagaua      240

gccaaggugg augaaaauuu ucuaccgcga cuaaaagcca gcaaaagggc acgaucugau      300

gaaaaacgaa guuuuagagg aucacuucuc uucccagaga cuggcgauga ugcauauuac      360

caaaaauauc caacaauaua ccaucucaga uaugcauuaa uaacccagaa aaagcaauuu      420

gacauccguc ugauuuaucu ugcccuucac cauaugauaa aauauagagg caauuuucua      480

gacacaaccc cgguauccgc uuucaacaua cacgauauag aucuuacaga ucaauuugca      540

ucgcuaaacg auuguuacca acuauuagau auggacuucg aguuccaauu ugacuugaau      600

aauaucgaac aaguccaaaa acuccuucuu gaugaaaccu uaucgaaaac uaaaagacaa      660

gcggcaguua gcaaaguccu cguaaugagc acaggcaaua aggcauuuga ugaccaacaa      720

aacggcauag cuucagaguu caucaaagcc cucuugggag gaccauuuga ccugucgaaa      780

auuuuaaauc uaucaguggc cgauguaaaa uccuggaaac uacacaugac ugauaaagac      840

guagacgaua cacuaaacag ccuaguugaa gaauuagacg aaaaucacaa gcaaauugua      900

aaucuacuuc aaaugauaua cagucaaguc augauguuag gucuacuuaa uggacaacaa      960

ucuaucucug caucaaugau ugaaagauac gacgaccaug cuaaacacug ggccgccuua     1020

aagaaacucc uacccgcccu aggugaacaa gaacaaucca cgaucauuca ugcguauacg     1080

gcuuacacag gacacucucg uacuaaacaa cuauccaggg ccgaguucua uaccaaagua     1140

aacucuauuc uuaagaaaca ugaaaacauu gcagaagcaa ccgguuugau ugaacuaauu     1200

gauaggaacg aguucaugcc aaaacaaaga gacgcaguaa acggaguuau cccucaccaa     1260

uuacaccaac gagaauuaca acuaauaaua caaaaccaac aaaaauacua uccauuucua     1320

gcagcaaaag cauacccgga ugccgaugaa acaaugauag aacaguugcu uagcuuucgu     1380

guuccuuauu acgcaggacc acuugucgac cgugacuuua uuaacgaaag cagugaccaa     1440

gaacaagcua ugcaaaauac aaaauuuagu uggcuaaaac gaagagaagc aggagaaauu     1500

acaccaugga auuucgaaca aaaagucgau guucaagaga cagcuaauag auucauuaaa     1560

cgcuuaacaa ccaaagacac auaucugaug acagaagacg uacuaccgaa agaauccuua     1620

aucuaucaaa gauuugaagu ccuaaacgaa cuaaaugcaa uaaggauaaa cgauaggaga     1680

cuaacugcag aaaauaaaca acuagcauuu caacaauuau uuauggcagg caacacuaaa     1740

aauguaagca uuaagaaauu caggaacuuu guauuugauu cauuaaaauu uccagagaaa     1800

gugaaaauaa caggauucgc aggccaacac aacuuccuag ccaaacuaag cacauaucau     1860

gauuuuaaaa agauauucgg agaaagagua gaucacacug acuuacaacc agacuucgaa     1920

agaauaguug aauggugcac aauauucgag gaucgacaca uauauagaga aaaacucaaa     1980

gaaauacaau ggcugagcca aagcgaaaua gaccaauuag uacucauacg aaaucauggu     2040

ugggguaggc uauccaaacg ccuucuaacc ggaauuagag acagugaugg acgcacugua     2100

auggaccaau ugugguuugg uacugauaau uuuaugagaa uaauaucuca aucagacuuu     2160

gccgcagcaa uagcaaacga aaauagaacg caacuuaacc aacacggcuu agaagacguc     2220

uuagacuccg cauuuauguc uccacaaaac aagaaagcaa uaagacaagu cuuaaaaguc     2280

guagaugaca ucgaacaaac ccugggaugu cccccaaaga aagucaugau agaauuuacc     2340

cgcucaccag auaaaaagaa acaacgaacc gucacgaggc uugagaaagu acaacaagca     2400

uacaaagccg uagcggacca auuagcgggc caaucgaucg ucucagagcu ugaccaauac     2460

gcuaacacuc auucaaaacu gucagacacc uauuaccuuu auuucaugca auuagguaga     2520

gaucuguaua gugggaaacc cauagacauc gacuccccca ugcuagacga cgaucacauc     2580

cucccuaggg cauuuaugaa agacaauuca cuagacaaca gagcucuaac agaaaaaccg     2640

auaaauaaua caaaagccgc caaauucccc ucuaaaguau uugggcaaca aaaagaagua     2700

acagacuuuu ggaaacuucu acguagaugc gggcuaaugu cuaauauaaa agaaaaacac     2760

uuauaucuag acccggacaa uguggacaaa uggacccgaa gaggauuuau ucaccgacag     2820

cuaguugaga caucacaggu aauaaaaauc guugcuaaca uuuuaaacga ccgauaucaa     2880

caaucacaaa cagaaguccu agaaguaccu gccaggcuag gcacagaacu acgcgaaugc     2940

uucggauggu acaaagucag agaaguaaac gauuaccauc acguauuuga cgcauacuua     3000

gccgcauuua uaggaaacua ucuauauagg agauacccca aauuacaacc uuacuuugua     3060

uacggcaauu uugccgaacu uaacaaagcu caauuaaaga auauaaaaag uuuuaauuuu     3120

cuauacaaca uuacagacaa caaacgagug guaaaagaca uugaaacggg acaaacauua     3180

ugggaugauc aaauaagaca acaaauacaa cauauauacg aauacaaaua uauguuaaca     3240

ucccacgaag ccuuuacaca acacaaagaa cuuuauggac aaacaauaua uccuagacga     3300

gaauuuaaac caggcacacu aaaagccaua aaacaagauc aaccucaaga gcuguacggu     3360

ggauacaaaa aucuaacagc ugcuuacaug ugcauaguaa aaauucaagc acguaaacga     3420

cgcgcagcug aauacagagu cguacgaguu gcaacagagu cucucagcgc acuacaacaa     3480

gcaaaacguu uaggcguuca agcguaugaa cgaugucuca ggcaagaauu aguacgaaau     3540

aacgaaaucc ucagaacaaa cgccgauccc aauucagcac cacgugaguu cgaaguacuu     3600

cuagaucgug uacuauguca ucaaaugauc auauccgaug gacaaaaaua ccuacucggg     3660

ucucacgcau auaaaauaaa uuguaugcaa uuaguacuaa guaacaauuc aaugaagauc     3720

cucuccaccu ccauaguuaa auuaaaacaa augacaaacg aaccguuagu acaaguauac     3780

cgcgaaauuc uagacgucgu caauaaauau uucacgcuau acgacgucaa uucauucagg     3840

gaaaacuuaa aucaauucuu cgauaaauuu agaacauuac caauuaccaa cgaauacgaa     3900

agcucuaagu caggaaagaa acuaauacac agaggucaau uagaaacacu cacccuaauc     3960

cuacggggcu uacaugcuaa cagugacaaa guucaauacg gugaucaaag uacacuacua     4020

ggcgguuuuc aaacaaaaga cgguauaaaa uuaagcaaag auucaguaau ucaauacacu     4080

ucaccagccg gccuaaaugu ccguacuguc aaauuaagag aucuagagca aaaagaagcu     4140

cagaaauga                                                             4149


<210>  5539
<211>  4149
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5539
augguagacg agaaaauauu uuggggacuc gauaucggga ccgacuccgu ugguugggcg       60

guaacaaacu cagaauacaa acugaagaaa uacaaaaaua aucuuaugug ggguguacac      120

cuguuugacg aagcaaaaca gucagcugaa cgcagaucgu uucgaaccgc uagacgccga      180

uuagacagaa gaaaacaaag aauuauacua uuacaggaau uuuucgugag agcaguuugc      240

gaaaaagacg aaaacuucuu ucgucgauua aaagaaagcg cauuauuacc agaagaugca      300

gaacacagga cgaacaauau auucuuugac gacccugauu auacugacaa agauuauuuu      360

gaggaauacc caacuauaca ucacuuaauu ugugaacuaa uggaaucuaa agaaccacac      420

gauguaaggc uaguauacuu agcaugcgua uacuuacuag cacaucgggg acacuuucua      480

cuaccgguau cggaggauga cauaucaaaa guaacagaau uugaaccucu guaugaauca      540

uucuacaaag cacuggaaga aaaauuagac gacgaaccgc cauuugaccg aucggcugac      600

gacuuugcag aaauacuaaa aucacauaaa acuguaagcg ccaaaaacaa agauuucgau      660

aaacuacuau uuggagguaa aguaaaaacg uacgacaaug aaaacauauc guauucagca      720

uuaaucaaac uuuuaucugg aggaacagaa aaacuaucaa aauuuuucgc caacgaagaa      780

uacacagacc uagaaaaaga cucagugugc gugagaaaug cugacuuugg agauacacua      840

gaaauguuag agggacaaau cgaugaauua gauuuugcau uacucaaauc aguuaaauca      900

cuguacgacu ggagccuccu aguggauaua cuagaaggaa aaucccuaau cucggaagca      960

aagaaagaca aauaugacga acacggauau gaucuugaug cacucaaaua ccuauuuaga     1020

gaauacuuga caaaagacga cuauaaugaa auguucaaag aaguaucagg aaaacagaau     1080

uaugcgucau acguuuacaa ugcaaguagc gacaaaacaa gagacucaaa auacaagaaa     1140

ugcucacaaa aagauuuuug caaauucacu aagaaauucu uaucaaaaau aaaaccaaau     1200

gaaaaagaua aaccgugcuu agauaaauua cucgaaaaau gugaacagaa cucguuaugc     1260

ccaaaacaag uaacaaccga caacagagua auaccauacc aauuguacua cguagaacuu     1320

aagaaaauuu uagaaaaugc auguggcuac uuaccauucc uaaaugaaag agaugaauau     1380

ggcacagugg cagacaaaau ucuuucaaua augaaauuua gaguaccgua uuauguagga     1440

ccccuaguag acagcaagaa auccccaaau gcauggcuag uaagaaaacu cgaugguaaa     1500

auaacaccau ggaauuuuac agauaugguc aaugaagaug aaucagaaaa ugccuucaua     1560

agacguauga caugcaaaug uacguacguu gcaggacagg acguacuacc uaaauauuca     1620

uuacuauauu caaaauucuc cguacuaaau gaaauuaaua auauaaaacu aaacggagaa     1680

ccaauaagug uacaaaccaa acaagaaaua uauacagagc uauucgagag gaacaaaagu     1740

cguguaucga agaagaaaau uagagauugc cuaauaaguc acgguuaugc agcugacuca     1800

gacgaaguca cagguauaga ugauauagcc aaaucagcgu uaagauccua ccaugacuuc     1860

aagaaaaugc ucucgaacgg aauacuaaca gaacaacaag uagaggaaau aauagaacac     1920

auuacuguca cuacugauaa uauaagacuc aagaaauggu uaaaaacgca auuuccuaug     1980

cuagcagacg aagauguaaa auacaucaca aaacugaaau auaaagauua cgggagauua     2040

ucgcgauguu ucuuagaaga cguacuccca guagacacaa aaacaggaga agccgaaagc     2100

gacaaaaaua ucaucacaau gcucugggaa acaaacgaaa acaucaugca guuacuaagc     2160

aguaaauaca gauacuccga gaauauugag cacaugaaca gacaauacua cgcacuaccu     2220

gaaaaccaca aaucaaugag ugaacgacuu aaagacaugu auguaccaac agcaguaaga     2280

cgggcaguga cgagaacauu ugacauuguc aaagaauuaa agaaaaucca agggagagac     2340

ccagacaaaa uuuucauuga aauggcaaga ggcacuggug aaacuccaaa aggaaaaaga     2400

accaauucaa gaaaagacca aauuuuagaa cauuggcacg gcuuagacaa uaaagauaua     2460

aacgaucuca agaaaucggg aauaugggaa cacuuggaca caauugauga ugccaaacua     2520

aggucagaca aauacuuccu cuauuucaug caacuaggcc gauguaugua cacagagaaa     2580

ccgauaccgu ucgaagaggu agaaaaugaa cauaaaugga auauagacca uauauggcca     2640

caggccaaaa uaaaagauga cucacuugac aacaaaguac uugucaguag uaacgaaaau     2700

ggcaagaaau cagauagcua cccaauaucc gacgacauac gacacagcau ggcaggacua     2760

uggcacucuc uuuacaagaa agguuuaaua agugagaaga aauaccaaag gcugacaagg     2820

ucaacaccuu uuaccgauga ugaguuaagc ggcuucauag cucgucaauu aguugagacc     2880

agacagucua caaaagcagu cgcuacguua uuaaaagaac aguucccaaa cacugaaaua     2940

guauacguaa aagccgguuu agucucugau uucagacaag aaauggguau guuaaaaugu     3000

cgagaaguaa augacuuaca ucacgcaaaa gaugcuuauu uaaauauugu uuuagguaac     3060

guauacaaua caagguucac aaaagaccca uuaaauuucg uaaagaauaa cgagaaauac     3120

ucaauaaaaa uauuccagaa aaacucagau ggcaagaaaa ccggaguaau uacaagaaaa     3180

guagagagag guggagaagu ugcaugggau cccgagacau cauuugcuau cguccgaaaa     3240

augaugagca aaaauucaau acgguacguu agauaugcau acaugagaaa aaguggccaa     3300

aaugguggcu uuuucgauca aaacccgcua cguaaaggaa aaggcauggu cagcauuaaa     3360

cacaaucuau cuauagaaaa guacggcgga uaugacaaug ccacuauuag cuucuuuucg     3420

cuagucucaa uaaaagauuu uggggucaua uuacuagccg uagaacuauu aucagcaaac     3480

aaauuucuaa agaauauauc agaggcuaaa gaauacgcag cagacaaacu aucaaaagaa     3540

cugaacguca aaauagugcc aaacgaugug gccuucccac uaaauaauag guacaacguc     3600

gugaaaauca acgcaguguu ggaacuagac gguuaccggg ucaugaucgc cgcaaaacaa     3660

aacaguagua ucaaguuugu aucaaaugaa ucacuaauac uaaguucgca acauauggca     3720

uauguaaaga aaauaacgaa auuuaucgag aaaagaaaac aagauaaggu auguaugaua     3780

aaugccuuuu cagggauaag cauagaagac aauauagaau uauaugaugu gcuaguaagc     3840

aaaugcaaca ucaaaccauu caauauaaug uaugagaacu uaggaaucaa auuaucagau     3900

aauagggaca auuucaucaa gaaaagugua gaaaaucaag uauuaauauu gagcgaaaug     3960

guuagguugu uuaaaacagg aagaaaagcc gguguuaauc uagaagaagu aggaggcaga     4020

aagaacucug guguaaaaac auuaaacaca ugccuauaua aaguaaaaga uuacucuaau     4080

auauucuuag uagaucaaag cccaacagga uuaauagaac acaagucccc aaacuuauug     4140

gacuuguga                                                             4149


<210>  5540
<211>  4155
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5540
augaauaaac ccuauucaau agguuuggau auaggcacaa acaguguagg auggucaaua       60

auuacugaug auuacaaagu accagccaag aaaaugagag uauuaggaaa cacagauaaa      120

gaauauauca agaaaaacuu aauaggugcu cuacucuucg augggggaaa cacagcagca      180

gacagacggu uaaaacgaac agcacgucga cgcuacacuc guagacgaaa ccgaaucuua      240

uaccuucaag aaauauucgc agaggaaaug ucaaaaguug acgacucauu cuuccauaga      300

cuagaagauu cauuccuggu agaagaagac aaacgcggcu caaaauaccc aauauuugcc      360

acucuacaag aagaaaaaga cuaucacgaa aaauucucaa cuauauauca uuuaaggaaa      420

gaacuggccg acaagaaaga aaaagcugau uuaagacuca uauacauagc auuagcucac      480

auaauaaaau uucgugguca cuuccucaua gaagacgacu cauucgaugu ucgaaacaca      540

gauauaagca aacaauacca agauuuccuu gaaauauuua auacaacuuu ugaaaacaac      600

gacuugcugu cacaaaaugu agacguagaa gcaauccuca cagauaaaau caguaaauca      660

gccaagaaag aucgaauacu ugcacaauau cccaaccaga aaaguacagg caucuucgca      720

gaguuccuaa aauuaauagu aggcaaccaa gcagacuuua agaaauauuu caaucuagaa      780

gacaaaacac cguuacaauu cgcgaaagau ucauacgaug aagaccuaga aaaucuuuua      840

ggccaaauag gagacgaguu cgcugaccua uucuccgccg ccaagaaacu auaugacuca      900

guuuuacuaa gcggaauacu uacuguuauu gaucuaagua ccaaagcccc acuaagcgcg      960

agcaugauac aaagauauga cgaacauagg gaagaccuaa aacaacugaa acaauucgua     1020

aaagcaaguc uacccgaaaa auaccaggaa auauucgccg acucaucaaa agaugguuau     1080

gcaggauaua uagaaggaaa aacgaaucaa gaagccuucu auaaauaucu caguaaacua     1140

uuaacgaaac aagaagacuc agaaaauuuc uuagagaaaa uuaaaaacga agacuuuuua     1200

cguaaacaaa gaacauuuga uaauggaucc auaccacauc aaguacaccu cacugaacuc     1260

aaagcaauaa uuagaagaca aucugaauac uacccuuucc ucaaagaaaa ccaagacaga     1320

auagagaaaa uacucaccuu caggauuccg uacuacaucg gaccacuugc uagagaaaaa     1380

ucagacuuug ccuggaugac aaggaaaaca gacgauucga uccggcccug gaauuucgaa     1440

gauuuagugg acaaagaaaa aucagcagaa gcauuuauuc auagaaugac caauaaugac     1500

uucuaucucc cagaagaaaa aguucugcca aaacacaguc ugauauacga aaaauuuaca     1560

guauauaacg aauuaacaaa aguacgauau aaaaacgaac aaggagaaac auacuucuuc     1620

gacucaaaca uuaaacaaga aauauucgac ggaguuuuca aagaacaucg aaaaguaagc     1680

aaaaagaaac uucucgacuu uuuagccaaa gaauaugaag aauuuagaau aguagacgug     1740

auaggacuug acaaagaaaa uaaagccuuu aaugcuaguc ugggaacaua ccaugacuua     1800

gagaaaauuc uugauaaaga uuuucuagau aauccagaca acgaauccau auuagaagau     1860

auuguacaaa cacucacacu cuucgaagau cgagaaauga uuaagaaacg auuagaaaau     1920

uacaaagauc ucuuuacuga aagccaacuu aagaaacuau accguagaca cuacaccggc     1980

uggggacgcc uauccgcaaa acucauaaac ggaauacgag acaaagaauc ccagaaaacg     2040

aucuuagacu aucuaauaga cgauggccgc uccaacagaa acuucaugca auugauaaau     2100

gacgacgguc ggucaaacag aaacuucaug caacucauaa acgaugacgg cuugagcuuc     2160

aaaucaauaa uuucuaaagc acaagcagga agccauagug acaacuuaaa agaaguugua     2220

ggagaacucg caggcagucc agcaaucaag aaaggcaucc uacaaucgcu uaaaaucguc     2280

gacgaauuag ucaaagucau gggauaugaa ccagaacaaa uaguaguaga aauggcaaga     2340

gaaaaccaaa caacgaacca aggaagaaga aacaguagac aacgauacaa acuacuagac     2400

gauggaguca aaaaucuagc gucagaccuu aaugguaaua uacugaaaga auaccccacg     2460

gauaaccaag cacuucaaaa ugaaagguua uucuuauauu accuccaaaa uggaagagau     2520

auguauacag gagaagcacu cgauaucgac aaucuaucuc aauacgacau ugaccauaua     2580

auaccccaag ccuucauaaa agaugauuca auugacaaua gaguacucgu aucaucugcu     2640

aaaaaccgag gaaagucaga ugacguuccg ucuuuggaaa uagucaaaga cugcaagguc     2700

uucuggaaga aauuacuaga cgcaaaacuu augucacaaa gaaaauauga caacuuaaca     2760

aaagcggaaa gaggcggacu cacgucagac gauaaggcaa gauuuauaca acgucagcua     2820

guugaaacca gacagauaac caagcacgua gcuaggauuc uggacgaaag guucaacaac     2880

gaacuagaua gcaaaggaag acgaauccgu aaaguuaaga uaguaacacu caaaagcaac     2940

cuaguaucaa acuuuagaaa ggaauuugga uucuauaaaa uaagagaagu caauaauuac     3000

caucaugcac augacgccua uuuaaacgcc guaguagcaa aagcaauccu uacaaaauau     3060

ccucaacuag aaccugaguu cguauauggc gacuacccua aguauaauag uuauaagacc     3120

agaaaaucag caacagaaaa acuauucuuu uacucgaaca ucaugaacuu cuucaaaacc     3180

aaaguaaccc uugccgacgg uacaguugua guaaaagacg acauagaagu aaauaaugac     3240

acaggugaaa uagucuggga caagaagaaa cacuuugcaa caguaagaaa aguacuaagu     3300

uacccgcaaa auaauauagu caagaaaaca gaaauacaaa caggaggauu cucgaaagaa     3360

aguauucuag cacacggaaa uuccgacaaa cuuaucccaa gaaaaaccaa ggacaucuau     3420

cuagacccua agaaauaugg uggauucgau ucaccaauug uugccuacag cguccuagua     3480

guagcagaca uaaagaaagg aaaagcacaa aaacuaaaaa caguuacaga acucuuagga     3540

auuacuauua uggagcgauc cagguucgag aaaaacccca gcgccuuccu ugaaucaaaa     3600

ggauaccuca acauacgcgc agauaaauua auaauacuuc ccaaauacag ucuauucgaa     3660

cucgaaaacg gaagaagacg uuuauuagcc agugcgggag aacuacaaaa ggguaaugaa     3720

cuagccuuac ccacgcaauu uaugaaauuu uuauaccuag caucucgaua caaugaauca     3780

aaagguaaac cugaggaaau agagaagaaa caagaguuug uaaaccaaca uguuuccuac     3840

uuugacgaca uuuuacaacu aauaaacgac uuuagcaaaa gaguaauccu agcagaugcu     3900

aaucuggaga aaauaaauaa auuauaucaa gauaacaagg aaaacaucag uguugaugaa     3960

cucgcaaaca acauuauaaa ucucuuuaca uucacaagcc ucggagcacc agcggccuuc     4020

aaauucuuug acaaaauagu agaccguaaa agauacaccu cgacaaaaga aguauuaaac     4080

ucaacccuaa uacaccaguc aauaacugga uuauaugaaa caaggauuga cuuaggaaaa     4140

cugggugaag auuga                                                      4155


<210>  5541
<211>  4155
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5541
augaaauugc gugguauuga ggaugacuac ucuauaggau uagauauggg gacgaguucg       60

gucggauggg ccgucaccga cgaaagggga acguuggcac auuuuaaacg uaaaccuaca      120

uggggaucaa gguuauuucg agaagcccag acugcagccg uugcacgaau gccaaggggg      180

caaagaagaa gauauguucg gagaaggugg agacuagauu uauuacaaaa acucuuugaa      240

caacaaaugg aacaagcuga uccggacuuc uuuauacggc uaaggcaauc aagguugcua      300

cgagacgaca gagcugaaga acacgcggac uauagauggc cacucuuuaa ugacuguaaa      360

uuuacugaaa gagacuauua ccaacgauuu ccgacuauau aucauguaag aucaugguua      420

auggaaaccg acgaacaagc cgauauacga cugauauauu uagcacuaca uaacauagua      480

aaacacaggg gaaacuuucu uagagaggga caaagucuau ccgcaaagag ugcaagaccc      540

gaugaagcau uaaaucacuu aagagaaaca cucagaguau gguccucaga acgcggguuu      600

gaauguagca uagcagauaa cgguuccaua uuagcaaugu uaacucaucc ggacuuaagu      660

ccuucugaua gaaggaagaa aauugcaccu uuauucgaug uuaaauccga cgaugccgcg      720

gccgacaaaa agcuaggaau ugcgcuagcu ggagcuguaa uaggcuuaaa aacagaauuu      780

aaaaacauuu uuggugauuu uccuugugaa gauaguucga uauaccugag caaugaugaa      840

gcaguugaug caguacgguc ugcuugucca gaugacugug cugaacuuuu cgacagguua      900

ugcgaaguau auagcgcaua uguccuacaa gggcuucuca guuaugcacc aggacaaacc      960

auaagcgcca auauggugga aaaauacaga cgguauggag aagauuuagc cuuacugaag     1020

aaacuuguca aaauuuacgc cccagaccaa uacagaaugu ucuucucagg agcaacuuac     1080

cccgguacag gcauuuauga ugccgcacag gcaagaggau auacaaaaua uaaucugggu     1140

cccaagaaaa gugaauacaa accuucggaa ucuaugcaau augacgacuu ccgaaaagca     1200

gucgagaaac uauucgcuaa aacagaugcg agagccgacg agagauaucg aaugaugaug     1260

gacagauucg auaaacaaca auuucuuaga cgacuaaaaa caucagacaa cgguucgauu     1320

uaccaccaau ugcauuuaga agaacuaaaa gcaaucguag aaaaucaagg aagauuuuau     1380

ccuuuucuaa aaagagacgc agacaagcua gugucccuag uaucauuuag gauaccguau     1440

uacguaggac cauuauccac aagaaacgcu agaacagauc aacauggaga aaacagauuu     1500

gcuuggagcg agagaaaacc ugguaugcaa gaugaaccga uauuuccaug gaauugggaa     1560

ucuauuaucg auagaucaaa aagcgcugaa aaauuuauac uacgaaugac ugguaugugc     1620

acauaucuuc aacaagaacc uguuuuaccu aaaaguucau uauuauauga ggaguucugc     1680

guacuuaaug aacuaaacgg ugcucacugg ucaaucgacg gugaugauga acauagguuu     1740

gacgcugcgg aucgugaagg aauaauagaa gaguuauuuc gacgaaaaag aacuguuucu     1800

uauggcgaug ucgcaggaug gauggaacgc gaaagaaacc aaauaggagc acacgucugc     1860

gguggacaag gcgaaaaagg auuugaaucc aaauuaggaa gcuacauuuu cuuuuguaaa     1920

gacguuuuua aaguggaaag auuagaacaa ucugauuauc ccaugauaga aaggauaaua     1980

uuauggaaca cacuuuucga agacagaaaa auauuaagcc aaagauuaaa agaagaauac     2040

ggaagucgau uaucagcuga acaaauaaaa acuauaugca aaaagagguu uacaggcugg     2100

ggaagacuca gugaaaaauu cuuaacaggc auuaccguac aagucgauga agacaguguc     2160

ucgauaaugg acguucuacg agaagggugu ccuguaucag ggaaaagagg uagagcaaug     2220

gucaugaugg agauccuacg cgaugaagaa cugggauuuc aaaagaaagu agaugacuuu     2280

aaucgagcau uuuucgcuga aaacgcacaa gcacuaggag uaaacgaauu accuggguca     2340

ccagcgguac gacggucguu aaaccaauca auuagaauag uugacgaaau agccucaaua     2400

gcuggcaaag caccagcuaa uauauuuaua gaaguaacuc gggaugaaga uccuaaaaag     2460

aaaggucgaa gaacaaaaag gagauauaac gacuugaaag acgcacuaga ggcauuuaaa     2520

aaggaagacc cugaacuuug gagagaguua ugugaaacag caccaaacga cauggaugaa     2580

agauuaagcc uauauuucau gcaaagggga aaauguuuau acagugguag ggcgauagac     2640

auucaccagc uaucaaacgc ugguauauau gaaguggauc acauaauucc uagaacauac     2700

guaaaagaug acagccuuga aaacaaagcc uuaguauaca gggaagaaaa ccaaagaaag     2760

accgacaugc uauugauaga ucccgaaaua agacgacgaa ugagcggaua uuggcgaaug     2820

uugcacgaag caaaacuuau uggugauaag aaauuucgaa accucuugag gagcagaaua     2880

gaugacaaag cauuaaaagg auucauagca cgacaauuag uggaaacagg ucaaauggua     2940

aaacucguga gaucacuauu agaagcgaga uauccggaga ccaauauaau aagcguaaaa     3000

gcauccauaa gucaugaucu aagaacagcg gcagagcuag ucaaauguag agaagcaaac     3060

gauuuucauc augcgcauga ugcauuccuu gcaugcagag ugggacuauu uauacagaaa     3120

agacacccgu gcguauauga aaacccaauu ggauugucgc aaguaguaag aaauuaugua     3180

aggcaacaag cagacaucuu uaaaagaugu aggacuauuc cgggaucauc uggcuuuaua     3240

guaaacucgu ucaugacauc aggauuugau aaagagacgg gagaaaucuu caaagaugau     3300

ugggaugcag aagcugaagu ugagggaauu agaagaaguu uaaauuucag acaauguuuu     3360

auaucgagaa ugcccuuuga agaucauggu guauuuuggg augcaacuau auacagucca     3420

cgagcuaaga aaacagcugc acuuccucua aaacaagguc uaaacccguc uagauacggg     3480

uccuuuucua gagaacaauu cgccuauuuc uuuauuuaua aagcucgaaa uccaaggaaa     3540

gagcagacgc uuuuugaauu ugcucaagua ccugugaggu uaagugcaca aauacgacaa     3600

gacgaaaacg cauuagaaag guacgcuaga gaauuagcua aagaucaagg gcuagaauuu     3660

auaagaauug agagaucaaa aauauuaaag aaccaacuaa uugaaauaga uggcgauagg     3720

cuauguauaa caggaaaaga ggagguucga aacgcaugug aauuagcauu ugcacaagac     3780

gaaaugaggg uuauacguau guuagucagu gaaaaaccag ugucucgcga gugcguaauu     3840

agccuuuuua aucguaucuu acuacauggu gaucaagcau cucgccgacu caguaaacaa     3900

uuaaaauuag cacugcucag cgaagcauuu ucagaagcaa gcgacaacgu gcaaaggaau     3960

guaguacuag gcuugauugc uauuuuuaac gggagcacaa auaugguaaa ucucagcgau     4020

aucggcggca guaaauuugc cgguaauguc agaaucaaau auaagaaaga auuagcauca     4080

ccaaaaguaa auguacaucu gauagaucaa ucaguaacag gaauguucga acgcagaaca     4140

aaaaucgguc uuuga                                                      4155


<210>  5542
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5542
augacaaaac cguauucaau agggcucgac auagggacua acucagucgg augggcagua       60

auuacagaug acuauaaagu acccucaaag aagaugaaag uacuuggaaa uacaagcaaa      120

aaauacauua aaaaaaacuu auuaggugca uugcuauuug acucaggaau aaccgcugaa      180

gggcgucgac uaaaaagaac ggcuagaaga agauacacac gaagacgaaa ucgcauuuua      240

uaccuucaag aaaucuucuc aaccgaaaug gcaaccuuag acgaugcuuu cuuccaacgc      300

uuagacgauu ccuuccucgu cccugaugac aagcgagauu cuaaauaucc uauuuucgga      360

aacuuaguag aagaaaaagu uuaucacgau gaguuuccaa caauauacca ucucaggaaa      420

cacuuagcug auucaucgaa aaaagcagac cuuagacucg ucuaccuagc auuagcacau      480

augauaaaau accgaggaca uuucuuaaua gaaggagauu ucaauucaaa aaauaaugac      540

cuacaaaaaa auuuucaaga uuuucuagac acauauaacg cuauauucga aagugaccua      600

agccucgaaa acuccaaaca auuagaagaa auugucaaag acaaaauuuc caaaucggca      660

aagaaagaua gaauacuaaa auuauuccca ggagaaaaaa acagcgguau auuuucagaa      720

uuucuaaagc uaauagucgg uaaccaagcc gacuucagaa aauacuuuaa ucuagacgaa      780

aaaaccagcc uacaguuuuc uaaagaaagu uacgaugaag aucuagaaac gcuucuuggc      840

cauauaggag augauuauuc agauguguuu cucaaagcca aaaaauuaua ugaugcgauc      900

cuacuaucug gaauccuuac aguaaccgac aauggcacag aagcuccacu caguuccgca      960

augauaauga gauacaaaga acacgaagaa gaucuugccc uucuaaaagc auauaucaga     1020

aauauuaguu uagaaacuua caaugagguc uuuaaagaua acaccaaaaa cgguuacgca     1080

ggcuacaucg augguaaaac aagccaagaa gauuuuuaug ucuaccuuaa acgauuacua     1140

gcaggucuug aaggggcuga cuauuuccuu gagaaaauug accgagaaga uuuccugaga     1200

aagcagagaa cauucgacaa uggauccaua ccauaucaaa uuuauuuaca agaaaugaga     1260

gcaauauuag acaaacaagc uaaauuuuac ccauuucuag caaaaaacaa agaaaggaua     1320

gaaaaaauau uaacuuucag gauacccuau uaugugggcc ccuuagcgcg uggcaacucu     1380

gauuuugcuu ggucaauacg gaaacgaaac gaaaaaauaa cuccauggaa uuucgaagau     1440

guaaucgaua aggaaaguag cgccgaagca uucauaaacc ggaugacauc guuugaccua     1500

uaucuucccg aagaaaaagu acucccaaaa cacagucucc uuuaugagaa cuuuaccguu     1560

uauaacgaac uaaccaaagu aagauucauc gcagaaggaa ugagggauua ucaauuucuc     1620

gacagcaagc aaaaaaaaga uauaguccga cuuuacuuua aaggaaaaag aaaaguaacc     1680

gacaaagaca ucauagaaua uuuacacgcc auugacggau acgauggaau cgaacuaaaa     1740

ggaauagaaa aacaguucaa cucaucccuu ucaaccuauc augaccuuuu aaacauaaua     1800

aacgauaaag aauuccucga ugacagcucu aaugaaacaa ucaucgaaga aauuauccac     1860

accuuaacaa uguuugaaga cagagagaug aucaaacaaa ggcuauccaa auuugauaac     1920

auuuuugaua aaucuguauu aaaaaagcuu ucacggagac acuauacugg guggggaaaa     1980

uugagugcaa aacuaaucaa cggaauccgu gacgaaaaaa gcggaaauac uaucuuagac     2040

uauuuaauag augauggcgu guccaaucga aacuucaugc aacuaaucca ugacgaugca     2100

cucuccuuua aaaaaaaaau uaaaaaagca caaaucaucg gcgauaaaga uaauauuaag     2160

caaguuguaa aaucacuacc ugguaguccg gccauaaaaa aaggaauucu acaaucgauc     2220

aaaauaguag augaauuagu caaaguaaug ggucgagaac cagaauccau ugucguagaa     2280

auggcaagag aaaaucaaua cacaaaccaa ggaaaaucaa acucucaaca acgcuuaaaa     2340

aggcuugagg aaagucuaaa gggauuagga uccaagauac uaaaagaaaa uguucccaca     2400

agacuuucaa aaauugacaa uaaugcacua caaaacgaua gauuauauuu auacuauuua     2460

caaaacggaa aagacaugua uacgggagaa gaacuagaca ucgaucgacu aucaaauuau     2520

gauaucgacc auauuauacc acaagccuuu cuaaaggaug acuccaucga caauaaaguc     2580

cuuguuaguu cagcuaguaa cagaggaaaa uccgacgacg uaccgucacu agaagucgua     2640

aaaaaacgaa aaacauuaug guaccaauua cuaaaaucaa aauuaaucuc ucaacguaaa     2700

uuugauaauu uaaccaaagc agaacgaggu ggccuauccc aagaagaaaa agcaggauuu     2760

auucaaagac aacuaguuga aacacgacaa auuaccaaac acgucgccag auuacuggac     2820

gagcgauuua acaacaaaaa agaugaaaac aauagaaccc uaagaaccgu aaagauaaua     2880

acucucaaau cuucuuuggu aucacaauuc agaaaagacu uugaacuaua caagguaagg     2940

gaaauaaacg acuuccauca cgcacaugau gcauauuuaa acgccguagu cgccucagca     3000

uuacugaaaa aguacccuaa auuagaacca gaauucguau auggggauua ccccaaauac     3060

aauucauuua gagaaaggaa gucggccacu gaaaaagugu auuucuauuc aaacauaaug     3120

aacaucuuca aaaaaagcau accgcuggca gacggaacag uaauugacag accacuaaua     3180

gagguuaacg aagaaacagg agaaaguguu uggaauaagg ucgcagaucu caacaccgua     3240

agaaaagugc uuuccuacag ucaaguaaau auaguaaaaa aaguagaaga acaaaaccac     3300

ggcuuagacc gaggaaagcc aaaaggacuc uucaacgcaa aucuaaguag caaacccaaa     3360

ccaaauagua aagaaaacuu agugggagca aaagaauauu uagaccccaa aaaauacgga     3420

ggauacgcug gaauaucaaa uaguuucgca auacuaguaa aaggaaccau cgagaagggc     3480

gcaaaaaaaa aaauaaccaa uguacuggaa uuucagggga uauccauuuu agaccgaaua     3540

uauuacagaa aagauaaguu aaauuuuuua cucgagaaag guuauaaaga uauugaauua     3600

auaauugaac ucccuaaaua uucauuguuc gaacuaagug acgguuccag aagaauguua     3660

gcaucaauac ucagcacgaa uaacaaaaga ggagagauac auaaaggcaa ucaaauauuc     3720

auaucacaaa aauucguaaa auugcuauac caugcuaaac gcauaucuuc cacauuuaac     3780

gaaaaccaca gaaaauacgu agaaaaucac aaaaaagaau uugaagaauu auucuauuau     3840

aucuuagaau uuaaugaaaa uuacguugga gcuaagaaaa auggagaauu acuaaaauca     3900

gcauuucaau cguggcaaaa ccacuccauc gacgaacuau guucaucauu caucggaccg     3960

acaggcagug aacgaaaagg gcuauuugaa cucacgucaa gagguggagc agcagacuuc     4020

gaauucuugg gaguuaagau uccaagauac agagacuaca cgccauccag ccuauugaaa     4080

gaugcaacgc uaauacauca aucagucaca gggcuuuacg aaacacgaau cgaccuaggc     4140

aaacuaggcg aggacuga                                                   4158


<210>  5543
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5543
augcaaacau acuuuggaau gaagaaaaua uuaggauuag aucuuggaac aaacucaaua       60

ggcugggccc uaguaaacga agccaacgau gacaacgaaa aaucuaguau aauaaaacuu      120

ggaguaagag uuaauccucu aacugucgau gaacagucaa auuucgaaaa aggaaaaucc      180

aucaccacua acgcugaaag gacaauaaaa cgcucaauaa gaagaaguuu gcaacguuau      240

aaacugcgaa gagaaaaucu aaucgaaauc cuaaaggaac acaauuuuau cacagaugau      300

accauauuau ccgaagacgg aaacgcaucu acauuugaaa cguacaggcu aagagcaaaa      360

gcagcauccg aaaaaauauc gcuagaagag cuuggccgcg uuuuauuaau gauuaacaaa      420

aaacgagguu acaagucauc cagaaaagca aaaaaccaag aagaaggaca acuaauagac      480

ggaauggaag uagccaaaga acucuacgaa aagaauauca ccccugguca auucguauuu      540

caacuauuaa aacagggagg caaauucauu cccgacuucu accgguccga ucuucaagca      600

gaguuugaca aaauuuggga auuucaaaaa uuauuccaug cugaaauucu aguagaugac      660

uucaaaaaac aguuagaacg aaaaggaaaa cuaaauucaa guaaaauauu ccuuggacgg      720

uuuggaauau acaccgcaga aaauaaagga aaugacaaac gacuccaggc uuaccaaugg      780

agaguagaug cccuuacgaa agaauuaacg aaagaagaau uagcauaugu aauaagcgau      840

uuaaacggga ucauuucaag cucaagcgga uacuugggaa guauaucaga uaggagcaaa      900

gaacuauauu ucaacaacca aacaguagga caauuucuaa uggcacaauu agaccauuca      960

ccacacacaa gucuuaaaaa ucaaguguuu uaccgacaag auuaucuaga cgaguucaau     1020

acacuauggg aaaaccaagc gcauuaccau aaaguacuua ccagcgauuu gaaaagugaa     1080

auuagggaug uaauaauauu uuaucaaaga agacuuaaaa cccaaaaagg cuuaauaagu     1140

uucugcgaau ucgaaaguag gaaaaucgaa guagaaaucg auggcaaaaa aaaaaucaaa     1200

aauguuggau cccgaguaug cccuaaaagc agcccuauuu uccaagaauu caagauaugg     1260

caaauucuca acaacauuga auuacuagaa aaaagcagua aagcaaaaag auuuuuagac     1320

caagaagaaa aagaacuauu auuuaaagaa cuaagcauaa agaguaaaau cucgaaauca     1380

gaugcucuaa aacuucucuu caaaaauuca agagaacucg acuuaaauua caaggaaauu     1440

gaagguaaua gaacgcaauc agcgcuauuc gacgcauaua gcaaaauuau agaauuaagu     1500

ggacacggag aauacgauuu cucaaaaaug gaggccucua auaucauaga acuagugcaa     1560

gaaguauuca ccggauugaa cuauaauacc gaaauacucu auuucgauuc gucauuagaa     1620

gguaaagauu ucgaaaacca accaauguac cgacuauggc acuugcuaua caguuuugag     1680

ggagacgauu ccaaaacagg aaacgaaaaa uugauaaaua aacuaagcga acuauacgga     1740

uucgagaaaa aauacaguau uguccucgca aacguuagcu uccaagaaga cuauggauca     1800

cuaucaacca aagccaucag aaagauacac ccuuauuuaa aagaaggaau acaauacgau     1860

guagcaugcg aauuugcagg uuaccgucau ucaaaaagua gucucaccaa agaagaaauc     1920

gaaaauaaaa uacuuaaaga ccaauuggag auucuaccaa aaaacagucu uaggaaccca     1980

guuguagaga agauauugaa ucaaaugauu aacgugguaa augcccuaaa cgaaucauac     2040

ggcaaaccag acgaaauuag aauagaauua gcuagggaau uaaaaaaaag ugcaaaagaa     2100

cgugaagaac uaaccgcauc aauaggagaa acaacaauaa aacaugaaaa auauagagcc     2160

auacucgaga acgaauuugg uauuucacau guaagcagaa acgauauaau ccgauauaaa     2220

uuauaucuag aacuagaaaa cagaggauac aaaacucuau auucuaauac cuauguaucu     2280

caagaaaaac uguucagcaa agaauuugac auagaccaca ucauacccca aagcugucuc     2340

uucgacgaca guuuuucuaa caaaacccua gaaucaaaau caguaaauau agaaaaaggc     2400

aaugacacag ccuucgauua uguguucgca aaauauggug aagccggccu cucggacuac     2460

auaauacaca ucgaggaauu guucaaagac ggaaaaauuu cuaagacaaa acuaaacaaa     2520

cuaaaaauga aagguaauga gauaccaagc gacuucaucg cccgugauau uagagauaca     2580

caauauauug cccgaauagc aaaaaacaua cuggaagaga cagucagaaa cgucguauca     2640

acuagcggaa guaucacaga uagauuaaga gaagacuggc aacucauaga cguaaugcaa     2700

gaacucaauu gggaaaagua cucagcgcuu ggacucacgg aaauauacga aaauagagaa     2760

ggccauacaa uaagacgaau aaaagacugg acuaagagaa acgaccaccg ucaccacgcg     2820

auggacgcau uaacaaucgc auuuacaaaa agaucgcaca uucaguacuu aaacaauuua     2880

aaugcuagau cugacaaguc aggcucaauc uauggaaucg aaacuaaaga gcucuauaga     2940

gacgaccacg gaaaacuuaa auuuaaacca ccaauaccau uaaaugaauu cagggcugaa     3000

gcgaaaaaac accuagaaaa uacacuaauu ucgauaaaag caaaaaacaa aguagcuacc     3060

auaaauauca acaccaccuc caccaaagaa ggaaaaaaca aaaaacggca auuaacgcca     3120

cgaggccaac uccaccugga aacaauauac ggcucaauac agcaauaugu aacaaaacaa     3180

gaaaaaguag gagcaagcuu uaaugaggag caaauaagca aaguagccaa acaaaaauac     3240

agacaggcau uacugaacag acuccgagaa uucgacaaug accccaaaaa agccuuuacg     3300

ggaaauaaua guuucgaaaa aaacccgaua uacuuagacg uacuacaaac acagaaagua     3360

cccgauaaag uugaaacagu cucaaaagaa auuaucuaca caauucguaa agacauaacg     3420

ccagaacuca aaauagaaaa aguaauagau aaaaagauua aaacaauauu acaaucuaga     3480

cuaaaugaau auuccgguga uuccaaaaga gcauucucca auuugaaaga aaacccaaua     3540

uggcuaaacc aagagaaagg cauugaaaua aaaaggguua caauaucggg aauuucaaac     3600

gcagucgcaa uacaugacaa aaaagacaag aacggaaauc uaauacucga uaacgaggga     3660

aguacccaag caguagacuu cguaaacacc gguaauaauc accacguagc aauauacaag     3720

gacgaaaggg gaaaccuaca ugaaucaguc guaucuuucu ucgaagccug uacacgaguc     3780

aacuuaggug gaccggugau auuuaaaaac uauaacaagu cagacgggug ggaauuucua     3840

uuuagcauga aacaaaacga auauuuuguc uuuccuaaug agaagaccgg auucaacccu     3900

aaugaaauug accuacucga uccagacaac uaucgacuaa ucucacccaa ccucuucaga     3960

guacaaaaaa ucaguucaaa aaacuacgua uucaaucauc auuuagagac aucagccauu     4020

acuggugaaa ccuuaaaaaa caagaaacag cuaucaagug ugacuuauca uuuuauacaa     4080

ucuuugccgc cgauaaaaga uaucguaaag gucagaauaa acaacaucgg ccaaaucguu     4140

ugggucggag aguacuga                                                   4158


<210>  5544
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5544
auggacgaac uucaaaacuu ugaaaaaggc aaaaguauua caaccaaugc agacagaaca       60

gcugggcgaa gcaugcgaag aaacuuacag cguuacaaac uaaggagaga aaacuuaaua      120

gaaguucuaa aagaauacaa uuuuauaacu gaugaaauga uacucagcga aaauggaaac      180

aggacaacuu uugaaaccua cagacugcga gcuaaagcag ccguggaaga gauauccuua      240

gaacaauucg cucguguauu acucaugaua aauaagaaaa gaggauauaa aaguaguagg      300

aaagcuaaag gcgcugaaaa cggaacccua aucgauggaa uggaaguagc gaaaaagaug      360

uaugacgaag gaauaacacc aggagaauug uguuuacaac uucuaacggc aggaaaaaga      420

uacuucccug acuucuauag gagcgauuug caggccgaau uugauagaau auggaacuuu      480

cagaaacagu uuuacccaga uagccuaaua gauaaaguaa aagacgaagu ccgcggcaaa      540

aacaaauccc agacaugggc aauacuagca aaauacuucg uuuggaaaga aguugaaaau      600

ucguggaaug aagaagaagc ccaaacaaga agaguagaaa aggaauaccg ccuaguagga      660

auaaagagag gaguaaagag agaggaacua aaauuagaaa auuuucaaug gagaguaaaa      720

gcauuaagug aacgaaugaa ucccgaagaa uuagcaauag uauuacaaga aauuaacgaa      780

caaauaucaa auaguagugg auaccuagga gcuauuucag auagaagcaa ggagcuguau      840

uucaaccgac aaacaguagg ucaauaccaa auggcggucc uagauaacaa uccgaauaua      900

ggauuacgua acaugguuuu cuacagacaa ggauauuugg gagaguucaa cacuaucugg      960

gaaaaacaag cggaguucca caaggaacua acggaagacu uaaagaaaga aauuagggac     1020

auagucauuu ucuaccaaag aagacuaaaa ucccaaaaag gauuaauaaa uaucugcgag     1080

uucgaacguc gucaaauuga aguugaaauu gauggaaaga agaaaauaaa aaccauaggc     1140

ucaaggguaa ucccccgaag uucgccauua uuucaagagu ucaaaauaug gcaaacgcua     1200

aaugacaucg aagucuccgu acucgggguc aaaaacaaac gaaagaaaca agaugauaau     1260

ucuaccacac ucuuagacuc agcagaaaau auagauucac uaaaauuaaa uguaucuaga     1320

ccuuuagaug cggaugagaa aaguuuauua gccaaagaac uauuuauaag agacaaauua     1380

accaaaucgg augucuuaaa acuacuauuc aacaauccac aaaaucugaa ucuaaauuuu     1440

aagaauauag auggaaacag aacugguagu gcucuauauc aagcauucag caaaauacuc     1500

gaaauuucag gccaugaaag cauaaauuuu aagaaaucag cagaugaaau aguagaacaa     1560

guaaaaacaa ucuuuucagc cuuaggaugg aacacagaag uacuuuguuu cgacucagaa     1620

aaagaacuug auaaacaacc uuacuuuaaa uuauggcacc uacuuuacag uuucgaagga     1680

gacagcacac ccacaggaga uggaaaucua auucagaaaa uagccgaccu uuguggauuu     1740

gaaaaagauu augcgagcau uuuagcaaac auaacguucc aagaagacua cggaaguuua     1800

ucaaccaaag cuauacguaa aauacuacca caucuuaaag acggaaauca auaugacgua     1860

gccugugaau acgcaggaua uaaacauagc aagucuucau ugaagaaaga agaaaucaaa     1920

aacaagguac uaaaagauaa auuagaauua cucccuaaaa acucacucag aaacccagua     1980

guugagaaaa uauuaaacca aaugguaaau guaauaaaug ccaucauaac uaccuauggc     2040

aaaccagaug aaauucguau agaauuagca agagaacuaa agaagaaagc gaagaaaaga     2100

gaagaguuaa caaaaacuau aguagauaca acaaaaacac acgaaggaau acgagcgaua     2160

uuacaaaacg auuucggaau gucacacguu ucgagaaacg ccauaauaag auauaaauua     2220

uaugaagaac uaaaagauaa uggauauaaa acacuguauu caaacaccua uauaccuaaa     2280

gaaaaauuau ucagcaaaga cuucgauauc gaacacauaa uuccacaagc acgauuauuc     2340

gaugacucgu uuuccaacaa aacguuggag gucaaaucaa uaaacaucga aaaaggcuca     2400

aaaacagcau augauuucgu agaagaaaaa uacggcgaac aaggacuaca agaguacuug     2460

aacaggugug aggucuuguu cagggacaag aaaacuaaac uaagaaaauu aaaaaugcaa     2520

gaaagugaaa ucccugaagg uuucauugau agagaccuac gaaauacgca guacaucgca     2580

aagaaagccc uaucaaugcu aaacgaaauc ugcagacgcg uaguugcaac gaccggagca     2640

auaacagaug aauuaagaga agacuggcaa uuagucgaug uuaugaaaga auuaaacugg     2700

gaaaaauaca aaguccuagg gcuaguugaa uauuucgaag auaacgacgg aagauuaauu     2760

ggaagaauaa aagauuggac aaaaaggaac gaucacagac aucaugcuau ggaugcgcua     2820

acaguagcau uuacuaaaga cguauuuauu caguacuuua auaacaaaaa cgccaguuua     2880

aagccugaua caaacgaagc agcaauuaga aacaaguauu uccaaaacgg aaaagcaauc     2940

ccgccaaugu cccuugauga guuccgagca gaagcaaaga aacauuuaaa ugauacuuua     3000

aucaguauaa aaacgaaaaa caaaguagua acaauaaauc uaaauaggac gaaaaagaaa     3060

aacggaauaa auaagaaaca acaacaaacc ccgagagggc aacuucaucu ggaaacagua     3120

uacggauccc acaaacagua cgucguuaaa gaagaaaaag uuaaugcuag uuucgacgcu     3180

gccaaaauag caacaguaaa caaacccgcu uauagggaug cccuucuuag aagauuacaa     3240

gaaaacggua augauuccaa gaaagcauuu accgggaaaa auucacuaga uaaacaaccc     3300

gugugguuag acgcaaaaca aaaugaaugc guacccgaaa aaguaaaaac aguuauguua     3360

gaaacgguau acaccgucag aaagccaaua gaugaaaaau uaaacguaga caaagucgua     3420

gaugcccgaa ucagaaaauu acuucaggac agaauuaaag aauauggaaa cgccaagucc     3480

gccuuugcua aucuagauga aaauccaaua ugguuaaaua aagaaaaagg cauaacaauu     3540

aaaagaguaa guguaagugg aaucucaaag gcacaaucuu uacaugaaaa gaaagauaaa     3600

gauggaaacu uuauauugga caaagauucu aggaggauac cagucgauuu cguaaauaca     3660

ggaaauaacc accacguggc uauuuauaga aaaccuguuu uagauaaaaa gggucaagca     3720

guuuucgacg agaauggaga guuaaaguuc gaguuagaag aaguaguagu cagcuuuuuc     3780

gacgcaguaa cacgaguaaa uuuaucacaa ccuauaauag auaaggauua uaaacgauca     3840

gaaggaugga aauucuuauu uagcaugaaa cagaaugaau auuucguauu cccaaacgaa     3900

aagaccggau uugacccaaa ggaaauagau uuauugaauc cagaaaacua ugcauugauu     3960

aguccgaauc uauuccgggu acagaaaaua gcaagcaaag auuauguauu caggcaccac     4020

cucgaaaccg uaguugccga uagaaaagaa uuacaagaaa ucacauggaa gcgaaucagc     4080

ucgcuuucaa uauuaaacag aauaauaaaa guucgaguaa accacauagg ucaaauagug     4140

augauaggag aauacuga                                                   4158


<210>  5545
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5545
augacaaaac ccuacaguau cggacuugac auaggaacua auuccguagg gugggccguc       60

auaacagaug acuauaaagu accaagcaaa aaaaugaaag uucuggguaa uacaucaaaa      120

aaguacauua aaaaaaaucu auuaggcgcg uuacucuuug acuccgguau aacagcagaa      180

ggcagacguc uuaaacgaac agcuagacgc agauacacga ggcgacgcaa cagaauacua      240

uaucuacaag aaauuuuuag cacugaaaug gcaacauuag augacgccuu uuuccaacgc      300

uuagacgaca guuucuuagu gccagacgac aagagagauu caaaauaucc aauauucgga      360

aaucuaguug aggaaaaagc cuaccacgau gaauucccaa caaucuauca ccuccggaaa      420

uaucuagccg auagcacaaa gaaagcggau cuccgauugg ucuaucucgc acuagcacac      480

augaucaaau auagagguca cuuuuuaauu gagggcgaau uuaacaguaa aaacaacgau      540

auacaaaaaa auuuccaaga uuucuuagac acauacaaug caauuuucga aucugaccua      600

aguuuagaga acucaaaaca acucgaagaa auaguaaaag acaaaauauc aaaaagugca      660

aaaaaagaua gaauucuaaa acuauucccc ggggaaaaaa auucaggaau auucagcgaa      720

uuuuuaaagc ugauaguagg aaaccaagca gacuuuaaaa aauauuucaa cuuagaugaa      780

aaagcacccu uacacuuuuc caaagaaucu uacgacgaag aucuugaaac ccuauuagga      840

uauaucggcg augauuauag cgauguuuuu cuuaaagcua aaaaacuuua cgaugcaaua      900

cuacuaucag ggauacuaac cguaacagac aaugguacag aagcaccauu aucaagcgca      960

augauaaugc gcuacaaaga gcaugaggaa gaucuagcau uauuaaaagc cuacauucgu     1020

aacaucaguu uagaaacaua caacgaagua uuuaaagacg auacaaaaaa uggcuacgcc     1080

ggauacaucg augggaaaac aucacaagaa gauuuuuacg uacaucuaaa aaagcuccua     1140

guaggacugg aaggugcgga uuauuuccua gaaaaaaucg acagagaaga cuuccuucgg     1200

aagcaacgga ccuuugauaa cggaaguauu ccauaucaaa uacacuuaca agaaaugcga     1260

gcaauccucg acaaacaagc caaauuuuac ccuuuccuag cgaagaacaa agaacgcaua     1320

gaaaaaauac uaacauuuag aauaccauac uacguuggac cucuugcccg aggcaacagu     1380

gacuucgcgu gguccauccg caaaaggaau gagaaaauaa caccuuggaa uuucgaagau     1440

gucauagaua aagaaucaag ugcagaagcu uuuauaaaua gaaugacgag cuuugaccuu     1500

uaucuaccag aagaaaaagu acuaccuaaa cauagucugc uguacgaaac auuuaauguc     1560

uauaaugaac uaacuaaggu acgauuuauc gcagaaucua ugcgugacua ucaguuucuc     1620

gacagcaaac agaaaaaaga uauaguucgc uuauacuuua aaaauaaacg cagaguuaca     1680

gacaaagaua uuauagaaua ucuacacgcu auugacgguu augacggcau agagcuaaaa     1740

gguauagaaa aacaauuuaa cucgucauua aguacuuauc augaccuacu aaacauaaua     1800

aaugacaaag aauuccuaga ugacucuucc aacgaagcaa uuauagaaga aauaauacac     1860

acccucacga ucuuugaaga ccgcgaaaug auaaaacaga gauuaucaaa auucgaaaau     1920

auuuuugaua aaucagucuu gaaaaaauua uccagaagac acuauacagg auggggcaaa     1980

cuauccgcca aauuaauaaa ugguauccgc gaugaaaaau cagguaacac aauccuagau     2040

uaccuaauag acgauggcgu aucaaaucga aacuucaugc aauuaauuca cgacgaugcu     2100

uuaaguuuua aaaaaaaaau aaaaaaagcu caaauuaucg gcgacaaaga uaauauaaaa     2160

caagucguuc aagcacuucc agguagaccu gcaauuaaaa aaggaauacu acaaaguaua     2220

aaaaucguag augaacucgu aaaaguaaug gggcgaagac cugaaucaau aguaguagaa     2280

auggcaagag aaaaccaaua caccaaucaa ggcaaaucca acucacagca aagacuaaaa     2340

agacucgagg aaucucucaa agaauuaggg ucuaaaauuc ucaaagagaa uauccccgca     2400

aaacucucaa aaaucgacaa uaacgcgcuc caaaacgauc gcuuauacuu guauuacuua     2460

caaaauggaa aggacaugua uaccggugaa gaauuagaua uugauaggcu aucaaacuau     2520

gauauagauc acauuauacc acaagcauuc cucaaagaua auucaaucga caacaaagua     2580

cuaguuagca gcgcuagcaa cagaggaaaa ucagaugacg ucccaucacu agaaguagua     2640

aaaaaacgaa aaacucugug guaucaacuc cuuaaaucaa aacuaaucuc ucaacgaaag     2700

uucgacaacc uaacgaaagc ugaaagggga ggacuaucac cagaagcuaa agcaggauuu     2760

auccagagac aauuagucga aacgagacaa auaaccaaac acguugcuag acuguuagau     2820

gaacgcuuca acaauaaaaa agacgaagau aacaaaacac uacgaacugu aaaaaucauc     2880

acacucaaau caaguuuagu cucacaauuc aggaaagauu ucgaacuaua caaaguaaga     2940

gaaauaaacg acuuccauca cgcacacgau gccuaucuga acgcagucgu agccucugcg     3000

cuauuaaaaa aauacccaaa acuagaaccu gaauuuguau acggggacua uccaaaguau     3060

aauucauuua gagaaagaaa aucagcaaca gaaaaaguau acuucuacuc aaacauaaug     3120

aauauauuca aaaaaagcau aucacucgcu gacggcacag uaauagaacg accguuaaua     3180

gaagucaacg aagaaaccgg cgaaaguguc uggaacaaag uagcugauuu aaauacagua     3240

agaaaaguau uaagcuacag ucaaguaaau auagucaaaa aaguugaaga acagaaucau     3300

ggacuggacc gaggaaaacc aaagggacua uuuaacgcca acuuaaguuc aaaaccaaag     3360

ccaaacagua aagaaaacuu aguuggagcu aaagaauauu uagacccaaa aaaauaugga     3420

ggauacgcag guauaaguaa caguuucgca guuuuaguaa aaggcaccau ugaaaaagga     3480

gcaaaaaaaa aaaucacaaa uauacuagaa uuccaaggaa uaucaauauu agacagaaua     3540

acauacagaa aagacaaauu uaacuuucua cuagaaaaag gauauaaaga caucgacuua     3600

auaauagaac ugcccaaaua uuccuuauuc gaauuaagcg augggaguag gcgaaugcua     3660

gcgucaauuu uauccacgaa caacaaacgu ggagaaauac acaaaggcaa ucaaauauuc     3720

auaucacaaa aauacguaaa auuacuuuac caugcaaaaa gaauuagcaa uacuuucaau     3780

gacaaucaca gaaaguacgu agaaaaucau aaaaaagaau ucgaagaauu auucuauuac     3840

auacuagagu uuaaugaaaa cuacgugggc gcuaaaaaaa auggagaauu acuaaaaagc     3900

gccuuucaau cguggcagaa ccauucuauu gaugaacucu gcuccuccuu uaucggucca     3960

acaggaucug aaagaaaagg acuauuugaa cuuaccuccc gaggaggcgc agcggacuuu     4020

gacuuccuag gcguaaaaau accaagauac cgagacuaua caccuaguuc acuacuaaaa     4080

gaugcaacuc ugauccacca aucaguaaca gguuuauaug aaacccgaau agaucuagga     4140

aaacuaggug aagacuga                                                   4158


<210>  5546
<211>  4158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5546
augaaaaaua aaacuuggua ucuaggauug ggaguaggac caacaagcau aggaugggca       60

gcaacugaua gcaacuauaa acuacuacgu aaaaacaaga aacggcuaug gggaguaaga      120

cucuuugaag aagccaaaac ggcaguagau agaagaacau uccgagcaga acgacgaaga      180

cucgccagaa ggaaauggag aauaucacug cuagaagaac ucuucgcgaa agaaguaucu      240

aaaaacgacc ccaacuuuuu ccucagguua aaggaaucaa aauaccauuu cgaagauaaa      300

ucguuaauga acgaauauau acucuucaau gauaaagauu auaccgauaa agacuacuac      360

aaagaauauc caacgauaua ucaucucaga cugucacuua caacagaaga aaacccagau      420

auacgcaaag uauacuuagc uguacacucc augcuuaaaa accgaggcca uuuucuacua      480

caaggacaau cauucaauga cucgaauaua gacucuguaa uaaaagaacu acuagaacua      540

gaaacaguaa aaauaaauuu cgaagucacc gacgaaguaa uccacucacu aaagaaaauu      600

gcccuagaga agaaaaaucu aagugauaaa aauaacgacg uaaagaaacu auaccccaaa      660

gaaaaacaac uucaagaaau auucaaauug guuuuuggug gaaaaacguc acuugacaaa      720

cucuacgaca uagaagaaua caaagaacua gacaacucag ucaaaagcau auccuucaaa      780

gaaaaagucu augaagaagu aagaccggau uacgaacaaa uauugucaaa uuacaucgaa      840

cucuuggauu uagcaaaacu uguauaugau ucaauagucu uagcagacau caagaaagaa      900

ggaaaaacga ucagugaaag caaaguagaa uuauauaaca aacauaaaga cgaccuugcc      960

aauuuaaaga aauuaguaaa aauugauagu aaauuaucgg aagauaaaaa gaaagaaguu     1020

uacuucacca uuuuuaaaga agacaaagaa aaaggaacaa auuaugugaa cuacacucac     1080

cgaagccaag aaggaaaauc auguucuuau gaagauuuca agaaauuuuu aaagaaagaa     1140

cucgaaaaau uagaagauag uccaacaaaa acaacuauuc uagaagaacu agaccucgaa     1200

acguuucugc ccuuacaacg aacaaaagau aauucuguag ucccuuacca aauacauaaa     1260

gaagaacuug uaaaaauacu cgacaacgcu gcaaaauauc acaguuuccu agcugaaaaa     1320

gaugauucug gauauagcac cagagaaaaa guuguacaau uauucgaguu caggauucca     1380

uauuauauag gaccauuaaa cgcccaccac aacaccgaaa acggaggcua uucuugggcu     1440

auaagaaaga aaggaaugga auccguacca guaacaccgu ggaauuucaa agacguaauc     1500

gacgaaagcg cgagcgcaga agcauuugua aacaaucuua aaaacaaaug cgcuuaccuc     1560

ucuggagaag acgucuuacc uaaaaacucc cugcuguacu ccgaauaugc uuuauuaaac     1620

gaacuaaaug cacuaagaua ugaaggaaac cgaaucagcg uagaagccag ggauguaaua     1680

guaacaaaac ucuucaaaga acagggcaag aaaguaacaa agaaaaccau aaaagaauua     1740

cuaaaaacug aagguuuuau ugacggaaaa ggggaaauag uaggaauuga caacauaguu     1800

aaaaacgauc ugaaauccua ccgcgauuuc aagaaaauac uggcugauaa auuugacaaa     1860

gaaagaguag aaaauauaau auuguggauc acccuauacg gcgaauccag gaaacuaauu     1920

aaagagaaaa ucaccaaagu auacggagau uucuacacgg uagaugaaau agagaaaaug     1980

ucuagacuaa cauacaaaga uuggggaaua uuaucuagaa aauugcugac ugaacugguu     2040

ucagaaaaau uauauaacga agaaacuggc gaaugcuuaa auauaauagg agcaaugaga     2100

caaggaacaa uccuauucau ggaauuacuu gccgauaaau ucgacuacau gucccaaauc     2160

aaaaaucaca acaaagaaaa caaagacgau auaacugaaa ucaccccaga cauacuggaa     2220

aaucuacaug ccaguccugc cguuaaaaga ucaaucuggc aaacaguaag aauaguagaa     2280

gaacucaaaa caauuaucgg uugugucccu acaaaaaucu uuauagagac gacaaaaaga     2340

aacaacgcac ccuacaaagu acccacauug aacagaaacg acuuaguuac aaaauauaaa     2400

acaauaagag agcacgaaau uuuugaacua gaaaaagaac ucaaaaacac acuggacuuu     2460

acacuaaaca aaaaucgauu aauaaacgaa gaaccgucaa aacuuaaagc aacaaaauug     2520

uaucuauacu acacacaauu aggaagaugu auguacaccg guaagaaaau cgaucuaggg     2580

gaacuauucg acaacaacaa caaauaugau auagaccaua uauacccaca aagcaaagua     2640

aaagauggau ccuucaacaa uauaguacuc guaacaaaag cgucaaauga uaugaaagca     2700

gauguguucc cccucaaaag uaaauuucaa acaaaagaaa auaaaauauu cuggcgauuc     2760

cuuaaagaga agaaauugau aacagaagaa aaauauaaca gacuuguacg cacagaagaa     2820

uuuuccaacg gcgaacuaac uggauucauu acgagacaau uaguugaaac aagccaagcu     2880

auaaaaguca uaucaaaugu acuaagagaa cuaaauccag agacaacaau cugcuauucu     2940

aaagcggaaa acguauccuc auuuagacaa ucauuuggaa aaauaaaaga agcaaacaga     3000

aaagcggaaa acaacgaaaa gcugaucaga guucaagaaa uaaacgacua cuacaacgca     3060

aaagacgccu accuaagcau aguagucggu aacguauaug auguaaaauu uacgagaaau     3120

guccacaauu uuaucaaaaa uaacaaggac gcaagaagau acucuuuaaa uggacuuuuc     3180

uauacagacg ugaagaaugc uaacacgauu gcaugggaaa uggacaaaac cguacacguu     3240

guagagaaaa caaugaacaa uaauaacaua cuaauaacaa gacggaccag cgaacaaaaa     3300

ggagcauuau augaugcaac acuuuacaag gcuaaagucg cggaaaaagc uaaagaggga     3360

acauauuacc ccuuaaaaac aacagauagu aaaauuaaag acgucggcaa auacggcgga     3420

uuuacaaaaa uuaaaauugc cuauuauuca auauuugaau acacacuuga uggcaagaaa     3480

ggaaaacaga aaauaacaag aauaguuccu auaccgauau acauuucaca gaauauaaaa     3540

gaugacaaag cccuauugga auaugcacaa acucgacucc ccggaaaaga ccucaaaaua     3600

aaauacagaa aacuuugcau aggcucauua guuaaaauca acaacuuuaa uuacuacaua     3660

ggagguaaaa cuaauaacaa uuucacuuuc gacagcgccg uacaaguaau ccucgacaaa     3720

gacagugaau cuuacaucaa agaacucgca aaauaccuaa auuggaagaa agaaaauaaa     3780

gacgggaaac uaugggaauc aaucacagaa gauaaaaaca uacaauuaua ugauucauua     3840

gugaagaaaa ugaacaccgg cauuuuuauc cgcaaaacac cgaacaagua caccgaacuc     3900

cuaacguuag aagucaaaaa uaaauucaua aacauugaca uugacgaaca aguaaaauua     3960

uuacuagaaa uccuaaacuu auuaacgaau aagaaauuca caucuaaacu uaaugaaaua     4020

ggaaucacag cauccagagg cguauguuca uucaacuuaa guaaucaaag ucaguuuagu     4080

auaaucgaac aaucaguuac aggaguauac gaaaaagaaa agcuaauaau agagaagaag     4140

aaagaaggaa uacuuuga                                                   4158


<210>  5547
<211>  4161
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5547
augaaucaaa aauacucaau uggccuagac auaggaacua auucaguagg gugggcugua       60

auuacagacg acuacaaagu gccugcaaag aaaaugaaag uauuaggcaa caccgacaaa      120

caaagcauua agaaaaacuu gcucggagcc cugcucuuug auagcggaga gacagcagaa      180

gcuacuagac ugaaaagaac ugcuagacga cgcuacacaa gaagacguaa cagacuaagg      240

uaccuccagg aaauauucgc agaagaaaug augcaagucg acgaauccuu cuuccaacga      300

uuagacgacu cuuuccuagu agacgaagac aaaagaggag aaagacaccc gauauucggg      360

aacauagcgg cagagguuaa auaccaugau gaauuuccaa cuauuuacca uuuaagaaaa      420

caucuagcag acauaaguca aaaagcagac uuaagacucg uauaccuagc ucuagcacau      480

augaucaaau uuagaggaca cuuccuaauu gaagggcagu uaaaagcaga aaacacaaac      540

guccaagcau uauuuaaaga uuucguagaa guuuacgaua aaacaguaga agaaucacac      600

cuauccgaga uaacaguaga cgcauuaagu auucucacag aaaaaguauc aaaaucacga      660

cgacucgaaa aucuaauugc acacuaucca gcagaaaaga aaaauacauu auuuggaaac      720

uuaauagcuc uauucuuagg acuucaaccu aacuucaaaa caaacuucca auuaucugag      780

gaugcaaaac uacaauucuc uaaagauacu uaugaggaag acuuagaagg auuauuaggg      840

gaaguaggag augaauaugc ugauuuauuc gcuagugcca aaaaccucua ugacgcaaua      900

cuuuuaucug guauauuaac agucgaugau aacucuacua aagcacccuu aucagccuca      960

auggucaaaa gauaugagga acaucaaaag gaucuaaaga aauucgaaga cuuuaucaaa     1020

guaaacgccc ucgaucagua caacgcaauu uucaaagaua aaaacaagaa agguuaugca     1080

ggcuacauug aaaguggagu aaaacaagac gaguucuaua aauaccuuaa gggaauauua     1140

uuacaaauua auggaucagg ugauuuucug gauaaaauag accgagaaga uuuucucaga     1200

aaacaacgca cuuuugauaa uggaucuaua ccacaccaaa uacacuuaca agaaaugcau     1260

gccauacuuc guagacaagg agaacacuac cccuuuuuaa aagaaaacca agauaaaauc     1320

gagaaaauac ucacuuuccg uauaccauac uauguaggcc cacuugcuag gaaaggaagc     1380

agguucgcau gggcagaaua uaaggcagau gaaaagauaa cuccauggaa cuuugacgac     1440

auucuagaca aagaaaaauc agccgaaaaa uucauuacac gaaugacacu gaacgaucua     1500

uauuuaccug aagaaaaagu ccuaccaaaa cauagccuuu uguacgaaac auucacggua     1560

uauaacgaac uaacaaaagu uaaauaugua aacgaacaag gugaagcaaa auucuuugac     1620

gcuaacauga aacaagaaau auucgaucac guauuuaaag aaaacagaaa agucacuaaa     1680

gacaaauuac ugaauuaccu uaacaaagaa uuugaagagu ucagaaucgu gaaccugacc     1740

gguuuagaua aagaaaauaa ggucuucaau ucuucacugg guacuuauca ugauuuaaga     1800

aaaauacuaa acaaaucauu uuuagauaac aaagaaaacg cacaaauaau agaagauaua     1860

auucagaccc ucaccuuauu cgaagacaga gaaaugauua gacaacgcuu acaaaaauac     1920

agugacaucu uuacaaaagc gcaauuaaag aaacuugaaa gaaggcauua uacaggaugg     1980

ggaagacuca guuauaaacu cauuaacggg auuagaaaua aagaaaacaa gaaaacaaua     2040

uuagacuacc ucauagacga uggguacgcc aaccguaauu uuaugcaacu aaucaaugac     2100

gaugcguuau cguucaaaga agaaaucgcc aaagcccaaa uuauagguga cguggaugau     2160

auagcaaacg uaguacauga cuuaccugga ucgccagcaa uaaagaaagg uauccuacaa     2220

ucaguaaaaa uuguggauga auuaguaaaa gugaugggac acaauccugc gaauauaaua     2280

auagagaugg caagggaaaa ccaaacaacc gauagaggua ggagaaacuc ccaacaaaga     2340

uuaaaacuac ugcaagacuc gcugaaaaau uuagacaauc cagugaacau uaaaaacgua     2400

gaaaaccagc aauuacaaaa cgauagacua uuccuauacu acauacaaaa uggaaaagau     2460

auguacacag gcgaaaccuu agacaucaac aacuuaucac aauaugacau agaccacauc     2520

aucccccagg cuuauaucaa agacgauuca uucgacaauc gaguacucac uucaagcucg     2580

gaaaacagag gaaaaucaga uaacguccca ucaauagaag ugguuugugc cagaaaagcu     2640

gacuggaugc gcuuacgaaa agcaggcuua auuagccaac gaaaauucga uaaucuaaca     2700

aaagcugaac gaggcggcuu gaccgaaaac gacaaagcag gauucauaaa aagacagcua     2760

guagaaacga gacaaauaac uaaacacgua gcccaagugc uagaugcaag auucaaugca     2820

aagcaugaug aaaauaagaa aguaauacga gauguaaaaa ucauaacucu aaaaucaaac     2880

uuaguaucac aauuccguaa agacuucaaa uucuauaaag uacgugagau caacgacuac     2940

caucacgccc augaugccua ccuaaaugca guaaucggga ccgcacuccu aaagaaauau     3000

cccaaacucg caagugaguu cgucuauggc gaguucaaga aauaugaugu acgaaaauuu     3060

auagcaaaaa gugacaaaga aauaggaaaa gcaacugcaa aauauuucuu uuauucuaac     3120

cugaugaacu uuuucaagaa agaagucaaa uucgcagacg ggacuguagu ugaacgaccc     3180

gacauagaaa caagugaaga uggcgaaaua gcuuggaaca aacaaacuga cuuuaaaaua     3240

guucgcaaag uauugucuua cccucaaguc aacauuguua agaaaacaga aguccaaacu     3300

cacgguuuag auagaggaaa accaagggga cuauucaaug cgaacccauc accuaaacca     3360

aaaccggaua gcucagaaaa ucuagucggc guaaaaagaa acuuagaccc gaagaaauac     3420

gguggauaug ccggaaucag caacucauau gcaguauuag uaaaagcaau uauagaaaaa     3480

ggagugaaaa agaaagaaac aaugguuuua gaguuccaag gcauauccau acucgauaga     3540

auaacguuug aaaaagacaa acgcgccuuc uuauuaggaa aaggauauaa agacauuaag     3600

aaaauaaucg aauuacccaa auacucacua uucgaauuga aagaugguuc acgaaggaug     3660

uuagcaucga uacucuccac aaauaauaaa agaggcgaaa uacacaaagg aaacgaacuu     3720

uuuguacccc aaaaauucac aacucucuua uaucacgcaa agagaaucaa uaaccccauc     3780

aacaaagauc auauagaaua uguuaagaaa cauagagacg acuucaaaga auuauuaaac     3840

uacgugcucg aauuuaacga gaaauaugua ggagcuacga aaaauggcga aagacuaaaa     3900

gaagccguug ccgauuuuga uuccaaauca aaugaagaga ucuguacuuc auuuuuagga     3960

gcaguaaaca guaaaaaugc aggucuguuu gaauuaacca gccuaggaag ugcauccgac     4020

uuugaauuuc uaggcgucaa gauaccaaga uacagagauu acacaccauc gagccugcuc     4080

aaggacucca cccucauaca ccaaaguaua acggguuuau acgaaacaag aauagaccua     4140

aguaaauuag gagaagacug a                                               4161


<210>  5548
<211>  4161
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5548
augacuaaaa uaacagaaga cuacauaguu gguuuggaca ucggcacaaa cucaugugga       60

uggguagcaa uggauagcaa aaacacaauu uuaagaaugc acggaaaaac ugcaauuggu      120

ucacaucuau ucgacgcugg aaacagugca gcugauagac gcgcguuucg cacaacucga      180

agaagaauaa aacgccguaa auggagauua aaauuacuug aagaaauuuu ugacucgcac      240

cuagccaaag uagauccgua uuuuuucgcu agacuuaaag agagcggacu cucacccuua      300

gauacucgaa aaaacguuuc aucaaucauc uaccccacuu cgcuagaaga caaaaaauuu      360

uacguagaua acccaacaau auaccaucua agauacaaac ucaugacaga agaccguaag      420

uucgaucucc gagaaguuua ccuugccauu caccacaucg ugaaauaccg aggaaacuuc      480

uuguacaaca cuccaguuaa agcauucgaa gcaucaaaaa uagauguaaa agggucacua      540

aaaaaacuaa augaacugua cgagggcuua gacucugaau ucaacgucga acuugacagc      600

aacaacgccc uggaaauaga gaauauaaua cgcgacaaaa aaaucuucaa aauuaacaag      660

guaaaagaaa uaaaccaacu ucucagcuua aaaacagaag auaaagaaag gacaaaacuu      720

augaaagaug ucucaaaaca aaucguaaac gccaucuuag gauacaaaac caaguucgaa      780

accauuuuac uaaaagacgu caguaaagac gaagcggaug auugggaauu uaaacuaacc      840

gacguagacg cugacaacaa auucgagaac cucauaggag acuuaaauga gaacgaacaa      900

gaaauacucc uaguaauucg caaacuagcu aacgcaauaa cacuaagcaa cauaguugaa      960

gaaggaaaaa cauuaagcga aucaaugaaa gauaaauaua aaaaacauuc ggaugaccuu     1020

aaguugcuaa aacagguuau uucggaucac ccggacaggg auaaagcaaa aaaauuggca     1080

cuugcauacg aucucuacgu uaacaaucga cacggaaaaa uucuacaagc gaaagauauc     1140

uuagaaaaaa agaaaacccu uagcaaagaa gauuucuaua aagaaauaaa aaaaaaccua     1200

gacgacucua aagaaucuca agaaauauua gacgcuauau cucuagauuc uuucaugcca     1260

aaacaaagaa cuaacgaaaa uggagucaua cccuaucaau uacaccaauu agaacuagac     1320

aagauaaucg aaaaccaagg caaauacuac ccauuuuuaa aagaaguaaa cccagucucu     1380

ucccauuuaa aacaagcucc cuauaaacua gaugaauuaa uaagguuucg aguucccuac     1440

uacgucggac cacuaauauc uccuaaugaa ucuacaaaaa auaaacaaac caagaaaaau     1500

caaaauuuug ccuggaugau aagaaaagaa cagggccaaa uuacgccgug gaacuuugau     1560

caaaagguag aucgaauggc uucagccaau aaauucauca aaaggaugac aacgaucgac     1620

acauaucuac ugggagaaga cguauugcca gcuaacagcc ucuuauauca aaaauucaca     1680

guacuaaacg aacuaaacaa cguaaaaaua aauggcaaga ggauaagugu cccacucaaa     1740

caagaacugu acaauaacuu auucaagaaa aauucaacag uaacaacaaa uaaacuaaag     1800

ucauaucuua aagaaaauua caagcuaauu aaaguagaaa uaaaagguuu aagcgacgaa     1860

aaaaaauuca acucuggacu uaccacauau aacaaauuaa aaaaucuaaa aauuuucgac     1920

caacaaauag acgaccccaa uuacaacaaa gacuuugaac guauuauuga auggucuacc     1980

auauucgaag acaaggcgau auacaaagaa aaauuaaaga ccauagacug guuaucagac     2040

agacagauag auaaauuaag caaaauuagg augcaagguu ggggccaacu cucaaaaaaa     2100

cuacuaucac aacuaacaga caauaacgga caaacuauua uagaacaacu cugggacucc     2160

caaaacaacu uuaugcaaau aguaaaucaa gccgauuuua aggacgccau cgcaguugca     2220

aaccaaaacc uauuaguaga cacuucagua gaagacauac uaaacgaggc cuacacuucu     2280

ccagccaaca aaaaagcaau caggcaagug guaaaaguug uugacgauau cguuaaagca     2340

acaagcggaa aaguaccgaa acaaauagca auagaauuua ccagagaugc ugaugacaac     2400

gcaaaaauau cacaaagccg cgcaaacaaa cuacgcaaag uauacaaaga gaugucuaau     2460

gaauuagcga gugaaacuau aagaaacgaa cuagaaaggg uagcaaaaga ucaaaaacuc     2520

cuaaaagaua aacacuacuu auacuuuaug caauuaggga gagacgcuua cacuggagaa     2580

uuaauagaua uagacaaccu cgaacaauau gauauugacc acguacuacc acaaucuuuc     2640

auaaaggaug acucucuuga aaacagagua cuaguaaaaa aagcugucaa uaacggcaag     2700

agcgacaaug ucccagucaa acuauacgga aauagaaugg cgacagauuu aggaaugacu     2760

auacgucaca ugugggaaaa guggaaagaa caaggucuaa uaacaaaaac uaaauacaac     2820

aaccuaauaa uagaccccga uaaaaucaac aaauaugaaa gcaguggcuu uauacaccga     2880

caauuagucg aaacauccca aauuauaaaa uugguuucga ccauccuuca aucuaaauac     2940

cccgauacag aaauaaucgu cgucaaagca agauauaauc acuaucuaag aaaaagauua     3000

aauuuauaca aauccagaga aguaaacgau uaccaucaug ccauagaugc cuacuuauca     3060

gcaauaugcg gcaaucuacu auaucaagua uacccauacc uccgaccauu cuucguauac     3120

ggacaauaca aaaaauuuuc aucagagccu gaaaaagaaa aaauuaucua ugacaaaaca     3180

agaagauaua acuucauauc gcagauauuc gaaaacaaag auaaugacau aauauccaga     3240

gaaaccaaaa aaaaggucuu ugacaaaaaa gacauuauug aaaaacucaa acgagcuuau     3300

aauuacaaau acaugcuagu aagucgagaa accgagacac acgaccaaga aauguucaaa     3360

augacaguuu acccaaggcu cagccgcgac acaaaaaaau caagaaaucu aauaccaaaa     3420

aaaaaagaua ugccaaccga gaucuaugga ggauauacua acaauucuga ugcauauaug     3480

aucauagcua gaaucaauaa aaagaaagga ccagaauaua gaguuuucgg uguacccaug     3540

agagaacuag uaaauuuaag aaaggcagaa aaaaacggac acuauaaagc uuaccucaaa     3600

cagguauuag aaccagagau aauguacucg aaaaacggaa agaaaaauaa aacuauaagc     3660

agcuucgaaa uuguaaaagc uaaaguacca uacaaacaag ucauacuaga uggagacaag     3720

aaauuuaugc uagcaucuuc aacuuauguc caaaaugcca aacagcuaac ucuuucacaa     3780

uacaguaugc aagcaauaac agauaauugu gaaaacgaca cugaagaaga aaaagcauug     3840

guagaagccu acgacgaaau auuaacaaac auagauaaau accuaccguu auuugauaua     3900

aacaaauuca gagaaaagcu acacgcaggg agagaaaaau uuguagaucu accauuagcu     3960

gaaaaaaaag agaccauauu acaagugcua aacgguuuac acgacaaugc aguaaugccu     4020

aagauaaaau cacuaggacu aucaaccccu cuagguuuca ugcaauucca cacuggcguc     4080

ucauuaagug agaacgcaaa acuaauauau caaagcccca ccgguuuguu cgagaagaga     4140

uuaaaaauuu cugaucucug a                                               4161


<210>  5549
<211>  4164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5549
augucggauu ccaaaaaauu cgaaggcgaa aauguaaaau ccggcgauaa aaaaacuaac       60

aaaaaccaug guaaaguccu aaaaucaagu ccuaaagaaa ucagaaaaaa cacagaauau      120

uuuauaggac uugacauagg cacgucauca guuggcuggg cugucaccaa cacagacuac      180

gaaauacuaa aauuuaaugg caagagaaug uggggaagua gguuauucaa ugaagcauca      240

acagcagagg aaagaagagg ucaccgagua aaccgcagaa gaauacaaag aagacgagau      300

agacuaaacc uguuagaaga gaucuuuagu gaagaaaugg caaaagugga uccugaauuc      360

uuuaugagac uuaaagaauc aaaguuucac uuggaagaca aagaugaaag aauacuagga      420

gagucaaaua uaauauuuaa aggaagcgaa gacggagaua agaaauacua cgaaaaauac      480

ccaaccauuu aucaucuacg acauaaauua augcaaaguu gcgaagaugu agacauaagg      540

gaauuauaua uggcauuaca ucacaucaua aaauacaggg gccacuuucu auucaaagga      600

gauuuugaag aaaccggcac uagcauagaa aacauaauaa uugaauuacu agaacuaauu      660

aauaauaacc uaggaguaaa aaaauucgaa uauaacgaaa gucuaauuaa ugaauugaaa      720

aacauaguga guaauacgag caacaccaua accaacaaaa aaaagcuacu cggcgaguuu      780

uucaacaaaa aagaauacaa agcacacaag aguuuauuca ucuuaucaau cggagccagu      840

gugaaacccg uagacuuauu ucuagaggaa gaauacaaag acauagauga uauaaaaaau      900

aucaguuuca agaauuuaga auacgaucaa guacgcgaua ccuaugaauc gauuuuuuua      960

gacaaaauag aauucaucga cguagccaaa agauuauacg acgcuacgau ucuccuguca     1020

auaaaaccag aaggaaaaac auuaagugaa gcuaaaguag acauauauaa caaacaccac     1080

aacgacuuag aacuacugaa gaaagucuua aaaacagaca aagagaaaua ugacucuauc     1140

cuaaagcuag auaucaagaa agguaacaau uacguaaacu acgucaaaaa aaguaaaaac     1200

gguauaucau guaguaaaga agaauuucac aacgcucuca agaaaguacu aguuaaaaua     1260

gaaaaaacag augacauaaa auauauacuu aaugaaauag aacuggaacg cuuccuacca     1320

uuacaaagag ucaaagacaa cagugucauc cccuaccaaa uccacagaua ugaguugaaa     1380

aaaauucuug agaaugcaag caaaaaguuu acuuuuuuaa augaagaauc agauggccac     1440

acaaacaucg agaagauaau aaaaauaaug gaauaccgca uaccauacua uguuggcccc     1500

uugaaugauc accacagcgu auauaacaau gguuuugcau ggguaagcag aaaaagaagu     1560

ggaagaauca cccccuggaa cuucgacgaa augguugaca aagaagaauc agcagaaaga     1620

uuuauagaaa auuuaacaaa uaaaugcucg uacuuaauag gagaagaugu ucuacccaaa     1680

gaaucauuac uuuacucaga auucucauua uuaaaugaac uaaacaauau aaaauaugac     1740

ggcauaucau uacccaucga uauaaaaaaa ucaauagugg aaaagauauu cagaguagaa     1800

aaaaaacgag uaacgaaaaa auacauuaaa gaauuucuaa aacaagaagg auauucugac     1860

gguaaaggag aaguaagugg cauugauauc gauauaaagu cugauuuaaa aucauacaga     1920

gauaugguga aaauuuucgg uaccgaaaua gacuauagaa uggcugaaaa uaucauaaaa     1980

uggguaacgc uuuucggaga cagcaaagac cugcugaaaa ccaaaauaaa caaagaauau     2040

ggagaaaaag uauccaaaga ucaacucaaa aaaguaugua aguuaaaaua cagggauugg     2100

ggacgacucu caaaaaaauu ccuaaccggc auucgaucaa gcguacucgu aaacgaugcu     2160

acaggcgaag gauacaccau aauugaggca augagaaaua cagauaauaa cuuaaugcaa     2220

cuacuaagaa acgaauacga cuaccuaaag aaaaucgauc aaauaaauau gcucagcaga     2280

aaagacauag augaaaucag ucaugacaua uuagaugacc uauacugcuc accagcugug     2340

aaacgaucac uauggcaaac aaucaaaaua guagaagaaa uaaaaaagau aaugggccau     2400

gaacccaaaa aagucuuugu agaaguaacg agaacuaaca aagcugauaa aaaaacaaca     2460

aucacgagaa agaaucgccu ucuagaacuu uacucagcca uaaaugcaga aagcagagau     2520

ugggucaacg aacuuaauuc aacugaagug ucaaaauuua acucuaaaaa acucuaccuu     2580

uauuacacuc aaauggguaa augcauguau acugggaaaa acauugacau agaaucauua     2640

uuuacagaua uguaugacau agaccacauc uuuccucgaa guaaaacaaa agauaauagu     2700

uucgaaaauu uaguacucgu aacacguaaa gccaacgcgg acaaaagaga cguuuaucca     2760

auagacccaa aaauccaaac cgaaagaaaa agucuauggg auauguuaca ugauaaaaaa     2820

cuaauugggg auaaaaaaua ugaccguuua acucgaacaa cugguuuuac ggacgacgaa     2880

augucuggau ucauagcacg gcaauuaguu gagacuucac aagcaucaaa agcagcggcg     2940

gaaauguuaa aacuaauuaa caacgacagc gaaauaguau acguaaaagc agaaaacguc     3000

ucagauuuua gacaugaaaa cgaauucaua aaagucagag aaauaaauga ccaccaccau     3060

gccaaagacg cauaccuaaa uauaguugua gguaauguuu auaacgaaaa auucacaaag     3120

aaccccgcca auuacauuaa gaaggguggc ggaaauaauu auucccucaa uugcauauuc     3180

aaccgaaacu uagaucguaa cggcaaaaga auaugggacg aaaaaaaauc aauagacaca     3240

gugaacagaa ugaugaaauc aaacgacguc cgaguuacau uuaaaaauag aguagauaaa     3300

ggaccgauac uuaauaaagu gacaauccac aaggcaagcg aauucaugga cgacgguaaa     3360

aaaaauaaaa aaggauuccc aauaaagucc ucugagaaaa acggcaauaa cagacucugu     3420

aauuuauaca aauauggagg uuacaaagau uuaaacuacu cucauuacac cauaguacga     3480

uacaacguau augaaaaaaa aggauuagac aucaaauacu acaugauacc aauuccauau     3540

uaucucugcc uaugcaauau aaaccaacau gacuacguau acgacuauau aaguaacaga     3600

ucuaaaaaag acgucaauaa uauagaaauc auaguaaacg aauuaaagau gggcucgcua     3660

aucaauaucg acggauacaa cuacuaccua ggaggaucaa aaccaaaaac uuuuuauguc     3720

gacccagcau aucaaauaau acuagacaaa gaaauagaaa ggugcauuaa agagauagua     3780

aaagccaaga acaaugagua ucgauuuaua aauacugaua aaauuaacaa aaacuuaaac     3840

augaaaauau acaaauacau cguaaacaag aaaauuauug gaguuuuuag aaacagaaaa     3900

ugggauaaag gggaauaccu aaauuccuca gaaacaauag aaaaauuuuc aggccucucu     3960

gucgaaaguc aauguaacgu cauacuaaga uuaauaaaug auaugagggg aagcaacaaa     4020

ggaggugcaa acagcaaauu cgacgaauua gaaaugacaa uaagcagggc agaaaucccc     4080

caaacacuca guaauguauc auccuucaag aucauaaacc aaaguauaac uggucuauuu     4140

gaaaaugaag ucaccauagu auga                                            4164


<210>  5550
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5550
augacaaaac cauauaguau aggccuggac auaggaacua auucaguagg augggcaguc       60

auaaccgacg auuauaaagu uccaucgaaa aaaaugaaag uacucggaaa uaccucgaaa      120

aaauauauaa aaaaaaaucu auuaggcgcu uuacuuuucg acucuggaau aacugcagaa      180

ggacgcagau uaaagagaac agcuaggaga agguacacca gaagaaggaa ucgaauacug      240

uacuuacaag aaauauuuag uaccgaaaug gcgacauuag augaugcauu cuuucaacgc      300

cucgaugacu cauuuuuggu uccagaugac aaaagagaua gcaaauaucc gaucuuugga      360

aaucuaguag aagagaaagc auaccacgac gaauucccaa ccaucuacca ucuacggaaa      420

uacuuagcag auuccacuaa aaaagcugau cugcguuuag uuuacuuagc auuagcccac      480

augauuaaau accgaggcca uuucuugaua gaaggcgagu uuaacaguaa aaauaaugac      540

auacagaaaa acuuucaaga uuuccuugac acauacaaug caauuuucga aucagaucug      600

ucguuagaaa auucuaaaca auuagaagaa aucgugaaag acaaaauauc uaaauuagag      660

aaaaaagacc gaauacucaa acuauuucca ggugagaaaa auagcggcau cuucucagaa      720

uuccuaaaac uuauaguagg caaccaagca gauuucaaaa aauauuucaa ucuagacgaa      780

aaagcaucau uacacuucag uaaagaaagu uaugaugaag aucuagagac ccuacuugga      840

uauauaggcg augauuacag ugauguuuuc cuaaaagcca aaaaacucua cgacgcgauc      900

uuauuaucag gaauccuuac aguaacagau aacggaacug aagcucccuu auccagcgcg      960

augauuauga gauauaaaga acacgaggaa gaccuggggu uauuaaaagc cuauauaagg     1020

aacauaucuc ucgaaacgua caaugaagua uuuaaggaca auaguaaaaa ugguuacgca     1080

ggcuauauag acggaaaaac uaaccaagaa gacuucuacg uauaccuuaa aaaucuguua     1140

ucaaacuuug aaggggcuga uuacuucuua gagaagauag auagagaaga uuuucugcgc     1200

aaacaaagga cauucgacaa cggcucuauu ccuuaccaaa uccaccuaca agaaaugagg     1260

gcaaucauag acaagcaagc uaaauuuuac ccauucuuag cuaacaauaa agaaaggaua     1320

gaaaagauac uaacuuuccg aauaccuuau uauguuggac cucuagccag aggaaauagc     1380

gacuucgcau ggaguauaag aaaacgaaac gaaaaaauaa cuccauggaa uuuugaagac     1440

guaauagaua aagaaaguag cgcagaggca uucauuaaca gaaugacauc uuuugauuua     1500

uauuuacccg aagaaaaggu ucuaccaaaa cauucccugc uauaugaaac auuuaacguc     1560

uauaaugaac ucacaaaagu acgcuuuaua gcagaaucua ugagcaaaua ucaauuccua     1620

gacagcaaac aaaagaaaga cauaguaagg cuauacuuua aggacaaaag aaaagugaca     1680

gacaaggaua uaauagaaua uuuacacgca aucgacggau acgauggaau agaauugaaa     1740

ggaaucgaaa aacaauucaa cucaucccuu ucaacauauc augaccuacu aaauaucauc     1800

aaugacaaag aauuccucga cgauuccuca aacgaagcca uaauagaaga aauaauacac     1860

acacucacaa ucuucgaaga cagagaaaug auaaaacaac gccuaucaaa auucgaaaau     1920

aucuuugaca aaucaguucu gaaaaaacuc ucacgaaggc acuauacagg auggggaaaa     1980

cuaagcgcaa aauuaaucaa cggaauaaga gacgaaaaau cagguaacac uauucuagac     2040

uaucucauag acgauggaau uaguaaucgu aauuucaugc aauuaauaca ugacgaugca     2100

uuaucauuua aaaaaaaaau ccaaaaagca caaauaauag gagacgaaga uaaggggaau     2160

auaaaagaag uagugaaguc ucuaccaggc aguccagcaa uaaaaaaagg gauccuccaa     2220

agcaucaaaa uagucgauga auuagucaaa gucaugggag gucgaaaacc agaguccauu     2280

guaguagaaa uggcccgaga aaaucaauac accaaccaag guaaaucaaa cagucaacaa     2340

agauuaaaaa gacuagaaaa aucgcuuaaa gaacucgggu cuaaaauccu aaaagaaaau     2400

auaccagcaa aacuuaguaa aauagauaau aaugcccuac aaaacgauag acuuuaccuu     2460

uacuaccuuc aaaacgguaa agauauguac acgggagaug accucgacau cgaccguuua     2520

uccaauuaug acauagacca cauaaucccc caggcauucu uaaaagauaa uagcauagac     2580

aacaaaguuu uagucaguuc agccucuaac cgaggcaaau ccgaugacgu cccaagcuua     2640

gaaguaguaa aaaaacgaaa aacucucugg uaccaacuac uuaaaagcaa acuaaucagu     2700

caacgcaaau ucgauaacuu aacaaaagcu gaacgaggag gacuaagucg agaagacaaa     2760

gcgucauuca uccaaagaca acugguagaa acccgccaaa uaaccaaaca cguugcacga     2820

cuauuagacg aaaaauuuaa caauaaaaaa gaugaaaaua accgggcagu aagaacagua     2880

aaaaucauua cauuaaaaag uacucuagug agucaauuua gaaaagauuu ugaguuauac     2940

aaaguaaggg aaaucaacga ccuacaucac gcgcacgaug ccuaccuuaa ugcaguggua     3000

gcuucagcac uacuaaagaa auacccaaaa cuagaacccg aauuuguaua uggcgauuac     3060

ccaaaauaua auucauuuag agaacgcaaa ucagccacug agaaaguuua uuucuacagc     3120

aacaucauga acauauuuaa aaaauccauc agcuuagcug acgguaccgu aaucgaaaaa     3180

ccuuuaauug agguaaacga agagacagga gaaucaguau ggaauaaaga gucagaucuc     3240

gcuacgguaa gaaggguccu uucauauccc caaguaaacg uaguaaaaaa aguagaaaaa     3300

caaaaccacg gacuugauag aggaaagcca aaaggcuuau ucaacgcaaa ucucaguuca     3360

aaaccgaaac cgaauucuaa ugaaaaccua guuggugcca aagaauaccu agauccgaaa     3420

aaauacggug guuacgcagg uauaucaaac ucauucgccg uacuaguaaa aggaacaaua     3480

gaaaaaggag caaaaaaaaa aauuacaaac guacuggaau uucaaggaau aagcauuuua     3540

gacagaauua auuacaggaa agacaaauua aacuuccucu uagaaaaagg cuacaaagau     3600

auagaauuga uaauugaauu accaaaauac ucacuuuuug aauuaucuga ugguuccaga     3660

cgaaugcuag cuuccauauu auccaccaac aacaaaaggg gagaaauaca caaaggcaau     3720

caaauauuuc uaucacaaaa auucgucaaa cuucuauacc acgcuaaaag aauaucaaac     3780

acaauaaacg aaaaccauag aaaauauguu gaaaaccaca aaaaagaauu cgaggaacua     3840

uucuauuaua ucuuagaauu uaacgaaaau uauguugggg caaaaaaaaa cggcaaacug     3900

uuaaauucag ccuuccagag uuggcaaaac cauucuauag augaauuaug cuccucauuu     3960

auagggccaa ccggaagcga aagaaaaggc cuuuuugaau ugacaucucg agggucagcg     4020

gccgacuuug aauuuuuagg gguaaaaaua ccaagauacc gagacuacac uccaucauca     4080

uuacugaaag augcuacccu cauacaucaa aguguaacgg gacuauacga aacuagaaua     4140

gaucuagcaa aauuaggaga agguuga                                         4167


<210>  5551
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5551
augacaaaac cauacuccau cggccucgac auaggaacga acucuguagg uugggcaguc       60

auaacugaca acuacaaggu gccuucaaaa aagaugaaag uucuagguaa caccucaaaa      120

aaguauauaa aaaaaaauuu acuaggagua uuauuauucg auaguggcau aacagccgaa      180

ggaagaaggc uaaagaggac agcaagacga agauacacac gacgaagaaa uaggauccug      240

uauuuacaag aaauuuuuuc cacggaaaug gcaaccuuag augacgcuuu uuuccaacgc      300

cuagaugacu cauucuuagu gccagacgau aaacgagauu cuaaauaucc uauauucgga      360

aauuuaguag aagaaaaagu uuaccacgau gaauucccaa caauauauca ccuacgaaag      420

uauuuagcag acucaaccaa aaaagcggac cuacgccuag uuuacuuagc acuagcacau      480

augauuaaau auagaggcca uuuuuugaua gaaggagaau ucaauuccaa aaauaacgau      540

auacaaaaga acuuucaaga uuuucucgac acuuauaacg ccauauucga aagugauuua      600

ucacuagaaa acucaaaaca acuugaagaa auugugaaag acaaaauuuc aaaacucgaa      660

aagaaagaca gaauccucaa acuuuuccca ggagaaaaaa auuccgguau cuuuucggaa      720

uuccuaaaac ugauaguagg aaaucaggcu gacuucagga aaugcuuuaa ccuagacgaa      780

aaagcaucau uacacuuuuc caaggaaucu uaugacgaag aucucgagac acuauuagga      840

uauauaggag acgacuacuc agacgucuuc cuaaaagcaa aaaaauuaua ugacgcaaua      900

cuuuuaucug gauuucuaac agucacggac aaugaaaccg aagcgccauu gagcagcgca      960

augauaaaaa gauacaacga acacaaagaa gacuuagcac uauuaaaaga auauauacga     1020

aacauaucau uaaaaaccua uaacgagguu uucaaagaug auacaaaaaa ugguuacgcg     1080

gguuauauug acgguaaaac gaaucaagaa gacuuuuaug uuuaucuaaa aaaccuacuu     1140

gcugaauucg agggagcaga cuauuucuua gaaaaaauag accgcgaaga uuuucucaga     1200

aaacaacgca ccuucgauaa ugguaguaua cccuaucaga uacauuuaca agagaugaga     1260

gcaauucuag acaaacaagc aaaauucuau ccauucuuag ccaaaaauaa agaaagaaua     1320

gaaaaaauau ugaccuuuag aaucccauau uaugucggac cucuagcaag aggaaauuca     1380

gauuuugcau ggucaauaag aaaacguaau gaaaaaauaa caccauggaa cuucgaagac     1440

gugauagaca aagaauccag cgcagaagca uucauaaaua gaaugaccuc auuugaucug     1500

uauuuacccg aagaaaaagu acuaccaaaa cauucauuac ucuacgaaac cuuuaaugug     1560

uauaacgaac ugacaaaagu aagauuuaua gcggaaucaa ugagagauua ccaauuucua     1620

gacagcaaac aaaaaaaaga uauaguucga uuauacuuca aagacaaaag aaaaguaacc     1680

gauaaggaua uaauugaaua ucuacaugca aucuacgguu acgacggaau agagcucaaa     1740

ggaauagaaa aacaauucaa uaguucauua ucuacguauc augaccugcu aaauauaaua     1800

aaugauaaag aauucuuaga cgacucuuca aaugaagcua uaauugaaga aauaauacau     1860

acacuaacga ucuucgaaga ccgugaaaug auaaaacaaa gacuaaguaa auuugaaaau     1920

auauuugaua aaucaguacu gaaaaaacua uccaggagac auuacacagg augggguaaa     1980

uuauccgcca aauuaauaaa cggaauucgu gacgaaaaau cugguaauac aauccuagac     2040

uaccuaauag augauggcau auccaauaga aauuucaugc aacugauaca cgacgaugca     2100

uuaaguuuca aaaaaaagau ccaaaaggcg caaauaauag gugacgaaga caaagggaac     2160

auuaaagaag uaguaaaauc auuaccagga ucacccgcua uaaaaaaagg cauauugcaa     2220

ucuauuaaaa uuguugauga acuuguaaaa gucaugggag gaagaaaacc agaaaguaua     2280

gugguagaga uggcacgcga aaaccaauau accaaccaag gaaaaucaaa cagccaacaa     2340

cgacuaaaac gacuugaaaa aagccuuaaa gaauuggguu caaaaauacu caaagaaaau     2400

aucccagcaa aacuuucgaa aauugacaac aaugcauugc aaaacgaccg auuauaccuc     2460

uacuaccuac aaaacgguaa agauauguac acaggagacg accucgacau ugacagauug     2520

ucgaacuaug acaucgacca uauaauacca caggcauucc uuaaggauaa cucaauagac     2580

aacaaaguac ucguaaguuc agcgaguaac cgaggaaaau cggaugacuu uccaucucuc     2640

gagguaguca aaaaaagaaa aacauucugg uaccaacuac uuaaaucuaa auuaauaucu     2700

caacgcaaau uugacaaucu cacaaaagca gagcgcggcg gucuauuacc ugaggacaaa     2760

gcaggcuuua uacaaagaca acucguugaa accagacaaa uaacaaaaca cgucgcaaga     2820

cugcuagacg aaaaauucaa uaauaaaaag gaugaaaaua aucgugcugu acgaacagua     2880

aaaauaauaa cauuaaaauc caccuuaguu ucacaguuua gaaaggauuu ugaacuauac     2940

aaagucagag agaucaacga cuuucaucac gcucaugaug cuuacuuaaa cgccguuaua     3000

gcaagcgcuc uacucaaaaa guauccaaaa cuugaaccug aauuugugua cggugacuac     3060

ccaaaauaca auucauuuag agaacgcaaa ucagcuacag aaaaaguuua cuuuuacagc     3120

aacauaauga auauuuuuaa aaaaucuaua agucuagcgg auggcagagu aauagaaaga     3180

cccuuaauag aaguaaacga agaaaccgga gaaucaguau ggaacaaaga gucagaucuu     3240

gccacaguaa gaagaguauu aaguuaccca caagucaaug uaguaaaaaa aguagaagaa     3300

caaaaucaug gguuagaucg uggcaaaccc aaaggacuau uuaacgcaaa ccuuucaagu     3360

aaaccaaaac cuaacucaaa cgaaaaucuu guaggugcua aagaauacuu ggauccaaaa     3420

aaauacggug gauacgcagg aauaucaaac ucauucgcug uauuaguaaa agggaccauu     3480

gagaagggag caaaaaaaaa aauaaccaac gucuuagaau uucaaggcau aucaauauua     3540

gaucggauca auuacagaaa agacaaauua aauuuuuuac uggagaaagg auauaaagac     3600

aucgaauuga uaaucgaacu accaaaauau uccuuguucg aauuaucgga uggaucaaga     3660

cgcaugcuag cgucuauacu aucgacaaac aauaaaaggg gagagaucca caaaggcaau     3720

caaauauuuc ucucacaaaa auucgucaaa cuccucuauc acgcaaaaag aaucucuaau     3780

acaauaaacg aaaaccaucg aaaguacgua gaaaaccaca aaaaagaauu ugaagaacua     3840

uucuacuaua uacucgaauu caaugaaaau uauguaggug caaaaaaaaa cggcaaauua     3900

cuaaacucag cauuucaauc auggcaaaac cauuccauag acgaacugug caguucauuc     3960

auagguccaa ccggaucaga acgaaaggga cucuucgaau uaacaucgcg cggcagcgcc     4020

gccgauuuug aauuuuuagg aguuaaaaua ccacgauauc gcgacuacac gccuucaagc     4080

uuauugaagg acgccacguu aauccaucag agugucacug gccuauacga aacgcgcaua     4140

gaucuagcaa aacuaggaga aggauga                                         4167


<210>  5552
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5552
augacaaaac cauauucuau aggucuagau auagguacaa auuccguagg gugggcugua       60

aucacagaca auuacaaagu acccagcaag aaaaugaaag uacuaggcaa cacuuccaag      120

aaauacauca agaaaaaccu uuuaggcgug cuacuauuug acuccggaau aacagcugaa      180

gggcgaagac uaaaaagaac agcacgaagg cguuauacaa gacgucgcaa ucgcaucuua      240

uaucuccaag agauauucag caccgaaaug gcaacccucg augaugccuu cuuccaacga      300

cuagaugauu cauuccucgu accagaugau aaaagagacu ccaaauaccc cauauucggu      360

aauuuaguag aagaaaaagc auaucaugac gaguucccua ccaucuauca ucuccgaaaa      420

uacuuagcug acucuaccaa gaaagcagau uuaaggcucg uauaucuagc cuuagcccac      480

augauaaaau accgaggcca uuuccuaaua gaaggagaau uuaacaguaa aaauaacgac      540

auucagaaaa acuuucaaga uuuccucgac acauacaacg cuaucuuuga aucagacuua      600

ucauuagaaa acucaaaaca acucgaagaa auaguaaaag auaaaaucuc caaacuagaa      660

aagaaagaua ggauacuuaa acuauuccca ggagagaaaa acagugguau uuucucagag      720

uuccuaaaau uaauaguagg aaaucaagca gauuuccgaa aaugcuuuaa cuuagacgaa      780

aaagccaguc uacacuuuag caaagaaagu uacgacgaag acuuagaaac auuacucgga      840

uauaucggag acgauuacuc cgacguauuc auuaaagcua agaaauuaua cgaugcgauu      900

cuauuaucgg gcuuucuaac uguuacugac aacgaaacag aagcaccauu aucaucggcg      960

augauaaaac gauacaacga acacaaagaa gaccuugcac uucuaaaaga auauauacgu     1020

aauauuucau uaaaaacaua caaugaagua uucaaagaug auacgaaaaa cggguaugcc     1080

gguuacauug acggcaaaac aaaucaagaa gauuuuuaug uauaccucaa aaacuuacua     1140

gcagaguucg aaggggcgga uuauuucuua gagaaaauag acagagaaga uuuccuaagg     1200

aaacaaagaa cauuugauaa uggaucaaua cccuaccaaa uccaucuaca agaaaugaga     1260

gcaauccuag auaaacaagc aaaauucuac ccauuucuag cgaaaaacaa agaaagaauc     1320

gagaaaauac uaacauuuag gauaccauau uauguagguc cauuagcaag aggcaauagc     1380

gacuuugcau ggucuauaag aaaaaggaac gagaaaauaa ccccuuggaa uuucgaagac     1440

gucauugaca aagaauccag cgcggaggcu uuuauuaauc gaaugaccag uuucgacuua     1500

uacuuacccg aagaaaaagu gcuuccaaaa cacucacucc uauacgaaac cuuuaaugua     1560

uauaacgaac uaaccaaagu aagauucaua gccgaaagca ugcgagauua ccaauuucuu     1620

gacucaaaac agaagaaaga uauuguuaga cuauauuuca aagauaaacg gaaagugacc     1680

gacaaagaua uaaucgaaua ccuacaugcu auuuacggau acgacgguau ugaauuaaaa     1740

gggauagaaa aacaauucaa uucaagucuc ucaacauauc acgacuuacu caacauaauc     1800

aaugacaaag aguuccugga ugauucaagc aacgaagcca uuauugaaga aauaauacac     1860

acacuaacaa ucuuugaaga ucgagaaaug auaaaacaac gcuuaaguaa auucgaaaau     1920

auuuuugaca aaagcguacu caagaaacuu ucucgacgcc acuacaccgg auggggaaaa     1980

cuaucugcaa aauugaucaa cgggauacgu gacgaaaaau cggguaauac uauucuagac     2040

uaucucauag acgauggaau cuccaaccgc aacuuuaugc aacuaauaca cgaugacgca     2100

cuuucauuua aaaagaaaau ucaaaaagcg cagauaauug gagaugaaga caaaggcaac     2160

auaaaagaag uaguaaaauc ccuaccggga uccccagcua ucaagaaagg aauauuacaa     2220

ucaauaaaaa uugucgauga acucguaaaa gugaugggag gccgaaaacc agaaucaaua     2280

guaguagaaa uggcacgcga aaaccaauau acaaaccaag gcaaaagcaa uucacaacaa     2340

agacuaaaaa gacucgaaaa aucacugaaa gaacuaggaa gcaaaauucu aaaagaaaac     2400

auaccagcaa aacuuucaaa aauagacaac aaugcccuac aaaacgauag acuauaucua     2460

uacuaucuac aaaaugguaa agacauguau acuggagacg accuagacau ugacagauua     2520

ucaaacuacg acauagacca uaucauccca caagcauuuc uaaaagauaa uucaauagau     2580

aauaaagucc ucguaucuuc agccucaaac cgcggcaaau cagaugacuu uccaucucua     2640

gagguuguua agaaacguaa aacuuuuugg uaucaacugu uaaaaagcaa acugauuuca     2700

caacgcaaau ucgacaacuu aacaaaagcu gaacgaggcg gacuacuccc agaagacaaa     2760

gcggguuuca uacagagaca gcuggucgaa acacgacaaa uuacuaaaca cguagcacga     2820

uuacuagacg aaaaguucaa uaacaagaaa gacgaaaaca acagggccgu acgcacagua     2880

aaaauaauua cauuaaaauc cacacuugug ucucaauuca gaaaagacuu ugagcucuac     2940

aaagugagag aaauaaauga uuuucaucau gcgcaugaug cuuauuuaaa cgcagucaua     3000

gcuagcgcac ugcucaagaa auauccaaaa uuagaaccag aauuuguuua cggagacuac     3060

ccaaaauaua acuccuuccg ugaacgaaag agugccacug aaaaagucua cuuuuauuca     3120

aacauaauga auauuuucaa gaaauccaua agccuagcgg auggacgcgu aauugaaaga     3180

cccuuaauag aagucaacga agaaacugga gaaagcguau ggaauaaaga aagcgacuua     3240

gcuacaguac gaagaguccu cuccuacccc caaguaaaug uagucaagaa aguagaagaa     3300

caaaaccacg gacuagacag aggcaagcca aaaggacuuu ucaaugcaaa ucucucaagu     3360

aagcccaaac caaacagcaa cgaaaauuua guuggagcaa aagaauaccu ugaucccaag     3420

aaauacggcg gauacgccgg aauaaguaac ucauucgcag ucuuaguaaa aggaacaaua     3480

gaaaaaggag cgaaaaagaa aauaacaaau guccuagaau uucaagguau aagcaucuua     3540

gaucgaauaa acuacaggaa agacaaacug aauuuccuuc uagaaaaagg uuacaaggac     3600

auagaauuaa uaauagaacu uccaaaauac ucucuauucg agcuaagcga cggaucacgu     3660

aggaugcuag cguccauauu aucaacaaau aauaagcgcg gugagauaca uaagggaaau     3720

caaauauucc ugucacaaaa auucgucaaa uuacuauacc acgcuaaacg aauaucaaac     3780

acaaucaaug agaaccacag aaaauaugua gaaaaccaca agaaagaguu cgaagaacug     3840

uucuacuaca uauuagaauu uaacgaaaac uauguaggag cuaagaaaaa cggcaaauua     3900

cucaacucag cuuuucaauc auggcaaaac cauucaauag augaauugug uucaucauuc     3960

auagguccaa ccgggucaga acgcaaagga cucuuugaac uaacauccag aggauccgca     4020

gccgacuuug aauuuuuagg uguaaaaauc cagagauacc gagacuacac accaagcuca     4080

uuacuaaaag augcuacuuu gauacaucaa ucaguaacag gccuguauga aacaagaauc     4140

gaccuagcaa aacuaggaga aggauga                                         4167


<210>  5553
<211>  4167
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5553
augaaaagaa uuuuaggauu agaucuuggc acaaauucca ucggaugggc acucguuaau       60

gaagcugaaa acgaaaaaga aauuucaaaa aucauuaaac uaggaguacg agucaacccc      120

uuaacgauug acgagaaaac caauuucgaa aaagggcguc cacuauccac caacgcggac      180

cgaacccuaa aaagaggugc aagaagaaau cuucaaagau auaaauuaag aagagaaaau      240

uuaauagaaa uacuaauaga caaucauauc auaacaaaag aaaccccuuu gacagaaauc      300

ggcaagggua caacauauca gacguuauca uuaagagcaa aagcaguaag agaacgcaua      360

gacuuagaag acuuugcaaa aguucuacuu gcaauaaaca aaaagagagg auauaaaagu      420

ucacgaaagg cuucgaauga cgaagaaggg caagcaaucg acgguauggc aauugccaaa      480

gaacucuaug aaagaggaau aacaccugga caauacguuc ucgaauuauu agaaaacgau      540

agaaaauaca uaccagauuu uuauagaagc gaucucaaac aagaauuuaa caaaaucugg      600

aauaaccaga aaaccuucua uccggaaaua cuaacagacg aauuauucaa agaacucgac      660

gguaaaaaca aaagucaaac uugggcuauc ugccaaaaac cuuuuaacau aaaaggaaua      720

aaacguauaa caaaaggacg agaaaagaca auagaaaacu aucauuggcg gguuaaaggc      780

uuaucaggaa aacugaacuu agaaaaauua gccauaaucc uacaagagau uaauaaugau      840

aucaaucaga gcucaggcua ucuaggaggu auauccgaca aaaguaagaa auuguaucua      900

aauaaagaaa cuguggguga auuucuuuau aagcaaauag ccaaaaaccc acauacaagu      960

uuaaagaacc aaguguuuua ccgacacgau uaucuagaug aguucgaaca aauuuggaaa     1020

acacaaucca auuucaacgc aaauuuaacu gacgaauuaa aacaagaaau cagagauguu     1080

aucauauuuu accaaagaaa aaucaaaucc cagaaaagcc uuauauccuu cugucaauuc     1140

gaaaguuggg aacaagaaua uuucgacaaa gaaacuggua aaaccaaaac acggacaguc     1200

ggaagaaggg uaaucccaaa auccucccca cuauuccaag aguucaaaau auggcaaaac     1260

cuaaaccacc ucgucuucac aaacagugaa acagacgaga aaauagaaau caaagaagua     1320

gaacaagaaa uaagaaaaga guuguuugaa gaacugaacc ucagaggcga ccuaaaucca     1380

aaugauuuac uaaaaauacu cagaucacaa uuuaaaauag guaaauuauc aaauuggaaa     1440

ugcaacuuug aaaagauaga gggaaaccgu acaaacgccg cacuuuuuaa caucuaccag     1500

aaaaucucag acuucgaagg auacgguauu gauuggaaca aguccaacgc uaaaaagaua     1560

agaaaagagc uaaaagcaac auuuccagaa auaggaauag acgcaagcau auuagaauuu     1620

gacgcauaua agaaacaacu cacaaaccaa aguagcuaug aacuauggca ccuucuuuac     1680

ucagcggaag aagacuucaa aauaucggaa caagauaaac uaauauacgg aaauucaucc     1740

gucucauuga aaaagaaauu acacuuaaaa uucggauuua acccugaaua ugcaaaaaug     1800

uuagcaaacc ucucacugau gagugauuac gguaaccucu cagcgaaagc uauaaagaaa     1860

auacuaccuu accuugaaga uggccaucug uauucagagg cuugcagacu agcagguuau     1920

aaccauucua aauccuuaac agcggaagaa cuugagaaau uugaacccaa ggcaaaacuc     1980

gacuuacuaa agaaaaacag ucuaagaaac cccgucgucg agaaaauuuu aaaccaaaug     2040

guaaacguug ucaaucaagu aaucgacaca uacggaaaac cagacggagu cagaauugaa     2100

cucgcaagag agcuaaagaa aagcgcaaaa gaaagagcug aaaugacaaa agguaucgcc     2160

gauggcacaa gacgcaacga agaaauaaag aaaaucauca cccaaaaauu ugguaucccc     2220

aacccaacaa aaagugaucu aguaagguac cgcuuauggc aagaacuaga aucacgagga     2280

uacaaagaca uauucaccga caaauauauc agcccugaua aaauauuuuc aagcgaaauu     2340

gacauagaac acauaauacc uaaagcccuu cuauucgacg auuccuuuag uaauaaaacc     2400

cucucguuua gaaacguaaa uuuaaagaaa gcaaaucgaa cagcccuuga cuucauaaca     2460

caagauuacc agagcaacgu agaagcauac aaagaacggg uugaaacaug gaacaaagac     2520

aaucacauuu caaaagguaa aagaaacaaa uuacuaaugu cacuaaaaga uauaccagau     2580

ggguuuauag aaagggaucu uagaaacucc caauacauag cacggaaagc uaaaagaaug     2640

cuuuacgaag uauucccaaa ugaaguucua gccacuucgg gaucaauaac agacaaauua     2700

agauccgauu gggauuuaau aaacguaaug aaagaacuua aucuaccaaa auacaaagcc     2760

uuagggcuaa cagaauacga agaacgacua gauaaaggca cagaagaguu auuuaaacac     2820

gaagucauca aagacuggac uaaaagaaau gaccacagac accaugcaau ggaugcacuc     2880

gcuguagcau ucacaaccca caaccauaua caauacauaa acaaucugaa ugcaagaaaa     2940

gacauuaccc aagauaauca uccaauaauu aaaaacaucg aaaaccuaau uacagaaguu     3000

aauuccaaag guaaacgagu auuuaaagcu cccaugucgu cauuuagaga auccgcaaaa     3060

gaacaaauag aagcaauauu aguaucauuu aaaaacaaaa auaaaguggu uacuaaaaau     3120

auaaaccaaa cuaagaaaga aggaggauuc aauuaccaaa augcuaaaac accuagagga     3180

caguuacaua aagaaacuau auacgggaaa aguaaacaac cgcuugaaaa accaguuaaa     3240

aucaacaauc gauucuguaa gaaacaaguc gggcuaauaa uuaauuuuaa agaacaagaa     3300

gcaguauuac aucaccugaa gaaauaugac aauaacgaga aaaucgcauu uucuaauaaa     3360

acccuuaaaa gugacccgau acaaguuaac gguucacccc uucgagaagu uaaaauauuc     3420

gaagaaauuu uuacaauaag aaaagacauc aauccagaca acuucaaaac ccaaaaacac     3480

uuagacaaaa uaaucgauaa aggaauaaaa gaaguaauga agaaaagauu agaagaauac     3540

aacggcgacu ucaaaacagc cuucuccgac uuagacaaaa aucccaucug gcuaaaucaa     3600

ucuaaaggaa uuacaauaaa aagagcaacu auaagcggag ugacuaaugc agaaccauua     3660

caccaagcua aagaccaccu aggaaauaua auuuuagacg auaacgggaa agaaauacca     3720

auagauuaug uaucgaccgg caacaaccac cacguagcaa ucuaucaaga cgaaagugga     3780

aaccuacaag aagaaguagu cucuuucuac gaagcaguaa uccguaaaaa ccaagaccaa     3840

cccauaauca agaaacgaca cccaaaagga uggaaauuuu uauucacuau gaaacaaaau     3900

gaaauguucg ucuucagcuc agaaaauuuu aauccaucag aaauagaccu acuggacgag     3960

aaaaacgcag caauuauaag uccaaaccug uuuagagugc agaaaauagc cacgaaaaac     4020

uauauguuuc gccaccaucu ggaaacagaa gucacaaaua auuuagacuu cacauaucga     4080

cauuuccaag caaccgaaug guugcgaaau cuaugcaaag uacggaucga ccauauuggu     4140

agaauagucc aaauagguga auacuga                                         4167


<210>  5554
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5554
augauaauaa gaacguauga acuuaaaaca auaacuacaa uugcaauacu aaaaauguuu       60

caacuacuca ccggagguuu aaguaugaca aaauuaggua aaccauacgg aaucggugua      120

gacauaggcu caaauagcau agguuucgcu gcugucgaug aaaacaguca ucucauaaga      180

cucaaaggca aaacaguaau aggagcccga uuauuugagg aggggaaagc agcagcagau      240

aggagggccu cgcgaacgac uagaagaaga cuaagucgaa acagauggag auuauccuuu      300

uuaagagacu uuuucgagag ucauaucaca ccaacagguc caaauuucuu uaugcgucaa      360

aaauacagcg agauaagccc gaaagauaaa gcuagguaca aauaugagaa aagacuauuu      420

aaugacagaa cugacgcuga auuuuaccaa caauauccca cuauguacca ucuaaggaau      480

agauuauuaa cggacccuuc aaaagccgau guaagagaga uuuauuuugc cauacaucau      540

auacuaaaau cuagaggcca cuuuuugaca ucggguuaug ccaaagacuu uaauacuaac      600

aaaguagcuc uuaacgaaau auuucccgca cuacaagacg cauaugcaca aguauaucca      660

gaccuagaca uaaccuuuga ugaaaacaaa guuaaugaau uuaaaacggu ucuuuuaaau      720

gaaaaagcca cuccaaguga cacacaaaga gcucuaguca aucuuuuacu ugcagaagac      780

ggagacaaag auauucugaa acaacaaaaa caaguacuaa cagaguucgc aaaagccguc      840

gucggacuca aaacaaaauu gaaugucgca cuagggaccg aaguggauag uucggaagcu      900

accgucugga auuucagucu aggacaacuu gaugauaaau gggcaggcau agaaucagcu      960

augacugaug aaggaacaga aauccuagac caaauaagag aucuauauag agcaagauua     1020

cuaaauggaa uagugcccgc cgguaaaaca uuaagccaag ccaaaguaga ugacuaugcc     1080

cagcaccacg aagaccuaga auuauucaaa gacuacuuaa aacaacuaga ggaugaugga     1140

acagcuaaag caauacgaca auuauacgac cguuauauug auggugacga cgcgaaacca     1200

uuuguucgag aagauuuugu caaagcacuc acaaaagaag uaacagcaca cccuaaugcg     1260

aaaaguccag aacuauuaga aagauuagcg caaccggacu uuauguugaa acaaaggaac     1320

aaagcaaaug gagcaauucc gguucaaaug caacaacgag aacuagacca aauaauuaag     1380

aaucagucag uguauuauga cuggcuggca gcaccgaacc caguagaaaa acaccgcaaa     1440

aguaugccau accaacuaga ugaacuacuc aauuucagga uacccuacua cguaggccca     1500

uuaguuacag caaaagaaca aaaagcggca caaggaggug uauuugcaug gaugguuaga     1560

aaagauccug acgguaauau uacaccuuau aauuucgaug aaaaaguaga uagagaagca     1620

agugcuaaua cauucauaca gagaaugaaa acaacagaca cuuauuuaau aggcgaagau     1680

guucugccca aacaaucacu acuauaucaa agauacgaag uacuaaacga gcuaaacaau     1740

guaaggguaa auaaugaaaa auuaagcaua gagcagaaac aacaaguaau acgagagcua     1800

uuugaaagac acaauuccgu cacaauaaag caauuugcug aaaaucuacg ugcacaugga     1860

gauuaugcac acauaccuga aauaagagga cuugcugacg agaaaagauu cuuaucauca     1920

cucucuacuu aucgacaauu aaaaucacua cuaccagaug cgauagauga cccagcaaag     1980

caagcagaca uagaaaacau aauagcaugg uccacuguau uugaagaugc ggcaaucuuc     2040

aaaaccaaac uaaaggaaau aaauugguua gauucgcaaa ccauuacaaa auuaucaaau     2100

auaagauaca gaggaugggg ccaauuuucc cauaaauucc uaaacggacu aacauuagga     2160

aauggacaua cuaucauaca agaauuauuu cuuucgaaca acaaucuuau gcaaauacug     2220

acagaugaaa cacuacaaaa gaaaaugaca gaauuaaaug cagauaaacu aaaagcggcu     2280

aacauuaacg augcaauuga cgaugcauac acaucaccuu ccaauaaaaa ggcuuuacgc     2340

caaguacuua ggguuauaga agacauaaaa cgugcggccg auggacagga cccgaguugg     2400

cucuaugucg aaacagcaga cgguggcggc acaccuggca aaagaacaag agcaagacaa     2460

caucaacugc aagaaauuua cgcaaacgcu gcacaugaac ugauagauuc cgcagucaga     2520

ggagaguuag aagauaaaau ugcagacaaa gccgauuuca augauaggcu agugcuauac     2580

uuuaugcaag ggggcagaga uauauauaca ggcacgccac uaaacauaga ccaauuaucu     2640

ucauaugaca ucgaccacau ucuaccccaa uccuugauaa aagauaacuc gcuugauaau     2700

agaguccuag uacacgcaau uauaaauaga gagaaaaacg ccaccuucgc auccacaauu     2760

uaugcacaga aaaugaacgc caccuggagg caauggcaug aagcuggcuu aauuuccgga     2820

agaaaauuaa gaaaucuaca gaugagaccc gaucaaauag auaaauacgc uucaggauuc     2880

gucgccagac aauuaguaga aacucgccaa aucaucaaac uaacagaaca aauaguugca     2940

gcucaauacc cagaaacaaa aauuauugca guuaaagcag gcuuauccca ucaacuccga     3000

gaagaauuag aguuuccuaa aaaccgcgac guaaaucacu aucaccaugc auuugaugcu     3060

uuccuggcgg cuagaauagg caccuauuua uuaaagcguu uucccaaccu acaagccuuc     3120

uuuacuuaug ggaaauuuaa gaaagcugac guuaagaaau uacgaggcuu caauuucauu     3180

agagauauca cacaugccga ggauaaaaua guaguaaaag auacgggaga aguaauaugg     3240

gauaaaaaga gagaugucga ugaauuagau agaauauaca auuuuaaaag aauguuaauu     3300

acacaugaag uacauuucga aaaggcggac uuauuuaaac agacaguuua cggcgcgaaa     3360

gacagcaaag aagcgggagg caagaaacaa cucauuucua aaaagaaagg auauccgguu     3420

gauauauacg gaggcuacac gcaggaaaca ggaucuuacu uaucaguagu gagauuaaca     3480

aagaaagcua uguacgcagu gguaaaagua aguacacgag acgcagcuaa auuagccgua     3540

gcaaaaucua uaagugaaca aaaagaaaau gagacacuaa acgauaucau agaugaaaaa     3600

uuaucgaaaa uaaguaaaaa cggaaaaauu acacacgaau uauucgaagu agucuuaccc     3660

cguguggguc agaaaacauu auuuaaaaac aguaaauaca accuauucuu ggugaacucu     3720

gauacauaua ugcauaauua ccaagaacuc uggaugccaa gagaauacca gaaaaugugg     3780

aaagauaucu ccuuaucaaa auauggugac gcgcaaacug aagaccaacu agaccaaauu     3840

uuuggauuca uaguaucuca aguuaacucc uauuucaauu uauaugauau aaaucaauuc     3900

cgaaagaaau uaaacgacgc ugcggauaaa uuugcaacac ucccuaugag agacacugau     3960

gauaugcaag gcaaaauagc aacaauugga caauuacuaa uuggccuaca agcaaaugcc     4020

gcaagaagcg auuuaagaaa uuuagacaua aaaaccccuu uaggccuccu acaaauuccu     4080

aacgggauau cgcucgacuc agacacauca guugucuauc aaaguccaac ugguuuauua     4140

gaaagagaag uacauuuauc agaccuauga                                      4170


<210>  5555
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5555
auggcaagag acuauucagu cgguuuagac auuggaacaa gcucugucgg uugggcagca       60

auagacaaca aauaccauuu aauucgagca aaaagcaaaa aucuaauagg uguacgccuu      120

uuugacucag cugucaccgc ugaaaaacgc cgaggauaua gaacuacaag aagacgacug      180

ucaagacggc acuggcgccu aagacucuug aacgacauuu uugccggucc auuaaccgac      240

uucggcgacg aaaauuuucu ugccagacua aaguauagcu gggugcaucc ucaagaccaa      300

ucaaaucaag cucacuuugc agcuggccua cucuuugaca guaaggaaca agauaaagau      360

uucuaucgca aauacccaac uauuuaccau cuacgauuag cgcuaaugaa cgacgaccaa      420

aaacacgauu uaagagaagu uuaucuugca auacaucacu uaguaaagua uagaggacau      480

uuccuaauag agggagaugu aaaagcagau ucagccuucg acguccauac uuuugcggac      540

gccauucaaa gauacgcuga auccaauaac ucugaugaaa aucuuuuggg aaaaauagac      600

gaaaagaaac ugucggcagc ccuaaccgac aaacacggau caaaaucaca gcgugcagaa      660

accgcugaaa cagcguucga uauucuugac uuacaaucua aaaagcaaau acaagccauu      720

cuaaaaucgg uuguugguaa ccaagccaau cucauggcua uauuuggauu agauucuucu      780

gcgaucagua aagacgaaca gaaaaauuau aaauuuuccu uugaugacgc agauaucgac      840

gagaaaauag cggacucaga agcauuacua ucagauacgg aguucgaguu ccuaugcgau      900

cuaaaagcag cguuugaugg uuugacauua aaaauguuac uuggagacga uaaaacagua      960

ucggccgcaa ugguucgucg auucaaugaa caccaaaaag auugggaaua cauaaaauca     1020

cacaucagaa acgcuaaaaa ugcaggaaau ggccuuuacg aaaagagcaa gaaauuugau     1080

ggaauaaaug cagcauacuu agcacuacaa ucugauaacg aagaugaucg aaagaaagca     1140

aagaaaaucu uucaagauga aauaucaucc gcagacauac cggaugaugu aaaagcagac     1200

uuccuaaaga aaauagauga cgaucaauuu uugccaauac aaagaaccaa aaauaacggc     1260

acaauacccc aucaacuaca cagaaacgaa uuagaacaaa uuauagaaaa acaagggauu     1320

uauuacccau uucuaaaaga caccuaccaa gaaaacucac acgaauuaaa caaaaucacu     1380

gcauuaauaa auuuccgcgu uccauacuac gugggaccuc uaguagaaga agaacagaaa     1440

auugcagacg auggcaaaaa uauaccagau ccaacaaauc acuggauggu acgaaaaucu     1500

aacgauacca uaacaccgug gaauuugagu caaguaguag aucuugauaa aucuggaaga     1560

agguucaucg aaagauuaac aggaacagau acauacuuaa uaggagaacc aacucuuccc     1620

aaaaauaguu uacuauacca aaaauuugau guucuacaag aacuaaacaa cauacgaguc     1680

uccggaagaa gacuagacau aagagcaaaa caagaugcuu uugaacacuu guuuaaagua     1740

cagaaaaccg uaucagcaac uaaccucaaa gauuuccugg ugcaagcagg auacaucagu     1800

gaagauacac aaauagaagg acuagccgac gucaauggca aaaacuucaa uaaugcauug     1860

acuacauaua auuacuuagu aucuguauua gguagggagu ucguugaaaa cccaaguaac     1920

gaagaacuac uagaagaaau aacugaacuu caaacugucu uugaagacaa gaaaguuuua     1980

cgucgacaac uugaccaacu agauggucua agugaucaua auagagaaaa auugaguaga     2040

aaacacuaca cagguugggg gagaauuagc aagaaacuau uaacgacaaa aauuguacaa     2100

aaugcagaca aaauugauaa ccaaacuuuc gauguaccac gaaugaauca aucaaucaua     2160

gauacacuau auaauaccaa aaugaauuua auggaaauca uaaacaacgc agaagacgau     2220

uuuggaguca gagccuggau agacaaacaa aauacgacag acggagacga acaagacguu     2280

uacagucuua uagaugaacu agcagguccu aaagaaauua aaagagggau aguacaaucg     2340

uuuagaauau uagaugauau aaccaaagcu guaggguacg caccuaaacg cgucuacuua     2400

gaguucgcua gaaaaacaca agaaucccau uuaaccaacu ccaggaaaaa ucaacucucc     2460

acuuuacuca aaaaugcagg ccucucagaa uugguuacac aagucagcca auaugaugca     2520

gccgcacuuc aaaaugacag auuauaucua uacuuucuac aacaagggaa agauauguac     2580

aguggggaaa aauuaaauuu agacaacuua ucuaacuacg auauagauca cauaauacca     2640

caagccuaua caaaggacaa cucacuugau aauagaguuc uaguaagcaa uauaaccaau     2700

agaagaaaau ccgauuccuc caauuacuua ccagcucuua uagacaaaau gagaccauuc     2760

uggucuguac uaucuaagca aggucuucua uccaaacaua aauuugcaaa uuuaacccga     2820

acaagagacu ucgacgacau ggaaaaagaa agguuuauag cuagaucauu agucgaaacu     2880

cggcaaauca uuaaaaacgu cgcuuccuua auagacucac acuucggugg agaaacuaaa     2940

gcaguugcaa uucggagcuc auuaacagcc gacaugagaa gauauguaga uauaccuaaa     3000

aaucgugaca uaaaugacua ucaucacgca uucgacgcuu uauuauucag cacuguaggc     3060

caauacaccg aaaauuccgg ucuaaugaag aaaggacaac uaucagacuc agcugggaac     3120

caauauaacc guuauaucaa agaauggauu caugcagcuc gccuuaaugc acaaucacag     3180

agaguaaauc ccuucggcuu cguagucggc aguaugagaa augcugcacc aggaaaauua     3240

aaucccgaaa cuggcgaaau uacgccagaa gaaaaugcug auuggucaau agcugaucua     3300

gauuaccuuc acaaaguuau gaauuucagg aaaaucacag uuaccagacg cuugaaagau     3360

caaaaaggac aacuauacga ugaaucccga uacccaucag ucuuacacga cgcaaaaagc     3420

aaagccucca uuaacuuuga uaaacauaaa ccaguagacc uauacggcgg uuuuucaucu     3480

gcaaaaccug cauaugcugc ucuaaucaaa uuuaaaaaca aguucagacu uguaaaugua     3540

uuacgacaau ggacauacuc cgauaaaaau uccgaagacu auauauuaga acaaauaagg     3600

gguaaauacc ccaaagcuga aaugguauug agccacaucc cauacggaca auuaguaaag     3660

aaagauggag cucuagucac uauaucauca gcaaccgaac uccacaacuu ugaacaguua     3720

uggcucccuu uagcagauua caaauuaaua aauacccuac ugaaaacuaa agaagauaac     3780

uuaguagaua uucugcacaa cagacucgac cuucccgaaa ugaccauaga aucagcauuu     3840

uauaaagccu uugacucaau cuuauccuuc gcauuuaaca gguacgcuuu acaccaaaac     3900

gcccucguga aacuacaagc ccauagagau gacuucaacg cacuuaacua cgaagacaaa     3960

caacaaacau uagaaagaau acuagacgca uuacaugcca gcccagcuag cucagauuug     4020

aagaaaauaa aucuaucauc aggauuugga cguuuauucu caccuucaca uuucaccuua     4080

gcagacaccg acgaauuuau auuucaaucg guuacaggau uauucuccac acagaaaaca     4140

guagcccaac uauaccaaga aacaaaauga                                      4170


<210>  5556
<211>  4170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5556
augaaaaacu auaacauauc ucuagucccg ucccccagug gugucggaua cgcuguaaca       60

gacgacaacc uaaacauaau aaaaccaguu caccauguau cgggaaucgg aagucguuua      120

uucaaugaag gaucaaguaa acaagauaga cgcaauucca gaucugccag aagauccaga      180

cauagacaac aaggacgaau aaaagcucug aacaguauaa ugaaagacga aaucauaaaa      240

guagacccca cuuucuuuga caggcuuuac caaagcgaac ucagcccacu ugacaaaaau      300

aagaaauauc gaaauauaau auucgauaaa ccuucagucg aaagcuauua uuacaagaaa      360

uucccaacaa ucuaccaucu ugaaaaauac cucauaaaca cagacgggaa agcagauaua      420

aggcuaauau acugggcacu ucacucucua cucacucaca gaggacauuu uuauaacacg      480

acaccagucu cgcaauuuaa accuggagca uuaaacauua aagacaaauu cauugaacua      540

aaucaacuua accaaguaaa aacauucgag uucucagucg caaacacacc cgaaaucgaa      600

cacauacuca aagacaggua uaucucaaaa caaaacaaaa uaaaccaacu cgcggaacuu      660

cuauuugaac aagaacccga aaaagacauc caaaaagcca auacuaaagu cgcaaaaacc      720

auagccaaag cuauacuugg aaacaaauuu caauuagaug aaauuauugg acucgacgua      780

aauaaauccg agaagaaaga auggagccug caacuaucag aucuuaaccu agacucaaaa      840

auagaugaac uuuccgauag ucuaacagau caacaauuaa gaauacucga aauacuacaa      900

gaaauguacg ccgccacaac auugcuagac auucuacaag gaucagauag ucuacuggau      960

gcaaaaauca aaagcuacaa ugaauacaaa caaaauagau uauuauucuu ugaucuucua     1020

aaaacccuuc cugauaagaa agcacaccuc uuaagacaag gcuacacccu auaccuuaac     1080

aaucaccauc gcgacuuagc uucugcucgc aaagacuuag ggauaguagc agcaacaaac     1140

uucuccaaaa cagacuuaua ugcacuuauc aagaaaacau ugaaagacga accagaaacu     1200

ccagaaagca agaaagucuu agggcuaauc gauaaaggcg aauuucugaa caaacaacga     1260

acagacuuaa acacuuacau uccuuaccaa cuaaacgcau uaauacuaaa uaaaauacug     1320

aaaaaccaag gcaaacacua uccauucuua auaaaagaaa auccagcaga accagauaga     1380

aaagaagcac cauaugaaau aucacaauua augcaauuua ccauuccaua uuacugcggg     1440

ccucuagcaa caccagaaga acagaaaaac cucccugaau caagcagauu cggguggcua     1500

gucaggaaag aagaaggcag aauuacaccg uggaauuuuu acaauaaaau caacgucaua     1560

gaaacagcua augcauucau uaaacgcucc auaggaaaag acaccuacuu acuguccgaa     1620

ccaguauuac cagacaauuc auuacucuac caaaaauaca aaguacuaaa ugaacuaucu     1680

ucgauuucuu uaguugacgc uaauaccaag aaagagaaga aacucacugc aaaaauaaaa     1740

caacuccuuu auaaagaagg guuuaagaaa cacgugaccg uaucaaaaac aauggcacuu     1800

aaaauauuga acgacaacaa cgucccaaca ucagauauac ucggacuauc aacaggcaag     1860

aaauucgaca acucccuaag cacauacaac ucauggaaga aagcauuccc agagcaaauc     1920

gacaauccuc auuaccguca agaccuagaa uauaugauag aauggucaac cgucuuuaca     1980

gauagaaaaa uucuaguuga aaaauuaaaa gaaauagagu gguugcgucc agagcaaauu     2040

aaauucguuu uggaacauag auuaacggga uggggaaaac uaaguaaacg ccuauuagug     2100

gaacucaaag auaauaaagg uaaaucaaac auagacaauu uauucaacac caagaaaaau     2160

uucaugcaaa ucguuagcca acaaguauau aauacucaaa uagaagcaau cgcgcuaaaa     2220

accaccaaaa accaaucccu cgauaacauc uuagaagcug ccuauacaag ucccgccaau     2280

agaaaagcaa uccgucaaac aaugaaagua guaaacgaaa ucguuacucu ggcagguaaa     2340

ggaacucacc cugauaaaau cuuccuuaca uuccaacggu ccaaagagca agaagguaaa     2400

uugacuacag auagagccuc aucgcuacuu aaaauauacc gaggaaucaa agacccacuc     2460

uguacuaaag agcuagugga ugaacucucc aaaaauguaa gagcaaaucu aauaaaaacu     2520

aaagaguacu uauauuauca gcaauuagga cgcgacgcau uaacugggga aaucauagac     2580

aaaacagacc uuagaaauua ugcaguacuu cacauaauuc cccgaagcgu aguaguagac     2640

gauucgauaa auaaccucgu acuuacuaaa gucaaaaaug caaaagaauc aauagucgua     2700

caauacggua gcaaaaacau cuccgacauu aguaugucag ucaaagacuu uuggaacaga     2760

uuacagaaac uaggcuuauu uucaaaagga aaacuaagaa accuuacaac ugacuuaaca     2820

gaacuaaauc aauacgaaaa acaagguuuu auagcaagac aauugguaga aacuaaccaa     2880

auagugaaac uucuugcaac uauucuacaa ggucaauucc cacauaccaa aauaauugaa     2940

guccgucaag aacaaauagc aaacauaaga uaucaacucg acauauaucg acuaagagau     3000

cugaauguau auuauagggg aauggacgcu uaucuagcag ccguuguugg aacguaccug     3060

uacacuguau acccaaaagc cagacgauuc uuuguuuaug gaaaauaccu caaacaggau     3120

aaaaacaacc gagaagaagg acaauucaaa acuuccggac auucauuuaa uuuccugugg     3180

aaauuauuuu augguggaga agacgaaaua uacguacaag gcacuaauaa cauagcauuc     3240

aauaagaaag aucucuugca gaaaauaaaa gaaguuaaua auuacaaauu ucaaaaugua     3300

uccuuagcaa cagguacuag aagguccgau uuauuuaaac aaacgauaua cccgcgacaa     3360

gagcgcgacg uaaagaaauc aagaacauua auaaccaaga aaaauggacu auucacugac     3420

auuuauggug gauacacagg aaacgucaac ucauauuucg uucuuguaca aacaaaauau     3480

aagaaagacc cagacaaauu caaccuuuau ggcguuccaa auagguaugu cgcaaugcug     3540

gaacaaucca aaucaaaagg uaauuaugaa accaaauuaa aagaagucau ggaaccuaua     3600

auuaaaaauu cgaacaagcg aaugauugga uauaaaauuc uaaaggauag aauauuauac     3660

aaucaaauaa uugaagacaa ugguuacaaa uucuuuauaa ccuccucaca auacaggcau     3720

aacuucagac aaaugauacu cagcgaccaa ucuaagaaaa uucuaaugga uuaugucuuu     3780

gacccaaauu ucuauagaca uaagaaaacc gaggacguaa auaaugacaa agacgcuaaa     3840

cucauuaaag ucuacgauga aauauuauac caaauagauc auuaccuuca aauauucaca     3900

aucggacauc auauagauaa auuaaagaaa gcacgcccgu uguuugaaaa auguacacua     3960

gccgaaaaag cacaaguauu aaacgauaua uuaauaauug cuggcucuaa ccaaaccgaa     4020

uuagcacuaa aaccauuaaa aacaacuaaa cuaacauucu cgaaacaaaa cuguccauug     4080

acaaaagacg caaucuucua cuaccaaucu ccuaccggaa ugauaacaag aaaagaaagu     4140

aucaaagaua uacucaaaca aaacauuuga                                      4170


<210>  5557
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5557
augagucgca aaaacauagu ggaagacuac gcaauuucgc uugacauagg uaacgcaucu       60

gugggauggg uugcauuuac accaaacuau agacuuguaa gagcuaaagg ucaugaauua      120

auuggagcca gacuguucga gccagcguca acggcggagg aaagacgaau ggcucgaaca      180

accaggagac gauauucucg uagaagaugg agauuacgaa uguuagauag cauguuugau      240

caagcucuaa gugaaguaga uccaucguuu uugaugagaa gaaaauauag uuggguacau      300

ccagaagaua aaaauaacga agcccauugg uuuggcggag uauuauuuga uaguaaugaa      360

caagauaaaa gauuuuacga gaaauaucca acuauauacc aucuacgaaa aaguuuaaug      420

gaagaugacg aaagacauga uaucagggaa guuuauuuag caauucauca uaugaugaaa      480

uaucgaggaa auuuccuaac ggaaggugaa uuagcuccau caaguguguu cgauguugag      540

gauuuccuaa aauuauuaac agaaauacuc caauaugcag augaaggcga aacagaugaa      600

gauuuagaau uacaagcaga cgcuaaugga cuaacagaag cccuuuguac cacaaaaggu      660

ucaagaagca uacgagccaa aaaugcaaua ucgauucuaa aagaaggggc agcauuaucg      720

aaaagacaag aagcaauaau uucagcagua uuugcaggac uagaagggaa caaauuggac      780

cuaacaaaag uauuuccaaa caaagaauau acacccgaag auaagaaaac ucucgcacua      840

uauuucaaca aagccgauua ugaugaaaaa cgaguagcaa uaggagauag uggauuauua      900

gaagauaaug aaugugacuu ucuugauugu aucagaagac aauacagugc aauagcacuu      960

aaacaacucc ugggagacuu cacuucaauc agcgaaucua ugugugccag auacgaacaa     1020

caccaacgaa auugggauuu aauaaaacga uacuguaggg aagaaaguaa ugcagaaaau     1080

aucaauaaaa acuauggcgc acuugucggu ugggaaaucg ucgguggaca aagaagauca     1140

auaagagggg cgaaagacua cgaaaacaag agaaaagcag caaacaaauu cuuugaagaa     1200

cuuauaaaag gaagcgcguu gccagaccaa gaaaaagaaa gauuauuaca cgacauagaa     1260

aacgacgaac uauucccagu acaacgagac uccggcaaug ggguaauucc auaccaacuc     1320

cacuugaaug aacugaagaa aauaauccaa aaacaaggcc guuauuaucc cuuccuacug     1380

gaaacauaca uaaaagacgg aaaagaagua aacaaauuag aaggacuauu ggcauucaga     1440

guaccuuauu acguuggacc auuaguauca agagaugaua ugcagucuag ugauaauaga     1500

gaaaaucauu ggaugaucag aagaaagaaa ggaguaauaa cacccuggaa uuuugaugaa     1560

augguugaua aagacaaauc aggacacgag uuuauaaaau cauuaguagg aaccgacagc     1620

uaccuuuuag gugaaacaac ccucccuaaa aacagucuac ucuaucaaga guucgaagua     1680

cugaacgaac ucaauaacgu uagacuaagc auucggacag gaaaccauug ggcagauaaa     1740

cgaaggguga gauuaggaca uaacgagaaa aaguuguuac ucgaagaauu auucgcuaaa     1800

caagcuacag ucacaaagaa agcugccgaa aaucuauuaa gaagaaccua ugguaacacg     1860

uaugagauaa guggcuuagc ggaugaaaag aaauuuguuu caucccugag uucauauggc     1920

aaaauguguc gaacauuaaa agcaaguuau gucaacucac auaaagacgu uguagagaaa     1980

auuauagaac uacaaacagu auuugaagac aagaaaacac ucaaacauca gcuacaacua     2040

uuaggagaac uagcugaaac caacugcgaa cuacuaucaa acacacacua uacagguugg     2100

ggaaaacuca gcagaaaacu acuaaccuca aaaguaguug ucugcaaaau aggaaaugac     2160

uucgccccag caaaacacuc cauaauagaa auaaugagag aaacagaccg aaacuucaug     2220

gaaauaauaa cggacaaaga ccugggagua ggugaaugga uaaaccaaca aaacguagga     2280

gccgaagaug gcaauaguua ccaagacaua auagacgauc uuagagucag uccaaaaguc     2340

aaaagaggua ucaaucaagc aauaagagug auugacgaua uaaguaaagc aguagguaag     2400

gaaccauccc gcauauuccu ggaacuagcu ggugacgugc aacaaagcgc aagaacugua     2460

ucuaggaaaa gucgacuaca agguuuguau aaaucugcag gauuacaaaa agaguucaga     2520

gauuuagcug aaagucuaaa ugaguguuca gauaacgauc uacgcgauga uagacuauuu     2580

cuuuauuaua cacaguuagg aaaggauaug uacacugggg aaccacuaaa ccuagauaga     2640

cugaguagcg acuaugauau agaucauaua auaccacagg cugucacuca aaaugacuca     2700

uuagacaaca gaguuuuggu agcucgggua gcaaaugcaa ggaaaacuga uucguuucaa     2760

uaucucccug agaucgccaa uagaaugcgu aauuuuuggc aagaguuguu agaugcaggu     2820

cuuauaucac gaguaaaauu ugauagacuu aacagacaaa acgauuucuc aucacaugaa     2880

aaagaacggu ucguucaaag aucuuuagua gaaacacgac aaauuaugaa aaauguugca     2940

acaaucaugc gaaaaagaua ugguaauagc gcagcuguaa uagggcuuaa uagugaauua     3000

acuaaagaaa ugaggcaaua ccuaggauuu ucgcacaaaa auagagacau uaacgauuac     3060

caucacgcuc aggaugcacu uugccucgga auagcaggcc aauuugccgu caauagagga     3120

uucuuugcua auggaauggu uucagaugga gcgguuaauu cauauaaucu auaccuacag     3180

gauuaucuua ggggauacag ggagaaaaug agcgcaggag aacgaagaag aaacaaagca     3240

uuuggcuuca uaguuggauc caugaacuca caagauagug acaaacggau aaauccaaaa     3300

acaggagaaa uagcauggga cgacgaagau aaagucuacc uaaggaaagu uaugaacuac     3360

cgcaaaaugc ucauaacaca aaaaguagga gauaguuuug gagcacucua caaugaaacu     3420

agauacggaa caagcgcaaa agaaggagua aacggaauac cauuugauaa gcauaagcaa     3480

gauacagcuu uguauggagg auucagcuca gcaaaaacug uauauguagu gcuuauugaa     3540

gccaagaaaa agacuagguu agucaacaua accaugcaag aauacgcaga acucggugac     3600

cauccaagcg acgacgcacu ccgcgcugua cuugcgaaaa agaaacccga auauggaaag     3660

gcaagaauac uauuaagaca cauuccccca augcaacuaa uaagauacgg uggagcauua     3720

guaacaguua aaagcgcaac agagcuuaac aacgcgcaac aauuauggcu cccauacaaa     3780

gaauacuguc uguuugauga cgucauaucu ucagcuauag auguagauga agccagacua     3840

ugccaaaugu uagauguaau ucucgacgca auccaaaaau acuacccaaa acagcguuuc     3900

gaugagacug gauugucugc auuccacaau gcuuucaaug aaguaucagc agaagauaag     3960

aaaagaauug uaggagaaau ggucggaaug cuucacgcag augcuaaaaa cgcaaaucuu     4020

aaagcauuag gcaugagugg acgaugggga cgaaugucca aucagucagg cuacacuuuu     4080

gcagacucug augaguuuau cuuucaaagc ccuucaggau uauucgaaaa acguaucucu     4140

guaggugaau ugaagaaaag acgagcaaga uga                                  4173


<210>  5558
<211>  4173
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5558
augaaggaag uuaaggauua caucauagga uuagacauug gaacaaacuc uuguggauau       60

gugguaacgg acaaacaaaa uaauauacua aaacuaaaag ggaagacugc uauuggagca      120

cgacuuuuua aagaagguga agcagcagca gaucgacgug cauucaggac uacaagaaga      180

cgccucgcga ggagacgaug gaggcucgga cucuuagaag aaauauuuga cgaagaaaug      240

gcuaaaguag acuccucauu uuuuagaaga cugaaagaau cagauuacuc accaaaagau      300

ucccguaaac aauucaacuc cauaguuuuu gaaagcagug aagcagauaa agacuucuac      360

gauaaauauc caacuauaua ucacuugaga aaugcgcuaa ugcacgacga ccaaaaacau      420

gauuuaagag aaauuuuucu ugcaguucac cauauaguaa aauacagagg aaauuuccua      480

cgcgaagacu cuguaaaggc cuuuaaagcc gcuaaauuua cacuacaagg cgaagaugga      540

auaggaccag ucgacaaacu uaaugacuua cucaaagaaa ucuauaucga ggcagcucca      600

gaacuugcaa cggauaaccu uucaaagauu gaagaaauaa uaaaagauaa aaaacucuac      660

aagcaagaca aacuaaaaca aguugcaacu uuacuaacaa aagaagccga uacaaaagac      720

aaagcaaagc uuaacaaaga cguagcaaag caagucgcaa augcauucau gggauauaug      780

auaauauuug auacaauauu caaccuuucg gauguugacu caaaagauua uaaaguuaaa      840

uuuagcgaug caaauauuga cgagaaacuu gauacauuaa cuucucuacu uacagacaaa      900

caaacagaau uuguccuaga auuacaaaau auauauaaua cuauaguccu uaacgaaauu      960

gucccugacg gcaaaagcuu auccgaaagu augguuaaga aauaugacga ucacaaggag     1020

gaccuaaagu uauuuaaagc auaucuaaac ucccuuacag acguaaaaaa aguaaaagag     1080

cuaaaagcag cauauguauu guacguuaau aacagaaaag cgaaccuacu agccgcaaaa     1140

aaacuacuug gaaaaaaagc ugauaauaug aacaauuuug aaauuuucuc caaauuugua     1200

gcagauaauc uagaugacuc agaacuagcc aauaagauaa gagcacgucu ugacuuaggu     1260

gaauuucuac ccaaacaacg aacuaaccaa aaugggguaa ucccauacca auuacaccaa     1320

guugaacuaa cacaaauacu ugagaaacaa ggcaaauacu acccauuuuu aaucgcaccu     1380

aacccagucg aaucacauag aaacaaugca cccuacagaa uaagugaacu uguauccuuc     1440

cguguaccau acuaugucgg accauuaaua gacccuaaua augcagaauc agaacaagac     1500

aaagcaaacu cgagcuucgc cuggauggua cgccaaaaac aaggacaaau aaccccuugg     1560

aauuucgaag aaaugguaga cacaaccgaa ucugcgaaua aauuuaucaa aaggaugacu     1620

acaaaagaua cuuaccuccu cggcgaagac guacuucccg caucaagccu cauauaucaa     1680

aaauucguaa uacucaacga auuaaacaau auaaagguua auggaauaaa acucacuucu     1740

gaagaaaaac accagauauu ugaugaacuu uuuaaaguac aaaaauccgu aagucuaaaa     1800

aaccuuguaa acuuccuaac agcacacggc ugcguagcgc caaaugucac uggcuuaucc     1860

gacggaacaa aauuuaauuc uucuuauuca acguauauag auuaccggaa aauauuuggc     1920

gccgagauug acaacaaaga caaacaagcg gacuucgaaa aaaugauaga guggucaaca     1980

guauuugaag auagaaaaau uuucaaaaga aaacuugaag aaauuaccug gcucacacca     2040

gaucagauaa aacaagucuc aaguaaaaga uacagcgggu ggggaagacu aucuaaaaaa     2100

cuacucacgc aaauaacaga cgaaaauggu guaaauaucc uacaaagacu auggaacgaa     2160

agugagaacc uuaugcaaau acuaagcgac ccaguaauaa aagaaaaaau uguagcagcc     2220

aauagcucuu uuacaaaggc aacggacaug gaagauauau uagcugaagc uuacacaucu     2280

ccacaaaaua agaaggcaau acggcaagua augcgggucg uagacgacau aauagcagca     2340

gcccacggua aaaaacccag ccaaauagca auugaauuug cgcgugaaga ucaagacaac     2400

ccucaaauga ccaaaagucg aaaagcacaa uuagaccaaa uauaugcaaa aauuagcgac     2460

gaauucuuag acaacgcugu aaaaaacgaa cuaaaaaaua ugaaagauaa uaaaccuuua     2520

gcuaaagaua aaguuuuuuu auacuuuaaa caaaugggca gagacgcaua cacaggagag     2580

aaacuaagcc ucgauaaucu ucaagacuau gauauagauc acaucuaucc ccaauccuuc     2640

auaaaggacg auuccuucga uaauagagua uuaguacaga gagcaguaaa caacggaaaa     2700

agugauaaug uuccaguccu acuuuucgga aacaaacaag cagcggaucu agguaugacu     2760

auaagacaaa uguggaaaaa guggcuugaa uuagggcuaa uauccaagag aaaauucaau     2820

aaucuaauca cagacccuga aaaaauaagu uccuacaugg cuagcggauu cgugaaccgc     2880

caauuaguag aaacaucgca agucauuaaa cuaguagcga acaucuuaca agcaaaacuc     2940

ccugacacag aaauaauaga aguaaaggcu ucuuauaaca gcauucuaag aaaagaauuc     3000

aauuuauaca aaucuagaga agugaacgau uaccaccaug caauugacgc guacuuaacc     3060

acuauaguag gcaacuaucu uuaccaagua uauccuaaau uacgacccua cuucguauau     3120

ggacaguuua aaaaauuugg aaaagcagaa uccaaagaag acgauccagu caagaaaauc     3180

aaacaauuca auuuuauuua ccaacuucua ugggguaaag augacgcgau auguauauca     3240

cauacugauc aacaaguuuu uagccguucu gauauaauca auaaauuaac cacagcauau     3300

aacuuuaaau acaugaaugu cucacaagaa guuuacacca auagcaauaa cguauucaaa     3360

cagacaguau ucccaauaau cgaacgugac acagcuaaau cgagaucucu aauaccaaaa     3420

aaacucgauc gaccuacaga cauauacgga ggauauucaa acaacaacga cgcguaccuc     3480

gcacuaguaa aaguagacaa aaaaaaggga uccgaauaca gagucgucgg aauaccuaug     3540

agagcugcag cgauguuaaa agccucaaaa aauuaugacg aggaucuuag aaaaguaauc     3600

gagcccaugg uaaugcuuga uaaaaauggc aaagcgaaac gaggcauaga aggauuuaga     3660

guucucguag gaaaaaugcc cuucaaacaa gccgucuuag auggagauaa gaaauuuaug     3720

uuaacaggua caaacgacau gccaaaucua aagcaacucg uucuaucgca gcaaaauaug     3780

aaauacauau uagacuacau agaugauaua gauugcagaa aacauaaauu uggaaacaaa     3840

acucaagacg gaaauaaaug cuuacuagaa guauuugaug aaauaauaca aauuguagac     3900

aaacauuuug aauuauucga caauagaucc uucagagaaa aacuccgcaa cucgagaguu     3960

aacuuuauaa accucgcuuu aucuaagaaa gcagaagccc uacuugccau ucuaaacgga     4020

cuacaugcua auaguaugag aacaaccauc aaagaacuga aaauauccga ccuuggacgg     4080

auccaaucau ccgugaucuc accagaagca caauuaauuu aucagucacc cacaggccuu     4140

uucgaaagga gaguaaaggu uaaagaccua uga                                  4173


<210>  5559
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5559
auggaaaaua aacaauacua cauuggacuc gacgugggaa caaauucugu uggaugggca       60

guuacagaca caaguuauaa ccuuuuaaga gcaaaaggaa aagauaugug gggagcaagg      120

uuauucgaaa aagccaauac ggcggcugaa cgccggacaa aaagaacaag ucgaagacga      180

ucagaaagag aaaaagcaag aaaagcaaug cuuaaagaau uauuugcaga cgaaauaaau      240

cguguagauc caaguuucuu cauacgacuc gaagaaagua aauucuuucu agaugaccga      300

ucagaaaaua acaggcaaag auauacauua uuuaacgaug caaccuucac cgacaaagac      360

uacuaugaaa aauacaaaac gaucuuccac cucagaucag cauuaaucaa uucagacgaa      420

aaauucgaug uaagauuggu auucuuagca auucuaaacc uauuuagcca caggggacac      480

uuucuaaacg caucucuaaa aggagacgga gacauacaag gcauggacgu auuuuauaau      540

gacuuaguag aaagcugcga auacuucgaa aucgaacuac caagaauaac aaauauagau      600

aacuucgaga aaauccuauc ccaaaaaggc aaaucacgaa caaaaauacu agaagaacua      660

agugaagaac uaaguauaag caagaaagau aaaucuaaau acaaccuaau aaaacuaaua      720

aguggacucg aagcauccgu uguagaacua uauaacauug aagacauaca agaugaaaau      780

aagaaaauca aaauaggauu ucgcgaauca gacuacgaag aaaguucgcu uaaaguaaaa      840

gaaauuaucg gggacgagua cuucgauuua gucgaaagag caaaaagugu acaugauaug      900

ggauugcugu caaacaucau aggaaauuca aaauaccuau gcgaagcaag agucgaggcu      960

uacgaaaauc aucacaaaga uuugcugaaa auuaaagaac uauuaaagaa auaugacaag     1020

aaagcauaca acgauauguu cagaaaaaug acagauaaaa acuacucggc auacgucggu     1080

ucaguaaauu caaauauagc aaaagaaagg agaagcguug auaaaagaaa aauagaagac     1140

cuauauaaau acaucgaaga cacagcacuc aaaaacauac cagacgacaa caaagacaaa     1200

auugaaauac uagagaaaau aaaacuagga gaauuucuaa agaaacaguu aacggcguca     1260

aacggaguaa ucccaaacca acuccagagu agagaacuaa gagcaauccu aaagaaagcu     1320

gaaaacuauc uaccauuucu gaaagaaaaa ggagagaaaa auuuaacugu uucugaaaug     1380

auaauccaac uauucgaauu ucaaauccca uauuauguug gaccccuaga caaaaauccg     1440

aagaaagaua acaaagcaaa uucaugggcc aaaauuaaac agggagguag aauacugcca     1500

uggaacuucg aagacaaagu cgauguuaaa ggcagucgua aagaguucau agagaaaaug     1560

gucagaaaau guacauauau aucagacgaa cacacucugc cgaaacaguc acucuuauau     1620

gaaaaauuca ugguauugaa cgaaauaaac aauauuaaaa ucgacggaga gaaaauauca     1680

guagaagcua aacagaaaau uuauaaugac cuauucguca aaggcaagaa agucucucaa     1740

aaagauauaa agaaagaauu aauaaguuua aacaucaugg acaaagacag uguacuauca     1800

ggaacagaca cagucugcaa ugcauaccua ucgucuauag ggaaauucac agguguauuc     1860

aaagaagaaa uaaacaaaca aagcauagug gacaugaucg aagauauaau auuccuaaaa     1920

accgucuacg gggacgaaaa acgauuugua aaagaagaaa uuguugaaaa auauggugau     1980

gaaauagaca aagauaaaau aaaacgaauc uuaggcuuua aauuuucaaa cuggggaaau     2040

cucucaaaau cauucuuaga auuagaagga gccgacgucg ggaccggaga aguuagaagc     2100

auaauccaaa guuuauggga aacaaacuuu aauuuaaugg aacuccuaag cucaagguuc     2160

accuauaugg acgaacucga aaaacgaguc aagaaacuag aaaaaccauu aucagaaugg     2220

acaauugaag accuugauga cauguacuua aguucaccag uaaaaagaau gauauggcag     2280

ucuaugaaaa ucguugauga aauacaaaca guaauaggcu acgccccaaa aaggauauuu     2340

guagaaauga cuagaucaga aggugaaaaa guaagaacaa aaucccggaa agacagacua     2400

aaagaauuau acaacggaau aaaagaagac ucaaagcaau ggguaaaaga acucgacuca     2460

aaggacgaaa gcuacuuuag aucgaaaaag auguauuugu auuaccucca aaaaggacga     2520

ugcauguaca gcggagaagu aauagaauug gauaaacuaa uggaugacaa ccucuacgac     2580

auagaccaua uauauccucg aaguuucgua aaagacgauu ccuuggacaa ucuaguucua     2640

guuaaaaagg aaauaaauaa caggaagcaa aaugauccaa uuacuccgca aauacaagca     2700

ucaugucaag gcuuuuggaa aauucuacau gaccaaggau ucaugucuaa cgaaaaauac     2760

aguagacuaa cccgaaaaac ccaagaguuc agcgaugaag aaaaauuauc auucaucaau     2820

cggcaaauag uagaaacagg acaagcuacu aaauguaugg cccaaauuuu gcaaaagagu     2880

augggagaag acguagaugu cguauucagu aaagcaaggc ucguaucaga guucagacau     2940

aaguucgagc uauuuaaaag cagguuaauc aacgauuucc aucacgcuaa cgacgccuau     3000

uuaaauauug uaguaggcaa caguuauuuu guaaaguuua caagaaaucc agcaaauuuc     3060

auaaaagaug caagaaagaa ccccgauaac ccuguauaua aauaucacau ggacagauuc     3120

uuugaaagag auguuaaauc caaaagcgaa guugcgugga uaggacaaag ugaaggaaac     3180

uccggcacga uaguaauugu caagaaaaca auggcuaaaa acucgccauu aauaacaaag     3240

aaaguagaag aaggacaugg aucaauaacu aaagaaacga uugugggagu caaggaaauu     3300

aaauuuggua gaaauaaggu ugagaaggca gacaaaacuc ccaagaaacc aaacuuacaa     3360

gcauacagac ccauaaaaac aagcgacgag agacucugua auauauugag auauggcgga     3420

cgaacaagca uaucaaucag cggcuacugu uuaguagaau auguuaagaa aaggaaaaca     3480

auacgauccc ucgaagcaau accaguuuac uuaggaagaa aagauagucu aucagaagaa     3540

aaacuacuaa acuacuucag auauaaucua aacgacggcg gaaaagauag uguaagugau     3600

auaagauuau gccuaccauu uauaaguaca aacucccugg ugaaaauaga uggauaucua     3660

uauuacuuag gagguaaaaa cgaugacaga auacaacuau acaacgcgua ucaauuaaaa     3720

augaagaaag aagaaguaga guauauccga aaaauagaaa aagcaguaag caugucaaaa     3780

uuugaugaaa ucgauagaga gaaaaaccca gugcucacag aagagaaaaa uauagaacua     3840

uauaauaaaa uacaagauaa auucgaaaau acuguauuua gcaaaaggau gagcuuagug     3900

aaauauaaua agaaagacuu aucauuugga gacuuucuca aaaacaagaa auccaaauuc     3960

gaagaaaucg acuuagaaaa acaauguaaa guacuauaua acauaaucuu uaaccuaucc     4020

aacuuaaaag aaguugacuu gagugauauc ggugguagca aaaguacugg aaaauguaga     4080

uguaagaaaa acauaacgaa cuauaaagag uucaaacuca uucaacaauc cauaacagga     4140

uuauauuccu gcgaaaaaga ccuaaugaca auauga                               4176


<210>  5560
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5560
augaaagaaa ucaaagacua cauuauuggu cuagauauag gaaccaacuc cugcggauau       60

guaguaacug acaaacagaa caacauacua aaauuaaaag guaaaacagc uaucggugca      120

cgacuauuua aagaaggaga agccgcggca gacagacgag cguuuagaac aacacgacga      180

agguuagcac gcagaaggug gcgauuauca cuccuagaag aaauauucga ugaagaaaug      240

aauaaaguug acccaucauu uuucaggcga uuaaaagaau ccgacuacuc cccgaaagac      300

cgaagaaaac aauuuaauuc aaucguauuu gaaucaucag aagcagauaa agcauuuuau      360

gaaaaauauc cuaccauaua ccaucugaga aaugcucuua ugcaugauaa ucagaaacau      420

gaccuuagag aaauauuccu ugcuguacac cacauaguca aguaucgagg uaacuuccuu      480

cgugaagauu caguaaaagc auucaaagca gcuaaauuca cucuacaagg ugaagaugga      540

auaggucccg uugauaaauu gaacgaccuc cuaaaagaaa ucuacaucga agcugcccca      600

gaacuagcca cagacaaucu aucuaaaaua gaagacauaa ucaaagacaa gaaacuuuau      660

aaacaagaca aacuaaaaca aauagcaaau cuucucccaa aagcuguaga uuccaaagac      720

aaagcaaaac uuaacaaaga uauagcaaaa caaguagcaa augcccuaau gggauauaug      780

uuuagguuag acacuuuauu uucauuuaca gacguagaug uuaaagauua caaauuaaaa      840

uucuccgacg caaacauaga cgaaagccua gacgcacuua caucucuauu aacagaugcc      900

caaauagagu ucguccucga acuacaaagu auauauaaua caauuguuuu aaacgaaaua      960

guccccgacg guaugucucu cucagaaucu auggucaaga aauaugauga ucauaagaaa     1020

gauuuaaaac uauauaaaga auacaucgac uccuuaucag aucagaagaa agcgaaacaa     1080

uuagaagcag cguaugcucu auacguaaau uaccguaaag cagaucuacu agcugcuaag     1140

aaacuauuug agaaaaagaa aauaggagac aagaaauucg uagacgugau auccaauuuu     1200

gaagucuucg ggaaauuugu aagcgacaau uuagaugacu cagaauuagc aaauaaaauc     1260

aaagcuagac uugaccucgg cgaauuucuc cccaaacaac gcacgaacca aaacggaguu     1320

auaccauauc aacuccauca aguagaacua acgcagaucc uagaaaaaca agggaaauac     1380

uauccauucu uaauaacacc caacccagua gaaucacauc guaauaacgc uccuuaugaa     1440

aucucugaau ugguauccuu cagaguacca uacuacguag gcccacuaau cgauaaucaa     1500

uccauaaaag auaaacagaa uaaaaacaaa uucgcaugga ugguacgcca aaaacaaggu     1560

caaauuacgc cauggaauuu cgaagaaaug guggacacga cugaaucugc aaaucaguuc     1620

aucaaacgua ugaccagaaa agacacguac cuucuugcag aagacguucu ccccaaauca     1680

ucccuaauau accaaaaauu caugaucuua gacgaacuca accgaauuaa aauagacggc     1740

aagaaauuaa ccuccaaacu aaaacacgau auuuucgaaa aauuguucaa gaaacagaaa     1800

aguauaaauc uagacaaucu caaaaauuac cuacuagcag aaggaaacau acccggccuc     1860

auagaaggac uuucugacgg uauaaacuuc aauaauucau uuuccacaua cauagacuac     1920

cgaaacauau uuggagauga aauugauaau ccaaacaaac aagcagacuu cgagaaaaug     1980

auagaaugga gcacagucuu cgaagaccga aaaauauuca aaagaaaacu gaaagaaaua     2040

acauggcuua ccccugaaca aauaaaacaa guuucaagua aaagguacuc cggauggggg     2100

cgacuaagca agaaauuacu uacacaaauc accgaugaaa acggaguaaa cauauuacaa     2160

cgacuaugga augaaccaga aacuuuaaca gaaguccucg cuaauccagu aauuaaacgc     2220

aaaauaagcg aagcaaauuc auuauucgua caaaucaaca aaguagaaaa cauauuagac     2280

gacgccuaua cuucuccaca aaacaagaaa gccauuagac aaguaauccg uguuguagau     2340

gauauuauag cagcggcaca uggcaagaaa ccaagucaaa uagcuauaga guucgccaga     2400

aguucacaaa acaaagcaaa agucccugac acaagaaaga aacaacuaga uaaaauauac     2460

aauaaaaucu cuucggaaau auuagacucu ucuaucaaaa augaacucaa aaauuuaaaa     2520

ucaaauaaau aucuaagcaa agauaaauua uuucuuuacu ucaagcaaau gggacgagau     2580

gcauacaccg gagauaaauu aucacuagac caauugcaaa auuacgacau cgaccauaua     2640

uuuccuagau cguucaucaa agacgacuca uuagacaaua ggguacuaac ucaaaaacca     2700

auaaacgcua aaaaguccga uuacggaaua ccagcacuag aauuuggcaa caaauauguu     2760

ccagaucuag gcaucaccgu caaagaaaug uggaaauuau ggcaggaaaa cggauuaaua     2820

ucuaaaagca aauuaaucaa ccuauguacc aaccccaaaa agauaggcuc caaaagagcc     2880

agcggauuca ucaacagaca gcuaguagaa acuucccaag ucauaaagcu aguugcgaua     2940

auacuccaag ccgaguuccc agacacagaa auaauugagg ugaaagcccu acagaauaca     3000

acauuacgag aaucauuuca ucuauacaag aacagaucug ucaacgacua ccaccaugca     3060

auugaugcau acuuaacaac uauuguuggu aacuaccuuu aucaaguaua cccaaaacua     3120

agaccuuacu ucguuuacgg ccaauucaag aaauuuaacc aagagaaaaa uauagauauc     3180

cugaaaagac ucaaaaacuu uaacuuccua cgacaauuaa ucuucaauac agaugauaau     3240

auauacaucu ccggaacgaa agaaauaguu uucaacaaga aagacaucau ccauaaauug     3300

gaaacagcuu auggcuacaa guacaugaau auuucaaggg aauguuauca agaaacuucg     3360

ucucuauuca aucagacacu auaugcacac aacucaagug uaaagaacuc ucuaauaccc     3420

aaaaagaaag gcuuacccac agaaauauac gguggcuauu cagggaacaa agauucauuc     3480

uuuguccuag uaaaaaucgu aaagaaacga accaacuuau auagaauagu aggcauccca     3540

acucgcgaau uggcaaaauu aaacagcucc aauaacuaca aucaagcacu aaauaaaauu     3600

guugaaucaa aacuaugucu aaaagaaacu gagagcuuua aaauccuaau aaaacgccuc     3660

cucuauggaa cacucauugu cgacaacgga cagaaauuuc gcauaggauc uuuuaaagaa     3720

aaacacaacg uacaacaacu uguauuacaa cucaaaucaa ugaaauacau caaauuuuau     3780

aucgacggcg gacaaaacua cuucacagau guagaaagaa agaaauuaga gaaacaagac     3840

agagacaagu guuuauuaua cgucuucgac gacauuauga augugguuaa uaaacgcuuc     3900

acacuauucg acaugucaaa auaugagaaa gauggugauu cacugagaga aaaauucaac     3960

ugucuagauu uuaacgacaa agucuccauu uuaucggauu uauuaaaagc auuccaugcc     4020

aauagcgaca gaacauccau aacaaaacuu aagaucacaa aucuaggaag acaccaggcc     4080

ggcaaaaaug gaauaacacu aacaacaaac gcacaaauaa uuuaucaguc cccaacagga     4140

cuauucgaga gacgcaucaa aauaaaagau cuauga                               4176


<210>  5561
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5561
augaaaaaaa ucuuagggcu agacuuaggu acaaauagua uaggaugggc auuagucaau       60

gaagcagaac augaacauga acaaucauca auaguuaagc uagggguaag aguaaaccca      120

cucuccgucg augaaaaaac gaacuucgaa aaaggaagac cacuaucaau aaaugccgau      180

cgcaccucua agagaagcgc acggagaaac uuacaaagau ucaaacucag acgcaaaaac      240

uuaauaaauc uccuguuaaa acaccacauc auaaguaaug aaaccauccu aacagaagua      300

gggaaaaaua cgacucacca aacucucaaa cuacgaucaa aagcuacgca agaaaaaaua      360

ucgcuagaag aauucgcgag aauacuauua ucaauaaaca aaaaaagagg auacaaaagu      420

ucuagaaagg agaaaaacga agaugaagga uucaccauug auggaauggc gaucgcaaaa      480

gagcuauacg auaaccaacu uacuccagga caauacguau auaacauacu ccaagacgga      540

aaaaauuaua uacccgacuu uuauagaagc gaccuacaag cugaauucga cgacguuugg      600

caauuccaga aaaaauuuua cuccuccauu cuaaccgaug aauucuaccu gaaauuaaaa      660

ggucagggua aagaaaauac caggaaacua uuccuugcaa uacaucaagu auacacggca      720

gagaauaaag gaaaacguga cgcagucaaa cuacaauauu acaaauggag aucagaagca      780

guaaaaaaac aacuauccau cgaagaagua gcuuacgucu uggucgaaau uaauaacaau      840

auaaauaaau cauccgguua ccuaggcgca auaagugauc gaaguaaggc acucuauuuu      900

aauaacgaga cgguaggaga auaccucuac ggucaauuag uuaaaaauag acauaacucg      960

uuaagaaauc aaguuuuuua ucgacaagac uacuuagacg aauuugaaaa gauaugggaa     1020

acccaagcaa aauuuuauga ugaauuaaca aacgaacuua aacaggaaau ucgagacgua     1080

guuauauucu aucaaagaaa acuaaaauca caaaaacacu uaauaaguga cuguuuauuc     1140

gagaaacacc auaaagcaau ccccaaaucc ucgccacuau uucaagaauu caaaauaugg     1200

caaaaucuca auuacuuaga auucgugcau acucaaucaa aagaaaaaau auucuucucc     1260

gaacuagacg augaaacucg acaauuuguc uucgccgaac uaaacauucg agggacacua     1320

aaaauuaaag aaauaaaaaa auuaaucgga uuagaaacac caguauauug gaagaguaac     1380

uucgacgaaa uagaaggaaa uagaacaaau caaguucugu acaauguaua ucaaaacaua     1440

gcagaugacu uaggauaugg auucgacugg ucaaagaaac augcguuuga aaucaaugcc     1500

gaguuacaag caauauuuaa agacguaggu auagacacag caauauuaaa cuucgaugcu     1560

ucaauagaag aaaaugauuu ugacaaacaa aaaucauauc aacucuggca uuuacuauac     1620

ucagcagaag auacagauaa agcuacagaa gaagacaaac uuauauacgg aaacauaaac     1680

acaaaacuga aaaagcaauu acaucagaaa uacggcuucg aggcuaaaua cggcaacuug     1740

cuggcaaaua ucucacuaca acaagacuau gguaaccuuu cagcaaaagc uauaaaaaaa     1800

auccuaccgu auuuagaauc uggaaacuca uuuuaugaag cgugcacaau cguuggauau     1860

aauccaucga auagccugac uaaagagcaa caacaaaaua gaaaacucaa agacaaacua     1920

gaauuauuac caaaaaacuc ucuacgaaac ccagucguag aaaaaauacu gaaccaaaaa     1980

guaaaccuaa ucaaccaacu aauugaugaa uacggaaaac cagacgaagu acguauagaa     2040

uuagcacgug aacuaaaaaa aagcgcaaaa cagagagccg accuaucgaa acacaucaac     2100

gacgccacac ucaagaacca gaauauacgc aagacacugc aaaaagacuu cggaauacaa     2160

aacccaucaa aaaacgaugu uauaagauau aaacuauacg augaacuuaa agauaacgga     2220

uauaaaaccc uauuuacaaa caaauacaua ccaaaagaac aacuauuuuc uaaagacguu     2280

gauauagaac acauaauacc aaaggcaaua cuauuugacg auucauuuuc aaauaaaaca     2340

uuagcauauc gaaacguuaa ucucgccaaa gcagacgcaa ccgcauuuga uuucauaucg     2400

agcaagcauc uagacgaccu agacaacuau aaggcacgcg uagaaacauu auuccaguca     2460

aagagaauaa gcaaaggaaa auacaaaaaa cucuuaauga cacgccaaaa uuuaccagaa     2520

gacuuuauag aacgggaucu ccgcaacuca caauacaucg caaagaaggc aacugcaaug     2580

cuaaaagaag uauuucgauc aguuguaccu acaucaggaa gaauaacaga caaacuaagg     2640

gaagacuggg accuagucaa uguaaugaaa gaguuaaauc uuccgaaaua ccgaacacuu     2700

ggauuaacag aauucauaga acgaaagaau gguaacaaaa uugaacaaau caucgacugg     2760

acgaaacgaa augaucauag acaccaugcu auggacgcgu uaacaguagc guuuaccaca     2820

cauaaucaua uacaauaccu gaacaaccua aauguaaccc acggccuaaa cggaguagac     2880

accgaucacg aaaaaagcag acuauacgca aucaaacaag ccaucacuaa gguauauauc     2940

guaggaaaaa ccaaaaaaag aaaguucaua ccacccaugg aaaauuuccg gagccacgcc     3000

aaaaaucauc uagaaggaau cuuaaucucu uucaaaaaca aaaauaaagu cguaaccaag     3060

aacaaaaaca gaaccaaaac caaaaccaaa ggaguguuca acgaaaaaau acaacugaca     3120

ccacgcggac aauugcacaa agaaaccauc uacggucaug caaaaaaggu uauaguaaca     3180

aaagaaaaag uauccucaaa auucacccac gaaaaaauau uacaagucac gaaaccugcc     3240

uaucgagaag cgcuacuaaa acgacuagca ucauucaaaa acgacccuaa aaaagcuuuc     3300

agcggaaaaa augcgcuaag uaagaacccg cuauacuucg auaucacaag uggaaaaaaa     3360

gucccagaca cggugaaauu gucauauuuc gaaaacucau acguaauaag aaaggaagua     3420

aacagcgaca auuuuaaaaa ugaaaaguca uuacaaaagg uauuagacgu aggaaucaga     3480

agaauccugg aaaaacgcuu agcugacuau aacggaaacg cuaaagaagc cuuuucaaac     3540

uuggagaaaa aucccaucug gaaagacaaa gaaaaaggag uauguauaaa aaagguaaga     3600

auagaagcuu uaaaaaacgc cgaaccacua caugucaaaa aagaucauuu aggcaaaauu     3660

auuuuagaug augagaacaa aucuacacca guggacuuca uauccacagg aaauaaccac     3720

cauguugcaa ucuaugaaga caaagacgga aaacuacacg auaagguagu uaguuucuac     3780

gaaguaguuu caagaguuaa ucuuggucua ccuaucaucg auaaauccuu caacucacac     3840

cucgauugga aauuucuuuu uucaaugaaa caaaacgaaa uguucguauu cccaagcgaa     3900

gacuuuaacc caaaagaaau cgauauagga aaucccaaaa auaaugaacu cauuuccaaa     3960

uacuuauuua gaguacaaaa guuuagcaaa guaacauaug gaaauagugc agucagagac     4020

uauguguuua gacaccauuu agaaaccgaa cuaauagaua aaaaggaauu aaaaggcaua     4080

acauacuacc aacuaaaauc aauagguaug cuagaaaaac uagucaaggu ccgcauaaau     4140

cauaucggca agauagucca cauaggugaa uacuga                               4176


<210>  5562
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5562
augaaaacga uauuaggucu ggacuuagga acgaauagca ucggaugggc ucuagucaaa       60

gaagcagaaa acgaaaauga aguauccgau auaauaaagc ucggaguacg uguuaacccc      120

uuaacagucg acgaauuaac aaauuucgaa ggagggaaag cauauagcaa caaugcagaa      180

agaacauuaa aacgcggcgc gagacgaaac cuacaacgau acaaauuaag aagagaaaac      240

cuaaucgaaa uuuuauuaaa gaauggucua auaaacaaag auacagcauu aacagaagua      300

ggaaaagaaa caacgcauca aacacucaaa cucaggagca aauccgcuaa cgaaaaaguu      360

ucacuagagg aucucgcaaa aaucuuacua agcauaaaca aaaagcgggg guauaaauca      420

ucgagaaagg uuaacgcgga agacgacgga guagcaauug acggaaugga agucgccaaa      480

cuacuguacg acgaaaacau gacacccgga gaauacguuu ugaggcuacu ggaaagugga      540

aagaaacauu uaccagacuu cuacagaagu gaucuacaag aagaguucaa ccuaguuugg      600

aagaaacaaa gugaguucua uccccuaaua uuaacaaaag aacucuucuu ugaacuacac      660

gggaaaaaua aaagucaaac augggcaaua ugcaaagaac cacuuggcau agucggagug      720

aaauuaaaag gcucggcaaa agaacaaaaa uuugcgcgau acaggcuacg aaaagaagcu      780

cuaacacagc aaauagaacu ggaacaccua gcaauaguau uacaggacau aaacaaugau      840

ucuaauaaaa guuccggcua cuuaggggcg auaagugauc gcagcaaaga auuauuuuuc      900

aauaaagaaa cuguaggaca auaccaauac aaacaacuac aaaaagaucc aaacacaagc      960

cuuaaaaacc aagucuucua ucgccaagac uaccuagaug aauuugaagu uauuuggcga     1020

aaucaagcaa aauucuaccc acaacuaaca aacgaacuaa aacaagaaau ccgagacgua     1080

guaauauuuu accaaagaaa acuaaaauca cagaaaaauc uaaucagcuu cugucaauuc     1140

gaaucaaccc aacaaaauua cguggaaaaa gaaacaggaa aaacaaaaau acgaacaaua     1200

ggcagaagag uaauuccuag guccucaccc uuauuucaag aauuuaaaau uuggcaaagu     1260

uugaacaauc ugaaauucgu aaauucagaa ucuaaccaaa uaauacaauu caucgaauua     1320

gaagaugaug uuaagaaagu aguauucgau gaacuaaauu uaagaggcga ucuaaaaccc     1380

aaccaagucc ucaaaaucuu auccaaauac augaaaauag gaaaacuaac acaauggaaa     1440

ugcaacuuag aagaaauaga gggcaauagg acaaauagug cacucuauaa ugucuuucaa     1500

augauagcag aagaagaagg cuacggauuu aauuggggua agaaaagcgc ucuugaaaua     1560

aaaucggaau uaaaggcaau auucaguagu auaggcauaa augauucaau acuugauuuc     1620

gauuuuucua agaaacuuga ucaacaacuc aguuaccaau uauggcauuu acucuacagc     1680

gcagaagagg gaaauucuaa aauuaccgaa gaagacaaau uuacauaugg aaacacaggg     1740

gucgcauuaa agaagaaacu acaucuuaaa uuuggauuca aaccagaaua caguacauua     1800

auaucgaacu uaagauuuac uucagacuau ggaaauuuaa guagcaaagc aauaucaaaa     1860

auuuuaccau uccuuagaga ugguucagau uauucaaaag ccugucaaua ugcaggcuac     1920

aaccacucga acucucuauc ugccgacgaa cuggauaaua gggaacuuaa accaaaauua     1980

gagcuucuca agaaaaauuc acuaagaaau ccgguaguag agaaaauccu aaaccaaaug     2040

guaaauguug uaaaccaagu aauagacaau uacggaaaac cagacgaagu ucggauagaa     2100

cuagcuaggg aacuaaagaa aagcgcuaaa gaaagaucac aaaugacgaa aaauauaucg     2160

gacgcuacac gacgaaacgc cgaaaucaag aaauauuugu caaaagaauu ugggauaccu     2220

aacccaacaa aaggagacgu aaucagauac agauuauggg aagaauuagc aucaaacggg     2280

uacaaagauu uauuccgcga uaaauauauc cccaaagaaa aacucuuuuc caacgaaaua     2340

gauauagaac acauaauacc uaaagcacua cuauuugaug acagcuucag uaauaaaaca     2400

cuagcauuca aaguugauaa ccuuaagaaa gcagaccgaa ccgcauuuga cuucauguca     2460

acugauuuuu caaaagaugu ugacagcuau aucgguagaa uagaaacauu acacaaagac     2520

ggaaaaauaa gcucaucaaa auacaagaaa uuaaugaucc ccaaaagcaa auuaguagau     2580

ggguuuauag aaagagaccu gagaaauaca caauacauag cuaaacaagc ccguaaaaug     2640

uuauuggaag uuuuuaaaac ugucguucca accacaggga aaaucacuga caaacuaaga     2700

gaagacuggg accuaaucaa uguuaugaaa gaacugaacu uccccaaaua uaaagcauua     2760

ggacucacua agaaaauaga aagauuugac guugggucag aaaagaucaa aucaauaaac     2820

guuauagagg gauggagcaa gagaaaugau cacagacauc acgccaugga ugcauuagca     2880

guagcuuuca caacacauaa ccacauccaa uaucuaaauu acuuaaacgc acgcaaagau     2940

auucaccacg aaaugcacgg aacuauaaaa ucaauagaaa aucaaaucau cgguaaagac     3000

gucaugggua aaagacguuu cauuccucca auaagcgauc uaagaaugaa agcaaaauua     3060

cagauagaaa guauuuugau aucguuuaaa aacaaaaaua aaguaguaac gugguccguc     3120

aacaaaacaa aauuaaaagg uaaagauaac uacaacaaac gaagacagcu aacaccacga     3180

gggcaacuac auaaagaaac aguguauggc aaaauaaagc ugaucgaucc caaaccauua     3240

aaacuaaaca auaaauucac uuuaguuaga gccagcauga uaguggacaa aguccacaga     3300

gagauaguac uucaacacuu auccaaauac gauaauaaca gcgagguugc cuuugauuca     3360

aagaccauca aaauagaccc aauccugaua gaaggaauug gacuaaaaga aguagugugc     3420

uacgaagacg ucuuuaaaau aagaaagaaa auaaguggug auaauuucaa aaacgaaaaa     3480

caccuagaaa aaguaauaga cgaaaaagua aaacaaauau uaaaagaccg ggucauaaag     3540

uuucagggca auuuuaagga agccuuuuca aaucuagaag aaaaucccau cugguuaaau     3600

aagaguaaag ggaucgcagu uaaaagugua gcuauaaagg ggguaaauaa ugcagaaagc     3660

cuacacgagg ggaaggauca uuucggcgac caaauacuug acaaaaacaa gaaacaaaua     3720

ccuguagacu uuguauccac uggaaacaac caucaugucg gaauauauga ggacaaagac     3780

ggcaaucugc aagaacgcgu ugugagcuuc uucgaggcag uaacaagagu caacgaaggc     3840

cuacccauaa uagacaaaac guauaacucu gacauaggau ggaaauuuaa auucacaaug     3900

aaacaaaaug aaauguucgu auucccuuca gaugauuuug acccuaauga auuugaccua     3960

acagacggua aaaaugcagc aaaaaucuca cccaaccuau accgaguaca gaaaauagca     4020

accaaaaacu auauguucag acaccaccua gaaacuguag ucacaaacga ccuugaguuc     4080

acauacaaau ccauccgauc uacuggaugg uuaagaaauc uaguuaaagu aagaauaaac     4140

cauuuaggcg aaauuguaua uguaggugaa uacuga                               4176


<210>  5563
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5563
augaaaaaaa uacuaggacu agaccuaggg acgaccucaa uagguugggc ccucguaaau       60

gaaaaagaaa acaacgaaga acuaaguucu auaauucgac uaggaguacg ggugaaccca      120

cucacuguug augaaaaaag caacuuugaa aaaggcaaag acauuacaac uaaugcagau      180

agaacgaaau guagaucagc uagacgaaac uuacaacgau uuaaacuacg aagggaagca      240

cuagccgacu gucuaaaacg acacggucua augacugacg aaacccccuu gugcgaaacg      300

aauaacucaa ucauauuuaa gaccuauagu aacagagcaa aagcagucug ugaaaaaaua      360

gcuuuagacg acuucgugag aguccucuuc augaucaaua aaaaaagagg auacaaaagu      420

agucgaaaau uaaacacaga ugacgaaggu acauuaguag augguaugga cguugcaaaa      480

uuauuauaca acgaacaaau uacaccaggu gaauaucuac uacgacucuu caaaaaagga      540

aaaaaaacca ggccagacuu uuauuuaagu gaccuaaaag ccgaacuaga aagaauaugg      600

gacuuccaaa gaacguucua uccagaaaua uuaugcaaug cacuaaaaga agaccucaaa      660

gggaaaucac gaacccaauc cucagcaauu uucuaugcaa aauacaaggu uagcacggcu      720

aaaaucgcag guaaagacaa aagacuacaa gagcuaacgu ggagaacaga cgcgcuacga      780

aagcaacuaa ccauagacga agucgcauau gucauaugca guauaaaugg cgaaguuaau      840

ggcaguucuc aaaggcuuuc aaugauuuca gaucggagua aacuacuaua cuuuaaccac      900

caaacaauag gacaauaucu aaugcaacga uuagaagaaa aucccaacgc aggcuuaaaa      960

aacauuauau uuuaccgaca agacuaugaa gaugaauuca acgauauuug gaacagccaa     1020

gcgaccuauc auaaagaacu aacgccagag uuaaaaaaag aaguaaaaga cauaauauuc     1080

uaccaaagag gccuuaaaag uugcaaaugg cugauaaauu auugugaacu agaacaugaa     1140

gaaauagaag uuaaaacacc ugaaggcaaa cugagaaaaa uaacaauagg uagcaaaaca     1200

uguccuaaaa gcucaccucu auuccaagaa uuuaaaauau ggcagguuuu aaauaauuua     1260

caaguuaguc cacucaauuc acgaagaaaa gcaaaaagaa aaaaagacga agaacccagc     1320

cuauucucag acgaggauau gaacacagca uguagguacu uaaacgaaga ggaaaaacug     1380

acgcuauuua aagaacuauc aauaaaaaag gagcuaaaga aaaaagacgc auuaaaacua     1440

uuguuugaaa acuauaaaga auuagaucua aauuauaaag uuauaagugg aaacguuacu     1500

auggcaaacu uauacagagc cuuucaagac auuauaucuu uaacaggcaa cggagaauuu     1560

gacuuccaag caaugccagc ggaagaagcu gaaaaaacag uagaggaaau auucgggggu     1620

cuaggauaua aaacagcuuu uuugcacuuu gaugccagcu uacaugacaa agaauuugaa     1680

cggcaaccgg cauaccgacu guggcaccuc cucuauucuu acgcuggaga ccaaucaaaa     1740

acgggaauag aagcauuagu aaaacgcaua caagaauuau guggauuuga accagaauac     1800

gcuagaauuc ucgcaaacau cuccuuuaua ccagacuacg gcaaccuuag uucuaaggcc     1860

augcguaaaa uccuacccua caugcaagac ggauacgagu auucugaagc augcagcaaa     1920

gcuggguaca ggcauucagc aaguagccug acaaaagaag aaauuaacaa aaaaaauuau     1980

aaaacucauu uagaugagau uaagagaaac agcuuaagaa acccuauugu cgaaaagaua     2040

cuuaaccaaa uggucaaugu aguaaacucc guaauagaua cuuauucaaa caucgaugaa     2100

aucagaauag aaauggcacg cgaguuaaaa aaaucaaagc cagaaaggga agacaugagc     2160

uucgcuauau cgaaagccac aaaagagaca gaaaggauca aaggaauacu agaacaagag     2220

uuuaagauac aacauguaac uagaaacgac auaauucguu accgauuaua ugaagaacua     2280

aaacccaaug gcuacaaaac acuauacuca aauacauaca ucccaagaga agaccucuuc     2340

ucuaaaaaau ucgauauaga acacauuaua ccaaaagcaa gauuauuuga cgacagcuac     2400

uccaacaaaa cauuagaacu acgagacaua aauauagaaa aagguaauca aacugccuac     2460

gauuuuguuc ucaguaaaua cggagaagaa ggacuguaca aauaugaaca acgcguagaa     2520

gaauuuaaaa agaauaacga acaagcuggc acaaaaugua gaaaauuacu gaugagagaa     2580

gccgauauac cuucagauuu ucucaauagg gauuuaaaug auucacagua uaucgcaaaa     2640

aaagcacaug aauuauuaag agaaauuguu ccuaccguaa cugcgacaac aggaagcaua     2700

acaaauagac uaagggaaga cuggaaacuc guuaauguca ugaaagaguu aaacugggaa     2760

aaauacgaua aauuaggccu uacugaagag uauaaagaca aagauggaaa caccguaaga     2820

cauauaaaag auuggacaaa aagaaacgac caucgacacc augcaaugga cgcccugaca     2880

auagccuuua ccaaaccuuc auucauucaa uacuuaaaua aucucaaugc cagaaguaac     2940

agagguggcu caauauaugg aauugaacaa aaagaacuug aaagagaaaa uaacaaauug     3000

guauucaucc caccgauaga uccaaaaaua uuccgaaagc aagcgaaaga acauuuaucg     3060

aacauauuaa uaucaauuaa agcuaaaaac aagguaguaa cuaaaaacgu uaacaagacu     3120

aaaaguaaau cuggaacaaa uagaaaggua caguuaacac cccgcggcca auuacaucua     3180

gagaccguuu acggcaaaau uagaacuuac aaaacugagg aaauuaaggu uggaacuaaa     3240

cuaacagaag agcuaauugc aaaagucgcc gauaaaaaga uuagagaagc ccugaugcaa     3300

cgccuaucag aauacggagg caacauaaaa aaagcauuuc aaaauauauc aaaacaucca     3360

auaugguuaa auggauccca uaccaaacau guaccagaaa aaguaaagau aguuuuuuuc     3420

gaggacguau ucacaauaag aaaagcaaua acaccagaca uaaaaauaga uaaaguuaua     3480

gacagcaaaa ucagacgcau auuacaagaa cgacucaacg aauuuggggg ggaagcuuca     3540

aaggccuuuu caaaucuaga cgaaaauccc auaugguuca accgagacaa gggaauagcu     3600

auuaagucug uaaaaauaag aggaauagcu aaaggcacag caauaagggu gaagaaagau     3660

auccacggaa aaaccauuau cggcaacaac ggauauccag uugcuaauga uuauguaaac     3720

accggcaaca aucaccaugu cgccaucuuu agggauaaag auggaaauuu gcaagagcac     3780

auuguaaguu uuuacgaagc aacagccaga gcgauucaaa accucccaau aguagaccga     3840

aauuacaaua gcgaaaaaga uuggaaauuc cuauucucaa ugaaacaaaa cgaauacuuu     3900

guauuuccaa auccaaacac aggauuuaac ccaucugaaa ucaguuuaaa agacgaaaaa     3960

aacuacgcau uaaucagucc caaucuuuuc agaguccaaa agcugaguca cuacuauuac     4020

guauuuagac aucaccuaga aacaaacgug gacacgaacc uacaauuaaa agauauaacc     4080

uggaagagaa uaacagcagu aaauaaccua acaggcgcag uuaaaguacg gauagaccac     4140

cuaggacgaa uuguugaugu gggagaauac aaguga                               4176


<210>  5564
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5564
augaagaacu accauaucgg auuagauaua ggaaccagcu caguaggauu ugcugcuaga       60

gaugaugaua auaaccuaau cagaguaaaa gguaaaaacg uaauaggagc acgacucuuu      120

ucugaaggac aaaccgcaca agaacguaga auggcacgaa cgucuaggag acgauauaac      180

agaagaaaau ggcgauuacg auuauuagau gagauauuuc acgacgaaau aauccacaaa      240

gacgcagagu ucuucaaucg cuuaaagcaa uccuuccucu caccaaaaga cgaaagaaaa      300

caauacuucg gcagccugcu uuucccagag acuggugacu cagaauauua ucauaauggu      360

aaccaaacaa uauaccaucu gcgacauaga cuuauaacuc aagaugaaaa agcagauaua      420

agagaaaucu acuuagcccu cagacacaua auaaaguaca gaggcaacuu ccucgaugac      480

acuccaguua gcagcuucga agcaucagau cuaaaacuag ccaguuucgu acccacaaua      540

aacgaucuau ucgaaaccau ugacguuaac uaugaauuuu caauggauaa caucaaugaa      600

auagaagaca uuuuauuaaa caauaaacuu cgaaacucag acaagucaaa gcaacucacc      660

gaccuccuaa cugaaccaau ugugaauaua gaugacaaaa gucuugaaaa acaauuuaau      720

aaagagagaa cagauauauc caaggaaauc gcuaaguccg ugguaggguc caagaugaac      780

guuaauaaac uacuuaguuu ugaaagcucc gaaggaucaa aaauagcuuu caguuuuucg      840

gaugcauccu cagaggacaa aaugauggaa cuaucagaua uccucgacga uagccgcaug      900

aauauauuaa acauccuuaa aguaaucuac ucaagagucc gccugaacca aauaauacca      960

aguggaaugg gacuaucgga aucaauggua gcagcguacg acucucauaa acaacaauua     1020

aaagaccuua agacacuacu uaacguucua ccuuucaaug aacgcuacgc aguaaaaauc     1080

gccuacgccg cuuacauagg aaauaucgac aaacaaaaau ucacuaaaag cgaaauuaag     1140

gcaaucgaca aauugaaaga gaaaagcgca auaaugaacg uacuagacuu uaaauccggc     1200

cauauaacua acgaagaacu aucaaacguc auaaaagccg uaauuguuga uaaauaccua     1260

aacgaugaag auuucgaaua ccacggaaua guagagaaaa uaaaccaaga uauuagcaau     1320

ggaagcuacc uacugaaaca acgcaacaac caaaauggaa acauucccca ccaacuccau     1380

caaauggaau uagaccaaau uauagaaaau caaucaaagu acuaccacuu ucuuggagaa     1440

cucaacccaa acucuaacag aaaucacaua gcaaaauaca aaauuucaga acuuguagcc     1500

uucaggguac cauacuacgu aggaccacuc auuacgccag aagaacaaga gaaaaccucc     1560

ggagccagcu ucgcauggau gaaaagaaag aaagacggug caauaacucc uuggaacuuc     1620

gaagaaaaag ucgacaaaga agaaaccgcg uccagauuca uaaaaagacu gaccguuaaa     1680

gacacauauu uacugaacga agaugucuua ccugauaaua gccuacuaua ugaaaaauuc     1740

aaagugcuaa acgaacuaaa cgucguaaaa gcaaacaaua agcgucucac aguagaagag     1800

aaacaaggag cauuugacaa uuuauucaag aaaaugaaaa caguaacagc aaaaagauua     1860

aagaacuacc uaguaucaaa ucacaaccac aucaacagug uagaaauaac aggacucuca     1920

gauggagaca aauuuaacaa uagcuuauca acauacauag acuucaagaa aaucuucgga     1980

gacaaaaucg acaacccagg ccuacuagau gauuucgagg acauaauaga guggucaaca     2040

gucuuugaag acagaaaaau cuuugaagaa aaagugagaa aagauauauc auggauaagu     2100

gaaagcgaaa uaaagucagu aguuaguaaa cgauauucug gcuggggucg auuaagcaag     2160

aaacuauuaa acugcauaac aaacaaugag ggacgaaaca ucaucgacga auuaugggag     2220

gaaucagaaa cauuuaugga agcaguauca aaaccagaag uaaaagaaca aauagacgga     2280

aucaauuccg acuucauaaa gaauacagau auagaaucag uccucgcuga cgcauacaca     2340

uccccucaaa acaagaaagc aaucagggaa acucacaaac uuguaaaaga cauacaaaaa     2400

gcgaugggug gccaagcccc ggcaaguaua ucuaucgaau uuacacguaa uccacaagac     2460

aaaggacaac uaacacgaag ccgacacucc gaaguaucaa gaaaauacaa agagaucagc     2520

aaagaaauau cgaaaacucu aaacgaugaa cuaaaaagua agaaaaauuc cauggaugug     2580

gacaaauacu aucucuacuu caugcaaaug ggcauagauc uguaugaugg uucaucaaua     2640

gacaucgaug auuugaauaa cuaucaaauu gaccacauaa uaccccaaag uuuuuacaaa     2700

gacgauucgu ucgacaauag aguacuaacu cauuauagca acaaccaaaa gaaaggaaau     2760

aacaccccac ucaacggccu aaacauaaac gauaaaacaa gggccauuug gaaucacuug     2820

cgagaccucg gcuuaauaac caaaaagaaa uacaauggcc ucacacuaaa agguauggac     2880

caauuaagca aauauacaaa acaugguuuu aucagaagac agcuaguaga aaccagucaa     2940

guaauaaagc uagucgcaaa cauacuaucc gaugaguucu caacagauga uacuaaaauu     3000

auagaaguca aagcuaaaau gaacucacaa auacgacaag uauuuaaucu cuauaaagua     3060

agagaauuga acgacuacca ucaugcggua gaugccuauc uaaccacguu uaucggagga     3120

uaccuauaca accgauaccc caacuuaaga aauauguucg uauacggcga cuucaaaaag     3180

uuuucagccg auguagacaa ucuaaagacu uuuaacuuuu uacacgaaau uacaaaucca     3240

ucagacaaau uugaagaucg aauauucgac gaaaaaucag gagaacuaau acuagacaga     3300

aaacacgcaa ucgaaacuau uaagaaaaua uaucaauaca aauauauguu aaucacuaga     3360

gaaguaucca cgaaaaaugg acaauuaagu gaucaaaccg uuuacucagc aaaaagaauu     3420

aauaauguaa aagcccccau ucccguaaag aaagacuuua acccagaacu auacggauuc     3480

cgaaccggaa acguagauca ccacauggug auugucaaau auccaaagaa agauagcuac     3540

gauuauaaag uggugggaau accacuuaga uucguacaaa aacucaaaga aacgaaagau     3600

aauaaagauu acgaaaaucu auuaaaucaa auugugcaaa ugaaacugaa cagaaagaaa     3660

caggacuuua uaguaguagu ggacaacgua auguacggac aacuuauuca ggacggggga     3720

cagaaauuca cccuagguuc aaguacuuac agauauaacg caaaacaacu uguaaucgac     3780

aauggauauc uaaaacuaau agccgacgaa agauauaaaa auagccuaac agaugaagaa     3840

aaaucaaacc aauacauaac auuauauaau gaaauccuaa aggugguaaa uggacgauuc     3900

gacguauuug auguaaacuc uuuucgaaag aaauuaaaua acggaguaga uaaauucaua     3960

aaacuuaaca cagucgauaa acgaauaauc auaaugaaaa uacuauccgg acuccacugu     4020

ggaccaucaa ucguccccuu aaaagaauua ggucuuacaa cgccucuagg ccaacuacaa     4080

ucaaccucag gaauaucucu cucggaaaac acuauaauua uuuaccaauc accaucaggc     4140

uuauucgaaa gaaaaguuag acucggcgau cuguga                               4176


<210>  5565
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5565
augaaaaacu accacauagg acucgacaua ggaaccucau caguuggcuu cgcagcacga       60

gaugaugaca acaaccuaau aaggguaaaa ggcaaaaacg uaaucggugc ccgauuauuu      120

ucagaaggua agaccgcaca ggaaagaagg auaucuagaa ccucccgcag acguuacaac      180

agacgaaaau ggcgacuacg auuacuagau gaaaucuucc acgaugccau cguacacaag      240

gacgcagagu ucuucaaccg auuaaaacaa ucaucacuau cuccaaaaga cgaacgcaaa      300

caauauuuug gaucucuacu cuucccggaa gucggagaug gggacuauuu ccgagauggu      360

aaccaaacca ucuaucacuu acgacaccgc cuaaugacac aagaugaaaa agcagacaua      420

agggaagugu accucgccuu aagacacauu auaaaauaca gagguaauuu ccuagaugau      480

accccaguau caucguucga agcauccgau cuuaaacuag cuaguuuugu accaacuuua      540

aaugaucuau uugaaacaau aaacguaaac uacgaauuau ccaucgacaa cguaaaucaa      600

auagaaaaua uucuauuaaa caauaaguua cgcaacucag auaagucuaa acaacucacc      660

gacuuaauag ccgaaccagu uacaaacaua gaugacaagu cuuuaaagaa acaauuugau      720

aaagaaagaa aagauauauc aaaagaaaua gcaaaagcag ucgucggauc aaaaaugaac      780

guuaacaaau uacucagcuu cgaaucaagu gaugggagua aaauaucauu uuccuucagc      840

gaugcaucau cugaagauaa aaugcucgaa cuuucugaca uuuuagauga uucaagaaug      900

aacauuuuga acguacuaaa agugauuuac ucacgaguga gacuuaauca aauuauaccc      960

ucaggaaugg gacuuaguga aucgaugaua gcagccuaug accuucauaa acaacaacua     1020

aaggaccuaa aaacccuuuu agacgucgua ccauucaacg aaaaguaugc agucaaaaua     1080

gcauacgcgg cuuauauagg aaacauagau aagcaaagau uuacaaaagc agaaguaaag     1140

acuauagaua aauuaagaga uaagaaaagc auuacaucag uauuagauuu uaaaagcgga     1200

cacauaacca acgaagaacu aaacaaugug auuaaagaag uaaucguuga uaaauacuua     1260

aacgacgaaa ccuucgaaua ccaugauauu guagagaaaa uaaaucaaga uauaucuaau     1320

ggcaacuacc uacuaaaaca aagaaauaac caaaauggaa acauaccuca ucaacuccac     1380

caaauggaac uugaucaaau uauagaaaaa caaucuaaau acuacccauu ccuuggaaaa     1440

cugaacccua acagcaaacg aagccauauu gccaaauaca aaauaucuga acucguagcc     1500

uuccguguac ccuauuaugu aggaccuuua auuacuccag aggaacaaga gaaaacuuca     1560

ggugccucau uugcuuggau gaaacgcaaa gaagacggag caauuacucc auggaauuuc     1620

gagaaaaugg ucgauaagga aagcacagcg uccagauuua uaaaaagacu uacaguaaaa     1680

gauacuuauc uccuaaauga agacguuuug ccugacaacu cacuacuaua ugaaaaguuc     1740

aaaguauuaa acgaacuaaa cguagucaaa gcgaauaaua agagauuaac uguagaagaa     1800

aaacaaggcg ccuuugacaa ccuauucaaa caaaugaaga caguaacggc uaaaagacua     1860

aagaacuauc uuguuucgaa ucauaaccau auuaauucag uaaaaaucac aggucuuucu     1920

gacggagaaa aauucaacaa caguuugagu guauauaucg auuuuaagaa gauauucggc     1980

gacaaaauag acgauuccaa auuauuugac gacuuugaaa acaucauaga auggucaaca     2040

guauucgaag auagagacau uuucgaagaa aaaguucgca aagauaucag uuggauaagc     2100

gaaagugaaa uaaacgcagu cgugucgaaa agauauucag gauggggacg acuaaguaag     2160

aaacuacuaa acggaaucac gaacgaugaa ggaagaaaua ucaucgacga auuaugggaa     2220

gaaagcgaaa ccuuuaugga agcaguuuca aaaccagaag ucaaagagaa aauagaugaa     2280

auaaauucaa auuucaucaa aaauaccgau auagaauccg uucuagcaga ugcuuacacc     2340

aguccccaaa auaagaaagc gauaagagaa acauauaaau uaguuaaaga cauccagaaa     2400

gcaaugggcg gacaagcucc cucuucaaua ucaaucgagu ucacuagaaa uccucaggac     2460

aaaggcgaac uaaccagauc caggcacagc gaaauugcaa agaaauacaa agaauuaucg     2520

aaagaaguua gcaagaaauu aaaugaugaa cuaaaaucaa agaaaaacuc uauggacgug     2580

gacaaauacu auuuauacuu uaugcaaaug ggcauagauu uauacgaugg uacuuccaua     2640

gauaucgaug aucuuaacaa uuaccaaauc gaccacauag uaccacaauc auuuuacaaa     2700

gacgacucau uugacaaucg cguacuaaca cacuacagca acaaccaaaa gaaaggaaac     2760

aauacuccuc uuaacggacu uaacauugau ucuaacacaa gggaaguaug gaaccaucuc     2820

aucggccuag gauuaauuac aaaaaggaaa uacaacaacc ucacacuaaa aggaauggac     2880

caacuuucaa aauauacaaa acauggcuuc aucagacguc agcuaguaga gacaagccaa     2940

gucauuaaau uaguagcuaa cauccuaucc gaugaguaua gcaaagauga uacuaaaauc     3000

auugaaguaa aagcuaaacu caacucacaa auacgacaag uauucaaucu cuacaaaauc     3060

agagaacuaa acgauuacca ccacgcacuc gacgcauacc uaacuacauu cauaggagga     3120

uacuuauaca auagauaccc aaaccuaaga aacauguuug uauauggaga uuucaagaaa     3180

uucucagcga aaauagauaa ccuagaaacc uuuaacuucc uacacgauau caccaauccc     3240

agugauaaau ucgaagacag aauaauagau gagaaaagug gugaaguaau ccuagauagg     3300

agacacgcga uagaaaccau caagaaaaua uaccaauaua acuauauguu aauaaccaga     3360

gaaguaucaa cuaaaaacgg gcaacuuucc gaucaaacug ucuauuccgc uaggagaauu     3420

aacaaaguca auucaccaau accaguaaag aaagauucca acccagaauu auacgguuuu     3480

cguaccggca acguagacca ccacaugguu auaguuaaau accucaacaa agacaaauau     3540

gauuacaaaa uuauaggaau accacuacga uuuguacaag uacucaaaaa uucuaacgac     3600

aacacagaau auaaaaacau ccuaaauaaa auuguccaaa gcaaacuaaa aggaaaaucg     3660

acugacuuuc gaguuauccu agacaacguu auguacggac aacuaauaca agacggcggg     3720

cagaaguuua cccuaggauc aucuaccuac aaauauaacg ccaaacaauu aguaauaaau     3780

auggaugaua uucgaauacu cguagacaac agauacuuca acucucuauc agaugaugac     3840

gcuaguggag aguucauaaa aauuuauaau gaaaucuuaa aaguaaucaa ugcccgcuuc     3900

gaccuuuucg acaucaauaa auuccgagaa aaacuaaaua auggcaaaga auacuuugaa     3960

aaucuaucca uagacgagaa aaaccaagua cuccuaaaaa ugcuaagugg ucuacauugc     4020

ggauccgcaa auguacccau aaaagaacua gggcuaacua caccacuagg aaaauuacaa     4080

auaccuagcg gaauaguacu aucagaagau gcaauaguca uauaucaauc acccaguggc     4140

uuauuugaac gaaaaaucag acuaaaagac auuuga                               4176


<210>  5566
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5566
augaacuuac guaauuaccc aggagaauac ucgauaggcu uagacauggg cacugggagc       60

guaggaugga gcguaguaga ugaaaaaggc aauuuauugc auuuuaaaaa gaaaccuaca      120

ugggggagca gacuuuuuga cuccguaaug ccagcuagug aagcacgcgu acaucgagga      180

caaagacggc gcuaugaaag aagaagaugg agauuggauu uauuacaagg ccuauucaaa      240

aaugaaauga acaaaguuga uccagaguuc uuuaugcguu uaaaccaauc aagaacuaua      300

gaaggagauc caaucuuuac aaaaucauuu accaaagcag acuacuacga cagguuuccg      360

acaauauauc auuuacgagc cuauuugaug gaaagcaacg agccagcuga cuuacgguua      420

auuuaccuag caaugcacaa caucaucaaa cauagaggua auuuccuucg cgaagaggag      480

aaaagucuca gugccaaaca cgcagacagu aagaaagcug uaacacaauu uguagacgcu      540

cuaaaagaau gguguuucag uaggggguau gaauuuggaa aagauccagc aaaaucuauu      600

gaaaaaugcu ugucugauag caaaucaucc agaucacaac uagcaaguga aauugccaaa      660

gcccuagcug ugucgaccga cgcggcugca gcagauaaga aauuagcaga uaauuuagcu      720

aaagccaugg uagguuugca augugaguuc aaauauguau ucggagaguu cgauugcgaa      780

aguacaaagu uagcacuuuc agacgacgaa aaacuagaac uuuuaagggc cgcauguccu      840

gaugacagcg ucgaacuacu cgacgcacua ugugcaguau auucagcaua cguauuacaa      900

ggucuacuaa guuaugcacc uggccaaaca auaucucaua auaugaucgc aaaauacgac      960

uuauauaaaa cugaucugaa aacuuuaaga gcauuaguga gggaauaugc accuggagca     1020

uacgaugagu ucuuuagagg agcaacuuac cacgacgcaa accccgacga uucuaguaaa     1080

gauuauaguu acgcagaugc ggaaggauac acagcuuaca auuuacauaa acugagcuau     1140

gaugacuuug caaaggcagu aaagaaacua uuagguggua cagaugcugu caccgaugaa     1200

agauauuuaa acaugaugga aagauuugaa aaacagcaau uucuucgaag acuaaaaacc     1260

agugacaacg gugccaucua cuaccaauua cacuuagaag aauuaacugc aauaaucaaa     1320

aaucaaggac gauuuuauuc uuuccuaaaa gaauaugaag gaaaauuuga gagcuuaguu     1380

agcuuucgaa uaccauauua uguaggacca cuuacaagug ucaaugcagc ccaagauucc     1440

cauaaucaua auagauucca augguccgaa agaaaaccag gaauggaaag agaaguaauu     1500

accccaugga auugggauac uauaauagau aaaaauaaaa gugcagagaa uuuuauaaug     1560

cguaugacug gagauuguac auauuuagca ggcgaaaaag uauuaccaaa acaaucacua     1620

uuauacgaag aauuuugcgu auuaaaugaa cugaauggag uaaggugguc agaugacgga     1680

gaugacuggc aacgauuuga ugcugcccaa agagaaggua uaguacgaga guuauuucac     1740

aaauacagga gggucacaua caaaauguua gaagauuggu uaguaagaga aggcgaugca     1800

aaccacccga uaguaaaagg aggucaaggg gaaacaggau uagaaagcaa aauggguagc     1860

uaccuauucu uugcgaaagg aauuuuugga cuaggagagu uaaguccggc ugaauauccu     1920

gcaauagaga aaaugauaaa auggaauaca cuuuuugaag auagaucaau auuaaaagaa     1980

aaauuagagg acgaguuugg agaaagaggu aauggcaugu uggaugcauc acagaucaaa     2040

gcaauaugua agaaaagauu cacugguugg ggaagacuca gugagcaauu ucuuacggga     2100

cugaaaguag aaagucccgc cggucgagcg guaaguauaa uggauguacu acgugaaggg     2160

aacccauguu cugaaagaag acagggugag acugugguua ugauggaagc acuucgcgac     2220

gaaggacugg gcuuccaaaa gaaaauagac gaacauaacc guaauuauua cggccaacau     2280

ggaaaagauu uaggagugaa ugagcuaccu ggaagcccug cgauucgcag aucacucaau     2340

caagcaaucc gaaucguuga agaaauagca ucgauagccg ggcaugcucc ugcuaauauc     2400

uucauugagg uaacucgggg agaagaugcu ggaaagaaag ggaaacgaac ugugaaaaga     2460

ugggauaaaa uuggagaagc acuaaagaaa uuaaaagagg aaggaguggc aguagaagua     2520

auggacgacu ucaaagaaca aaaagcuaua aacgcgaauu uggaugaaag acucagcuug     2580

uacuuaaugc aaaauggaaa augucuauau ucaggaaaag caauaaaccu agaaaaacua     2640

cuugcaggau caggagaaua cgaaguagac cauauaauuc cuagagcgua uaucaaagau     2700

gauagcauug aaaauaaagc ucuuguacug agagaagaaa aucagcacaa aacugaucaa     2760

uuauuaauag acaaaaguau cagauggaaa augagagaaa cauggaguca gcuacaugac     2820

gcuggacuaa uaggagauaa gaaauaucau aacuuacuac ggaguaacau aaacgagaac     2880

caacuuagag gauuuauugc gagacaauua guagagacuu cucaaauggu aaaacuugcg     2940

caagcacuac uugaagcacg uuuugccaau gaagaaacaa gaauaguacc gguuaaagcg     3000

ucaaugagcc acaaucuacg cgaagcggca gaccuugcga aaugccgaga agcuaacgac     3060

uuccaccacg cacaugacgc cuucuuagca uccaggauag guuuauucgu acaaaugaga     3120

cauccaggaa uauacgaaaa cccaauaggg uacagccacg uaauaaagaa auauguucaa     3180

gaacaaucug aagucuucaa gaaaacucac agaaugccug ggagugccgg auucguuaua     3240

aacaguuuua ugucaucagg cuucgacaaa gauaccggag aaguauucaa agacucaugg     3300

gaugcugaag cggagguaga ugguaucaga aaagcauuga acuauagaca augcuauaua     3360

ucacgaaugc cacaacaaga cacaggugca uucuggaaug caacggucua cucuccaaga     3420

gacccgaaaa uggggccaaa auuagcccuu ucuacaaaac agggacucga cccaaaauua     3480

uauggcgguu uuaguucaca acaauuugcu uauuucuuua uauaugaagc caaaaagaaa     3540

ggaaaaccaa cauuccgauu uucacaagug ccaguauggu uagcagcacg aauugaggca     3600

aacccgcuag cgcuugaaga auacgcaaga ggacuagcaa aaggagaggg ccuugaauuu     3660

guaucaauac agagaccaaa aaucuauaag aaacaacuca uagaaguaaa cggggauaaa     3720

cucauaauaa caggagcgaa agaggucaga aguggagcag agcuagcguu caaccaagau     3780

gaaauaucag caauaucaau guguucaaaa uuaagagcag cagaagcggg ucuagacggc     3840

gcauucucag aggaaguaag ugcaauaugg aaucgcauaa gcaacgcacc agugcaaagu     3900

ugugggcguu uacuaagaca acuaacacua cccaaauacc aguccaacuu ugacucaagu     3960

uccaacgaag aaaaagcggc aguaguauug gguuuaauag cccuauuaaa uggaaacaau     4020

cgaauagucg accuaucagg gguuggagga aguagccaug cuggagcuau gaugaugagu     4080

uauccuaaac uauuagacga cccuaaagug ccauuuuuca uaauugauca aagugucaca     4140

ggcauguuug aaagaaaaac ucgcguugga cuauga                               4176


<210>  5567
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5567
augaagaaaa uacuaggacu cgauuuaggc acuacaagca uaggcugggc ucuagucaac       60

gaaaaagaaa auaacgaaga acaauccagu aucauacgau ugggaguaag aguuaaccca      120

uugacagucg augaaaaauc gaauuucgaa aaaggcaaag auauuacaac aaacgcagac      180

agaacaaaau gcagaucagc aagacguaac cuccaaagau uuaaacugag aagggaagcc      240

uuagcugacu gccugaaaag acauggauua augacagaug aaacaccccu gugcgaaaca      300

aauaacucaa uaauauuuaa aacauauuca aaccgagcga aagccguuug ugagaaaauu      360

gcuuuagacg acuucguaag aguauuauuc augauaaaca agaaacgugg auacaaauca      420

ucaagaaaac uuaauaccga cgaugaagga acucuaguag acgguaugga cguagcgaaa      480

cuauuauaca augaacaaau cacaccagga gaauacuuac uaagauuauu uaagaaaggg      540

aagaaaacaa ggccugauuu uuaucuaagu gaucucaaag cagaacuuga aaggauaugg      600

gacuuucaac ggacauuuua cccagaaauc cuaugcgaug cauuaaaaga agaccucaaa      660

gguaaaaguc gcacgcaauc auccgccaua uuuuaugcua aauauaaagu aaguacagca      720

aaaauugcug gcaaagacaa aagacugcaa gaacucacuu ggaggacaga cgcccugaga      780

aaacaauuaa caauugacga aguagccuau guaauauguu cuauuaaugg agaaauaaac      840

ggcagcagcc aacgcuuaag uaugauuucu gaucgcucaa aacuauuaua cuucaaucac      900

caaacaauag gacaauaucu aaugcaaaga cuugaggaga acccaaaugc aggucuuaaa      960

aauauaauau uuuaccggca agauuaugaa gacgaauuua augauauaug gaauagccaa     1020

gcaacauacc auaaagagcu uacacccgaa uugaagaaag aacuaaaaga caucaucuuu     1080

uaccaacggg gauuaaaaag cugcaaaugg cuuaucaauu auugcgaacu ggaacacgaa     1140

gagauagaag uaaaaacacc cgaagguaaa uuaagaaaaa uaacuauagg gagcaaaaca     1200

ugcccaaaau cuagucccuu guuucaggag uucaaaauau ggcaaguacu uaacaaccua     1260

caaguuagcc cauugaacuc acguagaaaa gcaaaaagga agaaagacga ggaaccguca     1320

cuauucucug augaagauau gaacacagca ugucgcuacu uaaacgaaga agaaaaacuu     1380

acacuauuua aagaauuaag uauaaagaaa gaacucaaaa agaaagacgc acuaaaauua     1440

cuguucgaaa acuauaagga acuagaccuc aauuacaaag uaauaagugg caauguaacc     1500

auggccuccc uauacagagc cuuucaagac auuauaagcc uaacaggcaa uggagaauuu     1560

gauuuccaag ccaugcccgc agaagaagcc gagaaaacag uagaagaaau auuugguggg     1620

uuaggauaca agacagccuu uuuacacuuc gaugcaagcc uacacggaaa agaguucgaa     1680

agacaaccag cauauagauu auggcacuua cuauacucau augcggguga ccaaaguaaa     1740

acuggaauag aagcccuagu gaaaagaaua caagaauugu guggcuucga accagaauac     1800

gcacguauac uagcaaacau cucauucaua ccggacuacg gaaaccuuuc caguaaagcc     1860

augagaaaaa uucucccuua uaugcaagau ggauacgaau acucggaagc augcuccaaa     1920

gcuggauaua gacauagcgc uaguucacua acaaaagaag aaaucaacaa gaaaacauau     1980

aaaacucauc uagaugagau aaaaagaaac ucccucagaa auccaauagu agagaaaaua     2040

cuuaaccaga ugguaaaugu aguaaacagc gucauagaca cauacggguc aauagacgaa     2100

auucgaauug aaauggcuag agaacucaag aaaucgaaac ccgaacgcga agacauguca     2160

uucgcgauaa acaaagcuac aaaagaaacg gaacgaauca aaggaauacu agaacaagag     2220

uucaaaauuc aacacgucac ccguaacgac auaauacguu auagacuuua ugaagaacua     2280

aaaccuaaug gauauaaaac acuuuacucu aacacauaua uaccgagaga agauuuauuc     2340

agcaagaaau ucgacauuga acacauaaua ccaaaagcua ggcuauuuga cgacagcuac     2400

ucgaacaaaa cccuggaauu aagagauaua aacauugaaa aaggaaauca aacagccuac     2460

gauuucguac uaagcaaaua uggcgaagaa ggacuauaua aauacgagca aagaguagaa     2520

gaauuuaaga aaaauaacga gcaagcagga uccaaauguc gcaaauuacu aaugagagaa     2580

gcagacauuc ccuccgacuu ccucaacaga gaccuaaacg acagucaaua uauagcaaag     2640

aaagcacacg aacuccuaag ggaaauagua ccaacaguaa cagcaacuac agguagcauu     2700

acaaaccgau uaagggaaga cuggaaacuu guuaauguua ugaaagaacu caauugggaa     2760

aaauacgaua aauuaggccu aacagaagaa uacaaagaca aagauggaaa uacuguacga     2820

cacauaaaag acuggacuaa aagaaaugac caucgacauc acgcaaugga ugcauuaaca     2880

auagcauuca caaaaccgag cuuuauacaa uacuuaaaua auuuaaaugc cagaucaaac     2940

aaaggagggu cuaucuaugg aauagaacaa aaagaacuag aaagagaaaa caacaaauug     3000

guauuuauuc caccuauaga uccaaaaaua uucagaaaac aagcuaaaga acaccuauca     3060

aauauccuga ucucaauaaa agcuaaaaac aaaguuguaa cgaaaaacgu aaauaaaaca     3120

aaaaguaaau caggaacaaa ccgcaaagua cagcuaacac cucgagguca acuccaucua     3180

gagacaguau auggcaaaau aagaacauau aaaacagaag aaaucaaagu aggaacaaaa     3240

cugaccgagg aauuaauagc aaaaguugca gacaagaaaa ucagagaagc gcuaaugcag     3300

cgccucucug aauauggugg uaacauuaaa aaggcuuucc aaaauauauc aaaacauccg     3360

auauggcuaa acucaucaca cacaaaacau guaccagaaa aaguaaaaau agucuucuuc     3420

gaagauguau ucaccauucg aaaagcaaua accccugaca uuaaaauaga caaaguaauu     3480

aauuccaaaa uaagaaacau cuugcaggaa cgacucaaug aguuuggcgg agaagcagcg     3540

aaagcauuca gcaacuuaga ccaaaaucca auaugguuua acaaagauaa aggaauagcc     3600

auaaaauccg uuaaaaucag agguauagca aauggaacag caauaagagu uaagaaagau     3660

auacauggaa acacuauaau aggaaacaau ggauacccaa ucccaaauga uuacgucaau     3720

acaggaaaca aucaucaugu agccaucuuc cgagacaggg acggcaaccu gcaagaacac     3780

aucgucuccu uuuacgaagc aacagcccga gcaauccaaa accuuccuau agucgaccgc     3840

aauuacaacu cagagaaaga cugggaguuc cuauuuucca ugaaacaaaa ugaauauuuu     3900

guuuucccaa acccaaacac cggauucaau ccauuagaaa uagacuugcg aaacgaagaa     3960

aauuacgcau uaaucagccc caauuuauuc cgaguacaaa aauuguccca uuacuauuac     4020

guauuuagac aucaccuaga aaccaaugua gauacaaauu uacaauuaaa agacauaaca     4080

uggaaacgca uaaaagcaau uaauaaucuc accggcguag uuaaaguucg aaucgaccac     4140

uuaggcagaa ucguagacgu aggugaauau aaauga                               4176


<210>  5568
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5568
augaaaaaac gaauagacga uuauauacug ggacuagaua uagguacuaa cucaugcggg       60

ugggcuguca cagacaaaaa aaauaaccua cucaaacuua gagguaaaac agccaucggu      120

agccaccucu ucgaagaagg acacaccgca gccgaucgaa gaggguuccg gacaacccgu      180

agaagauuaa aacgacguaa auggcgacua agacuacuag aagaaauauu ugcagaaccu      240

auggccgaag ucgauccggg auuuuucgug agauugcacc aaucaugggu aaguccucua      300

gacaaaucca gaaaaaaaua ccaagccauu guauucccaa cugcuaaaga agaccaagaa      360

uacuaccaaa aauauccaac uauuuaucac cuacgagacg ccuugaugac ucaagacaga      420

caauucgauc uucgggaaau uuuucuugca auccaccaua uaguuaaaua caggggaaac      480

uuucuccagg auacggcagu caaagauuuu gaagcaucca agauagaagu aggucccauc      540

cuaacaaaua uaaacaaugc auucgcagac aaaauaguag aaggucagga ccuaauugaa      600

uuaaacgucg caaaugcugc cgaaauagag gcaguaauca ggggaaaaga ugcagacaaa      660

acacucuaua aaauggauaa agucaaaaaa auugcuaaac uacuaacaga uagcacagca      720

aaagagcaaa aaaacguagc uaaacaaaua gcaaacgcca ucaugggcua uaagacccaa      780

cucgaaacua uauuaggaaa agaaaucgau aaaaacgaca aagcgcaaug ggaauucaaa      840

cucucagacg cagaugauaa guuagacgcc uuacuaccug acuuagacga aagugaccaa      900

acaguagucg cagaaaucga aaaacuuuuu agcgcgauaa cacuuucuac cauaguagau      960

gaaaacaaau cucuaagcca aaguauggua gaaaaauaua acaaacacca ucaagacuac     1020

aaacugcuaa aggcguauau cgagaguuua ccagaccgug cacaagcaaa aaaauuacuc     1080

cuagcguaug aucuuuaugu aaacaauaga cauggacauc uccuagaagc caaaacagag     1140

uuuaaacaag auagcacuuu aacaaaggag gacuuuuaca aaacuguuaa ggacaacuua     1200

gacgacagug aauuagcaca uaaaauagca caggaaauug cagcggauaa uuuuaugcca     1260

aaacaaagaa cuaacagcaa uggaguaaua ccauuccaac uacaccaaau cgaacucgau     1320

aaaauaauag cuaaucaaag caaauacuac cccuucuuag cugcugaaaa cccaguagaa     1380

gaccaucgca aacaagcgcc cuacaaacua gaugaacuag uacgauucag agucccguau     1440

uauguaggcc caaugauaac ugcagacgaa caagaaaaaa caagcggaaa aucuuucgca     1500

uggaugguuc gaaaagagca aggacaaaua acccccugga auuucgaaca aaaaguagac     1560

cgacaagaau cggccaacaa auucauaaaa cgaaugacaa ucaaagauac cuaucuucuc     1620

ggugaggacg uacuccccgc uaacaguuua cuauaucagc gauuugaggu acuaaacgaa     1680

uugaauaaca uaagggucaa cggaucccgc auuucaguag acuuaaaaca gcagauuuuc     1740

aacgacuuau uugaagagaa aaaaaccguu accgaaaaag ccuuaauauc cuacuuaaag     1800

caaaaucucc aucuacccac aguugaaaua aaaggacucg cggacccaac aaaauucaac     1860

ucuagcuuag caucauauua ccaccuuaaa aacuuacacg uuuuugauaa agaauuagca     1920

gacccacaau accagaaaga auucgaaaaa auaauugagu acagcaguau uuucgaagac     1980

aaaaaaauau uucaagauaa auuacaugca gaauucaaau ggcuaacacc agcacaauuc     2040

aaagcuauca guacuuggcg acuacaaggc uggggacgac ucucaagaaa auuacuugua     2100

gaacuucacg auacuaaugg acaaaacauc aucgaacaac uaugggauuc acaaaaaaau     2160

uuuaugcaga uuguaaccga accugacuuc aaagaagcaa uugcuaaaga aaaucaaaau     2220

aucacacagg aaaauggcgu cgaagaaauc uuagccgacg cuuacaccuc cccugcuaau     2280

aaaaaagcaa uacgacaagu uguaaaagua guagcagacg ucgucaaagc agcuuccggu     2340

aaggcuccgg cacaauucgc cauugaauuc acccgugauc cggauaaaaa cccacaauug     2400

agccacauac ggggaaccaa auuauuaaag gcauaucaag aaaccgccgg ugaacuugua     2460

gaccagaacc ucacaaauag ccuaaaagaa gcuaugacaa guagaaaacu ccugaaagac     2520

aaauacuuuu uauauuucau gcaaacgggu cgggaugcau acaccggaaa aaaaauaaac     2580

auagacgaag uaucaacaaa cuaucaaaua gaccacauau uaccacagag uuuuaucaag     2640

gacgauagcu ucgauaaucg ugucuuaaca gcaacaccgc ucaacgcaga aaaaagcgau     2700

gacguucccu acaaacgauu ugcgaauaau uauguuucag aucuaaaaau gacaguagga     2760

gaaaugugga aacauuggca aaaagcugga auuauaaaua aacacaaacu cgguaauuua     2820

cuucuugacc cagauagacu aucaaaauuc caaaaauccg gauucaucaa ccgacaauua     2880

guugaaacau cacagaucgu aaaacuagua ucgguaauac uucagaauaa auauccagac     2940

gccgaaauca uaacaguuaa agcuggugau aacucugcac uuaggcagcg acuagauuua     3000

uacaaaucuc gagacguaaa ugauuaucac cacgcaauag acgccuauuu guccauagua     3060

ugugguaauu uuuuauauca aguauaucca aaauaccgac ccuauuucgu auauggccaa     3120

uucaaaaaau ucucccagga uccagaccuc caaaaagauc uaauaaaaca cuuuaaaggc     3180

uucaccuuca uguggccacu auuacaaaaa gauaacagug aacgaaaagc acuagaagaa     3240

auaaaagaaa auaacucaga ccgaauaguu uucuauaaac acccagacau auucgacaag     3300

uuaaggaaag cuuauaauua uaaauacaug cuagugucaa gagaaacgag cacugaaagu     3360

agcagcaugu uuaacaugac gauauacccu agaggagaaa gagauagaaa aaaaacaaga     3420

aaauuaauac ccaaagggaa cggucuagac ccaaaaauuu acggaggcua uucagguaac     3480

acagaugcau auauggucau cguuaaaaua gaaaaggcca aagagucaau auacaaaguc     3540

auaggaauuc cuaugagagc auuagcggga cuuaaacaag caaaaaauca aggaaacuau     3600

aaggaagaac uacacaaagu agucgaacca caaauaaugc uagauaaaaa ugggaaacca     3660

aaaaggggcg uggaaggauu cagaguagua aaagaccacg uaccauucaa acaaguaguc     3720

cuagacggag acaaaaaguu uaugcuguau ucugcgaaau augaaacuaa ugcuaaacaa     3780

uuaacguuau cccaagaaac aaugcgcauu guuacagaca aucuaaaaaa aucagaggac     3840

caagauaaac uacuaguuaa agccuaugac gaaaucuuac agaaagucga ccaguaccua     3900

ccgcuauucg auguuaauaa auuuagaaau agucuucauc ucggcagagc uaaauucuua     3960

gaucucgccg uaaaugauaa aaagauuaca cuaucaaaua uauuaaacgg acugcacgau     4020

aaucuuguag uaccaaaucu aaaaaauaua ggaauuaaaa cacccuuugg uuuaaugcaa     4080

aguccaagug gcaucacauu aucaucuaau gcaauauuaa ucuuucaaag uccuaccgga     4140

cuauucgaaa aacguguaag aaucgccgac cuauga                               4176


<210>  5569
<211>  4176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 variant

<400>  5569
auggagaaca aacaauauua cauuggacug gaugu